bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of key genes and biological pathways in different parts of yak oviduct based on transcriptome analysis
Authors: Zhang Jian , Pan Yangyang , Zhao Ling , Zhao Tian , Yu Sijiu , Cui Yan
Published: November 2022

The oviduct consists of three parts: the infundibulum (In), ampulla (Am), and isthmus (Is). These have the same histological structure, but different physiological functions. In this study, transcriptomics was used to analyze mRNA in these three parts of yak oviduct. The results showed that there were 325 up-regulated genes and 282 down-regulated genes in the infundibulum and ampulla. Moreover, there were 234 up-regulated genes and 776 down-regulated genes in the isthmus and ampulla, as well as 873 up-regulated genes and 297 down-regulated genes in the infundibulum and isthmus. The expression of C3 in the infundibulum was significantly higher than that in the ampulla and isthmus. The expression of FAU in the isthmus was significantly lower than that in the ampulla and infundibulum, and the expression of EEF1A1 in the ampulla was significantly higher than that in the ampulla and infundibulum. When the infundibulum was compared with the ampulla and isthmus, it was found that the up-regulated genes were enriched in the lysosome, phagosome, staphylococcus aureus infection, and leishmaniasis pathway. When the isthmus was compared with the ampulla and infundibulum, the up-regulated genes were present in the apoptosis pathway, oxidative phosphorylation, and viral myocarditis pathway. When the isthmus was compared with the infundibulum and ampulla, the down-regulated pathways were protein processing in the endoplasmic reticulum and the endocytosis. The Epstein–Barr virus infection pathway was up-regulated according to a comparison of the isthmus and infundibulum and was down-regulated based on a comparison of the isthmus and ampulla. Transcriptional misregulation in the Middle East pathway was up-regulated based on a comparison of the isthmus and ampulla and was down-regulated based on a comparison of the isthmus and infundibulum. ERBB2, JUP, CTNND1, and KRT7 were defined as the hub genes of the yak oviduct. The results of this study provide sufficient omics data for yak fertilization, which is also of great significance to altitude medicine.
A Simplified Algorithm for Dealing with Inconsistencies Using the Analytic Hierarchy Process
Authors: Pascoe Sean
Published: November 2022

Inconsistencies in the comparison matrix is a common problem in many studies using the analytic hierarchy process (AHP). While these may be identified and corrected through asking respondents to reconsider their choices, this is not always possible. This is particularly the case for online surveys, where the number of respondents may be large and often anonymous, such that interacting with individual respondents is neither feasible nor possible. Several approaches have previously been developed for autonomously adjusting the comparison matrix to deal with inconsistencies. In this paper, we build on these previous approaches, and present an algorithm that is conceptually and analytically simple and readily implementable in R. The algorithm is applied to several example cases to illustrate its performance, including an example case study involving data collected through a large online survey. The results suggest that the modified survey-derived comparison matrix derived using the algorithm produces consistent responses that do not substantially alter the individual preferences in most cases.
Structural Autonomy and Management Performance: An Influence Reinforced in the Particular Context of Portuguese Public Secondary Schools
Authors: Fontes Carla , Leite Joaquim , Fernandes Paula Odete
Published: November 2022

The purpose of this research is to verify interdependencies among service innovation, structural autonomy, performance measurement systems, and organizational outcomes in a new organizational context. The relationships between these variables that structure organizational design have been studied for several decades, but it is necessary to verify the validity of those interdependencies in new geographical contexts and periods. Contingency theory was the selected framework because it argues how different scenarios can justify different organizational solutions and is appropriate in the predominant quantitative research. For data collection, surveys were sent to 526 executive heads of all Portuguese public secondary schools, obtaining 154 valid responses. Data processing was carried out using descriptive analysis, Spearman correlation, and structural equation analysis. The results reinforce previous literature, proving the influence of structural autonomy, not only on pedagogical management performance but also on resource management performance within a specific context explored for the first time in the Portuguese public sector. However, the interdependence of these variables in relation to pedagogical innovation strategy and organizational outcomes of effectiveness and efficiency was not verified in this particular setting, corroborating the basic foundations of contingency theory.
Is the Rural Population Caught in the Whirlwind of the Digital Divide?
Authors: Kerras Hayet , Rosique Contreras María Francisca , Bautista Susana , de-Miguel Gómez María Dolores
Published: November 2022

Technology represents a benchmark ally for today’s rural world and is a sine qua non-condition for achieving sustainable development. Indeed, today the arrival of digitization and information and communication tools makes life easier for the inhabitants of the rural world in general and for those who work in agriculture. However, not everyone has and knows how to use these technologies. There are very visible differences between the rural world and the urban one in the accessibility and use of technology, especially among vulnerable people (unemployed, elderly, women, etc.), causing a digital divide that reflects the great discrimination suffered by the rural world, full of stereotypes and very traditional role assignments. The objective of this study is to evaluate the differences in terms of access and use of technology. For this reason, the results of a survey carried out on the Spanish rural population have been analyzed with the structural equations tool “PLS-SEM”. They show digital gaps, as well as a disturbance between the different gaps and the socioeconomic situation of users, which imposes the need to take immediate measures to reduce and fight against this type of inequality.
Combined Reproductive Effects of Imidacloprid, Acetochlor and Tebuconazole on Zebrafish (Danio rerio)
Authors: Yang Jin , Chang Yiming , Zhang Yanning , Zhu Lizhen , Mao Liangang , Zhang Lan , Liu Xingang , Jiang Hongyun
Published: November 2022

Pesticides usually occur as mixtures of multiple chemicals in the natural aquatic ecosystem, so research based on the toxicity data of a single compound on aquatic organisms is not enough to accurately assess the actual toxicity risk of pesticides. There is still a gap in the research on the reproductive toxicity of combined insecticides, herbicides and fungicides on zebrafish (Danio rerio). In this study, zebrafish were used to systematically investigate the separate and combined reproductive toxicity of imidacloprid (IMI), acetochlor (ACT) and tebuconazole (TBZ), which are commonly used in rice fields. Adult zebrafish were exposed to the three pesticides individually and in combination for 28 days, and the number, heartbeat, deformation rate, body length, and swim bladder development of F1 offspring embryos were observed and the reproductive hormones testosterone (T), estradiol (E2), and vitellogenin (VTG) contents and the expressions of nine reproductive genes (ar, esr2a, vtg1, gr, star, fshr, hmgcrb, 3βhsd and vasa) in the testes of the male and the ovaries of the female F0 zebrafish adults were measured to evaluate the individual and combined effects. The results showed that exposure to the mixtures of IMI, ACT and TBZ resulted in a decrease in heartbeat, body length and swim bladder development and an increase in the deformity rate of F1 offspring embryos compared to the individual exposure groups. In the combined exposure group, the content of T decreased significantly and the content of VTG increased significantly in the testes of the males; the content of T significantly increased, while the content of E2 and VTG significantly decreased in the ovaries of the females, indicating that combined exposure showed a more obvious endocrine-disrupting effect compared to the individual exposures. In addition, the expression of nine reproductive genes was significantly altered compared to the individual exposure groups. Therefore, our results indicated that the mixture of IMI, ACT and TBZ caused fewer number of F1 embryos, higher developmental defects of F1, greater disruption in the content of reproductive hormones and the expression of reproductive genes compared to the individual pesticides at the corresponding doses. Therefore, the presence of pesticides in mixtures in the real water environment is likely to increase the toxic reproductive effects on zebrafish and cause more serious impacts on aquatic ecosystems.
Microbiota Community Structure and Interaction Networks within Dermacentor silvarum, Ixodes persulcatus, and Haemaphysalis concinna
Authors: Qiu Hongyu , Lv Qingbo , Chang Qiaocheng , Ju Hao , Wu Tingting , Liu Shunshuai , Li Xiuwen , Yan Yimeng , Gao Junfeng , Wang Chunren
Published: November 2022

Ticks carry and transmit a variety of pathogens, which are very harmful to humans and animals. To characterize the microbial interactions in ticks, we analysed the microbiota of the hard ticks, Dermacentor silvarum, Ixodes persulcatus, and Haemaphysalis concinna, using 16S rRNA, showing that microbial interactions are underappreciated in terms of shaping arthropod microbiomes. The results show that the bacterial richness and microbiota structures of these three tick species had significant differences. Interestingly, the bacterial richness (Chao1 index) of all ticks decreased significantly after they became engorged. All the operational taxonomic units (OTUs) were assigned to 26 phyla, 67 classes, 159 orders, 279 families, and 627 genera. Microbial interactions in D. silvarum demonstrated more connections than in I. persulcatus and H. concinna. Bacteria with a high abundance were not important families in microbial interactions. Positive interactions of Bacteroidaceae and F_Solibacteraceae Subgroup 3 with other bacterial families were detected in all nine groups of ticks. This study provides an overview of the microbiota structure and interactions of three tick species and improves our understanding of the role of the microbiota in tick physiology and vector capacity, thus being conducive to providing basic data for the prevention of ticks and tick-borne diseases.
Mitragyna speciosa Korth Leaf Pellet Supplementation on Feed Intake, Nutrient Digestibility, Rumen Fermentation, Microbial Protein Synthesis and Protozoal Population in Thai Native Beef Cattle
Authors: Phesatcha Burarat , Phesatcha Kampanat , Wanapat Metha
Published: November 2022

This experiment evaluated the use of Mitragyna speciosa Korth leaf pellets (MSLP) on feed intake and nutrient digestibility in Thai native beef cattle. Four Thai native beef cattle steers were randomly assigned according to a 4 × 4 Latin square design to receive four dietary treatments. The treatments were as follows: control (no supplementation), MSLP supplement at 10 g/hd/d, MSLP supplement at 20 g/hd/d and MSLP supplement at 30 g/hd/d, respectively. All animals were fed a concentrate mixture at 0.5% body weight, while urea lime-treated rice straws were fed ad libitum. Findings revealed that feed intakes were increased by MSLP, which also significantly increased the digestibility of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF). Ruminal total volatile fatty acid (TVFA) concentration and propionate (C3) proportion were increased (p < 0.05) with MSLP supplementation, whereas ruminal ammonia-N (NH3-N), plasma urea nitrogen (PUN), acetate (C2), C2:C3 ratio and estimated methane (CH4) production decreased (p < 0.05). Total bacterial, Fibrobacter succinogenes and Ruminococus flavefaciens populations increased (p < 0.05) at high levels of MSLP supplementation, while protozoal populations and methanogenic archaea reduced (p < 0.05). Supplementation of MSLP also increased the efficiency of microbial nitrogen protein synthesis. Supplementing beef cattle with MSLP 10–30 g/hd/d significantly increased rumen fermentation end products and nutrient digestibility by mitigating protozoal populations and estimated CH4 production.
Hernioplasty with Peritoneal Flap for the Surgical Treatment of Umbilical Hernia in Swine
Authors: Spadola Filippo , Neve Veronica Cristina , Interlandi Claudia Dina , Spadaro Andrea , Macrì Francesco , Iannelli Nicola Maria , Costa Giovanna Lucrezia
Published: November 2022

Background: Umbilical hernia is one of the most common developmental defects in swine, producing large economic losses for farmers, forced to slaughter animals at a younger age and therefore at a lower weight to prevent fatal complications. This study describes a surgical technique to repair umbilical hernia through the use of autologous prostheses, allowing recovery of the affected animals; Methods: After a general examination of the swine and examination of the lesions, the swine were anesthetized and underwent surgery. The surgery was performed by combining the traditional herniorrhaphy with the inclusion and fixation of a peritoneal flap obtained from the incision of the same hernial sac; Results: Follow-ups were carried out at 7, 30 and 60 days and demonstrated healing in all of the treated subjects; Conclusions: The use of this surgical technique allows for providing resistance to herniorrhaphy performed through the use of a cost-free autologous biomaterial prosthesis, with excellent tissue compatibility. This might allow for reducing significantly the rate of relapses and eliminating the risk of rejection.
Dietary Effect of Palm Kernel Oil Inclusion in Feeding Finishing Lambs on Meat Quality
Authors: Castro Daniela Pionorio Vilaronga , Pimentel Paulo Roberto Silveira , dos Santos Neiri Jean Alves , da Silva Júnior Jarbas Miguel , Virginio Júnior Gercino Ferreira , de Andrade Ederson Américo , Barbosa Analívia Martins , Pereira Elzânia Sales , Ribeiro Cláudio Vaz Di Mambro , Bezerra Leilson Rocha , Oliveira Ronaldo Lopes
Published: November 2022

This study evaluated the effects of palm kernel oil (PKO) in the diet of lambs on carcass characteristics, quality, and fatty acid profile of the meat. Forty uncastrated male Santa Inês lambs were used and divided among the treatments: PKOzero without inclusion; PKO1.3—added 1.3%; PKO2.6—added 2.6%; PKO3.9—added 3.9%; PKO5.2—added 5.2%. The carcass characteristics, the variables related to meat color, and the chemical composition of the Longissimus lumborum of lambs were not affected by the PKO inclusion. The weight of the carcasses at slaughter, hot and cold, half carcass, loin-eye area, and commercial cuts decreased linearly when PKO was added to the lamb diet (p < 0.01). CCY decreased linearly to the inclusion level of 2.66% PKO (RMSE 2.204). Total conjugated linoleic acid CLA and C18:3 n-3 GA concentrations remained stable until the inclusion levels of 3.44% PKO (RMSE 0.0956) and 2.17% (RMSE 0.0637), decreasing its concentrations as the increased level of PKO. The presence of PKO in the lambs’ diet up to the level of 5.2% did not change the meat quality characteristics; thus, from the point of view of lamb meat production and fatty acid profile, the inclusion of PKO is not beneficial.
Sunflower Cake from the Biodiesel Industry in the Diet Improves the Performance and Carcass Traits of Nellore Young Bulls
Authors: da Silva Oliveira Vinicius , Barbosa Analivia M. , de Andrade Ederson A. , Virginio Júnior Gercino F. , Nascimento Thiago V. C. , Lima Anny Graycy Vasconcelos de Oliveira , Portela Ricardo W. D. , da Silva Júnior Jarbas M. , Pereira Elzânia S. , Bezerra Leilson R. , Oliveira Ronaldo L.
Published: November 2022

We aimed to determine the optimal inclusion level of sunflower cake (0, 90, 180, and 270 g/kg total DM) as a partial replacement of soybean meal and corn ground in young bulls’ diets by examining nutrient intake and digestibility, ingestive behavior, nitrogen balance, metabolic serum profile, growth performance, and carcass traits. Thirty-two intact Nellore bulls (BW 374 ± 42.5) were distributed in a completely randomized design. The experiment lasted 90 days. The final BW of the animals was 515.25 ± 24.7. There was a linear decrease effect in the intake of DM, crude protein and nonfibrous carbohydrates, eating and rumination efficiency, N-urinary, N-total excretion, and blood urea nitrogen. Sunflower cake did not affect the NDF digestibility, nitrogen (N)-fecal excretion, blood metabolites, Longissimus lumborum muscle area, or subcutaneous fat deposition. There were linear and quadratic effects on the eating and rumination time, microbial protein production and efficiency, gamma-glutamyl transferase and cholesterol serum concentrations, and muscle carcass tissue. There was a quadratic effect on ether extract intake, final BW, and total gain with the inclusion of sunflower cake in the young bull’s diet. The replacement of soybean meal and corn ground with sunflower cake at the level of 90 g/kg of DM in the diet of young bulls is recommended because it reduces the DM intake and digestibility, increases microbial protein synthesis and muscle tissue deposition, and consequently improves the performance, feed efficiency, and carcass traits.
Comparative Efficacy in Challenge Dose Models of a Toxin Expressing Whole-Cell Vaccine against Eight Serovars of Actinobacillus pleuropneumoniae in Pigs
Authors: Mortensen Preben , Toft Nils , Kiss István , Palya Vilmos , Smits Han , Tenk Miklós
Published: November 2022

Actinobacillus pleuropneumoniae is a major economically significant bacterial respiratory pig pathogen, and whole cell vaccines are used to prevent disease. However, there is little data available on multi-serovar whole cell vaccine protection. Therefore, we determined the protective efficacies of a whole-cell A. pleuropneumoniae serovar 1 and 2 vaccine comprising ApxI-III toxins (C-vaccine, Coglapix®, Ceva, France) against serovars 1, 2, 4, 5, 6, 7, 9/11, and 13. The infection doses used induced disease representative of endemic field conditions, and standard protocols were used for all studies. Protection against homologous serovars 1 and 2 significantly reduced lung lesion scores (LLS) compared to positive controls: p = 0.00007 and p = 0.00124, respectively. The protection against heterologous serovars 4, 5, 6, 7, 9/11, and 13 also significantly reduced LLS: range p = 2.9 × 10−10 to p = 0.00953. As adjudged by the estimated random effect, reproducibility between studies was high. A highly significant serovar-independent reduction of pathological lung lesions by the C-vaccine was found for all the serovars tested (1, 2, 4, 5, 6, 7, 9/11, and 13). We conclude that the C-vaccine gives high serovar-independent protection against disease and is suitable for this use in the field.
Milk Pathogens in Correlation with Inflammatory, Oxidative and Nitrosative Stress Markers in Goat Subclinical Mastitis
Authors: Novac Cristiana Ștefania , Nadăș George Cosmin , Matei Ioana Adriana , Bouari Cosmina Maria , Kalmár Zsuzsa , Crăciun Smaranda , Fiț Nicodim Iosif , Dan Sorin Daniel , Andrei Sanda
Published: November 2022

Goat mastitis is still frequently diagnosed in dairy farms, with serious consequences on milk quality and composition. The aim of this study was to establish correlations between milk microorganisms and biochemical parameters in goats with no signs of clinical mastitis. Thus, 76 milk samples were collected from a dairy goat farm, Carpathian breed, followed by microbiological, molecular (16S rRNA sequencing) and somatic cells analysis, determination of lactate dehydrogenase (LDH), β-glucuronidase, catalase (CAT), glutathione peroxidase (GPx) activity, total antioxidant capacity (TAC), nitric oxide (NO) and lipid peroxides (LPO) using spectrophotometry and the ELISA method for 8-hydroxy-deoxyguanosine (8-OHdG) as the oxidative DNA damage indicator. Samples positive for bacterial growth showed a significant (p < 0.05) increase in the number of somatic cells, LDH and β-glucuronidase activity, as well as higher levels of CAT, GPx, NO, LPO and 8-OHdG compared with pathogen-free milk whereas TAC was lower in milk from an infected udder. These findings suggest that subclinical mastitis is associated with increased enzymatic activity and induction of oxidative stress. Nevertheless, changes in biochemical parameters tended to vary depending on the pathogen, the most notable mean values being observed overall in milk positive for Staphylococcus aureus.
Body Condition and Breeding of Urban Red Squirrels: Comparison of Two Populations Affected by Different Levels of Urbanization
Authors: Beliniak Agata , Gryz Jakub , Klich Daniel , Jasińska Karolina , Krauze-Gryz Dagny
Published: November 2022

The red squirrel is among the mammals that have adjusted well to urban habitats. Here, we focused on the two populations inhabiting Warsaw: in a park (with year-round supplemental feeding) and in an urban forest. We hypothesised that park squirrels would have higher body mass (and better body condition), being more stable over the year, and would have a higher breeding rate (i.e., the share of breeding females). Contrary to our hypothesis, forest squirrels were heavier and had better body condition than park squirrels. The body masses of squirrels from both areas were quite stable (with the highest values obtained in spring). Females in better body conditions were more likely to breed. More breeding females and sub-adults were trapped in the park. Regardless of the study site, the highest share of breeding females was in spring, but they also bred in winter and in the remaining seasons. The lower body mass/condition of park squirrels may be possibly explained by high intraspecific competition, or by stable food (and thermal as typical for the city) conditions, in which accumulating fat was not crucial. Mild winter conditions may have also enabled squirrels to breed early. This study showed the high plasticity of red squirrels living in human-transformed habitats.
Expectations versus Reality of Designer Dog Ownership in the United States
Authors: Hladky-Krage Bridget , Hoffman Christy L.
Published: November 2022

“Designer dogs”, which are the hybrid offspring that result from intentionally breeding dogs belonging to different breeds, are an extremely popular pet choice in the United States. Poodle mixes, often called “doodles”, are a very common type of designer dog. However, there are many misconceptions surrounding them, and the reality of owning one may not match the owner’s expectations. For instance, many people believe these dogs to be non-shedding and hypoallergenic, although this is not always the case. This study explored whether the reality of owning a doodle matches owner expectations. For comparison purposes, we also asked owners of non-doodle dogs about their expectations versus reality. Our survey-based study included 2191 owners of doodles and non-doodle dogs recruited via groups of dog owners on Facebook and Reddit. The data showed that, when selecting their dogs, doodle owners were more influenced than non-doodle owners by their dog’s appearance and by the perception that doodles are good with children and are generally healthy. Doodle owners reported being highly satisfied with their dogs; nevertheless, more than twice as many doodle owners than owners of the other groups of dogs reported that their dog’s maintenance requirements, such as their need for regular grooming, were more intensive than they had expected. This finding suggests that those interested in owning doodles would benefit from having more information about their dog’s grooming needs so they can decide whether they have the time and money required to meet their dog’s welfare needs.
Air Quality and Residents’ Health in China: An Empirical Analysis Based on Spatial Simultaneous Equations
Authors: He Hua , Li Zining
Published: November 2022

Large-scale air pollution has an impact on the health and travel of residents in China. First, this article uses the gravity model and finds that China’s air quality presents a typical spatial correlation network. The network structure has changed from complex to simple, indicating that China’s air quality is gradually improving. Second, this paper uses spatial simultaneous equations to study the effect of air quality on residents’ health. The results show that an increase in the air quality index of local and surrounding provinces will have a significant impact on the health of residents. The absolute and relative air quality affects the lives of residents. The decline in the health of residents from the previous period will prompt the government to adopt various pollution reduction policies, which will improve air quality in the current period. Based on the empirical research conclusions, this article makes policy recommendations.
Assessing the Impact of Natural Conditions/Socioeconomic Indicators on the Urban Thermal Environment Based on Geographic Big Data
Authors: Lu Xiaolong , Wang Haihui , Chen Huanliang , Gao Shuai
Published: November 2022

Understanding correctly the factors influencing the urban thermal environment is a prerequisite and basis for formulating heat-island-effect mitigation policies and studying urban ecological issues. The rapid urbanization process has led to the gradual replacement of natural landscapes by products of socioeconomic activities, and although previous studies have shown that natural conditions and socioeconomic intensity can significantly influence land surface temperature (LST), few studies have explored the combined effects of both on LST, especially at a fine scale. Therefore, this study investigated the relationship between natural conditions/socioeconomic and summer daytime LST based on big data and a random forest (RF) algorithm using the city of Jinan as the study area. The results showed that the spatial pattern of LST, natural condition characteristics of the city, and socioeconomic characteristics are consistent in spatial pattern and have significant correlation. In the RF model, the fitted R2 of the regression model considering two influencing factors reaches 0.86, which is significantly higher than that of the regression model considering only one influencing factor. In the optimal regression model, topographic factors in natural conditions and socioeconomic factors in buildings and roads are very important factors influencing the urban thermal environment. Based on the results, strategies and measures for developing and managing measures related to the thermal environment are discussed in depth. The results can be used as a reference for mitigating urban heat islands in the study area or other cities with similar characteristics.
Forecasting and Optimization of Wind Speed over the Gobi Grassland Wind Farm in Western Inner Mongolia
Authors: Xin Jinyuan , Bao Daen , Ma Yining , Ma Yongjing , Gong Chongshui , Qiao Shuai , Jiang Yunyan , Ren Xinbing , Pang Tao , Yan Pengcheng
Published: November 2022

Wind power, as one of the primary clean energies, is an important way to achieve the goals of carbon peak and carbon neutrality. Therefore, high-resolution measurement and accurate forecasting of wind speed are very important in the organization and dispatching of the wind farm. In this study, several methodologies, including the mesoscale WRF (Weather Research and Forecasting(WRF) model, mathematical statistics algorithms, and machine learning algorithms, were adopted to systematically explore the predictability and optimization of wind speed of a Gobi grassland wind farm located in western Inner Mongolia. Results show that the rear-row turbines were significantly affected by upwind turbine wakes. The output power of upwind-group turbines was 591 KW with an average wind speed of 7.66 m/s, followed by 532 KW and 7.02 m/s in the middle group and 519 KW and 6.92 m/s in the downwind group. The higher the wind speed was, the more significantly the wake effect was presented. Intercomparison between observations and WRF simulations showed an average deviation of 3.73 m/s. Two postprocessing methods of bilinear interpolation and nearest replacement could effectively reduce the errors by 34.85% and 36.19%, respectively, with average deviations of 2.43 m/s and 2.38 m/s. A cycle correction algorithm named Average Variance–Trend (AVT) can further optimize the errors to 2.14 m/s and 2.13 m/s. In another aspect, the categorical boosting (CatBoost) artificial intelligence algorithm also showed a great performance in improving the accuracy of WRF outputs, and the four-day average deviation of 26–29 September decreased from 3.21 m/s to around 2.50 m/s. However, because of the influence of large-scale circulations, there still exist large errors in the results of various correction algorithms. It is therefore suggested through the investigation that data assimilation of the northwest and Mongolian plateau, boundary layer parameterization scheme optimization, and embedding of high-resolution topographic data could have great potential for obtaining more accurate forecasting products.
Using a Pollution-to-Risk Method to Evaluate the Impact of a Cold Front: A Case Study in a Downstream Region in Southeastern China
Authors: Lin Changqing , Zhang Wengwan
Published: November 2022

Cold fronts frequently intrude China in winter, causing air pollution episodes in downwind regions. Fine particulate matter (PM2.5) has been used as a major proxy of air pollution to examine the impacts of cold fronts. Compared to particles, gaseous pollutants can cause comparable or even higher levels of short-term health risks. In this study, a pollution-to-risk model was used to systematically evaluate the impacts of cold fronts on the combined health risks of air pollution mixtures, including PM2.5, nitrogen dioxide (NO2), ozone (O3), and sulfur dioxide (SO2). Dominant pollutants that caused significant mortality risks during a cold frontal passage in December 2019 over Fuzhou, a downwind city in southeastern China, were then examined. Under northerly frontal airflows, a pollution belt propagated southwards. In Fuzhou, two pollution peaks existed during the cold frontal passage. At the first peak, convergence and stagnant air in the frontal zone rapidly accumulated local air pollutants. The dominant pollutants that caused the mortality risk were identified as NO2 and PM2.5, both of which contributed 45% to the total risk. At the second peak, advection transported a significant amount of secondary pollutants from the upwind regions. Although PM2.5 was the dominant pollutant at this peak, gaseous pollutants still accounted for 34% of the total risk. Our risk analyses underscore the significant health impacts of gaseous pollutants during cold frontal passages in winter. The results generated from this study will help guide environmental policy makers in forming and improving air pollution control strategies during pollution episodes.
The Extremely Active 2020 Hurricane Season in the North Atlantic and Its Relation to Climate Variability and Change
Authors: Hernández Ayala José Javier , Méndez-Tejeda Rafael
Published: November 2022

The 2020 hurricane season in the North Atlantic basin was the most active on record, with 30 named tropical cyclones. In this study, climate trends in oceanic and atmospheric parameters (including the sea surface temperatures, ocean heat content, cloud cover, mid-level humidity, vertical wind shear, and sea level pressure) were used to model the tropical cyclone, hurricane, and major hurricane frequency in the post-satellite era (1966–2020). The relationships between storm frequency and climate variability factors (including the El Niño Southern Oscillation, the North Atlantic Oscillation, the Atlantic Multidecadal Oscillation, and the Atlantic Meridional Mode) were also examined. This was performed to determine the factors that exerted the greatest influence on the most active hurricane seasons on record. Mann–Kendall trend tests, Pearson’s correlations tests, stepwise Poisson linear regression models and spatial analysis techniques were used to identify the climate change and variability factors that best explained the tropical cyclone frequency in the North Atlantic. Our results show that hyperactive hurricane seasons, such as that of 2020, tend to be associated with higher cloud cover development, lower sea level pressure patterns, higher sea surface temperatures, positive phases of the Atlantic Multidecadal Oscillation and the Atlantic Meridional Mode, and weaker wind shear environments. Seasons with more major hurricanes had higher ocean heat contents and weaker wind shear environments. The 2020 and 2005 seasons had similar cloud cover and sea level pressure patterns, yet the wind shear was lower in 2020 than in 2005, which was associated with La Niña dominant conditions that could explain why 2020 surpassed 2005 in the total number of storms.
Mitigating Polysulfide Shuttles with Upcycled Alkali Metal Terephthalate Decorated Separators
Authors: Gribble Daniel A. , Lin Zih-Yu , Ghosh Sourav , Savoie Brett M. , Pol Vilas G.
Published: November 2022

High energy density lithium–sulfur batteries (LSBs) are a potential replacement for lithium-ion batteries (LIBs). However, practical lifetimes are inhibited by lithium polysulfide (LiPS) shuttling. Concurrently, plastic waste accumulation worldwide threatens our ecosystems. Herein, a fast and facile strategy to upcycle polyethylene terephthalate (PET) waste into useful materials is investigated. Dilithium terephthalate (Li2TP) and dipotassium terephthalate (K2TP) salts were synthesized from waste soda bottles via microwave depolymerization and solution coated onto glass fiber paper (GFP) separators. Salt-functionalized separators with Li2TP@GFP and K2TP@GFP mitigated LiPS shuttling and improved electrochemical performance in cells. Pore analysis and density functional theory (DFT) calculations indicate the action mechanism is synergistic physical blocking of bulky LiPS anions in nanopores and diffusion inhibition via electrostatic interactions with abundant carboxylate groups. LSBs with K2TP@GFP separator showing highest LiPS affinity and smallest pore size demonstrated enhanced initial capacity as compared to non-modified GFP by 5.4% to 648 mAh g−1, and increased cycle 100 capacity by 23% to 551 mAh g−1. Overall, K2TP@GFP retained 85% of initial capacity after 100 cycles with an average capacity fading of 0.15% per cycle. By comparison, GFP retained only 73% of initial capacity after 100 cycles with 0.27% average capacity loss, demonstrating effective LiPS retention.
Plasma Metabolic Disturbances in Parkinson’s Disease Patients
Authors: Gątarek Paulina , Sekulska-Nalewajko Joanna , Bobrowska-Korczaka Barbara , Pawełczyk Małgorzata , Jastrzębski Karol , Głąbiński Andrzej , Kałużna-Czaplińska Joanna
Published: November 2022

Plasma from patients with Parkinson’s disease (PD) is a valuable source of information indicating altered metabolites associated with the risk or progression of the disease. Neurotoxicity of dopaminergic neurons, which is triggered by aggregation of α-synuclein, is the main pathogenic feature of PD. However, a growing body of scientific reports indicates that metabolic changes may precede and directly contribute to neurodegeneration. Identification and characterization of the abnormal metabolic pattern in patients’ plasma are therefore crucial for the search for potential PD biomarkers. The aims of the present study were (1) to identify metabolic alterations in plasma metabolome in subjects with PD as compared with the controls; (2) to find new potential markers, some correlations among them; (3) to identify metabolic pathways relevant to the pathophysiology of PD. Plasma samples from patients with PD (n = 25) and control group (n = 12) were collected and the gas chromatography-time-of-flight-mass spectrometry GC-TOFMS-based metabolomics approach was used to evaluate the metabolic changes based on the identified 14 metabolites with significantly altered levels using univariate and multivariate statistical analysis. The panel, including 6 metabolites (L-3-methoxytyrosine, aconitic acid, L-methionine, 13-docosenamide, hippuric acid, 9,12-octadecadienoic acid), was identified to discriminate PD from controls with the area under the curve (AUC) of 0.975, with an accuracy of 92%. We also used statistical criteria to identify the significantly altered level of metabolites. The metabolic pathways involved were associated with linoleic acid metabolism, mitochondrial electron transport chain, glycerolipid metabolism, and bile acid biosynthesis. These abnormal metabolic changes in the plasma of patients with PD were mainly related to the amino acid metabolism, TCA cycle metabolism, and mitochondrial function.
Effect of High-Fructose Diet-Induced Metabolic Syndrome on the Pituitary-Gonadal Axis in Male Rats
Authors: Hsia Shih-Min , Chiang Yi-Fen , Chen Hsin-Yuan , Ali Mohamed , Wang Paulus S. , Wang Kai-Lee
Published: November 2022

Plasma testosterone levels have been found to decrease in older insulin-resistant male patients. Both lower total testosterone levels and a higher incidence of metabolic syndrome have also been reported. The aim of this study was to investigate the effects of high-fructose diet-induced diabetes on both the testosterone release by Leydig cells and the activity of the hypothalamus–pituitary–gonadal (HPG) axis in male rats. Male rats were fed with either standard chow (control group) or a high-fructose diet (fructose-fed group) for 21 weeks. Hyperglycemia, hyperinsulinemia, and hypertension were observed in the fructose-fed group. Moreover, plasma testosterone and LH levels decreased in the fructose-fed group compared to the control group. Sperm motility was also reduced by 15% in the fructose-fed rats. In contrast, the basal release of testosterone from rat Leydig cells was not altered by fructose feeding. Moreover, in vitro studies showed that the testosterone release, in response to different stimulants, including forskolin (an adenylyl cyclase activator, 10−5 M), 8-Br-cAMP (a permeable analog of cAMP, 10−5 M), A23187 (a calcium ionophore, 10−5 M), or 25-hydroxy-cholesterol (water-soluble cholesterol, 10−5 M), did not significantly differ between the fructose-fed and control groups. Interestingly, the release of testosterone in response to human chorionic gonadotropin (hCG, 0.05 IU/mL) was enhanced by eightfold in the control group, but elevenfold in the fructose-fed group. LH receptor expression in rat Leydig cells was also increased. Moreover, LH secretion from the anterior pituitary was altered in the fructose diet-fed group. These results suggest that fructose diet-fed rats have lower plasma testosterone levels, which can lead to a higher sensitivity of hCG in Leydig cells.
CircDOCK1 Regulates miR-186/DNMT3A to Promote Osteosarcoma Progression
Authors: Jin Zhihui , Ye Jia , Chen Sen , Ren Yijun , Guo Weichun
Published: November 2022

Background: Circular RNAs (circRNAs), as a class of endogenous RNAs, are implicated in osteosarcoma (OS) progression. However, the functional properties of circDOCK1 in OS have been largely unexplored. The present study demonstrated the regulatory mechanism of circDOCK1 in OS. Methods: QRT-PCR and Western blots were used to determine the abundances of circDOCK1, miR-186, and DNMT3A. Cell counting kit-8 (CCK-8), 5-Ethynyl-2′-deoxyuridine (EdU), colony formation, Transwell, and wound healing assays were used to examine cellular multiplication, motility, and invasion. Luciferase reporter analysis, RNA immunoprecipitation (RIP), and pull-down assays were used to verify target relationships. Xenograft models were used to analyze in vivo function. Results: OS tissues and cells showed high levels of circDOCK1. By knocking down circDOCK1, cellular multiplication, motility, and invasion were suppressed. Furthermore, silencing circDOCK1 suppressed the growth of tumor xenografts. According to mechanistic studies, miR-186 targets DNA methyltransferases 3A (DNMT3A) directly and acts as a circDOCK1 target. Furthermore, circDOCK1 upregulated DNMT3A expression through sponging miR-186 to regulate the progression of OS. Conclusions: CircDOCK1 promotes OS progression by interacting with miR-186/DNMT3ADNMT3A, representing a novel therapeutic approach.
Do Semaphorins Play a Role in Development of Fibrosis in Patients with Nonalcoholic Fatty Liver Disease?
Authors: Šamadan Lara , Papić Neven , Mijić Maja , Knežević Štromar Ivana , Gašparov Slavko , Vince Adriana
Published: November 2022

Nonalcoholic fatty liver disease (NAFLD) is associated with systemic changes in immune response linked with chronic low-grade inflammation and disease progression. Semaphorins, a large family of biological response modifiers, were recently recognized as one of the key regulators of immune responses, possibly also associated with chronic liver diseases. The aim of this study was to identify semaphorins associated with NAFLD and their relationship with steatosis and fibrosis stages. In this prospective, case-control study, serum semaphorin concentrations (SEMA3A, -3C, -4A, -4D, -5A and -7A) were measured in 95 NAFLD patients and 35 healthy controls. Significantly higher concentrations of SEMA3A, -3C and -4D and lower concentrations of SEAMA5A and -7A were found in NAFLD. While there was no difference according to steatosis grades, SEMA3C and SEMA4D significantly increased and SEMA3A significantly decreased with fibrosis stages and had better accuracy in predicting fibrosis compared to the FIB-4 score. Immunohistochemistry confirmed higher expression of SEMA4D in hepatocytes, endothelial cells and lymphocytes in NAFLD livers. The SEMA5A rs1319222 TT genotype was more frequent in the NAFLD group and was associated with higher liver stiffness measurements. In conclusion, we provide the first evidence of the association of semaphorins with fibrosis in patients with NAFLD.
Polyindole-Derived Nitrogen-Doped Graphene Quantum Dots-Based Electrochemical Sensor for Dopamine Detection
Authors: Thadathil Anjitha , Thacharakkal Dipin , Ismail Yahya A. , Periyat Pradeepan
Published: November 2022

The sensitive monitoring of dopamine levels in the human body is of utmost importance since its abnormal levels can cause a variety of medical and behavioral problems. In this regard, we report the synthesis of nitrogen-doped graphene quantum dots (N-GQDs) from polyindole (PIN) via a facile single-step hydrothermal synthetic strategy that can act as an efficient electrochemical catalyst for the detection of dopamine (DA). The average diameter of N-GQDs was ∼5.2 nm and showed a C/N atomic ratio of ∼2.75%. These N-GQDs exhibit a cyan fluorescence color under irradiation from a 365 nm lamp, while PIN has no characteristic PL. The presence of richly N-doped graphitic lattices in the N-GQDs possibly accounts for the improved catalytic activity of N-GQDs/GCE towards electrocatalytic DA detection. Under optimum conditions, this novel N-GQDs-modified electrode exhibits superior selectivity and sensitivity. Moreover, it could detect as low as 0.15 nM of DA with a linear range of 0.001–1000 µM. In addition, the outstanding sensing attributes of the detector were extended to the real samples as well. Overall, our findings evidence that N-GQDs-based DA electrochemical sensors can be synthesized from PIN precursor and could act as promising EC sensors in medical diagnostic applications.
Fabrication of a Molecularly Imprinted Nano-Interface-Based Electrochemical Biosensor for the Detection of CagA Virulence Factors of H. pylori
Authors: Saxena Kirti , Murti Bayu Tri , Yang Po-Kang , Malhotra Bansi Dhar , Chauhan Nidhi , Jain Utkarsh
Published: November 2022

H. pylori is responsible for several stomach-related diseases including gastric cancer. The main virulence factor responsible for its establishment in human gastric cells is known as CagA. Therefore, in this study, we have fabricated a highly sensitive MIP-based electrochemical biosensor for the detection of CagA. For this, an rGO and gold-coated, screen-printed electrode sensing platform was designed to provide a surface for the immobilization of a CagA-specific, molecularly imprinted polymer; then it was characterized electrochemically. Interestingly, molecular dynamics simulations were studied to optimize the MIP prepolymerization system, resulting in a well-matched, optimized molar ratio within the experiment. A low binding energy upon template removal indicates the capability of MIP to recognize the CagA antigen through a strong binding affinity. Under the optimized electrochemical experimental conditions, the fabricated CagA-MIP/Au/rGO@SPE sensor exhibited high sensitivity (0.275 µA ng−1 mL−1) and a very low limit of detection (0.05 ng mL−1) in a linear range of 0.05–50 ng mL−1. The influence of other possible interferents in analytical response has also been observed with the successful determination of the CagA antigen.
Aptamer-Based Gold Nanoparticles–PDMS Composite Stamps as a Platform for Micro-Contact Printing
Authors: Abbasi Amna Didar , Hussain Zakir , Yang Kun-Lin
Published: November 2022

In the present study, a functional template made up of in situ synthesised gold nanoparticles (AuNPs) is prepared on polydimethylsiloxane (PDMS) for patterning of target protein onto the desired solid substrates. Unlike previous studies in which bioreceptor probes are randomly attached to the PDMS stamp through electrostatic interactions, herein, we propose an AuNPs–PDMS stamp, which provides a surface for the attachment of thiol-modified biorecognition probes to link to the stamp surface through a dative bond with a single anchoring point based on thiol chemistry. By using this platform, we have developed the ability for microcontact printing (µCP) to selectively capture and transfer target protein onto solid surfaces for detection purposes. After µCP, we also investigated whether liquid crystals (LCs) could be used as a label-free approach for identifying transfer protein. Our reported approach provides promise for biosensing of various analytes.
Flexible Wearable Sensors in Medical Monitoring
Authors: Yuan Yingying , Liu Bo , Li Hui , Li Mo , Song Yingqiu , Wang Runze , Wang Tianlu , Zhang Hangyu
Published: November 2022

The popularity of health concepts and the wave of digitalization have driven the innovation of sensors in the medical field. Such continual development has made sensors progress in the direction of safety, flexibility, and intelligence for continuous monitoring of vital signs, which holds considerable promise for changing the way humans live and even treat diseases. To this end, flexible wearable devices with high performance, such as high sensitivity, high stability, and excellent biodegradability, have attracted strong interest from scientists. Herein, a review of flexible wearable sensors for temperature, heart rate, human motion, respiratory rate, glucose, and pH is highlighted. In addition, engineering issues are also presented, focusing on material selection, sensor fabrication, and power supply. Finally, potential challenges facing current technology and future directions of wearable sensors are also discussed.