bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanistic Insight into the Enzymatic Inhibition of β-Amyrin against Mycobacterial Rv1636: In Silico and In Vitro Approaches
Authors: Beg Md Amjad , Sadaf  , Shamsi Anas , Sahoo Sibasis , Yousuf Mohd , Najm Mohammad Zeeshan , Almutawif Yahya Ahmad , Islam Asimul , Aloliqi Abdulaziz A. , Athar Fareeda
Published: August 2022

Mycobacterium tuberculosis has seen tremendous success as it has developed defenses to reside in host alveoli despite various host-related stress circumstances. Rv1636 is a universal stress protein contributing to mycobacterial survival in different host-derived stress conditions. Both ATP and cAMP can be bound with the Rv1636, and their binding actions are independent of one another. β-Amyrin, a triterpenoid compound, is abundant in medicinal plants and has many pharmacological properties and broad therapeutic potential. The current study uses biochemical, biophysical, and computational methods to define the binding of Rv1636 with β-Amyrin. A substantial interaction between β-Amyrin and Rv1636 was discovered by molecular docking studies, which helped decipher the critical residues involved in the binding process. VAL60 is a crucial residue found in the complexes of both Rv1636_β-Amyrin and Rv1636-ATP. Additionally, the Rv1636_β-Amyrin complex was shown to be stable by molecular dynamics simulation studies (MD), with minimal changes observed during the simulation. In silico observations were further complemented by in vitro assays. Successful cloning, expression, and purification of Rv1636 were accomplished using Ni-NTA affinity chromatography. The results of the ATPase activity assay indicated that Rv1636’s ATPase activity was inhibited in the presence of various β-Amyrin concentrations. Additionally, circular dichroism spectroscopy (CD) was used to examine modifications to Rv1636 secondary structure upon binding of β-Amyrin. Finally, isothermal titration calorimetry (ITC) advocated spontaneous binding of β-Amyrin with Rv1636 elucidating the thermodynamics of the Rv1636_β-Amyrin complex. Thus, the study establishes that β-Amyrin binds to Rv1636 with a significant affinity forming a stable complex and inhibiting its ATPase activity. The present study suggests that β-Amyrin might affect the functioning of Rv1636, which makes the bacterium vulnerable to different stress conditions.
Plasmodium falciparum Nicotinamidase as A Novel Antimalarial Target
Authors: Donu Dickson , Sharma Chiranjeev , Cen Yana
Published: August 2022

Inhibition of Plasmodium falciparum nicotinamidase could represent a potential antimalarial since parasites require nicotinic acid to successfully recycle nicotinamide to NAD+, and importantly, humans lack this biosynthetic enzyme. Recently, mechanism-based inhibitors of nicotinamidase have been discovered. The most potent compound inhibits both recombinant P. falciparum nicotinamidase and parasites replication in infected human red blood cells (RBCs). These studies provide evidence for the importance of nicotinamide salvage through nicotinamidase as a central master player of NAD+ homeostasis in P. falciparum.
Electrospun Zein/Polyoxyethylene Core-Sheath Ultrathin Fibers and Their Antibacterial Food Packaging Applications
Authors: Jiang Wenlai , Zhao Ping , Song Wenliang , Wang Menglong , Yu Deng-Guang
Published: August 2022

The purpose of this work is to develop a novel ultrathin fibrous membrane with a core-sheath structure as antibacterial food packaging film. Coaxial electrospinning was exploited to create the core-sheath structure, by which the delivery regulation of the active substance was achieved. Resveratrol (RE) and silver nanoparticles (AgNPs) were loaded into electrospun zein/polyethylene oxide ultrathin fibers to ensure a synergistic antibacterial performance. Under the assessments of a scanning electron microscope and transmission electron microscope, the ultrathin fiber was demonstrated to have a fine linear morphology, smooth surface and obvious core-sheath structure. X-ray diffraction and Fourier transform infrared analyses showed that RE and AgNPs coexisted in the ultrathin fibers and had good compatibility with the polymeric matrices. The water contact angle experiments were conducted to evaluate the hydrophilicity and hygroscopicity of the fibers. In vitro dissolution tests revealed that RE was released in a sustained manner. In the antibacterial experiments against Staphylococcus aureus and Escherichia coli, the diameters of the inhibition zone of the fiber were 8.89 ± 0.09 mm and 7.26 ± 0.10 mm, respectively. Finally, cherry tomatoes were selected as the packaging object and packed with fiber films. In a practical application, the fiber films effectively reduced the bacteria and decreased the quality loss of cherry tomatoes, thereby prolonging the fresh-keeping period of cherry tomatoes to 12 days. Following the protocols reported here, many new food packaging films can be similarly developed in the future.
Design, Synthesis and Activity of New N1-Alkyl Tryptophan Functionalized Dendrimeric Peptides against Glioblastoma
Authors: Sowińska Marta , Szeliga Monika , Morawiak Maja , Zabłocka Barbara , Urbanczyk-Lipkowska Zofia
Published: August 2022

Background: Due to resistance to conventional therapy, a blood–brain barrier that results in poor drug delivery, and a high potential for metastasis, glioblastoma (GBM) presents a great medical challenge. Since the repertoire of the possible therapies is very limited, novel therapeutic strategies require new drugs as well as new approaches. The multiple roles played by L-tryptophan (Trp) in tumorigenesis of GBM and the previously found antiproliferative properties of Trp-bearing dendrimers against this malignancy prompted us to design novel polyfunctional peptide-based dendrimers covalently attached to N1-alkyl tryptophan (Trp) residues. Their antiproliferative properties against GBM and normal human astrocytes (NHA) and their antioxidant potential were tested. Methods: Two groups of amphiphilic peptide dendrimers terminated with N1-butyl and N1-aminopentane tryptophan were designed. The influence of dendrimers on viability of NHA and human GBM cell lines, displaying different genetic backgrounds and tumorigenic potentials, was determined by the MTT test. The influence of compounds on the clonogenic potential of GBM cells was assessed by colony-formation assay. Dendrimers were tested for radical scavenging potency as well as redox capability (DPPH, ABTS, and FRAP models). Results: Several peptide dendrimers functionalized with N1-alkyl-tryptophan at 5 µM concentration exhibited high selectivity towards GBM cells retaining 85–95% viable NHA cells while killing cancer cells. In both the MTT and colony-formation assays, compounds 21 (functionalized with N1-butyl-Trp and (+)8 charged) and 25 (functionalized with N1-aminopentane-Trp and (+)12 charged) showed the most promise for their development into anticancer drugs. According to ABTS, DPPH, and FRAP antioxidant tests, dendrimers functionalized with N1-alkylated Trp expressed higher ROS-scavenging capacity (ABTS and DPPH) than those with unsubstituted Trp. Conclusions: Peptide dendrimers functionalized with N1-alkyl-tryptophan showed varying toxicity to NHA, while all were toxic to GBM cells. Based on their activity towards inhibition of GBM viability and relatively mild effect on NHA cells the most advantageous were derivatives 21 and 25 with the respective di-dodecyl and dodecyl residue located at the C-terminus. As expected, peptide dendrimers functionalized with N1-alkyl-tryptophan expressed higher scavenging potency against ROS than dendrimers with unsubstituted tryptophan.
Edaravone Attenuated Angiotensin II-Induced Atherosclerosis and Abdominal Aortic Aneurysms in Apolipoprotein E-Deficient Mice
Authors: Uchida Haruhito A. , Takatsuka Tetsuharu , Hada Yoshiko , Umebayashi Ryoko , Takeuchi Hidemi , Shikata Kenichi , Subramanian Venkateswaran , Daugherty Alan , Wada Jun
Published: August 2022

Background: The aim of the study was to define whether edaravone, a free-radical scavenger, influenced angiotensin II (AngII)-induced atherosclerosis and abdominal aortic aneurysms (AAAs) formation. Methods: Male apolipoprotein E-deficient mice (8–12 weeks old) were fed with a normal diet for 5 weeks. Either edaravone (10 mg/kg/day) or vehicle was injected intraperitoneally for 5 weeks. After 1 week of injections, mice were infused subcutaneously with either AngII (1000 ng/kg/min, n = 16–17 per group) or saline (n = 5 per group) by osmotic minipumps for 4 weeks. Results: AngII increased systolic blood pressure equivalently in mice administered with either edaravone or saline. Edaravone had no effect on plasma total cholesterol concentrations and body weights. AngII infusion significantly increased ex vivo maximal diameters of abdominal aortas and en face atherosclerosis but was significantly attenuated by edaravone administration. Edaravone also reduced the incidence of AngII-induced AAAs. In addition, edaravone diminished AngII-induced aortic MMP-2 activation. Quantitative RT-PCR revealed that edaravone ameliorated mRNA abundance of aortic MCP-1 and IL-1β. Immunostaining demonstrated that edaravone attenuated oxidative stress and macrophage accumulation in the aorta. Furthermore, edaravone administration suppressed thioglycolate-induced mice peritoneal macrophages (MPMs) accumulation and mRNA abundance of MCP-1 in MPMs in male apolipoprotein E-deficient mice. In vitro, edaravone reduced LPS-induced mRNA abundance of MCP-1 in MPMs. Conclusions: Edaravone attenuated AngII-induced AAAs and atherosclerosis in male apolipoprotein E-deficient mice via anti-oxidative action and anti-inflammatory effect.
A Setmelanotide-like Effect at MC4R Is Achieved by MC4R Dimer Separation
Authors: Reininghaus Nanina , Paisdzior Sarah , Höpfner Friederike , Jyrch Sabine , Cetindag Cigdem , Scheerer Patrick , Kühnen Peter , Biebermann Heike
Published: August 2022

Melanocortin 4 receptor (MC4R) is part of the leptin-melanocortin pathway and plays an essential role in mediating energy homeostasis. Mutations in the MC4R are the most frequent monogenic cause for obesity. Due to increasing numbers of people with excess body weight, the MC4R has become a target of interest in the search of treatment options. We have previously reported that the MC4R forms homodimers, affecting receptor Gs signaling properties. Recent studies introducing setmelanotide, a novel synthetic MC4R agonist, suggest a predominant role of the Gq/11 pathway regarding weight regulation. In this study, we analyzed effects of inhibiting homodimerization on Gq/11 signaling using previously reported MC4R/CB1R chimeras. NanoBRETTM studies to determine protein–protein interaction were conducted, confirming decreased homodimerization capacities of chimeric receptors in HEK293 cells. Gq/11 signaling of chimeric receptors was analyzed using luciferase-based reporter gene (NFAT) assays. Results demonstrate an improvement of alpha-MSH-induced NFAT signaling of chimeras, reaching the level of setmelanotide signaling at wild-type MC4R (MC4R-WT). In summary, our study shows that inhibiting homodimerization has a setmelanotide-like effect on Gq/11 signaling, with chimeric receptors presenting increased potency compared to MC4R-WT. These findings indicate the potential of inhibiting MC4R homodimerization as a therapeutic target to treat obesity.
Physicochemical, Morphological, and Functional Properties of Starches Isolated from Avocado Seeds, a Potential Source for Resistant Starch
Authors: Wang Jiashui , Li Yanxia , Jin Zhiqiang , Cheng Yunjiang
Published: August 2022

This study compared the physicochemical and functional properties of starches from eight cultivars of avocado seeds. Amylose content, morphology, crystalline structure, swelling power, solubility, thermal and pasting properties, and in vitro digestibility were investigated. The results revealed that the apparent amylose content of starches from avocado seeds varied with different varieties. Light microscopic and scanning electron microscopic examination demonstrated that the eight starches differed slightly in terms of morphology and granule size. The X-ray diffraction and Fourier transform infrared spectroscopy analyses showed that the crystal structure and chemical linkage of the avocado seed starches were similar. However, the pasting, water solubility, and thermal properties of the eight avocado seed starches differed. Importantly, all the starches had high resistant starch content (>60%), with the highest found in Hass seeds (77.83%). To conclude, starch from avocado seeds has a high potential for use in the production of resistant starch.
The Antimicrobial Properties of Cannabis and Cannabis-Derived Compounds and Relevance to CB2-Targeted Neurodegenerative Therapeutics
Authors: Hong HeeJue , Sloan Lucy , Saxena Deepak , Scott David A.
Published: August 2022

Cannabinoid receptor 2 (CB2) is of interest as a much-needed target for the treatment or prevention of several neurogenerative diseases. However, CB2 agonists, particularly phytocannabinoids, have been ascribed antimicrobial properties and are associated with the induction of microbiome compositional fluxes. When developing novel CB2 therapeutics, CB2 engagement and antimicrobial functions should both be considered. This review summarizes those cannabinoids and cannabis-informed molecules and preparations (CIMPs) that show promise as microbicidal agents, with a particular focus on the most recent developments. CIMP–microbe interactions and anti-microbial mechanisms are discussed, while the major knowledge gaps and barriers to translation are presented. Further research into CIMPs may proffer novel direct or adjunctive strategies to augment the currently available antimicrobial armory. The clinical promise of CIMPs as antimicrobials, however, remains unrealized. Nevertheless, the microbicidal effects ascribed to several CB2 receptor-agonists should be considered when designing therapeutic approaches for neurocognitive and other disorders, particularly in cases where such regimens are to be long-term. To this end, the potential development of CB2 agonists lacking antimicrobial properties is also discussed.
Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials
Authors: Capatina Denisa , Lupoi Teodora , Feier Bogdan , Olah Diana , Cristea Cecilia , Oprean Radu
Published: August 2022

The rapid diagnosis of Pseudomonas aeruginosa infection is very important because this bacterium is one of the main sources of healthcare-associated infections. Pseudomonas quinolone signal (PQS) is a specific molecule for quorum sensing (QS) in P. aeruginosa, a form of cell-to-cell bacterial communication and its levels can allow the determination of the bacterial population. In this study, the development of the first electrochemical detection of PQS using screen-printed electrodes modified with carbon nanotubes (CNT-SPE) is reported. The electrochemical fingerprint of PQS was determined using different electrode materials and screen-printed electrodes modified with different nanomaterials. The optimization of the method in terms of electrolyte, pH, and electrochemical technique was achieved. The quantification of PQS was performed using one of the anodic peaks in the electrochemical fingerprint of the PQS on the CNT-SPE. The sensor exhibited a linear range from 0.1 to 15 µM, with a limit of detection of 50 nM. The sensor allowed the selective detection of PQS, with low interference from other QS molecules. The sensor was successfully applied to analysis of real samples (spiked urine and human serum samples, spiked microbiological growth media, and microbiological cultures).
Computer-Based Assessment and Self-Report Measures of Executive Functions in High-Functioning Adults with Autism
Authors: Mihailescu Ilinca , Andrei Lucia Emanuela , Frunza Alina Alexandra , Manea Mirela , Rad Florina
Published: August 2022

This study analyzes the profile of executive functions (EF) in high-functioning adults with autism (HFA), both in terms of performance on four computer-based tasks, as well as how these functions are perceived by the individuals through self-reporting measures. The study included 64 participants: 32 individuals with HFA, and 32 typically developing controls. Four CANTAB tasks were used (assessing spatial working memory, planning, visual memory, and inhibition), as well as a self-reported measure of executive functions (BDEFS) and a scale for the severity of autism symptoms (RAADS-R). The participants in the ASD group performed significantly lower than the control group on all four computer-based tasks, as measured by the total number of errors made (for the spatial working memory, visual memory, and inhibition tasks) and the number of problems solved at the first choice (for the planning task). No correlation was found in the ASD group between the severity of autism symptoms and the computer-based measures. These findings provide evidence that HFA adults may have various executive functioning impairments, and subsequent daily life problems, but these deficits do not necessarily correlate with the severity of core ASD symptoms.
Talking about Health: A Topic Analysis of Narratives from Individuals with Schizophrenia and Other Serious Mental Illnesses
Authors: Cowan Tovah , Rodriguez Zachary B. , Granrud Ole Edvard , Masucci Michael D. , Docherty Nancy M. , Cohen Alex S.
Published: August 2022

Individuals with schizophrenia have higher mortality and shorter lifespans. There are a multitude of factors which create these conditions, but one aspect is worse physical health, particularly cardiovascular and metabolic health. Many interventions to improve the health of individuals with schizophrenia have been created, but on the whole, there has been limited effectiveness in improving quality of life or lifespan. One potential new avenue for inquiry involves a more patient-centric perspective; understanding aspects of physical health most important, and potentially most amenable to change, for individuals based on their life narratives. This study used topic modeling, a type of Natural Language Processing (NLP) on unstructured speech samples from individuals (n = 366) with serious mental illness, primarily schizophrenia, in order to extract topics. Speech samples were drawn from three studies collected over a decade in two geographically distinct regions of the United States. Several health-related topics emerged, primarily centered around food, living situation, and lifestyle (e.g., routine, hobbies). The implications of these findings for how individuals with serious mental illness and schizophrenia think about their health, and what may be most effective for future health promotion policies and interventions, are discussed.
Improved YOLOv3-Based Bridge Surface Defect Detection by Combining High- and Low-Resolution Feature Images
Authors: Teng Shuai , Liu Zongchao , Li Xiaoda
Published: August 2022

Automatic bridge surface defect detection is of wide concern; it can save human resources and improve work efficiency. The object detection algorithm, especially the You Only Look Once (YOLO) series of networks, has important potential in real-time object detection because of its fast detection speed, and it provides an efficient and automatic detection method for bridge surface defect detection. Hence, this paper employs an improved YOLOv3 network for detecting bridge surface defects (cracks and exposed rebar) and compares the effects of the advanced YOLOv2, YOLOv3 and faster region-based convolutional neural network (Faster RCNN) in bridge surface defect detection, and then two approaches (transfer learning and data augmentation) are used to improve the YOLOv3. The results confirm that, by combining high- and low-resolution feature images, the YOLOv3 improves the detection effect of the YOLOv2 (using single-resolution feature images); the average precision (AP) value of the improved YOLOv3 (0.9–0.91) is 6–10% higher than that of the YOLOv2 (0.83–0.86). Then, the anti-noise abilities of the YOLOv2 and YOLOv3 are studied by introducing white Gaussian noise, and the YOLOv3 is better than the YOLOv2. Simultaneously, the YOLO series of detectors perform better in detection speed; the detection speed of the improved YOLOv3 (FPS (frames per second) = 23.8) is 103 times that of the Faster RCNN (FPS = 0.23) with comparable mAP values (improved YOLOv3 = 0.91; Faster RCNN = 0.9). It is demonstrated that, in consideration of detection precision and speed, the proposed improved YOLOv3 is a decent detector for fast and real-time bridge defect detection.
Human-Induced-Vibration Response Analysis and Comfort Evaluation Method of Large-Span Steel Vierendeel Sandwich Plate
Authors: Jiang Lan , Cheng Ruoheng , Zhang Huagang , Ma Kejian
Published: August 2022

A steel Vierendeel sandwich plate used as a large-span lightweight floor structure for vibration comfort during crowd gatherings was considered. Taking the steel Vierendeel sandwich plate in Guizhou Museum as an example, through finite element transient analysis, the effects of the structural damping, pedestrian self-weight, floor span, surface concrete slab thickness, and structural parameters on the floor’s acceleration response distribution were deeply studied. According to the distribution characteristics of the acceleration response, a distribution model function was constructed, and a distribution Gauss model of the relationship between the peak acceleration response and the position of the steel Vierendeel sandwich plate was established. A field test of the sandwich plate under human-induced fixed-point excitation was carried out, and the model fitting results were compared with the actual test results. The results showed that the Gaussian model could effectively estimate the peak acceleration response at different positions on the floor. In addition, according to the distribution model, a comfort evaluation method based on the comfort assurance rate was proposed that could greatly reduce the representative value of the acceleration evaluation. The research results provide a reference for the comfort evaluation and corresponding vibration-reduction design of long-span steel Vierendeel sandwich plates.
Efficient Removal of Organic Dye from Aqueous Solution Using Hierarchical Zeolite-Based Biomembrane: Isotherm, Kinetics, Thermodynamics and Recycling Studies
Authors: Radoor Sabarish , Karayil Jasila , Jayakumar Aswathy , Lee Jaewoo , Nandi Debabrata , Parameswaranpillai Jyotishkumar , Pant Bishweshwar , Siengchin Suchart
Published: August 2022

Bio adsorbents have received tremendous attention due to their eco-friendly, cheap and non-toxic nature. Recently, bio-adsorbent-based membranes have been frequently employed for water treatment. The work reports the preparation of a novel adsorbent membrane from hierarchical zeolite, polyvinyl alcohol, carboxymethyl cellulose and agar. The fabricated membrane was characterized spectroscopically and microscopically with several techniques such as XRD, UTM, TGA, optical microscopy and FT-IR, as well as contact-angle studies. The result showed that the hierarchical-zeolite-loaded membrane is superior in terms of thermal stability, mechanical properties and surface roughness. The fabricated membrane was investigated for its efficiency in the removal of Congo red dye in aqueous conditions. The influence of pH, temperature, contact period and the initial concentration of dye and zeolite loading on the adsorption process are also explored. The adsorption results highlighted the maximum sorption property of Congo red on agar/zeolite/carboxymethyl cellulose/polymer biomembrane was found to be higher (15.30 mg/g) than that of zeolite powder (6.4 mg/g). The adsorption isotherms and kinetic parameters were investigated via Langmuir, Freundlich and pseudo-first order, pseudo-second order and the intraparticle diffusion model, respectively. The adsorption isotherms fitted well for both considered isotherms, whereas pseudo-second order fitted well for kinetics. The thermodynamic parameter, ΔG at 303 K, 313 K and 323 K was −9.12, −3.16 and −0.49 KJ/mol, respectively. The work further explores the antibacterial efficacy of the prepared membrane and its reusability.
Biomass-Derived Carbon Anode for High-Performance Microbial Fuel Cells
Authors: Moradian Jamile Mohammadi , Wang Songmei , Ali Amjad , Liu Junying , Mi Jianli , Wang Hongcheng
Published: August 2022

Although microbial fuel cells (MFCs) have been developed over the past decade, they still have a low power production bottleneck for practical engineering due to the ineffective interfacial bioelectrochemical reaction between exoelectrogens and anode surfaces using traditional carbonaceous materials. Constructing anodes from biomass is an effective strategy to tackle the current challenges and improve the efficiency of MFCs. The advantage features of these materials come from the well-decorated aspect with an enriched functional group, the turbostratic nature, and porous structure, which is important to promote the electrocatalytic behavior of anodes in MFCs. In this review article, the three designs of biomass-derived carbon anodes based on their final products (i.e., biomass-derived nanocomposite carbons for anode surface modification, biomass-derived free-standing three-dimensional carbon anodes, and biomass-derived carbons for hybrid structured anodes) are highlighted. Next, the most frequently obtained carbon anode morphologies, characterizations, and the carbonization processes of biomass-derived MFC anodes were systematically reviewed. To conclude, the drawbacks and prospects for biomass-derived carbon anodes are suggested.
Endothelial Cell Dysfunction and Nonalcoholic Fatty Liver Disease (NAFLD): A Concise Review
Authors: Nasiri-Ansari Narjes , Androutsakos Theodoros , Flessa Christina-Maria , Kyrou Ioannis , Siasos Gerasimos , Randeva Harpal S. , Kassi Eva , Papavassiliou Athanasios G.
Published: August 2022

Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases worldwide. It is strongly associated with obesity, type 2 diabetes (T2DM), and other metabolic syndrome features. Reflecting the underlying pathogenesis and the cardiometabolic disorders associated with NAFLD, the term metabolic (dysfunction)-associated fatty liver disease (MAFLD) has recently been proposed. Indeed, over the past few years, growing evidence supports a strong correlation between NAFLD and increased cardiovascular disease (CVD) risk, independent of the presence of diabetes, hypertension, and obesity. This implies that NAFLD may also be directly involved in the pathogenesis of CVD. Notably, liver sinusoidal endothelial cell (LSEC) dysfunction appears to be implicated in the progression of NAFLD via numerous mechanisms, including the regulation of the inflammatory process, hepatic stellate activation, augmented vascular resistance, and the distortion of microcirculation, resulting in the progression of NAFLD. Vice versa, the liver secretes inflammatory molecules that are considered pro-atherogenic and may contribute to vascular endothelial dysfunction, resulting in atherosclerosis and CVD. In this review, we provide current evidence supporting the role of endothelial cell dysfunction in the pathogenesis of NAFLD and NAFLD-associated atherosclerosis. Endothelial cells could thus represent a “golden target” for the development of new treatment strategies for NAFLD and its comorbid CVD.
Nano Modification of Antrodia Cinnamomea Exhibits Anti-Inflammatory Action and Improves the Migratory Potential of Myogenic Progenitors
Authors: Menon Mridula P. , Chien Yi-Hsuan , Thomas Joy , Yu Yu-Hsiang , Chang Chang-Tang , Hua Kuo-Feng
Published: August 2022

The skeletal muscle progenitors’ proliferation and migration are crucial stages of myogenesis. Identifying drug candidates that contribute to myogenesis can have a positive impact on atrophying muscle. The purpose of the study is to synthesize the Antrodia cinnamomea (AC)-β-cyclodextrin (BCD) inclusion complex (IC) and understand its in vitro pro-regenerative influence in murine skeletal C2C12 myoblasts. The IC was subjected to various nano-characterization studies. Fluorescent IC was synthesized to understand the cellular uptake of IC. Furthermore, 25 µg/mL, 12.5 µg/mL, and 6.25 µg/mL of IC were tested on murine C2C12 skeletal muscle cells for their anti-inflammatory, pro-migratory, and pro-proliferative action. The cellular internalization of IC occurred rapidly via pinocytosis. IC (252.6 ± 3.2 nm size and −37.24 ± 1.55 surface charge) exhibited anti-inflammatory action by suppressing the secretion of interleukin-6 and enhanced cell proliferation with promising cytocompatibility. A 12.5 μg/mL dose of IC promoted cell migration in 24 h, but the same dose of AC significantly reduced cell migration, suggesting modification by BCD. Molecular studies revealed that IC promoted C2C12 myoblasts migration by upregulating long non-coding RNA (lncRNA) NEAT-1, SYISL, and activating the pPKC/β-catenin pathway. Our study is the first report on the pro-proliferative and pro-migratory effects of BCD-modified extracts of AC.
Autophagy, Acute Pancreatitis and the Metamorphoses of a Trypsinogen-Activating Organelle
Authors: Voronina Svetlana , Chvanov Michael , De Faveri Francesca , Mayer Ulrike , Wileman Tom , Criddle David , Tepikin Alexei
Published: August 2022

Recent studies have highlighted the importance of autophagy and particularly non-canonical autophagy in the development and progression of acute pancreatitis (a frequent disease with considerable morbidity and significant mortality). An important early event in the development of acute pancreatitis is the intrapancreatic activation of trypsinogen, (i.e., formation of trypsin) leading to the autodigestion of the organ. Another prominent phenomenon associated with the initiation of this disease is vacuolisation and specifically the formation of giant endocytic vacuoles in pancreatic acinar cells. These organelles develop in acinar cells exposed to several inducers of acute pancreatitis (including taurolithocholic acid and high concentrations of secretagogues cholecystokinin and acetylcholine). Notably, early trypsinogen activation occurs in the endocytic vacuoles. These trypsinogen-activating organelles undergo activation, long-distance trafficking, and non-canonical autophagy. In this review, we will discuss the role of autophagy in acute pancreatitis and particularly focus on the recently discovered LAP-like non-canonical autophagy (LNCA) of endocytic vacuoles.
p73α1, an Isoform of the p73 Tumor Suppressor, Modulates Lipid Metabolism and Cancer Cell Growth via Stearoyl-CoA Desaturase-1
Authors: Rabow Zachary , Laubach Kyra , Kong Xiangmudong , Shen Tong , Mohibi Shakur , Zhang Jin , Fiehn Oliver , Chen Xinbin
Published: August 2022

Altered lipid metabolism is a hallmark of cancer. p73, a p53 family member, regulates cellular processes and is expressed as multiple isoforms. However, the role of p73 in regulating lipid metabolism is not well-characterized. Previously, we found that loss of p73 exon 12 (E12) leads to an isoform switch from p73α to p73α1, the latter of which has strong tumor suppressive activity. In this study, comprehensive untargeted metabolomics was performed to determine whether p73α1 alters lipid metabolism in non-small cell lung carcinoma cells. RNA-seq and molecular biology approaches were combined to identify lipid metabolism genes altered upon loss of E12 and identify a direct target of p73α1. We found that loss of E12 leads to decreased levels of phosphatidylcholines, and this was due to decreased expression of genes involved in phosphatidylcholine synthesis. Additionally, we found that E12-knockout cells had increased levels of phosphatidylcholines containing saturated fatty acids (FAs) and decreased levels of phosphatidylcholines containing monounsaturated fatty acids (MUFAs). We then found that p73α1 inhibits cancer cell viability through direct transcriptional suppression of Stearoyl-CoA Desaturase-1 (SCD1), which converts saturated FAs to MUFAs. Finally, we showed that p73α1-mediated suppression of SCD1 leads to increased ratios of saturated FAs to MUFAs.
Transcriptional Factors and Epigenetic Mechanisms in Obesity and Related Metabolic Comorbidities
Authors: Zaiou Mohamed
Published: August 2022

Recent advances in the study of chromatin remodeling and transcriptional machinery complex dysfunction, and how they drive metabolic-related gene expression have considerably increased our understanding of several molecular processes underlaying obesity and its complications [...]
Proline Enantiomers Discrimination by (L)-Prolinated Porphyrin Derivative Langmuir–Schaefer Films: Proof of Concept for Chiral Sensing Applications
Authors: Giancane Gabriele , Pagano Rosanna , Naitana Mario Luigi , Magna Gabriele , Stefanelli Manuela , Monti Donato , Paolesse Roberto , Bettini Simona , Valli Ludovico
Published: August 2022

A porphyrin derivative functionalized with the L-enantiomer of proline amino acid was characterized at the air–pure water interface of the Langmuir trough. The porphyrin derivative was dissolved in dichloromethane solution, spread at the air–subphase interface and investigated by acquiring the surface pressure vs. area per molecule Langmuir curves. It is worth observing that the behavior of the molecules of the porphyrin derivative floating film was substantially influenced by the presence of L-proline amino acid dissolved in the subphase (10−5 M); on the contrary, the physical chemical features of the floating molecules were only slightly influenced by the D-proline dissolved in the subphase. Such an interesting chirality-driven selection was preserved when the floating film was transferred onto solid supports by means of the Langmuir–Schaefer method, but it did not emerge when a spin-coating technique was used for the layering of the tetrapyrrolic derivatives. The obtained results represent proof of concept for the realization of active molecular layers for chiral discrimination: porphyrin derivatives, due to their intriguing spectroscopic and supramolecular properties, can be functionalized with the chiral molecule that should be detected. Moreover, the results emphasize the crucial role of the deposition technique on the features of the sensing layers.
PTSD Risk Factors and Acute Pain Intensity Predict Length of Hospital Stay in Youth after Unintentional Injury
Authors: Agoston Anna , Bhatia Amina , Bleacher John , Smith Alexis , Hill Karen , Edwards Susanne , Cochran Alicia , Routly Maia
Published: August 2022

Background: Many hospitals have adopted screening tools to assess risk for posttraumatic stress disorder (PTSD) after pediatric unintentional injury in accordance with American College of Surgeons recommendations. The Screening Tool for Early Predictors of PTSD (STEPP) is a measure initially developed to identify youth and parents at high risk for meeting diagnostic criteria for PTSD after injury. Acute pain during hospitalization has also been examined as a potential predictor of maladaptive outcomes after injury, including PTSD. We investigated in a retrospective cohort study whether the STEPP, as well as acute pain intensity during hospitalization, would predict maladaptive outcomes during the peri-trauma in addition to the post-trauma period, specifically length of hospitalization. Methods: A total of 1123 youths aged 8–17 (61% male) and their parents were included. Patients and parents were administered the STEPP for clinical reasons while hospitalized. Acute pain intensity and length of stay were collected through retrospective chart review. Results: Adjusting for demographics and injury severity, child but not parent STEPP total predicted length of stay. Acute pain intensity, child threat to life appraisal, and child pulse rate predicted length of stay. Conclusions: Acute pain intensity and child PTSD risk factors, most notably child threat to life appraisal, predicted hospitalization length above and beyond multiple factors, including injury severity. Pain intensity and child appraisals may not only serve as early warning signs for maladaptive outcomes after injury but also indicate a more difficult trajectory during hospitalization. Additional assessment and treatment of these factors may be critical while youth are hospitalized. Utilizing psychology services to support youth and integrating trauma-informed care practices during hospitalization may support improved outcomes for youth experiencing unintentional injury.
LifeLab: Co-Design of an Interactive Health Literacy Intervention for Socioeconomically Disadvantaged Adolescents’
Authors: Smith Craig , Goss Hannah R. , Issartel Johann , Meegan Sarah , Belton Sarahjane
Published: August 2022

Low socioeconomic populations, when compared to more affluent groups, are at greater risk of initiating risky behaviours and consequently developing health complications. Health literacy has been identified as a possible means to improve and sustain positive health behaviours, with adolescence being a time point when such behaviours can be embedded. To develop a meaningful health intervention, it has been recommended that relevant stakeholders be included in the design phase. This formative evaluation study was the second phase of co-design of an engaging health literacy intervention ‘LifeLab’ with, and for, socioeconomically disadvantaged adolescents in Ireland. In Spring 2021, a series of co-design workshops (n = 17) were facilitated with a convenience sample of adolescents from socially disadvantaged areas (n = 22) to gather their perceptions, feedback, and suggested changes on the LifeLab learning activities that had emerged from Phase 1 of the work. The data was analysed using reflexive thematic analysis, resulting in the development of three themes: (i) preferred learning engagement strategies, (ii) practical and logistical considerations and (iii) ideas for LifeLab content. The results highlight the value in adopting a participatory approach, as participants offered an array of suggestions and details to maximise the potential for LifeLab to be contextually relevant and engaging; suggestions which will directly inform the development and implementation of the intervention.
Basic Analysis of the Cerebrospinal Fluid: An Important Framework for Laboratory Diagnostics of the Impairment of the Central Nervous System
Authors: Kelbich Petr , Hrach Karel , Spicka Jan , Vachata Petr , Radovnicky Tomas , Hanuljakova Eva , Krejsek Jan
Published: August 2022

Laboratory analysis of basic cerebrospinal fluid (CSF) parameters is considered as essential for any CSF evaluation. It can provide rapidly very valuable information about the status of the central nervous system (CNS). Our retrospective study evaluated parameters of basic CSF analysis in cases of either infectious or non-infectious CNS involvement. Neutrophils are effector cells of innate immunity. Predominance of neutrophils was found in 98.2% of patients with purulent inflammation in CNS. Lymphocytes are cellular substrate of adaptive immunity. We found their predominance in 94.8% of patients with multiple sclerosis (MS), 66.7% of patients with tick-borne encephalitis (TBE), 92.2% of patients with neuroborreliosis, 83.3% of patients with inflammatory response with oxidative burst of macrophages in CNS and 75.0% of patients with malignant infiltration of meninges (MIM). The simultaneous assessment of aerobic and anaerobic metabolism in CSF using the coefficient of energy balance (KEB) allows us to specify the type of inflammation in CNS. We found predominantly aerobic metabolism (KEB > 28.0) in 100.0% CSF of patients with normal CSF findings and in 92.8% CSF of patients with MS. Predominant faintly anaerobic metabolism (28.0 > KEB > 20.0) in CSF was found in 71.8% patients with TBE and in 64.7% patients with neuroborreliosis. Strong anaerobic metabolism (KEB < 10.0) was found in the CSF of 99.1% patients with purulent inflammation, 100.0% patients with inflammatory response with oxidative burst of macrophages and in 80.6% patients with MIM. Joint evaluation of basic CSF parameters provides sufficient information about the immune response in the CSF compartment for rapid and reliable diagnosis of CNS involvement.
Special Issue: New Challenges in Thin-Film Nanocomposite Membranes
Authors: Meier-Haack Jochen
Published: August 2022

Rapid population growth and the associated rise in industrialization and food production have resulted in a tremendously increased demand for clean water.
Special Issue: Coatings and Thin Films as Functional and Protective Structures in Food Technology and Their Potential in Packaging Applications
Authors: Kocot Aleksandra Maria
Published: August 2022

One of the main challenges of the modern world is coping with the problem of antibiotic resistance [...]
Strength of Fiber Posts with Experimental TiO2 and ZrO2 Particle Bonding—An SEM, EDX, Rheometric and Push-Out Strength Study
Authors: Al-Saleh Samar , Vohra Fahim , Alateeq Abdullah , Alshaya Abdulaziz H. , Alotaibi Mohammed S. , Alhamdan Mai M. , Alrabiah Mohammed , Alsamrani Abdul , Abduljabbar Tariq
Published: August 2022

The present study aimed to prepare experimental adhesives (EAs): 5 wt.% titanium dioxide (TiO2) adhesive; and 5 wt.% zirconium oxide (ZrO2) adhesive; and analyze their impact on bond integrity of fiber posts to root dentin, and viscosity. The EA was composed of: bisphenol A glycol dimethacrylate (BisGMA); triethylene glycol dimethacrylate (TEGDMA); 2-hydroxyethyl methacrylate (HEMA); and ethyl 4-dimethylamino benzoate and camphorquinone. TiO2 and ZrO2 particles were individually incorporated into the EA at 5 wt.%, to form two groups (5% TiO2 and 5% ZrO2). The adhesives, with particles, were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy. The bonded interface was evaluated for adhesive–dentin penetration at the interface, using SEM. The study adhesive groups (EA, 5% TiO2 and 5% ZrO2) were analyzed for rheology and push-out bond strength of the fiber post to root dentin. Data were analyzed using analysis of variance and post hoc comparison. Both TiO2 and ZrO2 particles had irregular, non-uniform shapes. The EDX mapping showed the elemental presence of Ti (TiO2), Zr (ZrO2) and oxygen in formulated adhesives. The 5% TiO2 and 5% ZrO2 adhesives showed a decrease in viscosity, compared with the EA. Bond strength among the 5% TiO2 and 5% ZrO2 adhesives was statistically comparable (p > 0.05), but higher than the control group (10.57 ± 1.45 MPa) (p < 0.05). Reinforcement of the experimental dentin adhesive with 5% TiO2 or 5% ZrO2 increased the push-out bond strength of the fiber post to root dentin, in comparison with the EA. Particle-incorporated adhesives (5% TiO2 and 5% ZrO2) displayed decreased viscosity, compared with the EA (without particles).
Room-Temperature Synthesis of Titanium Nitride Using Metastable Nitrogen
Authors: Anderson Mark
Published: August 2022

The room-temperature synthesis of titanium nitride (TiN) is presented using the reaction of metastable nitrogen (MSN) with a titanium metal surface. The MSN is generated in a nitrogen glow discharge with plasma-ion filtering using a commercial direct analysis in real time (DART) source. The MSN is flowed over a titanium substrate at ambient pressure producing TiN surfaces that are ultra-clean and suitable for plasmonic applications. This is demonstrated using surface-enhanced infrared absorption spectroscopy (SEIRA), producing a 100-fold signal enhancement. Nitriding using MSN could find general applications in producing nitrided surfaces with small-scale structures.
Demonstration of III-Nitride Red LEDs on Si Substrates via Strain-Relaxed Template by InGaN Decomposition Layer
Authors: Rienzi Vincent , Smith Jordan , Lim Norleakvisoth , Chang Hsun-Ming , Chan Philip , Wong Matthew S. , Gordon Michael J. , DenBaars Steven P. , Nakamura Shuji
Published: August 2022

A III-nitride red LED with an active region temperature of 835 °C on a Si substrate utilizing a strain-relaxed template (SRT) is demonstrated. The peak wavelength blueshifts from 670 nm at 1 A/cm2 to 636 nm at 150 A/cm2. The on-wafer external quantum efficiency was 0.021% at 7 A/cm2 with an emission wavelength of 655 nm. The LED grown on a Si substrate exhibited a 116 nm redshift when compared to a co-loaded LED grown on sapphire. This is attributed to the difference in strain state for the III-nitride layers grown on Si compared to sapphire, allowing for more indium to be incorporated in the LED grown on Si. This suggests efficient III-nitride red LEDs and µLEDs on Si with a SRT can be realized with further material, device structure, and processing optimizations.
The Potential Effect of Climate Change on the Distribution of Endemic Anurans from Mexico’s Tropical Dry Forest
Authors: Ballesteros-Barrera Claudia , Tapia-Pérez Oscar , Zárate-Hernández Rocío , Leyte-Manrique Adrián , Martínez-Bernal Angélica , Vargas-Miranda Bárbara , Martínez-Coronel Matías , Ortiz-Burgos Selene
Published: August 2022

Mexico is one of the richest countries in amphibian species (420 spp.), with a high level of endemism (69%). The order Anura represents the most diverse and widespread of the three extant amphibian orders (257 spp.). The anurofauna of Mexico’s tropical dry forest ecosystem host a high proportion of the species and endemism registered in the country. In terms of conservation, both dry forests and amphibians are at risk due to climate change because it is expected that as the temperature becomes higher and precipitation decreases, this vegetation type may experience water stress. We applied the MaxEnt algorithm to estimate the potential current and future (year 2070) geographic distribution patterns of 95 endemic Mexican anuran species inhabiting the country’s tropical dry forests by considering two representative concentration pathway scenarios (RCP4.5/RCP8.5) and analyzed the potential distributional pattern changes. The results indicated that overall, species would experience enough of a significant warming effect to cause a reduction in the original distribution area, with 44% of species losing an average of 50% of their original range (9 spp. in threatened category); additionally, 22% of the species in the dry forest ecosystem will experience an average increase of almost 50% in their original area, two species will lose more than 80% of their range, and one will disappear.
Double Gain: The Radio Frequency Catheter Ablation of Ventricular Aneurysm Related Recurrent Ventricular Tachycardia on a Tremendous Cardiac Outpouching
Authors: Li Kexin , Jiang Yufeng , Huang Ziyin , Zhou Yafeng
Published: August 2022

Dilated cardiomyopathy (DCM) is a classic type of non-ischemic cardiomyopathy. Of these, idiopathic cardiomyopathy (IDCM) is a rare type of non-genetic dilated cardiomyopathy. More specifically, the patient had suspected IDCM combined with sustained polymorphic ventricular tachycardia (PMVT) of left ventricular basal segmental origin, cardiac systolic dysfunction and an ejection fraction (EF) of 29%. He had an abnormally large ventricular aneurysm (VA) in the posterior wall of the left ventricle with left ventricular end diastolic dimension (LVDd) of 90 mm. We performed an endocardial radiofrequency catheter ablation (RFCA) of the patient’s recurrent ventricular tachycardia (VT) on the basis of an implantable cardioverter (ICD). Although minimally invasive RFCA also carries a high risk, it is currently a two-pronged option to improve the patient’s quality of life and to prevent the recurrence of VT. Postoperatively, the patient was routinely given optimal anti-arrhythmic and heart failure (HF) treatments to improve cardiac function as well as being followed up for 9 months. The patient’s EF ascended to 36% without any recurrence of VT. In summary, RFCA of suspected IDCM combined with VA and VT of basal area origin would be an effective treatment.