bluuebunny/crossref_metadata_2025 huggingface dataset:
Early effects of androgen deprivation on bone and mineral homeostasis in adult men: a prospective cohort study
Authors: Khalil Rougin , Antonio Leen , Laurent Michaël R , David Karel , Kim Na Ri , Evenepoel Pieter , Eisenhauer Anton , Heuser Alexander , Cavalier Etienne , Khosla Sundeep , Claessens Frank , Vanderschueren Dirk , Decallonne Brigitte
Published: May 2020

Objective

Long-term androgen deprivation therapy (ADT) negatively influences bone. The short-term effects on bone and mineral homeostasis are less known. Therefore, we aimed to investigate the early effects of ADT on calcium/phosphate homeostasis and bone turnover.

Design

Prospective cohort study.

Methods

Eugonadal adult, male sex offenders, who were referred for ADT to the endocrine outpatient clinic, received cyproterone acetate. Changes in blood markers of calcium/phosphate homeostasis and bone turnover between baseline and first follow-up visit were studied.

Results

Of 26 screened patients, 17 were included. The median age was 44 (range 20–75) years. The median time interval between baseline and first follow-up was 13 (6–27) weeks. Compared to baseline, an 81% decrease was observed for median total testosterone (to 3.4 nmol/L (0.4–12.2); P < 0.0001) and free testosterone (to 0.06 nmol/L (0.01–0.18); P < 0.0001). Median total estradiol decreased by 71% (to 17.6 pmol/L (4.7–35.6); P < 0.0001). Increased serum calcium (P < 0.0001) and phosphate (P = 0.0016) was observed, paralleled by decreased PTH (P = 0.0156) and 1,25-dihydroxyvitamin D3 (P = 0.0134). The stable calcium isotope ratio (δ44/42Ca) decreased (P = 0.0458), indicating net calcium loss from bone. Bone-specific alkaline phosphatase and osteocalcin decreased (P < 0.0001 and P = 0.0056, respectively), periostin tended to decrease (P = 0.0500), whereas sclerostin increased (P < 0.0001), indicating suppressed bone formation. Serum bone resorption markers (TRAP, CTX) were unaltered.

Conclusions

In adult men, calcium release from the skeleton occurs early following sex steroid deprivation, reflecting early bone resorption. The increase of sclerostin and reduction of bone formation markers, without changes in resorption markers, suggests a dominant negative effect on bone formation in the acute phase.
Prophylactic treatment of ischemic stroke with Coffea arabica in rats: A preliminary study
Authors: Khanum Arshia Batool , Shakir Lubna , -ur-Rehman Zaka , Khan Tanveer Ahmed , Najam Komal , Saeed Nasira , Khan Mahtab Ahmed , Nazeer Anam , Nazir Tayyaba , Aslam Shawana
Published: May 2020

Introduction: Ischemic stroke is the third leading cause of death worldwide. The purpose of the current study was to assess the preliminary effects of Coffea arabica (CA) in experimentally-induced stroke in an animal model in the context of neuroprotection. The study was also designed to document the prophylactic use of CA in patients experiencing stroke symptoms.

Methods: A total of 25 male albino rats, 12 months of age, were purchased from the local market. They were acclimatized for seven days and divided into 5 groups. Each group consists of 5 subjects. Each subject was trained on a specific test for behavioral assessment. Behavioral modulation of each rat was performed using four tests, namely cylinder test, staircase test, forelimb flexion test, and pasta test. All the tests were performed as per standard criteria at the 4th, 8th, 12th, and 14th day of drug administration. The subjects were administered with caffeine (2.4 mg/kg) and CA (100 mg/kg and 200 mg/kg extract) doses for 14 days to assess the prophylactic use of CA. After 14 days of treatment, rats were subject to ischemic stroke induction using the middle cerebral artery ligation method. All four tests used for behavior modulation were applied at 24, 48, and 72 hours intervals of postsurgery. The subjects were further sacrificed for histopathological investigations. Statistical analysis was performed with SPSS (V. 22) software using one-way ANOVA.

Results: Our findings suggest that treatment with CA, 100 mg/kg and 200 mg/kg orally, decreases the infarct volume. However, there are not many considerable differences that were found in both doses. Histopathological investigations revealed characteristic structural changes occurring in both gray and white brain regions, depending on the severity, location, and duration of the ischemic stroke.

Conclusion: CA is a harmless and active mediator in the dissolution of blood clots and the recovery of stroke in rats. It is an agent that has been found to be efficient for brain activity with few side effects and behavioral modifications.
An annotated checklist of the macrofungi (Ascomycota, Basidiomycota, and Glomeromycota) from Quintana Roo, Mexico
Authors: de la Fuente Javier Isaac , García-Jiménez Jesús , López Caribell Yuridia , Oros-Ortega Iván , Vela-Hernández Reyna Yazuly , Guevara-Guerrero Gonzalo , Garza Ocañas Fortunato , Chay Casanova Jesús Antonio , Ibarra Garibay León Esteban , Bandala Victor Manuel
Published: May 2020

According to records available in the literature, 380 species of fungi and fungus-like organisms are known for the state of Quintana Roo on the Yucatán Peninsula of Mexico. We carried out mycological surveys in this state, following the classical methods for collecting, describing, and curating macrofungi. As a result of our research, 21 species are recorded for the first time for Quintana Roo, and Amanita arenicola O. K. Milller & Lodge, Inocybe xerophytica Pegler, and Russula cremeolilacina Pegler are recorded for the first time for Mexico. The distinctive characteristics and photographs are provided for the new records. As a result of this study, 401 species of macrofungi are known for Quintana Roo.
Transfusión de plasma convaleciente de pacientes con COVID-19: Propuesta de la Sociedad Peruana de Inmunología y la Asociación Médica Peruana de Patología Clínica para su implementación en nuestro país
Authors: Galvan Cesar Alberto , Toribio-Dionicio Crhistian Gabriel , Álvarez-Ángeles Marco Antonio , Alama-Bazán Óscar Daniel , Sánchez-Ramírez Luis Alberto Antonio
Published: May 2020

Actualmente no existe vacuna disponible ni medicación específica contra la enfermedad de Coronavirus 2019 (COVID-19). El tratamiento se basa fundamentalmente en medidas de soporte. En las últimas semanas, en varias partes del mundo, incluido nuestro país, se han dado flexibilidades para que la comunidad científica conduzca ensayos clínicos con terapias de potencial utilidad, como es el caso de la transfusión de plasma convaleciente (TPC) de pacientes con COVID-19. Se realizó una búsqueda en MEDLINE de estudios sobre plasma convaleciente y COVID-19, SARS o MERS. Los estudios sobre la eficacia clínica de este tratamiento en enfermedades causadas por otros coronavirus (SARS-CoV y MERS-CoV) evidenciaron mejoría clínica, aumento de anticuerpos neutralizantes, disminución de mortalidad y ausencia de eventos adversos durante y después del tratamiento. En el caso del COVID-19, se encontraron 6 estudios del uso de este tratamiento en pacientes con COVID-19 severo (en ventilación mecánica y con síndrome de distrés respiratorio agudo). Aunque existen limitaciones en la metodología, número de pacientes y protocolos para el análisis del plasma convaleciente de los donantes, los pacientes que recibieron TPC evidenciaron mejoría clínica, mejoría de patrones ventilatorios, resolución de lesiones pulmonares, disminución de mortalidad, mejoría de parámetros laboratoriales, aumento de anticuerpos neutralizantes, disminución de carga viral y ausencia de eventos adversos. Presentamos además un protocolo de realización de TPC y propuestas para su implementación y uso en nuestro país.
Patient characteristics, therapy service delivery and patient outcomes following pyrocarbon proximal interphalangeal joint arthroplasty
Authors: Hendricks Nureesah , Nadasan Thayananthee , Olagbegi Oladapo Michael , Chemane Nomzamo
Published: May 2020

BACKGROUND/AIM: Studies on likely sociodemographic and pre-surgical determinants of hand function and satisfaction following pyrocarbon proximal interphalangeal joint arthroplasty (PPIJA) are scarce. The primary aim of this study was to explore the association between pre-surgical sociodemographic and clinical characteristics and post-surgical hand function and satisfaction of patients who underwent PPIJA. A secondary aim was to evaluate the effects of the procedure on pain and active range of movement (AROM) using retrospective data and on-site follow-up assessment. METHODS: A panel survey of 48 patients (male = 13; female = 35) with median age of 64 years, who had PPIJA between 2001 and 2012, with a total of6l arthroplasties, was conducted. During follow-up, participants' pain and satisfaction, AROM, and hand disability were assessed using the Pain and Satisfaction Questionnaire (PSQ), goniometer, and the Disability of the Shoulder, Hand and Elbow (DASH) Questionnaire respectively. RESULTS: The main reason for surgery amongst participants was joint stiffness (68%) while 33.3% of the participants had a repeat surgery. Participants' median satisfaction and DASH scores at final assessment were 3 and 22.55 respectively. Patients who underwent arthroplasty once had significantly higher median PSQ scores (p = 0.011) than those who had their surgery repeated. Pain significantly reduced (p < 0.001) while AROM significantly increased (p = 0.001) from pre-operative assessment to final follow-up assessment CONCLUSIONS: Pyrocarbon arthroplasty improved treatment outcomes regarding pain and joint motion; post-operative satisfaction may be associated with patients having a repeat surgery. Keywords: Pyrolytic carbon, proximal interphalangeal joint replacement, arthritis, treatment outcome
Chromatin Endogenous Cleavage and high-throughput sequencing (ChEC-seq) inS. cerevisiae v1
Authors: Zentner Gabe
Published: May 2020

Genome-wide mapping of protein-DNA interactions is critical for understanding gene regulation, chromatin remodeling, and other chromatin-resident processes. Formaldehyde crosslinking followed by chromatin immunoprecipitation and high-throughput sequencing (X-ChIP-seq) has been used to gain many valuable insights into genome biology. However, X-ChIP-seq has notable limitations linked to crosslinking and sonication. Native ChIP avoids these drawbacks by omitting crosslinking, but often results in poor recovery of chromatin-bound proteins. In addition, all ChIP-based methods are subject to antibody quality considerations. Enzymatic methods for mapping protein-DNA interactions, which involve fusion of a protein of interest to a DNA-modifying enzyme, have also been used to map protein-DNA interactions. We recently combined one such method, chromatin endogenous cleavage (ChEC), with high-throughput sequencing as ChEC-seq. ChEC-seq relies on fusion of a chromatin-associated protein of interest to micrococcal nuclease (MNase) to generate targeted DNA cleavage in the presence of calcium in living cells. ChEC-seq is not based on immunoprecipitation and so circumvents potential concerns with crosslinking, sonication, chromatin solubilization, and antibody quality while providing high resolution mapping with minimal background signal. We envision that ChEC-seq will be a powerful counterpart to ChIP, providing an independent means by which to both validate ChIP-seq findings and discover new insights into genomic regulation.
50 anos da I Feira Nacional de Ciências (1969) no Brasil
Authors: Magalhães Danilo Castro , Massarani Luisa , Rocha Jessica Norberto
Published: May 2020

Os 50 anos da I Feira Nacional de Ciência no Brasil, vídeo produzido pelo Instituto Nacional de Comunicação Pública da Ciência e Tecnologia

Em 2019, comemoramos os 50 anos da primeira Feira Nacional de Ciências no Brasil. Realizada no Pavilhão de São Cristóvão, no Rio de Janeiro, em setembro de 1969, o evento contou com a apresentação de trabalhos de alunos oriundos de quase todos os estados brasileiros, alta frequência de público visitante e ampla cobertura jornalística. O Ministério da Educação e a Secretaria de Ciência e Tecnologia do então Estado da Guanabara coordenaram o evento, com parcerias e patrocínios públicos e privados. Neste artigo, realizamos uma análise de 174 matérias de jornais que noticiaram a Feira na época, identificadas a partir de uma busca na Hemeroteca Digital da Biblioteca Nacional e em acervos digitais de jornais de grande circulação. Com base nesse material, trazemos informações sobre a organização, o patrocínio, os antecedentes e a realização da Feira, bem como as principais e mais recorrentes temáticas abordadas na cobertura jornalística.
A INTERFACE ENTRE DESIGN E SAÚDE: UMA REVISÃO BIBLIOGRÁFICA
Authors: Dastre Manzanares Raquel , Bragança Lana Sebastiana Luiza
Published: May 2020

Este artigo tem o objetivo de realizar uma revisão bibliográfica quantitativa em bases de dados científicos – Portal de Periódicos da CAPES, Scielo e Portal Regional da Biblioteca Virtual em Saúde (BVS) – no período de cinco anos (2011-2016). Foram coletados os artigos científicos nacionais relacionados à disciplina de design em interface com a área de saúde e suas referências bibliográficas analisadas. Os resultados apontam para uma disparidade quantitativa entre a produção científica brasileira e a internacional nesse eixo temático. O fato do tema “saúde” não representar uma linha principal da pesquisa em design destaca o potencial de um campo emergente e promissor.
A TRAJETÓRIA FEMININA E AS DESIGUALDADES SEXUAIS: UMA REFLEXÃO CRÍTICA ACERCA DO TRABALHO
Authors: Santos Juliana de Jesus , Ferreira Maria da Luz Alves
Published: May 2020

Este artigo traça a realidade entre homens e mulheres nas relações de trabalho e suas implicações na trajetória feminina considerando a histórica desigualdade de gênero. Para ilustrar essa realidade, partiu-se de uma pesquisa bibliográfica onde alguns dados auxiliarão no entendimento do referido problema. Assim, o objetivo dessa análise é salientar a existência do paradoxo do trabalho entre homens e mulheres. Nessa situação, a análise destaca também a conjuntura vivida pela mulher no acesso ao trabalho, além dos percalços e avanços conquistados ao longo de vários anos de luta e reivindicações por dignidade e melhores condições de vida. Essa análise nos permitiu pressupor que o estigma da inferioridade ainda prevalece no cotidiano feminino embora a mulher tenha alcançado alguns avanços marginais.
JUVENTUDES E TRABALHOS: TRAJETÓRIAS DE EGRESSOS DO PROGRAMA JOVEM APRENDIZ
Authors: ROSA Debora Diana da , Coutinho Maria Chalfin
Published: May 2020

Resumo

O presente artigo apresenta resultados de uma pesquisa de mestrado que buscou compreender os sentidos sobre suas trajetórias construídos por egressos do Programa Jovem Aprendiz. Ancorada na psicologia histórico-cultural, a pesquisa fez uso do método da história oral para conhecer as histórias laborais de jovens egressos do referido programa. Foram entrevistados quatro jovens com idades entre 16 e 25 anos. A fotografia como memória e uma produção textual sobre projetos de futuro também foram usadas como procedimentos auxiliares para levantamento de informações. O processo de análise se deu a partir do referencial dos núcleos de significação e abarcaram questões como: Trajetória Laboral e Educacional, Experiências como Jovem Aprendiz, Ser Egresso e Projetos. Estes núcleos evidenciam a necessidade de pensar ações relacionadas à qualificação, inserção e permanência dos jovens no campo laboral e expressam a multiplicidade e pluralidade das vivências da condição juvenil, educação e trabalho.

Palavras-chaves: Jovens, Educação, Trabalho
RELAÇÕES INTERNACIONAIS E O EUROCENTRISMO: A CRÍTICA DO PENSAMENTO DECOLONIAL E O BEM VIVER COMO ALTERNATIVA
Authors: Madruga Leonardo Agrello
Published: May 2020

Este artigo tem como objetivo propor um breve debate entre a crítica pós-colonial no âmbito das Relações Internacionais e as vertentes de concepções surgidas na América Latina – o pensamento decolonial e os entendimentos do Bem Viver –, em busca de chamar atenção a outras possiblidades de informar o campo, que não apenas a preponderância em fatos e concepções surgidas do eurocentrismo. Em primeiro momento, faz-se uma abordagem breve sobre o pós-colonialismo nas Relações Internacionais, tratando de se apontar as críticas ao caráter eurocêntrico da área. Adiante, segue-se com a análise do pensamento decolonial latino-americano, a fim de discutir como esses teóricos apresentam a crítica no pensamento social, em maior escopo. A discussão se encerra com a apresentação das ideias do Bem Viver, conceito baseado, essencialmente, nas concepções dos povos andinos e amazônicos. Trata-se de demonstrar como, por meios que se iniciam no processo de colonização da América, a história que informa o campo das Relações Internacionais é centrada na visão europeia-ocidental, ocultando partes relevantes do passado e, com isso, outras visões de mundo, motivos pelos quais se encerra a discussão com a abordagem do Bem Viver como alternativa.
VIOLÊNCIA NA INTIMIDADE DE JOVENS – REFLEXÕES A LUZ DA LITERATURA
Authors: De Souza Daniel Cerdeira , Da Silva Iolete Ribeiro , Honorato Eduardo Jorge San'Tana
Published: May 2020

Este estudo tratou-se de um recorte teórico de uma dissertação de Mestrado em Psicologia da Universidade Federal do Amazonas e discute o fenômeno da violência na intimidade de jovens com idade entre 15-29 anos aluz de uma revisão de literatura de cunho narrativo em abordagem qualitativa. Analisamos livros, artigos e dissertações que pudessem contribuir com o enriquecimento teórico reflexivo da proposta. Os achados foram analisados a partir de uma perspectiva descritiva e crítica. Os resultados apontam para a violência como um problema de ordem social que atinge o espaço privado das relações íntimas, sendo a violência na intimidade de jovens a expressão da violência de gênero, onde a gravidade da violência tende a aumentar com o passar do tempo na relação. Jovens em situação de violência na intimidade quase nunca procuram ajuda profissional para lidar com essa questão e não saem do relacionamento e quando conseguem sair, acabam por se envolver com relacionamentos abusivos crônicos.
Safflower: importance, use and economical exploitation
Authors: Menegaes Janine Farias , Nunes Ubirajara Russi
Published: May 2020

The safflower is an ornamental, medicinal, oleaginous, annual and herbaceous plant, and it is cultivated in more than 60 countries. In Brazil, it presents great edaphoclimatic adaptations for the different regions of the country. With wide economic exploitation, it encompasses: the floricultural sector with the floral stems in a fresh and dry form; the animal feeding with grazing, silage and seed cake; the food, pharmaceutical and oil industries with the use of oil according to its refine for the emulsification in bakery, elaboration of medicine and biodiesel; besides the use of coloring that comes from the dried petals in culinary, cosmetics and in fabrics, among other aptitudes of use. Thus, the present work had as objective to perform a national and international review of literature about the importance, use and economic exploitation of safflower.
Quantification of litter and organic carbon in fragment of cerrado sensu stricto in Tocantins
Authors: Santana Thiêssa Tamilla de Carvalho , Aguiar Bruno Aurélio Campos , Teixeira Lorena Gama , Gama Rafaela Da Costa , Santos André Ferreira dos , Souza Priscila Bezerra de
Published: May 2020

This study aimed to quantify the accumulated litter of a fragment of cerrado sensu stricto, besides estimating the organic carbon stored from the organic matter. We conducted the study in a sensu stricto cerrado fragment in the municipality of Formoso do Araguaia, State of Tocantins, where samples of litter were collected in six random plots of 10 m x 50 m (500m2) using a 0.5 m x 0.5 m iron template by throwing it into the ground to collect the material. We separated the collected litter into two fractions, dry and green litter. Those collected were conditioned in paper containers for oven drying at 70°C for 48 h. It was weighed 1.5 g of the biomass ground in porcelain crucibles, which were dried in an oven at 105°C for 24h, then in the muffle at 550°C for 3h, at the Madeira Laboratory from the Federal University of Tocantins. For the litter, it was quantified 4.36 t ha-1 (value below the ones found in the literature), 69.08% of organic matter for the dry material, 95.42% of organic matter for the green material, 0.546 t ha-1 of organic carbon in the dry material, 0.769 t ha-1 of organic carbon in the green material.
Feed intake of sheep receiving ration with addition of passion fruit peel meal
Authors: Aguiar Sílvia Cristina de , Geron Luiz Juliano Valério , Nunes Gabriel Maciel , Garcia Jocilaine , De Carvalho Joilma Toniolo Honório , Silva Ana Paula da
Published: May 2020

Part of the costs of animal production is food, and consequently, several alternatives have been examined for use as co-products, such as passion fruit peel meal (PFP). The objective of this study was to evaluate the dry matter (DMI), nutrient intake, feeding behavior, and physiological parameters of sheep receiving PFP in their ration. Four mixed breed sheep were with a mean body weight of 38.4 kg and distributed in a 4 × 4 Latin square. The experimental diet was formulated with 50% corn silage and 50% concentrate (dry matter [DM] basis), to contain 16% crude protein (CP) and 70% total digestible nutrients, with the addition of PFM to the feed at 0%, 1.6%, 5.8%, and 8.9% based on DMI. The addition of PFP did not affect (P > 0.05) DMI and nutrients, with an average DMI of 838.51 g d-1, 2.21% of body weight, and 54.59 g kg-0.75. The addition of PFP to the ration did not change the feed intake of DM and nutrients. The PFP did not change the ingestive behavior of sheep during the dawn, morning, and afternoon. However, an increase in the intake time and leisure time was observed during the night. PFP did not influence any of the physiological variables; however, the high temperature during the execution of the experiment caused animal discomfort both in the morning and in the afternoon.
Action of formulated rosemary against Meloidogyne incognita in soybean
Authors: Cazzo Victor Natan , Stangarlin José Renato , Mattei Danielle , Kuhn Odair José , Cavilia Junior Fortunato , Lorenzetti Eloisa
Published: May 2020

The damage caused by nematodes has been severe in many crops in Brazil, causing a reduction in agricultural yield. The objective of this work was to evaluate the efficiency of a formulation, elaborated with extract of the rosemary (Rosmarinus officinalis) and cassava bark, on the control of nematode Meloidogyne incognita in soybean. The doses evaluated were 0.0; 25; 50; 100; 150 and 200 mL of the dried formulation per pot of 3 L containing soybean plants. The experiment was conducted in a greenhouse, in a randomized block design, with five replications. We evaluated dried and fresh masses of aerial part, fresh mass of root, and the number of egg mass, galls and second stage juveniles (J2) and eggs in root and soil. The soybean plants showed growth reduction resulting from the interaction of the formulation, maybe due a phytotoxic effect from cassava bark. There was allelopathic effect in soybean plants treated with formulated made with cassava bark + aqueous extract of rosemary, evidenced by inhibition in plant development according to the increase in doses of the formulated. Moreover, the formulated was not efficient in the control and or reduction of M. incognita.
Winter cold deficit in low subtropical climate region and blackberry varieties phenology
Authors: Villa Fabíola , Silva Daniel Fernandes da , Stumm Diego Ricardo , Rosa Danimar Dalla
Published: May 2020

In the western region of Paraná, the subtropical climate prevails, with little accumulation of hours of cold in the winter, making the fruit species not go into complete dormancy. Given the above, the objective of this study was to evaluate the effect of chemical defoliant on blackberry varieties, aiming at their complete defoliation and induction of numbness. The work was conducted at Unioeste, Marechal Cândido Rondon Campus, in a four-year-old orchard of plants, spaced 3 x 0.5 m, simple spreader with two wire strands at a height of 120 cm from the ground, and 20 plants per grow crops. The experimental design used was randomized blocks, where two treatments related to the application of defoliant (with and without) on 7 cvs were evaluated blackberry, containing three replicates and three plants in each cultivar. Was used as a defoliant lime sulfur applied in August pruning then held 25 days after application. Regarding fruit quality, Caigangue and Tupy varieties showed the highest SS/AT ratio, indicating the best flavor of their fruits for fresh consumption. The cultivars Brazos and Guarani showed lower SS/AT ratio, indicating more acidic fruits. Higher levels of ascorbic acid were found in fruits of cvs. Guarani and Cherokee. The application of defoliant reduced the total number of blackberry fruits of the cultivars Comanche and Guarani. The use of defoliant did not interfere in the physicochemical characteristics of the blackberry fruits of the evaluated cultivars.
Bacterial pathogens in wound infection and their antimicrobial susceptibility pattern in a medical college hospital, in Dhaka, Bangladesh
Authors: Afroz Sadia , Sarkar Dipika , Khatun Khadeza , Khan Tarek Mahbub , Paul Shikha
Published: May 2020

Background: Wound infection is a major health problem that results in prolong hospital stay, increased treatment cost and are responsible for significant mortality and morbidity worldwide. The aim of the present study was to isolate and identify the bacterial pathogens causing wound infection and to determine their antimicrobial susceptibility profile.Methods: This cross sectional study was conducted at the Department of Microbiology, Sir Salimullah Medical College, Dhaka from January 2016 to December 2016. Wound swab samples were collected and inoculated into appropriate media. The bacterial pathogens were identified by using standard microbiological methods. Antimicrobial susceptibility test were performed using disc diffusion technique following Kirby-Bauer method.Results: Out of 239 wound swab samples analyzed 173 (72.4%) were culture positive. Majority (35.3%) of culture positive cases were in age group 16-30 years and 60.1% were male. Staphylococcus aureus (36.9%) was the predominant isolate, followed by Escherichia coli (35.8%), Pseudomonas spp. (17.3%) and Proteus spp. (5.8%). Bacterial isolates were highly resistant Amoxicillin (89-100%), Cephalosporin (60-100%), Ciprofloxacin (53-71%), while they were least resistant to Imipenem (0-14%) and Amikacin (17-30%).  Conclusions: In the present study most of the isolates showed high rate of resistance to commonly used antibiotics. Therefore regular monitoring and rational use of antibiotic should be practiced.
Sutures versus staplers for skin closure in laparotomy patients: a prospective study
Authors: Sharma Gopal , Prasher Nivedita
Published: May 2020

Background: Every surgeon wants cosmetically acceptable scars along with optimal healing. Good tissue union and cosmetically acceptable scars are vital for ideal surgical practice. A basic need for skin closure is a good approximation. Apart from cosmetically good scars, it is also necessary that the skin closure technique should be technically easy, speedy, economical and acceptable.Methods: The study was conducted on 100 patients on whom elective abdominal surgeries were performed. Patients were divided into two groups with 50 patients in each group after matching the parameters like age, co morbid conditions, using simple random sampling technique. All operations were performed by one consultant. In group A, Skin was approximated with vertical mattress sutures while in group B, staplers were used to close the wound.Results: The age of the patients varied from 16 to 85 years. The average time taken for skin closure for suture group (A) was found to be 300sec (±20.78) and for stapler group was found to be 120sec (±16.50) respectively. Wound infection was found in 10 patients (20%). In stapler group 4 (8%) and in suture group 6 Patients (12%) had post-operative wound infection.Conclusions: Cosmesis is essential and necessary in modern surgical practice. It also reflects surgical expertise.
Efficacy of homologous fibrin sealant in the surgery for pneumothorax
Authors: Saricam Murat
Published: May 2020

Background: Prolonged air leakage following pneumothorax surgery is a significant issue causing increased hospital stay and morbidity. This study aimed to investigate the cost and efficacy of homologous fibrin sealant in preventing the air leakages.Methods: Among the patients who had undergone bullectomy and subtotal parietal pleurectomy for recurrent primary spontaneous pneumothorax via transaxillary mini thoracotomy between 2010 and 2018, two groups each including 35 cases were conducted as to whether fibrin sealent had been applied. These two patient groups were compared in terms of age, gender, duration of air leakage and cost.Results: Mean age of whole group of patients including 59 males and 11 females was 21.5 years. Mean values of air leakage duration and cost of hospital stay was calculated as 1.94 days and 2777 TL for sealant applied group and 2.97 days and 1200 TL for sealent unapplied group, respectively. The patient groups did not indicate a statistically difference in terms of age and gender whereas duration of air leakage was shorter but cost was higher in the group for whom fibrin sealant had been administered (p<0.001). None of the patients developed mortality but recurrence was present in 4 (5.7%) patients.Conclusions: Although homologous fibrin sealant applied in pneumothorax surgery results in cost increase, it contributes to surgical outcomes by preventing possible additional complications in consideration of shortened duration of air leakage.
Impact of transition in socio-economic status on sonographically detected non-alcoholic fatty liver disease: a study based on modified Kuppuswamy classification in an urban population
Authors: Jain Abhinav , Manjavkar Smita , Jafri Humaid Ali , Anand S. S.
Published: May 2020

Background: The transition in the socioeconomic status is associated with physical and mental challenges. The lifestyle modification is one of the key implicatory for this change. Non-alcoholic fatty liver is an ever-growing health concern. This study evaluates the socioeconomic categories based on Kuppuswamy classification and other correlated in sonographically detected Non-alcoholic fatty liver disease (NAFLD).Methods: This cross-sectional study was conducted on 300 sonographically detected NAFLD patients in the age group of 18-60 years. Detailed history, including the demographic profile, socio-economic status (Modified Kuppuswamy scale was followed for calculating the Socio-economic status of the family), occupation, dietary habits (vegetarian or non-vegetarian, exposure to junk food), drinking water supply, etc. Detailed assessment of the morphological parameters including the anthropometric measurements, height, BMI was also assessed.Results: The study finds 62 (41%) females and 88 (59%) males with NAFLD and 51-60 age group with maximum prevalence. Diabetes (63%), Soft drink consumption and obesity are important risk factors. New observation of our study is that amongst various Kuppuswami sociodemographic scales, the maximum patients belonging to Upper middle class and upper lower class presented with fatty liver.Conclusions: There is higher prevalence of NAFLD amongst males, diabetics, obese, soft drinks, tea and coffee consumers. Authors also find a unique correlate based on socio-demographic class of Kuppuswami scale. People belonging to upper middle class and upper lower class suffer from NAFLD more commonly than other sociodemographic classes.
Multi detector computed tomography imaging in penetrating injuries
Authors: Sachdev Namrita , Singh Yashvant , . Sana , Mehta Dipshi
Published: May 2020

Background: Penetrating injuries forms an important component of surgical emergencies. Penetrating trauma typically involves the violation of the body by a gunshot wound (GSW) or stab wound. Emergency laparotomy is the accepted management in patients with a penetrating injury who are not hemodynamically stable. However, selective non-operative management has been shown to decrease the rate of unnecessary surgery. Plain radiographs and FAST are useful for initial assessment of these patients. Multi detector CT is an indispensable tool in the evaluation of patients who are stable and are candidates for conservative treatment.Methods: A cross sectional observational study was carried out on 40 patients admitted with penetrating injury to the trauma centre of PGIMER and Dr RML hospital. Of the 40 patients, 14 were taken for emergency laparotomy. In 26 cases, MDCT was done to evaluate for solid organ, hollow organ and vascular injuries.Results: The mean age of patients was 38 years, with male to female ratio of 5:1. Stab injury was the commonest type of injury, followed by gunshot wounds. Liver was the commonest solid organ involved, followed by kidneys and spleen. Other organs involved were diaphragm, small bowel and colon.Conclusions: Penetrating injuries can be life-threatening, making prompt diagnosis essential. Recognizing key abnormalities on MDCT ensures patients are triaged appropriately. Multi-detector CT is thus widely used to evaluate penetrating injuries in stable patients.
Using positron emission tomography - computed tomography imaging to distinguish of metastatic disease from second primary lung tumours in patients with non-small cell lung cancer
Authors: Xuan Ngo Minh , Huy Huynh Quang
Published: May 2020

Background: In NSCLC patients with multiple lesions, the differentiation between metastases and second primary tumours has significant therapeutic and prognostic implications. The aim of this retrospective study was to investigate the potential of 18F-FDG PET to discriminate metastatic disease from second primary lung tumours.Methods: Of 318 NSCLC patients between November 2015 and October 2018 at Bach Mai hospital, patients with a synchronous second primary lung cancer were selected. Patients with metastatic disease involving the lungs served as the control group. Maximum standardized uptake values (SUVs) measured with 18F-FDG PET were determined for two tumours in each patient. The SUVmax was determined and compared between the second primary group and metastatic disease group. Receiver-operating characteristic (ROC) curve analysis was performed to determine the sensitivity and specificity of the SUVmax for an optimal cut-off value.Results: A total of 81 NSCLC patients (44 metastatic disease, 37 second primary cancer) were included for analysis. The SUVmax was significantly higher in patients with second primary cancer than in those with metastatic disease (7.53±4.33 vs 4.35±2.58, respectively, p<0.001). The area under the ROC curve was 0.81 and the odds ratio for the optimal cut-off was 7.52.Conclusions: SUVmax from 18F-FDG PET images can be helpful in differentiating metastatic disease from second primary tumours in patients with synchronous pulmonary lesions. Further studies are warranted to confirm the consistency of these results.
Comparison of ropivacaine alone or with dexamethasone as an adjuvant for reducing pain during positioning for neuraxial blockade with ultrasound-guided fascia iliaca compartment block
Authors: Dubey Suvir , Ambi Uday , Taank Priya , Singh Shalendra , Marwah Vikas
Published: May 2020

Background: Comparison of ropivacaine alone or with dexamethasone as an adjuvant for reducing pain during positioning for neuraxial blockade with ultrasound-guided fascia iliaca compartment block.Methods: In this double-blinded study, a total of 60 patients between 18 to 80 years of age, undergoing surgery for hip fracture were enrolled. Patients in Group A received 40 ml of 0.25% ropivacaine +2 ml saline and patients in Group B received 40 ml of 0.25% ropivacaine +8 mg dexamethasone. USG guided FICB and postoperative monitoring was done by the chief investigator who was unaware of group allotted and drug administered.Results: There is no significant difference in the heart rate between the two groups after 30 min of the block. The variation of systolic blood pressure of both the group for the first 30 min after giving FICB block was not significant (p>0.05). The absolute value of diastolic blood pressure (DBP) was significantly lower in Group B compared to group A just before the block, a variation of DBP with time was not significant. There was a gradual improvement of pain score from mean 6.7 in Group A and 6.6 in Group B at 0 min to score of 2 at the end of 30 min in both the group. This improvement was achieved earlier in Group B compared to Group A, although the difference was not significant (p>0.05). Vital parameters like HR, SBP, DBP, SpO2 values were similar in both the groups. No patients in either group required any interventions both pre-operatively and pos-operatively. Time of rescue analgesia was noted with the VAS score was significantly more in Group B (p≤0.004). The incidence of hematoma, accidental intravascular injection, convulsion, and paresthesia were nil in both groups.Conclusions: Although both the groups had comfortable and pain-free positioning for administering spinal anaesthesia before surgery. USG guided FICB is easy to perform block and give excellent analgesia for positioning and mobilization of hip fracture patients pre and post-operatively both, and dexamethasone as an adjuvant to 0.25%ropivavaine prolong its local anesthetic effect significantly.
Extranodal rosai dorfman disease: a case report of single soft tissue cystic lesion
Authors: Agrawal Ruchi , Saha Anurag , Baro Bhanita
Published: May 2020

Rosai-Dorfman disease (RDD) is an uncommon benign histiocytic disorder. It is found worldwide and slightly more common among in men (1.4:1), affecting individuals are with an average age of 20.6 years. Extranodal tissue involvement is documented in 25-43% of RDD patients. Here authors are discussing a case of 16-year-old male patient with the complaint of a single swelling over left forearm since one month with no lymphadenopathy. Radiology showed anechoic collection within the muscle and superficial to it. On microscopy, smear showed diffusely distributed histiocytes with cytoplasm showing intact lymphocytes with halo surrounding it (emperipolesis), without atypia. Based on cytomorphology diagnosis of Extranodal Rosai Dorfman Disease was made.
Transcutaneous pacing: a life saviour
Authors: Mishra Roly , Thakkar Parna , Mehta Hemant
Published: May 2020

Doukky Rand colleagues acknowledged that Transcutaneous Cardiac Pacing is a temporary method of pacing which may be indicated in patients with symptoms of severe or hemodynamically unstable bradyarrhythmias. It is found to be extremely helpful in patients with reversible or transient conditions, such as digoxin toxicity and in atrioventricular block in the case of inferior wall myocardial infarction, or when transvenous pacing is not available or there are high chances of complications. Widened QRS complex indicates successful attempt followed by a distinct ST segment and broad T wave. The hemodynamic response to pacing is to be confirmed by the assessment of patient’s arterial pulse waveforms. Trancutaneous pacing is a boon to manage and treat intra op dangerous bradycardia.
Production of annatto seedlings on substrates based on decomposed babassu stem
Authors: Silva Taciella Fernandes , Do Lago Lopes Klayton Antonio , Da Silva Igor Alves , Dos Santos Janaiane Ferreira , Freitas de Andrade Hosana Aguiar , Salustriano da Silva-Matos Raissa Rachel
Published: May 2020

Annatto (Bixa orellana L.) is an important species for several sectors, industrial, food, landscape, among others, which uses parts of the plant or the entire plant. It is an underresearched species, although it has significant economic value for small-scale farmers. The substrate is one of the sources that directly influence the production of a perennial species that need to present good characteristics to ensure a quality seedling. Therefore, the aim was to evaluate the babassu stem decomposed in the formulation of substrates for the production of annatto seedlings. The study was carried out a shading screen with 75% permeability to light. The experiments were conducted in a completely randomized design, with four replications. The treatments consisted of substrates based on babassu stem decomposed (BSD), in the following proportions: S0: 100% soil; S20: 20% BDS + 80% soil; S40: 40% BDS + 60% soil; S60: 60% BDS + 40% soil; S80: 80% BDS + 20% soil; S100: 100% BDS. At 90 days after sowing (DAS), an evaluation of seedling growth variables was performed. A difference was found between the procedures for all the variables analyzed. There was a difference between treatments for all variables analyzed. The substrate constituted of 60% babassu stem decomposed is the most appropriate for the production of taller annatto seedlings.
A study in respect of transfusion related complications in hemophiliacs with special reference to developing factor inhibitors
Authors: Sharma Praveen
Published: May 2020

Prophylaxis and treatment with factor replacement have greatly improved the quality of care for patients with hemophilia.However,development of factor inhibitors is the most serious and challenging complication of therapy. Other complications are viral infections like Hepatitis B, Hepatitis C, and HIV, and the other infective diseases, which can be transmitted by the blood and blood products transfusion.

The aims of this study is to study the complications in the hemophiliacs who have been treated prophylactically or ‘on demand’ with fresh frozen plasma, cryoprecipitate and concentrated products of FVIII and

During the study period, all patients (100) with Hemophilia attending Gandhi Medical College are taken under consideration. The presence of an inhibitor was determined by a simple mixing experiment using the test plasma and normal pooled plasma and 3 generation enzyme linked immunosorbent assay (ELISA) method& anti-HBsAg.statistical

The inhibitor study showed that in 7%(3.2-13.98 at 95% confidence interval) patients, APTT was not corrected after mixing patients plasma with pooled normal plasma (PNP) and applying the test immediately and after one hour of incubation. serological tests showed antibodies for HCV were positive in 4% of cases (1.1%-9.93% at 95% CI), whereas antibodies against HBsAg was positive in 1% of cases(0.3%-5.45% at 95% CI), which was less then anti HCV. Transfusion associated complications were higher in severe form of Haemophilia as compared to moderate and mild form of Hemophilia.

Developing antibodies to infused factor concentrates (inhibitors) remains a major source of morbidity and mortality in the treatment of patients with hemophilia. Novel treatment approaches for these patients are in developmental stage, which include therapeutic agents that mimic factor VIII or augment thrombin production by bypassing the inhibitors, as well as agents that act by inhibiting the natural anticoagulants.
Pyloric gland adenoma of gallbladder with squamoid morules in pediatric age
Authors: Choraria Amit , Gupta Ashutosh , Tiwari Shantanu , Tikeykar Vishakha , Chaudhary Vivek
Published: May 2020

7 year old symptomatic girl was diagnosed with gall bladder polyp on ultrasonography and MRCP (magnetic resonance cholangiopancreatography). Laparoscopic cholecystectomy was performed and patient was discharged on 2 post operative day without any complications. Histopathology and Immunohistochemistry revealed pyloric gland adenoma with squamoid morules.

Gall bladder polyps are seen in 5% of the adult population around the world but rarely seen in children. Polyps have the potential to convert into malignancy and hence early diagnosis and differentiation is necessary.

Most of the patients with gall bladder adenomas are adult females. Majority of the adenomas (91%) are single. Squamoid morules was found in 28% and columnar oxyphil cells in 2% of these adenomas. High-grade dysplasia/carcinoma in situ was seen in 27% of them and low-grade dysplasia in 15%. However, only 1% invasive adenocarcinomas were diagnosed in pyloric gland adenomas, both of which were intestinal type. For symptomatic patients who have pain and dyspepsia, cholecystectomy is the recommended treatment. For asymptomatic or incidentally detected patients, the indications for cholecystectomy should be age more than 50 years, solitary polyp greater than 10mm in largest dimension, accompanying gall stones and increase in size on serial sonographies.

As per our knowledge the case we report is the first case of gallbladder pyloric adenoma with squamoid morules in pediatric age group. Treatment with simple cholecystectomy is recommended in view of literature suggesting pre malignant lesion.
Comentarios acerca del artículo: “Historias de vida familiar en madres adolescentes: estudio cualitativo realizado en el Hospital Engativá ESE en Bogotá, Colombia”
Authors: Rodríguez-Soto Karen , Reyes-Aranibar Sara , Uribe-Chincha Tula , Torres-Slimming Paola
Published: May 2020

Carta al editor del 17 de noviembre de 2019 sobre el artículo “Historias de vida familiar en madres adolescentes: estudio cualitativo realizado en el Hospital Engativá ESE en Bogotá, Colombia” https://doi.org/10.18597/rcog.3162
La comunicación institucional de contenidos sanitarios mediante notas de prensa y su reflejo en la prensa diaria. El caso de Aragón = Institutional communication of health contents through press releases and its presence in the daily press. The case of Aragón
Authors: García-Latorre Florencio-Jesús , Aibar-Remón Carlos , Gobantes-Bilbao Maite
Published: May 2020

Resumen: Introducción: La publicación de notas de prensa es una práctica habitual de los gabinetes de comunicación de los Departamentos de Salud autonómicos mediante la que ofrecen información relevante para un mejor conocimiento y utilización del sistema, la difusión de sus actividades y la rendición de cuentas. Objetivo: Analizar las características de los comunicados de prensa emitidos por la Dirección de Comunicación del Gobierno de Aragón y verificar el grado en que los temas tratados en estas informaciones obtienen visibilidad en los medios impresos. Material y método: Revisión de las notas de prensa de contenido sanitario durante un año y comprobación de si los temas propuestos han sido llevados a las páginas de los dos periódicos de ámbito autonómico de la comunidad. Resultados: Se encontraron 190 notas de prensa, generalmente centradas en aspectos de la gestión sanitaria. Un 43% no obtuvieron reflejo en la prensa. Entre los dos medios estudiados se observa una concordancia moderada en cuanto a los temas publicados. Conclusiones: La comunicación institucional puede ser considerada un tipo de comunicación política, con unas características particulares, que es filtrada y contrapesada por los medios dentro de su labor de control de las instituciones públicas.Palabras clave: Salud, Comunicación institucional, Notas de prensa, PrensaAbstract: Introduction: The publication of press releases is a common practice of press offices of the Regional Departments of Health to offer relevant information for a better knowledge and use of the health system, to publicize their activities and for accountability purposes. Objective: To analyze some features of the press releases issued by the Directorate of Communication of the Government of Aragon and also verify the extent to which the subjects covered in these informations obtain visibility in the print media. Material and method: A review of the health-related press releases during one year was carried out; we also checked whether the proposed issues were brought to the pages of the two regional newspapers. Results: 190 press releases were found, mainly focused on aspects of health management. 43% of those reports were not mentioned in the newspapers. Between the two dailies studied, a moderate level of agreement in the selection of the subjects that were translated into news was observed. Conclusions: Institutional communication can be considered a type of political communication, with particular features, that is filtered and counterbalanced by the media, given that one of its tasks is the monitoring and control of the performance of public institutions.Keywords: Health, Institutional communication, Press releases, Press