bluuebunny/crossref_metadata_2025 huggingface dataset:
The Medieval Glass Mosaic of S. Agnese fuori le mura in Rome: Multispectral Imaging for Preliminary Identification of Original Tesserae
Authors: Deiana Rita , Silvestri Alberta , Gianandrea Manuela , Maltoni Sarah , Croci Chiara
Published: March 2023

The mosaic in the apse of the Church of S. Agnese fuori le mura in Rome represents one of the most important examples of the wall mosaics of the Roman Middle Ages. Although it is associated with Byzantine figurative culture, no scientific study has addressed this important piece of Italian art history. One factor that has probably limited and created difficulties for its analysis is the poor legibility of the original parts, which are compromised by heavy restorations affecting the gold background, the faces of the two male side figures, and the lower band of the mosaic. The present work describes how multispectral imaging provided significant guidance in the preliminary identification of possible original areas in this ancient wall mosaic. Through an interdisciplinary approach, the art historical background and historical graphic documentation of known restorations supported the use of multispectral imaging to recognize original parts. The initial results of the lab analyses (SEM-EDS and EMPA) of supposed original tesserae validated the hypothesis made a priori thanks to multispectral acquisition, opening up new application possibilities for use of this noninvasive technique in the preliminary in situ identification of original parts in restored glass wall mosaics.
An Overview of Geophysical Techniques and Their Potential Suitability for Archaeological Studies
Authors: Martorana Raffaele , Capizzi Patrizia , Pisciotta Antonino , Scudero Salvatore , Bottari Carla
Published: March 2023

The need to study, protect, and conserve archaeological heritage has enhanced the application of geophysical techniques as non-invasive and reliable tools to investigate fragile and valuable assets. This review presents the most popular geophysical techniques suitable for archaeogeophysical investigations, namely, magnetometry, ground penetrating radar, and electrical resistivity tomography, together with a series of multiparametric measures taken from aerial platforms (UAS). For each method, we recall the basic physical principles, illustrate the operative procedures for field investigation, and provide indications about data processing and modeling. We propose a flowchart to address reliable and effective geophysical investigations, from its planning to the development of the final archaeogeophysical model. We underline the integrated approach, in which the combination of various techniques allows the best results in terms of resolution, coverage, investigation depth, speed, and costs to be obtained. We introduce a suite of studied cases in which this approach has been applied successfully.
More Than Colonial Vestige? Afternoon Tea as Heritage Practice in Penang (Malaysia)
Authors: Pampus Mareike
Published: March 2023

This article builds on insights from both critical heritage studies and Indian Ocean studies to argue that there is a need to delve deeply into how, by whom, and why heritage is practiced. Empirically, the practice of afternoon tea in Penang (Malaysia) provides insights into habits, manners, and customs in order to explore heritage as a social practice. Drawing on ethnographic field research on the island of Penang in Malaysia, this article demonstrates how the multiple forms, as well as the changing production, practice, and preservation, of local cultural heritage are used to strategically and situationally create identities in dependence of the “other” and in response to a national heritage narrative. In contrast to what is nowadays often considered a colonial vestige, I maintain that these cultural heritage elements are not foreign but part of specific local cultural heritages.
Virus-Induced Silencing of a Sequence Coding for Loricrin-like Protein in Phytophthora infestans upon Infection of a Recombinant Vector Based on Tobacco Mosaic Virus
Authors: Labarile Rossella , Mincuzzi Annamaria , Spanò Roberta , Mascia Tiziana
Published: March 2023

Phytophthora infestans is the oomycete responsible for late blight disease of Solanaceae that causes both yield and economic losses. With the aim of reducing plant wilt and high management costs mainly due to wide fungicide applications, alternative eco-sustainable control strategies are needed. RNA interference (RNAi) is a powerful tool for gene function studies that can be accomplished by constitutive transformation or transient expression such as virus-induced gene silencing (VIGS) experiments. VIGS makes use of viruses to deliver sequences homologous to a target gene fragment and trigger RNAi. Indeed, a P. infestans ortholog of plant loricrin-like protein (LLP), named PiLLP, has been silenced using the direct infection of a recombinant vector based on the plant virus tobacco mosaic virus (TMV-PiLLP-1056), aiming to reduce the oomycete sexual reproduction. For this purpose, the gene coding for the green fluorescent protein (GFP) present in the TMV-GFP-1056 vector has been replaced with an antisense construct obtained by fusion PCR of the PiLLP 5′-UTR and 3′-UTR sequences. Here, we show that RNAi can be expressed in the A1 mating type of P. infestans strain 96.9.5.1 by VIGS using the direct infection of TMV-PiLLP-1056. We provide evidence that the recombinant vector can enter, replicate, and persist in mycelia of P. infestans where it induces the partial downregulation of the PiLLP transcript. Compared with the wild-type, the PiLLP-silenced A1 mating type had slower colony growth and a diminished virulence in detached tomato leaflets. This seems to be the first evidence of a constitutive gene downregulation of P. infestans using a recombinant vector based on a plus-sense RNA plant virus.
The Impact of Meteorological Parameters and Seasonal Changes on Reporting Patients with Selected Cardiovascular Diseases to Hospital Emergency Departments: A Pilot Study
Authors: Kotecki Paweł , Więckowska Barbara , Stawińska-Witoszyńska Barbara
Published: March 2023

(1) Background: So far, research results have confirmed the relationship between heat and cold stress, the fluctuations in atmospheric pressure and high relative humidity, and the vulnerability of patients with so-called “weather-dependent” diseases which could lead to death. This study aimed to determine the meteorological parameters, their interactions, and the seasonal changes of the most significant factors in predicting the number of patients reporting to the Emergency Departments (EDs) in Poznań (Poland) during 2019. (2) Methods: The analysis included the meteorological parameters and data of 3606 patients diagnosed with essential or complicated arterial hypertension, myocardial infarction, chronic ischemic heart disease, and ischemic or unspecified stroke by the International Classification of Diseases (ICD-10). The meteorological data (days per week and seasonal data) were used to build a linear regression model to assess the changes in the daily number of reporting patients. The input data for the final model were selected based on the principal component analysis (PCA), and built for each delay and acceleration (reporting up to 3 days before the change or up to 3 days after the change of the meteorological parameter). (3) Results: A significantly lower number of reports was observed during weekends compared to working days (standardised b = −0.254, p-value < 0.0001) and three days before the maximum daily air temperature in the spring and summer period (standardised b = −0.748, p-value < 0.0001), while two days after the increase in the daily amplitude of atmospheric pressure (standardised b = 0.116, p-value = 0.0267), and also on the day of occurrence of the unfavourable interdiurnal air temperature change, an increase in the number of patients was noted (standardised b = 0.115, p-value = 0.0186). The changes in the last two parameters were statistically insignificant. Based on the obtained results, the negative impact of the changes in the meteorological conditions on the number of reports to the EDs in Poznań was determined.
Influence of Successive Wheelchair Tennis Matches on Handgrip Strength in High-Level Male Players
Authors: Sánchez-Pay Alejandro , Pino-Ortega José , Sanz-Rivas David
Published: March 2023

The purpose of this study was to examine the effects on upper strength in high-level male players playing four successive wheelchair tennis (WT) matches. Eight international WT players took part in a competition, playing one match per day over four consecutive tournament days. Before and after the match, the maximal isometric handgrip strength was measured on the dominant and non-dominant hand. Additionally, each player was equipped with one radiofrequency and IMU device on their wheelchair to control his activity profile (distance). The results showed significant differences between successive matches, with decreasing dominant handgrip strength (p = 0.02, η2 = 0.043), and there was a significant interaction between successive matches and the accumulated distance (p = 0.013, η2 = 0.049). The pre- and post-match strength values of the dominant hand decreased throughout the matches over a number of days, and post hoc analysis showed differences between the first and fourth matches only in pre-match strength (49.06 ± 6.96 vs. 45.94 ± 7.1; p = 0.045; ES: 1.04) but not in the non-dominant hand. Successive matches caused a decrease in the strength values of the WT players, mainly in the dominant hand. These results should be taken into account in the recovery and prevention of injuries in competitions with successive matches.
Perceived Occupational Noise Exposure and Depression in Young Finnish Adults
Authors: Heinonen-Guzejev Marja , Whipp Alyce M. , Wang Zhiyang , Ranjit Anu , Palviainen Teemu , van Kamp Irene , Kaprio Jaakko
Published: March 2023

We investigated the association between perceived occupational noise exposure and depressive symptoms in young Finnish adults and whether noise sensitivity moderates this association. This study was based on an ongoing longitudinal twin study. We included those who had been working daily (n = 521) or weekly (n = 245) during the past 12 months (mean age 22.4, SD 0.7, 53% female). We asked about occupational noise exposure at age 22 and assessed depressive symptoms using the General Behavior Inventory (GBI) at age 17 and 22. Noise sensitivity and covariates were used in linear regression models. Perceived daily occupational noise exposure was associated, as a statistically independent main effect with depressive symptoms at age 22 (beta 1.19; 95% CI 0.09, 2.29) among all, and separately for females (beta 2.22; 95% CI 0.34, 4.09) but not males (beta 0.22; 95% CI −1.08, 1.52). Noise sensitivity was independently associated with depressive symptoms among all (beta 1.35; 95% CI 0.54, 2.17), and separately for males (beta 1.96; 95% CI 0.68, 3.24) but not females (beta 1.05; 95 % CI −0.04, 2.13). Noise sensitivity was independent of perceived occupational noise exposure. Pre-existing depressive symptoms at age 17 were predictive of perceived occupational noise exposure, suggesting complex interactions of noise and depression.
Profiling Particles of Sahara Dust Settled on the Ground by a Simplified Dynamic Light Scattering Procedure and Sedimentation
Authors: Chicea Dan , Olaru Sorin
Published: March 2023

Dust particles exist in the form of mineral aerosols and play a significant role in climate change patterns, while also having the potential to affect human health. The size of these particles is crucial, as it determines the atmosphere’s albedo. In the past few years, a Saharan dust cloud has moved and arrived above Romania during spring, followed by rain containing the dust particles, which are deposited on various objects. We collected these particles in an aqueous suspension and employed natural sedimentation to separate them by density. We then conducted a dynamic light scattering (DLS) experiment to analyze their size. Our DLS setup was straightforward, and the time series analysis involved evaluating the frequency spectrum of the scattered light intensity—also known as the power spectrum—filtering it, and fitting the expected Lorentzian line to it to determine the parameters and the average diameter of the suspended particles. We found that the dust particles had a continuous distribution, with the biggest particles having a diameter around 1100 nm. The results obtained from the combination of sedimentation and DLS are consistent with reports on the size of Saharan dust particles in other regions of Europe.
Marital Status, Father Acknowledgement, and Birth Outcomes: Does the Maternal Education Matter?
Authors: Merklinger-Gruchala Anna , Kapiszewska Maria
Published: March 2023

We evaluated whether the maternal marital status and father acknowledgement (proxy for paternal presence) affect birth weight, and if so, whether the maternal educational attainment modifies this effect. The growing tendency of alternative forms of family structure affects maternal well-being and pregnancy outcome. However, it is not known whether poorer birth outcomes of out-of-wedlock childbearing can be overcome or compensated by maternal education. Using birth registry data, we assessed the impact of maternal civil status and child recognition by the father on birth-weight-for-gestational age (BWGA) z-scores, with respect to maternal education, among Polish mothers (N = 53,528). After standardization, the effect of being unmarried with father acknowledgement (UM-F) vs. married with father acknowledgement (M-F) reduced the BWGA z-score of 0.05 (p < 0.001), irrespective of educational attainment (p for interaction = 0.79). However, education differentiated the effect of father acknowledgement across unmarried mothers. BWGA z-scores were significantly lower among the low-educated unmarried group without father acknowledgment (UM-NF) as compared to UM-F (equaled −0.11, p = 0.01). The same effect among the higher-educated group was non-significant (p = 0.72). Higher maternal education can compensate for the negative effect of a lack of father acknowledgement, but it does not help to overcome the effect of out-of-wedlock stress exposure.
Taking Charge: Social Support Dynamics among Older Adults and Their Significant Others in COVID-19 Vaccination and Mitigation Efforts
Authors: Brennan-Ing Mark , Wu Yiyi , Manalel Jasmine A. , Finkelstein Ruth
Published: March 2023

Older people have been disproportionately affected by the COVID-19 pandemic and are often portrayed as passive victims of this global health crisis. However, older adults do take responsibility for their own health and that of others in large part through social network dynamics. The purpose of this study was to understand the processes whereby older adults’ social networks shape their own health behaviors, and vice versa, in the context of COVID-19 vaccination and other mitigation efforts. Qualitative data from 77 older adults between ages 65 and 94 obtained through focus groups or individual interview participants were analyzed. Participant narratives demonstrated the reciprocal nature of social support and health behaviors and provided evidence that COVID-19-related health behaviors in this population were motivated by social support, altruism, and life experience. These findings emphasize older adults’ active role as health promoters in their families and communities, keeping themselves and their significant others safe from COVID infection. Implications for the role of older adults in community health promotion efforts are discussed.
Aspects of Dental Occlusion Assessed with the T-Scan System among a Group of Romanian Dental Students in a Cross-Sectional Study
Authors: Vlăduțu Diana Elena , Ionescu Mihaela , Noveri Lorenzo , Manolea Horia Octavian , Scrieciu Monica , Popescu Sanda Mihaela , Turcu Adina Andreea , Ştefârță Alexandru , Lăzărescu Grigore , Mercuț Veronica
Published: March 2023

This study evaluated the occlusal relationships in students with bruxism, using the T-Scan III system, and their correlation with the activity of the masticatory muscles assessed through surface electromyography (sEMG). The study group was divided into two subgroups (based on self-reporting): 20 participants with possible bruxism and 20 participants without possible bruxism; all participants underwent the following evaluations: sEMG recordings using the dia-BRUXO device for masticatory muscles assessment, as well as static and dynamic occlusion using the T-SCAN III system. The analysis of the maximum intercuspidal (MI) position revealed a positive moderate association between the values of the occlusal forces in MI distributed along the two hemiarches, and the number of grinding events during daytime, which was statistically significant (p < 0.05). The analysis of protrusion movements reflected statistically significant differences between the non-working interferences and sEMG parameters specific to bruxism (p < 0.05). The analysis of laterotrusion movements indicated that participants with anterolateral guidance presented higher values of awake bruxism indexes and higher values of clenching events during nighttime. For all three mandibular movements, their duration was higher for the study group compared to the control group. Therefore, this study confirmed the utility of sEMG recordings in the bruxism diagnosis process, as well as the association between dental occlusion and bruxism.
Synthesis, Characterization and Application of a MIP-polyHIPE for Selective Extraction of Angiotensin II Receptor Antagonists Residues in Natural Waters
Authors: Speltini Andrea , Alberti Giancarla , Rovida Riccardo , Milanese Chiara , De Soricellis Giulia , Rinaldi Francesca , Massolini Gabriella , Gallo Angelo , Calleri Enrica
Published: March 2023

Polymers via high internal phase emulsion (polyHIPEs) were molecularly imprinted with Irbesartan, an antihypertensive drug belonging to the class of angiotensin II receptor antagonists (sartan drugs), chosen for the proof-of-concept extraction of hazardous emerging contaminants from water. Different analyte-functional monomer molar ratios (1:100, 1:30 and 1:15) were investigated, and the MIP polyHIPEs have been characterized, parallel to the not imprinted polymer (NIP), by batch sorption experiments. The material with the highest template-functional monomer ratio was the best for Irbesartan removal, showing a sorption capacity fivefold higher than the NIP. Regarding the adsorption kinetics, the analyte–sorbent equilibrium was reached after about 3 h, and the film diffusion model best fitted the kinetic profile. Selectivity was further demonstrated by testing Losartan, another sartan drug, observing a fourfold lower sorption capacity, but still higher than that of NIP. The polymers were also synthesized in cartridges for solid-phase extraction (SPE), which was helpful for evaluating the breakthrough curves and performing pre-concentrations. These have been done in tap and river water samples (100–250 mL, 15–500 µg L−1 Irbesartan), obtaining quantitative sorption/desorption on the MIP-polyHIPE (RSD < 14%, n = 3). The NIP provided a recovery of just around 30%, evidence of partial uptake of the target from water.
Heterogeneous Electro-Fenton-Catalyzed Degradation of Rhodamine B by Nano-Calcined Pyrite
Authors: Tan Yu , Zhao Changsheng , Chen Qingfeng , Li Luzhen , Wang Xinghua , Guo Beibei , Zhang Bowei , Wang Xiaokai
Published: March 2023

The use of natural pyrite as a catalyst for the treatment of recalcitrant organic wastewater by an electro-Fenton system (pyrite-EF) has recently received extensive attention. To improve the catalytic activity of natural pyrite (Py), magnetic pyrite (MPy), and pyrrhotite (Pyr), they were obtained by heat treatment, and the nanoparticles were obtained by ball milling. They were characterized by X-ray diffraction, X-ray electron spectroscopy, and scanning electron microscopy. The degradation performance of rhodamine B (Rhb) by heterogeneous catalysts was tested under the pyrite-EF system. The effects of optimal pH, catalyst concentration, and current density on mineralization rate and mineralization current efficiency were explored. The results showed that the heat treatment caused the phase transformation of pyrite and increased the relative content of ferrous ions. The catalytic performance was MPy > Py > Pyr, and the Rhb degradation process conformed to pseudo-first-order kinetics. Under the optimum conditions of 1 g L−1 MPy, an initial pH of five, and a current density of 30 mA cm−2, the degradation rate and TOC removal rate of Rhb wastewater reached 98.25% and 77.06%, respectively. After five cycles of recycling, the chemical activity of MPy was still higher than that of pretreated Py. The main contribution to Rhb degradation in the system was •OH radical, followed by SO4•−, and the possible catalytic mechanism of MPy catalyst in the pyrite-EF system was proposed.
Digitization, Visualization and Accessibility of Globe Virtual Collection: Case Study Jüttner’s Globe
Authors: Štefanová Eva , Novotná Eva , Čábelka Miroslav
Published: March 2023

The aim of the article is to prepare a model for making available metadata and digital objects of the new Globe Virtual Collection for the Map Collection of the Faculty of Science of Charles University. The globes are special cartographic documents; therefore, they are also described in a special way. The article deals with the digitization, visualization and accessibility of an old globe by Josef Jüttner from 1839, which comes from the depository of one of the most important central European collections. A simple model for a new virtual processing of the globe collection at Charles University is presented. SfM-MVS photogrammetry was chosen for digitization of the globe. The basic elements of the copperplate were set as basic parameters for image acquisition. Contrast, density, black line, line, dash and dot patterns and their complex use were observed for a good graphic design of the globe. Other parameters included a closer determination of the users for whom the resulting product is intended, as well as the profile of the users’ behavior on the site so far. New metadata were extracted from the bibliographic description. The virtual 3D globe was integrated into the database using the Cesium JavaScript library. Metadata and a 3D model of the globe were linked together and made available to the general public on the Globe page of the Map Collection of the Faculty of Science of Charles University. A comparison of web browsers was performed focusing on the loading time of the 3D model on the website. New graphic elements were identified with the new processing. It was possible to read the factual information written on the globe. Different possibilities and limitations of metadata description, photogrammetric methods and web presentation are described. This good practice can be applied by other virtual map collections.
THC and CBD: Villain versus Hero? Insights into Adolescent Exposure
Authors: Pintori Nicholas , Caria Francesca , De Luca Maria Antonietta , Miliano Cristina
Published: March 2023

Cannabis is the most used drug of abuse worldwide. It is well established that the most abundant phytocannabinoids in this plant are Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD). These two compounds have remarkably similar chemical structures yet vastly different effects in the brain. By binding to the same receptors, THC is psychoactive, while CBD has anxiolytic and antipsychotic properties. Lately, a variety of hemp-based products, including CBD and THC, have become widely available in the food and health industry, and medical and recreational use of cannabis has been legalized in many states/countries. As a result, people, including youths, are consuming CBD because it is considered “safe”. An extensive literature exists evaluating the harmful effects of THC in both adults and adolescents, but little is known about the long-term effects of CBD exposure, especially in adolescence. The aim of this review is to collect preclinical and clinical evidence about the effects of cannabidiol.
Fer and FerT: A New Regulatory Link between Sperm and Cancer Cells
Authors: Nir Uri , Grinshtain Elina , Breitbart Haim
Published: March 2023

Fer and its sperm and cancer specific variant, FerT, are non-receptor tyrosine kinases which play roles in cancer progression and metastasis. Recent studies have shed light on the regulatory role of these kinases in ensuring proper sperm function. Comparison of the regulatory cascades in which Fer and FerT are engaged in sperm and cancer cells presents an interesting picture, in which similar regulatory interactions of these enzymes are integrated in a similar or different regulatory context in the two cell types. These diverse compositions extend from the involvement of Fer in modulation of actin cytoskeleton integrity and function, to the unique regulatory interactions of Fer with PARP-1 and the PP1 phosphatase. Furthermore, recent findings link the metabolic regulatory roles of Fer and FerT in sperm and cancer cells. In the current review, we discuss the above detailed aspects, which portray Fer and FerT as new regulatory links between sperm and malignant cells. This perspective view can endow us with new analytical and research tools that will deepen our understanding of the regulatory trajectories and networks that govern these two multi-layered systems.
Dimeric Ankyrin with Inverted Module Promotes Bifunctional Property in Capturing Capsid to Impede HIV-1 Replication
Authors: Juntit On-anong , Sornsuwan Kanokporn , Wisitponchai Tanchanok , Sanghiran Lee Vannajan , Sakkhachornphop Supachai , Yasamut Umpa , Tayapiwatana Chatchai
Published: March 2023

Several anti-HIV scaffolds have been proposed as complementary treatments to highly active antiretroviral therapy. AnkGAG1D4, a designed ankyrin repeat protein, formerly demonstrated anti-HIV-1 replication by interfering with HIV-1 Gag polymerization. However, the improvement of the effectiveness was considered. Recently, the dimeric molecules of AnkGAG1D4 were accomplished in enhancing the binding activity against HIV-1 capsid (CAp24). In this study, the interaction of CAp24 against the dimer conformations was elucidated to elaborate the bifunctional property. The accessibility of the ankyrin binding domains was inspected by bio-layer interferometry. By inverting the second module of dimeric ankyrin (AnkGAG1D4NC-CN), the CAp24 interaction KD was significantly reduced. This reflects the capability of AnkGAG1D4NC-CN in simultaneously capturing CAp24. On the contrary, the binding activity of dimeric AnkGAG1D4NC-NC was indistinguishable from the monomeric AnkGAG1D4. The bifunctional property of AnkGAG1D4NC-CN was subsequently confirmed in the secondary reaction with additional p17p24. This data correlates with the MD simulation, which suggested the flexibility of the AnkGAG1D4NC-CN structure. The CAp24 capturing capacity was influenced by the distance of the AnkGAG1D4 binding domains to introduce the avidity mode of AnkGAG1D4NC-CN. Consequently, AnkGAG1D4NC-CN showed superior potency in interfering with HIV-1 NL4-3 WT and HIV-1 NL4-3 MIRCAI201V replication than AnkGAG1D4NC-NC and an affinity improved AnkGAG1D4-S45Y.
BGP-15 Protects against Doxorubicin-Induced Cell Toxicity via Enhanced Mitochondrial Function
Authors: Gyongyosi Alexandra , Csaki Nikolett , Peto Agota , Szoke Kitti , Fenyvesi Ferenc , Bacskay Ildiko , Lekli Istvan
Published: March 2023

Doxorubicin (DOX) is an efficacious and commonly used chemotherapeutic agent. However, its clinical use is limited due to dose-dependent cardiotoxicity. Several mechanisms have been proposed to play a role in DOX-induced cardiotoxicity, such as free radical generation, oxidative stress, mitochondrial dysfunction, altered apoptosis, and autophagy dysregulation. BGP-15 has a wide range of cytoprotective effects, including mitochondrial protection, but up to now, there is no information about any of its beneficial effects on DOX-induced cardiotoxicity. In this study, we investigated whether the protective effects of BGP-15 pretreatment are predominantly via preserving mitochondrial function, reducing mitochondrial ROS production, and if it has an influence on autophagy processes. H9c2 cardiomyocytes were pretreated with 50 μM of BGP-15 prior to different concentrations (0.1; 1; 3 μM) of DOX exposure. We found that BGP-15 pretreatment significantly improved the cell viability after 12 and 24 h DOX exposure. BGP-15 ameliorated lactate dehydrogenase (LDH) release and cell apoptosis induced by DOX. Additionally, BGP-15 pretreatment attenuated the level of mitochondrial oxidative stress and the loss of mitochondrial membrane potential. Moreover, BGP-15 further slightly modulated the autophagic flux, which was measurably decreased by DOX treatment. Hence, our findings clearly revealed that BGP-15 might be a promising agent for alleviating the cardiotoxicity of DOX. This critical mechanism appears to be given by the protective effect of BGP-15 on mitochondria.
Kinase Inhibitors in Genetic Diseases
Authors: D’Antona Lucia , Amato Rosario , Brescia Carolina , Rocca Valentina , Colao Emma , Iuliano Rodolfo , Blazer-Yost Bonnie L. , Perrotti Nicola
Published: March 2023

Over the years, several studies have shown that kinase-regulated signaling pathways are involved in the development of rare genetic diseases. The study of the mechanisms underlying the onset of these diseases has opened a possible way for the development of targeted therapies using particular kinase inhibitors. Some of these are currently used to treat other diseases, such as cancer. This review aims to describe the possibilities of using kinase inhibitors in genetic pathologies such as tuberous sclerosis, RASopathies, and ciliopathies, describing the various pathways involved and the possible targets already identified or currently under study.
Development of an Anti-Idiotype Aptamer-Based Electrochemical Sensor for a Humanized Therapeutic Antibody Monitoring
Authors: Nagata Madoka , Lee Jinhee , Saito Taro , Ikebukuro Kazunori , Sode Koji
Published: March 2023

Therapeutic monoclonal antibodies (mAbs) are currently the most effective medicines for a wide range of diseases. Therefore, it is expected that easy and rapid measurement of mAbs will be required to improve their efficacy. Here, we report an anti-idiotype aptamer-based electrochemical sensor for a humanized therapeutic antibody, bevacizumab, based on square wave voltammetry (SWV). With this measurement procedure, we were able to monitor the target mAb within 30 min by employing the anti-idiotype bivalent aptamer modified with a redox probe. A fabricated bevacizumab sensor achieved detection of bevacizumab from 1–100 nM while eliminating the need for free redox probes in the solution. The feasibility of monitoring biological samples was also demonstrated by detecting bevacizumab in the diluted artificial serum, and the fabricated sensor succeeded in detecting the target covering the physiologically relevant concentration range of bevacizumab. Our sensor contributes to ongoing efforts towards therapeutic mAbs monitoring by investigating their pharmacokinetics and improving their treatment efficacy.
Effects of Osmotic Stress on the mRNA Expression of prl, prlr, gr, gh, and ghr in the Pituitary and Osmoregulatory Organs of Black Porgy, Acanthopagrus schlegelii
Authors: Nagarajan Ganesan , Aruna Adimoolam , Chang Yu-Ming , Alkhamis Yousef Ahmed , Mathew Roshmon Thomas , Chang Ching-Fong
Published: March 2023

In euryhaline teleost black porgy, Acanthopagrus schlegelii, the glucocorticoid receptor (gr), growth hormone receptor (ghr), prolactin (prl)-receptor (prlr), and sodium–potassium ATPase alpha subunit (α-nka) play essential physiological roles in the osmoregulatory organs, including the gill, kidney, and intestine, during osmotic stress. The present study aimed to investigate the impact of pituitary hormones and hormone receptors in the osmoregulatory organs during the transfer from freshwater (FW) to 4 ppt and seawater (SW) and vice versa in black porgy. Quantitative real-time PCR (Q-PCR) was carried out to analyze the transcript levels during salinity and osmoregulatory stress. Increased salinity resulted in decreased transcripts of prl in the pituitary, α-nka and prlr in the gill, and α-nka and prlr in the kidney. Increased salinity caused the increased transcripts of gr in the gill and α-nka in the intestine. Decreased salinity resulted in increased pituitary prl, and increases in α-nka and prlr in the gill, and α-nka, prlr, and ghr in the kidney. Taken together, the present results highlight the involvement of prl, prlr, gh, and ghr in the osmoregulation and osmotic stress in the osmoregulatory organs (gill, intestine, and kidney). Pituitary prl, and gill and intestine prlr are consistently downregulated during the increased salinity stress and vice versa. It is suggested that prl plays a more significant role in osmoregulation than gh in the euryhaline black porgy. Furthermore, the present results highlighted that the gill gr transcript’s role was solely to balance the homeostasis in the black porgy during salinity stress.
Leading Edge Bumps for Flow Control in Air-Cooled Condensers
Authors: Tieghi Lorenzo , Delibra Giovanni , van der Spuy Johan , Corsini Alessandro
Published: March 2023

Air-cooled condensers (ACCs) are commonly found in power plants working with concentrated solar power or in steam power plants operated in regions with limited water availability. In ACCs, the flow of air is driven toward the heat exchangers by axial fans that are characterized by large diameters and operate at very high mass flow rates with a near-zero static pressure rise. Given the overall requirements in steam plants, these fans are subjected to inflow distortions, unstable operations, and are characterized by high noise emissions. Previous studies show that leading edge bumps in the tip region of axial fans can effectively reduce the sound pressure levels without affecting the static efficiency. Nevertheless, the effects of this treatment in terms of flow patterns and heat exchange in the whole ACC system were not investigated. In this work, the effect of leading edge bumps on the flow patterns is analyzed. Two RANS simulations were carried out using OpenFOAM on a simplified model of the air-cooled condenser. The fans are simulated using a frozen rotor approach. Turbulence modeling relies on the RNG k-epsilon model. The fan is characterized by a diameter of 7.3 m and a 333 m3/s volumetric flow rate at the design point. The presence of the heat exchanger is modeled using a porous medium. The comparison between the flow fields clearly exerts that the modified blade is responsible for the redistribution of radial velocities in the rotor region. This drastically reduces the losses related to the installation of the fan in a real configuration.
Transient 3D CFD Simulation of a Pelton Turbine—A State-of-the-Art Approach for Pelton Development and Optimisation
Authors: Sandmaier Lukas , Meusburger Peter , Benigni Helmut
Published: March 2023

The complex flow conditions in Pelton turbines make it challenging to gain detailed insight into the local flow processes. However, CFD methods offer vast potential for developing and optimising Pelton turbines due to these flow conditions. In a comprehensive examination, a six-nozzle prototype Pelton turbine with 19 buckets has been investigated using 3D CFD simulations. First, the steady simulations of the manifold and the unsteady runner simulation have been performed with a mesh-based, commercial CFD code, whereby a two-equation turbulence model and the homogeneous two-phase model were used. Then, to limit the simulation time, symmetry was applied in the runner simulation, and also a strategic definition of the mesh element size in selected blocks of higher interest. Subsequently, the simulation results were analysed. Based on the first simulation results, the geometry of the distributor was modified in an iterative process to reduce losses and improve the jet shape. For the improvement of the latter, a characteristic number was introduced to quantify the secondary flows upstream of the nozzles, which act negatively on the jet shape. Furthermore, the results of the runner simulation were analysed with special regard to the jet-bucket interaction from the start to the end of the impingement cycle of a particular bucket. Finally, a potential efficiency increase could be derived from the summary.
Ontology Learning Applications of Knowledge Base Construction for Microelectronic Systems Information
Authors: Wawrzik Frank , Rafique Khushnood Adil , Rahman Farin , Grimm Christoph
Published: March 2023

Knowledge base construction (KBC) using AI has been one of the key goals of this highly popular technology since its emergence, as it helps to comprehend everything, including relations, around us. The construction of knowledge bases can summarize a piece of text in a machine-processable and understandable way. This can prove to be valuable and assistive to knowledge engineers. In this paper, we present the application of natural language processing in the construction of knowledge bases. We demonstrate how a trained bidirectional long short-term memory or bi-LSTM neural network model can be used to construct knowledge bases in accordance with the exact ISO26262 definitions as defined in the GENIAL! Basic Ontology. We provide the system with an electronic text document from the microelectronics domain and the system attempts to create a knowledge base from the available information in textual format. This information is then expressed in the form of graphs when queried by the user. This method of information retrieval presents the user with a much more technical and comprehensive understanding of an expert piece of text. This is achieved by applying the process of named entity recognition (NER) for knowledge extraction. This paper provides a result report of the current status of our knowledge construction process and knowledge base content, as well as describes our challenges and experiences.
Testing Road Vehicle User Interfaces Concerning the Driver’s Cognitive Load
Authors: Nagy Viktor , Kovács Gábor , Földesi Péter , Kurhan Dmytro , Sysyn Mykola , Szalai Szabolcs , Fischer Szabolcs
Published: March 2023

This paper investigates the usability of touch screens used in mass production road vehicles. Our goal is to provide a detailed comparison of conventional physical buttons and capacitive touch screens taking the human factor into account. The pilot test focuses on a specific Non-driving Related Task (NDRT): the control of the on-board climate system using a touch screen panel versus rotating knobs and push buttons. Psychological parameters, functionality, usability and, the ergonomics of In-Vehicle Information Systems (IVIS) were evaluated using a specific questionnaire, a system usability scale (SUS), workload assessment (NASA-TLX), and a physiological sensor system. The measurements are based on a wearable eye-tracker that provides fixation points of the driver’s gaze in order to detect distraction. The closed road used for the naturalistic driving study was provided by the ZalaZONE Test Track, Zalaegerszeg, Hungary. Objective and subjective results of the pilot study indicate that the control of touch screen panels causes higher visual, manual, and cognitive distraction than the use of physical buttons. The statistical analysis demonstrated that conventional techniques need to be complemented in order to better represent human behavior differences.
On a Benchmark Problem for Modeling and Simulation of Concrete Dams Cracking Response
Authors: Ribeiro Paulo Marcelo Vieira , Léger Pierre
Published: March 2023

Concrete dams are massive unreinforced quasi-brittle structures prone to cracking from multiple causes. The structural safety assessment of cracked concrete dams is typically performed using computational analysis through numerical methods, with adequate representation of the material model. Advances in the last decades including computational processing power, novel material, and numerical models have enabled remarkable progress in the analysis of concrete dams. Nevertheless, classical benchmarks remain reliable references for the performance analysis of these structures. This paper presents the main aspects of modeling and simulation of a concrete gravity dam cracking response based on a broad literature survey. Emphasis is given to an in-depth review of the benchmark problem analyzed by Carpinteri et al. (1992). We then use the Abaqus concrete damage plasticity constitutive model to solve the benchmark problem and provide recommendations to obtain accurate results with an optimal computational cost. The best practices of modeling, simulation, verification, and validation are presented.
Dehydration Alters Transcript Levels in the Mosquito Midgut, Likely Facilitating Rapid Rehydration following a Bloodmeal
Authors: Holmes Christopher J. , Brown Elliott S. , Sharma Dhriti , Warden Matthew , Pathak Atit , Payton Blaine , Nguyen Quynh , Spangler Austin , Sivakumar Jaishna , Hendershot Jacob M. , Benoit Joshua B.
Published: March 2023

The mosquito midgut is an important site for bloodmeal regulation while also acting as a primary site for pathogen exposure within the mosquito. Recent studies show that exposure to dehydrating conditions alters mosquito bloodfeeding behaviors as well as post-feeding regulation, likely altering how pathogens interact with the mosquito. Unfortunately, few studies have explored the underlying dynamics between dehydration and bloodmeal utilization, and the overall impact on disease transmission dynamics remains veiled. In this study, we find that dehydration-based feeding in the yellow fever mosquito, Aedes aegypti, prompts alterations to midgut gene expression, as well as subsequent physiological factors involving water control and post-bloodfeeding (pbf) regulation. Altered expression of ion transporter genes and aquaporin 2 (AQP2) in the midgut of dehydrated mosquitoes as well as the rapid reequilibration of hemolymph osmolality after a bloodmeal indicate an ability to expedite fluid and ion processing. These alterations ultimately indicate that female A. aegypti employ mechanisms to ameliorate the detriments of dehydration by imbibing a bloodmeal, providing an effective avenue for rehydration. Continued research into bloodmeal utilization and the resulting effects on arthropod-borne transmission dynamics becomes increasingly important as drought prevalence is increased by climate change.
Properties of Plywood Bonded with Formaldehyde-Free Adhesive Based on Poly(vinyl alcohol)–Tannin–Hexamine at Different Formulations and Cold-Pressing Times
Authors: Sari Ratih Afrida Lismana , Lubis Muhammad Adly Rahandi , Sari Rita Kartika , Kristak Lubos , Iswanto Apri Heri , Mardawati Efri , Fatriasari Widya , Lee Seng Hua , Reh Roman , Sedliacik Jan , Maulana Muhammad Iqbal , Suryanegara Lisman , Subiyanto Bambang , Maulana Sena
Published: March 2023

The plywood industry’s sustainability, performance, and production costs depend on wood adhesives and the hot pressing technique. In this investigation, a cold-setting plywood adhesive based on polyvinyl alcohol (P), tannin (T), and hexamine (H) was produced. The physical and mechanical properties of plywood were examined at different formulations such as tannin concentration (10% and 20%), hexamine content (5%, 10%, and 15%), and cold-pressing time (3, 6, 12, and 24 h). This study showed that high tannin and hexamine content also increased the solids content, but decreased the average viscosity of the adhesive. Markedly, the cohesion strength of PTH-based adhesives increased from 5.57 Pa at 1/s to 1411.6 Pa at 400/s shear rate, regardless of the adhesive formulation. The shear modulus subsequently decreased as a function of the shear rate and increased with a higher tannin and hexamine content. This study revealed that the higher tannin and hexamine content and longer cold-pressing times could produce plywood with the tested adhesive that met the Japanese standard strength requirements. A combination of PTH-based adhesive prepared with formula 2 and 24 h cold-pressing resulted in the highest TSS value of 1.42 MPa, MOR values of 88.7 MPa, MOE values of 14,025.6 MPa, and wood failure of 47.2%. This study showed the possibility of fabricating eco-friendly plywood panels bonded with PTH-based adhesive using the cold-pressing process as an alternative to conventional plywood.
Why Do Nanoparticles (CNTs) Reduce the Glass Transition Temperature of Nanocomposites?
Authors: Marom Gad
Published: March 2023

This ‘opinion’ article has been undertaken to provide a plausible answer to the question of why nanocomposites that are reinforced by acicular nanoparticles such as carbon nanotubes (CNTs) do not exhibit the anticipated physical properties—particularly, why the glass transition temperature in some compositions exhibits huge decreases, contrary to expectations. It is claimed that this behavior is typical of fully exfoliated, uniformly dispersed nanocomposites, whose structure is that of molecular composites or solid solutions, and which abide by colligative rules.
Thermal Intra-Layer Interaction of Discretized Fractal Exposure Strategies in Non-Isothermal Powder Bed Fusion of Polypropylene
Authors: Schlicht Samuel , Drummer Dietmar
Published: March 2023

Additive manufacturing of material systems sensitive to heat degradation represents an essential prerequisite for the integration of novel functionalized material systems in medical applications, such as the hybrid processing of high-performance thermoplastics and gelling polymers. For enabling an inherent process stability under non-isothermal conditions at reduced ambient temperatures in laser-based additive manufacturing, maintaining a homogeneous layer formation is of vital significance. To minimize crystallization-induced deflections of formed layers while avoiding support structures, the temporal and spatial discretization of the melting process is combined with the subsequent quenching of the polymer melt due to thermal conduction. Based on implementing superposed, phase-shifted fractal curves as the underlying exposure structure, the locally limited temporal and spatial discretization of the exposure process promotes a mesoscale compensation of crystallization shrinkage and thermal distortion, enabling the essential homogeneous layer formation. For improving the understanding of local parameter-dependent thermal intra-layer interactions under non-isothermal processing conditions, geometric boundary conditions of distinct exposure vectors and the underlying laser power are varied. Applying polypropylene as a model material, a significant influence of the spatial distance of fractal exposure structures on the thermal superposition of distinct exposure vectors can be derived, implicitly influencing temporal and temperature-dependent characteristics of the material crystallization and the emerging thermal material exposure. Furthermore, the formation of sub-focus structures can be observed, contributing to the spatial discretization of the layer formation, representing a decisive factor that influences the structure formation and mesoscopic part properties in non-isothermal powder bed fusion of polymers. Consequently, the presented approach represents a foundation for the support-free, accelerated non-isothermal additive manufacturing of both polymers and metals, demonstrating a novel methodology for the mesoscale compensation of thermal shrinkage.