bluuebunny/crossref_metadata_2025 huggingface dataset:
Didática da história e a competência de atribuição de sentido: um estudo a partir da metodologia da educação histórica
Authors: Cainelli Marlene
Published: May 2019

RESUMO Neste artigo apresentamos como, a partir da metodologia de investigação com pesquisas interculturais baseadas na aplicação de questionários fechados e/ou abertos (BORRIES & ANGVIK,1997), com estudos exploratórios realizados pelo projeto, com professores de quatro Estados do Brasil: Paraná, São Paulo, Goiás e Bahia, nos foi possível chegar aos resultados que apresentamos neste estudo, que procurou entender quais são as carências de orientação que indicam os caminhos que os professores seguem que determinam suas escolhas de conteúdo historiográfico e do método de ensino. Quais demandas do passado? Quais experiências e sentimentos? Analisamos o diálogo estabelecido pelos professores com a concepção de história a partir de uma narrativa apresentada em questionário fechado sobre o descobrimento do Brasil. Nesta pesquisa trabalhamos com Peter Seixas (2016) com o objetivo de perceber qual o lugar da educação histórica na produção de conhecimento na escola e na formação do pensamento histórico criando uma estratégia de tratamento dos dados em conjunto entre as concepções teóricas e a fala dos sujeitos analisados.
Métodos, instrumentos y procedimientos para conocer cómo se evalúan las competencias históricas
Authors: Miralles Pedro , Monteagudo-Fernández José
Published: May 2019

RESUMEN Este artículo intenta explicar las características de la evaluación en la materia de Historia en lo referente a la tipología de preguntas, capacidades cognitivas, competencias históricas y tipos de contenidos que los exámenes y libros de texto demandan al alumnado de Educación Secundaria. Para ello se ha adoptado un diseño metodológico mixto, defendido en diversas investigaciones desde el área de didáctica de las ciencias sociales (BARCA, 2005; BARTON, 2005, 2012; GRANT, 2001; PRATS, 2002). Esta complementariedad de los enfoques cuantitativos y cualitativos creemos que nos permite acercarnos mejor a un fenómeno tan complejo como es la evaluación. Para el análisis cuantitativo se ha utilizado el paquete estadístico SPSS, en todo lo referente al estudio de frecuencias, medias, porcentajes, chi cuadrado, tablas de contingencia y análisis de la dependencia entre las variables. Por su parte, el tratamiento cualitativo se ha llevado a cabo a través de un análisis de cómo se ha construido la narrativa histórica en los exámenes y los libros de texto, a través de la frecuencia y repetición de hechos, conceptos, fechas y personajes. Además, hemos utilizado el programa Access para el procesamiento y vinculación de datos mediante la creación de tablas relacionales.
Escolha das Energias de Soldagem para Aplicação na Técnica da Dupla Camada na Soldagem do Aço ASTM 131 Grau AH 36
Authors: Cozza Leandro Macedo , Medeiros Jorge Luis Braz , Biehl Luciano Volcanoglo , Souza José de , Ferreira Filho Demostenes
Published: May 2019

Resumo Durante o processo de soldagem o aço microligado ASTM A131 Grau AH36 pode apresentar defeitos oriundos dos ciclos térmicos (distorções) que demandarão procedimentos onerosos como o tratamento térmico pós-soldagem. Somando-se a isto, este aço não é indicado a sofrer normalização, normalização seguida por revenimento, ou por têmpera e revenimento. Neste caso, a técnica da dupla camada, que se baseia na deposição controlada dos passes da primeira e segunda camada através da escolha apropriada da relação de energias de soldagem, surge como alternativa. Diante disto, este trabalho teve por objetivo encontrar a relação de energia de soldagem que satisfizesse os critérios de microdureza e macrografia. Para isto foram realizadas soldagens preliminares para cada energia e após isto a comprovação com a soldagem de dupla camada em chapa plana. Os resultados indicaram que as relações de energias de soldagem 5/14 e 5/17 kJ.cm-1 foram aprovadas, porém a energia de 5/14 kJ.cm-1 foi a que apresentou o maior afastamento no critério baseado na microdureza. A soldagem com dupla camada comprovou que a relação de energia encontrada foi adequada apresentando microdurezas com valores inferiores ao limite da zona endurecida (300 HV0,1) demonstrando a possibilidade de revenimento ocasionado pela sobreposição dos passes.
Butterflies (Lepidoptera: Papilionoidea) from the campos rupestres of Serra de São José, Minas Gerais, Brazil
Authors: Henriques Nathália Ribeiro , Beirão Marina do Vale , Brasil Ello , Cornelissen Tatiana
Published: May 2019

Abstract We provide the first inventory of butterfly species (Lepidoptera: Papilionoidea) in Serra de São José, Minas Gerais, Brazil. Serra de São José has elevations ranging from 800 m to 1,400 m above sea level; the butterflies were sampled using traps and entomological nets in seven plots along the altitudinal gradient. We recorded 647 butterflies belonging to 112 species and six families. We also recorded one threatened species and three endemic species for the Cerrado domain, which suggests that Serra de São José is an important refuge for butterfly conservation.
Winged ants (Hymenoptera: Formicidae) presence in twigs on the leaf litter of Atlantic Forest
Authors: Fernandes Tae Tanaami , Silva Rogério R. , Souza-Campana Débora Rodrigues de , Silva Otávio Guilherme Morais da , Morini Maria Santina de Castro
Published: May 2019

Abstract: In the leaf litter, ants have various nesting resources available, such as live or dead trunks, twigs, leaves, fruits and seeds. On the twigs, there are adults and immature individuals, but also the queen and winged. The production of wings requires time and energy from the colony. The objective of this study was to investigate the presence of winged in ant colonies in twigs on the leaf litter. Our prediction is that the richness and abundance of winged in twigs are the greatest in rainy months. We collected all twigs with ants in 552 plots with 16 m2, totaling 8,832 m2 of leaf litter, in areas located in the Brazilian Atlantic Domain. We compared the species richness and the number of colonies with and without winged, as well as the number of winged over a year. In total, we collected 1,521 twigs with colonies belonging to 92 species of ants. The rate of twigs with winged was low, about 12%. In colonies with winged, the total number of twigs, species and amount of winged does not differ between the months considered dry and rainy. The majority of winged species are leaf litter dwellers, such as Linepithema neotropicum, recorded with the highest amount of winged irrespective of the period. Arboreal species colonized 15% of the twigs and, in 1/3 of these species, winged were recorded as part of the composition of the colony. Although winged represent a small percentage of the colony in twigs, our results indicate that this feature is important for the life cycle of 44% of the species that occupy twigs, considering that winged are fundamental for the dispersion of the colony.
Landscapes of memory: the first visual images of the Bororo of Central Brazil
Authors: Cunha Edgar Teodoro da , Novaes Sylvia Caiuby
Published: May 2019

Abstract The opportunity of exhibiting in a Bororo village in Central Brazil the film Matto Grosso, the Great Brazilian Wilderness (1931), and translating into Portuguese this typical travelogue and widely considered to be the first documentary with synchronized sound, led the authors to analyze visual images on the Bororo society made in the first decades of the XX century. The article focuses on how visual images - films and photographs - frequently show the intentions of the author and on the other hand may enhance memories and create a particular relationship with the past and history of a people.
The Governance of Safeguarding. Comments on Article 2.3 of UNESCO’s Convention for the Safeguarding of Intangible Cultural Heritage
Authors: Arantes Antonio
Published: May 2019

Abstract Reflexions on the key dispositif1 adopted by Unesco’s Convention on the Safeguarding of Intangible Cultural Heritage (Article 2.3). In these Comments, I initially situate the notion of ‘safeguarding’ in the context of transformations of other preservation instruments which it dialogues and to whose semantic field it belongs. Challenges to its implementation and possibilities opened by this treaty for the protection of what has been designated as ‘folklore and traditional (and popular) culture’2 are addressed. After offering an interpretation of its textual meaning in the Convention, I seek to explore how this device is articulated to others in this Convention, and to reflect on its possible practical reach.
NURSING PROTOCOL FOR REMOTE MONITORING OF WOMEN WITH EXCESSIVE WEIGHT
Authors: Palmeira Catia Suely , Mussi Fernanda Carneiro , Ramos Giulia Araújo , Jesus Natália Vieira de , Macedo Tassia Teles Santana de , Dal Sasso Grace Teresinha Marcon
Published: May 2019

ABSTRACT Objective: to describe the construction and implementation of the nursing protocol for remote monitoring of overweight women. Method: report of experience with 50 women who participated in an intervention study developed in a reference outpatient clinic for obesity in Salvador, Brazil. The steps involved in the development of the protocol consisted of structuring the theoretical content and defining the form of operationalization. Results: the definition of the theoretical content of the protocol was guided by the main guides and guidelines of the Ministry of Health and Scientific Societies and discussion with specialists. A thematic weekly script was developed for phone calls, including content on obesity, its causes and complications, feeding and practicing physical activity for weight control. The protocol was performed in ten weeks, with scheduled weekly connections and duration of five minutes. To guide the team in the operation of telephone calls, a Telephone Call Guide was developed. The information was offered in a flexible way, providing moments for the participant to clarify doubts and to add information, being observed the understanding of the contents. Women noted that monitoring, as well as guiding how they could take better care of themselves, helped to avoid giving up treatment and solving problems with health consultations and procedures. Conclusion: the use of remote nursing monitoring focused on educational actions, has proved to be a tool to support the care of overweight women.
Interesses capitalistas e desafios para a efetivação dos direitos sociais: ataques e regressões
Authors: Lage Lais do Nascimento Vidal
Published: May 2019

Resumo Este artigo traz uma reflexão sobre as diversas ofensivas capitalistas nas conquistas civilizatórias alcançadas ao longo da história na sociedade brasileira, com apontamentos sobre o desenvolvimento do capitalismo no mundo e seus rebatimentos no Brasil levando em conta seu contexto sócio-histórico para compreender a formulação e implementação da política social, enquanto uma representação e defesa dos direitos sociais que sofrem a todo tempo, principalmente nos últimos anos a partir do neoliberalismo, regressões e contrarreformas.
Intervención social y los giros en la política
Authors: Pieruzzini Rosana , Salera Maricel , Frank Judit Evelyn
Published: May 2019

Resumen Este artículo se basa en las indagaciones realizadas en el marco del proyecto de investigación en torno a la intervención social y su relación con los procesos de territorialidad, desterritorialidad y politicidad en la ciudad de Paraná1 (Provincia de Entre Ríos, Argentina). Se analiza la intervención social desde una perspectiva historiográfica, tomando documentos e informes cuantitativos publicados por instituciones académicas y otros centros de estudio o estadística. El análisis se propone con un diseño cualitativo, esbozando la operacionalización de los conceptos claves en los datos empíricos que ofrece el caso argentino desde el advenimiento de la democracia hasta el mes de diciembre del año 2017. La presente versión fue revisada y ampliada en función de los conceptos y los acontecimientos políticos y sociales en el transcurrir los nuevos modelos de políticas que se imponen desde 2015 en la mayoría de los países de América Latina, nos ocupan puntualmente las transformaciones en materia de Políticas Sociales que han tenido lugar en Argentina.
Encarceramento juvenil: o legado histórico de seletividade e criminalização da pobreza
Authors: Bonalume Bruna Carolina , Jacinto Adriana Giaqueto
Published: May 2019

Resumo O presente artigo tem como objetivo propor reflexões acerca do encarceramento juvenil, bem como sobre a maneira como a violência atravessa o cotidiano dessa população. Essa discussão está pautada no reconhecimento da seletividade da justiça frente a uma sociedade marcada profundamente pela desigualdade de gênero, classe social e raça/etnia. Para adentrar nesse cenário serão tecidas discussões construídas na perspectiva de contextualizar a questão social, suas expressões e o modo como o Estado historicamente tem respondido, ou seja, com coerção e consenso, o que revela o quanto o enfrentamento dessa realidade tem suas raízes fundadas em uma sociedade desigual e que sustenta uma lógica de criminalização da pobreza.
Emancipação política e a luta pelo direito à cidade
Authors: Lopes Ada Kallyne Sousa
Published: May 2019

Resumo O presente texto busca apreender o solo histórico onde se processa a luta pelo direito à cidade no Brasil e os limites postos por estas determinações histórico-estruturais à emancipação política da cidade. Trata-se de um trabalho de natureza teórica que, por meio de uma revisão bibliográfica, procurou construir uma interlocução com importantes teóricos da formação social brasileira, a fim de elucidar os entraves e os desafios à plena materialização do direito à cidade em um país de capitalismo de natureza dependente, como o Brasil. Como resultado compreendemos que a luta anticapitalista torna-se vital para a materialização real de cidades mais democráticas, justas e sustentáveis, uma vez que a luta urbana deve ter como horizonte, além da conquista de sua emancipação política - fundamental em sociedades desiguais como a brasileira - a adoção de uma direção crítica socialmente referenciada nas necessidades da classe trabalhadora para a construção de uma nova ordem societária.
Trabajo, derechos sociales y protección social en Argentina de la reconstrucción neoliberal
Authors: Hopp Malena Victoria , Lijterman Eliana
Published: May 2019

Resumen El artículo analiza la reorganización de la protección social en Argentina, iniciada a fines de 2015. Toma como analizadores la clausura de políticas centrales de la estrategia de inclusión por el trabajo que caracterizó al ciclo político previo: la moratoria previsional y la promoción de cooperativas de trabajo. En base al análisis documental se examinan los modos en que se reconstruyeron los sentidos del trabajo y el reconocimiento de derechos en la definición de los problemas sociales y las estrategias de intervención estatal para abordarlos. El análisis realizado muestra que los procesos de contrarreforma neoliberal en curso confluyen en una redefinición del valor social del trabajo y el horizonte de integración social y económica de las poblaciones vulnerables. Estos nuevos sentidos parten de una interpretación restringida y mercantilizada de los derechos sociales y del trabajo que tienen como efectos la asistencialización e individualización de las protecciones sociales.
Expropriação do funcionalismo público: o gerencialismo como projeto
Authors: Reis Tiago Siqueira
Published: May 2019

Resumo O presente trabalho tem por objetivo compreender o projeto de expropriação social do aparelho do Estado brasileiro a partir da análise da ideia de administração gerencial relativa às instituições públicas. Delimitamos nossa investigação entre os anos de 1995 e 2002, quando Fernando Henrique Cardoso esteve à frente do governo no Brasil, levando em consideração o projeto de Reforma do Estado. Portanto, pretendemos perceber a partir da noção de capital-imperialismo quais são as bases teóricas que sustentam a ideia de reforma gerencial protagonizada pelo governo Cardoso.
ARBUSCULAR MYCORRHIZAL FUNGI AND PHOSPHORUS DOSES ON COFFEE GROWTH UNDER A NON-STERILE SOIL
Authors: Moreira Samuel Dias , França André Cabral , Grazziotti Paulo Henrique , Leal Felipe Douglas Soares , Silva Enilson de Barros
Published: May 2019

ABSTRACT The use of mycorrhiza is a promising biotechnology that makes coffee cultivation sustainable in the use of fertilizers. In this sense, plant growth and phosphorus contents were assessed in coffee inoculated with Rhizophagus clarus, Claroideoglomus etunicatum and Dentiscutata heterogama in soil fertilized with 0, 0.74, 1.48 and 2.96 g P2O5 kg-1. The experimental design was a randomized block design in a 4 × 4 factorial scheme with four replications. The mycorrhizal seedlings were previously produced and later transplanted to pots, simulating the field planting. The assessments were performed 150 days after transplanting. All variables were influenced by inoculation, but the effects were dependent on P doses. P doses higher than 50% of the recommended dose (0.74 g P2O5 kg-1) decreased the benefit of mycorrhiza. The fungi Rhizophagus clarus and Claroideoglomus etunicatum promoted a higher growth of coffee plants when compared to non-inoculated plants in non-sterilized soils and with a reduction in the applied P.
PROBABILITY DISTRIBUTION FUNCTIONS APPLIED IN THE WATER REQUIREMENT ESTIMATES IN IRRIGATION PROJECTS
Authors: Fernandes Rafael Dreux Miranda , José Jefferson vieira , Wolff Wagner , Costa Jéfferson de oliveira , Folegatti Marcos Vinícius
Published: May 2019

ABSTRACT Spain contains a third the entire irrigated area of Europe, accounting for 15% of the cultivated area of the country and almost 60% of the national agricultural production. Knowledge of the spatial and temporal variability of reference evapotranspiration (ETo) and the probabilistic theory of extreme events is crucial for the elaboration of sustainable irrigation projects. The objective of this work was to define the frequency distribution that best describes ETo for the design of irrigation systems in the region of Andalusia. We used ETo data for the period 2001 to 2015 from 56 meteorological stations. The values were accumulated over three consecutive days. For all accumulated periods, nine probability distributions were adjusted. The probability distribution that best described ETo for the design of irrigation systems in the region was the Gumbel II distribution. The maximum daily ETo to be considered in irrigation projects in this region is, on average, 10 mm. The accumulated ETo for periods of 5, 10, and 30 days that should be considered are, on average, 42 mm, 78, mm and 224 mm, respectively.
SOIL CHEMICAL PROPERTIES IN BANANA CROPS FERTIGATED WITH TREATED WASTEWATER
Authors: Alves Pablo Fernando Santos , Santos Silvânio Rodrigues dos , Kondo Marcos Koiti , Pegoraro Rodinei Facco , Portugal Arley Figueiredo
Published: May 2019

ABSTRACT Determining the effects of using wastewater as fertilizer on soil chemical properties allows a safe reuse of this effluent in agriculture. This study evaluated the effects of fertigation with tertiary treated wastewater (TTW) from the Janaúba sewage treatment plant on chemical properties of a Latosol (Oxisol) with banana crops of the Prata-Anã cultivar in the semiarid region of Brazil. A randomized complete block design with four replications was used to test four TTW rates (70%, 130%, 170%, and 200% of the limit of 150 kg ha-1 year-1 of Na that can be applied to the soil) and compare them to a control without TTW. Soil samples from the 0.0-0.2, 0.2-0.4, 0.4-0.6, and 0.6-0.8 m layers were collected at the end of the first crop cycle to evaluate soil chemical properties-pH, soil organic matter (SOM), P, K, Na, Ca, Mg, Al, potential acidity (H+Al), base saturation, B, Cu, Fe, Mn, Zn, remaining P, and electrical conductivity (EC). The use of TTW increases soil pH and decreases exchangeable Al content, thus, reduces the need for liming. However, Na contents increased faster than EC in the soil, indicating that the use of TTW tends to alter soil physical properties over time. The use of TTW had no effect on the soil OM, P, Ca, Mg, and micronutrients contents, potential acidity, and base saturation. The changes in soil chemical attributes observed at the end of the first crop cycle were not limiting to the banana crop.
METHIONINE PLUS CYSTINE TO LYSINE RATIO IN DIETS FOR TAMBAQUI JUVENILES
Authors: Souza Flávio Oliveira , Bomfim Marcos Antonio Delmondes , Ribeiro Felipe Barbosa , Lanna Eduardo Arruda Teixeira , Sousa Thalles José Rêgo de , Costa Dayana da Conceição da
Published: May 2019

ABSTRACT The objective of this study was to determine the appropriate ratio of methionine plus cystine to lysine in rations for tambaqui (Colossoma macropomum) juveniles, with initial weights of 0.28 ± 0.08 g and 0.94 ± 0.33 g. A randomized block design consisting of six treatments, five replicates in two blocks, and 20 fish per replicate was implemented. Six isolisinic (1.45%), isoenergetic, isophosphoric, and isocalcic diets, containing different ratios of methionine plus cystine to digestible lysine (50%, 55%, 60%, 65%, 70%, and 75%) were used. Performance and feed efficiency variables, including body composition (moisture, protein, and fat), daily protein deposition rate, daily fat deposition rate, and nitrogen retention efficiency, were evaluated. The specific growth rate and feed conversion presented quadratic effects, with maximum effects from the digestible methionine plus cystine to lysine ratio of 64.4% and 64.8%, respectively. The increase in digestible methionine plus cystine intake resulted in a linear reduction in body fat content with the elevation of the digestible methionine plus cystine to lysine ratio. The digestible methionine plus cystine to lysine ratio recommended in rations for tambaqui fingerlings is 64.8%.
REACTION OF Crambe abyssinica TO Meloidogyne javanica AND M. incognita RACE 3
Authors: Souza Rafael Assis de , Ribeiro Regina Cássia Ferreira , Rocha Leandro de Souza , Xavier Adelica Aparecida , Martins Isac Pereira Soares , Silva Fabíola de Jesus
Published: May 2019

ABSTRACT Several oilseed plants have been researched for biodiesel production in Brazil, and Crambe abyssinica Hochst is one of the most promising species. The effect of the phytonematodes M. incognita race 3 and Meloidogyne javanica on C. abyssinica plants was evaluated in greenhouse experiments. A randomized block design with five nematode inoculum levels (350, 700, 1400, 2800, and 5600 eggs) and ten replications was used. C. abyssinica plants without inoculation were used as controls, and the viability of the nematodes used was tested in tomato plants inoculated with 2000 eggs. The plots consisted of pots containing one plant. The number of root knots (NG), number of egg masses (NEM), and number of eggs + second-stage juveniles (NEJ2) per root system, number of J2 per 200 cm3 of soil (NJ2), and shoot dry weight (SDW) of the inoculated C. abyssinica plants were evaluated at 45 days after inoculation. The reproduction factor (RF) of the nematodes was also evaluated. The resistance of the plants to the phytonematodes was classified using the RF criterion. Crambe abyssinica plants are susceptible to the different inoculum levels of Meloidogyne javanica and M. incognita used, and the data of all nematode variables (NG, NEM, NEJ2, NJ2, and RF) were fitted by linear models.
CULTURAL ADAPTATION AND RELIABILITY ANALYSIS OF THE EARLY CLINICAL ASSESSMENT OF BALANCE
Authors: Gontijo Ana Paula Bensemann , Starling Juliana Maria Pimenta , Oliveira Graziela Dulce , Meier Debora , Mancini Marisa Cotta
Published: May 2019

ABSTRACT Objective: To translate the Early Clinical Assessment of Balance (ECAB), an assessment scale developed specifically for children and adolescents with cerebral palsy into Brazilian Portuguese, evaluate semantic, idiomatic, experiential and conceptual equivalences, and to examine the face validity and the reliability within and between examiners of the Brazilian version. Methods: The following steps were done: translation by two independent translators; synthesis of translations; back translation into English; analysis of back-translations by a multidisciplinary committee and the author of the test to develop the final version of the test; test application training; administration of the translated version of ECAB (videotaped) in 60 children and adolescents with cerebral palsy; intra and inter-examiner reliability assessment. Reability was assessed by intraclass correlation coefficient (CCI). Results: The discrepancies found were related mainly to semantic equivalence and, therefore, there was no need to make cultural adaptations in any of the 13 items on the scale. The rate of agreement was greater than 90% and the reliability of the ECAB-Portuguese total score was excellent both for the intra-rater test (CCI=1.00) and for the inter-rater test (CCI=0.998). Likewise, the reliability evaluation of each of the scale items was also excellent. Conclusions: The translated version of the ECAB into Portuguese provides a tool for the evaluation of the specific balance for children and adolescents with cerebral palsy with different levels of functioning.
CASE SERIES OF PATIENTS UNDER BIWEEKLY TREATMENT WITH LARONIDASE: A REPORT OF A SINGLE CENTER EXPERIENCE
Authors: Kyosen Sandra Obikawa , Toma Leny , Nader Helena Bonciani , Braga Marion Coting , Pereira Vanessa Gonçalves , Canossa Sueli , Pesquero João Bosco , D’Almeida Vânia , Martins Ana Maria
Published: May 2019

ABSTRACT Objective: To report the stabilization of urinary glycosaminoglicans (GAG) excretion and clinical improvements in patients with mucopolysaccharidosis type I (MPS I) under an alternative dose regimen of laronidase of 1.2 mg/kg every other week. Methods: We participated in a dose-optimization trial for laronidase in MPS-I patients using four alternative regimens: 0.58 mg/kg every week, 1.2 mg/kg every two weeks, 1.2 mg/kg every week and 1.8 mg/kg every other week (EOW). After the trial ended, the patients resumed the recommended dose and regimen of 0.58 mg/kg every week. Under this regimen, some patients presented difficulties in venous access and were unable to commute weekly to the treatment center. Therefore, we used an alternative regimen that consisted of 1.2 mg/kg EOW in eight patients. A retrospective study of medical records of MPS-I patients who underwent both enzyme replacement therapy (ERT) regimens, of 0.58 mg/kg every week and 1.2 mg/kg EOW, was done. Results: Patients remained clinically stable under the alternative regimen, did not present elevation of urinary GAG nor any adverse event. Conclusions: The switch of dose regimen to 1.2 mg/kg EOW of laronidase was safe, and did not cause any clinical worsening in patients who had been previously under standard dose ERT.
Aplicações dos potenciais evocados miogênicos vestibulares: revisão sistemática de literatura
Authors: Silva Tatiana Rocha , Santos Marco Aurélio Rocha , Resende Luciana Macedo de , Labanca Ludimila , Caporali Júlia Fonseca de Morais , Sousa Marjore Rhaissa de , Gonçalves Denise Utsch
Published: May 2019

RESUMO Objetivos Revisar a literatura científica sobre as principais técnicas usadas para gerar o potencial evocado miogênico vestibular (VEMP) e suas aplicações clínicas. Estratégia de pesquisa Os artigos que descrevem os métodos de registro e as aplicações do VEMP foram localizados nas bases de dados PubMed, Web of Science, MEDLINE, Scopus, LILACS e SciELO. O levantamento realizado limitou-se aos artigos publicados nos idiomas Inglês, Português e Espanhol, entre janeiro de 2012 e maio de 2018. Critérios de seleção Artigos sobre os aspectos técnicos para a realização do VEMP ocular, cervical ou do músculo sóleo, com estimulação auditiva ou galvânica e artigos sobre as aplicações clínicas do VEMP foram incluídos; artigos repetidos nas bases de dados, artigos de revisão de literatura, relato de casos, cartas e editoriais foram excluídos. Resultados A estratégia de busca resultou na seleção de 28 artigos. Os estudos evidenciaram três métodos de registro do VEMP: cervical, ocular e no músculo sóleo. As aplicações clínicas do VEMP incluíram doença de Ménière, neurite vestibular, síndrome da deiscência do canal semicircular superior, doença de Parkinson, lesões centrais isquêmicas e mielopatias motoras. Conclusão Independentemente da técnica de registro, o VEMP mostrou-se útil como ferramenta complementar para o diagnóstico de doenças vestibulares periféricas e centrais.
Efeitos do uso de diferentes tecnologias educacionais na aprendizagem conceitual sobre o sistema miofuncional orofacial
Authors: Rondon-Melo Silmara , Andrade Claudia Regina Furquim de
Published: May 2019

RESUMO Objetivo Comparar três métodos de aprendizagem sobre anatomia e fisiologia do sistema miofuncional orofacial, sendo dois interativos (uso de softwares educacionais) e um tradicional, quanto à aprendizagem conceitual de estudantes de graduação em Fonoaudiologia. Métodos Participaram 36 estudantes do segundo ano, alocados randomicamente em grupos: Grupo 1 (G1) – método interativo com jogo computacional 2D (n=12); Grupo 2 (G2) – método interativo com modelo computacional 3D (n=12); Grupo 3 (G3) – método tradicional (textos e figuras 2D) (n=12). Os métodos de aprendizagem foram aplicados durante estudo complementar, por sete semanas, após aula expositiva. Foi realizada uma avaliação de conhecimento antes da aplicação dos métodos de aprendizagem, imediatamente após e seis meses depois da conclusão, e o desempenho dos grupos, nos três momentos, foi comparado. Os dados foram analisados no software SPSS, versão 21 (nível de significância de 5%). Resultados Predominaram sujeitos do gênero feminino, com média de idade de 22,0 (±4,7) anos (F2,33=60,72, p=0,260). Os resultados indicaram que, no G1, apenas o pré-teste diferiu do pós-teste, enquanto no G2 e no G3 o pré-teste diferiu do pós-teste e do pós-teste tardio. Ao verificar a interação entre o desempenho dos grupos e os momentos de avaliação, observou-se que os resultados do GI foram inferiores aos dos demais grupos (F2,22=722,30 p<0,001). Conclusão O uso de modelo computacional 3D foi comparável ao do método tradicional para a aprendizagem conceitual e retenção de conhecimento, em curto e longo prazo, sendo ambos mais eficazes do que o uso de jogo computacional 2D.
Analysis of DLL3 and ASCL1 in Surgically Resected Small Cell Lung Cancer (HOT1702)
Authors: Furuta Megumi , Sakakibara-Konishi Jun , Kikuchi Hajime , Yokouchi Hiroshi , Nishihara Hiroshi , Minemura Hiroyuki , Harada Masao , Yamazaki Shigeo , Akie Kenji , Fujita Yuka , Takamura Kei , Kojima Tetsuya , Harada Toshiyuki , Minami Yoshinori , Watanabe Naomi , Oizumi Satoshi , Suzuki Hiroyuki , Nishimura Masaharu , Dosaka-Akita Hirotoshi , Isobe Hiroshi ,   on behalf of Hokkaido Lung Cancer Clinical Study Group
Published: May 2019

Background

Delta-like protein 3 (DLL3) is a Notch ligand that has an important role in the tumorigenesis of small cell lung cancer (SCLC). Recently, rovalpituzumab tesirine (Rova-T), a DLL3-targeted antibody-drug conjugate, has been developed for treating SCLC. DLL3 is a transcriptional target of the achaete-scute homolog-1 (ASCL1) transcription factor, which is involved in pulmonary neuroendocrine cell development. However, the relationship between DLL3 and/or ASCL1 expression and the clinical features of SCLC remains unknown, especially for early-stage resected SCLC. This study aimed to investigate the expression of DLL3 and ASCL1 in resected SCLC samples using immunohistochemical analysis.

Materials and Methods

We collected 95 surgically resected SCLC samples, which were formalin fixed and paraffin embedded. Immunohistochemistry staining was performed to investigate the correlation between the expression of either DLL3 or ASCL1 and clinicopathological features of study patients.

Results

Seventy-seven (83%) of 93 immunohistochemically evaluable samples were positive for DLL3 (expression in ≥1% of tumor cells), and DLL3-high expression (≥75%) was observed in 44 samples (47%). Sixty-one (64%) of 95 samples were positive for ASCL1 (expression in ≥5% of tumor cells). A positive correlation was observed between DLL3 and ASCL1 expression. DLL3 and ASCL1 expression were not associated with survival in SCLC patients. DLL3 was more prevalent in patients with advanced clinical disease.

Conclusion

DLL3 and ASCL1 were highly expressed in patients with surgically resected SCLC. DLL3 and ASCL1 may be targets for the treatment of SCLC.

Implications for Practice

This article examines the relationship between delta-like protein 3 (DLL3) and achaete-scute homolog-1 (ASCL1) protein expression with the clinical features of 95 surgically resected small cell lung cancer (SCLC). DLL3 is attracting attention because rovalpituzumab tesirine (Rova-T), a DLL3-targeted antibody-drug conjugate, was developed recently. DLL3 and ASCL1 were highly expressed in patients with surgically resected SCLC. DLL3 and ASCL1 may be targets for the treatment of early-stage SCLC, including with Rova-T.
Does engineering of nanoshapes have antibacterial synergy with magnetic signal exposure?
Authors: El-Kaliuoby Mai Ismail , El-Khatib Ahmed Mohamed , Khalil Alaa Mahmoud
Published: May 2019

The widespread use of antibiotics in diverse areas led many bacterial strains to become highly resistant. Thus, considerable concerns were recently raised to overcome and/or to find other alternatives to the use of antibiotics. In this context, combining antibacterial effects of exposure to pulsed magnetic signals and supplying of silver nanoparticles recently indicated a significant bactericidal impact. The interplay between silver nanoshapes (spheres, rods and prisms) and exposure to pulsed magnetic signals (0·32 mT, 20 Hz–30 min) was studied to explore whether engineering of silver nanoshapes has a synergistic antibacterial effect against Klebsiella pneumoniae. In this regard, the bacterial growth rates, kinetics and enzymatic changes (protein leakage and lactate dehydrogenase levels) were investigated. The results confirmed that silver nanoprisms with a higher specific-area-to-volume ratio and appreciable analogy of charge distribution exhibit the highest synergistic inhibition effect with pulsed magnetic signals by 35% at 10 parts per million concentration. Moreover, the coupling of magnetic signals with nanoprisms showed significant cellular injuries and loss of membrane integrity as a consequence of the elevation of lactate dehydrogenase and protein leakage levels by more than fivefold and 15-fold, respectively. These new findings substantiate the antibacterial synergism of silver nanoparticles with pulsed magnetic signals in a shape-dependent manner.
Farklı uygulamaların ümitvar gypsophila genotipinin (GA8) kesme çiçek performansı üzerine etkileri
Authors: KAYA Ayşe Serpil , AYDİNŞAKİR Köksal , ERDAL Şekip , KAZAZ Soner
Published: May 2019

Türkiye gypsophila türleri bakımından zengin
bir floraya sahiptir. Ancak günümüze kadar geliştirilen ve süs bitkileri
sektörüne kazandırılan gypsophila çeşidi bulunmamaktadır. Bu nedenle gypsophila
yetiştiriciliğinde üretim materyalinde dışa bağımlılık devam etmektedir. Bu
araştırma, Türkiye florasında yayılış gösteren gypsophila türleri arasında klon
seleksiyonu yoluyla geliştirilen Gypsophila
arrostii türüne ait ümitvar (GA8) isimli genotipin kesme çiçek performansı
üzerine dikim sıklığı (30x40 cm, 40x50 cm, 60x60 cm), dikim zamanı (Ekim,
Aralık, Nisan) ve gün uzunluğunun (14, 15 ve 16 saat) etkilerinin belirlenmesi
amacıyla 2016-2017 yılları arasında Batı Akdeniz Tarımsal Araştırma
Enstitüsü’nde yürütülmüştür. Çalışmada; çiçeklenmeye kadar geçen süre, çiçek
sapı uzunluğu ve kalınlığı, dal ağırlığı, vazo ömrü ve çiçek verimi
parametreleri incelenmiştir. Elde edilen sonuçlar değerlendirildiğinde dikim
zamanı olarak ekim ayı, dikim aralığı olarak 30x40 cm ve 16 saatlik gün
uzunluğu uygulamasının incelenen özellikler bakımından diğer uygulamalara göre
daha iyi sonuçlar verdiği belirlenmiştir.
Bazı zeytin çeşitlerinin çöğür anacı olarak kullanılma potansiyellerinin belirlenmesi
Authors: GÜL Hükümran , İSFENDİYAROĞLU Murat
Published: May 2019

Türkiye’de zeytin fidanı üretiminde kullanılan tescil
edilmiş çöğür anaçları, Frantoio, Leccino, Arbequina, Girit Zeytini ve Uslu
zeytin çeşitlerinin tohumlarından elde edilmektedir. Bu çalışmada, Türkiye’nin
farklı bölgelerine ait zeytin çeşitlerden daha üstün nitelikli yeni çöğür anacı
adaylarının saptanması amaçlanmıştır. Bu amaçla zeytin çekirdeklerinin
çimlenmesini kolaylaştırıcı ön uygulamalar araştırılmıştır. Çalışmanın ikinci
aşamasında, elde edilen çöğürlerin üzerine Domat çeşidi aşılanarak, aşı
başarısı saptanmıştır. Kimyasal aşındırma uygulamaları
zeytin çekirdeklerinin çimlenmelerini, kontrole göre %10-33 arasında değişen oranlarda
artırmıştır. %3 NaOH uygulaması çimlenme oranlarını arttırırken, saf H₂SO₄ çimlenmeyi
azaltmıştır. Çeşit ve uygulamalara bağlı olarak çimlenme oranları %5.8-76.4
arasında değişmiştir. Bu çalışma ile tescil edilmiş anaçların yanı sıra, denenen
Çekişte, Edincik Su ve Yağ Çelebi gibi diğer bazı çeşitlerin de yeterli
oranlarda çimlendikleri saptanmıştır. Aşı başarısı
açısından, çekirdeklerinin çimlenme oranı yüksek bazı çeşitlerin, aşı tutma
oranları düşük olmuştur. En yüksek aşı tutma oranı (%70) Marantelli
çöğürlerinde gözlenirken, en düşük oran (%23.3) Yağ Çelebi’de saptanmıştır. En
uzun aşı sürgünü (43.4 cm) Girit Zeytini’nde ölçülmüştür. Özellikle Gemlik,
Sarı Ulak ve Sinop no 1’de düşük sürgün uzunluğu değerlerinin (25.1-27.4 cm)
saptanması nedeniyle, bunların üzerlerine aşılanan çeşitlerde belli oranda
bodurluk sağlayabileceği sonucuna varılmıştır.
DÜNYADA PLANLI EV DOĞUMLARINA GÜNCEL BAKIŞ; RİSKLER VE FAYDALARI
Authors: KUMRU Doç. Dr. Pınar , TOPUZOĞLU Prof. Dr. Ahmet
Published: May 2019

DÜNYADA PLANLI EV DOĞUMLARINA GÜNCEL
BAKIŞ; RİSKLER VE FAYDALARIÖZET:Gelişmiş ülkelerde planlı
evde doğum oranı %0,1-%20 arasında değişmektedir. Son 20 yıldır özellikle
gelişmiş ülkelerde evde doğum planlayan kadın sayısında artış dikkat
çekicidir.   Planlı ev doğumlarında
çoğu literatürde birbiri ile uyumlu şekilde maternal sonuçları olumlu olarak
bildirilmişse de yenidoğan sonuçları açısından daha değişkendir.Daha önceki
araştırmalarda, düşük riskli kadında planlı ev doğumlarında, oksitosin
indüksiyonu, sezaryen/operatif doğum, doğum sonrası kanama, perineal
laserasyon, farmakolajik analjezik ihtiyacı ve epizyotomi gibi müdahale ve
maternal morbiditeler azalmış olarak bildirilmiştir.  Düşük riskli kadında
intrapartum fetal ölümler, yenidoğan ölümleri, düşük Apgar skorları ve
yenidoğan yoğun bakım ünitesine kabul açısından hastane ve ev doğumları
arasında fark bulunmadığını bildiren araştırmalar yanında artmış olumsuz
yenidoğan sonuçları gösteren araştırmalarda mevcuttur. Planlı ev doğumları
özellikle nulliparlar gebeler için küçük de olsa artmış yenidoğan riskleri ile
ilişkili bulunmuşken multipar gebeler için daha güvenli olduğu bildirilmiştir.
Fakat makat prezentasyon, çoğul gebelik, ve geçirilmiş sezaryen öyküsü olan
kadınlarda evde doğum önerilmemektedir. Kadınların ev
doğumlarında çevrelerini daha iyi kontrol edebildikleri, kendi şartlarını
belirleyebildikleri, istemedikleri müdahalelerden kaçınabilecekleri ve doğum
sırasında karar almada etkin rol alabildikleri için memnuniyetlerinin arttığı  bildirilmiştir.   Evde doğumda bildirilen
diğer avantaj ise maliyet etkinliğidir.Evde doğum konusunda,
radomize kontrollü çalışmalar yapmak mümkün değildir ayrıca araştırma
tasarımları ile ilgili bir çok zorluk vardır. Planlı ev doğumlarının sonuçlarını gebelik sırasında
alınan annelik bakımı, gebelik ve doğum sırasında destek veren obstetrisyen ve
ebelerin eğitim düzeyi, hastaneye uzaklık ve transfer koşulları gibi bir çok
faktör etkilemektedir.  Uluslararası kabul görmüş
standartlara dayanan uygulama ve kılavuzların kullanımı evde doğum güvenliği
için önemlidir.Evde doğum, klavuzlar rehberliğinde,
eğitimli ebe/obstetrisyen eşliğinde veya
sağlık sistemine entegre transfer sistemlerinin varlığında, düşük riskli
kadınlar için özellikle yenidoğan sonuçları açısından risklerin çok az veya
hastane doğumlarına benzer olduğu tespit edilmiştir.  Bu derleme, planlı ev
doğumlarını maternal ve neonatal sonuçlar ile olan ilişkisinini güncel
araştırmalar ışığında  gözden geçirecek
ve planlı ev doğumlarının riskleri ve faydaları tartışacaktır.

Anahtar Kelimeler: Ev doğumu, hastane doğumu, Düşük riskli gebelik,
riskler, faydalar, maternal sonuçlar, yenidoğan sonuçları
The Artist Nomad
Authors: Buhler Clinton Jacob
Published: May 2019

When Ronald Reagan infamously declared the Soviet Union to be the “Evil Empire” in 1983, he was playing upon a fundamental axiom of Cold War politics: that the world can be neatly divided between the First World of Capitalism and the Second World of Communism.  Equating this divide with that of good and evil only served to strengthen the notion that the two sides were mutually exclusive.  Personal politics were an extension of this perspective.  One was either a Capitalist or a Communist—to reject one was equivalent to embracing the other.  This paper examines the art of dissident artists such as Alexander Kosolapov, Leonid Sokov, and Ilya Kabakov; artists from the Soviet Union who were exiled to the West during the Cold War.  It seeks to better understand why these artists’ rebellion against the Soviet system did not translate into an embrace of Western culture upon arrival in America.  The roots of their critical artistic approach are interpreted through the prism of ideological nomadism which reveals their art to be deeply ambivalent.  These artworks are analyzed as a disruption to the binary understanding of the Cold War and Post-Soviet eras by their embrace of a liminal position in the overlap between the capitalist and communist cultural milieus.
iGEM Calibration Protocol - Flow Cytometry Fluorescence v1
Authors: Beal Jacob , Telmer Cheryl , Tennant Richard , Rutten Paul
Published: May 2019

This protocol can be applied to any strain of cell that can be safely run through a flow cytometer. For clarity, we have written it assuming E. coli DH5-alpha; to apply the protocol to another cell type, substitute the other cell type for any place where the protocol says [E. coli DH5-alpha]. This protocol has been written for measurement of GFP, YFP, or other yellow/green fluorescent proteins into MEFL units. To apply it to fluorescent proteins of other colors: Replace BBa_J364001 with a construct for strong expression of the other protein. For blue proteins (e.g., mTagBFP), measure with 405nm excitation and 450nm/50nm emission filter. Units will be MEC30. For red/orange proteins (e.g., mCherry), measure with 561nm excitation and 610nm/20nm or 620nm/15nm emission filter. Units will be MEPTR. For far-red / near-infrared proteins (e.g., IRFP), measure with 635nm excitation and 780nm/60nm or 750nm long-pass (LP) emission filter. Units will be MEAPCY7. To apply the protocol to multiple colors, add a positive process control for each color and use one of the tools on the iGEM Measurement Resources page to compensate measurements for spectral overlap. This protocol can be combined with bead-based cell size calibration.
UC Davis - Receipt of Vendor and Courier Mice v1
Authors: Kent Lloyd K.C.
Published: May 2019

Summary: The purpose of this document is to describe the procedure for accepting commercial vendor and courier-delivered (“Imports”) shipments of mice to the 2nd Street facility of the UC Davis Mouse Biology Program. This procedure is to be followed by administrative personnel responsible for accepting commercial or courier-delivered shipments of mice and by Vivarium personnel responsible for unpacking and caging mice. Transportation of mice is stressful and potentially dangerous to their health, especially in warm summer months. Mice need to be unpacked, have a health inspection, and housed in appropriate caging in appropriate numbers as soon as possible after arrival. This insures they are acclimated and in the best physical health prior to their use by the Mouse Biology Program.
U Mass - STZ-induced type 1 diabetes model v1
Authors: Kim Jason
Published: May 2019

Summary: Streptozotocin can selectively destroy the pancreatic β-cells with rapid and irreversible necrosis and can be used to generate a chronic model of hyperglycemia and type 1 diabetes.
U Cinn - Cholesterol Concentration v1
Authors: Tso Patrick , Lee Dana
Published: May 2019

Summary: In vitro quantification of cholesterol in serum or plasma is determined using an Infinity Total Cholesterol Assay kit. This assay enzymatically hydrolyzes the cholesterol esters in the sample to cholesterol and free fatty acids. The free cholesterol is further oxidized and combined with HBA to allow it to be quantitatively measured in the serum or plasma.
Case - Heavy Water Assays by GC-mass spectrometry v1
Authors: Brunengraber Henri
Published: May 2019

Summary: Heavy water can be used as a tracer for estimating metabolic rates (in vivo, in vitro) such as total energy expenditure (TEE) and fractional synthesis rates (FSR; eg. protein, lipids, triglycerides). When using both ²H²O and H²18O (DLW), TEE is estimated from the total production of CO² as measured by the differences in decay rates of labeled the 18O and ²H in body water over time following a single bolus of DLW (1). ²H²O can be used to estimate fractional synthesis rates of metabolic reactions such as those associated with proteins, lipids, triglycerides, and cholesterol (2,4). References: 1. Gas chromatography-mass spectrometry assay of the (18) O enrichment of water as trimethyl phosphate. Brunengraber DZ, McCabe BJ, Katanik J, and Previs SF. Anal Biochem 306: 278– 282 (2002). 2. Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis. Fang D, West RH, Manson ME, Ruddy J, Jiang D, Previs SF, Sonawane ND, Burgess JD, Kelley TJ.Respir Res.; 11:61 (2010). 3. Triglyceride synthesis in epididymal adipose tissue: contribution of glucose and non-glucose carbon sources. Bederman IR, Foy S, Chandramouli V, Alexander JC, Previs SF. J Biol Chem.; 284(10):6101-8 (2009). 4. Novel application of the "doubly labeled" water method: measuring CO2 production and the tissue-specific dynamics of lipid and protein in vivo. Bederman IR, Dufner DA, Alexander JC, Previs SF. Am J Physiol Endocrinol Metab.; 290(5):E1048-56 (2006).
Case - Lipid Analysis Assay by GC-mass spectrometry v1
Authors: Brunengraber Henri
Published: May 2019

Summary A known quantity of tissue / plasma is hydrolyzed and extracted after adding known amounts of internal standards: eg. heptadecanoic acid and cholesterol-d7. Fatty acids / cholesterol are analyzed as their trimethylsilyl derivatives using gas chromatography-electron impact ionization mass spectrometry (GCMS) (note: this protocol outlines the processing for palmitate and cholesterol; other fatty acids and sterols can be assayed using this preparation, see refs 1,2). References: 1. Triglyceride synthesis in epididymal adipose tissue: contribution of glucose and non-glucose carbon sources. Bederman IR, Foy S, Chandramouli V, Alexander JC, Previs SF. J Biol Chem. 2009, 284(10):6101-8. 2. Influence of diet on the modeling of adipose tissue triglycerides during growth. Brunengraber DZ, McCabe BJ, Kasumov T, Alexander JC, Chandramouli V, Previs SF. Am J Physiol Endocrinol Metab. 2003, 285(4):E917-25.
Case - Plasma Insulin Measurement by ELISA v1
Authors: Brunengraber Henri
Published: May 2019

Summary: Mouse Insulin ELISA kit is used for the non radioactive quantification of insulin in mouse plasma.