bluuebunny/crossref_metadata_2025 huggingface dataset:
THE ROLE OF A HIGHER EDUCATION DIPLOMA IN THE PROFESSIONALCAREER OF THE SPECIALIST IN THE FUTURE
Authors: Kolesnikova Kateryna V. , Lukianov Dmytro V. , Olekh Tatyana M.
Published: April 2020

The field of information technology is extremely attractive in terms of job search. What do you need to be a specialist in this field? The information technology industry was the first to accept the fact that the ability to confirm the possession of knowledge, skills, and abilities in practice by a particular employee is higher than the presence of such an employee with an education document. This path also proved to be the fastest path for acquiring qualified personnel at the stage of the rapid development of the industry. There are more and more positions where experience is required, and not a specialized diploma. How characteristic are these trends for other areas of life? What is this? Is this step into the future or a tribute to modern trends? New technologies, new conditions, new business opportunities give rise to new knowledge and even new professions. There are no specialists in them, they will be trained in practice and practice will be their specialized education. Over time, universities and graduates will appear. But as life shows, it is precisely those who started without a diploma who will create new requirements and standards of professional activity the proposed research examines the current state of affairs in the field of education. The authors propose to analyze current trends in the labor market, to study some tools and training models. The question of the possibility of making a career without profile education is being considered. Can the holders of degrees and titles in a classical university provide the level of knowledge that employers need today? How relevant is the crea-tion of corporate universities for the training and professional development of employees? According to surveys, more than 54 % of employ-ers are willing to employ an employee without a diploma. Why? The quality of knowledge obtained at a university does not always meet themodern requirements of not only the information technology market but also just the labor market. However, at different times, companiesand candidates have put different meanings in the definition of “good”. The task of the university is to indicate the main reference pointsfrom which to build on, continuing to study independently throughout life. Therefore, obtaining a document on graduation is only the begin-ning of a career path. Having a diploma does not immediately allow you to get a well-paid job and implies further independent training andthe acquisition of practical experience.
Application of Civil Law Theory In the Termination of Custody of Adopted Children in Indonesia
Authors: Rezki Annissa , Anggraeni RR Dewi , Yunus Nur Rohim
Published: April 2020

Abstract:Today there are so many couples who have long been married, but have not been blessed with a child in married life. In Indonesia, so many believe that one of the efforts to get a child is by inducement, which is presenting a foster child to give a provocation to a couple who do not have children to be immediately given offspring. Most of them raised their own family, others lifted children from other people who are not family, and even raised children from orphanages whose origins are unclear. From this the legal protection of the adopted child is needed in order to obtain guarantees and a decent life. This study will discuss how legal protection, the criteria for terminating custody of adopted children, and inheritance rights will be obtained. This research uses descriptive qualitative method with a normative approach.Keywords: foster children, child protection, civil lawAbstrak:Zaman sekarang begitu banyaknya pasangan yang sudah lama menikah, namun belum dikaruniai seorang anak dalam kehidupan berumah tangga. Di Indonesia begitu banyak yang percaya bahwa salah satu upaya untuk mendapatkan seorang anak adalah dengan pancingan, yaitu menghadirkan seorang anak asuh untuk memberikan pancingan kepada pasangan yang belum memiliki anak agar segera diberikan keturunan. Sebagian besar ada yang mengangkat dari pihak keluarga sendiri, ada juga yang mengangkat anak dari orang lain yang bukan keluarga, bahkan mengangkat  anak dari panti asuhan yang tidak jelas asal usulnya. Dari hal tersebut diperlukan perlindungan hukum terhadap anak yang diangkat tersebut agar mendapat jaminan dan kehidupan yang layak. Dalam penelitian ini akan dibahas bagaimana perlindungan hukum, kriteria pemutusan hak asuh anak angkat, dan hak waris yang akan didapatkan. Penelitian ini menggunakan metode kualitatif deskriptif dengan pendekatan normatif.Kata kunci : anak asuh, perlindungan anak, hukum perdata
Морфофункціональні особливості Pinus sylvestris L. у лісостанах борів Малого Полісся
Authors: Zaika Volodymyr , Rudenko Anatoliy
Published: April 2020

Досліджували формування та морфофізіологічні особливості функціонування 27-145-річних соснових деревостанів природного насінного походження, які ростуть в сухих, свіжих, вологих, сирих і мокрих борах Малого Полісся. Встановлено, що Pinus sylvestris L. в борах Малого Полісся росте за V-І класами бонітету. Переважно тут формуються чисті соснові деревостани. Однак у складі близько половини деревостанів трапляється також Betula pendula Roth., частка якої коливається від поодиноких екземплярів до 10%. Вона характеризується істотним відставання за ростом від сосни, яке за висотою становить 22,1-78,9%, а за діаметром – 17,4-70,1%. Повнота деревостанів природного походження, не залежно від віку, коливається в межах 0,50-0,93.

Найкращі умови для росту і функціонування P. sylvestris серед борових типів лісу складаються в свіжих і вологих борах. У цих умовах сосна характеризується найвищою продуктивністю. Вона формує хвою, яка за морфолого-анатомічними показниками та вмістом пластидних пігментів найбільшою мірою наближена до генетично-зумовленого рівня. В сирих борах виявлено істотне погіршення стану P. sylvestris, яке проявляється у зниженні інтенсивності росту дерев, зменшенні морфолого-анатомічних показників хвої та біосинтезу зелених і жовтих пігментів. Встановлено, що серед молодняків істотно вищими середньоденними показниками біоелектричних потенціалів характеризується сосна в сухих і свіжих борах. У середньовікових і пристиглих деревостанах виявлено істотне зниження біоелектричної активності сосни в сухих і свіжих борах та зростання – у вологих, сирих і мокрих. У молодняків, середньовікових і пристиглих деревостанів простежується достатньо чітке зростання показників імпедансу і зменшення поляризаційної ємності зі збільшенням вологості ґрунту. Найменш сприятливі умови для життєдіяльності P. sylvestris складаються в мокрому борі. Найбільш чутливими до зміни умов зволоження в борах виявились довжина, площа перетину і поверхні хвої, вміст хлорофілів і діелектричні показники.
Silver birch health condition in the parks of Kharkiv National Agrarian University named after V.V. Dokuchaev
Authors: Meshkova Valentyna L. , Koshelyaeva Yana , Kolienkina Maryna S.
Published: April 2020

Recently health condition of silver birch (Betula pendula Roth.) stands worsens in the forest and ornamental stands. It is important to know the probability of its improvement or deterioration in particular stands to take the necessary measures in time.

The aim of the research was to assess the trends in the health condition dynamics of silver birch stands depending on their age class and the initial health condition of the trees. The research was carried out in 2015-2019 in the Silver birch linear stands in two parks of Kharkiv National Agrarian University named after V.V. Dokuchaev (49º53' N, 36º27' E). Five sample plots of the 3th age class are located in Veterans Park and the other 4 sample plots of the 5rd age class – in Arboretum of this University.

For each inspected tree diameter at breast height (DBH) was measured in 2015 and 2019. Crown defoliation and health condition class were visually assessed in July of each year. Health condition index (HCI) was calculated as mean weighted from trees number of each category of health condition, separately for all living and dead trees (HCI1–6) and for living trees only (HCI1–4). Tree mortality was expressed as a percentage of lost (dead) trees for research period out of the total trees in 2015.

It was shown that the silver birch stands of the 3rd age class didn't change their health condition for 2015-2019 or improved it. The stands of the 5th age class worsened their health condition in three sample plots and improved it in one sample plot. Within each age class, the stands of lower diameter class had a worse health condition. Tree mortality was registered in two out of five sample plots in the stands of the 3rd age class and in three out of four sample plots in the stands of the 5th age class. In the stands of the 5th age class the trees from the stands of the smallest diameter class characterized by the highest mortality level (22.4%) and the worse health condition (HCI1-4 =2.8).

In pooled sample of plots, the death probability for silver birch trees, which 4 years ago had the 1st class of health condition is 3.5%, those of the 2nd class have the death probability of 10.7%, the trees of the 3rd class – 36.9%, those of the 4th class – 84.6%. As for an initially weakened stand, its trees which 4 years ago had the 1st, the 2nd, the 3rd or the 4th health condition class, have the death probability of 5%, 18.5%, 33.3%, and 100% respectively.

Thus, the weakened silver birch stand which contains trees of 1st - 3rd classes of health condition is able to restore condition to a healthy one, and the deterioration may be expected only for severely weakened trees (having 3rd class of health condition in 2015). The weakened silver birch stand which has the trees of 1st - 4th health condition classes is most likely to weaken even more severely over 4 years.
To Act or not to Act . Białowieża. Forest under Conflicting Ecological Paradigms
Authors: Von Gadow Klaus , Cieszewski Chris J. , Kershaw Jr. John A.
Published: April 2020

Białowieża, a national treasure to the people of Poland and a unique ecosystem of historical significance, has become a site of an intense international debate, following a large scale Bark Beetle outbreak. The controversy centers around two opposing ecological paradigms: 1). Ecosystems without human interference would eventually reach a climax state that is self-regulating and in equilibrium (The No Action paradigm); 2). Trees infected by Ips typographus should be salvaged in order to reduce CO2 emissions, lower the fire risk, and prevent the collapse of healthy tree communities (The Adaptive Action paradigm).

This contribution analyzes historical evidence and recent issues related to the management of Poland’s Białowieża Forest and presents a proposal towards a negotiated landscape design that allows for small-scale spatial mixing of different land-uses. Białowieża can become a unique example of a continuously evolving forest landscape that is resilient to fluctuating environmental conditions, human values and societal demands.
Моделювання процесу енергетичного переходу методом системної динаміки: енергетичні кооперативи як інструмент переходу до сталого використання деревної біомаси в житловому секторі
Authors: Zahvoyska Lyudmyla , Bletska Olha
Published: April 2020

Домінуюча парадигма енергогенерування централізована, високовуглецева і породжує енергетичну бідність. Імператив глобальної зміни клімату пріоритезує процес енергетичного переходу, одним із аспектів якого є стале енергетичне використання деревної біомаси. Обґрунтовано необхідність трансформації енергетичного сектора шляхом використання холістичних методів дослідження і деліберативних методів прийняття рішень. Побудовано причинно-наслідкову модель процесу енергетичного переходу і потокову модель поширення екоінновацій для процесу формування енергетичних кооперативів. За результатами регресійного аналізу макроекономічних показників глобальної конкурентоспроможності країн виконано параметризацію потокової моделі для умов України. За результатами моделювання, проведеного з використанням цієї моделі, встановлено, що процес формування енергетичних кооперативів відбуватиметься повільно, насамперед – через неефективність інституцій. Однак відповідно до здійсненого нами опитування в м. Борислав встановлено, що 18,1 % респондентів знайомі з концепцією енергетичних кооперативів, з яких кожен п’ятий готовий вкласти власні кошти в їхній розвиток, близько половини опитаних бажають змінити системи опалення в своїх помешканнях, що значно простіше зробити членам кооперативів. Використовуючи причинно-наслідкову модель, протестовано запропоновані нами SWOT-стратегії процесу покращення енергетичного використання деревної біомаси в житловому секторі, визначено додаткові важелі впливу, виявлено потенційні небажані наслідки і джерела непевності, що сприятиме глибшому розумінню і кращому управлінню процесом енергетичного переходу.
Síndrome MELAS en pediatría. Reporte de caso
Authors: López-Pérez Juan José , Galán-Gutiérrez Gloria Mercedes , Luna-Luna Manuel Alejandro , Lancheros-Delgadillo Diocel Orlando
Published: April 2020

Introducción. El síndrome de encefalopatía mitocondrial, acidosis láctica y episodios similares a un accidente cerebrovascular (MELAS, por su sigla en inglés) es la enfermedad mitocondrial más frecuente. Estas patologías se caracterizan por ser hereditarias, multisistémicas y progresivas, y por causar un compromiso predominantemente neurológico que provoca discapacidad y mortalidad, por lo que el diagnóstico temprano y la consejería genética son de gran importancia para mejorar el pronóstico de estos pacientes.Presentación del caso. Paciente femenina de cinco años quien fue llevada a consulta al servicio de pediatría por crisis epiléptica, retraso psicomotor y ataxia. Aunque en la consulta inicial sus estudios de neuroimagen fueron normales, se observó hiperlactatemia. Además, se encontró una relación lactato/piruvato >20, por lo que se sospechó enfermedad mitocondrial.Seis meses después, la paciente presentó deterioro progresivo. Se realizó tomografía axial computarizada de cráneo y resonancia magnética por espectroscopia que permitieron identificar una lesión isquémica occipital y aumento del lactato cerebral, respectivamente. Para confirmar el diagnóstico de síndrome MELAS, se solicitó estudio de ADN mitocondrial, en el que se observó la mutación m.3243A>G. La paciente tuvo un rápido deterioro, presentando una involución de las capacidades adquiridas, y falleció a los cuatro años del inicio de los signos clínicos.Conclusión. Las enfermedades mitocondriales deben ser consideradas en pacientes con antecedentes de epilepsia y otras alteraciones neurológicas como ataxia e involución del neurodesarrollo.
El periodo refractario de las células excitables: ¿inconsistencias de un dogma?
Authors: Barco-Ríos John , Duque-Parra Jorge Eduardo , Barco-Cano Johanna Alexandra
Published: April 2020

Introducción. Los canales activados por voltaje para Na+ y para K+ presentan compuertas de activación e inactivación, las cuales se abren y se cierran dependiendo de la intensidad de la corriente eléctrica que fluye por la membrana cuando está respondiendo a un estímulo. Durante este breve momento, la membrana entra en un periodo de refractariedad que la hace insensible a otros estímulos.Objetivo. Demostrar que los periodos refractarios absoluto y relativo se presentan a medida que se va desarrollando el potencial de acción y no después de que se ha completado, mediante un análisis teórico basado en el funcionamiento eléctrico normal de los canales activados por voltaje para Na+ y K+.Cuestionamientos. En diversos textos y artículos de fisiología, las definiciones de los periodos refractarios absoluto y relativo son confusas y erróneas, puesto que no tienen en cuenta el funcionamiento normal de los canales activados por voltaje. Además, la ubicación que dan a dichos periodos con respecto al potencial de acción es desfasada y su tiempo de duración es incierto.Conclusión. Los periodos refractarios absoluto y relativo se presentan durante el desarrollo del potencial de acción y no después de que ha sido completado.
Pediculosis capitis y transmisión potencial de enfermedades infecciosas reemergentes en Colombia. Revisión de la literatura
Authors: López-Valencia David , Medina-Ortega Ángela Patricia , Hoyos-Samboní Diego Fernando , Salguero Carolina , Vásquez-Arteaga Luis Reinel
Published: April 2020

Introducción. La infestación por piojos de la cabeza en humanos (Pediculus humanus capitis) se conoce como pediculosis capitis. Estos parásitos pueden ser vectores de enfermedades infecciosas con potencial reemergente.Objetivos. Revisar la literatura actual sobre las enfermedades infecciosas transmitidas por piojos de la cabeza y realizar una breve descripción de sus manifestaciones clínicas.Materiales y métodos. Se realizó una revisión de la literatura en PubMed, ScienceDirect, SciELO y Redalyc mediante la siguiente estrategia de búsqueda: años de publicación: 1938 a 2019; idioma: inglés y español; términos de búsqueda: “Pediculus”, “lice infestations”, “bacteria”, “emerging communicable diseases”, “Rickettsia”, “Bartonella”, “Borrelia”, “Acinetobacter”, “Yersinia“, and “Colombia”, y sus equivalentes en español.Resultados. De los 110 artículos incluidos, la mayoría correspondió a investigaciones originales (48.2%). A nivel mundial, numerosos estudios han reportado la transmisión de Rickettsia prowazekii, Bartonella quintana, Borrelia recurrentis, Staphylococcus aureus, Acinetobacter baumannii y Yersinia pestis, entre otras bacterias, por los piojos de la cabeza y del cuerpo en humanos.Conclusiones. Aunque las enfermedades infecciosas transmitidas por piojos de la cabeza son raras, guardan potencial reemergente en poblaciones afectadas por migraciones humanas, crisis sociopolíticas, indigencia e inmunosupresión. En Colombia no se han realizado investigaciones sobre la transmisión de estas enfermedades por Pediculus spp., por lo que se sugiere que en estudios futuros se determine la prevalencia y los aspectos epidemiológicos de las enfermedades transmitidas por piojos de la cabeza en población colombiana.
First record of the Lesser Long-nosed Bat, Leptonycteris yerbabuenae Martinez & Villa-R., 1940 (Chiroptera, Phyllostomidae), in Nicaragua
Authors: Saldaña Tapia Octavio A. , Ñamendy Milton , Martínez-Fonseca José G.
Published: April 2020

We report the first record of the Lesser long-nosed Bat, Leptonycteris yerbabuenae Martinez & Villa-R., 1940 in Nicaragua based on a specimen from San Nicolas, Estelí Department, north-central Nicaragua. The new record extends the known range of this large, migratory, nectar-feeding species 100 km southeast from the closest previous record in western Honduras.
Synthesis, characterization and antimicrobial studies of new chiral amide-Schiff base derivatives
Authors: YÜKSEKDANACI Seda , ASTLEY Demet , YAŞA İhsan
Published: April 2020

In this study, new chiral Schiff bases (3-9) obtained from the reaction of chiral amide derivatives with different substituted aromatic aldehyde were synthesized. All synthesized compounds structures were characterized by FTIR, 1H-NMR, 13C-NMR and elemental analysis. All of the new synthesized compounds were tested in vitro against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella thyphimirium, Klebsiella pneumoniae, Enterococcus faecalis, Pseudomonas aeruginosa, Candida albicans for their antibacterial and antifungal activities. All compounds have shown moderate to good antibacterial activity against Gram-positive and Gram-negative bacteria.  Among the compounds tested compound 7 showed most potent inhibitory action against test organisms.
A HILIC stationary phase fuctionalized with glutathione by thiol-ene chemistry on monodisperse-porous polymer microparticles
Authors: KİP Cigdem
Published: April 2020

Monodisperse-porous
microparticles functionalized a with a zwitterionic ligand were synthesized as
a new stationary phase for hydrophilic interaction chromatography.
Monodisperse-porous poly(3-trimethoxysilylpropyl methacrylate-co-ethylene
dimethacrylate), poly(TMSPM-co-EDMA) microspheres (6 μm in size) were obtained
with different seed latex/monomer ratios and diluent compositions by multistage
microsuspension copolymerization. The zwitterionic chromatographic ligand
containing thiol moiety (i.e. glutathione) were covalently attached onto the
TMSPM attached-poly(TMSPM-co-EDMA) microparticles. The selected starting
material allowed the direct attachment of zwitterionic ligand onto the support material using thiol-ene chemistry.
The derivatized microparticles were slurry packed into the microbore columns
with 2 mm i.d. and used as stationary phase for the separation of nucleosides
in hydrophilic interaction chromatography with the plate numbers up to 54.000
plates/m. The results showed the usability 
of  tailored poly (TMSPM-co-EDMA)
microparticles as a stationary phase and thiol-ene chemistry in the manufacture
of a chromatographic stationary phase with high efficiency in hydrophilic liquid
chromatography applications.
DOĞU KARADENİZ BÖLGESİ GEÇ DEMİR ÇAĞI KÜLTÜRÜNE AİT YENİ ARKEOLOJİK BULGULAR VE KARADİKMEN KALESİ
Authors: KAYMAKÇI Salih
Published: April 2020

2015 yılında başladığımız ve bugüne kadar sürdürdüğümüz Doğu Karadeniz Bölgesi yüzey araştırmalarımızda Giresun İli ve ilçelerinde Geç Demir Çağı çanak-çömleği görülen az sayıda yerleşme yeri tespit ettik. Giresun ilinin dağlık kesimindeki Çamoluk ilçesinde 2017 yılı araştırmalarımızda tespit ettiğimiz Karadikmen Kale Yerleşimi (Giresun ili dağlık kesim) üzerinde gördüğümüz bir grup çanak çömlek kırmızı boya bezemeli, teknik ve form bakımından, tanıdığımız Geç Demir Çağı keramik özelliklerini taşımaktaydı. Geç Demir Çağı’na tarihlediğimiz Anadolu Demir Çağ Keramiği özelliği taşıyan açkılı astar üzerine yapılan bitkisel, band ve geometrik bezemelerden oluşan bu keramik grubunun bölgenin Demir Çağ sınırlarını yeniden belirlemeye yönelik çalışmalar açısından önemli olacağı düşüncesindeyiz. Bölgede Tunç Çağları’nın ne zaman bittiği, Demir Çağı’nın ne zaman ve hangi şartlarda başladığı belirgin değildir. Çalışma sahamızın Demir Çağı kültürlerini bölgenin yakınında daha önce Çoruh ve Kelkit Havzası’nda yapılan çalışmalar ile karşılaştırdık. Bölgede yapılan araştırmalar sonucunda Doğu Karadeniz Bölgesi’nin güney hinterlandını oluşturan Kelkit Havzası’nın doğu bölümünde Demir Çağı’na ait yerleşmelerin olduğu, fakat bölge ile ilgili yazılı belgelerin hala yetersiz kaldığını söyleyebiliriz.
ADLANDIRMA, TOPONOMİ VE ANADOLU’NUN TÜRKLEŞMESİNDEKİ ADLANDIRMA GELENEĞİ
Authors: ARİFOĞLU Yunus
Published: April 2020

Adlandırma insanların bir şeye anlam yüklemeleriyle başlamıştır. İnsan adlandırma ile şeyleri bilmiştir. Aynı zamaanda insan, adlandırm ile mana yönünden oluşan boşluğunu gidermiştir. Çünkü insan yeryüzüne düştüğünde yabancısı olduğu yere alışmak için tanımlama ihtiyacıyla anlamlandırmaya gitmiştir. Yer adlarını Toponomi ilim dalı incelemektedir. Bu bilim dalı adlandırmayı incelerken, hem diğer bilim dallarından hem de kendi alt bilim dallarından yararlanmaktadır. Kadim mirasın zenginliği toponomi ile açıklanıp yorumlanabilmektedir. Bu sayede bir yöre tarihi bağlamında değerli bilgilere vakıf olunabilmektedir.
Adlandırmanın hangi itkiyle verildiği pek bilinemese de, adlandırırkenki faktörler bilinebilmektedir. Yer adları verilirken en önemli faktörlerden birisi doğadır. Adlandırmadaki diğer faktör insandır. Toplumsallaşma, renkler ve daha benzeri faktörler de adlandırmada etkili olmuşlardır.
Her millet mekanı kendi anlayabileceği şekilde adlandırmakta ve göç ettiği coğrafyalardaki birikimden de istifade etmektedir. Ortaçağ’da yoğun Türk nüfusunun Anadolu’da yerleşmesiyle Anadolu bir Müslüman Türk coğrafyası haline gelmiştir. Anadolu’ya gelen Türkler Oğuz soyundan gelenlerdir. Anadolu’ya rengini onlar vermişlerdir. Anadolu’nun Türk yurdu haline gelmesiyle birlikte Türkler Anadolu coğrafyasındaki adları Türk Diline uyarlamış veya yeniden adlandırmışlardır. Türk ad verme kendisine has bazı etkiler taşımaktadır. Farklı coğrafyalara dağılan Türkler, çeşitli milletler ve onların dilleriyle ilişkide olmuşlardır. Bu durum Türklerin ad vermelerine etki etmiştir.
Bu çalışmada yer adlarını inceleyen toponomi izah edilmiş ve adlandırmanın hikâyesi incelenmiştir. Adlandırmanın kaynağı ve buna etki eden faktörler ele alınmıştır. Ardından Türklerin Anadolu’da ad vermedeki usülleri incelenmiştir. Bu çalışmada, yer adları bağlamında oluşan modern literatürden istifade edilmiştir.
THE IMPACT OF THE FOLK FAIRY TALES ON THE EARLY STAGE OF A CHILD DEVELOPMENT
Authors: MIRAZANASHVILI Nodar , QURDADZE Paata
Published: April 2020

The given article considers negative assessment of the scholarly approaches to the fairy tales all along the post-Soviet space (with particular emphasis on Georgia).
The authors review the relevant literature focused on historical aspects of creation of the fairy tales, in particular the folk fairy tales.
Analysis of the causes of emergence of the fairy tales is accentuating their priority role in children’s education and upbringing. The national folk fairy tale alongside with number of educational measures is a crucial component in a long and comprehensive process of forming a child personality as well as development of necessary skills. The fairy tales not only broaden the consciousness of children but extend utmost influence on their subconscious world.
The article contains a number of recommendations to parents on how to use the fairy tales in bringing up their children. The recommendations also refer to the contents and type of the fairy tales that should be told to children and when it is more appropriate to engage them in narration.
Telling stories before falling asleep is compared to the factor of “lethargic” sleep characteristic to initiation: the oldest mystical method of rearing. This is an assisting factor to the conscious comprehension of children in moving to a fairy tale realm, thus, enhancing the positive process undergoing in their subconscious part. The folk fairy tales have a positive impact on children whereas any changes entered in the texts of the tales or telling them voluntarily invented stories is assessed as negative.
THE PAST AND THE PRESENT OF THE GEORGIAN BUSINESS-LANGUAGE
Authors: BERIDZE Mariam
Published: April 2020

The necessity of separating out business language as the object of special studies is the problem at hand considered in the represented paper. The essence of the term branch language at one hand and its relation to the notion such as functional style at the other, are analyzed here. This issue is topical as far as it has become obvious that recently, in international as well as in Georgian linguistics we meet the term language used instead of the term functional style. Our discussion is based on the correlations between such notions as the branch language, language style and diglossia. For this goal, the history of creation and setting up of the most part of the Georgian economical terminology is discussed. This terminology was fundamentally set up and determined after the Tbilisi State University opened and began its scientific activities. The research showed that the Georgian language did not meet the novelties brought by the economical development in the 20th and 21st centuries unprepared. The represented article concerns such issues as: a term as a nomen; the means to distinguish between nomenclature and usual appellative (especially in the phraseology units and syntagmatic pairs) and principles for their study.
Analysis of the corresponding material confirms that the principles of so called grammatical organization of the terminology are common; these principles repeat the basic tendencies of “adjusting” the main tendencies of the terminology as a branch of science. The methods of observation, description, analyzing, comparing and correlating are used in the process of investigation.
General preparation of liposomes using probe-tip sonication v1
Authors: Rieth Monica , Lozano Andrew , Grant Jordan
Published: April 2020

This method outlines a general approach for preparing liposomes using probe-tip sonication. The method has been optimized for the preparation of pure DPPC liposomes on a 25-mg scale and may require modifications as the quantity of lipid is altered or upon addition of other lipids and small molecules.
An error-corrected panel-based next-generation sequencing assay for ultra-sensitive somatic mutation detection in ctDNA v1
Authors: Fettke Heidi
Published: April 2020

Circulating tumour DNA (ctDNA), fragmented DNA shed into the circulation from tumour cells, holds the potential to become a minimally-invasive biopsy material with which to interrogate the tumour landscape. However, ctDNA can be highly diluted in normal cell-free DNA (cfDNA) (released into the blood primarily by hematopoietic cells), and thus robust liquid biopsy assays with high analytical sensitivity are required for ctDNA to reach its full clinical potential in molecular genotyping. This protocol uses optimised off-the-shelf reagents in order to generate indexed and molecularly-tagged cfDNA and germline DNA libraries compatible with Illumina short-read sequencing. This protocol involves the hybridisation-capture of targeted regions of the genome using RNA baits - and has been used previously with a 42-gene panel 447 kbp in size. Also described here is the bioinformatic tools used for data processing and variant identification. The combination of deep-sequencing and error-correction based on molecular barcoding allows high sensitivity and specificity to be generated at low variant frequencies.
Tissue Preparation for Spatial Metabolomics v1
Authors: Zhang Guanshi , Velickovic Dusan , Pamreddy Annapurna , Lukowski Jessica , Alexandrov Theodore , Anderton Chris , Sharma Kumar
Published: April 2020

Mass spectrometry imaging (MSI) is a cutting-edge molecular technology that enables simultaneous analysis of multiple molecular components directly from single cells, tissues, and organs. For MSI, cryosections are prepared from flash-frozen tissue and mounted on a contuctive glass slide. We use matrix-assisted laser desorption ionization (MALDI)-MSI, where tissue sections are coated with a MALDI matrix in order to facilitate laser ionization and detection of metabolites with mass spectrometry. We do it using an automated robotic sprayer (TM-Sprayer) with 2,5- dihydroxybenzoic acid (DHB) MALDI matrix for positive ion mode analysis, N-(1-naphthyl) ethylenediamine hydrochloride (NEDC) for negative ion mode analyses, or 1,5-Diaminonaphthalene (DAN) for dual-polarity analysis, prior to being loaded into the MALDI-Q Exactive HF-X Orbitrap-MSI or MALDI-FTICR-MSI for untargeted metabolomics analysis. We have optimized a method for matrix application to maximize analyte extraction from tissue and increase MALDI sensitivity and to create the most homogenous matrix films possible for best lateral resolution of lipids. A key capacity that will be critical for scale-up in KPMP is the use of a TM-sprayer robotic sprayer for MALDI matrix application. This will make inter-lab studies viable, by increasing the reproducibility of sample preparation and is currently in use at UTHSA and PNNL. Our lipid extraction protocol for LC-MS/MS is a robust and universal protocol for single-sample integrative proteomics, metabolomics, and lipidomics analyses (see citation).
DAB Detection of Biocytin Labeled Tissue v4
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol describes the process for diaminobenzidine (DAB) detection of biocytin filled cells. This protocol is optimized for use with brain slices cut at 350 µm thick, in which cells are first filled with biocytin (i.e., post-electrophysiological recording), fixed in 4% PFA/2.5% glutaraldehyde, and transferred to PBS until ready to stain. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Slice Preparation and Blockface Imaging for Electrophysiology - Mouse v4
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol describes the procedure for transcardial perfusion of an adult mouse, followed by dissection, embedding, slicing, and blockface imaging of the mouse brain using either a Leica vibratome or a compresstome. The output tissue is suitable for use in electrophysiological recordings and/or live cell filling applications. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Post Patch Clamp Slice Fixation v4
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol describes the process to fix recorded/filled cells from mouse and human brain slices with 4% PFA/2.5% Glutaraldehyde in PBS for future histochemical processing. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
No Sew Face Mask in 10 Steps v1
Authors: Borden Julia
Published: April 2020

This protocol describes how to make a face mask out of a t-shirt in 10 easy steps, no sewing required!
Sars-CoV2 RNA purification with homemade SPRI beads for RT-qPCR test v1
Authors: Rahat Ayelet , Adam Miriam , Shabi Uri , Cohen Moshe , Kitsberg Daniel , Nissim-Rafinia Malka , Turm Hagit , Klochendler Agnes , Joseph-Strauss Daphna , Sharkia Israa , Lotem Matan , Fialkoff Gavriel , Sadeh Ronen , Chappleboim Alon , Dor Yuval , Friedman Nir , Wolf Dana , Habib Naomi
Published: April 2020

The current SARS-CoV2 epidemic calls for large scale viral tests. The current testing procedure for the presence of virions involves several steps - pharyngeal/nasal swab, cell/viral lysis, RNA extraction, and an rtPCR assay targeting the viral genome and a human gene as an internal sample control. Improvement of any of these steps in terms of cost, processing time, or reagent availability, could result in a significant increase of testing capacity world-wide. Here, we describe a rapid and efficient home made SPRI-based RNA extraction method from a lysed sample. Our approach can be fully automated, is quick (<30 min for 96 samples) and cheap (<1$ per sample), and was tested successfully on more than 250 clinical samples with approved rt-qPCR detection kits.
Human Post Mortem Brain Processing v1
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol describes the steps for processing post-mortem human brain, starting from whole brain or a single hemisphere. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U01MH114812. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health
Fresh 4% Paraformaldehyde in PBS v1
Authors: Institute for Brain Science Allen
Published: April 2020

Freshly prepared 4% Paraformaldehyde in PBS is used to fix adult and developing mouse brains during the transcardial perfusion process, as well as for immersion fixation. This solution can be used for no more than one week post-preparation date. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Artificial Cerebrospinal Fluid I (ACSF.I) v1
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol is used to prepare Artificial Cerebrospinal Fluid I (ACSF.I). ACSF.I is used for applications including transcardial perfusion prior to fresh mouse brain slicing and as a bathing solution during fresh mouse brain slicing. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Knowledge, attitudes and quality of life of caregivers towards asthma in their children: a Nigerian perspective
Authors: Kosisochi Chinwendu Amorha  , Ayogu Ebere Emilia , Ngwoke Blessing Adaora , Okonta Eleje Oboma
Published: April 2020

Introduction: The burden of uncontrolled asthma is high and caregivers can offer support in the management of asthma. Asthma is one of the most common chronic diseases in children. The objective of this study was to assess the knowledge, attitudes, and quality of life (QoL) of caregivers toward asthma in their children.

Methods: This cross-sectional study was conducted in the Paediatric Respiratory Unit of the University of Nigeria Teaching Hospital, Ituku-Ozalla, Enugu State (July 2017-September 2017). We utilized a 46-item questionnaire comprising knowledge and attitude domains and the 13-item Pediatric Asthma Caregiver’s QoL Questionnaire (PACQLQ). Data were analyzed using the IBM SPSS Version 25.0. Statistical significance was set at p < 0.05.

Results: Fifty-one caregivers participated in the study. More than half (n = 36, 70.6%) of the caregivers were 40 years old and above, female (n = 37, 72.5%), graduates from higher institutions (n = 33, 64.7%), and self-employed (n = 27, 52.9%). About a quarter (n = 13, 25.5%) had a family history of asthma and a similar proportion (n = 14, 27.5%) knew the three main symptoms of asthma.

Conclusion: Less than half (n = 24, 47.1%) of the caregivers had good asthma knowledge. Dust (n = 35, 68.6%) and smoke (n = 31, 60.8%) were identified as the most common asthma triggers in their children. The majority of the caregivers (n = 41, 80.3%) agreed that most people can have well-controlled asthma without seeing a doctor regularly. Overall, less than half of the caregivers (n = 24, 47.1%) showed positive attitudes toward their children’s asthma. The overall score for the PACQLQ was 3.91 (0.98) which implied a poor QoL. The caregivers had both impaired activity and emotional function from managing asthma in their children. More female caregivers had better knowledge about asthma than their male counterparts (t = −3.178; df = 49; p = 0.003). Less than half of the caregivers had good asthma knowledge and positive attitudes toward asthma in their children. They had an impaired QoL from managing asthma in their children.
2006 Filistin Seçimi Sonrasında Dışlama ve Dâhil Etme Ekseninde HAMAS’ın Uluslararası Toplumla İlişkisi (2006-2010)
Authors: Katman Filiz
Published: April 2020

Bu çalışmada, sistem düzeyinde 2006 Filistin seçiminden sonraki ilk dört yıllık iktidar döneminde (2006-2010) HAMAS’ın seçim zaferinin uluslararası toplumun HAMAS’a bakışının HAMAS’a ne yönde etkisinin olduğu incelenmektedir. Bu bağlamda HAMAS’a bakışta dışlama ve dâhil etme yaklaşımı ele alınmıştır. HAMAS’ın da 2006 seçimlerinden sonraki ilk dört yılda küresel izolasyondan çıkmanın yollarını araştırmakta olduğu görülmektedir. Dışlama ve dâhil etme yaklaşımlarında süreci etkileyen faktörler ve öğrenen örgüt niteliği göz önüne alındığında HAMAS’ın yaşadıklarından edindiği deneyimler ve esnekliği sayesinde, oluşan duruma uygun şekilde pozisyon alıp söylem ve eylemini bu doğrultuda geliştirdiğinden yola çıkarak, dışlamanın radikalizmi körüklerken dâhil etmenin HAMAS’ın söylemlerinde siyasî ifadelerin şiddet ifadelerine oranla daha fazla yer alması ve rakibiyle koalisyon kurma girişimlerinde bulunmasına yola açtığı; dâhil etme noktasında belirleyici faktörün ise HAMAS’ın şiddeti tamamen bırakması olduğu görülmektedir.
Implantação do Planejamento Tributário em Empresas Prestadoras de Serviço
Authors: Arakaki João Felipe Leguizamon , Assunção Ayron Vinícius Pinheiro de , Lopes Michelle Da Rosa , Zanão Eduardo Henrique
Published: April 2020

O presente artigo trata da implantação do Planejamento Tributário para empresa de prestação de serviço, como aplicação das ferramentas de software Open Project e WBS Chart Pro. As razões que embasaram este estudo estão vinculadas com as dificuldades enxergadas pelo empresariado para tratar as questões tributárias, visto a complexidade das leis tributárias que vigoram no país, o que acaba levando inúmeros empresários a praticarem atos ilegais, caracterizados como evasão fiscal. Os resultados obtidos possibilitaram, além da implantação do novo produto, uma nova organização empresarial, levando ao corpo técnico uma nova visão do que vem a ser o gerenciamento de projetos e quais as suas infinitas possibilidades dentro do mundo corporativo. Palavras-chave: Planejamento Tributário. Open Project. Gerenciamento de Projetos. AbstractThis paper deals with the implementation of  Tax Planning for a service company, as an application of the Open Project and WBS Chart Pro software tools. The reasons for this study are related to the difficulties faced by the business community in dealing with tax issues, the complexity of the tax laws in force in the country, which leads many businessmen to engage in illegal acts, characterized as tax evasion. The results obtained enabled, in addition to the implementation of the new product, a new business organization, leading to the technical staff a new vision of what is project management and what its infinite possibilities within the corporate world are. Keywords: Tax Planning; Open Project; Project management.
Two Speeds: Time of Three Opera and Time of Users
Authors: Leto Francesca
Published: April 2020

The form of faith is a living form, starting from a Urform, according to an aesthetics manner. Art and architecture have the same virtual capacity of ritual to create the counterintuitive world. We have two ways of transmitting faith: repetition and emotion. Repetition is the form of the ritual and emotion is what all the three opera must have in common. Liturgy, Art and Architecture are understood as opus. There is a temporal relationship between vital becoming of this three opera and the user. Forms change over time and they should continue communicating on the basis of a positive relationship with users. In the Italian context there is a fracture between some users and the making of the form. The fracture needs to be reconciled through an appropriate education of principals and worshippers. The methodological process for Alberto Gianfreda’s liturgical adaptation of Tolentini’s church in Venice is brought to attention.
Requirements, Qualities and Solutions
Authors: Urbán Erzsébet
Published: April 2020

After the Second World War the ecclesiastical property was drastically reduced in Hungary. However, Vatican II gave an intense motivation and impulse to the spiritual-psychical revival for the communities and by this time, the preservation of the religious heritage got some statal, professional attention too. The restoration or reconstruction works were often linked with the acute interior transformations according to Vatican II. Although the Hungarian publication and interpretation of the reforms were relatively slow, still a few essential informative discussions were born. The Venice Charter (1964) also had a significant effect on the monument preservation methodology in this period. Analysing the theoretical approach of the Vatican II Constitution and Instruction a close parallelism can be identified with the architectural aspects of the Venice Charter: respect the old parts with the obviously distinguishable, new supplements, and create modern artworks with high artistic quality. The end of the paper cites some brief case studies to present the practical implementation of the directives.
The Global Reach of Social Work:
Authors: Richards-Schuster Katie , Ruffolo Mary , Hoffman Charity , Kwesele Change
Published: April 2020

Technological innovations in social work education support efforts to expand the global reach of social work education. Massive open online courses (MOOCs) represent one type of innovation; however, little is known about their potential for promoting global social work. In 2015, our school of social work developed the first known social work MOOC using the edX platform. In this paper, we explore the initial voluntary survey data from registrants (n=992) to determine participation patterns and motivations. Our data indicates that the MOOC engaged participants from over 180 countries, with over one-third (35%) from the United States. Over 40% of participants rated themselves as novices with social work content. Thematic analysis of open-ended responses suggests that many participants were working in organizations or NGOs and wanted information about how to be more effective as a worker in these settings. Their participation in the MOOC allowed for exploration of a social work educational program. Further analysis of demographic trends, motivations, and participation levels suggests that MOOCs can be a platform for extending the reach of social work education to global social work settings. More research is needed to fully explore the potential of this educational platform for expanding the reach of social work education globally.
Dual Master of Social Work – Master of Public Health (MSW/MPH)
Authors: Reeves Patricia M. , Salm Ward Trina Colleen
Published: April 2020

While there has been a proliferation of MSW/MPH programs concurrent with dramatic changes in the U.S. health system, there is minimal research on these programs. The purpose of this article is to describe the conceptualization, development, and implementation of an innovative MSW/MPH program at a southeastern university—the only such program in the state. Our goal as the first two directors of the program, serving consecutively, is to share knowledge and offer “lessons learned” for universities seeking to develop or enhance an MSW/MPH program, as well as agencies interested in forming collaborative partnerships. “Lessons learned” include the importance of strong ongoing communication among all MSW/MPH stakeholders, thoughtful consideration of the time demands associated with the program director’s role, viewing a developmental evaluation plan as a critical component for success, and recognizing the benefits of purposeful linkages between the two disciplines.
Explorando o Vault 11: espaço narrativo em Fallout – New Vegas
Authors: Abrão José
Published: April 2020

Este trabalho visa apresentar, de modo didático, qual o diferencial dos video games como forma de comunicação, especialmente narrativa. Para isso propomos uma relação entre espaço e vídeo games. Usamos como ponto de partida a agência do jogador e sua imersão como forma de construir significado e de inserir o jogador no mundo digital narrativamente. Para isto usamos como estudo de caso a forma como o Vault 11, do jogo Fallout: New Vegas é apresentado e como o jogador interage com o mesmo.
A representação social dos partidos políticos no Brasil e Portugal: a opinião dos internautas sobre os partidos de seus países
Authors: Queiroz Marcos Marinho
Published: April 2020

Os partidos políticos são instituições importantes para a manutenção de sistemas democráticos como o brasileiro e o português. Perceber o alinhamento entre a comunicação destes partidos e a significação que deles fazem os internautas dos dois países pode servir de indício sobre a possível necessidade de as siglas partidárias repensarem as formas e conteúdos daquilo que têm disseminado, bem como questionarem como estão a ser representados pela mídia para receberem por parte da população uma significação completamente negativa. Esta pesquisa apresenta a representação social dos partidos políticos brasileiros e portugueses na perspectiva de internautas que, em sua maioria, os consideram corruptos.