bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictive Complexity of Quantum Subsystems
Authors: Asplund Curtis T. , Panciu Elisa
Published: December 2024

We define predictive states and predictive complexity for quantum systems composed of distinct subsystems. This complexity is a generalization of entanglement entropy. It is inspired by the statistical or forecasting complexity of predictive state analysis of stochastic and complex systems theory but is intrinsically quantum. Predictive states of a subsystem are formed by equivalence classes of state vectors in the exterior Hilbert space that effectively predict the same future behavior of that subsystem for some time. As an illustrative example, we present calculations in the dynamics of an isotropic Heisenberg model spin chain and show that, in comparison to the entanglement entropy, the predictive complexity better signifies dynamically important events, such as magnon collisions. It can also serve as a local order parameter that can distinguish long and short range entanglement.
Quantum Collapse and Computation in an Everett Multiverse
Authors: Tamburini Fabrizio , Licata Ignazio
Published: December 2024

The mathematical representation of the universe consists of sequences of symbols, rules and operators containing Gödel’s undecidable propositions: information and its manipulation, also with Turing Machines. Classical information theory and mathematics, ideally independent from the medium used, can be interpreted realistically and objectively from their correspondence with quantum information, which is physical. Each representation of the universe and its evolution are, in any case, physical subsets of the universe, structured sets of observers and their complements in the universe made with spacetime events generated by local quantum measurements. Their description becomes a semantically closed structure without a global object-environment loss of decoherence as a von Neumann’s universal constructor with a semantical abstract whose structure cannot be decided deterministically a priori from an internal observer. In a semantically closed structure, the realization of a specific event that writes the semantical abstract of the constructor is a problem of finding “which way” for the evolution of the universe as a choice of the constructor’s state in a metastructure, like the many-world Everett scenario, from a specific result of any quantum measurement, corresponding to a Gödel undecidable proposition for an internal observer.
Complexity Analysis and Control of Output Competition in a Closed-Loop Supply Chain of Cross-Border E-Commerce Under Different Logistics Modes Considering Chain-to-Chain Information Asymmetry
Authors: Xie Feng-Jie , Wen Lu-Ying , Cui Wen-Tian , Shen Xiao-Yang
Published: December 2024

To investigate the dynamic complexity of chain-to-chain output decisions in a closed-loop supply chain system of cross-border e-commerce (CBEC), this study decomposes the system into four product–market (PM) chains, based on the e-commerce platform’s information-sharing strategy and the manufacturer’s selected logistics mode (direct mail or bonded warehouse). By combining game theory with complex systems theory, discrete dynamic models for output competition among PM chains under four scenarios are constructed. The Nash equilibrium solution and stability conditions of the models are derived according to the principles of nonlinear dynamics. The stability of the model under the four scenarios, as well as the impacts of the initial output level and comprehensive tax rates on the stability and stability control of the system, are analyzed using numerical simulation methods. Our findings suggest that maintaining system stability requires controlling the initial output levels, the output adjustment speeds, and tariff rates to remain within specific thresholds. When these thresholds are exceeded, the entropy value of the model increases, and the system outputs decisions to enter a chaotic or uncontrollable state via period-doubling bifurcations. When the output adjustment speed of the four PM chains is high, the direct-mail logistics mode exhibits greater stability. Furthermore, under increased tariff rates for CBEC, the bonded warehouse mode has a stronger ability to maintain stability in system output decisions. Conversely, when the general import tax rate increases, the direct-mail mode demonstrates better stability. Regardless of the logistics mode, the information-sharing strategy can enhance the stability of system output decisions, while increased e-commerce platform commission rates tend to reduce stability. Interestingly, the use of a non-information-sharing strategy and the direct-mail logistics mode may be more conducive to increasing the profit levels of overseas manufacturers. Finally, the delayed feedback control method can effectively reduce the entropy value, suppress chaotic phenomena in the system, and restore stability to output decisions from a fluctuating state.
Frustrated Synchronization of the Kuramoto Model on Complex Networks
Authors: Ódor Géza , Deng Shengfeng , Kelling Jeffrey
Published: December 2024

We present a synchronization transition study of the locally coupled Kuramoto model on extremely large graphs. We compare regular 405 and 1004 lattice results with those of 12,0002 lattice substrates with power-law decaying long links (ll). The latter heterogeneous network exhibits ds>4 spectral dimensions. We show strong corrections to scaling and mean-field type of criticality at d=5, with logarithmic corrections at d=4 Euclidean dimensions. Contrarily, the ll model exhibits a non-mean-field smeared transition, with oscillating corrections at similarly high spectral dimensions. This suggests that the network heterogeneity is relevant, causing frustrated synchronization akin to Griffiths effects.
26 Years of Skin Banking in Finland
Authors: Antila Kaarle , Vuola Jyrki , Lindford Andrew
Published: December 2024

Autologous skin grafts are the gold standard for definitive wound coverage in burn care, but allograft skin grafts are essential for providing temporary coverage in cases of extensive burns. The Helsinki Skin Bank, established in 1995 at the Helsinki Burn Centre, is Finland’s only licensed skin bank, serving a population of 5.5 million. It procures human skin allografts from multi-organ donors in the Greater Helsinki area and preserves them using glycerol, a method pioneered by the Euro Skin Bank. Between 2009 and 2020, the Helsinki Skin Bank procured skin from 263 donors and provided allografts to 248 patients, primarily burn victims. Over time, procurement methods have improved significantly, resulting in an increase in the amount of skin harvested per donor. Despite rising costs due to more stringent European Union regulations and the need for round-the-clock operations, the bank has remained cost-effective. The glycerol preservation method ensures microbiological safety and effective storage, with minimal contamination issues. The future may see advances in skin substitutes and stem cell treatments, but for now, allogenic skin remains crucial in burn care due to its availability, ease of use, and cost-efficiency. Running a small, professional skin bank for a single burn center has proven successful and sustainable.
Teachers and Teaching in Teacher Education: Editorial
Authors: Guberman Ainat , Symeonidis Vasileios
Published: December 2024

A decade has passed since Goodwin and colleagues asked who teacher educators are and what they need to know and be able to do [...]
Polyimide-Based Dielectric Materials for High-Temperature Capacitive Energy Storage
Authors: Liu Lingling , Li Li , Zhang Shixian , Xu Wenhan , Wang Qing
Published: December 2024

Polyimide (PI) has received great attention for high-temperature capacitive energy storage materials due to its remarkable thermal stability, relatively high breakdown strength, strong mechanical properties, and ease of synthesis and modification. In this review, several key parameters for evaluating capacitive energy storage performance are introduced. Subsequently, the properties of the commercially available PIs are presented. Then, the recent development of designing and tailoring all-organic PI-based polymers is discussed in detail, focusing on molecular composition and spatial configuration to enhance dielectric constant, breakdown strength, discharged energy density, and charge-discharge efficiency. Finally, we outline the current challenges and future development directions of PI-based high-temperature energy storage dielectric materials.
A Closed-Loop Economy in the Meat Industry for Generating Alternative Energy from Biogas Plants
Authors: Szymańska Elżbieta Jadwiga , Mroczek Robert , Drożdż Jadwiga
Published: December 2024

This study deals with the concept of a closed-loop economy in the meat industry through the use of animal by-products in biogas plants for electricity generation processes. The purpose of the study was to identify the volume of by-products from the slaughter of cattle and pigs, how the by-products are managed, and the efficiency of their use in biogas plants for electricity production processes. The analyses used data from the Central Statistical Office, the Chief Veterinary Inspectorate, and information obtained from the Polish Agency for Enterprise Development. Studies from the literature on closed-loop economies and bioeconomies were also a valuable source of information. Based on case studies, this paper also presents examples of a closed-loop economy in meat companies. In the area of pork, poultry, and beef livestock production, the analysis covered the years 2004–2022, while in the area of the production and use of meat industry by-products, the data came from 2017–2022. The study shows that reducing the production of waste in the meat industry and the reuse and recycling of waste are important parts of a closed-loop economy. In meat companies, waste from slaughtering animals can be properly managed, either by processing it into animal feed or by using it in biogas plants to generate electricity or heat. Taking into account the number of by-products from cattle and pig slaughter in the country, biogas plants could produce approximately 95,232 MW of electricity per year, which constitutes approximately 4.3% of the energy intensity of the meat industry in Poland.
Recent Developments and Trends in High-Performance PMSM for Aeronautical Applications
Authors: Liao Chendong , Bianchi Nicola , Zhang Zhuoran
Published: December 2024

Permanent magnet synchronous machines (PMSMs) have been widely used in various applications such as robotics, electric vehicles, and aerospace due to their fast dynamic response, high-power/torque density, and high efficiency. These features make them attractive candidates for aeronautical applications, where the weight and volume of onboard systems are critically important. This paper aims to provide an overview of recent developments in PMSMs. Key design considerations for aeronautical PMSMs across different applications are highlighted based on the analysis of industrial cases and research literature. Additionally, emerging techniques that are vital in enhancing the performance of aeronautical PMSMs are discussed.
Digging Deeper: Assessing the Impact of Limestone Exploitation and Use Worldwide
Authors: Carrasqueira Joana , Afonso Clélia , Gil Maria Manuel , Bernardino Raul , Gamboa Roberto , Barroso Sónia Duarte , Bernardino Susana
Published: December 2024

Limestone has always been widely used in various sectors, thus having significant economic and environmental impacts. The quarrying process is, on the one hand, essential, but on the other, harmful to the environment and people’s health, therefore, the challenge is finding a balance. This review explores the multifaceted nature of limestone, a sedimentary rock predominantly composed of calcium carbonate. The formation of limestone, along with its various types and global occurrence, is discussed to provide a basic understanding. The diverse applications of limestone range from historical uses in ancient monuments to contemporary roles in cement production, plasticized polyvinyl chloride (PVC), and soda–lime glass. The discussion extends to post-mining solutions, showcasing successful rehabilitation and innovative repurposing of former quarries into useful infrastructures, wildlife habitats, and cultural spaces. This article draws attention to the importance of sustainable practices and technological developments to balance resource use with environmental conservation. Ultimately, it emphasizes the need for responsible limestone quarrying and proactive rehabilitation strategies to ensure that no permanent ecological problems will occur.
Cellulose Nanofibers as Rheological Modifiers to Improve Biomass Slurry Processing and Fermentation
Authors: Jamieson Zachary , Clar Jordi Francis , Runge Troy
Published: December 2024

This study investigates the enhancement of biomass slurry processability through the addition of rheological modifiers, focusing on carboxymethyl cellulose (CMC) and TEMPO-mediated oxidized cellulose nanofibrils (TCNF). Three sets of experiments were conducted to assess the effects of these additives on slurry processing and fermentation. Initial experiments evaluated the slurry extrudability, concluding that TCNF aids extrusion similarly to CMC. Subsequent experiments explored slurry viscosity reduction mechanisms, revealing that while CMC significantly reduced the viscosity, TCNF’s impact is negligible. Additionally, TCNF performed comparably to CMC in water retention tests across different conditions, which suggests that TCNF have potential as an effective additive for maintaining slurry fluidity at high solid concentrations through enhanced water retention. Lastly, both additives were investigated to ensure that they did not impact hydrolyzed biomass fermentation. The findings suggest that TCNF’s mechanisms differ from those of traditional water-soluble polymers like CMC, offering insights into novel approaches to improve the biomass processing efficiency and subsequent fermentation.
Assessment of Characteristic Flavor and Taste Quality of Sugarcane Wine Fermented with Different Cultivars of Sugarcane
Authors: Yang Yuxia , Chen Jing , Zheng Fengjin , Lin Bo , Wu Feifei , Verma Krishan K. , Chen Ganlin
Published: December 2024

In order to explore the variation in volatile compounds and aroma profiles of different varieties of sugarcane wine, volatile compounds of 14 different varieties of sugarcane wine were analyzed by headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS) and an electronic sensory system. The differences in flavor substances of different cultivars of sugarcane were assessed by orthogonal partial least squares discriminant analysis (OPLS-DA) discriminant model and relative odor activity value (ROAV) combined with multivariate statistical methods. The results showed that a total of sixty major volatile compounds, i.e., 27 esters, 15 alcohols, eight acids, three phenols, four aldehydes and ketones, and four others, were identified in 14 types of sugarcane wine. Seven key aroma compounds were screened out: ethyl caprylate, ethyl caprate, ethyl acetate, ethyl laurate, n-decanoic acid, 2,4-di-tert-butylphenol, and 2-phenylethanol and three differential aromas, i.e., ethyl palmitate, isobutyl alcohol, and caprylic acid. The electronic nose and electronic tongue analysis technology can effectively distinguish the aroma and taste of 14 sugarcane wines. It is confirmed that the aroma and taste of 14 sugarcane wines have differences in distribution patterns, and the results are consistent with the analysis and assessment of volatile compounds of sugarcane wine. The results of this study provide technical support for the production and quality improvement of sugarcane wine.
Effects of Wildfire Exposure on the Human Immune System
Authors: Frumento Davide , Țãlu Ștefan
Published: December 2024

Wildfires have become a significant environmental and public health concern worldwide, particularly due to their increased frequency and intensity driven by climate change. Wildfire smoke, composed of a complex mixture of particulate matter, gases and chemicals, has been linked to numerous health issues, primarily affecting the respiratory and cardiovascular systems. However, emerging evidence suggests that wildfire smoke exposure also has profound effects on the immune system. This review aims to synthesize current knowledge on how wildfire smoke exposure affects the human immune system, including acute and chronic impacts, underlying mechanisms and potential long-term consequences. The review discusses the role of inflammation, oxidative stress and immune cell modulation in response to wildfire smoke, highlighting the need for further research to fully understand these effects.
Value-Added Foods: Composition, Sensory, and Consumer Research
Authors: Prinyawiwatkul Witoon
Published: December 2024

The term “value-added” continues to be a relevant topic for global research, especially when it is related to increased economic value, increased consumer satisfaction, food waste reduction, and sustainability [...]
Preparation and Characterization of Small-Size and Strong Antioxidant Nanocarriers to Enhance the Stability and Bioactivity of Curcumin
Authors: Tie Shanshan , Yang Yujin , Ding Jiawei , Li Yanyan , Xue Mengmeng , Sun Jianrui , Li Fang , Fan Qiuxia , Wu Ying , Gu Shaobin
Published: December 2024

The purpose of this study was to design nanocarriers with small-size and antioxidant properties for the effective encapsulation of curcumin. Here, procyanidins, vanillin, and amino acids were used to successfully prepare nanocarriers of a controllable size in the range of 328~953 nm and to endow antioxidant ability based on a one-step self-assembly method. The reaction involved a Mannich reaction on the phenolic hydroxyl groups of procyanidins, aldehyde groups of vanillin, and amino groups of amino acids. Subsequently, curcumin nanoparticles were prepared by loading curcumin with this nanocarrier, and the encapsulation efficiency of curcumin was 85.97%. Compared with free curcumin, the antioxidant capacity and photothermal stability of the embedded curcumin were significantly improved, and it could be slowly released into simulated digestive fluid. Moreover, using the corticosterone-induced PC12 cell injury model, the cell viability increased by 23.77% after the intervention of curcumin nanoparticles, and the cellular antioxidant capacity was also significantly improved. The nanoparticles prepared in this work can effectively improve the solubility, stability, and bioactivity of curcumin, which provides a reference for the embedding and delivery of other hydrophobic bioactive compounds.
Impact of Conventional Pasteurization, High Temperature Short Time, Ultra-High Temperature, and Storage Time on Physicochemical Characteristics, Bioactive Compounds, Antioxidant Activity, and Microbiological Quality of Fruit Nectars
Authors: Polak Natalia , Kalisz Stanisław , Hać-Szymańczuk Elżbieta , Kruszewski Bartosz
Published: December 2024

Berries are a valuable source of numerous bioactive compounds, and they have an interesting organoleptic profile. Unfortunately, their low storage life determines the need for their preservation. Among the various methods used in this regard, it was decided to use the High Temperature Short Time (HTST) (90 °C/15 s) and Ultra-High Temperature (UHT) (130 °C/5 s) methods to preserve the produced fruit nectar blends (strawberry–blackcurrant and strawberry–chokeberry). For comparison, the nectars were also preserved using conventional pasteurization (90 °C/10 min). Physicochemical, chromatographic, and microbiological determinations were carried out in the tested nectars before and immediately after processing, as well as after 1, 2, 3, 4, and 6 months of refrigerated storage. All methods allowed for the significant inactivation of selected microbial groups. Non-significant changes were observed as a result of HTST and UHT processing in the context of pH, TSS, and titratable acidity. Varied major changes occurred in the content of bioactive components (TPC—decrease or increase by 2–4%, TAC—decrease by 3–20%, vitamin C—decrease by 15–78%), antioxidant activity (decrease or increase by 3–9%), and nephelometric turbidity (decrease or increase by 11–65%). Both nectars showed better quality and nutritional value after the HTST and UHT processes compared to treatment with classic pasteurization. Storage affected the degradation of bioactive compounds, reduced antioxidant activity, increased turbidity, and caused the brightening of samples together with reducing redness and yellowness. Considering the results obtained, it is reasonable to recommend the use of the HTST and UHT methods in industrial conditions for the preservation of liquid fruit and vegetable products such as juices, nectars, and beverages.
The Selection of Storage Period for Fresh Rice Noodles, Processing Materials, and the Screening of Key Indicators
Authors: Yang Zhe , Liu Peng , Fang Xilin , Chen Guanghui
Published: December 2024

The storage period of paddy is a critical factor affecting rice quality, and it is still unclear how fresh rice noodles, primarily made from paddy, respond to changes in the storage period. To elucidate the relationship between the paddy storage period and the quality of fresh rice noodles, this study used fourteen rice varieties as materials and set up three paddy storage periods (six months, nine months, and twelve months). It explored the quality variation patterns of fresh rice noodles processed from these paddies and analyzed the relationship between the two in conjunction with rice quality. The results indicated that fresh rice noodles produced from paddies stored for nine months exhibited superior quality compared to the other two storage periods. Grey relational analysis and correlation analysis confirmed that this was primarily attributed to changes in the gel consistency of the paddy. When the paddy was stored for nine months and the rice gel consistency was approximately 32 mm, the quality of the fresh rice noodles produced was optimal, serving as an important basis for selecting raw materials for fresh rice noodles.
Effect of Opuntia ficus-indica Extract in Pro-Healthy Chicken Patties: Physicochemical Properties and Oxidative Stability
Authors: Gonçalves Leticia A. , Lorenzo José M. , Bermúdez Roberto , Pateiro Mirian , Trindade Marco Antonio
Published: December 2024

Opuntia ficus-indica is a subtropical fruit rich in dietary fibers, carotenoids, vitamins, minerals, and polyphenols. To substitute synthetic additives, its extracts could become an interesting proposal to preserve quality while adding desirable characteristics to meat products. This study aimed to develop healthier chicken patties (with a structured animal fat replacer) added with prickly pear extract (PPE). The extract was analyzed for total phenolic content and antioxidant activity (FRAP, ABTS, DPPH, and ORAC). Four chicken patty formulations were manufactured with total replacement of animal fat by sesame oil emulsion: control, erythorbate 500 ppm, PPE 500, and PPE 750 ppm. Proximate composition and fatty acid profile were analyzed on day 1, and pH, color, and lipid oxidation on days 1, 4, 8, 12, and 16. PPE treatments showed lower TBARSs (p < 0.05) and greater pigment stability at the end of storage, corroborating its potential to delay oxidation reactions. No significant effects on chemical composition, pH, or fatty acid profile were observed (p > 0.05). Unsaturated represented 76.2% of total fatty acids. Therefore, PPE is an effective antioxidant by improving oxidative stability without promoting changes in other properties, besides adding cleaner label approaches and the use of natural ingredients to develop meat products reformulated with unsaturated oils.
An Evaluation of the Effects of Pepper (Zanthoxylum bungeanum Maxim.) Leaf Extract on the Physiochemical Properties and Water Distribution of Chinese Cured Meat (Larou) During Storage
Authors: Zhao Shengming , Li Mengke , Hei Mengran , Zhao Yanyan , Li Jingjun , Kang Zhuangli , Ma Hanjun , Xiong Guoyuan
Published: December 2024

In this study, pepper (Zanthoxylum bungeanum Maxim.) leaf (ZL) extract was added to larou to investigate the improvement in the quality of physicochemical properties, texture, water distribution, and microorganism growth during storage for 20 days. Based on the results, the addition of ZL extract significantly retarded the increase in cooking loss, TBARS value, hardness, and microorganism growth. Moreover, the addition of ZL extract decreased the pH value, lightness, and microorganism counts, and increased the moisture content, total soluble protein content, a* value, b* value, and chewiness. The LF-NMR results showed that the addition of ZL extract shortened the T2 relaxation time and boosted the proportion of immobilized water, facilitating the validation of the improvement in water retention of larou during storage. The FT-IR results indicated that the addition of ZL extract influenced the protein secondary structure by inducing the conversion of α-helices to β-sheet structures. Accordingly, ZL extract has the potential to serve as a natural antioxidant, effectively helping to ameliorate the quality properties of cured meat products during storage.
Integrated Transcriptomic and Metabolomic Analyses Reveal Changes in Aroma- and Taste-Related Substances During the Withering Process of Black Tea
Authors: Ntezimana Bernard , Xu Wenluan , Li Yuchuan , Zhou Jingtao , Pathak Sujan , Chen Yuqiong , Yu Zhi , Zhang De , Ni Dejiang
Published: December 2024

Withering is one of the major processing steps critical for the quality of black tea. In this study, we investigated the mechanisms underlying the physicochemical changes in metabolites and gene expression during the withering process of black tea using metabolomic and transcriptomic approaches, respectively. Based on gas chromatography/mass spectrometry non-targeted metabolomic approaches (GC-MS) and ultra-high performance liquid chromatograph–tandem mass spectrometry (UHPLC-MS/MS), a total of 76 volatile compounds and 160 non-volatile compounds were identified from tea leaves, respectively. RNA-seq analysis revealed that the number of differentially expressed genes (DEGs) for the comparative combination of withering time (i.e., W4h, W6h, W8h, W10h, and W12h) compared with CK (i.e., fresh leaves) were 3634, 2906, 4127, 5736, and 7650, respectively. The core genes in starch metabolism, namely alpha-amylase (AMY) and beta-amylase (BAM), were upregulated as withering time increased. AMY and BAM contributed to the decomposition of starch to increase the soluble sugars. The content of tea leaf alcohols and aldehydes, which are the vital contributors for greenish aroma, gradually decreased as withering time increased due to the downregulation of associated genes while the compounds related to sweet and fruity characteristics increased due to the upregulated expression of related genes. Most DEGs involved in amino acids were significantly upregulated, leading to the increase in free amino acids content. However, DEGs involved in catechins metabolism were generally downregulated during withering, and resulted in a reduction in catechins content and the accumulation of theaflavins. The same trend was observed in alpha-linolenic acid metabolism-related genes that were downregulated and enhanced the reduction in grassy aroma in black tea. The weighted gene co-expression network analysis (WGCNA) of DEGs showed that one module can be associated with more components and one component can be regulated by various modules. Our findings provide new insights into the quality formation of black tea during the withering process.
Non-Thermal Treatment Mediated by Curcumin for Enhancing Food Product Quality
Authors: Wang Ziyuan , Yang Haihong , Li Zhaofeng , Liu Jie
Published: December 2024

Increasing antibiotic resistance is one of the world’s greatest health problems, and biocide use in food disinfection, alongside other application fields, could increase antibiotic resistance. Effective and eco-friendly food decontamination treatment with minimal chemical intervention in food production is urgently needed. Synergistic antimicrobial interaction of photoactive compounds and blue-light-emitting diodes have recently been proven effective in agricultural and environmental applications. Curcumin-based non-thermal treatment has been reviewed in this work for the development of a safe and effective decontamination tool that could be adapted to the food industry. The antimicrobial mechanism of the synergistic interaction and the inhibitory efficacy against foodborne pathogens (bacteria in both vegetative form and spore, as well as in biofilms) are discussed. Further studies on curcumin and its derivative, as well as light illumination patterns, were compared for enhanced bactericidal efficacy. Moreover, studies relating to photodynamic inactivation treatment for food sanitation and food quality enhancement (cereal grains and other food products) were summarized, as well as the impact on food organoleptic and nutritional quality.
Composite Contrast Enhancement of Hydrogel-Based Implants for Photon-Counting Computed Tomography Studies
Authors: Suslova Evgeniya V. , Shashurin Denis A. , Maslakov Konstantin I. , Kupreenko Stepan Yu. , Luneva Tatyana O. , Medvedev Oleg S. , Chelkov Georgy A.
Published: December 2024

Hydrogels have a wide range of medical applications, including use within implantable systems. However, when used in implants, their visibility under conventional medical imaging techniques is limited, creating safety risks for patients. In the current work, we assessed the possibility of enhancing hydrogels using Ln-based contrasting agents to facilitate their visualization in photon-counting computed tomography (PCCT). The contrast enhancement of gelatin, polyacrylamide (PAM), and silicone shells of implants was assessed. A novel synthetic route for producing cross-linked nanosized Ln2O3 with polyacrylamide was proposed and discussed in detail. Several prototypes of silicone implants, including silicone shell and gelatin or PAM filling with different combinations of contrasting agents, were produced and assessed in phantom PCCT studies.
Comfort-Promoting Interventions for the Elderly in Hospital Settings
Authors: Marques Rita , Dixe Maria dos Anjos , Sousa Patrícia Pontífice
Published: December 2024

Background: The comfort of the elderly in hospital settings requires special attention from the health care professionals involved, particularly nurses, since hospitalization often generates suffering and discomfort. In such contexts, it is essential to consider the specific characteristics of the elderly, taking into account their life experiences and their needs, to promote the health, well-being, and comfort of this population. Hence, the present work aimed to explore the nursing interventions that promote comfort among the elderly in hospital settings. Methods: A mixed descriptive exploratory study was conducted through the application of a questionnaire, using intentional non-probabilistic sampling. The study encompassed 55 elderly individuals hospitalized in the medical service of a public hospital located in Lisbon. Results: The results show that the participants perceived a reasonable level of comfort (5.65 ± 6.46). The following categories emerged from the content analysis: (1) physical interventions; (2) psycho-spiritual interventions; (3) socio-cultural interventions; and (4) environmental interventions. Conclusions: These findings help to understand comfort-promoting nursing interventions in the studied population. It was concluded that, to improve care quality, comforting interventions should focus on the elderly patient’s individuality, through support activities, empowerment, and the preservation/correction of the surrounding environment. Additionally, the provided care should be based on the real needs, expectations, preferences, and values of the elderly individual.
Cost-Effectiveness of the Pneumococcal Vaccine in the Adult Population: A Systematic Review
Authors: Vo Nam Xuan , Pham Huong Lai , Bui Uyen My , Ho Han Tue , Bui Tien Thuy
Published: December 2024

Objectives: Pneumococcal disease (PD), caused by S. pneumoniae, is a serious global health issue, primarily for adults over 65, due to its high mortality and morbidity rates. Recently, broader-serotype vaccines have been introduced to cope with tremendous hospital costs and decreasing quality of life. Our study aims to systematically review the cost-effectiveness of current PCVs (pneumococcal conjugate vaccines) and PPVs (pneumococcal polysaccharide vaccine) from 2018 to April 2024. Methods: Articles were identified through PubMed, Embase, and Cochrane. Key outcomes include an improved incremental cost-effectiveness ratio (ICER) and quality-adjusted life-years (QALY), with the article’s quality assessed via the Consolidated Health Economic Evaluation Reporting Standards 2022 (CHEERS 2022). In total, 23 studies were included, with 22 studies of high quality and 1 of moderate quality. Results: These articles showed that PCV20 was the most cost-effective option compared with other vaccines, including PPV23, PCV13, PCV15, and PCV15/PPV23, for both young and older adults, regardless of risk factors. PCV20, when used alone, saved greater costs than PCV20, followed by PPV23. Conclusions: For countries applying lower-valency vaccines, switching to PCV20 as a single regimen would be the most beneficial for averting pneumococcal cases and reducing costs in adults aged 18–64 and over 65.
Identification of the LH2 Locus for Prostrate Hair Density in Grapevine
Authors: Yang Bohan , Liu Jiaqi , Gu Qinqin , Xu Zhizhuo , Yao Xiukun , Liang Jianxiang , Xu Menghao , Lu Jiang , Fu Peining
Published: December 2024

Prostrate hairs are one of the anatomic barriers for grapevine resistance to pests and diseases, as well as in environmental adaptability, making them valuable for breeding programs. This study investigates the genetic determinants underlying prostrate hair density in grapevine, a key trait associated with resistance to pests and pathogens. Using an F1 hybrid population derived from Vitis vinifera L. ‘Cabernet Sauvignon cv.’ and V. pseudoreticulata W.T.Wang ‘Huadong1058’, we performed a combination of quantitative trait locus (QTL) mapping and genome-wide association study (GWAS) to identify the genomic regions influencing the density of prostrate hair. We identified a major locus on 9.56–10.54 Mbp of chromosome 17, designated as ‘LH2’, which accounts for 43% of the phenotypic variation. This locus was delineated with high precision, and 92 candidate genes were identified within the region. Functional enrichment analysis suggested that these genes are potentially involved in binding, catalytic activity, and various cellular processes. In particular, the SNP markers ‘chr17_10130288’ and ‘chr17_10428273’ were significantly associated with prostrate hair density, providing valuable information for marker-assisted selection. These findings offer a reliable target for analyzing the hair development mechanism of grapevine leaves and breeding new cultivars rich in prostrate hair on the back of the leaves.
Using Two Water Quality Indices for Evaluating the Health and Management of a Tropical Lake
Authors: Nerae Meareg D. , Zimale Fasikaw A. , Steenhuis Tammo S. , Kebedew Mebrahtom G.
Published: December 2024

With increasing pressure on freshwater resources in developing countries due to population growth, further research and potential interventions are crucial. Lake Tana, located in the headwaters of the Blue Nile, serves as a critical example of these precious freshwater resources. This study evaluated the water quality of Lake Tana for both ecological health and drinking purposes using the Arithmetic Weighted Water Quality Index (AW WQI) and the Canadian Council of Ministers of the Environment Water Quality Index (CCME WQI). Samples were collected from 20 lake sampling stations four times between July 2018 and June 2019 to calculate the two water quality indices using ten measured parameters. The average annual AW WQI ranged from good to very poor for ecological health and very poor to unsuitable for drinking water. The CCME WQI categorized Lake Tana’s water quality as poor to fair for both uses. According to the water quality indices, the water quality was most impacted by turbidity, dissolved oxygen, ammonium, and phosphorus. However, except for ammonium, these factors were immaterial for lake management because the lake was nitrogen-limited, and the turbidity resulted from sediment stirred up by waves from the lake bottom, which cannot be managed easily. Dissolved oxygen is related to turbidity. Moreover, the WQIs did not identify two pesticides in the lake that negatively affected the fish. Thus, WQI indices may document water quality changes over time. Therefore, in addition to a favorable economic and political climate, improving lake water management requires insights from experts, the scientific literature, and possibly additional monitoring in addition to what is provided by the WQIS.
Thymol Impacts the Progression of Endometriosis by Disrupting Estrogen Signaling Pathways and Inflammatory Responses
Authors: Zhang Yu , Shaukat Aftab , Zhang Han , Yang Yao-Feng , Li Hui-Xia , Li Guang-Ya , Liu Ying-Nan , Liang Chen , Kang Jin-Wen , Li Shao-Chuan , Su Ren-Wei
Published: December 2024

Endometriosis is a chronic inflammatory, estrogenic disorder caused by endometrial tissue growth places other than uterine lumen, resulting in infertility and severe pelvic pain. Thymol, an extract of Thymus vulgaris, processes diverse biological properties, including anti-inflammatory, local anesthetic, decongestant, and antiseptic effects. However, the efficacy of thymol in treating endometriosis has still not been explored. Herein, this research aimed to investigate the role of thymol in the treatment of endometriosis using a murine model and Ishikawa cells. Thirty C57BL/6 mice were administered 17β-E2 (100 ng/mouse) subcutaneously for three consecutive days to induce synchronous estrus. On the last day of injection, the mice underwent surgical induction of endometriosis. After that, the mice were divided into three groups, i.e., Control (CTRL), Thymol 30 mg/kg and Thymol 60 mg/kg, receiving oral administration of either saline or thymol (30 mg/kg/d or 60 mg/kg/d, as 0.1 mL/kg/d, respectively) for a three-week duration. Each group consisted of ten mice and was evenly divided into estrus and diestrus according to the vaginal cytology on the last day of treatment. Thymol significantly (p < 0.05) reduced the weight and volume of ectopic tissue, hindered cell proliferation, and stimulated apoptosis compared to the CTRL group. Additionally, in the thymol-treated group, the levels of pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as well as the numbers of neutrophils and macrophages, were significantly (p < 0.05) decreased. Moreover, a novel role of thymol in rebalancing estrogen and progesterone (E2-P4) signaling was explored, and it was distributed in the ectopic endometrium. Next, the role of thymol on Ishikawa cells was determined. The results demonstrated that thymol significantly (p < 0.05) suppressed the E2-induced proliferation of Ishikawa cells. Furthermore, molecular docking analyses suggested that thymol potentially binds to ESR1-like estrogens, indicating its antagonistic activity against estrogens. The estrogen receptor 1 (ESR1) and its target gene expression exhibited significant (p < 0.05) downregulation, while progesterone receptor (PGR) and target genes were markedly (p < 0.05) upregulated following thymol treatment in the ectopic endometrium. Most importantly, our data revealed the minimal impact of thymol treatment on the eutopic endometrium and its crucial role in supporting pregnancy, thus indicating the safety of thymol in treating endometriosis. Overall, our study suggests that thymol holds promising therapeutic implications for endometriosis by virtue of its anti-inflammatory properties and ability to antagonize estrogen activity.
The Nucleocapsid (N) Proteins of Different Human Coronaviruses Demonstrate a Variable Capacity to Induce the Formation of Cytoplasmic Condensates
Authors: Tikhomirova Maria A. , Kuzmenko Oleg L. , Arifulin Eugene A. , Shirokova Olesya M. , Musinova Yana R. , Sheval Eugene V.
Published: December 2024

To date, seven human coronaviruses (HCoVs) have been identified. Four of these viruses typically manifest as a mild respiratory disease, whereas the remaining three can cause severe conditions that often result in death. The reasons for these differences remain poorly understood, but they may be related to the properties of individual viral proteins. The nucleocapsid (N) protein plays a crucial role in the packaging of viral genomic RNA and the modification of host cells during infection, in part due to its capacity to form dynamic biological condensates via liquid–liquid phase separation (LLPS). In this study, we investigated the capacity of N proteins derived from all HCoVs to form condensates when transiently expressed in cultured human cells. Some of the transfected cells were observed to contain cytoplasmic granules in which most of the N proteins were accumulated. Notably, the N proteins of SARS-CoV and SARS-CoV-2 showed a significantly reduced tendency to form cytoplasmic condensates. The condensate formation was not a consequence of overexpression of N proteins, as is typical for LLPS-inducing proteins. These condensates contained components of stress granules (SGs), indicating that the expression of N proteins caused the formation of SGs, which integrate N proteins. Thus, the N proteins of two closely related viruses, SARS-CoV and SARS-CoV-2, have the capacity to antagonize SG induction and/or assembly, in contrast to all other known HCoVs.
CD3+CD4-CD8- Double-Negative Lymphocytes Are Increased in the Aqueous Humor of Patients with Retinitis Pigmentosa: Their Possible Role in Mediating Inflammation
Authors: Bacherini Daniela , Maggi Laura , Faraldi Francesco , Sodi Andrea , Vannozzi Lorenzo , Mazzoni Alessio , Capone Manuela , Virgili Gianni , Vicini Giulio , Falsini Benedetto , Cosmi Lorenzo , Viggiano Pasquale , Rizzo Stanislao , Annunziato Francesco , Giansanti Fabrizio , Liotta Francesco
Published: December 2024

Recently, evidence has supported a significant role for immune and oxidative-mediated damage underlying the pathogenesis of different types of retinal diseases, including retinitis pigmentosa (RP). Our study aimed to evaluate the presence of immune cells and mediators in patients with RP using flow cytometric analysis of peripheral blood (PB) and aqueous humor (AH) samples. We recruited 12 patients with RP and nine controls undergoing cataract surgery. Flow cytometric analysis of PB and AH samples provided a membrane staining that targeted surface molecules (CD14, CD16, CD19, CD3, CD4, CD8, and CD161) identifying monocytes, natural killer (NK) cells, B cells, T cells, and T subpopulations, respectively. Moreover, lymphocytes were polyclonally stimulated to evaluate cytokine (CK) production at single-cell level. The circulating immune cell distribution was comparable between patients with RP and controls. Conversely, in the AH of controls we could detect no cells, while in the RP AH samples we found infiltrating leukocytes, consisting of T (CD3+), B (CD19+), NK (CD16+CD3-) cells, and monocytes (CD14+). In patients with RP, the frequency of most infiltrating immune cell populations was similar between the AH and PB. However, among T cell subpopulations, the frequency of CD3+CD4+ T cells was significantly lower in the RP AH compared to RP PB, whereas CD3+CD4-CD8- double-negative (DN) T cells were significantly higher in the RP AH compared to RP PB. Cytokine production analysis revealed a trend toward an increased frequency of CD3+CD8-CD161+IFN-ɣ-producing cells and a decreased frequency of CD3+CD8+IL-4-producing cells in the RP AH compared to RP PB. The detection of immune cells, particularly DN T cells, and a Th1-skewed phenotype in RP AH suggests immune-mediated and inflammatory mechanisms in the disease.
Molecular Pathway and Immune Profile Analysis of IPMN-Derived Versus PanIN-Derived Pancreatic Ductal Adenocarcinomas
Authors: Park Margaret A. , Gumpper-Fedus Kristyn , Krishna Somashekar G. , Genilo-Delgado Maria C. , Brantley Stephen , Hart Phil A. , Dillhoff Mary E. , Gomez Maria F. , Basinski Toni L. , Mok Shaffer R. , Luthra Anjuli K. , Fleming Jason B. , Mohammadi Amir , Centeno Barbara A. , Jiang Kun , Karolak Aleksandra , Jeong Daniel , Chen Dung-Tsa , Stewart Paul A. , Teer Jamie K. , Cruz-Monserrate Zobeida , Permuth Jennifer B.
Published: December 2024

Intraductal papillary mucinous neoplasms (IPMN) are commonly detected pancreatic cysts that may transform into pancreatic ductal adenocarcinoma (PDAC). Predicting which IPMNs will progress to PDAC remains a clinical challenge. Moreover, identifying those clinically evident IPMNs for which a surveillance approach is best is a dire clinical need. Therefore, we aimed to identify molecular signatures that distinguished between PDAC with and without clinical evidence of an IPMN to identify novel molecular pathways related to IPMN-derived PDAC that could help guide biomarker development. Data from the Oncology Research Information Exchange Network (ORIEN) multi-institute sequencing project were utilized to analyze 66 PDAC cases from Moffitt Cancer Center and The Ohio State University Wexner Medical Center, for which tumor whole transcriptome sequencing datasets were generated. Cases were classified based on whether a tumor had originated from an IPMN (n = 16) or presumably through the pancreatic intraepithelial neoplasia (PanIN) pathway (n = 50). We then performed differential expression and pathway analysis using Gene-Set Enrichment Analysis (GSEA) and Pathway Analysis with Down-weighted Genes (PADOG) algorithms. We also analyzed immune profiles using the Tumor-Immune Microenvironment Deconvolution web portal for Bulk Transcriptomics (TIMEx). Both GSEA and TIMEx indicate that PanIN-derived PDAC tumors enrich inflammatory pathways (complement, hedgehog signaling, coagulation, inflammatory response, apical surface, IL-2/STAT5, IL-6/STAT3, EMT, KRAS signaling, apical junction, IFN-gamma, allograft rejection) and are comparatively richer in almost all immune cell types than those from IPMN-derived PDAC. IPMN-derived tumors were enriched for metabolic and energy-generating pathways (oxidative phosphorylation, unfolded protein response, pancreas beta cells, adipogenesis, fatty acid metabolism, protein secretion), and the most significantly upregulated genes (padj < 0.001) included mucin 2 (MUC2) and gastrokine-2 (GKN2). Further, the metabolic-linked gene signature enriched in the IPMN-derived samples is associated with a cluster of early-stage and long-survival (top 4th quartile) PDAC cases from The Cancer Genome Atlas (TCGA) expression database. Our data suggest that IPMN-derived and PanIN-derived PDACs differ in the expression of immune profiles and metabolic pathways. These initial findings warrant validation and follow-up to develop biomarker-based strategies for early PDAC detection and treatment.