bluuebunny/crossref_metadata_2025 huggingface dataset:
Innovating digital POE platforms during the COVID-19 pandemic: A case study of co-production in Brazilian social housing
Authors: Villa Simone Barbosa , Stevenson Fionn , Garrefa Fernando , de Bortoli Karen Carrer Ruman , Lya de Melo Carvalho Nathalia
Published: February 2023

This paper deals with digital POE methods focusing on their reached potential in facilitating the face-to-face experiments demanded by the project. Recognized as a set of methods and instruments applied in environments during use, Post-Occupancy Evaluation (POE) can help manage the project process, providing diagnoses and recommendations that feed new projects and interventions in environments in use. POE approaches have been continually restructured in the face of new environmental, social, economic and especially technological demands. In early 2020, the Covid-19 pandemic, lockdowns and quarantines made it challenging to continue to apply traditional face-to-face POE-based tools and techniques. Simultaneously, the MORA Housing Research Group was carrying out a research project in the city of Uberlândia (Brazil) focusing on the understanding of the relationship between the incidence of arboviruses in the case study social housing neighbourhood, the occupants’ practices, and the built environment management, through POE. The social distancing measures applied during the Covid-19 pandemic led the research team to transform methods from face-to-face to hybrid ones, comprising digital and physical approaches to co-produce durable solutions against dengue. The work comprises a five stage method using on-shelf digital platforms and designing other 2 specifically for the project’s purposes. By introducing the digital/physical hybrid POE format, the use of social networks and messaging applications significantly reduced the number of necessary visits by researchers to the neighbourhood from 20 to 5 in the first stage of the work, saving a lot of time and effort. The new tools were easy to use for all users. Users reported finding it pleasant to participate in activities with digitally remote researchers. The findings from this project indicate that the incorporation of new digital tools to facilitate physical POE research as a novel hybrid approach provides a successful alternative approach to standard POE processes that are based on face-to-face participation.
Cerebral amyloid angiopathy-related cardiac injury: Focus on cardiac cell death
Authors: Xu Xiaofang , Xu Huikang , Zhang Zhaocai
Published: February 2023

Cerebral amyloid angiopathy (CAA) is a kind of disease in which amyloid β (Aβ) and other amyloid protein deposits in the cerebral cortex and the small blood vessels of the brain, causing cerebrovascular and brain parenchymal damage. CAA patients are often accompanied by cardiac injury, involving Aβ, tau and transthyroxine amyloid (ATTR). Aβ is the main injury factor of CAA, which can accelerate the formation of coronary artery atherosclerosis, aortic valve osteogenesis calcification and cardiomyocytes basophilic degeneration. In the early stage of CAA (pre-stroke), the accompanying locus coeruleus (LC) amyloidosis, vasculitis and circulating Aβ will induce first hit to the heart. When the CAA progresses to an advanced stage and causes a cerebral hemorrhage, the hemorrhage leads to autonomic nervous function disturbance, catecholamine surges, and systemic inflammation reaction, which can deal the second hit to the heart. Based on the brain-heart axis, CAA and its associated cardiac injury can create a vicious cycle that accelerates the progression of each other.
Interactions between microbiota and cervical epithelial, immune, and mucus barrier
Authors: Dong Mengting , Dong Yalan , Bai Junyi , Li Huanrong , Ma Xiaotong , Li Bijun , Wang Chen , Li Huiyang , Qi Wenhui , Wang Yingmei , Fan Aiping , Han Cha , Xue Fengxia
Published: February 2023

The female reproductive tract harbours hundreds of bacterial species and produces numerous metabolites. The uterine cervix is located between the upper and lower parts of the female genital tract. It allows sperm and birth passage and hinders the upward movement of microorganisms into a relatively sterile uterus. It is also the predicted site for sexually transmitted infection (STI), such as Chlamydia, human papilloma virus (HPV), and human immunodeficiency virus (HIV). The healthy cervicovaginal microbiota maintains cervical epithelial barrier integrity and modulates the mucosal immune system. Perturbations of the microbiota composition accompany changes in microbial metabolites that induce local inflammation, damage the cervical epithelial and immune barrier, and increase susceptibility to STI infection and relative disease progression. This review examined the intimate interactions between the cervicovaginal microbiota, relative metabolites, and the cervical epithelial-, immune-, and mucus barrier, and the potent effect of the host-microbiota interaction on specific STI infection. An improved understanding of cervicovaginal microbiota regulation on cervical microenvironment homeostasis might promote advances in diagnostic and therapeutic approaches for various STI diseases.
Stroke risk study based on deep learning-based magnetic resonance imaging carotid plaque automatic segmentation algorithm
Authors: Chen Ya-Fang , Chen Zhen-Jie , Lin You-Yu , Lin Zhi-Qiang , Chen Chun-Nuan , Yang Mei-Li , Zhang Jin-Yin , Li Yuan-zhe , Wang Yi , Huang Yin-Hui
Published: February 2023

Introduction

The primary factor for cardiovascular disease and upcoming cardiovascular events is atherosclerosis. Recently, carotid plaque texture, as observed on ultrasonography, is varied and difficult to classify with the human eye due to substantial inter-observer variability. High-resolution magnetic resonance (MR) plaque imaging offers naturally superior soft tissue contrasts to computed tomography (CT) and ultrasonography, and combining different contrast weightings may provide more useful information. Radiation freeness and operator independence are two additional benefits of M RI. However, other than preliminary research on MR texture analysis of basilar artery plaque, there is currently no information addressing MR radiomics on the carotid plaque.

Methods

For the automatic segmentation of MRI scans to detect carotid plaque for stroke risk assessment, there is a need for a computer-aided autonomous framework to classify MRI scans automatically. We used to detect carotid plaque from MRI scans for stroke risk assessment pre-trained models, fine-tuned them, and adjusted hyperparameters according to our problem.

Results

Our trained YOLO V3 model achieved 94.81% accuracy, RCNN achieved 92.53% accuracy, and MobileNet achieved 90.23% in identifying carotid plaque from MRI scans for stroke risk assessment. Our approach will prevent incorrect diagnoses brought on by poor image quality and personal experience.

Conclusion

The evaluations in this work have demonstrated that this methodology produces acceptable results for classifying magnetic resonance imaging (MRI) data.
Variability of trace-elements and δ18O in drip water from Gruta del Rey Marcos, Guatemala; seasonal and environmental effects, and its implications for paleoclimate reconstructions
Authors: Bernal J. P. , Revolorio F. , Cu-Xi M. , Lases-Hernández F. , Piacsek P. , Lachniet Matthew S. , Beddows Patrica A. , Lucia G. , López-Aguiar K. , Capella-Vizcaíno S. , López-Martínez R. , Vásquez O. J.
Published: February 2023

Guatemala is located at the core of one of the largest warming pools in the planet, the Western Hemisphere Warm Pool, an important source of tropical moisture to middle and high latitudes and, thus, a key area for paleoclimatic studies. This, along the karst pervasiveness in the area provides the opportunity to obtain high-resolution records of past hydroclimatic conditions using stalagmites. Despite this, the atmospheric and geochemical processes that might affect the variability of geochemical proxies in stalagmites are yet to be constrained, as no cave-monitoring in the area has been carried out previously. Here, we present a 2.5-year cave-monitoring study from Gruta del Rey Marcos, Guatemala, which allows to understand the effect of external atmospheric and environmental conditions upon the variability of δ18O, Mg/Ca, Sr/Ca, and Ba/Ca in drip water. By incorporating cave ventilation dynamics, isotopic information of local rainfall and cave-river water, we are able to understand the most relevant processes that affect the variability of the geochemical proxies in drip water, hence stalagmites. Our results suggest that two-isotopically distinct rainfall regimes, as well as tropical cyclones affecting the area contribute to the composition of the drip-water, hence the resulting stalagmites, with low δ18O (∼-6‰ VSMOW) values indicative of strong convective activity, whilst high δ18O values (−4.5 and −5‰ VSMOW), are indicative of poor convection and proportionally more significant winter-frontal rainfall. We also demonstrate that the trace-element composition of drip water is largely modulated by PCP, and thus, the variability in underlying stalagmites can be interpreted to reflect changes in karst humidity, with low E/Ca ratios (E = Mg, Sr, Ba), indicative of a wet epikarst and high E/Ca ratios indicative of a dry epikarst. Our results provide essential information for the interpretation of the δ18O and trace element variability in stalagmites, which can result in robust paleoclimatic reconstructions from Gruta del Rey Marcos and elsewhere in central America and southern Mexico.
Understanding the delayed Amundsen Sea low response to ENSO
Authors: Lee Hyun-Ju , Jin Emilia Kyung
Published: February 2023

Although the El Niño-Southern Oscillation (ENSO) affects West Antarctica via teleconnection, it is delayed by a season, because the Amundsen Sea Low (ASL) anomaly in response to the ENSO is the strongest in May. However, the process and mechanism of the delay has not been fully elucidated yet. In this study, we examined the formation of the ENSO-driven teleconnection in each month from January to May by analyzing the kinetic energy conversion and Rossby wave propagation. The flow perturbed by the ENSO gains energy from the basic state by energy conversion, but the perturbation does not reach the high latitudes until April. In May, although the ENSO intensity becomes weak, the development of the subtropical jet induces waves to propagate further south, resulting in the anticyclonic circulation anomaly over the ASL region. Numerical experiments that account for the decay of the ENSO forcing and the monthly varying basic state also indicate that the formation of the teleconnection is the strongest in May. The results reveal that the configuration of the basic state is crucial for the teleconnection in response to the ENSO to reach West Antarctica.
The institutional approach to the development of education in the social and investment model of Russia's economic growth
Authors: Krupnov Yuriy A. , Yankovskaya Veronika V. , Semenova Elena I. , Semenov Aleksandr V.
Published: February 2023

The goal of this article is to develop an institutional approach to the development of education in the social and investment model of economic growth in developing countries by the example of Russia. The hypothetical and deductive principles and regression analysis are used in this research. The research results show that the social and investment model of economic growth is being implemented in the countries of the CIS and Russia, and—as of now—the development of education slows down economic growth, instead of accelerating it. To solve this problem, we offer an institutional approach to the development of education in the social and investment model of Russia's economic growth. The new approach allows for a flexible selection of measures of management depending on the needs of education's development: development of only current, only future, or all workforce. The developed approach is based on the institution of the legal framework's adaptability to digital business models. Since workforce and skills play a key supporting role in digital business, the development of the institution of legal framework's adaptability to digital business models will stimulate the development of higher education and will ensure Russia's transition to the social and investment model of economic growth. For the practical application of the new approach, we develop an optimization model of institutional management in the interests of the development of education and transition to the social and investment model of economic growth in Russia. The model has shown that an increase in the possibility of the legal framework's adaptability to digital business models up to 100 points with a stable level of development of other institutions allows increasing skills of the current workforce by 44.05% and the future workforce by 20.96%.
High hemoglobin glycation index is associated with increased risk of diabetes: A population-based cohort study in China
Authors: Lin Lu , Wang Anping , Jia Xiaomeng , Wang Haibin , He Yan , Mu Yiming , Dou Jingtao
Published: February 2023

Purpose

The hemoglobin glycation index (HGI) quantifies the mismatch between glycated hemoglobin A1c and average glycemia among individuals. Currently, it is unknown the potential role of HGI in exhaustively evaluating the progression of glucose metabolism/the risk of developing diabetes mellitus. Therefore, this study aimed to investigate the association between HGI and the risk of incident diabetes.

Methods

A total of 7,345 participants aged at least 40 years and without diabetes were divided into three groups according to the tertile of their baseline HGI level and followed for a median of 3.24 years to track new-onset diabetes. Using multivariate Cox regression analyses, we explored the association between the HGI, both categorized and continuous, and incident diabetes.

Results

During follow-up, 742 subjects (263 males and 479 females) developed diabetes mellitus. Higher HGI was associated with an increased risk of diabetes, even when adjusted for confounding factors, and every standard deviation increase in HGI was associated with a significant risk increase of 30.6% for diabetes (hazard ratio 1.306, 95% confidence interval 1.232–1.384).

Conclusions

Participants with a higher HGI were at a higher risk of future diabetes, irrespective of their glycemic conditions. Consequently, HGI may be employed to identify individuals at high risk for diabetes.
Molecular diagnostics in the evaluation of thyroid nodules: Current use and prospective opportunities
Authors: Patel Jena , Klopper Joshua , Cottrill Elizabeth E.
Published: February 2023

Thyroid cancer is the most common endocrine malignancy with an estimated 43,800 new cases to be diagnosed in 2022 and representing the 7th most common cancer in women. While thyroid nodules are very common, being identified in over 60% of randomly selected adults, only 5-15% of thyroid nodules harbor thyroid malignancy. Therefore, it is incumbent upon physicians to detect and treat thyroid malignancies as is clinically appropriate and avoid unnecessary invasive procedures in patients with benign asymptomatic lesions. Over the last 15-20 years, rapid advances have been made in cytomolecular testing to aid in thyroid nodule management. Initially, indeterminate thyroid nodules, those with Bethesda III or IV cytology and approximately a 10-40% risk of malignancy, were studied to assess benignity or malignancy. More recently, next generation sequencing and micro-RNA technology platforms have refined the diagnostic capacity of thyroid nodule molecular testing and have introduced opportunities to glean prognostic information from both cytologically indeterminate and malignant thyroid nodules. Therefore, clinicians can move beyond determination of malignancy, and utilize contemporary molecular information to aid in decisions such as extent of surgery and post-therapy monitoring plans. Future opportunities include molecularly derived information about tumor behavior, neo-adjuvant treatment opportunities and response to thyroid cancer therapies.
Which crop has the highest bioethanol yield in the United States?
Authors: Lin Tzu-Shun , Kheshgi Haroon S. , Song Yang , Vörösmarty Charles J. , Jain Atul K.
Published: February 2023

Annual U.S. production of bioethanol, primarily produced from corn starch in the U.S. Midwest, rose to 57 billion liters in 2021, which fulfilled the required conventional biofuel target set forth by the Energy Independence and Security Act (EISA) of 2007. At the same time, the U.S. fell short of the cellulosic or advanced biofuel target of 79 billion liters. The growth of bioenergy grasses (e.g., Miscanthus and switchgrass) across the Central and Eastern U.S. has the potential to feed enhanced cellulosic bioethanol production and, if successful, increase renewable fuel volumes. However, water consumption and climate change and its extremes are critical concerns in corn and bioenergy grass productivity. These concerns are compounded by the demands on potentially productive land areas and water devoted to producing biofuels. This is a fundamental Food-Energy-Water System (FEWS) nexus challenge. We apply a computational framework to estimate potential bioenergy yield and conversion to bioethanol yield across the U.S., based on crop field studies and conversion technology analysis for three crops—corn, Miscanthus, and two cultivars of switchgrass (Cave-in-Rock and Alamo). The current study identifies regions where each crop has its highest yield across the Center and Eastern U.S. While growing bioenergy grasses requires more water than corn, one advantage they have as a source of bioethanol is that they control nitrogen leaching relative to corn. Bioenergy grasses also maintain steadily high productivity under extreme climate conditions, such as drought and heatwaves in the year 2012 over the U.S. Midwest, because the perennial growing season and the deeper and denser roots can ameliorate the soil water stress. While the potential ethanol yield could be enhanced using energy grasses, their practical success in becoming a potential source of ethanol yield remains limited by socio-economic and operational constraints and concerns regarding competition with food production.
Why do people participate in app-based environment-focused citizen science projects?
Authors: Etter Simon , Strobl Barbara , Seibert Jan , van Meerveld H. J. (Ilja) , Niebert Kai , Stepenuck Kristine F.
Published: February 2023

We investigated the motivations of participants in two environment-focused citizen science projects using an online questionnaire. The questions focused on the reasons for initial engagement and in how far these motivations were fulfilled by participating. The two projects, CrowdWater and Naturkalender (English: Nature’s Calendar), use similar smartphone applications to collect data on water and phenology, respectively. The answers to the individual statements were analyzed based on a categorization framework that was previously used with other citizen science projects. The motivations to participate in the projects were similar for the two projects but there were also some differences. They were altruistic and related to participants’ principles (e.g., to uphold a moral principle, such as through conservation). The main motivations for becoming engaged in the projects were to contribute to science, due to an interest in the project topic, and to protect nature. More CrowdWater respondents were motivated by being asked to participate than Naturkalender respondents. Naturkalender participants and participants in the 50–59-year age group of both projects agreed most to enjoying their participation, being outside and active, and learning something new. More super-users, i.e., users who participated at least once per week, were interested in sharing their knowledge and experience with others than occasional participants. This was particularly true for super-users in Naturkalender. Based on the results of this study, we recommend that to help sustain involvement of the most active participants, projects should focus on recruiting participants who are already interested in the topic, and highlighting opportunities to share knowledge, be outdoors, and contribute to science.
Genome editing for improving nutritional quality, post-harvest shelf life and stress tolerance of fruits, vegetables, and ornamentals
Authors: Sharma Punam , Pandey Anuradha , Malviya Rinku , Dey Sharmistha , Karmakar Subhasis , Gayen Dipak
Published: February 2023

Agricultural production relies on horticultural crops, including vegetables, fruits, and ornamental plants, which sustain human life. With an alarming increase in human population and the consequential need for more food, it has become necessary for increased production to maintain food security. Conventional breeding has subsidized the development of improved verities but to enhance crop production, new breeding techniques need to be acquired. CRISPR-Cas9 system is a unique and powerful genome manipulation tool that can change the DNA in a precise way. Based on the bacterial adaptive immune system, this technique uses an endonuclease that creates double-stranded breaks (DSBs) at the target loci under the guidance of a single guide RNA. These DSBs can be repaired by a cellular repair mechanism that installs small insertion and deletion (indels) at the cut sites. When equated to alternate editing tools like ZFN, TALENs, and meganucleases, CRISPR- The cas-based editing tool has quickly gained fast-forward for its simplicity, ease to use, and low off-target effect. In numerous horticultural and industrial crops, the CRISPR technology has been successfully used to enhance stress tolerance, self-life, nutritional improvements, flavor, and metabolites. The CRISPR-based tool is the most appropriate one with the prospective goal of generating non-transgenic yields and avoiding the regulatory hurdles to release the modified crops into the market. Although several challenges for editing horticultural, industrial, and ornamental crops remain, this new novel nuclease, with its crop-specific application, makes it a dynamic tool for crop improvement.
Novel regulatory role of non-coding RNAs in ankylosing spondylitis
Authors: Fang Yanyan , Liu Jian
Published: February 2023

Ankylosing spondylitis (AS) is a type of arthritis that primarily affects the spine and involves disorders of the immune and skeletal systems. However, the exact pathogenesis of AS is not fully understood. Non-coding RNAs (ncRNAs), particularly, long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and micro RNAs (miRNAs) and their interactions have been shown to influence many biological processes such as inflammatory responses, osteogenic differentiation and apoptosis, pyroptosis, and proliferation. In addition, ncRNAs reflect the disease activity of AS. In this review, we discuss the regulatory roles of ncRNAs in AS cell functions (inflammatory responses, cellular osteogenic differentiation and apoptosis, pyroptosis, and proliferation) and their potential applications in AS diagnosis and treatment. Understanding the role of ncRNAs in the pathogenesis of AS will lay the foundation for exploring potential new therapeutic approaches for AS.
Mesenchymal stem cells in the treatment of spinal cord injury: Mechanisms, current advances and future challenges
Authors: Xia Yuanliang , Zhu Jianshu , Yang Ruohan , Wang Hengyi , Li Yuehong , Fu Changfeng
Published: February 2023

Spinal cord injury (SCI) has considerable impact on patient physical, mental, and financial health. Secondary SCI is associated with inflammation, vascular destruction, and subsequent permanent damage to the nervous system. Mesenchymal stem cells (MSCs) have anti-inflammatory properties, promoting vascular regeneration and the release neuro-nutrients, and are a promising strategy for the treatment of SCI. Preclinical studies have shown that MSCs promote sensory and motor function recovery in rats. In clinical trials, MSCs have been reported to improve the American Spinal Injury Association (ASIA) sensory and motor scores. However, the effectiveness of MSCs in treating patients with SCI remains controversial. MSCs promote tumorigenesis and ensuring the survival of MSCs in the hostile environment of SCI is challenging. In this article we examine the evidence on the pathophysiological changes occurring after SCI. We then review the underlying mechanisms of MSCs in the treatment of SCI and summarize the potential application of MSCs in clinical practice. Finally, we highlight the challenges surrounding the use of MSCs in the treatment of SCI and discuss future applications.
Origin and driving mechanisms of marine litter in the shelf-incised Motril, Carchuna, and Calahonda canyons (northern Alboran Sea)
Authors: Cerrillo-Escoriza Javier , Lobo Francisco José , Puga-Bernabéu Ángel , Rueda José Luis , Bárcenas Patricia , Sánchez-Guillamón Olga , Serna Quintero José Miguel , Pérez Gil José Luis , Murillo Yelvana , Caballero-Herrera José Antonio , López-Quirós Adrián , Mendes Isabel , Pérez-Asensio José Noel
Published: February 2023

Introduction and methods

Marine litter density, distribution and potential sources, and the impact on canyon seafloor habitats were investigated in the Motril, Carchuna and Calahonda canyons, located along the northern margin of the Alboran Sea. During the ALSSOMAR-S2S oceanographic survey carried out in 2019, canyon floor imagery was collected by a Remotely Operated Vehicle along 5 km in the Motril Canyon, 10 km in the Carchuna Canyon, and 3 km in Calahonda Canyon, together with 41 surficial sediment samples. Additionally, coastal uses, maritime traffic and fishing activity data were analyzed. A 50 m resolution multibeam bathymetry served as base map.

Results

In the Motril and Calahonda canyons, the density of marine litter was low and the material was dispersed, very degraded and partially buried. In contrast, the Carchuna Canyon contained a greater amount and variety of litter. The Carchuna Canyon thalweg exhibited a density of marine litter up to 8.66 items·100 m-1, and litter hotspots with a density of up to 42 items·m2 are found along the upper reaches of the canyon thalweg.

Discussion

Low litter abundances found in the studied canyons most likely reflect low population densities and the absence of direct connections with streams in the nearby coasts. The high shelf incision of the Carchuna Canyon and its proximity to the coastline favor littoral sediment remobilization and capture as well as the formation of gravity flows that transport the marine litter along the thalweg toward the distal termination of the channel. Litter hotspots are favored by the canyon morphology and the occurrence of rocky outcrops. Most debris is of coastal origin and related to beach occupation and agricultural practices in the adjacent coastal plain. A third origin was represented by fishing gear in the study area. Fishing activity may be producing an impact through physical damage to the skeletons of the colonial scleractinians located in the walls of the Carchuna Canyon. In contrast, the Motril and Calahonda canyons can be considered passive systems that have mainly acted as depositional sinks in the recent past, as evidenced by buried marine litter.
The health-related quality of life for hemiarthroplasty and total hip arthroplasty in the elderly: A meta-analysis
Authors: Su Yaping , Li Ruiling , Ren Xiangying , Wang Yuanyuan , Bai Xiaolu , Zhang Yurui , Han Lingyu , Wang Yiman , Liang Ran
Published: February 2023

Purpose

What constitutes the optimal surgical plan for femoral neck fractures (FNFs) in elderly patients is controversial. The European quality of life 5-Dimension Questionnaire (EU-5Q) is an international scale used to measure the health-related quality of life (HRQoL) after surgery. We aim to verify the hip arthroplasty effect in elderly patients by analyzing HRQoL scores in the EU-5Q scale.

Methods

We searched the EBSCO, Embase, PubMed, Ovid, Cochrane Library, and Web of Science databases using strict searching from established to 30 November 2022; used the Cochrane Library's Risk of Bias Assessment Tool and the Newcastle–Ottawa Scale to evaluate the literature; and used RevMan5.4.1 software to perform a meta-analysis. All the included studies used the EU-5Q scale to validate the overall outcomes for elderly hip arthroplasty.

Results

The final included literature is composed of four RCTs, two cohort studies, three case–control trials, and three cross-sectional surveys. This study compared HRQoL scores measured by the EU-5Q scale, including 328 elderly patients with total hip arthroplasty (THA) and 323 elderly patients with hemiarthroplasty, which is statistically significant (OR = 0.05; 95% CI, 0.02~0.08; P = 0.002). The subgroups were as follows: unipolar vs. bipolar and cemented vs. uncemented hemiarthroplasty (OR = 0.06; 95% CI, 0.03~0.08; P < 0.001), follow-up time and age arthroplasty (OR = 0.16; 95% CI, 0.11~0.22; P < 0.001), molecular exercise and enhanced recovery after surgery (ERAS) (OR = 0.02; 95% CI,−0.03~0.07; P = 0.38), and analysis of hemiarthroplasty with cognitive dysfunction vs. the normal group (OR = 0.17; 95% CI, 0.08~0.26; P < 0.001). The outcome analysis was consistent with the included studies, and HRQoL of the EU-5Q scale is sensitive to surgical outcomes between THA and hemiarthroplasty.

Conclusion

Surgeons still need to further evaluate and verify whether the hip arthroplasty surgical program or effect in elderly patients is optimal. Hemiarthroplasty operations in elderly patients have pointed toward a new direction for clinical treatment, and HRQoL scores measured by the EU-5Q can sensitively reflect the rehabilitation status after hip arthroplasty surgery. Moreover, the extensive correlation between surgical outcomes and perioperative neurocognitive function should be further investigated.
A meta-analysis to observe silage microbiome differentiated by the use of inoculant and type of raw material
Authors: Ridwan Roni , Abdelbagi Mohammed , Sofyan Ahmad , Fidriyanto Rusli , Astuti Wulansih D. , Fitri Ainissya , Sholikin Mohammad M. , Rohmatussolihat  , Sarwono Ki A. , Jayanegara Anuraga , Widyastuti Yantyati
Published: February 2023

Silage fermentation is naturally carried out by lactic acid bacteria (LAB) to mainly produce lactic acid (LA) and other organic acids as preservatives. Along with fermentation time, the growth of LAB will replace and suppress undesirable microorganisms. This meta-analysis study aimed to explore silage microbiome differentiated by LAB inoculants and type of raw materials. A total of 37 articles with 185 studies and 475 datasets were used for building up the meta-database. Data were subjected to the mixed model methodology. The parameters observed were silage quality and silage microbiome post-ensiling process. Results revealed that four bacterial genera along with Weissella dominated the post-ensiling process. The addition of lactic acid inoculants in the silage has increased the abundance of Lactobacillus spp. and decreased the Shannon index significantly. Moreover, the abundance of both L. plantarum and L. buchneri increased, and subsequently, Weissella, Pseudomonas, Proteobacteria, pH value, ammoniacal nitrogen (NH3-N), coliforms, and the yeasts were decreased significantly due to the addition of LAB inoculants in silage (p < 0.05). Environmental factors such as temperature affected the existence of Pseudomonas, Exiguobacterium, and Acinetobacter. However, the dry matter, LA, acetic acid (AA), the ratio of LA to AA, and the LAB population were enhanced significantly (p < 0.05). Among the LAB types, the lowest abundance of Pseudomonas was due to the LAB group, while the lowest abundance of Weissella and Proteobacteria was due to the addition of the combined LAB group. In conclusion, the addition of LAB is effectively enhancing the silage microbiome and silage quality by altering bacterial diversity and the metabolic products of the silage materials for safe preservation.
Revealing within-species diversity in uncultured human gut bacteria with single-cell long-read sequencing
Authors: Kogawa Masato , Nishikawa Yohei , Saeki Tatsuya , Yoda Takuya , Arikawa Koji , Takeyama Haruko , Hosokawa Masahito
Published: February 2023

Obtaining complete and accurate bacterial genomes is vital for studying the characteristics of uncultured bacteria. Single-cell genomics is a promising approach for the culture-independent recovery of bacterial genomes from individual cells. However, single-amplified genomes (SAGs) often have fragmented and incomplete sequences due to chimeric and biased sequences introduced during the genome amplification process. To address this, we developed a single-cell amplified genome long-read assembly (scALA) workflow to construct complete circular SAGs (cSAGs) from long-read single-cell sequencing data of uncultured bacteria. We used the SAG-gel platform, which is both cost-effective and high-throughput, to obtain hundreds of short-read and long-read sequencing data for specific bacterial strains. The scALA workflow generated cSAGs by repeated in silico processing for sequence bias reduction and contig assembly. From 12 human fecal samples, including two cohabitant groups, scALA generated 16 cSAGs of three specifically targeted bacterial species: Anaerostipes hadrus, Agathobacter rectalis, and Ruminococcus gnavus. We discovered strain-specific structural variations shared among cohabiting hosts, while all cSAGs of the same species showed high homology in aligned genomic regions. A. hadrus cSAGs exhibited 10 kbp-long phage insertions, various saccharide metabolic capabilities, and different CRISPR-Cas systems in each strain. The sequence similarity of A. hadrus genomes did not necessarily correspond with orthologous functional genes, while host geographical regionality seemed to be highly related to gene possession. scALA allowed us to obtain closed circular genomes of specifically targeted bacteria from human microbiota samples, leading to an understanding of within-species diversities, including structural variations and linking mobile genetic elements, such as phages, to hosts. These analyses provide insight into microbial evolution, the adaptation of the community to environmental changes, and interactions with hosts. cSAGs constructed using this method can expand bacterial genome databases and our understanding of within-species diversities in uncultured bacteria.
Protein kinase C mediates hypoxia-induced long-term potentiation of NMDA neurotransmission in the visual retinocollicular pathway
Authors: Dumanska Hanna , Veselovsky Nikolai
Published: February 2023

The identification of processes and mechanisms underlying the early stage of hypoxic injury of the retinocollicular pathway may be beneficial for the future prevention and treatment of navigation, orientation, and visual attention impairments. Previously, we have demonstrated that short-term hypoxia led to long-term potentiation (LTP) of NMDA neurotransmission in the background of long-term depression of GABAA retinocollicular transmission. Here, we sought to obtain insight into the mechanisms of hypoxia-induced LTP of NMDA retinocollicular neurotransmission and the role of the protein kinase C (PKC) signaling pathway in it. To investigate these, we recorded pharmacologically isolated NMDA transmission in cocultivated pairs of rat retinal ganglion cells and superficial superior colliculus neurons under normoxic and hypoxic conditions, using the paired patch-clamp technique and method of fast local superfusion. We tested the involvement of the PKC by adding the potent and selective inhibitor chelerythrine chloride (ChC, 5 μM). We observed that hypoxia-induced LTP of NMDA neurotransmission is associated with the shortening of current kinetics. We also found that the PKC signaling pathway mediates hypoxia-induced LTP and associated shortening of NMDA currents. The ChC completely blocked the induction of LTP by hypoxia and associated kinetic changes. Contrary effects of ChC were observed with already induced LTP. ChC led to the reversal of LTP to the initial synaptic strength but the current kinetics remain irreversibly shortened. Our results show that ChC is a promising agent for the prevention and treatment of hypoxic injuries of NMDA retinocollicular neurotransmission and provide necessary electrophysiological basics for further research.
The need for kidney biopsy in the management of side effects of target and immunotherapy
Authors: Fenoglio Roberta , Cozzi Martina , Del Vecchio Giulio , Sciascia Savino , Barreca Antonella , Comandone Alessandro , Roccatello Dario
Published: February 2023

Introduction

The introduction of innovative therapies, resulting from revisiting cancer as a disease of the immune system, has changed the scenario of complications. These new classes of drugs, such as targeted therapies and immune checkpoint inhibitors, assure substantial advantages in cancer therapy, despite some side effects affecting various organs, including the kidney. Histological evaluations of kidney disorders induced by targeted/immunotherapy are limited.

Method

In this study we examined the histological features of patients treated with new cancer agents who underwent a kidney biopsy for new onset kidney failure and/or urinary abnormalities.

Results

The cohort included 30 adult patients. The most frequently administered therapies were immunotherapy (30%), targeted therapy (26.7%), immunotherapy plus targeted therapy (13.3%), immunotherapy plus chemotherapy (13.3%), targeted therapy plus chemotherapy (16.7%). The most common histological finding was tubular interstitial nephritis (30%) that was associated with acute tubular necrosis in 4 cases, and thrombotic microangiopathy (23.3%). After kidney biopsy, 16 of the 30 patients were treated according to the histological diagnosis. Fourteen patients were treated with steroids. One patient with membranous nephropathy was treated with a single dose of rituximab. A patient with severe thrombotic microangiopathy requiring dialysis received a treatment with eculizumab for 3 months. Overall some renal response was obtained in all patients treated with glucocorticoids, while complete kidney response was achieved in the patient treated with rituximab. Cancer treatment was resumed without change in 21 out of 30 patients.

Conclusion

Kidney biopsy is critical for the management of kidney toxicities and should be strongly encouraged for patients showing adverse kidney effects of novel cancer agents.
Fibrinogen-to-albumin ratio percentage: An independent predictor of disease severity and prognosis in anti-N-methyl-D-aspartate receptor encephalitis
Authors: Du Juan , Shao Yingzhe , Song Yajun , Wang Kaixin , Yang Xuan , Li Yanfei , Yao Yaobing , Gong Zhe , Jia Yanjie
Published: February 2023

Purpose

This retrospective study aimed to investigate the relationship between fibrinogen-to-albumin ratio percentage (FARP) and disease severity and prognosis in patients with anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis.

Methods

Medical records and clinical characteristics from 181 patients with anti-NMDAR encephalitis were included. The modified Rankin Scale (mRS) was used to analyze disease severity and prognosis at admission and discharge, and correlations between FARP, disease severity, and prognosis were analyzed. Receiver operating characteristic (ROC) curves were used to evaluate the efficiency of FARP in assessing disease severity and prognosis.

Results

Compared to the control group, patients with anti-NMDAR encephalitis had higher fibrinogen (Fib) levels (P < 0.001), neutrophil counts (P < 0.001), and FARP levels (P < 0.001) but had lower albumin levels (P = 0.003). The enrolled patients were divided into mild-to-moderate and severe groups according to their mRS scores both at admission and discharge. FARP levels were significantly elevated in the severe group compared to the mild-to-moderate group among patients with anti-NMDAR encephalitis both at admission and discharge (admission 6.0 vs. 7.40, P < 0.001; discharge 6.43 vs. 8.18, P<0.001). Indeed, the mRS scores at admission (56 vs. 26%, P < 0.001) and discharge (26 vs. 11%, P = 0.006) in the high FARP group were significantly higher than those in the low FARP group. Furthermore, FARP was positively correlated with the mRS scores at admission (r = 0.383, P < 0.001) and discharge (r =0.312, P < 0.001). In the multivariate analysis, FARP was significantly associated with disease severity (odds ratio [OR] = 1.416, 95% confidence interval [CI] = 1.117-1.795, P = 0.004) and prognosis (OR = 1.252, 95% CI = 1.010-1.552, P = 0.040). FARP-based ROC curves predicted disease severity, with a sensitivity of 0.756, a specificity of 0.626, and an area under the ROC curve of 0.722 (95% CI = 0.648–0.796, P < 0.001*). The ROC curve predicted the disease prognosis with a sensitivity of 0.703, a specificity of 0.667, and an area under the ROC curve of 0.723 (95% CI = 0.629–0.817, P < 0.001*).

Conclusion

Our results indicate that FARP is a novel predictive marker for disease severity and prognosis of anti-NMDAR encephalitis.
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome is common in post-acute sequelae of SARS-CoV-2 infection (PASC): Results from a post-COVID-19 multidisciplinary clinic
Authors: Bonilla Hector , Quach Tom C. , Tiwari Anushri , Bonilla Andres E. , Miglis Mitchell , Yang Phillip C. , Eggert Lauren E. , Sharifi Husham , Horomanski Audra , Subramanian Aruna , Smirnoff Liza , Simpson Norah , Halawi Houssan , Sum-ping Oliver , Kalinowski Agnieszka , Patel Zara M. , Shafer Robert William , Geng Linda N.
Published: February 2023

Background

The global prevalence of PASC is estimated to be present in 0·43 and based on the WHO estimation of 470 million worldwide COVID-19 infections, corresponds to around 200 million people experiencing long COVID symptoms. Despite this, its clinical features are not well-defined.

Methods

We collected retrospective data from 140 patients with PASC in a post-COVID-19 clinic on demographics, risk factors, illness severity (graded as one-mild to five-severe), functional status, and 29 symptoms and principal component symptoms cluster analysis. The Institute of Medicine (IOM) 2015 criteria were used to determine the Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) phenotype.

Findings

The median age was 47 years, 59.0% were female; 49.3% White, 17.2% Hispanic, 14.9% Asian, and 6.7% Black. Only 12.7% required hospitalization. Seventy-two (53.5%) patients had no known comorbid conditions. Forty-five (33.9%) were significantly debilitated. The median duration of symptoms was 285.5 days, and the number of symptoms was 12. The most common symptoms were fatigue (86.5%), post-exertional malaise (82.8%), brain fog (81.2%), unrefreshing sleep (76.7%), and lethargy (74.6%). Forty-three percent fit the criteria for ME/CFS, majority were female, and obesity (BMI > 30 Kg/m2) (P = 0.00377895) and worse functional status (P = 0.0110474) were significantly associated with ME/CFS.

Interpretations

Most PASC patients evaluated at our clinic had no comorbid condition and were not hospitalized for acute COVID-19. One-third of patients experienced a severe decline in their functional status. About 43% had the ME/CFS subtype.
Liver fibrosis-4 score predicts outcome of patients with ischemic stroke undergoing intravenous thrombolysis
Authors: Norata Davide , Lattanzi Simona , Broggi Serena , Rocchi Chiara , Bartolini Marco , Silvestrini Mauro
Published: February 2023

Some evidence suggests a possible influence of liver disease on stroke prognosis. We investigated the association between fibrosis-4 (FIB-4) score, a marker of liver disease, and the 3-month outcome in patients with ischemic stroke undergoing intravenous thrombolysis. We also evaluated the rate of symptomatic intracranial hemorrhage after thrombolysis. In this prospective cohort study, we enrolled consecutive patients with ischemic stroke treated with thrombolysis who had a 3-month follow-up. The FIB-4 score was calculated and the validated cut-off values were used to indicate high/low risk of advanced liver fibrosis. The primary outcome was 3-month poor prognosis estimated as a modified Rankin scale score ≥3. Of the 264 included patients, 131 (49.62%) had a 3-month mRS ≥3, with a significantly higher FIB-4 score, compared to those with a mRS <3 score (adjp <0.001). When adjusted for possible confounders by multivariate logistic regression, FIB-4 score remained a significant predictor of poor outcome (OR 1.894, p = 0.011), along with history of atrial fibrillation (OR 3.488, p = 0.017), admission NIHSS score (OR 1.305, p < 0.001), and low values of hemoglobin (OR 0.730, p < 0.001). Mechanical thrombectomy had a favorable effect on patients' outcome (OR 0.201, p = 0.005). The risk of poor 3-month outcome was significantly higher among the 32 patients (12.1%) with high risk of severe fibrosis (p = 0.007). FIB-4 score values were also related to symptomatic intracranial hemorrhage (p = 0.004), specifically among patients with high probability of advanced hepatic fibrosis (p = 0.037). FIB-4 score can be considered as a promising independent predictor of poor prognosis in patients with acute ischemic stroke undergoing intravenous thrombolysis.
APRIL: A double-blind, placebo-controlled, randomized, Phase Ib/IIa clinical study of ApTOLL for the treatment of acute ischemic stroke
Authors: Hernández-Jiménez Macarena , Abad-Santos Francisco , Cotgreave Ian , Gallego Jaime , Jilma Bernd , Flores Alan , Jovin Tudor G. , Vivancos José , Molina Carlos A. , Montaner Joan , Casariego Joaquín , Dalsgaard Mads , Hernández-Pérez María , Liebeskind David S. , Cobo Erik , Ribo Marc
Published: February 2023

In the reperfusion era, a new paradigm of treating patients with endovascular treatment (EVT) and neuroprotective drugs is emerging as a promising therapeutic option for patients with acute ischemic stroke (AIS). In this context, ApTOLL, a Toll-like receptor 4 (TLR4) antagonist with proven neuroprotective effect in preclinical models of stroke and a very good pharmacokinetic and safety profile in healthy volunteers, is a promising first-in-class aptamer with the potential to address this huge unmet need. This protocol establishes the clinical trial procedures to conduct a Phase Ib/IIa clinical study (APRIL) to assess ApTOLL tolerability, safety, pharmacokinetics, and biological effect in patients with AIS who are eligible for EVT. This will be a multicenter, double-blind, randomized, placebo-controlled, Phase Ib/IIa clinical study to evaluate the administration of ApTOLL together with EVT in patients with AIS. The study population will be composed of men and non-pregnant women with confirmed AIS with a <6h window from symptoms onset to ApTOLL/placebo administration. The trial is currently being conducted and is divided into two parts: Phase Ib and Phase IIa. In Phase Ib, 32 patients will be allocated to four dose ascending levels to select, based on safety criteria, the best two doses to be administered in the following Phase IIa in which 119 patients will be randomized to three arms of treatment (dose A, dose B, and placebo).

Identification of the trial

EudraCT: 2020-002059-38 and ClinicalTrials.gov Identifier: NCT04734548 https://clinicaltrials.gov/ct2/show/NCT04734548?term=ApTOLL&cond=Stroke&draw=2&rank=1.