bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalencia de adiposidad corporal y dislipidemia en funcionarios de la Policía Nacional Bolivariana de la Universidad Nacional Experimental de la Seguridad
Authors: Corvos Hidalgo Cesar Augusto , Corvos Andrea Victoria , Hidalgo Rafael Ramón
Published: December 2018

Introducción: La composición corporal es uno de los elementos clave en el desarrollo de la labor operacional del funcionario policial, que, con un elevado porcentaje de adiposidad corporal, resultarían muy difícil su desempeño, asimismo, los individuos con un exceso de masa grasa exhiben comúnmente un perfil lipídico alterado denominado dislipidemia aterogénica, estando ambos parámetros asociados al origen de enfermedades cardiovasculares (ECV). En éste estudio, se investiga la prevalencia de adiposidad corporal e irregularidades lipídicas y la relación entre estos como factores de riesgo asociados con las ECV en un grupo de funcionarios de la Policía Nacional Bolivariana.Material y Métodos: La muestra la conformaron 45 hombres y 20 mujeres. Se calculó el Índice de Masa Corporal (IMC) y el porcentaje de grasa (%GC) como indicadores adiposos y el perfil lipídico como indicadores bioquímicos. Resultados: Ambos grupos se ubicaron en categorías superiores a las recomendadas, obteniendo los hombres y mujeres un IMC y un %GC de 29,7 y 27,6% y 25,6 y 33,4%, ambos con diferencias significativas. De la misma forma, las mujeres superaron el límite superior de triglicéridos con 151,8 mg/dL, y estando cerca del límite superior del colesterol y a su vez, obtuvieron valores inferiores del HDL-c (40,5 mg/dL). Un porcentaje importante de ambos grupos presentó alteración en los indicadores bioquímicos a excepción del c-LDL. El colesterol y los triglicéridos tuvieron moderada correlación con el IMC en las mujeres, y moderadas, bajas y correlaciones inversas entre los parámetros de perfil lipídico con el IMC y el %GC en los hombres. Conclusiones: Se concluye que un importante porcentaje de funcionarios, muestran alta adiposidad, y ésta, relacionada con alteraciones en los lípidos, puede incrementar el riesgo para el desarrollo de ECV.
Pico de crecimiento y masa muscular en jugadores jóvenes de fútbol.
Authors: Hernández Camacho Juan Diego , Huelva Leal Ana Belen , Martínez-Sanz José Miguel , Lahoz Ruano María Dolores , Vázquez Carrión Javier
Published: December 2018

Introducción: Se ha observado que el pico de crecimiento podría ser un período importante en el desarrollo de deportistas. Objetivo: Determinar el pico de crecimiento y el estado madurativo en jugadores jóvenes de fútbol y analizar medidas antropométricas relacionadas con el desarrollo corporal.Material y métodos. Cincuenta y ocho jugadores se clasificaron en 3 categorías de edad diferentes, Sub-19 (post-pico de crecimiento), Sub-16 (en el pico) y Sub-14 (pre-pico). Se midieron la altura, la altura sentado, el peso, perímetros y pliegues para determinar el pico de crecimiento, el estado madurativo y los componentes corporales. Métodos estadísticos descriptivos (Desviación Estándar), análisis Anova de una vía y correlaciones de Pearson fueron calculados. El nivel de significación se fijó en p<0,05.  Resultados. La altura y el peso medios fueron de 167,63 (10,52) cm y 60,12 (12,43) kg, la grasa corporal media fue 13,49 (3,65)%. La masa muscular transversal del brazo, muslo y pantorrilla fueron 41,70 (10,82) cm2, 154,87 (38,02) cm2 and 85,76 (17,67) cm2. El pico de crecimiento y el estado madurativo medios fueron 13,97 (0,53) y 1,00 (1,92) años. Se encontraron diferencias significativas entre las categorías analizadas para los parámetros antropométricos y el estado madurativo. Se obtuvieron correlaciones entre el estado madurativo y los componentes antropométricos. Conclusiones. Se reportaron incrementos en componentes antropométricos y en las áreas musculares tras el pico de crecimiento. Consecuentemente, el pico de crecimiento y el estado madurativos deberían ser tenidos en cuenta en el manejo de deportistas jóvenes.
Integración de las normas locales y supranacionales de control del ejercicio de la abogacía
Authors: Goldfarb Mauricio
Published: December 2018

La responsabilidad disciplinaria es una de las consecuencias posibles del obrar irregular de los abogados, y se hace efectiva cuando se afecta el correcto ejercicio profesional.  La múltiple regulación de la conducta profesional (Ley de Colegiación, Código de Ética del Colegio, Código para la Abogacía del Mercosur y Código de la UIBA) provoca incertidumbres en los operadores jurídicos. Las normas supranacionales tienen aplicación supletoria, deben ser interpretadas en forma armónica y sistemática por el Tribunal de Disciplina en cada caso concreto y no derogan las normas locales en la materia.
O Tribunal de Contas e a “carona” no sistema de registro de preços: possibilidade de adesão tardia por empresas estatais em propostas vantajosas
Authors: Blanchet Luiz Alberto , Freitas Daniel Castanha de
Published: December 2018

As empresas estatais possuem inequívoca importância para a promoção do desenvolvimento. Detentoras de regime jurídico de direito privado com derrogações de direito público, suas contratações estão sujeitas à realização de licitação. Após a implementação do Sistema de Registro de Preços – SRP, previsto no art. 15, II, da Lei nº 8.666/93, surgiu a figura da adesão tardia, comumente denominada “carona”. Prevista inicialmente pelo Decreto nº 3.931/2001, tal instituto rapidamente ganhou notoriedade, atraindo a atenção do Tribunal de Contas da União, o qual, por meio dos acórdãos nos 1.487/2007 e 1.233/2012, restringiu a utilização da carona. Em razão das várias condicionantes impostas pela Corte de Contas, a adesão tardia caiu em desuso, até a edição do Decreto nº 7.892/2013. Com o novo regramento, disposições antes consideradas obscuras pelo TCU foram reformuladas, legitimando a utilização da carona em atas de registro gerenciadas por outros órgãos públicos. Nesse contexto, a pesquisa objetiva identificar os fundamentos – principiológicos e legais – para que as estatais possam aderir tardiamente às atas de registros de preços de forma segura, obtendo-se a proposta mais vantajosa para o órgão interessado, desde que preenchidos determinados requisitos tanto pelo aderente, quanto pelo que gerencia a ata de registro de preços.
El contrato administrativo a futuro
Authors: Alianak Raquel Cynthia
Published: December 2018

El trabajo ensaya una serie de principios que pueden ser elaborados como propios y específicos de la buena administración en la contratación pública, que complementan los formulados como inherentes a la “buena administración” en general así como los señalados como propios de la contratación pública global. Estos principios, que constituyen a la vez desafíos para el contrato administrativo en el futuro,  provienen de focalizar diversos tópicos o cuestiones que pueden darse en la etapa de selección así como en la de ejecución contractual, los cuales deben ser repensados en su contexto, diseño y modalidades de ocurrencia, a fin de que prevalezca el consenso activo, una mayor previsibilidad y predictibilidad para ambas partes, y criterios objetivos de interpretación; y todo ello tendiente a preservar la vida del contrato, evitar futuros conflictos que pueden eludirse, y satisfacer los intereses públicos y las necesidades involucradas en el objeto de la contratación.
Los contratos administrativos como herramienta de inclusión social
Authors: Antik Analía
Published: December 2018

A partir de los datos estadísticos oficiales en materia de pobreza e indigencia, de acceso al trabajo de personas con discapacidad y discriminación por cuestiones de género, así como los que surgen de informes de expertos y de organizaciones sociales, entendemos vital trabajar el instituto del contrato público, no solamente desde el punto de vista del respeto a las garantías de los particulares o como instrumento de mera satisfacción de las necesidades públicas, sino como una herramienta esencial de uso habitual en la planificación estratégica trazada dentro del marco de una política pública tendiente a la superación de los problemas de exclusión social señalados precedentemente.
La politica pubblica per la valutazione di qualità come il modo della valutazione dell'educazione come un bene pubblico
Authors: Godoy Dotta Alexandre
Published: December 2018

Il presente articolo ha come oggetto l’analisi storica e politica relativa alla nascita del concetto d’insegnamento di qualità nell’organizzazione dell’educazione in Brasile. Per raggiungere questo obiettivo, si concentra sullo status di“diritto all’educazione” o “servizio pubblico educazionale” come bene pubblico. Discorre sull’organizzazione del sistema educazionale brasiliano e presenta considerazioni sul problema delle interpretazioni restrittive e formali del sistema educativo, soprattutt   o nei termini della proposta neoliberale tipica degli anni novanta. Sviluppa riflessioni sul concetto di qualità e sulla possibilità di metterlo in pratica, attraverso le determinazioni della Costituzione brasiliana, secondo un’interpretazione sostanziale delle politiche pubbliche valutative.
Severe lung involvement in a female patient with undiagnosed systemic lupus erythematosus
Authors: Gavva T. N. , Kuzmenkova L. V. , Zemskov E. V. , Klyukvina N. G.
Published: December 2018

Systemic lupus erythematosus (SLE) is one of the most complex rheumatic diseases, which is due to the variety of its clinical forms and manifestations. The article describes a case of severe lung injury (LI) in a female patient with previously undiagnosed and untreated SLE, which led to a fatal outcome. A 33-year-old female patient diagnosed with pneumonia was delivered by an ambulance team to City Clinical Hospital Thirteen. Her leading complaints were dyspnea, joint pain, fever, and abdominal pain. During examination of the patient and analysis of her medical documents, attention was focused on a long history of articular syndrome, recurrent pneumonia with progressive pulmonary hypertension, general trophic, hematological changes, and the presence of autoantibodies, which was in aggregate the basis for the diagnosis of SLE with LI. This clinical case demonstrates the importance of the timely diagnosis and treatment initiation of SLE with LI. The diagnosis of SLE requires special attention to the patient's history and clinical and laboratory parameters. Specific immunological studies were conducted in doubtful cases. Rheumatological alertness is necessary, especially in relation to young women.
Risk factors for irreversible organ damages in peri- and postmenopausal women with systemic lupus erythematosus
Authors: Shkireeva S. Yu. , Zotkin E. G. , Lesnyak O. M.
Published: December 2018

Objective: to identify irreversible organ damages and factors influencing their development and to compare existing musculoskeletal injuries with the activity of systemic lupus erythematosus (SLE).Patients and methods. The investigation enrolled 197 peri- and postmenopausal female outpatients (mean age, 50.94±9.1 years) with SLE, who were followed up at Clinical Rheumatology Hospital Twenty-Five, Saint Petersburg. The mean disease duration was 9.7±7.5 years. Cytostatic and glucocorticoid (GC) treatment regimens and dosages in the examinees were analyzed on the basis of their primary medical records. The investigators assessed the current disease activity by the SLE Disease Activity Index (SLEDAI-2K) and the Lupus Low Disease Activity State (LLDAS) and irreversible organ damages by the Systemic Lupus International Collaborating Clinics (SLICC) Damage Index (DI).Results and discussion. 93.4% of the patients continued to receive GS therapy at the time of inclusion in the study. The median GS maintenance dose was 12.5 mg/day. Almost half of the patients (n=86/43.7%) were in menopause (its mean duration was 12.8±7.1 years). Half of the examinees were noted to have remission (n=36/18.3%) or low SLE activity (n=92/46.7%) according to the SLEDAI-2K. Fifty-seven (28.9%) patients met all 5 LLDAS criteria; at the same time other 96 (48.7%) patients met 4 out of the 5 criteria, the 5th criterion being a high GC maintenance dose. High DI scores of ≥4 were found in two thirds (n=131/66.5%) of the examinees. Musculoskeletal system injuries ranked first among other disorders: osteoporosis (OP) and muscle weakness were detected in 76 (38.6%) and 69 (35.0%) patients, respectively. Regression analysis involving all statistically significant factors showed that the degree of damage was influenced by age (p=0.013215), total GS dose (p=0.000047), and previous therapy with cyclophosphamide (p=0.041505).Conclusion. The contribution of GS therapy to irreversible damage accrual is obvious in peri- and postmenopausal women with SLE. Timely dose adjustment or complete withdrawal of GS during remission in SLE is one of the key factors that substantially reduce the risk of progression of irreversible organ damages due to the prevention of OP and osteoporotic fractures in these patients.
Specific features of regulatory T cells in patients with systemic lupus erythematosus
Authors: Torgashina A. V. , Solovyev S. К.
Published: December 2018

Regulatory T cells (Tregs) is a CD4+ lymphocyte subpopulation that maintains autotolerance by suppressing the activity of autoreactive lymphocytes. There is a hypothesis that functional defects or a smaller number of Tregs underlie the pathogenesis of a number of autoimmune diseases. The paper considers the main features of the phenotype of Tregs. It discusses the number of Tregs in both peripheral blood and affected organs in systemic lupus erythematosus, as well as the time course of changes in the level and functional abilities of different subpopulations of Tregs during immunosuppressive therapy. In addition, the paper presents various approaches to using Treg lymphocytes in the therapy of autoimmune diseases.
Approaches to therapy for depressions in neurology: prospects for the use of agomelatine
Authors: Romanov D. V. , Volel B. A. , Petelin D. S.
Published: December 2018

This review provides information on prospects for using the antidepressant agomelatine in neurological practice. The drug has a unique receptor profile, being a melatonin receptor type 1 and 2 agonist and a serotonin receptor subtype 2C antagonist. Due to this and in addition to antidepressant action, the drug has a number of other effects, such as analgesic, anti-apathetic, anti-asthenic, procognitive, anxiolytic, and sleep-normalizing ones, which are of great importance in the treatment of neurological diseases. There are clinical and experimental data that prove the high efficiency and safety of agomelatin in the follow-up of patients with post-stroke depression, Parkinson’s disease, Alzheimer’s disease, Pick’s disease, Huntington’s disease, chronic fatigue syndrome, and pain syndromes.
Chronic cerebrovascular diseases: use of vinpocetine in neurological practice (Round Table proceedings)
Authors: Parfenov V. A. , Zhivolupov S. A. , Zakharov V. V. , Belova L. A. , Lagoda O. V. , Esin R. G. , Vakhnina N. V. , Samartsev I. N.
Published: December 2018

The paper presents the proceedings of the Round Table with the participation of leading neurologists, which is devoted to chronic cerebrovascular diseases. It is noted that chronic cerebral ischemia (CCI), or dyscirculatory encephalopathy (DEP), is one of the most common neurological diagnoses in our country. The pathogenesis, clinical presentations, diagnosis and treatment of CCI (DEP) and its matching with vascular cognitive impairment (CI), which is regarded in foreign literature as the main manifestation of chronic cerebrovascular disease (CVD) were considered. The authors analyze clinical trials evaluating the efficacy of vinpocetine (Cavinton) in chronic CVD, dizziness, CI, as well as the use of new vinpocetine formulations, such as Cavinton Comforte, in various neurological diseases, dysphagia in particular, in poststroke patients.
Research Institute of Internal and Preventive Medicine, Branch, Federal Research Center, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Authors: Titarenko A. V. , Shishkin S. V. , Shcherbakova L. V. , Verevkin E. G. , Holmes M. , Bobak M. , Malyutina S. K.
Published: December 2018

Age-related cognitive changes in the Russian population have not been sufficiently studied.

Objective: to assess the time course of changes in cognitive functions (CF) in two serial measurements 9 years apart in the urban population of middle, elder, and senile age and their relationship to education level.

Patients and methods. A random male and female sample of45-69-year-old Novosibirsk residents (n=9360; HAPIEE project) was surveyed. CF was tested by standard methods. Resurvey was conducted in a random subsample (n=1663) included in this analysis. The mean follow-up period was 8.8 years (SD 1.1).

Results and discussion. The test for semantic verbal fluency revealed its dynamic decline in all age groups, which was steeper in women than in men (p<0.001). Persons aged 70 years and older showed negative changes in memory, verbal fluency, and attention (p<0.001). Persons with primary (6%) and vocational (26%) education had steeper rates of decline in delayed recall (p=0.011; 0.038) than those with university education (35%).

Conclusion.It is established that a dynamic decrease in CF begins at the age of55—59years; it is steeper among those aged 70 years and older. An age-related decline in memory function is accelerated in persons with lower educational levels.
Antibiotic prophylaxis is not associated with reduced urinary tract infection-related complications after cystectomy and ileal conduit
Authors: Kirkpatrick Carson , Haynes Allan , Sharma Pranav
Published: December 2018

Objectives: Majority of complications after ileal conduit urinary diversion with cystectomy are related to urinary tract infections (UTIs). Controversy exists regarding use of prophylactic antibiotics after surgery. We determined if prophylactic antibiotic use during ureteral stent placement after ileal conduit urinary diversion decreased incidence of UTI-related complications.
Methods: We retrospectively identified 75 consecutive patients who underwent ileal conduit urinary diversion with cystectomy at our institution from 2010 to 2016. Patients were stratified based on presence or absence of a UTI-related complication in the 90-day postoperative period. Means were compared with independent t-test and proportions with chi-square analysis. Multivariate logistic regression was performed to determine independent predictors of UTI-related complications.
Results: Forty-five patients (60%) were prescribed prophylactic antibiotics after surgery. Mean duration of antibiotic use was 15 d, and mean duration of ureteral stenting was 25 d. Most common antibiotics used included fluoroquinolones (n = 23, 30.7%) followed by sulfamethoxazole-trimethoprim (n = 14, 18.7%). Rate of 90-day UTI-related complications was 36% (n = 27), and 90-day UTI-related readmission rate was 14.7% (n = 11). On bivariate and multivariate analysis, prophylactic antibiotic use was not associated with reduced 90-day UTI-related complications (P > 0.05). Patients prescribed prophylactic antibiotics had increased incidence of Clostridium difficile infections in the 90-day postoperative period compared to controls (20% vs. 3.3%; P = 0.038).
Conclusions: Prophylactic antibiotic use after ileal conduit urinary diversion with cystectomy was not associated with reduced UTI-related complications, and rate of Clostridium difficile infections was higher in this patient cohort. The effect of early removal of ureteral stents on UTI risk still has to be elucidated.
Method for selective quantification of immune and inflammatory cells in the cornea using flow cytometry
Authors: Ogawa Mamoru , Inomata Takenori , Shiang Tina , Tsubota Kazuo , Murakami Akira
Published: December 2018

The cornea serves as a protective surface against the environment (i.e., allergens, pollutants, desiccation and microorganisms) and promotes vision, made possible by corneal transparency. This protocol describes corneal preparation for flow cytometry to assess cells localized in the cornea. Our model details the process, from determining how many corneas are needed in the experiment to corneal excision to digestion and staining of the cornea cells. The simplicity of the model allows for systematic analysis of different corneal mechanisms of immunity, inflammation, angiogenesis and wound healing. In corneal transplantation, residential immune and inflammatory cells are key to the mechanisms that underlie angiogenesis, opacity, and graft rejection. In addition, this model can also elucidate cellular mechanisms mediating corneal graft outcomes and wound healing. Lastly, this model can be used to analyze the efficacy of new medications such as instillation and subconjunctival injections and assess the potential of therapeutic molecules to enhance graft survival and wound healing in vivo.
RNA isolation from Peyer's patch lymphocytes and mononuclear phagocytes to determine gene expression profiles using NanoString technology
Authors: Singh Navjot , C. Gallagher Heather , Song Renjie , K. Dhinsa Jaskiran , R. Ostroff Gary , De Jesus Magdia
Published: December 2018

Sampling and immune surveillance within gut-associated lymphoid tissues (GALT) such as the intestinal Peyer’s patch (PP) occurs by an elegantly orchestrated effort that involves the epithelial barrier, B and T lymphocytes, and an extensive network of mononuclear phagocytes. Although we now understand more about the dynamics of antigen and microbial sampling within PPs, the gene expression changes that occur in individual cell subsets during sampling are not well characterized. This protocol describes the isolation of high-quality RNA from sorted PP, B and T-lymphocytes, and CD11c+ phagocytes for use with nCounter-nanostring technology. This method allows investigators to study gene expression changes within PPs in response to antigens, microbes, and oral vaccine delivery vehicles of interest that are sampled.
Exosomes molecular diagnostics: Direct conversion of exosomes into the cDNA for gene amplification by two-step polymerase chain reaction
Authors: Haque Shabirul , R. Vaiselbuh Sarah
Published: December 2018

Exosomes are cell derived lipid nanoparticle with a size of 30–100 nm in diameter, found in almost all biological fluids. The composition of the exosomes is mainly lipid, proteins, RNA, DNA, and non-coding RNAs. Currently, most available methods and commercial kits for exosomal-RNA (Exo-RNA) isolation have limitations and shortcomings. Small starting volume of exosomes and the use of extraction/filtration columns results usually insufficient yield of exosomal RNA after isolation. The majority of RNA contained in purified exosomes range in size from 15–500 nucleotides. Some RNA isolation kits are well suited for small RNA transcripts isolation but larger mRNA transcripts are hard to detect. For all of the kits, the cost prize per sample analyzed is very high. Our current method provides a novel way for direct conversion of exosomes into cDNA synthesis (Exo-cDNA) and subsequent gene detection by polymerase chain reaction (PCR). This method has several advantages compared to established available kits. No extraction column is utilized in this procedure which means total recovery of exosomal RNA with maximal yield. In addition, this method is fast and uses a minimal amount of lab supplies, thereby reducing the overall working costs. Our findings suggest that direct conversion of exosomes into cDNA and subsequent gene amplification by two step PCR is a most efficient and reproducible technique. This novel method can be applied to and is useful to advance molecular research of exosomes by solving the problem of low molecular yields.
A Neuroscience-Oriented Research Approach to Borderline Personality Disorder
Authors: Stanley Barbara , Perez-Rodriguez M. Mercedes , Labouliere Christa , Roose Steven
Published: December 2018

Traditionally, the study of personality disorders had been based on psychoanalytic or behavioral models. Over the past two decades, there has been an emerging neuroscience model of borderline personality disorder (BPD) grounded in the concept of BPD as a condition in which dysfunctional neural circuits underlie its pathological dimensions, some of which include emotion dysregulation (broadly encompassing affective instability, negative affectivity, and hyperarousal), abnormal interpersonal functioning, and impulsive aggression. This article, initiated at a joint Columbia University- Cornell University Think Tank on BPD with representation from the Icahn School of Medicine at Mount Sinai, suggests how to advance research in BPD by studying the dimensions that underlie BPD in addition to studying the disorder as a unitary diagnostic entity. We suggest that linking the underlying neurobiological abnormalities to behavioral symptoms of the disorder can inform a research agenda to better understand BPD with its multiple presentations.
Neuronal Adenosine A2A Receptors Are Critical Mediators of Neurodegeneration Triggered by Convulsions
Authors: Canas Paula M. , Porciúncula Lisiane O. , Simões Ana Patrícia , Augusto Elisabete , Silva Henrique B. , Machado Nuno J. , Gonçalves Nélio , Alfaro Tiago M. , Gonçalves Francisco Q. , Araújo Inês M. , Real Joana I. , Coelho Joana E. , Andrade Geanne M. , Almeida Ramiro D. , Chen Jiang-Fan , Köfalvi Attila , Agostinho Paula , Cunha Rodrigo A.
Published: December 2018

Neurodegeneration is a process transversal to neuropsychiatric diseases and the understanding of its mechanisms should allow devising strategies to prevent this irreversible step in brain diseases. Neurodegeneration caused by seizures is a critical step in the aggravation of temporal lobe epilepsy, but its mechanisms remain undetermined. Convulsions trigger an elevation of extracellular adenosine and upregulate adenosine A2Areceptors (A2AR), which have been associated with the control of neurodegenerative diseases. Using the rat and mouse kainate model of temporal lobe epilepsy, we now tested whether A2AR control convulsions-induced hippocampal neurodegeneration. The pharmacological or genetic blockade of A2AR did not affect kainate-induced convulsions but dampened the subsequent neurotoxicity. This neurotoxicity began with a rapid A2AR upregulation within glutamatergic synapses (within 2 h), through local translation of synaptic A2AR mRNA. This bolstered A2AR-mediated facilitation of glutamate release and of long-term potentiation (LTP) in CA1 synapses (4 h), triggered a subsequent synaptotoxicity, heralded by decreased synaptic plasticity and loss of synaptic markers coupled to calpain activation (12 h), that predated overt neuronal loss (24 h). All modifications were prevented by the deletion of A2AR selectively in forebrain neurons. This shows that synaptic A2AR critically control synaptic excitotoxicity, which underlies the development of convulsions-induced neurodegeneration.
miR-505-3p is a repressor of puberty onset in female mice
Authors: Zhou Yuxun , Tong Li , Wang Maochun , Chang Xueying , Wang Sijia , Li Kai , Xiao Junhua
Published: December 2018

Puberty onset is a complex trait regulated by multiple genetic and environmental factors. In this study, we narrowed a puberty-related QTL region down to a 1.7 Mb region on chromosome X in female mice and inferred miR-505-3p as the functional gene. We conducted ectopic expression of miR-505-3p in the hypothalamus of prepubertal female mice through lentivirus-mediated orthotopic injection. The impact of miR-505-3p on female puberty was evaluated by the measurement of pubertal/reproduction events and histological analysis. The results showed that female mice with overexpression of miR-505-3p in the hypothalamus manifested later puberty onset timing both in vaginal opening and ovary maturation, followed by weaker fertility lying in the longer interval time between mating and delivery, higher abortion rate and smaller litter size. We also constructed miR-505-3p-knockout mice by CRISPR/Cas9 technology. miR-505-3p-knockout female mice showed earlier vaginal opening timing, higher serum gonadotrophin and higher expression of puberty-related gene in the hypothalamus than their WT littermates. Srsf1 proved to be the target gene of miR-505-3p that played the major role in this process. The results of RNA immunoprecipitation sequencing showed that SRSF1 (or SF2), the protein product of Srsf1 gene, mainly bound to ribosome protein (RP) mRNAs in GT1-7 cells. The collective evidence implied that miR-505-3p/SRSF1/RP could play a role in the sexual maturation regulation of mammals.
Scrub typhus infection, not a benign disease: an experience from a tertiary care center in Northern India
Authors: Jain Deepak , Nand Nitya , Giri Kajaree , Bhutani Jaikrit
Published: December 2018

Introduction. Scrub typhus is an acute febrile illness caused by Orientia tsutsugamushi. It is characterised by fever, rash, myalgia and diffuse lymphadenopathy. Most common complications are sepsis, shock, acute respiratory distress syndrome (ARDS), hepatitis, pre renal azotemia and multi organ dysfunction syndrome (MODS). Mortality rates range from 7-30% in untreated cases. Scrub typhus is endemic to a part of the world known as ‘the tsutsugamushi triangle’. River banks, grassy areas generally harbour scrub typhus infection; however, recently there has been an increase in prevalence of infection from dry regions like Haryana.
Objective. To assess the clinical spectrum and complications of scrub typhus infection at a tertiary care centre in North India.
Material and methods. An observational study was conducted on patients >14 years old with acute febrile illness >7 days duration, admitted indoor from emergency department, from July to November 2017. Suspected cases were tested for specific IgM antibodies against Orientia tsutsugamushi by ELISA.
Results. Among the 230 patients, screened for scrub typhus infection, 39 (16.95%) came out to be positive. Most common patient complaints were fever followed by cough and breathlessness, myalgia, nausea, vomiting and behavioral abnormality. 15% of patients required inotropic support initially and 48% had oxygen saturation of less than 90% at the time of presentation. Pleural effusion and crepitations were present in 41% of patients. Most common biochemical alterations were: abnormal liver function tests (95%), followed by thrombocytopenia, anemia, abnormal renal function tests, and hyponatremia. 12 patients (30.7%) were shifted to intensive care unit, 8 of which (20.5%) needed invasive mechanical ventilation and 4 patients (10.3%) underwent hemodialysis. Various complications were noted in 89.7% of cases, the most common being ARDS followed by sepsis, acute kidney injury (AKI) and meningitis. The mortality rate in this study was 18%.
Conclusions. This study emphasizes that scrub typhus infection is on a rampant resurgence and it is associated with significant complications. High degree of suspicion as well as development of effective measures to treat, control and prevent is critical to lower the disease burden.
Increased levels of Angiogenic Factors In Microvascular Angina
Authors: Roshani-Asl Elmira , Rasmi Yousef , Khadem-Ansari Mohammad-Hasan , Seyed-Mohammadzad MirHossein , Rostamzadeh Alireza , Ghaffari Fereshteh , Mokarizadeh Narmin
Published: December 2018

Background. Recent studies have suggested that angiogenic factors may affect vascular endothelial integrity. On the other hand, endothelial dysfunction is the main pathological mechanism in microvascular angina (MVA) or cardiac syndrome X. Therefore, we aimed to determine the levels of angiogenic factors in MVA patients. In addition, we investigated the effects of metoprolol, as a beta blocker agent, on the serum levels of these factors.Methods. Thirty patients with MVA (17 female/13 male; mean age: 55.53±9.18 years) and twenty healthy controls (14 female/6 male; mean age: 51.40±9.16 years) were enrolled.The serum amounts of angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) and tyrosine kinase-2 receptor (Tie-2) were measured in healthy controls, MVA patients at baseline and after metoprolol therapy (25 mg for one month) by enzyme-linked immunosorbent assay.Results. The levels of Ang-2 and Tie-2 were significantly higher in MVA patients at baseline in comparison with controls (Ang-2: 277.02±186.08 vs.164.46±49.83 ng/l, P=0.011; Tie-2: 28.97±18.85 vs. 14.90±4.05 ng/ml, P=0.002; respectively). But this difference in the Ang-1 levels was not significant (P=0.829). Additionally, the levels of angiogenic factors in MVA patients after metoprolol therapy were not significantly changed in comparison with the baseline status (P>0.05).Conclusion. Our results considered a possible role for angiogenic factors in the pathophysiology of MVA, which need ‎further investigation for elucidation. In addition, this study has not showed an effective role for metoprolol in changing the angiogenic factors levels as a therapeutic agent in MVA.
Penggunaan Tools Assesment Biopsikososial dan Spiritual Anak yang menjadi Korban Perceraian Orang Tua
Authors: - Mayangsari
Published: December 2018

Abstract. Divorce often produces both positive and negative influences in all aspects of children’s life. This research intends to seek whether parents’ divorce can affect a child’s health condition, psychology and spiritual which are referred as a bio-psychosocial and spiritual tools. By utilizing these tools, it is expected that a better understanding can be drawn to what extent divorce may affect children of divorced parents. This thesis uses a qualitative approach with a case study research methodology where data collection techniques includes interviews, observation and documentation. The findings indicate that biopsychosocial and spiritual tools can provides information on the impact upon children with divorced parents. These impacts can be comprehended through the biological aspects, how health conditions of children altered prior to and following the separation of parents. The psychological and spiritual conditions can be perceived through how a child interacts with the environment. Abstrak. Perceraian kerap kali membawa pengaruh dalam segala aspek kehidupan anak baik pengaruh yang bersifat positif maupun negatif. Penelitian ini perlu dilakukan untuk mengetahui apakah perceraian orang tua dapat mempengaruhi kondisi kesehatan anak, psikologis anak serta spiritual anak atau dapat disebut sebagai biopsikososial dan spiritual. Dengan memperhatikan aspek tersebut diharapkan perceraian tidak menjadi momok yang menakutkan bagi anak karena meskipun orang tua mereka berpisah, mereka masih dapat merasakan kasih sayang seutuhnya dari kedua orang tua. Pendekatan yang peneliti gunakan dalam skripsi ini adalah metodologi penelitian kualitatif dengan penelitian studi kasus, dimana teknik pengumpulan data penulis dilakukan melalui wawancara, observasi dan dokumentasi. Adapun hasil temuan yang peneliti dapatkan mengenai dampak biopsikososial dan spiritual korban perceraian orang tuanya, adalah dapat dilihat melalui aspek biologis yaitu bagaimana kondisi kesehatan anak sebelum dan sesudah perceraian orang tuanya, aspek psikologis yaitu bagaimana konsep diri seorang anak dengan perceraian orang tuanya, aspek sosial dan spiritual adalah bagaimana interaksi seorang anak dengan lingkungan, apakah perceraian benar-benar memberikan pengaruh terhadap aspek-aspek tersebut diatas.
The Involvement of Parents in the Children’s Care Process; a Comparison of Various Countries
Authors: Budiarti Santoso Meilanny
Published: December 2018

Abstract. To be a parent means to put the commitment of time and unselfish self during serving as a parent as well as spouse, part of big family, friends, and colleagues. Someone is becoming a parent because adults have been prepared to respond positively to the existing of a baby as well as strong community drive as the influence to have children. This article is aiming at describing the parental involvement in child care influenced by culture and geographical location of their living. This article is formulated using literature study on child care practices around the world. Parent is defined as individuals who provide care, protection, and guidance since the baby until growing up. During conducting their role in providing care, guidance and needs fulfillment, the parent is very much influenced by culture and geographical location. Therefore the role and parental involvement in child care practice are unique in every region/country.Abstrak. Menjadi orang tua berarti menempatkan komitmen waktu dan tak mementingkan diri sendiri selama berperan sebagai orang tua dan suami/istri, bagian dari keluarga besar, teman dan kolega. Seseorang yang sedang menjadi orang tua dikarenakan masa dewasa yakni sudah disediakan untuk merespon secara positif kehadiran bayi. Juga dukungan dan pengaruh kuat dari masyarakat agar memiliki anak. Artikel ini bertujuan menggambarkan keterlibatan orang tua dalam mengasuh anak yang dipengaruhi oleh kebudayaan dan lokasi geografis tempat tinggal. Artikel ini disusun menggunakan studi pustaka tentang praktik pengasuhan anak di dunia. Orang tua diartikan sebagai individu yang memberikan kepedulian, perlindungan dan arahan sejak bayi hingga tumbuh dewasa. Selama berperan memberi perawatan, arahan dan memenuhi kebutuhan anak, orang tua sangat dipengaruhi oleh budaya dan lokasi geografis. Maka dari itu, peran dan keterlibatan orang tua dalam praktik mengasuh anak bersifat unik di setiap daerah/negara.
Left Atrial Appendage Closure Devices for Stroke Prevention in Patients with Non-Valvular AF
Authors: McBride Daniel A ,   Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA, Markman Timothy M ,   Division of Cardiology, Electrophysiology Section, Hospital of the University of Pennsylvania, Philadelphia, PA, Liang Jackson J ,   Division of Cardiology, Electrophysiology Section, Hospital of the University of Pennsylvania, Philadelphia, PA, Santangeli Pasquale ,   Division of Cardiology, Electrophysiology Section, Hospital of the University of Pennsylvania, Philadelphia, PA
Published: December 2018

The left atrial appendage (LAA) may be involved in offloading atrial pressure during left ventricular systole. As ventricular rate increases, LAA emptying decreases during early diastole causing increased risk of thrombus formation particularly in patients with non-valvular AF (NVAF). The LAA is the site of thrombus formation in more than 90 % of patients with NVAF, so is an important target for thromboembolic prophylaxis in these patients. Anticoagulation therapy is used to treat NVAF, but it has long-term complications and may be contraindicated in some patients. Therefore, alternative strategies to reduce embolic risk in patients with AF have been developed. These include percutaneous, thoracoscopic, and open closure strategies. This study reviews the safety and efficacy of these strategies, comparing these approaches and devices with pharmacological strategies. There is little data to endorse one strategy over another. Given the minimal evidence available, recommendations in support of LAA occlusion remain weak and guidelines have called for more research and coding of endpoints for this emerging technology.
Management of Cardiovascular Disease During Pregnancy
Authors: Scott Nandita S ,   Division of Cardiology, Massachusetts General Hospital, and Harvard Medical School, Boston, MA
Published: December 2018

Cardiovascular disease is a leading cause of maternal death. The normal cardiovascular hemodynamic adaptations to pregnancy are remarkable, but tolerated without difficulty in the majority of women. However, in women with cardiovascular dysfunction, these adaptations may precipitate cardiovascular decompensation. Risk stratification of pregnancy risk should preferably take place before conception. Management of these women requires multidisciplinary involvement of all key areas, including cardiology, nursing, maternal/fetal medicine and obstetric anesthesia. For higher-risk lesions, pregnancy should be managed in centers with expertise in this field.
Effectiveness of antiperching devices against the Feral Pigeons
Authors: Matsyura A. V.
Published: December 2018

Nowadays, the problem of pest-control at grain processing plants is of great and urgent importance. The feral pigeons recently became the most abundant species that use the territory of such enterprises all year round. We undertook the investigation towards effectiveness of pigeons repelling during the course of pest management. We use anti-roosting spikes, optical and repellent gel along with the wire systems to protect the industrial area against the pigeons. Our study contributed to the fact, that the mechanical antiperching devices still the most effective as for their use in pigeon control and management.
Hawk kite as potential bird scare device (the case of pigeons and grain processing factory)
Authors: Matsyura A. V.
Published: December 2018

Here we presented the preliminary results of hawk kite usage against the feral pigeons in some grain processing factory. We studied the temporal and spatial patterns of repellent effect and bird behavior. We suggested the feral pigeons gradually increase the level of tolerance towards the hawk kite if no additional repellent measures were undertaken. Moreover, even initially the feral pigeons demonstrate higher tolerance towards the hawk kite compared to the Rooks or Hooded Crows.
Antagonistic activity of Bacillus subtilis strains isolated from various sources
Authors: Irkitova A. N. , Grebenshchikova A. V. , Matsyura A. V.
Published: December 2018

An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is Bacillus subtilis. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of B. subtilis, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of B. subtilis. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (Escherichia coli) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of B. subtilis have antimicrobial activity against a wild strain of E. coli, but to varying degrees. We identified strains of B. subtilis with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.
Morphological diversity of Bacillus subtilis
Authors: Irkitova A. N. , Grebenshchikova A. V. , Yatsenko E. S. , Speranskaya N. Y. , Matsyura A. V.
Published: December 2018

Bacillus subtilis is the most studied bacterium from genus Bacillus and this microorganism is widespread in nature. B. subtilis is often found in fresh and seawater, in the rhizosphere, in the soil (especially contaminated with feces and manure). The large variety of strains are available in collections of microorganisms, but strains isolated from the rhizosphere of various plants are particularly interesting for science and practical use. At the same time, the morphological criterion (the form and other features of the colonies) is the main one in the identification of B. subtilis at the first stages of the study and allows to refuse of extraneous strains before use more expensive methods and materials. The article presents the description of the morphological diversity of B. subtilis strains that are used in agriculture. Typical R–colonies are characteristic of bacteria B. subtilis, and we established 2 morphologically diverse forms of these colonies in the studied strains.

 
Project and Operations Management of Machine-Building Production
Authors: Tsyrkov Alexander V. , Kuznetsov Pavel M. , Tsyrkov George A. , Yermokhin Yegor A. , Moskvin Valery K.
Published: December 2018

Introduction. The aim of this work is to develop the main guidelines of project and operations management at machine-building enterprises.
Materials and Methods. The authors reviewed the state of application of the project and operations management of machine-building production in the automated mode. The review showed the complexity of its application due to a large number of factors, which must be taken into account when implementing. An approach was developed that allows solving the task using the automation of analysis processes and decision-making in production management.
Results. The article establishes the main guidelines of the project and operations management, aimed at increasing productivity and reducing production costs based on modeling the state of production environment. It also defines the requirements for the model. A prognostic time model for forecasting the state of the enterprise production system has been developed.
Conclusions.The tasks have been solved in this article allow increasing the level of automation of the processes of project and operations management of the enterprise in the conditions of quick-change production. The implementation of the developed approach to project and operations management of the enterprise will allow streamlining the launch of products with a reduction in the amount of work in progress and increasing the productivity of output.

Keywords: technological process, project and operations management, design, prognostic model, production, labor intensity, productivity

For citation: Tsyrkov A. V., Kuznetsov P. M., Tsyrkov G. A., Yermokhin Ye. A., Moskvin V. K. Project and Operations Management of Machine-Building Production. Vestnik Mordovskogo universiteta = Mordovia University Bulletin. 2018;
28(4):511–522. DOI: https://doi.org/10.15507/0236-2910.028.201804.511-522