bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved IgG antibody diagnostics of hypersensitivity pneumonitis and pulmonary mycoses by means of newly evaluated serum antibody ranges and frequencies using IgG ImmunoCAP™
Authors: Sennekamp Joachim , Lehmann Emilia , Joest Marcus
Published: May 2022

Background

The ranges of most human IgG antibodies against avian, microbial, and chemical antigens between their cut off values and their maximum values detected by IgG ImmunoCAP™ (Thermo Fisher Diagnostics, Freiburg, Germany) are not well known in pulmonary immune-mediated disorders. In addition, for many antigens it is not yet known how frequently their IgG antibodies appear in patients with these lung diseases. Therefore, we evaluated ranges and frequencies of these IgG antibodies.

Methods

The sera of 47,200 patients with suspected hypersensitivity pneumonitis (HP, extrinsic allergic alveolitis) or bronchopulmonary mycoses (mainly allergic bronchopulmonary aspergillosis [ABPA]), which were examined for 32 various IgG antibodies against birds, bacteria, molds, yeasts, and chemicals using the IgG ImmunoCAP™ assay, were evaluated retrospectively.

Results

We found a large spreading of the specific IgG ranges with maximum values from 26 mgA/l for Rhizopus nigricans up to 4640 mgA/l for pigeon. When the maximum values in the literature are also taken into account, the ranges of avian antibodies reach values up to 7280 mgA/l, the ranges of molds and yeasts up to 1707 mgA/l, of bacterial thermoactinomycetes up to 206 mgA/l, and of chemicals up to 139 mgA/l. The evaluated antibody ranges of the individual antigens can be used to decide whether a detected IgG antibody value is weakly, moderately or strongly positive. According to consistent evidence from numerous studies, a strongly positive antibody indicates HP or pulmonary mycosis more likely than a weakly positive antibody. It was found that the antigens of the highest maximum antibody levels—pigeon, budgerigar, parrot, Aspergillus fumigatus—are the causative agents of the most common immune-mediated lung diseases: bird breeder’s lung and pulmonary aspergillosis. Evaluation of the frequencies of eight major IgG antibodies of HP revealed the following rates: pigeon 28%, Aspergillus fumigatus 25%, budgerigar 23%, Penicillium chrysogenum 11%, Saccharopolyspora rectivirgula 7%, Acremonium kiliense 6%, Aureobasidium pullulans 5%, and Thermoactinomyces vulgaris 2%.

Conclusion

This study is the first to evaluate the ranges not only of avian and Aspergillus fumigatus antigens, as has been done up to now, but also of antibodies against 24 other environmental antigens. Quantification of IgG antibodies regarding their specific ranges can help to improve the serodiagnostics of immune-mediated lung diseases. In the lower ranges IgG antibodies are mainly physiological, while higher ranges correlate with the mentioned diseases as HP and ABPA/allergic bronchopulmonary mycoses (ABPM). The determined frequencies of the eight HP antibodies can be helpful in establishing HP screening tests.
Multi-objective Optimisation Using Fuzzy and Weighted Sum Approach for Natural Gas Dehydration with Consideration of Regional Climate
Authors: Kong Zong Yang , How Bing Shen , Mahmoud Ahmed , Sunarso Jaka
Published: May 2022

The majority of the existing simulation-based research works on natural gas dehydration via absorption using tri-ethylene glycol (TEG) have focused on solving single or bi-objective problems where most of the objectives are in conflict with one another. It was not until 2017 that multi-objective problems with conflicting nature have started gaining significant interest in this field, especially those involving 3 or more objectives. In this work, a multi-objective optimisation (MOO) framework was developed involving two different techniques, i.e. the fuzzy optimisation and the weighted sum approach, for handling different conflicting objectives in a natural gas dehydration process. The developed framework is straightforward, which can be applied by anyone effortlessly and can be easily extended to data from other literatures. Two different case studies, which involved bi- and tri-objectives, are given here to illustrate the efficacy of the developed framework for improving the sustainability and performance of the natural gas dehydration process. Relative to previous works without optimisation, the optimum results obtained here provide a compromised solution between different objectives. Using fuzzy optimisation in case 1, for example, increases the net profit by 0.2% and reduces the VOC emissions by 33% (i.e. better sustainability). Although the water dew point increases by 15%, it is still within the specification range and hydrate formation will not occur.
Gamification Strategies: A Characterization Using Formal Argumentation Theory
Authors: Guerrero Esteban , Kalmi Panu
Published: May 2022

Gamifiedsoftware applications are omnipresent in everyday life. The idea of using game design elements in non-game contexts to engage and motivate tasks has rapidly gained traction in the human–computer interaction and the psychology fields, but scarcely in the artificial intelligence (AI) research area. In this paper, we propose asoftware agentperspective of gamification elements to solve two specific problems: (1) areactiveperspective that gamification designers have for those gamified affordances, i.e. the visual cue (output) is only triggered by user interaction, and (2) a lack of formal treatment of gamified software, where strict characterization of software behavior as done in AI, guarantee that the information-based output follows the intended goal of the software. Our contributions presented in this paper are (1) two taxonomies of affordances based on the type of information that every element communicates, and the type of agency that is capable; (2) a framework to formalize the decision-making process for gamified software agents; and (3) a characterization of gamifiedstoriesusingformal argumentation theorydialogues. We exemplify our contributions with two gamified platforms in the healthcare and financial literacy fields.
LISSU: Continuous Monitoring of SOA Communication with Constraint-Based Validation
Authors: Theissen-Lipp Johannes , Kröger Moritz , Heinrichs Benedikt , Decker Stefan
Published: May 2022

Service-oriented architectures (SOA) are becoming more widespread in the context of Industry 4.0, and their interface descriptions enable modular and scalable communication systems. Since syntactic checks such as data types are solved nowadays, the purpose of this work is to add semantic validation based on the idea of Semantic Web Services. This paper proposesLightweight Semantic Web Services for Units(LISSU) and integrates promising concepts from the Semantic Web into SOA. We complement existing syntactic checks with semantic ones (e.g. for units), extend one-time initial checks with continuous monitoring, and include expressive constrain-based validations. LISSU can be integrated into any SOA and significantly increases the predictability of communications. Before components communicate, it checks their semantics via ontology URIs and automatically converts units if possible. Continuous monitoring at runtime extracts sent messages and guarantees flawless data quality via constraint-based validations. A real-world demonstrator setup in the manufacturing domain proves effectiveness and practicality. We present LISSU, which integrates concepts from the Semantic Web into SOA setups. It enables a wide range of semantic validations before and during communication, thereby increasing the quality and predictability of SOA communication.
Policies for Material Circularity: the Case of Lithium
Authors: Roa Diana , Rosendahl Knut Einar
Published: May 2022

Improper waste management carries social risks and dissipates high-value materials. Moreover, material market prices do not reflect these hidden costs and values. Two important questions are how prices can inform society about their resource use impact and how market-based policies optimize material circularity. This study adds to the literature by analyzing the effect of market-based policies aimed at promoting circular material reuse in a market defied by harmful waste but enhanced by recycling. The findings indicate that a landfill tax is a first-best policy since it targets the external costs of waste disposal, improves welfare, reduces damages, and boosts recycling. If a landfill tax is not feasible, other programs like taxes, subsidies, and a tax-subsidy scheme provide second-best results. Remarkably, recycling subsidies can stimulate higher raw material extraction and generate rebound effects. We also explore other non-market-based strategies to prevent waste and make recycling more cost-competitive and easier to recycle. The numerical results and sensitivity analysis of the lithium market illustrate the model's flexibility and prove why some policies are superior to others for reducing waste and creating value from used materials. Our study results serve as a guide to designing policies for optimal material circularity.
Advances and outlook of TE-PCM system: a review
Authors: Liu Anbang , Xie Huaqing , Wu Zihua , Wang Yuanyuan
Published: May 2022

This review reports the most recent developments of thermoelectric (TE) system coupled with phase change material (PCM) and its promising integration options within various PCM deployment and structure design. These innovative TE coupled with PCM (TE-PCM) systems provide heat/cold energy with additional electric power which implies better harnessing of multiform energy. Fundamentals of TE-PCM system including thermoelectric effect are presented along with a basic mathematical formulation of the physical problem. The classification principles and configuration types of such systems are also summarized. The most representative studies related to the utilization of TE-PCM system in diversified application scenarios and their compatibility with other energy systems have been comprehensively reviewed and analyzed, including the component and structure optimization. In-depth analysis of the main technical and operational challenges in the future has been carried out, and the prospective development of more efficient TE-PCM system and its hybrid configurations are projected based on the current technological level.
Anthropocene Geochemical and Technological Signatures of an Experimental Landfill Bioreactor in the Central Valley of California
Authors: Barfod Gry H. , Seiser Reinhard , Yazdani Ramin , Wang Li , Lesher Charles E. , Jenkins Bryan M. , Thy Peter
Published: May 2022

The geochemical signatures of a 12-year-old experimental bioreactor at a California landfill are used to identify elemental concentrations and ratios that characterize the landfill and relate it to the age and state of technology of the deposited waste. The bioreactor was constructed and sealed with a synthetic liner during 2001–2002 and operated and monitored as an anaerobic digester to enhance methane production. In 2013, the bioreactor was sampled and trace element concentrations of the extracted fine fractions were determined. The concentrations normalized to a regional soil composition, reveal systematic peaks for transition metals, alkali metals, heavy metals, and various metalloids and non-metals. A group of potential solder elements (Cu, Zn, Cd, In, Sn, Pb, Bi, and Sb) shows moderate to strong co-variations and is largely attributed to household electronic components and other similar products, while elements that correlated well with rare-earth and other elements are related to the diluting effect of a soil component used as cover. Batteries show modest to little effects on the overall concentrations. Circulating fluids (recycled leachate) in the controlled reactor did not completely redistribute and homogenize the elemental signatures within the time frame of the bioreactor. It is concluded that the present experimental landfill defines an Anthropocene marker identifiable by building material (plaster), PVC plastic, and household electronic components (Pb–Sn solder). These marker elements and ratios are variably diluted by soil components identified by alkali metals, rare-earths, and high field-strength elements (Hf, Zr, Nb, and Ta).
Understanding Cancer
Authors: Hesketh Robin
Published: May 2022

One in two of us will develop cancer at some point in our lives and yet many of us don't understand how cancers arise. How many different kinds of cancer are there? What treatments are available? What does the future hold in terms of developing new therapies? This book demystifies cancer by explaining the underlying cell and molecular biology in a clear and accessible style. It answers the questions commonly asked about cancer such as what causes cancer and how cancer develops. It explains how DNA makes proteins and how mutations can corrupt those proteins. It also gives an overview of current therapies and how treatments may advance over the next decades, as well as explaining what actions we can take to help prevent cancer developing. Understanding Cancer is an accessible and engaging introduction to cancer biology for any interested reader.
Aristotle's Ethics
Authors: Gottlieb Paula
Published: May 2022

This Element is an examination of the philosophical themes presented in Aristotle's Nicomachean and Eudemian Ethics. Topics include happiness, the voluntary and choice, the doctrine of the mean, particular virtues of character and temperamental means, virtues of thought, akrasia, pleasure, friendship, and luck. Special attention has been paid to Aristotle's treatment of virtues of character and thought and their relation to happiness, the reason why Aristotle is the quintessential virtue ethicist. The virtues of character have not received the attention they deserve in most discussions of the relationship between the two treatises.
Research and modeling of force fighting equalisation for aircraft rudder’s triple active actuation system
Authors: Li D.W. , Lin M.X. , Tian L.
Published: May 2022

This paper presents a new approach to force fighting equalisation in a redundant active-active-active rudder actuation system that is used for the primary flight control system of a turboprop regional aircraft. The related coupled problem of force fighting scenario, and the hydraulic architecture of electronic-hydrostatic actuator (EHA) are analysed, the mathematical model of the EHA system is built. The virtual test bench is designed to evaluate the performance of the force fighting equalisation strategy. The proposed methodology is tested on an iron bird test rig. The physical experiment shows that the fighting force is minimised under all flight conditions, meets the low cost requirement and can be a very reliable system. The proposed methodology can be applied to other types of aircraft’ flight actuation systems.
The Potential of Arbitration as Effective Remedy in Business and Human Rights: Will the Hague Rules be Enough?
Authors: Baaij Andi
Published: May 2022

The area of business and human rights (BHR) has a gap in its means to effectively remedy human rights violations. In pursuit of implementing the third pillar of the UN Guiding Principles on Business and Human Rights, which focuses on providing an effective remedy to rightsholders affected by corporate human rights violations, it has been proposed to utilize arbitration as a new platform to deal with such violations. The Drafting Team that instigated this initiative has prepared a set of procedural rules for BHR arbitration, called the Hague Rules. The Rules were officially launched on 12 December 2019. These arbitral rules are tailored to the specific needs of settling human rights disputes. In this article, the general idea of BHR arbitration will be analysed and assessed in light of the normative concept of ‘effective’ remedy, using the Hague Rules as a focus point. This article will discuss not only what the Hague Rules would introduce to the general concept of BHR arbitration, but also what limitations might still remain in securing an effective remedy.
The importance of anthropology in psychiatry
Authors: Alexander L. , Lyne J. , Cannon M. , Roch B. A.
Published: May 2022

Anthropology is defined as “the study of humans”, while psychiatric anthropology is a subfield of cultural anthropology which uses qualitative methodologies to explore the experience of mental illness. In a field that is often dominated by quantitative research, an anthropological approach allows us to understand experiences surrounding illness and the cultural context of mental illness. The articles presented in this issue of the Irish Journal of Psychological Medicine explore individual and group perspectives within a variety of cultural and historical contexts. This compilation of articles unearths fascinating insights into the lived experiences of distinct and vulnerable groups, including young people, migrants and members of the travelling community. Harnessing these insights can help us to tailor our services to the needs of societal populations, as well as improving therapeutic relationships with the ultimate goal of better treatment outcomes.
How Knowledge Services Clustered Firms Leverage Different Channels of Local Knowledge Spillovers for Service Innovation
Authors: Ngo Thi (Alice) N. B. , Thornton Sabrina
Published: May 2022

Built upon configuration theory, this study performs a Qualitative Comparative Analysis (QCA) to delineate alternative and sufficient configurations of local knowledge spillover (LKS) channels, that is, how informal interactions and spinoff, and absorptive capacity are combined to facilitate service innovation. Primary data was collected from the largest software outsourcing cluster in Vietnam in 2018, which provides a sample size of 42 firms. The findings imply that multiple configurations of different channels of LKS in conjunction with absorptive capacity can lead to service innovation. This study makes three important contributions. First, it contributes to the debate over the critical role of LKS and absorptive capacity in innovation by offering a more holistic, yet nuanced understanding of the causal mechanisms underlying service innovation. Second, this study sheds light on viable and equifinal pathways for enhancing innovation capabilities, therefore contributing to the literature on cluster upgrading and global service sourcing. Third, it provides some managerial implications for indigenous spinoff firms in developing countries seeking to innovate through the strategic use of LKS.
Dietary inflammatory index, inflammation biomarkers and preeclampsia risk: a hospital-based case–control study
Authors: Liu Yan-hua , Zheng Lu , Cheng Chen , Li Shu-na , Shivappa Nitin , Hebert James R. , Fu Wen-jun , Zhao Xian-lan , Cao Yuan , Dou Wei-feng , Chen Hua-nan , Duan Dan-dan , Lyu Quan-jun , Zeng Fang-fang
Published: May 2022

This study evaluated the association between inflammatory diets as measured by the Dietary Inflammatory index (DII), inflammation biomarkers and the development of preeclampsia among the Chinese population. We followed the reporting guidelines of the Strengthening the Reporting of Observational Studies in Epidemiology statement for observational studies. A total of 466 preeclampsia cases aged over 18 years were recruited between March 2016 and June 2019, and 466 healthy controls were 1:1 ratio matched by age (±3 years), week of gestation (±1 week) and gestational diabetes mellitus. The energy-adjusted DII (E-DII) was computed based on dietary intake assessed using a seventy-nine item semiquantitative FFQ. Inflammatory biomarkers were analysed by ELISA kits. The mean E-DII scores were −0·65 ± 1·58 for cases and −1·19 ± 1·47 for controls (P value < 0·001). E-DII scores positively correlated with interferon-γ (rs = 0·194, P value = 0·001) and IL-4 (rs = 0·135, P value = 0·021). After multivariable adjustment, E-DII scores were positively related to preeclampsia risk (Ptrend < 0·001). The highest tertile of E-DII was 2·18 times the lowest tertiles (95 % CI = 1·52, 3·13). The odds of preeclampsia increased by 30 % (95 % CI = 18 %, 43 %, P value < 0·001) for each E-DII score increase. The preeclampsia risk was positively associated with IL-2 (OR = 1·07, 95 % CI = 1·03, 1·11), IL-4 (OR = 1·26, 95 % CI = 1·03, 1·54) and transforming growth factor beta (TGF-β) (OR = 1·17, 95 % CI = 1·06, 1·29). Therefore, proinflammatory diets, corresponding to higher IL-2, IL-4 and TGF-β levels, were associated with increased preeclampsia risk.
Achieving Groundwater Governance: Ostrom's Design Principles and Payments for Ecosystem Services Approaches
Authors: Nsoh Walters
Published: May 2022

Groundwater is a largely unseen common pool resource. Yet, driven by strong economic incentives, whether or not encouraged by existing policies, and the difficulty to exclude others, groundwater users are competing with each other to extract as much as possible, with devastating consequences for its sustainability. The challenges faced for sustainably managing such common pool resources, on which people have established de facto individual rights, are manifold. However, creating a market for trades of some kind in ecosystem services associated with groundwater could actually enhance the protection of this critical resource on the basis that protection can benefit individual groundwater users economically as well as provide a broader public good. This article uses Elinor Ostrom's design principles as an analytical tool to examine how market-based approaches such as payments for ecosystem services (PES) fit with some of the governance models that could be used to protect and enhance groundwater as a common pool resource. It argues that while there are specific design challenges to be overcome, PES as an institutional tool can align with Ostrom's ideas for the governance of groundwater.
Sensitivity of the Tropical Cyclone Boundary Layer to Vertical Diffusion in a Turbulent Kinetic Energy‐Based Boundary Layer Parameterization Scheme at Gray‐Zone Resolution
Authors: Xu Hongxiong , Duan Yihong
Published: May 2022

The impact of eddy diffusivities in the Mellor–Yamada–Nakanishi–Niino (MYNN) boundary layer parameterization on the fine‐scale turbulence and intensity of tropical cyclone (TC) simulations at gray‐zone resolution was investigated through comparison with large‐eddy simulation (LES) and observations. A series of idealized experiments were performed with varying vertical mixing lengths using the Advanced Research version of the Weather Research and Forecasting model. For enhanced eddy diffusivity cases, the storm intensity was sensitive to the eddy diffusivities and reducing the overestimation of the eddy diffusivities caused a significant increase in intensity. For the reduced eddy diffusivity cases, the TC intensity was marginally influenced by the eddy diffusivities. However, the reduction in eddy diffusivities effectively reproduced the fine‐scale turbulence. The stronger the resolved fine‐scale turbulence that could be established, the more frictional tendencies near the location of maximum wind were provided to the TC, the closer it was to the reference LES and observations. The resolved turbulence also had consequences in terms of preventing the wind gradient from increasing, which was one of the primary reasons for the discrepancies in the nonlinear intensity and fine‐scale features. The results of this study demonstrate that the modification of eddy diffusivities in the MYNN scheme can measure or guide improvements in the simulation of TC boundary layer fine‐scale features and intensity when operating at the gray‐zone resolution.
A Modeling Approach for Addressing Sensitivity and Uncertainty of Estuarine Greenhouse Gas (CO2 and CH4) Dynamics
Authors: Huang Peisheng , De Sousa Eduardo R. , Wells Naomi S. , Eyre Bradley D. , Gibbes Badin , Hipsey Matthew R.
Published: May 2022

Estuaries make an important contribution to the global greenhouse gas budget. Yet modeling predictions of carbon dioxide (CO2) and methane (CH4) emissions from estuaries remain highly uncertain due to both simplified assumptions about the underpinning hydrologic and biologic processes and inadequate data availability to uniquely define parameters related to CO2 and CH4 processes. This study presents a modeling framework to quantify the sensitivity and uncertainty of predicted CO2 and CH4 concentrations and emissions, which is demonstrated through application to a subtropical urban estuary (Brisbane River, Australia). A 3D hydrodynamic‐biogeochemical model was constructed, and calibrated using the model‐independent Parameter ESTimation software (PEST) with field data sets that captured strong gradients of CO2 and CH4 concentrations and emissions along the estuary. The approach refined uncertainty in the estimation of whole‐estuary annual emissions, and enabled us to assess the sensitivity and uncertainty of CO2 and CH4 dynamics. Estuarine CO2 concentrations were most sensitive to uncertainty in riverine inputs, whereas estuarine CH4 concentrations were most sensitive to sediment production and pelagic oxidation. Over the modeled year, variance in the daily fluctuations in carbon emissions from this case‐study spanned the full range of emission rates reported for estuaries around the world, highlighting that spatially or temporally limited sampling regimes could significantly bias estuarine greenhouse gas emission estimates. The combination of targeted field campaigns with the modeling approach presented in this study can help to improve carbon budgeting in estuaries, reduce uncertainty in emission estimates, and support management strategies to reduce or offset estuary greenhouse gas emissions.
Modeling Iceberg Longevity and Distribution During Heinrich Events
Authors: Fendrock Michaela , Condron Alan , McGee David
Published: May 2022

During the last glacial period (120–12 ka), the Laurentide ice sheet discharged large numbers of icebergs into the North Atlantic. These icebergs carried sediments that were dropped as the icebergs melted, leaving a record of past iceberg activity on the floor of the subpolar North Atlantic. Periods of significant iceberg discharge and increased ice‐rafted debris (IRD) deposition, are known as Heinrich Events. These events coincide with global climate change, and the melt from the icebergs involved is frequently hypothesized to have contributed to these changes in climate by adding a significant volume of cold, fresh water to the North Atlantic. Using an iceberg model coupled with the Massachusetts Institute of Technology Global Circulation Model numerical circulation model, we explore the various factors controlling iceberg drift and rates of melt that influence the spatial patterns of IRD deposition during Heinrich Events. In addition to clarifying the influence of sea surface temperature and wind on the path of an armada of icebergs, we demonstrate that the same volume of ice can produce very different patterns of iceberg drift simply by altering the size of icebergs involved. We note also a significant difference in the seasonal locations of icebergs, influenced primarily by the changing winds, and show that the spatial patterns of IRD for Heinrich Event 1 most closely corresponds to where icebergs are located during the summer months. Consistent with proxy evidence, the ocean must be several degrees colder than temperatures estimated for the Last Glacial Maximum in order for icebergs to travel the distance implied by Heinrich Layers.
A Probabilistic Model for Classifying Temporary Rivers
Authors: Rehnberg Morgan
Published: May 2022

The model relies on measurable broad-scale attributes, increasing its flexibility for use in diverse environments.
Intranasal administration of a recombinant RBD vaccine induces long-term immunity against Omicron-included SARS-CoV-2 variants
Authors: Lei Hong , Alu Aqu , Yang Jingyun , Ren Wenyan , He Cai , Lan Tianxia , He Xuemei , Yang Li , Li Jiong , Wang Zhenling , Song Xiangrong , Wang Wei , Lu Guangwen , Wei Xiawei
Published: May 2022

The outbreak of coronavirus disease 2019 (COVID-19) has posed great threats to global health and economy. Several effective vaccines are available now, but additional booster immunization is required to retain or increase the immune responses owing to waning immunity and the emergency of new variant strains. The deficiency of intramuscularly delivered vaccines to induce mucosal immunity urged the development of mucosal vaccines. Here, we developed an adjuvanted intranasal RBD vaccine and monitored its long-term immunogenicity against both wild-type and mutant strains of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), including Omicron variants, in mice. Three-dose intranasal immunization with this vaccine induced and maintained high levels of neutralizing IgG antibodies in the sera for at least 1 year. Strong mucosal immunity was also provoked, including mucosal secretory IgA and lung-resident memory T cells (TRM). We also demonstrated that the long-term persistence of lung TRM cells is a consequence of local T-cell proliferation, rather than T-cell migration from lymph nodes. Our data suggested that the adjuvanted intranasal RBD vaccine is a promising vaccine candidate to establish robust, long-lasting, and broad protective immunity against SARS-CoV-2 both systemically and locally.
Blue-light fundus autofluorescence imaging of pigment epithelial detachments
Authors: Bindewald-Wittich Almut , Dolar-Szczasny Joanna , Kuenzel Sandrine H. , von der Emde Leon , Pfau Maximilian , Rejdak Robert , Schmitz-Valckenberg Steffen , Ach Thomas , Dreyhaupt Jens , Holz Frank G.
Published: May 2022

Background

Pigment epithelial detachments (PEDs) occur in association with various chorioretinal diseases. With respect to the broad clinical spectrum of PEDs we describe fundus autofluorescence (FAF) characteristics of PEDs.

Methods

Ninety-three eyes of 66 patients (mean age 71.9 ± 11.1) with uni- or bilateral PED ( ≥ 350 µm) were included in a retrospective cross-sectional study. PEDs were secondary to age-related macular degeneration (n = 79), central serous chorioretinopathy (n = 7), polypoidal choroidal vasculopathy (n = 2), pattern dystrophy (n = 3) or idiopathic PED (n = 2). FAF images were recorded using confocal scanning laser ophthalmoscopy (488 nm excitation wavelength, detection of emission >500 nm). Diagnosis of PED was confirmed using spectral-domain optical coherence tomography. A qualitative FAF grading system was established, and grading was performed by two independent readers.

Results

PEDs showed highly variable characteristics on FAF imaging. FAF within the area of PED was found to be irregular/granular (n = 59, 63.4%), increased (n = 28, 30.1%), decreased (n = 3, 3.2 %), or normal (n = 3, 3.2%). Accompanying FAF changes included condensation of macular pigment (n = 67, 72.0%), focally increased FAF at the PED apex (n = 14, 15.1%) or elsewhere (n = 52, 55.9%), focally decreased FAF (n = 23, 24.7%), a cartwheel-like pattern (n = 10, 10.8%), a doughnut sign (n = 6, 6.5%), and a halo of decreased FAF encircling the PED (completelyn = 20, 21.5% or incompletelyn = 20, 21.5%).

Conclusions

PEDs show a variety of abnormal patterns on FAF imaging. These changes in FAF signals may be secondary to morphological and metabolic alterations within corresponding retinal layers and do not necessarily correspond with the underlying PED subtype or a specific pathology.
A murine model of large-scale bone regeneration reveals a selective requirement for Sonic Hedgehog
Authors: Serowoky Maxwell A. , Kuwahara Stephanie T. , Liu Shuwan , Vakhshori Venus , Lieberman Jay R. , Mariani Francesca V.
Published: May 2022

Building and maintaining skeletal tissue requires the activity of skeletal stem and progenitor cells (SSPCs). Following injury, local pools of these SSPCs become active and coordinate to build new cartilage and bone tissues. While recent studies have identified specific markers for these SSPCs, how they become activated in different injury contexts is not well-understood. Here, using a model of large-scale rib bone regeneration in mice, we demonstrate that the growth factor, Sonic Hedgehog (SHH), is an early and essential driver of large-scale bone healing.Shhexpression is broadly upregulated in the first few days following rib bone resection, and conditional knockout ofShhat early but not late post-injury stages severely inhibits cartilage callus formation and later bone regeneration. Whereas Smoothened (Smo), a key transmembrane component of the Hh pathway, is required in Sox9+ lineage cells for rib regeneration, we find thatShhis required in aPrrx1-expressing, Sox9-negative mesenchymal population. Intriguingly, upregulation ofShhexpression and requirements forShhandSmomay be unique to large-scale injuries, as they are dispensable for both complete rib and femur fracture repair. In addition, single-cell RNA sequencing of callus tissue from animals with deficient Hedgehog signaling reveals a depletion ofCxcl12-expressing cells, which may indicate failed recruitment ofCxcl12-expressing SSPCs during the regenerative response. These results reveal a mechanism by whichShhexpression in the local injury environment unleashes large-scale regenerative abilities in the murine rib.
Using open-source data to construct 20 metre resolution maps of children’s travel time to the nearest health facility
Authors: Watmough Gary R. , Hagdorn Magnus , Brumhead Jodie , Seth Sohan , Delamónica Enrique , Haddon Charlotte , Smith William C.
Published: May 2022

Physical access to health facilities is an important factor in determining treatment seeking behaviour and has implications for targets within the Sustainable Development Goals, including the right to health. The increased availability of high-resolution land cover and road data from satellite imagery offers opportunities for fine-grained estimations of physical access which can support delivery planning through the provision of more realistic estimates of travel times. The data presented here is of travel time to health facilities in Uganda, Zimbabwe, Tanzania, and Mozambique. Travel times have been calculated for different facility types in each country such as Dispensaries, Health Centres, Clinics and Hospitals. Cost allocation surfaces and travel times are provided for child walking speeds but can be altered easily to account for adult walking speeds and motorised transport. With a focus on Uganda, we describe the data and method and provide the travel maps, software and intermediate datasets for Uganda, Tanzania, Zimbabwe and Mozambique.
Physiological mechanisms of ABA-induced salinity tolerance in leaves and roots of rice
Authors: Chen Guanjie , Zheng Dianfeng , Feng Naijie , Zhou Hang , Mu Dewei , Zhao Liming , Shen Xuefeng , Rao Gangshun , Meng Fengyan , Huang Anqi
Published: May 2022

Abscisic acid (ABA) plays a crucial role in response to abiotic stress as important small molecules in regulating metabolism. This study aimed to evaluate the ability of foliar spraying ABA to regulate growth quality at rice seedling stage under salt stress. Results demonstrated that salt stress strongly reduced all the growth parameters of two rice seedlings (‘Chaoyouqianhao’ and ‘Huanghuazhan’), caused prominent decrease in the levels of photosynthetic pigments (mainly in Huanghuazhan), photosynthesis and fluorescence parameters. Salinity treatment increased the concentration of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in roots, whereas significant decreased H2O2was found in leaves of Huanghuazhan. Additionally, salinity triggered high Na+content particularly in leaves and enhanced catalase (CAT) activities, ascorbate peroxidase (APX) and peroxidase (POD) activities of the two rice seedlings. Nevertheless, salinity-induced increased root ascorbic acid (AsA) and glutathione (GSH) levels while decreased in leaves, which depended on treatment time. Conversely, ABA application partially or completely mitigated salinity toxicity on the seedlings. ABA could reverse most of the changed physiological parameters triggered by salt stress. Specially, ABA treatment improved antioxidant enzyme levels and significantly reduced the Na+content of two varieties as well as increased the K+, Mg2+and Ca2+content in leaves and roots. ABA treatment increased the hormone contents of 1-aminocclopropane carboxylic acid (ACC), trans-zeatin (TZ), N6-isopentyladenosine (IPA), Indole-3-acetic acid (IAA), and ABA in leaves of two rice varieties under salt stress. It is suggested that ABA was beneficial to protect membrane lipid peroxidation, the modulation of antioxidant defense systems and endogenous hormonal balance with imposition to salt stress.
A monitoring survey and health risk assessment for pesticide residues on Codonopsis Radix in China
Authors: Wang Yanping , Han Jiabin , Zhang Jinjin , Li Xue , Bai Ruibin , Hu Fangdi
Published: May 2022

In recent years, the safety of Codonopsis Radix (CR) has attracted considerable attention. Pesticide residues is an important index to evaluate the safety of CR. The purpose of this study was to monitor pesticide residues in 164 batches of CR in China and assess dietary risk assessment. Firstly, a combined method of QuEChERS-GC–MS/MS and QuEChERS-LC–MS/MS was established for determination of 155 pesticide residues in CR. Second, 155 Pesticide residues in 3 CR cultivars from Gansu, Shanxi, Hubei, Guizhou and Chongqing were determined by this method. Finally, the risk score of pesticide residues in CR was evaluated, and the dietary health risk was evaluated based on the pesticide residues in CR. The results demonstrated that one or more pesticide residues were detected in 39 batches (23.78%) of 164 batches of CR. Of the 155 pesticide residues, 20 were detected. The most frequently detected pesticide residue was dimethomorph with a detection rate of 5.49%. Risk scores showed that 6 pesticides were at higher risk. Risk assessment based on the hazard quotient/hazard index (HQ/HI) approach revealed that exposure to pesticide residues which detected in CR were far below levels that might pose a health risk.
Natural extracts, honey, and propolis as human norovirus inhibitors
Authors: Ruoff Kerstin , Devant Jessica Michelle , Hansman Grant
Published: May 2022

Norovirus is the most important cause of acute gastroenteritis, yet there are still no antivirals, vaccines, or treatments available. Several studies have shown that norovirus-specific monoclonal antibodies, Nanobodies, and natural extracts might function as inhibitors. Therefore, the objective of this study was to determine the antiviral potential of additional natural extracts, honeys, and propolis samples. Norovirus GII.4 and GII.10 virus-like particles (VLPs) were treated with different natural samples and analyzed for their ability to block VLP binding to histo-blood group antigens (HBGAs), which are important norovirus co-factors. Of the 21 natural samples screened, date syrup and one propolis sample showed promising blocking potential. Dynamic light scattering indicated that VLPs treated with the date syrup and propolis caused particle aggregation, which was confirmed using electron microscopy. Several honey samples also showed weaker HBGA blocking potential. Taken together, our results found that natural samples might function as norovirus inhibitors.
Purification, characterization, and determination of biological activities of water-soluble polysaccharides from Mahonia bealei
Authors: Kakar Mohib Ullah , Li Jingyi , Mehboob Muhammad Zubair , Sami Rokayya , Benajiba Nada , Ahmed Aziz , Nazir Amina , Deng Yulin , Li Bo , Dai Rongji
Published: May 2022

Mahonia bealeiis one of the important members of the genus Mahonia and Traditional Chinese Medicine (TCM). Several compounds isolated from this plant have exhibited useful biological activities. Polysaccharides, an important biomacromolecule have been underexplored in case ofM. bealei. In this study, hot water extraction and ethanol precipitation were used for the extraction of polysaccharides from the stem ofM. bealei, and then extract was purified using ultrafiltration membrane at 50,000 Da cut off value. Characterization of the purifiedM. bealeipolysaccharide (MBP) was performed using Fourier Transform Infrared Spectroscopy (FT-IR), along with Scanning Electron Microscopy (SEM), X-ray crystallography XRD analysis and Thermal gravimetric analysis (TGA). The purified polysaccharide MBP was tested for antioxidant potential by determining its reducing power, besides determining the DPPH, ABTS, superoxide radical, and hydroxyl radical scavenging along with ferrous ion chelating activities. An increased antioxidant activity of the polysaccharide was reported with increase in concentration (0.5 to 5 mg/ml) for all the parameters. Antimicrobial potential was determined against gram positive and gram-negative bacteria. 20 µg/ml MBP was found appropriate with 12 h incubation period againstEscherichia coliandBacillus subtilisbacteria. We conclude that polysaccharides fromM. bealeipossess potential ability of biological importance; however, more studies are required for elucidation of their structure and useful activities.
Changes in smell and taste perception related to COVID-19 infection: a case–control study
Authors: Cattaneo Camilla , Pagliarini Ella , Mambrini Sara Paola , Tortorici Elena , Mené Roberto , Torlasco Camilla , Perger Elisa , Parati Gianfranco , Bertoli Simona
Published: May 2022

The main aim of the present study was to psychophysically evaluate smell and taste functions in hospitalized COVID-19 patients and to compare those results with a group of healthy subjects. Another aim of the study was to assess the relationship of changes in patients’ smell and taste functions with a number of clinical parameters, symptoms, and other physiological signs as well as with severity of disease. Olfactory and gustatory functions were tested in 61 hospitalized patients positive for SARS-CoV-2 infection and in a control group of 54 healthy individuals. Overall, we found a significant impairment of olfactory and gustatory functions in COVID-19 patients compared with the control group. Indeed, about 45% of patients self-reported complaints about or loss of either olfactory or gustatory functions. These results were confirmed by psychophysical testing, which showed a significantly reduced performance in terms of intensity perception and identification ability for both taste and smell functions in COVID-19 patients. Furthermore, gustatory and olfactory impairments tended to be more evident in male patients suffering from more severe respiratory failure (i.e., pneumonia with need of respiratory support need during hospitalization).
Anemia warrants treatment to improve survival in patients with heart failure receiving sacubitril–valsartan
Authors: Yang Tsun-Yu , Lee Chii-Ming , Wang Shih-Rong , Cheng Yu-Yang , Weng Shao-En , Hsu Wan-Tseng
Published: May 2022

Angiotensin inhibition remains a cornerstone for pharmacologic management of heart failure (HF), despite being associated with decreased hemoglobin (Hb) levels. To investigate the effect of anemia and its treatment on patients with HF treated with sacubitril–valsartan (S/V), we conducted a retrospective study involving patients with recorded left ventricular ejection fractions (LVEFs) of < 40% between January 2017 and December 2019. We identified 677 patients, 37.7% of whom received S/V. The median follow-up period was 868 days. Anemia was associated with significantly decreased survival, increased mortality rates, and higher all-cause hospitalizations in S/V-using patients. We further analyzed 236 patients with HF who had recorded renal function, LVEF, and Hb at the initiation of S/V therapy to identify Hb patterns after S/V therapy. Of these patients, 35.6% exhibited decreasing Hb 12 months after S/V initiation, which was associated with a lower survival rate. Among the patients who were not prescribed anemia medications, Hb of ≥ 12 (vs. < 12 g/dL) was associated with a higher survival rate; this association was absent among the patients undergoing anemia treatment. These results emphasize that consistent screening and treatment for anemia should be implemented to reduce the morbidity and mortality of patients with HF receiving S/V.
Cisterna chyli as an optimal marker of tolvaptan response in severe cirrhotic ascites
Authors: Hirooka Masashi , Koizumi Yohei , Yano Ryo , Nakamura Yoshiko , Sunago Koutarou , Yukimoto Atsushi , Watanabe Takao , Yoshida Osamu , Tokumoto Yoshio , Abe Masanori , Hiasa Yoichi
Published: May 2022

For patients with cirrhosis, no definitive predictor of the efficacy and prognosis of tolvaptan treatment exists. We assessed the cisterna chyli’s utility as an optimal marker. We retrospectively enrolled 172 patients with cirrhosis. The effect of tolvaptan was evaluated using post-treatment survival time. The overall response to tolvaptan was 52.3%. The median cisterna chyli diameter was 4.1 mm. Of 172 patients, 100 were included in the pilot set and 72 in the validation set. According to the Youden index, the cisterna chyli diameter’s cutoff value was 4 mm, with a sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio of 92%, 83%, 86%, 91%, 5.43, and 0.09, respectively, in the pilot set. The area under the curve of the cisterna chyli diameter for evaluating tolvaptan’s effect was 0.911 and 0.988 in the pilot and validation sets, respectively. During multivariate analysis, cisterna chyli narrowing and furosemide treatment were significant predictive factors for tolvaptan’s insufficient effect. Cumulative liver transplantation-free survival rates were significantly higher in patients with cisterna chyli dilatation than in those without (p = 0.028). Our findings suggest a strong association of cisterna chyli with tolvaptan treatment response in patients with cirrhosis and hepatic edema.
On the round number bias and wisdom of crowds in different response formats for numerical estimation
Authors: Honda Hidehito , Kagawa Rina , Shirasuna Masaru
Published: May 2022

When asked for numerical estimations, people can respond by stating their estimates (e.g., writing down a number) or indicating a number on a scale. Although these methods are logically the same, such differences may affect the responses to the numerical estimations. In this study, we examined how differences in response format affected responses to numerical estimations using two behavioral experiments. We found that participants showed a round number bias (i.e., people answered estimates with round numbers) when simply stating a number and the distribution of responses tended to be less diverse. In contrast, this tendency was not observed when the participants responded using a scale. Participants provided more diverse estimates when they answered using a scale. Furthermore, we analyzed how this difference in response distribution was related to the wisdom of crowds (the aggregated judgment is as accurate as, or sometimes better than, the best individual judgment in the group) using computer simulations. The results indicated that round number bias affected the achievement of the wisdom of crowds. Particularly, when the group size was small, biased responses resulted in less effective achievement. Our findings suggest that using an appropriate scale is a low-cost method for eliminating round number bias and efficiently achieving the wisdom of crowds.