bluuebunny/crossref_metadata_2025 huggingface dataset:
Identifying long non-coding RNAs involved in heat stress response during wheat pollen development
Authors: Babaei Saeid , Bhalla Prem L. , Singh Mohan B.
Published: February 2024

Introduction

Wheat is a staple food crop for over one-third of the global population. However, the stability of wheat productivity is threatened by heat waves associated with climate change. Heat stress at the reproductive stage can result in pollen sterility and failure of grain development.

Methods

This study used transcriptome data analysis to explore the specific expression of long non-coding RNAs (lncRNAs) in response to heat stress during pollen development in four wheat cultivars.

Results and discussion

We identified 11,054 lncRNA-producing loci, of which 5,482 lncRNAs showed differential expression in response to heat stress. Heat-responsive lncRNAs could target protein-coding genes in cis and trans and in lncRNA-miRNA-mRNA regulatory networks. Gene ontology analysis predicted that target protein-coding genes of lncRNAs regulate various biological processes such as hormonal responses, protein modification and folding, response to stress, and biosynthetic and metabolic processes. We also noted some paired lncRNA/protein-coding gene modules and some lncRNA-miRNA-mRNA regulatory modules shared in two or more wheat cultivars. These modules were related to regulating plant responses to heat stress, such as heat-shock proteins and transcription factors, and protein domains, such as MADS-box, Myc-type, and Alpha crystallin/Hsp20 domain.

Conclusion

Our results provide the basic knowledge and molecular resources for future functional studies investigating wheat reproductive development under heat stress.
Factors influencing self-efficacy for self-management among adult people with human immune deficiency virus on antiretroviral therapy in public hospitals of south-west Ethiopia
Authors: Abdisa MisganaTesgera , Gindaba Bekan Gudeta , Zerihun Ebisa
Published: February 2024

Introduction

Self-management is crucial for effective HIV management, and self-efficacy is a mechanism for achieving it, but there is limited evidence on variables that affect self-efficacy. This study aimed to identify factors influencing self-efficacy for self-management among adults on antiretroviral therapy in resource constraint settings.

Methods

A cross-sectional study was conducted among 422 adult people on antiretroviral therapy in southwest Ethiopia from March to April 2022. Face-to-face interviews were used to gather data using a structured questionnaire on the self-efficacy measure. The data were then imported into Epi Data version 4.2 and exported to SPSS version 26. Descriptive statistics, independent tests, one-way analysis of variance, Pearson correlation, and multivariate linear regression were used to analyze the data. The predictors with p-value of less than 0.05 were declared statistically significant.

Results

A total of 413 adults on antiretroviral therapy were interviewed with response rate of 97.9%. The total mean score of self-efficacy for self-management was 15.12 (±2.22) out of 24. Higher age, gender of the female, divorced, duration of diagnosis, and drug side effects were negatively predictors of low self-efficacy. Higher schooling, urban residence, better income, and the use of reminders positively influenced self-efficacy for self-management.

Conclusion

The study found low self-efficacy among adults on antiretroviral therapy and higher age, female gender, HIV duration, and presence of drug side effects were associated with lower self-efficacy, while higher schooling, better income, and use of reminder use were associated with higher self-efficacy for self-management. Further research is needed to determine the causal relationship between these variables and self-efficacy.
Diminished hedonic capacity in social activities as a mediator of the link between dysfunctional behavioral activation system and depressive symptoms
Authors: Kuan Chi-son , Liu Qian-yu , Xu Gang-min , Zhou Han-yu , Nie Jia-hui , Yan Chao
Published: February 2024

Background

Adolescence is a crucial period for the development of depression, and previous studies have suggested that the Behavioral Activation System (BAS) plays a significant role. However, little is known about the underlying mechanisms. This study aimed to explore the mediating role of anhedonia in the relationship between BAS and depressive symptoms among Chinese adolescents.

Method

A total of 1,023 high-school students aged 15–18 years participated in the study, with 916 continuing their participation three months later. All participants completed the Behavioral Inhibition System/Activation System (BIS/BAS) scale, Dimensional Anhedonia Rating Scale (DARS), Children’s Depression Inventory (CDI), and the State-Trait Anxiety Inventory (STAI-S/T). Pathway model analysis was performed to examine the concurrent and prospective mediating effects of anhedonia and the potential moderating effect of sex.

Result

Anhedonia in the domains of social activities, hobbies and sensory experiences significantly mediated the cross-sectional relationship between BAS and depressive level three months later. Furthermore, the beta-value of the mediating effect of social activities was significantly higher than that of the other domains of hedonic capacity cross-sectionally and longitudinally. However, sex showed no significant moderating effect.

Conclusion

Our findings underscore the importance of hedonic capacity, especially within the social domain, in the development of depressive symptoms. These findings contribute to the early diagnosis and prevention of depressive disorders.
Suicide prevention during disasters and public health emergencies: a systematic review
Authors: Reifels Lennart , Krysinska Karolina , Andriessen Karl
Published: February 2024

Background

Disasters and public health emergencies increasingly affect populations around the world, posing significant wide-ranging challenges for societies as well as for effective public health and suicide prevention. Intervention research is essential to inform evidence-based responses. Yet, despite evident public concern and growing research interest in heightened suicide risks and impacts, little is known about effective suicide prevention interventions in these contexts. We conducted a systematic review to examine the outcomes of suicide prevention strategies implemented in disasters and public health emergencies.

Methods

We searched five databases (Medline, Embase, PsycINFO, Web of Science, PTSDpubs) from inception to December 2022 for peer-reviewed quantitative studies that reported relevant intervention outcomes (changes in the frequency of suicide, suicide attempts, self-harm) for populations affected by disasters and public health emergencies. We assessed the quality of eligible studies using the Quality Assessment Tool for Quantitative Studies, and distilled review findings through narrative synthesis. The study protocol was registered with PROSPERO (CRD42021276195).

Results

Ten eligible and mostly observational studies were included in this review, which examined a range of universal, selective, and indicated interventions. Three of five studies of interventions in public health emergencies indicated the potential effectiveness and buffering effects of generic disaster related mental health support, access to urban parks, as well as the beneficial role of video-enabled tablets in facilitating treatment access and outcomes. Similarly, three of five studies of interventions in disaster contexts provided evidence of the beneficial role of universal economic security measures, national gun laws and buy back schemes, and volunteer-delivered mental health support. Overall, four of six studies with favorable outcomes examined interventions specifically deployed in disaster or public health emergency contexts, whereas two studies examined ongoing existing interventions. Three studies, respectively, of suicide prevention focused interventions or generic interventions reported favorable outcomes. The quality of included studies was variable, with two studies being rated as ‘strong’, four studies rated as ‘moderate’, and four studies rated as ‘weak’.

Conclusion

Notwithstanding the limited scope and variable quality of published evidence, our review findings highlight the breadth of interventions that have been applied in such contexts with some success. There is a need for further research on effective interventions and intervention adaptations to inform evidence-based suicide prevention responses to disasters and public health emergencies.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021276195, PROSPERO ID CRD42021276195.
Resistance exercise snacks improve muscle mass in female university employees: a prospective, controlled, intervention pilot-study
Authors: Brandt Tom , Schwandner Christian Thomas Lothar , Schmidt Annette
Published: February 2024

Background

Although resistance training (RT) is essential to preserve musculoskeletal fitness and maintain a healthy, independent life into old age, few women perform RT. We investigated whether resistance exercise snacking (RES) could be an efficient training approach for the workplace health promotion (WHP) to minimize barriers for participation and facilitate RT in women in order to improve musculoskeletal fitness.

Methods

This pilot-study followed a prospective, controlled intervention design. Female employees with sedentary occupations doing RT on less than 2 days/week before study participation were included. Participants self-selected for either intervention (IG) or control group (CG). While the IG [N = 15, mean age 42.1 (SD = 11.1) years] did 10 min of RES on working days for 12 weeks, the CG [N = 15, mean age 49.9 (SD = 9.7) years] was instructed to maintain their habitual physical activity. Primary endpoint was change in muscle mass. Secondary endpoint was change in maximum isometric strength. Balance, cardiovascular fitness, perceived health, and general life satisfaction was assessed for exploratory purpose. Measurements were taken before and after the intervention.

Results

12 participants of IG and 14 of CG completed the study. Muscle mass improved significantly more in the IG [+0.42 (SD = 0.54) kg] compared to the CG [−0.16 (SD = 0.51) kg] (p = 0.01,ƞ2p = 0.24). Strength did not change significantly between groups. Nevertheless, there was a trend for greater improvements in the IG compared to the CG for trunk extension, trunk flexion, and upper body push but not upper body pull. Regarding exploratory endpoints, no significant between-group changes were found. Despite their poor fitness, both groups perceived their health as good and had high life satisfaction before and after the intervention.

Conclusion

RES could be an effective approach for the WHP to promote RT in inactive women with sedentary occupations and improve their muscle mass.
Integrated physical and mental management for China’s elderly: insights from Long-gang District, Shenzhen
Authors: Wang Xun qiang , Yang Ce ce , Sun Xi ling
Published: February 2024

China is in a period of rapid population aging. The total population of the elderly aged 60 and above in mainland China was 264 million in 2020, and is the country with the largest elderly population in the world, which is home to 1/5 of the world’s older people. The urgency of actively coping with the aging population has never been greater, and China has raised it to the height of national strategy. To this end, China has issued several plans and projects on aging work. Many of them include multiple overlapping components. The management of physical illness and mental illness in the elderly is over-differentiated and segmented. However, it is common for older adults with complex health problems. The body and mind are inherently integrated and interact with each other, and should not be separated. There is an urgent need for integrated healthcare services for the physical and mental health of the elderly population. The national basic public health services play an important role in early detection and awareness of health problems for the elderly in community health services. This paper introduces the elderly health management services, one of the national basic public health projects, and the psychological care project for the elderly in Shenzhen, Guangdong Province, China. Taking Long-gang District’s exploration of the joint management of physical and mental health of the elderly as an example, this review discusses the difficulties of the elderly health work, and the feasibility of integrating the elderly mental health and physical health in medical care. We outlook to build an integrated platform for physical and mental health of the elderly in China. Focus on the needs of older population, strengthen community health services, build a integrative team, fully publicize and improve health literacy of the elderly, link up and down and work together, improve coordination between providers of medical care and social services. It is of great significance to construct a strong public health system for the elderly and promote the realization of the grand goal of Healthy China.
Assessing the Ability of Genetic Programming for Feature Selection in Constructing Dispatching Rules for Unrelated Machine Environments
Authors: Đurasević Marko , Jakobović Domagoj , Picek Stjepan , Mariot Luca
Published: February 2024

The automated design of dispatching rules (DRs) with genetic programming (GP) has become an important research direction in recent years. One of the most important decisions in applying GP to generate DRs is determining the features of the scheduling problem to be used during the evolution process. Unfortunately, there are no clear rules or guidelines for the design or selection of such features, and often the features are simply defined without investigating their influence on the performance of the algorithm. However, the performance of GP can depend significantly on the features provided to it, and a poor or inadequate selection of features for a given problem can result in the algorithm performing poorly. In this study, we examine in detail the features that GP should use when developing DRs for unrelated machine scheduling problems. Different types of features are investigated, and the best combination of these features is determined using two selection methods. The obtained results show that the design and selection of appropriate features are crucial for GP, as they improve the results by about 7% when only the simplest terminal nodes are used without selection. In addition, the results show that it is not possible to outperform more sophisticated manually designed DRs when only the simplest problem features are used as terminal nodes. This shows how important it is to design appropriate composite terminal nodes to produce high-quality DRs.
Comprehensive Analysis of Management Strategies for Red Palm Weevil in Date Palm Settings, Emphasizing Sensor-Based Infestation Detection
Authors: Mendel Zvi , Voet Hillary , Nazarian Ilan , Dobrinin Svetlana , Ment Dana
Published: February 2024

The red palm weevil (Rhynchophorus ferrugineus) inflicts widespread damage in date palm plantations and urban settings, leading to stand loss and safety concerns, intensified by the economic and ecological burdens of synthetic preventive treatments. A novel approach emphasizes detecting weevil-infested palms through sensors and targeting control efforts at colonized trees. This study analyzes two datasets: field manager-reported results from settings with preventive pest control versus seismic sensor-monitored units. Sensor data translate into damage index values, exploring curative treatments, attack likelihood based on infestation history, and the impact of sensor-based management on infestation incidence decline. Findings reveal lower weevil infestation in urban areas and preventive-treated palm settings. A strong correlation exists between sensor indications and grower decisions in date plantations. Palms previously infested are more prone to damage. Long-term sensor-based management reduces weevil-affected palm numbers significantly. The high infestation in sensor-equipped date palm settings stems from a gap between sensor-identified and preventively treated palms. Controlling weevils based on sensor indications aligns practically with grower curative treatments, preventing significant damage. Weevils target previously infested palms, optimizing pest control through a continuous strategy integrating sensor monitoring with prompt response treatments. This proves highly effective, offering a cost-efficient alternative to preventive treatment reliance.
IPMCNet: A Lightweight Algorithm for Invasive Plant Multiclassification
Authors: Chen Ying , Qiao Xi , Qin Feng , Huang Hongtao , Liu Bo , Li Zaiyuan , Liu Conghui , Wang Quan , Wan Fanghao , Qian Wanqiang , Huang Yiqi
Published: February 2024

Invasive plant species pose significant biodiversity and ecosystem threats. Real-time identification of invasive plants is a crucial prerequisite for early and timely prevention. While deep learning has shown promising results in plant recognition, the use of deep learning models often involve a large number of parameters and high data requirements for training. Unfortunately, the available data for various invasive plant species are often limited. To address this challenge, this study proposes a lightweight deep learning model called IPMCNet for the identification of multiple invasive plant species. IPMCNet attains high recognition accuracy even with limited data and exhibits strong generalizability. Simultaneously, by employing depth-wise separable convolutional kernels, splitting channels, and eliminating fully connected layer, the model’s parameter count is lower than that of some existing lightweight models. Additionally, the study explores the impact of different loss functions, and the insertion of various attention modules on the model’s accuracy. The experimental results reveal that, compared with eight other existing neural network models, IPMCNet achieves the highest classification accuracy of 94.52%. Furthermore, the findings suggest that focal loss is the most effective loss function. The performance of the six attention modules is suboptimal, and their insertion leads to a decrease in model accuracy.
Effects of Heat Stress and Lipopolysaccharides on Gene Expression in Chicken Immune Cells
Authors: Yang Guang , Zhou Xinyi , Chen Shutao , Liu Anfang , Liu Lingbin , Wang Haiwei , Wang Qigui , Lan Xi
Published: February 2024

Prolonged exposure to high temperatures and humidity can trigger heat stress in animals, leading to subsequent immune suppression. Lipopolysaccharides (LPSs) act as upstream regulators closely linked to heat stress, contributing to their immunosuppressive effects. After an initial examination of transcriptome sequencing data from individual samples, 48 genes displaying interactions were found to potentially be associated with heat stress. Subsequently, to delve deeper into this association, we gathered chicken bone marrow dendritic cells (BMDCs). We combined heat stress with lipopolysaccharides and utilized a 48 × 48 Fluidigm IFC quantitative microarray to analyze the patterns of gene changes under various treatment conditions. The results of the study revealed that the combination of heat stress and LPSs in a coinfection led to reduced expressions of CRHR1, MEOX1, and MOV10L1. These differentially expressed genes triggered a pro-inflammatory response within cells via the MAPK and IL-17 signaling pathways. This response, in turn, affected the intensity and duration of inflammation when experiencing synergistic stimulation. Therefore, LPSs exacerbate the immunosuppressive effects of heat stress and prolong cellular adaptation to stress. The combination of heat stress and LPS stimulation induced a cellular inflammatory response through pathways involving cAMP, IL-17, MAPK, and others, consequently leading to decreased expression levels of CRHR1, MEOX1, and MOV10L1.
Discrepancies between Genetic and Visual Coat Color Assignment in Sarcidano Horse
Authors: Mura Maria Consuelo , Carcangiu Vincenzo , Cosso Giovanni , Columbano Nicolò , Sanna Passino Eraldo , Luridiana Sebastiano
Published: February 2024

This study aimed to evaluate the discrepancies between genetic and visual coat color assignment in the Sarcidano Horse and to elucidate potential reasons. Individual DNA from 90 Sarcidano Horses was used for genetic assignment of coat color to explore the correspondence with individual forms containing phenotypical traits. The MC1R exon 1 and ASIP exon 3 have been genotyped and sequenced to obtain a picture of the coat color distribution in this breed. Surprisingly, once we compared the genetic results with the individual forms reporting the phenotypic data for each subject, a certain degree of non-correspondence between the phenotypic and genetic data in relation to coat color emerged. From the genetic analysis, Chestnuts (n = 58) resulted the most common Sarcidano Horse (n = 58), followed by a quite large number of Blacks (n = 28) and a very small number of Bays (n = 4), whereas phenotypic distribution resulted in 38 Chestnuts, 40 Bays, only 2 Blacks, and 10 Grays (without the possibility of recognizing the true color they carried). Chestnut resulted a very representative coat color, while many horses that visually identified as Bays were genetically Blacks. This discrepancy, that could be due to a variety of individual and external factors, including age, time of year, living situation and dietary condition, suggesting the importance of accurate coat color identification to ensure adequate features registration and reliable prediction of offspring’s coat color.
Age-Dependent Changes in Nrf2/Keap1 and Target Antioxidant Protein Expression Correlate to Lipoxidative Adducts, and Are Modulated by Dietary N-3 LCPUFA in the Hippocampus of Mice
Authors: Díaz Mario , Valdés-Baizabal Catalina , de Pablo Daniel Pereda , Marin Raquel
Published: February 2024

The brain has a high metabolism rate that may generate reactive oxygen and nitrogen species. Consequently, nerve cells require highly efficient antioxidant defenses in order to prevent a condition of deleterious oxidative stress. This is particularly relevant in the hippocampus, a highly complex cerebral area involved in processing superior cognitive functions. Most current evidence points to hippocampal oxidative damage as a causal effect for neurodegenerative disorders, especially Alzheimer’s disease. Nuclear factor erythroid-2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2/Keap1) is a master key for the transcriptional regulation of antioxidant and detoxifying systems. It is ubiquitously expressed in brain areas, mainly supporting glial cells. In the present study, we have analyzed the relationships between Nrf2 and Keap1 isoforms in hippocampal tissue in response to aging and dietary long-chain polyunsaturated fatty acids (LCPUFA) supplementation. The possible involvement of lipoxidative and nitrosative by-products in the dynamics of the Nrf2/Keap1 complex was examined though determination of protein adducts, namely malondialdehyde (MDA), 4-hydroxynonenal (HNE), and 3-nitro-tyrosine (NTyr) under basal conditions. The results were correlated to the expression of target proteins heme-oxygenase-1 (HO-1) and glutathione peroxidase 4 (GPx4), whose expressions are known to be regulated by Nrf2/Keap1 signaling activation. All variables in this study were obtained simultaneously from the same preparations, allowing multivariate approaches. The results demonstrate a complex modification of the protein expression patterns together with the formation of adducts in response to aging and diet supplementation. Both parameters exhibited a strong interaction. Noticeably, LCPUFA supplementation to aged animals restored the Nrf2/Keap1/target protein patterns to the status observed in young animals, therefore driving a “rejuvenation” of hippocampal antioxidant defense.
Molecular Mechanism of Natural Food Antioxidants to Regulate ROS in Treating Cancer: A Review
Authors: Muchtaridi Muchtaridi , Az-Zahra Farhah , Wongso Hendris , Setyawati Luthfi Utami , Novitasari Dhania , Ikram Emmy Hainida Khairul
Published: February 2024

Cancer is the second-highest mortality rate disease worldwide, and it has been estimated that cancer will increase by up to 20 million cases yearly by 2030. There are various options of treatment for cancer, including surgery, radiotherapy, and chemotherapy. All of these options have damaging adverse effects that can reduce the patient’s quality of life. Cancer itself arises from a series of mutations in normal cells that generate the ability to divide uncontrollably. This cell mutation can happen as a result of DNA damage induced by the high concentration of ROS in normal cells. High levels of reactive oxygen species (ROS) can cause oxidative stress, which can initiate cancer cell proliferation. On the other hand, the cytotoxic effect from elevated ROS levels can be utilized as anticancer therapy. Some bioactive compounds from natural foods such as fruit, vegetables, herbs, honey, and many more have been identified as a promising source of natural antioxidants that can prevent oxidative stress by regulating the level of ROS in the body. In this review, we have highlighted and discussed the benefits of various natural antioxidant compounds from natural foods that can regulate reactive oxygen species through various pathways.
A Regional Brightness Control Method for a Beam Projector to Avoid Human Glare
Authors: Jeon Hyeong-Gi , Lee Kyoung-Hee
Published: February 2024

In this study, we proposed a system to reduce the speaker’s suffering from the strong light of a beam projector by applying regional brightness control over the screen. Since the original image and the projected one on the screen are quite different in area, brightness, and color, the proposed system first transforms them so that they have the same area and similar color tone. Then, to accurately determine the difference between those images, we have introduced a SSIM map, which is a perception-based method of measuring image similarity. Accordingly, an image segmentation model is used to determine the speaker’s silhouette from the SSIM map. We applied a couple of well-trained segmentation models, such as Selfie and DeepLab-v3, provided with MediaPipe. The experimental results showed the operability of the proposed system and that it determines most of a lecturer’s body area on the screen. To closely evaluate the system’s effectiveness, we have measured error rates consisting of false-positive and false-negative errors in the confusion matrix. With the measured results, the error rates appeared so insignificant and stable that the proposed system provides a practical effect for the speakers, especially in the case of applying DeepLab-v3. With the results, it is implied that an accurate segmentation model can considerably elevate the effectiveness of the system.
Choreographing Social Memories: Healing and Collective Imagining in Eiko Otake and Wen Hui’s Artistic Collaboration
Authors: Guan Jingqiu
Published: February 2024

This article explores the first-time choreographic collaboration between Eiko Otake, a renowned Japanese dance artist, and Wen Hui, a celebrated Chinese choreographer and filmmaker, which took place in mainland China in January of 2020. The outbreak of the coronavirus in Wuhan compelled Otake to return to the US prematurely, and the subsequent global pandemic led the two artists to continue working together through the computer screen. Constructed from daily footage of Wen and Otake moving together, conversing about their personal histories and choreographic works, and visiting the Nanjing Massacre Memorial, the resulting documentary film No Rule Is Our Rule (2023, 74 min) offers a poignant portrayal of their creative process, which places utmost importance on honesty and openness. Through an in-depth analysis of their artistic exploration presented through the film, the article examines how their collaborative endeavor which prioritizes corporeal interaction and unfiltered dialogues can be conceived as a form of mediated social choreography. I argue that their embodied methodology, grounded in the interweaving of personal and social memories, points to the potential for collective healing from the tension and trauma in Sino-Japanese history and promotes collective imagining through intercultural dialogues.
Analysis of the Micro-Physical Characteristics of the Sea of Clouds Phenomena in Jiuxian Mountain Based on Multiple Source Observations
Authors: Cheng Si , Lin Zilun , Zhou Jianding , Han Geng , Chen Zhenhao , Yang Qingbo
Published: February 2024

The micro-physical characteristics of a typical sea of clouds process in Jiuxian Mountain are investigated by comprehensively analyzing parameters that delineate the micro-physical characteristics of clouds and atmospheric stratification based on data from a cloud radar, wind profiler, meteorological gradient observation in high mountains, and other observations. The results show that water vapor condenses into cloud particles via an entrained and mixing process accompanied by an updraft originating from orographic uplift. During the thickening stage of the sea of clouds, atmospheric motion within the clouds is featured as “downdraft on the top—updraft on the bottom”. The zero vertical velocity area is located closely to the maximum of liquid water content. The thermal inversion layer is formed during the maintenance stage; however, the enhancement of inversion on the cloud top could suppress updraft in areas with a high liquid water content. The values mainly concentrate on the cloud top, and repetitively lifting and falling processes caused by the atmospheric upward and downward motion are in favor of the coalescence growth of cloud particles, which result in the persistence of strong radar echo. At the dissipation stage, warming on the cloud top is greater than that on the cloud bottom due to the short-wave absorption of clouds as the solar radiation enhances. As a result, the inversion layer thickens and elevates, evaporation caused by heating outweighs the condensation caused by cooling, a strong radar echo band descends from the top to the middle part of clouds, a sea of clouds dissipates gradually as cloud particles evaporates, and the particle size and concentration number of cloud particles decrease simultaneously.
Distribution of Acetogenic Naphthoquinones in Droseraceae and Their Chemotaxonomic Utility
Authors: Schlauer Jan , Fleischmann Andreas , Hartmeyer Siegfried R. H. , Hartmeyer Irmgard , Rischer Heiko
Published: February 2024

Chemotaxonomy is the link between the state of the art in analytical chemistry and the systematic classification and phylogenetic analysis of biota. Although the characteristic secondary metabolites from diverse biotic sources have been used in pharmacology and biological systematics since the dawn of mankind, only comparatively recently established reproducible methods have allowed the precise identification and distinction of structurally similar compounds. Reliable, rapid screening methods like TLC (Thin Layer Chromatography) can be used to investigate sufficiently large numbers of samples for chemotaxonomic purposes. Using distribution patterns of mutually exclusive naphthoquinones, it is demonstrated in this review how a simple set of chemical data from a representative sample of closely related species in the sundew family (Droseraceae, Nepenthales) provides taxonomically and phylogenetically informative signal within the investigated group and beyond.
A Review: Multi-Omics Approach to Studying the Association between Ionizing Radiation Effects on Biological Aging
Authors: Ruprecht Nathan A. , Singhal Sonalika , Schaefer Kalli , Panda Om , Sens Donald , Singhal Sandeep K.
Published: February 2024

Multi-omics studies have emerged as powerful tools for tailoring individualized responses to various conditions, capitalizing on genome sequencing technologies’ increasing affordability and efficiency. This paper delves into the potential of multi-omics in deepening our understanding of biological age, examining the techniques available in light of evolving technology and computational models. The primary objective is to review the relationship between ionizing radiation and biological age, exploring a wide array of functional, physiological, and psychological parameters. This comprehensive review draws upon an extensive range of sources, including peer-reviewed journal articles, government documents, and reputable websites. The literature review spans from fundamental insights into radiation effects to the latest developments in aging research. Ionizing radiation exerts its influence through direct mechanisms, notably single- and double-strand DNA breaks and cross links, along with other critical cellular events. The cumulative impact of DNA damage forms the foundation for the intricate process of natural aging, intersecting with numerous diseases and pivotal biomarkers. Furthermore, there is a resurgence of interest in ionizing radiation research from various organizations and countries, reinvigorating its importance as a key contributor to the study of biological age. Biological age serves as a vital reference point for the monitoring and mitigation of the effects of various stressors, including ionizing radiation. Ionizing radiation emerges as a potent candidate for modeling the separation of biological age from chronological age, offering a promising avenue for tailoring protocols across diverse fields, including the rigorous demands of space exploration.
Contextual Priors Shape Action Understanding before and beyond the Unfolding of Movement Kinematics
Authors: Bianco Valentina , Finisguerra Alessandra , Urgesi Cosimo
Published: February 2024

Previous studies have shown that contextual information may aid in guessing the intention underlying others’ actions in conditions of perceptual ambiguity. Here, we aimed to evaluate the temporal deployment of contextual influence on action prediction with increasing availability of kinematic information during the observation of ongoing actions. We used action videos depicting an actor grasping an object placed on a container to perform individual or interpersonal actions featuring different kinematic profiles. Crucially, the container could be of different colors. First, in a familiarization phase, the probability of co-occurrence between each action kinematics and color cues was implicitly manipulated to 80% and 20%, thus generating contextual priors. Then, in a testing phase, participants were asked to predict action outcome when the same action videos were occluded at five different timeframes of the entire movement, ranging from when the actor was still to when the grasp of the object was fully accomplished. In this phase, all possible action–contextual cues’ associations were equally presented. The results showed that for all occlusion intervals, action prediction was more facilitated when action kinematics deployed in high- than low-probability contextual scenarios. Importantly, contextual priors shaped action prediction even in the latest occlusion intervals, where the kinematic cues clearly unveiled an action outcome that was previously associated with low-probability scenarios. These residual contextual effects were stronger in individuals with higher subclinical autistic traits. Our findings highlight the relative contribution of kinematic and contextual information to action understanding and provide evidence in favor of their continuous integration during action observation.
Centralized or Decentralized? Communication Network and Collective Effectiveness of PBOs—A Task Urgency Perspective
Authors: Ding Xue , Shen Wenxin , Wang Shiai
Published: February 2024

In the construction industry, there are a large number of project-based organizations (PBOs), where the efficiency of communication and collaboration among organizational members greatly impacts the success of projects. For PBOs employing both centralized and decentralized communication networks, it is worth delving into the question of under what circumstances which type of network will yield better results. Based on the IMO model and organizational learning theory, this paper conducts a grouped communication experiment involving 598 engineering management personnel to explore the differences in collective effectiveness of varying communication networks from the perspective of task urgency. Beyond task performance assessments, we have included organizational member perception to form evaluation criteria for collective effectiveness. Our research results show that under conditions of weak task urgency, decentralized networks yield higher collective effectiveness. Conversely, under conditions of strong task urgency, centralized networks demonstrate superior collective effectiveness. Furthermore, this study also verifies the mediating role of knowledge sharing behavior when task urgency is strong. This research provides significant managerial insights for the establishment of appropriate communication networks for PBOs in the construction industry.
Extra-Capsular Spread of Lymph Node Metastasis in Oral, Oropharyngeal and Hypopharyngeal Cancer: A Comparative Subsite Analysis
Authors: Kang Yung Jee , Park Goeun , Park Sung Yool , Kim Taehwan , Kim Eunhye , Heo Yujin , Lee Changhee , Jeong Han-Sin
Published: February 2024

Background: The extra-capsular spread (ECS) of lymph node metastasis (LNM) is a hallmark of aggressive primary tumor phenotype in head and neck cancer (HNC); however, the factors influencing ECS are poorly understood. Patients and Methods: This was a retrospective study, including 190 cases of oral tongue cancer (OTC), 148 cases of oropharyngeal cancer (OPC) (118 HPV-positive and 30 HPV-negative), and 100 cases of hypopharyngeal cancer (HPC). Tumor dimension, tumor biological variables (lymphovascular/perineural invasion and histologic grade), and LNM variables (LNM number and size) were analyzed according to the presence of ECS using multivariable logistic regression and receiver operating characteristic (ROC) curve analyses. Results: LNM variables were significant factors for ECS in all subsites of HNC (p < 0.05), except HPV-positive OPC. In OTC, tumor dimensional variables were significantly related to ECS (p < 0.01). Meanwhile, in OPC and HPC, neither the primary tumor dimension nor the T status were significant factors for ECS occurrence. The predictability of ECS by ROC curve using multiple variables was 0.819 [95% confidence interval: 0.759–0.878] in OTC, 0.687 [0.559–0.815] in HPV-positive OPC, 0.823 [0.642–1.000] in HPV-negative OPC, and 0.907 [0.841–0.973] in HPC. Conclusion: LNM variables were correlated with ECS occurrence for most HNC subsites, and site-dependent primary tumor characteristics might contribute differentially to the ECS development of LNM in HNC.
Insight into Recent Advances in Degrading Androgen Receptor for Castration-Resistant Prostate Cancer
Authors: Chen Qiao-Hong , Munoz Erick , Ashong Dennis
Published: February 2024

Induced protein degradation has emerged as an innovative drug discovery approach, complementary to the classical method of suppressing protein function. The androgen receptor signaling pathway has been identified as the primary driving force in the development and progression of lethal castration-resistant prostate cancer. Since androgen receptor degraders function differently from androgen receptor antagonists, they hold the promise to overcome the drug resistance challenges faced by current therapeutics. Proteolysis-targeting chimeras (PROTACs), monomeric degraders, hydrophobic tagging, molecular glues, and autophagic degradation have demonstrated their capability in downregulating intracellular androgen receptor concentrations. The potential of these androgen receptor degraders to treat castration-resistant prostate cancer is substantiated by the advancement of six PROTACs and two monomeric androgen receptor degraders into phase I or II clinical trials. Although the chemical structures, in vitro and in vivo data, and degradation mechanisms of androgen receptor degraders have been reviewed, it is crucial to stay updated on recent advances in this field as novel androgen receptor degraders and new strategies continue to emerge. This review thus provides insight into recent advancements in this paradigm, offering an overview of the progress made since 2020.
Bortezomib in Combination with Physachenolide C Reduces the Tumorigenic Properties of KRASmut/P53mut Lung Cancer Cells by Inhibiting c-FLIP
Authors: Kanagasabai Thanigaivelan , Dunbar Zerick , Ochoa Salvador González , Farris Tonie , Dhandayuthapani Sivanesan , Wijeratne E. M. Kithsiri , Gunatilaka A. A. Leslie , Shanker Anil
Published: February 2024

Background: Defects in apoptosis regulation are one of the classical features of cancer cells, often associated with more aggressiveness and failure to therapeutic options. We investigated the combinatorial antitumor effects of a natural product, physachenolide C (PCC) and bortezomib, in KRASmut/P53mut lung cancer cells and xenograft mice models. Methods: The in vitro anticancer effects of the bortezomib and PCC combination were investigated using cell viability, migration, and invasion assays in 344SQ, H23, and H358 cell lines. Furthermore, the effects of combination treatment on the critical parameters of cellular metabolism, including extracellular acidification rate (ECAR) and mitochondrial oxidative phosphorylation based on the oxygen consumption rate of cancer cells were assessed using Seahorse assay. Finally, the antitumor effect of the bortezomib (1 mg/kg) and PCC (10 mg/kg) combination was evaluated using xenograft mice models. Results: Our data showed that the bortezomib–PCC combination was more effective in reducing the viability of lung cancer cells in comparison with the individual treatments. Similarly, the combination treatment showed a significant inhibition of cell migration and invasion of cancer cells. Additionally, the key anti-apoptotic protein c-FLIP was significantly inhibited along with a substantial reduction in the key parameters of cellular metabolism in cancer cells. Notably, the bortezomib or PCC inhibited the tumor growth compared to the control group, the tumor growth inhibition was much more effective when bortezomib was combined with PCC in tumor xenograft mice models. Conclusion: These findings demonstrate that PCC sensitizes cancer cells to bortezomib, potentially improving the antitumor effects against KRASmut/P53mut lung cancer cells, with an enhanced efficacy of combination treatments without causing significant side effects.
Real-World Data on Institutional Implementation of Screening for Mismatch Repair Deficiency and Lynch Syndrome in Endometrial Cancer Patients
Authors: Joder Carmen , Gmür Andrea , Solass Wiebke , Christe Lucine , Rabaglio Manuela , Fluri Muriel , Rau Tilman T. , Saner Flurina A. M. , Knabben Laura , Imboden Sara , Mueller Michael D. , Siegenthaler Franziska
Published: February 2024

Lynch syndrome is an inherited tumor syndrome caused by a pathogenic germline variant in DNA mismatch repair genes. As the leading cause of hereditary endometrial cancer, international guidelines recommend universal screening in women with endometrial cancer. However, testing for Lynch syndrome is not yet well established in clinical practice. The aim of this study was to evaluate adherence to our Lynch syndrome screening algorithm. A retrospective, single-center cohort study was conducted of all endometrial cancer patients undergoing surgical treatment at the Bern University Hospital, Switzerland, between 2017 and 2022. Adherence to immunohistochemical analysis of mismatch repair status, and, if indicated, to MLH1 promoter hypermethylation and to genetic counseling and testing was assessed. Of all 331 endometrial cancer patients, 102 (30.8%) were mismatch repair-deficient and 3 (0.9%) patients were diagnosed with Lynch syndrome. Overall screening adherence was 78.2%, with a notable improvement over the six years from 61.4% to 90.6%. A major reason for non-adherence was lack of provider recommendation for testing, with advanced patient age as a potential patient risk factor. Simplification of the algorithm through standardized reflex screening was recommended to provide optimal medical care for those affected and to allow for cascading testing of at-risk relatives.
Prognostic Value of Apparent Diffusion Coefficient (ADC) in Patients with Diffuse Gliomas
Authors: Bušić Marija , Rumboldt Zoran , Čerina Dora , Bušić Željko , Dolić Krešimir
Published: February 2024

This study aimed to evaluate potential posttreatment changes in ADC values within the tissue surrounding the enhancing lesion, particularly in areas not exhibiting MRI characteristics of involvement. Additionally, the objective was to investigate the correlations among ADC values, treatment response, and survival outcomes in individuals diagnosed with gliomas. This retrospective study included a total of 49 patients that underwent either stereotactic biopsy or maximal surgical resection. Histologically confirmed as Grade III or IV gliomas, all cases adhered to the 2016 and 2021 WHO classifications, with subsequent radio-chemotherapy administered post-surgery. Patients were divided into two groups: short and long survival groups. Baseline and follow-up MRI scans were obtained on a 1.5 T MRI scanner. Two ROI circles were positioned near the enhancing area, one ROI in the NAWM ipsilateral to the neoplasm and another symmetrically in the contralateral hemisphere on ADC maps. At follow-up there was a significant difference in both ipsilateral and contralateral NAWM between the two groups, −0.0857 (p = 0.004) and −0.0607 (p = 0.037), respectively. There was a weak negative correlation between survival and ADC values in ipsilateral and contralateral NAWM at the baseline with the correlation coefficient −0.328 (p = 0.02) and −0.302 (p = 0.04), respectively. The correlation was stronger at the follow-up. The findings indicate that ADC values in normal-appearing white matter (NAWM) may function as a prognostic biomarker in patients with diffuse gliomas.
Hepatoblastoma Relapse—Findings from the German HB99 Trial and the German Liver Tumor Registry
Authors: Maxwell Rebecca , Häberle Beate , Kappler Roland , von Schweinitz Dietrich , Rassner Mark , von Frowein Julia , Schmid Irene
Published: February 2024

Survival rates for HB patients have improved; however, outcomes for patients who relapse remain poor. A retrospective review of information gathered for the HB99 study and the German Liver Tumor Registry identified 25 relapse patients (6.9%, 25/362). The median time from initial diagnosis to first relapse was 13 months (range: 5–66 months). Two patients relapsed >36 months after initial diagnosis. A total of 68% (17/25) of relapses were metastatic, 24% local, and 8% combined. 67% of local relapses were alive at the last follow-up, in contrast to 53% of metastatic and 0% of combined relapses. At the last follow-up, 73% (8/11) of patients with lung relapses were still alive (0/4 with peritoneal, 1/2 with CNS involvement). A total of 20% of the patients had AFP-negative relapses, 64% of the relapse patients achieved a second complete remission, 69% were still in complete second remission at the last follow-up (median FU of 66 months), and 83% (5/6) of irinotecan-naïve patients who received relapse treatment including irinotecan were in second complete remission at the last follow-up. The 3-year overall survival/event-free survival from relapse was 63%/48% respectively. There is a good chance that HB patients will achieve a second remission despite a first relapse. However, patients who suffer further relapses tend to have a poorer prognosis.