bluuebunny/crossref_metadata_2025 huggingface dataset:
ANTIOXIDANTS AND FREE RADICALS IN HUMAN HEALTH AND DISEASE
Authors: Halliwell Barry
Published: September 2024

Increased damage by ROS plays a role in the development of neurodegenerative diseases, especially Alzheimer’s Disease and other dementias, and diets rich in antioxidants (high intake of fruits and vegetables) seem neuroprotective (as well as being protective against many other age-related diseases). However, attempts to treat/prevent such diseases by giving high doses of antioxidants such as vitamins E and C and carotenoids have, overall, been unsuccessful. Reasons for this will be discussed. A major focus of our work is a unique diet-derived thiol/thione with antioxidant properties, namely ergothioneine (ET). Low blood levels of ET are a risk factor for the development of neurodegenerative and cardiovascular diseases, frailty, eye disease, pre-eclampsia and age-related diseases generally. We have identified “adequate levels” of plasma ET in humans, levels below which are associated with increased disease occurrence, and the reasons leading to these low levels are under investigation. In animal studies, ET has exhibited the ability to modulate inflammation, scavenge certain ROS, protect against acute respiratory distress syndrome, decrease brain damage in models of Parkinson and Alzheimer diseases and stroke, prevent endothelial dysfunction, protect against ischemia-reperfusion injury, counteract iron dysregulation, hinder lung and liver fibrosis, and mitigate damage to the lungs, kidneys, liver, gastrointestinal tract, and testis. ET may also influence the gut microbiome. There is evidence that ET is specifically accumulated at sites of tissue injury, so we have called it an “adaptive antioxidant” that may not interfere with the normal physiological roles of ROS. But does low ET predispose to age-related diseases or is it a spurious correlation? Extensive cell and animal studies strongly suggest the former. Caveats in the use of ergothioneine supplements to prevent/ameliorate aged-related diseases include its potential to generate trimethylamine-N-oxide by the action of ergothionase enzymes in gut bacteria and its ability to be taken up by many bacteria, a few of which are pathogenic (e.g. H. pylori, M. tuberculosis). These caveats will be discussed.
TRIAL READINESS IN MITOCHONDRIAL MEDICINE
Authors: Mancuso Michelangelo
Published: September 2024

Mitochondrial diseases, characterized by dysfunction in the cellular powerhouse, the mitochondria, present a complex and heterogeneous group of disorders. As research in mitochondrial medicine advances, the need for effective therapies becomes increasingly apparent. Collaborative efforts among researchers, clinicians, regulatory bodies, patient advocacy groups and other stakeholders are crucial to overcome the challenges linked to the complexity of mitochondrial medicine, and to ensure the successful implementation of clinical trials in this field. This lecture explores the key aspects of trial readiness in the context of mitochondrial medicine, emphasizing the challenges and opportunities in designing and executing successful clinical trials. An overview of the ongoing clinical trials will be also provided.
HEPATOCYTE SEIPIN SILENCING REDUCES CHOLESTEROL-MEDIATED LIPID DROPLET MATURATION IN FATTY LIVER MODEL
Authors: Demirel-Yalciner Tugce , Cetinkaya Bengu , Sozen Erdi , Kartal Ozer Nesrin
Published: September 2024

The incidence of non-alcoholic fatty liver disease (NAFLD) is gradually increasing with the prevalence of obesity, which is the strongest risk factor for steatosis. Lipid droplet (LD) accumulation in hepatocytes is a hallmark of NAFLD. Seipin protein, which is LD related protein, resides in the endoplasmic reticulum membrane and a shortage of this protein leads to accumulation of abnormal LDs in adipose tissue. Although it has been shown that adipose-specific Seipin deficiency causes increased lipid accumulation in liver and muscle tissue following abnormal LD formation and loss of adipose tissue function, Seipin protein deficiency in liver tissue and its effect on lipid accumulation have not been investigated. Our study aimed to investigate the effect of Seipin deficiency on ER stress and lipophagy in cholesterol-accumulated mouse hepatocyte cells (AML12 cell line). In this direction cholesterol accumulation in mouse hepatocyte cells was established by administrating cholesterol-containing liposome and Seipin levels were reduced using siRNA transfection. Following liposome-cholesterol and siRNA administrations, lipophagy was determined by confocal microscopy, and mRNA levels of GRP78, GRP94, and ATF4 were examined by qRT-PCR. Our findings show that cholesterol-containing liposome administration in hepatocytes increases both Seipin protein and number of large LDs. However, Seipin silencing reduced the increase of cholesterol-mediated large LDs and GRP78 mRNA. Additionally, lysosome-LD colocalization increased only in cells treated with cholesterol-containing liposome, while the siRNA against Seipin did not lead to any significant difference. According to our results, we hypothesise that Seipin silencing in hepatocytes reduced cholesterol-mediated LD maturation as well as GRP78 levels, but not lipophagy.
NRF2/AMPK AXIS IS REQUIRED FOR REDOX-MEDIATED PHASE RESETTING OF MUSCULOSKELETAL CLOCKS UPON ACUTE MECHANICAL LOADING
Authors: Ersoy Ufuk , Winstanley-Zarach Phaedra , Poulet Blandine , Pekovic-Vaughan Vanja
Published: September 2024

In mammals, a multi-oscillator circadian system generates behavioural, metabolic and physiological ~24h rhythms, with tissue-specific physiological cues enabling local circadian phase adjustments. Emerging work has shown musculoskeletal tissue homeostasis and mechanical responses to be under circadian control. Nuclear factor erythroid 2-related factor 2 (NRF2), a master regulator of the antioxidant response, is a clock-controlled target in several peripheral tissues and also modifies circadian gene expression and rhythmicity. However, the role of NRF2 in mechanical loading-induced changes in musculoskeletal tissues has yet to be elucidated. Wild-type (WT) and Nrf2 KO mice of young (3-6m) or old age (18-20m) harbouring a PER2::luciferase clock reporter were subjected to acute mechanical joint loading of the right leg (peak load 9N, 40 cycles of 10sec) during light phase whilst the contralateral (left) leg served as a non-loaded control. Musculoskeletal tissues were collected for analysis 4 hrs later. Real-time bioluminescence imaging of clock gene reporter activity, protein and mRNA levels of target markers, NRF2/ARE transactivation and genome-wide RNAseq analyses were undertaken. We show that acute mechanical loading in WT mice led to a decrease in gene expression of key members in the negative and auxiliary feedback loops of the molecular clock, associated with the phase-resetting of PER2::luc protein oscillations in the skeletal muscle and a knee joint. This was accompanied by a significant increase in the markers of oxidative burden as well as gene expression and protein abundance levels of antioxidant enzymes. Moreover, acute mechanical loading induced a significant activation of the redox-sensitive energy sensor, AMP-activated kinase (AMPK), known to be involved in molecular clock resetting. We thus examined whether the above acute mechanical responses were dependent on NRF2 activity. Nrf2 KO mice showed an altered response to acute mechanical loading, characterized by blunted circadian resetting and antioxidant responses, and altered AMPK activation. Furthermore, dampened responses to acute mechanical signals were found in ageing WT mouse musculoskeletal tissues, whilst AMPK activator treatments in WT mice induced circadian resetting and antioxidant responses in an NRF2-dependent manner. In conclusion, these data demonstrate that AMPK/NRF2 axis is required for relaying acute mechanical signals to the musculoskeletal system by controlling redox-mediated phase resetting of musculoskeletal clocks and antioxidant protection, which have important implications in understanding biomechanical mechanisms involved in musculoskeletal tissue maintenance in health and with ageing.
MULTIMODAL IMAGING OF CELLULAR SENESCENCE – OXIDIZED LIPIDS AND ENZYMATIC ADAPTATIONS IN AGING SKIN AT THE SINGLE CELL LEVEL
Authors: Kremslehner Christopher , Narzt Marie Sophie , Zoratto Samuele , Sochorová Michaela , Nagelreiter Ionela Mariana , Gendronneau Gaelle , Marcato Francesca , Tessier Agnes , Ponweiser Elisabeth , Haschemi Arvand , Marchetti-Deschmann Martina , Gruber Florian
Published: September 2024

Changes in carbohydrate metabolism are a key feature of aging which also manifest in the epidermis. Furthermore, the synthesis and distribution of epidermal lipids changes with age. Both these parameters cannot be investigated with immunohistochemistry, as neither serves as useful epitope. We developed a multimodal analytical histocytometry approach combining modalities that localize lipids and enzymatic activities with immunofluorescent imaging of the skin to localize changes that are correlated with appearance of senescent cells. The activities of key metabolic enzymes were determined on tissue sections of aged and juvenile skin with a formazan-based assay. Lipids were localized and quantified using FTICR MALDI - mass spectrometric imaging. We correlated those modalities with immunofluorescent imaging and analyzed the intensities of the respective signals at single cell level, using Strataquest tissue cytometry. We analyzed skin from donors of young (< 30 y) versus advanced (> 67 y) ages and we investigated epidermal equivalent models containing labeled UV-damaged or senescent keratinocytes. Enzymatic activities displayed specific patterns across the stratifying epidermis, and had diverging trajectories in aging, with a marked decrease in suprabasal glucose-6-phosphate dehydrogenase (G6PD) activity. G6PD, the rate limiting enzyme of the pentose phosphate pathway was also identified as a rapid response pathway activated upon UV damage in the epidermis.  The lipid molecular imaging identified differentiation- and age-related changes of polar lipids in skin biopsies and epidermal equivalents, and pro-senescent stress dependent reactive aldehydophospholipid species in the basal epidermal layers.  While these methodologies are still in development, it is evident that correlative analytical imaging – with the aid of AI driven histocytometry – will continue to yield novel insights into skin and epidermal biology by localizing previously undetectable parameters within the epidermis in the context of aging.
NOVEL TARGETED VIOLOGEN FOR THE INDUCTION OF SUPEROXIDE PRODUCTION IN MITOCHONDRIA
Authors: Juric Matea , Resende Bruna Rafaela Pereira , Pant Tarun , Sikora Adam , Hardy Micael , Zielonka Jacek
Published: September 2024

Mitochondrial production of O2•– and H2O2 has been implicated in redox signaling and in the pathogenesis of numerous diseases including cancer, neurodegeneration, and cardiovascular diseases. To understand the exact role of those species, new chemical biology tools for selective and efficient induction of mitochondrial superoxide production are needed. Here, we report the development of a new viologen-based redox cycling agent, mito-diquat (Mito-DQ), capable of inducing targeted mitochondrial O2•– production at significantly higher rates as compared to previously reported mito-paraquat (Mito-PQ), a widely used chemical tool to study mitochondria-dependent redox signaling.
BLOOD REDOX STATUS IN DIFFERENT HUMAN PATHOLOGIES
Authors: Pincemai Joël , Defraigne Jean-Olivier , Cheramy-Bien Jean-Paul , Sakalihasan Natzi , Christelbach Sophie , Le Goff Caroline , Laoudj-Chevinesse Dalila , Maury Jonathan , Rousseau Anne-Françoise , Cavalier Etienne
Published: September 2024

The in vivo determination of oxidative stress always remains a great challenge. Our approach in Liège CHU consists of simultaneously measuring in blood samples four different kinds of biomarkers: enzymatic and non-enzymatic antioxidants, trace elements, markers of oxidative damage to lipids, and identification of sources leading to increased reactive oxygen species (ROS) production. All these biomarkers (n = 16) have been investigated in patients: 1) with Abdominal Aortic Aneurysm (AAA)1 or operated for Thoracic Abdominal Dissection (TAD)2, 2) suffering from Chronic Obstructive Pulmonary Disease (COPD)3 or FacioScapuloHumeral Myopathy (FSHM)4, 3) with COVID-195,6 and 4) with delirium7. When compared to our internal reference values, depletion in non-enzymatic antioxidants (vitamin C, β-carotene, vitamin C/vitamin E ratio, thiol proteins) and trace elements (zinc, selenium) was observed in the majority of these pathologies. By contrast, increased levels in glutathione peroxidase, copper/zinc ratio, lipid peroxides (ROOH), and myeloperoxidase are common in all these diseases.
APPLICATION OF FREE RADICAL SCAVENGERS IN HUMAN LUNG CANCER CELLS IRRADIATED WITH PHOTONS AND CARBON IONS
Authors: Petković Vladana , Keta Otilija , Đorđević Miloš , Petringa Giada , Cirrone Pablo , Petrović Ivan , Ristić Fira Aleksandra
Published: September 2024

Ionising radiation damages DNA directly, or indirectly, causing water radiolysis and formation of free radicals. Indirect irradiation effects could be diminished in the presence of free radical scavengers, such as dimethyl sulfoxide (DMSO). Such conditions would allow the evaluation of direct radiation effects and provide a better understanding of cellular response to irradiation-induced damages. The goal of this study was to investigate the effects of low (γ-rays) and high linear energy transfer (LET) radiation (carbon ions) in non-small lung cancer cells HTB177. Cells were pre-treated with DMSO and irradiated with 60Co γ-rays and 62 MeV/u carbon ions, with doses ranging from 1-5 Gy. Results obtained by clonogenic survival and γ-H2AX foci assay showed that DMSO increased cell survival and decreased number of DNA damages, which points to radioprotective effect of DMSO. The contribution of direct and indirect radiation effects was estimated by the degree of protection (DP) in presence of DMSO. The values of DP rose in a concentration-dependent manner in all irradiated samples. In cells irradiated with γ-rays, 35% of damages were caused directly, while 65% of lesions could be attributed to indirect radiation actions. In presence of carbon ions, contribution of direct effects was 49%, while 51% of damage resulted from indirect radiation effects, showing that free radicals attain an important role in both low and high LET irradiations. The obtained results showed that DMSO can be used as a free radical scavenger to examine the direct and indirect effects on human cancer cells. The numerical Monte Carlo simulations allow modelling of direct and indirect irradiation actions in cancer cells with photons and hadrons. Therefore, this data will be used for validation and further improvement of numerical simulations in comparison to the data collected on different cell lines and irradiation energies, with the goal to improve therapeutic protocols.
UNCOUPLING PROTEIN 1 EXPRESSION IN LIPOMA TISSUE AND LIPOMA-DERIVED STEM CELLS
Authors: Stojanovic Sanja , Korac Aleksandra , Najman Stevo , Jankovic Aleksandra
Published: September 2024

Mechanisms and factors that lead to the formation of lipomas, benign tumors of adipose tissue, are still insufficiently elucidated. Mesenchymal stem cells (MSCs) isolated from lipomas have some similar characteristics to MSCs isolated from white adipose tissue but differ at the molecular level and in their differentiation potential. Considering histological appearance of lipomas, it is not clear to what extent lipomas share common characteristics with other adipose tissue type, brown adipose tissue. Therefore, the aim of this study was to examine the level of uncoupling protein 1 (UCP1), a marker of brown adipose tissue, expression in lipoma tissue as well as in MSCs isolated from lipomas, i.e. lipoma-derived mesenchymal stem cells (LDSCs). LDSCs were grown in standard cell culture conditions and subjected to adipogenic differentiation. UCP1 expression was examined at the RNA level, using Real-Time PCR, and at the protein level, using immunohistochemistry and immunogold staining. Expression of UCP1 in lipoma tissue and LDSCs was compared with the expression of UCP1 in subcutaneous white adipose tissue (scWAT) and adipose-derived mesenchymal stem cells (ADSCs) grown and differentiated in the same cell culture conditions. Differences were observed in UCP1 expression at both RNA and protein levels in lipomas compared to scWAT directing the future research towards the potential of browning mechanisms of adipose tissue involved in lipoma tissue formation.

This research was financially supported by the Science Fund of the Republic of Serbia, PROMIS, #6066747, WARMED and the Ministry of Science, Technological Development and Innovations of the Republic of Serbia, Contract No. 451-03-65/2024-03/200113.
TUMOR SIZE AS THE BEST PREDICTOR FOR THE PRESENCE OF BREAST CANCER METASTASES IN AXILLARY LYMPH NODES
Authors: Drvendžija Zorka , Udicki Mirjana , Zakić Tamara , Janković Aleksandra , Srdić Galić Biljana , Korać Aleksandra , Korać Bato
Published: September 2024

The metastasis of breast cancer to the axillary lymph nodes represents a crucial aspect of disease progression and prognostic evaluation. The presence of metastases in the axillary lymph nodes is a key indicator that breast cancer is in an advanced stage, which can influence the therapeutic approach and the patient's prognosis. For this reason, we conducted a study aimed at examining the factors that contribute to the presence of metastases in lymph nodes in our female population. This research represents a prospective study conducted at the Institute of Oncology of Vojvodina in Sremska Kamenica. The study included 72 female participants diagnosed with breast cancer who underwent surgery at the Institute of Oncology of Vojvodina and had not received preoperative chemotherapy or radiation therapy. Initially, anamnestic data were collected from the participants, followed by a pathohistological analysis of the tumor tissue samples, including immunohistochemical analysis. We examined the influence of age, tumor size, activity of estrogen, progesterone, and HER2 receptors (human epidermal growth factor receptor-2)  in tumors, as well as the occurrence of menarche and breastfeeding duration, on the presence of metastases in axillary lymph nodes. The results of binary logistic regression showed that the only significant predictor for the presence of metastases in axillary lymph nodes was tumor size (p=0.01, Wald=6.57, and Exp(B)=1.11), while the other examined predictors were not statistically significant (p>0.05). In our study population, the size of the breast cancer was crucial for the presence of metastases in the axillary lymph nodes.

This research was supported by the Science Fund of the Republic of Serbia, #7750238, Exploring new avenues in breast cancer research: Redox and metabolic reprogramming of cancer and associated adipose tissue - REFRAME.
IS REDOX-HYPERACTIVITY IN EXTREMOPHILIC MICROALGAE LINKED TO THEIR INCREASED METABOLIC BURDEN?
Authors: Vesković Ana , Dimitrijević Milena , Kovačević Snežana , Spasojević Ivan , Popović Bijelić Ana
Published: September 2024

The diverse uses of microalgae in ecological remediation, wastewater treatment, pharmaceutics, or food and biofuel production, have long kept these single-celled organisms in the spotlight. The focus of this study was on Chlamydomonas acidophila strain PM01, which thrives in acidic aquatic systems and is resistant to the presence of heavy metals in its environment. The redox metabolism of this microalga was assessed by its ability to reduce the EPR-active spin probe TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl), and compared to that of Chlorella sorokiniana strain CCAP 211/8K, a freshwater green microalga. The results showed that C. acidophila has a faster redox metabolic rate than C. sorokiniana, reducing 50% of TEMPO after 2.5, and 13 min, respectively. The addition of Mn2+ or Fe3+ to the culture medium of C. acidophila did not affect its reduction capacity, while it had a minor effect on C. sorokiniana. The faster rate in C. acidophila most likely represents the result of its adaptation to acidic environments. Namely, it has previously been suggested that acidophilic algae perform energy-demanding cellular processes in order to cope with the high pH gradient across the membrane. Moreover, the increased metabolic turnover requires an increased mitochondrial activity, resulting in a higher baseline production of superoxide and hydrogen-peroxide, subsequently compensated by an elevated baseline reduction capacity. Interestingly, the redox metabolic rate of C. sorokiniana was unaltered in suspensions that were kept in non-standard cultivation conditions (diurnal fluctuations of temperature and ambient lighting, absence of shaking) for five weeks. However, C. acidophila lost all of its reduction capacity in these conditions already after three days. These findings may be important when selecting the most appropriate microalgal strain for a specific application. Specifically, C. acidophila would likely be a good candidate for high-yield rapid production of endogenous products that are the result of its unique survival mechanism under extreme conditions.
ARTIFICIAL LIGHT DISRUPTS NATURAL DAY/NIGHT VARIATION IN ANTIOXIDANT SYSTEM OF TREE FROG (HYLA ARBOREA)
Authors: D Prokić Marko , P Gavrić Jelena , G Despotović Svetlana , R Gavrilović Branka , B Radovanović Tijana , Kijanović Ana , Mirč Marko , Vukov Tanja , Đukić Nevena , Tomašević Kolarov Nataša , G Petrović Tamara
Published: September 2024

One of the adaptive features that organisms developed throughout their long natural history is the ability to change the activity of cells, tissues, and organs on a daily, 24-hour, basis. These cyclical changes are synchronized to the external environment through a light-dark regime and internal circadian clock. Daily recurring environmental changes are followed by variations in animal behavior and physiology, which include oscillations in neuroendocrine, metabolic, cardiovascular, and immune functions. By transforming the circadian periodicity of day, artificial light from anthropogenic sources might interfere with organisms leading to a disturbance in hormone levels and physiological stress. In this study, we investigated daily natural variations in the antioxidant system and the effects of artificial light on the redox balance in larvae of tree frogs. We compared antioxidant parameters in tadpoles from the natural day/night cycle (control) with ones exposed to artificial light at night (treatment). The antioxidant response was measured at four time points during 24h (morning, day, evening, and night). Our results showed that only GR activity did not display day/night changes nor was affected by night illumination. For GSH-Px and GST we reported changes in activity at different times of day that were in the same manner for both treatment and control. The highest values were in the morning compared to the other time points. Variation during 24h was also observed for SOD, CAT and GSH. However, exposure to night light affected the pattern and intensity of these parameters compared to the control group. Overall our study suggests that daily differences in metabolic activity can result in variations in the antioxidant system and that the presence of artificial light affects these changes. The disrupted natural rhythm of the antioxidant response may further reflect on other physiological processes and lead to a state of oxidative stress.
First record of Petelia distracta in Uttar Pradesh, India, and the novel observation of puddling behavior in (Geometridae:Ennominae) from India
Authors: Sheikh Taslima , Pandey Ratindra , De Rupak , Khan Abu Arshad
Published: September 2024

This study documents the first occurrence of Petelia distracta (Walker, 1860) in Uttar Pradesh, India. Additionally, it reports the unprecedented behavior of these moths engaging in nocturnal puddling on cow dung and wet old leaf. The observations contribute to the understanding of puddling behavior in Geometridae for the first time in India, offering new insights into the ecological interactions and resource utilization strategies of nocturnal Lepidoptera.
Active Video/Arcade Games (Exergaming) and Energy Expenditure in College Students.
Authors: Siegel Shannon , Haddock Bryan , DuBois Andrea , Wilkin Linda
Published: September 2024

Video games have become increasingly popular among young adults. The purpose of this pilot study was to determine if interactive video/arcade games, requiring physical activity to play, increase the energy expenditure (EE) and heart rate (HR) of young adults enough to elicit a training response. Thirteen male and female participants 26.6 ± 5.7 years of age were in the study. Participants were familiarized with equipment and allowed to practice with three games: (1) moving and striking lighted pads, (2) riding a bike to increase the pace of a race car, and (3) boxing against a video simulated opponent. A portable metabolic cart and HR monitor were attached to participants to measure baseline and exercise values. Participants could play any of the three games for 30 minutes while metabolic and HR data were collected. Exercise data were compared to baseline measures, and the 3 games were compared for EE. Paired sample t-tests showed baseline and exercise values differed for HR (t(12) = -18.91, p < 0.01), and EE (t(12) = -15.62, p < 0.01). The boxing game provided the highest VO2 (17.47 ± 4.79 ml·kg·-1min-1). Participants achieved 60% or better of their HR reserve (162.82 ± 10.78 beats·min-1), well within the ACSM guidelines for a training HR. Caloric expenditure during the 30-minute exercise session (226. 07 ± 48.68) is also within the ACSM recommendations for daily physical activity. Thus, interactive video/arcade games that require physical activity to play can be utilized as part of an overall aerobic exercise program.
Guarantee of Access to Land and Maintenance of the Amazon Forest: The Case of Extractive Reserves
Authors: Machado Ferreira Fernanda , Ferreira Neto José Ambrósio , Benitez Romero Flora Magdaline , Hummel do Amaral Cibele , Paula de Almeida João Victor , Cerruto Ribeiro Sabina
Published: September 2024

The creation of Protected Areas (PAs) has been a global strategy for the preservation and conservation of natural resources, increasingly threatened by human action. In Brazil, from the 1990s onwards, the incorporation of the sociocultural perspective into the creation of PAs was verified, through the creation of Sustainable Use Conservation Units, among them the Extractivist Reserves (Resex), a Protected Area category created from the struggle of traditional populations to guarantee the maintenance of their ways of life. Thus, we analyzed the dynamics of land use and cover of the territory in the five Resex in the state of Acre, Brazil, in order to verify whether the permanence of families living in these areas is associated with low rates of deforestation. Information on land use and cover classes from the MapBiomas Project was used for a multitemporal analysis of forest loss. The results show that the forested area represents 98.7% of the totality of these territories, in addition to revealing the absence of significant changes in the use and cover of these territories. Deforestation totaled 23.5 thousand hectares from the creation of the Resex until 2017, which represents only 0.8% of the total area analyzed. It was found that the Resex are effective for the conservation of the Amazon Forest, capable of reconciling the ways of life of the traditional populations that inhabit these territories with low rates of deforestation.
Pelatihan Kerajinan Pot Lukis dan E-Marketing Sebagai Sarana Pemberdayaan SDM Masyarakat di Kelurahan Lontar Surabaya
Authors: Rais Anisa Rosa , Fauziah Ike Nur , Yudha Amriel Egan Evanzha
Published: September 2024

Pot lukis merupakan salah satu produk UMKM di Kelurahan Lontar, Sambikerep, Surabaya yang sedang berkembang pesat karena kerajinan tersebut tinggi nilai seni dan nilai guna nya sehingga membuat banyak orang tertarik untuk membeli bahkan ingin belajar membuatnya. Selain tinggi nilai seni dan nilai guna produk tersebut juga menerapkan sistem 3R (Reduce, Reuse, Recycle) yang dimana dapat mengurangi limbah plastik. Namun, banyak pengrajin pot lukis yang belum mampu memasarkan produk tersebut secara digital melalui marketplace sehingga produk tersebut belum dikenal secara luas oleh masyarakat Surabaya melainkan hanya masyarakat Kelurahan Lontar saja. Hal tersebut dikarenakan banyak masyarakat yang belum memahami pengguanaan marketplace untuk memasarkan produk kerajinan pot lukis secara online. Tujuan dalam kegiatan pelatihan ini dapat membantu masyarakat Kelurahan Lontar dalam mengolah limbah gallon plastik menjadi suatu kerajinan tangan yang bernilai jual yaitu pot lukis serta pemanfaatan teknologi internet di bidang E-Marketng untuk memasarkan produk pot lukis secara luas. Metode yang digunakan dalam melakukan pengabdiam masyarakat ini adalah Participatory Rural Appraisal (PRA). Sebelum kegiatan tersebut diselenggarakan seluruh panitia melakukan evaluasi pelaksanaan kegiatan, penentuan lokasi, dan penentuan peserta pelatihan. Terdapat 15 orang peserta dalam pelatihan tersebut yang sebagian besar merupakan ibu-ibu PKK Kelurahan Lontar.
Pelatihan Pembuatan PMT (Pemberian Makanan Tambahan) untuk Ibu dan Balita di Desa Lemahkembar, Kabupaten Probolinggo
Authors: Ristia Sari Anggita , Fatmawati Febri , Xaviera Brigette Lee , Musthofa Muhammad Rifqi , Kusuma Ariesa Maryam , Edo Wahyudi Kalvin
Published: September 2024

Indonesia menjadi salah satu negara yang memiliki angka stunting yang tergolong cukup tinggi, terutama pada balita yang harus mendapatkan perhatian khusus dikarenakan usia-usia balita merupakan masa-masa yang sangat penting dalam proses tumbuh kembang anak secara fisik maupun kecerdasan. Upaya dalam penurunan kasus stunting menjadi prioritas utama kota Probolinggo, dalam hal ini upaya perbaikan gizi yang dilakukan adalah melalui pemberian makanan tambahan (PMT) bagi balita dan ibu hamil. Tujuan kegiatan ini adalah untuk mendukung upaya perbaikan gizi guna mencegah gagal tumbuh kembang pada anak atau biasa dikenal dengan stunting. Sedangkan metode pelaksanaan kegiatan ini adalah menggunakan pelatihan pembuatan PMT yang kemudian disosialisasikan secara bertahap di desa Lemahkembar. Hasil pelaksanaan kegiatan ini adalah mengikuti Pendampingan posyandu melalui pembagian makanan tambahan yang bertujuan untuk meningkatkan gizi kepada ibu dan balita di Desa Lemahkembar serta menambah informasi mengenai bagaimana membuat inovasi makanan tambahan yang mengandung gizi tinggi seperti inovasi brownies kelor dan puding jagung. Selanjutnya, adalah Demonstrasi yang dilakukan bukan hanya mempresentasikan cara pembuatan melainkan juga memberikan pengetahuan materi tentang nutrisi yang terkandung didalam brownies daun kelor dan puding jagung.
Pelatihan Service Air Conditioner (AC) Bagi Pemuda HKBP Distrik XXIX Deli Serdang
Authors: Ronald Naibaho  , Napitupulu Abidan , Sinaga Ardian
Published: September 2024

HKBP Distrik XXIX Deli Serdang adalah salah satu denominasi gereja terbesar yang ada di Kabupaten Deli Serdang dengan jumlah 51 gereja. Jika rata-rata 1 buah gedung gereja HKBP menggunakan 8 buah AC maka  gedung gereja HKBP se Kabupaten Deli Serdang akan menggunakan 408 buah AC. Dengan demikian secara potensi jumlah akan membutuhkan pelaksana jasa service AC yang cukup, selain dapat dijadikan  peluang untuk membuka usaha jasa service AC. Dengan  melakukan pemberdayaan masyarakat kepada pemuda HKBP secara khusus bagi yang belum bekerja dan tidak berkesempatan kuliah diharapkan pemuda HKBP akan termotivasi. Melalui dialog, motivasi  dan diskusi terbuka terhadap permasalahan lapangan pekerjaan dan tantangan perekonomian Indonesia umumnya dan khususnya Sumatera Utara yang dihadapi saat ini dan dimasa yang akan datang sesuai dengan peluang pekejaan jasa service AC, Gereja HKBP dan Kabupaten Deli Serdang adalah potensi yang sangat besar. Dengan semakin tingginya suhu udara di Kabupaten Deli Sedang baik akibat pemanasan global dan kebutuhan jemaat yang semakin meningat untuk menggunakan AC di gedung gereja HKBP maka ketersediaan pelaku usaha service yang memberikan pelayanan terbaik sangat dibutuhkan. Kemampuan memberikan pelayanan jasa terbaik dan keterjangkauan biaya service menjadi salah satu peluang untuk membuka usaha. Selain itu tentunya dapat menghemat biaya maintenance jika pemuda gereja HKBP dimampukan menjadi sebuah tim pelaksana service AC Gedung Gereja HKBP. Adapun tujuan pengabdian masyarakat desa (PMD) ini adalah untuk memotivasi Pemuda Gereja HKBP se Deli Sedang mengetahui skill service AC dan  memahami peluang membuka usaha jasa service AC  yang akan semakin menjajnjikan sebagai sebuah usaha kecil dan menengah (UMK) yang modalnya tidak terlalu besar.  Dengan adanya dialog, memberikan motivasi dan diskusi maka pengetahuan Pemuda Gereja HKBP bertambah dan semakin termotivasi untuk menservice  AC gereja secara mandiri dan membuka usaha jasa service AC. Dengan metode pendekatan yang partisipatif dan kolaborasi beberapa pemuda ternyata memberikan dampak yang positif. Hal ini terlihat dari adanya respon dan komitmen untuk praktek langsung melakukan service AC di kampus ATDS.
Kesadaran Komunitas Lokal Untuk Pengembangan Pariwisata Inklusif di Desa Tegaren, Trenggalek
Authors: Nuryananda Praja Firdaus , Suksmawati Herlina , Rahmatin Leily Suci
Published: September 2024

Pada masa sekarang ini, penting kiranya untuk membangun kepariwisataan yang didasarkan pada kesadaran akan pentingnya inklusivitas. Artikel ini merupakan publikasi rangkuman kegiatan pengabdian kepada masyarakat di Desa Wisata Tegaren, Kab. Trenggalek, Jawa Timur. Tim pelaksana program mencoba untuk mengambil intisari dari rangkaian kegiatan pengabdian masyarakat yang telah dilakukan. Adapun intisari dari kegiatan tersebut terbagi menjadi dua, yakni 1) bahwa kesadaran inklusivitas di Tegaren telah muncul lama, yakni berbarengan dengan dirintisnya desa wisata, dan 2) adanya kesadaran akan inklusivitas tersebut akhirnya membuat kolaborasi dengan para stakeholders sangat terbantu dan dapat membantu Tegaren dalam merancang langkah konkret selanjutnya untuk mengembangkan pariwisata di desa, salah satunya melalui virtual tour. Tim pelaksana program menggunakan pendekatan partisipatoris dalam merancang, melaksanakan, serta melakukan evaluasi program pengabdian kepada masyarakat di Tegaren. Adapun pendekatan tersebut adalah participatory rural appraisal (PRA), asset-based community development (ABCD), collaborative partnership, dan community-based tourism (CBT). Dengan menggunakan banyak pendekatan partisipatoris, artinya tim pelaksana juga meletakkan peran penting pada komunitas atau masyarakat lokal di Tegaren. Peran yang semakin penting oleh komunitas atau masyarakat lokal ini yang kemudian secara simultan menumbuhkan kesadaran-kesadaran kolektif, seperti kesadaran akan inklusivitas.
Sosialisasi Peran Penting Generasi Z Menuju Indonesia Emas 2045 Bagi Siswa-siswi SMK Swasta Karya Jaya Tanjung Morawa
Authors: Panjaitan Joel , Pakpahan Arnold , Anwar Syahputra Syofyan , Sirait Regina , Fitriani Ayu , Simanullang Abdi Raih , Putri Femmy Armaya
Published: September 2024

Tujuan kegiatan ini adalah memberikan sosialisasi, memotivasi serta mengembangkan wawasan para Siswa-siswi SMK Swasta Karya Jaya Tanjung Morawa tentang peran penting generasi Z menuju Indonesia Emas. Dan diharapkan melalui kegiatan ini peserta mulai mempersiapkan diri dalam menyonsong Indonesia Emas 2045. Adapun kegiatan yang dilaksanakan adalah memberikan ceramah yang memuat langkah-langkah bagaimana mempersiapkan diri untuk menyonsong Indonesia Emas 2045. Dari hasil kegiatan pengabdian ini diketahui bahwa tingkat pemahaman peserta semakin meningkat. Hal ini dilihat dari hasil kusioner yang diberikan sebelum dan sesudah kegiatan dilaksanakan. Berdasarkan hasil kuisioner diperoleh bahwa pemahaman peserta tentang Bonus Demografi & Indonesia Emas 2045 mengalami peningkatan. Sebelum mengikuti kegiatan pelatihan nilai rata-rata adalah 40, namun setelah mengikuti kegiatan nilai rata-rata menjadi 80, maka persentase peningkatan sebesar 100%. Pemahaman peserta tentang Peran Generasi Z dalam menyonsong Indonesia Emas 2045 juga mengalami peningkatan. Sebelum mengikuti kegiatan penyuluhan dan pelatihan nilai rata-rata adalah 50, namun setelah mengikuti kegiatan nilai rata-rata menjadi 75, maka persentase peningkatan sebesar 50%. Pemahaman peserta tentang tentang langkah-langkah dan kesiapan dalam menyonsong Indonesia Emas 2045 juga mengalami peningkatan. Sebelum mengikuti kegiatan penyuluhan dan pelatihan nilai rata-rata adalah 50, namun setelah mengikuti kegiatan nilai rata-rata menjadi 90, maka persentase peningkatan sebesar 80%. Setelah pelaksanaan pelatihan dilakukan, terlihat peningkatan pengetahuan para peserta secara signifikan. Setelah pelaksanaan pelatihan dilakukan, terlihat peningkatan pengetahuan para peserta secara signifikan. Para peserta juga sangat antusias untuk mengikuti kegiatan ini. Hal ini dilihat dari banyaknya pertanyaan yang disampaikan oleh peserta kepada pemateri.
Cavernous Haemangioma- Uncommon Per rectal Bleeding
Authors: Rahman MM
Published: September 2024

Haemangiomas are uncommon in GIT, but cavernous haemangiomas are relatively common. Therefore this report is a case ofcavernous haemangioma in descending colon in a young male patient of 21 years present with colicky left upper abdominalpain and per rectal bleeding for 3 years. On clinical examination and imaging a pedunculated polypoid mass detected indescending colon. After laparotomy with left haemicolectomy a venous haemangioma found (confirmed by histopathologyexamination) surgical resection confirms this as benign tumor having good prognosis.
Childhood Obesity
Authors: Biswas B K
Published: September 2024

Paediatricians have been facing a very commoncomplaints: ‘‘Look doctor, my child is thin & has beenbecoming thinner day by day and not eating enough.”Although their kids achieved almost all the parameters ofnormal growth & development. Traditionally, a fat child isconsidered as an ‘‘attractive’’ child and is often referred toas an ‘‘healthy” child. However, the adverse effects andserious consequences of childhood obesity are now provenbeyond doubt. At least 30% of obesity begins at childhood.Conversely 50 to 80% of obese children, become obeseadults Many longitudinal studies have demonstrated1.convincingly, the higher risks of child onset obesity In2,3.the Harvard study, morbidity from cardiovascular disease,diabetes, obesity related cancers and arthritis was 50 to100% higher in obese individuals who were also obese aschildren and the cardiovascular mortality in suchindividuals was doubled.
Health Problems of the Elderly Attending Tertiary Level Hospital in Urban Area
Authors: Kabir S M H
Published: September 2024

Background: Health problems of the elderly are an emerging health burden through the world. Bangladesh is currently undergoing a demographic transition and the proportion of the population of 60 years and older is increasing rapidly. Health care providers and policy makers are highly concerned with this burning issue.

Objective: This study was conducted to explore the disease pattern among the elderly patients admitted in a tertiary care hospital in Dhaka city.

Methods: This cross sectional study was carried out from July 2015-June 2016 among the elderly patients above 60 years age admitted in the Combined Military Hospital (CMH), Dhaka. Total 152 respondents were selected by systematic random sampling technique. Data were collected by face-to-face interview with a semi-structured questionnaire and a checklist. Analysis of data was done by using "Statistical Package for Social Science” (SPSS, version 20.0) in the computer.

Results: Mean age of the respondents was 72.06±4.56 years and 90.8% of the respondents were male. Majority i.e 48 (31.6%) were in business group followed by 46 (30.3%) were in retired group and 14 (9.2 %) were house wife. Average monthly family income was Tk- 17927.63±7360.75 with the range of Tk- 6000- 35000. Regarding health problems, General weakness and Numbness of limbs 48 (31.57%), Musculo skeletal problems 25 (16.44%), Eye problems 18 (11.84%), GIT problems 15 (9.86%), Respiratory problems 12 (7.89%), Cardio vascular problems 20 (8.55%), ENT problems 15 (9.86%), Uro-genital problems 14 (9.21%) were found among the respondents. Mean hospital stay was 6.26±5.47 days and about 73% of the respondents were satisfied by hospital services.

Conclusion: The number of older people in the low-income countries is expanding rapidly. It also presents multifaceted health problems not commonly associated with low-income countries and thus creates unique challenges for the national health care service.
Multiple unilateral angiofibromas with a hypopigmented patch as a possible presentation of tuberous sclerosis complex (TSC)
Authors:   Dermatology Department, King Abdullah Medical City, Makkah, Saudi Arabia, Aboud Ahmad Al ,   Dermatology Department; King Faisal Hospital, Makkah, Saudi Arabia, Hakim Mohammad ,   OPD Department; King Faisal Hospital, Makkah, Saudi Arabia, Alheebi Ibrahim , Alotaibi Homaid , Bondogji Yusra Bondogji , Alsaadi Faris , Alghamdi Waleed , Saifeddin Ridha , Aldairi Abdulrahman Saleh , Alsaadi Ithar , Sabri Khalid , Alshamrani Ali , Alabsi Safa , Almalki Abdulrahman , Aboud Khalid Al
Published: September 2024

Tuberous sclerosis complex (TSC), known as Bourneville’s disease or epiloia, is an autosomal dominant inherited disorder that may affect numerous organ systems. TSC has many forms of clinical presentation. The cutaneous features of TSC include facial angiofibromas, shagreen patches, ash leaf hypopigmented macules, periungual fibromas, forehead fibrous plaques, confetti hypopigmentation, and poliosis. Rarely, the angiofibromas in TSC have a segmental distribution. It has been suggested that the segmental expression of tuberous sclerosis may result from a postzygotic mutation. The aim of this concise communication was to describe a patient with unilateral angiofibromas associated with a hypopigmented patch. No other features of TSC were present. We think this could have been a rare presentation of TSC. Key words: Angiofibroma, Hypopigmented patch, Loss of heterozygosity (LOH), Tuberous sclerosis complex (TSC)
Primary skeletal muscle anaplastic large cell lymphoma mimicking dermohypodermatitis
Authors:   Department of Dermatology, University Hospital Hassan II, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, Morocco, El-Ammari Sara , Elloudi Sara , Alaoui Imane Kacimi , Baybay Hanane , Soughi Meryem , Douhi Zakia , Mernissi Fatima Zahra , Alami Badre Eddine , Fadelallah Imane Fadelallah , Elousrouti Layla Tahiri ,   Department of Radiology, University Hospital Hassan II, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, Morocco,   Anatomical Pathology Laboratory, University Hospital Hassan II, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, Morocco
Published: September 2024

Anaplastic large cell lymphoma (ALCL) is a non-Hodgkin’s lymphoma (NHL) characterized by a strong expression of CD30. ALK-ALCL is a rare subtype that typically affects older adults and has a poor prognosis. It rarely arises in the skeletal muscle, particularly with primary involvement. Herein, we report the case of ALK-ALCL with primary involvement of the muscle. A 69-year-old male patient presented with fever, an erysipeloid placard in the axillary cavity, and the lateral and anterior chest wall initially treated with antibiotic therapy without improvement. A CT scan revealed a tumor proliferation in the pectoralis minor muscle, whose anatomopathological and immunohistochemical studies were in favor of ALCL. ALCL may mimic common dermatological conditions, including dermohypodermatitis. Therefore, in cases where symptoms do not resolve after conventional treatment, radiological examinations or even a biopsy should be considered to determine the cause of the symptoms and to initiate an appropriate treatment strategy. Key words: Anaplastic large cell lymphoma, Dermohypodermatitis, Muscle
Evaluation and management of cardiovascular disease and metabolic syndrome in pediatric-adolescent patients with psoriasis
Authors:   Department of Medicine, Richmond University Medical Center, Staten Island, NY 10310, US, Palmer Victoria ,   epartment of Dermatology and Dermatologic Surgery, Medical University of South Carolina, Charleston, SC 29425, USA,   Department of Pediatrics, Medical University of South Carolina, Charleston, SC 29425, USA, Valdebran Manuel
Published: September 2024

Psoriasis is strongly associated with cardiovascular disease (CVD) and metabolic syndrome, with pediatric and adolescent patients having an increased risk of each compared to the general population. This increased risk is based on shared underlying genetic and cytokine profiles, as well as similar environmental risks. Many screening guidelines do not address the development of CVD and metabolic syndrome in these predisposed patients, particularly in the adolescent population. These deficits are evidenced by the absence of validated 10-year CVD risk calculators for pediatric and adolescent patients with chronic inflammatory diseases, as well as insufficient screening guidelines for insulin resistance in patients with psoriasis. This manuscript aims to contribute literature regarding allopathic and lifestyle recommendations to the most recent 2017 consensus guideline for the pediatric/adolescent population with psoriasis. PubMed, COCHRANE Database of Systematic Reviews and Google Scholar were reviewed by a panel of clinicians with clinical and research expertise in psoriasis and pediatric subspecialties. The patient-centered Strength of Recommendation Taxonomy (SORT) method was utilized to grade the quality of the evidence available. Updates in management surrounding the role of lipoprotein(a) in lipid panels, recommendations for exercise and weight management to decrease insulin resistance, as well as guidance for heart rate and blood pressure management were discussed. Screening and managing pediatric and adolescent patients with psoriasis for the associated comorbidities of CVD and metabolic syndrome is imperative. Future high-quality studies are needed to strengthen the evidence provided throughout this review, which acts as a framework for clinical practice. Key words: Psoriasis, Comorbidity, Exercise, Treatment, Coronary heart disease