bluuebunny/crossref_metadata_2025 huggingface dataset:
Cybersecurity at Sea: A Literature Review of Cyber-Attack Impacts and Defenses in Maritime Supply Chains
Authors: Clavijo Mesa Maria Valentina , Patino-Rodriguez Carmen Elena , Guevara Carazas Fernando Jesus
Published: November 2024

The maritime industry is constantly evolving and posing new challenges, especially with increasing digitalization, which has raised concerns about cyber-attacks on maritime supply chain agents. Although scholars have proposed various methods and classification models to counter these cyber threats, a comprehensive cyber-attack taxonomy for maritime supply chain actors based on a systematic literature review is still lacking. This review aims to provide a clear picture of common cyber-attacks and develop a taxonomy for their categorization. In addition, it outlines best practices derived from academic research in maritime cybersecurity using PRISMA principles for a systematic literature review, which identified 110 relevant journal papers. This study highlights that distributed denial of service (DDoS) attacks and malware are top concerns for all maritime supply chain stakeholders. In particular, shipping companies are urged to prioritize defenses against hijacking, spoofing, and jamming. The report identifies 18 practices to combat cyber-attacks, categorized into information security management solutions, information security policies, and cybersecurity awareness and training. Finally, this paper explores how emerging technologies can address cyber-attacks in the maritime supply chain network (MSCN). While Industry 4.0 technologies are highlighted as significant trends in the literature, this study aims to equip MSCN stakeholders with the knowledge to effectively leverage a broader range of emerging technologies. In doing so, it provides forward-looking solutions to prevent and mitigate cyber-attacks, emphasizing that Industry 4.0 is part of a larger landscape of technological innovation.
Atrial Fibrillation in Patients with Very High Risk for Stroke and Adverse Events—Insights from the Observational ARENA Study
Authors: Alonso Angelika , Akin Ibrahim , Hochadel Matthias , Borggrefe Martin , Lesch Hendrik , Grau Armin , Zahn Ralf , Lugenbiel Patrick , Schwarzbach Christopher Jan , Sueselbeck Tim , Senges Jochen , Fastner Christian
Published: November 2024

Background: Atrial fibrillation (AF) is a major cause of stroke. An individual risk estimation remains challenging, as AF patients with and without cerebrovascular event (CVE) may differ in yet unknown factors beyond those covered by the CHA2DS2-VASc score. We aimed to identify differences between AF patients with and without CVE with regard to AF characteristics and treatment, vascular risk factors and comorbidities, prognosis and outcome. Methods: We analyzed patients included in the Atrial Fibrillation Rhine-Neckar Region (ARENA) Project, an observational cohort study of patients with AF. Patients were recruited by their general practitioner or during a hospital stay and were divided into two groups for the present analysis: patients with acute CVE at baseline and/or history of CVE versus patients without CVE. Follow-up at 1 year was conducted via phone call. Results: Of 2061 included patients (60.6% male), 292 (14.2%) belonged to the CVE group. Patients in the CVE group were older (mean age 74.6 versus 71.7 years; p < 0.001) and had a higher CHA2DS2-VASc score at baseline (5.3 versus 3.3 points; p < 0.001) based on the preceding CVE. Moreover, patients with either acute or chronic CVE had a larger left atrium (median diameter 47/46 mm versus 44 mm; p = 0.001). Patients with acute CVE had structural heart diseases (p < 0.001) less frequently than patients with previous or without CVE. Mortality at 1 year (HR 1.95; 95%-CI 1.37–2.78) was more frequent in the CVE group (p < 0.001). During 1-year of follow-up, stroke occurred more frequently in survivors with CVE (2.9% versus 0%; p < 0.001). Conclusions: AF patients with CVE have a significantly worse prognosis than AF patients without CVE. Atrial structural remodeling, underlying cardiovascular disease, stroke-induced heart injury and further unidentified factors may account for this finding. Characterization of AF patients including echocardiography to detect atrial structural remodeling may be helpful in risk stratification beyond classical scores.
Asthma Control According to GINA 2023: Does Changing the Criteria Improve Asthma Control?
Authors: Arismendi Ebymar , Ribo Paula , García Alberto , Torrego Alfons , Bobolea Irina , Casas-Saucedo Rocío , Palomino Rosa , Picado César , Muñoz-Cano Rosa , Valero Antonio
Published: November 2024

Background/Objectives: Achieving disease control is the main goal in asthmatic patients in order to prevent future risks and exacerbations. There are several clinical guidelines that set different definitions of asthma control, and these differences may affect management and treatment in many patients. Our aim was to describe asthma control patterns according to the Global Initiative for Asthma (GINA) 2023 in patients considered to have uncontrolled asthma as per previous GINA 2010 guidelines. Methods: A total of 1299 patients from the COAS study were analyzed. The COAS study was a cross-sectional multicenter study conducted in routine clinical practice that included patients with uncontrolled asthma according to GINA 2010. These patients were then re-classified using the now updated GINA 2023 asthma control criteria. Results: After applying GINA 2023 control criteria, previously uncontrolled patients were now classified as having controlled asthma in 24.3% of cases and partially controlled asthma in 16.3% of cases. Only 59.4% maintained their previous diagnosis of uncontrolled asthma. ACT in the uncontrolled patients remained similar after re-classification, as did the percentage of active smokers, respiratory allergy, rhinitis, and lung function. Conclusions: Changes in clinical guideline criteria affect the definition of asthma control. When excluding pulmonary function abnormalities in GINA 2023 asthma control criteria, the percentage of controlled patients greatly increased.
Amputation-Free Survival, WIfI Stage, and GLASS Classifications in Distal Crural or Pedal Bypass for Chronic Limb-Threatening Ischemia
Authors: Kohler Corinne , Gaizauskaite Kristina , Kotopoulos Konstantinos , Kotelis Drosos , Schmidli Jürg , Makaloski Vladimir , Weiss Salome
Published: November 2024

Background: Chronic limb-threatening ischemia (CLTI) is a severe condition with high risks of amputation and mortality, especially in patients with distal crural or pedal artery disease. Despite advances in endovascular techniques, bypass surgery remains crucial for patients with CLTI. This study aimed to investigate amputation-free survival, Wound, Ischemia, and foot Infection (WIfI) staging, and Global Limb Anatomic Staging System (GLASS) classifications in patients undergoing distal crural or pedal bypass for CLTI. Methods: This retrospective study analyzed all patients who underwent distal crural or pedal bypass for CLTI in a tertiary vascular centre from January 2010 to December 2019. The data were collected from hospital records and preoperative imaging. WIfI stages and GLASS classifications were determined for each patient, and the primary endpoint was amputation-free survival. Secondary outcomes included bypass patency, 30-day morbidity, and mortality. Results: We identified 31 bypasses performed on 29 patients with a median age of 67 years (79% male). Preoperatively, 94% of limbs were staged GLASS III and 55% were classified WIfI stage 4. Failed endovascular revascularization preceded bypass surgery in 65% of the cases. Thirty-day mortality was 3% (n = 1) and 30-day major amputation rate was 10%. Primary patency was 87%, and secondary patency was 94% at 30 days. Median duration of follow-up for survival was 59 months with a mean follow-up index (FUI) of 0.99 ± 0.05, and for major amputation and bypass patency 54 months (mean FUI 0.9 ± 0.19 and 0.85 ± 0.28, respectively). At one year, amputation-free survival was 58%, decreasing to 45% at two years, 39% at three years, and 32% at five years. Most major amputations occurred in WIfI stage 4 patients, but 53% of WIfI stage 4 and 80% of WIfI stage 3 patients were alive without major amputation after one year. Conclusions: Distal crural and pedal bypasses are essential for limb salvage in high-risk CLTI patients, particularly those with failed prior revascularization. However, the procedure is associated with limited long-term amputation-free survival. WIfI and GLASS classifications are useful for stratifying risk and guiding treatment, but outcomes suggest the need for individualized care strategies. Further research into perioperative management and alternative interventions is warranted to improve long-term outcomes in this population.
A Retrospective Analysis of Real-Life Management of Colorectal Cancer Lung-Limited Metastases Treated with Surgery: Outcomes and Prognostic Factors
Authors: Zurlo Ina Valeria , Calegari Maria Alessandra , Congedo Maria Teresa , Basso Michele , Vita Maria Letizia , Petracca Ciavarella Leonardo , Vivolo Raffaella , Anghelone Annunziato , Pozzo Carmelo , Salvatore Lisa , Meacci Elisa , Margaritora Stefano , Tortora Giampaolo
Published: November 2024

Background: Unlike liver metastases, the role of surgery in colorectal cancer lung-limited metastases (CCLLM) is not yet established, and data are still poor. We performed a retrospective analysis to evaluate the impact of surgery on the management of CCLLM. Material and Method: We retrospectively analyzed patients who received surgery for CCLLM at our Institution from January 2010 to June 2019. The aim of the study was to evaluate the impact of clinical and pathological features on the survival (OS and DFS) of patients treated with surgery for CCLLM. Results: One hundred and fifty patients were included in the analysis. Seventy-six patients received preoperative chemotherapy (pCT) and 56 an adjuvant treatment (aCT), while 18 underwent up-front surgery without CT. In the whole population, median OS (mOS) and median DFS (mDFS) were 54.1 months (95%CI 44.0–82.1) and 24.0 months (95%CI 20.0–31.2), respectively. In multivariate analysis, number of metastases was the only factor correlated to DFS (p = 0.0006) and OS (p = 0.0018). Conclusion: Our study, although retrospective and of small size, shows that tumor burden (number of metastases) is the main prognostic factor in patients undergoing lung surgery for CCLLM. Moreover, our results suggest that surgery for lung metastases might prolong survival. These data strengthen the role of multidisciplinary management to allow patients with CCLLM to pursue local treatment whenever possible, even regardless of previous liver surgery or RAS mutated status.
Does Trauma Center Volume Matter? An Analysis of Trauma Center Volume on Outcome Using the TQIP/NTDB Database
Authors: Cook Alan , Larson Nicholas J. , Altamirano Heidi M. , Ray Brittany , Pero Brandi , Mohiuddin Musharaf , Swindall Rebecca , Wadle Carly , Dries David J. , Blondeau Benoit , Rogers Frederick
Published: November 2024

Background: Increasing trauma center admission volume is said to decrease mortality. Evidence supporting this position is dependent upon patient groups and the time period studied, and gaps remain. We evaluated the effect of annual volume of critically injured patients on hospital mortality, comparing two time periods. The effect of critically injured patient volume on risk-adjusted mortality was hypothesized to decrease over time. Methods: This was a retrospective cohort study comparing data from an early group (2007–2011) and late group (2017–2021) of the National Trauma Data Bank. Critically injured adults (ISS > 15) admitted to the intensive care unit (ICU) or operating room from the emergency department at Level I and II trauma centers were included. The outcome of interest was risk-adjusted mortality across quintiles of patient admission volume, modeled using mixed-effects generalized linear models. Results: In total, 802,824 patients were included, 321,209 and 481,615 in the early and late groups, respectively. In the early group, increased patient volume was associated with a decreased risk-adjusted odds of mortality. This association was not seen in the late group. The overall odds of mortality in the late group demonstrated decreased mortality over time (OR 0.84, p < 0.001). Conclusions: The annual volume of critically injured patients was associated with decreased odds of hospital mortality during 2007–2011, though this effect was no longer present in the 2017–2021 sample. The continued dissemination of the best practices is warranted to decrease mortality, regardless of the admission volume of critically injured patients.
Characterisation of Paediatric Neuroblastic Tumours by Quantitative Structural and Diffusion-Weighted MRI
Authors: Tambasco Domenica , Zlotnik Margalit , Joshi Sayali , Moineddin Rahim , Harris Shelley , Villani Anita , Malkin David , Morgenstern Daniel A. , Doria Andrea S.
Published: November 2024

Purpose: To determine the diagnostic accuracy of quantitative diffusion-weighted (DW) MRI apparent diffusion coefficient (ADC) and tumour volumes to differentiate between malignant (neuroblastoma (NB)) and benign types of neuroblastic tumours (ganglioneuroma (GN) and ganglioneuroblastoma (GNB)) using different region-of-interest (ROI) sizes. Materials and Methods: This single-centre retrospective study included malignant and benign paediatric neuroblastic tumours that had undergone DW MRI at diagnosis. The outcome was diagnostic accuracy of the tumour volume from structural and ADC DW MRI, in comparison to histopathology (reference standard). Results: Data from 40 patients (NB, n = 24; GNB, n = 6; GN, n = 10), 18 (45%) females and 22 (55%) males, with a median age at diagnosis of 21 months (NB), 64 months (GNB), and 133 months (GN), respectively, ranging from 0 to 193 months, were evaluated. The area under the receiver operating characteristic (AUROC) curve for ADC for discriminating between neuroblastic tumours’ histopathology for a small ROI was 0.86 (95% CI: 0.75–0.98), and for a large ROI, 0.83 (95% CI: 0.71–0.96). An ADC cut-off value of 1.06 × 10−3 mm2/s was able to distinguish malignant from benign tumours with 83% (68–98%) sensitivity and 75% (95% CI: 54–98%) specificity. Tumour volume was not indicative of malignant vs. benign tumour diagnosis. Conclusions: In this study, both small and large ROIs used to derive ADC DW MRI metrics demonstrated high accuracy to differentiate malignant from benign neuroblastic tumours, with the ADC AUROC for the averaged multiple small ROIs being slightly greater than that of large ROIs, but with overlapping 95% CIs. This should be taken into consideration for standardisation of ROI-related data analysis by international initiatives.
Type-2 Inflammation in Health and Disease: Prevalence, Risk Factors and Multimorbidity
Authors: Lim Charmaine J. M. , Gross Christoph , Breyer Marie-Kathrin , Breyer-Kohansal Robab , Wouters Emiel F. M. , Hartl Sylvia
Published: November 2024

Background: In patients with airflow obstruction, the levels of biomarkers of Type-2 (T2) inflammation serve to predict the effectiveness of inhaled corticosteroid and biological therapies. Elevated biomarkers of T2 inflammation, including fractional exhaled nitric oxide (FeNO, ≥20 ppb) and blood eosinophil counts (BEC, ≥300 cells/µL), were investigated in a population-based cohort of the Austrian LEAD study. Methods: A total of 4976 individuals (aged 18–82 years) were categorised into four groups based on their FeNO and BEC levels: normal with FeNO < 20 ppb and BEC < 300 cells/µL (n = 2634); FeNO ≥ 20 ppb only (n = 1623); BEC ≥ 300 cells/µL only (n = 340); and FeNO ≥ 20 ppb and BEC ≥ 300 cells/µL (n = 379). Results: In age- and sex-adjusted regression models, individuals with elevated BEC only were most associated with chronic cough and sputum production (odds ratios [95% CI]: 1.22 [0.78, 1.84] and 1.37 [1.13, 2.62], respectively), whilst individuals with both elevated T2 biomarkers were most associated with wheezing, dyspnoea and asthma (odds ratios [95% CI]: 2.27 [1.56, 3.26], 1.32 [0.64, 2.50] and 3.63 [2.69, 4.88] respectively). Elevated levels of both FeNO and BEC presented an additive effect in extrapulmonary conditions, particularly in allergy, eczema and rhino conjunctivitis (odds ratios [95% CI]: 2.30 [1.84, 2.88], 1.37 [1.03, 1.81] and 2.95 [2.34, 3.70], respectively). Conclusions: T2 inflammation marked by elevated levels of FeNO and/or BEC is not only associated with respiratory conditions but also extends to extrapulmonary characteristics, with an additive effect.
Perineal Rectosigmoidectomy (Altemeier’s Procedure) in the Treatment of Strangulated Rectal Prolapse: A Case Series and Literature Review
Authors: Mantzoros Ioannis , Brenta Aliki , Bourtzinakou Aikaterini-Antonia , Kontaxi Ourania , Gemousakakis Georgios , Antoniou Nikolaos , Bitsianis Stefanos , Kotidis Efstathios , Kyziridis Dimitrios , Ioannidis Orestis , Kerasidou Ourania , Gkiouliava Anna , Pramateftakis Manousos , Aggelopoulos Stamatios
Published: November 2024

Background: Rectal prolapse (RP) predominantly affects women over the age of 50 and presents as mucosal, internal, or full thickness prolapse. Strangulated rectal prolapse requires immediate medical intervention, and surgical treatment options include both abdominal and perineal approaches. We aim to present a case series of perineal rectosigmoidectomy performed urgently due to strangulation and argue that Altemeier’s procedure is the preferred method for treating strangulated rectal prolapse. Methods: Perineal rectosigmoidectomy, particularly Altemeier’s procedure, is effective for incarcerated cases. Altemeier’s procedure with diverting ileostomy was used in all three patients. Results: All patients were successfully treated, with no recurrence of prolapse and stool incontinence. Conclusions: Altemeier’s procedure is ideal for the treatment of strangulated rectal prolapse.
Monitoring and Evaluation of Coastal Ecological Carrying Capacity in the Context of Sustainable Development: A Case Study of Shandong Province
Authors: Li Hao , Zhang Yuxin , Wang Xiaoli , Guo Peng , Liu Kai
Published: November 2024

The research on coastal ecological carrying capacity holds great significance for the sustainable development of coastal areas and is a focal point of the United Nations Sustainable Development Goals (SDGs). This study coupled multi-source data and ecological analysis models to construct a multi-level evaluation system and analysis method for the coastal ecological carrying capacity of Shandong Province so as to realize the dynamic monitoring and evaluation of the coastal ecological carrying capacity of Shandong Province from 2010 to 2020. The results indicated: (1) The ecological carrying capacity of the coastal zone in Shandong Province showed a “U”-shaped development trend, with 2016 being a turning point. (2) The economic development–social support system gradually became the main force driving the overall improvement of coastal ecological carrying capacity. (3) The system coupling coordination degree of ecological carrying capacity in the coastal areas of Shandong Province showed a trend of first decreasing and then increasing, with a high level of internal coupling coordination of carrying capacity. (4) Per capita GDP, environmental protection investment, per capita water resources, and other indicators were the main factors driving the changes in the ecological carrying capacity of the coastal zone. This study aims to provide methodological reference and data support for coastal ecosystem monitoring, assessment, and climate change response.
Land-Use-Change-Driven Erosion and Sediment Transport in the Yaqui River Sub-Basin (Mexico): Insights from Satellite Imagery and Hydraulic Simulations
Authors: Areu-Rangel Omar Salvador , Hernández-Hernández Miguel Ángel , Bonasia Rosanna
Published: November 2024

Soil erosion and sediment transport are significant concerns in the Yaqui River sub-basin in northwest Mexico, driven by land use changes and environmental degradation. This study aims to evaluate erosion processes between 2000 and 2020 using a combination of satellite imagery and numerical simulations with Iber software (Version 2.5.2). The primary objective is to assess the impacts of land use changes, particularly the conversion of forest to grassland, on erosion rates and sediment transport. Satellite images from 2000 and 2020 were analyzed to detect land cover changes, while Iber’s sediment transport module was used to simulate erosion patterns based on the Meyer–Peter and Müller equation for bedload transport. Hydrological and topographical data were incorporated to provide accurate simulations of flow velocity, depth, and erosion potential. The results reveal a 35.3% reduction in forest cover, leading to increased erosion and sediment transport in steep areas. Simulation predictions highlighted areas with high future erosion potential, which are at risk of further soil loss if current trends continue. Flow velocity increased, contributing to riverbank destabilization and higher sediment yield, posing a risk to infrastructure such as the Álvaro Obregón Dam. This study underscores the need for targeted erosion control measures and sustainable land management practices to mitigate future risks and protect vital infrastructure in the Yaqui River Basin.
Assessing Buffer Gradient Synergies: Comparing Objective and Subjective Evaluations of Urban Park Ecosystem Services in Century Park, Shanghai
Authors: Wei Weixuan , Wang Yiqi , Yan Qi , Liu Guanpeng , Dong Nannan
Published: November 2024

Urban parks provide essential ecosystem services (ESs) that enhance human wellbeing. However, discrepancies often arise between objective assessments of these services and stakeholders’ subjective perceptions. This study addresses a research gap concerning the synergies and tradeoffs between objective evaluations and subjective perceptions of key ecosystem services across various spatial scales. We investigated six key ecosystem services in Century Park, Shanghai, across seven buffer radii (8–100 m). Objective data were obtained from park view images (PVIs) and spatial analysis, while subjective perceptions were gathered through a scoring survey of 33 stakeholders. The key finding is that a buffer radius of 35 m offers optimal synergy between objective and subjective assessments for most ESs, particularly in pollution mediation, temperature regulation, and cultural services. Professionals showed stronger alignment in regulatory services like pollution mediation and temperature regulation, while residents exhibited higher synergy in net primary production (NPP) beyond a 75 m radius. Notably, cultural services displayed nuanced differences, with professionals preferring simpler landscapes and residents demonstrating varied aesthetic preferences. These findings emphasize the importance of integrating objective data and human perceptions in urban green space planning and governance. By incorporating diverse stakeholders and identifying optimal buffer zones, planners and designers can effectively balance ESs with human experiences. This approach ultimately fosters more sustainable and wellbeing-centered urban environments.
Development of Highway Construction Route Selection Based on Ecological Sensitivity Evaluation and Intervention Optimization Strategy Research
Authors: Yang Fan , Tang Yunlan , Xiong Suwen , Gu Chuntian , Xiao Yadan
Published: November 2024

Ecological sensitivity is an essential indicator for measuring the degree of ecological fragility of a region, and traditional highway routing ignores the ecological benefits of regional nature, which causes irreversible impacts on the natural environment. Based on the AHP method, this paper carries out a comprehensive assessment of the ecological sensitivity of the K0+000~K56+438 section of the Guanzhuang Tourist Highway in the Zhangjiajie Wuling Mountain Area from 2000 to 2020, and it utilizes the spatial autocorrelation analysis method to reveal the pattern of its spatial and temporal changes in ecological sensitivity. The following results were obtained. (1) The ecological condition of the study area is gradually improving, and the areas with higher sensitivity are mainly distributed in the northeast and southwest of the study area. (2) The ecological sensitivity of the study area has strong spatial autocorrelation, but the autocorrelation has been reduced in recent years, and the distribution of the ecological sensitivity has been gradually discretized. (3) There are problems of high ecological risk and construction difficulty in the traditional highway route selection scheme of the A line. The optimized route selection scheme, the K line, can effectively avoid highly sensitive areas by adjusting the route and bridge settings. Finally, the article proposes engineering construction suitability and ecological restoration strategies for different road sections regarding vegetation cover, landscape risk, and geological hazards. This study establishes a set of comprehensive technical chains for tourism highway routing in ecologically fragile areas based on the spatial and temporal evolution of sensitivity, which provides new ideas for traditional highway routing and is of great significance for maintaining the balance and stability of ecosystems within the road area.
MAGUS (Model for the Analysis of Geomorphological Urban Systems): From Conception to Validation on the Historic City Center of Turin (Italy)
Authors: Bernardi Asia , Licata Michele , Seitone Francesco , Fubelli Giandomenico
Published: November 2024

This study introduces and tests the MAGUS (Model for the Analysis of Geomorphological Urban Systems), a GIS-based methodological framework designed to analyze the complex interplay between urbanization and natural landscapes. Focusing on the historical evolution of Turin city center (Italy), the research employs an interdisciplinary approach to examine how urban expansion has interacted with pre-existing geomorphological features. The study leverages historical documents, digitized and georeferenced within a cartographic space, to reveal the relationships between natural and anthropogenic landforms from the Roman period through the 20th century. A key innovation of MAGUS is the integration of Multi-Temporal Markers (MTM) and Volumetric Unit of Building (VUB), which enhances the accuracy of historical data analysis. The results demonstrate that urbanization significantly alters the natural landscape in the historic city center of Turin, where the stratification of urban expansion phases partially obscures the original landform. By overlaying topographical data with historical urban expansion maps, the research highlights the concealed geomorphological elements beneath Turin’s urban fabric. This interdisciplinary study not only contributes to the field of urban geomorphology but also provides a foundation for future research into the impacts of urbanization on natural landscapes, which is increasingly critical as urban populations continue to grow.
Mechanisms of microRNA Regulation of the Epithelial–Mesenchymal Transition (EMT) in Lung Cancer
Authors: Martínez-Espinosa Israel , Serrato José A. , Cabello-Gutiérrez Carlos , Carlos-Reyes Ángeles , Ortiz-Quintero Blanca
Published: November 2024

Lung cancer remains the cancer with the highest mortality worldwide, largely due to a limited understanding of the precise molecular mechanisms that drive its progression. microRNAs (miRNAs) have emerged as crucial regulators of lung cancer progression by influencing key cellular processes, notably the epithelial–mesenchymal transition (EMT). EMT is a complex and potentially reversible process where epithelial cells lose their polarity and adhesion, reorganize their cytoskeleton, and transition to a mesenchymal phenotype, enhancing their migratory and invasive capacities. While EMT plays an essential role in normal physiological contexts such as tissue development and wound healing, it is also a critical mechanism underlying the progression and metastasis of lung cancer. This review aims to summarize the latest research findings on the role of endogenous and exosome-derived microRNAs in regulating EMT in lung cancer, focusing on studies conducted over the past five years. It also provides an overview of EMT’s essential molecular mechanisms to better understand how miRNAs regulate EMT in lung cancer.
Mitigation of Oxidized Oil Effects on Production Performances and Meat Quality of Broilers by Dietary Supplementation of Allicin
Authors: Untea Arabela Elena , Vlaicu Petru Alexandru , Varzaru Iulia , Oancea Alexandra Gabriela , Saracila Mihaela
Published: November 2024

The objective of this study was to evaluate the effects of dietary oxidized oil and allicin (two different dietary sources) as natural antioxidants on the growth performance and meat quality of broilers. A total of 200 one-day-old Ross 308 broilers were randomly divided into four dietary groups (50 birds/group). The experimental groups (OO—oxidized oil; OOA—oxidized oil and allicin; OOG—oxidized oil and garlic leaves) differed from the control one by the presence of oxidized oil in their dietary structure (peroxide value 9.07 (OO, OOA and OOG groups) vs. 1.70 (C group) meq active oxygen/kg). The diets given to the experimental groups differed from each other by the presence of allicin (100 mg/kg inclusion rate as extract (OOA) and 0.5% as garlic leaf powder (OOG)). At the end of the experiment, six animals/group were slaughtered, meat samples (breast and thigh) were collected, and nutritional value was established. The results showed that the allicin included in the experimental diet did not influence the proximate composition of breast meat (crude protein, fat, ash, and dry matter). The fatty acid profile was determined for each group of samples; a significant decrease in omega 3 FAs was noticed between the C group and the E groups (3.27% vs. 1.46%, 1.60%, and 1.56%) in breast meat samples, and a corresponding increase was noticed in saturated fatty acid (SFA) concentrations. Health indices with implications for atheroma and thrombus formation and cholesterol level were negatively affected by the presence of oxidized oil in the experimental diets, but the allicin extract supplement appeared to mitigate its influence. A positive influence of the dietary supplement was noticed on antioxidant capacity and polyphenol concentrations determined in breast and thigh samples under allicin supplement influence. The results of the current study revealed that the use of low oxidized oil in broilers diets did not affect productive performance. The nutritional quality of meat (breast and thigh) was negatively influenced by the presence of oxidized oil, but allicin supplements (extract or garlic leaves) improved lipid quality indices and antioxidant potential.
Operative Factors Associated with Severe Hypotension in the Postoperative Setting in Patients with Carotid Artery Endarterectomy
Authors: Robu Mircea , Margarint Irina-Maria , Stiru Ovidiu , Hanganu Andreea Raluca , Radulescu Bogdan , Ichim Vlad , Gorecki Gabriel-Petre , Guzu Miruna , Mazilu Claudia , Iliescu Vlad Anton , Moldovan Horatiu
Published: November 2024

Background: Carotid endarterectomy is a recognized method for preventing stroke for both symptomatic and asymptomatic hemodynamically carotid artery stenosis. Hemodynamic depression is more frequently associated with carotid artery stenting, while postoperative hypertension and cerebral hyperperfusion syndrome are known frequent complications after carotid endarterectomy. Severe hypotension after carotid revascularization is associated with a higher risk of perioperative stroke, myocardial infarction, and death, with limited data existing regarding risk factors. This study aims to investigate intraoperative risk factors for severe hypotension after carotid endarterectomy. Methods: A total of 160 patients who underwent carotid endarterectomy were included in this study. Patients with other cardiac conditions that required concomitant cardiac surgery, patients with incomplete medical records, and patients considered high risk for surgery were excluded. Results: The incidence of severe hypotension was 30.6%. Patients with severe hypotension after carotid endarterectomy had a higher incidence of diabetes, moderate mitral valve regurgitation, a history of percutaneous coronary intervention, and higher operative times. Using logistic regression, temporary shunt insertion was independently associated with severe postoperative hypotension (OR = 2.26, 95% CI = 1.09–4.71, p = 0.029). Conclusions: This result favors the selective shunting strategy when performing carotid endarterectomies, especially for those patients with comorbidities predisposing to postoperative complications.
Stable Isotope Analysis of Planktonic Lower Food Webs of Lakes Erie, Huron, Michigan and Superior
Authors: Lehman John T. , Burgess Shelby
Published: November 2024

Historical plankton samples from the St. Lawrence Great Lakes were subjected to taxon-specific 15N analysis to test the hypothesis that the changes recorded in zooplankton communities during the 21st Century are related to changes in the trophic positions of large-bodied carnivorous copepods. Daphnia mendotae was used as the reference herbivore for trophic-level comparisons. The results were that Limnocalanus macrurus, Diaptomus (Leptodiaptomus) sicilis as well as the cladoceran Bythotrephes cederstroemi show evidence of elevated carnivory compared to data from the 20th Century. The large diaptomid Diaptomus (Leptodiaptomus) sicilis has a stable isotope signature that is significantly more carnivorous in Lake Superior than in Lakes Michigan and Huron by approximately one-half trophic level. Differences were found in 10 cases out of 15 for Limnocalanus (Huron, Michigan Superior), 6 cases out of 15 for Diaptomus (Huron, Michigan) and in 1 out of 1 for Senecella (Superior). We did not find evidence to support the theory that large-bodied calanoid copepods may have improved their representation in the food webs of the upper Great Lakes by shifting their trophic position downward. Instead, large-bodied Calanoida have increased their trophic positions in parallel with their increased relative abundance. More research is thus needed to explain the driving forces for changing food web dynamics in the Great Lakes.
Intercomparison of Indexable Cutting Inserts’ Wear Progress and Chip Formation During Machining Hardened Steel AISI 4337 and Austenitic Stainless Steel AISI 316 L
Authors: Šramhauser Karel , Kraus Pavel , Špalek František , Černý Pavel , Ufitikirezi Jean de Dieu Marcel , Zoubek Tomáš , Strob Miroslav , Kononets Yevhen , Kříž Pavel , Vochozka Vladimír
Published: November 2024

This article deals with a mutual comparison of indexable cutting inserts of the CNMG 120408 type from two different manufacturers during the machining of hardened steel AISI 4337 and austenitic stainless steel AISI 316 L. The main goal is to analyse the different wear processes depending on the difference in the manufacturer’s design and also depending on the properties of the different machined materials. The progress of the wear of the main spine of the tool, the types of wear and the service life of the cutting edge were monitored, with the achievement of the critical value VBmax = 300 µm being the standard. In addition to the wear of the inserts, the production of chips was monitored in terms of their shape, average size and number of chips per 100 g of chips produced. In order to understand the relationships arising from the obtained data, an SEM equipped with an elemental analyser was used to analyse the coating layers and the substrate of the unworn inserts and the types of wear and the intensity of the surface damage of the worn inserts. A several-fold difference in the lifetime of the cutting edge was found, both in terms of design and in terms of the selected machined material, while in both cases the cutting edge with Al2O3 and TiCN layers of half thickness achieved a better result in liveness. From the point of view of chip formation, very similar results in shape and average length were observed despite the different designs of chip breakers. Cutting inserts with half the thickness of the coating layers achieved longer cutting edge life in the non-primary material application compared to the target workpiece material. At the same time, it was observed that a thinner coating layer has a positive effect on chip formation in terms of its length and shape.
Application of Hybrid Absorptive–Diffusive Panels with Variable Acoustic Characteristics Based on Wooden Overlays Designed Using Third-Degree-of-Freedom Bezier Curves
Authors: Chojnacki Bartlomiej , Schynol Kamil , Chojnacka Klara
Published: November 2024

This manuscript describes the application of novel hybrid acoustic panels with variable acoustic properties that could be used in the design process. Despite the significant growth in the modern acoustic absorbing and diffusing panel sector in recent years, there is still a need for sustainable and original designs that will fit standard interior design trends. The most significant requirement is satisfying the design needs of variable acoustic venues. The availability of acoustic panels with variable properties is minimal, as most designs are based on textiles in the form of rolling banners; therefore, there is no market diversity. The current paper presents an original solution for a novel perforated wooden panel based on third-degree-of-freedom curves. Due to the possibility of exchanging the front panel, the acoustic surface can be varied and adjusted to the room considering different requirements for the acoustic climate, for example, by modifying the attenuation range from low to mid–high frequencies. The novel panels have unique esthetic properties with functional acoustic features regarding sound diffusion and absorption. In this paper, sound absorption and diffusion measurements will be presented for the different variants of the panels, presenting the option to modify the parameters to adjust the panel’s features to the room’s needs. In situ acoustic measurements in a laboratory were conducted to test the variable acoustic panels’ influence on the room’s acoustic parameters, such as T30 and C80. In summary, the advantages of this kind of design will be discussed, alongside the possible impact on modern construction materials’ utilization in architecture.
Study and Characterisation of Bimetallic Structure (316LSI and S275JR) Made by Hybrid CMT WAAM Process
Authors: Pereira Alejandro , Alonso Antonio , Hernández Primo , Martínez Javier , Alvarez David , Wieczorowski Michal
Published: November 2024

The main objective of this research is to conduct an experimental investigation of the bimetallic material formed by 316LSI stainless steel and S275JR structural steel, produced via hybrid wire arc additive manufacturing technology with cool metal transfer welding and machining, and with the objective of being able to reduce the industrial cost of certain requirements for one of the materials. A methodological investigation has been carried out starting with welding beads of 316LSI on S275JR plates, followed by overlapping five beads and conducting final experiments with several vertical layers, with or without intermediate face milling. The results achieved optimal bead conditions for wire speeds of 4 m/min and 5 m/min at a travel speed of 400 mm/min. Overlap experiments show that the best deposition results are obtained with an overlap equal to or greater than 28%. Cooling time does not significantly influence the final geometry of the coatings. Regarding metallographic analysis, the filler material presents an austenitic columnar structure. In the base material, a bainitic structure with inferred grain refinement was detected in the heat-affected zone. An increase in hardness is observed in the heat-affected zone. In the results obtained from the tensile tests of the bimetallic material, an increase in mechanical strength and yield strength is observed in the tested specimens.
Poly-Amino-β-Cyclodextrin Microparticles for the Reduction of Xenobiotics and Emerging Contaminants, Including Pharmaceuticals, from the Natural Environment
Authors: Ciesielski Wojciech , Kulawik Damian , Girek Beata , Kozieł-Trąbska Kinga , Zawierucha Iwona , Girek Tomasz
Published: November 2024

The contamination of the natural environment by xenobiotics and emerging contaminants, including pharmaceuticals, poses significant risks to ecosystems and human health. Among these contaminants, hormones and pharmaceutical compounds are particularly concerning due to their persistence and potential biological effects even at low concentrations. In this study, we investigated the efficacy of poly-amino-β-cyclodextrin (PA-β-CD) microparticles in adsorbing and reducing specific xenobiotics and pharmaceuticals from aqueous solutions. Our research focused on four contaminants: two hormones, testosterone and progesterone, and two pharmaceutical drugs, diclofenac and carbamazepine. High-performance liquid chromatography (HPLC) was employed to quantify the adsorption capacity and efficiency of PA-β-CD microparticles.
Biotechnological Tool for Metal(loid)s as Cd, Cu, Ni, and P Management with Multiple Approaches: Bioremediation, Recovery of Raw Materials, and Food Safety
Authors: Scotti Adalgisa , Castaño Gañan Ana Rosa , Silvani Vanesa Analía , Juarez Andrea , Coria Gabriela , Utge Perri Sofía , Colombo Roxana Paola , García-Romera Inmaculada , Izaguirre-Mayoral María Luisa , Godeas Alicia , Ubaldini Stefano
Published: November 2024

Contaminated soils are a challenge for implementing biotechnology in bioremediation, the recovery of Critical and Strategic Raw Materials (CRMs and SRMs), and food security. European Union (EU) Governments have established strict limits on As, Pb, Cd, and Hg in foods (Document 32023R0915) and requested the recovery of 34 CRMs within a circular economy (CE) (5th CRMs list). This study proposed a biotechnological tool for the decontamination of soil with heavy metal(loid)s by arbuscular mycorrhizal (AM)-assisted phytoextraction and the subsequent recovery of CRMs or by phytostabilization to prevent their entry into the food chain. It consisted of placing Baccharis salicifolia plants, inoculated or non-inoculated with AM fungi, into bioreactors (BRs) containing mining soil with Cd, Ni, and Cu, according to the Argentinian Patent (AR090183B1). The bioextractive potential (BP) was also estimated at the highest Technological Readiness Level (TRL) using a vegetable depuration module (VDM, TRL 6). Inoculated plants showed significantly higher aerial bioaccumulation coefficients (Cd: 68.62; P: 2.99; Ni: 2.51; Cu: 0.18) in BRs, and the BP values reached 1.16 g, 9.75 g, 2.40 g, and 213.1 g for Ni, Cd, Cu, and P, respectively. Finally, these CRMs and SRMs could be recovered from biomass through hydrometallurgy within a CE framework.
Evaluation of Single-Event Effect Current-Carrier Mapping Based on Experimental Data
Authors: Bao Mengtian , Wang Ying , Yang Jianqun , Li Xingji
Published: November 2024

For single-event radiation damage of power MOSFET devices, this paper aims to establish a statistical analysis method based on external observation (gate/drain current characteristics in irradiation environment) to recognize and evaluate the radiation evolution process and damage mechanism of microscopic physical quantities inside the devices, namely current-carrier (CC) mapping. Firstly, a special data fluctuate–collapse transform analysis method is proposed according to the temporal characteristics of the gate/drain current. Secondly, a carrier dynamic balance ratio based on current data is defined to evaluate the radiation damage degree of the device. TCAD is used to deeply study the relationship between the external current characteristics and the evolution process of internal physical quantities and the damage mechanism. The results show that the current data timing analysis based on fluctuate–collapse transformation can better peer into the evolution process of irradiation events inside the device, and the statistical analysis based on the dynamic balance ratio of carriers can evaluate the severity of irradiation damage to a certain extent.
Impact of Temperature and Substrate Type on the Optical and Structural Properties of AlN Epilayers: A Cross-Sectional Analysis Using Advanced Characterization Techniques
Authors: Wei Wenwang , Peng Yi , Hu Yuefang , Xu Xiuning , Xie Quanwen
Published: November 2024

AlN, with its ultra-wide bandgap, is highly attractive for modern applications in deep ultraviolet light-emitting diodes and electronic devices. In this study, the surface and cross-sectional properties of AlN films grown on flat and nano-patterned sapphire substrates are characterized by a variety of techniques, including photoluminescence spectroscopy, high-resolution X-ray diffraction, X-ray photoelectron spectroscopy, ultraviolet photoelectron spectroscopy, and Raman spectroscopy. The results indicate that different sapphire substrates have minimal impact on the photoluminescence spectrum of the epitaxial films. As the temperature increased, the radius of curvature of the AlN films increased, while the warpage decreased. The AlN films grown on nano-patterned substrates exhibited superior quality with less surface oxidation. During the growth of AlN thin films on different types of substrates, slight shifts in the energy bands occurred due to differences in the introduction of carbon-related impurities and intrinsic defects. The Raman shift and full width at half maximum (FWHM) of the E2(low), A1(TO), E2(high), E1(TO), and E1(LO) phonon modes for the cross-sectional AlN films varied with the depth and temperature. The stress state within the film was precisely determined with specific depths and temperatures. The FWHM of the E2(high) phonon mode suggests that the films grown on nano-patterned substrates exhibited better crystalline quality.
Delayed Effect of Superheated Steam Treatment on Starch Retrogradation of Rice Cake After Storage by Modifying Starch Chain-Length Distribution in Rice Flour
Authors: Cao Ruge , Zhang Zixiu , Wang Xishuang , Xia Chen , Cheng Yongqiang , Wang Zhiwei , Qiu Ju
Published: November 2024

This study investigated the effects of superheated steam (SS) treatment on the physicochemical properties of rice flour and the subsequent impact on rice cake quality. The SS-180 resulted in higher final viscosity and significantly increased pasting time and the temperature of rice flour (p < 0.05). A significant enhancement in the water holding capacity of rice flour (p < 0.05) was due to the rice starch aggregated in this SS process. SS treatment also led to an increase in the proportion of short chains of amylopectin in rice flour from 30.40% to 37.59%, while a decrease in long chains retarded retrogradation and improved rice cake texture. The hardness of the SS-treated rice cake was lower than that of the untreated one, but the specific volume was increased significantly (p < 0.05). After 7 days of storage, rice cake with SS-180 treatment showed the lowest hardness, namely, the lowest retrograded process. These findings underscore the potential of SS treatment to enhance the physicochemical properties of rice flour and improve rice cake quality.