bluuebunny/crossref_metadata_2025 huggingface dataset:
Generalized Fractional Bézier Curve with Shape Parameters
Authors: Said Mad Zain Syed Ahmad Aidil Adha , Misro Md Yushalify , Miura Kenjiro T.
Published: September 2021

The construction of new basis functions for the Bézier or B-spline curve has been one of the most popular themes in recent studies in Computer Aided Geometric Design (CAGD). Implementing the new basis functions with shape parameters provides a different viewpoint on how new types of basis functions can develop complex curves and surfaces beyond restricted formulation. The wide selection of shape parameters allows more control over the shape of the curves and surfaces without altering their control points. However, interpolated parametric curves with higher degrees tend to overshoot in the process of curve fitting, making it difficult to control the optimal length of the curved trajectory. Thus, a new parameter needs to be created to overcome this constraint to produce free-form shapes of curves and surfaces while still preserving the basic properties of the Bézier curve. In this work, a general fractional Bézier curve with shape parameters and a fractional parameter is presented. Furthermore, parametric and geometric continuity between two generalized fractional Bézier curves is discussed in this paper, as well as demonstrating the effect of the fractional parameter of curves and surfaces. However, the conventional parametric and geometric continuity can only be applied to connect curves at the endpoints. Hence, a new type of continuity called fractional continuity is proposed to overcome this limitation. Thus, with the curve flexibility and adjustability provided by the generalized fractional Bézier curve, the construction of complex engineering curves and surfaces will be more efficient.
Expected Shortfall Reliability—Added Value of Traditional Statistics and Advanced Artificial Intelligence for Market Risk Measurement Purposes
Authors: Carrillo Menéndez Santiago , Hassani Bertrand Kian
Published: September 2021

The Fundamental Review of the Trading Book is a market risk measurement and management regulation recently issued by the Basel Committee. This reform, often referred to as “Basel IV”, intends to strengthen the financial system. The newest capital standard relies on the use of the Expected Shortfall. This risk measure requires to get sufficient information in the tails to ensure its reliability, as this one has to be alimented by a sufficient quantity of relevant data (above the 97.5 percentile in the case of the regulation or interest). In this paper, after discussing the relevant features of Expected Shortfall for risk measurement purposes, we present and compare several methods allowing to ensure the reliability of the risk measure by generating information in the tails. We discuss these approaches with respect to their relevance considering the underlying situation when it comes to available data, allowing practitioners to select the most appropriate approach. We apply traditional statistical methodologies, for instance distribution fitting, kernel density estimation, Gaussian mixtures and conditional fitting by Expectation-Maximisation as well as AI related strategies, for instance a Synthetic Minority Over-sampling Technique implemented in a regression environment and Generative Adversarial Nets.
Changes in the Molecular Characteristics of Bovine and Marine Collagen in the Presence of Proteolytic Enzymes as a Stage Used in Scaffold Formation
Authors: Egorikhina Marfa N. , Semenycheva Ludmila L. , Chasova Victoria O. , Bronnikova Irina I. , Rubtsova Yulia P. , Zakharychev Evgeniy A. , Aleynik Diana Ya.
Published: September 2021

Biopolymers, in particular collagen and fibrinogen, are the leading materials for use in tissue engineering. When developing technology for scaffold formation, it is important to understand the properties of the source materials as well as the mechanisms that determine the formation of the scaffold structures. Both factors influence the properties of scaffolds to a great extent. Our present work aimed to identify the features of the molecular characteristics of collagens of different species origin and the changes they undergo during the enzymatic hydrolysis used for the process of scaffold formation. For this study, we used the methods of gel-penetrating chromatography, dynamic light scattering, reading IR spectra, and scanning electron microscopy. It was found that cod collagen (CC) and bovine collagen (BC) have different initial molecular weight parameters, and that, during hydrolysis, the majority of either type of protein is hydrolyzed by the proteolytic enzymes within the first minute. The differently sourced collagen samples were also hydrolyzed with the formation of two low molecular fractions: Mw ~ 10 kDa and ~20 kDa. In the case of CC, the microstructure of the final scaffolds contained denser, closely spaced fibrillar areas, while the BC-sourced scaffolds had narrow, short fibrils composed of unbound fibers of hydrolyzed collagen in their structure.
Prospective Comparison of 24-Hour Urine Creatinine Clearance with Estimated Glomerular Filtration Rates in Chronic Renal Disease Patients of African Descent
Authors: Tapper Marlene , McGrowder Donovan A. , Dilworth Lowell , Soyibo Adedamola
Published: September 2021

Background: The 24-hour (24-h) creatinine clearance (CrCl) is the most common method for measuring GFR in clinical laboratories. However, the limitations of CrCl have resulted in the widespread acceptance of mathematically derived estimated glomerular filtration rate (eGFR) using Cockcroft-Gault (CG), Modification of Diet in Renal Disease (MDRD) and the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations in predicting eGFR. The aim of the study was to compare 24-h CrCl with eGFR derived from these formulae and to identify which could be the best alternative. Method: A prospective study was conducted involving 140 CKD patients. Creatinine and cystatin C concentrations were determined using the cobas 6000 analyzer. The eGFR was calculated using the CG formula, 4-variable MDRD and CKD-EPI equations, and Bland-Alman plots bias was determined. Results: The CG and MDRD formulas had mean eGFR values similar to CrCl and correlation coefficients (r) were highest for CG (0.906) and lowest for MDRD (0.799). The CG equation was in agreement with 24-h CrCl in all but stage V CKD while the MDRD equation compared well in all except Stage IV CKD. The CG equation was positively biased (0.9857) while the MDRD had a negative bias (−0.05). Conclusion: The Cockcroft-Gault formula provides a more accurate assessment of GFR than 24-h CrCl and would be recommended as a substitute to provide the best estimate of GFR in our population.
Modeling and Optimization of Gas Sparging-Assisted Bacterial Cultivation Broth Microfiltration by Response Surface Methodology and Genetic Algorithm
Authors: Jokić Aleksandar , Pajčin Ivana , Lukić Nataša , Vlajkov Vanja , Kiralj Arpad , Dmitrović Selena , Grahovac Jovana
Published: September 2021

Production of highly efficient biomass-based microbial biopesticides significantly depends on downstream processing in terms of obtaining as high concentration of viable cells as possible. Microfiltration is one of the recommended operations for microbial biomass separation, but its main limitation is permeate flux decrease due to the membrane fouling. The effect of air sparging as a hydrodynamic technique for improvement of permeate flux during microfiltration of Bacillus velezensis cultivation broth was investigated. Modeling of the microfiltration was performed using the response surface methodology, while desirability function approach and genetic algorithm were applied for optimization, i.e., maximization of permeate flux and minimization of specific energy consumption. The results have revealed antagonistic relationship between the investigated dependent variables. The optimized values of superficial feed velocity and transmembrane pressure were close to the mean values of the investigated value ranges (0.68 bar and 0.96 m/s, respectively), while the optimized value of superficial air velocity had a more narrow distribution around 0.25 m/s. The results of this study have revealed a significant improvement of microfiltration performance by applying air sparging, thus this flux improvement method should be further investigated in downstream processing of different bacterial cultivation broths.
Infrasonic Nanocrystal Formation in Amorphous NiTi Film: Physical Mechanism, Reasons and Conditions
Authors: Slyadnikov Evgeny E.
Published: September 2021

The physical mechanism, reasons and conditions of nanocrystal formation in an amorphous NiTi metal film, stimulated by infrasonic action, are formulated. Nanostructural elements of an amorphous medium (relaxation centers) containing disordered nanoregions with two-level systems are considered to be responsible for this process. When exposed to infrasound, a large number of two-level systems are excited, significantly contributing to inelastic deformation and the formation of nanocrystals. The physical mechanism of the nanocrystallization of metallic glass under mechanical action includes both local thermal fluctuations and the additional quantum tunneling of atoms stimulated by shear deformation. A crystalline nanocluster appears as a result of local atomic rearrangement growing increasingly exposed to infrasound. It is possibly unstable in the absence of infrasound. When the radius of the nanocluster reaches a critical value, a potential well appears, in which a conducting electron is localized to form a phason (stable nanocrystal). Estimated values of the phason’s radius and the depth of the nanometer potential well is about 0.5 nm and 1 eV, respectively. It forms a condition of stable phason formation. The occurrence of the instability of the amorphous state and following transformation to the nanostructured state is based on the accumulation of the potential energy of inelastic deformation to a critical value equal to the latent heat of the transformation of the amorphous state into the nanostructured state.
Review of Powder Bed Fusion Additive Manufacturing for Metals
Authors: Ladani Leila , Sadeghilaridjani Maryam
Published: September 2021

Additive manufacturing (AM) as a disruptive technology has received much attention in recent years. In practice, however, much effort is focused on the AM of polymers. It is comparatively more expensive and more challenging to additively manufacture metallic parts due to their high temperature, the cost of producing powders, and capital outlays for metal additive manufacturing equipment. The main technology currently used by numerous companies in the aerospace and biomedical sectors to fabricate metallic parts is powder bed technology, in which either electron or laser beams are used to melt and fuse the powder particles line by line to make a three-dimensional part. Since this technology is new and also sought by manufacturers, many scientific questions have arisen that need to be answered. This manuscript gives an introduction to the technology and common materials and applications. Furthermore, the microstructure and quality of parts made using powder bed technology for several materials that are commonly fabricated using this technology are reviewed and the effects of several process parameters investigated in the literature are examined. New advances in fabricating highly conductive metals such as copper and aluminum are discussed and potential for future improvements is explored.
Study of Coatings Formed on Zirconium Alloy by Plasma Electrolytic Oxidation in Electrolyte with Submicron Yttria Powder Additives
Authors: Savushkina Svetlana , Gerasimov Mikhail , Apelfeld Andrey , Suminov Igor
Published: September 2021

Coatings with thickness 40 to 150 μm were formed by plasma electrolytic oxidation (PEO) on the zirconium alloy Zr-1Nb (Zr-1% Nb) in the slurry electrolyte containing 9 g/L Na2SiO3 5H2O, 5 g/L Na(PH2O2) and 6 g/L submicron Y2O3 yttria powder during 60 min under the AC electrical mode at current densities 20; 30 and 40 A/dm2. The surface morphology, structure, composition, and corrosion-protective ability of the formed coatings have been analyzed. At PEO current density 30 A/dm2, a predominantly tetragonal phase of zirconia was formed in coatings. Increasing the PEO current density up to 40 A/dm2 promoted the formation of the coating surface layer containing submicron yttria particles. Electrochemical polarization studies in 0.5% LiOH solution showed that PEO coatings demonstrated high corrosion-protective ability. The dependence of the polarization currents on the PEO current density was found to be inconsequential.
Will Casuarina glauca Stress Resilience Be Maintained in the Face of Climate Change?
Authors: Jorge Tiago F. , Ramalho José C. , Alseekh Saleh , Pais Isabel P. , Leitão António E. , Rodrigues Ana P. , Scotti-Campos Paula , Ribeiro-Barros Ana I. , Fernie Alisdair R. , António Carla
Published: September 2021

Actinorhizal plants have been regarded as promising species in the current climate change context due to their high tolerance to a multitude of abiotic stresses. While combined salt-heat stress effects have been studied in crop species, their impact on the model actinorhizal plant, Casuarina glauca, has not yet been fully addressed. The effect of single salt (400 mM NaCl) and heat (control at 26/22 °C, supra optimal temperatures at 35/22 °C and 45/22 °C day/night) conditions on C. glauca branchlets was characterised at the physiological level, and stress-induced metabolite changes were characterised by mass spectrometry-based metabolomics. C. glauca could withstand single salt and heat conditions. However, the harshest stress condition (400 mM NaCl, 45 °C) revealed photosynthetic impairments due to mesophyll and membrane permeability limitations as well as major stress-specific differential responses in C and N metabolism. The increased activity of enzymatic ROS scavengers was, however, revealed to be sufficient to control the plant oxidative status. Although C. glauca could tolerate single salt and heat stresses, their negative interaction enhanced the effects of salt stress. Results demonstrated that C. glauca responses to combined salt-heat stress could be explained as a sum of the responses from each single applied stress.
Effect of Intrapartum Antibiotics Prophylaxis on the Bifidobacterial Establishment within the Neonatal Gut
Authors: Saturio Silvia , Suárez Marta , Mancabelli Leonardo , Fernández Nuria , Mantecón Laura , de los Reyes-Gavilán Clara G. , Ventura Marco , Gueimonde Miguel , Arboleya Silvia , Solís Gonzalo
Published: September 2021

Antibiotics are important disruptors of the intestinal microbiota establishment, linked to immune and metabolic alterations. The intrapartum antibiotics prophylaxis (IAP) is a common clinical practice that is present in more than 30% of labours, and is known to negatively affect the gut microbiota composition. However, little is known about how it affects to Bifidobacterium (sub)species level, which is one of the most important intestinal microbial genera early in life. This study presents qualitative and quantitative analyses of the bifidobacterial (sub)species populations in faecal samples, collected at 2, 10, 30 and 90 days of life, from 43 healthy full-term babies, sixteen of them delivered after IAP use. This study uses both 16S rRNA–23S rRNA internal transcribed spacer (ITS) region sequencing and q-PCR techniques for the analyses of the relative proportions and absolute levels, respectively, of the bifidobacterial populations. Our results show that the bifidobacterial populations establishment is affected by the IAP at both quantitative and qualitative levels. This practice can promote higher bifidobacterial diversity and several changes at a compositional level. This study underlines specific targets for developing gut microbiota-based products for favouring a proper bifidobacterial microbiota development when IAP is required.
ASTER-Based Remote Sensing Image Analysis for Prospection Criteria of Podiform Chromite at the Khoy Ophiolite (NW Iran)
Authors: Mehdikhani Behnam , Imamalipour Ali
Published: September 2021

A single chromite deposit occurrence is found in the serpentinized harzburgite unit of the Khoy ophiolite complex in northwest Iran, which is surrounded by dunite envelopes. This area has mountainous features and extremely rugged topography with difficult access, so prospecting for chromite deposits by conventional geological mapping is challenging. Therefore, using remote sensing techniques is very useful and effective, in terms of saving costs and time, to determine the chromite-bearing zones. This study evaluated the discrimination of chromite-bearing mineralized zones within the Khoy ophiolite complex by analyzing the capabilities of ASTER satellite data. Spectral transformation methods such as optimum index factor (OIF), band ratio (BR), spectral angle mapper (SAM), and principal component analysis (PCA) were applied on the ASTER bands for lithological mapping. Many chromitite lenses are scattered in this ophiolite, but only a few have been explored. ASTER bands contain improved spectral characteristics and higher spatial resolution for detecting serpentinized dunite in ophiolitic complexes. In this study, after the correction of ASTER data, many conventional techniques were used. A specialized optimum index factor RGB (8, 6, 3) was developed using ASTER bands to differentiate lithological units. The color composition of band ratios such as RGB ((4 + 2)/3, (7 + 5)/6, (9 + 7)/8), (4/1, 4/7, 4/5), and (4/3 × 2/3, 3/4, 4/7) produced the best results. The integration of information extracted from the image processing algorithms used in this study mapped most of the lithological units of the Khoy ophiolitic complex and new prospecting targets for chromite exploration were determined. Furthermore, the results were verified by comprehensive fieldwork and previous studies in the study area. The results of this study indicate that the integration of information extracted from the image processing algorithms could be a broadly applicable tool for chromite prospecting and lithological mapping in mountainous and inaccessible regions such as Iranian ophiolitic zones.
Influence of In Vitro Human Digestion Simulation on the Phenolics Contents and Biological Activities of the Aqueous Extracts from Turkish Cistus Species
Authors: İnan Yiğit , Akyüz Selin , Kurt-Celep Inci , Celep Engin , Yesilada Erdem
Published: September 2021

Oxidative stress is one of the significant precursors of various metabolic diseases such as diabetes, Parkinson’s disease, cardiovascular diseases, cancer, etc. Various scientific reports have indicated that secondary plant metabolites play an important role in preventing oxidative stress and its harmful effects. In this respect, this study was planned to investigate the phenolic profile and antioxidant and antidiabetic potentials of the aqueous extracts from Turkish Cistus species by employing in vitro methods. In vitro digestion simulation procedure was applied to all extracts to estimate the bioavailability of their phenolic contents. Total phenolic, flavonoid, phenolic acid and proanthocyanidin contents were determined for all phases of digestion. In addition, changes in the quantity of the assigned marker flavonoids (tiliroside, hyperoside and quercitrin) were monitored by High-Performance Thin Layer Chromatography (HPTLC) analysis. The antioxidant activity potentials of the extracts were studied by various methods to reveal their detailed activity profiles. On the other hand, in vitro α-amylase and α-glucosidase enzymes and advanced-glycation end product (AGE) inhibitory activities of the extracts were determined to evaluate the antidiabetic potentials of extracts. The results showed that aqueous extracts obtained from the aerial parts of Turkish Cistus species have rich phenolic contents and potential antioxidant and antidiabetic activities; however, their bioactivity profiles and marker flavonoid concentrations might significantly be affected by human digestion. The results exhibited that total phenolic contents, antioxidant activities and diabetes-related enzyme inhibitions of the bioavailable samples were lower than non-digested samples in all extracts.
Surface-Functionalized Separator for Stable and Reliable Lithium Metal Batteries: A Review
Authors: Kim Patrick Joohyun
Published: September 2021

Metallic Li has caught the attention of researchers studying future anodes for next-generation batteries, owing to its attractive properties: high theoretical capacity, highly negative standard potential, and very low density. However, inevitable issues, such as inhomogeneous Li deposition/dissolution and poor Coulombic efficiency, hinder the pragmatic use of Li anodes for commercial rechargeable batteries. As one of viable strategies, the surface functionalization of polymer separators has recently drawn significant attention from industries and academics to tackle the inherent issues of metallic Li anodes. In this article, separator-coating materials are classified into five or six categories to give a general guideline for fabricating functional separators compatible with post-lithium-ion batteries. The overall research trends and outlook for surface-functionalized separators are reviewed.
Preparation of TiSi2 Powders with Enhanced Lithium-Ion Storage via Chemical Oven Self-Propagating High-Temperature Synthesis
Authors: Xu Jianguang , Jin Menglan , Shi Xinlu , Li Qiuyu , Gan Chengqiang , Yao Wei
Published: September 2021

Although silicon has highest specific capacity as anode for lithium-ion battery (LIB), its large volume change during the charge/discharge process becomes a great inevitable hindrance before commercialization. Metal silicides may be an alternative choice because they have the ability to accommodate the volume change by dispersing Si in the metal matrix as well as very good electrical conductivity. Herein we report on the suitability of lithium-ion uptake in C54 TiSi2 prepared by the “chemical oven” self-propagating high-temperature synthesis from the element reactants, which was known as an inactive metal silicide in lithium-ion storage previously. After being wrapped by graphene, the agglomeration of TiSi2 particles has been efficiently prevented, resulting in an enhanced lithium-ion storage performance when using as an anode for LIB. The as-received TiSi2/RGO hybrid exhibits considerable activities in the reversible lithiation and delithiation process, showing a high reversible capacity of 358 mAh/g at a current density of 50 mA/g. Specially, both TiSi2 and TiSi2/RGO electrodes show a remarkable enhanced electrochemical performance along with the cycle number, indicating the promising potential in lithium-ion storage of this silicide. Ex-situ XRD during charge/discharge process reveals alloying reaction may contribute to the capacity of TiSi2. This work suggests that TiSi2 and other inactive transition metal silicides are potential promising anode materials for Li-ion battery and capacitor.
A Single Crystal Hybrid Ligand Framework of Copper(II) with Stable Intrinsic Blue-Light Luminescence in Aqueous Solution
Authors: Charoensuk Suwitra , Tan Jing , Sain Mohini , Manuspiya Hathaikarn
Published: September 2021

Single-crystal solid–liquid dual-phase hybrid organic–inorganic ligand frameworks with reversible sensing response facilitated by external stimuli have received significant attention in recent years. This report presents a significant leap in designing electronic structures that display reversible dual-phase photoluminescence properties from single-crystal hybrid ligand frameworks. Three-dimensional Cu(C3N2H4)4Cl2 complex frameworks were formed through the intermolecular hydrogen bonding and π⋯π stacking supramolecular interactions. The absorption band peaks at 627 nm were assigned to d–d transition showing 10Dq = 15,949 cm−1 and crystal field stabilization energy (CFSE) = 0.6 × 10Dq = 114.4 kJmol−1, while the ligand-to-metal charge transfer (LMCT) of complexes was displayed at 292 nm. The intense luminescence band results from LMCT present at 397 nm. Considering its structure, air stability, framework forming and stable luminescence in aqueous solution, the Cu(C3N2H4)4Cl2 complex shows potential for luminescence Cu-based sensors using emission intensity to detect heavy metal ion species.
Bimetallic Nanowires on Laser-Patterned PEN as Promising Biomaterials
Authors: Pryjmaková Jana , Kaimlová Markéta , Vokatá Barbora , Hubáček Tomáš , Slepička Petr , Švorčík Václav , Siegel Jakub
Published: September 2021

As inflammation frequently occurs after the implantation of a medical device, biocompatible, antibacterial materials must be used. Polymer–metal nanocomposites are promising materials. Here we prepared enhanced polyethylene naphthalate (PEN) using surface modification techniques and investigated its suitability for biomedical applications. The PEN was modified by a KrF laser forming periodic ripple patterns with specific surface characteristics. Next, Au/Ag nanowires were deposited onto the patterned PEN using vacuum evaporation. Atomic force microscopy confirmed that the surface morphology of the modified PEN changed accordingly with the incidence angle of the laser beam. Energy-dispersive X-ray spectroscopy showed that the distribution of the selected metals was dependent on the evaporation technique. Our bimetallic nanowires appear to be promising antibacterial agents due to the presence of antibacterial noble metals. The antibacterial effect of the prepared Au/Ag nanowires against E. coli and S. epidermidis was demonstrated using 24 h incubation with a drop plate test. Moreover, a WST-1 cytotoxicity test that was performed to determine the toxicity of the nanowires showed that the materials could be considered non-toxic. Collectively, these results suggest that prepared Au/Ag nanostructures are effective, biocompatible surface coatings for use in medical devices.
Erratum: Rémy et al. Modelling the Impact of Chronic Cigarette Smoke Exposure in Obese Mice: Metabolic, Pulmonary, Intestinal, and Cardiac Issues. Nutrients 2020, 12, 827
Authors: Rémy Gaëlle , Dubois-Deruy Emilie , Alard Jeanne , Kervoaze Gwenola , Chwastyniak Maggy , Baron Morgane , Beury Delphine , Siegwald Léa , Caboche Ségolène , Hot David , Gosset Philippe , Grangette Corinne , Pinet Florence , Wolowczuk Isabelle , Pichavant Muriel
Published: September 2021

The authors have requested that the following changes be made to their paper [...]
Effect of Advanced Glycation End-Products and Excessive Calorie Intake on Diet-Induced Chronic Low-Grade Inflammation Biomarkers in Murine Models
Authors: Nogueira Silva Lima Matheus Thomaz , Howsam Michael , Anton Pauline M. , Delayre-Orthez Carine , Tessier Frédéric J.
Published: September 2021

Chronic Low-Grade Inflammation (CLGI) is a non-overt inflammatory state characterized by a continuous activation of inflammation mediators associated with metabolic diseases. It has been linked to the overconsumption of Advanced Glycation End-Products (AGEs), and/or macronutrients which lead to an increase in local and systemic pro-inflammatory biomarkers in humans and animal models. This review provides a summary of research into biomarkers of diet-induced CLGI in murine models, with a focus on AGEs and obesogenic diets, and presents the physiological effects described in the literature. Diet-induced CLGI is associated with metabolic endotoxemia, and/or gut microbiota remodeling in rodents. The mechanisms identified so far are centered on pro-inflammatory axes such as the interaction between AGEs and their main receptor AGEs (RAGE) or increased levels of lipopolysaccharide. The use of murine models has helped to elucidate the local and systemic expression of CLGI mediators. These models have enabled significant advances in identification of diet-induced CLGI biomarkers and resultant physiological effects. Some limitations on the translational (murine → humans) use of biomarkers may arise, but murine models have greatly facilitated the testing of specific dietary components. However, there remains a lack of information at the whole-organism level of organization, as well as a lack of consensus on the best biomarker for use in CLGI studies and recommendations as to future research conclude this review.
Dietary-Induced Ketogenesis: Adults Are Not Children
Authors: Porper Keren , Zach Leor , Shpatz Yael , Ben-Zeev Bruria , Tzadok Michal , Jan Elisheva , Talianski Alisa , Champ Colin E. , Symon Zvi , Anikster Yair , Lawrence Yaacov R.
Published: September 2021

There is increasing interest in the use of a ketogenic diet for various adult disorders; however, the ability of adults to generate ketones is unknown. Our goal was to challenge the hypothesis that there would be no difference between adults and children regarding their ability to enter ketosis. Methods: Two populations were studied, both treated with identical very low-carbohydrate high-fat diets: a retrospective series of children with epilepsy or/and metabolic disorders (2009–2016) and a prospective clinical trial of adults with glioblastoma. Dietary intake was assessed based upon written food diaries and 24-h dietary recall. Ketogenic ratio was calculated according to [grams of fat consumed]/[grams of carbohydrate and protein consumed]. Ketone levels (β-hydroxybutyrate) were measured in blood and/or urine. Results: A total of 168 encounters amongst 28 individuals were analyzed. Amongst both children and adults, ketone levels correlated with nutritional ketogenic ratio; however, the absolute ketone levels in adults were approximately one quarter of those seen in children. This difference was highly significant in a multivariate linear regression model, p < 0.0001. Conclusions: For diets with comparable ketogenic ratios, adults have lower blood ketone levels than children; consequently, high levels of nutritional ketosis are unobtainable in adults.
Enhancing Bioavailability of Nutraceutically Used Resveratrol and Other Stilbenoids
Authors: Vesely Ondrej , Baldovska Simona , Kolesarova Adriana
Published: September 2021

Stilbenoids are interesting natural compounds with pleiotropic in vitro and in vivo activity. Their well-documented biological properties include anti-inflammatory effects, anticancer effects, effects on longevity, and many others. Therefore, they are nowadays commonly found in foods and dietary supplements, and used as a part of treatment strategy in various types of diseases. Bioactivity of stilbenoids strongly depends on different types of factors such as dosage, food composition, and synergistic effects with other plant secondary metabolites such as polyphenols or vitamins. In this review, we summarize the existing in vitro, in vivo, and clinical data from published studies addressing the optimization of bioavailability of stilbenoids. Stilbenoids face low bioavailability due to their chemical structure. This can be improved by the use of novel drug delivery systems or enhancers, which are discussed in this review. Current in vitro and in vivo evidence suggests that both approaches are very promising and increase the absorption of the original substance by several times. However, data from more clinical trials are required.
Delineating the Role of Aedes aegypti ABC Transporter Gene Family during Mosquito Development and Arboviral Infection via Transcriptome Analyses
Authors: Kumar Vikas , Garg Shilpi , Gupta Lalita , Gupta Kuldeep , Diagne Cheikh Tidiane , Missé Dorothée , Pompon Julien , Kumar Sanjeev , Saxena Vishal
Published: September 2021

Aedes aegypti acts as a vector for several arboviral diseases that impose a major socio-economic burden. Moreover, the absence of a vaccine against these diseases and drug resistance in mosquitoes necessitates the development of new control strategies for vector-borne diseases. ABC transporters that play a vital role in immunity and other cellular processes in different organisms may act as non-canonical immune molecules against arboviruses, however, their role in mosquito immunity remains unexplored. This study comprehensively analyzed various genetic features of putative ABC transporters and classified them into A-H subfamilies based on their evolutionary relationships. Existing RNA-sequencing data analysis indicated higher expression of cytosolic ABC transporter genes (E & F Subfamily) throughout the mosquito development, while members of other subfamilies exhibited tissue and time-specific expression. Furthermore, comparative gene expression analysis from the microarray dataset of mosquito infected with dengue, yellow fever and West Nile viruses revealed 31 commonly expressed ABC transporters suggesting a potentially conserved transcriptomic signature of arboviral infection. Among these, only a few transporters of ABCA, ABCC and ABCF subfamily were upregulated, while most were downregulated. This indicates the possible involvement of ABC transporters in mosquito immunity.
Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples
Authors: Weinreich Felix , Hahn Andreas , Eberhardt Kirsten Alexandra , Feldt Torsten , Sarfo Fred Stephen , Di Cristanziano Veronica , Frickmann Hagen , Loderstädt Ulrike
Published: September 2021

As qualified microscopy of enteric parasitoses as defined by high diagnostic accuracy is difficult to maintain in non-endemic areas due to scarce opportunities for practicing with positive sample materials, molecular diagnostic options provide less investigator-dependent alternatives. Here, we compared three molecular targets for the real-time PCR-based detection of Cryptosporidium spp. From a population of 1000 individuals comprising both Ghanaian HIV (human immunodeficiency virus) patients and military returnees after deployment in the tropics, stool samples were assessed for Cryptosporidium spp. by real-time PCR targeting the small subunit ribosomal RNA (SSU rRNA) gene, the Cryptosporidium oocyst wall (COWP) gene, and the DnaJ-like protein gene (DnaJ), respectively. In declining order, sensitivity of 100% for the SSU rRNA gene PCR, 90.0% for the COWP PCR and 88.8% for the DnaJ PCR, respectively, as well as specificity of 99.6% for the COWP PCR and 96.9% for both the SSU rRNA gene PCR and the DnaJ PCR, respectively, were recorded. Substantial agreement (kappa value 0.663) between the three assays was observed. Further, an accuracy-adjusted Cryptosporidium spp. prevalence of 6.0% was calculated for the study population. In conclusion, none of the assessed real-time PCR assays were associated with perfect test accuracy. However, a combination of highly sensitive SSU rRNA gene PCR for screening purposes and more specific COWP PCR for confirmatory testing should allow reliable diagnosis of Cryptosporidium spp. in stool samples even in low prevalence settings.
Accuracy and Impact on Patient Management of New Tools for Diagnosis of Sepsis: Experience with the T2 Magnetic Resonance Bacteria Panel
Authors: Paggi Riccardo , Cenci Elio , De Socio Giuseppe , Belati Alessandra , Marini Daniele , Gili Alessio , Camilloni Barbara , Mencacci Antonella
Published: September 2021

The rapid and accurate identification of pathogens responsible for sepsis is essential for prompt and effective antimicrobial therapy. Molecular technologies have been developed to detect the most common causative agents, with high sensitivity and short time to result (TTR). T2 Bacteria Panel (T2), based on a combination of PCR and T2 magnetic resonance, can identify directly in blood samples Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium, and Acinetobacter baumannii pathogens. This study evaluates the role of T2 in the diagnosis of sepsis and its impact on patient management, specifically in terms of TTR and the switch from empirical to directed therapy, comparing results of blood culture (BC) and T2 assay in 82 patients with sepsis. T2 significantly improved the detection of the causative agents of sepsis. For pathogens included in the panel, T2 sensitivity was 100% (95% CI 86.3–100.0), significantly higher than that of BC (54.8%, 95% CI 36.0–72.7). The TTR (median, IQR) of positive T2 (3.66 h, 3.59–4.31) was significantly shorter than that of the positive BC (37.58 h, 20.10–47.32). A significant reduction in the duration of empiric therapy and an increase in the percentage of patients with switched therapy was observed in patients with a positive T2 result. In conclusion, T2 can shorten and improve the etiological diagnosis of sepsis with a positive impact on patient management.
The Basis of Tolerance Mechanism to Metsulfuron-Methyl in Roegneria kamoji (Triticeae: Poaceae)
Authors: Tang Wei , Liu Shengnan , Yu Xiaoyue , Yang Yongjie , Zhou Xiaogang , Lu Yongliang
Published: September 2021

Roegneria kamoji, a perennial monocot weed that belongs to the tribe Triticeae (family: Poaceae), is an emerging problematic weed in winter wheat (Triticum aestivum) fields in China. We have previously confirmed four R. kamoji populations tolerant to acetyl-CoA carboxylase (ACCase) inhibitors, and failed control of these populations by metsulfuron-methyl was observed. The objective of this study was to characterize the level of tolerance to metsulfuron-methyl, the basis of tolerance mechanism, and cross-tolerance to acetolactate synthase (ALS) inhibitors in R. kamoji. A whole-plant dose–response assay showed that plants of all R. kamoji populations (both from wheat fields and uncultivated areas) exhibited high tolerance to metsulfuron-methyl, based on their 100% survival at 6-fold recommended field dose (RFD) and ED50 values >6.84-fold RFD, no susceptible population was found. Gene sequencing indicated that no reported amino acid substitutions associated with resistance to ALS inhibitor were found in the ALS gene among the R. kamoji populations. Pretreatment with the known cytochrome P450 monooxygenases (CytP450) inhibitor malathion reduced the ED50 values of metsulfuron-methyl in two R. kamoji populations. These populations also exhibited cross-tolerance to RFD of mesosulfuron-methyl and bispyribac-sodium. The activities of glutathione-S-transferase (GST) and CytP450 could be induced by metsulfuron-methyl in R. kamoji, which is similar to the known tolerant crop wheat. This is the first report elucidating metsulfuron-methyl tolerance in R. kamoji. The reversal of tolerance by malathion and the GST and/or CytP450 enhanced herbicide metabolism suggests that non-target-site mechanisms confer tolerance to metsulfuron-methyl in R. kamoji.
Morpho-Physiological Classification of Italian Tomato Cultivars (Solanum lycopersicum L.) According to Drought Tolerance during Vegetative and Reproductive Growth
Authors: Conti Veronica , Romi Marco , Parri Sara , Aloisi Iris , Marino Giovanni , Cai Giampiero , Cantini Claudio
Published: September 2021

Irrigation is fundamental for agriculture but, as climate change becomes more persistent, there is a need to conserve water and use it more efficiently. It is therefore crucial to identify cultivars that can tolerate drought. For economically relevant crops, such as tomatoes, this purpose takes on an even more incisive role and local agrobiodiversity is a large genetic reservoir of promising cultivars. In this study, nine local Italian cultivars of tomatoes plus four widely used commercial cultivars were considered. These experienced about 20 d of drought, either at vegetative or reproductive phase. Various physio-morphological parameters were monitored, such as stomatal conductance (gs), photosynthesis (A), water use efficiency (WUE), growth (GI) and soil water content (SWC). The different responses and behaviors allowed to divide the cultivars into three groups: tolerant, susceptible, and intermediate. The classification was also confirmed by a principal component analysis (PCA). The study, in addition to deepening the knowledge of local Italian tomato cultivars, reveals how some cultivars perform better under stress condition than commercial ones. Moreover, the different behavior depends on the genotype and on the growth phase of plants. In fact, the Perina cultivar is the most tolerant during vegetative growth while the Quarantino cultivar is mostly tolerant at reproductive stage. The results suggest that selection of cultivars could lead to a more sustainable agriculture and less wasteful irrigation plans.
Supramolecular Self-Healing Sensor Fiber Composites for Damage Detection in Piezoresistive Electronic Skin for Soft Robots
Authors: Georgopoulou Antonia , Bosman Anton W. , Brancart Joost , Vanderborght Bram , Clemens Frank
Published: September 2021

Self-healing materials can prolong the lifetime of structures and products by enabling the repairing of damage. However, detecting the damage and the progress of the healing process remains an important issue. In this study, self-healing, piezoresistive strain sensor fibers (ShSFs) are used for detecting strain deformation and damage in a self-healing elastomeric matrix. The ShSFs were embedded in the self-healing matrix for the development of self-healing sensor fiber composites (ShSFC) with elongation at break values of up to 100%. A quadruple hydrogen-bonded supramolecular elastomer was used as a matrix material. The ShSFCs exhibited a reproducible and monotonic response. The ShSFCs were investigated for use as sensorized electronic skin on 3D-printed soft robotic modules, such as bending actuators. Depending on the bending actuator module, the electronic skin was loaded under either compression (pneumatic-based module) or tension (tendon-based module). In both configurations, the ShSFs could be successfully used as deformation sensors, and in addition, detect the presence of damage based on the sensor signal drift. The sensor under tension showed better recovery of the signal after healing, and smaller signal relaxation. Even with the complete severing of the fiber, the piezoresistive properties returned after the healing, but in that case, thermal heat treatment was required. With their resilient response and self-healing properties, the supramolecular fiber composites can be used for the next generation of soft robotic modules.
Gas to Liquids Techno-Economics of Associated Natural Gas, Bio Gas, and Landfill Gas
Authors: Galli Federico , Lai Jun-Jie , De Tommaso Jacopo , Pauletto Gianluca , Patience Gregory S.
Published: September 2021

Methane is the second highest contributor to the greenhouse effect. Its global warming potential is 37 times that of CO2. Flaring-associated natural gas from remote oil reservoirs is currently the only economical alternative. Gas-to-liquid (GtL) technologies first convert natural gas into syngas, then it into liquids such as methanol, Fischer–Tropsch fuels or dimethyl ether. However, studies on the influence of feedstock composition are sparse, which also poses technical design challenges. Here, we examine the techno-economic analysis of a micro-refinery unit (MRU) that partially oxidizes methane-rich feedstocks and polymerizes the syngas formed via Fischer–Tropsch reaction. We consider three methane-containing waste gases: natural gas, biogas, and landfill gas. The FT fuel selling price is critical for the economy of the unit. A Monte Carlo simulation assesses the influence of the composition on the final product quantity as well as on the capital and operative expenses. The Aspen Plus simulation and Python calculate the net present value and payback time of the MRU for different price scenarios. The CO2 content in biogas and landfill gas limit the CO/H2 ratio to 1.3 and 0.9, respectively, which increases the olefins content of the final product. Compressors are the main source of capital cost while the labor cost represents 20–25% of the variable cost. An analysis of the impact of the plant dimension demonstrated that the higher number represents a favorable business model for this unit. A minimal production of 7,300,000 kg y−1 is required for MRU to have a positive net present value after 10 years when natural gas is the feedstock.
Development, Optimization and Validation of a Sustainable and Quantifiable Methodology for the Determination of 2,4,6-Trichloroanisole, 2,3,4,6-Tetrachloroanisole, 2,4,6-Tribromoanisole, Pentachloroanisole, 2-Methylisoborneole and Geosmin in Air
Authors: Jové Patricia , Vives-Mestres Marina , Nadal Raquel De , Verdum Maria
Published: September 2021

Compounds 2,4,6-trichloroanisole (TCA), 2,3,4,6-tetrachloroanisole (TeCA), 2,4,6-tribromoanisole (TBA) and pentachloroanisole (PCA), 2-methylisoborneol (2MIB) and geosmin (GSM) have been reported as being responsible for cork and wine taint. A sustainable method based on thermal desorption-gas chromatography–mass spectrometry (TD-GC/MS) has been developed and optimized, taking into account desorption parameters and chromatographic and mass spectrometric conditions. The combination of parameters that jointly maximized the compound detection was as follows: desorption temperature at 300 °C, desorption time at 30 min, cryo-temperature at 20 °C and trap high temperature at 305 °C. The proposed methodology showed a good linearity (R ≤ 0.994) within the tested range (from 0.1 to 2 ng) for all target compounds. The precision expressed as repeatability and reproducibility was RSD < 10% in both. The limits of quantification ranged from 0.05 to 0.1 ng. The developed methodology and the sampling rates (R-values) of all targeted compounds (from 0.013 to 0.071 m3 h−1) were applied to the air analysis of two wineries. The results showed that the developed methodology is a sustainable and useful tool for the determination of these compounds in air.
Climate Change, Addiction, and Spiritual Liberation
Authors: Bullitt-Jonas Margaret
Published: September 2021

Climate scientists have sounded the alarm: The only way to preserve a planet that is generally habitable for human beings is to carry out a transformation of society at a rate and scale that are historically unprecedented. Can we do this? Will we do this? Drawing on her long-term recovery from addiction and on her decades of ministry as a climate activist, the author reflects on how understanding the dynamics of addiction and recovery might inform our efforts to protect the web of life and to bear witness to the liberating God of love who makes all things new.
The “Closet” and “Out of the Closet” versus “Private Space” and “Public Space”: Indigenous Knowledge System as the Key to Understanding Same-Sex Sexualities in Rural Communities
Authors: Khuzwayo-Magwaza Lindiwe P
Published: September 2021

This paper is produced from the author’s Ph.D. project on indigenous same-sex sexualities. It interrogates the way same-sex sexualities or homosexuality is understood in the West and how the Western interpretations of sexualities and genders are imposed on African rural communities. The paper argues that such Western impositions impede our understanding of same-sex relationships, and it threatens any attempt made to bring sexual orientation awareness programmes to rural areas. The study is framed on African indigenous knowledge systems to accommodate African indigenous perspectives on same-sex sexualities. This approach introduces indigenised same-sex discourse into contemporary discourses. The study was conducted in rural communities of Kwa Zulu Natal (KZN), where families of same-sex individuals (SSI) reside. The research employed a qualitative methodology that involved SSI, families, traditional and Church leaders. Triangulation methods involve individual interviews, focus groups, and workshops, this method is meant to validate research findings. The results reveal that, before Western debates on African same-sex sexualities, the idea of the “closet or out of the closet” did not exist, instead people lived a private life. Meaning, approaching sexual discourse by respecting this choice gives people of different sexualities the privacy they want, and this is regarded as a “safe place” for them.