bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatio-temporal evolution and prediction of carbon storage at the source of the Fen River and Sanggan River based on a PLUS-InVEST model
Authors: Fu Shaotong , Zhen Zhilei , Zhou Haoyan , Wang Ben , Qiao Qiong
Published: November 2024

Investigating the evolution of land use and its impact on carbon storage is of significant importance for mitigating regional climate change and promoting green low-carbon development. Ningwu County is located in the source region of the Fenhe and Sanggan River, and its ecological status significantly influences the carbon storage (CS) of the watershed ecosystem. In this study, the PLUS-InVEST model was employed to analyze the land use evolution from 1990 to 2020 in Ningwu County, Shanxi Province, as well as their impacts on CS. Additionally, the study simulated and predicted land use changes in Ningwu County by 2040 under four scenarios: natural development (NDS), ecological protection (EPS), cultivated land protection (CLPS), and urban development (UDS), while estimating the corresponding changes in ecosystem CS. Furthermore, the study utilized optimal parameters-based geographical detector to explore the mechanisms underlying the spatial differentiation of CS. The results indicated that the areas of forest land and construction land in the study area consistently increased from 1990 to 2020, whereas the area of cultivated land continuously declined, with grassland, water bodies, and unused land exhibiting a fluctuating increasing trend. The spatial distribution of CS was highest in the northwest, second highest in the southeast, and lowest in the middle region. Over these 3 decades, CS had shown a continuous increase. It is projected that by 2040, the areas of forest and grassland will experience the most significant increase under the EPS; cultivated land only increase under the CLPS; while construction land display the greatest increase under the UDS. Compared to 2020, these four scenarios for 2040 indicate an increase in regional CS, with the EPS showing the largest increment. The primary factors influencing the spatial differentiation of CS in Ningwu County are human activities, followed by topography and climate change; the interactions among these factors exhibit a reinforcing relationship, with the interaction between the distance from construction land and slope having the most substantial impact on the spatial differentiation of CS.
Evaluating China’s urbanization trajectory: an overextension or still in progress?
Authors: Wei Yujun , Yuan Fan , Ye Zhonghua
Published: November 2024

China’s urbanization has undergone a transformative journey since the initiation of the reform and opening-up policy in 1978, catalyzing economic growth while profoundly impacting its ecological and demographic landscapes. This study offers a systematic evaluation of China’s urbanization trajectory over the past 4 decades through the development of an indicator system encompassing three key dimensions: population urbanization, land urbanization, and economic urbanization. Using the entropy method for weight assignment, the analysis reveals a consistent upward trend in China’s comprehensive urbanization, with occasional sharp increases. Although population urbanization influences the overall index, land urbanization has outpaced both population and economic urbanization, indicating a pronounced dependence on land resources. This trend poses a potential risk of unsustainable urban growth if left unaddressed. The findings indicate that to achieve sustainable urban development, China must prioritize balancing the interplay between population and land, moving away from land-dependent strategies, and adopting a more balanced approach to urbanization. The quantitative analysis provides key insights into this imbalance, indicating that policies should integrate land use planning with population dynamics to promote holistic urban growth. Future research should investigate the long-term socio-economic and environmental impacts of land-centric urbanization and propose innovative strategies to guide China towards a sustainable urban future.
Rainwater harvesting technologies in arid plains of Argentina: small local strategies vs. large centralized projects
Authors: Calderón Archina Aldana , Escolar Diego , Heider Guillermo , Niborski Marcos J. , Jobbágy Esteban G. , Magliano Patricio N.
Published: November 2024

Access to water has been and remains one of humanity’s greatest challenges. Especially in arid plains exposed to significant climatic fluctuations and future global change trends. In the past and present, local communities of the arid plains of central-western Argentina (i.e., Guanacache Lagoons, Cuyo region) have developed multiple strategies to manage water supply problems. The aims of this study are: i) to characterize the different water harvesting technologies (pre-Hispanic and modern) used, and ii) to compare the small local strategies of water harvesting (bottom-up solutions) with the large centralized projects (top-down solutions). On the one hand, we show the transformations of these technologies over time, and the challenges faced by inhabitants in the context of climate change trends. On the other hand, we analyze the role of the state through hydraulic policies and projects implemented by the provincial states over the last two centuries and how this impacted the study area. This review is based on a historical and archaeological bibliography, and recent publications about the region, including articles based on our ethnographic fieldwork. Our results demonstrate the valuable experience accumulated by local populations in water harvesting methods, particularly in areas where groundwater is deep and saline, and shows the adaptability of these technologies in contexts of increasing scarcity. We considered that local indigenous knowledge can largely contribute to the sustainable management of water resources. This study might be useful for decision-makers and water managers in drylands around the world to find and equitable approach that combines technical advances with local knowledge.
Research on the coupled evolution of LULCC and habitat quality in the Ganqing section of the Yellow River Basin based on multi-scenario simulations
Authors: Yang Jie , Xie Baopeng , Zhou Jie
Published: November 2024

Introduction

This study focuses on the Ganqing section of the Yellow River Basin, exploring four land use scenarios: natural development, cropland protection, ecological protection, and rapid development. Given the ecological importance of this area, the research aims to evaluate how each scenario impacts habitat quality and land use sustainability by 2030.

Methods

The Future Land Use Simulation (FLUS) model and the Integrated Valuation of Environmental Services and Tradeoffs (InVEST) model were applied to simulate land use for each scenario. A habitat quality pattern and coupling coordination degree model was used to assess the interactions between land use and land cover change (LULCC) and habitat quality under different scenarios.

Results

Findings show that over 70% of the Ganqing section of the Yellow River Basin is primarily grassland. By 2030, the ecological protection scenario is predicted to have the highest habitat quality, followed by the natural development, rapid development, and cropland protection scenarios. Between 1990 and 2030, the area demonstrates predominantly high or moderate coordination between land use and habitat quality. Spatial analysis reveals lower coordination values in the southeast and higher values in the northwest, with imbalanced recession zones distributed around valley basins.

Discussion

This study highlights the value of strategic scenario planning in enhancing habitat quality and promoting sustainable land management in the Ganqing section of the Yellow River Basin. The ecological protection scenario shows the most promise for balancing development with habitat preservation, underscoring the importance of adopting land use policies that support ecological sustainability in vulnerable areas.
Comparison of antibody responses of heterologous and homologous Covid-19 booster vaccination: an observational study
Authors: Rizvi Nayab Batool , Bibi Maryam , Rana Muhmmad Zeeshan , Zaffar Sehrish , Farooq Hassam
Published: November 2024

Objective

Pakistan has been seriously affected by the COVID-19 pandemic, with numerous waves of infection. Using different vaccine and booster doses was a key component to control and combat this pandemic. This study aims to monitor the heterologous and homologous booster vaccination doses that generate immune responses in healthy adults after 9 months of vaccination.

Methods

In this cross-sectional, observational study a total of 173 samples were collected. Participants from both genders (Male and Female) between the ages of 18 to 25 years were enrolled for the study. Participants who had booster shots of homologous Sinopharm BBIBP CorV and heterologous Pfizer-BioNTech vaccines were included only, with the use of a Roche Cobas-e601 analyzer, the antibody titers in the blood serum were quantified by the ECLIA method. IBM SPSS 22 was utilized for descriptive statistical analysis and P< 0.05 was considered significant.

Results

In this study the IgG antibody levels were measured against the full length of receptor binding domain (RBD) of the spike (S) protein. The mean antibody titer in the Pfizer group was 9764 ± 10976 U/mL and 5762 ± 4302 U/mL in the Sinopharm group. The Mean IgG antibody levels of the Pfizer-vaccinated group were significantly higher than the Sinopharm-vaccinated group (P=0.000, each). Comparing the Sinopharm BBIBP CorV booster dosage to the Pfizer booster, Pfizer BNT162b2demonstrated a stronger immune response. However, there were no immunological gender-specific significant differences. The administration of a third dosage of Pfizer BNT162b2 after two doses of BBIBP CorV

Conclusion

The administration of a third dosage of Pfizer BNT162b2 after two doses of BBIBP-CorV is recommended to boost the humoral immune response in the general population while there was no gender-specific difference observed. More effectiveness can be attained by administering additional doses due to the antibody decay.
A novel platelets-related gene signature for predicting prognosis, immune features and drug sensitivity in gastric cancer
Authors: Li Qun , Zhang Cheng , Ren Yulin , Qiao Lei , Xu Shuning , Li Ke , Liu Ying
Published: November 2024

Background

Platelets can dynamically regulate tumor development and progression. Nevertheless, research on the predictive value and specific roles of platelets in gastric cancer (GC) is limited. This research aims to establish a predictive platelets-related gene signature in GC with prognostic and therapeutic implications.

Methods

We downloaded the transcriptome data and clinical materials of GC patients (n=378) from The Cancer Genome Atlas (TCGA) database. Prognostic platelets-related genes screened by univariate Cox regression were included in Least Absolute Shrinkage and Selection Operator (LASSO) analysis to construct a risk model. Kaplan-Meier curves and receiver operating characteristic curves (ROCs) were performed in the TCGA cohort and three independent validation cohorts. A nomogram integrating the risk score and clinicopathological features was constructed. Functional enrichment and tumor microenvironment (TME) analyses were performed. Drug sensitivity prediction was conducted through The Cancer Therapeutics Response Portal (CTRP) database. Finally, the expression of ten signature genes was validated by quantitative real-time PCR (qRT-PCR).

Results

A ten-gene (SERPINE1, ANXA5, DGKQ, PTPN6, F5, DGKB, PCDH7, GNG11, APOA1, and TF) predictive risk model was finally constructed. Patients were categorized as high- or low-risk using median risk score as the threshold. The area under the ROC curve (AUC) values for the 1-, 2-, and 3-year overall survival (OS) in the training cohort were 0.670, 0.695, and 0.707, respectively. Survival analysis showed a better OS in low-risk patients in the training and validation cohorts. The AUCs of the nomogram for predicting 1-, 2-, and 3-year OS were 0.708, 0.763, and 0.742, respectively. TME analyses revealed a higher M2 macrophage infiltration and an immunosuppressive TME in the high-risk group. Furthermore, High-risk patients tended to be more sensitive to thalidomide, MK-0752, and BRD-K17060750.

Conclusion

The novel platelets-related genes signature we identified could be used for prognosis and treatment prediction in GC.
Autoimmune disease: a view of epigenetics and therapeutic targeting
Authors: Mu Siqi , Wang Wanrong , Liu Qiuyu , Ke Naiyu , Li Hao , Sun Feiyang , Zhang Jiali , Zhu Zhengwei
Published: November 2024

Autoimmune diseases comprise a large group of conditions characterized by a complex pathogenesis and significant heterogeneity in their clinical manifestations. Advances in sequencing technology have revealed that in addition to genetic susceptibility, various epigenetic mechanisms including DNA methylation and histone modification play critical roles in disease development. The emerging field of epigenetics has provided new perspectives on the pathogenesis and development of autoimmune diseases. Aberrant epigenetic modifications can be used as biomarkers for disease diagnosis and prognosis. Exploration of human epigenetic profiles revealed that patients with autoimmune diseases exhibit markedly altered DNA methylation profiles compared with healthy individuals. Targeted cutting-edge epigenetic therapies are emerging. For example, DNA methylation inhibitors can rectify methylation dysregulation and relieve patients. Histone deacetylase inhibitors such as vorinostat can affect chromatin accessibility and further regulate gene expression, and have been used in treating hematological malignancies. Epigenetic therapies have opened new avenues for the precise treatment of autoimmune diseases and offer new opportunities for improved therapeutic outcomes. Our review can aid in comprehensively elucidation of the mechanisms of autoimmune diseases and development of new targeted therapies that ultimately benefit patients with these conditions.
Comparison of treatment models for single primary advanced gallbladder cancer
Authors: Li Rongxuan , Chen Xiao , Wang Bingchen , Ai Bolun , Min Fangdi , Cao Dayong , Zhou Jianguo , Yan Tao
Published: November 2024

Purpose

Treatment for advanced gallbladder cancer (GBC) remains controversial, with various recommendations regarding the choice and combination of surgery and adjuvant therapy. The present article is targeting for the exploration of optimal treatment models for advanced GBC.

Methods

AJCC (American Joint Committee on Cancer, 8th edition) stage III and stage IV GBC, were defined as advanced GBC. Patients with advanced GBC were identified using the Surveillance, Epidemiology, and End Results (SEER) database and departmental cohort. Because of the most representative, only gallbladder adenocarcinoma (GBAC) patients were selected. Based on their surgical status (No, Non-radical and Radical surgery), chemotherapy status (Chemotherapy, No chemotherapy), and radiotherapy status (Radiotherapy, No radiotherapy), treatment models were categorized. For the purposes of evaluating the treatment outcomes of various treatment models and determining the risk element for cancer-specific survival (CSS), Cox regression analysis was applied. Kaplan-Meier curves were used before and after adjusting for covariates, with log-rank tests used to analyze discrepancies between curves. Immunotherapy was analyzed using clinical data from departmental cohort. Finally, to compensate for the limitations of the database, a review examines the progress in treatment models for advanced GBC.

Results

5,154 patients aged over 18 years with solitary primary advanced GBC were identified from the SEER database. In advanced GBC patients, the treatment model has emerged as a significant prognostic factor. “Radical surgery + Chemotherapy + Radiotherapy” models maximally improved the CSS of advanced GBC before and after adjusting for covariates, while “No surgery + No chemotherapy + No radiotherapy” model had the lowest CSS. The present conclusions were supported even after subgroup analysis by AJCC stage. The efficacy of immunotherapy was demonstrated in the departmental cohort analysis. Additionally, this article provides a comprehensive overview of recent advancements in various emerging treatment strategies.

Conclusion

Even when optimal treatment model cannot be pursued, providing comprehensive combinations of treatments to advanced GBC patients whenever possible is always beneficial for their survival.
Association of dementia with the 28-day mortality of sepsis: an observational and Mendelian randomization study
Authors: Lan Ying , Zhu Junchen , Pu Peng , Ni Wentao , Yang Qilin , Chen Lvlin
Published: November 2024

Background

Observational research suggests that individuals with dementia who have sepsis face a higher likelihood of death. However, whether there is a causal relationship between the two remains unknown.

Methods

We analyzed data from patients diagnosed with sepsis and dementia, extracted from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database. To examine the correlation between dementia and 28-day mortality in sepsis, we utilized Cox proportional hazards models. Following this, we performed a Mendelian randomization (MR) study with two samples to investigate the potential link between dementia and mortality within 28 days in sepsis.

Results

This study included a total of 22,189 patients diagnosed with sepsis, among whom 1,346 cases (6.1%) had dementia. After adjusting for multiple confounding factors, dementia was associated with an increased risk of 28-day mortality in sepsis (HR = 1.25, 95% CI = 1.12–1.39, p < 0.001). In the MR analysis, there appeared to be a causal relationship between genetically predicted dementia with Lewy bodies (DLB) (OR = 1.093, 95% CI = 1.016–1.177, p = 0.017) and 28-day mortality in sepsis. However, there was no evidence of causality between any dementia (OR = 1.063, 95% CI = 0.91–1.243, p = 0.437), Alzheimer’s disease (AD) (OR = 1.126, 95% CI = 0.976–1.299, p = 0.103), vascular dementia (VD) (OR = 1.008, 95% CI = 0.93–1.091, p = 0.844), and the risk of 28-day mortality in sepsis.

Conclusion

In the observational analysis, dementia was associated with an increased risk of 28-day mortality in septic patients. However, in the MR analysis, only DLB was associated with increased 28-day mortality in septic patients, with no observed correlation for other dementia subtypes.
The novel estrogen receptor beta agonist EGX358 and APOE genotype influence memory, vasomotor, and anxiety outcomes in an Alzheimer’s mouse model
Authors: Schwabe M. R. , Fleischer A. W. , Kuehn R. K. , Chaudhury S. , York J. M. , Sem D. S. , Donaldson W. A. , LaDu M. J. , Frick K. M.
Published: November 2024

Introduction

Alzheimer’s disease (AD) prevalence and severity are associated with increased age, female sex, and apolipoprotein E4 (APOE4) genotype. Although estrogen therapy (ET) effectively reduces symptoms of menopause including hot flashes and anxiety, and can reduce dementia risk, it is associated with increased risks of breast and uterine cancer due to estrogen receptor alpha (ERα)-mediated increases in cancer cell proliferation. Because ERβ activation reduces this cell proliferation, selective targeting of ERβ may provide a safer method of improving memory and reducing hot flashes in menopausal women, including those with AD. APOE genotype influences the response to ET, although it is unknown whether effects of ERβ activation vary by genotype.

Methods

Here, we tested the ability of long-term oral treatment with a novel highly selective ERβ agonist, EGX358, to enhance object recognition and spatial recognition memory, reduce drug-induced hot flashes, and influence anxiety-like behaviors in female mice expressing 5 familial AD mutations (5xFAD-Tg) and human APOE3 (E3FAD) or APOE3 and APOE4 (E3/4FAD). Mice were ovariectomized at 5 months of age and were then treated orally with vehicle (DMSO) or EGX358 (10 mg/kg/day) via hydrogel for 8 weeks. Spatial and object recognition memory were tested in object placement (OP) and object recognition (OR) tasks, respectively, and anxiety-like behaviors were tested in the open field (OF) and elevated plus maze (EPM). Hot flash-like symptoms (change in tail skin temperature) were measured following injection of the neurokinin receptor agonist senktide (0.5 mg/kg).

Results

EGX358 enhanced object recognition memory in E3FAD and E3/4FAD mice but did not affect spatial recognition memory. EGX358 also reduced senktide-induced tail temperature elevations in E3FAD, but not E3/4FAD, females. EGX358 did not influence anxiety-like behaviors or body weight.

Discussion

These data indicate that highly selective ERβ agonism can facilitate object recognition memory in both APOE3 homozygotes and APOE3/4 heterozygotes, but only reduce the magnitude of a drug-induced hot flash in APOE3 homozygotes, suggesting that APOE4 genotype may blunt the beneficial effects of ET on hot flashes. Collectively, these data suggest a potentially beneficial effect of selective ERβ agonism for memory and hot flashes in females with AD-like pathology, but that APOE genotype plays an important role in responsiveness.
Causal associations of ischemic stroke, metabolic factors, and related medications with epilepsy: a Mendelian randomization study
Authors: Wang Wencai , Liu Menghao , Liu Fengling , Wang Zun , Ye Wei , Li Xianfeng
Published: November 2024

Background

Earlier researches have demonstrated that ischemic stroke, metabolic factors, and associated medications may influence the risk of epilepsy. Nevertheless, the causality between these elements and epilepsy remains inconclusive. This study aims to examine whether ischemic stroke, metabolic factors, and related medications affect the overall risk of epilepsy.

Methods

We used single nucleotide polymorphisms associated with ischemic stroke, hypothyroidism, hypertension, blood glucose levels, high cholesterol, serum 25-Hydroxyvitamin D levels, testosterone, HMG CoA reductase inhibitors, and beta-blocking agents as instrumental variables in a Mendelian randomization technique to investigate causality with epilepsy. Multiple sensitivity methods were performed to evaluate pleiotropy and heterogeneity.

Results

The IVW analysis revealed positive associations between ischemic stroke (OR = 1.29; p = 0.020), hypothyroidism (OR = 1.05; p = 0.048), high blood pressure (OR = 1.10; p = 0.028), high cholesterol (OR = 1.10; p = 0.024), HMG CoA reductase inhibitors (OR = 1.19; p = 0.003), beta-blocking agents (OR = 1.20; p = 0.006), and the risk of epilepsy. Conversely, blood glucose levels (OR = 0.79; p = 0.009), serum 25-Hydroxyvitamin D levels (OR = 0.75; p = 0.020), and testosterone (OR = 0.62; p = 0.019) exhibited negative associations with the risk of epilepsy. Sensitivity analyses confirmed the robustness of these findings (p > 0.05).

Conclusion

Our research suggests that ischemic stroke, hypothyroidism, high blood pressure, high cholesterol, HMG CoA reductase inhibitors, and beta-blockers may increase the risk of epilepsy, whereas serum 25-Hydroxyvitamin D levels and blood glucose levels may reduce the risk.
Association between platelet-to-high-density lipoprotein cholesterol ratio and future stroke risk: a national cohort study based on CHARLS
Authors: Hou Xin , Zhu Meibao , Zhu Zhenghao , Li Yanhui , Chen Xinmin , Zhang Xiaohong
Published: November 2024

Background

According to recent research, there is a considerable correlation between the severity of coronary artery disease and the platelet-to-high-density lipoprotein cholesterol ratio (PHR), which suggests that PHR is a potentially valuable inflammatory biomarker. However, the body of current research offers insufficiently strong evidence to clarify the connection between PHR and the incidence of stroke. Therefore, this study aims to elucidate any potential associations between PHR and stroke risk.

Methods

This study employed data from the China Health and Retirement Longitudinal Study (CHARLS) covering the period from 2011 to 2018. It included 5,872 participants who did not have a history of stroke in 2011. These patients were separated into four groups according to their baseline PHR quartiles. The main goal of the study was to focus on stroke outcomes. Stroke was defined as an occurrence of a cerebrovascular accident confirmed by a physician. We employed Cox proportional hazards regression models to investigate the association between PHR and the likelihood of experiencing a stroke. Furthermore, we conducted restricted cubic spline regression analysis and subgroup analysis.

Results

The average follow-up period was 77.5 months, during which 390 participants experienced a stroke. In comparison to the lowest quartile group, participants in the highest quartile of PHR had a 49% increased risk of stroke (HR 1.49, 95% CI 1.13–1.96, p = 0.004). The adjusted multivariable Cox regression analysis maintained the statistical significance of this association (aHR 1.42, 95% CI 1.06–1.90, p = 0.019). After adjustment, a positive linear relationship between stroke risk and PHR was identified through restricted cubic spline regression analysis (nonlinear p > 0.05). Additionally, the impact of stroke was consistent across a variety of subgroups, as evidenced by subgroup analysis.

Conclusion

Our study indicates that higher PHR levels are significantly associated with an increased risk of stroke and that these levels can be used to identify groups that are at high risk of stroke.
A mobile electrical stimulator for therapeutic modulation of the vestibular system — design, safety, and functionality
Authors: Kollmansperger Sandra , Decker Julian , Berkes Sebastian , Jahn Klaus , Wuehr Max
Published: November 2024

Low-intensity noisy galvanic vestibular stimulation (nGVS) is a promising non-invasive treatment for enhancing vestibular perceptual performance and postural control in patients with chronic vestibular hypofunction. However, this approach has so far been studied mainly under laboratory conditions. Evidence indicates that continuous application of nGVS in daily life is necessary for it to be effective. To address this need, we have developed a mobile nGVS stimulator and conducted a series of pilot studies to evaluate its safety, tolerability, functionality, and therapeutic effects. The device is a lightweight, compact, and portable AC stimulator featuring a user-friendly interface for the individualized adjustment of nGVS parameters. It includes an integrated motion sensor that automatically activates stimulation during body movement and deactivates it during inactivity, optimizing its practical use in real-world settings. The stimulator adheres to strict safety standards and, in initial long-term use, has exhibited only mild side effects (e.g., skin irritation and headaches), likely attributable to the current electrode placement, which requires further optimization. As expected, the device consistently elicits known vestibular sensorimotor reflex responses in healthy individuals. Importantly, further pilot studies in healthy participants demonstrate that the device can reliably replicate known facilitating effects on vestibular perception and postural control. Together, these findings suggest that this mobile stimulation device can facilitate the translation of nGVS into therapeutic everyday use.
Impact of sports vision training on visuomotor skills and shooting performance in elite skeet shooters
Authors: Guo Yuqiang , Yuan Tinggang , Peng Jian , Deng Liwei , Chen Chao
Published: November 2024

Introduction

Vision serves as a critical channel for athletes to acquire information during competitions and constitutes a vital component of their competitive ability. Through scientifically designed sports visual training, specific visual skills can be enhanced, thereby assisting athletes in achieving optimal performance in competitive settings. This study aim to explore the visuomotor abilities and shooting performance of skeet shooters through Sports Vision Training (SVT).

Methods

Twenty elite skeet shooters were recruited and randomly assigned to an experimental group (EXP, n = 10) and a control group (CON, n = 10). The EXP underwent 6-week of SVT on Senaptec Seneory Station, twice a week, while the CON completed an equivalent workload of target-tracking training.

Result

Visuomotor skills testing showed significant improvements in Near- Far Quickness, Perception Span, and Eye-hand Coordination in the EXP (p < 0.05), with no changes in the CON. Comparative post-test results between the two groups showed significant differences in N/F Q, Target Capture, Perception Span, Eye-hand Coordination, and Go/ No Go (p < 0.05). In shooting performance indicators, EXP shooters showed a highly significant improvement in hit accuracy (p < 0.01), with a similar difference compared to the CON. Additionally, they exhibited a highly significant improvement in shotgun-mounting reaction time (p < 0.01). Kinematic indicators of shotgun movement during the firing process for shot 2 showed significant differences in peak velocity (p < 0.01), X-axis (p = 0.033) and Y-axis (p = 0.001) displacement.

Conclusion

SVT can enhance visuomotor abilities in skeet shooters and has a positive impact on their shooting technique. This is primarily manifested in shorter shotgun-mounting reaction time and improved efficiency in action at shot 2, effectively improving their shooting accuracy.
Eye movement characteristics of emotional face recognizing task in patients with mild to moderate depression
Authors: Yang Qian , Fu Yanyan , Yang Qiuli , Yin Dongqing , Zhao Yanan , Wang Hao , Zhang Han , Sun Yanran , Xie Xinyi , Du Jian
Published: November 2024

Objective

Depression is a complex affective disorder characterized by high prevalence and severe impact, commonly presenting with cognitive impairment. The objective diagnosis of depression lacks precise standards. This study investigates eye movement characteristics during emotional face recognition task (EFRT) in depressive patients to provide empirical support for objective diagnosis.

Methods

We recruited 43 patients with depression (Depressive patients, DP) from a psychiatric hospital and 44 healthy participants (Healthy Control, HC) online. All participants completed an EFRT comprising 120 trials. Each trial presented a gray screen for 800 ms followed by a stimulus image for judgment. Emotions were categorized as positive, neutral, or negative. Eye movement trajectories were recorded throughout the task. Latency of First Fixation (LFF), Latency of First Fixation for Eye AOI, and Latency of First Fixation for Mouth AOI were used as representative indicators of early attention, Proportion of Eye AOI, and Proportion of Mouth AOI as measures of intermediate attention, Accuracy (ACC) and Reaction Time (RT) as behavioral indicators of late-stage attention. In this study, these metrics were employed to explore the differences between patients with depression and healthy individuals.

Results

Compared to healthy participants, individuals with depression exhibit longer first fixation latencies on the eyes and mouth during the early attention stage of emotional face recognition, indicating an avoidance tendency toward key facial recognition cues. In the mid-to-late attention stages, depressive individuals show an increased fixation ratio on the eyes and a decreased fixation ratio on the mouth, along with lower accuracy and longer response times. These findings suggest that, relative to healthy individuals, individuals with depression have deficits in facial recognition.

Conclusion

This study identified distinct attention patterns and cognitive deficits in emotional face recognition among individuals with depression compared to healthy individuals, providing an attention-based approach for exploring potential clinical diagnostic markers for depression.
Consideration of the role of protein quality in determining dietary protein recommendations
Authors: Wolfe Robert R. , Church David D. , Ferrando Arny A. , Moughan Paul J.
Published: November 2024

The quality of a dietary protein refers to its ability to provide the EAAs necessary to meet dietary requirements. There are 9 dietary amino acids that cannot be metabolically produced in the body and therefore must be consumed as part of the diet to avoid adverse metabolic consequences. These essential amino acids (EAAs) serve a variety of roles in the body. The amount and profile of the dietary EAAs relative to the individual EAA requirements and the digestibility of the dietary protein are the key factors that determine its quality. Currently the Digestible Indispensable Amino Acid Score (DIAAS) is the best available approach to quantifying protein quality. The most prominent metabolic role of dietary EAAs is to stimulate protein synthesis by serving as signals to activate molecular mechanisms responsible for the initiation of protein synthesis and, most importantly, to provide the necessary precursors for the synthesis of complete proteins. Current dietary recommendations generally do not consider protein quality. Accounting for protein quality in dietary patterns can be accomplished while staying within established ranges for dietary protein consumption. Poor protein quality can be compensated for to some extent by eating more low-quality protein, but to be effective (“complementary”) the limiting EAA must differ between the low-quality protein and the base diet to which it is being supplemented. Adding a high-quality protein to a dietary pattern based on low-quality protein is more effective in meeting EAA goals than increasing the amount of low-quality protein, even if the low-quality proteins are complementary. Further, reliance entirely on low-quality protein food sources, particularly in circumstances that may benefit from a level of dietary EAAs greater than minimal requirements, is likely to include excessive caloric consumption. While protein consumption in high-income nations is generally perceived to be adequate or even excessive, assessment of dietary patterns indicates that a significant percentage of individuals may fall short of meeting optimal levels of EAA consumption, especially in circumstances such as aging in which the optimal EAA consumption is greater than basal values for healthy young individuals. The case is made that protein quality is an important consideration in meeting EAA requirements.
From semi-starvation to the stage: a case report on indicators of low energy availability in a drug-free bodybuilder during contest preparation and peak week
Authors: Ritson Alex J. , McDonald Lyle , Agu Joseph , Bannock Laurent G.
Published: November 2024

Natural bodybuilding competitions involve periods of low energy availability (EA) combined with resistance training and high-protein diets to achieve extreme leanness. This study tracked a drug-free bodybuilder adopting evidence-based nutrition practices during 18 weeks of contest preparation. We measured endocrine function, resting energy expenditure, respiratory exchange ratio, body composition, resting heart rate, oral temperature, mood, and strength performance. Endocrine function was remeasured after 2 days of energy repletion. From baseline to week 18, free triiodothyronine (T3) and total testosterone (TT) fell into clinically low (2.7 pmol/L−1) and sub-clinically low (9.1 nmol/L−1) ranges. Resting energy expenditure decreased by −519 kcal (REEratio 0.78), and respiratory exchange ratio decreased from 0.95 to 0.85. Body mass reduced by −5.1 kg, with a sum of eight skinfold loss of −15.7 mm. Correlations were observed between body mass and decreases in oral temperature (r = 0.674, p = 0.002) and resting heart rate (r = 0.560, p = 0.016). Mood remained stable until the final 2 weeks and relative one-repetition maximum decreased in the squat (−5.4%), bench (−2.6%), and deadlift (−3.6%). Following 2 days of modest energy repletion, free T3 increased (18.5%), returning to sub-clinically low values (3.2 pmol/L−1), whereas TT fell (−20.9%), reaching clinically low values (7.2 nmol/L−1). These results offer insight into the dynamics of T3 and TT following a short-term period of modest energy repletion and further information on indicators of low EA during chronic energy restriction.
Extraction, purification, structural characterization, bioactivities, modifications and structure–activity relationship of polysaccharides from Ophiopogon japonicus: a review
Authors: Zhang Yiqiang , Chen Bo , Zhang Hua , Zhang Juan , Xue Juan
Published: November 2024

Ophiopogon japonicus (Thunb.) Ker Gawl., is a traditional Chinese medicine (TCM) with a history of thousands of years. O. japonicus polysaccharides (OJPs), as one of the main active ingredient, are a kind of representative pharmacological bioactive macromolecules and mainly composed of Glc and Fru with molecular weight between 2.48 and 325 kDa. OJPs have a variety of biological activities, such as hypoglycemic, cardioprotective, immunomodulatory, improvement of obesity, and renal protective activity. In this review, the extraction, purification and structural characterization of OJPs were systematically reviewed, and its biological function, molecular mechanism and structure–activity relationship were analyzed. Additionally, this review summarized and found that structural modifications such as liposome modification, sulfation modification, and polyethylene glycol modification have improved the pharmacological activity and bioavailability of OJPs. Overall, this work will help to better understand OJPs and provide a theoretical review of their further development and utilization in the field of medicine and health food.
When multiple primary lung cancers express the same rare mutation: a case report
Authors: Han Yaqing , Geng Yandong , Sui Qian , Liu Yanjie , Xie Shaonan , Gao Maogang , Liu Qingyi , Liu Guangjie , Wang Shize
Published: November 2024

The debate continues whether the expression of the same rare genetic mutation in multiple primary lung cancers suggests intrapulmonary metastasis or truly multiple primary lung cancers. We report a case of a 54-year-old female patient who presented with multiple nodules in the right lung discovered during a routine examination, persisting for six months. The patient had three central lesions in the right lung’s upper, middle, and lower lobes. She underwent thoracoscopic wedge resection, and the postoperative pathology reported two minimally invasive adenocarcinoma and one adenocarcinoma in situ. Interestingly, genetic testing for lung cancer-related driver genes revealed the presence of the rare RET mutation in all three nodules. This led us to speculate that these nodules might have the exact origin rather than being multiple primaries. To verify this hypothesis, we conducted further testing on these nodules, including whole-exome sequencing (The NGS data was generated from the Illumina sequencing platform by Novogene Co. Ltd, Beijing, China). The results indicated that although all three nodules expressed the RET mutation, there was significant heterogeneity in the gene mutations (differences in the number of cellular mutations, substitution composition levels, and clustering analysis of the three nodules). Thus, the patient was considered to have multiple primary lung cancers. In such cases, whole-exome sequencing can distinguish whether the nodules have the exact origin.
Identification of key programmed cell death genes for predicting prognosis and treatment sensitivity in colorectal cancer
Authors: Ma Jian-ying , Wang Yi-xian , Zhao Zhen-yu , Xiong Zhen-yu , Zhang Zi-long , Cai Jun , Guo Jia-wei
Published: November 2024

Colorectal cancer (CRC) ranks third in global incidence and second in mortality. However, a comprehensive predictive model for CRC prognosis, immunotherapy response, and drug sensitivity is still lacking. Various types of programmed cell death (PCD) are crucial for cancer occurrence, progression, and treatment, indicating their potential as valuable predictors. Fourteen PCD genes were collected and subjected to dimensionality reduction using regression methods to identify key hub genes. Predictive models were constructed and validated based on bulk transcriptomes and single-cell transcriptomes. Furthermore, the tumor microenvironment, immunotherapy response, and drug sensitivity profiles among patients with CRC were explored and stratified by risk. A risk score incorporating the PCD genes FABP4, AQP8, and NAT1 was developed and validated across four independent datasets. Patients with CRC who had a high-risk score exhibited a poorer prognosis. Unsupervised clustering algorithms were used to identify two molecular subtypes of CRC with distinct features. The risk score was combined with the clinical features to create a nomogram model with superior predictive performance. Additionally, patients with high-risk scores exhibited decreased immune cell infiltration, higher stromal scores, and reduced responsiveness to immunotherapy and first-line clinical drugs compared with low-risk patients. Furthermore, the top ten non-clinical first-line drugs for treating CRC were selected based on their predicted IC50 values. Our results indicate the efficacy of the model and its potential value in predicting prognosis, response to immunotherapy, and sensitivity to different drugs in patients with CRC.
Low-dose trimethoprim-sulfamethoxazole treatment for Pneumocystis pneumonia: a systematic review and meta-analysis
Authors: Huang Hui-Bin , Zhu Yi-Bing , Yu Da-Xing
Published: November 2024

Background

The recommended standard treatment for Pneumocystis jirovecii pneumonia (PJP) is high-dose trimethoprim-sulfamethoxazole (TMP-SMX) (15–20 mg/kg/d TMP). However, the standard regimen may cause a high incidence of dose-related adverse events (AEs). Therefore, we aimed to conduct a systematic review and meta-analysis to evaluate the efficacy and safety of low-dose TMP-SMX regimens (<15 mg/kg/d of TMP) compared with the standard regimen in patients with PJP.

Methods

We searched PubMed, Embase, and the Cochrane database for relevant articles from inception to 10 March 2024. Studies were included if they focused on PJP patients receiving a low-dose TMP-SMX regimen compared with a standard regimen. The primary outcome was mortality. We assessed study quality and performed subgroup analysis and sensitivity analysis to explore potential heterogeneity among the included studies.

Results

Seven studies were included. Overall, the low-dose regimen significantly reduced the risk of mortality (odds ratio [OR] = 0.49; 95% CI, 0.30–0.80; I2 = 16%; P = 004). This finding was confirmed in further sensitivity and subgroup analyses. The low-dose regimen also significantly reduced total AEs (OR = 0.43; 95% CI, 0.29–0.62; I2 = 0%; P < 0.0001), and improved the incidence of most specific AEs (ORs ranged from 0.13 to 0.89). In addition, the low-dose regimen had significantly more patients completing the initial regimen (P = 0.002), fewer patients requiring dose reductions (P = 0.04), and almost significantly fewer patients requiring a switch to a second-line regimen (P = 0.06).

Conclusion

The limited available evidence suggests that a low-dose TMP-SMX regimen significantly reduced mortality and total AEs in PJP patients. Thus, it is one of the potentially promising therapies to PJP and more high-quality and multi-center randomized trials should be conducted in the future.
Risks of malignancies related to disease-modifying antirheumatic drugs in rheumatoid arthritis: a pharmacovigilance analysis using the FAERS database
Authors: Xiong Wan , Li Yilin , Hu Lin , He Gefei , Huang Juanjuan
Published: November 2024

Objectives

Over the years when disease-modifying antirheumatic drugs (DMARDs) have been used in rheumatoid arthritis patients, reports of malignancies have emerged. This study aims to investigate the association between malignancies and DMARDs by using data extracted from the Food and Drug Administration Adverse Event Reporting System (FAERS).

Methods

FAERS data (January 2019 to December 2023) were reviewed. For each drug-event pair, the disproportionality analysis was conducted to evaluate the risk of malignancy. Multivariate logistic regression was implemented to mitigate potential biases. Moreover, the time to onset of malignancy was also evaluated.

Results

We conducted a detailed search for rheumatoid arthritis indications and identified a total of 17,412 adverse event reports associated with malignancies, with selective DMARDs designated as the role code “primary suspect”. At the preferred term level, there were 198 positive signals, among which the lower limit of the 95% confidence interval for the information component is 3.55 for squamous cell carcinoma of the skin, 2.39 for breast cancer, and 2.27 for lymphoproliferative disorder. In comparison to other DMARDs, targeted synthetic DMARDs were associated with a broader range of malignancies at both preferred term and Standardized MedDRA Queries levels. The number of adverse events reported in female patients is approximately 2–3 times higher than men, and the median age across the population was approximately 62 years. In terms of onset time, the conventional synthetic DMRADs exhibited a relatively longer median time, ranging from 3.58 to 7.08 years, while the targeted synthetic DMARDs demonstrated a shorter median time of 0.83–1.67 years.

Conclusion

Our study uncovers varying degrees of malignancy risks related to DMARDs, with a significantly higher risk observed in targeted synthetic DMARDs. Additionally, novel malignancy signals, not documented in product labels, have been detected. In the future, further research will be necessary to validate our findings.