bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and validation of nomograms for predicting overall survival and cancer specific survival in locally advanced breast cancer patients: A SEER population-based study
Authors: Yin Fangxu , Wang Song , Hou Chong , Zhang Yiyuan , Yang Zhenlin , Wang Xiaohong
Published: September 2022

Background

For patients with locally advanced breast cancer (LABC), conventional TNM staging is not accurate in predicting survival outcomes. The aim of this study was to develop two accurate survival prediction models to guide clinical decision making.

Methods

A retrospective analysis of 22,842 LABC patients was performed from 2010 to 2015 using the Surveillance, Epidemiology and End Results (SEER) database. An additional cohort of 200 patients from the Binzhou Medical University Hospital (BMUH) was analyzed. The least absolute shrinkage and selection operator (LASSO) regression was used to screen for variables. The identified variables were used to build a survival prediction model. The performance of the nomogram models was assessed based on the concordance index (C-index), calibration plot, receiver operating characteristic (ROC) curve, and decision curve analysis (DCA).

Results

The LASSO analysis identified 9 variables in patients with LABC, including age, marital status, Grade, histological type, T-stage, N-stage, surgery, radiotherapy, and chemotherapy. In the training cohort, the C-index of the nomogram in predicting the overall survival (OS) was 0.767 [95% confidence intervals (95% CI): 0.751–0.775], cancer specific survival (CSS) was 0.765 (95% CI: 0.756–0.774). In the external validation cohort, the C-index of the nomogram in predicting the OS was 0.858 (95% CI: 0.812–0.904), the CSS was 0.866 (95% CI: 0.817–0.915). In the training cohort, the area under the receiver operator characteristics curve (AUC) values of the nomogram in prediction of the 1, 3, and 5-year OS were 0.836 (95% CI: 0.821–0.851), 0.769 (95% CI: 0.759–0.780), and 0.750 (95% CI: 0.738–0.762), respectively. The AUC values for prediction of the 1, 3, and 5-year CSS were 0.829 (95% CI: 0.811–0.847), 0.769 (95% CI: 0.757–0.780), and 0.745 (95% CI: 0.732–0.758), respectively. Results of the C-index, ROC curve, and DCA demonstrated that the nomogram was more accurate in predicting the OS and CSS of patients compared with conventional TNM staging.

Conclusion

Two prediction models were developed and validated in this study which provided more accurate prediction of the OS and CSS in LABC patients than the TNM staging. The constructed models can be used for predicting survival outcomes and guide treatment plans for LABC patients.
Molecular classification of human papillomavirus-positive cervical cancers based on immune signature enrichment
Authors: Song Guanghui , Luo Jiangti , Zou Shaohan , Lou Fang , Zhang Tianfang , Zhu Xiaojun , Yang Jianhua , Wang Xiaosheng
Published: September 2022

Background

Human papillomavirus-positive (HPV+) cervical cancers are highly heterogeneous in clinical and molecular characteristics. Thus, an investigation into their heterogeneous immunological profiles is meaningful in providing both biological and clinical insights into this disease.

Methods

Based on the enrichment of 29 immune signatures, we discovered immune subtypes of HPV+ cervical cancers by hierarchical clustering. To explore whether this subtyping method is reproducible, we analyzed three bulk and one single cell transcriptomic datasets. We also compared clinical and molecular characteristics between the immune subtypes.

Results

Clustering analysis identified two immune subtypes of HPV+ cervical cancers: Immunity-H and Immunity-L, consistent in the four datasets. In comparisons with Immunity-L, Immunity-H displayed stronger immunity, more stromal contents, lower tumor purity, proliferation potential, intratumor heterogeneity and stemness, higher tumor mutation burden, more neoantigens, lower levels of copy number alterations, lower DNA repair activity, as well as better overall survival prognosis. Certain genes, such as MUC17, PCLO, and GOLGB1, showed significantly higher mutation rates in Immunity-L than in Immunity-H. 16 proteins were significantly upregulated in Immunity-H vs. Immunity-L, including Caspase-7, PREX1, Lck, C-Raf, PI3K-p85, Syk, 14-3-3_epsilon, STAT5-α, GATA3, Src_pY416, NDRG1_pT346, Notch1, PDK1_pS241, Bim, NF-kB-p65_pS536, and p53. Pathway analysis identified numerous immune-related pathways more highly enriched in Immunity-H vs. Immunity-L, including cytokine-cytokine receptor interaction, natural killer cell-mediated cytotoxicity, antigen processing and presentation, T/B cell receptor signaling, chemokine signaling, supporting the stronger antitumor immunity in Immunity-H vs. Immunity-L.

Conclusion

HPV+ cervical cancers are divided into two subgroups based on their immune signatures' enrichment. Both subgroups have markedly different tumor immunity, progression phenotypes, genomic features, and clinical outcomes. Our data offer novel perception in the tumor biology as well as clinical implications for HPV+ cervical cancer.
Frontline healthcare workers experiences and challenges with in-person and remote work during the COVID-19 pandemic: A qualitative study
Authors: Sims Holly , Alvarez Carmen , Grant Kimesha , Walczak Jessica , Cooper Lisa A. , Ibe Chidinma A.
Published: September 2022

Introduction

The COVID-19 pandemic created new and exacerbated existing stressors for frontline healthcare workers. Despite being disproportionately affected by COVID-19, little is known about the experiences of frontline healthcare workers serving marginalized populations in community settings.

Design

We used qualitative descriptive methods to understand the experiences of 12 frontline healthcare workers (HCWs) supporting primarily underserved populations in outpatient settings during COVID-19. Interviews were conducted from March to April 2021.

Methods

Interviews were held virtually via Zoom using a semi-structured interview guide. Interviews were audio-recorded, transcribed verbatim, and uploaded into NVivo 12 qualitative data analysis software. The transcripts were dually coded by members of the research team and a thematic analysis was conducted.

Results

Four major themes from the interviews were identified: stressors and burnout, coping strategies, organizational support, and recommendations. HCWs described how the early adjustment period to the pandemic created new challenges as they attempted to navigate changes in the workplace and altered responsibilities at home. HCWs felt largely unsupported by their organizations as they attempted to cope with stressors. Organizational support programs and resources often did not meet frontline workers' needs, and sentiments of unappreciation from leadership contributed to feelings of burnout and frustration as pandemic-related challenges persisted and evolved.

Conclusion

Despite encountering numerous stressors at work and home, resulting from pandemic-related disruptions, frontline HCWs continued to provide care for their clients while navigating emerging challenges. Health organizations should include HCWs in decision-making processes when implementing support systems for workers during times of crisis.
The relationship between COVID-19-related restrictions and fear of missing out, problematic smartphone use, and mental health in college students: The moderated moderation effect of resilience and social support
Authors: Gong Zhun , Lv Yun , Jiao Xinian , Liu Jinhang , Sun Yingjie , Qu Qunzhen
Published: September 2022

As one of the groups most affected by the epidemic, the mental health of college students during the epidemic is a focus of attention in multiple fields. Based on resource conservation theory, this study investigates the impact of COVID-19-related restrictions on college students' problematic smartphone use and mental health from two perspectives, students' individual factors and external environmental factors, and specifically explores the role of fear of missing out (FoMO), resilience and social support in this context. This study used a questionnaire method, and to control for common method bias, a multitemporal data collection strategy was used. The study used online questionnaire distribution, the final sample included 975 Chinese college students (497 males and 478 females), and of these, 10.3% were freshmen, 31.9% were sophomores, 31.6% were juniors, 12.3% were seniors, and 13.9% were postgraduates. The results of this study showed the following: (1) Perceived COVID-19-related strain was positively correlated with perceived FoMO, problematic smartphone use and mental health problems (depression, anxiety, stress) among college students. (2) FoMO partially mediated the relationship between perceived COVID-19-related restrictions and problematic smartphone use, and it fully mediated the relationship between perceived COVID-19-related restrictions and mental health problems. (3) Resilience and social support co-moderated the relationship between FoMO and problematic smartphone use or mental health problems (depression, anxiety, stress).
From past to future: Bibliometric analysis of global research productivity on nomogram (2000–2021)
Authors: Wang Xiaoxue , Lu Jingliang , Song Zixuan , Zhou Yangzi , Liu Tong , Zhang Dandan
Published: September 2022

Background

Nomogram, a visual clinical predictive model, provides a scientific basis for clinical decision making. Herein, we investigated 20 years of nomogram research responses, focusing on current and future trends and analytical challenges.

Methods

We mined data of scientific literature from the Core Collection of Web of Science, searching for the original articles with title “Nomogram*/Parton Table*/Parton Nomogram*”, published within January 1st, 2000 to December 30th, 2021. Data records were validated using HistCite Version and analyzed with a transformable statistical method, the Bibliometrix 3.0 package of R Studio.

Results

In total, 4,176 original articles written by 19,158 authors were included from 915 sources. Annually, Nomogram publications are continually produced, which have rapidly grown since 2018. China published the most articles; however, its total citations ranked second after the United States. Both total citations and average article citations in the United States rank first globally, and a high degree of cooperation exists between countries. Frontiers in Oncology published the most papers (238); this number has grown rapidly since 2019. Journal of Urology had the highest H-index, with an average increase in publications over the past 20 years. Most research topics were tumor-related, among which tumor risk prediction and prognostic evaluation were the main contents. Research on prognostic assessment is more published and advanced, while risk prediction and diagnosis have good developmental prospects. Furthermore, nomogram of the urinary system has been highly developed. Following advancements in nomogram modeling, it has recently been applied to non-oncological subjects.

Conclusion

This bibliometric analysis provides a comprehensive overview of the current nomogram status, which could enable better understanding of its development over the years, and provide global researchers a comprehensive analysis and structured information to help identify hot spots and gaps in future research.
Investigation of independent reinforcement learning algorithms in multi-agent environments
Authors: Lee Ken Ming , Ganapathi Subramanian Sriram , Crowley Mark
Published: September 2022

Independent reinforcement learning algorithms have no theoretical guarantees for finding the best policy in multi-agent settings. However, in practice, prior works have reported good performance with independent algorithms in some domains and bad performance in others. Moreover, a comprehensive study of the strengths and weaknesses of independent algorithms is lacking in the literature. In this paper, we carry out an empirical comparison of the performance of independent algorithms on seven PettingZoo environments that span the three main categories of multi-agent environments, i.e., cooperative, competitive, and mixed. For the cooperative setting, we show that independent algorithms can perform on par with multi-agent algorithms in fully-observable environments, while adding recurrence improves the learning of independent algorithms in partially-observable environments. In the competitive setting, independent algorithms can perform on par or better than multi-agent algorithms, even in more challenging environments. We also show that agents trained via independent algorithms learn to perform well individually, but fail to learn to cooperate with allies and compete with enemies in mixed environments.
Ischemic stroke can have a T1w hyperintense appearance in absence of intralesional hemorrhage
Authors: Weston Philippa , Behr Sebastien , Garosi Laurent , Maeso Christian , Carrera Ines
Published: September 2022

Magnetic resonance imaging (MRI) signal changes associated with ischemic stroke are typically described as T2w and FLAIR hyperintense, and T1w isointense lesions. Intralesional T1w hyperintensity is generally attributed to either a hemorrhagic stroke, or an ischemic stroke with hemorrhagic transition, and has an associated signal void on gradient echo (GE) sequences. Cases of ischemic stroke with T1w hyperintense signal in absence of associated signal void on GE sequences have been sporadically demonstrated in human stroke patients, as well as in dogs with experimentally induced ischemia of the middle cerebral artery. This multicenter retrospective descriptive study investigates the presence of T1w hyperintensity in canine stroke without associated signal void on GE sequences. High field (1.5 Tesla) MRI studies of 12 dogs with clinical presentation, MRI features, and cerebrospinal fluid results suggestive of non-hemorrhagic stroke were assessed. The time between the observed onset of clinical signs and MRI assessment was recorded. All 12 patients had an intralesional T1w hyperintense signal compared to gray and white matter, and absence of signal void on T2*w GE or SWI sequences. Intralesional T1w hyperintensities were either homogenously distributed throughout the entire lesion (6/12) or had a rim-like peripheral distribution (6/12). The mean time between the recorded onset of clinical signs and MRI assessment was 3 days; however, the age range of lesions with T1w hyperintense signal observed was 1–21days, suggesting that such signal intensities can be observed in acute, subacute, or chronic stages of ischemic stroke. Follow-up was recorded for 7/12 cases, all of which showed evidence of neurological improvement while in hospital, and survived to discharge. Correlation of the age and MRI appearance of lesions in this study with similar lesions observed in human and experimental studies suggests that these T1w hyperintensities are likely caused by partial tissue infarction or selective neuronal necrosis, providing an alternative differential for these T1w hyperintensities observed.
Gender-related differences in the effects of Inonotus obliquus polysaccharide on intestinal microorganisms in SD rats model
Authors: Hu Binhong , He Xinyue , Tan Jin , Ma Yichuan , Wang Gang , Liu Songqing , Li Mingyue , Guo Yanping , Sun Rong , Sun Mengxue , Deng Xin , Zhou WenJing , Lv Xue
Published: September 2022

Natural edible fungal polysaccharides are of research and application value for the prevention of diseases by improving the microenvironment within the intestine. Inonotus obliquus polysaccharide (IOP) extracts have strong antioxidant, anti-inflammatory, and other biological activities, and as such, it could be used as prebiotics to improve the viability of intestinal microbes, maintain intestinal homeostasis and improve intestinal immunity. The effects of sex on intestinal microbiota after IOP absorption was determined. In this study, IOP had different effects on the intestinal flora of male and female rats, with the diversity and richness showing opposite changes. At the same time, after IOP intervention, changes in the dominant intestinal flora of female rats was less compared with that of males. In addition, while Clostridia, Lactobacillus and Roseburia were the dominant intestinal microbes in female rats, males had mainly Bacteroidota from different families and genera, along with an increasing proportion of Muribaculaceae from different families and genera. IOP could further regulate the intestinal microenvironment of male and female SD rats by enhancing the vitality of their dominant microorganisms, and for both sexes, this enabled the screening of dominant microflora that were conducive to the balance of the intestinal flora. These results help to understand the effects of sex-related differences on the composition of the intestinal microbiota as well as on diseases.
Lactate uptake in the rumen and its contributions to subacute rumen acidosis of goats induced by high-grain diets
Authors: He Banglin , Fan Yaotian , Wang Hongrong
Published: September 2022

Rumen acidosis is the consequence of feeding rapidly fermentable grain diets and it is considered the most common nutritional disorder in intensive feeding ruminants. Due to that mechanism of catabolism and transformation is driven by multi-factors, the role of ruminal lactate and its contribution to subacute rumen acidosis has not been well defined yet. The aim of this study is to evaluate the effects of SARA on the production, absorption, circulation, and transformation of lactate in the rumen. In this study, rumen samples were collected from 12 adult Saanen goats (44.5 ± 4.6 kg BW) equipped with permanent rumen cannula to measure rumen fermentation parameters, organic acids production, microbial profiles, and blood indicators to identify the occurrence of SARA. To further investigate the change in the disappearance rate of ruminal lactate, rumen fluid was collected and a batch culture was performed. The results showed that the clearance rate of ruminal lactate was accelerated by SARA, and the concentration of the ruminal lactate pool was stable. In addition, the rumen liquid dilution rate and the rumen liquid flow rate under the SARA condition of goats were lower than that in normal conditions. The ruminal lactate flow rate had no difference throughout the process of fermentation. However, in vitro data showed that the disappearance of lactate was reduced in SARA. By measuring the conversion of sodium L-[3-13C]-lactate in batch culture, it was found that the percentage of lactate converted to propionate was significantly lower in the SARA treatment and 16.13% more lactate converted to butyrate under SARA condition. However, the percentage of lactate transformed into acetate and butyrate was significantly increased in the SARA treatment than that of control. The relative population of total protozoa count in SARA was significantly reduced, while the relative population of Lactobacillus fermentum, Streptococcus bovis, Butyrivibrio fibrisolvens, Megasphaera elsdenii, and Selenomonas ruminantium in the SARA treatment was significantly induced (p < 0.05). It is concluded that the transformation of lactate into butyrate may promote the development of SARA. These findings provide some references to the diet formulation for preventing SARA.
Kinetics and minimal inhibitory concentrations of ceftiofur in tear film following extended-release parenteral administration (Excede®) in dogs
Authors: Bowden Anna Catherine , Allbaugh Rachel A. , Smith Joe S. , Mochel Jonathan P. , Sebbag Lionel
Published: September 2022

Purpose

Describe the pharmacokinetics of extended-release parenteral ceftiofur (Excede®) in canine tear film and compare these concentrations to minimal inhibitory concentrations (MICs) of ceftiofur against common ocular pathogens in dogs.

Method

Six dogs of various breeds were enrolled. Disruption of blood-tear barrier was achieved with histamine-induced conjunctivitis to ensure clinical relevance of the results. Each dog received a single subcutaneous injection of 5 mg/kg Excede®, followed by tear collection with Schirmer strips at times 0, 0.25, 0.5, 1, 2, 4, 8, 12, 24, 36, 48, 72, 96, 120, 144, 168, 192, 216 and 240 h. Drug quantification was performed with liquid chromatography-mass spectrometry. MICs were determined for Staphylococcus pseudintermedius, Streptococcus canis and Pseudomonas aeruginosa by assessing bacterial growth (n = 10 per bacterial species) in the presence of ceftiofur at increasing concentrations.

Results

Blood-tear barrier breakdown provided tear film concentrations of ceftiofur 3.2–28.9-fold higher than in the contralateral healthy eye (n = 1 dog, pilot experiment). In all six dogs, ceftiofur concentrations in tears varied from 2.3 to 637.5 ng/mL and were detectable up to 10 days (240 h) after subcutaneous injection. However, tear levels always remained below MICs for common ocular isolates (≥640 ng/mL).

Conclusions

Ceftiofur reached the tear compartment (for up to 10 days) after a single parenteral injection, however tear concentrations were extremely variable and too low to be effective against common bacterial pathogens in dogs. Further studies with different ceftiofur dosage or other long-acting injectable antibiotics are warranted.
Dietary supplementation with β-mannanase and probiotics as a strategy to improve laying hen's welfare
Authors: Carvalho Camila Lopes , Andretta Ines , Galli Gabriela Miotto , Martins Gabriel Bueno , Camargo Nathalia de Oliveira Telesca , Stefanello Thais Bastos , Melchior Raquel , da Silva Marcos Kipper
Published: September 2022

A trend toward animal welfare improvement is observed in animal production, in addition to restrictions imposed on the use of antimicrobials. This study's objective was to evaluate whether β-mannanase and probiotic supplementation can change hen's behavior. Light weight laying hens (36 weeks old) were housed in cages randomly allocated to one of four different treatments: control group, fed non-supplemented diets; diets supplemented with 300 g/ton of β-mannanase; diets supplemented with 50 g/ton of probiotic; or diets containing both 300 g/ton of β-mannanase and 50 g/ton of probiotic. The behavior of 24 birds was recorded for a week using video cameras. The frequency and time of main behaviors (eating, walking, standing, sitting, drinking, and exploring) were analyzed in three periods per day (from 09:00 to 09:15; from 01:00 to 01:15, and from 04:00 to 04:15), as well as the time of other behaviors (leg-stretching and wings, scratching, wing-flapping, aggressive and non-aggressive pecks). Frequency and lesion scores were also analyzed using a visual score of three body regions: neck, tail, and cloaca; as well as comb injuries. β-mannanase was able to increase the frequency of feeding behavior by 49% (P < 0.05) and hens also spend 20% (P < 0.05) more time in this behavior compared to the control treatment. The use of probiotics also enhanced by 39% (P < 0.05) the frequency and 19% the time (P < 0.05) and the supplementation with combined additives was able to increase by 29% (P < 0.05) the frequency and 25% (P < 0.05) the time in feeding behavior. β-mannanase and probiotics also increased the frequency and time spent exploring behavior (P < 0.05) and promoted a higher frequency in standing behavior (P < 0.05) and decreased the time spent on sitting behaviors (P < 0.05). The combined additives showed less frequency and time in sitting behaviors (P < 0.05), while increased wing-flapping behavior (P < 0.05). All the treatments were able to reduce pecking (P < 0.05). Therefore, the addition of β-mannanase and probiotics to laying hen diets is an effective strategy to improve bird welfare.
The Aftermath of Long-Term Cigarette Smoking on Telomere Length and Mitochondrial DNA Copy Number in Human Cumulus Cells Prior to In Vitro Fertilization—A Pilot Study
Authors: Konstantinidou Fani , Budani Maria Cristina , Marconi Guya Diletta , Gonnella Francesca , Sarra Annalina , Trubiani Oriana , Stuppia Liborio , Tiboni Gian Mario , Gatta Valentina
Published: September 2022

Cigarette smoking among women of reproductive age is known to take a toll on systemic health and fertility potential by severely impacting ovarian tissues and cells, such as granulosa and cumulus cells (CCs). The purpose of this study was to determine the potential damage caused by tobacco smoke at a molecular level in the CCs of females who had undergone in vitro fertilization. The level of intracellular damage was determined by estimating the average telomere length (TL) and mitochondrial DNA copy number (mtDNA-CN), as well as the expression profile of telomere maintenance genes TERF1, TERF2, POT1 and microRNAs miR-155, miR-23a and miR-185. Western blotting analysis was performed to detect consequent protein levels of TERF1, TERF2 and POT1. Our results evidenced significantly lower relative TL and mtDNA-CN and a down-regulation pattern for all three described genes and corresponding proteins in the CCs of smokers compared with controls (p < 0.05). No significant differences were found in the miRNAs’ modulation. Combined, our data add another piece to the puzzle of the complex regulatory molecular networks controlling the general effects of tobacco smoke in CCs. This pilot study extends the until now modest number of studies simultaneously investigating the mtDNA-CN and TL pathways in the human CCs of smoking women.
The JAK1/2 Inhibitor Baricitinib Mitigates the Spike-Induced Inflammatory Response of Immune and Endothelial Cells In Vitro
Authors: Barilli Amelia , Visigalli Rossana , Ferrari Francesca , Recchia Luciani Giulia , Soli Maurizio , Dall’Asta Valeria , Rotoli Bianca Maria
Published: September 2022

The purpose of this study was to examine the effect of the JAK-STAT inhibitor baricitinib on the inflammatory response of human monocyte-derived macrophages (MDM) and endothelial cells upon exposure to the spike S1 protein from SARS-CoV-2. The effect of the drug has been evaluated on the release of cytokines and chemokines from spike-treated MDM, as well as on the activation of endothelial cells (HUVECs) after exposure to conditioned medium collected from spike-activated MDM. Results obtained indicate that, in MDM, baricitinib prevents the S1-dependent phosphorylation of STAT1 and STAT3, along with the induction of IP-10- and MCP-1 secretion; the release of IL-6 and TNFα is also reduced, while all other mediators tested (IL-1β, IL-8, RANTES, MIP-1α and MIP-1β) are not modified. Baricitinib is, instead, poorly effective on endothelial activation when HUVECs are exposed to supernatants from S1-activated macrophages; the induction of VCAM-1, indeed, is not affected by the drug, while that of ICAM-1 is only poorly inhibited. The drug, however, also exerts protective effects on the endothelium by limiting the expression of pro-inflammatory mediators, specifically IL-6, RANTES and IP-10. No effect of baricitinib has been observed on IL-8 synthesis and, consistently, on neutrophils chemiotaxis. Our in vitro findings reveal that the efficacy of baricitinib is limited, with effects mainly focused on the inhibition of the IL-6-mediated inflammatory loop.
Non-Cystic Fibrosis Bronchiectasis in Pediatric Age: A Case Series in a Metropolitan Area of Northern Italy
Authors: Gallucci Marcella , Candela Egidio , Di Palmo Emanuela , Miniaci Angela , Pession Andrea
Published: September 2022

Non-cystic fibrosis bronchiectasis is an emergent disease characterized by endobronchial suppuration, dilated airways with neutrophilic inflammation and chronic wet cough due to recurrent lower airway infections. A regular clinical follow-up and adequate management of exacerbations are essential to reduce symptoms and the worsening of lung injury. We report a retrospective study comprising 15 children and adolescents with NCFB followed in our hospital center of pediatric pulmonology. We retrospectively analyzed the main comorbidities associated with the presence of NCFB, the radiological aspect associated with the different etiologies and the therapeutic approach used. We also emphasized the importance of an effective preventive strategy to reduce and prevent pulmonary exacerbations.
An Annotated Checklist and the Conservation Status of Chondrichthyans in the Adriatic
Authors: Soldo Alen , Lipej Lovrenc
Published: September 2022

Although there is a high number of publications listing fish species in the Adriatic, only a few have focused on chondrichthyans, while their conservation status has been investigated even less. Thus, this paper aims to provide an updated and annotated checklist of the chondrichthyans occurring in the Adriatic waters with their presence and conservation status. Each species is evaluated against the criteria defined in IUCN (International Union for Conservation of Nature) Red List Categories and Criteria and according to the guidelines for national and regional level assessments. In total, 60 chondrichthyan species from 27 families and 42 genera are listed. The list contains 33 species of sharks, 26 species of rays and one chimera. Assessment of the conservation status reveals that three species are now considered Regionally Extinct (namely Squatina oculata, Pristis pectinata and Rhinobatos rhinobatos). A total of 21 species are assessed as Critically Endangered, 8 are Endangered and 10 are Vulnerable. Of the remaining species, six are Near Threatened and the same number of species are Least Concern and Data Deficient. Considering that the principal driver of chondrichthyan decline and regional extinction is overfishing, it is recommended that the Adriatic countries adopt the same management measures and strengthen their coordination.
Effect of Consumption Value on Consumer Willingness to Consume GM Food: A Post-COVID-19 Analysis
Authors: Ghufran Muhammad , Ashraf Jawaria , Ali Sumran , Xiaobao Peng , Aldieri Luigi
Published: September 2022

In this research, we debate the critical challenges posed by the COVID-19 pandemic such as food scarcity, by examining the influence of consumption values on consumers’ willingness to consume genetically modified (GM) food in the presence of consumer food attitudes, animosity, and ethnocentrism, which could be the one possible option to deal with the food scarcity problem. The proposed relationship could help to understand the complex societal problem of food scarcity and import dependency in the food sector before and after the crisis. Therefore, based on the theory of consumption values, we investigated government actions, consumer attitudes, and their willingness to consume GM food through 1340 valid USA responses and 1065 Chinese responses. We observed that COVID-19 doubled the number of malnourished people in 2020 relative to 2019, while consumption values, to some extent, changed consumer food attitudes and were inclined toward other food alternatives such as GM food regardless of governmental support for GM food in both USA and China. Moreover, this research enables governments, policymakers, market practitioners, and other stakeholders to use the COVID-19 crisis as an opportunity to negotiate with other countries to share their food technology along with imports.
Effects of Extraction Methods on Physicochemical and Structural Properties of Common Vetch Starch
Authors: Zhang Xiaojun , Cheng Yongqiang , Jia Xin , Geng Donghui , Bian Xiaojia , Tang Ning
Published: September 2022

Three extraction methods: water extraction, lactic acid bacteria fermentation, and back-slopping fermentation were applied to extract a new type of legume starch, common vetch starch. Our results showed that the lactic acid bacteria fermented starch had the highest amylose content (35.69%), followed by the back-slopping fermented starch (32.34%), and the water-extracted starch (30.25%). Furthermore, erosion surface, lower molecular weight, smaller particle size, larger specific surface area, and a higher proportion of B1 chain were observed in the fermented starch, especially in the back-slopping fermented starch. All the extracted starches showed a type C structure, but a type CB structure was observed in the back-slopping fermented starch. In addition, the relative crystallinity of the lactic acid bacteria fermented starch (34.16%) and the back-slopping fermented starch (39.43%) was significantly higher than that of the water-extracted starch (30.22%). Moreover, the swelling power, solubility, pasting, and thermal properties of the fermented starches were also improved. In conclusion, the fermentation extraction method, especially back-slopping fermentation, could improve the quality of the extracted common vetch starch when compared with the traditional water extraction method.
Characterization of Volatile Compounds by HS-GC-IMS and Chemical Composition Analysis of Colored Highland Barley Roasted at Different Temperatures
Authors: Wang Cong , Zhang Zhiming , Zhang Xiayin , Tian Xinyi , Chen Kai , Zeng Xiaoxiong
Published: September 2022

Colored highland barley (CHB) is featured with its potential health-promoting benefits. CHB is frequently processed through roasting, which changes its volatile smells, color, and composition. The objective of this work was to establish the volatile fingerprints of CHB that had been roasted at different temperatures using E-nose and headspace-gas-chromatography-ion-mobility spectroscopy (HS-GC-IMS). The findings showed that roasting increased the relative contents of pyrazines, aldehydes, and ketones while decreasing the relative contents of alcohols, esters, and sulfides. Pyrazines were identified as the markers for volatile substances of the roasted CHB (RCHB). The outcomes of the principal component analysis (PCA) and hierarchical clustering analysis (HCA) demonstrated that the volatiles could easily distinguish between raw CHB and RCHB instead of differentiating between CHB roasted at different temperatures. Additionally, after roasting, the color characteristics and CHB constituents underwent changes, and the effect of roasting temperature on these changes differed depending on the cultivar. Protein, free amino acids, and flavonoids appeared to primarily participate in the variations of volatile substances, and the free fluorescence intermediary compounds might involve changes in color parameters and aromas. These findings improved our knowledge of the volatiles in CHB that were roasted under various conditions.
Biological and Clinical Aspects on the Treatment of Schizophrenia and Related Disorders: New Challenges
Authors: Monreal José Antonio , González-Rodríguez Alexandre
Published: September 2022

For several decades, it has been postulated that dopaminergic pathways explain the neurobiology of schizophrenia, the biological underpinnings of treatment responses and the main mechanisms of action of antipsychotics [...]
Lifestyle in Obese Individuals during the COVID-19 Pandemic
Authors: Pavone Giovanna , Tartaglia Nicola , De Fazio Michele , Monda Vincenzo , Valenzano Anna , Cibelli Giuseppe , Polito Rita , Torre Maria Ester La , Scattarella Fabio , Mosca Laura , Scarinci Alessia , Martines Gennaro , Pacilli Mario , Messina Giovanni , Monda Marcellino , Messina Antonietta , Ambrosi Antonio
Published: September 2022

Background: Stay-at-home orders in response to the Coronavirus 2 (SARS-CoV-2) pandemic have forced abrupt changes to daily routines. The aim of this study is to describe the behavior of lifestyles of individuals with obesity on the waiting list for bariatric surgery in the Department of Medical and Surgical Sciences of University of Foggia during the COVID-19 pandemic. Materials and methods: From June 2020 to December 2020 an online survey format was administered to all the patients (n = 52) enrolled for bariatric surgery subjects with obesity, to obtain information about the COVID-19 pandemic’s impact on patients with obesity starting 9 March 2020 until 18 May 2020. Results: Our data showed that 58% of patients stated that the pandemic negatively affected their mood, 60% of patients confirmed that they changed their dietary behaviors during the stay-at-home period, as they consumed more unhealthy foods or spent less time cooking home cooked meals. In addition, 71% of patients stated that the closure of the gyms worsened their obesity condition and their mental well-being with an increase of a feeling of anxiety. Conclusions: Results showed that the COVID-19 pandemic has had a significant impact on health behaviors, including quality of life, mental health physical activity, weight maintenance, and consumption of sweets in obese patients.
Readmission of Patients to Acute Psychiatric Hospitals: Influential Factors and Interventions to Reduce Psychiatric Readmission Rates
Authors: Owusu Ernest , Oluwasina Folajinmi , Nkire Nnamdi , Lawal Mobolaji A. , Agyapong Vincent I. O.
Published: September 2022

Background: Appropriate and adequate treatment of psychiatric conditions in the community or at first presentation to the hospital may prevent rehospitalization. Information about hospital readmission factors may help to reduce readmission rates. This scoping review sought to examine the readmission of patients to acute psychiatric hospitals to determine predictors and interventions to reduce psychiatric readmission rates. Method: A scoping review was conducted in eleven bibliographic databases to identify the relevant peer-reviewed studies. Two reviewers independently assessed full-text articles, and a screening process was undertaken to identify studies for inclusion in the review. PRISMA checklist was adopted, and with the Covidence software, 75 articles were eligible for review. Data extraction was conducted, collated, summarized, and findings reported. Result: 75 articles were analyzed. The review shows that learning disabilities, developmental delays, alcohol, drug, and substance abuse, were crucial factors that increased the risk of readmission. Greater access to mental health services in residential treatment and improved crisis intervention in congregate care settings were indicated as factors that reduce the risk of readmission. Conclusion: High rates of readmission may adversely impact healthcare spending. This study suggests a need for focused health policies to address readmission factors and improve community-based care.
Physiological Disorder Diagnosis of Plant Leaves Based on Full-Spectrum Hyperspectral Images with Convolutional Neural Network
Authors: Yang Myongkyoon
Published: September 2022

The prediction and early detection of physiological disorders based on the nutritional conditions and stress of plants are extremely vital for the growth and production of crops. High-throughput phenotyping is an effective nondestructive method to understand this, and numerous studies are being conducted with the development of convergence technology. This study analyzes physiological disorders in plant leaves using hyperspectral images and deep learning algorithms. Data on seven classes for various physiological disorders, including normal, prediction, and the appearance of symptom, were obtained for strawberries subjected to artificial treatment. The acquired hyperspectral images were used as input for a convolutional neural network algorithm without spectroscopic preprocessing. To determine the optimal model, several hyperparameter tuning and optimizer selection processes were performed. The Adam optimizer exhibited the best performance with an F1 score of ≥0.95. Moreover, the RMSProp optimizer exhibited slightly similar performance, confirming the potential for performance improvement. Thus, the novel possibility of utilizing hyperspectral images and deep learning algorithms for nondestructive and accurate analysis of the physiological disorders of plants was shown.
Ankle-Brachial Index and Arterial Stiffness, Modulate the Exertional Capacity of High-Frequency Training Athletes
Authors: Pellegrino Raffaello , Sparvieri Eleonora , Di Blasio Andrea , Barassi Giovanni , Murgia Massimiliano , Ripari Patrizio , Di Iorio Angelo
Published: September 2022

Ankle-brachial index and arterial stiffness are associated with leg function in the elderly and in patients with peripheral arterial disease. Little is known about the meaning of these parameters in young and trained subjects and how they are related to physical performance. The main objective was to evaluate the mediating role of arterial stiffness and ankle-brachial index in physical performance. In a cross-sectional, case-control study, 240 male athletes were consecutively enrolled from the Laboratory of Cardiology and Sports Medicine, “G. d’Annunzio” University (Italy). All the subjects underwent the examination protocol for the annual medical evaluation for sport participation. Soccer (football) players compared to runners showed a lower level of ankle-brachial index, higher arterial stiffness, and lower systolic and diastolic blood pressure. In the treadmill stress test, soccer players compared to runners showed a greater maximal aerobic capacity. Differences in cardiovascular performance between soccer players and runners were mediated by better arterial stiffness and low level of ankle-brachial index; the estimated effect was 0.11 ± 0.05 and 0.24 ± 0.06, respectively. Vigorous strength training drops blood pressure and increases arterial stiffness. Taken together, our findings would seem to suggest that ABI and CAVI could be used as markers for athletes’ performance.
Influence of Teaching Styles on the Learning Academic Confidence of Teachers in Training
Authors: Granero-Gallegos Antonio , Escaravajal Juan Carlos , López-García Ginés David , Baños Raúl
Published: September 2022

The objective of this research was to analyze the mediation of academic engagement and the satisfaction of basic needs between teaching styles and academic confidence amongst teachers during initial training. The research design was observational, descriptive, cross-sectional, and non-randomized. In total, 920 university students in initial teacher training participated (61.85% women) (Mage = 24.73; SD = 5.34). The scales of interpersonal teaching styles, the satisfaction of basic psychological needs, academic engagement, and academic confidence were used, and a structural equation analysis with latent variables was carried out. The results highlight the important mediating role played by the satisfaction of basic psychological needs between the autonomous support style and the academic confidence of the trainee teachers. This research also highlights the importance, both for teachers and researchers, of using an autonomous support style, as well as the creation of a context for encouraging the satisfaction of basic psychological needs, to promote academic confidence in initial teacher training programs at university.
Design and Control of an Underwater Robot Based on Hybrid Propulsion of Quadrotor and Bionic Undulating Fin
Authors: Zeng Xiaofeng , Xia Minghai , Luo Zirong , Shang Jianzhong , Xu Yuze , Yin Qian
Published: September 2022

Stable, quiet, and efficient propulsion methods are essential for underwater robots to complete their tasks in a complex marine environment. However, with a single propulsion method, such as propeller propulsion and bionic propulsion, it is difficult to achieve high efficiency and high mobility at the same time. Based on the advantages of the high-efficiency propulsion of a bionic undulating fin and the stable control of the propeller, an underwater robot based on the hybrid propulsion of a quadrotor and undulating fin is proposed in this paper. This paper first introduces the mechanical implementation of the underwater robot. Then, based on kinematic modeling and theoretical derivation, the underwater motion and attitude of the robot are analyzed and the 6-DOF dynamic equation of the robot is established. Finally, the underwater motion performance of the robot is verified through field experiments. The experimental results show that the robot can realize the heave motion, surge motion, and in-situ steering motion independently and can hover stably. When the undulating frequency is 6 Hz, the maximum propulsion speed of the robot can reach up to 1.2 m/s (1.5 BL/s).
Fungi Classification in Various Growth Stages Using Shortwave Infrared (SWIR) Spectroscopy and Machine Learning
Authors: Liu Zhuo , Li Yanjie
Published: September 2022

Dark septate endophytes (DSEs) fungi are beneficial to host plants with regard to abiotic stress. Here, we examined the capability of SWIR spectroscopy to classify fungus types and detected the growth stages of DSEs fungi in a timely, non-destructive and time-saving manner. The SWIR spectral data of five DSEs fungi in six growth stages were collected, and three pre-processing methods and sensitivity analysis (SA) variable selection methods were performed using a machine learning model. The results showed that the De-trending + first Derivative (DET_FST) processing spectra combined with the support vector machine (SVM) model yielded the best classification accuracy for fungi classification at different growth stages and growth stage detection on different fungus types. The mean accuracy of generic model for fungi classification and growth stage detection are 0.92 and 0.99 on the calibration set, respectively. Seven important bands, 1164, 1456, 2081, 2272, 2278, 2448 and 2481 nm, were found to be related to the SVM fungi classification. This study provides a rapid and efficient method for the classification of fungi in different growth stages and the detection of fungi growth stage of various types of fungi and could serve as a tool for fungi study.
Engineered Saccharomyces cerevisiae for the De Novo Biosynthesis of (−)-Menthol
Authors: Lv Xueqin , Zhou Xuan , Ma Jun , Tao Mengrui , Liu Yanfeng , Li Jianghua , Du Guocheng , Liu Long
Published: September 2022

Menthol, a high-value commodity monoterpenoid chemical, holds an important market share commercially because of its distinct functions. The menthol on the market mainly originates from plant extraction, which is facing challenges such as the seasonal fluctuations and long growth cycle of plants. Therefore, this study attempted to realize the de novo synthesis of menthol through microbial fermentation. First, through heterologous expression and subcellular localization observation, a synthetic route from glucose to (−)-menthol was successfully designed and constructed in Saccharomyces cerevisiae. Then, the mevalonate (MVA) pathway was enhanced, and the expression of farnesyl diphosphate synthase (ERG20) was dynamically regulated to improve the synthesis of D-limonene, a key precursor of (−)-menthol. Shake flask fermentation results showed that the D-limonene titer of the recombinant strain reached 459.59 mg/L. Next, the synthesis pathway from D-limonene to (−)-menthol was strengthened, and the fermentation medium was optimized. The (−)-menthol titer of 6.28 mg/L was obtained, implying that the de novo synthesis of menthol was successfully realized for the first time. This study provides a good foundation for the synthesis of menthol through microbial fermentation.
Does FDI Promote the Resource Curse in Nigeria?
Authors: Shobande Olatunji Abdul
Published: September 2022

This study investigated whether Foreign Direct Investment (FDI) supported the resource curse hypothesis in Nigeria. The precise methodological contribution was based on the Vector Error Correction and Granger causality test. The finding showed cointegration among the variables, whereas the speed of adjustment was slightly low. Similarly, natural resource to gross domestic product, FDI, and exchange rate unidirectionally Granger cause economic welfare, whereas bidirectional Granger causality is observed between indicators of natural resources to export, trade, and economic welfare. The results clearly indicate that FDI and natural resource management could improve economic wellbeing, although with a cost of volatility in the exchange rate and utilisation of resources. Thus, the study recommends the urgent need for effective and efficient management of the country’s natural resources to attract foreign direct investment and generate growth that can contribute meaningfully to the welfare of the citizens. Likewise, there is a need to diversify oil resources to other non-natural resources for the economy to stimulate growth and reduce the vulnerability of the economy to external shocks.