bluuebunny/crossref_metadata_2025 huggingface dataset:
Does Risk-Adapted Proton Beam Therapy Have a Role as a Complementary or Alternative Therapeutic Option for Hepatocellular Carcinoma?
Authors: Kim Tae Hyun , Park Joong-Won , Kim Bo Hyun , Kim Hyunjung , Moon Sung Ho , Kim Sang Soo , Woo Sang Myung , Koh Young-Hwan , Lee Woo Jin , Kim Dae Yong , Kim Chang-Min
Published: February 2019

To evaluate the role of risk-adapted proton beam therapy (PBT) in hepatocellular carcinoma (HCC) patients, a total of 243 HCC patients receiving risk-adapted PBT with three dose-fractionation regimens (regimen A [n = 40], B [n = 60], and C [n = 143]) according to the proximity of their gastrointestinal organs (<1 cm, 1–1.9 cm, and ≥2 cm, respectively) were reviewed: The prescribed doses to planning target volume 1 (PTV1) were 50 gray equivalents (GyE) (EQD2 [equivalent dose in 2 Gy fractions], 62.5 GyE10), 60 GyE (EQD2, 80 GyE10), and 66 GyE (EQD2, 91.3 GyE10) in 10 fractions, respectively, and those of PTV2 were 30 GyE (EQD2, 32.5 GyE10) in 10 fractions. In all patients, the five-year local recurrence-free survival (LRFS) and overall survival (OS) rates were 87.5% and 48.1%, respectively, with grade ≥3 toxicity of 0.4%. In regimens A, B, and C, the five-year LRFS and OS rates were 54.6%, 94.7%, and 92.4% (p < 0.001), and 16.7%, 39.2%, and 67.9% (p < 0.001), respectively. The five-year OS rates of the patients with the Modified Union for International Cancer Control (mUICC) stages I, II, III, and IVA and Barcelona Clinic Liver Cancer (BCLC) stages A, B, and C were 69.2%, 65.4%, 43.8%, and 26.6% (p < 0.001), respectively, and 65.1%, 40%, and 32.2% (p < 0.001), respectively. PBT could achieve promising long-term tumor control and have a potential role as a complementary or alternative therapeutic option across all stages of HCC.
Comparison of Diagnosis-Specific Survival Scores for Patients with Small-Cell Lung Cancer Irradiated for Brain Metastases
Authors: Rades Dirk , Hansen Heinke C. , Janssen Stefan , Schild Steven E.
Published: February 2019

Diagnosis-specific survival scores including a new score developed in 157 patients with brain metastases from small-cell lung cancer (SCLC) receiving whole-brain radiotherapy (WBRT) with 30 Gy in 10 fractions (WBRT-30-SCLC) were compared. Three prognostic groups were designed based on the 6-month survival probabilities of significant or almost significant factors, (age, performance score, number of brain metastases, extra-cerebral metastasis). Six-month survival rates were 6% (6–11 points), 44% (12–14 points) and 86% (16–19 points). The WBRT-30-SCLC was compared to three disease-specific scores for brain metastasis from SCLC, the original and updated diagnosis-specific graded prognostic assessment DS-GPA classifications and the Rades-SCLC. Positive predictive values (PPVs) used to correctly predict death ≤6 months were 94% (WBRT-30-SCLC), 88% (original DS-GPA), 88% (updated DS-GPA) and 100% (Rades-SCLC). PPVs to predict survival ≥6 months were 86%, 75%, 76% and 100%. For WBRT-30-SCLC and Rades-SCLC, differences between poor and intermediate prognoses groups and between intermediate and favorable prognoses groups were significant. For both DS-GPA classifications, only the difference between poor and intermediate prognoses groups was significant. Of these disease-specific tools, Rades-SCLC appeared to be the most accurate in identifying patients dying ≤6 months and patients surviving ≥6 months after irradiation, followed by the new WBRT-30-SCLC and the DS-GPA classifications.
Z-Schemed WO3/rGO/SnIn4S8 Sandwich Nanohybrids for Efficient Visible Light Photocatalytic Water Purification
Authors: Xu Pingfan , Huang Siyi , Liu Minghua , Lv Yuancai , Wang Zhonghui , Long Jinlin , Zhang Wei , Fan Haojun
Published: February 2019

Semiconductor photocatalysis has received much attention as a promising technique to solve energy crisis and environmental pollution. This work demonstrated the rational design of “sandwich” WO3/rGO/SnIn4S8 (WGS) Z-scheme photocatalysts for efficient purification of wastewater emitted from tannery and dyeing industries. Such materials were prepared by a combined protocol of the in situ precipitation method with hydrothermal synthesis, and structurally characterized by XRD, SEM, HRTEM, UV-vis DRS, and PL spectroscopy. Results showed that the Z-schemed nanohybrids significantly enhanced the photocatalytic activity compared to the single component photocatalysts. An optimized case of the WGS-2.5% photocatalysts exhibited the highest Cr(VI) reduction rate, which was ca. 1.8 and 12 times more than those of pure SnIn4S8 (SIS) and WO3, respectively. Moreover, the molecular mechanism of the enhanced photocatalysis was clearly revealed by the radical-trapping control experiments and electron paramagnetic resonance (ESR) spectroscopy. The amount of superoxide and hydroxyl radicals as the major reactive oxygen species performing the redox catalysis was enhanced significantly on the Z-scheme WGS photocatalysts, where the spatial separation of photoinduced electron–hole pairs was therefore accelerated for the reduction of Cr(VI) and degradation of Rhodamine B (RhB). This study provides a novel strategy for the synthesis of all-solid-state Z-scheme photocatalysts for environmental remediation.
Exosome in Cardiovascular Diseases: A Complex World Full of Hope
Authors: Bellin Gloria , Gardin Chiara , Ferroni Letizia , Chachques Juan Carlos , Rogante Massimo , Mitrečić Dinko , Ferrari Roberto , Zavan Barbara
Published: February 2019

Exosomes are a subgroup of extracellular vesicles containing a huge number of bioactive molecules. They represent an important means of cell communication, mostly between different cell populations, with the purpose of maintaining tissue homeostasis and coordinating the adaptive response to stress. This type of intercellular communication is important in the cardiovascular field, mainly due to the fact that the heart is a complex multicellular system. Given the growing interest in the role of exosomes in cardiovascular diseases and the numerous studies published in the last few decades, we focused on the most relevant results about exosomes in the cardiovascular filed starting from their characterization, passing through the study of their function, and ending with perspectives for their use in cardiovascular therapies.
CVm6A: A Visualization and Exploration Database for m6As in Cell Lines
Authors: Han Yujing , Feng Jing , Xia Linjian , Dong Xin , Zhang Xinyang , Zhang Shihan , Miao Yuqi , Xu Qidi , Xiao Shan , Zuo Zhixiang , Xia Laixin , He Chunjiang
Published: February 2019

N6-methyladenosine (m6A) has been identified in various biological processes and plays important regulatory functions in diverse cells. However, there is still no visualization database for exploring global m6A patterns across cell lines. Here we collected all available MeRIP-Seq and m6A-CLIP-Seq datasets from public databases and identified 340,950 and 179,201 m6A peaks dependent on 23 human and eight mouse cell lines respectively. Those m6A peaks were further classified into mRNA and lncRNA groups. To better understand the potential function of m6A, we then mapped m6A peaks in different subcellular components and gene regions. Among those human m6A modification, 190,050 and 150,900 peaks were identified in cancer and non-cancer cells, respectively. Finally, all results were integrated and imported into a visualized cell-dependent m6A database CVm6A. We believe the specificity of CVm6A could significantly contribute to the research for the function and regulation of cell-dependent m6A modification in disease and development.
Thermoelectric Properties of Thin Films of Germanium-Gold Alloy Obtained by Magnetron Sputtering
Authors: Nowak Damian , Turkiewicz Marta , Solnica Natalia
Published: February 2019

In this paper, the electric and thermoelectric properties of thin films of germanium–gold alloy (Ge–Au) are discussed in terms of choosing the optimal deposition process and post-processing conditions to obtain Ge–Au layers with the best thermoelectric parameters. Thin films were fabricated by magnetron sputtering using the Ge–Au alloy target onto glass substrates at two various conditions; during one of the sputtering processes, the external substrate bias voltage (Ub = −150 V) was used. After deposition thin films were annealed in the atmosphere of N2 at various temperatures (473, 523 and 573 K) to investigate the influence of annealing temperature on the electric and thermoelectric properties of films. Afterwards, the thermocouples were created by deposition of the NiCrSi/Ag contact pads onto Ge–Au films. In this work, particular attention has been paid to thermoelectric properties of fabricated thin films—the thermoelectric voltage, Seebeck coefficient, power factor PF and dimensionless figure of merit ZT were determined.
Using Conjoint Analyses to Improve Cable Yarder Design Characteristics: An Austrian Yarder Case Study to Advance Cost-Effective Extraction
Authors: Kühmaier Martin , Harrill Hunter , Ghaffariyan Mohammad Reza , Hofer Manfred , Stampfer Karl , Brown Mark , Visser Rien
Published: February 2019

Steep country harvesting has been identified as the main bottleneck to achieving greater profitability in the forestry sector of New Zealand and Australia. An improvement of efficiency, work safety and environmental sustainability should be realized by developing an advanced steep terrain timber harvesting system based on innovative Austrian technology. To identify the best suitable configuration of a cable yarder for steep terrain harvesting, user preferences based on an online survey (conjoint analysis) have been evaluated to answer the following questions: (1) What attributes of a new yarder design are most important to consumers? (2) Which criteria do stakeholders consider when selecting a cable yarder? (3) What are the weights representing the relative importance of criteria? Using eight specific design scenarios a fourth question, being which cable yarder concept is the best, was also answered. This case study shows that conjoint analyses is an effective tool to assess, rate and subsequently integrate design characteristics. Based on the results of the analysis, a cable yarder prototype will be manufactured in Austria and transferred to New Zealand for testing and demonstration.
Decreased Temperature with Increasing Elevation Decreases the End-Season Leaf-to-Wood Reallocation of Resources in Deciduous Betula ermanii Cham. Trees
Authors: Cong Yu , Li Mai-He , Liu Kai , Dang Yong-Cai , Han Hu-Dong , He Hong S.
Published: February 2019

Global air temperature has increased and continues to increase, especially in high latitude and high altitude areas, which may affect plant resource physiology and thus plant growth and productivity. The resource remobilization efficiency of plants in response to global warming is, however, still poorly understood. We thus assessed end-season resource remobilization from leaves to woody tissues in deciduous Betula ermanii Cham. trees grown along an elevational gradient ranging from 1700 m to 2187 m a.s.l. on Changbai Mountain, northeastern China. We hypothesized that end-season resource remobilization efficiency from leaves to storage tissues increases with increasing elevation or decreasing temperature. To test this hypothesis, concentrations of non-structural carbohydrates (NSCs), nitrogen (N), phosphorus (P), and potassium (K) during peak shoot growth (July) were compared with those at the end of growing season (September on Changbai Mt.) for each tissue type. To avoid leaf phenological effects on parameters, fallen leaves were collected at the end-season. Except for July-shoot NSC and July-leaf K, tissue concentrations of NSC, N, P, and K did not decrease with increasing elevation for both July and September. We found that the end-season leaf-to-wood reallocation efficiency decreased with increasing elevation. This lower reallocation efficiency may result in resource limitation in high-elevation trees. Future warming may promote leaf-to-wood resource reallocation, leading to upward shift of forests to higher elevations. The NSC, N, P, and K accumulated in stems and roots but not in shoots, especially in trees grown close to or at their upper limit, indicating that stems and roots of deciduous trees are the most important storage tissues over winter. Our results contribute to better understand the resource-related ecophysiological mechanisms for treeline formation, and vice versa, to better predict forest dynamics at high elevations in response to global warming. Our study provides resource-related ecophysiological knowledge for developing management strategies for high elevation forests in a rapidly warming world.
Tooth-Marked Tongue Recognition Using Gradient-Weighted Class Activation Maps
Authors: Sun Yue , Dai Songmin , Li Jide , Zhang Yin , Li Xiaoqiang
Published: February 2019

The tooth-marked tongue is an important indicator in traditional Chinese medicinal diagnosis. However, the clinical competence of tongue diagnosis is determined by the experience and knowledge of the practitioners. Due to the characteristics of different tongues, having many variations such as different colors and shapes, tooth-marked tongue recognition is challenging. Most existing methods focus on partial concave features and use specific threshold values to classify the tooth-marked tongue. They lose the overall tongue information and lack the ability to be generalized and interpretable. In this paper, we try to solve these problems by proposing a visual explanation method which takes the entire tongue image as an input and uses a convolutional neural network to extract features (instead of setting a fixed threshold artificially) then classifies the tongue and produces a coarse localization map highlighting tooth-marked regions using Gradient-weighted Class Activation Mapping. Experimental results demonstrate the effectiveness of the proposed method.
Joint Optimal Power Allocation and Relay Selection Scheme in Energy Harvesting Two-Way Relaying Network
Authors: Song Xin , Xu Siyang , Xie Zhigang , Han Xiuwei
Published: February 2019

In this paper, we propose a joint power allocation, time switching (TS) factor and relay selection scheme for an energy harvesting two-way relaying communication network (TWRN), where two transceivers exchange information with the help of a wireless-powered relay. By exploiting the TS architecture at the relay node, the relay node needs to use additional time slots for energy transmission, reducing the transmission rate. Thus, we propose a joint resource allocation algorithm to maximize the max-min bidirectional instantaneous information rate. To solve the original non-convex optimization problem, the objective function is decomposed into three sub-problems and solved sequentially. The closed-form solution of the transmit power of two sources and the optimal TS factor can be obtained by the information rate balancing technology and the proposed time allocation scheme, respectively. At last, the optimal relay node can be obtained. Simulation results show that the performance of the proposed algorithm is better than the traditional schemes and power-splitting (PS) scheme.
Commentary on Sequestering Atmospheric CO2 Inorganically: A Solution for Malaysia’s CO2 Emission
Authors: Gallagher John Barry , Nilamani Nithiyaa , Zanuri Norlaila Binti Mohd
Published: February 2019

The commentary questions the basis behind an article on accounting and calculating inorganic carbon sequestration services for Malaysia. We point out the omission of coastal vegetated ecosystems. We also bring the author’s attention to the problems of using a seemingly resultant chemistry within open systems, in which reactive species come from external sources. In addition, we point out that ecosystem services in the mitigation of climate change must be referenced against a manufacturing process, such as cement’s normal lifetime of carbon dioxide sequestration. Without such a reference state, sequestration services may be severely overestimated and when used within a cap and trade system, it will lead to an increased rate of carbon dioxide emissions.
The Rise of A Habitable Planet: Four Required Conditions for the Origin of Life in the Universe
Authors: Kompanichenko Vladimir
Published: February 2019

The advanced version of the author’s inversion concept of the origin of terrestrial life and its application for life in the Universe has been substantiated. A key step in the transition to life consists in the thermodynamic inversion of non-living prebiotic microsystems when the contributions of free energy (F) and information (I) become prevalent over the contribution of entropy (S). It is based the thermodynamic corridor that is mandatory for all chemical scenarios for the origin of life: F + I < S (prebiotic microsystem) → F + I ≈ S (intermediate stage, inversion moment) → F + I > S (primary living unit). A prebiotic organic microsystem can reach the intermediate state between non-life and life only under high-frequency and multilevel oscillations of physic-chemical parameters in hydrothermal environments. The oscillations are considered the fourth required condition for the origin of life, in addition to the three well-known ones: the availability of organic matter, an aqueous medium, and a source of energy. The emergence of initial life sparks in nonequilibrium prebiotic microsystems (being at the intermediate state) proceeds through the continuous response (counteraction) of prebiotic microsystems to incessant physic-chemical oscillations (stress). The next step of laboratory simulations on the origin of life directed to the exploration of the microsystems’ response to high-frequency oscillations (>10−10 s–<30 min) is proposed. Finally, some fragments of the general scenario of the origin of life in the Universe based on the whole four required conditions have been outlined.
Assessment of Terrigenous Nutrient Loading to Coastal Ecosystems along a Human Land-Use Gradient, Tutuila, American Samoa
Authors: Shuler Christopher K. , Amato Daniel W. , Gibson Veronica , Baker Lydia , Olguin Ashley N. , Dulai Henrietta , Smith Celia M. , Alegado Rosanna A.
Published: February 2019

Anthropogenic nutrient loading is well recognized as a stressor to coastal ecosystem health. However, resource managers are often focused on addressing point source or surface water discharge, whereas the impact of submarine groundwater discharge (SGD) as a nutrient vector is often unappreciated. This study examines connections between land use and nutrient loading through comparison of four watersheds and embayments spanning a gradient of human use impact on Tutuila, a high tropical oceanic island in American Samoa. In each study location, coastal radon-222 measurements, dissolved nutrient concentrations, and nitrogen isotope values (δ15N) in water and in situ macroalgal tissue were used to explore SGD and baseflow derived nutrient impacts, and to determine probable nutrient sources. In addition to sampling in situ macroalgae, pre-treated macroalgal specimens were deployed throughout each embayment to uptake ambient nutrients and provide a standardized assessment of differences between locations. Results show SGD-derived nutrient flux was more significant than baseflow nutrient flux in all watersheds, and δ15N values in water and algae suggested wastewater or manure are likely sources of elevated nutrient levels. While nutrient loading correlated well with expected anthropogenic impact, other factors such as differences in hydrogeology, distribution of development, and wastewater infrastructure also likely play a role in the visibility of impacts in each watershed.
Re-Evaluating Expertise: Principles for Food and Nutrition Security Research, Advocacy and Solutions in High-Income Countries
Authors: Gallegos Danielle , Chilton Mariana M.
Published: February 2019

Drawing on examples from Australia and the United States, we outline the benefits of sharing expertise to identify new approaches to food and nutrition security. While there are many challenges to sharing expertise such as discrimination, academic expectations, siloed thinking, and cultural differences, we identify principles and values that can help food insecurity researchers to improve solutions. These principles are critical consciousness, undoing white privilege, adopting a rights framework, and engaging in co-creation processes. These changes demand a commitment to the following values: acceptance of multiple knowledges, caring relationships, humility, empathy, reciprocity, trust, transparency, accountability, and courage.
Perception of Environmental Risks and Behavioral Changes during Pregnancy: A Cross-Sectional Study of French Postpartum Women
Authors: Teysseire Raphaëlle , Lecourt Marion , Canet Jim , Manangama Guyguy , Sentilhes Loïc , Delva Fleur
Published: February 2019

Limiting exposure to environmental hazards during preconception and pregnancy is essential for preventing adverse pregnancy outcomes or developmental defects in offspring. However, the perception of environmental risk and the behavioral changes of women planning or having a pregnancy have rarely been investigated, except for a few risk factors. We thus performed a cross-sectional study of French postpartum women hospitalized in the Bordeaux University Hospital in 2017 by proposing a self-administrated survey. The main objective was to assess their level of awareness concerning a large panel of environmental hazards and modifications in their behavior during pregnancy in occupational and household environments. Among the 121 respondents, most identified the environment as a major factor for a healthy pregnancy but recognized a lack of knowledge regarding environmental risk factors. The internet, television, and magazines were their main sources of information. Most women modified some of their practices at work or home. These measures were rarely implemented in consultation with a health practitioner, which raises concerns about the relevance of the adjustments made. Our findings highlight the need to improve the quality of information available to women and to help them implement preventive measures in consultation with physicians.
Trends in Alcohol Intake and the Association between Socio-Demographic Factors and Volume of Alcohol Intake amongst Adult Male Drinkers in China
Authors: Liu Ruiyi , Chen Li , Zhang Fan , Zhu Rui , Lin Xinjie , Meng Xuchen , Li Huabing , Lei Xun , Zhao Yong
Published: February 2019

Background: The volume of alcohol intake and type of alcohol affect Chinese men’s health. This study investigated changes of alcohol type between 2004 and 2011, explored the trend of change in alcohol type with age and determined the social demographic factors influencing the alcohol intake of Chinese men. Methods: Research data originated from the public database, China Health and Nutrition Survey (CHNS). Three chi-square tests were used to determine the prevalence of different alcohol types (beer, wine and liqueur) and the trend with age among male drinkers from 2004 to 2011. An ordered logistic regression model was established with alcohol intake as the dependent variable and social demography as the independent variable to analyze the influence of these factors on male alcohol intake. Results: This study confirmed that from 2004 to 2011, 70.1% of Chinese men consumed alcohol less than 168 g/w. The popularity of beer was on the rise, while the liqueur alcohol consumption decreased from 2004 to 2011 and the consumption of wine began to rise rapidly after 2006 (p < 0.05 for all). The prevalence of liqueur drinking increased with age and the prevalence of beer drinking decreased with age among Chinese male drinkers (p < 0.05 for all). From 2004 to 2011, a positive correlation appeared between age and male alcohol intake (p < 0.05 for all). In 2004 (OR = 1.22, 95% CI: 1.03–1.44), 2006 (OR = 1.21, 95% CI: 1.02–1.42) and 2011 (OR = 1.51, 95% CI: 1.31–1.75), Chinese men living in rural areas had a high volume of alcohol intake. From 2004 to 2011, the participants had married consumed more alcohol (p < 0.05 for all). In 2004 (OR = 0.61, 95% CI: 0.43–0.88) and 2011 (OR = 0.80, 95% CI: 0.68–0.94), higher education levels were negatively correlated with male alcohol intake. In 2006 (OR = 1.29, 95% CI: 1.07–1.56), 2009 (OR = 1.76, 95% CI: 1.45–2.14) and 2011 (OR = 1.35, 95% CI: 1.13–1.61), male drinkers who were working consumed more alcohol. From 2004 to 2011, a significant positive correlation appeared between tobacco consumption and alcohol intake (p < 0.05 for all). Conclusion: Consumption of three types of alcohol (beer, wine and liqueur) varies with the year. Beer consumption decreases with age, whereas liqueur consumption increases with age. Social demographic factors, such as residence, age, highest education level, working status and tobacco consumption, are related to alcohol intake. Our study affirms the effect of age on the choice of different types of alcohol.
Perceived Risk of Genetically Modified Foods Among Residents in Xi’an, China: A Structural Equation Modeling Approach
Authors: Zhang Wenjing , Xue Jianhong , Folmer Henk , Hussain Khadim
Published: February 2019

This paper applies a structural equation modeling approach to study the formation of consumers’ perceived health risk of genetically modified (GM) foods based on a cross-sectional dataset of 508 consumers in Xi’an, China. The results indicate a high percentage of respondents who believe that GM foods might threaten human health. The estimated structural equation model shows that males, older people, respondents with higher income, those with better educational attainment, and those with family members who need special care have higher perceived risks of GM foods. Effective risk communication is necessary to provide consumers with scientific information about GM foods in order to facilitate their understanding of the actual risks of GM foods.
Does Eating-Away-from-Home Increase the Risk of a Metabolic Syndrome Diagnosis?
Authors: Wang Hui , Yu Yingjie , Tian Xu
Published: February 2019

Rising frequency of eating-away-from-home (EAFH) is suspected to be correlated with several non-communicable diseases. This study adopted the Chinese Health and Nutrition Survey (CHNS) 2009 data to investigate the association between being diagnosed with the metabolic syndrome (MetS) and EAFH at different ages. Results showed that the association between EAFH and MetS varied at different ages and differed for males and females. EAFH was positively associated with a higher risk of getting MetS for males, especially for those aged between 45 and 60; while it was negatively associated with the risk of getting MetS for young females (<45) (all p < 0.05). In particular, EAFH was associated with a lower risk of getting high serum triglycerides (TGs), abdominal adiposity, elevated blood pressure, and impaired fasting blood glucose for young females, while higher risk of high serum TGs, abdominal adiposity, elevated blood pressure, and impaired fasting blood glucose for middle-aged males (all p < 0.05). In addition, a higher frequency of EAFH was associated with a higher risk of abdominal adiposity and elevated blood pressure for older women, and a lower risk of elevated blood pressure, and impaired fasting blood glucose for younger men (all p < 0.05). Our study implies that heterogeneous target strategies for preventing MetS in different subpopulation should be considered.
A Vehicle Routing Optimization Problem for Cold Chain Logistics Considering Customer Satisfaction and Carbon Emissions
Authors: Qin Gaoyuan , Tao Fengming , Li Lixia
Published: February 2019

Under fierce market competition and the demand for low-carbon economy, cold chain logistics companies have to pay attention to customer satisfaction and carbon emissions for better development. In order to simultaneously consider cost, customer satisfaction, and carbon emissions in the cold chain logistics path optimization problem, based on the idea of cost–benefit, this paper proposes a comprehensive cold chain vehicle routing problem optimization model with the objective function of minimizing the cost of unit satisfied customer. For customer satisfaction, this paper uses the punctuality of delivery as the evaluation standard. For carbon emissions, this paper introduces the carbon trading mechanism to calculate carbon emissions costs. An actual case data is used with a cycle evolutionary genetic algorithm to carry out computational experiments in the model. First, the effectiveness of the algorithm and model were verified by a numerical comparison experiment. The optimization results of the model show that increasing the total cost by a small amount can greatly improve average customer satisfaction, thereby obtaining a highly cost-effective solution. Second, the impact of carbon price on total costs, carbon emissions, and average customer satisfaction have also been numerically analyzed. The experimental results show that as carbon price increases, there are two opposite trends in total costs, depending on whether carbon quota is sufficient. Increasing carbon price within a certain range can effectively reduce carbon emissions, but at the same time it will reduce average customer satisfaction to a certain extent; there is a trade-off between carbon emissions and customer satisfaction. This model enriches the optimization research of cold chain logistics distribution, and the study results complement the impact research of carbon price on carbon emissions and customer satisfaction. Finally, some practical managerial implications for enterprises and government are offered.
Associations between Urban Green Spaces and Health are Dependent on the Analytical Scale and How Urban Green Spaces are Measured
Authors: Zhang Liqing , Tan Puay Yok
Published: February 2019

Although the benefits from exposure to urban green spaces (UGS) are increasingly reported, there are important knowledge gaps in the nature of UGS-health relationships. One such unknown area is the dependence of UGS-health associations on the types of UGS studied, the way they are quantified, and the spatial scale used in the analysis. These knowledge gaps have important ramifications on our ability to develop generalizations to promote implementation and facilitate comparative studies across different socio-cultural and socio-economic contexts. We conducted a study in Singapore to examine the dependence of UGS-health associations on the metrics for quantifying UGS (vegetation cover, canopy cover and park area) in different types of buffer area (circular, nested and network) at different spatial scales. A population-based household survey (n = 1000) was used to collect information on self-reported health and perception and usage pattern of UGS. The results showed that although all three UGS metrics were positively related to mental health at certain scales, overall, canopy cover showed the strongest associations with mental health at most scales. There also appears to be minimum and maximum threshold levels of spatial scale at which UGS and health have significant associations, with the strongest associations consistently shown between 400 m to 1600 m in different buffer types. We discuss the significance of these results for UGS-health studies and applications in UGS planning for improved health of urban dwellers.
Estimating Ground-Level Concentrations of Multiple Air Pollutants and Their Health Impacts in the Huaihe River Basin in China
Authors: Zhang Deying , Bai Kaixu , Zhou Yunyun , Shi Runhe , Ren Hongyan
Published: February 2019

Air pollutants existing in the environment may have negative impacts on human health depending on their toxicity and concentrations. Remote sensing data enable researchers to map concentrations of various air pollutants over vast areas. By combining ground-level concentrations with population data, the spatial distribution of health impacts attributed to air pollutants can be acquired. This study took five highly populated and severely polluted provinces along the Huaihe River, China, as the research area. The ground-level concentrations of four major air pollutants including nitrogen dioxide (NO2), sulfate dioxide (SO2), particulate matters with diameter equal or less than 10 (PM10) or 2.5 micron (PM2.5) were estimated based on relevant remote sensing data using the geographically weighted regression (GWR) model. The health impacts of these pollutants were then assessed with the aid of co-located gridded population data. The results show that the annual average concentrations of ground-level NO2, SO2, PM10, and PM2.5 in 2016 were 31 µg/m3, 26 µg/m3, 100 µg/m3, and 59 µg/m3, respectively. In terms of the health impacts attributable to NO2, SO2, PM10, and PM2.5, there were 546, 1788, 10,595, and 8364 respiratory deaths, and 1221, 9666, 46,954, and 39,524 cardiovascular deaths, respectively. Northern Henan, west-central Shandong, southern Jiangsu, and Wuhan City in Hubei are prone to large health risks. Meanwhile, air pollutants have an overall greater impact on cardiovascular disease than respiratory disease, which is primarily attributable to the inhalable particle matters. Our findings provide a good reference to local decision makers for the implementation of further emission control strategies and possible health impacts assessment.
Synthesis of Porous Boron-Doped Carbon Nitride: Adsorption Capacity and Photo-Regeneration Properties
Authors: Guo Xiang , Rao Lei , Wang Peifang , Zhang Lixin , Wang Yuxiong
Published: February 2019

Carbon nitride (CN) with improved adsorption–degradation capacity was synthesized using B2O3 and CN via calcination. The pollutant removal capacity of this B2O3/CN (B-CN) was studied by a powder suspension experiment and added into concrete to evaluate the adsorption and degradation of methylene blue (MB). The characterizations of all samples indicate that B2O3 significantly affects CN, e.g., by increasing the CN specific surface area to 3.6 times the original value, extending visible light adsorption, and narrowing the band gap from 2.56 eV to 2.42 eV. Furthermore, the results show that B-CN composite materials have a higher MB-removal efficiency, with the adsorption capacity reaching 43.11 mg/g, which is about 3.3 times that of pristine CN. The MB adsorption process on B2-CN is mainly via electrostatic attraction and π–π interactions. In addition, B-CN added into concrete also has good performance. After five adsorption–degradation cycles, B-CN and photocatalytic concrete still exhibit a good regenerate ability and excellent stability, which are very important for practical applications.
Nitrogen Removal in Oligotrophic Reservoir Water by a Mixed Aerobic Denitrifying Consortium: Influencing Factors and Immobilization Effects
Authors: Wang Hanyue , Wang Tong , Yang Shangye , Liu Xueqing , Kou Liqing , Huang Tinglin , Wen Gang
Published: February 2019

Nitrogen pollution in reservoirs has received increasing attention in recent years. Although a number of aerobic denitrifying strains have been isolated to remove nitrogen from eutrophic waters, the situation in oligotrophic water environments has not received significant attention. In this study, a mixed aerobic denitrifying consortium screened from reservoir samples was used to remove nitrogen in an oligotrophic denitrification medium and actual oligotrophic source water. The results showed that the consortium removed 75.32% of nitrate (NO3−-N) and 63.11% of the total nitrogen (TN) in oligotrophic reservoir water during a 24-h aerobic cultivation. More initial carbon source was helpful for simultaneous removal of carbon and nitrogen in the reservoir source water. NO3−-N and TN were still reduced by 60.93% and 46.56% at a lower temperature (10 °C), respectively, though the rates were reduced. Moreover, adding phosphorus promoted bacterial growth and increased TN removal efficiency by around 20%. The performance of the immobilized consortium in source water was also explored. After 6 days of immobilization, approximately 25% of TN in the source water could be removed by the carriers, and the effects could last for at least 9 cycles of reuse. These results provide a good reference for the use of aerobic denitrifiers in oligotrophic reservoirs.
An Integrated Framework Combining Multiple Human Activity Features for Land Use Classification
Authors: Ge Panpan , He Jun , Zhang Shuhua , Zhang Liwei , She Jiangfeng
Published: February 2019

Urban land use information is critical to urban planning, but the increasing complexity of urban systems makes the accurate classification of land use extremely challenging. Human activity features extracted from big data have been used for land use classification, and fusing different features can help improve the classification. In this paper, we propose a framework to integrate multiple human activity features for land use classification. Features were fused by constructing a membership matrix reflecting the fuzzy relationship between features and land use types using the fuzzy c-means (FCM) clustering method. The classification results were obtained by the fuzzy comprehensive evaluation (FCE) method, which regards the membership matrix as the fuzzy evaluation matrix. This framework was applied to a case study using taxi trajectory data from Nanjing, and the outflow, inflow, net flow and net flow ratio features were extracted. A series of experiments demonstrated that the proposed framework can effectively fuse different features and increase the accuracy of land use classification. The classification accuracy achieved 0.858 (Kappa = 0.810) when the four features were fused for land use classification.
A Novel Rare Missense Variation of the NOD2 Gene: Evidences of Implication in Crohn’s Disease
Authors: Frade-Proud’Hon-Clerc Sara , Smol Thomas , Frenois Frédéric , Sand Olivier , Vaillant Emmanuel , Dhennin Véronique , Bonnefond Amélie , Froguel Philippe , Fumery Mathurin , Guillon-Dellac Nathalie , Gower-Rousseau Corinne , Vasseur Francis
Published: February 2019

The NOD2 gene, involved in innate immune responses to bacterial peptidoglycan, has been found to be closely associated with Crohn’s Disease (CD), with an Odds Ratio ranging from 3–36. Families with three or more CD-affected members were related to a high frequency of NOD2 gene variations, such as R702W, G908R, and 1007fs, and were reported in the EPIMAD Registry. However, some rare CD multiplex families were described without identification of common NOD2 linked-to-disease variations. In order to identify new genetic variation(s) closely linked with CD, whole exome sequencing was performed on available subjects, comprising four patients in two generations affected with Crohn’s disease without R702W and G908R variation and three unaffected related subjects. A rare and, not yet, reported missense variation of the NOD2 gene, N1010K, was detected and co-segregated across affected patients. In silico evaluation and modelling highlighted evidence for an adverse effect of the N1010K variation with regard to CD. Moreover, cumulative characterization of N1010K and 1007fs as a compound heterozygous state in two, more severe CD family members strongly suggests that N1010K could well be a new risk factor involved in Crohn’s disease genetic susceptibility.
Radiobiological Characterization of Canine Malignant Melanoma Cell Lines with Different Types of Ionizing Radiation and Efficacy Evaluation with Cytotoxic Agents
Authors: Yoshikawa Hiroto , Sunada Shigeaki , Hirakawa Hirokazu , Fujimori Akira , Elmegerhi Suad , Leary Del , Kato Takamitsu A.
Published: February 2019

Canine malignant melanoma (CMM) is a locally and systemically aggressive cancer that shares many biological and clinical characteristics with human mucosal melanoma. Hypofractionated radiation protocols have been used to treat CMM but little is known about its radiation biology. This pilot study is designed to investigate response of CMM cell lines to various ionizing radiations and cytotoxic agents to better understand this canine cancer. Four CMM cell lines were evaluated by clonogenic survival assay under aerobic and hypoxic conditions and parameters such as alpha beta (α/β) ratio, oxygen enhancement ratio (OER), and relative biological effectiveness (RBE) were calculated after 137Cs, 6 megavoltage (MV) photon, or carbon ion irradiation. Six cytotoxic agents (cisplatin, camptothecin, mitomycin C, bleomycin, methtyl methanesulfonate and etoposide) were also assessed for their efficacy. Under aerobic condition with 6 MV photon, the α/β ratio of the four cell lines ranged from 0.3 to >100, indicating a wide variation of cellular sensitivity. The ratio increased under hypoxic condition compared to aerobic condition and this was more dramatic in 137Cs and 6 MV photon treatments. OER of carbon was lower than 137Cs at D10 in 3 of the 4 cell lines. The RBE values generally increased with the increase of LET. Different cell lines showed sensitivity/resistance to different cytotoxic agents. This study revealed that CMM has a wide range of radiosensitivity and that hypoxia can reduce it, indicating that widely used hypofractionated protocols may not be optimal for all CMM patients. Several cytotoxic agents that have never been clinically assessed can improve treatment outcome.
The Anti-Tumor Agent Sodium Selenate Decreases Methylated PP2A, Increases GSK3βY216 Phosphorylation, Including Tau Disease Epitopes and Reduces Neuronal Excitability in SHSY-5Y Neurons
Authors: Habbab Wesal , Aoudé Imad , Palangi Freshteh , Abdulla Sara , Ahmed Tariq
Published: February 2019

Selenium application as sodium selenate was repeatedly shown to have anti-carcinogenic properties by increasing levels of the serine/ threonine protein phosphatase 2A (PP2A) in cancer cells. PP2A has a prominent role in cell development, homeostasis, and in neurons regulates excitability. PP2A, GSK3β and Tau reside together in a complex, which facilitates their interaction and (dys)-function as has been reported for several neurological disorders. In this study we recorded maximum increase in total PP2A at 3 µM sodium selenate in a neuron cell line. In conjunction with these data, whole-cell electrophysiological studies revealed that this concentration had maximum effect on membrane potentials, conductance and currents. Somewhat surprisingly, the catalytically active form, methylated PP2A (mePP2A) was significantly decreased. In close correlation to these data, the phosphorylation state of two substrate proteins, sensitive to PP2A activity, GSK3β and Tau were found to be increased. In summary, our data reveal that sodium selenate enhances PP2A levels, but reduces catalytic activity of PP2A in a dose dependent manner, which fails to reduce Tau and GSK3β phosphorylation under physiological conditions, indicating an alternative route in the rescue of cell pathology in neurological disorders.
Protective Effect of Antrodia cinnamomea Extract against Irradiation-Induced Acute Hepatitis
Authors: Kuo Tsu-Hsiang , Kuo Yueh-Hsiung , Cho Chun-Yu , Yao Chih-Jung , Lai Gi-Ming , Chuang Shuang-En
Published: February 2019

Radiotherapy for treatment of hepatocellular carcinoma causes severe side effects, including acute hepatitis and chronic fibrosis. Complementary and alternative medicine (CAM) has emerged as an important part of integrative medicine in the management of diseases. Antrodia cinnamomea (AC), a valuable medicinal fungus originally found only in Taiwan, has been shown to possess anti-oxidation, vaso-relaxtation, anti-inflammation, anti-hepatitis, and anti-cancer effects. In this paper we evaluate the protective effects of ethanol extract of Antrodia cinnamomea (ACE) against radiotoxicity both in normal liver cell line CL48 and in tumor-bearing mice. In CL48, ACE protects cells by eliminating irradiation-induced reactive oxygen species (ROS) through the induction of Nrf2 and the downstream redox system enzymes. The protective effect of ACE was also demonstrated in tumor-bearing mice by alleviating irradiation-induced acute hepatitis. ACE could also protect mice from CCl4-induced hepatitis. Since both radiation and CCl4 cause free radicals, these results indicate that ACE likely contains active components that protect normal liver cells from free radical attack and can potentially benefit hepatocellular carcinoma (HCC) patients during radiotherapy.
hCMV-Mediated Immune Escape Mechanisms Favor Pathogen Growth and Disturb the Immune Privilege of the Eye
Authors: Spekker-Bosker Katrin , Ufermann Christoph-Martin , Maywald Marco , Zimmermann Albert , Domröse Andreas , Woite Claudia , Däubener Walter , Eller Silvia Kathrin
Published: February 2019

Human retinal pigment epithelial (hRPE) cells are important for the establishment and maintenance of the immune privilege of the eye. They function as target cells for human cytomegalovirus (hCMV), but are able to restrict viral replication. hCMV causes opportunistic posterior uveitis such as retinitis and chorioretinitis. Both mainly occur in severely immunocompromised patients and rarely manifest in immunocompetent individuals. In this study, hRPE cells were infected with hCMV in vitro and activated with proinflammatory cytokines. The enzymatic activities of indoleamine 2,3-dioxygenase-1 (IDO1) and inducible nitric oxide synthase (iNOS) were determined. The antimicrobial capacity of both molecules was analyzed in co-infection experiments using Staphylococcus aureus (S. aureus) and Toxoplasma gondii (T. gondii), causing uveitis in patients. We show that an hCMV infection of hRPE cells blocks IDO1 and iNOS mediated antimicrobial defense mechanisms necessary for the control of S. aureus and T. gondii. hCMV also inhibits immune suppressive effector mechanisms in hRPE. The interferon gamma-induced IDO1 dependent immune regulation was severely blocked, as detected by the loss of T cell inhibition. We conclude that an active hCMV infection in the eye might favor the replication of pathogens causing co-infections in immunosuppressed individuals. An hCMV caused blockade of IDO1 might weaken the eye’s immune privilege and favor the development of post-infectious autoimmune uveitis.
Roles of MicroRNA-34a in Epithelial to Mesenchymal Transition, Competing Endogenous RNA Sponging and Its Therapeutic Potential
Authors: Nie Dongsong , Fu Jiewen , Chen Hanchun , Cheng Jingliang , Fu Junjiang
Published: February 2019

MicroRNA-34a (miR-34a), a tumor suppressor, has been reported to be dysregulated in various human cancers. MiR-34a is involves in certain epithelial-mesenchymal transition (EMT)-associated signal pathways to repress tumorigenesis, cancer progression, and metastasis. Due to the particularity of miR-34 family in tumor-associated EMT, the significance of miR-34a is being increasingly recognized. Competing endogenous RNA (ceRNA) is a novel concept involving mRNA, circular RNA, pseudogene transcript, and long noncoding RNA regulating each other’s expressions using microRNA response elements to compete for the binding of microRNAs. Studies showed that miR-34a is efficient for cancer therapy. Here, we provide an overview of the function of miR-34a in tumor-associated EMT. ceRNA hypothesis plays an important role in miR-34a regulation in EMT, cancer progression, and metastasis. Its potential roles and challenges as a microRNA therapeutic candidate are discussed. As the negative effect on cancer progression, miR-34a should play crucial roles in clinical diagnosis and cancer therapy.