bluuebunny/crossref_metadata_2025 huggingface dataset:
Chilean institutional policies and multi-level agents: Challenges from the COVID-19 pandemic and carbon footprint
Authors: Bergamini Kay , Ojeda Carolina G. , Gutiérrez Patricia , Salazar Gonzalo , Curillán Christian
Published: March 2023

Introduction

As a result of the increasing number of multilateral agreements that Chile has signed, different sectors of consumption have become sources of emissions. In this context attempts to implement guidelines to address this issue have been made. Nevertheless, international policies such as sustainable development goals (SDG) 11–12 often generate dissonance in national and local administrations and have been approached by different instruments to reduce the effects of emissions, mostly focused on the private industrial sector.

Methods

This article focuses on four of the most polluted cities in south-central Chile (Coronel, Temuco, Valdivia, and Osorno). Key agents (ministries, regional government, municipalities, and civil society) from three levels of policy development were selected at three scales (national, regional, and local) and interviewed considering three thematic axes: knowledge of carbon footprint areas (housing, heating, food, mobility, and energy), institutional governance, and adaptive changes due to COVID-19.

Results

The results show that in Chile, there is a multiscale climate governance led by the Ministry of the Environment (national level), followed by the regional and local levels. Citizens are then left with few capacities, which is negatively viewed. In relation to the carbon footprint and COVID-19, it can be observed that the topic of energy was more addressed at the national and regional levels. Food and energy, followed by heating and then mobility were addressed at the communal level and in civil society.

Discussion

Decision-making strategies and policies were discussed in this paper.
Influence of geomechanics parameters on stress sensitivity in fractured reservoir
Authors: Feng Yutian , Tang Hongming , Tang Haoxuan , Leng Yijiang , Shi Xuewen , Liu Jia , Wang Zhao , Deng Cong
Published: March 2023

The complex fractures aggravate stress sensitivity and heterogeneity of the reservoir and seriously restrict effective development. Therefore, it is of great significance to study and quantitatively evaluate the stress sensitivity of the fractured reservoir. Taking the typical block of the Longmaxi shale reservoir in southern Sichuan as the engineering background, one uses the finite element method to develop a numerical model of a two-dimensional fracture closure variation subjected to the non-hydrostatic stress field. It explores the influence of different fracture occurrences and rock mechanical parameters on stress sensitivity. The theoretical model verifies the numerical simulation results to reveal the stress sensitivity mechanism of the fractured reservoir. The results show that the influence of the dip angle of fracture on the stress sensitivity depends on the anisotropy of applied in-situ stresses. The stronger stress sensitivity occurs in low-dip angles where the lateral pressure coefficient is less than 1. One defines the lateral pressure coefficient. On the contrary, the stronger stress sensitivity occurs in high-dip angles where the lateral pressure coefficient is more significant than 1. It is because the normal stress differences under different stress fields apply to the fracture. Under a given stress condition, the stress sensitivity of fracture negatively correlates with aspect ratio, elastic modulus, and Poisson’s ratio. Pressure maintenance may be more critical in a reservoir with a low aspect ratio and rich in soft minerals. The theoretical predicting model of fracture permeability under different conditions is established based on the linear elastic theory. The relative error between theoretically predicted results and numerical simulation ones is less than 10%, which verifies the accuracy of numerical simulation results. The fundamental reason for stress sensitivity in the fractured reservoir is the fracture geometry and mineral deformation change. The research results are of great significance for establishing the productivity equation considering the stress sensitivity, accurately evaluating the variation of reservoir seepage capacity, and formulating reasonable drainage and production system.
Grades 7–12 teachers' perception of computational thinking for mathematics and technology
Authors: Humble Niklas , Mozelius Peter
Published: March 2023

Introduction

An ongoing trend on a global scale is the integration of computer science and programming in K-12 education. The integration has been motivated by the needs of the present and future labor market but also by the assumption that skills related to computer science and programming are valuable for citizens to navigate an increasingly digitalized society. Computational thinking (CT) is a concept that aims to define and summarize skills associated with programming and computer science and has received wide recognition within research and education. But how do the teachers perceive this concept, and how do they relate it to their own teaching and learning activities? This study aims to investigate and discuss teachers' perceptions of CT in grades 7–12 mathematics and technology.

Methods

Data have been collected from essay assignments in three instances of a professional development course on fundamental programming for grades 7–12 teachers in mathematics and technology. In the essays, the teachers reflect on CT in relation to mathematics and technology and teaching and learning activities in these subjects. With a theoretical framework for CT, the collected data have been analyzed with a directed content analysis approach to identify categories of interests for CT in relation to grades 7–12 mathematics and technology.

Results

The results of the study show that the teachers perceive both opportunities and challenges in applying the CT concept in their teaching and learning activities. For example, it can strengthen the subjects through new practices and reinforce old practices, but it could be too complex and perceived as difficult by some students. Furthermore, many of the teachers perceive CT not only to be relevant for mathematics and technology but also for learning in general.

Discussion

The conclusion of the study is that CT has the potential to enhance teaching and learning activities in mathematics, technology, and other STEM subjects. If this should be successful, CT must not be involved too abstractly or too superficially. This study contributes to the discussion on CT in K-12 education, adding the teachers' perspective. The findings of this study can be used by teachers and other stakeholders in the design of classroom activities that apply the CT concept.
Relationship between bone mineral density and hyperuricemia in obesity: A cross-sectional study
Authors: Zhang Yi , Tan Min , Liu Boyu , Zeng Manxia , Zhou You , Zhang Mengru , Wang Yikai , Wu Jing , Wang Min
Published: March 2023

Background

Obesity is an increasingly severe global public health issue. This study aims to estimate the cross-sectional association between bone mineral density (BMD) and hyperuricemia (HU) in obesity.

Method

A total of 275 obese subjects (126 men and 149 women) participated in this cross-sectional study. Obesity was diagnosed as body mass index (BMI) ≥28 kg/m2, whereas HU was defined as the blood uric acid level of 416 μmol/L in men and 360 μmol/L in women. The BMD of the lumbar spine and right hip was measured by dual-energy X-ray absorptiometry (DXA). The multivariable logistic regressions were employed to examine the relationship between BMD and HU in obesity, with the adjustment of gender, age, fasting blood glucose, fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR), cholesterol, triglycerides, low-density lipoprotein, high-density lipoprotein, creatinine, blood urea nitrogen, high-sensitivity C-reactive protein (hs-CRP), cigarette smoking, and alcohol drinking status.

Result

The overall prevalence of HU was 66.9% in this obese population. The mean age and BMI of this population were 27.9 ± 9.9 years and 35.2 ± 5.2 kg/m2, respectively. The multivariable-adjusted OR (the highest vs. lowest BMD quartile) demonstrated a negative relationship between BMD and HU in total (OR = 0.415, 95%CI: 0.182–0.946; p = 0.036), L1 (OR = 0.305, 95%CI: 0.127–0.730; p = 0.008), L2 (OR = 0.405, 95%CI: 0.177–0.925; p = 0.032), and L3 (OR = 0.368, 95%CI: 0.159–0.851; p = 0.020) lumbar vertebrae. In the subgroup analysis for the male population, the BMD was also negatively associated with HU in total (OR = 0.077, 95%CI: 0.014–0.427; p = 0.003), L1 (OR = 0.019, 95%CI: 0.002–0.206; p = 0.001), L2 (OR = 0.161, 95%CI: 0.034–0.767; p = 0.022), L3 (OR = 0.186, 95%CI: 0.041–0.858; p = 0.031), and L4 (OR = 0.231, 95%CI: 0.056–0.948; p = 0.042) lumbar vertebrae. However, such findings did not exist in women. In addition, there was no significant relationship between hip BMD and HU in obesity.

Conclusion

Our results showed that the lumbar BMD was negatively associated with HU in obesity. However, such findings only existed in men, rather than women. In addition, no significant relationship between hip BMD and HU existed in obesity. Due to the limited sample size and nature of the cross-sectional design, further large prospective studies are still needed to clarify the issues.
Urinary extracellular vesicles carry valuable hints through mRNA for the understanding of endocrine hypertension
Authors: Friso Simonetta , Castagna Annalisa , Mango Gabriele , Olivieri Oliviero , Pizzolo Francesca
Published: March 2023

Urinary extracellular vesicles (uEVs), released from cells of the urogenital tract organs, carry precious information about originating tissues. The study of molecules transported through uEVs such as proteins, lipids and nucleic acids provides a deeper understanding of the function of the kidney, an organ involved in the pathogenesis of hypertension and a target of hypertension-mediated organ damage. Molecules derived from uEVs are often proposed for the study of disease pathophysiology or as possible disease diagnostic and prognostic biomarkers. Analysis of mRNA loading within uEVs may be a unique and readily obtainable way to assess gene expression patterns of renal cells, otherwise achievable only by an invasive biopsy procedure. Interestingly, the only few studies investigating transcriptomics of hypertension-related genes through the analysis of mRNA from uEVs are inherent to mineralocorticoid hypertension. More specifically, it has been observed that perturbation in human endocrine signalling through mineralcorticoid receptors (MR) activation parallels changes of mRNA transcripts in urine supernatant. Furthermore, an increased copy number of uEVs-extracted mRNA transcripts of the 11β-hydroxysteroid dehydrogenase type 2 (HSD11B2) gene were detected among subjects affected by apparent mineralocorticoid excess (AME), a hypertension-inducing autosomal recessive disorder due to a defective enzyme function. Moreover, by studying uEVs mRNA, it was observed that the renal sodium chloride cotransporter (NCC) gene expression is modulated under different conditions related to hypertension. Following this perspective, we illustrate here the state of the art and the possible future of uEVs transcriptomics towards a deeper knowledge of hypertension pathophysiology and ultimately more tailored investigational, diagnostic-prognostic approaches.
Non-coding regions of nuclear-DNA-encoded mitochondrial genes and intergenic sequences are targeted by autoantibodies in breast cancer
Authors: Obaidat Deya , Giordo Roberta , Kleinbrink Erica L. , Banisad Emilia , Grossman Lawrence I. , Arshad Rooshan , Stark Azadeh , Maroun Marie-Claire , Lipovich Leonard , Fernandez-Madrid Félix
Published: March 2023

Autoantibodies against mitochondrial-derived antigens play a key role in chronic tissue inflammation in autoimmune disorders and cancers. Here, we identify autoreactive nuclear genomic DNA (nDNA)-encoded mitochondrial gene products (GAPDH, PKM2, GSTP1, SPATA5, MFF, TSPOAP1, PHB2, COA4, and HAGH) recognized by breast cancer (BC) patients’ sera as nonself, supporting a direct relationship of mitochondrial autoimmunity to breast carcinogenesis. Autoreactivity of multiple nDNA-encoded mitochondrial gene products was mapped to protein-coding regions, 3’ untranslated regions (UTRs), as well as introns. In addition, autoantibodies in BC sera targeted intergenic sequences that may be parts of long non-coding RNA (lncRNA) genes, including LINC02381 and other putative lncRNA neighbors of the protein-coding genes ERCC4, CXCL13, SOX3, PCDH1, EDDM3B, and GRB2. Increasing evidence indicates that lncRNAs play a key role in carcinogenesis. Consistent with this, our findings suggest that lncRNAs, as well as mRNAs of nDNA-encoded mitochondrial genes, mechanistically contribute to BC progression. This work supports a new paradigm of breast carcinogenesis based on a globally dysfunctional genome with altered function of multiple mitochondrial and non-mitochondrial oncogenic pathways caused by the effects of autoreactivity-induced dysregulation of multiple genes and their products. This autoimmunity-based model of carcinogenesis will open novel avenues for BC treatment.
Regulation of ncRNAs involved with ferroptosis in various cancers
Authors: Hu Chenxi , Zeng Xiangbo , Zhu Yuanchao , Huang Zehai , Liu Jiacheng , Ji Ding , Zheng Zaosong , Wang Qiong , Tan Wanlong
Published: March 2023

As a special pattern of programmed cell death, ferroptosis is reported to participate in several processes of tumor progression, including regulating proliferation, suppressing apoptotic pathways, increasing metastasis, and acquiring drug resistance. The marked features of ferroptosis are an abnormal intracellular iron metabolism and lipid peroxidation that are pluralistically modulated by ferroptosis-related molecules and signals, such as iron metabolism, lipid peroxidation, system Xc−, GPX4, ROS production, and Nrf2 signals. Non-coding RNAs (ncRNAs) are a type of functional RNA molecules that are not translated into a protein. Increasing studies demonstrate that ncRNAs have a diversity of regulatory roles in ferroptosis, thus influencing the progression of cancers. In this study, we review the fundamental mechanisms and regulation network of ncRNAs on ferroptosis in various tumors, aiming to provide a systematic understanding of recently emerging non-coding RNAs and ferroptosis.
The regulatory effects of p53 on the typical and atypical ferroptosis in the pathogenesis of osteosarcoma: A systematic review
Authors: Wang Linfeng , Pan Su
Published: March 2023

Study background: As a rare condition, osteosarcoma affects approximately 3% of all cancer patients. Its exact pathogenesis remains largely unclear. The role of p53 in up- and down-regulating atypical and typical ferroptosis in osteosarcoma remains unclear. The primary objective of the present study is investigating the role of p53 in regulating typical and atypical ferroptosis in osteosarcoma.

Methods: The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) and the Patient, Intervention, Comparison, Outcome, and Studies (PICOS) protocol were used in the initial search. The literature search was performed in six electronic databases, including EMBASE, Cochrane library of trials, Web of Science, PubMed, Google Scholar, and Scopus Review, using keywords connected by Boolean operators. We focused on studies that adequately defined patient profiles described by PICOS.

Results and discussion: We found that p53 played fundamental up- and down-regulatory roles in typical and atypical ferroptosis, resulting in either advancement or suppression of tumorigenesis, respectively. Direct and indirect activation or inactivation of p53 downregulated its regulatory roles in ferroptosis in osteosarcoma. Enhanced tumorigenesis was attributed to the expression of genes associated with osteosarcoma development. Modulation of target genes and protein interactions, especially SLC7A11, resulted in enhanced tumorigenesis.

Conclusion: Typical and atypical ferroptosis in osteosarcoma were regulatory functions of p53. The activation of MDM2 inactivated p53, leading to the downregulation of atypical ferroptosis, whereas activation of p53 upregulated typical ferroptosis. Further studies should be performed on the regulatory roles of p53 to unmask its possible clinical applications in the management of osteosarcoma.
ACP-GBDT: An improved anticancer peptide identification method with gradient boosting decision tree
Authors: Li Yanjuan , Ma Di , Chen Dong , Chen Yu
Published: March 2023

Cancer is one of the most dangerous diseases in the world, killing millions of people every year. Drugs composed of anticancer peptides have been used to treat cancer with low side effects in recent years. Therefore, identifying anticancer peptides has become a focus of research. In this study, an improved anticancer peptide predictor named ACP-GBDT, based on gradient boosting decision tree (GBDT) and sequence information, is proposed. To encode the peptide sequences included in the anticancer peptide dataset, ACP-GBDT uses a merged-feature composed of AAIndex and SVMProt-188D. A GBDT is adopted to train the prediction model in ACP-GBDT. Independent testing and ten-fold cross-validation show that ACP-GBDT can effectively distinguish anticancer peptides from non-anticancer ones. The comparison results of the benchmark dataset show that ACP-GBDT is simpler and more effective than other existing anticancer peptide prediction methods.
Utilization of a novel 3D radiologic scoring method to define therapeutic response and surgical candidacy
Authors: Rahmanuddin Syed , Von Hoff Daniel D. , Chaudhry Ammar , Guidaben Danielle , Khan Marjaan , Boswell William , Cridebring Derek , Brase Jordyn , Fong Yuman , Motarjem Pejman , Borazanci Erkut
Published: March 2023

Purpose

Neoadjuvant therapy and surgical resection can improve the survival rate of patients who receive a diagnosis of pancreatic cancer and shows to be potentially curative. The aim of this study is to define a novel CT perfusion analytical method by observing the treatment response of pancreatic cancer patients in a neoadjuvant-treated population to determine surgical candidacy.

Experimental design

This prospective study involved 22 adult patients with pancreatic ductal adenocarcinoma (PDAC). Participants received neoadjuvant therapy (paricalcitol, paclitaxel protein-bound, cisplatin, and gemcitabine) for up to 6 months. The study examined differences in density between the arterial and venous phases of CT scans using a mathematical analysis called the Marley equation. The data was used to assess treatment responses and determined whether a patient can become a surgical candidate. The consideration for surgical candidacy was defined by Dr. Rahmanuddin, termed the “R” score and graphically depicted as the “R” Clock. The R score determined the number of tumor-linked blood vessels. Any vessel associated with tumor involvement received a score of 1. Patients who received a score of 5 or less were eligible for surgery. 3D Tumor volumetric analyses were performed using GE AW 3D software to assess the treatment response associated with tumor perfusion.

Results

Visual differences in vascular involvement between baseline and final imaging were associated with a higher likelihood of proceeding to surgery. After administration of the neoadjuvant therapy, 81% of patients (18 of 22) received an R score of 5 or less, deeming all of them eligible for surgery. A total of 59% of patients (13 of 22) proceeded with the surgery. Changes in arterial and venous perfusion reflected tumor aggressiveness as defined by the Marley equation.

Conclusion

CT vessel perfusion using the R score and Marley Equation might be helpful in defining the surgical candidacy of PDAC patients when used in conjunction with 3D tumor volumetric quantification. The parameters defined by the R score determined higher perfusion scores as having greater vascular growth, and patients with tumor involvement of more than six vessels were deemed surgically unresectable. The Marley equation demonstrated tumor aggression via changes in arterial and venous perfusion. Additional studies are needed to further validate these methodologies and assess their clinical utility.
Identification of immunodiagnostic blood biomarkers associated with spinal cord injury severity
Authors: Li Jianfeng , Liu Xizhe , Wang Jianmin , Wang Fuan , Zhu Zhengya , Tang Tao , Wang Jun , Zhou Zhiyu , Gao Manman , Liu Shaoyu
Published: March 2023

Blood always shows some immune changes after spinal cord injury (SCI), and detection of such changes in blood may be helpful for diagnosis and treatment of SCI. However, studies to date on blood immune changes after SCI in humans are not comprehensive. Therefore, to obtain the characteristics of blood immune changes and immunodiagnostic blood biomarkers of SCI and its different grades, a human blood transcriptome sequencing dataset was downloaded and analyzed to obtain differentially expressed immune-related genes (DEIGs), related functions and signaling pathways related to SCI and its various grades. Characteristic biomarkers of SCI and its different grades were identified by using weighted gene coexpression network analysis (WGCNA) and least absolute shrinkage and selection operator (LASSO) logistic regression. Expression of biomarkers was verified through experiments. The area under the curve (AUC) of biomarkers was calculated to evaluate their diagnostic value, and differences in immune cell content were examined. In this study, 17 kinds of immune cells with different contents between the SCI group and healthy control (HC) group were identified, with 7 immune cell types being significantly increased. Differences in the content of immune cells between different grades of SCI and the HC group were also discovered. DEIGs were identified, with alteration in some immune-related signaling pathways, vascular endothelial growth factor signaling pathways, and axon guidance signaling pathways. The SCI biomarkers identified and those of American Spinal Injury Society Impairment Scale (AIS) A and AIS D of SCI have certain diagnostic sensitivity. Analysis of the correlation of immune cells and biomarkers showed that biomarkers of SCI, AIS A grade and AIS D grade correlated positively or negatively with some immune cells. CKLF, EDNRB, FCER1G, SORT1, and TNFSF13B can be used as immune biomarkers for SCI. Additionally, GDF11and HSPA1L can be used as biomarkers of SCI AIS A grade; PRKCA and CMTM2 can be used as biomarkers of the SCI AIS D grade. Detecting expression of these putative biomarkers and changes in related immune cells may be helpful for predicting the severity of SCI.
Progress in the clinical application of immune checkpoint inhibitors in small cell lung cancer
Authors: He Jiahui , Hu Qinyong
Published: March 2023

Small cell lung cancer (SCLC) is a refractory cancer with poor prognosis due to its aggressive malignancy and high rates of metastasis, recurrence and drug resistance. These characteristics have also greatly impeded the identification of new treatment methods and drugs. The traditional model of SCLC treatment that has been reliant on platinum combined with etoposide for decades has been superseded by the emergence of immune checkpoint inhibitors (ICIs), which have shown significant therapeutic effects and broad application prospects as a monotherapy. This has led to the evaluation of ICIs with different mechanisms of action and their use in combination with radiotherapy or a variety of molecular targeted drugs to achieve synergy, complementary advantages, and reduce adverse reactions. Here, we review the progress in the use of ICIs as a monotherapy or in combination therapy for SCLC and consider the current limitations of these approaches as well as prospects for future developments.
Exosomes, MDSCs and Tregs: A new frontier for GVHD prevention and treatment
Authors: Hess Nicholas J. , Kink John A. , Hematti Peiman
Published: March 2023

The development of graft versus host disease (GVHD) represents a long-standing complication of allogeneic hematopoietic cell transplantation (allo-HCT). Different approaches have been used to control the development of GVHD with most relying on variations of chemotherapy drugs to eliminate allo-reactive T cells. While these approaches have proven effective, it is generally accepted that safer, and less toxic GVHD prophylaxis drugs are required to reduce the health burden placed on allo-HCT recipients. In this review, we will summarize the emerging concepts revolving around three biologic-based therapies for GVHD using T regulatory cells (Tregs), myeloid-derived-suppressor-cells (MDSCs) and mesenchymal stromal cell (MSC) exosomes. This review will highlight how each specific modality is unique in its mechanism of action, but also share a common theme in their ability to preferentially activate and expand Treg populations in vivo. As these three GVHD prevention/treatment modalities continue their path toward clinical application, it is imperative the field understand both the biological advantages and disadvantages of each approach.
Runoff from upstream changes the structure and energy flow of food web in estuary
Authors: Yi Yujun , Zhao Fanxuan , Liu Qi , Song Jie
Published: March 2023

Introduction

Sediment and nutrients flow into estuary with runoff, and then influence the estuary ecosystem. Much work has been done for investigating the response of water quality and species group (eg. phytoplankton or zooplankton) to the runoff from upstream, while few research has been taken to evaluate the response of the whole ecosystem.

Methods

Food webs of different seasons and regions were established based on stable isotope analysis and Bayesian mixing model. The influences of upstream runoff and sediment transport on the estuarine food webs were analyzed.

Results

Food web in estuary had highly spatial-temporal variability. The stable isotope values of organisms were higher on the northern shore than that on the southern shore. The area with high-turbidity freshwater inflow nurtured more terrestrial- organic- matter (TOM) relying species. And the contribution of TOM to food web was higher in flood season than that in non-flood season. The trophic levels of major consumers in the non-flood season were generally higher than that in the flood season. Significant differences in the average TP of species between two shores appeared during the non-flood season (P < 0.05). Expect for the C value, all of the topological indexes of food webs on the northern shore were higher than those on the southern shore, and they were higher in the flood period.

Discussion

The input and diffusion of sediment and nutrients carried by runoff led to the changes in the dietary structure of estuarine organisms and the decrease in trophic levels of major consumers. At the same time, flow pulse with high sediment also aggravated the spatial differences of the structure of food webs. The higher contribution of TOM to consumers increased the link density of food web on the southern shore, making it a more robust system. However, the high diversity of food sources and aquatic species made the food web more complex on the northern shore.
Trace element composition of modern planktic foraminifera from an oxygen minimum zone: Potential proxies for an enigmatic environment
Authors: Davis Catherine V. , Doherty Shannon , Fehrenbacher Jennifer , Wishner Karen
Published: March 2023

Oxygen limited marine environments, such as oxygen minimum zones, are of profound importance for global nutrient cycling and vertical habitat availability. While it is understood that the extent and intensity of oxygen minimum zones are responsive to climate, the limited suite of viable proxies for low oxygen pelagic environments continues to pose a real barrier for paleoclimate interpretations. Here we investigate the proxy potential of an array of trace element (Mg, Mn, Zn, and Sr) to Ca ratios from the shells of Globorotaloides hexagonus, a planktic foraminifer endemic to tropical through temperate oxygen minimum zones. A species-specific relationship between Mg/Ca and temperature is proposed for quantitative reconstruction of oxygen minimum zone paleotemperatures. Both Mn/Ca and Zn/Ca ratios vary with oxygen concentration and could be useful for reconstructing G. hexagonus habitat where the primary signal can be d\istinguished from diagenetic overprinting. Finally, a robust correlation between Sr/Ca ratios and dissolved oxygen demonstrates a role for Sr as an indicator of oxygen minimum zone intensity, potentially via foraminiferal growth rate. The analysis of these relatively conventional trace element ratios in the shells of an oxygen minimum zone species has tremendous potential to facilitate multiproxy reconstructions from this enigmatic environment.
Polarized laser scattering detection of subsurface damage of quartz glass induced by grinding
Authors: Shi Fangyuan , Qiao Guowen , Lv Qixin , Bai Qian
Published: March 2023

Subsurface damage (SSD) is inevitably generated during the grinding process of quartz glass. It has a great impact on sustainability, lifetime and optical performance of quartz glass components and systems. This paper proposes a non-destructive detection method for detecting SSD in ground quartz glass based on polarized laser scattering (PLS). A PLS system was built to detect SSD in ground quartz glass samples prepared with different abrasive particle sizes. The PLS detection signal value had a positive correlation with the SSD depth. The finite-difference time-domain (FDTD) method was used to simulate the laser scattering process at the SSD. The distribution of electric field intensity could reflect the location of SSD. It is concluded that the PLS system can effectively detect SSD in the ground quartz glass.
Prior treated tuberculosis and mortality risk in lung cancer
Authors: Liao Kuang-Ming , Lee Chung-Shu , Wu Yu-Cih , Shu Chin-Chung , Ho Chung-Han
Published: March 2023

Background

Lung cancer is one of the leading causes of cancer death worldwide, and tuberculosis (TB) is a common pre-existing disease. However, there is scarce literature studying the mortality risk in patients with prior TB and subsequent lung cancer.

Methods

We recruited lung cancer patients from the Taiwan Cancer Registry from 2011 to 2015 and classified them into two groups according to presence or absence of prior TB. We then matched them in a ratio of 1:4 using the exact matching approach. The mortality risk within 3 years after diagnosis of lung cancer was analyzed and compared between these two groups.

Results

During the study period, 43,472 patients with lung cancer were recruited, and of these, 1,211 (2.79%) patients had prior TB. After matching, this cohort included 5,935 patients with lung cancer in two groups: patients with prior TB before lung cancer (n = 1,187) and those without (n = 4,748). After controlling for demographic factors and comorbidities, the patients with prior TB had increased adjusted hazard ratios of 1.13 (95% CI: 1.04–1.23) and 1.11 (1.02–1.21) for all-cause and cancer-specific 3-year mortality, respectively, compared to the lung cancer patients without prior TB. Duration between TB and lung cancer (<1 year vs. 1–3 years vs. >3 years) had no differences for mortality risk.

Conclusion

In the present study, 2.79% patients with lung cancer had prior TB, which was associated with higher 3-year mortality after they developed lung cancer. The mortality risk with prior TB did not decrease even if >3 years passed before diagnosis of lung cancer.
Design strategy and mechanism of nitrite oxidation suppression of elevated loading rate partial nitritation system
Authors: Ikem Juliet , Chen Huiyu , Delatolla Robert
Published: March 2023

There is a current need for a low operational intensity, effective and small footprint system to achieve stable partial nitritation for subsequent anammox treatment at mainstream municipal wastewaters. This research identifies a unique design strategy using an elevated total ammonia nitrogen (TAN) surface area loading rate (SALR) of 5 g TAN/m2.d to achieve cost-effective, stable, and elevated rates of partial nitritation in a moving bed biofilm reactor (MBBR) system under mainstream conditions. The elevated loaded partial nitritation MBBR system achieves a TAN surface area removal rate (SARR) of 2.01 ± 0.07 g TAN/m2.d and NO2−-N: NH4+-N stoichiometric ratio of 1.15:1, which is appropriate for downstream anammox treatment. The elevated TAN SALR design strategy promotes nitrite-oxidizing bacteria (NOB) activity suppression rather than a reduction in NOB population as the reason for the suppression of nitrite oxidation in the mainstream elevated loaded partial nitritation MBBR system. NOB activity is limited at an elevated TAN SALR likely due to thick biofilm embedding the NOB population and competition for dissolved oxygen (DO) with ammonia-oxidizing bacteria for TAN oxidation to nitrite within the biofilm structure, which ultimately limits the uptake of DO by NOB in the system. Therefore, this design strategy offers a cost-effective and efficient alternative for mainstream partial nitritation MBBR systems at water resource recovery facilities.
The effect of intensive statin therapy in non-symptomatic intracranial arteries: The STAMINA-MRI sub-study
Authors: Sim Jae Eun , Song Ha-Na , Choi Jong-Un , Lee Ji-Eun , Baek In Young , Cho Hwan-Ho , Kim Jong-Hoon , Chung Jong-Won , Kim Gyeong-Moon , Park Hyun-Jin , Bang Oh Young , Seo Woo-Keun
Published: March 2023

Background and aims

Pleiotropic effects of statins result in the stabilization of symptomatic intracranial arterial plaque. However, little is known about the effect of statins in non-symptomatic cerebral arteries. We hypothesized that intensive statin therapy could produce a change in the non-symptomatic cerebral arteries.

Methods

This is a sub-study of a prospective observational study under the title of “Intensive Statin Treatment in Acute Ischemic Stroke Patients with Intracranial Atherosclerosis: a High-Resolution Magnetic Resonance Imaging (HR-MRI) study.” Patients with statin-naive acute ischemic stroke who had symptomatic intracranial artery stenosis (above 50%) were recruited for this study. HR-MRI was performed to assess the patients’ cerebral arterial status before and 6 months after the statin therapy. To demonstrate the effect of statins in the non-symptomatic segment of intracranial cerebral arteries, we excluded symptomatic segments from the data to be analyzed. We compared the morphological changes using cerebrovascular morphometry.

Results

A total of 54 patients (mean age: 62.9 ± 14.4 years, 59.3% women) were included in this study. Intensive statin therapy produced significant morphological changes of overall cerebral arteries. Among the morphological features, the arterial luminal area showed the highest number of significant changes with a range from 5.7 and 6.7%. Systolic blood pressure (SBP) was an independent factor associated with relative changes in posterior circulation bed maximal diameter percentage change (beta −0.21, 95% confidence interval −0.36 to −0.07, p = 0.005).

Conclusion

Intensive statin therapy produced a favorable morphological change in cerebral arteries of not only the target arterial segment but also non-symptomatic arterial segments. The change in cerebral arterial luminal diameter was influenced by the baseline SBP and was dependent on the topographic distribution of the cerebral arteries.

Clinical Trial Registration: ClinicalTrials.gov, identifier NCT02458755.
Effects of repetitive practice of motor tasks on somatosensory gating
Authors: Akaiwa Mayu , Matsuda Yuya , Saito Hidekazu , Shibata Eriko , Sasaki Takeshi , Sugawara Kazuhiro
Published: March 2023

Introduction

During voluntary muscle contraction, the amplitude of the somatosensory evoked potential (SEP) is reduced by inhibiting sensory information from a peripheral nerve supplying the contracted muscle. This phenomenon is called “gating.” We reported that participants with good motor skills indicated strong suppression of somatosensory information. The present study investigated the effects of motor performance improvement following repetitive practice on the SEP amplitude.

Methods

The ball rotation task (BR task) was practiced by 15 healthy participants repetitively. SEPs were recorded before (pre) and after (post) repetitive practice.

Results

The BR task performance was significantly improved and the required muscle activation to perform the task was significantly reduced after the repetitive practice. The degree of gating was not significant between pre and post- for the SEP amplitude. A significant correlation was found between changes in SEP amplitude from pre to post and performance improvement.

Discussion

After repetitive practice, the degree of gating did not change, but the performance of the BR task improved, and the muscle activity required for the BR task decreased. These results suggest that repetitive practice does not change the degree of gating but changes the mechanism of gating. Furthermore, they indicate that suppression of the somatosensory area may play a role in improving task performance.
Predictability alters multisensory responses by modulating unisensory inputs
Authors: Smyre Scott A. , Bean Naomi L. , Stein Barry E. , Rowland Benjamin A.
Published: March 2023

The multisensory (deep) layers of the superior colliculus (SC) play an important role in detecting, localizing, and guiding orientation responses to salient events in the environment. Essential to this role is the ability of SC neurons to enhance their responses to events detected by more than one sensory modality and to become desensitized (‘attenuated’ or ‘habituated’) or sensitized (‘potentiated’) to events that are predictable via modulatory dynamics. To identify the nature of these modulatory dynamics, we examined how the repetition of different sensory stimuli affected the unisensory and multisensory responses of neurons in the cat SC. Neurons were presented with 2HZ stimulus trains of three identical visual, auditory, or combined visual–auditory stimuli, followed by a fourth stimulus that was either the same or different (‘switch’). Modulatory dynamics proved to be sensory-specific: they did not transfer when the stimulus switched to another modality. However, they did transfer when switching from the visual–auditory stimulus train to either of its modality-specific component stimuli and vice versa. These observations suggest that predictions, in the form of modulatory dynamics induced by stimulus repetition, are independently sourced from and applied to the modality-specific inputs to the multisensory neuron. This falsifies several plausible mechanisms for these modulatory dynamics: they neither produce general changes in the neuron’s transform, nor are they dependent on the neuron’s output.
Gut microbiome-modulated dietary strategies in EAE and multiple sclerosis
Authors: Hoffman Kristina , Doyle William J. , Schumacher Sean M. , Ochoa-Repáraz Javier
Published: March 2023

Over the last few decades, the incidence of multiple sclerosis has increased as society’s dietary habits have switched from a whole foods approach to a high fat, high salt, low dietary fiber, and processed food diet, termed the “Western diet.” Environmental factors, such as diet, could play a role in the pathogenesis of multiple sclerosis due to gut microbiota alterations, gut barrier leakage, and subsequent intestinal inflammation that could lead to exacerbated neuroinflammation. This mini-review explores the gut microbiome alterations of various dietary strategies that improve upon the “Western diet” as promising alternatives and targets to current multiple sclerosis treatments. We also provide evidence that gut microbiome modulation through diet can improve or exacerbate clinical symptoms of multiple sclerosis, highlighting the importance of including gut microbiome analyses in future studies of diet and disease.
Somatic and germline aberrations in homologous recombination repair genes in Chinese prostate cancer patients
Authors: Liu Yixiao , Jin Bo , Shen Cheng , Gao Xianshu , Qi Xin , Ma Mingwei , Li Hongzhen , Hao Han , Tang Qi , Yang Kaiwei , Mi Yue , Guan Jie , Feng Xuero , He Zhisong , Li Haixia , Yu Wei
Published: March 2023

Simple summary

Somatic and germline aberrations in homologous recombinant repair (HHR) genes are associated with increased incidence and poor prognosis for prostate cancer. Through next-generation sequencing of prostate cancer patients across all clinical states from north China, here the authors identified a somatic mutational rate of 3% and a germline mutational rate of 3.9% for HRR genes using 200 tumor tissues and 714 blood specimens. Thus, mutational rates in HRR genes were lower compared with previous studies.

Background

Homologous recombination repair deficiency is associated with higher risk and poorer prognosis for prostate cancer. However, the landscapes of somatic and germline mutations in these genes remain poorly defined in Chinese patients, especially for those with localized disease and those from north part of China. In this study, we explore the genomic profiles of these patients.

Methods

We performed next-generation sequencing with 200 tumor tissues and 714 blood samples from prostate cancer patients at Peking University First Hospital, using a 32 gene panel including 19 homologous recombination repair genes.

Results

TP53, PTEN, KRAS were the most common somatic aberrations; BRCA2, NBN, ATM were the most common germline aberrations. In terms of HRR genes, 3% (6/200) patients harbored somatic aberrations, and 3.8% (28/714) patients harbored germline aberrations. 98.0% (196/200) somatic-tested and 72.7% (519/714) germline tested patients underwent prostatectomy, of which 28.6% and 42.0% had Gleason scores ≥8 respectively. Gleason scores at either biopsy or prostatectomy were predictive for somatic aberrations in general and in TP53; while age of onset <60 years old, PSA at diagnosis, and Gleason scores at biopsy were clinical factors associated with positive germline aberrations in BRCA2/ATM.

Conclusions

Our results showed a distinct genomic profile in homologous recombination repair genes for patients with prostate cancer across all clinical states from north China. Clinicians may consider to expand the prostate cancer patients receiving genetic tests to include more individuals due to the weak guiding role by the clinical factors currently available.
Advances in molecular and cell therapy for immunotherapy of cholangiocarcinoma
Authors: Zhao Li-ming , Shi An-da , Yang Yan , Liu Zeng-li , Hu Xiao-Qiang , Shu Li-Zhuang , Tang Yong-chang , Zhang Zong-li
Published: March 2023

Cholangiocarcinoma (CCA) is a highly malignant tumor of the hepatobiliary system that has failed to respond to many traditional therapies to a certain extent, including surgery, chemotherapy and radiotherapy. In recent years, the new therapeutic schemes based on immunology have fundamentally changed the systemic treatment of various malignant tumors to a certain extent. In view of the immunogenicity of CCA, during the occurrence and development of CCA, some immunosuppressive substances are released from cells and immunosuppressive microenvironment is formed to promote the escape immune response of its own cells, thus enhancing the malignancy of the tumor and reducing the sensitivity of the tumor to drugs. Some immunotherapy regimens for cholangiocarcinoma have produced good clinical effects. Immunotherapy has more precise characteristics and less adverse reactions compared with traditional treatment approaches. However, due to the unique immune characteristics of CCA, some patients with CCA may not benefit in the long term or not benefit at all after current immunotherapy. At present, the immunotherapy of CCA that have been clinically studied mainly include molecular therapy and cell therapy. In this article, we generalized and summarized the current status of immunotherapy strategies including molecular therapy and cell therapy in CCA in clinical studies, and we outlined our understanding of how to enhance the clinical application of these immunotherapy strategies.
Near-infrared fluorescence imaging of hepatocellular carcinoma cells regulated by β-catenin signaling pathway
Authors: Song Jian , Ren Tingting , Duan Yanheng , Guo Haitao , Wang Gang , Gan Yu , Bai Mengcai , Dong Xiaotian , Zhao Zheng , An Jiaze
Published: March 2023

Background

Near-infrared fluorescence (NIRF) imaging has recently emerged as a promising tool for noninvasive cancer imaging. However, lack of tumor sensitivity and specificity restricts the application of NIRF dyes in surgical navigation.

Methods

Herein, we investigated the imaging features of NIRF dye MHI-148 and indocyanine green (ICG) in live cell imaging and xenograft nude mice models. TCGA dataset analysis and immunohistochemistry were conducted to investigate the expression of OATPs or ABCGs in hepatocellular carcinoma (HCC) tissues. OATPs or ABCGs were knocked down and overexpressed in HCC cells using transient transfection by siRNA and plasmids or stable transfection by lentivirus. Further, qRT-PCR ,Western blotting and the use of agonists or inhibitors targeting β-catenin signaling pathway were applied to explore its important role in regulation of OATP2B1 and ABCG2 expression.

Results

Here we demonstrated that NIRF dye MHI-148 was biocompatible as indocyanine green (ICG) but with higher imaging intensity and preferential uptake and retention in hepatocellular carcinoma (HCC) cells and tissues. Moreover, our data indicated that membrane transporters OATP2B1 and ABCG2, which regulated by β-catenin signaling pathway, mediated tumor-specific accumulation and retention of MHI-148 in HCC cells. In addition, the treatment with β-catenin inhibitor significantly enhanced the accumulation of MHI-148 in HCC tissues and improved the efficacy of tumor imaging with MHI-148 in vivo.

Conclusions

Our study uncovers a mechanism that links the distribution and expression of the membrane transporters OATP2B1 and ABCG2 to the tumor-specific accumulation of MHI-148, and provides evidence supporting a regulating role of the β-catenin signaling pathway in OATP2B1 and ABCG2- induced retention of MHI-148 inHCC tissues, and strategy targeting key components of MHI-148 transport machinery may be a potential approach to improve HCC imaging.