bluuebunny/crossref_metadata_2025 huggingface dataset:
Seasonal Surges in Bacterial Diversity along the Coastal Waters of the Eastern Arabian Sea
Authors: Hafza S. , Parvathi A. , Pradeep Ram A. S. , Alok Thampan K. , Neeraja R. , Jyothibabu R. , Gupta G. V. M.
Published: October 2024

The upwelling phenomenon plays a vital role within marine ecosystems, transporting essential nutrients from the bottom to the surface and boosting biological productivity. However, the bacterial community structure in upwelling zones along the western coast of India (WCI) is understudied. This research systematically examines bacterial diversity across three seasons—pre-monsoon (PR), monsoon (MN), and post-monsoon (PM)—using next-generation sequencing. Our findings show distinct spatial patterns of bacterial communities in the Arabian Sea and demonstrate that ecological variations influence bacterial distribution in this dynamic environment. During MN, the bacterial community exhibited greater species diversity but lower overall abundance compared to PR and PM. Non-Metric MDS cluster analysis revealed a 78% similarity (at order level) between PR and PM, indicating that MN supports unique bacterial diversity. KEGG analysis showed significant seasonal variations in metabolic functions, with increased functional potential during MN. Additionally, Carbohydrate-Active enZymes (CAZymes) analysis revealed distinct seasonal profiles, among which the GH13 enzymes were the most prevalent glycoside hydrolases during MN, predominantly being sucrose phosphorylase and glucosidase, known for breaking down glucan deposits derived from phytoplankton. The CAZymes profiles supported taxonomic and KEGG pathway findings, reinforcing that microbial communities are seasonally distinct and functionally adapted to changing availability of nutrients.
Correlation between Human Embryo Morphokinetics Observed through Time-Lapse Incubator and Life Birth Rate
Authors: Maghiar Laura , Naghi Petronela , Zaha Ioana Alexandra , Sandor Mircea , Bodog Alin , Sachelarie Liliana , Vieriu Georgiana , Stefan Liana , Huniadi Anca , Hurjui Loredana Liliana
Published: October 2024

(1) Background: Does the variation in sequential development times of embryos, observed through time-lapse monitoring, between the two study groups play a role in predicting pregnancy success? (2) Methods: The prospective double-arm study was to identify the morphokinetic parameters specific to embryos that were capable of implanting and were conducted on 89 embryos cultured in the Esco Miri time-lapse incubator, divided into two groups: Lot A, consisting of 57 embryos that successfully implanted and resulted in life birth rate (LBR), and Lot B (NLB), comprising 32 embryos that did not implant, leading to a negative beta-hCG outcome. (3) Results: Baseline characteristics, including female age, were not found to be statistically significant (p > 0.01). In contrast, there is a highly statistically significant difference concerning oocytes (p = 0.0029). Morphokinetic variables represented by sequential culture times were not statistically significant (p > 0.01) when comparing the two groups. However, the negative mean differences between these parameters suggest that the times for Lot A are better (shorter) than those for Lot B. While not statistically significant, these differences may still have practical significance. In the case of grading, the difference is considered to be extremely statistically significant (p < 0.01). (4) Conclusions: Although there are no statistically significant differences in sequential timings (p > 0.01) between the two groups, there are parameters indicating predictive potential for exploring pregnancy in embryo morphokinetics.
AI-ADC: Channel and Spatial Attention-Based Contrastive Learning to Generate ADC Maps from T2W MRI for Prostate Cancer Detection
Authors: Ozyoruk Kutsev Bengisu , Harmon Stephanie A. , Lay Nathan S. , Yilmaz Enis C. , Bagci Ulas , Citrin Deborah E. , Wood Bradford J. , Pinto Peter A. , Choyke Peter L. , Turkbey Baris
Published: October 2024

Background/Objectives: Apparent Diffusion Coefficient (ADC) maps in prostate MRI can reveal tumor characteristics, but their accuracy can be compromised by artifacts related with patient motion or rectal gas associated distortions. To address these challenges, we propose a novel approach that utilizes a Generative Adversarial Network to synthesize ADC maps from T2-weighted magnetic resonance images (T2W MRI). Methods: By leveraging contrastive learning, our model accurately maps axial T2W MRI to ADC maps within the cropped region of the prostate organ boundary, capturing subtle variations and intricate structural details by learning similar and dissimilar pairs from two imaging modalities. We trained our model on a comprehensive dataset of unpaired T2-weighted images and ADC maps from 506 patients. In evaluating our model, named AI-ADC, we compared it against three state-of-the-art methods: CycleGAN, CUT, and StyTr2. Results: Our model demonstrated a higher mean Structural Similarity Index (SSIM) of 0.863 on a test dataset of 3240 2D MRI slices from 195 patients, compared to values of 0.855, 0.797, and 0.824 for CycleGAN, CUT, and StyTr2, respectively. Similarly, our model achieved a significantly lower Fréchet Inception Distance (FID) value of 31.992, compared to values of 43.458, 179.983, and 58.784 for the other three models, indicating its superior performance in generating ADC maps. Furthermore, we evaluated our model on 147 patients from the publicly available ProstateX dataset, where it demonstrated a higher SSIM of 0.647 and a lower FID of 113.876 compared to the other three models. Conclusions: These results highlight the efficacy of our proposed model in generating ADC maps from T2W MRI, showcasing its potential for enhancing clinical diagnostics and radiological workflows.
The Gains and Losses of Cultivated Land Requisition–Compensation Balance: Analysis of the Spatiotemporal Trade-Offs and Synergies in Ecosystem Services Using Hubei Province as a Case Study
Authors: He Qingsong , Jiang Xu , Zhang Yang
Published: October 2024

The cultivated land requisition–compensation balance (CLRCB) policy is an important policy implemented by China to address the reduction in cultivated land and ensure food security. Although this policy has alleviated the loss of cultivated land quantity, it has had complex and diverse impacts on ecosystem services. Taking Hubei Province as the study area, this research explores the impact of the implementation of the CLRCB on ecosystem services and simulates the changes in ecosystem services in the study area in 2030 and the impact of CLRCB on the interactions among various services. The results show the following: (1) from 2000 to 2020, Hubei Province achieved a balance in the quantity of cultivated land through excessive compensation but failed to reach the goals of balancing cultivated land yield and productivity. (2) During the requisition–compensation process, habitat quality decreased by 501,862, and carbon storage lost 1.3 × 107 t, indicating negative ecological impacts; soil conservation services increased by 184.2 × 106 t, and water production increased by 21.29 × 108 m3. Within the cultivated land requisition–compensation area, habitat quality and carbon storage, as well as soil conservation and water production, exhibited synergistic relationships, while the remaining pairs of ecosystem services showed trade-off relationships. (3) The simulation of ecosystem services in 2030 indicates that soil conservation and water production are highest under the natural development scenario, while habitat quality and carbon storage are highest under the ecological protection scenario, both of which are superior to the urban development scenario. Under the natural development scenario, the trade-off and synergistic relationships among various ecosystem services in the cultivated land requisition–compensation area remain unchanged, while these relationships change significantly under the other two scenarios. This study emphasizes that future CLRCB should not only focus on maintaining the quantity of cultivated land but also consider the comprehensive benefits of ecosystem services, in order to achieve sustainable land-use management and ecological conservation.
Is LCZ Enough? Physical Properties, Thermal Environments and Cooling Effects of Green Roofs in High-Density Urban Industrial Blocks
Authors: Luo Tianqing , Liu Yifan , Zhai Yujia , Gong Xiuqi
Published: October 2024

With rapid worldwide urbanization, the urban heat island (UHI) effect is becoming more and more serious. The UHI effect is more intense in industrial areas. Green roofs are an effective way to mitigate UHIs in high-density cities, which calls for thorough examination. This study explored the associations between the block characteristics and block thermal environment in high-density industrial areas based on the widely accepted Local Climate Zone (LCZ) scheme. The pedestrian air temperature comparisons before and after virtual green roof installations presented the cooling effects of green roofs. Thirty-six typical industrial blocks were involved in the study and the simulations were conducted utilizing ENVI-met. The results showed that (1) the air temperature in LCZ4 is significantly lower than those in LCZ2 and LCZ6, but no significant differences were identified between other pairs of LCZ types; (2) the cooling effect of green roofs significantly differs among LCZs, and is associated with sky view factor (SVF), average building area (ABA) and average building shape index (ABSI); (3) in high-density urban areas, additional functional parameters and building-volume indices should be included to better address the physical characteristics, thermal environment, and green roof cooling effect of industrial blocks. This study could improve the validity of LCZ classification for high-density industrial blocks and may provide direct implications for green roof planning.
Rapid Appraisal of Wildlife Corridor Viability with Geospatial Modelling and Field Data: Lessons from Makuyuni, Tanzania
Authors: Lyimo Emmanuel H. , Mayengo Gabriel , Hariohay Kwaslema M. , Holler Joseph , Kisingo Alex , Castico David J. , Kimambo Niwaeli E. , Lucas Justin , Martin Emanuel H. , Nguma Damian
Published: October 2024

Connectivity between protected areas is necessary to prevent habitat fragmentation. Biodiverse countries like Tanzania craft legislation to promote habitat connectivity via the creation of ecological corridors, but their viability for wildlife often remains unknown. We therefore develop a scalable and replicable approach to assess and monitor multispecies corridor viability using geospatial modeling and field data. We apply and test the approach in the Makuyuni study area: an unprotected ecological corridor connecting Tarangire National Park to Essmingor mountain, Makuyuni Wildlife Park and Mto Wa Mbu Game Controlled Area. We analyzed the viability of Makuyuni as an ecological corridor by creating and validating a geospatial least-cost corridor model with field observations of wildlife and livestock. We created the model from publicly available spatial datasets augmented with manual digitization of pastoral homesteads (bomas). The least-cost corridor model identified two likely pathways for wildlife, confirmed and validated with field observations. Locations with low least-cost values were significantly correlated with more wildlife observations (Spearman’s rho = −0.448, p = 0.002). Our findings suggest that Makuyuni is a viable ecological corridor threatened by development and land use change. Our methodology presents a replicable approach for both monitoring Makuyuni and assessing corridor viability more generally. The incorporation of manually digitized homesteads (bomas) and field-based livestock observations makes corridor assessment more robust by taking into account pastoral land uses that are often missing in land cover maps. Integration of geospatial analysis and field observations is key for the robust identification of corridors for habitat connectivity.
The Influence of the Gut Microbiota on Host Health: A Focus on the Gut–Lung Axis and Therapeutic Approaches
Authors: Alswat Amal S.
Published: October 2024

The human gut microbiota is a complex ecosystem harboring thousands of microbial strains that play a crucial role in maintaining the overall well-being of its host. The composition of the gut microbiota varies with age from infancy to adulthood and is influenced by dietary habits, environment, and genetic disposition. Recent advances in culture-independent techniques and nucleic acid sequencing have improved our understanding of the diversity of the gut microbiota. The microbial species present in the gut release short-chain fatty acids (SCFAs), which have anti-inflammatory properties. The gut microbiota also plays a substantial role in modulating the host′s immune system, promoting immune tolerance, and maintaining homeostasis. The impact of the gut microbiota on the health of the host is quite evident, as gut dysbiosis has been linked to various diseases, including metabolic disorders, autoimmune diseases, allergies, and inflammatory bowel diseases. The gut microbiota has bidirectional communication with the respiratory system, creating the gut–lung axis, which has been associated with different respiratory diseases. Therapeutic approaches targeting the gut microbiota, such as probiotics, prebiotics, dietary interventions, and fecal microbiota transplantation (FMT), aim to restore microbial balance and promote the growth of beneficial strains in the gut. Nonetheless, gaining knowledge of the complex interactions between the gut microbiota and the host is necessary to develop personalized medicine approaches and microbiota-based therapies for various conditions. This review summarizes studies related to the gut–lung axis with particular emphasis on the role of the microbiota. Future research directions are also discussed.
New Role of the Serotonin as a Biomarker of Gut–Brain Interaction
Authors: Liu Hong Nian , Nakamura Masanao , Kawashima Hiroki
Published: October 2024

Serotonin (5-hydroxytryptamine: 5-HT), a neurotransmitter that regulates mood in the brain and signaling in the gut, has receptors throughout the body that serve various functions, especially in the gut and brain. Selective serotonin reuptake inhibitors (SSRIs) are used to treat depression, but their efficacy is uncertain. Depression is often associated with early gastrointestinal symptoms. Gut disorders such as functional dyspepsia (FD), irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), are linked to elevated serotonin levels. In this review, we would like to discuss the approach of using serotonin as a biomarker for gut–brain, and body-wide organ communication may lead to the development of preventive and innovative treatments for gut–brain disorders, offering improved visibility and therapeutic monitoring. It could also be used to gauge stress intensity for self-care and mental health improvement.
Quality, Safety, and Security Systems in the Greek Port Industry: Over Twenty Years of Research, Empirical Evidence, and Future Perspectives
Authors: Chlomoudis Constantinos , Kostagiolas Petros , Pallis Petros , Platias Charalampos
Published: October 2024

Background: Quality, Safety and Security are embedded in all aspects of port operations and are crucial for port industry stakeholders. Over the past decades, numerous assurance systems, codes, and regulations for quality, safety, and security have been developed and implemented in ports world-wide. This paper examines key insights for the implementation those systems in ports, reviewing over 20 years of empirical research on the impact and outcomes of such systems in Greek ports and internationally. Methods: It compares and discusses evidence from two empirical surveys spanning the first two decades of the 21rst century. The first survey was conducted in 2011 (with evidence from 2000 to 2011) including 12 major Greek ports (SAs) and the second one was conducted up to 2022–2023, including 23 over 25 Greek ports (the same SAs and other ones from the Greek TEN-T network). Results: The higher-level scope of this paper is to investigate critical perspectives on and trends in quality, safety, and security systems in the port industry. Conclusions: This investigation aims to strengthen the assumption that quality, safety, and security play a pivotal role in shaping the image, performance, and growth of ports.
Effects of Strontium Modification on Corrosion Resistance of Al-Si Alloys in Various Corrosive Environments
Authors: Jie Lau Lin , Baig Mirza Farrukh , Mhd Noor Ervina Efzan
Published: October 2024

This study investigates the impact of strontium (Sr) additions on the corrosion resistance of an LM6 (A413) aluminium alloy. By incorporating varying concentrations of Sr (0.01 wt.% and 0.05 wt.%), the morphological and corrosion behaviours of the alloy were analysed under different corrosive environments, including sulphuric acid, sodium hydroxide, and sodium chloride solutions. The results demonstrate that Sr modifications significantly enhance the alloy’s corrosion resistance, with the most substantial improvement observed at 0.05 wt.% Sr. The analysis revealed that the weight loss of the alloy in sulphuric acid decreased by 2.5% with 0.05 wt.% Sr after 10 days of immersion, due to the formation of a stable passive oxide layer. In sodium hydroxide, however, the weight loss was reduced by 5% with 0.05 wt.% Sr after 10 days, indicating aggressive uniform corrosion. In the 3.5% sodium chloride solution, the corrosion rates remain relatively low, and the 0.05 wt.% Sr alloy showed a decrease in corrosion product formation over time, suggesting enhanced resistance. Detailed surface analyses, including 3D profiling and morphology assessments, revealed that Sr additions refine the eutectic silicon phase, transforming it from a coarse to a more desirable fibrous or lamellar structure, thus improving the alloy’s overall performance. The innovative findings underscore the potential of Sr as an effective microstructural modifier for enhancing the durability and longevity of Al-Si alloys in corrosive environments.
Electro-Optic Kerr Response in Optically Isotropic Liquid Crystal Phases
Authors: Yevchenko Tetiana , Dardas Dorota , Bielejewska Natalia , Brańka Arkadiusz C.
Published: October 2024

The results of an experimental investigation of the temperature and wavelength dependence of the Kerr constant (K) of mixtures with an increasing amount of chiral dopant in an isotropic liquid crystal phase are reported. The material was composed of a nematic liquid crystal (5CB) and a chiral dopant (CE2), which formed non-polymer-stabilized liquid crystalline blue phases with an exceptionally large value of K∼2 × 10−9 mV−2. The measurements were performed on liquid and blue phases at several concentrations covering a range of temperatures and using three wavelengths: 532 nm, 589 nm and 633 nm. The work focused on changes caused by concentration and their impact on the increase in the value of K, and it was found that in the case of the 5CB/CE2 mixture these changes were significant and quite systematic with temperature and wavelength. It is shown that the dispersion relation based on the single-band birefringence model described K well in isotropic liquid crystal phases at all of the measured concentrations. In an isotropic fluid, both temperature-dependent parameters in the dispersion relation had a simple linear form and, therefore, the K-surface could be described by only four constants. In the blue phase, the expression reproducing the temperature variation of K depended on concentration, which could vary from being almost linear to quasi-linear and could be represented well by an inverse exponential analytic expression.
Optimisation of Active Magnetic Elements in Beam-like Structures—Numerical Modelling Studies
Authors: Majewska Katarzyna
Published: October 2024

This paper explores integrating advanced materials, including magnetic shape memory alloys, magnetorheological fluids, and classical shape memory alloys, within structural elements to achieve exceptional physical properties. When these materials are integrated within structures—whether as wires, actuators, or dampers—they provide the structures with unique static, dynamic, and damping characteristics not commonly found in nature. This study aimed to evaluate the efficacy of these active materials in enhancing the performance of beam-like structures. This investigation was conducted through a comprehensive numerical analysis, focusing on a composite beam. The study examined the impact of different active elements, their position within the structure, and their influence on key dynamic properties. Additionally, a simplified damage scenario was considered, wherein the adverse effects of structural damage were mitigated through the strategic application of these materials. Numerical simulations were carried out using the finite element method, with custom computational codes developed in MATLAB. The findings of these simulations are presented and discussed in this paper.
Synthesis of β-Ga2O3:Mg Thin Films by Electron Beam Evaporation and Postannealing
Authors: Fan Weitao , Li Sairui , Ren Wei , Yang Yanhan , Li Yixuan , Liu Guanghui , Wang Weili
Published: October 2024

Doping divalent metal cations into Ga2O3 films plays a key role in adjusting the conductive behavior of the film. N-type high-resistivity β-Ga2O3:Mg films were prepared using electron beam evaporation and subsequent postannealing processing. Various characterization methods (X-ray diffraction, X-ray photoelectron spectroscopy, photoluminescence, etc.) revealed that the Mg content plays an important role in affecting the film quality. Specifically, when the Mg content in the film is 3.6%, the S2 film’s resistivity, carrier content, and carrier mobility are 59655.5 Ω·cm, 1.95 × 1014 cm3/C, and 0.53682 cm2/Vs. Also, the film exhibits a smoother surface, more refined grains, and higher self-trapped exciton emission efficiency. The Mg cation mainly substitutes the Ga+ cation at a tetrahedral site, acting as a trap for self-trapped holes.
Research on the Physical Properties of an Eco-Friendly Layered Geopolymer Composite
Authors: Przybek Agnieszka , Łach Michał
Published: October 2024

Building envelopes with natural fibers are the future of sustainable construction, combining ecology and energy efficiency. The geopolymer building envelope was reinforced with innovative composite bars and two types of natural insulation (coconut mats and flax/hemp non-woven fabrics) were used as the core material. A 10 mol sodium hydroxide solution with an aqueous sodium silicate solution was used for the alkaline activation of the geopolymers. The purpose of this study was to confirm the feasibility of producing geopolymer composites with insulating layers made of renewable materials, which would have compressive strengths like those of C25/30-grade concrete and thermal conductivity coefficients like those of lightweight concrete. This publication presents the results of physicochemical tests on the base materials (oxide (XRF) and mineral phase (XRD) analysis as well as morphology and EDS) and studies the physical (density measurements), mechanical (flexural and compressive strength tests) and insulating properties (thermal conductivity measurements) of the finished sandwich partitions. The composites achieved a flexural strength of 7 MPa, a compressive strength of up to 30 MPa and a decrease in the thermal conductivity coefficient of about 60%. The research demonstrates contribution to sustainable construction by developing geopolymer composites, offering both structural integrity and superior thermal insulation. This innovation not only reduces reliance on traditional, carbon-intensive materials but also promotes the use of eco-friendly resources, significantly lowering the carbon footprint of construction. The integration of natural fibers into geopolymer matrices addresses key environmental concerns, advancing a rapidly growing field that aligns with global efforts toward energy efficiency, waste reduction, and circular economy principles in building design.
A Kinetic Model for Oxide–Carbonitride Inclusion Heterogeneous Nucleation and Precipitation during Superalloy Solidification
Authors: Zhao Peng , Yang Shulei , Gu Yu , Liu Wei , Yang Shufeng
Published: October 2024

Complex oxide–carbonitrides (MgO-Ti(CN), Al2O3-Ti(CN), and MgO·Al2O3-Ti(CN)) are the most common non-metallic inclusions presented in cast and wrought superalloys. In this work, a coupled kinetics model was proposed to predict the complex oxide–carbonitride inclusion’s precipitation behavior during the solidification of superalloys. This model takes into account thermodynamics, micro-segregation, heterogeneous nucleation in the inter-dendritic liquid, and growth controlled by the diffusion of solute elements and kinetics of interfacial reaction. The results demonstrated that both the cooling rate and nitrogen content take significant effects on the final size of complex oxide–carbonitride inclusions, as the former controls the total growth time and the latter determines the initial precipitation temperature. In comparison, the particle size of primary oxides shows a negligible impact on the final size of complex inclusions. The practice of an industrial vacuum arc remelting confirmed that the inclusion size variation predicted by the present model is reasonably consistent with the experimental results.
Rapid Construction of Liquid-like Surfaces via Single-Cycle Polymer Brush Grafting for Enhanced Antifouling in Microfluidic Systems
Authors: Wu Feng , Xu Jing , Liu Yuanyuan , Sun Hua , Zhang Lishang , Liu Yixuan , Wang Weiwei , Chong Fali , Zou Dan , Wang Shuli
Published: October 2024

Liquid-like surfaces have demonstrated immense potential in their ability to resist cell adhesion, a critical requirement for numerous applications across various domains. However, the conventional methodologies for preparing liquid-like surfaces often entail a complex multi-step polymer brush modification process, which is not only time-consuming but also presents significant challenges. In this work, we developed a single-cycle polymer brush modification strategy to build liquid-like surfaces by leveraging high-molecular-weight bis(3-aminopropyl)-terminated polydimethylsiloxane, which significantly simplifies the preparation process. The resultant liquid-like surface is endowed with exceptional slipperiness, effectively inhibiting bacterial colonization and diminishing the adherence of platelets. Moreover, it offers promising implications for reducing the dependency on anticoagulants in microfluidic systems constructed from PDMS, all while sustaining its antithrombotic attributes.
Design, Testing, and Experimental Validation of a Rotary Vibration-Assisted Polishing Device (RVAPD) for Enhanced Machining and Surface Quality
Authors: Liu Silin , Gu Yan , Lin Jieqiong , Xu Zisu , Gao Tianyu , Liu Xinyang , Zhang Xiaoming , Yu Bingjin
Published: October 2024

A rotary vibration-assisted polishing device (RVAPD) is designed to enhance polishing force by converting PZT’s linear motion into the rotary motion of a central platform via a flexible mechanism, improving material surface quality. The RVAPD is optimized, simulated, and tested to meet high-frequency and large-amplitude non-resonant vibration polishing requirements. Its structure, designed using theoretical models and finite element software, offers a wide range of polishing parameters. Performance parameters are validated through open-loop tests, confirming effectiveness in polishing experiments. The lever mechanism and Hoeckens connection enhance vibration parameters and motion efficiency, reducing surface flaws in SiC and improving uniformity. Adjusting the RVAPD structure and using the proposed method significantly improve SiC surface quality.
Stratification of Gut Microbiota Profiling Based on Autism Neuropsychological Assessments
Authors: Marangelo Chiara , Vernocchi Pamela , Del Chierico Federica , Scanu Matteo , Marsiglia Riccardo , Petrolo Emanuela , Fucà Elisa , Guerrera Silvia , Valeri Giovanni , Vicari Stefano , Putignani Lorenza
Published: October 2024

Autism spectrum disorder (ASD) is a neurodevelopmental disorder. Investigations of gut microbiota (GM) play an important role in deciphering disease severity and symptoms. Overall, we stratified 70 ASD patients by neuropsychological assessment, based on Calibrated Severity Scores (CSSs) of the Autism Diagnostic Observation Schedule-Second edition (ADOS-2), Child Behavior Checklist (CBCL) and intelligent quotient/developmental quotient (IQ/DQ) parameters. Hence, metataxonomy and PICRUSt-based KEGG predictions of fecal GM were assessed for each clinical subset. Here, 60% of ASD patients showed mild to moderate autism, while the remaining 40% showed severe symptoms; 23% showed no clinical symptoms, 21% had a risk of behavior problems and 56% had clinical symptoms based on the CBCL, which assesses internalizing problems; further, 52% had no clinical symptoms, 21% showed risk, and 26% had clinical symptoms classified by CBCL externalizing problems. Considering the total CBCL index, 34% showed no clinical symptoms, 13% showed risk, and 52% had clinical symptoms. Here, 70% of ASD patients showed cognitive impairment/developmental delay (CI/DD). The GM of ASDs with severe autism was characterized by an increase in Veillonella, a decrease in Monoglobus pectinilyticus and a higher microbial dysbiosis index (MDI) when compared to mild-moderate ASDs. Patients at risk for behavior problems and showing clinical symptoms were characterized by a GM with an increase of Clostridium, Eggerthella, Blautia, Intestinibacter, Coprococcus, Ruminococcus, Onthenecus and Bariatricus, respectively. Peptidoglycan biosynthesis and biofilm formation KEGGs characterized patients with clinical symptoms, while potential microbiota-activated PPAR-γ-signaling was seen in CI/DD patients. This evidence derived from GM profiling may be used to further improve ASD understanding, leasing to a better comprehension of the neurological phenotype.
Modulating Near-Infrared Persistent Luminescence via Diverse Preparation Approaches
Authors: Wang Xiaomeng , Zhu Hengli , Liu Yan , Li Jingyuan , Cao Lejia , Du Jiaren , Lin Hengwei
Published: October 2024

Near-infrared (NIR) persistent luminescence (PersL) materials have attracted extensive attention due to their great promise in medical diagnostics, bio-imaging, night vision surveillance, multi-level anticounterfeiting, and information encryption. To achieve NIR PersL (micro/nano-) materials with the desired properties, a variety of synthesis methods have been employed, including solid-phase reaction and liquid-phase synthesis. Different synthesis methods have different but important effects on the micro/nano-structure, luminescence, and PersL properties of the materials. Moreover, the influence of various synthesis methods on the properties of NIR PersL materials determines the selection of preparation approaches for other new material systems. Taking the representative NIR PersL ZnGa2O4:Cr3+ material as an example, four synthesis procedures are applied, namely, high-temperature solid-state reaction (SSR), high-temperature molten salt method (MSM), hydrothermal method (HM), and microwave-assisted solid-state (MASS) method. The structural and luminescent properties of samples made by SSR, MSM, HM, and MASS are compared. Notably, it is revealed that the MASS method can create additional trapping energy levels, which is of great significance for emerging applications. This work demonstrates the different effects of synthesis methods on PersL performance and provides a good guideline for the rapid and reasonable selection of preparation methods for diverse applications.
Vitamin D Levels in the United States: Temporal Trends (2011–2018) and Contemporary Associations with Sociodemographic Characteristics (2017–2018)
Authors: Subramanian Anita , Burrowes Hyacinth B. , Rumph Jelonia T. , Wilkerson Jesse , Jackson Chandra L. , Jukic Anne Marie Z.
Published: October 2024

Background: The most recent vitamin D data from the National Health and Nutrition Examination Survey (NHANES) have not been examined. We used data from NHANES to describe trends in 25-hydroxyvitamin D [25(OH)D] from 2011 to 2018 and for the most recent cycle (2017–2018) to identify groups with lower levels of 25(OH)D and factors predictive of 25(OH)D. Methods: The 31,628 participants were weighted to represent the entire U.S. population. For each 2-year NHANES survey cycle (2011 to 2018), we calculated the weighted median (25th and 75th percentiles) of 25(OH)D and the proportion of the population within the following categories (nmol/L): <30, 30-<50, 50-<75, 75-<125, and ≥125. For 2017–2018, we stratified by demographic and behavioral factors. Multivariate linear regression identified variables predictive of 25(OH)D. Results: The median 25(OH)D (nmol/L) increased slightly from 2013–2014 [66.5 (25th and 75th percentiles: 51.3, 83.0)] to 2017–2018 [68.7 (52.3, 87.8)], and the prevalence of 25(OH)D <50 nmol/L decreased slightly (23.4% vs. 21.3%). In 2017–2018, characteristics associated with lower 25(OH)D were age (12–39 years), male gender, non-Hispanic Black, higher BMI, lower income and education, winter season, not taking vitamin D supplements, or “never” using sunscreen. When stratified by age, race/ethnicity, and gender simultaneously, median 25(OH)D was lowest among non-Hispanic Black females aged 12–19 (38.5 nmol/L) or 20–39 (38.9 nmol/L). Predictors of 25(OH)D level differed by race/ethnicity, e.g., increasing BMI was associated with larger decrements in 25(OH)D among Mexican Americans. Conclusions: This analysis is the first to examine vitamin D levels stratified by multiple characteristics simultaneously. This strategy identified populations at higher risk for health sequelae due to low levels of vitamin D. For example, high levels of deficiency were found in non-Hispanic Black females of reproductive age.
Exploring Associations and Mediating Factors between Multiple Trace Metals with Anemia in US Adults: Insight from NHANES 2017–2020
Authors: Xie Lijie , Guan Xinchao , Zhou Yixiang , He Yujie , Chen Shilin , Xiao Wanting , Yang Jilong , Lu Jianyong , Hong Liecheng , Hu Qiansheng , Wang Qiong , Li Chuanwen , Wang Qing
Published: October 2024

Background: Anemia significantly contributes to the global disease burden, with its incidence potentially influenced by the trace metal content within the body. Objective: This study aims to examine the associations between trace metals and anemia risk, with a particular focus on investigating the potential mediating roles of iron status and inflammation in these associations. Methods: Five trace metals (Ni, Co, Mn, Se, and Mo) were examined in 1274 US adults, utilizing data from the National Health and Nutrition Examination Survey (NHANES) 2017–2020. The individual and combined effects of these metals on anemia were assessed using logistic regression, quantile g-computation (QGC), and Bayesian kernel machine regression (BKMR). A sex-stratified analysis was conducted to discern any gender-specific susceptibilities. Additionally, mediation analysis was employed to explore the potential mediating roles of iron status and inflammation in the associations between these metals and anemia. Results: Increased risks of anemia were positively associated with Co and Ni levels but negatively correlated with Se and Mn levels (all with p < 0.05). The trace metal mixture was negatively associated with anemia, with the highest weights of Co and Se in different directions in both the QGC and BKMR models. In the sex-specific analysis, we observed less pronounced protective effects from trace metals in females. Moreover, the mediating proportion of the iron status and inflammation in these relationships ranged from 10.29% to 58.18%. Conclusion: Our findings suggest that the trace element mixture was associated with decreased anemia risk, among which Se was a protective factor while Co was a risk factor, and females were more susceptible. The effects of these trace metals on anemia may be mediated by the iron status and inflammation.
Clinical Identification of Two Novel C. kroppenstedtii-like Species Isolated as Pathogens of Granulomatous Lobular Mastitis
Authors: Xiao Nan , Zhao Xiu-Ying
Published: October 2024

Granulomatous lobular mastitis (GLM) is a rare benign breast inflammatory disease that affects women of childbearing age. Corynebacterium species, especially Corynebacterium kroppenstedtii, was reported as the pathogen of GLM. A recent study showed that the C. kroppenstedtii complex is composed of C. kroppenstedtii and two novel species, C. parakroppenstedtii and C. pseudokroppenstedtii. The study presents seven C. kroppenstedtii-like strains isolated from GLM patients. However, they turned out to be six strains of C. parakroppenstedtii and one strain of C. pseudokroppenstedtii according to 16sRNA sequencing. In order to conduct a phylogenetic study, we further sequenced the fusA and rpoB genes, which were frequently employed in studies of Corynebacterium species. Novel Mass Spectral Peaks (MSPs) for C. parakroppenstedtii were created with Bruker MALDI-TOF MS. Then, the identification power of the MSPs was tested by C. parakroppenstedtii strains and remotely related Corynebacterum spp. The antibiotic sensitivity tests were performed according to the CLSI M45 guidelines. All of the strains were not resistant to β-lactams, vancomycin or linezolid. However, applying erythromycin and clindamycin could be fruitless. Phenotypic identification using a Vitek2 ANC ID card proved all of the C. parakroppenstedtii strains were identified as Actinomycete naeslundii. The test of Ala-Phe-Pro arylamidase and urease could be employed as the characteristics to distinguish C. pseudokroppenstedtii from C. parakroppenstedtii. Here, we present the identification, antibiotic sensitivity tests (ASTs) and epidemiological investigation of two novel C. kroppenstedtii-like species with the purpose of improving the understanding of C. kroppenstedtii-like species and related diseases.
Molecular and Morphological Characteristics of a Novel Cyst Nematode in the Rhizosphere of Artemisia lavandulaefolia DC. in Gansu Province, Northwest China
Authors: Guo Wei , Li Huixia , Wei Xuejuan , Luo Ning , Shi Mingming
Published: October 2024

Cyst nematodes are obligate parasitic nematodes found in the fields of many cultivated crops. These nematodes, which have great economic importance, pose a threat to food security, though they are frequently ignored or misdiagnosed as pests because of covert parasitism. A cyst nematode population parasitizing on Artemisia lavandulaefolia DC., one of the traditional Chinese medicines was collected in Gansu Province. The species was diagnosed using integrative taxonomy and molecular approaches. The cyst population is spherical or lemon-shaped, light brown or dark brown in color, with a long neck and a protruding vulval cone. The stylet of the second-stage juvenile is strong, and the front end of the ball at the base of the stylet is concave; the median bulb and excretory pore are prominent; the tail is blunt and circular, and the transparent tail is usually shorter than the stylet. A phylogenetic analysis was carried out using the internal transcribed spacer (ITS) and 28S genes of ribosomal DNA, which further confirmed the presence of Cactodera chenopodiae. According to our literature review, this is the first report on C. chenopodiae in Compositae. By following this research, we can better understand the challenges posed by A. lavandulaefolia DC. and develop effective strategies for managing its spread and impacts. This will help to protect vulnerable ecosystems and ensure the sustainability of agricultural and forestry activities in affected areas.
Potential Anti-Obesity Effect of Hazel Leaf Extract in Mice and Network Pharmacology of Selected Polyphenols
Authors: Zhao Jiarui , Alimu Aikebaier , Li Yvmo , Lin Zhi , Li Jun , Wang Xinhe , Wang Yuchen , Lv Guangfu , Lin He , Lin Zhe
Published: October 2024

Background: Obesity is gradually becoming a widespread health problem, and treatment using natural compounds has seen an increasing trend. As a by-product of hazelnut, hazel leaf is usually disposed of as waste, but it is widely used in traditional and folk medicines around the world. Aim of this study: Based on previous studies, the effects of the regulation of lipid metabolism and the mechanism of hazel leaf polyphenol extraction obesity were investigated. Methods: In this study, a high-fat diet-fed mouse model of obesity and 3T3-L1 preadipocytes were established. The ameliorative effects of the hazel leaf polyphenol extract on obesity and the regulating lipid metabolisms were explored based on network pharmacology, gut microbiota, and molecular docking. Results: Network pharmacology showed that hazel leaf polyphenols may play a role by targeting key targets, including PPARγ, and regulating the PPAR signaling pathway. They significantly improved body weight gain, the liver index, and adiposity and lipid levels; regulated the gut microbiota and short-chain fatty acid contents; down-regulated the expression of lipid synthesis proteins SREBP1c, PPARγ, and C/EBP-α; and up-regulated the expression of p-AMPK in obese mice. They inhibited the differentiation of 3T3-L1 cells, and the expression of related proteins is consistent with the results in vivo. The molecular docking results indicated that gallic acid, quercetin-3-O-beta-D-glucopyranoside, quercetin, myricetin, and luteolin-7-O-glucoside in the hazel leaf polyphenol extract had strong binding activities with PPARγ, C/EBP-α, and AMPK. Conclusions: The results demonstrate that the hazel leaf polyphenol extract can improve obesity by regulating lipid metabolism, which provides a valuable basis for developing health products made from hazel leaf polyphenols in the future.
Provinols™, a Polyphenolic Extract of Red Wine, Inhibits In-Stent Neointimal Growth in Cholesterol-Fed Rabbit
Authors: Elbaz Meyer , Roul Gérald , Andriantsitohaina Ramaroson
Published: October 2024

Background/Objectives: Epidemiological studies indicate a potential correlation between the consumption of polyphenols and a reduced risk of developing cardiovascular disorders. The present study investigates the potential of a red wine polyphenol oral extract, Provinols™, to reduce neointimal hyperplasia following angioplasty in a hypercholesterolemic rabbit model. Methods: New Zealand white rabbits were fed 1% cholesterol-enriched chow for a period of eight weeks prior to the induction of iliac balloon injury and subsequent stent placement. Following the implantation of the stent, Provinols™ (20 mg/kg/day) or an identical placebo was administered orally for a period of four weeks in a randomized manner. Twenty-eight days following the stenting procedure, the arteries were harvested after euthanasia and subjected to histology assignment analysis. Results: The administration of Provinols™ did not result in a statistically significant change in either blood pressure or plasma cholesterol levels. However, Provinols™ treatment led to a notable reduction in the growth of the intima within the stented area, as well as a reduction in the thickness and surface area of the intima. It is of note that treatment with Provinols™ was associated with a reduction in the accumulation of fat within the arteries and a diminished inflammatory response to injury. Conclusions: The findings demonstrate that oral administration of Provinols™ has the potential to reduce in-stent neointimal growth and lipid deposition, likely due to its anti-inflammatory properties in iliac arteries from hypercholesterolemic rabbits. Additionally, these findings provide an evidence-based rationale for the potential therapeutic benefits of plant-derived polyphenols in the prevention of restenosis associated with stent placement.
Effect of Gas Exchange Rate, Vessel Type, Planting Density, and Genotype on Growth, Photosynthetic Activity, and Ion Uptake of In Vitro Potato Plants
Authors: Vollmer Rainer , Espirilla Janeth , Espinoza Ana , Villagaray Rosalva , Castro Mario , Pineda Sandra , Sánchez Juan Carlos , Mello Alexandre F. S. , Azevedo Vania C. R.
Published: October 2024

The growth of high-quality in vitro potato plants (Solanum stenotomum subsp. stenotomum, Solanum stenotomum subsp. goniocalyx, and Solanum tuberosum subsp. andigena) is affected by multiple biological, operational, and environmental factors. Research on in vitro culture is frequently focused on the species, explant, composition of the culture medium, and incubation conditions, but only limited information is available on the effect of the gas exchange rate and volume of in vitro culture vessels under variable planting densities. In the present study, these factors were evaluated with a set of seven diverse potato landraces. The results were compared to the plants’ responses in routinely used in vitro culture vessels, i.e., 13 × 100 mm and 25 × 150 mm test tubes, and GA7® magenta vessels. In vitro potato plants grown in plastic vessels equipped with a HEPA filter delivering a high gas exchange rate developed thicker stems (0.95 mm), a higher total average leaf area (2.51 cm2), increased chlorophyll content in leaves (32.2 ppm), and lower moisture content in their tissues (90.1%) compared to filter systems with lower gas exchange rates. A high planting density of 10 × 10 plants per vessel (360 and 870 mL) negatively affected the average stem width and root length but increased the plant height (3.4 cm). High fluctuations of ion-uptake of NO3−, Ca++, K+, and Na+ were observed between genotypes, with some accessions having a 4.6-times higher Ca++-ion concentration in their tissues (190–234 ppm). The in vitro plants developed more robust stems, longer roots, and larger leaves within in vitro culture vessels equipped with a HEPA filter (high gas exchange rate) compared to the control vessels, in contrast to the chlorophyll content in leaves, which was higher in plants grown in narrow test tubes. Depending on the purpose of the subculture of in vitro plants, their growth and development can be molded using different gas exchange rates, planting densities, and vessel volumes.
Comparative Insights into Photosynthetic, Biochemical, and Ultrastructural Mechanisms in Hibiscus and Pelargonium Plants
Authors: Falcioni Renan , Antunes Werner Camargos , de Oliveira Roney Berti , Chicati Marcelo Luiz , Demattê José Alexandre M. , Nanni Marcos Rafael
Published: October 2024

Understanding photosynthetic mechanisms in different plant species is crucial for advancing agricultural productivity and ecological restoration. This study presents a detailed physiological and ultrastructural comparison of photosynthetic mechanisms between Hibiscus (Hibiscus rosa-sinensis L.) and Pelargonium (Pelargonium zonale (L.) L’Hér. Ex Aiton) plants. The data collection encompassed daily photosynthetic profiles, responses to light and CO2, leaf optical properties, fluorescence data (OJIP transients), biochemical analyses, and anatomical observations. The findings reveal distinct morphological, optical, and biochemical adaptations between the two species. These adaptations were associated with differences in photochemical (AMAX, E, Ci, iWUE, and α) and carboxylative parameters (VCMAX, ΓCO2, gs, gm, Cc, and AJMAX), along with variations in fluorescence and concentrations of chlorophylls and carotenoids. Such factors modulate the efficiency of photosynthesis. Energy dissipation mechanisms, including thermal and fluorescence pathways (ΦPSII, ETR, NPQ), and JIP test-derived metrics highlighted differences in electron transport, particularly between PSII and PSI. At the ultrastructural level, Hibiscus exhibited optimised cellular and chloroplast architecture, characterised by increased chloroplast density and robust grana structures. In contrast, Pelargonium displayed suboptimal photosynthetic parameters, possibly due to reduced thylakoid counts and a higher proportion of mitochondria. In conclusion, while Hibiscus appears primed for efficient photosynthesis and energy storage, Pelargonium may prioritise alternative cellular functions, engaging in a metabolic trade-off.