bluuebunny/crossref_metadata_2025 huggingface dataset:
GENDER AND INVOLVEMENT IN COMMUNITY DEVELOPMENT. A STUDY OF IHIOMA IN ORLU L.G.A, IMO STATE, NIGERIA
Authors: Ukpabi Monday , Ukpabi Monday
Published: June 2021

— This research focused on “men and women in
community development in Ihioma community, in Orlu
local government area State. The broad objective of this
study is to investigate men and women effects in
community development in Ihioma. Two hypothesis were
formulated in the course of this study relevant literature
were reviewed, two theories, were also reviewed, the
theories are modernization theory of social change and
the cultural feminist theory. The researcher adopted the
cultural feminist theory in the explication in Ihioma, the
researcher adopted the survey research design, the
researcher also adopted a sample size of 229 respondents
the purposive sampling method was also adopted by the
researcher. The research gathered data for this research
through the techniques. Data were analyzed using the chisquare statistical tool. The hypothesis which states that
there is no significant relationship between gender
disparity and men and women involvement in community
development was also rejected as the table value of 7.815
was greater than the x2
value of 6.8. The study found out
that gender disparity in Ihioma has not led to a decline in
community development. The study also found out that
the level of education among men and women in Ihioma
has not affected the process of development in the area.
QUALITY ENGINEERING TRANSFORMATION OVER THE YEARS AND ISSUES - A REVIEW
Authors: Subramaniam Pappu Rama , Subramaniam Pappu Rama
Published: June 2021

Traditional quality management and
monitoring has been shown to be unsuccessful. Today,
emerging companies are vying for more value to the
consumer in order to ensure their maximum success and
sustainability. Many businesses want to ensure that their
goods and services are of high quality in order to attract
customers. The current situation is implementing quality
engineering solutions in industries. Quality engineering is
the method of evaluating, handling, designing, and
maintaining various systems in compliance with high
standards. This method ensures that each stage of the
product development cycle is subjected to a thorough
inspection by quality engineers, reducing possible losses by
eliminating defects from the start. Furthermore, highquality maintenance is important and should be made
available for a long time after the product has been
shipped.
Customers' preferences are shifting significantly,
necessitating improvements in design and production
technology, which is becoming increasingly critical in
satisfying individual customers. This necessitates paying
particular attention to quality engineering.
The paper starts with a review on quality emphasis
over the last 37 years, quality concepts, and quality model
evolution followed by i) a contrast of quality management
and quality engineering, ii) developments in quality
engineering tools and techniques, such as statistical
process control (SPC), design of experiment (DoE),
Taguchi processes, and quality function (QFD). This paper
also looks at quality engineering-related problems. There
are brief reviews of recent developments in well-known
quality tools, such as statistical process control, quality
function deployment, and design of experiment. The aim of
this paper is to place quality engineering in context and
emphasise its significance, as well as to present some issues
at the frontiers of quality engineering.
OPTIMAL POWER FLOW USING STATCOM
Authors: Bharol Tanisha , Bora Pranjali , Sai M Sravani , Bansal Dhruvi , Sharma Dr. Sandeep
Published: June 2021

— In power system, active power flow is the main
concern in order to manage the demand supply. The
maximum use of the transmission lines under their
stability limit is very much required. Flexible alternating
current transmission system (FACTS) device are very
much useful to control power system parameters. A
STATCOM (STATCOM) is a FACTS device which is able
to control active and reactive power with voltage
magnitude and phase angle. In this paper, STATCOM is
used in a 10 bus system to control the flow of active power
under contingency condition. Simulation result shows the
effectiveness of the STATCOM in providing the optimal
power flow in the power system considered here.
A STUDY ON SELF-COMPACTING GEOPOLYMER CONCRETE
Authors: Mol J.S Jini , P Dr. Eswaramoorthi
Published: June 2021

In this study aims to demonstrate the
significance of Self-Compacting Geo-Polymer
Concrete (SCGPC) in structures with congested
reinforcement. To achieve good compatibility,
ordinary geopolymer concrete must be compacted with
a lot of vibration. It can be replaced with selfcompacting Geo-Polymer Concrete (SCGPC), which
do not require any type of compaction. Under its own
weight, it fills every corner of the formwork. A less
difference between deformability and stability is
needed for the effective production of self-compacting
geopolymer concrete. Researchers have developed
some guidelines for proportioning self-compacting
geopolymer concrete mixtures, which include i)
lowering the aggregate-to-cementitious-material ratio.
(ii) Raising the volume of the paste and w/c ratio as
well as (iii) using different viscosity-enhancing
admixtures in order to achieve high mobility in selfcompacting geopolymer concrete, superplasticizers are
typically needed. Segregation may be avoided by using
a viscosity changing admixture or a large volume of
powdered content. Fly ash, GGBFS, Silica Fume,
Metakaolin, and slag, among other powdered
materials, may be applied.
Using the Zoom Virtual Platform to Learn Foreign Languages Online
Authors: Kuzikulova D. , Kushimova M. , Sadikova N. , Muratova M.
Published: June 2021

The main aim of this article is to study the effectiveness of using Zoom technology to prepare useful lessons to improve the success of students studying foundation of foreign languages by distance mode at a university. We would like to share our analyze the experience of using the Zoom virtual platform in the process of organizing distance learning from the point of view of the students. The article presents the results of students’ surveys that illustrate the perception of students in terms of the usefulness and ease of use of the utilized software. The article pays particular attention to the estimation of the main characteristics and didactic capabilities of the Zoom virtual platform for organizing distance learning foreign language classes at a university. The article draws conclusions on the advantages and disadvantages of the software, describes the stages of work and offers methodological recommendations as well as lists the main difficulties that teachers and students at Tashkent State University of Economics have encountered in the course of language education.
Competence Methods of Learning Songs in Elementary School on the Subject “Literary Reading”
Authors: Akmatova Ch.
Published: June 2021

The subject of this research is the activity of teaching children’s songs on the subject “Literary reading”. The aim is to test the effectiveness of the evidence-based model in schools and present proven options to primary school teachers. Theoretical, empirical and experimental methods were used in research. In the course of the experimental work, it was shown that the views and attitudes of students and teachers to teaching children’s songs in the subject “Literary reading” were carried out by pedagogical research methods and the obtained results. The methods of teaching in Soviet schools are analyzed depending on the type of work. The peculiarity of the results was that the plot and satirical works were not remembered for a long time and in a humorous and satirical form they revealed the character of people in the works. As a result of experimental activity, a model of teaching children’s song in primary school was demonstrated. As a result, in order to increase the effectiveness of the lesson in teaching competence-based songs for children, teachers were asked to focus on the areas of competence-based teaching methods, modern pedagogical technologies and tools in the teaching process.
The Essence and Content of Socialization of Preschool Children
Authors: Kaldybaeva A. , Sabirova M.
Published: June 2021

The article discusses the problems of socialization of children, the essence and content of the concept of socialization, social education in the family, its basics and modern mechanisms for effective education of preschool children. The real potential of the ethnocultural potential of society in the socialization of preschool children was also considered including features of society in relation to social and ethnocultural phenomena and peculiarities of ethnic traditions of social “education and development” of children from an early age. Social education of the personality of a preschooler considered in a broad and narrow sense, systems of interaction between the family and the preschool educational institution and the possibilities of improving the conditions of pedagogical work of educators with increasing the efficiency of the socialization process by improving the professionalism of specialists.
Family as a Primary Socialization in Preschool Children Upbringing
Authors: Sabirova M.
Published: June 2021

This article examines the role of the family and its features in the process of socialization, also the main forms and functions (informative, mentor function, initiating and orienting function) of the family. Effective mechanisms of family social education of a child were described including the psychological climate of the family and its factors: national traditions, moral values, educational level, etc. Features of children's folklore in education. Methods of accustoming and the totality of the views of the people. The main components of the rural ethnocultural space.
Formation of Tolerant Competence of Junior Classes on the Basis of Folk Pedagogy
Authors: Akmatova
Published: June 2021

The article examines the meaning and significance of tolerance in human life and behavior, as well as the formation of tolerant competencies in junior schoolchildren on the basis of folk pedagogy. The studies were conducted by researchers who consider tolerance to be an important personality value. The functions and types of tolerance in society have been identified. According to the results, it was found that in the process of teaching children's songs in elementary school, using the rich experience of folk pedagogy, students have an excellent opportunity to develop ethnocultural competence, including tolerance.
Educational Importance of Tales in Primary Schools and Teaching Methods
Authors: Sherbaeva Zh.
Published: June 2021

The article deals with the formation of a humane worldview, an active life position, moral faith, spiritual culture, the development of oral and written speech by acquainting schoolchildren with the best examples of life-tested folklore treasures.
The Introduction of a Credit-module System of Education in Uzbekistan Higher Educational Institutions
Authors: Dadakhonova Z.
Published: June 2021

The article deals with the introduction of credit-module system, which is an important factor in stimulating the effective work of teachers and students. The author of the article reveals the concept of module and credit. Modular training is the most coherent, clear and effective technology of education, which guarantees the quality of training of competent specialists. Specialists trained under the programs created by modular technology, possess not only the knowledge, but also the skills of the chosen profession and specialty: decision-making, performance of services and production work.
Teaching Methods of English for Specific Purposes
Authors: Saidvalieva D.
Published: June 2021

Foreign Language Teachers for Specific Purposes have a lot in common with teachers of general foreign language. For both it is necessary to consider linguistic development and teaching theories, to have insights in contemporary ideas regarding their own position and role as well as the position and role of foreign language learners in education and to face new technologies offered as an aid to improve their methodology. The most important difference lies in the learners and their purposes for learning English. ESP students are usually adults who already have some acquaintance with English and are learning the language in order to communicate a set of professional skills and to perform particular job-related functions. An ESP program is therefore built on an assessment of purposes and needs and the functions for which English is required.
Active Teaching Methods as Means of Developing Students’ Creativity
Authors: Bylykova M.
Published: June 2021

In the article, you can get an answer to such questions as the place of a lecture in education and on the basis of this concept about the content of a lecture, what can and cannot be talked about. You cannot give all the material in a lecture. In fact, both an inexperienced teacher and a teacher with vast experience can make one methodological mistake: they want to include all their knowledge in the lecture. The teacher, trying to have time to present all the prepared material, lectures quickly. This of course reduces the effectiveness of the lecture. This note provides specific suggestions and discusses the didactic and psychological conditions for organizing the lecture. This article is recommended for university professors for use in lectures.
Transportation Problems of Karakalpakstan in 1920-1930s
Authors: Matjanov A.
Published: June 2021

The article analyzes the state of the national economy of Karakalpakstan in the 1920–1930s. It is emphasized that at this time transport problems arose in front of the Republic for the development of the national economy. A historical excursion to the history of traditional transport among the peoples of the lower reaches of the Amu Darya is carried out. It is noted that before industrialization it was necessary to solve transport problems. Some concepts of scientists on the development of transport in Karakalpakstan are analyzed.
Pelatihan Manajemen dan Kepemimpinan Karang Taruna Kelurahan Pamulang Timur Kecamatan Pamulang Tangerang Selatan
Authors: Putranto Ivan , Eliyani Citra , Syamruddin Syamruddin , Yulianti Rr Mardiana , Widodo Sugeng
Published: June 2021

Tujuan Pengabdian Kepada Masyarakat ini adalah sebagai bentuk aplikasi Tri Darma Perguruan Tinggi, silaturahmi antar dosen, pengurus, anggota Karang Taruna Kelurahan Pamulang Timur dan aparatur pemerintah khususnya Kelurahan, menggali potensi/minat pemuda dalam menjadi seorang pimpinan organisasi, Melatih kepada pemuda dalam menjadi seorang pimpinan di organisasi. Metode dalam pengandian masyarakat yang dilakukan di Karang Taruna Kelurahan Pamulang Timur dengan menggunakan metode dalam bentuk pelatihan keterampilan melalui ceramah, demontrasi dan tanya jawab. Adapun tahapan-tahapan dalam pelaksanaan kegiatannya: ceramah digunakan untuk menyampaikan pengetahuan secara umum tentang manejemen dan kepemimpinan dalam organisasi Karang Taruna, organisasi yang efektif, Kepemimpinan di dalam organisasi dan pengambilan keputusan dalam organisasi, demontrasi digunakan untuk memberikan keterampilan langsung mengenai proses menejemen dan kepemimpinan dalam organisasi Karang Taruna, tanya jawab digunakan untuk melengkapi hal-hal yang belum terakomodasi oleh kedua metode diatas, game/Pelatihan menejemen dan kepemimpinan dalam organisasi Karang Taruna seluruh peserta pelatihan, evaluasi hasil akhir. Hasil pengabdian yang diperoleh adalah menambah pengetahuan dan kompetensi di dalam berorganisasi. Antusiasme peserta dan pihak pemerintah setempat atau lurah dan jajarannya yang sangat akomodatif dalam menyambut rencana pelatihan hingga dilaksanakannya kegiatan pelatihan memberikan motivasi yang sangat besar bagi dosen-dosen Unpam yang melaksanakan kegiatan Pengabdian Kepada Masyarakat (PKM) di Karang Taruna Kelurahan Pamulang Timur.
Penyuluhan Pemanfaatan Limbah Buah Menjadi Pupuk Cair Organik Untuk Memenuhi Kebutuhan Petani Buah Palawija dan Sayuran Dalam Meningkatkan Pendapatan Keluarga di Desa Karang Dadap Kecamatan Kalibagor, Banyumas – Jawa Tengah
Authors: Dewi Iriana Kusuma , Rahman Ana Septia , Kristina Elizabeth Tika , Septiani Yeni , Jamaludin Pusporini Palupi
Published: June 2021

Tujuan dari Kegiatan Pengabdian Kepada Masyarakat adalah untuk melaksanakan salah satu Tri Darma Perguruan Tinggi khususnya di Universitas Pamulang. Selain itu diharapkan dengan pengabdian kepada masyarakat tersebut keberadaan perguruan tinggi dapat memberikan kontribusi kepada pengembangan penerapan keilmuan kepada masyarakat. Metode yang digunakan pada pengabdian kepada masyarakat ini penyampaikan materi secara verbal dalam pelaksanaan ini materi dalam penyuluhan berisi tentang bagaimana cara untuk mengetahui manfaat lain dari limbah buah, serta potensi yang bisa diperoleh dari limbah buah tersebut. Sehingga masyarakat juga bisa mengelola sendiri pupuk yang akan mereka pergunakan. Hasil pengabdian masyarakat yang diperoleh adalah kegiatan yang dilaksanakan untuk masyarakat Desa Karang Dadap adalah kegiatan untuk mengatasi persoalan limbah buah yang banyak dibuang di lingkungan sekitar yaitu toko-toko penjual buah-buahan ataupun dipasar-pasar. Penggunaan pupuk cair organik dari limbah buah sangat bermanfaat bagi para petani palawija dan dapat mengurangi pengunaan pupuk kimia yang dapat merusak tanah. Selain itu, limbah buah-buahan bisa menjadi pupuk organik cair berpotensi secara ekonomi karena dapat dijual kepada para petani buah, palawija dan sayuran dengan harga yang terjangkau akan tetapi dengan kualitas pupuk yang baik. Ilmu yang diperoleh pada pengabdian masyarakat ini harapannya semakin memberikan wawasan mengenai pemanfaatan penggunaan limbah buah yang terdapat di lingkungan sekitar untuk memenuhi kebutuhan petani buah palawija dan sayuran dalam meningkatkan pendapatan keluarga khususnya di Desa Karang Dadap, Kecamatan Kalibagor, Banyumas – Jawa Tengah.
Manajemen Kewirausahaan Sosial Suatu Alternatif Pada PAC GP Ansor Kecamatan Cipondoh Kota Tangerang
Authors: Sholeh Achmad Nur , Nazir Ahmad , Nizar Nefo Indra , Nasution Ahmad Yani , Abdurohman Dede
Published: June 2021

Tujuan dilakukannya pengabdian masyarakat ini adalah: untuk mengetahui bagaimana keterlibatan PAC GP Ansor Cipondoh dalam kewirausahaan sosial dan Bagaimana untuk mengetahui menjalankan manajemen kewirausahaan soaial pada PAC GP Ansor Cipondoh. Kerangka pemecahan masalah yang telah dirumuskan kemudian direalisasikan oleh tim pengabdi. Beberapa tindakan dalam upaya merealisasikan pemecahan permasalahan tersebut dilakukan dengan 1. Ceramah dan tanya jawab tentang menejemen kewirausahaan sosial dilaksanakan pada tanggal 12 juni 2020 2. Pelatihan teknis kewirausahaan pada era industry 4.0 dilaksanakan pada tanggal 13 juni 2020 3. Pendampingan dalam berwirausahaan pada era industry 4.0 dilaksanakan pada tanggal 14 juni 2020. Pengabdian kepada masyarakat ini dilakukan pada PAC GP Ansor Cipondoh beralamat di Jl Ki Hajar Dewantoro Rt 05/02 Kelurahan Gondrong Kecamatan Cipondoh Kota Tangerang Banten. Dilakukan pada hari jumat sampai minggu tanggal 12 sampai 14 juni 2020. Pendampingan dilakukan secara kelompok. Peserta yang mengikuti pengabdian ini adalah pengurus dan anggota PAC GP Ansor Cipondoh berjumlah 45 orang. Adapun hasil yang didapatkan di dalam pengabdian ini Keterlibatan PAC GP Ansor Cipondoh dalam kewirausahaan soaial dilakukan dengan melakukan kegiatan-kegiatan yang bersentuhan langsung dengan kebutuhan para pengurus, anggota dan masyarakat sekitar dan Menejemen kewirausahaan sosial yang dilakukan oleh pada PAC GP Ansor Cipondoh masih belum maksimal, karena terkendala oleh beberapa hal, baik teknis maupun non teknis di lapangan terutama di dalam bidang sumber daya manusia yaitu yang berkaitan dengan pengelolaan.
Pengaruh Return on Asset dan NonPerforming Loan Terhadap Capital Adequacy Ratio Pada PT Bank Mega Tbk
Authors: Faisal Faisal
Published: June 2021

This study aims to determine the effect of the independent variables on the dependent variable. The independent variable used in this study is Return on Assets, Non Performing Loans. While the dependent variable in this study is the Capital Adequacy Ratio. The data used in this study are quarterly data from 2010 to 2018. The sampling technique used in this study was purposive sampling involving Bank Mega Tbk. The analysis technique used is multiple linear regression analysis. Based on the results of data analysis, this study shows that simultaneously (Simultaneous) Return on Assets, Non Performing Loans have a significant effect on Capital Adequacy Ratio. And individually (partial) Net Interest Margin, Non Performing Loans and Return on Assets have a positive effect on CAR, negatively affect CAR. Determination coefficient results indicate the Adjusted R-squared value of 0.714240 means that the independent variable can explain the dependent variable by 71.42% while the rest is explained by other variables not contained in the model.In this study, the researcher wanted to find out whether there was a significant relationship between Return on Assets, Non-Performing Loans had a significant effect on the Capital Adequacy Ratio.

 

Abstrak

Penelitian ini bertujuan untuk mengetahui pengaruh antara variabel independen terhadap variabel dependen. Variabel independen yang digunakan dalam penelitian ini adalah Return on Asset, Non Performing Loan. Sedangkan variabel dependen dalam penelitian ini adalah Capital Adequacy Ratio. Data yang digunakan dalam penelitian ini adalah data kuartal selama periode 2010 sampai dengan 2018. Teknik sampling yang digunakan dalam penelitian ini adalah purposive sampling yang melibatkan Bank Mega Tbk. Teknik analisis yang digunakan adalah analisis regresi linear berganda. Berdasarkan hasil analisis data penelitian ini menunjukan secara bersama-sama (Simultan) Return on Asset, Non Performing Loan berpengaruh signifikan terhadap Capital Adequacy Ratio. Dan secara individu (Parsial) Net Interest Margin, Non Performing Loan dan Return on Asset berpengaruh positif terhadap CAR, berpengaruh negatif terhadap CAR. Hasil Koefisien Determinasi menunjukan nilai Adjusted R-squared sebesar 0,714240 artinya bahwa variabel independen dapat menjelaskan variabel dependen sebesar 71,42% sedangkan sisanya dijelaskan oleh variabel lain yang tidak terdapat dalam model.Return on Asset, Non Performing Loan berpengaruh signifikan terhadap Capital Adequacy Ratio

 

KataKunci: Return on Asset, Non Performing Loan, Capital Adequacy Ratio.
Analisis Evaluasi Keberhasilan Inovasi Sistem Aplikasi By Data Pada UKM Minuman Kekinian di Kota Tangerang Selatan(Studi Kasus Minuman "Ngombe”)
Authors: Tumanggor Rahmayanti , Wiyanto Wiyanto
Published: June 2021

This aims of the study is to analyze the evaluation of innovation successful of by data application system at modern beverage MSMEs (Ngombe) in South Tangerang City. Also to find out influence and benefits of using by data application system to increase sales of modern beverages. Alsoto know how the “by data” application system can help modern beverage business entrepreneursto achieve significant sales and to provide economic benefits.The method used in this study was descriptive analysis of qualitative method. The analysis technique uses observation, interview, documentation, forum group discussion and triangulation. The analysis data method was inductive that based on the accumulation of data, then developed in to hypothesis by organize the data in to category, outline into units, do synthesis, compile in to the pattern to get the conclusion. Based on the SWOT analysis, the EFI and EFE calculations obtained the EFI score of 3.65, meaning that the business has a good internal strength position. While the EFE score is 3.30 indicating that external factors are sufficient to support the business. By using the SPACE matrix, Ngombe is in quadrant III, which is an aggressive condition. The conclusion is that implementation of by data application system gives ease in the service to consumers and provides significant advantage to the owner.

Abstrak

Penelitian ini bertujuan untuk menganalisis evaluasi keberhasilan inovasi sistem aplikasi by data pada UKM minuman kekinian (Ngombe) di Kota Tangerang Selatan. Juga untuk mengetahui pengaruh dan manfaat penggunaan sistem aplikasi by data terhadap peningkatan penjualan minuman kekinian. Bagaimana sistem aplikasi ‘by data” dapat membantu para pelaku usaha minuman kekinian dalam mengembangkan usahanya sehingga tercapai penjualan yang cukup signifikan dan memberi keuntungan ekonomis Metode penelitian yang digunakan adalah kualitatif yang bersifat analisis deskriptif.Teknik pengumpulan data adalah observasi, wawancara, dokumentasi, FGD dan triangulasi. Metode analisis data bersifat induktif, dikembangkan menjadi hipotesis, mengorganisasikan data ke dalam kategori, menjabarkan ke dalam unit-unit, melakukan sintesa, menyusun ke dalam pola, sehingga mendapatkan satu kesimpulan. Hasil penelitian berdasarkan analisis SWOT perhitunganan EFI dan EFE nilai skor tertimbang EFI sebesar 3.65, artinya usaha yang dikembangkan memiliki posisi kekuatan internal baik. Sedangkan skor tertimbang EFE diperoleh nilai 3,30 mengindikasikan bahwa faktor eksternal cukup mendukung usaha yang dilakukan. Denganmenggunakanmatrik SPACE,usaha Minuman Ngombe berada padaposisi kuadran III yaitu kondisi agresif.Kesimpulannya bahwa minuman kekinian yang menggunakan sistem aplikasi‘by data’memberikan kemudahan dalam penjualan, juga memberikan keuntungan yang signifikan bagi pemiliknya.

 

Kata Kunci:Produk, Profit, Inovasi, Sistem Aplikasi by data
Ultra-Thin Porous PDLLA Films Promote Generation, Maintenance, and Viability of Stem Cell Spheroids
Authors: Tsai Ya An , Li Tianshu , Torres-Fernández Lucia A. , Weise Stefan C. , Kolanus Waldemar , Takeoka Shinji
Published: June 2021

Three-dimensional (3D) culture bridges and minimizes the gap between in vitro and in vivo states of cells and various 3D culture systems have been developed according to different approaches. However, most of these approaches are either complicated to operate, or costive to scale up. Therefore, a simple method for stem cell spheroid formation and preservation was proposed using poly(D,L-lactic acid) porous thin film (porous nanosheet), which were fabricated by a roll-to-roll gravure coating method combining a solvent etching process. The obtained porous nanosheet was less than 200 nm in thickness and had an average pore area of 6.6 μm2 with a porosity of 0.887. It offered a semi-adhesive surface for stem cells to form spheroids and maintained the average spheroid diameter below 100 μm for 5 days. In comparison to the spheroids formed in suspension culture, the porous nanosheets improved cell viability and cell division rate, suggesting the better feasibility to be applied as 3D culture scaffolds.
Comprehensive Analysis Reveals the Potential Regulatory Mechanism Between Ub–Proteasome System and Cell Cycle in Colorectal Cancer
Authors: Zhang Zhiyuan , Chen Jingwen , Tang Wentao , Feng Qingyang , Xu Jianmin , Ren Li
Published: June 2021

The ubiquitin (Ub)–proteasome system (UPS) is an important regulatory component in colorectal cancer (CRC), and the cell cycle is also characterized to play a significant role in CRC. In this present study, we firstly identified UPS-associated differentially expressed genes and all the differentially expressed protein-coding genes in CRC through three differential analyses. UPS-associated genes were also further analyzed via survival analysis. A weighted gene co-expression network analysis (WGCNA) was used to identify the cell cycle-associated genes. We used protein–protein interaction (PPI) network to comprehensively mine the potential mechanism of the UPS–cell cycle regulatory axis. Moreover, we constructed a signature based on UPS-associated genes to predict the overall survival of CRC patients. Our research provides a novel insight view of the UPS and cell cycle system in CRC.
WIP, YAP/TAZ and Actin Connections Orchestrate Development and Transformation in the Central Nervous System
Authors: Antón Inés M. , Wandosell Francisco
Published: June 2021

YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are transcription co-regulators that make up the terminal components of the Hippo signaling pathway, which plays a role in organ size control and derived tissue homeostasis through regulation of the proliferation, differentiation and apoptosis of a wide variety of differentiated and stem cells. Hippo/YAP signaling contributes to normal development of the nervous system, as it participates in self-renewal of neural stem cells, proliferation of neural progenitor cells and differentiation, activation and myelination of glial cells. Not surprisingly, alterations in this pathway underlie the development of severe neurological diseases. In glioblastomas, YAP and TAZ levels directly correlate with the amount of the actin-binding molecule WIP (WASP interacting protein), which regulates stemness and invasiveness. In neurons, WIP modulates cytoskeleton dynamics through actin polymerization/depolymerization and acts as a negative regulator of neuritogenesis, dendrite branching and dendritic spine formation. Our working hypothesis is that WIP regulates the YAP/TAZ pools using a Hippo-independent pathway. Thus, in this review we will present some of the data that links WIP, YAP and TAZ, with a focus on their function in cells from the central and peripheral nervous systems. It is hoped that a better understanding of the mechanisms involved in brain and nervous development and the pathologies that arise due to their alteration will reveal novel therapeutic targets for neurologic diseases.
Epigenetic Regulation of Autophagy Beyond the Cytoplasm: A Review
Authors: Shi Yin , Shen Han-Ming , Gopalakrishnan Vidya , Gordon Nancy
Published: June 2021

Autophagy is a highly conserved catabolic process induced under various stress conditions to protect the cell from harm and allow survival in the face of nutrient- or energy-deficient states. Regulation of autophagy is complex, as cells need to adapt to a continuously changing microenvironment. It is well recognized that the AMPK and mTOR signaling pathways are the main regulators of autophagy. However, various other signaling pathways have also been described to regulate the autophagic process. A better understanding of these complex autophagy regulatory mechanisms will allow the discovery of new potential therapeutic targets. Here, we present a brief overview of autophagy and its regulatory pathways with emphasis on the epigenetic control mechanisms.
A Voltammetric Perspective of Multi-Electron and Proton Transfer in Protein Redox Chemistry: Insights From Computational Analysis of Escherichia coli HypD Fourier Transformed Alternating Current Voltammetry
Authors: Dale-Evans Alister R. , Robinson Martin J. , Lloyd-Laney Henry O. , Gavaghan David J. , Bond Alan M. , Parkin Alison
Published: June 2021

This paper explores the impact of pH on the mechanism of reversible disulfide bond (CysS-SCys) reductive breaking and oxidative formation inEscherichia colihydrogenase maturation factor HypD, a protein which forms a highly stable adsorbed film on a graphite electrode. To achieve this, low frequency (8.96 Hz) Fourier transformed alternating current voltammetric (FTACV) experimental data was used in combination with modelling approaches based on Butler-Volmer theory with a dual polynomial capacitance model, utilizing an automated two-step fitting process conducted within a Bayesian framework. We previously showed that at pH 6.0 the protein data is best modelled by a redox reaction of two separate, stepwise one-electron, one-proton transfers with slightly “crossed” apparent reduction potentials that incorporate electron and proton transfer terms (Eapp20>Eapp10). Remarkably, rather than collapsing to a concerted two-electron redox reaction at more extreme pH, the same two-stepwise one-electron transfer model withEapp20>Eapp10continues to provide the best fit to FTACV data measured across a proton concentration range from pH 4.0 to pH 9.0. A similar, small level of crossover in reversible potentials is also displayed in overall two-electron transitions in other proteins and enzymes, and this provides access to a small but finite amount of the one electron reduced intermediate state.
Characterization of the Tubovesicular Network in Plasmodium vivax Liver Stage Hypnozoites and Schizonts
Authors: Sylvester Kayla , Maher Steven P. , Posfai Dora , Tran Michael K. , Crawford McKenna C. , Vantaux Amélie , Witkowski Benoît , Kyle Dennis E. , Derbyshire Emily R.
Published: June 2021

Plasmodium is a genus of apicomplexan parasites which replicate in the liver before causing malaria. Plasmodium vivax can also persist in the liver as dormant hypnozoites and cause clinical relapse upon activation, but the molecular mechanisms leading to activation have yet to be discovered. In this study, we use high-resolution microscopy to characterize temporal changes of the P. vivax liver stage tubovesicular network (TVN), a parasitophorous vacuole membrane (PVM)-derived network within the host cytosol. We observe extended membrane clusters, tubules, and TVN-derived vesicles present throughout P. vivax liver stage development. Additionally, we demonstrate an unexpected presence of the TVN in hypnozoites and observe some association of this network to host nuclei. We also reveal that the host water and solute channel aquaporin-3 (AQP3) associates with TVN-derived vesicles and extended membrane clusters. AQP3 has been previously shown to localize to the PVM of P. vivax hypnozoites and liver schizonts but has not yet been shown in association to the TVN. Our results highlight host-parasite interactions occur in both dormant and replicating liver stage P. vivax forms and implicate AQP3 function during this time. Together, these findings enhance our understanding of P. vivax liver stage biology through characterization of the TVN with an emphasis on the presence of this network in dormant hypnozoites.
Pre-ischemic Lactate Levels Affect Post-ischemic Recovery in an Isolated Rat Heart Model of Donation After Circulatory Death (DCD)
Authors: Arnold Maria , Segiser Adrian , Graf Selianne , Méndez-Carmona Natalia , Sanz Maria N. , Wyss Rahel K. , Kalbermatter Nina , Keller Nino , Carrel Thierry , Longnus Sarah
Published: June 2021

Introduction: Donation after circulatory death (DCD) could substantially improve donor heart availability. In DCD, the heart is not only exposed to a period of warm ischemia, but also to a damaging pre-ischemic phase. We hypothesized that the DCD-relevant pre-ischemic lactate levels negatively affect the post-ischemic functional and mitochondrial recovery in an isolated rat heart model of DCD.

Methods: Isolated, working rat hearts underwent 28.5′ of global ischemia and 60′ of reperfusion. Prior to ischemia, hearts were perfused with one of three pre-ischemic lactate levels: no lactate (0 Lac), physiologic lactate (0.5 mM; 0.5 Lac), or DCD-relevant lactate (1 mM; 1 Lac). In a fourth group, an inhibitor of the mitochondrial calcium uniporter was added in reperfusion to 1 Lac hearts (1 Lac + Ru360).

Results: During reperfusion, left ventricular work (heart rate-developed pressure product) was significantly greater in 0.5 Lac hearts compared to 0 Lac or 1 Lac. In 1 vs. 0.5 Lac hearts, in parallel with a decreased function, cellular and mitochondrial damage was greater, tissue calcium content tended to increase, while oxidative stress damage tended to decrease. The addition of Ru360 to 1 Lac hearts partially abrogated the negative effects of the DCD-relevant pre-ischemic lactate levels (greater post-ischemic left ventricular work and less cytochrome c release in 1 Lac+Ru360 vs. 1 Lac).

Conclusion: DCD-relevant levels of pre-ischemic lactate (1 mM) reduce contractile, cellular, and mitochondrial recovery during reperfusion compared to physiologic lactate levels. Inhibition of mitochondrial calcium uptake during early reperfusion improves the post-ischemic recovery of 1 Lac hearts, indicating calcium overload as a potential therapeutic reperfusion target for DCD hearts.
Plasma Testosterone Levels and Atherosclerotic Plaque Gene Expression in Men With Advanced Atherosclerosis
Authors: Groepenhoff Floor , Diez Benavente Ernest , Boltjes Arjan , Timmerman Nathalie , Waissi Farahnaz , Hartman Robin J. G. , Onland-Moret N. C. , Pasterkamp Gerard , Den Ruijter Hester
Published: June 2021

Aims: Low plasma testosterone levels have been shown to predict worse outcome in men with severe atherosclerotic disease. We hypothesized that a low plasma testosterone level affects disease risk through changes in gene expression in atherosclerotic plaques. Therefore, we studied plasma testosterone levels in relation to gene expression levels in atherosclerotic plaque tissue of men with advanced atherosclerotic disease.

Methods: Plasma testosterone levels were measured in 203 men undergoing carotid endarterectomy. The corresponding atherosclerotic plaque tissue was used for RNA sequencing. First, we assessed how often the androgen receptor gene was expressed in the plaque. Second, correlations between plasma testosterone levels and pre-selected testosterone-sensitive genes were assessed. Finally, differences within the RNA expression profile of the plaque as a whole, characterized into gene regulatory networks and at individual gene level were assessed in relation to testosterone levels.

Results: Testosterone plasma levels were low with a median of 11.6 nmol/L (IQR: 8.6–13.8). RNA-seq of the plaque resulted in reliable expression data for 18,850 genes to be analyzed. Within the RNA seq data, the androgen-receptor gene was expressed in 189 out of 203 (93%) atherosclerotic plaques of men undergoing carotid endarterectomy. The androgen receptor gene expression was not associated with testosterone plasma levels. There were no significant differences in gene expression of atherosclerotic plaques between different endogenous testosterone levels. This remained true for known testosterone-sensitive genes, the complete transcriptomic profile, male-specific gene co-expression modules as well as for individual genes.

Conclusion: In men with severe atherosclerotic disease the androgen receptor is highly expressed in plaque tissue. However, plasma testosterone levels were neither associated with pre-selected testosterone sensitive genes, gene expression profiles nor gene regulatory networks in late-stage atherosclerotic plaques. The effect of testosterone on gene expression of the late-stage atherosclerotic plaque appears limited, suggesting that alternate mechanisms explain its effect on clinical outcomes.
Reservoir-Triggered Earthquakes Around the Atatürk Dam (Southeastern Turkey)
Authors: Büyükakpınar Pınar , Cesca Simone , Hainzl Sebastian , Jamalreyhani Mohammadreza , Heimann Sebastian , Dahm Torsten
Published: June 2021

Reservoir-triggered seismicity has been observed near dams during construction, impoundment, and cyclic filling in many parts of the earth. In Turkey, the number of dams has increased substantially over the last decade, with Atatürk Dam being the largest dam in Turkey with a total water capacity of 48.7 billion m3. After the construction of the dam, the monitoring network has improved. Considering earthquakes above the long-term completeness magnitude of MC= 3.5, the local seismicity rate has substantially increased after the filling of the reservoir. Recently, two damaging earthquakes of Mw5.5 and Mw5.1 occurred in the town of Samsat near the Atatürk Reservoir in 2017 and 2018, respectively. In this study, we analyze the spatio-temporal evolution of seismicity and its source properties in relation to the temporal water-level variations and the stresses resulting from surface loading and pore-pressure diffusion. We find that water-level and seismicity rate are anti-correlated, which is explained by the stabilization effect of the gravitational induced stress imposed by water loading on the local faults. On the other hand, we find that the overall effective stress in the seismogenic zone increased over decades due to pore-pressure diffusion, explaining the enhanced background seismicity during recent years. Additionally, we observe a progressive decrease of the Gutenberg-Richter b-value. Our results indicate that the stressing rate finally focused on the region where the two damaging earthquakes occurred in 2017 and 2018.
Multi—Proxy Reconstructions of Climate Change and Human Impacts Over the Past 7000 Years From an Archive of Continental Shelf Sediments off Eastern Hainan Island, China
Authors: Huang Chao , Kong Deming , Chen Fajin , Hu Jianfang , Wang Peng , Lin Junchuan
Published: June 2021

Abrupt climatic events and the history of human activities on Hainan Island are poorly understood, due to the lack of high-resolution records. We present high-resolution multiproxy records from the coastal shelf off eastern Hainan Island in China to investigate abrupt climate change and regional human–environment interaction over the last 7,000 years. A prominent climatic anomaly occurred during 5,400–4,900 cal yr BP. This abrupt monsoon failure has been detected in various paleoclimatic records from monsoonal regions. Anomalous summer monsoon intensity during 5,400–4,900 cal yr BP is probably driven by solar variability, ENSO activity and ice-rafting events in the North Atlantic. Over the past 1,500 years, with the growing population and progress in production technology, human activity has increasingly become the dominant factor controlling the natural environment of Hainan Island.
SNAPIN Regulates Cell Cycle Progression to Promote Pancreatic β Cell Growth
Authors: Jiang Mengxue , Kuang Zhijian , He Yaohui , Cao Yin , Yu Tingyan , Cheng Jidong , Liu Wen , Wang Wei
Published: June 2021

In diabetes mellitus, death of β cell in the pancreas occurs throughout the development of the disease, with loss of insulin production. The maintenance of β cell number is essential to maintaining normoglycemia. SNAPIN has been found to regulate insulin secretion, but whether it induces β cell proliferation remains to be elucidated. This study aimed to explore the physiological roles of SNAPIN in β cell proliferation. SNAPIN expression increases with the age of mice and SNAPIN is down-regulated in diabetes. KEGG pathway and GO analysis showed that SNAPIN- interacting proteins were enriched in cell cycle regulation. B cell cycle was arrested in the S phase, and cell proliferation was inhibited after SNAPIN knockdown. The expression of CDK2, CDK4 and CCND1 proteins in the S phase of the cell cycle were reduced after SNAPIN knockdown, whereas they were increased after overexpression of SNAPIN. In addition, insulin protein and mRNA levels also increased or decreased after SNAPIN knockdown or overexpression, respectively. Conclusions: Our data indicate that SNAPIN mediates β cells proliferation and insulin secretion, and provide evidences that SNAPIN might be a pharmacotherapeutic target for diabetes mellitus.
Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
Authors: Godoi Filipe G. Andrade , Forner-Piquer Isabel , Randazzo Basilio , Habibi Hamid R. , Lo Nostro Fabiana L. , Moreira Renata Guimarães , Carnevali Oliana
Published: June 2021

Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.
Opsin Evolution in Flower-Visiting Beetles
Authors: Sharkey Camilla R. , Powell Gareth S. , Bybee Seth M.
Published: June 2021

Flowers have evolved signals that exploit the sensory systems of insect visitors. In the case of visual cues, color signals are thought to have been shaped in large part by the spectral sensitivity of key pollinators, such as hymenopterans. Beetles were some of the first plant pollinators, pre-dating the angiosperm radiation but with the exception of a few well-studied species, the evolution of flower-visiting beetle visual systems is poorly understood. Thus, the ability of beetles to detect and distinguish flower color signals and perhaps their potential role in shaping flower coloration is not well understood. Traditional models of pollinator visual systems often assume a putative tri- or tetrachromatic flower-visitor, as is found in bees, flies and butterflies. Beetles are unique among modern pollinators as ancestrally they did not possess the machinery for trichromatic vision, lacking the blue-sensitive photoreceptor class. Research on the evolution of visual genes responsible for wavelength sensitivity (opsins) has revealed that beetles with putative tri- and tetrachromatic visual systems have evolved independently, along multiple lineages. We explore the evolution of beetle visual genes using newly generated and publicly available RNA-seq data from 25 species with flower associations, including previously unexplored key flower-visitor groups and 20 non-flower visiting relatives. Our findings serve as a resource to inform and guide future studies on beetle-flower interactions, where insight from both signal and receiver is needed to better understand these poorly explored systems.