bluuebunny/crossref_metadata_2025 huggingface dataset:
An Overview of REBA Method Applications in the World
Authors: Hita-Gutiérrez Manuel , Gómez-Galán Marta , Díaz-Pérez Manuel , Callejón-Ferre Ángel-Jesús
Published: April 2020

The objective of this work is to review literature, worldwide, in which the Rapid Entire Body Assessment (REBA) ergonomic assessment method was applied and count the number of times that REBA was applied together with other methods and subsequent incidence. The database used was the “Web of Science—Core Collection”. Only scientific articles and bibliographic reviews were included, analysing a total of 314 documents and selecting only 91. The use of the REBA method is indicated in terms of knowledge, country, year and journal sectors. It was most used in the knowledge areas of “Manufacturing” (24.18%), “Agriculture, forestry and fishing” (21.98%) and in “Other activities” (19.78%). One of the benefits of REBA is that it evaluates different body parts: upper limbs (arm, forearm and wrist), lower extremities, trunk and neck. It is a useful method to identify the forced postures adopted by workers to thus develop improvement measures if necessary. It is concluded that REBA method use has increased over the last decade, probably due to the digitization of knowledge. It is almost always applied in combination with other methods, and its use can be a positive indicator of company sustainability.
Dental Amalgam Fillings and Multiple Sclerosis: A Nationwide Population-Based Case–Control Study in Taiwan
Authors: Tseng Chien-Fang , Chen Kun-Huang , Yu Hui-Chieh , Huang Fu-Mei , Chang Yu-Chao
Published: April 2020

Multiple sclerosis (MS) is an inflammatory neurological disease characterized by autoimmune-mediated demyelination of the central nervous system. Genetic and environmental factors may contribute to the development of MS. This has not been confirmed yet. Dental amalgam has long been controversial in MS due to its mercury content but the toxicological implications of mercury-containing amalgam fillings (AMF) for MS remain to be elucidated. We conducted a case–control study to investigate the association between AMF and the risk of MS from the Taiwanese National Health Insurance Research Database (NHIRD). Case (n = 612) and control (n = 612) groups were matched by sex, age, urbanization level, monthly income, and Charlson comorbidity index by propensity score matched with a 1:1 ratio from 2000 to 2013. Differences between cases and controls was not statistically significant (OR: 0.82, 95% CI = 0.65–1.05). In subjects stratified by gender, MS was also not associated with AMF for women (OR: 0.743, 95% CI = 0.552–1.000) and men (OR: 1.006, 95% CI = 0.670–1.509), respectively. In summary, this Taiwanese nationwide population-based case–control study did not find an association between MS and AMF.
Impact of Foehn Wind and Related Environmental Variables on the Incidence of Cardiac Events
Authors: Maciejczak Andrzej , Guzik Agnieszka , Wolan-Nieroda Andżelina , Wójcik Marzena , Pop Teresa
Published: April 2020

In Poland there is no data related to the impact of halny wind and the related environmental variables on the incidence of cardiac events. We decided to investigate the relationship between this weather phenomenon, as well as the related environmental variables, and the incidence of cardiac events in the population of southern Poland, a region affected by this type of wind. We also decided to determine whether the environmental changes coincide with or predate the event examined. We analysed data related to 465 patients admitted to the cardiology ward in a large regional hospital during twelve months of 2011 due to acute myocardial infarction. All the patients in the study group lived in areas affected by halny wind and at the time of the event were staying in those areas. The frequency of admissions on halny days did not differ significantly from the admissions on the remaining days of the year (p = 0.496). No statistically significant differences were found between the number of admissions on halny days and on the remaining days during halny months (p = 0.084). We have identified a difference in the number of admissions between days with no halny and days immediately preceding onset of halny (p = 0.001). However, no effects of the related environmental variables have been observed in the incidence of cardiac events (p = 0.866, F = 0.37). On the days with halny wind, incidence of cardiac events is similar to that on the remaining days of the year.
Training and Competition Load in Female Basketball: A Systematic Review
Authors: Reina María , García-Rubio Javier , Ibáñez Sergio J.
Published: April 2020

The scientific literature on women’s basketball is still limited, mainly in performance parameters. The purpose of this study was to analyse the state of art on the internal and external loads supported by female basketball players during sports practice. The design of this research is theoretical. The most relevant databases were searched for pertinent published studies according to the following keywords: “basketball”, “female” or “woman”, “training” or “competition”, “load” or “demand”. Of the 644 studies initially identified, 26 were selected for a complete review. These investigations were characterised by having as an objective an individualization of training for this type of population. Of the selected studies, it was evaluated: (i) goal (training, competition or both); (ii) category (stages: U14, U16, U18 and senior; level: state, national or international); (iii) type of load (Internal, External or both); (iv) instruments used and (v) variables analysed. The most studied goal was competition, mainly in senior national level, carrying out an analysis of the external and internal load together. Depending on the instruments and the variables used, the subjective load analysis was recurrent and important in the publication of articles in this topic. The quality of the studies was good, but for a better description of women’s basketball, there is a need to jointly investigate sports training and competition, to study the training categories and to use micro technology that guarantees obtaining objective and reliable data.
Process Evaluation of a Nutrition and Lifestyle Behavior Peer Support Program for Adults with Metabolic Syndrome
Authors: Mahadzir Muhammad Daniel Azlan , Quek Kia Fatt , Ramadas Amutha
Published: April 2020

Metabolic Syndrome (MetS) is a cluster of risk factors that increases the risk for diabetes and cardiovascular diseases. Lifestyle intervention is the gold standard of MetS management and prevention. Despite the growing positive influence of peer support-based interventions on management of various chronic diseases, its potential among adults with MetS has not been elucidated. We describe the development and process evaluation of a nutrition and lifestyle behavior “PEeR SUpport program for ADults with mEtabolic syndrome” (PERSUADE) using a systematic five-step approach—(i) review of evidence; (ii) focus group discussions; (iii) behavioral matrix development; (iv) module development; and (v) feasibility and process evaluation. High program adherence was recorded with 81.3% of participants attending all peer sessions. Participants’ content satisfaction score was high (93.3%) while peer leadership score was satisfactory (70.0%). There were significant reductions in all anthropometric and metabolic parameters assessed post intervention, except for diastolic blood pressure. Significant correlations were found between reductions in body fat and triglyceride, and content satisfaction. Peer leadership was only significantly correlated with reduction in triglyceride. Future studies can explore aspects of module interactivity, use of social media, and other means to stimulate consistent engagement of participants, as well as extending the implementations to other lifestyle-related diseases.
Modes of Early Detection of Breast Cancer in Katowice Region, Poland
Authors: Zejda Jan Eugeniusz , Kaleta Angelina
Published: April 2020

Background: Our 2004 survey of breast cancer survivors in the Katowice region (Poland) showed that the detection of the disease was triggered by self-examination in 58.9%, mammography in 19.2%, and clinical examination in 19.7% of cases. The purpose of the current study (2019/2020) was to determine if the implementation of national screening (mammography) in 2007 resulted in an increase of the relative contribution of mammography to detection of cancer. Methods: Subjects were 215 breast cancer patients, members of self-support groups in Katowice region. The questionnaire included questions on early detection of breast cancer, participation in screening, and socio-economic status. Results: Early detection of cancer was initiated by self-examination in 63.7%, mammography in 22.8%, clinical examination in 13.5% of subjects. Age at detection depended on the method (p < 0.001): 54.1 ± 10.8 years for self-examination, 60.0 ± 7.6 years for mammography, and 58.7 ± 10.8 years for clinical examination. Conclusions: Both in 2004 and 2020 self-examination is the most frequent method of early detection of breast cancer in the study area. The contribution of mammography remains on a low level (23%). This finding could be explained by a low participation in screening and by age of 50 years used as the entry criterion to national screening of breast cancer in Poland.
The Role of Microbiome, Dietary Supplements, and Probiotics in Autism Spectrum Disorder
Authors: Sivamaruthi Bhagavathi Sundaram , Suganthy Natarajan , Kesika Periyanaina , Chaiyasut Chaiyavat
Published: April 2020

Autism spectrum disorder (ASD) is a serious neurodevelopmental disorder characterized by the impairment of the cognitive function of a child. Studies suggested that the intestinal microbiota has a critical role in the function and regulation of the central nervous system, neuroimmune system and neuroendocrine system. Any adverse changes in the gut–brain axis may cause serious disease. Food preferences and dietary patterns are considered as key in influencing the factors of ASD development. Several recent reviews narrated the importance of dietary composition on controlling or reducing the ASD symptoms. It has been known that the consumption of probiotics confers several health benefits by positive amendment of gut microbiota. The influence of probiotic intervention in children with ASD has also been reported and it has been considered as an alternative and complementary therapeutic supplement for ASD. The present manuscript discusses the role of microbiota and diet in the development of ASD. It also summarizes the recent updates on the influence of dietary supplements and the beneficial effect of probiotics on ASD symptoms. An in-depth literature survey suggested that the maternal diet and lifestyle are greatly associated with the development of ASD and other neurodevelopmental disorders. Mounting evidences have confirmed the alteration in the gut microbial composition in children suffering from ASD. However, the unique profile of microbiome has not yet been fully characterized due to the heterogeneity of patients. The supplementation of probiotics amended the symptoms associated with ASD but the results are inconclusive. The current study recommends further detailed research considering the role of microbiome, diet and probiotics in the development and control of ASD.
Histological Effects of an Innovative 445 Nm Blue Laser During Oral Soft Tissue Biopsy
Authors: Palaia Gaspare , Pergolini Daniele , D’Alessandro Leonardo , Carletti Raffaella , Del Vecchio Alessandro , Tenore Gianluca , Di Gioia Cira Rosaria Tiziana , Romeo Umberto
Published: April 2020

Continuously evolving laser devices can be used in various fields; they are an alternative to the traditional cold blade surgery to perform biopsies of oral soft tissues. The aspect focused on in this paper is the possibility to use the 445 nm diode laser (Eltech K-Laser srl, Treviso, Italy) in complete safety, by evaluating its thermal effects during microscopy. A histological evaluation of the alteration of the peri-incisional edges on 10 samples was realized. All excisional biopsies were related to clinically unsuspected lesions and performed by the same expert operator. The surgical procedure was performed with the same laser parameters and the same pathologist evaluated the thermal effect on the samples. An average value of the detected tissue alteration was calculated; the average damage of the epithelium was 650.93 μm, while in the connective tissue it was 468.07 μm. In all the cases a clear diagnosis was possible, and no clinical complications were observed; so, the 445 nm diode laser proved to be a device that can be safely used for biopsies of clinically unsuspicious lesions. Due to the small number of cases, this in vivo preliminary experience needs to be extended.
Bisphenols as Environmental Triggers of Thyroid Dysfunction: Clues and Evidence
Authors: Gorini Francesca , Bustaffa Elisa , Coi Alessio , Iervasi Giorgio , Bianchi Fabrizio
Published: April 2020

Bisphenols (BPs), and especially bisphenol A (BPA), are known endocrine disruptors (EDCs), capable of interfering with estrogen and androgen activities, as well as being suspected of other health outcomes. Given the crucial role of thyroid hormones and the increasing incidence of thyroid carcinoma in the last few decades, this review analyzes the effects of BPS on the thyroid, considering original research in vitro, in vivo, and in humans published from January 2000 to October 2019. Both in vitro and in vivo studies reported the ability of BPs to disrupt thyroid function through multiple mechanisms. The antagonism with thyroid receptors (TRs), which affects TR-mediated transcriptional activity, the direct action of BPs on gene expression at the thyroid and the pituitary level, the competitive binding with thyroid transport proteins, and the induction of toxicity in several cell lines are likely the main mechanisms leading to thyroid dysfunction. In humans, results are more contradictory, though some evidence suggests the potential of BPs in increasing the risk of thyroid nodules. A standardized methodology in toxicological studies and prospective epidemiological studies with individual exposure assessments are warranted to evaluate the pathophysiology resulting in the damage and to establish the temporal relationship between markers of exposure and long-term effects.
Air-Pollution Control in an Emergent Market: Does It Work? Evidence from Romania
Authors: Oncioiu Ionica , Dănescu Tatiana , Popa Maria-Alexandra
Published: April 2020

Economic development in a national and international context must be based on a sustainability strategy established on the systemic interaction between the economic, sociocultural, and ecological environments. Today, the world is confronted by many challenges related to climate change and natural-resource flows, including waste streams resulting from economic activity. The need for national and European environmental standards and the work of an environment monitoring authority to reduce air pollution are highlighted by economic and industrial activities. Thus, our research focused on determining if emissions of sulfur dioxide (SO2), nitrogen (NO2), and particulate matter 10 (PM10) are influenced by planned and unplanned inspections made by competent authorities from Romania. We built a regression model that estimates the influence of economic measures imposed by the authorities on reducing industrial air pollution. Preliminary results showed that the number of inspections negatively influences air pollution, indicating that national and local authorities in Romania are striving to maintain air quality and are conducting more inspections when air pollution is high.
U-Shaped Association between Sleep Duration, C-Reactive Protein, and Uric Acid in Korean Women
Authors: Lee Yea-Chan , Son Da-Hye , Kwon Yu-Jin
Published: April 2020

Serum high-sensitivity C-reactive protein (hsCRP) and serum uric acid (SUA) are biomarkers that predict chronic inflammation and cardiovascular dysfunction. Therefore, we aimed to investigate the association between sleep duration, hsCRP, and SUA in Korean women. Cross-sectional data from the Seventh Korea National Health and Nutrition Examination Survey was analyzed. The odds ratio (OR) and 95% confidence intervals (CIs) for an association between higher hsCRP (>2.0 mg/L) or higher SUA (>5.6 mg/dL) and sleep duration were calculated using multiple logistic regression analyses after adjusting for potential confounders. In total, 6151 women were included in the analysis. There was a U-shaped relationship between continuous sleep duration, hsCRP, and SUA. Compared to those who slept for 7–8 h, the ORs (95% CIs) for higher hsCRP were 1.43 (0.95–2.16) in short sleepers and 1.64 (1.09–2.48) in long sleepers after adjusting for confounders. Compared with those who slept for 7–8 h, the ORs (95% CIs) for higher SUA were 1.54 (1.04–2.26) in short sleepers and 1.94 (1.27–2.96) in long sleepers after adjusting for confounders. We found a U-shaped association between sleep duration, hsCRP, and SUA in Korean women. 7–8 h sleep was associated with lower level of hsCRP and SUA in Korean women.
Potential Savings in DHW Facilities through the Use of Solar Thermal Energy in the Hospitals of Extremadura (Spain)
Authors: Sánchez-Barroso Gonzalo , González-Domínguez Jaime , García-Sanz-Calcedo Justo
Published: April 2020

Hospitals need to prepare large amounts of domestic hot water (DHW) to develop their healthcare activity. The aim of this work was to analyse potential savings that can be achieved by installing solar thermal energy for production of domestic hot water in the hospitals of Extremadura (Spain). For this purpose, 25 hospitals between 533 and 87,118 m2 and between 15 and 529 beds were studied, three solar factor scenarios were simulated (0.70, 0.75 and 0.80) and the necessary investment and corresponding economic and environmental savings were calculated. Better economic results and energy ratios for 70% of solar contribution were obtained. These results show an average payback of 4.74 years (SD = 0.26) reaching 4.29 kWh/€ per year (SD = 0.20). Undertaking an investment of 674,423 €, 2,895,416 kWh/year of thermal energy could be generated with which to save both 145,933 € and 638 tons of CO2 per year. It was statistically demonstrated the priority of carrying out an installation with a solar factor of 70%, investing preferably in hospitals in Cáceres over those in Badajoz, especially in the public sector with more than 300 beds. These findings will provide hospital managers with useful information to make decisions on future investments.
A Method of Constructing Marine Oil Spill Scenarios from Flat Text Based on Semantic Analysis
Authors: Huang Kui , Nie Wen , Luo Nianxue
Published: April 2020

Constructed emergency response scenarios provide a basis for decision makers to make management decisions, and the development of such scenarios considers earlier historical cases. Over the decades, the development of emergency response scenarios has mainly implemented the elements of historic cases to describe the grade and influence of an accident. This paper focuses on scenario construction and proposes a corresponding framework based on natural language processing (NLP) using text reports of marine oil spill accidents. For each accident, the original textual reports are first divided into sentence sets corresponding to the temporal evolution. Each sentence set is regarded as a textual description of a marine oil spill scenario. A method is proposed in this paper, based on parsing, named entity recognition (NER) and open information extraction (OpenIE) to process the relation triples that are extracted from the sentence sets. Finally, the relation triples are semantically clustered into different marine oil spill domains to construct scenarios. The research results are validated and indicate that the proposed scenario construction framework can be effectively used in practical applications.
The Being Taken Seriously Questionnaire—Development and Psychometric Evaluation of a PREM Measure for Person-Centeredness in a High-Tech Hospital Environment
Authors: Forsberg Anna , Rantala Andreas
Published: April 2020

There is a need for patient-reported experience measures (PREMs) in high-tech hospital environments based on the philosophy of person-centeredness. The aim of this study was to describe the development and initial psychometric evaluation of a measure of person-centeredness by means of being taking seriously. In this cross-sectional survey, the study sample (n = 79) completed two measures, the Being Taken Seriously Questionnaire—Patient version (BTSQ-P) which was the measure undergoing psychometric evaluation, and the Person-Centered Climate Questionnaire—Patient version (PCQ-P) that was used to evaluate the climate in which the intervention was occurring. The expected scale dimensionality was examined both by the confirmatory multi-trait analysis program and by explorative principal component analysis (with oblique, varimax rotation). Scale reliability was estimated using ordinal alpha and Cronbach’s alpha. One solid factor was generated. This factor had good internal convergent validity and good item–scale reliability, covering 80.41% of the variance. The interitem correlation ranged from 0.759 to 0.908 and the alpha was 0.93 (ordinal alpha) and 0.87 (Cronbach’s alpha) respectively. There was a strong relationship between BTSQ-P and the PCQ-P dimension safety climate. In conclusion, the internal consistency, reliability and concurrent validity of the BTSQ-P was satisfactory for use in a high-tech hospital environment.
Does the Conversion of Household Registration Actually Improve the Happiness of Migrant Workers in China?
Authors: Liu Xin-hao , Han Li-min , Yuan Bin
Published: April 2020

Migrant workers are an important human resource for economic and social development. Considering the government’s goal of serving and improving people’s livelihoods, improving the happiness of migrant workers is necessary. This study investigates in-depth the impact of the conversion of household registration on migrant workers’ happiness, which is represented by a multi-dimensional comprehensive index based on the propensity matching score model and data from the China Migrants Dynamic Survey (CMDS) in 2017. Moreover, this study explores the different effects of conversion among the groups divided by the characteristics of migrant workers. The results show that from an overall perspective, although the conversion of household registration could improve the happiness of migrant workers, the degree of this improvement is minor. Further, the characteristics of the different groups, including age, educational background, contracted land, collective dividends, and income significantly affect the improvement of happiness. The conversion of household registration has obviously improved the happiness of migrant workers with low educational backgrounds, low income, and contracted land. Based on these findings, the government should take more targeted actions to improve the positive effects of household registration among different migrant worker groups due to the different characteristics in the process of household registration system reform.
Effects of a Wireless Charging System Built for An Electric Kick Scooter on Human Biological Tissue
Authors: Dergham Ibrahim , Alayli Yasser , Imad Rodrigue , Hamam Yskandar
Published: April 2020

In this paper, the authors present an evaluation of the electromagnetic fields generated by a static wireless charging system designed for an electric kick scooter on the human biological tissue. The guidelines on the exposure to the electromagnetic fields are previously specified. In this work, a model is designed under COMSOL multi-physics to study the effects of the magnetic field on two possible body parts of a person, which might be exposed to this field, namely the head and the hands is analysed. The magnetic flux density, the induced electrical field, the specific absorption rate, and the resulting increase of temperature of biological tissues are modelled and compared to the limits and guidelines prescribed in the regulation established to limit the exposure of people to electromagnetic fields. Furthermore, the used wireless charging system is modified to operate at higher frequencies to study its effect. The obtained results are below the guidelines and limits of exposure to the electromagnetic fields specified by the International Commission on Non-Ionizing Radiation Protection, European Commission, Institute of Electrical and Electronics Engineers and International Electrotechnical Commission.
Resistance Training and Handball Players’ Isokinetic, Isometric and Maximal Strength, Muscle Power and Throwing Ball Velocity: A Systematic Review and Meta-Analysis
Authors: Bragazzi Nicola Luigi , Rouissi Mehdi , Hermassi Souhail , Chamari Karim
Published: April 2020

Purpose: Handball (Team Handball) is an intermittent and strenuous contact sport, the successful performance of which depends on frequent body contacts, and the ability to make repeated explosive muscular contractions required for jumping, acceleration, sprinting, turning, changing pace, and throwing a ball. Many studies have investigated the effect of resistance training (RT) in handball players, however with conflicting results. Therefore, our objective was to investigate the impact of RT on maximal strength (isometric and isokinetic strength), the power of both lower and upper limbs, and throwing velocity, in handball players. Methods: A comprehensive literature search yielded a pool of 18 studies, which were retained in the systematic review and meta-analysis. Results: A total of 275 handball players were included. The overall effect size (ES) of RT was 0.996 ([95%CI 0.827–1.165], p = 0.0000). At the multivariate meta-regression, the effect of publication year was significant, as well as the effects of country, gender, and level. The impact of RT on isokinetic strength was not significant (ES 0.079 [95%CI −0.060–0.219], p = 0.265), whereas the impact of RT on throwing (ES 1.360 [95%CI 0.992–1.728], p = 0.000) was significant, as well as the effects of RT on isometric strength (ES 0.398 [95%CI 0.096–0.700], p = 0.010), on maximal strength (ES 1.824 [95%CI 1.305–2.343], p = 0.000), and on power (ES 0.892 [95%CI 0.656–1.128], p = 0.000). Conclusions: RT has a significant impact in handball players. Handball coaches could design conditioning protocols and programs based on our results. However, due to a number of shortcomings, including the high, statistically significant heterogeneity among studies and the evidence of publication bias, further high-quality investigations are needed.
A Systematic Review of the Effectiveness of Concussion Education Programs for Coaches and Parents of Youth Athletes
Authors: Feiss Robyn , Lutz Molly , Reiche Elaine , Moody Justin , Pangelinan Melissa
Published: April 2020

Coach and parent concussion education programs are essential for the prevention, diagnosis, management, and return to play of youth athletes. This systematic review examined the content and efficacy (changes in knowledge, impact on concussion incidence) of concussion education programs for coaches and parents of youth and high school athletes. Six databases were searched: SPORTDiscus, Academic Search Premiere, CINAHL, PsycINFO, PubMed, and Google Scholar. Studies evaluated the use and/or efficacy of concussion education programs among coaches or parents of youth athletes. A total of 13 articles (out of 1553 articles) met selection criteria. Although different concussion education programs exist, only three have been evaluated in the literature: ACTive Athletic Concussion Training™, USA Football’s Heads Up Football, and the Center for Disease Control and Prevention’s HEADS UP. These programs are well liked among coaches and parents and the suggested practices are easily implemented by coaches. These programs increased concussion knowledge among coaches and parents and promoted behavioral changes among coaches to reduce the concussion risk in high school sports. Few studies have assessed the efficacy of concussion education programs on youth athlete health outcomes. No studies included a longitudinal follow up to determine the degree of knowledge retention following the intervention. While online educational programs are sufficient to improve coach knowledge, in-person training may be a more effective educational tool for reducing the incidence of youth sport concussion. Future studies addressing the efficacy of concussion education programs should include a longitudinal follow up to assess knowledge retention and fidelity.
Living with Chronic Heart Failure: Exploring Patient, Informal Caregiver, and Healthcare Professional Perceptions
Authors: Sedlar Natasa , Lainscak Mitja , Farkas Jerneja
Published: April 2020

Due to the complexity of heart failure (HF) and its treatment process, a high level of patient and informal caregiver engagement is required for management results. We aimed to explore the views of HF patients, informal caregivers, and healthcare professionals about personal experiences, perceived needs, and barriers to optimal HF management. A qualitative study using semi-structured interviews with HF patients (n = 32), their informal caregivers (n = 21), and healthcare professionals (n = 5) was conducted in the outpatient HF clinic in Slovenia in 2018. A content analysis method was used to analyze the data. Negative emotional response to disease and its limitations (especially the inability to continue with work) and changes in family roles were the most prevalent topics regarding the impact of HF on livelihood. Among the most common barriers to HF self-care, were the difficulties in changing lifestyle, financial difficulties due to the disease, traditional cuisine/lack of knowledge regarding a healthy diet and lack of self-confidence regarding physical activity. Despite psychological and social difficulties due to HF being highlighted by patients and informal caregivers, only healthcare professionals emphasized the need to address psychosocial aspects of care in HF management. Established differences could inform the implementation of necessary support mechanisms in HF management.
Plantar Pressure Distribution in Female Olympic-Style Weightlifters
Authors: Hawrylak Arletta , Gronowska Hanna
Published: April 2020

Background: The aim of this study was to investigate differences in static and dynamic plantar pressure and other foot geometry variables between female Olympic-style weightlifters and an age- and sex-matched control group. Methods: The study involved 24 national-level competitive weightlifters and 24 physical education students. Leg dominance was determined and baropodometry was used to assess plantar pressure and foot posture during quiet standing and walking. For all variables basic descriptive statistics were calculated (mean ± standard deviation). Student’s t test was used to compare the between-group differences. Spearman’s rank correlation coefficients were calculated to determine the association between BMI (Body Mass Index) and average and peak plantar pressure. Results: No significant differences were observed in plantar pressure distribution between the two groups. A moderate correlation was found between BMI and non-dominant limb peak and average plantar pressure in the group of weightlifters in the static condition and in the control group in the dynamic condition. Conclusions: Olympic-style weightlifting may affect plantar pressure distribution and foot posture in female weightlifters. BMI may also correlate with peak and average plantar pressure in this population. Further research is needed to determine if Olympic-style weightlifting may affect plantar pressure distribution and foot posture in female weightlifters.
Validation of a Questionnaire Developed to Evaluate a Pediatric eHealth Website for Parents
Authors: Nievas Soriano Bruno José , García Duarte Sonia , Fernández Alonso Ana María , Bonillo Perales Antonio , Parrón Carreño Tesifón
Published: April 2020

There is a need for health professionals to provide parents with not only evidence-based child health websites but also instruments to evaluate them. The main aim of this research was to develop a questionnaire for measuring users’ evaluation of the usability, utility, confidence, the well-child section, and the accessibility of a Spanish pediatric eHealth website for parents. We further sought to evaluate the content validity and psychometric reliability of the instrument. A content validation study by expert review was performed, and the questionnaire was pilot tested. Psychometric analyses were used to establish scales through exploratory and confirmatory factor analyses. Reliability studies were performed using Cronbach’s alpha and two split-half methods. The content validation of the questionnaire by experts was considered as excellent. The pilot web survey was completed by 516 participants. The exploratory factor analysis excluded 27 of the 41 qualitative initial items. The confirmatory factor analysis of the resultant 14-item questionnaire confirmed the five initial domains detected in the exploratory confirmatory analysis. The goodness of fit for the competing models was established through fit indices and confirmed the previously established domains. Adequate internal consistency was found for each of the subscales as well as the overall scale.
An Improved Parallelized Multi-Objective Optimization Method for Complex Geographical Spatial Sampling: AMOSA-II
Authors: Li Xiaolan , Gao Bingbo , Bai Zhongke , Pan Yuchun , Gao Yunbing
Published: April 2020

Complex geographical spatial sampling usually encounters various multi-objective optimization problems, for which effective multi-objective optimization algorithms are much needed to help advance the field. To improve the computational efficiency of the multi-objective optimization process, the archived multi-objective simulated annealing (AMOSA)-II method is proposed as an improved parallelized multi-objective optimization method for complex geographical spatial sampling. Based on the AMOSA method, multiple Markov chains are used to extend the traditional single Markov chain; multi-core parallelization technology is employed based on multi-Markov chains. The tabu-archive constraint is designed to avoid repeated searches for optimal solutions. Two cases were investigated: one with six typical traditional test problems, and the other for soil spatial sampling optimization applications. Six performance indices of the two cases were analyzed—computational time, convergence, purity, spacing, min-spacing and displacement. The results revealed that AMOSA-II performed better which was more effective in obtaining preferable optimal solutions compared with AMOSA and NSGA-II. AMOSA-II can be treated as a feasible means to apply in other complex geographical spatial sampling optimizations.
Genes and Diet in the Prevention of Chronic Diseases in Future Generations
Authors: Franzago Marica , Santurbano Daniele , Vitacolonna Ester , Stuppia Liborio
Published: April 2020

Nutrition is a modifiable key factor that is able to interact with both the genome and epigenome to influence human health and fertility. In particular, specific genetic variants can influence the response to dietary components and nutrient requirements, and conversely, the diet itself is able to modulate gene expression. In this context and the era of precision medicine, nutrigenetic and nutrigenomic studies offer significant opportunities to improve the prevention of metabolic disturbances, such as Type 2 diabetes, gestational diabetes, hypertension, and cardiovascular diseases, even with transgenerational effects. The present review takes into account the interactions between diet, genes and human health, and provides an overview of the role of nutrigenetics, nutrigenomics and epigenetics in the prevention of non-communicable diseases. Moreover, we focus our attention on the mechanism of intergenerational or transgenerational transmission of the susceptibility to metabolic disturbances, and underline that the reversibility of epigenetic modifications through dietary intervention could counteract perturbations induced by lifestyle and environmental factors.
Underlying Ossification Phenotype in a Murine Model of Metastatic Synovial Sarcoma
Authors: Kirkham Matthew , Kalivas Austen , Fatema Kaniz , Luelling Sarah , Dubansky Brooke H. , Dubansky Benjamin , Jones Kevin B. , Barrott Jared J.
Published: April 2020

Synovial sarcoma, an uncommon cancer, typically affects young adults. Survival rates range from 36% to 76%, decreasing significantly when metastases are present. Synovial sarcomas form in soft tissues, often near bones, with about 10% demonstrating ossification in the tumor. The literature is inconclusive on whether the presence of ossification portends a worse prognosis. To this end, we analyzed our genetic mouse models of synovial sarcoma to determine the extent of ossification in the tumors and its relationship with morbidity. We noted higher ossification within our metastatic mouse model of synovial sarcoma. Not only did we observe ossification within the tumors at a frequency of 7%, but an even higher frequency, 72%, of bone reactivity was detected by radiography. An enrichment of bone development genes was associated with primary tumors, even in the absence of an ossification phenotype. In spite of the ossification being intricately linked with the metastatic model, the presence of ossification was not associated with a faster or worse morbidity in the mice. Our conclusion is that both metastasis and ossification are dependent on time, but that they are independent of one another.
Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings
Authors: Akhtar Muhammad Asim , Ilyas Kanwal , Dlouhý Ivo , Siska Filip , Boccaccini Aldo R.
Published: April 2020

Bacterial infection associated with medical implants is a major threat to healthcare. This work reports the fabrication of Copper(II)–Chitosan (Cu(II)–CS) complex coatings deposited by electrophoretic deposition (EPD) as potential antibacterial candidate to combat microorganisms to reduce implant related infections. The successful deposition of Cu(II)–CS complex coatings on stainless steel was confirmed by physicochemical characterizations. Morphological and elemental analyses by scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy verified the uniform distribution of copper in the Chitosan (CS) matrix. Moreover, homogeneous coatings without precipitation of metallic copper were confirmed by X-ray diffraction (XRD) spectroscopy and SEM micrographs. Controlled swelling behavior depicted the chelation of copper with polysaccharide chains that is key to the stability of Cu(II)–CS coatings. All investigated systems exhibited stable degradation rate in phosphate buffered saline (PBS)–lysozyme solution within seven days of incubation. The coatings presented higher mechanical properties with the increase in Cu(II) concentration. The crack-free coatings showed mildly hydrophobic behavior. Antibacterial assays were performed using both Gram-positive and Gram-negative bacteria. Outstanding antibacterial properties of the coatings were confirmed. After 24 h of incubation, cell studies of coatings confirms that up to a certain threshold concentration of Cu(II) were not cytotoxic to human osteoblast-like cells. Overall, our results show that uniform and homogeneous Cu(II)–CS coatings with good antibacterial and enhanced mechanical stability could be successfully deposited by EPD. Such antibiotic-free antibacterial coatings are potential candidates for biomedical implants.
Correction:Chlamydia psittaci PmpD-N Modulated Chicken Macrophage Function by Triggering Th2 Polarization and the TLR2/MyD88/NF-κB Signaling Pathway
Authors: Chu Jun , Li Xiaohui , Qu Guanggang , Wang Yihui , Li Qiang , Guo Yongxia , Hou Lei , Liu Jue , Eko Francis O. , He Cheng
Published: April 2020

The authors would like to make the following corrections to their paper, published in the International Journal of Molecular Sciences [...]
Novel Urethane-Dimethacrylate Monomers and Compositions for Use as Matrices in Dental Restorative Materials
Authors: Barszczewska-Rybarek Izabela M. , Chrószcz Marta W. , Chladek Grzegorz
Published: April 2020

In this study, novel urethane-dimethacrylate monomers were synthesized from 1,3-bis(1-isocyanato-1-methylethyl)benzene (MEBDI) and oligoethylene glycols monomethacrylates, containing one to three oxyethylene groups. They can potentially be utilized as matrices in dental restorative materials. The obtained monomers were used to prepare four new formulations. Two of them were solely composed of the MEBDI-based monomers. In a second pair, a monomer based on triethylene glycol monomethacrylate, used in 20 wt.%, was replaced with triethylene glycol dimethacrylate (TEGDMA), a reactive diluent typically used in dental materials. For comparison purposes, two formulations, using typical dental dimethacrylates (bisphenol A glycerolate dimethacrylate (Bis-GMA), urethane-dimethacrylate (UDMA) and TEGDMA) were prepared. The monomers and mixtures were tested for the viscosity and density. The homopolymers and copolymers, obtained via photopolymerization, were tested for the degree of conversion, polymerization shrinkage, water sorption and solubility, hardness, flexural strength and modulus. The newly developed formulations achieved promising physico-chemical and mechanical characteristics so as to be suitable for applications as dental composite matrices. A combination of the MEBDI-based urethane-dimethacrylates with TEGDMA resulted in copolymers with a high degree of conversion, low polymerization shrinkage, low water sorption and water solubility, and good mechanical properties. These parameters showed an improvement in relation to currently used dental formulations.
CD44 Can Compensate for IgSF11 Deficiency by Associating with the Scaffold Protein PSD-95 during Osteoclast Differentiation
Authors: Kim Hyunsoo , Takegahara Noriko , Walsh Matthew C. , Choi Yongwon
Published: April 2020

Differentiation of osteoclasts, which are specialized multinucleated macrophages capable of bone resorption, is driven primarily by receptor activator of NF-κB ligand (RANKL). Additional signaling from cell surface receptors, such as cell adhesion molecules (CAMs), is also required for osteoclast maturation. Previously, we have demonstrated that immunoglobulin superfamily 11 (IgSF11), a member of the immunoglobulin-CAM (IgCAM) family, plays an important role in osteoclast differentiation through association with the scaffold protein postsynaptic density protein 95 (PSD-95). Here, we demonstrate that the osteoclast-expressed CAM CD44 can compensate for IgSF11 deficiency when cell–cell interaction conditions are suboptimal by associating with PSD-95. Impaired osteoclast differentiation in IgSF11-deficient (IgSF11−/−) cultures was rescued by antibody-mediated stimulation of CD44 or by treatment with low-molecular-weight hyaluronan (LMW-HA), a CD44 ligand. Biochemical analysis revealed that PSD-95, which is required for osteoclast differentiation, associates with CD44 in osteoclasts regardless of the presence or absence of IgSF11. RNAi-mediated knockdown of PSD-95 abrogated the effects of either CD44 stimulation or LMW-HA treatment on osteoclast differentiation, suggesting that CD44, similar to IgSF11, is functionally associated with PSD-95 during osteoclast differentiation. Taken together, these results reveal that CD44 can compensate for IgSF11 deficiency in osteoclasts through association with PSD-95.
Karyopherin α-2 Mediates MDC1 Nuclear Import through a Functional Nuclear Localization Signal in the tBRCT Domain of MDC1
Authors: Radhakrishnan Kamalakannan , Park Seon-Joo , Kim Seok Won , Hariharasudhan Gurusamy , Jeong Seo-Yeon , Chang In Youb , Lee Jung-Hee
Published: April 2020

Mediator of DNA damage checkpoint protein 1 (MDC1) plays a vital role in DNA damage response (DDR) by coordinating the repair of double strand breaks (DSBs). Here, we identified a novel interaction between MDC1 and karyopherin α-2 (KPNA2), a nucleocytoplasmic transport adaptor, and showed that KPNA2 is necessary for MDC1 nuclear import. Thereafter, we identified a functional nuclear localization signal (NLS) between amino acid residues 1989–1994 of the two Breast Cancer 1 (BRCA1) carboxyl-terminal (tBRCT) domain of MDC1 and demonstrated disruption of this NLS impaired interaction between MDC1 and KPNA2 and reduced nuclear localization of MDC1. In KPNA2-depleted cells, the recruitment of MDC1, along with the downstream signaling p roteins Ring Finger Protein 8 (RNF8), 53BP1-binding protein 1 (53BP1), BRCA1, and Ring Finger Protein 168 (RNF168), to DNA damage sites was abolished. Additionally, KPNA2-depleted cells had a decreased rate of homologous recombination (HR) repair. Our data suggest that KPNA2-mediated MDC1 nuclear import is important for DDR signaling and DSB repair.
Functional Analysis of IRF1 Reveals its Role in the Activation of the Type I IFN Pathway in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
Authors: Zhu Ke-Cheng , Zhang Nan , Liu Bao-Suo , Guo Liang , Guo Hua-Yang , Jiang Shi-Gui , Zhang Dian-Chang
Published: April 2020

Interferon (IFN) regulatory factor 1 (IRF1), a transcription factor with a novel helix–turn–helix DNA-binding domain, plays a crucial role in innate immunity by regulating the type I IFN signaling pathway. However, the regulatory mechanism through which IRF1 regulates type I IFN in fish is not yet elucidated. In the present study, IRF1 was characterized from golden pompano, Trachinotus ovatus (designated ToIRF1), and its immune function was identified to elucidate the transcriptional regulatory mechanism of ToIFNa3. The full-length complementary DNA (cDNA) of IRF1 is 1763 bp, including a 900-bp open reading frame (ORF) encoding a 299-amino-acid polypeptide. The putative protein sequence has 42.7–71.7% identity to fish IRF1 and possesses a representative conserved domain (a DNA-binding domain (DBD) at the N-terminus). The genomic DNA sequence of ToIRF1 consists of eight exons and seven introns. Moreover, ToIRF1 is constitutively expressed in all examined tissues, with higher levels being observed in immune-relevant tissues (whole blood, gill, and skin). Additionally, Cryptocaryon irritans challenge in vivo increases ToIRF1 expression in the skin as determined by Western blotting (WB); however, protein levels of ToIRF1 in the gill did not change significantly. The subcellular localization indicates that ToIRF1 is localized in the nucleus and cytoplasm with or without polyinosinic/polycytidylic acid (poly (I:C)) induction. Furthermore, overexpression of ToIRF1 or ToIFNa3 shows that ToIRF1 can notably activate ToIFNa3 and interferon signaling molecule expression. Promoter sequence analysis finds that several interferon stimulating response element (ISRE) binding sites are present in the promoter of ToIFNa3. Additionally, truncation, point mutation, and electrophoretic mobile shift (EMSA) assays confirmed that ToIRF1 M5 ISRE binding sites are functionally important for ToIFNa3 transcription. These results may help to illuminate the roles of teleost IRF1 in the transcriptional mechanisms of type I IFN in the immune process.