bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcription of biochemical defenses by the harmful brown tide pelagophyte, Aureococcus anophagefferens, in response to the protozoan grazer, Oxyrrhis marina
Authors: Dawydiak Walter , Gobler Christopher J.
Published: December 2023

Aureococcus anophagefferens is a small marine pelagophyte that forms recurrent harmful brown tides blooms with adverse ecological and economic impacts. During blooms, A. anophagefferens experiences lower zooplankton grazing mortality than other phytoplankton potentially due to the synthesis of anti-predator compounds including extracellular polysaccharides. This study characterized the transcriptomic response of A. anophagefferens when exposed to the protozooplankton, Oxyrrhis marina, and assessed whether this response involved chemical cues. Transcriptomes were generated from A. anophagefferens populations grown at high (1×106 cells mL−1) and low (5×105 cells mL−1) cell densities incubated directly with O. marina or receiving only filtrate from co-cultures of A. anophagefferens and O. marina to evaluate the role of chemical cues. There were a greater number of genes differentially expressed in response to grazing in the lower concentration of A. anophagefferens compared to the high concentration treatment and in response to direct grazing compared to filtrate. KEGG pathway analysis revealed that direct grazer exposure led to a significant increase in transcripts of genes encoding secondary metabolite production (p < 0.001). There was broad transcriptional evidence indicating the induction of biosynthetic pathways for polyketides and sterols in response to zooplankton grazers, compounds associated with damage to marine organisms. In addition, exposure to O. marina elicited changes in the abundance of transcripts associated with carbohydrate metabolism that could support the formation of an extracellular polysaccharide matrix including genes related to glycoprotein synthesis and carbohydrate transport. Collectively, these findings support the hypothesis that A. anophagefferens can induce biochemical pathways that reduce grazing mortality and support blooms.
Early intervention and adding effective doses of EGb761 like Ginkgo extract slow down dementia progression: insights to the neurovascular unit
Authors: Özge Aynur , Ghouri Reza , Öksüz Nevra , Taşdelen Bahar
Published: December 2023

Background

Dementia is a progressive neurodegenerative disorder characterized by cognitive decline, memory impairment, and functional deterioration. Pharmacological interventions play a crucial role in managing dementia symptoms and potentially slowing down disease progression.

Objectives

This study aimed to investigate the impact of pharmacological interventions, including acetylcholinesterase inhibitors (AChEIs), memantine, and Gingko extract, on the progression of dementia, with a specific focus on mild cognitive impairment (MCI), Alzheimer’s disease (AD), and non-Alzheimer dementias.

Methods

A total of 547 participants out of 3,547 cases in a specific dataset followed by the same author, including healthy controls, individuals with MCI, AD, and non-Alzheimer dementias, were included in this study. The follow-up duration was up to 211 months, allowing for a minimum 3 visits comprehensive assessment of disease progression. The treatment approaches included AChEIs, memantine, and combination therapy, with variations in the starting time for these treatments based on the dementia type.

Results

The use of AChEIs and memantine showed efficacy in improving cognitive function and overall function in individuals with MCI, AD, and non-AD dementias. Combination therapy EGb761 like Gingko extract with AChEIs and/or Memantine demonstrated a slower progression compared to AChEIs alone in individuals with prodromal dementia (MCI) and AD. The starting time for memantine and combination therapy was earlier in non-AD dementia cases compared to AD dementia cases and prodromal dementia.

Conclusion

Pharmacological interventions, particularly the use of AChEIs and memantine, can have a positive impact on cognitive function and overall function in individuals with dementia. The combination of AChEIs with EGb761 like Gingko extract may provide additional benefits in slowing down disease progression in AD cases. Early recognition and accurate classification of MCI subtypes are crucial, and the use of EGb761 like Gingko extract is recommended for symptomatic treatment. Future personalized risk predictions based on biomarker constellations may further enhance the multi-target treatment approaches of MCI and different dementia types.
Botulinum neurotoxin type A in the interdisciplinary treatment of sialorrhea in adults and children—update and practice recommendations
Authors: Jost Wolfgang H. , Bäumer Tobias , Bevot Andrea , Birkmann Ulrich , Buhmann Carsten , Grosheva Maria , Guntinas-Lichius Orlando , Laskawi Rainer , Paus Sebastian , Pflug Christina , Schroeder A. Sebastian , Spittau Björn , Steffen Armin , Wilken Bernd , Winterholler Martin , Berweck Steffen
Published: December 2023

Sialorrhea is defined as a chronic excessive flow of saliva from the mouth, often with adverse consequences for health and quality of life of patients. In addition to currently used non-drug treatment and systemic drugs, intraglandular Botulinum Neurotoxin A (BoNT/A) injections have been examined in case studies, controlled trials and clinical practice. Two pivotal Phase III trials recently led to market approval in the USA and EU for IncobotulinumtoxinA [Xeomin®, IncoBoNT/A, Clostridium botulinum neurotoxin type A (150 kD), free from complexing proteins, Merz Pharmaceuticals GmbH] for treatment of chronic sialorrhea in adults and pediatric patients. This review provides a multidisciplinary approach to discuss the current state of sialorrhea therapy as well as benefits and current limitations of BoNT/A injections. A consensus regarding treatment recommendations made available to physicians in Germany in 2022 has now been updated here for presentation to an international audience. This review provides a framework including a flow chart for patient selection, recommendations for dosing and the injection process, as well as a discussion of therapeutic goals, long-term benefits and safety aspects. This review is aimed at supporting physicians in developing multidisciplinary and individualized treatment approaches to achieve optimal benefits for patients.
Materials qualification through the Nuclear Science User Facilities (NSUF): a case study on irradiated PM-HIP structural alloys
Authors: Wharry Janelle P. , Guillen Donna Post , Clement Caleb D. , Bin Habib Saquib , Jiang Wen , Zhao Yangyang , Lu Yu , Wu Yaqiao , Shiau Ching-Heng , Frazer David , Heidrich Brenden J. , Knight Collin , Gandy David W.
Published: December 2023

This article presents neutron irradiation and post-irradiation examination (PIE) capabilities available to the nuclear materials research community through the US Department of Energy’s Nuclear Science User Facilities (NSUF). The pressing need to deploy advanced nuclear reactors to combat climate change requires qualification of new fuels and materials. Among advanced manufacturing processes, powder metallurgy with hot isostatic pressing (PM-HIP) is nearest to becoming qualified for nuclear applications. This article provides examples from a recent irradiation and PIE program on a series of structural alloys fabricated by PM-HIP to illustrate how NSUF capabilities can be used to generate qualification data. The neutron irradiation experiments are described, and a sampling of results from tensile testing, nanoindentation, transmission electron microscopy, and atom probe tomography are presented, showing the favorable performance of PM-HIP alloys compared to their cast or forged counterparts under irradiation. This article provides a perspective on leveraging NSUF for future nuclear fuels and materials testing and qualification.
Are postprandial glucose responses sufficiently person-specific to use in personalized dietary advice? Design of the RepEAT study: a fully controlled dietary intervention to determine the variation in glucose responses
Authors: Daanje Monique , Siebelink Els , Vrieling Frank , van den Belt Maartje , van der Haar Sandra , Gerdessen Johanna C. , Kersten Sander , Esser Diederik , Afman Lydia A.
Published: December 2023

Introduction

An elevated postprandial glucose response is associated with an increased risk of cardiometabolic diseases. Existing research suggests large heterogeneity in the postprandial glucose responses to identical meals and food products between individuals, but the effect of other consumed meals during the day and the order of meals during the day on the heterogeneity in postprandial glucose responses still needs to be investigated. In addition, the robustness of the glucose responses to meals or foods is still unknown.

Objectives

The overall aim of the project is to assess whether the glucose response to a meal is sufficiently person-specific to use in personalized dietary advice. We aim to answer the question: “How replicable are glucose responses to meals within individuals and how consistent is the variation in glucose responses between individuals?”

Methods

The question will be assessed under standardized conditions of a 9-week fully controlled dietary intervention in which all meals are the same between individuals and consumed in a fixed order at a fixed time. 63 apparently healthy men and women with a BMI of 25–40 kg/m2and aged 45–75 years were enrolled in the RepEAT study (NCT05456815), of whom 53 participants completed the study. The RepEAT study comprised a fully controlled dietary intervention of nine weeks, consisting of three repetitive periods of three weeks. Within each three-week period, a variety of meals and food products were offered during breakfast, lunch, dinner and in between meal snacks. Throughout the dietary intervention, glucose was continuously monitored using Freestyle Libre Pro IQ monitors. Physical activity was monitored using the ActiGraph and ActivPAL. To measure the association between glucose responses and an individual’s phenotype, various measurements were performed before the start of the dietary intervention including an oral glucose tolerance test, a high-fat mixed meal challenge, assessment of body fat distribution including liver fat (MRI/MRS), and cardiometabolic markers.

Discussion

The repetitive and fully controlled nature of the dietary study allows detailed assessment of the replicability of the glucose responses to meals and food products within individuals. Furthermore, the consistency of the variation between individuals independent of insulin resistance will be determined.
Unraveling the safety of adjuvant radiotherapy in prostate cancer: impact of older age and hypofractionated regimens on acute and late toxicity - a multicenter comprehensive analysis
Authors: Buwenge Milly , Macchia Gabriella , Cavallini Letizia , Cortesi Annalisa , Malizia Claudio , Bianchi Lorenzo , Ntreta Maria , Arcelli Alessandra , Capocaccia Ilaria , Natoli Elena , Cilla Savino , Cellini Francesco , Tagliaferri Luca , Strigari Lidia , Cammelli Silvia , Schiavina Riccardo , Brunocilla Eugenio , Morganti Alessio Giuseppe , Deodato Francesco
Published: December 2023

Background

The objective of this study was to assess the impact of age and other patient and treatment characteristics on toxicity in prostate cancer patients receiving adjuvant radiotherapy (RT).

Materials and methods

This observational study (ICAROS-1) evaluated both acute (RTOG) and late (RTOG/EORTC) toxicity. Patient- (age; Charlson’s comorbidity index) and treatment-related characteristics (nodal irradiation; previous TURP; use, type, and duration of ADT, RT fractionation and technique, image-guidance systems, EQD2 delivered to the prostate bed and pelvic nodes) were recorded and analyzed.

Results

A total of 381 patients were enrolled. The median EQD2 to the prostate bed (α/β=1.5) was 71.4 Gy. The majority of patients (75.4%) were treated with intensity-modulated radiation therapy (IMRT) or volumetric-modulated arc therapy (VMAT). Acute G3 gastrointestinal (GI) and genitourinary (GU) toxicity rates were 0.5% and 1.3%, respectively. No patients experienced >G3 acute toxicity. The multivariable analysis of acute toxicity (binomial logistic regression) showed a statistically significant association between older age (> 65) and decreased odds of G≥2 GI acute toxicity (OR: 0.569; 95%CI: 0.329-0.973; p: 0.040) and decreased odds of G≥2 GU acute toxicity (OR: 0.956; 95%CI: 0.918-0.996; p: 0.031). The 5-year late toxicity-free survival rates for G≥3 GI and GU toxicity were 98.1% and 94.5%, respectively. The only significant correlation found (Cox’s regression model) was a reduced risk of late GI toxicity in patients undergoing hypofractionation (HR: 0.38; 95% CI: 0.18-0.78; p: 0.008).

Conclusions

The unexpected results of this analysis could be explained by a “response shift bias” concerning the protective effect of older age and by treatment in later periods (using IMRT/VMAT) concerning the favorable effect of hypofractionation. However, overall, the study suggests that age should not be a reason to avoid adjuvant RT and that the latter is well-tolerated even with moderately hypofractionated regimens.
A hematological parameter-based model for distinguishing non-puerperal mastitis from invasive ductal carcinoma
Authors: Wang Zhichun , Hua Lin , Liu Xiaofeng , Chen Xueli , Xue Guohui
Published: December 2023

Purpose

Non-puerperal mastitis (NPM) accounts for approximately 4-5% of all benign breast lesions. Ultrasound is the preferred method for screening breast diseases; however, similarities in imaging results can make it challenging to distinguish NPM from invasive ductal carcinoma (IDC). Our objective was to identify convenient and objective hematological markers to distinguish NPM from IDC.

Methods

We recruited 89 patients with NPM, 88 with IDC, and 86 with fibroadenoma (FA), and compared their laboratory data at the time of admission. LASSO regression, univariate logistic regression, and multivariate logistic regression were used to screen the parameters for construction of diagnostic models. Receiver operating characteristic curves, calibration curves, and decision curves were constructed to evaluate the accuracy of this model.

Results

We found significant differences in routine laboratory data between patients with NPM and IDC, and these indicators were candidate biomarkers for distinguishing between the two diseases. Additionally, we evaluated the ability of some classic hematological markers reported in previous studies to differentiate between NPM and IDC, and the results showed that these indicators are not ideal biomarkers. Furthermore, through rigorous LASSO and logistic regression, we selected age, white blood cell count, and thrombin time to construct a differential diagnostic model that exhibited a high level of discrimination, with an area under the curve of 0.912 in the training set and with 0.851 in the validation set. Furthermore, using the same selection method, we constructed a differential diagnostic model for NPM and FA, which also demonstrated good performance with an area under the curve of 0.862 in the training set and with 0.854 in the validation set. Both of these two models achieved AUCs higher than the AUCs of models built using machine learning methods such as random forest, decision tree, and SVM in both the training and validation sets.

Conclusion

Certain laboratory parameters on admission differed significantly between the NPM and IDC groups, and the constructed model was designated as a differential diagnostic marker. Our analysis showed that it has acceptable efficiency in distinguishing NPM from IDC and may be employed as an auxiliary diagnostic tool.
Impact of intrafraction motion in pancreatic cancer treatments with MR-guided adaptive radiation therapy
Authors: Rusu Doris N. , Cunningham Justine M. , Arch Jacob V. , Chetty Indrin J. , Parikh Parag J. , Dolan Jennifer L.
Published: December 2023

Purpose

The total time of radiation treatment delivery for pancreatic cancer patients with daily online adaptive radiation therapy (ART) on an MR-Linac can range from 50 to 90 min. During this period, the target and normal tissues undergo changes due to respiration and physiologic organ motion. We evaluated the dosimetric impact of the intrafraction physiological organ changes.

Methods

Ten locally advanced pancreatic cancer patients were treated with 50 Gy in five fractions with intensity-modulated respiratory-gated radiation therapy on a 0.35-T MR-Linac. Patients received both pre- and post-treatment volumetric MRIs for each fraction. Gastrointestinal organs at risk (GI-OARs) were delineated on the pre-treatment MRI during the online ART process and retrospectively on the post-treatment MRI. The treated dose distribution for each adaptive plan was assessed on the post-treatment anatomy. Prescribed dose volume histogram metrics for the scheduled plan on the pre-treatment anatomy, the adapted plan on the pre-treatment anatomy, and the adapted plan on post-treatment anatomy were compared to the OAR-defined criteria for adaptation: the volume of the GI-OAR receiving greater than 33 Gy (V33Gy) should be ≤1 cubic centimeter.

Results

Across the 50 adapted plans for the 10 patients studied, 70% were adapted to meet the duodenum constraint, 74% for the stomach, 12% for the colon, and 48% for the small bowel. Owing to intrafraction organ motion, at the time of post-treatment imaging, the adaptive criteria were exceeded for the duodenum in 62% of fractions, the stomach in 36%, the colon in 10%, and the small bowel in 48%. Compared to the scheduled plan, the post-treatment plans showed a decrease in the V33Gy, demonstrating the benefit of plan adaptation for 66% of the fractions for the duodenum, 95% for the stomach, 100% for the colon, and 79% for the small bowel.

Conclusion

Post-treatment images demonstrated that over the course of the adaptive plan generation and delivery, the GI-OARs moved from their isotoxic low-dose region and nearer to the dose-escalated high-dose region, exceeding dose-volume constraints. Intrafraction motion can have a significant dosimetric impact; therefore, measures to mitigate this motion are needed. Despite consistent intrafraction motion, plan adaptation still provides a dosimetric benefit.
Assessment and indicators of hallux limitus related with quality of life and foot health in school children
Authors: Cuevas-Martínez Claudia , Becerro-de-Bengoa-Vallejo Ricardo , Losa-Iglesias Marta Elena , Navarro-Flores Emmanuel , Pérez-Palma Laura , Martiniano João , López-López Daniel , Casado-Hernández Israel , Gómez-Salgado Juan
Published: December 2023

Background

Functional Hallux Limitus (FHL) is a dynamic foot dysfunction characterized by a limitation of hallux dorsiflexion when the first metatarsal head is under load. FHL plays a role in the development of osteoarthrosis in the first metatarsophalangeal joint (IMTPJ). Forefoot disorders can significantly impact an individual's quality of life, leading to dysfunction and pain. The aim of this project was to evaluate the quality of life of school-aged individuals with and without FHL using the Foot Health Status Questionnaire (FHSQ).

Methods

A case-control study was conducted to evaluate the outcomes in paediatric age. A total sample of 116 children between 6 and 12 years old was used to conduct this research. The sample was divided into two groups: (i) the healthy group (n = 58) and the FHL group (n = 58). The FHSQ was completed and the FHL test was performed in a seated position to classify the patients into the selected group.

Results

Non-significant changes were observed when the mean values of the FHSQ domains were compared between the groups with and without FHL, except for the “general foot health” domain (p = 0,024) associated with the specific foot health section (section 1) of the Questionnaire. For the domains linked with the general well-being section (section 2), there was not a statistically difference in the mean of the scores obtained between the two school-aged groups with and without FHL, being slightly lower in the group with the presence of FHL for the overall health and physical function domains. Both the healthy and case groups obtained and identical range of scores (10–100) for the “foot pain” domain. Nevertheless, the mean of the score was lower for the participants with FHL.

Conclusions

The perception of the quality of general foot health was poorer in the school-aged group with FHL. Variables such as foot pain and footwear are likely contributors influencing the perception of foot health quality. The school-aged population with FHL faces a decline in the quality of foot life. Ensuring adequate foot control in children and implementing future foot programs for this population are imperative for enhancing school children's perception of foot health and managing the development of pain and footwear-related issues.
Tumor necrosis factor receptor-associated cycle syndrome: a case report and literature review
Authors: Li Ziwei , Jing Xiaoping , Zhang Shuya , Liu Tiantian , Guo Qingyin
Published: December 2023

Recurring episodes of fever characterize tumor necrosis factor receptor-associated periodic syndrome (TRAPS) which is autosomal dominant. The primary symptoms of patients with TRAPS include prolonged fever, abdominal pain, muscle pain, and skin rashes. The prevalence of TRAPS appeared higher in Western countries than in Asian countries. Herein, we present the case of a 13-year-old girl who experienced intermittent fever for 8 years, with episodes that occur every 2 years. The patient demonstrated periodic fever, headache, vomiting, rash, and elevated inflammatory marker levels during the disease course. A heterozygous C55Y mutation was identified via a direct DNA sequencing of her genomic DNA. This mutation is located in exon 4 of TNFRSF1A. Genetic studies of her sister and mother revealed that they possessed the C55Y heterozygous mutation without demonstrating any clinical signs, while the father did not. Further, we conducted a thorough assessment of the literature and compiled the information from the eight TRAPS case series.
Reliability of a semi-tethered front crawl sprint performance test in adolescent swimmers
Authors: Szczepan Stefan , Wróblewska Zofia , Klich Sebastian , Michalik Kamil , Gonjo Tomohiro , Olstad Bjørn Harald , Rejman Marek
Published: December 2023

This study aimed to evaluate the test-retest reliability of a sprint performance test with semi-tethered front crawl swimming to indirectly assess the current potential to perform at maximal anaerobic effort in adolescent swimmers. Eight adolescent swimmers participated in this study (gender: females (n = 4) aged 13.0 ± 0.8 years, body height 1.6 ± 0.0 m, body mass 50.1 ± 4.5 kg; and males (n = 4) aged 13.3 ± 1.3 years, body height 1.7 ± 0.1 m, body mass 59.0 ± 8.2 kg. The testing protocol consisted of two trials of 25 m semi-tethered front crawl swimming with maximal effort and with 1 kg resisted isotonic load. Velocity data were recorded automatically by the 1080 Sprint device for 15 m (between 3 m and 18 m). The Fast Fourier Transform algorithm filtered raw instantaneous swimming velocity data in distance (time) function. A third-degree polynomial was used to extract the individual velocity profile, from which the following variables were chosen for test-retest reliability and the assessment of sprint performance: ttrial15, vmax, vmin, tvto max, tvat max, Dto vmax, Dat vmax, fatigue index. Parameters such as vmax, vmin, and ttrial15 were estimated from swimming velocity profiles and considered as reliable. The CV showed low variance <5%; while ICC2,1 demonstrated respectively good (ICC2,1: 0.88), very good (ICC2,1: 0.95), and excellent (ICC2,1: 0.98) rate of relative reliability; and the Bland-Altman index revealed an acceptable agreement (LoA ≤5%) between two measurements. The sprint performance test based on semi-tethered front crawl swimming confirmed that ttrial15, vmax, and vmin were reliable variables to indirectly indicate a potential to perform the maximal anaerobic effort among adolescent swimmers. The evaluation of the swimming velocity profiles allows coaches to monitor the adaptive changes of performance during the training process.
Zero-derivation in Korean: the effect of covert structure in real-time processing
Authors: Kim Nayoun , Li Ziying , Byeon Seonghyeon , Lee Chaejin
Published: December 2023

Korean words likebalgda‘bright/become bright’ andgilda‘long/become long’ are categorially ambiguous; they can appear as both adjectives and verbs. Some suggest that these words are listed under separate lexical entries, while others propose that they share one single lexical entry, and that the verb form is morphologically derived from the base adjective through a process called zero derivation. This study presents the results of a real-time experiment that investigates whether these words involve zero derivation and if so, how zero derivation may affect the real-time processing of these words. Our findings suggest that the reader recognizes the base adjective and obtains the derived-verb form by virtue of adding a covert category-changing morpheme in real-time sentence processing. This study provides promising evidence of the zero derivation of Korean categorially ambiguous adjectives and verbs, as well as crosslinguistic evidence of the role of covert structure in lexical access.
Sustainable planning in Wuhan City during COVID-19: an analysis of influential factors, risk profiles, and clustered patterns
Authors: Zhou Peng , Zhang Hailu , Liu Lanjun , Pan Yue , Liu Yating , Sang Xuanhao , Liu Chaoqun , Chen Zixuan
Published: December 2023

The outbreak of novel coronavirus pneumonia (COVID-19) is closely related to the intra-urban environment. It is important to understand the influence mechanism and risk characteristics of urban environment on infectious diseases from the perspective of urban environment composition. In this study, we used python to collect Sina Weibo help data as well as urban multivariate big data, and The random forest model was used to measure the contribution of each influential factor within to the COVID-19 outbreak. A comprehensive risk evaluation system from the perspective of urban environment was constructed, and the entropy weighting method was used to produce the weights of various types of risks, generate the specific values of the four types of risks, and obtain the four levels of comprehensive risk zones through the K-MEANS clustering of Wuhan’s central urban area for zoning planning. Based on the results, we found: ①the five most significant indicators contributing to the risk of the Wuhan COVID-19 outbreak were Road Network Density, Shopping Mall Density, Public Transport Density, Educational Facility Density, Bank Density. Floor Area Ration, Poi Functional Mix ②After streamlining five indicators such as Proportion of Aged Population, Tertiary Hospital Density, Open Space Density, Night-time Light Intensity, Number of Beds Available in Designated Hospitals, the prediction accuracy of the random forest model was the highest. ③The spatial characteristics of the four categories of new crown epidemic risk, namely transmission risk, exposure risk, susceptibility risk and Risk of Scarcity of Medical Resources, were highly differentiated, and a four-level integrated risk zone was obtained by K-MEANS clustering. Its distribution pattern was in the form of “multicenter-periphery” gradient diffusion. For the risk composition of the four-level comprehensive zones combined with the internal characteristics of the urban environment in specific zones to develop differentiated control strategies. Targeted policies were then devised for each partition, offering a practical advantage over singular COVID-19 impact factor analyses. This methodology, beneficial for future public health crises, enables the swift identification of unique risk profiles in different partitions, streamlining the formulation of precise policies. The overarching goal is to maintain regular social development, harmonizing preventive measures and economic efforts.
A control banding method for chemical risk assessment in occupational settings in France
Authors: Aachimi Abir , Marc Florian , Bonvallot Nathalie , Clerc Frederic
Published: December 2023

Background

This study describes a method whose aim is to help companies assess the chemical occupational risks related to labeled products and industrial chemical emissions. The method is intended to be used by industrial hygienists at the scale of one company. Both inhalation and cutaneous exposure routes are taken into account.

Methods

The method relies on a control-banding scheme. A work situation is described by exposure parameters such as the process or the local exhaust ventilation and by the hazard of the product. Each possible value of the parameters is associated with a “band,” which is associated with an integer value. The multiplication of these values results in a score, which represents a priority for intervention. The higher the score, the more the situation warrants investigation for implementing prevention measures, such as chemical substitution and the addition of local exhaust ventilation. To simplify communication, the priority is associated with a colored priority band: red for “very high priority,” orange for “high priority,” and green for “moderate priority.” The priority bands are computed for all work situations performed in a company.

Results

An example of the use of this method is described in a French façade insulation company.

Conclusion

A tool named Seirich was developed to implement this method and promote good practices for helping industrial hygienists in the prioritization of interventions for reducing chemical risk in France.
Comparison of healthcare workers and non-healthcare workers in terms of obsessive-compulsive and depressive symptoms during COVID-19 pandemic: a longitudinal case-controlled study
Authors: Uyar Betul , Donmezdil Suleyman
Published: December 2023

Objective

The aim of this study was to investigate the obsessive-compulsive and depressive symptoms of healthcare workers in a case-control setting as longitudinal.

Method

In this study included 49 healthcare workers and 47 non-health workers. A sociodemographic data form, the Maudsley Obsessive-Compulsive Inventory (MOCI), the Symptom Checklist-90 (SCL-90), and the Hamilton Depression Rating Scale (HAM-D) were used to assess individuals between June 1, 2020 and June 30, 2021. We assessed the same healthcare workers after 12 months on June 30, 2021 using MOCI, HAM-D, and SCL-90.

Results

MOCI and SCL-90 obsessive-compulsive subscale scores were significantly higher in the healthcare workers than in the non-health workers. When we assessed MOCI, HAM-D, and SCL-90 obsessive-compulsive subscale scores after 12 months, there was a statistically significant decrease in the scores of all three scales among the healthcare workers.

Conclusion

The results of the study showed that healthcare workers were more likely to have obsessive-compulsive symptoms than non-health workers in the early part of the pandemic on June 1, 2020, as shown by their scores on MOCI and the obsessive-compulsive subscale of SCL-90. When we assessed the same participants after 12 months (June 30, 2021), both MOCI and SCL-90 obsessive-compulsive subscale scores had decreased significantly. In contrast to these results, HAM-D scores significantly increased.
The Causes and Forecasting of Icing Events on Power Transmission Lines in Southern China: A Review and Perspective
Authors: Wang Luyao , Chen Zechang , Zhang Wenjie , Lu Zhumao , Cheng Yang , Qu Xiaoli , Gul Chaman , Yang Yuanjian
Published: December 2023

The icing on power transmission lines, as a major hazard affecting the safety of electricity usage in China during winter, poses a significant challenge in systematically evaluating the weather conditions and their distribution characteristics during the icing period. Understanding the interaction between the microterrain and micrometeorology and achieving a refined analysis of the physical mechanisms during the icing process remain difficult tasks in this field. These are crucial aspects for the development of more accurate icing prediction models across southern China. Therefore, this study provides a comprehensive review and summary of the current research state and progress in the study of power transmission line icing in southern China from three perspectives: (1) large-scale circulation characteristics; (2) microphysical process, terrain–atmosphere interaction, microtopography and local micrometeorological conditions for the occurrence of icing events; and (3) numerical icing event modeling and forecasting. This study also looks ahead to the scientific issues and technological bottlenecks that need to be overcome for the prediction of ice coating on power transmission lines in southern China. The goal is to provide guidance for the causal analysis and forecasting warnings of power transmission line icing in the complex microterrain of the southern region.
Research on Missing Value Imputation to Improve the Validity of Air Quality Data Evaluation on the Qinghai-Tibetan Plateau
Authors: Wang Yumeng , Liu Ke , He Yuejun , Fu Qiming , Luo Wei , Li Wentao , Liu Xuan , Wang Pengfei , Xiao Siyuan
Published: December 2023

In the Qinghai-Tibet Plateau region, operational deficiencies and limited maintenance capacities often impair automatic air quality monitoring stations. This results in frequent data omissions, compromising the reliability of environmental assessment data. Therefore, an effective data imputation method is required to address the gaps in observational records. Utilizing a Sequence-to-Sequence framework, we introduce a model termed Bidirectional Recurrent Imputation for Time Series-Attention-based Long Short-Term Memory (BRITS-ALSTM). The encoder of BRITS-ALSTM applies BRITS to integrate single-station historical characteristics with multi-station correlation features. Concurrently, the decoder employs LSTM within an attention mechanism to capitalize on previously observed data, thereby generating hourly imputations for missing air quality data values. The model was trained using six types of air quality data from 16 stations across Qinghai Province. Through localized testing and parameter optimization, BRITS-ALSTM achieved a reduction in mean relative error (MRE) by 74.88% compared to the baseline mean-filling approach. Additionally, ablation studies demonstrated an improvement in the coefficient of determination R-squared (R2) from 0.67 to 0.76, outperforming the standalone BRITS. Consequently, BRITS-ALSTM enhances the accuracy of air quality data evaluations in the Tibetan Plateau and offers an efficacious strategy for data imputation in elevated terrains.
Text Classification Based on the Heterogeneous Graph Considering the Relationships between Documents
Authors: Nakajima Hiromu , Sasaki Minoru
Published: December 2023

Text classification is the task of estimating the genre of a document based on information such as word co-occurrence and frequency of occurrence. Text classification has been studied by various approaches. In this study, we focused on text classification using graph structure data. Conventional graph-based methods express relationships between words and relationships between words and documents as weights between nodes. Then, a graph neural network is used for learning. However, there is a problem that conventional methods are not able to represent the relationship between documents on the graph. In this paper, we propose a graph structure that considers the relationships between documents. In the proposed method, the cosine similarity of document vectors is set as weights between document nodes. This completes a graph that considers the relationship between documents. The graph is then input into a graph convolutional neural network for training. Therefore, the aim of this study is to improve the text classification performance of conventional methods by using this graph that considers the relationships between document nodes. In this study, we conducted evaluation experiments using five different corpora of English documents. The results showed that the proposed method outperformed the performance of the conventional method by up to 1.19%, indicating that the use of relationships between documents is effective. In addition, the proposed method was shown to be particularly effective in classifying long documents.
The “StemDif Sensor Test”: A Straightforward, Non-Invasive Assay to Characterize the Secreted Stemness and/or Differentiation Activities of Tumor-Derived Cancer Cell Lines
Authors: Abou Hammoud Aya , Giraud Julie , Gauthereau Xavier , Blanchard Camille , Daburon Sophie , Zese Marco , Molina-Castro Silvia , Dubus Pierre , Varon Christine , Boeuf Helene
Published: December 2023

Cancer stem cells are a subpopulation of tumor cells characterized by their ability to self-renew, induce tumors upon engraftment in animals and exhibit strong resistance to chemotherapy and radiotherapy. These cells exhibit numerous characteristics in common with embryonic stem cells, expressing some of their markers, typically absent in non-pathological adult differentiated cells. The aim of this study was to investigate the potential of conditioned media from cancer stem cells to modulate the fate of Leukemia Inhibitory Factor (LIF)-dependent murine embryonic stem cells (mESCs) as a way to obtain a direct readout of the secretome of cancer cells. A functional assay, “the StemDif sensor test”, was developed with two types of cancer stem cells derived from grade IV glioblastoma (adult and pediatric) or from gastric adenocarcinoma. We show that conditioned media from the selection of adult but not pediatric Glioma-Inducing Cells (GICs) maintain mESCs’ pluripotency in correlation with LIF secretion and activation of STAT3 protein. In contrast, conditioned media from gastric adenocarcinoma cells display LIF-independent stemness and differentiation activities on mESC. Our test stands out for its user-friendly procedures, affordability and straightforward output, positioning it as a pioneering tool for in-depth exploration of cancer stem cell secretome characteristics.
Metabolomics, Lipidomics, and Antipsychotics: A Systematic Review
Authors: Burghardt Kyle J. , Kajy Megan , Ward Kristen M. , Burghardt Paul R.
Published: December 2023

Antipsychotics are an important pharmacotherapy option for the treatment of many mental illnesses. Unfortunately, selecting antipsychotics is often a trial-and-error process due to a lack of understanding as to which medications an individual patient will find most effective and best tolerated. Metabolomics, or the study of small molecules in a biosample, is an increasingly used omics platform that has the potential to identify biomarkers for medication efficacy and toxicity. This systematic review was conducted to identify metabolites and metabolomic pathways associated with antipsychotic use in humans. Ultimately, 42 studies were identified for inclusion in this review, with all but three studies being performed in blood sources such as plasma or serum. A total of 14 metabolite classes and 12 lipid classes were assessed across studies. Although the studies were highly heterogeneous in approach and mixed in their findings, increases in phosphatidylcholines, decreases in carboxylic acids, and decreases in acylcarnitines were most consistently noted as perturbed in patients exposed to antipsychotics. Furthermore, for the targeted metabolomic and lipidomic studies, seven metabolites and three lipid species had findings that were replicated. The most consistent finding for targeted studies was an identification of a decrease in aspartate with antipsychotic treatment. Studies varied in depth of detail provided for their study participants and in study design. For example, in some cases, there was a lack of detail on specific antipsychotics used or concomitant medications, and the depth of detail on sample handling and analysis varied widely. The conclusions here demonstrate that there is a large foundation of metabolomic work with antipsychotics that requires more complete reporting so that an objective synthesis such as a meta-analysis can take place. This will then allow for validation and clinical application of the most robust findings to move the field forward. Future studies should be carefully controlled to take advantage of the sensitivity of metabolomics while limiting potential confounders that may result from participant heterogeneity and varied analysis approaches.
Constitutive HO-1 and CD55 (DAF) Expression and Regulatory Interaction in Cultured Podocytes
Authors: Lianos Elias A. , Wilson Kelsey , Goudevenou Katerina , Detsika Maria G. , Sharma Mukut
Published: December 2023

Overexpression of the inducible heme oxygenase (HO-1) isoform in visceral renal glomerular epithelial cells (podocytes) using in vivo transgenesis methods was shown to increase glomerular expression of the complement regulatory protein decay-accelerating factor (DAF, CD55) and reduce complement activation/deposition in a rat model of immune-mediated injury. In this preliminary study, we assessed whether constitutively expressed HO-1 regulates CD55 expression in cultured rat podocytes. We employed methods of flow cytometry, quantitative (q) RT-qPCR and post-transcriptional HO-1 gene silencing (HO-1 interfering RNA, RNAi), to assess changes in constitutive (basal) levels of podocyte HO-1 and CD55 mRNA in cultured rat podocytes. Additionally, the effect of the HO-1 inducer, heme, on HO-1 and CD55 expression was assessed. Results indicate that rat podocytes constitutively express HO-1 and DAF and that the HO-1 inducer, heme, increases both HO-1 and DAF expression. HO-1 gene silencing using RNA interference (RNAi) is feasible but the effect on constitutive CD55 transcription is inconsistent. These observations are relevant to conditions of podocyte exposure to heme that can activate the complementary cascade, as may occur in systemic or intraglomerular hemolysis.
Vinorelbine Alters lncRNA Expression in Association with EGFR Mutational Status and Potentiates Tumor Progression Depending on NSCLC Cell Lines’ Genetic Profile
Authors: Alsharoh Hasan , Chiroi Paul , Nutu Andreea , Raduly Lajos , Zanoaga Oana , Berindan-Neagoe Ioana
Published: December 2023

Lung cancer remains the leading cause of cancer-related mortality worldwide, with non-small cell lung cancer (NSCLC) as the most common type. In addition, NSCLC has a high mortality rate and an overall adverse patient outcome. Although significant improvements have been made in therapeutic options, effectiveness is still limited in late stages, so the need for a better understanding of the genomics events underlying the current therapies is crucial to aid future drug development. Vinorelbine (VRB) is an anti-mitotic chemotherapy drug (third-generation vinca alkaloid) used to treat several malignancies, including NSCLC. However, despite its widespread clinical use, very little is known about VRB-associated genomic alterations in different subtypes of NSCLC. This article is an in vitro investigation of the cytotoxic effects of VRB on three different types of NSCLC cell lines, A549, Calu-6, and H1792, with a closer focus on post-treatment genetic alterations. Based on the obtained results, VRB cytotoxicity produces modifications on a cellular level, altering biological processes such as apoptosis, autophagy, cellular motility, cellular adhesion, and cell cycle, but also at a genomic level, dysregulating the expression of some coding genes, such as EGFR, and long non-coding RNAs (lncRNAs), including CCAT1, CCAT2, GAS5, MALAT1, NEAT1, NORAD, XIST, and HOTAIR, that are implicated in the mitogen-activated protein kinase (MAPK) signaling pathway. Therefore, although extensive validation is required, these results pave the way towards a better understanding of the cellular and genomic alterations underlying the cytotoxicity of VRB.
Splice-Switching Antisense Oligonucleotides Targeting Extra- and Intracellular Domains of Epidermal Growth Factor Receptor in Cancer Cells
Authors: Balachandran Akilandeswari Ashwini , Raguraman Prithi , Rahimizadeh Kamal , Veedu Rakesh N.
Published: December 2023

Cancer is one of the leading causes of death globally. Epidermal growth factor receptor is one of the proteins involved in cancer cell proliferation, differentiation, and invasion. Antisense oligonucleotides are chemical nucleic acids that bind to target messenger ribonucleic acid and modulate its expression. Herein, we demonstrate the efficacy of splice-modulating antisense oligonucleotides to target specific exons in the extracellular (exon 3) and intracellular (exon 18, 21) domains of epidermal growth factor receptor. These antisense oligonucleotides were synthesized as 25mer 2′-O methyl phosphorothioate-modified ribonucleic acids that bind to complementary specific regions in respective exons. We found that PNAT524, PNAT525, PNAT576, and PNAT578 effectively skipped exon 3, exon 18, and exon 21 in glioblastoma, liver cancer, and breast cancer cell lines. PNAT578 treatment also skipped partial exon 19, complete exon 20, and partial exon 21 in addition to complete exon 21 skipping. We also found that a cocktail of PNAT576 and PNAT578 antisense oligonucleotides performed better than their individual counterparts. The migration potential of glioblastoma cancer cells was reduced to a greater extent after treatment with these antisense oligonucleotides. We firmly believe that using these splice-modulating antisense oligonucleotides in combination with existing EGFR-targeted therapies could improve therapeutic outcomes.
p38 Mitogen-Activated Protein Kinase Inhibition of Mesenchymal Transdifferentiated Tumor Cells in Head and Neck Squamous Cell Carcinoma
Authors: Federspiel Julia , Greier Maria do Carmo , Ladányi Andrea , Dudas Jozsef
Published: December 2023

High mortality in head and neck squamous cell carcinoma (HNSCC) is due to recurrence, metastasis, and radiochemotherapy (RCT) resistance. These phenomena are related to the tumor cell subpopulation undergoing partial epithelial to mesenchymal transition (pEMT). Repeated transforming growth factor-beta (TGF-beta-1) treatment via the p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway induces pEMT in SCC-25 HNSCC cells, and activates and stabilizes the pro-EMT transcription factor Slug. We investigated the growth inhibitory, cisplatin-sensitizing, and pro-apoptotic effects of p38 MAPK inhibition in cisplatin-resistant (SCC-25) and -sensitive (UPCI-SCC090) HNSCC cell lines, using two specific p38 MAPK inhibitors, SB202190 and ralimetinib. Cell viability was measured by MTT assay; cell cycle distribution and cell death were evaluated by flow cytometry; p38 MAPK phosphorylation, Slug protein stabilization, and p38 MAPK downstream targets were investigated by Western blot. p-p38 inhibitors achieved sustained phosphorylation of p38 MAPK (Thr180/Tyr182) and inhibition of its function, which resulted in decreased phosphorylation (Thr69/71) of the downstream target pATF2 in pEMT cells. Subsequently, the p-p38 inhibition resulted in reduced Slug protein levels. In accordance, p-p38 inhibition led to sensitization of pEMT cells to cisplatin-induced cell death; moreover, p-p38 inhibitor treatment cycles significantly decreased the viability of cisplatin-surviving cells. In conclusion, clinically relevant p38 inhibitors might be effective for RCT-resistant pEMT cells in HNSCC patients.