bluuebunny/crossref_metadata_2025 huggingface dataset:
Kajian Pustaka: Intususepsi Usus Kecil dan Usus Besar pada Anjing dan Upaya Penanganannya dengan Pembedahan
Authors: Abrori Muh Muazdzamzam Lil , Pemayun Tjokorda Istri Agung Pradnya Dewi , Putri Rista Ranggalan , Pramita Kadek Lia , Medha Putu Kreshna , Ariandoko Ariandoko , Batan I Wayan
Published: November 2024

Intususepsi adalah perpindahan satu segmen saluran gastrointestinal ke bagian lain, biasanya ke aboral. Kejadian kasus intususepsi ini sebagian besar terjadi pada anjing muda yang bersifat idiopatik, kejadian tersebut biasanya disebabkan oleh infeksi parasit usus, benda asing, dan enteritis akibat infeksi virus. Tanda dan gejala klinis biasanya berbeda-beda antar individu, namun umumnya gejala intususepsi yang pernah dilaporkan adalah muntah, diare berdarah, dan nyeri pada bagian abdomen. Dalam menegakkan diagnosis intususepsi usus selain anamnesis dan pemeriksaan fisik, perlu didukung juga dengan melakukan pemeriksaan penunjang seperti radiografi, ultrasonografi, dan CT. Hasil radoigrafi umumnya menunjukkan adanya temuan intestinal loops yang dekat dengan obstruksi usus terisi oleh udara (gas) ataupun cairan. Hasil USG abdomen umumnya menunjukkan adanya temuan cincin konsentris (multiple concentric rings sign) pada potongan transerval dan beberapa garis paralel hyperechoid dan hypoechoic pada potongan longitudinal. Sementara, hasil CT yang tegak lurus terhadap intususepsi menunjukkan massa pada target dengan area hipodensitas yang terletak secara eksentrik dan diselingi garis hiperdensitas dan hipodensitas di dalam intususepsi. Prognosis intususepsi usus pada kasus yang tidak rumit adalah baik (fausta). Penanganan intususepsi umumnya dilakukan dengan laparatomi. Teknik laparatomi yang digunakan yaitu reduksi manual atau reseksi usus dan anastomosis. Penanganan pascaoperasi dapat diberikan antibiotk, antiemetik, ataupun analgesik opioid untuk mengontrol nyeri.
Effect of Cryopreservation Duration Time on Post-Thawing Sperms’ Characteristics of Goat Semen
Authors: Oktanella Yudit , Mustofa Imam , Susilowati Suherni , Hendrawan Viski Fitri , Hernawan Tatik
Published: November 2024

Cryopreservation is a technique utilized to store spermatozoa with the aim of preserving the quality of frozen semen. The quality of spermatozoa after thawing is a critical factor influencing the success of Artificial Insemination (AI). This decline in quality can be attributed to factors such as exposure to extreme temperatures, formation of ice crystals, reduced membrane permeability and the generation of Reactive Oxygen Species (ROS). Given this decline in quality, it becomes imperative to assess spermatozoa quality at the cellular level. This study was aimed to investigate the impact of storage time on frozen goat semen on spermatozoa membrane integrity, levels of Malondialdehyde (MDA), and sperm DNA fragmentation. The samples utilized included frozen goat semen stored for less than two years and six years, subjected to a thawing process at 37°C for 20 seconds. Data pertaining to the percentage of spermatozoa with intact membrane integrity, sperm DNA fragmentation, and MDA levels (nmol/L) were analyzed using a paired t-test with a 95% confidence level (? = 0.05). The findings of this study revealed that the duration of cryopreservation may not significantly impact post-thawing DNA fragmentation and MDA levels (p>0.05). However, a moderate decrease in sperm viability and intact plasma membrane (IPM) was observed with increasing cryopreservation duration (p<0.05). Furthermore, the IPM parameter emerged as a potentially suitable indicator for assessing the quality of post-thawing frozen semen, alongside sperm viability and motility.
Efek Preventif Bayam Merah terhadap Organ Reproduksi Tikus Wistar Jantan yang Diinduksi Paracetamol Dosis Toksik
Authors: Setiawan Haris , Yunianto Irfan , Tejosukmono Ario , Cahyani Cucu , Sakinati Isma
Published: November 2024

Penggunaan parasetamol dalam dosis berlebih (dosis toksik) dapat membentuk senyawa NAPQI yang menyebabkan peningkatan radikal bebas di dalam tubuh dan berpotensi menurunkan kualitas organ reproduksi. Bayam Merah (Amaranthus tricolor L.) merupakan salah satu tanaman herbal yang berpotensi sebagai sumber antioksidan dalam menangkal radikal bebas akibat parasetamol. Penelitian bertujuan mengetahui potensi ekstrak etanol Bayam Merah terhadap organ reproduksi (kualitas sperma dan histologi testis) Tikus Wistar yang diinduksi parasetamol dosis toksik. Metode penelitian menggunakan 24 ekor Tikus Wistar (4 perlakuan dengan 6 ulangan) yang terdiri dari Kontrol (akuades), KN (parasetamol dosis 0,5 g/kg BB), P1 (ekstrak dosis 0,4 g/kgBB dan parasetamol dosis 0,5 g/kg BB), P2 (ekstrak dosis 0,8 g/kgBB dan parasetamol dosis 0,5 g/kg BB). Parameter organ reproduksi terdiri dari kualitas sperma dan struktur histologi testis (jumlah sel Leydig dan indeks spermatogenesis). Pemberian ekstrak dilakukan pada hari ke 1-15, sedangkan parasetamol diberikan pada hari ke 11-15. Pada hari ke-16, tikus dikorbankan untuk diamati kualitas sperma dan pembuatan sediaan histologis testis (metode paraffin; pewarnaan HE). Seluruh data dianalisis menggunakan One-Way ANOVA dengan uji Duncan (P<0,05). Hasil penelitian menunjukan kualitas sperma dosis 0,4 g/Kg BB memiliki jumlah motilitas, jumlah sel, viabilitas dan morfologi sperma lebih tinggi dibandingkan antar perlakuan (P<0,05). Jumlah sel Leydig dan indeks spermatogenesis juga memperlihatkan dosis 0,4 dan 0,8 g/Kg BB lebih tinggi dibandingkan antar perlakuan (P<0,05). Kesimpulan menunjukan bahwa pemberian ekstrak etanol daun Bayam Merah dosis 0,4 g/Kg BB dapat berpotensi dalam melindungi kualitas sperma dan organ testis Tikus Wistar yang diinduksi parasetamol dosis toksik selama 15 hari.
Komposisi Spesies dan Kepadatan Nyamuk Dewasa di Daerah Perbukitan di Kabupaten Muara Enim, Sumatera Selatan
Authors: Dalilah Dalilah , Anwar Chairil , Handayani Dwi , Prasasty Gita Dwi , Syarifudin Din , Saleh Irsan , Warni Suldfa Esi
Published: November 2024

Nyamuk merupakan serangga dari ordo Diptera yang dapat menularkan penyakit parasit dan virus. Lima dari 18 genus nyamuk di Indonesia berperan sebagai vektor yakni Aedes, Culex, Anopheles, Mansonia dan Armigeres. Penelitian ini bertujuan untuk mengidentifikasi keragaman, menghitung kepadatan, serta paparan gigitan nyamuk di Desa Pulau Panggung, Kabupaten Muara Enim, Sumatera Selatan. Penangkapan nyamuk dilakukan pada bulan Juli 2023 dengan tiga metode: human landing collection, resting collection dan cattle bait collection. Kepadatan dan risiko paparan nyamuk dihitung berdasarkan Man Bitting Rate (MBR) dan Man Hour Density (MHD). Sebanyak 2131 nyamuk dari 16 spesies berhasil ditangkap (479 nyamuk dengan metode human landing dan resting collection; 1173 nyamuk dari cattle bait collection). Nyamuk Culex quinquefasciatus, Culex vishnui, dan Armigeres subalbatus memiliki nilai tertinggi pada perhitungan kepadatan nyamuk per spesies yang hinggap tiap jamnya berturut-turut 0,79/jam, 0,21/jam, dan 0,17/jam untuk UOD dan 1,33/jam, 2,29/jam dan 2,04/jam untuk UOL. Anopheles nigerrimus ditemukan pada UOL dengan nilai MHD 0,42/jam. Komposisi spesies nyamuk di Desa Pulau Panggung Kabupaten Muara Enim cukup beragam dengan ditemukannya lima genus dan 16 spesies nyamuk. Nyamuk Cx. quinquefasciatus, Cx. vishnui, Cx tritaeniorhynchus, dan Ar. subalbatus merupakan nyamuk yang mendominasi pada metode human landing colletion. Kepadatan keempat nyamuk tersebut tinggi dalam menggigit per jam dan lebih menyukai menggigit di luar rumah. Nyamuk Anopheles vagus merupakan nyamuk yang mendominasi pada metode cattle bait collection. Komposisi spesies dan kepadatan nyamuk di Desa Pulau Panggung cukup tinggi. Oleh karena itu, diperlukan pengendalian vektor untuk mencegah penyakit infeksi.
The Cultural and Muamalah Perspective on the Practices of Paroan Cooperation Agreements among Rice Farmers
Authors: Mongkito Abdul Wahid , Putra Trisno Wardy , Rahayu Siti , Hasanah Nurul
Published: November 2024

This research aims to explain how the practice of paroan cooperation is implemented among rice farmers in Cialam Jaya Village, Konda District, how it is adapted from the perspective of cultural and Islamic traditions, and how it is adapted to the muzara'ah contract. This type of qualitative research uses several techniques, namely observation and interviews. The data analysis method used is data reduction; the results of the research show that in Cialam Jaya Village, Konda District, many people carry out cooperation agreements or what they call Paroan cooperation agreements, which are almost similar to the Muzara'ah agreement in Islam to carry out profit sharing cooperation in rice farming, but this does not mean that the people of Cialam Jaya village, Konda District, carry out the Paroan cooperation agreement in a haphazard manner but rather with the principles of transactions contained in Islam. The majority of Muslim communities make Paroan and muzara'ah cooperation contracts commensurate with fulfilling three elements in implementing the muzara'ah contract: (1) the existence of a contract or agreement between the land owner and the cultivator, (2) there is a profit-sharing system with a specific ratio, (3) the land owner provides all needs (seeds, fertilizer).
When Hope Fades: Considering Self-Esteem, Depression, Social Support, Loneliness in The Context of Suicide Prevention
Authors: Çifci Fatmanur , Nikhat Ayesha , Shen Yuyang
Published: November 2024

Individuals engaging in suicidal ideation or making suicide attempts are a substantial demographic group to investigate preventive mental health due to inherent high risks associated with potential outcomes of suicidal behaviors for public health. Factors increasing the likelihood of suicide might include self-esteem, depression, social support, loneliness, or certain demographic profiles, and the purpose of this study is to synthesize these variables to advocate for effective strategies to intervene in cases of suicide. Specifically, this commentary aims to identify suicidal coping mechanisms and psychological resilience factors among suicidal individuals through the enhancement of self-esteem, intervention of depressive symptoms, and promotion of connectedness. Through discussing the determinants that might play a role in the onset of suicidal thoughts and suicide attempts, this paper has important implications for suicide prevention strategies and the identification of psychological resilience factors that can predict suicidal thoughts and suicide attempts in individuals.
On the need to report the variability and data used in the determination of xylem vulnerability curve parameters
Authors: Urli Morgane , Périé Catherine , Thiffault Nelson , Coyea Marie R. , Pepin Steeve , Lambert Marie-Claude , Munson Alison D.
Published: November 2024

Vulnerability curves to cavitation (VC) and their derived parameters, such as P50, are increasingly used and reported to assess forest vulnerability to drought and predict forest responses to climate change. Forest practitioners and policy-makers are encouraged to rely on these parameters to support species selection based on their sensitivity to drought. However, in the majority of studies, we consider that the variability of VC parameters is not clearly reported nor considered, which can lead to counterproductive decisions. In this opinion paper, we demonstrate the importance of precisely reporting the variability around VC parameters and the sources of this variability (plant materials, methods, etc.). We also identify the information that should be provided when reporting mean values of VC parameters. To support our argument, we built VCs for three Picea species and Pinus strobus, using different methods, and compared the value of P50 determined in our experiments with values from a literature review.
High and variable hydraulic resistance to embolism among three Asian juniper species
Authors: Larter Maximilian , Akhmedov Akbar , Payne Cayley , Delzon Sylvain , Klein Tamir
Published: November 2024

Over a background of a warming planet, with increasing occurrence of extreme climate events, junipers stand out as tree species thriving in inhospitable environments, enduring the cold, hot, and dry. Being part of the drought-adapted Cupressaceae Gray, some juniper species have been identified having among the most negative P50 values (vulnerability to xylem embolism) in the plant kingdom. Still, previous analyses skipped many Asian juniper species, some of which growing in arid conditions. Here we measured xylem vulnerability to embolism in three, yet unexplored, Asian juniper species: Juniperus drupacea growing in Israel, and J. pseudosabina and J. seravschanica growing in Uzbekistan. Branches were sampled from five trees per species and vulnerability curves were constructed using the Cavitron method, from which P50 was calculated. Xylem anatomy measurements were used to define torus overlap and maximum xylem specific hydraulic conductivity. These hydraulic traits were tested against species and site climate and in the context of species phylogeny. P50 of the Eastern Mediterranean J. drupacea was -11.8±0.3 MPa, much more negative than that of the two Asian junipers measured here, but within the same range of values measured in European and North American junipers. P50 was more negative with mean annual temperature and with aridity index of the driest quarter at both species (climatic conditions at the entire distribution range) and site levels. We did not find the relationships generally found in conifers between the properties of xylem pit membrane and vulnerability to embolism, nor with the overall aridity of the environment. To the best of our knowledge, our observations identify J. drupacea as the most drought-tolerant tree species in Asia.
La responsabilidad civil empresarial por el vertimiento de aguas residuales: a propósito de la aplicación del principio contaminador pagador
Authors:   , Fernandez Espino Jennifer Marilyn
Published: November 2024

La importancia indiscutible de la responsabilidad civil empresarial en relación con el vertimiento de aguas residuales y su conexión con el principio del contaminador pagador constituye un tema de gran relevancia. Por lo tanto, el propósito general de la investigación fue examinar cómo se aplica este principio en el ámbito empresarial y su relación con la responsabilidad civil. La investigación se llevó a cabo bajo un enfoque cualitativo y un diseño no experimental de tipo descriptivo. La población objeto de estudio fueron las empresas que realizan vertimientos de aguas residuales y los profesionales del derecho que participan en casos de responsabilidad civil. La muestra, conformada por expertos en la materia, fue seleccionada de manera no probabilística. Los resultados obtenidos indican que la aplicación del principio contaminador pagador en el ámbito de la responsabilidad civil empresarial es esencial para asegurar la preservación del medio ambiente y la salud pública. Asimismo, se concluye que las empresas deben adoptar medidas preventivas para evitar el vertimiento de aguas residuales y mitigar su impacto ambiental.
Impacto de la infección por el virus del dengue en pacientes con infecciones concurrentes en la población peruana
Authors:   , Gonzales Manchego Flavio Antonio
Published: November 2024

Introducción. El dengue es la enfermedad viral transmitida por mosquitos más importante a nivel mundial, especialmente en países en desarrollo de América Latina, constituyendo un problema de salud pública significativo. En Perú, la combinación del dengue con la pandemia de COVID-19 ha presentado un desafío considerable en términos de diagnóstico y tratamiento. Algunos estudios sugieren que la coinfección de estas dos enfermedades puede aumentar el número de pacientes que requieren cuidados intensivos y ventilación mecánica. Además, la concurrencia de tuberculosis y dengue también es posible, requiriendo más investigación para evaluar su impacto. La coexistencia de VIH y dengue ha sido documentada, al igual que la co-circulación de Zika y Chikungunya con dengue en zonas endémicas, lo que puede resultar en cuadros clínicos más graves. Objetivo: Analizar sistemáticamente el impacto de la infección por el virus del Dengue frente a otras infecciones concurrentes en la población peruana. Métodos: Se realizaron búsquedas de la literatura (hasta el 2 de mayo del 2024) utilizando como base de datos PubMed y Scopus. Adicionalmente, se realizaron búsquedas manuales en las siguientes bibliotecas virtuales: Scientific Electronic Library Online – SciELO Perú, la Biblioteca Virtual del Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica (CONCYTEC) y el Registro Nacional de Trabajos de Investigación (RENATI) con el propósito de reunir la mayor cantidad posible de artículos relacionado con el tema de investigación. Resultados: Se recuperaron un total de 374 artículos mediante la estrategia de búsqueda. Luego de eliminar duplicados y el proceso inicial de selección según criterios de inclusión se recopilaron 42 artículos para lectura de texto completo; de los cuales 12 se consideraron elegibles para esta revisión sistemática. Para la coinfección entre dengue y covid-19 se notificaron 566 casos reportados, de los cuales 3 se documentaron como casos de dengue grave y 15 de ellos fallecieron…
Use of Drone Technology for Methane Emissions Quantification - Level 4 and Level 5 Reconciliation as Per OGMP
Authors: Bouilhol P. , Almarzooqi H. , Ali Abdulla F. Moosa , Al Hammadi A. , Sasidharan S. , Bhagavathi P. Kumaresan , Almemari F. , Jaber T. , Vaswani Y. , Rasulova Z.
Published: November 2024

Methane emissions are the most potent Green House Gases from the Oil & Gas Industry. ADNOC Onshore is developing across its assets several technologies to monitor and quantify these emissions as per Oil & Gas Methane Partnership 2.0 (reference #1: United Nations Environment Programme). This paper is developing a case with the use of Drone technology -first application within ADNOC- and how it has been used to make the reconciliation between level 4 and level 5 monitoring. ADNOC Onshore pioneered in 2023 across all ADNOC Group Companies the use of Drone to monitor and quantify methane emissions from a Central Degassing Station (CDS). All the methane emissions sources were monitored by the Drone: Fugitives, Flares, Gas Fired Heaters, Oil storage and Produced Water tanks allowing to have a quantification and mapping of the methane emissions. The use of others ground technologies, High Flow Sampler (HFS) and Optical Gas Imaging Camera (OGI), in comparison with Drone's sensors has allowed us to identify some gaps between technologies and consider these gaps in our global methane quantification. A reconciliation between level 4 and level 5 measurement has been conducted as per Oil & Gas Methane Partnership 2.0 (OGMP 2.0) requirements. OGMP 2.0 is the new reporting framework aiming to improve reporting accuracy and transparency of CH4 emissions in the Oil&Gas sector. This reconciliation has been an opportunity to improve site measurement / calculations for emissions and increase the awareness to the operational teams. Reconciliation is the process of comparing source-level (Level 4) inventories with independent site-level measurements to produce Level 5 asset emissions estimates. Site-level measurements complement - rather than replace - source-level estimates, and the process of reconciliation helps improve accuracy, thoroughness and confidence in reported emissions. Reconciliation is an iterative process of investigation, year over year, and should not be thought of as a one-off comparison of two independent values. The process, like knowledge, will evolve over the years – the focus is on making credible progress year over year (reference #1: United Nations Environment Programme).

This paper is sharing the operational feedback on the use of Drone for methane measurement and as well the lessons learned following the reconciliation process with concrete recommendations. Some insights on observations made with the use of different technologies to quantify the methane emissions are developed.
Detecting Verbal Auditory Hallucination Among Schizophrenia Patients by Integrating Alternate Auditory Attention Tasks in Contralateral Suppression of Otoacoustic Emissions
Authors: Awang Che Muhammad Amir Che , Wahab Noor Alaudin Abdul , Maamor Nashrah , Tahir Siti Aisyah Mohammad , Zakaria Mohd. Normani , Wahab Suzaily
Published: November 2024

Subjective evaluations of verbal auditory hallucinations (VAH) in schizophrenia have limitations; thus, combining them with objective measures like neuroimaging may provide more accurate insights into brain activity during VAH episodes. However, neuroimaging is often costly and time-consuming, prompting the search for alternative methods. This study explores the integration of ALternate AUDItory AttentioN (ALAUDIN©) tasks with Contralateral Suppression of Otoacoustic Emissions (CSOAE) as a rapid and cost-effective approach to detect VAH in schizophrenia patients. A total of 57 healthy controls (HC) and 10 schizophrenia patients; five with active and five with passive VAH; participated. Various contralateral stimuli, including white noise (WN) alone and WN combined with auditory attention tasks, were used to evaluate CSOAE. While no significant differences in suppression were found between the left and right ears across all groups, patients with active VAH demonstrated significantly higher suppression than HC for specific stimuli (CS4). Notably, incorporating ALAUDIN© tasks did not significantly enhance suppression in HC or patients with passive VAH but descriptively increased suppression in those with active VAH. These results suggest that ALAUDIN©-CSOAE may effectively differentiate schizophrenia patients with VAH from healthy individuals, warranting further research with larger sample sizes to validate these findings.
Family-based genetics identifies association of CUBN, IL1RL1 and PRKN variants with leprosy in Bangladesh
Authors: Santos Andressa Mayra , Tió-Coma Maria , Garcia-Codina Gal·la , Verhard Els M. , Sartori Priscila Verchai Uaska , Roy Johan Chandra , Khatun Marufa , Alam Khorshed , Richardus Jan Hendrik , Mira Marcelo Távora , Geluk Annemieke
Published: November 2024

Previous studies have demonstrated the association between the occurrence of leprosy and several host genetic variants that can vary across different ethnic groups and populations. Since leprosy susceptibility genes have not been investigated in detail in the Bangladeshi population that still includes highly endemic areas, we here studied known associations between leprosy and 13 genetic markers located in 11 genes in a leprosy endemic area in Bangladesh for which an association with leprosy was previously found in an isolated former leprosy colony in the Brazilian Amazon. For this study, a family-based analysis using 60 parent-affected child trios was performed, followed by a case-control study of 210 leprosy patients and 189 healthy controls from the same area (endemic controls). Genotypes for 11 markers were determined by TaqMan SNP genotyping. In the family-based study, a significant association was found between leprosy and three single nucleotide polymorphisms, rs1801224, rs13001714, and rs1801582, located in the CUBN, IL1RL1, and PRKN genes, respectively. These findings were not replicated in the case-control sample. Variants in the CUBN, IL1RL1, and PRKN genes were associated with leprosy in Bangladesh, validating the initial Brazilian finding in a population sample of distinct ethnic background.
PD-L1 expression assessed by four FDA-approved PD-L1 immunohistochemical assays in clear cell renal cell carcinoma: concordance and association with prognosis
Authors: Koh Yoko , Kato Taigo , Yamamichi Gaku , Tomiyama Eisuke , Matsushita Makoto , Hatano Koji , Kawashima Atsunari , Nonomura Norio , Uemura Motohide
Published: November 2024

Therapeutic strategies for advanced renal cell carcinoma have advanced dramatically since the approval of immune checkpoint inhibitors. To date, four PD-L1 assays have been established as companion diagnostic tools for checkpoint inhibitors. However, all PD-L1 assays present several limitations in terms of concordance and compatibility. In this study, we constructed tissue microarrays from 286 clear cell renal cell carcinoma (ccRCC) tissue samples and evaluated PD-L1 expression using the 22C3, 28 − 8, SP142, and SP263 PD-L1 assays. Detection of PD-L1 expression in tumor cells was very low for all PD-L1 assays tested, although expression of PD-L1 in immune cells in the tumor area was 14.7% for 22C3, 16.1% for 28 − 8, 2.1% for SP142, and 15.0% for SP263. PD-L1 expression on immune cells assessed by 28 − 8 showed a moderate pairwise concordance with other assays (κ statistics: 0.52 with 22C3, 0.16 with SP142, and 0.46 with SP263). Patients with PD-L1 expression in immune cells evaluated using 22C3, 28 − 8, and SP263 showed significantly worse cancer-specific survival. PD-L1 expression on renal cell carcinoma tissues assessed using 22C3, 28 − 8, SP142, and SP263 did not show good concordance. In conclusion, differences between these assays should be considered when evaluating the results of immunohistochemical findings.
Oncogenic Function of Growth Arrest-specific Transcript 5 by Competing with miR-423-3p to Regulate SMARCA4 in Hepatocellular Carcinoma
Authors: Nam Suk Woo , Kim Sang Yean , Ha Jin Woong , Na Min Jeong , Jeon Soyoung
Published: November 2024

Long non-coding RNA growth arrest-specific transcript 5 (GAS5) has been identified as a tumor suppressor due to its downregulation in several cancers. However, our comprehensive analyses revealed aberrant overexpression of GAS5 in various cancers, with a direct association with SMARCA4 in hepatocellular carcinoma (HCC). Differential expression analyses were conducted using publicly available transcriptome datasets. Functional studies of GAS5 and its downstream targets in HCC were performed via siRNA-mediated knockdown in various HCC cell lines, in vivoxenograft mouse models, and spontaneous liver cancer models in Ras-transgenic mice. We discovered that METTL3-mediated N6-methyladenosine modification promoted IGF2BP2 binding, stabilizing GAS5 in HCC. GAS5 expression was significantly upregulated in a large cohort of solid cancer patients, including HCC. Targeted disruption of GAS5 resulted in notable inhibition of growth and proliferation in HCC cells. Further analyses demonstrated that GAS5 enhanced in vitro tumorigenesis and metastatic potential of HCC cells. MicroRNA target prediction and functional validation indicated that GAS5 shared a miR-423-3p binding element with SMARCA4 mRNA, functioning as a competing endogenous RNA. This interaction was validated in in vitro tumorigenesis assays and in vivo mouse models. Additionally, a synergistic effect was observed with a vehicle containing a siRNA mixture targeting both GAS5 and SMARCA4 in these animal models. m6A methylation-mediated IGF2BP2 binding stabilizes GAS5, which functions as a ceRNA for miR-423-3p, thereby enhancing the translation of SMARCA4 mRNA. GAS5 acts as a crucial regulator of the oncogenic SMARCA4 in hepatocellular carcinogenesis, presenting a potential therapeutic target for the treatment of liver malignancies.
Integrated analysis of single-cell and bulk transcriptomic data reveals altered cellular composition and predictive cell types in endometriosis
Authors: Chen Meihong , Shen Yan , Jiang Guanqun , Wang Liqun , Chen Qi
Published: November 2024

The early diagnosis of endometriosis is delayed and the clinical treatment is difficult, which causes severe economic burden to the patients. A comprehensive study of the cell classification and composition of endometriosis, is essential for early diagnosis and pathogenesis. This study utilized single-cell data as a reference to accurately deconvolute cell types within bulk transcriptomics data. Five main cell types in endometriosis (epithelial cells, mesenchymal cells, endothelial cells, lymphocytes, and myeloid cells) revealed varying degrees of change compared to healthy controls. Notably, epithelial cells significantly decreased while mesenchymal cells increased. However, MUC5B epithelial cells, showed an increasing trend, consistent with the increasing trend of dStromal-late mesenchymal cells and eM2 macrophages.Pathway enrichment analysis revealed the EMT signalling pathway dominated in endometriosis. Additionally, a random forest method based on cell types could successfully distinguish endometriosis patients from healthy control, which illustrates the potential value of cell types in early diagnosis.
Optimizing Spinal Infection Classification in Whole-Slide Images via Graph Convolutional Network and Model Uncertainty Integration
Authors: Liu Chaoyeu , Cheng Yongxiang , Wang Jin , Zhao Shuqing , Yuan Sen , Pang Chun guang , Li Ting , Hao Yanke , Zhang Qiang
Published: November 2024

Background
Spinal infections such as pyogenic spondylitis, spinal tuberculosis, and brucellar spondylitis are severe conditions that can lead to significant spinal damage and chronic pain. Whole-slide imaging (WSI) provides valuable visual information in pathological diagnoses. However, owing to the complexity and high dimensionality of WSI data, traditional manual diagnostic methods are often time-consuming and prone to errors. Therefore, developing an automated image analysis method is crucial to enhance the diagnostic accuracy and efficiency of WSI for spinal infections.
Methods
This study employed a novel framework that combines Graph Convolutional Networks (GCNs) with uncertainty quantification techniques to classify WSI images of spinal infections. A graph was constructed from segmented regions of the WSI, where nodes represented segmented pathological features and edges represented spatial relationships. The model was trained using a dataset of 422 cases from a provincial center for disease control and prevention and annotated for tuberculosis, brucellosis, and purulent spondylitis. The performance metrics were accuracy, precision, recall, and F1 scores.
Results
The integrated GCN model demonstrated a classification accuracy of 87%, recall of 85%, and F1 score of 0.86. Comparative analyses revealed that the GCN model exhibited a 10% higher performance than that of traditional CNN models. Moreover, the GCN model effectively quantified uncertainty and enhanced confidence in diagnostic decisions.
Conclusions
Integrating GCNs with model uncertainty enhances the accuracy and reliability of WSI image classification in pathology. This method significantly improves the capture of spatial relationships and identification of pathological features of spinal infections, offering a robust framework for supporting diagnostic and therapeutic decisions in medical practice.
The consequences of Fournier's gangrene in the penis or scrotum: the role of electrophotobiomodulation in conservative therapy
Authors: Elmelegy Nader
Published: November 2024

Background:
In 1883, Jean Alfred Fournier initially defined Fournier's gangrene as a rapidly developing and potentially lethal necrotizing fasciitis of the skin and soft tissue of the abdominal wall, scrotum, and perineal area. The optimal reconstruction technique is still up for debate.
Objective:
to assess how well E-light works for treating the aftereffects of post-fournier's gangrene.
Patients and Method:
E-light sessions were used to treat sixteen male patients with isolated penile or scrotal post-Fournier's gangrene raw areas.
Results:
Twelve patients (75%) had an excellent satisfaction rate, and four (25%) rated it as good. No fair or poor results were documented.
Conclusion:
Since E-light is an effective, easy, and affordable treatment with positive outcomes and minimal side effects, we advise using it to treat post-Fournier's gangrene isolated bare areas of the scrotum and penis.
The Causes of Oral Mucosal Lesions: A Retrospective Study Of Patients Attending Iranian Hospital
Authors: Eghbali Masoud , Alamdari Hamideh Azimi , Moghaddam Reza Forughi , Kargar Bita , Jesri Shabboo , Abdollahzadeh Ali , Hajialigol Amirhossein
Published: November 2024

Introduction: Oral ulcers are one of the most common reasons for patients to visit dentists and dermatologists, and their causes can vary from a simple trauma to a malignancy such as squamous cell carcinoma or a debilitating disease such as pemphigus. Geographical diversity in the field of demographic information of common oral ulcers indicates the need for a comprehensive study on this category of patients in the region, so that it may be possible to find new patterns in the diagnosis and treatment of oral ulcers by examining the recorded information.the present study aimed to investigating the causes of oral erosions in Tabriz city.
Method and Materials: The present descriptive cross-sectional study was designed to investigate the causes of oral erosions in the patients of Sina Hospital clinic from March 2023 to March 2024. After obtaining the code of ethics from the ethics committee of Tabriz Azad University Faculty of Medical Sciences, the files of 89 patients who underwent a biopsy of the lesion due to the presence of oral lesions, and their pathology results are available in the laboratory department of this center, were examined. In the following, the researcher examined the files of the target patients, extracted information, and finally, the collected information was analyzed based on the objectives of the study using SPSS version 27.0.0.0 software.
Results: The average age of the studied patients was 52.4 ± 18.6 years and 46 patients (52.2%) were Female. The buccal mucosa was the most common site of ulcer with a frequency of 68 people (76.4%). There was only one wound in 43 patients (48.9%), three wounds in 38 patients (43.2%), and two wounds in 6 patients (6.8%). In 5 patients (5.6%), they also mentioned the previous history of oral ulcers. Also, in most of the patients (77 patients, 87.5%), the presence of the ulcer lasted for more than 2 weeks. vulgaris with the frequency of 25 people (28.4%) was the most common pathology identified. After that, SCC with a frequency of 16 people (18.2%) and lichen planus with a frequency of 9 people (10.2%) were other common lesions in the studied patients.
Conclusion: According to the results obtained from the present study, men had more oral lesions than women. Also, in the oral environment, the buccal mucosa has been the most common site of ulceration. Pemphigus vulgaris is the most common cause of mouth ulcers among the identified pathologies.
How long do patellofemoral joint arthroplasties last?
Authors: Vella-Baldacchino Martinique , Chughtai Dean , Kow Jonathan , Carr Charlotte , Coyle Amelie , Farrow-Foster Amelia , Russell Jemima , Liddle Alexander
Published: November 2024

Patellofemoral arthroplasty is a offered to patients with isolated patellofemoral arthritis to restore function and reduce pain. This systematic review attempts to address long-term survivorship of the replacement, with conversion to a total knee arthroplasty (TKA) as an endpoint. Survival estimates from multiple case series and national registries were pooled to calculate survival at 5, 10, 15 and 20 years, respectively. As a secondary outcome, the type and number of complications were recorded. As arthroplasty registries do not report this information, this data was only included from publicly available series. 1015 eligible articles were identified, with 21 reporting survival and reasons for revision or complications. Data from registries were extracted. Using publicly available results from international joint registries, survival at 5 and 10 years were 90.30% (95% CI 88.32 to 92.27) and 82.23% (95% CI 78.90 to 85.56), respectively. However, long-term survivorship decreased to 73.74% (95% CI 69.12 to 78.37) and 72.68% (95% CI 69.58 to 75.53) at 15 and 20 years. Therefore, this highlights the importance of careful patient selection and the need for further research to improve long-term results. Future research should focus on reasons for pain in the postoperative period, including overall survival.
Analysis of plasma heparanase and heparin-binding protein levels in critically ill COVID-19 patients
Authors:   , Brkic Djurdjina
Published: November 2024

Background The demonstrated study examines plasma concentrations of heparanase (HPSE) and heparin-binding protein (HBP) in critically ill COVID-19 patients, admitted to the intensive care unit. Rooted in the hypothesis that HPSE and HBP present key indicators of endothelial injury, the study aims to evaluate their correlation with COVID-19 disease severity. Methods This is a prospective, single-center observational study, including 47 COVID-19 patients, treated in the intensive care unit. Daily assessed HPSE and HBP plasma levels were compared with those obtained in 10 healthy controls. Statistical analysis includes t-tests, Mann-Whitney U-tests and log-rank tests for survival analyses. Results The results depicted markedly elevated HPSE and HBP levels in COVID-19 patients compared with healthy controls. In survivors, overall increased HPSE plasma levels were observed, whereas nonsurvivors exhibited elevated HBP levels. An impact of age, arterial hypertension and diabetes mellitus on HPSE and HBP concentrations has not been determined. The study additionally demonstrated an effect of heparin and low-molecular-weight heparin on HPSE/HBP plasma levels. Conclusion This study varifies the role of HPSE and HBP as biomarkers of COVID-19 disease severity and provides insights into the pathophysiology of endothelial injury in critically ill COVID-19 patients. Subsequent research could further explore the therapeutic implementations in modulating these biomarkers, thus promoting development of noval treatment methods for critically ill COVID-19 patients.
Characteristics of stem sings of EpCAM-negative and EpCAM-positive tumor cells in primary tumor and 2D and 3D cultures in breast cancer
Authors: Alifanov V. V. , Tretyakova M. S. , Grigoryeva E. S. , Buldakov M. A. , Tashireva L. A. , Kolegova E. S. , Andryuhova E. S. , Zavyalova M. V. , Denisov E. V. , Cherdyntseva N. V. , Perelmuter V. M.
Published: November 2024

One of the predictors of adverse prognosis in breast cancer is the overexpression of the EpCAM protein. However, a signifcant part of tumor cells have low or no EpCAM expression. The molecular features and the ability to grow in 2D and 3D cultures, indirectly refecting metastatic potential of tumor cells without EpCAM expression, have not been sufficiently studied.

The aim of the study was to compare phenotypic variants of stem cells and EMT depending on the EpCAM expression in the primary tumor of breast cancer and 2D and 3D cultures.

Material and Methods. The study included 7 patients with invasive breast carcinoma of no special type. Luminal A subtype was found in 2/7 patients (29 %), and luminal B HER2-negative molecular subtype was found in 5/7 patients (71 %). The patients didn’t have neoadjuvant chemotherapy. Cells from the primary tumor were cultured in 2D and 3D. The primary tumor cells, 2D and 3D cultures were phenotyped using fow cytometry with antibodies targeting: CD45, EpCAM (CD326), CD44, CD24, N-cadherin (CD325), and CD133, CK7/8, EpCAM (CD326).

Results. No significant differences were found in the frequency of detection and the number of cells exhibiting stem cell features among EpCAM- and EpCAM+ tumor cells, 2D and 3D cultures. All phenotypic variants of co-expression of stemness markers CD44, CD24, CD133, and ALDH were present both in the primary tumor and 2D/3D cultures. Stem cells in mammospheres had features of both epithelial and hybrid EMT phenotypes. In primary tumors and 2D/3D cultures, the smallest proportion consisted of stem cells with the CD44+CD24- phenotype and cells co-expressing CD44+CD24- with other stemness markers, while the largest proportion comprised stem cells with ALDH+ and ALDH+CD133+ phenotypes. Marked intra- and inter-tumor heterogeneity in the phenotypic composition of primary tumors, 2D, and 3D cultures was noted.

Conclusion. The results indicate that EpCAM-negative and EpCAM-positive tumor cells of luminal molecular subtype of breast cancer are capable of exhibiting various phenotypic variants of stemness and EMT in the primary tumor and 2D and 3D cultures. In most cases, individual tumor cells show co-expression of several stemness markers. The phenotypic composition of primary tumors, 2D and 3D cultures is characterized by pronounced intra- and inter-tumor heterogeneity.
Collaboration Efforts of Special Education Teachers in Turkey
Authors: Dayı Eylem , İnce Mehmet , Ataman Safa , Aksoy Veysel
Published: November 2024

Introduction: The roles of teachers, among the most important stakeholders in schools, have begun to change with changes in the world and the field of education. Legal regulations and inclusive education practices require that teachers collaborate with various stakeholders beyond merely teaching. In this respect, the objective of the present research is to examine the status of collaboration between special education teachers and other stakeholders in detail.
Method: To this end, the research was designed as a mixed-method study. First, quantitative data were collected and analyzed, followed by qualitative data collection and analysis to understand the findings better. The study used an explanatory sequential design to determine teachers' collaboration levels, particularly in special education. A specially developed Collaboration Scale in Special Education, which underwent validity and reliability analyses, was used for the descriptive analysis of quantitative data. Qualitative data were collected through semi-structured interviews, observations, and researcher diaries and analyzed using content analysis.
Findings: The quantitative results of the study showed no significant differences in collaboration levels among teachers based on gender, age, or professional experience. Qualitative data revealed that teachers engaged in limited and generally basic collaboration with administrators, paraprofessionals, and external stakeholders. Quantitative and qualitative findings indicated that collaboration processes were generally superficial and more structured; therefore, focused processes were needed.
Discussion: The results of this study emphasize the necessity of structural arrangements and changes to establish effective collaboration between internal and external school stakeholders. Implementing the necessary regulations to ensure effective collaboration is considered highly important for the education of individuals with special needs.
To Remember and Forget: Reconstructing the Imaginary Reality of the Nationalism of Former East Timorese Refugees in Sukabitete, East Nusa Tenggara
Authors: Soehadha Moh
Published: November 2024

The release of the Province of East Timor from Indonesia in 1999 resulted in the displacement of East Timorese from the East Timor region to several provinces in Indonesia. Some refugees remained in Indonesia and chose to become Indonesian citizens. As new citizens, the former East Timor refugees experienced a struggle to foster a sense of nationalism toward Indonesia. This article originates from the results of qualitative reflective research using literature data and archival notes from former East Timor refugees, which were analyzed using ethnographic content analysis and rhetorical analysis methods. The study concludes that memories of past life in East Timor and the present reality of life in ex-refugee settlements in Indonesia generate diverse imaginations about Indonesia. To build a sense of nationality, the former East Timorese refugees must reconstruct their imaginations about Indonesia based on the past and present realities they have experienced, which are not all imaginary.
ARABIC LINGUISTICS IN THE SCIENTIFIC HERITAGE OF I. YU. KRACHKOVSKY
Authors:   Russian Academy of Sciences Library, Pumpian Galina Z.
Published: November 2024

The article examines the contribution of Academician I. Yu. Krachkovsky to Arabic linguistics. There are about 40 of his works in this field — linguistic publications themselves or articles of various subjects, in which there were comments made “along the way” on some issues of Arabic linguistics. There are four main directions in Krachkovsky’s linguistic works: 1) historical dialectology; 2) modern dialects and issues of the language situation in the Arab world; 3) arabic literary language — primarily issues of lexicology and lexicography, foreign borrowings, textual studies; 4) teaching and organizational, methodological and conceptual developments, including the initiative in the study of Central Asian Arabic dialects, Arabic-language documents in the Caucasus. All the linguistic material of I. Yu. Krachkovsky was collected and systematized, the most characteristic of his works in these areas were noted and analyzed with examples, their significance in science was indicated, including for the first time in such a section as historical Arabic dialectology. Although linguistics was not one of the priority areas in Krachkovsky’s work, his efforts in this field made a significant contribution to Arabic studies thanks to his talent and intuition of a subtle philologist, breadth of semitological horizons and excellent acquaintance with the state of world science, scrupulousness, and vast teaching experience.
ON ADDITIONAL CONSTRUCTIONS IN THE LANGUAGE OF ANCIENT TURKIC RUNIC MONUMENTS
Authors:   St. Petersburg State University, Kamalova Shahnaz N.
Published: November 2024

This article explores the ways of expressing additional constructions in the language of ancient Turkic runic monuments. The study of the language of ancient Turkic runic monuments revealed that complements can be divided into two main types: direct and indirect. A direct complement can be expressed by an accusative case suffix or in a form without a case marker. Based on the use of accusative case suffixes, direct complements can be further divided into definite and indefinite. In the language of ancient Turkic runic monuments, an indirect complement can be expressed through various means, such as case forms of nominal parts of speech, verbal-nominal forms, postpositions as part of noun phrases, pronouns with a nominal meaning, noun phrases (izafet), and nominal-adjective forms. The aim of this study was to identify the ways of expressing additional constructions in the language of ancient Turkic runic monuments. During the study, additional constructions were identified, and examples were provided to illustrate the methods of their expression.
Type 2 Diabetes Mellitus and Parkinson's Disease: An Emerging Link
Authors: Teli Shubham , Shalavadi Mallappa , VM Chandrashekhar , Anawal Lingaraj
Published: November 2024

Parkinson’s Disease (PD) is a common neurodegenerative disease, characterized by
motor deficit. Diabetes Mellitus (DM) is a metabolic condition characterized by high glucose
levels in the blood. The prevalence of chronic disorders like PD has significantly risen in recent
decades. Emerging research has increasingly linked PD with Type 2 Diabetes Mellitus (T2DM)
in recent years. Apart from the β-cells of the pancreas, insulin is secreted by the choroid plexus
in the human brain which is essential for neuroprotection which regulates neurogenesis, oxidative
stress, synaptic plasticity, neuronal survival, and neuro-inflammation. The abnormalities in
the insulin signaling pathway may be responsible for neurodegeneration via insulin dysregulation,
from the aggregation of α-synuclein, neuroinflammation, mitochondrial dysfunction, and
altered synaptic plasticity. DM or Insulin resistance is considered a risk factor for the development
of PD. Due to the lack of treatment options for PD, consistent research is going on to find
a potential drug as a treatment option for PD, and due to its molecular links, the antidiabetic
drugs are considered as potential candidates for PD. This review will discuss the potential cellular
mechanisms shared between T2DM and PD as well as the role of antidiabetics in this disease
and clinical manifestations such as its severity and prognosis.