bluuebunny/crossref_metadata_2025 huggingface dataset:
Pregnant Women’s Perceptions of the Quality of Antenatal Care in a Public Hospital in Punjab, Pakistan during COVID-19: A Cross-Sectional Study
Authors: Tasneem Saima , Ozdal Macide Artac
Published: March 2023

Despite government efforts, many rural Pakistani women forgo regular antenatal visits, are unprepared for birth, and deliver at home or in private facilities, because they are dissatisfied with public health services. This study examined pregnant women’s perceptions of public health hospital prenatal care to suggest areas for improvement. Using simple random sampling, 200 pregnant women visiting a secondary care public health facility in Sargodha District, Pakistan, were enrolled in a cross-sectional study. The quality of prenatal care was assessed using a structured and validated questionnaire. Descriptive analysis and multivariate linear regression stepwise models were used. Of participants, 52% consider the services to be of poor quality. Education, income, number of living children, and long waiting time influenced the perceived prenatal care quality in the study population. Stakeholders rated existing services as suboptimal, especially in terms of staff availability and time spent, which reduces service use. Facility managers and policymakers should work to improve the quality of services to satisfy patients, encourage them to use antenatal care, and improve the health of both mother and child, especially in rural areas.
Female Medical Students’ Experiences of Sexism during Clinical Placements: A Qualitative Study
Authors: Ibrahim Darya , Riley Ruth
Published: March 2023

In the UK, more women are studying medicine than men, most of whom have experienced sexism, yet these experiences are under-researched. This qualitative study explores female medical students’ experiences of sexism on placement, impacts sustained, barriers and facilitators encountered upon reporting. A total of 17 semi-structured interviews were conducted, employing purposive sampling, snowball sampling and an inductive thematic analysis. A qualitative methodology was underpinned by the feminist social constructionist theory. Four themes were identified: 1—experiences of sexism, comprising physical and verbal harassment and microaggressions; 2—negative impacts of sexist encounters ranged from psychosocial to repercussions on learning and development; 3—systemic and attitudinal barriers to reporting; 4—recommendations to tackle sexism shaped by the views and experiences of female medical student participants. Female medical students experienced wide-ranging sexism which negatively impacted their wellbeing with negative repercussions for their training and development. The barriers to reporting need to be urgently addressed, and systems, policies and processes need to be over-hauled to sensitively, effectively and equitably manage and provide justice to students who experience and report sexism. Students need to be empowered to respond, report and be offered psychological safety in doing so. Attitudes and practices which are complicit in sustaining sexism need to be challenged and changed.
Harm and Harmony—Concepts of Nature and Environmental Practice in Japan
Authors: Bichler Regina M.
Published: March 2023

Japan is often surrounded by the myth of featuring a unique “love for nature”, and its traditional culture and lifestyle as having been “in harmony with nature” before it was corrupted by modernization and Westernization. In this paper, I employ three examples to delineate images of nature in different times of Japanese history and point out the discrepancy between discourse on nature and physical engagement with nature. I argue that the environmental destruction that peaked in the Meiji period (1868–1912) is not primarily derived from a new, dualistic Euro-American understanding of nature. Rather, I demonstrate that environmental harm was already inherent in premodern Japan and was reconcilable with the respective concepts of nature. Therefore, industrialization and the adoption of Western technology solely released the potential for large-scale environmental impact.
Comparison between the Effects of Normal Rain and Acid Rain on Calcareous Stones under Laboratory Simulation
Authors: Rodríguez Indira , Ortiz Almudena , Caldevilla Pablo , Giganto Sara , Búrdalo Gabriel , Fernández-Raga María
Published: March 2023

Acid rain is a problem that despite efforts to reduce atmospheric pollution, continues to impact not only countries where there is a high production of pollutants, but also transboundary areas to which the pollutants are transported. In addition to its effects on human health, there are also the effects on infrastructure and the consequences that this entails. This study on the effect of rain and acid rain on calcareous materials highlights the need for protection of buildings constructed from these materials, many of which are part of the cultural heritage. The effects caused by rain and acid rain were studied using a rain simulator whose features allow the simulation of natural conditions as realistically as possible. In the same way, the effect of a coating synthesized from nanomaterials, which has been shown to serve as protection against the degradation caused by rain, was analyzed. In addition to a long-lasting protection of the stone material, this type of coating avoids the need for the replacement of elements and the recurrent cleaning of degraded parts. It also avoids the accumulation of parts on the ground, the transport of personnel, work materials and raw materials, thereby contributing to the reduction of the carbon footprint and water savings, which in turn reduces the production of pollutants that cause acid rain.
Promoting Recovery from Disasters, Pandemics, and Trauma: A Systematic Review of Brief Psychological Interventions to Reduce Distress in Adults, Children, and Adolescents
Authors: Lotzin Annett , Franc de Pommereau Alicia , Laskowsky Isabelle
Published: March 2023

A substantial number of survivors of disasters, pandemics, and other severe stressors develop persistent distress that impairs mental health and well-being. However, only a few brief psychological interventions target distress or subclinical symptoms. This systematic review aimed to identify and describe brief psychological interventions to reduce distress or subclinical symptoms in survivors of disasters, pandemics, and other severe stressors. Based on a systematic literature search (MEDLINE, PsycINFO, PSYNDEX, PTSDpubs, and Web of Science), we reviewed published studies and study protocols on self-help, psychosocial support, or brief psychotherapeutic interventions to reduce distress and/or subclinical symptoms following natural hazards and man-made disasters, pandemics, or other traumatic events. We included 27 published studies or study protocols (n = 15 RCTs, n = 3 controlled pre–post studies, and n = 9 uncontrolled pre–post studies) describing 22 interventions. We found evidence for reducing psychological distress and/or subclinical symptoms in 9 out of 15 RCTs, 2 out of 3 controlled pre–post studies, and 9 out of 9 uncontrolled pre–post studies. One RCT provided evidence of increasing well-being. Innovative brief interventions have been developed to reduce distress and/or subclinical symptoms that have an emerging evidence base.
Evaluation of Survey Nonresponse in Measuring Cardiometabolic Health Risk Factors and Outcomes among Sexual Minority Populations: A National Data Linkage Analysis
Authors: Gupta Neeru , Cookson Samuel R.
Published: March 2023

Understanding cardiometabolic health among lesbian, gay, and bisexual (LGB) people is challenged by methodological constraints, as most studies are either based on nonprobability samples or assume that missing values in population-based samples occur at random. Linking multiple years of nationally representative surveys, hospital records, and geocoded data, we analyzed selection biases and health disparities by self-identified sexual orientation in Canada. The results from 202,560 survey respondents of working age identified 2.6% as LGB, 96.4% as heterosexual, and <1.0% with nonresponse to the sexual identity question. Those who did not disclose their sexual identity were older, less highly educated, less often working for pay, and less often residing in rural and remote communities; they also had a diagnosed cardiometabolic condition or experienced a cardiometabolic-related hospitalization more often. Among those reporting their sexual identity, LGB individuals were younger, more likely to smoke tobacco or drink alcohol regularly, more likely to have heart disease, and less likely to have a regular medical provider than heterosexual persons. This investigation highlighted the potential of leveraging linked population datasets to advance measurements of sexual minority health disparities. Our findings indicated that population health survey questions on sexual identity are not generally problematic, but cautioned that those who prefer not to state their sexual identity should neither be routinely omitted from analysis nor assumed to have been randomly distributed.
Influence of Zinc and Humic Acids on Dye Adsorption from Water by Two Composts
Authors: Paradelo Remigio , García Paula , González Alba , Al-Zawahreh Khaled , Barral Maria Teresa
Published: March 2023

Searching for alternative low-cost biosorbents for the removal of textile dyes from wastewater is currently an important subject of research. In this work, we have investigated how the presence of other contaminants in textile wastewaters can affect dye adsorption by biosorbents. We tested the adsorption of three dyes of different types: Basic Violet 10 (BV10), Acid Blue 113 (AB113) and Direct Blue 71 (DB71) by two different composts—municipal solid waste compost and pine bark compost—in the presence of Zn (5 mg L−1) or dissolved organic matter (100 mg humic acids L−1) in batch experiments. Dye adsorption capacity for both composts followed the following sequence: BV10 > AB113 > DB71. In general, dye sorption at the equilibrium was adequately described by the Freundlich model, but not always by the Langmuir model, which did not allow for the estimation of maximum retention capacities in all cases. In general, these were around 1 mg g−1 for DB71, 2 mg g−1 for AB113, and 40 mg g−1 for BV10. Municipal solid waste compost had slightly higher affinity than pine bark compost for the anionic dyes AB113 and DB71, whereas for the cationic dye BV10, pine bark compost presented a much higher adsorption capacity (41.7 mg g−1 versus 6.8 mg g−1). The presence of Zn or dissolved organic matter in the solutions at typical wastewater concentrations did not decrease the dye adsorption capacity of the composts. This result is positive both for the real application of composts to real textile wastewaters and for the validity of the results of biosorbent performance obtained with single-dye solutions.
Use of a Living Lab Approach to Implement a Smoke-Free Campus Policy
Authors: Mullin Martina , Allwright Shane , McGrath David , Hayes Catherine B.
Published: March 2023

While universities have increasingly become tobacco-/smoke-free, to our knowledge, no campus has reported 100% policy compliance. Innovative approaches to encourage compliance and ongoing data collection are needed. This paper describes actions undertaken, framed within a Living Lab (LL) approach, to implement smoke-free campus policies in an Irish university. The action research comprised student-collected data on observed smoking on campus to evaluate adherence and compliance, first to a smoke-free zones policy (June 2016–March 2018), and then to a smoke-free campus policy (March 2019–February 2020). From June 2016–February 2020, 2909 smokers were observed. Adherence, defined as the average reduction in number of observed smokers from baseline in May 2016, reduced by 79% from 5.7 to 4.9 . Compliance, defined as the proportion of smokers who complied when reminded of the policy, was 90% (2610/2909). Additional activities included development of a broader health promotion programme; identification of a pattern of ‘social smoking’; and promoting increased awareness of the environmental harms of tobacco. Ongoing policy implementation is essential for smoke-free policies and should include data collection and evaluation. Actions framed within the characteristics of a LL achieved fewer observed smokers. A LL approach is recommended to encourage policy adherence and compliance.
Reaction of US and Chinese Stock Markets to COVID-19 News
Authors: Lee Hock-Ann , Liew Venus Khim-Sen , Ghazali Mohd Fahmi , Riaz Samina
Published: March 2023

The COVID-19 outbreak slowed down global economic activities substantially, resulting in unrest in the financial markets, especially in the beginning of the pandemic outbreak. This study aims to investigate if COVID-19 caused abnormal returns in the US and the Chinese stock markets in the beginning of the pandemic outbreak. Event study methodology is adopted for this purpose. This study finds that a significant negative impact appeared immediately after the Wuhan lockdown in the Chinese markets, while the US markets were slow to pick up. The former was more severely hurt after lockdown, while the latter was more brutally affected after COVID-19 was labeled a global threat. COVID-19 also played a significant role in connecting these two stock markets. The US and China should collaborate further in combating this novel and notorious global pandemic.
Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts
Authors: Atiakshin Dmitri , Patsap Olga , Kostin Andrey , Mikhalyova Lyudmila , Buchwalow Igor , Tiemann Markus
Published: March 2023

The mechanisms of ovarian endometrioid cyst formation, or cystic ovarian endometriosis, still remain to be elucidated. To address this issue, we analyzed the involvement of mast cell (MC) tryptase and carboxypeptidase A3 (CPA3) in the development of endometriomas. It was found that the formation of endometrioid cysts was accompanied by an increased MC population in the ovarian medulla, as well as by an MC appearance in the cortical substance. The formation of MC subpopulations was associated with endometrioma wall structures. An active, targeted secretion of tryptase and CPA3 to the epithelium of endometrioid cysts, immunocompetent cells, and the cells of the cytogenic ovarian stroma was detected. The identification of specific proteases in the cell nuclei of the ovarian local tissue microenvironment suggests new mechanisms for the regulatory effects of MCs. The cytoplasmic outgrowths of MCs propagate in the structures of the stroma over a considerable distance; they offer new potentials for MC effects on the structures of the ovarian-specific tissue microenvironment under pathological conditions. Our findings indicate the potential roles of MC tryptase and CPA3 in the development of ovarian endometriomas and infer new perspectives on their uses as pharmacological targets in personalized medicine.
PINK1 Immunoexpression Predicts Survival in Patients Undergoing Hepatic Resection for Colorectal Liver Metastases
Authors: Celis-Pinto Juan Carlos , Fernández-Velasco Adela Alonso , Corte-Torres María Daniela , Santos-Juanes Jorge , Blanco-Agudín Noelia , Piña Batista Kelvin Manuel , Merayo-Lloves Jesús , Quirós Luis M. , Fernández-Vega Iván
Published: March 2023

PTEN-induced kinase-1 (PINK1) is the initiator of the canonical mitophagy pathway. Our aim was to study the immunoexpression of PINK1 in surgical specimens from ninety patients with metastatic colorectal adenocarcinoma (CRC) to the liver (CRLM). Tissue arrays were produced, and immunohistochemical studies were analyzed by the H-Score method. The mean immunoexpression of PINK1 in normal tissues was between 40 to 100 points. In tumoral tissues, positive PINK1 immunoexpression was observed in all samples, and no differences were noted between CRCs. In CRLMs, a significant under-expression was noted for PINK1 from the rectum (71.3 ± 30.8; p < 0.042) compared to other sites. Altered PINK1 immunoexpression in CRCs, either higher than 100 points or lower than 40 points, was associated with worse overall survival (OS) (p < 0.012) due to a shorter post-metastatic survival (PMS) (p < 0.023), and it was found to be a significant independent predictor of prognosis in a multivariate model for OS and PMS (HR = 1.972, 95% CI 0.971–4.005; p = 0.022. HR = 2.023, 95% CI 1.003–4.091; p = 0.037, respectively). In conclusion, altered PINK1 immunoexpression determined in CRCs with resected CRLM predicts a worse prognosis, possibly due to the abnormal function of mitophagy.
Editorial for Special Issue—“Early-Stage Drug Discovery: Advances and Challenges”
Authors: Sippl Wolfgang
Published: March 2023

The development of a new drug from the first hit to the launch of an approved product is a complex process that usually take around 12–15 years and costs more than USD 1–2 billion [...]
ZNF750: A Novel Prognostic Biomarker in Metastatic Prostate Cancer
Authors: Montanaro Manuela , Agostini Massimiliano , Anemona Lucia , Bonanno Elena , Servadei Francesca , Finazzi Agrò Enrico , Asimakopoulos Anastasios D. , Ganini Carlo , Cipriani Chiara , Signoretti Marta , Bove Pierluigi , Rugolo Francesco , Imperiali Benedetta , Melino Gerry , Mauriello Alessandro , Scimeca Manuel
Published: March 2023

Prostate cancer is the most frequently diagnosed cancer and the fifth leading cause of cancer death among men in 2020. The clinical decision making for prostate cancer patients is based on the stratification of the patients according to both clinical and pathological parameters such as Gleason score and prostate-specific antigen levels. However, these tools still do not adequately predict patient outcome. The aim of this study was to investigate whether ZNF750 could have a role in better stratifying patients, identifying those with a higher risk of metastasis and with the poorest prognosis. The data reported here revealed that ZNF750 protein levels are reduced in human prostate cancer samples, and this reduction is even higher in metastatic samples. Interestingly, nuclear positivity is significantly reduced in patients with metastatic prostate cancer, regardless of both Gleason score and grade group. More importantly, the bioinformatics analysis indicates that ZNF750 expression is positively correlated with better prognosis. Overall, our findings suggest that nuclear expression of ZNF750 may be a reliable prognostic biomarker for metastatic prostate cancer, which lays the foundation for the development of new biological therapies.
Decreased SIRT1 Activity Is Involved in the Acute Injury Response of Chondrocytes to Ex Vivo Injurious Mechanical Overload
Authors: Karnik Sonali , Noori-Dokht Hessam , Williams Taylor , Joukar Amin , Trippel Stephen B. , Sankar Uma , Wagner Diane R.
Published: March 2023

A better understanding of molecular events following cartilage injury is required to develop treatments that prevent or delay the onset of trauma-induced osteoarthritis. In this study, alterations to SIRT1 activity in bovine articular cartilage explants were evaluated in the 24 h following a mechanical overload, and the effect of pharmacological SIRT1 activator SRT1720 on acute chondrocyte injury was assessed. SIRT1 enzymatic activity decreased as early as 5 min following the mechanical overload, and remained suppressed for at least 24 h. The chondrocyte injury response, including apoptosis, oxidative stress, secretion of inflammatory mediators, and alterations in cartilage matrix expression, was prevented with pharmacological activation of SIRT1 in a dose-dependent manner. Overall, the results implicate SIRT1 deactivation as a key molecular event in chondrocyte injury following a mechanical impact overload. As decreased SIRT1 signaling is associated with advanced age, these findings suggest that downregulated SIRT1 activity may be common to both age-related and injury-induced osteoarthritis.
The Masticatory Activity Interference in Quantitative Estimation of CA1, CA3 and Dentate Gyrus Hippocampal Astrocytes of Aged Murine Models and under Environmental Stimulation
Authors: Cunha Feio Leal Marília da , Amaral Junior Fabio Leite do , Silva Arruda Bernardo Freire da , Kurosawa Juliana Ayumi Azevedo , Vieira Amanda Almeida , Maia Júlia Corrêa Campos , Scalfoni Viviana Virgínia Bezerra , Silveira Junior Antonio Morais da , Feijó Matheus Oliveira , Albuquerque Fernanda Beatriz Araújo de , Marta Maria Helena Moutinho , Normando Marina Paula Nobre , Silva Alana Gabriele Oliveira Cabeça da , Trindade Fernanda Catharina Pires da , Siqueira Mendes Fabíola de Carvalho Chaves de , Sosthenes Marcia Consentino Kronka
Published: March 2023

Studies indicating the influence of masticatory dysfunction, due to a soft diet or lack of molars, on impairing spatial memory and learning have led to research about neuronal connections between areas and cell populations possibly affected. In this sense, with scarce detailed data on the subfields of hippocampus in dementia neurodegeneration, there is no information about astrocytic responses in its different layers. Thus, considering this context, the present study evaluated the effects of deprivation and rehabilitation of masticatory activity, aging, and environmental enrichment on the stereological quantification of hippocampal astrocytes from layers CA1, CA3, and DG. For this purpose, we examined mature (6-month-old; 6M), and aged (18-month-old; 18M) mice, subjected to distinct masticatory regimens and environments. Three different regimens of masticatory activity were applied: continuous normal mastication with hard pellets (HD); normal mastication followed by deprived mastication with equal periods of pellets followed by soft powder (HD/SD); or rehabilitated masticatory activity with equal periods of HD, followed by powder, followed by pellets (HD/SD/HD). Under each specific regimen, half of the animals were raised in standard cages (impoverished environment (IE)) and the other half in enriched cages (enriched environment (EE)), mimicking sedentary or active lifestyles. Microscopic stereological, systematic, and random sampling approaches with an optical dissector of GFAP-immunolabeled astrocytes were done, allowing for an astrocyte numerical estimate. Stratum moleculare and hilus, from the dentate gyrus (DG) and Strata Lacunosum-Moleculare, Oriens, and Radiatum, similarly to the dentate gyrus, showed no significant change in any of the investigated variables (age, diet, or environment) in these layers. However, in Stratum radiatum, it was possible to observe significant differences associated with diet regimens and age. Therefore, diet-related differences were found when the HD 18M IE group was compared to the HD/SD/HD 18-month-old group in the same environment (IE) (p = 0.007). In the present study, we present modulatory factors (masticatory function, environmental enrichment, and aging) for the differentiated quantitative laminar response in the hippocampal regions, suggesting other studies to read the plasticity and responsiveness of astrocytes, including the molecular background.
Global Transcriptomic Analyses Provide New Insight into the Molecular Mechanisms of Endocarp Formation and Development in Iron Walnut (Juglans sigillata Dode)
Authors: Yu Anmin , Zou Hanyu , Li Ping , Yao Xiaowei , Guo Jiayu , Sun Rui , Wang Gaosheng , Xi Xueliang , Liu Aizhong
Published: March 2023

Iron walnut (Juglans sigillata Dode) is a native species in southwestern China that exhibits variation in both fruit morphology and shell thickness. However, the underlying molecular processes controlling hardened endocarp development in walnut has not yet been reported. Here, we generated transcriptional profiles of iron walnut endocarp at three developmental stages using “Dapao”, the most common commercial variety. Using pairwise comparisons between these three stages, a total of 8555 non-redundant differentially expressed genes (DEGs) were identified, and more than one-half of the total DEGs exhibited significant differential expression in stage I as compared with stage II or stage III, suggesting that the first stage may ultimately determine the final characteristics of the mature walnut shell. Furthermore, in the clustering analysis of the above DEGs, 3682, 2349, and 2388 genes exhibited the highest expression in stages I, II, and III, respectively. GO enrichment analysis demonstrated that the major transcriptional variation among the three developmental stages was caused by differences in cell growth, plant hormones, metabolic process, and phenylpropanoid metabolism. Namely, using the tissue-specific expression analysis and a gene co-expression network, we identified MADS-box transcription factor JsiFBP2 and bHLH transcription factor JsibHLH94 as candidate regulators of endocarp formation in the early stage, and JsiNAC56 and JsiMYB78 might play key roles in regulating the lignification process of endocarp in the late stage. This study provides useful information for further research to dissect the molecular mechanisms governing the shell formation and development of iron walnut.
Cycloastragenol: A Novel Senolytic Agent That Induces Senescent Cell Apoptosis and Restores Physical Function in TBI-Aged Mice
Authors: Zhang Yanghuan , Gao Dongxiao , Yuan Yang , Zheng Runzi , Sun Manting , Jia Shuting , Liu Jing
Published: March 2023

Accumulating evidence indicates that the increased burden of senescent cells (SCs) in aged organisms plays an important role in many age-associated diseases. The pharmacological elimination of SCs with “senolytics” has been emerging as a new therapy for age-related diseases and extending the healthy lifespan. In the present study, we identified that cycloastragenol (CAG), a secondary metabolite isolated from Astragalus membrananceus, delays age-related symptoms in mice through its senolytic activity against SCs. By screening a series of compounds, we found that CAG selectively kills SCs by inducing SCs apoptosis and that this process is associated with the inhibition of Bcl-2 antiapoptotic family proteins and the PI3K/AKT/mTOR pathway. In addition, CAG treatment also suppressed the development of the senescence-associated secretory phenotype (SASP) in SCs, thereby inhibiting cell migration mediated by the SASP. Furthermore, the administration of CAG for 2 weeks to mice with irradiation-induced aging alleviated the burden of SCs and improved the animals’ age-related physical dysfunction. Overall, our studies demonstrate that CAG is a novel senolytic agent with in vivo activity that has the potential to be used in the treatment of age-related diseases.
Novel Transcriptomic Interactomes of Noncoding RNAs in the Heart under Altered Thyroid Hormonal States
Authors: Rajagopalan Viswanathan , Chakraborty Sankalpa , Lin Richard
Published: March 2023

Noncoding RNAs are emerging as vital players in cardiovascular diseases. Thyroid hormones (THs) are crucial for cardiovascular survival; however, correction of systemic hypothyroidism (low serum THs) may not improve cardiac tissue-level hypothyroidism or cardiac function. Mechanistically, the understanding of noncoding transcriptomic interactions influencing TH-mediated cardiac effects is unclear. Adult C57BL/6J mixed-sex mice were randomized into Control, Hypothyroid (HypoTH), Hyperthyroid (HyperTH), and HypoTH-Triiodothyronine restoration groups. Physiological, morphological, biochemical, molecular, and whole transcriptomic studies and appropriate statistical analyses were performed. HypoTH showed significant atrophy, depressed cardiac function, and decreased serum THs versus controls, and Triiodothyronine supplementation restored them. HyperTH significantly increased serum THs with hypertrophy. Real-time PCR showed significantly altered inflammatory and immune lncRNAs. The transcriptomic sequencing revealed significant differential expressions of lncRNAs, miRNAs, and mRNAs. Eleven novel circRNAs significantly decreased with increased THs. Multiple pathways were GO-/KEGG-enriched, including cardiac, thyroid, cancer, mitochondrial, inflammatory, adrenergic, metabolic, immune-mediated, vesicular, etc. We also uncovered significant novel co-expression and interactions of lncRNA-miRNA, lncRNA-miRNA-mRNA, lncRNA-mRNA, circRNA-miRNA, and miRNA-mRNA, and splicing events. This includes a novel pathway by which the predominant cardiac TH receptor alpha may interact with specific lncRNAs and miRNAs. This is the first study reporting a comprehensive transcriptome-wide interactome in the cardiac–thyroid axis.
Ethyl Formate Fumigation for Control of the Scale Insect Asiacornococcus kaki, a Quarantine Pest on Sweet Persimmon, Diospyros kaki: Efficacy, Phytotoxicity and Safety
Authors: Kwon Tae-Hyung , Cho Jin-Hun , Kim Dong-Bin , Kwon Gi-Myon , Hong Ki-Jeong , Ren Yonglin , Lee Byung-Ho , Park Min-Goo
Published: March 2023

Sweet persimmons are a valuable export commodity. However, the presence of live insects such as Asiacornococcus kaki limits their access to many export markets. Methyl bromide, traditionally used for pest control, is damaging to human health and the environment. Ethyl formate (EF) is a viable alternative; however, its effectiveness against A. kaki on sweet persimmon fruit is unknown. We evaluated the effectiveness of EF fumigation in controlling A. kaki present under the calyx of persimmon fruit. The hatching rate of eggs and the survival rates of nymphs and adults of A. kaki at low temperatures, its LCt50 and LCt99 after EF exposure, and phytotoxic damage caused by EF were evaluated in laboratory-scale and commercial-scale tests. The dose–response tests showed that the EF LCt99 at 5 °C was 9.69, 42.13, and 126.13 g h m−3 for adults, nymphs, and eggs, respectively. Commercial-scale tests demonstrated EF efficacy against all A. kaki stages without causing phytotoxic effects on persimmons, though the eggs of A. kaki were not completely controlled in linear low-density polyethylene (LLDPE)-packaged fruit. This study demonstrated that EF is a potential fumigant for quarantine pretreatment, especially before persimmon fruit is packed with LLDPE film, to control A. kaki infesting sweet persimmon fruit.
First Record of the Invasive Scale Insect, Pulvinaria hydrangeae Steinweden, 1946 (Hemiptera: Coccomorpha: Coccidae) in Romania
Authors: Paraschiv Marius
Published: March 2023

Over the last few decades, globalization and global trade have increased the risk of the vehiculation of invasive organisms, which has had multiple negative effects, both economic and ecological. Through this study, we aimed to produce a report on the first record of the invasive scale insect Pulvinaria hydrangeae (Stein. 1946) in Brașov County in central Romania. It was found on two native tree species: sycamore (Acer pseudoplatanus) and linden (Tilia cordata). In this paper, we (i) highlight the list of possible hosts, (ii) provide a general outlook on infestations and (iii) review the control options for this particular pest. Because early detection and quick reporting are the most important actions in the successful management of invasive species, in general, we also provide a synthetic morphological description of the adult female specimens and ovisacs. Due to natural occurrence, our findings highlight the potential risks posed by the infestation of this insect to native tree species belonging to the Acer and Tilia genera. Because of the temperate climate in Romania and the fact that females are wingless, the new infestations will probably be made through the vehiculation of infested plants, rather than through natural spreading. However, because of global warming, the chances of this species surviving during the winter are likely to increase, making northern expansion of the cottony hydrangea scale feasible.
Winners and Losers: Cordulegaster Species under the Pressure of Climate Change
Authors: Fekete Judit , De Knijf Geert , Dinis Marco , Padisák Judit , Boda Pál , Mizsei Edvárd , Várbíró Gábor
Published: March 2023

(1) Bioclimatic factors have a proven effect on species distributions in terrestrial, marine, or freshwater ecosystems. Because of anthropogenic effects, the changes in these variables are accelerated; thus, the knowledge of the impact has great importance from a conservation point of view. Two endemic dragonflies, the Balkan Goldenring (Cordulegaster heros) and the Two-Toothed Goldenring (C. bidentata), confined to the hilly and mountainous regions in Europe, are classified as “Near Threatened” according to the IUCN Red List. (2) Modeling the potential occurrence of both species under present and future climatic conditions provides a more accurate picture of the most suitable areas. The models were used to predict the responses of both species to 6 different climate scenarios for the year 2070. (3) We revealed which climatic and abiotic variables affect them the most and which areas are the most suitable for the species. We calculated how future climatic changes would affect the range of suitable areas for the two species. (4) According to our results, the suitable area for Cordulegaster bidentata and C. heros are strongly influenced by bioclimatic variables and showed an upward shift toward high elevations. The models predict a loss of suitable area in the case of C. bidentata and a large gain in the case of C. heros.
Application of Semi-Circular Double-Skin Facades in Auditoriums in Winter Conditions
Authors: Conceição Maria Inês , Conceição Eusébio , Lúcio Maria Manuela , Gomes João , Awbi Hazim
Published: March 2023

The DSF (double-skin facade) system is an important element in building design and is used in adjacent spaces to control the inlet solar radiation, heat the air, reduce energy consumption, decrease the acoustics levels, and produce photovoltaic energy, among other improvements. The DSF system can, for example, be used in winter conditions to heat the air, which is then transported to non-adjacent spaces to improve the thermal comfort level and the indoor air quality that the occupants are subjected to. Smooth DSF systems, which are a focus in the literature, are subjected to higher solar radiation levels at a specific hour of the day. The semi-circular DSF system used in this work, which was built from a group of smooth DSF systems with different orientations, guarantees the reception of the highest incident solar radiation throughout the entire day. This work presents a numerical study of a new DSF system, called the semi-circular DSF. The DSF system consists of a set of 25 smooth DSFs with different orientations, each one consisting of an outer glazed surface and an inner surface provided by the outer facade of the auditorium, both separated by an air channel. In this work, the influence of the radius of the semi-circular DSF system and the opening angle of the DSF system on the thermal response of the auditorium was analysed. Thus, six auditoriums were considered: two sets of three auditoriums with radii of 5 m and 15 m, with each of the auditoriums having a different DSF opening angle (45°, 90°, and 180°). It was found that the greater the radius of the semi-circular DSF and the opening angle of the DSF system, the greater the area of its glazed surface and, consequently, the greater the availability of solar heating power. Therefore, during the occupation period, only the set of auditoriums with the largest semi-circular DSF radius managed to present acceptable levels of thermal comfort, which were verified from mid-morning until late afternoon. As for the opening angle of the DSF system, the influence was not very significant, although slight improvements in thermal comfort were noted when the value of this angle was reduced (see Case F as an example) due to the corresponding decrease in the volume of indoor air to be heated. In all auditoriums (see Case A to Case F), it was verified that the indoor air quality was acceptable for the occupants, so the airflow rate was adequately promoted by the ventilation system.
Incidence of Healthcare-Associated Infections in a Neonatal Intensive Care Unit before and during the COVID-19 Pandemic: A Four-Year Retrospective Cohort Study
Authors: Ceparano Mariateresa , Sciurti Antonio , Isonne Claudia , Baccolini Valentina , Migliara Giuseppe , Marzuillo Carolina , Natale Fabio , Terrin Gianluca , Villari Paolo ,   The Collaborating Group
Published: March 2023

The COVID-19 pandemic may have had an impact on healthcare-associated infection (HAI) rates. In this study, we analyzed the occurrence of HAIs in a neonatal intensive care unit (NICU) of the Umberto I teaching hospital in Rome before and during the pandemic. All infants admitted from 1 March 2018 to 28 February 2022 were included and were divided into four groups according to their admission date: two groups before the pandemic (periods I and II) and two during the pandemic (periods III and IV). The association between risk factors and time-to-first event was analyzed using a multivariable Cox regression model. Over the four-year period, a total of 503 infants were included, and 36 infections were recorded. After adjusting for mechanical ventilation, birth weight, sex, type of delivery, respiratory distress syndrome, and previous use of netilmicin and fluconazole, the multivariable analysis confirmed that being hospitalized during the pandemic periods (III and IV) was the main risk factor for HAI acquisition. Furthermore, a change in the etiology of these infections was observed across the study periods. Together, these findings suggest that patient management during the pandemic was suboptimal and that HAI surveillance protocols should be implemented in the NICU setting promptly.
Disparities in the Prevalence and Risk Factors for Carotid and Lower Extremities Atherosclerosis in a General Population—Bialystok PLUS Study
Authors: Lisowska Anna , Dubatówka Marlena , Chlabicz Małgorzata , Jamiołkowski Jacek , Kondraciuk Marcin , Szyszkowska Anna , Knapp Małgorzata , Szpakowicz Anna , Łukasiewicz Adam , Kamiński Karol
Published: March 2023

This study was conducted in a representative sample of area residents aged 20–80 years old. The aim of the study was to assess the prevalence of classic risk factors of atherosclerosis in the studied population and to search for new risk factors in these patient subpopulations. A total of 795 people (mean age 48.64 ± 15.24 years, 45.5% male) were included in the study group. Two independent data analyses were performed. In the first analysis, the study group was divided into two subgroups depending on the presence or absence of atherosclerotic plaques in carotid arteries (APCA). APCA were observed in 49.7% of the study group: in the population aged between 41 and 60 years in 49.3%, and those between 61 and 70 years in 86.3%. Patients with APCA were more often diagnosed with arterial hypertension, diabetes, and hypercholesterolemia. In the second analysis, the study group was divided into two subgroups depending on the presence of lower extremities atherosclerotic disease (LEAD). Patients with an ABI (ankle-brachial index) ≤ 0.9 constituted 8.5% of the study group, and they were significantly older, and more often diagnosed with diabetes and APCA. To identify the factors most strongly associated with APCA and an ABI ≤ 0.9, logistic regression was used, with stepwise elimination of variables. The strongest factors associated with APCA were current smoking and diastolic central pressure. We did not note such an association and did not find additional parameters to facilitate the diagnosis of LEAD in asymptomatic patients. The most important observation in our study was the high prevalence of APCA in the study population, especially in the group of young people under the age of 60.
The Annoying Nature of Snoring Sounds Is Not Only about Intensity: A Pilot Study on Exposed Test Subjects
Authors: Huang Zhengfei , Aarab Ghizlane , Ravesloot Madeline J. L. , de Vries Nico , Hilgevoord Antonius A. J. , Lobbezoo Frank
Published: March 2023

This study aims to assess (i) which acoustic characteristics of snoring sounds are associated with the annoying nature of snoring sounds; (ii) whether listeners’ heart rates correlate with their perceived annoyance; and (iii) whether perceived annoyance is different between listeners with different experiences with their bedpartners’ snoring sounds. Six snoring epochs with distinct acoustic characteristics (viz., reference, high pitch, high intensity, short interval, irregular intensity, and irregular intervals) were collected from snoring patients. Twenty physicians and technicians were involved in the healthcare of snoring patients, and were divided into three groups based on personal experience with their bedpartners’ snoring sounds (viz., non-snoring, snoring but not annoying, and snoring and annoying). The test subjects listened to each epoch and rated its level of annoyance. Listeners’ heart rates were also recorded during the test using a finger plethysmograph. Within the limitations of this study, it was found that, compared with other snoring sounds, snoring sounds with high intensity and irregularity were associated with higher perceived annoyance. However, higher perceived annoyance of snoring sound was not reflected in heart rate-related parameters. In addition, listeners’ personal experiences do not seem to affect their perceived annoyance.
Effect of Mg Contents on the Microstructure, Mechanical Properties and Cytocompatibility of Degradable Zn-0.5Mn-xMg Alloy
Authors: Yang Lingbo , Li Xing , Yang Lijing , Zhu Xinglong , Wang Manli , Song Zhenlun , Liu Huinan Hannah , Sun Wensheng , Dong Ruihong , Yue Jiqiang
Published: March 2023

The effect of magnesium (Mg) content on the microstructure, mechanical properties, and cytocompatibility of degradable Zn-0.5Mn-xMg (x = 0.05 wt%, 0.2 wt%, 0.5 wt%) alloys was investigated. The microstructure, corrosion products, mechanical properties, and corrosion properties of the three alloys were then thoroughly characterized by scanning electron microscopy (SEM), electron back-scattered diffraction (EBSD), and other methods. According to the findings, the grain size of matrix was refined by the addition of Mg, while the size and quantity of Mg2Zn11 phase was increased. The Mg content could significantly improve the ultimate tensile strength (UTS) of the alloy. Compared with the Zn-0.5Mn alloy, the UTS of Zn-0.5Mn-xMg alloy was increased significantly. Zn-0.5Mn-0.5Mg exhibited the highest UTS (369.6 MPa). The strength of the alloy was influenced by the average grain size, the solid solubility of Mg, and the quantity of Mg2Zn11 phase. The increase in the quantity and size of Mg2Zn11 phase was the main reason for the transition from ductile fracture to cleavage fracture. Moreover, Zn-0.5Mn-0.2Mg alloy showed the best cytocompatibility to L-929 cells.
Early Postoperative Recovery after Modified Ultra-Minimally Invasive Sonography-Guided Thread Carpal Tunnel Release
Authors: Mende Konrad , Kamphuis Saskia J. M. , Schmid Valentin , Schaefer Dirk J. , Kaempfen Alexandre , Gohritz Andreas
Published: March 2023

Thread carpal tunnel release (TCTR) has been reported to be safe and effective for the treatment of carpal tunnel syndrome. The aim of this study is to evaluate the modified TCTR for safety, efficacy, and postoperative recovery. Seventy-six extremities in 67 patients undergoing TCTR were analyzed pre- and postoperatively using clinical parameters and patient-reported outcome measures. Twenty-nine men and 38 women with a mean age of 59.9 ± 18.9 years underwent TCTR. The mean postoperative time to resume activities of daily living was 5.5 ± 5.5 days, analgesia was completed after 3.7 ± 4.6 days, and return to work was achieved after a mean of 32.6 ± 15.6 days for blue-collar workers and 4.6 ± 4.3 days for white-collar workers. The Boston Carpal Tunnel Questionnaire (BCTQ) and Disability of Arm, Shoulder, and Hand (DASH) scores were comparable with previous studies. Overall, two persistent compressions and one recurrence required open reoperation (3.9%). All three had been operated in the initial phase, and none required reoperation after an additional safety step was introduced. No other complications occurred. TCTR surgery appears to be a safe and reliable technique with almost no wound and scarring and a potentially faster recovery time than open techniques. Although our technical modifications may reduce the risk of incomplete release, TCTR requires both ultrasound and surgical skills and has a considerable learning curve.
Exploring the Clinical Efficacy of Venous Thromboembolism Management in Saudi Arabian Hospitals: An Insight into Patient Outcomes
Authors: Bakhsh Ebtisam , Shaban Mostafa , Al Subaie Sarah , Al Moshary May , AlSheef Mohammed
Published: March 2023

Venous thromboembolism (VTE) is a common condition that can recur, leading to multiple therapeutic strategies to prevent it. The aim of this study was to explore the clinical efficacy of VTE management in Saudi Arabian hospitals and to gain insights into patient outcomes. A retrospective single-center study was conducted that retrieved the data of all patients with VTE registered from January 2015 to December 2017. Patients of all age groups were included if they attended the thrombosis clinic at KFMC during the data collection period. The study analyzed the various therapeutic strategies used for VTE and their effect on patient outcomes. The results showed that 14.6% of the patients had provoked VTE, with a higher incidence among females and younger patients. The most commonly prescribed treatment was combination therapy, followed by warfarin, oral anticoagulants, and factor Xa inhibitor. Despite being prescribed treatment, 74.9% of the patients experienced recurrence of VTE. There was no associated risk factor for recurrence in 79.9% of the patients. Thrombolytic therapy and catheter-directed thrombolysis were found to be associated with a lower risk of VTE recurrence, while anticoagulation therapy, including oral anticoagulants, was associated with a higher risk. Vitamin K antagonist (warfarin) and factor Xa inhibitor (rivaroxaban) had a significant positive association with VTE recurrence, while the use of a direct thrombin inhibitor (dabigatran) showed a lower risk, but it was not statistically significant. The results of the study highlight the need for further research to determine the most effective therapeutic strategy for VTE management in Saudi Arabian hospitals. The findings also suggest that anticoagulation therapy, including oral anticoagulants, may increase the risk of VTE recurrence, while thrombolytic therapy and catheter-directed thrombolysis may lower the risk.
Does the Size of the Business Still Matter, or Is Profitability under New Management, by Order of the COVID-19?
Authors: Blazek Roman , Durana Pavol , Michulek Jakub , Blazekova Kristina
Published: March 2023

Businesses should come up with a strategy, plans, and goals so that their total assets can make a profit during the transformation process. Utilizing various features of a property can generate this income. This comparison provides evidence of profitability. During the global economic downturn, a number of businesses encountered issues that caused their payment situations and profitability to deteriorate. The goal of this article is to ascertain whether particular profitability indicators also revealed the pandemic-related global crisis, particularly in the Visegrad Group countries. This analysis was conducted based on categories of business size. Specifically, 8671 enterprises were analyzed. The evaluation of indicators revealed whether there was a significant change in a negative direction, a significant change in a positive direction, or no significant change. It was possible to make a clear diagram of the companies that took part in the study and to figure out the median values in order to compare the results of the chosen profitability indicators. Correspondence analysis was conducted so that conclusions could be more accurate. According to the findings of this study, indicators of ROA, ROE, and ROS did not change significantly across enterprise size categories in the years preceding, during, and after the pandemic. Since the government regulations of the V4 countries had a significant impact on these businesses, the change was most obvious in the case of small businesses within the ROS indicator. The added value of the article is derived from its analysis of selected profitability indicators in the largest group of Central European nations and its relevance.