bluuebunny/crossref_metadata_2025 huggingface dataset:
Education and Income Levels are Associated With Energy and Micronutrient Intake
Authors: Cembranel Francieli , Wagner Katia Jakovljevic Pudla , González-Chica David Alejandro , d’Orsi Eleonora
Published: February 2019

Abstract. Introduction: According to the literature, education and income are determinants factors of diet quality and consequently of micronutrient intake. However, this association is still little known among adults who live in middle-income countries. Objective: To estimate energy and micronutrients intake by men and women living in a capital city in southern Brazil, according to education and income levels, and to identify prevalence of inadequate micronutrient intake according also to education and income levels. Method: This is a second wave cross-sectional analysis of a population-based longitudinal study, the EpiFloripa Adultos, including 1,222 individuals of 22–63 years. Data on food consumption were obtained through applying two 24-hour dietary recalls, and the prevalence of inadequate micronutrient intake, following the recommendations of the Institute of Medicine and from the National Research Council. Results: A tendency of increased intake with an increase in income (calcium, vitamins C, E) and education levels (calcium, vitamins A, C, D) was observed for most of the micronutrients analyzed (p<0.05 in all cases); still, a prevalence of inadequacy according to Estimated Average Requirement (EAR) between 85.5–100% in intake of vitamins A, D and E were found for the whole sample. Iron inadequate intake was associated with education level and among women less than 50 years of age (p=0.018). Conclusion: The results showed an influence of the education and income levels on micronutrient intake, point to the need of daily food consumption, of minimally processed and in natura foods (as fruits, vegetables, whole grains, milk and its derivatives) as a means to reduce the encountered inadequacies.
Triggering of the Mw 7.2 Hawaii Earthquake of 4 May 2018 by a Dike Intrusion
Authors: Chen Kejie , Smith Jonathan D. , Avouac Jean‐Philippe , Liu Zhen , Song Y. Tony , Gualandi Adriano
Published: February 2019

A Mw 7.2 earthquake struck the south flank of Kilauea, Hawaii, on 4 May 2018, following a period of volcanic unrest. To investigate its relationship with the stress changes induced by prior tectonic and magmatic activity, we model the coseismic slip distribution, preintrusion deformation, and dike intrusion using geodetic, seismic, and tsunami observations. The décollement beneath the south flank was creeping seaward by ~25 cm/year. Diking started on 20 April and led to fissure eruption on 3 May. The magmatic activity and creep resulted in an onshore U‐shaped zone of stress unloading, fringed by an off‐shore zone of stress buildup that apparently guided the 2018 rupture. It takes only 20 to 35 years at the preintrusion rate to accumulate a moment deficit equivalent to the moment that was released in 2018. This event falls short of balancing the moment budget since the 1975 Mw 7.7 earthquake.
Magma Reservoir Below Laguna del Maule Volcanic Field, Chile, Imaged With Surface‐Wave Tomography
Authors: Wespestad Crystal E. , Thurber Clifford H. , Andersen Nathan L. , Singer Brad S. , Cardona Carlos , Zeng Xiangfang , Bennington Ninfa L. , Keranen Katie , Peterson Dana E. , Cordell Darcy , Unsworth Martyn , Miller Craig , Williams‐Jones Glyn
Published: February 2019

The Laguna del Maule (LdM) volcanic field comprises the greatest concentration of postglacial rhyolite in the Andes and includes the products of ~40 km3 of explosive and effusive eruptions. Recent observations at LdM by interferometric synthetic aperture radar and global navigation satellite system geodesy have revealed inflation at rates exceeding 20 cm/year since 2007, capturing an ongoing period of growth of a potentially large upper crustal magma reservoir. Moreover, magnetotelluric and gravity studies indicate the presence of fluids and/or partial melt in the upper crust near the center of inflation. Petrologic observations imply repeated, rapid extraction of rhyolitic melt from crystal mush stored at depths of 4–6 km during at least the past 26 ka. We utilize multiple types of surface‐wave observations to constrain the location and geometry of low‐velocity domains beneath LdM. We present a three‐dimensional shear‐wave velocity model that delineates a ~450‐km3 shallow magma reservoir ~2 to 8 km below surface with an average melt fraction of ~5%. Interpretation of the seismic tomography in light of existing gravity, magnetotelluric, and geodetic observations supports this model and reveals variations in melt content and a deeper magma system feeding the shallow reservoir in greater detail than any of the geophysical methods alone. Geophysical imaging of the LdM magma system today is consistent with the petrologic inferences of the reservoir structure and growth during the past 20–60 kyr. Taken together with the ongoing unrest, a future rhyolite eruption of at least the scale of those common during the Holocene is a reasonable possibility.
Modeling the Seasonal Cycle of Iron and Carbon Fluxes in the Amundsen Sea Polynya, Antarctica
Authors: St‐Laurent P. , Yager P. L. , Sherrell R. M. , Oliver H. , Dinniman M. S. , Stammerjohn S. E.
Published: February 2019

The Amundsen Sea Polynya (ASP) is distinguished by having the highest net primary production per unit area in the coastal Antarctic. Recent studies have related this high productivity to the presence of fast‐melting ice shelves, but the mechanisms involved are not well understood. In this study we describe the first numerical model of the ASP to represent explicitly the ocean‐ice interactions, nitrogen and iron cycles, and the coastal circulation at high resolution. The study focuses on the seasonal cycle of iron and carbon, and the results are broadly consistent with field observations collected during the summer of 2010–2011. The simulated biogeochemical cycle is strongly controlled by light availability(dictated by sea ice, phytoplankton self‐shading, and variable sunlight). The micronutrient iron exhibits strong seasonality, where scavenging by biogenic particles and remineralization play large compensating roles. Lateral fluxes of iron are also important to the iron budget, and our results confirm the key role played by inputs of dissolved iron from the buoyancy‐driven circulation of melting ice shelf cavities (the “meltwater pump”). The model suggests that westward flowing coastal circulation plays two important roles: it provides additional iron to the ASP and it collects particulate organic matter generated by the bloom and transports it to the west of the ASP. As a result, maps of vertical particulate organic matter fluxes show highest fluxes in shelf regions located west of the productive central ASP. Overall, these model results improve our mechanistic understanding of the ASP bloom, while suggesting testable hypotheses for future field efforts.
It’s Time to Shift Emphasis Away from Code Sharing
Authors: Greene Chad , Thirumalai Kaustubh
Published: February 2019

Building well-documented, citable frameworks for Earth data analysis will encourage scientific replicability by addressing the underlying issues that inhibit code sharing.
Increased 5-HT2C receptor editing predisposes to PTSD-like behaviors and alters BDNF and cytokines signaling
Authors: Règue Mathilde , Poilbout Corinne , Martin Vincent , Franc Bernard , Lanfumey Laurence , Mongeau Raymond
Published: February 2019

Post-traumatic stress disorder (PTSD) is a trauma- and stress-related disorder with dysregulated fear responses and neurobiological impairments, notably at neurotrophic and inflammation levels. Understanding the mechanisms underlying this disease is crucial to develop PTSD models that meet behavioral and neurobiological validity criteria as well as innovative therapeutic approaches. Serotonin 2C receptors (5-HT2CR) are known for their important role in anxiety, and mice having only the fully edited VGV isoform of 5-HT2CR, which thereby overexpressed brain 5-HT2CR, are of special interest to study PTSD predisposition. Innate and conditioned fear-related behaviors were assessed in VGV and wild-type mice. mRNA expression of brain-derived neurotrophic factor (BDNF), tissue-plasminogen activator (tPA), and pro-inflammatory cytokines (IL-6, IL-1β, and calcineurin) were measured by qRT-PCR. The effect of acute and chronic paroxetine was evaluated on both behavior and gene expression. VGV mice displayed greater fear expression, extensive fear extinction deficits, and fear generalization. Paroxetine restored fear extinction in VGV mice when administered acutely and decreased innate fear and fear generalization when administered chronically. In parallel, Bdnf, tPA, and pro-inflammatory cytokines mRNA levels were dysregulated in VGV mice. Bdnf and tPA mRNA expression was decreased in the hippocampus but increased in the amygdala, and chronic paroxetine normalized Bdnf mRNA levels both in the amygdala and the hippocampus. Amygdalar calcineurin mRNA level in VGV mice was also normalized by chronic paroxetine. VGV-transgenic mice displayed behavioral and neurobiological features that could be accessory to the investigation of PTSD and its treatment. Furthermore, these data point out to the role of 5-HT2CR in neuroplasticity and neuroinflammation.
Long non-coding RNA Irm enhances myogenic differentiation by interacting with MEF2D
Authors: Sui Yutong , Han Yu , Zhao Xingyu , Li Dongsong , Li Guangyu
Published: February 2019

Recent studies suggest important roles for long non-coding RNAs as essential regulators of myogenic differentiation. Here, we report that lncRNA Irm is upregulated during myogenesis. Functional analyses show that the overexpression of Irm enhances myogenic differentiation, whereas the inhibition of Irm has completely opposite effects in vitro. Notably, the inhibition of Irm blocks damage-induced muscle regeneration in vivo. Mechanistically, Irm regulates the expression of myogenic genes by directly binding to MEF2D, which in turn promotes the assembly of MyoD/MEF2D on the regulatory elements of target genes. Collectively, we have identified a novel lncRNA that interacts with MEF2D to regulate myogenesis.
Earth system models underestimate carbon fixation by plants in the high latitudes
Authors: Winkler Alexander J. , Myneni Ranga B. , Alexandrov Georgii A. , Brovkin Victor
Published: February 2019

Most Earth system models agree that land will continue to store carbon due to the physiological effects of rising CO2 concentration and climatic changes favoring plant growth in temperature-limited regions. But they largely disagree on the amount of carbon uptake. The historical CO2 increase has resulted in enhanced photosynthetic carbon fixation (Gross Primary Production, GPP), as can be evidenced from atmospheric CO2 concentration and satellite leaf area index measurements. Here, we use leaf area sensitivity to ambient CO2 from the past 36 years of satellite measurements to obtain an Emergent Constraint (EC) estimate of GPP enhancement in the northern high latitudes at two-times the pre-industrial CO2 concentration (3.4 ± 0.2 Pg C yr−1). We derive three independent comparable estimates from CO2 measurements and atmospheric inversions. Our EC estimate is 60% larger than the conventionally used multi-model average (44% higher at the global scale). This suggests that most models largely underestimate photosynthetic carbon fixation and therefore likely overestimate future atmospheric CO2 abundance and ensuing climate change, though not proportionately.
Kondo-like phonon scattering in thermoelectric clathrates
Authors: Ikeda M. S. , Euchner H. , Yan X. , Tomeš P. , Prokofiev A. , Prochaska L. , Lientschnig G. , Svagera R. , Hartmann S. , Gati E. , Lang M. , Paschen S.
Published: February 2019

Crystalline solids are generally known as excellent heat conductors, amorphous materials or glasses as thermal insulators. It has thus come as a surprise that certain crystal structures defy this paradigm. A prominent example are type-I clathrates and other materials with guest-host structures. They sustain low-energy Einstein-like modes in their phonon spectra, but are also prone to various types of disorder and phonon-electron scattering and thus the mechanism responsible for their ultralow thermal conductivities has remained elusive. Our thermodynamic and transport measurements on various clathrate single crystal series and their comparison with ab initio simulations reveal an all phononic Kondo effect as origin. This insight devises design strategies to further suppress the thermal conductivity of clathrates and other related materials classes, with relevance for thermoelectric waste heat recovery and, more generally, phononic applications. It may also trigger theoretical work on strong correlation effects in phonon systems.
Extreme slow growth as alternative strategy to survive deep starvation in bacteria
Authors: Gray Declan A. , Dugar Gaurav , Gamba Pamela , Strahl Henrik , Jonker Martijs J. , Hamoen Leendert W.
Published: February 2019

Bacteria can become dormant or form spores when they are starved for nutrients. Here, we find that non-sporulating Bacillus subtilis cells can survive deep starvation conditions for many months. During this period, cells adopt an almost coccoid shape and become tolerant to antibiotics. Unexpectedly, these cells appear to be metabolically active and show a transcriptome profile very different from that of stationary phase cells. We show that these starved cells are not dormant but are growing and dividing, albeit with a doubling time close to 4 days. Very low nutrient levels, comparable to 10,000-fold diluted lysogeny broth (LB), are sufficient to sustain this growth. This extreme slow growth, which we propose to call ‘oligotrophic growth state’, provides an alternative strategy for B. subtilis to endure nutrient depletion and environmental stresses. Further work is warranted to test whether this state can be found in other bacterial species to survive deep starvation conditions.
Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01
Authors: Gu Yue , Wong Yee Hwa , Liew Chong Wai , Chan Conrad E. Z. , Murali Tanusya M. , Yap Jiawei , Too Chien Tei , Purushotorman Kiren , Hamidinia Maryam , El Sahili Abbas , Goh Angeline T. H. , Teo Rachel Z. C. , Wood Kathryn J. , Hanson Brendon J. , Gascoigne Nicholas R. J. , Lescar Julien , Vathsala Anantharaman , MacAry Paul A.
Published: February 2019

Our understanding of the conformational and electrostatic determinants that underlie targeting of human leukocyte antigens (HLA) by anti-HLA alloantibodies is principally based upon in silico modelling. Here we provide a biochemical/biophysical and functional characterization of a human monoclonal alloantibody specific for a common HLA type, HLA-A*11:01. We present a 2.4 Å resolution map of the binding interface of this antibody on HLA-A*11:01 and compare the structural determinants with those utilized by T-cell receptor (TCR), killer-cell immunoglobulin-like receptor (KIR) and CD8 on the same molecule. These data provide a mechanistic insight into the paratope−epitope relationship between an alloantibody and its target HLA molecule in a biological context where other immune receptors are concomitantly engaged. This has important implications for our interpretation of serologic binding patterns of anti-HLA antibodies in sensitized individuals and thus, for the biology of human alloresponses.
A systematic study on the influence of thermodynamic asymmetry of 5′-ends of siRNA duplexes in relation to their silencing potency
Authors: Lisowiec-Wąchnicka Jolanta , Bartyś Natalia , Pasternak Anna
Published: February 2019

siRNA molecules possess high potential as molecular tools and can be used as effective therapeutics in humans. One of the key steps in the action of these molecules is the choice of antisense strand by the RNA-induced silencing complex (RISC). To explain this process, we verified the theory which states that antisense strand selection is based on the thermodynamically less stable 5′ end of siRNA. Based on the studies presented herein, we observed that for the tested siRNA duplexes, the difference in the thermodynamic stability of the terminal, penultimate and pre-penultimate pairs in the duplex siRNA is not the dominant factor in antisense strand selection. We found that both strands in each tested siRNA molecule are used as an antisense strand. The introduction of modified nucleotides, whose impact on the thermodynamic stability of siRNA duplexes was studied, results in changes in antisense strand selection by the RISC complex. The presence of a modified residue often caused predominant selection of only one antisense strand which is at variance with the theory of siRNA strand bias.
Effects of bleaching-associated mass coral mortality on reef structural complexity across a gradient of local disturbance
Authors: Magel Jennifer M. T. , Burns John H. R. , Gates Ruth D. , Baum Julia K.
Published: February 2019

Structural complexity underpins the ecological functioning of coral reefs. However, rising ocean temperatures and associated coral bleaching threaten the structural integrity of these important ecosystems. Despite the increased frequency of coral bleaching events, few studies to date have examined changes in three-dimensional (3D) reef structural complexity following severe bleaching. The influence of local stressors on reef complexity also remains poorly understood. In the wake of the 2015-2016 El Niño-induced mass coral bleaching event, we quantified the effects of severe heat stress on 3D reef structural complexity across a gradient of local human disturbance. Using Structure-from-Motion photogrammetry we created 3D reconstructions of permanent reef plots and observed substantial declines in reef structural complexity, measured as surface rugosity and terrain ruggedness, and a detectable loss of habitat volume one year after the bleaching event. 3D reef complexity also declined with increasing levels of human disturbance, and with decreasing densities of branching and massive corals. These findings improve our understanding of the effects of local and global stressors on the structural foundation of coral reef ecosystems. In the face of accelerating climate change, mitigating local stressors may increase reef structural complexity, thereby heightening reef resilience to future bleaching events.
A clean fuel cookstove is associated with improved lung function: Effect modification by age and secondhand tobacco smoke exposure
Authors: Mazumder Sarmila , Lee Alison , Dube Brinda , Mehra Divya , Khaing Phue , Taneja Sunita , Yan Beizhan , Chillrud Steven N. , Bhandari Nita , D’Armiento Jeanine M.
Published: February 2019

Household air pollution (HAP) secondary to the burning of solid fuels is a major risk factor for the development of COPD. Our study seeks to examine the impact of a clean cookstove, liquid petroleum gas (LPG), on respiratory outcomes. Women (n = 200) from neighboring Indian communities, one cooking with LPG and one with biomass, were enrolled. Spirometry was performed. Relationships between primary cooking fuel and spirometry measures, as raw values, Global Lung Initiative (GLI) percent predicted (pp), and GLI z-scores, were examined using linear regression. Effect modification by age was explored. Women were young (average age 33.3 years), with low education (median 5.0 years), and the majority had multiple sources of air pollution exposures. Overall, the lung function in both groups was poor [FEV1 z-score median −2.05, IQR (−2.64, −1.41). Biomass was associated with lower FEV1/FVC (raw values −7.0, p = 0.04; GLI pp −7.62, p = 0.05, and z-score −0.86, p = 0.05) and FEF25–75 (GLI pp −25.78, p = 0.05, z-score −1.24, p = 0.05), after adjusting for confounders. Increasing impairment in lung function with age was found among biomass users (p-interaction = 0.01). In conclusion, use of a clean fuel cookstove may improve lung function. These findings have broad implications for research and public policy.
Acoustoelasticity Analysis of Transient Waves for Non-Invasive In Vivo Assessment of Urinary Bladder
Authors: Bayat Mahdi , Adabi Saba , Kumar Viksit , Gregory Adriana , Webb Jeremy , Denis Max , Kim Baehyung , Singh Aparna , Mynderse Lance , Husmann Douglas , Alizad Azra , Fatemi Mostafa
Published: February 2019

A non-invasive method for measurement of the bladder wall nonlinear elastic behavior is presented. The method is based on acoustoelasticity modeling of the elasticity changes in bladder tissue modulus at different volumetric strain levels. At each volume, tissue strain is obtained from the real-time ultrasound images. Using acoustic radiation force, a transient Lamb wave is excited on the bladder wall and instantaneous modulus of shear elasticity is obtained from the 2-D Fourier analysis of the spatial-temporal dispersion maps. Measured elasticity and strain data are then used in an acoustoelasticity formulation to obtain the third order elastic coefficient, referred to as nonlinearity parameter A, and initial resting elasticity μ0. The method was tested in ex vivo porcine bladder samples (N = 9) before and after treatment with formalin. The estimated nonlinearity parameter, A, was significantly higher in the treated samples compared to intact (p < 0.00062). The proposed method was also applied on 16 patients with neurogenic bladders (10 compliant and 6 non-compliant subjects). The estimated nonlinearity parameter A was significantly higher in the non-compliant cases compared to the compliant (p < 0.0293). These preliminary results promise a new method for non-invasive evaluation of the bladder tissue nonlinearity which may serve as a new diagnostic and prognostic biomarker for management of the patients with neurogenic bladders.
Nitrogen–phosphorus-associated metabolic activities during the development of a cyanobacterial bloom revealed by metatranscriptomics
Authors: Lu Jingrang , Zhu Bo , Struewing Ian , Xu Ning , Duan Shunshan
Published: February 2019

The efforts towards reduction of nutrient contamination of surface waters have greatly gained attention to mitigate increasing incidences of harmful cyanobacterial blooms (CyanoHABs), but little attention has been paid on the roles and importance of cyanobacterial N2-fixation and phosphorus (P) scavenging pathways during cyanoHABs. Meta-transcriptomic analyses revealed that expressions of genes involved in N2-fixation (nifDKH) and P-scavenging were significantly upregulated during the bloom compared to pre-bloom in Harsha Lake. The activities of N2-fixation occurred during early summer after a late spring phytoplankton bloom, and were associated with high phosphorus and low nitrogen. The highly active cyanobacterial N2-fixers were dominated by Nostoc and Anabaena. Following the activities of N2-fixation and production of new nitrogen, an early summer Microcystis-dominated bloom, a shift of dominance from Nostoc and Anabaena to Microcystis and an increase of microcystin and saxitoxin occurred. By contrast, P-scavenging activities dominated also by Nostoc and Anabaena were associated with low P and the Microcystis bloom. This information can be used to aid in the understanding the impact that nitrogen and phosphorus have on the early summer CyanoHAB and the functional activities of Nostoc- and Anabaena-dominated or Microcystis-dominated communities, and aid in making management decisions related to harmful algal blooms.
Translational methods to detect asymmetries in temporal and spatial walking metrics in parkinsonian mouse models and human subjects with Parkinson’s disease
Authors: Broom Lauren , Worley Audrey , Gao Fay , Hernandez Laura D. , Ashton Christine E. , Shih Ludy C. , VanderHorst Veronique G.
Published: February 2019

Clinical signs in Parkinson’s disease (PD), including parkinsonian gait, are often asymmetric, but mechanisms underlying gait asymmetries in PD remain poorly understood. A translational toolkit, a set of standardized measures to capture gait asymmetries in relevant mouse models and patients, would greatly facilitate research efforts. We validated approaches to quantify asymmetries in placement and timing of limbs in mouse models of parkinsonism and human PD subjects at speeds that are relevant for human walking. In mice, we applied regression analysis to compare left and right gait metrics within a condition. To compare alternation ratios of left and right limbs before and after induction of parkinsonism, we used circular statistics. Both approaches revealed asymmetries in hind- and forelimb step length in a unilateral PD model, but not in bilateral or control models. In human subjects, a similar regression approach showed a step length asymmetry in the PD but not control group. Sub-analysis of cohorts with predominant postural instability-gait impairment and with predominant tremor revealed asymmetries for step length in both cohorts and for swing time only in the former cohort. This translational approach captures asymmetries of gait in mice and patients. Application revealed striking differences between models, and that spatial and temporal asymmetries may occur independently. This approach will be useful to investigate circuit mechanisms underlying the heterogeneity between models.
Retrograde transport of Akt by a neuronal Rab5-APPL1 endosome
Authors: Goto-Silva Livia , McShane Marisa P. , Salinas Sara , Kalaidzidis Yannis , Schiavo Giampietro , Zerial Marino
Published: February 2019

Long-distance axonal trafficking plays a critical role in neuronal function and transport defects have been linked to neurodegenerative disorders. Various lines of evidence suggest that the small GTPase Rab5 plays a role in neuronal signaling via early endosomal transport. Here, we characterized the motility of Rab5 endosomes in primary cultures of mouse hippocampal pyramidal cells by live-cell imaging and showed that they exhibit bi-directional long-range motility in axons, with a strong bias toward retrograde transport. Characterization of key Rab5 effectors revealed that endogenous Rabankyrin-5, Rabenosyn-5 and APPL1 are all present in axons. Further analysis of APPL1-positive endosomes showed that, similar to Rab5-endosomes, they display more frequent long-range retrograde than anterograde movement, with the endosomal levels of APPL1 correlated with faster retrograde movement. Interestingly, APPL1-endosomes transport the neurotrophin receptor TrkB and mediate retrograde axonal transport of the kinase Akt1. FRET analysis revealed that APPL1 and Akt1 interact in an endocytosis-dependent manner. We conclude that Rab5-APPL1 endosomes exhibit the hallmarks of axonal signaling endosomes to transport Akt1 in hippocampal pyramidal cells.
Higher CXCL16 exodomain is associated with aggressive ovarian cancer and promotes the disease by CXCR6 activation and MMP modulation
Authors: Mir Hina , Kaur Gurpreet , Kapur Neeraj , Bae Sejong , Lillard James W. , Singh Shailesh
Published: February 2019

Ovarian cancer (OvCa) is the leading cause of death from gynecological malignancies. Five-year survival rate of OvCa ranges from 30–92%, depending on the spread of disease at diagnosis. Role of chemokines is well appreciated in cancer, including OvCa. However, their precise role is understudied. Here, we show clinical and biological significance of CXCR6-CXCL16 and ADAM10 in OvCa. Expression of CXCR6 and N-terminal CXCL16 was significantly higher in serous carcinoma tissues compared to endometrioid. OvCa cells (SKOV-3 and OVCAR-3) also showed higher expression of CXCR6 than normal ovarian epithelial cells (IOSE-7576) while CXCL16 was higher in SKOV-3 than IOSE-7576. Furthermore, N-terminal CXCL16 was higher in conditioned media of OvCa cells than IOSE-7576. Compared to OVCAR-3, SKOV-3 cells, which had higher CXCL16, expressed significantly higher transcripts of ADAM10, a protease that cleaves CXCL16. OVCAR-3 cells showed higher CXCR6 specific migration whereas SKOV-3 cells showed more invasion. Difference in invasive potential of these cells was due to modulation of different MMPs after CXCL16 stimulation. Higher CXCR6 expression in serous papillary carcinoma tissues suggests its association with aggressive OvCa. Increased migration-invasion towards CXCL16 implies its role in metastatic spread. Therefore, CXCR6-CXCL16 axis could be used to differentiate between aggressive versus non-aggressive disease and as a target for better prognosis.
Gamma-ray emission from wakefield-accelerated electrons wiggling in a laser field
Authors: Feng Jie , Li Yifei , Wang Jinguang , Li Dazhang , Li Fang , Yan Wenchao , Wang Weimin , Chen Liming
Published: February 2019

Ultra-fast synchrotron radiation emission can arise from the transverse betatron motion of an electron in a laser plasma wakefield, and the radiation spectral peak is limited to tens of keV. Here, we present a new method for achieving high-energy radiation via accelerated electrons wiggling in an additional laser field whose intensity is one order of magnitude higher than that for the self-generated transverse field of the bubble, resulting in an equivalent wiggler strength parameter K increase of approximately twenty times. By calculating synchrotron radiation, we acquired a peak brightness for the case of the laser wiggler field of 1.2 × 1023 ph/s/mrad2/mm2/0.1%BW at 1 MeV. Such a high brilliance and ultra-fast gamma-ray source could be applied to time-resolved probing of dense materials and the production of medical radioisotopes.
Superplastic Forging for Sialon-based Nanocomposite at Ultralow Temperature in the Electric Field
Authors: Luo Junting , Xi Chenyang , Gu Yongfei , Zhang Lili , Zhang Chunxiang , Xue Yahong , Liu Riping
Published: February 2019

The utralow-temperature superplastic forging for sialon-based nanocomposites is reported for the first time. Sialon-based nanocomposites, with an average grain size smaller than 50 nm and 98.5% relative density, were prepared with nano-sized row powders by the spark plasma sintering (SPS) technique at a record ultralow sintering temperature of 1150 °C. An excellent gear is forged at the ultralow deformation temperature of 1200 °C with nanosized grains without any cracking. The maximum strain rate achieved is over 10−1 s−1, and a compression strain is more than 0.9. The practical application for superplastic forming of nitrogen ceramics is much more difficult than that for oxide ceramics because of the high deformation temperature and low strain rates. The present findings present a bright prospect for the near-net-shape superplastic forming of nitrogen ceramics.
Cholinergic Stimulation by Pyridostigmine Bromide Before Myocardial Infarction Prevent Cardiac and Autonomic Dysfunction
Authors: Barboza C. A. , Fukushima A. R. , Carrozzi N. , Machi J. F. , Dourado P. M. M. , Mostarda C. T. , Irigoyen M. C. , Nathanson L. , Morris M. , Caperuto E. C. , Rodrigues B.
Published: February 2019

Inflammatory processes and cardiovascular autonomic imbalance are very relevant characteristic of the enormous dynamic process that is a myocardial infarction (MI). In this sense, some studies are investigating pharmacological therapies using acetylcholinesterase inhibitors, such as pyridostigmine bromide (PYR), aiming to increase parasympathetic tone after MI. Here we hypothesized that the use of PYR before the MI might bring an additional positive effect to the autonomic function, and consequently, in the inflammatory response and cardiac function. The present study aimed to evaluate left ventricular function, baroreflex sensitivity, autonomic modulation, and inflammatory profile in PYR-treated rats previously to MI. Methods: Male Wistar rats (250–300 g) were treated for 60 days with PYR. After treatment, they were submitted to the MI. After the MI, the autonomic and ventricular function were evaluated, as well as the systemic, left ventricle, and adipose tissue inflammatory profile. Results: PYR, performed before MI, prevented HR increase, systolic function impairment, baroreflex sensitivity drop, as well as pulse interval variance, RMSSD, blood pressure and parasympathetic modulation reduction in treated rats compared to untreated rats. Also, this positive functional changes may have been a result of the reduced inflammatory parameters in the left ventricle (IFN-γ, IL-6, and IL-1β), as well as increased IL-10 expression and IL-10/TNF-α ratio in treated animals before MI. Conclusion: Prior treatment with PYR prevents impairment of the autonomic nervous system after MI, which may be associated with the attenuated expression of inflammatory factors and heart dysfunction.
Superresolution microscopy reveals distinct localisation of full length IRSp53 and its I-BAR domain protein within filopodia
Authors: Sudhaharan Thankiah , Hariharan Srivats , Lim John Soon Yew , Liu Jaron Zhongliang , Koon Yen Ling , Wright Graham D. , Chiam Keng Hwee , Ahmed Sohail
Published: February 2019

Superresolution microscopy offers the advantage of imaging biological structures within cells at the nano-scale. Here we apply two superresolution microscopy techniques, specifically 3D structured illumination microscopy (3D-SIM) and direct stochastic optical reconstruction microscopy (dSTORM), a type of single molecule localisation microscopy, to localise IRSp53 protein and its I-BAR domain in relation to F-actin within filopodia. IRSp53 generates dynamic (extending and retracting) filopodia 300 nm wide with a distinct gap between IRSp53 and F-actin. By contrast, protrusions induced by the I-BAR domain alone are non-dynamic measuring between 100–200 nm in width and exhibit a comparatively closer localisation of the I-BAR domain with the F-actin. The data suggest that IRSp53 membrane localisation is spatially segregated to the lateral edges of filopodia, in contrast to the I-BAR domain is uniformly distributed throughout the membranes of protrusions. Modeling of fluorescence recovery after photobleaching (FRAP) data suggests that a greater proportion of I-BAR domain is associated with membranes when compared to full length IRSp53. The significance of this new data relates to the role filopodia play in cell migration and its importance to cancer.
Real time observation of binder jetting printing process using high-speed X-ray imaging
Authors: Parab Niranjan D. , Barnes John E. , Zhao Cang , Cunningham Ross W. , Fezzaa Kamel , Rollett Anthony D. , Sun Tao
Published: February 2019

A high-speed synchrotron X-ray imaging technique was used to investigate the binder jetting additive manufacturing (AM) process. A commercial binder jetting printer with droplet-on-demand ink-jet print-head was used to print single lines on powder beds. The printing process was recorded in real time using high-speed X-ray imaging. The ink-jet droplets showed distinct elongated shape with spherical head, long tail, and three to five trailing satellite droplets. Significant drift was observed between the impact points of main droplet and satellite droplets. The impact of the droplet on the powder bed caused movement and ejection of the powder particles. The depth of disturbance in the powder bed from movement and ejection was defined as interaction depth, which is found to be dependent on the size, shape, and material of the powder particles. For smaller powder particles (diameter less than 10 μm), three consecutive binder droplets were observed to coalesce to form large agglomerates. The observations reported here will facilitate the understanding of underlying physics that govern the binder jetting processes, which will then help in improving the quality of parts manufactured using this AM process.
Seasonal reversible size changes in the braincase and mass of common shrews are flexibly modified by environmental conditions
Authors: Lázaro Javier , Hertel Moritz , Muturi Marion , Dechmann Dina K. N.
Published: February 2019

The growth of the vertebrate skull and brain is usually unidirectional and more or less stops when animals are adult. Red-toothed shrews break this rule. They seasonally shrink and regrow brain and skull size by 20% or more, presumably to save energy when conditions are harsh. The size change is anticipatory of environmental change and occurs in all individuals, but it is unknown whether its extent can be modulated by environmental conditions. We kept shrews under different conditions, monitored seasonal changes in skull size with series of X-rays, and compared them with free ranging animals. We found extensive differences in the pattern of skull size change between experimental groups. Skull size of shrews kept at constant temperature showed a steady decline, while the skull size changes of free ranging shrews and captive individuals exposed to natural temperature regimes were identical. In contrast, body mass never reached the spring values of free ranging shrews in either captive regime. The extent of this adaptive seasonal pattern can thus be flexibly adapted to current environmental conditions. Combining reversible size changes with such strong phenotypic plasticity may allow these small, non-hibernating predators with high metabolic rates to continue being successful in today’s changing environments.
Detection of Volatile Organic Compounds (VOCs) in Urine via Gas Chromatography-Mass Spectrometry QTOF to Differentiate Between Localized and Metastatic Models of Breast Cancer
Authors: Woollam Mark , Teli Meghana , Angarita-Rivera Paula , Liu Shengzhi , Siegel Amanda P. , Yokota Hiroki , Agarwal Mangilal
Published: February 2019

Breast cancer is the most common cancer detected in women and current screening methods for the disease are not sensitive. Volatile organic compounds (VOCs) include endogenous metabolites that provide information about health and disease which might be useful to develop a better screening method for breast cancer. The goal of this study was to classify mice with and without tumors and compare tumors localized to the mammary pad and tumor cells injected into the iliac artery by differences in VOCs in urine. After 4T1.2 tumor cells were injected into BALB/c mice either in the mammary pad or into the iliac artery, urine was collected, VOCs from urine headspace were concentrated by solid phase microextraction and results were analyzed by gas chromatography-mass spectrometry quadrupole time-of-flight. Multivariate and univariate statistical analyses were employed to find potential biomarkers for breast cancer and metastatic breast cancer in mice models. A set of six VOCs classified mice with and without tumors with an area under the receiver operator characteristic (ROC AUC) of 0.98 (95% confidence interval [0.85, 1.00]) via five-fold cross validation. Classification of mice with tumors in the mammary pad and iliac artery was executed utilizing a different set of six VOCs, with a ROC AUC of 0.96 (95% confidence interval [0.75, 1.00]).
The cascading effects of human food on hibernation and cellular aging in free-ranging black bears
Authors: Kirby Rebecca , Johnson Heather E. , Alldredge Mathew W. , Pauli Jonathan N.
Published: February 2019

Human foods have become a pervasive subsidy in many landscapes, and can dramatically alter wildlife behavior, physiology, and demography. While such subsidies can enhance wildlife condition, they can also result in unintended negative consequences on individuals and populations. Seasonal hibernators possess a remarkable suite of adaptations that increase survival and longevity in the face of resource and energetic limitations. Recent work has suggested hibernation may also slow the process of senescence, or cellular aging. We investigated how use of human foods influences hibernation, and subsequently cellular aging, in a large-bodied hibernator, black bears (Ursus americanus). We quantified relative telomere length, a molecular marker for cellular age, and compared lengths in adult female bears longitudinally sampled over multiple seasons. We found that bears that foraged more on human foods hibernated for shorter periods of time. Furthermore, bears that hibernated for shorter periods of time experienced accelerated telomere attrition. Together these results suggest that although hibernation may ameliorate cellular aging, foraging on human food subsidies could counteract this process by shortening hibernation. Our findings highlight how human food subsidies can indirectly influence changes in aging at the molecular level.
The role of serotonin 1B in the representation of outcomes
Authors: Corbit Laura , Kendig Michael , Moul Caroline
Published: February 2019

Disrupted serotonin neurotransmission has been implicated in the etiology of psychopathic traits. Empirical research has found that people with high levels of psychopathic traits have a deficit in reinforcement learning that is thought to be linked with amygdala dysfunction. Altered serotonin neurotransmission provides a plausible explanation for amygdala dysfunction in psychopathic traits and recent research suggests that this may be associated with serotonin 1B (5-HT1B) receptor function. This research used an animal model to test the hypothesis that 5-HT1B receptors are involved in the encoding of the specific features of reinforcing outcomes. An outcome devaluation task was used to test the effect of the systemic administration of a selective 5-HT1B receptor agonist administered before encoding of “action-outcome” associations. Results showed that while administration of a 5-HT1B receptor agonist allowed rats to acquire instrumental responding for food, when the content of that learning was further probed using an outcome devaluation task, performance differed from controls. 5-HT1B agonism impaired learning about the specific sensory qualities of food rewards associated with distinct instrumental responses, required to direct choice performance when the value of one outcome changed. These findings suggest a role for 5-HT1B receptor function in the encoding of the specific features of reinforcing outcomes.
Epigenome-wide association study in peripheral white blood cells involving insulin resistance
Authors: Arpón Ana , Milagro Fermín I. , Ramos-Lopez Omar , Mansego M. Luisa , Santos José Luis , Riezu-Boj José-Ignacio , Martínez J. Alfredo
Published: February 2019

Insulin resistance (IR) is a hallmark of type 2 diabetes, metabolic syndrome and cardiometabolic risk. An epigenetic phenomena such as DNA methylation might be involved in the onset and development of systemic IR. The aim of this study was to explore the genetic DNA methylation levels in peripheral white blood cells with the objective of identifying epigenetic signatures associated with IR measured by the Homeostatic Model Assessment of IR (HOMA-IR) following an epigenome-wide association study approach. DNA methylation levels were assessed using Infinium Methylation Assay (Illumina), and were associated with HOMA-IR values of participants from the Methyl Epigenome Network Association (MENA) project, finding statistical associations for at least 798 CpGs. A stringent statistical analysis revealed that 478 of them showed a differential methylation pattern between individuals with HOMA-IR ≤ 3 and > 3. ROC curves of top four CpGs out of 478 allowed differentiating individuals between both groups (AUC≈0.88). This study demonstrated the association between DNA methylation in some specific CpGs and HOMA-IR values that will help to the understanding and in the development of new strategies for personalized approaches to predict and prevent IR-associated diseases.
Effects of poling and crystallinity on the dielectric properties of Pb(In1/2Nb1/2)O3-Pb(Mg1/3Nb2/3)O3-PbTiO3 at cryogenic temperatures
Authors: Shepley Philippa M. , Stoica Laura A. , Li Yang , Burnell Gavin , Bell Andrew J.
Published: February 2019

The mechanisms underlying the anomalously large, room temperature piezoelectric activity of relaxor-PbTiO3 type single crystals have previously been linked to low temperature relaxations in the piezoelectric and dielectric properties. We investigate the properties of Pb(In1/2Nb1/2)O3-Pb(Mg1/3Nb2/3)O3-PbTiO3 between 10 and 300 K using dielectric permittivity measurements. We compare results on single crystal plates measured in the [001] and [111] directions with a polycrystalline ceramic of the same composition. Poled crystals have very different behaviour to unpoled crystals, whereas the dielectric spectrum of the polycrystalline ceramic changes very little on poling. A large, frequency dependent dielectric relaxation is seen in the poled [001] crystal around 100 K. The relaxation is much less prominent in the [111] cut crystal, and is not present in the polycrystalline ceramic. The unique presence of the large relaxation in poled, [001] oriented crystals indicates that the phenomenon is not due their relaxor nature alone. We propose that heterophase dynamics such as the motion of phase domain boundaries are responsible for both the anomalous electromechanical and dielectric behaviour.
Discovery of Anion Insertion Electrochemistry in Layered Hydroxide Nanomaterials
Authors: Young Matthias J. , Kiryutina Tatyana , Bedford Nicholas M. , Woehl Taylor J. , Segre Carlo U.
Published: February 2019

Electrode materials which undergo anion insertion are a void in the materials innovation landscape and a missing link to energy efficient electrochemical desalination. In recent years layered hydroxides (LHs) have been studied for a range of electrochemical applications, but to date have not been considered as electrode materials for anion insertion electrochemistry. Here, we show reversible anion insertion in a LH for the first time using Co and Co-V layer hydroxides. By pairing in situ synchrotron and quartz crystal microbalance measurements with a computational unified electrochemical band-diagram description, we reveal a previously undescribed anion-insertion mechanism occurring in Co and Co-V LHs. This proof of concept study demonstrates reversible electrochemical anion insertion in LHs without significant material optimization. These results coupled with our foundational understanding of anion insertion electrochemistry establishes LHs as a materials platform for anion insertion electrochemistry with the potential for future application to electrochemical desalination.
Comparisons between multi-component myelin water fraction, T1w/T2w ratio, and diffusion tensor imaging measures in healthy human brain structures
Authors: Uddin Md. Nasir , Figley Teresa D. , Solar Kevin G. , Shatil Anwar S. , Figley Chase R.
Published: February 2019

Various MRI techniques, including myelin water imaging, T1w/T2w ratio mapping and diffusion-based imaging can be used to characterize tissue microstructure. However, surprisingly few studies have examined the degree to which these MRI measures are related within and between various brain regions. Therefore, whole-brain MRI scans were acquired from 31 neurologically-healthy participants to empirically measure and compare myelin water fraction (MWF), T1w/T2w ratio, fractional anisotropy (FA), axial diffusivity (AD), radial diffusivity (RD) and mean diffusivity (MD) in 25 bilateral (10 grey matter; 15 white matter) regions-of-interest (ROIs). Except for RD vs. T1w/T2w, MD vs. T1w/T2w, moderately significant to highly significant correlations (p < 0.001) were found between each of the other measures across all 25 brain structures [T1w/T2w vs. MWF (Pearson r = 0.33, Spearman ρ = 0.31), FA vs. MWF (r = 0.73, ρ = 0.75), FA vs. T1w/T2w (r = 0.25, ρ = 0.22), MD vs. AD (r = 0.57, ρ = 0.58), MD vs. RD (r = 0.64, ρ = 0.61), AD vs. MWF (r = 0.43, ρ = 0.36), RD vs. MWF (r = −0.49, ρ = −0.62), MD vs. MWF (r = −0.22, ρ = −0.29), RD vs. FA (r = −0.62, ρ = −0.75) and MD vs. FA (r = −0.22, ρ = −0.18)]. However, while all six MRI measures were correlated with each other across all structures, there were large intra-ROI and inter-ROI differences (i.e., with no one measure consistently producing the highest or lowest values). This suggests that each quantitative MRI measure provides unique, and potentially complimentary, information about underlying brain tissues – with each metric offering unique sensitivity/specificity tradeoffs to different microstructural properties (e.g., myelin content, tissue density, etc.).