bluuebunny/crossref_metadata_2025 huggingface dataset:
Voluntary exercise does not increase gastrointestinal motility but increases spatial memory, intestinal eNOS, Akt levels, and Bifidobacteria abundance in the microbiome
Authors: Bakonyi Peter , Kolonics Attila , Aczel Dora , Zhou Lei , Mozaffaritabar Soroosh , Molnár Kinga , László Lajos , Kutasi Balazs , Tanisawa Kumpei , Park Jonguk , Gu Yaodong , Pinho Ricardo A. , Radak Zsolt
Published: August 2023

The interaction between the gut and brain is a great puzzle since it is mediated by very complex mechanisms. Therefore, the possible interactions of the brain–exercise–intestine–microbiome axis were investigated in a control (C, N = 6) and voluntarily exercised (VE, N = 8) middle-aged rats. The endurance capacity was assessed by VO2max on the treadmill, spatial memory by the Morris maze test, gastrointestinal motility by EMG, the microbiome by 16S RNA gene amplicon sequencing, caveolae by electron microscopy, and biochemical assays were used to measure protein levels and production of reactive oxygen species (ROS). Eight weeks of voluntary running increased VO2max, and spatial memory was assessed by the Morris maze test but did not significantly change the motility of the gastrointestinal tract or production of ROS in the intestine. The protein kinase B (Akt) and endothelial nitric oxide synthase (eNOS) protein levels significantly increased in the intestine, while peroxisome proliferator–activated receptor gamma coactivator 1 alpha (PGC-1α), mitochondrial transcription factor A (TFAM), nuclear respiratory factor 1 (NFR1), SIRT1, SIRT3, nicotinamide phosphoribosyl transferase (NAMPT), and nuclear factor κB (NF-κB) did not change. On the other hand, voluntary exercise increased the number of caveolae in the smooth muscles of the intestine and relative abundance of Bifidobacteria in the microbiome, which correlated with the Akt levels in the intestine. Voluntary exercise has systemic effects and the relationship between intestinal Akt and the microbiome of the gastrointestinal tract could be an important adaptive response.
Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses
Authors: Vidal Moreno de Vega Carmen , Lemmens Diete , de Meeûs d’Argenteuil Constance , Boshuizen Berit , de Maré Lorie , Leybaert Luc , Goethals Klara , de Oliveira Jean Eduardo , Hosotani Guilherme , Deforce Dieter , Van Nieuwerburgh Filip , Devisscher Lindsey , Delesalle Cathérine
Published: August 2023

Important changes in glucose transporter (GLUT) expression should be expected if the glucose influx plays a pivotal role in fuelling or connecting metabolic pathways that are upregulated in response to exercise. The aim was to assess GLUT4, 8, and 12 dynamics in response to training and acute exercise.

Methods: Sixteen untrained Standardbred mares (3-4 year) performed an incremental SET at the start and end of 8 weeks harness training. M. pectoralis (PM) and M. vastus lateralis (VL) muscle biopsies were taken before and after each SET, allowing for comparing rest and acute samples in untrained (UT) and trained (T) condition using Western Blot for GLUT quantification and Image Pro v.10 for Blot analysis. Data were normalized against GAPDH. Basal GLUT-levels of PM versus VL were analysed with the Wilcoxon matched-pairs signed rank test. The effect of acute exercise or training was assessed using the Friedman test with a post hoc Dunn’s.

Results: Basal GLUT4 and GLUT12 protein expression were significantly higher in the VL compared to the PM (PGLUT4 = 0.031 and PGLUT12 = 0.002). Training had no effect on basal GLUT4 expression, neither in the VL (p > 0.9999), nor the PM (p > 0.9999). However, acute exercise in trained condition significantly decreased GLUT4 expression in the VL (p = 0.0148). Neither training nor acute exercise significantly changed total GLUT8 protein expression. Training significantly decreased total GLUT12 protein expression in rest biopsies, only visible in the VL (p = 0.0359). This decrease was even more prominent in the VL after acute exercise in trained condition (PVL = 0.0025).

Conclusion: The important changes seen in GLUT12 expression downregulation, both in response to training and acute exercise in the horse, the downregulation of GLUT4 expression after acute exercise in trained condition and the lack of differential shifts in GLUT8 expression in any of the studied conditions, questions the importance of glucose as substrate to fuel training and exercise in healthy horses. These findings encourage to further explore alternative fuels for their involvement in equine muscular energetics.
Participation in the classroom as a basis for democracy education? A conceptual analysis of the concept of student participation
Authors: Heid Helmut , Jüttler Michael , Kärner Tobias
Published: August 2023

In our conceptual analysis, we focus on the concept of student participation and argue for a differentiated consideration of the same. To this end, we elaborate on constituent elements and first address the definitional understanding of the concept considered as well as the participation postulate. Furthermore, we name and discuss possible purposes and qualities of participatory practice and we give an overview of the established conceptualization of participation by means of a stage model and the interdependence of autonomy and heteronomy expressed in this model. We then address participatory fields in the classroom, concrete practices of participatory decision-making, prerequisites and implications of participatory classroom practices, and possible scopes of participatory influence.
Social ecological resources for youths living with HIV in western Uganda
Authors: Vindevogel Sofie , Kimera Emmanuel
Published: August 2023

Introduction

The adversities faced by youths living with HIV (YLWH) are manifold, resulting not only from the health impact but also from society’s response to HIV and the people living with it. This study sought to explore these youths’ perceptions and representations of what promotes resilience.

Methods

Photovoice methodology was chosen to elicit first-person accounts that are grounded in lived experience and experiential knowledge. Eleven young people, boys and girls aged 14–21 living in western Uganda, participated in seven group sessions aimed at imagining, producing and discussing visual stories about what fosters resilience in the face of HIV-related adversity. The visual stories were subjected to inductive content analysis by the participants, and then thematically analyzed and interpreted by the researchers using the theoretical framework of social-ecological resilience.

Results

We found that participants experience well-being amidst HIV-related adversity through managing tensions in material resources, sense of identity, power and control in their lives, cultural adherence, relationships, sense of cohesion and social justice.

Discussion

The findings add to the body of knowledge on youth resilience in Sub-Saharan Africa by documenting multisystemic resources for YLWH in Ugandan communities. The findings further show that resources are highly incidental and situational, neither widely available nor structurally embedded in society. The study therefore informs the global HIV/AIDS agenda to spur ecologies of resilience around YLWH.
The effects of viewing by scrolling on a small screen on the encoding of objects into visual long-term memory
Authors: Sho Hayato , Morita Hiromi
Published: August 2023

The perception of an image obtained by scrolling through a small screen can differ from the typical perception of a wide visual field in a stable environment. However, we do not fully understand image perception by scrolling on a small screen based on psychological knowledge of visual perception and cognition of images. This study investigated how screen size limitations and image shifts caused by scrolling affect image encoding in visual long-term memory. Participants explored the stimulus images under three conditions. Under the scrolling condition, they explored the image through a small screen. Under the moving-window condition, they explored the image by moving the screen over a masked image; this is similar to looking through a moving peephole. Under the no-window condition, participants were able to view the entire image simultaneously. Each stimulus comprised 12 objects. After 1 h, the samples were tested for object recognition. Consequently, the memory retention rate was higher in the scrolling and moving-window conditions than in the no-window condition, and no difference was observed between the scrolling and moving-window conditions. The time required by participants to explore the stimulus was shorter under the no-window condition. Thus, encoding efficiency (i.e., the rate of encoding information into memory in a unit of time) did not differ among the three conditions. An analysis of the scan trace of the scrolling and window movements in relation to the image revealed differences between the scrolling and moving-window conditions in terms of the scan’s dynamic features. Moreover, a negative correlation was observed between the memory retention rate and image-scrolling speed. We conclude that perceiving images by scrolling on a small screen enables better memory retention than that obtained through whole-image viewing if the viewing time is not limited. We suggest that viewing through a small screen is not necessarily disadvantageous for memory encoding efficiency depending on the presentation mode, and the results show that participants who scrolled fast tended to have worse memory retention. These findings can impact school education and thus suggest that the use of mobile devices in learning has some merit from the viewpoint of cognitive psychology.
The impact of automatic speech recognition technology on second language pronunciation and speaking skills of EFL learners: a mixed methods investigation
Authors: Sun Weina
Published: August 2023

Introduction

This study employed an explanatory sequential design to examine the impact of utilizing automatic speech recognition technology (ASR) with peer correction on the improvement of second language (L2) pronunciation and speaking skills among English as a Foreign Language (EFL) learners. The aim was to assess whether this approach could be an effective tool for enhancing L2 pronunciation and speaking abilities in comparison to traditional teacher-led feedback and instruction.

Methods

A total of 61 intermediate-level Chinese EFL learners were randomly assigned to either a control group (CG) or an experimental group (EG). The CG received conventional teacher-led feedback and instruction, while the EG used ASR technology with peer correction. Data collection involved read-aloud tasks, spontaneous conversations, and IELTS speaking tests to evaluate L2 pronunciation and speaking skills. Additionally, semi-structured interviews were conducted with a subset of the participants to explore their perceptions of the ASR technology and its impact on their language learning experience.

Results

The quantitative analysis of the collected data demonstrated that the EG outperformed the CG in all measures of L2 pronunciation, including accentedness and comprehensibility. Furthermore, the EG exhibited significant improvements in global speaking skill compared to the CG. The qualitative analysis of the interviews revealed that the majority of the participants in the EG found the ASR technology to be beneficial in enhancing their L2 pronunciation and speaking abilities.

Discussion

The results of this study suggest that the utilization of ASR technology with peer correction can be a potent approach in enhancing L2 pronunciation and speaking skills among EFL learners. The improved performance of the EG compared to the CG in pronunciation and speaking tasks demonstrates the potential of incorporating ASR technology into language learning environments. Additionally, the positive feedback from the participants in the EG underscores the value of using ASR technology as a supportive tool in language learning classrooms.
How is occupational identity affected by the family environment of publicly funded students in local normal colleges? A moderated mediation model
Authors: Zhang Jia , Liu Yang , Mao Zhi , Xiao Bei
Published: August 2023

The occupational identity of local publicly funded students is associated with the stability of rural teachers. To discuss the influence mechanisms of family environment, psychological capital, and professional commitment on occupational identity, this study examined 395 local publicly funded students with the occupational identity scale, psychological capital scale, professional commitment scale compiled, and family environment scale in China. We found no significant difference in terms of sex, source of birth, only children or not, and from teachers' families or not. Junior students' occupational identity was significantly higher than that of freshmen. In addition, we found that psychological capital plays a total mediation role between family environment and occupational identity. Psychological capital explains the influence of family environment on occupational identity. While the moderating role of professional commitment on the family environment and psychological capital is not supported in this study, it positively moderates the mediation role of psychological capital and occupational identity. Overall, this study will be significant in improving the training quality of local publicly funded students.
Implications of built and social environments on the academic success among African American youth: testing Strong African American Families intervention effects on parental academic racial socialization
Authors: Murry Velma McBride , Gonzalez Catherine M. , Debreaux Marlena L. , Coates Erica E. , Berkel Cady
Published: August 2023

Studies exploring widening academic disparities have highlighted the role of racialized school settings, which have given way to incidents of discrimination and unfair treatment for students of color, disproportionately affecting African American youth. Research also shows that family-based preventive interventions may avert negative outcomes for this population through the promotion of protective socialization practices. Consequently, the current study tests the efficacy of a culturally tailored preventive family-based program to foster induced changes in academic promotive parenting practices that prepare youth to advance academically by navigating negative race-related experiences in school settings. Data collected over four time periods from the Strong African American Families (SAAF) efficacy trial (Murry and Brody, 2004) with 667 African American families in rural Georgia were used for this study. Structural equation modeling analyses demonstrated that the SAAF program was associated with positive intervention induced changes in parental academic race-related socialization, which in turn, was indirectly associated with reduced school compromising behaviors through the enhancement of racial pride. While discrimination compromised academic success, our findings highlight the protective nature of racial pride in dissuading academic failure and school dropout through the promotion of academic success. This study confirms that a family-based prevention program holds promise to address academic disparities through the enhancement of parenting and youth protective processes that buffer youth from succumbing to racialized social environments such as schools. Implications for research, educational policy, and preventive interventions are discussed.
Are we willing to share what we believe is true? Factors influencing susceptibility to fake news
Authors: Piksa Michal , Noworyta Karolina , Gundersen Aleksander B. , Kunst Jonas , Morzy Mikolaj , Piasecki Jan , Rygula Rafal
Published: August 2023

Background

The contemporary media landscape is saturated with the ubiquitous presence of misinformation. One can point to several factors that amplify the spread and dissemination of false information, such as blurring the line between expert and layman's opinions, economic incentives promoting the publication of sensational information, the zero cost of sharing false information, and many more. In this study, we investigate some of the mechanisms of fake news dissemination that have eluded scientific scrutiny: the evaluation of veracity and behavioral engagement with information in light of its factual truthfulness (either true or false), cognitive utility (either enforcing or questioning participants' beliefs), and presentation style (either sober or populistic).

Results

Two main results emerge from our experiment. We find that the evaluation of veracity is mostly related to the objective truthfulness of a news item. However, the probability of engagement is more related to the congruence of the information with the participants' preconceived beliefs than to objective truthfulness or information presentation style.

Conclusion

We conclude a common notion that the spread of fake news can be limited by fact-checking and educating people might not be entirely true, as people will share fake information as long as it reduces the entropy of their mental models of the world. We also find support for the Trojan Horse hypothesis of fake news dissemination.
The effect of internalized stigmatization on care burden in adolescent psychiatric patients and their parents
Authors: Gümüş Funda , Kaçan Havva
Published: August 2023

Introduction

The purpose of this study was to determine the effect of internalized stigma perceived by adolescents with mental disorders and their families, on the burden of care on their families.

Methods

The study was conducted in a descriptive and correlational desing with adolescents and their parents who applied to the child and adolescent psychiatry outpatient clinic between March 1 and June 1, 2022. A total 101 adolescents and 101 parents who met the sampling criteria and agreed to participate in the study. Only 1 parent per adolescent was included in the study.

Results

It was found that the mean age of the adolescents was 15.05  ±  (1.80), 57.4% were female and 56.4% were secondary school graduates, and 26.7% of the adolescents, all of whom were unemployed, were diagnosed with a mood disorder, 50.5% had received outpatient treatment previously, and 22.8% had chronic diseases. It was found that the mean age of the parents who participated in the study was 45.53  ±  (6.48), 77.1% were female and 28.7% were secondary school graduates. Also, 22.8% of the parents had a chronic disease, 28.7% had a chronic disease in their family and 32.7% had another family member in need of care, and 17.0% of the parents had received training on mental disorders previously. It was found that the parents’ total means ZBI score was 42.74  ±  (11.92). When the ZBI total scores were examined in terms of sociodemographic variables, no significant differences were found between the groups according to the variables. There was only a weak, positive and significant relationship between the age of the parents and in the present study, it was found that adolescents with mental disorders and their families experienced internalized stigmatization and this stigmatization increased the care burden on parents.

Discussion

Mental health and psychiatric nurses can facilitate this population’s access to treatment by planning and implementing psychosocial interventions to reduce the internalized stigma of children and adolescents and their families.
Treating depression in clinical practice: new insights on the multidisciplinary use of trazodone
Authors: Albert Umberto , Tomasetti Carmine , Marra Camillo , Neviani Francesca , Pirani Alessandro , Taddeo Daiana , Zanetti Orazio , Maina Giuseppe
Published: August 2023

Depression is estimated to be a leading contributor to the global mental health-related burden. The determinants of this huge prevalence lie in the fact that depressive symptoms may be comorbid in a wide variety of disorders, thus complicating and exacerbating their clinical framework. This makes the treatment of depressive symptoms difficult, since many pharmacological interactions should be considered by physicians planning therapy. Hence, depression still represents a challenge for both psychiatrists and other clinicians, in terms of its high rates of relapse and resistance despite well-established protocols. It is also complicated by the well-known latency in its complete response to current antidepressant treatments. In this context, the search for new strategies regarding antidepressant treatment is mandatory. Revising the use of “old” pharmacotherapies by considering their specific features may help to perfecting the treatment of depression, both in its standalone psychiatric manifestation and in the framework of other clinical conditions. Using a nominal group technique approach, the results of a consensus of expert physicians regarding the possible use of trazodone as a valuable strategy for addressing the “real world” unmet needs of depression treatment in different fields (psychiatry, primary care, neurology and geriatrics) is herein provided. This idea is based on the unique characteristics of this drug which delivers a more rapid antidepressant action as compared to other selective serotonin reuptake inhibitors. It also has pharmacodynamic malleability (i.e., the possibility of exerting different effects on depressive symptoms at different dosages) and pharmacokinetic tolerability (i.e., the possibility of being used as an add-on to other antidepressants with scarce interaction and achieving complimentary effects) when used in the milieu of other drugs in treating comorbid depressive symptoms. Moreover, the large number of formulations available permits finite dosage adjustments, and the use of trazodone for specific pathologies, such as dysphagia. Therefore, although additional studies exploring the real-world conditions of antidepressant treatment are warranted, experts agree on the idea that depressive disorder, in both its standalone and its comorbid manifestations, may surely take advantage of the particular characteristics of trazodone, thus attempting to reach the greatest effectiveness in different contexts.
Combating stigma in autism research through centering autistic voices: a co-interview guide for qualitative research
Authors: Kaplan-Kahn Elizabeth A. , Caplan Reid
Published: August 2023

As autism has gained increased attention in the past few decades, autistic advocates have adopted the phrase “Nothing about us without us,” illustrating the idea that autistic people should be centered in all conversations regarding autism. However, in a large portion of autism research, autistic people are still not meaningfully engaged throughout the research process, leading to continued stigma in research through biased methods. Thus, stigma about autism influences not only the content of autism research, but the ways in which neurotypical people conduct research alongside (or without) autistic people, ultimately resulting in less valid conclusions or research that actively harms the autistic community. One way to address this stigma is through involving autistic individuals as equal partners in the research process, such as by including autistic co-interviewers in qualitative studies of autistic people. In this perspectives piece, we will highlight the benefits of participatory research practices within qualitative research. Furthermore, we will outline methods for conducting co-interviews with autistic research partners and share insights from our experiences implementing this practice. We hope this piece provides researchers the practical resources and inspiration to continue working toward decreasing the stigma surrounding autism in research spaces.
Nodal tumor volume as a prognostic factor for oral squamous cell carcinoma—a systematic review
Authors: Bernasconi Mischa , Bilic Ante , Kauke-Navarro Martin , Safi Ali-Farid
Published: August 2023

Oral squamous cell carcinoma (OSCC) is a complex disease with a high potential for lymph node metastasis and poor survival rates. Accurate nodal staging is crucial for prognostic assessment and treatment planning in OSCC. Recent research has suggested that nodal tumor volume (NTV) may be a more accurate indicator of nodal disease burden than traditional staging methods. However, the prognostic significance of NTV in OSCC remains unclear. This systematic review aims to evaluate the existing evidence on the relationship between NTV and prognosis in OSCC. A comprehensive search of electronic databases was conducted, and studies meeting inclusion criteria were critically appraised and synthesized. Our review identified 23 studies that investigated the prognostic significance of NTV in OSCC. The majority of studies reported that larger NTV was associated with poorer survival outcomes, although the strength of the association varied. The review also identified several areas for future research, including the standardization of NTV measurement and the integration of NTV into the broader landscape of OSCC management. In conclusion, our review suggests that NTV holds promise as a novel prognostic factor in OSCC, but more research is needed to fully elucidate its potential and inform clinical decision-making.
Spatial and Temporal Variability of Extreme Precipitation Events in the Southeastern United States
Authors: Rahman Mohammad Siddiqur , Senkbeil Jason C. , Keellings David J.
Published: August 2023

Much of the Southeastern United States (SeUS) has experienced an increasing number of extreme precipitation events in recent decades. Characterizing these extreme precipitation events is critical for assessing risk from future hydroclimatic extremes and potential flash flooding. A threshold of one inch per hour (1IPH) was used to indicate an extreme precipitation event. Non-parametric tests were run to identify trends in 1IPH event frequency and locate time series change points. In the last 20 years, 1IPH events increased by 53 percent in the SeUS, and 21/61 stations recorded significant increasing trends. A change point is identified in 15/61 stations. June, July, and August are generally the peak time for 1IPH events, but Florida, Louisiana, and Mississippi recorded longer peak seasons. For the time between events, 17/61 stations recorded significant decreasing trends, implying that 1IPH events are increasing in frequency. Four teleconnection indices were positively correlated with 1IPH events. The SeUS experiences considerable tropical cyclone-induced extreme precipitation, yet only seven percent of 1IPH events overlapped with tropical cyclones. Therefore, the increasing frequency of 1IPH events is likely the result of a combination of baroclinic frontal zones or regional and mesoscale convective features. Causes for the increasing frequency of 1IPH events require further research.
Revisiting Climate-Related Agricultural Losses across South America and Their Future Perspectives
Authors: Gouveia Célia M. , Justino Flávio , Gurjao Carlos , Zita Lormido , Alonso Catarina
Published: August 2023

Climate plays a major role in the spatiotemporal distribution of most agricultural systems, and the economic losses related to climate and weather extremes have escalated significantly in the last decades. South America is one of the most productive agricultural areas of the globe. In recent years, remote sensing data and geographic information systems have been used to improve geo-environmental hazard assessment. However, food security is still highly dependent on small farmer practices that are frequently the most vulnerable to climate extremes. This work reviews climate and weather extremes’ impacts on crop production for South American countries, focusing on the projected ones considering different climate scenarios and countries. A positive trend in the productivity of maize, mainly related to agricultural improvements, was recently observed in Colombia, Ecuador, and Uruguay by up to 200%, as well as in the case of soybean in Bolivia and Uruguay by about 125%. Despite the generalized adverse impacts of climate extremes, results from agrometeorological models generally indicate an increase in crop production in southern regions of Chile (and highlands) and Brazil mainly related to increased temperature. Positive impacts in response to CO2 fertilization are also foreseen in Peru and Brazil (southeast, south, and Minas Gerais); in particular, in Brazil, increases in productivity can be raised by about 40%. The use of double-cropping systems, although with very good results in recent years, may also be at risk in a few decades, mainly due to forecasted precipitation decrease, delay in rainy season onset, and temperature increase. The development of timely early warning systems is imperative to produce technically accurate alerts and the interpretation of the risk assessment based on the link between producers and consumers. Promoting climate index insurance is crucial to build resilient food production, but its implementation should rely on regional or international support systems. Moreover, the implementation of adaptation and mitigation also requires climate-resilient technologies that involve an interdisciplinary approach.
Early Maladaptive Schemas and Schema Modes among People with Histories of Suicidality and the Possibility of a Universal Pattern: A Systematic Review
Authors: Grażka Anna , Strzelecki Dominik
Published: August 2023

Background: The identification of variables affecting suicidality and the search for interventions to reduce suicide risk are priorities among mental health researchers. A promising direction for such research is schema therapy and its two main constructs, i.e., early maladaptive schemas (EMSs) and schema modes. Methods: This systematic review was designed in accordance with the PRISMA guidelines. It summarizes the studies conducted to date that describe the relationship between EMSs and schema modes and measures of suicidality in individuals over the age of 16. Results: The review confirmed that there are many significant associations between EMSs (especially from the Disconnection/Rejection domain) and suicide risk. Although only one study was found that explores the association between schema modes and suicidality, the correlations it identified are also confirmed here. Discussion: The results show the unquestionable importance of EMSs and schema modes in assessing suicide risk. The co-occurrence of these variables represents the first step in further assessing causality and introducing schema therapy techniques into work with patients who are at risk of suicide. This issue requires more extensive experimental research.
MicroRNA-1289 Functions as a Novel Tumor Suppressor in Oral Squamous Cell Carcinoma
Authors: Nakashiro Koh-ichi , Tokuzen Norihiko , Saika Masato , Shirai Hiroyuki , Kuribayashi Nobuyuki , Goda Hiroyuki , Uchida Daisuke
Published: August 2023

Recently, numerous tumor-suppressive microRNAs (TS-miRs) have been identified in human malignancies. Here, we attempted to identify novel TS-miRs in oral squamous cell carcinoma (OSCC). First, we transfected human OSCC cells individually with 968 synthetic miRs mimicking human mature miRs individually, and the growth of these cells was evaluated using the WST-8 assay. Five miR mimics significantly reduced the cell growth rate by less than 30%, and the miR-1289 mimic had the most potent growth inhibitory effect among these miRs. Subsequently, we assessed the in vivo growth-inhibitory effects of miR-1289 using a mouse model. The administration of the miR-1289 mimic–atelocollagen complex significantly reduced the size of subcutaneously xenografted human OSCC tumors. Next, we investigated the expression of miR-1289 in OSCC tissues using reverse transcription–quantitative PCR. The expression level of miR-1289 was significantly lower in OSCC tissues than in the adjacent normal oral mucosa. Furthermore, 15 genes were identified as target genes of miR-1289 via microarray and Ingenuity Pathway Analysis (IPA) microRNA target filtering. Among these genes, the knockdown of magnesium transporter 1 (MAGT1) resulted in the most remarkable cell growth inhibition in human OSCC cells. These results suggested that miR-1289 functions as a novel TS-miR in OSCC and may be a useful therapeutic tool for patients with OSCC.
Colorectal Cancer Surgery: Influence of Psychosocial Factors
Authors: Moldes-Moro Regina , de Dios-Duarte María José
Published: August 2023

(1) Background: In the treatment of colorectal cancer, it is important to consider different psychosocial factors. Our first objective was to measure the levels of perceived stress in subjects diagnosed with colorectal cancer awaiting potentially curative surgery. Also, we aimed to analyse what coping styles these patients used, how they perceived their illness, and the subsequent influence of these factors on their levels of stress. (2) Methods: Stress, coping styles and illness perception were assessed in a sample of 107 patients. The instruments used were the Perceived Stress Scale (PSS-14), the Stress Coping Questionnaire (SCQ) and the Brief Illness Perception Questionnaire (BIPQ-R). (3) Results: Patients using active coping styles have lower levels of perceived stress (p = 0.000; p = 0.002) than patients making use of passive coping styles (p = 0.000; p = 0.032; p = 0.001). A multi-linear regression model found that the perception of illness and the use of the negative approach coping style (p = 0.000; p = 0.001) influence an increase in perceived stress, and that a decrease in stress levels was influenced by the problem solving coping style (p = 0.001). (4) Conclusions: Based on our results, we recommend preventive interventions in care patients undergoing colorectal cancer surgery.
Unraveling the Role of EV-Derived miR-150-5p in Prostate Cancer Metastasis and Its Association with High-Grade Gleason Scores: Implications for Diagnosis
Authors: Cruz-Burgos Marian , Cortés-Ramírez Sergio A. , Losada-García Alberto , Morales-Pacheco Miguel , Martínez-Martínez Eduardo , Morales-Montor Jorge Gustavo , Servín-Haddad Alejandro , Izquierdo-Luna J. Samuel , Rodríguez-Martínez Griselda , Ramos-Godínez María del Pilar , González-Covarrubias Vanessa , Cañavera-Constantino Abraham , González-Ramírez Imelda , Su Boyang , Leong Hon S. , Rodríguez-Dorantes Mauricio
Published: August 2023

Metastasis remains the leading cause of mortality in prostate cancer patients. The presence of tumor cells in lymph nodes is an established prognostic indicator for several cancer types, such as melanoma, breast, oral, pancreatic, and cervical cancers. Emerging evidence highlights the role of microRNAs enclosed within extracellular vesicles as facilitators of molecular communication between tumors and metastatic sites in the lymph nodes. This study aims to investigate the potential diagnostic utility of EV-derived microRNAs in liquid biopsies for prostate cancer. By employing microarrays on paraffin-embedded samples, we characterized the microRNA expression profiles in metastatic lymph nodes, non-metastatic lymph nodes, and primary tumor tissues of prostate cancer. Differential expression of microRNAs was observed in metastatic lymph nodes compared to prostate tumors and non-metastatic lymph node tissues. Three microRNAs (miR-140-3p, miR-150-5p, and miR-23b-3p) were identified as differentially expressed between tissue and plasma samples. Furthermore, we evaluated the expression of these microRNAs in exosomes derived from prostate cancer cells and plasma samples. Intriguingly, high Gleason score samples exhibited the lowest expression of miR-150-5p compared to control samples. Pathway analysis suggested a potential regulatory role for miR-150-5p in the Wnt pathway and bone metastasis. Our findings suggest EV-derived miR-150-5p as a promising diagnostic marker for identifying patients with high-grade Gleason scores and detecting metastasis at an early stage.
Inflammatory Bowel Disease and Colorectal Cancer: Epidemiology, Etiology, Surveillance, and Management
Authors: Sato Yoshihiro , Tsujinaka Shingo , Miura Tomoya , Kitamura Yoh , Suzuki Hideyuki , Shibata Chikashi
Published: August 2023

Patients with inflammatory bowel diseases (IBDs), such as ulcerative colitis and Crohn’s disease, have an increased risk of developing colorectal cancer (CRC). Although advancements in endoscopic imaging techniques, integrated surveillance programs, and improved medical therapies have contributed to a decreased incidence of CRC in patients with IBD, the rate of CRC remains higher in patients with IBD than in individuals without chronic colitis. Patients with IBD-related CRCs exhibit a poorer prognosis than those with sporadic CRCs, owing to their aggressive histological characteristics and lower curative resection rate. In this review, we present an updated overview of the epidemiology, etiology, risk factors, surveillance strategies, treatment recommendations, and prognosis of IBD-related CRCs.
A Statistical Approach to Macrofungal Diversity in a Mediterranean Ecosystem of the Iberian Peninsula Dominated by the Holm Oak (Quercus ilex L. subsp. ballota (Desf.) Samp.)
Authors: Fernández-Ruiz Abel , Vicente-Villardón José Luis , Sánchez-Sánchez José , García-Jiménez Prudencio , Sánchez-Durán Sergio , Rodríguez-de la Cruz David
Published: August 2023

The fungal biodiversity associated with a stable plant community appears to vary from year to year. To analyse the annual behaviour in the formation of fruiting bodies, a statistical study of the data obtained for 4 years (2009–2012) in an apparently undisturbed Mediterranean ecosystem dominated by the holm oak (Quercus ilex L. subsp. ballota (Desf.) Samp.), located in the Midwest of the Iberian Peninsula, was carried out. These data were related to the main meteorological variables. The 150 species collected showed a significant annual, monthly, and weekly difference in their fruiting during the collection period. All this implies a variation in the annual fungal fruiting which can modify the moment of when maximum peaks of fruiting appear, their duration, and the number of species that compose them. In addition, the results make it possible to establish an annual behaviour pattern, with sporocarp formation throughout the year and four fruiting groups (two of them in the dry season). They also allow for inferring a possible response to climate change, with a delay in the fruiting of the autumn-winter group and earlier fruit bearing in the winter-spring group.
Effect of Temperature, Seed Size, Sowing Depth, and Position on Seed Germination and Seedling Growth of Bauhinia retusa Roxb. and Bauhinia variegata L.
Authors: Yadav Neeraj , Khanduri Vinod Prasad , Singh Bhupendra , Dhanai Chatar Singh , Riyal Manoj Kumar , Rawat Deepa , Ahmad Taufiq , Kumar Munesh
Published: August 2023

In urban forestry plantations are implemented in different cities of the world for social and environmental benefits. Bauhinia retusa and Bauhinia variegata are important species and to be used as large-scale plantation programs in urban forestry which might solve or mitigate urban, social, and environmental issues such as improving the physical & mental health of residents, food and nutrition security, increasing urban biodiversity, cooling the neighboring, preventing soil erosion, flooding, and mitigating greenhouse gas emissions and air pollution. The present study was conducted with the aim of producing quality planting material for B. retusa and B. variegata in the nursery for afforestation programs. Seeds of B. retusa and B. variegata were collected from the natural habitats to assess seed germination and seedling growth. Seeds were stored in different types of containers at room temperature and later on exposed to 15, 20, and 25 °C in seed germinator. Seeds were further sown in polythene bags according to the seed size, seed coat color, seed sowing depth, the orientation of seeds, and the result of the emergence of seedlings, their growth, and biomass were estimated. A two-way analysis of variance was calculated to estimate the variation among the studied parameters. Results revealed that a constant 25 °C temperature was considered best for seed germination of both the Bauhinia species. Polybags were found the most suitable for storing the Bauhinia seeds among the storage containers. The seedling emergence and growth were maximum in yellow color and large seeds. In B. retusa, seedling emergence, and growths were the maximum in seeds sown at a horizontal position and in B. variegata at an upright position. Seedling emergence, length, and biomass were recorded the maximum when seeds of B. retusa were sown at 4 cm depth and B. variegata seeds were sown at 2 cm depth. The study recommends that the yellow color seed that has to be sown at 2 cm to 4 cm depth with upright and horizontal positions is considered best for the production of quality planting stock of both studied Bauhinia species.
Liver Involvement in Patients with Rare MBOAT7 Variants and Intellectual Disability: A Case Report and Literature Review
Authors: Ronzoni Luisa , Mureddu Matteo , Malvestiti Francesco , Moretti Vittoria , Bianco Cristiana , Periti Giulia , Baldassarri Margherita , Ariani Francesca , Carrer Anna , Pelusi Serena , Renieri Alessandra , Prati Daniele , Valenti Luca
Published: August 2023

The membrane-bound O-acyltransferase domain-containing 7 (MBOAT7) protein is an acyltransferase catalyzing arachidonic acid incorporation into lysophosphatidylinositol. Patients with rare, biallelic loss-of-function variants of the MBOAT7 gene display intellectual disability with neurodevelopmental defects. The rs641738 inherited variant associated with reduced hepatic MBOAT7 expression has been linked to steatotic liver disease susceptibility. However, the impact of biallelic loss-of-function MBOAT7 variants on liver disease is not known. We report on a 2-year-old girl with MBOAT7-related intellectual disability and steatotic liver disease, confirming that MBOAT7 loss-of-function predisposes to liver disease.
Exploring the Binding Affinity of the ARR2 GARP DNA Binding Domain via Comparative Methods
Authors: Rieger Janine , Fitz Michael , Fischer Stefan Markus , Wallmeroth Niklas , Flores-Romero Hector , Fischer Nina Monika , Brand Luise Helene , García-Sáez Ana J. , Berendzen Kenneth Wayne , Mira-Rodado Virtudes
Published: August 2023

Plants have evolved signaling mechanisms such as the multi-step phosphorelay (MSP) to respond to different internal and external stimuli. MSP responses often result in gene transcription regulation that is modulated through transcription factors such as B-type Arabidopsis response regulator (ARR) proteins. Among these proteins, ARR2 is a key component that is expressed ubiquitously and is involved in many aspects of plant development. Although it has been noted that B-type ARRs bind to their cognate genes through a DNA-binding domain termed the GARP domain, little is known about the structure and function of this type of DNA-binding domain; thus, how ARRs bind to DNA at a structural level is still poorly understood. In order to understand how the MSP functions in planta, it is crucial to unravel both the kinetics as well as the structural identity of the components involved in such interactions. For this reason, this work focusses on resolving how the GARP domain of ARR2 (GARP2) binds to the promoter region of ARR5, one of its native target genes in cytokinin signaling. We have established that GARP2 specifically binds to the ARR5 promoter with three different bi-molecular interaction systems—qDPI-ELISA, FCS, and MST—and we also determined the KD of this interaction. In addition, structural modeling of the GARP2 domain confirms that GARP2 entails a HTH motif, and that protein–DNA interaction most likely occurs via the α3-helix and the N-terminal arm of this domain since mutations in this region hinder ARR2’s ability to activate transcription.
Characterizing the Contribution of Indoor Residential Phthalate and Phthalate Alternative Dust Concentrations to Internal Dose in the US General Population: An Updated Systematic Review and Meta-Analysis
Authors: Milton Sashoy G. , Tejiram Rachel A. , Joglekar Rashmi , Hoffman Kate
Published: August 2023

Diet is the primary exposure pathway for phthalates, but relative contributions of other exposure sources are not well characterized. This study quantifies the relative contribution of indoor residential dust phthalate and phthalate alternative concentrations to total internal dose estimated from the National Health and Nutrition Examination Survey (NHANES) urinary metabolite concentrations. Specifically, median phthalate and phthalate alternative concentrations measured in residential dust were determined by updating a pre-existing systematic review and meta-analysis published in 2015 and the attributable internal dose was estimated using intake and reverse dosimetry models. Employing a predetermined search strategy, 12 studies published between January 2000 and April 2022 from Web of Science and PubMed measuring phthalates and phthalate alternatives in residential dust were identified. From the data extracted, it was estimated that dust contributed more significantly to the internal dose of low-molecular weight chemicals such as DEP and BBP when compared to high-molecular weight chemicals such as DEHTP. Additionally, findings showed that the chemical profile of residential dust is changing temporally with more phthalate alternatives being detected in the indoor environment. Future studies should seek to characterize the contribution of dust to an overall phthalate and phthalate alternative intake for individuals who have higher than normal exposures.
Boldine Alters Serum Lipidomic Signatures after Acute Spinal Cord Transection in Male Mice
Authors: Graham Zachary A. , Siedlik Jacob A. , Toro Carlos A. , Harlow Lauren , Cardozo Christopher P.
Published: August 2023

Traumatic spinal cord injury (SCI) results in wide-ranging cellular and systemic dysfunction in the acute and chronic time frames after the injury. Chronic SCI has well-described secondary medical consequences while acute SCI has unique metabolic challenges as a result of physical trauma, in-patient recovery and other post-operative outcomes. Here, we used high resolution mass spectrometry approaches to describe the circulating lipidomic and metabolomic signatures using blood serum from mice 7 d after a complete SCI. Additionally, we probed whether the aporphine alkaloid, boldine, was able to prevent SCI-induced changes observed using these ‘omics platforms’. We found that SCI resulted in large-scale changes to the circulating lipidome but minimal changes in the metabolome, with boldine able to reverse or attenuate SCI-induced changes in the abundance of 50 lipids. Multiomic integration using xMWAS demonstrated unique network structures and community memberships across the groups.