bluuebunny/crossref_metadata_2025 huggingface dataset:
Sexual dimorphism and sexual selection in cytheroidean ostracodes from the Late Cretaceous of the U.S. Coastal Plain
Authors: Hunt Gene , Martins M. João Fernandes , Puckett T. Markham , Lockwood Rowan , Swaddle John P. , Hall Christine M. S. , Stedman James
Published: August 2017

Sexual dimorphism is common in many extant animals, but it is difficult to demonstrate in fossil species. Working with material from the Late Cretaceous of the U.S. Coastal Plain, we herein analyze sexual dimorphism in ostracodes from the superfamily Cytheroidea, a group whose extant members have males that are relatively more elongate than females. We digitized outlines of more than 6000 individual ostracode valves or carapaces, extracted size (area) and shape (length-to-height ratio) information, and used finite mixture models to assess hypotheses of sexual dimorphism. Male and female clusters can be discerned in nearly all populations with sufficient data, resulting in estimates of size and shape dimorphism for 142 populations across 106 species; an additional nine samples are interpreted to consist only of females. Dimorphism patterns varied across taxa, especially for body size: males range from 30% larger to 20% smaller than females. Magnitudes of sexual dimorphism are generally stable within species across time and space; we can demonstrate substantial evolutionary changes in dimorphism in only one species,Haplocytheridea renfroensis. Several lines of evidence indicate that patterns of sexual dimorphism in these ostracodes reflect male investment in reproduction, suggesting that this study system has the potential to capture variation in sexual selection through the fossil record.
Pierre Cachia 1921–2017
Authors: Ouyang Wen-chin
Published: August 2017

Pierre Cachia slipped away peacefully on 1 April 2017, a few days shy of his ninety-sixth birthday, surrounded by his children and grandchildren. With the passing of this key architect of Arabic studies, those of us who have studied and worked with him will not only mourn the loss of a friend, teacher, and mentor, but also the irretrievable era in which a first generation of postwar American and European Arabists and Orientalists made tremendous strides in fashioning academic studies of modern Arabic literature into what it is today: grounded in native fluency of the Arabic language, informed by real experiences lived in close proximity with Arab writers and storytellers, and took seriously the concerns and priorities of Arab scholars, critics, and intellectuals.
Comparative ROV surveys reveal jellyfish blooming in a deep-sea caldera: the first report of Earleria bruuni from the Pacific Ocean
Authors: Hidaka-Umetsu Mitsuko , Lindsay Dhugal J.
Published: August 2017

Large numbers of the leptomedusa Earleria bruuni were observed inside a semi-closed deep-sea caldera during a comparative survey of the macrozooplankton fauna inside and outside the Kurose Hole, Izu-Ogasawara Islands, by the remotely operated vehicle (ROV) ‘Dolphin-3K’, on 24 September 2000. The Kurose Hole is an inactive volcanic caldera of 790 m bottom depth, located within the Izu-Bonin island arc, south of Tokyo. Archived video and audio data from a dive by the human-occupied vehicle (HOV) ‘Shinkai 2000’, carried out 3 weeks after the ROV dives, was also analysed. During all dives within the caldera, E. bruuni was present in large numbers and, during the HOV dive, two specimens for morphological analysis were obtained. Herein, we report E. bruuni from the Pacific Ocean for the first time. The vertical profiles of environmental factors and the vertical distributions of gelatinous macrozooplankton taxa such as salps, ctenophores, hydromedusae, siphonophores and scyphomedusae, were extremely different inside and outside the caldera. Inside the caldera the water temperatures were warm and dissolved oxygen levels were high compared with outside. For each taxon, their distributions were characterized and compared between the inside and outside of the caldera, and with previous literature reports.
State-dependent microstructural white matter changes in drug-naïve patients with first-episode psychosis
Authors: Serpa M. H. , Doshi J. , Erus G. , Chaim-Avancini T. M. , Cavallet M. , van de Bilt M. T. , Sallet P. C. , Gattaz W. F. , Davatzikos C. , Busatto G. F. , Zanetti M. V.
Published: August 2017

Background

Diffusion tensor imaging (DTI) studies have consistently shown white matter (WM) microstructural abnormalities in schizophrenia. Whether or not such alterations could vary depending on clinical status (i.e. acute psychosisv.remission) remains to be investigated.

Methods

Twenty-five treatment-naïve first-episode psychosis (FEP) patients and 51 healthy-controls (HC) underwent MRI scanning at baseline. Twenty-one patients were re-scanned as soon as they achieved sustained remission of symptoms; 36 HC were also scanned twice. Rate-of-change maps of longitudinal DTI changes were calculated for in order to examine WM alterations associated with changes in clinical status. We conducted voxelwise analyses of fractional anisotropy (FA) and trace (TR) maps.

Results

At baseline, FEP presented reductions of FA in comparison with HC [p< 0.05, false-discovery rate (FDR)-corrected] affecting fronto-limbic WM and associative, projective and commissural fasciculi. After symptom remission, patients showed FA increase over time (p< 0.001, uncorrected) in some of the above WM tracts, namely the right anterior thalamic radiation, right uncinate fasciculus/inferior fronto-occipital fasciculus, and left inferior fronto-occipital fasciculus/inferior longitudinal fasciculus. We also found significant correlations between reductions in PANSS scores and FA increases over time (p< 0.05, FDR-corrected).

Conclusions

WM changes affecting brain tracts critical to the integration of perceptual information, cognition and emotions are detectable soon after the onset of FEP and may partially reverse in direct relation to the remission of acute psychotic symptoms. Our findings reinforce the view that WM abnormalities in brain tracts are a key neurobiological feature of acute psychotic disorders, and recovery from such WM pathology can lead to amelioration of symptoms.
Intergenerational friendships of older adults: why do we know so little about them?
Authors: ELLIOTT O'DARE CATHERINE , TIMONEN VIRPI , CONLON CATHERINE
Published: August 2017

Intergenerational projects bringing together older adults and younger adults are increasingly common, but there is little research on unstructured, naturally occurring interaction, and in particular friendship between different generations. The aim of this article is to interrogate why we know so little about adult intergenerational friendship. A systematic literature search on this topic, covering a 30-year period, yielded only six articles which satisfied the inclusion criteria. This prompted us to examine how the topics of intergenerational friendship and friendship in old age have been approached in the literature to date. We argue that the paucity of research on intergenerational friendship reflects the focus of existing research on homophily, and consequently friendships among olderoryounger adults; and that this in turn reflects a social construction of older adults as unsuited to friendship with younger adults. Investigations of intergenerational friendship can help challenge the images and models of ageing and older adults that both research and societies currently operate with, and are constrained by. We conclude by calling for research that explores the views and experiences of older adults as parties to intergenerational relationships that are non-kin, chosen and based on mutual enjoyment.
Long-term care service needs and planning for the future: a study of middle-aged and older adults in Hong Kong
Authors: HE ALEX JINGWEI , CHOU KEE-LEE
Published: August 2017

Long-term care (LTC) planning is important in helping the older people tackle their future needs better. The needs for LTC services represent generational characteristics as they may be different between the current and upcoming cohorts of older adults. However, very few studies have examined the cohort differences in terms of their expected utilisation of LTC services, while understanding the patterns is crucial in helping policy makers prepare for the development of LTC services. This study fills the research gap by examining the plans and expectations for LTC services of 1,613 middle-aged and older persons in Hong Kong with data collected from a telephone survey. By applying the Andersen Model to examine LTC expectations, this study analyses the LTC needs and plans of the middle-aged and older cohorts of Hong Kong adults, as well as their associated factors, with a multiple logistic regression method. Both gender and birth cohort were examined individually and in combination. Birth cohort and gender have been found to exert an impact on all aspects of LTC needs and planning to varying degrees. The findings are interpreted and contrasted with those of a key study based in the West, with reference to the contextual characteristics of Hong Kong. This study furthers the scholarly understanding on LTC needs and planning and their cohort effect, and draws evidence-based recommendations for LTC development in Hong Kong, a rapidly ageing East Asian society.
Gaga as Metatechnique: Negotiating Choreography, Improvisation, and Technique in a Neoliberal Dance Market
Authors: Quinlan Meghan
Published: August 2017

Gaga, a practice developed by Israeli choreographer Ohad Naharin, is one of the most popular training methods on the global dance market. Structured as a metatechnique, or a system for negotiating techniques within one's body, Gaga teaches students to both draw on and reject multiple movement techniques to create their own movement. I consider how the paradigms of choreography, technique, and improvisation are blurred together in the pedagogical model of a metatechnique and how training dancers to shift between choreographer, dancer, and improviser has significant ramifications for understanding their agency. The metatechnique model of Gaga falls in line with neoliberal values of efficiency and a wide range of skills and knowledge; this analysis provides an understanding of recent trends in dance training in relation to contemporary political and socioeconomic structures.
Temperature-dependent structural behaviour of samarium cobalt oxide
Authors: Rowles Matthew R. , Wang Cheng-Cheng , Chen Kongfa , Li Na , He Shuai , Jiang San-Ping
Published: August 2017

The crystal structure and thermal expansion of the perovskite samarium cobalt oxide (SmCoO3) have been determined over the temperature range 295–1245 K by Rietveld analysis of X-ray powder diffraction data. Polycrystalline samples were prepared by a sol–gel synthesis route followed by high-temperature calcination in air. SmCoO3 is orthorhombic (Pnma) at all temperatures and is isostructural with GdFeO3. The structure was refined as a distortion mode of a parent $ Pm{\bar 3}m $ structure. The thermal expansion was found to be non-linear and anisotropic, with maximum average linear thermal expansion coefficients of 34.0(3) × 10−6, 24.05(17) × 10−6, and 24.10(18) × 10−6 K−1 along the a-, b-, and c-axes, respectively, between 814 and 875 K.
Vitamin D insufficiency/deficiency is associated with insulin resistance in Brazilian children, regardless of body fat distribution
Authors: Milagres Luana Cupertino , Rocha Naruna Pereira , Filgueiras Mariana de Santis , Albuquerque Fernanda Martins de , Castro Ana Paula Pereira , Pessoa Milene Cristine , Gouveia Peluzio Maria do Carmo , Novaes Juliana Farias de
Published: August 2017

Objective

To assess the prevalence of vitamin D insufficiency and deficiency and its association with cardiometabolic risk factors, controlled by adiposity, in a representative sample of prepubescent children.

Design

Cross-sectional population-based study. Body composition was evaluated by dual-energy X-ray absorptiometry. Anthropometric measures and blood pressure were performed. Laboratory analyses were performed to determine the levels of vitamin D (25-hydroxyitamin D; 25(OH)D), glucose, insulin, serum lipids and intact parathyroid hormone. Dietary intake was assessed by three 24 h recalls.

Setting

Viçosa, Minas Gerais, Brazil, 2015.

Subjects

Representative sample of 378 children aged 8 and 9 years from urban schools.

Results

Inadequate serum concentrations of 25(OH)D were diagnosed in more than half of the children and none of them met the recommended vitamin D intake. After adjusting for confounding factors in the multiple regression analysis, lower prevalence of insulin resistance and hypertriacylglycerolaemia was found in children with serum 25(OH)D levels ≥75 nmol/l (prevalence ratio=0·25; 95 % CI 0·08, 0·85) and ≥50 nmol/l (prevalence ratio=0·61; 95 % CI 0·37, 0·99), respectively. However, after adjusting for different indicators of adiposity, insulin resistance remained independently associated and the association with hypertriacylglycerolaemia was lost after adjusting for central adiposity. The prevalence of vitamin D insufficiency/deficiency was associated with the number of cardiometabolic alterations in children.

Conclusions

The study results showed that prevalence of vitamin D insufficiency/deficiency was high among the children and insulin resistance was the main cardiometabolic alteration associated with this condition, even in a tropical climate country such as Brazil.
“We never speak about death.” Healthcare professionals' views on palliative care for inpatients in Tanzania: A qualitative study
Authors: Lewis E. Grace , Oates Lloyd L. , Rogathi Jane , Duinmaijer Ashanti , Shayo Aisa , Megiroo Simon , Bakari Barthlomew , Dewhurst Felicity , Walker Richard W. , Dewhurst Matt , Urasa Sarah
Published: August 2017

Objective:

Little is known about the current views and practices of healthcare professionals (HCPs) in Sub-Saharan Africa (SSA) regarding delivery of hospital palliative care. The present qualitative study explored the views of nursing staff and medical professionals on providing palliative and end-of-life care (EoLC) to hospital inpatients in Tanzania.

Method:

Focus group discussions were conducted with a purposive sample of HCPs working on the medical and pediatric wards of the Kilimanjaro Christian Medical Centre, a tertiary referral hospital in northern Tanzania. Transcriptions were coded using a thematic approach.

Results:

In total, 32 healthcare workers were interviewed via 7 focus group discussions and 1 semistructured interview. Four major themes were identified. First, HCPs held strong views on what factors were important to enable individuals with a life-limiting diagnosis to live and die well. Arriving at a state of “acceptance” was the ultimate goal; however, they acknowledged that they often fell short of achieving this for inpatients. Thus, the second theme involved identifying the “barriers” to delivering palliative care in hospital. Another important factor identified was difficulty with complex communications, particularly “breaking bad news,” the third theme. Fourth, participants were divided about their personal preferences for “place of EoLC,” but all emphasized the benefits of the hospital setting so as to enable better symptom control.

Significance of results:

Despite the fact that all the HCPs interviewed were regularly involved in providing palliative and EoLC, they had received limited formal training in its provision, although they identified such training as a universal requirement. This training gap is likely to be present across much of SSA. Palliative care training, particularly in terms of communication skills, should be comprehensively integrated within undergraduate and postgraduate education. Research is needed to develop culturally appropriate curricula to equip HCPs to manage the complex communication challenges that occur in caring for a diverse inpatient group with palliative care needs.
Iron Supplementation in Twin Pregnancy — The Benefit of Doubling the Iron Dose in Iron Deficient Pregnant Women: A Randomized Controlled Trial
Authors: Shinar Shiri , Skornick-Rapaport Avital , Maslovitz Sharon
Published: August 2017

Objective: To assess the efficacy of doubling the daily dose of iron supplement in iron-deficient women with twin pregnancies. Study design: Using a prospective randomized controlled trial, iron-deficient women with twin gestations were randomized to receive a single or a double dose of daily iron from 16 weeks of gestation until 6 weeks postpartum. The primary outcome was hemoglobin at 32 weeks. Secondary outcomes included ferritin at 32 weeks, hemoglobin during pregnancy and postpartum, birth weights, preterm birth rate, gastrointestinal side effects, intravenous iron administration, and compliance with treatment. Results: Eighty-five and 87 women were randomized to receive one capsule (group A) or two capsules (group B) of 34 mg of ferrous sulfate, respectively. Mean hemoglobin (9.6 g/dL and 9.7 g/dL) and ferritin (8.6 ng/ml and 8.5 ng/ml) were similar in both groups A and B, respectively, at allocation. Hemoglobin in group B was significantly higher from 32 weeks onward, until 6 weeks postpartum. There were no significant differences in any of the secondary outcomes examined. Conclusions: In twin pregnancies complicated by iron deficiency anemia, doubling the dose of iron increases hemoglobin and ferritin without worsening gastrointestinal side effects.
Unveiling bifidobacterial biogeography across the mammalian branch of the tree of life
Authors: Milani Christian , Mangifesta Marta , Mancabelli Leonardo , Lugli Gabriele A , James Kieran , Duranti Sabrina , Turroni Francesca , Ferrario Chiara , Ossiprandi Maria C , van Sinderen Douwe , Ventura Marco
Published: August 2017

Internally transcribed spacer (ITS) rRNA profiling is a novel tool for detailed analysis of microbial populations at low taxonomic ranks. Here we exploited this approach to explore species-level biogeography of the Bifidobacterium genus across 291 adult mammals. These include humans and 13 other primates, domesticated animals, such as dogs, cats, cows, sheep, goats, horses and pigs, and 46 additional species. The collected profiles revealed the presence of 89 putative novel bifidobacterial taxa in addition to 45 previously described species. Remarkably, in contrast to what is currently known for many gut commensals, we did not observe host-specialization among bifidobacterial species but rather their widespread distribution across mammals. Moreover, ITS rRNA profiling of wild relatives of domesticated dogs, rabbits and pigs clearly indicates that domestication and close contact with humans have impacted on the composition of the fecal bifidobacterial population. These data were complemented by analysis of bifidobacterial communities in milk of eight mammalian families, showing that bifidobacteria represent prototypical early gut microbiota members which are inherited by newborns from their lactating mother. Thus this study highlights the role of bifidobacteria as pioneering gut colonizers of a wide range of mammals.
Redox reactions and weak buffering capacity lead to acidification in the Chesapeake Bay
Authors: Cai Wei-Jun , Huang Wei-Jen , Luther George W. , Pierrot Denis , Li Ming , Testa Jeremy , Xue Ming , Joesoef Andrew , Mann Roger , Brodeur Jean , Xu Yuan-Yuan , Chen Baoshan , Hussain Najid , Waldbusser George G. , Cornwell Jeffrey , Kemp W. Michael
Published: August 2017

The combined effects of anthropogenic and biological CO2 inputs may lead to more rapid acidification in coastal waters compared to the open ocean. It is less clear, however, how redox reactions would contribute to acidification. Here we report estuarine acidification dynamics based on oxygen, hydrogen sulfide (H2S), pH, dissolved inorganic carbon and total alkalinity data from the Chesapeake Bay, where anthropogenic nutrient inputs have led to eutrophication, hypoxia and anoxia, and low pH. We show that a pH minimum occurs in mid-depths where acids are generated as a result of H2S oxidation in waters mixed upward from the anoxic depths. Our analyses also suggest a large synergistic effect from river–ocean mixing, global and local atmospheric CO2 uptake, and CO2 and acid production from respiration and other redox reactions. Together they lead to a poor acid buffering capacity, severe acidification and increased carbonate mineral dissolution in the USA’s largest estuary.
Optimization of lattice surgery is NP-hard
Authors: Herr Daniel , Nori Franco , Devitt Simon J.
Published: August 2017

The traditional method for computation in either the surface code or in the Raussendorf model is the creation of holes or “defects” within the encoded lattice of qubits that are manipulated via topological braiding to enact logic gates. However, this is not the only way to achieve universal, fault-tolerant computation. In this work, we focus on the lattice surgery representation, which realizes transversal logic operations without destroying the intrinsic 2D nearest-neighbor properties of the braid-based surface code and achieves universality without defects and braid-based logic. For both techniques there are open questions regarding the compilation and resource optimization of quantum circuits. Optimization in braid-based logic is proving to be difficult and the classical complexity associated with this problem has yet to be determined. In the context of lattice-surgery-based logic, we can introduce an optimality condition, which corresponds to a circuit with the lowest resource requirements in terms of physical qubits and computational time, and prove that the complexity of optimizing a quantum circuit in the lattice surgery model is NP-hard.
Observation of two superconducting domes under pressure in tetragonal FeS
Authors: Zhang Jun , Liu Feng-Liang , Ying Tian-Ping , Li Na-Na , Xu Yang , He Lan-Po , Hong Xiao-Chen , Yu Yun-Jie , Wang Ming-Xiang , Shen Jian , Yang Wen-Ge , Li Shi-Yan
Published: August 2017

We investigate the evolution of superconductivity and structure with pressure for the new superconductor FeS (Tc ≈ 4.5 K), a sulfide counterpart of FeSe. A rapid suppression of T
c
and vanishing of superconductivity at 4.0 GPa are observed, followed by a second superconducting dome from 5.0 to 22.3 GPa with a 30% enhancement in maximum Tc. An onsite tetragonal to hexagonal phase transition occurs around 7.0 GPa, followed by a broad pressure range of phase coexistence. The residual deformed tetragonal phase is considered as the source of second superconducting dome. The observation of two superconducting domes in iron-based superconductors poses great challenges for understanding their pairing mechanism.
Inference on Paleoclimate Change Using Microbial Habitat Preference in Arctic Holocene Sediments
Authors: Han Dukki , Nam Seung-Il , Kim Ji-Hoon , Stein Ruediger , Niessen Frank , Joe Young Jin , Park Yu-Hyeon , Hur Hor-Gil
Published: August 2017

The present study combines data of microbial assemblages with high-resolution paleoceanographic records from Core GC1 recovered in the Chukchi Sea. For the first time, we have demonstrated that microbial habitat preferences are closely linked to Holocene paleoclimate records, and found geological, geochemical, and microbiological evidence for the inference of the sulphate-methane transition zone (SMTZ) in the Chukchi Sea. In Core GC1, the layer of maximum crenarchaeol concentration was localized surrounding the SMTZ. The vertically distributed predominant populations of Gammaproteobacteria and Marine Group II Euryarchaeota (MG-II) were consistent with patterns of the known global SMTZs. MG-II was the most prominent archaeal group, even within the layer of elevated concentrations of crenarchaeol, an archaeal lipid biomarker most commonly used for Marine Group I Thaumarchaeota (MG-I). The distribution of MG-I and MG-II in Core GC1, as opposed to the potential contribution of MG-I to the marine tetraether lipid pool, suggests that the application of glycerol dibiphytanyl glycerol tetraethers (GDGT)-based proxies needs to be carefully considered in the subsurface sediments owing to the many unknowns of crenarchaeol. In conclusion, microbiological profiles integrated with geological records seem to be useful for tracking microbial habitat preference, which reflect climate-triggered changes from the paleodepositional environment.
The Prognostic 97 Chemoresponse Gene Signature in Ovarian Cancer
Authors: Matondo Abel , Jo Yong Hwa , Shahid Muhammad , Choi Tae Gyu , Nguyen Minh Nam , Nguyen Ngoc Ngo Yen , Akter Salima , Kang Insug , Ha Joohun , Maeng Chi Hoon , Kim Si-Young , Lee Ju-seog , Kim Jayoung , Kim Sung Soo
Published: August 2017

Patient diagnosis and care would be significantly improved by understanding the mechanisms underlying platinum and taxane resistance in ovarian cancer. Here, we aim to establish a gene signature that can identify molecular pathways/transcription factors involved in ovarian cancer progression, poor clinical outcome, and chemotherapy resistance. To validate the robustness of the gene signature, a meta-analysis approach was applied to 1,020 patients from 7 datasets. A 97-gene signature was identified as an independent predictor of patient survival in association with other clinicopathological factors in univariate [hazard ratio (HR): 3.0, 95% Confidence Interval (CI) 1.66–5.44, p = 2.7E-4] and multivariate [HR: 2.88, 95% CI 1.57–5.2, p = 0.001] analyses. Subset analyses demonstrated that the signature could predict patients who would attain complete or partial remission or no-response to first-line chemotherapy. Pathway analyses revealed that the signature was regulated by HIF1α and TP53 and included nine HIF1α-regulated genes, which were highly expressed in non-responders and partial remission patients than in complete remission patients. We present the 97-gene signature as an accurate prognostic predictor of overall survival and chemoresponse. Our signature also provides information on potential candidate target genes for future treatment efforts in ovarian cancer.
Hand Posture Modulates Perceived Tactile Distance
Authors: Longo Matthew R.
Published: August 2017

A growing literature shows that body posture modulates the perception of touch, as well as somatosensory processing more widely. In this study, I investigated the effects of changes in the internal postural configuration of the hand on the perceived distance between touches. In two experiments participants positioned their hand in two postures, with the fingers splayed (Apart posture) or pressed together (Together posture). In Experiment 1, participants made forced-choice judgments of which of two tactile distances felt bigger, one oriented with the proximal-distal hand axis (Along orientation) and one oriented with the medio-lateral hand axis (Across orientation). In Experiment 2, participants made verbal estimates of the absolute distance between a single pair of touches, in one of the two orientations. Consistent with previous results, there was a clear bias to perceive distances in the across orientation as larger than those in the along orientation. Perceived tactile distance was also modulated by posture, with increased judgments in both orientations when the fingers were splayed. These results show that changes in the internal posture of the hand modulate the perceived distance between touches on the hand, and add to a growing literature showing postural modulation of touch.
Investigating the Association between Alcohol and Risk of Head and Neck Cancer in Taiwan
Authors: Huang Cheng-Chih , Hsiao Jenn-Ren , Lee Wei-Ting , Lee Yao-Chou , Ou Chun-Yen , Chang Chan-Chi , Lu Yu-Cheng , Huang Jehn-Shyun , Wong Tung-Yiu , Chen Ken-Chung , Tsai Sen-Tien , Fang Sheen-Yie , Wu Jiunn-Liang , Wu Yuan-Hua , Hsueh Wei-Ting , Yen Chia-Jui , Wu Shang-Yin , Chang Jang-Yang , Lin Chen-Lin , Wang Yi-Hui , Weng Ya-Ling , Yang Han-Chien , Chen Yu-Shan , Chang Jeffrey S.
Published: August 2017

Although alcohol is an established risk factor of head and neck cancer (HNC), insufficiencies exist in the literature in several aspects. We analyzed detailed alcohol consumption data (amount and type of alcoholic beverage) of 811 HNC patients and 940 controls to evaluate the association between alcohol and HNC by HNC sites and by genotypes of ADH1B and ALDH2. Alcohol was associated with an increased HNC risk in a dose-response relationship, with the highest risk observed for hypopharyngeal cancer, followed by oropharyngeal and laryngeal cancers. Liquor showed a stronger positive association with HNC than beer and wine. The highest HNC risk occurred in individuals with the slow ADH1B and slow/non-functional ALDH2 genotype combination. In our study population, 21.8% of HNCs, 55.7% of oropharyngeal cancers, and 89.1% of hypopharyngeal cancers could be attributed to alcohol. Alcohol accounted for 47.3% of HNCs among individuals with the slow ADH1B and slow/non-functional ALDH2 genotype combination. The HNC risk associated with alcohol became comparable to that of never/occasional drinkers after ten or more years of cessation from regular alcohol drinking. In conclusion, alcohol use is associated with an increased HNC risk, particularly for individuals with slow ethanol metabolism. HNC incidence may be reduced by alcohol cessation.
Atp6ap2 ablation in adult mice impairs viability through multiple organ deficiencies
Authors: Wendling Olivia , Champy Marie-France , Jaubert Solène , Pavlovic Guillaume , Dubos Aline , Lindner Loic , Jacobs Hugues , Mark Manuel , Combe Roy , Da Cruz Isabelle Goncalves , Luche Hervé , Mudgett John S. , Rosahl Thomas , Sorg Tania , Malissen Marie , Reilly Patrick T. , Hérault Yann
Published: August 2017

ATP6AP2 codes for the (pro)renin receptor and is an essential component of vacuolar H+ ATPase. Activating (pro)renin for conversion of Angiotensinogen to Angiotensin makes ATP6AP2 attractive for drug intervention. Tissue-specific ATP6AP2 inactivation in mouse suggested a strong impact on various organs. Consistent with this, we found that embryonic ablation of Atp6ap2 resulted in both male hemizygous lethality and female haploinsufficiency. Next, we examined the phenotype of an induced inactivation in the adult animal, most akin to detect potential effect of functional interference of ATP6AP2 through drug therapy. Induced ablation of Atp6ap2, even without equal efficiency in all tissues (aorta, brain and kidney), resulted in rapid lethality marked by weight loss, changes in nutritional as well as blood parameters, leukocyte depletion, and bone marrow hypoplasia. Upon Atp6ap2 ablation, the colon demonstrated a rapid disruption of crypt morphology, aberrant proliferation, cell-death activation, as well as generation of microadenomas. Consequently, disruption of ATP6AP2 is extremely poorly tolerated in the adult, and severely affects various organ systems demonstrating that ATP6AP2 is an essential gene implicated in basic cellular mechanisms and necessary for multiple organ function. Accordingly, any potential drug targeting of this gene product must be strictly assessed for safety.
Delayed APC/C activation extends the first mitosis of mouse embryos
Authors: Ajduk Anna , Strauss Bernhard , Pines Jonathon , Zernicka-Goetz Magdalena
Published: August 2017

The correct temporal regulation of mitosis underpins genomic stability because it ensures the alignment of chromosomes on the mitotic spindle that is required for their proper segregation to the two daughter cells. Crucially, sister chromatid separation must be delayed until all the chromosomes have attached to the spindle; this is achieved by the Spindle Assembly Checkpoint (SAC) that inhibits the Anaphase Promoting Complex/Cyclosome (APC/C) ubiquitin ligase. In many species the first embryonic M-phase is significantly prolonged compared to the subsequent divisions, but the reason behind this has remained unclear. Here, we show that the first M-phase in the mouse embryo is significantly extended due to a delay in APC/C activation. Unlike in somatic cells, where the APC/C first targets cyclin A2 for degradation at nuclear envelope breakdown (NEBD), we find that in zygotes cyclin A2 remains stable for a significant period of time after NEBD. Our findings that the SAC prevents cyclin A2 degradation, whereas over-expressed Plk1 stimulates it, support our conclusion that the delay in cyclin A2 degradation is caused by low APC/C activity. As a consequence of delayed APC/C activation cyclin B1 stability in the first mitosis is also prolonged, leading to the unusual length of the first M-phase.
Interleukin-6, tumor necrosis factor-alpha and receptor activator of nuclear factor kappa ligand are elevated in hypertrophic gastric mucosa of pachydermoperiostosis
Authors: Huang Hui , Wang Yongjun , Cao Yong , Wu Boda , Li Yonggui , Fan Liangliang , Tan Zhiping , Jiang Yi , Tang Jianguang , Hu Jianzhong , Shi Xiaoliu
Published: August 2017

Pachydermoperiostosis (PDP) is a rare inherited multisystem disease characterized with digital clubbing, pachydermia and periostosis. Variants in either HPGD or SLCO2A1 that interrupt the prostaglandin E2 (PGE2) pathway have been shown to be involved in PDP. Here, in addition to six confirmed variants in HPGD or SLCO2A1, we identified four novel SLCO2A1 variants in eight PDP patients from seven Chinese Han families. In addition, gastric mucosa hyperplasia was observed in all affected individuals and interleukin-6 (IL-6), tumor necrosis factor-alpha (TNFα) and receptor activator of nuclear factor kappa ligand (RANKL) expression were elevated in hypertrophic gastric mucosa. Two of eight patients who had severe arthralgia were treated with celecoxib. After three months, their arthralgia was partly relieved and IL-6, TNFα and RANKL expression were decreased in accordance with their relieved hypertrophic gastric mucosa. Our study broadens the variation spectrum of SLCO2A1 and suggests that the gastric mucosa hyperplasia might be a common characteristic of PDP. Moreover, celecoxib would be a considerable choice for PDP patients. We also revealed that IL-6, TNFα and RANKL may play important roles in the molecular mechanisms of gastric mucosa hyperplasia in PDP for the first time.
Desorption of hydrocarbon chains by association with ionic and nonionic surfactants under flow as a mechanism for enhanced oil recovery
Authors: Terrón-Mejía Ketzasmin A. , López-Rendón Roberto , Goicochea Armando Gama
Published: August 2017

The need to extract oil from wells where it is embedded on the surfaces of rocks has led to the development of new and improved enhanced oil recovery techniques. One of those is the injection of surfactants with water vapor, which promotes desorption of oil that can then be extracted using pumps, as the surfactants encapsulate the oil in foams. However, the mechanisms that lead to the optimal desorption of oil and the best type of surfactants to carry out desorption are not well known yet, which warrants the need to carry out basic research on this topic. In this work, we report non equilibrium dissipative particle dynamics simulations of model surfactants and oil molecules adsorbed on surfaces, with the purpose of studying the efficiency of the surfactants to desorb hydrocarbon chains, that are found adsorbed over flat surfaces. The model surfactants studied correspond to nonionic and cationic surfactants, and the hydrocarbon desorption is studied as a function of surfactant concentration under increasing Poiseuille flow. We obtain various hydrocarbon desorption isotherms for every model of surfactant proposed, under flow. Nonionic surfactants are found to be the most effective to desorb oil and the mechanisms that lead to this phenomenon are presented and discussed.
Comparison of Continuous Glucose Monitoring between Dexcom G4 Platinum and HD-XG Systems in Nonhuman Primates (Macaca Fascicularis)
Authors: Wang Bingdi , Qiao Wei , Ye Weiwei , Wang Xiaoli , Liu Yongqiang , Wang Yixin , Xiao Yong-Fu
Published: August 2017

Timely knowing glucose level helps diabetic patients to manage the disease, including decisions about food, physical activity and medication. This study compared two continuous glucose monitoring systems in conscious and moving-free nonhuman primates (NHPs,Macaca fascicularis). Each normoglycemic or diabetic monkey was implanted with one Dexcom G4 Platinum subcutaneously or one HD-XG glucose sensor arterially for glucose monitoring. The glucose levels measured by both telemetry devices significantly correlated with the glucometer readings. The data of oral glucose tolerance test (oGTT) showed that the glucose levels measured by either Dexcom G4 Platinum or HD-XG transmitter were very similar to glucometer readings. However, compared to HD-XG transmitter or glucometer, Dexcom G4 Platinum detected a decreased glucose peak of ivGTT with approximately 10 min delay due to interstitial glucose far behind blood glucose change. Our data showed the advantages of the telemetry systems are: (1) consecutive data collection (day and night); (2) no bleeding; (3) no anesthesia (moving freely); (4) recording natural response without physical restriction and stress; (5) less labor intensity during ivGTT and other tests; (6) quick outcomes without lab tests. This article summarized and compared the differences of the general characteristics of two continuous glucose monitoring systems in diabetic research.
Super-Resolved Nuclear Magnetic Resonance Spectroscopy
Authors: Mulleti Satish , Singh Amrinder , Brahmkhatri Varsha P. , Chandra Kousik , Raza Tahseen , Mukherjee Sulakshana P. , Seelamantula Chandra Sekhar , Atreya Hanudatta S.
Published: August 2017

We present a novel method that breaks the resolution barrier in nuclear magnetic resonance (NMR) spectroscopy, allowing one to accurately estimate the chemical shift values of highly overlapping or broadened peaks. This problem is routinely encountered in NMR when peaks have large linewidths due to rapidly decaying signals, hindering its application. We address this problem based on the notion of finite-rate-of-innovation (FRI) sampling, which is based on the premise that signals such as the NMR signal, can be accurately reconstructed using fewer measurements than that required by existing approaches. The FRI approach leads to super-resolution, beyond the limits of contemporary NMR techniques. Using this method, we could measure for the first time small changes in chemical shifts during the formation of a Gold nanorod-protein complex, facilitating the quantification of the strength of such interactions. The method thus opens up new possibilities for the application and acceleration of multidimensional NMR spectroscopy across a wide range of systems.
Klein tunneling near the Dirac points in metal-dielectric multilayer metamaterials
Authors: Sun Lei , Gao Jie , Yang Xiaodong
Published: August 2017

The Klein tunneling of optical waves near the Dirac points in the metal-dielectric multilayer metamaterials is theoretically investigated and demonstrated through the coupled-mode theory under the tight-binding approximation and the rigorous band structure analysis based on the transfer-matrix method. The optical analogue of Klein tunneling for the relativistic electrons passing across a potential barrier is revealed by the iso-frequency contour analysis and numerical simulation to describe the optical beam propagation and refraction across the interface of two metal-dielectric multilayer metamaterial stacks. The transmission and reflection spectra of the Klein tunneling of optical waves are also explained by the coupled mode theory.
Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja)
Authors: Zhang Hengyou , Kjemtrup-Lovelace Susanne , Li Changbao , Luo Yan , Chen Lars P. , Song Bao-Hua
Published: August 2017

Soybean cyst nematode (SCN) is the most damaging pest of soybean worldwide. The molecular mechanism of SCN resistance remains largely unknown. We conducted a global RNA-seq comparison between a resistant genotype (S54) and a susceptible genotype (S67) of Glycine soja, the wild progenitor of soybean, to understand its regulatory network in SCN defense. The number of differentially expressed genes (DEGs) in S54 (2,290) was much larger than that in S67 (555). A number of defense-related genes/pathways were significantly induced only in S54, while photosynthesis and several metabolic pathways were affected in both genotypes with SCN infection. These defense-associated DEGs were involved in pathogen recognition, calcium/calmodulin-mediated defense signaling, jasmonic acid (JA)/ethylene (ET) and sialic acid (SA)-involved signaling, the MAPK signaling cascade, and WRKY-involved transcriptional regulation. Our results revealed a comprehensive regulatory network involved in SCN resistance and provided insights into the complex molecular mechanisms of SCN resistance in wild soybean.
Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing
Authors: Godinho Marta S. C. , Thorpe Chavaunne T. , Greenwald Steve E. , Screen Hazel R. C.
Published: August 2017

Tendon is composed of fascicles bound together by the interfascicular matrix (IFM). Energy storing tendons are more elastic and extensible than positional tendons; behaviour provided by specialisation of the IFM to enable repeated interfascicular sliding and recoil. With ageing, the IFM becomes stiffer and less fatigue resistant, potentially explaining why older tendons become more injury-prone. Recent data indicates enrichment of elastin within the IFM, but this has yet to be quantified. We hypothesised that elastin is more prevalent in energy storing than positional tendons, and is mainly localised to the IFM. Further, we hypothesised that elastin becomes disorganised and fragmented, and decreases in amount with ageing, especially in energy storing tendons. Biochemical analyses and immunohistochemical techniques were used to determine elastin content and organisation, in young and old equine energy storing and positional tendons. Supporting the hypothesis, elastin localises to the IFM of energy storing tendons, reducing in quantity and becoming more disorganised with ageing. These changes may contribute to the increased injury risk in aged energy storing tendons. Full understanding of the processes leading to loss of elastin and its disorganisation with ageing may aid in the development of treatments to prevent age related tendinopathy.
Plasmodium vivax ligand-receptor interaction: PvAMA-1 domain I contains the minimal regions for specific interaction with CD71+ reticulocytes
Authors: Arévalo-Pinzón Gabriela , Bermúdez Maritza , Hernández Diana , Curtidor Hernando , Patarroyo Manuel Alfonso
Published: August 2017

The malarial parasite’s invasion is complex, active and coordinated, involving many low and high affinity interactions with receptors on target cell membrane. Proteomics analysis has described around 40 proteins in P. vivax which could be involved in reticulocyte invasion; few have been studied with the aim of elucidating how many of them establish specific interactions with their respective host cells. Given the importance of knowing which of the parasite’s protein regions are functionally important for invasion, minimum regions mediating specific interaction between Plasmodium vivax apical membrane antigen 1 (PvAMA-1) and its host cell were here elucidated. The region covering PvAMA-1 domains I and II (PvAMA-DI-II) specifically bound to the CD71+ red blood cell subpopulation. A 20 residue-long region (81EVENAKYRIPAGRCPVFGKG100) located in domain I was capable of inhibiting PvAMA-DI-II recombinant protein binding to young reticulocytes (CD71+CD45−) and rosette formation. This conserved peptide specifically interacted with high affinity with reticulocytes (CD71+) through a neuraminidase- and chymotrypsin-treatment sensitive receptor. Such results showed that, despite AMA-1 having universal functions during late Plasmodium invasion stages, PvAMA-1 had reticulocyte-preferring binding regions, suggesting that P. vivax target cell selection is not just restricted to initial interactions but maintained throughout the erythrocyte invasion cycle, having important implications for designing a specific anti-P. vivax vaccine.
Conditions for Barrel and Clam-Shell Liquid Drops to Move on Bio-inspired Conical Wires
Authors: Luo Cheng , Wang Xiang
Published: August 2017

It has been reported that, in a foggy environment, water drops with either barrel or clam-shell shapes are capable of self-running on conical wire-like structures, such as cactus spines, spider silk, and water striders’ legs. On the other hand, the corresponding moving mechanisms are still not quite understood. For instance, it is unclear under what conditions clam-shell drops would move from the tip towards the root on a conical wire. In this work, based on the balance of forces, we derive conditions for a drop to self-transport towards or away from the root. We find that, although barrel and clam-shell drops have different shapes, these conditions are applicable to both of them, which thus provide good guidelines for developing artificial fog collectors. Furthermore, based on the derived conditions, we interpret drop movements on both hydrophilic and hydrophobic wires, with the support of experimental results on cactus spines. Finally, our results indicate that not all the cacti are able to harvest water from fog.
Occurrence of Corynebacterium striatum as an emerging antibiotic-resistant nosocomial pathogen in a Tunisian hospital
Authors: Alibi Sana , Ferjani Asma , Boukadida Jalel , Cano María Eliecer , Fernández-Martínez Marta , Martínez-Martínez Luis , Navas Jesús
Published: August 2017

Corynebacterium striatum is a nosocomial opportunistic pathogen increasingly associated with a wide range of human infections and is often resistant to several antibiotics. We investigated the susceptibility of 63 C. striatum isolated at the Farhat-Hached hospital, Sousse (Tunisia), during the period 2011–2014, to a panel of 16 compounds belonging to the main clinically relevant classes of antimicrobial agents. All strains were susceptible to vancomycin, linezolid, and daptomycin. Amikacin and gentamicin also showed good activity (MICs90 = 1 and 2 mg/L, respectively). High rates of resistance to penicillin (82.5%), clindamycin (79.4%), cefotaxime (60.3%), erythromycin (47.6%), ciprofloxacin (36.5%), moxifloxacin (34.9%), and rifampicin (25.4%) were observed. Fifty-nine (93.7%) out of the 63 isolates showed resistance to at least one compound and 31 (49.2%) were multidrug-resistant. Twenty-nine resistance profiles were distinguished among the 59 resistant C. striatum. Most of the strains resistant to fluoroquinolones showed a double mutation leading to an amino acid change in positions 87 and 91 in the quinolone resistance-determining region of the gyrA gene. The 52 strains resistant to penicillin were positive for the gene bla, encoding a class A β-lactamase. Twenty-two PFGE patterns were identified among the 63 C. striatum, indicating that some clones have spread within the hospital.
Laser-assisted Rapid Mineralization of Human Tooth Enamel
Authors: Sun Muyang , Wu Nier , Chen Haifeng
Published: August 2017

The human body has difficulty repairing damaged dental enamel, an acellular hard tissue. Researchers have sought feasible biomimicry strategies to repair enamel defects; however, few have been successfully translated to clinical applications. In this study, we propose a new method for achieving rapid enamel mineralization under a near-physiological environment. Through treatment with a laser and chelating agents, 15 μm crystals could be grown compactly on an enamel substrate in less than 20 min. The compact crystal layer had similar structure as native enamel prisms and high elastic modulus. This layer also had the potential for further remineralization in saliva. The benefit of using laser can not only speed up the mineralization, but also control the crystal growth precisely where in need. A mechanism for how laser and chelating agents synergistically function is also proposed. This strategy offers a possibility for enamel-biomimicking repair in dental clinics.