bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiscale analysis of fine slag from pulverized coal gasification in entrained-flow bed
Authors: Mao Lirui , Zheng Mingdong , Xia Baoliang , Jiao Facun , Liu Tao , Zhang Yuanchun , Gao Shengtao , Li Hanxu
Published: February 2024

Fine slag (FS) is an unavoidable by-product of coal gasification. FS, which is a simple heap of solid waste left in the open air, easily causes environmental pollution and has a low resource utilization rate, thereby restricting the development of energy-saving coal gasification technologies. The multiscale analysis of FS performed in this study indicates typical grain size distribution, composition, crystalline structure, and chemical bonding characteristics. The FS primarily contained inorganic and carbon components (dry bases) and exhibited a "three-peak distribution" of the grain size and regular spheroidal as well as irregular shapes. The irregular particles were mainly adsorbed onto the structure and had a dense distribution and multiple pores and folds. The carbon constituents were primarily amorphous in structure, with a certain degree of order and active sites. C 1s XPS spectrum indicated the presence of C–C and C–H bonds and numerous aromatic structures. The inorganic components, constituting 90% of the total sample, were primarily silicon, aluminum, iron, and calcium. The inorganic components contained Si–O-Si, Si–O–Al, Si–O, SO42−, and Fe–O bonds. Fe 2p XPS spectrum could be deconvoluted into Fe 2p1/2 and Fe 2p3/2 peaks and satellite peaks, while Fe existed mainly in the form of Fe(III). The findings of this study will be beneficial in resource utilization and formation mechanism of fine slag in future.
Soothing the Self-Threat of Idea Theft
Authors: Wheeler-Smith Sara L. , Moulton-Tetlock Edythe E.
Published: February 2024

The creative process has the potential to increase wellbeing and foster human flourishing (Dolan and Metcalfe, 2012; Forgeard and Eichner, 2014; O’Brien and Murray, 2015; Conner et al., 2018; Kaufman, 2018), yet has received little attention in the humanistic management literature. In this paper, we present three experiments showing that idea originators experience greater relationship conflict with counterparts who have committed perceived “idea theft”, i.e., proposed identical or related ideas. We test a model that identifies a mechanism—self-threat—that mediates the relationship between idea theft and relationship conflict and identifies an intervention to ameliorate that self-threat via self-affirmation. Study 1 demonstrates the idea originator’s attribution of malign attributions to, and negative moral emotions toward, the counterpart. Study 2 demonstrates that idea originators ostracize both those who propose identical ideas and those who propose merely related ideas. In Study 3, evidence for self-threat as the mechanism underlying the negative relational consequences is provided via both a direct test of mediation as well as via moderation by self-affirmation. Our research contributes to the nascent idea theft literature and suggests a way to reduce its negative consequences.
The Emerging Concept of the Human-Centered Organization: A Review and Synthesis of the Literature
Authors: Townsend Maya , Romme A. Georges L.
Published: February 2024

Both practitioners and scholars are increasingly interested in the idea of the human-centered organization. This term first appeared in the late 1950s and has gained attention in the last ten years. Awareness of the need for human-centeredness grew during the COVID-19 pandemic, in which many organizational leaders were compelled to focus on employee health, safety, and well-being. In this paper, we review and synthesize the rather fragmented scholarly and practitioner literature on human-centered organization (HCO) to develop an integrated definition and framework. The 26 sources reviewed in depth indicate that the HCO construct is primarily utilized in two ways. First, human-centered design scholars and practitioners conceive of HCOs as employing human-centered design practices. The second discourse involves the humanistic management and culture literature, which conceives of HCOs as embodying humanistic values and cultures. After reviewing these separate discourses, we synthesize them in an integrated definition as well as framework of HCO. The framework starts from humanistic values such as dignity, well-being, and justice, which are pivotal in creating organizational practices characterized by a common good purpose, positive human experiences on the job, team structures to coordinate work, and participatory tools and approaches.
Prognostic factors of poor outcomes in pneumonia in older adults: aspiration or frailty?
Authors: Yoshimatsu Yuki , Thomas Heledd , Thompson Trevor , Smithard David G.
Published: February 2024

Purpose

Little is known about the long-term and functional prognoses of older adults with pneumonia, which complicates their management. There is a common belief that aspiration is a poor prognostic factor; however, the diagnosis of aspiration pneumonia (AP) lacks consensus criteria and is mainly based on clinical characteristics typical of the frailty syndrome. Therefore, the poor prognosis of AP may also be a result of frailty rather than aspiration. This study investigated the impact of AP and other prognostic factors in older patients with pneumonia.

Methods

We performed a retrospective cohort study of patients aged 75 years and older, admitted with pneumonia in 2021. We divided patients according to their initial diagnosis (AP or non-AP), compared outcomes using Kaplan–Meier curves, and used logistic regression to identify independent prognostic factors.

Results

803 patients were included, with a median age of 84 years and 52.7% were male. 17.3% were initially diagnosed with AP. Mortality was significantly higher in those diagnosed with AP than non-AP during admission (27.6% vs 19.0%, p = 0.024) and at 1 year (64.2% vs 53.1%, p = 0.018), with survival analysis showing a median survival time of 62 days and 274 days in AP and non-AP, respectively (χ2 = 9.2, p = 0.002). However, the initial diagnosis of AP was not an independent risk factor for poor prognosis in multivariable analysis. Old age, frailty and cardio-respiratory comorbidities were the main factors associated with death.

Conclusion

The greater mortality in AP may be a result of increased frailty rather than the diagnosis of aspiration itself. This supports our proposal for a paradigm shift from making predictions based on the potentially futile labelling of AP or non-AP, to considering frailty and overall condition of the patient.
Infrastructuring Educational Genomics: Associations, Architectures, and Apparatuses
Authors: Williamson Ben , Kotouza Dimitra , Pickersgill Martyn , Pykett Jessica
Published: February 2024

Technoscientific transformations in molecular genomics have begun to influence knowledge production in education. Interdisciplinary scientific consortia are seeking to identify ‘genetic influences’ on ‘educationally relevant’ traits, behaviors, and outcomes. This article examines the emerging ‘knowledge infrastructure’ of educational genomics, attending to the assembly and choreography of organizational associations, epistemic architecture, and technoscientific apparatuses implicated in the generation of genomic understandings from masses of bioinformation. As an infrastructure of datafied knowledge production, educational genomics is embedded in data-centered epistemologies and practices which recast educational problems in terms of molecular genetic associations—insights about which are deemed discoverable from digital bioinformation and potentially open to genetically informed interventions in policy and practice. While scientists claim to be ‘opening the black box of the genome’ and its association with educational outcomes, we open the black box of educational genomics itself as a source of emerging scientific authority. Data-intensive educational genomics does not straightforwardly ‘discover’ the biological bases of educationally relevant behaviors and outcomes. Rather, this knowledge infrastructure is also an experimental ‘ontological infrastructure’ supporting particular ways of knowing, understanding, explaining, and intervening in education, and recasting the human subjects of education as being surveyable and predictable through the algorithmic processing of bioinformation.
Toward Understanding Polar Heat Transport Enhancement in Subglacial Oceans on Icy Moons
Authors: Hartmann Robert , Stevens Richard J. A. M. , Lohse Detlef , Verzicco Roberto
Published: February 2024

The interior oceans of several icy moons are considered as affected by rotation. Observations suggest a larger heat transport around the poles than at the equator. Rotating Rayleigh‐Bénard convection (RRBC) in planar configuration can show an enhanced heat transport compared to the non‐rotating case within this “rotation‐affected” regime. We investigate the potential for such a (polar) heat transport enhancement in these subglacial oceans by direct numerical simulations of RRBC in spherical geometry for Ra = 106 and 0.7 ≤ Pr ≤ 4.38. We find an enhancement up to 28% in the “polar tangent cylinder,” which is globally compensated by a reduced heat transport at low latitudes. As a result, the polar heat transport can exceed the equatorial by up to 50%. The enhancement is mostly insensitive to different radial gravity profiles, but decreases for thinner shells. In general, polar heat transport and its enhancement in spherical RRBC follow the same principles as in planar RRBC.
Variability of Inflowing Current Into the Dotson Ice Shelf and Its Cause in the Amundsen Sea
Authors: Yang Heewon , Kim Tae‐Wan , Kim Yeonggi , Yoo Jaeill , Park Jisoo , Cho Yang‐Ki
Published: February 2024

The inflow of warm and salty Circumpolar Deep Water affects the melting of the ice shelf on the Amundsen Sea, a significant contributor to global sea level rise. Multi‐year mooring data (2014–2016 and 2018–2020) from the front of the Dotson Ice Shelf show the modified Circumpolar Deep Water layer was thicker during 2018–2020 than during 2014–2016. During 2014–2016, Ocean surface stress curl influenced the barotropic process and strengthened southward velocity, while during 2018–2020, it caused lift and downwelling of thermocline depth, increasing the impact of the baroclinic process in ocean circulation. The heat transport to the ice shelf during 2018–2020 (57.42 MW m−1) was half as much as it was during 2014–2016 (111.06 MW m−1) due to a weaker lower layer current. The difference in ocean circulation between two periods, caused by a difference in warm layer thickness, ultimately impacts the heat transport entering the ice shelf cavity.
Aerosol‐Correlated Cloud Activation for Clean Conditions in the Tropical Atlantic Boundary Layer During LASIC
Authors: Dedrick Jeramy L. , Russell Lynn M. , Sedlacek Arthur J. , Kuang Chongai , Zawadowicz Maria A. , Lubin Dan
Published: February 2024

Aerosol measurements during the DOE ARM Layered Atlantic Smoke Interactions with Clouds (LASIC) campaign were used to quantify the differences between clean and smoky cloud condensation nuclei (CCN) budgets. Accumulation‐mode particles accounted for ∼70% of CCN at supersaturations <0.3% in clean and smoky conditions. Aitken‐mode particles contributed <20% and sea‐spray‐mode particles <10% at supersaturations <0.3%, but at supersaturations >0.3% Aitken particles contributions increased to 30%–40% of clean CCN. For clean conditions, the Hoppel minimum diameter was correlated to the accumulation‐mode number concentration, indicating aerosol‐correlated cloud activation was controlling the lower diameter cutoff for which particles serve as CCN. For smoky conditions, the contributions of Aitken particles increase and the correlation of cloud activation to accumulation‐mode particles is masked by the lower‐hygroscopicity smoke. These results provide the first multi‐month in situ quantitative constraints on the role of aerosol number size distributions in controlling cloud activation in the tropical Atlantic boundary layer.
La Niña's Teleconnection to the Indian Ocean Dipole Controlled by Its Longitudinal Position
Authors: Zhang Teng , Zhang Wenjun , Jiang Feng , Jin Fei‐Fei
Published: February 2024

While the prominent influence of El Niño‐Southern Oscillation (ENSO) on the Indian Ocean Oscillation (IOD) is widely recognized, intricate relationships between them are often invoked that introduce challenges into seasonal predictions. Previous studies have shown that different flavors of El Niño exhibit distinct associations with the IOD. In this study, we demonstrate that La Niña's teleconnection to the IOD is primarily controlled by its longitudinal position. Westward‐displaced La Niña events tend to produce stronger negative convection anomalies in the central Pacific and more pronounced Walk Circulation anomalies, thereby triggering strong negative IOD events. In contrast, eastward‐displaced La Niña events are usually accompanied by feeble convection response due to the excessively cold conditions in the cold tongue, yielding insignificant IOD response. The pivotal role of La Niña's longitudinal position on the IOD's response is realistically reproduced by targeted pacemaker experiments, providing new insights into inter‐basin climate connections.
The Projected Poleward Shift of Tropical Cyclogenesis at a Global Scale Under Climate Change in MRI‐AGCM3.2H
Authors: Cao Xi , Watanabe Masahiro , Wu Renguang , Chen Wen , Sun Ying , Yan Qing , Wang Pengfei , Deng Difei , Wu Liang
Published: February 2024

Future climate projections suggest a poleward shift of the maximum intensity of tropical cyclones (TCs) over the western North Pacific. However, the global nature of the latitudinal change in TC genesis under global warming remains poorly understood. We show, using large‐ensemble high‐resolution atmospheric model simulations (d4PDF) with four warming scenarios, that the poleward shift is a robust change over the globe, attributable to the weakening of the Hadley circulation. The weakened ascent driven by the upper‐tropospheric warming suppresses the TC genesis within 5°–20° latitudes, whereas the weakened descent enhances the TC genesis in the poleward latitudes. We further estimate the poleward shift of TC genesis to emerge at the 2 K global warming over the Arabian Sea, South Atlantic and Pacific Oceans and at the 4 K warming over the North Pacific. The present results underscore the potential for increasing social and economic risks associated with TCs at higher latitudes.
Arctic Sea Ice Loss Modulates the Surface Impact of Autumn Stratospheric Polar Vortex Stretching Events
Authors: Zou Chuntao , Zhang Ruonan
Published: February 2024

The cold Eurasia has been proposed to be closely linked to the weakening of the stratospheric polar vortex (SPV), however, how the Arctic sea ice modulates the surface impacts of the weak SPV is unclear. This study explores the critical modulating role of reduced Arctic sea ice in the surface cooling response to SPV stretching events in autumn. Here, through ERA5 reanalysis and Whole Atmosphere Community Climate Model simulations, we show that Eurasian cold events are more likely (45%) to occur in days 30–50 after the onset of SPV stretching events under lower Barents‐Kara Seas (BKS) sea ice conditions, in contrast to under heavy BKS sea ice conditions when robust surface cooling is absent. The stratospheric and tropospheric pathways explain 46.8% and 53.2% of the total variance of Siberian coldness, respectively. The downward extension of anomalous stratospheric wave‐2 ridge to the troposphere intensifies the Arctic‐North European high, favoring the subsequent colder Siberia.
High Water Levels Cause Problems for Mississippi Shipping
Authors: Banks Shannon
Published: February 2024

Source: Geophysical Research LettersDroughts’ effect on the navigability of rivers is well documented: During the summer and fall of 2022,.
Antibodies toward Na+,HCO3–-cotransporter NBCn1/SLC4A7 block net acid extrusion and cause pH-dependent growth inhibition and apoptosis in breast cancer
Authors: Axelsen Trine V. , Olesen Claus , Khan Danish , Mohammadi Ali , Bouzinova Elena V. , Nielsen Christine J. F. , Mele Marco , Hauerslev Katrine R. , Pedersen Helene L. , Balling Eva , Vahl Pernille , Tramm Trine , Christiansen Peer M. , Boedtkjer Ebbe
Published: February 2024

Background

Na+,HCO3–-cotransporter NBCn1/Slc4a7 accelerates murine breast carcinogenesis. Lack of specific pharmacological tools previously restricted therapeutic targeting of NBCn1 and identification of NBCn1-dependent functions in human breast cancer.

Methods

We develop extracellularly-targeted anti-NBCn1 antibodies, screen for functional activity on cells, and evaluate (a) mechanisms of intracellular pH regulation in human primary breast carcinomas, (b) proliferation, cell death, and tumor growth consequences of NBCn1 in triple-negative breast cancer, and (c) association of NBCn1-mediated Na+,HCO3–-cotransport with human breast cancer metastasis.

Results

We identify high-affinity (KD ≈ 0.14 nM) anti-NBCn1 antibodies that block human NBCn1-mediated Na+,HCO3–-cotransport in cells, without cross-reactivity towards human NBCe1 or murine NBCn1. These anti-NBCn1 antibodies abolish Na+,HCO3–-cotransport activity in freshly isolated primary organoids from human breast carcinomas and lower net acid extrusion effectively in primary breast cancer tissue from patients with macrometastases in axillary lymph nodes. Inhibitory anti-NBCn1 antibodies decelerate tumor growth in vivo by ~50% in a patient-derived xenograft model of triple-negative breast cancer and pH-dependently reduce colony formation, cause G2/M-phase cell cycle accumulation, and increase apoptosis of metastatic triple-negative breast cancer cells in vitro.

Conclusions

Inhibitory anti-NBCn1 antibodies block net acid extrusion in human breast cancer tissue, particularly from patients with disseminated disease, and pH-dependently limit triple-negative breast cancer growth.
Validation of WINROP algorithm as screening tool of retinopathy of prematurity among Egyptian preterm neonates
Authors: Fares Asmaa , Abdelmonaim Sherif , Sayed Dina , Sadek Sherin , Abdulrazek Abdulrahman , Helmy Youssef , Maher Sara
Published: February 2024

Background

Retinopathy of prematurity (ROP) is a leading cause of preventable childhood blindness worldwide. Proper screening for ROP can prevent loss of vision. WINROP (weight, insulin-like growth factor 1, neonatal, retinopathy of prematurity) is an online surveillance system based on gestational age, birth weight and weekly weight gain that can predict infants at risk of sight-threatening retinopathy of prematurity.

Aims

To evaluate the diagnostic accuracy of WINROP algorithm in detecting sight-threatening ROP in Egyptian preterm neonates.

Methods

Birth weight (BW), gestational age (GA) and weekly weight measurement of 365 preterm infants were prospectively entered into WINROP algorithm. Based on these inputs, the algorithm would output and a screening was performed as is standard. Sensitivity, specificity, and predictive values were calculated by comparing WINROP outcomes with ROP screening outcomes.

Results

Of the infants included in the study the mean GA was ±31.24 and mean BW was ±1508.78. A high risk WINROP alarm was triggered in 62 infants of whom 16 infants develop type 1 or type 2 ROP. These infants had associated comorbidities including sepsis, Intraventricular hemorrhage (IVH), Necrotizing enterocolitis (NEC), history of transfusion of packed red blood cells (RBCS) and history of platelet transfusion. A low risk WINROP alarm was triggered in 303 infants of whom 15 infants developed type 1 or type 2ROP. WINROP showed a sensitivity of 51.6%, a specificity of 86.2%, a positive predictive value (PPV) of 52.8% and a negative predictive value (NPV) of 95% for detection of type 1 or type 2 ROP.

Conclusion

WINROP has low sensitivity and high specificity for detection of ROP. It may help in ROP prediction but can’t be used alone. Modification of WINROP algorithm taking into account other risk factors may improve sensitivity and reduce number for ROP examination.
5-aminosalicylic acid suppresses osteoarthritis through the OSCAR-PPARγ axis
Authors: Kim Jihee , Ryu Gina , Seo Jeongmin , Go Miyeon , Kim Gyungmin , Yi Sol , Kim Suwon , Lee Hana , Lee June-Yong , Kim Han Sung , Park Min-Chan , Shin Dong Hae , Shim Hyunbo , Kim Wankyu , Lee Soo Young
Published: February 2024

Osteoarthritis (OA) is a progressive and irreversible degenerative joint disease that is characterized by cartilage destruction, osteophyte formation, subchondral bone remodeling, and synovitis. Despite affecting millions of patients, effective and safe disease-modifying osteoarthritis drugs are lacking. Here we reveal an unexpected role for the small molecule 5-aminosalicylic acid (5-ASA), which is used as an anti-inflammatory drug in ulcerative colitis. We show that 5-ASA competes with extracellular-matrix collagen-II to bind to osteoclast-associated receptor (OSCAR) on chondrocytes. Intra-articular 5-ASA injections ameliorate OA generated by surgery-induced medial-meniscus destabilization in male mice. Significantly, this effect is also observed when 5-ASA was administered well after OA onset. Moreover, mice with DMM-induced OA that are treated with 5-ASA at weeks 8–11 and sacrificed at week 12 have thicker cartilage than untreated mice that were sacrificed at week 8. Mechanistically, 5-ASA reverses OSCAR-mediated transcriptional repression of PPARγ in articular chondrocytes, thereby suppressing COX-2-related inflammation. It also improves chondrogenesis, strongly downregulates ECM catabolism, and promotes ECM anabolism. Our results suggest that 5-ASA could serve as a DMOAD.
Room-temperature sub-100 nm Néel-type skyrmions in non-stoichiometric van der Waals ferromagnet Fe3-xGaTe2 with ultrafast laser writability
Authors: Li Zefang , Zhang Huai , Li Guanqi , Guo Jiangteng , Wang Qingping , Deng Ying , Hu Yue , Hu Xuange , Liu Can , Qin Minghui , Shen Xi , Yu Richeng , Gao Xingsen , Liao Zhimin , Liu Junming , Hou Zhipeng , Zhu Yimei , Fu Xuewen
Published: February 2024

Realizing room-temperature magnetic skyrmions in two-dimensional van der Waals ferromagnets offers unparalleled prospects for future spintronic applications. However, due to the intrinsic spin fluctuations that suppress atomic long-range magnetic order and the inherent inversion crystal symmetry that excludes the presence of the Dzyaloshinskii-Moriya interaction, achieving room-temperature skyrmions in 2D magnets remains a formidable challenge. In this study, we target room-temperature 2D magnet Fe3GaTe2 and unveil that the introduction of iron-deficient into this compound enables spatial inversion symmetry breaking, thus inducing a significant Dzyaloshinskii-Moriya interaction that brings about room-temperature Néel-type skyrmions with unprecedentedly small size. To further enhance the practical applications of this finding, we employ a homemade in-situ optical Lorentz transmission electron microscopy to demonstrate ultrafast writing of skyrmions in Fe3-xGaTe2 using a single femtosecond laser pulse. Our results manifest the Fe3-xGaTe2 as a promising building block for realizing skyrmion-based magneto-optical functionalities.
Acoustic spin rotation in heavy-metal-ferromagnet bilayers
Authors: Cao Yang , Ding Hao , Zuo Yalu , Li Xiling , Zhao Yibing , Li Tong , Lei Na , Cao Jiangwei , Si Mingsu , Xi Li , Jia Chenglong , Xue Desheng , Yang Dezheng
Published: February 2024

Through pumping a spin current from ferromagnet into heavy metal (HM) via magnetization precession, parts of the injected spins are in-plane rotated by the lattice vibration, namely acoustic spin rotation (ASR), which manifests itself as an inverse spin Hall voltage in HM with an additional 90° difference in angular dependency. When reversing the stacking order of bilayer with a counter-propagating spin current or using HMs with an opposite spin Hall angle, such ASR voltage shows the same sign, strongly suggesting that ASR changes the rotation direction due to interface spin-orbit interaction. With the drift-diffusion model of spin transport, we quantify the efficiency of ASR up to 30%. The finding of ASR endows the acoustic device with an ability to manipulate spin, and further reveals a new spin-orbit coupling between spin current and lattice vibration.
Lattice oxygen activation and local electric field enhancement by co-doping Fe and F in CoO nanoneedle arrays for industrial electrocatalytic water oxidation
Authors: Ye Pengcheng , Fang Keqing , Wang Haiyan , Wang Yahao , Huang Hao , Mo Chenbin , Ning Jiqiang , Hu Yong
Published: February 2024

Oxygen evolution reaction (OER) is critical to renewable energy conversion technologies, but the structure-activity relationships and underlying catalytic mechanisms in catalysts are not fully understood. We herein demonstrate a strategy to promote OER with simultaneously achieved lattice oxygen activation and enhanced local electric field by dual doping of cations and anions. Rough arrays of Fe and F co-doped CoO nanoneedles are constructed, and a low overpotential of 277 mV at 500 mA cm−2 is achieved. The dually doped Fe and F could cooperatively tailor the electronic properties of CoO, leading to improved metal-oxygen covalency and stimulated lattice oxygen activation. Particularly, Fe doping induces a synergetic effect of tip enhancement and proximity effect, which effectively concentrates OH− ions, optimizes reaction energy barrier and promotes O2 desorption. This work demonstrates a conceptual strategy to couple lattice oxygen and local electric field for effective electrocatalytic water oxidation.
Unravelling the amorphous structure and crystallization mechanism of GeTe phase change memory materials
Authors: Wintersteller Simon , Yarema Olesya , Kumaar Dhananjeya , Schenk Florian M. , Safonova Olga V. , Abdala Paula M. , Wood Vanessa , Yarema Maksym
Published: February 2024

The reversible phase transitions in phase-change memory devices can switch on the order of nanoseconds, suggesting a close structural resemblance between the amorphous and crystalline phases. Despite this, the link between crystalline and amorphous tellurides is not fully understood nor quantified. Here we use in-situ high-temperature x-ray absorption spectroscopy (XAS) and theoretical calculations to quantify the amorphous structure of bulk and nanoscale GeTe. Based on XAS experiments, we develop a theoretical model of the amorphous GeTe structure, consisting of a disordered fcc-type Te sublattice and randomly arranged chains of Ge atoms in a tetrahedral coordination. Strikingly, our intuitive and scalable model provides an accurate description of the structural dynamics in phase-change memory materials, observed experimentally. Specifically, we present a detailed crystallization mechanism through the formation of an intermediate, partially stable ‘ideal glass’ state and demonstrate differences between bulk and nanoscale GeTe leading to size-dependent crystallization temperature.
Charge-density wave mediated quasi-one-dimensional Kondo lattice in stripe-phase monolayer 1T-NbSe2
Authors: Liu Zhen-Yu , Jin Heng , Zhang Yao , Fan Kai , Guo Ting-Fei , Qin Hao-Jun , Zhu Lan-Fang , Yang Lian-Zhi , Zhang Wen-Hao , Huang Bing , Fu Ying-Shuang
Published: February 2024

The heavy fermion physics is dictated by subtle competing exchange interactions, posing a challenge to their understanding. One-dimensional (1D) Kondo lattice model has attracted special attention in theory, because of its exact solvability and expected unusual quantum criticality. However, such experimental material systems are extremely rare. Here, we demonstrate the realization of quasi-1D Kondo lattice behavior in a monolayer van der Waals crystal NbSe2, that is driven into a stripe phase via Se-deficient line defects. Spectroscopic imaging scanning tunneling microscopy measurements and first-principles calculations indicate that the stripe-phase NbSe2 undergoes a novel charge-density wave transition, creating a matrix of local magnetic moments. The Kondo lattice behavior is manifested as a Fano resonance at the Fermi energy that prevails the entire film with a high Kondo temperature. Importantly, coherent Kondo screening occurs only in the direction of the stripes. Upon approaching defects, the Fano resonance exhibits prominent spatial 1D oscillations along the stripe direction, reminiscent of Kondo holes in a quasi-1D Kondo lattice. Our findings provide a platform for exploring anisotropic Kondo lattice behavior in the monolayer limit.
Gestational diabetes augments group B Streptococcus infection by disrupting maternal immunity and the vaginal microbiota
Authors: Mercado-Evans Vicki , Mejia Marlyd E. , Zulk Jacob J. , Ottinger Samantha , Hameed Zainab A. , Serchejian Camille , Marunde Madelynn G. , Robertson Clare M. , Ballard Mallory B. , Ruano Simone H. , Korotkova Natalia , Flores Anthony R. , Pennington Kathleen A. , Patras Kathryn A.
Published: February 2024

Group B Streptococcus (GBS) is a pervasive perinatal pathogen, yet factors driving GBS dissemination in utero are poorly defined. Gestational diabetes mellitus (GDM), a complication marked by dysregulated immunity and maternal microbial dysbiosis, increases risk for GBS perinatal disease. Using a murine GDM model of GBS colonization and perinatal transmission, we find that GDM mice display greater GBS in utero dissemination and subsequently worse neonatal outcomes. Dual-RNA sequencing reveals differential GBS adaptation to the GDM reproductive tract, including a putative glycosyltransferase (yfhO), and altered host responses. GDM immune disruptions include reduced uterine natural killer cell activation, impaired recruitment to placentae, and altered maternofetal cytokines. Lastly, we observe distinct vaginal microbial taxa associated with GDM status and GBS invasive disease status. Here, we show a model of GBS dissemination in GDM hosts that recapitulates several clinical aspects and identifies multiple host and bacterial drivers of GBS perinatal disease.
Etrolizumab-s fails to control E-Cadherin-dependent co-stimulation of highly activated cytotoxic T cells
Authors: Wiendl Maximilian , Dedden Mark , Liu Li-Juan , Schweda Anna , Paap Eva-Maria , Ullrich Karen A.-M. , Hartmann Leonie , Wieser Luisa , Vitali Francesco , Atreya Imke , Müller Tanja M. , Günther Claudia , Atreya Raja , Neurath Markus F. , Zundler Sebastian
Published: February 2024

Despite promising preclinical and earlier clinical data, a recent phase III trial on the anti-β7 integrin antibody etrolizumab in Crohn’s disease (CD) did not reach its primary endpoint. The mechanisms leading to this outcome are not well understood. Here we characterize the β7+ T cell compartment from patients with CD in comparison to cells from individuals without inflammatory bowel disease. By flow cytometric, transcriptomic and functional profiling of circulating T cells, we find that triple-integrin-expressing (α4+β7+β1hi) T cells have the potential to home to the gut despite α4β7 blockade and have a specific cytotoxic signature. A subset of triple-integrin-expressing cells readily acquires αE expression and could be co-stimulated via E-Cadherin-αEβ7 interactions in vitro. Etrolizumab-s fails to block such αEβ7 signalling at high levels of T cell stimulation. Consistently, in CD patients treated with etrolizumab, T cell activation correlates with cytotoxic signatures. Collectively, our findings might add one important piece to the puzzle to explain phase III trial results with etrolizumab, while they also highlight that αEβ7 remains an interesting target for future therapeutic approaches in inflammatory bowel disease.
Orientation-invariant autoencoders learn robust representations for shape profiling of cells and organelles
Authors: Burgess James , Nirschl Jeffrey J. , Zanellati Maria-Clara , Lozano Alejandro , Cohen Sarah , Yeung-Levy Serena
Published: February 2024

Cell and organelle shape are driven by diverse genetic and environmental factors and thus accurate quantification of cellular morphology is essential to experimental cell biology. Autoencoders are a popular tool for unsupervised biological image analysis because they learn a low-dimensional representation that maps images to feature vectors to generate a semantically meaningful embedding space of morphological variation. The learned feature vectors can also be used for clustering, dimensionality reduction, outlier detection, and supervised learning problems. Shape properties do not change with orientation, and thus we argue that representation learning methods should encode this orientation invariance. We show that conventional autoencoders are sensitive to orientation, which can lead to suboptimal performance on downstream tasks. To address this, we develop O2-variational autoencoder (O2-VAE), an unsupervised method that learns robust, orientation-invariant representations. We use O2-VAE to discover morphology subgroups in segmented cells and mitochondria, detect outlier cells, and rapidly characterise cellular shape and texture in large datasets, including in a newly generated synthetic benchmark.
Tigerfish designs oligonucleotide-based in situ hybridization probes targeting intervals of highly repetitive DNA at the scale of genomes
Authors: Aguilar Robin , Camplisson Conor K. , Lin Qiaoyi , Miga Karen H. , Noble William S. , Beliveau Brian J.
Published: February 2024

Fluorescent in situ hybridization (FISH) is a powerful method for the targeted visualization of nucleic acids in their native contexts. Recent technological advances have leveraged computationally designed oligonucleotide (oligo) probes to interrogate > 100 distinct targets in the same sample, pushing the boundaries of FISH-based assays. However, even in the most highly multiplexed experiments, repetitive DNA regions are typically not included as targets, as the computational design of specific probes against such regions presents significant technical challenges. Consequently, many open questions remain about the organization and function of highly repetitive sequences. Here, we introduce Tigerfish, a software tool for the genome-scale design of oligo probes against repetitive DNA intervals. We showcase Tigerfish by designing a panel of 24 interval-specific repeat probes specific to each of the 24 human chromosomes and imaging this panel on metaphase spreads and in interphase nuclei. Tigerfish extends the powerful toolkit of oligo-based FISH to highly repetitive DNA.
Printed smart devices for anti-counterfeiting allowing precise identification with household equipment
Authors: Zhang Junfang , Tan Rong , Liu Yuxin , Albino Matteo , Zhang Weinan , Stevens Molly M. , Loeffler Felix F.
Published: February 2024

Counterfeiting has become a serious global problem, causing worldwide losses and disrupting the normal order of society. Physical unclonable functions are promising hardware-based cryptographic primitives, especially those generated by chemical processes showing a massive challenge-response pair space. However, current chemical-based physical unclonable function devices typically require complex fabrication processes or sophisticated characterization methods with only binary (bit) keys, limiting their practical applications and security properties. Here, we report a flexible laser printing method to synthesize unclonable electronics with high randomness, uniqueness, and repeatability. Hexadecimal resistive keys and binary optical keys can be obtained by the challenge with an ohmmeter and an optical microscope. These readout methods not only make the identification process available to general end users without professional expertise, but also guarantee device complexity and data capacity. An adopted open-source deep learning model guarantees precise identification with high reliability. The electrodes and connection wires are directly printed during laser writing, which allows electronics with different structures to be realized through free design. Meanwhile, the electronics exhibit excellent mechanical and thermal stability. The high physical unclonable function performance and the widely accessible readout methods, together with the flexibility and stability, make this synthesis strategy extremely attractive for practical applications.
Magnetotransport of Sm2Ir2O7 across the pressure-induced quantum-critical phase boundary
Authors: Coak M. J. , Götze K. , Northam De La Fuente T. , Castelnovo C. , Tidey J. P. , Singleton J. , Boothroyd A. T. , Prabhakaran D. , Goddard P. A.
Published: February 2024

Rare-earth pyrochlore iridates host two interlocking magnetic sublattices of corner-sharing tetrahedra and can harbour a unique combination of frustrated moments, exotic excitations and highly correlated electrons. They are also the first systems predicted to display both topological Weyl semimetal and axion insulator phases. We have measured the transport and magnetotransport properties of single-crystal Sm2Ir2O7 up to and beyond the pressure-induced quantum critical point for all-in-all-out (AIAO) Ir order at pc = 63 kbar previously identified by resonant X-ray scattering and close to which Weyl semimetallic behavior has been previously predicted. Our findings overturn the accepted expectation that the suppression of AIAO order should lead to metallic conduction persisting down to zero temperature. Instead, the resistivity-minimum temperature, which tracks the decrease in the AIAO ordering temperature for pressures up to 30 kbar, begins to increase under further application of pressure, pointing to the presence of a second as-yet unidentified mechanism leading to non-metallic behavior. The magnetotransport does track the suppression of Ir magnetism, however, with a strong hysteresis observed only within the AIAO phase boundary, similar to that found for Ho2Ir2O7 and attributed to plastic deformation of Ir domains. Around pc we find the emergence of a new type of electronic phase, characterized by a negative magnetoresistance with small hysteresis at the lowest temperatures, and hysteresis-free positive magnetoresistance above approximately 5 K. The temperature dependence of our low-temperature transport data are found to be best described by a model consistent with a Weyl semimetal across the entire pressure range.
Chromosome-level genome assembly of Acrossocheilus fasciatus using PacBio sequencing and Hi-C technology
Authors: Zheng Jianbo , Jiang Jianhu , Rui Qianlong , Li Fei , Liu Shili , Cheng Shun , Chi Meili , Jiang Wenping
Published: February 2024

Acrossocheilus fasciatus (Cypriniformes, Cyprinidae) is emerged as a newly commercial stream fish in the south of China with high economic and ornamental value. In this study, a chromosome-level reference genome of A. fasciatus was assembled using PacBio, Illumina and Hi-C sequencing technologies. As a result, a high-quality genome was generated with a size of 879.52 Mb (accession number: JAVLVS000000000), scaffold N50 of 32.7 Mb, and contig N50 of 32.7 Mb. The largest and smallest scafford was 60.57 Mb and 16 kb, respectively. BUSCO analysis showed a completeness score of 98.3%. Meanwhile, the assembled sequences were anchored to 25 pseudo-chromosomes with an integration efficiency of 96.95%. Additionally, we found approximately 390.91 Mb of repetitive sequences that accounting for 44.45% of the assembled genome, and predicted 24,900 protein-coding genes. The available genome reported in the present study provided a crucial resource to further investigate the regulation mechanism of genetic diversity, sexual dimorphism and evolutionary histories.
ArcTiCA: Arctic tidal constituents atlas
Authors: Hart-Davis M. G. , Howard S. L. , Ray R. D. , Andersen O. B. , Padman L. , Nilsen F. , Dettmering D.
Published: February 2024

Tides in the Arctic Ocean affect ocean circulation and mixing, and sea ice dynamics and thermodynamics. However, there is a limited network of available in situ tidal coefficient data for understanding tidal variability in the Arctic Ocean; e.g., the global TICON-3 database contains only 111 sites above 60°N and 21 above 70°N. At the same time, the presence of sea ice and latitude limits of satellite altimetry complicate altimetry-based retrievals of Arctic tidal coefficients. This leads to a reliance on ocean tide models whose accuracy depend on having sufficient in situ data for validation and assimilation. Here, we present a comprehensive new dataset of tidal constituents in the Arctic region, combining analyses of in situ measurements from tide gauges, ocean bottom pressure sensors and GNSS interferometric reflectometry. The new dataset contains 914 measurement sites above 60°N and 399 above 70°N, with each site being quality-assessed and expert guidance provided to help maximise the usage of the dataset. We also compare the dataset to recent tide models.
Ultra-restrictive red blood cell transfusion strategies in extensively burned patients
Authors: Wang Yiran , Zhu Zhikang , Duan Deqing , Xu Wanting , Chen Zexin , Shen Tao , Wang Xingang , Xu Qinglian , Zhang Hongyan , Han Chunmao
Published: February 2024

In recent years, due to the shortage of blood products, some extensive burn patients were forced to adopt an “ultra-restrictive” transfusion strategy, in which the hemoglobin levels of RBC transfusion thresholds were < 7 g/dl or even < 6 g/dl. This study investigated the prognostic impacts of ultra-restrictive RBC transfusion in extensive burn patients. This retrospective multicenter cohort study recruited extensive burns (total body surface area ≥ 50%) from three hospitals in Eastern China between 1 January 2016 and 30 June 2022. Patients were divided into an ultra-restrictive transfusion group and a restrictive transfusion group depending on whether they received timely RBC transfusion at a hemoglobin level < 7 g/dl. 1:1 ratio propensity score matching (PSM) was performed to balance selection bias. Modified Poisson regression and linear regression were conducted for sensitive analysis. Subsequently, according to whether they received timely RBC transfusion at a hemoglobin level < 6 g/dl, patients in the ultra-restrictive transfusion group were divided into < 6 g/dl group and 6–7 g/dl group to further compare the prognostic outcomes. 271 eligible patients with extensive burns were included, of whom 107 patients were in the ultra-restrictive transfusion group and 164 patients were in the restrictive transfusion group. The ultra-restrictive transfusion group had a significantly lower RBC transfusion volume than the restrictive transfusion group (11.5 [5.5, 21.5] vs 17.3 [9.0, 32.5] units, p = 0.004). There were no significant differences between the two groups in terms of in-hospital mortality, risk of infection, hospital length of stay, and wound healing time after PSM or multivariate adjustment (p > 0.05). Among the ultra-restrictive transfusion group, patients with RBC transfusion threshold < 6 g/dl had a significantly higher hospital mortality than 6–7 g/dl (53.1% vs 21.3%, p = 0.001). For extensive burn patients, no significant adverse effects of ultra-restrictive RBC transfusion were found in this study. When the blood supply is tight, it is acceptable to adopt an RBC transfusion threshold of < 7 g/dL but not < 6 g/dL.
Potential anthelmintic effect of chitosan on Syphacia muris infecting Wistar rats: biochemical, immunological, and histopathological studies
Authors: Mostafa Nesma A. , Hamdi Salwa A. H. , Fol Mona F.
Published: February 2024

Natural products extracted from animal sources have many biological activities, such as chitosan, which is being researched for its medicinal or therapeutic potential. Syphacia muris is the most well-known intestinal nematode, infecting laboratory rats and influencing their immune systems. In this study, we looked at the anthelminthic activity of chitosan particles against S. muris infection using biochemical, immunological, and histopathological methods. Chitosan particles were characterized using Fourier-transform infrared spectroscopy (FTIR). Rats were separated into four groups, each consisting of seven individuals (n = 7). The first group was the control (non-infected), the second group was infected, and both groups received 0.5 ml of 1% glacial acetic acid orally. The third group was the infected group (treated), and the fourth group (normal) received 0.5 ml of 30 mg/kg/day chitosan dissolved in 1% glacial acetic acid for 14 days using gavage. Liver and kidney parameters, oxidative stress markers, serum levels of cytokines (IFN-γ, IL-5, IL-13, IL-33, and IL-10), as well as immunoglobulins (total IgE and IgG), were assessed. Histological examinations of host tissues (intestine, liver, kidney, and spleen) were also performed. Following chitosan treatment, a significant decrease in worm count (P < 0.05) was indicated; this was associated with an enhancement of biochemical and oxidative stress biomarkers, which were altered due to infection. Moreover, immunological analysis revealed a significant drop in INF-γ, IL-5, IL-13, and IL-33 levels and total immunoglobulins (IgE and IgG) as well as an improvement in rat tissues. Conclusively, this study showed the anthelminthic effect of chitosan against S. muris infection.
AI models for automated segmentation of engineered polycystic kidney tubules
Authors: Monaco Simone , Bussola Nicole , Buttò Sara , Sona Diego , Giobergia Flavio , Jurman Giuseppe , Xinaris Christodoulos , Apiletti Daniele
Published: February 2024

Autosomal dominant polycystic kidney disease (ADPKD) is a monogenic, rare disease, characterized by the formation of multiple cysts that grow out of the renal tubules. Despite intensive attempts to develop new drugs or repurpose existing ones, there is currently no definitive cure for ADPKD. This is primarily due to the complex and variable pathogenesis of the disease and the lack of models that can faithfully reproduce the human phenotype. Therefore, the development of models that allow automated detection of cysts’ growth directly on human kidney tissue is a crucial step in the search for efficient therapeutic solutions. Artificial Intelligence methods, and deep learning algorithms in particular, can provide powerful and effective solutions to such tasks, and indeed various architectures have been proposed in the literature in recent years. Here, we comparatively review state-of-the-art deep learning segmentation models, using as a testbed a set of sequential RGB immunofluorescence images from 4 in vitro experiments with 32 engineered polycystic kidney tubules. To gain a deeper understanding of the detection process, we implemented both pixel-wise and cyst-wise performance metrics to evaluate the algorithms. Overall, two models stand out as the best performing, namely UNet++ and UACANet: the latter uses a self-attention mechanism introducing some explainability aspects that can be further exploited in future developments, thus making it the most promising algorithm to build upon towards a more refined cyst-detection platform. UACANet model achieves a cyst-wise Intersection over Union of 0.83, 0.91 for Recall, and 0.92 for Precision when applied to detect large-size cysts. On all-size cysts, UACANet averages at 0.624 pixel-wise Intersection over Union. The code to reproduce all results is freely available in a public GitHub repository.