bluuebunny/crossref_metadata_2025 huggingface dataset:
TABLA DE CONTENIDO LUX MÉDICA 31
Authors: Terrones-Saldívar Ma del Carmen
Published: March 2021

Presenta información general de la revista y su contenido.
ARS MÉDICA
Authors: López-y de la peña Xavier A.
Published: March 2021

Espacio dedicado a escritores y artistas, miembros o no, de la comunidad médica, quienes podrán aportar textos y obras artísticas que contribuyan a mejorar la cultura en salud de la comunidad.Publicación financiada con recursos PFCE 2017.
Anestesia libre de opioides: revisión y reporte de un caso
Authors: Pérez-Atilano Tania Alexis , Olvera-Romo Javier
Published: March 2021

La anestesia libre de opioides es una excelente opción para pacientes que serán sometidos a cirugía ambulatoria ya que ofrece una adecuada analgesia quirúrgica y postoperatoria sin los efectos secundarios de los opioides, lo que se traduce en una menor estancia intrahospitalaria. Se presenta un caso de anestesia libre de opioides a base de infusiones de lidocaína, dexmedetomidina y propofol donde demostramos la estabilidad hemodinámica durante la cirugía y el control del dolor posquirúrgico. LUX MÉDICA AÑO 11, NÚMERO 33, MAYO-AGOSTO 2016 PP 29-36.
Correlación entre el nivel de dependencia funcional del adulto mayor y nivel de sobrecarga del cuidador primario en un hospital público de Aguascalientes
Authors: Martínez-García Itzeen Itzamatul Guadalupe , Gallardo-García David , González-Benítez María Fernanda , Guel-Cruz Karla del Rocío , Aguilera-Ibarra Karen Dinorah , González-Flores Silvia Patricia
Published: March 2021

Introducción. Las personas dependientes requieren de cuidados continuos y en ocasiones extenuantes que pueden tener repercusiones de salud en su cuidador primario. Objetivo: Determinar la correlación del nivel de sobrecarga del cuidador primario en relación con el nivel de dependencia del adulto mayor. Metodología: Estudio descriptivo, correlacional, transversal y prospectivo, instrumentos utilizados test de Zarit con confiabilidad de 0.84 en Chrombach y la escala de Katz con una confiabilidad de 0.98 en alfa de Crombach, para el análisis de datos se utilizó el paquete estadístico SPSS 23 ®, usando la prueba de correlación estadística de Spearman. Resultados: Muestra de 70 adultos mayores hospitalizados en un hospital de segundo nivel en el estado de Aguascalientes; edad predominante entre 60 y 69 años, predominando el sexo masculino; se obtuvo que a mayor nivel de dependencia del adulto mayor no existe un aumento del nivel de sobrecarga del cuidador primario, mediante un coeficiente de correlación rho de Spearman de 0.070 p<0.05. Conclusión: La dependencia funcional del adulto mayor no significa un incremento en el nivel de sobrecarga del adulto mayor, sino que se tiene que valorar su perfil patológico, y tiempo de evolución de las enfermedades.
Asociación entre enfermedad periodontal y la morbimortalidad en pacientes con enfermedad renal crónica en hemodiálisis
Authors: Viana Rojas Jesús Antonio , Gordillo Moscoso Antonio Augusto , López Quijano José Manuel , Pozos Guillén Amaury de Jesús , Rosas Cabral Alejandro
Published: March 2021

Las enfermedades cardiovasculares –como los infartos al miocardio o los accidentes cerebrovasculares– y las enfermedades infecciosas representan las principales causas de morbimortalidad en pacientes con enfermedad renal crónica en hemodiálisis. La enfermedad periodontal es un proceso inflamatorio local de los tejidos de soporte dentario, tales como la encía, el cemento, el ligamento periodontal y el hueso alveolar. La presencia de la enfermedad periodontal en pacientes con enfermedad renal crónica terminal (ERCT) está favorecida por múltiples mecanismos patogénicos. En este artículo se revisa la posible asociación entre la enfermedad periodontal y la mortalidad en pacientes con enfermedad renal crónica en hemodiálisis.
TABLA DE CONTENIDO LUX MÉDICA 35
Authors: Terrones Saldívar Ma del Carmen
Published: March 2021

Presenta información general de la revista y su contenido.
Caso anatomopatológico: causa de muerte súbita en gente joven y revisión de la bibliografía
Authors: Bullón Alcalá Roberto , Sosa Morales Víctor , Montiel Esparza Ana Cecilia
Published: March 2021

La muerte súbita es una entidad poco conocida a nivel mundial, su incidencia se estima en el mundo entre 4 a 5 millones de casos. Es una entidad con varias causas y una de ellas es la displasia aloarritmogénica del ventrículo derecho, que es una enfermedad poco conocida en México por lo que se diagnostica poco; otras lesiones que causan esta entidad son la lipomatosis cardiaca, el síndrome de Brugada, el síndrome de UHL y el síndrome de QT largo, entre otros. En el presente trabajo describiremos brevemente estas enfermedades.En general, los casos de muerte súbita detectados son poco estudiados en la literatura médica debido a que su presentación y el impacto familiar no permiten dilucidar la causa exacta de la muerte. En México, las principales causas de muerte súbita que encontramos en los registros de defunción de diferentes delegaciones y estados de la república, se clasifican como infarto al miocardio, paro cardiorrespiratorio de causa no conocida o hemorragia cerebral. Esto solo para cumplir con un requisito desde el punto de vista jurídico; pero, en realidad, no se ha llevado a cabo un estudio sistemático para dilucidar las causas que producen la muerte súbita en personas jóvenes, como es nuestro caso, así como en sujetos adultos.
ÍNDICE DE AUTORES 2017
Authors: DE AUTORES INDICE
Published: March 2021

Reconocimiento a los autores que participaron en los números de la revista Lux Médica 2017. Muchas gracias a todos ellos.
The Effectiveness Of Vernonia Amygdalina (African Bitter Leaf) Tea For Reducing Cholesterol Levels In Individuals With Hypercholesterolemia
Authors: Purwanti Nunik , Aditya Rahmadiar
Published: March 2021

Advances in technology and high activities at work cause a person to prefer fast food, increasing the risks of high cholesterol levels. Meanwhile, the healing of hypercholesterolemia requires a long treatment time. One of them is non-pharmacological treatment using Vernonia amygdalina (African bitter leaf) tea. This study analyzes V.amygdalina (African bitter leaf) effectiveness for reducing the cholesterol levels in individuals with hypercholesterolemia. The design of this study was a quasi-experimental design, with a non-equivalent control group design. The population involved individuals with hypercholesterolemia in the village of Kedensari RW 05 Tanggulangin, Sidoarjo. There were 40 respondents in this study using the purposive sampling technique – 20 respondents in the experimental group and 20 respondents in the control group. The independent variable was V.amygdalina tea, whereas the dependent variable was cholesterol levels. The data were analyzed using the Wilcoxon and Mann-Whitney tests. The p-value of the Wilcoxon test and Mann-Whitney signed-ranks test was (0.000)<α(0.005), which illustrated that V.amygdalina (African bitter leaf) tea was useful for the treatment of hypercholesterolemia. In conclusion, tea made from African bitter leaf affects in reducing cholesterol levels. Therefore, this paper recommends using V.Amygdalina in other management forms besides tea because of its rich benefits as an alternative non-pharmacological hypercholesterolemia treatment.
La fi d'una llegenda: Arnau de Vilanova davant la màgia i l'astrologia
Authors: Giralt Sebastià
Published: March 2021

This article revises Arnau’s thoughts and practice regard-ing magic and astrology, two core aspects in the creation of an enduring legendary image. If apocryphal works are disregarded, the attitudes he sustains in his writing are in line with those of contemporary intellectuals: a rejec-tion of necromancy coupled with the acceptance of oc-cult astral properties as the foundation of natural magic. These properties are put to use in his medical treatises, where astrology is also the basis for some remedies. However, none of the treatises on medical astrology that have been transmitted under his name are his.
Система соціально-психологічного забезпечення процесу реінтеграції молоді в умовах сучасного соціуму
Authors: Блискун Олена
Published: March 2021

У статті визначено концептуальні засади системи соціально-психологічного забезпечення процесу реінтеграції молоді в умовах сучасного соціуму. Розкрито змістовно-процесуальні засади побудови і реалізації системи соціально-психологічного забезпечення процесу реінтеграції молоді в умовах сучасного соціуму, основу якої складала інтегративно-інваріантна технологія розвитку соціально-психологічної спроможності молоді, яка спирається на теорії соціалізації, когнітивно-біхевіоральний, системно-інтегративний та генетико-моделюючий підходи і включає методи корекції скриптів в процесі активного соціально-психологічного навчання, самоперепрограмування і творчість, як свідоме оформлення цілісного сценарію власного життя. На основі сукупності критеріїв і показників ефективності процесу реінтеграції виділені і описані рівні соціально-психологічного забезпечення цього процесу. На їх основі виявлено типологічні особливості груп респондентів, що сприяють підвищенню їх соціального потенціалу, створенню умов для успішної соціокультурної адаптації та процесу реінтеграції загалом, вибору напрямку діяльності у роботі з кожною з груп, визначення найбільш ефективних засобів і методів на основі використання методичної, ресурсної та організаційної складових запропонованої системи. Доведено, що впровадження системи соціально-психологічного забезпечення процесу реінтеграції молоді в умовах сучасного соціуму дозволяє значно підвищити соціально-психологічну спроможність молоді та ефективність цього процесу, оскільки забезпечує більш інтенсивний розвиток стилю саморегуляції поведінки і її компонентів; загального рівня антиципації і її складових; формування адекватної самооцінки; просоціальної переорієнтації екзистенційних цінностей, оптимальної й позитивної корекції життєвих цілей, зміни моральних настановлень та реконструкції свідомості і самосвідомості; сприяє позитивній корекції деструктивних соціальних установок і особистісних розладів у молоді. Встановлено обернено-пропорційний звʼязок між віком і ефективністю інтегративно-інваріантної технології та виявлено, що заходи системного впливу були більш ефективними для групи соціально-психологічно неспроможних респондентів зі зниженим соціальним потенціалом у порівнянні з групою респондентів з низьким соціальним потенціалом.
Ключові слова: молодь, реінтеграція, соціально-психологічне забезпечення процесу реінтеграції, сучасний соціум.
Сутність та структурапрофесійно важливих якостей: аналітичний огляд проблеми
Authors: Вовк Неля , Мохнар Людмила , Чередниченко Тетяна
Published: March 2021

У статті аналізуються погляди різних дослідників на сутність професійно важливих якостей фахівців, уточнюється їх зміст та структура, проаналізовано та систематизовано наукові дослідження проблематики розвитку та виховання ПВЯ з метою конкретизації терміну «Професійно важливі якості», уточнено їх сутність та структуру.
Проведено аналіз наукової літератури з проблеми розвитку та виховання ПВЯ фахівців у різних галузях професійної діяльності завдяки ґрунтовним розвідкам В.П. Бута, М.С. Корольчука, Г.В. Ложкіна, В.Л. Марищука, С.М. Миронця, О.Р. Орхеменко, О.О. Сегеди, В.Д. Шадрікова та ін. Розкрито категорію «діяльність», що знайшла своє відображення у таких науках як психологія, філософія, педагогіка, тощо.
У роботі знайшли відображення окремі питання розвитку та формування професійно важливих якостей та проаналізовано цілу низку класифікацій, за якими дослідники систематизують ПВЯ, і в основу яких покладені різні класифікаційні ознаки.
На підставі результатів проведеного аналізу поглядів різних науковців і фахівців, з’ясовано, що під професійно важливими якостями фахівця розуміють конкретні індивідуальні якості особистості, наявність яких забезпечує ефективне виконання професійних завдань.
Виходячи з цього, слід наголосити, що система психологічного забезпечення професійної діяльності забезпечує формування, збереження та підтримку необхідної працездатності фахівця, що, насамперед, залежить від сформованості та розвитку низки специфічних професійно важливих якостей.
Треба також відмітити, що незважаючи на те, що питання сутності професійно важливих якостей досліджується вже тривалий час, воно і тепер залишається актуальним, особливо у межах психології діяльності в особливих умовах. Це обумовлено, у першу чергу, впровадженням новітніх технологій у діяльність людини, що з’являються як результати досягнень світового науково-технічного прогресу, а також можливим виникненням небезпек техногенного, природного та воєнного характеру.
Ключові слова: діяльність, професійна діяльність, професійно важливі якості, професійні вимоги.
Соціально-психологічні ефекти особистісно-професійної адаптації фахівців соціономічного профілю
Authors: Жигаренко Ігор
Published: March 2021

У статті проведено аналіз підходів до проблеми дослідження показав, що у сучасних умовах динамічного розвитку суспільства проблема адаптації особистості до умов професійної діяльності продовжує актуалізуватися, наповнюється новим змістом і вивчається у традиціях субʼєктного, системного і комплексного підходів, екопсихології розвитку, що підкреслює багатофакторність і багатовимірність цього процесу. Показано, що в залежності від індивідуально-типологічних і соціально-психологічних властивостей особистості адаптаційний процес може відбуватися в активно-цілеспрямованій та у пасивно-пристосувальній формах. При цьому адаптація має на увазі як самореалізацію особистості, її адекватне функціонування, так і вираженість відхилень. Наголошується, що при несприятливому збігу соціальних умов, в періоди вікових та професійних криз спостерігається дезадаптація фахівця, яка виходить на особистісний рівень його функціонування. З цих позицій виокремлюють обʼєктивні (результативність і якість діяльності) і субʼєктивні (професійне самовизначення (ступінь прийняття себе як професіонала, усвідомлення рівня розвитку своїх здібностей, компетентності, мотивів), задоволеність діяльністю (ступінь прийняття умов і змісту діяльності)) критерії ефективності адаптаційного процесу. Підкреслюється, що негативні зміни особистості фахівця в процесі адаптації, оволодіння «брудними» стандартами і цінностями професійного співтовариства, реалізація негативних аспектів накопиченого професійного досвіду, відтворення стереотипних соціальних комунікацій, є особливо небезпечними для професійної діяльності соціономічного типу, що артикулює необхідність грунтовного вивчення соціально-психологічних основ особистісно-професійної
Ключові слова: фахівці соціономічного профілю, особистісно-професійна адаптація, соціально-психологічні параметри особистісно-професійної адаптації, соціально-психологічні ресурси особистісно-професійної адаптації.
Gated SPECT Myocardial Perfusion Imaging in female patients - an observational study
Authors: Sarker Azmal K , Naznin Afroza , Sultana Nasreen , Nasreen Faria , Nisa Lutfun
Published: March 2021

Background: It had been a general observation that the number of female patients undergoing SPECT myocardial perfusion imaging (MPI) is lower per week.This study was done to find out their proportionand association among their symptoms, history of (h/o) acute coronary syndrome (ACS) and corresponding myocardial perfusion.

Patients and methods:This cross sectional retrospective study was conducted in 2017 on a group of patients who underwent gated SPECT myocardial perfusion imaging (GSMPI)from February 2005 to October 2016. Clinical record files and image files of all female patients were selected for analysis. The symptoms were retrospectively categorized according to New York Heart Association (NYHA) class while the patients were categorized according to their h/o ACS and myocardial perfusion in each patient was categorized as normal or perfusion defect. Data were analyzed using SPSS v.20.

Results:Among 1347 patients undergoing GSMPI 196 (14.6%) were female with a 4.4-fold increase in number over 12 years. Mean age was 51.8 ± 10.1 (27-80). Symptom categorization assigned 84 patients in NYHA class I and 112 in NYHA class II. There was no h/o ACS in 143 while 53 had a previous h/o hospitalization due to ACS. GSMPI revealed normal perfusion in 156 patients with mean left ventricular ejection fraction (LVEF) of 66.6 ± 13.6. Perfusion defect was present in 40 patients with mean LVEF of 45.5 ± 15.8. NYHA class of symptoms was not associated with perfusion status (p > 0.05).H/o ACS was found to have association both with NYHA class of symptoms and with perfusions status (p < 0.05).

Conclusions: Proportion of female patients undergoing GSMPI is low. Presence of perfusion defect in female was not associated with NYHA class of symptom.

Bangladesh J. Nuclear Med. 22(2): 103-107, Jul 2019
Pelvic Kidney Mimicking Skeletal Metastasis on Bone Scan- Interesting Image
Authors: Bhuiyan Mohammed Mehedi Al Zahid , Sarker Azmal Kabir , Choi Hongyoon , Suh Minseok , Cheon Gi Jeong
Published: March 2021

A 57-year-old female patient underwent left breast-conserving surgery with sentinel lymph node biopsy for Left breast carcinoma (stage IIA). The patient had hypertension and diabetes mellitus. Other findings include multiple hepatic cyst, bilateral renal cysts and uterine myoma. She had no significant renal symptoms and her liver &renal function test were normal.She was sent for Technetium-99m-methylene diphosphonate (99mTc-MDP) bone scan. There was a large area of intense tracer concentration in the region of right sacro-iliac (SI) joint which appeared like an osteoblastic metastasis at first glance. However, absence of uptake in the right renal fossa with the left kidney being normal in position contemplated the probability of right-sidedpelvickidneywhich was confirmed later by a contrast enhanced computerized tomography (CT) scan of abdomen that showed a pelvic right kidney overlying the sacrum.

Bangladesh J. Nuclear Med. 22(2): 155-156, Jul 2019
O ativismo judicial é um jogo sem fair play? Direito e Brincadeira
Authors: Medina Janaina de Castro Marchi , Toledo Renata Maria Silveira
Published: March 2021

O presente artigo tem por objetivo analisar o fenômeno do ativismo judicial no jogo do Direito, cuja relevância justifica-se pela crise democrática instalada no Brasil. Para tanto, houve a preocupação em se fazer um resgate histórico do seu contexto, a diferenciação entre ativismo judicial e judicialização de políticas públicas, e o enfrentamento de duas posições doutrinárias antagônicas: a de Luís Roberto Barroso e a de Lenio Luiz Streck. O trabalho ainda permeia discussões sobre a vulnerabilidade da democracia e as regras de seu jogo, impactados pela aplicação de um “ativismo” às avessas do Poder Executivo e decisões judiciais ativistas proferidas no momento em que passa por pandemia do COVID-19, fundadas em valores pessoais de seus respectivos representantes institucionais, que atuam como intérpretes constitucionais. A pesquisa utilizou-se da metodologia dedutiva por meio de pesquisa bibliográfica, incluindo autores nacionais e estrangeiros, além de decisões judiciais recentes que demonstram como se está lidando com a atual crise sanitária.
Os desafios da enfermagem obstétrica no início da pandemia da COVID-19 no Estado do Pará
Authors: Costa Tais Pereira da , Ferreira Elisângela da Silva , Rodrigues Diego Pereira , Neri Débora Thalita , Soares Elannira Amaral , Ferreira Gabriela Campos de Freitas , Araújo Rafaela Moura de
Published: March 2021

Este estudo teve por objetivo relatar a experiência sobre os desafios da enfermagem obstétrica no contexto do início da pandemia da COVID-19 no Estado do Pará. Trata-se de um estudo descritivo do tipo relato de experiência realizado durante a pandemia do novo Coronavírus, sobre a atenção obstétrica prestada, dentro do período de março a agosto de 2020. Dentro dos resultados destaca-se que a partir dos boletins epidemiológicos, foi demonstrado que dentre os estados que mais concentraram óbitos de gestantes por COVID-19, o Pará ocupa o 6º lugar com 08 casos registrados, até o dia 01/08/2020. Atuando no campo obstétrico durante a pandemia, nos meses iniciais e críticos da doença no Brasil, observaram-se várias dificuldades e entraves no fluxo e estrutura do atendimento obstétrico nos campos de atuação da enfermagem. Verificou-se inúmeros afastamentos de profissionais, por serem de grupo de risco, e até mesmo, a interrupção de atendimentos de pré-natal, com cancelamento das consultas, durante a fase complicada, de altos índices da doença. Deste modo, deve-se refletir os desafios que o profissional enfermeiro enfrentou e deve superar ao vivenciar a pandemia da COVID-19 no que diz respeito à sua assistência obstétrica.
Volunteer’s willingness to genetic testing - lack of the understanding of the matter
Authors: Adámková V , Veleminský M , Zimmelová P , Hubáček JA
Published: March 2021

Medical genetic research achieved in last decade many efforts
leading to better understanding of inherited basis of human
diseases. This will not be possible without the participation of
patients and controls. However, the general understanding of the
background and possibilities of genetic association studies is
very low. It was confirmed by study of university of students.
Because of the fair of misuses of the individual genetic
information, significant part of participants refused the use of
already donated blood samples for genetic testing but agreed
with using of buccal swabs for the same analysis. To enhance the
general knowledge’s of the population, leading to realistic
expectations not just about genetic predictive power but also
about the eventual risks in behind will be the major mission in
the next years.
SISTEM PENGOLAHAN DATA JEMAAT GKJ KISMOREJO BERBASIS WEB
Authors: Aji Setiawan Oktavianus Bima , WINARSIH S.M SANTI
Published: March 2021

The problems faced by GKJ Kismorejo currently are still using Microsoft Office Word and in disseminating information such as data on congregations that are still active or not active, information on congregations who died, congregational marriages, and other congregational data through paper-based congregations. To print church messages and reports requires a large amount of operational funds. In addition, data collection and storage still have shortcomings, including recording some data in books, difficulties in processing large amounts of data and storing it in only one place. For this reason, the authors raise the background of the problems that occur, the authors are interested in conducting research with the title Designing Data Processing Systems for Web-Based GKJ Kismorejo Congregation. The purpose of this research is to design and produce a data processing system for the church. Using this system can facilitate data processing in the church.

Keywords: web, data processing, operational

Permasalahan yang dihadapi GKJ Kismorejo saat ini masih menggunakan Microsoft Office Word dan dalam penyebaran informasi seperti data jemaat yang masih aktif atau sudah tidak aktif, informasi jemaat meninggal dunia, pernikahaan jemaat, dan data jemaat lainnya melalui warta jemaat yang berbasis kertas. Untuk mencetak warta jemaat dan laporan membutuhkan dana operasional cukup besar. Selain itu pengumpulan dan penyimpanan data masih terdapat kekurangan di antaranya mencatat beberapa data pada buku, kesulitan untuk mengolah data dalam jumlah besar dan tersimpan hanya pada satu tempat. Untuk itu penulis mengangkat latar belakang dari permasalahan yang terjadi, penulis tertarik untuk melakukan penelitian dengan judul Perancangan Sistem Pengolahan Data Kegiatan Jemaat GKJ Kismorejo Berbasis Web. Tujuan penelitian ini untuk merancang dan menghasilkan suatu sistem pengolahan data untuk gereja. Dengan menggunakan sistem ini dapat memudahkan dalam  pengolahan data dalam gereja.

Kata kunci : web, pengolahan data,operasional
Application of Inquiry Learning Model in Improving Class VII Students Achievement at St. Catholic High School Aloysius Wolotopo
Authors: Mbia Wae Veronika Praja Sinta
Published: March 2021

The purpose of this study was to determine the effect of the application of the inquiry learning model in improving student achievement in the seventh grade plant organ material at St. Catholic Middle School. Aloysius Wolotopo. This research is a Classroom Action Research which is conducted in 2 cycles, where each cycle consists of planning, acting, observing and reflecting. The subjects of this study were students of class VII SMPS Catholic St. Aloysius Wolotopo for the 2019/2020 school year, amounting to 31 people. Data sourced from teachers and students. The data collection technique was done by means of tests and non-tests (observation). Data analysis using qualitative descriptive analysis techniques. The results showed that the application of the inquiry learning model could improve student achievement in plant organ material. This can be seen from the results of the study, namely an increase in the percentage of completeness from cycle I by 61% to 100% in cycle II. Student participation in the learning process also increased from 53% in cycle I to 90% in cycle II.
A study of the hagioscopes in northern England and their meaning for pre Reformation worship.
Authors: Henders Stephen
Published: March 2021

Study of hagioscopes in medieval settings considering the concept of regular worship and special Easter devotions. drawing particular attention to isolation and social distancing. This study also explores the use of the hagioscope as a form of social control and covert surveillance through a form of close visual and aural monitoring.
Cord Lining Mesenchymal Stem Cells Have a Modest Positive Effect on Angiogenesis in Hindlimb Ischemia
Authors: Chua Kenon , Lim Fui Ping , Lee Victor Kwan Min , Phan Toan Thang , Tai Bee Choo , Tan Yih Kai
Published: March 2021

Purpose: We investigated the use of human Cord Lining Mesenchymal Stem Cells (CL-MSCs) (US Patent number 9,737,568), in a rabbit hindlimb ischemia model, and evaluated their potential in stimulating neovascularization. Allogenic human CL- MSCs could potentially be used to treat patients with lower limb ischemia and non-healing wounds.

Methods: Twenty rabbits were divided into two separate groups. We created a hindlimb ischemia model surgically. At 21 and 49 days post-operatively, animals in the treatment group were injected with CL-MSCs (500,000 cells per 0.2 ml on each site) at 10 different sites (Quadriceps- 4 sites, Hamstrings- 4 sites and Calf-−2 sites) in the hindlimb muscles. The control group received only saline injection to the corresponding sites at the same time point as the treatment group. We then evaluated the effects of treatment on neovascularization by angiography, laser doppler perfusion imaging, as well as by histology. We evaluated the tissue samples for any signs of local immune reaction to the cell implantation. We also observed the rabbit clinically for any adverse effects after treatment.

Results: We found a higher number of CD31 positive cells in the treatment group, with a greater number of capillaries found in the treated muscles. The Rectus Femoris demonstrated a median vessel count/muscle fiber of 0.121 for the treatment group, compared to 0.076 in the control group (median difference 0.04; 95% CI 0.001–0.11; p = 0.041). The Gastrocnemius demonstrated a median vessel count/muscle fiber of 0.175 for the treatment group, compared to 0.089 in the control group (median difference 0.087; 95% CI −0.006 to 0.234; p = 0.07). Blood perfusion quantification through Laser Doppler Perfusion Imaging (LDPI) also demonstrated a non-statistically significant increase in perfusion in favor of the treatment group. CL-MSCs demonstrated no toxicity associated morbidity and minimal local immune reaction to implantation.

Conclusion: CL-MSCs have a positive effect on angiogenesis in a rabbit hindlimb ischemia model. This preliminary data is encouraging and paves the way for future large animal studies or for clinical trials.
Discovery of New α-Glucosidase Inhibitors: Structure-Based Virtual Screening and Biological Evaluation
Authors: Liu Shan-Kui , Hao Haifang , Bian Yuan , Ge Yong-Xi , Lu Shengyuan , Xie Hong-Xu , Wang Kai-Ming , Tao Hongrui , Yuan Chao , Zhang Juan , Zhang Jie , Jiang Cheng-Shi , Zhu Kongkai
Published: March 2021

α-Glycosidase inhibitors could inhibit the digestion of carbohydrates into glucose and promote glucose conversion, which have been used for the treatment of type 2 diabetes. In the present study, 52 candidates of α-glycosidase inhibitors were selected from commercial Specs compound library based on molecular docking–based virtual screening. Four different scaffold compounds (7, 22, 37, and 44) were identified as α-glycosidase inhibitors with IC50 values ranging from 9.99 to 35.19 μM. All these four compounds exerted better inhibitory activities than the positive control (1-deoxynojirimycin, IC50 = 52.02 μM). The fluorescence quenching study and kinetic analysis revealed that all these compounds directly bind to α-glycosidase and belonged to the noncompetitive α-glycosidase inhibitors. Then, the binding modes of these four compounds were carefully investigated. Significantly, these four compounds showed nontoxicity (IC50 > 100 μM) toward the human normal hepatocyte cell line (LO2), which indicated the potential of developing into novel candidates for type 2 diabetes treatment.
Association of Parkinson’s Disease With Microbes and Microbiological Therapy
Authors: Chen Zhao-Ji , Liang Cheng-Yu , Yang Li-Qing , Ren Si-Min , Xia Yan-Min , Cui Lei , Li Xiao-Fang , Gao Bu-Lang
Published: March 2021

Parkinson’s disease (PD) is the most common movement disorder in the world, affecting 1–2 per 1,000 of the population. The main pathological changes of PD are damage of dopaminergic neurons in substantia nigra of the central nervous system and formation of Lewy bodies. These pathological changes also occur in the intestinal tract and are strongly associated with changes in intestinal flora. By reviewing the research progress in PD and its association with intestinal flora in recent years, this review expounded the mechanism of action between intestinal flora and PD as well as the transmission mode of α - synuclein in neurons. In clinical studies, β diversity of intestinal flora in PD patients was found to change significantly, with Lactobacillusaceae and Verrucomicrobiaceae being significantly increased and Lachnospiraceae and Prevotellaceae being significantly decreased. In addition, a longer PD course was associated with fewer bacteria and probiotics producing short chain fatty acids, but more pathogenic bacteria. Moreover, the motor symptoms of PD patients may be related to Enterobacteriaceae and bacteria. Most importantly, catechol-O-methyltransferase inhibitors and anticholinergic drugs could change the intestinal flora of PD patients and increase the harmful flora, whereas other anti-PD drugs such as levodopa, dopamine agonist, monoamine oxidase inhibitors, and amantadine did not have these effects. Probiotics, prebiotics, and synbiotics treatment had some potential values in improving the constipation of PD patients, promoting the growth of probiotics, and improving the level of intestinal inflammation. At present, there were only a few case studies and small sample studies which have found certain clinical efficacy of fecal microbiome transplants. Further studies are necessary to elaborate the relationship of PD with microbes.
Novel Endosymbionts in Rhizarian Amoebae Imply Universal Infection of Unrelated Free-Living Amoebae by Legionellales
Authors: Solbach Marcel Dominik , Bonkowski Michael , Dumack Kenneth
Published: March 2021

Legionellales-infected water is a frequent cause of local outbreaks of Legionnaires’ disease and Pontiac fever. Decontaminations are difficult because Legionellales reproduce in eukaryotic microorganisms (protists). Most often, Legionellales have been isolated from amoebae; however, the culture-based sampling methods are taxonomically biased. Sequencing studies show that amoebae in the cercozoan class Thecofilosea are dominant in soils and wastewater treatment plants, prompting us to screen their capability to serve as potential hosts of endosymbiotic bacteria. Environmental isolates of Thecofilosea contained a surprising richness of endosymbiotic Legionellales, including Legionella. Considering the widespread dispersal of Legionellales in apparently unrelated amoeboid protist taxa, it appears that the morphotype and not the evolutionary origin of amoebae determines their suitability as hosts for Legionellales. We further provide a protocol for gnotobiotic cultivation of Legionellales and their respective hosts, facilitating future genomic and transcriptomic research of host–symbiont relationships.
Meta-Analysis: Association Between Hypoglycemia and Serious Adverse Events in Older Patients Treated With Glucose-Lowering Agents
Authors: Mattishent Katharina , Loke Yoon K.
Published: March 2021

Aims

We conducted a meta-analysis of serious adverse events (dementia, macro- and micro-vascular events, falls and fractures, and death) associated with hypoglycemia in older patients treated with glucose lowering drugs.

Materials and Methods

Meta-analysis of studies reporting on hypoglycemia and adverse events. The search included studies from two previously published systematic reviews, and an updated search of MEDLINE and EMBASE from April 2014 to November 2019. We assessed study validity based on ascertainment of hypoglycemia, adverse events and adjustment for confounders, and conducted a random effects meta-analyses, assessing heterogeneity using the I2 statistic.

Results

We included 44 studies involving 2,507,434 participants. Most of the studies used adjusted analysis for confounders and hypoglycaemic events were typically identified based on healthcare databases (severe events). Hypoglycemia was associated with increased likelihood of death in a meta-analysis of eighteen studies, pooled OR 2.02 (95% Confidence Interval 1.75–2.32). Studies assessing mortality signal a time-response relationship with a higher risk of adverse events occurring within the first 90 days after hypoglycemia. Our meta-analysis of nine studies demonstrated that hypoglycaemic episodes were associated with dementia – pooled OR 1.50 (95% CI 1.29–1.74). Our meta-analysis of nineteen studies demonstrated associations between hypoglycaemia and macrovascular complications, pooled OR 1.81 (95% CI 1.70–1.94), and microvascular complications (two studies) pooled OR 1.77 (95% CI 1.49–2.10). There is also an association between hypoglycemia and cardiovascular death (six studies) – pooled OR 2.11 (95% CI 1.55 to 2.87). Similarly, our meta-analysis of six studies demonstrated an association between hypoglycemia and falls and fractures, pooled OR 1.78 (95% CI 1.44–2.21) and 1.68 (95% CI 1.37–2.07) respectively.

Conclusion

This meta-analysis confirms previously reported concerns of serious harm following hypoglycemia, especially in the immediate time period after a hypoglycaemic event. Avoidance of hypoglycaemic episodes should be a priority in this vulnerable population.
Improving Habitat Quality at the Local and Landscape Scales Increases Wild Bee Assemblages and Associated Pollination Services in Apple Orchards in China
Authors: Wu Panlong , Dai Piaopiao , Wang Meina , Feng Sijie , Olhnuud Aruhan , Xu Huanli , Li Xiang , Liu Yunhui
Published: March 2021

Bees provide key pollination services for a wide range of crops. Accumulating evidence shows the effect of semi-natural habitats at the landscape level and local management practices on bee diversity in fields. However, most of the evidence is derived from studies in North America and Europe. Whether this paradigm is applicable in China, which is characterized by smallholder-dominated agricultural landscapes, has rarely been studied. In this study, we aimed to investigate how bee diversity affected apple production, and how landscape and local variables affected bee diversity and species composition on the Northern China Plain. The results showed that bees significantly increased apple fruit set compared to bagged controls. Wild bee diversity was positively related to apple seed numbers. Higher seed numbers reduced the proportion of deformed apples and thus increased fruit quality. Wild bee abundance was positively correlated with flowering ground cover, and both the abundance and species richness of wild bees were positively affected by the percentage of semi-natural habitats. We conclude that apple quality can benefit from ecological intensification comprising the augmentation of wild bees by semi-natural habitats and flowering ground cover. Future pollination management should therefore reduce the intensification level of management at both the local and landscape scales.
Ketamine Inhibits Ovarian Cancer Cell Growth by Regulating the lncRNA-PVT1/EZH2/p57 Axis
Authors: Li Tao , Yang Jie , Yang Ben , Zhao Guoqing , Lin Hai , Liu Qi , Wang Leiming , Wan Yingchun , Jiang Hongyang
Published: March 2021

Ketamine is widely used for cancer pain treatment in clinic, and has been shown to inhibit various tumor cells growth. However, the effect of ketamine on ovarian cancer cells growth and the downstream molecules has not been defined. In the present study, we found that ketamine significantly inhibited the proliferation and survival of six ovarian cancer cell lines. Moreover, ketamine induced ovarian cancer cell cycle arrest, apoptosis, and inhibited colony formation capacity. Since lncRNAs have been identified as key regulators of cancer development, we performed bioinformatics analysis of a GEO dataset and found fourteen significantly altered lncRNAs in ovarian cancer patients. We then investigated the effect of ketamine on these lncRNAs, and found that ketamine regulated the expression of lncRNA PVT1. Mechanistically, ketamine regulated P300-mediated H3K27 acetylation activation in the promoter of PVT1. Our RNA immunoprecipitation experiment indicated that PVT1 bound histone methyltransferase enhancer of zeste homolog 2 (EZH2), and regulated the expression of target gene, including p57, and consequently altered ovarian cancer cell biology. Our study revealed that ketamine could be a potential therapeutic strategy for ovarian cancer patients.
Genetic Analysis of Persistency for Milk Fat Yield in Iranian Buffaloes (Bubalus bubalis)
Authors: Nazari Mohammad Ali , Ghavi Hossein-Zadeh Navid , Shadparvar Abdol Ahad , Kianzad Davood
Published: March 2021

This study aimed to estimate heritabilities and genetic trends for different persistency measures for milk fat yield and their genetic correlations with 270-day milk yield in Iranian buffaloes. The records of test-day milk fat yield belonging to the first three lactations of buffaloes within 523 herds consisting of 43,818 records were got from the Animal Breeding Center and Promotion of Animal Products of Iran from 1996 to 2012. To fit the lactation curves based on a random regression test-day model, different orders of Legendre polynomial (LP) functions were selected. Three persistency measures were altered according to the specific condition of the lactation curve in buffaloes: (1) The average of estimated breeding values (EBVs) for test day fat yield from day 226 to day 270 as a deviation from the average of EBVs from day 44 to day 62 (PM1), (2) A summation of contribution for each day from day 53 to day 247 as a deviation from day 248 (PM2), and (3) The difference between EBVs for day 257 and day 80 (PM3). The estimates of heritability for PM1, PM2, and PM3 ranged from 0.20 to 0.48, from 0.36 to 0.47, and from 0.19 to 0.35 over the first three lactations, respectively. The estimate of genetic trends for different persistency measures of milk fat yield was not significant over the lactations (P > 0.05). Genetic correlation estimates between various measures of persistency were generally high over the first three lactations. Also, genetic correlations estimates between persistency measures and 270-day milk yield were mostly low and varied from 0.00 to 0.24 (between PM1 and 270-day milk yield), from −0.19 to 0.13 (between PM2 and 270-day milk yield), and from −0.02 to 0.00 (between PM1 and 270-day milk yield) over the first three lactations, respectively. Persistency measures that showed low genetic correlations with milk fat yield were considered the most suitable measures in selection schemes. Besides, medium to high heritability estimates for different persistency measures for milk fat yield indicated that relevant genetic variations detected for these characters could be regarded in outlining later genetic improvement programs of Iranian buffaloes.
Spondyloarthritis and the Human Leukocyte Antigen (HLA)-B*27 Connection
Authors: Kavadichanda Chengappa G. , Geng Jie , Bulusu Sree Nethra , Negi Vir Singh , Raghavan Malini
Published: March 2021

Heritability of Spondyloarthritis (SpA) is highlighted by several familial studies and a high association with the presence of human leukocyte antigen (HLA)-B*27. Though it has been over four decades since the association of HLA-B*27 with SpA was first determined, the pathophysiological roles played by specific HLA-B*27 allotypes are not fully understood. Popular hypotheses include the presentation of arthritogenic peptides, triggering of endoplasmic reticulum (ER) stress by misfolded HLA-B*27, and the interaction between free heavy chains or heavy chain homodimers of HLA-B*27 and immune receptors to drive IL-17 responses. Several non-HLA susceptibility loci have also been identified for SpA, including endoplasmic reticulum aminopeptidases (ERAP) and those related to the IL-23/IL-17 axes. In this review, we summarize clinical aspects of SpA including known characteristics of gut inflammation, enthesitis and new bone formation and the existing models for understanding the association of HLA-B*27 with disease pathogenesis. We also examine newer insights into the biology of HLA class I (HLA-I) proteins and their implications for expanding our understanding of HLA-B*27 contributions to SpA pathogenesis.
ADP-Ribosylation Regulates the Signaling Function of IFN-γ
Authors: Menzel Stephan , Koudelka Tomas , Rissiek Björn , Haag Friedrich , Meyer-Schwesinger Catherine , Tholey Andreas , Koch-Nolte Friedrich
Published: March 2021

Murine T cells express the GPI-anchored ADP-ribosyltransferase 2.2 (ARTC2.2) on the cell surface. In response to T cell activation or extracellular NAD+ or ATP-mediated gating of the P2X7 ion channel ARTC2.2 is shed from the cell surface as a soluble enzyme. Shedding alters the target specificity of ARTC2.2 from cell surface proteins to secreted proteins. Here we demonstrate that shed ARTC2.2 potently ADP-ribosylates IFN-γ in addition to other cytokines. Using mass spectrometry, we identify arginine 128 as the target site of ADP-ribosylation. This residue has been implicated to play a key role in binding of IFN-γ to the interferon receptor 1 (IFNR1). Indeed, binding of IFN-γ to IFNR1 blocks ADP-ribosylation of IFN-γ. Moreover, ADP-ribosylation of IFN-γ inhibits the capacity of IFN-γ to induce STAT1 phosphorylation in macrophages and upregulation of the proteasomal subunit ß5i and the proteasomal activator PA28-α in podocytes. Our results show that ADP-ribosylation inhibits the signaling functions of IFN-γ and point to a new regulatory mechanism for controlling signaling by IFN-γ.
Hotter and Weaker Mediterranean Outflow as a Response to Basin-Wide Alterations
Authors: García-Lafuente Jesús , Sammartino Simone , Huertas I. Emma , Flecha Susana , Sánchez-Leal Ricardo F. , Naranjo Cristina , Nadal Irene , Bellanco María Jesús
Published: March 2021

Time series collected from 2004 to 2020 at an oceanographic station located at the westernmost sill of the Strait of Gibraltar to monitor the Mediterranean outflow into the North Atlantic have been used to give some insights on changes that have been taking place in the Mediterranean basin. Velocity data indicate that the exchange through the Strait is submaximal (that is, greater values of the exchanged flows are possible) with a mean value of −0.847 ± 0.129 Sv and a slight trend to decrease in magnitude (+0.017 ± 0.003 Sv decade−1). Submaximal exchange promotes footprints in the Mediterranean outflow with little or no-time delay with regards to changes occurring in the basin. An astonishing warming trend of 0.339 ± 0.008°C decade−1 in the deepest layer of the outflow from 2013 onwards stands out among these changes, a trend that is an order of magnitude greater than any other reported so far in the water masses of the Mediterranean Sea. Biogeochemical (pH) data display a negative trend indicating a gradual acidification of the outflow in the monitoring station. Data analysis suggests that these trends are compatible with a progressively larger participation of Levantine Intermediate Water (slightly warmer and characterized by a pH lower than that of Western Mediterranean Deep Water) in the outflow. Such interpretation is supported by climatic data analysis that indicate diminished buoyancy fluxes to the atmosphere during the seven last years of the analyzed series, which in turn would have reduced the rate of formation of Western Mediterranean Deep Water. The flow through the Strait has echoed this fact in a situation of submaximal exchange and, ultimately, reflects it in the shocking temperature trend recorded at the monitoring station.
Phage-Mediated Control of Flavobacterium psychrophilum in Aquaculture: In vivo Experiments to Compare Delivery Methods
Authors: Donati Valentina Laura , Dalsgaard Inger , Sundell Krister , Castillo Daniel , Er-Rafik Mériem , Clark Jason , Wiklund Tom , Middelboe Mathias , Madsen Lone
Published: March 2021

Phage-based approaches have gained increasing interest as sustainable alternative strategies to antibiotic treatment or as prophylactic measures against disease outbreaks in aquaculture. The potential of three methods (oral, bath, and injection) for delivering a two-component phage mixture to rainbow trout fry for controlling Flavobacterium psychrophilum infections and reduce fish mortality was investigated using bacteriophages FpV4 and FPSV-D22. For the oral administration experiment, bacteriophages were applied on feed pellets by spraying (1.6 × 108 PFU g–1) or by irreversible immobilization (8.3 × 107 PFU g–1), using the corona discharge technology (Fixed Phage Ltd.). The fish showed normal growth for every group and no mortality was observed prior to infection as well as in control groups during the infection. Constant detection of phages in the intestine (∼103 PFU mg–1) and more sporadic occurrence in kidney, spleen, and brain was observed. When fish were exposed to F. psychrophilum, no significant effect on fish survival, nor a direct impact on the number of phages in the sampled organs, were detected. Similarly, no significant increase in fish survival was detected when phages were delivered by bath (1st and 2nd bath: ∼106 PFU ml–1; 3rd bath: ∼105 PFU ml–1). However, when phages FpV4 and FPSV-D22 (1.7 × 108 PFU fish–1) were administered by intraperitoneal injection 3 days after the bacterial challenge, the final percent survival observed in the group injected with bacteriophages FpV4 and FPSV-D22 (80.0%) was significantly higher than in the control group (56.7%). The work demonstrates the delivery of phages to fish organs by oral administration, but also suggests that higher phage dosages than the tested ones may be needed on feed pellets to offer fish an adequate protection against F. psychrophilum infections.