bluuebunny/crossref_metadata_2025 huggingface dataset:
The Importance of Nitric Oxide as the Molecular Basis of the Hydrogen Gas Fumigation-Induced Alleviation of Cd Stress on Ganoderma lucidum
Authors: Abdalmegeed Dyaaaldin , Zhao Gan , Cheng Pengfei , Bhat Javaid A. , Khattak Wajid Ali , Ali Mostafa G. , Alnadari Fawze , Ali Ilyas , Ali Qurban , Korma Sameh A. , Mahmoud Yehia A.-G. , Abd Elnabi Manar K. , Cui Weiti , Shen Wenbiao
Published: December 2021

Whether or not hydrogen gas (H2) can reduce cadmium (Cd) toxicity in Ganoderma lucidum has remained largely unknown. Here, we report that Cd-induced growth inhibition in G. lucidum was significantly alleviated by H2 fumigation or hydrogen-rich water (HRW), evaluated by lower oxidative damage and Cd accumulation. Moreover, the amelioration effects of H2 fumigation were better than of HRW in an optimum concentration of H2 under our experimental conditions. Further results showed that H2-alleviated growth inhibition in G. lucidum was accompanied by increased nitric oxide (NO) level and nitrate reductase (NR) activity under Cd stress. On the other hand, the mitigation effects were reversed after removing endogenous NO with its scavenger cPTIO or inhibiting H2-induced NR activity with sodium tungstate. The role of NO in H2-alleviated growth inhibition under Cd stress was proved to be achieved through a restoration of redox balance, an increase in cysteine and proline contents, and a reduction in Cd accumulation. In summary, these results clearly revealed that NR-dependent NO might be involved in the H2-alleviated Cd toxicity in G. lucidum through rebuilding redox homeostasis, increasing cysteine and proline levels, and reducing Cd accumulation. These findings may open a new window for H2 application in Cd-stressed economically important fungi.
Verification Systems and Programs in Regional Television Stations That Are Members of the CIRCOM Network
Authors: Rúas-Araújo José , Rodríguez-Martelo Talía , Máiz-Bar Carmen
Published: December 2021

Disinformation and the proliferation of fake news are global problems that affect the stability of democracies throughout the world. The capacity of distorted information to interfere in election processes or in political agendas has led different actors to create verification initiatives, which operate in partnership with the mass media. Recently, during the 2020 health crisis, false information has proved to have damaging power not only at the levels of politics or communication, but also at a health level. Therefore, the social need to access reliable and quality information, as well as verified information aimed at eradicating hoaxes, becomes evident. This paper focuses on the European context, analyzing the relationship between active verifiers and television stations that are members of the CIRCOM Network, considering their strategies and verification programs. Using a qualitative methodology an exploratory study has been carried out, mapping initiatives and stations by assessing their contribution of verified information to society.
Forest Transition and Metropolitan Transformations in Developed Countries: Interpreting Apparent and Latent Dynamics with Local Regression Models
Authors: Bianchini Leonardo , Salvia Rosanna , Quaranta Giovanni , Egidi Gianluca , Salvati Luca , Marucci Alvaro
Published: December 2021

Metropolitan fringes in Southern Europe preserve, under different territorial contexts, natural habitats, relict woodlands, and mixed agro-forest systems acting as a sink of biodiversity and ecosystem services in ecologically vulnerable landscapes. Clarifying territorial and socioeconomic processes that underlie land-use change in metropolitan regions is relevant for forest conservation policies. At the same time, long-term dynamics of fringe forests in the northern Mediterranean basin have been demonstrated to be rather mixed, with deforestation up to the 1950s and a subsequent recovery more evident in recent decades. The present study makes use of Forest Transition Theory (FTT) to examine spatial processes of forest loss and expansion in metropolitan Rome, Central Italy, through local regressions elaborating two diachronic land-use maps that span more than 80 years (1936–2018) representative of different socioeconomic and ecological conditions. Our study evaluates the turnaround from net forest area loss to net forest area gain, considering together the predictions of the FTT and those of the City Life Cycle (CLC) theory that provides a classical description of the functioning of metropolitan cycles. The empirical findings of our study document a moderate increase in forest cover depending on the forestation of previously abandoned cropland as a consequence of tighter levels of land protection. Natural and human-driven expansion of small and isolated forest nuclei along fringe land was demonstrated to fuel a polycentric expansion of woodlands. The results of a Geographically Weighted Regression (GWR) reveal the importance of metropolitan growth in long-term forest expansion. Forest–urban dynamics reflect together settlement sprawl and increased forest disturbance. The contemporary expansion of fringe residential settlements and peri-urban forests into relict agricultural landscapes claims for a renewed land management that may reconnect town planning, reducing the intrinsic risks associated with fringe woodlands (e.g., wildfires) with environmental policies preserving the ecological functionality of diversified agro-forest systems.
Does Land Transfer Improve Farmers’ Quality of Life? Evidence from Rural China
Authors: He Qiang , Deng Xin , Li Chuan , Kong Fangxia , Qi Yanbin
Published: December 2021

The topic of quality of life has long been a focus of global research and the public. The land transfer policy implemented by the Chinese government affects farmers’ quality of life (FQOL); however, the extent of this effect remains unclear. As land transfer may be a self-selection behaviour, it may be subject to selection bias such that traditional measurement methods are unable to effectively estimate its quantitative impact. This study used data from a questionnaire given to 5668 rural households in 25 provinces of mainland China. It sought to quantify the impact of land transfer on FQOL by using endogenous switching regression (ESR) models to correct selection bias. The results show: (1) for farmers who choose to transfer land, if they choose not to transfer land, FQOL may decrease by 64.11%; (2) for farmers who choose not to transfer their land, if they go on to choose to transfer their land, FQOL may increase by 0.75%; (3) land transfer can improve the quality of life of the older generation of farmers but will reduce the quality of life of the newer generation. The results of this study provide research support for China and other countries seeking to effectively implement land policies and improve the FQOL, helping to provide practical strategies for the sustainable development of rural areas.
The Challenge of Social Vulnerability Assessment in the Context of Land Use Changes for Sustainable Urban Planning—Case Studies: Developing Cities in Romania
Authors: Botezan Camelia Sabina , Radovici Andrei , Ajtai Iulia
Published: December 2021

Urban growth triggers massive changes in land use cover, exacerbating extreme natural and technological events. In order for land use planning to be efficient, it requires the integration of comprehensive risk and vulnerability assessment. This paper aims to create a bridge between the existing vulnerability theories and their implementation in land use planning policies and proposes an innovative approach to determine whether the changes in the territorial dynamics of cities draw considerable changes in communities’ social vulnerability. The methodology identifies and selects three case studies from the Urban Atlas inventory, representative of the dynamics of large Romanian cities, taking into consideration the following hazards: earthquakes, floods, and technological hazards. Vulnerability was then assessed by assigning each land use class a specific vulnerability level. The methodology involved assessing the level of vulnerability specific to the situation in 2018 compared to 2006. The results showed that major changes in land use are related to the transition of areas with a low level of vulnerability to areas with a higher level of vulnerability as a result of the urban areas expansion to the detriment of natural and agricultural areas. This is generally translated into a higher degree of vulnerability due to an increased density of artificial elements and of population in the residential areas. The findings of the study of territorial dynamics in the proximity of large industrial operators did not reveal a tendency that differed from the general trend. Although many territorial changes have been observed in the period 2006–2018, it is necessary to extend the analysis, with the issue of the new versions of the Urban Atlas, to confirm the identified trends and to express the up-to-date situation.
Preliminary Experimental Trial of Effects of Lattice Fence Installation on Honey Bee Flight Height as Implications for Urban Beekeeping Regulations
Authors: Matsuzawa Tomonori , Kohsaka Ryo
Published: December 2021

Urban beekeeping has gained salience because of its significance in biodiversity conservation and community building. Despite this, beekeeping practices in urban areas have received negative perceptions from residents, which stem from public safety concerns. There is, therefore, a need to enhance and/or work on appropriate rules for maximizing the profits while minimizing the risks. Amongst the present regulations, the installation of barriers and setbacks is the most common rule for public safety. However, only a limited number of empirical studies have reported on their effective location and height. Thus, in this study, an experimental apiary was set up with different types of barriers installed with varying distances to observe and measure flyway patterns of honey bees. We used a 3D laser scanner, which obtained 8529 points of highly accurate flight location data in about five hours. Results showed that the heights (1.8 and 0.9 m) of the barriers installed were effective in increasing the flight altitudes. The distance of the fence, which was installed as close as 1 m from the hives, was effective as well. These findings, which showed that barriers and setbacks are effective, can have regulatory implications in designing apiaries in urban spaces, where location is often restricted.
Major Surgical Trauma Impairs the Function of Natural Killer Cells but Does Not Affect Monocyte Cytokine Synthesis
Authors: Müller-Heck Roman M. , Bösken Björn , Michiels Ivo , Dudda Marcel , Jäger Marcus , Flohé Stefanie B.
Published: December 2021

Major traumatic and surgical injury increase the risk for infectious complications due to immune dysregulation. Upon stimulation with interleukin (IL) 12 by monocyte/macrophages, natural killer (NK) cells release interferon (IFN) γ that supports the elimination of the pathogen. In the present study, we investigated the impact of invasive spine surgery on the relationship between monocytes and NK cells upon exposure to Staphylococcus aureus. Mononuclear cells and serum were isolated from peripheral blood of patients before and up to 8 d after surgery and stimulated with inactivated S. aureus bacteria. NK cell and monocyte function were determined by flow cytometry. NK cells continuously lost their ability to produce IFN-γ during the first week after surgery independently from monocyte-derived IL-12 secretion. IFN-γ synthesis was minimal on day 8 and was associated with decreased expression of the IL-12 receptor and activation of transcription factors required for IFNG gene transcription. Addition of recombinant IL-12 could at least partially restore NK cell function. Pre-operative levels of growth/differentiation factor (GDF) 15 in the serum correlated with the extent of NK cell suppression and with hospitalization. Thus, NK cell suppression after major surgery might represent a therapeutic target to improve the immune defense against opportunistic infections.
The Basolateral Amygdala Mediates the Role of Rapid Eye Movement Sleep in Integrating Fear Memory Responses
Authors: Machida Mayumi , Sweeten Brook , Adkins Austin , Wellman Laurie , Sanford Larry
Published: December 2021

The basolateral amygdala (BLA) mediates the effects of stress and fear on rapid eye movement sleep (REM) and on REM-related theta (θ) oscillatory activity in the electroencephalograph (EEG), which is implicated in fear memory consolidation. We used optogenetics to assess the potential role of BLA glutamate neurons (BLAGlu) in regulating behavioral, stress and sleep indices of fear memory, and their relationship to altered θ. An excitatory optogenetic construct targeting glutamatergic cells (AAV-CaMKIIα-hChR2-eYFP) was injected into the BLA of mice. Telemetry was used for real-time monitoring of EEG, activity, and body temperature to determine sleep states and stress-induced hyperthermia (SIH). For 3 h following shock training (ST: 20 footshocks, 0.5 mA, 0.5 s, 1 min interval), BLA was optogenetically stimulated only during REM (REM + L) or NREM (NREM + L). Mice were then re-exposed to the fear context at 24 h, 48 h, and 1 week after ST and assessed for behavior, SIH, sleep and θ activity. Control mice were infected with a construct without ChR2 (eYFP) and studied under the same conditions. REM + L significantly reduced freezing and facilitated immediate recovery of REM tested at 24 h and 48 h post-ST during contextual re-exposures, whereas NREM + L had no significant effect. REM + L significantly reduced post-ST REM-θ, but attenuated REM-θ reductions at 24 h compared to those found in NREM + L and control mice. Fear-conditioned SIH persisted regardless of treatment. The results demonstrate that BLAGlu activity during post-ST REM mediates the integration of behavioral and sleep indices of fear memory by processes that are associated with θ oscillations within the amygdalo-hippocampal pathway. They also demonstrate that fear memories can remain stressful (as indicated by SIH) even when fear conditioned behavior (freezing) and changes in sleep are attenuated.
Microstructure Investigation of WC-Based Coatings Prepared by HVOF onto AZ31 Substrate
Authors: Jonda Ewa , Łatka Leszek , Tomiczek Anna , Godzierz Marcin , Pakieła Wojciech , Nuckowski Paweł
Published: December 2021

In this paper, three commercial cermet powders, WC-Co-Cr, WC-Co and WC-Cr3C2-Ni, were sprayed by the High Velocity Oxy Fuel (HVOF) method onto magnesium alloy AZ31 substrate. The coatings were investigated in terms of their microstructure, phase analysis and residual stress. The manufactured coatings were analyzed extensively using optical microscopy (OM), X-ray diffraction (XRD), scanning (SEM) and transmission electron microscopy (TEM). Based on microstructure studies, it was noted that the coatings show satisfactory homogeneity. XRD analysis shows that in WC-Co, WC-Co-Cr and WC-Cr3C2-Ni coatings, main peaks are related to WC. Weaker peaks such as W2C, Co0.9W0.1, Co and W for WC-Co and W2C, Cr3C2 and Cr7C3 for WC-Cr3C2-Ni also occur. In all cermet coatings, linear stress showed compressive nature. In WC-Co and WC-Cr3C2-Ni, residual stress had a similar value, while in WC-Co-Cr, linear stress was lower. It was also proved that spraying onto magnesium substrate causes shear stress in the WC phase, most likely due to the low elastic modulus of magnesium alloy substrate.
Half Metallic Ferromagnetism and Transport Properties of Zinc Chalcogenides ZnX2Se4 (X = Ti, V, Cr) for Spintronic Applications
Authors: Al-Qhtani Mohsen , Mustafa Ghulam , Mazhar Nasheeta , Bouzgarrou Sonia , Mahmood Qasim , Mera Abeer , Zaki Zaki , Mostafa Nasser , Alotaibi Saad , Amin Mohammed
Published: December 2021

In ferromagnetic semiconductors, the coupling of magnetic ordering with semiconductor character accelerates the quantum computing. The structural stability, Curie temperature (Tc), spin polarization, half magnetic ferromagnetism and transport properties of ZnX2Se4 (X = Ti, V, Cr) chalcogenides for spintronic and thermoelectric applications are studied here by density functional theory (DFT). The highest value of Tc is perceived for ZnCr2Se4. The band structures in both spin channels confirmed half metallic ferromagnetic behavior, which is approved by integer magnetic moments (2, 3, 4) μB of Ti, V and Cr based spinels. The HM behavior is further measured by computing crystal field energy ΔEcrystal, exchange energies Δx(d), Δx (pd) and exchange constants (Noα and Noβ). The thermoelectric properties are addressed in terms of electrical conductivity, thermal conductivity, Seebeck coefficient and power factor in within a temperature range 0–400 K. The positive Seebeck coefficient shows p-type character and the PF is highest for ZnTi2Se4 (1.2 × 1011 W/mK2) among studied compounds.
The Influence of the Soaking Temperature Rotary Forging and Solution Heat Treatment on the Structural and Mechanical Behavior in Ni-Rich NiTi Alloy
Authors: Freitas Rodrigues Patrícia , Teixeira Rodolfo S. , Le Sénéchal Naiara V. , Braz Fernandes Francisco Manuel , Paula Andersan S.
Published: December 2021

The structural and thermophysical characteristics of an Ni-rich NiTi alloy rod produced on a laboratory scale was studied. The soak temperature of the solution heat-treatment steps above 850 °C taking advantage of the precipitate dissolution to provide a matrix homogenization, but it takes many hours (24 to 48) when used without thermomechanical steps. Therefore, the suitable reheating to apply between the forging process steps is very important, because the product’s structural characteristics are dependent on the thermomechanical processing history, and the time required to expose the material to high temperatures during the processing is reduced. The structural characteristics were investigated after solution heat treatment at 900 °C and 950 °C for 120 min, and these heat treatments were compared with as-forged sample structural characteristics (one hot deformation step after 800 °C for a 30 min reheat stage). The phase-transformation temperatures were analyzed through differential scanning calorimetry (DSC), and the structural characterization was performed through synchrotron radiation-based X-ray diffraction (SR-XRD) at room temperature. It was observed that the solution heat treatment at 950 °C/120 min presents a lower martensitic reversion finish temperature (Af); the matrix was fully austenitic; and it had a hardness of about 226 HV. Thus, this condition is the most suitable for the reheating stages between the hot forging-process steps to be applied to this alloy to produce materials that can display a superelasticity effect, for applications such as crack sensors or orthodontic archwires.
Clay Mineral Minerals as a Strategy for Biomolecule Incorporation: Amino Acids Approach
Authors: Brandão-Lima Luciano C. , Silva Fabrícia C. , Costa Paulo V. C. G. , Alves-Júnior Edgar A. , Viseras César , Osajima Josy A. , Bezerra Leilson R. , de Moura Jose F. P. , de A. Silva Aline G. , Fonseca Maria G. , Silva-Filho Edson C.
Published: December 2021

The potential use of amino acids by ruminal microorganisms converting them into microbial protein for ruminants makes it challenging to supplement these nutrients in an accessible form in animals’ diets. Several strategies to protect amino acids from ruminal degradation were reported, producing amino acids available for the protein used in the intestine called “bypass.” The intercalation of biomolecules in clay mineral minerals has gained notoriety due to its ability to support, protect, transport, physicochemical properties and non-toxicity. This study aimed to investigate the incorporation of L-lysine (Lys), L-methionine (Met), and L-tryptophan (Trp) amino acids in the clay minerals sepiolite (Sep) and Veegum® (Veg) using the adsorption method. The characterization techniques of X-ray diffraction and infrared spectroscopy indicated the presence of biomolecules in the inorganic matrices. Elemental and thermal analyzes monitored the percentages of incorporated amino acids. They showed better incorporation capacities for Veg, such as Met-Veg < Lys-Veg < Trp-Veg and Lys-Sep < Met-Sep < Trp-Sep for sepiolite, except for the incorporation of Met. Matrices provide a promising alternative for planning the administration of biomolecules, using essential amino acids as models, and may offer an alternative to improve functional diet strategies.
Numerical Simulation of the Fracture Behavior of High-Performance Fiber-Reinforced Concrete by Using a Cohesive Crack-Based Inverse Analysis
Authors: Enfedaque Alejandro , Alberti Marcos G. , Gálvez Jaime C. , Cabanas Pedro
Published: December 2021

Fiber-reinforced concrete (FRC) has become an alternative for structural applications due its outstanding mechanical properties. The appearance of new types of fibres and the fibre cocktails that can be configured by mixing them has created FRC that clearly exceeds the minimum mechanical properties required in the standards. Consequently, in order to take full advantage of the contribution of the fibres in construction projects, it is of interest to have constitutive models that simulate the behaviour of the materials. This study aimed to simulate the fracture behaviour of five types of FRC, three with steel fibres, one with a combination of two types of steel fibers, and one with a combination of polyolefin fibres and two types of steel fibres, by means of an inverse analysis based on the cohesive crack approach. The results of the numerical simulations defined the softening functions of each FRC formulation and have pointed out the synergies that are created through use of fibre cocktails. The information supplied can be of help to engineers in designing structures with high-performance FRC.
Robust Lane Detection and Tracking Algorithm for Steering Assist Systems
Authors: Andrei Mihail-Alexandru , Boiangiu Costin-Anton , Tarbă Nicolae , Voncilă Mihai-Lucian
Published: December 2021

Modern vehicles rely on a multitude of sensors and cameras to both understand the environment around them and assist the driver in different situations. Lane detection is an overall process as it can be used in safety systems such as the lane departure warning system (LDWS). Lane detection may be used in steering assist systems, especially useful at night in the absence of light sources. Although developing such a system can be done simply by using global positioning system (GPS) maps, it is dependent on an internet connection or GPS signal, elements that may be absent in some locations. Because of this, such systems should also rely on computer vision algorithms. In this paper, we improve upon an existing lane detection method, by changing two distinct features, which in turn leads to better optimization and false lane marker rejection. We propose using a probabilistic Hough transform, instead of a regular one, as well as using a parallelogram region of interest (ROI), instead of a trapezoidal one. By using these two methods we obtain an increase in overall runtime of approximately 30%, as well as an increase in accuracy of up to 3%, compared to the original method.
Symmetry Methods and Conservation Laws for the Nonlinear Generalized 2D Equal-Width Partial Differential Equation of Engineering
Authors: Khalique Chaudry Masood , Plaatjie Karabo
Published: December 2021

In this work, we study the generalized 2D equal-width equation which arises in various fields of science. With the aid of numerous methods which includes Lie symmetry analysis, power series expansion and Weierstrass method, we produce closed-form solutions of this model. The exact solutions obtained are the snoidal wave, cnoidal wave, Weierstrass elliptic function, Jacobi elliptic cosine function, solitary wave and exponential function solutions. Moreover, we give a graphical representation of the obtained solutions using certain parametric values. Furthermore, the conserved vectors of the underlying equation are constructed by utilizing two approaches: the multiplier method and Noether’s theorem. The multiplier method provided us with four local conservation laws, whereas Noether’s theorem yielded five nonlocal conservation laws. The conservation laws that are constructed contain the conservation of energy and momentum.
Data Classification Methodology for Electronic Noses Using Uniform Manifold Approximation and Projection and Extreme Learning Machine
Authors: Leon-Medina Jersson X. , Parés Núria , Anaya Maribel , Tibaduiza Diego A. , Pozo Francesc
Published: December 2021

The classification and use of robust methodologies in sensor array applications of electronic noses (ENs) remain an open problem. Among the several steps used in the developed methodologies, data preprocessing improves the classification accuracy of this type of sensor. Data preprocessing methods, such as data transformation and data reduction, enable the treatment of data with anomalies, such as outliers and features, that do not provide quality information; in addition, they reduce the dimensionality of the data, thereby facilitating the tasks of a machine learning classifier. To help solve this problem, in this study, a machine learning methodology is introduced to improve signal processing and develop methodologies for classification when an EN is used. The proposed methodology involves a normalization stage to scale the data from the sensors, using both the well-known min−max approach and the more recent mean-centered unitary group scaling (MCUGS). Next, a manifold learning algorithm for data reduction is applied using uniform manifold approximation and projection (UMAP). The dimensionality of the data at the input of the classification machine is reduced, and an extreme learning machine (ELM) is used as a machine learning classifier algorithm. To validate the EN classification methodology, three datasets of ENs were used. The first dataset was composed of 3600 measurements of 6 volatile organic compounds performed by employing 16 metal-oxide gas sensors. The second dataset was composed of 235 measurements of 3 different qualities of wine, namely, high, average, and low, as evaluated by using an EN sensor array composed of 6 different sensors. The third dataset was composed of 309 measurements of 3 different gases obtained by using an EN sensor array of 2 sensors. A 5-fold cross-validation approach was used to evaluate the proposed methodology. A test set consisting of 25% of the data was used to validate the methodology with unseen data. The results showed a fully correct average classification accuracy of 1 when the MCUGS, UMAP, and ELM methods were used. Finally, the effect of changing the number of target dimensions on the reduction of the number of data was determined based on the highest average classification accuracy.
Convergence Bounds for Limited Processor Sharing Queue with Impatience for Analyzing Non-Stationary File Transfer in Wireless Network
Authors: Kochetkova Irina , Satin Yacov , Kovalev Ivan , Makeeva Elena , Chursin Alexander , Zeifman Alexander
Published: December 2021

The data transmission in wireless networks is usually analyzed under the assumption of non-stationary rates. Nevertheless, they strictly depend on the time of day, that is, the intensity of arrival and daily workload profiles confirm this fact. In this article, we consider the process of downloading a file within a single network segment and unsteady speeds—for arrivals, file sizes, and losses due to impatience. To simulate the scenario, a queuing system with elastic traffic with non-stationary intensity is used. Formulas are given for the main characteristics of the model: the probability of blocking a new user, the average number of users in service, and the queue. A method for calculating the boundaries of convergence of the model is proposed, which is based on the logarithmic norm of linear operators. The boundaries of the rate of convergence of the main limiting characteristics of the queue length process were also established. For clarity of the influence of the parameters, a numerical analysis was carried out and presented.
Novel Analysis of Hermite–Hadamard Type Integral Inequalities via Generalized Exponential Type m-Convex Functions
Authors: Tariq Muhammad , Ahmad Hijaz , Cesarano Clemente , Abu-Zinadah Hanaa , Abouelregal Ahmed E. , Askar Sameh
Published: December 2021

The theory of convexity has a rich and paramount history and has been the interest of intense research for longer than a century in mathematics. It has not just fascinating and profound outcomes in different branches of engineering and mathematical sciences, it also has plenty of uses because of its geometrical interpretation and definition. It also provides numerical quadrature rules and tools for researchers to tackle and solve a wide class of related and unrelated problems. The main focus of this paper is to introduce and explore the concept of a new family of convex functions namely generalized exponential type m-convex functions. Further, to upgrade its numerical significance, we present some of its algebraic properties. Using the newly introduced definition, we investigate the novel version of Hermite–Hadamard type integral inequality. Furthermore, we establish some integral identities, and employing these identities, we present several new Hermite–Hadamard H–H type integral inequalities for generalized exponential type m-convex functions. These new results yield some generalizations of the prior results in the literature.
Optimization Environment Definition for Beam Steering Reflectarray Antenna Design
Authors: Niccolai Alessandro , Grimaccia Francesco , Mussetta Marco , Zich Riccardo , Gandelli Alessandro
Published: December 2021

Reflectarray antennas are low-profile high-gain systems widely applied in the aerospace industry. The increase in their application is leading to the problem of getting more advanced performance while keeping the system as simple as possible. In these cases, their design cannot be conducted via analytical methods, thus evolutionary optimization algorithms are often implemented. Indeed, the design is characterized by the presence of many local minima, by high number of design variables, and by the high computational burden required to evaluate the antenna performance. The purpose of this paper is to develop, implement, and test a complete Optimization Environment that can be applied to achieve high scanning capabilities with a reflectarray. The design of the optimization environment has been selected to be flexible enough to be applied also with other different algorithms.
Novel Alkaloids from Marine Actinobacteria: Discovery and Characterization
Authors: De Rop Anne-Sofie , Rombaut Jeltien , Willems Thomas , De Graeve Marilyn , Vanhaecke Lynn , Hulpiau Paco , De Maeseneire Sofie L. , De Mol Maarten L. , Soetaert Wim K.
Published: December 2021

The marine environment is an excellent resource for natural products with therapeutic potential. Its microbial inhabitants, often associated with other marine organisms, are specialized in the synthesis of bioactive secondary metabolites. Similar to their terrestrial counterparts, marine Actinobacteria are a prevalent source of these natural products. Here, we discuss 77 newly discovered alkaloids produced by such marine Actinobacteria between 2017 and mid-2021, as well as the strategies employed in their elucidation. While 12 different classes of alkaloids were unraveled, indoles, diketopiperazines, glutarimides, indolizidines, and pyrroles were most dominant. Discoveries were mainly based on experimental approaches where microbial extracts were analyzed in relation to novel compounds. Although such experimental procedures have proven useful in the past, the methodologies need adaptations to limit the chance of compound rediscovery. On the other hand, genome mining provides a different angle for natural product discovery. While the technology is still relatively young compared to experimental screening, significant improvement has been made in recent years. Together with synthetic biology tools, both genome mining and extract screening provide excellent opportunities for continued drug discovery from marine Actinobacteria.
Fucoidan Independently Enhances Activity in Human Immune Cells and Has a Cytostatic Effect on Prostate Cancer Cells in the Presence of Nivolumab
Authors: Park Ah Young , Nafia Imane , Stringer Damien N. , Karpiniec Samuel S. , Fitton J. Helen
Published: December 2021

Fucoidan compounds may increase immune activity and are known to have cancer inhibitory effects in vitro and in vivo. In this study, we aimed to investigate the effect of fucoidan compounds on ex vivo human peripheral blood mononuclear cells (PBMCs), and to determine their cancer cell killing activity both solely, and in combination with an immune-checkpoint inhibitor drug, Nivolumab. Proliferation of PBMCs and interferon gamma (IFNγ) release were assessed in the presence of fucoidan compounds extracted from Fucus vesiculosus, Undaria pinnatifida and Macrocystis pyrifera. Total cell numbers and cell killing activity were assessed using a hormone resistant prostate cancer cell line, PC3. All fucoidan compounds activated PBMCs, and increased the effects of Nivolumab. All fucoidan compounds had significant direct cytostatic effects on PC3 cells, reducing cancer cell numbers, and PBMCs exhibited cell killing activity as measured by apoptosis. However, there was no fucoidan mediated increase in the cell killing activity. In conclusion, fucoidan compounds promoted proliferation and activity of PBMCs and added to the effects of Nivolumab. Fucoidan compounds all had a direct cytostatic effect on PC3 cells, as shown through their proliferation reduction, while their killing was not increased.
Fucoxanthin Pretreatment Ameliorates Visible Light-Induced Phagocytosis Disruption of RPE Cells under a Lipid-Rich Environment via the Nrf2 Pathway
Authors: Liu Yunjun , Guo Zixin , Wang Shengnan , Liu Yixiang , Wei Ying
Published: December 2021

Fucoxanthin, a special xanthophyll derived from marine algae, has increasingly attracted attention due to its diverse biological functions. However, reports on its ocular benefits are still limited. In this work, the ameliorative effect of fucoxanthin on visible light and lipid peroxidation-induced phagocytosis disruption in retinal pigment epithelium (RPE) cells was investigated in vitro. Marked oxidative stress, inflammation, and phagocytosis disruption were evident in differentiated RPE cells following their exposure to visible light under a docosahexaenoic acid (DHA)-rich environment. Following pretreatment with fucoxanthin, however, the activated nuclear factor erythroid-derived-2-like 2 (Nrf2) signaling pathway was observed and, furthermore, when the fucoxanthin -pretreated RPE cells were irradiated with visible light, intracellular reactive oxygen species (ROS), malondialdehyde (MDA) levels and inflammation were obviously suppressed, while phagocytosis was significantly improved. However, following the addition of Nrf2 inhibitor ML385, the fucoxanthin exhibited no ameliorative effects on the oxidative stress, inflammation, and phagocytosis disruption in the RPE cells, thus indicating that the ameliorative effect of fucoxanthin on the phagocytosis of RPE cells is closely related to the Nrf2 signaling pathway. In conclusion, these results suggest that fucoxanthin supplementation might be beneficial to the prevention of visible light-induced retinal injury.
Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in Live Phaeodactylum tricornutum Cultures
Authors: Macdonald Miller Sean , Abbriano Raffaela M. , Segecova Anna , Herdean Andrei , Ralph Peter J. , Pernice Mathieu
Published: December 2021

Microalgal biotechnology shows considerable promise as a sustainable contributor to a broad range of industrial avenues. The field is however limited by processing methods that have commonly hindered the progress of high throughput screening, and consequently development of improved microalgal strains. We tested various microplate reader and flow cytometer methods for monitoring the commercially relevant pigment fucoxanthin in the marine diatom Phaeodactylum tricornutum. Based on accuracy and flexibility, we chose one described previously to adapt to live culture samples using a microplate reader and achieved a high correlation to HPLC (R2 = 0.849), effectively removing the need for solvent extraction. This was achieved by using new absorbance spectra inputs, reducing the detectable pigment library and changing pathlength values for the spectral deconvolution method in microplate reader format. Adaptation to 384-well microplates and removal of the need to equalize cultures by density further increased the screening rate. This work is of primary interest to projects requiring detection of biological pigments, and could theoretically be extended to other organisms and pigments of interest, improving the viability of microalgae biotechnology as a contributor to sustainable industry.
Rehabilitation of the Completely Edentulous Mandible by All-on-Four Treatment Concept: A Retrospective Cohort Study with Up to 10 Years Follow-Up
Authors: Grandi Tommaso , Signorini Luca
Published: December 2021

(1) Background and Objectives. Currently, there are no definitive long-term data about clinically significant difference in the failure of prosthesis and implant or marginal bone loss related to the rehabilitation of the completely edentulous mandible by all-on-four treatment concept. The main aim of present investigation was to report the long-term outcomes (10-years follow-up) of complete-arch mandibular rehabilitations based on the all-on-four concept. (2) Materials and Methods. Patients in need of extractions of teeth due to the occurrence of caries and/or severe periodontal disease and patients presented with edentulous mandibles were enrolled to the study. A total of 96 participants (mean follow-up period after intervention of 3185.2 days) were enrolled in the study. Participants were evaluated at the first visit, 10 days after intervention and every year after the intervention. Implant and prosthesis survival, bone loss and both local biological and mechanical complications were evaluated during the follow-up period. (3) Results. An implants’ survival rate of 97.9% was observed at the end of the follow-up period. Biological complications were reported in 19.8% of patients, whereas mechanical complications were reported in 27.1% of cases. The average marginal bone level at baseline was −0.03 mm. A significant marginal bone loss was observed after 10-years follow-up (2.5 mm). Binary logistic regression analysis showed significant association between smoke and both marginal bone loss and local biological complications. Lastly, a significant association was observed between bruxism and mechanical complications. (4) Conclusions. The high implant and prosthesis survival rate and the moderate incidence of biological and mechanical complications observed in present investigation can be associated to several factors such as high implant primary stability, prosthetic design, and control of the occlusal forces.
Glycemic Control and Management in Pharmacist-Led Diabetic Clinic vs. Physician-Led Diabetic Clinic
Authors: Alghadeer Sultan M. , Alsuwayni Bashayr , Almuwayjid Abdulmohsen K. , Almadi Mohammed S. , Mubarak Abdullah M. , Khunayn Rawan M. bin , Al-Arifi Mohamed N.
Published: December 2021

Background and Objectives: Globally, diabetes Mellitus (DM) is a life-threatening disease that, if it remains uncontrolled, can lead to mortality or serious complications. Despite the noticeable benefits of clinical pharmacist in managing diabetes, some institutions in Saudi Arabia are reluctant to establish a pharmacist-led diabetic clinic for monitoring and follow-up. The objective of this study is to assess the glycemic control by comparing the reduction in hemoglobin A1c (HbA1c) percentage between patients followed in the pharmacist-led diabetic clinics vs. those followed in physician-led diabetic clinics. Materials and Methods: A retrospective observational study with a 12-month follow-up were used to detect the difference in the glycemic control by comparing the reduction in HbA1c percentage from the baseline, and average changes in HbA1c, fasting blood glucose (FBG), blood pressure (BP), and lipid panel between the two groups. The level of self-care was assessed by Summary of Diabetes Self-Care Activities (SDSCA) Questionnaire. Results: The study involved 52 patients who visited the diabetic clinic at a community teaching hospital. Exactly 24 patients were followed by the pharmacist-led diabetic clinics, while 28 were followed by physician-led diabetic clinics. HbA1c baseline was 8.7% and 8.4% for pharmacist and physician, respectively. The average difference in HbA1c for the pharmacist-led diabetic clinics vs. the physician-led diabetic clinics was not statistically significant (8.67 vs. 8.56; p = 0.77). Moreover, no difference in the glucose profile, lipid panel, and blood pressure were seen between the two groups. However, the median HbA1c change from baseline between the two groups significantly favored the pharmacist-led clinic (0.7 vs. 0.003; p = 0.04).The average of responses in all four aspects of the SDSCA (diet, exercise, blood sugar testing, and foot care) was also higher among patients in the pharmacist-led diabetic clinic. Conclusions: Pharmacist-led diabetic clinics for glycemic control and follow-up showed efficient results that encourage the comprehensive and integral inter-professional patient care.
Impaired Folate-Mediated One-Carbon Metabolism in Type 2 Diabetes, Late-Onset Alzheimer’s Disease and Long COVID
Authors: Hayden Melvin , Tyagi Suresh
Published: December 2021

Impaired folate-mediated one-carbon metabolism (FOCM) is associated with many pathologies and developmental abnormalities. FOCM is a metabolic network of interdependent biosynthetic pathways that is known to be compartmentalized in the cytoplasm, mitochondria and nucleus. Currently, the biochemical mechanisms and causal metabolic pathways responsible for the initiation and/or progression of folate-associated pathologies have yet to be fully established. This review specifically examines the role of impaired FOCM in type 2 diabetes mellitus, Alzheimer’s disease and the emerging Long COVID/post-acute sequelae of SARS-CoV-2 (PASC). Importantly, elevated homocysteine may be considered a biomarker for impaired FOCM, which is known to result in increased oxidative–redox stress. Therefore, the incorporation of hyperhomocysteinemia will be discussed in relation to impaired FOCM in each of the previously listed clinical diseases. This review is intended to fill gaps in knowledge associated with these clinical diseases and impaired FOCM. Additionally, some of the therapeutics will be discussed at this early time point in studying impaired FOCM in each of the above clinical disease states. It is hoped that this review will allow the reader to better understand the role of FOCM in the development and treatment of clinical disease states that may be associated with impaired FOCM and how to restore a more normal functional role for FOCM through improved nutrition and/or restoring the essential water-soluble B vitamins through oral supplementation
Arab Women Adherence to the Mediterranean Diet and Insomnia
Authors: Zaidalkilani Ayah T. , Alhaj Omar A. , Serag El-Dine Mohamed F. , Fekih-Romdhane Feten , AlRasheed Maha M. , Jahrami Haitham A. , Bragazzi Nicola L.
Published: December 2021

Background and objective: Sleeping difficulties affect the overall health, nutrition, and wellbeing. The Mediterranean diet has proven effective in improving the quality of life and overall health of people of all ages. Therefore, this study aimed to determine whether adherence to the Mediterranean diet (MD) is linked to reduced insomnia in Arabic-speaking female adults in Jordan. Materials and Methods: A self-administered, cross-sectional survey was used to detect the relationship between MD and sleep quality in Arabic-speaking female adults. Data were collected from 917 Arabic-speaking female participants of 14 Arab nationalities in Jordan between March and May 2021 via social media. All participants answered the whole questionnaire, including questions on sociodemographic aspects, Mediterranean diet adherence, sleeping habits measured with the Athens Insomnia Scale (AIS), and lifestyle components such as smoking and dietary patterns. Results: The mean age of the 917 Arabic-speaking female participants was 36 ± 10 years. Most participants were unemployed (85%) single females (64%) with an undergraduate degree (74%). Most of them (86%) were non-smokers. More than half of the participants were Jordanians (57%). The BMI was normal for 52% of the participants, whereas 26% of them were overweight, and 12% were obese. One-way ANCOVA showed a statistically significant difference between MD adherence score categories and AIS, F (2, 914) = 3.36, p = 0.015. Among the MD adherence score categories, we found that between groups, MD scores above or equal to 10 were associated with a statistically significant difference in AIS. Cohen’s value was calculated for the three MD score categories and indicated a ‘small’ effect size association between all adherence scores of the MD categories and AIS. Conclusions: In conclusion, our findings provide preliminary evidence that participants’ adherence to the MD was significantly associated with better sleep and reduced insomnia symptoms, highlighting the need for further research.
Prediction of Upper Surface Roughness in Laser Powder Bed Fusion
Authors: Wang Wenjia , Garmestani Hamid , Liang Steven Y.
Published: December 2021

In this study, a physics-based analytical method was proposed for the prediction of upper surface roughness in laser powder bed fusion (LPBF). The temperature distribution and molten pool shape in the melting process were first predicted by an analytical thermal model. The cap area of the solidified molten pool was assumed to be half-elliptical. Based on this assumption and the principle of mass conservation, the cap height and the specific profile of the cap area were obtained. The transverse overlapping pattern of adjacent molten pools of upper layer was then obtained, with given hatch space. The analytical expression of the top surface profile was obtained after putting this overlapping pattern into a 2D coordinate system. The expression of surface roughness was then derived as an explicit function of the process parameters and material properties, based on the definition of surface roughness (Ra) in the sense of an arithmetic average. The predictions of surface roughness were then compared with experimental measurements of 316L stainless steel for validation and show acceptable agreement. In addition, the proposed model does not rely on numerical iterations, which ensures its low computational cost. Thus, the proposed analytical method can help understand the causes for roughness in LPBF and guide the optimization of process conditions to fabricate products with good quality. The sensitivity of surface roughness to process conditions was also investigated in this study.