bluuebunny/crossref_metadata_2025 huggingface dataset:
Boosting the Sensitivity and Hysteresis of a Gel Polymer Electrolyte by Embedding SiO2 Nanoparticles and PVP for Humidity Applications
Authors: Cedeño Mata Michelle , Orpella Albert , Dominguez-Pumar Manuel , Bermejo Sandra
Published: January 2024

Enhancing sensitivity and hysteresis in capacitance humidity sensors is vital for precise, reliable, and consistent humidity control. This study explores this concern by incorporating polyvinylpyrrolidone (PVP) and SiO2 nanoparticles into a polyvinyl alcohol (PVA)-based ionic liquid gel polymer electrolyte (ILGPE), studying two capacitor types: ILGPE and SiO2 composite ILGPE (CILGPE) capacitors. These novel electrolytes use ammonium acetate as a plasticiser, 1-butyl-3-methylimidazolium bromide as an ionic liquid, SiO2 nanoparticles as a composite, and PVA and PVP as host polymers. Capacitors were characterised and modelled using impedance spectroscopy (IS), providing an electrophysical insight into their working principle. Sensitivity and hysteresis were evaluated within a 20–90% relative humidity (RH) range at 25 °C. The SiO2 CILGPE capacitor with PVP presented superior sensitivity and hysteresis, revealing the beneficial combination of SiO2 nanoparticles and PVP. These benefits are due to the creation of pathways that facilitate water molecule diffusion and crystallinity reduction in PVA-ILGPE. In particular, at 10 kHz, it demonstrates a calibrated capacitance sensitivity of 2660 pF/%RH and a hysteresis of 3.28 %RH. This optimised capacitor outperforms some previous humidity capacitive sensors in sensitivity while exhibiting low hysteresis.
Thermal Properties and Dynamic Rheological Characterization of Dioscorea Starch Gels
Authors: Otegbayo Bolanle Omolara , Tanimola Abiola Rebecca , Ricci Julien , Gibert Olivier
Published: January 2024

Yam (Dioscorea. sp.) is an edible starchy tuber with potential for being a commercial source of starch for industrial purposes, but yam starch is underutilized. The dynamic oscillatory and thermal properties of yam starches from sixteen varieties each of Dioscorea. rotundata, Dioscora. alata, Dioscorea. bulbifera and one variety of Dioscorea. dumetorum from Nigeria were studied to determine their potential for industrial utilization. The storage modulus, loss modulus, damping factor and complex viscosity as a function of frequency (ω) of the dioscorea gels, as well as the onset temperature (To), peak gelatinization temperature (Tp), end of gelatinization (TC), and gelatinization enthalpy of the starches were determined by standard procedures. Results showed that all the dioscorea starches showed a typical elastic behavior with the magnitude of G′ greater than G″ while tan δ < 1 in all varieties. Thus, the starch gels were more elastic than viscous. All the starch gels exhibited shear thinning characteristics and showed frequency (ω) independence characteristics of weak gels. D. rotundata varieties had the lowest ∆Hgel, while D. bulbifera varieties had the highest. The diversity of the visco-elastic and thermal properties of the yam starch gels from different varieties and species can be an advantage in their utilization in both food and non-food industries.
Fabrication of Alginate/Ozoile Gel Microspheres by Electrospray Process
Authors: Ciarleglio Gianluca , Russo Tiziana , Toto Elisa , Santonicola Maria Gabriella
Published: January 2024

Natural polymers, such as alginate and chitosan, are widely exploited for drug delivery applications due to their biocompatibility, low toxicity, and sustainable sourcing. In this study, pH-responsive gel microspheres were fabricated from an alginate/Ozoile emulsion. Ozoile (Stable Ozonides) is a biological inducer, derived from olive oil, which stimulates the endogenous defense system by promoting the repair of tissue damage and restoration of proper physiology through the regulation of gene transcription. Here, the versatile and cost-effective electrospray technique without the use of organic solvents was used to fabricate alginate/Ozoile microspheres with high throughput. The process parameters (voltage, flow rate, and needle gauge) were optimized to obtain microspheres with good sphericity factor and tailored diameter (250–700 μm). The microspheres were additionally optimized through a chitosan coating to enhance their stability and regulate the gel matrix’s degradation process. Morphological analysis, FTIR spectroscopy, and degradation tests confirmed the structural integrity and pH-responsive behavior of the gel microspheres. This research offers a promising route for targeted drug delivery systems, particularly in applications related to the modulation of oxidative stress and management of inflammation.
Parallels in Cartography: Standard, Equidistantly Mapped and True Length Parallels
Authors: Lapaine Miljenko
Published: January 2024

In the literature on map projections, we regularly encounter the name standard parallel or standard parallels. However, it is obvious that a unique definition of a standard parallel is not universally accepted. To fully clarify the meaning of standard parallels, the author proposes the notion of equidistantly mapped parallels, which has not been common in the literature so far. Equidistantly mapped parallels can be in the direction of the parallel or in the direction of the meridian. Here, it is shown that every standard parallel is also an equidistantly mapped parallel, but that the reverse need not be true. If the parallel is mapped equidistantly in the direction of the parallel, then its length in the projection plane is equal to the length of that parallel on the sphere. The opposite does not have to be true, i.e., if the length of the image of the parallel in the projection plane is equal to the length of the parallel on the sphere, this does not mean that the parallel was mapped equidistantly. In addition to standard and equidistant parallels, the concept of parallels of true length also appears in the theory of map projections. They should also be distinguished from standard and equidistant parallels.
Statistics on Typhoon Intensity and Rice Damage in Vietnam and the Philippines
Authors: Yuen Kai Wan , Switzer Adam D. , Teng Paul P. S. , Lee Janice Ser Huay
Published: January 2024

Typhoons are destructive multi-hazard events. To assess the relationship between typhoon intensity and agricultural loss, there is a need for accurate and standardized information on loss and damage, which is currently lacking. To address this, a database for Vietnam and the Philippines was created to provide aggregated information on the magnitude of rice damage and to highlight the rice-growing areas which were prone to being damaged by typhoons. Our study period was from 1970 to 2018, and we focused on Vietnam and the Philippines as these two countries experience frequent and intense typhoons. As different crops respond differently to wind and rain, we limit our research to a single crop. In this study, we focused on rice as it is a major staple food in Southeast Asia, and rice fields were often damaged by typhoons in the two countries. Of the 829 typhoon events recorded, only 15% of the events resulted in rice damage. The average area of rice damaged per typhoon event ranged from 42,407 ha in Vietnam to 83,571 ha in the Philippines. Meanwhile, the average production loss per typhoon event ranged from 190,227 metric tonnes in the Philippines to 539,150 metric tonnes in Vietnam. The monetary value of rice crops lost was only reported in the Philippines, and this amounted to an average of US$ 42 million per typhoon event. There was a weak relationship between landfall wind speed and the three indicators of rice damage, which suggests that rice damage was not primarily due to strong winds. Our results showed that the rice fields in the coastal provinces of Vietnam and the northern parts of the Philippines were more vulnerable to being damaged by typhoons.
The Lac Fallère Area as an Example of the Interplay between Deep-Seated Gravitational Slope Deformation and Glacial Shaping (Aosta Valley, NW Italy)
Authors: Dolce Stefano , Forno Maria Gabriella , Gattiglio Marco , Gianotti Franco
Published: January 2024

The Lac Fallère area in the upper Clusellaz Valley (tributary of the middle Aosta Valley) is shaped in micaschist and gneiss (Mont Fort Unit, Middle Penninic) and in calcschist and marble (Aouilletta Unit, Combin Zone). Lac Fallère exhibits an elongated shape and is hosted in a WSW–ENE-trending depression, according to the slope direction. This lake also shows a semi-submerged WSW–ENE rocky ridge that longitudinally divides the lake. This evidence, in addition to the extremely fractured rocks, indicates a wide, deep-seated gravitational slope deformation (DSGSD), even if this area is not yet included within the regional landslide inventory of the Aosta Valley Region. The Lac Fallère area also shows reliefs involved in glacial erosion (roches moutonnée), an extensive cover of subglacial sediments, and many moraines essentially referred to as Lateglacial. The DSGSD evolution in a glacial environment produced, as observed in other areas, effects on the facies of Quaternary sediments and the formation of a lot of wide moraines. Glacial slope sectors and lateral moraines displaced by minor scarps and counterscarps, and glaciers using trenches forming several arched moraines, suggest an interplay between glacial and gravitational processes, which share part of their evolution history.
The Influence of Physical, Mental, and Cognitive Factors on Health-Related Quality of Life among Community-Dwelling Older Adults: A Focus on Central Sensitization-Related Symptoms
Authors: Kikuchi Yuki , Nakano Hideki , Goda Akio , Mori Kohei , Abiko Teppei , Mitsumaru Nozomi , Murata Shin
Published: January 2024

Most older adults wish to maintain independence in their familiar communities. However, many experience pain and pain-related disabilities which reduce their health-related quality of life (HRQOL), leading to increased hospitalizations and mortality. This study aimed to determine the impact of physical, mental, and cognitive factors, particularly central sensitization-related symptoms (CSS), on the HRQOL of community-dwelling older adults. A total of 206 participants were included in the analysis, which measured HRQOL, basic attributes, physical functions and body pain, mental factors, cognitive factors, and CSS severity using validated tools. A correlation analysis was used to examine the association between HRQOL and each measure. Furthermore, multiple regression analysis (forced entry method) was performed to identify the factors influencing the HRQOL. The study found that pain intensity and CSS severity significantly influenced the HRQOL among community-dwelling older adults. The higher the pain intensity and CSS severity, the lower their HRQOL. The participants had mild pain and CSS, demonstrating the need to monitor, address, and treat even non-severe issues in community-dwelling older adults. This association, revealed for the first time in this study, suggests that approaches to reduce pain and CSS are important for maintaining and improving the HRQOL of community-dwelling older adults.
Sexual Rewards and Costs in Heterosexual and Gay Hispanic Adults
Authors: Calvillo Cristobal , Sierra Juan Carlos , Sánchez-Fuentes María del Mar , Granados Reina
Published: January 2024

The Interpersonal Exchange Model of Sexual Satisfaction proposes that sexual satisfaction can be explained by the balance of sexual rewards or costs exchange. The Interpersonal Exchange Model of Sexual Satisfaction Questionnaire (IEMSSQ) was developed from this model. The IEMSSQ includes the Reward/Costs Checklist (RCC). The RCC assesses different sexual exchanges that are categorized into sexual rewards and/or costs. Analyses and comparisons of sexual rewards and costs in the Hispanic cisgender population based on gender (man or woman) and sexual orientation (heterosexual and homosexual) with this measure have not been conducted. The main goal was to analyze and rank the sexual rewards, costs, and both, indicated by a sample of 1996 Hispanic partnered participants (heterosexual men, gay men, heterosexual women, and lesbians). The predominant sexual exchanges that were reported involved emotional aspects, both as rewards and costs. When participants indicated that the exchange was both a reward and a cost, they were referring to aspects specifically related to the sexual relationship itself. Heterosexual men and women had greater rewards and higher costs, respectively. Gay men obtained a greater number of items reported as rewards and costs. The results provide further insight into sexual satisfaction related to gender and sexual orientation.
Interventions for Compassion Fatigue in Healthcare Providers—A Systematic Review of Randomised Controlled Trials
Authors: Patole Sanjay , Pawale Dinesh , Rath Chandra
Published: January 2024

Background: Compassion fatigue is a significant issue considering its consequences including negative feelings, impaired cognition, and increased risk of long-term morbidities. We aimed to assess current evidence on the effects of interventions for compassion fatigue in healthcare providers (HCP). Methods: We used the Cochrane methodology for Systematic Reviews and Meta-Analyses (PRISMA) for conducting and reporting this review. Results: Fifteen RCTs (n = 1740) were included. The sample size of individual studies was small ranging from 23 to 605. There was significant heterogeneity in participant, intervention, control, and outcome characteristics. The tools for assessing intervention effects on compassion fatigue included ProQOL, compassion fatigue scale, and nurses compassion fatigue inventory. Thirteen out of the fifteen included RCTs had overall high risk of bias (ROB). Meta-analysis could not be performed given the significant heterogeneity. Conclusions: Current evidence on interventions for reducing compassion fatigue in HCPs is inadequate. Given the benefits reported in some of the included studies, well-designed and adequately powered RCTs are urgently needed.
Nurses’ Well-Being at Work in a Hospital Setting: A Scoping Review
Authors: Almeida Débora , Figueiredo Ana Rita , Lucas Pedro
Published: January 2024

The aim of this review was to analyze the scientific evidence about nurses’ well-being at work in the hospital context. Well-being is present in our daily experiences, whether in a personal or professional context. Nurses are frequently put under pressure and stressed at work, which can influence their well-being. Nurses’ well-being at work in a hospital setting is crucial due to its relevance to occupational health, the quality of patient care, and the identification of stress and satisfaction factors. Methods: This systematic review followed the methodological guidelines of the Joanna Briggs Institute (JBI). The databases searched included CINAHL, MEDLINE, Scopus, Cochrane Database of Systematic Reviews, LILACS, Scientific Electronic Library Online (SciELO), and the Open Access Scientific Repositories of Portugal (RCAAP). The following inclusion criteria were defined: studies in Portuguese or English; with abstracts or full texts available; with a publication date from 2018 to 2022; and research containing the identified keywords in the title (TI) or abstract (AB). To organize and synthesize the data, we used a table to extract the significant information from each included study. Results: Eight studies were included, all of them emphasizing the assessment of well-being at work and the manager’s intervention to promote this well-being. We found that most studies indicate that nurses are satisfied with their work. There are several factors that can influence this positive level of well-being at work, such as interaction with management, culture, and organizational commitment. It can be concluded that nurse managers have a decisive role in promoting well-being at work.
Healthcare Social Workers’ Scope of Practice during COVID-19
Authors: Washington Tiffany , Lewinson Terri D.
Published: January 2024

The COVID-19 pandemic pushed the U.S. healthcare system to its limits, resulting in the need for flexibility in care delivery. This study aimed to describe healthcare social workers’ scope of practice since the start of the pandemic. Semi-structured interviews for this qualitative study were conducted using the Zoom platform between July and August 2020. This study used a basic qualitative content analysis with integrated deductive and inductive coding to explore participant perspectives. Their scope was assessed based on healthcare social work practice standards. Four practice standards and eight themes that emerged from the data were knowledge and skills (care planning and intervention and social worker–patient relationship), workload sustainability (workload expansion and workload facilitators), interdisciplinary collaboration (collaborating beyond the scope of responsibilities and collaboration challenges), and cultural competency (institutional and societal). The findings add a deeper understanding of the roles social workers perform, how they think about these roles, how they want to be understood, and how they are best utilized in ways consistent with their training and expertise. Moving forward, healthcare systems may consider well-delineated roles and responsibilities for everyday practice and during pandemics.
Occupational Risk Factors for Musculoskeletal Disorders among Workers in Dairy Diversification
Authors: Buisseret Fabien , Draye Nicolas , Di Santo Camille , Pacewicz Jocelyn , Pannetier Johanna , Dierick Frédéric , Telliez Frédéric
Published: January 2024

Background: In a changing European agricultural context, diversification of dairy farms is gaining attention. This study seeks to (1) assess musculoskeletal pain prevalence associated with tasks such as butter, yogurt, and cheese production; and (2) analyze associated risks. Methods: Observing 31, mostly female, workers, we utilized the ERGOROM questionnaire, a methodology adapted from the Institut National de Recherche et de Sécurité, and Key Indicator Method forms. Results: Findings revealed that tasks like load carrying (42% of workers), manual work (17%), and awkward postures (14%) resulted in musculoskeletal pain, predominantly in the lower back (65%), neck (39%), and dominant upper limb areas (shoulder: 61%, elbow: 26%, and wrist: 65%). While psychosocial risks remained low, concerns arose from workload, hygiene standards, and resource unpredictability. Conclusions: As dairy farming evolves from artisanal to semi-industrial, our study emphasizes the importance of ergonomic adaptations to protect farmers’ health and prevent musculoskeletal disorders during diversification.
Correction: Mohammed et al. Assessment of Knowledge, Attitude, and Practice towards Tuberculosis among Taif University Students. Healthcare 2023, 11, 2807
Authors: Mohammed Eilaf A. , Alotaibi Huriyyah A. , Alnemari Joud F. , Althobiti Meznah S. , Alotaibi Shumukh S. , Ewis Ashraf A. , El-Sheikh Azza A. K. , Abdelwahab Sayed F.
Published: January 2024

In the original publication [...]
Study on Italian Onion Cultivars/Ecotypes towards Onion Yellow Dwarf Virus (OYDV) Infection
Authors: Corrado Carla Libia , Micali Giuseppe , Mauceri Antonio , Bertin Sabrina , Sunseri Francesco , Abenavoli Maria Rosa , Tiberini Antonio
Published: January 2024

The onion yellow dwarf virus (OYDV) represents a limiting biotic stress in onion (Allium cepa L.); little information is available regarding resistant varieties. In Italy, onion production is limited but represented by a wide diversity of ecotypes. A two-year trial was carried out to test the OYDV-susceptibility/tolerance of different Italian onion cultivars by rating symptom severity and plant growth parameters and assessing post-harvest secondary infections. The cultivar and ecotypes included in the study were characterized by simple sequence repeats (SSR) analysis, and the expression analysis of two genes (Eukaryotic translation initiation factors, EIFs) involved in potyvirus replication was also performed. Two susceptible and one tolerant cultivar were identified based on symptom expression and virus impact on plants. Although differences in growth parameters were limited to the first-year trial, the infection was correlated to a higher incidence of secondary infections in post-harvest, with altered water balance in infected bulbs. This correlation was also demonstrated during the long-term storage of bulbs. SSR analysis identified different clusters and only one gene isoform (EIF4eiso1) showed different expression levels in the OYDV/onion pathosystem. In conclusion, this study defines the genetic profile of Italian onion cultivars and provides evidence on susceptibility/tolerance features which will be useful in the future for the identification of viral resistance traits in onion.
Using Machine Learning to Improve Vector Control, Public Health and Reduce Fragmentation of Urban Water Management
Authors: Klafke Fernanda , Henning Elisa , Barros Virginia Grace
Published: January 2024

Urban waters (UW) are complex environments, and their definition is related to water systems in urban zones, whether in a natural system or an urban facility. The health of these environments is related to public health and the quality of life because public health is the focal point of environmental and anthropic impacts. Infrastructure is paramount for maintaining public health and social and economic development sanitation. Insufficient infrastructure favors disease vectors. The population and environment suffer from deficient urban water infrastructure in Brazil despite government efforts to manage the existing systems. In this work, machine learning (regression trees) demonstrates the deficiency of sanitation and UW management fragmentation on public health by using the Aedes aegypti infestation index (HI) and water supply, wastewater, stormwater and drainage indicators (SNIS data). The results show that each Brazilian region faces different problems. The more infested regions were Northeastern, Northern and Southeastern. Moreover, municipalities with better SNIS data have lower infestation rates. Minimizing problems related to sanitation through the integrated management of water and urban areas is extremely important in developing countries. UW governance is connected to public health. Water management fragmentation leads to more complex issues, and managers must confront them to improve the quality of life in urban zones.
Semantic-Enhanced Graph Convolutional Neural Networks for Multi-Scale Urban Functional-Feature Identification Based on Human Mobility
Authors: Chen Yuting , Zhao Pengjun , Lin Yi , Sun Yushi , Chen Rui , Yu Ling , Liu Yu
Published: January 2024

Precise identification of spatial unit functional features in the city is a pre-condition for urban planning and policy-making. However, inferring unknown attributes of urban spatial units from data mining of spatial interaction remains a challenge in geographic information science. Although neural-network approaches have been widely applied to this field, urban dynamics, spatial semantics, and their relationship with urban functional features have not been deeply discussed. To this end, we proposed semantic-enhanced graph convolutional neural networks (GCNNs) to facilitate the multi-scale embedding of urban spatial units, based on which the identification of urban land use is achieved by leveraging the characteristics of human mobility extracted from the largest mobile phone datasets to date. Given the heterogeneity of multi-modal spatial data, we introduced the combination of a systematic data-alignment method and a generative feature-fusion method for the robust construction of heterogeneous graphs, providing an adaptive solution to improve GCNNs’ performance in node-classification tasks. Our work explicitly examined the scale effect on GCNN backbones, for the first time. The results prove that large-scale tasks are more sensitive to the directionality of spatial interaction, and small-scale tasks are more sensitive to the adjacency of spatial interaction. Quantitative experiments conducted in Shenzhen demonstrate the superior performance of our proposed framework compared to state-of-the-art methods. The best accuracy is achieved by the inductive GraphSAGE model at the scale of 250 m, exceeding the baseline by 25.4%. Furthermore, we innovatively explained the role of spatial-interaction factors in the identification of urban land use through the deep learning method.
The Binding of Pseudomonas aeruginosa to Cystic Fibrosis Bronchial Epithelial Model Cells Alters the Composition of the Exosomes They Produce Compared to Healthy Control Cells
Authors: Lozano-Iturbe Víctor , Blanco-Agudín Noelia , Vázquez-Espinosa Emma , Fernández-Vega Iván , Merayo-Lloves Jesús , Vazquez Fernando , Girón Rosa M. , Quirós Luis M.
Published: January 2024

Cystic fibrosis (CF) is a genetic disease that causes dehydration of the surface of the airways, increasing lung infections, most frequently caused by Pseudomonas aeruginosa. Exosomes are nanovesicles released by cells that play an essential role in intercellular communication, although their role during bacterial infections is not well understood. In this article, we analyze the alterations in exosomes produced by healthy bronchial epithelial and cystic fibrosis cell lines caused by the interaction with P. aeruginosa. The proteomic study detected alterations in 30% of the species analyzed. In healthy cells, they mainly involve proteins related to the extracellular matrix, cytoskeleton, and various catabolic enzymes. In CF, proteins related to the cytoskeleton and matrix, in addition to the proteasome. These differences could be related to the inflammatory response. A study of miRNAs detected alterations in 18% of the species analyzed. The prediction of their potential biological targets identified 7149 genes, regulated by up to 7 different miRNAs. The identification of their functions showed that they preferentially affected molecules involved in binding and catalytic activities, although with differences between cell types. In conclusion, this study shows differences in exosomes between CF and healthy cells that could be involved in the response to infection.
Temperature-Dependent Olive Pomace Extraction for Obtaining Bioactive Compounds Preventing the Death of Murine Cortical Neurons
Authors: Casazza Alessandro Alberto , Capraro Michela , Pedrazzi Marco , D’Agostino Giulia , Onofri Franco , Marte Antonella , De Tullio Roberta , Perego Patrizia , Averna Monica
Published: January 2024

High-pressure and temperature extraction (HPTE) can effectively recover bioactive compounds from olive pomace (OP). HPTE extract obtained by extracting OP with ethanol and water (50:50 v/v) at 180 °C for 90 min demonstrated a pronounced ability to preserve intracellular calcium homeostasis, shielding neurons from the harmful effects induced by N-methyl-d-aspartate (NMDA) receptor (NMDAR) overactivation, such as aberrant calpain activation. In this study, the extraction temperature was changed from 37 to 180 °C, and the extracts were evaluated for their antioxidant potency and ability to preserve crucial intracellular Ca2+-homeostasis necessary for neuronal survival. Additionally, to verify the temperature-induced activity of the extract, further extractions on the exhausted olive pomace were conducted, aiming to identify variations in the quality and quantity of extracted phenolic molecules through HPLC analysis. The results revealed a significant increase in bioactive compounds as a function of temperature variation, reaching 6.31 ± 0.09 mgCAE/mL extract for the extraction performed at 180 °C. Subsequent extraction of the exhausted residues yielded extracts that remained active in preventing calcium-induced cell death. Moreover, despite increased antiradical power, extracts re-treated at 180 °C did not display cell protection activity. Our results indicate that the molecules able to maintain physiological Ca2+-homeostasis in murine cortical neurons in conditions of cytotoxic stimulation of NMDAR are wholly recovered from olive pomace only following extraction performed at 180 °C.
Carvacrol as a Stimulant of the Expression of Key Genes of the Ginsenoside Biosynthesis Pathway and Its Effect on the Production of Ginseng Saponins in Panax quinquefolium Hairy Root Cultures
Authors: Kochan Ewa , Sienkiewicz Monika , Szmajda-Krygier Dagmara , Balcerczak Ewa , Szymańska Grażyna
Published: January 2024

The accumulation of ginsenosides (triterpenic saponins) was determined in Panax quinquefolium hairy root cultures subjected to an elicitation process using carvacrol at 5, 10, 25, 50, 100, 250, and 500 μM concentrations during 24 and 72 h exposure. This study was the first one in which carvacrol was applied as an elicitor. The content of eight ginsenosides, Rb1, Rb2, Rb3, Rc, Rd, Rg1, Rg2, and Re, was determined using HPLC analysis. Moreover, the quantitative RT-PCR method was applied to assess the relative expression level of farnesyl diphosphate synthase, squalene synthase, and dammarenediol synthase genes in the studied cultures. The addition of carvacrol (100 μM) was an effective approach to increase the production of ginsenosides. The highest content and productivity of all detected saponins were, respectively, 20.01 mg∙g−1 d.w. and 5.74 mg∙L−1∙day−1 after 72 h elicitation. The production profile of individual metabolites in P. quinquefolium cultures changed under the influence of carvacrol. The biosynthesis of most examined protopanaxadiol derivatives was reduced under carvacrol treatment. In contrast, the levels of ginsenosides belonging to the Rg group increased. The strongest effect of carvacrol was noticed for Re metabolites, achieving a 7.72-fold increase in comparison to the control. Saponin Rg2, not detected in untreated samples, was accumulated after carvacrol stimulation, reaching its maximum concentration after 72 h exposure to 10 μM elicitor.
Cortical and Striatal Astrocytes of Neonatal Rats Display Distinct Molecular and Pharmacological Characteristics of Dopamine Uptake
Authors: Sočan Vesna , Dolinar Klemen , Kržan Mojca
Published: January 2024

Astrocytes are crucial in the regulation of neurotransmitter homeostasis, and while their involvement in the dopamine (DA) tripartite synapse is acknowledged, it necessitates a more comprehensive investigation. In the present study, experiments were conducted on primary astrocyte cultures from the striatum and cortex of neonatal rats. The pharmacological intricacies of DA uptake, including dependence on time, temperature, and concentration, were investigated using radiolabelled [3H]-DA. The mRNA expression of transporters DAT, NET, PMAT, and OCTs was evaluated by qPCR. Notably, astrocytes from both brain regions exhibited prominent mRNA expression of NET and PMAT, with comparatively lower expression of DAT and OCTs. The inhibition of DA uptake by the DAT inhibitor, GBR12909, and NET inhibitors, desipramine and nortriptyline, impeded DA uptake in striatal astrocytes more than in cortical astrocytes. The mRNA expression of NET and PMAT was significantly upregulated in cortical astrocytes in response to the DA receptor agonist apomorphine, while only the mRNA expression of NET exhibited changes in striatal astrocytes. Haloperidol, a DA receptor antagonist, and L-DOPA, a DA precursor, did not induce significant alterations in transporter mRNA expression. These findings underscore the intricate and region-specific mechanisms governing DA uptake in astrocytes, emphasizing the need for continued exploration to unravel the nuanced dynamics of astrocytic involvement in the DA tripartite synapse.
The Inhibition of Vessel Co-Option as an Emerging Strategy for Cancer Therapy
Authors: Carrera-Aguado Iván , Marcos-Zazo Laura , Carrancio-Salán Patricia , Guerra-Paes Elena , Sánchez-Juanes Fernando , Muñoz-Félix José M.
Published: January 2024

Vessel co-option (VCO) is a non-angiogenic mechanism of vascularization that has been associated to anti-angiogenic therapy. In VCO, cancer cells hijack the pre-existing blood vessels and use them to obtain oxygen and nutrients and invade adjacent tissue. Multiple primary tumors and metastases undergo VCO in highly vascularized tissues such as the lungs, liver or brain. VCO has been associated with a worse prognosis. The cellular and molecular mechanisms that undergo VCO are poorly understood. Recent studies have demonstrated that co-opted vessels show a quiescent phenotype in contrast to angiogenic tumor blood vessels. On the other hand, it is believed that during VCO, cancer cells are adhered to basement membrane from pre-existing blood vessels by using integrins, show enhanced motility and a mesenchymal phenotype. Other components of the tumor microenvironment (TME) such as extracellular matrix, immune cells or extracellular vesicles play important roles in vessel co-option maintenance. There are no strategies to inhibit VCO, and thus, to eliminate resistance to anti-angiogenic therapy. This review summarizes all the molecular mechanisms involved in vessel co-option analyzing the possible therapeutic strategies to inhibit this process.
Calcium Electroporation versus Electrochemotherapy with Bleomycin in an In Vivo CAM-Based Uveal Melanoma Xenograft Model
Authors: Tsimpaki Theodora , Anastasova Ralitsa , Liu Hongtao , Seitz Berthold , Bechrakis Nikolaos E. , Berchner-Pfannschmidt Utta , Kraemer Miriam M. , Fiorentzis Miltiadis
Published: January 2024

Despite recent advancements in the diagnosis and treatment of uveal melanoma (UM), its metastatic rate remains high and is accompanied by a highly dismal prognosis, constituting an unmet need for the development of novel adjuvant therapeutic strategies. We established an in vivo chick chorioallantoic membrane (CAM)-based UM xenograft model from UPMD2 and UPMM3 cell lines to examine its feasibility for the improvement of selection of drug candidates. The efficacy of calcium electroporation (CaEP) with 5 or 10 mM calcium chloride (Ca) and electrochemotherapy (ECT) with 1 or 2.5 µg/mL bleomycin in comparison to monotherapy with the tested drug or electroporation (EP) alone was investigated on the generated UM tumors. CaEP and ECT showed a similar reduction of proliferation and melanocytic expansion with a dose-dependent effect for bleomycin, whereas CaEP induced a significant increase of the apoptosis and a reduction of vascularization with varying sensitivity for the two xenograft types. Our in vivo results suggest that CaEP and ECT may facilitate the adequate local tumor control and contribute to the preservation of the bulbus, potentially opening new horizons in the adjuvant treatment of advanced UM.
Special Issue “Adipokines, Myokines, and Physical Exercise in Health and Disease 2.0”
Authors: Bilski Jan , Brzozowski Tomasz
Published: January 2024

We are pleased to present our Editorial to this Special Issue on “Adipokines, Myokines, and Physical Exercise in Health and Disease 2 [...]
Scheduling of Remote Monitoring for Peritoneal Dialysis Patients
Authors: Virzì Grazia Maria , Morisi Niccolò , Milan Manani Sabrina , Tantillo Ilaria , Gonzàlez Barajas José David , Villavicencio Bladimir Diaz , Castiglione Claudia , Alfano Gaetano , Donati Gabriele , Zanella Monica
Published: January 2024

Peritoneal dialysis (PD) is performed as a home-based treatment and in this context, telemedicine has been proven helpful for improving clinicians’ surveillance and maintaining PD patients in their home setting. The new e-health devices make remote patient monitoring (RPM) for automated peritoneal dialysis (APD) treatment possible, evaluating the data at the end of every treatment and adapting the prescription at distance if necessary. This paper aims to share a method for improving clinical surveillance and enabling PD patients to receive their treatment at home. In the present case series, we delineate the clinical protocol of the Vicenza PD Center regarding patient characteristics, timing, and the purpose of the APD-RPM. We present the Vicenza PD Center’s experience, illustrating its application through three case reports as exemplars. Telemedicine helps to carefully allocate healthcare resources while removing the barriers to accessing care. However, there is a risk of data overload, as some data might not be analyzed because of an increased workload for healthcare professionals. A proactive physician’s attitude towards the e-health system has to be supported by clinical instructions and legislative rules. International and national guidelines may suggest which patients should be candidates for RPM, which parameters should be monitored, and with what timing. According to our experience, we suggest that the care team should define a workflow that helps in formulating a correct approach to RPM, adequately utilizing resources. The workflow has to consider the different needs of patients, in order to assure frequent remote control for incident or unstable patients, while prevalent and stable patients can perform their home treatment more independently, helped by periodic and deferred clinical supervision.
Harnessing Non-Thermal Plasma to Supercharge Recovery in Abdominal Surgeries: A Pilot Study
Authors: Rodríguez-Méndez Benjamín G. , López-Callejas Régulo , Mercado-Cabrera Antonio , Peña-Eguiluz Rosendo , Valencia-Alvarado Raúl , Betancourt-Ángeles Mario , Berrones-Stringel Guillermo , Jaramillo-Martínez César
Published: January 2024

(1) Background: This study aims to evaluate the efficacy and safety of non-thermal plasma (NTP) therapy in accelerating wound healing in patients who have undergone laparoscopic and open surgeries. (2) Methods: NTP was applied using a needle-type reactor with an irradiance of 0.5 W/cm2 on the surgical wounds of fifty patients after obtaining informed consent. Three NTP treatments, each lasting three minutes, were administered hourly. (3) Results: The pilot study showed that NTP-treated surgical wounds healed completely without any signs of infection, dehiscence, pain, or itching. Notably, patients reported minimal pain after the NTP treatment. Visual assessments conducted twenty-four hours after surgery revealed no redness or fluid discharge. Comparisons with traditionally sutured wounds indicated that NTP-treated wounds healed at a rate equivalent to seven days. (4) Conclusions: The application of NTP in laparoscopic and open wounds proved safe and effective, expediting the wound healing process and eliminating clinical risks post-surgery. Significantly, NTP facilitated a healing rate within twenty-four hours, equivalent to seven days for suture-treated wounds, significantly reducing the hospitalization time to a single day. These findings highlight the potential of NTP to be a transformative approach for promoting postoperative recovery.
The Effectiveness of Cognitive Behavioral Therapy on Depression and Sleep Problems for Climacteric Women: A Systematic Review and Meta-Analysis
Authors: Kim Ji-Hyun , Yu Hea-Jin
Published: January 2024

(1) Background: Women in their middle years undergoing perimenopause encounter a range of physical and psychological alterations attributed to hormonal changes. The prominent symptoms among menopausal women are depressive symptoms and sleep-related problems. The aim of this study was to conduct a meta-analysis examining the effects of Cognitive Behavioral Therapy (CBT) on women going through menopause, specifically focusing on depressive symptoms and sleep problems. We analyzed studies conducted both within the country and across international settings over the last decade. (2) Methods: A search of the literature was conducted—a targeted search, exclusively considering randomized controlled trials (RCTs) that were published within the timeframe spanning from 15 June 2013 to 15 June 2023. (3) Findings: Upon reviewing nine studies that satisfied our inclusion criteria and involved a total of 923 participants, it was noted that four of these studies incorporated diverse cognitive-behavioral strategies. Among the nine studies, a total of four were included in the meta-analysis: two measured depressive symptoms, and two measured sleep quality. The combined effect size for depressive symptoms was found to be 3.55 (95% confidence interval: −5.48, −1.61; p < 0.05), and for sleep quality, it was 0.78 (95% confidence interval: −1.32, −0.25; p = 0.004). (4) Conclusions: Our review emphasizes the necessity for conducting larger-scale studies focused on the application of CBT for women experiencing menopausal symptoms. Additionally, it is recommended to approach the interpretation of these results with caution due to discrepancies in methodology and the overall quality of the studies. Further clinical trials are necessary to establish the ideal number of CBT sessions needed for the effective treatment of depression in menopausal women. Future studies should cover a wider range of geographical locations, including more countries, and focus on various outcomes such as depressive symptoms and sleep quality.
Recent Advances in pH and Redox Responsive Polymer Nanocomposites for Cancer Therapy
Authors: Gaddimath Shivalingayya , Payamalle Shivanand , Channabasavana Hundi Puttaningaiah Keshavananada Prabhu , Hur Jaehyun
Published: January 2024

Cancer therapy currently focuses on personalized targeted treatments. A promising approach uses stimuli-responsive biomaterials for site-specific drug release, such as pH- and redox-triggered polymer nanocomposites. These materials respond to the tumor microenvironment, enhance efficacy, and reduce off-target effects. Cancer cells with anomalous properties such as acidic cytosolic pH and elevated redox potential are targeted by these biomaterials. An imbalance in ions and biological thiols in the cytoplasm contributes to tumor growth. Functionalized polymer nanocomposites with large surface areas and specific targeting outperform conventional small-molecule materials. To overcome problems such as low bioavailability, uncontrolled drug release, and poor cell penetration, multifunctional nanomaterials make it easier for drugs to enter certain cellular or subcellular systems. High therapeutic efficacy is achieved through surface functionalization, site-specific targeting, and the use of stimuli-responsive components. In particular, pH and redox dual-stimuli-based polymeric nanocomposites for cancer therapeutics have scarcely been reported. This article provides recent progress in pH- and redox-responsive polymer nanocomposites for site-specific drug delivery in cancer therapy. It explores the design principles, fabrication methods, mechanisms of action, and prospects of these dual-stimuli-responsive biomaterials.
ControlFace: Feature Disentangling for Controllable Face Swapping
Authors: Zhang Xuehai , Zhou Wenbo , Liu Kunlin , Tang Hao , Zhang Zhenyu , Zhang Weiming , Yu Nenghai
Published: January 2024

Face swapping is an intriguing and intricate task in the field of computer vision. Currently, most mainstream face swapping methods employ face recognition models to extract identity features and inject them into the generation process. Nonetheless, such methods often struggle to effectively transfer identity information, which leads to generated results failing to achieve a high identity similarity to the source face. Furthermore, if we can accurately disentangle identity information, we can achieve controllable face swapping, thereby providing more choices to users. In pursuit of this goal, we propose a new face swapping framework (ControlFace) based on the disentanglement of identity information. We disentangle the structure and texture of the source face, encoding and characterizing them in the form of feature embeddings separately. According to the semantic level of each feature representation, we inject them into the corresponding feature mapper and fuse them adequately in the latent space of StyleGAN. Owing to such disentanglement of structure and texture, we are able to controllably transfer parts of the identity features. Extensive experiments and comparisons with state-of-the-art face swapping methods demonstrate the superiority of our face swapping framework in terms of transferring identity information, producing high-quality face images, and controllable face swapping.
Development of a Numerical Prediction Model for Marine Lower Atmospheric Ducts and Its Evaluation across the South China Sea
Authors: Liu Qian , Zhao Xiaofeng , Zou Jing , Li Yunzhou , Qiu Zhijin , Hu Tong , Wang Bo , Li Zhiqian
Published: January 2024

The Coupled Ocean–Atmosphere–Wave–Sediment Transport (COAWST) model serves as the foundation for creating a forecast model to detect lower atmospheric ducts in this study. A set of prediction tests with different forecasting times focusing on the South China Sea domain was conducted to evaluate the short-term forecasting effectiveness of lower atmospheric ducts. The assessment of sounding observation data revealed that the prediction model performed well in predicting the characteristics of all types of ducts. The mean values of the forecasting errors were slightly lower than the reanalysis data but had lower levels of correlation coefficients. At an altitude of about 2000 m, the forecasted error of modified atmospheric refractivity reached peak values and then decreased gradually with increasing altitude. The accuracy of forecasted surface ducts was higher than that of elevated ducts. Noticeable land–sea differences were identified for the spatial distributions of duct characteristics, and the occurrence rates of both the surface and elevated ducts were high at sea. As for the differences among the forecasts of 24, 48, and 72 h ahead, the differences primarily occurred at altitude levels below 20 m and 500 m~1500 m, which are consistent with the differences in the duct height.
Identification of Multi-Innovation Stochastic Gradients with Maximum Likelihood Algorithm Based on Ship Maneuverability and Wave Peak Models
Authors: Liu Yang , Zhang Qiang , Wang Longjin , An Shun , He Yan , Fan Zhimin , Deng Fang
Published: January 2024

This paper investigates the problem of real-time parameter identification for ship maneuvering parameters and wave peak frequency in an ocean environment. Based on the idea of Euler discretion, a combined model of ship maneuvering and wave peak frequency (ship–wave) is made a discretion, and a discrete-time auto-regressive moving-average model with exogenous input (ARMAX) is derived for parameter identification. Based on the ideas of stochastic gradient identification and multi-innovation theory, a multi-innovation stochastic gradient (MI-SG) algorithm is derived for parameter identification of the ship–wave discretion model. Maximum likelihood theory is introduced to propose a maximum likelihood-based multi-innovation stochastic gradient (ML-MI-SG) algorithm. Compared to the MI-SG algorithm, the ML-MI-SG algorithm shows improvements in both parameter identification accuracy and identification convergence speed. Simulation results verify the effectiveness of the proposed algorithm.