bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of Public Health Indicators to Assess Individual Happiness in Post-Disaster Recovery
Authors: Sun Yingying , Yan Tingting
Published: October 2019

Purpose: Very few studies have examined the influential factors of survivors’ feelings of happiness in the context of nuclear accidents. This paper aims to fill this gap with reference to the recovery process in Fukushima City following the 2011 Tohoku Earthquake Tsunami in Japan. Methods: Open access data were sourced from the 2015 Social Survey on Living and Disaster Recovery (SSLDR) (N = 1439) of Fukushima citizens. Pearson’s Chi-square Test and the t-test were employed to examine gender differences with regard to happiness and exploratory variables. Following this, a multiple linear regression analysis was conducted to investigate the determinants of happiness. Results: The results showed that, compared to females, male respondents were unhappier and reported more property loss and less neighborhood connectedness. Individuals’ mental and physical health and neighborhood connectedness were found to be significantly correlated with their happiness. However, the disaster-related variables of people’s evaluation of recovery achievement, concerns around the health impacts of radiation, property loss in the disaster, and experiences of casualty, had no effects on happiness. Conclusion: These findings indicate that policies and countermeasures dealing with disaster recovery over the long term should continuously focus on health issues and social relationships.
Comparison between the Physiological Responses and Subjective Ratings of a Group of Male Students to Three Backpack Designs
Authors: Ramadan Mohamed Z. , Al-Tayyar Sultan N.
Published: October 2019

It is important for schoolchildren and their parents (or guardians) to know which backpacks exert the least strain on the cardiorespiratory system. In this study, we investigated the physiological responses of participants while they were walking on a treadmill and wearing one of three different backpacks (A, B, and C) under two different load-carrying conditions (equivalent to 10% and 15% of their body mass, respectively). The first condition was used as a control and involved walking without a backpack, while the second involved wearing a backpack and carrying a certain weight. Thirty-one male students from King Saud University walked on a treadmill at 0.861 m/s and at a 0% inclination angle, while having their heart rates (beatsmin−1), oxygen uptakes (VO2, mLmin−1), respiratory rates (breaths, VO2min−1), perceived exertion rates (PER, Borg scale), and backpack preference rates (BPR) measured and recorded. The results of our within-subject experimental design revealed that the physiological results varied significantly depending on the type of backpack. Backpacks B and C were superior to Backpack A, resulting in lower physiological responses and higher subjective preferences. Students carrying more weight experienced higher physiological stress; moreover, the use of Backpack C led to the lowest physiological strains and higher subjective preferences.
Evaluation of the Burdening on the Czech Population by Brominated Flame Retardants
Authors: Logerová Hana , Tůma Petr , Stupák Michal , Pulkrábová Jana , Dlouhý Pavel
Published: October 2019

The completed environmental study was concerned with assessing the exposure of the Czech population to polybrominated diphenyl ethers (PBDEs). Simultaneously, the levels of polychlorinated pollutants such as polychlorinated biphenyls (PCBs) and chlorinated diphenyl ethanes (DDTs) were also monitored. The pollutant levels were newly measured in solid fat tissue removed during plastic surgery. A total of 107 samples of fat were taken from 19–76-year-old volunteers. A total of 16 PBDE congeners were determined, of which only six occur in more than 38% of fat tissue samples. The total PBDE level attains an average value of 3.31 ng/g, which is 25% less than was measured in 2009. On the other hand, there was an increase in the levels of two PCB congeners, which was caused by an increase of the total PCB concentration from level of 625.5 ng/g, published in 2009, to the current level of 776 ng/g. The level of DDTs decreased and currently has a value of 467.4 ng/g, which is about 24% lower than in 2009. The contamination of obese middle-aged women in Czechia by more modern types of pollutants, such as PBDEs, is incomparably lower than that by PCBs and DDTs and is also decreasing in time.
Sex Differences in the Relationship between Student School Burnout and Problematic Internet Use among Adolescents
Authors: Tomaszek Katarzyna , Muchacka-Cymerman Agnieszka
Published: October 2019

Background: The Internet has many positive sides, but it can also have a negative impact on human emotional-cognitive and behavioral functioning, especially during adolescence. To the most common consequences, the authors add addiction of the teenager to the Internet. This addiction is related to many negative physical and mental problems, including depression, substance abuse and social isolation. Methods: In the study, SSBS (Student School Burnout Scale) was used to measure the level of burnout, and the level of Internet addiction was measured using PUI (Internet Addiction Test). The research was carried out among 230 individuals aged 17–20 years. Results: The results of the research showed that higher level of school burnout is related to higher Internet addiction indicators, and connection was stronger in the male group. Gender significantly predicted Internet addiction and moderated the link between school burnout and Internet addiction. Hierarchical multiple regression analyses confirmed different predictors of Internet addiction for male and female students. However, for both groups, higher burnout due to parents was a significant predictor of Internet addiction (IA). Discussion: Internet addiction was predicted by school burnout, appearing as a result of parental pressure for high school achievements. In addition, school burnout and school-related characteristics have greater prediction power of Internet addiction and its indicators in a male group.
The Occurrence and Risks of Selected Emerging Pollutants in Drinking Water Source Areas in Henan, China
Authors: Wu Donghai , Zhou Ying , Lu Guanghua , Hu Kai , Yao Jingjing , Shen Xinghou , Wei Lei
Published: October 2019

The occurrence of organic micropollutants (OMPs) in aqueous environments has potential effects on ecological safety and human health. Three kinds of OMPs (namely, pharmaceuticals, ultraviolet (UV) filters and organophosphate esters (OPEs)) in four drinking water source areas in Henan Province of China were analyzed, and their potential risks were evaluated. Among 48 target chemicals, 37 pollutants with total concentrations ranging from 403.0 to 1751.6 ng/L were detected in water, and 13 contaminants with total concentrations from 326.0 to 1465.4 ng/g (dry weight) were observed in sediment. The aqueous pollution levels in Jiangang Reservoir and Shahe Water Source Area were higher than that in Nanwan Reservoir and Baiguishan Reservoir, while the highest total amount of pollutants in sediment was found in Baiguishan Reservoir. Compared with pharmaceuticals and UV filters, OPEs presented higher concentrations in all investigated drinking water source areas. The highest observed concentration was triphenylphosphine oxide (TPPO, 865.2 ng/L) in water and tripentyl phosphate (TPeP, 1289.8 ng/g) in sediment. Moreover, the risk quotient (RQ) analysis implies that the determined aqueous contaminants exhibited high risks to algae and invertebrates, whereas moderate risk to fish was exhibited. The health risk assessment of aqueous OMPs by means of the hazard index (HI) indicates that the risks to adults and children were negligible. These observations are expected to provide useful information for the assessment of water quality in drinking water sources in Henan, China.
The Prevalence of Vaping and Smoking as Modes of Delivery for Nicotine and Cannabis among Youth in Canada, England and the United States
Authors: Fataar Fathima , Hammond David
Published: October 2019

Background: Vaping has become an increasingly common mode of administration for both nicotine and cannabis, with overlap among users, devices, as well as nicotine and cannabis companies. There is a need to understand patterns of use among youth, including the way nicotine and cannabis are administered. Methods: Data are from Wave 2 of the ITC Youth Tobacco and Vaping survey, an online survey conducted in 2018 among 16–19 year-olds recruited from commercial panels in Canada (n = 3757), England (n = 3819), and the U.S. (n = 3961). The prevalence of past 30-day vaping nicotine, non-nicotine and cannabis substances, as well as cannabis modes of use was examined. Logistic regression models examined between country differences in prevalence. Results: Past 30-day cannabis use was highest among Canadian youth (16.6%), followed by youth in the U.S. (13.8%) and England (9.0%). Vaping e-cigarettes was substantially more prevalent than vaping cannabis in all three countries. All forms of cannabis use were higher among Canadian and U.S. youth compared to England (p < 0.001 for all). Past 30-day cannabis users in the U.S. were more likely to report vaping cannabis oil (30.1%), and consuming solid concentrates such as wax and shatter (30.2%), compared to cannabis users in Canada (18.6% and 22.9%) and England (14.3% and 11.0%; p < 0.001 for all). Conclusions: Youth are administering cannabis and nicotine using a wide diversity of modes. Cannabis users in the U.S.—where an increasing number of states have legalized medical and non-medical cannabis—reported notably higher use of more potent cannabis products, including cannabis oils and extracts.
Urban Air Pollution Particulates Suppress Human T-Cell Responses to Mycobacterium Tuberculosis
Authors: Ibironke Olufunmilola , Carranza Claudia , Sarkar Srijata , Torres Martha , Choi Hyejeong Theresa , Nwoko Joyce , Black Kathleen , Quintana-Belmares Raul , Osornio-Vargas Álvaro , Ohman-Strickland Pamela , Schwander Stephan
Published: October 2019

Tuberculosis (TB) and air pollution both contribute significantly to the global burden of disease. Epidemiological studies show that exposure to household and urban air pollution increase the risk of new infections with Mycobacterium tuberculosis (M.tb) and the development of TB in persons infected with M.tb and alter treatment outcomes. There is increasing evidence that particulate matter (PM) exposure weakens protective antimycobacterial host immunity. Mechanisms by which exposure to urban PM may adversely affect M.tb-specific human T cell functions have not been studied. We, therefore, explored the effects of urban air pollution PM2.5 (aerodynamic diameters ≤2.5µm) on M.tb-specific T cell functions in human peripheral blood mononuclear cells (PBMC). PM2.5 exposure decreased the capacity of PBMC to control the growth of M.tb and the M.tb-induced expression of CD69, an early surface activation marker expressed on CD3+ T cells. PM2.5 exposure also decreased the production of IFN-γ in CD3+, TNF-α in CD3+ and CD14+ M.tb-infected PBMC, and the M.tb-induced expression of T-box transcription factor TBX21 (T-bet). In contrast, PM2.5 exposure increased the expression of anti-inflammatory cytokine IL-10 in CD3+ and CD14+ PBMC. Taken together, PM2.5 exposure of PBMC prior to infection with M.tb impairs critical antimycobacterial T cell immune functions.
Evaluation and Selection of HazMat Transportation Alternatives: A PHFLTS- and TOPSIS-Integrated Multi-Perspective Approach
Authors: Chen Zhen-Song , Li Min , Kong Wen-Tao , Chin Kwai-Sang
Published: October 2019

Outsourcing the hazardous materials (HazMat) transportation is an effective way for manufacturing enterprises to avoid risks and accidents as well as to retain sustainable development in economic growth and social inclusion while not bringing negative impacts on the public and the environment. It is imperative to develop viable and effective approaches to selecting the most appropriate HazMat transportation alternatives. This paper aims at proposing an integrated multi-criteria group decision making approach that combines proportional hesitant fuzzy linguistic term set (PHFLTS) and the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) to address the problem of HazMat transportation alternative evaluation and selection. PHFLTSs are adopted to represent the congregated individual evaluations in a bid to avoid information loss and increase the reliability of results. Two weight assignment models are then proposed to determine the comprehensive weights of experts and criteria. Furthermore, several novel manipulations of PHFLTS are also defined to enrich its applicability. The TOPSIS method is subsequently extended to the context of PHFLTSs to rank alternatives and choose the best one. Eventually, the feasibility and validity of the proposed approach are verified by a practical case study of a HazMat transportation alternative evaluation and selection decision and further comparison analyses.
A Drift-of-Stay Pattern Extraction Method for Indoor Pedestrian Trajectories for the Error and Accuracy Assessment of Indoor Wi-Fi Positioning
Authors: Yu Dengbo , Hu Qingwu , Wang Shaohua
Published: October 2019

The uncertainty of indoor Wi-Fi positioning is susceptible to many factors, such as sensor distribution, the internal environment (e.g., of a shopping mall), differences between receivers, and the flow of people. In this paper, an indoor pedestrian trajectory pattern mining approach for the assessment of the error and accuracy of indoor Wi-Fi positioning is proposed. First, the stay points of the customer were extracted from the pedestrian trajectories based on the spatiotemporal staying patterns of the customers in a shopping mall. Second, the drift points were distinguished from the stay points through analysis of noncustomer behavior patterns. Finally, the drift points were presented to calculate the errors in the pedestrian trajectories for the accuracy assessment of the indoor Wi-Fi positioning system. A one-month indoor pedestrian trajectories dataset from the Xinxiang Baolong shopping mall in Henan Province, China, was used for the assessment of the error and accuracy values with the proposed approach. The experimental results were verified by incorporating the distribution of the AP sensors. The proposed approach using big data pattern mining can explore the error distribution of indoor positioning systems, which can provide strong support for improving indoor positioning accuracy in the future.
An Intersection-First Approach for Road Network Generation from Crowd-Sourced Vehicle Trajectories
Authors: Zhang Caili , Xiang Longgang , Li Siyu , Wang Dehao
Published: October 2019

Extracting highly detailed and accurate road network information from crowd-sourced vehicle trajectory data, which has the advantages of being low cost and able to update fast, is a hot topic. With the rapid development of wireless transmission technology, spatial positioning technology, and the improvement of software and hardware computing ability, more and more researchers are focusing on the analysis of Global Positioning System (GPS) trajectories and the extraction of road information. Road intersections are an important component of roads, as they play a significant role in navigation and urban planning. Even though there have been many studies on this subject, it remains challenging to determine road intersections, especially for crowd-sourced vehicle trajectory data with lower accuracy, lower sampling frequency, and uneven distribution. Therefore, we provided a new intersection-first approach for road network generation based on low-frequency taxi trajectories. Firstly, road intersections from vector space and raster space were extracted respectively via using different methods; then, we presented an integrated identification strategy to fuse the intersection extraction results from different schemes to overcome the sparseness of vehicle trajectory sampling and its uneven distribution; finally, we adjusted road information, repaired fractured segments, and extracted the single/double direction information and the turning relationships of the road network based on the intersection results, to guarantee precise geometry and correct topology for the road networks. Compared with other methods, this method shows better results, both in terms of their visual inspections and quantitative comparisons. This approach can solve the problems mentioned above and ensure the integrity and accuracy of road intersections and road networks. Therefore, the proposed method provides a promising solution for enriching and updating navigable road networks and can be applied in intelligent transportation systems.
GEOBIA Achievements and Spatial Opportunities in the Era of Big Earth Observation Data
Authors: Lang Stefan , Hay Geoffrey , Baraldi Andrea , Tiede Dirk , Blaschke Thomas
Published: October 2019

The primary goal of collecting Earth observation (EO) imagery is to map, analyze, and contribute to an understanding of the status and dynamics of geographic phenomena. In geographic information science (GIScience), the term object-based image analysis (OBIA) was tentatively introduced in 2006. When it was re-formulated in 2008 as geographic object-based image analysis (GEOBIA), the primary focus was on integrating multiscale EO data with GIScience and computer vision (CV) solutions to cope with the increasing spatial and temporal resolution of EO imagery. Building on recent trends in the context of big EO data analytics as well as major achievements in CV, the objective of this article is to review the role of spatial concepts in the understanding of image objects as the primary analytical units in semantic EO image analysis, and to identify opportunities where GEOBIA may support multi-source remote sensing analysis in the era of big EO data analytics. We (re-)emphasize the spatial paradigm as a key requisite for an image understanding system capable to deal with and exploit the massive data streams we are currently facing; a system which encompasses a combined physical and statistical model-based inference engine, a well-structured CV system design based on a convergence of spatial and colour evidence, semantic content-based image retrieval capacities, and the full integration of spatio-temporal aspects of the studied geographical phenomena.
Tree-Based and Optimum Cut-Based Origin-Destination Flow Clustering
Authors: Xiang Qiuliang , Wu Qunyong
Published: October 2019

Data about the movements of diverse objects, including human beings, animals, and commodities, are collected in growing amounts as location-aware technologies become pervasive. Clustering has become an increasingly important analytical tool for revealing travel patterns from large-scale movement datasets. Most existing methods for origin-destination (OD) flow clustering focus on the geographic properties of an OD flow but ignore the temporal information preserved in the OD flow, which reflects the dynamic changes in the travel patterns over time. In addition, most methods require some predetermined parameters as inputs and are difficult to adjust considering the changes in the users’ demands. To overcome such limitations, we present a novel OD flow clustering method, namely, TOCOFC (Tree-based and Optimum Cut-based Origin-Destination Flow Clustering). A similarity measurement method is proposed to quantify the spatial similarity relationship between OD flows, and it can be extended to measure the spatiotemporal similarity between OD flows. By constructing a maximum spanning tree and splitting it into several unrelated parts, we effectively remove the noise in the flow data. Furthermore, a recursive two-way optimum cut-based method is utilized to partition the graph composed of OD flows into OD flow clusters. Moreover, a criterion called CSSC (Child tree/Child graph Self-Similarity Criterion) is formulated to determine if the clusters meet the output requirements. By modifying the parameters, TOCOFC can obtain clustering results for different time scales and spatial scales, which makes it possible to study movement patterns from a multiscale perspective. However, TOCOFC has the disadvantages of low efficiency and large memory consumption, and it is not conducive to quickly handling large-scale data. Compared with previous works, TOCOFC has a better clustering performance, which is reflected in the fact that TOCOFC can guarantee a balance between clusters and help to fully understand the corresponding patterns. Being able to perform the spatiotemporal clustering of OD flows is also a highlight of TOCOFC, which will help to capture the differences in the patterns at different times for a deeper analysis. Extensive experiments on both artificial spatial datasets and real-world spatiotemporal datasets have demonstrated the effectiveness and flexibility of TOCOFC.
An Efficient and Scene-Adaptive Algorithm for Vehicle Detection in Aerial Images Using an Improved YOLOv3 Framework
Authors: Zhang Xunxun , Zhu Xu
Published: October 2019

Vehicle detection in aerial images has attracted great attention as an approach to providing the necessary information for transportation road network planning and traffic management. However, because of the low resolution, complex scene, occlusion, shadows, and high requirement for detection efficiency, implementing vehicle detection in aerial images is challenging. Therefore, we propose an efficient and scene-adaptive algorithm for vehicle detection in aerial images using an improved YOLOv3 framework, and it is applied to not only aerial still images but also videos composed of consecutive frame images. First, rather than directly using the traditional YOLOv3 network, we construct a new structure with fewer layers to improve the detection efficiency. Then, since complex scenes in aerial images can cause the partial occlusion of vehicles, we construct a context-aware-based feature map fusion to make full use of the information in the adjacent frames and accurately detect partially occluded vehicles. The traditional YOLOv3 network adopts a horizontal bounding box, which can attain the expected detection effects only for vehicles with small length–width ratio. Moreover, vehicles that are close to each other are liable to cause lower accuracy and a higher detection error rate. Hence, we design a sloping bounding box attached to the angle of the target vehicles. This modification is conducive to predicting not only the position but also the angle. Finally, two data sets were used to perform extensive experiments and comparisons. The results show that the proposed algorithm generates the desired and excellent performance.
A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
Authors: Pedrosa Ana G. , Francisco Tânia , Ferreira Maria J. , Rodrigues Tony A. , Barros-Barbosa Aurora , Azevedo Jorge E.
Published: October 2019

In contrast to many protein translocases that use ATP or GTP hydrolysis as the driving force to transport proteins across biological membranes, the peroxisomal matrix protein import machinery relies on a regulated self-assembly mechanism for this purpose and uses ATP hydrolysis only to reset its components. The ATP-dependent protein complex in charge of resetting this machinery—the Receptor Export Module (REM)—comprises two members of the “ATPases Associated with diverse cellular Activities” (AAA+) family, PEX1 and PEX6, and a membrane protein that anchors the ATPases to the organelle membrane. In recent years, a large amount of data on the structure/function of the REM complex has become available. Here, we discuss the main findings and their mechanistic implications.
Novel Crosstalks between Circadian Clock and Jasmonic Acid Pathway Finely Coordinate the Tradeoff among Plant Growth, Senescence and Defense
Authors: Zhang Yuanyuan , Bo Cunpei , Wang Lei
Published: October 2019

Circadian clock not only functions as a cellular time-keeping mechanism, but also acts as a master regulator to coordinate the tradeoff between plant growth and defense in higher plants by timing a few kinds of phytohormone biosynthesis and signaling, including jasmonic acid (JA). Notably, circadian clock and JA pathway have recently been shown to intertwine with each other to ensure and optimize the plant fitness in an ever-changing environment. It has clearly demonstrated that there are multiple crosstalk pathways between circadian clock and JA at both transcriptional and post-transcriptional levels. In this scenario, circadian clock temporally modulates JA-mediated plant development events, herbivory resistance and susceptibility to pathogen. By contrast, the JA signaling regulates clock activity in a feedback manner. In this review, we summarized the cross networks between circadian clock and JA pathway at both transcriptional and post-transcriptional levels. We proposed that the novel crosstalks between circadian clock and JA pathway not only benefit for the understanding the JA-associated circadian outputs including leaf senescence, biotic, and abiotic defenses, but also put timing as a new key factor to investigate JA pathway in the future.
Polyunsaturated Fatty Acids: What is Their Role in Treatment of Psychiatric Disorders?
Authors: Bozzatello Paola , Rocca Paola , Mantelli Emanuela , Bellino Silvio
Published: October 2019

In the central nervous system omega-3 fatty acids modulate cell signaling and affect dopaminergic and serotonergic pathways. On this basis, a new application for omega-3 fatty acids has been proposed, concerning the treatment of several psychiatric disorders. The present article is an update of a previous systematic review and is aimed to provide a complete report of data published in the period between 1980 and 2019 on efficacy and tolerability of omega-3 fatty acids in psychiatric disorders. In July 2019, an electronic search on PUBMED, Medline and PsychINFO of all RCTs, systematic reviews and meta-analyses on omega-3 fatty acids and psychiatric disorders without any filter or MESH restriction was performed. After eligibility processes, the final number of records included in this review was 126. One hundred and two of these studies were RCTs, while 24 were reviews and meta-analyses. The role of omega-3 fatty acids was studied in schizophrenia, major depression, bipolar disorder, anxiety disorders, obsessive-compulsive disorder, post-traumatic stress disorder, attention deficit hyperactivity disorder (ADHD), autism spectrum disorders, eating disorders, substance use disorder and borderline personality disorder. The main evidence of the efficacy of omega-3 fatty acids has been obtained in treating depressive symptoms in patients with major depression and, to a lesser degree, bipolar depression. Some efficacy was also found in early phases of schizophrenia in addition to antipsychotic treatment, but not in the chronic phases of psychosis. Small beneficial effects of omega-3 fatty acids were observed in ADHD and positive results were reported in a few trials on core symptoms of borderline personality disorder. For other psychiatric disorders results are inconsistent.
Cell Wall Proteins Play Critical Roles in Plant Adaptation to Phosphorus Deficiency
Authors: Wu Weiwei , Zhu Shengnan , Chen Qianqian , Lin Yan , Tian Jiang , Liang Cuiyue
Published: October 2019

Phosphorus is one of the mineral nutrient elements essential for plant growth and development. Low phosphate (Pi) availability in soils adversely affects crop production. To cope with low P stress, remodeling of root morphology and architecture is generally observed in plants, which must be accompanied by root cell wall modifications. It has been documented that cell wall proteins (CWPs) play critical roles in shaping cell walls, transmitting signals, and protecting cells against environmental stresses. However, understanding of the functions of CWPs involved in plant adaptation to P deficiency remains fragmentary. The aim of this review was to summarize advances in identification and functional characterization of CWPs in responses to P deficiency, and to highlight the critical roles of CWPs in mediating root growth, P reutilization, and mobilization in plants.
Comprehensive Assessment of GPR68 Expression in Normal and Neoplastic Human Tissues Using a Novel Rabbit Monoclonal Antibody
Authors: Herzig Markus , Dasgupta Pooja , Kaemmerer Daniel , Sänger Jörg , Evert Katja , Schulz Stefan , Lupp Amelie
Published: October 2019

GPR68 (OGR1) belongs to the proton-sensing G protein-coupled receptors that are involved in cellular adaptations to pH changes during tumour development. Although expression of GPR68 has been described in many tumour cell lines, little is known about its presence in human tumour entities. We characterised the novel rabbit monoclonal anti-human GPR68 antibody 16H23L16 using various cell lines and tissue specimens. The antibody was then applied to a large series of formalin-fixed, paraffin-embedded normal and neoplastic human tissue samples. Antibody specificity was demonstrated in a Western blot analysis of GPR68-expressing cells using specific siRNAs. Immunocytochemical experiments revealed pH-dependent changes in subcellular localisation of the receptor and internalisation after stimulation with lorazepam. In normal tissue, GPR68 was present in glucagon-producing islet cells, neuroendocrine cells of the intestinal tract, gastric glands, granulocytes, macrophages, muscle layers of arteries and arterioles, and capillaries. GPR68 was also expressed in neuroendocrine tumours, where it may be a positive prognostic factor, in pheochromocytomas, cervical adenocarcinomas, and endometrial cancer, as well as in paragangliomas, medullary thyroid carcinomas, gastrointestinal stromal tumours, and pancreatic adenocarcinomas. Often, tumour capillaries were also strongly GPR68-positive. The novel antibody 16H23L16 will be a valuable tool for basic research and for identifying GPR68-expressing tumours during histopathological examinations.
The Role of Growth Hormone in Mesenchymal Stem Cell Commitment
Authors: Bolamperti Simona , Guidobono Francesca , Rubinacci Alessandro , Villa Isabella
Published: October 2019

Growth hormone (GH) is best known for its prominent role in promoting prepubertal growth and in regulating body composition and metabolism during adulthood. In recent years, the possible role of GH in the modulation of mesenchymal stem cell (MSC) commitment has gained interest. MSCs, characterized by active self-renewal and differentiation potential, express GH receptors. In MSCs derived from different adult tissues, GH induces an inhibition of adipogenic differentiation and favors MSC differentiation towards osteogenesis. This activity of GH indicates that regulation of body composition by GH has already started in the tissue progenitor cells. These findings have fostered research on possible uses of MSCs treated with GH in those pathologies, where a lack of or delays in bone repair occur. After an overview of GH activities, this review will focus on the research that has characterized GH’s effects on MSCs and on preliminary studies on the possible application of GH in bone regenerative medicine.
Ion Transport Study in CS: POZ Based Polymer Membrane Electrolytes Using Trukhan Model
Authors: Aziz Shujahadeen B. , Karim Wrya O. , Brza M. A. , Abdulwahid Rebar T. , Saeed Salah Raza , Al-Zangana Shakhawan , Kadir M. F. Z.
Published: October 2019

In this work, analysis of ion transport parameters of polymer blend electrolytes incorporated with magnesium trifluoromethanesulfonate (Mg(CF3SO3)2) was carried out by employing the Trukhan model. A solution cast technique was used to obtain the polymer blend electrolytes composed of chitosan (CS) and poly (2-ethyl-2-oxazoline) (POZ). From X-ray diffraction (XRD) patterns, improvement in amorphous phase for the blend samples has been observed in comparison to the pure state of CS. From impedance plot, bulk resistance (Rb) was found to decrease with increasing temperature. Based on direct current (DC) conductivity (σdc) patterns, considerations on the ion transport models of Arrhenius and Vogel–Tammann–Fulcher (VTF) were given. Analysis of the dielectric properties was carried out at different temperatures and the obtained results were linked to the ion transport mechanism. It is demonstrated in the real part of electrical modulus that chitosan-salt systems are extremely capacitive. The asymmetric peak of the imaginary part (Mi) of electric modulus indicated that there is non-Debye type of relaxation for ions. From frequency dependence of dielectric loss (ε″) and the imaginary part (Mi) of electric modulus, suitable coupling among polymer segmental and ionic motions was identified. Two techniques were used to analyze the viscoelastic relaxation dynamic of ions. The Trukhan model was used to determine the diffusion coefficient (D) by using the frequency related to peak frequencies and loss tangent maximum heights (tanδmax). The Einstein–Nernst equation was applied to determine the carrier number density (n) and mobility. The ion transport parameters, such as D, n and mobility (μ), at room temperature, were found to be 4 × 10−5 cm2/s, 3.4 × 1015 cm−3, and 1.2 × 10−4 cm2/Vs, respectively. Finally, it was shown that an increase in temperature can also cause these parameters to increase.
Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes
Authors: Lewis Matthew T. , Kasper Jonathan D. , Bazil Jason N. , Frisbee Jefferson C. , Wiseman Robert W.
Published: October 2019

Type 2 diabetes (T2D) is a growing health concern with nearly 400 million affected worldwide as of 2014. T2D presents with hyperglycemia and insulin resistance resulting in increased risk for blindness, renal failure, nerve damage, and premature death. Skeletal muscle is a major site for insulin resistance and is responsible for up to 80% of glucose uptake during euglycemic hyperglycemic clamps. Glucose uptake in skeletal muscle is driven by mitochondrial oxidative phosphorylation and for this reason mitochondrial dysfunction has been implicated in T2D. In this review we integrate mitochondrial function with physiologic function to present a broader understanding of mitochondrial functional status in T2D utilizing studies from both human and rodent models. Quantification of mitochondrial function is explained both in vitro and in vivo highlighting the use of proper controls and the complications imposed by obesity and sedentary lifestyle. This review suggests that skeletal muscle mitochondria are not necessarily dysfunctional but limited oxygen supply to working muscle creates this misperception. Finally, we propose changes in experimental design to address this question unequivocally. If mitochondrial function is not impaired it suggests that therapeutic interventions and drug development must move away from the organelle and toward the cardiovascular system.
Vitamin D Receptor Gene Expression in Adipose Tissue of Obese Individuals is Regulated by miRNA and Correlates with the Pro-Inflammatory Cytokine Level
Authors: Jonas Marta Izabela , Kuryłowicz Alina , Bartoszewicz Zbigniew , Lisik Wojciech , Jonas Maurycy , Kozniewski Krzysztof , Puzianowska-Kuznicka Monika
Published: October 2019

Background: Given the role that vitamin D (VD) plays in the regulation of the inflammatory activity of adipocytes, we aimed to assess whether obesity changes the expression of VD-related genes in adipose tissue and, if so, to investigate whether this phenomenon depends on microRNA interference and how it may influence the local inflammatory milieu. Methods: The expression of genes encoding VD 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1) and receptor (VDR), selected interleukins and microRNAs was evaluated by real-time PCR in visceral (VAT) and in subcutaneous (SAT) adipose tissues of 55 obese (BMI > 40 kg/m2) and 31 normal-weight (BMI 20–24.9 kg/m2) individuals. Results: VDR mRNA levels were higher, while CYP27B1 levels were lower in adipose tissues of obese patients than in those of normal-weight controls (VAT: P = 0.04, SAT: P < 0.0001 and VAT: P = 0.004, SAT: P = 0.016, respectively). The expression of VDR in VAT of obese subjects correlated negatively with levels of miR-125a-5p (P = 0.0006, rs = −0.525), miR-125b-5p (P = 0.001, rs = −0.495), and miR-214-3p (P = 0.009, rs = −0.379). Additionally, VDR mRNA concentrations in visceral adipose tissues of obese subjects correlated positively with mRNA levels of interleukins: 1β, 6 and 8. Conclusions: We observed obesity-associated up-regulation of VDR and down-regulation of CYP27B mRNA levels in adipose tissue. VDR expression correlates with the expression of pro-inflammatory cytokines and may be regulated by miRNAs.
The CCR4–NOT Deadenylase Complex Maintains Adipocyte Identity
Authors: Takahashi Akinori , Takaoka Shohei , Kobori Shungo , Yamaguchi Tomokazu , Ferwati Sara , Kuba Keiji , Yamamoto Tadashi , Suzuki Toru
Published: October 2019

Shortening of poly(A) tails triggers mRNA degradation; hence, mRNA deadenylation regulates many biological events. In the present study, we generated mice lacking the Cnot1 gene, which encodes an essential scaffold subunit of the CCR4–NOT deadenylase complex in adipose tissues (Cnot1-AKO mice) and we examined the role of CCR4–NOT in adipocyte function. Cnot1-AKO mice showed reduced masses of white adipose tissue (WAT) and brown adipose tissue (BAT), indicating abnormal organization and function of those tissues. Indeed, Cnot1-AKO mice showed hyperinsulinemia, hyperglycemia, insulin resistance, and glucose intolerance and they could not maintain a normal body temperature during cold exposure. Muscle-like fibrous material appeared in both WAT and BAT of Cnot1-AKO mice, suggesting the acquisition of non-adipose tissue characteristics. Gene expression analysis using RNA-sequencing (RNA-seq) showed that the levels of adipose tissue-related mRNAs, including those of metabolic genes, decreased, whereas the levels of inflammatory response-related mRNAs increased. These data suggest that the CCR4–NOT complex ensures proper adipose tissue function by maintaining adipocyte-specific mRNAs at appropriate levels and by simultaneously suppressing mRNAs that would impair adipocyte function if overexpressed.
Molecular Cloning and Exploration of the Biochemical and Functional Analysis of Recombinant Glucose-6-Phosphate Dehydrogenase from Gluconoacetobacter diazotrophicus PAL5
Authors: Ramírez-Nava Edson , Ortega-Cuellar Daniel , González-Valdez Abigail , Castillo-Rodríguez Rosa , Ponce-Soto Gabriel , Hernández-Ochoa Beatriz , Cárdenas-Rodríguez Noemí , Martínez-Rosas Víctor , Morales-Luna Laura , Serrano-Posada Hugo , Sierra-Palacios Edgar , Arreguin-Espinosa Roberto , Cuevas-Cruz Miguel , Rocha-Ramírez Luz , Pérez de la Cruz Verónica , Marcial-Quino Jaime , Gómez-Manzo Saúl
Published: October 2019

Gluconacetobacter diazotrophicus PAL5 (GDI) is an endophytic bacterium with potential biotechnological applications in industry and agronomy. The recent description of its complete genome and its principal metabolic enzymes suggests that glucose metabolism is accomplished through the pentose phosphate pathway (PPP); however, the enzymes participating in this pathway have not yet been characterized in detail. The objective of the present work was to clone, purify, and biochemically and physicochemically characterize glucose-6-phosphate dehydrogenase (G6PD) from GDI. The gene was cloned and expressed as a tagged protein in E. coli to be purified by affinity chromatography. The native state of the G6PD protein in the solution was found to be a tetramer with optimal activity at pH 8.8 and a temperature between 37 and 50 °C. The apparent Km values for G6P and nicotinamide adenine dinucleotide phosphate (NADP+) were 63 and 7.2 μM, respectively. Finally, from the amino acid sequence a three-dimensional (3D) model was obtained, which allowed the arrangement of the amino acids involved in the catalytic activity, which are conserved (RIDHYLGKE, GxGGDLT, and EKPxG) with those of other species, to be identified. This characterization of the enzyme could help to identify new environmental conditions for the knowledge of the plant–microorganism interactions and a better use of GDI in new technological applications.
Long Non-Coding RNA GAS5 and Intestinal MMP2 and MMP9 Expression: A Translational Study in Pediatric Patients with IBD
Authors: Lucafò Marianna , Pugnetti Letizia , Bramuzzo Matteo , Curci Debora , Di Silvestre Alessia , Marcuzzi Annalisa , Bergamo Alberta , Martelossi Stefano , Villanacci Vincenzo , Bozzola Anna , Cadei Moris , De Iudicibus Sara , Decorti Giuliana , Stocco Gabriele
Published: October 2019

Background: The long non-coding RNA (lncRNA) growth arrest–specific transcript 5 (GAS5) seems to be involved in the regulation of mediators of tissue injury, in particular matrix metalloproteinases (MMPs), implicated in the pathogenesis of inflammatory bowel disease (IBD). We investigated the role of GAS5 in regulating MMP2 and MMP9 expression in pediatric patients with IBD and in vitro. Methods: In total, 25 IBD patients were enrolled: For each patient paired inflamed and non-inflamed biopsies were collected. RNA was extracted and GAS5, MMP2, and MMP9 were quantified by TaqMan assay. The expression of GAS5 and MMPs was also determined in the human monocytic THP1 cells differentiated into macrophages and stimulated with lipopolysaccharide (LPS). The function of GAS5 was assessed by overexpressing the lncRNA and evaluating the MMPs levels. Results: Real-time PCR results demonstrated a downregulation of GAS5 and an upregulation of both MMPs in inflamed tissues. In vitro data confirmed the trend observed in patients for the three genes: The stimulation with LPS promoted a downregulation of GAS5 while an increase of MMPs was observed. Overexpression experiments showed that higher levels of GAS5 lead to a decrease of both enzymes. Conclusion: These results provide new information about the role of GAS5 in IBD: The lncRNA could mediate tissue damage by modulating the expression of MMPs.
Endophytic Bacteria Potentially Promote Plant Growth by Synthesizing Different Metabolites and their Phenotypic/Physiological Profiles in the Biolog GEN III MicroPlateTM Test
Authors: Woźniak Małgorzata , Gałązka Anna , Tyśkiewicz Renata , Jaroszuk-Ściseł Jolanta
Published: October 2019

Endophytic bacteria, as the most promising components of effective, biofertilizers biostimulating and biocontrol preparations, should be very intensively obtained from various plants and studied in terms of the conditions determining the potential ability to promote plant growth. For this reason, endophytic bacteria have been isolated from both stems and roots of up to six systematically distant species of vascular plants: one species belonging to the seedless vascular plants (Monilophyta), and five seed plants (Spermatophyta). The 23 isolated strains represented nine genera: Delftia, Stenotrophomonas, Rhizobium, Brevundimonas, Variovorax, Achromobacter, Novosphingobium, Comamonas and Collimonas, notably which were closely related—belonging to the phylum Proteobacteria. Stenotrophomonas sp. strains showed the greatest ability to synthesize indole-3-acetic acid (IAA)-like compounds, while Achromobacter sp. strains produced the highest levels of siderophores. The presence of the nifH gene and nitrogen binding activity was demonstrated for 95% of the strains tested. Stenotrophomonas maltophila (ES2 strain) showed the highest metabolic activity based on Biolog GEN III test. The ability to solubilize phosphate was determined only for three tested strains from genus: Delftia, Rhizobium and Novosphingobium. The presented work demonstrated that the metabolic and phenotypic properties of plant growth-promoting endophytes are correlated with the genus of bacteria and are not correlated with the host plant species or part of plant (stem, root).
Host Genotype and Precipitation Influence of Fungal Endophyte Symbiosis and Mycotoxin Abundance in a Locoweed
Authors: He Wei , Guo Linwei , Wang Lei , Zhao Qianqian , Guo Lizhu , Cao Wei , Mur Luis A. J. , Wei Yahui
Published: October 2019

Many plant endophytes produce mycotoxins, but how host genetic variation influences endophyte colonization and mycotoxin production under natural conditions is poorly understood. This interaction has not been fully considered in many previous studies which used controlled experiments with agronomic or model plant species. Here, we investigated this interaction in a naturally occurring forb (a locoweed species) Oxytropis ochrocephala, its symbiotic endophyte Alternaria oxytropis, and the mycotoxin swainsonine. Host genetic variation was characterized by microsatellite markers. Endophyte infection rate and swainsonine levels were determined by PCR and HPLC, respectively. Genetic markers defined two distinct host populations and revealed that host genetics were significantly correlated with geographical location, elevation, and precipitation. As the host diverged, symbiotic interactions were reduced or failed to produce detectable swainsonine in one host population. Host genotype and precipitation had a significant impact in shaping swainsonine production at the population level. This study highlights the effect of host genotype in influencing this interaction in locoweeds.
The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3)
Authors: Caccuri Francesca , Bugatti Antonella , Corbellini Silvia , Roversi Sara , Zani Alberto , Mazzuca Pietro , Marsico Stefania , Caruso Arnaldo , Giagulli Cinzia
Published: October 2019

In recent years immunomodulators have gained a strong interest and represent nowadays an active expanding area of research for the control of microbial diseases and for their therapeutic potential in preventing, treating and reducing the morbidity and mortality of different diseases. Pidotimod (3-L-pyroglutamyl-L-thiaziolidine-4carboxylic acid, PDT) is a synthetic dipeptide, which possesses immunomodulatory properties and exerts a well-defined pharmacological activity against infections, but its real mechanism of action is still undefined. Here, we show that PDT is capable of activating tyrosine phosphorylation-based cell signaling in human primary monocytes and triggering rapid adhesion and chemotaxis. PDT-induced monocyte migration requires the activation of the PI3K/Akt signaling pathway and chemokine receptor CXCR3. Indeed, a mAb to CXCR3 and a specific receptor inhibitor suppressed significantly PDT-dependent chemotaxis, and CXCR3-silenced primary monocytes lost responsiveness to PDT chemoattraction. Moreover, our results highlighted that the PDT-induced migratory activity is sustained by the CXCR3A isoform, since CXCR3-transfected L1.2 cells acquired responsiveness to PDT stimulation. Finally, we show that PDT, as CXCR3 ligands, is also able to direct the migration of IL-2 activated T cells, which express the highest levels of CXCR3 among CXCR3-expressing cells. In conclusion, our study defines a chemokine-like activity for PDT through CXCR3A and points on the possible role that this synthetic dipeptide may play in leukocyte trafficking and function. Since recent studies have highlighted diverse therapeutic roles for molecules which activates CXCR3, our findings call for an exploration of using this dipeptide in different pathological processes.