bluuebunny/crossref_metadata_2025 huggingface dataset:
Diverticulitis Is Associated with Increased Risk of Colon Cancer—A Nationwide Register-Based Cohort Study
Authors: Mortensen Laura Quitzau , Andresen Kristoffer , Thygesen Lau , Pommergaard Hans-Christian , Rosenberg Jacob
Published: April 2024

Background: An association between diverticulitis and colon cancer has been proposed. The evidence is conflicting, and the guidelines differ regarding recommended follow-up with colonoscopy after an episode of diverticulitis. To guide regimes for follow-up, this study aimed to investigate if patients with diverticulitis have an increased risk of colon cancer. Methods: This study is reported according to the RECORD statement. We performed a cohort study with linked data from nationwide Danish registers. The inclusion period was 1997–2009, and the complete study period was 1995–2013. The primary outcome was the risk of developing colon cancer estimated using a Cox regression analysis with time-varying covariates. We performed a sensitivity analysis on a cohort of people with prior colonoscopies, comparing the risk of colon cancer between the diverticulitis group and the control group. Results: We included 29,173 adult males and females with diverticulitis and 145,865 controls matched for sex and age. The incidence proportion of colon cancer was 2.1% (95% confidence interval (CI) 1.9–2.3) in the diverticulitis group and 1.5% (95% CI 1.4–1.5) in the matched control group (hazard ratio 1.6; 95% CI 1.5–1.8). The risk of having a colon cancer diagnosis was significantly increased in the first six months after inclusion (hazard ratio 1.7; 95% CI 1.5–1.8), and hereafter there was a lower risk in the diverticulitis group compared with controls (hazard ratio 0.8; 95% CI 0.7–0.9). This protective effect lasted eight years. The increased risk of colon cancer during the first six months after diverticulitis was also found in the cohort with prior colonoscopies. Conclusions: The risk of a colon cancer diagnosis was significantly increased for patients with diverticulitis 0–6 months after the diagnosis of diverticulitis. Hereafter, we found a protective effect of diverticulitis until eight years later, possibly due to a screening effect. We recommend a follow-up colonoscopy after the first diagnosis of diverticulitis.
Numerical Investigation of Butterfly Valve Performance in Variable Valve Sizes, Positions and Flow Regimes
Authors: Bairagi Anutam , He Mingfu , Chen Minghui
Published: April 2024

Reliability and efficiency of valves are necessary for precise control and sufficient heat-flow to heat application plants for the integrated energy systems of nuclear power plants (NPPs). Strategic Management Analysis Requirement and Technology (SMART) valves’ ability to control flow and assess environmental parameters stands out for these requirements. Their ability to sustain the downstream flow rate, prevent reverse flow, and maintain pressure in the heat transport loop is much more efficient with the integration of sensors and intelligent algorithms. For assessing valve performance and monitoring, mechanical design and operating conditions are two important parameters. In this study, the butterfly valves of three different sizes are simulated with water and steam using STAR-CCM+ in various flow regimes and positions to analyze performance parameters to strategize an automated control system for efficiently balancing the heat–transport network. Also, flow behavior is studied using velocity and pressure fields for valve–body geometry optimization. It can be observed, through performance parameters, that the valves are suitable for operation between 30° and 90° positions with significantly low loss coefficients and high flow coefficients, and the performance parameters follow a certain pattern in both water and steam flow in each scenario.
Prevalence and Species Distribution of Candida Clinical Isolates in a Tertiary Care Hospital in Ecuador Tested from January 2019 to February 2020
Authors: Acosta-Mosquera Yessenia , Tapia Juan Carlos , Armas-González Rubén , Cáceres-Valdiviezo María José , Fernández-Cadena Juan Carlos , Andrade-Molina Derly
Published: April 2024

The incidence of candidemia in healthcare centers is associated with high morbidity and mortality. Frequency varies significantly among regions, with some species being more prevalent than others in Latin America. In this study, 191 clinical Candida isolates were collected from a major hospital in Ecuador from January 2019 to February 2020 aiming to assess their prevalence and distribution. After data processing, 168 isolates characterized by the VITEK 2 system were subsequently identified by ITS sequencing. Results showed diverse Candida species distributions, with C. albicans and C. tropicalis being the most prevalent across different clinical sources. In hospitalized individuals, C. tropicalis (38%) and C. albicans (37%) were the most prevalent, followed by, C. parapsilosis (16%), C. glabrata (5%), and other non-Candida albicans (NCA) species (6%). Conversely, C. parapsilosis (48%), C. albicans (20%), and C. glabrata (14%), associated with candidemia, were the most common in blood and CSF. Additionally, uncommon NCA species such as C. haemulonii, C. kefyr, and C. pelliculosa were identified in Ecuador for the first time. Discrepancies in species identification were observed between the VITEK 2 system and ITS sequencing, coinciding at 85%. This highlights the need for ongoing surveillance and identification efforts in Ecuador’s clinical and epidemiological settings.
Impact of Nitric Oxide-Release Kinetics on Antifungal Activity
Authors: Grayton Quincy E. , Conlon Ivie L. , Broberg Christopher A. , Schoenfisch Mark H.
Published: April 2024

Pathogenic fungi are an increasing health threat due to the rise in drug resistance. The limited number of antifungals currently available and growing incidence of multi-drug-resistant fungi has caused rising healthcare costs and a decreased quality of life for patients with fungal infections. Nitric oxide (NO) has previously been shown to act as an antimicrobial agent, albeit with a limited understanding of the effects of the NO-release kinetics against pathogenic fungi. Herein, the antifungal effects of four nitric oxide-releasing small molecules were studied against the pathogenic fungi Candida albicans, Candida auris, Cryptococcus neoformans, and Aspergillus fumigatus, to demonstrate the broad-spectrum antifungal activity of NO. A bolus dose of NO was found to eradicate fungi after 24 h, where nitric oxide donors with shorter half-lives achieved antifungal activity at lower concentrations and thus had wider selectivity indexes. Each NO donor was found to cause a severe surface destruction of fungi, and all NO donors exhibited compatibility with currently prescribed antifungals against several different fungi species.
Age-Related Conservation in Plant–Soil Feedback Accompanied by Ectomycorrhizal Domination in Temperate Forests in Northeast China
Authors: Bai Zhen , Ye Ji , Liu Shu-Fang , Sun Hai-Hong , Yuan Zuo-Qiang , Mao Zi-Kun , Fang Shuai , Long Shao-Fen , Wang Xu-Gao
Published: April 2024

This study investigates the effects of forest aging on ectomycorrhizal (EcM) fungal community and foraging behavior and their interactions with plant–soil attributes. We explored EcM fungal communities and hyphal exploration types via rDNA sequencing and investigated their associations with plant–soil traits by comparing younger (~120 years) and older (~250 years) temperate forest stands in Northeast China. The results revealed increases in the EcM fungal richness and abundance with forest aging, paralleled by plant–soil feedback shifting from explorative to conservative nutrient use strategies. In the younger stands, Tomentella species were prevalent and showed positive correlations with nutrient availability in both the soil and leaves, alongside rapid increases in woody productivity. However, the older stands were marked by the dominance of the genera Inocybe, Hymenogaster, and Otidea which were significantly and positively correlated with soil nutrient contents and plant structural attributes such as the community-weighted mean height and standing biomass. Notably, the ratios of longer-to-shorter distance EcM fungal exploration types tended to decrease along with forest aging. Our findings underscore the integral role of EcM fungi in the aging processes of temperate forests, highlighting the EcM symbiont-mediated mechanisms adapting to nutrient scarcity and promoting sustainability in plant–soil consortia.
Sustaining Family Businesses through Business Incubation: An Africa-Focused Review
Authors: Iwu Chux Gervase , Malawu Nobandla , Ndlovu Elona Nobukhosi , Makwara Tendai , Sibanda Lucky
Published: April 2024

The influence of business incubation systems on family businesses in African economies has not been thoroughly investigated despite the potential contribution of family businesses to Africa’s economic expansion and the attainment of development goals outlined in the Africa Development Agenda 2063 and the Sustainable Development Goals. Therefore, this study investigates the potential benefits that family businesses in Africa can derive from engaging in business incubation. This study utilised an integrative literature review methodology to investigate the research question. Twenty-three peer-reviewed articles were systematically selected from the Scopus, Web of Science, and Google Scholar databases using the following combination of phrases: “family business” and either “business incubation” or “business incubator”. The findings suggest ways to create a mutually beneficial relationship between family businesses and business incubators to improve long-term sustainability, promote collaboration, facilitate knowledge transfer, and foster an entrepreneurial ecosystem. It also recognises challenges, such as cultural alignment in family businesses. Business incubators in Africa can improve the sustainability of family businesses, such as during the succession, by offering support, resources, and guidance. The South African experience is a role model for the rest of the continent, in this regard. Future research should broaden the sources beyond the three databases utilised, including non-peer-reviewed sources such as grey literature, and extend the focus beyond developing economies.
The Temporal and Spatial Characteristics of Ecological Security Pattern in the Loess Plateau, China
Authors: Luo Manya , Jia Xia , Zhao Yonghua , Wang Huanyuan , Chen Chunyang , Li Dongqian , Yang Shuyuan , Li Juan
Published: April 2024

As a typical ecologically fragile area, the ecological security of the Loess Plateau has been seriously threatened. Ecological security patterns (ESP) have gradually become an effective method for protecting ecological security and supporting the management and sustainable development of ecosystems. Therefore, this study constructed a novel ESP based on ecological “function–structure”, utilizing minimum cumulative resistance (MCR) to identify ecological source areas and corridors. Additionally, time scales were introduced into the ESP, allowing for a comprehensive analysis of the spatiotemporal heterogeneity of ecological security in the Loess Plateau. The study revealed that the number of ecological sources decreased from southeast to northwest, with 27, 41, and 77 sources covering total areas of 4263.810 km2, 18,566.034 km2, and 113,209.595 km2 from 2000 to 2020, respectively. Similarly, the number and complexity of ecological corridors increased over the same time period, with 64, 85, and 105 corridors totaling lengths of 4579.326 km, 6526.996 km, and 7015.174 km, respectively. The expansion of ecological security zones was mainly observed in the southeast part of the Loess Plateau. Overall, the ESP of the Loess Plateau saw an improvement, with the southeastern part showing better ecological security than the northwestern part. These findings hold great significance for regional ecological security evaluations and are crucial for promoting ecological management and healthy development in the Loess Plateau.
Visual Cues to Speakers’ Religious Affiliation and Listeners’ Understanding of Second Language French Speech
Authors: Kennedy Sara , Trofimovich Pavel , Lindberg Rachael , Tekin Oguzhan
Published: April 2024

Previous research has shown that speakers’ visual appearance influences listeners’ perception of second language (L2) speech. In Québec, Canada, the context of this study, pandemic mask mandates and a provincial secularism law elicited strong societal reactions. We therefore examined how images of speakers wearing religious and nonreligious coverings such as medical masks and headscarves influenced the comprehensibility (listeners’ ease of understanding) and intelligibility of L2 French speech. Four L2 French women from first language (L1) Arabic backgrounds wore surgical masks while recording 40 sentences from a standardized French-language speech perception test. A total of 104 L1 French listeners transcribed and rated the comprehensibility of the sentences, paired with images of women in four visual conditions: uncovered face, medical mask, hijab (headscarf), and niqab (religious face covering). Listeners also completed a questionnaire on attitudes toward immigrants, cultural values, and secularism. Although intelligibility was high, sentences in the medical mask condition were significantly more intelligible and more comprehensible than those in the niqab condition. Several attitudinal measures showed weak correlations with intelligibility or comprehensibility in several visual conditions. The results suggest that listeners’ understanding of L2 sentences was negatively affected by images showing speakers’ religious affiliation, but more extensive follow-up studies are recommended.
Radio-Lect: Spanish/English Code-Switching in On-Air Advertisements
Authors: Derrick Roshawnda A.
Published: April 2024

The 2020 census reports that 61.2 million Latinxs live in the US, totaling around 19% of all residents, forming the country’s largest minority population. With the growing number of Latinxs, there has been a higher level of contact between Spanish and English leading to language mixing or code-switching (CS) in mainstream American culture. This paper examines the Spanish/English CS in radio advertisements on Los Angeles’s 96.3 La Mega, a bilingual radio station geared towards today’s youth. Using Derrick’ 2015 sentential framework for the linguistic analysis of multilingual sentences, I carry out a sentence-by-sentence analysis of the linguistic nature of the on-air bilingual advertisements. I explore both national advertisements, as well as DJ-endorsed advertisements, to discern whether they follow the patterns previously pointed out in the literature for positive consumer evaluations of Spanish/English bilingual advertisements. Furthermore, I am interested in if these advertisements are in line with the ethos of 96.3 La Mega, which prides itself on being fully bilingual. This research will shed light on the linguistic nature of contemporary strategies being used in bilingual advertisements for the US Latinx community and marketing tactics designed to encourage their consumerism.
Optimal Design of Boundary Angle for Gas Foil Thrust Bearing Thermal Performance
Authors: Hu Bin , Hou Anping , Deng Rui , Yang Xiaodong , Wu Zhiyong , Ni Qifeng , Li Zhong
Published: April 2024

As the energy density and efficiency requirements of air compressors continue to increase, gas foil thrust bearings face a high risk of thermal failure due to their elevated speed and limited cooling space. This paper proposes a novel structure for gas foil thrust bearings with enhanced thermal characteristics. A thermo-elastic–hydrodynamic model is developed using a thermal-fluid–solid interaction approach to investigate aerodynamic and thermal performance. The load capacity and thermal characteristics of nine different boundary angles are analyzed. The model is validated, and the actual characteristics of gas foil bearings with various boundary angles are examined using a test rig. The results indicate that, compared to conventional gas foil thrust bearings with a boundary angle of 0°, the new structure with a boundary angle ranging from −10° to −5° not only maintains the load carrying capacity but also improves thermal characteristics. Furthermore, this improvement becomes more pronounced with higher rotational speeds. Therefore, the proposed optimization is advantageous in reducing the risk of thermal failure.
5,5′-Thiobis(3-bromoisothiazole-4-carbonitrile)
Authors: Kalogirou Andreas S. , Koutentis Panayiotis A.
Published: April 2024

The reaction of sodium 2,2-dicyanoethene-1,1-bis(thiolate) with bromine (2 equiv.) in CCl4 gave 3,5-dibromoisothiazole-3-carbonitrile and 5,5′-thiobis(3-bromoisothiazole-4-carbonitrile) in 7% and 18% yields, respectively. The latter novel compound was fully characterized.
Research of Energy Efficiency and Environmental Performance of Vehicle Power Plant Converted to Work on Alternative Fuels
Authors: Mateichyk Vasyl , Kryshtopa Sviatoslav , Kryshtopa Liudmyla , Smieszek Miroslaw , Kostian Nataliia , Mosciszewski Jakub , Marchuk Nazar
Published: April 2024

The use of alternative fuels remains an important factor in solving the problem of reducing harmful substances caused by vehicles and decarbonising transport. It is also important to ensure the energy efficiency of vehicle power plants when using different fuels at a sufficient level. The article presents the results of theoretical and experimental studies of the conversion of diesel engine to alternative fuels with hydrogen admixtures. Methanol is considered as an alternative fuel which is a cheaper alternative to commercial diesel fuel. The chemical essence of improving the calorific value of alternative methanol fuel was investigated. Studies showed that the energy effect of burning an alternative mixture with hydrogen additives exceeds the effect of burning the same amount of methanol fuel. The increase in combustion energy and engine power is achieved as a result of heat from efficient use of the engine exhaust gases and chemical conversion of methanol. An experimental installation was created to study the work of a converted diesel engine on hydrogen–methanol mixtures and thermochemical regeneration processes. Experimental studies of the energy and environmental parameters of diesel engine converted to work on an alternative fuel with hydrogen admixtures have shown that engine power increases by 10–14% and emissions of harmful substances decrease.
MDER-Net: A Multi-Scale Detail-Enhanced Reverse Attention Network for Semantic Segmentation of Bladder Tumors in Cystoscopy Images
Authors: Nie Chao , Xu Chao , Li Zhengping
Published: April 2024

White light cystoscopy is the gold standard for the diagnosis of bladder cancer. Automatic and accurate tumor detection is essential to improve the surgical resection of bladder cancer and reduce tumor recurrence. At present, Transformer-based medical image segmentation algorithms face challenges in restoring fine-grained detail information and local boundary information of features and have limited adaptability to multi-scale features of lesions. To address these issues, we propose a new multi-scale detail-enhanced reverse attention network, MDER-Net, for accurate and robust bladder tumor segmentation. Firstly, we propose a new multi-scale efficient channel attention module (MECA) to process four different levels of features extracted by the PVT v2 encoder to adapt to the multi-scale changes in bladder tumors; secondly, we use the dense aggregation module (DA) to aggregate multi-scale advanced semantic feature information; then, the similarity aggregation module (SAM) is used to fuse multi-scale high-level and low-level features, complementing each other in position and detail information; finally, we propose a new detail-enhanced reverse attention module (DERA) to capture non-salient boundary features and gradually explore supplementing tumor boundary feature information and fine-grained detail information; in addition, we propose a new efficient channel space attention module (ECSA) that enhances local context and improves segmentation performance by suppressing redundant information in low-level features. Extensive experiments on the bladder tumor dataset BtAMU, established in this article, and five publicly available polyp datasets show that MDER-Net outperforms eight state-of-the-art (SOTA) methods in terms of effectiveness, robustness, and generalization ability.
Numerical Investigation of Supersonic Flow over a Wedge by Solving 2D Euler Equations Utilizing the Steger–Warming Flux Vector Splitting (FVS) Scheme
Authors: Wolff Mitch , Abada Hashim H. , Saad Hussein Awad Kurdi
Published: April 2024

Supersonic flow over a half-angle wedge (θ = 15°) with an upstream Mach number of 2.0 was investigated using 2D Euler equations where sea level conditions were considered. The investigation employed the Steger–Warming flux vector splitting (FVS) method executed in MATLAB 9.13.0 (R2022b) software. The study involved a meticulous comparison between theoretical calculations and numerical results. Particularly, the research emphasized the angle of oblique shock and downstream flow properties. A substantial iteration count of 2000 iteratively refined the outcomes, underscoring the role of advanced computational resources. Validation and comparative assessment were conducted to elucidate the superiority of the Steger–Warming flux vector splitting (FVS) scheme over existing methodologies. This research serves as a link between theoretical rigor and practical applications in high-speed aerospace design, enhancing the efficiency of aircraft components.
Inter-Channel Correlation Modeling and Improved Skewed Histogram Shifting for Reversible Data Hiding in Color Images
Authors: He Dan , Cai Zhanchuan , Zhou Dujuan , Chen Zhihui
Published: April 2024

Reversible data hiding (RDH) is an advanced data protection technology that allows the embedding of additional information into an original digital medium while maintaining its integrity. Color images are typical carriers for information because of their rich data content, making them suitable for data embedding. Compared to grayscale images, color images with their three color channels (RGB) enhance data embedding capabilities while increasing algorithmic complexity. When implementing RDH in color images, researchers often exploit the inter-channel correlation to enhance embedding efficiency and minimize the impact on image visual quality. This paper proposes a novel RDH method for color images based on inter-channel correlation modeling and improved skewed histogram shifting. Initially, we construct an inter-channel correlation model based on the relationship among the RGB channels. Subsequently, an extended method for calculating the local complexity of pixels is proposed. Then, we adaptively select the pixel prediction context and design three types of extreme predictors. The improved skewed histogram shifting method is utilized for data embedding and extraction. Finally, experiments conducted on the USC-SIPI and Kodak datasets validate the superiority of our proposed method in terms of image fidelity.
Asymptotic Behavior of Stochastic Reaction–Diffusion Equations
Authors: Wen Hao , Wang Yuanjing , Liu Guangyuan , Liu Dawei
Published: April 2024

In this paper, we concentrate on the propagation dynamics of stochastic reaction–diffusion equations, including the existence of travelling wave solution and asymptotic wave speed. Based on the stochastic Feynman–Kac formula and comparison principle, the boundedness of the solution of stochastic reaction–diffusion equations can be obtained so that we can construct a sup-solution and a sub-solution to estimate the upper bound and the lower bound of wave speed.
Spatial Network Analysis of Coupling Coordination between Digital Financial Inclusion and Common Prosperity in the Yangtze River Delta Urban Agglomeration
Authors: Zeng Fanlong , Sun Huaping
Published: April 2024

Digital financial inclusion and common prosperity are pivotal elements in promoting the sustainable socioeconomic development of China. This study introduces a novel Multi-Criteria Decision Analysis (MCDA) method to evaluate the Common Prosperity Index (CPI). Using this index, alongside the Digital Financial Inclusion Index (DFII) released by Peking University, it examines the evolution of the coupling coordination relationship between digital financial inclusion and common prosperity within the Yangtze River Delta (YRD) urban agglomeration from 2011 to 2021. By integrating gravity models and social network analysis, in this paper, we thoroughly investigate the spatiotemporal evolution characteristics of the spatial network of this coupling coordination relationship. The results indicate that both the DFII and CPI generally exhibit an upward trend, but the decline in the coupling degree reflects a weakened interaction strength between them. Specifically, Anhui significantly lags behind Jiangsu, Zhejiang, and Shanghai in the development of digital financial inclusion and common prosperity, indicating regional development imbalances. Furthermore, the strength of spatial connections in city coupling coordination has significantly increased, with Nanjing’s siphon effect on cities in Anhui becoming markedly stronger, and the number of core cities in the network increasing, which demonstrates a geographical proximity feature in network development. Additionally, the overall network characteristics are transitioning towards higher density and “small-world” properties, suggesting a trend toward network stabilization. The disparity in centrality among cities has decreased, with an overall enhancement in centrality, where the spatial spillover effects from core areas such as Hangzhou-Ningbo, Nanjing-Changzhou, and Shanghai-Suzhou-Wuxi significantly promote the development of peripheral cities. Based on these findings, this paper proposes policy recommendations for the sustainable development of digital financial inclusion and common prosperity in the YRD region.
A Weighted Skew-Logistic Distribution with Applications to Environmental Data
Authors: Cortés Isaac , Reyes Jimmy , Iriarte Yuri A.
Published: April 2024

Skewness and bimodality properties are frequently observed when analyzing environmental data such as wind speeds, precipitation levels, and ambient temperatures. As an alternative to modeling data exhibiting these properties, we propose a flexible extension of the skew-logistic distribution. The proposal corresponds to a weighted version of the skewed logistic distribution, defined by a parametric weight function that allows shapes with up to three modes for the resulting density. Parameter estimation via the maximum likelihood approach is discussed. Simulation experiments are carried out to evaluate the performance of the estimators. Applications to environmental data illustrating the utility of the proposal are presented.
Local C0,1-Regularity for the Parabolic p-Laplacian Equation on the Group SU(3)
Authors: He Yongming , Yu Chengwei , Wang Hongqing
Published: April 2024

In this article, when 2≤p≤4, we establish the Cloc0,1-regularity of weak solutions to the degenerate parabolic p-Laplacian equation ∂tu=−∑i=16Xi*(|∇Hu|p−2Xiu) on the group SU(3) granted with horizontal vector fields X1, …, X6. Compared to the Heisenberg group, Hn, we obtained the optimal range of p; that is, 2≤p≤4.
Investigation of Optimal Needle Position for Radiofrequency Ablation-Based Blockade of Interspace between the Popliteal Artery and the Posterior Capsule of the Knee: A Cadaveric Study
Authors: Kim Jiyoung , Kim Sang Hyun , Shin Hwa Yong , Kim In-Beom , Kim Bae Wook , Lee U-Young , Park Hue Jung
Published: April 2024

Background and Objectives: The interspace between the popliteal artery and the posterior capsule of the knee (iPACK) block has been widely used in perioperative settings to control posterior knee pain and can additionally be used for chronic knee pain. In this cadaveric study, we aimed to investigate the needle tip position and its proximity to the articular branch of the tibial nerve (ABTN) during an iPACK-targeted radiofrequency procedure. Materials and Methods: An ultrasound-guided iPACK block was performed on 20 knees of 10 cadavers. We injected 0.1 mL each of blue and green gelatinous dye near the tibial artery (point A) and posterior knee capsule (point B), respectively, and evaluated the spread of both around the ABTN. For a hypothetical conventional radiofrequency ablation (RFA) lesion (diameter, 2.95 mm) and cooled RFA lesion (diameter, 4.9 mm), we counted the number of specimens in which the ABTNs would be captured. Results: The percentage of specimens in which the ABTN would be captured by a cooled RFA lesion was 64.71% at point A and 43.75% at point B (p = 0.334). Meanwhile, the percentage of specimens in which the ABTN would be captured by a conventional RFA lesion was 58.82% from point A and 25% from point B (p = 0.065). Conclusions: When performing an RFA-based iPACK block, the needle tip may be positioned either lateral to the tibial artery or in the space between the posterior knee capsule and the tibial artery. However, more studies with larger samples are needed to verify these results before the clinical use of this procedure can be recommended.
The Clinical Applications of Left Atrial Strain: A Comprehensive Review
Authors: O’Neill Thomas , Kang Puneet , Hagendorff Andreas , Tayal Bhupendar
Published: April 2024

Left atrial (LA) strain imaging, which measures the deformation of the LA using speckle-tracing echocardiography (STE), has emerged recently as an exciting tool to help provide diagnostic and prognostic information for patients with a broad range of cardiovascular (CV) pathologies. Perhaps due to the LA’s relatively thin-walled architecture compared with the more muscular structure of the left ventricle (LV), functional changes in the left atrium often precede changes in the LV, making LA strain (LAS) an earlier marker for underlying pathology than many conventional echocardiographic parameters. LAS imaging is typically divided into three phases according to the stage of the cardiac cycle: reservoir strain, which is characterized by LA filling during systole; conduit strain, which describes LA deformation during passive LV filling; and booster strain, which provides information on the LA atrium during LA systole in late ventricular diastole. While additional large-population studies are still needed to further solidify the role of LAS in routine clinical practice, this review will discuss the current evidence of its use in different pathologies and explore the possibilities of its applications in the future.
Comparative Genomics Reveals Genetic Diversity and Variation in Metabolic Traits in Fructilactobacillus sanfranciscensis Strains
Authors: He Xiaxia , Yu Yujuan , Kemperman Rober , Jimenez Luciana , Ahmed Sadiq Faizan , Zhang Guohua
Published: April 2024

Fructilactobacillus sanfranciscensis is a significant and dominant bacterial species of sourdough microbiota from ecological and functional perspectives. Despite the remarkable prevalence of different strains of this species in sourdoughs worldwide, the drivers behind the genetic diversity of this species needed to be clarified. In this research, 14 F. sanfranciscensis strains were isolated from sourdough samples to evaluate the genetic diversity and variation in metabolic traits. These 14 and 31 other strains (obtained from the NCBI database) genomes were compared. The values for genome size and GC content, on average, turned out to 1.31 Mbp and 34.25%, respectively. In 45 F. sanfranciscensis strains, there were 162 core genes and 0 to 51 unique genes present in each strain. The primary functions of core genes were related to nucleotide, lipid transport, and amino acid, as well as carbohydrate metabolism. The size of core genes accounted for 41.18% of the pan-genome size in 14 F. sanfranciscensis strains, i.e., 0.70 Mbp of 1.70 Mbp. There were genetic variations among the 14 strains involved in carbohydrate utilization and antibiotic resistance. Moreover, exopolysaccharides biosynthesis-related genes were annotated, including epsABD, wxz, wzy. The Type IIA & IE CRISPR-Cas systems, pediocin PA-1 and Lacticin_3147_A1 bacteriocins operons were also discovered in F. sanfranciscensis. These findings can help to select desirable F. sanfranciscensis strains to develop standardized starter culture for sourdough fermentation, and expect to provide traditional fermented pasta with a higher quality and nutritional value for the consumers.
Evidence for Immunity against Tetanus, Diphtheria, and Pertussis through Natural Infection or Vaccination in Adult Solid Organ Transplant Recipients: A Systematic Review
Authors: Lenzing Emil , Harboe Zitta Barrella , Sørensen Søren Schwartz , Rasmussen Allan , Nielsen Susanne Dam , Rezahosseini Omid
Published: April 2024

(1) Background: We aim to systematically review the current evidence on immunity against tetanus, diphtheria, and pertussis in adult solid organ transplantation (SOT) recipients, either through natural infection or vaccination. (2) Methods: This systematic review was conducted per PRISMA guidelines. We assessed the risk of bias using the Cochrane RoB 2 and ROBINS-I and summarized the findings narratively due to the heterogeneity of the studies. (3) Results: Of the 315 screened articles, 11 were included. Tetanus immunity varied between 55% and 86%, diphtheria immunity from 23% to 75%, and pertussis immunity was between 46% and 82%. Post-vaccination immunity showed variation across the studies, with some indicating reductions and others no change, with antibody responses influenced by transplanted organs, gender, age, and immunosuppressive regimens. The single randomized study exhibited a low risk of bias, while of the ten non-randomized studies, six showed moderate and four serious risks of bias, necessitating cautious interpretation of results. (4) Conclusions: SOT recipients exhibit considerable immunity against tetanus and diphtheria at transplantation, but this immunity decreases over time. Although vaccination can enhance this immunity, the response may be suboptimal, and the increased antibody levels may not persist, underscoring the need for tailored vaccination strategies in this vulnerable population.
Evaluation of the Increased Genetic Resolution and Utility for Source Tracking of a Recently Developed Method for Genotyping Cyclospora cayetanensis
Authors: Leonard Susan R. , Mammel Mark K. , Almeria Sonia , Gebru Solomon T. , Jacobson David K. , Peterson Anna C. , Barratt Joel L. N. , Musser Steven M.
Published: April 2024

Cyclospora cayetanensis is a foodborne parasite that causes cyclosporiasis, an enteric illness in humans. Genotyping methods are used to genetically discriminate between specimens from cyclosporiasis cases and can complement source attribution investigations if the method is sufficiently sensitive for application to food items. A very sensitive targeted amplicon sequencing (TAS) assay for genotyping C. cayetanensis encompassing 52 loci was recently designed. In this study, we analyzed 66 genetically diverse clinical specimens to assess the change in phylogenetic resolution between the TAS assay and a currently employed eight-marker scheme. Of the 52 markers, ≥50 were successfully haplotyped for all specimens, and these results were used to generate a hierarchical cluster dendrogram. Using a previously described statistical approach to dissect hierarchical trees, the 66 specimens resolved into 24 and 27 distinct genetic clusters for the TAS and an 8-loci scheme, respectively. Although the specimen composition of 15 clusters was identical, there were substantial differences between the two dendrograms, highlighting the importance of both inclusion of additional genome coverage and choice of loci to target for genotyping. To evaluate the ability to genetically link contaminated food samples with clinical specimens, C. cayetanensis was genotyped from DNA extracted from raspberries inoculated with fecal specimens. The contaminated raspberry samples were assigned to clusters with the corresponding clinical specimen, demonstrating the utility of the TAS assay for traceback efforts.
Prosthetic Joint Infections Caused by Mycobacterium tuberculosis Complex—An ESGIAI–ESGMYC Multicenter, Retrospective Study and Literature Review
Authors: Auñon Alvaro , Salar-Vidal Llanos , Mahillo-Fernandez Ignacio , Almeida Francisco , Pereira Pedro , Lora-Tamayo Jaime , Ferry Tristan , Souèges Sarah , Dinh Aurélien , Escudero Rosa , Menéndez Fernández-Miranda Candela , Rico Alicia , Rossi Nicolo , Esteban Jaime
Published: April 2024

Purpose: While tuberculosis remains a significant global health concern, prosthetic joint infections (PJIs) caused by members of the Mycobacterium tuberculosis complex are exceptionally rare. Our objective is to perform a retrospective search of new cases of this disease and analyze all cases available in the literature of tuberculous PJIs, aiming to detect factors that may influence patient outcomes. Methods: The ESGIAI and ESGMYC study groups were used to collect information on non-published cases of tuberculous prosthetic joint infections (PJIs). Additionally, a literature review of all published cases of tuberculous PJIs was conducted. All identified cases in the retrospective study and in the literature review were merged and included in the statistical analysis, involving both univariate and multivariate analyses. Results: Fifteen previously unreported cases of tuberculous prosthetic joint infections (PJIs) from four countries were detailed. Among them, ten patients were female, with a median age of 76 years. The hip was affected in 13 cases. Seven patients experienced co-infection with another microorganism. Treatment approaches varied, with 13 patients undergoing implant removal, one treated with DAIR (debridement, antibiotics, and implant retention), and one case was treated with an unknown treatment method. All patients received antibiotic therapy and achieved a cure. The literature review that was conducted detected 155 published cases. Univariate analysis revealed a statistical significance for previous tuberculosis, joint, and no importance of surgery for cure. Conclusions: Tuberculous prosthetic joint infection (PJI) is a rare condition, typically presenting as a localized chronic infection. Antibiotic treatment is essential for the management of these patients, but neither surgical treatment nor duration of treatment seems to have importance in the outcome.
Type I Cystatin Derived from Cysticercus pisiformis—Stefins, Suppresses LPS-Mediated Inflammatory Response in RAW264.7 Cells
Authors: Yang Qianqian , Li Jia , Zhang Lilan , Zhao Ningning , Sun Xiaolin , Wang Zexiang
Published: April 2024

Cysticercus pisiformis is a kind of tapeworm larvae of Taenia pisiformis, which parasitizes the liver envelope, omentum, mesentery, and rectum of rodents such as rabbits. Cysteine protease inhibitors derived from helminth were immunoregulatory molecules of intermediate hosts and had an immunomodulatory function that regulates the production of inflammatory factors. Thus, in the present research, the recombinant Stefin of C. pisiformis was confirmed to have the potential to fight inflammation in LPS-Mediated RAW264.7 murine macrophages. CCK8 test showed that rCpStefin below 50 μg/mL concentration did not affect cellular viability. Moreover, the NO production level determined by the Griess test was decreased. In addition, the secretion levels of IL-1β, IL-6, and TNF-α as measured by ELISA were decreased. Furthermore, it exerted anti-inflammatory activity by decreasing the production of proinflammatory cytokines and proinflammatory mediators, including IL-1β, IL-6, TNF-α, iNOS, and COX-2 at the gene transcription level, as measured by qRT-PCR. Therefore, Type I cystatin derived from C. pisiformis suppresses the LPS-Mediated inflammatory response of the intermediate host and is a potential candidate for the treatment of inflammatory diseases.
New Insights in Metallurgical Manufacturing in the Caucasian Area: The Case Study of Artefacts from the Samshvilde Citadel Ruins (South Georgia)
Authors: Rovella Natalia , Albanese Maria Pia , Alberghina Maria Francesca , Schiavone Salvatore , Ricca Michela , Berikashvili David , Kvakhadze Levan , Tvaladze Shota , Figoli Alberto , La Russa Mauro Francesco
Published: April 2024

An archaeometric approach was applied to the study of the hoard found in the Samshvilde fortress, one of the richest areas of archaeological artefacts in Caucasian Georgia, since it is representative of the historical events from the Neolithic period until the most recent epochs. In this context, four coins dated back to the 12th–13th centuries AD to the reign of King Giorgi III, Queen Tamar, and King Giorgi IV of Georgia underwent different analytical methods to collect information about the technological production process, the probable forge location, and their conservation state. Optical microscope observations provided details about the decorations, the stylistic aspects, and a preliminary evaluation of the conservation state. Portable X-ray fluorescence and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy analysis revealed a composition consisting almost exclusively of copper with a lower amount of tin, lead, and silver. Moreover, degradation products (copper chlorides and sulphates) and cuprite patina were identified. The metal-supplying areas could be related to the nearby Bolnisi Mining District, and the forge location could coincide with the hoard location in Samshvilde, but further data and analyses are necessary.
Preparation of S-C3N4/AgCdS Z-Scheme Heterojunction Photocatalyst and Its Effectively Improved Photocatalytic Performance
Authors: Lin Yuhong , Chen Zhuoyuan , Feng Chang , Ma Li , Jing Jiangping , Hou Jian , Xu Likun , Sun Mingxian , Chen Dongchu
Published: April 2024

In this study, S-doped graphitic carbon nitride (S-C3N4) was prepared using the high-temperature polymerization method, and then S-C3N4/AgCdS heterojunction photocatalyst was obtained using the chemical deposition method through loading Ag-doped CdS nanoparticles (AgCdS NPs) on the surface of S-C3N4. Experimental results show that the AgCdS NPs were evenly dispersed on the surface of S-C3N4, indicating that a good heterojunction structure was formed. Compared to S-C3N4, CdS, AgCdS and S-C3N4/CdS, the photocatalytic performance of S-C3N4/AgCdS has been significantly improved, and exhibits excellent photocatalytic degradation performance of Rhodamine B and methyl orange. The doping of Ag in collaboration with the construction of a Z-scheme heterojunction system promoted the effective separation and transport of the photogenerated carriers in S-C3N4/AgCdS, significantly accelerated its photocatalytic reaction process, and thus improved its photocatalytic performance.
Temperature-Caused Changes in Raman Pattern and Protein Profiles of Winter Triticale (x Triticosecale, Wittm.) Field-Grown Seedlings
Authors: Stawoska Iwona , Wesełucha-Birczyńska Aleksandra , Golebiowska-Paluch Gabriela
Published: April 2024

Climate change, which causes periods with relatively high temperatures in winter in Poland, can lead to a shortening or interruption of the cold hardening of crops. Previous research indicates that cold acclimation is of key importance in the process of acquiring cereal tolerance to stress factors. The objective of this work was to verify the hypothesis that both natural temperature fluctuations and the plant genotype influence the content of metabolites as well as proteins, including antioxidant enzymes and photosystem proteins. The research material involved four winter triticale genotypes, differing in their tolerance to stress under controlled conditions. The values of chlorophyll a fluorescence parameters and antioxidant activity were measured in their seedlings. Subsequently, the contribution of selected proteins was verified using specific antibodies. In parallel, the profiling of the contents of chlorophylls, carotenoids, phenolic compounds, and proteins was carried out by Raman spectroscopy. The obtained results indicate that a better PSII performance along with a higher photosystem II proteins content and thioredoxin reductase abundance were accompanied by a higher antioxidant activity in the field-grown triticale seedlings. The Raman studies showed that the cold hardening led to a variation in photosynthetic dyes and an increase in the phenolic to carotenoids ratio in all DH lines.
The Simultaneous Detection of Dopamine and Uric Acid In Vivo Based on a 3D Reduced Graphene Oxide–MXene Composite Electrode
Authors: Shang Lingjun , Li Ruijiao , Li Haojie , Yu Shuaiqun , Sun Xuming , Yu Yi , Ren Qiongqiong
Published: April 2024

Dopamine (DA) and uric acid (UA) are essential for many physiological processes in the human body. Abnormal levels of DA and UA can lead to multiple diseases, such as Parkinson’s disease and gout. In this work, a three-dimensional reduced graphene oxide–MXene (3D rGO-Ti3C2) composite electrode was prepared using a simple one-step hydrothermal reduction process, which could separate the oxidation potentials of DA and UA, enabling the simultaneous detection of DA and UA. The 3D rGO-Ti3C2 electrode exhibited excellent electrocatalytic activity towards both DA and UA. In 0.01 M PBS solution, the linear range of DA was 0.5–500 µM with a sensitivity of 0.74 µA·µM−1·cm−2 and a detection limit of 0.056 µM (S/N = 3), while the linear range of UA was 0.5–60 µM and 80–450 µM, with sensitivity of 2.96 and 0.81 µA·µM−1·cm−2, respectively, and a detection limit of 0.086 µM (S/N = 3). In 10% fetal bovine serum (FBS) solution, the linear range of DA was 0.5–500 µM with a sensitivity of 0.41 µA·µM−1·cm−2 and a detection limit of 0.091 µM (S/N = 3). The linear range of UA was 2–500 µM with a sensitivity of 0.11 µA·µM−1·cm−2 and a detection limit of 0.6 µM (S/N = 3). The modified electrode exhibited advantages such as high sensitivity, a strong anti-interference capability, and good repeatability. Furthermore, the modified electrode was successfully used for DA measurement in vivo. This could present a simple reliable route for neurotransmitter detection in neuroscience.
Structure Effects on Swelling Properties of Hydrogels Based on Sodium Alginate and Acrylic Polymers
Authors: Kowalski Grzegorz , Witczak Mariusz , Kuterasiński Łukasz
Published: April 2024

Hydrogels based on sodium alginate (SA) and partially neutralised poly(acrylic acid) were obtained by radical polymerisation. The hydrogels were cross-linked with N,N′-methylenebisacrylamide (MBA), simultaneously grafting the resulting polymer onto SA. The findings of the FTIR spectroscopy showed that all of the hydrogels were effectively synthesized and sodium alginate was chemically bonded with the poly(sodium acrylate) matrix. DSC analysis of the melting heat and glass transition parameters indicated that the hydrogel structure had changed as a result of the cross-linking process. Sodium alginate and MBA were tested at different concentrations to determine how they affected the hydrogel properties. A very high content of the biopolymer, i.e., sodium alginate, was used in our research, up to 33 wt%. This resulted in durable and stable hydrogels with a very high ability to uptake water, comparable to hydrogels based on synthetic polymers only. The ability to swell is inversely proportional to the quantity of MBA present. By increasing the amount of sodium alginate in the hydrogel, the ability of the hydrogel to absorb water is reduced. However, water uptake remains relatively high at 350 g·g−1, even for the hydrogel with the highest SA content.