bluuebunny/crossref_metadata_2025 huggingface dataset:
Compression Properties and Its Prediction of Wood-Based Sandwich Panels with a Novel Taiji Honeycomb Core
Authors: Hao Jingxin , Wu Xinfeng , Oporto-Velasquez Gloria , Wang Jingxin , Dahle Gregory
Published: August 2020

The transverse compression property is one of most important aspects of the mechanical performance of a sandwich structure with a soft core. An experiment, analytical method and three digital strain measurement systems were applied to investigate the compression behavior and the failure mechanism for a wood-based sandwich structure with a novel Taiji honeycomb core. The results show that the structure of the Taiji honeycomb can improve dramatically on compression strength and modulus of composite compared to that of a traditional hexagonal one. There was no obvious deflection in the transverse direction detected by the three digital images before the buckling of the honeycomb occurred. An analytical equation between the key structure parameters and properties of the composite were applied to predict its threshold stresses and modulus. The properties of the core determine the strength of the entire structure, but the compression strength decreases slightly with an elevated core thickness, and its effect on the compression modulus can be neglected. Both the surface sheets and loading speed have little impact on the compression strength and modulus, respectively.
Long-Term Effects of Fuels Treatments, Overstory Structure, and Wildfire on Tree Regeneration in Dry Forests of Central Washington
Authors: Rossman Allison K. , Bakker Jonathan D. , Peterson David W. , Halpern Charles B.
Published: August 2020

The long-term effectiveness of dry-forest fuels treatments (restoration thinning and prescribed burning) depends, in part, on the pace at which trees regenerate and recruit into the overstory. Knowledge of the factors that shape post-treatment regeneration and growth is limited by the short timeframes and simple disturbance histories of past research. Here, we present results of a 15-year fuels-reduction experiment in central Washington, including responses to planned and unplanned disturbances. We explore the changing patterns of Douglas-fir regeneration in 72 permanent plots (0.1 ha) varying in overstory abundance (a function of density and basal area) and disturbance history—the latter including thinning, prescribed burning, and/or wildfire. Plots were measured before treatment (2000/2001), soon afterwards (2004/2005), and more than a decade later (2015). Thinning combined with burning enhanced sapling recruitment (ingrowth) into the overstory, although rates of ingrowth were consistently low and greatly exceeded by mortality. Relationships between seedling frequency (proportion of quadrats within a plot) and overstory abundance shifted from weakly negative before treatment to positive after thinning, to neutral in the longer term. However, these relationships were overshadowed by more recent, higher-severity prescribed fire and wildfire that stimulated seedling establishment while killing advanced regeneration and overstory trees. Our results highlight the dependence of regeneration responses on the history of, and time since, fuels treatment and subsequent disturbance. Managers must be aware of this spatial and temporal complexity and plan for future disturbances that are inevitable but unpredictable in timing and severity.
Survival of Quercus alba (White Oak) Advance Reproduction in Small Group and Single Tree Openings
Authors: Spetich Martin A.
Published: August 2020

Survival probabilities of white oak (Quercus alba) in small circular group and single tree openings ranging in size from 0.001 to 0.175 ha twelve years after opening creation are presented. At the beginning of the study, 3948 advance reproduction white oak trees were measured and tagged to determine survival of each tagged seedling at the end of the study. Logistic regression indicated that variables important in predicting advance reproduction survival included initial seedling basal diameter, aspect, slope, canopy opening size, opening border tree height and treatment for control of understory competition. Survival probability ranged from 10% to 90% depending on the combination of and disposition of variables. For these small openings, the greatest probability of survival of advance reproduction resulted when advance reproduction initial basal diameters were ≥1 cm, when the height of trees bordering the openings were relatively short, with understory chemical competition control, in the largest canopy openings, on 6% slopes, and on southwest and northwest aspects. These criteria can help managers select sites and treatment options for group opening creation that provide optimal conditions for advance reproduction survival.
Methodology for the Determination of Fruit, Vegetable, Nut and Legume Points for Food Supplies without Quantitative Ingredient Declarations and Its Application to a Large Canadian Packaged Food and Beverage Database
Authors: Vergeer Laura , Ahmed Mavra , Franco-Arellano Beatriz , Mulligan Christine , Dickinson Kacie , Bernstein Jodi T. , Labonté Marie-Ève , L’Abbé Mary R.
Published: August 2020

Nutrient profiling (NP) models are useful tools for objectively and transparently quantifying the nutritional quality of packaged foods and beverages. Many NP models incorporate ingredients beneficial for health (e.g., fruits, vegetables, nuts, legumes (FVNL)) in addition to less healthful nutrients or components, assigning points based on the proportion of the product that contains FVNL ingredients. However, with food labelling in most countries lacking mandatory quantitative ingredient declarations (QUIDs), there is potential for the estimation of FVNL points to be ambiguous and inconsistent. The purpose of this article was to describe the development and application of methodology for estimating FVNL points for products without QUIDs, based on the position of FVNL components within the ingredients list. Using this method, FVNL points were calculated for packaged foods and beverages in the University of Toronto Food Label Information Program 2017 database (n = 17,337). Distributions of FVNL points were examined overall and by food category. This study provides evidence of the feasibility of this method in distinguishing between products with differing amounts of FVNL ingredients. This method will be valuable for researchers and policymakers in ensuring consistent, objective and reproducible estimations of FVNL points—and consequently, assessments of product healthfulness—for food supplies without QUIDs.
Publisher’s Note: Continued Publication of Gastroenterology Insights by MDPI
Authors: Vazquez Franck
Published: August 2020

Gastroenterology Insights was launched in 2009 and it has been published over the past eleven years by PAGEPress Publications, with a total of 88 papers released [...]
Distinct Alteration of Gene Expression Programs in the Small Intestine of Male and Female Mice in Response to Ablation of Intestinal Fabp Genes
Authors: Chen Yiheng , Agellon Luis B.
Published: August 2020

Fatty acid-binding proteins (Fabps) make up a family of widely distributed cytoplasmic lipid-binding proteins. The small intestine contains three predominant Fabp species, Fabp1, Fabp2, and Fabp6. Our previous studies showed that Fabp2 and Fabp6 gene-disrupted mice exhibited sexually dimorphic phenotypes. In this study, we carried out a systematic comparative analysis of the small intestinal transcriptomes of 10 week-old wild-type (WT) and Fabp gene-disrupted male and female mice. We found that the small intestinal transcriptome of male and female mice showed key differences in the gene expression profiles that affect major biological processes. The deletion of specific Fabp genes induced unique and sex-specific changes in the gene expression program, although some differentially expressed genes in certain genotypes were common to both sexes. Functional annotation and interaction network analyses revealed that the number and type of affected pathways, as well as the sets of interacting nodes in each of the Fabp genotypes, are partitioned by sex. To our knowledge, this is the first time that sex differences were identified and categorized at the transcriptome level in mice lacking different intestinal Fabps. The distinctive transcriptome profiles of WT male and female small intestine may predetermine the nature of transcriptional reprogramming that manifests as sexually dimorphic responses to the ablation of intestinal Fabp genes.
Sexual Difference in the Optimum Environmental Conditions for Growth and Maturation of the Brown Alga Undaria pinnatifida in the Gametophyte Stage
Authors: Sato Yoichi , Endo Hikaru , Oikawa Hiroki , Kanematsu Koichi , Naka Hiroyuki , Mogamiya Miho , Kawano Shigeyuki , Kazama Yusuke
Published: August 2020

Undaria pinnatifida is an annual brown kelp growing naturally in coastal areas as a major primary producer in temperate regions and is cultivated on an industrial scale. Kelps have a heteromorphic life cycle characterized by a macroscopic sporophyte and microscopic sexual gametophytes. The sex-dependent effects of different environmental factors on the growth and maturation characteristics of the gametophyte stage were investigated using response surface methodology. Gametophytes were taken from three sites in Japan: Iwate Prefecture, Tokushima Prefecture, and Kagoshima Prefecture in order to confirm the sexual differences in three independent lines. Optimum temperature and light intensity were higher for males (20.7–20.9 °C and 28.6–33.7 µmol m−2 s−1, respectively) than females (16.5–19.8 °C and 26.9–32.5 µmol m−2 s−1), and maturity progressed more quickly in males than females. Optimum wavelengths of light for growth and maturation of the gametophytes were observed for both blue (400–500 nm, λmax 453 nm) and green (500–600 nm; λmax 525 nm) lights and were sex-independent. These characteristics were consistent among the three regional lines. Slower growth optima and progress of maturation could be important for female gametophytes to restrict fertilization and sporophyte germination to the lower water temperatures of autumn and winter, and suggest that the female gametophyte may be more sensitive to temperature than the male. The sexual differences in sensitivity to environmental factors improved the synchronicity of sporeling production.
Variations in Canopy Cover and Its Relationship with Canopy Water and Temperature in the Miombo Woodland Based on Satellite Data
Authors: Zimba Henry , Coenders-Gerrits Miriam , Kawawa Banda , Savenije Hubert , Nyambe Imasiku , Winsemius Hessel
Published: August 2020

Understanding the canopy cover relationship with canopy water content and canopy temperature in the Miombo ecosystem is important for studying the consequences of climate change. To better understand these relationships, we studied the satellite data-based land surface temperature (LST) as proxy for canopy temperature, leaf area index (LAI), and the normalized difference vegetation index (NDVI) as proxies for canopy cover. Meanwhile, the normalized difference infrared index (NDII) was used as a proxy for canopy water content. We used several statistical approaches including the correlated component regression linear model (CCR.LM) to understand the relationships. Our results showed that the most determinant factor of variations in the canopy cover was the interaction between canopy water content (i.e., NDII) and canopy temperature (i.e., LST) with coefficients of determination (R2) ranging between 0.67 and 0.96. However, the coefficients of estimates showed the canopy water content (i.e., NDII) to have had the largest percentage of the interactive effect on the variations in canopy cover regardless of the proxy used i.e., LAI or NDVI. From 2009–2018, the NDII (proxy for canopy water content) showed no significant (at alpha level 0.05) trend. However, there was a significant upward trend in LST (proxy for canopy temperature) with a magnitude of 0.17 °C/year. Yet, the upward trend in LST did not result in significant (at alpha level 0.05) downward changes in canopy cover (i.e., proxied by LAI and NDVI). This result augments the observed least determinant factor characterization of temperature (i.e., LST) on the variations in canopy cover as compared to the vegetation water content (i.e., NDII).
Coaching Through Technology: A Systematic Review into Efficacy and Effectiveness for the Ageing Population
Authors: Bevilacqua Roberta , Casaccia Sara , Cortellessa Gabriella , Astell Arlene , Lattanzio Fabrizia , Corsonello Andrea , D’Ascoli Paola , Paolini Susy , Di Rosa Mirko , Rossi Lorena , Maranesi Elvira
Published: August 2020

Background: Despite the evidence on the positive role of self-management, the adoption of health coaching strategies for older people is still limited. To address these gaps, recent efforts have been made in the ICT sector in order to develop systems for delivering coaching and overcoming barriers relating to scarcity of resources. The aim of this review is to examine the efficacy of personal health coaching systems for older adults using digital virtual agents. Methods: A systematic review of the literature was conducted in December 2019 analyzing manuscripts from four databases over the last 10 years. Nine papers were included. Results: Despite the low number of studies, there was evidence that technology-integrated interventions can deliver benefits for health over usual care. However, the review raises important questions about how to maintain benefits and permanence of behavior change produced by short-term interventions. Conclusion: These systems offer a potential tool to reduce costs, minimize therapist burden and training, and expand the range of clients who can benefit from them. It is desirable that in the future the number of studies will grow, considering other aspects such as the role of the virtual coaches’ characteristics, social-presence, empathy, usability, and health literacy.
Counteracting Abuse in Health Care: Evaluating a One-Year Drama Intervention with Staff in Sweden
Authors: Zbikowski Anke , Brüggemann A. Jelmer , Wijma Barbro , Swahnberg Katarina
Published: August 2020

In Northern European countries 13–28% of female patients seeking gynecological health care have reported abuse by health care staff (AHC). We conducted workshops with health care staff using the improvised role-play method Forum Play (FP), based on techniques developed by Boal. The study explores to what extent the intervention increased the staff’s awareness of AHC and their ability to take action against it. A total of 16 half-day FP workshops were conducted with staff from a Swedish women’s clinic over one year. Self-reported questionnaires were distributed to all staff before, during, and after the intervention. Primary outcome measures were the number of reported occasions of AHC and FP participants’ ability to act in AHC-situations. We found an increase in the participants’ self-reported ability to act in AHC-related situations. However, no change could be observed in the number of reported occasions of AHC between baseline and one year after the intervention. Health care staff’s participation in workshops using improvised role-play can increase staff’s perceived ability to take action in AHC situations. The voluntary nature of the intervention may have attracted those who were already aware of the topic, and likely explains the unchanged awareness of AHC.
The Smartphone-Assisted Intervention Improved Perception of Nutritional Status among Middle School Students
Authors: Shen Yan-Hui , Liu Zheng , Li Wen-Hao , Zhou Shuang , Xu Jin-Hui , Jiang Chu , Wang Hai-Jun
Published: August 2020

Misperception of nutritional status is common and hinders the progress of childhood obesity prevention. This study aimed to examine the effectiveness of a smartphone-assisted intervention to improve student and parental perception of students’ nutritional status (underweight, normal weight, overweight, obese). We conducted a parallel-group controlled trial with a non-randomized design in three junior middle schools of Beijing, China in 2019. One school was allocated to the intervention group and two schools to the control group. A total of 573 students (aged 13.1 ± 0.4 years) participated in the trial. The 3-month intervention included three components: health education sessions for students and parents, regular monitoring of students’ weight, and the provision of feedback via a smartphone application. Schools in the control group continued their usual practice. Primary outcomes included the student and parental accurate perception of students’ nutritional status. The percentage of students’ accurate perception of their own nutritional status in the intervention group increased from 49.0% to 59.2% from baseline to three months, whereas it decreased from 64.1% to 58.1% in the control group; the adjusted odds ratio (OR) between the two groups was 1.71 (95% confidence interval (CI): 1.13, 2.59). The intervention did not significantly improve parental perception of students’ nutritional status (p > 0.05). The study findings provided a brief approach for improving perception of nutritional status among middle school students.
Analysis of the Impact of the Confinement Resulting from COVID-19 on the Lifestyle and Psychological Wellbeing of Spanish Pregnant Women: An Internet-Based Cross-Sectional Survey
Authors: Biviá-Roig Gemma , La Rosa Valentina Lucia , Gómez-Tébar María , Serrano-Raya Lola , Amer-Cuenca Juan José , Caruso Salvatore , Commodari Elena , Barrasa-Shaw Antonio , Lisón Juan Francisco
Published: August 2020

(1) Background: This study aimed to analyze the impact of the confinement due to the COVID-19 pandemics on the eating, exercise, and quality-of-life habits of pregnant women. (2) Methods: This was an internet-based cross-sectional survey which collected information about adherence to the Mediterranean diet, physical exercise, health-related quality of life (HRQoL), and perceived obstacles (in terms of exercise, preparation for delivery, and medical appointments) of pregnant women before and after the confinement. The survey was conducted in 18–31 May 2020. (3) Results: A total of 90 pregnant women participated in this study. There was a significant decrease in the levels of physical activity (p < 0.01) as well as in HRQoL (p < 0.005). The number of hours spent sitting increased by 50% (p < 0.001), 52.2% were unable to attend delivery preparation sessions because these had been cancelled. However, there were no significant differences in the eating pattern of these women (p = 0.672). Conclusions: These results suggest the need to implement specific online programs to promote exercise and reduce stress, thus improving the HRQoL in this population, should similar confinements need to occur again for any reason in the future.
Greater Engagement with Health Information Is Associated with Adoption and Maintenance of Healthy Lifestyle Behaviours in People with MS
Authors: Lin Xin , Yu Maggie , Jelinek George A. , Simpson-Yap Steve , Neate Sandra , Nag Nupur
Published: August 2020

Health communication offers an important means for patients to make informed decisions for illness self-management. We assessed how the level of engagement with selected health information at baseline is associated with the adoption and maintenance of lifestyle behaviours at a 5-year follow-up in people with multiple sclerosis (MS). Non-engagers were compared to engagers of information delivered online and print (medium), and with engagers who additionally attended a live-in workshop (high). Engagement was assessed against lifestyle behaviours by log-binomial regression. Information engagers had higher education, and were less likely to have severe disability, clinically significant fatigue, or obesity. Medium and high baseline engagement was associated with adopting healthy behaviours for omega 3 supplementation (RR = 1.70; 95%CI: 1.02–2.84), physical activity (RR = 2.16; 95%CI: 1.03–4.55), and dairy non-consumption (RR = 3.98; 95%CI: 1.85–8.56) at 5 years; associations were stronger among high engagers. Only high baseline engagement was associated with maintaining behaviours from baseline to 5 years, specifically for omega-3 (RR = 1.26; 95%CI: 1.06–1.49) and vitamin D supplementation (RR = 1.26; 95%CI: 1.04–1.54) and dairy non-consumption (RR = 1.47; 95%CI: 1.03–2.10). Health communication that includes face-to-face information delivery and practical tools for implementation in daily living may be optimal for adopting and maintaining lifestyle behaviours in people with MS.
Understanding Psychosocial Wellbeing in the Context of Complex and Multidimensional Problems
Authors: Eiroa-Orosa Francisco José
Published: August 2020

This Special Issue deals with the topic of how people and social groups face problems in an increasingly complex and globalized society. The topics included in the call for papers were the interaction of psychosocial well-being and mental health with economic, gender, racial and ethnic inequalities, migration and demographic change and conflict and war, as well as the effects of stigma on people discriminated against because of their differential characteristics, whether they are of a sexual, disability or other minority. We made this proposal because we believed that, despite the introduction of the biopsychosocial model in the late 1970s as a paradigm of the integration of different disciplinary views, research in mental health and psychosocial well-being is still highly fragmented. For decades, we have tried to advance by emphasizing a part of the equation, with results that are at least modest. Therefore, in this Special Issue, we prioritized works aiming at disciplinary and methodological integration. The Special Issue was open to any subject area related to the impacts of social issues on mental health and psychosocial well-being. We were interested in empirical and theoretical enquiries at all ecological levels, from the psychosocial impact of social dynamics on individuals, to the analysis of how sociocultural and geopolitical factors influence health and collective psychosocial well-being.
Detrimental Effect of Perceived Controlling Behavior from Physical Education Teachers on Students’ Leisure-Time Physical Activity Intentions and Behavior: An Application of the Trans-Contextual Model
Authors: Koka Andre , Tilga Henri , Kalajas-Tilga Hanna , Hein Vello , Raudsepp Lennart
Published: August 2020

In the present study, a trans-contextual model was applied to examine the relations between students’ perceptions of controlling behavior from teachers, frustration over their basic psychological needs, autonomous motivation toward physical activity in a physical education context, autonomous motivation toward physical activity in an out-of-school context, beliefs and intentions toward future physical activity, and actual participation in physical activity outside of school. We adopted a three-wave prospective study design in which 234 students aged 11–19 years first completed measures of perceived controlling behavior from teachers, frustration over their basic psychological needs, and autonomous motivation toward physical activity in physical education. One week later, their autonomous motivation, beliefs, and intentions toward physical activity outside of school were measured. Students’ self-reported engagement in physical activity outside of school was assessed another five weeks later. Results of the path analysis revealed a significant and negative indirect effect of perceived controlling behavior from physical education teachers on students’ intention toward physical activity outside of school via the proposed motivational sequence of the trans-contextual model. There was also a significant and negative indirect effect of perceived controlling behavior from physical education teachers on students’ self-reported engagement in physical activity outside of school, mediated by the frustration over their need for competence in physical education. Findings emphasize the importance of decreasing controlling behaviors from teachers in a physical education context so as not to inhibit students’ physical activity behavior in an out-of-school context.
Association of Self-Reported Medication Adherence with Potentially Inappropriate Medications in Elderly Patients: A Cross-Sectional Pilot Study
Authors: Miyazaki Motoyasu , Uchiyama Masanobu , Nakamura Yoshihiko , Matsuo Koichi , Ono Chika , Goto Miwa , Unoki Ayako , Nakashima Akio , Imakyure Osamu
Published: August 2020

Background: Polypharmacy (PP) and potentially inappropriate medications (PIMs) cause problematic drug-related issues in elderly patients; however, little is known about the association between medication adherence and PP and PIMs. This study evaluated the association of self-reported medication adherence with PP and PIMs in elderly patients. Methods: A cross-sectional pilot study was conducted using data collected from electronic medical records of 142 self-administering patients aged ≥65 years, excluding emergency hospitalization cases. Self-reported medication adherence was assessed using the visual analogue scale (VAS). Results: Of the 142 patients, 91 (64.1%) had PP and 80 (56.3%) used at least one PIM. In univariate analysis, patients with a VAS score of 100% had a significantly higher number of female patients and ≥1 PIM use compared to other patients. We found no association between the VAS score and PP. In multivariable analysis, the use of PIMs was significantly associated with a VAS score of 100% (odds ratio = 2.32; 95% confidence interval = 1.16–4.72; p = 0.017). Conclusions: Use of PIMs by elderly patients is significantly associated with self-reported medication adherence. Pharmacists should pay more attention to prescribed medications of self-administering elderly patients in order to improve their prescribing quality.
Influential Factors Affecting Protective Coping Behaviors of Flood Disaster: A Case Study in Shenzhen, China
Authors: Cao Weiwei , Yang Yi , Huang Jing , Sun Dianchen , Liu Gaofeng
Published: August 2020

As the risk of urban flooding increases worldwide, floods seriously endanger the safety of people’s lives and property. Understanding the protective coping behaviors of the public in flood disasters is crucial to the implementation of effective flood mitigation measures and flood risk management. In this study, influential factors affecting protective coping behaviors in the face of flood disasters were identified, and the effects of these factors were discussed as well. Shenzhen City in China was selected as the study area, in which a questionnaire survey of 339 respondents was carried out in three flood-prone districts. Correlation analysis was conducted to preselect potential influential factors. Then, two linear regression models were established to identify main influential factors and to explore the interaction effects of these factors. The results indicated that age, monthly income, flood experience, trust in government and insurance willingness were main influential factors of protective coping behaviors. Trust in government had the highest positive correlation coefficient, while monthly income and age were negatively associated with protective coping behaviors. The interaction between insurance willingness and monthly income jointly affected protective coping behaviors of the public. The findings of this study could help authorities better understand the public’s intention to cope with flood and design effective risk reduction measures, not only for Shenzhen, but also for many other similar cities that facing with the same situation.
A Biomechanical Waist Comfort Model for Manual Material Lifting
Authors: Zhang Yongbao , Ke Jinjing , Wu Xiang , Luo Xiaowei
Published: August 2020

Low back pain (LBP) is a common disorder that affects the working population worldwide. LBP causes more disability than any other conditions all around the world. Most existing studies focus on the occupational physical factors in association with LBP, while few focus on individual factors, especially the lack of quantitative calculation of waist comfort in biomechanics. Based on the physical statistics of Chinese men, this research used human posture analysis (HPA) to establish the waist strength formula and analyzed the waist strength during a manual material handling. It also explored the influence of weight and height of lifting objects on the L5-S1 spinal load. On this basis, a waist comfort model was proposed in combination with the recommended weight limit (RWL) recommended by NIOSH, and the parameter selection and waist comfort value were verified by Jack simulation software. The results show that pulling force of the Erector Spinae of the waist is closely related to the weight and lifting height of the object. Parameter verification and Jack software simulation results show that the force of L5-S1 is less than 3400 N, which proves that the waist force under this posture is acceptable. The developed waist comfort model can be applied to evaluate work risk, to adjust working intensity and powered exoskeleton design, aiming to decrease the prevalence of LBP.
Removal of Total Petroleum Hydrocarbons from Contaminated Soil through Microwave Irradiation
Authors: Cho Kanghee , Myung Eunji , Kim Hyunsoo , Purev Oyunbileg , Park Cheonyoung , Choi Nagchoul
Published: August 2020

In this study, we investigated the removal mechanism of total petroleum hydrocarbons (TPH) from soil by microwave heating. TPH contaminated soil was investigated to determine the desorption behavior of five carbon number-based fractions of TPH. The applied operating microwave power density influenced the final temperature that was reached during heating. For low operating power density applications, microwave effectiveness was limited due to the soil’s dielectric properties, which exhibited a direct relationship with temperature variation. Soil particle distribution could be attributed to permeability, which significantly influenced the evaporation of contaminated soil during the microwave treatment. The results indicate that the activation energy was correlated with the influence of particle size. The removal efficiency of the coarse soil reached 91.1% at 15 min, whereas that of fine soil was low. A total of 30 min had passed, and a removal efficiency of 71.2% was found for the fine soil. Residual TPH concentration was decreased when irradiation time was increased with a removal rate dependent on soil temperature variation. The surface functional groups of the contaminated soil were influenced by microwave irradiation, and changes in the hydrocarbon fraction affected contaminant removal.
Bridge Carbon Emissions and Driving Factors Based on a Life-Cycle Assessment Case Study: Cable-Stayed Bridge over Hun He River in Liaoning, China
Authors: Zhou ZhiWu , Alcalá Julián , Yepes Víctor
Published: August 2020

Due to the rapid growth of the construction industry’s global environmental impact, especially the environmental impact contribution of bridge structures, it is necessary to study the detailed environmental impact of bridges at each stage of the full life cycle, which can provide optimal data support for sustainable development analysis. In this work, the environmental impact case of a three-tower cable-stayed bridge was analyzed through openLCA software, and more than 23,680 groups of data were analyzed using Markov chain and other research methods. It was concluded that the cable-stayed bridge contributed the most to the global warming potential value, which was mainly concentrated in the operation and maintenance phases. The conclusion shows that controlling the exhaust pollution of passing vehicles and improving the durability of building materials were the key to reducing carbon contribution and are also important directions for future research.
Misperception of the Cardiovascular Risk in Patients with Rheumatoid Arthritis
Authors: Alonso-Molero Jéssica , Prieto-Peña Diana , Mendoza Guadalupe , Atienza-Mateo Belén , Corrales Alfonso , González-Gay Miguel Á. , Llorca Javier
Published: August 2020

The risk of cardiovascular (CV) disease and mortality is increased by rheumatoid arthritis (RA). However, data on how RA patients perceive their own CV risk and their adherence to CV prevention factors are scarce. We conducted an observational study on 266 patients with RA to determine whether the perceived CV risk correlates to the objective CV risk, and if it influences their compliance with a Mediterranean diet and physical exercise. The objective CV risk was calculated according to the modified European League Against Rheumatism (EULAR) Systematic Coronary Risk Evaluation (SCORE). The perceived CV risk did not correlate to the objective CV risk. The correlation was even lower when carotid ultrasound was used. Notably, 64.62% of patients miscalculated their CV risk, with 43.08% underestimating it. Classic CV risk factors, carotid ultrasound markers and ESR and CRP showed significant correlation with the objective CV risk. However, only hypertension and RA disease features showed association with the perceived CV risk. Neither the objective CV risk nor the perceived CV risk were associated with the accomplishment of a Mediterranean diet or physical activity. In conclusion, RA patients tend to underestimate their actual CV risk, giving more importance to RA features than to classic CV risk factors. They are not concerned enough about the beneficial effects of physical activity or diet.
Structural Insights into the Mechanism of a Nanobody That Stabilizes PAI-1 and Modulates Its Activity
Authors: Sillen Machteld , Weeks Stephen D. , Strelkov Sergei V. , Declerck Paul J.
Published: August 2020

Plasminogen activator inhibitor-1 (PAI-1) is the main physiological inhibitor of tissue-type (tPA) and urokinase-type (uPA) plasminogen activators (PAs). Apart from being critically involved in fibrinolysis and wound healing, emerging evidence indicates that PAI-1 plays an important role in many diseases, including cardiovascular disease, tissue fibrosis, and cancer. Targeting PAI-1 is therefore a promising therapeutic strategy in PAI-1 related pathologies. Despite ongoing efforts no PAI-1 inhibitors were approved to date for therapeutic use in humans. A better understanding of the molecular mechanisms of PAI-1 inhibition is therefore necessary to guide the rational design of PAI-1 modulators. Here, we present a 1.9 Å crystal structure of PAI-1 in complex with an inhibitory nanobody VHH-s-a93 (Nb93). Structural analysis in combination with biochemical characterization reveals that Nb93 directly interferes with PAI-1/PA complex formation and stabilizes the active conformation of the PAI-1 molecule.
Establishment of the Embryonic Shoot Meristem Involves Activation of Two Classes of Genes with Opposing Functions for Meristem Activities
Authors: Aida Mitsuhiro , Tsubakimoto Yuka , Shimizu Satoko , Ogisu Hiroyuki , Kamiya Masako , Iwamoto Ryosuke , Takeda Seiji , Karim Md , Mizutani Masaharu , Lenhard Michael , Tasaka Masao
Published: August 2020

The shoot meristem, a stem-cell-containing tissue initiated during plant embryogenesis, is responsible for continuous shoot organ production in postembryonic development. Although key regulatory factors including KNOX genes are responsible for stem cell maintenance in the shoot meristem, how the onset of such factors is regulated during embryogenesis is elusive. Here, we present evidence that the two KNOX genes STM and KNAT6 together with the two other regulatory genes BLR and LAS are functionally important downstream genes of CUC1 and CUC2, which are a redundant pair of genes that specify the embryonic shoot organ boundary. Combined expression of STM with any of KNAT6, BLR, and LAS can efficiently rescue the defects of shoot meristem formation and/or separation of cotyledons in cuc1cuc2 double mutants. In addition, CUC1 and CUC2 are also required for the activation of KLU, a cytochrome P450-encoding gene known to restrict organ production, and KLU counteracts STM in the promotion of meristem activity, providing a possible balancing mechanism for shoot meristem maintenance. Together, these results establish the roles for CUC1 and CUC2 in coordinating the activation of two classes of genes with opposite effects on shoot meristem activity.
Lactobacillus Strains Alleviated Hyperlipidemia and Liver Steatosis in Aging Rats via Activation of AMPK
Authors: Lew Lee-Ching , Hor Yan-Yan , Jaafar Mohamad-Hafis , Lau Amy-Sie-Yik , Lee Boon-Kiat , Chuah Li-Oon , Yap Kien-Pong , Azlan Azali , Azzam Ghows , Choi Sy-Bing , Liong Min-Tze
Published: August 2020

In this study, we hypothesized that different strains of Lactobacillus can alleviate hyperlipidemia and liver steatosis via activation of 5′ adenosine monophosphate-activated protein kinase (AMPK), an enzyme that is involved in cellular energy homeostasis, in aged rats. Male rats were fed with a high-fat diet (HFD) and injected with D-galactose daily over 12 weeks to induce aging. Treatments included (n = 6) (i) normal diet (ND), (ii) HFD, (iii) HFD-statin (lovastatin 2 mg/kg/day), (iv) HFD-Lactobacillus fermentum DR9 (10 log CFU/day), (v) HFD-Lactobacillus plantarum DR7 (10 log CFU/day), and (vi) HFD-Lactobacillus reuteri 8513d (10 log CFU/day). Rats administered with statin, DR9, and 8513d reduced serum total cholesterol levels after eight weeks (p < 0.05), while the administration of DR7 reduced serum triglycerides level after 12 weeks (p < 0.05) as compared to the HFD control. A more prominent effect was observed from the administration of DR7, where positive effects were observed, ranging from hepatic gene expressions to liver histology as compared to the control (p < 0.05); downregulation of hepatic lipid synthesis and β-oxidation gene stearoyl-CoA desaturase 1 (SCD1), upregulation of hepatic sterol excretion genes of ATP-binding cassette subfamily G member 5 and 8 (ABCG5 and ABCG8), lesser degree of liver steatosis, and upregulation of hepatic energy metabolisms genes AMPKα1 and AMPKα2. Taken altogether, this study illustrated that the administration of selected Lactobacillus strains led to improved lipid profiles via activation of energy and lipid metabolisms, suggesting the potentials of Lactobacillus as a promising natural intervention for alleviation of cardiovascular and liver diseases.
Anticancer Strategy Targeting Cell Death Regulators: Switching the Mechanism of Anticancer Floxuridine-Induced Cell Death from Necrosis to Apoptosis
Authors: Sato Akira , Hiramoto Akiko , Kim Hye-Sook , Wataya Yusuke
Published: August 2020

Cell death can be broadly characterized as either necrosis or apoptosis, depending on the morphological and biochemical features of the cell itself. We have previously reported that the treatment of mouse mammary carcinoma FM3A cells with the anticancer drug floxuridine (FUdR) induces necrosis in the original clone F28-7 but apoptosis in the variant F28-7-A. We have identified regulators, including heat shock protein 90, lamin-B1, cytokeratin-19, and activating transcription factor 3, of cell death mechanisms by using comprehensive gene and protein expression analyses and a phenotype-screening approach. We also observed that the individual inhibition or knockdown of the identified regulators in F28-7 results in a shift from necrotic to apoptotic morphology. Furthermore, we investigated microRNA (miRNA, miR) expression profiles in sister cell strains F28-7 and F28-7-A using miRNA microarray analyses. We found that several unique miRNAs, miR-351-5p and miR-743a-3p, were expressed at higher levels in F28-7-A than in F28-7. Higher expression of these miRNAs in F28-7 induced by transfecting miR mimics resulted in a switch in the mode of cell death from necrosis to apoptosis. Our findings suggest that the identified cell death regulators may play key roles in the decision of cell death mechanism: necrosis or apoptosis.
A Two-Step Immunomagnetic Microbead-Based Method for the Isolation of Human Primary Skin Telocytes/CD34+ Stromal Cells
Authors: Romano Eloisa , Rosa Irene , Fioretto Bianca Saveria , Lucattelli Elena , Innocenti Marco , Ibba-Manneschi Lidia , Matucci-Cerinic Marco , Manetti Mirko
Published: August 2020

Telocytes (TCs), commonly referred to as TCs/CD34+ stromal cells, are a peculiar type of interstitial cells with distinctive morphologic traits that are supposed to exert several biological functions, including tissue homeostasis regulation, cell-to-cell signaling, immune surveillance, and reparative/regenerative effects. At present, the majority of studies investigating these cells are mainly descriptive and focus only on their morphology, with a consequent paucity of functional data. To gain relevant insight into the possible functions of TCs, in vitro analyses are clearly required, but currently, the protocols for TC isolation are only at the early stages and not fully standardized. In the present in vitro study, we describe a novel methodology for the purification of human primary skin TCs through a two-step immunomagnetic microbead-based cell separation (i.e., negative selection for CD31 followed by positive selection for CD34) capable of discriminating these cells from other connective tissue-resident cells on the basis of their different immunophenotypic features. Our experiments clearly demonstrated that the proposed method allows a selective purification of cells exhibiting the peculiar TC morphology. Isolated TCs displayed very long cytoplasmic extensions with a moniliform silhouette (telopodes) and presented an immunophenotypic profile (CD31−/CD34+/PDGFRα+/vimentin+) that unequivocally differentiates them from endothelial cells (CD31+/CD34+/PDGFRα−/vimentin+) and fibroblasts (CD31−/CD34−/PDGFRα+/vimentin+). This novel methodology for the isolation of TCs lays the groundwork for further research aimed at elucidating their functional properties and possible translational applications, especially in the field of regenerative medicine.
Zooming into the Dark Side of Human Annexin-S100 Complexes: Dynamic Alliance of Flexible Partners
Authors: Weisz Judith , Uversky Vladimir N.
Published: August 2020

Annexins and S100 proteins form two large families of Ca2+-binding proteins. They are quite different both structurally and functionally, with S100 proteins being small (10–12 kDa) acidic regulatory proteins from the EF-hand superfamily of Ca2+-binding proteins, and with annexins being at least three-fold larger (329 ± 12 versus 98 ± 7 residues) and using non-EF-hand-based mechanism for calcium binding. Members of both families have multiple biological roles, being able to bind to a large cohort of partners and possessing a multitude of functions. Furthermore, annexins and S100 proteins can interact with each other in either a Ca2+-dependent or Ca2+-independent manner, forming functional annexin-S100 complexes. Such functional polymorphism and binding indiscrimination are rather unexpected, since structural information is available for many annexins and S100 proteins, which therefore are considered as ordered proteins that should follow the classical “one protein–one structure–one function” model. On the other hand, the ability to be engaged in a wide range of interactions with multiple, often unrelated, binding partners and possess multiple functions represent characteristic features of intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs); i.e., functional proteins or protein regions lacking unique tertiary structures. The aim of this paper is to provide an overview of the functional roles of human annexins and S100 proteins, and to use the protein intrinsic disorder perspective to explain their exceptional multifunctionality and binding promiscuity.
From Ivacaftor to Triple Combination: A Systematic Review of Efficacy and Safety of CFTR Modulators in People with Cystic Fibrosis
Authors: Gramegna Andrea , Contarini Martina , Aliberti Stefano , Casciaro Rosaria , Blasi Francesco , Castellani Carlo
Published: August 2020

Over the last years CFTR (cystic fibrosis transmembrane conductance regulator) modulators have shown the ability to improve relevant clinical outcomes in patients with cystic fibrosis (CF). This review aims at a systematic research of the current evidence on efficacy and tolerability of CFTR modulators for different genetic subsets of patients with CF. Two investigators independently performed the search on PubMed and included phase 2 and 3 clinical trials published in the study period 1 January 2005–31 January 2020. A final pool of 23 papers was included in the systematic review for a total of 4219 patients. For each paper data of interest were extracted and reported in table. In terms of lung function, patients who had the most beneficial effects from CFTR modulation were those patients with one gating mutation receiving IVA (ivacaftor) and patients with p.Phe508del mutation, both homozygous and heterozygous, receiving ELX/TEZ/IVA (elexacaftor/tezacaftor/ivacaftor) had the most relevant beneficial effects in term of lung function, pulmonary exacerbation decrease, and symptom improvement. CFTR modulators showed an overall favorable safety profile. Next steps should aim to systematize our comprehension of scientific data of efficacy and safety coming from real life observational studies.
Association of Electronegative LDL with Macrophage Foam Cell Formation and CD11c Expression in Rheumatoid Arthritis Patients
Authors: Chang Ching-Kun , Chen Po-Ku , Lan Joung-Liang , Chang Shih-Hsin , Hsieh Tsu-Yi , Liao Pei-Jyuan , Chen Chu-Huang , Chen Der-Yuan
Published: August 2020

L5, the most negatively charged subfraction of low-density lipoprotein (LDL), is implicated in atherogenesis, but the pathogenic association is relatively unexplored in patients with rheumatoid arthritis (RA). We examined the role of L5 LDL in macrophage foam cell formation and the association of L5 with CD11c expression in THP-1 cells and RA patients. Using quantitative real-time PCR, we determined mRNA expression levels of ITGAX, the gene for CD11c, a marker associated with vascular plaque formation and M1 macrophages in atherogenesis, in 93 RA patients. We also examined CD11c expression on THP-1 cells treated with L5 by flow cytometry analysis and the plasma levels of inflammatory mediators using a magnetic bead array. We found a dose-dependent upregulation of foam cell formation of macrophages after L5 treatment (mean ± SEM, 12.05 ± 2.35% in L5 (10 µg/mL); 50.13 ± 3.9% in L5 (25 µg/mL); 90.69 ± 1.82% in L5 (50 µg/mL), p < 0.01). Significantly higher levels of CD11c expression were observed in 30 patients with a high percentage of L5 in LDL (L5%) (0.0752 ± 0.0139-fold) compared to 63 patients with normal L5% (0.0446 ± 0.0054-fold, p < 0.05). CD11c expression levels were increased in the L5-treated group (30.00 ± 3.13% in L5 (10 µg/mL); 41.46 ± 2.77% in L5 (50 µg/mL), p < 0.05) and were positively correlated with plasma levels of interleukin (IL)-6 and IL-8. L5 augmented the expression of IL-6, IL-8, and tumor necrosis factor-α (TNF-α) on monocytes and macrophages. Our findings suggest that L5 may promote atherogenesis by augmenting macrophage foam cell formation, upregulating CD11c expression, and enhancing the expression levels of atherosclerosis-related mediators.