bluuebunny/crossref_metadata_2025 huggingface dataset:
The essence and features of the legal regulation of budget expenditures on the sector of national security and defense under the conditions of the state of martial
Authors: Shpak I.
Published: December 2023

The article deals with the essence and peculiarities of the legal regulation of budget expenditures for the national security and defense sector in the conditions of martial law, and determines their impact on ensuring the country's economy, defense capability, as well as the security of the state and society. It is substantiated that ensuring the appropriate level of financing of the national security and defense sector is an important component for the effective functioning and protection of the state. It was found that the source of resources for the implementation of such priority functions of the state as ensuring national security and defense are the funds of the state budget and local budgets. The actual definition of the concept of «budget expenditures» was formulated, and the analysis of the main legal acts regulating the financing of the security and defense sector of Ukraine was carried out. The peculiarities of financing and logistical support of the national resistance in particular, and the national security and defense sector as a whole are highlighted. It is pointed out the need to make changes to regulatory legal acts to ensure proper financing of the national security and defense sector, taking into account the requirements for prevention and elimination of factors that contribute to the emergence and spread of corruption in this area. Proposals have been formulated to improve the current legislation of Ukraine in terms of ensuring proper financing of the national security and defense sector and effective use of budget expenditures for the specified sector in war conditions, in particular: increasing expenditures for the national security and defense sector and determining their optimal structure; filling local budgets due to effective actions of local authorities; development of a unified budget strategy for financing the security and defense sector; ensuring proper state control over budget expenditures for the security and defense sector by creating a separate independent unit.
Mobilization Economy and the Fastest Learning Possible
Authors:   Independent researcher, Abolmasov Alexey V. , Dolzhenko Ruslan A. ,   Ural State University of Economics
Published: December 2023

The current situation in the world and the economy, as well as the goals facing the country, require a rapid in-crease in production efficiency, building new supply chains, and developing new types of activities. Traditional approaches to training and development of specialists, relevant for the “slow” existence of business, are not sufficient to accelerate all processes to achieve set goals. For the current time, new approaches to fast and ef-fective learning are needed, which can be summarized as a methodology for maximally rapid learning (MRL). The aim of the research is to study different methodologies and tools of fast and effective learning, generaliza-tion of which will make it possible to form the author’s methodology for the fastest learning possible. Document analysis and generalization methods were used in this work. The results of the research made it possible to formulate a basic understanding of the methodology for maximally rapid learning, associated with a set of measures and tools for the implementation of educational tasks.
Corporate Project Management System in Higher Education Organizations
Authors:   Omsk State Agrarian University named after P.A. Stolypin, Kryukova Olga N.
Published: December 2023

The article considers the practice of building a corporate project management system in educational organiza-tions of higher education; evaluates the effectiveness and quality of the processes of improving the corporate project management system; substantiates practical recommendations for the creation, implementation and improvement of the corporate project management system in the university of agrarian orientation. The rele-vance of the development of corporate project management systems of higher education organizations is justi-fied by the need for a systematic idea of project management, since the activities of universities are trans-formed towards the introduction of an open project model of student education. The aim of the study is to de-velop the regulatory and methodological foundations of the concept and practical recommendations for the creation, implementation and improvement of corporate project management system in the agrarian university to improve the satisfaction of project activity participants and increase the number of effectively implemented projects.
The Role of Project Practice in the Education of Young Engineering Specialists
Authors:   Samara State Technical University, Smirnova Svetlana B.
Published: December 2023

The rapid development of industrial enterprises in the Russian Federation, the progressive increase in capacity, and the opening of new production facilities have especially highlighted the existing problem with the training of engineering personnel. The number of university graduates trained in engineering and technical specialties and areas of training does not correspond to the required number of young specialists at enterprises. The exist-ing project practice of attracting young specialists – university graduates to industrial and construction enter-prises was formed in the last 10 years and did not take into account the situation of a large-scale shortage of qualified personnel. The research of many scientists is focused on analyzing the interaction between universi-ties and industrial enterprises in terms of the commercialization of scientific research, but the issue of as-sessing the effectiveness of project management to attract young specialists to industrial enterprises remains insufficiently studied.
Legal Status of Persons Released from Places of Deprivation of Liberty: Problems of Regulation
Authors:   Academy of the Federal Penitentiary Service of Russia, Golovastova Yulia A. ,   Moscow University named after S. Witte, Nistratova Irina S. ,   Academy of the Federal Penitentiary Service of Russia
Published: December 2023

The article analyzes the legal status of persons released from places of deprivation of liberty. The authors as-sessed this concept, defined its essence and content, noted the ambiguity of its interpretation and the need for differentiation with such concepts as “persons who have served deprivation of liberty”, “persons released from places of deprivation of liberty”, “persons released from serving deprivation of liberty”. Attention is also focused on the restrictions faced by these persons. It is emphasized that as part of the positive legislative changes, in particular, as a result of the formation of the institute of probation, it is necessary to develop a clear state-legal mechanism for the protection of the legal status of persons released from places of deprivation of liberty, with the aim of their employment, housing, restoration and formation of family and communicative ties, obtaining the necessary level of education, medical care, social services and other assistance.
About the Concept of “Trace of a Crime”
Authors:   National Research Lobachevsky State University of Nizhny Novgorod, Lushin Evgeny A.
Published: December 2023

The concept of a crime trace is one of the fundamental concepts of criminology. From a forensic point of view, the disclosure and investigation of a crime is the process of detecting, fixing the investigation of traces of crimi-nal activity and, based on the information received, detecting objects that have left these traces. Theoretical works concerning the concept of a trace, types of traces and patterns of work with traces in the investigation of crimes in our country appeared in the first half of the twentieth century. For a long time, most Soviet and Rus-sian criminologists understood the material traces of a crime as objects of the material world that objectively exist independently of the subject of the investigation. But there was another point of view. In accordance with it, the trace of the crime was considered as an objectively existing change in the environment reflected in the con-sciousness of the subject of proof. We generally agree with this point of view. However, it seems to the author necessary to make an addition: before being reflected in the consciousness of the subject of proof, the sur-rounding change resulting from the crime undergoes certain changes, including in the process of the interac-tion with the subject of investigation.
Imagen de Japón en los comics de México
Authors: Quartucci Guillermo
Published: December 2023

-
Wang Meng y la nueva narrativa china
Authors: Botton Beja Flora
Published: December 2023

-
Lina, Fruzzetti, The Gift ofa Virgin, Women, Marriage, and Ritual in a Bengali Society, New Brunswick, Rutgers University Press, 1982, 178 pp.
Authors: González del Solar Susana
Published: December 2023

-
Frank Joseph Shulman (comp. & ed.), Doctoral Dissertations on Japan and on Korea, 1969-1979: An Annotated Bibliography of Studies in Western Languages, Seattle and London, University of Washington Press, 1982, XV + 473 pp.
Authors: Quartucci Guillermo
Published: December 2023

-
Nacionalismo y arte en la India contemporánea
Authors: Chen-Apuy Espinosa Hilda
Published: December 2023

-
Ronald A. Morse, editor. The limits of Reform in China. Boulder, Colorado Westview Press, 1983. XII, 155 pp.
Authors: Cornejo Bustamante Romer
Published: December 2023

-
Cine asiático
Authors: Quartucci Guillermo
Published: December 2023

-
La importancia de los puntos cardinales en le ritual doméstico védico (según el Gobhila Gṛhya Sutra)
Authors: González Reimann Luis
Published: December 2023

-
La República Popular China y su incidencia en el mundo
Authors: Anguiano Roch Eugenio
Published: December 2023

-
Bangkok: la exclusión de los pobres
Authors: Durand-Lasserve Alain
Published: December 2023

-
Conferencia. El trasfondo cultural de la modernización japonesa, dictada por el Doctor Kadzuo Terada, de la Universidad de Tokio, en el Castillo de Chapultepec el 11 de Octubre de 1984
Authors: - -
Published: December 2023

-
Las restricciones principales que afectan el proceso de la industrialización en la India
Authors: Ahluwalia Isher Judge
Published: December 2023

-
Walter Gardini. El cristianismo en Corea, historia y actualidad. Obras misionales Pontificias, Ed. Guadalupe, Buenos Aires, 1984. 195 pp. (Colección Misión)
Authors: Ota Mishima María Elena
Published: December 2023

-
Silvia Betancourt Quesada, Problemas económicos en África Subsahariana, La Habana, Universidad de La Habana, 1984
Authors: Boudroua Ahmed
Published: December 2023

Traducción del inglés: Mariela Álvarez.
Agustín Jacinto Z., Zen y personalidad, El Colegio de Michoacán, Zamora, Mich., 1984
Authors: García Pío
Published: December 2023

-
Entre México y Japón. Los habitantes del "pedregal"
Authors: Tetsuroo Nakaoka
Published: December 2023

Traducción del japonés: Masayo Nonaka y Guillermo Quartucci.
Huang Hsiao, Katharine H.Y., Money and Banking in the Chinese Mainland, Taipei, Chung-Hua Institution for Economic Research, 1984, VIII + 98 pp. (Mainland China Economic Series núm. 1).
Authors: Cornejo Bustamante Romer
Published: December 2023

-
La trabajadora industrial en el sudeste de Asia
Authors: Choi Wan Cheung
Published: December 2023

-
Nuevas perspectivas para la literatura china
Authors: Cornejo Bustamante Romer
Published: December 2023

-
Afganistán: encrucijada estratégica del Asia Central
Authors: Quintana Pali Santiago
Published: December 2023

-
China y Japón en la cultura de masas de Asia
Authors: Quartucci Guillermo
Published: December 2023

-
Las tribulaciones del hermano Jero
Authors: Soyinka Wole
Published: December 2023

Traducción del ingés: Carmen Chuaqui.
Presentación
Authors: Zoctizoum Yarisse
Published: December 2023

Traducción del fránces: Laura González Durán.
Sobre los límites de la reforma política en China
Authors: Cornejo Bustamante Romer
Published: December 2023

-
Kenichi Ohmae, Beyond National Borders. Reflections on Japan and the World, Dow Jones-Irwin, Homewood, Illinois, 1987, 128 pp.
Authors: Hodara Joseph
Published: December 2023

-
Procesos migratorios y género en el cine sudamericano
Authors: Corradi Matias
Published: December 2023

Este trabajo busca indagar en torno de los procesos migratorios y sus mediaciones audiovisuales. Para ello, se abordan dos casos fílmicos que exponen el tema poniendo en relación, al interior del sistema de personajes, aspectos étnicos, lingüísticos, religiosos, sexuales, de género y de clase. El enfoque teórico metodológico utilizado articula el análisis espectacular y textual de ambas producciones con categorías provenientes de la antropología de las migraciones para pensar de manera comparada sobre el modo en que estas películas figuran a sus protagonistas migrantes, todas ellas mujeres.
Neoliberalismo y desigualdad social.
Authors: Dovio Mariana Angela
Published: December 2023

El neoliberalismo como racionalidad política remite a prácticas, dispositivos de poder y discursos que plantean reformas pro mercado y la subsidiariedad del Estado. Desde esta racionalidad, se aceleran procesos de mercantilización y exclusión social en diversos ámbitos de la vida cotidiana, entre ellos, el espacio público. En este trabajo estudiamos desde una racionalidad neoliberal, actividades de subsistencia en espacios públicos de la ciudad de Neuquén entre 2012 y 2021. Desde los diarios La Mañana y Rio Negro, de amplio alcance en la zona nor- patagónica, se abordaron efectos de la desigualdad social y usos mercantilizados del espacio público en Neuquén. En especial, describimos cómo se controló la actividad de quienes lavaban y cuidaban autos en el centro de la ciudad, a través de discursos de exclusión, en el momento de la implementación de un sistema de estacionamiento medido.
Encuadres en la cobertura sobre IA en Argentina
Authors: Azzara Esteban
Published: December 2023

Este trabajo se propone dar cuenta del modo en que los medios digitales más populares de Argentina presentaron, durante la primera mitad de 2023, el tema "Inteligencia Artificial".

Con ese propósito construimos un corpus de datos integrado por cien noticias de los cinco medios digitales más populares de Argentina (Infobae, TN online, Clarín online, La Nación online, A24 online) que hayan sido consideradas en cada uno de los medios como “más relevante” en junio de 2023.

El análisis de este corpus nos permitió identificar que los principales medios digitales de Argentina, presentaron la información sobre Inteligencia Artificial a partir de cuatro encuadres: Impacto sobre actividades laborales y/o productivas, Elaboración y consumo de contenidos para redes sociales y ocio, Consecuencias a futuro, Regulaciones y límites.

En la sección de resultados de este trabajo ofrecemos dos tablas en las que presentamos el total de las noticias distribuidas según sus tópicos y temas y la información de la frecuencia con que cada uno de los encuadres fue identificado en el corpus de noticias estudiado, señalando su valoración positiva o negativa.
Inteligencia Artificial Generativa y la Formación de Formadores
Authors: Rosignoli Silvina
Published: December 2023

Este artículo representa un avance inicial de una tesis de maestría en Tecnología Educativa en curso, centrada en la formación de formadores para la integración de la Inteligencia Artificial Generativa (IAG) en la enseñanza universitaria. Se aborda el desarrollo de un marco teórico en construcción y se da cuenta de las indagaciones preliminares a  docentes universitarios y expertos en el campo, resaltando los desafíos didácticos que la integración de estas tecnologías presentan para la enseñanza. Se subraya la necesidad de una reflexión crítica y propositiva sobre la inclusión de estas tecnologías en la enseñanza universitaria. Se dejan abiertos algunos interrogantes.
¡Abajo el tirano de Amaya!: un motín partidario en la Penitenciaría de Córdoba (1908-1916)
Authors: Luciano Milena
Published: December 2023

La incorporación de las provincias al reformismo penitenciario nacional de finales del siglo XIX no fue una tarea sencilla. En Córdoba la dimensión presupuestaria ocupó un lugar preponderante que demoró la configuración de un área burocrática específica abocada a la administración de la Penitenciaría. En ese marco, las innovaciones producidas en los primeros años de vida del penal se encuentran asociadas a la gestión específica del director Antonio Amaya (1908-1916), funcionario que debió renunciar luego de la sublevación de internos producida el 18 de mayo de 1916. Como demostramos en este artículo, este acontecimiento puso en evidencia la relevancia que tuvieron las luchas provinciales y nacionales para explicar tanto el nombramiento de Amaya como su destitución. A través del análisis de las crónicas de la prensa local y de informes y memorias institucionales, desglosamos las diferentes causas del motín, las demandas de los internos y las conexiones con los cambios políticos producidos con la llegada del radicalismo a la presidencia de la Nación. De este modo, situamos al funcionario en el universo de disputas partidarias, más allá de su voluntad reformadora.
Contrato de obra intelectual y derechos de autor
Authors: Leturia Mauro Fernando , Gochicoa Adrián Emir
Published: December 2023

El presente texto tiene como finalidad el análisis al régimen legal del contrato de obra, específicamente referido a las obras intelectuales. Desde este aspecto, se hará especial referencia a la protección de derechos de autor acorde a la Ley 11.723 y su influencia en el contrato de obra regulado por el Código Civil y Comercial de la Nación, haciendo hincapié en el caso de las obras arquitectónicas.
The road to carbon trading in Malaysia: Unpacking knowledge and experience through scoping review and in-depth interviews
Authors: Alias Amran , Saarani Asmawi Noor , Amran Azlan , Abbasi Munir A. , Ghobakhloo Morteza
Published: December 2023

The emission trading scheme (ETS) is arguably one of the most effective approaches for encouraging industries to transition to a low-carbon economy and, as a result, assisting nations in meeting their goals under the United Nations Framework Convention on Climate Change to mitigate the challenge of climate change. ETS is gaining popularity as more governments throughout the world contemplate implementing it, particularly in developing countries. Much of the existing research has concentrated on debates concerning ETS operations in developed nations. This study is to give a discourse of the success criteria for ETS implementation that have been identified in the literature and then cross-referenced in the context of Malaysia. For this, the research used an integrated approach of scoping review of existing literature and in-depth interviews with Malaysian stakeholders. Using Narassimhan et al. (2018)’s ETS assessment framework, the scoping review identified five major attributes that lead to successful ETS implementation in a global context that are environmental effectiveness, economic efficiency, market management, stakeholder engagement, and revenue management. In-depth interviews with several groups of discovered stakeholder engagement as an essential attribute that would play a critical role in advancing ETS implementation in Malaysia. The study concludes by proposing a complete strategy based on empirical information and first-hand narratives, providing useful insights for politicians, industry players, and environmental activists. The recommendation is especially important as Malaysia strives to improve its commitment to sustainable and responsible development in light of the challenges posed by climate change.
Unveiling the connection among customer concentration, trade credit financing, and firm performance: A moderated mediation model
Authors: Qiang Meng , Dampitakse Kusuma , Tancho Nartraphee
Published: December 2023

The augmentation of firm performance via customer concentration is particularly indispensable for organizational evolution. Both trade credit financing and financing constraints play pivotal roles in the nexus between customer concentration and performance. This research constructs a moderated mediation model to rigorously investigate the impact of customer concentration on firm performance, positing trade credit financing as the mediating variable and financing constraints as the moderating variable. The relevant hypotheses are evaluated empirically using panel data compiled from listed manufacturing firms in China over the period 2013–2020, yielding 8 firm-year observations. The empirical outcomes denote that customer concentration exerts a positive influence on firm performance, albeit having a negative impact on trade credit financing. Trade credit financing serves as a partial mediator in the relationship between customer concentration and manufacturing firm performance. Financing constraints are found to positively moderate the mediating role of trade credit financing in the relationship between customer concentration and firm performance. This research broadens the understanding of the implications of customer relationships on trade credit financing and performance, thereby enriching the knowledge base for managing a firm’s financing channels more effectively.
Clinical and prognostic implications of heart failure hospitalization in patients with advanced heart failure
Authors: Pagnesi Matteo , Sammartino Antonio Maria , Chiarito Mauro , Stolfo Davide , Baldetti Luca , Adamo Marianna , Maggi Giuseppe , Inciardi Riccardo Maria , Tomasoni Daniela , Loiacono Ferdinando , Maccallini Marta , Villaschi Alessandro , Gasparini Gaia , Montella Marco , Contessi Stefano , Cocianni Daniele , Perotto Maria , Barone Giuseppe , Merlo Marco , Cappelletti Alberto Maria , Sinagra Gianfranco , Pini Daniela , Metra Marco , Lombardi Carlo Mario
Published: December 2023

Background

Hospitalization is associated with poor outcomes in patients with heart failure, but its prognostic role in advanced heart failure is still unsettled. We evaluated the prognostic role of heart failure hospitalization in patients with advanced heart failure.

Methods

The multicenter HELP-HF registry enrolled consecutive patients with heart failure and at least one high-risk ‘I NEED HELP’ marker. Characteristics and outcomes were compared between patients who were hospitalized for decompensated heart failure (inpatients) or not (outpatients) at the time of enrolment. The primary endpoint was the composite of all-cause mortality or first heart failure hospitalization.

Results

Among the 1149 patients included [mean age 75.1 ± 11.5 years, 67.3% men, median left ventricular ejection fraction (LVEF) 35% (IQR 25–50%)], 777 (67.6%) were inpatients at the time of enrolment. As compared with outpatients, inpatients had lower LVEF, higher natriuretic peptides and a worse clinical profile. The 1-year rate of the primary endpoint was 50.9% in inpatients versus 36.8% in outpatients [crude hazard ratio 1.70, 95% confidence interval (CI) 1.39–2.07, P < 0.001]. At multivariable analysis, inpatient status was independently associated with a higher risk of the primary endpoint (adjusted hazard ratio 1.54, 95% CI 1.23–1.93, P < 0.001). Among inpatients, the independent predictors of the primary endpoint were older age, lower SBP, heart failure association criteria for advanced heart failure and glomerular filtration rate 30 ml/min/1.73 m2 or less.

Conclusion

Hospitalization for heart failure in patients with at least one high-risk ‘I NEED HELP’ marker is associated with an extremely poor prognosis supporting the need for specific interventions, such as mechanical circulatory support or heart transplantation.
Next-generation sequencing-based liquid biopsy may be used for detection of residual disease and cancer recurrence monitoring in dogs
Authors: McCleary-Wheeler Angela L. , Fiaux Patrick C. , Flesner Brian K. , Ruiz-Perez Carlos A. , McLennan Lisa M. , Tynan John A. , Hicks Susan C. , Rafalko Jill M. , Grosu Daniel S. , Chibuk Jason , O'Kell Allison L. , Cohen Todd A. , Chorny Ilya , Tsui Dana W.Y. , Kruglyak Kristina M. , Flory Andi
Published: December 2023

OBJECTIVE

The purpose of this study was to evaluate the performance of a next-generation sequencing-based liquid biopsy test for cancer monitoring in dogs.

SAMPLES

Pre- and postoperative blood samples were collected from dogs with confirmed cancer diagnoses originally enrolled in the CANcer Detection in Dogs (CANDiD) study. A subset of dogs also had longitudinal blood samples collected for recurrence monitoring.

METHODS

All cancer-diagnosed patients had a preoperative blood sample in which a cancer signal was detected and had at least 1 postoperative sample collected. Clinical data were used to assign a clinical disease status for each follow-up visit.

RESULTS

Following excisional surgery, in the absence of clinical residual disease at the postoperative visit, patients with Cancer Signal Detected results at that visit were 1.94 times as likely (95% CI, 1.21 to 3.12; P = .013) to have clinical recurrence within 6 months compared to patients with Cancer Signal Not Detected results. In the subset of patients with longitudinal liquid biopsy samples that had clinical recurrence documented during the study period, 82% (9/11; 95% CI, 48% to 97%) had Cancer Signal Detected in blood prior to or concomitant with clinical recurrence; in the 6 patients where molecular recurrence was detected prior to clinical recurrence, the median lead time was 168 days (range, 47 to 238).

CLINICAL RELEVANCE

Next-generation sequencing-based liquid biopsy is a noninvasive tool that may offer utility as an adjunct to current standard-of-care clinical assessment for cancer monitoring; further studies are needed to confirm diagnostic accuracy in a larger population.
Exposure to emulsified isoflurane and sevoflurane protects canine primary hepatocytes against hypoxia-induced apoptosis
Authors: Obeid Patricia de Carvalho Ibrahim , Natalini Claudio Correa , Howell George Eli
Published: December 2023

OBJECTIVE

Emulsified isoflurane and sevoflurane have immunomodulating and anti-inflammatory effects in vital organs such as the brain, myocardium, and kidneys subjected to ischemia-reperfusion injury. This study aims to investigate the cellular protective effects of both emulsified anesthetics in cultured canine hepatocytes.

PROCEDURES

We analyzed the apoptosis and viability responses of cultured primary canine hepatocytes exposed to 1% O2 for 30 versus 120 minutes after being treated with emulsified isoflurane or sevoflurane in 10% lipid, or 10% lipid alone or no-treatment control at 24 hours of reoxygenation (21% O2).

RESULTS

After 120 minutes of hypoxia, the hepatocytes that received either emulsified isoflurane or sevoflurane treatments had significantly decreased apoptosis at 24 hours of reoxygenation in comparison to the 10% lipid treatment. Also, the no-treatment control group had significantly higher apoptosis at 24 hours of reoxygenation when exposed to 120 minutes of hypoxia compared to 30 minutes of hypoxia. Neither 30 nor 120 minutes of hypoxia or exposure to 10% lipid, emulsified isoflurane, or emulsified sevoflurane altered overall cellular viability at 24 hours of reoxygenation.

CLINICAL RELEVANCE

This study demonstrated that both isoflurane and sevoflurane, in the emulsified form, have the potential to reduce the apoptotic response of cells under oxygen deprivation. Therefore, this attribute of both halogenated anesthetics suggests an alternative treatment to be applied in live patients submitted to surgical stabilization of organs and tissues under the risk of ischemia and reperfusion injury.
Predicting an increased risk of severe clinical mastitis and economic loss using a threshold value of bovine leukemia virus proviral load
Authors: Watanabe Aiko , Murakami Hironobu , Kakinuma Seiichi , Murao Koki , Oomae Kaori , Akamatsu Hirohisa , Seto Takahiro , Shinozuka Yasunori , Kawai Kazuhiro
Published: December 2023

OBJECTIVE

To establish a threshold value of bovine leukemia virus (BLV) proviral load (PVL) to identify increased risk of severe clinical mastitis, and to examine the prognosis and economic loss of clinical mastitis based on the newly established PVL cut-off value.

ANIMALS

97 lactating Holstein cows with clinical mastitis.

METHODS

Blood and milk samples were collected aseptically from each cow. Youden index was used for receiver-operating characteristic curve analysis with the severity rate of clinical mastitis as the dependent variable and PVL as an independent variable. PVL cut-off value was used as a criterion to compare the severity rate of clinical mastitis, percentage of cows with and without systemic treatments, number of treatments, cost of treatment, and prognosis.

RESULTS

PVL cut-off value was 17.8 copies/10 ng DNA for the dependent variable MILD vs SEVERE. The severity rate of clinical mastitis, percentage of cows given systemic treatments, and technical fees for medical treatment were significantly higher in the group above the PVL cut-off value than in the group below the PVL cut-off value and the negative group. Number of treatments was significantly higher in the group above the cut-off value than in the group below the cut-off value. There was no significant difference in prognosis after mastitis among the 3 groups.

CLINICAL RELEVANCE

These results suggested that PVL cut-off value of 17.8 copies/10 ng DNA was a useful threshold for increased economic losses in BLV-infected cows; it may also serve as a new standard value for the detection and culling of BLV-infected cows in Japan.
Repair of Cracks in Concrete with the Microbial-Induced Calcite Precipitation (MICP) Method
Authors: Özhan Hacer Bilir , Yildirim Musa , Öğüt Hamdi , Öz Hilal Girgin
Published: December 2023

In this study, the microbiologically-induced calcium carbonate precipitation (MICP) method was employed to examine its potential for repairing cracks in concrete. In addition, specific gravity and porosity values were measured to examine the effect of calcite formations on concrete surfaces and microstructures. Bacteria-supplemented concrete repaired cracks up to 0.4 mm wide by filling them with CaCO3. Furthermore, this study not only examined the healing of the width but also the length of cracks. However, as the width of the treated cracks decreased, their length increased. This indicated that the MICP treatment is more effective in a limited crack range. Specific gravity values increased, and porosity values decreased in concrete supplemented with calcifying bacteria. SEM analyses showed that calcite is a bacterial product that forms a very tight bond with a cement gel and that calcite fills visible cracks and voids and creates more of a void-free and undamaged concrete structure.
Behavior of an Embankment on Stone Column-Reinforced Soft Soil
Authors: Wassie Tadesse Abebe , Demir Gökhan
Published: December 2023

Reinforcing soft soils with good drainage and load-bearing material is a common practice in the construction of embankments. Stone columns are among the granular materials that accelerate consolidation settlements due to their high drainage capacities. This study aims to investigate the behavior of an embankment on stone column-reinforced soft soil. The embankment was analyzed using the Plaxis 2D Finite Element Method. The columns were converted into equivalent walls using the plane strain conversion method. This method was validated with field measurement results from previous studies and showed good agreement. According to the research findings, stone columns significantly reduced deformations and improved consolidation. For instance, the ultimate settlement was decreased from 54.3 mm to 33.1 mm for 10% and 20% area replacement ratios, respectively. As the safety analysis verified, basal geosynthetics contributed substantially to the embankment’s stability. Furthermore, the safety factor values increased for all stages of the construction, and the global safety factor increased at the end of the construction. Stone columns and basal geogrid-reinforced soft soil can reduce settlement and increase the stability of an embankment.
Effect of Recovered Fibers on the Behavior of Concrete in Tension
Authors: Atlaoui Djamal , Adjrad Arezki , Bouafia Youcef
Published: December 2023

This study evaluates the performance of concrete under the effect of the addition of non-straight fibers recovered during the machining of steel parts and then studies the effect of the addition of corrugated fibers (chips) on their behavior in direct traction of the fiber concrete. In order to characterize the mechanical behavior and tearing of these fibers, three lengths of fibers were used (4 cm, 5 cm, and 6 cm); for each length we varied the percentage by volume (0.3%, 0.5%, 0.8%, 1%, and 1.2%). To make a comparative study, a second series of tests was carried out with control concrete (BT) without any fibers of the same composition. The results of the tests carried out indicate that the resistance and stiffness are significantly improved and that the fibers imparted significant ductility to the material compared to the control concrete. It was also observed that the percentage of fibers significantly influences the appearance of cracks. Moreover, with an increase in the percentage of fibers from 0.3% to 0.8%, the resistance increases, but beyond a content of 0.8%, the mechanical characteristics decrease.
The Angle of the Shear Resistance of Danube Gravel Derived from the Dynamic Penetration Test
Authors: Kučová Eliška , Frankovská Jana
Published: December 2023

The dynamic penetration test was used to investigate the angle of shear strength of Danube gravel. The correlations for its determination are available in the standards and literature. From the data set of the results, the characteristic value of the angles of shear strength was derived according to the recommended statistical procedure from the second generation of Eurocode 7. The characteristic values of the angle of shear strength were evaluated for well-graded gravels, poor-graded gravels, and gravels with fine-grained particles of the Danube. The correlations, which were based on field tests and considered the genesis of the soil, were compared with laboratory test results and a table of the values from the Slovak standard, which are used for purposes of comparison.

In our research we compared the characteristic field values of the angle of shear strength obtained in the laboratory to observe the effect of their genesis on the appropriateness of the correlations used. We conclude with recommendations for the regional correlations and suggest values of the angle of shear strength for the classes of gravels tested.
The Metamorphosis of Marketing Communication Media: Strategy to Cultivate Digital Marketing Enthusiasm and Creativity in Batik Business Products
Authors: Suharyanto Deny Feri , Rasaili Wilda , Andiriyanto Ach. , , Zarnuji , Arista Rahman Sindi
Published: December 2023

The determination of technology has changed the face of the world in all sectors, the industrial and trade sectors in particular have a very significant impact. The technological revolution that touches all joints really forces all business people, in this case entrepreneurs, to move from conventional manual to digital. Even so, this internet technology revolution cannot suddenly get out of hand. It is necessary to test the readiness of the community to implement this digital technology. The research method uses descriptive qualitative through data collection by means of interviews, observation and FGD. The findings obtained are that the application of digital marketing is not optimal because it only relies on social networks. The implementation of digital marketing is carried out through stages, training and development followed by the implementation of programs that are adapted to the conditions at the Fahira Batik Collection. The results of the research are in the form of web-based e-commerce information technology development training and optimization of social media for craftsmen, training to improve the brand of Fahira Batik Collection products. So with this it is hoped that the craftsmen can expand their marketing reach by promoting their products through various social media so that it is expected to increase the income and welfare of the craftsmen.

Keywords: Digital Marketing, Commercialization, Technology, Communication
Crystal Palm Sugar Yield Optimization and Its Chemical and Sensory Characteristics
Authors: Marwati  , Febriandini Irma , Candra Krishna Purnawan , Yuliani  , Nurkaya Hamka
Published: December 2023

Crystal palm sugar (CPS) is crystal brown sugar produced from palm tree sap. The quality of CPS depends on the quality/freshness of the sap, while the sap quality depends on the tapping method and its freshness. This study aimed to determine the appropriate sap tapping time of Arenga palm (Arenga pinnata) for raw material in CPS processing. The sap tapping time was carried out at 3, 6, 9, and 12 hours, each with four replications. Sap tapping was done by adding five grams of sodium bicarbonate into the sap container. Parameters observed were sap pH, yield, chemical properties (moisture content, ash content, and reducing sugar content), and sensory properties (color, aroma, texture, and taste) of the CPS. The data were processed by ANOVA and polynomial regression. The results showed that sap tapping time significantly affected (p<0.05) the sap pH and reducing-sugar content of CPS, but not (p>0.05) the yield, water content, ash content, and sensory response of CPS. However, using quadratic polynomial regression shows that CPS yield is optimum by processing the palm sap at about seven hours of tapping time, i.e., 14.56% and 12.47% for coarse and fine CPS, respectively. The sap pH obtained from the seven hours of tapping time was 6.8-7.1. The CPS has a reducing sugar content of 26.94% and overall hedonic characteristics of moderately like. The hedonic quality characteristics of the CPS are typical palm sugar for aroma, slightly brown for color, slightly hard for texture, and sweet for taste.
MOLECULAR METHODS FOR IDENTIFYING SPECIES OF THE GENUS TUBENOSE GOBIES: CREATION AND APPLICATION OF PCR-PDF KEYS
Authors:   Scientific and Practical Center of the NAS of Belarus for Bioresources, Minsk, Golovenchik V.I. , Gajduchenko E.S. , Lipinskaya T.P.
Published: December 2023

The artical presents data about construction of PCR-PDF keys based on cytb gene to identify species of the genus Tubenose gobies. As a result of the gene analysis, 7 suitable restrictases out of 232 were selected. It is make it possible to identify each species of the genus Tubenose gobies during the restriction analysis. For each restrictase, PCR-RFLP tables and restriction maps were constructed.
EFFECT OF SODIUM DEOXYCHOLATE AND TRITON X-100 ON THE ACTIVITY OF NA,K-ATPASE OF THE MIXED MEMBRANE FRACTION OF THE CEREBRAL CORTEX OF RATS
Authors:   Institute of Biology, Tyumen, Deryagina E.V. , Makarova K.A.
Published: December 2023

Na+/K+-ATPase is an asymmetrically organized protein that maintains membrane potential. The active centers are intracellular; detection of activity without an increase in membrane permeability is not possible. The aim of the study was to study the activity of ouabain-sensitive and ouabain-resistant isoforms in the mixed membrane fraction of the cerebral cortex of rats exposed to the detergents Triton X-100 and Dox-Na.
DEVELOPMENT OF CRISPR-CAS12A BASED DIAGNOSTIC SYSTEM FOR GUANARITO VIRUS DETECTION COMBINED WITH ISOTHERMAL AMPLIFICATION IN ONE POT
Authors:   Pasteur Institute of Epidemiology and Microbiology, Saint Petersburg, Kapitonova M.A. , Shabalina A.V. , Dedkov V.G. , Dolgova A.S. ,   Saint Petersburg State University
Published: December 2023

CRISPR-Cas based diagnostic systems become more popular due to adaptability, simplicity and rapidness of analyses. In particular, the prospect of combining it with an isothermal amplification opens up new possibilities. In this work, we develop a detection system of the Guanarito virus based on Cas12a with RPA amplification in one pot. After several parameters optimization the analytical sensitivity is evaluated as 102 copies/µl.
NEW SEMISYNTHETIC SESQUITERPENE LACTONES AS INDUCERS OF OXIDATIVE STRESS IN TUMOR CELLS AND BLOCKERS OF THE AGGRESSIVE PHENOTYPE OF GLIOBLASTOMA MULTIFORME CELLS
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Kichkina D.O. , Patrushev S.S. , Moralev A.D. , Shults E.E. , Zenkova M.A. , Markov A.V. ,   Novosibirsk State University,   N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS
Published: December 2023

Screening for the antitumor potency of novel derivatives of isoalanotlactone revealed: (1) compound pat_651p, demonstrating high selectivity of action in respect to tumor cells and triggering oxidative stress and mitochondrial-dependent apoptosis in them; (2) compound pat_651_6p capable of passing through the blood-brain barrier and effectively inhibiting the motility, invasion, and adhesive traits of glioblastoma multiforme cells.
C1473G POLYMORPHISM IN THE MURINE TPH2 GENE REDUCES THE STABILITY OF TRYPTOPHAN HYDROXYLASE 2 ENZYME
Authors:   Institute of Cytology and Genetics SB RAS, Novosibirsk, Komleva P.D. , Arefieva A.B. , Sinyakova N.A. , Kulikov A.V.
Published: December 2023

C1473G polymorphism reduces the activity of tryptophan hydroxylase 2, a key enzyme of serotonin synthesis in the brain. Here we have showed that the C1473G polymorphism decreases temperature stability and free energy of the enzyme resulting in decreasing its stability, the number of active molecule and activity of the mutant TPH2.
CHANGES IN THE LANDSCAPE OF BREAST TUMOR UNDER THE ACTION OF ANTHRACYCLINE-CONTAINING NEOADJUVANT CHEMOTHERAPY REGIMENS
Authors:   Cancer Research Institute, Tomsk National Research Medical Center RAS, Kravtsova E.A. , Tsydenova I.A. , Dolgasheva D.S. , Gaptulbarova K.A. , Litviakov N.V. , Ibragimova M.K.
Published: December 2023

Breast cancer (BC) is the leading cause of cancer morbidity. A comprehensive approach, including the administration of neoadjuvant chemotherapy (NAC), is chosen when constructing a treatment regimen for breast cancer. In this regard, it is important to study the effects of different regimens of standard preoperative chemotherapy on the genetic landscape of the tumour, which determines both the favourable outcome and the stimulation of metastasis.
MODIFICATION OF AN ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS USING DIRECTED PROTEIN EVOLUTION
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Mikhaylova E.E. , Baykov I.K. , Tikunova N.V. ,   Novosibirsk State University
Published: December 2023

It is possible to obtain modified protein molecules with the properties necessary for the researcher by various methods. One of them is the directed evolution of proteins, which mimics the process of natural selection. The use of this method allowed to increase the affinity of the single-stranded antibody sc14D5 to the recombinant domain III of glycoprotein E of the European and Siberian subtypes of tick-borne encephalitis virus.
STUDY OF THE AUTONOMOUS STABILITY OF THE THREE-HELIX BUNDLE STRUCTURAL MOTIF
Authors:   Institute of Biomedical Chemistry, Moscow, Nikolsky K.S. , Kulikova L.E. , Petrovskiy D.V. , Rudnev V.R. , Kaysheva A.L.
Published: December 2023

This work is devoted to the study of the autonomous stability of the three-helix bundle structural motif, which is often found in functionally diverse protein classes. The study was carried out on a large set of protein structures selected from the bank (PDB) with a spatial packing of the three-helix bundle. With the help of a molecular dynamics experiment, the autonomous stability of the studied folds outside the protein in the aquatic environment was shown.
CELL RECEPTORS AND SIGNALING PATHWAYS INVOLVED IN THE REGENERATION OF INJURED PERIPHERAL NERVES
Authors:   Ogarev Mordovia State University, Saransk, Otryaskin Ya.S. , Yurtova A.V. , Pinyaev S.I.
Published: December 2023

The paper considers the process of injury to peripheral nerve fibers and the possibility of influencing their regeneration through reactions mediated by interaction with receptors that activate signaling pathways. Pathways such as RAS/ERK, PI3K, PLC-γ, JAK-STAT, MAPK/ERK, JNK and p38MAPK are key in signal transduction through biochemical cascade reactions, and modulating them can speed up the process of repair of damaged nerve fibers.
COMPARISON OF COLLATERAL ACTIVITY OF CRISPR CS12A ORTHOLOGS FOR THE DEVELOPMENT OF NOVEL DIAGNOSTIC SYSTEMS
Authors:   Peter the Great Saint Petersburg Polytechnic University, Potyseva A.S. , Arseniev A.N. , Selkova P.A. , Vasilieva A.A. , Melnikov A.S. , Serdobintsev P.Yu. , Khodorkovskii M.A.
Published: December 2023

CRISPR/Cas systems are defense systems for bacteria and archaea. Several classes of these systems have now been characterized. In addition to the main directed nuclease activity, type II class V systems possess specific collateral activity, which formed the basis for a new type of diagnostic systems. In the result of this work we obtained data characterizing the collateral activity of two orthologues of this class and demonstrated that the CRISPR system ScCas12a has greater specificity compared to AsCas12a.
EFFECT OF ACTIVATION OF MYC GENE EXPRESSION ON A METASTASIS POTENTIAL OF A BREAST CANCER CELL LINE
Authors:   Siberian State Medical University, Tomsk, Sukhinina E.V. , Kozlova P.K. , Nevskaya K.V. , Litvyakov N.V. , Pershina A.G. ,   Tomsk Polytechnic University,   Tomsk State University,   Cancer Research Institute, Tomsk NRMC
Published: December 2023

The overexpression of the MYC gene plays a crucial role in the advancement of tumors and the plasticity of malignant cells. This study aimed to create breast cancer cell line BT-549 with activated MYC gene, using the CRISPR/Cas9-based SAM activation technique. The modified cell line demonstrates an increase in the fraction of CD44+ CD24– cells, as well as increase in the number and diameter of mammospheres formed. These studies open new opportunities for investigation of the mechanisms related to metastasis.
HIGH-YIELD PRODUCTION OF “DIFFICULT-TO-EXPRESS” PROTEINS ARDA AND ARDB WITH THE THERMO-INDUCIBLE VECTOR PIR-DPAL
Authors:   Moscow Institute of Physics and Technology, Dolgoprudny, Utkina A.A. , Kudryavtseva A.A. , Manuhkov I.V.
Published: December 2023

The antirestriction proteins ArdA and ArdB inhibit type I restriction-modification (RM) systems. Such proteins are usually difficult to obtain in Escherichia coli cells in large quantities since they are toxic to the host cell. This work is devoted to the cloning of the ardA and ardB antirestrictase genes into the thermo-inducible vector pIR-DPAl vector, which made it possible to increase the efficiency of its expression and purification.
Comparação entre duas técnicas para impactação de marcapasso provisório na sala de urgência
Authors: Holanda Vitor Bruno Texeira de , Holanda Lucianna Sefarty de , Vieira Junior Feliciano Mendes , Freitas Derlon Silva de , Coutinho Rafael Maia , Coutinho Fernando Maia , Rodrigues Emily Saboia Moura , Bezerra Mayra Ferreira , Marvão Márcio César Ribeiro
Published: December 2023

Objetivo: Avaliar o uso do USG à beira do leito, como guia para o posicionamento rápido e correto do eletrodo do marcapasso, em comparação ao método às cegas. Métodos: Estudo observacional retrospectivo realizado em um único centro, com dois grupos, o primeiro guiado por ultrassonografia e o outro cego. A escolha da técnica utilizada ficou a critério do profissional executor. A amostra foi composta por 60 pacientes internados no período do agosto a outubro de 2020, sendo 28 guiados por ultrassonografia e 32 cegos. Resultados: No grupo cego, a taquicardia ventricular foi a mais frequente (6,3%), em um grupo houve 1 caso de perfuração cardíaca e óbito. No tempo para impactação observou-se diferença estatisticamente significante (*p = 0,0044) entre os grupos avaliados. No grupo com ultrassonografia, a maior proporção de pacientes obteve tempo de impactação inferior a 1 minuto (35,7%). Conclusão: A estratégia do uso da ultrassonografia diminui a taxa de taquicardia ventricular, além de reduzir substancialmente o tempo de impactação para menos de 1 minuto e zerar a necessidade de transferência para hemodinâmica.