bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of p38α signaling networks in cancer cells using quantitative proteomics and phosphoproteomics
Authors: Dan Yuzhen , Radic Nevenka , Gay Marina , Fernández-Torras Adrià , Arauz Gianluca , Vilaseca Marta , Aloy Patrick , Canovas Begoña , Nebreda Angel R.
Published: September 2022

p38α (encoded by MAPK14) is a protein kinase that regulates cellular responses to almost all types of environmental and intracellular stresses. Upon activation, p38α phosphorylates many substrates both in the cytoplasm and nucleus, allowing this pathway to regulate a wide variety of cellular processes. While the role of p38α in the stress response has been widely investigated, its implication in cell homeostasis is less understood. To investigate the signaling networks regulated by p38α in normally proliferating cancer cells, we performed quantitative proteomic and phosphoproteomic analyses in breast cancer cells in which this pathway had been either genetically targeted or chemically inhibited. Our study identified with high confidence 35 proteins and 82 phosphoproteins (114 phosphosites) that are modulated by p38α, and highlighted the implication of various protein kinases, including MK2 and mTOR, in the p38α-regulated signaling networks. Moreover, functional analyses revealed an important contribution of p38α to the regulation of cell adhesion, DNA replication and RNA metabolism. Indeed, we provide experimental evidence supporting that p38α negatively regulates cell adhesion, and showed that this p38α function is likely mediated by the modulation of the adaptor protein ArgBP2. Collectively, our results illustrate the complexity of the p38α regulated signaling networks, provide valuable information on p38α-dependent phosphorylation events in cancer cells, and document a mechanism by which p38α can regulate cell adhesion.
Anterior hypothalamic parvalbumin neurons are glutamatergic and promote escape behavior
Authors: Laing Brenton T. , Anderson Megan S. , Bonaventura Jordi , Jayan Aishwarya , Sarsfield Sarah , Gajendiran Anjali , Michaelides Michael , Aponte Yeka
Published: September 2022

The anterior hypothalamic area (AHA) is a critical structure for defensive responding. Here, we identified a cluster of parvalbumin-expressing neurons in the AHA (AHAPV) that are glutamatergic with fast-spiking properties and send axonal projections to the dorsal premammillary nucleus (PMD). Using in vivo functional imaging, optogenetics, and behavioral assays, we determined the role of these AHAPV neurons in regulating behaviors essential for survival. We observed that AHAPV neuronal activity significantly increases when mice are exposed to a predator, and in a real-time place preference assay, we found that AHAPV neuron photoactivation is aversive. Moreover, activation of both AHAPV neurons and the AHAPV→PMD pathway triggers escape responding during a predator-looming test. Furthermore, escape responding is impaired after AHAPV neuron ablation, and anxiety-like behavior as measured by the open field and elevated plus maze assays does not seem to be affected by AHAPV neuron ablation. Finally, whole-brain metabolic mapping using positron emission tomography combined with AHAPV neuron photoactivation revealed discrete activation of downstream areas involved in arousal, affective, and defensive behaviors including the amygdala and the substantia nigra. Our results indicate that AHAPV neurons are a functional glutamatergic circuit element mediating defensive behaviors, expanding the identity of genetically defined neurons orchestrating fight-or-flight responses. Together, our work will serve as a foundation for understanding neuropsychiatric disorders such as aggression or fear.
Staphyloxanthin production by Staphylococcus aureus promotes resistance to oxidative stress to delay diabetic wound healing
Authors: McCready-Vangi Amelia R. , Campbell Amy E. , Uberoi Aayushi , Lovins Victoria M. , White Ellen K. , Morgenstern Alexis R. , Gardner Sue E. , Grice Elizabeth A.
Published: September 2022

Diabetic foot ulcers (DFU) are a serious complication of diabetes mellitus that burden patients and health care systems. Staphylococcus aureus is prevalent and abundant in the DFU microbiome, and strain-level differences in S. aureus may drive clinical outcomes. To identify mechanisms underlying strain-specific outcomes in DFU with S. aureus, we performed high-throughput phenotyping screens on a collection of 221 S. aureus cultured isolates from clinically uninfected DFU. Of the 4 phenotypes examined (in vitro biofilm formation and production of staphylokinase, staphyloxanthin, and siderophores), we discovered that isolates from non-healing wounds produced more staphyloxanthin, a carotenoid cell membrane pigment. In a murine diabetic wound healing model, staphyloxanthin-producing isolates delayed wound closure significantly compared to staphyloxanthin-deficient isolates. Staphyloxanthin promoted resistance to oxidative stress in vitro and enhanced bacterial survival in human neutrophils. Comparative genomic and transcriptomic analysis of genetically similar clinical isolates with disparate staphyloxanthin phenotypes revealed a mutation in the Sigma B regulatory pathway that resulted in marked differences in stress response gene expression. Our findings suggest that staphyloxanthin production delays wound healing by protecting S. aureus from neutrophil-mediated oxidative stress, and may provide a target for therapeutic intervention in S. aureus-positive wounds.
Ribosomes as molecular thermometers: metal-binding sites in ribosomal proteins are robust indicators of bacterial adaptation to heat and cold
Authors: van den Elzen Antonia , Helena-Bueno Karla , Brown Charlotte R. , Melnikov Sergey
Published: September 2022

Abstract

Ribosomal genes are widely used as “molecular clocks” to infer the evolutionary relatedness of species. It is unclear, however, whether these genes can also serve as “molecular thermometers” to precisely estimate an organism’s optimal growth temperature. Previously, some estimations were made using the average nucleotide content in ribosomal RNA, but the universal application of this approach was prevented by numerous outliers. Here, seeking to bypass this problem, we asked whether ribosomal genes contain additional markers of thermal adaptations, aside from their nucleotide composition. To answer this, we analyzed site-specific variations in sequences of ribosomal proteins from 2,021 bacteria with known optimal growth conditions. We found that ribosomal proteins comprise a few “mutational hotspots”—residues that vary in a temperature-dependent manner and distinguish heat- and cold-adapted bacteria. Most of these residues coordinate metal ions that support protein folding at high temperatures. Using these residues, we then showed that the upper and lower limits of an organism’s optimal growth temperatures can be estimated using just 0.001% of the genome sequence or just two amino residues in the cellular proteome. This finding illustrates that laboratory-independent estimation of optimal growth temperatures can be simplified if we abandon the traditional use of rRNA and protein sequences to assess their content and instead focus on those few residues that are most critical for protein structure. This finding may simplify the analysis of unculturable and extinct species by helping bypass the need for laborious, costly, and at times impossible laboratory experiments.
Serum-dependent recruitment of the chromatin remodeler CHD8 to promoters is mediated by the ERK-ELK pathway
Authors: Subtil-Rodríguez Alicia , Vázquez-Chávez Elena , Guerrero-Martínez Jose A. , Ceballos-Chávez María , Reyes José C.
Published: September 2022

Chromodomain helicase DNA binding protein 8 (CHD8) is a chromatin remodeler of the SNF2 family involved in gene transcription regulation. It has been shown that CHD8 is required for cell proliferation, cell differentiation and central nervous system development. In fact, CHD8 haploinsufficiency causes a human syndrome characterized by autism, macrocephaly, gastrointestinal complaints and some other clinical characteristics. However, the mechanism by which CHD8 controls transcription and how it is recruited to its targets in the chromatin is still unclear. We have previously shown that serum depletion causes that CHD8 detaches from chromatin. Here we demonstrate that serum-dependent recruitment of CHD8 to promoters requires the extracellular signal-regulated kinase (ERK)/ ETS-like (ELK) branch of the mitogen-activated protein kinase (MAPK) pathway. Our analysis of genomic occupancy data shows that CHD8 binding sites were strongly enriched in ELK1 and ELK4 DNA binding motifs and that CHD8 and ELK1 co-occupy multiple transcription start sites. We show that ELK1 and ELK4 are required for normal recruitment of CHD8 to the promoters of CCNA2, CDC6, CCNE2, BRCA2 and MYC genes. However, CHD8 is dispensable for ELK1 and ELK4 binding. Genome wide transcriptomic analysis evidenced that serum-dependent activation of a subset of immediate early genes, including the well-known ELK1 target gene FOS, was impaired upon depletion of CHD8. In summary, our results uncover the role of the ERK/ELK pathway in CHD8 recruitment to chromatin and provide evidences indicating a role of CHD8 in regulating serum-dependent transcription.
Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo
Authors: Lisitskaya Lidiya , Kropocheva Ekaterina , Agapov Aleksei , Prostova Maria , Panteleev Vladimir , Yudin Denis , Ryazansky Sergey , Kuzmenko Anton , Aravin Alexei A. , Esyunina Daria , Kulbachinskiy Andrey
Published: September 2022

Prokaryotic Argonaute proteins (pAgos) are homologs of eukaryotic Argonautes (eAgos) that were similarly proposed to play a role in cell defense against invaders. However, pAgos are much more diverse than eAgos and very little is known about their functional activity and target specificity in vivo. Here, we describe five pAgo proteins from mesophilic bacteria that act as DNA-guided DNA endonucleases and analyze their ability to target chromosomal and invader DNA. In vitro, the analyzed proteins use small guide DNAs for precise cleavage of single-stranded DNA at a wide range of temperatures. Upon their expression in Escherichia coli, all five pAgos are loaded with small DNAs preferentially produced from plasmid DNA and from chromosomal regions of replication termination. One of the tested pAgos, EmaAgo from Exiguobacterium marinum can induce DNA interference between multicopy sequences resulting in targeted processing of homologous plasmid and chromosomal loci. EmaAgo also protects bacteria from bacteriophage infection and is preferentially loaded with phage guide DNAs suggesting that the ability of pAgos to target multicopy elements may be crucial for their protective function. The wide spectrum of pAgo activities suggests that they may have diverse functions in vivo and paves the way for their use in biotechnology.
Expression profiling of immune-response genes common in SARS-CoV-1 and influenza A virus disease, viz-a-viz neutropenia disorder, using integrated bioinformatics tools
Authors: Olaniyi Ayinde , Oluwadamilola Obasanmi , Oluwabunmi Alabi
Published: September 2022

Viral diseases have proven to be an existential threat to man due to its fatality and its consistent emergence and re-emergence, so the need for novel ideas in combating this menace. Advances in genetic studies has proven to be indispensable in this fight as knowledge of organismal genetic variability has been useful in therapy development, as well as in mounting defense against the treacherous infectious agents. However, there are still fallow grounds needing to be explored in this war which informed this study, with focus on expression profiling of similar immune-responsegenes in SARS-COV-1 and influenza A virus (AIV) in respect to neutropenia (NP), using integrated bioinformatics techniques such as extraction of microarray dataset in the gene expression omnibus (GEO) database, generation of DEGs using GEO2R tool, construction of protein-protein interaction (PPI) network using string and Cytoscape software, Venn diagram analysis and then gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) for enrichment and pathway analysis respectively. Ten genes which includes ELANE, ITGA2B, CXCR1, CSF3R, SPI1, MS4A3, MMP8, CEACAM8, RNASE3, and DEFA4 were identified, with ELANE gene moreover identified as a key gene, which might be responsible in the regulation of immune response during viral infection, based on its characteristic feature in the PPI network.
A new and highly effective method for predicting T-cell response targets implemented on SARS-CoV-2 data
Authors: Flegr Jaroslav , Zahradník Daniel
Published: September 2022

Computational T-cell epitope prediction is essential in many immunological projects, including the development of vaccines. T-cells of immunocompetent vertebrate hosts can recognize as non-self only peptides which are present in the parasite’s proteins and absent in the host’s proteins. This basic principle allows us to predict which peptides can elicit T-cells’ response. We built on the fact that the specificity of T-cells reacting to SARS-CoV-2 antigens has been recently mapped in detail. Using Monte Carlo tests, we found that empirically confirmed peptides that stimulate T-cells contain an increased fraction of pentapeptides, hexapeptides, and heptapeptides which are not found in the human proteome (p < 0.0001). Similarly, hexapeptides absent in human proteins were overrepresented in peptides that elicited T-cell response in a published empirical study (p = 0.027). The new theory-based method predicted T-cell immunogenicity of SARS-CoV-2 peptides four times more effectively than current empirically based methods.
Genomic features predict bacterial life history strategies in soil, as identified by metagenomic stable isotope probing
Authors: Barnett Samuel E. , Egan Rob , Foster Brian , Eloe-Fadrosh Emiley A. , Buckley Daniel H.
Published: September 2022

Bacteria catalyze the formation and destruction of soil organic matter, but the bacterial dynamics in soil that govern carbon (C) cycling are not well understood. Life history strategies explain the complex dynamics of bacterial populations and activities based on tradeoffs in energy allocation to growth, resource acquisition, and survival. Such tradeoffs influence the fate of soil C, but their genomic basis remains poorly characterized. We used multi-substrate metagenomic DNA stable isotope probing to link genomic features of bacteria to their C acquisition and growth dynamics. We identify several genomic features associated with patterns of bacterial C acquisition and growth, notably genomic investment in resource acquisition and regulatory flexibility. Moreover, we identify genomic tradeoffs defined by numbers of transcription factors, membrane transporters, and secreted products, which match predictions from life history theory. We further show that genomic investment in resource acquisition and regulatory flexibility can predict bacterial ecological strategies in soil.
Identification of regulatory elements in primary sensory neurons involved in neuropathic pain
Authors: Stephens Kimberly E. , Moore Cedric , Vinson David A. , White Bryan E. , Renfro Zachary , Zhou Weiqiang , Ji Zhicheng , Ji Hongkai , Zhu Heng , Guan Yun , Taverna Sean D.
Published: September 2022

Chronic pain is a significant public health issue that is often refractory to existing therapies. Here we use a multiomic approach to identify cis-regulatory elements that show differential chromatin accessibility, and reveal transcription factor (TF) binding motifs with functional regulation in the dorsal root ganglion (DRG), which contain cell bodies of primary sensory neurons, after nerve injury. We integrated RNA-seq to understand how differential chromatin accessibility after nerve injury may influence gene expression. Using TF protein arrays and chromatin immunoprecipitation-qPCR, we confirmed C/EBPγ binding to a differentially accessible sequence and used RNA-seq to identify processes in which C/EBPγ plays an important role. Our findings offer insights into TF motifs that are associated with chronic pain. These data show how interactions between chromatin landscapes and TF expression patterns may work together to determine gene expression programs in DRG neurons after nerve injury.
Tethered agonist activated ADGRF1 structure reveals molecular preference for Gαq signalling
Authors: Jones Daniel T. D. , Dates Andrew N. , Rawson Shaun D. , Burruss Maggie M. , Lipper Colin H. , Blacklow Stephen C.
Published: September 2022

Adhesion G-Protein Coupled Receptors (aGPCRs) have evolved an activation mechanism to translate extracellular force into liberation of a tethered agonist (TA) to modulate cell signalling. We report here that ADGRF1 is the first class B GPCR shown to signal through all major G-protein classes and identify the structural basis for its Gαq preference by cryo-EM. Our structure shows that Gαq over Gαs preference in ADGRF1 derives from tighter packing at the conserved F569 of the TA, altering contacts between TM helix I and VII, with a concurrent rearrangement of TM helices VII and VIII at the site of Gα recruitment. Gαs signalling is also more sensitive to mutation of TA or binding site residues than Gαq. Our work advances the understanding of aGPCR TA activation in molecular detail, identifying structural features that potentially explain preferential signal modulation.
The effect of transformational, transactional and authentic leadership on innovation: the mediating role of organizational culture
Authors: Rashwan Kareem A. , Ghaly Meril
Published: September 2022

Purpose

This study aims to investigate the effects of different leadership styles, transformational, transactional and authentic leadership, on travel agents’ innovational behavior, with organizational culture as a mediator.

Design/methodology/approach

The study aim was achieved by using an integrative model using LISREL 8.80. Quantitative research was used using a questionnaire. A convenient sampling technique was used to select the sample. A total of 500 questionnaires were distributed to travel agents, out of which 415 were returned, and 376 usable ones were statistically handled (75.2% response rate).

Findings

Transformational leadership has a significant impact on organizational culture (ß = 0.34) and innovational behavior (ß = 0.46). Authentic leadership has a significant impact on both organizational culture (ß = 0.38) and innovational behavior (ß = 0.52). Transactional leadership significantly impacts organizational culture (ß = 0.38); however, it has no significant impact on innovational behavior (ß = 0.31). The positive influence of leadership styles on innovative behavior is mediated by innovative organizational culture (ß = 0.92).

Research limitations/implications

Further research should explore the relationship between other leadership styles and innovation. Additionally, investigates the relationship between organizational culture factors and innovation. This study targeted category “A” travel agencies. Therefore, to generalize the study findings, the study should be replicated with other categories of travel agencies.

Practical implications

Travel agencies organizations should invest in managers who adopt authentic and transformational leadership, whereas transactional leaders are urged to be open to new ideas, invest in the knowledge and skills of their employees, encourage friendly communication with their employees and ensure that there are reasonable punishment and reward systems within the organization.

Originality/value

This study presents new insights into tourism field by integrating the study variables (organizational culture, innovative behavior and leadership style), into one framework, particularly in developing countries’ contexts.
Losing the benefits of work passion? The implications of low ego-resilience for passionate workers
Authors: Hochwarter Wayne , Jordan Samantha L. , Fontes-Comber Ashlee , De La Haye D.C. , Khan Abdul Karim , Babalola Mayowa , Franczak Jennifer
Published: September 2022

Purpose

This research assessed the interactive effects of employee passion and ego-resilience (ER) on relevant work outcomes, including job satisfaction, citizenship behavior, job tension, and emotional exhaustion. The authors hypothesize that higher work passion is associated with less positive work outcomes when employees are low in ER.

Design/methodology/approach

The authors collected data from three unique samples (N's = 175, 141, 164) to evaluate the moderating effect across outcomes. The authors conducted analyses with and without demographic controls and affectivity (e.g. negative and positive). The authors used a time-separated data collection approach in Sample 3. The authors also empirically assess the potential for non-linear passion and ER main effect relationships to emerge.

Findings

Findings across samples confirm that high passion employees with elevated levels of ER report positive attitudinal, behavioral, and well-being outcomes. Conversely, high passion employees do not experience comparable effects when reporting low levels of ER. Results were broadly consistent when considering demographics and affectivity.

Research limitations/implications

Despite the single-source nature of the three data collections, The authors took steps to minimize common method bias concerns (e.g. time separation and including affectivity). Future research will benefit from multiple data sources collected longitudinally and examining a more comprehensive range of occupational contexts.

Practical implications

Passion is something that organizations want in all employees. However, the authors' results show that passion may not be enough to lead to favorable outcomes without considering factors that support its efficacy. Also, results show that moderate levels of passion may offer little benefit compared to low levels and may be detrimental.

Originality/value

As a focal research topic, work passion research is still in early development. Studies exploring factors that support or derail expected favorable effects of work passion are needed to establish a foundation for subsequent analyses. Moreover, the authors comment on the assumed “more is better” phenomenon. The authors argue for reconsidering the linear approach to predicting behavior in science and practice.
Future perspective of socioemotional wealth (SEW) in family businesses
Authors: Smajić Hamza , Palalić Ramo , Ahmad Nisar
Published: September 2022

Purpose

The study provides the emergence and evolution of the socioemotional wealth (SEW) concept in the family business field from 2007 (the inception date) until 2021. To provide a better overview of this notion, the study unfolds a deeper understanding of this term throughout the systematic literature review (SLR).

Design/methodology/approach

The study applies a systematic literature review (SLR) by analyzing the sample of 185SEW articles extracted from the Scopus database. To identify all relevant studies, the article selection process was carefully designed and divided into two phases with clear steps: identification of studies via databases and identification of studies via previous studies' reference lists. Selected studies were analyzed using the Bibliometrix R-tool, resulting in an analysis of the evolution of the trends in the SEW literature, citation analysis, and network analysis. Finally, this SLR included the content analysis of the 25 most-cited SEW articles.

Findings

The study provides a relevant and comprehensive overview facilitating empirical and theoretical research in this field and paving the way to develop new themes. The bottom line of the important findings is that the SEW concept is relatively new, alluding to a wealthy venue for future works. Other results and implications are discussed on the family business and SEW theme. Additionally, the study provides suggestions which could be used for future works in this area.

Originality/value

This is the first article related to the SEW concept in the family business. It portrays a clear picture of this field, providing relevant information on what has been done, as well as what the future possibilities are that might bode the future horizons in family businesses.
The challenge to survive: trauma, violence and identity in the lived experience of homeless women
Authors: Price Holly , Glorney Emily
Published: September 2022

Purpose

To understand how females who had recently been street homeless made sense of their lived experience, seven women engaged in semi-structured interviews. This study aims to provide an insight into the complexities of the gendered homeless experience, while using theories of trauma and victimisation to propose a new approach to understanding the cycle of female homelessness.

Design/methodology/approach

An interpretative phenomenological analysis approach was chosen to explore the phenomenon of female homelessness. Semi-structured interviews were conducted with a small homogenous sample of women recruited in a city in the south of England.

Findings

Two super-ordinate themes emerged: victimisation and trauma and the group and the individual. In the male-dominated world of homelessness, women were caught in a cycle of multiple traumatic loss, compounded by pervasive gender-based violence, struggles in identity and systematic control. Gendered, trauma-informed women’s homelessness services are required.

Practical implications

Findings demonstrate the desperate need for an expansion in female-only homelessness services. The lived experience of the participants adds to an evidence base, which is vital to inform effective trauma-informed gendered service provision.

Originality/value

Homelessness policies draw principally on the prevalent literature on men; the UK research with women is lacking. This study gives voice to a hidden population, using the lived experience of women to suggest a new model of female homelessness.
Provisions on frivolous claims in the European Union – Vietnam investment protection agreement – would they be benefits to Vietnam?
Authors: Nguyen Tho Thi Anh
Published: September 2022

Purpose

This paper aims to examine two issues: whether provisions on frivolous claims in the European Union (EU)–Vietnam Investment Protection Agreement (EVIPA) would be Vietnam’s intrinsic demand, and to what extent, Vietnam may enjoy the benefits from these provisions.

Design/methodology/approach

This paper combines both doctrinal legal analysis and policy research. It offers an in-depth case study of the provisions on frivolous claims in the EVIPA, compares them with those of other existing international investment agreements and arbitrations rules, examines how similar provisions in these instruments are interpreted in available practical international investment disputes, uncovers the Vietnam’s position through interviewing Vietnamese senior experts, who were members of the Vietnamese delegation negotiating the EVIPA, and through available collected data and then evaluates whether these provisions may be favourable to this country.

Findings

While the new investor-state dispute settlement (ISDS) mechanism in the EVIPA can be viewed as explicit evidence of the EU’s achievement, it may also be Vietnam’s benefits to entertain new ISDS provisions on frivolous claims. They were drafted, based on the ISDS arbitration practice, states’ experience and actual situations in Vietnam. These novel provisions, among other things, serve as Vietnam’s prerequisites to consider whether to accept the new two-tier standing mechanism or not. The inclusion of such ISDS provisions in the EVIPA, therefore, is supposed to meet the Vietnam’s intrinsic demands for defending against unfounded frivolous cases.

Originality/value

This is the first time the EU concluded an investment treaty containing innovative ISDS provisions with a developing country. This paper therefore may help envisage Vietnam’s perspective during its negotiation of provisions on frivolous claims in the EVIPA and prove that the avails of these provisions to a frequent respondent State like Vietnam can be realised. The paper’s findings mean for research in investment law as well as for policymakers as far as the frivolous cases are concerned.
The bane of P2P lending: credit scoring governance on the ASEAN fintech triumvirate
Authors: Rosdini Dini , Wahyuni Ersa Tri , Sari Prima Yusi
Published: September 2022

Purpose

This study aims to explore credit scoring regulations, governance, variables and methods used by peer-to-peer (P2P) lending platforms in key players of the Association of Southeast Asian Nations (ASEAN) region’s P2P, Indonesia, Malaysia and Singapore.

Design/methodology/approach

This study explores the P2P Lending characteristics of the three countries using qualitative literature review, interview, focus group discussion and desk research.

Findings

This study concludes that the credit scoring variables used by the countries’ companies are almost the same. Key drivers of the differences are countries’ regulations, management/business core value and credit scoring data processing methods.

Practical implications

Ultimately, this research provides a comprehensive view for investors, businesses and researchers on the topic of ASEAN credit scoring governance and will help them navigate the complexities and improve their awareness on the importance of credit scoring governance in P2P lending companies.

Originality/value

This research provides an in-depth perspective on how P2P lending companies, credit scoring governance and regulations in the biggest three countries in Southeast Asia.
A strategic framework to analyse the East Asian miracle within triple helix model – lessons for Kuwait
Authors: Arman Husam , Al-Qudsi Sulayman
Published: September 2022

Purpose

This paper aims to propose a framework that combines the triple helix model with competitive strategies concepts to capture and guide any innovation-led national development strategies.

Design/methodology/approach

This paper adopted a methodological framework based on existing methods and guidelines, the most commonly reported approach for developing a methodological framework. The review of fundamental approaches to achieving fast and sustained economic development, triple helix model and competitive strategies helped develop the methodological framework. The framework was validated and tested using the case studies approach on Korea, Taiwan and Singapore.

Findings

Kuwait aims to create an innovative environment to benefit from the innovation strategies anchored by the East Asian miracle economies and how they used the triple helix actors at different developmental stages. First, Kuwait’s research institutes and universities need to design interactive programs and activities with industry and community to help innovate solutions to current and prospective challenges. Second, the government needs to provide a competitive business environment and effective policies. Thirdly, the Kuwait industry must be encouraged to innovate and infuse modern technology practices.

Originality/value

Developing countries are trying to use science, technology and innovation as an effective strategy for achieving sustained economic growth. However, since each country has its unique conditions, learning from other success stories proved difficult if not structured in a framework designed to serve a specific purpose such as the one the authors propose in this paper.
Transformational health-care leaders in collaborative entrepreneurial model to achieve UNSDG: a qualitative study
Authors: Borde Prashant Sunil , Arora Ridhi , Kakoty Sanjeeb
Published: September 2022

Purpose

The agglomeration of specialist health-care facilities has often been restricted to metropolitan areas. This study aims to understand how health-care professionals with transformational leadership behaviors and entrepreneurial aims with a similar vision and expertise play pertinent roles in providing essential specialized health care in rural and semi-urban areas and achieving the United Nations Sustainable Development Goals (UNSDGs).

Design/methodology/approach

Qualitative synthesis using focused-group discussions and interviews was conducted in a phased manner. For this, this study has used stakeholder-theory, and dynamic-capabilities approaches.

Findings

This study explores the intricacies of collaborative entrepreneurship (CE)-based health-care ventures in developing regions and reveals five pertinent attributes: strategic control, synergy, commitment, empathy and satisfaction. This study recommends that entrepreneurial collaboration, especially by transformational health-care leaders, can significantly contribute to creating an endogenous health-care ecosystem with advanced facilities and technology-enabled modern infrastructure and augmenting regional development.

Research limitations/implications

This study was conducted in semi-urban settings in India. Future research should include other sectors and regions to generalize the findings.

Practical implications

This study benefits health-care professionals having an analogous vision, skills and entrepreneurial aims.

Social implications

Collaboration of health-care professionals and using transformational leadership behaviors can considerably contribute to providing specialist health care in developing areas and enhance patient satisfaction.

Originality/value

To the best of the authors’ knowledge, this is the first study to discuss the importance of CE in health care in developing areas. In addition, it discusses the benefits of the CE model in achieving the UNSDGs and offers valuable suggestions for health-care professionals and administrators.
The choice and acquisition of external knowledge in startups: matching improvisation to strategic flexibility
Authors: Song Jianmin , Wen Shouxun , Ren Qingzhong , Zhang Lu
Published: September 2022

Purpose

Although knowledge has become a decisive factor in the development of firms, there has been no detailed investigation into how start-ups acquire external knowledge. In order to narrow the research gap, this paper attempts to explore the mechanism of acquiring external knowledge in start-ups from the perspective of “environment–[sic.] structure” interaction.

Design/methodology/approach

This research develops a conceptual model regarding improvisation as an independent variable, strategic flexibility as a mediator, knowledge acquisition as the dependent variable and environmental mutation as a moderator between improvisation and strategic flexibility. Furthermore, this study collects the survey data from 277 firms and uses structural equation modeling (SEM) to empirically test the model and hypotheses.

Findings

The results show that creativity-bricolage and spontaneity-persistence have significantly positive effects on both capability flexibility and coordination flexibility. However, the positive effects of pressure-stress on capability flexibility and coordination flexibility are not supported. Meanwhile, the mediating roles of capability flexibility and coordination flexibility are supported. Finally, environmental mutation only positively moderates the relationship between creativity-bricolage and capability flexibility.

Originality/value

Improvisation can be seen as a core antecedent for start-ups to acquire external knowledge in environmental mutation. More specifically, the significant mediator is strategic flexibility to promote the relationship between improvisation and knowledge acquisition. The findings provide practical inspiration for start-ups to effectively utilize improvisation in emergencies.
Investigation of the critical success factors in the implementation of the lean industry 4.0 in manufacturing supply chain: an ISM approach
Authors: Kashyap Abhishek , Yadav Amarendra Kumar , Vatsa Omkar Nandan , Chandaka Trivedh Naidu , Shukla Om Ji
Published: September 2022

Purpose

The purpose of this paper is to develop an interpretive structural modeling (ISM) model to investigate the critical success factors (CSF) and the extent of CSF's influence in the implementation of lean industry 4.0 in manufacturing supply chain.

Design/methodology/approach

The study has been carried out with the help of the latest literature followed by brainstorming sessions with experts. The experts were the managers from the industries, assistant professors, and research scholars from academia working in this domain. Finally, a structured model is formed using ISM methodology for the analysis of the CSFs followed by matrice d'impacts croisés multiplication appliquée á un classment (MIAMAC) Analysis for the validation of the model.

Findings

The study identifies robotics, virtual and augmented reality and cloud computing as the main CSFs which are responsible to drive all the identified CSFs. However the CSF professional training and development (PTD) has been identified as the weakest driver but having the highest dependent power.

Research limitations/implications

The study has included nine CSFs and the contextual relationships between the CSFs are based on the knowledge and experience of the experts, which may be biased. Moreover, the paper has covered the ISM approach, and the same thing can be validated using the fuzzy-ISM and other multi-criteria decision-making (MCDM) techniques.

Originality/value

This investigation of the CSFs in the lean industry 4.0 is original and the identified CSFs are the result of the literature reviews and an extensive discussion from the experts. The paper uses the complete experience of the respective experts to make this work more effective and original.
Assessing the roles of foreign knowledge acquisition and absorptive capacity in the relationship between market orientation, innovativeness and performance
Authors: Boateng Henry , Sigdel Subodh Sharma , Ofori Kwame Simpe , Agbemabiese George Cudjoe , Hinson Robert Ebo
Published: September 2022

Purpose

This study aims to examine the effect of market orientation on foreign knowledge acquisition. It also assesses the moderating role of absorptive capacity in the relationship between foreign knowledge acquisition, firm innovativeness and performance.

Design/methodology/approach

A questionnaire was administered to 257 respondents who were owners, managers or owner/managers of export firms in Ghana. The data were analyzed with structural equation modeling.

Findings

The findings show that customer orientation significantly influenced foreign business knowledge acquisition but not performance, and that competitor orientation significantly influenced both foreign business knowledge acquisition and firm innovativeness. Absorptive capacity was found to play a significant role in linking foreign business knowledge to innovativeness.

Originality/value

The study adds to the literature on knowledge management in firms by identifying the moderating role of absorptive capacity in the relationship between foreign business knowledge acquisition, innovativeness and performance.
“My roots are green”: a phenomenological discourse on intergenerational green motherhood in non-Western consumption contexts
Authors: Ansari Nadia Umair , Rashidi Muhammad Zaki , Mehmood Kashif
Published: September 2022

Purpose

This paper aims to describe the lived experiences of modern urban mothers in Pakistan as they navigate shared motherhood responsibilities with family elders. This paper brings to light their feelings, fears and ambitions towards safeguarding the environment for the future of their children, by reconciling ancient traditions of their female elders with contemporary sustainable consumption practices.

Design/methodology/approach

This phenomenological study explores lived experiences of urban mothers through in-depth personal interviews. Their discourse explores sharing childcare responsibilities with family matriarchs, negotiating “green” parenting strategies between intergenerational parenting partners.

Findings

Navigating life through the intersections of modernity and tradition and ethical choices and consumerism, urban mothers integrate wisdom of their ancestors into their modern lives to mitigate the environmental degradation of today.

Originality/value

This study sheds light on a unique genre of green mothers, termed as the traditionally green eco-mom, which allows modern mothers and their female elders to synchronously adopt sustainable childcare behaviours that overcome intergenerational barriers by reconciling contemporary lifestyles with traditional wisdom.
Decoding momentum returns: an integrated bibliometric and content analysis approach
Authors: Joshipura Mayank , Wats Sangeeta
Published: September 2022

Purpose

Over the past three decades, numerous conceptual and empirical studies have discussed momentum investment strategies’ presence, pervasiveness and persistence. However, science mapping in the field is inadequate. Hence, this study aims to comprehend and explore current dynamics, understand knowledge progression, elicit trends through thematic map analysis, synthesize knowledge structures and provide future research directions in this domain.

Design/methodology/approach

The study applies bibliometric analysis on 562 Scopus indexed articles from 1986 to 2021. Biblioshiny version 3.1.4, a Web-based application included in Bibiliometrix package developed in R-language (Aria and Cuccurullo, 2017), was used to examine: the most prominent articles, journals, authors, institutions and countries and to understand the thematic evolution and to elicit trends through the synthesis of knowledge structures including conceptual, intellectual and social structures of the field.

Findings

Motor themes, basic transverse, niche and emerging and declining themes were identified using (Callon, 1991) strategic thematic map. Besides, four major clusters based on a cocitation network of documents were identified: empirical evidence and drivers of momentum returns, theories explaining momentum returns and implications for asset pricing and market efficiency, avoiding momentum crashes and momentum in alternative asset classes, alternative explanations for momentum returns. The study infers that momentum research is becoming multidisciplinary given the dominance of behavioral theories and economic aspects in explaining the persistence of momentum profits and offers future research directions.

Research limitations/implications

The study deploys bibliometric analysis, appropriate for deriving insights from the vast extant literature. However, a meta-analysis might offer deeper insights into specific dimensions of the research topic. Besides, the study’s findings are based on Scopus indexed articles analyzed using bibilioshiny; the database and software limitations might have affected the findings.

Practical implications

The study is a ready reckoner for scholars who intend to recognize the evolution of momentum investment strategies, current dynamics and future research direction. The study offers practitioners insights into efficiently designing and deploying momentum investment strategies and ways to avoid momentum crashes.

Social implications

The study offers insights into the irrational behavior and systematic errors committed by market participants that helps regulators and policymakers to direct investors’ educational efforts to minimize systematic behavioral errors and related adverse financial consequences.

Originality/value

This comprehensive study on momentum investment strategies evaluates research trends and current dynamics draws a thematic map, knowledge progression in the field and offers future research directions.
Embracing BIM in its totality: a Total BIM case study
Authors: Disney Oliver , Roupé Mattias , Johansson Mikael , Domenico Leto Alessio
Published: September 2022

Purpose

Building information modeling (BIM) is mostly limited to the design phase where two parallel processes exist, i.e. creating 2D-drawings and BIM. Towards the end of the design process, BIM becomes obsolete as focus shifts to producing static 2D-drawings, which leads to a lack of trust in BIM. In Scandinavia, a concept known as Total BIM has emerged, which is a novel “all-in” approach where BIM is the single source of information throughout the project. This paper's purpose is to investigate the overall concept and holistic approach of a Total BIM project to support implementation and strategy work connected to BIM.

Design/methodology/approach

Qualitative data were collected through eight semi-structured interviews with digitalization leaders from the case study project. Findings were analyzed using a holistic framework to BIM implementation.

Findings

The Total BIM concept was contingent on the strong interdependences between commonly found isolated BIM uses. Four main success factors were identified, production-oriented BIM as the main contractual and legally binding construction document, cloud-based model management, user-friendly on-site mobile BIM software and strong leadership.

Originality/value

A unique case is studied where BIM is used throughout all project phases as a single source of information and communication platform. No 2D paper drawings were used on-site and the Total BIM case study highlights the importance of a new digitalized construction process.
The co-evolution of data and the modern enterprise
Authors: Anderson Cindy , Dencik Jacob , Marshall Anthony , Teer Raj
Published: September 2022

Purpose

Central to the corporate response to decades of discontinuity has been a rapidly growing and increasingly impactful role of data and digital technologies. This article studies that co-evolution.

Design/methodology/approach

Reviewing annual and semi-annual IBM survey data from CEO interviews across the past 20 years, IBM Institute for Business Value research has gained a unique perspective on how corporate leaders have used data technology to stay at the vanguard of change.

Findings

In 2021, 62 percent of the highest performing CEOs said technology infrastructure, specifically data infrastructure, is the greatest challenge for their organization.

Practical Implications

From coping with uncertainty, change and complexity, to the need for more actionable insights, to empowering the individualization of the customer experience and engagement with ecosystem partners, data and digital technologies are now central to helping organizations address their top challenges.

Originality Value

Business leaders must think strategically about how the interplay between data, technology and business transformation will need to be shaped to catalyze even more value for the enterprise and its customers.