bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Various Acid Solutions as an Aid in Removing the OrthoMTA-Based Root Canal Filling
Authors: Chhabra Naveen , Parolia Abhishek
Published: June 2023

The objectives of this study were to compare the effects of various acid solutions combined with ultrasonics as an aid to remove mineral trioxide aggregate (MTA)-based root canal filling and to assess their effect on the surface topography and microhardness of root canal dentin. Materials and Method: Fifty human permanent single rooted and single canaled freshly extracted teeth were decoronated and sectioned apically to prepare the middle third of root sections of 5 mm length. The canals were prepared in a step-back manner. OrthoMTA was packed throughout the prepared canals. These root sections were incubated for one week and subsequently randomly allocated to five groups (n = 10) according to the OrthoMTA removal method: No treatment (NT); 5% glycolic acid + ultrasonics (5% GA+U); 10% glycolic acid + ultrasonics (10% GA+U); 10% citric acid + ultrasonics (10% CA+U); Distilled water + ultrasonics (DW+U). A 1 mm deep well was created within the coronal end of the set OrthoMTA. Wells were filled with each respective test solution and left for 5 min. Thereafter, further removal of OrthoMTA used a specific ultrasonic tip. Finally, the canals were flushed using 1 mL of the respective test solutions and activated with a Controlled Memory ultrasonic tip for two cycles of 20 s each followed by flushing with 1 mL of distilled water and paper point drying of the canals. Then, specimens were longitudinally split into two halves and examined under a scanning electron microscope (1000×) to assess the residual OrthoMTA and surface topography of root canal dentin. The Vickers surface microhardness of treated radicular dentin was measured using the HMV-2 microhardness tester. Result: Data were analysed using one-way ANOVA followed by Tukey’s post hoc test. Significant differences for residual OrthoMTA were observed between (10% GA+U) with (5% GA+U), (10% CA+U), (DW+U) and (NT) (p value < 0.01). In the context of microhardness, (5% GA+U) and (10% GA+U) showed statistically significant difference compared to (NT), (10% CA+U) and (DW+U) (p value < 0.01). Conclusion: 10% GA+U was superior to other tested groups in removing OrthoMTA, but it substantially reduced dentin microhardness.
One-Step Microwave-Assisted Hydrothermal Preparation of Zn-ZnO(Nw)-rGO Electrodes for Supercapacitor Applications
Authors: Bandas Cornelia , Nicolaescu Mircea , Popescu Mina Ionela , Orha Corina , Căprărescu Simona , Lazau Carmen
Published: June 2023

Zn-ZnO(Nw)-rGO hybrid electrodes for supercapacitor applications were successfully prepared in situ by a one-step microwave-assisted hydrothermal method by deposition of reduced graphene oxide (rGO) on the structure of ZnO nanowires grown on the Zn foil. During the hydrothermal treatment, two processes occur the reduction of graphene oxide (GO) and the deposition of rGO on the Zn-ZnO(Nw) support. The growth of ZnO nanowires was achieved by thermal oxidation below the melting point of the Zn foil in a controlled atmosphere. The as-obtained electrodes were assessed for structural, optical, and morphological properties by X-ray diffraction, Raman spectroscopy, ultraviolet-visible spectroscopy, SEM microscopy, and EDX analysis. The supercapacitor properties of the Zn-ZnO(Nw)-rGO hybrid electrodes were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge-discharge analysis. The CV curve reveals that the Zn-ZnO(Nw)-rGO hybrid structures work as negative electrodes and exhibit a non-ideal rectangle-like shape, suggesting that the as-synthesized structure behaves as a pseudo-capacitor. A maximum capacitance was determined to be 395.79 mF cm−2 at a scan rate of 5 mV s−1. Based on GCD analysis, the maximum specific capacitance of 145.59 mF cm−2 was achieved at a low power density of 2 mA cm−2. The cycle life assessment of the Zn-ZnO(Nw)-rGO hybrid electrode over a 250-cycle number was performed by CV and GCD analysis. The maximum retention rate of 120.86% was achieved from GCD analysis over 250 cycles for the Zn-ZnO(Nw)-rGO hybrid electrode.
Multiple-Criteria Heuristic Rating Estimation
Authors: Kędzior Anna , Kułakowski Konrad
Published: June 2023

One of the most widespread multi-criteria decision-making methods is the Analytic Hierarchy Process (AHP). AHP successfully combines the pairwise comparisons method and the hierarchical approach. It allows the decision-maker to set priorities for all ranked alternatives. But what if, for some of them, their ranking value is known (e.g., it can be determined differently)? The Heuristic Rating Estimation (HRE) method proposed in 2014 tried to bring the answer to this question. However, the considerations were limited to a model only considering a few criteria. This work analyzes how HRE can be used as part of the AHP hierarchical framework. The theoretical considerations are accompanied by illustrative examples showing HRE as a multiple-criteria decision-making method.
A Cost Optimisation Model for Maintenance Planning in Offshore Wind Farms with Wind Speed Dependent Failure Rates
Authors: Li Xiaodong , Song Xiang , Ouelhadj Djamila
Published: June 2023

This paper presents an optimisation model for cost optimisation of maintenance at an offshore wind farm (OWF). The model is created for OWF project developers to optimise strategic resources to meet their maintenance demand. The model takes into account various maintenance categories on a full range of wind turbine components; the failure rate associated with each component is dependent on wind speed in order to consider weather uncertainty. Weibull distribution is used to predict the probability of wind speed occurring during a given period based on available historical data. The performance of the proposed optimisation model has been validated using reference cases and a UK OWF in operation. Various optimal solutions are investigated for the problems with increased and decreased mean turbine failure rates as a sensitivity test of the model.
The Impact of Higher-Order Interactions on the Synchronization of Hindmarsh–Rose Neuron Maps under Different Coupling Functions
Authors: Mehrabbeik Mahtab , Ahmadi Atefeh , Bakouie Fatemeh , Jafari Amir Homayoun , Jafari Sajad , Ghosh Dibakar
Published: June 2023

In network analysis, links depict the connections between each pair of network nodes. However, such pairwise connections fail to consider the interactions among more agents, which may be indirectly connected. Such non-pairwise or higher-order connections can be signified by involving simplicial complexes. The higher-order connections become even more noteworthy when it comes to neuronal network synchronization, an emerging phenomenon responsible for the many biological processes in real-world phenomena. However, involving higher-order interactions may considerably increase the computational costs. To confound this issue, map-based models are more suitable since they are faster, simpler, more flexible, and computationally more optimal. Therefore, this paper addresses the impact of pairwise and non-pairwise neuronal interactions on the synchronization state of 10 coupled memristive Hindmarsh–Rose neuron maps. To this aim, electrical, inner linking, and chemical synaptic functions are considered as two- and three-body interactions in three homogeneous and two heterogeneous cases. The results show that through chemical pairwise and non-pairwise synapses, the neurons achieve synchrony with the weakest coupling strengths.
Non-Emptiness, Relative Coincidences and Axiomatic Results for the Precore
Authors: Hwang Yan-An , Liao Yu-Hsien
Published: June 2023

Taking into account the definition of the precore, we initially analyze its non-emptiness using duality results from linear programming theory. We then introduce the dominance core to investigate the coincident relations between the precore and dominance core. Finally, we propose specific reduction approaches to axiomatize the precore.
High-Conductive CsH2PO4 Membranes with PVDF-Based Polymers Additives
Authors: Bagryantseva Irina , Ponomareva Valentina , Kungurtsev Yuri
Published: June 2023

The study is devoted to one of the important problems of hydrogen energy—the comparative analysis and creation of novel highly conductive and durable medium-temperature proton membranes based on cesium dihydrogen phosphate and fluoropolymers. The proton conductivity, structural characteristics and mechanical properties of (1 − x)CsH2PO4-x fluoropolymer electrolytes (x-mass fraction, x = 0–0.3) have been investigated and analyzed. UPTFE and PVDF-based polymers (F2M, F42, and SKF26) with high thermal stability and mechanical properties have been chosen as polymer additives. The used fluoropolymers are shown to be chemical inert matrices for CsH2PO4. According to the XRD data, a monoclinic CsH2PO4 (P21/m) phase was retained in all of the polymer electrolytes studied. Highly conductive and mechanically strong composite membranes with thicknesses of ~50–100 μm were obtained for the soluble fluoropolymers (F2M, F42, and SKF26). The size and shape of CsH2PO4 particles and their distribution have been shown to significantly affect proton conductivity and the mechanical properties of the membranes. The thin-film polymer systems with uniform distributions of salt particles (up to ~300 nm) were produced via the use of different methods. The best results were achieved via the pretreatment of the suspension in a bead mill. The ability of the membranes to resist plastic deformation increases with the growth of the polymer content in comparison with the pure CsH2PO4, and the values of the mechanical strength characteristics are comparable to the best low-temperature polymer membranes. The proton-conducting membranes (1 − x)CsH2PO4-x fluoropolymer with the optimal combination of the conductivity and mechanical and hydrophobic properties are promising for use in solid acid fuel cells and other medium-temperature electrochemical devices.
Mother Leads with Her Heart: A Case Study of Women Worker Leaders in the Men-Dominated Forestry Sector
Authors: Larasatie Pipiet , Ulifah Chorirotun Nur
Published: June 2023

The masculine image and the notions of manual work and physical strength have resulted in more men workers in forestry. This gender imbalance has influenced the sector’s leadership environment, a critical element in the workplace setting for meeting organizational goals. This study aims to investigate women’s leadership dynamics in Perhutani, the only state-owned enterprise responsible for managing forest resources in Indonesia. Utilizing in-depth interviews with 11 women leaders from middle to top management levels, we found that there is a perceived physical limitation barrier to being a woman leader in Perhutani. To climb the career ladder, a support system from family, colleagues, and supervisors is important. However, family can be two sides of a coin, as it is also acknowledged as an internal constraint to being a woman leader. Our respondents acknowledge leadership labyrinth theory in being able to reach top leadership positions and tokenism theory, pointing out that the benefits of women leadership for Perhutani include the realization of gender equality, referring to the key performance index from the Government of Indonesia. For our respondents, being in a men-dominated sector is considered one of the attractive aspects of being a woman leader. They felt constantly challenged, but in a positive way. With these circumstances, our respondents showcase leadership styles in line with the theories of heart leadership and mother leadership. Heart-led leaders draw on the qualities of empathy, love, and vulnerability, while mother leadership means not only leading with the heart of a mother, but also with the mission, vision, and actions of a mother.
Right Ventricular Subclinical Dysfunction in SLE Patients Correlates with Metabolomic Fingerprint and Organ Damage
Authors: Deidda Martino , Noto Antonio , Firinu Davide , Piras Cristina , Cordeddu William , Depau Claudia , Costanzo Giulia , Del Giacco Stefano , Atzori Luigi , Mercuro Giuseppe , Cadeddu Dessalvi Christian
Published: June 2023

Systemic lupus erythematosus (SLE) is a chronic inflammatory disease, and several studies have suggested possible early RV involvement. Aim of the study was to evaluate the 3D echo parameters of the right ventricle (RV) and the metabolomic profile to correlate both with SLE severity. Forty SLE patients, free of cardiovascular disease, were enrolled and the following 3D parameters were evaluated: the RV ejection fraction (RV-EF), longitudinal strain of the interventricular septum (Septal LS), longitudinal strain of the free wall (Free-LS) and the fractional area change (FAC). In addition, a metabolomic analysis was performed. Direct correlations were observed between TAPSE values and the RV 3D parameters. Then, when splitting the population according to the SDI value, it was found that patients with higher cumulative damage (≥3) had significantly lower FAC, RV-EF, Septal LS, and Free-LS values; the latter three parameters showed a significant correlation with the metabolic profile of the patients. Furthermore, the division based on SDI values identified different metabolic profiles related to the degree of RV dysfunction. The RV dysfunction induced by the chronic inflammatory state present in SLE can be identified early by 3D echocardiography. Its severity seems to be related to systemic organ damage and the results associated with a specific metabolic fingerprint constituted by 2,4-dihydroxybutyric acid, 3,4-dihydroxybutyric acid, citric acid, glucose, glutamine, glycine, linoleic acid, oleic acid, phosphate, urea, and valine.
Measurement and Correction of Pointing Error Caused by Radio Telescope Alidade Deformation based on Biaxial Inclination Sensor
Authors: Xu Qian , Xue Fei , Wang Hui , Yi Letian
Published: June 2023

One of the key reasons for the deterioration of antenna pointing accuracy for radio telescopes is the deformation and tilt of antenna alidades, which primarily result from track unevenness and thermal gradients. A high-precision inclinometer measurement system is installed to investigate the tilt of the antenna alidade and the pointing errors caused thermally. An environment control box with a leveling base was designed to reduce the interference of the external environment, which proved to be effective in guaranteeing the zero-point stability and repeat accuracy of the inclinometer. The tilt of the alidade caused by the track unevenness was measured by a test of slowly rotating the antenna along the azimuth at windless nighttime. A 5-day antenna stationary test and a 48 h astronomical pointing error measurement were performed, which proved the inclinometer measurement system is capable of measuring the thermally induced inclinations with acceptable accuracy. Through a preliminary compensation experiment, the pointing error is compensated from 37″ to 12″, which shows that the application of the system has a good effect on improving the pointing accuracy of the antenna. The system with high measurement accuracy, good system stability, and low computational complexity, proves an effective tool for the radio telescope to solve the problem of real-time measurement and compensation for antenna pointing errors.
Tomato Plant Microbiota under Conventional and Organic Fertilization Regimes in a Soilless Culture System
Authors: Resendiz-Nava Carolina N. , Alonso-Onofre Fernando , Silva-Rojas Hilda V. , Rebollar-Alviter Angel , Rivera-Pastrana Dulce M. , Stasiewicz Matthew J. , Nava Gerardo M. , Mercado-Silva Edmundo M.
Published: June 2023

Tomato is the main vegetable cultivated under soilless culture systems (SCSs); production of organic tomato under SCSs has increased due to consumer demands for healthier and environmentally friendly vegetables. However, organic tomato production under SCSs has been associated with low crop performance and fruit quality defects. These agricultural deficiencies could be linked to alterations in tomato plant microbiota; nonetheless, this issue has not been sufficiently addressed. Thus, the main goal of the present study was to characterize the rhizosphere and phyllosphere of tomato plants cultivated under conventional and organic SCSs. To accomplish this goal, tomato plants grown in commercial greenhouses under conventional or organic SCSs were tested at 8, 26, and 44 weeks after seedling transplantation. Substrate (n = 24), root (n = 24), and fruit (n = 24) composite samples were subjected to DNA extraction and high-throughput 16S rRNA gene sequencing. The present study revealed that the tomato core microbiota was predominantly constituted by Proteobacteria, Actinobacteria, and Firmicutes. Remarkably, six bacterial families, Bacillaceae, Microbacteriaceae, Nocardioidaceae, Pseudomonadaceae, Rhodobacteraceae, and Sphingomonadaceae, were shared among all substrate, rhizosphere, and fruit samples. Importantly, it was shown that plants under organic SCSs undergo a dysbiosis characterized by significant changes in the relative abundance of Bradyrhizobiaceae, Caulobacteraceae, Chitinophagaceae, Enterobacteriaceae, Erythrobacteraceae, Flavobacteriaceae, Nocardioidaceae, Rhodobacteraceae, and Streptomycetaceae. These results suggest that microbial alterations in substrates, roots, and fruits could be potential factors in contributing to the crop performance and fruit quality deficiencies observed in organic SCSs.
Bacteremia in Severe Mastitis of Dairy Cows
Authors: Krebs Isabel , Zhang Yanchao , Wente Nicole , Leimbach Stefanie , Krömker Volker
Published: June 2023

The aim of this cross-sectional study was to investigate the occurrence of bacteremia in severe mastitis cases of dairy cows. Milk and corresponding blood samples of 77 cases of severe mastitis were bacteriologically examined. All samples (milk and blood) were incubated aerobically and anaerobically to also investigate the role of obligate anaerobic microorganisms in addition to aerobic microorganisms in severe mastitis. Bacteremia occurred if identical bacterial strains were isolated from milk and blood samples of the same case. In addition, pathogen shedding was examined, and the data of animals and weather were collected to determine associated factors for the occurrence of bacteremia in severe mastitis. If Gram-negative bacteria were detected in milk samples, a Limulus test (detection of endotoxins) was also performed for corresponding blood samples without the growth of Gram-negative bacteria. In 74 cases (96.1%), microbial growth was detected in aerobically incubated milk samples. The most-frequently isolated bacteria in milk samples were Escherichia (E.) coli (48.9%), Streptococcus (S.) spp. (18.1%), and Klebsiella (K.) spp. (16%). Obligatory anaerobic microorganisms were not isolated. In 72 cases (93.5%) of the aerobically examined blood samples, microbial growth was detected. The most-frequently isolated pathogens in blood samples were non-aureus Staphylococci (NaS) (40.6%) and Bacillus spp. (12.3%). The Limulus test was positive for 60.5% of cases, which means a detection of endotoxins in most blood samples without the growth of Gram-negative bacteria. Bacteremia was confirmed in 12 cases (15.5%) for K. pneumoniae (5/12), E. coli (4/12), S. dysgalactiae (2/12), and S. uberis (1/12). The mortality rate (deceased or culled) was 66.6% for cases with bacteremia and 34.1% for cases without bacteremia. High pathogen shedding and high humidity were associated with the occurrence of bacteremia in severe mastitis.
Implications of Pharmacokinetic Potentials of Pioglitazone Enantiomers in Rat Plasma Mediated through Glucose Uptake Assay
Authors: Spandana Tatineni , Goli Veera Venkata Nishanth , Rahamathulla Mohamed , Talath Sirajunisa , Osmani Riyaz Ali M. , Ahmed Mohammed Muqtader , Farhana Syeda Ayesha , Hussain Shalam Mohamed , Gurupadayya Bannimath
Published: June 2023

Pioglitazone, a PPAR-gamma activator used to diagnose hyperglycemia, was studied for its stereoselective deposition and active enantiomers in female albino Wistar rats. In accordance with USFDA recommendations, a bioanalytical technique was employed to assess the segregation of pioglitazone enantiomers in rat plasma with glimepiride as an internal standard. A Phenomenox i-Amylose-3 column (150 mm × 4.6 mm) of 5 µm was used for high-performance liquid chromatography (HPLC) with a mobile phase of 10 mM ammonium acetate buffer in Millipore water and acetonitrile in 60:40 (v/v) admixture with column temperature 35 °C, wavelength 265 nm, and flow rate 0.6 mL/min, respectively. Pioglitazone-S, Pioglitazone-R, and the internal standard had retention times of 3.1, 7.4, and 1.7 min, respectively. The study found that within-run and between-run precision ranged from 0.1606–0.9889% for Pioglitazone-R and from 0.2080–0.7919% for Pioglitazone-S, while the accuracy ranged from 99.86 to 100.36% for Pioglitazone-R and 99.84 to 99.94% for Pioglitazone-S. In addition, a non-radioactive glucose uptake assay was employed to examine the enantiomers in 3T3-L1 cell lines by flow cytometry. Significant differences were demonstrated in Cmax, AUClast (h*μg/mL), AUCINF obs (h*μg/mL), and AUC%Extrap obs (%) of Pioglitazone-R and S in female albino Wistar rats, suggesting enantioselectivity of pioglitazone.
Connexin 43 Phosphorylation: Implications in Multiple Diseases
Authors: Zhang Meng , Wang Zhen-Zhen , Chen Nai-Hong
Published: June 2023

Connexin 43 (Cx43) is most widely distributed in mammals, especially in the cardiovascular and nervous systems. Its phosphorylation state has been found to be regulated by the action of more than ten kinases and phosphatases, including mitogen-activated protein kinase/extracellular signaling and regulating kinase signaling. In addition, the phosphorylation status of different phosphorylation sites affects its own synthesis and assembly and the function of the gap junctions (GJs) to varying degrees. The phosphorylation of Cx43 can affect the permeability, electrical conductivity, and gating properties of GJs, thereby having various effects on intercellular communication and affecting physiological or pathological processes in vitro and in vivo. Therefore, clarifying the relationship between Cx43 phosphorylation and specific disease processes will help us better understand the disease. Based on the above clinical and preclinical findings, we present in this review the functional significance of Cx43 phosphorylation in multiple diseases and discuss the potential of Cx43 as a drug target in Cx43-related disease pathophysiology, with an emphasis on the importance of connexin 43 as an emerging therapeutic target in cardiac and neuroprotection.
Phenylketonuria (PKU) Urinary Metabolomic Phenotype Is Defined by Genotype and Metabolite Imbalance: Results in 51 Early Treated Patients Using Ex Vivo 1H-NMR Analysis
Authors: Cannet Claire , Bayat Allan , Frauendienst-Egger Georg , Freisinger Peter , Spraul Manfred , Himmelreich Nastassja , Kockaya Musa , Ahring Kirsten , Godejohann Markus , MacDonald Anita , Trefz Friedrich
Published: June 2023

Phenylketonuria (PKU) is a rare metabolic disorder caused by mutations in the phenylalanine hydroxylase gene. Depending on the severity of the genetic mutation, medical treatment, and patient dietary management, elevated phenylalanine (Phe) may occur in blood and brain tissues. Research has recently shown that high Phe not only impacts the central nervous system, but also other organ systems (e.g., heart and microbiome). This study used ex vivo proton nuclear magnetic resonance (1H-NMR) analysis of urine samples from PKU patients (mean 14.9 ± 9.2 years, n = 51) to identify the impact of elevated blood Phe and PKU treatment on metabolic profiles. Our results found that 24 out of 98 urinary metabolites showed a significant difference (p < 0.05) for PKU patients compared to age-matched healthy controls (n = 51) based on an analysis of urinary metabolome. These altered urinary metabolites were related to Phe metabolism, dysbiosis, creatine synthesis or intake, the tricarboxylic acid (TCA) cycle, end products of nicotinamide-adenine dinucleotide degradation, and metabolites associated with a low Phe diet. There was an excellent correlation between the metabolome and genotype of PKU patients and healthy controls of 96.7% in a confusion matrix model. Metabolomic investigations may contribute to a better understanding of PKU pathophysiology.
A Novel Dye-Modified Metal–Organic Framework as a Bifunctional Fluorescent Probe for Visual Sensing for Styrene and Temperature
Authors: Yang Jie , Ren Chaojun , Liu Min , Li Wenwei , Gao Daojiang , Li Hongda , Ning Zhanglei
Published: June 2023

A novel fluorescent probe (C460@Tb-MOFs) was designed and synthesized by encapsulating the fluorescent dye 7-diethylamino-4-methyl coumarin (C460) into a terbium-based metal–organic framework using a simple ultrasonic impregnation method. It is impressive that this dye-modified metal–organic framework can specifically detect styrene and temperature upon luminescence quenching. The sensing platform of this material exhibits great selectivity, fast response, and good cyclability toward styrene detection. It is worth mentioning that the sensing process undergoes a distinct color change from blue to colorless, providing conditions for the accurate visual detection of styrene liquid and gas. The significant fluorescence quenching mechanism of styrene toward C460@Tb-MOFs is explored in detail. Moreover, the dye-modified metal–organic framework can also achieve temperature sensing from 298 to 498 K with high relative sensitivity at 498 K. The preparation of functionalized MOF composites with fluorescent dyes provides an effective strategy for the construction of sensors for multifunctional applications.
Widely Targeted Metabolomics Reveals the Effects of Soil on the Metabolites in Dioscorea opposita Thunb.
Authors: Yang Lanping , Zhai Yangyang , Zhang Zhenzhen , Liu Zhenzhen , Hou Baohua , Zhang Baobao , Wang Zhenhui
Published: June 2023

Chinese yam (Dioscorea opposita Thunb. cv. Tiegun), a type of homologous medicinal plant, mainly grows in sandy soil (SCY) and loessial soil (LCY). However, the effects of the soil on the metabolites in SCY and LCY remain unclear. Herein, this study aims to comprehensively elucidate the metabolites in SCY and LCY. A UPLC-MS/MS-based, widely targeted metabolomics approach was adapted to compare the chemical composition of SCY and LCY. A total of 988 metabolites were detected, including 443 primary metabolites, 510 secondary metabolites, and 35 other compounds. Notably, 177 differential metabolites (classified into 12 categories) were identified between SCY and LCY; among them, 85.9% (152 differential metabolites) were upregulated in LCY. LCY significantly increased the contents of primary metabolites such as 38 lipids and 6 nucleotides and derivatives, as well as some secondary metabolites such as 36 flavonoids, 28 phenolic acids, 13 alkaloids, and 6 tannins. The results indicate that loessial soil can improve the nutritional and medicinal value of D. opposita.
Phytochemical Composition and Antioxidant Activity of Various Extracts of Fibre Hemp (Cannabis sativa L.) Cultivated in Lithuania
Authors: Judžentienė Asta , Garjonytė Rasa , Būdienė Jurga
Published: June 2023

The phytochemistry of fibre hemp (Cannabis sativa L., cv. Futura 75 and Felina 32) cultivated in Lithuania was investigated. The soil characteristics (conductivity, pH and major elements) of the cultivation field were determined. The chemical composition of hemp extracts and essential oils (EOs) from different plant parts was determined by the HPLC/DAD/TOF and GC/MS techniques. Among the major constituents, β-caryophyllene (≤46.64%) and its oxide (≤14.53%), α-pinene (≤20.25%) or α-humulene (≤11.48) were determined in EOs. Cannabidiol (CBD) was a predominant compound (≤64.56%) among the volatile constituents of the methanolic extracts of hemp leaves and inflorescences. Appreciable quantities of 2-monolinolein (11.31%), methyl eicosatetraenoate (9.70%) and γ-sitosterol (8.99%) were detected in hemp seed extracts. The octadecenyl ester of hexadecenoic acid (≤31.27%), friedelan-3-one (≤21.49%), dihydrobenzofuran (≤17.07%) and γ-sitosterol (14.03%) were major constituents of the methanolic extracts of hemp roots, collected during various growth stages. The CBD quantity was the highest in hemp flower extracts in pentane (32.73%). The amounts of cannabidiolic acid (CBDA) were up to 24.21% in hemp leaf extracts. The total content of tetrahydrocannabinol (THC) isomers was the highest in hemp flower pentane extracts (≤22.43%). The total phenolic content (TPC) varied from 187.9 to 924.7 (average means, mg/L of gallic acid equivalent (GAE)) in aqueous unshelled hemp seed and flower extracts, respectively. The TPC was determined to be up to 321.0 (mg/L GAE) in root extracts. The antioxidant activity (AA) of hemp extracts and Eos was tested by the spectrophotometric DPPH● scavenging activity method. The highest AA was recorded for hemp leaf EOs (from 15.034 to 35.036 mmol/L, TROLOX equivalent). In the case of roots, the highest AA (1.556 mmol/L, TROLOX) was found in the extracts of roots collected at the seed maturation stage. The electrochemical (cyclic and square wave voltammetry) assays correlated with the TPC. The hydrogen-peroxide-scavenging activity of extracts was independent of the TPC.
Investigating the Effects of Donors and Alkyne Spacer on the Properties of Donor-Acceptor-Donor Xanthene-Based Dyes
Authors: Rajapaksha Ishanka N. , Wang Jing , Leszczynski Jerzy , Scott Colleen N.
Published: June 2023

NIR dyes have become popular for many applications, including biosensing and imaging. For this reason, the molecular switch mechanism of the xanthene dyes makes them useful for in vivo detection and imaging of bioanalytes. Our group has been designing NIR xanthene-based dyes by the donor-acceptor-donor approach; however, the equilibrium between their opened and closed forms varies depending on the donors and spacer. We synthesized donor-acceptor-donor NIR xanthene-based dyes with an alkyne spacer via the Sonogashira coupling reaction to investigate the effects of the alkyne spacer and the donors on the maximum absorption wavelength and the molecular switching (ring opening) process of the dyes. We evaluated the strength and nature of the donors and the presence and absence of the alkyne spacer on the properties of the dyes. It was shown that the alkyne spacer extended the conjugation of the dyes, leading to absorption wavelengths of longer values compared with the dyes without the alkyne group. In addition, strong charge transfer donors shifted the absorption wavelength towards the NIR region, while donors with strong π-donation resulted in xanthene dyes with a smaller equilibrium constant. DFT/TDDFT calculations corroborated the experimental data in most of the cases. Dye 2 containing the N,N-dimethylaniline group gave contrary results and is being further investigated.
Tracking the Progression of the Simulated Bronze Disease—A Laboratory X-ray Microtomography Study
Authors: Wang Zedong , Xi Xiaoqi , Li Lei , Zhang Zhicun , Han Yu , Wang Xinguang , Sun Zhaoying , Zhao Hongfeng , Yuan Ning , Li Huimin , Yan Bin , Chen Jiachang
Published: June 2023

The internal three-dimensional characteristics of X-ray microtomography (micro-CT) has great application potential in the field of bronze corrosion. This work presents a method of simulating bronze disease based on an in situ micro-CT image to study the characteristics of the oxidative hydrolysis reactions of copper(I) chloride and copper(II) chloride dihydrate. A series of high-resolution reconstruction images were obtained by carrying out micro-CT at three key points throughout the experiment. We found that the reactions of copper(I) chloride and copper(II) chloride dihydrate showed different characteristics at different stages of the simulation in the micro-CT view. The method proposed in this work specifically simulated one single type of bronze corrosion and characterized the evolution characteristics of simulated bronze disease. It provides a new perspective to investigate bronze disease and can help improve the subsequent use of micro-CT to distinguish real bronze corrosions.
Impact of Intensive Glycemic Treatment on Diabetes Complications—A Systematic Review
Authors: Poonoosamy Juliana , Lopes Philippe , Huret Priscille , Dardari Randa , Penfornis Alfred , Thomas Claire , Dardari Dured
Published: June 2023

Diabetes complications can be related to the long duration of the disease or chronic hyperglycemia. The follow-up of diabetic patients is based on the control of chronic hyperglycemia, although this correction, if obtained rapidly in people living with severe chronic hyperglycemia, can paradoxically interfere with the disease or even induce complications. We reviewed the literature describing the impact of the rapid and intense treatment of hyperglycemia on diabetic complications. The literature review showed that worsening complications occurred significantly in diabetic microangiopathy with the onset of specific neuropathy induced by the correction of diabetes. The results for macroangiopathy were somewhat mixed with the intensive and rapid correction of chronic hyperglycemia having a neutral impact on stroke and myocardial infarction but a significant increase in cardiovascular mortality. The management of diabetes has now entered a new era with new therapeutic molecules, such as gliflozin for patients living with type 2 diabetes, or hybrid insulin delivery systems for patients with insulin-treated diabetes. Our manuscript provides evidence in support of these personalized and progressive algorithms for the control of chronic hyperglycemia.
Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
Authors: Nie Weidan , Liu Lili , Chen Yinxia , Luo Mingyin , Feng Chenghao , Wang Chaonan , Yang Zhongmin , Du Chong
Published: June 2023

Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (Mi-3/Mi-3), was treated at different soil temperatures before RNA-seq, and six differentially expressed genes (DEGs) encoding WRKY proteins were screened. In this study, cloning and sequencing were used to identify six target DEGs encoding SlWRKY1, SlWRKY13, SlWRKY30, SlWRKY41, SlWRKY46, and SlWRKY80. Conserved domain identification and phylogenetic tree analysis showed that SlWRKY1, SlWRKY13, and SlWRKY46 have similar functions and are mainly involved in plant growth and development and abiotic stress responses. SlWRKY30 and SlWRKY41 share high homology, while AtWRKY46 and AtWRKY70, which are highly homologous to SlWRKY80, play an important role in the disease resistance of A. thaliana. Considering these findings combined with the high level of SlWRKY80 expression observed in the roots and leaves of the resistant variety Motelle (Mi-1/Mi-1) and the continuous upregulation of SlWRKY80 expression in the roots after inoculation of Motelle with M. incognita, it is speculated that SlWRKY80 plays an important role in the Mi-1-mediated disease resistance pathway. Further study revealed that SlWRKY80 is a typical nuclear-localized protein, and a virus-induced gene silencing (VIGS) assay verified that SlWRKY80 is involved in tomato resistance to RKNs as a positive regulator. SA and JA signals play an important role in Mi-1-mediated resistance to RKNs. SlWRKY80 was able to respond rapidly to treatment with both plant hormones, which indicated that SlWRKY80 might be involved in disease resistance regulation through various immune pathways.
Grading Criteria of Anthurium DUS Quantitative Characteristics by Multiple Comparison
Authors: Chu Yunxia , Ren Li , Deng Shan , Li Shouguo , Zhang Yiying , Chen Hairong
Published: June 2023

The determination of the grades and interval of quantitative characteristics is an important job while we draft new distinctness, uniformity and stability (DUS) test guidelines. Grading criteria should be adjusted because of the effect of year and site; it is also a key task to establish applicable criteria in the DUS test. Excellent criteria will improve the accuracy of the DUS evaluation. In this study, we analyzed the variability and distribution patterns of nine quantitative characteristics of 251 anthurium varieties. Three methods were used to establish the grade criteria: the two standard deviation methods, the two LSD0.05 methods and the multiple comparison method. The results showed that the coefficient of variation within varieties varied from 6.96% to 10.11%. The quantitative characteristics observed in this study did not follow a normal distribution, except spadix thickness at the middle and spathe size. In most characteristics, the standard deviations and LSD0.05 were similar, except for spathe size. The state interval set by multiple comparison methods for every characteristic was variable, and its mean was about 1.25 times that of the other two methods. The process of establishing grading criteria using the multiple comparison method was simpler, and the criteria were more accurate, with a lower error rate.
Optimized Peppermint Essential Oil Microcapsules Loaded into Gelatin-Based Cryogels with Enhanced Antimicrobial Activity
Authors: Demir Didem , Goksen Gulden , Ceylan Seda , Trif Monica , Rusu Alexandru Vasile
Published: June 2023

In this study, chitosan (Chi) was used to microencapsulate peppermint essential oil (PEO). A novel gelatin-based cryogel loaded with PEO microcapsules was further developed and characterized for potential applications. Four different cryogel systems were designed, and the morphological, molecular, physical and antibacterial properties were investigated. Additionally, the antimicrobial properties of PEO, alone and microcapsulated, incorporated into the cryogel network were evaluated. The observed gel structure of cryogels exhibited a highly porous morphology in the microcapsules. The highest values of the equilibrium swelling ratio were acquired for the GelCryo–ChiCap and GelCryo–PEO@ChiCap samples. The contact angle GelCryo–PEO@ChiCap sample was lower than the control (GelCryo) due to the water repelling of the essential oil. It has been found that the incorporation of encapsulated PEO into the cryogels would be more advantageous compared to its direct addition. Moreover, GelCryo–PEO@ChiCap cryogels showed the strongest antibacterial activities, especially against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria). The system that was developed showed promising results, indicating an improved antibacterial efficacy and enhanced structural properties due to the presence of microcapsules. These findings suggest that the system may be an appropriate candidate for various applications, including, but not limited to, drug release, tissue engineering, and food packaging. Finally, this system demonstrates a strategy to stabilize the releasing of the volatile compounds for creating successful results.
Fault Diagnosis of Bearings Using Wavelet Packet Energy Spectrum and SSA-DBN
Authors: Qu Jinglei , Cheng Xueli , Liang Ping , Zheng Lulu , Ma Xiaojie
Published: June 2023

To enhance fault characteristics and improve fault detection accuracy in bearing vibration signals, this paper proposes a fault diagnosis method using a wavelet packet energy spectrum and an improved deep confidence network. Firstly, a wavelet packet transform decomposes the original vibration signal into different frequency bands, fully preserving the original signal’s frequency information, and constructs feature vectors by extracting the energy of sub-frequency bands via the energy spectrum to extract and enhance fault feature information. Secondly, to minimize the time-consuming manual parameter adjustment procedure and increase the diagnostic accuracy, the sparrow search algorithm–deep belief network method is proposed, which utilizes the sparrow search algorithm to optimize the hyperparameters of the deep belief networks and reduce the classification error rate. Finally, to verify the effectiveness of the method, the rolling bearing data from Casey Reserve University were selected for verification, and compared to other commonly used algorithms, the proposed method achieved 100% and 99.34% accuracy in two sets of comparative experiments. The experimental results demonstrate that this method has a high diagnostic rate and stability.
Effects of Nitrate Recycle on the Sludge Densification in Plug-Flow Bioreactors Fed with Real Domestic Wastewater
Authors: Wang Jie-Fu , An Zhao-Hui , Zhang Xue-Yao , Angelotti Bob , Brooks Matt , Wang Zhi-Wu
Published: June 2023

The impact of adding a modified Ludzack–Ettinger (MLE) configuration with Nitrate Recycle (NRCY) on continuous-flow aerobic granulation has yet to be explored. The potential negative effects of MLE on sludge densification include that: (1) bioflocs brought by NRCY could compete with granules in feast zones; and (2) carbon addition to anoxic zones could increase the system organic loading rates and lead to higher feast-to-famine ratios. Two pilot-scale plug flow reactor (PFR) systems fed with real domestic wastewater were set up onsite to test these hypotheses. The results showed that MLE configuration with NRCY could hinder the sludge granulation, but the hindrance could be alleviated by the NRCY location change which to some extent also compensates for the negative effect of higher feast-to-famine ratios due to carbon addition in MLE. This NRCY location change can be advantageous to drive sludge densification without a radical washout of the sludge inventory, and had no effects on the chemical oxygen demand (COD) and nitrogen removal efficiencies. The PFR pilot design for the MLE process with a modified NRCY location tested in this study could be developed as an alternative to hydrocyclones for full-scale, greenfield, continuous sludge densification applications.
Disturbance Observer-Based Terminal Sliding Mode Tracking Control for a Class of Nonlinear SISO Systems with Input Saturation
Authors: Zhang Qiang , Liu Ping , Chen Yu , Deng Quan , Tong Angxin
Published: June 2023

This paper focuses on the trajectory tracking control for general nonlinear single-input single-output (SISO) systems in which the output is not directly related to the control input. To address the tracking problem with the consideration of possible model uncertainty, external disturbance, and control input saturation, we employ the input-output feedback linearization technique and design a finite-time disturbance observer-based terminal sliding mode controller to improve the tracking performance and enhance the robustness. The stability analysis is carried out by using the Lyapunov method. To alleviate the chattering while achieving an acceptable control performance, a boundary layer method is adopted for the trade-off between the high-frequency control actions and the bounded unavoidable nonzero steady-state error. The proposed method is evaluated on the two typical nonlinear systems, which are fully linearizable and partially linearizable, respectively, and compared to the state-of-the-art method in terms of tracking and robustness through comprehensive numerical simulations. The results show that the proposed method not only renders the estimated disturbance error tends to be zero in finite time, but also has superiority in the fast reaction to disturbance and small tracking error without high-frequency chattering.
CO2 Capture and H2 Recovery Using a Hollow Fiber Membrane Contactor
Authors: Jeong Cheonwoo , Pandey Sadanand , Lee Dongcheol , Park SangHyeon , Baik Joon Hyun , Kim Joonwoo
Published: June 2023

In this study, hydrogen was recovered and purified by using a membrane contactor unit from CO2-rich gas without the use of any basic chemicals such as amines. The membrane operational parameters were adjusted to achieve high CO2 removal and H2 recovery. The effects of gas flow rate, pressure, gas composition (CO2/H2 ratio), pressure difference between liquid and gas, and gas/liquid ratio on CO2 removal and H2 recovery were investigated. Depending on the gas flow rate, the contact time between gas and liquid could be controlled, changing the absorption amounts of CO2 and H2. Regarding gas composition, an increase in the CO2/H2 ratio from 0.25 to 1 boosted H2 recovery. Furthermore, increasing the CO2/H2 ratio above 1 (from 1 to 3) generally reduced H2 recovery from 98.7% to 83%. Additionally, supplementation with the optimal amount of additive enhanced CO2 removal and H2 recovery. Thus, using a membrane contactor system results in high CO2 removal (82.7–93.5%) and H2 recovery (91.5–98.7%). Moreover, H2 production and separation can be performed in one system, implying that CO2 removal can be performed more efficiently by the membrane contactor. This study offers a new and promising route for producing high-purity H2 while removing CO2.
Understanding Complexity in the Role of Market Forces in the Construction of a Public Cultural Service System: Evidence from 435 Children’s Libraries in China
Authors: Lin Jinlong , Zhao Zhengxin , Chen Xiaoxiao
Published: June 2023

China’s public cultural service system transitioned from a centrally controlled model to a more complex one due to the gradual introduction of market forces. This change brought new challenges and opportunities, making the role of market forces a practical concern. By analyzing data from 282 public and 153 private children’s libraries in China, this study investigates how market forces compensate for the government’s capacity limitations in constructing public cultural service systems. Results show that market factors within the scope of our study do not negatively impact the system but instead promote synergy between government and market entities to meet children’s cultural needs. It is essential not to sever the role of the market from its interdependent relationship with the government, as this stance is based on unrealistic assessments of how policies function in practice, potentially leading to inadequate public cultural services. This study provides novel empirical evidence from China by confirming the interdependent relationship between the market and the government in constructing public cultural service systems and highlights the significance of applying complexity thinking. Overall, understanding the complexity of the role of market forces is essential for the construction of a robust and inclusive public cultural service system.
Transcriptomic Changes Induced by Low and High Concentrations of Heavy Metal Exposure in Ulva pertusa
Authors: Seo Do Yeon , Park Mira , Park Jeong-In , Kim Jang K. , Yum Seungshic , Kim Youn-Jung
Published: June 2023

The impact of sewage and wastewater pollution on marine ecosystems is of increasing concern due to the rapid accumulation of heavy metals in seaweeds inhabiting near-shore environments. Seaweeds can be severely damaged by heavy metals throughout their life cycles. Although the physiological and ecological effects of heavy metal exposure have been studied, there is limited research on their molecular responses. Ulva pertusa is a prevalent seaweed species in South Korea and is ecologically significant in coastal ecosystems. We utilized high-throughput RNA sequencing to analyze changes in the transcriptome profiles of U. pertusa under low concentrations of heavy metals (MPS) and high concentrations of copper (MPS-Cu) and cadmium (MPS-Cd). Differential gene expression analysis revealed that 53 (control vs. MPS), 27 (MPS vs. MPS-Cd), and 725 (MPS vs. MPS-Cu) genes were expressed differentially. Differentially expressed genes identified in our study included those with protective roles against oxidative stress and those involved in metal transport to the vacuole. Furthermore, exposure to heavy metal stress had a negative impact on the photosynthetic apparatus structural proteins of U. pertusa, resulting in photosynthetic inhibition. Moreover, exposure to high concentrations of copper resulted in the activation of carbon-related metabolism. These findings contribute to our understanding of the molecular mechanisms underlying heavy metal toxicity in U. pertusa.