bluuebunny/crossref_metadata_2025 huggingface dataset:
METTL16 regulates m6A methylation on chronic hepatitis B associated gene HLA-DPB1 involved in liver fibrosis
Authors: Gao Haibing , Wang Xiangmei , Ma Huaxi , Lin Shenglong , Zhang Dongqing , Wu Wenjun , Liao Ziyuan , Chen Mengyun , Li Qin , Lin Minghua , Li Dongliang
Published: November 2022

The role of genetic factors in the occurrence and progression of CHB (CHB) is still not fully explored. In recent years, genome-wide association studies on CHB patients have demonstrated that a large number of CHB-associated single nucleotide polymorphisms exist in the gene intron, which may regulate expression at the transcriptional level. Modification of RNA m6A methylation is one of the key mechanisms regulating gene expression. Here we show that METTL16, an m6A regulator involved in mRNA intron splicing, is differentially expressed in CHB the tissue of patients who has definite diagnosis of mild and severe fibrosis. At the same time, there are also significant differences in the expression of CHB-associated genes such as HLA-DPA1 and HLA-DPB1. The expression of HLA-DPB1 is related to METTL16. Furthermore, analyses of RNA binding of METTL16 and HLA-DPB1 show that the silencing of METTL16 in astrocytes downregulates m6A and expression of HLA-DPB1. In conclusion, METTL16 participates in the progression of CHB fibrosis by regulating the m6A level and expression of HLA-DPB1.
Politico-ideological violence in Lebanon: The narrative embeddedness of grievances
Authors: Ajil Ahmed
Published: November 2022

This paper presents the findings from doctoral research conducted between 2018 and 2020 on politico-ideological mobilization and violence in relation to causes and conflicts in the Arab World. It focuses on interviews conducted in Lebanon with individuals engaged in violent action or sympathizing with violent groups. Ideologically, the sample comprised a variety of orientations, including Christian right-wing, Salafi-jihadist and Shia militantism. The socio-economic, ethno-racial and political grievances expressed by interviewees are analyzed in-depth and the importance of collective memories, identities and narratives is elaborated on. It is argued that grievances, in order to be sustained over time and space, need to decomplexify reality by allowing for analysis to escape to the global, the collective and the past. Grievances are narratively embedded in a framework that simplifies reality in order to pinpoint injustices and suggest straightforward actions for remedying them.
Identifying hub genes of calcific aortic valve disease and revealing the immune infiltration landscape based on multiple WGCNA and single-cell sequence analysis
Authors: Wang Kan , Zheng Qiang , Liu Xing , Geng BingChuan , Dong NianGuo , Shi JiaWei
Published: November 2022

Background

Calcific aortic valve disease (CAVD) is a progressive fibrocalcific disease that can be treated only through valve replacement. This study aimed to determine the role of hub genes and immune cell infiltration in CAVD progression.

Methods

In this study, bioinformatics analysis was used to identify hub genes involved in CAVD. The datasets were downloaded from the Gene Expression Omnibus (GEO) database. Gene expression differences were evaluated via pathway and Gene Ontology analyses. Weighted gene co-expression network analysis (WGCNA) and differentially expressed genes were used to screen hub genes. The CIBERSORT algorithm was used to compare immune infiltration into the calcified aortic valve based on the hub genes between high- and low-expression groups. We also performed single-cell RNA sequencing based on six different human aortic valve leaflets. The expression of hub genes was identified in human and mouse samples through quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry, immunofluorescence, and ELISA, and clinical features of the patients were investigated.

Results

In total, 454 differentially expressed genes were obtained from the GEO database. WGCNA was used to find 12 co-expression modules in the Array Express database, of which one hub module (brown module) was most correlated with CAVD. Two hub genes were identified after combining the differentially expressed genes S100A8 and S100A9. Regarding these genes, the immune infiltration profiles varied between high- and low-expression groups. Compared with that in the low hub gene expression group, the high hub gene expression group had a higher proportion of activated NK cells (p < 0.01) and M1 macrophages (p < 0.05). The expression of S100A8 and S100A9 was consistent with single-gene RNA sequencing results, confirming that the expression levels of these two hub genes are significantly upregulated in patients with CAVD (p < 0.01). Furthermore, these results were verified using mouse and human samples by performing immunofluorescence, immunohistochemistry, qPCR, and ELISA analyses. Finally, the localization of S100A8 and S100A9 in monocytes and macrophages was confirmed via immunofluorescence using human aortic valves.

Conclusion

These results demonstrate that S100A8 and S100A9 are two hub genes involved in CAVD, which might play an important role in its development through immune-related signaling pathways.
Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach
Authors: Nandi Abhishek , Yadav Ruchi , Singh Aditi
Published: November 2022

Acinetobacter baumannii is a bacterial pathogen that is commonly associated with hospital-acquired illnesses. Antimicrobial drug resistance in A. baumannii includes several penicillin classes, first and second-generation cephalosporins, cephamycins, most aminoglycosides, chloramphenicol, and tetracyclines. The recent rise in multidrug-resistant A. baumannii strains has resulted in an increase in pneumoniae associated with ventilators, urinary tract infections associated with the catheter, and bloodstream infections, all of which have increased complications in treatment, cost of treatment, and death. Small compounds known as antimicrobial peptides (AMPs) are known to have damaging effects on pathogenic bacteria. To determine their antimicrobial activity, AMPs are created from proteins acquired from various sources and evaluated in vitro. In the last phase of lytic cycle, bacteriophages release hydrolytic enzymes called endolysins that cleave the host’s cell wall. Due to their superior potency and specificity compared to antibiotics, lysins are used as antibacterial agents. In the present study, different types of endolysin from phages of A. baumannii were selected based on an extensive literature survey. From the PhaLP database, the sequences of the selected lysins were retrieved in FASTA format and antimicrobial peptides were found among them. With the help of available bioinformatic tools, the anti-biofilm property, anti-fungal property, cell-penetrating property, and cellular toxicity of the antimicrobial peptides were determined. Out of the fourteen antimicrobial peptides found from the eight selected endolysins of A. baumannii specific phage, eight of them has anti-biofilm property, nine of them has anti-fungal property, five of them has cell-penetrating property and all of them are non-toxic.
Analysis of pathogenesis and drug treatment of chronic obstructive pulmonary disease complicated with cardiovascular disease
Authors: Li Xiao-Fang , Wan Cheng-Quan , Mao Yi-Min
Published: November 2022

Chronic obstructive pulmonary disease (COPD) is a disease characterized by persistent airflow limitation, and is associated with abnormal inflammatory responses in the lungs to cigarette smoke and toxic and harmful gases. Due to the existence of common risk factors, COPD is prone to multiple complications, among which cardiovascular disease (CVD) is the most common. It is currently established that cardiovascular comorbidities increase the risk of exacerbations and mortality from COPD. COPD is also an independent risk factor for CVD, and its specific mechanism is still unclear, which may be related to chronic systemic inflammation, oxidative stress, and vascular dysfunction. There is evidence that chronic inflammation of the airways can lead to destruction of the lung parenchyma and decreased lung function. Inflammatory cells in the airways also generate reactive oxygen species in the lungs, and reactive oxygen species further promote lung inflammation through signal transduction and other pathways. Inflammatory mediators circulate from the lungs to the whole body, causing intravascular dysfunction, promoting the formation and rupture of atherosclerotic plaques, and ultimately leading to the occurrence and development of CVD. This article reviews the pathophysiological mechanisms of COPD complicated by CVD and the effects of common cardiovascular drugs on COPD.
Identification and analysis of the interaction network of African swine fever virus D1133L with host proteins
Authors: Hao Yu , Yang Jinke , Yang Bo , Zhang Ting , Shi Xijuan , Yang Xing , Zhang Dajun , Zhao Dengshuai , Yan Wenqian , Chen Lingling , Liu Xiangtao , Zheng Haixue , Zhang Keshan
Published: November 2022

African swine fever (ASF) is a contagious and lethal hemorrhagic disease in pigs; its spread results in huge economic losses to the global pig industry. ASF virus (ASFV) is a large double-stranded DNA virus encoding >150 open reading frames. Among them, ASFV-encoded D1133L was predicted to be a helicase but its specific function remains unknown. Since virus-host protein interactions are key to understanding viral protein function, we used co-immunoprecipitation combined with liquid chromatography-mass spectrometry to investigate D1133L. This study describes the interaction network of ASFV D1133L protein in porcine kidney PK-15 cells. Overall, 1,471 host proteins that potentially interact with D1133L are identified. Based on these host proteins, a protein–protein network was constructed. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that cellular D1133L-interacted proteins are involved in the ribosome, spliceosome, RNA transport, oxidative phosphorylation, proteasome, and DNA replication. Vimentin (VIM), tripartite motif-containing protein 21 (TRIM21), and Tu translation elongation factor (TUFM) were confirmed to interact with D1133L in vitro. VIM or TRIM21 overexpression significantly promoted ASFV replication, but TUFM overexpression significantly inhibited ASFV replication. These results help elucidate the specific functions of D1133L and the potential mechanisms underlying ASFV replication.
Resistance to aztreonam-avibactam due to CTX-M-15 in the presence of penicillin-binding protein 3 with extra amino acids in Escherichia coli
Authors: Ma Ke , Zong Zhiyong
Published: November 2022

Aztreonam-avibactam is a promising combination to treat carbapenem-resistant Enterobacterales including coverage for metallo-β-lactamases. Escherichia coli strains resistant to aztreonam-avibactam have emerged but resistance mechanisms remain to be elucidated. We performed a study to investigate the mechanism for aztreonam-avibactam in a carbapenem-resistant Escherichia coli clinical strain. This strain was resistant to aztreonam-avibactam (aztreonam MIC, 16 mg/L in the presence of 4 mg/L avibactam). Whole genome sequencing revealed that the strain carried metallo-β-lactamase gene blaNDM-4 and the extended-spectrum β-lactamase (ESBL) gene blaCTX-M-15 and had a YRIK four amino acid insertion in penicillin-binding protein 3 (PBP3). blaCTX-M-15 was cloned into pET-28a(+), followed by the transformation, with the gene, of E. coli strain 035125∆pCMY42 possessing the YRIK insertion in PBP3 and strain BL21 with the wildtype PBP3. blaCTX-M-14, another common ESBL gene, and blaCTX-M-199, a hybrid of blaCTX-M-14 and blaCTX-M-15 were also individually cloned into both E. coli strains for comparison. Aztreonam-avibactam resistance was only observed in the E. coli strains with the YRIK insertion in PBP3 that produced CTX-M-15 or its hybrid enzyme CTX-M-199. Checkerboard titration assays were performed to determine the synergistic effects between aztreonam-avibactam and ceftazidime or meropenem. Doubling avibactam concentration in vitro reversed aztreonam-avibactam resistance, while the combination of aztreonam-avibactam and ceftazidime or meropenem did not. In conclusion, CTX-M enzymes with activity against aztreonam, (e.g., CTX-M-15 and CTX-M-199), can confer resistance in the combination of PBP3 with YRIK insertions in metallo-β-lactamase-producing carbapenem-resistant E. coli. Doubling the concentration of avibactam may overcome such resistance.
Microbial regulation of offspring diseases mediated by maternal-associated microbial metabolites
Authors: Jiang Qingru , Li Tian , Chen Wei , Huo Yingfang , Mou Xiangyu , Zhao Wenjing
Published: November 2022

The microbiota plays a crucial role in individuals’ early and long-term health. Previous studies indicated that the microbial regulation of health may start before birth. As the in utero environment is (nearly) sterile, the regulation is probably be originated from maternal microbiota and mediated by their metabolites transferred across the placenta. After the birth, various metabolites are continuously delivered to offspring through human milk feeding. Meanwhile, some components, for example, human milk oligosaccharides, in human milk can only be fermented by microbes, which brings beneficial effects on offspring health. Hence, we speculated that human milk-derived metabolites may also play roles in microbial regulation. However, reports between maternal-associated microbial metabolites and offspring diseases are still lacking and sparsely distributed in several fields. Also, the definition of the maternal-associated microbial metabolite is still unclear. Thus, it would be beneficial to comb through the current knowledge of these metabolites related to diseases for assisting our goals of early prediction, early diagnosis, early prevention, or early treatment through actions only on mothers. Therefore, this review aims to present studies showing how researchers came to the path of investigating these metabolites and then to present studies linking them to the development of offspring asthma, type 1 diabetes mellitus, food allergy, neonatal necrotizing enterocolitis, or autism spectrum disorder. Potential English articles were collected from PubMed by searching terms of disease(s), maternal, and a list of microbial metabolites. Articles published within 5 years were preferred.
Antibiotic-resistant Campylobacter coli and Campylobacter jejuni in commercial and smallholder farm animals in the Asante Akim North Municipality of Ghana
Authors: Paintsil Ellis Kobina , Ofori Linda Aurelia , Akenten Charity Wiafe , Zautner Andreas E. , Mbwana Joyce , Jaeger Anna , Lamshöft Maike , May Jürgen , Obiri-Danso Kwasi , Philipps Richard Odame , Krumkamp Ralf , Dekker Denise
Published: November 2022

Worldwide, farm animals, in particular poultry, are an important reservoir for Campylobacter spp. However, information on Campylobacter colonization in farm animals in Africa is scarce. Hence, this cross-sectional study determined antibiotic-resistant Campylobacter from both commercial and smallholder farm animals in the Asante Akim North Municipality of Ghana. Fecal samples from poultry and livestock kept by commercial and smallholder farms were collected and analyzed using standard microbiological methods. The overall Campylobacter frequency was 20.3% (n/N = 322/1,585), and frequencies detected were similarly high in isolates from commercial (21.0%, n/N = 169/805) and smallholder (19.6%, n/N = 153/780) farms. Species isolated were C. coli (67.7%, n/N = 218/322) and C. jejuni (32.3%, n/N = 104/322). However, the frequency of C. coli was 2.1 (95% CI: 1.8–2.5) times higher than what was found for C. jejuni. Campylobacter frequencies in the rainy season was 22.2% (n/N = 258/1,160) and 15.1% (n/N = 64/425) in the dry season (prevalence ratio = 1.48, 95% CI: 1.2–1.9). About 1.7% (n/N = 6/322) of the Campylobacter isolates, all from smallholder farms, were susceptible to all antibiotics tested. Multidrug resistance was observed for 4.7% (n/N = 15/322) of the Campylobacter isolates, of which 93.3% (n/N = 14/15) occurred in isolates from commercial farms. This study highlights the need for the implementation of control programs, in commercial farming but also at the smallholder farm level, to formulate clear guidelines aimed at decreasing Campylobacter contamination of meat products and reducing the use of antibiotics in the farming sector.
Transient Ca2+ entry by plasmalogen-mediated activation of receptor potential cation channel promotes AMPK activity
Authors: Honsho Masanori , Mawatari Shiro , Fujino Takehiko
Published: November 2022

Ethanolamine-containing alkenyl ether glycerophospholipids, plasmalogens, are major cell membrane components of mammalian cells that activate membrane protein receptors such as ion transporters and G-protein coupled receptors. However, the mechanism by which plasmalogens modulate receptor function is unknown. Here, we found that exogenously added plasmalogens activate transient receptor potential cation channel subfamily C member 4 (TRPC4) to increase Ca2+ influx, followed by calcium/calmodulin-dependent protein kinase 2-mediated phosphorylation of AMP-activated protein kinase (AMPK). Upon topical application of plasmalogens to the skin of mice, AMPK activation was observed in TRPC4-expressing hair bulbs and hair follicles. Here, TRPC4 was co-localized with the leucine-rich repeat containing G protein-coupled receptor 5, a marker of hair-follicle stem cells, leading to hair growth. Collectively, this study indicates that plasmalogens could function as gate openers for TRPC4, followed by activating AMPK, which likely accelerates hair growth in mice.
Prophylactic action of ayahuasca in a non-human primate model of depressive-like behavior
Authors: de Meiroz Grilo Maria Lara Porpino , Sousa Geovan Menezes de , Mendonça Lilían Andrade Carlos de , Lobão-Soares Bruno , Sousa Maria Bernardete Cordeiro de , Palhano-Fontes Fernanda , Araujo Draulio Barros de , Perkins Daniel , Hallak Jaime Eduardo Cecilio , Galvão-Coelho Nicole Leite
Published: November 2022

Observational studies of long-term users of ayahuasca, an Amazonian psychedelic brew, suggest an increase in resilience via improvements in emotion and cognition. Ayahuasca has also demonstrated clinical antidepressant effects in human and animal studies; however, its potential prophylactic action in depression has not been previously studied. Therefore, this experimental study sought to evaluate the potential prophylactic effects of repeated and long-term ayahuasca use, via the modulation of resilience, in a non-human primate animal model, Callithrix jacchus, subjected to a protocol for induction of depressive-like behavior. For the formation of the study groups, some juvenile marmosets were kept in their family groups (GF = 7), while for the two experimental groups, the animals were removed from the family and kept socially isolated. Then, part of the isolated animals made up the group in which ayahuasca was administered (AG, n = 6), while for others, no intervention was made (IG, n = 5). AG animals took ayahuasca (1.67 mL/300g body weight) at weeks 4 (before isolation), 8, and 12 (during isolation) of the study. More adaptive stress response was observed for the AG when compared to the IG. The AG showed higher cortisol reactivity and fecal cortisol levels than IG, while both measures were similar to FG. Moreover, AG animals showed no signs of anhedonia and no increase in chronic stress-related behaviors, which were expressed by the IG. Thus, ayahuasca seems to promote the expression of resilient responses, indicating a prophylactic action, buffering the emergence of depressive-like behaviors and cortisol alterations associated with major depression. These results are encouraging for further research on the prophylactic use of psychedelics to prevent psychopathologies associated with chronic stress.
Synaptic vesicle release during ribbon synapse formation of cone photoreceptors
Authors: Davison Adam , Gierke Kaspar , Brandstätter Johann Helmut , Babai Norbert
Published: November 2022

Mammalian cone photoreceptors enable through their sophisticated synapse the high-fidelity transfer of visual information to second-order neurons in the retina. The synapse contains a proteinaceous organelle, called the synaptic ribbon, which tethers synaptic vesicles (SVs) at the active zone (AZ) close to voltage-gated Ca2+ channels. However, the exact contribution of the synaptic ribbon to neurotransmission is not fully understood, yet. In mice, precursors to synaptic ribbons appear within photoreceptor terminals shortly after birth as free-floating spherical structures, which progressively elongate and then attach to the AZ during the following days. Here, we took advantage of the process of synaptic ribbon maturation to study their contribution to SV release. We performed whole-cell patch-clamp recordings from cone photoreceptors at three postnatal (P) development stages (P8–9, P12–13, >P30) and measured evoked SV release, SV replenishment rate, recovery from synaptic depression, domain organization of voltage-sensitive Ca2+ channels, and Ca2+-sensitivity of exocytosis. Additionally, we performed electron microscopy to determine the density of SVs at ribbon-free and ribbon-occupied AZs. Our results suggest that ribbon attachment does not organize the voltage-sensitive Ca2+ channels into nanodomains or control SV release probability. However, ribbon attachment increases SV density at the AZ, increases the pool size of readily releasable SVs available for evoked SV release, facilitates SV replenishment without changing the SV pool refilling time, and increases the Ca2+- sensitivity of glutamate release.
What microglia depletion approaches tell us about the role of microglia on synaptic function and behavior
Authors: Basilico Bernadette , Ferrucci Laura , Khan Azka , Di Angelantonio Silvia , Ragozzino Davide , Reverte Ingrid
Published: November 2022

Microglia are dynamic cells, constantly surveying their surroundings and interacting with neurons and synapses. Indeed, a wealth of knowledge has revealed a critical role of microglia in modulating synaptic transmission and plasticity in the developing brain. In the past decade, novel pharmacological and genetic strategies have allowed the acute removal of microglia, opening the possibility to explore and understand the role of microglia also in the adult brain. In this review, we summarized and discussed the contribution of microglia depletion strategies to the current understanding of the role of microglia on synaptic function, learning and memory, and behavior both in physiological and pathological conditions. We first described the available microglia depletion methods highlighting their main strengths and weaknesses. We then reviewed the impact of microglia depletion on structural and functional synaptic plasticity. Next, we focused our analysis on the effects of microglia depletion on behavior, including general locomotor activity, sensory perception, motor function, sociability, learning and memory both in healthy animals and animal models of disease. Finally, we integrated the findings from the reviewed studies and discussed the emerging roles of microglia on the maintenance of synaptic function, learning, memory strength and forgetfulness, and the implications of microglia depletion in models of brain disease.
A computational classification method of breast cancer images using the VGGNet model
Authors: Khan Abdullah , Khan Asfandyar , Ullah Muneeb , Alam Muhammad Mansoor , Bangash Javed Iqbal , Suud Mazliham Mohd
Published: November 2022

Cancer is one of the most prevalent diseases worldwide. The most prevalent condition in women when aberrant cells develop out of control is breast cancer. Breast cancer detection and classification are exceedingly difficult tasks. As a result, several computational techniques, including k-nearest neighbor (KNN), support vector machine (SVM), multilayer perceptron (MLP), decision tree (DT), and genetic algorithms, have been applied in the current computing world for the diagnosis and classification of breast cancer. However, each method has its own limitations to how accurately it can be utilized. A novel convolutional neural network (CNN) model based on the Visual Geometry Group network (VGGNet) was also suggested in this study. The 16 layers in the current VGGNet-16 model lead to overfitting on the training and test data. We, thus, propose the VGGNet-12 model for breast cancer classification. The VGGNet-16 model has the problem of overfitting the breast cancer classification dataset. Based on the overfitting issues in the existing model, this research reduced the number of different layers in the VGGNet-16 model to solve the overfitting problem in this model. Because various models of the VGGNet, such as VGGNet-13 and VGGNet-19, were developed, this study proposed a new version of the VGGNet model, that is, the VGGNet-12 model. The performance of this model is checked using the breast cancer dataset, as compared to the CNN and LeNet models. From the simulation result, it can be seen that the proposed VGGNet-12 model enhances the simulation result as compared to the model used in this study. Overall, the experimental findings indicate that the suggested VGGNet-12 model did well in classifying breast cancer in terms of several characteristics.
A comparative analysis of masking empirical mode decomposition and a neural network with feed-forward and back propagation along with masking empirical mode decomposition to improve the classification performance for a reliable brain-computer interface
Authors: Jaipriya D. , Sriharipriya K. C.
Published: November 2022

In general, extraction and classification are used in various fields like image processing, pattern recognition, signal processing, and so on. Extracting effective characteristics from raw electroencephalogram (EEG) signals is a crucial role of the brain-computer interface for motor imagery. Recently, there has been a great deal of focus on motor imagery in the EEG signals since they encode a person’s intent to do an action. Researchers have been using MI signals to assist paralyzed people and even move them on their own with certain equipment, like wheelchairs. As a result, proper decoding is an important step required for the interconnection of the brain and the computer. EEG decoding is a challenging process because of poor SNR, complexity, and other reasons. However, choosing an appropriate method to extract the features to improve the performance of motor imagery recognition is still a research hotspot. To extract the features of the EEG signal in the classification task, this paper proposes a Masking Empirical Mode Decomposition (MEMD) based Feed Forward Back Propagation Neural Network (MEMD-FFBPNN). The dataset consists of EEG signals which are first normalized using the minimax method and given as input to the MEMD to extract the features and then given to the FFBPNN to classify the tasks. The accuracy of the proposed method MEMD-FFBPNN has been measured using the confusion matrix, mean square error and which has been recorded up to 99.9%. Thus, the proposed method gives better accuracy than the other conventional methods.
Light-efficient channel attention in convolutional neural networks for tic recognition in the children with tic disorders
Authors: Geng Fudi , Ding Qiang , Wu Wanyu , Wang Xiangyang , Li Yanping , Sun Jinhua , Wang Rui
Published: November 2022

Tic is a combination of a series of static facial and limb movements over a certain period in some children. However, due to the scarcity of tic disorder (TD) datasets, the existing work on tic recognition using deep learning does not work well. It is that spatial complexity and time-domain variability directly affect the accuracy of tic recognition. How to extract effective visual information for temporal and spatial expression and classification of tic movement is the key of tic recognition. We designed the slow-fast and light-efficient channel attention network (SFLCA-Net) to identify tic action. The whole network adopted two fast and slow branch subnetworks, and light-efficient channel attention (LCA) module, which was designed to solve the problem of insufficient complementarity of spatial-temporal channel information. The SFLCA-Net is verified on our TD dataset and the experimental results demonstrate the effectiveness of our method.
Intelligent recommendation system based on decision model of archive translation tasks
Authors: Lilan Chen , Yongsheng Chen
Published: November 2022

How to recruit, test, and train the intelligent recommendation system users, and how to assign the archive translation tasks to all intelligent recommendation system users according to the intelligent matching principles are still a problem that needs to be solved. With the help of proper names and terms in China’s Imperial Maritime Customs archives, this manuscript aims to solve the problem. When the corresponding translation, domain or attributes of a proper name or term is known, it will be easier for some archive translation tasks to be completed, and the adaptive archive intelligent recommendation system will also improve the efficiency of intelligent recommendation quality of archive translation tasks. These related domains or attributes are different labels of these archives. To put it simply, multi-label classification means that the same instance can have multiple labels or be labelled into multiple categories, which is called multi-label classification. With the multi-label classification, archives can be classified into different categories, such as the trade archives, preventive archives, personnel archives, etc. The system users are divided into different professional domains by some tests, for instance, system users who are good at economic knowledge and users who have higher language skills. With these labels, the intelligent recommendation system can make the intelligent match between the archives and system users, so as to improve the efficiency and quality of intelligent archive translation tasks. In this manuscript, through multi-label classification, the intelligent recommendation system can realize the intelligent allocation of archive translation tasks to the system users. The intelligent allocation is realized through the construction of intelligent control model, and verifies that the intelligent recommendation system can improve the performance of task allocation over time without the participation of task issuers.
The role of dietary patterns and erythrocyte membrane fatty acid patterns on mild cognitive impairment
Authors: Wang Xuan , Li Tiantian , Ding Huini , Liu Yuru , Liu Xiaoqiang , Yu Kang , Xiao Rong , Xi Yuandi
Published: November 2022

Background

Dietary fatty acids have been shown to be associated with the development of cognition. However, research on the role of fatty acid intake in dietary patterns and fatty acid patterns (FAPs) in the development of cognitive function is limited. The aim of this study was to explore the correlation between dietary patterns and FAPs and to provide available evidence for preventing mild cognitive impairment (MCI) through these patterns.

Materials and methods

The 973 participants aged between 65 and 85 were recruited from 2020 to 2021 for this multicenter research in Beijing. Neuropsychological tests were used for cognitive evaluation, and data of dietary intake in the past 12 months were collected with semi-quantitative food frequency questionnaire. The erythrocyte membrane fatty acid profile was tested by chromatography and mass spectrometry lipid profiling. Factor analysis was used to derive the main dietary patterns and FAPs. Pearson’s correlation or Spearman’s correlation was used to explore the association between dietary patterns and FAPs. Binary logistic regression was applied to examine the relationship between patterns and cognitive function.

Results

Six dietary patterns and six FAPs were identified, explaining 53.4 and 80.9% of the total variance separately. After adjusting all potential confounders, T3 of the pattern 1 and FAP2 were the independent protect factors for MCI, respectively (OR 0.601, 95% CI [0.395, 0.914]; OR 0.108, 95% CI [0.019, 0.623]). Rich of SM (26:0), SM (24:1), and SM (26:1) is the characteristic of FAP2. A positive correlation was found between component scores of dietary pattern1 and FAP2 (r = 0.441, p = 0.001). People who adhered to a reasonable intake of animal flesh consumed more various long-chain fatty acids as well.

Conclusion

The erythrocyte membrane metabolites, SM (26:0), SM (24:1), and SM (26:1), might function as early biomarkers for predicting or monitoring of cognitive aging in the elderly. The dietary pattern with recommended animal flesh consumption was significantly associated with FAP characterized by very long-chain SMs. This dietary pattern affected FAP, which might achieve the ultimate goal of neuroprotection through the very long-chain SMs. A rational intake of dietary fatty acids might be an effective way on preventing MCI in the elderly.
Association between blood urea nitrogen to serum albumin ratio and in-hospital mortality of patients with sepsis in intensive care: A retrospective analysis of the fourth-generation Medical Information Mart for Intensive Care database
Authors: Cai Shaoyan , Wang Qinjia , Chen Chao , Guo Chunming , Zheng Liangjie , Yuan Min
Published: November 2022

Background

This study aimed to investigate the relationship between the blood urea nitrogen to serum albumin ratio (BAR) and in-hospital mortality in patients with sepsis.

Materials and methods

This is a retrospective cohort study. All septic patient data for the study were obtained from the intensive care unit of Beth Israel Deaconess Medical Center. Adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated using multivariable Cox regression analyses. Survival curves were plotted and subgroup analyses were stratified by relevant covariates.

Results

Among 23,901 patients, 13,464 with sepsis were included. The overall in-hospital mortality rate was 18.9% (2550/13464). After adjustment for confounding factors, patients in the highest BAR quartile had an increased risk of sepsis death than those in the lowest BAR quartile (HR: 1.42, 95% CI: 1.3–1.55), using BAR as a categorical variable. When BAR was presented as a continuous variable, the prevalence of in-hospital sepsis-related death increased by 8% (adjusted HR: 1.08, 95% CI: 1.07–1.1, P < 0.001) for each 5-unit increase in BAR, irrespective of confounders. Stratified analyses indicated age interactions (P < 0.001), and the correlation between BAR and the probability of dying due to sepsis was stable.

Conclusion

BAR was significantly associated with in-hospital mortality in intensive care patients with sepsis. A higher BAR in patients with sepsis is associated with a worse prognosis in the ICU in the USA. However, further research is required to confirm this finding.
A rare association between factor H deficiency and lupus: Case report and experimental treatment with curcumin
Authors: Lunz Macedo Ana Catarina , Santisteban Lores Lazara Elena , Albuquerque José Antonio Tavares , Duarte Nilo José Coelho , Romano Paschoalina , Ebner Persio Almeida Rezende , Rezende Vinicius Marcondes , Silva Clovis A. , Andrade Luís Eduardo Coelho , Vasconcelos Dewton Moraes , Isaac Lourdes
Published: November 2022

Factor H (FH) is one of the most important regulatory proteins of the alternative pathway of the complement system. FH deficiency is a rare condition that causes unregulated C3 consumption, leading to an increased susceptibility to infections and glomerulopathies. Our previous studies have demonstrated a FH deficient patient carrying a c.452G > A, p.R127H FH mutation which leads to a misfolded protein and its retention in the endoplasmic reticulum. In his cultured fibroblasts, FH-delayed secretion was partially rescued when treated with curcumin, and once secreted, exhibited normal regulatory function. Here, we report a childhood-onset systemic lupus erythematosus (cSLE) in this FH deficient patient and the results of experimental treatment with curcumin aiming to rescue FH secretion and regulatory activity.
Problems with enuresis management—A personal view
Authors: Nevéus Tryggve
Published: November 2022

Much has happened since the end of the era when enuresis was blamed on the parents or the children themselves. Still, there are large gaps in our knowledge and large parts of modern enuresis management guidelines are (still) not based on firm evidence. In this review I will question the following commonly made assumptions regarding enuresis evaluation and treatment:

•

It is important to subdivide enuresis according to the presence of daytime symptoms

•

Voiding charts are crucial in the primary evaluation of the enuretic child

•

All children with enuresis need to be screened for behavioral or psychiatric issues

•

Concomittant daytime incontinence needs to be successfully treated before addressing the enuresis

•

Concomittant constipation needs to be successfully treated before addressing the enuresis

•

Urotherapy is a first-line treatment against enuresis

In this review I will argue that much of what we do with these children is based more on experience and well-meant but poorly supported assumptions than on evidence. Some advice and therapies are probably ineffective whereas for other treatments we lack reliable predictors of treatment response. More research is obviously needed, but awaiting new results enuresis management could be substantially simplified.
Case Report: Bilateral symmetrical primary kaposiform hemangioendothelioma of the femur
Authors: Qiu Tong , Lan Yuru , Zhou Jiangyuan , Yang Kaiying , Gong Xue , Zhang Zixin , Chen Siyuan , Ji Yi
Published: November 2022

Kaposiform hemangioendothelioma (KHE) is a rare borderline vascular tumor that usually presents as a mass of skin or deep soft tissue. We report a unique case of an 8-year-old KHE patient with bilateral symmetrical sites involving both femurs. The laboratory, radiographic, and pathological findings of the patient were minutely described. During the 6-month follow-up, the symptoms of pain and dysfunction of this patient were relieved. This study aimed to arouse clinicians’ concern about the symmetrical sites of KHE patients.
A double-blind, randomized, placebo-controlled, single-center clinical trial of jiaotaiwan for the treatment of insomnia symptoms caused by disharmony of the heart and kidney
Authors: Xia Nengzhi , Jiang Chengrou , Zhou Yiwei , Huang Qun , Hu Lufeng , Zeng Haihuan , Luo Lin , Yuan Zhengzhong
Published: November 2022

Background: Jiaotaiwan (JTW) is a classical tranquillizing prescription in traditional Chinese medicine (TCM) for the treatment of insomnia symptoms caused by disharmony of the heart and kidney (ISDHK). This study aimed to evaluate the effectiveness and safety of JTW for treating ISDHK in a double-blind, randomized, placebo-controlled trial.

Methods: From September 2018 to February 2020, 128 participants with ISDHK were included in this single-center clinical trial. All participants were equally and randomly divided into either the JTW group (2-g JTW granules, b.i.d. for 7 days) or placebo group (2-g placebo granules, b.i.d. for 7 days). Pittsburgh Sleep Quality Index (PSQI) scores were set as the primary outcome, and polysomnography (PSG), 1H-magnetic resonance spectroscopy (1H-MRS), blood tests, and Disharmony of Heart and Kidney Scoring System (DHKSS) and clinical global impression (CGI) scores were used as secondary outcomes. Laboratory tests were used to evaluate the safety of JTW. All data were collected at baseline and posttreatment.

Results: A total of 106 participants completed this clinical trial. Symptom relief was more apparent in the JTW group than the placebo group (PSQI total score: 9.34 ± 3.578 vs. 10.98 ± 3.073, respectively; p = 0.006). However, no PSG changes were observed between the two groups (p > 0.05). Higher CGI and lower DHKSS scores were observed after JTW treatment. Serum melatonin was increased in patients with ISDHK after JTW treatment (JTW, 339.09 ± 256.894 vs. placebo, 219.59 ± 169.045; p = 0.004). There were significant posttreatment differences in metabolites in the left cerebellum between the two groups (myoinositol: JTW, 13.47 ± 2.094 vs. placebo, 12.48 ± 2.449; p = 0.021; choline: JTW, 3.96 ± 0.657 vs. placebo, 3.65 ± 0.562; p = 0.008). In terms of safety, JTW had no noticeable adverse effects relative to placebo.

Conclusion: JTW was effective and well tolerated for the treatment of ISDHK. The development of large-scale trials with longer follow-up durations is recommended to provide further evidence.

Clinical Trial Registration:clinicaltrials.gov, identifier ChiCTR1800019239
A bibliometrics and visualization analysis of cannabidiol research from 2004 to 2021
Authors: Liu Liu , Liu Jianxing , Zhao Ming , Cai Meiming , Lei Fanzhang , Zeng Xiaofeng , Zhu Bofeng
Published: November 2022

Cannabidiol, a non-psychoactive component extracted from the plant cannabis sativa, has gained growing focus in recent years since its extensive pharmacology effects have been founded. The purpose of this study intends to reveal the hot spots and frontiers of cannabidiol research using bibliometrics and data visualization methods. A total of 3,555 publications with 106,793 citations from 2004 to 2021 related to cannabidiol were retrieved in the Web of Science database, and the co-authorships, research categories, keyword burst, and reference citations in the cannabidiol field were analyzed and visualized by VOSviewer and Citespace software. Great importance has been attached to the pharmacology or pharmacy values of cannabidiol, especially in the treatment of neuropsychiatric disorders, such as epilepsy, anxiety, and schizophrenia. The mechanisms or targets of the cannabidiol have attracted the extreme interest of the researchers, a variety of receptors including cannabinoids type 1, cannabinoids type 2, 5-hydroxytriptamine1A, and G protein-coupled receptor 55 were involved in the pharmacology effects of cannabidiol. Moreover, the latest developed topic has focused on the positive effects of cannabidiol on substance use disorders. In conclusion, this study reveals the development and transformation of knowledge structures and research hotspots in the cannabidiol field from a bibliometrics perspective, exploring the possible directions of future research.
A corpus-based study on Chinese and American students' rhetorical moves and stance features in dissertation abstracts
Authors: Liu Yingliang , Hu Xuechen , Liu Jiaying
Published: November 2022

Dissertation is the most important research genre for graduate students as they step into the academic community. The abstract found at the beginning of the dissertation is an essential part of the dissertation, serving to “sell” the study and impress the readers. Learning to compose a well-organized abstract to promote one's research is therefore an important skill for novice writers when they step into the academic community in their discipline. By comparing 112 dissertation abstracts in material science by Chinese and American doctoral students, this study attempts to analyze not only the rhetorical moves of dissertation abstracts but also the lexical-grammatical features of stance in different abstract moves. The findings show that most of the abstracts include five moves, namely, Situating the research, Presenting the research, Describing the methodology, Summarizing the findings, and Discussing the research. However, fewer abstracts by Chinese students include all five moves. In addition, the choices of stance expressions by the two groups vary across the five abstract moves for different communication purposes. The results of this study have pedagogical implications for facilitating the development of academic writing skills for L2 writers.
Topic modeling and sentiment analysis of Chinese people’s attitudes toward volunteerism amid the COVID-19 pandemic
Authors: Yin Ruheng , Wu Jing , Tian Rui , Gan Feng
Published: November 2022

The COVID-19 pandemic has created an urgent need for volunteers to complement overwhelmed public health systems. This study aims to explore Chinese people’s attitudes toward volunteerism amid the COVID-19 pandemic. To this end, we identify the latent topics in volunteerism-related microblogs on Weibo, the Chinese equivalent of Twitter using the topic modeling analysis via Latent Dirichlet Allocation (LDA). To further investigate the public sentiment toward the topics generated by LDA, we also conducted sentiment analysis on the sample posts using the open-source natural language processing (NLP) technique from Baidu. Through an in-depth analysis of 91,933 Weibo posts, this study captures 10 topics that are, in turn, distributed into five factors associated with volunteerism in China as motive fulfillment (n = 31,661, 34.44%), fear of COVID-19 (n = 22,597, 24.58%), individual characteristic (n = 17,688, 19.24%), government support (n = 15,482, 16.84%), and community effect (n = 4,505, 4.90%). The results show that motive fulfillment, government support, and community effect are the factors that could enhance positive attitudes toward volunteerism since the topics related to these factors report high proportions of positive emotion. Fear of COVID-19 and individual characteristic are the factors inducing negative sentiment toward volunteerism as the topics related to these factors show relatively high proportions of negative emotion. The provision of tailored strategies based on the factors could potentially enhance Chinese people’s willingness to participate in volunteer activities during the COVID-19 pandemic.
Acne caused by ziprasidone in a young patient with bipolar disorder: A case report
Authors: Yuan Yiwen , Li Xiaoqing , Jiang Xingmei , Li Zhixiong , Ou Ying , Li Zhe
Published: November 2022

Background

Ziprasidone is a second-generation antipsychotic drug commonly used to treat schizophrenia and bipolar disorder. Acne is a common inflammatory disease of sebaceous glands in adolescents that is often co-morbid with anxiety and depression, which may reduce treatment compliance. Through unknown mechanisms, ziprasidone may cause a range of inflammatory responses. Whether ziprasidone can cause acne in young patients with bipolar disorder has not been reported.

Case summary

We report a 23-year-old woman with a 5-year history of bipolar disorder who experienced acne during use of ziprasidone. She was admitted to our hospital during 1-month aggravation of her symptoms and was diagnosed with bipolar I disorder (current or most recent episode of depression) with psychotic features. She was given ziprasidone and soon developed acne, which she never had before; the rash worsened substantially when the ziprasidone dose was increased. At the same time, levels of inflammatory factors increased. The rash resolved after ziprasidone therapy was stopped.

Conclusion

When prescribing ziprasidone to young people with bipolar disorder, clinicians should consider the potential for adverse skin reactions. It may be useful to assay levels of inflammatory markers during ziprasidone therapy and adjust the dose if necessary in order to ensure treatment compliance.