bluuebunny/crossref_metadata_2025 huggingface dataset:
Impacts of the Pandemic, Animal Source Food Retailers’ and Consumers’ Knowledge and Attitudes toward COVID-19, and Their Food Safety Practices in Chiang Mai, Thailand
Authors: Jainonthee Chalita , Dang-Xuan Sinh , Nguyen-Viet Hung , Unger Fred , Chaisowwong Warangkhana
Published: August 2022

The COVID-19 pandemic affected the food supply chain, retailers, and consumers owing to infection awareness. This study evaluated the impacts COVID-19 on ASF retailers’ businesses and consumers’ livelihoods, as well as their knowledge toward the disease, attitudes, and food safety practices to prevent infections. The study includes a cross-sectional component that was conducted in urban/peri-urban (U/PU) and rural areas in Chiang Mai province. In another part of the study, a structured questionnaire was developed for animal source food (ASF) retailers and consumers, with three primary parts for data analysis: general information, COVID-19 impacts, and knowledge, attitudes, and practices (KAP) assessment. Data corresponding to three periods of interest (before the COVID-19 outbreak, during partial lockdown, and present) were gathered and analyzed. In this study, 155 retailers and 150 consumers participated, of which the majority of the respondents were female (70.3% and 82.7%, respectively) with average ages of 47.4 and 44.9 years, respectively. The most noticeable effect of COVID-19 was a decline in income for retailers and consumers. The KAP scores of consumers in both areas were not significantly different, whereas the retailer attitudes toward COVID-19 prevention and food safety practices scored more highly in rural areas than in U/PU. During the partial lockdown, food safety practices significantly improved relative to the time preceding the outbreak, and these practices have remained constant to the present day. The results revealed that gender, age group, business type, and type of ASF retailers were associated with the KAP of the retailers, whereas gender, age group, education, number of family members, and occupation were associated with the KAP of the consumers. Our findings provide in-depth information about the effects of COVID-19 on ASF retailers and consumers, as well as their KAP regarding the outbreak and food safety, which may serve as support in developing policies for improved health and food safety.
COVID-19 Vaccination Intentions amongst Healthcare Workers: A Scoping Review
Authors: Willems Lucia D. , Dyzel Vernandi , Sterkenburg Paula S.
Published: August 2022

A worldwide vaccination programme is the chosen strategy against the COVID-19 pandemic. Vaccine hesitancy, however, forms a threat to achieving a high degree of vaccination. Healthcare workers (HCWs) are exposed to greater risks, in addition to HCWs who care for people with intellectual disabilities (ID). However, little is still known about these groups’ vaccine hesitancy. This review aims to provide insight into the intentions and attitudes of HCWs on COVID-19 vaccination, including those who care for people with ID. The search included both types and was conducted in nine databases. A total of 26 papers were identified concerning the vaccine intentions of 43,199 HCWs worldwide. The data were gathered both quantitively and qualitatively. The papers were analysed for all of the themes regarding vaccine intentions, which were: (1) percentages of vaccine willingness; (2) predictors of willingness; (3) attitudes of willingness and hesitancy; (4) sources of vaccination information; (5) contextual factors and changes in COVID-19 vaccine acceptance over time; and (6) future strategies for interventions. Concerns about vaccine safety, efficacy and short- and long-term side effects were the most prominent in HCWs and, therefore, should be addressed in future intervention strategies. Furthermore, interactive interventions are recommended to facilitate exchange, and accurate information should be accessible to target groups on social media platforms.
Improving Tractor Safety: A Comparison between the Usability of a Conventional and Enhanced Rear-Mounted Foldable ROPS (FROPS)
Authors: Vigoroso Lucia , Caffaro Federica , Micheletti Cremasco Margherita , Cavallo Eugenio
Published: August 2022

Tractor rollover is the main cause of both fatal and non-fatal injuries in agriculture. The foldable rollover protective structure (FROPS) can help to prevent these injuries. However, in many cases, the FROPS is left in a folded-down position. Human factor and rear-mounted FROPS technical characteristics influence operators’ behavior in roll-bar handling. To improve the FROPS’s comfortable use, the prototype of an enhanced handling system was developed, and its usability was tested and compared with a conventional FROPS. Usability was assessed with 93 novice tractor users through an ad hoc questionnaire (investigating perceived effort, time demand, the posture adopted and satisfaction) and observations (investigating effectiveness and efficiency) during lowering and raising tasks. For both tasks, the participants perceived significantly less effort, less physical discomfort, a higher level of satisfaction and less time demand while operating the enhanced FROPS. Observations showed that the critical issues that emerged for the conventional FROPS were eliminated by adopting the developed and implemented handling system. The developed handling system showed itself to be usable and effective in making the FROPS easier and safer to be operated.
Winning the Blue Sky Defense War: Assessing Air Pollution Prevention and Control Action Based on Synthetic Control Method
Authors: Zhang Haotian , Sun Xiumei , Wang Xueyang , Yan Su
Published: August 2022

Undoubtedly, the rapid development of urbanization and industrialization in China has led to environmental problems, among which air pollution is particularly prominent. In response, the Chinese government has introduced a series of policies, including the Air Pollution Control and Prevention Action Plan (APPA), which is one of the most stringent environmental regulations in history. The scientific evaluation of the implementation of this regulation is important for China to win the battle of blue sky. Therefore, this study uses a synthetic control method to explore the effects of APPA on air pollution (AP) based on data of 30 provinces from 2000 to 2019. The study concludes that (1) APPA significantly reduces AP in the treatment provinces, and subsequent robustness tests validate our findings. However, the persistence of the policy effect is short in some provinces, and the rate of AP reduction slows down or even rebounds in the later stages of the policy. (2) The reduction effect of APPA varies significantly between regions and provinces. (3) The results of mechanism tests show that APPA reduces AP through high-quality economic development, population agglomeration, control of carbon emissions, and optimization of energy structure. Based on the above findings, targeted recommendations are proposed to promote AP control in China and win the blue sky defense war.
Development of a Combined Exercise and Cognitive Stimulation Intervention for People with Mild Cognitive Impairment—Designing the MEMO_MOVE PROGRAM
Authors: Rondão Catarina , Mota Maria , Esteves Dulce
Published: August 2022

Dementia patients are at high risk for the decline of both physical and cognitive capacities, resulting in an increased risk of the loss of autonomy. Exercise is regarded as a non-pharmacological therapy for dementia, considering the potential benefits of preventing cognitive decline and improving physical fitness. In this paper, we aim to describe the different design stages for an exercise program combined with cognitive stimulation for a population with mild cognitive impairment, i.e., the MEMO_MOVE program. Methods: The intervention design followed the Medical Research Council’s guidelines for complex interventions and was structured according to the six steps in quality intervention development (6SQuID). The intervention was described considering the Template for Intervention Description and Replication (TIDieR). In order to establish the intervention characteristics, a literature review was conducted to collate and analyze previous work, which provided a summary the type of exercise that should be implemented among this population. Results: The MEMO_MOVE program was structured and described, regarding (i) inclusion of a cognitive stimulation component; (ii) the kind of cognitive stimulation; and (iii) the type of exercise, duration, frequency, intensity, and program length. Conclusions: A systematic step-by-step process design was followed to create a specific intervention to promote physical fitness and cognitive stimulation in individuals with mild dementia.
The Perception of Lactose-Related Symptoms of Patients with Lactose Malabsorption
Authors: Di Stefano Michele , Brondino Natascia , Bonaso Vera , Miceli Emanuela , Lapia Francesco , Grandi Giacomo , Pagani Elisabetta , Corazza Gino Roberto , Di Sabatino Antonio
Published: August 2022

Background: Dairy products are frequently considered responsible for post-prandial symptoms and are withdrawn from the diet, even against medical advice. We analysed the symptoms patients consider as lactose related; we also evaluated if psychological profile may affect the interpretation of the relationship between lactose and symptoms. Methods: In 268 patients undergoing lactose breath test, symptoms considered evoked by lactose intake were recorded and their severity measured. In the second part, symptom onset of 40 randomly selected patients was detected after both lactose and glucose breath test were blindly performed. Questionnaires evaluating anxiety, suggestibility and personality trait were administered. Key Results: Symptoms depending on functional gastrointestinal disorders or reflux disease were frequent in self-reported lactose-intolerant patients. In comparison with lactose malabsorption, these symptoms proved to be more frequent in patients with negative lactose breath test. The blinded administration of lactose and glucose demonstrated that a correct link between lactose intake and symptom onset was possible, only in 47.5% of the subjects, making this test inaccurate. None of the investigated psychological characteristics were different between patients with a nocebo response and patients not experiencing nocebo. Conclusions: Patients with self-reported lactose intolerance are frequently unaware about clinical presentation of this condition, and correct information is needed. The detection of symptom onset after lactose is an inaccurate test for lactose intolerance. Furthermore, the analysis of psychological characteristics of patients undergoing hydrogen breath test is not useful to select the subgroup at risk for a nocebo response. New strategies to diagnose lactose intolerance are mandatory.
Concentrations of Pb and Other Associated Elements in Soil Dust 15 Years after the Introduction of Unleaded Fuel and the Human Health Implications in Pretoria, South Africa
Authors: Olowoyo Joshua O. , Lion Ntebo , Unathi Tshoni , Oladeji Oluwaseun M.
Published: August 2022

Leaded fuel has been reported to contain certain amounts of toxic trace metals such as Pb and Cadmium (Cd), which may have negative impacts on humans and the environment. Unleaded fuel was introduced to South Africa in 2006 with the aim of reducing and eventually eliminating the negative impact of leaded fuel on the environment. However, trace metals are usually nonbiodegradable, and it may therefore be necessary to monitor their presence in the environment so as to evaluate their possible impact on human health. The present study evaluated the levels of Pb and other heavy metals in soil samples collected from petrol (gas) filling stations and from busy roads just around the filling stations in Pretoria, South Africa, fifteen years after the introduction of unleaded fuel. A total of twenty-four (24) soil samples were analysed for lead (Pb), chromium (Cr), copper (Cu), zinc (Zn), arsenic (As), iron (Fe), manganese (Mn), nickel (Ni), titanium (Ti), and cadmium (Cd) using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The results showed that the concentrations of the trace metals were in the following ranges: Pb, 0.08 ± 0.02–188.36 ± 15.32 ug/g; Mn, 5.35 ± 0.34–6842.43 ± 1.35 ug/g; Zn, 1.82 ± 0.22–9814.89 ± 22.32 ug/g; As, 0.21 ± 0.00–8.42 ± 2.44 ug/g; Cu, 10.51 ± 3.41–859 ± 0.09 ug/g; Cr, 5.80 ± 2.21–417.70 ± 9.08 ug/g; Ti, 19.94 ± 4.99–1036.12 ± 1.49 ug/g; and Fe, 3.06 ± 7.87–674.07 ± 12.22 mg/g. The highest concentrations from all the elements were recorded for Fe in all the collected soil samples. The concentrations of Pb in the soils collected from sites associated with high traffic and industrial areas were higher than for those from all other sites, and the differences were significant (p < 0.05). The pollution index (PI), which is the anthropogenic influence of the trace metals, and the geoaccumulation (Igeo), which allows for the removal of possible variations as regards the studied element in the soil due to the possible differences in the background value, showed that some samples were enriched anthropogenically. The PI for Ni, Pb, Cu, and Cr indicated highly anthropogenically contaminated soils, especially at sites associated with high traffic volumes and in industrial areas. The Igeo showed moderately polluted areas for Pb and Cu in high-traffic areas. The exposure routes for the toxic trace metals that were of concern in the study were either through ingestion or dermal contact. The calculated hazard quotient showed both noncarcinogenic and carcinogenic risks for Fe and Mn via ingestion and through dermal contact for both children and adults, respectively. The concentrations of Pb were high and similar to those that were previously reported in the study and pointed to vehicular emission as one of the contributors. The study also noticed an increase in the presence of Mn and Fe in all soil samples.
Patterns of Muscle-Related Risk Factors for Sarcopenia in Older Mexican Women
Authors: Carrillo-Vega María Fernanda , Pérez-Zepeda Mario Ulises , Salinas-Escudero Guillermo , García-Peña Carmen , Reyes-Ramírez Edward Daniel , Espinel-Bermúdez María Claudia , Sánchez-García Sergio , Parra-Rodríguez Lorena
Published: August 2022

Early detriment in the muscle mass quantity, quality, and functionality, determined by calf circumference (CC), phase angle (PA), gait time (GT), and grip strength (GSt), may be considered a risk factor for sarcopenia. Patterns derived from these parameters could timely identify an early stage of this disease. Thus, the present work aims to identify those patterns of muscle-related parameters and their association with sarcopenia in a cohort of older Mexican women with neural network analysis. Methods: Information from the functional decline patterns at the end of life, related factors, and associated costs study was used. A self-organizing map was used to analyze the information. A SOM is an unsupervised machine learning technique that projects input variables on a low-dimensional hexagonal grid that can be effectively utilized to visualize and explore properties of the data allowing to cluster individuals with similar age, GT, GSt, CC, and PA. An unadjusted logistic regression model assessed the probability of having sarcopenia given a particular cluster. Results: 250 women were evaluated. Mean age was 68.54 ± 5.99, sarcopenia was present in 31 (12.4%). Clusters 1 and 2 had similar GT, GSt, and CC values. Moreover, in cluster 1, women were older with higher PA values (p < 0.001). From cluster 3 upward, there is a trend of worse scores for every variable. Moreover, 100% of the participants in cluster 6 have sarcopenia (p < 0.001). Women in clusters 4 and 5 were 19.29 and 90 respectively, times more likely to develop sarcopenia than those from cluster 2 (p < 0.01). Conclusions: The joint use of age, GSt, GT, CC, and PA is strongly associated with the probability women have of presenting sarcopenia.
Exploring Occupational Health and Safety Standards Compliance in the South African Mining Industry, Limpopo Province, Using Principal Component Analysis
Authors: Muthelo Livhuwani , Mothiba Tebogo Maria , Malema Nancy Rambelani , Mbombi Masenyani Oupa , Mphekgwana Peter Modupi
Published: August 2022

The health and safety of the miners in the South African mining industry are guided by the regulations and standards applied to promote a healthy work environment. The miners must comply with these regulations/standards to protect themselves from potential occupational health and safety risks, accidents, and fatalities. The status of compliance to safety regulations and standards in the mining industry of Limpopo Province has received little attention from scholars. This study explores the practices related to occupational health and safety standards compliance in the mining industry. A total of 277 miners were randomly selected from 1300 respondents in the mining industry. Data were collected using a 31-item survey questionnaire, administered to miners to explore occupational health and safety standards compliance from December 2019 to May 2020. Principal Component Analysis (PCA) extracted key attributes of occupational health and safety standards compliance in the mining industry and uncovered relationships between different dimensions. The study revealed that seven factors could measure occupational health and safety standards practices. It was observed that Factor 1 (occupational health practice related to regulations) is correlated with Factor 2 (measures to reduce risk of injuries/accidents). Additionally, Factor 2 (measures to minimise the risk of injuries/accidents) is correlated with Factor 4 (impact of the environment and production). There is a correlation between non-compliance with the safety regulations and the occurrence of injuries and accidents.
Metabolomic Analysis Revealed Distinct Physiological Responses of Leaves and Roots to Huanglongbing in a Citrus Rootstock
Authors: Chen Qing , Min Ailing , Luo Shu , He Jinwei , Wu Runqin , Lin Ximeng , Wang Yan , He Wen , Zhang Yunting , Lin Yuanxiu , Li Mengyao , Zhang Yong , Luo Ya , Tang Haoru , Wang Xiaorong
Published: August 2022

Huanglongbing (HLB) is an obstinate disease in the citrus industry. No resistant citrus resources were currently available, but various degrees of Huanglongbing tolerance exist in different germplasm. Citrus junos is emerging as one of the popular rootstocks widely used in the citrus production. However, its responses to the HLB causal agent, Candidatus Liberibacter asiaticus (CLas), were still elusive. In the current study, we investigated the physiological, anatomical, and metabolomic responses of a C. junos rootstock ‘Pujiang Xiangcheng’ by a controlled CLas grafting inoculation. The summer flushes and roots were impaired at 15 weeks after inoculation, although typical leaf symptomatic phenotypes were not obvious. The chlorophyll pigments and the photosynthetic rate were compromised. The phloem sieve tubes were still working, despite the fact that the callose was deposited and the starch granules were accumulated in the phloem cells. A wide, targeted metabolomic analysis was carried out to explore the systematic alterations of the metabolites at this early stage of infection in the leaves and root system. The differentially accumulated metabolites in the CLas-affected leaves and roots compared with the mock-inoculation control tissues revealed that distinct responses were obvious. Besides the commonly observed alteration of sugar and amino acids, the active break down of starch in the roots was discovered. The different types of fatty acids were altered in the two tissues, with a more pronounced content decline in the roots. Our results not only provided fundamental knowledge about the response of the C. junos rootstock to the HLB disease, but also presented new insights into the host–pathogen interaction in the early stages.
Conformational Variability in Ground-State CFTR Lipoprotein Particle Cryo-EM Ensembles
Authors: Aleksandrov Luba A. , Aleksandrov Adrei A. , Jensen Timothy J. , Strauss Joshua D. , Fay Jonathan F.
Published: August 2022

Cystic fibrosis transmembrane regulator (CFTR) is a dynamic membrane protein belonging to the ABC transporter family. It is unusual within this family as it is an ion channel, as opposed to a transporter. Activation of CFTR requires ATP and phosphorylation by PKA, and dysregulation of CFTR mediated salt and water homeostasis can lead to cystic fibrosis. Recent advancements in structural biological methods have led to more than 10 published CFTR structures, and, so far, all of these structures of CFTR, determined by cryo-EM, have been limited to detergent-purified protein preparations. To visualize CFTR in an environment that more closely represents its native membranous environment, we utilized two different lipoprotein particle encapsulation techniques: one in which the ion channel is first purified and then reconstituted using the membrane scaffolding protein Saposin A and another that uses the solubilizing polymer Sokalan CP9 (DIBMA) to extract CFTR directly from membranes. Structures derived from these types of preparations may better correlate to their function, for instance, the single-channel measurements from membrane vesicles.
Targeted Next-Generation Sequencing of Congenital Hypothyroidism-Causative Genes Reveals Unexpected Thyroglobulin Gene Variants in Patients with Iodide Transport Defect
Authors: Bernal Barquero Carlos Eduardo , Geysels Romina Celeste , Jacques Virginie , Carro Gerardo Hernán , Martín Mariano , Peyret Victoria , Abregú María Celeste , Papendieck Patricia , Masini-Repiso Ana María , Savagner Frédérique , Chiesa Ana Elena , Citterio Cintia E. , Nicola Juan Pablo
Published: August 2022

Congenital iodide transport defect is an uncommon autosomal recessive disorder caused by loss-of-function variants in the sodium iodide symporter (NIS)-coding SLC5A5 gene and leading to dyshormonogenic congenital hypothyroidism. Here, we conducted a targeted next-generation sequencing assessment of congenital hypothyroidism-causative genes in a cohort of nine unrelated pediatric patients suspected of having a congenital iodide transport defect based on the absence of 99mTc-pertechnetate accumulation in a eutopic thyroid gland. Although, unexpectedly, we could not detect pathogenic SLC5A5 gene variants, we identified two novel compound heterozygous TG gene variants (p.Q29* and c.177-2A>C), three novel heterozygous TG gene variants (p.F1542Vfs*20, p.Y2563C, and p.S523P), and a novel heterozygous DUOX2 gene variant (p.E1496Dfs*51). Splicing minigene reporter-based in vitro assays revealed that the variant c.177-2A>C affected normal TG pre-mRNA splicing, leading to the frameshift variant p.T59Sfs*17. The frameshift TG variants p.T59Sfs*17 and p.F1542Vfs*20, but not the DUOX2 variant p.E1496Dfs*51, were predicted to undergo nonsense-mediated decay. Moreover, functional in vitro expression assays revealed that the variant p.Y2563C reduced the secretion of the TG protein. Our investigation revealed unexpected findings regarding the genetics of congenital iodide transport defects, supporting the existence of yet to be discovered mechanisms involved in thyroid hormonogenesis.
Molecular Dynamics Simulation of Coiled Carbon Nanotube Pull-Out from Matrix
Authors: Huang Feng , Zhou Shuai
Published: August 2022

The interaction between coiled carbon nanotubes (CCNT) and the polymer matrix is important in the mechanical, thermal, and electrical properties of the CCNT reinforced nanocomposite. In this study, molecular dynamics (MD) simulations were performed to study the interfacial characteristics of polymer nanocomposites (PNCs). Furthermore, the influence of the geometries of the CCNTs on the load transfer mechanism is evaluated. Pullout simulations considering different geometries of CCNTs are carried out to examine the tensile force and the interfacial shear stress (ISS). The results reveal that the maximal tensile force is reduced by increasing CCNT inner diameters, increasing the helix angles, and decreasing nanotube diameters. The distance between CCNTs and the polymer matrix is varied, and the interfacial distance favors greater ISS. Decreasing the inner diameter of the CCNT, the helix angle, and the tube diameter increases the ISS. The enhancement mechanism of CCNT/polymer composites has also been illustrated. Due to a lack of experimental results, only numerical results are given. The present study helps to understand the interfacial adhesion behavior between the polymer matrix and CCNTs and is expected to contribute to the development of CCNT reinforced polymer composites.
Antibiofilm Effect of Silver Nanoparticles in Changing the Biofilm-Related Gene Expression of Staphylococcus epidermidis
Authors: Swolana Denis , Kępa Małgorzata , Kruszniewska-Rajs Celina , Wojtyczka Robert D.
Published: August 2022

Nowadays, antibiotic resistance is a major public health problem. Among staphylococci, infections caused by Staphylococcus epidermidis (S. epidermidis) are frequent and difficult to eradicate. This is due to its ability to form biofilm. Among the antibiotic substances, nanosilver is of particular interest. Based on this information, we decided to investigate the effect of nanosilver on the viability, biofilm formation and gene expression of the icaADBC operon and the icaR gene for biofilm and non-biofilm S. epidermidis strains. As we observed, the viability of all the tested strains decreased with the use of nanosilver at a concentration of 5 µg/mL. The ability to form biofilm also decreased with the use of nanosilver at a concentration of 3 µg/mL. Genetic expression of the icaADBC operon and the icaR gene varied depending on the ability of the strain to form biofilm. Low concentrations of nanosilver may cause increased biofilm production, however no such effect was observed with high concentrations. This confirms that the use of nanoparticles at an appropriately high dose in any future therapy is of utmost importance. Data from our publication confirm the antibacterial and antibiotic properties of nanosilver. This effect was observed phenotypically and also by levels of gene expression.
MYB Transcription Factors Becoming Mainstream in Plant Roots
Authors: Chen Zhuo , Wu Zexuan , Dong Wenyu , Liu Shiying , Tian Lulu , Li Jiana , Du Hai
Published: August 2022

The function of the root system is crucial for plant survival, such as anchoring plants, absorbing nutrients and water from the soil, and adapting to stress. MYB transcription factors constitute one of the largest transcription factor families in plant genomes with structural and functional diversifications. Members of this superfamily in plant development and cell differentiation, specialized metabolism, and biotic and abiotic stress processes are widely recognized, but their roles in plant roots are still not well characterized. Recent advances in functional studies remind us that MYB genes may have potentially key roles in roots. In this review, the current knowledge about the functions of MYB genes in roots was summarized, including promoting cell differentiation, regulating cell division through cell cycle, response to biotic and abiotic stresses (e.g., drought, salt stress, nutrient stress, light, gravity, and fungi), and mediate phytohormone signals. MYB genes from the same subfamily tend to regulate similar biological processes in roots in redundant but precise ways. Given their increasing known functions and wide expression profiles in roots, MYB genes are proposed as key components of the gene regulatory networks associated with distinct biological processes in roots. Further functional studies of MYB genes will provide an important basis for root regulatory mechanisms, enabling a more inclusive green revolution and sustainable agriculture to face the constant changes in climate and environmental conditions.
Glucose–Thymidine Ratio as a Metabolism Index Using 18F-FDG and 18F-FLT PET Uptake as a Potential Imaging Biomarker for Evaluating Immune Checkpoint Inhibitor Therapy
Authors: Oh Sera , Youn Hyewon , Paeng Jin Chul , Kim Young-Hwa , Lee Chul-Hee , Choi Hongyoon , Kang Keon Wook , Chung June-Key , Cheon Gi Jeong
Published: August 2022

Immune checkpoint inhibitors (ICIs) are widely used in cancer immunotherapy, requiring effective methods for response monitoring. This study evaluated changes in 18F-2-fluoro-2-deoxy-D-glucose (FDG) and 18F-fluorothymidine (FLT) uptake by tumors following ICI treatment as potential imaging biomarkers in mice. Tumor uptakes of 18F-FDG and 18F-FLT were measured and compared between the ICI treatment and control groups. A combined imaging index of glucose–thymidine uptake ratio (GTR) was defined and compared between groups. In the ICI treatment group, tumor growth was effectively inhibited, and higher proportions of immune cells were observed. In the early phase, 18F-FDG uptake was higher in the treatment group, whereas 18F-FLT uptake was not different. There was no difference in 18F-FDG uptake between the two groups in the late phase. However, 18F-FLT uptake of the control group was markedly increased compared with the ICI treatment group. GTR was consistently higher in the ICI treatment group in the early and late phases. After ICI treatment, changes in tumor cell proliferation were observed with 18F-FLT, whereas 18F-FDG showed altered metabolism in both tumor and immune cells. A combination of 18F-FLT and 18F-FDG PET, such as GTR, is expected to serve as a potentially effective imaging biomarker for monitoring ICI treatment.
Unveiling the Ability of Witch Hazel (Hamamelis virginiana L.) Bark Extract to Impair Keratinocyte Inflammatory Cascade Typical of Atopic Eczema
Authors: Piazza Stefano , Martinelli Giulia , Magnavacca Andrea , Fumagalli Marco , Pozzoli Carola , Terno Massimo , Canilli Luisa , Angarano Marco , Maranta Nicole , Dell’Agli Mario , Sangiovanni Enrico
Published: August 2022

Hamamelis virginiana L. bark extract is a traditional remedy for skin affections, including atopic dermatitis/eczema (AD). Hamamelis preparations contain tannins, including hamamelitannin (HT), although their pharmacological role in AD is still unknown. This study aimed to study the rational for its topical use by considering the impact of crucial biomarkers on AD pathogenesis. A standardized extract (HVE) (0.5–125 μg/mL) was compared to hamamelitannin (HT), its main compound (0.5–5 μg/mL), in a model of human keratinocytes (HaCaTs), challenged with an AD-like cytokine milieu (TNF-α, IFN-γ, and IL-4). HVE inhibited the release of mediators involved in skin autoimmunity (IL-6 and IL-17C) and allergy (TSLP, IL-6, CCL26, and MMP-9) with a concentration-dependent fashion (IC50s < 25 μg/mL). The biological mechanism was ascribed, at least in part, to the impairment of the NF-κB-driven transcription. Moreover, HVE counteracted the proliferative effects of IL-4 and recovered K10, a marker of skin differentiation. Notably, HT showed activity on well-known targets of IL-4 pathway (CCL26, K10, cell proliferation). To the best of our knowledge, this work represents the first demonstration of the potential role of Hamamelis virginiana in the control of AD symptoms, such as itch and skin barrier impairment, supporting the relevance of the whole phytocomplex.
Landscape and Stand Characteristics Influence on the Bird Assemblage in Nothofagus antarctica Forests of Tierra del Fuego
Authors: Benitez Julieta , Barrera Marcelo D. , Rosas Yamina M. , Martínez Pastur Guillermo J. , Lencinas María V.
Published: August 2022

Different variables operate simultaneously at different spatial scales, influencing community composition and species distribution. This knowledge could improve management and conservation practices in managed menaced forests. The objective of this work was to determine the influence of landscape and stand variables on the bird assemblage of the managed Nothofagus antarctica forest of Tierra del Fuego (Argentina). We used data from bird point counts (three or four censuses during middle summer of two consecutive years) located at 48 sites distributed at four ranches. At each site, we extracted landscape variables with Fragstat software from the forest patches, the cover classes, and the whole landscape. We also evaluated local stand characteristics, such as forest structure, ground cover, and food availability, including understory plant cover usually consumed by birds and available arthropods. Data were evaluated by detrended and canonical correspondence analyses. We found that landscape configuration (e.g., forest patch shape) and local stand variables (e.g., canopy cover) influenced bird assemblage more than landscape composition. Moreover, bird functional groups responded differently to different spatial scale variables (e.g., forest specialist species were associated with forest structure, but species that use low strata to nest and feed were associated with landscape configuration variables), demonstrating the importance of using multiple spatial scales to better understand bird species requirements. The combination of practices that promote some local characteristics (e.g., high canopy cover) and more complex landscape configurations could simultaneously favor different bird species groups and improve the effectiveness of management and conservation strategies.
Assessing the Ability of Agricultural Socialized Services to Promote the Protection of Cultivated Land among Farmers
Authors: Cheng Changming , Gao Qiang , Qiu Yuqing
Published: August 2022

Protecting and improving the quality of cultivated land is important to agricultural modernization. Based on data from a survey of 706 rice-growing households in Hunan, Jiangxi and Jiangsu, this paper uses the Probit model, CMP method and Biprobit model to systematically analyze the impact of agricultural socialized services on farmers’ cultivated land quality protection behaviors. This study finds that agricultural socialized services have a significantly positive impact on the adoption of soil testing formulas and straw returning technology among farmers, which can improve both the probability and the degree of cultivated land quality protection. The heterogeneity test results suggest that agricultural socialized services provide a higher incentive for the older generation of farmers to adopt arable land quality protection. In addition, we found that agricultural socialized services are more conducive to the protection of contracted land than transferred land. Therefore, we recommend that policies on agricultural socialized services be further implemented, the supply of agricultural socialized services be optimized, and the role of service organizations in publicizing and promoting cultivated land protection techniques be brought into play. We also posit that the simultaneous encouragement of farmers in using agricultural socialized services would protect the quality of cultivated land. During this process, additional attention should be paid to the response differentiation of peasants with different characteristics.
Hybrid Density Functional Investigation of Cu Doping Impact on the Electronic Structures and Optical Characteristics of TiO2 for Improved Visible Light Absorption
Authors: Kanoun Mohammed Benali , Alshoaibi Adil , Goumri-Said Souraya
Published: August 2022

We report a theoretical investigation of the influence of Cu doping into TiO2 with various concentrations on crystal structure, stability, electronic structures and optical absorption coefficient using density functional theory via the hybrid formalism based on Heyd Scuseria Ernzerhof. Our findings show that oxygen-rich environments are better for fabricating Cu-doped materials and that the energy of formation for Cu doping at the Ti site is lower than for Cu doping at the O site under these environments. It is found that Cu doping introduces intermediate bands into TiO2, narrowing the band gap. Optical absorption curves show that the Cu-doped TiO2 can successfully harvest visible light. The presence of widely intermediate bands above the valence-band edge could explain the increase in the visible light absorption range. However, the intensity of visible light absorption rises with the increase in doping concentration.
The Combined Effect of Glass Fiber Mesh and Steel Fiber on Two-Layered Preplaced Aggregate Concrete against Drop Weight Impact
Authors: Karthikeyan Muniraj , Verapathran Maruthasalam , Abid Sallal R. , Murali Gunasekaran
Published: August 2022

Buildings and other infrastructure should be designed to withstand impact loads in terrorist attacks or industrial catastrophes. Fibrous concrete is utilized in a variety of ways in the construction of structures to resist impact loads. Preplaced aggregate fibrous concrete (PAFC) has a different production method than traditional fibrous concrete. Although PAFC offers several benefits over standard fibers in the construction of protective structures, there are relatively few investigations on the behavior of PAFC when exposed to impact loads. This research investigated the impact response of PFAC with the combined action of steel fibers and glass fiber mesh (GFM). Twenty-two mixtures were prepared and divided into two groups in which there were fibrous and non-fibrous specimens. The specimens from the first group comprised various diameters (ratio of reinforcement surface to total sample surface) of GFM and were provided in two and four layers. The second group of specimens is the same as the first group, including a 3% dosage of steel fibers. All specimens were subjected to a drop-weight impact test and the key parameters examined were cracking and failure impact energies, ductility index and failure pattern. The results indicated that the incorporation of GFM increased the impact performance and impact ductility, where the retained impact energies and the ductility index increased by increasing the ratio of reinforcement surface (diameter) of GFM and its number of layers. However, the positive influence of SF in enhancing the impact performance was way higher than that of GFM. The use of 3% hooked-end SF improved the failure impact energy by more than 3000%, while the maximum improvement gained by using four layers of 150 mm diameter (full reinforcement surface) GFM was approximately 400%.
AlSi10Mg in Powder Bed Fusion with Laser Beam: An Old and Boring Material?
Authors: Rasch Michael , Bartels Dominic , Sun Shoujin , Schmidt Michael
Published: August 2022

Powder bed fusion with laser beam of metals (PBF-LB/M) is a widely used technology to produce parts with a high freedom in design paired with excellent mechanical properties. The casting alloy AlSi10Mg features a wide process window and a microstructure with excellent mechanical properties which are not obtainable through conventional manufacturing. One possibility for achieving this is by influencing the solidification which then directly affects the local part properties. In this study, the effect of different laser beam profiles with gaussian and top-hat intensity distributions, as well as the influence of varying parameter sets on the microstructure and microhardness within the same specimen, was examined. A test specimen consisting of many small cubes was built with different parameters. It was found that the local properties can be varied in a wide range. Build-height-dependent in-situ aging effects can thereby be exploited for tailoring the local material properties. Thus, an extra degree of freedom is added to the design of additively manufactured parts.
Zeolites Reduce the Transfer of Potentially Toxic Elements from Soil to Leafy Vegetables
Authors: Cadar Oana , Stupar Zamfira , Senila Marin , Levei Levente , Moldovan Ana , Becze Anca , Ozunu Alexandru , Levei Erika Andrea
Published: August 2022

The ability of natural zeolite amendment to reduce the uptake of potentially toxic elements (PTEs) by lettuce, spinach and parsley was evaluated using pot experiments. PTE concentrations in roots and shoots, as well as the pseudo total (PT), water soluble (WS) and bioavailable (BA) PTE fractions in the amended soils, were assessed. Although the PT PTE concentration was high, the WS fraction was very low (<0.4%), while the BA fraction varied widely (<5% for Cr, Mn and Co, <15% for Ni, Pb and Zn, >20% for Cd and Cu). PTE concentration decreased in both roots and shoots of all leafy vegetables grown on zeolite amended soils, especially at high amendment dose (10%). The uptake of PTEs mainly depended on plant species, PTE type and amendment dose. With the exception of Zn in spinach, the bioaccumulation factor for roots was higher than for shoots. Generally, lettuce displayed the highest PTE bioaccumulation capacity, followed by spinach and parsley. Except for Zn in spinach, the transfer factors were below 1 for all PTEs, all plant species and all amendment doses. Our results showed that the natural zeolites are promising candidates in the reclamation of contaminated soils due to their ability to immobilize PTEs.
Reversible First-Order Single Crystal to Single Crystal Thermal Phase Transition in [(CH3)3CNH3]4[V4O12]
Authors: Vitoria Pablo , Wéry Ana , San Felices Leire , Bravo-García Laura , Ruiz-Bilbao Estibaliz , Laza José , Vilas José , Gutiérrez-Zorrilla Juan
Published: August 2022

The well-known compound tetrakis(tert-butylammonium)-cyclo-tetrametavanadate (V), [(CH3)3CNH3]4[V4O12] (1h_RT), which crystallizes in the tetragonal I4/m space group, undergoes an irreversible solid state transformation upon heating, constituting one of the few examples in which the initial and the final stages are structurally characterized by sc-XRD. Now, we observed the ability of the same compound to undergo an additional single-crystal-to-single-crystal (SCSC) transformation upon thermal stimuli, but this time at low temperatures (153 K). Compound 1h_RT contains a discrete unprotonated [V4O12]4− tetrahedral anion in which V and O bridging atoms are coplanar. In both phases, these tetrameric anions are linked through tert-butylammonium cations in an extensive network of hydrogen bonds, but at low temperatures, this phase loses its characteristic O-V-O coplanarity, with the resulting rearrangement of the crystal packing and hydrogen-bond network which provide its reversibility at low temperatures. Again, the initial and final stages have been characterized structurally by sc-XRD.
Some Moduli of Angles in Banach Spaces
Authors: Du Dandan , Ahmad Asif , Din Anwarud , Li Yongjin
Published: August 2022

In this paper, we mainly discuss the angle modulus of convexity δXa(ϵ) and the angle modulus of smoothness ρXa(ϵ) in a real normed linear space X, which are closely related to the classical modulus of convexity δX(ϵ) and the modulus of smoothness ρX(ϵ). Some geometric properties of the two moduli were investigated. In particular, we obtained a characterization of uniform non-squareness in terms of ρXa(1). Meanwhile, we studied the relationships between δXa(ϵ), ρXa(ϵ) and other geometric constants of real normed linear spaces through some equalities and inequalities. Moreover, these two coefficients were computed for some concrete spaces.
The Genus Iodidimonas: From Its Discovery to Potential Applications
Authors: Amachi Seigo , Iino Takao
Published: August 2022

The genus Iodidimonas was recently proposed in the class Alphaproteobacteria. Iodidimonas strains are aerobic, mesophilic, neutrophilic, moderately halophilic, and chemo-organotrophic. They were first discovered in natural gas brine water containing a very high level of iodide (I−). They exhibited a unique phenotypic feature of iodide oxidation to form molecular iodine (I2). Iodidimonas was also enriched and isolated from surface seawater supplemented with iodide, and it is clearer now that their common habitats are those enriched with iodide. In such environments, Iodidimonas species seem to attack microbial competitors with the toxic form I2 to occupy their ecological niche. The iodide-oxidizing enzyme (IOX) purified from the Iodidimonas sp. strain Q-1 exhibited high catalytic efficiency for iodide and consisted of at least two proteins IoxA and IoxC. IoxA is a putative multicopper oxidase with four conserved copper-binding regions but is phylogenetically distinct from other bacterial multicopper oxidases. The IOX/iodide system could be used as a novel enzyme-based antimicrobial system which can efficiently kill Bacillus spores. Furthermore, the IOX/iodide system can be applied to the decolorization of recalcitrant dyes, where iodide may function as a novel inorganic natural redox mediator.
Improved Cladocopium goreaui Genome Assembly Reveals Features of a Facultative Coral Symbiont and the Complex Evolutionary History of Dinoflagellate Genes
Authors: Chen Yibi , Shah Sarah , Dougan Katherine E. , van Oppen Madeleine J. H. , Bhattacharya Debashish , Chan Cheong Xin
Published: August 2022

Dinoflagellates of the family Symbiodiniaceae are crucial photosymbionts in corals and other marine organisms. Of these, Cladocopium goreaui is one of the most dominant symbiont species in the Indo-Pacific. Here, we present an improved genome assembly of C. goreaui combining new long-read sequence data with previously generated short-read data. Incorporating new full-length transcripts to guide gene prediction, the C. goreaui genome (1.2 Gb) exhibits a high extent of completeness (82.4% based on BUSCO protein recovery) and better resolution of repetitive sequence regions; 45,322 gene models were predicted, and 327 putative, topologically associated domains of the chromosomes were identified. Comparison with other Symbiodiniaceae genomes revealed a prevalence of repeats and duplicated genes in C. goreaui, and lineage-specific genes indicating functional innovation. Incorporating 2,841,408 protein sequences from 96 taxonomically diverse eukaryotes and representative prokaryotes in a phylogenomic approach, we assessed the evolutionary history of C. goreaui genes. Of the 5246 phylogenetic trees inferred from homologous protein sets containing two or more phyla, 35–36% have putatively originated via horizontal gene transfer (HGT), predominantly (19–23%) via an ancestral Archaeplastida lineage implicated in the endosymbiotic origin of plastids: 10–11% are of green algal origin, including genes encoding photosynthetic functions. Our results demonstrate the utility of long-read sequence data in resolving structural features of a dinoflagellate genome, and highlight how genetic transfer has shaped genome evolution of a facultative symbiont, and more broadly of dinoflagellates.
Autonomous Critical Help by a Robotic Assistant in the Field of Cultural Heritage: A New Challenge for Evolving Human-Robot Interaction
Authors: Cantucci Filippo , Falcone Rino
Published: August 2022

Over the years, the purpose of cultural heritage (CH) sites (e.g., museums) has focused on providing personalized services to different users, with the main goal of adapting those services to the visitors’ personal traits, goals, and interests. In this work, we propose a computational cognitive model that provides an artificial agent (e.g., robot, virtual assistant) with the capability to personalize a museum visit to the goals and interests of the user that intends to visit the museum by taking into account the goals and interests of the museum curators that have designed the exhibition. In particular, we introduce and analyze a special type of help (critical help) that leads to a substantial change in the user’s request, with the objective of taking into account the needs that the same user cannot or has not been able to assess. The computational model has been implemented by exploiting the multi-agent oriented programming (MAOP) framework JaCaMo, which integrates three different multi-agent programming levels. We provide the results of a pilot study that we conducted in order to test the potential of the computational model. The experiment was conducted with 26 real participants that have interacted with the humanoid robot Nao, widely used in Human-Robot interaction (HRI) scenarios.