bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomechanical estimation of tennis serve using inertial sensors: A case study
Authors: Brocherie Franck , Dinu Daniel
Published: December 2022

Inertial measurement units may provide a relevant on-court 3-Dimension measurement system for tennis serve biomechanical analysis. Therefore, this case study aimed to report the feasibility of inertial measurement unit's kinematic and kinetic data collection during tennis serve. Two injury-free highly-trained tennis players were equipped with the inertial measurement unit (Xsens MVN suit) and performed 2 trials of five flat “first” serves on a 1 m2 target zone bordering the service box of an indoor GreenSet® tennis court surface. With the exception of the center of gravity rotation at the loading stage, all joint (shoulder, elbow, knee) angles, center of mass displacements and rotations followed a similar development for both female and male participants from loading to finish stages. At ball contact stage, articular moments (mid-trunk, upper-trunk, shoulder, elbow, wrist) and segmental contribution (pelvis linear, pelvis rotation, trunk, shoulder, elbow, wrist) repartitions also showed a comparable movement. From loading to finish stages, total, lower and upper energy contribution were similar for both players, with coefficient of variations deemed acceptable between the two trials. This inertial measurement unit appears suitable for on-court tennis serve biomechanical data collection and subsequent analysis to provide tennis players and practitioners tailored feedbacks to facilitate motor learning process and develop serve efficiency.
High Precision Magnetic Levitation Actuator for Micro-EDM
Authors: Luan Boran , Zhang Xiaoyou , Xu Fangchao , Yang Guang , Jin Junjie , Xu Chengcheng , Sun Feng , Oka Koichi
Published: December 2022

Aiming at the efficiency and precision in micro electrical discharge machining (micro-EDM) is affected because the interpole voltage is unstable in conventional micro-EDM. This paper describes a five-degrees-of-freedom (5-DOF) controlled, wide-bandwidth, and high-precision magnetic levitation actuator. The conventional micro-EDM can install the actuator to maintain a stable interpole voltage between the electrode and workpiece to realize the high-speed micro-EDM. In this paper, the structure of the magnetic levitation actuator is designed, and the magnetic field characteristics are analyzed. On this basis, an integrator and regulator are used along with a controller with local current feedback to eliminate steady-state errors, stabilize the control system, and improve the bandwidth and positioning accuracy of the magnetic levitation actuator, and the dynamic performance of the actuator is evaluated. The experimental results show that the developed actuator has excellent positioning performance with micron-level positioning accuracy to meet the demand for the real-time, rapid, and accurate adjustment of the interpole gap during micro-EDM.
Polysaccharide of Atractylodes macrocephala Koidz Alleviates Cyclophosphamide-Induced Thymus Ferroptosis in Gosling
Authors: Zhou Xiangying , Cao Nan , Xu Danning , Tian Yunbo , Shen Xu , Jiang Danli , Huang Yunmao , Li Wanyan , Li Bingxin
Published: December 2022

The present study aimed to explore the mechanism by which PAMK alleviates cyclophosphamide (CTX)-induced ferroptosis in thymocytes. One-day-old goslings were divided into four groups (10 goslings/group). The CON and CTX groups were fed a basic diet. The PAMK and CTX + PAMK groups were fed the basic diet mixed with PAMK (400 mg/kg). Moreover, the CTX and CTX + PAMK groups were given a daily injection of 40 mg/kg BW of CTX (at 19, 20, and 21 days of age). On the other hand, the CON and PAMK groups were given 0.5 mL of sterilized saline into the leg muscle (at 19, 20, and 21 days of age). The goslings were fed for 28 days. The ferroptosis pathway was enriched in transcriptome sequencing. Compared to the CON group, the thymus in the CTX group underwent injury, and the mitochondria of thymocytes showed features of ferroptosis. PAMK treatment alleviated ferroptosis in thymocytes and thymus injury, and CTX-induced elevated levels of oxidative stress and iron content restored GPX4 protein expression (p < 0.05) and inhibited the CTX-induced activation of the ferroptosis pathway (p < 0.05). Conclusively, PAMK could reduce thymus injury by alleviating CTX-induced thymocyte ferroptosis in gosling to alleviate the immunosuppression caused by CTX in the organism.
Developing Strategies to Help Bee Colony Resilience in Changing Environments
Authors: Dequenne Isabelle , Philippart de Foy Jean-Michel , Cani Patrice D.
Published: December 2022

Climate change, loss of plant biodiversity, burdens caused by new pathogens, predators, and toxins due to human disturbance and activity are significant causes of the loss of bee colonies and wild bees. The aim of this review is to highlight some possible strategies that could help develop bee resilience in facing their changing environments. Scientists underline the importance of the links between nutrition, microbiota, and immune and neuroendocrine stress resistance of bees. Nutrition with special care for plant-derived molecules may play a major role in bee colony health. Studies have highlighted the importance of pollen, essential oils, plant resins, and leaves or fungi as sources of fundamental nutrients for the development and longevity of a honeybee colony. The microbiota is also considered as a key factor in bee physiology and a cornerstone between nutrition, metabolism, growth, health, and pathogen resistance. Another stressor is the varroa mite parasite. This parasite is a major concern for beekeepers and needs specific strategies to reduce its severe impact on honeybees. Here we discuss how helping bees to thrive, especially through changing environments, is of great concern for beekeepers and scientists.
Effect of Sow Body Weight at First Service on Body Status and Performance during First Parity and Lifetime
Authors: Carrión-López María José , Orengo Juan , Madrid Josefa , Vargas Antonio , Martínez-Miró Silvia
Published: December 2022

In this retrospective study, we aimed to assess the effect of body weight (BW) at first service on body status development and sow performance during first parity and lifetime efficiency. A total of 360 DanBred gilts were used, which we categorized into three groups according to their BW: (1) Small sows (<135 kg BW; n = 108); (2) Medium sows (135–150 kg BW; n = 155); (3) Large sows (>150 kg BW; n = 63). We studied the gilts from first insemination to first weaning, and then monitored until culling. Sow body status, litter size and weight, farrowing rate, weaning-to-conception interval, lifetime performance, and hormones linked to metabolism were recorded. Sows in the Small group had the lowest body weight, backfat thickness, and loin depth during gestation. Moreover, they had the smallest number of total piglets born and longest weaning-to-conception interval at first parity. The Small sows also had, on average, one less piglet born during their productive life, and they tended to have a higher culling rate over three parities. For immunoglobulins, insulin, cortisol, and IGF-I levels, no differences were found. In conclusion, achieving optimal body weight at first service is essential for maximizing the sow’s lifetime performance.
Effects of the SLC38A2–mTOR Pathway Involved in Regulating the Different Compositions of Dietary Essential Amino Acids–Lysine and Methionine on Growth and Muscle Quality in Rabbits
Authors: Zhang Bin , Ning Boyuan , Chen Xiaoyang , Li Chenyang , Liu Mengqi , Yue Zhengkai , Liu Lei , Li Fuchang
Published: December 2022

In recent years, ensuring food security has been an important challenge for the world. It is important to make good use of China’s domestic local feed resources to provide safe, stable, efficient, and high-quality rabbit meat products for China and the world. Lysine and methionine are the two most limiting essential amino acids in the rabbit diet. However, little is known about the rational composition of lysine and methionine in rabbit diets and the mechanisms that affect growth and development. Accordingly, in this study, we sought to address this knowledge gap by examining the effects of different compositions of lysine and methionine in rabbit diets. Subsequently, the growth status, nitrogen metabolism, blood biochemical indexes, muscle development, muscle quality, and the growth of satellite cells were evaluated in the animals. The results showed that diets containing 0.80% Lys and 0.40% Met improved average daily weight gain, feed conversion, nitrogen use efficiency, and muscle quality in the rabbits (p < 0.05). Additionally, it altered the amino acid transport potential in muscle by upregulating the expression of the SLC7A10 gene (p < 0.05). Meanwhile, the cell viability and the rate of division and migration of SCs in the 0.80% Lys/0.40 % Met composition group were increased (p < 0.05). SLC38A2 and P–mTOR protein expression was upregulated in the 0.80% lysine/0.40% methionine composition group (p < 0.05). In conclusion, 0.80% Lys/0.40% Met was the most suitable lysine and methionine composition in all tested diets. SLC38A2 acted as an amino acid sensor upstream of mTOR and was involved in the 0.80% Lys/0.40% Met regulation of muscle growth and development, thus implicating the mTOR signaling pathway in these processes.
Concentration of Macroelements and Trace Elements in Farmed Fallow Deer Antlers Depending on Age
Authors: Tajchman Katarzyna , Ukalska-Jaruga Aleksandra , Ceacero Francisco , Pecio Monika , Steiner-Bogdaszewska Żaneta
Published: December 2022

The mineral content of the antlers reflects the nutritional status and specific stage of bone growth in cervid males. Therefore, this research aimed to analyze the concentration of Ca, P, Mg, K, Na, Li, V, Cr, Mn, Co, Cu, Zn, Se and Mo in three characteristic antler positions selected based on the observation of fights between males. These were compared between farmed fallow deer (Dama dama) of different ages. The mineral compositions of tissues were analyzed using inductively coupled plasma mass spectrometry. The highest mean concentrations of macroelements (except K) were recorded in the youngest animals aged 2 or 3 years in the proximal position of the antlers. With age and distance from the skull, Ca, P, Mg and Na contents decreased, while K increased. Higher mean concentrations of most trace elements (Cr, Mn, Co, Cu, Zn) were recorded in 3-year-old animals in antler distal positions. With an increase in the age, body mass and antler mass of fallow deer, the concentration of Ca, P, Mg, K, Mn, Cu and Zn decreased (−0.414 ≤ R ≤ −0.737, p < 0.05) in the studied tissue, whereas Li increased (0.470 ≤ R ≤ 0.681, p < 0.05). The obtained results confirm that the antlers’ chemical composition changes with age, also changing the Ca:P ratio.
Characterization of ddx4 and dnd Homologs in Snakeskin Gourami (Trichopodus pectoralis) and Their Expression Levels during Larval Development and in Gonads of Males and Females
Authors: Nakharuthai Chatsirin , Sreebun Somkiat , Kabpha Apinat , Phuong Tran Vinh , Boonanuntanasarn Surintorn
Published: December 2022

The purpose of this study was to clone and characterize ddx4 and dnd1 homologs in snakeskin gourami (Trichopodus pectoralis) and to determine their expression levels during larval development and in the gonads of males and females. Both cDNAs contained predicted regions that shared consensus motifs with the ddx4 family in teleosts and the dnd family in vertebrates. Phylogenetic tree construction analysis confirmed that these two genes were clustered in the families of teleosts. Both ddx4 and dnd1 mRNAs were detectable only in the gonads, particularly in germ cells. These two genes were expressed during early larval development. The expression of ddx4 was high during early larval development and decreased with increasing developmental age, whereas dnd1 expression increased with developmental age. In adult fish, the expression levels of both genes were higher in the ovary than in the testis. Overall, these findings provide valuable molecular information on ddx4 and dnd, and can be applied in future reproductive biological studies relating to sex dimorphism in snakeskin gourami.
ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen–Thawed Horse Semen
Authors: Dordas-Perpinyà Marta , Yanez-Ortiz Ivan , Sergeant Nicolas , Mevel Vincent , Bruyas Jean-François , Catalán Jaime , Delehedde Maryse , Briand-Amirat Lamia , Miró Jordi
Published: December 2022

ProAKAP4 is the precursor of AKAP4 (A-kinase Anchor protein 4), the main structural protein of the fibrous sheath of sperm. The amount of proAKAP4 reflects the ability of spermatozoa to maintain the flagellum activity and functionality up to the site of fertilization and is positively correlated with progressive motility in several mammalian species. The aim of this study was to investigate the relationship between proAKAP4 concentration with horse sperm motility descriptors and spermatic motile subpopulations. For this purpose, a total of 48 ejaculates from 13 different stallions were analyzed. Spermatic motility descriptors were obtained by the CASA system, and four motile subpopulations (SP) with specific motility patterns were statistically identified. ProAKAP4 concentrations were evaluated by ELISA. The relationship between motility descriptors of sperm subpopulations and proAKAP4 concentrations was evaluated. Following a hierarchical cluster statistical analysis, ejaculates were divided into two groups according to their proAKAP4 concentrations, either having low proAKAP4 concentrations (5.06–35.61 ng/10M spz; n = 23) or high (39.92–82.23 ng/10M spz; n = 25) proAKAP4 concentrations (p < 0.001). ProAKAP4 concentrations were positively correlated (p < 0.05) with total and progressive motility, as well as with parameters of velocity. ProAKAP4 amount also showed a negative correlation (p < 0.05) with sperm motile subpopulation number 3, which was the subpopulation with the lowest velocity parameters. In conclusion, proAKAP4 concentration in stallion semen positively reflects sperm progressive motility with the functional velocity kinematic descriptors. Concentrations of proAKAP4 higher than 37.77 ng/10M spz were correlated with a very good quality frozen/thawed stallion semen.
Neonatology: Topics on Puppies and Kittens Neonatal Management to Improve Neonatal Outcome
Authors: Pereira Keylla Helena Nobre Pacífico , Fuchs Kárita da Mata , Corrêa Jaqueline Valença , Chiacchio Simone Biagio , Lourenço Maria Lucia Gomes
Published: December 2022

The productivity of kennels and catteries is directly linked to maternal prolificacy and neonatal survival. During the neonatal period, mortality is high, not only due to environmental factors after birth but also with regard to gestational fetal life, complications during delivery, and management errors. Neonatology is an area of veterinary medicine where having a strong knowledge base of applied physiology and common clinical presentations for newborns can often favor better outcomes and greater neonatal survival. The teaching of animal theriogenology topics, particularly neonatal medicine, in the veterinary curriculum has evolved significantly in recent years. It is essential that the veterinarian understands the maternal and neonatal particularities and the main aspects involved in the handling of puppies and kittens for the instruction of the correct handling to the breeders and better medical conduct. The breeder of dogs and cats, experienced or not, must count on the help of a veterinarian in their breeding. Proper management, constant monitoring of the litter, and prompt assistance are the keys to successful breeding.
Genetic Relationship between Salmonella Isolates Recovered from Calves and Broilers Chickens in Kafr El-Sheikh City Using ERIC PCR
Authors: Tawfik Rasha Gomaa , Gawish Mahmoud F. , Abotaleb Mahmoud M. , Nada Hassan S. , Morsy Kareem , Abumandour Mohamed M. A. , Torky Helmy
Published: December 2022

A prevalent bacterial intestinal infection with severe economic damage is salmonellosis. Our study was carried out to diagnose Salmonella from chickens and calves, to determine its resistance to antimicrobials’ phenotypic and genotypic characterization of integrons and β lactamase genes in the multidrug resistance of different Salmonella serotypes, and to detect the genetic relationship between Salmonella isolates collected from different origins using an ERIC PCR. In total, 200 samples from diseased chicken and diarrheic calves were obtained from 50 various farms from Kafr El-sheikh, Egypt. Salmonella poultry isolates were characterized as S. Typhimurium (3/8), S. Enteritidis (3/8), and S. Kentucky (2/8), but Salmonella isolates from cattle were S. Enteritidis (1/2) and S. Kentucky (1/2). When antibiotic susceptibility testing was completed on all of the isolates, it showed that there was multidrug resistance present (MDR). A PCR was applied for identifying the accompanying class 1 integrons and ESBLs from MDR Salmonella isolates (two isolates of S. Kentucky were divided as one from calf and one from poultry). Our results detected blaTEM and class 1 integron, but were negative for bla IMP, bla VIM, and bla SHV. An ERIC PCR was conducted for understanding the clonal relation between various β-lactamase-producing MDR Salmonella isolates. The same four previously mentioned isolates were also tested. The two isolates of S. Enteritidis isolated from poultry and calves had 100% similarity despite indicating that there were interactions between broilers and calves living on the same farm that caused infection from the same Salmonella strains, while the other two isolates of S. Kentucky showed only 33% serovarities.
Impact of the COVID-19 Pandemic on the 2020 Diurnal Temperature Range (DTR) in the Contiguous USA
Authors: Ahmed Walid , Hoffmann Lydia Marini , Al-Hasani Talib , Santos Rafael M.
Published: December 2022

Following the emergence of COVID-19, nations around the world implemented effective restrictions that limited people’s movements and economic activity, which reportedly led to environmental improvements. The lowering of air emissions is one environmental indicator that has been connected to the pandemic. The diurnal temperature range (DTR) is one environmental indicator that has been linked to air pollution. In this study, it was hypothesized that because of the pandemic restrictions and slowdowns, the DTR in 2020 for a country that implemented major restrictive measures in reaction to the pandemic would be higher than in previous years, despite or in addition to background climatic forcings. Based on information from weather stations in the contiguous United States of America (USA), the DTR for the year 2020 was compared to the five years before it as a test of this hypothesis. It was verified that the annual mean DTR of 2020 was higher than the three years prior (2017–2019), but lower than the DTR of 2015 and 2016. Compared to historical trends (since 1911), the DTR change in 2020 is within past mean DTR variations that occurred over approx. 12-year cycles, linked to sunspot activity (Schwabe solar cycle). Moreover, climatic effects such as El Niño, La Niña and the prolonged trend of global warming reduce the confidence in the perceived effect of the pandemic. To determine if or how anthropogenic and environmental factors can magnify the impact of the COVID-19 restrictions on the regional mean DTR, five other parameters (annual snowfall quantities, gross domestic product per capita, population density, latitude (northern/southern), and longitude (coastal/inner)) were also examined against changes in DTR from 2015 to 2020. This analysis pointed to the environmental and industrial factors being more strongly correlated with short-term climate changes than societal factors and geographical location.
Improving the Wind Power Density Forecast in the Middle- and High-Latitude Regions of China by Selecting the Relatively Optimal Planetary Boundary Layer Schemes
Authors: Ma Hui , Cao Xin , Ma Xiaolei , Su Haijing , Jing Yanwei , Zhu Kunshuang
Published: December 2022

As the power generation mode with the lowest carbon emissions, wind power generation plays an indispensable role in achieving the goal of carbon neutralization. To optimize the wind power density (WPD), forecasting is crucial to improve wind power utilization and power system stability. However, because near-surface wind is characterized by notable randomness, diversity, intermittence, and uncontrollability, accurately forecasting the WPD on wind farms remains a challenging task. In this study, we attempted to improve the WPD forecast in the middle- and high-latitude regions of China (wind energy resources are abundant there) by selecting the relatively optimal planetary boundary layer (PBL) scheme, as the PBL processes exert notable effects on the near-surface wind field directly. Based on a whole month in the summer (July 2021), seven PBL schemes were compared quantitatively by using the Weather Research and Forecasting (WRF) model for a total of 70 runs (for each run, the forecast period was 3 days). The results show that no PBL schemes could always show the best performance in forecasting all variables, and the forecast accuracy showed a notable dependence on the evolution of the weather systems. Among the seven PBL schemes, the Medium-Range Forecast (MRF) scheme showed the overall best performance in forecasting the 100 m wind speed, sea level pressure, and 2 m temperature, which ensured that it had the highest forecast skill for the WPD in the middle- and high-latitude regions of China. Further analyses indicate that the background conditions were also well forecasted by the MRF scheme (ranked first or second). This was a crucial reason why the WPD forecast was the best for the MRF scheme.
Analysis of Vegetation Coverage Evolution and Degradation under Coal Mine Construction in Permafrost Region
Authors: Wang Shengting , Xu Tianni , Sheng Yu , Wang Yiming , Jia Shuming , Huang Long
Published: December 2022

The ecological environment in permafrost regions is very sensitive to climate change and human activities. The effects of coal mining on the vegetation in permafrost regions have been poorly studied. Herein, on the basis of a field survey in the Juhugen mining area of Qilian Mountain, China, we investigated and quantified the influence of open-pit coal mining on vegetation coverage degradation in permafrost areas. According to the NDVI and field survey, the vegetation coverage was divided into five levels from low to high in the Arc GIS platform. Compared with the area not affected by coal mining, vegetation degradation was significant in the coal-mining-affected area, especially in the high-vegetation-coverage area. The vegetation coverage in Level 5 decreased from 51.99% to 21.35%. According to the conversion matrix, the transfer-out area in high coverage was larger, while the transfer-in area in low vegetation coverage was larger. The transfer-out area of five levels was significant in levels 2–5, accounting for 36.1% to 62.8% of the total area. The transfer-in area of five levels was significant in levels 1–4, accounting for 55.2% to 75.0% of the total area. Moreover, the ground surface temperature and water change were monitored in the vegetation degradation area. The results showed that the above degradation was related to an increase in the ground surface temperature and a decrease in the ground surface moisture.
New Models for Vertical Distribution and Variation of Tropospheric Water Vapor—A Case Study for China
Authors: Wan Moufeng , Zhang Kefei , Wu Suqin , Shen Zhen , Sun Peng , Li Longjiang
Published: December 2022

For better modeling the vertical distribution and variations of water vapor, a 10-year time-series of a newly derived water vapor parameter (termed IRPWV), defined as the ratio of water vapor density (WVD) to the total precipitable water vapor (TPWV), was statistically analyzed. This research showed that the vertical distribution of IRPWV presents a periodic pattern and is highly correlated with the relative magnitude of its corresponding TPWV when compared with the other TPWVs in the same time range. Six TPWV ranges were first chosen to determine the relative magnitude and then used to classify the IRPWV vertical distributions of TPWV. For the periodic variations in each of the six classified IRPWV vertical distribution time-series, a temporal IRPWV model was developed accordingly with six sets of coefficients. The new models were validated by comparing their predictions against the reference values from sounding data at 12 radiosonde stations in China, and their performance was also evaluated against that of the commonly used exponential model. Results showed that, first, the proportions of the height range that had reduced annual root mean square error (RMSE) of WVD in all height ranges within all TPWV ranges were over 75% at the 12 stations. Then, the annual RMSEs of the WVD for all the stations were reduced by at least 11%, 20%, 43%, 48%, 40%, 38%, 32%, 35%, 32%, and 28% in each of the 10 selected height ranges, respectively.
Aluminum/Bromate and Aluminum/Iodate Mechanically Rechargeable Batteries
Authors: Modestov Alexander , Andreev Vladimir , Antipov Anatoliy
Published: December 2022

The ever-increasing characteristics of microcomputers, sensors, actuators, and communication systems require more powerful and more compact autonomous power sources. Al/bromate and Al/iodate flow batteries are proposed as new power supply units for use in oxygen-deficient environments. The batteries employ a mechanically rechargeable aluminum anode flooded with aqueous salt electrolytes or seawater, a cation-exchange membrane, and a carbonaceous porous cathode, where acidified alkali metal bromate, or iodate, is reduced in a six-electron process. The theoretical energy density of an Al/bromate flow cell per reactants is 0.65 kWh kg−1. Seawater is assumed as an electrolyte for the anode compartment. Using a H2/iodate flow cell, it is shown that iodate–iodine–iodide electrochemical transformations can be realized in both directions in acidic media at carbonaceous electrodes. At 30 °C, the area-specific power of the single cells of the Al/bromate and Al/iodate flow batteries reaches 0.26 W cm−2 and 0.075 W cm−2, respectively.
Development of a Rice Plant Disease Classification Model in Big Data Environment
Authors: Sengupta Shampa , Dutta Abhijit , Abdelmohsen Shaimaa A. M. , Alyousef Haifa A. , Rahimi-Gorji Mohammad
Published: December 2022

More than the half of the global population consume rice as their primary energy source. Therefore, this work focused on the development of a prediction model to minimize agricultural loss in the paddy field. Initially, rice plant diseases, along with their images, were captured. Then, a big data framework was used to encounter a large dataset. In this work, at first, feature extraction process is applied on the data and after that feature selection is also applied to obtain the reduced data with important features which is used as the input to the classification model. For the rice disease datasets, features based on color, shape, position, and texture are extracted from the infected rice plant images and a rough set theory-based feature selection method is used for the feature selection job. For the classification task, ensemble classification methods have been implemented in a map reduce framework for the development of the efficient disease prediction model. The results on the collected disease data show the efficiency of the proposed model.
A Review of Brain Activity and EEG-Based Brain–Computer Interfaces for Rehabilitation Application
Authors: Orban Mostafa , Elsamanty Mahmoud , Guo Kai , Zhang Senhao , Yang Hongbo
Published: December 2022

Patients with severe CNS injuries struggle primarily with their sensorimotor function and communication with the outside world. There is an urgent need for advanced neural rehabilitation and intelligent interaction technology to provide help for patients with nerve injuries. Recent studies have established the brain-computer interface (BCI) in order to provide patients with appropriate interaction methods or more intelligent rehabilitation training. This paper reviews the most recent research on brain-computer-interface-based non-invasive rehabilitation systems. Various endogenous and exogenous methods, advantages, limitations, and challenges are discussed and proposed. In addition, the paper discusses the communication between the various brain-computer interface modes used between severely paralyzed and locked patients and the surrounding environment, particularly the brain-computer interaction system utilizing exogenous (induced) EEG signals (such as P300 and SSVEP). This discussion reveals with an examination of the interface for collecting EEG signals, EEG components, and signal postprocessing. Furthermore, the paper describes the development of natural interaction strategies, with a focus on signal acquisition, data processing, pattern recognition algorithms, and control techniques.
Liquid Biopsy in Diagnosis and Prognosis of Non-Metastatic Prostate Cancer
Authors: Rzhevskiy Alexey S. , Kapitannikova Alina Y. , Butnaru Denis V. , Shpot Evgeniy V. , Joosse Simon A. , Zvyagin Andrei V. , Ebrahimi Warkiani Majid
Published: December 2022

Currently, sensitive and specific methods for the detection and prognosis of early stage PCa are lacking. To establish the diagnosis and further identify an appropriate treatment strategy, prostate specific antigen (PSA) blood test followed by tissue biopsy have to be performed. The combination of tests is justified by the lack of a highly sensitive, specific, and safe single test. Tissue biopsy is specific but invasive and may have severe side effects, and therefore is inappropriate for screening of the disease. At the same time, the PSA blood test, which is conventionally used for PCa screening, has low specificity and may be elevated in the case of noncancerous prostate tumors and inflammatory conditions, including benign prostatic hyperplasia and prostatitis. Thus, diverse techniques of liquid biopsy have been investigated to supplement or replace the existing tests of prostate cancer early diagnosis and prognostics. Here, we provide a review on the advances in diagnosis and prognostics of non-metastatic prostate cancer by means of various biomarkers extracted via liquid biopsy, including circulating tumor cells, exosomal miRNAs, and circulating DNAs.
The Gut Microbiome and Its Implication in the Mucosal Digestive Disorders
Authors: Bozomitu Laura , Miron Ingrith , Adam Raileanu Anca , Lupu Ancuta , Paduraru Gabriela , Marcu Florin Mihai , Buga Ana Maria Laura , Rusu Daniela Carmen , Dragan Felicia , Lupu Vasile Valeriu
Published: December 2022

The gastrointestinal (GI) tract is one of the most studied compartments of the human body as it hosts the largest microbial community including trillions of germs. The relationship between the human and its associated flora is complex, as the microbiome plays an important role in nutrition, metabolism and immune function. With a dynamic composition, influenced by many intrinsic and extrinsic factors, there is an equilibrium maintained in the composition of GI microbiota, translated as “eubiosis”. Any disruption of the microbiota leads to the development of different local and systemic diseases. This article reviews the human GI microbiome’s composition and function in healthy individuals as well as its involvement in the pathogenesis of different digestive disorders. It also highlights the possibility to consider flora manipulation a therapeutic option when treating GI diseases.
A Comparative Study of Two Models of Intraluminal Filament Middle Cerebral Artery Occlusion in Rats: Long-Lasting Accumulation of Corticosterone and Interleukins in the Hippocampus and Frontal Cortex in Koizumi Model
Authors: Onufriev Mikhail V. , Stepanichev Mikhail Y. , Moiseeva Yulia V. , Zhanina Marina Y. , Nedogreeva Olga A. , Kostryukov Pavel A. , Lazareva Natalia A. , Gulyaeva Natalia V.
Published: December 2022

Recently, we have shown the differences in the early response of corticosterone and inflammatory cytokines in the hippocampus and frontal cortex (FC) of rats with middle cerebral artery occlusion (MCAO), according to the methods of Longa et al. (LM) and Koizumi et al. (KM) which were used as alternatives in preclinical studies to induce stroke in rodents. In the present study, corticosterone and proinflammatory cytokines were assessed 3 months after MCAO. The most relevant changes detected during the first days after MCAO became even more obvious after 3 months. In particular, the MCAO-KM (but not the MCAO-LM) group showed significant accumulation of corticosterone and IL1β in both the ipsilateral and contralateral hippocampus and FC. An accumulation of TNFα was detected in the ipsilateral hippocampus and FC in the MCAO-KM group. Thus, unlike the MCAO-LM, the MCAO-KM may predispose the hippocampus and FC of rats to long-lasting bilateral corticosterone-dependent distant neuroinflammatory damage. Unexpectedly, only the MCAO-LM rats demonstrated some memory deficit in a one-trial step-through passive avoidance test. The differences between the two MCAO models, particularly associated with the long-lasting increase in glucocorticoid and proinflammatory cytokine accumulation in the limbic structures in the MCAO-KM, should be considered in the planning of preclinical experiments, and the interpretation and translation of received results.
GARP Regulates the Immune Capacity of a Human Autologous Platelet Concentrate
Authors: Trzeciak Emily R. , Zimmer Niklas , Kämmerer Peer W. , Thiem Daniel , Al-Nawas Bilal , Tuettenberg Andrea , Blatt Sebastian
Published: December 2022

Autologous platelet concentrates, like liquid platelet rich fibrin (iPRF), optimize wound healing; however, the underlying immunological mechanisms are poorly understood. Platelets, the main cellular component of iPRF, highly express the protein, Glycoprotein A repetitions predominant (GARP), on their surfaces. GARP plays a crucial role in maintaining peripheral tolerance, but its influence on the immune capacity of iPRF remains unclear. This study analyzed the interaction of iPRF with immune cells implicated in the wound healing process (human monocyte derived macrophages and CD4+ T cells) and evaluated the distinct influence of GARP on these mechanisms in vitro. GARP was determined to be expressed on the surface of platelets and to exist as a soluble factor in iPRF. Platelets derived from iPRF and iPRF itself induced a regulatory phenotype in CD4+ T cells, shown by increased expression of Foxp3 and GARP as well as decreased production of IL-2 and IFN-γ. Application of an anti-GARP antibody reversed these effects. Additionally, iPRF polarized macrophages to a “M0/M2-like” phenotype in a GARP independent manner. Altogether, this study demonstrated for the first time that the immune capacity of iPRF is mediated in part by GARP and its ability to induce regulatory CD4+ T cells.
A Novel Improved Thromboembolism-Based Rat Stroke Model That Meets the Latest Standards in Preclinical Studies
Authors: Pawletko Katarzyna , Jędrzejowska-Szypułka Halina , Bogus Katarzyna , Pascale Alessia , Fahmideh Foroogh , Marchesi Nicoletta , Grajoszek Aniela , Olakowska Edyta , Barski Jarosław Jerzy
Published: December 2022

The animal thromboembolic model of ischemia perfectly mimics human ischemic stroke which remains the leading cause of disability and mortality in humans. The development of new treatment strategies was therefore imperative. The purpose of this study is to improve the thromboembolic stroke model in rats in order to design experiments that use motor tests, and are in accordance with the 3R principles to prevent complications and maintain the same size of the infarct repeatedly. Tail vein dye application, a protective skull mask and a stress minimization protocol were used as additional modifications to the animal stroke model. These modifications significantly minimized the pain and stress severity of the procedures in this model. In our experimental group of Long-Evans rats, a photo-induced stroke was caused by the application of a photosensitive dye (Rose Bengal) activated with white-light irradiation, thus eliminating the need to perform a craniotomy. The animals’ neurological status was evaluated using a runway elevated test. Histological examination of the brain tissue was performed at 12, 24 and 48 h, and seven days post-stroke. Tissue examination revealed necrotic foci in the cortex and the subcortical regions of the ipsilateral hemisphere in all experimental groups. Changes in the area, width and depth of the necrotic focus were observed over time. All the experimental groups showed motor disturbances after stroke survival. In the proposed model, photochemically-induced stroke caused long-term motor deficits, showed high reproducibility and low mortality rates. Consequently, the animals could participate in motor tests which are particularly suitable for assessing the efficacy of neuro-regenerative therapies, while remaining in line with the latest trends in animal experimental design.
Experimental and Numerical Study on Steel Fiber Concrete under Blast Loading
Authors: Yin Huawei , Ouyang Yaoguo
Published: December 2022

In this paper, 30 SFRC (Steel Fiber Reinforced Concrete) spindle specimens with different steel fiber contents were subjected to static loading tests and blast wave loading tests on spindle specimens with different steel fiber contents using a self-developed planar blast wave loading device (a new type of patent recognized by the State Intellectual Property Rights). The dynamic response, impact performance and damage mode of Steel Fiber Reinforced Concrete under blast loading were investigated. The experimental results show that with the increase of steel fiber content (within 2%), the strength of the Steel Fiber Reinforced Concrete increases slightly. The flatter the falling section of the stress-strain curve, the better the energy absorption effect. With the increase of explosive equivalent(24 g RDX and 36 g RDX), the more obvious the strain rate effect, the greater the increase of peak stress, and the SFRC with 2% steel fiber content has the best energy absorption effect. Furthermore, the dynamic response of SFRC spindle specimens was numerically simulated using the improved K&C material model with LS-DYNA explicit finite element dynamic analysis software. The results verify the validity and reliability of the improved K&C material model.
Carbon Nanomaterials-Based Electrically Conductive Scaffolds to Repair the Ischaemic Heart Tissue
Authors: Ul Haq Arsalan , Carotenuto Felicia , Trovalusci Federica , De Matteis Fabio , Di Nardo Paolo
Published: December 2022

Ischaemic heart diseases are the leading causes of morbidity around the world and pose serious socio-economic burdens. Ischaemic events, such as myocardial infarction, lead to severe tissue damage and result in the formation of scar tissue. This scar tissue, being electrically inert, does not conduct electrical currents and thus generates lethal arrhythmias. The ventricle dilates with time due to asynchronous beating due to the scar, and it eventually leads to total heart failure. The current pharmacological approaches only cure heart failure symptoms without inducing tissue regeneration. Therefore, heart transplant remains the gold standard to date, but the limited organ donors and the possibility of immune rejection make this approach elusive. Cardiac tissue engineering has the potential to address this issue by engineering artificial heart tissues using 3D scaffolds cultured with cardiac stem cells. Compared with the traditional non-conductive scaffold, electroconductive scaffolds can transfer feeble electric currents among the cultured cells by acting as a “wire”. This improves intercellular communication and synchronisation that otherwise is not possible using non-conductive scaffolds. This article reviews the recent advances in carbon nanomaterials-based electroconductive scaffolds, their in vitro/in vivo efficacy, and their potential to repair ischaemic heart tissue.
Development of the Correlation Model between Biogas Yield and Types of Organic Mass and Analysis of Its Key Factors
Authors: Mirzoieva Tetiana , Tkach Nazar , Nitsenko Vitalii , Gerasymchuk Nataliia , Tomashevska Olga , Nechyporenko Oleksandr
Published: December 2022

Since European society is experiencing an aggravation of the issue of energy security, the production of renewable energy is becoming increasingly important. The advantages of biofuel—in particular, biogas—and the positive effects of the development of its production are summarized within the framework of the problem statement. It is emphasized that the production of biogas from various renewable raw materials causes economic, ecological, and social effects. The development of biogas production can be especially active in combination with the development of the agricultural sphere. In response to today’s demand, the authors in this research present a model of the correlation between the output of biogas from different types of organic mass and specify the factors affecting it. In particular, a multiple econometric model of the relationship between the output of biogas from different types of organic mass and the content of dry organic matter and the share of possible methane content in organic matter was built; the density of the connection between the factors and the resulting feature was evaluated; the tightness of the general relationship (influence) of independent variables on the dependent variable was checked using the coefficient of determination; and the reliability of the correlation characteristics was estimated using Fisher’s and Student’s tests. As a result, with the use of convincing evidence—in particular, taking into account the potential of the Ukrainian agricultural sector—the feasibility of further development of biogas production in combination with the development of agricultural production is substantiated.
Clinical Outcomes in COVID-19 Patients Treated with Immunotherapy
Authors: Hatic Haris , Hearld Kristine R. , Das Devika , Deshane Jessy
Published: December 2022

Introduction: The full impact of COVID-19 infections on patients with cancer who are actively being treated with chemotherapy or immune checkpoint inhibitors (ICIs) has not been fully defined. Our goal was to track clinical outcomes in this specific patient population. Methods: We performed a retrospective chart review of 121 patients (age > 18 years) at the University of Alabama at Birmingham from January 2020 to December 2021 with an advanced solid malignancy that were eligible to be treated with ICIs or on current therapy within 12 months of their COVID-19 diagnosis. Results: A total of 121 patients were examined in this study, and 61 (50.4%) received immunotherapy treatment within 12 months. One quarter of the patients on ICIs passed away, compared to 13% of the post-chemotherapy cohort. Patients who were vaccinated for COVID-19 had lower mortality compared to unvaccinated patients (X2 = 15.19, p < 0.001), and patients with lower ECOG (0.98) were associated with lower mortality compared to patients with worse functional status (0.98 vs. 1.52; t = 3.20; p < 0.01). Conclusions: COVID-19-related ICI mortality was higher compared to patients receiving chemotherapy. However, ICI cessation or delay is unwarranted as long there has been a risk–benefit assessment undertaken with the patient.
The Dual Role of PDCD10 in Cancers: A Promising Therapeutic Target
Authors: Liu Jingdian , Zhao Kai , Wu Sisi , Li Chaoxi , You Chao , Wang Junwen , Shu Kai , Lei Ting
Published: December 2022

Programmed cell death 10 (PDCD10) was initially considered as a protein associated with apoptosis. However, recent studies showed that PDCD10 is actually an adaptor protein. By interacting with multiple molecules, PDCD10 participates in various physiological processes, such as cell survival, migration, cell differentiation, vesicle trafficking, cellular senescence, neurovascular development, and gonadogenesis. Moreover, over the past few decades, accumulating evidence has demonstrated that the aberrant expression or mutation of PDCD10 is extremely common in various pathological processes, especially in cancers. The dysfunction of PDCD10 has been strongly implicated in oncogenesis and tumor progression. However, the updated data seem to indicate that PDCD10 has a dual role (either pro- or anti-tumor effects) in various cancer types, depending on cell/tissue specificity with different cellular interactors. In this review, we aimed to summarize the knowledge of the dual role of PDCD10 in cancers with a special focus on its cellular function and potential molecular mechanism. With these efforts, we hoped to provide new insight into the future development and application of PDCD10 as a clinical therapeutic target in cancers.
Magnetic Resonance Spectroscopy Metabolites as Biomarkers of Disease Status in Pediatric Diffuse Intrinsic Pontine Gliomas (DIPG) Treated with Glioma-Associated Antigen Peptide Vaccines
Authors: Panigrahy Ashok , Jakacki Regina I. , Pollack Ian F. , Ceschin Rafael , Okada Hideho , Nelson Marvin D. , Kohanbash Gary , Dhall Girish , Bluml Stefan
Published: December 2022

Purpose: Diffuse intrinsic pontine gliomas (DIPG) are highly aggressive tumors with no currently available curative therapy. This study evaluated whether measurements of in vivo cell metabolites using magnetic resonance spectroscopy (MRS) may serve as biomarkers of response to therapy, including progression. Methods: Single-voxel MR spectra were serially acquired in two cohorts of patients with DIPG treated with radiation therapy (RT) with or without concurrent chemotherapy and prior to progression: 14 participants were enrolled in a clinical trial of adjuvant glioma-associated antigen peptide vaccines and 32 patients were enrolled who did not receive adjuvant vaccine therapy. Spearman correlations measured overall survival associations with absolute metabolite concentrations of myo-inositol (mI), creatine (Cr), and n-acetyl-aspartate (NAA) and their ratios relative to choline (Cho) during three specified time periods following completion of RT. Linear mixed-effects regression models evaluated the longitudinal associations between metabolite ratios and time from death (terminal decline). Results: Overall survival was not associated with metabolite ratios obtained shortly after RT (1.9–3.8 months post-diagnosis) in either cohort. In the vaccine cohort, an elevated mI/Cho ratio after 2–3 doses (3.9–5.2 months post-diagnosis) was associated with longer survival (rho = 0.92, 95% CI 0.67–0.98). Scans performed up to 6 months before death showed a terminal decline in the mI/Cho ratio, with an average of 0.37 ratio/month in vaccine patients (95% CI 0.11–0.63) and 0.26 (0.04–0.48) in the non-vaccine cohort. Conclusion: Higher mI/Cho ratios following RT, consistent with less proliferate tumors and decreased cell turnover, were associated with longer survival, suggesting that this ratio can serve as a biomarker of prognosis following RT. This finding was seen in both cohorts, although the association with OS was detected earlier in the vaccine cohort. Increased mI/Cho (possibly reflecting immune-effector cell influx into the tumor as a mechanism of tumor response) requires further study.