bluuebunny/crossref_metadata_2025 huggingface dataset:
Microstructure Evolution and Performance of AA2024 Alloy Through Wire-Arc Additive Manufacturing Under Different Heat Inputs
Authors: Yang Jin , Wang Aimin
Published: November 2024

Wire-arc additive manufacturing (WAAM) is widely applied in the aerospace, automotive, defense, and other industries. As such, the study of the formation and evolution mechanism of grains to achieve precise control over the performance of additive parts is important. In this study, AA2024 high-strength Al alloy wire was used to produce WAAM specimens under different heat inputs by varying different parameters. Subsequently, their microstructure, phases, and tensile properties were analyzed. The results indicated that the predominant crystal comprised cellular crystals, columnar crystals and dendritic crystals. At a heat input of 5500–6200 kJ m−1, the internal morphology of the specimen, characterized by the significant presence of fine cellular crystals, was predominantly favorable. As the heat input increased, α (Al) and θ (CuAl2) phases were increasingly identified within the specimens, the grid-like distribution of the θ phase along the crystal boundaries became clearer, and the θ′ phase precipitation decreased. Solution treatment and aging of the specimen produced using the optimal WAAM parameters resulted in an ultimate tensile strength (UTS) of 398 MPa, yield strength (YS) of 284 MPa, and elongation of 11.3%. The results should serve as a basis for selecting suitable WAAM process parameters.
The Concept of the Estimation of Phase Diagrams (An Optimised Set of Simplified Equations to Estimate Equilibrium Liquidus and Solidus Temperatures, Partition Ratios, and Liquidus Slopes for Quick Access to Equilibrium Data in Solidification Software) Part I: Binary Equilibrium Phase Diagrams
Authors: Kőrösy Gergely , Roósz András , Mende Tamás
Published: November 2024

This paper presents equations derived from thermodynamic equations for calculating the liquidus and solidus temperatures, the liquidus slope, and the partition ratio for solidification simulations. The constants of these equations can be easily determined from measurement data obtained by digitalisation from known diagrams or can be calculated using a CALPHAD-based software. ESTPHAD has a hierarchical system; the developed functions of the binary systems are used in the calculation of the functions of the ternary systems, the functions of the ternary systems in the calculation of the function of quaternary systems, and so on. The developed method is demonstrated by processing the liquidus and solidus of Si–Ge isomorphous and Al–Mg and Al–Si eutectic equilibrium phase diagrams. The use of this method for calculating the functions of ternary systems will be shown in Part II. The advantages of this method are that the equations are simple, can be determined very quickly, and can be built into the simulation software very easily. The most significant advantage is that the calculation time is shorter by some order of magnitude than that of a CALPHAD-type calculation.
The Role of Chemerin in Upper Gastrointestinal Cancer
Authors: Mylonakis Adam , Frountzas Maximos , Lidoriki Irene , Kozadinos Alexandros , Kalfoutzou Areti , Karanikki Eva , Tsikrikou Iliana , Kyriakidou Maria , Theodorou Dimitrios , Toutouzas Konstantinos G. , Schizas Dimitrios
Published: November 2024

Background/Objectives: Chemerin, which is a multifunctional cytokine and adipokine, has been implicated in inflammatory and metabolic processes and might play a role in upper gastrointestinal (GI) malignancies, particularly gastric and esophageal cancer. The aim of this review is to explore the role of chemerin in the pathophysiology of upper GI cancers, as well as its potential as a biomarker for early detection and as a therapeutic target. Methods: A comprehensive review of recent studies about chemerin’s biochemical properties and interaction with its receptors, as well as its effects on inflammatory responses, immune regulation, and metabolic processes, was conducted. The clinical implications of chemerin for gastric and esophageal cancer were analyzed, whereas the potential therapeutic strategies targeting chemerin were discussed. Results: Elevated chemerin levels are associated with poor prognosis in gastric cancer and promote invasiveness and metastasis in esophageal cancer. Chemerin receptor antagonists show promising results in inhibiting cancer cell migration, invasion, and progression. Conclusions: Chemerin could represent a valuable prognostic biomarker and therapeutic target for upper GI cancers. Future observational studies should validate its clinical applications and investigate the efficacy of chemerin inhibitors as potential therapeutic targets.
Highly Flexible and Compressible 3D Interconnected Graphene Foam for Sensitive Pressure Detection
Authors: Li Wentao , Zhou Jianxin , Sheng Wei , Jia Yuxi , Xu Wenjie , Zhang Tao
Published: November 2024

A flexible pressure sensor, capable of effectively detecting forces exerted on soft or deformable surfaces, has demonstrated broad application in diverse fields, including human motion tracking, health monitoring, electronic skin, and artificial intelligence systems. However, the design of convenient sensors with high sensitivity and excellent stability is still a great challenge. Herein, we present a multi-scale 3D graphene pressure sensor composed of two types of 3D graphene foam. The sensor exhibits a high sensitivity of 0.42 kPa−1 within the low-pressure range of 0–390 Pa and 0.012 kPa−1 within the higher-pressure range of 0.4 to 42 kPa, a rapid response time of 62 ms, and exceptional repeatability and stability exceeding 10,000 cycles. These characteristics empower the sensor to realize the sensation of a drop of water, the speed of airflow, and human movements.
Chemical Composition and Spectral Characteristics of Different Colored Spinel Varieties from Myanmar
Authors: Wang Mengwei , Wang Mingying , Qi Yihui , Xue Yuan , Shi Guanghai
Published: November 2024

With the growth of the Myanmar spinel market in recent years, spinels of colors other than red, including gray spinels, have gained increasing popularity. In this study, we performed conventional gemological, spectroscopic, and chemical analyses on the less commonly studied gray, red, pink, and purple spinels from Mogok in Myanmar to investigate their chemical composition and color mechanisms. The Raman and FTIR spectral analyses indicated that the samples contained oxides of Mg-Al end-members and that the spectral peak positions of different colors were essentially the same. According to the major, minor, and trace elements of samples determined via EPMA and LA-ICP-MS, the purple and gray samples had the most prominent Fe contents, the red spinels had the highest Cr contents, and the pink samples had high V+Cr contents, with a certain amount of Fe. The UV–visible spectra indicated that the absorption spectrum of the gray samples was predominantly influenced by the Fetot content, particularly Fe2+. The color rendering of the purple spinels was also intimately associated with Fe. The absorption spectrum of the gray spinels was weaker but more concentrated at 458 nm than that of the purple varieties. Cr3+ and V3+ in the red spinels produced broad bands near 400 nm and 540 nm, respectively, while light pink spinels exhibited Cr3+ and V3+ absorption spectra but featured an additional absorption band at 460 nm due to Fe. This study complements other research on the coloration mechanisms of multi-color spinels from Mogok, especially gray spinels.
Preconcentrating Ultrafine Ilmenite Tailings Using a Laboratory-Scale Reflux Classifier
Authors: Liu Zhenqiang , Su Zhenhua , Liu Bing , Wang Yuhua , Zhang Yuxin , Zhong Xuqun , Chen Kangkang , Hu Xiaoxing , Lu Dongfang
Published: November 2024

China is rich in reserves of titanium, but a large amount of titanium resources is lost in the ultrafine tailings, and it is challenging to treat the ilmenite contained in ultrafine ore. The reflux classifier (RC), a novel gravity concentration technology, has been applied in the preconcentration of ultrafine ilmenite in this study. During this process, the feasibility of using RC for preconcentration of ultrafine ilmenite was explored through theory and conditional experiments. After one-stage preconcentration using RC, the ultrafine ilmenite ore with a TiO2 grade of 8.77% can be concentrated into a product with a TiO2 grade of 20.3% and a recovery rate of 82.8%. The tailings grade is as low as 2.44%, and the yield reaches 62.6%. The separation efficiency achieves 50.0%. Experimental results demonstrate that utilizing RC for the preconcentration of ultrafine ilmenite can avoid the influence of weakly magnetic gangue and achieve better results compared to a magnetic separator. Therefore, RC offers a more effective and affordable method for preconcentrating ultrafine ilmenite ore.
The Nature of the Magma Source for Jurassic Mafic Rocks in the Yanbian Area, Eastern Jilin Province: Constraints on the Subduction Process of the Paleo-Pacific Ocean
Authors: Geng Rui , Fan Zhenhua , Wang Zhixin , Li Yixin , Ye Bingyang
Published: November 2024

Early Jurassic mafic–ultramafic igneous rocks are sporadically exposed in the eastern part of northeastern China. Understanding their petrogenesis and geological implications is essential for elucidating the evolution of magma in subduction zones and the evolutionary history of the Paleo-Pacific Ocean. This study presents a detailed petrological, zircon U-Pb geochronological, and whole-rock geochemical analysis of the Jurassic hornblende gabbro in the Yanbian area of northeastern China. The emplacement age of the hornblende gabbro is constrained to 175.3 ± 1.6 Ma. All samples exhibit low SiO2 content, metaluminous properties, and geochemical characteristics indicative of a subduction environment. By integrating trace elemental ratios, such as Th/Sm, Th/Ce, Ba/La, Rb/Nb, Hf/Sm, Ba/Nb, and εNd(t), our data propose that the hornblende gabbro originated from a mixture of depleted mantle and approximately 10%–20% continental sediment, and that the magmatism is generated by processes involving metasomatic addition to the source mantle wedge by ‘supercritical’ fluids. Contrasting with several recent hypotheses, the magma of the Early Jurassic gabbro originated from the mantle wedge, incorporating contributions from sediment melts and ‘supercritical’ fluids, and formed during the westward subduction of the Paleo-Pacific Ocean. The Yanbian area in Jilin Province was primarily influenced by this westward subduction during the Early Jurassic.
Facile Doping and Functionalization of Molybdic Acid into Nanobiochar to Enhance Mercury Ion Removal from Water Systems
Authors: Mahmoud Safe ELdeen M. E. , Abdel-Fattah Tarek M. , Mahmoud Mohamed E. , Díaz Eva
Published: November 2024

Functionalized nanomaterials with surface-active groups have garnered significant research interest due to their wide-ranging applications, particularly in water treatment for removing various contaminants. This study focuses on developing a novel, multi-functional nanobiosorbent by synthesizing nanosized biochar from artichoke leaves (NBAL) and molybdic acid (MA). The resulting nanobiosorbent, MA@NBAL, is produced through a microwave-irradiation process, offering a promising material for enhanced environmental remediation. The characteristics of assembled MA@NBAL were evaluated from SEM-EDX, XPS, TGA, FT-IR, and zeta potential detection. The size of particles ranged from 18.7 to 23.7 nm. At the same time, the EDX analysis denoted the existence of several major elements with related percentage values of carbon (52.9%), oxygen (27.6%), molybdenum (8.8%), and nitrogen (4.5%) in the assembled MA@NBAL nanobiosorbent. The effectiveness of MA@NBAL in removing Hg(II) ions was monitored via the batch study method. The optimized maximum removal capacity of Hg(II) ions onto MA@NBAL was established at pH 6.0, 30.0 min equilibrium time, and 20 mg of nanobiosorbent, providing 1444.25 mg/g with a 10.0 mmol/L concentration of Hg(II). Kinetic studies revealed that the adsorption process followed a pseudo-second-order model, with R2 values ranging from 0.993 to 0.999 for the two tested Hg(II) concentrations, indicating excellent alignment with the experimental data. This suggests that the chemisorption mechanism involves cation exchange and complex formation. Isotherm model evaluation further confirmed the adsorption mechanism, with the Freundlich model providing the best fit, yielding an R2 of 0.962. This result indicates that Hg(II) adsorption onto the surface of MA@NBAL nanobiosorbent occurs on a heterogeneous surface with multilayer formation characteristics. The results of the temperature factor and computation of the thermodynamic parameters referred to endothermic behavior via a nonspontaneous process. Finally, the valid applicability of MA@NBAL nanobiosorbent in the adsorptive recovery of 2.0 and 5.0 µg/mL Hg(II) from contaminated real aquatic matrices was explored in this study, providing 91.2–98.6% removal efficiency.
Extended One-Way Waveguide States of Large-Area Propagation in Gyromagnetic Photonic Crystals
Authors: Li Xiaobin , Yan Chao , Li Zhi-Yuan , Liang Wenyao
Published: November 2024

We propose extended one-way waveguide states of large-area propagation in a photonic crystal waveguide consisting of two honeycomb gyromagnetic photonic crystals with opposite external magnetic fields. When the width of the waveguide is small enough, the edge states along both boundaries of the waveguide couple with each other strongly and thus create the so-called extended one-way waveguide states. Of note, this structure supports both even and odd extended states, which can be excited under different excitation conditions. For the odd mode, electromagnetic waves have opposite phase distributions along the centerline of the waveguide on both sides, while for the even mode, they have in-phase distributions on both sides. In addition, the odd and the even modes both have the large-area propagating property. Moreover, we have carried out a microwave experiment to verify the simulation results. The measured transmission spectrum shows that the structure has strong non-reciprocity, and the measured electric field distributions of the even and odd modes prove that it supports excellent large-area transmission behaviors. These results provide feasible ideas for achieving topological high-throughput transmission.
Evaluation of the Dietary Habits of Polish and Greek University Students in the Context of the Health Benefits of Their Diets
Authors: Widłak Patrycja , Malara Marzena , Tomczyk Łukasz , Dania Aspasia , Panagiotakou Georgia , Papoulia Georgia
Published: November 2024

Background/Objectives: Eating habits and behaviors play a central role in maintaining health and overall well-being. In the case of young students, they affect not only appearance and general mood but also cognitive ability, concentration, and broader learning ability. For this reason, it is essential for young people to have well-balanced and wholesome diets. Methods: The study included 186 Polish and 136 Greek university students majoring in sports. The research tool was the validated survey questionnaire FFQ-6, and the data collected were additionally used to calculate the healthy diet index (pHDI-10). Results: Based on the analysis of the collected data, the dietary habits of the students surveyed were assessed, taking into account, among other things, the frequency of consumption of sweets, salty snacks, processed foods, and sweetened drinks, but also healthy products, including vegetables and fruit or vegetable and fruit juices. The data collected made it possible to determine an index of the health value of the participants’ diet. Conclusions: The analysis of the collected data led to the conclusion that, more often than their Greek peers, Polish university students tend to reach for sweets, salty snacks, or sweetened drinks. Greeks were more likely to choose fruit and vegetables and vegetable and fruit juices. However, among both Poles and Greeks, the index of the health-promoting value of the diet is low, indicating a high risk of diet-related diseases of lifestyle. Similar trends can be observed among students of almost all nationalities, which makes it reasonable to consider the implementation of appropriate educational programs on nutrition and healthy lifestyle principles in general.
Comparison of Analgesic and Anti-Inflammatory Effects of Kale Extract Versus Ibuprofen After Impacted Mandibular Third Molar Surgery: A Randomized, Double-Blind Split-Mouth Clinical Trial
Authors: Chatupos Vuttinun , Neelawatanasook Sansanee , Sangutai Tidanut , Khanutwong Atit , Srichairatanakool Pattaranee , Tipsuwan Wachiraporn , Kerdto Onsaya , Paradee Narisara , Koonyosying Pimpisid , Srichairatanakool Somdet
Published: November 2024

Background/Objective: We assessed the analgesic and anti-inflammatory effects of kale extract (500 mg anthocyanin equivalent) in patients after mandibular molar surgery. Methods: In our randomized clinical trial, postoperative subjects (n = 20) aged 18–25 years old took kale extract or ibuprofen (400 mg) capsules for 7 days, or vice versa, after surgical removal of each impacted tooth. Their pain intensity was then assessed using a visual analogue scale (VAS). Moreover, salivary α-amylase (AA) activity, matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta2 (TGF-β2) concentrations were measured. Levels of VAS and AA decreased 7 days after the first and second molar extractions in the two treatment groups. Results: The kale extract was more effective than ibuprofen. MMP-9 and TGF-β2 levels were reduced on days 4 and 7 following the two extractions in the kale group, whereas they were reduced on days 4 and 7 following the first extraction in the ibuprofen group. There was a positive correlation between MMP-9 and TGF-β2. Thus, the consumption of the kale extract exerted analgesic and anti-inflammatory effects during the postoperative period in patients who had undergone molar extractions. In conclusion, anthocyanin-abundant kale extract is preferable when administered in a postoperative course and could reduce the need for a prescription of ibuprofen.
Nurse Practitioner and General Practitioner Error Rates in a Large Digital Health Service: A Retrospective Cohort Analysis
Authors: Talay Louis , Vickers Matt , Lu Daisy
Published: November 2024

Background: Nurse practitioners have been prescribing medication within a narrow scope of practice throughout the world for several decades as a means of meeting rising demand for community health services. Prominent medical bodies have alleged that the Australian government’s decision to remove the need for general practitioner collaboration in the context of a nurse practitioner prescribing medication compromises patient safety. Objectives: This study aimed to determine whether nurse practitioner prescribing increases patient risk relative to general practitioner prescribing in a large digital health service. Methods: Investigators retrospectively analyzed prescription errors from all audited consults of the Eucalyptus Australia service over a 6 month period between 1 October 2023 and 31 March 2024. Results: Of the 8359 consults, errors were observed in 911 (14.22%) of NP and 417 (21.37%) of general practitioner consults and this difference was found to be statistically significant, X2 (1, N = 8359), =57.33, p ≤ 0.001. No statistically significant difference was observed in the incidence of high-risk or never events between nurse practitioners and general practitioners. Most high-risk and never events pertained to medical contraindications, insufficient side-effect counselling, and the insufficient assessment of a patient’s medical history. Conclusion: These findings suggest that nurse practitioners are capable of safely performing patient assessments and prescribing medications for a select range of conditions in digital health services.
Neuroprotective Potential of Glycyrrhizic Acid in Ischemic Stroke: Mechanisms and Therapeutic Prospects
Authors: Li Yanwen , Wu Juan , Du Fang , Tang Tao , Lim Jonathan Chee Woei , Karuppiah Thilakavathy , Liu Jiaxin , Sun Zhong
Published: November 2024

Background/Objectives: Ischemic stroke is a leading cause of disability and mortality worldwide, with current therapies limited in addressing its complex pathophysiological mechanisms, such as inflammation, oxidative stress, apoptosis, and impaired autophagy. Glycyrrhizic acid (GA), a bioactive compound from licorice (Glycyrrhiza glabra L.), has demonstrated neuroprotective properties in preclinical studies. This review consolidates current evidence on GA’s pharmacological mechanisms and assesses its potential as a therapeutic agent for ischemic stroke. Methods: This review examines findings from recent preclinical studies and reviews on GA’s neuroprotective effects, focusing on its modulation of inflammation, oxidative stress, apoptosis, and autophagy. Studies were identified from major scientific databases, including PubMed, Web of Science, and Embase, covering research from January 2000 to August 2024. Results: GA has demonstrated significant neuroprotective effects through the modulation of key pathways, including HMGB1/TLR4/NF-κB and Keap1/Nrf2, thereby reducing neuroinflammation, oxidative stress, and apoptosis. Additionally, GA promotes autophagy and modulates immune responses, suggesting it could serve as an adjunct therapy to enhance the efficacy and safety of existing treatments, such as thrombolysis. Conclusions: Current findings underscore GA’s potential as a multi-targeted neuroprotective agent in ischemic stroke, highlighting its anti-inflammatory, antioxidant, and anti-apoptotic properties. However, while preclinical data are promising, further clinical trials are necessary to validate GA’s therapeutic potential in humans. This review provides a comprehensive overview of GA’s mechanisms of action, proposing directions for future research to explore its role in ischemic stroke management.
Antibacterial Effects of Essential Oils on P. aeruginosa, Methicillin-Resistant S. aureus, and Staphylococcus spp. Isolated from Dog Wounds
Authors: Sezener Kabay Merve Gizem , Inal Sinem , Gökmen Sedat , Ergüden Volkan Enes , Fındık Arzu , Güvenç Tolga , Kayhan Hülya , Güvenç Dilek
Published: November 2024

Background: Essential oils exhibit several biological activities such as antimicrobial, antioxidant, proliferative, and anti-inflammatory. This study was aimed at investigating the antimicrobial effects and cytotoxic activities of niaouli, palmarosa, and clove essential oils. Methods: Content analyses of these essential oils were carried out by gas chromatography–mass spectrometry. The antibacterial activity was screened against methicillin-resistant S. aureus ATCC 43300, P. aeruginosa ATCC 27853, P. aeruginosa PAO1, S. aureus ATCC 25923, and 44 isolates (22 P. aeruginosa isolates, 4 S. aureus isolates, and 18 Staphylococcus spp. isolates) obtained from dogs with previous wound infections who were included in the current study. The antimicrobial effects of essential oils were investigated using disk diffusion and minimum inhibition/bactericidal concentration methods. Additionally, the antibiofilm, protease, elastase, and gelatinase activities of the essential oils were evaluated. Different concentrations of each essential oil ranging from 10 to 1000 µg/mL were also analyzed in terms of cell viability by WST-8 assay in primary canine fibroblast cells. Results: The fibroblast cell viabilities of palmarosa, niaouli, and clove oils at a 1000 µg/mL concentration were 75.4%, 96.39%, and 75.34%, respectively. All the EOs were found to have bactericidal effects with MBCs/MICs of 0.015 to 0.5 µL/mL against P. aeruginosa, Staphylococcus isolates (p < 0.001). Palmarosa was found to have the largest inhibition zone diameter (20.5 ± 6.6, 16.4 ± 2.3) compared to other essential oils in the disk diffusion test against Staphylococcus spp. and P. aeruginosa (p < 0.001). But none of the EOs reduced protease, elastase, and gelatinase activities, which are some of the virulence properties of the tested bacteria. Conclusions: These results showed that palmarosa, niaouli, and clove essential oils act as potential antibacterial agents for dogs against P. aeruginosa, methicillin-resistant S. aureus, and Staphylococcus spp., without damaging the skin.
From Microcirculation to Aging-Related Diseases: A Focus on Endothelial SIRT1
Authors: Law Martin , Wang Pei-Chun , Zhou Zhong-Yan , Wang Yu
Published: November 2024

Silent information regulator sirtuin 1 (SIRT1) is an NAD+-dependent deacetylase with potent anti-arterial aging activities. Its protective function in aging-related diseases has been extensively studied. In the microcirculation, SIRT1 plays a crucial role in preventing microcirculatory endothelial senescence by suppressing inflammation and oxidative stress while promoting mitochondrial function and optimizing autophagy. It suppresses hypoxia-inducible factor-1α (HIF-1α)-mediated pathological angiogenesis while promoting healthy, physiological capillarization. As a result, SIRT1 protects against microvascular dysfunction, such as diabetic microangiopathy, while enhancing exercise-induced skeletal muscle capillarization and energy metabolism. In the brain, SIRT1 upregulates tight junction proteins and strengthens their interactions, thus maintaining the integrity of the blood−brain barrier. The present review summarizes recent findings on the regulation of microvascular function by SIRT1, the underlying mechanisms, and various approaches to modulate SIRT1 activity in microcirculation. The importance of SIRT1 as a molecular target in aging-related diseases, such as diabetic retinopathy and stroke, is underscored, along with the need for more clinical evidence to support SIRT1 modulation in the microcirculation.
Modified PEG-Lipids Enhance the Nasal Mucosal Immune Capacity of Lipid Nanoparticle mRNA Vaccines
Authors: Li Meng , Yi Jing , Lu Yicheng , Liu Ting , Xing Haonan , Wang Xiwei , Zhang Hui , Liu Nan , Wang Zengming , Zheng Aiping
Published: November 2024

Background/Objectives: Omicron, the predominant variant of SARS-CoV-2, exhibits strong immune-evasive properties, leading to the reduced efficacy of existing vaccines. Consequently, the development of versatile vaccines is imperative. Intranasal mRNA vaccines offer convenient administration and have the potential to enhance mucosal immunity. However, delivering vaccines via the nasal mucosa requires overcoming complex physiological barriers. The aim of this study is to modify PEGylated lipids to enhance the mucosal immune efficacy of the vaccine. Methods: The PEGylated lipid component of lipid nanoparticle (LNP) delivery vectors was modified with chitosan or mannose to generate novel LNPs that enhance vaccine adhesion or targeting on mucosal surfaces. The impact of the mRNA encoding the receptor-binding domain of Omicron BA.4/BA.5 on the immune response was examined. Results: Compared to the unmodified LNP group, the IgG and IgA titers in the chitosan or mannose-modified LNP groups showed an increasing trend. The chitosan-modified group showed better effects. Notably, the PEGylated lipid with 1.5 mol% of chitosan modification produced high levels of IgG1 and IgG2a antibodies, promoting Th1/Th2 responses while also generating high levels of IgA, which can induce stronger cellular immunity, humoral immunity, and mucosal immunity. Conclusions: The 1.5 mol% of chitosan-modified LNPs (mRNA-LNP-1.5CS) can serve as a safe and effective carrier for intranasal mRNA vaccines, offering a promising strategy for combating the Omicron variant.
Genome-Wide Identification of PR10 Family Members in Durum Wheat: Expression Profile and In Vitro Analyses of TdPR10.1 in Response to Various Stress Conditions
Authors: Khanfir Emna , Zribi Ikram , Dhouib Hanen , Ghorbel Mouna , Hamdi Karama , Jrad Olfa , Yacoubi Inès , Brini Faiçal
Published: November 2024

The functional characterization of PR10 proteins has been extensively studied in many plant species. However, little is known about the role of TdPR10 in the response of durum wheat (Triticum durum Desf.) to stress. In this study, we identified members of the T. durum PR10 family, which are divided into three major subfamilies based on phylogenetic analyses. The analysis revealed that tandem duplication was the primary driver of the expansion of the T. durum PR10 gene family. Additionally, gene structure and motif analyses showed that PR10 family genes were relatively conserved during evolution. We also identified several cis-regulatory elements in the TdPR10 promoter regions related not only to abiotic and biotic stress but also to phytohormonal responses. In response to abiotic stresses and phytohormones, several TdPR10 genes were highly expressed in the leaves and roots of durum wheat. Moreover, TdPR10.1 family members improve RNase activity, increase LDH protective activity under abiotic stress conditions, and ensure resistance to fungi in vitro. Collectively, these findings provide a basis for further functional studies of TdPR10 genes, which could be leveraged to enhance stress tolerance in durum wheat.
Screening for Drought Tolerance Within a Common Bean (Phaseolus vulgaris L.) Landrace Accessions Core Collection from the Lazio Region of Italy
Authors: Alicandri Enrica , Badiani Ester , Paolacci Anna Rita , Lo Presti Emilio , Caridi Roberta , Rea Roberto , Pati Francesco , Badiani Maurizio , Ciaffi Mario , Sorgonà Agostino
Published: November 2024

In the present work, a subset extracted from a core collection of common beans (Phaseolus vulgaris L.) landrace accessions from the Lazio region in central Italy was used to identify the most suitable drought-tolerant or -susceptible genotypes. By applying several morpho-physiological and agronomic selection criteria recommended by the available literature, we conducted a pre-screening experiment under controlled conditions on a primary group of 24 landraces. These landraces were chosen to represent the diversity in the Lazio region in terms of geographical provenance, elevation, landform, growth habit, customary water management in the field, and native gene pool. Pre-screening under controlled conditions allowed us to identify two subsets of landraces: one exhibiting the most drought-tolerance and one showing the most susceptibility to drought. These two subsets were then tested in field trials using two water treatments, i.e., normal irrigation versus no irrigation. Such field experiments were simultaneously conducted at two sites within the Lazio region, deliberately chosen to maximize their differences in terms of pedo-climatic conditions. This notwithstanding, the core findings from the two separate field experiments were remarkably consistent and coherent among each other, highlighting a notable degree of variability within the group of the tested landraces. In general, the morpho-physiological traits considered were found to be less responsive to water shortage than yield parameters. A statistically significant Landrace × Treatment interaction was found for almost all the yield parameters considered, suggesting that certain genotypes are more susceptible than others to water shortage. By taking into account the concept of “yield stability”, i.e., the ability to maintain comparatively high yields even under conditions of water scarcity, certain common bean landraces were found to be the most promising, in terms of drought tolerance. Two genotype pairs, in particular, could be of interest for studying the morpho-physiological and molecular mechanisms underlying drought tolerance in common bean, as well as for identifying quantitative trait loci associated with water scarcity, which could be beneficially employed in breeding programs. The results reported here also suggest that pre-screening under laboratory conditions, followed by targeted field trials, can constitute a reliable, efficient, and resourceful combined approach, in which morpho-physiological traits measured on juvenile plants might play a role in predicting drought tolerance at the agronomic level.
Uptake, Translocation, Toxicity, and Impact of Nanoparticles on Plant Physiological Processes
Authors: Djanaguiraman Maduraimuthu , Anbazhagan Veerappan , Dhankher Om Parkash , Prasad P. V. Vara
Published: November 2024

The application of nanotechnology in agriculture has increased rapidly. However, the fate and effects of various nanoparticles on the soil, plants, and humans are not fully understood. Reports indicate that nanoparticles exhibit positive and negative impacts on biota due to their size, surface property, concentration within the system, and species or cell type under test. In plants, nanoparticles are translocated either by apoplast or symplast pathway or both. Also, it is not clear whether the nanoparticles entering the plant system remain as nanoparticles or are biotransformed into ionic forms or other organic compounds. Controversial results on the toxicity effects of nanomaterials on the plant system are available. In general, the nanomaterial toxicity was exerted by producing reactive oxygen species, leading to damage or denaturation of various biomolecules. The intensity of cyto- and geno-toxicity depends on the physical and chemical properties of nanoparticles. Based on the literature survey, it is observed that the effects of nanoparticles on the growth, photosynthesis, and primary and secondary metabolism of plants are both positive and negative; the response of these processes to the nanoparticle was associated with the type of nanoparticle, the concentration within the tissue, crop species, and stage of growth. Future studies should focus on addressing the key knowledge gaps in understanding the responses of plants to nanoparticles at all levels through global transcriptome, proteome, and metabolome assays and evaluating nanoparticles under field conditions at realistic exposure concentrations to determine the level of entry of nanoparticles into the food chain and assess the impact of nanoparticles on the ecosystem.
Microencapsulated Microbial Seed Coating Could Improve Soil Environment and Maize Grain Yield in Saline Soil
Authors: Huo Qiuyan , Gong Min , Jiang Yawen , Yang Xi , Kong Meng , He Jiuxing , Zhang Qiang , Song Jiqing , Li Xinzhu , Han Wei , Mei Xurong , Lv Guohua
Published: November 2024

Soil salinization is one of the major challenges for modern agriculture, posing a great threat to soil health and food security. Field experiments were conducted to evaluate the effect of seed coating on soil environment and maize growth in saline soils. Three treatments were applied to maize seeds: coating with a microencapsulated microbial agent (ME), coating with microbial only (MB), and no coating (CK). High-throughput sequencing of soil bacterial and fungal 16S and ITS rRNA genes was performed using the Illumina HiSeq platform to analyze the effects of these treatments on soil bacterial and fungal diversity and community structure. Additionally, the influence of different treatments on endogenous hormones and yield of maize were investigated. It was found that the coating with a microencapsulated microbial agent led to decreases in pH and electrical conductivity (EC), while increasing the content of soil available phosphorus. This coating improved soil microbial diversity, significantly increasing the relative abundance of the main bacteria genera, Bacillus (34.9%), and the main fungal genera, Mortierella (190.4%). The treatment also significantly enhanced indole-3-acetic acid (IAA) by 51.2%, contributing to improvements in resistance to salt stress. The germination rate increased by 22.9%, the 100-grain weight increased by 12.7%, and grain yield increased by 14.3%. The use of the microencapsulated microbial agent effectively mitigated the adverse effects of salt stress on maize plants. This approach is beneficial for promoting sustainable agriculture in saline soils.
Investigation into the Internal Factors for the Catalytic Oxidation of Cyclohexane by Zr(IV)-Based Metal-Organic Frameworks
Authors: Wang Kechao , Tao Zhi-Peng , Chu Jia-Qi , Wang Shi-Ming , Han Zheng-Bo
Published: November 2024

Three porous Zr(IV)-based MOFs, UiO-66, MOF-808 and MOF-802, were selected to catalyze cyclohexane oxidation in order to reveal the intrinsic factors of the active site and catalytic performance. It was found that reducing the number of Zr6O8 ligand linkages could improve the catalytic efficiency of cyclohexane oxidation. The main reason for this is the different enrichment abilities of MOFs with different linkage numbers for cyclohexane, which was confirmed by the TPD of cyclohexane and also by GCMC simulations. Meanwhile, the catalytic effect of MOF-802 was lower than expected due to its low porosity and narrow inner pore size. The by-products were identified in detail by GC-MS, providing evidence for this catalytic mechanism. In addition, the potential of this catalyst for industrial applications in cyclohexane oxidation was demonstrated by optimizing the catalytic conditions.
Mechanical Behaviour of Green Epoxy Composites Reinforced with Sheep and Dog Wool from Serra Da Estrela
Authors: Antunes Cláudia , Costa Ana Paula , Vieira André Costa , Vieira Joana Costa
Published: November 2024

Environmental awareness has led industries and consumers to replace products derived from oil resources with products derived from natural sources. In the case of the composite materials industry, the replacement of synthetic fibres with natural fibres has increased in recent years. To study the influence that different types of natural fibres and different textile manufacturing techniques have on the mechanical properties of composites, bio-based epoxy matrix composites reinforced with different natural animal fibres were produced, some reinforced with sheep’s wool and others with dog wool, which were later subjected to bending and tensile tests. From the authors’ knowledge, there are few studies of composites produced with animal fibres, and even fewer with dog hair. The textile structures used as reinforcement were created using crochet, knitting, and weaving techniques. Prior to the composites production, the fibres were characterized by X-ray Diffraction (X-RD), and the yarns produced from these fibres were subjected to tensile tests. The results obtained suggest that the number of yarns and the diameter of the needles used during the production of the reinforcement have a significant impact on the mechanical properties of the composites. The green epoxy resin composites reinforced with sheep’s wool exhibit higher values of flexural strength, tensile strength, and Young’s modulus than those reinforced with dog wool, with average increases of 36.97%, 45.16%, and 72.99%, respectively. It was also possible to verify that the composites reinforced with woven fabrics and crocheted fabrics exhibit the highest values of tensile strength, flexural strength, and Young’s modulus. Additionally, the composites reinforced with woven fabrics exhibit the highest values of deformation at first failure/break and toughness.
One-Step Preparation of Both Micron and Nanoparticles
Authors: Guo Zihao , Zhang Zhiyuan , Cao Yunchen , Chen Chunyi , Wang Juan , Yang Haoran , Song Wenbin , Peng Yiyang , Hu Xiaowei
Published: November 2024

The complex materials comprised of both micron and nanometer-sized particles (MNPs) present special properties different from conventional single-size particles due to their special size effect. In this study, the MNPs could be simultaneously synthesized in a one-pot medium by soap-free emulsion polymerization, without harsh preparation conditions and material waste. In the whole process, the amphipathic siloxane oligomers would migrate to the mixed monomer droplet surface to reduce the surface energy of the system and further complete hydrolysis–condensation to obtain the SiO2 shell at the water–oil interface. On the one hand, the mixed monomers inside the above shell would migrate outward driven by the capillary force generated at the shell mesopore and be further initiated by the water-soluble initiator potassium persulfate (KPS), resulting in the formation of bowl-shaped micron particles with “lunar surface” structure. On the other hand, the residual mixed monomers dissolve in water and could be polymerized by initiating free radicals in the water phase to obtain popcorn-like nano-sized particles. The above two particles are clearly displayed in the SEM photos, and the DLS characterization further shows that the sizes of two particles are concentrated at 1.4 μm and 130 nm, respectively. Interestingly, the uniformity of obtained particles has a great relationship with the added amount of BA, and the perfect MNPs would appear when the St/BA feed mass ratio is 1:2. Moreover, the MNPs exhibit film-forming property, and the SiO2 component is evenly distributed in the formed coating. Thus, this study is not only beneficial to the theoretical research of soap-free emulsion polymerization but also to the application of multifunctional coatings.
Robust and Adaptive Control of a Soft Continuum Manipulator for Minimally Invasive Surgery
Authors: Wang Liujia , Chen Kaiwen , Franco Enrico
Published: November 2024

This article investigates the model-based control in configurations space of a soft continuum manipulator for minimally invasive surgery. The main control challenges for these types of systems are the presence of model uncertainties and nonlinearities. To this end, a sliding-mode controller, a Lyapunov redesign controller, and an adaptive controller have been designed and compared by means of simulations and experiments on a prototype. The results indicate that the adaptive controller yields better accuracy but a slower transient. Conversely, the sliding-mode controller and Lyapunov redesign yield a faster response but can result in chattering or steady-state errors.
Full-Waveform Inversion of Two-Parameter Ground-Penetrating Radar Based on Quadratic Wasserstein Distance
Authors: Lu Kai , Wang Yibo , Han Heting , Zhong Shichao , Zheng Yikang
Published: November 2024

Full-waveform inversion (FWI) is one of the most promising techniques in current ground-penetrating radar (GPR) inversion methods. The least-squares method is usually used, minimizing the mismatch between the observed signal and the simulated signal. However, the cycle-skipping problem has become an urgent focus of this method because of the nonlinearity of the inversion problem. To mitigate the issue of local minima, the optimal transport problem has been introduced into full-waveform inversion in this study. The Wasserstein distance derived from the optimal transport problem is defined as the mismatch function in the FWI objective function, replacing the L2 norm. In this study, the Wasserstein distance is computed by using entropy regularization and the Sinkhorn algorithm to reduce computational complexity and improve efficiency. Additionally, this study presents the objective function for dual-parameter full-waveform inversion of ground-penetrating radar, with the Wasserstein distance as the mismatch function. By normalizing with the Softplus function, the electromagnetic wave signals are adjusted to meet the non-negativity and mass conservation assumptions of the Wasserstein distance, and the convexity of the method has been proven. A multi-scale frequency-domain Wasserstein distance full-waveform inversion method based on the Softplus normalization approach is proposed, enabling the simultaneous inversion of relative permittivity and conductivity from ground-penetrating radar data. Numerical simulation cases demonstrate that this method has low initial model dependency and low noise sensitivity, allowing for high-precision inversion of relative permittivity and conductivity. The inversion results show that it, in particular, significantly improves the accuracy of conductivity inversion.
Removing Instrumental Noise in Distributed Acoustic Sensing Data: A Comparison Between Two Deep Learning Approaches
Authors: Gu Xihao , Collet Olivia , Tertyshnikov Konstantin , Pevzner Roman
Published: November 2024

Over the last decade, distributed acoustic sensing (DAS) has received growing attention in the field of seismic acquisition and monitoring due to its potential high spatial sampling rate, low maintenance cost and high resistance to temperature and pressure. Despite its undeniable advantages, DAS faces some challenges, including a low signal-to-noise ratio, which partly results from the instrument-specific noise generated by DAS interrogators. We present a comparison between two deep learning approaches to address DAS hardware noise and enhance the quality of DAS data. These approaches have the advantage of including real instrumental noise in the neural network training dataset. For the supervised learning (SL) approach, real DAS instrumental noise measured on an acoustically isolated coil is added to synthetic data to generate training pairs of clean/noisy data. For the second method, the Noise2Noise (N2N) approach, the training is performed on noisy/noisy data pairs recorded simultaneously on the downgoing and upgoing parts of a downhole fiber-optic cable. Both approaches allow for the removal of unwanted noise that lies within the same frequency band of the useful signal, a result that cannot be achieved by conventional denoising techniques employing frequency filtering.
Optimizing Circular MIMO Array Imaging Using Partial Equivalent Method for Sidelobe Suppression
Authors: Dai Yiming , Zheng Zhikun , Ye Hong , Zhang Xu , Yang Jun , Deng Guangsheng , Li Ying , Yin Zhiping
Published: November 2024

In this paper, we propose a novel approach for circular Multiple-Input Multiple-Output (MIMO) array imaging, termed the Partial Equivalent Method (PEM), aimed at sidelobe suppression. In our method, the imaging process of the circular MIMO array is initially decomposed into bistatic circular synthetic aperture radar (BCSAR) components with different bistatic angles. Components with larger bistatic angles produce equivalent channels whose wavenumber spectra are concentrated near zero frequency, leading to significant broadening of the main lobe in the corresponding point spread function (PSF). In traditional MIMO imaging, each transmit–receive antenna pair is considered an equivalent channel, and all these channels are utilized for imaging. However, components with large bistatic angles, when integrated into the MIMO imaging output, result in increased sidelobe levels. To address this issue, we employ the PEM to restrict the range of equivalent channels. This method selectively retains effective channels generated by components with specific bistatic angles, effectively mitigating the adverse effects of BCSAR components with larger bistatic angles. Through point target simulations, electromagnetic simulations, and practical experiments, we demonstrate that the PEM significantly reduces sidelobes and enhances image quality in circular MIMO array imaging.