bluuebunny/crossref_metadata_2025 huggingface dataset:
A new immunodeficient Duchenne muscular dystrophy rat model to evaluate engraftment after human cell transplantation
Authors: Sato Masae , Goto Megumi , Yamanouchi Keitaro , Sakurai Hidetoshi
Published: April 2023

Duchenne muscular dystrophy (DMD) is an X-linked fatal muscular disease, affecting one in 3,500 live male births worldwide. Currently, there is no cure for this disease, except for steroid-based treatment to attenuate disease progression. Cell transplantation therapy is a promising therapeutic approach, however, there is a lack of appropriate animal models to conduct large-scale preclinical studies using human cells, including biochemical and functional tests. Here, we established an immunodeficient DMD rat model and performed exhaustive pathological analysis and transplantation efficiency evaluation to assess its suitability to study DMD. Our DMD rat model exhibited histopathological characteristics similar to those observed in human patients with DMD. Human myoblasts demonstrated successful engraftment following transplantation into these rats. Therefore, this immunodeficient DMD rat model would be useful in preclinical studies to develop cellular transplantation therapies for DMD.
Overview of programmed electrical stimulation to assess atrial fibrillation susceptibility in mice
Authors: Murphy Matthew B. , Kannankeril Prince J. , Murray Katherine T.
Published: April 2023

Atrial fibrillation (AF) is the most common human arrhythmia and is associated with increased risk of stroke, dementia, heart failure, and death. Among several animal models that have been used to investigate the molecular determinants of AF, mouse models have become the most prevalent due to low cost, ease of genetic manipulation, and similarity to human disease. Programmed electrical stimulation (PES) using intracardiac or transesophageal atrial pacing is used to induce AF as most mouse models do not develop spontaneous AF. However, there is a lack of standardized methodology resulting in numerous PES protocols in the literature that differ with respect to multiple parameters, including pacing protocol and duration, stimulus amplitude, pulse width, and even the definition of AF. Given this complexity, the selection of the appropriate atrial pacing protocol for a specific model has been arbitrary. Herein we review the development of intracardiac and transesophageal PES, including commonly used protocols, selected experimental models, and advantages and disadvantages of both techniques. We also emphasize detection of artifactual AF induction due to unintended parasympathetic stimulation, which should be excluded from results. We recommend that the optimal pacing protocol to elicit an AF phenotype should be individualized to the specific model of genetic or acquired risk factors, with an analysis using several definitions of AF as an endpoint.
Preoperative state anxiety predicts postoperative health-related quality of life: A prospective observational study on patients undergoing lung cancer surgery
Authors: Takamiya Shinnosuke , Honma Motoyasu , Masaoka Yuri , Okada Momoka , Ohashi Shinichi , Tanaka Yoko , Suzuki Kosuke , Uematsu Shugo , Kitami Akihiko , Izumizaki Masahiko
Published: April 2023

Objective

Improving quality of life (QOL) after surgery is very important. Recently, preoperative anxiety has been suggested to predict postoperative health-related (HR) QOL, however the accuracy of anxiety measurement remains problematic. We examined the relationship between preoperative anxiety level and postoperative HRQOL using qualitative and quantitative assessment of anxiety.

Method

We used a detailed anxiety assessment to quantitatively investigate preoperative anxiety as a predictor of postoperative HRQOL in lung cancer patients. Fifty one patients who underwent surgery for lung cancer were included. They were assessed four times: on admission, on discharge, 1 month after surgery, and 3 months after surgery. Anxiety was measured separately as “state anxiety” and “trait anxiety” using the State–Trait Anxiety Inventory, and HRQOL was measured using the EuroQol 5 dimension 5-level.

Results

The HRQOL decreased at discharge and gradually recovered over time, reaching the same level at 3 months after surgery as at admission. HRQOL score was lower at discharge than at pre-surgery and 3 months after the surgery (p < 0.0001 each), and the score at 1 month after the surgery was lower than at pre-surgery (p = 0.007). In addition, multiple regression analysis showed that HRQOL at discharge was associated with “state anxiety” rather than “trait anxiety” at admission (p = 0.004).

Conclusion

This study identifies the types of anxiety that affect postoperative HRQOL. We suggest that postoperative HRQOL on discharge may be improved by interventions such as psychological or medication treatment for preoperative state anxiety if identified preoperative state anxiety can be managed appropriately.
Development of the sickle Pan-African research consortium registry in Tanzania: opportunity to harness data science for sickle cell disease
Authors: Kandonga Daniel , Sangeda Raphael Zozimus , Masamu Upendo , Kazumali Eliah , Jonathan Agnes , Msangawale Michael , Kaihula Winfrida , Rwegalulila Julieth , Ondego Jesca , Tutuba Hilda J. , Ndunguru Joyce , Ambrose Emmanuela E. , Kidenya Benson R. , Yonazi Mbonea , Kyomugisha Irene , Mupfururirwa Wilson , Jonas Mario , Nembaware Victoria , Mazandu Gaston Kuzamunu , Kengne Andre Pascal , Wonkam Ambroise , Makani Julie , Balandya Emmanuel
Published: April 2023

Background

Sickle cell disease (SCD) is a severe hereditary form of anemia that contributes between 50% and 80% of under-five mortality in Africa. Eleven thousand babies are born with SCD annually in Tanzania, ranking 4th after Nigeria, the Democratic Republic of Congo and India. The absence of well-described SCD cohorts is a major barrier to health research in SCD in Africa.

Objective

This paper describes the Sickle Pan African Consortium (SPARCO) database in Tanzania, from the development, design of the study instruments, data collection, analysis of data and management of data quality issues.

Methods

The SPARCO registry used existing Muhimbili Sickle Cell Cohort (MSC) study case report forms (CRF) and later harmonized data elements from the SickleInAfrica consortium to develop Research Electronic Data Capture (REDCap) instruments. Patients were enrolled through various strategies, including mass screening following media sensitization and health education events during World Sickle Cell Day each June and the SCD awareness month in September. Additional patients were identified through active surveillance of previously participating patients in the MSC.

Results

Three thousand eight hundred patients were enrolled between October 2017 and May 2021. Of these, 1,946 (51.21%) were males and 1,864 (48.79%) were females. The hemoglobin phenotype distribution was 3,762 (99%) HbSS, 3 (0.08%) HbSC and 35 (0.92%) HbSβ +thalassemia. Hemoglobin levels, admission history, blood transfusion and painful events were recorded from December 2017 to May 2021.

Conclusion

The Tanzania SPARCO registry will improve healthcare for SCD in Africa through the facilitation of collaborative data-driven research for SCD.
Learning hybrid locomotion skills—Learn to exploit residual actions and modulate model-based gait control
Authors: Kasaei Mohammadreza , Abreu Miguel , Lau Nuno , Pereira Artur , Reis Luis Paulo , Li Zhibin
Published: April 2023

This work has developed a hybrid framework that combines machine learning and control approaches for legged robots to achieve new capabilities of balancing against external perturbations. The framework embeds a kernel which is a model-based, full parametric closed-loop and analytical controller as the gait pattern generator. On top of that, a neural network with symmetric partial data augmentation learns to automatically adjust the parameters for the gait kernel, and also generate compensatory actions for all joints, thus significantly augmenting the stability under unexpected perturbations. Seven Neural Network policies with different configurations were optimized to validate the effectiveness and the combined use of the modulation of the kernel parameters and the compensation for the arms and legs using residual actions. The results validated that modulating kernel parameters alongside the residual actions have improved the stability significantly. Furthermore, The performance of the proposed framework was evaluated across a set of challenging simulated scenarios, and demonstrated considerable improvements compared to the baseline in recovering from large external forces (up to 118%). Besides, regarding measurement noise and model inaccuracies, the robustness of the proposed framework has been assessed through simulations, which demonstrated the robustness in the presence of these uncertainties. Furthermore, the trained policies were validated across a set of unseen scenarios and showed the generalization to dynamic walking.
Robotic gaze and human views: A systematic exploration of robotic gaze aversion and its effects on human behaviors and attitudes
Authors: Koller Michael , Weiss Astrid , Hirschmanner Matthias , Vincze Markus
Published: April 2023

Similar to human–human interaction (HHI), gaze is an important modality in conversational human–robot interaction (HRI) settings. Previously, human-inspired gaze parameters have been used to implement gaze behavior for humanoid robots in conversational settings and improve user experience (UX). Other robotic gaze implementations disregard social aspects of gaze behavior and pursue a technical goal (e.g., face tracking). However, it is unclear how deviating from human-inspired gaze parameters affects the UX. In this study, we use eye-tracking, interaction duration, and self-reported attitudinal measures to study the impact of non-human inspired gaze timings on the UX of the participants in a conversational setting. We show the results for systematically varying the gaze aversion ratio (GAR) of a humanoid robot over a broad parameter range from almost always gazing at the human conversation partner to almost always averting the gaze. The main results reveal that on a behavioral level, a low GAR leads to shorter interaction durations and that human participants change their GAR to mimic the robot. However, they do not copy the robotic gaze behavior strictly. Additionally, in the lowest gaze aversion setting, participants do not gaze back as much as expected, which indicates a user aversion to the robot gaze behavior. However, participants do not report different attitudes toward the robot for different GARs during the interaction. In summary, the urge of humans in conversational settings with a humanoid robot to adapt to the perceived GAR is stronger than the urge of intimacy regulation through gaze aversion, and a high mutual gaze is not always a sign of high comfort, as suggested earlier. This result can be used as a justification to deviate from human-inspired gaze parameters when necessary for specific robot behavior implementations.
Stress quantification in a composite matrix via mechanophores
Authors: Gohl Jared A. , Wiley Tristan J. , Chang Hao-Chun , Chang Chia-Chih , Davis Chelsea S.
Published: April 2023

Stress concentrations in polymer matrix composites occur due to non-uniform loadings which develop near the interface between the matrix and reinforcement in a stressed composite. Methods to better understand the evolution of this stress concentration are required for the development of advanced composites. Mechanophores, which are stress responsive molecules, can be embedded into the polymer matrix and used to quantify the local stresses in a loaded composite. In this work, single particle model composites were fabricated by combining functionalized glass particles embedded into a silicone/mechanophore matrix. Confocal microscopy was then used to measure the mechanophore activation in situ during mechanical loading. The fluorescence intensity was correlated to maximum principal stress values obtained from a finite element analysis (FEA) model of the system utilizing an Ogden hyperelastic model to represent the elastomer. By calibrating stress to fluorescence intensity spatially, quantitative stress measurements can be obtained directly from fluorescent images. To validate this technique, calibrated stress values for a two-particle composite system were compared to a FEA model and good agreement was found. Further experiments were performed on silicone matrix composites containing short cylindrical particles oriented with their major axis parallel or perpendicular to the stretching direction. To demonstrate the versatility of the single particle intensity/stress calibration approach, maximum principal stress values were mapped on the fluorescence images of the cylindrical experiments. This technique has potential to quantify stress concentrations quickly and accurately in new composite designs without the use of FEA models or differential image correlation.
A Numerical Study on the Flap Side-Edge Noise Reduction Using Passive Blowing Air Concept
Authors: Zhang Yingzhe , Bai Baohong , Lin Dakai , Liu Peiqing
Published: April 2023

The flap side-edge is a vital contributor to airframe noise. In this study, we propose a novel flap side-edge noise reduction method based on the concept of active blowing air. A long slot is opened from the flap’s lower surface to the tip surface to induce a secondary jet flow, which is driven by the local pressure difference between the flap’s lower surface and the tip surface. The unsteady flow field around the flap side-edge was computed by the lattice Boltzmann solver PowerFLOW, and the far-field noise was predicted by the FW-H equation. It is demonstrated that the dominant features of the flap side-edge flow are the double vortex structures, and the new passive blowing air reduction method can achieve about 3.3 dB noise reduction. Moreover, the underlying noise reduction mechanism has been analyzed and revealed. It is shown that the secondary jet flow from the long slot on the flap side-edge would dissipate the flap side-edge vortex and displace the flap side-edge vortical structure away from the flap surface, thus resulting in a decrease in the pressure fluctuations on the flap side-edge surface. As a result, the flap side-edge noise was reduced. In contrast to the current active air blowing technique, the newly proposed blowing air technique is passive and quite simple and does not require an extra air source or control system. This novel flap side-edge noise reduction technology provides a new flow control strategy and noise reduction methodology and can be further optimized.
Study of Plasma-Based Vortex Generator in Supersonic Turbulent Boundary Layer
Authors: Polivanov Pavel , Vishnyakov Oleg , Sidorenko Andrey
Published: April 2023

The problem of flow control under conditions of a turbulent boundary layer at transonic and supersonic free-stream velocities is considered. Such flows are integral components of the flight process and exert significant effects on the flow around both the aerodynamic object as a whole and its individual elements. The present paper describes investigations of a combined control device (“plasma wedge”), which is a wedge mounted along the flow with the energy supply at one side of the wedge owing to a spark discharge. The strategy of flow control by this device is based on increasing the momentum in the boundary layer, which enhances its resistance to the adverse pressure gradient and, as a consequence, its resistance to flow separation further downstream. The study includes experimental and computational aspects. The examined flow evolves on a rectangular flat plate with a sharp leading edge at the free-stream Mach number M = 1.45 and unit Reynolds numbers Re1 = 11.5·106 1/m. The experiments are performed to study the velocity fields and the pressure distribution in the wake behind the actuator. The results show that a streamwise vortex is formed in the wake behind the actuator when the discharge is initiated. Reasonable agreement of the experimental data with numerical simulations allows one to conclude that the Reynolds-averaged Navier–Stokes equations are suitable tools for solving the problem considered.
Development of a Low-Power Automatic Monitoring System for Spodoptera frugiperda (J. E. Smith)
Authors: Chen Meixiang , Chen Liping , Yi Tongchuan , Zhang Ruirui , Xia Lang , Qu Cheng , Xu Gang , Wang Weijia , Ding Chenchen , Tang Qing , Wu Mingqi
Published: April 2023

Traditional traps for Spodoptera frugiperda (J. E. Smith) monitoring require manual counting, which is time-consuming and laborious. Automatic monitoring devices based on machine vision for pests captured by sex pheromone lures have the problems of large size, high power consumption, and high cost. In this study, we developed a micro- and low-power pest monitoring device based on machine vision, in which the pest image was acquired timely and processed using the MATLAB algorithm. The minimum and maximum power consumption of an image was 6.68 mWh and 78.93 mWh, respectively. The minimum and maximum days of monitoring device captured image at different resolutions were 7 and 1486, respectively. The optimal image resolutions and capture periods could be determined according to field application requirements, and a micro-solar panel for battery charging was added to further extend the field life of the device. The results of the automatic counting showed that the counting accuracy of S. frugiperda was 94.10%. The automatic monitoring device had the advantages of low-power consumption and high recognition accuracy, and real-time information on S. frugiperda could be obtained. It is suitable for large-scale and long-term pest monitoring and provides an important reference for pest control.
Toward Sustainable Farming: Implementing Artificial Intelligence to Predict Optimum Water and Energy Requirements for Sensor-Based Micro Irrigation Systems Powered by Solar PV
Authors: Mohammed Maged , Hamdoun Hala , Sagheer Alaa
Published: April 2023

Future trends in climate change, water scarcity, and energy costs will motivate agriculturists to develop innovative agricultural systems. In order to achieve sustainable farming in arid regions, there is an urgent need to use artificial intelligence (AI) to predict and estimate the optimum water and energy requirements for the irrigation of date palms. Therefore, this study aimed to predict the optimum water and energy requirements for date palm irrigation depending on the optimum water use efficiency (WUE) and yield in arid conditions. To achieve this aim, four solar-powered micro irrigation systems were developed and evaluated under six irrigation levels for date palm irrigation. Soil moisture sensor-based controllers were used to automate irrigation scheduling for the micro irrigation systems. The water pumping in these systems was powered using a solar photovoltaic (PV) system. In addition, four machine-learning (ML) algorithms, including linear regression (LR), support vector regression (SVR), long short-term memory (LSTM) neural network, and extreme gradient boosting (XGBoost), were developed and validated for prediction purposes. These models were developed in Python programing language using the Keras library. The results indicated that the optimum WUS was achieved when the maximum setpoints of irrigation control were adjusted at the field capacity and by adjusting the minimum setpoints at 40, 50, 70, and 80% of the available water (AW). The optimum yield was achieved by adjusting the minimum setpoints at 60, 70, 80, and 90% of AW for subsurface irrigation, subsurface drip irrigation, drip irrigation, and bubbler irrigation, respectively. Therefore, the dataset was prepared at these levels for four years to train and test the models, and a fifth year was used to validate the performance of the best model. The evaluation of the models showed that the LSTM followed by XGBoost models were more accurate than the SVR and LR models for predicting the optimum irrigation water and energy requirements. The validation result showed that the LSTM was able to predict the water and energy requirements for all irrigation systems with R2 ranging from 0.90 to 0.92 based on limited meteorological variables and date palm age. The findings of the current study demonstrated that the developed LSTM model can be a powerful tool in irrigation water and energy management as a fast and easy-to-use approach.
Effect of Drip Irrigation, N, K, and Zn Coupling on Pn of Densely Cultivated Apple on Dwarf Rootstock in Xinjiang, China
Authors: Mu Weiyi , Duan Xiaoxian , Wang Quanjiu , Wei Xuesong , Wei Kai , Li Yuanxin , Guo Xin
Published: April 2023

This study aimed to determine the effect of irrigation amount (W), nitrogen (N), potassium (K), and zinc (Zn) on the net photosynthetic rate (Pn) of closely planted apple trees on dwarf rootstocks in arid areas of Xinjiang. Taking the “Royal Gala” apple as the experimental material, a mathematical model for Pn was established using the principle of four-factor five-level quadratic regression with a general rotation combination design. The results show that: (1) The regression equations reached significant levels (F = 37.06 > F0.01(11.11) = 4.54). (2) The effect of W, N, K, Zn on Pn is significant with relative importance W > N > Zn > K. (3) The results of single factor analysis showed that with an increase in W, N, K, and Zn, Pn exhibits an n-shaped parabolic response. (4) The positive coupling between W and N is significant, and the positive coupling between W and Zn is also significant. (5) Analysis of the interaction between sets of three factors revealed that W, N, and Zn could be combined to best effect, with the maximum value reaching 12.77 μmol·m−2·s−1. Compared with W×K×Zn and W×N×K, the combination of W×N×Zn reduces W by 9.2% and 6.3%, respectively, which indicates its suitability for use in the dry and water deficient planting environment in Xinjiang. (6) Within the 95% confidence level, when W is 258–294.75 mm, N is 33.44–39.51 kg/hm2, K is 53.82–69.39 kg/hm2, and Zn is 6.46–7.84 kg/hm2, the net photosynthetic rate reaches 11 μmol·m−2·s−1.
Quantitative Analysis of Source-Sink Relationships in Two Potato Varieties under Different Nitrogen Application Rates
Authors: Liu Kunyu , Meng Meilian , Zhang Tingting , Chen Youjun , Yuan Haotian , Su Taimin
Published: April 2023

Nitrogen is an essential nutrient for plant growth. However, the excessive use of nitrogen fertilizers not only increases production cost, but also has negative a impact on the environment. The purpose of this study was to quantify the source-sink characteristics and length of each growth stage in two potato varieties under different nitrogen application rates. This clarifies the source-sink coordination characteristics of the nitrogen-efficient variety and the source-sink coordination mechanisms of high nitrogen use efficiency (NUE). Field experiments were conducted in 2019, 2020, and 2021 using a split-plot design, with a nitrogen application rate of (0; 150 kg·ha−1; 300 kg·ha−1) as the main plot and variety (J, nitrogen-efficient variety Jizhang 12; Y, nitrogen-inefficient variety Youjia 70) as the subplot. The results showed that the yield and NUE of Jizhang 12 at 300 kg·ha−1 were, on average, 90.73% and 75.15% higher than those of Youjia 70, respectively. The NUE and nitrogen utilization efficiency of Jizhang 12 increased on average, with decreasing N application at 68.66% and 24.53%, which were higher than those of Youjia 70 at 62.89% and 10.86%. Quantitative analysis of the source and sink showed that the Jizhang 12 had a higher source and sink capacity of 23.45 g and 51.85 g, respectively, and the maximum source and sink activity was on average 0.28 g·plant−1·d−1 and 1.47 g·plant−1·d−1 higher, and the growth period of the source and sink was on average 24 days and 7 days longer, respectively. On the basis of these results, the nitrogen-efficient varieties had a higher yield base and a smaller reduction in NUE with reduced N application. In terms of source-sink growth, N-efficient varieties lasted longer at the seedling and tuber initiation stages, when potatoes grew above ground and source organs grew for longer periods, providing a solid foundation for later sink growth, as evidenced by their higher source-sink activity, capacity, and growth time than N-inefficient varieties.
Metric Ensembles Aid in Explainability: A Case Study with Wikipedia Data
Authors: Forbes Grant , Crouser R. Jordan
Published: April 2023

In recent years, as machine learning models have become larger and more complex, it has become both more difficult and more important to be able to explain and interpret the results of those models, both to prevent model errors and to inspire confidence for end users of the model. As such, there has been a significant and growing interest in explainability in recent years as a highly desirable trait for a model to have. Similarly, there has been much recent attention on ensemble methods, which aim to aggregate results from multiple (often simple) models or metrics in order to outperform models that optimize for only a single metric. We argue that this latter issue can actually assist with the former: a model that optimizes for several metrics has some base level of explainability baked into the model, and this explainability can be leveraged not only for user confidence but to fine-tune the weights between the metrics themselves in an intuitive way. We demonstrate a case study of such a benefit, in which we obtain clear, explainable results based on an aggregate of five simple metrics of relevance, using Wikipedia data as a proxy for some large text-based recommendation problem. We demonstrate that not only can these metrics’ simplicity and multiplicity be leveraged for explainability, but in fact, that very explainability can lead to an intuitive fine-tuning process that improves the model itself.
Duplicated Inferior Vena Cava in a 69-Year-Old White Female Donor
Authors: Klansek Joanna , Meshida Keiko , Maynes Elizabeth , Leighton Maria Ximena , Wind Gary , Granite Guinevere
Published: April 2023

While relatively uncommon, a duplication of the inferior vena cava is moderately well-discussed in the literature. This anatomical variation was noted in a 69-year-old white female donor. This variation is typically asymptomatic; however, it can be associated with complications, such as confusion with a mediastinal mass, increased risk for thromboembolism, and hemorrhage during surgery. It is also associated with a handful of comorbidities, including, but not limited to, congenital renal anomalies such as horseshoe kidney or fused crossed kidney. Research supports that the variation of a duplicated IVC (DIVC) can be due to a failure of the left supracardinal vein to regress during embryonic development.
Oral Palatability and Owners’ Perception of the Effect of Increasing Amounts of Spirulina (Arthrospira platensis) in the Diet of a Cohort of Healthy Dogs and Cats
Authors: Stefanutti Davide , Tonin Gloria , Morelli Giada , Zampieri Raffaella Margherita , La Rocca Nicoletta , Ricci Rebecca
Published: April 2023

The nutraceutical supplementation of Spirulina (Arthrospira platensis) in dogs and cats has not yet been investigated. The aim of this study was to evaluate if the dietary supplementation of increasing amounts of Spirulina for 6 weeks is palatable to pets and to assess the owner’s perception of such supplementation. The owners of the 60 dogs and 30 cats that participated in this study were instructed to daily provide Spirulina tablets starting with a daily amount of 0.4 g, 0.8 g, and 1.2 g for cats as well as small dogs, medium dogs, and large dogs, respectively, and allowing a dose escalation of 2× and 3× every 2 weeks. The daily amount (g/kg BW) of Spirulina ranged from 0.08 to 0.25 for cats, from 0.06 to 0.19 for small-sized dogs, from 0.05 to 0.15 for medium-sized dogs, and from 0.04 to 0.12 for large-sized dogs. Each owner completed a questionnaire at the time of recruitment and the end of each 2-week period. No significant effect on the fecal score, defecation frequency, vomiting, scratching, lacrimation, general health status, and behavioral attitudes was detected by the owners’ reported evaluations. Most animals accepted Spirulina tablets either administrated alone or mixed with food in the bowl. Daily supplementation of Spirulina for 6 weeks in the amounts provided in this study is therefore palatable and well tolerated by dogs and cats.
Effects of Pueraria Extract and Curcumin on Growth Performance, Antioxidant Status and Intestinal Integrity of Broiler Chickens
Authors: Guo Shuangshuang , Hu Jinchao , Ai Sihan , Li Lanlan , Ding Binying , Zhao Di , Wang Lei , Hou Yongqing
Published: April 2023

The current study was carried out to examine the effects of pueraria extract (PE) and curcumin (CUR) on growth performance, antioxidant capacity and intestinal integrity in broiler chickens. A complete randomized design with a 2 × 2 factorial arrangement of treatments was employed to assign 200 one-day-old Ross-308 broilers to four groups, each including five replicates of ten birds. Chickens in the control group (CON) were fed the basal diet, while the PE, CUR and PE+CUR groups were supplemented with 200 mg/kg PE or 200 mg/kg CUR or 200 mg/kg PE+ 200 mg/kg CUR. This trial lasted for 28 days. The PE supplementation decreased the average daily gain during the whole period (p < 0.05). The PE+CUR group had a higher feed conversion ratio than that of the PE and CUR groups during days 14–28 and 1–28 (p < 0.05). Dietary CUR supplementation increased duodenal T-SOD activity (p < 0.05). Compared with the CON group, the other three groups increased the duodenal GSH-Px activity, the PE+CUR group reduced the duodenal H2O2 level, and the CUR and PE groups elevated the ileal GSH-Px activity and the ratio of jejunal villus height to crypt depth, respectively (p < 0.05). The addition of PE decreased crypt depth and increased villus area and mucin-2 mRNA level in the jejunum (p < 0.05). Overall, dietary supplementation with PE, CUR, or a combination of these, enhanced the antioxidant status and intestinal integrity of broilers.
Review of the Effects of Enclosure Complexity and Design on the Behaviour and Physiology of Zoo Animals
Authors: de Azevedo Cristiano Schetini , Cipreste Cynthia Fernandes , Pizzutto Cristiane Schilbach , Young Robert John
Published: April 2023

The complexity of the habitat refers to its physical geometry, which includes abiotic and biotic elements. Habitat complexity is important because it allows more species to coexist and, consequently, more interactions to be established among them. The complexity of the habitat links the physical structure of the enclosure to the biological interactions, which occur within its limits. Enclosure complexity should vary temporally, to be able to influence the animals in different ways, depending on the period of the day and season and throughout the year. In the present paper, we discuss how habitat complexity is important, and how it can positively influence the physical and mental states of zoo animals. We show how habitat complexity can ultimately affect educational projects. Finally, we discuss how we can add complexity to enclosures and, thus, make the lives of animals more interesting and functional.
Fecal Metabolomics Reveals the Foraging Strategies of Giant Pandas for Different Parts of Bamboo
Authors: Yan Zheng , Xu Qin , Yao Ying , Ayala James , Hou Rong , Wang Hairui
Published: April 2023

Climate change-induced food shortages pose major threats to wildlife conservation, and the exclusive reliance of giant pandas on bamboo makes them particularly vulnerable. The aim of this study was to provide insight into the reasons for the foraging strategies of giant pandas to selectively forage for different bamboo parts (bamboo shoot, culm, and leaf) during different seasons. This study used a metabolomic approach to analyze the fecal metabolites of giant pandas and conducted a correlation analysis with their gut microbiota. The results indicate that the fecal metabolites of giant pandas differ significantly depending on the bamboo parts they forage on, with higher sugar content observed when they consume bamboo culm with high fiber content. By functional annotation, culm group metabolites were enriched in the galactose metabolic pathway, while shoot group metabolites were enriched in the phenylalanine, tyrosine and tryptophan biosynthesis pathways. Moreover, Streptococcus showed a significant positive correlation with glucose and acetic acid content. Therefore, the foraging strategy of giant pandas is based on the ability to utilize the nutrient content of different bamboo parts. Captive feeding and habitat construction should enrich bamboo species to allow them to express their natural foraging strategies and improve their welfare and reproductive status.
Where Are We Now? Feeds, Feeding Systems and Current Knowledge of UK Horse Owners When Feeding Haylage to Their Horses
Authors: Moore-Colyer Meriel , Westacott Amy , Rousson Lucile , Harris Patricia , Daniels Simon
Published: April 2023

Despite our improved understanding of equid digestive health and accurate rations formulations, obesity in the UK horse population remains high. Study aims: (1) to determine how owners are feeding their horses and what influences their choices, (2) to understand owners’ knowledge of haylage and (3) to identify key areas that require additional education. Data were collected in 2020 from 1338 UK horse owners via two online surveys. Survey 1 was on general feeding practices, and Survey 2 was specifically on the feeding of haylage. Data were processed using chi square analyses + Bonferroni tests, with a significance p < 0.05. Equal numbers of leisure and performance horse owners completed both surveys. For Survey 1, 67% fed hay as the only forage, 30% fed forage (hay/haylage) + balancer, 36% fed haylage and hay to manage energy intake, 84% added a cube or coarse mix, 88% did not do forage analyses, 74% did not see the need for it and 16% did not know analyses could be done. In Survey 2, those who were not feeding haylage, 66% were not sure how to feed it, 68% worried about aerobic spoilage and 79% said the bale size was unsuitable. Body weight measurements (Survey 1 and Survey 2) were rarely performed (11%). Aspects of ration formulations, the value of feed analyses and how to interchange hay and haylage require additional education to owners for improved ration compilation.
Size-Mediated Trophic Interactions in Two Syntopic Forest Salamanders
Authors: Costa Andrea , Rosa Giacomo , Salvidio Sebastiano
Published: April 2023

Exploitative competition and interference competition differ in the way they affect re-source availability for competitors: in the former, organisms reduce resource availability for the competitors; in the latter, one organism actively prevents the competitor from accessing resources, independently of their availability. Our aim is to test for the presence of foraging competition in two forest-dwelling salamanders in Italy: Speleomantes strinatii and Salamandrina perspicillata. We also aim at testing for size-mediated competition. We obtained stomach contents from 191 sampled individuals by means of stomach flushing at 8 sampling sites where both species occur. We focused our analysis on the core prey taxa shared by both species: Collembola and Acarina. We found that the foraging activity of S. perspicillata is positively affected by body size and negatively affected by potential competitor’s activity on the forest floor during the sampling, which also significantly weakened the positive relationship with body size. These results suggest the presence of an interference/interaction occurring between the two species and affecting the foraging activity of S. perspicillata. This competitive interaction is size mediated and configured as interference competition rather than exploitative competition.
Genetic Heterogeneity and Mutated PreS Analysis of Duck Hepatitis B Virus Recently Isolated from Ducks and Geese in China
Authors: Xu Shuqi , Mu Xinhao , Xu Xin , Bi Congying , Ji Jun , Kan Yunchao , Yao Lunguang , Bi Yingzuo , Xie Qingmei
Published: April 2023

In this study, we detected 12 duck and 11 goose flocks that were positive for duck hepatitis B virus (DHBV) using polymerase chain reaction and isolated 23 strains between 2020 and 2022 in China. The complete genomes of goose strains E200801 and E210501 shared the highest identity (99.9%), whereas those of strains Y220217 and E210526 shared the lowest identity (91.39%). The phylogenetic tree constructed based on the genome sequences of these strains and reference strains was classified into three major clusters: the Chinese branch DHBV-I, the Chinese branch DHBV-II, and the Western branch DHBV-III. Furthermore, the duck-origin strain Y200122 was clustered into a separate branch and was predicted to be a recombinant strain derived from DHBV-M32990 (belonging to the Chinese branch DHBV-I) and Y220201 (belonging to the Chinese branch DHBV-II). Additionally, preS protein analysis of the 23 DHBV strains revealed extensive mutation sites, almost half of which were of duck origin. All goose-origin DHBV contained the mutation site G133E, which is related to increased viral pathogenicity. These data are expected to promote further research on the epidemiology and evolution of DHBV. Continuing DHBV surveillance in poultry will enhance the understanding of the evolution of HBV.
In Vitro Characterization of Probiotic Potential of Limosilactobacillus fermentum against Salmonella Gallinarum Causing Fowl Typhoid
Authors: Mehmood Adnan , Nawaz Muhammad , Rabbani Masood , Mushtaq Muhammad Hassan
Published: April 2023

Fowl typhoid, a septicaemic disease of poultry, is caused by Salmonella Gallinarum and leads to severe economic losses. The aim of the present study was to isolate, select and characterize indigenous probiotic lactobacilli with anti-Salmonella Gallinarum activity. A total 55 lactobacilli were isolated from the caeca and ileum parts of healthy chickens and identified to species level by 16S rDNA sequencing. All the isolates were initially screened for antimicrobial activity and selected isolates were further subjected to in vitro evaluation of probiotic properties. Lactobacilli isolates (n = 21) showed varying levels of activity (08–18 mm) against Salmonella Gallinarum. These selected isolates also showed tolerance to acidic conditions (pH 3 and 4). Out of these 21 isolates, 13 showed growth (>0.5 OD at 600 nm) 0.3% bile salts. Moreover, these isolates also had the ability of auto-aggregation (20.05 ± 0.62%–50.70 ± 1.40%), and co-aggregation with Salmonella Gallinarum (5.22 ± 0.21%–42.07 ± 0.70%). Results revealed that lactobacilli had a higher level of resistance to vancomycin (100%), streptomycin (100%), ciprofloxacin (95%), gentamicin (90%), doxycycline (90%), oxytetracycline (85%), and bacitracin (80%), and a lower level of resistance to penicillin (33%), erythromycin (28%), chloramphenicol (23%), fusidic acid (23%) and amoxicillin (4%). The Limosilactobacillus fermentum PC-10 and PC-76 were sensitive to most of the antibiotics. The overall results revealed that two Limosilactobacillus fermentum strains (PC-10 and PC-76) fulfill the in vitro selection criteria of probiotics, i.e, tolerance to low pH, resistance to bile salts, auto-aggregation, co-aggregation with Salmonella Gallinarum, and absence of acquired antibiotic resistance. The Limosilactobacillus fermentum PC-10 and PC-76 also inhibited the (>5 log10) growth of Salmonella Gallinarum in co-culture assay. It is concluded that Limosilactobacillus fermentum PC-10 and PC-76 may be further investigated and developed as anti-Salmonella Gallinarum probiotics for poultry.
Detection of Rickettsia spp. in Animals and Ticks in Midwestern Brazil, Where Human Cases of Rickettsiosis Were Reported
Authors: Neves Lucianne Cardoso , Paula Warley Vieira de Freitas , de Paula Luiza Gabriella Ferreira , da Silva Bianca Barbara Fonseca , Dias Sarah Alves , Pereira Brenda Gomes , Silva Bruno Sérgio Alves , Sevá Anaiá da Paixão , Dantas-Torres Filipe , Labruna Marcelo B. , Krawczak Felipe da Silva
Published: April 2023

Brazilian spotted fever (BSF) is the most important tick-borne diseases affecting humans in Brazil. Cases of BSF have recently been reported in the Goiás state, midwestern Brazil. All cases have been confirmed by reference laboratories by seroconversion to Rickettsia rickettsii antigens. Because serological cross-reactions among different rickettsial species that belong to the spotted fever group (SFG) are common, the agent responsible for BSF cases in Goiás remains unknown. From March 2020 to April 2022, ticks and plasma were collected from dogs, horses and capybaras (Hydrochoerus hydrochaeris), and from the vegetation in an area where BSF cases have been reported and two areas under epidemiological surveillance in Goiás. Horses were infested by Amblyomma sculptum, Dermacentor nitens and Rhipicephalus microplus; dogs by Rhipicephalus sanguineus sensu lato (s.l.), Amblyomma ovale and A. sculptum, and capybaras by A. sculptum and Amblyomma dubitatum. Adults of A. sculptum, A. dubitatum, Amblyomma rotundatum and immature stages of A. sculptum and A. dubitatum, and Amblyomma spp. were collected from the vegetation. DNA of Rickettsia that did not belong to the SFG was detected in A. dubitatum, which was identified by DNA sequencing as Rickettsia bellii. Seroreactivity to SFG and Rickettsia bellii antigens was detected in 25.4% (42/165) of dogs, 22.7% (10/44) of horses and 41.2% (7/17) of capybaras, with higher titers for R. bellii in dogs and capybaras. The seropositivity of animals to SFG Rickettsia spp. antigens demonstrates the circulation of SFG rickettsiae in the region. Further research is needed to fully determine the agent responsible for rickettsiosis cases in this area.
Evaluation of Meat Quality of Local Pigeon Varieties in China
Authors: Chang Lingling , Tang Qingping , Zhang Rui , Fu Shengyong , Mu Chunyu , Shen Xinyue , Bu Zhu
Published: April 2023

To evaluate the germplasm characteristics and nutritional value of Chinese native pigeon varieties, this study analyzed the nutrient composition of the meat of four Chinese native pigeon varieties and then compared them with those of the White King pigeon, which is the most commonly used in China. A total of 150 pigeons aged 28 d (squabs) of 5 breeds including Taihu pigeon, Shiqi pigeon, Ta-rim pigeon, Boot pigeon, and White King pigeon were selected for slaughter. The basic meat quality parameters and contents of conventional nutritional compositions, inosine acid, amino acids, and fatty acids were measured. The results showed that there were significant differences in flesh color (L*, b*), pH, and water loss rate of different breeds of suckling pigeons (p < 0.05). Compared with White King pigeons, four local breeds had dark breast meat and a low water loss rate. The protein contents of Taihu, Tarim, and Shiqi suckling pigeons were significantly higher than those of White King pigeons (p < 0.05). Taihu pigeons had the highest protein content, reaching 22.72%. The inosinic acid content of Tarim pigeons was the highest (1.31 mg/g) and was significantly higher than that of Shiqi pigeons, Boot pigeons, and White King pigeons (p < 0.05). There was no significant difference in the content of amino acids, the ratio of essential amino acids, and the ratio of umami amino acids in the meat of different breeds of pigeons (p > 0.05). The percentage of saturated fatty acids (SFAs) in the breast muscle of local breeding pigeons was significantly lower than that of White King pigeons (p < 0.05), and the percentages of lauric acid, palmitic acid, eicosanoic acid, and behenic acid in SFAs reached significant levels (p < 0.05). The content of eicosapentaenoic acid (EPA) in the meat of Taihu pigeons was significantly higher than that in other breeds. In conclusion, compared with the White King pigeon, the meat of local breed pigeons (Taihu pigeon, Shiqi pigeon, Tarim pigeon, and Boot pigeon) had dark flesh, good water retention, high protein and inosine contents, a high proportion of essential amino acids, and a low saturated fatty acid ratio. In addition, Taihu pigeons had the highest protein content (22.72%), monounsaturated fatty acids (44.58%), and EPA (0.47%) compared to other breeds.
TikTok’s Falco tinnunculus: Getting to Know Urban Wildlife through Social Media
Authors: Yin Duo , Chen Jiachun , Gao Quan
Published: April 2023

Wildlife residing in cities has made encounters between humans and wild animals a common phenomenon. The perspective of the conflict-laden animal–human relationship has been over-emphasized by traditional media, which neglects the peaceful and harmonious daily encounters between residents and urban wildlife. This paper addresses the lacuna in extant literature by examining the virtual encounters between urban residents and wildlife on TikTok by sharing the living habits of Falco tinnunculus. Participatory observation, semi-structured interviews, and text analysis were adopted to explore the knowledge production process of urban wildlife as well as the emotional response of audiences. We found that displaying urban wildlife in short videos is a dynamic process involving the mutual participation of wildlife and humans. Meanwhile, audiences’ anthropocentric gaze of wildlife via TikTok attends to their desires for intimacy with nature and demonstrates the unequal and unbalanced power between wild animals and humans. These findings suggest that more efforts should be made to guide the public to pay attention to native urban wildlife species and to reflect upon the ethics and rationality of such unequal power relations between wild animals and humans.
Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes
Authors: Zeng Fangyuan , Li Lan , Yang Jiaqi , Liu Siqi , Yuan Yang , Zhao Chenxu , Wang Jianguo
Published: April 2023

Strontium (Sr) is a trace element found mainly in bone, and it performs a dual action by promoting bone formation and inhibiting bone resorption. Sr has been used to evaluate the gastrointestinal calcium (Ca) absorption capacity of dairy cows due to the similar physicochemical properties of the two elements. However, the possible effects of Sr on dairy cows remain unclear. This study aimed to explore the potential regulatory mechanism of Sr in bovine chondrocytes by performing transcriptomic and proteomic analyses. A total of 111 genes (52 up-regulated and 59 down-regulated) were identified as significantly altered (1.2-fold change and p < 0.05) between control and Sr-treated groups. Moreover, LC-MS-based proteomic analysis detected 286 changed proteins (159 up-regulated and 127 down-regulated) between the control and Sr-treated groups (1.2-fold change and p < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations of a combination analysis of the transcriptomic and proteomic data revealed that the genes were predominantly involved in chondrocyte proliferation and differentiation, fat metabolism, the inflammation process, and immune responses. Overall, our data reveal a potential regulatory mechanism of strontium in bovine chondrocytes, thus providing further insights into the functions and application of Sr in ruminants.