bluuebunny/crossref_metadata_2025 huggingface dataset:
Phylogenetic study of the genus Pohlia (Mielichhoferiaceae, Bryophyta) based on chloroplast DNA sequences
Authors: OHGUE TAKAYUKI , AKIYAMA HIROYUKI , SUZUKI-AZUMA HIROSHI , NAGAMASU HIDETOSHI
Published: December 2021

The phylogenetic circumscription and taxonomic status of the genus Pohlia in the Mniaceae sensu lato was investigated based on chloroplast DNA sequences (rbcL, rps4, and trnL-F), with a focus on species occurring in Japan. The maximum likelihood and Bayesian inference analyses of sequences obtained from 34 species of Mniaceae s.l., including 13 Pohlia species, suggested that the genus Pohlia and the family Mielichhoferiaceae are not monophyletic in their present circumscription, but confirmed that the family Mniaceae is monophyletic in its traditional sense. These results are congruent with previous molecular phylogenetic studies. Three distinct clades were recognized in the Mielichhoferiaceae, almost corresponding to three sections of Pohlia (Pohlia, Cacodon and Apalodictyon). One of them branched off first within the Mniaceae s.l., and the other two were sister to the remainder of the Mniaceae s.l. The single included Schizymenium formed a monophyletic group with Pohlia sect. Pohlia and Epipterygium with Pohlia sect. Apalodictyon, confirming the results of previous studies. The results indicate that the taxonomic status of the genus Pohlia and family Mielichhoferiaceae are in need of revision. Phylogenetic analyses nested the accessions of P. camptotrachela within P. annotina and P. flexuosa clades, highlighting the need for taxonomic revision of Japanese propaguliferous Pohlia species.
Comprehensive Radar Data for the Contiguous United States: Multi-Year Reanalysis of Remotely Sensed Storms
Authors: Williams Skylar S. , Ortega Kiel L. , Smith Travis M. , Reinhart Anthony E.
Published: December 2021

The Multi-Year Reanalysis of Remotely Sensed Storms (MYRORSS) dataset blends radar data from the WSR-88D network and Near-Storm Environmental (NSE) model analyses using the Multi-Radar Multi-Sensor (MRMS) framework. The MYRORSS dataset uses the WSR-88D archive starting in 1998–2011, processing all valid single-radar volumes to produce a seamless three-dimensional reflectivity volume over the entire contiguous United States with an approximate 5-min update frequency. The three-dimensional grid has an approximate 1 km × 1 km horizontal dimension and is on a stretched vertical grid that extends to 20 km MSL with a maximal vertical spacing of 1 km. Several reflectivity-derived, severe-storm-related products are also produced, which leverage the ability to merge the MRMS and NSE data. Two Doppler velocity-derived azimuthal shear layer maximum products are produced at a higher horizontal resolution of approximately 0.5 km × 0.5 km. The initial period of record for the dataset is 1998–2011. The dataset underwent intensive manual quality control to ensure that all available and valid data were included while excluding highly problematic radar volumes that were a negligible percentage of the overall dataset, but which caused large data errors in some cases. This dataset has applications toward radar-based climatologies, postevent analysis, machine learning applications, model verification, and warning improvements. Details of the manual quality control process are included and examples of some of these applications are presented.
Using Ensemble Data Assimilation to Explore the Environmental Controls on the Initiation and Predictability of Moist Convection
Authors: Minamide Masashi , Posselt Derek J.
Published: December 2021

Atmospheric deep moist convection has emerged as one of the most challenging topics for numerical weather prediction, due to its chaotic process of development and multiscale physical interactions. This study examines the dynamics and predictability of a weakly organized linear convective system using convection permitting EnKF analysis and forecasts with assimilating all-sky satellite radiances from a water vapor sensitive band of the Advanced Baseline Imager on GOES-16. The case chosen occurred over the Gulf of Mexico on 11 June 2017 during the NASA Convective Processes Experiment (CPEX) field campaign. Analysis of the water vapor and dynamic ensemble covariance structures revealed that meso-α-scale (2000–200 km) and meso-β-scale (200–20 km) initial features helped to constrain the general location of convection with a few hours of lead time, contributing to enhancing convective activity, but meso-γ-scale (20–2 km) or even-smaller-scale features with less than 30-min lead time were identified to be essential for capturing individual convective storms. The impacts of meso-α-scale initial features on the prediction of particular individual convective cells were found to be classified into two regimes; in a relatively dry regime, the meso-α-scale environment needs to be moist enough to support the development of the convection of interest, but in a relatively wet regime, a drier meso-α-scale environment is preferable to suppress the surrounding convective activity. This study highlights the importance of high-resolution initialization of moisture fields for the prediction of a quasi-linear tropical convective system, as well as demonstrating the accuracy that may be necessary to predict convection exactly when and where it occurs.
The Influence of Shallow Cloud Populations on Transitions to Deep Convection in the Amazon
Authors: Barber Katelyn A. , Burleyson Casey D. , Feng Zhe , Hagos Samson M.
Published: December 2021

In this study, a pair of convection-permitting (2-km grid spacing), month-long, wet-season Weather Research and Forecasting (WRF) Model simulations with and without the eddy-diffusivity mass-flux (EDMF) scheme are performed for a portion of the Green Ocean Amazon (GoAmazon) 2014/15 field campaign period. EDMF produces an ensemble of subgrid-scale convective plumes that evolve in response to the boundary layer meteorological conditions and can develop into shallow clouds. The objective of this study is to determine how different treatments of shallow cumulus clouds (i.e., with and without EDMF) impact the total cloud population and precipitation across the Amazonian rain forest, with emphasis on impacts on the likelihood of shallow-to-deep convection transitions. Results indicate that the large-scale synoptic conditions in the EDMF and control simulations are nearly identical; however, on the local scale their rainfall patterns diverge drastically and the biases decrease in EDMF. The EDMF scheme significantly increases the frequency of shallow clouds, but the frequencies of deep clouds are similar between the simulations. Deep convective clouds are tracked using a cloud-tracking algorithm to examine the impact of shallow cumulus on the surrounding ambient environment where deep convective clouds initiate. Results suggest that a rapid increase of low-level cloudiness acts to cool and moisten the low to midtroposphere during the day, favoring the transition to deep convection.
Using Social Network Analysis to Assess Climate Change Professionals’ Communications in Iran
Authors: Oji Ruhollah , Hesam Mehdi , Keener Victoria W.
Published: December 2021

Increased cooperation of an interdisciplinary group of climate change professionals as a social network can play a crucial role in adaptation to climate change. To investigate this relationship at the country scale, this study uses a case study in Iran to 1) measure the cooperative relationship among climate change professionals using the network analysis approach and 2) analyze the potential of the network in promoting adaptation measures based on sustainable development. Social network analysis, which is both a quantitative and qualitative method of grounded theory, was used to analyze the data. Data collection was performed using two questionnaires, including network analysis and a survey, as well as a number of semistructured interviews with the climate change professionals. The data were collected from climate change professionals, including a sample of 55 individuals who were surveyed as a cross section of representative participants from a variety of sectors and organizations. The network relationship results have been analyzed using different tests at three levels (micro, macro, and the interactions between the two). The results have shown that the connectedness of the network is 23.7%, with 42.4% mutual links. The transitivity rate in the network is 51.39%, which determines the possibility of each professional communicating with a third party. According to the normalized degree index, 34.29% of the cases are in contact with other researchers in the network and 53.15% received a connection from others. Grounded theory analysis showed that five core categories including social capital, managerial factors, research, relations, and coordination affected the quality and utility of Iranian climate change professionals’ network.
Acute Care Surgeons Spend More Time than General Surgeons on the Electronic Health Record (EHR)
Authors: Loszko Abigail , Watson Michael , Khan Ahsan , Cunningham Kyle , Thomas Bradley , Ross Samuel , Lauer Cynthia , Sing Ronald , Sachdev Gaurav
Published: December 2021

Background

The paradigm of Acute Care Surgery (ACS) emerged in response to decreasing operative opportunities for trauma surgeons and increasing need for surgical coverage in disciplines to which the expertise of trauma surgeons naturally extends. While the continued evolution of this specialty remains largely beneficial, unintended consequences may have arisen along the way. One aspect of ACS that remains to be thoroughly investigated is the impact of the electronic health record (EHR). The purpose of this study is to objectively quantify EHR usage for ACS and compare it to other general surgery specialties.

Methods

EHR user data were collected for fifteen ACS attendings and thirty-seven general surgery attendings from October 2014 to September 2019. Comparative analysis was conducted using two-tailed t-tests. Subgroup analysis was conducted for subspecialties included in the general surgery group.

Results

ACS attendings opened almost 3 times as many charts as general surgery attendings per month (180 vs 64 charts/month, P < .0001), and ultimately spent more time on the EHR as a result (10 vs 6.4 hours/month, P < .0001). Documentation was the most time consuming EHR task for both groups. Although ACS attendings spent less overall time per patient chart, the proportion of time spent on certain EHR tasks was similar to that of general surgery colleagues.

Discussion

The EHR imposes a disproportionate burden on ACS attendings compared to their general surgery counterparts, and additional study is warranted to improve usage. EHR usage burden has workforce implications for trainees considering a career in ACS.
Teacher Mindsets Help Explain Where a Growth-Mindset Intervention Does and Doesn’t Work
Authors: Yeager David S. , Carroll Jamie M. , Buontempo Jenny , Cimpian Andrei , Woody Spencer , Crosnoe Robert , Muller Chandra , Murray Jared , Mhatre Pratik , Kersting Nicole , Hulleman Christopher , Kudym Molly , Murphy Mary , Duckworth Angela Lee , Walton Gregory M. , Dweck Carol S.
Published: December 2021

A growth-mindset intervention teaches the belief that intellectual abilities can be developed. Where does the intervention work best? Prior research examined school-level moderators using data from the National Study of Learning Mindsets (NSLM), which delivered a short growth-mindset intervention during the first year of high school. In the present research, we used data from the NSLM to examine moderation by teachers’ mindsets and answer a new question: Can students independently implement their growth mindsets in virtually any classroom culture, or must students’ growth mindsets be supported by their teacher’s own growth mindsets (i.e., the mindset-plus-supportive-context hypothesis)? The present analysis (9,167 student records matched with 223 math teachers) supported the latter hypothesis. This result stood up to potentially confounding teacher factors and to a conservative Bayesian analysis. Thus, sustaining growth-mindset effects may require contextual supports that allow the proffered beliefs to take root and flourish.
Taking “Thanks” for Granted: A Cross-Cultural Exploration of Gratitude in the UK and Australia
Authors: Morgan Blaire , Gulliford Liz , Waters Lea
Published: December 2021

Examinations of the influence of culture on how gratitude is experienced are sparse, as are studies that simultaneously explore developmental differences in understandings of gratitude. This paper presents three studies that examine whether perceptions and experiences of gratitude differ across children, adolescents and adults in two individualistic, WEIRD and Commonwealth cultures—Australia and the UK. Studies 1a ( N = 88, ages 17–39) and 1b ( N = 77, ages 17–25) provide initial insights into “features of gratitude” in Australia through two stages of a prototype analysis. These features are compared to a previous prototype study of gratitude in the UK, alongside a further comparison to the US. Study 2 employs vignettes to examine how perceptions of the benefactor, benefit and mixed emotions influence the degree of gratitude experienced across adolescents and adults in Australia ( N = 1937, ages 11–85), with a comparison to the UK ( N = 398, ages 12–65). In Study 3, factors examined in Study 2 are adapted into accessible story workbooks for younger children (Australia N=135, ages 9–11; UK N=62, ages 9–11). Results across these studies demonstrate similarities and differences in understandings and experiences of gratitude across cultures. While adults across Australia and the UK responded similarly to gratitude scenarios, cross-cultural differences are observed between children and adolescents in these two countries. Developmental differences are noted in relation to more sophisticated reasoning around gratitude, such as recognition of ulterior motives. These findings highlight the need for gratitude research and interventions to be cross-culturally, and developmentally, responsive.
Synergistic effects of iron and hexagonal-Boron Nitride additions in copper-based composites for braking application
Authors: Govindarajan S , Syamkumar K , Lamture Ninad , Kale Shirish S , Ram Prabhu T
Published: December 2021

This paper explores the addition of h-BN and iron to Cu-based brake pads on the performance benefits. It also investigates the effect of graded layering by synthesizing three and four-layer brake pads by powder compaction and sintering route. The top one or two layers are made of Cu-based composite containing Fe, h-BN, and W, while the middle layer is pure Cu and, bottom steel plate. Two different compositions were explored for the composites by varying Fe content. From the two composite compositions, brake pads with single-layer composite or two-layer composite were synthesized. Characterization of brake pad specimens was carried out using density measurements, optical microscopy, scanning electron microscopy, energy dispersive spectroscopy. The brake pads were subjected to simulated braking tests at braking energy/cycle of 60, 96, and 136 K Joules. Wear rate, coefficient of friction, stopping distance, stopping time, and hardness were measured and compared among other brake pads. The brake pad containing single-layer Fe rich Cu composite showed the best performance in the simulated braking tests. EDS analysis of wear debris shows the formation of iron (boride, nitride, oxide) complex which is indicative of a surface with superior dry lubricating properties. This surface is a result of synergetic interaction between h-BN and Fe particles. The iron particles which are scattered in the Cu matrix composite act as low friction regions on the brake pad surface that interrupt the high friction regions on the Cu matrix, thus reducing the local and bulk temperature rise. The two-layer composite brake-pad showed performance intermediate to the two single-layer brake pads. No advantage due to higher thermal conductivities in Fe deficient composite was observed as the two composite layers, showed similar Fe contents in their matrix phases.
Identifying data elements and key features of a mobile-based self-care application for patients with COVID-19 in Iran
Authors: Mohammad Heydari , Elham Monaghesh , Mehraeen Esmaeil , Aghamohammadi Vahideh , Seyedalinaghi Seyedahmad , Kalantari Saieed , Nahid Mehrabi , Nasiri Khadije
Published: December 2021

Mobile Health applications have shown different usages in the COVID-19 pandemic, which consisted of empowering patient’s awareness, promoting patient’s self-care, and self-monitor behaviors. The purpose of this study is to identify key features and capabilities of a mobile-based application for self-care and self-management of people with COVID-19 disease. This study was a descriptive-analytical study that was conducted in two main phases in 2020. In the first phase, a literature review study was performed. In the second phase, using the information obtained from the review of similar articles, a questionnaire was designed to validate identified requirements. Based on the results of the first phase, 53 data elements and technical key features for mobile-based self-care application for people with COVID-19 were identified. According to the statistical population, 11 data elements for demographic requirements, 11 data elements for clinical requirements, 15 data elements for self-care specifications, and 16 features for the technical capability of this app were determined. Most of the items were selected by infectious and internal medicine specialists (94%). This study supports that the use of mobile-based applications can play an important role in the management of this disease. Software design and development could help manage and improve patients’ health status.
Quality of work life, emotional and physical well-being of police personnel in India
Authors: Yadav Radha , Khanna Ashu , Chenab
Published: December 2021

The job profile of police officers places exceptional demands on them leading to risks to life, personal discomfort and stress. This article aims to examine how the physical and emotional well-being of police personnel is affected by their perceived quality of work life (QWL). The study looked at the questionnaire responses of 234 police personnel. Emotional well-being is measured as the absence of depression, emotional hyperactivity, difficulty relaxing, irritability and anxiety, whereas physical well-being is measured as the presence of diabetes, thyroid problems, insomnia and obesity. The study assessed police perceptions of QWL as provided by the government. The study findings are segregated for QWL on the basis of dominant ill-being and dominant well-being. Factors such as career and development, working environment, safety, work load, compensation and fear of punishment should be carefully analysed and improved. Some 71.8% of the respondents suffered from two or more emotional ailments; 70% suffered from two or more physical ailments. Findings suggest that QWL dimensions that fall within dominant ill-being need to be addressed immediately by policy makers and management to improve police well-being.
Transnational education provision in a time of disruption: Perspectives from Australia
Authors: Yencken Edward , Croucher Gwilym , Elliott Kristine , Locke William
Published: December 2021

As the scale and scope of transnational education (TNE) has broadened, and the range of delivery models and partnerships has continued to expand, so too have the challenges. Universities offering courses outside their own country face a growing range of legal, practical and political challenges. The significant disruption during 2020–2021 coming from the global coronavirus pandemic, and it’s effect on the provision of higher education in most countries, has further amplified many of these challenges, raising questions about what the future might hold for TNE, including in China. This article aims to establish the major enablers and challenges for TNE provision. It utilises an investigation into Australian TNE providers immediately prior to the COVID-19 pandemic as a means to analyse these factors. As a major provider of TNE, including in China and other Asian countries, Australia provides a useful example of how TNE partnerships are being facilitated, for better and worse. In particular, the article establishes that tailored and often market-specific collaborations offer the best chance of a successful partnership. The study also provides an important basis for considering how TNE will function as a key component of the ongoing response of the higher education sector to the pandemic.
The success of behavioral economics in improving patient retention within an intensive primary care practice
Authors: Groden Phillip , Capellini Alexandra , Levine Erica , Wajnberg Ania , Duenas Maria , Sow Sire , Ortega Bernard , Medder Nia , Kishore Sandeep
Published: December 2021

Background

A minority of the U.S. population comprises a majority of health care expenses. Health system interventions for high-cost populations aim to improve patient outcomes while reducing costly over-utilization. Missed and inconsistent appointments are associated with poor patient outcomes and increased health care utilization. PEAK Health— Mount Sinai’s intensive primary care clinic for high-cost patients— employed a novel behavioral economics-based intervention to reduce the rate of missed appointments at the practice. Behavioral economics has accomplished numerous successes across the health care field; the effect of a clinic-based behavioral economics intervention on reducing missed appointments has yet to be assessed.

Methods

This was a single-arm, pre-post trial conducted over 1 year involving all active patients at PEAK Health. The intervention consisted of: a) clinic signage, and b) appointment reminder cards containing behavioral economics messaging designed to increase the likelihood patients would complete their subsequent visit; appointment cards (t1) were transitioned to an identical EMR template (t2) at 6 months to boost provider utilization. The primary objective, the success of scheduled appointments, was assessed with visit adherence: the proportion of successful over all scheduled appointments, excluding those cancelled or rescheduled. The secondary objective, the consistency of appointments, was assessed with a 2-month visit constancy rate: the percentage of patients with at least one successful visit every 2 months for 1 year. Both metrics were assessed via a χ2 analysis and together define patient retention.

Results

The visit adherence rate increased from 74.7% at baseline to 76.5% (p = .22) during t1 and 78.0% (p = .03) during t2. The 2-month visit constancy rate increased from 59.5% at baseline to 74.3% (p = .01) post-intervention.

Conclusions

A low-resource, clinic-based behavioral economics intervention was capable of improving patient retention within a traditionally high-cost population. A renewed focus on patient retention— employing the metrics described here— could bolster chronic care efforts and significantly improve the outcomes of high-cost programs by reducing the deleterious effects of missed and inconsistent appointments.
Effect of antiretroviral treatment on blood-brain barrier integrity in HIV-1 infection
Authors: Anesten Birgitta , Zetterberg Henrik , Nilsson Staffan , Brew Bruce J. , Fuchs Dietmar , Price Richard W. , Gisslén Magnus , Yilmaz Aylin
Published: December 2021

Background

Blood-brain barrier (BBB) injury is prevalent in patients with HIV-associated dementia (HAD) and is a frequent feature of HIV encephalitis. Signs of BBB damage are also sometimes found in neuroasymptomatic HIV-infected individuals without antiretroviral therapy (ART). The aim of this study was to investigate the integrity of the BBB before and after initiation of ART in both neuroasymptomatic HIV infection and in patients with HAD.

Methods

We determined BBB integrity by measuring cerebrospinal fluid (CSF)/plasma albumin ratios in archived CSF samples prior to and after initiation of ART in longitudinally-followed neuroasymptomatic HIV-1-infected individuals and patients with HAD. We also analyzed HIV RNA in blood and CSF, IgG Index, CSF WBC counts, and CSF concentrations of β2-micoglobulin, neopterin, and neurofilament light chain protein (NfL).

Results

We included 159 HIV-infected participants; 82 neuroasymptomatic individuals and 77 with HAD. All neuroasymptomatic individuals (82/82), and 10/77 individuals with HAD, were longitudinally followed with a median (interquartile range, IQR) follow-up of 758 (230–1752) days for the neuroasymptomatic individuals, and a median (IQR) follow-up of 241 (50–994) days for the individuals with HAD. Twelve percent (10/82) of the neuroasymptomatic individuals and 80% (8/10) of the longitudinally-followed individuals with HAD had elevated albumin ratios at baseline. At the last follow-up, 9% (7/82) of the neuroasymptomatic individuals and 20% (2/10) of the individuals with HAD had elevated albumin ratios. ART significantly decreased albumin ratios in both neuroasymptomatic individuals and in patients with HAD.

Conclusion

These findings indicate that ART improves and possibly normalizes BBB integrity in both neuroasymptomatic HIV-infected individuals and in patients with HAD.
Construction of a novel immune-related lncRNA signature and its potential to predict the immune status of patients with hepatocellular carcinoma
Authors: Deng Min , Lin Jia-Bao , Zhao Rong-Ce , Li Shao-Hua , Lin Wen-Ping , Zou Jing-Wen , Wei Wei , Guo Rong-Ping
Published: December 2021

Background

The accuracy of existing biomarkers for predicting the prognosis of hepatocellular carcinoma (HCC) is not satisfactory. It is necessary to explore biomarkers that can accurately predict the prognosis of HCC.

Methods

In this study, original transcriptome data were downloaded from The Cancer Genome Atlas (TCGA) database. Immune-related long noncoding ribonucleic acids (irlncRNAs) were identified by coexpression analysis, and differentially expressed irlncRNA (DEirlncRNA) pairs were distinguished by univariate analysis. In addition, the least absolute shrinkage and selection operator (LASSO) penalized regression was modified. Next, the cutoff point was determined based on the area under the curve (AUC) and Akaike information criterion (AIC) values of the 5-year receiver operating characteristic (ROC) curve to establish an optimal model for identifying high-risk and low-risk groups of HCC patients. The model was then reassessed in terms of clinicopathological features, survival rate, tumor-infiltrating immune cells, immunosuppressive markers, and chemotherapy efficacy.

Results

A total of 1009 pairs of DEirlncRNAs were recognized in this study, 30 of these pairs were included in the Cox regression model for subsequent analysis. After regrouping according to the cutoff point, we could more effectively identify factors such as aggressive clinicopathological features, poor survival outcomes, specific immune cell infiltration status of tumors, high expression level of immunosuppressive biomarkers, and low sensitivity to chemotherapy drugs in HCC patients.

Conclusions

The nonspecific expression level signature involved with irlncRNAs shows promising clinical value in predicting the prognosis of HCC patients.
Promoting gender equity in very young adolescents: targeting a window of opportunity for social emotional learning and identity development
Authors: Cherewick Megan , Lebu Sarah , Su Christine , Richards Lisa , Njau Prosper F. , Dahl Ronald E.
Published: December 2021

Background

The transition from childhood to adolescence is a uniquely sensitive period for social and emotional learning in the trajectory of human development. This transition is characterized by rapid physical growth, sexual maturation, cognitive and behavioral changes and dynamic changes in social relationships. This pivotal transition provides a window of opportunity for social emotional learning that can shape early adolescent identity formation and gender norms, beliefs and behaviors. The objective of this study is to evaluate the potential of a social emotional learning intervention for very young adolescents (VYAs) to improve social emotional mindsets and skills.

Methods

Discover Learning is a social emotional learning intervention designed for VYAs (10-11 years of age) to support development of social emotional mindsets and skills from four primary schools in Dar es Salaam, Tanzania. The intervention delivered three different packages of learning experiences to three arms of the study. 528 VYAs were randomized to each of the three study arms (A-Content learning, B-Content learning and reflection, and C-Content learning, reflection and experiential practice). A quantitative survey was administered to all participants before and after the intervention to capture changes in social emotional mindsets and skills. A discrete choice experiment measured changes in gender norms, beliefs and behaviors.

Results

528 VYAs were included in the analysis. Participants in all three arms of the study demonstrated significant improvements in social emotional mindsets and skills outcomes (generosity, curiosity, growth mindset, persistence, purpose and teamwork). However, Group C (who received experiential social learning opportunities in small, mixed-gender groups and a parent and community learning components demonstrated larger treatment effects on key outcomes in comparison to Groups A and B. Results indicate Group C participants had greater change in gender equity outcomes (OR = 1.69, p = <0.001) compared to Group A (OR = 1.30, p = <0.001) and Group B (OR = 1.23, p = 0.004).

Conclusion

These findings provide evidence that social emotional learning interventions targeting VYAs can improve social emotional mindsets and skills and gender equity outcomes. The findings indicate the importance ofexperientiallearning activities in mixed-gender groups during the unique developmental window of early adolescence. The study also provides support for the inclusion of parental/caregiver and community engagement in programs designed for VYAs.

Trial registration

Retrospectively registered on July 7th, 2020.NCT0445807
Aging through the time of COVID-19: a survey of self-reported healthcare access
Authors: Peckham Allie , Pituch Keenan A. , Maxfield Molly , Guest M. Aaron , Sivanandam Shalini , Doebbeling Bradley N.
Published: December 2021

Background

Chronic conditions are common and require ongoing continuous management and preventive measures. The COVID-19 pandemic may have affected the management of chronic conditions by delaying care. We sought to understand the impact of personal characteristics (i.e., age) and healthcare factors (i.e., access to a provider) on healthcare access in a sample of Americans 50 years of age or older during COVID-19.

Method

Participants completed an online survey at the start of the COVID-19 pandemic – the Aging in the Time of COVID Survey. Questions focused on health status, health care access, COVID-19 fear, and social connectedness. Participants were recruited through social media advertisements, list serves, and snowball sampling. Data collection started in early April 2020 and concluded in late May 2020. Logistic regression models examined the results of two key access points: healthcare provider/doctor (n = 481) and medication (n = 765), with 56 and 93% of participants reporting access to a provider and medications, respectively.

Results

Individuals with an established primary care provider were much more likely to obtain access to a healthcare provider, OR = 3.81 (95% CI: 1.69, 8.77), and to receive medication, OR = 4.48 (95% CI: 1.61, 11.48), during the time of COVID-19. In addition, access to medication was (a) higher for those who were older, OR = 1.05 (95% CI: 1.01, 1.09), had a higher income (greater than 100 k compared to less than 50 k, OR = 3.04 (95% CI: 1.11, 8.98), and (b) lower for those having caregiving responsibilities, OR = 0.41 (95% CI: 0.21, 0.78), or greater social isolation, OR = 0.93 (95% CI: 0.87, 0.98).

Conclusions

Although most participants had access to medication, just over half had access to a healthcare provider when needed. Notably, health-seeking behaviors for individuals who do not have an established primary care providers as well as those who provide unpaid care, are socially isolated, and younger may require more proactive approaches to care monitoring, management, and maintenance.
Imperatorin induces autophagy and G0/G1 phase arrest via PTEN-PI3K-AKT-mTOR/p21 signaling pathway in human osteosarcoma cells in vitro and in vivo
Authors: Lv Minchao , Xu Qingxin , Zhang Bei , Yang Zhiqiang , Xie Jun , Guo Jinku , He Feixiong , Wang Wei
Published: December 2021

Background

Osteosarcoma is the third most common cancer in adolescence and the first common primary malignant tumor of bone. The long-term prognosis of osteosarcoma still remains unsatisfactory in the past decades. Therefore, development of novel therapeutic agents which are effective to osteosarcoma and are safe to normal tissue simultaneously is quite essential and urgent.

Methods

Firstly, MTT assay, cell colony formation assay, cell migration and invasion assays were conducted to evaluate the inhibitory effects of imperatorin towards human osteosarcoma cells. RNA-sequence assay and bioinformatic analysis were then performed to filtrate and assume the potential imperatorin-induced cell death route and signaling pathway. Moreover, quantitative real-time PCR assay, western blot assay and rescue experiments were conducted to confirm the assumptions of bioinformatic analysis. Finally, a subcutaneous tumor-transplanted nude mouse model was established and applied to evaluate the internal effect of imperatorin on osteosarcoma by HE and immunohistochemistry staining.

Results

Imperatorin triggered time-dependent and dose-dependent inhibition of tumor growth mainly by inducing autophagy promotion and G0/G1 phase arrest in vitro and in vivo. Besides, imperatorin treatment elevated the expression level of PTEN and p21, down-regulated the phosphorylation of AKT and mTOR. In contrast, the inhibition of PTEN using Bpv (HOpic), a potential and selective inhibitor of PTEN, concurrently rescued imperatorin-induced autophagy promotion, cell cycle arrest and inactivation of PTEN-PI3K-AKT-mTOR/p21 pathway.

Conclusions

This work firstly revealed that imperatorin induced autophagy and cell cycle arrest through PTEN-PI3K-AKT-mTOR/p21 signaling pathway by targeting and up-regulating PTEN in human osteosarcoma cells. Hence, imperatorin is a desirable candidate for clinical treatments of osteosarcoma.
miR-378d suppresses malignant phenotype of ESCC cells through AKT signaling
Authors: Peng Jie , Shi Susu , Yu Juan , Liu Jianli , Wei Haixiang , Song Haixia , Wang Shaoqiang , Li Zhejie , He Shujin , Li Lei , Zhang Hongyan , Yan Zhizhen , Zhao Ran , Liu Yukun , Liu Yanrong , Li Junjun , Zhang Renya , Wang Wei
Published: December 2021

Background

Post-resistance progress in paclitaxel (PTX) treatment remains a major challenge in tumor treatment. A high dose of PTX was used for cell lines to analyze the changes in molecular expression. The miR-378d was sharply reduced in surviving cells, but the role of miR-378d in Esophageal squamous cell carcinoma (ESCC) remained unclear.

Methods

We analyzed the relationship between miR-378d expression and the clinicopathological features of ESCC. We constructed miR-378d silent expression cell lines to study phenotypes and molecular mechanisms.

Results

The miR-378d expression was associated with good prognosis in patients with ESCC. miR-378d inhibition promoted chemo-resistance, monoclonal formation, EMT, migration, invasion, stemness, and metastasis of ESCC cells. miR-378d can target downregulated AKT1.

Conclusions

Therefore, miR-378d expression is a good prognostic factor of patients with ESCC and regulates the malignant phenotype of tumor cells through AKT.
LncRNA HCP5 as a potential therapeutic target and prognostic biomarker for various cancers: a meta‑analysis and bioinformatics analysis
Authors: Hu Shao-pu , Ge Meng-xue , Gao Lei , Jiang Min , Hu Kai-wen
Published: December 2021

Background

Accumulating studies indicated that dysregulated long non-coding RNA human histocompatibility leukocyte antigen (HLA) Complex P5 (HCP5) may functions as an potential prognostic predictor in multiple cancers. This meta-analysis was performed to systematically collect studies and conduct an evidence-based evaluation of the prognostic role of HCP5 in malignancies.

Methods

Four databases (PubMed, Web of Science, Embase and Cochrane library) were comprehensively retrieved from their initiation date to November 9, 2021. Hazard ratio (HR) or odds ratio (OR) with 95% confidence interval (CI) were used to assess the associations between the expression level of HCP5 and prognosis or clinical characteristics. Moreover, results were validated by Gene Expression Profiling Interactive Analysis 2 (GEPIA2) and the National Genomics Data Center (NGDC). Subsequently, the molecular mechanism of HCP5 was predicted based on MEM and StarBase databases. The study protocol was registered at PROSPERO (ID: CRD42021274208).

Results

9 studies, containing 641 patients, were included in this meta-analysis. Our results revealed that HCP5 overexpression was associated with poor overall survival (OS), tumor type, histological differentiation, and lymph node metastasis in most cancers, but was not associated with age, gender and tumor size; down-regulation of HCP5 was associated with worse OS, advanced tumor stage, positive distal metastasis and lymph node metastasis in skin cutaneous melanoma (SKCM). HCP5 was significantly up-regulated in four cancers and down-regulated in SKCM, which was validated by the GEPIA2 cohort. HCP5 expression in various types of cancer was also verified in NGDC. Further functional prediction revealed that HCP5 may participate in some cancer-related pathways.

Conclusion

There is a significantly association between dysregulation of HCP5 and both prognosis and clinicopathological features in various cancers. HCP5 may be functions as a novel potential prognostic biomarker and therapeutic target in multiple human cancers.
Simple method to evaluate the pixel crosstalk caused by fringing field effect in liquid-crystal spatial light modulators
Authors: Moreno Ignacio , Sánchez-López María Del Mar , Davis Jeffrey A. , Cottrell Don M.
Published: December 2021

In this work we provide a simple experimental method to measure and evaluate the pixel crosstalk in phase-only liquid-crystal displays caused by the fringing field effect. The technique is a reverse engineering method that does not require information about the microscopic physical parameters of the liquid-crystal material or details of the fabrication and electronics of the display. Instead, it is based on the overall effect on the diffraction efficiency of displayed binary phase gratings as a function of the addressed gray level. We show how the efficiency of the zero (DC) and first diffraction orders provides valuable information enough to identify and quantify the pixel crosstalk. The technique is demonstrated with a modern phase-only liquid-crystal on silicon (LCOS) spatial light modulator (SLM), illustrating the limitations that this effect imposes to the spatial resolution of the device and providing quantitative measurement of the impact on the diffraction efficiency.
Long-term observations on the mortality of mentally ill patients admitted to a short-stay psychiatric hospital: 23 years retrospective Egyptian study
Authors: Ali Dalia H. , Shorab Eman , Hassan Ghada A.M. , elRasheed Amany Haroun , Soltan Marwa Abdel Rhman , Elrassas Hanan
Published: December 2021

Background

Information about mortality patterns in different psychiatric populations is scanty, yet it is vital for designing successful preventive mental health strategies. In this study, we aimed to assess mortality rates and patterns for the patients admitted to Ain Shams University Institute of Psychiatry (ASUIP) with different psychiatric diagnoses from1990 to 2013. All medical records and related registration files were reviewed and investigated for death cases and their possible causes in a retrospective record linkage study. Data were recorded, tabulated, and coded to be used in the Statistical Package for Social Sciences (SPSS) Version 17.

Results

The study identified 57 death cases in 23-years duration with a mortality rate of 3/1000. Mortality rates were more in younger patients and females. Medical comorbidity was reported in 34.8% of them. The most common known causes for death in the current sample were cardiac causes 15.2%, followed by cerebrovascular causes that were 10.9%. However, sudden or unknown causes were the most frequent diagnosis in the sample.

Conclusions

The institute mortality rates were lower than those of the general population during this period. The most common known death causes were cardiovascular problems, which mandates close monitoring of high-risk psychiatric patients with co-morbid cardiac problems. Unknown morality causes represented an unresolved challenge for the current registration system and the quality of care given to patients with serious mental illness.
ALPLGenotypes in Patients With Atypical Femur Fractures or Other Biochemical and Clinical Signs of Hypophosphatasia
Authors: Marini Francesca , Masi Laura , Giusti Francesca , Cianferotti Luisella , Cioppi Federica , Marcucci Gemma , Ciuffi Simone , Biver Emmanuel , Toro Giuseppe , Iolascon Giovanni , Iantomasi Teresa , Brandi Maria Luisa
Published: December 2021

Context

Hypophosphatasia (HPP) is a rare metabolic disorder caused by deficiency of alkaline phosphatase (ALP) enzyme activity, leading to defective mineralization, due to pathogenic variants of the ALPL gene, encoding the tissue nonspecific alkaline phosphatase (TNSALP) enzyme. Inheritance can be autosomal recessive or autosomal dominant. An abnormal ALPL genetic test enables accurate diagnosis, avoiding the administration of contraindicated antiresorptive drugs that, in patients with HPP, substantially increase the risk of atypical femur fractures (AFFs) and worsen the fracture healing process that is usually already compromised in these patients.

Objective

Performing ALPL genetic testing to identify rare variants in suspected adult patients with HPP. Comparing frequencies of ALPL common variants in individuals with biochemical and/or clinical signs suggestive of adult HPP and non-HPP controls, and among different clinical subgroups of patients with a clinical suspicion of adult HPP.

Methods

Patients with suspected adult HPP were retrospectively selected for the genetic testing of the ALPL gene. Patients included were from 3 main European Bone Units (Florence, Naples, and Geneva); 106 patients with biochemical and/or clinical signs suggestive of a mild form of HPP were included.

Results

Genetic testing led to the identification of a heterozygote rare variant in 2.8% of cases who were initially referred as suspected osteoporosis. The analysis of frequencies of ALPL common variants showed a high prevalence (30.8%) of homozygosity in subjects who developed an AFF, in association with normal serum total ALP activity.

Conclusion

The results suggest homozygosity of common ALPL variants as a possible genetic mark of risk for these fractures.
Cranio-Orbital Approach for Single-Stage En Bloc Resection of Optic Nerve Glioma: Technical Note
Authors: Fredrickson Vance L. , Agnoletto Guilherme J. , Hollon Todd C. , Kundu Bornali , Mortimer Vance R. , Couldwell William T.
Published: December 2021

BACKGROUND AND IMPORTANCE:

There is no consensus on the optimal surgical approach for managing optic nerve gliomas. For solely intraorbital tumors, a single-stage lateral orbitotomy approach for resection may be performed, but when the nerve within the optic canal is affected, two-stage cranial and orbital approaches are often used. The authors describe their technique to safely achieve aggressive nerve resection to minimize the probability of recurrence that might affect the optic tracts, optic chiasm, and contralateral optic nerve.

CLINICAL PRESENTATION:

A 28-yr-old woman presented with painless progressive vision loss, resulting in blindness. The second of 2 transorbital biopsies was diagnostic and consistent with low-grade glioma. The lesion continued to grow on serial imaging. The patient was offered a globe-sparing operative approach, with aggressive resection of the lesion to minimize the probability of tumor recurrence, which could possibly affect vision in her contralateral eye. The patient did well postoperatively, with clean tumor margins on pathological analysis and no evidence of residual on imaging. On postoperative examination, she had a mild ptosis, which was nearly resolved at her 6-wk outpatient follow-up.

CONCLUSION:

This aggressive single-stage en bloc resection of an optic nerve glioma can achieve excellent tumor margins and preservation of extraocular muscle function.
Controlling Signal Artifact With Software Threshold Imaging for Magnetic Resonance-Guided Laser Interstitial Thermal Therapy
Authors: Liang Allison S. , Munier Sean M. , Danish Shabbar F.
Published: December 2021

BACKGROUND:

Magnetic resonance–guided laser interstitial thermal therapy (MRgLITT) uses intraoperative temperature mapping and thermal damage estimates to guide ablations of intracranial targets. In select cases, signal artifact presents at the target site and impairs intraprocedural decision-making by obscuring the visualization of both temperature imaging and the thermal damage estimate calculation. To date, the etiology and impact of signal artifact are unknown. However, user-selected MRgLITT software settings may play a role in generating artifact.

OBJECTIVE:

To assess the effect of the thresholding feature in MRgLITT software on signal artifact generation during intracranial ablations.

METHODS:

Ablations were performed with the Visualase MRI–guided Laser Ablation System (Medtronic). For each LITT procedure, raw thermal data were extracted at a reference threshold of 40 and reprocessed at 5 additional threshold values ranging from 35 to 60. Artifact growth rates relative to threshold values were derived using simple linear regressions and then assessed within the context of laser power and duration using Pearson correlations.

RESULTS:

A total of 33 patients were included, with 28 artifact-containing and 5 artifact-free cases. For artifact-containing cases, a 13% increase in artifact area occurred for every 1-point increase in threshold (R2 > 0.99). Artifact growth rates were not correlated with laser power (r = 0.15, P = .44) or duration (r = 0.0049, P = .98). One of the 5 artifact-free cases developed artifact at a threshold of 60.

CONCLUSION:

Artifact generation is likely multifactorial involving tissue properties and software settings. Operators can minimize software-introduced artifact by reducing threshold values.