bluuebunny/crossref_metadata_2025 huggingface dataset:
Nonlinear Weather–Growth Relationships Suggest Disproportional Growth Changes of Norway Spruce in the Eastern Baltic Region
Authors: Matisons Roberts , Elferts Didzis , Krišāns Oskars , Schneck Volker , Gärtner Holger , Wojda Tomasz , Kowalczyk Jan , Jansons Āris
Published: May 2021

Norway spruce (Picea abies (L.) H. Karst.) is predicted to decrease its abundance in the Eastern Baltic region as a result of climatic changes, and this process is already explicit at the southern limit of species lowland distribution. Still, there are uncertainties about the growth potential of Norway spruce within the region due to the plasticity of local populations. In this regard, an assessment of regional weather–growth responses, assuming a nonlinearity of the ecological relationship, can aid in the clarification of uncertainties regarding growth. Nonlinear regional weather–growth relationships for Norway spruce were assessed based on tree-ring widths from 22 stands spreading from Southern Finland to Northern Germany using dendrochronological methods and a generalized additive mixed model. Temporal and spatial stationarity of local linear weather–growth relationships was evaluated. Considering the drought sensitivity of Norway spruce, meteorological variables related to the summer moisture regime were the main predictors of radial increment, though conditions in winter and spring had complementary effects. Generally, the linear weather–growth relationships were spatially and temporary nonstationary, with some exceptions in Poland and Northern Germany. Explicit local specifics in the linear weather–growth relationships, which are common in the marginal parts of species’ distribution, were observed in Estonia, Latvia, and Poland. The estimated regional weather–growth relationships were mostly nonlinear, implying disproportional responses to climatic changes, particularly to intensifying drought conditions across the studied climatic gradient. Still, the responses to winter temperature suggested that warming might contribute to growth. The estimated linear and nonlinear growth responses indicate strict limitation by drought conditions, implying reductions of increment due to climatic changes southward from Latvia, suggesting the necessity for proactive management. Nevertheless, in the northern part of the analyzed region, the projected climatic changes appear favorable for growth of Norway spruce in the near future.
Diagnostic Assessment and Restoration Plan for Damaged Forest around the Seokpo Zinc Smelter, Central Eastern Korea
Authors: Kim A Reum , Lim Bong Soon , Seol Jaewon , Lim Chi Hong , You Young Han , Lee Wan Sup , Lee Chang Seok
Published: May 2021

Research Highlights: This study was carried out to diagnose the forest ecosystem damaged by air pollution and to then develop a restoration plan to be used in the future. The restoration plan was prepared by combining the diagnostic assessment for the damaged forest ecosystem and the reference information obtained from the conservation reserve with an intact forest ecosystem. The restoration plan includes the method for the amelioration of the acidified soil and the plant species to be introduced for restoration of the damaged vegetation depending on the degree of damage. Background and Objectives: The forest ecosystem around the Seokpo smelter was so severely damaged that denuded lands without any vegetation appear, and landslides continue. Therefore, restoration actions are urgently required to prevent more land degradation. This study aims to prepare the restoration plan. Materials and Methods: The diagnostic evaluation was carried out through satellite image analysis and field surveys for vegetation damage and soil acidification. The reference information was obtained from the intact natural forest ecosystem. Results: Vegetation damage was severe near the pollution source and showed a reducing trend as it moved away. The more severe the vegetation damage, the more acidic the soil was, and thereby the exchangeable cation content and vegetation damage were significantly correlated. The restoration plan was prepared by proposing a soil amelioration method and the plants to be introduced. The soil amelioration method focuses on ameliorating acidified soil and supplementing insufficient nutrients. The plants to be introduced for restoring the damaged forest ecosystem were prepared by compiling the reference information, the plants tolerant to the polluted environment, and the early successional species. The restoration plan proposed the Pinus densiflora, Quercus mongolica, and Cornuscontroversa–Juglansmandshurica communities as the reference conditions for the ridge, slope, and valley, respectively, by reflecting the topographic condition. Conclusions: The result of a diagnostic assessment showed that ecological restoration is required urgently as vegetation damage and soil acidification are very severe. The restoration plan was prepared by compiling the results of these diagnostic assessments and reference information collected from intact natural forests. The restoration plan was prepared in the two directions of soil amelioration and vegetation restoration.
Acetaldehyde Stimulation of the Growth of Zymomonas mobilis Subjected to Ethanol and Other Environmental Stresses: Effect of Other Metabolic Electron Acceptors and Evidence for a Mechanism
Authors: Vriesekoop Frank , Pamment Neville B.
Published: May 2021

Ethanol-stressed cultures of Z. mobilis showed greatly reduced lag times in growth when supplemented with small amounts of acetaldehyde. This effect could be mimicked by other metabolic electron acceptors, including propionaldehyde and oxygen, indicating a redox-based mechanism. Added propionaldehyde was rapidly and stoichiometrically converted to 1-propanol, suggesting that added acetaldehyde is also reduced during early growth. Acetaldehyde addition measurably accelerated glycolysis in nongrowing cells and also slightly stimulated cultures subjected to temperature change, osmotic shock and salt and acetate stress. Acetaldehyde’s stimulatory effect appears to be due to its ability to accelerate glycolysis via its effect on the cellular redox balance. Acetaldehyde reduction opposes the drain on NAD+ concentrations caused by oxidation of the added ethanol, accounting for the particularly strong effect on ethanol-stressed cells. This study provides evidence for our earlier proposed redox-based mechanism for acetaldehyde’s ability to reduce the lag phase of environmentally stressed cultures and suggests that the effect may have applications in industrial fermentations, especially those inhibited by ethanol and toxic compounds present in, for instance, lignocellulosic hydrolysates.
Analysis of the State of Learning in University Students with the Use of a Hadoop Framework
Authors: Villegas-Ch. William , Roman-Cañizares Milton , Sánchez-Viteri Santiago , García-Ortiz Joselin , Gaibor-Naranjo Walter
Published: May 2021

Currently, education is going through a critical moment due to the 2019 coronavirus disease that has been declared a pandemic. This has forced many organizations to undergo a significant transformation, rethinking key elements of their processes and the use of technology to maintain operations. The continuity of education has become dependent on technological tools, as well as on the ability of universities to cope with a precipitous transition to a remote educational model. That has generated problems that affect student learning. This work proposes the implementation of a Big Data framework to identify the factors that affect student performance and decision-making to improve learning. Similar works cover two main research topics under Big Data in education, the modeling and storage of educational data. However, they do not consider issues such as student performance and the improvement of the educational system with the integration of Big Data. In addition, this work provides a guide for future studies and highlights new insights and directions for the successful use of Big Data in education. Real-world data were collected for the evaluation of the proposed framework, the collection of these being the existing limitation in all research due to generalized rejection of data consent.
Foam Structure Preservation during Microwave-Assisted Vacuum Drying: Significance of Interfacial and Dielectric Properties of the Bulk Phase of Foams from Polysorbate 80–Maltodextrin Dispersions
Authors: Kubbutat Peter , Kulozik Ulrich , Dombrowski Jannika
Published: May 2021

This study aimed at examining the cause of differences in the structure preservation of polysorbate 80–maltodextrin foams during microwave-assisted vacuum drying (MWVD) versus conventional vacuum drying (CVD). Aqueous dispersions of 3% polysorbate 80 and 0–40% maltodextrin were characterized for their dielectric and interfacial properties, and results were related to their drying performance in a foamed state. Surface tension and surface dilatational properties as well as dielectric properties clearly responded to the variation in the maltodextrin content. Likewise, the foam structure preservation during CVD was linked to the maltodextrin concentration. Regarding MWVD, however, foams collapsed at all conditions tested. Nevertheless, if the structure during MWVD remained stable, the drying time was significantly reduced. Eventually, this finding could be linked to the dielectric properties of polysorbate 80 rather than its adsorption kinetics and surface film viscoelasticity as its resonant frequency fell within the working frequency of the microwave drying plant.
Assessing Nigerian Butchers’ Knowledge and Perception of Good Hygiene and Storage Practices: A Cattle Slaughterhouse Case Analysis
Authors: Okpala Charles Odilichukwu R. , Nwobi Obichukwu Chisom , Korzeniowska Małgorzata
Published: May 2021

In Nigeria, the National Agency for Food and Drug Administration and Control (NAFDAC) guides the inspection and production of beef meat and prescribes the good practices pertinent to beef products’ handling, processing, and packaging. Specifically, good hygiene practice (GHP) assures beef product safety and consumer protection, whereas good storage practice (GSP) assures the continuity of hygiene activities within the storage stages. Relevant literature about butchers’ knowledge and perception of good hygiene and storage practices within Nigeria slaughterhouses remains scant. This current study, therefore, assessed butchers’ knowledge and perception of good hygiene and storage practices through a cattle slaughterhouse case analysis. The selected cattle slaughterhouse serves the increasingly thriving Nsukka beef market in Enugu State, Nigeria. Content validation was utilised to authenticate the questionnaire items, which were administered face-to-face to the respondents (i.e., the butchers). The questionnaire included a total of 30 questions. The results showed that the butchers were male (Freq. = 100%, n = 50), acquired their knowledge informally (Freq. = 88%, n = 44), were largely with more than 5 years of slaughterhouse experience (Freq. = 82%, n = 41), and were strongly (p < 0.0001) familiar with good hygiene (Freq. = 96%, n = 48) and storage (Freq. = 98%, n = 49) practices. The butchers provided examples that demonstrated knowledge and perception aspects of GHP and GSP. The perception aspects of GHP and GSP were correlated more, compared to knowledge and knowledge versus perception. Very conscious of their knowledge and perception of good hygiene and storage practices, the butchers herein have to strive for continuous improvement in their slaughterhouse activities to assure beef quality and consumer safety.
Household Food Metabolism: Losses, Waste and Environmental Pressures of Food Consumption at the Regional Level in Spain
Authors: Di Donato Monica , Carpintero Óscar
Published: May 2021

Dealing with an increasing population is challenging the global food system not only in productive terms, but also through the associated environmental pressures. A growing diagnostic effort is being made by global and national agencies. Innovative approaches are needed to support effective policy efforts. This study aims to illustrate the potentialities of the household metabolism approach in the diagnosis of the environmental pressures derived from household food consumption, using the Spanish regions and the effects of the 2008 crisis as case studies. The direct information concerning food consumption in physical terms provided by the Spanish household budget survey is used to estimate some relevant environmental pressures (food losses and waste along the food chain, as well as water and carbon footprint) for the Spanish food system at a sub-national level. These data are directly translated into differences in environmental pressures and compared with other dietary profiles. Furthermore, the physical information of environmental pressures is related to household socio-economic status, showing the potentialities of the association with household socio-economic information. Finally, our data illustrate with some examples how the economic crisis has acted as a driver of change in food consumption, promoting a better environmental performance at the cost of poorer diets.
Gluten Assessment in Beers: Comparison by Different Commercial ELISA Kits and Evaluation of NIR Analysis as a Complementary Technique
Authors: Fernández-Gil María del Pilar , Simon Edurne , Gibert Anna , Miranda Jonatan , Roger Alcoba Esther , Martínez Olaia , Vilchez Cerezo Elisenda , Bustamante María Ángeles
Published: May 2021

Traditionally, beers are made with gluten-containing cereals. It is crucial to have rapid analytical methodologies that allow gluten content control of the beers for celiac consumers. We assess the content of gluten in 65 conventional and 41 gluten-free labeled beers commercialized in Europe and compare the results in a subgroup of 71 beers with three ELISA kits. This research allows gathering information on the potential complementary utility of NIR analysis applied to gluten analysis of gluten-free beers in terms of time saving. Results obtained with the ELISA technique identified competitive R5 to be the most sensitive in detecting the prolamins, by eliciting a higher number of beers containing gluten above 20 mg/kg. The gluten content in conventional beers tested increased with the presence of wheat as raw material and with the use of ale-type yeasts. By using competitive R5, 3 out of the 41 gluten-free labeled beers appeared to contain gluten above 20 mg/kg, and conversely, 15 out of 65 of the conventional beers showed a gluten content below this threshold. According to our approaches, NIR did not achieve a suitable correlation with ELISA results, neither for gluten quantification nor for discrimination, and therefore, it cannot be proposed as a complementary technique.
Portable Raman Spectrometer as a Screening Tool for Characterization of Iberian Dry-Cured Ham
Authors: Martín-Gómez Andrés , Arroyo-Manzanares Natalia , García-Nicolás María , López-Lorente Ángela I. , Cárdenas Soledad , López-García Ignacio , Viñas Pilar , Hernández-Córdoba Manuel , Arce Lourdes
Published: May 2021

Dry-cured Iberian ham is officially classified into different commercial categories according to the pig’s breed and feeding regime. These reach very different prices, thus promoting labelling fraud and causing great damage to the food sector. In this work, a method based on Raman spectroscopy was explored as a rapid in situ screening tool for Iberian ham samples. A total of 110 samples were analyzed to assess the potential of this technique to differentiate purebred, crossbred, acorn-fed and feed-fed dry-cured Iberian ham. A continuous signal probably due to sample fluorescence was obtained, which hid the Raman scattering signal. Therefore, chemometric treatment was applied in order to extract non-apparent information. High validated classification rates were obtained for feeding regime (83.3%) and breed (86.7%). In addition, an interlaboratory study was carried out to confirm the applicability of the method with 52 samples, obtaining a validated rate above 80%.
A Fluorescent Detection for Paraquat Based on β-CDs-Enhanced Fluorescent Gold Nanoclusters
Authors: Ren Hong-Xin , Mao Min-Xin , Li Min , Zhang Cun-Zheng , Peng Chi-Fang , Xu Jiang-Guo , Wei Xin-Lin
Published: May 2021

In this report, a fluorescent sensing method for paraquat based on gold nanoclusters (AuNCs) is proposed. It was found that paraquat could quench both glutathione-capped AuNCs (GSH-AuNCs) and β-cyclodextrin-modified GSH-AuNCs (GSH/β-CDs-AuNCs). The modification of β-CDs on the surface of GSH-AuNCs obviously enhanced the fluorescence intensity of GSH-AuNCs and improved the sensitivity of paraquat sensing more than 4-fold. This sensibilization was ascribed to the obvious fluorescence intensity enhancement of GSH-AuNCs by β-CDs and the “host–guest” interaction between paraquat and β-CDs. The fluorescence quenching was mainly due to the photoinduced energy transfer (PET) between GSH/β-CDs-AuNCs and paraquat. With the optimized β-CDs modification of the GSH-AuNC surfaces and under buffer conditions, the fluorescent detection for paraquat demonstrated a linear response in the range of 5.0–350 ng/mL with a detection limit of 1.2 ng/mL. The fluorescent method also showed high selectivity toward common pesticides. The interference from metal ions could be easily masked by ethylene diamine tetraacetic acid (EDTA). This method was applied to the measurement of paraquat-spiked water samples and good recoveries (93.6–103.8%) were obtained. The above results indicate that host molecule modification of fluorescent metal NC surfaces has high potential in the development of robust fluorescent sensors.
COVID-19′s First Wave: Examination of Impact on Food Purchasing Behaviour in the Eurozone
Authors: Gutiérrez-Villar Belén , Melero-Bolaños Rosa , Carbonero-Ruz Mariano
Published: May 2021

COVID-19 has had a negative impact on the living conditions of people in all countries worldwide. With a devastating economic crisis where many families are finding it difficult to pay bills and make ends meet, increases in prices of food basket staples can be very worrying. This study examines the relationship between the incidence of the pandemic during the first wave in 16 Eurozone countries with the variation experienced in food prices. We analysed the harmonised index of consumer food prices (included in HICP) and the classification of the degree of pandemic impact by country, the latter established with the index of deaths provided by the Johns Hopkins Center. The procedure used compared actual food prices during the first wave (March to June 2020) with those foreseeable in the absence of the pandemic. Time series analysis was used, dividing the research period into two phases. In both phases, the Holt–Winters model was applied for estimation and subsequent prediction. After a contrast using Kendall’s tau correlation index, it was concluded that in the countries with the highest death rates during the first wave, there was a higher increase in food prices than in the least affected countries of the Eurozone.
Influences of Smoking in Traditional and Industrial Conditions on Flavour Profile of Harbin Red Sausages by Comprehensive Two-Dimensional Gas Chromatography Mass Spectrometry
Authors: Yin Xiaoyu , Chen Qian , Liu Qian , Wang Yan , Kong Baohua
Published: May 2021

Smoking is mainly used to impart desirable flavour, colour and texture to the products. Various food smoking methods can be divided into traditional and industrial methods. The influences of three different smoking methods, including traditional smouldering smoke (TSS), industrial smouldering smoke (ISS) and industrial liquid smoke (ILS), on quality characteristics, sensory attributes and flavour profiles of Harbin red sausages were studied. The smoking methods had significant effects on the moisture content (55.74–61.72 g/100 g), L*-value (53.85–57.61), a*-value (11.97–13.15), b*-value (12.19–12.92), hardness (24.25–29.17 N) and chewiness (13.42–17.32). A total of 86 volatile compounds were identified by headspace solid phase microextraction combined with comprehensive two-dimensional gas chromatography mass spectrometry (GC × GC-qMS). Among them, phenolic compounds were the most abundant compounds in the all sausages. Compared with sausages smoked with smouldering smoke, the ILS sausages showed the highest content of volatile compounds, especially phenols, alcohols, aldehydes and ketones. Principal component analysis showed that the sausages smoked with different methods had a good separation based on the quality characteristics and GC × GC-qMS data. These results will facilitate optimising the smoking methods in the industrial production of smoked meat products.
Effect of Psyllium (Plantago ovata Forks) Husk on Characteristics, Rheological and Textural Properties of Threadfin Bream Surimi Gel
Authors: Singh Avtar , Benjakul Soottawat , Prodpran Thummanoon , Nuthong Pornpot
Published: May 2021

Effects of psyllium (Plantago ovata) husk powder (PHP) at various concentrations (0, 1, 2, 3 and 4%, w/w) on gelling properties of surimi from threadfin bream (Nemipterus sp.) were investigated. The addition of 1% PHP resulted in the highest increase (50%) in the breaking force (BF) of surimi gel (S), compared to that of the control gel (CON; without PHP). Lower BF was obtained for gel incorporated with PHP at the higher levels (2–4%) (p < 0.05). On the other hand, deformation (DF) was decreased with the addition of PHP at all levels compared to the CON gel. The whiteness and expressible moisture content of gels were decreased with augmenting levels of PHP (p < 0.05). Protein patterns revealed that PHP at all concentrations did not affect the polymerization of the myosin heavy chain. A loss in the elasticity of the gel was attained with the addition of PHP as indicated by decreased storage modulus (G’). A finer and more compact network was detected in gels containing 1 and 2% PHP than that found in the CON. FTIR spectra suggested that the addition of PHP influenced the secondary structure as well as functional groups of myofibrillar proteins. Based on the sensory evaluation, the surimi containing PHP at 1–3% showed a similar overall likeness score to the CON. Therefore, PHP at the optimum level could improve the gelling properties of the threadfin bream surimi with high acceptability.
Multi-Statistical Approach for the Study of Volatile Compounds of Industrial Spoiled Manzanilla Spanish-Style Table Olive Fermentations
Authors: Garrido-Fernández Antonio , Montaño Alfredo , Cortés-Delgado Amparo , Rodríguez-Gómez Francisco , Arroyo-López Francisco Noé
Published: May 2021

Table olives can suffer different types of spoilage during fermentation. In this work, a multi-statistical approach (standard and compositional data analysis) was used for the study of the volatile organic compounds (VOCs) associated with altered (butyric, sulfidic, and putrid) and non-altered (normal) Manzanilla Spanish-style table olive fermentations. Samples were collected from two industrial fermentation yards in Seville (Spain) in the 2019/2020 season. The VOC profiles of altered (n = 4) and non-altered (n = 6) samples were obtained by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS). Ninety-one VOCs were identified and grouped into alcohols (30), esters (21), carbonyl compounds (12), acids (10), terpenes (6), phenols (6), sulfur compounds (2), and others (4). The association of the VOCs with spoilage samples depended on the standard or compositional statistical methodology used. However, butyric spoilage was strongly linked by several techniques to methyl butanoate, ethyl butanoate, and butanoic acid; sulfidic spoilage with 2-propyl-1-pentanol, dimethyl sulfide, methanol, 2-methylbutanal, 2-methyl-2-butenal, ethanol, 2-methyl-3-buten-2-ol, and isopentanol, while putrid was mainly related to D-limonene and 2-pentanol. Our data contribute to a better characterisation of non-zapatera spoiled table olive fermentations and show the convenience of using diverse statistical techniques for a most robust selection of spoilage VOC markers.
Transcriptome Profile Alterations with Carbon Nanotubes, Quantum Dots, and Silver Nanoparticles: A Review
Authors: Horstmann Cullen , Davenport Victoria , Zhang Min , Peters Alyse , Kim Kyoungtae
Published: May 2021

Next-generation sequencing (NGS) technology has revolutionized sequence-based research. In recent years, high-throughput sequencing has become the method of choice in studying the toxicity of chemical agents through observing and measuring changes in transcript levels. Engineered nanomaterial (ENM)-toxicity has become a major field of research and has adopted microarray and newer RNA-Seq methods. Recently, nanotechnology has become a promising tool in the diagnosis and treatment of several diseases in humans. However, due to their high stability, they are likely capable of remaining in the body and environment for long periods of time. Their mechanisms of toxicity and long-lasting effects on our health is still poorly understood. This review explores the effects of three ENMs including carbon nanotubes (CNTs), quantum dots (QDs), and Ag nanoparticles (AgNPs) by cross examining publications on transcriptomic changes induced by these nanomaterials.
Detection of Cryptic Fragile X Full Mutation Alleles by Southern Blot in a Female and Her Foetal DNA via Chorionic Villus Sampling, Complicated by Mosaicism for 45,X0/46,XX/47,XXX
Authors: Pandelache Alison , Francis David , Oertel Ralph , Dickson Rebecca , Sachdev Rani , Ling Ling , Gamage Dinusha , Godler David E.
Published: May 2021

We describe a female with a 72 CGG FMR1 premutation (PM) (CGG 55–199) and family history of fragile X syndrome (FXS), referred for prenatal testing. The proband had a high risk of having an affected pregnancy with a full mutation allele (FM) (CGG > 200), that causes FXS through hypermethylation of the FMR1 promoter. The CGG sizing analysis in this study used AmplideX triplet repeat primed polymerase chain reaction (TP-PCR) and long-range methylation sensitive PCR (mPCR). These methods detected a 73 CGG PM allele in the proband’s blood, and a 164 CGG PM allele in her male cultured chorionic villus sample (CVS). In contrast, the Southern blot analysis showed mosaicism for: (i) a PM (71 CGG) and an FM (285–768 CGG) in the proband’s blood, and (ii) a PM (165 CGG) and an FM (408–625 CGG) in the male CVS. The FMR1 methylation analysis, using an EpiTYPER system in the proband, showed levels in the range observed for mosaic Turner syndrome. This was confirmed by molecular and cytogenetic karyotyping, identifying 45,X0/46,XX/47,XXX lines. In conclusion, this case highlights the importance of Southern blot in pre- and postnatal testing for presence of an FM, which was not detected using AmplideX TP-PCR or mPCR in the proband and her CVS.
Dissecting the Involvement of Ras GTPases in Kidney Fibrosis
Authors: Muñoz-Félix José M. , Martínez-Salgado Carlos
Published: May 2021

Many different regulatory mechanisms of renal fibrosis are known to date, and those related to transforming growth factor-β1 (TGF-β1)-induced signaling have been studied in greater depth. However, in recent years, other signaling pathways have been identified, which contribute to the regulation of these pathological processes. Several studies by our team and others have revealed the involvement of small Ras GTPases in the regulation of the cellular processes that occur in renal fibrosis, such as the activation and proliferation of myofibroblasts or the accumulation of extracellular matrix (ECM) proteins. Intracellular signaling mediated by TGF-β1 and Ras GTPases are closely related, and this interaction also occurs during the development of renal fibrosis. In this review, we update the available in vitro and in vivo knowledge on the role of Ras and its main effectors, such as Erk and Akt, in the cellular mechanisms that occur during the regulation of kidney fibrosis (ECM synthesis, accumulation and activation of myofibroblasts, apoptosis and survival of tubular epithelial cells), as well as the therapeutic strategies for targeting the Ras pathway to intervene on the development of renal fibrosis.
Detecting a Sinistral Transpressional Deformation Belt in the Zagros
Authors: Ghanbarian Mohammad Ali , Yassaghi Ali , Derakhshani Reza
Published: May 2021

The oblique collision between the northeastern margin of the Arabian platform and the Iranian microcontinent has led to transpressional deformation in the Zagros orogenic belt in the central part of the Alpine–Himalayan orogenic belt. Although previous articles have emphasized the dextral sense of shear in the Zagros orogenic belt, in this paper, using several indicators of kinematic shear sense upon field checking and microscopic thin-section studies, evidence of the development of a sinistral top-to-the NW deformation belt is presented. The mean attitudes of the foliations and lineations in this belt are 318°/55°NE and 19°/113°, respectively.
Identification of Critical Genes and Signaling Pathways in Human Monocytes Following High-Intensity Exercise
Authors: Li Pengda , Luo Li
Published: May 2021

Background: Monocytes are critical components, not only for innate immunity, but also for the activation of the adaptive immune system. Many studies in animals and humans have demonstrated that monocytes may be closely associated with chronic inflammatory diseases and be proved to be pivotal in the association between high-intensity exercise and anti-inflammation response. However, the underlying molecular mechanisms driving this are barely understood. The present study aimed to screen for potential hub genes and candidate signaling pathways associated with the effects of high-intensity exercise on human monocytes through bioinformatics analysis. Materials and Methods: The GSE51835 gene expression dataset was downloaded from the Gene Expression Omnibus database. The dataset consists of 12 monocyte samples from two groups of pre-exercise and post-exercise individuals. Identifying differentially expressed genes (DEGs) with R software, and functional annotation and pathway analyses were then performed with related web databases. Subsequently, a protein–protein interaction (PPI) network which discovers key functional protein and a transcription factors-DEGs network which predicts upstream regulators were constructed. Results: A total of 146 differentially expressed genes were identified, including 95 upregulated and 51 downregulated genes. Gene Ontology analysis indicated that in the biological process functional group, these DEGs were mainly involved in cellular response to hydrogen peroxide, response to unfolded protein, negative regulation of cell proliferation, cellular response to laminar fluid shear stress, and positive regulation of protein metabolic process. The top five enrichment pathways in a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were the FoxO signaling pathway, protein processing in the endoplasmic reticulum, influenza A, the ErbB signaling pathway, and the MAPK signaling pathway. TNF, DUSP1, ATF3, CXCR4, NR4A1, BHLHE40, CDKN1B, SOCS3, TNFAIP3, and MCL1 were the top 10 potential hub genes. The most important modules obtained in the PPI network were performed KEGG pathway analysis, which showed that these genes were mainly involved in the MAPK signaling pathway, the IL-17 signaling pathway, the TNF signaling pathway, osteoclast differentiation, and apoptosis. A transcription factor (TF) target network illustrated that FOXJ2 was a critical regulatory factor. Conclusions: This study identified the essential genes and pathways associated with exercise and monocytes. Among these, four essential genes (TNF, DUSP1, CXCR4, and NR4A1) and the FoxO signaling pathway play vital roles in the immune function of monocytes. High-intensity exercise may improve the resistance of chronic inflammatory diseases by regulating the expression of these genes.
Correction of Breech Presentation with Moxibustion and Acupuncture: A Systematic Review and Meta-Analysis
Authors: Liao Jian-An , Shao Shih-Chieh , Chang Chian-Ting , Chai Pony Yee-Chee , Owang Kok-Loon , Huang Tse-Hung , Yang Chung-Han , Lee Tsai-Jen , Chen Yung-Chih
Published: May 2021

Acupuncture-type interventions (such as moxibustion and acupuncture) at Bladder 67 (BL67, Zhiyin point) have been proposed to have positive effects on breech presentation. The aim of this systematic review and meta-analysis was to evaluate the effectiveness and safety of moxibustion and acupuncture in correcting breech presentation. We searched PubMed, MEDLINE, Embase, the Cochrane Central Register of Controlled Trials (CENTRAL), the Chinese Electronic Periodical Services (CEPS), and databases at ClinicalTrials.gov to identify relevant randomized controlled trials (RCTs). In this study, sixteen RCTs involving 2555 participants were included. Compared to control, moxibustion significantly increased cephalic presentation at birth (RR = 1.39; 95% CI = 1.21–1.58). Moxibustion also seemed to elicit better clinical outcomes in the Asian population (RR = 1.42; 95% CI = 1.21–1.67) than in the non-Asian population (RR = 1.20; 95% CI = 1.01–1.43). The effects of acupuncture on correcting breech presentation after sensitivity analysis were inconsistent relative to control. The effect of moxibustion plus acupuncture was synergistic for correcting breech presentation (RR = 1.53; 95% CI = 1.26–1.86) in one RCT. Our findings suggest that moxibustion therapy has positive effects on correcting breech presentation, especially in the Asian population.
The Role of High Flow Nasal Cannula in COVID-19 Associated Pneumomediastinum and Pneumothorax
Authors: Simioli Francesca , Annunziata Anna , Polistina Giorgio Emanuele , Coppola Antonietta , Di Spirito Valentina , Fiorentino Giuseppe
Published: May 2021

Background: Pneumomediastinum, subcutaneous emphysema and pneumothorax are not rarely observed during the COVID-19 pandemic. Such complications can worsen gas exchange and the overall prognosis in critical patients. The aim of this study is to investigate what predisposing factors are related to pneumomediastinum and pneumothorax in SARS-CoV2-Acute Respiratory Distress Syndrome (ARDS), what symptoms may predict a severe and potentially fatal complication and what therapeutical approach may provide a better outcome. Methods: In this single center cohort study, we recorded data from 45 critically ill COVID-19 patients who developed one or more complicating events among pneumomediastinum, subcutaneous emphysema and pneumothorax. All patients showed ARDS and underwent non-invasive ventilation (NIV) at baseline. Patients with mild to moderate ARDS and pneumomediastinum/pneumothorax (n = 25) received High Flow Nasal Cannula (HFNC), while patients with severe ARDS and pneumomediastinum/pneumothorax underwent HFNC (n = 10) or invasive mechanical ventilation (IMV) (n = 10). Results: Pneumomediastinum/pneumothorax developed in 10.5% of subjects affected by SARS-coV2-ARDS. Dyspnea affected 40% and cough affected 37% of subjects. High resolution computed tomography of the chest showed bilateral diffuse ground glass opacities (GGO) in 100% of subjects. Traction bronchiolectasis, reticulation, crazy paving and distortion were observed in 64%. Furthermore, 36% showed subcutaneous emphysema. Non-severe ARDS cases received HFNC, and 76% patients recovered from pneumomediastinum/pneumothorax over a median follow up of 5 days. Among severe ARDS cases the recovery rate of pneumomediastinum/pneumothorax was 70% with the HFNC approach, and 10% with IMV. Conclusion: HFNC is a safe and effective ventilatory approach for critical COVID-19 and has a positive role in associated complications such as pneumomediastinum and pneumothorax.
Texture-Modified Diets, Nutritional Status and Mealtime Satisfaction: A Systematic Review
Authors: Wu Xiaojing Sharon , Miles Anna , Braakhuis Andrea J.
Published: May 2021

While the association between dysphagia and malnutrition is well established, there is a lack of clarity regarding the nutritional status and mealtime satisfaction of those consuming texture-modified diets (TMDs). This systematic review summarises and critically appraises the nutritional status and mealtime satisfaction of adults consuming TMDs. A systematic database search following PICO criteria was conducted using Cochrane Central (via Ovid), MEDLINE, CINAHL, EMBASE and Scopus. Nutritional status, mealtime satisfaction and costs were identified as primary outcomes. Eligible studies were grouped according to outcome measurement. In total, 26 studies met the inclusion criteria. Twenty studies evaluated the nutritional status by weight change or using malnutrition screening tools and found the consumption of TMDs correlated with weight loss or malnutrition. Nine studies evaluated mealtime satisfaction, with two reporting poor satisfaction for people on thickened fluids (TFs). Nutrition intervention through adjusting texture and consistency and nutrition enrichment showed positive effects on weight and mealtime satisfaction. The majority of the studies were rated as ‘neutral’ quality due to the limited number of experiments. TMD consumers had compromised nutritional status and poor mealtime satisfaction. More research input is required to identify promising strategies for improving the nutritional status and mealtime satisfaction of this population. Food services need to consider texture, consistency and fortification in designing menus for people on TMDs to avoid weight loss and malnutrition, and to enhance mealtime enjoyment.
Effectiveness of Testing and Contact-Tracing to Counter COVID-19 Pandemic: Designed Experiments of Agent-Based Simulation
Authors: Kim Young Jin , Koo Pyung-Hoi
Published: May 2021

The widespread outbreak of the novel coronavirus disease COVID-19 has posed an enormous threat to global public health. A different set of policy interventions has been implemented to mitigate the spread in most countries. While the use of personal protective equipment and social distancing has been specifically emphasized, South Korea has deployed massive testing and contact-tracing program from the early stage of the outbreak. This study aims at investigating the effectiveness of testing and contact-tracing to counter the spread of infectious diseases. Based on the SEICR (susceptible-exposed-infectious-confirmed-recovered) model, an agent-based simulation model is developed to represent the behavior of disease spreading with the consideration of testing and contact-tracing in place. Designed experiments are conducted to verify the effects of testing and contact tracing on the peak number of infections. It has been observed that testing combined with contact tracing may lower the peak infections to a great extent, and it can thus be avoided for the hospital bed capacity to be overwhelmed by infected patients. It is implied that an adequate capability of testing and contact-tracing may enable us to become better prepared for an impending risk of infectious diseases.
Medication Handling and Storage among Pilgrims during the Hajj Mass Gathering
Authors: Yezli Saber , Yassin Yara , Mushi Abdulaziz , Balkhi Bander , Stergachis Andy , Khan Anas
Published: May 2021

We aimed to investigate the knowledge and practices of Hajj pilgrims regarding medication storage and handling during the Hajj mass gathering. In this cross-sectional study, adult pilgrims from 30 countries were interviewed using a structured questionnaire during the 2019 Hajj. The study enrolled 1221 participants with a mean age of 50.8 years (SD = 12.5, range = 18–98) and male:female ratio of 1.7:1. Most pilgrims were literate, 50.4% had a university or higher education, and 38% reported at least one underlying health condition. Most pilgrims reported receiving education regarding the proper way to store their medication during Hajj, mainly from physicians (73.7%) and pharmacists (39.4%). Although 68.2% of pilgrims had good knowledge regarding medication storage and the potential effect of inappropriate storage conditions on medications and health, inadequate knowledge and poor practice were identified among some. Level of education, having an underlying health condition and receiving health education on mediation storage were independently associated with good knowledge. Most pilgrims took their medications with them during Hajj, although storage and handling of their medication also varied depending on the stages of their Hajj pilgrimage journey. Improving Hajj pilgrims’ awareness and knowledge about appropriate storage and handling of their medications are beneficial in reducing the risk of associated adverse health outcomes, both during Hajj and beyond the mass gathering.
The Role of Glass Additives in the Properties of Lime-Based Grouts
Authors: Pachta Vasiliki
Published: May 2021

Various types of additives have been diachronically used in historic mortars, whereas their use in lime-based grouts, used for the restoration of historic structures, is rather limited. In this study, an effort was made to investigate the performance of glass additives in grouts, consisting of lime and natural pozzolan. The additives concerned glass fibers (3 and 6 mm in length), glass beads and bubbles, added in a proportion 0.3–3% w/w of binders. Five grout compositions were manufactured and their properties at fresh and hardened states were tested. From the evaluation of the results, it was asserted that glass additives influenced the grouts’ performance in a favorable way. Their fresh state and physical properties were maintained or slightly improved, while there was a significant enhancement of the long-term mechanical characteristics of the mixes. Additionally, the adhesion capability of grouts was notably increased, which is a crucial aspect indicating their efficacy.
Tobacco Control as an LGBTQ+ Issue: Knowledge, Attitudes, and Recommendations from LGBTQ+ Community Leaders
Authors: Acosta-Deprez Veronica , Jou Judy , London Marisa , Ai Mike , Chu Carolyn , Cermak Nhi , Kozlovich Shannon
Published: May 2021

Tobacco companies use price discounts, including coupons and rebates, to market their products. Lesbian, gay, bisexual, transgender, and queer (LGBTQ+) communities are targeted by these marketing strategies, contributing to inequitably high tobacco use. Some localities have adopted policies restricting tobacco price discounts; for successful implementation, community buy-in is crucial. From July–October 2018, Equality California staff conducted semi-structured interviews with seven participants in Los Angeles, CA. Themes included familiarity with tobacco price discounts, their perceived impact on tobacco use in LGBTQ+ communities, and attitudes toward potential policy restrictions. Interview notes were analyzed using a deductive approach to qualitative analysis. Awareness of tobacco price discounts varied; some interviewees were familiar, while others expressed surprise at their ubiquity. Price discounts were seen to disproportionately impact LGBTQ+ individuals, especially those who additionally identify with other vulnerable groups, including young people and communities of color. Support for policy restrictions was unanimous; however, interviewees expressed concern over political opposition and emphasized a need for culturally competent outreach to LGBTQ+ communities. Community organizations are essential in mobilizing support for policy reform. Understanding the perceptions and recommendations of community leaders provides tools for policy action, likely improving outcomes to reduce LGBTQ+ tobacco use through restricting tobacco price discounts.
Short-Term Effects of COVID-19 Lockdown in Italian Children and Adolescents with Type 1 Diabetes Mellitus: The Role of Separation Anxiety
Authors: Di Riso Daniela , Bertini Simone , Spaggiari Silvia , Olivieri Francesca , Zaffani Silvana , Comerlati Lara , Marigliano Marco , Piona Claudia , Maffeis Claudio
Published: May 2021

In March 2020, the Italian Government imposed mandatory home confinement to limit the spread of COVID-19. Few studies assessed the psychophysical impact of COVID-19 on chronically ill children. This study examined these effects on children with Type 1 Diabetes Mellitus (T1D) and their caregivers. Seventy-one patients (7–13 years) with T1D and their caregivers were administered a survey created ad hoc and some standardized questionnaires, assessing psychological well-being and anxiety. Medical data (physical and biochemical characteristics) were recorded before (T0, January–February) and after (T1, May–June) the lockdown. Paired Student t-test, Spearman two-tailed correlations, and a linear regression model were used for statistical analysis. Children at T1 showed higher BMI (body mass index), daily total and basal insulin dose, and time spent in therapeutic range, and they showed lower HbA1c (glycated hemoglobin), time spent above the therapeutic range, and standard deviations of the mean glucose values than at T0. A total of 32.9% scored in the clinical range for separation anxiety. The increase in separation anxiety was predicted by younger age, female gender, more recent T1D diagnosis, less time spent in therapeutic range at T1, and higher perceived fear of COVID-19 infection. In a pandemic context, separation anxiety may be stronger in younger females, with more recent T1D diagnosis and poor metabolic control, thus affecting the parent’s ability to manage diabetes and to support children’s autonomy.
Identification of Hub Genes in Different Stages of Colorectal Cancer through an Integrated Bioinformatics Approach
Authors: Patil Abhijeet R. , Leung Ming-Ying , Roy Sourav
Published: May 2021

Colorectal cancer (CRC) is the third most common cancer that contributes to cancer-related morbidity. However, the differential expression of genes in different phases of CRC is largely unknown. Moreover, very little is known about the role of stress-survival pathways in CRC. We sought to discover the hub genes and identify their roles in several key pathways, including oxidative stress and apoptosis in the different stages of CRC. To identify the hub genes that may be involved in the different stages of CRC, gene expression datasets were obtained from the gene expression omnibus (GEO) database. The differentially expressed genes (DEGs) common among the different datasets for each group were obtained using the robust rank aggregation method. Then, gene enrichment analysis was carried out with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Finally, the protein-protein interaction networks were constructed using the Cytoscape software. We identified 40 hub genes and performed enrichment analysis for each group. We also used the Oncomine database to identify the DEGs related to stress-survival and apoptosis pathways involved in different stages of CRC. In conclusion, the hub genes were found to be enriched in several key pathways, including the cell cycle and p53 signaling pathway. Some of the hub genes were also reported in the stress-survival and apoptosis pathways. The hub DEGs revealed from our study may be used as biomarkers and may explain CRC development and progression mechanisms.
Social and Behavioral Correlates of Adolescent Sexual Experience and Intention to Use Condoms in Northwestern Botswana
Authors: Barchi Francis , Apps Helen , Ntshebe Oleosi , Ramaphane Peggie
Published: May 2021

Adolescent sexual behavior is shaped by individual, social, and structural factors that can increase HIV-risk, unwanted pregnancy, and sexually transmitted disease. To inform the development of a comprehensive sexuality education program, 239 secondary school adolescents ages 14–19 in Maun, Botswana, completed a survey of sexual and reproductive health knowledge, attitudes, and behaviors in February–March 2020. Bivariate and multivariate analyses examined factors associated with sexual experience and perceived ability to insist on condoms. Approximately 21% of respondents reported having had sexual intercourse. More than half felt able to insist on condoms. Sources of information about human reproduction, alcohol use, attitudes about when sex is acceptable, and perceived sexual activity by one’s peers were predictive of sexual experience. Age, confidence in correct condom use, perceived acceptability of adolescent sex with condoms, and endorsement of prevailing gender norms were significantly associated with perceived ability to insist on condom use.