bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Transmembrane Protein Shape on Surrounding Lipid Domain Formation by Wetting
Authors: Molotkovsky Rodion J. , Galimzyanov Timur R. , Batishchev Oleg V. , Akimov Sergey A.
Published: November 2019

Signal transduction through cellular membranes requires the highly specific and coordinated work of specialized proteins. Proper functioning of these proteins is provided by an interplay between them and the lipid environment. Liquid-ordered lipid domains are believed to be important players here, however, it is still unclear whether conditions for a phase separation required for lipid domain formation exist in cellular membranes. Moreover, membrane leaflets are compositionally asymmetric, that could be an obstacle for the formation of symmetric domains spanning the lipid bilayer. We theoretically show that the presence of protein in the membrane leads to the formation of a stable liquid-ordered lipid phase around it by the mechanism of protein wetting by lipids, even in the absence of conditions necessary for the global phase separation in the membrane. Moreover, we show that protein shape plays a crucial role in this process, and protein conformational rearrangement can lead to changes in the size and characteristics of surrounding lipid domains.
Total Phenolic and Flavonoid Content and Biological Activities of Extracts and Isolated Compounds of Cytisus villosus Pourr.
Authors: Larit Farida , León Francisco , Benyahia Samira , Cutler Stephen
Published: November 2019

The aim of this study was to evaluate the total phenolic and flavonoid content, and the in vitro antioxidant, anti-inflammatory, antibacterial, antifungal, antimalarial, cytotoxicity, and antiprotozoal activities of the Algerian plant Cytisus villosus Pourr. (Syn. Cytisus triflorus L’Hérit.). Additionally, the radioligand displacement affinity on opioid and cannabinoid receptors was assessed for the extracts and isolated pure compounds. The hydro alcoholic extract of the aerial part of C. villosus was partitioned with chloroform (CHCl3), ethyl acetate (EtOAc), and n-butanol (n-BuOH). The phenolic content of the C. villosus extracts was evaluated using a modified Folin–Ciocalteau method. The total flavonoid content was measured spectrometrically using the aluminum chloride colorimetric assay. The known flavonoids genistein (1), chrysin (2), chrysin-7-O-β-d-glucopyranoside (3), and 2″-O-α-l-rhamnosylorientin (4) were isolated. The antioxidant activities of the extracts and isolated compounds were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DDPH) and cellular antioxidant activity (CAA) assays. The plant extracts showed moderate antioxidant activity. EtOAc and n-BuOH extracts showed moderate anti-inflammatory activity through the inhibition of induced nitric oxide synthase (iNOS) with IC50 values of 48 and 90 µg/mL, respectively. The isolated pure compounds 1 and 3 showed good inhibition of Inducible nitric oxide synthase (iNOS) with IC50 values of 9 and 20 µg/mL, respectively. Compounds 1 and 2 exhibited lower inhibition of Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) with IC50 values of 28 and 38 µg/mL, respectively. Furthermore, the extracts and isolated pure compounds have been shown to exhibit low affinity for cannabinoid and opioid receptors. Finally, n-BuOH extract was a potent inhibitor of Trypanosoma brucei with IC50 value of 7.99 µg/mL and IC90 value of 12.61 µg/mL. The extracts and isolated compounds showed no antimicrobial, antimalarial nor antileishmanial activities. No cytotoxic effect was observed on cancer cell lines. The results highlight this species as a promising source of anti-inflammatory and antitrypanosomal agents.
Strategy for the Biosynthesis of Short Oligopeptides: Green and Sustainable Chemistry
Authors: Wang Tao , Zhang Yu-Ran , Liu Xiao-Huan , Ge Shun , Zhu You-Shuang
Published: November 2019

Short oligopeptides are some of the most promising and functionally important amide bond-containing components, with widespread applications. Biosynthesis of these oligopeptides may potentially become the ultimate strategy because it has better cost efficiency and environmental-friendliness than conventional solid phase peptide synthesis and chemo-enzymatic synthesis. To successfully apply this strategy for the biosynthesis of structurally diverse amide bond-containing components, the identification and selection of specific biocatalysts is extremely important. Given that perspective, this review focuses on the current knowledge about the typical enzymes that might be potentially used for the synthesis of short oligopeptides. Moreover, novel enzymatic methods of producing desired peptides via metabolic engineering are highlighted. It is believed that this review will be helpful for technological innovation in the production of desired peptides.
Development of Microalgae Biosensor Chip by Incorporating Microarray Oxygen Sensor for Pesticides Sensing
Authors: Kashem Md. Abul , Kimoto Kazuki , Iribe Yasunori , Suzuki Masayasu
Published: November 2019

A microalgae (Pseudokirchneriella subcapitata) biosensor chip for pesticide sensing has been developed by attaching the immobilized microalgae biofilm pon the microarray dye spots (size 100 μm and pitch 200 μm). The dye spots (ruthenium complex) were printed upon SO3-modified glass slides using a polydimethylsiloxane (PDMS) stamp and a microcontact printer (μCP). Emitted fluorescence intensity (FI) variance due to photosynthetic activity (O2 production) of microalgae was monitored by an inverted fluorescent microscope and inhibition of the oxygen generation rate was calculated based on the FI responses both before and after injection of pesticide sample. The calibration curves, as the inhibition of oxygen generation rate (%) due to photosynthetic activity inhibition by the pesticides, depicted that among the 6 tested pesticides, the biosensor showed good sensitivity for 4 pesticides (diuron, simetryn, simazine, and atrazine) but was insensitive for mefenacet and pendimethalin. The detection limits were 1 ppb for diuron and 10 ppb for simetryn, simazine, and atrazine. The simple and low-cost nature of sensing of the developed biosensor sensor chip has apparently created opportunities for regular water quality monitoring, where pesticides are an important concern.
Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay
Authors: Prante Marc , Schüling Torsten , Roth Bernhard , Bremer Kort , Walter Johanna
Published: November 2019

Detection of the small molecule 25-hydroxyvitamin D (25(OH)D) as the most relevant marker for vitamin D supply suffers from a high variability of results using the current detection methods, such as high-performance liquid chromatography (HPLC) and immunoassays. A new detection approach using a highly specific aptamer directed against 25(OH)D was established in this study based on the target-induced dissociation (TID) sensing approach. In this work, the aptamer was investigated regarding its structural properties as well as its binding affinity by using microscale thermophoresis (MST). Moreover, complementary oligonucleotides were designed based on the aptamer structure and were evaluated in MST experiments. Binding experiments of immobilized aptamers were conducted in microarray experiments. It could be shown that the aptamer exhibited the usual B-DNA structure and did not form any G-quadruplexes. The design of complementary oligonucleotides for the TID assay identified a putative 25(OH)D binding site within the aptamer. The limit of detection of the established competitive assay was determined to be 5.4 nM, which sets the stage for the development of a biosensor system.
Construction of a Hydrogel Pectin-Based Triglyceride Optical Biosensor with Immobilized Lipase Enzymes
Authors: Hasanah Uswatun , Sani Nor Diyana Md , Heng Lee Yook , Idroes Rinaldi , Safitri Eka
Published: November 2019

A novel and simple optical biosensor to detect triglycerides (TGs) has been successfully constructed by using pectin hydrogel membrane as the indicator pH and chromoionophore ETH 5294 (CI), with lipase as the catalyst. The enzymatic working system against TGs releasing H+ ions will affect the color absorbance of CI. The characterization results show that a TG biosensor has the optimum condition and sensitivity at the phosphate buffer concentration of 50 mM, pH 7, and enzyme loading of 60 μg. The biosensor works at the tripalmitin (TP) concentration range of 100–400 mg/dL. With the sensitivity of 0.001 (∆A/(mg/dL)), the biosensor response reaches stability after five minutes, and the limit of detection (LOD) of the TG optical biosensor is 15 mg/dL. Relative standard deviation (RSD) in a reproducibility test was 2.5%, with a 15-day lifespan.
Lost in Classification: Lower Cognitive Functioning in Apparently Cognitive Normal Newly Diagnosed RRMS Patients
Authors: Pitteri Marco , Ziccardi Stefano , Dapor Caterina , Guandalini Maddalena , Calabrese Massimiliano
Published: November 2019

Cognitive functioning in multiple sclerosis (MS) patients is usually related to the classic, dichotomic classification of impaired vs. unimpaired cognition. However, this approach is far from mirroring the real efficiency of cognitive functioning. Applying a different approach in which cognitive functioning is considered as a continuous variable, we aimed at showing that even newly diagnosed relapsing–remitting MS (RRMS) patients might suffer from reduced cognitive functioning with respect to a matched group of neurologically healthy controls (HCs), even if they were classified as having no cognitive impairment (CI). Fifty newly diagnosed RRMS patients and 36 HCs were tested with an extensive battery of neuropsychological tests. By using Z-scores applied to the whole group of RRMS and HCs together, a measure of cognitive functioning (Z-score index) was calculated. Among the 50 RRMS patients tested, 36 were classified as cognitively normal (CN). Even though classified as CN, RRMS patients performed worse than HCs at a global level (p = 0.004) and, more specifically, in the domains of memory (p = 0.005) and executive functioning (p = 0.006). These results highlight that reduced cognitive functioning can be present early in the disease course, even in patients without an evident CI. The current classification criteria of CI in MS should be considered with caution.
The Sonophotocatalytic Degradation of Pharmaceuticals in Water by MnOx-TiO2 Systems with Tuned Band-Gaps
Authors: Khani Zahra , Schieppati Dalma , Bianchi Claudia L. , Boffito Daria C.
Published: November 2019

Advanced oxidation processes (AOPs) are technologies to degrade organic pollutants to carbon dioxide and water with an eco-friendly approach to form reactive hydroxyl radicals. Photocatalysis is an AOP whereby TiO2 is the most adopted photocatalyst. However, TiO2 features a wide (3.2 eV) and fast electron-hole recombination. When Mn is embedded in TiO2, it shifts the absorption wavelength towards the visible region of light, making it active for natural light applications. We present a systematic study of how the textural and optical properties of Mn-doped TiO2 vary with ultrasound applied during synthesis. We varied ultrasound power, pulse length, and power density (by changing the amount of solvent). Ultrasound produced mesoporous MnOx-TiO2 powders with a higher surface area (101–158 m2 g−1), pore volume (0-13–0.29 cc g−1), and smaller particle size (4–10 µm) than those obtained with a conventional sol-gel method (48–129 m2 g−1, 0.14–0.21 cc g−1, 181 µm, respectively). Surprisingly, the catalysts obtained with ultrasound had a content of brookite that was at least 28%, while the traditional sol-gel samples only had 7%. The samples synthesized with ultrasound had a wider distribution of the band-gaps, in the 1.6–1.91 eV range, while traditional ones ranged from 1.72 eV to 1.8 eV. We tested activity in the sonophotocatalytic degradation of two model pollutants (amoxicillin and acetaminophen). The catalysts synthesized with ultrasound were up to 50% more active than the traditional samples.
In-Situ Arc Discharge-Derived FeSn2/Onion-Like Carbon Nanocapsules as Improved Stannide-Based Electrocatalytic Anode Materials for Lithium-Ion Batteries
Authors: Han Dandan , Chatterjee Amrita , Man Long Hin , Or Siu Wing
Published: November 2019

Core/shell-structured FeSn2/onion-like carbon (FeSn2/OLC) nanocapsules of confined size range of sub-50 nm are synthesized via an in-situ arc-discharge process, and are evaluated in comparison with FeSn2 nanoparticles as an improved stannide-based electrocatalytic anode material for Li-ion batteries (LIBs). The in-situ arc-discharge process allows a facile one-pot procedure for forming crystalline FeSn2 stannide alloy nanoparticle cores coated by defective OLC thin shells in addition to a confined crystal growth of the FeSn2 nanoparticle cores. The LIB cells assembled using the FeSn2/OLC nanocapsules as the electrocatalytic anodes exhibit superior full specific discharge capacity of 519 mAh·g−1 and specific discharge capacity retention of ~62.1% after 100 charge-discharge cycles at 50 mA·g−1 specific current. The electrochemical stability of FeSn2/OLC nanocapsules is demonstrated from the good cycle stability of the LIBs with a high specific discharge capacity retention of 67.5% on a drastic change in specific current from 4000 to 50 mA·g−1. A formation mechanism is proposed to describe the confined crystal growth of the FeSn2 nanoparticle cores and the formation of the FeSn2/OLC core/shell structure. The observed electrochemical performance enhancement is ascribed to the synergetic effects of the enabling of a reversible lithiation process during charge-discharge of the LIB cells by the FeSn2 nanoparticle cores as well as the protection of the FeSn2 nanoparticle cores from volume change-induced pulverization and solid electrolyte interphase-induced passivation by the OLC shells.
Metabolic Signature of Hepatic Fibrosis: From Individual Pathways to Systems Biology
Authors: Chang Ming-Ling , Yang Sien-Sing
Published: November 2019

Hepatic fibrosis is a major cause of morbidity and mortality worldwide, as it ultimately leads to cirrhosis, which is estimated to affect up to 2% of the global population. Hepatic fibrosis is confirmed by liver biopsy, and the erroneous nature of this technique necessitates the search for noninvasive alternatives. However, current biomarker algorithms for hepatic fibrosis have many limitations. Given that the liver is the largest organ and a major metabolic hub in the body, probing the metabolic signature of hepatic fibrosis holds promise for the discovery of new markers and therapeutic targets. Regarding individual metabolic pathways, accumulating evidence shows that hepatic fibrosis leads to alterations in carbohydrate metabolism, as aerobic glycolysis is aggravated in activated hepatic stellate cells (HSCs) and the whole fibrotic liver; in amino acid metabolism, as Fischer’s ratio (branched-chain amino acids/aromatic amino acids) decreases in patients with hepatic fibrosis; and in lipid metabolism, as HSCs lose vitamin A-containing lipid droplets during transdifferentiation, and cirrhotic patients have decreased serum lipids. The current review also summarizes recent findings of metabolic alterations relevant to hepatic fibrosis based on systems biology approaches, including transcriptomics, proteomics, and metabolomics in vitro, in animal models and in humans.
Role of ACTN4 in Tumorigenesis, Metastasis, and EMT
Authors: Tentler Dmitri , Lomert Ekaterina , Novitskaya Ksenia , Barlev Nikolai A.
Published: November 2019

The actin-binding protein ACTN4 belongs to a family of actin-binding proteins and is a non-muscle alpha-actinin that has long been associated with cancer development. Numerous clinical studies showed that changes in ACTN4 gene expression are correlated with aggressiveness, invasion, and metastasis in certain tumors. Amplification of the 19q chromosomal region where the gene is located has also been reported. Experimental manipulations with ACTN4 expression further confirmed its involvement in cell proliferation, motility, and epithelial-mesenchymal transition (EMT). However, both clinical and experimental data suggest that the effects of ACTN4 up- or down-regulation may vary a lot between different types of tumors. Functional studies demonstrated its engagement in a number of cytoplasmic and nuclear processes, ranging from cytoskeleton reorganization to regulation of different signaling pathways. Such a variety of functions may be the reason behind cell type and cell line specific responses. Herein, we will review research progress and controversies regarding the prognostic and functional significance of ACTN4 for tumorigenesis.
Sonodynamic Therapy for Gliomas. Perspectives and Prospects of Selective Sonosensitization of Glioma Cells
Authors: Bilmin Krzysztof , Kujawska Tamara , Grieb Paweł
Published: November 2019

Malignant glial tumors (gliomas) are the second (after cerebral stroke) cause of death from diseases of the central nervous system. The current routine therapy, involving a combination of tumor resection, radio-, and chemo-therapy, only modestly improves survival. Sonodynamic therapy (SDT) has been broadly defined as a synergistic effect of sonication applied in combination with substances referred to as “sonosensitizers”. The current review focuses on the possibility of the use of tumor-seeking sonosensitizers, in particular 5-aminolevulinic acid, to control recurring gliomas. In this application, SDT employs a principle similar to that of the more widely-known photodynamic therapy of superficially located cancers, the difference being the use of ultrasound instead of light to deliver the energy necessary to eliminate the sensitized malignant cells. The ability of ultrasound to penetrate brain tissues makes it possible to reach deeply localized intracranial tumors such as gliomas. The major potential advantage of this variant of SDT is its relative non-invasiveness and possibility of repeated application. Until now, there have been no clinical data regarding the efficacy and safety of such treatment for malignant gliomas, but the preclinical data are encouraging.
Mast Cells in Liver Fibrogenesis
Authors: Weiskirchen Ralf , Meurer Steffen K. , Liedtke Christian , Huber Michael
Published: November 2019

Mast cells (MCs) are immune cells of the myeloid lineage that are present in the connective tissue throughout the body and in mucosa tissue. They originate from hematopoietic stem cells in the bone marrow and circulate as MC progenitors in the blood. After migration to various tissues, they differentiate into their mature form, which is characterized by a phenotype containing large granules enriched in a variety of bioactive compounds, including histamine and heparin. These cells can be activated in a receptor-dependent and -independent manner. Particularly, the activation of the high-affinity immunoglobulin E (IgE) receptor, also known as FcεRI, that is expressed on the surface of MCs provoke specific signaling cascades that leads to intracellular calcium influx, activation of different transcription factors, degranulation, and cytokine production. Therefore, MCs modulate many aspects in physiological and pathological conditions, including wound healing, defense against pathogens, immune tolerance, allergy, anaphylaxis, autoimmune defects, inflammation, and infectious and other disorders. In the liver, MCs are mainly associated with connective tissue located in the surrounding of the hepatic arteries, veins, and bile ducts. Recent work has demonstrated a significant increase in MC number during hepatic injury, suggesting an important role of these cells in liver disease and progression. In the present review, we summarize aspects of MC function and mediators in experimental liver injury, their interaction with other hepatic cell types, and their contribution to the pathogenesis of fibrosis.
Electrodeposition of a Ni–P–TiO2/Ti3C2Tx Coating with In Situ Grown Nanoparticles TiO2 on Ti3C2Tx Sheets
Authors: Du Yingchao , Zhang Xiaomeng , Wei Lianqi , Yu Bo , Ma Daqing , Ye Shufeng
Published: November 2019

Protective coatings have received considerable attention for the surface treatment of devices. Herein, in situ grown nanoparticles, TiO2 on Ti3C2Tx sheets (TiO2/Ti3C2Tx), are prepared by a simple hydrothermal oxidation method possessing the layer structure, which is applied to prepare protective coatings. The Ni–P–TiO2/Ti3C2Tx coating is prepared by electroplating technology, revealing more excellent properties than those of the Ni–P coating. Compared with the Ni–P coating, even though the Ni–P–TiO2/Ti3C2Tx coating holds the rough surface, the wettability is changed from hydrophilic to hydrophobic, owing to the gathering existence of TiO2/Ti3C2Tx on the surface and coarse surface texture. In addition, the participation of TiO2/Ti3C2Tx in the Ni–P coating can improve the capacity of corrosion prevention and decrease the corrosion rate. According to the results of hardness and wear tests, microhardness of the Ni–P–TiO2/Ti3C2Tx coating is approximately 1350 kg mm−2 and the coefficient of friction (COF) of Ni–P–TiO2/Ti3C2Tx coatings is about 0.40, which is much lower than that of Ni–P coatings. Thus, the Ni–P–TiO2/Ti3C2Tx coating can be a promising material to protect the surface of equipment.
Otolaryngologists and the Early Diagnosis of Mucopolysaccharidoses: A Cross-Sectional Study
Authors: Torres Danielle de Araujo , Barth Anneliese Lopes , Valente Mariana Pires de Mello , Mello Paulo Pires de , Horovitz Dafne Dain Gandelman
Published: November 2019

Mucopolysaccharidoses (MPS) are a group of inborn errors of metabolism with an aggressive and usually fatal course. Therefore, early treatment is essential because the involvement of head and neck structures is almost always present in MPS. Our study aimed to retrospectively assess—via a chart review and a survey of caregivers—the history of ear, nose and throat (ENT) symptoms, the number of otolaryngology visits prior to diagnosis, and whether otolaryngologists diagnosed the disease in a cohort of MPS patients followed at an academic medical center. Twenty-three patients were evaluated. Age at diagnosis ranged from 0.2 to 33.0 years (median, 3.2 years). Prior to being diagnosed with MPS, 20/23 (87%) patients presented with at least one episode of otalgia, airway disorder, sleep disturbance, speech delay or suspected hearing loss. One patient had an adenotonsillectomy with paracentesis of tympanic membranes. Ten of the 23 patients (43%) were seen by an otolaryngologist before the diagnosis of MPS, none of which had the disease suspected during these visits. Notwithstanding limitations, our results suggest that increased awareness of MPS among otolaryngologists may allow for earlier diagnosis and better management of these patients.
A Fuzzy-Rule-Based PV Inverter Controller to Enhance the Quality of Solar Power Supply: Experimental Test and Validation
Authors: Hannan Mahammad A. , Ghani Zamre A. , Hoque Mohammed M. , Hossain Lipu Molla S.
Published: November 2019

This paper presents the development of fuzzy-based inverter controller for photovoltaic (PV) application to avoid the nonlinearity characteristic and fluctuations of PV inverter output. The fuzzy-based controller algorithm is employed in the PV inverter control system to optimize the duty cycles of the insulated-gate bipolar transistors (IGBTs) and to enhance the inverter outputs with lower harmonic contents and unity power factor. The developed fuzzy-based PV inverter controller is implemented in the MATLAB/Simulink models and experimentally tested in a dSPACE DS1104 process controller. The obtained simulation result of the developed fuzzy-based PV inverter controller is validated with experimental results under different performance conditions. It is seen that the experimental results of the switching signals, inverter voltage and current, control parameters, and total harmonic distortion (THD) of load current and output voltage of the PV inverter are closely matched with that of the simulation results. To validate the inverter performance, the proposed fuzzy-based PV inverter controller outperforms other studies with a voltage THD of 2.5% and a current THD of 3.5% with unity power factor.
A 0.5~0.7 V LC Digitally Controlled Oscillator Based on a Multi-Stage Capacitance Shrinking Technique
Authors: Wang Zixuan , Wu Hongyang , Wang Xin , Shi Mingmin , Hu Shanwen , Guo Yufeng , Cai Zhikuang
Published: November 2019

This paper presents a 2.4 GHz LC digitally controlled oscillator (DCO) at near-threshold supplies (0.5~0.7 V). It was a challenge to achieve a low voltage, low power, and high resolution simultaneously. DCOs with metal oxide semiconductor (MOS) varactors consume low power, but their resolution is limited. ΔΣ-DCOs can achieve a high resolution at the cost of high power consumption. A multi-stage capacitance shrinking technique was proposed in this paper to address the tradeoff mentioned above. The unit variable capacitance of the LC tank was largely reduced by the bridging capacitors and the number of stages. A current-reuse technique was used to further lower the power. Based on the above techniques, the prototype was fabricated using a 130-nm complementary MOS (CMOS) technology with multiple supplies (0.5~0.7 V for the DCO core, 1.2 V for the buffer). The measurement results showed that the phase noise at a 0.6-V supply was −126.27 dBc/Hz at 1 MHz and −125.9480 dBc/Hz at 1 MHz at the carriers of 2.4 GHz and 2.5 GHz, respectively. The best figure of merit (FoM) of 195.68 was obtained when VDD = 0.6 V. The DCO core consumed 1.1 mA at a 0.6-V supply.
Motion Planning of a Second-Order Nonholonomic Chained Form System Based on Holonomy Extraction
Authors: Ito Masahide
Published: November 2019

This paper proposes a motion planning algorithm for dynamic nonholonomic systems represented in a second-order chained form. The proposed approach focuses on the so-called holonomy resulting from a kind of motion that traverses a closed path in a reduced configuration space of the system. According to the author’s literature survey, control approaches that make explicit use of holonomy exist for kinematic nonholonomic systems but does not exist for dynamic nonholonomic systems. However, the second-order chained form system is controllable. Also, the structure of the second-order chained form system analogizes with the one of the first-order chained form for kinematic nonholonomic systems. These survey and perspectives brought a hypothesis that there exists a specific control strategy for extracting holonomy of the second-order chained form system to the author. To verify this hypothesis, this paper shows that the holonomy of the second-order chained form system can be extracted by combining two appropriate pairs of sinusoidal inputs. Then, based on such holonomy extraction, a motion planning algorithm is constructed. Furthermore, the effectiveness is demonstrated through some simulations including an application to an underactuated manipulator.
Effect of the High-Temperature Off-State Stresses on the Degradation of AlGaN/GaN HEMTs
Authors: Lin Jinfu , Liu Hongxia , Wang Shulong , Liu Chang , Li Mengyu , Wu Lei
Published: November 2019

GaN-based high electron mobility transistors offer high carrier density combined with high electron mobility and often require operation at high frequencies, voltages, and temperatures. The device may be under high temperature and high voltage at the same time in actual operation. In this work, the impact of separate off-state stresses, separate high-temperature stresses, and off-state stresses at high temperatures on AlGaN/GaN high electron mobility transistors (HEMTs) grown on Si substrates was investigated. The output current and gate leakage of the device degenerated to different degrees under either isolated off-state or high-temperature stress. The threshold voltage of the device only exhibited obvious negative drift under the action of high-temperature and off-state stresses. The parameter at high temperature (or room temperature) before stress application was the reference. We found that there was no significant difference in the degradation rate of drain current and transconductance peak when the same off-state stress was applied to the device at different temperatures. It was concluded that, under the high-temperature off-state electric field pressure, there were two degradation mechanisms: one was the inverse piezoelectric polarization mechanism only related to the electric field, and the other was the degradation mechanism of the simultaneous action of temperature and electric field.
Post-Fire Management Impact on Natural Forest Regeneration through Altered Microsite Conditions
Authors: Marcolin Enrico , Marzano Raffaella , Vitali Alessandro , Garbarino Matteo , Lingua Emanuele
Published: November 2019

High severity stand-replacing wildfires can deeply affect forest ecosystems whose composition includes plant species lacking fire-related traits and specific adaptations. Land managers and policymakers need to be aware of the importance of properly managing these ecosystems, adopting post-disturbance interventions designed to reach management goals, and restoring the required ecosystem services. Recent research frequently found that post-fire salvage logging negatively affects natural regeneration dynamics, thereby altering successional pathways due to a detrimental interaction with the preceding disturbance. In this study, we compared the effects of salvage logging and other post-disturbance interventions (adopting different deadwood management strategies) to test their impact on microclimatic conditions, which potentially affect tree regeneration establishment and survival. After one of the largest and most severe wildfires in the Western Alps that affected stand-replacing behavior (100% tree mortality), a mountain forest dominated by Pinus sylvestris L., three post-fire interventions were adopted (SL-Salvage Logging, logging of all snags; CR-Cut and Release, cutting snags and releasing all deadwood on the ground; NI-No Intervention, all snags left standing). The differences among interventions concerning microclimatic conditions (albedo, surface roughness, solar radiation, soil moisture, soil temperature) were analyzed at different spatial scales (site, microsite). The management interventions influenced the presence and density of safe sites for regeneration. Salvage logging contributed to the harsh post-fire microsite environment by increasing soil temperature and reducing soil moisture. The presence of deadwood, instead, played a facilitative role in ameliorating microclimatic conditions for seedlings. The CR intervention had the highest soil moisture and the lowest soil temperature, which could be crucial for seedling survival in the first post-fire years. Due to its negative impact on microclimatic conditions affecting the availability of preferential microsites for regeneration recruitment, salvage logging should not be considered as the only intervention to be applied in post-fire environments. In the absence of threats or hazards requiring specific management actions (e.g., public safety, physical hazards for facilities), in the investigated ecosystems, no intervention, leaving all deadwood on site, could result in better microclimatic conditions for seedling establishment. A preferred strategy to speed-up natural processes and further increase safe sites for regeneration could be felling standing dead trees whilst releasing deadwood (at least partially) on the ground.
High Oxygen Modified Atmosphere Packaging Negatively Influences Consumer Acceptability Traits of Pork
Authors: Peng Yunling , Adhiputra Karunia , Padayachee Anneline , Channon Heather , Ha Minh , Warner Robyn Dorothy
Published: November 2019

Current trends in meat packaging have seen a shift from conventional overwrap to vacuum packing (VAC) and modified atmosphere packaging (MAP). The aim of this experiment was to investigate the effects of high oxygen MAP (HiOxMAP) of pork loins compared with vacuum packed (VAC) on eating quality and colour, after storage in simulated illuminated retail display conditions. Pork loins (n = 40) were cut and stored under two packaging methods (HiOxMAP, 80% O2, 20% CO2; VAC) for up to 14 days, with samples taken at various times for measurements. After 7 days of storage, HiOxMAP samples exhibited inferior consumer acceptability for tenderness, flavor, overall liking, quality and re-purchase intention as well as higher shear force and hardness, relative to VAC samples (p < 0.05 for all). Loins stored in HiOxMAP had higher lightness (L*), redness (a*) and yellowness (b*) values at 3 and 7 days, but lower ratio of oxymoglobin to metmyoglobin (oxy/met) values in the meat surface at 14 days of display, relative to VAC samples (p < 0.05 for all). The oxy/met ratio declined from 2.3 to 1.7 between days 3 and 14 of display in HiOxMAP samples (p < 0.05), whereas the ratio was similar and stayed relatively high for VAC samples. VAC samples produced consistently higher colour values (a*, b*, oxy/met) when left to bloom 30 min after removal from packaging (p < 0.05). Lipid oxidation values, measured using thiobarbituric acid reactive substances, in HiOxMAP pork loins, were higher at all time points compared to VAC during the 14 day storage period (p < 0.05). The use of vacuum packing for retail shelves, should be considered as the preferred option, over HiOxMAP.
Variation in Pea (Pisum sativum L.) Seed Quality Traits Defined by Physicochemical Functional Properties
Authors: Santos Carla S. , Carbas Bruna , Castanho Ana , Vasconcelos Marta W. , Vaz Patto Maria Carlota , Domoney Claire , Brites Carla
Published: November 2019

Pea is one of the most produced and consumed pulse crops around the world. The study of genetic variability within pea germplasm is an important tool to identify outstanding accessions with optimal functional and nutritional qualities. In the present study, a collection of 105 pea accessions was analysed for physicochemical properties, pasting viscosity, and basic composition parameters. While pasting viscosities were negatively correlated to hydration capacity, cooking time, and basic composition, a positive correlation was found between the hydration capacity and the basic composition parameters. Basic composition (protein, fibre, fat, and resistant starch) parameters were further evaluated regarding seed trait morphology, namely, seed shape, colour, and surface. Allelic characterisation at the r and rb genetic loci was performed in a subgroup of 32 accessions (3 phenotyped as smooth and 29 as rough seeded), revealing that none of the initially classified rough-seeded accessions were rb mutants, 19 were r mutants, and 13 were neither r nor rb. Despite their initial phenotypic classification, the 13 accessions genetically classified as smooth behaved differently (p < 0.05) to the 19 r mutants in terms of physicochemical properties, pasting viscosity, and basic composition parameters. Using multivariate analysis of the most discriminatory parameters for the food-related traits studied, the best-performing accessions at functional and nutritional levels were identified for future plant breeding to improve field pea production and consumption.
A Weighted Genomic Relationship Matrix Based on Fixation Index (FST) Prioritized SNPs for Genomic Selection
Authors: Chang Ling-Yun , Toghiani Sajjad , Hay El Hamidi , Aggrey Samuel E. , Rekaya Romdhane
Published: November 2019

A dramatic increase in the density of marker panels has been expected to increase the accuracy of genomic selection (GS), unfortunately, little to no improvement has been observed. By including all variants in the association model, the dimensionality of the problem should be dramatically increased, and it could undoubtedly reduce the statistical power. Using all Single nucleotide polymorphisms (SNPs) to compute the genomic relationship matrix (G) does not necessarily increase accuracy as the additive relationships can be accurately estimated using a much smaller number of markers. Due to these limitations, variant prioritization has become a necessity to improve accuracy. The fixation index (FST) as a measure of population differentiation has been used to identify genome segments and variants under selection pressure. Using prioritized variants has increased the accuracy of GS. Additionally, FST can be used to weight the relative contribution of prioritized SNPs in computing G. In this study, relative weights based on FST scores were developed and incorporated into the calculation of G and their impact on the estimation of variance components and accuracy was assessed. The results showed that prioritizing SNPs based on their FST scores resulted in an increase in the genetic similarity between training and validation animals and improved the accuracy of GS by more than 5%.
Morphogenesis of New Straits and Islands Originated in the European Arctic Since the 1980s
Authors: Ziaja Wieslaw , Ostafin Krzysztof
Published: November 2019

Several new islands and many islets have appeared in the European Arctic since the end of the 20th century due to glacial recession under climate warming. The specificity of the formation of each individual strait and island is shown in the paper (apart from its location and timing of its origin). Analysis of available maps and satellite images of all three European Arctic archipelagos, from different times since 1909–1910, was the main research method. There are three pathways of the morphogenesis of the new islands: (1) simultaneous recession of glaciers from both sides of a depression in bedrock being a potential strait (typical in Franz Josef Land), (2) uncovering a rocky hill (which protrudes from a depression in bedrock) from under a receding glacier, (3) recession of one glacier which had reached a rocky fragment of a coastline (e.g., headland or peninsula), being a potential new island, during a maximum extent of this glacier during the Little Ice Age (in the beginning of the 20th century). Additional straits and islands are currently at the stage of formation and will continue to form in the European Arctic in the case of further warming or stabilization of the current climate conditions.
Establishing and Sustaining a Culture of Evidence-Based Practice: An Evaluation of Barriers and Facilitators to Implementing the Best Practice Spotlight Organization Program in the Australian Healthcare Context
Authors: Sharplin Greg , Adelson Pam , Kennedy Kate , Williams Nicola , Hewlett Roslyn , Wood Jackie , Bonner Rob , Dabars Elizabeth , Eckert Marion
Published: November 2019

Background: Nurses and midwives are central to the implementation and delivery of quality care through evidence-based practice (EBP). However, implementation of EBP in nursing and midwifery is under-researched with few examples of systematic and sustained change. The Registered Nurses Association of Ontario’s Best-Practice Spotlight Organization (BPSO) Program was adopted in South Australia as a framework to systematically implement EBP in two diverse and complex healthcare settings. Methods: The study was a post-implementation, mixed-method evaluation conducted at two healthcare settings in Adelaide, South Australia utilizing qualitative and quantitative data. Proctor’s implementation evaluation framework guided the evaluation design. Information sources included; interviews, focus groups, questionnaires, and document review. Results: Clinical and executive staff (n = 109 participants) from a broad range of stakeholder groups participated in the interviews, focus groups, and returned questionnaires. A number of facilitators directly affecting program implementation were identified; these pertained to embedding continuity into the program’s implementation and delivery, a robust governance structure, and executive sponsorship. Barriers to implementation were also identified. These barriers pertained to organizational or workforce challenges; staff turnover and movement (e.g., secondment), insufficient staff to allow people to attend training, and a lack of organizational commitment to the program, especially at an executive level. As a result of successful implementation, it was observed that over three years, the BPSO program positively influenced the uptake and implementation of EBP by clinicians and the organizations into which they were introduced. Conclusions: The BPSO model can be translocated to new healthcare systems and has the potential to act as a mechanism for establishing and sustaining EBP change. This study was the first to apply an implementation evaluation framework to the BPSO program, which allowed for structured analysis of facilitating or impeding factors that affected implementation success. The findings have important implications for other health systems looking to translocate the same or similar EBP programs, as well as contributing to the growing body of implementation evaluation literature.
Lessons from the Trials for the Desirable Effects of Sodium Glucose Co-Transporter 2 Inhibitors on Diabetic Cardiovascular Events and Renal Dysfunction
Authors: Wakisaka Masanori , Kamouchi Masahiro , Kitazono Takanari
Published: November 2019

Recent large placebo-controlled trials of sodium glucose co-transporter 2 (SGLT2) inhibitors revealed desirable effects on heart failure (HF) and renal dysfunction; however, the mechanisms underlying these effects are unknown. The characteristic changes in the early stage of diabetic cardiomyopathy (DCM) are myocardial and interstitial fibrosis, resulting in diastolic and subsequent systolic dysfunction, which leads to clinical HF. Pericytes are considered to play crucial roles in myocardial and interstitial fibrosis. In both DCM and diabetic retinopathy (DR), microaneurysm formation and a decrease in capillaries occur, triggered by pericyte loss. Furthermore, tubulointerstitial fibrosis develops in early diabetic nephropathy (DN), in which pericytes and mesangial cells are thought to play important roles. Previous reports indicate that pericytes and mesangial cells play key roles in the pathogenesis of DCM, DR and DN. SGLT2 is reported to be functionally expressed in pericytes and mesangial cells, and excessive glucose and Na+ entry through SGLT2 causes cellular dysfunction in a diabetic state. Since SGLT2 inhibitors can attenuate the high glucose-induced dysfunction of pericytes and mesangial cells, the desirable effects of SGLT2 inhibitors on HF and renal dysfunction might be explained by their direct actions on these cells in the heart and kidney microvasculature.
Genome-Wide Correlation of 36 Agronomic Traits in the 287 Pepper (Capsicum) Accessions Obtained from the SLAF-seq-Based GWAS
Authors: Wu Lang , Wang Peng , Wang Yihao , Cheng Qing , Lu Qiaohua , Liu Jinqiu , Li Ting , Ai Yixin , Yang Wencai , Sun Liang , Shen Huolin
Published: November 2019

There are many agronomic traits of pepper (Capsicum L.) with abundant phenotypes that can benefit pepper growth. Using specific-locus amplified fragment sequencing (SLAF-seq), a genome-wide association study (GWAS) of 36 agronomic traits was carried out for 287 representative pepper accessions. To ensure the accuracy and reliability of the GWAS results, we analyzed the genetic diversity, distribution of labels (SLAF tags and single nucleotide polymorphisms (SNPs)) and population differentiation and determined the optimal statistical model. In our study, 1487 SNPs were highly significantly associated with 26 agronomic traits, and 2126 candidate genes were detected in the 100-kb region up- and down-stream near these SNPs. Furthermore, 13 major association peaks were identified for 11 key agronomic traits. Then we examined the correlations among the 36 agronomic traits and analyzed SNP distribution and found 37 SNP polymerization regions (total size: 264.69 Mbp) that could be selected areas in pepper breeding. We found that the stronger the correlation between the two traits, the greater the possibility of them being in more than one polymerization region, suggesting that they may be linked or that one pleiotropic gene controls them. These results provide a theoretical foundation for future multi-trait pyramid breeding of pepper. Finally, we found that the GWAS signals were highly consistent with those from the nuclear restorer-of-fertility (Rf) gene for cytoplasmic male sterility (CMS), verifying their reliability. We further identified Capana06g002967 and Capana06g002969 as Rf candidate genes by functional annotation and expression analysis, which provided a reference for the study of cytoplasmic male sterility in Capsicum.
A Novel System for Spinal Muscular Atrophy Screening in Newborns: Japanese Pilot Study
Authors: Shinohara Masakazu , Niba Emma Tabe Eko , Wijaya Yogik Onky Silvana , Takayama Izumi , Mitsuishi Chisako , Kumasaka Sakae , Kondo Yoichi , Takatera Akihiro , Hokuto Isamu , Morioka Ichiro , Ogiwara Kazutaka , Tobita Kimimasa , Takeuchi Atsuko , Nishio Hisahide ,   for the SMA-NBS PILOT STUDY GROUP
Published: November 2019

Spinal muscular atrophy (SMA) is a neuromuscular disorder caused by SMN1 gene deletion/mutation. The drug nusinersen modifies SMN2 mRNA splicing, increasing the production of the full-length SMN protein. Recent studies have demonstrated the beneficial effects of nusinersen in patients with SMA, particularly when treated in early infancy. Because nusinersen treatment can alter disease trajectory, there is a strong rationale for newborn screening. In the current study, we validated the accuracy of a new system for detecting SMN1 deletion (Japanese patent application No. 2017-196967, PCT/JP2018/37732) using dried blood spots (DBS) from 50 patients with genetically confirmed SMA and 50 controls. Our system consists of two steps: (1) targeted pre-amplification of SMN genes by direct polymerase chain reaction (PCR) and (2) detection of SMN1 deletion by real-time modified competitive oligonucleotide priming-PCR (mCOP-PCR) using the pre-amplified products. Compared with PCR analysis results of freshly collected blood samples, our system exhibited a sensitivity of 1.00 (95% confidence interval [CI] 0.96–1.00) and a specificity of 1.00 (95% CI 0.96–1.00). We also conducted a prospective SMA screening study using DBS from 4157 Japanese newborns. All DBS tested negative, and there were no screening failures. Our results indicate that the new system can be reliably used in SMA newborn screening.
Acute Effects of Immersive Virtual Reality Exercise on Young Adults’ Situational Motivation
Authors: Liu Wenxi , Zeng Nan , Pope Zachary C. , McDonough Daniel J. , Gao Zan
Published: November 2019

The development of innovative technology, such as virtual reality (VR), has provided opportunities for promoting physical activity (PA) in a fun and engaging manner. The purpose of this study was to examine differences in young adults’ situational motivation (SM) among immersive VR, non-immersive VR, and traditional stationary cycling sessions. In all, 49 healthy college students (35 females; Mage = 23.6 years, SD = 3.4; M%BF = 24.0%, SD = 7.5) completed three separate 20 min cycling sessions: (1) immersive VR cycling; (2) non-immersive VR cycling; and (3) traditional cycling. Participants’ SM was assessed via the situational motivation scale, which included four subconstructs: intrinsic motivation, identified regulation, external regulation, and amotivation. Repeated measures ANOVAs indicated significant differences for situational motivation between cycling sessions (F (2, 96) = 4.74–53.04, p < 0.01, ηp2 = 0.090–0.525). Specifically, participants elicited the highest level of intrinsic motivation in immersive VR cycling compared to the other two sessions. Moreover, participants in both immersive VR and traditional cycling showed greater identified regulation than the non-immersive VR session. Furthermore, participants showed greater external regulation compared to the immersive VR session. In addition, greater amotivation was observed in non-immersive VR compared to the immersive VR session. Findings suggested that immersive VR exercise has the potential to be an attractive exercise alternative, possibly promoting greater PA participation and adherence among young adults.