bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on Postfrac Softening Mechanism of Deep Marine Shale Reservoir in South Sichuan Basin
Authors: Xiaojin Zhou , Yonggang Duan , XiaoPing He , Bo Zeng , Yi Song
Published: January 2023

Shale softening is an important factor affecting fracturing effect. Through the Brinell hardness measurement of deep marine shale reservoir in South Sichuan Basin, the softening mechanism of shale after fracturing is explored. Through laboratory test, the key parameters of shale Brinell hardness measurement are proposed as follows: indenter size 5 mm, loading force 613 N, 1226 N, and 2452 N, and holding

time
>
10
 
s

. It is determined that the content of quartz and clay is the main factor controlling the Brinell hardness of dry shale samples, and the mineral composition, microstructure, and fluid system jointly affect the shale softening process. The results show that (1) the marine shale in the South Sichuan Basin softens obviously, with the Brinell hardness decrease by more than 50%, (2) microfracture propagation and clay hydration expansion accelerate the shale softening process, leading to the decreased porosity and the increased number of micro-pores, and (3) the softening process is different between middeep shale and deep shale, with the latter characterized by high initial hardness and low softening rate.
Effect of Load on Tribological Characteristics of H-AlSi24Cu3.8Mg0.8 Alloy Under Dry, Lubricated, and Coated (a–c: H) Sliding Conditions
Authors: Naik M. Peeru , Padal K. T. Balaram , Avvari Muralidhar
Published: January 2023

This paper presents the new route of fabrication of H-AlSi24Cu3.8Mg0.8 alloy, assisted by ultrasonic vibration stirring and squeeze casting (UVSS). The primary objective of this investigation is to establish the tribological characteristics of the prepared aluminium alloy under dry, lubricated, and coated sliding conditions. Oil SAE20W40 is used as a lubricant and diamond-like carbon (DLC) is used as a coating. Under a load between 20 N and 50 N, a linear reciprocating tribometer (LRT) with a ball-on-plate geometry is used. Alloy was examined using an optical microscope, scanning electron microscope with EDS, 3D surface profilometer, Brinell hardness tester, and tensometer for pre- and postcharacterization of alloy, surface, and worn tracks. The DLC-coated condition yields the lowest wear and coefficient of friction.
Sebaceous Carcinoma of the Penis: A Rare, Dangerous Clinical Entity and the Importance of Immunohistochemistry in Diagnosis
Authors: Sahni Vikram , Cassarino David S.
Published: January 2023

We report a very rare case of pathologically confirmed sebaceous carcinoma of the glans penis with multiple areas of lymphovascular and perineural invasion and multiple lymph node metastases. The importance of immunohistochemical staining in diagnosis is also reviewed.
Editorial
Authors: Franco Ruiz Rafael
Published: January 2023

Estamos frente a un renacimiento, el de la revista de divulgación SUMMA DE ARITHMETICA emitida como medio de información del programa de Magister en Contabilidad y Auditoría de la Facultad Economía y Administración de la Universidad de Santiago de Chile, y que hoy renace para dar espacio también al programa de pregrado de Contador Público y Auditor del Departamento de Contabilidad y Auditoría, y a otros noveles autores y autoras (seguir leyendo)
Progression of Recurrent Pancreatitis to Chronic Pancreatitis within 3 Years due to SPINK1 Mutation IVS3+2T>C
Authors: Horitani Susumu , Tsujimae Masahiro , Sakai Arata , Masuda Atsuhiro , Nagao Kae , Kohashi Shinya , Inomata Noriko , Uemura Hisahiro , Masuda Shigeto , Gonda Masanori , Abe Shohei , Yamakawa Kohei , Ashina Shigeto , Yamada Yasutaka , Tanaka Takeshi , Nakano Ryota , Kobayashi Takashi , Shiomi Hideyuki , Kodama Yuzo
Published: January 2023

When the etiology of pancreatitis cannot be determined despite sufficient investigation, recurrence and progression to chronic pancreatitis often involve genetic mutations. Herein, we describe a case of recurrent pancreatitis with the IVS3+2T>C mutation in the serine protease inhibitor Kazal type 1 (SPINK1) gene that progressed to chronic pancreatitis in only 3 years. A 35-year-old man was referred to our hospital, where he was diagnosed with mild pancreatitis and was treated conservatively. However, the patient experienced recurrent episodes of pancreatitis, which progressed to become chronic pancreatitis with a pancreatic calcification 1 year later. After 3 years, the patient developed pancreatic duct stenosis and required a pancreatic duct stent placement. Regarding the cause of chronic pancreatitis, alcohol abuse was ruled out based on history taking. Considering the course of treatment, autoimmune pancreatitis and obstructive pancreatitis, such as pancreatic divisum, were also ruled out. Finally, a germline genetic test was performed to determine the etiology of pancreatitis, which revealed the IVS3+2T>C mutation in SPINK1. This case shows the importance of genetic testing in patients with idiopathic pancreatitis to determine their etiology and is a rare incident that can report the progression of the disease from acute to chronic pancreatitis.
Comparison of Chorioretinal Parameters in Diabetic Retinopathy with or without Pan-Retinal Photocoagulation Using Ultrawide-Field Swept-Source Optical Coherence Tomography Angiography
Authors: Li Fang , Liu Lun , Rao Cai-hua , Gao Jian
Published: January 2023

Introduction: To compare structural and vascular parameters in the central and peripheral retina and choroid of eyes diagnosed with severe nonproliferative diabetic retinopathy (NPDR) or proliferative diabetic retinopathy (PDR) with or without pan-retinal photocoagulation (PRP) using ultrawide-field swept-source optical coherence tomography angiography (SS-OCTA). Methods: All participants underwent SS-OCTA imaging centered on the fovea. Retinal and choroidal thickness, vessel density of the superficial capillary plexus (SCP), deep capillary plexus (DCP), choroidal capillary plexus (CCP), and choroidal Sattler’s and Haller’s layer (CSHL) were analyzed in nine grids using built-in angiography analysis software. Results: A total of 82 eyes from 82 participants were enrolled in this study, including 40 eyes diagnosed with severe NPDR or PDR without PRP and 42 eyes with PRP. Retinal thickness in part grids was higher in eyes with PRP than in eyes without PRP. Vessel density of the SCP in nasal-superior (p = 0.003) grid was lower in eyes with PRP than in eyes without PRP. No significant difference was found in the vessel density of the DCP between the two groups (all p > 0.05). The choroidal thickness and vessel density of the CSHL of all grids were significantly lower in eyes with PRP than in eyes without PRP (all p < 0.05). A statistically significant decrease in vessel density in CCP was found in the superior (p = 0.043), nasal-superior grid (p = 0.003), macular grid (p < 0.001), and optic disc grid (p = 0.001) of eyes with PRP, compared to eyes without PRP. A significant decrease in the vessel density of CSHL was observed in all grids of PDR eyes with PRP compared to PDR eyes without PRP (all p < 0.05). Significant decrease in choroidal thickness was observed in most grids of PDR eyes with PRP, except for macular grid (p = 0.090) and optic disc grid (p = 0.057). Conclusion: Structural and vascular parameters in the central and peripheral retinal and choroidal layers in eyes diagnosed with severe NPDR or PDR with or without PRP could be quantified using a ultrawide-field SS-OCTA. Eyes with PRP showed a significant decrease in choroidal thickness and vessel density of CCP and CSHL, compared with eyes without PRP. This trend was more obvious in eyes with PDR.
Unravelling the stomach contents of fish and crab species from Cananéia, São Paulo: Are they eating plastic?
Authors: Gonçalves Geslaine Rafaela Lemos , Sousa Aline Nonato , Wolf Milena Regina , Soares Isabel Matos , Castilho Antonio Leão
Published: January 2023

Plastic pollution represents a threat to marine ecosystems and has therefore been gaining space in the realm of public interest. In this study, we investigated the ingestion of food and non-food items (i.e., plastic particles) by fish and crabs. These animals are commonly collected by trawling with a double-ring net along the coast of Cananéia, state of São Paulo, Brazil; some of them are consumed as food by the local population. Fish and crab stomachs were removed and dissected, and their contents were examined under a stereoscopic microscope with an image-capturing system. The presence or absence of plastic was also registered. We examined 139 specimens of 16 fish species and 143 specimens of four crab species. The most frequent food items found in fish were unidentified food, followed by crustaceans, molluscs, polychaetes, and other fish; in crabs, the items were unidentified food, followed by crustaceans, molluscs and fish. Plastic particles were found in all fish species, representing 47.5% of the individuals analysed. In crabs, the incidence of plastic was lower, occurring in only two species (5% in Callinectes danae and 3% in C. ornatus). Only four fish species analysed had previous records of plastic ingestion in the scientific literature. The high incidence of microplastics in our study is worrying because they negatively affect the animals’ lives and can be transferred through the tropic web to top predators, including humans, through the ingestion of contaminated animals.
Behavior and nest architecture of the bee Caenohalictus alexandrei (Hymenoptera, Halictinae)
Authors: Celis Cindy Julieth , Aguilar-Benavides Marlene Lucia , Cure José Ricardo
Published: January 2023

In Colombia, few studies have focused on nest architecture, behavior or sociality of wild bees. This study provides basic information on the nests of Caenohalictus alexandrei and presents behavioral observations outside the nests, derived from direct field observation of 40 nests and 39 male sleeping cavities in two localities of the Savanna of Bogota, Colombia. We recognized four different behavioral activities carried out by adult females: foraging, guarding, opening and closing of the entrance of the nest, with foraging being the most frequent of them. The observed activities were carried out more frequently from 9:00 h to 13:00 h. Males were observed performing two behavioral activities in the sleeping cavities: staying at the cavity entrance and cavity departure. A third male activity, corporal grooming, was carried out outside the sleeping cavity. Our field observations indicate that more than one adult female was active simultaneously performing the different tasks within a single nest. The nests presented different levels of complexity in their architecture, depending on the age of the nest and number of adult females in them. Descriptions of the nesting sites and behavioral activities as well as illustrations of the nest architecture are provided.
A new synonymy in Anisopodus White (Coleoptera, Cerambycidae, Lamiinae, Acanthocinini)
Authors: Santos-Silva Antonio , Nascimento Francisco Eriberto de Lima , Mejlon Hans
Published: January 2023

Anisopodus subarmatus Melzer, 1931 is synonymized with A. jaculus (Gyllenhal, 1817) and a discussion about the type-locality of the latter is provided. Photographs of the lectotype of the former and of the holotype of the latter are provided.
Creagrura Townes, 1971 and Ptilobaptus Townes, 1971 (Hymenoptera: Ichneumonidae) from Brazilian Amazon: new records and comments about morphological variation
Authors: Lima Karoline Gomes , Nascimento Alexsandra Cordeiro , Fernandes Daniell Rodrigo Rodrigues
Published: January 2023

Creagrura nigripes Townes, 1971 is recorded for the first time for the Brazilian Amazon, in the states of Acre, Amapá, Amazonas, Pará and Roraima. Ptilobaptus cinctus Townes, 1971 is registered for the first time in Brazil. Additionally, diagnosis, digital images, distribution maps, as well as comments on intraspecific morphological variations in the species are provided.
How Was It for You? Qualitative Observational Methods to Assess the Experiential Value of Two Immersive Augmented Reality Stories: A Case Study
Authors: Jefferies Liam Noah
Published: January 2023

Immersive storytelling using augmented reality is a well-established and widely researched practice with a growing body of literature spanning the last two decades in which researchers from various fields apply this technology to cultural spaces. Despite this, the methodologies used to assess the efficacy of these applications—even defined measures of what constitutes success—remain disparate and often aligned to the field from which they originated—these being HCI, Visitor Studies, and New Media Art. The form these interventions take and the modes of storytelling they employ is steadily diversifying alongside theories and definitions of what constitutes a story. In this context of divergence, a reimagination of the methods researchers use to evaluate and assess their projects would be useful to ensure the nuances of the engaged experience and the value it offers to audiences can be captured. This paper proposes a methodological approach, seeking to enable the evaluation of experiential value. This methodological approach is then applied to two augmented reality immersive stories, created by the author, the first an educational, linear narrative and the second a nonlinear narrative artwork. Using primarily qualitative narrative observation, participant interactions with both projects are explored, testing the application of experiential value as a measure and interrogating the chosen methodology's ability to capture it.
Nomenclator botanicus of Fagaceae in Latin America
Authors: BARTHOLOMEW BRUCE , ALMEDA FRANK
Published: January 2023

Latin America is one of the centers of Fagaceae distribution, at least for the genus Quercus. There are a total of 145 Fagaceae species native to Latin America of which 143 are Quercus, with the other two being one species of Fagus and one species of Trigonobalanus. Within Quercus, 32 species that occur in Latin America extend into the United States of America and one, Q. muehlenbergii, ranges as far north as Canada. By far the main center for Quercus in Latin America is Mexico (137 species of which 83 are endemic). The number of species rapidly falls off in Central America, and only one species (Q. humboldtii) occurs in South America (Colombia). In this nomenclator we include one new name (nom. nov.) for a recognized species that was a later homonym when published. We also lectotypify 171 names, and neotypify one species.
Enhancer remodeling drives MLL oncogene-dependent transcriptional dysregulation in leukemia stem cells
Authors: Pan Feng , Iwasaki Masayuki , Wu Wenqi , Jiang Yanan , Yang Xin , Zhu Li , Zhao Zhigang , Cleary Michael L.
Published: January 2023

Acute myeloid leukemia (AML) with mixed-lineage leukemia (MLL) gene rearrangement (MLLr) comprises a cellular hierarchy in which a subpopulation of cells serves as functional leukemia stem cells (LSCs). They are maintained by a unique gene expression program and chromatin states, which are thought to reflect the actions of enhancers. Here, we delineate the active enhancer landscape and observe pervasive enhancer malfunction in LSCs. Reconstruction of regulatory networks revealed a master set of hematopoietic transcription factors. We show that EP300 is an essential transcriptional coregulator for maintaining LSC oncogenic potential because it controls essential gene expression through modulation of H3K27 acetylation and assessments of transcription factor dependencies. Moreover, the EP300 inhibitor A-485 affects LSC growth by targeting enhancer activity via histone acetyltransferase domain inhibition. Together, these data implicate a perturbed MLLr-specific enhancer accessibility landscape, suggesting the possibility for disruption of the LSC enhancer regulatory axis as a promising therapeutic strategy in AML.
Deciphering evolutionary dynamics of WRKY genes in Arachis species
Authors: Chen Mingwei , Li Meiran , Zhao Longgang , Song Hui
Published: January 2023

Background

Cultivated peanut (Arachis hypogaea), a progeny of the cross between A. duranensis and A. ipaensis, is an important oil and protein crop from South America. To date, at least six Arachis genomes have been sequenced. WRKY transcription factors (TFs) play crucial roles in plant growth, development, and response to abiotic and biotic stresses. WRKY TFs have been identified in A. duranensis, A. ipaensis, and A. hypogaea cv. Tifrunner; however, variations in their number and evolutionary patterns across various Arachis spp. remain unclear.

Results

WRKY TFs were identified and compared across different Arachis species, including A. duranensis, A. ipaensis, A. monticola, A. hypogaea cultivars (cv.) Fuhuasheng, A. hypogaea cv. Shitouqi, and A. hypogaea cv. Tifrunner. The results showed that the WRKY TFs underwent dynamic equilibrium between diploid and tetraploid peanut species, characterized by the loss of old WRKY TFs and retention of the new ones. Notably, cultivated peanuts inherited more conserved WRKY orthologs from wild tetraploid peanuts than their wild diploid donors. Analysis of the W-box elements and protein–protein interactions revealed that different domestication processes affected WRKY evolution across cultivated peanut varieties. WRKY TFs of A. hypogaea cv. Fuhuasheng and Shitouqi exhibited a similar domestication process, while those of cv. Tifrunner of the same species underwent a different domestication process based on protein–protein interaction analysis.

Conclusions

This study provides new insights into the evolution of WRKY TFs in Arachis spp.
Towards full clinical trial registration and results publication: longitudinal meta-research study in Northwestern and Central Switzerland
Authors: Klatte Katharina , Sluka Constantin , Gloy Viktoria , Heravi Ala Taji , Schönenberger Christof , Jones Nienke , Brunnschweiler Elena , Pauli-Magnus Christiane , Briel Matthias
Published: January 2023

Objective

The registration of clinical trials is required by law in Switzerland. We investigated (1) the proportion of registered and prospectively registered clinical trials, (2) the availability of results for ethically approved trial protocols, (3) factors associated with increased registration, and (4) reasons for non-registration.

Design and setting

We included all clinical trials with mandatory prospective registration, which were approved by the ethics committee of Northwestern and Central Switzerland between January 1, 2016, and December 31, 2020.

Methods

We extracted relevant trial characteristics from the Swiss Business Administration System for Ethics Committees and systematically searched the International Clinical Trials Registry Platform and primary trial registries for corresponding registry entries. We used multivariable logistic regression to examine the association between trial characteristics and registration. We qualitatively assessed reasons for non-registration of trials through an email questionnaire for trial investigators.

Results

Of 473 included clinical trials, 432 (91%) were registered at all and 326 (69%) were prospectively registered. While the percentages of registration and prospective registration of investigator-sponsored trials increased from 85 to 93% and from 59 to 70% over 5 years, respectively, industry-sponsored trials consistently remained at a high level of prospective registration (92 to 100%). Trials with multiple centres, higher risk category, or methodological support from the local clinical trials unit were independently associated with increased registration rates. Of 103 clinical trials completed before August 2020, results were available for 70% of industry-sponsored trials and 45% of investigator-sponsored trials as peer-reviewed journal publications or in trial registries. Most common reasons for non-registration provided by investigators were lack of time or resources (53%), lack of knowledge (22%), and lack of reminders by the ethics committee (36%).

Conclusions

In Northwestern and Central Switzerland about 10% of clinical trials remained unregistered despite the obligation by law. More support for investigators and stricter enforcement by regulators are needed to improve the transparency of investigator-sponsored trials in particular.
Nursing home adjustment in China: mediating and moderating effects
Authors: Yong Binbin , Zhang Yanyan , Xiao Huimin
Published: January 2023

Purpose

Adjusting to nursing homes contributes to successful aging in older adults. However, the effect of stress on psychological adjustment in nursing home residents is unclear. Therefore, this study aimed to explore the relationship between stress and psychological adjustment among nursing home residents.

Methods

This was a cross-sectional, multicenter survey. A total of 386 residents from 11 nursing homes were included in the study. Bootstrapping with resampling strategies was used to examine multiple mediators and the moderator effect. This research conforms with the STROBE checklist.

Results

Path analysis demonstrated that stress directly negatively predicted the psychological adjustment of nursing home residents. It also indirectly predicted psychological adjustment through the mediating role of learned resourcefulness and self-efficacy, as well as the chain mediating role of both. Social support played a moderating role in the path of stress to learned resourcefulness.

Conclusion

This study revealed the mechanism underlying the effect of stress on psychological adjustment in nursing home residents without cognitive impairment and walking difficulties. It further suggests that health providers could enhance older adults' learned resourcefulness, self-efficacy, and social support to assist them in adjusting to nursing home life.
Baseline characteristics and treatment response predictive of nAMD outcomes with ranibizumab therapy in treatment-naive patients: the RACER subgroup analysis
Authors: Tsai Ching-Yao , Wu Chien-Liang , Cheng Cheng-Kuo , Shen Yun-Dun , Wu Wen-Chuan , Wu Pei-Chang , Tsai Arslan , Chen Jiann-Torng
Published: January 2023

Background

The Ranibizumab AMD Clinical Efficacy Study (RACER) conducted in treatment-naive adult Taiwanese patients with neovascular age-related macular degeneration (nAMD) suggested the importance of early and intensive dosing of ranibizumab for optimal treatment outcomes. This subgroup analysis aims to provide clinical information on treatment response that can potentially guide on maintaining the treatment or switching anti-VEGF agents in the real-world setting.

Methods

Visual acuity (VA) and central retinal thickness (CRT) were assessed in the RACER subgroup population. Subgroup analysis sets were categorised based on: (1) baseline best-corrected VA (BCVA; ≤ 48 and > 48 letters); (2) baseline CRT (≤ 325 or > 325 μm); and (3) treatment response after three monthly initial injections: < or ≥ 5-letter gain in BCVA and reduction of < or ≥ 50 μm in CRT.

Results

Patient age, sex, nAMD duration and number of ranibizumab injections did not differ significantly between the treatment subgroups. Poor baseline BCVA (≤ 48 letters) and baseline CRT severity (> 325 µm) were predictors of maximum BCVA gains (9.6 ± 12.9 letters [95%CI: 6.3 to 12.9] and 5.1 ± 18.3 letters [95%CI: − 0.5 to 10.8] at Months 3 and 12, respectively) and better CRT reductions (− 127.6 ± 104.2 µm and − 104.2 ± 107.4 µm at Months 3 and 12, respectively; both P < 0.001). For the subgroup showing favourable treatment improvement with BCVA gains ≥ 5 letters after three monthly initial injections, 75.6% of patients maintained follow-up at Month 12 with a mean of 6.5 ± 14.3 letter gains (95% CI: 1.2 to 11.7). The BCVA gains < 5-letter subgroup nevertheless had stable BCVA (0.4 ± 12.1 letter gains) and CRT (− 41.9 ± 61.2 µm) at Month 12, respectively. In the subgroup with ≥ 50 µm CRT reduction after three monthly initial injections, there are significantly higher BCVA improvements vs. the < 50 µm CRT reduction subgroup at Month 3 (5.0 ± 8.6 letter gains vs. 1.5 ± 11.6 letter gains, respectively; intergroup P = 0.005).

Conclusion

Lower baseline BCVA and higher baseline CRT were associated with BCVA gains and CRT reductions throughout the 12-month study period. Early CRT improvements after three monthly initial injections were associated with BCVA gains as early as Month 3.
The influence of disordered eating and social media’s portrayals of pregnancy on young women’s attitudes toward pregnancy
Authors: Gibson A. Hope , Zaikman Yuliana
Published: January 2023

Background

Given the heightened emphasis on physical appearance and the prevalence of social media in young women, they are particularly vulnerable to experiencing negative body image and disordered eating. Therefore, modified social media portrayals of pregnancy could cause young women to have negative attitudes toward a potential pregnancy and subsequently not properly utilize care and resources. The present study examined the influence of disordered eating and modified portrayals of pregnancy on young women’s attitudes toward a potential pregnancy and various feelings associated with pregnancy.

Methods

The sample consisted of 154 women aged 18–30, who were given the Eating-Attitudes Test-26, randomly shown either modified or unmodified social media portrayals of pregnancy, then given the Attitudes Toward Potential Pregnancy Scale and the Gestational Weight Gain Psychosocial Risk Assessment Tool.

Results

A series of hierarchal regressions revealed that there were no significant main effects or interactions for young women’s attitudes toward potential pregnancy. However, women who viewed modified portrayals of pregnancy had higher self-efficacy, and women with higher levels of disordered eating had lower self-efficacy, more positive attitudes toward gestational weight gain, and lower current body image satisfaction.

Conclusions

These results highlight the myriad of different attitudes that young women have toward a potential pregnancy and how these attitudes are influenced by disordered eating and social media. Our findings can be used for educating caregivers and implementing intervention strategies for women.
Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds
Authors: Soliman Hesham S. M. , Korany Eslam M. , El-Sayed Elsayed K. , Aboelyazed Ahmed M. , Ibrahim Haitham A.
Published: January 2023

Background

Cadmium is an environmentally toxic metal that has deleterious effects on both animals and humans due to its accumulation in different body tissues. Physalis peruviana L. fruit and calyx contain many active constituents which are used traditionally for their different biological activities. Based on the traditional uses of P. peruviana L. calyx, we aimed to evaluate the nephroprotective effect of their 80% aqueous methanol extract (AME) and n-butanol fraction (Bu.F.) against cadmium chloride-induced nephrotoxicity in rats and to correlate this activity with phytoconstituents isolated using molecular docking studies.

Methods

The n-butanol fraction of P. peruviana L. calyx was fractionated using various chromatographic techniques and the isolated compounds were identified based on their chemical and spectroscopic data. The nephroprotective activity was assessed using cadmium chloride-induced nephrotoxicity in the rat model, by measuring some important parameters such as body weight, kidney weight, serum urea, and creatinine levels, oxidative stress markers, inflammatory markers, and histopathological examinations of kidney tissue. Molecular docking studies of the isolated compounds were performed.

Results

Three withanolides named 4 β-hydroxywithanolide E (1), Physalin B (2) and 3α, 14β-dihydroxywithaphysalin N (3) were isolated and identified from the n-butanol fraction of P. peruviana L calyx extract. The extract and its butanol fraction significantly improved the serum kidney function markers and tissue oxidative status including malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT). Additionally, the extracts significantly decreased the levels of tumor necrosis factor-alpha (TNF-α) and nuclear factor kappaB (NF-κβ). Moreover, the histological changes were ameliorated by the extracts. The molecular docking study showed that the isolated compounds displayed a remarkable inhibitory activity against IκB kinase.

Conclusion

The AME and its butanol fraction of P. peruviana L calyx showed potential nephroprotective activity against cadmium chloride-induced nephrotoxicity which is correlated at least in part to its considerable withanolides content.
Genomic surveillance of SARS-CoV-2 in COVID-19 vaccinated healthcare workers in Lebanon
Authors: AlKalamouni Habib , Abou Hassan Farouk F. , Bou Hamdan Mirna , Page Andrew J. , Lott Martin , Matthews Michaela , Ghosn Nada , Rady Alissar , Mahfouz Rami , Araj George F. , Dbaibo Ghassan , Zaraket Hassan , Melhem Nada M. , Matar Ghassan M.
Published: January 2023

Background

The emergence of SARS-CoV-2 variants including the Delta and Omicron along with waning of vaccine-induced immunity over time contributed to increased rates of breakthrough infection specifically among healthcare workers (HCWs). SARS-CoV-2 genomic surveillance is an important tool for timely detection and characterization of circulating variants as well as monitoring the emergence of new strains. Our study is the first national SARS-CoV-2 genomic surveillance among HCWs in Lebanon.

Methods

We collected 250 nasopharyngeal swabs from HCWs across Lebanon between December 2021 and January 2022. Data on the date of positive PCR, vaccination status, specific occupation, and hospitalization status of participants were collected. Extracted viral RNA from nasopharyngeal swabs was converted to cDNA, library prepped using the coronaHIT method, followed by whole genome sequencing on the Illumina NextSeq 500 platform.

Results

A total of 133 (57.1%) samples belonging to the Omicron (BA.1.1) sub-lineage were identified, as well as 44 (18.9%) samples belonging to the BA.1 sub-lineage, 28 (12%) belonging to the BA.2 sub-lineage, and only 15 (6.6%) samples belonging to the Delta variant sub-lineage B.1.617.2. These results show that Lebanon followed the global trend in terms of circulating SARS-CoV-2 variants with Delta rapidly replaced by the Omicron variant.

Conclusion

This study underscores the importance of continuous genomic surveillance programs in Lebanon for the timely detection and characterization of circulating variants. The latter is critical to guide public health policy making and to timely implement public health interventions.
The impact of diagnostic delay on survival in alpha-1-antitrypsin deficiency: results from the Austrian Alpha-1 Lung Registry
Authors: Meischl Tobias , Schmid-Scherzer Karin , Vafai-Tabrizi Florian , Wurzinger Gert , Traunmüller-Wurm Eva , Kutics Kristina , Rauter Markus , Grabcanovic-Musija Fikreta , Müller Simona , Kaufmann Norbert , Löffler-Ragg Judith , Valipour Arschang , Funk Georg-Christian
Published: January 2023

Background

Alpha-1-antitrypsin (AAT) deficiency (AATD) is a genetic disorder that can manifest as lung disease. A delay between onset of symptoms and diagnosis of AATD is common and associated with worse clinical status and more advanced disease stage but the influence on survival is unclear.

Objective

We aimed to investigate the impact of diagnostic delay on overall survival (OS) and transplant-free survival (TS) in AATD patients.

Methods

We analysed 268 AATD patients from the prospective multi-centre Austrian Alpha-1 Lung (AAL) Registry, employing descriptive statistics, Chi-square-test as well as univariable (Kaplan–Meier plots, log-rank test) and multivariable survival analysis (Cox regression).

Results

The predominant phenotype was Pi*ZZ (82.1%). At diagnosis, 90.2% had an AAT level below 0.6 g/L. At inclusion, 28.2% had never smoked, 68.0% had quit smoking and 3.8% continued to smoke. Lung disease was diagnosed in 98.5%, thereof most patients were diagnosed with emphysema (63.8%) and/or chronic obstructive pulmonary disease (44.0%). Median diagnostic delay was 5.3 years (inter-quartile range [IQR] 2.2–11.5 years). In multivariable analysis (n = 229), a longer diagnostic delay was significantly associated with worse OS (hazard ratio [HR] 1.61; 95% CI 1.09–2.38; p = 0.016) and TS (HR 1.43; 95% CI 1.08–1.89; p = 0.011), independent from age, smoking status, body mass index (BMI), forced expiratory volume in one second (FEV1) and long-term oxygen treatment. Furthermore, BMI, age and active smoking were significantly associated with worse OS as well as BMI, active smoking and FEV1 were with worse TS.

Conclusions

A delayed diagnosis was associated with significantly worse OS and TS. Screening should be improved and efforts to ensure early AATD diagnosis should be intensified.
Mannosylated-serum albumin nanoparticle imaging to monitor tumor-associated macrophages under anti-PD1 treatment
Authors: Gu Gyo Jeong , Chung Hyewon , Park Ji Yong , Yoo Ranji , Im Hyung-Jun , Choi Hongyoon , Lee Yun-Sang , Seok Seung Hyeok
Published: January 2023

Background

Immune checkpoint inhibitors such as anti-programmed cell death protein 1 (PD1) block tumor growth by reinvigorating the immune system; however, determining their efficacy only by the changes in tumor size may prove inaccurate. As the immune cells including macrophages in the tumor microenvironment (TME) are associated with the response to anti-PD1 therapy, tumor-associated macrophages (TAMs) imaging using nanoparticles can noninvasively provide the immune enrichment status of TME. Herein, the mannosylated-serum albumin (MSA) nanoparticle was labeled with radioactive isotope 68Ga to target the mannose receptors on macrophages for noninvasive monitoring of the TME according to anti-PD1 therapy.

Results

B16F10-Luc and MC38-Luc tumor-bearing mice were treated with anti-PD1, and the response to anti-PD1 was determined by the tumor volume. According to the flow cytometry, the responders to anti-PD1 showed an increased proportion of TAMs, as well as lymphocytes, and the most enriched immune cell population in the TME was also TAMs. For noninvasive imaging of TAMs as a surrogate of immune cell augmentation in the TME via anti-PD1, we acquired [68Ga] Ga-MSA positron emission tomography. According to the imaging study, an increased number of TAMs in responders at the early phase of anti-PD1 treatment was observed in both B16F10-Luc and MC38-Luc tumor-bearing mice models.

Conclusion

As representative immune cells in the TME, non-invasive imaging of TAMs using MSA nanoparticles can reflect the immune cell enrichment status in the TME closely associated with the response to anti-PD1. As non-invasive imaging using MSA nanoparticles, this approach shows a potential to monitor and evaluate anti-tumor response to immune checkpoint inhibitors.
Niche formation and function in developing tissue: studies from the Drosophila ovary
Authors: Jin Jian , Zhao Ting
Published: January 2023

Adult stem cells have a unique ability to self-renew and to generate differentiated daughter cells that are required in the body tissues. The identity of adult stem cells is maintained by extrinsic signals from other cell types, known as niche cells. Thus, the niche is required for appropriate tissue homeostasis. Niche is formed and recruits stem cells during tissue development; therefore, it is essential to establish niche cells and stem cells in proper numbers during development. A small niche may recruit too few stem cells and cause tissue degeneration, while a large niche may maintain too many stem cells and lead to tumorigenesis. Given that vertebrate tissues are not suitable for large-scale forward genetics studies, the Drosophila ovary stands out as an excellent model for studying how multiple niche cell types and germ cells (GCs) are coordinately regulated in vivo. Recent studies are beginning to reveal how various signaling molecules regulate niche formation and how niche cells non-autonomously influence GC number. In this review, we summarize the ovarian niche structure, the key signaling pathways for niche formation, and how niche cells generate extrinsic factors to control GC proliferation during ovarian development.
Megaprosthetic reconstruction of the distal femur with a short residual proximal femur following bone tumor resection: a systematic review
Authors: Tsukamoto Shinji , Mavrogenis Andreas F. , Masunaga Tomoya , Kido Akira , Honoki Kanya , Tanaka Yuu , Fujii Hiromasa , Tanaka Yasuhito , Errani Costantino
Published: January 2023

Background

To investigate the risk of postoperative function and complications associated with reconstruction methods in patients with short residual proximal femurs (< 12 cm) after resection of distal femoral bone tumors, we performed a systematic review of studies reporting postoperative function and complications in these patients.

Methods

Of the 236 studies identified by systematic searches using the Medline, Embase, and Cochrane Central Register of Controlled Trials databases, eight were included (none were randomized controlled trials). In these studies, 106 (68.4%), 12 (7.7%), and 37 (23.9%) patients underwent reconstruction with custom-made megaprostheses with extracortical plates or cross-pins, allograft prosthetic composite (APC), and Compress® compliant pre-stress (CPS) implants, respectively.

Results

Aseptic loosening occurred slightly more frequently in the APC group than in the other reconstruction methods (APC group, 21%; custom-made megaprosthesis group, 0–17%; CPS implant group, 14%). No differences were noted in the frequencies of implant breakage, fractures, or infections between the three reconstruction methods. Mechanical survival, where endpoint was set as implant removal for any reason, was 80% at seven years in the APC group, 70–77% at 10 years in the custom-made megaprosthesis group, and 68% at nine years in the CPS implant group. Therefore, there appeared to be no difference among the three reconstruction methods with respect to mechanical survival.

Conclusions

During megaprosthetic reconstruction of the distal femur with a short residual proximal femur after bone tumor resection, similar results were obtained using custom-made megaprostheses, APCs, and CPS implants.
Task scheduling in cloud environment: optimization, security prioritization and processor selection schemes
Authors: Hai Tao , Zhou Jincheng , Jawawi Dayang , Wang Dan , Oduah Uzoma , Biamba Cresantus , Jain Sanjiv Kumar
Published: January 2023

Cloud computing is an extremely important infrastructure used to perform tasks over processing units. Despite its numerous benefits, a cloud platform has several challenges preventing it from carrying out an efficient workflow submission. One of these is linked to task scheduling. An optimization problem related to this is the maximal determination of cloud computing scheduling criteria. Existing methods have been unable to find the quality of service (QoS) limits of users- like meeting the economic restrictions and reduction of the makespan. Of all these methods, the Heterogeneous Earliest Finish Time (HEFT) algorithm produces the maximum outcomes for scheduling tasks in a heterogeneous environment in a reduced time. Reviewed literature proves that HEFT is efficient in terms of execution time and quality of schedule. The HEFT algorithm makes use of average communication and computation costs as weights in the DAG. In some cases, however, the average cost of computation and selecting the first empty slot may not be enough for a good solution to be produced. In this paper, we propose different HEFT algorithm versions altered to produce improved results. In the first stage (rank generation), we execute several methodologies to calculate the ranks, and in the second stage, we alter how the empty slots are selected for the task scheduling. These alterations do not add any cost to the primary HEFT algorithm, and reduce the makespan of the virtual machines’ workflow submissions. Our findings suggest that the altered versions of the HEFT algorithm have a better performance than the basic HEFT algorithm regarding decreased schedule length of the workflow problems.
Effect of traditional herbal medicine, danggui-yukhwang-tang, on post-menopausal weight gain in ovariectomized high-fat diet rats
Authors: Jung Dong Ho , Yang Hyun , Hwang Joo Tae , Ko Byoung-Seob
Published: January 2023

The decrease in estrogen due to menopause leads to impaired lipid metabolism and is closely related to the increase in metabolic syndrome due to weight gain. Hormone replacement therapy is effective for menopause, but with an increased risk of side effects. Danggui-yukhwang-tang (DYT) is a traditional drug, comprising seven herbs, used to treat diseases like slight fever with sweating, blood-flow disorders, and neurasthenia. However, the effect on menopausal obesity has not been reported. This study aimed to investigate the inhibitory effect of DYT on weight gain in female rats fed a high-fat diet after ovariectomy. Adipocyte differentiation was effectively reduced by DYT in 3T3-L1 cells, and the mRNAs of PPARγ, C/EBPα, and FABP4, which are adipogenesis-related genes, were reduced. In the in vivo study, OVX and HFD elevated body weight; however, its induction significantly decreased in the DYT-treated groups. The serum lipid profile was also examined, and DYT treatment significantly decreased LDL-cholesterol, triglyceride, and total cholesterol levels compared with the OVX and OVX + HFD groups. DYT treatment effectively reduced the temperature(s) of the tail and body in the rats. The study demonstrates that DYT inhibits adipogenic differentiation, hypercholesterolemia, and weight gain in a post-menopausal rat model by regulating adipogenic markers (PPARγ, C/EBPα, FABP4) and the serum lipid profile in OVX + HFD rats.
Recommendations towards the establishment of best practice standards for handling and intracoelomic implantation of data-storage and telemetry tags in tropical tunas
Authors: Leroy Bruno , Scutt Phillips Joe , Potts Joanne , Brill Richard W. , Evans Karen , Forget Fabien , Holland Kim , Itano David , Muir Jeff , Pilling Graham , Nicol Simon
Published: January 2023

Archival (data-storage) and telemetry (acoustic and radio) tags are commonly used to provide data on the behavior and physiology of organisms, as well as data on their surrounding environment. For fishes, it is often advantageous to implant tags in the peritoneal cavity (i.e., intracoelomic implantation). The literature on best practices is limited for marine species, and near absent for tunas despite their regular application. We identify recommended practices using laparotomy in tropical tuna species following observations from thousands of tags implantations undertaken during implementation of several tagging programs across the Pacific. These recommended practices include descriptions of preferred tagging stations and equipment, fish selection, surgical procedures, and return of the fish to the wild. While these recommended practices were developed specifically for tropical tuna species, they are also likely applicable for other pelagic fishes. We present these guidelines to guide and promote the development of best practices for such procedures on pelagic species.
Influence of decomposition agent application and schedule in wheat straw return practice on soil quality and crop yield
Authors: Wang Xiaogang , Wang Qiongshan , Zhang Youchang , Zhang Jiaohai , Xia Songbo , Qin Hongde , Feng Changhui , Bie Shu
Published: January 2023

Straw return is a beneficial agricultural practice but mechanisms and information on microbial community alterations initiated by decomposed straw and promotion of soil nutritive content and conservation is still not adequately understood. Therefore, the study applied molecular, bioinformatics, chemical and plant yield analysis to determine the effect of wheat straw return and decomposing agent on soil, fungi and crop yield characteristics under wheat biomass return with and without the inclusion of the decomposition agent at varying times. We hypothesized no difference in fungal community diversity and composition, no changes in soil characteristics as well as no changes in cotton growth characteristics between three different conditions (i) no straw return, (ii) straw return under decomposition agent and (iii) straw return under no decomposition agent. It involved analysis of changes in fungal community diversity and composition, changes in soil characteristics as well as changes in cotton growth characteristics under the different treatments. The phyla Ascomycota and Basidiomycota were in relatively in highest abundance within the straw return under decomposition agent treatment than in both the straw return treatment and straw return under no decomposition agent treatment. The pathogenic genus Aspergillus as the most dominant under no straw return treatment, while genus Trechispora, Lulwaona and Dioszegia were most dominant under straw return under no decomposition agent treatment. Indeed, there was high fungal community dissimilarity between the three treatments. Additionally, there was increased rise in fungal diversity, soil nutrients and crop yield under the straw return within the shortest possible time, and the addition of decomposing agent further enhanced the high fungal species diversity.

Graphical Abstract
Contribution of natural food environments to nutritional intake and biomarker status: insights from the women of indigenous santhal communities of Jharkhand, India
Authors: Singh Archna , Dhasmana Ayushi , Bandhu Ashish , Kapoor Ridhima , Baalasubramanian Sivasankar , Ghosh-Jerath Suparna
Published: January 2023

Background

Many indigenous communities reside in biodiverse environments replete with natural food sources but show ​poor access and utilization.

Methods

To understand the links between indigenous food access, dietary intakes, and biomarkers, we conducted a cross-sectional study among women of the Santhal Community (n = 211) from 17 villages in the Godda district of Jharkhand, India. Survey methods included household surveys, dietary intake assessment (24 HDR) and micronutrient and inflammatory biomarkers' estimation.

Results

The diversity in access to foods from different natural sources expressed as Food access diversity index was low. This led to poor consumption and thus a low Minimum Dietary Diversity. The mean nutrient intake was less than the estimated average requirement for all nutrients. Women with higher dietary diversity scores had higher nutrient intakes. Thiamine and calcium intakes were significantly higher in women consuming indigenous foods than non-consumers. One-fourth of the women had elevated levels of inflammatory biomarkers. The prevalence of iron deficiency was approximately 70%. Vitamin A insufficiency (measured as retinol-binding protein) was observed in around 33.6% women, while 28.4% were deficient. Household access to natural food sources was associated with specific biomarkers. The access to kitchen garden (baari) was positively associated with retinol-binding protein levels and negatively with inflammatory biomarkers, while access to ponds was positively associated with ferritin levels.

Conclusion

The findings highlight the role of access to diverse natural foods resources, including indigenous foods, for improving nutrition security in indigenous communities. Nutrition and health programs promoting indigenous food sources should include the assessment of biomarkers for effective monitoring and surveillance.