bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of A Concentrate Rich in Agro-Industrial By-Products on Productivity Results, Carcass Characteristics and Meat Quality Traits of Finishing Heifers
Authors: Moreno Díaz María , Domenech García Valeriano , Avilés Ramírez Carmen , Peña Blanco Francisco , Requena Domenech Francisco , Martínez Marín Andrés
Published: July 2020

Finishing diets in intensive beef production systems are mainly based on cereals, which does not take advantage of the capacity of the ruminant digestive system to digest fibrous feeds, cannot be considered sustainable and does not contribute to the circular bioeconomy. Our aim was to investigate the effects of an alternative concentrate rich in agro-industrial by-products for finishing crossbred Limousine heifers. Four pens with 12 heifers and four pens with 13 heifers were randomly allocated to one of two treatments: control (CON), a commercial concentrate with a 43.3% cereal composition, and alternative (ALT), a concentrate with a composition of 22% cereals and 73.5% agro-industrial by-products. Growth performance data were collected along the 91 days of the experimental period. Carcass characteristics were collected after slaughter and 24 h later. Vacuum-packaged samples from longissimus muscle were aged for 7, 21 or 28 days to study meat quality traits. Feed intake was higher and feed conversion rate was poorer in the ALT treatment, but no differences were found in average daily gain and feeding costs. Treatment had no effects on any of the measured carcass traits (grading, hot and cold carcass weight, dressing out, chilling losses, subcutaneous fat depth, pH, temperature and lean and fat colour) nor on the meat quality traits (drip loss, cooking loss, shear force, oxidative stability, chromatic indices and pigment contents). Ageing time decreased drip loss and shear force, increased lightness and did not affect redness or surface colour stability. In conclusion, feeding crossbred Limousine heifers a finishing diet rich in agro-industrial by-products did not have any negative effects on performance, carcass and meat quality traits, which might be considered positive from the point of view of sustainability of beef production. Under the conditions assayed, ageing for 21 and 28 days improved tenderness of meat, without detrimental effects on oxidative stability or traits related to visual acceptability.
Consumer Acceptance of Meat from Animals Reared on Insect Meal as Feed
Authors: Szendrő Katalin , Nagy Mónika Zita , Tóth Katalin
Published: July 2020

Insect protein production requires much less land, feed, and water, and thus has a much smaller ecological footprint than animal protein production, which is important for reducing global warming. Poultry, pigs, and fish consume insects in nature, so insect meal could be a good substitute for soybean and fishmeal as a protein source in diets. The aim of this study was to examine consumer opinion on meat that originated from animals whose diet contained insect meal. The study was conducted in Hungary in 2020 (N = 414). On a scale of 1–7, respondents gave much lower scores (3.96) to this product than that which originated from a free-range system (5.11; p < 0.001). Male, more highly educated, and 30–39 year-old respondents gave significantly higher scores than other groups. The most important factor in accepting insect meal in animal feed was “no risk”, while the least important factor was “replacement of Genetically Modified (GM) soybeans”. Since free-range animals also consume insects, the difference in the attitude of respondents was based on their aversion to insects, while accepting free-range as the best animal welfare system. Thus, more emphasis should be placed on the benefits of insect meal in animal feed in order to bring about awareness and acceptance.
Effects of L-Arginine Supplementation during Late Gestation on Reproductive Performance, Piglet Uniformity, Blood Profiles, and Milk Composition in High Prolific Sows
Authors: Hong Jinsu , Fang Lin Hu , Jeong Jae Hark , Kim Yoo Yong
Published: July 2020

This study was conducted to evaluate the effects of L-arginine supplementation levels during late gestation on reproductive performance and piglet uniformity in high prolific sows. A total of 60 F1 multiparous sows (Yorkshire × Landrace), with an average body weight of 238.2 kg, were allotted to one of three treatment groups in a completely randomized design. The dietary treatments were divided by the supplementation level of arginine during the late-gestation period, from day 70 to farrowing, as follows—(1) CON: corn-soybean meal-based basal diet (Arg 0.72%), (2) Arg10: basal diet + L-Arg 0.28% (Arg 1.0%), and (3) Arg15: basal diet + L-Arg 0.79% (Arg 1.5%). The same lactation diet was provided ad libitum to sows during the lactation period. There were no significant differences in body weight and backfat thickness in sows during late-gestation and lactation. Dietary arginine levels had no significant influences on the number of total born, stillbirth, and born alive. However, increasing inclusion level of L-arginine supplementation tended to increase (p < 0.10) alive litter weight linearly, and also linearly increased (p < 0.05) the piglet weight gain and litter weight gain during the lactation period. In piglet uniformity, the standard deviation of piglet birth weight (p < 0.05) and the coefficient of variation for piglet birth weight (p < 0.10) increased linearly, as dietary arginine levels increased in the late gestation period. Increasing L-arginine supplementation to late gestating sows linearly increased (p < 0.05) the blood concentrations of arginine and ornithine at day 90 and day 110 of gestation. On the other hand, dietary arginine levels in late gestation did not affect the blood parameters related to the nitrogen utilization. Increasing dietary arginine levels for the late gestating sows did not affect the milk composition for colostrum and milk at day 21 of lactation. In conclusion, the inclusion level of arginine in the diet for late gestating sows, by up to 1.5%, could improve the alive litter weight at birth and litter weight gain during lactation, whereas the piglet uniformity at birth was decreased due to the increase of survival for fetuses with light birth weight.
Attention Bias Test Measures Negative But Not Positive Affect in Sheep: A Replication Study
Authors: Monk Jessica E. , Lee Caroline , Dickson Emily , Campbell Dana L. M.
Published: July 2020

An attention bias test has been developed as a measure of negative affective states in sheep. The test measures an individual’s allocation of attention between a threatening (previous location of a dog) and positive (conspecific photo) stimulus over a 3 min period. This study replicated a previously inconclusive study, to determine whether the test could assess positive affective states under more controlled conditions and with a younger population of animals. Pharmacological treatments were used to induce anxious, calm, happy, and control affective states prior to entering the attention bias test arena (n = 20/treatment). We hypothesized that sheep in positive and negative affective states could be differentiated using key measures of attention during testing, including vigilance (head at or above shoulder height) and duration looking towards the valenced stimuli. Anxious sheep were more vigilant than control animals during attention bias testing as predicted (linear mixed effects model, p = 0.002), but the positive groups did not differ from controls (p > 0.05). There was no effect of treatment on looking behaviors (p > 0.05). We suggest this attention bias test paradigm can assess negative but not positive affect in sheep and that modifications to the ethogram or stimuli are needed to more clearly characterize the direction of attention during testing.
Carcass Characteristics, Physicochemical Properties, and Texture and Microstructure of the Meat and Internal Organs of Carrier and King Pigeons
Authors: Kokoszyński Dariusz , Stęczny Kamil , Żochowska-Kujawska Joanna , Sobczak Małgorzata , Kotowicz Marek , Saleh Mohamed , Fik Martin , Arpášová Henrieta , Hrnčár Cyril , Włodarczyk Karol
Published: July 2020

Pigeons have been the subject of research in the past, but the knowledge gained is incomplete and must be extended. The aim of the study was to provide information about differences in carcass weight and measurements, carcass composition, proximate chemical composition, acidity, electrical conductivity, color attributes, the texture, rheological properties and microstructure of the meat, and some biometric characteristics of the digestive system in carrier and King pigeons, and also to determine if the two compared breeds meet the expectations of pigeon meat consumers to the same extent. The study involved 40 carcasses from carrier pigeons and King pigeons after three reproductive seasons. The chemical composition was determined by near-infrared transmission (NIT) spectroscopy, color coordinates according to CIELab, the texture according to Texture Profile Analysis (TPA) and Warner–Bratzler (WB) tests, and the rheological properties of meat according to the relaxation test. The compared pigeon groups differed significantly (p < 0.05) in carcass weight and measurements, carcass composition (except breast muscle percentage), chemical composition (except leg muscle collagen content) and electrical conductivity, lightness (L*), yellowness (b*), chroma (C*) and hue angle (h*), textural characteristics (except cohesiveness and Warner‒Bratzler shear force), rheological properties, microstructure of the pectoralis major muscle, as well as the total length of intestine and its segments, duodenal diameter, weight of proventriculus, gizzard, liver, heart, and spleen. The sex of the birds had a significant (p < 0.05) effect on the carcass weight, chest circumference, carcass neck percentage, breast muscle collagen content, and caeca length. The genotype by sex interaction was significant (p < 0.05) for fat content, collagen content, hardness, sum of elastic moduli and sum of viscous moduli of the pectoralis major muscle, protein and collagen content of leg muscles, duodenal and caecal length, jejunal and ileal diameter, and spleen weight. The obtained results show a significant effect of genetic origin and sex on the nutritive and technological value of the meat, and on the digestive system development of the pigeons.
Avian Satellite Cell Plasticity
Authors: Jankowski Maurycy , Mozdziak Paul , Petitte James , Kulus Magdalena , Kempisty Bartosz
Published: July 2020

Adult myogenesis is dependent on a population of precursor cells, located between the sarcolemma and the basal lamina of the muscle fiber. These satellite cells, usually present in a quiescent state, become activated in response to mechanical muscle strain, differentiating and fusing to add new nuclei to enlarging muscles. As their myogenic lineage commitment is induced on demand, muscle satellite cells exhibit a certain amount of plasticity, possibly being able to be directed to differentiate into non-myogenic fates. In this study, myosatellite cells were isolated from chicken muscle samples, characterized in vitro and introduced into developing blastoderms. They were further investigated using fluorescence microscopy, immunohistochemistry and PCR, to determine their location in embryos after three and eighteen days. The results of the in vitro analysis confirmed that the cells obtained from the Pectoralis thoracicus are highly myogenic, based on the expression of Pax7, Myogenin, MyoD, Desmin and the myotube assay. Furthermore, the investigation of satellite cells within the embryo showed their migration to the regions of Pectoralis thoracicus, heart, liver, gizzard, proventriculus, intestine and brain. Overall, the results of the study proved the high myogenicity of chicken Pectoralis thoracicus cell isolates, as well as provided new information about their migration pathways following introduction into the blastocyst. The presence of the introduced LacZ or eGFP transgenes across the embryo, even 20 days after myosatellite cell injection, further supports the notion that satellite cells exhibit significant plasticity, potentially transdifferentiating into non-muscle lineages.
Bacteriostatic and Bactericidal Effect of Tigecycline on Leptospira spp.
Authors: Bertelloni Fabrizio , Cilia Giovanni , Fratini Filippo
Published: July 2020

Tigecycline is a relatively new antimicrobial, belonging to glycylcyclines with antimicrobial activity against a large spectrum of bacteria. Very few data are available on its effect on Leptospira spp., which consist in a bacteriostatic mechanism. The aim of this investigation was to evaluate the bacteriostatic and bactericidal effect of tigecycline on reference Leptospira strains belonging to 16 serovars. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined through the microdilutions method, and tetracycline was used as the control. Results showed that tigecycline had higher MIC and MBC values than tetracycline. Obtained MIC values were between 4 and 32 µg/mL, while MBC values between 16 and >128 µg/mL. Patoc (MIC: 4 µg/mL; MBC: 16 µg/mL) resulted in the most susceptible serovar, while the most resistant were Bataviae (MIC: 32 µg/mL; MBC: 64 µg/mL), Bratislava (MIC: 8 µg/mL; MBC 128 µg/mL), and Tarassovi (MIC: 8 µg/mL; MBC: >128 µg/mL). This is the first investigation focused on the effect of tigecycline against Leptospira spp. reference strains. Since tigecycline is used as a treatment for bacteremia and urinary tract disease, and these symptoms could be linked to Leptospira infection, the possibility of using this antibiotic as a treatment for leptospirosis should be evaluated. Further studies are needed to explore the possibility to use tigecycline for in vivo application against Leptospira.
Error Detection for Arabic Text Using Neural Sequence Labeling
Authors: Madi Nora , Al-Khalifa Hend
Published: July 2020

The English language has, thus far, received the most attention in research concerning automatic grammar error correction and detection. However, these tasks have been less investigated for other languages. In this paper, we present the first experiments using neural network models for the task of error detection for Modern Standard Arabic (MSA) text. We investigate several neural network architectures and report the evaluation results acquired by applying cross-validation on the data. All experiments involve a corpus we created and augmented. The corpus has 494 sentences and 620 sentences after augmentation. Our models achieved a maximum precision of 78.09%, recall of 83.95%, and F0.5 score of 79.62% in the error detection task using SimpleRNN. Using an LSTM, we achieved a maximum precision of 79.21%, recall of 93.8%, and F0.5 score of 79.16%. Finally, the best results were achieved using a BiLSTM with a maximum precision of 80.74%, recall of 85.73%, and F0.5 score of 81.55%. We compared the results of the three models to a baseline, which is a commercially available Arabic grammar checker (Microsoft Word 2007). LSTM, BiLSTM, and SimpleRNN all outperformed the baseline in precision and F0.5. Our work shows preliminary results, demonstrating that neural network architectures for error detection through sequence labeling can successfully be applied to Arabic text.
Analysis of the Actual Power and EMF Exposure from Base Stations in a Commercial 5G Network
Authors: Colombi Davide , Joshi Paramananda , Xu Bo , Ghasemifard Fatemeh , Narasaraju Vignesh , Törnevik Christer
Published: July 2020

In this work, monitoring of the transmit power for several base stations operating in a live 5G network (Telstra, Australia) was conducted with the purpose of analyzing the radio frequency (RF) electromagnetic field (EMF) exposure levels. The base stations made use of state-of-the-art massive MIMO antennas utilizing beamforming in order to optimize the signal strength at the user’s device. In order to characterize the actual EMF exposure from 5G base stations, knowledge of the amount of power dynamically allocated to each beam is therefore of importance. Experimental data on the spatial distribution of the base stations’ transmit power were gathered directly from the network by extracting information on the radio and baseband operations. Out of more than 13 million samples collected over 24 h, the maximum time-averaged power per beam direction was found to be well-below the theoretical maximum and lower than what was predicted by the existing statistical models. The results show that assuming constant peak power transmission in a fixed beam direction leads to an unrealistic EMF exposure assessment. This work provides insights relevant for the standardization of EMF compliance assessment methodologies applicable for 5G base stations.
On the Flow over High-rise Building for Wind Energy Harvesting: An Experimental Investigation of Wind Speed and Surface Pressure
Authors: Hemida Hassan , Šarkić Glumac Anina , Vita Giulio , Kostadinović Vranešević Kristina , Höffer Rüdiger
Published: July 2020

The human migration from rural to urban areas has triggered a chain reaction causing the spiking energy demand of cities worldwide. High-rise buildings filling the urban skyline could potentially provide a means to improve the penetration of renewable wind energy by installing wind turbines at their rooftop. However, the above roof flow region has not received much attention and most results deal with low-rise buildings. This study investigates the flow pattern above the roof of a high-rise building by analysing velocity and pressure measurements performed in an atmospheric boundary layer wind tunnel, including four wind directions and two different roof shapes. Comparison of the surface pressure patterns on the flat roof with available low-rise building studies shows that the surface pressure contours are consistent for a given wind direction. At 0° wind direction, a separation bubble is detected, while cone vortices dominate at 30° and 45°. The determining factor for the installation of small wind turbines is the vicinity to the roof. Thus, 45° wind direction shows to be the most desirable angle by bringing the substantial amplification of wind and keeping the turbulence intensity low. Decking the roof creates favourable characteristics by overcoming the sensitivity to the wind direction while preserving the speed-up effect.
Residual Echo Suppression Considering Harmonic Distortion and Temporal Correlation
Authors: Song Hyungchan , Shin Jong Won
Published: July 2020

In acoustic echo cancellation, a certain level of residual echo resides in the output of the linear echo canceller because of the nonlinearity of the power amplifier, loudspeaker, and acoustic transfer function in addition to the estimation error of the linear echo canceller. The residual echo in the current frame is correlated not only to the linear echo estimates for the harmonically-related frequency bins in the current frame, but also with linear echo estimates, residual echo estimates, and microphone signals in adjacent frames. In this paper, we propose a residual echo suppression scheme considering harmonic distortion and temporal correlation in the short-time Fourier transform domain. To exploit residual echo estimates and microphone signals in past frames without the adverse effect of the near-end speech and noise, we adopt a double-talk detector which is tuned to have a low false rejection rate of double-talks. Experimental results show that the proposed method outperformed the conventional approach in terms of the echo return loss enhancement during single-talk periods and the perceptual evaluation of speech quality scores during double-talk periods.
Joint Learning of Temporal Models to Handle Imbalanced Data for Human Activity Recognition
Authors: Hamad Rebeen Ali , Yang Longzhi , Woo Wai Lok , Wei Bo
Published: July 2020

Human activity recognition has become essential to a wide range of applications, such as smart home monitoring, health-care, surveillance. However, it is challenging to deliver a sufficiently robust human activity recognition system from raw sensor data with noise in a smart environment setting. Moreover, imbalanced human activity datasets with less frequent activities create extra challenges for accurate activity recognition. Deep learning algorithms have achieved promising results on balanced datasets, but their performance on imbalanced datasets without explicit algorithm design cannot be promised. Therefore, we aim to realise an activity recognition system using multi-modal sensors to address the issue of class imbalance in deep learning and improve recognition accuracy. This paper proposes a joint diverse temporal learning framework using Long Short Term Memory and one-dimensional Convolutional Neural Network models to improve human activity recognition, especially for less represented activities. We extensively evaluate the proposed method for Activities of Daily Living recognition using binary sensors dataset. A comparative study on five smart home datasets demonstrate that our proposed approach outperforms the existing individual temporal models and their hybridization. Furthermore, this is particularly the case for minority classes in addition to reasonable improvement on the majority classes of human activities.
A Novel Artificial Pancreas: Energy Efficient Valveless Piezoelectric Actuated Closed-Loop Insulin Pump for T1DM
Authors: Kaçar Alp , Özer Mehmet Bülent , Taşcıoğlu Yiğit
Published: July 2020

The objective of this work is to develop a closed-loop controlled insulin pump to keep the blood glucose level of Type 1 diabetes mellitus (T1DM) patients in the desired range. In contrast to the existing artificial pancreas systems with syringe pumps, an energy-efficient, valveless piezoelectric pump is designed and simulated with different types of controllers and glucose-insulin models. COMSOL Multiphysics is used for piezoelectric-fluid-structural coupled 3D finite element simulations of the pump. Then, a reduced-order model (ROM) is simulated in MATLAB/Simulink together with optimal and proportional-integral-derivative (PID) controllers and glucose–insulin models of Ackerman, Bergman, and Sorensen. Divergence angle, nozzle/diffuser diameters, lengths, chamber height, excitation voltage, and frequency are optimized with dimensional constraints to achieve a high net flow rate and low power consumption. A prototype is manufactured and experimented with different excitation frequencies. It is shown that the proposed system successfully controls the delivered insulin for all three glucose–insulin models.
Mesial Rotation of the Upper First Molar: Association with Anterior Dental Crowding in Mixed and Permanent Dentition
Authors: Quinzi Vincenzo , Tecco Simona , Nota Alessandro , Caggiati Eleonora , Mummolo Stefano , Marzo Giuseppe
Published: July 2020

The position of the upper first molar (UFM) is currently considered the “key of occlusion”. The purpose of this study was to evaluate the prevalence of the mesiorotated UFM, and its relationship with anterior dental crowding in mixed and permanent dentition. Intra-oral scans of upper dental arches of 180 subjects with mixed dentition and 169 subjects with permanent dentition were retrospectively analyzed to investigate the presence of UFM mesiopalatal rotation and presence of anterior crowding. The occlusal relationship and the presence of caries were also recorded. In subjects with permanent dentition, there was a statistically significant correlation between anterior crowding and UFM mesiopalatal rotation (Pearson’s chi-square = 9.03; p = 0.06). Among cases with permanent dentition, subjects with UFM mesiopalatal rotation showed an OR = 3.28 (95% CI = 0.99–10.93; p = 0.042) of moderate/severe anterior dental crowding, respect to subjects without UFM rotation. In subjects with mixed dentition, there was a statistically significant correlation between molar occlusal relationship and UFM mesiopalatal rotation (Pearson’s chi-square = 14.98; p = 0.002), and subjects with molar class II showed a OR = 10.2 (95% CI = 2.16–48.22; p = 0.0005) to present UFM mesiopalatal rotation, with respect to subjects with molar class I. UFM mesiopalatal rotation is associated to anterior dental crowding in permanent dentition, and to class II malocclusion in mixed dentition.
Prediction of Antimalarial Drug-Decorated Nanoparticle Delivery Systems with Random Forest Models
Authors: Urista Diana V. , Carrué Diego B. , Otero Iago , Arrasate Sonia , Quevedo-Tumailli Viviana F. , Gestal Marcos , González-Díaz Humbert , Munteanu Cristian R.
Published: July 2020

Drug-decorated nanoparticles (DDNPs) have important medical applications. The current work combined Perturbation Theory with Machine Learning and Information Fusion (PTMLIF). Thus, PTMLIF models were proposed to predict the probability of nanoparticle–compound/drug complexes having antimalarial activity (against Plasmodium). The aim is to save experimental resources and time by using a virtual screening for DDNPs. The raw data was obtained by the fusion of experimental data for nanoparticles with compound chemical assays from the ChEMBL database. The inputs for the eight Machine Learning classifiers were transformed features of drugs/compounds and nanoparticles as perturbations of molecular descriptors in specific experimental conditions (experiment-centered features). The resulting dataset contains 107 input features and 249,992 examples. The best classification model was provided by Random Forest, with 27 selected features of drugs/compounds and nanoparticles in all experimental conditions considered. The high performance of the model was demonstrated by the mean Area Under the Receiver Operating Characteristics (AUC) in a test subset with a value of 0.9921 ± 0.000244 (10-fold cross-validation). The results demonstrated the power of information fusion of the experimental-centered features of drugs/compounds and nanoparticles for the prediction of nanoparticle–compound antimalarial activity. The scripts and dataset for this project are available in the open GitHub repository.
Instrumentation-Free Semiquantitative Immunoanalysis Using a Specially Patterned Lateral Flow Assay Device
Authors: Lee Kyung Won , Yu Ye Chan , Chun Hyeong Jin , Jang Yo Han , Han Yong Duk , Yoon Hyun C.
Published: July 2020

In traditional colorimetric lateral flow immunoassay (LFI) using gold nanoparticles (AuNPs) as a probe, additional optical transducers are required to quantify the signal intensity of the test line because it presents as a single red-colored line. In order to eliminate external equipment, the LFI signal should be quantifiable by the naked eye without the involvement of optical instruments. Given this objective, the single line test zone of conventional LFI was converted to several spots that formed herringbone patterns. When the sandwich immunoassay was performed on a newly developed semi-quantitative (SQ)-LFI system using AuNPs as an optical probe, the spots were colorized and the number of colored spots increased proportionally with the analyte concentration. By counting the number of colored spots, the analyte concentration can be easily estimated with the naked eye. To demonstrate the applicability of the SQ-LFI system in practical immunoanalysis, microalbumin, which is a diagnostic marker for renal failure, was analyzed using microalbumin-spiked artificial urine samples. Using the SQ-LFI system, the calibration results for artificial urine-based microalbumin were studied, ranging from 0 to 500 μg/mL, covering the required clinical detection range, and the limit of detection (LOD) value was calculated to be 15.5 μg/mL. Thus, the SQ-LFI system provides an avenue for the realization of an efficient quantification diagnostic device in resource-limited conditions.
Vividness, Consciousness and Mental Imagery: A Start on Connecting the Dots
Authors: D’Angiulli Amedeo
Published: July 2020

Over twenty years ago, Baars [...]
Molecular Bases of Mechanisms Accounting for Drug Resistance in Gastric Adenocarcinoma
Authors: Marin Jose J. G. , Perez-Silva Laura , Macias Rocio I. R. , Asensio Maitane , Peleteiro-Vigil Ana , Sanchez-Martin Anabel , Cives-Losada Candela , Sanchon-Sanchez Paula , Sanchez De Blas Beatriz , Herraez Elisa , Briz Oscar , Lozano Elisa
Published: July 2020

Gastric adenocarcinoma (GAC) is the most common histological type of gastric cancer, the fifth according to the frequency and the third among the deadliest cancers. GAC high mortality is due to a combination of factors, such as silent evolution, late clinical presentation, underlying genetic heterogeneity, and effective mechanisms of chemoresistance (MOCs) that make the available antitumor drugs scarcely useful. MOCs include reduced drug uptake (MOC-1a), enhanced drug efflux (MOC-1b), low proportion of active agents in tumor cells due to impaired pro-drug activation or active drug inactivation (MOC-2), changes in molecular targets sensitive to anticancer drugs (MOC-3), enhanced ability of cancer cells to repair drug-induced DNA damage (MOC-4), decreased function of pro-apoptotic factors versus up-regulation of anti-apoptotic genes (MOC-5), changes in tumor cell microenvironment altering the response to anticancer agents (MOC-6), and phenotypic transformations, including epithelial-mesenchymal transition (EMT) and the appearance of stemness characteristics (MOC-7). This review summarizes updated information regarding the molecular bases accounting for these mechanisms and their impact on the lack of clinical response to the pharmacological treatment currently used in GAC. This knowledge is required to identify novel biomarkers to predict treatment failure and druggable targets, and to develop sensitizing strategies to overcome drug refractoriness in GAC.
Potentiality, Limitations, and Consequences of Different Experimental Models to Improve Photodynamic Therapy for Cancer Treatment in Relation to Antiangiogenic Mechanism
Authors: Majerník Martin , Jendželovský Rastislav , Fedoročko Peter
Published: July 2020

The relevance of experimentally gained information represents a long-term debating issue in the field of molecular biology research. The loss of original conditions in the in vitro environment affects various biological mechanisms and cellular interactions. Consequently, some biochemical mechanisms are lost or critically altered. Analyses in these modified conditions could, therefore, distort the relevancy of experimentally gained information. In some cases, the similarities with original conditions are so small that utilization of simpler in vitro models seems impossible, or could occur in a very limited way. To conclude, the study of more complex phenomena places higher demands on the complexity of the experimental model. The latest information highlights the fact that the tumor angiogenesis mechanism has very complex features. This complexity can be associated with a wide range of angiogenic factors expressed by a variety of malignant and non-malignant cells. Our article summarizes the results from various experimental models that were utilized to analyze a photodynamic therapy effect on tumor angiogenic mechanisms. Additionally, based on the latest information, we present the most important attributes and limitations of utilized experimental models. We also evaluate the essential problems associated with angiogenic mechanism induction after photodynamic therapy application.
Structural Elucidation of Enantiopure and Racemic 2-Bromo-3-Methylbutyric Acid
Authors: Seidel Rüdiger W. , Nöthling Nils , Goddard Richard , Lehmann Christian W.
Published: July 2020

Halogenated carboxylic acids have been important compounds in chemical synthesis and indispensable research tools in biochemical studies for decades. Nevertheless, the number of structurally characterized simple α-brominated monocarboxylic acids is still limited. We herein report the crystallization and structural elucidation of (R)- and rac-2-bromo-3-methylbutyric acid (2-bromo-3-methylbutanoic acid, 1) to shed light on intermolecular interactions, in particular hydrogen bonding motifs, packing modes and preferred conformations in the solid-state. The crystal structures of (R)- and rac-1 are revealed by X-ray crystallography. Both compounds crystallize in the triclinic crystal system with Z = 2; (R)-1 exhibits two crystallographically distinct molecules. In the crystal, (R)-1 forms homochiral O–H···O hydrogen-bonded carboxylic acid dimers with approximate non-crystallographic C2 symmetry. In contrast, rac-1 features centrosymmetric heterochiral dimers with the same carboxy syn···syn homosynthon. The crystal packing of centrosymmetric rac-1 is denser than that of its enantiopure counterpart (R)-1. The molecules in both crystal structures adopt a virtually identical staggered conformation, despite different crystal environments, which indicates a preferred molecular structure of 1. Intermolecular interactions apart from classical O–H···O hydrogen bonds do not appear to have a crucial bearing on the solid-state structures of (R)- and rac-1.
Evaluation of Surface Characteristics and Cytotoxicity of Dental Composites
Authors: Crăciun Antarinia , Bȃldea Ioana , Ispas Ana , Badea Mîndra Eugenia , Petean Ioan , Sarosi Codruta , Moldovan Marioara , Cuc Stanca , Ene Razvan , Crişan Maria
Published: July 2020

The purpose of this study was to evaluate the surface and in vitro cytotoxicity on human dysplastic oral keratinocytes (DOK) of four commercial resins-based dental composites commonly used in prosthodontics dental therapies: two indirect composites for crown and bridges—SR Adoro (IvoclarVivadent GmbH) and Solidex (Shofu Dental GmbH); and two dual-curing luting resin cements—RelyxUnicem (3M ESPE Dental Products) and Variolink Esthetic DC (IvoclarVivadentGmbH). A complex assessment of surface characteristics of the four materials was conducted before and after the exposure to artificial saliva through various analyses, such as Scanning Electron Microscopy, Atomic Force Microscopy and Cross Polarized Light Microscopy (PLM). The results showed that DOK viability was not severely affected by exposure to any of these materials; however, Variolink expressed higher values but still above the toxicity level of the rest of the composites. The analysis of the surface structure between initial and artificial saliva exposed specimens returned a compact aspect in both categories and although Variolink and Relyx were subjected to increased roughness after saliva exposure, no damage of the internal compactness was recorded, demonstrating a fair behavior of the luting cements in contact with the saliva.
Vibrational Dynamics of a Chiral Smectic Liquid Crystal Undergoing Vitrification and Cold Crystallization
Authors: Drzewicz Anna , Juszyńska-Gałązka Ewa , Zając Wojciech , Kula Przemysław
Published: July 2020

Vibrational dynamics in the glass transition and the cold crystallization process of (S)-4′-(1-methyloctyloxycarbonyl) biphenyl-4-yl 4-[7-(2,2,3,3,4,4,4-heptafluorobutoxy) heptyl-1-oxy]-benzoate (3F7HPhH7) was studied by Fourier transform infrared spectroscopy (FTIR) during cooling/heating experimental runs. The measured spectra processing was supported by quantum chemical density functional theory (DFT) calculations (frequency assignments). The perturbation-correlation moving window two-dimensional analysis (PCMW2D) was performed to examine how the height of individual absorption bands change under with temperature. Two-dimensional correlation analysis (2D-COS) was used to detect freezing-in or activation of the stochastic movements during the vitrification and the cold crystallization processes. Upon cooling, the vitrification process involves freezing-in of the stochastic movements of ester groups. Upon heating, as the cold crystallization process begins, the first to respond are the vibrations of the C–O–C and C=O groups in the rigid core.
Increased Genetic Diversity via Gene Flow Provides Hope for Acacia whibleyana, an Endangered Wattle Facing Extinction
Authors: Blyth Colette , Christmas Matthew J. , Bickerton Doug C. , Faast Renate , Packer Jasmin G. , Lowe Andrew J. , Breed Martin F.
Published: July 2020

In this paper we apply a conservation genomics approach to make evidence-based management recommendations for Acacia whibleyana, an endangered shrub endemic to Eyre Peninsula, South Australia. We used population genomic analysis to assess genetic connectivity, diversity, and historical inbreeding across all known stands of the species sampling remnant stands, revegetated stands of unknown origin, and a post-fire seedling cohort. Our results indicate a degree of historical connectivity across the landscape, but habitat loss and/or pollinator community disruption are potential causes of strong genetic structure across the remnant stands. Remnant stands had low genetic diversity and showed evidence of historical inbreeding, but only low levels of intra-stand relatedness indicating that risks of contemporary inbreeding are low. Analysis of a post-fire first generation cohort of seedlings showed they likely resulted from intra-stand matings, resulting in reduced genetic diversity compared to the parents. However, admixed seedlings in this cohort showed an increase in heterozygosity relative to likely sources and the non-admixed seedlings of the same stand. Assisted inter-stand gene flow may prove an effective management strategy to boost heterozygosity and corresponding increases in adapting capacity in this endangered species.
COVID-19-Related Coagulopathy—Is Transferrin a Missing Link?
Authors: McLaughlin Katie-May , Bechtel Marco , Bojkova Denisa , Münch Christian , Ciesek Sandra , Wass Mark N. , Michaelis Martin , Cinatl Jindrich
Published: July 2020

SARS-CoV-2 is the causative agent of COVID-19. Severe COVID-19 disease has been associated with disseminated intravascular coagulation and thrombosis, but the mechanisms underlying COVID-19-related coagulopathy remain unknown. The risk of severe COVID-19 disease is higher in males than in females and increases with age. To identify gene products that may contribute to COVID-19-related coagulopathy, we analyzed the expression of genes associated with the Gene Ontology (GO) term “blood coagulation” in the Genotype-Tissue Expression (GTEx) database and identified four procoagulants, whose expression is higher in males and increases with age (ADAMTS13, F11, HGFAC, KLKB1), and two anticoagulants, whose expression is higher in females and decreases with age (C1QTNF1, SERPINA5). However, the expression of none of these genes was regulated in a proteomics dataset of SARS-CoV-2-infected cells and none of the proteins have been identified as a binding partner of SARS-CoV-2 proteins. Hence, they may rather generally predispose individuals to thrombosis without directly contributing to COVID-19-related coagulopathy. In contrast, the expression of the procoagulant transferrin (not associated to the GO term “blood coagulation”) was higher in males, increased with age, and was upregulated upon SARS-CoV-2 infection. Hence, transferrin warrants further examination in ongoing clinic-pathological investigations.
Updating the Risk Stratification for Sudden Cardiac Death in Cardiomyopathies: The Evolving Role of Cardiac Magnetic Resonance Imaging. An Approach for the Electrophysiologist
Authors: Kariki Ourania , Antoniou Christos-Konstantinos , Mavrogeni Sophie , Gatzoulis Konstantinos A.
Published: July 2020

The prevention of sudden cardiac death (SCD) in cardiomyopathies (CM) remains a challenge. The current guidelines still favor the implantation of devices for the primary prevention of SCD only in patients with severely reduced left ventricular ejection fraction (LVEF) and heart failure (HF) symptoms. The implantation of an implantable cardioverter-defibrillator (ICD) is a protective barrier against arrhythmic events in CMs, but the benefit does not outweigh the cost in low risk patients. The identification of high risk patients is the key to an individualized prevention strategy. Cardiac magnetic resonance (CMR) provides reliable and reproducible information about biventricular function and tissue characterization. Furthermore, late gadolinium enhancement (LGE) quantification and pattern of distribution, as well as abnormal T1 mapping and extracellular volume (ECV), representing indices of diffuse fibrosis, can enhance our ability to detect high risk patients. CMR can also complement electro-anatomical mapping (EAM), a technique already applied in the risk evaluation and in the ventricular arrhythmias ablation therapy of CM patients, providing a more accurate assessment of fibrosis and arrhythmic corridors. As a result, CMR provides a new insight into the pathological substrate of CM. CMR may help identify high risk CM patients and, combined with EAM, can provide an integrated evaluation of scar and arrhythmic corridors in the ablative therapy of ventricular arrhythmias.
Prenatal Management of Congenital Human Cytomegalovirus Infection in Seropositive Pregnant Patients Treated with Azathioprine
Authors: Cavoretto Paolo Ivo , Fornara Chiara , Baldoli Cristina , Arossa Alessia , Furione Milena , Candiani Massimo , Rovere Querini Patrizia , Barera Graziano , Poloniato Antonella , Gaeta Gerarda , Spinillo Arsenio , Lilleri Daniele
Published: July 2020

Human cytomegalovirus (HCMV) is the leading infectious agent causing congenital disabilities. The risk of HCMV transmission to the fetus in pregnant women receiving immunosuppressive agents is unknown. We describe two cases of pregnant women with evidence of pre-conception HCMV protective immunity receiving azathioprine for ulcerative colitis or systemic lupus erythematosus. Both women reactivated the HCMV and transmitted the infection to the fetuses. One newborn showed unilateral hearing deficits and brain abnormalities while the other was asymptomatic. The mother of the symptomatic newborn had low levels of total and HCMV-specific blood CD4+ T cells. Women receiving immunosuppressive agents deserve information about the risk of HCMV congenital infection and should be monitored for HCMV infection during pregnancy. Their newborns should be screened for HCMV congenital infection.
Aldosterone-Mediated Sodium Retention Is Reflected by the Serum Sodium to Urinary Sodium to (Serum Potassium)2 to Urinary Potassium (SUSPPUP) Index
Authors: Kanaan Evelien , Haase Matthias , Vonend Oliver , Reincke Martin , Schott Matthias , Willenberg Holger S.
Published: July 2020

The serum sodium to urinary sodium ratio divided by the (serum potassium)2 to urinary potassium ratio (SUSPPUP formula) reflects aldosterone action. We here prospectively investigated into the usefulness of the SUSPPUP ratio as a diagnostic tool in primary hyperaldosteronism. Parallel measurements of serum and urinary sodium and potassium concentrations (given in mmol/L) in the fasting state were done in 225 patients. Of them, 69 were diagnosed with primary aldosteronism (PA), 102 with essential hypertension (EH), 26 with adrenal insufficiency (AI) and 28 did not suffer from the above-mentioned disorders and were assigned to the reference group (REF). The result of the SUSPPUP formula was highest in the PA group (7.4, 4.2–12.3 L/mmol), followed by EH (3.2, 2.3–4.3 L/mmol), PA after surgery (3.9, 3.0–6.0 L/mmol), REF (3.4 ± 1.4 L/mmol) and AI (2.9 +/− 1.2 L/mmol). The best sensitivity in distinguishing PA from EH was reached by multiplication of the aldosterone to renin-ratio (ARR) with the SUSPPUP formula (92.7% at a cut off > 110 L/mmol), highest specificity was reached by the SUSPPUP determinations (87.2%). The integration of the SUSPPUP ratio into the ARR helps to improve the diagnosis of hyperaldosteronism substantially.
Evaluation Method of Heavy-Ion-Induced Single-Event Upset in 3D-Stacked SRAMs
Authors: Zhao Peixiong , Liu Tianqi , Cai Chang , He Ze , Li Dongqing , Liu Jie
Published: July 2020

The interaction of radiation with three-dimensional (3D) electronic devices can be determined through the detection of single-event effects (SEU). In this study, we propose a method for the evaluation of SEUs in 3D static random-access memories (SRAMs) induced by heavy-ion irradiation. The cross-sections (CSs) of different tiers, as a function of the linear energy transfer (LET) under high, medium, and low energy heavy-ion irradiation, were obtained through Monte Carlo simulations. The simulation results revealed that the maximum value of the CS was obtained under the medium-energy heavy-ion penetration, and the effect of penetration range of heavy ions was observed in different tiers of 3D-stacked devices. The underlying physical mechanisms of charge collection under different heavy-ion energies were discussed. Thereafter, we proposed an equation of the critical heavy-ion range that can be used to obtain the worst CS curve was proposed. Considering both the LET spectra and flux of galactic cosmic ray (GCR) and the variation in the heavy-ion Bragg peak values with the atomic number, we proposed a heavy-ion irradiation test guidance for 3D-stacked devices. In addition, the effectiveness of this method was verified through simulations of the three-tier vertically stacked SRAM and the ultrahigh-energy heavy-ion irradiation experiment of the two-tier vertically stacked SRAM. this study provides a theoretical framework for the detection of SEUs induced by heavy-ion irradiation in 3D-integrated devices.
VaultPoint: A Blockchain-Based SSI Model that Complies with OAuth 2.0
Authors: Hong Seongho , Kim Heeyoul
Published: July 2020

An identity management including authentication and authorization in a network environment is a critical security factor. Various models for identity management have been developed continually, from the silo model to the federated model and to the recently introduced self-sovereign identity (SSI) model. In particular, SSI makes users manage their own information by themselves independently of any organizations. SSI utilizes the newly emerged blockchain technology and many studies of it are in progress. However, SSI has not had wide public use because of its low compatibility and inconvenience. This is because it involves an unfamiliar user experience and an immature process. To solve this problem, this paper proposes a new blockchain-based SSI model that complies with the popular and mature standard of OAuth 2.0. Using blockchain, the proposed model secures users’ data sovereignty where users can use and control their own information in a decentralized manner, instead of depending on a specific monopolistic service-providers. Users and clients who are familiar with the existing OAuth can easily accept the proposed model and apply it, which makes both usability and scalability of the model excellent. This paper confirmed the feasibility of the proposed model by implementing it and a security analysis was performed. The proposed model is expected to contribute to the expansion of both blockchain technology and SSI.
Field Oriented Control-Based Reduction of the Vibration and Power Consumption of a Blood Pump
Authors: Golesorkhie Farya , Yang Fuwen , Vlacic Ljubo , Tansley Geoff
Published: July 2020

Power quality and energy efficiency are of great importance in motor control. The motor of any medical device needs to have a smooth torque and minimal vibration in order to maximise its energy efficiency and patient comfort. Furthermore, in rotary blood pumps, excessive energy wasted due to vibration is converted into uncontrolled movement of the mechanical parts and thus could reduce the life of the motor-pump. Besides mechanical or hydraulic origin, one of the causes of vibration in any pump is torque ripple resulting from motor phase commutation. In this paper, using relevant equipment, two extreme scenarios were examined for vibration and electrical efficiency comparison due to power quality in a blood pump: one trapezoidal control with a trapezoidal phase current output; the other a field oriented control (FOC) with a non-distorted sinusoidal phase current. The test motor-pump was the Arteriovenous Fistula Eligibility (AFE) System that is used prior to haemodialysis. The trapezoidal technique was implemented utilising the Allegro a4941 fan driver (Allegro Microsystem, 2012), and the FOC technique was implemented using the Texas Instrument digital signal processor (TMS320F28335). The aim was to reduce the energy wasted over vibration, and to achieve smooth operation of the AFE System. Vibration was measured with a one-axis accelerometer; results showed considerably lower vibration due to less current ripple associated with the FOC control as well as lower power consumption.