bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of Open-Ended Structure Wideband PCB Rogowski Coil Based on New Winding Method
Authors: Tan Qinghua , Zhang Wenbin , Tan Xiangyu , Yang Le , Ren Yanan , Hu Yang
Published: January 2022

Compared with the traditional Rogowski coil, printed circuit board (PCB) Rogowski coil has the advantages of high measurement accuracy, simple manufacturing process and low cost. It has become an important device in current measurement. However, the existing PCB Rogowski coils are mostly closed, and the open-ended structure PCB Rogowski coils are susceptible to external magnetic field interference. This paper proposes an open-ended structure PCB Rogowski coil based on a new winding method, so that the two open-ended PCB Rogowski coils are evenly distributed when closed, while improving the coil’s immunity to external magnetic fields based on the winding method of equal area of the de-wired and loop. In addition, this paper uses the hybrid integration circuit and the back-end matching method to achieve the broadband measurement performance of the sensor. Finally, the experimental results show that the mutual inductance difference between the closed and open-ended structure PCB Rogowski coil is less than 0.5% under the same wiring mode. Under the equivalent current interference at 50 mm from the measured current, the maximum interference coefficient of the open-ended structure coil is 1.45%; in sine sweep and pulse current test conditions, the frequency band is 34 Hz–3.5 MHz.
Techniques for SAT-Based Boolean Reasoning on Multiple Faults Affecting Logic Cells and Interconnects in Digital ICs
Authors: Dall’Occo Francesco , Dalpasso Marcello , Favalli Michele
Published: January 2022

We propose a new method for SAT-based Boolean reasoning on multiple defects in digital ICs. Although it does not explicitly consider a specific fault model such as model-based techniques, it allows us to consider more realistic cases than model-free approaches. In particular, it can be used to account for (a) faults resulting in monotonic errors at the output of a cell and (b) faults, such as breaks or bridgings, that may corrupt the propagation of a signal from its fan-out branches. The model can be used for either standard gates or more complex combinational modules. Examples are shown for applications requiring the consideration of multiple defects such as fault diagnosis and reliability analysis. The feasibility of the proposed approach is assessed by results on a set of combinational benchmarks.
3D Placement of a New Tethered UAV to UAV Relay System for Coverage Maximization
Authors: Safwat Nour El-Din , Hafez Ismail Mohammed , Newagy Fatma
Published: January 2022

In this paper, a new relay system that uses the UAV as a relay station between the tethered UAV and ground user (TU2U2G) is proposed. The TU2U2G system uses a TUAV as a viable alternative to replace BS and provide seamless service over a cable that simultaneously supplies stable power and a reliable wired data-link connection from a ground control station. Compared to the BS, TUAV improves system coverage due to its high altitude. Also, it overcomes the antenna down-tilting, which increases the path loss between BS and UAV in the cellular system. In addition, it overcomes the UAV drawback of the batteries’ limited capacity. Therefore, TUAV can achieve the main requirements of a reliable cellular BS in terms of endurance, backhaul link quality, and the advantage of the UAV’s high altitude. After that, the optimization problem is formulated to maximize UAV relay station coverage under the power budget and maximum UAV height constraints. For simplicity, the 3D placement of the UAV is decoupled to the vertical and horizontal placement. Then, a 3D placement algorithm for the system is proposed. The UAV placement in the TU2U2G system compared to the cellular system shows better results in terms of optimum UAV height, maximum coverage radius, and maximum relaying distance.
Enhancing Monitoring and Control of an HVAC System through IoT
Authors: Tanasiev Vladimir , Pluteanu Ștefăniță , Necula Horia , Pătrașcu Roxana
Published: January 2022

Throughout time, European policies on climate and energy change have generated several transformations in the building sector. In this endeavour, digital technologies have played an essential role in energy efficiency through process optimisation. However, data integration and exchange between different solutions is still challenging to achieve. With the advancement of Internet of Things (IoT) based solutions, one of the latest innovations, new opportunities are envisioned. IoT solutions have been implemented in smart grids, intelligent buildings, smart cities, and other sectors in recent years. Following the guidelines of ISA/ANSI 95-Enterprise-Control System Integration, the edge computing apps and cloud platform are needed to solve data bottlenecks, data ingestion, data integration aspects, bringing at the same time new knowledge related to user comfort, energy management and accessibility. This research focuses on a case study that shows how the thermal comfort of a building can be controlled and improved through an IoT vertically integrated solution. The deployed solution opens new possibilities for cross-functional integration and data aggregation, an essential step towards energy efficiency.
Evaluation of Bacteria and Fungi DNA Abundance in Human Tissues
Authors: de Albuquerque Gabriela E. , Moda Bruno S. , Serpa Marianna S. , Branco Gabriela P. , Defelicibus Alexandre , Takenaka Isabella K. T. M. , de Amorim Maria G. , Miola Elizabeth C. , Martins Valquiria C. A. , Torres Katia L. , Bezerra Stephania M. , Claro Laura C. L. , Pelosof Adriane G. , Sztokfisz Claudia Z. , Abrantes Lais L. S. , Coimbra Felipe J. F. , Kowalski Luiz P. , Alves Fábio A. , Zequi Stênio C. , Udekwu Klas I. , Silva Israel T. , Nunes Diana N. , Bartelli Thais F. , Dias-Neto Emmanuel
Published: January 2022

Whereas targeted and shotgun sequencing approaches are both powerful in allowing the study of tissue-associated microbiota, the human: microorganism abundance ratios in tissues of interest will ultimately determine the most suitable sequencing approach. In addition, it is possible that the knowledge of the relative abundance of bacteria and fungi during a treatment course or in pathological conditions can be relevant in many medical conditions. Here, we present a qPCR-targeted approach to determine the absolute and relative amounts of bacteria and fungi and demonstrate their relative DNA abundance in nine different human tissue types for a total of 87 samples. In these tissues, fungi genomes are more abundant in stool and skin samples but have much lower levels in other tissues. Bacteria genomes prevail in stool, skin, oral swabs, saliva, and gastric fluids. These findings were confirmed by shotgun sequencing for stool and gastric fluids. This approach may contribute to a more comprehensive view of the human microbiota in targeted studies for assessing the abundance levels of microorganisms during disease treatment/progression and to indicate the most informative methods for studying microbial composition (shotgun versus targeted sequencing) for various samples types.
First Glimpse on Spring Starflower Domestication
Authors: Sassone Agostina B. , Blattner Frank R. , Giussani Liliana M. , Hojsgaard Diego H.
Published: January 2022

The cultivation and domestication of plants are human-driven processes that change the biology and attributes of a plant. Ipheion uniflorum is a bulbous geophyte known as Spring Starflower whose cultivation dates back to the first half of the 19th century. At least seven cultivars have been developed from natural stands. However, comparative analyses of wild and cultivated materials are largely missing. In the present study, we provide a morphological evaluation and analyses of the cytological and genetic variability of I. uniflorum that reveal significant levels of differentiation and evidence of artificial selection in the Spring Starflower. Distinctive phenotypic characters in cultivated materials that are rarely found or lacking in wild plants and natural populations, such as pink or violet flowers, together with its reduced heterozygosity and starting genetic differentiation support the view of early mechanisms of domestication acting upon Spring Starflower plants. The probable geographic origin of the cultivated forms is discussed together with perspectives for plant breeding.
Spatiotemporal Modeling of the Electricity Production from Variable Renewable Energies in Germany
Authors: Lehneis Reinhold , Manske David , Schinkel Björn , Thrän Daniela
Published: January 2022

In recent years, electricity production from wind turbines and photovoltaic systems has grown significantly in Germany. To determine the multiple impacts of rising variable renewable energies on an increasingly decentralized power supply, spatially and temporally resolved data on the power generation are necessary or, at least, very helpful. Because of extensive data protection regulations in Germany, especially for smaller operators of renewable power plants, such detailed data are not freely accessible. In order to fill this information gap, simulation models employing publicly available plant and weather data can be used. The numerical simulations are performed for the year 2016 and consider an ensemble of almost 1.64 million variable renewable power plants in Germany. The obtained time series achieve a high agreement with measured feed-in patterns over the investigated year. Such disaggregated power generation data are very advantageous to analyze the energy transition in Germany on a spatiotemporally resolved scale. In addition, this study also derives meaningful key figures for such an analysis and presents the generated results as detailed maps at county level. To the best of our knowledge, such highly resolved electricity data of variable renewables for the entire German region have never been shown before.
Comparative Study on Matching Methods for the Distinction of Building Modifications and Replacements Based on Multi-Temporal Building Footprint Data
Authors: Schorcht Martin , Hecht Robert , Meinel Gotthard
Published: January 2022

We compare different matching methods for distinguishing building modifications from replacements based on multi-temporal building footprint geometries from 3D city models. Manually referenced footprints of building changes were used to determine which thresholds are suitable for distinction. In addition, since the underlying LoD1 (Level of Detail 1) data is highly accurate, randomly generated position deviations were added to allow for transferability to less well-matched data. In order to generate a defined position deviation, a novel method was developed. This allows determination of the effects of position deviations on accuracy. Determination of these methods’ suitability for manipulation of data from sources of different levels of generalization (cross-scale matching) is therefore not the focus of this work. In detail, the methods of ‘Common Area Ratio’, ‘Common Boundary Ratio’, ‘Hausdorff Distance’ and ‘PoLiS’ (Polygon and Line Segment based metric) were compared. In addition, we developed an extended line-based procedure, which we called ‘Intersection Boundary Ratio’. This method was shown to be more robust than the previous matching methods for small position deviations. Furthermore, we addressed the question of whether a minimum function at PoLiS and Hausdorff distance is more suitable to distinguish between modification and replacement.
Influence of Hypoxia on Radiosensitization of Cancer Cells by 5-Bromo-2′-deoxyuridine
Authors: Zdrowowicz Magdalena , Spisz Paulina , Hać Aleksandra , Herman-Antosiewicz Anna , Rak Janusz
Published: January 2022

Radiotherapy is a crucial cancer treatment, but its outcome is still far from satisfactory. One of the reasons that cancer cells show resistance to ionizing radiation is hypoxia, defined as a low level of oxygenation, which is typical for solid tumors. In the hypoxic environment, cancer cells are 2–3 times more resistant to ionizing radiation than normoxic cells. To overcome this important impediment, radiosensitizers should be introduced to cancer therapy. When modified with an electrophilic substituent, nucleosides may undergo efficient dissociative electron attachment (DEA) that leaves behind nucleoside radicals, which, in secondary reactions, are able to induce DNA damage, leading to cancer cell death. We report the radiosensitizing effect of one of the best-known DEA-type radiosensitizers—5-bromo-2′-deoxyuridine (BrdU)—on breast (MCF-7) and prostate (PC3) cancer cells under both normoxia and hypoxia. MCF-7 and PC3 cells were treated with BrdU to investigate the effect of hypoxia on cell proliferation, incorporation into DNA and radiosensitivity. While the oxygen concentration did not significantly affect the efficiency of BrdU incorporation into DNA or the proliferation of tumor cells, the radiosensitizing effect of BrdU on hypoxic cells was more evident than on normoxic cells. Further mechanistic studies performed with the use of flow cytometry showed that under hypoxia, BrdU increased the level of histone H2A.X phosphorylation after X-ray exposure to a greater extent than under normal oxygenation conditions. These results confirm that the formation of double-strand breaks in hypoxic BrdU-treated cancer cells is more efficient. In addition, by performing stationary radiolysis of BrdU solution in the presence of an ●OH radical scavenger, we compared the degree of its electron-induced degradation under aerobic and anaerobic conditions. It was determined that radiodegradation under anaerobic conditions was almost twice as high as that under aerobic conditions.
The Role of Vitamin D in Sleep Disorders of Children and Adolescents: A Systematic Review
Authors: Prono Federica , Bernardi Katerina , Ferri Raffaele , Bruni Oliviero
Published: January 2022

This review investigates the association between vitamin D and sleep disorders. Vitamin D is an essential nutrient known to play an important role in the growth and bone health of the human body, but it also appears to play a role in sleep. The goal of our review is to examine the association between vitamin D and sleep disorders in children and adolescents. We summarize the evidence about the role and the mechanism of action of vitamin D in children and adolescents with sleep disorders such as insomnia, obstructive sleep apnea (OSA), restless legs syndrome (RLS), and other sleep disorders. Systematic electronic database searches were conducted using Pubmed and Cochrane Library. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline was followed. The studies that met the established inclusion criteria were analyzed and compared. Results suggest a strict relationship between vitamin D deficiency in children and sleep disorders. There is evidence that vitamin D is implicated in the different neurochemical mechanisms involved in sleep regulation and mainly in the serotonergic and dopaminergic pathways. This might be responsible for the association of vitamin D deficiency and restless sleep, sleep hyperhidrosis, OSA, and RLS.
The Effect of C-Phycocyanin on Microglia Activation Is Mediated by Toll-like Receptor 4
Authors: Piovan Anna , Filippini Raffaella , Argentini Carla , Moro Stefano , Giusti Pietro , Zusso Morena
Published: January 2022

The blue-green alga Spirulina platensis is rich in phycocyanins, that exhibit a wide range of pharmacological actions. C-phycocyanin (C-PC), in particular, possesses hepatoprotective, nephroprotective, antioxidant, and anticancer effects. Furthermore, several studies have reported both anti- and proinflammatory properties of this pigment. However, the precise mechanism(s) of action of C-PC in these processes remain largely unknown. Therefore, here we explored the C-PC effect in in vitro microglia activation. The effect of C-PC on the expression and release of IL-1β and TNF-α and the activation of NF-κB was examined in primary microglia by real-time PCR, ELISA, and immunofluorescence. Treatment with C-PC up-regulated the expression and release of IL-1β and TNF-α. C-PC also promoted the nuclear translocation of the NF-κB transcription factor. Then, to elucidate the molecular mechanisms for the immunoregulatory function of C-PC, we focused on investigating the role of Toll-like receptor 4 (TLR4). Accordingly, several TLR4 inhibitors have been used. Curcumin, ciprofloxacin, L48H37, and CLI-095 that suppresses specifically TLR4 signaling, blocked IL-1β and TNF-α. Overall, these results indicate the immunomodulatory effect of C-PC in microglia cultures and show for the first time that the molecular mechanism implicated in this effect may involve TLR4 activation.
Bioactive Peptides: Synthesis, Sources, Applications, and Proposed Mechanisms of Action
Authors: Akbarian Mohsen , Khani Ali , Eghbalpour Sara , Uversky Vladimir N.
Published: January 2022

Bioactive peptides are a group of biological molecules that are normally buried in the structure of parent proteins and become active after the cleavage of the proteins. Another group of peptides is actively produced and found in many microorganisms and the body of organisms. Today, many groups of bioactive peptides have been marketed chemically or recombinantly. This article reviews the various production methods and sources of these important/ubiquitous and useful biomolecules. Their applications, such as antimicrobial, antihypertensive, antioxidant activities, blood-lipid-lowering effect, opioid role, antiobesity, ability to bind minerals, antidiabetic, and antiaging effects, will be explored. The types of pathways proposed for bioactive applications will be in the next part of the article, and at the end, the future perspectives of bioactive peptides will be reviewed. Reading this article is recommended for researchers interested in various fields of physiology, microbiology, biochemistry, and nanotechnology and food industry professionals.
Transcriptomic Profile of Canine DH82 Macrophages Infected by Leishmania infantum Promastigotes with Different Virulence Behavior
Authors: Mas Alicia , Martínez-Rodrigo Abel , Carrión Javier , Orden José Antonio , Alzate Juan F. , Domínguez-Bernal Gustavo , Horcajo Pilar
Published: January 2022

Zoonotic visceral leishmaniosis caused by Leishmania infantum is an endemic disease in the Mediterranean Basin affecting mainly humans and dogs, the main reservoir. The leishmaniosis outbreak declared in the Community of Madrid (Spain) led to a significant increase in human disease incidence without enhancing canine leishmaniosis prevalence, suggesting a better adaptation of the outbreak’s isolates by other host species. One of the isolates obtained in the focus, IPER/ES/2012/BOS1FL1 (BOS1FL1), has previously demonstrated a different phenotype than the reference strain MCAN/ES/1996/BCN150 (BCN150), characterized by a lower infectivity when interacting with canine macrophages. Nevertheless, not enough changes in the cell defensive response were found to support their different behavior. Thus, we decided to investigate the molecular mechanisms involved in the interaction of both parasites with DH82 canine macrophages by studying their transcriptomic profiles developed after infection using RNA sequencing. The results showed a common regulation induced by both parasites in the phosphoinositide-3-kinase–protein kinase B/Akt and NOD-like receptor signaling pathways. However, other pathways, such as phagocytosis and signal transduction, including tumor necrosis factor, mitogen-activated kinases and nuclear factor-κB, were only regulated after infection with BOS1FL1. These differences could contribute to the reduced infection ability of the outbreak isolates in canine cells. Our results open a new avenue to investigate the true role of adaptation of L. infantum isolates in their interaction with their different hosts.
Role of Autophagy in Lysophosphatidylcholine-Induced Apoptosis of Mouse Ovarian Granulosa Cells
Authors: Yang Si , Chen Jie , Ma Bingchun , Wang Jinglei , Chen Jiaxiang
Published: January 2022

Lysophosphatidylcholine (LPC), also known as lysolecithin, is one of the major components of oxidized low-density lipoproteins (ox-LDL). In the pathogenetic process of diverse diseases, LPC acts as a significant lipid mediator. However, no evidence shows that LPC can affect the female reproductive system. In our study, we found that LPC inhibited the cell viability of primary mouse ovarian granulosa cells. Meanwhile, LPC was shown to induce apoptosis, which is accompanied by an increase in apoptosis-related protein levels, such as cleaved caspase-3, cleaved caspase-8 and Bax, as well as a decrease in Bcl-2. The total numbers of early and late apoptotic cells also increased in the LPC-treated cells. These results indicated that LPC could induce apoptosis of mouse ovarian granulosa cells. Furthermore, the increase in autophagy-related protein levels and the number of autophagic vesicles suggested that LPC could induce autophagy. The inhibition of oxidative stress by N-acetyl-L-cysteine (NAC) could rescue the induction of apoptosis and autophagy by LPC, which indicated that oxidative stress was involved in LPC-induced apoptosis and autophagy. Interestingly, the inhibition of autophagy by 3-MA could reserve the inhibition of cell viability and the induction of apoptosis by LPC. In conclusion, oxidative stress was involved in LPC-induced apoptosis, whileautophagy of mouse ovarian granulosa cells and the inhibition of autophagy could alleviate LPC-induced apoptosis.
Easily Established and Multifunctional Synthetic Nanobody Libraries as Research Tools
Authors: Liu Bingying , Yang Daiwen
Published: January 2022

Nanobodies, or VHHs, refer to the antigen-binding domain of heavy-chain antibodies (HCAbs) from camelids. They have been widely used as research tools for protein purification and structure determination due to their small size, high specificity, and high stability, overcoming limitations with conventional antibody fragments. However, animal immunization and subsequent retrieval of antigen-specific nanobodies are expensive and complicated. Construction of synthetic nanobody libraries using DNA oligonucleotides is a cost-effective alternative for immunization libraries and shows great potential in identifying antigen-specific or even conformation-specific nanobodies. This review summarizes and analyses synthetic nanobody libraries in the current literature, including library design and biopanning methods, and further discusses applications of antigen-specific nanobodies obtained from synthetic libraries to research.
Knockout of the ABCB1 Gene Increases Susceptibility to Emamectin Benzoate, Beta-Cypermethrin and Chlorantraniliprole in Spodoptera frugiperda
Authors: Li Qi , Jin Minghui , Yu Songmiao , Cheng Ying , Shan Yinxue , Wang Peng , Yuan Haibin , Xiao Yutao
Published: January 2022

ATP-binding cassette transporter B1 (ABCB1, or P-glycoprotein) is known to be an important participant in multidrug resistance in mammals, and it also has been proved as a transporter for some insecticides in several lepidopteran insects, yet the precise function of this transporter in Spodoptera frugiperda is unknown. Here, we generated a SfABCB1 knockout strain of the S. frugiperda using the CRISPR/Cas9 system to explore its potential roles in determining susceptibility to chemical insecticides or Bt toxins. Bioassay results showed that the susceptibility of SfABCB1 knockout strain to beta-cypermethrin, chlorantraniliprole and emamectin benzoate were significantly increased compared with the wild-type strain DH19, whereas there were no changes to Bt toxins for Cry1Ab, Cry1Fa and Vip3Aa. Our results revealed that SfABCB1 plays important roles in the susceptibility of S. frugiperda to beta-cypermethrin, chlorantraniliprole and emamectin benzoate, and imply that overexpression of ABCB1 may contribute to beta-cypermethrin, chlorantraniliprole and emamectin benzoate resistance in S. frugiperda.
Randomized Comparison of Two New Methods for Chest Compressions during CPR in Microgravity—A Manikin Study
Authors: Schmitz Jan , Ahlbäck Anton , DuCanto James , Kerkhoff Steffen , Komorowski Matthieu , Löw Vanessa , Russomano Thais , Starck Clement , Thierry Seamus , Warnecke Tobias , Hinkelbein Jochen
Published: January 2022

Background: Although there have been no reported cardiac arrests in space to date, the risk of severe medical events occurring during long-duration spaceflights is a major concern. These critical events can endanger both the crew as well as the mission and include cardiac arrest, which would require cardiopulmonary resuscitation (CPR). Thus far, five methods to perform CPR in microgravity have been proposed. However, each method seems insufficient to some extent and not applicable at all locations in a spacecraft. The aim of the present study is to describe and gather data for two new CPR methods in microgravity. Materials and Methods: A randomized, controlled trial (RCT) compared two new methods for CPR in a free-floating underwater setting. Paramedics performed chest compressions on a manikin (Ambu Man, Ambu, Germany) using two new methods for a free-floating position in a parallel-group design. The first method (Schmitz–Hinkelbein method) is similar to conventional CPR on earth, with the patient in a supine position lying on the operator’s knees for stabilization. The second method (Cologne method) is similar to the first, but chest compressions are conducted with one elbow while the other hand stabilizes the head. The main outcome parameters included the total number of chest compressions (n) during 1 min of CPR (compression rate), the rate of correct chest compressions (%), and no-flow time (s). The study was registered on clinicaltrials.gov (NCT04354883). Results: Fifteen volunteers (age 31.0 ± 8.8 years, height 180.3 ± 7.5 cm, and weight 84.1 ± 13.2 kg) participated in this study. Compared to the Cologne method, the Schmitz–Hinkelbein method showed superiority in compression rates (100.5 ± 14.4 compressions/min), correct compression depth (65 ± 23%), and overall high rates of correct thoracic release after compression (66% high, 20% moderate, and 13% low). The Cologne method showed correct depth rates (28 ± 27%) but was associated with a lower mean compression rate (73.9 ± 25.5/min) and with lower rates of correct thoracic release (20% high, 7% moderate, and 73% low). Conclusions: Both methods are feasible without any equipment and could enable immediate CPR during cardiac arrest in microgravity, even in a single-helper scenario. The Schmitz–Hinkelbein method appears superior and could allow the delivery of high-quality CPR immediately after cardiac arrest with sufficient quality.
Usefulness of Aerobic Exercise for Home Blood Pressure Control in Patients with Diabetes: Randomized Crossover Trial
Authors: Iwai Keiko , Ushigome Emi , Okada Kazufumi , Yokota Isao , Majima Saori , Nakanishi Naoko , Hashimoto Yoshitaka , Okada Hiroshi , Senmaru Takafumi , Hamaguchi Masahide , Asano Mai , Yamazaki Masahiro , Fukui Michiaki
Published: January 2022

Hypertension usually coexists with diabetes mellitus and significantly increases the risk of macrovascular complications. Blood pressure measured at home, especially nocturnal blood pressure, is particularly important because it is more strongly associated with target organ damage than clinical blood pressure measurements. Regular moderate aerobic exercise has been shown to have anti-hypertensive effects. This study aimed to investigate the effects of aerobic exercise on home blood pressure in patients with diabetes. This randomized crossover trial was based on outpatient treatment at a university hospital. In this randomized crossover trial, 124 patients with type 2 diabetes were randomly assigned to two groups over 56 days: an exercise preceding group (exercise intervention for 28 days and then no exercise intervention for the following 28 days) and an exercise lagging group (no exercise intervention for 28 days and then exercise intervention for the following 28 days). The associations between the nocturnal blood pressure and exercise intervention were assessed accordingly. A decrease in blood pressure was observed in the morning and evening, at 2 a.m. and 3 a.m. after exercise intervention; however, there was no significant difference between groups. Moderate exercise was not effective in lowering nocturnal blood pressure in this study.
Relationship between Multimorbidity and Quality of Life in a Primary Care Setting: The Mediating Role of Dyspnea
Authors: Alfano Pietro , Cuttitta Giuseppina , Audino Palma , Fazio Giovanni , La Grutta Sabina , Marcantonio Salvatore ,   Snamid Palermo Cooperative Group, Bucchieri Salvatore
Published: January 2022

Multimorbidity is known to impair Quality of Life (QoL) in patients in a primary setting. Poor QoL is associated with higher dyspnea perception. How multimorbidity and dyspnea perception are related to QoL needs clarification. The aim of the present study is to evaluate the mediating role of dyspnea perception in the relationship between multimorbidity and QoL in adults with and without airflow obstruction in a primary care setting. Seventeen general practitioners participated in the study: a total of 912 adult patients attending the practitioner’s surgery for a generic consultation completed a preliminary respiratory screening; 566 of them answered a respiratory questionnaire between January and June 2014, and 259 of the latter (148 M, aged 40–88) agreed to go through all the of procedures including spirometry, the IMCA and QoL (SF-36 through Physical Health “PCS” and Mental Health components) questionnaires, evaluation of comorbidities and the mMRC Dyspnea Scale. For screening purpose, a cut-off of FEV1/FVC < 70% was considered a marker of airflow obstruction (AO). Of the sample, 25% showed airflow obstruction (AO). No significant difference in mMRC score regarding the number of comorbidities and the PCS was found between subjects with and without AO. Multimorbidity and PCS were inversely related in subjects with (p < 0.001) and without AO (p < 0.001); mMRC and PCS were inversely related in subjects with (p = 0.001) and without AO (p < 0.001). A mediation analysis showed that the relation between number of comorbidities and PCS was totally mediated by mMRC in subjects with AO and partially in subjects without AO. We conclude that the effect of multimorbidity on PCS is totally mediated by mMRC only in AO. Detecting and monitoring mMRC in a primary care setting may be a useful indicator for evaluating a patient’s global health.
Mobile Single-Lead Electrocardiogram Technology for Atrial Fibrillation Detection in Acute Ischemic Stroke Patients
Authors: Leńska-Mieciek Marta , Kuls-Oszmaniec Aleksandra , Dociak Natalia , Kowalewski Marcin , Sarwiński Krzysztof , Osiecki Andrzej , Fiszer Urszula
Published: January 2022

(1) Background: AliveCor KardiaMobile (KM) is a portable electrocardiography recorder for detection of atrial fibrillation (AF). The aim of the study was to define the group of acute ischemic stroke (AIS) patients who can use the KM device and assess the diagnostic test accuracy. (2) Methods: the AIS patients were recruited to the study. Thirty-second single-lead electrocardiogram (ECG) usages were recorded on demand for three days using KM portable device. Each KM ECG record was verified by a cardiologist. The feasibility was evaluated using operationalization criteria. (3) Results: the recruitment rate among AIS patients was 26.3%. The withdrawal rate before the start of the intervention was 26%. The withdrawal rate after the start of the intervention was 6%. KM device detected AF in 2.8% of AIS patients and in 2.2% of ECG records. Cardiologist confirmed the AF in 0.3% AIS patients. Sensitivity and specificity of KM for AF was 100% and 98.3%, respectively. (4) Conclusions: the results of this study suggest that it is feasible to use KM device to detect AF in the selected AIS patients (younger and in better neurological condition). KM detected AF in the selected AIS patients with high specificity and sensitivity.
The Paris System for Reporting Urinary Cytology: A Meta-Analysis
Authors: Nikas Ilias P. , Seide Svenja , Proctor Tanja , Kleinaki Zoi , Kleinaki Maria , Reynolds Jordan P.
Published: January 2022

The Paris System (TPS) for Reporting Urinary Cytology is a standardized, evidence-based reporting system, comprising seven diagnostic categories: nondiagnostic, negative for high-grade urothelial carcinoma (NHGUC), atypical urothelial cells (AUC), suspicious for high-grade urothelial carcinoma (SHGUC), HGUC, low-grade urothelial neoplasm (LGUN), and other malignancies. This study aimed to calculate the pooled risk of high-grade malignancy (ROHM) of each category and demonstrate the diagnostic accuracy of urine cytology reported with TPS. Four databases (PubMed, Embase, Scopus, Web of Science) were searched. Specific inclusion and exclusion criteria were applied, while data were extracted and analyzed both qualitatively and quantitatively. The pooled ROHM was 17.70% for the nondiagnostic category (95% CI, 0.0650; 0.3997), 13.04% for the NHGUC (95% CI, 0.0932; 0.1796), 38.65% for the AUC (95% CI, 0.3042; 0.4759), 12.45% for the LGUN (95% CI, 0.0431; 0.3101), 76.89 for the SHGUC (95% CI, 0.7063; 0.8216), and 91.79% for the HGUC and other malignancies (95% CI, 0.8722; 0.9482). A summary ROC curve was created and the Area Under the Curve (AUC) was 0.849, while the pooled sensitivity was 0.669 (95% CI, 0.589; 0.741) and false-positive rate was 0.101 (95% CI, 0.063; 0.158). In addition, the pooled DOR of the included studies was 21.258 (95% CI, 14.336; 31.522). TPS assigns each sample into a diagnostic category linked with a specific ROHM, guiding clinical management.
Self-Reported Social Relationship Capacities Predict Motor, Functional and Cognitive Decline in Huntington’s Disease
Authors: Lemercier Pablo , Cleret de Langavant Laurent , Hamet Bagnou Jennifer , Youssov Katia , Lemoine Laurie , Audureau Etienne , Massart Renaud , Bachoud-Lévi Anne-Catherine
Published: January 2022

Huntington’s Disease (HD) is an inherited neurodegenerative disease characterized by a combination of motor, cognitive, and behavioral disorders. The social and behavioral symptoms observed in HD patients impact their quality of life and probably explain their relational difficulties, conflicts, and social withdrawal. In this study, we described the development of the Social Relationship Self-Questionnaire (SRSQ), a self-reporting questionnaire that assesses how HD patients perceived their social relationships. The scale was proposed for 66 HD patients at an early stage of the disease, 32 PreHD patients (individuals carrying the mutant gene without motor symptoms), and 66 controls. The HD patients were included in a prospective longitudinal follow-up for an average of 1.07 years with motor, functional, cognitive, and behavioral assessments. Based on the HD patients’ answers at baseline, we identified two domains in the SRSQ. The first domain was related to social motivation and correlated with cognitive performance. The second domain was related to emotional insight and correlated with behavioral symptoms such as apathy, anxiety, and irritability. We discovered that both SRSQ domain scores at baseline predicted future motor, functional, and cognitive decline in HD.
Retinal Disease and Metabolism
Authors: Fu Zhongjie , Usui-Ouchi Ayumi , Allen William , Tomita Yohei
Published: January 2022

Retinal diseases, such as diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinopathy of prematurity (ROP), are some of the leading causes of blindness all over the world [...]
Identification, Culture and Targeting of Cancer Stem Cells
Authors: Herreros-Pomares Alejandro
Published: January 2022

Chemoresistance, tumor progression, and metastasis are features that are frequently seen in cancer that have been associated with cancer stem cells (CSCs). These cells are a promising target in the future of cancer therapy but remain largely unknown. Deregulation of pathways that govern stemness in non-tumorigenic stem cells (SCs), such as Notch, Wnt, and Hedgehog pathways, has been described in CSC pathogenesis, but it is necessary to conduct further studies to discover potential new therapeutic targets. In addition, some markers for the identification and characterization of CSCs have been suggested, but the search for specific CSC markers in many cancer types is still under development. In addition, methods for CSC cultivation are also under development, with great heterogeneity existing in the protocols used. This review focuses on the most recent aspects of the identification, characterization, cultivation, and targeting of human CSCs, highlighting the advances achieved in the clinical implementation of therapies targeting CSCs and remarking those potential areas where more research is still required.
Visceral Leishmaniasis Associated with B-Cell Chronic Lymphocytic Leukemia: Report of a Case and Review of the Literature
Authors: Zanelli Magda , Tafuni Alessandro , Sanguedolce Francesca , Zizzo Maurizio , Palicelli Andrea , Simonetti Edoardo , Scarpelli Nando , Quintini Martina , Rosignoli Daniele , Grasselli Sara , Cavazza Alberto , Martino Giovanni , Ascani Stefano
Published: January 2022

Infections often complicate the course of hematological diseases and may represent a diagnostic challenge. In particular, visceral leishmaniasis diagnosis may be missed in lymphoma patients, as lymphoma-related immunosuppression can lead to a misleadingly negative Leishmania serology and to atypical clinical manifestations, including the lack of fever, considered a common symptom in leishmaniasis. Herein, we report a case of visceral leishmaniasis in a patient with a long history of B-cell chronic lymphocytic leukemia presenting with increasing fatigue and diarrhea, in the absence of fever. Leishmania serology was negative. Bone marrow biopsy performed with the clinical suspicion of transformation to high-grade lymphoma disclosed intracytoplasmic inclusion bodies resembling Leishmania amastigotes within the cytoplasm of macrophages, and CD1a immunohistochemical expression helped to confirm the diagnosis of leishmaniasis. Liposomal amphotericin B was administered with complete symptom resolution. The correct identification of Leishmania is critical as visceral leishmaniasis represents a severe disease with an often fatal outcome, particularly in frail patients, unless promptly recognized and adequately treated. A review of the literature of visceral leishmaniasis cases occurring in B-cell chronic lymphocytic leukemia patients is performed.
Accurate Quantitative Histomorphometric-Mathematical Image Analysis Methodology of Rodent Testicular Tissue and Its Possible Future Research Perspectives in Andrology and Reproductive Medicine
Authors: Sziva Réka Eszter , Ács Júlia , Tőkés Anna-Mária , Korsós-Novák Ágnes , Nádasy György L. , Ács Nándor , Horváth Péter Gábor , Szabó Anett , Ke Haoran , Horváth Eszter Mária , Kopa Zsolt , Várbíró Szabolcs
Published: January 2022

Infertility is increasing worldwide; male factors can be identified in nearly half of all infertile couples. Histopathologic evaluation of testicular tissue can provide valuable information about infertility; however, several different evaluation methods and semi-quantitative score systems exist. Our goal was to describe a new, accurate and easy-to-use quantitative computer-based histomorphometric-mathematical image analysis methodology for the analysis of testicular tissue. On digitized, original hematoxylin-eosin (HE)-stained slides (scanned by slide-scanner), quantitatively describable characteristics such as area, perimeter and diameter of testis cross-sections and of individual tubules were measured with the help of continuous magnification. Immunohistochemically (IHC)-stained slides were digitized with a microscope-coupled camera, and IHC-staining intensity measurements on digitized images were also taken. Suggested methods are presented with mathematical equations, step-by-step detailed characterization and representative images are given. Our novel quantitative histomorphometric-mathematical image analysis method can improve the reproducibility, objectivity, quality and comparability of andrological-reproductive medicine research by recognizing even the mild impairments of the testicular structure expressed numerically, which might not be detected with the present semi-quantitative score systems. The technique is apt to be subjected to further automation with machine learning and artificial intelligence and can be named ‘Computer-Assisted or -Aided Testis Histology’ (CATHI).
Abdominal Aortic Aneurysm Formation with a Focus on Vascular Smooth Muscle Cells
Authors: Qian Guoqing , Adeyanju Oluwaseun , Olajuyin Ayobami , Guo Xia
Published: January 2022

Abdominal aortic aneurysm (AAA) is a lethal degenerative vascular disease that affects, mostly, the elder population, with a high mortality rate (>80%) upon rupture. It features a dilation of the aortic diameter to larger than 30 mm or more than 50%. Diverse pathological processes are involved in the development of AAA, including aortic wall inflammation, elastin breakdown, oxidative stress, smooth muscle cell (SMC) phenotypic switching and dysfunction, and extracellular matrix degradation. With open surgery being the only therapeutic option up to date, the lack of pharmaceutical treatment approach calls for identifying novel and effective targets and further understanding the pathological process of AAA. Both lifestyle and genetic predisposition have an important role in increasing the risk of AAA. Several cell types are closely related to the pathogenesis of AAA. Among them, vascular SMCs (VSMCs) are gaining much attention as a critical contributor for AAA initiation and/or progression. In this review, we summarize what is known about AAA, including the risk factors, the pathophysiology, and the established animal models of AAA. In particular, we focus on the VSMC phenotypic switching and dysfunction in AAA formation. Further understanding the regulation of VSMC phenotypic changes may provide novel therapeutic targets for the treatment or prevention of AAA.
Corrosion Effects on Fracture Toughness Properties of Wire Arc Additively Manufactured Low Carbon Steel Specimens
Authors: Ermakova Anna , Mehmanparast Ali
Published: January 2022

The emerging wire + arc additive-manufacturing (WAAM) technique has significant potential to improve material design, as well as manufacturing cost and efficiency of structural components such as offshore wind turbines and subsequently reduce the levelised cost of energy (LCoE). Welded joints in offshore structures are usually considered potential spots for crack initiation due to the combination of high stress concentration at the weld toes, residual stresses introduced by the welding process and cyclic loading conditions in harsh, corrosive marine environments. The WAAM technique is a deposition method consisting of repetitive welding process that can be used as an alternative manufacturing technique for fabrication or repair of structural components. An important issue that needs to be understood in structural-integrity assessment of WAAM-built components is fracture-toughness behaviour. In particular, the sensitivity of fracture-toughness properties to corrosive environments must be examined in order to extend the application of the WAAM technique to offshore wind structures. Therefore, in this study, fracture-toughness tests were conducted on WAAM-built compact-tension specimens made of ER70S-6 and ER100S-1 steel that were initially exposed to a seawater corrosive environment prior to testing. All fracture-toughness tests were performed at room temperature, and crack length was estimated using the compliance method with a clip gauge attached onto the knife edge of the specimens. The obtained results, which include load vs. load-line displacement and J-integral vs. crack extension, were analysed and compared with the results of tests in air, without any exposure to seawater. The conclusions of this study show that corrosive environments affect the yield stress and R-curves of the selected materials and contribute to the overall understanding of the design requirements for functionally graded structures fabricated by means of WAAM technique for offshore applications.
Microstructure and Mechanical Properties of Laser Additive Manufactured H13 Tool Steel
Authors: Trojan Karel , Ocelík Václav , Čapek Jiří , Čech Jaroslav , Canelo-Yubero David , Ganev Nikolaj , Kolařík Kamil , De Hosson Jeff T. M.
Published: January 2022

Hot working tool steel (AISI H13) is one of the most common die materials used in casting industries. A die suffers from damage due to friction and wear during its lifetime. Therefore, various methods have been developed for its repair to save costs to manufacture a new one. A great benefit of laser additive manufacturing (cladding) is the 3D high production rate with minimal influence of thermal stresses in comparison with conventional arc methods. Residual stresses are important factors that influence the performance of the product, especially fatigue life. Therefore, the aim of this contribution is to correlate the wide range of results for multilayer cladding of H13 tool steel. X-ray and neutron diffraction experiments were performed to fully describe the residual stresses generated during cladding. Additionally, in-situ tensile testing experiments inside a scanning electron microscope were performed to observe microstructural changes during deformation. The results were compared with local hardness and wear measurements. Because laser cladding does not achieve adequate accuracy, the effect of necessary post-grinding was investigated. According to the findings, the overlapping of beads and their mutual tempering significantly affect the mechanical properties. Further, the outer surface layer, which showed tensile surface residual stresses and cracks, was removed by grinding and surface compressive residual stresses were described on the ground surface.
Mechanism Assay of Honeysuckle for Heat-Clearing Based on Metabolites and Metabolomics
Authors: Wang Hechen , Tian Lu , Han Yiman , Ma Xiaoyao , Hou Yuanyau , Bai Gang
Published: January 2022

Nonsteroidal anti-inflammatory drugs (NSAIDs), such as cyclooxygenase (Cox)-1/2 inhibitor, have emerged as potent antipyretics and analgesics. However, few herbs with Cox-1/2 inhibitory activity are commonly used for heat-clearing in China. Although these are known to have antipyretic activity, there is a lack of molecular data supporting their activity. Using the traditional Chinese medicine herb honeysuckle (Hon) as an example, we explored key antipyretic active compounds and their mechanisms of action by assessing their metabolites and metabolomics. Mitogen-activated protein kinase (MAPK) 3 and protein kinase B (AKT) 1 were suggested as key targets regulated primarily by chlorogenic acid (CA) and swertiamarin (SWE). CA and SWE synergistically inhibited the production of interleukin (IL)-1 and IL-6, alleviated generation of prostaglandin E2, and played an antipyretic role equivalent to honeysuckle extract at the same dose contents within 3 h. Collectively, these findings indicated that lipopolysaccharide-induced fever can be countered by CA with SWE synergistically, allowing the substitution of a crude extract of complex composition with active compounds. Our findings demonstrated that, unlike the traditional NSAIDs, the Hon extract showed a remote and indirect mechanism for alleviating fever that depended on the phosphatidylinositol-3-kinase–AKT and MAPK pathways by regulating the principal mediator of inflammation.