bluuebunny/crossref_metadata_2025 huggingface dataset:
Political Ideologies of CEOs
Authors: Chin M. K. , Hambrick Donald C. , Treviño Linda K.
Published: April 2013

This article examines the influence on organizational outcomes of CEOs’ political ideology, specifically political conservatism vs. liberalism. We propose that CEOs’ political ideologies will influence their firms’ corporate social responsibility (CSR) practices, hypothesizing that (1) liberal CEOs will emphasize CSR more than will conservative CEOs; (2) the association between a CEO’s political ideology and CSR will be amplified by a CEO’s relative power; and (3) liberal CEOs will emphasize CSR even when recent financial performance is low, whereas conservative CEOs will pursue CSR initiatives only as performance allows. We test our ideas with a sample of 249 CEOs, measuring their ideologies by coding their political donations over the ten years prior to their becoming CEOs. Results indicate that the political ideologies of CEOs are manifested in their firms’ CSR profiles. Compared with conservative CEOs, liberal CEOs exhibit greater advances in CSR; the influence of CEOs’ political liberalism on CSR is amplified when they have more power; and liberal CEOs’ CSR initiatives are less contingent on recent performance than are those of conservative CEOs. In a corroborative exploration, we find that CEOs’ political ideologies are significantly related to their corporate political action committee (PAC) allocations, indicating that this largely unexplored executive attribute might be more widely consequential.
Vocational Psychology, Offenders and Ex-Offenders, and Social Justice
Authors: McWhirter Ellen Hawley
Published: April 2013

The vocational needs of offenders and ex-offenders have received little attention in the counseling psychology literature. The authors of this special issue have called attention to the importance of and possibilities associated with the development and implementation of vocational interventions for such populations. This reaction discusses the merits of the articles in this special issue, and proposes that in addition to focusing on developing effective interventions for offenders and ex-offenders, we must interrogate the U.S. prison enterprise with respect to goals, outcomes, and ultimately, social justice.
Effectiveness of Prediabetes Nutrition Shared Medical Appointments
Authors: Cole Renee E. , Boyer Kathleen M. , Spanbauer Sean M. , Sprague Denise , Bingham Mona
Published: April 2013

Purpose

The purpose of this study is to evaluate the effectiveness of a nutrition-based shared medical appointment (SMA) intervention in the treatment of prediabetes compared to the individualized counseling standard of care.

Methods

A randomized controlled trial design comparing health outcomes in patients with prediabetes attending either an individualized counseling (control group) or three 90-minute nutrition SMA (intervention group) sessions. Demographic, anthropometric (weight and body mass index), clinical (blood pressure), and biochemical (lipid profile, fasting blood sugar, glycated hemoglobin, albumin-to-creatinine ratio) measures were obtained from all participants at baseline, at 3 months, and at 1 year.

Results

Ninety-four participants were randomized into the 2 study groups with a 69% completion rate at 1 year (n = 34 SMA, n = 31 control). The average participant was Caucasian (64%), male (54%), 58.3 ± 9.6 years, had a BMI of 30.8 ± 4.9 kg/m2 (obese), and fasting blood glucose of 109 ± 9.5 mg/dL. The SMA and control participants lost a mean of 6.6 pounds and 3.6 pound, respectively; neither group met the 5% modest weight loss expected. The SMA and control group experienced a mean drop in fasting blood glucose of 6 mg/dL.

Conclusions

As demands on health care providers continue to rise, finding innovative ways to manage the patient load while providing quality health care is increasingly important. SMA health outcomes were equivalent to individual counseling outcomes, while increasing the provider’s productivity by treating 6 to 8 people with prediabetes in 90 minutes compared to 1 patient in 60 minutes.
Freedom and identity
Authors: Kaul Volker
Published: April 2013

As show the partly violent clashes between liberal secularists and Islamists in the aftermath of the Arab Spring, the two factions certainly defend two diametrically opposite political points of view. For liberals, politics finds its ultimate justification in the protection of individual freedom. For Islamists, only the application of the moral code and religious law codified in the shariah can justify politics. Contrary to what is sustained by a theory of situated agency, there is no easy and definite reconciliation between the two positions. And this depends precisely upon the fact that both political models are based upon the very same idealist conception of the individual, namely the assumption that we, as persons, have a free will and are not determined by the law of causality. Paradoxical as it might sound, it is our freedom that gives rise to the problem of identity and lends force to the Islamist argumentation. If freedom as such cannot bring about practical reason and also liberals recognize that the ultimate source of normativity is identity, there is a point in the Islamist and, more general, communitarian claim that we are not free to choose our identity. In order that identity does the normative work it is supposed to do, it must be given and not chosen. What remains, however, unclear in the communitarian picture is how the norms of our community can come to constitute our will without a process of active identification. If we cannot identify voluntarily with our community’s norms, then only emotional attachment to our community can explain identification and the normative grip communitarian norms have upon us. Yet, attachment is conditioned by the effective satisfaction of our psychological and physical needs. The problem is that our need for freedom and liberty can become overshadowed by our more immediate needs based, for example, upon resentment and revenge and that today makes Muslims in particular to be so hostile towards liberal ideas. I suggest that conciliatory trust-building measures can help to surmount the anger, fear, mistrust and suspicion Muslims feel vis-à-vis the West and that are at the origin of today’s conflict between freedom and identity in the Muslim world.
Understanding the Attitude-Behavior Gap for Renewable Energy Systems Using Behavioral Reasoning Theory
Authors: Claudy Marius C. , Peterson Mark , O’Driscoll Aidan
Published: April 2013

Consumer adoption of renewable energies is an important step towards less carbon-intensive and more sustainable energy systems. But despite growing ecological awareness and articulated preferences for green products, renewable energies face slow rates of diffusion in consumer markets. This has been hard to explain given consumers’ favorability to the concept of products that lower one’s impact on the natural environment. This study uses data from 254 homeowners in Ireland to investigate the psychological process of adopting a renewable energy system – solar energy panels. Applying Behavioral Reasoning Theory (BRT), this research examines a proposed model in which reasons both for and against adopting solar panels mediate the relationship between consumers’ attitudes, values and adoption intentions. Results suggest the model is generally supported with both reasons for adoption and reasons against adoption having countervailing influences in the psychological processing of adoption intentions. These findings suggest that researchers and marketers should include mediating constructs, such as (i) reasons for adoption, (ii) reasons against adoption, and (iii) attitudes toward a technology when attempting to explain how consumers think about the adoption of renewable energy systems.
Investigation of possible epistatic interactions between GRIA2 and GRIA4 variants on clinical outcomes in patients with major depressive disorder
Authors: Chiesa Alberto , Lia Loredana , Lia Claudia , Lee Soo-Jung , Han Changsu , Patkar Ashwin A , Pae Chi-Un , Serretti Alessandro
Published: April 2013

Objectives

To investigate the effects of glutamate receptor, ionotropic, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) 2 ( GRIA2) rs4260586 and glutamate receptor, ionotropic, AMPA 4 ( GRIA4) rs10736648 single nucleotide polymorphisms (SNPs) on response to antidepressants in Korean patients with major depressive disorder (MDD), and to ascertain whether epistatic interactions might exist between these SNPs.

Methods

In this retrospective analysis, patients were assessed at hospital admission and discharge using the Montgomery–Åsberg depression rating scale (MADRS). A multiple regression model was employed to investigate the effects of the two SNP variants on clinical/sociodemographic outcomes relating to MDD.

Results

Out of 145 Korean patients, the presence of both GRIA2 rs4260586 and GRIA4 rs10736648 polymorphisms had no significant association with MADRS improvement scores or other clinical/sociodemographic variables.

Conclusions

These data potentially suggest a lack of epistatic interaction between GRIA2 and GRIA4 variants, regarding clinical outcomes in patients with MDD. The study was limited by small sample size, use of different antidepressants and incomplete coverage of genes under investigation. Future research should include larger patient samples treated with different antidepressants, analysis of different SNPs and/or investigation of different gene–gene interactions within the glutamatergic system.
Catechol-O-methyltransferase Val158Met polymorphism and risk of autism spectrum disorders
Authors: Guo Tianyou , Wang Weiqian , Liu Bing , Chen Hong , Yang Chuang
Published: April 2013

Objective

Autism spectrum disorders (ASD) are a family of childhood-onset neurodevelopmental disorders with complex genetic mechanisms underlying their aetiology. The aim of this case–control study was to evaluate the effect of the catechol-O-methyltransferase ( COMT) gene Val158Met polymorphism on ASD risk in a Chinese Han population.

Methods

The COMT gene Val158Met polymorphism was detected by polymerase chain reaction–restriction fragment length polymorphism in children (≤18 years old) with ASD and healthy control subjects.

Results

The frequency of the Val158/Val158 genotype in children with ASD (22/186; 11.8%) was significantly lower than in controls (38/186; 20.4%). When stratifying by select-item scores on the Autism Diagnostic Interview-Revised protocol, it was found that children with ‘current overactivity’ and ‘ever overactivity’ had a significantly lower frequency of the Val158/Val158 genotype than those without. There were no significant associations between the COMT gene Val158Met polymorphism and ASD subtypes.

Conclusions

The COMT gene Val158Met polymorphism may be a biomarker for phenotypic variation in ASD, but these preliminary findings remain tentative, pending replication in larger, independent samples.
The Moderating Effects of Ethnicity and Employment Type on Insurance Coverage
Authors: Nguyen Duy , Choi Sunha , Park So Young
Published: April 2013

Despite nearly universal insurance coverage for older Americans over the age of 65, the preretirement age cohort is susceptible to gaps in coverage. Related to the Patient Protection and Affordable Care Act (ACA), this study investigated heterogeneity in insurance status for preretirement Asian immigrants by examining the interacting effects of Asian ethnicity and employment type, which is a major factor that determines an individual’s insurance status in the U.S. Data from the 2009 California Health Interview Survey, which included 1,024 Asians between the ages of 50 and 64, were analyzed. Our findings indicate significant moderating effects of employment type and Asian ethnicity. However, regardless of employment type, Koreans had the highest rate of being uninsured. To effectively reach the ACA’s goal of reducing the number of uninsured individuals, targeted interventions specific to Asian subgroups are essential.
Amelioration of hepatotoxicity induced by aflatoxin using citrus fruit oil in broilers (Gallus domesticus)
Authors: Kumar D Senthil , Rao Suguna , Satyanarayana ML , Kumar PG Pradeep , Anitha N
Published: April 2013

This study was conducted to evaluate the efficacy of citrus fruit oil (CFO; 2.5 g kg−1) on the clinicopathological changes in broilers fed with diets containing 1 ppm of aflatoxin (AF). A total of 160 Ross 308 broiler chicks of 1-day-old were procured from a commercial hatchery, divided randomly on 7th day of age into four groups with two replicates of 20 birds each and fed with basal diet (group A), basal diet + CFO (group B), basal diet + AF (group C) and CFO + basal diet + AF (group D). The gross and histopathological changes in the liver, kidney, spleen, thymus and bursa of Fabricius were investigated and relative organ weights were calculated. Slight to moderate hydropic degeneration, fatty change with the formation of cyst in some cases, periportal necrosis, infiltration of heterophils and mononuclear cells and bile duct hyperplasia were observed in chicks fed with 1 ppm AF-containing diet. The addition of CFO to AF-containing diet moderately decreased the magnitude and severity of lesions (hydropic degeneration and bile duct hyperplasia) in the liver. The supplementation of CFO to the basal diet did not produce any adverse effects in birds.
The New Zealand/Aotearoa Baby-Friendly Hospital Initiative Implementation Journey
Authors: Martis Ruth , Stufkens Julie
Published: April 2013

The Baby-Friendly Hospital Initiative (BFHI) was launched by the World Health Organization and United Nations International Children’s Emergency Fund in 1991 following the Innocenti Declaration of 1990, of which New Zealand/Aotearoa was a signatory. After a number of unsuccessful attempts to implement BFHI by breastfeeding advocates, the New Zealand Breastfeeding Authority (NZBA) was established in 1999. Successful BFHI implementation was achieved through a number of factors, including the establishment of a national body (NZBA) with oversight of BFHI facility implementation and auditing, Māori and consumer participation at all levels, the recertification requirements for midwives of breastfeeding education by the Midwifery Council, and maternity facilities having paid BFHI coordinators. Once the NZBA was established, BFHI accreditation of maternity facilities had a rapid rise from 0% in 2000 to 96.1% in 2011 (74 of 77 facilities). Data collected over this period shows a 28.8-percentage-point increase of exclusive breastfeeding at discharge from maternity facilitates between 2001 (55.6%) and 2011 (84.4%). Future strategies focus on having all relevant community providers achieve Baby-Friendly Community Initiative accreditation and on the NZBA’s lobbying the government for initiatives that support, promote, and protect breastfeeding.
Monogamy Lite
Authors: Wilkins Amy C. , Dalessandro Cristen
Published: April 2013

Studies of collegiate sexuality have not examined infidelity. Using in-depth peer interviews with college students, our article investigates the meanings and practices of “monogamy” and “cheating” for college women. College women use ideas about age, class, and gender to construct collegiate sexuality as a kind of “monogamy lite” exempt from the “rules” of adult sexuality. Many have cheated themselves. Simultaneously, they define “real” relationships as exclusive and condemn “cheaters” as bad people. We employ an intersectional analysis to analyze these discrepancies, arguing that the multiple meanings women use reconcile contradictions between expectations for women’s sexuality and expectations about collegiate behavior, allowing women to sustain a commitment to relationships while also participating in collegiate sexual culture. Moreover, by providing a socially legible, gender-appropriate way to end unwanted relationships, these meanings allow women to use cheating to solve dilemmas in their intimate lives. In this case, college women use middle-class ideas about the transition to adulthood to resist gendered imperatives.
The weakest link? Product market strategies, skill and pay in the hotel industry
Authors: Lloyd Caroline , Warhurst Chris , Dutton Eli
Published: April 2013

There is a widely held assumption that product market strategies, skill and pay are linked. Supportive evidence is typically drawn from manufacturing and using quantitative analyses. Emergent research of the link in services is ambivalent and has methodological limitations. This article addresses this weakness. It compares the skills and pay of room attendants in upper and mid-market hotels using qualitative research. It finds that the link is weak, even decoupled. The findings suggest a reconceptualization is needed of the link in services and that interventions other than product market re-positioning are needed to deliver higher skills and better pay.
Synthesis and characterization of poly(arylene ether ketone) copolymers with pendant carboxylic acid groups for antifouling ultrafiltration membrane
Authors: Ren Jiannan , Liu Yang , Wang Yan , Wang Qinhong , Wang Guibin
Published: April 2013

This work focused on the synthesis of a series of poly(arylene ether ketone) random copolymers with various controlled pendant carboxylic acid groups (PEAK-COOH) by direct copolymerization method and the preparation of antifouling ultrafiltration membranes via the conventional immersion precipitation phase inversion method. The morphologies of the PEAK-COOH membranes were investigated by scanning electron microscopy. In addition, the surface hydrophilicity and charged property of the PEAK-COOH membranes were studied by water contact angle and membrane potential measurements, and the results indicated that the introduction of COOH on the polymer chains of membrane materials was really an effective way to negatively charge the membrane and enhance the hydrophilicity. The pure water flux and protein solution permeation through the prepared membranes were increased with the increase in the content of carboxylic acid groups. The cycle ultrafiltration experiments for protein solution revealed that nonspecific protein adsorption, especially irreversible protein adsorption, for the PEAK-COOH membranes, was significantly reduced, suggesting superior antifouling performance.
Customized innovation policies and the regions: digital content services and intelligent machinery in Finland
Authors: Sotarauta Markku , Kosonen Kati-Jasmin
Published: April 2013

This paper poses the question of whether innovation policies are customized to meet the varying needs of different regions and industries. The research questions are: (a) are the investigated innovation initiatives context sensitive and customized to the prevailing innovation problems and, hence, to what extent do they focus on overcoming specific bottlenecks in the particular regional innovation system and address system failures hampering innovation; (b) have innovation policies aiming to support specific industries recognized the differences and, if yes, have they been customized accordingly? The paper discusses emerging forms of local/regional innovation policy using, as cases in point, Finland and especially two different industries (intelligent machinery and digital content services) in three different kinds of regional innovation system. The empirical research is based on data gathered (a) through 40 interviews with Finnish innovation policy makers and (b) through 91 structured interviews with firm representatives; (c) in addition, interview data from another study with 53 national-level innovation policy makers are exploited. The empirical analysis shows that, in spite of a shared understanding about the generic principles of the innovation policy and the investigated local/regional policy, initiatives are clearly customized to serve the current challenges of the specific regions and industries.
Utilization of Home Telemonitoring in Patients 75 Years of Age and Over with Complex Heart Failure
Authors: Lemay Geneviève , Azad Nahid , Struthers Christine
Published: April 2013

We conducted a chart review on all patients who had received home telemonitoring after an admission for heart failure at the University of Ottawa Heart Institute. During a 5 year period (2005–2009) a total of 645 patients had home monitoring. A total of 594 patients met the inclusion criteria for the study and were divided into two groups: Group 1 (<75 years of age) contained 350 patients and Group 2 (≥75 years of age) contained 244 patients. There was no significant difference between the groups in the mean duration of follow-up by the telemonitoring team: it was 126.5 days in Group 1 and 125.4 days in Group 2 ( P = 0.89). There were no significant differences between the groups in the number of times that titration of diuretic medications occurred, the number of times that titration of cardiac medications occurred, the number of interventions for abnormal vital signs or the number of times that patients were called by the telemonitoring staff. Emergency room visits, hospitalizations and the number of deaths were also not different between two groups. Thus in the telemonitoring programme, the pattern of usage by older patients was similar to that of the younger ones. Based on the present study, the elderly do not require more resources nor do they require them for longer.
Threshold concepts and student engagement: Revisiting pedagogical content knowledge
Authors: Zepke Nick
Published: April 2013

This article revisits the notion that to facilitate quality learning requires teachers in higher education to have pedagogical content knowledge. It constructs pedagogical content knowledge as a teaching and learning space that brings content and pedagogy together. On the content knowledge side, it suggests that threshold concepts, akin to a portal that opens up a new and previously inaccessible way of thinking about a subject, are useful in quality learning. On the pedagogy side, it employs student engagement as a useful proxy for identifying what happens in a learning environment to achieve quality learning. This article asks what fresh insights might this particular conceptualization of pedagogical content knowledge afford teacher education and teacher development in achieving quality learning in higher education. After outlining characteristics of threshold concepts and student engagement, it brings together the contributions these concepts make to pedagogical content knowledge before detailing some fresh insights afforded by the synthesis.
Diabetic complications in pregnancy: is resveratrol a solution?
Authors: Singh Chandra K , Kumar Ambrish , LaVoie Holly A , DiPette Donald J , Singh Ugra S
Published: April 2013

Diabetes is a metabolic disorder that, during pregnancy, may affect fetal development. Fetal outcome depends on the type of diabetes present, the concentration of blood glucose and the extent of fetal exposure to elevated or frequently fluctuating glucose concentrations. The result of some diabetic pregnancies will be embryonic developmental abnormalities, a condition referred to as diabetic embryopathy. Tight glycemic control in type 1 diabetes during pregnancy using insulin therapy together with folic acid supplementation are partially able to prevent diabetic embryopathy; however, the protection is not complete and additional interventions are needed. Resveratrol, a polyphenol found largely in the skins of red grapes, is known to have antidiabetic action and is in clinical trials for the treatment of diabetes, insulin resistance, obesity and metabolic syndrome. Studies of resveratrol in a rodent model of diabetic embryopathy reveal that it significantly improves the embryonic outcome in terms of diminishing developmental abnormalities. Improvements in maternal and embryonic outcomes observed in rodent models may arise from resveratrol's antioxidative potential, antidiabetic action and antidyslipidemic nature. Whether resveratrol will have similar actions in human diabetic pregnancy is unknown. Here, we review the potential therapeutic use of resveratrol in diabetes and diabetic pregnancy.
[d-Ala2,d-Leu5]-enkephalin (DADLE) and morphine-induced postconditioning by inhibition of mitochondrial permeability transition pore, in human myocardium
Authors: Fuardo Marinella , Lemoine Sandrine , Coco Claudia Lo , Hanouz Jean Luc , Massetti Massimo
Published: April 2013

The aim of the study was to examine the cardioprotective effect of morphine and Delta 2 opioid d-Ala2-Leu5 enkephalin (DADLE) administered, at early reoxygenation, in isolated human myocardium exposed to hypoxia–reoxygenation. Then, we tested the involvement of mitochondrial permeability transition pore in morphine and DADLE-induced postconditioning. Human right atrial trabeculae were obtained during cardiac surgery (coronary artery bypass and aortic valve replacement). Isometrically contracting isolated human right atrial trabeculae were exposed to 30-min hypoxia and 60-min reoxygenation (control group). In treatment groups, morphine 0.5 μmol, DADLE 10 nmol, DADLE 50 nmol and DADLE 100 nmol were administered during the first 15 min of reoxygenation. In two additional groups, morphine and DADLE 100 nmol were administered in the presence of atractyloside 50 μmol, the mitochondrial permeability transition pore opener. The force of contraction at the end of 60-min reoxygenation period (FoC60 expressed as % of baseline) was compared (mean ± standard deviation) between the groups by an analysis of variance. Morphine (FoC60: 81 ± 9% of baseline), DADLE 50 nmol (FoC60: 76 ± 11% of baseline) and DADLE 100 nmol (FoC60: 81 ± 4% of baseline) increased significantly ( P < 0.001) the FoC60 as compared with the control group (FoC60: 53 ± 3% of baseline). DADLE 10 nmol did not modify the FoC60 (50 ± 9% of baseline; P = 0.60 versus control group). The enhanced recovery of FoC60 induced by morphine and DADLE 100 nmol were abolished in the presence of atractyloside (FoC60: respectively 57 ± 6% and 44 ± 7% of baseline; P < 0.001). In conclusion, the administration of morphine and DADLE, in early reoxygenation period, protected human myocardium, in vitro, against hypoxia–reoxygenation injury, at least in part, by the inhibition of mitochondrial permeability transition pore opening.
Dual Campus High School
Authors: Mombourquette Carmen P.
Published: April 2013

September 2010 witnessed the opening of the first complete dual campus high school in Alberta. Catholic Central High School, which had been in existence since 1967 in one building, now offered courses to students on two campuses. The “dual campus” philosophy was adopted so as to ensure maximum program flexibility for students. The philosophy, however, was destined to affect student engagement and staff efficacy as the change in organizational structure, campus locations, and course availability was dramatic. Changing school organizational structure also had the potential of affecting student achievement. A mixed-methods study utilizing engagement surveys, efficacy scales, and interviews with students and teachers was used to ascertain the degree of impact. The results of the study showed that minimal impact occurred to levels of student engagement, minor negative impact to staff efficacy, and a slight increase to student achievement results.
Public Memorializing in the Stadium
Authors: Butterworth Michael L.
Published: April 2013

Among the purported virtues of sport, whether through participation or viewership, is its capacity to foster community. In the years since September 11, 2001, the institutions associated with the production of mediated sport have constituted community rhetorically through nationalistic and militaristic rituals and ceremonies. Such ceremonies played a prominent role in the public memorialization of 9/11 on its 10th anniversary in 2011. Although it is surely the case that some communal healing is possible through mediated sport and its ceremonies, this essay argues that the centrality of this theme constitutes an illusion of democracy. As a consequence, these mediated sport productions shaped a public memory of 9/11 that diminishes active citizenship and deflects attention away from the consequences of American actions since the terrorist attacks.
Good Choices, Poor Choices
Authors: Waterman Alan S. , Schwartz Seth J. , Hardy Sam A. , Kim Su Yeong , Lee Richard M. , Armenta Brian E. , Whitbourne Susan Krauss , Zamboanga Byron L. , Brown Elissa J. , Williams Michelle K. , Agocha V. Bede
Published: April 2013

Research indicates making identity commitments on the part of emerging adults is associated with a wide range of psychosocial benefits. Data from a large research collaborative were used to evaluate hypotheses drawn from eudaimonic identity theory that the benefits of commitment are attributable to the quality of the commitments held. Findings from a study with 9,650 students attending 30 colleges and universities replicated previous research indicating the benefits of identity commitments with respect to subjective well-being, psychological well-being, self-esteem, an internal locus of control; and reduced likelihood of symptoms of general anxiety, social anxiety, and depression. However, when a measure of the quality of identity commitments was added to the analyses, results indicated that commitment quality accounted almost entirely for the associations of identity commitments with psychosocial functioning. Identity commitments of low quality were found to be associated with psychological costs rather than benefits. Implications for helping emerging adults distinguish better identity choices are discussed.
Adhesion to osteopontin in the bone marrow niche regulates lymphoblastic leukemia cell dormancy
Authors: Boyerinas Benjamin , Zafrir Maya , Yesilkanal Ali E. , Price Trevor T. , Hyjek Elizabeth M. , Sipkins Dorothy A.
Published: April 2013

Key Points

Stromal OPN anchors leukemia cells in prodormancy BM niches. Inhibiting this interaction leads dormant cells to proliferate, sensitizing them to chemotherapy.
Some generalized difference statistically convergent sequence spaces in 2-normed space
Authors: Başarır Metin , Konca Şükran , Kara Emrah Evren
Published: April 2013

In this paper, we define a new generalized difference matrix

B

(
m
)

n

and introduce some

B

(
m
)

n

-difference statistically convergent sequence spaces in a real linear 2-normed space. We also investigate some topological properties of these spaces.

MSC:40A05, 46A45, 46E30.
Characterization of adipose-derived stromal/stem cells from the twitcher mouse model of krabbe disease
Authors: Zhang Xiujuan , Semon Julie A , Zhang Shijia , Strong Amy L , Scruggs Brittni A , Gimble Jeffrey M , Bunnell Bruce A
Published: April 2013

Background

Krabbe disease, also known as globoid cell leukodystrophy, is an autosomal recessive neurodegenerative disease caused by the genetic deficiency of galactocerebrosidase (GALC), a lysosomal enzyme responsible for the degradation of several glycosphingolipids like psychosine and galactosylceramide. In order to investigate whether GALC deficiency in Krabbe disease affects adipose-derived stromal/stem cell (ASC) properties and if the ASCs could be used as a source of autologous stem cell therapy for patients with Krabbe disease, ASCs isolated from subcutaneous adipose tissue of Twitcher mice (a murine model of Krabbe disease) and their normal wild type littermates were cultured, expanded, and characterized for their cell morphology, surface antigen expression, osteogenic and adipogenic differentiation, colony forming units, growth kinetics, and immune regulatory capacities in vitro.

Results

ASCs from Twitcher mice (TwiASCs), when compared to ASCs from normal mice (WtASCs), have a reduced osteogenic differentiation potential, have less self-replicating and proliferative capacity, although they have the same fibroblast morphologies and cell sizes. However, surprisingly, the TwiASCs demonstrated similar immune-suppressive capacities as their counterparts WtASCs did when they were transwell co-cultured with macrophages in vitro.

Conclusion

This study reveals that Twitcher ASCs exhibit differences in the biologic potential when compared to their counterparts from normal mice. The changes in Twitcher ASCs may be influenced by the GALC deficiency in Twitcher mice. Nevertheless, none of the changes preclude the use of the TwiASCs for autologous applications.
Stop Antibiotics on guidance of Procalcitonin Study (SAPS): a randomised prospective multicenter investigator-initiated trial to analyse whether daily measurements of procalcitonin versus a standard-of-care approach can safely shorten antibiotic duration in intensive care unit patients - calculated sample size: 1816 patients
Authors: Assink-de Jong Evelien , de Lange Dylan W , van Oers Jos A , Nijsten Maarten W , Twisk Jos W , Beishuizen Albertus
Published: April 2013

Background

Unnecessary long-term use of broad-spectrum antibiotics is linked to the emergence and selection of resistant bacteria, prolonged hospitalisation and increased costs. Several clinical trials indicate that the biomarker procalcitonin (PCT) can guide antibiotic therapy. Some of these trials have shown a promising reduction in the number of antibiotic prescriptions, duration of antibiotic therapy and even length of stay in the ICU, although their size and selection criteria limit their external validity. The objectives of the Stop Antibiotics on guidance of Procalcitonin Study (SAPS) are to evaluate whether daily PCT can improve “real-life” antibiotic use in Dutch ICU’s by reduction of the duration of antibiotic treatment without an increase of recurrent infections and mortality.

Methods/Design

Multicenter randomised controlled intervention trial. Powered for superiority of the primary efficacy endpoint and non-inferiority on the primary safety endpoints (non-inferiority margin is set on 8%).

Inclusion criteria: (1) ICU-patients aged ≥18 years and (2) receiving antibiotics for a presumed or proven infection and (3) signed informed consent. Exclusion criteria: (1) patients who require prolonged antibiotic therapy, (2) suffer from Mycobacterium tuberculosis, (3) cystic fibrosis, (4) viral or parasitic infections and (5) those that are severely immunocompromised or (6) moribund.

The intervention consists solely of an advice to discontinue antibiotic treatment in case PCT has decreased by more than 80% of its peak level (relative stopping threshold) or decrease below a value of 0.5 ng/ml (absolute stopping threshold).

The study hypothesis is that PCT-guided therapy is non-inferior to standard care based on implemented guidelines and local expertise, whilst reducing antibiotic usage. Computerised 1:1 randomisation will allocate 908 patients per arm. Arm 1: standard of care. Arm 2: procalcitonin-guided therapy. The primary efficacy endpoint is consumption of antibiotics expressed as the defined daily dosage and duration of antibiotic therapy expressed in days of therapy. This trial is designed to shorten antibiotics safely, therefore the primary safety endpoint is mortality measured at 28 day and 1 year.

Discussion

This will be the largest procalcitonin-guided antibiotic intervention trial in ICU setting thus far. Currently 1600 of the planned 1816 patients are randomised (November 2012). The first interim analysis has passed without any safety or futility issues.

Trial registration

Trial registration number at http://www.clinicaltrials.gov: Id. Nr. NCT01139489, at http://www.trialregister.nl: Id.nr. NTR1861.
The acceptability and feasibility of an intercultural birth center in the highlands of Chiapas, Mexico
Authors: Tucker Kathryn , Ochoa Hector , Garcia Rosario , Sievwright Kirsty , Chambliss Amy , Baker Margaret C
Published: April 2013

Background

An intercultural birthing house was established in the Highlands of Chiapas, Mexico, as an intervention to reduce maternal mortality among indigenous women. This birth center, known locally as the Casa Materna, is a place where women can come to give birth with their traditional birth attendant. However, three months after opening, no woman had used the birthing house.

Methods

This study reports on the knowledge, attitudes and practices related to childbirth and use of the Casa Materna from the perspective of the health workers, traditional birth attendants and the program’s target population. Structured interviews, in-depth interviews and focus group discussions were conducted with participants from each of these groups. Data was searched for emerging themes and coded.

Results and conclusions

Findings show that the potential success of this program is jeopardized by lack of transport and a strong cultural preference for home births. The paper highlights the importance of community participation in planning and implementing such an intervention and of establishing trust and mutual respect among key actors. Recommendations are provided for moving forward the maternal health agenda of indigenous women in Chiapas.
Negative regulation of NF-κB signaling in T lymphocytes by the ubiquitin-specific protease USP34
Authors: Poalas Konstantinos , Hatchi Emeline M , Cordeiro Nelia , Dubois Sonia M , Leclair Héloïse M , Leveau Claire , Alexia Catherine , Gavard Julie , Vazquez Aimé , Bidère Nicolas
Published: April 2013

Background

NF-κB is a master gene regulator involved in plethora of biological processes, including lymphocyte activation and proliferation. Reversible ubiquitinylation of key adaptors is required to convey the optimal activation of NF-κB. However the deubiquitinylases (DUBs), which catalyze the removal of these post-translational modifications and participate to reset the system to basal level following T-Cell receptor (TCR) engagement continue to be elucidated.

Findings

Here, we performed an unbiased siRNA library screen targeting the DUBs encoded by the human genome to uncover new regulators of TCR-mediated NF-κB activation. We present evidence that knockdown of Ubiquitin-Specific Protease 34 (USP34) selectively enhanced NF-κB activation driven by TCR engagement, similarly to siRNA against the well-characterized DUB cylindromatosis (CYLD). From a molecular standpoint, USP34 silencing spared upstream signaling but led to a more pronounced degradation of the NF-κB inhibitor IκBα, and culminated with an increased DNA binding activity of the transcription factor.

Conclusions

Collectively, our data unveils USP34 as a new player involved in the fine-tuning of NF-κB upon TCR stimulation.
The Lnk/SH2B adaptor provides a fail-safe mechanism to establish the Insulin receptor-Chico interaction
Authors: Almudi Isabel , Poernbacher Ingrid , Hafen Ernst , Stocker Hugo
Published: April 2013

Background

Insulin/insulin-like growth factor signalling (IIS) has been described as one of the major pathways involved in growth control and homeostasis in multicellular organisms. Whereas its core components are well established, less is known about the molecular functions of IIS regulators. The adaptor molecule Lnk/SH2B has been implicated in IIS but the mechanism by which it promotes IIS activity has remained enigmatic.

Results

In this study, we analyse genetic and physical interactions among InR, Chico and Lnk in Drosophila tissues. FRET analysis reveals in vivo binding between all three molecules. Genetically, Lnk acts upstream of Chico. We demonstrate that Chico’s plasma membrane localisation is ensured by both its PH domain and by the interaction with Lnk. Furthermore, Lnk is able to recruit an intracellular InR fragment to the membrane.

Conclusions

Thus, by acting as a scaffolding molecule that ensures InR and Chico enrichment at the membrane, Lnk provides a fail-safe mechanism for IIS activation.
Genome-wide analysis of three-way interplay among gene expression, cancer cell invasion and anti-cancer compound sensitivity
Authors: Hsu Yi-Chiung , Chen Hsuan-Yu , Yuan Shinsheng , Yu Sung-Liang , Lin Chia-Hung , Wu Guani , Yang Pan-Chyr , Li Ker-Chau
Published: April 2013

Background

Chemosensitivity and tumor metastasis are two primary issues in cancer management. Cancer cells often exhibit a wide range of sensitivity to anti-cancer compounds. To gain insight on the genetic mechanism of drug sensitivity, one powerful approach is to employ the panel of 60 human cancer cell lines developed by the National Cancer Institute (NCI). Cancer cells also show a broad range of invasion ability. However, a genome-wide portrait on the contributing molecular factors to invasion heterogeneity is lacking.

Methods

Our lab performed an invasion assay on the NCI-60 panel. We identified invasion-associated (IA) genes by correlating our invasion profiling data with the Affymetrix gene expression data on NCI-60. We then employed the recently released chemosensitivity data of 99 anti-cancer drugs of known mechanism to investigate the gene-drug correlation, focusing on the IA genes. Afterwards, we collected data from four independent drug-testing experiments to validate our findings on compound response prediction. Finally, we obtained published clinical and molecular data from two recent adjuvant chemotherapy cohorts, one on lung cancer and one on breast cancer, to test the performance of our gene signature for patient outcome prediction.

Results

First, we found 633 IA genes from the invasion-gene expression correlation study. Then, for each of the 99 drugs, we obtained a subset of IA genes whose expression levels correlated with drug-sensitivity profiles. We identified a set of eight genes (EGFR, ITGA3, MYLK, RAI14, AHNAK, GLS, IL32 and NNMT) showing significant gene-drug correlation with paclitaxel, docetaxel, erlotinib, everolimus and dasatinib. This eight-gene signature (derived from NCI-60) for chemosensitivity prediction was validated by a total of 107 independent drug tests on 78 tumor cell lines, most of which were outside of the NCI-60 panel. The eight-gene signature predicted relapse-free survival for the lung and breast cancer patients (log-rank P = 0.0263; 0.00021). Multivariate Cox regression yielded a hazard ratio of our signature of 5.33 (95% CI = 1.76 to 16.1) and 1.81 (95% CI = 1.19 to 2.76) respectively. The eight-gene signature features the cancer hallmark epidermal growth factor receptor (EGFR) and genes involved in cell adhesion, migration, invasion, tumor growth and progression.

Conclusions

Our study sheds light on the intricate three-way interplay among gene expression, invasion and compound-sensitivity. We report the finding of a unique signature that predicts chemotherapy survival for both lung and breast cancer. Augmenting the NCI-60 model with in vitro characterization of important phenotype-like invasion potential is a cost-effective approach to power the genomic chemosensitivity analysis.
Consistent high concentration of the viral microRNA BART17 in plasma samples from nasopharyngeal carcinoma patients - evidence of non-exosomal transport
Authors: Gourzones Claire , Ferrand François-Régis , Amiel Corinne , Vérillaud Benjamin , Barat Ana , Guérin Maryse , Gattolliat Charles-Henry , Gelin Aurore , Klibi Jihène , Chaaben Arij Ben , Schneider Véronique , Guemira Fethi , Guigay Joël , Lang Philippe , Jimenez-Pailhes Anne-Sophie , Busson Pierre
Published: April 2013

Background

Because latent Epstein Barr (EBV)-infection is a specific characteristic of malignant nasopharyngeal carcinoma (NPC), various molecules of viral origin are obvious candidate biomarkers in this disease. In a previous study, we could show in a few clinical samples that it was possible to detect a category of EBV microRNAs called miR-BARTs in the plasma of at least a fraction of NPC patients. The first aim of the present study was to investigate the status of circulating miR-BART17-5p (one of the miR-BARTs hereafter called miR-BART17) and EBV DNA in a larger series of NPC plasma samples. The second aim was to determine whether or not circulating miR-BART17 was carried by plasma exosomes.

Patients and methods

Plasma samples were collected from 26 NPC patients and 10 control donors, including 9 patients with non-NPC Head and Neck squamous cell carcinoma and one healthy EBV carrier. Concentrations of miR-BART17 and two cellular microRNAs (hsa-miR-16 and -146a) were assessed by real-time quantitative PCR with spike-in normalization and absolute quantification. In addition, for 2 patients, exosome distributions of miR-BART17 and miR-16 were investigated following plasma lipoprotein fractionation by isopycnic density gradient ultrcentrifugation.

Results

The miR-BART17 was significantly more abundant in plasma samples from NPC patients compared to non-NPC donors. Above a threshold of 506 copies/mL, detection of miR-BART17 was highly specific for NPC patients (ROC curve analysis: AUC=0.87 with true positive rate = 0.77, false positive rate = 0.10). In this relatively small series, the concentration of plasma miR-BART17 and the plasma EBV DNA load were not correlated. When plasma samples were fractionated, miR-BART17 co-purified with a protein-rich fraction but not with exosomes.

Conclusions

Detection of high concentrations of plasma miR-BART17 is consistent in NPC patients. This parameter is, at least in part, independent of the viral DNA load. Circulating miR-BART17 does not co-purify with exosomes.
The utilization and management of plant resources in rural areas of the Limpopo Province, South Africa
Authors: Rasethe Marula T , Semenya Sebua S , Potgieter Martin J , Maroyi Alfred
Published: April 2013

Background

Most rural people in the Limpopo Province depend on plant resources to meet their livelihood needs. However, there is insufficient recorded information regarding their use and management. The current study therefore was carried out in selected villages of the Limpopo Province, to close this knowledge gap.

Methods

Information was collected from 60 people residing in two villages, using a semi-structured questionnaire, supplemented with field observations.

Results

A total of 47 wild plant species (95% indigenous and 5% exotics) from 27 families, mostly from the Fabaceae (17%), Anacardiaceae (9%), and Combretaceae (9%) were documented. These species were used primarily for firewood (40%), food (36%) and medicine (29%). Significantly used species included Sclerocarya birrea (85%), Combretum kraussii (35%) and Harpephyllum caffrum (35%). Local traditional rules and regulations including taboos, social beliefs and fines are in place to aid in the management of communal resources. However, a significant number (67%) of participants mentioned that they were not pleased with these rules and regulations.

Conclusion

The current study concluded that plant resources still play an important role in the surveyed rural areas of the Limpopo Province. Furthermore, for sustainable utilization and long-term conservation of plants in these areas the government should assist communities in the management of their plant resources.