bluuebunny/crossref_metadata_2025 huggingface dataset:
ASSESSMENT OF RESIDUAL GEOGENIC CARBON IN MORTARS CONCERNING RADIOCARBON DATING
Authors: Válek Jan , Kozlovcev Petr , Fialová Anna , Kotková Kristýna , Frankeová Dita , Světlík Ivo , Pachnerová Brabcová Kateřina
Published: December 2023

Quicklime samples were collected from six vertical layers (L1–L6) of a feedstock calcined in a traditional single-batch wood-fired kiln and assessed. Three samples were well-burned and three under-burned. The quicklime was slaked in an excess of water and the presence of unburned particles was investigated after settling it into putty. The putty was assessed as bulk and also at three depth levels. Thermal analysis determined the CO2 residua in the quicklime samples. Cathodoluminescence detected individual unburned particles and image analysis was used for their quantification. Settling of the putties led to a considerable reduction of geogenic particles in the layers above the bottom. This was also confirmed by the stable isotope analysis. In the case of the putties made from well-burned quicklime, the δ13C values of samples L4 and L5 ranged from –25.5‰ to –20.5‰ VPDB, and the δ18O values ranged from –17.5‰ to –16.5‰ VPDB. The fractionation was likely affected by the division according to the particle size during the sedimentation. The results of the 14C analysis correlate with the quantified percentage of cathodoluminescent particles.
From Social Networks to Political Parties: Indigenous Party-Building in Bolivia
Authors: GIUSTI-RODRÍGUEZ MARIANA
Published: December 2023

While existing scholarship recognizes the centrality of social organizations for party-building efforts, how network structures condition party-building remains underexamined. This article argues that a core property of the network environments within which proto-parties emerge—structural resilience—shapes opportunities for proto-parties’ expansion and consolidation. More resilient network structures—those with multiple pathways available for expansion—decrease proto-parties’ vulnerability to structural threats and allow them to circumvent competition. To evaluate this theory, I examine the organizational networks of three comparable indigenous party-building efforts in Bolivia. Using original network data and a mixed-methods approach, I demonstrate that MAS-IPSP succeeded in establishing itself as the indigenous party because of the structural resilience of the network environment within which it originated. By contrast, its counterparts failed when targeted network attacks undermined their access to organizational spaces critical to their expansion strategies. The findings reveal often-overlooked variation in the relationship between social organizations and political parties.
The effect of a low-calorie diet on depressive symptoms in individuals with overweight or obesity: a systematic review and meta-analysis of interventional studies
Authors: Applewhite Briana , Penninx Brenda W. J. H. , Young Allan H. , Schmidt Ulrike , Himmerich Hubertus , Keeler Johanna L.
Published: December 2023

Background

Individuals with overweight or obesity are at a high risk for so-called ‘atypical’ or immunometabolic depression, with associated neurovegetative symptoms including overeating, fatigue, weight gain, and a poor metabolic profile evidenced e.g. by dyslipidemia or hyperglycemia. Research has generated preliminary evidence for a low-calorie diet (LCD) in reducing depressive symptoms. The aim of the current systematic review and meta-analysis is to examine this evidence to determine whether a LCD reduces depressive symptoms in people with overweight or obesity.

Methods

Eligible studies were identified through PubMed, ISI Web of Science, and PsycINFO until August 2023. Standardized mean differences (SMDs) were derived using random-effects meta-analyses for (1) pre-post LCD comparisons of depression outcomes, and (2) LCD v. no-diet-control group comparisons of depression outcomes.

Results

A total of 25 studies were included in the pre-post meta-analysis, finding that depression scores were significantly lower following a LCD (SMD = −0.47), which was not significantly moderated by the addition of exercise or behavioral therapy as a non-diet adjunct. Meta-regressions indicated that a higher baseline BMI and greater weight reduction were associated with a greater reduction in depression scores. The intervention-control meta-analysis (n = 4) found that overweight or obese participants adhering to a LCD showed a nominally lower depression score compared with those given no intervention (SMD = −0.29).

Conclusions

There is evidence that LCDs may reduce depressive symptoms in people with overweight or obesity in the short term. Future well-controlled intervention studies, including a non-active control group, and longer-term follow-ups, are warranted in order to make more definitive conclusions.
Robust evidence reveals the reliable rate of normal/balanced embryos for identifying reciprocal translocation and Robertsonian translocation carriers
Authors: Tian Zhihua , Lian Wenchang , Xu Li , Long Yanxi , Tang Li , Wang Huawei
Published: December 2023

We aimed to evaluate the reliable rate of normal/balanced embryos for reciprocal translocation and Robertsonian translocation carriers and to provide convincing evidence for clinical staff to conduct genetic counselling regarding common structural rearrangements to alleviate patient anxiety. The characteristics of 39,459 embryos that were sourced from unpublished data and literature were analyzed. The samples consisted of 17,536 embryo karyotypes that were not published and 21,923 embryo karyotypes obtained from the literature. Using the PubMed, Cochrane Library, Web of Science, and Embase databases, specific keywords were used to screen the literature for reciprocal translocation and Robertsonian translocation. The ratio of normal/balanced embryos in the overall data was calculated and analyzed, and we grouped the results according to gender to confirm if there were gender differences. We also divided the data into the cleavage stage and blastocyst stage according to the biopsy period to verify if there was a difference in the ratio of normal/balanced embryos. By combining the unpublished data and data derived from the literature, the average rates of normal/balanced embryos for reciprocal translocation and Robertsonian translocation carriers were observed to be 26.96% (7953/29,495) and 41.59% (4144/9964), respectively. Reciprocal translocation and Robertson translocation exhibited higher rates in male carriers than they did in female carriers (49.60% vs. 37.44%; 29.84% vs. 27.67%). Additionally, the data for both translocations exhibited differences in the normal/balanced embryo ratios between the cleavage and blastocyst stages of carriers for both Robertsonian translocation and reciprocal translocation (36.07% vs 43.43%; 24.88% vs 27.67%). The differences between the two location types were statistically significant (P < 0.05). The normal/balanced ratio of embryos in carriers of reciprocal and RobT was higher than the theoretical ratio, and the values ranged from 26.96% to 41.59%. Moreover, the male carriers possessed a higher number of embryos that were normal or balanced. The ratio of normal/balanced embryos in the blastocyst stage was higher than that in the cleavage stage. The results of this study provide a reliable suggestion for future clinic genetic consulting regarding the rate of normal/balanced embryos of reciprocal translocation and Robertsonian translocation carriers.
Trans-catheter closure of an unusual type of Abernethy malformation in a child presenting with severe pulmonary hypertension
Authors: Wadile Santosh , Banpurkar Ashishkumar , Kulkarni Snehal
Published: December 2023

Abernethy malformation is a congenital extra-hepatic porto-systemic shunt. This malformation is characterized by an abnormal connection between the portal vein or its branches and one of the systemic veins. Though rare, this anomaly can lead to pulmonary hypertension. Drainage of Abernethy malformation into coronary sinus is extremely rare. We describe a child with Abernethy malformation with unusual drainage into coronary sinus. The abnormal channel was successfully closed by trans-catheter technique with normalisation of pulmonary arterial pressures.
Two decades of Social Security claiming
Authors: Slavov Sita Nataraj
Published: December 2023

Twenty years ago, the adjustment to monthly Social Security benefits for early or delayed claiming was, on average, roughly actuarially fair, although some subsets of individuals could gain from delay. Since then, delaying claiming has become much more attractive thanks to three factors: a more generous delayed retirement credit, improvements in mortality, and historically low real interest rates. In this article, I examine how these three factors influence optimal claiming behavior. I also discuss empirical patterns of claiming across individuals and over time, as well as explanations for these patterns. I argue that although many people appear to claim suboptimally early, this behavior may be changing as information spreads about the importance of the claiming decision. Finally, I discuss policy toward claiming and the impact that an increase in strategic claiming could have on Social Security's finances.
Justice in benefitting from carbon removal
Authors: Lenzi Dominic , Schübel Hanna , Wallimann-Helmer Ivo
Published: December 2023

Non-Technical Summary

Climate stabilization requires scaling-up technologies to capture and store carbon. Carbon removal could be very profitable, and some of the agents best placed to benefit are ‘carbon majors’, i.e. fossil fuel companies. We argue that in ordinary circumstances only agents without significant historical climate responsibilities would be entitled to the full benefits from carbon removal. Under non-ideal conditions, carbon majors might be entitled to benefit, provided that no other agent could remove similar quantities of carbon at similar costs. This burden of proof is only likely to be met in countries with poor governance capacities.

Technical Summary

Climate stabilization requires scaling up technologies to capture and store carbon. Some of the agents best placed to profit from carbon removal are ‘carbon majors’, especially fossil fuel companies. Yet incentivizing carbon majors to undertake carbon removal poses an ethical dilemma: carbon majors have made significant historical contributions to climate change and have significantly benefitted from such contributions without being made to compensate for resulting climate harm. This is why it seems unfair to reward them with additional economic benefits. However, carbon majors possess the technological skills and infrastructure to upscale carbon removal efficiently. We argue that in ordinary circumstances, only agents without significant climate responsibilities would be morally entitled to fully benefit from carbon removal. Yet under non-ideal conditions, it might be permissible to reward carbon majors if no other agent were capable of removing as much carbon at similar costs and on similar timeframes. We believe this argument faces an imposing burden of proof that is only likely to be met in countries with poor governance capacities. In more favorable circumstances, including those of most OECD countries, rewarding carbon majors without having them pay for their historical climate responsibilities remains impermissible.

Social Media Summary

Rewarding carbon majors to undertake carbon dioxide removal is unjust due to their historical climate responsibilities. Where possible, governments should empower other agents to remove CO2.
Observing Temporally Varying Synoptic‐Scale Total Alkalinity and Dissolved Inorganic Carbon in the Arctic Ocean
Authors: Green Hannah L. , Findlay Helen S. , Shutler Jamie D. , Sims Richard , Bellerby Richard , Land Peter E.
Published: December 2023

The long‐term absorption by the oceans of atmospheric carbon dioxide is leading to the slow decline of ocean pH, a process termed ocean acidification (OA). The Arctic is a challenging region to gather enough data to examine the changes in carbonate chemistry over sufficient scales. However, algorithms that calculate carbonate chemistry parameters from more frequently measured parameters, such as temperature and salinity, can be used to fill in data gaps. Here, these published algorithms were evaluated against in situ measurements using different data input types (data from satellites or in situ re‐analysis climatologies) across the Arctic Ocean. With the lowest uncertainties in the Atlantic influenced Seas (AiS), where re‐analysis inputs achieved total alkalinity estimates with Root Mean Squared Deviation (RMSD) of 21 μmol kg−1 and a bias of 2 μmol kg−1 (n = 162) and dissolved inorganic carbon RMSD of 24 μmol kg−1 and bias of −14 μmol kg−1 (n = 262). AiS results using satellite observation inputs show similar bias but larger RMSD, although due to the shorter time span of available satellite observations, more contemporary in situ data would provide further assessment and improvement. Synoptic‐scale observations of surface water carbonate conditions in the Arctic are now possible to monitor OA, but targeted in situ data collection is needed to enable the full exploitation of satellite observation‐based approaches.
Automated Detection and Characterization of Wave Structures Obtained From GNSS Measurements
Authors: Jonah Olusegun , Reyes Pablo , Lamarche Leslie , van Eyken Anthony
Published: December 2023

A simple technique designed to automatically identify and characterize wave structures from total electron content (TEC) data obtained from Global Navigation Satellite System (GNSS) satellites and multiple receiver stations is presented. We used 11 years of GNSS data (one complete solar cycle) to detect and characterize the traveling ionospheric disturbances (TIDs) (or wave structures) over high latitude (65°N ± 5°, 147°W ± 5°—Poker Flat, AK) and middle latitude (40°N ± 5°, 117°W ± 5°—Mt. Moses, NV) regions. The algorithm is capable of automatically detecting basic wave parameters such as wave period, horizontal phase velocity, amplitude, wave propagation direction, and wavelength. The designed algorithm can be applicable in the following areas: (a) global GNSS‐TEC (b) climatology of waves (c) input into innovative machine learning algorithms, such as ABCGAN (e.g., Valentic, 2023, https://doi.org/10.5281/zenodo.7747377 designed to characterize background ionospheric plasma and predict wave‐like high/low‐frequency perturbations) (d) anomaly detection for real‐time scenarios. Furthermore, we apply the developed wave detect and characterization technique to investigate three rockets launched on 26 and 28 January 2015. We examine the distribution of different scales of TIDs, and how they varied from high (Poker Flat) to middle (Mt. Moses) latitudes. Lastly, we show that the wave structures at the high‐latitude regions of Poker Flat are substantially affected by auroral processes and those from the middle‐latitude regions of Mt. Moses are impacted by AGWs coupling from below.
The Crustal Magmatic Structure Beneath the Denali Volcanic Gap Imaged by a Dense Linear Seismic Array
Authors: Rabade Santiago , Lin Fan‐Chi , Tape Carl , Ward Kevin M. , Waldien Trevor , Allam Amir
Published: December 2023

The crustal structure in south‐central Alaska has been influenced by terrane accretion, flat slab subduction, and a modern strike‐slip fault system. Within the active subduction system, the presence of the Denali Volcanic Gap (DVG), a ∼400 km region separating the active volcanism of the Aleutian Arc to the west and the Wrangell volcanoes to the east, remains enigmatic. To better understand the regional tectonics and the nature of the volcanic gap, we deployed a month‐long north‐south linear geophone array of 306 stations with an interstation distance of 1 km across the Alaska Range. By calculating multi‐component noise cross‐correlation and jointly inverting Rayleigh wave phase velocity and ellipticity across the array, we construct a 2‐D shear wave velocity model along the transect down to ∼16 km depth. In the shallow crust, we observe low‐velocity structures associated with sedimentary basins and image the Denali fault as a narrow localized low‐velocity anomaly extending to at least 12 km depth. About 12 km, below the fold and thrust fault system in the northern flank of the Alaska Range, we observe a prominent low‐velocity zone with more than 15% velocity reduction. Our velocity model is consistent with known geological features and reveals a previously unknown low‐velocity zone that we interpret as a magmatic feature. Based on this feature's spatial relationship to the Buzzard Creek and Jumbo Dome volcanoes and the location above the subducting Pacific Plate, we interpret the low‐velocity zone as a previously unknown subduction‐related crustal magma reservoir located beneath the DVG.
Synthetic Aperture Radar Imaging Below a Random Rough Surface
Authors: Kim Arnold D. , Tsogka Chrysoula
Published: December 2023

Motivated by applications in unmanned aerial based ground penetrating radar for detecting buried landmines, we consider the problem of imaging small point like scatterers situated in a lossy medium below a random rough surface. Both the random rough surface and the absorption in the lossy medium significantly impede the target detection and imaging process. Using principal component analysis we effectively remove the reflection from the air‐soil interface. We then use a modification of the classical synthetic aperture radar imaging functional to image the targets. This imaging method introduces a user‐defined parameter, δ, which scales the resolution by allowing for target localization with sub wavelength accuracy. Numerical results in two dimensions illustrate the robustness of the approach for imaging multiple targets. However, the depth at which targets are detectable is limited due to the absorption in the lossy medium.
Shale 3D Flow and Interaction With Basement Faults in the Niger Delta Deep‐Water Fold and Thrust Belt
Authors: Spina V. , Mazzoli S.
Published: December 2023

Based on a large 3D seismic data set in the deep‐water domain of the Niger Delta, this study challenges previous interpretations involving the occurrence of multiple detachments and extensive thrust flats, illustrating timing and mode of shales flow at the toe of the gravity system. Five units of syn‐kinematic sediments, reaching a maximum thickness of ∼800 m, accumulated in the tectonically subsiding synclines during fold amplification between ∼9.5 and ∼1.4 Ma. The volumes of syn‐kinematic units roughly balance those of the shales accumulated in the thickened cores of WNW trending anticlines. This feature is consistent with folding resulting from buckling controlled by the competence contrast between isopach Cenozoic units and underlying overpressured shales of the Akata Formation. A dense network of NE‐SW striking oblique extensional faults offsets a prominent anticline characterized by a NE‐SW trend (which is almost perpendicular to the regional fold trend). These faults form a narrow, continuous deformation zone extending for tens of kilometers along and beyond the length of the anticline. The faults, rooting within the shales of the Akata Formation, formed since ∼5 Ma and deform the seabed. Displacement distribution suggests mechanical interaction between isolated fault segments within the deformation zone. The latter is interpreted as the shallow expression of a deep‐seated fault zone inherited from the segmented passive margin and marked by gravity and magnetic data. Our results, providing a comprehensive picture of active deformation features and their relationships with deep‐seated faults, shed new light into the modes of interaction between gravity systems and underlying basement structures.
Panel-based RNA fusion sequencing improves diagnostics of pediatric acute myeloid leukemia
Authors: Hoffmeister Lina Marie , Suttorp Julia , Walter Christiane , Antoniou Evangelia , Behrens Yvonne Lisa , Göhring Gudrun , Awada Amani , von Neuhoff Nils , Reinhardt Dirk , Schneider Markus
Published: December 2023

New methods like panel-based RNA fusion sequencing (RNA-FS) promise improved diagnostics in various malignancies. We here analyzed the impact of RNA-FS on the initial diagnostics of 241 cases with pediatric acute myeloid leukemia (AML). We show that, compared to classical cytogenetics (CCG), RNA-FS reliably detected risk-relevant fusion genes in pediatric AML. In addition, RNA-FS strongly improved the detection of cryptic fusion genes like NUP98::NSD1, KMT2A::MLLT10 and CBFA2T3::GLIS2 and thereby resulted in an improved risk stratification in 25 patients (10.4%). Validation of additionally detected non-risk-relevant high confidence fusion calls identified PIM3::BRD1, C22orf34::BRD1, PSPC1::ZMYM2 and ARHGAP26::NR3C1 as common genetic variants and MYB::GATA1 as recurrent aberration, which we here describe in AML subtypes M0 and M7 for the first time. However, it failed to detect rare cytogenetically confirmed fusion events like MNX1::ETV6 and other chromosome 12p-abnormalities. As add-on benefit, the proportion of patients for whom measurable residual disease (MRD) monitoring became possible was increased by RNA-FS from 44.4 to 75.5% as the information on the fusion transcripts’ sequence allowed the design of new MRD assays.
Activation of GPER1 in macrophages ameliorates UUO-induced renal fibrosis
Authors: Xie Lin , Cheng Ye , Du Wen , Fu Lili , Wei Zhaonan , Guan Yuting , Wang Yi , Mei Changlin , Hao Chuanming , Chen Min , Gu Xiangchen
Published: December 2023

Numerous studies have proven the critical role of macrophages in the renal fibrosis process. Notably, G Protein-coupled Estrogen Receptor 1 (GPER1), a novel estrogen receptor, has been shown to play a ubiquitous role in regulating macrophage activities and proinflammatory pathways. However, the precise role of GPER1 in macrophage-mediated renal fibrosis is unknown. In this study, we aimed to investigate the function of macrophage GPER1 in the UUO-induced renal fibrosis model. Compared to vehicle-treated ovariectomized (OVX) female and male unilateral ureteral obstruction (UUO) models, we observed that G-1 (GPER1 agonist)-treated OVX female and male UUO mice had fewer renal fibrotic lesions and less M1 and M2 macrophage infiltration in the kidney tissues. Conversely, Gper1 deletion in male UUO mice accelerated renal fibrosis and increased inflammation. In vitro studies also revealed that GPER1 activation reduced M0 macrophage polarization towards M1 or M2 phenotypes. The RNA-sequencing analysis and immunoblotting indicated that GPER1 activation was primarily involved in downregulating immune pathways activation and inactivating MAPK pathways. Tubular epithelial cells co-cultured with G-1-pretreated M1 macrophages exhibited fewer injuries and immune activation. In addition, fibroblasts co-cultured with G-1-pretreated M2 macrophages showed downregulated extracellular matrix expression. Overall, this is the first study to demonstrate the effect of GPER1 on macrophage-mediated renal fibrosis via inhibition of M1 and M2 macrophage activation. These findings indicate that GPER1 may be a promising therapeutic target for treating renal fibrosis.
Inhibition of PFKP in renal tubular epithelial cell restrains TGF-β induced glycolysis and renal fibrosis
Authors: Yang Shu , Wu Han , Li Yanchun , Li Lixin , Xiang Jiaqing , Kang Lin , Yang Guangyan , Liang Zhen
Published: December 2023

Metabolic reprogramming to glycolysis is closely associated with the development of chronic kidney disease (CKD). Although it has been reported that phosphofructokinase 1 (PFK) is a rate-limiting enzyme in glycolysis, the role of the platelet isoform of PFK (PFKP) in kidney fibrosis initiation and progression is as yet poorly understood. Here, we investigated whether PFKP could mediate the progression of kidney interstitial fibrosis by regulating glycolysis in proximal tubular epithelial cells (PTECs). We induced PFKP overexpression or knockdown in renal tubules via an adeno-associated virus (AAV) vector in the kidneys of mice following unilateral ureteral occlusion. Our results show that the dilated tubules, the area of interstitial fibrosis, and renal glycolysis were promoted by proximal tubule-specific overexpression of PFKP, and repressed by knockdown of PFKP. Furthermore, knockdown of PFKP expression restrained, while PFKP overexpression promoted TGF-β1-induced glycolysis in the human PTECs line. Mechanistically, Chip-qPCR revealed that TGF-β1 recruited the small mothers against decapentaplegic (SMAD) family member 3-SP1 complex to the PFKP promoter to enhance its expression. Treatment of mice with isorhamnetin notably ameliorated PTEC-elevated glycolysis and kidney fibrosis. Hence, our results suggest that PFKP mediates the progression of kidney interstitial fibrosis by regulating glycolysis in PTECs.
Spectroscopy of a mesospheric ghost reveals iron emissions
Authors: Passas-Varo María , Van der Velde Oscar , Gordillo-Vázquez Francisco J. , Gómez-Martín Juan Carlos , Sánchez Justo , Pérez-Invernón Francisco J. , Sánchez-Ramírez Rubén , García-Comas Maya , Montanyà Joan
Published: December 2023

Mesospheric Green emissions from excited Oxygen in Sprite Tops (ghosts) are infrequent and faint greenish transient luminous events that remain for hundreds of milliseconds on top of certain energetic sprites. The main hypothesis to explain this glow persistence is the long lifetime of excited atomic oxygen at 557.73 nm, a well-known emission line in aurora and airglow. However, due to the lack of spectroscopic campaigns to analyse such events to date, the species involved in the process can not yet be identified. Here we report observational results showing the temporal evolution of a ghost spectrum between 500 nm and 600 nm. Besides weak -but certain- traces of excited atomic oxygen, our results show four main contributors related to the slow decay of the glow: atomic iron and nickel, molecular nitrogen and ionic molecular oxygen. Additionally, we are able to identify traces of atomic sodium, and ionic silicon, these observations being consistent with previous direct measurements of density profiles of meteoric metals in the mesosphere and lower thermosphere. This finding calls for an upgrade of current air plasma kinetic understanding under the influence of transient luminous events.
ZNF524 directly interacts with telomeric DNA and supports telomere integrity
Authors: Braun Hanna , Xu Ziyan , Chang Fiona , Viceconte Nikenza , Rane Grishma , Levin Michal , Lototska Liudmyla , Roth Franziska , Hillairet Alexia , Fradera-Sola Albert , Khanchandani Vartika , Sin Zi Wayne , Yong Wai Khang , Dreesen Oliver , Yang Yang , Shi Yunyu , Li Fudong , Butter Falk , Kappei Dennis
Published: December 2023

Telomeres are nucleoprotein structures at the ends of linear chromosomes. In humans, they consist of TTAGGG repeats, which are bound by dedicated proteins such as the shelterin complex. This complex blocks unwanted DNA damage repair at telomeres, e.g. by suppressing nonhomologous end joining (NHEJ) through its subunit TRF2. Here, we describe ZNF524, a zinc finger protein that directly binds telomeric repeats with nanomolar affinity, and reveal base-specific sequence recognition by cocrystallization with telomeric DNA. ZNF524 localizes to telomeres and specifically maintains the presence of the TRF2/RAP1 subcomplex at telomeres without affecting other shelterin members. Loss of ZNF524 concomitantly results in an increase in DNA damage signaling and recombination events. Overall, ZNF524 is a direct telomere-binding protein involved in the maintenance of telomere integrity.
Daily vocal exercise is necessary for peak performance singing in a songbird
Authors: Adam Iris , Riebel Katharina , Stål Per , Wood Neil , Previs Michael J. , Elemans Coen P. H.
Published: December 2023

Vocal signals, including human speech and birdsong, are produced by complicated, precisely coordinated body movements, whose execution is fitness-determining in resource competition and mate choice. While the acquisition and maintenance of motor skills generally requires practice to develop and maintain both motor circuitry and muscle performance, it is unknown whether vocal muscles, like limb muscles, exhibit exercise-induced plasticity. Here, we show that juvenile and adult zebra finches (Taeniopygia castanotis) require daily vocal exercise to first gain and subsequently maintain peak vocal muscle performance. Experimentally preventing male birds from singing alters both vocal muscle physiology and vocal performance within days. Furthermore, we find females prefer song of vocally exercised males in choice experiments. Vocal output thus contains information on recent exercise status, and acts as an honest indicator of past exercise investment in songbirds, and possibly in all vocalising vertebrates.
A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase
Authors: Oh Juntaek , Shan Zelin , Hoshika Shuichi , Xu Jun , Chong Jenny , Benner Steven A. , Lyumkis Dmitry , Wang Dong
Published: December 2023

Artificially Expanded Genetic Information Systems (AEGIS) add independently replicable unnatural nucleotide pairs to the natural G:C and A:T/U pairs found in native DNA, joining the unnatural pairs through alternative modes of hydrogen bonding. Whether and how AEGIS pairs are recognized and processed by multi-subunit cellular RNA polymerases (RNAPs) remains unknown. Here, we show that E. coli RNAP selectively recognizes unnatural nucleobases in a six-letter expanded genetic system. High-resolution cryo-EM structures of three RNAP elongation complexes containing template-substrate UBPs reveal the shared principles behind the recognition of AEGIS and natural base pairs. In these structures, RNAPs are captured in an active state, poised to perform the chemistry step. At this point, the unnatural base pair adopts a Watson-Crick geometry, and the trigger loop is folded into an active conformation, indicating that the mechanistic principles underlying recognition and incorporation of natural base pairs also apply to AEGIS unnatural base pairs. These data validate the design philosophy of AEGIS unnatural basepairs. Further, we provide structural evidence supporting a long-standing hypothesis that pair mismatch during transcription occurs via tautomerization. Together, our work highlights the importance of Watson-Crick complementarity underlying the design principles of AEGIS base pair recognition.
Second quantization of many-body dispersion interactions for chemical and biological systems
Authors: Gori Matteo , Kurian Philip , Tkatchenko Alexandre
Published: December 2023

The many-body dispersion (MBD) framework is a successful approach for modeling the long-range electronic correlation energy and optical response of systems with thousands of atoms. Inspired by field theory, here we develop a second-quantized MBD formalism (SQ-MBD) that recasts a system of atomic quantum Drude oscillators in a Fock-space representation. SQ-MBD provides: (i) tools for projecting observables (interaction energy, transition multipoles, polarizability tensors) on coarse-grained representations of the atomistic system ranging from single atoms to large structural motifs, (ii) a quantum-information framework to analyze correlations and (non)separability among fragments in a given molecular complex, and (iii) a path toward the applicability of the MBD framework to molecular complexes with even larger number of atoms. The SQ-MBD approach offers conceptual insights into quantum fluctuations in molecular systems and enables direct coupling of collective plasmon-like MBD degrees of freedom with arbitrary environments, providing a tractable computational framework to treat dispersion interactions and polarization response in intricate systems.
Late-stage synthesis of heterobifunctional molecules for PROTAC applications via ruthenium-catalysed C‒H amidation
Authors: Antermite Daniele , Friis Stig D. , Johansson Johan R. , Putra Okky Dwichandra , Ackermann Lutz , Johansson Magnus J.
Published: December 2023

PROteolysis TArgeting Chimeras (PROTACs) are heterobifunctional molecules emerging as a powerful modality in drug discovery, with the potential to address outstanding medical challenges. However, the synthetic feasibility of PROTACs, and the empiric and complex nature of their structure-activity relationships continue to present formidable limitations. As such, modular and reliable approaches to streamline the synthesis of these derivatives are highly desirable. Here, we describe a robust ruthenium-catalysed late-stage C‒H amidation strategy, to access fully elaborated heterobifunctional compounds. Using readily available dioxazolone reagents, a broad range of inherently present functional groups can guide the C–H amidation on complex bioactive molecules. High selectivity and functional group tolerance enable the late-stage installation of linkers bearing orthogonal functional handles for downstream elaboration. Finally, the single-step synthesis of both CRBN and biotin conjugates is demonstrated, showcasing the potential of this methodology to provide efficient and sustainable access to advanced therapeutics and chemical biology tools.
Large scale purification in semiconductors using Rydberg excitons
Authors: Bergen Martin , Walther Valentin , Panda Binodbihari , Harati Mariam , Siegeroth Simon , Heckötter Julian , Aßmann Marc
Published: December 2023

Improving the quantum coherence of solid-state systems is a decisive factor in realizing solid-state quantum technologies. The key to optimize quantum coherence lies in reducing the detrimental influence of noise sources such as spin noise and charge noise. Here we demonstrate that we can utilize highly-excited Rydberg excitons to neutralize charged impurities in the semiconductor Cuprous Oxide - an effect we call purification. Purification reduces detrimental electrical stray fields drastically. We observe that the absorption of the purified crystal increases by up to 25% and that the purification effect is long-lived and may persist for hundreds of microseconds or even longer. We investigate the interaction between Rydberg excitons and impurities and find that it is long-ranged and based on charge-induced dipole interactions. Using a time-resolved pump-probe technique, we can discriminate purification from Rydberg blockade, which has been a long-standing goal in excitonic Rydberg systems.
The second Venus flyby of BepiColombo mission reveals stable atmosphere over decades
Authors: Helbert Jörn , Haus Rainer , Arnold Gabriele , D’Amore Mario , Maturilli Alessandro , Säuberlich Thomas , Hiesinger Harald
Published: December 2023

Studies of the Venusian mesosphere provide important information about the current state of the entire Venusian atmosphere. This includes information about the dense cloud structure, its vertical thermal profile, temperature fields, and the resulting dynamical and meteorological processes that contribute to a deeper understanding of the climatologically different evolutionary paths of Earth and Venus. However, the last measurements were acquired in 1983 during Venera-15 mission. In this paper, results of mid-infrared spectral measurements of the Venusian atmosphere are presented. Here we show Mercury Radiometer and Thermal Infrared Spectrometer (MERTIS) measurements of the Venusian atmosphere during the second flyby of BepiColombo mission on its way to Mercury. Our Venus measurements provide reliable retrievals of mesospheric temperature profiles and cloud parameters between 60 and 75 km altitude, although MERTIS was only designed to operate in Mercury environment. Our results are in good agreement with the Venera-15 mission findings. This indicates the stability of the Venusian atmosphere on time scales of decades.
Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis
Authors: Chaube Balkrishna , Citrin Kathryn M. , Sahraei Mahnaz , Singh Abhishek K. , de Urturi Diego Saenz , Ding Wen , Pierce Richard W. , Raaisa Raaisa , Cardone Rebecca , Kibbey Richard , Fernández-Hernando Carlos , Suárez Yajaira
Published: December 2023

Angiopoietin-like 4 (ANGPTL4) is known to regulate various cellular and systemic functions. However, its cell-specific role in endothelial cells (ECs) function and metabolic homeostasis remains to be elucidated. Here, using endothelial-specific Angptl4 knock-out mice (Angptl4iΔEC), and transcriptomics and metabolic flux analysis, we demonstrate that ANGPTL4 is required for maintaining EC metabolic function vital for vascular permeability and angiogenesis. Knockdown of ANGPTL4 in ECs promotes lipase-mediated lipoprotein lipolysis, which results in increased fatty acid (FA) uptake and oxidation. This is also paralleled by a decrease in proper glucose utilization for angiogenic activation of ECs. Mice with endothelial-specific deletion of Angptl4 showed decreased pathological neovascularization with stable vessel structures characterized by increased pericyte coverage and reduced permeability. Together, our study denotes the role of endothelial-ANGPTL4 in regulating cellular metabolism and angiogenic functions of EC.
D-serine reconstitutes synaptic and intrinsic inhibitory control of pyramidal neurons in a neurodevelopmental mouse model for schizophrenia
Authors: Zhang Xiao-Qin , Xu Le , Zhu Xin-Yi , Tang Zi-Hang , Dong Yi-Bei , Yu Zhi-Peng , Shang Qing , Wang Zheng-Chun , Shen Hao-Wei
Published: December 2023

The hypothesis of N-methyl-D-aspartate receptor (NMDAR) dysfunction for cognitive impairment in schizophrenia constitutes the theoretical basis for the translational application of NMDAR co-agonist D-serine or its analogs. However, the cellular mechanism underlying the therapeutic effect of D-serine remains unclear. In this study, we utilize a mouse neurodevelopmental model for schizophrenia that mimics prenatal pathogenesis and exhibits hypoexcitability of parvalbumin-positive (PV) neurons, as well as PV-preferential NMDAR dysfunction. We find that D-serine restores excitation/inhibition balance by reconstituting both synaptic and intrinsic inhibitory control of cingulate pyramidal neurons through facilitating PV excitability and activating small-conductance Ca2+-activated K+ (SK) channels in pyramidal neurons, respectively. Either amplifying inhibitory drive via directly strengthening PV neuron activity or inhibiting pyramidal excitability via activating SK channels is sufficient to improve cognitive function in this model. These findings unveil a dual mechanism for how D-serine improves cognitive function in this model.
Hot luminescence from single-molecule chromophores electrically and mechanically self-decoupled by tripodal scaffolds
Authors: Rai Vibhuti , Balzer Nico , Derenbach Gabriel , Holzer Christof , Mayor Marcel , Wulfhekel Wulf , Gerhard Lukas , Valášek Michal
Published: December 2023

Control over the electrical contact to an individual molecule is one of the biggest challenges in molecular optoelectronics. The mounting of individual chromophores on extended tripodal scaffolds enables both efficient electrical and mechanical decoupling of individual chromophores from metallic leads. Core-substituted naphthalene diimides fixed perpendicular to a gold substrate by a covalently attached extended tripod display high stability with well-defined and efficient electroluminescence down to the single-molecule level. The molecularly controlled spatial arrangement balances the electric conduction for electroluminescence and the insulation to avoid non-radiative carrier recombination, enabling the spectrally and spatially resolved electroluminescence of individual self-decoupled chromophores in a scanning tunneling microscope. Hot luminescence bands are even visible in single self-decoupled chromophores, documenting the mechanical decoupling between the vibrons of the chromophore and the substrate.
Emergent zero-field anomalous Hall effect in a reconstructed rutile antiferromagnetic metal
Authors: Wang Meng , Tanaka Katsuhiro , Sakai Shiro , Wang Ziqian , Deng Ke , Lyu Yingjie , Li Cong , Tian Di , Shen Shengchun , Ogawa Naoki , Kanazawa Naoya , Yu Pu , Arita Ryotaro , Kagawa Fumitaka
Published: December 2023

The anomalous Hall effect (AHE) that emerges in antiferromagnetic metals shows intriguing physics and offers numerous potential applications. Magnets with a rutile crystal structure have recently received attention as a possible platform for a collinear-antiferromagnetism-induced AHE. RuO2 is a prototypical candidate material, however the AHE is prohibited at zero field by symmetry because of the high-symmetry [001] direction of the Néel vector at the ground state. Here, we show AHE at zero field in Cr-doped rutile, Ru0.8Cr0.2O2. The magnetization, transport and density functional theory calculations indicate that appropriate doping of Cr at Ru sites reconstructs the collinear antiferromagnetism in RuO2, resulting in a rotation of the Néel vector from [001] to [110] while maintaining a collinear antiferromagnetic state. The AHE with vanishing net moment in the Ru0.8Cr0.2O2 exhibits an orientation dependence consistent with the [110]-oriented Hall vector. These results demonstrate that material engineering by doping is a useful approach to manipulate AHE in antiferromagnetic metals.
A deep unrolled neural network for real-time MRI-guided brain intervention
Authors: He Zhao , Zhu Ya-Nan , Chen Yu , Chen Yi , He Yuchen , Sun Yuhao , Wang Tao , Zhang Chengcheng , Sun Bomin , Yan Fuhua , Zhang Xiaoqun , Sun Qing-Fang , Yang Guang-Zhong , Feng Yuan
Published: December 2023

Accurate navigation and targeting are critical for neurological interventions including biopsy and deep brain stimulation. Real-time image guidance further improves surgical planning and MRI is ideally suited for both pre- and intra-operative imaging. However, balancing spatial and temporal resolution is a major challenge for real-time interventional MRI (i-MRI). Here, we proposed a deep unrolled neural network, dubbed as LSFP-Net, for real-time i-MRI reconstruction. By integrating LSFP-Net and a custom-designed, MR-compatible interventional device into a 3 T MRI scanner, a real-time MRI-guided brain intervention system is proposed. The performance of the system was evaluated using phantom and cadaver studies. 2D/3D real-time i-MRI was achieved with temporal resolutions of 80/732.8 ms, latencies of 0.4/3.66 s including data communication, processing and reconstruction time, and in-plane spatial resolution of 1 × 1 mm2. The results demonstrated that the proposed method enables real-time monitoring of the remote-controlled brain intervention, and showed the potential to be readily integrated into diagnostic scanners for image-guided neurosurgery.
5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression
Authors: Zhao Zheng , Li Liang , Zeng Ruichen , Lin Liangguan , Yuan Dongwei , Wen Yejie , Li Na , Cui Yingying , Zhu Shiming , Zhang Zhi-Min , Li Sheng , Ren Chonghua
Published: December 2023

DNA methylation at the fifth position of cytosine (5-methylcytosine, 5mC) is a crucial epigenetic modification for regulating gene expression, but little is known about how it regulates gene expression in insects. Here, we pursue the detailed molecular mechanism by which DNMT1-mediated 5mC maintenance regulates female reproduction in the German cockroach, Blattella germanica. Our results show that Dnmt1 knockdown decreases the level of 5mC in the ovary, upregulating numerous genes during choriogenesis, especially the transcription factor ftz-f1. The hypomethylation at the ftz-f1 promoter region increases and prolongs ftz-f1 expression in ovarian follicle cells during choriogenesis, which consequently causes aberrantly high levels of 20-hydroxyecdysone and excessively upregulates the extracellular matrix remodeling gene Mmp1. These changes further impair choriogenesis and disrupt fertilization by causing anoikis of the follicle cells, a shortage of chorion proteins, and malformation of the sponge-like bodies. This study significantly advances our understanding of how DNA 5mC modification regulates female reproduction in insects.
A patterned human primitive heart organoid model generated by pluripotent stem cell self-organization
Authors: Volmert Brett , Kiselev Artem , Juhong Aniwat , Wang Fei , Riggs Ashlin , Kostina Aleksandra , O’Hern Colin , Muniyandi Priyadharshni , Wasserman Aaron , Huang Amanda , Lewis-Israeli Yonatan , Panda Vishal , Bhattacharya Sudin , Lauver Adam , Park Sangbum , Qiu Zhen , Zhou Chao , Aguirre Aitor
Published: December 2023

Pluripotent stem cell-derived organoids can recapitulate significant features of organ development in vitro. We hypothesized that creating human heart organoids by mimicking aspects of in utero gestation (e.g., addition of metabolic and hormonal factors) would lead to higher physiological and anatomical relevance. We find that heart organoids produced using this self-organization-driven developmental induction strategy are remarkably similar transcriptionally and morphologically to age-matched human embryonic hearts. We also show that they recapitulate several aspects of cardiac development, including large atrial and ventricular chambers, proepicardial organ formation, and retinoic acid-mediated anterior-posterior patterning, mimicking the developmental processes found in the post-heart tube stage primitive heart. Moreover, we provide proof-of-concept demonstration of the value of this system for disease modeling by exploring the effects of ondansetron, a drug administered to pregnant women and associated with congenital heart defects. These findings constitute a significant technical advance for synthetic heart development and provide a powerful tool for cardiac disease modeling.
Record high room temperature resistance switching in ferroelectric-gated Mott transistors unlocked by interfacial charge engineering
Authors: Hao Yifei , Chen Xuegang , Zhang Le , Han Myung-Geun , Wang Wei , Fang Yue-Wen , Chen Hanghui , Zhu Yimei , Hong Xia
Published: December 2023

The superior size and power scaling potential of ferroelectric-gated Mott transistors makes them promising building blocks for developing energy-efficient memory and logic applications in the post-Moore’s Law era. The close to metallic carrier density in the Mott channel, however, imposes the bottleneck for achieving substantial field effect modulation via a solid-state gate. Previous studies have focused on optimizing the thickness, charge mobility, and carrier density of single-layer correlated channels, which have only led to moderate resistance switching at room temperature. Here, we report a record high nonvolatile resistance switching ratio of 38,440% at 300 K in a prototype Mott transistor consisting of a ferroelectric PbZr0.2Ti0.8O3 gate and an RNiO3 (R: rare earth)/La0.67Sr0.33MnO3 composite channel. The ultrathin La0.67Sr0.33MnO3 buffer layer not only tailors the carrier density profile in RNiO3 through interfacial charge transfer, as corroborated by first-principles calculations, but also provides an extended screening layer that reduces the depolarization effect in the ferroelectric gate. Our study points to an effective material strategy for the functional design of complex oxide heterointerfaces that harnesses the competing roles of charge in field effect screening and ferroelectric depolarization effects.