bluuebunny/crossref_metadata_2025 huggingface dataset:
New insights to estimate reproduction numbers along an epidemic
Authors: Sebastiani Giovanni , Spassiani Ilaria
Published: April 2022

In this paper, we focus on the problem of estimating the reproduction number Rt along an epidemic, as it represents the most used tool to study and control this phenomenon. In particular, we focus on two issues. First, when dealing with the standard estimator for Rt , we consider the use of positive test data as an alternative to the first symptoms ones, that are typically used. We study both theoretically and empirically the relationship between the two Rt sequences obtained. Second, we describe a new estimator for Rt that is not affected by the drawbacks due to the hypothesis of its local constancy assumed in the standard approach. We illustrate the results obtained by applying the proposed methodologies to both real SARS-CoV-2 Italian data, and two types of simulated ones. In both the two cases, we apply specific methods we have developed to reduce both sistematic and random errors affecting the data. Our results show that the Rt along an epidemic can be estimated with some advantages by using the positive test sequence, and that the estimator we propose outperforms the standard one. We hope that these new methodologies could help to study and control epidemics, and in particular the current SARS-CoV-2 pandemic.
Sulfate Erosion Investigation on FRP-confined Concrete in Cold Region
Authors: ji yongcheng , zou yunfei , li wei
Published: April 2022

Fiber-reinforced polymer (FRP) confined concrete is regarded as an innovative and economical approach for structural repairation. Two typical materials (carbon fiber reinforced polymer (CFRP) and glass fiber reinforced polymer (GFRP)) are selected in this study to investigate the concrete strengthen effect in a severe environment. The resist ability of FRP-confined concrete is discussed when subjected to coupled erosion between sulfate erosion and freeze-thaw cycles. Electron microscopy examines concrete's surface and interior degradation during coupled erosion. The corrosion degree and principle of sodium sulfate are analyzed using pH, SEM electron microscope, and EDS energy spectrum. The axial compressive strength test is used to evaluate the reinforcement of the FRP-confined concrete column, and the stress-strain relationship for various FRP-confined techniques in a coupled erosion environment is obtained. The error analysis is performed to calibrate the experimental test result using four existed prediction models. All observations indicate that the deterioration process of FRP-confined concrete is complicated and dynamic under coupled effect. Sodium sulfate initially increases the initial strength of concrete. However, subsequent freeze-thaw cycles may aggravate concrete fractures, while sodium sulfate further degrades the strength of concrete through the cracking development. A precise numerical model is presented to simulate the stress-strain relationship, which is critical for the design and life cycle assessment of FRP-confined concrete.
Epigenetic genes alterations in metastatic solid tumors: results from the prospective precision medicine MOSCATO and MATCH-R trials
Authors: Postel-Vinay Sophie , Martin-Romano Patricia , Colmet-Daage Leo , Morel Daphne , Baldini Capucine , Verlingue Loic , Bahleda Rastiav , Gazzah Anas , Champiat Stephane , Varga Andreea , Michot Jean Marie , Ngo-Camus Maud , Nicotra Claudio , Marabelle Aurelien , Soria Jean Charles , Rouleau Etienne , Lacroix Ludovic , Hollebecque Antoine , Massard Christophe
Published: April 2022

Purpose: Although the role of epigenetic alterations in oncogenesis has been well studied, their prevalence in metastatic solid tumors is still poorly described. We therefore aimed at: (i) describing the presence of epigenetic gene alterations (EGA) - defined by an alteration in a gene encoding an epigenetic regulator; and (ii) evaluating their relationship with clinical characteristics and outcome in patients (pts) included in prospective molecular profiling trials.Patients and methods: On-purpose tumor biopsies from pts with metastatic solid tumors enrolled in the Gustave Roussy-sponsored MOSCATO (NCT01566019) and MATCHR (NCT02517892) trials were molecularly profiled using Whole Exome Sequencing (WES). Alterations in 176 epigenetic genes were assessed and classified as pathogenic variants (PV) or non-pathogenic variants by a molecular tumor board. Clinical characteristics and outcome were collected.Results: Between Dec 2011 and Oct 2016, WES was successfully performed in 292 pts presenting various solid tumors. We found 496 epigenetic gene alterations in 134 patients (49%), including 237 pathogenic variants in 86 patients; 63 tumor samples (47%) presented ≥3 EGAs. The median number of previous treatment lines was 3 (1 – 10). The most frequently altered genes were KMT2D and KMT2C (16% each), ARID1A and SETD2 (10% each) and KMT2A (8%).; 31% of EGA co-occurred with a driver gene alteration (p < 0.001). Outcome was not correlated with the presence of EGA.Conclusions: Epigenetic alterations occur frequently in metastatic solid tumors. With the current development of epigenetic modifiers, they increasingly represent actionable targets. Such genes should now be systematically analyzed in molecular profiling studies.
LC-MS proteomic profiling of trace FFPE samples in gastrointestinal cancer
Authors: Guo Chunmei , Li Lingling , Liu Hui , Ding Chen
Published: April 2022

Background: Early screening and diagnosis of gastrointestinal cancer would be benefit for patients’ prognosis. However, the complexity of cancer progression and the extreme trace amount of tissue samples in different stages have limited in portraying the characterization of early-stage cancer. Here, We disclosed the biological pathways and built an integrated proteomic map of trace FFPE samples in gastrointestinal cancer.Methods: In this study, a quantitative proteomic method with chromatography with mass spectrometry (LC-MS/MS) was used to analyse the different amounts of peptides between the trace duodenum and gastric cancers. Results: We performed a comprehensive proteomic landscape of trace formalin-fixed, paraffin-embedded (FFPE) samples with 5 different amounts of peptides (AOPs) in 40 gastrointestinal cancer samples. Proteomic analysis disclosed the different features of high/low proteins in duodenum and gastric cancers, and revealed the gradually decreased proteins number with the dilutions of AOP. In addition, we found the high coverage of identified proteins when the input of AOPs were no less than 0.5 µg. Proteomic methods represented the loss of tumor heterogeneity when the input of AOPs were no more than 0.5 µg. We also illustrated the dominant pathways in duodenum cancer and gastric cancer, and found the loss function when the input of AOPs was no more than 0.5 µg. Collectively, our study built an integrated proteomic map of trace FFPE samples in gastrointestinal cancer, and provided a valuable resource for clinical researches, especially for early-stage cancer.Conclusion: We disclosed the biological pathways of the trace samples, and revealed the high coverage (> 60%) of identified proteins when the input of AOPs was no less than 0.5 µg, keeping the primary functions of tissues. These findings provided a new insight in early-stage cancer in the clinic.
An Efficient Framework for Video Documentation of Bladder Lesions for Cystoscopy: A Proof-of-Concept Study
Authors: Eminaga Okyaz , Ge T. Jessie , Shkolyar Eugene , Laurie Mark A. , Lee Timothy J. , Hockman Lukas , Jia Xiao , Xing Lei , Liao Joseph C.
Published: April 2022

The identification of relevant frame sequences in an endoscopy video is labor-intensive and complicated by the poor data integrity of endoscopic information. We designed a surgeon-guided framework to efficiently manage the visual data from commonly performed bladder endoscopy (cystoscopy) videos. Screenshots of bladder lesions were captured during cystoscopy during transurethral resection of bladder tumor, then manually labeled according to case identification, date, lesion location, imaging modality, and pathology. The framework used the screenshot to search for and extract a corresponding 10-second video clip. Each video clip included a one-second space holder with a QR barcode informing the video content. The success of the framework was measured by the secondary use of these short clips and the reduction of storage volume required for video materials. From 86 cases, the framework successfully generated 249 video clips from 230 screenshots, with 14 erroneous video clips from 8 screenshots excluded. The HIPPA-compliant barcodes provided information of video contents with a 100% data completeness. A web-based educational gallery was curated with various diagnostic categories and annotated frame sequences. Compared with the unedited videos, the informative short video clips reduced the storage volume by 99.5%. In conclusion, our framework expedites the generation of visual contents with surgeon’s instruction for cystoscopy and potential incorporation of video data towards applications including clinical documentation, education, and research.
Light Absorptance of Algal Films for Photosynthetic Rate Determinations
Authors: RITCHIE Raymond J. , SMA-AIR Suhailar , RUNCIE John W.
Published: April 2022

A Reflectance Absorptance Transmittance (RAT) meter, developed for routine measurements of Abtλ nm on leaves, was used to measure absorptance of algal films mounted on glass fibre filter disks for use in PAM (Pulse Amplitude Modulation) fluorometry. Absorptance readings (Abtλ nm) are essential to quantitate the photosynthetic electron transport rate from PAM data and are also helpful if algal disks are used in an IRGA (InfraRed Gas Analyzer). Measurements were made on Synechococcus R-2 (Cyanophyceae), Acaryochloris marina (Chlorobacteria), Chlorella vulgaris (Chlorophyceae), Chaetoceros sp. (Bacillariophyceae) and Isochrysis sp. (Haptophyta) in blue (445 nm), green (525 nm) and red (625 nm) light. Plots of Abtλ vs. Chl a (mg Chl a m− 2) or Chl d for Acaryochloris (mg Chl d m− 2) of the algae followed exponential saturation curves (Abt%λ = Abt%λ, ∞ (1-e− k×Chl)) usually reaching asymptotes in the blue light used by blue-diode PAM machines. Synechococcus, Chlorella, Chaetoceros and Isochrysis Abt%445nm saturates ≈ 50–100 mg Chl a m− 2 (Synechococcus: Abt%445 nm, max = 77.3 ± 0.94, ± 95% conf. limits, Chlorella: Abt%445 nm, max = 77.0 ± 2.06, Chaetoceros: Abt%445 nm, max = 80.6 ± 1.87; Isochrysis: 83.2 ± 8.06 mg Chl a m− 2); Acaryochloris above about 100 mg Chl d m− 2. Asymptotic loading of cells was in general easily achieved in blue light in the case of Synechococcus, Chlorella and Acaryochloris but not in green, red or “white” light. It was difficult to achieve asymptotic loading for Chaetoceros and Isochrysis due to mucilaginous blockage of the filter disks.
Quantitative proteomic analysis to understand the role of Arabidopsis thaliana LEAFY COTYLEDON 2 promoting lipid accumulation in Chlorella sorokiniana by upregulating photosynthetic proteins and G3PDH
Authors: Liu Changbin , Liu Yilin , Yu Shanshan , Dong Wenhao , Xue Yongchang
Published: April 2022

Microalgae attracted significant attention towards biofuel production and transcription factors that can effectively regulate lipid accumulation in higher plants. In this study, we expressed the transcription factor LEAFY COTYLEDON 2 from Arabidopsis thaliana on Chlorella sorokiniana. The electron transport rate (ETR) of C. sorokiniana-AtLEC2 improved significantly under high-intensity light. As a result, the total lipid content and main fatty acid composition (C16, C18) increased by more than one fold. Differentially expressed proteins were analyzed through a label-free strategy to elucidate the regulation mechanism of AtLEC2. The results showed upregulation of TAG biosynthesis (G3PDH) and photosynthesis-related proteins (e.g., ATP synthase F0, Photosystem II, Photosystem I P700, Cytochrome b6, Cytochrome b6-f, Chlorophyll a-b binding protein, Photosystem I assembly protein). Furthermore, AtLEC2 expression in C. sorokiniana led to fixed carbon flow in photosynthesis to TAG through G3PDH upregulation. The current study will provide a new direction towards cultivating high-yield oil algae and reveal the regulation mechanism of AtLEC2 on TAG accumulation in C. sorokiniana.
Intraocular Lens Placement in the Absence of Capsular Support: Visual Outcomes and Complications in a Philippine Tertiary Hospital
Authors: Torrefranca Aramis B. , Cariño Noel S.
Published: April 2022

Purpose:Surgical correction of aphakia without capsular support continues to be a challenge. Rapid rise in technology has provided advancements in techniques in surgical management of aphakia. Locally, we have limited data on the outcomes of the different techniques. This study aimed to determine visual outcomes and complications associated with different techniques of intraocular lens implantation in the absence of capsular support. Setting: The study was conducted at the Philippine General Hospital in Manila, Philippines.Design: This was a retrospective chart review study in a single center.Methods:We reviewed the medical charts of 207 patients who underwent intraocular lens implantation without capsular support. Excluded were patients with incomplete follow up, pediatric patients, and lost records. Best corrected visual acuity at day 1, 1st month, 3rd month and 6th month postoperatively, and the complications were noted. Results:Mean age was 60 with 51% (n=105) were females. Loss of capsular support was most frequently caused by intraoperative complication (n=146, 70%) and trauma. Retropupillary fixation iris claw intraocular lens was frequently used (n=93, 44.9%). Across all patients, visual acuities showed excellent outcomes with 20/50 or better. The presence of dense cataracts (p=0.0088) was associated with postoperative complications. Across IOL types, the most frequent postoperative complication was increase in IOP. Statistically significant results were set at P <0.05.Conclusion:Intraocular lenses used in aphakia showed good postoperative visual acuities. Complications include elevated intraocular pressures, corneal edema, and pigment dispersion. Knowledge on the outcomes maximizes the chances of optimal surgical and visual outcomes.
Outcomes of Sliding Hip Screw Fixation in Stable versus Unstable Intertrochanteric Hip Fractures
Authors: Assad Waleed , Younmis Manaf , Abuodeh Yousef , Shiraz Salman , Ahmed Ghalib
Published: April 2022

Background: Unstable intertrochanteric (IT) fractures are common orthopedic injuries in elderly with high morbidity. The treatment of unstable IT fractures with Sliding Hip Screw (SHS) is still controversial. The purpose of our study is to assess the outcomes of conventional SHS in unstable IT fractures in comparison to stable ones.Methods: Thirty-five stable and 23 unstable IT fractures retrospective cases treated with SHS between January 2017 and December 2018. Patients were evaluated for infection, revision rate, screw cut-out, the development of post-traumatic arthritis or heterotopic ossification and mortality. Results: Mean age of the patients at the time of surgery was 69.8 and 73.9 years for stable and unstable IT fractures, retrospectively, with 38 males and 20 females. The mean follow-up of 45.9 weeks. We found significant association between occurrence of SHS screw cutout and unstable fractures compared to stable ones (0% versus 17.4%, respectively, p = 0.01). There was an obvious trend for non-union and higher revision rate in unstable IT fractures (17.4% vs. 2.9%, p = 0.056). Logistic regression analysis didn’t reveal any statistically significant confounder that might affect the rates of fracture union or revision rates. Post-traumatic arthritis and heterotopic ossification occurred in one case of unstable IT fracture. There were no reported cases of infection.Conclusion: There is high risk of screw cutout, nonunion and revision using the SHS in unstable intertrochanteric fractures (AO type A2.2 and A2.3) compared to stable ones (AO type A2.1). Therefore, other modalities are preferred for the fixation of unstable IT fractures. Level of Evidence: III
Calculation of the Passive Earth Pressure of the Finite Soil behind Retaining Walls Based on Simplified Layering
Authors: Wang Weiwei , Liu Xinxi , Li Bin , Hu Weidong , Luo Hua
Published: April 2022

To investigate the passive earth pressure of finite soil behind retaining walls, this study considered the principal stress deflection caused by the arching effect of soils. To this end, the shape of the principal stress trajectory was simplified to a straight line, and the finite soil behind retaining walls was divided into several thin-layer elements along the simplified straight line. Subsequently, the calculation formulas for the passive earth pressure distribution, resultant force, and height of its action point were obtained based on the limit equilibrium condition of the thin-layer elements. In addition, the correctness and rationality of the proposed method were verified by comparing its values to those of previously reported experiments and theories. The parameter analysis revealed that the resultant force of passive earth pressure decreased significantly at the initial stage with an increase in the aspect ratio of the finite soil and then remained constant, whereas the height of the action point of resultant force increased gradually and then remains unchanged.
Combined effect of microbially-derived caecal SCFA and host genetics on feed efficiency in broiler chickens
Authors: He Zhengxiao , Liu Ranran , Wang Qiao , Zheng Jumei , Ding Jiqiang , Wen Jie , Fahey Alan G , Zhao Guiping
Published: April 2022

Background
Improving feed efficiency is the most important goal for modern animal production. The regulatory mechanisms of controlling feed efficiency traits are extremely complex and include the functions related to host genetics and gut microbiota. Short-chain fatty acids (SCFAs), as significant metabolites of microbiota, could be used to refine the combined effect of host genetics and gut microbiota. However, the association of SCFAs with the gut microbiota and host genetics for regulating feed efficiency is far from understood.
Results
In this study, we examined host genome sequence, microbial data and SCFA concentration of caecal chyme in 300 broilers. The SNP-based heritability analysis found that the SCFA concentrations had moderate to high heritability (h2 = 0.183 ~ 0.401). Genome-wide association studies (GWAS) showed that four out of seven SCFAs had significant associations with genome variants. One locus (gga4: 29414391–29417189) was significantly associated with propionate, locating near or inside the genes MAML3, SETD7 and MGST2, and had a modest effect on feed efficiency traits and the microbiota. The genetic effect of the top SNP explained 8.43% phenotype of propionate. Individuals with genotype AA had significantly different propionate concentrations (0.074 vs. 0.131 ug/mg), feed efficiency (FCR: 1.658 vs. 1.685), and relative abundance of 14 taxa compared to those with the GG genotype. Christensenellaceae and Christensenellaceae_R-7_group were identified being associated with feed efficiency, propionate concentration and top SNP genotypes, and they were found to be lipid metabolism-related. Individuals with a higher caecal abundance of these taxa showed better feed efficiency and lower concentrations of caecal SCFAs.
Conclusion
Our study concluded that host genetic variation could affect the regulation of caecal microbially-derived SCFAs, which plays a role in host feed efficiency traits. The caecal taxa Christensenellaceae and Christensenellaceae_R-7_group were identified as representative taxa contributing to the combined effect of host genetics and SCFAs on chicken feed efficiency. These findings proved strong evidence of the combined effect of host genetics and gut microbial SCFAs in regulating feed efficiency traits.
CHRDL2 promotes cell proliferation by activating the YAP/TAZ signaling pathway in gastric cancer
Authors: Wang Lingquan , Xu Wei , Mei Yu , Wang Xufeng , Liu Wentao , Yan Chao , Li Chen , Zhu Zhenggang , Ni Zhentian
Published: April 2022

The encoding product of Chordin-like 2 (CHRDL2) is a member of the chordin family of proteins, which has been shown to be aberrantly expressed in several types of solid tumors. The regulatory underlying mechanisms of CHRDL2, however, remain poorly understood in gastric cancer (GC). In the present study, we determined that CHRDL2 was abnormally upregulated in human gastric cancer tissues compared with adjacent normal tissues. We also showed that CHRDL2 was positively associated with T stage, the pathological stage, distant metastasis, and poor patient prognosis. Moreover, we verified that overexpressing CHRDL2 promoted the proliferation and cell cycle transition of GC cells both in vitro and in vivo, whereas the opposite results were observed in CHRDL2-depleted cells. In addition, the expression levels of Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) were increased, and the levels of MST2 were decreased in CHRDL2 overexpressing cells. Consistent with previous findings, we observed the converse results in CHRDL2-silenced GC cells. Additionally, knockdown of YAP and overexpression of STK3 (MST2) could reverse the effects of CHRDL2 overexpression-induced proliferation of GC cells in vitro. Taken together, CHRDL2 plays a key role by activating the Hippo/YAP pathway in gastric cancer. Therefore, CHRDL2 could serve as a potential therapeutic tool for the treatment of gastric cancer.
An effect of digital rehabilitation on patients with total hip arthroplasty during the first-month hospital stay in China
Authors: Zhang Chi , Zhang Xin , Zhang Zhean , Ferland Amanda , Li Hua , Yang Yu , Ding Lu
Published: April 2022

Background and Objectives: The video-based physical therapy has shown the promising enhancements in rehabilitation from total hip arthroplasty in the past decade. However, the critical question was whether patients 50 years old and above in China could follow the instructions from mobile application well? This study attempted to answer this question. Methods: One hundred twenty patients with total hip arthroplasty participated in this study. They are randomly assigned into two groups: digital physical therapy (DPT) and conventional physical therapy (CPT) group. These two groups received similar exercise/balance interventions based on but not limited to the suggestions from the American Academy of Orthopedic Surgery in the first-month hospital stay. Five measures were used as follows: Lower Extremities Functional Score, Harris Hip Score, Ten-meter walking test, Timed-Up & Go, and Berg Balance Scale. Results: Compared to CPT group, DPT group demonstrated similar or even better performance in the abovementioned measure, specifically in Berg Balance Scale (p = 0.005). Conclusions: The results clearly showed that DPT was a feasible rehabilitation for future home-based rehabilitation for patients with total hip arthroplasty. Furthermore, our results also demonstrated that the DPT might be more effective than CPT in China due to the unlimited training opportunities after THA. In addition, it would be worthwhile to investigate the cost-effectiveness of this DPT.
Website Reorganization using Physics based Algorithms
Authors: Kumar Santosh , Goyal Aman Kumar , Sharma Arjun , Verma Arpit
Published: April 2022

This is the era of the internet and more than 50 percent of the population is dependent on it. Internet is collection of web pages which can be transported across it. Its size is very huge and increasing exponentially day by day. Now, around 30 percent of the overall content is duplicate. Finding relevant and required information from such a huge source of information is a very challenging task and finding top ranked results is a NP-hard problem. Majorly, the overall content can be categorized in three parts: content data, usage data and structure data. Web mining techniques and metaheuristic algorithms have been successfully applied in the literature to extract the useful information. This paper aims to provide ranking to the web links which could be utilized for restructuring of websites for business intelligence. Different features like keywords frequency, user’s navigation behaviour like unique visitors, duration stayed, access frequency, hub, and authority information has been utilized for prioritizing top-T web links. These results may be useful in different applications that include web personalization, website reorganization, recommendation systems, search engine optimization, etc. Other than this, it will also help in improving the user’s experience on websites. Based on different types of data, algorithms WLRGA, WLRBBBC, and WLRGbSA have been proposed for finding top-T web links and experimentally it has been seen that WLRGbSA is able to select top quality web links useful specially for website reorganization.
Donor hematopoietic stem cell/lymphocyte as maintenance treatment after CAR T-cell therapy in B-cell acute lymphoblastic leukemia relapsed after stem cell transplant
Authors: LI Qing , Cuicui LYU , DENG Haobin , LIU Meijing , Jia WANG , Jijun YUAN , Haibo ZHU , JIANG Yanyu , Erlie JIANG , ZHANG Rongli , DENG Qi
Published: April 2022

Background: Maintaining the efficacy of anti-CD19 chimeric antigen receptor modified (CAR) T-cell therapy in patients with B-cell acute lymphoblastic leukemia (B-ALL) relapsed after transplantation is an urgent problem.Methods: Overall 22 B-ALL patients who relapsed after allogenic hematopoietic stem cell transplantation (allo-HSCT) received anti-CD19-CAR T-cell therapy. Patients who responded to CAR-T cell therapy received donor hematopoietic stem cell infusion (DSI) or donor lymphocyte infusion (DLI) as maintenance therapy. We compared the clinical responses, acute graft versus host disease (aGVHD), expansion of CAR-T cells, and adverse events between two groups. Results: CR or CR with incomplete count recovery was achieved in 19 patients (86.4%). After DSI/DLI therapy, grade I-II of aGVHD was observed in 4 patients (36.4%) in DSI group. Only one patient developed grade II aGVHD in DLI group. The peaks of CAR T-cells in DSI group were higher than in DLI group. IL-6 and TNF-α levels increased again in 9 of 11 patients after DSI but not in DLI group. Progression-free survival and overall survival were higher in DSI group than in DLI group at 365 days. Conclusions: For B-ALL patients who relapsed after allo-HSCT, DSI is a feasible maintenance therapy if CR is obtained with CAR-T cell therapy.Trial registration: ChiCTR-ONN-16009862 Registered14November2016,https://www.chictr.org.cn/showproj.aspx?proj=16756ChiCTR1800019622 Registered24November2018,https://www.chictr.org.cn/showproj.aspx?proj=33185
Involvement of miR-31, miR-148a, & miR -221 as three risky microRNAs in the invasion and angiogenesis of glioblastoma cells via myelinated nerve fiber of white matter tracts path
Authors: Shahkileh Seyedeh Sahar Valinejad , Ghaderian Sayyed Mohammad Hossein , Sheikhha Mohammad Hasan , Mohammadpour Hadiseh , Rakhshan Azadeh , Bastami Milad , Vojdani Samaneh
Published: April 2022

Glioblastoma is one of the most frequently occurring and malignant brain tumors. Due to the importance of microRNAs and HIF1α gene that is involved in the angiogenesis and growth of tumor cells, this study aimed to evaluate the correlation of the expression of HIF1α gene with miR-148a, miR-31, and miR-221, which were reported by in silico analysis as risky microRNAs in GBM. The findings obtained from Real-Time PCR using TaqMan Assay indicated the significant difference in the expression of HIF1α gene and microRNAs between the patient and healthy groups and the expression changes were obtained point to point in the patients; so they can be considered as important biomarkers in glioblastoma. The obtained AUC from the ROC analysis indicated that the analysis of the expression of HIF1α and miR-148a, miR-31 & miR-221 genes can be used to distinguish healthy and patient groups nevertheless, the correlation of HIF1α gene expression with the microRNAs was reported low according to Pearson’s correlation coefficient. Thus the significant increase in HIF1α gene expression is probably controlled by other molecules. By using bioinformatics analysis; Gene Ontology biological process; MSigDB Hallmark and KEGG pathway enrichment analysis of PPI network was carried out with Enrichr web-based application. The axon guidance pathway, proteoglycan in cancer and some of intracellular signaling pathways identified as the most important signaling pathways. The results of MSigDB_Hallmark pathway enrichment analysis introduced hypoxia signaling pathway with less involvement from these micoRNAs. Therefore these microRNAs can be considered in Glioblastoma cell infiltration through the myelinated nerve fibers of white matter tracts.
A Retrospective study on constrictive pericarditis and suggestions on diagnostic criteria
Authors: Li Junfang , Li Rong , Cheng Guangting , Lu Changhong , Liu Weigang , Sun Dongmei , Li Xue , Wang Zhibin
Published: April 2022

Background: The diagnosis of constrictive pericarditis (CP) is a challenge. There is no standard echocardiographic diagnosis criteria of CP yet. This study analyzed and summarized the features of 25 patients with CP, and then proposed suggestions of diagnostic criteria. It is hoped that the suggestions may help professionals make decisions in their daily practice, and patients get timely diagnosis and effective treatment.Methods: Twenty-five patients with CP were selected for the retrospective study. The clinical and echocardiographic imaging data were analyzed and summarized. At end of the article, suggestions on diagnostic criteria of CP were put forward.Results: The main clinical manifestations were fatigue, breathlessness and exertional dyspnea (88%) and signs of edema of lower limbs (84%), hepatomegaly and jugular vein filling (84%). Echocardigraphic features included pericardial thickening (88%) and calcification (60%), pulmonary hypertension (52%), IVC dilation (80%) , left and/or right atrial enlargment (100%), diastolic flattening of the LV posterior wall (72%), septal shudder and bounce (64%), restrictive LV and RV diastolic filling pattern (100%), early filling changes of mitral and tricuspid flow (80% and 60% respectively), mitral annulus reversus (73%).Conclusions: Echocardiography is a simple and valuable examination for CP. The echocardiographic diagnostic criteria are valid and might be further promoted.
Impact of the Covid-19 pandemic on emergency department visits and hospital admissions in a tertiary hospital in Barcelona, Spain.
Authors: Aldecoa Victoria , Madrid Paula , Cahis Nuria , Ranera Adrian , Moya Sandra , Baena Irene , Corripio Raquel
Published: April 2022

BACKGROUND: describe the changes observed in admissions and visits to the pediatric emergency department (ED) due to the lockdown during the COVID-19 pandemic. METHODS: cross-sectional observational study on pediatric ED and hospital admissions comparing two periods (March 9th-May 9th, 2019 and March 9th-May 9th, 2020). RESULTS: 6568 ED visits (53.9% male; mean age 6.5±5.1) were recorded in 2019 and 1133 (55.2% male; mean age 7.3±5.6) in 2020, showing a decrease of 82.7% (81.8 – 83.7%). Severity of patients, procedures and length of stay in ED were significantly higher during the COVID-19 period. Although hospital admissions decreased during the lockdown (-55.1%, p<0.001); 7.9% of ED patients were admitted to the hospital over the study period in 2020 vs 3.0 % in 2019; OR=2.61 (2.03 – 3.35), p<0.001. Hospital admissions related to infectious diseases decreased; OR=0.57 (0.33 – 0.95) and trauma and surgery increased; OR=1.99 (1.16 – 3.41), length of stay remained stable. CONCLUSION: the effects of lockdown during the COVID19 pandemic were translated into a significant decrease of ED visits (83%) and hospital admissions (56%), although hospital admission rate increased (OR=2.61). Hygienic measures, managing symptoms at home and fear of coming to the hospital could explain these changes. Telemedicine and public health education interventions are mandatory in times of lockdown, as well as coordination between primary health care centers and hospitals.
Improving Hadoop Performance through scheduling and prefetching
Authors: EDDOUJAJI MOHAMED , SAMADI HASSAN , BOUHORMA MOHAMMED
Published: April 2022

In this paper, we continue investigating how to improve Hadoop performance , we will investigate further strategies, tackling the data locality with respect to data access patterns, cluster memory and effective scheduling. In a Hadoop cluster, users are generally accessing data according to their business needs, which make some data more accessed than others, reason why we consider data patterns as a crucial element in our approach. In fact, the performance can be affected by the latency of data access, which is best when blocks are already in memory while requested for processing, knowing that caching too much can lead to memory overhead or even severe delays when wrong data are always taking memory space. Actually, clusters are nowadays offering more capabilities in term of nodes memory, which is considered in most cases as highly underutilized according to several studies. In our approach we considered the cluster memory to improve efficiency by using the underutilized memory space in a way to offer better data locality for future tasks, through a new prefetching/scheduling algorithm. The idea behind our approach is to differentiate blocks by popularity based on the previous access patterns, then proceed by memory allocation or memory eviction in optimal and efficient way across all the cluster.
Design and Fabrication of Sierpinski Square Fractal Microstrip Patch Antenna with Parasitic Elements for Wideband Applications
Authors: Kattimani Bharamappa , Patil Rajendra R
Published: April 2022

The narrow bandwidth of a microstrip antenna is one of its key drawbacks. This work shows how a dual-layer stacked and parasitic arrangement, together with the effect of fractal designs, can provide wide bandwidth and high gain qualities in a microstrip patch antenna. For wideband applications, the proposed antenna design in stacked construction has a total bandwidth of 750MHz for S11 -10dB. In order to reduce the antenna size, the Sierpinski square carpet fractal is used. This antenna exhibits a lower resonance frequency due to the space-filling property of fractal geometry. The resonance frequency of this patch antenna drops as iteration and iteration factors rise, according to experimental results. Wideband functioning is achieved with a size decrease of 70.1%. The proposed antenna design exhibits a 6.98dB gain. These qualities of wideband and higher gain make the proposed antenna an excellent one for wideband applications.
Nondipolar photoionization of atoms and molecules
Authors:   , Grundmann Sven Eric
Published: April 2022

The present work deals with photoionization in the realm of the absorption of one single photon. The formal treatment of one-photon ionization usually employs a semi-classical approach, where the electron’s initial and final states are described as quantum-mechanical wave functions but the photon is treated as a classical electromagnetic wave. In the calculation of photoionization cross sections with this semi-classical method, there is an often used approximation which is called the electric dipole approximation. Mathematically, the application of the dipole approximation corresponds to truncating the series expansion of an exponential after the leading term. Physically, this means neglecting the linear photon momentum and the spatial dependence of the light field. The dipole approximation is valid if the wavelength of the light is much larger than the spatial extent of the target and if the photon momentum is small compared to the momenta of the reaction products, which is generally the case for photon energies short above the electron binding energy. For the present work, we experimentally investigated nondipolar photoionization, i.e., one-photon ionization at high photon energies where the dipole approximation breaks down. In our experiments, we irradiated single atoms and molecules with such high-energetic photons and measured the three-dimensional momentum distributions of the reaction fragments to uncover the effects of the linear photon momentum and the spatially-dependent light field on photoionization. Our observations allow the first profound insight into photoionization that reveals all photon properties, i.e., photon energy, spin, linear momentum, and the speed of light. Hopefully, our efforts make a constructive contribution to the understanding and the further exploration of light-matter interaction.
EXOSOMAL CAVEOLIN-1 AS A BIOMARKER OF BLADDER CANCER IN EGYPT
Authors:   Department of Pharmacology &Toxicology and Biochemistry, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University, New Cairo, Egypt., El Sabah Mahmoud , El Sharkawi Fathia ,   Department of Biochemistry and Molecular Biology. Faculty of Pharmacy, Helwan University, Cairo, Egypt., Shabayek Marwa ,   Department of Pharmacology &Toxicology and Biochemistry, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University, New Cairo, Egypt., Khaled Hussein ,   National Cancer Institute, Cairo University, Egypt.
Published: April 2022

Back ground: Cystoscopic examination and histological evaluation of bladder tissues are considered the gold standard for initial diagnosis of bladder cancer. For decades, researchers explore novel, non-invasive, specific and sensitive biomarkers for preliminary diagnosis and surveillance of bladder carcinoma. Exosomes are nano-sized vesicles present in various biological fluids and encapsulate huge number of biomarkers such as proteins, mRNAs and miRNAs. Caveolin-1(Cav-1) is a major protein of caveolae structure that expressed in a variety of cells and has a fundamental regulatory role in cancer development. Therefore, determination ofexosomal Cav-1 expression in different stages of bladder cancer is a critical concern for clinical diagnosis and prognosis. Methods: Exosomes Cav-1 was isolated and extracted from urine and serum samples of 79 patients of bladder cancer at different stages (T0:T3) and 12 healthy controls.Exosomal Cav-1 expression levels were determined by using Rt-qPCR technique. Results:Exosomal Cav-1expression levels at different stagesof bladder cancer patients were significant in both urine and serum samples. The current study showed upregulation ofCav-1 amongserum (4.03, 9.20, 14.25, 26.92folds) and urine samples (2.55, 3.64, 8.33, 19.37folds) comparative to tumor invasiveness (T0-T3).Serum Cav-1 is more sensitive (96.2%) than that in urine (86.1%) although urine Cav-1 is more specific(94.3%). Conclusion: Exosomal Cav-1 expression could be used as a crucialnon-invasive biomarker in diagnosis and prognosis of bladder cancer in serum and urine.
IMAGE SENTIMENTAL ANALYSIS: AN OVERVIEW
Authors:   Department of Computer Science and Engineering, East West Institute of Technology, Magadi Main Road, Anjana Nagar, Bangalore, Karnataka, 560091, India., Sunil Kumar V. , C.R Vedashree ,   Department of Computer Science and Engineering, East West Institute of Technology, Magadi Main Road, Anjana Nagar, Bangalore, Karnataka, 560091, India., S. Sowmyashree ,   Department of Computer Science and Engineering, East West Institute of Technology, Magadi Main Road, Anjana Nagar, Bangalore, Karnataka, 560091, India.
Published: April 2022

Visual content, such as photographs and video, contains not only objects, locations, and events, but also emotional and sentimental clues. On social networking sites, images are the simplest way for people to communicate their emotions. Images and videos are increasingly being used by social media users to express their ideas and share their experiences. Sentiment analysis of such large-scale visual content can aid in better extracting user sentiments toward events or themes, such as those in image tweets, so that sentiment prediction from visual content can be used in conjunction with sentiment analysis of written content. Despite the fact that this topic is relatively new, a wide range of strategies for various data sources and challenges have been created, resulting in a substantial body of study. This paper introduces the area of Image Sentiment Analysis and examines the issues that it raises. A description of new obstacles is also included, as well as an assessment of progress toward more sophisticated systems and related practical applications, as well as a summary of the studys findings.
AN ASSESSMENT OF ELASTOSIS IN FIBROCYSTIC BREAST DISEASE AND INVASIVE BREAST CARCINOMA
Authors: Duraisamy R. , Maheswari S. , Suganya U.
Published: April 2022

Fibrocystic disease is one of the most common benign condition of the breast. Its incidence is significantly increased by nulliparity, late age of first birth, and late menopause, factors also associated with increased risk for breast carcinoma risk. Breast cancer is the second leading cause of cancer related deaths in females all over the world. Materials and methods : In the present study 30 cases of fibrocystic disease and carcinoma breast were taken and studied for the presence and grade of elastosis using Hematoxylin Eosin staining and Verhoeff Van Gieson staining. Results : The association between ductal epithelial hyperplasia and grade of elastosis in the Fibrocystic breast disease increased with the severity of the epithelial hyperplasia, but was not statistically significant . The correlation between histological grade of invasive ductal carcinoma-NOS type and grade of elastosis was statistically insignificant, but may be used as predictive marker of response to endocrine therapy.
IDEAL LIFESTYLE OF POST COVID PATIENTS
Authors:   PG Scholar, Deptt. Of Samhita Siddhant, PT. Khushilal Sharma Govt. Auto. College and Hospital, Bhopal., Khare Abha , Kumar Jain Salil ,   Lecturer M.D, Ph.D. Deptt. Of Samhita Siddhant, PT. Khushilal Sharma Govt. Auto. College and Hospital, Bhopal., Gupta Nikita ,   PG Scholar, Deptt. Of Samhita Siddhant, PT. Khushilal Sharma Govt. Auto. College and Hospital, Bhopal.
Published: April 2022

The article reviews basic prophylactic and curative approaches mentioned in Ayurveda classics. As the current scenario is worst across the nation due to severe covid outrage it becomes merely important to explore and tell society about the way of living through the science of life and not only a curative science, Ayurveda, several basic principles are mentioned in it which should be followed by everyone like Dicharya, Ritucharya, Aahar,vihar , Rasayana etc, these are the tools shielding protection from such infection and its post affect to a great extent. Ayurveda focuses mainly on understanding the pathogenesis through basic principles such as tri-sutra and treat it according .This can frame a basic treatment approach also against this devil covid-19 which can be useful in treating mild cases as well as Post covid cases easily and to a great extent.
THE ROLE OF JUDICIAL ACCOUNTING AND ITS IMPACT IN REDUCING THE PHENOMENON OF MONEY LAUNDERING
Authors:   Faculty of College of Administrative Technology, Southern Technical University, Iraq., Jerri Mohsin Hayder , Yousef Bani Hani Laith ,   Researcher: Osmania University- Business Management and Commerce College, India., Aldeen Kassem Al-Alawnh Noor ,   Ph.D. Student Putra business school, University Putra Malaysia, Malaysia., Mohammed Al-Ali Huthefh ,   Researcher: Faculty of Economics and Administrative Sciences,Yarmouk University, Jordan., Hamad Al-Khalaileh Ashraf ,   Ph.D. Student Putra business school, University Putra Malaysia, Malaysia., Qasem Qased Wassan ,   Iraqi Ministry of oil / Basra Oil Company.
Published: April 2022

Purpose: The research problem in the presence of money laundering in private banks reflects negatively on economic activities. The purpose of this study is to investigate the dimensions of forensic accounting (analysis of sources of use and funds, forensic accounting path, temporal analysis process, cash flow tracking schedule for various activities, analysis of the link between people and their relationship to financial data, follow-up of suspicious financial transactions) and its impact on reducing the phenomenon of money laundering. The study methodology used quantitative analysis of data related to private banks in the province of Basra under study, which is(7) private banks. The study sample included all Finances Accountants in these banks, numbering (150) individuals, and the data was processed through statistical methods using the program (SPSS). Results: Statistics, statistical analysis results, statistical tracking table, persons and their relationship to financial bytes, follow-up of financial transactions, and the dependent variable money laundering.
ROLE OF FIBEROPTIC BRONCHOSCOPY IN SPUTUM NEGATIVE PATIENTS
Authors: Sharma Rachit , Prasad Rajendra , Kacker Rishabh , Raza Tasleem , Srivastava Amit
Published: April 2022

Background:Tuberculosis continues to be the one of the most common global health problems for more than a century. Fiberoptic bronchoscopy may be very useful in diagnosing cases who have no sputum or whose sputum smear is negative for Acid-Fast bacilli. The current study aims to assess the role of fiberoptic bronchoscopy in sputum smear negative under NTEP and radiologically suspected cases of pulmonary tuberculosis. Methods:This study was conducted in our institution in clinico-radiological suspected cases of pulmonary tuberculosis patients in whom two sputum smear for acid fast bacilli by Zeihl-Neelsen stain under NTEP was negative. Fiberoptic bronchoscopy was performed in all these patients and samples taken were sent for investigations. Result:Fiberoptic bronchoscopy was performed in a total of 110 patients of presumptive pulmonary tuberculosis whose sputum for AFB smear was negative. Out of 110 patients, 65.45% were male and 35.55% were female. Fever was the most common presenting symptom and was found in 93.64% patients. Radiographically, pulmonary consolidation was bilateral in 63.34% cases while was unilateral in 36.36% cases. The total yield of FOB came out to be 59.1% in which BAL for AFB smear was positive in 43.63% cases, BAL for Mtb culture was positive in 54.54% cases while, BAL for PCR was found to be positive in 59.09% cases. Conclusion:Fiberoptic bronchoscopy may be useful in rapid diagnosis and early treatment initiation thereby, reducing the risk of transmission of the disease. The findings of the study indicated that fiberoptic bronchoscopy is a useful method to diagnose the cases of sputum negative radiologically suspected symptomatic patients of tuberculosis.