bluuebunny/crossref_metadata_2025 huggingface dataset:
Pendampingan Penyusunan RPP (Rencana Pelaksanaan Pembelajaran) Kurikulum 2021 Bagi Guru Bahasa Indonesia di MTs Negeri Se-Kabupaten Cirebon
Authors: Nuryanto Tato , Uswati Tati Sri , Mulyaningsih Indrya , Khuzaemah Emah
Published: May 2022

Pengabdian kepada masyarakat ini bertujuan untuk meningkatkan kompetensi guru Bahasa Indonesia di MTs se-Kabupaten Cirebon. Kegiatan ini dilaksanakan pada 20 November 2021 dengan 30 peserta. Metode yang digunakan adalah penyuluhan dan pendampingan. Pada pertemuan awal, para guru diberi penjelasan dan tanya jawab terkait HOTS, TPACK, Pembelajaran Abad 21, dan Pelajar Pancasila. Setelah dirasa cukup, para peserta dibagi menjadi tiga kelompok, yakni kelompok kelas 7, 8, dan 9. Masing-masing kelompok berjumlah 10 guru. Setiap kelompok menyusun RPP sesuai yang diampu. Hasil pendampingan menunjukkan bahwa belum semua peserta mengetahui tentang HOTS, TPACK, Pembelajaran Abad 21, dan Pelajar Pancasila. Peserta tidak yakin sudah menggunaan teknologi informasi secara efektif dalam pembelajaran. Peserta belum maksimal dalam menyusun RPP. Hal ini terkait keterbatasan waktu. Bahkan sebagian besar peserta tidak membawa laptop sebagai sarana untuk menyusun RPP. Kegiatan ini dirasa belum maksimal. Oleh karena itu, para peserta meminta agar kegiatan seperti ini dilaksanakan secara rutin dan berkelanjutan sesuai kesepakatan yang tertuang dalam Nota Kesepahaman antara Jurusan Tadris Bahasa Indonesia FITK IAIN Syekh Nurjati Cirebon dengan MGMP Bahasa Indonesia MTs Kabupaten Cirebon. This community service aims to improve the competence of Indonesian language teachers in MTs throughout the Cirebon Regency. This activity was on 19 November 2021, with 30 participants. The method used is counseling and mentoring. At the initial meeting, the teachers were explained and asked questions related to HOTS, TPACK, 21st Century Learning, and Pancasila Students. After it was deemed sufficient, the participants were divided into the 7th, 8th, and 9th-grade groups. Each group consisted of 10 teachers. Each group prepares lesson plans according to what they are taught. The results of the mentoring show that not all participants know about HOTS, TPACK, 21st Century Learning, and Pancasila Students. Participants were not sure that they had used information technology effectively in learning. Participants have not been maximal in compiling the RPP. This is related to time constraints. Most of the participants did not bring a laptop to prepare lesson plans. This activity is not optimal. Therefore, the participants requested that actions like this be carried out regularly and continuously according to the agreement in the Memorandum of Understanding between Tadris Bahasa Indonesia Department FITK IAIN Syekh Nurjati Cirebon and the Indonesian Language MGMP MTs Cirebon Regency.
Pembekalan Pendidikan Kewirausahaan Berwawasan Gender bagi Mahasiswa Perintis Wirausaha di Banjarmasin Menuju Pemberdayaan Ekonomi Masyarakat
Authors: Nurhikmah Nurhikmah , Rusvitawati Devi , Anisa Nor , Nadila Nadila
Published: May 2022

Kesenjangan gender masih terjadi di berbagai bidang pembangunan, salah satunya di bidang ekonomi. Pemahaman gender saat ini banyak dipengaruhi oleh budaya lokal dan dibangun dari pemikiran yang tidak tepat dan mengakibatkan munculnya praktik diskriminatif dan kekerasan di masyarakat. Oleh karena itu, harus ada pemahaman yang benar dan tepat tentang kesetaraan dan keadilan gender. Pemahaman tersebut dapat terus dilembagakan di semua lapisan masyarakat, termasuk Perguruan Tinggi dalam melaksanakan Tri Dharma. Pengabdian kepada Masyarakat (PkM) ini bertujuan untuk meningkatkan peran Universitas dalam memfasilitasi bimbingan teknis dengan menggunakan Strategi Pengarusutamaan Gender. Selain itu juga memberikan pembekalan, pelatihan, dan pendampingan bagi Mahasiswa pionir wirausaha yang berperan sebagai agen informasi, edukasi, dan komunikasi melalui Strategi Pengarusutamaan Gender dalam pembangunan nasional, khususnya pemberdayaan ekonomi masyarakat. Pendekatan Participatory Rural Appraisal (PRA) digunakan dalam pengabdian ini. Peserta, 25 Mahasiswa dari enam perguruan tinggi di Banjarmasin, difasilitasi menerapkan PRA untuk merencanakan, melaksanakan, memantau, dan mengevaluasi Kegiatan Kewirausahaan Mahasiswa berbasis gender. Alhasil, penyusunan program Kegiatan Kewirausahaan Mahasiswa yang responsif gender di Banjarmasin berhasil menumbuhkan kesadaran kuat Mahasiswa bahwa PRA adalah alat untuk mewujudkan pemberdayaan ekonomi. The gender gap still exists in the economic field. The current gender understanding is heavily influenced by local culture and built from inappropriate thinking, resulting in discriminatory practices and violence. Therefore, there must be a right and proper understanding of gender equality and equality.  The understanding can continue to be institutionalized in society through all levels, including Universities’ role in implementing the Tri Dharma. This study aimed to increase the University’s role in facilitating technical guidance for entrepreneurial pioneer students through Gender Mainstreaming Strategy in national development, especially community economic empowerment. A Participatory Rural Appraisal (PRA) was used to facilitate 25 students from six universities in Banjarmasin in planning, implementing, monitoring, and evaluating the Student Entrepreneurship Activities based on gender-oriented entrepreneurship. As a result, the preparation of programs for gender-responsive Student Entrepreneurship Activities in Banjarmasin has succeeded in raising students' strong awareness that PRA is a tool for realizing economic empowerment.
Pelatihan Olahraga Permainan Srampangan untuk Meningkatkan Aktivitas Fisik dan Nilai Kerja Sama bagi Anak-anak
Authors: Eko Nopiyanto Yahya , Pujianto Dian
Published: May 2022

Tujuan dari pengabdian ini adalah untuk meningkatkan aktivitas fisik dan nilai kerja sama bagi anak-anak di kelurahan Mangunharjo. Metode yang digunakan adalah ceramah dan demonstrasi. Pengabdian ini dilaksanakan di RT/RW 02/01 Kelurahan Mangunharjo pada tanggal 7-8 Maret 2021. Peserta kegiatan ini terdiri dari 15 anak-anak berusia 5 hingga 14 tahun. Teknik pengumpulan data menggunakan lembar observasi, wawancara, dan tes. Berdasarkan hasil pengabdian diketahui bahwa peserta kegiatan mengalami peningkatan pemahaman akan pentingnya aktivitas fisik dan nilai kerja sama. Sehingga dapat disimpulkan bahwa kegiatan ini mampu meningkatkan aktivitas fisik dan nilai kerja sama bagi anak-anak.This service aims to increase physical activity and the value of cooperation for children in the Mangunharjo village. The method used is lecture and demonstration. This service was held at RT/RW 02/01 Mangunharjo Village on March 7-8, 2021. This activity consisted of 15 children aged 5 to 14 years—data collection techniques using observation sheets, interviews, and tests. Based on the service results, it was found that the activity participants experienced an increased understanding of the importance of physical activity and the value of cooperation. So, it can be concluded that this activity can increase physical activity and the value of cooperation for children.
Pencegahan Hipertensi dengan Penerapan Tidur Yang Berkualitas Pada Masyarakat Di RT 08 / Rw 08 Kelurahan Lenteng Agung Jakarta Selatan
Authors: Sihura Sancka Stella Ganiasnda , Gunardi Saiful , Pratiwi Panca , Martin Edo Fidelis , Suratinah Suratinah
Published: May 2022

Hipertensi merupakan penyebab utama yang mengakibatkan kematian jantung dan disabilitas, dan sekitar 1.3 miliar penduduk di seluruh dunia menderita penyakit tersebut. Hasil observasi di lapangan didapatkan bahwa masyarakat sering mengalami keluhan nyeri kepala, disertai dengan peningkatan tekanan darah yang cukup signifikan terutama pada masyarakat lanjut usia. Edukasi terkait peningkatan kualitas dan durasi tidur menjadi salah satu strategi tepat yang dapat dilakukan mengingat terdapat hubungan yang signifikan antara durasi tidur yang pendek dan hipertensi. Metode dilakukan dengan langkah-langkah melakukan peninjauan ke lingkungan RT 008/ RW 008 pada tanggal 27 Januari 2022, melakukan observasi dan pengukuran tekanan darah serta melakukan pre-test terkait tingkat pengetahuan masyarakat terhadap tidur yang berkualitas, melakukan edukasi terkait strategi tidur yang berkualitas dan merekamnya dalam video agar masyarakat bisa mengulang materi edukasi setiap hari jika diperlukan, melakukan post-test terkait tingkat pengetahuan masyarakat terhadap strategi tidur yang berkualitas, melakukan evaluasi, melakukan analisis data univariat dengan pengumpulan data yang disajikan dalam bentuk tabel distribusi frekuensi. Hasil post-test menunjukkan responden mengalami peningkatan pengetahuan terkait hipertensi dan sleep management sebesar 94,16%. Penyuluhan kepada masyarakat terkait strategi tidur yang berkualitas mempunyai manfaat untuk menurunkan resiko hipertensi. Hypertension is the leading cause of cardiac death and disability, and about 1.3 billion people worldwide suffer from the disease. The observations showed that people often experience headaches, accompanied by a significant increase in blood pressure, especially in the elderly. Education related to improving sleep quality and duration is one of the appropriate strategies that can be carried out, considering a significant relationship between short sleep duration and hypertension. The method is carried out by the steps of conducting a review of the citizen, observing and measuring blood pressure as well as conducting a pre-test related to the level of public knowledge about quality sleep, conducting education related to quality sleep strategies, and recording it in the video so that the public can repeating educational materials every day if needed, conducting post-tests related to the level of public knowledge of quality sleep strategies, conducting evaluations, conducting data analysis. The post-test results showed a significant increase (94.16%) in knowledge related to sleep quality before and after counselling. Counselling the community regarding quality sleep strategies has benefits for reducing the risk of hypertension.
Making Siberian Turkic Printed Collections Visible
Authors: Erdman M. J.
Published: May 2022

In the age of big data, small collections are often overlooked. Too numerically few to cause waves, the issues and problems that affect them are rarely addressed by cataloguing institutions. In the present paper, the author focuses on one such collection – holdings of Siberian Turkic materials at the British Library – to explore how homogenizing trends impact the cataloguing of these materials. The author concentrates particularly on aspects related to MARC21 requirements and authority files, and how these work to occlude the unique printing histories of Siberia’s indigenous Turkic languages. The author concludes that these trends may be overcome by treating the collections qualitatively through the creation of collection guides, addressing the inconsistencies in MARC21 and Library of Congress authority files, and outreach to and engagement with contemporary Siberian Turkic communities.
Formation of the identity of a modern university in the logic of the neo-institutional concept of D. Nort
Authors: Nikitenko E. V.
Published: May 2022

Introduction. The change in the general concept of university development in Russia in the context of the University 3.0 model allowed us to consider the university as an independent economic agent on the market. This allowed to apply neo-institutional approach of Douglass C. North.

Purpose setting. The process of shaping a new identity of higher education is determined by the ongoing institutional renewal. The article is devoted to identifying and analyzing the conditions that determine the formation of a new university identity in the logic of D. North’s neo-institutional concept.

Methodology of the study. The appeal to the neo-institutional approach arose in connection with the need to trace and assess the changes that have occurred and will occur to the university in the context of interaction with society, government and business, as well as to identify the set of conditions that will contribute to the formation of a new identity of the university. The concepts of formal and informal constraints, which determine the possible institutional changes, were considered as methodological concepts of D. North’s theory.

Results. An important condition for the functioning of an institution is a set of formal and informal attitudes, to which we respectively refer laws/codes, on the one hand, traditions and customs on the other. These concepts are closely related to the concepts of ideology and the market, which also influence changes in the system of restrictions. According to D. North, institutional changes are possible when formal and informal frameworks are updated. The article shows that with formal changes brought from above, the image of classical university as a social institution is still preserved, the market is also not ready to perceive the university as an independent economic agent, as one of the stakeholders of entrepreneurial and innovative activity.

Conclusions. The problem of contradiction between the preferences of subjects of educational, entrepreneurial and innovative activity becomes obvious. Serious restraining factors are the absence of regulatory framework explaining the mechanism of interaction between private business and the state, the preservation of the image of a classical university, the lack of coherence and consistency of institutional changes.
The impact of the general data protection regulation of the european union on the legal regime of international transfers of personal data
Authors: Ortega Giménez Alfonso
Published: May 2022

International data transfers involve a flow of personal data from Spanish territory to recipients established in countries outside the European Economic Area. It is the European Commission that decides, with effects for the whole Union, that a third country, a territory or a specific sector of a third country, or an international organisation offers an adequate level of data protection, thus providing legal certainty and uniformity throughout the Union with regard to the third country or international organisation.
Oxygen Uptake Response to Switching Stairs Exercise by Non-parametric Modeling
Authors: Yu Hairong , Xie Tian , Su Steven
Published: May 2022

Oxygen uptake plays a vital role in evaluating endurance performance during exercise and is widely used for metabolic assessment. In this study, the oxygen uptake during the exercise phase (i.e. ascending or descending) of the stairs exercise are recorded and the experimental dataset features ten participants and a range of different exercise periods. Based on the designed experiment protocol, a non-parametric modeling method with kernel-based regularization is generally applied to estimate the oxygen uptake changes during the switching stairs exercise that is close to the daily life status. Compared with the fixed-order models on accuracy, stability and compatibility, the modeling results demonstrate the effectiveness of the non-parametric modeling approach. The influence of exercise duration on estimated fitness reveals that the model of the phase-oxygen uptake system is not time-invariant related to respiratory metabolism regulation and muscle fatigue. Accordingly, it allows us to study the humans' conversion mechanism at different metabolic rates and facilitates the standardization and development of exercise prescriptions.
Integrated analysis of LRP2 mutation to immunotherapy efficacy in pan-cancer cohort
Authors: Li Chunbo , Ding Yan , Zhang Xuyin , Hua Keqin
Published: May 2022

Purpose Immunotherapy has emerged as a novel therapy, while many patients are refractory. Although, several biomarkers have been identified as predictive biomarkers for immunotherapy, such as tumor specific genes, PD-1/PD-L1, tumor mutation burn (TMB), and microsatellite instability (MSI), results remain unsatisfactory.Methods We investigated the characteristics of low-density lipoprotein receptor-related protein 2 (LRP2) mutation in the cancer genome atlas (TCGA) and explored the potential association of LRP2 mutation with immunotherapy. Characteristic of LRP2 mutation in 33 cancer types was analyzed using large-scale public data. The association of LRP2 mutation with immune cell infiltration and immunotherapy efficacy was evaluated. Finally, a LPR2 mutation signature (LMS) was developed and validated by TCGA-UCEC and pan-cancer cohorts. Furthermore, we demonstrated the predictive power of LMS score in independent immunotherapy cohorts by performing a meta-analysis.Results Our results revealed that patients with LRP2 mutant had higher TMB and MSI compared with patients without LRP2 mutation. Meanwhile, LRP2 mutation was associated with higher infiltration level of immune cells and higher expression of immune-related genes and signaling pathways. Patients with LRP2 mutation presented long overall survival (OS) after immunotherapy. In endothelial cancer (EC), patients with LRP2 mutation belonged to the POLE and MSI-H type of EC molecular types, with a better prognosis. Finally, we developed a LRP2 mutation signature (LMS), that was significantly associated with prognosis in patients receiving immunotherapy.Conclusion These results indicated that LRP2 mutation can serve as a biomarker for the personalization of cancer immunotherapy. Importantly, LMS is a potential predictor of patients prognosis after immunotherapy.
Identification of Health Effects of Complex Air Pollution in China
Authors: Zhao Yuxin , An Xingqin , Sun Zhaobin , Li Yi , Hou Qing
Published: May 2022

With the progress of air pollution control in China, the concentration of particulate matter has decreased, but the concentration of ozone has increased, the problem of complex air pollution has become more severe, posing a serious threat to public health. However, there is less study on the health effects of complex air pollution in China. Instead of introducing pollutant concentrations directly, we converted them into a set of predictors to prevent collinearity and other problems will occur when the concentrations of multiple correlated pollutants are introduced in general multi-pollution models. Based on different combinations of PM2.5, NO2, and O3 concentration levels, air pollutant constituent condition is divided into eight types, including three single-pollutant types and four multi-pollutant types. The health effects of different pollution types on mortality in eight typical Chinese cities from 2013 to 2016 were evaluated using a generalized additive model. The results from eight cities collectively indicate that multi-pollutant type leads to a higher impact on mortality risk than single-pollutant type. Type 7 with higher PM2.5, O3, and NO2 and type 4 with higher PM2.5 and NO2 have a greater relative risk among them. In most northern cities, the multi-pollutant type has a higher mortality effect in the warm season, but the single-pollutant type with high PM2.5 has a higher effect in the cold season. In southeastern cities, the multi-pollutant type had a higher mortality effect in both seasons. The results also showed that the excess risk of multi-pollutants was less than the simple sum of individual air pollutants effects, partially false conclusions would have been reached by ignoring the presence of interactions between air pollutants. The result further highlights the urgency and necessity of moving towards a multi-pollutant approach in air pollution health research under the background of atmospheric emission reduction and global warming.
Antimalarial potential of Gymnema inodorum leaf extract and dihydroartemisinin combination against Plasmodium berghei infected mice
Authors: Ounjaijean Sakaewan , Somsak Voravuth
Published: May 2022

Chemotherapy plays a crucial role in malaria control. However, the main obstacle to treatment has been the rise of parasite resistance to most antimalarial drugs. Artemisinin-based combination therapies (ACTs) remain today’s most effective antimalarial therapies. However, malaria parasite tolerance to ACTs is now increasingly prevalent, especially in Southeast Asia. Consequently, this creates the need for effective alternative antimalarials. Interestingly, the effectiveness of Gymnema inodorum leaf extract (GIE) as an antimalarial candidate was reported earlier. Therefore, this study sought to evaluate the antimalarial potential of dihydroartemisinin (DHA) based combination therapy with GIE against Plasmodium berghei in a mouse model. Drug assessment was carried out on P. berghei ANKA (PbANKA) using the standard 4-day test for determination of fifty percent effective dose (ED50) of DHA and GIE individually. Moreover, the combination of DHA and GIE was also evaluated based on fixed-ratio strategy, 100/0, 80/20, 60/40, 40/60, 20/80, and 0/100 of ED50 of DHA and GIE, respectively. Overall, 2 mg/kg and 100 mg/kg of DHA and GIE resulted in ED50 against PbANKA. In combination, the ratio of 60/40 (DHA/GIE) had a considerable the highest antimalarial activity significantly (p < 0.001) against PbANKA with 88.95% inhibition that indicated a synergism efficacy (CI value = 0.68695). Additionally, this ratio was protected PbANKA infected mice from BW loss and PCV reduction with prolonged survival time throughout the 30 day follow up. This study points to better efficacy of GIE as an alternative drug partner in combination to enhance antimalarial efficacy of DHA against PbANKA infected mice.
A Combination of UV curing and thermal Treatment successfully overcomes PDMS Curing Inhibition on 3D-Printed low-cost-resin Molds
Authors: Grigorov Emil , Kirov Boris , Galabov Vassil
Published: May 2022

We investigate the influence of thermal and UV post-curing treat- ments on 3D printed resin mold and the quality of a pored poly- mer, polydimethylsiloxane, utilized in the world of microfluidics.
Seroprevalence of Toxoplasma gondii and associated risk factors among pregnant women attending antenatal care in Ilala Municipality, Dar es Salaam, Tanzania
Authors: Lushina Mayala , Mushi Vivian , Tarimo Donath , Babafemi Emmanuel Oladipo
Published: May 2022

BackgroundToxoplasma gondii (T. gondii) infection during pregnancy is associated with a variety of complications to the mother and baby. In Tanzania, there is a paucity of data on exposure T. gondii infection among pregnant women and the associated risk factors. Therefore, this study investigated the seroprevalence of T. gondii and associated factors among pregnant women attending antenatal care in Ilala Municipality, Dar es Salaam.MethodsA cross-sectional study was carried out among 383 pregnant women attending antenatal health care. A five mL of blood sample was collected from each of the recruited pregnant women, processed to obtain serum, and tested for the presence of IgG and IgM anti-T. gondii specific antibodies. A structured questionnaire was used to gather information on the risk factors predisposing pregnant women to the infection. Data analysis was performed using descriptive statistics and logistic regression. ResultsOf the 383 participants, 104 (27.2%) were positive for antibodies specific to T. gondii; 102 (26.63%) were positive only for IgG, and 2 (0.52%) were positive both for IgM and IgG antibodies. Significantly risk factors for T. gondii infection included: increased maternal age (34-39) years [AOR = 3.714, 95% CI:1.522-9.062), eating unwashed fruits [AOR = 7.385, 95% CI:3.994-13.658,], not washing hand with soap after meat preparation [AOR = 7.525, 95% CI:3.403-16.642], consumption of undercooked meat [AOR = 3.746, 95% CI:1.945-7.217], and consumption of raw vegetable [AOR = 1.986, 95% CI:1.038-3.800]. ConclusionsThe seroprevalence of T. gondii infection (27.2%), indicates ongoing transmission hence the need for regular screening during antenatal care and establishment of a control program.
Role of Denosumab in the Management of Giant Cell Tumor, A cross sectional study
Authors: Saeed Javeria , Ahmed kaleem sohail , Shamsi Zaid , Umer Masood
Published: May 2022

Background Giant cell tumor (GCT) of bone is a benign lesion which is characterized by presence of multinucleated osteoclasts type giant cells. Nuclear factor kappa B (RANKL) serves as the trigger factor for osteoclasts cells formation. Although surgery is the primary treatment for GCT of bone but recurrence remains the concern. Therefore, the development of denosumab, a monoclonal antibody for treating GCT for both primary and recurrent disease cases. The present study aims to show the role of denosumab along with surgery when given as neo-adjuvant drug to patients of GCT for their treatment.Methodology:A total of 23 patients diagnosed with GCT were included in this study from January 2016- December 2019 and all of these patients had received neo adjuvant denosumab dose of 120 mg SC on day 0, 15, 30 & 45. All patients were treated at the section of Orthopedics, department of surgery, Aga Khan University hospital, Karachi. Other benign lesions were excluded from the study.Results Out of 23 patients we had 12 (52.2%) males and 11 (47.8%) females. The mean age of our patients was 34 13.8 years and mean follow up duration of all patients was 20.5±10.7 months. There were 15 (65.2%) primary cases of GCT while 8 (34.8%) were recurrent cases. In 8 (34.8%) of the cases primary site of lesion was distal femur followed by 7(30.4%). In surgical procedure 20 (87%) patients underwent wide margin excision and only 3 (13%) had intralesional curettage. Reconstruction was performed in 21 patients which consist of bone grafting in 9 patients and mega prosthesis insertion in 12 patients. Only two patients had no reconstruction. On final histopathology, there was no residual GCT and we observed no denosumab induced adverse effects. Post-operative complications included wound infection and peri prosthetic infection in 3 patients. On follow-up we had 4(17.4%) cases of recurrence that were offered revision surgery while 19 (82.6%) were disease free.Conclusion:Denosumab has shown successful results in treating patients with GCT along with surgical intervention and it can be a best option for treating recurrent disease as well.Research registration no: researchregistry4695
Vibrational Resonance and Control of the Response of a Nonlinear Tilted Cantilever Beam under Combined Multi-harmonic Low and High-Frequency Excitations
Authors: Sahoo Pradyumna Kumar , Chatterjee Shyamal
Published: May 2022

This paper investigates the vibrational resonance and control of the response of a nonlinear tilted cantilever beam under combined multi-harmonic low and high-frequency excitations. The basic system considers the first mode of vibration of a nonlinear tilted beam under parametric and direct base excitation with the frequency components locked in 2:1 ratio. Such a system is susceptible to parametric amplification of resonance, which may be detrimental for structural health in many mechanical systems. The primary objective of the research is to investigate the efficacy of high-frequency excitation in controlling parametric amplification in the system. To the best of the authors' knowledge such a study was not reported previously in the literature. The slow dynamic equations of the system are obtained using the method of direct partition of motion (MDPM), which is then analysed using the harmonic balance method (HBM). It has been shown that the high-frequency base excitation has a considerable influence on the effective damping and stiffness of the system under consideration. The results of the research demonstrate that high-frequency excitation can effectively control the parametric amplification by shifting the resonance curve in the frequency axis and eliminate undesirable bifurcations by increasing the effective damping. The phenomenon of vibrational resonance has been found for certain parameter values in the presence of high-frequency base excitation. Detail parametric studies are performed to reveal the rich nonlinear dynamics of the system, like multiple jumps in response, dual peaks, isolated resonance response (isola), loop formation in frequency response plot, etc. The numerical results reveal that high-frequency excitation transforms chaotic responses into regular periodic responses. Direct numerical simulations are performed in MATLAB SIMULINK to validate the analytical results.
Effect of nifedipine administration before embryo transfer on reproductive outcome in ICSI cycles, a double-blind control trial study
Authors: Basirat Fatemeh , Esmaeilzadeh Sedighe , Basirat zahra , Ghofrani Faeze , Golsorkhtabaramiri Masoumeh
Published: May 2022

Purpose: Nifedipine is a calcium channel blocker with smooth muscle relaxing properties. This study set out to investigate the efficacy of nifedipine administered orally before embryo transfer (ET) on the improvement of the Intracytoplasmic sperm injection (ICSI) outcome.This double-blind placebo-control trial study was carried out in Babol (Iran) in 2019- 2020. 200 infertile women undergoing ET treatments were randomly allocated to receive nifedipine 20 mg tablets placebo orally 30 minutes before ET. The primary outcome was the chemical pregnancy rate. Implantation rate and clinical pregnancy were considered as the secondary outcome. Result: There is no significant difference (P> 0.05) in the number of oocytes and quality of embryos in the nifedipine and placebo groups. Despite increases in the rate of a chemical pregnancy, there were no statistical differences in the study group versus the comparison group (24% vs 14%, P=0.1, rate ratio 0.88, 95% CI, 0.77 to 1.01) respectively. In implantation rate, no statistically significant difference between the two groups was evident (20% vs 10%, P=0.07, rate ratio 0.88, 95% CI, 0.79 to 1.00) respectively. Also, no significant increase in clinical pregnancy was found compared with the placebo (17% vs 8%, P=0.26, rate ratio 0.90, 0.81 to 1.00).Conclusion: Nifedipine administered orally 30 minutes before embryo transfer did not improve the chemical pregnancy rate, implantation rate, or clinical pregnancy rate in women undergoing ICSI.
Hypoxia-induced reprogramming of glucose-dependent metabolic pathways maintains the stemness of human bone marrow-derived endothelial progenitor cells
Authors: Lin Dongni , Yan Kaihao , Chen Lingyun , Chen Junxiong , Xu Jianing , Xie Zhijing , Li Zhujun , Lin Shuo , Li Jinghuan , Chen Zhenzhou
Published: May 2022

BackgroundThe benefits of hypoxia for maintaining the stemness of cultured human endothelial progenitor cells (EPCs) have previously been demonstrated but the mechanisms responsible remain unclear. There is growing evidence to suggest a role for cellular metabolism in the regulation of stem cell fate and self-renewal. This study aimed at exploring changes in glucose metabolism and roles in maintaining EPC stemness under hypoxia.MethodsExtracellular flux analysis, LC-MS/MS and 13C tracing HPLC-QE-MS were used to establish EPC metabolic status. Then inhibitors of glucose metabolism were used to assess the impact of the dependent pathways on cell stemness. The key enzymes of glycolysis, tricarboxylic acid cycle (TCA), pentose phosphate pathway (PPP) and mitochondrial respiration were inhibited, and the cell survival rate, clone formation rate, mRNA expression of stemness markers, Nanog, Oct4, Klf4 and Sox2, and adenosine triphosphate (ATP) level were compared.ResultsReprogramming of pathways concerned with glucose metabolism was found under hypoxic conditions, including increased rates of flux through glycolysis and the pentose phosphate pathway (PPP), together with decreased flux through the tricarboxylic acid (TCA) cycle and mitochondrial respiration. Use of inhibitors of key enzymes of glycolysis, PPP, TCA cycle and the mitochondrial electron transport chain (ETC) revealed that inhibition of glycolysis or the PPP impaired cell proliferation under both normoxic and hypoxic conditions. By contrast, inhibition of pyruvate oxidation, via targeting of the TCA cycle or ETC, increased cell stemness under normoxic conditions. Moreover, promotion of pyruvate oxidation reversed the effect of hypoxia in maintaining cell stemness. Although hypoxia decreased mitochondrial ATP level, total cellular ATP level was unchanged, indicating that energy production does not play a major role in hypoxic effects. ConclusionIn summary, hypoxia-induced reprogramming of pathways involved in glucose metabolism maintained EPC stemness. Artificial manipulation of cell metabolism may be a way to regulate EPC stemness, thereby facilitating in vitro cell expansion.
Deep learning algorithm for the detection and 3D segmentation of maxillofacial bone lesions in CBCT
Authors: Yeshua Talia , Ladyzhensky Shmuel , Abu-Nasser Amal , Abdalla-Aslan Ragda , Boharon Tami , Itzhak-Pur Avital , Alexander Asher , Chaurasia Akhilanand , Leichter Isaac , Nadler Chen
Published: May 2022

Objectives: This study aimed to develop an automated deep-learning algorithm for the detection and 3D segmentation of incidental jaw lesions in maxillofacial CBCT scans.Materials and Methods: The dataset included 82 CBCT scans with and without histologically confirmed benign bone lesions, obtained from three CBCT devices using different imaging protocols. The dataset consisted of axial CBCT images and was divided into training dataset (20,214 axial images), validation dataset (4,530 axial images) and testing dataset (6,795 axial images). A Mask-RCNN based deep-learning algorithm segmented the bone lesion in each axial image. The analysis of sequential slices improved the mask RCNN performance and assisted in classifying each CBCT case as containing bone lesions or not. Thereafter, the algorithm generated a 3D segmentation of the lesions. Results: The accuracy of the algorithm for classifying each CBCT case as either containing bone lesions or not, was 100%. The algorithm performance for detecting lesions in individual axial images showed high sensitivity (95.9%) and high precision (98.9%).Conclusions: Our deep learning algorithm can detect incidental bone lesions in CBCT scans with high accuracy, high sensitivity and high precision and is highly recommended for bone lesion detection and follow-up in CBCT imaging. Clinical relevance: The increasing number of CBCT scans performed worldwide dictates a clinical need for developing an automated tool, which will review, detect and volumetrically demonstrate incidental bone lesions in CBCT scans with high accuracy and low false-positive rate.
Integrated shimming enables independently parallel NMR detection
Authors: Cheng Yen-Tse , Jouda Mazin , Korvink Jan G.
Published: May 2022

Two major technical challenges facing parallel nuclear magnetic resonance (NMR) spectroscopy, at the onset, include the need to achieve exceptional B0 homogeneity, and good inter-detector radiofrequency signal decoupling, and have remained technical obstacles that limit high throughput compound screening via NMR. In this contribution, we consider a compact detector system, consisting of two NMR ’unit cell’ resonators that implement parallel B0 shimming with parallel radiofrequency detection, as a prototype NMR environment, pointing the way towards achieving accelerated NMR analysis. The utility of our approach is established by achieving local field correction within the bore of a 1.05 T permanent magnet MRI. Our forerunner platform suppresses signal cross coupling down to −30 dB under a geometrical decoupled scheme, leading to a halving of the necessary inter-coil distance. In this permanent magnet enviroment, two decoupled parallel NMR detector sites simultaneously achieve improved spectral linewidth, from 270 Hz to 24 Hz.
CNBP restricts SARS-CoV2 by regulating IFN and disrupting RNA-protein condensates
Authors: Fitzgerald Katherine , Chen Yongzhi , Lei Xuqiu , Jiang Zhaozhao , Humphries Fiachra , Mustone Nicholas , Ramos Irene , Mutetwa Tinaye , Fernandez-Sesma Ana
Published: May 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evades antiviral immunity through the expression of viral proteins that block detection, signaling, interferon (IFN) induction, and IFN-stimulated gene (ISG) expression. Weak induction of type I IFNs is associated with a hyperinflammatory response in patients that develop severe COVID-19. Here we uncover a role for cellular nucleic acid-binding protein (CNBP) in restricting SARS-CoV-2. Typically, CNBP resides in the cytosol and, in response to RNA sensing pathways, undergoes phosphorylation, nuclear translocation, and IFNβ enhancer DNA binding to turn on IFNβ gene transcription. In SARS-CoV-2-infected cells CNBP coordinates IFNβ gene transcription. In addition, CNBP binds SARS-CoV-2 viral RNA directly. CNBP competes with the nucleocapsid (N) protein and prevents viral RNA and nucleocapsid protein from undergoing liquid-liquid phase separation (LLPS) forming condensates critical for viral replication. Consequently, cells and animals lacking CNBP have higher viral loads and CNBP-deficient mice succumb rapidly to infection. Altogether, these findings identify CNBP as a key antiviral factor for SARS-CoV-2, functioning both as a regulator of antiviral IFN gene expression and a cell intrinsic restriction factor that disrupts LLPS to limit viral replication and spread.
Low skeletal muscle mass may contribute to the progression of non-alcoholic fatty liver disease in men
Authors: Pan Xiao-Yan , Liu Wen-Yue , Zhu Pei-Wu , Li Gang , Tang Liang- Jie , Gao Feng , Huang Ou-Yang , Yuan Hai-Yang , Targher Giovanni , Byrne Christopher D. , Wang Xiao-Dong , Zheng Ming-Hua
Published: May 2022

Background/Purpose of the study: Although low skeletal muscle mass is associated with non-alcoholic fatty liver disease (NAFLD), it is currently uncertain whether there are associations between weight-adjusted appendicular skeletal muscle (ASM%), severity of histological features of NAFLD, and the patatin-like phospholipase domain-containing 3 (PNPLA3) rs738409 polymorphism. Our aim was to test for a modifying influence of the PNPLA3 rs738409 variant on the associations between ASM% and severity of NAFLD histological features.Methods: We enrolled 401 Chinese men with biopsy-proven NAFLD. Using a bioelectrical-impedance body composition analyzer (BIA, Inbody 720, Japan Inc., Tokyo), we calculated the ASM% as the percentage of total appendicular skeletal muscle mass (ASM, kg)/total body mass (kg) ×100.Results: Compared to those with high ASM%, patients with low ASM% (≤30.6) had a greater severity of individual histological features of NAFLD. These patients also had a higher risk of severe steatosis and non-alcoholic steatohepatitis (NASH) (adjusted-odds ratio [OR] 2.34, 95%CI 1.39-3.93, and adjusted-OR 2.22, 95%CI 1.30-3.77) even after adjustment for age, body mass index, diabetes, and serum creatinine levels. Carriage of the G allele of PNPLA3 rs738409 plus low ASM% was associated with an increased risk of severe steatosis and presence of liver fibrosis (OR 3.02, 95%CI 1.46-6.26, P=0.003 and OR 2.18, 95%CI 1.03-4.60, P=0.041 respectively), and there was a non-significant but borderline increased risk of NASH (OR 2.00, 95%CI 0.98-4.06, P=0.056).Conclusions: Low ASM% in the presence of carriage of G allele of PNPLA3 rs738409 is associated with more severe NAFLD.
Microvascular changes and correlation with visual loss in pituitary adenoma
Authors: Chen Yaying , Li Xiaojiao , Song Xiangyuan , Cong Lin , Zhang Yuyan
Published: May 2022

Purpose To determine microvascular changes and the correlation between microvascular interception and optic nerve damage in pituitary adenoma (PA) patients.Methods The vessel density of optic disc and macular retinal vessels was measured, using optical coherence tomography angiography (OCTA). It was performed that correlation between microvascular interception and optic nerve damage, including visual field and structural measurements. Results Seventy four eyes from 40 pituitary adenoma patients and 36 eyes from 24 healthy voluntary controls were recruited prospectively. Optic disc vessel density from PA patients with visual field defect (perimetric PA) was reduced and macular vessel density at superficial retinal capillary plexus (SCP) level from both perimetric and preperimetric PA patients was increased, compared to controls. Optic retinal vessel density was inversely correlated with visual field mean deviation and positively correlated with retinal structural measurements. Conclusions The retinal microvascular changes in pituitary adenoma patients presented as significantly decreased optic vessel density in perimetric stage and significantly increased SCP macular vessel density in preperimetric stage. Optic retinal vessel density in PA patients was associated with advanced visual field damage and optic nerve structural impairments. OCTA seems to be a reliable method to detect retinal microvascular changes and access the severity of visual loss in chiasmal compression caused by pituitary adenoma.
The link between the consumption of dairy products with 10-year Framingham risk Score among women
Authors: Salehi Zahra , Namazi Nazli , Azadbakht Leila
Published: May 2022

Background
Given that in Iran women are more at risk of cardiovascular diseases (CVDs) than men, the aim of the present study was to investigate the consumption of dairy products in relation to Framingham risk Score (FRS) and cardiovascular risk factors in women.
Methods
371 women aged 18 to 50 years were recruited in this cross-sectional study. A validated and reliable food frequency questionnaire was used to measure diet. A 10-year odds of developing CVD between participants was predicted using FRS.
Results
Higher intake of total dairy, low-fat and high-fat dairy products was associated with higher FRS, but receiving each dairy, in particular, had nothing to do with FRS. Doogh consumption was directly associated with fasting blood sugar (FBS) (Odds ratio (OR): 14.11, 95% confidence interval (CI): 1.97, 101.03; P = 0.999). Also intake of high-fat dairy was directly correlated with serum levels of triglyceride (TAG) (OR: 4.47, 95% CI: 1.75, 11.43; P = 0.999). However, no correlation was observed between dairy products and other CVD risk factors.
Conclusions
Overall, consumption of doogh and high-fat dairy products was correlated with greater serum levels of FBS and TAG, respectively. Intake of total dairy, low-fat and high-fat dairy products was also associated with higher FRS. Future studies are needed to elucidate the link between dairy consumption and risk factors of CVD to characterize gender differences.
Programmable positive and negative chemotaxis of polymeric vesicles driven by degradation
Authors: Wang Danni , Luan Jiabin , Zhang Shaohua , White Paul , Wilson Daniela
Published: May 2022

Chemotaxis enables organisms and cells to move either toward or away from specific cues. Artificial protocells capable of integrating both positive and negative chemotaxis into one system are still very rare outside the biological realm and often require elaborately designed catalytic reactions with complex adaptation processes. Furthermore, the chemotactic mechanism of artificial systems is not yet fully investigated and understood especially at the molecular level. Here, we introduce degradation as a new propulsion mechanism for chemotactic polymeric vesicles without any external modification of the system. The vesicles can be programmed to exhibit either positive or negative chemotaxis by sensing the degrading catalyst as the attractant or the degradation products as the repellents. We systematically examined the possible mechanisms of the chemotaxis by a flow-chart toolbox approach and highlight the degradation-driven mechanism involved at the molecular level. The programmable chemotaxis driven by degradation represents a conceptually novel way to guide the design of artificial chemotactic protocells and opens a new route for biodegradable systems in fields such as the active delivery or artificial communication under out-of-equilibrium conditions.
Microwave-Assisted Digestion Using Diluted Nitric Acid for determination of nutrients elements in different waste fruit of agroindustry by inductively coupled plasma optical emission spectrometry (ICP OES)
Authors: Guedes Wesley Nascimento , Costa Vinicius Câmara , Santos Ingrid Alves , Tavares Iasnaia Maria de Carvalho , Pimetel Adriana Bispo , de Oliviera Julieta Rangel , Franco Marcelo
Published: May 2022

The current study describes an analytical procedure of microwave-assisted digestion using diluted nitric acid for determination nutrients elements in different waste fruit of agroindustry by inductively coupled plasma optical emission spectrometry (ICP OES). The optimization step was performed using the Doehlert matrix being that the studied variables were nitric acid concentration (2.0 mol L− 1) and hydrogen peroxide volume (3.0 mL). The accuracy of the proposed procedure was accessed by analysis of four certified reference material (CRM) of apple leaves (NIST 1515), spinach leaves (NIST 1570a), rice flour (NIST 1568a) and tomato leaves (NIST 1573a). The proposed procedure was successfully applied to the determination of Ca, Mg, K, Na, P, Fe, Zn, Mn and Cu in different waste fruit of agroindustry. Considering some critical parameters such as cost, green chemistry, and analyst safety, the proposed procedure has advantages compared to classical microwave-assisted digestion procedures based on concentrated acids.
Understanding Frequency Stability of Power System with High Penetration of Renewables
Authors: Lim SungHoon , Park Jung-Wook
Published: May 2022

The authors have requested that this preprint be removed from Research Square.
Initiation of Renin-Angiotensin System Inhibitors and the Incidence of First Complete Remission in Patients with Primary Nephrotic Syndrome: A Nationwide Cohort Study
Authors: Shimizu Sayaka , Niihata Kakuya , Nishiwaki Hiroki , Shibagaki Yugo , Yamamoto Ryohei , Nitta Kosaku , Tsukamoto Tatsuo , Uchida Shunya , Takeda Asami , Okada Hirokazu , Narita Ichiei , Isaka Yoshitaka , Kurita Noriaki
Published: May 2022

Background
Renin-angiotensin system inhibitors (RASis) are recommended as the first-line therapy to reduce proteinuria in primary nephrotic syndrome. It is unclear whether RASis can induce complete remission (CR) in patients on immunosuppressive therapy.
Methods
This retrospective cohort study included adults with primary nephrotic syndrome from causes other than minimal change disease who were enrolled in the Japanese Nephrotic Syndrome Cohort Study from January 2009 to December 2010, with a 5-year follow-up period, and were not on RASis at enrolment. The exposure was a new RASi prescription, and the outcome was the first CR, defined as a urinary protein level < 0.3 g/day. Marginal structural models and Poisson regression were used to account for time-varying covariates and to estimate the causal effects of RASis on CR.
Results
Of the 84 eligible patients (median age, 65 years; males, 57%), 51 (61%) had membranous nephropathy. At baseline (1 month after renal biopsy and/or starting immunosuppressive therapy), 87% and 33% of patients were prescribed corticosteroids and calcineurin inhibitors, respectively. RASis were prescribed to 66% of patients during the study period. Sixty-five patients experienced CR during a median follow-up of 9.0 months (incidence remission rate, 5.05/100 person-months). RASi use was associated with a higher (adjusted incidence rate ratio [aIRR]: 2.27, 95% confidence interval [CI]: 1.06–4.84), and a lower (aIRR: 0.17, 95% CI: 0.04–0.68) CR rate in patients with membranous nephropathy and other pathologies, respectively.
Conclusion
RASis are beneficial as an adjuvant therapy for inducing remission in patients with membranous nephropathy.