bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of CT-Based Imaging Signature for Preoperative Prediction of Invasive Behavior in Pancreatic Solid Pseudopapillary Neoplasm
Authors: Huang Wen-peng , Liu Si-yun , Han Yi-jing , Li Li-ming , Liang Pan , Gao Jian-bo
Published: May 2021

Purpose

It is challenging for traditional CT signs to predict invasiveness of pancreatic solid pseudopapillary neoplasm (pSPN). We aim to develop and evaluate CT-based radiomics signature to preoperatively predict invasive behavior in pSPN.

Methods

Eighty-five patients who had pathologically confirmed pSPN and preoperative contrasted-enhanced CT imaging in our hospital were retrospectively analyzed (invasive: 24; non-invasive: 61). 1316 radiomics features were separately extracted from delineated 2D or 3D ROIs in arterial and venous phases. 200% (SMOTE) was used to generate balanced dataset (invasive: 72, non-invasive: 96) for each phase, which was for feature selection and modeling. The model was internally validated in the original dataset. Inter-observer consistency analysis, spearman correlation, univariate analysis, LASSO regression and backward stepwise logical regression were mainly applied to screen the features, and 6 logistic regression models were established based on multi-phase features from 2D or 3D segmentations. The ROC analysis and Delong’s test were mainly used for model assessment and AUC comparison.

Results

It retained 11, 8, 7 and 7 features to construct 3D-arterial, 3D-venous, 2D-arterial and 2D-venous model. Based on 3D ROIs, the arterial model (AUC: 0.914) performed better than venous (AUC: 0.815) and the arterial-venous combined model was slightly improved (AUC: 0.918). Based on 2D ROIs, the arterial model (AUC: 0.814) performed better than venous (AUC:0.768), while the arterial-venous combined model (AUC:0.893) performed better than any single-phase model. In addition, the 3D arterial model performed better than the best combined 2D model. The Delong’s test showed that the significant difference of model AUC existed in arterial models in original dataset (p = 0.019) while not in arterial-venous combined model (p=0.49) as comparing 2D and 3D ROIs.

Conclusion

The arterial radiomics model constructed by 3D-ROI feature is potential to predict the invasiveness of pSPN preoperatively.
Development and Assessment of Screening Nomogram for Biliary Atresia Based on Hepatobiliary Ultrasonographic Features
Authors: Dai Shu Yang , Sun Yu Qi , Wu Ying , Chen Gong , Sun Song , Dong Rui , Zheng Shan
Published: May 2021

Objectives: Biliary atresia (BA) is a rare neonatal liver disease of which the early diagnosis remains a challenge for clinicians. Our center has established a nomogram diagnostic model based on clinical characteristics and liver function characteristics. We aim to develop and validate a nomogram that includes additional ultrasound and finds hepatobiliary abnormality with better BA early screening performance.

Methods: In this single-center, retrospective cohort analysis, 1,001 neonatal obstructive jaundice (NOJ) patients between 2012 and 2015 were enrolled. Multivariable analysis was used to identify clinical characteristics, laboratory liver function characteristics, and ultrasonic features that may early screen BA. A nomogram was developed to predict the probability of BA using multiple logistic regression analysis. This nomogram was subsequently validated using another cohort of 501 NOJ patients between 2015 and 2017. Calibration curve analysis and decision curve analyses were performed to evaluate and interpret the nomogram's clinical benefits.

Results: Gender, direct bilirubin (DB), alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GGT), fasting gallbladder visibility, fasting gallbladder filling, and common bile duct visibility were found to have profound statistical significance between the BA and non-BA groups (P < 0.05). The significant features were used to build the nomogram. The area under the receiver operating characteristic (ROC) curve (AUC) value of the novel nomogram (0.87) was superior to those of the former nomogram (0.83) and GGT alone (0.81) in the prediction of BA. The calibration curve revealed a close resemblance between the predicted and actual BA probabilities. Also, the net benefit from the decision curve analysis (DCA) of the nomogram (0.54) was superior to those of the former nomogram (0.49) and GGT alone (0.45) at 80% of threshold possibility.

Conclusions: The nomogram has demonstrated better performance for BA screening by including additional information of the US finding, holding a promising future as a non-invasive method for BA patients.
Ang II Promotes Cardiac Autophagy and Hypertrophy via Orai1/STIM1
Authors: Zheng Chang-Bo , Gao Wen-Cong , Xie Mingxu , Li Zhichao , Ma Xin , Song Wencong , Luo Dan , Huang Yongxiang , Yang Jichen , Zhang Peng , Huang Yu , Yang Weimin , Yao Xiaoqiang
Published: May 2021

The pathophysiology of cardiac hypertrophy is complex and multifactorial. Both the store-operated Ca2+ entry (SOCE) and excessive autophagy are the major causative factors for pathological cardiac hypertrophy. However, it is unclear whether these two causative factors are interdependent. In this study, we examined the functional role of SOCE and Orai1 in angiotensin II (Ang II)-induced autophagy and hypertrophy using in vitro neonatal rat cardiomyocytes (NRCMs) and in vivo mouse model, respectively. We show that YM-58483 or SKF-96365 mediated pharmacological inhibition of SOCE, or silencing of Orai1 with Orail-siRNA inhibited Ang II-induced cardiomyocyte autophagy both in vitro and in vivo. Also, the knockdown of Orai1 attenuated Ang II-induced pathological cardiac hypertrophy. Together, these data suggest that Ang II promotes excessive cardiomyocyte autophagy through SOCE/Orai1 which can be the prime contributing factors in cardiac hypertrophy.
In silico Study of Trianthema portulacastrum Embedded Iron Oxide Nanoparticles on Glycogen Synthase Kinase-3β: A Possible Contributor to its Enhanced in vivo Wound Healing Potential
Authors: Yadav Ekta , Yadav Pankajkumar , Verma Amita
Published: May 2021

Rich amount of phenolic compounds are available in Trianthema portulacastrum L. (TP) leaves and are traditionally utilized as a wound dressing material. Oxidative stress and inflammation affect the Wnt/β-catenin pathway by modulating the glycogen synthase kinase-3β (GSK) activity subjected to delay in wound healing. The objective of the current study was to explore the wound healing effect of ferric oxide nanoparticles biosynthesized with fractionated TP extract (FeTP). The ability of TP active components (polyphenols) to inhibit the GSK was explored by using molecular docking studies. FeTP were synthesized, characterized, utilized to prepare an ointment and its efficacy was investigated against full-thickness dermal wounds. Different wound healing parameters, level of enzymatic antioxidants, hydroxyproline content and tissue cytokines level were analyzed. Histopathology was performed to confirm the healing by newly formed tissue architecture. Rats treated with FeTP showed significantly swift healing with faster wound contraction rate, high tensile strength and hydroxyproline content along with the utilization of less time for epithelialization. Histopathological study also validated the potential wound healing effect of FeTP with complete re-epithelialization. The results of the present study cumulatively revealed that the green synthesized FeTP ointment approach may serve as a potential tool for dermal wound healing.
Galectins in the Pathogenesis of Common Retinal Disease
Authors: Caridi Bruna , Doncheva Dilyana , Sivaprasad Sobha , Turowski Patric
Published: May 2021

Diseases of the retina are major causes of visual impairment and blindness in developed countries and, due to an ageing population, their prevalence is continually rising. The lack of effective therapies and the limitations of those currently in use highlight the importance of continued research into the pathogenesis of these diseases. Vascular endothelial growth factor (VEGF) plays a major role in driving vascular dysfunction in retinal disease and has therefore become a key therapeutic target. Recent evidence also points to a potentially similarly important role of galectins, a family of β-galactoside-binding proteins. Indeed, they have been implicated in regulating fundamental processes, including vascular hyperpermeability, angiogenesis, neuroinflammation, and oxidative stress, all of which also play a prominent role in retinopathies. Here, we review direct evidence for pathological roles of galectins in retinal disease. In addition, we extrapolate potential roles of galectins in the retina from evidence in cancer, immune and neuro-biology. We conclude that there is value in increasing understanding of galectin function in retinal biology, in particular in the context of the retinal vasculature and microglia. With greater insight, recent clinical developments of galectin-targeting drugs could potentially also be of benefit to the clinical management of many blinding diseases.
MHD Modeling of Solar Coronal Magnetic Evolution Driven by Photospheric Flow
Authors: Jiang Chaowei , Bian Xinkai , Sun Tingting , Feng Xueshang
Published: May 2021

It is well-known that magnetic fields dominate the dynamics in the solar corona, and new generation of numerical modeling of the evolution of coronal magnetic fields, as featured with boundary conditions driven directly by observation data, are being developed. This paper describes a new approach of data-driven magnetohydrodynamic (MHD) simulation of solar active region (AR) magnetic field evolution, which is for the first time that a data-driven full-MHD model utilizes directly the photospheric velocity field from DAVE4VM. We constructed a well-established MHD equilibrium based on a single vector magnetogram by employing an MHD-relaxation approach with sufficiently small kinetic viscosity, and used this MHD equilibrium as the initial conditions for subsequent data-driven evolution. Then we derived the photospheric surface flows from a time series of observed magentograms based on the DAVE4VM method. The surface flows are finally inputted in time sequence to the bottom boundary of the MHD model to self-consistently update the magnetic field at every time step by solving directly the magnetic induction equation at the bottom boundary. We applied this data-driven model to study the magnetic field evolution of AR 12158 with SDO/HMI vector magnetograms. Our model reproduced a quasi-static stress of the field lines through mainly the rotational flow of the AR's leading sunspot, which makes the core field lines to form a coherent S shape consistent with the sigmoid structure as seen in the SDO/AIA images. The total magnetic energy obtained in the simulation matches closely the accumulated magnetic energy as calculated directly from the original vector magnetogram with the DAVE4VM derived flow field. Such a data-driven model will be used to study how the coronal field, as driven by the slow photospheric motions, reaches a unstable state and runs into eruptions.
Conceptual Graphs and Terminological Idiosyncrasy in UNCLOS and CBD
Authors: Mazzega Pierre
Published: May 2021

Do two conventions of international environmental law necessarily endow the same word with the same meaning? A single counterexample is enough to answer in the negative: this is the case of the term “resource” in the United Nations Convention on the Law of the Sea (UNCLOS) and the Convention on Biological Diversity (CBD). Beyond this result, we tackle the questions, raised by the method of analysis implemented, about the semantics of legal texts, a source of interpretative flexibility but also of cognitive amalgamations and confusions of various types. A conceptual graph is associated with each proposition or sentence comprising the term “resource.” Some expressions, especially those of a deontic nature and noun phrases naming a group of interrelated entities or a fact, are encoded in nested graphs. The scope of a term is revealed by the neighbourhood of its uses. Neighbouring expressions, positioned along the paths of conceptual graphs, are ranked owing to their distance from the target expression. Then the neighbours the most contributing to the distributional meaning of the targets are classified in a coarse taxonomy, providing basic ontological traits to “resource” and related expressions in each convention. Although the two conventions rely on the same language, the weak overlap of their respective neighbourhoods of the term “resource” and associated expressions and their contrasted ontological anchorages highlight idiosyncratic meanings and, consequently, divergent orientations and understandings regarding the protection and conservation of resources, especially of living resources. Thus, the complexity of legal texts operates both in the gap between language semantics and cognitive understanding of the concepts used, and in the interpretative flexibility and opportunities for confusion that the texts offer but that the elementary operations of formalisation allow to deconstruct and clarify.
The Role of Environmental Conditions on Master Marathon Running Performance in 1,280,557 Finishers the ‘New York City Marathon’ From 1970 to 2019
Authors: Knechtle Beat , McGrath Carlyn , Goncerz Olivia , Villiger Elias , Nikolaidis Pantelis Theodoros , Marcin Thimo , Sousa Caio Victor
Published: May 2021

Aim: This study investigated the influence of weather conditions on running performance in female and male age group runners in the largest marathon in the world, the “New York City Marathon.”

Methods: The analysis included data from 1,280,557 finishers the “New York City Marathon” from the years 1970 to 2019. Linear mixed models for men and women finishers with race time (min) as dependent variable and 5-year age groups, temperature, wind and relative humidity tertiles (low, medium, high) as independent factors and finisher as random intercept was performed. Additional models with an interaction between age groups and one weather variable each were performed.

Results: Temperature was positively associated with race time while wind speed and humidity were negatively associated (p < 0.001). Men were significantly greater affected wind speed and humidity than women (p < 0.001 for interaction) but not by temperature (p = 0.17 for interaction). With an average of 8 min longer race time, high temperature had the greatest effect on race time. The effect of high humidity on race time was significantly increased in 40–59 years old men and 25–65 years old women. High temperatures had an increased effect on race time in 30–64 years old men and 40–64 years old women. The inverse association between race time and high wind speed was pronounced in finishers with younger age.

Conclusion: Performance was lower on days with high temperature, low humidity and low wind speed. Men seemed to benefit more from higher humidity and wind speed than women. Aged (70 +) finishers were not greater affected by high temperatures.
Deciphering Genotype-By-Environment Interaction for Target Environmental Delineation and Identification of Stable Resistant Sources Against Foliar Blast Disease of Pearl Millet
Authors: Sankar S. Mukesh , Singh S. P. , Prakash G. , Satyavathi C. Tara , Soumya S. L. , Yadav Yashpal , Sharma L. D. , Rao A. R. , Singh Nirupma , Srivastava Rakesh K.
Published: May 2021

Once thought to be a minor disease, foliar blast disease of pearl millet, caused by Magnaporthe grisea, has recently emerged as an important biotic constraint for pearl millet production in India. The presence of a wider host range as well as high pathogenic heterogeneity complicates host–pathogen dynamics. Furthermore, environmental factors play a significant role in exacerbating the disease severity. An attempt was made to unravel the genotype-by-environment interactions for identification and validation of stable resistant genotypes against foliar blast disease through multi-environment testing. A diversity panel consisting of 250 accessions collected from over 20 different countries was screened under natural epiphytotic conditions in five environments. A total of 43 resistant genotypes were found to have high and stable resistance. Interestingly, most of the resistant lines were late maturing. Combined ANOVA of these 250 genotypes exhibited significant genotype-by-environment interaction and indicated the involvement of crossover interaction with a consistent genotypic response. This justifies the necessity of multi-year and multi-location testing. The first two principal components (PCs) accounted for 44.85 and 29.22% of the total variance in the environment-centered blast scoring results. Heritability-adjusted genotype plus genotype × environment interaction (HA-GGE) biplot aptly identified “IP 11353” and “IP 22423, IP 7910 and IP 7941” as “ideal” and “desirable” genotypes, respectively, having stable resistance and genetic buffering capacity against this disease. Bootstrapping at a 95% confidence interval validated the recommendations of genotypes. Therefore, these genotypes can be used in future resistance breeding programs in pearl millet. Mega-environment delineation and desirability index suggested Jaipur as the ideal environment for precise testing of material against the disease and will increase proper resource optimization in future breeding programs. Information obtained in current study will be further used for genome-wide association mapping of foliar blast disease in pearl millet.
Extreme Growth Increments Reveal Local and Regional Climatic Signals in Two Pinus pinaster Populations
Authors: Vieira Joana , Nabais Cristina , Campelo Filipe
Published: May 2021

Tree rings are valuable proxies of past climate that allow inferring past growth responses to climate variability and extreme events, which is only possible considering that the relationship between tree growth and environmental conditions is linear and stable over time. However, in the last decades, divergent growth patterns have been observed in trees from the same forest stand, while unprecedented growth convergence was observed between trees from distant locations. Here, we use a new approach that considers convergent and divergent event years in two populations of Pinus pinaster Aiton in an altitudinal and oceanic-continental gradient to investigate what is triggering divergence and convergence in tree growth. The two study sites are Tocha (TCH), a plantation on sand dunes at low altitude near the ocean, and Serra da Estrela (SdE), a mountain plantation located at 1,100 m altitude, 100 km away from the ocean. The analysis of the climatic conditions in convergent growth years revealed that positive convergent growth was related to above average precipitation in previous winter and that negative convergent growth was related to below average precipitation during the growing season. Divergent growth revealed a temperature signal with warmer temperatures in spring promoting growth in SdE and growth reduction in TCH. Convergent growth was associated with a regional climatic signal, reinforcing the importance of precipitation in the Mediterranean region, and divergent growth to site conditions, revealing local adaptation. The information gathered in this study gives valuable insights on the response of P. pinaster to extreme climatic events, allowing for more adjusted management strategies of Mediterranean pine forests.
Combining QTL Mapping and Transcriptomics to Decipher the Genetic Architecture of Phenolic Compounds Metabolism in the Conifer White Spruce
Authors: Laoué Justine , Depardieu Claire , Gérardi Sébastien , Lamothe Manuel , Bomal Claude , Azaiez Aïda , Gros-Louis Marie-Claude , Laroche Jérôme , Boyle Brian , Hammerbacher Almuth , Isabel Nathalie , Bousquet Jean
Published: May 2021

Conifer forests worldwide are becoming increasingly vulnerable to the effects of climate change. Although the production of phenolic compounds (PCs) has been shown to be modulated by biotic and abiotic stresses, the genetic basis underlying the variation in their constitutive production level remains poorly documented in conifers. We used QTL mapping and RNA-Seq to explore the complex polygenic network underlying the constitutive production of PCs in a white spruce (Picea glauca) full-sib family for 2 years. QTL detection was performed for nine PCs and differentially expressed genes (DEGs) were identified between individuals with high and low PC contents for five PCs exhibiting stable QTLs across time. A total of 17 QTLs were detected for eight metabolites, including one major QTL explaining up to 91.3% of the neolignan-2 variance. The RNA-Seq analysis highlighted 50 DEGs associated with phenylpropanoid biosynthesis, several key transcription factors, and a subset of 137 genes showing opposite expression patterns in individuals with high levels of the flavonoids gallocatechin and taxifolin glucoside. A total of 19 DEGs co-localized with QTLs. Our findings represent a significant step toward resolving the genomic architecture of PC production in spruce and facilitate the functional characterization of genes and transcriptional networks responsible for differences in constitutive production of PCs in conifers.
The Influence of Language on Spatial Reasoning: Reading Habits Modulate the Formulation of Conclusions and the Integration of Premises
Authors: Castelain Thomas , Van der Henst Jean-Baptiste
Published: May 2021

In the present study, we explore how reading habits (e.g., reading from left to right in French or reading from right to left in Arabic) influence thescanningand theconstructionof mental models in spatial reasoning. For instance, when participants are given a problem like A is to the left of B; B is to the left of C, what is the relation between A and C? They are assumed to construct the model: A B C. If reading habits influence the scanning process, then readers of French should inspect models from left to right, whereas readers of Arabic should inspect them from right to left. The prediction following this analysis is that readers of French should be more inclined to produce “left” conclusions (i.e., A is to the left of C), whereas readers of Arabic should be more inclined to produce “right” conclusions (i.e., C is to the right of A). Furthermore, one may expect that readers of French show a greater ease in constructing models following a left-to-right direction than models following a right-to-left direction, whereas an opposite pattern might be expected for readers of Arabic. We tested these predictions in two experiments involving French and Yemeni participants. Experiment 1 investigated the formulation of conclusions from spatial premises, and Experiment 2, which was based on non-linguistic stimuli, examined the time required to construct mental models from left to right and from right to left. Our results show clear differences between the two groups. As expected, the French sample showed a strong left-to-right bias, but the Yemeni sample did not show the reverse bias. Results are discussed in terms of cultural influences and universal mechanisms.
“We Thought We Were Alone”: The Subjective Experience of the Siblings of Anorexic Adolescent Patients
Authors: Persico Angelo , Grandclerc Salome , Giraud Catherine , Moro Marie Rose , Blanchet Corinne
Published: May 2021

Objective: The siblings of patients suffering from Anorexia Nervosa (AN) are potentially affected by a disturbed emotional experience that often remains undetected. In order to bring them a psychological support, the Maison de Solenn proposed a support group program for these siblings. The current research explores their mental representations of AN and their emotional experience in the support group named “sibling group.”

Method: This exploratory study is based on a phenomenological and inductive qualitative method. Four girls and three boys aged between 6 and 19 participating in the “sibling group” were included in a one-time focus group session using a semi-structured interview guide. The thematic data analysis was performed by applying the methods of interpretative phenomenological analysis.

Results: Themes that emerged from the interview fall into four categories: AN explained by siblings; the individual emotional experience of siblings; the family experience of siblings and the experience inside the “sibling group.”

Discussion: According to our participants, the “sibling group” thus functions as a good compromise between keeping an active role in the anorexic patient's care and taking a step back to avoid being eaten up by the illness. Sibling-group participants retrieved a sense of belonging, which is normally one of the functions of being a sibling. It is important to note that the “sibling group” is part of the comprehensive (or global) family-based approach included in an institutional multidisciplinary integrative care framework.
Farm Production Diversity and Household Dietary Diversity: Panel Data Evidence From Rural Households in Tanzania
Authors: Habtemariam Lemlem Teklegiorgis , Gornott Christoph , Hoffmann Harry , Sieber Stefan
Published: May 2021

Evidence on whether diversifying farm production leads to improved household dietary diversity and nutrition remains inconclusive. Existing studies analyzing the link between production diversity and dietary diversity are mainly based on cross-sectional methods, which could be biased by omitted confounding factors. Using two waves of a panel household survey of 900 rural households in Tanzania, this paper examines the link between production diversity and dietary diversity, while minimizing potential confounding effects. We estimate four regression models with two different production diversity measures and two panel estimation methods—fixed effect (FE) and random effect (RE). In three out of the four models, production diversity is significantly and positively associated with the dietary diversity measure of the food consumption score. The production diversity indicator is represented by the total crop and livestock species count, as well as by counting only crop species. The total crop and livestock species count shows a significant positive association with dietary diversity across estimation methods while the positive association with crop species count is not significant in the FE method. Our results suggest that the selection of appropriate production diversity indicators tailored to the specific circumstances of the local agricultural system is likely one key factor in identifying a robust relationship between production diversity and dietary diversity.
Urinary Cell-Free miR-99a-5p as a Potential Biomarker for Estrus Detection in Buffalo
Authors: Hebbar Aparna , Chandel Rajeev , Rani Payal , Onteru Suneel Kumar , Singh Dheer
Published: May 2021

Accurate estrus detection method is the need of the hour to improve reproductive efficiency of buffaloes in dairy industry, as the currently available estrus detection methods/tools lack high sensitivity and specificity. Recently, circulating miRNAs have been shown as non-invasive biomarkers by various studies. Hence, in order to evaluate their potential as estrus biomarkers, the objective of this study was to identify and compare the levels of 10 hormone-responsive miRNAs in the urine collected at proestrus (PE), estrus (E), and diestrus (DE) phases of buffaloes (n = 3) pertaining to a discovery sample. Among 10 urinary miRNAs, the levels of bta-mir-99a-5p (E/PE 0.5-fold, P < 0.05; DE/PE 1.9-fold), bta-miR-125b (E/PE 0.5-fold; DE/PE 0.7-fold), bta-mir-145 (E/PE 1.5-fold; DE/PE 0.7-fold), bta-mir-210 (E/PE 1.2-fold, DE/PE 0.7-fold), mir-21 (E/PE 1.5-fold, DE/PE 2-fold), and bta-mir-191 (E/PE 1.3-fold; DE/PE 0.8-fold) were found to be altered during different phases of buffalo estrous cycle. In contrast, bta-mir-126-3p, bta-let-7f, bta-mir-16b, and bta-mir-378 were undetected in buffalo urine. Furthermore, a validation study in an independent group of 25 buffalo heifers showed the increased levels of urinary bta-mir-99a-5p during the DE (3.92-fold; P < 0.0001) phase as compared to the E phase. Receiver operating characteristic curve analyses also revealed the ability of urinary miR-99a-5p in distinguishing the E from the DE phase (area under the curve of 0.6464; P < 0.08). In silico analysis further showed an enrichment of miR-99a-5p putative targets in various ovarian signaling pathways, including androgen/estrogen/progesterone biosynthesis and apoptosis signaling, implicating the role of miR-99a-5p in ovarian physiology. In conclusion, significantly lower levels of bta-mir-99a-5p at the E phase than the DE phase in buffalo urine indicate its biomarker potential, which needs to be further explored in a large cohort in the future studies.
A Comparison of Parapoxviruses in North American Pinnipeds
Authors: Costa Helena , Klein Jörn , Breines Eva M. , Nollens Hendrik H. , Matassa Keith , Garron Mendy , Duignan Padraig J. , Schmitt Todd , Goldstein Tracey , Tryland Morten
Published: May 2021

Parapoxviruses cause nodular lesions on the skin and mucosal membranes of pinnipeds and infections by these viruses have been documented worldwide. Seal parapoxvirus is currently classified as a tentative species of the Parapoxvirus genus. Tissue or swab samples were analyzed from 11 pinnipeds of different host species undergoing rehabilitation on the east and west coasts of the United States of America (USA) that were positive for parapoxvirus. The aim of the study was to compare parapoxvirus sequences of fragments of the B2L, DNA polymerase, GIF and viral interleukin-10 ortholog (vIL-10) genes and to examine the evolutionary relationship between viruses detected in different pinniped species and at different locations with other members of the Parapoxvirus genus, such as Orf virus (ORFV), Bovine papular stomatitis virus (BPSV) and Pseudocowpox virus (PCPV). The sequence analysis showed that the parapoxvirus sequences from the pinnipeds differed significantly from those found in terrestrial hosts and that they formed a separate cluster within the genus. Our results suggest that transmission of the same parapoxvirus strain is possible between different species, including between members of different families (phocids and otariids). Animals belonging to the same species but living in distant geographic locations presented genetically distant parapoxviruses. The findings of this study demonstrate that sealpox lesions in pinnipeds of different species are caused by viruses that belong to the Parapoxvirus genus but have significant genetic differences compared to the established virus species in terrestrial hosts, thus strongly supporting the classification of pinniped parapoxvirus as a new species of the genus.
Characterising Foot-and-Mouth Disease Virus in Clinical Samples Using Nanopore Sequencing
Authors: Brown Emma , Freimanis Graham , Shaw Andrew E. , Horton Daniel L. , Gubbins Simon , King David
Published: May 2021

The sequencing of viral genomes provides important data for the prevention and control of foot-and-mouth disease (FMD) outbreaks. Sequence data can be used for strain identification, outbreak tracing, and aiding the selection of the most appropriate vaccine for the circulating strains. At present, sequencing of FMD virus (FMDV) relies upon the time-consuming transport of samples to well-resourced laboratories. The Oxford Nanopore Technologies' MinION portable sequencer has the potential to allow sequencing in remote, decentralised laboratories closer to the outbreak location. In this study, we investigated the utility of the MinION to generate sequence data of sufficient quantity and quality for the characterisation of FMDV serotypes O, A, Asia 1. Prior to sequencing, a universal two-step RT-PCR was used to amplify parts of the 5′UTR, as well as the leader, capsid and parts of the 2A encoding regions of FMDV RNA extracted from three sample matrices: cell culture supernatant, tongue epithelial suspension and oral swabs. The resulting consensus sequences were compared with reference sequences generated on the Illumina MiSeq platform. Consensus sequences with an accuracy of 100% were achieved within 10 and 30 min from the start of the sequencing run when using RNA extracted from cell culture supernatants and tongue epithelial suspensions, respectively. In contrast, sequencing from swabs required up to 2.5 h. Together these results demonstrated that the MinION sequencer can be used to accurately and rapidly characterise serotypes A, O, and Asia 1 of FMDV using amplicons amplified from a variety of different sample matrices.
Health in Conflict Zones: Analyzing Inequalities in Mental Health in Colombian Conflict-Affected Territories
Authors: León-Giraldo Sebastián , Casas Germán , Cuervo-Sanchez Juan Sebastian , González-Uribe Catalina , Bernal Oscar , Moreno-Serra Rodrigo , Suhrcke Marc
Published: May 2021

Objectives: Colombia’s civil conflict and persistent socio-economic disparities have contributed to mental health inequalities in conflict-affected territories. We explore the magnitude of mental health inequalities, contributing socio-economic factors, and sociodemographic characteristics that explain these differences.

Methods: The study draws on data collected in 2018, using the household survey Conflicto, Paz y Salud (CONPAS) applied to 1,309 households in Meta, Colombia. Logistic regression and decomposition analysis were used to analyze the risk of mental health disorders, measured with the Self-Reporting Questionnaire -20 (SRQ-20).

Results: Individuals with lower socio-economic status are at a higher risk for mental health disorders. Forced displacement accounts for 31% of the measured mental health inequalities. Disparities in employment, education level, disability and conflict incidence between municipalities are other contributing factors. Women and people with disabilities are respectively 2.3 and 1.2 times more prone to present a mental health disorder.

Conclusion: It is necessary to tackle the identified risk factors and sociodemographic circumstances that contribute to mental health inequalities in conflict-affected territories, as these hinder adequate/equitable access to mental health services.
Three-Surface Model with Redundant Longitudinal Control: Modeling, Trim Optimization and Control in a Preliminary Design Perspective
Authors: Cacciola Stefano , Riboldi Carlo , Arnoldi Matteo
Published: May 2021

Notwithstanding the interest in the three-surface concept shown by aircraft designers, this configuration was not thoroughly investigated in conjunction with the adoption of two-elevator surfaces, on both canard and tail. In fact, the inclusion of an additional elevator produces a redundant longitudinal control which can be specifically exploited to target trim optimization. The same redundancy can be also employed to improve the flying qualities of the three-surface aircraft. In this paper, after introducing a simple flight mechanics model, ideal for preliminary design and analyses, the advantages of this configuration are explored. Firstly, the problem of finding the elevator deflections of canard and tail for minimum drag in trim is formulated and solved. Secondarily, the updating of a two-surface back-tailed airplane into an equivalent three-surface one is demonstrated, showing the potential improvement in cruise performance. Finally, the controls are employed through a smart control law for achieving better flying qualities.
Impact of an Antimicrobial Stewardship Program on the Incidence of Carbapenem Resistant Gram-Negative Bacilli: An Interrupted Time-Series Analysis
Authors: López-Viñau Teresa , Peñalva Germán , García-Martínez Lucrecia , Castón Juan , Muñoz-Rosa Montserrat , Cano Ángela , Recio Manuel , Cisneros José , Pérez-Nadales Elena , Rumbao Aguirre José , García-Martínez Elena , Salcedo Inmaculada , del Prado José , de la Fuente Carmen , Martínez-Martínez Luis , Gracia-Ahufinger Irene , Torre-Cisneros Julián
Published: May 2021

Carbapenem-resistant Gram-negative bacilli (CR-GNB) are a critical public health threat, and carbapenem use contributes to their spread. Antimicrobial stewardship programs (ASPs) have proven successful in reducing antimicrobial use. However, evidence on the impact of carbapenem resistance remains unclear. We evaluated the impact of a multifaceted ASP on carbapenem use and incidence of CR-GNB in a high-endemic hospital. An interrupted time-series analysis was conducted one year before and two years after starting the ASP to assess carbapenem consumption, CR-GNB incidence, death rates of sentinel events, and other variables potentially related to CR-GNB incidence. An intense reduction in carbapenem consumption occurred after starting the intervention and was sustained two years later (relative effect −83.51%; 95% CI −87.23 to −79.79). The incidence density of CR-GNB decreased by −0.915 cases per 1000 occupied bed days (95% CI −1.743 to −0.087). This effect was especially marked in CR-Klebsiella pneumoniae and CR-Escherichia coli, reversing the pre-intervention upward trend and leading to a relative reduction of −91.15% (95% CI −105.53 to −76.76) and −89.93% (95% CI −107.03 to −72.83), respectively, two years after starting the program. Death rates did not change. This ASP contributed to decreasing CR-GNB incidence through a sustained reduction in antibiotic use without increasing mortality rates.
A Pilot Randomised Clinical Trial Comparing a Short-Term Perioperative Prophylaxis Regimen to a Long-Term Standard Protocol in Equine Colic Surgery
Authors: Stöckle Sabita Diana , Kannapin Dania A. , Kauter Anne M. L. , Lübke-Becker Antina , Walther Birgit , Merle Roswitha , Gehlen Heidrun
Published: May 2021

Background: For surgical interventions classified as clean or clean-contaminated, including laparotomy, guidelines in human and veterinary medicine recommend a short-term perioperative antibiotic prophylaxis (PAP). In equine colic surgery, however, PAP commonly exceeds 24 h. Objectives: The aim of this study was to compare a single-shot to a 5-day lasting PAP considering surgical site infections (SSI) and other adverse effects probably associated with the particular antimicrobial regimen. Study design: The study was designed as a randomised non-inferiority pilot study including horses subjected to colic surgery while receiving one of two distinct PAP regimens. Methods: All horses (n = 67) included in the study received the standard physical examination before and after surgery. Colic surgery was performed according to the current standard of the clinic. Horses were randomly assigned to two groups, receiving either the “single-shot” or the “5-day lasting” antibiotic prophylaxis. The “single-shot” group (n = 30) received penicillin and gentamicin only once before and, if needed, during surgery, whereas the “5-day lasting” group (n = 37) received antibiotics for five days. In addition to the standard laboratory examinations, serum amyloid A and fibrinogen were determined preoperatively and during five days after surgery. SSI, postoperative colitis and haemolytic anaemia were classified as postoperative complications potentially related to antibiotic use. Results: The outcome of this preliminary non-inferiority clinical trial showed that the occurrence of postoperative adverse events (i.e., SSI, postoperative colitis and haemolytic anaemia) lacked significant differences between the study groups. Main limitations: The main limitations of this study are the limited group sizes and our inability to blind the study. Conclusions: Single-shot PAP seems to be an alternative approach considering the 5-day lasting protocol commonly used in equine abdominal surgery. However, a proper hygiene management together with a close clinical and laboratory monitoring of the equine patient is indispensable.
Insight on the Structure-to-Activity of Carbosilane Metallodendrimers in the Fight against Staphylococcus aureus Biofilms
Authors: Llamazares Celia , Sanz del Olmo Natalia , Soliveri Juan , de la Mata F. Javier , Copa-Patiño José Luis , García-Gallego Sandra
Published: May 2021

Biofilm formation is a critical health concern, involved in most human bacterial infections. Combatting this mechanism, which increases resistance to traditional antibiotics and host immune defences, requires novel therapeutic approaches. The remarkable biocide activity and the monodispersity of carbosilane metallodendrimers make them excellent platforms to evaluate the impact of different structural parameters on the biological activity. In this work, we explore the influence of iminopyridine ring substituents on the antibacterial activity against planktonic and biofilm Staphylococcus aureus. New families of first-generation Ru(II) and Cu(II) metallodendrimers were synthesised and analysed, in comparison to the non-substituted counterparts. The results showed that the presence of methyl or methoxy groups in meta position to the imine bond decreased the overall positive charge on the metal ion and, subsequently, the activity against planktonic bacteria. However, it seemed a relevant parameter to consider for the prevention of biofilm formation, if they contribute to increasing the overall lipophilicity. An optimum balance of the charge and lipophilicity of the metallodrug, accomplished through structural design, will provide effective biocide agents against bacteria biofilms.
Diabetic Retinopathy and NADPH Oxidase-2: A Sweet Slippery Road
Authors: Kowluru Renu A.
Published: May 2021

Diabetic retinopathy remains the leading cause of vision loss in working-age adults. The multi-factorial nature of the disease, along with the complex structure of the retina, have hindered in elucidating the exact molecular mechanism(s) of this blinding disease. Oxidative stress appears to play a significant role in its development and experimental models have shown that an increase in cytosolic Reacttive Oxygen Speies (ROS) due to the activation of NADPH oxidase 2 (Nox2), is an early event, which damages the mitochondria, accelerating loss of capillary cells. One of the integral proteins in the assembly of Nox2 holoenzyme, Rac1, is also activated in diabetes, and due to epigenetic modifications its gene transcripts are upregulated. Moreover, addition of hyperlipidemia in a hyperglycemic milieu (type 2 diabetes) further exacerbates Rac1-Nox2-ROS activation, and with time, this accelerates and worsens the mitochondrial damage, ultimately leading to the accelerated capillary cell loss and the development of diabetic retinopathy. Nox2, a multicomponent enzyme, is a good candidate to target for therapeutic interventions, and the inhibitors of Nox2 and Rac1 (and its regulators) are in experimental or clinical trials for other diseases; their possible use to prevent/halt retinopathy will be a welcoming sign for diabetic patients.
Oxidative Stability, Microbial Safety, and Sensory Properties of Flaxseed (Linum usitatissimum L.) Oil Infused with Spices and Herbs
Authors: Odeh Dyana , Kraljić Klara , Benussi Skukan Andrea , Škevin Dubravka
Published: May 2021

In our study, we assessed whether the addition of basil, fennel, oregano, rosemary, and chili can improve oxidative stability and sensory properties of flaxseed oil (FO) during 180 days of storage or induce oil contamination by microorganisms. Results showed that addition of spices and herbs in FO affected the hydrolytic changes, but far less than 2% of free fatty acids after storage, which was in line with regulations. Further, the addition of spices and herbs in FO decreased peroxide value (even up to 68.7% in FO with oregano) vs. FO whose value increased during storage, indicating increased oxidative stability and prolongation of shelf life of infused oils. The antioxidant activity of the infused oils ranged from 56.40% to 97.66%. In addition, the phenol content was higher in all infused oils (6.81–22.92 mg/kg) vs. FO (5.44 mg/kg), indicating that herbs and spices could scavenge free radicals and inhibit lipid peroxidation, while sensory analysts showed that FO infused with chili had the lowest bitterness intensity. According to the presence of certain microorganisms, results highlighted the need to develop new methods for inactivating microorganisms that would not only provide a microbial safety, but also preserve the beneficial properties of the oils/products.
Mitophagy and Oxidative Stress: The Role of Aging
Authors: De Gaetano Anna , Gibellini Lara , Zanini Giada , Nasi Milena , Cossarizza Andrea , Pinti Marcello
Published: May 2021

Mitochondrial dysfunction is a hallmark of aging. Dysfunctional mitochondria are recognized and degraded by a selective type of macroautophagy, named mitophagy. One of the main factors contributing to aging is oxidative stress, and one of the early responses to excessive reactive oxygen species (ROS) production is the induction of mitophagy to remove damaged mitochondria. However, mitochondrial damage caused at least in part by chronic oxidative stress can accumulate, and autophagic and mitophagic pathways can become overwhelmed. The imbalance of the delicate equilibrium among mitophagy, ROS production and mitochondrial damage can start, drive, or accelerate the aging process, either in physiological aging, or in pathological age-related conditions, such as Alzheimer’s and Parkinson’s diseases. It remains to be determined which is the prime mover of this imbalance, i.e., whether it is the mitochondrial damage caused by ROS that initiates the dysregulation of mitophagy, thus activating a vicious circle that leads to the reduced ability to remove damaged mitochondria, or an alteration in the regulation of mitophagy leading to the excessive production of ROS by damaged mitochondria.
Effect of Dietary Grape Pomace on Fattening Rabbit Performance, Fatty Acid Composition, and Shelf Life of Meat
Authors: Bouzaida Mohamed D. , Resconi Virginia C. , Gimeno David , Romero Jakeline V. , Calanche Juan B. , Barahona Marta , Olleta José L. , María Gustavo A.
Published: May 2021

The use of agroindustry by-products in animal diets allows the use of residues that are not fit for human consumption. In this study, it was investigated whether fattening commercial rabbits during 30 days with a non-medicated feed, with 20% addition of grape pomace (GPD), affected production traits and the fatty acid composition, antioxidants properties, and the shelf life of the meat compared to a conventional strategy (CON). Furthermore, it was tested, by chromatographic analysis, whether this alternative diet allowed the transfer of phenolic compounds to the meat. Thirty-six weaned rabbits were allotted to the two treatments. In each treatment, 18 rabbits were fattened in three indoor cages, each housing three males and three female rabbits. No significant differences were found in live weights (p > 0.05), but the feed conversion rate and carcass weight and yield were found to be impaired in the GPD group (p ≤ 0.05). The GPD group had a higher intramuscular fat percentage (2.01 vs. 1.54), improved polyunsaturated/saturated fatty acids ratio (0.75 vs. 0.66), and better atherogenicity (0.71 vs. 0.83) and thrombogenicity (1.14 vs. 1.24) indexes, while the n-6/n-3 ratio was higher (25.4 vs. 20.3). Total volatile basic nitrogen in meat was lower in the GPD group (p = 0.01), suggesting a delayed spoilage. However, no improvements in total phenolic content, antioxidant capacity, reducing power, and lipid oxidation (p > 0.05) were found in the meat. Even though the GPD pellets offered to the animals had several grape-derived phenolic compounds, and higher antioxidant properties compared to the CON diet, none of the phenolic compounds detected in feeds were detected in the meat samples.