bluuebunny/crossref_metadata_2025 huggingface dataset:
CIMETID1NE ENHIBITS ELIMINAT1ON OF SULPHAMETHOXAZOLE IN RABBIT {Oryctolagus Cuniculus)
Authors: Tjahajati Ida
Published: November 2022

Cimetidine was reported to inhibit elimination many other drugs. The research has been done to study the effect of cimetidine on elimination of sulphamethoxazole in rabbits. Twenty healthy male rabbits, 10 months of age were used as experimental animals. The rabbits were allotted into 4 groups of 5 each Group I as a control group were intramuscularly injected with 25 mg sulphamethoxazole. Group 11 were intramuscularly injected 25 sutphamethoxazole and pretreated with 12.5 mg cimetidine 30 minutes before injection. Group 111 were intramuscularly injected 25 mg sulphamethoxazole, pretreatod with 25 mg cimetidine 30 minutes before injection. Group IV were intramuscularly injected 25 mg sulphamethoxazole, pretreated with 50 mg cimetidine 30 minutes before injection. Blood sample were collecled at 0.25, 0 75, 1,75, .1.75, 10 and 24 hours after injection. Plasma sulphamethoxazole levels were determined using the Bratton-Marshall method Elimination kinetic parameters were analyzed statistically using anava. The result of the study showed that cimetidine significantly reduced total clearance of sulphamethoxazole <P<0.l). Based on the result it can be concluded that cimetidine inhibits elimination of sulphamethoxazole.
THE EFFECT OF HIGH FAT DIET ON THE CONCENTRATION RATIO BETWEEN PROOXIDANT AND TOTAL ANTIOXIDANT IN RAT (Sprague dawley)
Authors: Mulyani Guntari Titik , Wuryastuti Hastari
Published: November 2022

The objective of the investigation was to study the relationship between ratio prooxidant and total antioxidant (peroxidation index) and cell damage in male rats. Fourty male Sprague dawley rats, 3 month of age, 150-200 g of body weight were alloted randomly to 2 groups of 20 each. The first group was fed normal diet (control), the second group was fed high fat diet (20% fat). After 2 weeks, the blood samples were taken out from 5 rats of each group for measur­ing prooxidants and antioxidants. The prooxidants and anti-oxidants st-atus in this study was done by measuring thio barbituric add subs tance I TEARS) and total antloxldant status (TAS) concentration. The heart, liver and kidney tissues were collected for histopatological analysis, Perox­idation index (PI) were determined by dividing TBARS value with TAS value. The same procedures were repeated after 4, 8, and 16 weeks. Analysis of the data showed that after 16 weeks on experimental diet, the mean concentration of TBARS in blood of group I was 1.45Q+G.158 umol/L and the mean concentration of TBASS in blood of group 11 was 4,75 + 2,17 nmol/L. The highest ratio between prooxidant and total antioxidant was 17.47 and the Lowest ratio between oxidant and total antioxidant was 0.849. Analysis of histopatology showed that there were hyalinitation and oardiomyopathy in the heart tissu, fatty liver degeneration and necrosis in the liver and necrose and atrophy of the kidney. From the results can be concluded that (1) feeding high fat diet induced high ratio between proojiidant and antioxidant con^ centration (peroxidation Index). (2) Cell damage can be caused by high peroxidation index. (3) Individual variation might be caused by genetic factor.
ISOLATION, IDENTIFICATION AND ANALYSIS OF SHORT INTERSPERSED NUCLEAR ELEMENT (SINE) FROM VARANUS KOMODOENSIS
Authors: Asmara Widya
Published: November 2022

All vertebrata genomes studied so far contain SINEs, that consti tute up to 5% of the total genome. Most of the SINE sequences studied so far are mammalian genomes. There Is very limited information about SINE sequences from rep­tiles, especially of Varanus family.Isolation of SINE sequences were carried out by con­structing the komodo genomic library in E. coli. Suspected positive clones were detected by genome to genome hybridiza­tion. The SINE sequences identification were carried out by enzymatic method, and the DNA sequences were analysed with the GENETYX-SV program-Up to now there are 2 SINE sequences from komodo genome have been isolated and identified. Their sequences homo logy is 85-2% From Database search analysis with the GenBank indicated that those SlNEs were not evolved from any spe­cific tRNA gene.
THE EFFECT OF SEVERAL DESINFECTANTS ON THE SPORULATION OF THE ERIMERIA TENELLA OOCYST
Authors: Rohayati Eryl Sri , Darjono Julianti , Nurcahyo Wisnu
Published: November 2022

A research has been conducted to evaluate the effect of certain desinfectants on the speculation of the Eimerla tenclla oocysts.In this research in vitro methode has been used. This research used oocysts Eimeria tenella which have not yet Sporulated. 6 kinds of disinfectants and water as control. These desinfectants were used in the research namely : Lysol, Creolin. Kalium Jodida (KJ). Formalin, Bromoquod and Ca (OH)2.The oocysts which have not yet sporulation were imerted in these desinfectants. The duration of liners ion at each treatment was succescively 10 minutes. 20 minutes and 30 minutes. The desinfectants concentration used is of three degree of strength, in the following proportion = 1:2:4.The data colected was the percentage of oocystys which have sporulated 24 hours, 48 hours and 72 hours, after treatment. The data was analyzed with the Split Plot method (Gill. 1978).The result of research showed that each desinfectant had a different effect on the percentage of the oocyst sporulation (P < 0,01) . Bromoquod was found to be the best desinfectant, and the others were in this following order = Creolin. KJ, Water, Lysol. CafQHJn and formalin. The Concen­tration was also found significantly effective (P < 0,6) in suppressing the sporulation of the oocysts. Creolin concen­tration of the 4tri strength was found to be the best desin­fectant and the others were in this following order : water. KJ. Bromoquod, Lysol, Ca(OH)£ and formalin. The duration of imersion had no significant influence
HISTOLOGIC PICTURE OF BEEF DIGESTED BY PAYA PAPAIN ENZYME
Authors: Drastini Yatri
Published: November 2022

The study was conducted to show the histologic picture of beed digested by different concentration of PAYA papain enzyme. Twenty pieces of beed of m. femoralis, 1 x 0.5 x 0.5 cm in size, were allocated into four groups. Group I was injected with aquadest as control; group II, III, dan IV were injected with PAYA 0.25%, 0.50%, and 0.75% of the weight of meat, respectively. The samples were heated in a waterbath 65°C to activate the enzyme for 0, 15, 30, 45, and 60 minutes. Samples were then put into 10% formalin solution to stop the enzyme. The were stained with haematoxyline-eosine (HE) for histologic picture. The result shows PAYA papain enzyme starts to disintegrate myofibril in the con­centration of 0.50% for 30 minutes. The higher is the con­centration, the more do changes of myofibril take place in the form of inflamation, disintegration, and lysis.
DETECTION PROTECTIVE ANTIGEN WITH MONOCLONAL ANTIBODY OF PASTEURELLA MULTOCIDA DOMPU ISOLATE
Authors: Murwani Sri , Asmara Widya
Published: November 2022

Monoclonal antibodies can be used as specific probes for molecular structure such as drugs, hormones, receptor for hormones, or any other biologycally derived or biology-cally active materials. The potential usefulness of mono­clonal antibodies is in almost every field of biology and medicine.The study was carried out to produced monoclonal anti­bodies against outer membrane protein (OMP) Pasteurella multocida isolat Dompu, which can be used to isolate imunogenic OMP fractions.Three monoclonal antibodies which specifically react with OMP fraction of Pasteurella multocida isolat Dompu were obtained previously. Those monoclonal antibodies were pro­duced in Balb/c mouse. The antibodies production were measured by ELISA and were purified by affinity chromatograPhy.The monoclonal antibody were injected intraperitoneally into group of five rats. Two additional groups of five rats were injected with immune mouse serum and normal mouse serum, which served as a positive and negative controls respectively. All rats were challenged with 1 ml lethal dose of Pasteurella multocida 24 hours after the antibody injec­tion.The outer membrane protein was separated 12,5% SDS-PAGE. The region of gel containing the appropiate antigens (PI, P2, P3) were excised. Protein antigen was mixed with incomplete Freund's adjuvant (IFA) and injected intramuscu-lary into group of five rats at dose 0,2 ml per mouse. Two additional groups were injected with gel in IFA and OMP and IFA as a positive and negative controls. Each treated mouse and control was immunized four times, at weekly interval. Two weeks after final immunization rat were bled and their antibodies productions were measured with ELISA. All mice were then challenged with lethal dose of Pasteurella multo­cida.The result indicated that eventhough immungenic the PI, P2, P3 OMP fraction individually were not able to induce protective antibody.
EFFECTS OF SILVER INDUSTRY WASTE WATER IN KOTAGEDE, YOGYAKARTA ON HAEMTOLOGlCAL AND HISTOPATHOLOGICAL CHANGES IN EXPERIMENTAL RATS (RATTUS NORVEGICUS)
Authors: Hariono Bambang
Published: November 2022

Thirty male rats, 2,5 months old with average body weight of 92 grams were used as experimental animals.The experiment rats were adapted before for one week. The animal grouping was basedon uniformity of body weight gain, Thecontrol group consist of 15 animals and 15 animals for treated group. The treated group was given silver industry waste water from Kotagede, Yogyakana orally ad libitum the control group was supplied drinking tap water ad libitum. Blood samples via plexus retroorhitalis vein at medial cantus of the eye were taken for routine heamiolafiical examinations, clinical symptoms and body weight gain were also recorded. After treatment, for about 12 weeks, all experimental animals were sacrificed, necropsied, then the samples of brain, spinal cord, lung, tesiis. liver, and kidney were taken for histopathological preparations. The concentration of metals (especially Ag and Cu) in the liver, silver industry waste water, well water, Tap water, and soil samples (which [he silver industry waste water was discarded) were analyzed. The results of the experiment indicated thai the silver industry waste water administration in long period caused decreasing of the rat growth rate with clinical symptoms e.g. thinness,dullnessof the fur, anaemic, depression and nerve symptoms including foot paralysis. Statistical calculation showed there were not significantly changes on hematological profile between the control and treated groups, it could be due to severe dehydratation level occured, so there were reducing of blood volume. This finding was similar to leucocyte and differential counts, but there tended to decrease at week-12. There could be due 10 bone marrow depression which were caused by heavy metal intoxication. Microscopical examinaton showed there were thickness of alveoli septa, congestion, enlargement of sinusoids and infiltration of mononuclear cells around the liver centralvein, degeneration and necrosis of tubule cells, enlargement of tubule lumen and Bowman capsule space, infiltration of monoclear cells and haemorrhage performance. There were a few Purkinje cells showed more denses and the sTain more absorpted intensively (the same findings occuredin the nerve cells of spinal cord), it could be possible that there were cell structural changes, degeneration and necrosis of The nerve cells especially in the spinal cord tissues. The silver, copper and chrome concentrations in the silver industry waste water and soils samples were too high, it could be as potential sources for environmental pollution. The concentration of these metals in the tap water and well water were not detected yet.
ADHESION AND PHAGOCYTOSIS PROPERTIES OFSTREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS ISOLATES FROM INDONESIA
Authors: Salasia Siti Isrina Oktavia
Published: November 2022

An infectious diseases outbreak affecting pigs and monkeys in Bali in 1994, with case fatality rate (CFR) 75%. The disease widespread rapidly to the other district in Indonesia and developed serious problems. The causative agent involving the disease was Streptococcus equi subsp. zooepidemicus, a β-hemoIytic type of the streptococcus. The disease was consistently associated with meningitis, therefore the disease was named Streprococcal meningitis. The main aim of this study is todetect factors promoting the pathogenicity of the agent that could be showed by its characteristics and properties of the adhesions and phagocytosis. Eight and two isolates of Strepiacoccus equi subsp. zooepidemicus originated, from pigs and monkeys respectively were used in this study. The nature of bacterial surface proteins was characterized by hexadecane adherence test, hemaglutinating erythrocytes of various animals, adherence to epithelial cells and phagocytosis of the bacteria by polymorphonuclear leucocytes. The result of hexadecane adherence test showed that of the ten isolates oniy one culture of pigs isolates had a hydrophobic surface and the others were hedrophilic surface. The culture with hydrophobic surface agglutinated erythrocytes from chicken, rat and rabbit. This culture adhered in high numbers to epithelial cells and were more phagocytosed by polymorphonulear leucocytes. The cultures with hy drop hi lie surface showed no comparable reaction.
THE EFFECT OF FEVER ON ACETYLATION RATIO AND SULFADIAZINE ELEMINATION KINETICS OF LOCAL SHEEP (Ovis aries)
Authors: Tjahajati Ida , Prodjoharjono Subronto , Santoso Budiono
Published: November 2022

A Study has been conducted to identify effect of fever towards acetylafion ratio and eleminatkm kinetics of sulfadiazine in sheep. The siudy consist 10 healthy sheep using cross­over design. Each animal was treated twice with 40 days interval. In the first treatment the sheep were given 2 gram of sulfadiazine and 0.4 gram pyrimethamine. In the second treatment the animals were treated fever induction prior to treatment using 2 gram of sulfadiazin and 0.4 gram pyrimethamine. Blood samples were taken From jugular vein in hour of 0, 0.08.0.25, 0.5, 1,2,4,6,9.12, 18, and 24. Plasma sulfadiazine levels were analyzed using Bratton-Marshall Methode. Sulfadiazin parameter was calculated using Stripe program. Theresults of the study revealed that fever did not significantly (P>0,05) increased the aceiylation ratio and inhibited elemination of sulfadiazine.
A STUDY OF PREPATENT PERIODES, DEATH TIMES AND REDUCTION OF BODY WEIGHT IN EXPERIMENTAL RATS (RATTUS NORVEGICUS) INFECTED WITH TRYPANOSOMA EVANSI
Authors: Sumartono Sumartono , Rohayati Eryl Sri
Published: November 2022

The prepatent periods of T. evansi, death times and reduction of foody weights of experimental rats (Rattus norvegicus) infected with T. evansi were studied.Twelve rats were divided into four groups (group 1-!V), each group contains of three rats. The rate of the group II, group III and group IV, were infected intraperitonealy with 300, 600 and 2400 T. evansi per rat, respectively. The group I was used as a control group. After infection, their body weight were measured, followed by blood examination and detection of death time everyday.The data were collected then analysed descriptively using regression-correlation between total number of T. evansi wbich were infected, prepatent period, death time and body weight changes.The results of the experiment indicated that the higher dose of T. evansi infected, caused shorter prepatent period and death time, but there were a negative correlation between total number of T. evansi infected and percentage of body weight reduction. The evidence also showed even thought the prepatent period was shorter, all experimental rats died within 5-6 days after T. evansi detected in the peripheral blood.
Effects of homogenizing annealing on the reduction of crack formation in forgings of steel grade 08Kh18N10T
Authors: Olenin M. I. , Romanov O. N. , Kashtanov A. D. , Afanasiev S. Yu. , Berezhko B. I. , Bushuev S. V. , Shakhkyan S. A. , Apinov Zh. E. , Zernov E. A.
Published: November 2022

Experimental data on crack formation of forgings made of austenitic steel grade 08Kh18N10T are presented. It has been established that the process of crack formation is determined by the process of homogenizing annealing, which is carried out during heating of ingots and forgings before the first and second removals. The development of a technology for forging large ingots made of austenitic steel grade 08Kh18N10T, taking into account these results, leads to an increase in the quality of forging and makes it possible to achieve forging without cracking.
Strengthening of A Ni–W coating by laser processing
Authors: Krasikov A. V. , Markov M. A. , Merkulova M. V. , Staritsyn M. V.
Published: November 2022

Calorimetric studies show that the nanocrystalline coating Ni–37 wt. %W produced by electrodeposition does not undergo phase transformations up to a temperature of 1000°C. Laser processing affects the properties of coatings, and in the selected mode leads to a significant grain growth in the coating, without affecting its chemical composition. At a certain energy input, a notable increase in the microhardness of the coating was revealed.
Corrosion resistance of composite NI-P coatings in various aggressive media
Authors: Polukhin D. S. , Goikhenberg Yu. N. , Bodrov E. G.
Published: November 2022

The paper studies corrosion resistance in highly aggressive media of composite nickel-phosphorus coatings after isothermal annealing at different temperatures accompanied by crystallization. The phase composition of chemically deposited Ni-P coating containing about 1% dispersed SiC was analyzed. Gravimetric method was used to determine the mass loss of the samples as a result of daily exposure to various acids and in a solution of nitric acid with a concentration from 5 to 65%, which is extremely aggressive for Ni-P coatings. At each heat treatment, the steel witness samples were used to determine the microhardness by the Vickers method at a load of 100 g. The dependence of the parameters of the corrosion process on the presence of a dispersed additive and the phase composition of the coating has been established. At low holding times the dispersed phase exhibits a barrier effect reducing crystalline phosphide Ni3P formation during annealing and corrosion resistance; meanwhile, prolonged holding at lower temperatures produces about 70% Ni3P, stable high hardness values and improved corrosion resistance values. Lowering the coating heat treatment temperature in an oxidizing environment reduces the intensity of phosphorus burn-off from the surface and decreases all coating properties. The concentration of nitric acid in the solution at the level of 5-15% is critical and contributes to the dissolution of all coatings, regardless of their composition.

The conducted research and revealed regularities made it possible to determine the contribution of the phase composition and presence of the dispersed additive to the formation of the main service characteristics of the nickel-phosphorus coatings - microhardness and resistance to aggressive media, as well as to determine the technological modes of heat treatment that allow the formation of optimum properties of products used in the oil and gas industry.
Полипропиленовые нити, модифицированные марганецсодержащими наночастицами
Authors:   ФГБУН ФИЦ химической физики им. Н. Н. Семенова Российской академии наук, Юрков Г. Ю. , Пророкова Н. П. , Козинкин А. В. , Вавилова С. Ю. , Солодилов В. И. , Максимова А. В. , Власенко В. Г. , Кириллов В. Е. , Бузник В. М. ,   ФГБОУ ВО “Московский государственный технический университет имени Н. Э. Баумана (национальный исследовательский университет)”,   ФГБУН Институт химии растворов им. Г. А. Крестова Российской академии наук,   ФГБОУ ВО “Ивановский государственный политехнический университет”,   Научно-исследовательский институт физики ФГАОУ ВО “Южный федеральный университет”
Published: November 2022

Определено влияние малого количества полиэтилена низкой плотности (ПЭНП), полиэтилена высокой плотности и инкапсулированных наночастиц оксида марганца на механические свойства и структуру полипропиленовых моноволокон. Показано, что введение в полипропилен малого количества ПЭНП способствует повышению степени кристалличности полимерной смеси и уменьшению размера кристаллитов. Продемонстрировано, что прочность и равномерность структуры моноволокон, сформованных из смеси полиолефинов, не ухудшаются. Разработана технология создания комплексных нитей на основе полипропилена с введенным в него композитным материалом на основе ПЭНП и марганецсодержащих наночастиц. Проведены комплексные исследования структуры и свойств марганецсодержащих наночастиц, локализованных в объеме ПЭНП. Установлено, что полипропиленовые нити, наполненные марганецсодержащими нанокомпозитами на основе ПЭНП, обладают улучшенными характеристиками: возрастает прочность при растяжении, снижается поверхностное электрическое сопротивление, появляется способность подавлять жизнедеятельность грамположительных патогенных бактерий и микроскопических грибков.
Влияние начального напряженного состояния на эффективные свойства пьезокомпозита
Authors:   Пермский национальный исследовательский политехнический университет, Паньков А. А.
Published: November 2022

Получено новое численно­аналитическое решение задачи “эффективного модуля” статистической механики пьезокомпозитов с учетом наличия начального напряженного электромагнитоупругого состояния нерегулярной структуры с эллипсоидальными неоднородностями. Использован метод функций Грина для однородной пьезоэлектромагнитоупругой среды. Новые решения подтверждены сравнением с известным асимптотическим решением для случая упругого слоистого композита при наличии начального напряженного состояния слоев. Представлены результаты численного анализа влияния начального напряженного состояния на эффективные свойства упругих двухфазных композитов со слоистой, однонаправленно­волокнистой и гранулированной структурами. Для композита со сферическими включениями выявлено появление на макроуровне “наведенной” анизотропии, обусловленной наличием начального напряженного состояния структуры. Дан численный анализ влияния осесимметричного макроуровневого начального напряженного состояния на эффективные трансверсально­изотропные электроупругие свойства композита PZT­4/фторопласт с однонаправленными пьезоэлектрическими волокнами. Выявлены эффективные характеристики пьезокомпозита, на которые начальное напряженное состояние оказывает существенное влияние.
Non-native hosts of an invasive seaweed holobiont have more stable microbial communities compared to native hosts in response to thermal stress
Authors: Bonthond Guido , Neu Anna-Katrin , Bayer Till , Krueger-Hadfield Stacy , Künzel Sven , Weinberger Florian
Published: November 2022

Seaweeds are colonized by a microbial community which can be directly
linked to their performance. This community is shaped by an interplay of
stochastic and deterministic processes, including mechanisms which the
holobiont host deploys to manipulate its associated microbiota. The Anna
Karenina Principle predicts that when a holobiont is exposed to
suboptimal or stressful conditions, these host mechanisms may be
compromised. This leads to a relative increase of stochastic processes
that may potentially result in the succession of a microbial community
harmful to the host. Based on this principle, we used the variability in
microbial communities (i.e., beta diversity) as a proxy for stability
within the invasive holobiont Gracilaria vermiculophylla during a
simulated invasion in a common garden experiment. At elevated
temperature (22 °C), host performance declined and disease incidence and
beta diversity increased. At optimal temperature (15 °C), beta diversity
did not differ between native and non-native populations. However, under
thermally stressful conditions beta diversity increased more in epibiota
from native populations. This suggests that epibiota associated with
holobionts from non-native populations are under thermal stress more
stable than holobionts from native populations. This pattern reflects an
increase of deterministic processes acting on epibiota associated with
non-native hosts, which in the setting of a common garden can be assumed
to originate from the host itself. Therefore, these experimental data
suggest that the invasion process may have selected for hosts better
able to maintain stable microbiota during stress. Future studies are
needed to identify the underlying host mechanisms.
Sustained Upregulation of Endothelial Nox4 Mediates Retinal Vascular Pathology in Type 1 Diabetes
Authors: Tang Xixiang , Wang Jinli , Abboud Hanna E. , Chen Yanming , Wang Joshua J. , Zhang Sarah X.
Published: November 2022

NADPH oxidase 4 (Nox4) is a major source of reactive oxygen species (ROS) in retinal endothelial cells (ECs) and is upregulated under hyperglycemic and hypoxic conditions. However, the role of endothelial Nox4 upregulation in long-term retinal blood vessel damage in diabetic retinopathy (DR) remains undefined. Here, we attempted to address this question using humanized EC-specific Nox4 transgenic (hNox4EC-Tg) and EC-specific Nox4 knockout (Nox4EC-KO) mouse models. Our results show that, hNox4EC-Tg mice at age of 10-12 months exhibited increased tortuosity of retinal blood vessels, focal vascular leakage, and acellular capillary formation. In vitro study revealed enhanced apoptosis in brain microvascular ECs (BMECs) derived from hNox4EC-Tg mice, concomitant with increased mitochondrial ROS, elevated lipid peroxidation, decreased mitochondrial membrane potential (ΔΨm), and reduced mitochondrial respiratory function. In contrast, EC-specific deletion of Nox4 decreased mitochondrial ROS generation, alleviated mitochondrial damage, reduced EC apoptosis, and protected the retina from acellular capillary formation and vascular hyperpermeability in a streptozotocin-induced diabetes mouse model. These findings suggest that sustained upregulation of Nox4 in the endothelium contributes to retinal vascular pathology in diabetes, at least in part, through impairing mitochondrial function. Normalization of Nox4 expression in ECs may provide a new approach for prevention of vascular injury in DR. 
Effect of the Viscosity and Application Time of Different Phosphoric Acids on the Bond Strength and Nanoleakage of Fiber Posts to Root Dentin
Authors: Ñaupari-Villasante R , Ocampo JB , Cardenas AFM , Muniz LP , Siqueira FSF , Moreira ACL , Feitosa VP , Loguercio AD
Published: November 2022

Purpose

To evaluate the effect of application time and viscosity of meta-phosphoric acid (MPA) and ortho-phosphoric acid (OPA) on the push-out resin-dentin bond strength (PBS) and nanoleakage (NL) at the adhesive-root dentin interface.

Methods and Materials

Ninety-six roots of premolars were endodontically prepared and randomly assigned into groups according to the (i) acid (OPA and MPA), (ii) viscosity (gel and liquid), and (iii) application time (7 and 15 seconds). Fiber posts were cemented to the roots, which were then transversally sectioned into serial slices. The slices (cervical, medium, and apical) were subjected to PBS or NL. Analysis of the dentin etching pattern was performed using scanning electron microscopy (SEM). The PBS (MPa) and NL (%) data were subjected to three-way repeated-measures analysis of variance (ANOVA) and Tukey’s test (α=0.05).

Results

The cross-product interaction was significant for both PBS and NL (p<0.001). No significant difference in the PBS was observed with the use of OPA compared to MPA (p>0.05). Higher PBS values were observed in the groups etched for 15 seconds compared to 7 seconds, regardless of the acid, viscosity, or root third (p<0.05). Lower NL was observed for MPA etching when compared to OPA etching (p<0.05), and these values were not affected by increasing the application time or acid viscosity (p>0.05). A more pronounced etching pattern was observed with OPA than MPA regardless of the acid viscosity.

Conclusion

The use of 40% meta-phosphoric acid promoted adequate bond strength without increasing dentin demineralization or void spaces in the hybrid layer.
A Demographic Profile of Inflammatory Bowel Disease in Uttar Karnataka
Authors: S Hajare
Published: November 2022

Inflammatory bowel disease (IBD) results from production of dysregulated gut mucosal immune response to luminal antigens. Scientific literature pertaining to Southern part of Indian is limited, especially with focus on demographic and clinical phenotypes of IBD. Hence, the present study is aimed at evaluating these factors in a geographic area of Uttar Karnataka in two types of IBD- Ulcerative colitis (UC) and Crohn’s disease (CD). Methodology: A retrospective study was done with 91 patients with IBD from 2009 to 2019. Patients were routinely started on treatment with local and systemic 5-aminosalicylic acid derivatives. Different line of therapies was administered to both UC and Crohn’s disease with different drugs or combination of drugs. Results: Of the 91 patients, 85 were having UC and 6 with CD. The mean age of patients with UC and CD was 41.8 years and 52.2 years; male predominance was observed in UC group of patients in contrast to CD group. UC patients were found with Extraintestinal manifestation i.e. Erythema nodosum (0.01%), Type 1 peripheral arthritis (0.01%), Type 2 peripheral arthritis (0.01%). A total of 6 UC patients required surgery for various causes, with the majority being males, including 2 of them who developed cancer. Conclusion: There was a predominance of patients with UC, especially in men. The age, origin and level of education may interfere with early diagnosis. This geographical region survey provides an opportunity for understanding possible etiopathogenetic factors associated with the disease and pattern of treatment.
The Use of Social Media as a Water Tourism Destination Promotional Media at Banjar Hot Spring: Digital Marketing Perspective
Authors: Kusuma Putu Nata , Trianasari Trianasari , Rahmawati Putu Indah
Published: November 2022

This research aimed at exploring how social media was used to promote a tourist destination and how tourists perceive the related content of the social media. The study was conducted at the Tourism Department of Buleleng Regency and the tourism site of Banjar Hot Spring. To meet the fistt research objective, data were collected from three officers and a head of maketing division. The participants were recruited using purposive sampling technique with the criteria of having involvement in the management of social media. Data were collected using observation, interview, and focus group discussion. For the second research question, 38 tourists voluntarily participated. Data were gathered using open-ended-questions method. A qualitative approach was employed for data analysis. The findings suggested that the Tourism Department has demonstrated an optimal utilisation of social media to promote Banjar Hot Spring. However, the use of story telling and type writing may enhance the performance of social media. The second data analysis revealed that tourists perceived the social media was satisfactory.
The Influence of Leadership Style, Communication, and Work Discipline on Employee Performance of PT Sinar Gunung Sawit Raya
Authors: Purba Purnama Yanti , Houten Ricky Van , Siahaan Nadessah Riama Sello , Nababan Marco Yeremia , Sinaga Dolly Christian
Published: November 2022

Amid the Covid-19 pandemic, economic growth in various business sectors worldwide has decreased. The background of this research is the phenomenon of not achieving production targets, leaders who are too strict with subordinates, communication between employees is not good, and lack of discipline and self-awareness of employees in carrying out regulations. This study aimed to examine and analyze the effect of Leadership Style, Communication, and Work Discipline on Employee Performance at PT Sinar Gunung Sawit Raya. The approach in this study is a quantitative descriptive approach. The population totals 140 employees. The technique of determining the sample is simple random sampling. Collecting data in this study using observation, questionnaires, and documentation studies. The data analysis method used is multiple linear regression. Partial test results Leadership style has a positive and significant effect on employee performance. Communication has a positive and significant effect on employee performance, and work discipline has a positive and significant effect on employee performance at PT Sinar Gunung Sawit Raya. Simultaneously Leadership Style, Communication, and Work Discipline positively and significantly affect Employee Performance at PT. Rays of Gunung Sawit Raya.
Meningkatkan Motivasi Belajar dan Karakter Berbudaya Melalui LKPD Interaktif Menggunakan Model Predict Observe Explain Berbasis Etnomatematika Kelas I SD
Authors: Dewi Anak Agung Ayu Laksmi , Agustika Gusti Ngurah Sastra
Published: November 2022

Bahan ajar yang masih bersifat konvensional dan kurangnya penanaman konsep dengan mengaitkan materi matematika terhadap lingkungan sekitar mengakibatkan semangat belajar siswa menurun sehingga tujuan pembelajaran kurang tercapai secara maksimal. Maka dari itu, diperlukan sebuah bahan ajar yang dapat membantu siswa untuk memahami materi pembelajaran. Tujuan dari penelitian ini adalah untuk menciptakan bahan ajar berupa LKPD interaktif menggunakan model POE berbasis etnomatematika untuk siswa kelas 1 SD. Jenis dari penelitian ini adalah pengembangan dengan menggunakan model ADDIE. Subjek pada penelitian ini terdiri dari 1 orang ahli materi pembelajaran, 1 orang ahli desain dan media pembelajaran, 3 orang uji coba perorangan, 9 orang uji coba kelompok kecil, serta 31 orang uji coba kelompok besar. Metode pengumpulan data menggunakan wawancara dan kuesioner. Teknik analisis data menggunakan analisis deskriptif kualitatif dan kuantitatif. Hasil penelitian ini yaitu dari uji ahli materi pembelajaran mendapatkan skor 94,64%, ahli desain pembelajaran mendapatkan skor 93,75%, ahli media pembelajaran 97,05%, uji coba perorangan 96,15%, hasil uji coba kelompok kecil 94,44%, serta uji coba kelompok besar 91,65%. Jadi, berdasarkan hasil uji coba produk didapatkan bahwa produk yang diciptakan layak diterapkan dalam proses pembelajaran. Implikasi dari penelitian ini adalah ketersediaan bahan ajar dapat membantu siswa serta dapat digunakan sebagai penunjang aktivitas pembelajaran matematika.
Design of Digital Squeeze Frame Console for Public Service Advertisement
Authors: Astin Novita , Ceraka Nandhita Ayundra Wulintang
Published: November 2022

Digital advertisement is one of the promotional media used by producers to embed brands in the memory of their consumers. However, in the current era, digital advertising is not only used to promote a brand but in delivering public service advertisements, digital advertising is often used. This is because, with the development of the digital era, digital advertising is accepted by the public more quickly. There are various types of digital advertising, including Squeeze Frame. The process of running Squeeze Frame using the Vmix software. Vmix software is a virtual mixer that runs using a keyboard and mouse. In a broadcasting process, especially in outdoor areas, long distances in the process of controlling audio-video broadcasting become one of the obstacles. So there are limitations in running the Vmix software which can only be run using the keyboard and mouse. In this research, an alternative tool is created that can be used when running Vmix software in console form. The form of console hardware is easy to carry so that the broadcasting process using Vmix software can be carried out more easily. By using Arduino Uno CH340 as a microcontroller, the console not only adjusts the audio-video input but also adjusts the squeeze frame display. The result of this research is that the hardware console runs well, for the squeeze frame merging button for public service advertisements. Respondents are interested in this console with a percentage of 95.8%
Usability Test for User Centered Design-Based Industrial Internship Management System
Authors: Maharrani Ratih Hafsarah , Supriyono Abdul Rohman , Syafirullah Lutfi , Purwanto Riyadi
Published: November 2022

Internship is one of the efforts to align the knowledge or competencies obtained during the lecture with the world of work directly. In this research will be built an industrial internship management system starting from industry submissions, internship guidance and assessment of industry seminars with User Centered Design. This UCD method focuses on designing user-oriented systems. The results of the study were conducted with a usability test with 20 items (items) and 19 respondents, it can be concluded that each component of usability gets a value of > 80% which means it has a very good predicate, while the overall usability test of 87.69% includes a very good predicate.
Development of Interactive Multimedia for Room Service Subject
Authors: Sikumbang Syaidina Putri , Yulianti Yeni , Riska Nur
Published: November 2022

In order to create optimal learning, learning media with new variations that are interesting but effective and  advanced technology are needed. This study aims to develop learning media in the form of interactive multimedia based on Adobe Flash CS6 that is suitable for room service on course restaurant setting and service. This media is developed by research and development (R&D) and using the APPED (Analysis, Planning, Production, Evaluation, Dissiminate) as the development model. The results which is quantitative data are then converted into desctiptive analysis. The validation results by experts such as: 1) media expert the percentage is 99.2% which means that it is excellent quality, 2) by subject expert the percentage is 95% which means that it is excellent quality, 3) by language expert the percentage is 77.5% which means that it is good quality. For student acceptance responses, the percentage is 89.83% which means it is very positive In conclusion this media is validated to be used with good quality
Communication strategy of The Regional Agency for Disaster Management of Sumbawa Regency in disaster mitigation
Authors: Anshori Muhammad Syukron , Hatima Husnul , Lestari Ajeng
Published: November 2022

Disaster management is needed to minimize the impact of disasters. It aims to prepare and educate people living in disaster-prone areas to be better prepared to respond to the impact of disasters. Sumbawa Regency is an area prone to natural disasters such as earthquakes, floods, fires, landslides, and others. The Regional Agency for Disaster Management (BPBD) of Sumbawa Regency is to implement a program called "Desa Tangguh Bencana" to improve disaster mitigation management in Sumbawa. This study aims to determine the communication strategy of disaster mitigation planning by BPBD of Sumbawa Regency, early 2020–2021. This study uses descriptive methods and data collection techniques by observation and in-depth interviews with the initiators of the program and their effectiveness in tackling the impact of disasters. The study results showed that communication strategy is the main point of successful program implementation by selecting communicators with scientific credibility, expertise, professionals related to disasters, and good public speaking skills. The targets are the village government and, of course, the people who directly feel the impact of the disaster. A communication platform is used to introduce the program to the community using face-to-face meetings such as outreach and campaigns through social media, using a website network to reach the general public to get information regarding the program's presence. The content of the information submitted in this program is information related to disaster mitigation, stages, and implementation carried out by BPBD in dealing with disasters for people whose living areas are often affected by natural disasters.
MOTIVASI ANGGOTA IPRAHUMAS INDONESIA DALAM PENULISAN KARYA TULIS ILMIAH
Authors: Nugraha Aat Ruchiat , Hafiar Hanny
Published: November 2022

Professional development must be carried out to increase the value of quality and the professional welfare of functional workers in government institutions. Professional development efforts can be made by writing published scientific papers (KTI). The existence of scientific work becomes essential and is a prerequisite for those who want to improve the hierarchy of ranks and positions in government institutions. Scientific works published in scientific journals on behalf of individuals and groups that discuss social and public relations phenomena can be used as the performance standards barometer for government public relations functional employees to get income incentives. This study aims to identify and explain the instructional communication strategies and motivations of the Iprahumas Training participants in writing scientific papers. The research method used is the form of a survey in the form of a census of all participants of the training in writing scientific papers, namely the public relations institutions who are members of the Indonesian Public Relations Official Association (Iprahumas). The data collection technique was carried out by giving questionnaires to the participants, which were equipped with observation guidelines, interviews, and literature studies. Research shows that the motivation of Iprahumas scientific papers writing training participants is still low, namely 71.4% are unable to write scientific papers. That is because participants have limited knowledge and experience writing scientific papers in scientific journals. The participants tried to be able to implement communication strategies in the form of continuous technical guidance regularly with mentors through the WhatsApp social media group. The study's conclusion showed that the participants' understanding of scientific writing through scientific writing training was still low, which had implications for the participants' motivation to decrease. Only a few participants prepared the type of scientific article writing during the scientific writing workshop.
A close-up of bibliographic coupling on organizational communication in sports research
Authors: Abdullah Khairul Hafezad , Sofyan Davi , Rojo Jeferson Roberto , Hammood Waleed Abdulmaged
Published: November 2022

The emergence of sport sociology related to sport and communication spurred a gradual entry into academic research. This study uses bibliometric analysis of bibliographic coupling, a phenomenon that occurs when two texts quote the same source. The purpose of this study is to explore publishing trends using the unit of analysis bibliographical coupling on organizational communication in sports. The results indicated that 419 publications had been indexed in the Scopus database between 1981 and 2022. Articles were the most common document published in the database, accumulating 265 papers. Social Sciences is the most significant subject area, with 214 records. A paper written by Winand et al. in 2019 entitled “International sports federations’ social media communication: A content analysis of FIFA’s Twitter account” has become a document that obtained more citations accruing a total strength of 91. Notably, this analysis alludes that the International Journal of Sport Communication has become the most multiple credentials, with 240 citations. Two scholars, Anagnostopoulos, C. and Chadwick, S., were credited as successful authors. Griffith University, from Australia, advanced 67 citations. The United States became the leading country with total link strength of 3876. It can be concluded that research on this topic is expanding due to the empowerment of research methodologies and the development of new research contributions in the form of new avenues. Researchers and practitioners can increase future understanding in this field by thoroughly examining sports communication in numerous research areas.
Implementation management of corporate social responsibility in SIRCLO #BelajarBarengMentor program
Authors: Arvian Farrel Fauzan , Budiana Heru Ryanto
Published: November 2022

COVID-19 pandemic in Indonesia has impacts on disabilities. The private sector can help empower them through Corporate Social Responsibility (CSR). SIRCLO is one company that initiated CSR with Difalink through the #BelajarBarengMentor Program. During the implementation, several problems were faced by SIRCLO and Difalink, even though the proper implementation of CSR can benefit the company. This study aims to determine (1) How the Plan, (2) How the Implementation, (3) How the Checking, and (4) How the Evaluation in the Implementation Management of CSR in the #BelajarBarengMentor Program with analysis using the Hohnen CSR implementation model (Plan, Do, Check, and Improve). The method used is a qualitative descriptive study with data collection through interviews, observations, and literature studies. The validity of this research uses source triangulation. The study's results revealed similarities with the Hohnen model. On (1) Planning, assessment, and strategy development were initiated, but the research needed more depth and detail. On (2) Implementation, focus on program development and execution, but there are some technical problems and exposures. On (3) Checking; it had not been conducted effectively, and on (4) Evaluation; there are several evaluations and suggestions, such as the need for solid planning and a dedicated team. The suggestions given are (1) Planning; conducting in-depth research, (2) Implementation; making alternative implementation options based on research, (3) Checking; conducting the monitoring according to the concept of CSR, and (4) Evaluation; conducting solid planning and create a dedicated team for sustainable plans.
O Clima estável como Património Comum da Humanidade
Authors: Magalhães Paulo
Published: November 2022

Quando, no último relatório da Comissão de Direito Internacional (CDI-ONU), se afirma: “A atmosfera e o espaço aéreo são dois conceitos diferentes, que devem ser distinguidos(...)”, abre-se caminho para autonomizar a dimensão “funcional” do Sistema Terrestre, do elemento territorial “estático” da soberania. Esta evolução torna possível responder a: “O que é o Clima de um ponto de vista jurídico?”.
A atual incapacidade de retratar juridicamente a dinâmica funcional do planeta, esteve na origem do não reconhecimento do próprio bem Clima estável como Património Comum da Humanidade tendo-se optado por abordar o problema – as alterações climáticas são uma Preocupação Comum da Humanidade. Esta opção limitou a estratégia de ação a evitar/mitigar/neutralizar emissões, impedindo a internalização de benefícios que os ecossistemas realizam no bem Clima estável, pelo facto de desaparecem num vazio jurídico global. Assim é impossível a construção de uma economia capaz de cuidar/restaurar/regenerar ativamente o Clima. Hoje não existe um sistema que compense fazer emissões negativas.
Sendo um “problema”, o bem Clima ficou com titularidade indefinida. Ao não pertencer a ninguém, a tragédia dos comuns à escala global aconteceu. Recuperar implica reconhecer um património que pertence a todos, regras congruentes entre apropriação e provisão, não existentes no Acordo de Paris.