bluuebunny/crossref_metadata_2025 huggingface dataset:
Systemic Arterial Function after Multisystem Inflammatory Syndrome in Children Associated with COVID-19
Authors: Mukhopadhyay Ketaki , Johnston Marla S. , Krulisky James S. , Yang Shengping , Kimball Thomas R.
Published: July 2024

Introduction: Multisystem inflammatory syndrome in children (MIS-C) is a new disease entity occurring in the pediatric population two to six weeks after coronavirus exposure due to a systemic arteritis. We investigated post-hospital-discharge arterial function at short- and mid-term intervals using pulse wave velocity. We assessed associations between arterial function, left ventricular diastolic and systolic function and left ventricular mass. Materials and methods: Retrospective data collection was carried out on 28 patients with MIS-C with at least two outpatient pediatric cardiology clinic visits post hospital admission. The patients underwent assessment of systemic arterial function and cardiac function. Data included pulse wave velocity between carotid and femoral arteries and echocardiographic assessment of left ventricular systolic function (shortening and ejection fraction, longitudinal strain), diastolic function and left ventricular mass. Results: Pulse wave velocity significantly decreased from visit 1 to visit 2 (5.29 ± 1.34 m/s vs. 4.51 ± 0.91 m/s, p = 0.009). Left ventricular mass significantly decreased from visit 1 to visit 2 (42 ± 9 g/m2.7 vs. 38 ± 7 g/m2.7, p = 0.02). There was a significant negative correlation between the pulse wave velocity and E/A mitral inflow (−0.41, p < 0.05). Conclusions: Children have elevated pulse wave velocity and left ventricular mass in the short-term relative to mid-term values after hospital discharge. These results suggest that MIS-C is associated with transient systemic arterial dysfunction, which, in turn, may play a role in cardiac changes.
Grassland Ecosystem Services: Their Economic Evaluation through a Systematic Review
Authors: Pergola Maria , De Falco Enrica , Cerrato Michele
Published: July 2024

Grasslands provide a wide range of provision, support, regulation, and cultural ecosystem services (ESs), whose valuation methods can be grouped into three categories (ecological, sociocultural, and economic). The present manuscript aims to provide an overview of academic studies on grassland ESs and of the most used economic evaluation methods. To this end, a systematic and bibliometric review was conducted using the scientific database Scopus and the VOSviewer software. The results highlighted that China and the USA were the main countries with the highest number of publications regarding ESs provided by grasslands. The number of publications began to grow starting in 2005, thanks, perhaps, to the publication of influential documents, such as the Millennium Ecosystem Assessment, and the general increase in interest in ecological policy issues. The year 2023 had the highest number of documents in absolute (646), demonstrating the timeliness, importance, and relevance of this research topic. The most studied grassland ES has been carbon storage; however, a central role was played also by biodiversity. In this context, papers that estimated grassland ESs from an economic perspective represented only 3% of all papers that Scopus has returned. More than half of these referred to the use of equivalent coefficients to calculate the ES value of different land uses/land cover categories or, at most, of 11 types of ES. All this highlights the difficulty in estimating individual ESs provided by grasslands from an economic point of view and the greater propensity to use physical, chemical, and biological indicators. Consequently, the sustainable management of grasslands requires more studies on the economic evaluation of their ES, as well as environmental aspects in the economic accounting of governments, or to implement a support system for farms in delivering various ecosystem services.
Per Capita Land Use through Time and Space: A New Database for (Pre)Historic Land-Use Reconstructions
Authors: Hill Chad , Madella Marco , Whitehouse Nicki J. , Jiménez-Arteaga Carolina , Hammer Emily , Bates Jennifer , Welton Lynn , Biagetti Stefano , Hilpert Johanna , Morrison Kathleen D.
Published: July 2024

Anthropogenic land cover change (ALCC) models, commonly used for climate modeling, tend to utilize relatively simplistic models of human interaction with the environment. They have historically relied on unsophisticated assumptions about the temporal and spatial variability of the area needed to support one person: per capita land use (PCLU). To help refine ALCC models, we used a range of data sources to build a new database that attempts to bring together PCLU data with significant time depth and a global perspective. This new database can provide new nuance for our understanding of the variability in land use among and between time periods and regions, data that will have wide applicability for continued research into past human land use and present land-use change, and can hopefully help improve existing ALCC models. An example is provided, showing the potential impact of new PCLU data on land-use mapping in the Middle East at 6000 BP.
Predicting Land-Use Change Trends and Habitat Quality in the Tarim River Basin: A Perspective with Climate Change Scenarios and Multiple Scales
Authors: Aishan Tayierjiang , Song Jian , Halik Ümüt , Betz Florian , Yusup Asadilla
Published: July 2024

Under the influences of climate change and human activities, habitat quality (HQ) in inland river basins continues to decline. Studying the spatiotemporal distributions of land use and HQ can provide support for sustainable development strategies of the ecological environment in arid regions. Therefore, this study utilized the SD-PLUS model, InVEST-HQ model, and Geodetector to assess and simulate the land-use changes and HQ in the Tarim River Basin (TRB) at multiple scales (county and grid scales) and scenarios (SSP126, SSP245, and SSP585). The results indicated that (1) the Figure of Merit (FoM) values for Globeland 30, China’s 30 m annual land-cover product, and the Chinese Academy of Sciences (30 m) product were 0.22, 0.12, and 0.15, respectively. A comparison of land-use datasets with different resolutions revealed that the kappa value tended to decline as the resolution decreased. (2) In 2000, 2010, and 2020, the HQ values were 0.4656, 0.4646, and 0.5143, respectively. Under the SSP126 and SSP245 scenarios, the HQ values showed an increasing trend: for the years 2030, 2040, and 2050, they were 0.4797, 0.4834, and 0.4855 and 0.4805, 0.4861, and 0.4924, respectively. Under SSP585, the HQ values first increased and then decreased, with values of 0.4791, 0.4800, and 0.4766 for 2030, 2040, and 2050, respectively. (3) Under three scenarios, areas with improved HQ were mainly located in the southern and northern high mountain regions and around urban areas, while areas with diminished HQ were primarily in the western part of the basin and central urban areas. (4) At the county scale, the spatial correlation was not significant, with Moran’s I ranging between 0.07 and 0.12, except in 2000 and 2020. At the grid scale, the spatial correlation was significant, with clear high- and low-value clustering (Moran’s I between 0.80 and 0.83). This study will assist land-use planners and policymakers in formulating sustainable development policies to promote ecological civilization in the basin.
Optimizing Territorial Spatial Structures within the Framework of Carbon Neutrality: A Case Study of Wuan
Authors: Han Xiangxue , Fu Meichen , Wang Jingheng , Li Sijia
Published: July 2024

Climate change has become a major worldwide problem, and land use/cover change has consistently played a crucial role in impacting the carbon cycle within terrestrial ecosystems. Territorial spatial planning stands as a relatively good policy option for the low-carbon model. The spatial correlation between carbon emissions and land use was established through environmental parameters in this paper. The territorial spatial structures in 2035 and 2060 under two scenarios of natural evolution and low-carbon development were simulated through the PLUS model. The results indicate that the spatial pattern of decreasing carbon emissions centered on towns, cities, mines, and industries is related to regional economic development, the distribution of forests, and the urban ecological environment. The implementation of territorial spatial planning aids in achieving carbon neutrality, whereas the low-carbon development scenario is more focused on it, which can provide ideas for territorial spatial planning adjustments. Both scenarios result in a large area of fallow land, indicating some conflict between farmland protection and low-carbon development. Optimizing management measures, energy structure, and industrial layout and strengthening regional coordination are key to promoting low-carbon development. This study might be useful in formulating regional carbon-neutral policies and improving territorial spatial planning.
Advanced Materials for Biomedical Applications, Editorial Article
Authors: Peralta-Rodríguez René D. , Mendoza-Mendoza Esmeralda , Liakos Ioannis L.
Published: July 2024

Advanced materials (AMs) encompass materials that feature improved properties compared to common counterparts [...]
Fatigue Strength Improvement of Laser-Directed Energy Deposition 316L Stainless Steel with In Situ Ultrasonic Rolling by Preliminary Investigation
Authors: Liu Guan , Su Yigui , Pi Xuyu , Liu Defu , Lin Yongcheng
Published: July 2024

In this study, to improve the fatigue strength of the LDED (laser-directed energy deposition) 316L stainless steel, an in situ ultrasonic rolling technology is developed to assist the laser-directed energy deposition process (LDED-UR). The microstructural characteristics and fatigue behavior are comprehensively discussed. The results show that the average size of pores of the LDED-UR alloy is about 10.2 μm, which is much smaller than that of the LDED alloy (34.1 μm). Meanwhile, the density of the LDED alloy is also enhanced from 98.26% to 99.27% via the in situ ultrasonic rolling. With the application of the in situ ultrasonic rolling, the grains are transformed into fully equiaxed grains, and their average grain size is greatly reduced from 84.56 μm to 26.93 μm. The fatigue limit of the LDED-UR alloy is increased by 29% from 210 MPa (LDED alloy) to 270 MPa, which can be ascribed to the decreased porosity and the fine grains. In particular, the crack initiation site of the LDED alloy is located at the surfaces, while it is nucleated from the sub-surface for the LDED-UR alloy. This is mainly attributed to the compression residual stress induced by the in situ ultrasonic rolling. This research offers a valuable understanding of the failure mechanisms in additively manufactured metals, guiding the development of effective strategies to improve their fatigue threshold under severe operating conditions.
Numerical Investigation of the Performance of a Proton Exchange Membrane Water Electrolyzer under Various Outlet Manifold Structure Conditions
Authors: Zhang Guobin , Qu Zhiguo
Published: July 2024

The oxygen discharge process significantly affects the electrochemical performance of a proton exchange membrane water electrolyzer (PEMWE), which requires an optimal structure of the flow field implemented in the bipolar plate (BP) component. In this study, we numerically investigated the two-phase (liquid water and oxygen) flow in the PEMWE’s channel region with different outlet manifold structures utilizing the volume of fluid (VOF) model. Then, the oxygen volume fraction at the liquid/gas diffusion layer (L/GDL) surface, i.e., the interface of the channel and L/GDL, obtained by the liquid water and oxygen flow model was incorporated into a three-dimensional (3D) PEMWE model, which made it possible to predict the influence of the outlet manifold structure on the multiple transfers inside the whole electrolyzer as well as the electrochemical performance. The results indicate that the existence of oxygen in the flow field significantly decreased the electrolyzer voltage at a fixed operation current density and deteriorated the uniform distribution of the oxygen amount, current density (corresponding to the electrochemical reaction rate) and temperature in the membrane electrode assembly (MEA), indicating that the rapid oxygen removal from the flow field is preferred in the operation of the electrolyzer. Moreover, slight increases in the width of the outlet manifold were helpful in relieving the oxygen accumulation in the anode CL and, hence, improved the electrolyzer performance with more uniform distribution characteristics.
State Space Representation of Jiles–Atherton Hysteresis Model and Application for Closed-Loop Control
Authors: Zhao Jiye , Zhou Jiqiang , Zhang Lu , Sun Jinji
Published: July 2024

Hysteresis is a fundamental characteristic of magnetic materials. The Jiles–Atherton (J-A) hysteresis model, which is known for its few parameters and clear physical interpretations, has been widely employed in simulating hysteresis characteristics. To better analyze and compute hysteresis behavior, this study established a state space representation based on the primitive J-A model. First, based on the five fundamental equations of the J-A model, a state space representation was established through variable substitution and simplification. Furthermore, to address the singularity problem at zero crossings, local linearization was obtained through an approximation method based on the actual physical properties. Based on these, the state space model was implemented using the S-function. To validate the effectiveness of the state space model, the hysteresis loops were obtained through COMSOL finite element software and tested on a permalloy toroidal sample. The particle swarm optimization (PSO) method was used for parameter identification of the state space model, and the identification results show excellent agreement with the simulation and test results. Finally, a closed-loop control system was constructed based on the state space model, and trajectory tracking experiments were conducted. The results verify the feasibility of the state space representation of the J-A model, which holds significant practical implications in the development of magnetically shielded rooms, the suppression of magnetic interference in cold atom clocks, and various other applications.
Increasing the Strength and Impact Toughness of Carbon Steel Using a Nanosized Eutectoid Resulting from Time-Controlled Quenching
Authors: Brykov Michail , Mierzwiński Dariusz , Efremenko Vasily , Girzhon Vasyl’ , Shalomeev Vadim , Shyrokov Oleksandr V. , Petryshynets Ivan , Klymov Olexandr , Kapustyan Oleksii
Published: July 2024

High-carbon steels are normally used as tool materials. The use of such steels for construction is limited due to their increased brittleness and poor weldability. However, it appears that high-carbon steels possess certain hidden reserves for enhanced plasticity and strength if properly heat-treated. An unconventional heat treatment was applied to carbon eutectoid steel (0.8 wt.% C) in order to increase its strength and impact toughness simultaneously. Samples for tensile and impact testing were held at 800 °C for different time ranges from 3 min to 9 min with subsequent cooling in oil. It was established that for each type of sample, an optimal holding time exists that is responsible for increased strength and high impact toughness. The hardness and microhardness levels of the surface and under-surface regions of the samples reached 390 HV after optimal heat treatment. An X-ray revealed a shift of the (211)α-peak to the lower 2-theta angles after heat treatment with the optimal holding time; this indicates an increase in carbon content in alpha solid solutions of approximately 0.12 wt.%. Thus, a nanostructured mixture of low-carbon martensite and thin cementite plates is formed in the under-surface region of carbon eutectoid steel after heat treatment, with a controlled holding time at the austenitizing temperature.
Polymer Materials for Optoelectronics and Energy Applications
Authors: Lim Ju Won
Published: July 2024

This review comprehensively addresses the developments and applications of polymer materials in optoelectronics. Especially, this review introduces how the materials absorb, emit, and transfer charges, including the exciton–vibrational coupling, nonradiative and radiative processes, Förster Resonance Energy Transfer (FRET), and energy dynamics. Furthermore, it outlines charge trapping and recombination in the materials and draws the corresponding practical implications. The following section focuses on the practical application of organic materials in optoelectronics devices and highlights the detailed structure, operational principle, and performance metrics of organic photovoltaic cells (OPVs), organic light-emitting diodes (OLEDs), organic photodetectors, and organic transistors in detail. Finally, this study underscores the transformative impact of organic materials on the evolution of optoelectronics, providing a comprehensive understanding of their properties, mechanisms, and diverse applications that contribute to advancing innovative technologies in the field.
The Mechanical and Electrochemical Stability of Trimethysilane Plasma Nanocoatings Deposited onto Cobalt Chromium Cardiovascular Stents
Authors: Phan ThiThuHa , Jones John E. , Liao Yixuan , Yu Qingsong , Chen Meng
Published: July 2024

The objective of this study was to evaluate the coating integrity performance and corrosion protection property of trimethylsilane (TMS) plasma nanocoatings that were directly deposited onto cobalt chromium (CoCr) L605 cardiovascular stents. Hydrophilic surfaces were achieved for the TMS plasma nanocoatings that were deposited onto the coronary stents through NH3/O2 (2:1 molar ratio) plasma post-treatment. With a coating thickness of approximately 20–25 nm, the TMS plasma nanocoatings were highly durable and able to resist delamination and cracking from crimping and expansion by a Model CX with a J-Crimp Station. The stent surface that was evaluated by Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectroscopy (EDS) showed no indications of pitting, corrosion, or adsorption products on either the luminal or abluminal surfaces of the stents, in contrast to the uncoated stent surface. The TMS plasma nanocoatings significantly enhanced the stent’s corrosion resistance in immersion experiments that followed the ASTM F2129-15 corrosion protocol, evident in the increase of the open circuit potential (OCP) from 0.01 V for the uncoated L605 stent to 0.18 V for the plasma-nanocoated L605 stent, reducing potential cytotoxic metal ion release. Cyclic polarization (CP) curves show that the corrosion rate (density level) observed in plasma-nanocoated L605 stents was approximately half an order of magnitude lower than that of the uncoated stents, indicating improved corrosion protection of the stents. CP curves of the TMS plasma-nanocoated stents with different coating thicknesses show that, in the range of 20–65 nm, the coating thickness does not result in any difference in the corrosion resistance of the stents.
Multi-Step Procedure for Predicting Early-Age Thermal Cracking Risk in Mass Concrete Structures
Authors: Klemczak Barbara , Smolana Aneta
Published: July 2024

Early-age cracking in mass concrete structures resulting from thermal stress is a well-documented phenomenon that impacts their functionality, durability, and integrity. The primary cause of these cracks is the uneven temperature rise within the structure due to the exothermic nature of cement hydration. Assessing the likelihood of cracking involves comparing the tensile strength or strain capacity of the concrete with the stresses or strains experienced by the structure. Challenges in evaluating the risk of thermal cracking in mass concrete structures stem from various material and technological factors that influence the magnitude and progression of hydration heat-induced temperature and thermal stress. These complexities can be addressed through numerical analysis, particularly finite element analysis (FEA), which offers comprehensive modeling of early-age effects by considering all pertinent material and technological variables. However, employing FEA poses challenges such as the requirement for numerous input parameters, which may be challenging to define, and the need for specialized software not commonly available to structural engineers. Consequently, the necessity for such advanced modeling, which demands significant time investment, may not always be warranted and should be initially assessed through simpler methods. This is primarily because the definition of massive structures—those susceptible to adverse effects such as cracking due to temperature rise from hydration heat—is not precise. To address these challenges, the authors propose a three-step method for evaluating structures in this regard. The first step involves a simplified method for the classification of massive structures. The second step entails estimating hardening temperatures and levels of thermal stress using straightforward analytical techniques. The third step, reserved for structures identified as having a potential risk of early thermal cracks, involves numerical modeling. The outlined procedure is illustrated with an example application, demonstrating its practicality in analyzing a massive concrete wall constructed on the foundation.
Identification Tools of Microplastics from Surface Water Integrating Digital Image Processing and Statistical Techniques
Authors: Dacewicz Ewa , Łobos-Moysa Ewa , Chmielowski Krzysztof
Published: July 2024

The primary objective of this study was to demonstrate the potential of digital image analysis as a tool to identify microplastic (MP) particles in surface waters and to facilitate their characterisation in terms of 2D and 3D morphology. Digital image analysis preceded by microscopic analysis was used for an exhaustive quantitative and qualitative evaluation of MPs isolated from the Vistula River. Using image processing procedures, 2D and 3D shape descriptors were determined. Principal Component Analysis was used to interpret the relationships between the parameters studied, characterising MP particle geometry, type and colour. This multivariate analysis of the data allowed three or four main factors to be extracted, explaining approximately 90% of the variation in the data characterising MP morphology. It was found that the first principal component for granules, flakes and films was largely represented by strongly correlated with 2D shape descriptors (area, perimeter, equivalent area diameter) and 3D shape descriptors (Corey Shape Factor, Compactness, Dimensionality). Considering the scraps, principal component PC1 was represented by only five of the above descriptors, and the Compactness variable had the largest contribution to principal component PC2. In addition, for granules, flakes and films, a relationship between 2D shape and the colour of their particles could be observed. For the most numerous MP group identified of multicoloured scraps, no such association was found. The results of our study can be used for further multivariate analysis regarding the presence of microplastic floating on the river surface, with a particular focus on particles of secondary origin. This is of key importance for optimising future efforts in conducting small-scale and multidimensional monitoring of and reducing plastics in the aquatic environment.
Increased Cytotoxicity of Bimetallic Ultrasmall Silver–Platinum Nanoparticles (2 nm) on Cells and Bacteria in Comparison to Silver Nanoparticles of the Same Size
Authors: Wolff Natalie , Białas Nataniel , Loza Kateryna , Heggen Marc , Schaller Torsten , Niemeyer Felix , Weidenthaler Claudia , Beuck Christine , Bayer Peter , Prymak Oleg , Oliveira Cristiano L. P. , Epple Matthias
Published: July 2024

Ultrasmall nanoparticles (diameter 2 nm) of silver, platinum, and bimetallic nanoparticles (molar ratio of Ag:Pt 0:100; 20:80; 50:50; 70:30; 100:0), stabilized by the thiolated ligand glutathione, were prepared and characterized by transmission electron microscopy, differential centrifugal sedimentation, X-ray photoelectron spectroscopy, small-angle X-ray scattering, X-ray powder diffraction, and NMR spectroscopy in aqueous dispersion. Gold nanoparticles of the same size were prepared as control. The particles were fluorescently labeled by conjugation of the dye AlexaFluor-647 via copper-catalyzed azide-alkyne cycloaddition after converting amine groups of glutathione into azide groups. All nanoparticles were well taken up by HeLa cells. The cytotoxicity was assessed with an MTT test on HeLa cells and minimal inhibitory concentration (MIC) tests on the bacteria Escherichia coli and Staphylococcus xylosus. Notably, bimetallic AgPt nanoparticles had a higher cytotoxicity against cells and bacteria than monometallic silver nanoparticles or a physical mixture of silver and platinum nanoparticles. However, the measured release of silver ions from monometallic and bimetallic silver nanoparticles in water was very low despite the ultrasmall size and the associated high specific surface area. This is probably due to the surface protection by a dense layer of thiolated ligand glutathione. Thus, the enhanced cytotoxicity of bimetallic AgPt nanoparticles is caused by the biological environment in cell culture media, together with a polarization of silver by platinum.
Modeling and Control of the High-Voltage Terminal of a Tandem Van de Graaff Accelerator
Authors: Gutiérrez Ocampo Efrén , Saldivar Belem , Ávila Vilchis Juan Carlos , Portillo-Rodríguez Otniel
Published: July 2024

This paper presents a mathematical model derived from an equivalent electrical circuit to describe the dynamic behavior of the high-voltage terminal of a tandem Van de Graaff accelerator. Two approaches are presented for modeling the transit time of the current flowing through the corona needles. The first one considers an equivalent self-inductance in the corona triode, whereas, in the second one, the transit time is represented by a delay in the corona current. The validation of the proposed models was carried out through experimental tests developed at the National Nuclear Research Institute of Mexico. Furthermore, two strategies for controlling the slow variations of the terminal voltage limited by the slow response of the control loop based on corona discharge are evaluated: a Proportional–Integral–Derivative controller and a sliding mode controller. The Root-Mean-Squared Error calculation leads to the conclusion that both control strategies are suitable for regulating the voltage at the accelerator potential terminal. However, the sliding mode controller leads to an overshoot-free response and a shorter settling time.
Time Series Forecasting with Many Predictors
Authors: Huang Shuo-Chieh , Tsay Ruey S.
Published: July 2024

We propose a novel approach for time series forecasting with many predictors, referred to as the GO-sdPCA, in this paper. The approach employs a variable selection method known as the group orthogonal greedy algorithm and the high-dimensional Akaike information criterion to mitigate the impact of irrelevant predictors. Moreover, a novel technique, called peeling, is used to boost the variable selection procedure so that many factor-relevant predictors can be included in prediction. Finally, the supervised dynamic principal component analysis (sdPCA) method is adopted to account for the dynamic information in factor recovery. In simulation studies, we found that the proposed method adapts well to unknown degrees of sparsity and factor strength, which results in good performance, even when the number of relevant predictors is large compared to the sample size. Applying to economic and environmental studies, the proposed method consistently performs well compared to some commonly used benchmarks in one-step-ahead out-sample forecasts.
Some Properties and Graphical Applications of a New Subclass of Harmonic Functions Defined by a Differential Inequality
Authors: Yalçın Sibel , Bayram Hasan , Oros Georgia Irina
Published: July 2024

This paper establishes new results related to geometric function theory by presenting a new subclass of harmonic functions with complex values within the open unit disk, characterized by a second-order differential inequality. The investigation explores the bounds on the coefficients and estimates of the function growth. This paper also demonstrates that this subclass remains stable under the convolution operation applied to its members. In addition, in the last section, images of the unit disk under some functions of this class are given.
A System of Four Generalized Sylvester Matrix Equations over the Quaternion Algebra
Authors: He Zhuo-Heng , Tian Jie , Yu Shao-Wen
Published: July 2024

In this paper, we make use of the simultaneous decomposition of eight quaternion matrices to study the solvability conditions and general solutions to a system of two-sided coupled Sylvester-type quaternion matrix equations AiXiCi+BiXi+1Di=Ωi,i=1,2,3,4. We design an algorithm to compute the general solution to the system and give a numerical example. Additionally, we consider the application of the system in the encryption and decryption of color images.
Modulation of Apoptotic, Cell Cycle, DNA Repair, and Senescence Pathways by Marine Algae Peptides in Cancer Therapy
Authors: Visuddho Visuddho , Halim Princella , Helen Helen , Muhar Adi Muradi , Iqhrammullah Muhammad , Mayulu Nelly , Surya Reggie , Tjandrawinata Raymond Rubianto , Ribeiro Rosy Iara Maciel Azambuja , Tallei Trina Ekawati , Taslim Nurpudji Astuti , Kim Bonglee , Syahputra Rony Abdi , Nurkolis Fahrul
Published: July 2024

Marine algae, encompassing both macroalgae and microalgae, have emerged as a promising and prolific source of bioactive compounds with potent anticancer properties. Despite their significant therapeutic potential, the clinical application of these peptides is hindered by challenges such as poor bioavailability and susceptibility to enzymatic degradation. To overcome these limitations, innovative delivery systems, particularly nanocarriers, have been explored. Nanocarriers, including liposomes, nanoparticles, and micelles, have demonstrated remarkable efficacy in enhancing the stability, solubility, and bioavailability of marine algal peptides, ensuring controlled release and prolonged therapeutic effects. Marine algal peptides encapsulated in nanocarriers significantly enhance bioavailability, ensuring more efficient absorption and utilization in the body. Preclinical studies have shown promising results, indicating that nanocarrier-based delivery systems can significantly improve the pharmacokinetic profiles and therapeutic outcomes of marine algal peptides. This review delves into the diverse anticancer mechanisms of marine algal peptides, which include inducing apoptosis, disrupting cell cycle progression, and inhibiting angiogenesis. Further research focused on optimizing nanocarrier formulations, conducting comprehensive clinical trials, and continued exploration of marine algal peptides holds great promise for developing innovative, effective, and sustainable cancer therapies.
Regular Physical Activity in the Prevention of Post-Transplant Diabetes Mellitus in Patients after Kidney Transplantation
Authors: Graňák Karol , Vnučák Matej , Beliančinová Monika , Kleinová Patrícia , Blichová Tímea , Pytliaková Margaréta , Dedinská Ivana
Published: July 2024

Background and Objectives: Post-transplant diabetes mellitus (PTDM) is a significant risk factor for the survival of graft recipients and occurs in 10–30% of patients after kidney transplant (KT). PTDM is associated with premature cardiovascular morbidity and mortality. Weight gain, obesity, and dyslipidemia are strong predictors of PTDM, and by modifying them with an active lifestyle it is possible to reduce the incidence of PTDM and affect the long-term survival of patients and grafts. The aim of our study was to determine the effect of regular physical activity on the development of PTDM and its risk factors in patients after KT. Materials and Methods: Participants in the study had to achieve at least 150 min of moderate-intensity physical exertion per week. The study group (n = 22) performed aerobic or combined (aerobic + strength) types of sports activities. Monitoring was provided by the sports tracker (Xiaomi Mi Band 4 compatible with the Mi Fit mobile application). The control group consisted of 22 stable patients after KT. Each patient underwent an oral glucose tolerance test (oGTT) at the end of the follow-up. The patients in both groups have the same immunosuppressive protocol. The total duration of the study was 6 months. Results: The patients in the study group had significantly more normal oGTT results at 6 months compared to the control group (p < 0.0001). In the control group, there were significantly more patients diagnosed with PTDM (p = 0.0212) and with pre-diabetic conditions (impaired plasma glucose and impaired glucose tolerance) at 6 months (p = 0.0078). Conclusions: Regular physical activity after KT provides significant prevention against the development of pre-diabetic conditions and PTDM.
Inside the Hospitalization Voyage of Schizophrenia Care: A Single-Center Journey
Authors: Pop Răzvan , Crișan Cătălina Angela , Micluția Ioana Valentina , Pop Emilia , Iancu Mihaela , Bolboacă Sorana D.
Published: July 2024

Schizophrenia poses significant challenges for individuals and caregivers, often leading to recurrent hospitalizations. Limited information on patients with schizophrenia and multiple hospitalizations in Romania is available in the scientific literature. Our study aimed to evaluate the characteristics of patients with schizophrenia with multiple hospitalizations in a single center in Cluj-Napoca, analyzing if specific patterns exist between patients with two or more hospitalizations or between men and women. We conducted a retrospective study on patients diagnosed with schizophrenia according to the 10th revision of the International Classification of Diseases (ICD 10), hospitalized at the County Emergency Hospital of Cluj-Napoca, Romania, between 2018 and 2022. Data on demographics, somatic comorbidities, symptom severity using the positive and negative syndrome scale (PANSS) or the brief psychiatric rating scale (BPRS), antipsychotic medication, and adverse effects were collected. We evaluated 62 patients, aged from 23 to 57 years, with 157 hospitalizations (ranging from two to seven per patient). No familial history of schizophrenia (56.5%) or bipolar disorder (71%) was reported by most patients. Forty-eight patients were male, and 45 had two hospitalizations. Age, sex, living place and conditions, season of birth, and marital status were similar in patients with two or more than two hospitalizations (p-values > 0.10). Significant differences were observed between patients with two or more than two hospitalizations regarding smoking (63.3% vs. 79.1%, p-value = 0.0029) and symptoms of fear at admission (40.0% vs. 65.7%, p-value = 0.0015). We observed lower scores in the overall PANSS and BPRS scores at discharge compared to admission (p-values < 0.001), regardless of the group (two or more than two hospitalizations, men vs. women). Men and women showed differences in hospitalization stays (median 17.25 vs. 15 days, p-value < 0.001) and BPRS scores at admission (p-value = 0.012) and discharge (p-value = 0.016). Fewer First-Generation Antipsychotics were prescribed for those with two admissions, and nearly half reported adverse effects, notably tachycardia (29%), with similar occurrence within groups. Our results showed that the candidate for multiple hospitalizations is a male, with a mean age of 37 years, unmarried, and living with someone in urban settings, more likely a smoker who exhibits fear symptoms.
The Optimization of the Synthesis of Antibacterial Coatings on Ti6Al4V Coupons Obtained by Electron Beam Melting
Authors: Molina Javier , Valero-Gómez Ana , Bernabé-Quispe Patricia , Tormo-Mas María Ángeles , Bosch Francisco
Published: July 2024

Prosthetic joint infection represents a problem that worsens the patient’s quality of life and produces an economic impact on health systems. We report the anodization of Ti6Al4V coupons obtained by electron beam melting to produce a nanostructured surface. Anodization at 10 V produced TiO2 nanopores with a diameter in the range of 15–20 nm. Thereafter, Ag nanoparticles (AgNPs) were deposited in three different ways to provide antibacterial functionality to the coatings: electrochemically, thermally, and chemically. The electrochemical method did not provide good coverage of AgNPs. At 0.1 V of synthesis potential, cubic, octahedral, and truncated octahedral Ag crystals were obtained. The thermal method provided a good distribution of AgNPs but it damaged the TiO2 nanostructure. The chemical method showed the best distribution of AgNPs over the anodized surface and preserved the anodized nanostructure. For this reason, the chemical method was selected to perform further studies. Ag+ release was monitored in simulated body fluid at 37 °C, reaching 1.86 mg Ag+/L after 42 days. The antibacterial coating showed excellent antibacterial activity and inhibited biofilm formation for Staphylococcus epidermidis RP62A and Staphylococcus aureus V329 strains (lethality > 99.9% for both bacteria and assays).
Epiphytic Patterns Impacting Metabolite Diversity of Drynaria roosii Rhizomes Based on Widely Targeted Metabolomics
Authors: Chang Nana , Yang Xianping , Wang Xiaoqing , Chen Chao , Wang Chu , Xu Yang , Huang Hengyu , Wang Ye
Published: July 2024

Drynaria roosii Nakaike, a fern widely distributed in China and some countries in Southeast Asia, is a commonly used herbal medicine in tonic diets and Chinese patented medicine. The metabolites of its dried rhizomes are easily affected by the epiphytic pattern, whether on rock tunnels (RTs) or tree trunks (TTs). The current research focused on rhizomes from these two patterns, RTs and TTs (further divided into subclasses TA, TB, TC, and TD, based on trunk differences) and conducted a widely targeted metabolomics analysis. A total of 1435 components were identified across 13 categories, with flavonoids, amino acids, and their derivative, lipids, identified as the main components. They accounted for 19.96%, 12.07%, and 12.14% of all metabolites, respectively. The top five flavonoids in TB were eriodicty-ol-7-O-(6″-acetyl)glucoside, quercetin-3-O-sophoroside (baimaside), dihydrochar-cone-4′-O-glucoside, morin, and hesperetin-7-O-glucoside, with relative contents 76.10, 24.20, 17.02, 15.84, and 14.64 times higher than in RTs. Principal component analysis revealed that samples with different epiphytic patterns clustered into five groups. The RT patterns revealed unique metabolites that were not detected in the other four epiphytic species (TA, TB, TC, and TD), including 16 authenticated metabolites: 1 alkaloid, 1 amino acid derivative, 7 flavonoids, 2 lignans, 1 lipid, 1 alcohol, 1 aldehyde, and 2 phenolic acids. These differences in epiphytic patterns considerably affected the accumulation of both primary and secondary metabolites. The comparison of diversity between RTs and TTs can guide the selection of a cultivation substance and the grading of collective rhizomes in the wild. This comprehensive analysis of D. roosii rhizome metabolites also offers fundamental insights for identifying active components and understanding the mechanisms underlying their potential pharmacological activities.
Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide Studies
Authors: Oliva Gianmaria , Vigliotta Giovanni , Di Stasio Luca , Vasca Ermanno , Castiglione Stefano
Published: July 2024

Rare Earth Elements (REE), also known as Lanthanides (Ln3+), are a group of 17 elements showing peculiar physical and chemical properties. Unlike technological applications, very little is known about the physiological role and toxicity of Ln3+ on biological systems, in particular on microorganisms (e.g., bacteria), which represent the most abundant domains on our planet. Up to now, very limited studies have been conducted due to Ln3+ precipitation with some anions commonly present in the culture media. Therefore, the development of a minimal medium is essential to allow the study of Ln3+-microbial interactions, limiting considerably the precipitation of insoluble salts. In this regard, a new minimal culture medium capable of solubilizing large amounts of Ln3+ and allowing the growth of different microbial taxa was successfully developed. Assays have shown that the medium is capable of solubilizing Ln3+ up to 100 times more than other common culture media and allowing the growth of 63 bacteria and 5 fungi. The kinetic growth of one yeast and one Gram-positive bacterium has been defined, proving to support superior growth and biomass compared to other commonly used minimal media. Moreover, the sensitivity and uptake/absorption of a Bacillus stratosphericus strain were tested, highlighting its capability to tolerate concentrations up to 10 mM of either Cerium, Gadolinium or Lanthanum and accumulate different quantities of the three.
Effects of Rehydration on Bacterial Diversity in the Rhizosphere of Broomcorn Millet (Panicum miliaceum L.) after Drought Stress at the Flowering Stage
Authors: Liu Yuhan , Mao Jiao , Xu Yuanmeng , Ren Jiangling , Wang Mengyao , Wang Shu , Liu Sichen , Wang Ruiyun , Wang Lun , Wang Liwei , Qiao Zhijun , Cao Xiaoning
Published: July 2024

This study aimed to elucidate responses of the bacterial structure and diversity of the rhizosphere in flowering broomcorn millet after rehydration following drought stress. In this study, the broomcorn millet varieties ‘Hequ red millet’ (A1) and ‘Yanshu No.10′ (A2), known for their different drought tolerance levels, were selected as experimental materials. The plants were subjected to rehydration after drought stress at the flowering stage, while normal watering (A1CK and A2CK) served as the control. Soil samples were collected at 10 days (A11, A21, A1CK1, and A2CK1) and 20 days (A12, A22, A1CK2, and A2CK2) after rehydration. High-throughput sequencing technology was employed to investigate the variations in bacterial community structure, diversity, and metabolic functions in the rhizosphere of the broomcorn millet at different time points following rehydration. The findings indicated that the operational taxonomic units (OTUs) of bacteria in the rhizosphere of broomcorn millet were notably influenced by the duration of treatment, with a significant decrease in OTUs observed after 20 days of rehydration. However, bacterial Alpha diversity was not significantly impacted by rehydration following drought stress. The bacterial community in the rhizosphere of broomcorn millet was mainly composed of Actinobacteria and Proteobacteria. After rewatering for 10 to 20 days after drought stress, the abundance of Sphingomonas and Aeromicrobium in the rhizosphere soil of the two varieties of broomcorn millet decreased gradually. Compared with Yanshu No.10, the abundance of Pseudarthrobacter in the rhizosphere of Hequ red millet gradually increased. A Beta diversity analysis revealed variations in the dissimilarities of the bacterial community which corresponded to different rehydration durations. The relative abundance of bacterial metabolic functions in the rhizosphere of broomcorn millet was lower after 20 days of rehydration, compared to measurements after 10 days of rehydration. This observation might be attributed to the exchange of materials between broomcorn millet and microorganisms during the initial rehydration stage to repair the effects of drought, as well as to the enrichment of numerous microorganisms to sustain the stability of the community structure. This study helps to comprehend the alterations to the bacterial structure and diversity in the rhizosphere of broomcorn millet following drought stress and rehydration. It sheds light on the growth status of broomcorn millet and its rhizosphere microorganisms under real environmental influences, thereby enhancing research on the drought tolerance mechanisms of broomcorn millet.
Model Sharing and Scalability in the Real-Time Simulation and Intelligent Hierarchical Control of Discrete-Event Systems
Authors: Gonzalez Fernando
Published: July 2024

Large-scale automated systems such as manufacturing systems, transportation systems, the Smart Grid and many others are continuously becoming larger, more distributed, more complex, and more intelligent. There is a growing expectation that their software controller will make real-time intelligent decisions, at all levels of the control hierarchy that make up the enterprise. The need is changing for distributed intelligent controllers that are scalable to arbitrarily large systems. In this paper, we first present the model explosion problem. This problem arises when every controller in the control hierarchy is to have a unique simulation model of its unique control domain to use in its decision-making process. That is, the modeling effort needed to provide intelligence to all controllers in the control hierarchy grows exponentially with the number of controllers in the hierarchy using current modeling technology. Since each controller is in a unique location within the control hierarchy, each will need to have its simulation model custom made for its unique control domain, leading to the scalability issue that we refer to as the model explosion problem. Next, a new modeling paradigm that solves the scalability issue resulting from the model explosion problem is presented, where the simulation models are automatically generated by recycling the models used for control. If the controller models are created using the presented modeling paradigm, then these same models can be used for simulation with no modification or the need to understand the control logic. Furthermore, gathering the state from the physical system being controlled to initialize the simulation models in a real-time control application becomes a trivial operation of simply coping data from one software model to its identical copy, without the need to interpret the meaning of the data. Finally, an example of a hierarchical controller to control a small physical model of a manufacturing plant is presented. We show how we automatically generated all the simulation models in the control hierarchy without any modification and with minimal effort, and used them to make intelligent decisions in real time.
Comparative Analysis of Hydrosol Volatile Components of Citrus × Aurantium ‘Daidai’ and Citrus × Aurantium L. Dried Buds with Different Extraction Processes Using Headspace-Solid-Phase Microextraction with Gas Chromatography–Mass Spectrometry
Authors: Xie Xinyue , Xue Huiling , Ma Baoshan , Guo Xiaoqian , Xia Yanli , Yang Yuxia , Xu Ke , Li Ting , Luo Xia
Published: July 2024

This work used headspace solid-phase microextraction with gas chromatography–mass spectrometry (HS-SPME-GC–MS) to analyze the volatile components of hydrosols of Citrus × aurantium ‘Daidai’ and Citrus × aurantium L. dried buds (CAVAs and CADBs) by immersion and ultrasound–microwave synergistic-assisted steam distillation. The results show that a total of 106 volatiles were detected in hydrosols, mainly alcohols, alkenes, and esters, and the high content components of hydrosols were linalool, α-terpineol, and trans-geraniol. In terms of variety, the total and unique components of CAVA hydrosols were much higher than those of CADB hydrosols; the relative contents of 13 components of CAVA hydrosols were greater than those of CADB hydrosols, with geranyl acetate up to 15-fold; all hydrosols had a citrus, floral, and woody aroma. From the pretreatment, more volatile components were retained in the immersion; the relative contents of linalool and α-terpineol were increased by the ultrasound–microwave procedure; and the ultrasound–microwave procedure was favorable for the stimulation of the aroma of CAVA hydrosols, but it diminished the aroma of the CADB hydrosols. This study provides theoretical support for in-depth exploration based on the medicine food homology properties of CAVA and for improving the utilization rate of waste resources.