bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Improvement of Medical Optical Fibre Pressure and Temperature Sensors
Authors: Poeggel Sven , Duraibabu Dineshbabu , Kalli Kyriacos , Leen Gabriel , Dooly Gerard , Lewis Elfed , Kelly Jimmy , Munroe Maria
Published: July 2015

This investigation describes a detailed analysis of the fabrication and testing of optical fibre pressure and temperature sensors (OFPTS). The optical sensor of this research is based on an extrinsic Fabry–Perot interferometer (EFPI) with integrated fibre Bragg grating (FBG) for simultaneous pressure and temperature measurements. The sensor is fabricated exclusively in glass and with a small diameter of 0.2 mm, making it suitable for volume-restricted bio-medical applications. Diaphragm shrinking techniques based on polishing, hydrofluoric (HF) acid and femtosecond (FS) laser micro-machining are described and analysed. The presented sensors were examined carefully and demonstrated a pressure sensitivity in the range of \(s_p\) = 2–10 \(\frac{\text{nm}}{\text{kPa}}\) and a resolution of better than \(\Delta P\) = 10 Pa protect (0.1 cm H\(_2\)O). A static pressure test in 38 cmH\(_2\)O shows no drift of the sensor in a six-day period. Additionally, a dynamic pressure analysis demonstrated that the OFPTS never exceeded a drift of more than 130 Pa (1.3 cm H\(_2\)O) in a 12-h measurement, carried out in a cardiovascular simulator. The temperature sensitivity is given by \(k=10.7\) \(\frac{\text{pm}}{\text{K}}\), which results in a temperature resolution of better than \(\Delta T\) = 0.1 K. Since the temperature sensing element is placed close to the pressure sensing element, the pressure sensor is insensitive to temperature changes.
Fractionation and Characterization of High Aspect Ratio Gold Nanorods Using Asymmetric-Flow Field Flow Fractionation and Single Particle Inductively Coupled Plasma Mass Spectrometry
Authors: Nguyen Thao , Liu Jingyu , Hackley Vincent
Published: July 2015

Gold nanorods (GNRs) are of particular interest for biomedical applications due to their unique size-dependent longitudinal surface plasmon resonance band in the visible to near-infrared. Purified GNRs are essential for the advancement of technologies based on these materials. Used in concert, asymmetric-flow field flow fractionation (A4F) and single particle inductively coupled mass spectrometry (spICP-MS) provide unique advantages for fractionating and analyzing the typically complex mixtures produced by common synthetic procedures. A4F fractions collected at specific elution times were analyzed off-line by spICP-MS. The individual particle masses were obtained by conversion of the ICP-MS pulse intensity for each detected particle event, using a defined calibration procedure. Size distributions were then derived by transforming particle mass to length assuming a fixed diameter. The resulting particle lengths correlated closely with ex situ transmission electron microscopy. In contrast to our previously reported observations on the fractionation of low-aspect ratio (AR) GNRs (AR < 4), under optimal A4F separation conditions the results for high-AR GNRs of fixed diameter (≈20 nm) suggest normal, rather than steric, mode elution (i.e., shorter rods with lower AR generally elute first). The relatively narrow populations in late eluting fractions suggest the method can be used to collect and analyze specific length fractions; it is feasible that A4F could be appropriately modified for industrial scale purification of GNRs.
Faster Together: Collective Quantum Search
Authors: Ellinas Demosthenes , Konstandakis Christos
Published: July 2015

Joining independent quantum searches provides novel collective modes of quantum search (merging) by utilizing the algorithm’s underlying algebraic structure. If n quantum searches, each targeting a single item, join the domains of their classical oracle functions and sum their Hilbert spaces (merging), instead of acting independently (concatenation), then they achieve a reduction of the search complexity by factor O(√n).
Transformation toward a Secure and Precaution-Oriented Energy System with the Guiding Concept of Resilience—Implementation of Low-Exergy Solutions in Northwestern Germany
Authors: Brand Urte , Von Gleich Arnim
Published: July 2015

Climate changes, incidents like nuclear disasters, and associated political objectives call for significant changes to the current energy system. Despite these far-reaching transformation processes, within the intended changes security of supply and precautions against the possible consequences of climate change must be ensured. Consequently, the question arises how to direct energy systems. In this context the processes of guiding orientations with the help of the guiding concept of “resilient systems” and feasible and addressee-oriented guiding design principles can be an option to provide guidance in transformation processes. However, it is questionable whether and how such processes are effective in the long term and if they are able to give direction by doing so. Within the framework of empirical studies of a regional guiding orientation process for the energy system of Northwestern Germany, the long-term effectiveness of the process and its spread resilient guiding design principles of “low-exergy solutions” and “climate-adapted and energy-efficient refrigeration” has been confirmed. Such effectiveness requires the implementation of a four-phase guiding orientation process which takes content-related and process-related effectiveness factors into account. Therefore, the study shows how regional energy systems can be designed toward the major challenges of ensuring security and precaution.
Improved Adaptive Droop Control Design for Optimal Power Sharing in VSC-MTDC Integrating Wind Farms
Authors: Ran Xiaohong , Miao Shihong , Wu Yingjie
Published: July 2015

With the advance of insulated gate bipolar transistor (IGBT) converters, Multi-Terminal DC (MTDC) based on the voltage-source converter (VSC) has developed rapidly in renewable and electric power systems. To reduce the copper loss of large capacity and long distance DC transmission line, an improved droop control design based on optimal power sharing in VSC-MTDC integrating offshore wind farm is proposed. The proposed approach provided a calculation method for power-voltage droop coefficients under two different scenarios either considering local load or not. The available headroom of each converter station was considered as a converter outage, to participate in the power adjustment according to their ability. A four-terminal MTDC model system including two large scale wind farms was set up in PSCAD/EMTDC. Then, the proposed control strategy was verified through simulation under the various conditions, including wind speed variation, rectifier outage and inverter outage, and a three-phase short-circuit of the converter.
Organic Food: A Comparative Study of the Effect of Tomato Cultivars and Cultivation Conditions on the Physico-Chemical Properties
Authors: Araujo Jacqueline , Telhado Samuel
Published: July 2015

The objective of this review was to present an update of the currently managed studies on the characterization physical, chemical, and sensory analysis of several tomato cultivars. This review has indicated the importance of farming system and genotype on sensory and biochemical characteristics. It is necessary to use selected genotypes responding positively to organic farming in terms of sensory, biochemical characteristics and productivity aspects and to evaluate systems over more than one year of sampling.
One, Two, Three: Polycomb Proteins Hit All Dimensions of Gene Regulation
Authors: Del Prete Stefania , Mikulski Pawel , Schubert Daniel , Gaudin Valérie
Published: July 2015

Polycomb group (PcG) proteins contribute to the formation and maintenance of a specific repressive chromatin state that prevents the expression of genes in a particular space and time. Polycomb repressive complexes (PRCs) consist of several PcG proteins with specific regulatory or catalytic properties. PRCs are recruited to thousands of target genes, and various recruitment factors, including DNA-binding proteins and non-coding RNAs, are involved in the targeting. PcG proteins contribute to a multitude of biological processes by altering chromatin features at different scales. PcG proteins mediate both biochemical modifications of histone tails and biophysical modifications (e.g., chromatin fiber compaction and three-dimensional (3D) chromatin conformation). Here, we review the role of PcG proteins in nuclear architecture, describing their impact on the structure of the chromatin fiber, on chromatin interactions, and on the spatial organization of the genome in nuclei. Although little is known about the role of plant PcG proteins in nuclear organization, much is known in the animal field, and we highlight similarities and differences in the roles of PcG proteins in 3D gene regulation in plants and animals.
Chromatin Dynamics in the Regulation of CFTR Expression
Authors: Gosalia Nehal , Harris Ann
Published: July 2015

The contribution of chromatin dynamics to the regulation of human disease-associated loci such as the cystic fibrosis transmembrane conductance regulator (CFTR) gene has been the focus of intensive experimentation for many years. Recent technological advances in the analysis of transcriptional mechanisms across the entire human genome have greatly facilitated these studies. In this review we describe the complex machinery of tissue-specific regulation of CFTR expression, and put earlier observations in context by incorporating them into datasets generated by the most recent genomics methods. Though the gene promoter is required for CFTR expression, cell-type specific regulatory elements are located elsewhere in the gene and in flanking intergenic regions. Probably within its own topological domain established by the architectural proteins CTCF and cohesin, the CFTR locus utilizes chromatin dynamics to remodel nucleosomes, recruit cell-selective transcription factors, and activate intronic enhancers. These cis-acting elements are then brought to the gene promoter by chromatin looping mechanisms, which establish long-range interactions across the locus. Despite its complexity, the CFTR locus provides a paradigm for elucidating the critical role of chromatin dynamics in the transcription of individual human genes.
Heritability and Genome-Wide Association Studies for Hair Color in a Dutch Twin Family Based Sample
Authors: Lin Bochao , Mbarek Hamdi , Willemsen Gonneke , Dolan Conor , Fedko Iryna , Abdellaoui Abdel , De Geus Eco , Boomsma Dorret , Hottenga Jouke-Jan
Published: July 2015

Hair color is one of the most visible and heritable traits in humans. Here, we estimated heritability by structural equation modeling (N = 20,142), and performed a genome wide association (GWA) analysis (N = 7091) and a GCTA study (N = 3340) on hair color within a large cohort of twins, their parents and siblings from the Netherlands Twin Register (NTR). Self-reported hair color was analyzed as five binary phenotypes, namely “blond versus non-blond”, “red versus non-red”, “brown versus non-brown”, “black versus non-black”, and “light versus dark”. The broad-sense heritability of hair color was estimated between 73% and 99% and the genetic component included non-additive genetic variance. Assortative mating for hair color was significant, except for red and black hair color. From GCTA analyses, at most 24.6% of the additive genetic variance in hair color was explained by 1000G well-imputed SNPs. Genome-wide association analysis for each hair color showed that SNPs in the MC1R region were significantly associated with red, brown and black hair, and also with light versus dark hair color. Five other known genes (HERC2, TPCN2, SLC24A4, IRF4, and KITLG) gave genome-wide significant hits for blond, brown and light versus dark hair color. We did not find and replicate any new loci for hair color.
Snow Depth Retrieval with UAS Using Photogrammetric Techniques
Authors: Vander Jagt Benjamin , Lucieer Arko , Wallace Luke , Turner Darren , Durand Michael
Published: July 2015

Alpine areas pose challenges for many existing remote sensing methods for snow depth retrieval, thus leading to uncertainty in water forecasting and budgeting. Herein, we present the results of a field campaign conducted in Tasmania, Australia in 2013 from which estimates of snow depth were derived using a low-cost photogrammetric approach on-board a micro unmanned aircraft system (UAS). Using commercial off-the-shelf (COTS) sensors mounted on a multi-rotor UAS and photogrammetric image processing techniques, the results demonstrate that snow depth can be accurately retrieved by differencing two surface models corresponding to the snow-free and snow-covered scenes, respectively. In addition to accurate snow depth retrieval, we show that high-resolution (50 cm) spatially continuous snow depth maps can be created using this methodology. Two types of photogrammetric bundle adjustment (BA) routines are implemented in this study to determine the optimal estimates of sensor position and orientation, in addition to 3D scene information; conventional BA (which relies on measured ground control points) and direct BA (which does not require ground control points). Error sources that affect the accuracy of the BA and subsequent snow depth reconstruction are discussed. The results indicate the UAS is capable of providing high-resolution and high-accuracy (<10 cm) estimates of snow depth over a small alpine area (~0.7 ha) with significant snow accumulation (depths greater than one meter) at a fraction of the cost of full-size aerial survey approaches. The RMSE of estimated snow depths using the conventional BA approach is 9.6 cm, whereas the direct BA is characterized by larger error, with an RMSE of 18.4 cm. If a simple affine transformation is applied to the point cloud derived from the direct BA, the overall RMSE is reduced to 8.8 cm RMSE.
Effect of Neem (Azadirachta indica) on the Survival of Escherichia coli O157:H7 in Dairy Manure
Authors: Ravva Subbarao , Korn Anna
Published: July 2015

Escherichia coli O157:H7 (EcO157) shed in cattle manure can survive for extended periods of time and intervention strategies to control this pathogen at the source are critical as produce crops are often grown in proximity to animal raising operations. This study evaluated whether neem (Azadirachta indica), known for its antimicrobial and insecticidal properties, can be used to amend manure to control EcO157. The influence of neem materials (leaf, bark, and oil) on the survival of an apple juice outbreak strain of EcO157 in dairy manure was monitored. Neem leaf and bark supplements eliminated the pathogen in less than 10 d with a D-value (days for 90% elimination) of 1.3 d. In contrast, nearly 4 log CFU EcO157/g remained after 10 d in neem-free manure control. The ethyl acetate extractable fraction of neem leaves was inhibitory to the growth of EcO157 in LB broth. Azadirachtin, a neem product with insect antifeedant properties, failed to inhibit EcO157. Application of inexpensive neem supplements to control pathogens in manure and possibly in produce fields may be an option for controlling the transfer of foodborne pathogens from farm to fork.
Analysis of the Consumption of Caffeinated Energy Drinks among Polish Adolescents
Authors: Nowak Dariusz , Jasionowski Artur
Published: July 2015

Background: Energy drinks (EDs) are extremely popular among adults and adolescents. Regular intake of EDs may lead to an overdose of caffeine, loss of bone mass, overweight, hypertension and, in older age, osteoporosis and cardiovascular diseases. Some people mix EDs with alcohol, which adversely affects their health. The objective of this study was to analyze the consumption of EDs by adolescents. Methods: The study consisted of a questionnaire surveying amounts of drinks, preferences and product awareness among younger consumers. The study was carried out in junior and senior high schools in Poland (n = 2629). Results: EDs were consumed by 67% of students (quite frequently by 16%). Students who practiced sports were more willing to drink EDs. Also, boys drank them more often than girls. When selecting a particular ED, young people looked at the taste, price and effect. Most respondents consumed one ED (250 mL) daily, although there were individuals consuming two or more drinks daily. Most respondents knew the ingredients of EDs, and 24% admitted to mixing EDs with alcohol. Conclusions: EDs are extremely popular among adolescents. Young people drinking EDs every day are potentially at risk of taking an overdose of caffeine.
PM2.5 in Urban and Rural Nursery Schools in Upper Silesia, Poland: Trace Elements Analysis
Authors: Mainka Anna , Zajusz-Zubek Elwira , Kaczmarek Konrad
Published: July 2015

Indoor air quality (IAQ) in nursery schools is an emerging public health challenge. Particular attention should be paid to younger children, because they are more vulnerable to air pollution than older children. Among air pollutants, fine particulate matter (PM2.5) is of the greatest interest mainly due to its strong association with acute and chronic effects on children’s health. In this paper, we present concentrations of PM2.5 and the composition of its trace elements at naturally ventilated nursery schools located in the area of Gliwice, Poland. The nursery schools were selected to characterize areas with different degrees of urbanization and traffic densities during the winter and spring seasons. The results indicate there is a problem with elevated concentrations of PM2.5 inside the examined classrooms. The children’s exposure to trace elements was different based on localization and season. PM2.5 concentration and its trace element composition have been studied using correlation coefficients between the different trace elements, the enrichment factor (EF) and principal component analysis (PCA). PCA allowed the identification of the three components: anthropogenic and geogenic sources (37.2%), soil dust contaminated by sewage sludge dumping (18.6%) and vehicular emissions (19.5%).
Structural Disorder within Paramyxoviral Nucleoproteins and Phosphoproteins in Their Free and Bound Forms: From Predictions to Experimental Assessment
Authors: Habchi Johnny , Longhi Sonia
Published: July 2015

We herein review available computational and experimental data pointing to the abundance of structural disorder within the nucleoprotein (N) and phosphoprotein (P) from three paramyxoviruses, namely the measles (MeV), Nipah (NiV) and Hendra (HeV) viruses. We provide a detailed molecular description of the mechanisms governing the disorder-to-order transition that the intrinsically disordered C-terminal domain (NTAIL) of their N proteins undergoes upon binding to the C-terminal X domain (PXD) of the homologous P proteins. We also show that NTAIL–PXD complexes are “fuzzy”, i.e., they possess a significant residual disorder, and discuss the possible functional significance of this fuzziness. Finally, we emphasize the relevance of N–P interactions involving intrinsically disordered proteins as promising targets for new antiviral approaches, and end up summarizing the general functional advantages of disorder for viruses.
Plant MYB Transcription Factors: Their Role in Drought Response Mechanisms
Authors: Baldoni Elena , Genga Annamaria , Cominelli Eleonora
Published: July 2015

Water scarcity is one of the major causes of poor plant performance and limited crop yields worldwide and it is the single most common cause of severe food shortage in developing countries. Several molecular networks involved in stress perception, signal transduction and stress responses in plants have been elucidated so far. Transcription factors are major players in water stress signaling. In recent years, different MYB transcription factors, mainly in Arabidopsis thaliana (L.) Heynh. but also in some crops, have been characterized for their involvement in drought response. For some of them there is evidence supporting a specific role in response to water stress, such as the regulation of stomatal movement, the control of suberin and cuticular waxes synthesis and the regulation of flower development. Moreover, some of these genes have also been characterized for their involvement in other abiotic or biotic stresses, an important feature considering that in nature, plants are often simultaneously subjected to multiple rather than single environmental perturbations. This review summarizes recent studies highlighting the role of the MYB family of transcription factors in the adaptive responses to drought stress. The practical application value of MYBs in crop improvement, such as stress tolerance engineering, is also discussed.
Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
Authors: Luo Weiwei , Liu Chuanshu , Cao Xiaojuan , Huang Songqian , Wang Weimin , Wang Yeke
Published: July 2015

RNA sequencing and short-read assembly was utilized to produce a transcriptome of ovarian tissues from three-year-old diploid and tetraploid loaches (Misgurnus anguillicaudatus). A total of 28,369 unigenes were obtained, comprising 10,546 unigenes with length longer than 1000 bp. More than 73% of the unigenes were annotated through sequence comparison with databases. The RNA-seq data revealed that 2253 genes were differentially expressed between diploid and tetraploid loaches, including 1263 up-regulated and 990 down-regulated genes in tetraploid loach. Some differentially expressed genes, such as vitellogenin (Vtg), gonadotropin releasing hormone receptor type A (GnRHRA), steroidogenic acute regulatory protein (StAR), mitogen-activated protein kinase 14a (MAPK14a), ATP synthase subunit alpha (atp5a), and synaptonemal complex protein 1 (Scp1), were involved in regulation of cell proliferation, division, gene transcription, ovarian development and energy metabolism, suggesting that these genes were related to egg diameter of the loach. Results of transcriptome profiling here were validated using real time quantitative PCR in ten selected genes. The present study provided insights into the transcriptome profile of ovarian tissues from diploid and tetraploid loaches Misgurnus anguillicaudatus, which was made available to the research community for functional genomics, comparative genomics, polyploidy evolution and molecular breeding of this loach and other related species.
Synthesis and Characterisation of the Europium (III) Dimolybdo-Enneatungsto-Silicate Dimer, [Eu(α-SiW9Mo2O39)2]13−
Authors: Parent Loïc , De Oliveira Pedro , Teillout Anne-Lucie , Dolbecq Anne , Haouas Mohamed , Cadot Emmanuel , Mbomekallé Israël
Published: July 2015

The chemistry of polyoxometalates (POMs) keeps drawing the attention of researchers, since they constitute a family of discrete molecular entities whose features may be easily modulated. Often considered soluble molecular oxide analogues, POMs possess enormous potential due to a myriad of choices concerning size, shape and chemical composition that may be tailored in order to fine-tune their physico-chemical properties. Thanks to the recent progress in single-crystal X ray diffraction, new POMs exhibiting diverse and unexpected structures have been regularly reported and described. We find it relevant to systematically analyse the different equilibria that govern the formation of POMs, in order to be able to establish reliable synthesis protocols leading to new molecules. In this context, we have been able to synthesise the Eu3+-containing silico-molybdo-tungstic dimer, [Eu(α-SiW9Mo2O39)2]13−. We describe the synthesis and characterisation of this new species by several physico-chemical methods, such as single-crystal X-ray diffraction, 183W NMR and electrochemistry.
Investigation of the Matrix Metalloproteinase-2 Gene in Patients with Non-Syndromic Mitral Valve Prolapse
Authors: Perrocheau Maëlle , Kiando Soto , Vernerey Déwi , Dina Christian , Galan Pilar , Hagege Albert , Jeunemaitre Xavier , Bouatia-Naji Nabila
Published: July 2015

Non-syndromic mitral valve prolapse (MVP) is a common degenerative valvulopathy, predisposing to arrhythmia and sudden death. The etiology of MVP is suspected to be under genetic control, as supported by familial cases and its manifestation in genetic syndrome (e.g., Marfan syndrome). One candidate etiological mechanism is a perturbation of the extracellular matrix (ECM) remodeling of the valve. To test this hypothesis, we assessed the role of genetic variants in the matrix metalloproteinase 2 gene (MMP2) known to regulate the ECM turnover by direct degradation of proteins and for which transgenic mice develop MVP. Direct sequencing of exons of MMP2 in 47 unrelated patients and segregation analyses in families did not reveal any causative mutation. We studied eight common single nucleotide polymorphisms (TagSNPs), which summarize the genetic information at the MMP2 locus. The association study in two case controls sets (NCases = 1073 and NControls = 1635) provided suggestive evidence for the association of rs1556888 located downstream MMP2 with the risk of MVP, especially in patients with the fibroelastic defiency form. Our study does not support the contribution of MMP2 rare variation in the etiology to MVP in humans, though further genetic and molecular investigation is required to confirm our current suggestive association of one common variant.
Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications
Authors: Hossain Kazi , Zhu Chenkai , Felfel Reda , Sharmin Nusrat , Ahmed Ifty
Published: July 2015

Tubular scaffolds with aligned polylactic acid (PLA) fibres were fabricated for cell guide applications by immersing rolled PLA fibre mats into a polyvinyl acetate (PVAc) solution to bind the mats. The PVAc solution was also mixed with up to 30 wt % β-tricalcium phosphate (β-TCP) content. Cross-sectional images of the scaffold materials obtained via scanning electron microscopy (SEM) revealed the aligned fibre morphology along with a significant number of voids in between the bundles of fibres. The addition of β-TCP into the scaffolds played an important role in increasing the void content from 17.1% to 25.3% for the 30 wt % β-TCP loading, which was measured via micro-CT (µCT) analysis. Furthermore, µCT analyses revealed the distribution of aggregated β-TCP particles in between the various PLA fibre layers of the scaffold. The compressive modulus properties of the scaffolds increased from 66 MPa to 83 MPa and the compressive strength properties decreased from 67 MPa to 41 MPa for the 30 wt % β-TCP content scaffold. The scaffolds produced were observed to change into a soft and flexible form which demonstrated shape recovery properties after immersion in phosphate buffered saline (PBS) media at 37 °C for 24 h. The cytocompatibility studies (using MG-63 human osteosarcoma cell line) revealed preferential cell proliferation along the longitudinal direction of the fibres as compared to the control tissue culture plastic. The manufacturing process highlighted above reveals a simple process for inducing controlled cell alignment and varying porosity features within tubular scaffolds for potential tissue engineering applications.
How to Make a Barranco: Modeling Erosion and Land-Use in Mediterranean Landscapes
Authors: Barton C. , Ullah Isaac , Heimsath Arjun
Published: July 2015

We use the hybrid modeling laboratory of the Mediterranean Landscape Dynamics (MedLanD) Project to simulate barranco incision in eastern Spain under different scenarios of natural and human environmental change. We carry out a series of modeling experiments set in the Rio Penaguila valley of northern Alicante Province. The MedLanD Modeling Laboratory (MML) is able to realistically simulate gullying and incision in a multi-dimensional, spatially explicit virtual landscape. We first compare erosion modeled in wooded and denuded landscapes in the absence of human land-use. We then introduce simulated small-holder (e.g., prehistoric Neolithic) farmer/herders in six experiments, by varying community size (small, medium, large) and land management strategy (satisficing and maximizing). We compare the amount and location of erosion under natural and anthropogenic conditions. Natural (e.g., climatically induced) land-cover change produces a distinctly different signature of landscape evolution than does land-cover change produced by agropastoral land-use. Human land-use induces increased coupling between hillslopes and channels, resulting in increased downstream incision.
3,4-Dihydro-3-(2-hydroxyethyl)-4-(nitromethyl)quinazolin-2(1H)-one
Authors: Sawant Rajiv , Stevens Marc , Odell Luke
Published: July 2015

A one-pot, direct synthesis of 3,4-dihydro-3-(2-hydroxyethyl)-4-(nitromethyl)quinazolin-2(1H)-one from methyl (2-formylphenyl)carbamate, ethanolamine and nitromethane in AcOH is reported. The reaction proceeds via a cascade three-component aza-Henry reaction under microwave irradiation and the title compound was characterized by 1H NMR, 13C NMR and ESI/MS analysis.
Photophysical Study of Polymer-Based Solar Cells with an Organo-Boron Molecule in the Active Layer
Authors: Romero-Servin Sergio , Villa Manuel , Carriles R. , Ramos-Ortíz Gabriel , Maldonado José-Luis , Rodríguez Mario , Güizado-Rodríguez M.
Published: July 2015

Our group previously reported the synthesis of four polythiophene derivatives (P1–P4) used for solar cells. The cells were prepared under room conditions by spin coating, leading to low efficiencies. However, after the addition of 6-nitro-3-(E)-3-(4-dimethylaminophenyl)allylidene)-2,3-dihydrobenzo[d]-[1,3,2] oxazaborole (M1) to their active layers, the efficiencies of the cells showed approximately a two-fold improvement. In this paper, we study this enhancement mechanism by performing ultrafast transient absorption (TA) experiments on the active layer of the different cells. Our samples consisted of thin films of a mixture of PC61BM with the polythiophenes derivatives P1–P4. We prepared two versions of each sample, one including the molecule M1 and another without it. The TA data suggests that the efficiency improvement after addition of M1 is due not only to an extended absorption spectrum towards the infrared region causing a larger population of excitons but also to the possible creation of additional channels for transport of excitons and/or electrons to the PC61BM interface.
Preparation and Characterization of Surface Photocatalytic Activity with NiO/TiO2 Nanocomposite Structure
Authors: Chen Jian-Zhi , Chen Tai-Hong , Lai Li-Wen , Li Pei-Yu , Liu Hua-Wen , Hong Yi-You , Liu Day-Shan
Published: July 2015

This study achieved a nanocomposite structure of nickel oxide (NiO)/titanium dioxide (TiO2) heterojunction on a TiO2 film surface. The photocatalytic activity of this structure evaluated by decomposing methylene blue (MB) solution was strongly correlated to the conductive behavior of the NiO film. A p-type NiO film of high concentration in contact with the native n-type TiO2 film, which resulted in a strong inner electrical field to effectively separate the photogenerated electron-hole pairs, exhibited a much better photocatalytic activity than the controlled TiO2 film. In addition, the photocatalytic activity of the NiO/TiO2 nanocomposite structure was enhanced as the thickness of the p-NiO film decreased, which was beneficial for the migration of the photogenerated carriers to the structural surface.
Identification of the Scopularide Biosynthetic Gene Cluster in Scopulariopsis brevicaulis
Authors: Lukassen Mie , Saei Wagma , Sondergaard Teis , Tamminen Anu , Kumar Abhishek , Kempken Frank , Wiebe Marilyn , Sørensen Jens
Published: July 2015

Scopularide A is a promising potent anticancer lipopeptide isolated from a marine derived Scopulariopsis brevicaulis strain. The compound consists of a reduced carbon chain (3-hydroxy-methyldecanoyl) attached to five amino acids (glycine, l-valine, d-leucine, l-alanine, and l-phenylalanine). Using the newly sequenced S. brevicaulis genome we were able to identify the putative biosynthetic gene cluster using genetic information from the structurally related emericellamide A from Aspergillus nidulans and W493-B from Fusarium pseudograminearum. The scopularide A gene cluster includes a nonribosomal peptide synthetase (NRPS1), a polyketide synthase (PKS2), a CoA ligase, an acyltransferase, and a transcription factor. Homologous recombination was low in S. brevicaulis so the local transcription factor was integrated randomly under a constitutive promoter, which led to a three to four-fold increase in scopularide A production. This indirectly verifies the identity of the proposed biosynthetic gene cluster.
A Concentration-Controllable Microfluidic Droplet Mixer for Mercury Ion Detection
Authors: Meng Qian-Fang , Rao Lang , Cai Bo , You Su-Jian , Guo Shi-Shang , Liu Wei , Zhao Xing-Zhong
Published: July 2015

A microfluidic droplet mixer is developed for rapid detection of Hg(II) ions. Reagent concentration and droplets can be precisely controlled by adjusting the flow rates of different fluid phases. By selecting suitable flow rates of the oil phase, probe phase and sample phase, probe droplets and sample droplets can be matched and merged in pairs and subsequently well-mixed in the poly (dimethylsiloxane) (PDMS) channels. The fluorescence enhancement probe (Rhodamine B mixed with gold nanoparticles) encapsulated in droplets can react with Hg(II) ions. The Hg(II) ion concentration in the sample droplets is adjusted from about 0 to 1000 nM through fluid regulation to simulate possible various contaminative water samples. The intensity of the emission fluorescence is sensitive to Hg(II) ions (increases as the Hg(II) ion concentration increases). Through the analysis of the acquired fluorescence images, the concentration of Hg(II) ions can be precisely detected. With the advantages of less time, cost consumption and easier manipulations, this device would have a great potential in micro-scale sample assays and real-time chemical reaction studies.
CAPE Analogs Induce Growth Arrest and Apoptosis in Breast Cancer Cells
Authors: Beauregard Annie-Pier , Harquail Jason , Lassalle-Claux Grégoire , Belbraouet Mehdi , Jean-Francois Jacques , Touaibia Mohamed , Robichaud Gilles
Published: July 2015

Breast cancer is the second leading cause of death amongst women worldwide. As a result, many have turned their attention to new alternative approaches to treat this disease. Caffeic acid phenylethyl ester (CAPE), a well-known active compound from bee propolis, has been previously identified as a strong antioxidant, anti-inflammatory, antiviral and anticancer molecule. In fact, CAPE is well documented as inducing cell death by inhibiting NFκB and by inducing pro-apoptotic pathways (i.e., p53). With the objective of developing stronger anticancer compounds, we studied 18 recently described CAPE derivatives for their ability to induce apoptosis in breast cancer cell lines. Five of the said compounds, including CAPE, were selected and subsequently characterised for their anticancer mechanism of action. We validated that CAPE is a potent inducer of caspase-dependent apoptosis. Interestingly, some newly synthesized CAPE derivatives also showed greater cell death activity than the lead CAPE structure. Similarly to CAPE, analog compounds elicited p53 activation. Interestingly, one compound in particular, analog 10, induced apoptosis in a p53-mutated cell line. These results suggest that our new CAPE analog compounds may display the capacity to induce breast cancer apoptosis in a p53-dependent and/or independent manner. These CAPE analogs could thus provide new therapeutic approaches for patients with varying genotypic signatures (such as p53 mutations) in a more specific and targeted fashion.
Development of Guanidine-Bisurea Bifunctional Organocatalysts with a Chiral Pyrrolidine Moiety and Application to α-Hydroxylation of Tetralone-Derived β-Keto Esters
Authors: Odagi Minami , Takayama Kan , Sato Makoto , Yamanaka Masahiro , Nagasawa Kazuo
Published: July 2015

Novel guanidine-bisurea bifunctional organocatalysts 5 bearing a chiral pyrrolidine moiety on guanidine were designed with the guidance of DFT calculations. The resulting organocatalysts 5 were examined for α-hydroxylation of tetralone-derived β-keto esters, and good selectivity was obtained with 5j bearing a methoxymethyl ether-containing chiral pyrrolidine moiety.
Simultaneous Fitting of Absorption Spectra and Their Second Derivatives for an Improved Analysis of Protein Infrared Spectra
Authors: Baldassarre Maurizio , Li Chenge , Eremina Nadejda , Goormaghtigh Erik , Barth Andreas
Published: July 2015

Infrared spectroscopy is a powerful tool in protein science due to its sensitivity to changes in secondary structure or conformation. In order to take advantage of the full power of infrared spectroscopy in structural studies of proteins, complex band contours, such as the amide I band, have to be decomposed into their main component bands, a process referred to as curve fitting. In this paper, we report on an improved curve fitting approach in which absorption spectra and second derivative spectra are fitted simultaneously. Our approach, which we name co-fitting, leads to a more reliable modelling of the experimental data because it uses more spectral information than the standard approach of fitting only the absorption spectrum. It also avoids that the fitting routine becomes trapped in local minima. We have tested the proposed approach using infrared absorption spectra of three mixed α/β proteins with different degrees of spectral overlap in the amide I region: ribonuclease A, pyruvate kinase, and aconitase.
Non-Nucleosidic Analogues of Polyaminonucleosides and Their Influence on Thermodynamic Properties of Derived Oligonucleotides
Authors: Brzezinska Jolanta , Markiewicz Wojciech
Published: July 2015

The rationale for the synthesis of cationic modified nucleosides is higher expected nuclease resistance and potentially better cellular uptake due to an overall reduced negative charge based on internal charge compensation. Due to the ideal distance between cationic groups, polyamines are perfect counterions for oligodeoxyribonucleotides. We have synthesized non-nucleosidic analogues built from units that carry different diol structures instead of sugar residues and functionalized with polyamines. The non-nucleosidic analogues were attached as internal or 5′-terminal modifications in oligodeoxyribonucleotide strands. The thermodynamic studies of these polyaminooligonucleotide analogues revealed stabilizing or destabilizing effects that depend on the linker or polyamine used.
Synthesis, Structure and Antioxidant Activity of Cyclohexene-Fused Selenuranes and Related Derivatives
Authors: Prasad Poonam , Singh Harkesh , Butcher Ray
Published: July 2015

Synthesis, structure and antioxidant activity of new cyclohexene-fused spiroselenuranes and a spirotellurane is reported. Oxidation reactions of bis(o-formylcyclohex- 1-ene)selenide/bis(2-hydroxymethylcyclohex-1-ene)selenide provide the corresponding spiroselenuranes. The glutathione peroxidase-like activity of the newly synthesized compounds has been evaluated.
Glucose Homeostasis Variables in Pregnancy versus Maternal and Infant Body Composition
Authors: Henriksson Pontus , Löf Marie , Forsum Elisabet
Published: July 2015

Intrauterine factors influence infant size and body composition but the mechanisms involved are to a large extent unknown. We studied relationships between the body composition of pregnant women and variables related to their glucose homeostasis, i.e., glucose, HOMA-IR (homeostasis model assessment-insulin resistance), hemoglobin A1c and IGFBP-1 (insulin-like growth factor binding protein-1), and related these variables to the body composition of their infants. Body composition of 209 women in gestational week 32 and of their healthy, singleton and full-term one-week-old infants was measured using air displacement plethysmography. Glucose homeostasis variables were assessed in gestational week 32. HOMA-IR was positively related to fat mass index and fat mass (r2 = 0.32, p < 0.001) of the women. Maternal glucose and HOMA-IR values were positively (p ≤ 0.006) associated, while IGFBP-1was negatively (p = 0.001) associated, with infant fat mass. HOMA-IR was positively associated with fat mass of daughters (p < 0.001), but not of sons (p = 0.65) (Sex-interaction: p = 0.042). In conclusion, glucose homeostasis variables of pregnant women are related to their own body composition and to that of their infants. The results suggest that a previously identified relationship between fat mass of mothers and daughters is mediated by maternal insulin resistance.
Optimal Dietary and Plasma Magnesium Statuses Depend on Dietary Quality for a Reduction in the Risk of All-Cause Mortality in Older Adults
Authors: Huang Yi-Chen , Wahlqvist Mark , Kao Mei-Ding , Wang Jui-Lien , Lee Meei-Shyuan
Published: July 2015

The association between dietary or plasma magnesium (Mg) with diabetes incidence and with mortality in free-living elderly was investigated. A total of 1400 participants from the Taiwanese Nutrition Survey, aged ≥ 65 years, and diabetes-free from the 1999–2000 were assessed. The dietary intake and plasma Mg concentration were obtained through 24h dietary recall and health examination at baseline. Participants were classified by quartiles (Q) of dietary Mg or by the plasma Mg normal range (0.75–0.95 mmol/L). Dietary diversity score (DDS, range 1–6) represented the dietary quality. During 8 and 10 years, 231 incident diabetes cases and 475 deaths were identified. Cox’s proportional-hazards regression was used to evaluate the association between Mg and health outcomes. The hazard ratios (95% confidence interval) for death in Q2 and Q3 of Mg intakes with DDS > 4 were 0.57 (0.44–0.74) and 0.59 (0.39–0.88), respectively, compared with the lowest intake and DDS ≤ 4 participants. Participants with normal and high plasma Mg in conjunction with high DDS had relative risks of 0.58 (0.37–0.89) and 0.46 (0.25–0.85) in mortality compared with low plasma Mg and lower DDS. Optimal dietary Mg intake and plasma Mg depend on dietary quality to reduce the mortality risk in older adults.
Peptides and Peptidomimetics for Antimicrobial Drug Design
Authors: Mojsoska Biljana , Jenssen Håvard
Published: July 2015

The purpose of this paper is to introduce and highlight a few classes of traditional antimicrobial peptides with a focus on structure-activity relationship studies. After first dissecting the important physiochemical properties that influence the antimicrobial and toxic properties of antimicrobial peptides, the contributions of individual amino acids with respect to the peptides antibacterial properties are presented. A brief discussion of the mechanisms of action of different antimicrobials as well as the development of bacterial resistance towards antimicrobial peptides follows. Finally, current efforts on novel design strategies and peptidomimetics are introduced to illustrate the importance of antimicrobial peptide research in the development of future antibiotics.
pH-Dependent Release of Insulin from Layer-by-Layer-Deposited Polyelectrolyte Microcapsules
Authors: Yoshida Kentaro , Ono Tetsuya , Kashiwagi Yoshitomo , Takahashi Shigehiro , Sato Katsuhiko , Anzai Jun-ichi
Published: July 2015

Insulin-containing microcapsules were prepared by a layer-by-layer (LbL) deposition of poly(allylamine hydrochloride) (PAH) and polyanions, such as poly(styrenesulfonate) (PSS), poly(vinyl sulfate) (PVS), and dextran sulfate (DS) on insulin-containing calcium carbonate (CaCO3) microparticles. The CaCO3 core was dissolved in diluted HCl solution to obtain insulin-containing hollow microcapsules. The microcapsules were characterized by scanning electron microscope (SEM) and atomic force microscope (AFM) images and ζ-potential. The release of insulin from the microcapsules was faster at pH 9.0 and 7.4 than in acidic solutions due to the different charge density of PAH. In addition, insulin release was suppressed when the microcapsules were constructed using PAH with a lower molecular weight, probably owing to a thicker shell of the microcapsules. The results suggested a potential use of the insulin-containing microcapsules for developing insulin delivery systems.
Life Cycle Network Modeling Framework and Solution Algorithms for Systems Analysis and Optimization of the Water-Energy Nexus
Authors: Garcia Daniel , You Fengqi
Published: July 2015

The water footprint of energy systems must be considered, as future water scarcity has been identified as a major concern. This work presents a general life cycle network modeling and optimization framework for energy-based products and processes using a functional unit of liters of water consumed in the processing pathway. We analyze and optimize the water-energy nexus over the objectives of water footprint minimization, maximization of economic output per liter of water consumed (economic efficiency of water), and maximization of energy output per liter of water consumed (energy efficiency of water). A mixed integer, multiobjective nonlinear fractional programming (MINLFP) model is formulated. A mixed integer linear programing (MILP)-based branch and refine algorithm that incorporates both the parametric algorithm and nonlinear programming (NLP) subproblems is developed to boost solving efficiency. A case study in bioenergy is presented, and the water footprint is considered from biomass cultivation to biofuel production, providing a novel perspective into the consumption of water throughout the value chain. The case study, optimized successively over the three aforementioned objectives, utilizes a variety of candidate biomass feedstocks to meet primary fuel products demand (ethanol, diesel, and gasoline). A minimum water footprint of 55.1 ML/year was found, economic efficiencies of water range from −$1.31/L to $0.76/L, and energy efficiencies of water ranged from 15.32 MJ/L to 27.98 MJ/L. These results show optimization provides avenues for process improvement, as reported values for the energy efficiency of bioethanol range from 0.62 MJ/L to 3.18 MJ/L. Furthermore, the proposed solution approach was shown to be an order of magnitude more efficient than directly solving the original MINLFP problem with general purpose solvers.
Reason, Rhythm, and Rituality. Reinterpreting Religious Cult from a Postmodern, Phenomenological Perspective
Authors: Roesner Martina
Published: July 2015

Contemporary philosophy of religion is often focused, at a theoretical level, on the epistemic value of religious doctrines, and at a practical level, on the possible impact of organized religion on secular society and politics. However, the cultic dimension of religion, such as prayer, religious service, ascetic practices, and other rituals, is considered as completely “irrational” and incomprehensible from a secular perspective and therefore often neglected by postmodern philosophy. The paper intends to call into question this rather simplistic interpretation by retracing the historical origins of the devaluation of religious symbolism in occidental thought, which culminates in Kant’s philosophy of religion. We then shall analyze to what extent certain paradoxical aspects of Habermas’ view on religion can be interpreted as consequences of the dilemma brought about by the Kantian dichotomy between man as moral subject and man as natural, sensible being. In a third step, we shall develop an alternative, phenomenological interpretation, which does not consider religious practice as a primitive, irrational phenomenon but as a proto-ethical schematism that aims at integrating the sphere of pure practical reason into the rhythmic structure of living, embodied consciousness.