bluuebunny/crossref_metadata_2025 huggingface dataset:
PENERAPAN TEKNOLOGI PADA USAHA BUDIDAYA JAMUR TIRAM UNTUK MENDUKUNG URBAN FARMING DI KWT JAWA GADUT SAIYO LIMAU MANIS PAUH KOTA PADANG
Authors: Darwison Darwison , Zaini Zaini , Aderi Fahranul , Irsan Farhan Fadil
Published: July 2023

Adapun masalah pada kelompok Budidaya Jamur Tiram di KWT Jawa Gadut Saiyo Limau Manis Pauh Kota Padang yaitu harus menjaga suhu dan kelembaban, secara langsung dan terus menerus agar jamur tiram di kumbung berkembang dengan baik. Selain itu juga permasalahan dalam pengisian baglog yang butuh waktu dan tenaga ekstra serta mesin kukus yang masih sederhana. Untuk menyelesaikan masalah tersebut maka diperlukan penerapan teknologi pada usaha budidaya jamur tiram untuk mendukung urban farming dalam hal sistem otomatisasi meliputi aspek suhu dan kelembaban; alat press baglog; wadah kukus baglog; serta sistem monitoring kumbung secara jarak jauh. Hasil yang didapatkan yaitu kelembaban secara otomatis dalam rentang kisaran 80% s/d 92% dan suhu rentang 25oC s/d 29oC. Pembuatan baglog lebih ringan dan hasilnya lebih padat serta hanya membutuhkan waktu 1 menit / baglog. Dan wadah kukus baglog lebih praktis berbentuk lemari yang berkapasitas maksimal 144 baglog.

Kata kunci: suhu, kelembaban, baglog, kumbung, monitoring

ABSTRACT

As for the problem in the Cultivation group Oyster Mushrooms in KWT Jawa Gadut Saiyo Limau Manis Pauh, Padang City, that is, they must maintain temperature and humidity, directly and continuously so that the oyster mushrooms in the kumbung develop properly. Apart from that, there are also problems in filling baglog which requires extra time and effort and the steaming machine is still simple. To solve this problem, it is necessary to apply technology to oyster mushroom cultivation to support urban farming in terms of automation systems covering aspects of temperature and humidity; baglog presses; baglog steam container; as well as remote kumbung monitoring system. The results obtained are humidity automatically in the range of 80% to 92% and temperatures in the range of 25oC to 29 oC. Making baglog is lighter and the results are denser and only takes 1 minute/baglog. And the baglog steamer is more practical in the form of a cupboard with a maximum capacity of 144 baglog.

Keywords: temperature, humidity, baglog, kumbung, monitoring
PENDAMPINGAN PEMASARAN INTERNASIONAL PRODUK MOCAF BAGI PAGUYUBAN SURYA TANI KELOMPOK BINAAN MPM PWM DIY
Authors: Paksi Arie Kusuma , Azizah RR. Zahroh Hayati , Wiratma Harits Dwi , Bhanu Abhipraya Bhanu
Published: July 2023

Paguyuban Surya Tani yang berlokasi di Kabupaten Gunungkidul merupakan kelompok binaan MPM PWM DIY. Paguyuban ini memproduksi dan mengelola berbagai jenis palawija yang kemudian dijual dalam bentuk aslinya, produk setengah jadi, dan produk olahan. Salah satu tanaman yang dikelola dan menjadi mata pencaharian yang mumpuni bagi masyarakat Gunungkidul adalah singkong. Singkong ini mereka olah menjadi tepung serbaguna yang Bernama tepung Mocaf. Pemasaran produk Mocaf dilakukan baik secara offline dan online oleh Paguyuban Surya Tani. Saat ini pemasaran produk Mocaf ini hanya terbatas pada pasar domestik saja, belum menjangkau pasar internasional. Padahal pasar internasional akan lebih banyak peminatnya terhadap produk Mocaf yang merupakan gluten free. Oleh karena itu, perlu adanya dorongan supaya Paguyuban Surya Tani dapat melakukan pemasaran produk untuk dapat menjangkau pasar internasional.

Kata kunci: pemasaran, pasar internasional, Mocaf

ABSTRACT

Paguyuban Surya Tani located in Gunungkidul Regency is a group assisted by MPM PWM DIY. This association produces and manages various types of palawija which are then sold in their original form, semi-finished products, and processed products. One of the plants that is managed and becomes a qualified livelihood for the people of Gunungkidul is cassava. They process cassava into a multi-purpose flour called Mocaf flour. Marketing of Mocaf products is carried out both offline and online by Paguyuban Surya Tani. Currently the marketing of Mocaf products is only limited to the domestic market, not yet reaching the international market. Even though the international market will be more interested in Mocaf products, which are gluten free. Therefore, there needs to be encouragement so that the Surya Tani Association can carry out product marketing to be able to reach international markets.

Keywords: marketing, international market, Mocaf
ANALISIS SENTIMEN PELAKSANAAN KULIAH ONLINE MENGGUNAKAN ALGORITMA SUPPORT VECTOR MACHINE
Authors: Setiana Elia , Marwondo  , Venia Retreva Danestiara  , Wiyanudin
Published: July 2023

This writing aims to assess the satisfaction of students regarding the implementation of online lectures, categorized into three classes: positive, neutral, and negative. Data collection was conducted using Twint from the social media platform Twitter, with a total of 25,000 tweets. The data processing process to determine sentiment analysis utilized the support vector machine algorithm. With this algorithm, the obtained results show an accuracy rate of 76.86% for positive sentiment. The precision is 0.49, recall is 0.53, and the F1 score is 0.51
Pengembangan Aplikasi Keuangan Berbasis Web (Studi Kasus : Mts AT-Taufiq Kota Bandung)
Authors: Fujiwati Lestari Dina Sari , Darsiti
Published: July 2023

Seperti yang kita ketahui bahwa perkembangan teknologi informasi dan komputerisasi membawa dunia ke sebuah era digital. Begitu pula dengan sistem informasi yang terus berkembang dalam keuangan khususnya dalam proses transaksi pemasukan serta pengeluaran kas sekolah dan aplikasi pembayaran SPP hanya memberikan layanan fitur transaksi pembayaran SPP saja sehingga ada beberapa pemasukan dari luar itu tidak terkomputerisasi membuat kinerja dua kali pengerjaan dalam merekap data keuangan yang terpisah dari pembayaran SPP.Teknik pengumpulan informasi dalam riset ini adalah observasi, wawancara dan studi kepustakaan. Setelah itu hasil riset dianalisis memakai metode kualitatif yang ialah proses mencari serta menyusun secara sistematis informasi yang diperoleh dari hasil observasi serta wawancara, dengan metode mengorganisasikan informasi dalam jenis, menjabarkan kedalam unit- unit, menyusun didalam pola, memilah mana yang berarti serta menarik kesimpulan yang mudah dimengerti banyak orang.Pengembangan aplikasi ini menggunakan software sublimtext dan XAMPP dengan menerapkan framework codelgniter dalam Bahasa pemograman PHP dilengkapi rangkaian HTML dan Desain CSS.Hasil dari penelitian ini adalah proses pengelolaan keuangan yang sedang berjalan sekarang dikembangkan menjadi aplikasi keuangan dengan tambahan fitur pemasukan dari luar selain SPP, dan pengeluaran perharinya secara terkomputerisasi di sekolah agar transparan dan juga dapat memudahkan bagian keuangan dalam proses transaksi di sekolah
IMPLEMENTASI ALGORITMA BLUM BLUM SHUB PADA KUIS SANDI SEMAPHORE BERBASIS AUGMENTED REALITY
Authors: Ridwan Alamsyah  , Erlan Darmawan  , Rio Andriyat  , Yulyanto  , Suseno Endra
Published: July 2023

Semaphore is a way to send and receive news by using a flag, paddle, trunk, bare hands or gloves. The semaphore learning at School at this time still uses the Scout's manual and it is only in the form of 2D images and it is also delivered by the Scout coach. So that students tend to get bored in learning the semaphore movement and lack of understanding and education in the learning process in this modern era. From the existing problems, the semaphore movement learning application and semaphore quiz were built using augmented reality technology based on android to provide interesting and interactive learning. This application was built by adding the Blum-Blum Shub (BBS) algorithm for the randomization process which functions to generate random numbers in a mathematical process with the resulting output is a series of binary numbers. Application created uses Unity 3D, and developed uses the RUP (Rational Unifed Process) method. With this application, it can make easier for students to understand the material in semaphore learning
Fabrication of injectable hydrogel derived amnion extracellular matrix
Authors: Nguyen Thi Ngoc My , Lam Minh Hoang , Nguyen Thuan Nho , Tran Le Bao Ha
Published: July 2023

This study was carried out to successfully fabricate hydrogel from decellularized amniotic extracellular matrix (ECM) powder. The pre-gel sample form ECM powder was prepared by pepsin digestion and neutralization process. Hydrogel formation was initiated by incubation at physiological temperature. The inversion test determined that 24-hour dissolving in pepsin solution was optimal for the hydrogel fabrication. Histology examination revealed a three-dimensional fibrin network composed of randomly linked fibers. The hydrogel sample retained its shape in PBS 1X solution. In vitro cytotoxicity showed that the hydrogel liquid extract was safe to the cultured fibroblasts. After 24 hour subcutaneuos injection, pre-gel sample was capable of self-assembling and form hydrogel network under in vivo physiological conditio, indicating the potential application to accommodate irregular defects.
The Effects of Blue and Red Light on the Hill Reaction of isolated Chloroplasts, Photosynthesis and Saponin Accumulation in Fenugreek Leaves (Trigonella foenum-graecum L.)
Authors: Tien Anh Huy Nguyen , Thuong Kiet Do , Trang Viet Bui
Published: July 2023

Blue light (450 nm) and red light (660 nm) were two specific wavelengths that have essential roles in plant photosynthesis and signals to synthesize plant metabolisms. In the fenugreek plant (Trigonella foenum – graecum L.), various saponins were synthesized in leaves and were used in pharmaceuticals. This study focused on the effects of blue and red light alone or in combination with fluorescent light on isolated chloroplasts, photosynthesis activities and saponin accumulation of fenugreek leaves. The results showed that the blue light increased the Hill reaction, stomatal opening and the ratio of chlorophyll a and b (Chla/b). Otherwise, the red light promoted total chlorophyll content and increased saponin in Fenugreek leaves. In addition, the combination of blue, red and fluorescent light increased photosynthesis activities and maintained plant growth and increased saponin accumulation in Fenugreek leaves.
Heterologous Expression, Purification and Characterization of Fusion-type Pediocin PA-1 in Escherichia coli
Authors: Nguyen Pham Anh Thu , Dao Thi Hong Thuy , Nguyen Van Hau , Dang Thi Phuong Thao , Nguyen Hieu Nghia
Published: July 2023

Pediocin PA-1 is a 4.6 kDa bacteriocin that shows strong antimicrobial activity against some Gram-positive pathogens such as Listeria monocytogenes and Enterococcus faecalis. Due to broad inhibitory spectrum as well as pH and temperature stability, pediocin has a potential application in food preservation as well as in the pharmaceutical industry. For higher manufactory efficiency, pediocin has been expressed in some other heterologous expression systems, mostly on Escherichia coli with different strategies. Here, we show a new strategy to produce pediocin from E. coli BL21(DE3) system as a fusion form by using the expression vector pET43.1a which contains the NusA tag. Our results showed that NusA-fused pediocin almost presented in soluble form with high efficiency (79.8 mg/L obtained by Ni-NTA purification). After removing the fusion tag, recombinant pediocin showed antimicrobial activity against L. monocytogenes ATCC 13932 as 23.5×103 AU/mg of specific activity as well as against E. faecalis, Lactobacillus plantarum and Streptococcus thermophilus. Recombinant pediocin was stable at the wide range of pH (1-12 for 60 min) and high temperature (100oC for 15 min) as well as sensitive to protease treatment as the nature pediocin. These characteristics have opened prospects for applying pediocin as a biological preservative in the food industry.
Characterization of Epithelial Stem Cells and Mesenchymal Stem Cells from Human Hair Follicle
Authors: Dao Thi Thanh Thuy , Dang Thi Tung Loan , Nguyen Thi Ngoc Nhu , Phan Lu Chinh Nhan , Nguyen Ton Ngoc Huynh , Tran Le Bao Ha
Published: July 2023

Hair follicle mesenchymal stem cells (HF-MSCs) and epithelial stem cells (HF-EpSCs) play a key role in hair growth and regeneration. The isolation, culture and characterization of HF-MSCs and HF-EpSCs will provide clues for in vitro tissue models and regenerative medicine applications. In this study, HF-MSCs and HF-EpSCs from the hair bulge were harvested using the tissue culture method. HF-MSCs exhibited a fibroblast shape and expressed highly the mesenchymal markers CD44, CD73, CD90 and CD105 and a lack of hematopoietic markers CD45, CD34, CD11b, CD19 and HLA-DR. They showed the potential for differentiation into adipogenic, osteogenic and chondrogenic lineages. HF-EpSCs possessed the keratinocyte shape and expressed typical markers for epithelial stem cells such as CD200, CK15 and CK19. The stemness marker CD49f was preferentially expressed in HF-EpSCs over HF-MSCs. Moreover, the expression of pluripotent factors including c-myc, oct-4, sox-2, klf-4 and nanog at the mRNA level was higher in HF-EpSCs compared to the expression in HF-MSCs. In conclusion, we successfully isolated mesenchymal and epithelial stem cells via culturing the hair follicle bulge tissues and preliminarily evaluated the stemness and plasticity of these two populations. This information will provide clues for further studies on hair regeneration in vitro and in vivo.
Overview of Pending and Dispute Events in the Health BPJS Claiming Process for Inpatients at Imelda Hospital, Medan, Indonesia in 2022
Authors: Valentina Valentina , Simanjuntak Esraida , Lubis Siti Permata Sari , Hulu Linfia Isti Zenika
Published: July 2023

Health insurance is a guarantee in the form of health protection so that participants obtain health care benefits and protection in meeting basic health needs that are given to everyone who has paid contributions or whose contributions are paid by the government. The purpose of this study is to describe the implementation of the BPJS (Social Security Administering Agency) Health claim system for inpatients at Imelda Workers' Hospital in Indonesia. This type of research is qualitative with a case study approach. The method used is observation and direct interviews to the field. The subjects used in this study were all pending and disputed medical record claim files for inpatients and in March 2022 as many as 94 claim files. The informants used in this study were 3 BPJS Health claims officers. Based on the results of the study, the implementation of inpatient BPJS Health claims is in accordance with regulations and SOPs at Imelda Indonesian Workers Hospital in Medan, the obstacles in the claiming process are facilities and networks that often have problems. The claim officer will scan the inpatient file and insert it into the hard disk and send it to BPJS Kesehatan. The hospitalization files that were claimed and submitted in March 2022 were 420 files, of which 326 files were received (78%) and 94 files were rejected (pending claims) (22%). There were also 27 disputed claim files in March 2022 (29%), after the submission of pending claims was made by Imelda Indonesian Workers Hospital in Medan.
Pengaruh Tunjangan Kinerja dan Gaya Kepemimpinan Terhadap Kinerja Pegawai Dengan Motivasi Sebagai Mediator Pada Sekretariat Daerah Kabupaten Sanggau
Authors: Perkasa Bima Yoga
Published: July 2023

Manajemen Sumber Daya Manusia (MSDM) pada prinsipnya diorientasikan pada peningkatan kinerja. Salah satu cara untuk meningkatkan motivasi karyawan adalah dengan memberikan tunjangan yang sesuai dengan pelaksanaan tugas sehingga tercapai kinerja demi terciptanya tujuan organisasi. Penelitian ini bertujuan untuk menganalisa pengaruh tunjangan kinerja dan gaya kepemimpinan terhadap kinerja pegawai dengan variabel motivasi sebagai mediator pada Sekretariat Daerah Kabupaten Sanggau. Dengan jumlah pegawai yang ada. Sekretariat Daerah Kabupaten Sanggau harus dapat memaksimalkan kerja pegawainya untuk dapat memperoleh kinerja yang diharapkan sehingga tugas pokok dan fungsi Sekretariat Daerah dapat berjalan maksimal, hal ini tidak luput dari peran tunjangan kinerja dan faktor kepemimpinan yang tepat untuk memotivasi pegawainya sehingga akan memberikan dampak positif terhadap Reformasi Birokrasi Sekretariat Daerah secara keseluruhan. Teknik pengumpulan data digunakan kuesioner dan teknik analisis data digunakan program smartPLS 3.0. Hasil analisis diketahui bahwa pada Sekretariat Daerah Kabupaten Sanggau: 1) Pengaruh langsung tunjangan kinerja terhadap motivasi pegawai terbukti signifikan, 2) Pengaruh langsung tunjangan kinerja terhadap kinerja pegawai terbukti signifikan, 3) Pengaruh langsung gaya kepemimpinan terhadap motivasi pegawai terbukti signifikan, 4) Pengaruh langsung gaya kepemimpinan terhadap kinerja pegawai signifikan, 5) Pengaruh langsung motivasi terhadap kinerja pegawai signifikan, 6) Pengaruh tunjangan kinerja, gaya kepemimpinan dan motivasi terhadap kinerja pegawai signifikan. Dari hasil analisis perhitungan pengaruh langsung, tidak langsung maupun pengaruh total, dapat disimpulkan bahwa untuk meningkatkan kinerja pegawai agar lebih optimal maka yang harus dilakukan adalah memotivasi pegawai dengan jalan memberikan tunjangan kinerja dan menerapkan gaya kepemimpinan yang baik bagi pegawai.
In silico screening based on chemoinformatics and molecular docking of trypanothione reductase inhibitors from Trypanosoma cruzi
Authors: Hernández Alba Brenda Aylin , Galicia Galicia Erick , Retana Ugalde Raquel , Mendieta Wejebe Jessica Elena , Alvarado Salazar Juan Andres
Published: July 2023

Chagas disease is caused by the parasite Trypanosoma cruzi. One of the two first-line drugs for treatment is nifurtimox, a nitrofuran that exhibits high toxicity. Therefore, the need arises to investigate new therapeutic options, and, in this sense, our objective was to study compounds that maintain the trypanocidal activity associated with the furan nucleus and by structural modification of substituents reduce their toxicity. In the following research work, a chemical library was formed using the databases PubChem, ChEMBL and ChemSpider, later a virtual screening was carried out with the chemoinformatic tools Way2Drug, molinspiration, DataWarrior and admetSAR obtaining the prediction of biological activity, physicochemical parameters, data of toxicity and pharmacokinetic properties, respectively. The last stage consisted of the molecular docking of the molecules at the active site of the trypanothione reductase protein of Trypanosoma cruzi, determining its binding energy and intermolecular interactions. Compounds NF-85, NF-150, NF-246 and NF-279 with potential anti-T. cruzi activity were identified, the four molecules had greater affinity for trypanothione reductase (-7.0, -7.2, -7.0 and -7.3 kcal / mol, respectively), compared to nifurtimox (-6.7 kcal / mol).
Lessons learned from multi-objective automatic optimizations of classical three-site rigid water models using microscopic and macroscopic target experimental observables
Authors: Perrone Mattia , Capelli Riccardo , Empereur-mot Charly , Hassanali Ali , Pavan Giovanni M.
Published: July 2023

The development of accurate water models is of primary importance for molecular simulations. Despite their intrinsic approximations, three-site rigid water models are still ubiquitously used to simulate a variety of molecular systems. Automatic optimization approaches have been recently used to iteratively optimize three-site water models to fit macroscopic (average) thermodynamic properties, providing “state-of-the-art” three-site models that still present some deviations from the liquid water properties. Here we show results obtained by automatically optimizing three-site rigid water models to fit a combination of microscopic and macroscopic experimental observables. We use Swarm-CG, a multi-objective particle-swarm-optimization algorithm, for training the models to reproduce the experimental radial distribution functions of liquid water at various temperatures (rich in microscopic-level information on, e.g., the local orientation and interactions of the water molecules). We systematically analyze the agreement of these models with experimental observables and the effect of adding macroscopic information into the training-set. Our results demonstrate how adding microscopic-rich information in the training of water models allows achieving state-of-art accuracy in an efficient way. Limitations in the approach and in the approximated description of water in these three-site models are also discussed, providing a demonstrative case useful for the optimization of approximated molecular models in general.
Risk Factors for Colonization with S.aureus and Methicillin Resistant Staphylococcus aureus Among Health Care Workers in Al-Batool teaching hospital for maternity and children in Diyala, Iraq
Authors:   Department of Biology, College of Science , University of Tikrit  , Iraq, Mahjoob Aljboori Mohamed , Abbas Abdul Jabbar Rideh , Ibrahim Ali Al-Ezzy Ali
Published: July 2023

Background: Staphylococcus aureus coloniza-tion for the human nose representing a challenge that requires a cope with host defense and competing resident microor-ganisms. Objective: To evaluate the risk factors for in-fection with S. au-reus and MRSA among health care workers (HCWs) in Al-Batool teaching hospital for maternity and children in Diyala, Iraq. Patients and Methods: A total of 27 swabs were taken from HCWs in Al-Batool teaching hospital for ma-ternity and chil-dren in Diyala, Iraq (ATHMC) Standard microbi-ological proce-dures were used for diagnosis of S. aureus and Methicillin Re-sistant Staphylo-coccus aureus (MRSA). Results: Significant corre-lation was report-ed between age and colonization with S. aureus & MRSA. Inverse correlation was reported between education level and colonization with S. aureus and MRSA. Signifi-cant correlation was reported be-tween acne and colonization with S. aureus. Signifi-cant correlation was reported be-tween sinusitis, years of experi-ence, contact with farm animals and colonization with S. aureus and MRSA. Significant correlation was reported between ward of duty and colonization with MRSA. Conclusion: Colonization with S. aureus and MRSA inversely correlated with younger age group, education level of HCWs. Colonization with S. aureus and MRSA correlated with sinusitis, years of experience (5 -6); contact with farm animals. Colonization with S. aureus correlated with acne. Colonization with MRSA correlated with ward of duty at children care floor. Keywords: Health care workers , S.aureus, MRSA ,Risk factors
Resistant Dermatophytosis, the Causative Species, and Treatment
Authors:   College of Medicine, University of Diyala, Diyala , Iraq, Khudhair Khalaf Shahad , Fuad Hussain Anaam , Khalaf Al-Kayalli Khudhair , Khudhair Khalaf Amenah
Published: July 2023

Background: Dermatophytosis was a fungal infection caused by molds (dermatophytes). Dermatophytes are a group of closely related filamentous fungi able to damage and utilize keratin found in the skin, hair, and nails. The present study aimed to isolate the species which cause resistant dermatophytosis, and clinical types of tinea, as well as to assess the response to different antifungal therapies. Patients and materials: ninety-two patients with different clinical types of tinea infections (ringworm) were seen in a private clinic in Baquba city for the period from May 2021 to December 2021, they were (42) females and (50) males, their ages ranged from (7-70) years with a mean age of (27.57±8) years. All patients were diagnosed clinically as ringworm and supported by isolation of the species from samples either by direct examination of samples or cultures on Sabouraud media, and the patients were treated by combination therapy of systemic and topical terbinafine and systemic azole (itraconazole capsules) for (1-3) months. Results: All patients complained of widespread and concomitant tinea. The most common causative dermatophytes species were Epidermopyton floccosum (41%) and Trichophyton rubrum (21%), less commonly Microsporum audouinii (9%), and the others as in table (1). The most common clinical type was tinea pedis (27%) then tinea cruris (24%), tinea corporis (21%), and tinea ungum (19%) as in table (2). All patients were cured on combination therapy of systemic (terbinafine and itraconazole) and topical (terbinafine). Five patients (5%) showed relapses of disease after discontinuation of therapy and retreated by the same method. Conclusion: Epidermophyton floccosum and Trichophyton rubrum were the most common cause of resistant tinea. Terbinafine and itraconazole were good therapeutic options for the treatment of resistant tinea. Key words: Tinea, Dermatophytes, Terbinafine, Itracanazole.
Full-length transcript alterations in human bronchial epithelial cells withU2AF1S34F mutations
Authors: Soulette Cameron M , Hrabeta-Robinson Eva , Arevalo Carlos , Felton Colette , Tang Alison D , Marin Maximillian G , Brooks Angela N
Published: July 2023

U2AF1is one of the most recurrently mutated splicing factors in lung adenocarcinoma and has been shown to cause transcriptome-wide pre-mRNA splicing alterations; however, the full-length altered mRNA isoforms associated with the mutation are largely unknown. To better understand the impactU2AF1has on full-length isoform fate and function, we conducted high-throughput long-read cDNA sequencing from isogenic human bronchial epithelial cells with and without aU2AF1 S34Fmutation. We identified 49,366 multi-exon transcript isoforms, more than half of which did not match GENCODE or short-read–assembled isoforms. We found 198 transcript isoforms with significant expression and usage changes relative to WT, only 68% of which were assembled by short reads. Expression of isoforms from immune-related genes is largely down-regulated in mutant cells and without observed splicing changes. Finally, we reveal that isoforms likely targeted by nonsense-mediated decay are down-regulated inU2AF1 S34Fcells, suggesting that isoform changes may alter the translational output of those affected genes. Altogether, our work provides a resource of full-length isoforms associated withU2AF1 S34Fin lung cells.
Gene-regulation modules in nonalcoholic fatty liver disease revealed by single-nucleus ATAC-seq
Authors: Takeuchi Fumihiko , Liang Yi-Qiang , Shimizu-Furusawa Hana , Isono Masato , Ang Mia Yang , Mori Kotaro , Mori Taizo , Kakazu Eiji , Yoshio Sachiyo , Kato Norihiro
Published: July 2023

We investigated the progression of nonalcoholic fatty liver disease from fatty liver to steatohepatitis using single-nucleus and bulk ATAC-seq on the livers of rats fed a high-fat diet (HFD). Rats fed HFD for 4 wk developed fatty liver, and those fed HFD for 8 wk further progressed to steatohepatitis. We observed an increase in the proportion of inflammatory macrophages, consistent with the pathological progression. Utilizing machine learning, we divided global gene regulation into modules, wherein transcription factors within a module could regulate genes within the same module, reaffirming known regulatory relationships between transcription factors and biological processes. We identified core genes—central to co-expression and protein–protein interaction—for the biological processes discovered. Notably, a large part of the core genes overlapped with genes previously implicated in nonalcoholic fatty liver disease. Single-nucleus ATAC-seq, combined with data-driven statistical analysis, offers insight into in vivo global gene regulation as a combination of modules and assists in identifying core genes of relevant biological processes.
Inhibition of protein arginine deiminase 4 prevents inflammation-mediated heart failure in arthritis
Authors: Heger Lukas A , Schommer Nicolas , Fukui Shoichi , Van Bruggen Stijn , Sheehy Casey E , Chu Long , Rajagopal Sridharan , Sivanandhan Dhanalakshmi , Ewenstein Bruce , Wagner Denisa D
Published: July 2023

Rheumatoid arthritis is a prototypic inflammatory condition with affected patients being at greater risk of incident heart failure (HF). Targeting innate immune cell function in the pathogenesis of HF bears the potential to guide the development of future therapies. A collagen-induced arthritis (CIA) model in DBA/1 J mice was used to generate arthritis. Mice with CIA developed concentric hypertrophic myocardial remodeling, left ventricular (LV) diastolic dysfunction, and HF with elevated plasma B-type natriuretic peptide levels but preserved LV ejection fraction. Key features of HF in CIA were increased infiltration of activated neutrophils, deposition of neutrophil extracellular traps in the myocardium, and increased tissue levels of the proinflammatory cytokine IL-1β. Specific inhibition of protein arginine deiminase 4 (PAD4) by an orally available inhibitor (JBI-589), administered after the onset of clinical arthritis, prevented HF with reduced neutrophil infiltration. We identify PAD4-mediated neutrophil activation and recruitment as the key thromboinflammatory pathway driving HF development in arthritis. Targeting PAD4 may be a viable therapeutic approach for the prevention of HF secondary to chronic inflammation.
Mediating Effect of Perceived Organizational Support on the Resilience and Mental Health of ICU Nurses
Authors: Song Rui , Hao Yu-ying , Li Juan-ni , Yang Xin , Liu Jie , Chen Li-hua
Published: July 2023

Objective: To investigate the mediating effect of organizational support on the relationship between resilience and anxiety-depression-stress by researching ICU nurses. Methods: A total of 675 ICU nurses in Shaanxi Province were surveyed using the organizational support scale, the resilience evaluation scale for medical staff, and the Depression-Anxiety-Stress Scale (DASS-21). Results: The resilience of ICU nurses had significant correlation with the level of organizational support (r = 0.448, P < 0.01), but was negatively correlated with depression (r = -0.238, P < 0.001), anxiety (r = -0.287, P < 0.01), and stress (r = -0.213, P < 0.01); the level of organizational support of ICU nurses was also negatively correlated with depression (r = -0.452,  P < 0.01), anxiety (r = -0.410, P < 0.01), and stress (r = -0.490, P < 0.01). The sense of organizational support played a mediating role between the resilience of ICU nurses and their depression-anxiety-stress, the mediating effect accounted for 78% of the total effect. Conclusion: The perceived organizational support and resilience is an important external protective factor for the mental health and ability of ICU nurses. The management departments should continue to pay attention to the mental health of ICU nurses, take effective measures to improve the sense of organizational support and resilience of nurses, and ensure the good mental health of ICU nurses.
EXPERIMENTALLY CLINICAL CORRELATION OF ALCOHOL-INDUCED CHANGES IN VASCULAR ENDOTHELIAL GROWTH FACTOR
Authors: P.V. Belogubov , V.I. Ruzov , R.R. Sharafutdinova
Published: July 2023

Aim: to comparatively estimate the nature and direction of alcohol-induced changes of vascular endothelial growth factor in the experiment and in the clinic. Materials and methods. The experimental part of the research was performed on 50 outbred mature rats (15 females and 15 males exposed to alcohol intoxication for 30 days, and 10 females and 10 males formed a control group). The average age of males was 38 (37.25–39) weeks, females - 40.5 (40-42.5) weeks. The body weight of females was 340 (329.5–345.5) g, males – 469 (464.5–474.25) g. After fixation, the control group animals were injected with intragastrically sterile 0.9% aqueous sodium chloride solution, the rest of the animals – 40% ethanol daily at the rate of 10 ml / kg, so that the animals reached a mild degree of intoxication. Clinical part of the research included 60 young patients: 30 males and 30 females, mean age was 35,97 (34,00-40,00) in females and 37,5 (34,00-41,00) in males, who were admitted to the narcological hospital for treatment of intoxication with diagnosed alcohol use disorder. The serum concentration of VEGF-A was evaluated in the blood serum of the studied patients and animals. Results. Alcohol intoxication caused an increase of serum concentration of VEGF-A in both animals and patients in comparison with control groups. The changes were unidirectional with no significant gender differences. Conclusion. The analysis of the experimental-clinical comparison of alcohol-induced changes in the serum content of VEGF-A indicates the unidirectional nature of the biological (angiogenic) effect in the form of VEGF-A expression under the action of ethanol. In the clinical interpretation of the physiological or pathological expression of angiogenesis, accompanied by an increase in the serum concentration of VEGF-A, it is necessary to take into account the specifics of the pathological process.
How Do Leaders of State-owned Microfinance Organizations Perceive Their Business Environment?
Authors: Vikentieva E. N. , Andronov M. Yu. , Gagarina M. A.
Published: July 2023

The article is devoted to the study of attitude of representatives of State-owned Microfinance Organizations to their clients and to the regulators as well as the understanding of their own role in these relationships.

The aim of the study was to analyze the State-owned Microfinance Organizations leaders’ perception of the business environment.

The subject of the study includes a subjective assessment of the role of their organization in the financial system of the region, the attitude towards consumers of the services of their organizations (Entrepreneurs), the expected attitude of Entrepreneurs to the services provided by their organizations, and relations with regulatory authorities.

The relevance of the study is associated with the growing importance of State-owned Microfinance Organizations in the implementation of the tasks set by the government of the Russian Federation, so there is a need for an additional study of subjective assessment of their own position in the system of business relations.

The scientific novelty is that the business environment has been structured based on the main participants in the interaction.

The authors used methods of descriptive statistics, in particular, frequency analysis.

As a result, the perception by representatives of state microfinance organizations of their role as participants in the financial market was studied in three aspects of the scheme proposed by the authors: their importance for the economy of the region, the attitude of consumers to their services, and the peculiarities of interaction with regulators in their perception.

It is concluded that representatives of state microfinance organizations assess their field of activity as socially significant and prestigious, the regulator as trustworthy, but admit that some costs for mandatory requirements could be reduced.
The Changing Role of the US Dollar in the Global Monetary System of a Multipolar World
Authors: Ilyinsky A. I. , Magamedov A. S.
Published: July 2023

This research aims to identify possible global monetary system development scenarios in the context of the current United States dollar supremacy.

The primary method used is foresight analysis, which is based on a combination of deductive and historical data and trend analyses. The paper outlines the main drivers of potential change and uses them as inputs for scenario analysis. The three considered scenarios are a continuation of the absolute dollar dominance, reduction of the dollar’s power and split of the world economy between multiple currencies, and the emergence of a new single prevalent currency.

The main conclusion is that the second multipolar scenario resembles the current dynamics the most and is considered the baseline. However, the answer depends on the forecasting horizon as the scenarios have a certain sequencing. The dollar will probably maintain its supremacy in the short term. Its decline in the mid-term will give rise to a multipolar world, which can then, theoretically, lead to the emergence of a new dominant currency in the long term.

The results might be used as a framework for further structured analysis of possible outcomes in this domain.
Mapeamento da Qualidade Ambiental Urbana no bairro Célio Miranda, Paragominas-PA
Authors: Costa Pereira Lucimar , Viana Balbino Maycon , Lima da Silva Tulio Marcus , Lima Correa Denison
Published: July 2023

Esta pesquisa objetivou quantificar, mapear e avaliar, a partir de indicadores ambientais, as condições de qualidade ambiental no principal bairro de uma cidade média localizada na Amazônia brasileira, nos domínios do município de Paragominas-PA. Para tal, aplicou-se uma metodologia de planejamento da paisagem adaptada, com as representações espaciais do uso e ocupação do solo, cobertura vege-tal, temperatura do ar (ºC), umidade relativa do ar (%), dióxido de carbono (ppm) e ruído (dBA) tomadas como atributos ambientais. Os indicadores mostraram que o uso do solo na área de estudo é predominantemente residencial e que há déficit de cobertura vegetal. Níveis elevados de temperatura, CO2 e ruído, e níveis baixos de umidade, contribuíram para um grau de qualida-de ambiental abaixo do satisfatório. De maneira geral, a qualidade ambiental no bairro foi carac-terizada como média, influenciada pela sobreposição de dois ou três atributos negativos, com destaque para o déficit de cobertura vegetal e elevados níveis de dióxido de carbono como atri-butos mais frequentes. A análise da qualidade ambiental permitiu identificar alguns problemas ambientais presentes na área e suas possíveis causas, servindo de subsídio ao planejamento ur-bano do município de Paragominas, principalmente no que tange ao ordenamento territorial. Mapping of urban environmental quality in Celio Miranda, Paragominas-PA A B S T R A C TThis research aimed to quantify, map and evaluate, based on environmental indicators, the conditions of environmental quality in the main neighborhood of a medium-sized city located in the Brazilian Amazon, in the domains of the municipality of Paragominas-PA. For this, a methodology of adapted landscape planning was applied, with spatial representations of land use and occupation, vegetation cover, air temperature (ºC), relative air humidity (%), carbon dioxide (ppm) and noise (dBA) taken as environmental attributes. The indicators showed that land use in the study area is predominantly residential and that there is a lack of vegetation cover. High levels of temperature, CO2 and noise, and low levels of humidity have contributed to a below-satisfactory degree of environmental quality. In general, the environmental quality in the neighborhood was characterized as average, influenced by the overlap of two or three negative attributes, especially the deficit of vegetation cover and high levels of carbon dioxide as the most frequent attributes. The analysis of the environmental quality allowed to identify some environmental problems present in the area and their possible causes, serving as subsidy to the urban planning of the municipality of Paragominas, mainly in what concerns to the territorial ordering.Keywords: Indicators; Negative aspects; Indexes.
A Compact Low-Loss Reflection-Type Harmonic Transponder for Battery-Less RFID Sensors Based on Harmonic Backscattering
Authors: Lee Daju , Oh Juntaek
Published: July 2023

This study presents a compact reflection-type harmonic transponder with a wide input power range for battery-less radio frequency identification (RFID) sensors based on harmonic backscattering. Miniaturizing the circuit size of conventional harmonic transponders is difficult because the input and output matching stages are configured separately, and two antennas are used for each port. The proposed harmonic transponder based on a dual-band matching network matches the load simultaneously at the fundamental and second harmonic frequencies over a wide input power range, thus enhancing the conversion gain (CG) with a compact size. For verification, the proposed transponder is implemented in a compact size with dimensions of 19 mm × 17.8 mm. The measured CG of the implemented transponder is maintained at over −10 dB in the wide input power range of −6.4 dBm to 10.6 dBm at 2.45 GHz. Th e harmonic transponder is configured with a dual-band chip antenna and measured in free space to verify whether it is suitable for battery-less RFID sensors. The measured detectable effective distance to the proposed circuit is 6.3 m in free space, with an equivalent isotropically radiated power of 42.6 dBm.
Synthesis, characterization, molecular docking, and in vitro antidiabetic activity studies of new and highly selective methoxy-substituted benzimidazole
Authors: Athimoolam T. , Devaraj Stephen L. , Gunasekaran B. , Krishnamurthi J.
Published: July 2023

The benzimidazole-based new therapeutic agent has been efficiently synthesized using one pot condensation-cyclization reaction method involving 1,2-phenylenediamine, and benzaldehyde under mild condition and characterized by different analytical tools such as NMR, Mass, and FT-IR spectroscopy. This compound has employed a protein inhibition assay for the in-vitro antidiabetic and anti-inflammation activity. The obtained data are compared with standard drug molecules. This activity has been correlated with molecular docking studies with three protein molecules for diabetes and inflammations. The structure of the compound was confirmed by the single-crystal XRD method. In addition, the theoretical method is also used as supporting data for its activity towards diabetic and inflammatory activity. The FMO, MEP, and Mulliken charge distribution have been made using DFT analysis, and various reactive parameters are calculated using HOMO and LUMO. The benzimidazole-based TBTPBI has experimented with antidiabetic and anti-inflammatory activity in-vitro manner using protein inhibition and denature techniques. A perfect correlation was found between DFT and the biological screening of TBTPBI with less binding energies and higher inhibition constants values.
Initiation of Prevention of Violence against Women and Children through Community-Based Resilience Strengthening in Pedak, Srandakan, Bantul
Authors: Arini Merita , Dirwan Suryo Soularto R. , Iring Primastuti Harumi
Published: July 2023

Violence against women/children (VaW/C) has bio-psycho-socio-economic impacts. The Pedak community has not been exposed to efforts for violence prevention, although some VaW/C cases have arisen. Hence, this community service aimed to increase public awareness to prevent and reduce VaW/C. The approach Community-Based Resilience is carried out through a cycle of action research, including diagnosing, planning, action, and evaluation in Padukuhan Pedak, Trimurti, Srandakan, Bantul, Yogyakarta Province.  Observation of the community conditions and in-depth interviews with community leaders were used in the diagnosing stage. The action stage includes three priority actions: VaW/C education for community representatives (a quasi-experiment), VaW/C education in children's reading corners, and youth Posyandu coaching. These activities increased public knowledge and reactivated community activities to prevent and overcome societal violence. Multisector role involvement and a multifaceted approach are needed to increase community awareness of VaW/C and assist them in designing applicable and sustainable programs.
Perspectives of Intensive Care patients and family members on competencies for Advanced Intensive Care nurses in Europe
Authors: Jones Christina , Peskett Mo , Ramsay Pam , Endacott Ruth , Xyrichis Andreas , Iliopoulou Katerina
Published: July 2023

Background

One output from the International Nursing Advanced Competency-based Training for Intensive Care (INACTIC) collaboration is a set of core competencies for advanced practice Intensive Care Unit (ICU) nurses across Europe. Some European countries, such as the UK, have identified such competencies, however, these advanced practice roles are rarely practiced across the rest of Europe. The INACTIC competencies were developed with an expert panel of 184 ICU nurses from 20 countries. It is also important to examine what patients and relatives with experience of intensive care felt about these competencies.  

Aim

To examine the views of recovered ICU patients and relatives regarding the INACTIC competencies.

Methods

Three patient and relative focus groups were conducted in England (n=5), Scotland (n=4) and Greece (n=4) to discuss a lay version of the INACTIC competencies. Discussions were open ended, followed a topic guide, recorded and transcribed verbatim. Analysis followed a conventional thematic approach, with the findings discussed iteratively among the authors.

Results

The feedback from across the focus groups resulted in three themes: 1) the importance of nurses being empowered to advocate for the patient; 2) the centrality of communication; and, 3) the impact of variability in ICU practices. There was a notable difference with the Greek focus group; because of restricted family visiting policies, relatives did not feel encouraged to participate in patient care.

Conclusions

The perspectives of patients and relatives largely aligned with the consensus of the INACTIC expert panel. Local differences in ICU experience highlight the changes that some ICUs would need to make for the INACTIC competencies to be embedded.
Effect of a “Do Not Resuscitate” (DNR) Status on Patient Care: A Descriptive Survey on the Perceptions of ICU and Medical/Surgical Nurses
Authors: Rainbolt Clemente Joria , Hess Patrick , Oca Cyr , Raphael Mary , Valdez Regina , Cohn Felicia , Rondinelli June
Published: July 2023

Background: Nursing research literature demonstrates a persistent concern regarding how nurses interpret “Do Not Resuscitate” (DNR) code status.
Aim: The aim of this study was to assess nurses’ perceptions of the effect of a DNR code status on patient care.
Methods: This study was a cross-sectional, descriptive survey offered to all clinical nurses in the Medical, Surgical, Telemetry, Critical Care, and Emergency Departments within one urban community hospital in Southern California, USA.  Descriptive statistics were used for demographic data and individual question analysis, which included frequency and percentages for categorical variables in addition to means with standard deviation for continuous data. Open- ended questions received content analysis.
Results: This study resulted in a convenience sample of 120 registered nurses (RNs). Greater than 95% of nurses who completed the survey agreed that nursing care should continue until patient death.  Yet when asked about physiologic, demographic, chronic, or cognitive status, participants answered with varying degrees of certainty about the care that should be provided.
Conclusions: Further research is needed to explore the inconsistency in nurses’ understanding of the care provided to a patient with DNR status. There may be opportunities to improve consistency in practice with education or protocols to outline optimal care of a patient with DNR orders.