bluuebunny/crossref_metadata_2025 huggingface dataset:
Digital Color Images as a Tool for the Sustainable Use of Embroidery Elements from Folk Costumes
Authors: Kazlacheva Zlatina , Ilieva Julieta , Dineva Petya , Stoykova Vanya , Zlatev Zlatin
Published: August 2023

The aim of the research is to improve the public’s assessment and understanding of the cultural values and history of Bulgaria. The main issues related to the sustainable use of elements of the cultural heritage are defined, and the accessible literary sources related to the digitization of the folklore heritage are reviewed. Shape indices, color, and textural characteristics were obtained from digital color images of the elements of Bulgarian folk costumes. The most informative indices of these features were selected. A kernel variant of the principal component analysis (kPCA) method was used to reduce the data volume of the feature vector. A Naïve Bayes classifier, discriminant analysis, and the support vector method (SVM) were used for classification. The classification accuracy was assessed. In the analysis of the decorative elements of Bulgarian costumes, it was found that the accuracy of classification depended both on the method for reducing the volume of data and on the separability of the classes of data, depending on the classifier used. In the analysis of microscopic images of textile fabrics from Bulgarian costumes, it was found that the accuracy of classification for the studied objects depended both on the method for reducing the volume of data and on the used classifier. In the considered cases, a classification error below 10% was obtained using a non-linear kPCA kernel and SVM with a non-linear partition function. It was proven that the results of this development can be used in the creation of modern cross-stitch patterns, textile patterns, and clothing. The practical application of these research findings has the potential to benefit various stakeholders, including cultural heritage institutions, researchers, artisans, designers, and the general public, promoting a deeper appreciation and sustainable use of costume embroidery elements. Research can continue in the direction of the sustainable use and preservation of embroidery elements of Bulgarian costumes, enriching the understanding of cultural heritage and promoting appreciation for it in future generations.
Elevated CO2 Increases Severity of Thermal Hyponasty in Leaves of Tomato
Authors: Thomas Michael D. , Heckathorn Scott A. , Boldt Jennifer K.
Published: August 2023

We previously showed that chronic warming plus elevated carbon dioxide (eCO2) causes extreme upward bending of leaflets and petioles (i.e., hyponasty) in tomato (Solanum lycopersicum), which reduces growth. In that study, only two levels of CO2 (400, 700 ppm) and temperature (30, 37 °C) were tested in young vegetative plants, and the underlying mechanism for warming + eCO2 hyponasty was not investigated. In this study, warming + eCO2 hyponasty was evaluated in tomato across a range of temperatures and CO2 concentrations, and at multiple life stages. Based on their roles in thermal hyponasty, ethylene and auxin tomato mutants were examined, and light quality manipulated, to explore the mechanism for warming + eCO2 hyponasty. At eCO2 (800 ppm), the petiole angle increased roughly linearly with temperature from 30 to 38 °C. Under high temperature stress (38 °C), the petiole angle increased similarly at all eCO2 concentrations (600/800/1000 vs. 400 ppm). All life stages examined had an increased petiole angle in leaves developed during warming + eCO2, such that most leaves in juvenile plants exhibited hyponasty but only young growing leaves did so in adults. Auxin-insensitive mutants displayed a reduced petiole angle compared to auxin-sensitive, ethylene-sensitive, ethylene-insensitive, and non-mutant genotypes, indicating that auxin, but not ethylene, is likely a main component of this hyponastic response. Reduced far-red-to-red light plus increased blue light reduced petiole hyponasty compared to non-filtered white light during warming + eCO2. These results indicate that eCO2 affects the well-characterized thermal hyponastic response of leaves, which has implications for future plant responses to climate change.
Holothurian Wall Hydrolysate Ameliorates Cyclophosphamide-Induced Immunocompromised Mice via Regulating Immune Response and Improving Gut Microbiota
Authors: Yan Chen , Qu Huiru , Li Xinli , Feng Bin
Published: August 2023

Some biologically active compounds isolated from sea cucumbers stimulate the body’s immune response by activating immune cells. Immune function is closely related to the integrity intestinal barrier and balanced gut microbiota. However, it is unknown whether the daily administration of holothurian wall hydrolysate (HWH) ameliorated intestinal dysbiosis and barrier injury induced by immunodeficiency. This study aimed to investigate the immunomodulatory effect and the underlying mechanism of HWH in cyclophosphamide (CTX)-induced immunocompromised mice. BALB/c mice received CTX (80 mg/kg, intraperitoneally) once a day for 3 days to induce immunodeficiency, and then they received the oral administration of HWH (80 or 240 mg/kg) or levamisole hydrochloride (LH, 40 mg/kg, positive control), respectively, once a day for 7 days. We utilized 16S rRNA sequencing for microbial composition alterations, histopathological analysis for splenic and colonic morphology, Western blotting for expressions of tight junction proteins (TJs), and quantitative real-time (qRT)-PCR for measurements of pro-inflammatory cytokines. HWH attenuated the immune organ damage induced by CTX, increased the secretions of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α, and promoted the recovery of goblet cells and the production of TJs (claudin-1, occludin, and ZO-1) in the colon of the immunocompromised mice. Moreover, HWH promoted the growth of beneficial microorganisms such as Lactobacillus, Lachnospiraceae, Christensenellaceae, and Bifidobacterium, while it suppressed the populations of Ruminococcus, Staphylococcus, and Streptococcus. These results demonstrate that HWH elicits intestinal mucosal immunity, repairs the damage to intestinal mucosal integrity, and normalizes the imbalanced intestinal microbial profiles in immunocompromised mice. It may be helpful to identify the biological activities of HWH to support its potential use in new prebiotics, immunomodulatory agents, and medical additives for intestinal repair.
Distinctive Features of the XBB.1.5 and XBB.1.16 Spike Protein Receptor-Binding Domains and Their Roles in Conformational Changes and Angiotensin-Converting Enzyme 2 Binding
Authors: Sharma Tej , Gerstman Bernard , Chapagain Prem
Published: August 2023

The emergence and the high transmissibility of the XBB.1.5 and XBB.1.16 subvariants of the SARS-CoV-2 omicron has reignited concerns over the potential impact on vaccine efficacy for these and future variants. We investigated the roles of the XBB.1.5 and XBB.1.16 mutations on the structure of the spike protein’s receptor-binding domain (RBD) and its interactions with the host cell receptor ACE2. To bind to ACE2, the RBD must transition from the closed-form to the open-form configuration. We found that the XBB variants have less stable closed-form structures that may make the transition to the open-form easier. We found that the mutations enhance the RBD–ACE2 interactions in XBB.1.16 compared to XBB.1.5. We observed significant structural changes in the loop and motif regions of the RBD, altering well-known antibody-binding sites and potentially rendering primary RBD-specific antibodies ineffective. Our findings elucidate how subtle structural changes and interactions contribute to the subvariants’ fitness over their predecessors.
TRIM14 Overexpression Induces Chemoresistance and Malignant Behaviors of Hepatocellular Carcinoma Cells by Activating the STAT3/HIF-1α Pathway
Authors: Xu Weiqi , Zhuang Lihong , Zhu Hongxu , Mao Anrong , Zhou Jiamin , Wang Lu
Published: August 2023

Members of the tripartite motif (TRIM)-containing protein family have been found to be involved in the progression of hepatocellular carcinoma (HCC). TRIM14 exerts a promotive impact on several cancers. This study aimed to explore the function and mechanism of TRIM14 in HCC. TRIM14 expression in HCC tissues and HCC cell lines was detected. The overexpression or knockdown model of TRIM14 was established in HCC cell lines. Cell Counting Kit-8 (CCK-8) assay, flow cytometry, Transwell assay, RT-PCR, Western blot, and immunofluorescence were performed to verify the influence of TRIM14 on cell proliferation, sensitivity to chemotherapy drugs, apoptosis, migration, invasion, and autophagy. A xenograft tumor model was used to confirm the impact of TRIM14 on tumor cell growth. As shown by the data, TRIM14 level was notably higher in the tumor tissues of HCC patients than in the adjacent tissues. The overall survival rate of patients with a high TRIM14 expression was relatively lower than that of patients with a low TRIM14 expression. TRIM14 upregulation enhanced the proliferation, autophagy, migration, and invasion of HCC cells and chemoresistant HCC cells and decreased apoptosis. TRIM14 knockdown contributed to the opposite effects. In in vivo experiments, TRIM14 upregulation bolstered tumor growth. Western blot analysis revealed that TRIM14 upregulation boosted signal transducer and activator of transcription3 (STAT3) and hypoxia-inducible factor-1alpha (HIF-1α) expression, and TRIM14 knockdown suppressed their expression. Moreover, repressing STAT3 and HIF-1α could mitigate the tumor-promoting role of TRIM14 in HCC cells. Overall, TRIM14 facilitated malignant HCC development and induced chemoresistance in HCC cells by activating the STAT3/HIF-1α axis.
Association of Thymidylate Synthase (TS) Gene Polymorphisms with Incidence and Prognosis of Coronary Artery Disease
Authors: Kim Jung Oh , Ryu Chang Soo , Lee Jeong Yong , Ko Eun Ju , Ha Yong Hyun , Sung Jung Hoon , Hwang Tae Sun , Kim In Jai , Kim Nam Keun
Published: August 2023

Coronary artery disease (CAD) is a prevalent cardiovascular condition characterized by the accumulation of plaque within coronary arteries. While distinct features of CAD have been reported, the association between genetic factors and CAD in terms of biomarkers was insufficient. This study aimed to investigate the connection between genetic factors and CAD, focusing on the thymidylate synthase (TS) gene, a gene involved in DNA synthesis and one-carbon metabolism. TS plays a critical role in maintaining the deoxythymidine monophosphate (dTMP) pool, which is essential for DNA replication and repair. Therefore, our research targeted single nucleotide polymorphisms that could potentially impact TS gene expression and lead to dysfunction. Our findings strongly associate the TS 1100T>C and 1170A>G genotypes with CAD susceptibility. We observed that TS 1100T>C polymorphisms increased disease susceptibility in several groups, while the TS 1170A>G polymorphism displayed a decreasing trend for disease risk when interacting with clinical factors. Furthermore, our results demonstrate the potential contribution of the TS 1100/1170 haplotypes to disease susceptibility, indicating a synergistic interaction with clinical factors in disease occurrence. Based on these findings, we propose that polymorphisms in the TS gene had the possibility of clinically useful biomarkers for the prevention, prognosis, and management of CAD in the Korean population.
Virus Infection and mRNA Nuclear Export
Authors: Guo Jiayin , Zhu Yaru , Ma Xiaoya , Shang Guijun , Liu Bo , Zhang Ke
Published: August 2023

Gene expression in eukaryotes begins with transcription in the nucleus, followed by the synthesis of messenger RNA (mRNA), which is then exported to the cytoplasm for its translation into proteins. Along with transcription and translation, mRNA export through the nuclear pore complex (NPC) is an essential regulatory step in eukaryotic gene expression. Multiple factors regulate mRNA export and hence gene expression. Interestingly, proteins from certain types of viruses interact with these factors in infected cells, and such an interaction interferes with the mRNA export of the host cell in favor of viral RNA export. Thus, these viruses hijack the host mRNA nuclear export mechanism, leading to a reduction in host gene expression and the downregulation of immune/antiviral responses. On the other hand, the viral mRNAs successfully evade the host surveillance system and are efficiently exported from the nucleus to the cytoplasm for translation, which enables the continuation of the virus life cycle. Here, we present this review to summarize the mechanisms by which viruses suppress host mRNA nuclear export during infection, as well as the key strategies that viruses use to facilitate their mRNA nuclear export. These studies have revealed new potential antivirals that may be used to inhibit viral mRNA transport and enhance host mRNA nuclear export, thereby promoting host gene expression and immune responses.
Towards the Standardization of Mesenchymal Stem Cell Secretome-Derived Product Manufacturing for Tissue Regeneration
Authors: Chouaib Batoul , Haack-Sørensen Mandana , Chaubron Franck , Cuisinier Frederic , Collart-Dutilleul Pierre-Yves
Published: August 2023

Mesenchymal stem cell secretome or conditioned medium (MSC-CM) is a combination of biomolecules and growth factors in cell culture growth medium, secreted by mesenchymal stem cells (MSCs), and the starting point of several derived products. MSC-CM and its derivatives could be applied after injuries and could mediate most of the beneficial regenerative effects of MSCs without the possible side effects of using MSCs themselves. However, before the clinical application of these promising biopharmaceuticals, several issues such as manufacturing protocols and quality control must be addressed. This review aims to underline the influence of the procedure for conditioned medium production on the quality of the secretome and its derivatives and highlights the questions considering cell sources and donors, cell expansion, cell passage number and confluency, conditioning period, cell culture medium, microenvironment cues, and secretome-derived product purification. A high degree of variability in MSC secretomes is revealed based on these parameters, confirming the need to standardize and optimize protocols. Understanding how bioprocessing and manufacturing conditions interact to determine the quantity, quality, and profile of MSC-CM is essential to the development of good manufacturing practice (GMP)-compliant procedures suitable for replacing mesenchymal stem cells in regenerative medicine.
Tumor Growth Ameliorates Cardiac Dysfunction and Suppresses Fibrosis in a Mouse Model for Duchenne Muscular Dystrophy
Authors: Achlaug Laris , Awwad Lama , Langier Goncalves Irina , Goldenberg Tomer , Aronheim Ami
Published: August 2023

The interplay between heart failure and cancer represents a double-edged sword. Whereas cardiac remodeling promotes cancer progression, tumor growth suppresses cardiac hypertrophy and reduces fibrosis deposition. Whether these two opposing interactions are connected awaits to be determined. In addition, it is not known whether cancer affects solely the heart, or if other organs are affected as well. To explore the dual interaction between heart failure and cancer, we studied the human genetic disease Duchenne Muscular Dystrophy (DMD) using the MDX mouse model. We analyzed fibrosis and cardiac function as well as molecular parameters by multiple methods in the heart, diaphragm, lungs, skeletal muscles, and tumors derived from MDX and control mice. Surprisingly, cardiac dysfunction in MDX mice failed to promote murine cancer cell growth. In contrast, tumor-bearing MDX mice displayed reduced fibrosis in the heart and skeletal and diaphragm muscles, resulting in improved cardiac function. The latter is at least partially mediated via M2 macrophage recruitment to the heart and diaphragm muscles. Collectively, our data support the notion that the effect of heart failure on tumor promotion is independent of the improved cardiac function in tumor-bearing mice. Reduced fibrosis in tumor-bearing MDX mice stems from the suppression of new fibrosis synthesis and the removal of existing fibrosis. These findings offer potential therapeutic strategies for DMD patients, fibrotic diseases, and cardiac dysfunction.
Clinical and Non-Clinical Cardiovascular Disease Associated Pathologies in Parkinson’s Disease
Authors: Lee Bonn , Edling Charlotte , Ahmad Shiraz , LeBeau Fiona E. N. , Tse Gary , Jeevaratnam Kamalan
Published: August 2023

Despite considerable breakthroughs in Parkinson’s disease (PD) research, understanding of non-motor symptoms (NMS) in PD remains limited. The lack of basic level models that can properly recapitulate PD NMS either in vivo or in vitro complicates matters. Even so, recent research advances have identified cardiovascular NMS as being underestimated in PD. Considering that a cardiovascular phenotype reflects sympathetic autonomic dysregulation, cardiovascular symptoms of PD can play a pivotal role in understanding the pathogenesis of PD. In this study, we have reviewed clinical and non-clinical published papers with four key parameters: cardiovascular disease risks, electrocardiograms (ECG), neurocardiac lesions in PD, and fundamental electrophysiological studies that can be linked to the heart. We have highlighted the points and limitations that the reviewed articles have in common. ECG and pathological reports suggested that PD patients may undergo alterations in neurocardiac regulation. The pathological evidence also suggested that the hearts of PD patients were involved in alpha-synucleinopathy. Finally, there is to date little research available that addresses the electrophysiology of in vitro Parkinson’s disease models. For future reference, research that can integrate cardiac electrophysiology and pathological alterations is required.
KLF10 Inhibits TGF-β-Mediated Activation of Hepatic Stellate Cells via Suppression of ATF3 Expression
Authors: Hwang Soonjae , Park Sangbin , Yaseen Uzma , Lee Ho-Jae , Cha Ji-Young
Published: August 2023

Liver fibrosis is a progressive and debilitating condition characterized by the excessive deposition of extracellular matrix proteins. Stellate cell activation, a major contributor to fibrogenesis, is influenced by Transforming growth factor (TGF-β)/SMAD signaling. Although Krüppel-like-factor (KLF) 10 is an early TGF-β-inducible gene, its specific role in hepatic stellate cell activation remains unclear. Our previous study demonstrated that KLF10 knockout mice develop severe liver fibrosis when fed a high-sucrose diet. Based on these findings, we aimed to identify potential target molecules involved in liver fibrosis and investigate the mechanisms underlying the KLF10 modulation of hepatic stellate cell activation. By RNA sequencing analysis of liver tissues from KLF10 knockout mice with severe liver fibrosis induced by a high-sucrose diet, we identified ATF3 as a potential target gene regulated by KLF10. In LX-2 cells, an immortalized human hepatic stellate cell line, KLF10 expression was induced early after TGF-β treatment, whereas ATF3 expression showed delayed induction. KLF10 knockdown in LX-2 cells enhanced TGF-β-mediated activation, as evidenced by elevated fibrogenic protein levels. Further mechanistic studies revealed that KLF10 knockdown promoted TGF-β signaling and upregulated ATF3 expression. Conversely, KLF10 overexpression suppressed TGF-β-mediated activation and downregulated ATF3 expression. Furthermore, treatment with the chemical chaperone 4-PBA attenuated siKLF10-mediated upregulation of ATF3 and fibrogenic responses in TGF-β-treated LX-2 cells. Collectively, our findings suggest that KLF10 acts as a negative regulator of the TGF-β signaling pathway, exerting suppressive effects on hepatic stellate cell activation and fibrogenesis through modulation of ATF3 expression. These results highlight the potential therapeutic implications of targeting the KLF10-ATF3 axis in liver fibrosis treatment.
A Novel Genetic Variant in MBD5 Associated with Severe Epilepsy and Intellectual Disability: Potential Implications on Neural Primary Cilia
Authors: Martins Mariana , Oliveira Ana Rafaela , Martins Solange , Vieira José Pedro , Perdigão Pedro , Fernandes Ana Rita , de Almeida Luís Pereira , Palma Paulo Jorge , Sequeira Diana Bela , Santos João Miguel Marques , Duque Frederico , Oliveira Guiomar , Cardoso Ana Luísa , Peça João , Seabra Catarina Morais
Published: August 2023

Disruptions in the MBD5 gene have been linked with an array of clinical features such as global developmental delay, intellectual disability, autistic-like symptoms, and seizures, through unclear mechanisms. MBD5 haploinsufficiency has been associated with the disruption of primary cilium-related processes during early cortical development, and this has been reported in many neurodevelopmental disorders. In this study, we describe the clinical history of a 12-year-old child harboring a novel MBD5 rare variant and presenting psychomotor delay and seizures. To investigate the impact of MBD5 haploinsufficiency on neural primary cilia, we established a novel patient-derived cell line and used CRISPR-Cas9 technology to create an isogenic control. The patient-derived neural progenitor cells revealed a decrease in the length of primary cilia and in the total number of ciliated cells. This study paves the way to understanding the impact of MBD5 haploinsufficiency in brain development through its potential impact on neural primary cilia.
Screening of Exosome-Derived Proteins and Their Potential as Biomarkers in Diagnostic and Prognostic for Pancreatic Cancer
Authors: Marin Anelis Maria , Batista Michel , Korte de Azevedo Alexandre Luiz , Bombardelli Gomig Talita Helen , Soares Caldeira Brant Rodrigo , Chammas Roger , Uno Miyuki , Dias Araújo Diogo , Zanette Dalila Luciola , Nóbrega Aoki Mateus
Published: August 2023

In the oncological area, pancreatic cancer is one of the most lethal diseases, with 5-year survival rising just 10% in high-development countries. This disease is genetically characterized by KRAS as a driven mutation followed by SMAD4, CDKN2, and TP53-associated mutations. In clinical aspects, pancreatic cancer presents unspecific clinical symptoms with the absence of screening and early plasmatic biomarker, being that CA19-9 is the unique plasmatic biomarker having specificity and sensitivity limitations. We analyzed the plasmatic exosome proteomic profile of 23 patients with pancreatic cancer and 10 healthy controls by using Nanoscale liquid chromatography coupled to tandem mass spectrometry (NanoLC-MS/MS). The pancreatic cancer patients were subdivided into IPMN and PDAC. Our findings show 33, 34, and 7 differentially expressed proteins when comparing the IPMN vs. control, PDAC-No treatment vs. control, and PDAC-No treatment vs. IPMN groups, highlighting proteins of the complement system and coagulation, such as C3, APOB, and SERPINA. Additionally, PDAC with no treatment showed 11 differentially expressed proteins when compared to Folfirinox neoadjuvant therapy or Gemcitabine adjuvant therapy. So here, we found plasmatic exosome-derived differentially expressed proteins among cancer patients (IPMN, PDAC) when comparing with healthy controls, which could represent alternative biomarkers for diagnostic and prognostic evaluation, supporting further scientific and clinical studies on pancreatic cancer.
The Role of Sensory Innervation in Homeostatic and Injury-Induced Corneal Epithelial Renewal
Authors: Feinberg Konstantin , Tajdaran Kiana , Mirmoeini Kaveh , Daeschler Simeon C. , Henriquez Mario A. , Stevens Katelyn E. , Mulenga Chilando M. , Hussain Arif , Hamrah Pedram , Ali Asim , Gordon Tessa , Borschel Gregory H.
Published: August 2023

The cornea is the window through which we see the world. Corneal clarity is required for vision, and blindness occurs when the cornea becomes opaque. The cornea is covered by unique transparent epithelial cells that serve as an outermost cellular barrier bordering between the cornea and the external environment. Corneal sensory nerves protect the cornea from injury by triggering tearing and blink reflexes, and are also thought to regulate corneal epithelial renewal via unknown mechanism(s). When protective corneal sensory innervation is absent due to infection, trauma, intracranial tumors, surgery, or congenital causes, permanent blindness results from repetitive epithelial microtraumas and failure to heal. The condition is termed neurotrophic keratopathy (NK), with an incidence of 5:10,000 people worldwide. In this report, we review the currently available therapeutic solutions for NK and discuss the progress in our understanding of how the sensory nerves induce corneal epithelial renewal.
The Contribution of TRPA1 to Corneal Thermosensitivity and Blink Regulation in Young and Aged Mice
Authors: Frutos-Rincón Laura , Luna Carolina , Aleixandre-Carrera Fernando , Velasco Enrique , Diaz-Tahoces Ariadna , Meseguer Víctor , Gallar Juana , Acosta M. Carmen
Published: August 2023

The role of TRPA1 in the thermosensitivity of the corneal cold thermoreceptor nerve endings was studied in young and aged mice. The contribution of the TRPA1-dependent activity to basal tearing and thermally-evoked blink was also explored. The corneal cold thermoreceptors’ activity was recorded extracellularly in young (5-month-old) and aged (18-month-old) C57BL/6WT (WT) and TRPA1−/− knockout (TRPA1-KO) mice at basal temperature (34 °C) and during cooling (15 °C) and heating (45 °C) ramps. The blink response to cold and heat stimulation of the ocular surface and the basal tearing rate were also measured in young animals using orbicularis oculi muscle electromyography (OOemg) and phenol red threads, respectively. The background activity at 34 °C and the cooling- and heating-evoked responses of the cold thermoreceptors were similar in WT and TRPA1-KO animals, no matter the age. Similar to the aged WT mice, in the young and aged TRPA1-KO mice, most of the cold thermoreceptors presented low frequency background activity, a low cooling threshold, and a sluggish response to heating. The amplitude and duration of the OOemg signals correlated with the magnitude of the induced thermal change in the WT but not in the TRPA1-KO mice. The basal tearing was similar in the TRPA1-KO and WT mice. The electrophysiological data suggest that the TRPA1-dependent nerve activity, which declines with age, contributes to detecting the warming of the ocular surface and also to integrating the thermally-evoked reflex blink.
The Predictive Value of the CSA Index in the Prenatal Diagnosis of Aortic Coarctation in Ultrasound Examination Performed during the Second Trimester
Authors: Zych-Krekora Katarzyna , Krekora Michał , Grzesiak Mariusz , Sylwestrzak Oskar
Published: August 2023

Background: Aortic coarctation (CoA) is the fourth most common congenital heart defect (8–10%) which occurs at a frequency of about 20–60/100,000 births. Only 22.3% of all cases appears to be diagnosed during the second trimester of pregnancy. Since the detection of prenatal aortic coarctations is very low, every effort should be made to change this situation. According to the authors of this study, the CSAi (carotid to subclavian artery index) could serve as a reliable indicator. Material and Methods: Ninety-six fetuses from healthy, single, pregnancies, with good ultrasound visualization between 18 and 27.5 weeks of gestation, and twenty-three fetuses suspected of aortic coarctation (postnatally confirmed) were included in this study. Our first aim was to compare the current most common method of prenatal CoA diagnosis based on the measurement of the aortic z-score in the aortic isthmus using the method suggested by us-CSAi. Results: Logistic regression coefficients for z-score and CSAi were analyzed as predictors of coarctation occurrence. It appears that 39.4% of coarctation occurrence can be predicted on the basis of the z-score, and 93.5% on the basis of the CSAi. The cut-off value for CSAi in the study group was 0.81 (sensitivity: 95.7%, specificity 99%). Based on the ROC curve analysis, the cut-off value for the carotid to subclavian distance (mm) was determined; the risk of coarctation increased above this value. Based on the Gini index (0.867), this value was set at 2.55 (sensitivity 82.6%, specificity 93.7%). Conclusions: CSAi measurement is currently the most sensitive method for aortic coarctation detection. For the purpose of our study, this method was applied in diagnostics in the second trimester of pregnancy. This method is easy, reproducible and should be widely introduced into everyday echocardiographic diagnostics of coarctation to minimize the risk of error.
Does Regular Practice with a “Flexible Bronchoscopy Simulator” Improve Fibreoptic Intubation Skills in Experts and Novices? A Randomized Controlled Study
Authors: van Haperen Maartje , Kemper Tom C. P. M. , Hermanides Jeroen , Eberl Susanne , Hollmann Markus W. , Breel Jennifer S. , Preckel Benedikt
Published: August 2023

Background: The appropriate management of a “difficult airway” remains a challenge for novices and experienced anaesthetists. With the current available airway technologies, e.g., video laryngoscopy, flexible bronchoscopy (fibreoptic intubation (FOI)) for endotracheal intubation is decreasing, likely diminishing caregiver skills. We investigated whether bronchoscopy simulator training improved FOI skills. Methods: 72 volunteers, consisting of anaesthetists, anaesthesia residents, and nurses, performed six exercises on a bronchoscopy simulator. At baseline and after 12 months, the six exercises included one serious game (to train agility), two basic airways, and three difficult airways. After a baseline assessment, subjects were randomly allocated to the intervention group (with) or control group without bronchoscopy simulator training every six weeks for 10 min using a preloaded serious game. The primary outcome was the difference in the time to reach the carina after 12 months, as measured objectively by the simulator. The level of stress and FOI confidence after 12 months were secondary outcomes. Results: The control and intervention groups had a similar time to reach the carina in difficult airway cases and the reported stress levels, at baseline and 12 months, showed no difference. In contrast, the intervention group’s self-reported confidence in FOI skills improved more. Conclusions: Although participants rated higher in confidence, practicing FOI skills on an airway simulator with an agility game did not increase their performance in simulated challenging airway instances.
Cytomegalovirus Disease as a Risk Factor for Invasive Fungal Infections in Liver Transplant Recipients under Targeted Antiviral and Antimycotic Prophylaxis
Authors: Breitkopf Robert , Treml Benedikt , Bukumiric Zoran , Innerhofer Nicole , Fodor Margot , Radovanovic Spurnic Aleksandra , Rajsic Sasa
Published: August 2023

Cytomegalovirus (CMV) infection is the most common opportunistic infection that occurs following orthotopic liver transplantation (OLT). In addition to the direct infection-related symptoms, it also triggers an immunological response that may contribute to adverse clinical outcomes. CMV disease has been described as a predictor of invasive fungal infections (IFIs) but its role under an antiviral prophylaxis regimen is unclear. Methods: We retrospectively analyzed the medical records of 214 adult liver transplant recipients (LTRs). Universal antiviral prophylaxis was utilized in recipients with CMV mismatch; intermediate- and low-risk patients received pre-emptive treatment. Results: Six percent of patients developed CMV disease independent of their serostatus. The occurrence of CMV disease was associated with elevated virus load and increased incidence of leucopenia and IFIs. Furthermore, CMV disease was associated with higher one-year mortality and increased relapse rates within the first year of OLT. Conclusions: CMV disease causes significant morbidity and mortality in LTRs, directly affecting transplant outcomes. Due to the increased risk of IFIs, antifungal prophylaxis for CMV disease may be appropriate. Postoperative CMV monitoring should be considered after massive transfusion, even in low-risk serostatus constellations. In case of biliary complications, biliary CMV monitoring may be appropriate in the case of CMV-DNA blood-negative patients.
Vasa, Piwi, and Pl10 Expression during Sexual Maturation and Asexual Reproduction in the Annelid Pristina longiseta
Authors: Kostyuchenko Roman P. , Smirnova Natalia P.
Published: August 2023

Naidids are tiny, transparent freshwater oligochaetes, which are well known for their ability to propagate asexually. Despite the fact that sexually mature individuals and cocoons with embryos are sometimes found in nature, in long-period laboratory cultures, worms reproduce agametically only. In this paper, we showed, for the first time, the expression of Vasa, Piwi, and Pl10 homologs in mature Pristina longiseta worms with well-developed reproductive system structures and germ cells. Although the animals have been propagated asexually by paratomic fission for over 20 years in our lab, some individuals become sexualized under standard conditions for our laboratory culture and demonstrate various stages of maturation. The fully matured animals developed a complete set of sexual apparatus including spermatheca, atrium, seminal vesicles, and ovisac. They also had a clitellum and were able to form cocoons. The cues for the initiation of sexual maturation are still unknown for P. longiseta; nevertheless, our data suggest that the laboratory strain of P. longiseta maintains the ability to become fully sexually mature and to establish germline products even after a long period of agametic reproduction. On the other hand, many of the sexualized worms formed a fission zone and continued to reproduce asexually. Thus, in this species, the processes of asexual reproduction and sexual maturation do not preclude each other, and Vasa, Piwi, and Pl10 homologs are expressed in both somatic and germline tissue including the posterior growth zone, fission zone, nervous system, germline cells, and gametes.
Blending Ethnomedicine with Modern Technology—From Conventional to Tailored Products: Modulating Biopharmaceutical Properties of Berberis Extract by Solid Lipid Nanoparticles for Wound Healing
Authors: Sharma Neetika , Vasisht Karan , Kaur Jasmine , Sandhu Simarjot Kaur , Dey Kaustav , Hameed Bakr Ahmed , Bajaj Rakesh , Kaur Indu Pal , Karan Maninder
Published: August 2023

Drug-delivery systems employing phytopharmaceuticals based on the leads in traditional knowledge offers not only an alternative but quicker and more economic strategy for drug development. Nanophytopharmaceuticals promise remarkable opportunities with the ability to overcome challenges associated with herbal medicines, such as low solubility and bioavailability, poor target specificity, and shelf life. Berberis extracts documented as Ropana (wound healer) in Sushruta Samhita are a popular traditional remedy that is amiss in the modern system of medicine as it exhibits very poor biopharmaceutical properties. Poor solubility and bioavailability necessitate the administration of high doses to achieve the desired therapeutic effects. Exploiting the diversified type of compounds with pleiotropic properties present in Berberis, the biopharmaceutical properties were engineered using an optimized freeze-dried extract and developed solid lipid nanoparticles (SLNs) as an effective drug-delivery system. An industrially viable and environment-friendly hot high-pressure homogenization technique led to a stable formulation with an average particle size of 178.4 nm, as well as a 7-fold increase in loading and a significant entrapment of 91 ± 1.25%. The pharmacodynamic studies of developed nanosystems in excision-wound models showed faster and complete healing of wounds with no scars.
Correction: Wang et al. Improve Dentin Bonding Performance Using a Hydrolytically Stable, Ether-Based Primer. J. Funct. Biomater. 2022, 13, 128
Authors: Wang Xiaohong , Yamauchi Shinobu , Sun Jirun
Published: August 2023

Jirun Sun was not included as an author in the original publication [...]
Optimization of the Number, Hub Height and Layout of Offshore Wind Turbines
Authors: Sun Haiying , Yang Hongxing , Tao Siyu
Published: August 2023

In order to make full use of the potential of wind resources in a specific offshore area, this paper proposes a new method to simultaneously optimize the number, hub height and layout of a wind farm. The wind farm is subdivided by grids, and the intersection points are set as the potential wind turbine positions. The method adopts a genetic algorithm and encodes wind farm parameters into chromosomes in binary form. The length of chromosomes is decided by the number of potential positions and the hub heights to be selected. The optimization process includes selection, crossover, and mutation, while the efficiency of wind farm is set as the optimization objective. The proposed method is validated by three benchmark cases. It has proven to be effective in deciding the number of turbines and improving the efficiency of the wind farm. Another advantage of the proposed method is that it can be widely applied to wind farms of any shape. A case study applying the new method to an irregularly shaped wind farm in Hong Kong is demonstrated. By comparing the results with the original regularly shaped wind farm, the new method can improve power generation by 6.28%. Therefore, the proposed model is a supportive tool for designing the best number, hub heights and positions of wind turbines.
Improving the Misalignment Tolerance of Wireless Power Transfer System for AUV with Solenoid-Dual Combined Planar Magnetic Coupler
Authors: Wen Haibing , Wang Peng , Li Jiayuan , Yang Jiadong , Zhang Kehan , Yang Lei , Zhao Yaopeng , Tong Xiangqian
Published: August 2023

In order to solve the problem of power transmission efficiency reduction resulting from misalignment in the Wireless Power Transfer (WPT) system for Autonomous Underwater Vehicle (AUVs), a novel coupling structure with strong tolerance to misalignment is proposed. A solenoid coil is selected as the transmitting coil, and the receiving coil is composed of dual combined planar coils. The WPT system can still maintain stable output under uncertain axial misalignments for AUVs. The magnetic field distribution of the proposed magnetic coupling structure is analyzed theoretically, and the distance between the coils in the dual combined planar receiving coil is optimized. The theoretical analysis shows that the proposed solenoid-dual combined planar coils coupling structure can effectively maintain a stable mutual inductance between the transmitting coil and receiving coil under different axial misalignments compared with solenoid-unipolar planar coil coupling structure. An S-S resonant compensated WPT experimental prototype is built to verify the output characteristics of the proposed magnetic coupling structure. Compared to the magnetic coupler with the unipolar planar coil, it is validated by experiment that the proposed magnetic coupler substantially enhances the stability of power transmission efficiency and output power when axial misalignment occurs. The power transmission efficiency decreases by 6.74% when axial misalignment increases from 0 to 40 mm in saltwater. Meanwhile, the variation of output power is less than 4.15%.
Grazing Decreases Soil Aggregation and Has Different Effects on Soil Organic Carbon Storage across Different Grassland Types in Northern Xinjiang, China
Authors: Fan Lianlian , Liang Yuanye , Li Xiaofeng , Mao Jiefei , Wang Guangyu , Ma Xuexi , Li Yaoming
Published: August 2023

Soil aggregates, as the basic component of soil, make great contributions to the stability of soil structure and soil carbon (C) sequestration. Recently, grasslands have been experiencing continuous grazing, which has had a significant impact on soil aggregation and soil C storage. However, how soil aggregates and soil C in different grasslands respond to grazing remains unclear. Therefore, three national fenced grassland-monitoring sites that represented mountain meadow (MM), temperate steppe (TS), and temperate steppe desert (TSD) were selected to investigate the differences in the responses of soil aggregates and soil C among grazing of different types of grasslands. Soil samples of 0–10 cm was collected from both inside and outside the fence of each site to analyze soil properties and soil aggregate characteristics. The results showed that soil nutrients varied greatly among the three grassland types, with the highest values in MM. At each site, grazing increased the content of sand and decreased the contents of silt and clay compared to fenced plots. Soil aggregate composition showed significant responses to both grassland type and grazing, especially the proportions of soil aggregates >2 mm, which significantly decreased by 51.7% on average in grazing plots compared with fenced plots. A significant decrease (on average, 25.1%) in the mean weight diameter (MWD) of soil aggregates under grazing was detected across all grassland types. The effect of grazing on nutrients in macroaggregates (>0.25 mm) was greater than that in microaggregates (<0.25 mm). Aggregate-associated SOC concentration decreased under grazing in MM and TS. However, grazing had no significant influence on the SOC density of MM, while it led to a significant decrease in TS and an increase in TSD. The magnitude of grazing effect size on aggregate-associated SOC varied with different soil particle sizes, with greater responses in aggregates >2 mm and the biggest value in TDS. In addition, the results of the correlation analysis and redundancy analysis (PDA) indicated that soil bulk density and nutrients made the main contribution to soil composition and stability of soil aggregates. Overall, grazing had a significant influence on soil aggregation, stability, and SOC, playing a crucial role in grassland soil stability and the accumulation of SOC.
Numerical and Experimental Study on Carbon Segregation in Square Billet Continuous Casting with M-EMS
Authors: Li Pengchao , Zhang Guifang , Yan Peng , Zhang Peipei , Tian Nan , Feng Zhenhua
Published: August 2023

Electromagnetic stirring (M-EMS) has been extensively applied in continuous casting production to reduce the quality defects of casting billets. To investigate the effect of continuous casting electromagnetic stirring on billet segregation, a 3D multi-physics coupling model was established to simulate the internal heat, momentum, and solute transfer behavior, to identify the effect of M-EMS on the carbon segregation of a continuous casting square billet of 200 mm × 200 mm. The results show that M-EMS can move the high-temperature zone upward, which is favorable for the rapid solidification of the billet, and can promote the rotational flow of the molten steel in the horizontal direction. When the electromagnetic stirring current is varied in the range of 0–500 A, the degree of carbon segregation first decreases and then increases, with the best control of segregation at 300 A. In the frequency range of 3–5 Hz, the degree of carbon segregation degree increases with frequency. Meanwhile, the simulation and experimental results show that 3 Hz + 300 A is the best electromagnetic stirring parameter for improving the carbon segregation of casting billets with a size of 200 mm × 200 mm. So, a reasonable choice of the M-EMS parameters is crucial for the quality of the billet.
Polyvinylpyrrolidone–Alginate Film Barriers for Abdominal Surgery: Anti-Adhesion Effect in Murine Model
Authors: Forysenkova Anna A. , Konovalova Mariya V. , Fadeeva Inna V. , Antonova Olga S. , Kotsareva Olga D. , Slonskaya Tatiana K. , Rau Julietta V. , Svirshchevskaya Elena V.
Published: August 2023

Surgical operations on the peritoneum are often associated with the formation of adhesions, which can interfere with the normal functioning of the internal organs. The effectiveness of existing barrier materials is relatively low. In this work, the effectiveness of soluble alginate–polyvinylpyrrolidone (PVP-Alg) and non-soluble Ca ion cross-linked (PVP-Alg-Ca) films in preventing these adhesions was evaluated. Experiments in vivo were performed on mice via mechanical injury to the adjacent peritoneum wall and the caecum, followed by the application of PVP-Alg or PVP-Alg-Ca films to the injured area. After 7 days, samples from the peritoneal wall and caecum were analyzed using histology and quantitative polymerase chain reaction (qPCR). It was shown that the expression of genes responsible for adhesion formation in the caecum in the PVP-Alg group was comparable to that in the control group, while in the PVP-Alg-Ca group, it increased by 5–10 times. These results were consistent with the histology: in the PVP-Alg group, the adhesions did not form, while in the PVP-Alg-Ca group, the adhesions corresponded to five points on the adhesion scale. Therefore, the formation of intraperitoneal adhesions can be effectively prevented by non-crosslinked, biodegradable PVP-Alg films, whereas cross-linked, not biodegradable PVP-Alg-Ca films cause inflammation and adhesion formation.
Factors Influencing the Expansion of Arch-Shaped Electromagnetic Railguns with Pre-Stressed Composite Barrels
Authors: Wang Junsheng , Xiao Jun , Huan Dajun , Yan Lei
Published: August 2023

Rail expansion significantly impacts the launch precision of a railgun system. Higher precision can be achieved when the extent of expansion is low. This paper investigates three main factors that influence the extent of rail expansion using the finite element method, including pre-stress, electromagnetic load, and stiffness of the insulators. The mean squared error between experiment results and simulating results is less than 0.06, validating the finite element model. The simulated results reveal that the extent of rail expansion increases with a decrease in pre-stress and an increase in electromagnetic pressure and the stiffness of the insulator is the most significant influencing factor, as the use of a stiff insulator not only results in a small extent of rail expansion but also delays the separation between the rails and insulators. The mechanism of how pre-stress influences the railgun system has been proposed. It has been expressed that the pre-stress maintains the integrity of the railgun system by hindering the process of a decrease in the contact surface area between rails and insulators during launch. The study provides a platform to improve the design of the railgun system.
Cyclodextrins for Lithium Batteries Applications
Authors: Desoky Mohamed M. H. , Caldera Fabrizio , Brunella Valentina , Ferrero Riccardo , Hoti Gjylije , Trotta Francesco
Published: August 2023

Due to their high energy and power density, lithium-ion batteries (LIBs) have gained popularity in response to the demand for effective energy storage solutions. The importance of the electrode architecture in determining battery performance highlights the demand for optimization. By developing useful organic polymers, cyclodextrin architectures have been investigated to improve the performance of Li-based batteries. The macrocyclic oligosaccharides known as cyclodextrins (CDs) have relatively hydrophobic cavities that can enclose other molecules. There are many industries where this “host–guest” relationship has been found useful. The hydrogen bonding and suitable inner cavity diameter of CD have led to its selection as a lithium-ion diffusion channel. CDs have also been used as solid electrolytes for solid-state batteries and as separators and binders to ensure adhesion between electrode components. This review gives a general overview of CD-based materials and how they are used in battery components, highlighting their advantages.