bluuebunny/crossref_metadata_2025 huggingface dataset:
Tissue-specific DamID protocol using nanopore sequencing
Authors: Gómez-Saldivar Georgina , A. Glauser Dominique , Meister Peter
Published: November 2022

DNA adenine methylation identification (DamID) is a powerful method to determine DNA binding profiles of proteins at a genomic scale. The method leverages the fusion between a protein of interest and the Dam methyltransferase of E. coli, which methylates proximal DNA in vivo. Here, we present an optimized procedure, which was developed for tissue-specific analyses in Caenorhabditis elegans and successfully used to footprint genes actively transcribed by RNA polymerases and to map transcription factor binding in gene regulatory regions. The present protocol details C. elegans-specific steps involved in the preparation of transgenic lines and genomic DNA samples, as well as broadly applicable steps for the DamID procedure, including the isolation of methylated DNA, the preparation of multiplexed libraries, Nanopore sequencing, and data analysis. Two distinctive features of the approach are (i) the use of an efficient recombination-based strategy to selectively analyze rare cell types and (ii) the use of Nanopore sequencing, which streamlines the process. The method allows researchers to go from genomic DNA samples to sequencing results in less than a week, while being sensitive enough to report reliable DNA footprints in cell types as rare as 2 cells per animal.
Perancangan PLTS Rooftop untuk Pemakaian Sendiri (PS) di PLTU Berau 2 × 7 MW
Authors: Nainggolan Irwan Firmanto , Windarta Jaka , Sinaga Nazaruddin
Published: November 2022

PLTU Berau 2 × 7 MW terletak di Kelurahan Teluk Bayur Kecamatan Teluk Bayur Kabupaten Berau Provinsi Kalimantan Timur yang Operation & Maintenance-nya di Kelola oleh PT. Indonesia Power. PLTU Berau 2 × 7 MW mensuplai energi listrik ke sistem jaringan isolated 20 KV Tanjung redeb. Konsumsi daya listrik (Pemakaian Sendiri) rata-rata sebesar 10.419.942,030 kWh/tahun. Salah satu program untuk menurunkan konsumsi pemakaian sendiri dan untuk mendukung kebijakan peningkatan Energi Baru Terbarukan (EBT) dalam bauran energi nasional hingga 23% pada tahun 2025 yaitu dengan pemasangan PLTS Rooftop. Kawasan PLTU Berau 2 × 7 MW memiliki nilai radiasi rata-rata sebesar 4,67 kWh/m²/hari sepanjang tahun 2020 dan rata-rata 3,9 kWh/m²/hari dalam rentang 22 tahun. Tujuan dari penelitian ini untuk mengetahui potensi energi surya yang dapat dihasilkan dilokasi Rooftop PLTU Berau 2 × 7 MW dengan menggunakan simulasi software HelioScope. Dari hasil simulasi diperoleh potensi energi listrik yang dapat dihasilkan adalah 570.364 kWh/tahun.
Humanising online teaching through care-centred pedagogies
Authors: Kızılcık Hale Hatice , Dewan Türüdü Aylin Selin
Published: November 2022

One of the main challenges regarding online teaching involves creating spaces in which learners establish and maintain connections with the teacher, other learners and the content. By exploring the concept of care in online learning, we aim to address this challenge. Framed by Noddings' (2013) ethics of care, this qualitative case study explored effective course design elements and instructional behaviours in an online course to identify practices that suggest the presence of care. Data collected through student feedback and peer observations provide insights into effective pedagogical practices, and our analysis reveals that these practices overlap with some of the four components of Noddings’ care-centred model of education: modelling, dialogue, practice, and confirmation. Our study suggests that what makes an online course effective might be the invisible care elements underlying its design and implementation.
Implications for practice and policy:

Online education experience can be enhanced by applying care-centred pedagogies into online settings to guide course design and instruction.
Educators can incorporate care principles into courses without putting excessive emphasis on emotions, which may be more preferable at higher education levels.
Wykłady o filozofii Plotyna
Authors: Kiedrowski Krzysztof
Published: November 2022

This book provides a brief introduction to the philosophy of Plotinus. It includes five lectures and an appendix containing an outline of Plotinus’ biography. The lectures aim to provide a synthetic yet analytical reconstruction of the main ideas of Plotinus’ system supported by relevant quotations from The Enneads. Lecture I (The Plotinian Universe) provides an overview of the relevant terminology and a presentation of the structure of the gradualist Plotinian universe. The relation of emanation/origin, which constitutes the main metaphysical relation in this universe, is also discussed in detail. Lecture II (The One) outlines the intellectual-historical context to which Plotinus’ philosophy refers (Parmenides’ Truth-being, Plato’s theory of the principles and the (neo)Pythagorean philosophy) and presents the way of understanding the One (the Absolute) through the interpretation of the terms used by Plotinus: ‘the One’, ‘the It’ and ‘the Good’. Lecture III (The Intellect) presents a discussion of two issues: the emanation of Intellect from the One and the nature of Intellect. The first issue is presented in the context of Plato’s theory of the principles, which is complemented by the hypothesis of return as contemplation, which is unique to the Plotinian system. The second part of the lecture is devoted to the characteristics of the noetic world (Intellect), as one-many and the Intellectual-Principle. Lecture IV (The All-Soul) contains a presentation of the concept of the origin of the Second Hypostasis (Soul) from the Intellect, its metaphysical properties, as well as a discussion of the generation of the World by the All-Soul, while lecture V (The Individual Soul) discusses the question of the multiplicity of Soul and the exceptional status of the human individual soul. A significant part of the lecture concerns the return of this soul to the Intellect (the paths of beauty, truth and virtue), as well as to the One (the mystical path, understood as intellectual insight).
Ideologie nieautorytarne – rozwój jednostki a edukacja demokratyczna
Authors: Michałowska Danuta Anna
Published: November 2022

Some selected issues from the philosophy of education concern ideologies of education, especially the non-authoritarian ones indicated by Roland Meighan, the concept of person-centred education, and education for democracy. The aim of the analyses is an attempt to define a model of education that will foster young people’s creative development, respecting their autonomy and, in parallel, that will begin building a democratic society already at the stage of student communities. The ideology of autonomously learning the concept of autonomy, the principles of self-regulation in the learning process, the specific roles of the teacher and the student, as well as the style of work and strategies for acquiring knowledge and skills – all these issues are discussed in the context of the problem of achieving the goals of democratic education. The issue of individual freedom and autonomy in education is analysed and, drawing on the example of an English independent school, the features of the educational process which lead to the realization of most of the assumptions of non-uthoritarian ideology and a flexible approach to teaching are described. In the ‘learner-centred school’ model of Donna Brandes and Paul Ginnis, based on the humanistic approach to education developed by Carl Rogers and Fritz Perls, the autonomously developing individual has the opportunity to experience the limits of freedom, responsibility for their choices and for the quality of relationships, as well as cooperation and community building based on democratic principles. The analysis of the democratic model of education and school practices indicates the possibilities for building democratic student communities while at the same time providing conditions for the comprehensive development of the person and their autonomy. It is a philosophy of education focused on the person – one who belongs a community of autonomous individuals.
On the crossroads: Findings of the Late Neolithic figural plastics in Petrijevci (Eastern Croatia)
Authors: Burić Marcel
Published: November 2022

During the rescue excavations in the periphery of the village Petrijevci (Eastern Croatia), a set of Neolithic figural plastic findings was discovered. The group of findings consists of six fragments of “Vinča-like” type figurines, discovered in the Sopot cultural area. In the paper, an attempt of cultural and typological determination will be presented, with some references to the question of the Late Neolithic figural plastics in Croatia.
Investigating Flow in Motorcycle Tourism: A Review of Previous Research and Identification of Opportunities
Authors: Frash Jr. Robert E. , Blose Julia E.
Published: November 2022

This paper explores whether the psychological construct of flow plays an important role in shaping motorcycle tourism behavior and examines the relative success of methodologies previously used to model flow, in general. The systematic quantitative literature review (SQLR) method was applied. It is concluded that flow, the mental state, is likely a meaningful intrinsic motivator of motorcycle tourism. Limitations related to previous efforts to model this phenomenon are identified. Suggestions for future research on the role of flow in the motorcycle tourism context are provided with domain-specific methodological strategies outlined.
Neue Vielfalt der Regelgeber und Regelungstechniken im Gesellschafts- und Kapitalmarktrecht unter Einschluss des Bilanzrechts
Authors: Binder Jens-Hinrich
Published: November 2022

Im Zusammenhang mit dem Aufwuchs von Rechtspflichten und Standards zu ökologischen und sozialen Implikationen unternehmerischen Handels hat sich in jüngerer Zeit das Spektrum der Regelgeber und Regelungstechniken im Gesellschafts-, Kapitalmarkt- und Bilanzrecht erheblich erweitert. Insbesondere kapitalmarktorientierte Aktiengesellschaften werden dabei im deutschen und europäischen Recht zunehmend zur Einhaltung internationaler, von nichtstaatlichen Regelsetzern erarbeiteter Rahmenwerke veranlasst. Auf den ersten Blick knüpft die Entwicklung an das bereits seit den frühen 2000er Jahren im deutschen Unternehmensrecht etablierte Konzept der indirekten Regulierung durch Publizität nichtfinanzieller Informationen an. Der Beitrag geht der Frage nach, wie sich die „neue Vielfalt“ der Regelgeber und Regelungstechniken von diesen Vorläuferentwicklungen unterscheidet, und diskutiert mögliche Konsequenzen für Unternehmensverfassung und Organpflichten.
Neue Organisationsformen für die digitale Welt? Decentralized Autonomous Organizations and Beyond
Authors: Zetzsche Dirk
Published: November 2022

Dieser Aufsatz untersucht die Anwendung des deutschen Rechts auf neue dezentrale Unternehmensformen, wie sie als „DAO“ oder im Metaverse anzutreffen sind, und erarbeitet Vorschläge de lege ferenda zur Schaffung einer neuen Gesellschaftsform für dezentral organisierte Unternehmen. Die Anwendung des deutschen Gesellschaftsrechts führt zur Qualifikation als GbR oder nicht eingetragener Verein. Dabei qualifizieren nur Gründer-Entwickler sowie die ‚nodes“ als Mitglied bzw. Gesellschafter. Indirekte Partizipation über Wallets genügt nicht. Eine de lege ferenda zu schaffende Rechtsform muss den konkreten Dezentralisierungsgrad der Einflussmöglichkeiten berücksichtigen, wobei eine vollständige Dezentralisierung ein Mythos ist. Eine Rechtsform könnte daher nach Art einer KG gestaltet sein, mit den Gründer-Entwicklern und anderen Personen mit Code-Zugriff als Komplementären und nur-Nodes als Kommanditisten. Zugleich wird gezeigt, dass einer Ordnung dieser innovativen Unternehmensformen über das Gesellschaftsrecht die Alternative einer funktionsbezogenen Ordnung über das Vertrags- und Deliktsrecht gegenübersteht.
Theoretical study on the origins of sildenafil tautomers’ relative stability
Authors: Pajka Dariusz , Lelek-Borkowska Urszula , Zborowski Krzysztof K.
Published: November 2022

Tautomers of sildenafil (5-{2-Ethoxy-5-[(4-methylpiperazin-1-yl)sulfonyl]phenyl}-1-methyl-3-propyl-1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one), a drug commonly used for treating the erectile dysfunction and pulmonary arterial hypertension were studied computationally at the DFT (B3LYP/6-311++G**) level. Four possible structures of sildenafil tautomers structures were considered. The geometries and chemical shifts of carbon and nitrogen nuclei were calculated and compared with available experimental data. The theoretical calculations proved to be consistent with experimental data and indicate the same lowest energy sildenafil structure. The energies of the all considered sildenafil tautomers were determined and ranked according to their energy values. This article presents calculations of several properties (aromaticity, atomic charges, atomic energies, intramolecular hydrogen bonding) and discusses their influence on the relative energies of sildenafil tautomers.
The Neutrophil/Lymphocyte Ratio is Associated with Different Stages of Development of Coronary Artery Disease
Authors: Du Jun , Deng Songbai , Du Jianlin
Published: November 2022

Introduction: Inflammation plays a role in coronary artery disease (CAD). The neutrophil/lymphocyte ratio (NLR), monocyte/lymphocyte ratio (MLR) and platelet/lymphocyte ratio (PLR) are blood parameters associated with inflammation. We aimed to perform a systematic comparison and study the predictive values of these inflammatory parameters with respect to CAD stage.

Method: A total of 513 patients who had undergone coronary angiography (CAG) were retrospectively analyzed. Clinical status, lipid profiles, CAG scans and hematological parameters were collected. NLR, MLR and PLR were calculated. All patients were classified into a normal coronary group (n=133), coronary atherosclerosis (CA) group (n=149), chronic coronary syndrome (CCS) group (n=175) or acute myocardial infarction (AMI) group (n=56). NLR, MLR and PLR were compared among groups.

Results: NLR and MLR were higher in the CCS and AMI groups. PLR was higher in the AMI group. The AMI group had higher NLR, PLR and MLR than the CCS group. Logistic regression analysis revealed that NLR (OR:1.227, 95% CI: 1.016–1.482. P<0.05) had a strong significant correlation with CAD. Area under the ROC curve of NLR was 0.580 (95% CI=0.516–0.644) in predicting CCS and 0.727 (95% CI=0.642–0.811) in predicting AMI.

Conclusion: NLR may be associated with the occurrence and progression of CAD, and may serve as a marker of inflammation.
Linear type trait genetic trends in Irish Holstein-Friesian dairy animals
Authors: Berry D.P. , Ring S.C. , Kelleher M.M.
Published: November 2022

The objective of the present study was to investigate the genetic trends of 18 subjectively scored linear type traits describing animal morphology, as well as udder, teat, feet and leg conformation. The analysis was undertaken using 2,932,700 Holstein-Friesian females born in the Republic of Ireland between the years 2000 and 2020, inclusive. The results indicate that Holstein-Friesian females have progressively become shorter in stature as well as shallower (i.e. body depth) and less angular. The reduction in genetic merit for stature score since the year 2004 was, however, only observed in non-herdbook-registered heifers. Furthermore, the reducing score in body depth (i.e. narrower) and angularity (i.e. less angular) was approximately twice as fast in non-herdbook-registered heifers as it was in herdbook-registered heifers. Differences in the genetic merit of the body-related traits for calves born versus those that became cows only existed prior to 2010 with little biological differences thereafter; this observation was common across most of the linear type traits. Genetic merit for locomotion in non-herdbook-registered animals has deteriorated over the 20-yr period, while the foot angle over that period is becoming lower; no such trends were observed for the herdbook-registered animals. Large differences not only in the trends themselves, but also in the mean genetic merit for udder traits existed when comparing herdbook-registered calves versus non-registered calves. In conclusion, genetic merit for many of the traits evaluated has trended relatively consistent in a given direction, albeit the cumulative change in genetic s.d. units per traits over the 20-yr period was very small.
An incentive system for decentralized DAG-based platforms
Authors: Mazurok Igor Y. , Leonchyk Yevhen Y. , Grybniak Sergii S. , Nashyvan Oleksandr S. , Masalskyi Ruslan O.
Published: November 2022

Decentralized public platforms are becoming increasingly popular due to a growing number of applications for various areas ofbusiness, finance, and social life. Authorless nodes can easily join such networks without any confirmation, making a transparent system of rewards and punishments crucial for the self-sustainability of public platforms. To achieve this, a system for incentivizing and punishing Workers’ behavior should be tightly harmonized with the corresponding consensus protocol, taking into account all of its features, and facilitating a favorable and supportive environment with equal rights for all participants. The main purpose of re-wards is to incentivize Workers to follow the protocol properly, and to penalize them for any type of misbehavior. The issues of block rewarding and punishing in decentralized networks have been well studied, but the DAG referential structure of the distributed ledger forces us to design methods that are more relevant. Since referential structures cannot be reliably validated due to the fact that they are built on the basis of the instantaneous visibility of blocks by a certain node, we propose to set rewards for blocksin the DAG network based on the degree of confidence of topological structures. In doing so, all honest nodes make common decisions based only on information recorded into the ledger, without overloading the network with additional interactions, since such data are al-ways identical and available.The main goal of this work is to design a fair distribution of rewards among honest Workers and estab-lish values for penalties for faulty ones, to ensure the general economic equilibrium of the Waterfall platform. The proposedap-proach has a flexible and transparent architecture that allows for its use for a wide range of PoS-based consensus protocols. The core principles are that Workers' rewards depend on the importance of the conducted work for block producing and achieving consensusand their penalties must not be less than the potential profit from possible attacks. The incentivizing system can facilitate protection from various kinds of attacks, namely, so-called Nothing-at-stake, Rich-get-richer, Sybil, and Splitting attacks, and from some specif-ic threats related to a DAG structure.
On the cascade GL-model and its properties
Authors: Romankevitch Alexei M. , Morozov Kostiantyn V. , Mykytenko Serhii S. , Kovalenko Olena P.
Published: November 2022

The article proposes a new direction for the further development of GL-models–models on the basis of which performs the calculation of the reliability parameters of fault-tolerant multiprocessor systems. Such models reflect the reaction of the system to the appearance of failures of arbitrary multiplicity.The essence of the new direction is the construction of a model by composition of several basic GL-models in such a way that the values of the edge functions of onemodel form the input vector of the next one. This article shows that the model obtained in this way, which is proposed to be called cascade model, will also be basic and, in general case, can consist of an arbitrary number of submodels. This article givesa formula that allows one to determine the value of the degree of fault tolerance of the cascade model, depending on the values of the levels of fault tolerance of its component submodels. This article shows that the graphs of both the cascade and regularmodels are cyclic and have the same number of edges. At the same time, despite the fact that the intermediate submodels also have graphs, their presence does not increase the complexity of the model as a whole, since only the expressions of the edge functions are used in them. This article contains examples that confirm the correctness of the theoretically obtained results, and it also shows that the cascade model, at least in some cases, has lower computational complexity (the total number of logical operations in the expressions of edge functions) compared to the basic model. It was found that although the cascade model is basic, the sets of edges itloses and the regular basic GL-model on some input vectors may differ. In certain cases, several alternative cascade models can be built, which will differ in their parameters, but will have the same resulting value of the degree of fault tolerance.Given an example, where the properties of such alternative cascade models are compared. It was found that such models differ both in computational complexity and, in some cases, in the sets of edges they lose on certain input vectors. The possibility of modifying the cascade model was shown by changing the expressions of the edge functions of its component submodels, both individually and several simultaneously. At the same time, it is possible to block vectors with an increased multiplicity of zeros. A number of tasks for future research wereformulated.
The study of the quality of multi-step time series forecasting
Authors: Tishyn Petr M. , Buyukli Victor S.
Published: November 2022

The work is devoted to the study of the quality of multistep forecasting of time series using the electricity consumption data for forecasting. Five models of multistep forecasting have been implemented, with their subsequent training and evaluation of the results obtained. The dataset is an upgraded minute-by-minute measurement of four years of electricity consumption. The dataset has been divided into training, validation, and test samples for training and testing models. The implementation is simplified by using the TensorFlow machine learning library, which allows us to conveniently process and present data; build and train neural networks. The TensorFlow functionality also provides standard metrics used to assess the accuracy of time series forecasting, which made it possible to evaluate the obtained models for forecasting the time series of electricity consumption and highlight the best ofthose considered according to the given indicators. The models are built in such a way that they can be used in studies of the quality of time series forecasting in various areas of human life. The problem of multistep forecasting for twenty fourhours ahead, considered in the paper, has not yet been solved for estimating electricity consumption. Theobtainedforecasting accuracy is comparable to recently published methods for estimating electricity consumption used in other conditions.At the same time, the forecasting accuracy of the constructed models has been improved in comparison with other methods.
Developing of functioning algorithm ofinformation-control system for phase-shifting devices in power lines
Authors: Savieliev Artem A.
Published: November 2022

The article is devoted todevelopment of afunctioning algorithm of the control systemof phase-shifting devices in double-circuit overhead power lines, as well as to the formulation of the main tasks of this system. Based on these, functional schemes for controllingphase-shifting devices were developed.The developed system will allow, through the use of phase-shifting transformers, which belong to the flexible alternating currenttransmission system (FACTS)technology, to change the value of phase shift angle of the voltage and current vectors of one circuit relative to anotherthat.Thiswill lead to a change in the value of the wave impedance of double-circuit lines, due to the emergence of new electromagnetic connections between the conductors of the two circuits. The main task of the developed system is to support the operation of power lines in the matched load mode, when the value of the wave imped-anceis equal to the value of the load impedance. It follows from the analysis that this mode provides the lowest losses in the trans-mission of electricity through power lines.The implementation of this control system is planned by using the Internet of Things in-formation technology, that is, the formation of a wireless connection between the main elements of the system for data transmission.The developed system can be attributed to information systems, as well as the current concept of SmartGrid.Thus, a method has been proposed to increase efficiency by reducing losses during the transmission of electricity through double-circuit power lines through the use of the concept of Smart Grid.
New Age of Indonesian Local Tourism Development: Community Bonding, Youth Work, and Selling Tourism
Authors: Hatma Indra Jaya Pajar , Izudin Ahmad , Aditya Rahadiyand
Published: November 2022

This article explores why and how youth can build community bonds in the new era of rural development in Indonesia that produces local works in the form of tourist sales products. This article uses qualitative methods through a case study approach. The research was conducted in Balkondes Wringin Putih. Starting from individual unrest into community bonds that produce youth solidity, the village succeeded in developing tourist destinations in special economic areas based on tourism. When tourism development programs become the country’s priority, youth through the provision of village tour packages can create new jobs and increase rural economic productivity. The community ties and administration in the village’s growth, resulting from the ruralization process, are contextualised in the macro-political structure of the new age of Indonesian rural development, in fact, able to develop youth-based economic resilience. As empirical research to understand the development of new rural tourism, this study learns from the practices in Balkondes about a relational view of the ever-changing mechanisms of community bonding in the modern world.
Sociology of Space: The Elderly Activity Space Before and During the Covid-19 Pandemic
Authors: Wijayanti Wijayanti , Lestari Ria Ripardi Wahyu
Published: November 2022

The current pandemic has an impact on all lines of life, especially on elderly people with vulnerable or declining health status. Prior to the COVID-19 pandemic, the elderly were active in activities inside and outside their homes to fill their spare time in their old age, but during social restrictions and physical distancing, they were forced to stay in their homes for an indefinite time. Through qualitative methods, this research explores the activity space of elderly women who are still active in daily activities both at home and in their neighborhood before the pandemic and during the pandemic where government policies limit/influence the activities of the elderly. Participants of the research are 60 years old elderly women and over (WHO, 2020) who live in Banyumanik residence. The results showed that there was a decrease in the scale of the activity space used by the elderly during the pandemic, namely from the environmental space scale to the residential space scale.
Pesantren Al-Fattah: The Strugle of an Ascetic Waria to Create a Religious Space for Transgender Muslims in Indonesia
Authors: Nila Sari Maesa , Alimi Moh Yasir , Robinson Kathryn
Published: November 2022

This study seeks to describe the struggle of a Muslim transgender ascetic in claiming religious space for transgender in urban Indonesia by creating a pesantren (Islamic school). The study was conducted in Al-Fattah Pondok Pesantren, the only Islamic school for transgender in Indonesia. Al-Fattah is created by Bu Maryani, a transgender ascetic, who contributed her money, thought, energy and prayers for this school, to provide spiritual sanctuary for transvestites, despites her worsening economy and health. While many transvestites look for alternative religion, the experience of Al-Fattah reveal that transgender Muslims may find their religious sanctuary within traditional Islamic practices, not outside the traditional institutions. Al-Fattah transgender Islamic school reveal the power of lived religion, pesantren, and traditional forms of religious rituals to validate Queer spiritual practices. We argue that the use of the traditional forms of religious rituals enable the Muslim transgender to overcome the dilemma of fluidity-and-fixity in queer religious practices.
Testis and brown adipose tissue xenografts from yellowish myotis (Myotis levis)
Authors: Farias Talita De Oliveira , Figueiredo André Felipe Almeida , Wnuk Natalia Teixeira , Talamoni Sônia Aparecida , Costa Guilherme Mattos Jardim
Published: November 2022

Yellowish myotis present a seasonal reproduction, influenced by rainfall distribution, in which the testis mass, germ cell composition, and brown adipose tissue (B.A.T.) mass change along the reproductive stages. In the present study, tissue xenografts were performed in immunodeficient mice to investigate spermatogenesis development in a stable endocrine milieu and the possible androgenic role of B.A.T. In this study, 41 adult male bats were captured in the Santuário do Caraça, Minas Gerais, Brazil. The gonads and B.A.T. were collected, weighed, and grafted under the mice's back skin. Mice biometric and hormonal data were evaluated after grafting, and the testis grafts and mice gonads were fixed for histological and immunohistochemical analyses. As a result, testis grafts from adult bats presented a continuous germ cell development in all reproductive stages, showing round spermatids in all testis tissues. Furthermore, testis fragments in the Rest stage presented elongating spermatids as the most advanced germ cell type in the seminiferous epithelium after 7 months of grafting. These data indicated that yellowish myotis spermatogenesis could be continued (presenting a constant spermatogonial differentiation) in a stable endocrine milieu, as found in mice. In addition, the best spermatogenic development was achieved when testis fragments were transplanted at their lowest activity (Rest stage). Regarding the B.A.T. grafts, the adipose tissue consumption by mice increased seminal vesicle mass and testosterone serum levels. This data proves that B.A.T. is related to testosterone synthesis, which may be critical in stimulating the differentiation of spermatogonia in yellowish myotis.

Lay summary

Bats are essential seed dispersers, pollinators, and agricultural pest regulators. Despite their ecological importance, bats face different threats due to environmental destruction and usually have few offspring per year. This study aimed to understand better how bats reproduce, but studying them in captivity is complicated and may not replicate what happens in the natural environment. To overcome this obstacle, we transplanted tissues from bats into mice which allowed in-depth research in lab conditions into bat reproduction. We looked at the tissues of adult bats after they had been transplanted into mice, and this allowed us to see which types of tissue played a critical role in reproduction.
РОЗВИТОК КРИТИЧНОГО МИСЛЕННЯ МОЛОДШИХ ШКОЛЯРІВ У ПРОЦЕСІ ВИВЧЕННЯ ПРЕДМЕТІВ МОВНО-ЛІТЕРАТУРНОЇ ОСВІТНЬОЇ ГАЛУЗІ
Authors: OLIIAR MARIIA
Published: November 2022

Стаття присвячена проблемі розвитку критичного мислення молодших школярів у процесі вивчення предметів мовно-літературної освітньої галузі. Подано трактування українськими вченими критичного мислення учня. Розкрито можливості предметів мовно-літературної освітньої галузі у формуванні критичного мислення молодших школярів. Зазначено, що одним із дієвих засобів формування критичного мислення дітей є їхня мовленнєва творчість. Висвітлено прийоми і технології розвитку критичного мислення молодших школярів на основі мовного матеріалу. Описано структуру організації творчої діяльності учнів задля формування їхнього критичного мислення.
ФУНДАМЕНТАЛЬНІ ПРИНЦИПИ НОВОЇ УКРАЇНСЬКОЇ ШКОЛИ У ПРАЦЯХ ГРИГОРІЯ СКОВОРОДИ, КОСТЯНТИНА УШИНСЬКОГО І ВАСИЛЯ СУХОМЛИНСЬКОГО
Authors: SIKORSKYI PETRO
Published: November 2022

У науковій статті досліджується науково-педагогічна спадщина відомих українських педагогів Г. Сковороди, К. Ушинського і В. Сухомлинського в частині фундаментальних принципів педагогіки (природовідповідності, нерівної рівності, розвивального навчання). Аналізуючи розвиток педагогічних поглядів учених щодо природовідповідного, розвивального навчання, автор інтегрує і поглиблює їх, а також дає цим принципам наукове визначення. Зазначається, що між принципами природовідповідності, розвивального навчання, нерівної рівності існують взаємозв’язок і взаємодія. Автор акцентує, що принцип нерівної рівності (Г. Сковорода) досі не був зактуалізованим в українській педагогіці, а саме він дозволяє стверджувати, що за ніяких, навіть ідеальних умов навчання, не вдасться досягти високих навчальних досягнень з усіма учнями. Доведено, якщо цей принцип ігнорувати, то в навчанні учнів домінуватимуть авторитарні методи навчання, тобто школа не буде виконувати однієї зі своїх основних функцій: виховувати й усебічно розвивати учнів. Наголошується, що вище зазначені принципи особливо актуальні в сучасних умовах, коли системно реформується середня освіта за концепцією «Нова українська школа».
ВИКОРИСТАННЯ ІННОВАЦІЙНИХ МЕТОДИК ФІЗИЧНОГО ВИХОВАННЯ У СУЧАСНОМУ ЗАКЛАДІ ДОШКІЛЬНОЇ ОСВІТИ
Authors: MOSEICHUK YURII , TSYBANUYK OLEKSANDRA
Published: November 2022

У статті проаналізовані необхідність і вагомість використання інноваційних методик фізичного виховання дітей у сучасних закладах дошкільної освіти на прикладі їзди на самокаті та флорболу. Автори доводять, що  впровадження у практику роботи різноманітних інноваційних здоров’язбережувальних технологій (фітбол аеробіка, степ аеробіка тощо; пальчикову, дихальну гімнастику, гімнастику для очей, елементи хатха-йоги тощо) – один з пріоритетних напрямів роботи дошкільного навчального закладу. Важливо, що у статті визначений вік, з якого рекомендовано займатись цією вправою, обґрунтований алгоритм вивчення безпосередньо їзди на самокаті у фізичному вихованні дошкільника. Надані рекомендації щодо поетапного опанування знаннями, уміння та навичками вільного прокачування, гальмування, виконання поворотів і зупинок тощо.
ФОРМУВАННЯ НАВИЧОК ПОБУДОВИ ОБ'ЄМНО-ПРОСТОРОВОЇ КОМПОЗИЦІЇ У МАЙБУТНІХ ФАХІВЦІВ МИСТЕЦЬКИХ СПЕЦІАЛЬНОСТЕЙ У ХОДІ ОЗНАЙОМЛЕННЯ З ПАРКОВИМИ СТРУКТУРАМИ
Authors: KHALAYTSAN VOLODYMYR
Published: November 2022

У статті досліджено потенційні можливості задіяння паркових ансамблів Поділля в ролі об'єктів вивчення, баз пленерної практики, взірців проєктно-дизайнерської діяльності в ході опанування навичок побудови об'ємно-просторової композиції. Лаконічно описано структурні характеристики парків, визначено їх унікальність. З'ясовано, що в паркових структурах Поділля традиційно міститься атрибутика, притаманна паркам епохи романтизму, гармонійно поєднана з особливостями рельєфу в місцевих геокліматичних умовах. Унікальність полягає в підходах до планувальної діяльності у формуванні композиції парку. Ознайомлення із зазначеними композиційними побудовами сприятиме професійному зростанню майбутніх фахівців у галузі мистецтва.
Synthesis and properties of VPO catalysts for oxidation of n-butane to maleic anhydride
Authors:   Institute for Sorption and Problems of Endoecology of the National Academy of Sciences of Ukraine, Diyuk O.A.
Published: November 2022

Bulk and supported vanadium-phosphorus oxide VPO catalysts were synthesized by traditional and barothermal methods. It was shown that the use of aerosil as a support for the VPO phase, depending on the time of its introduction into the reaction mixture. It can lead to the formation of catalyst precursor of vanadyl hydrogen phosphate VOHPO4 0.5H2O, or a phase of vanadyl pyrophosphate (VO)2P2O7 as already the catalytically active phase for selective oxidation of n-butane to maleic anhydride. The use of a modified aerosil gel formed from pyrogenic aerosol, as a support for the VPO phase, leads to the formation of VOHPO4∙0.5H2O phase. It has been found that the nature of support affects the features of formation of VOHPO4∙0.5H2O phase, in particular, the ratio of crystallographic planes in resulting VPO phase. The use of aerosil as a support leads to a decrease in the relative content of the basal plane, while use of aerosil gel leads to an increase in the relative content of the basal plane in applied VPO phase. The catalytic properties of bulk and supported VPO samples were studied in the selective oxidation of n-butane to maleic anhydride in standard (1.7 vol.%) and enriched (3.4 vol.%) n-butane mixtures. It has been found that in an enriched n-butane mixture for bulk samples, the n-butane conversion and selectivity for maleic anhydride are sharply reduced. It has been found that supported VPO samples have a higher specific rate of n-butane oxidation and higher productivity compared to bulk samples. It was shown that use of barothermal synthesis and aerosol gel as a support made it possible to increase the selectivity of maleic anhydride, which is associated with an increase in the relative content of the basal plane of VPO phase. The achieved improved catalytic properties of VPO catalysts supported with aerosol gel make recycling technology promising. This can make the production of maleic anhydride more economical.
Catalytic processing of the acid tars
Authors:   National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Krymets G.V. , Litynska M.I. , Melnychuk O.V. ,   V.P Kukhar Institute of Bioorganic Chemistry and Petrochemistry of NAS of Ukraine
Published: November 2022

Acid tars are wastes from the processing of coal, petroleum, and petrochemicals (oil refining, benzene refining and petroleum fractions refining and alkylation of isobutane with butenes). Acid tar compositions include resinous substances, organic matter, and polymerization products of unsaturated hydrocarbons. The presence of free sulfuric acid in acid tars often reaches 70 % by weight. Almost all metals from oil are concentrated in tars, and the content of vanadium and nickel can reach 0.046 and 0.014 %, respectively. A lot of countries keep acid tar in the open air in spent quarries, storage ponds, barns, lagoons or near landfills. It poses a risk or even potential threat to people and to the environment nearby due to soil, water, and air pollution. Thus, disposal of the acid tars is a very important ecological and industrial task. In this study, we have researched catalytic cracking and distillation as the utilization methods for acid tar. Anhydrous AlCl3 was used as a catalyst during the cracking of petroleum residues to obtain volatile gasoline fractions due to its catalytic activity in many organic reactions. The catalyst ratios (0.15 g/g of tar or 0.1 g/g of tar) had a very significant influence on the number of volatile fractions and boiling temperature in the acid tar cracking process. According to the results of 1H NMR research, the main components of volatile fractions in the case of catalytic cracking were alkanes CH3-(CH2)n-CH3. The compositions of these fractions were similar to the compositions of gasoline and diesel fuel. A series of distillation experiments (distillation of previously deacidified and centrifuged tar, acid tar without deacidification and centrifugation, and previously deacidified tar without centrifu-gation) gave different results for each type of material. Aliphatic hydrocarbons were the main components of volatile fractions (~ 80, ~ 60 and ~ 90 %, respectively) and the contents of aliphatic S-organic compounds were also significant (~ 10, ~ 30 and ~ 8 %). Thus, both for catalytic cracking and for tar distillation, aliphatic hydrocarbons were the main component of volatile fractions. Deacidification of tar increased the yield of aliphatic hydrocarbons during tar distillation and decreased production of S-organic compounds due to its reactions with calcium carbonate. It is perspective in the context of fuel production.
Performance and 12-month Outcomes of a Wire-free Fractional Flow Reserve System for Assessment of Coronary Artery Disease
Authors: Bhavnani Chandan Deepak , Fong Alan Yean Yip , Koh Keng Tat , Pang Ing Xiang , Chen Lean Seng , Ling Hwei Sung , Thien Lee Karl , Khiong Chung Bui , Tan Chen Ting , Oon Yen Yee , Ho Kian Hui , Shu Francis Eng Pbeng , Said Asri , Cham Yee Ling , Ong Tiong Kiam
Published: November 2022

Background: Fractional flow reserve (FFR) using an invasive pressure wire is recommended to guide coronary revascularisation in stable coronary artery disease. Coronary angiography-based wire-free FFR (CAFFR) determines the significance of a coronary lesion without the requirement of a pressure wire. Deferral of revascularisation of coronary lesions with an FFR >0.8 has been shown to have similar outcomes to patients managed with optimal medical therapy. Objective: The aim of our study was to assess the performance and 12-month clinical outcomes in patients with CAFFR-guided percutaneous coronary intervention (PCI) deferral. Methods: This was a prospective study involving 69 patients (93 vessels) with angiographic stenosis of 30–90%. Patients with CAFFR ≤0.80 or poor image quality were excluded, leaving 29 patients (31 vessels) for analysis. All recruited patients had a CAFFR >0.80 and thus, PCI deferral. This cohort was followed up for 12 months. The primary endpoint was a composite of death from any cause, MI or target vessel revascularisation. Wired FFR was done for comparison on 14 patients (48%) at the operator’s discretion. Results: The mean age was 59.9 (±12.6) years. The majority of patients were men (83%; n=24), 41% (n=12) had diabetes, 62% (n=18) had hypertension, 59% (n=17) had dyslipidaemia, 62% (n=18) had a history of smoking. The mean left ventricular ejection fraction (LVEF) was 52 (±11.4)% and 76% of the patients had a recent acute coronary syndrome. We assessed the left anterior descending artery and 52% (n=16) of vessels had a mean CAFFR was 0.87. At 12 months, all patients were alive, 89.7% remained in chronic coronary syndrome (CCS) class 1 and 3.4% (n=1) of the study population met the primary outcome of target vessel revascularisation. Conclusion: CAFFR showed good agreement with wire-based FFR and 12-month outcomes showed that CAFFR-guided deferral of PCI was safe and comparable to wired-based FFR guidance.
Ön çapraz bağ rekonstrüksiyonu sonrası verilen dirençli egzersiz eğitiminin sıçrama performansı üzerine etkisi
Authors: UZUN Ayşe , ERGUN Nevin , ARSLAN Damla , BALTACI Gül , YAKUT Yavuz
Published: November 2022

Amaç: İzokinetik kas kuvveti ve tek bacak sıçrama testi diz cerrahileri sonrasında spora veya günlük yaşama dönüşte bireyleri değerlendirmek için sıklıkla kullanılan ölçümlerdir. Bu çalışmanın amacı verilen izokinetik eğitimin, hamstring ve quadriceps kas kuvveti üzerindeki etkinliğini tek bacak sıçrama testi kullanarak değerlendirmek idi. Yöntem: Ön Çapraz Bağ Rekonstrüksiyonu geçiren 20 erkek hasta çalışmaya dahil edildi. 16. ve 24. haftalarda izokinetik kas kuvveti ve tek bacak sıçrama testleri yapıldı ve bu haftalar arasında bireylere izokinetik eğitim verildi. Zirve tork quadriceps/vücut ağırlığı, zirve tork hamstring/vücut ağırlığı, hamstring/quadriceps oranı değerleri çalışmaya dahil edildi. İkili karşılaştırmada Wilcoxon testi, korelasyon analizinde Pearson korelasyon katsayısı kullanıldı. Bulgular: Opere dizde 16. haftasında, ekstansiyon zirve tork değeri ile tek bacak sıçrama testi arasında orta derecede ilişki görülürken (r=0,622, p=0,023), sağlam dizde, ekstansiyon zirve tork değeri ile tek bacak sıçrama testi arasında bir ilişki görülmedi (r=0,147, p=0,631). 24.haftada, opere ve sağlam dizdeki ekstansiyon zirve tork değeri ve tek bacak sıçrama testi arasındaki ilişki orta derece bulundu (r=0,595, p=0,032), (r= 0,591, p=0,033). 16. haftada hamstring/quadriceps oranı ile tek bacak sıçrama testi arasındaki ilişki yüksek bulundu (r=0,725, p=0,005). Sonuç: Çalışmanın sonucunda, ön çapraz bağ rekonstrüksiyonu sonrası verilen dirençli izokinetik eğitimin, tek bacak sıçrama performansını orta derecede etkilediği bulundu.
Abordaje laparoscópico exitoso de lesión vesical intraperitoneal ocasionada durante una cirugía ginecológica. Reporte de caso
Authors: Castañeda Millán David Andrés , Abonia Velasco Carlos Hernán , Álvarez Restrepo Juan Camilo , Buitrago Carrascal Christian , Ángel Muller Edith , Donoso Donoso Wilfredo
Published: November 2022

Introduction: Urinary tract injuries are common in the context of pelvic surgery; however, there is little information in the medical literature on the management of these injuries using a laparoscopic approach. The following is the clinical case of a patient with intraperitoneal bladder injury secondary to gynecological pelvic surgery that was successfully treated using a laparoscopic approach. Moreover, a management algorithm is proposed based on the scientific data available.
Case report. A 39-year-old female patient was admitted to the hospital with generalized abdominal pain and anuria three days after undergoing a left laparoscopic salpingectomy and resection of a migrated intrauterine device. Laboratory tests showed microhematuria, serum creatinine of 4.35mg/dL, and urea nitrogen of 35.2mg/dL. Computed tomography cystography showed a solution of continuity on the left posterolateral intraperitoneal bladder. The patient underwent successful laparoscopic treatment for the bladder injury, and two weeks later, retrograde cystography confirmed its adequate resolution.
Conclusions. Based on the benefits of the laparoscopic approach and after extrapolating the outcomes of the laparoscopic management of bladder trauma, it can be concluded that the laparoscopic approach to surgical injuries of the bladder is a feasible therapeutic option.
Adenoma metanéfrico: diagnóstico diferencial del carcinoma urotelial del tracto urinario superior. Reporte de un caso
Authors: Castañeda Millán David Andrés , Álvarez Restrepo Juan Camilo , Romero Nieto Víctor Iván , Camacho Nieto Diego , Donoso Donoso Wilfredo , Forero Muñoz Jorge
Published: November 2022

Introduction: Metanephric adenoma is a rare benign kidney tumor. Patients with these tumors are usually asymptomatic, although polycythemia occurs in up 12% of cases. These masses are often described on diagnostic imaging as solid, single, well-defined, oval-shaped, unilateral lesions, located primarily in the renal medulla and without extrarenal involvement. These neoplasms are difficult to differentiate from malignant neoplasms of the upper urinary tract, so the definitive diagnosis is achieved by histopathology. Currently, the treatment of choice is radical nephrectomy.
Case presentation: A 51-year-old woman from Bogotá (Colombia) was referred to the urology service of a tertiary care hospital due to bilateral lumbar pain of non-specific characteristics. At the time of consultation, the patient was asymptomatic. Renal and urinary tract ultrasound showed hydronephrosis and right renal mass. Computed tomography urography was requested, which revealed a lesion in the right renal pelvis with parenchymal invasion highly suggestive of high-risk upper urinary tract urothelial carcinoma, as well as adenopathies in the para-aortic lymph nodes. The patient underwent a radical nephroureterectomy with bladder cuff, which allowed establishing a final diagnosis of metanephric adenoma according to the histopathological study.
Conclusions: Metanephric adenoma is a rare tumor that is difficult to diagnose through imaging, so it is necessary to explore additional tools to establish an accurate pre-surgical diagnosis that allows preserving the affected renal unit. Also, given their non-specificity, these tumors should be included in the differential diagnosis of lesions suggestive of upper tract urothelial carcinoma.