bluuebunny/crossref_metadata_2025 huggingface dataset:
Multidrug Resistant Klebsiella pneumoniae ST101 Clone Survival Chain From Inpatients to Hospital Effluent After Chlorine Treatment
Authors: Popa Laura Ioana , Gheorghe Irina , Barbu Ilda Czobor , Surleac Marius , Paraschiv Simona , Măruţescu Luminiţa , Popa Marcela , Pîrcălăbioru Graţiela Grădişteanu , Talapan Daniela , Niţă Mihai , Streinu-Cercel Anca , Streinu-Cercel Adrian , Oţelea Dan , Chifiriuc Mariana Carmen
Published: January 2021

In this paper we describe the transmission of a multi-drug resistant Klebsiella pneumoniae ST101 clone from hospital to wastewater and its persistence after chlorine treatment. Water samples from influents and effluents of the sewage tank of an infectious diseases hospital and clinical strains collected from the intra-hospital infections, during a period of 10 days prior to wastewater sampling were analyzed. Antibiotic resistant K. pneumoniae strains from wastewaters were recovered on selective media. Based on antibiotic susceptibility profiles and PCR analyses of antibiotic resistance (AR) genetic background, as well as whole-genome sequencing (Illumina MiSeq) and subsequent bioinformatic analyses, 11 ST101 K. pneumoniae strains isolated from hospital wastewater influent, wastewater effluent and clinical sector were identified as clonally related. The SNP and core genome analyses pointed out that five strains were found to be closely related (with ≤18 SNPs and identical cgMLST profile). The strains belonging to this clone harbored multiple acquired AR genes [blaCTX–M–15, blaOXA–48, blaOXA–1, blaSHV–106, blaTEM–150, aac(3)-IIa, aac(6′)-Ib-cr, oqxA10, oqxB17, fosA, catB3, dfrA14, tet(D)] and chromosomal mutations involved in AR (ΔmgrB, ΔompK35, amino acid substitutions in GyrA Ser83Tyr, Asp87Asn, ParC Ser80Tyr). Twenty-nine virulence genes involved in iron acquisition, biofilm and pili formation, adherence, and the type six secretion system – T6SS-III were identified. Our study proves the transmission of MDR K. pneumoniae from hospital to the hospital effluent and its persistence after the chlorine treatment, raising the risk of surface water contamination and further dissemination to different components of the trophic chain, including humans.
Assessing the Effects of β-Triketone Herbicides on the Soil Bacterial and hppd Communities: A Lab-to-Field Experiment
Authors: Thiour-Mauprivez Clémence , Devers-Lamrani Marion , Bru David , Béguet Jérémie , Spor Aymé , Mounier Arnaud , Alletto Lionel , Calvayrac Christophe , Barthelmebs Lise , Martin-Laurent Fabrice
Published: January 2021

Maize cultivators often use β-triketone herbicides to prevent the growth of weeds in their fields. These herbicides target the 4-HPPD enzyme of dicotyledons. This enzyme, encoded by the hppd gene, is widespread among all living organisms including soil bacteria, which are considered as “non-target organisms” by the legislation. Within the framework of the pesticide registration process, the ecotoxicological impact of herbicides on soil microorganisms is solely based on carbon and nitrogen mineralization tests. In this study, we used more extensive approaches to assess with a lab-to-field experiment the risk of β-triketone on the abundance and the diversity of both total and hppd soil bacterial communities. Soil microcosms were exposed, under lab conditions, to 1× or 10× the recommended dose of sulcotrione or its commercial product, Decano®. Whatever the treatment applied, sulcotrione was fully dissipated from soil after 42 days post-treatment. The abundance and the diversity of both the total and the hppd bacterial communities were not affected by the herbicide treatments all along the experiment. Same measurements were led in real agronomical conditions, on three different fields located in the same area cropped with maize: one not exposed to any plant protection products, another one exposed to a series of plant protection products (PPPs) comprising mesotrione, and a last one exposed to different PPPs including mesotrione and tembotrione, two β-triketones. In this latter, the abundance of the hppd community varied over time. The diversity of the total and the hppd communities evolved over time independently from the treatment received. Only slight but significant transient effects on the abundance of the hppd community in one of the tested soil were observed. Our results showed that tested β-triketones have no visible impact toward both total and hppd soil bacteria communities.
Genome Mining and Characterization of Biosynthetic Gene Clusters in Two Cave Strains of Paenibacillus sp.
Authors: Lebedeva Jolanta , Jukneviciute Gabriele , Čepaitė Rimvydė , Vickackaite Vida , Pranckutė Raminta , Kuisiene Nomeda
Published: January 2021

The genome sequencing and mining of microorganisms from unexplored and extreme environments has become important in the process of identifying novel biosynthetic pathways. In the present study, the biosynthetic potential of Paenibacillus sp. strains 23TSA30-6 and 28ISP30-2 was investigated. Both strains were isolated from the deep oligotrophic Krubera-Voronja Cave and were found to be highly active against both Gram-positive and Gram-negative bacteria. Genome mining revealed a high number of biosynthetic gene clusters in the cave strains: 21 for strain 23TSA30-6 and 19 for strain 28ISP30-2. Single clusters encoding the biosynthesis of phosphonate, terpene, and siderophore, as well as a single trans-AT polyketide synthase/non-ribosomal peptide synthetase, were identified in both genomes. The most numerous clusters were assigned to the biosynthetic pathways of non-ribosomal peptides and ribosomally synthesized and post-translationally modified peptides. Although four non-ribosomal peptide synthetase gene clusters were predicted to be involved in the biosynthesis of known compounds (fusaricidin, polymyxin B, colistin A, and tridecaptin) of the genus Paenibacillus, discrepancies in the structural organization of the clusters, as well as in the substrate specificity of some adenylation domains, were detected between the reference pathways and the clusters in our study. Among the clusters involved in the biosynthesis of ribosomally synthesized peptides, only one was predicted to be involved in the biosynthesis of a known compound: paenicidin B. Most biosynthetic gene clusters in the genomes of the cave strains showed a low similarity with the reference pathways and were predicted to represent novel biosynthetic pathways. In addition, the cave strains differed in their potential to encode the biosynthesis of a few unique, previously unknown compounds (class II lanthipeptides and three non-ribosomal peptides). The phenotypic characterization of proteinaceous and volatile compounds produced by strains 23TSA30-6 and 28ISP30-2 was also performed, and the results were compared with those of genome mining.
Non-synonymous Substitutions in HIV-1 GAG Are Frequent in Epitopes Outside the Functionally Conserved Regions and Associated With Subtype Differences
Authors: Olusola Babatunde A. , Olaleye David O. , Odaibo Georgina N.
Published: January 2021

In 2019, 38 million people lived with HIV-1 infection resulting in 690,000 deaths. Over 50% of this infection and its associated deaths occurred in Sub-Saharan Africa. The West African region is a known hotspot of the HIV-1 epidemic. There is a need to develop an HIV-1 vaccine if the HIV epidemic would be effectively controlled. Few protective cytotoxic T Lymphocytes (CTL) epitopes within the HIV-1 GAG (HIV_gagconsv) have been previously identified to be functionally conserved among the HIV-1 M group. These epitopes are currently the focus of universal HIV-1 T cell-based vaccine studies. However, these epitopes’ phenotypic and genetic properties have not been observed in natural settings for HIV-1 strains circulating in the West African region. This information is critical as the usefulness of universal HIV-1 vaccines in the West African region depends on these epitopes’ occurrence in strains circulating in the area. This study describes non-synonymous substitutions within and without HIV_gagconsv genes isolated from 10 infected Nigerians at the early stages of HIV-1 infection. Furthermore, we analyzed these substitutions longitudinally in five infected individuals from the early stages of infection till after seroconversion. We identified three non-synonymous substitutions within HIV_gagconsv genes isolated from early HIV infected individuals. Fourteen and nineteen mutations outside the HIV_gagconsv were observed before and after seroconversion, respectively, while we found four mutations within the HIV_gagconsv. These substitutions include previously mapped CTL epitope immune escape mutants. CTL immune pressure likely leaves different footprints on HIV-1 GAG epitopes within and outside the HIV_gagconsv. This information is crucial for universal HIV-1 vaccine designs for use in the West African region.
From Metabolite to Metabolome: Metabolomics Applications in Plasmodium Research
Authors: Yu Xinyu , Feng Gaoqian , Zhang Qingfeng , Cao Jun
Published: January 2021

Advances in research over the past few decades have greatly improved metabolomics-based approaches in studying parasite biology and disease etiology. This improves the investigation of varied metabolic requirements during life stages or when following transmission to their hosts, and fulfills the demand for improved diagnostics and precise therapeutics. Therefore, this review highlights the progress of metabolomics in malaria research, including metabolic mapping of Plasmodium vertebrate life cycle stages to investigate antimalarials mode of actions and underlying complex host-parasite interactions. Also, we discuss current limitations as well as make several practical suggestions for methodological improvements which could drive metabolomics progress for malaria from a comprehensive perspective.
Early Segmental White Matter Fascicle Microstructural Damage Predicts the Corresponding Cognitive Domain Impairment in Cerebral Small Vessel Disease Patients by Automated Fiber Quantification
Authors: Huang Lili , Chen Xin , Sun Wenshan , Chen Haifeng , Ye Qing , Yang Dan , Li Mengchun , Luo Caimei , Ma Junyi , Shao Pengfei , Xu Hengheng , Zhang Bing , Zhu Xiaolei , Xu Yun
Published: January 2021

Objective: To characterize earlier damage pattern of white matter (WM) microstructure in cerebral small vessel disease (CSVD) and its relationship with cognitive domain dysfunction.

Methods: A total of 144 CSVD patients and 100 healthy controls who underwent neuropsychological measurements and diffusion tensor imaging (DTI) examination were recruited. Cognitive function, emotion, and gait were assessed in each participant. The automated fiber quantification (AFQ) technique was used to extract different fiber properties between groups, and partial correlation and general linear regression analyses were performed to assess the relationship between position-specific WM microstructure and cognitive function.

Results: Specific segments in the association fibers, commissural WM regions of interest (ROIs), and projection fibers were damaged in the CSVD group [P < 0.05, family-wise error (FWE) correction], and these damaged segments showed interhemispheric symmetry. In addition, the damage to specific tract profiles [including the posteromedial component of the right cingulum cingulate (CC), the occipital lobe portion of the callosum forceps major, the posterior portion of the left superior longitudinal fasciculus (SLF), and the bilateral anterior thalamic radiation (ATR)] was related to the dysfunction in specific cognitive domains. Among these tracts, we found the ATR to be the key set of tracts whose profiles were most associated with cognitive dysfunction. The left ATR was a specific fiber bundle associated with episode memory and language function, whereas the fractional anisotropy (FA) values of the intermediate component of the right ATR were negatively correlated with executive function and gait evaluation. It should be noted that the abovementioned relationships could not survive the Bonferroni correction (p < 0.05/27), so we chose more liberal uncorrected statistical thresholds.

Conclusions: Damage to the WM fiber bundles showed extensive interhemispheric symmetry and was limited to particular segments in CSVD patients. Disruption of strategically located fibers was associated with different cognitive deficits, especially the bilateral ATR.
TRPV1 Supports Axogenic Enhanced Excitability in Response to Neurodegenerative Stress
Authors: Risner Michael L. , McGrady Nolan R. , Boal Andrew M. , Pasini Silvia , Calkins David J.
Published: January 2021

Early progression in neurodegenerative disease involves challenges to homeostatic processes, including those controlling axonal excitability and dendritic organization. In glaucoma, the leading cause of irreversible blindness, stress from intraocular pressure (IOP) causes degeneration of retinal ganglion cells (RGC) and their axons which comprise the optic nerve. Previously, we discovered that early progression induces axogenic, voltage-gated enhanced excitability of RGCs, even as dendritic complexity in the retina reduces. Here, we investigate a possible contribution of the transient receptor potential vanilloid type 1 (TRPV1) channel to enhanced excitability, given its role in modulating excitation in other neural systems. We find that genetic deletion of Trpv1 (Trpv1−/−) influences excitability differently for RGCs firing continuously to light onset (αON-Sustained) vs. light offset (αOFF-Sustained). Deletion drives excitability in opposing directions so that Trpv1−/− RGC responses with elevated IOP equalize to that of wild-type (WT) RGCs without elevated IOP. Depolarizing current injections in the absence of light-driven presynaptic excitation to directly modulate voltage-gated channels mirrored these changes, while inhibiting voltage-gated sodium channels and isolating retinal excitatory postsynaptic currents abolished both the differences in light-driven activity between WT and Trpv1−/− RGCs and changes in response due to IOP elevation. Together, these results support a voltage-dependent, axogenic influence of Trpv1−/− with elevated IOP. Finally, Trpv1−/− slowed the loss of dendritic complexity with elevated IOP, opposite its effect on axon degeneration, supporting the idea that axonal and dendritic degeneration follows distinctive programs even at the level of membrane excitability.
From Topological Analyses to Functional Modeling: The Case of Hippocampus
Authors: Dabaghian Yuri
Published: January 2021

Topological data analyses are widely used for describing and conceptualizing large volumes of neurobiological data, e.g., for quantifying spiking outputs of large neuronal ensembles and thus understanding the functions of the corresponding networks. Below we discuss an approach in which convergent topological analyses produce insights into how information may be processed in mammalian hippocampus—a brain part that plays a key role in learning and memory. The resulting functional model provides a unifying framework for integrating spiking data at different timescales and following the course of spatial learning at different levels of spatiotemporal granularity. This approach allows accounting for contributions from various physiological phenomena into spatial cognition—the neuronal spiking statistics, the effects of spiking synchronization by different brain waves, the roles played by synaptic efficacies and so forth. In particular, it is possible to demonstrate that networks with plastic and transient synaptic architectures can encode stable cognitive maps, revealing the characteristic timescales of memory processing.
The Use of Vitamin K2 in Patients With Parkinson's Disease and Mitochondrial Dysfunction (PD-K2): A Theranostic Pilot Study in a Placebo-Controlled Parallel Group Design
Authors: Prasuhn Jannik , Kasten Meike , Vos Melissa , König Inke R. , Schmid Sebastian M. , Wilms Britta , Klein Christine , Brüggemann Norbert
Published: January 2021

Background: Despite rapid advances in research on Parkinson's disease (PD), in particular in the elucidation of genetic contributions, no disease-modifying therapy has become available to date.

Objectives: In the proposed project, we aim to investigate the potential effects of vitamin K2 (long-chain menaquinone 7, MK-7) in genetically determined PD with mitochondrial dysfunction.

Methods: A total of 130 study participants (26 biallelic Parkin/PINK1 mutation carriers, 52 sporadic PD patients, and 52 healthy controls) will receive the trial medication (MK-7 or placebo for 1 week). 31P-Magnetic resonance spectroscopy imaging of the forebrain and basal ganglia (31P-MRSI, primary endpoint) as well as other advanced neuroimaging methods, clinical assessment, including quantitative movement analysis, and biomarker sampling will be applied pre- and post-intervention.

Innovation: The proposed project is highly translational as it builds on compelling mechanistic data from animal studies as well as on a small preliminary data set in humans. Patients are selected based on their mutation-related mitochondrial dysfunction and compared to disease and a healthy control group in a personalized medicine approach. We will further investigate how neuroimaging and blood-derived biomarkers can predict individual treatment response in sporadic PD.

Clinical trial registration: This study was registered at the German Clinical Trial Registry (DRKS, DRKS00019932) on the 19th of December 2019.
An Electromyographic Analysis of the Effects of Cognitive Fatigue on Online and Anticipatory Action Control
Authors: Salomone Mick , Burle Boris , Fabre Ludovic , Berberian Bruno
Published: January 2021

Cognitive fatigue is a problem for the safety of critical systems (e.g., aircraft) as it can lead to accidents, especially during unexpected events. In order to determine the extent to which it disrupts adaptive capabilities, we evaluated its effect on online and anticipatory control. Despite numerous studies conducted to determine its effects, the exact mechanism(s) affected by fatigue remains to be clarified. In this study, we used distribution and electromyographic analysis to assess whether cognitive fatigue increases the capture of the incorrect automatic response or if it impairs its suppression (online control), and whether the conflict adaptation effect is reduced (anticipatory control). To this end, we evaluated the evolution of the performance over time during the Simon task, a classic conflict task that elicits incorrect automatic responses. To accentuate the presence of fatigue during the Simon task, two groups previously performed a dual-task with two different cognitive load levels to create two different levels of fatigue. The results revealed that time on task impaired online control by disrupting the capacity to suppress the incorrect response but leaving unaffected the expression of the automatic response. Furthermore, participants emphasized speed rather than accuracy with time on task, with in addition more fast guesses, suggesting that they opted for a less effortful response strategy. As the implementation of the suppression mechanism requires cognitive effort, the conjunction of these results suggests that the deficits observed may be due to disengagement of effort over time rather than reflecting an incapacity to make an effort.
Astragalin Alleviates Neuropathic Pain by Suppressing P2X4-Mediated Signaling in the Dorsal Root Ganglia of Rats
Authors: Wang Mengke , Cai Xia , Wang Yueying , Li Shizhen , Wang Na , Sun Rui , Xing Jingming , Liang Shangdong , Liu Shuangmei
Published: January 2021

Neurologic damage often leads to neuropathic pain, for which there are no effective treatments owing to its complex pathogenesis. The purinergic receptor P2X4 is closely associated with neuropathic pain. Astragalin (AST), a compound that is used in traditional Chinese medicine, has protective effects against allergic dermatitis and neuronal injury, but its mechanism of action is not well understood. The present study investigated whether AST can alleviate neuropathic pain in a rat model established by chronic constriction injury (CCI) to the sciatic nerve. The model rats exhibited pain behavior and showed increased expression of P2X4 and the activated satellite glial cell (SGC) marker glial fibrillary acidic protein in dorsal root ganglia (DRG). AST treatment partly abrogated the upregulation of P2X4, inhibited SGC activation, and alleviated pain behavior in CCI rats; it also suppressed ATP-activated currents in HEK293 cells overexpressing P2X4. These data demonstrate that AST relieves neuropathic pain by inhibiting P2X4 and SGC activation in DRG, highlighting its therapeutic potential for clinical pain management.
Effects of Constitutive and Acute Connexin 36 Deficiency on Brain-Wide Susceptibility to PTZ-Induced Neuronal Hyperactivity
Authors: Brunal Alyssa A. , Clark Kareem C. , Ma Manxiu , Woods Ian G. , Pan Y. Albert
Published: January 2021

Connexins are transmembrane proteins that form hemichannels allowing the exchange of molecules between the extracellular space and the cell interior. Two hemichannels from adjacent cells dock and form a continuous gap junction pore, thereby permitting direct intercellular communication. Connexin 36 (Cx36), expressed primarily in neurons, is involved in the synchronous activity of neurons and may play a role in aberrant synchronous firing, as seen in seizures. To understand the reciprocal interactions between Cx36 and seizure-like neural activity, we examined three questions: (a) does Cx36 deficiency affect seizure susceptibility, (b) does seizure-like activity affect Cx36 expression patterns, and (c) does acute blockade of Cx36 conductance increase seizure susceptibility. We utilize the zebrafish pentylenetetrazol [PTZ; a GABA(A) receptor antagonist] induced seizure model, taking advantage of the compact size and optical translucency of the larval zebrafish brain to assess how PTZ affects brain-wide neuronal activity and Cx36 protein expression. We exposed wild-type and genetic Cx36-deficient (cx35.5-/-) zebrafish larvae to PTZ and subsequently mapped neuronal activity across the whole brain, using phosphorylated extracellular-signal-regulated kinase (pERK) as a proxy for neuronal activity. We found that cx35.5-/- fish exhibited region-specific susceptibility and resistance to PTZ-induced hyperactivity compared to wild-type controls, suggesting that genetic Cx36 deficiency may affect seizure susceptibility in a region-specific manner. Regions that showed increased PTZ sensitivity include the dorsal telencephalon, which is implicated in human epilepsy, and the lateral hypothalamus, which has been underexplored. We also found that PTZ-induced neuronal hyperactivity resulted in a rapid reduction of Cx36 protein levels within 30 min. This Cx36 reduction persists after 1-h of recovery but recovered after 3–6 h. This acute downregulation of Cx36 by PTZ is likely maladaptive, as acute pharmacological blockade of Cx36 by mefloquine results in increased susceptibility to PTZ-induced neuronal hyperactivity. Together, these results demonstrate a reciprocal relationship between Cx36 and seizure-associated neuronal hyperactivity: Cx36 deficiency contributes region-specific susceptibility to neuronal hyperactivity, while neuronal hyperactivity-induced downregulation of Cx36 may increase the risk of future epileptic events.
AAV Targeting of Glial Cell Types in the Central and Peripheral Nervous System and Relevance to Human Gene Therapy
Authors: O’Carroll Simon J. , Cook William H. , Young Deborah
Published: January 2021

Different glial cell types are found throughout the central (CNS) and peripheral nervous system (PNS), where they have important functions. These cell types are also involved in nervous system pathology, playing roles in neurodegenerative disease and following trauma in the brain and spinal cord (astrocytes, microglia, oligodendrocytes), nerve degeneration and development of pain in peripheral nerves (Schwann cells, satellite cells), retinal diseases (Müller glia) and gut dysbiosis (enteric glia). These cell type have all been proposed as potential targets for treating these conditions. One approach to target these cell types is the use of gene therapy to modify gene expression. Adeno-associated virus (AAV) vectors have been shown to be safe and effective in targeting cells in the nervous system and have been used in a number of clinical trials. To date, a number of studies have tested the use of different AAV serotypes and cell-specific promoters to increase glial cell tropism and expression. However, true glial-cell specific targeting for a particular glial cell type remains elusive. This review provides an overview of research into developing glial specific gene therapy and discusses some of the issues that still need to be addressed to make glial cell gene therapy a clinical reality.
Perceptual Behavior Depends Differently on Pupil-Linked Arousal and Heartbeat Dynamics-Linked Arousal in Rats Performing Tactile Discrimination Tasks
Authors: Liu Yuxiang , Narasimhan Shreya , Schriver Brian J. , Wang Qi
Published: January 2021

Several physiology signals, including heart rate and pupil size, have been widely used as peripheral indices of arousal to evaluate the effects of arousal on brain functions. However, whether behavior depends differently on arousal indexed by these physiological signals remains unclear. We simultaneously recorded electrocardiogram (ECG) and pupil size in head-fixed rats performing tactile discrimination tasks. We found both heartbeat dynamics and pupil size co-varied with behavioral outcomes, indicating behavior was dependent upon arousal indexed by the two physiological signals. To estimate the potential difference between the effects of pupil-linked arousal and heart rate-linked arousal on behavior, we constructed a Bayesian decoder to predict animals' behavior from pupil size and heart rate prior to stimulus presentation. The performance of the decoder was significantly better when using both heart rate and pupil size as inputs than when using either of them alone, suggesting the effects of the two arousal systems on behavior are not completely redundant. Supporting this notion, we found that, on a substantial portion of trials correctly predicted by the heart rate-based decoder, the pupil size-based decoder failed to correctly predict animals' behavior. Taken together, these results suggest that pupil-linked and heart rate-linked arousal systems exert different influences on animals' behavior.
Using Genome-Wide Predictions to Assess the Phenotypic Variation of a Barley (Hordeum sp.) Gene Bank Collection for Important Agronomic Traits and Passport Information
Authors: Jiang Yong , Weise Stephan , Graner Andreas , Reif Jochen C.
Published: January 2021

Genome-wide predictions are a powerful tool for predicting trait performance. Against this backdrop we aimed to evaluate the potential and limitations of genome-wide predictions to inform the barley collection of theFederal ex situ Genebank for Agricultural and Horticultural Cropswith phenotypic data on complex traits including flowering time, plant height, thousand grain weight, as well as on growth habit and row type. We used previously published sequence data, providing information on 306,049 high-quality SNPs for 20,454 barley accessions. The prediction abilities of the two unordered categorical traits row type and growth type as well as the quantitative traits flowering time, plant height and thousand grain weight were investigated using different cross validation scenarios. Our results demonstrate that the unordered categorical traits can be predicted with high precision. In this way genome-wide prediction can be routinely deployed to extract information pertinent to the taxonomic status of gene bank accessions. In addition, the three quantitative traits were also predicted with high precision, thereby increasing the amount of information available for genotyped but not phenotyped accessions. Deeply phenotyped core collections, such as the barley 1,000 core set of the IPK Gatersleben, are a promising training population to calibrate genome-wide prediction models. Consequently, genome-wide predictions can substantially contribute to increase the attractiveness of gene bank collections and help evolve gene banks into bio-digital resource centers.
Advances in Breeding for Mixed Cropping – Incomplete Factorials and the Producer/Associate Concept
Authors: Haug Benedikt , Messmer Monika M. , Enjalbert Jérôme , Goldringer Isabelle , Forst Emma , Flutre Timothée , Mary-Huard Tristan , Hohmann Pierre
Published: January 2021

Mixed cropping has been suggested as a resource-efficient approach to meet high produce demands while maintaining biodiversity and minimizing environmental impact. Current breeding programs do not select for enhanced general mixing ability (GMA) and neglect biological interactions within species mixtures. Clear concepts and efficient experimental designs, adapted to breeding for mixed cropping and encoded into appropriate statistical models, are lacking. Thus, a model framework for GMA and SMA (specific mixing ability) was established. Results of a simulation study showed that an incomplete factorial design combines advantages of two commonly used full factorials, and enables to estimate GMA, SMA, and their variances in a resource-efficient way. This model was extended to the Producer (Pr) and Associate (As) concept to exploit additional information based on fraction yields. It was shown that the Pr/As concept allows to characterize genotypes for their contribution to total mixture yield, and, when relating to plant traits, allows to describe biological interaction functions (BIF) in a mixed crop. Incomplete factorial designs show the potential to drastically improve genetic gain by testing an increased number of genotypes using the same amount of resources. The Pr/As concept can further be employed to maximize GMA in an informed and efficient way. The BIF of a trait can be used to optimize species ratios at harvest as well as to extend our understanding of competitive and facilitative interactions in a mixed plant community. This study provides an integrative methodological framework to promote breeding for mixed cropping.
Participating in Online Museum Communities: An Empirical Study of Taiwan’s Undergraduate Students
Authors: Chen Tien-Li , Lai Wei-Chun , Yu Tai-Kuei
Published: January 2021

With the worldwide spread of the Internet, human activity has become permeated by digital media, which shapes communication and interaction and speeds up the improvement of the experience and diffusion of museum exhibitions. Contemporary museums must understand their audiences, especially with respect to online preferences and surfing involvement experiences. Museums are changing in an effort to attract young netizens to access and use museum resources. Virtual museums are increasingly using digital exhibitions to preserve and apply their collections and establishing online community platforms to interact with young people. This study investigates the underlying mechanism of online community characteristics that enhance audiences’ emotional resonance and involvement. Results from a questionnaire survey (N= 1168) of Taiwan undergraduate students show that perceived relevance and esteem improve their emotional resonance, which can attract new people and maintain existing relationships within their communities. Following flow theory, maintaining community relationship characteristics increases emotional resonance, which, in turn, enhances user involvement, but we found only small significant effects of emotional resonance on involvement. These findings illuminate the mechanism of the attitudinal relationship building and maintenance for online museum communities and advance the practical contributions of online museum community use and effects.
School and Teacher Factors That Promote Adolescents’ Bystander Responses to Social Exclusion
Authors: Mulvey Kelly Lynn , Gönültaş Seçil , Irdam Greysi , Carlson Ryan G. , DiStefano Christine , Irvin Matthew J.
Published: January 2021

Schools may be one important context where adolescents learn and shape the behaviors necessary for promoting global inclusivity in adulthood. Given the importance of bystanders in halting bullying and peer aggression, the focus of this study is on both moral judgments regarding one type of bullying, social exclusion, and factors that are associated with bystander intervention. The study includes 896 adolescents, who were 6th (N= 450,Mage= 11.73), and 9th (N= 446,Mage= 14.82) graders, approximately evenly divided by gender. Participants were primarily European–American (63.3%). Results revealed that girls and participants who perceived better relationships between students and teachers were more likely to judge exclusion to be wrong. Further, ethnic minority participants, those who were more anxious about being rejected by their teachers and reported more teacher discrimination were less likely to judge exclusion as wrong. Participants who reported more positive student–teacher relationships, perceptions of a more positive school social environment and more prior experiences of teacher discrimination were more likely to report that they would seek help for the victim. On the other hand, participants who reported being more angry about teacher rejection, experiencing either peer or teacher discrimination, and perceiving they are excluded from opportunities at school were less likely to intervene to come to the aid of a peer who is being excluded. The results document the complex interplay of school and teacher factors in shaping adolescents’ bystander responses to social exclusion. Our findings suggest that positive school climate can promote intentions to intervene. However, findings indicate that adolescents who are marginalized in their school environments, and who report experiences of rejection, exclusion or discrimination are not willing or likely to intervene to prevent others from experiencing exclusion.
The Role of Body Image on Psychosocial Outcomes in People With Diabetes and People With an Amputation
Authors: McDonald Sarah , Sharpe Louise , MacCann Carolyn , Blaszczynski Alex
Published: January 2021

Introduction

Research indicates that body image disturbance is associated with poorer psychosocial outcomes for individuals with physical health conditions, with poorest body image reported for individuals with visible bodily changes. Using White’s (2000) theoretical model of body image the present paper aimed to examine the nature of these relationships in two distinct groups: individuals with an amputation and individuals with diabetes. It was hypothesized that body image disturbance would be associated with psychosocial outcomes and would mediate the relationships between self-ideal discrepancy and personal investment in psychosocial outcomes.

Methods

Individuals with diabetes (N = 212) and individuals with an amputation (N = 227) provided details regarding their medical condition, and completed measures assessing body image, investment, self-ideal discrepancy, depression, anxiety, and quality of life. Structural equation and invariance modeling were used to test the model paths and the invariance of the model.

Results

As hypothesized, body image disturbance was found to mediate the relationships between personal investment and psychosocial outcome, and between self-ideal discrepancy and psychosocial outcome. The predicted paths were invariant across groups, although the model accounted for more variance in people with an amputation than people with diabetes.

Conclusion

Body image disturbance, personal investment, and self-ideal discrepancy are important factors contributing to psychosocial outcome for individuals with diabetes and individuals with an amputation. These findings not only confirm the validity of the model in these two groups, but they emphasize the importance of targeting body image in future psychological interventions for individuals with a health condition.
Supported Decision-Making and Paradigm Shifts: Word Play or Real Change?
Authors: Stavert Jill
Published: January 2021

Article 12(3) CRPD requires states parties to provide access by persons with disabilities to the support they may require in exercising their legal capacity. This is to ensure that the rights, will and preferences of persons with disabilities are enjoyed on an equal basis with others [Articles 12(1)(2) and (4) CRPD]. Moreover, the Committee on the Rights of Persons with Disabilities has made it clear that supported decision-making must replace substitute decision-making arrangements as these are discriminatory and deny equal enjoyment of the right to exercise of legal capacity for persons. At the same time, there is ongoing debate as to whether or not the absence of substitute decision-making regimes is essential for the non-discriminatory realization of an individual's rights, will and preferences to be achieved. To resolve this debate, however, specific attention needs to be paid to the CRPD message on what it actually means to give effect to the equal and non-discriminatory enjoyment of rights for all. In the context of persons with mental disabilities this requires looking beyond human rights simply in terms of limiting unwarranted interventions to the proactive removal of obstacles to full rights enjoyment and the creation of environments that respect and support such enjoyment. With this in mind this paper will therefore critically consider the use of supported decision-making within existing substitute decision-making regimes with particular reference to Scotland's mental health and capacity laws. It will consider the challenges this poses and whether it is indeed possible to adapt existing regimes to achieve CRPD compliance. In doing so, it is suggested that a full appreciation of the overarching CRPD message about equality and non-discrimination in the enjoyment of rights is required to bring about such compliance.
Tackling Energy Poverty Through Behavioral Change: A Pilot Study on Social Comparison Interventions in Social Housing Districts
Authors: Caballero Nicolas , Della Valle Nives
Published: January 2021

Behavioral Economics has in recent years played a key role in informing the design of non-price interventions aimed at promoting energy conservation behaviors in residential housing. Some of the most influential contributions of the discipline in an applied setting have centered around the development of norm-based interventions. The success that these interventions have had in specific contexts presents an opportunity to exploit them as tools for tackling a prevalent type of poverty at the EU level: energy poverty. Recent contributions to the literature highlight the role of inefficient energy behavior as a significant driver of this particular type of poverty, which is characterized by an inability to afford the basic energy services necessary to guarantee a decent standard of living. Therefore, the effectiveness of norm-based interventions in vulnerable populations merits further investigation to determine whether this approach can suitably address the behavioral components of energy poverty by promoting efficient energy consumption and conservation efforts. This study reports on a pilot conducted in an exemplary social housing context (located in Bolzano, Italy) with the aim to assess the effectiveness of social comparison interventions in energy vulnerable groups. Our investigated cohort covers an initial small sample of apartments with a large representatives of elderly individuals and other energy-vulnerable groups. Using a design that combines appeals to injunctive and descriptive norms embedded within In-Home Devices (IHD) in recently retrofitted homes, our objective is to set a basis for the assessment of effectiveness of these types of interventions in social housing populations. Our study seeks to provide useful methodological insights to policy makers on how to effectively design behaviorally informed interventions aimed at tackling energy poverty. Despite the current data limitations, our results do seem to suggest that uniformly applied norm-based interventions may have potentially backfiring effects in small-scale implementations. Therefore, they suggest that attention needs to be paid to household composition and pre-existing levels of consumption, when designing behavior-change interventions in these groups.

JEL Classification: C93; D03; D04; D12; D19; D91; Q40.
Proyecto de emprendimiento y economía familiar, Guasmo, 2019. Una experiencia de vinculación.
Authors: Flores Morán John Freddy , Salazar Ojeda Edgar Xavier
Published: January 2021

El artículo refiere la experiencia de ejecución del proyecto de vinculación con la colectividad, en el campo de emprendimiento y economía familiar, en la Parroquia Nuestra Señora de la Encarnación, ubicada en la Unión de Bananeros, del Guasmo Sur, de la ciudad de Guayaquil; por estudiantes de la carrera de Administración de Empresas de la Universidad Politécnica Salesiana (UPS), con monitoreo docente; teniendo como objetivo evaluar su implementación, con una metodología de tipo descriptiva, a partir de la valoración que realizaron los participantes en una encuesta tipo likert, tomada al final del periodo formativo. Sus resultados principales reflejan las opiniones de los beneficiarios en las categorías de organización, desarrollo de contenidos prácticos, a través de los cuales se alcanzó un alto nivel de participación, y donde el proceso de evaluación fue clave para mejorar la asimilación de los contenidos; la calidad de los instructores y el nivel de aprendizaje adquirido; que permitió estimular el espíritu emprendedor. Se concluye que el desarrollo de estas temáticas, dirigidas a sectores vulnerables de la sociedad, constituye una estrategia para enfrentar los altos niveles de desempleo que se viven en el país.
Liderazgo transformacional y transaccional en la autoeficacia emprendedora del turismo comunitario amazónico, caso provincia de Pastaza
Authors: Manosalvas Vaca Luis Oswaldo , Tobanda Barragán Angélica Aracely , Manosalvas Vaca Carlos Aníbal , Quevedo Amay Deisy Valeria
Published: January 2021

La presente investigación tiene como objetivo analizar la relación del  Liderazgo transformacional y transaccional con la Autoeficacia emprendedora en una comunidad indígena  ubicada en Amazonía ecuatoriana, Provincia de Pastaza, donde se desarrolló una evaluación de las características demográficas de la muestra y un análisis descriptivo de las variables de estudio, para analizar la variable liderazgo se utilizó el cuestionario multifactorial de liderazgo (Multifactor Leadership Questionnaire (MLQ), y el instrumento de autoeficacia emprendedora desarrollado por De Noble et al. (1999). Además, se midió la confiabilidad de los instrumentos con un análisis de Cronbach y mediante la correlación de Pearson se determinó que el liderazgo transformacional se relaciona de manera significativa con la autoeficacia emprendedora, sin embargo, el liderazgo transaccional no se relaciona con la autoeficacia emprendedora; los resultados indicaron que la actividad emprendedora de la comunidad tiene una influencia de carácter transformacional a través de sus emprendedores líderes, quienes apoyan el desarrollo emprendedor considerando el avance personal de los miembros, sin embargo, no se ha identificado una relación de un proceso de transacción o recompensa en las actividades de emprendimiento, esto permite generar posibles investigaciones que analicen futuros comportamientos de emprendimiento con respecto a procedimientos transaccionales y transformacionales de liderazgo en el emprendimientos en diversos contextos similares.
Urban e-Grocery Distribution Design in Pamplona (Spain) Applying an Agent-Based Simulation Model with Horizontal Cooperation Scenarios
Authors: Serrano-Hernandez Adrian , de la Torre Rocio , Cadarso Luis , Faulin Javier
Published: January 2021

E-commerce has boosted in the last decades because of the achievements of the information and telecommunications technology along with the changes in the society life-style. More recently, the groceries online purchase (or e-grocery), has also prevailed as a way of making the weekly shopping, particularly, the one including fresh vegetables and fruit. Furthermore, this type of virtual shopping in supermarkets is gaining importance as the most efficient delivery system in cost and time. Thus, we have evaluated in this study the influence of the cooperation-based policies on costs and service quality among different supermarkets in Pamplona, Spain. Concerning methodology, first of all, we carried out a survey in Pamplona having the purpose of modelling the demand patterns about e-grocery. Second, we have developed an agent-based simulation model for generating scenarios in non-cooperative, limited cooperation, and full cooperation settings, considering the real data obtained from the survey analysis. At this manner, Vehicle Routing Problems (VRP) and Multi Depot VRPs (MDVRP) are dynamically generated and solved within the simulation framework using a biased-randomization algorithm. Finally, the results show significant reductions in distance driven and lead times when employing horizontal cooperation in e-grocery distribution.
Dynamic Shortest Paths Methods for the Time-Dependent TSP
Authors: Hansknecht Christoph , Joormann Imke , Stiller Sebastian
Published: January 2021

The time-dependent traveling salesman problem (TDTSP) asks for a shortest Hamiltonian tour in a directed graph where (asymmetric) arc-costs depend on the time the arc is entered. With traffic data abundantly available, methods to optimize routes with respect to time-dependent travel times are widely desired. This holds in particular for the traveling salesman problem, which is a corner stone of logistic planning. In this paper, we devise column-generation-based IP methods to solve the TDTSP in full generality, both for arc- and path-based formulations. The algorithmic key is a time-dependent shortest path problem, which arises from the pricing problem of the column generation and is of independent interest—namely, to find paths in a time-expanded graph that are acyclic in the underlying (non-expanded) graph. As this problem is computationally too costly, we price over the set of paths that contain no cycles of length k. In addition, we devise—tailored for the TDTSP—several families of valid inequalities, primal heuristics, a propagation method, and a branching rule. Combining these with the time-dependent shortest path pricing we provide—to our knowledge—the first elaborate method to solve the TDTSP in general and with fully general time-dependence. We also provide for results on complexity and approximability of the TDTSP. In computational experiments on randomly generated instances, we are able to solve the large majority of small instances (20 nodes) to optimality, while closing about two thirds of the remaining gap of the large instances (40 nodes) after one hour of computation.