bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanomaterials for application in wound Healing: current state-of-the-art and future perspectives
Authors: Shalaby Manal Aly , Anwar Mohammed Moustapha , Saeed Hesham
Published: February 2022

Nanoparticles are the gateway to the new era in drug delivery of biocompatible agents. Several products have emerged from nanomaterials in quest of developing practical wound healing dressings that are nonantigenic, antishear stress, and gas-exchange permeable. Numerous studies have isolated and characterised various wound healing nanomaterials and nanoproducts. The electrospinning of natural and synthetic materials produces fine products that can be mixed with other wound healing medications and herbs. Various produced nanomaterials are highly influential in wound healing experimental models and can be used commercially as well. This article reviewed the current state-of-the-art and briefly specified the future concerns regarding the different systems of nanomaterials in wound healing (i.e., inorganic nanomaterials, organic and hybrid nanomaterials, and nanofibers). This review may be a comprehensive guidance to help health care professionals identify the proper wound healing materials to avoid the usual wound complications.
On the semantics of number morphology
Authors: Scontras Gregory
Published: February 2022

This paper develops a semantic account of morphological number marking, specifically in the presence of numerals. In addition to accounting for number marking on basic nouns like book in English, the account handles variation in patterns of number marking along two dimensions: cross-linguistically, between languages that either necessitate or prohibit singular morphology in the presence of numerals greater than ‘one’; and within one and the same language on the various nominal elements in English. Building off the presuppositional approach to morphological number from Sauerland (Proc SALT 13:258–275, 2003), the proposed semantics accounts for both sorts of variation by assuming flexibility in the selection of the measure relevant to the one-ness presupposition of the morphological singular form.
Intensive care unit occupancy predictions in the COVID-19 pandemic based on age-structured modelling and differential flatness
Authors: Hametner Christoph , Böhler Lukas , Kozek Martin , Bartlechner Johanna , Ecker Oliver , Du Zhang Peng , Kölbl Robert , Bergmann Michael , Bachleitner-Hofmann Thomas , Jakubek Stefan
Published: February 2022

The COVID-19 pandemic confronts governments and their health systems with great challenges for disease management. In many countries, hospitalization and in particular ICU occupancy is the primary measure for policy makers to decide on possible non-pharmaceutical interventions. In this paper a combined methodology for the prediction of COVID-19 case numbers, case-specific hospitalization and ICU admission rates as well as hospital and ICU occupancies is proposed. To this end, we employ differential flatness to provide estimates of the states of an epidemiological compartmental model and estimates of the unknown exogenous inputs driving its nonlinear dynamics. A main advantage of this method is that it requires the reported infection cases as the only data source. As vaccination rates and case-specific ICU rates are both strongly age-dependent, specifically an age-structured compartmental model is proposed to estimate and predict the spread of the epidemic across different age groups. By utilizing these predictions, case-specific hospitalization and case-specific ICU rates are subsequently estimated using deconvolution techniques. In an analysis of various countries we demonstrate how the methodology is able to produce real-time state estimates and hospital/ICU occupancy predictions for several weeks thus providing a sound basis for policy makers.
Almost all positive continuous linear functionals can be extended
Authors: van Dobben de Bruyn Josse
Published: February 2022

Let F be an ordered topological vector space (over $$\mathbb {R}$$
R
) whose positive cone $$F_+$$

F
+

is weakly closed, and let $$E \subseteq F$$

E
⊆
F

be a subspace. We prove that the set of positive continuous linear functionals on E that can be extended (positively and continuously) to F is weak-$$*$$

∗

dense in the topological dual wedge $$E_+'$$

E
+
′

. Furthermore, we show that this result cannot be generalized to arbitrary positive operators, even in finite-dimensional spaces.
Search intensity, search time and prices: evidence from retail diesel markets in France
Authors: Cárdenas Jeisson , Otero Jesús , Gutiérrez Luis H.
Published: February 2022

We study the effect of price variations over time and across space on search intensity and search time by consumers in retail markets for diesel in France. The main contribution of the paper is that existing work in industrial organisation in this area has already studied the effect of such variability on the first measure of search but not on the second one. Using novel data from daily consultation measures and price information from a government-run website, we find that price dispersion across space increases search activity and the amount of time allocated to search. Furthermore, while contemporaneous price changes do not appear to influence the number of visits and time per visit to the website, several coefficients on past price changes are positive and statistically different from zero. Our results thus suggest that price dispersion and price variability play a role in inducing search by current and potential customers.
Comparison of EQ-5D-3L and 5L versions following operative fixation of closed ankle fractures
Authors: Garratt Andrew , Stavem Knut
Published: February 2022

Purpose

To undertake the first testing and comparison of measurement properties for the EuroQol EQ-5D-3L and 5L in patients with ankle problems.

Methods

The cross-sectional postal survey of 959 patients aged ≥ 18 years, who underwent surgical treatment (ORIF) for unstable and closed ankle fractures in Eastern Norway. Both the EQ-5D-3L and 5L were included in a postal questionnaire in 2015, 3–6 years post surgery. Missing data, floor and ceiling effects, and response consistency were assessed. Tests of validity included comparisons with scores for the SF-36 and widely used ankle-specific instruments. The 5L version was assessed for test–retest reliability.

Results

There were 567 (59%) respondents; 501 completed both versions and 182 (61%) the 5L retest questionnaire. The 5L outperformed the 3L in tests of data quality and classification efficiency. Correlations with scores for other instruments largely met expectations, those for the 5L being slightly higher. All 5L scores had acceptable levels of reliability. For the 5L index, the smallest detectable differences for group and individual comparisons were 0.02 and 0.20, respectively.

Conclusion

The 5L outperformed the 3L in terms of data quality, number of health states assessed and tests of validity. The 5L is recommended in research and other applications following surgery for ankle fracture but further testing including responsiveness to change is recommended at clinically relevant follow-up periods.
Terrorist attacks and bank financial stability: evidence from MENA economies
Authors: Elnahass Marwa , Marie Mohamed , Elgammal Mohammed
Published: February 2022

This study examines the impact of terrorism on bank stability, represented by bank risk and financial performance. We consider banks from 14 countries located in the Middle East and North Africa region for the period 2010–2018 using both the three-stage least-square and the generalised method of moments. The results provide strong evidence that banks located in countries with high exposure to terrorist attack exhibit low financial stability, due to high bank risk (i.e., high credit and insolvency risk). However, these banks show high financial performance (i.e., high profitability and cost efficiency), on average. Our results also show differential impacts on bank stability for countries marked as more (less) exposed to risk of attacks. For banks located in high-income-generating countries, we find that exposure to terrorism is associated with low financial performance and high credit risk, which is the opposite case for low-income-generating countries. Our results also indicate high systemic risk for listed banks operating under high terrorism risk exposure.
The Macquarie Laws of War Corpus (MQLWC): Design, Construction and Use
Authors: Lukin Annabelle , Araujo e Castro Rodrigo
Published: February 2022

This paper discusses the creation and use of the new Macquarie Laws of War Corpus (MQLWC). The corpus consists of the 110 documents of international war law stored in the International Committee of the Red Cross treaties database, starting with the 1856 Declaration Respecting Maritime Law (Paris Declaration) and ending with the most recent amendment to the Rome Statute (2019). The new MQLWC is hosted at the Sydney Corpus Lab (sydneycorpuslab.com), via its CQWeb interface, which allows for searching of frequencies, concordance lines, and collocations. The corpus can also be downloaded for offline processing in other popular concordance programs, such as #Lancsbox and AntConc. This paper introduces the corpus, describes the process of assembling the data, and explains its limitations. The paper then demonstrates some of the ways the data can be explored using the concept of 'military necessity'. The MQLWC contributes to the growing use of corpus linguistics in legal studies, and will be of particular relevance to scholars in the field of international war law.
The Interplay of Alcohol and Wellbeing in the Workplace: Combining Soft Systems Methodology and Foucauldian Approach
Authors: Osei-Nimo Samuel , Millman Cindy , Aboagye-Nimo Emmanuel
Published: February 2022

This paper focuses on the topic of alcohol and wellbeing in contemporary work organisations. It explores the relationship between stakeholders’ viewpoints regarding alcohol in the workplace and how they have shaped organisational practices regarding wellbeing. The work of Michel Foucault is used to explore these issues. The notions of power, knowledge and discipline are identified as key Foucauldian themes that offer an alternative understanding of how discourses on alcohol are shaped in the United Kingdom workplace. The paper combines certain stages of Checkland’s Soft Systems Methodology and Foucault’s Poststructuralist approach in addressing the topic. Foucault’s method of analysis, particularly archaeology and genealogy, is used to explore how and why certain discourses surrounding alcohol in the workplace become dominant over time. Qualitative cases with semi-structured interviews in knowledge-intensive firms were adopted to capture contrasting, varied experiences and perceptions of these organisational actors and shed light on alcohol and wellbeing and its relationships with the power dimension.
Are ABM explanations in the social sciences inevitably individualist?
Authors: Kincaid Harold , Zahle Julie
Published: February 2022

Agent-based models (ABMs) are increasingly important in social science research. They have two obvious apparent virtues: they can model complex macrosociological phenomena without strong assumptions about agents and without analytic solutions for models, and they seem to instantiate the methodological individualist program in a concrete way. We argue that the latter claim is false. After providing schematic accounts of ABM models and a first introduction to ways in which to characterize individualist explanations, we work through six conceptions of individualist explanations that are decreasingly "less individualist" and argue that ABM-based explanations in the social sciences are not inevitably individualist in any of these senses. ABMs allow for explanatory relations among social entities and properties in the model environment and they are silent on what basic agents are, allowing social entities and properties to play basic explanatory roles.
Purinoceptor: a novel target for hypertension
Authors: Li Xuan , Zhu Li-juan , Lv Jing , Cao Xin
Published: February 2022

Hypertension is the leading cause of morbidity and mortality globally among all cardiovascular diseases. Purinergic signalling plays a crucial role in hypertension through the sympathetic nerve system, neurons in the brain stem, carotid body, endothelium, immune system, renin-angiotensin system, sodium excretion, epithelial sodium channel activity (ENaC), and renal autoregulation. Under hypertension, adenosine triphosphate (ATP) is released as a cotransmitter from the sympathetic nerve. It mediates vascular tone mainly through P2X1R activation on smooth muscle cells and activation of P2X4R and P2YR on endothelial cells and also via interaction with other purinoceptors, showing dual effects. P2Y1R is linked to neurogenic hypertension. P2X7R and P2Y11R are potential targets for immune-related hypertension. P2X3R located on the carotid body is the most promising novel therapeutic target for hypertension. A1R, A2AR, A2BR, and P2X7R are all related to renal autoregulation, which contribute to both renal damage and hypertension. The main focus is on the evidence addressing the involvement of purinoceptors in hypertension and therapeutic interventions.
The effects of two different 8-week stretching protocols on postural stability in amateur sportsmen
Authors: Todde F. , Carcangiu A. , Pusceddu N. , Di Giacomo A. , Tocco F.
Published: February 2022

Purpose

The purpose of this study was to compare the long-term effects of a static versus proprioceptive neuromuscular facilitation (PNF) stretching protocols by means of spinometry and baropodometry. We hypothesized that PNF may be more effective than static stretching in supporting the static and dynamic balance.

Methods

Thirty-six sport science students were divided into three groups: the first group attended a warm-up protocol on the treadmill only (warm-up group) while the other two carried out the same warm-up protocol followed by a static stretching (static group) or by a Contract Relax Antagonist Contract stretching (CRAC group). Stretching programs were performed three times per week on lower limb muscles and the postural stability of each participant was evaluated at entry (pre) and after 8 weeks of training (post).

Results

The ellipse area after a warm-up-only protocol did not change; after the static stretching protocol, it was significantly increased (from 111.3 ± 35.8 to 135.0 ± 32.3, p < 0.05), while after the CRAC protocol, it significantly decreased (from 119.1 ± 23.0 to 88.2 ± 19.8, p < 0.01). CRAC group had a significant reduction in average forces (− 3.9% from pre to post, p < 0.05) and in load response (− 0.6% from pre to post, p < 0.05). Pelvic inclination was reduced from 2.22 to 1.33 mm (p < 0.01) and pelvic torsion decreased by 0.94 ± 0.22° (p < 0.05) after the CRAC protocol.

Conclusions

Eight-week CRAC protocol was an excellent training for static and dynamic balance improvement and it was more effective than static stretching.
Sustainable application of novel herbs on cotton fabrics as biomordants and colourants
Authors: Thakker Alka Madhukar , Sun Danmei
Published: February 2022

The textile industry is ambitiously parading towards circularity and curtailing its dependency on fossil fuels hence the instigated research that adheres to Sustainable Developmental Goals (SDGs) and benefits the environment and human health alike. The current research experimented with innovative sources of plant-based biomaterials namely dandelion leaves, bilberry leaves, nettle leaves, and violet herb for application on cotton fabric as biomordants and colourants. The AAS analysis of mild 6% herbal solution revealed ferrous content of 6.78 mg/l in violet herb and 5.03 mg/l of the copper content in dandelion leaves applicable on cotton fabrics as biomordants thereby preventing the depletion of copper and ferrous elements mineral ores. A fair lightfastness rating and good to very good wash and rub fastness test results were obtained individually. The maximum K/S value of 13.95 was gained on cotton fabrics treated with a mild herbal infusion of bilberry leaves and coloured with violet herbs. The ATR-FTIR peak analysis noted strong = C-H bend of alkenes, strong C = O stretch of α and β unsaturated esters, and strong C-O stretch of carboxylic acids functional groups in raw herbs and its treated cotton fabrics. The dissolved oxygen (DO), oxidation–reduction potential (ORP), and potential of Hydrogen (pH) values were found in the acceptable range for all the spent solutions left after colouration of cotton fabrics with violet herbs hence sustainable. The data set obtained was statistically validated with ANOVA one-way test. Life cycle analysis and clinical investigation on potential therapeutic benefits of herbal fabrics to the wearer are suggested for future research and development.
Anthropogenically impacted lake catchments in Denmark reveal low microplastic pollution
Authors: Kallenbach Emilie M. F. , Friberg Nikolai , Lusher Amy , Jacobsen Dean , Hurley Rachel R.
Published: February 2022

Microplastics have been detected in lake environments globally, including in remote regions. Agricultural and populated areas are known to congregate several inputs and release pathways for microplastic. This study investigated microplastic (50–5000 µm) contamination in five Danish freshwater lakes with catchments dominated by arable land use. The concentrations in sediments (n = 3/site) and the zebra mussel, Dreissena polymorpha (n = 30/site), were calculated and compared with catchment characteristics and environmental parameters. Microplastic concentrations in sediment were relatively low (average 0.028 ± 0.017 items/g dry weight sediment) whilst only a single microplastic was found in the mussels (average 0.067 ± 0.249 items/10 individual). Hence, no relationship between the number of observed microplastics in sediment and mussels could be identified, nor could a relationship between concentration in sediment and environmental parameters. As all lakes studied received their water from moderate to heavily anthropogenically impacted catchments, it was expected that they would be sinks for microplastic with high bioavailability. Based on the results of the present study, D. polymorpha were found to not be contaminated by microplastics in the five study lakes. Thus, our results suggest that these mussels do not interact with microplastics at low concentrations. We speculate that the results on sediment and biota could be explained by several factors related to regional differences in plastic use, species characteristics, sampling size, and the fact that finding no microplastic is not always reported in the scientific literature. Thus, the paper provides insight into the dynamics between the catchment, lake, and biota in systems with low microplastic concentration.
Alleviation of nicotine-induced reproductive disorder, clastogenicity, and histopathological alterations by fenugreek saponin bulk and nanoparticles in male rats
Authors: Hamed Karima A. , El-Fiky Samia A. , Gawish Azza M. , Khalil Wagdy K. B. , Mohamed Hanan R. H.
Published: February 2022

Nicotine is the most abundant ingredient in cigarette smoking and has serious side effects on the lung, heart, reproductive system, and many other human organs. Saponins extracted from many plants exhibit multiple biological actions such as anti-cancer effects. Therefore, the possible protective effect of fenugreek saponin (FS) and nanofenugreek saponin (NFS) against nicotine-induced toxicity in male rats was investigated in this study. Animals were divided into a control group and the nicotine (1.5 mg/kg/day), FS (25, 50, and 100 mg/kg/day), or/and NFS (20, 40, and 80 mg/kg/day) administered groups. Micronucleus assay, histopathological, and sperm abnormality examinations as well as measurement of the acetylcholinesterase (AChE) gene expression were conducted. Our findings revealed that nicotine treatment induced significant increases in the incidence of micronucleus, sperm abnormalities, and expression levels of AChE in addition to inducing histopathological changes in rat testis. On the other hand, administration of FS or NFS with nicotine significantly decreased the incidence of micronuclei and the percentage of sperm abnormalities as well as the expression levels of AChE gene. Moreover, nicotine-induced histological alterations were reduced by given FS or NFS with nicotine. In conclusion, nicotine-induced sperm abnormalities, chromosomal damage, and histological injuries were mitigated by administration of FS or NFS with nicotine, and thus, FS and NFS could be used as ameliorating agents against nicotine toxicity.

Graphical abstract
Reuse of lead glass sludge in the fabrication of thermally insulating foamed glass with outstanding properties and high Pb-stabilization
Authors: Abdel-Gawwad Hamdy A. , Mohammed Mona S. , Arif Mohammed A. , Shoukry Hamada
Published: February 2022

This study represents the sustainable/safe consumption of lead glass sludge (LGS) in the fabrication of thermally insulating foamed glass via sintering (750–950º C) and chlorination processes. The impact of selected additives including calcium chloride (CaCl2) and sodium hydroxide (NaOH) on the foaming efficiency and Pb-stabilization has been deeply investigated. LGS is mainly lead silicate material with considerable content of calcium carbonate, which acts as foaming agent during sintering process. The newly developed foamed-materials exhibited thermal conductivity of 0.054–0.136 W/m.K, density of 0.23–1.10 g/cm3, porosity of 63.3–92.6%, and compressive strength of 0.10–2.69 MPa. X-ray diffraction proved that the immobilization mechanism was attributed to the transformation of free Pb within LGS into insoluble ganomalite Pb9Ca5MnSi9O33 phase. Adding NaOH enhanced the foaming process accompanied by a significant reduction in Pb-leaching. Incorporating CaCl2 has resulted in a retardation in Pb-leaching, which associated with Pb-stabilization and Pb-vaporization. In an attempt to reduce CO2-emission, the potential use of alkali-rich-wastewater (AW) as eco-friendly alkali source in lieu of NaOH was studied. Regardless of the variation in Pb-concentrations in leachates, all samples recorded Pb-concentrations lower than the safe limit (≤ 5 mg/l), achieving Pb-immobilization of 95.98–99.87%. The significantly reduced thermal conductivity and enhanced Pb-immobilization efficiency along with the reasonable compressive strength summarize the major innovation presented in this study.
Managing e-waste from a closed-loop lifecycle perspective: China’s challenges and fund policy redesign
Authors: Tian Tingting , Liu Guangfu , Yasemi Hussein , Liu Yang
Published: February 2022

E-waste is one of the fastest growing streams of solid waste globally, and its effective management has become a focused issue, which requires a deep understanding of the core guiding theory of extended producer responsibility (EPR). Over the past 20 years, China, one of the world’s largest producers of electrical and electronic equipment (EEE), has made great efforts to improve e-waste management along with the massive generation of e-waste. In 2012, China implemented a unique EPR-based e-waste fund policy. However, the fund policy is unsustainable due to the challenges of non-closed resource use, informal recycling, and fund imbalance. Beginning with an overview of these challenges, this paper focuses on redesigning the fund policy from a closed-loop lifecycle perspective in order to maintain a balanced development of the resource use loop and the fund system in China’s ten-year plan. In doing so, two EPR instruments, recycling content standards and consumer-oriented deposits, are added to the current fund policy. Subsequently, three extension scenarios alternately changed a critical parameter of the model to test the impact on sustainable capabilities. In this way, the sustainable supply of funds and secondary resources for the e-waste industry can be established in China and effectively demonstrate solid waste management in developing countries.
Designing and delivering representation-focused science lessons in a digital learning environment
Authors: Cirkony Connie , Tytler Russell , Hubber Peter
Published: February 2022

Inquiry-based representation-focused approaches in science education have shown promising outcomes when students engage in knowledge building via an active process of constructing, coordinating, and evaluating representations. To date, much of the existing research around these approaches has taken place in pre-digital classrooms, but the increasing importance of digital technologies in contemporary science classrooms that has heightened during the on-going Covid pandemic could have ramifications for such approaches. To explore the potential for productive integration of a digital technology with active learning processes, this study investigated the role an interactive online learning platform played in supporting the construction and refinement of representations by students. This paper draws on research generated in the context of an Australian Grade 9 science class studying a physics unit focused on energy transfer considerations for sustainable housing. The research design featured an exploratory case study approach using multiple methods for data collection including video capture, interviews, and student artefacts. Data analysis involved the application of socio-semiotic perspectives to understand the nature of students’ meaning-making processes through their generation and coordination of both digital and non-digital multimodal representations. This study found that the online platform allowed for extended access and the flexible use of multimodal resources facilitated students’ representation construction activities. However, activities involving teacher-guided discussions and ongoing feedback were limited. The socio-semiotic analysis provided insights into the effective design of online learning sequences to guide conceptual development. This paper concludes by suggesting how specific affordances of interactive online platforms can effectively facilitate the design and delivery of active learning approaches involving representation construction.
Implementation of the flipped classroom and its longitudinal impact on improving academic performance
Authors: Torres-Martín César , Acal Christian , El-Homrani Mohammed , Mingorance-Estrada Ángel C.
Published: February 2022

The objective has been to know the impact of the flipped classroom methodology on the academic performance of students during their training process in relation to the traditional methodology over time, in order to establish baselines in the academic grades in both models. The research is of a quasi-experimental type of non-equivalent groups, with a longitudinal trend design in the data collection process. The entire available population has been selected, with 1.236 students participating, exploring the grades as an analytical resource, from the 2010/2011 to the 2019/2020 academic years. The results show statistically significant differences in the improvement of academic performance with the flipped classroom methodology. Furthermore, the results reinforce that the flipped teaching model effectively promotes students’ interest, their capacity for autonomous learning and personal and cooperative relationships.
Effect of Nb and Mo on Austenite Microstructural Evolution During Hot Deformation in Boron High Strength Steels
Authors: Zurutuza Irati , Isasti Nerea , Detemple Eric , Schwinn Volker , Mohrbacher Hardy , Uranga Pello
Published: February 2022

This work has focused on the study of hot working behavior of boron high strength steels microalloyed with different combinations of Nb and/or Mo. The role of Nb and Mo during the hot deformation of low carbon steels is well known: both mainly retard austenite recrystallization, leading to pancaked austenite microstructures before phase transformation and to refined room temperature microstructures. However, the design of rolling schedules resulting in properly conditioned microstructures, requires microstructural evolution models that take into account the effect of the different alloying elements. In this specific case, the effect that high levels of molybdenum (0.5 pct) have in the recrystallization delay was evaluated. In that respect, hot torsion tests were performed in this work to investigate the microstructural evolution during hot deformation of four boron steels, with different Nb (0.025 pct) and Mo (0.5 pct) combinations. The retardation in recrystallization kinetics was modeled in all cases and measured kinetics agree with those predicted by equations previously developed for Nb–Mo microalloyed steels with lower Mo concentrations (< 0.3 pct). The strain-induced precipitation in the Nb and Nb–Mo bearing steels was also characterized. Finally, the fractional softening evolution during multipass rolling simulations was compared with MicroSim® model predictions, showing a good agreement with experimental results.
Eight versus 28-point lung ultrasonography in moderate acute heart failure: a prospective comparative study
Authors: Leidi Antonio , Soret Guillaume , Mann Tamara , Koegler Flora , Coen Matteo , Leszek Alexandre , Dubouchet Laetitia , Guillermin Alexandre , Kaddour Myriam , Rouyer Frédéric , Combescure Christophe , Carballo Sebastian , Reny Jean-Luc , Marti Christophe , Stirnemann Jérôme , Grosgurin Olivier
Published: February 2022

Lung ultrasonography (LUS) is an accurate method of estimating lung congestion but there is ongoing debate on the optimal number of scanning points. The aim of the present study was to compare the reproducibility (i.e. interobserver agreement) and the feasibility (i.e. time consumption) of the two most practiced protocols in patients hospitalized for acute heart failure (AHF). This prospective trial compared 8- and 28-point LUS protocols. Both were performed by an expert–novice pair of sonographers at admission and after 4 to 6 days on patients admitted for AHF. A structured bio-clinical evaluation was simultaneously carried out by the treating physician. The primary outcome was expert-novice interobserver agreement estimated by kappa statistics. Secondary outcomes included time spent on image acquisition and interpretation. During the study period, 43 patients underwent a total of 319 LUS exams. Expert–novice interobserver agreement was moderate at admission and substantial at follow-up for 8-point protocol (weighted kappa of 0.54 and 0.62, respectively) with no significant difference for 28-point protocol (weighted kappa of 0.51 and 0.41; P value for comparison 0.74 at admission and 0.13 at follow-up). The 8-point protocol required significantly less time for image acquisition at admission (mean time difference − 3.6 min for experts, − 5.1 min for novices) and interpretation (− 6.0 min for experts and − 6.3 min for novices; P value < 0.001 for all time comparisons). Similar differences were observed at follow-up. In conclusion, an 8-point LUS protocol was shown to be timesaving with similar reproducibility when compared with a 28-point protocol. It should be preferred for evaluating lung congestion in AHF inpatients.
SARS-CoV-2 Infects Red Blood Cell Progenitors and Dysregulates Hemoglobin and Iron Metabolism
Authors: Kronstein-Wiedemann Romy , Stadtmüller Marlena , Traikov Sofia , Georgi Mandy , Teichert Madeleine , Yosef Hesham , Wallenborn Jan , Karl Andreas , Schütze Karin , Wagner Michael , El-Armouche Ali , Tonn Torsten
Published: February 2022

Background

SARS-CoV-2 infection causes acute respiratory distress, which may progress to multiorgan failure and death. Severe COVID-19 disease is accompanied by reduced erythrocyte turnover, low hemoglobin levels along with increased total bilirubin and ferritin serum concentrations. Moreover, expansion of erythroid progenitors in peripheral blood together with hypoxia, anemia, and coagulopathies highly correlates with severity and mortality. We demonstrate that SARS-CoV-2 directly infects erythroid precursor cells, impairs hemoglobin homeostasis and aggravates COVID-19 disease.

Methods

Erythroid precursor cells derived from peripheral CD34+ blood stem cells of healthy donors were infected in vitro with SARS-CoV-2 alpha variant and differentiated into red blood cells (RBCs). Hemoglobin and iron metabolism in hospitalized COVID-19 patients and controls were analyzed in plasma-depleted whole blood samples. Raman trapping spectroscopy rapidly identified diseased cells.

Results

RBC precursors express ACE2 receptor and CD147 at day 5 of differentiation, which makes them susceptible to SARS-CoV-2 infection. qPCR analysis of differentiated RBCs revealed increased HAMP mRNA expression levels, encoding for hepcidin, which inhibits iron uptake. COVID-19 patients showed impaired hemoglobin biosynthesis, enhanced formation of zinc-protoporphyrine IX, heme-CO2, and CO-hemoglobin as well as degradation of Fe-heme. Moreover, significant iron dysmetablolism with high serum ferritin and low serum iron and transferrin levels occurred, explaining disturbances of oxygen-binding capacity in severely ill COVID-19 patients.

Conclusions

Our data identify RBC precursors as a direct target of SARS-CoV-2 and suggest that SARS-CoV-2 induced dysregulation in hemoglobin- and iron-metabolism contributes to the severe systemic course of COVID-19. This opens the door for new diagnostic and therapeutic strategies.

Graphical Abstract
Neuroinflammatory Modulation of Extracellular Vesicle Biogenesis and Cargo Loading
Authors: Spiers Jereme G. , Vassileff Natasha , Hill Andrew F.
Published: February 2022

Increasing evidence suggests neuroinflammation is a highly coordinated response involving multiple cell types and utilising several different forms of cellular communication. In addition to the well documented cytokine and chemokine messengers, extracellular vesicles (EVs) have emerged as key regulators of the inflammatory response. EVs act as vectors of intercellular communication, capable of travelling between different cells and tissues to deliver selectively packaged protein, miRNA, and lipids from the parent cell. During neuroinflammation, EVs transmit specific inflammatory mediators, particularly from microglia, to promote inflammatory resolution. This mini-review will highlight the novel neuroinflammatory mechanisms contributing to the biogenesis and selective packaging of EVs.
Influence of antidepressant use on 123I-MIBG heart and lung uptakes in the diagnosis of Lewy body disease
Authors: Adaniya Shinobu , Takahashi Miwako , Koyama Keitaro , Ogane Kenichiro , Momose Toshimitsu
Published: February 2022

Objective

The clinical significance of decreased physiological lung uptake of 123I-metaiodobenzylguanidine (MIBG) has not been well investigated. This study aimed to elucidate the association between a decrease in lung MIBG uptake with antidepressant intake and the myocardial MIBG uptake in patients who were clinically diagnosed with Lewy body disease (LBD) and patients who were diagnosed as not having LBD.

Methods

We retrospectively reviewed the heart and lung uptakes on 167 consecutive MIBG scans, antidepressant status, and clinical diagnosis of LBD. The images were visually classified into two groups: decreased lung uptake and preserved lung uptake. A semi-quantitative analysis was performed using the heart-to-mediastinum ratio (H/M), lung-to-mediastinum ratio (L/M), and myocardial washout rate (WR).

Results

All 17 patients with decreased lung uptake were on treated with antidepressants, while none of the 150 patients with preserved lung uptake were treated with any antidepressants. Of the 17 patients with decreased lung uptake, 6 patients were clinically diagnosed as LBD and other 11 were clinically diagnosed as non-LBD. There was not significant difference in early H/M, delayed H/M, and myocardial WR between the 11 non-LBD patients with decreased lung uptake and 83 non-LBD patients with preserved lung uptake (2.87 ± 0.69 vs. 2.89 ± 0.44, 3.09 ± 0.48 vs. 2.98 ± 0.59, and 21.8 ± 11.3% vs. 21.1 ± 12.5%, respectively). Moreover, in LBD patients, there were no significant differences in those values between six patients with decreased lung uptake and 67 patients with preserved lung uptake (1.68 ± 0.32 vs. 1.73 ± 0.42, 1.34 ± 0.21 vs. 1.54 ± 0.57, 46.2 ± 22.8% vs. 42.8 ± 21.3%, respectively).

Conclusions

Antidepressants probably blocked MIBG uptake in the lungs, and a decreased lung uptake was not significantly associated with heart uptake. A remarkable decrease in lung uptake can be a signal to check a patient’s medication status.
Dynamic Light Scattering on Nanoparticles in Microgravity in a Drop Tower
Authors: Pyttlik Andrea , Kuttich Björn , Kraus Tobias
Published: February 2022

Gravity affects colloidal dispersions via sedimentation and convection. We used dynamic light scattering (DLS) to quantify the mobility of nanoparticles on ground and in microgravity. A DLS instrument was adapted to withstand the accelerations in a drop tower, and a liquid handling set-up was connected in order to stabilize the liquid temperature and enable rapid cooling or heating. Light scattering experiments were performed in the drop tower at ZARM (Bremen, Germany) during a microgravity interval of 9.1 s and compared to measurements on ground. Particle dynamics were analyzed at constant temperature and after a rapid temperature drop using a series of DLS measurements with 1 s integration time. We observed nanoparticles with average gold core diameters of 7.8 nm and non-polar oleylamine shells that were dispersed in tetradecane and had an average hydrodynamic diameter of 21 nm. The particles did not change their diameter in the observed temperature range. The particle dynamics inferred from DLS on ground and in microgravity were in good agreement, demonstrating the possibility to perform reliable DLS measurements in a drop tower.
Mithraism under the microscope: new revelations about rituals through micromorphology, histotaphonomy and zooarchaeology
Authors: Lo Russo Sarah , Brönnimann David , Deschler-Erb Sabine , Ebnöther Christa , Rentzel Philippe
Published: February 2022

This paper is the first to use an innovative multi-proxy approach to obtain insights into hitherto unknown Mithraic cult practices. Using soil micromorphology, zooarchaeology and histotaphonomy, we investigated two mithraea (Biesheim FR, Kempraten CH) and one cult cave (Zillis CH). This was also the first study to include micromorphological and histotaphonomic approaches within the context of Mithraic research. The systematic evaluation of thermal alteration and bioerosion (in particular bacterial attack) on the animal bones, in combination with zooarchaeological results, allows a differentiation between various (cult) practices involving animals. Thanks to the micromorphological investigations, it was possible to demonstrate that repeatedly spreading combustion residues and renewing the floor were important parts of the cult practices of Mithraism. Our results suggest that a cult practice consisting of several stages was carried out on all three sites. Our study highlights that multiple floor constructions as well as the related sediments and (micro-) artefacts of cultic indoor features are important sources of information.
Caught in travertine: computed tomography reveals the youngest record of Amphicyon giganteus from the travertine deposits of Karacalar (late middle Miocene, central Anatolia, Turkey)
Authors: van der Hoek Julien , Karabaşoğlu Aslı , Mayda Serdar , van den Hoek Ostende Lars W.
Published: February 2022

A computed tomography scan of a travertine slab from the Karacalar Silver Travertine Quarry (Afyonkarahisar Province, Turkey) revealed the presence of an encased partial cranium, partial mandible and three vertebrae. 3D reconstruction of the fossil helped identifying it asAmphicyon giganteus.As the travertine caps a section correlated to MN7/8, the specimen represents the youngest record ofAmphicyon giganteus, the known range previously being limited to MN4 – MN6. This young age is in line with the more advanced morphology of the lower molars.
Applying BERT Embeddings to Predict Legal Textual Entailment
Authors: Wehnert Sabine , Dureja Shipra , Kutty Libin , Sudhi Viju , De Luca Ernesto William
Published: February 2022

Textual entailment classification is one of the hardest tasks for the Natural Language Processing community. In particular, working on entailment with legal statutes comes with an increased difficulty, for example in terms of different abstraction levels, terminology and required domain knowledge to solve this task. In course of the COLIEE competition, we develop three approaches to classify entailment. The first approach combines Sentence-BERT embeddings with a graph neural network, while the second approach uses the domain-specific model LEGAL-BERT, further trained on the competition’s retrieval task and fine-tuned for entailment classification. The third approach involves embedding syntactic parse trees with the KERMIT encoder and using them with a BERT model. In this work, we discuss the potential of the latter technique and why of all our submissions, the LEGAL-BERT runs may have outperformed the graph-based approach.
Real-time data based thermal comfort prediction leading to temperature setpoint control
Authors: Kumar T. M. Sanjeev , Kurian Ciji Pearl
Published: February 2022

The different thermal comfort indices such as Predictive Mean Vote (PMV), Standard Effective Temperature (SET), and Thermal Sensations (TS) have been used to predict occupants’ thermal comfort in a building. The advances in the machine learning approach help overcome the challenges of predicting current traditional thermal indices in a real-time environment. The different indices have different types of data samples (continuous/labelled). Therefore, while considering the machine learning technique in developing the models of the predictive thermal indices, it is essential to select the vital features, the proper learning type, the algorithm, and the evaluation method to establish the models of the predictive thermal comfort. The main focus of this paper is on the development of the ML model and the evaluation technique that helps in selecting the best model in predicting the thermal indices. This work proposes the new neighbourhood-component-analysis Bayesian-optimization-algorithm-based artificial-neural-network to develop a predictive model for the thermal indices. Here, we have proposed a regression-based model to predict PMV, SET and a classification-based model to predict 7-point TS. The statistical-testing results specify that the ANN model's performance is highly accurate and more reliable in predicting the thermal perception in a real-time environment. The performance of the selected model is validated using subjective measures. This prediction leads to the pre-emptive control of the setpoint temperature of the air-conditioning unit, hence resulting in energy efficiency and comfort.
Circle of Willis Morphology in Primary Intracerebral Hemorrhage
Authors: Xu Mangmang , Wu Qian , Cheng Yajun , Zhang Shuting , Tao Wendan , Zhang Shihong , Wang Deren , Liu Ming , Wu Bo
Published: February 2022

We aimed to study the distribution of Circle of Willis (CoW) morphology and its association with intracerebral hemorrhage (ICH) etiology and cerebral small vessel disease (CSVD) burden. Patients with primary ICH who had brain MRIs were consecutively enrolled between March 2012 and January 2021. CoW morphology, CSVD features and the combined CSVD burden (including global CSVD burden, total hypertensive arteriopathy [HA] burden, and total cerebral amyloid angiopathy [CAA] burden) were assessed. CoW morphology included poor CoW (defined as CoW score 0–2), incomplete CoW, and complete fetal-variant of the posterior communicating artery (CFPcoA). Among 296 patients enrolled, 215 were included in the analysis. There was no significant difference among HA-, CAA-, and mixed-ICH in each CoW morphology. Exploratory subgroup analyses suggested that poor CoW was associated with a greater incidence of HA-ICH and low incidence of mixed ICH in patients aged < 60 years, while mixed ICH occurred more frequently in patients with CFPcoA, especially in those without hypertension history (all p < 0.050). Additionally, incomplete CoW was correlated with a larger incidence of lacunes (adjusted OR [adOR] 2.114, 95% CI 1.062–4.207), microbleeds ≥ 5 (adOR 2.437, 95% CI 1.187–5.002), and therefore the combined CSVD burden (adOR 1.194, 95% CI 1.004–1.419 for global CSVD burden, adOR 1.343, 95% CI 1.056–1.707 for total CAA burden), independent of modifiable vascular risk factors, but not age and sex. The CoW might therefore have a potential impact on ICH etiology and is associated with a greater CSVD burden. Our findings are novel, and need to be verified in future studies.
Sexual Pleasure Matters (Especially for Women) — Data from the German Sexuality and Health Survey (GeSiD)
Authors: Klein Verena , Laan Ellen , Brunner Franziska , Briken Peer
Published: February 2022

Introduction

Sexual pleasure has been a neglected issue in sexual health policies. Emerging trends in public health, however, emphasize the importance of sexual pleasure in preventing negative sexual health outcomes.

Methods

Using data from the German Sexuality and Health Survey (GeSiD), we tested the assumption that sexual pleasure is associated with sexual health, including a special focus on the role of gender. Participants were interviewed about their sexual experiences and health between October 2018 and September 2019. The analytical sample included 3472 partnered and single women and men who had been sexually active with a partner in the past 12 months. We examined if sexual pleasure was associated with various sexual health indicators (i.e., communication about sexually transmitted infections (STIs), condom use, and absence of sexual problems).

Results

Women reported less sexual pleasure than men. Results further indicate that sexual pleasure was associated with more sexual health indicators in women than in men.

Conclusions

Supporting emerging trends in public health our results emphasize the importance of sexual pleasure in preventing negative sexual health outcomes.

Policy Implications.

To promote (especially women’s) sexual health, our results call for the implementation of comprehensive sexuality education programs that focus on more positive aspects of sex, such as sexual pleasure and agency.
Development of a novel rapamycin loaded nano- into micro-formulation for treatment of lung inflammation
Authors: Craparo Emanuela Fabiola , Drago Salvatore Emanuele , Quaglia Fabiana , Ungaro Francesca , Cavallaro Gennara
Published: February 2022

It has recently emerged that drugs such as the mTOR inhibitor rapamycin (Rapa) may play a key role in the treatment of airway inflammation associated with lung diseases, such as chronic obstructive pulmonary disease, asthma, and cystic fibrosis. Nevertheless, Rapa clinical application is still prevented by its unfavorable chemical-physical properties, limited oral bioavailability, and adverse effects related to non-specific biodistribution. In this paper, the design and production of a novel formulation of Rapa based on nano into micro (NiM) particles are detailed. To achieve it, Rapa-loaded nanoparticles were produced by nanoprecipitation of an amphiphilic pegylated poly-ɛ-caprolactone/polyhydroxyethyl aspartamide graft copolymer. The obtained nanoparticles that showed a drug loading of 14.4 wt% (corresponding to an encapsulation efficiency of 82 wt%) did not interact with mucins and were able to release and protect Rapa from degradation in simulated lung and cell fluids. To allow their local administration to the lungs as a dry powder, particle engineering at micro-sized level was done by embedding nanoparticles into mannitol-based microparticles by spray drying. Obtained NiM particles had a mean diameter of about 2-µ, spherical shape and had good potential to be delivered to the lungs by a breath-activated dry powder inhalers. Rheological and turbidity experiments showed that these NiM particles can dissolve in lung simulated fluid and deliver the Rapa-loaded pegylated nanoparticles, which can diffuse through the mucus layer.

Graphical abstract