bluuebunny/crossref_metadata_2025 huggingface dataset:
AIEgen-Enabled Multicolor Visualization for the Formation of Supramolecular Polymer Networks
Authors: Xu Shaoyu , Zhang Hanwei , Li Qingyun , Liu Hui , Ji Xiaofan
Published: November 2022

Extensive reports on the use of supramolecular polymer networks (SPNs) in self-healing materials, controlled release system and degradable products have led more researchers to tap their potential owing to the unique properties. Yet, the attendant efforts in the visualization through conventional luminescence methods during the formation of SPNs have been met with limited success. Herein, we designed a special type of SPNs prepared by PPMU polymer chains containing pyrene benzohydrazonate (PBHZ) molecules as AIEgens for the multicolor visualization with naked eyes. The complete detection of the formation process of the networks relied on the PBHZ molecules with aggregation-induced ratiometric emission (AIRE) effect, which enabled the fluorescence of the polymer networks transits from blue to cyan, and then to green with the increasing crosslinking degree derived from the hydrogen bonds between 2-ureido-4-pyrimidone (UPy) units of the polymer chains. Additionally, we certificated the stimuli-responsiveness of the obtained SPNs, and the fluorescence change, as well as observing the morphology transition. The AIEgen-enabled multicolor visualization of the formation of SPNs may provide better understanding of the details of the crosslinking interactions in the microstructural evolution, giving more inspiration for the multifunctional products based on SPNs.
Design and Performance of an Adsorption Bed with Activated Carbons for Biogas Purification
Authors: Molino Giulia , Gandiglio Marta , Fiorilli Sonia , Lanzini Andrea , Drago Davide , Papurello Davide
Published: November 2022

Organic waste can be efficiently converted into energy using highly efficient energy systems, such as SOFCs coupled to the anaerobic digestion process. SOFC systems fed by biogenous fuels, such as biogas or syngas, suffer long-term stability due to trace compound impacts. It follows that, a mandatory gas cleaning section is needed to remove these pollutants at lower concentrations. This work investigates the adsorption mechanism for micro-contaminant removal through experimental results achieved using solid sorbents. Samples of different sorbent materials were analyzed in the laboratory to determine their performances in terms of sulfur (mainly hydrogen sulfide) and siloxanes (mainly D4-Octamethylcyclotetrasiloxane) adsorption capacities. The analysis shows that the chemical composition of the samples influences the adsorption of H2S (i.e., presence of calcium, iron, copper), while the effect of their textural properties mainly influences the adsorption of siloxane compounds, such as D4. A quantitative analysis was performed considering the influence of gas velocity on adsorption capacity. By increasing the biogas velocity (+45% and +89%), there was an indirect correlation with the H2S adsorption capacity (−27% and −44%). This identified an aspect related to the residence time required to be able to remove and retain the trace compound. The results obtained and summarized were used to develop a strategy for the removal of trace compounds in large-scale plants, e.g., for water purification.
Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways
Authors: Yang Wenqian , Shao Fei , Wang Jiexin , Shen Tong , Zhao Yu , Fu Xueyan , Zhang Liming , Li Hangying
Published: November 2022

Background: Immunological liver injury (ILI) is a common liver disease and lacks potent drugs for treatment. Artemisia argyi Lévl. et Vant. (A. argyi), a medicinal and edible homologous plant usually used in diet therapy to cure various liver diseases, provides a great option for the prevention of ILI. Purpose: To investigate the effect that ethyl acetate extract of A. argyi (AaEA) on Concanavalin A (ConA)-induced ILI and the mechanism of regulating Bax/Bcl-2 and TLR4/MyD88/NF-κB signaling pathways. Methods: The chemical components of AaEA were studied by LC-MS. In animal experiments, the positive control group was administrated diammonium glycyrrhizinate (DIG, 100 mg/kg), while different doses of AaEA groups (AaEA-H, AaEA-M, AaEA-L) were pretreated with AaEA 2.00, 1.00, and 0.50 g/kg, respectively, by intragastric for seven days, once every day. Then, ConA (12.00 mg/kg) was used through tail intravenous injection to establish the ILI model. The blood samples and livers were collected to test the degree of liver dysfunction, inflammation, oxidative stress, histopathological changes, and cell apoptosis. Real-time PCR and Western blotting analysis were used to explain the mechanism of regulating Bax/Bcl-2 and TLR4/MyD88/NF-κB signaling pathways. Results: The way in which AaEA prevents liver damage in immunological liver injury (ILI) mice caused by ConA was investigated for the first time. Pretreatment with AaEA reduced the expression of ALT, AST, and inflammatory factors (TNF-α and IFN-γ). Meanwhile, AaEA also reduced MDA levels but upregulated the contents of IL-4, SOD, and GSH-px, alleviating oxidative stress induced by ILI. Western blotting and real-time PCR analysis demonstrated that AaEA could regulate the expression level and relative mRNA expression of key proteins on Bax/Bcl-2 and TLR4/MyD88/NF-κB signaling pathways. Finally, 504 components from AaEA were identified by LC-MS analysis, mainly including flavones, phenolic acids, and terpenoids with anti-inflammatory and liver protective activities, which highlights the potential of AaEA for diet treatment of ILI. Conclusion: AaEA can work against ConA-induced ILI in mice by regulating Bax/Bcl-2 and TLR4/MyD88/NF-κB signaling pathways, which has the potential to be a great strategy for the prevention of ILI.
Aquaphotomic Study of Effects of Different Mixing Waters on the Properties of Cement Mortar
Authors: Muncan Jelena , Tamura Satoshi , Nakamura Yuri , Takigawa Mizuki , Tsunokake Hisao , Tsenkova Roumiana
Published: November 2022

The mixing water used for cement concrete has a significant effect on the physical properties of the material after hardening; however, other than the upper limit for the mixed impurities, not enough consideration has been given to the functions and characteristics of water at the molecular level. In this study, we investigated the effect of four different types of water (two spring-, mineral waters, tap water and distilled water) on the drying shrinkage of the hardened cement by comparing the material properties of the concrete specimens and analyzing the molecular structure of the water and cement mortar using aquaphotomics. The near infrared (NIR) spectra of waters used for mixing were acquired in the transmittance mode using a high-precision, high-accuracy benchtop spectrometer in the range of 400–2500 nm, with the 0.5 nm step. The NIR spectra of cement paste and mortar were measured in 6.2 nm increments in the wavelength range of 950 nm to 1650 nm using a portable spectrometer. The measurements of cement paste and mortar were performed on Day 0 (immediately after mixing, cement paste), 1 day, 3 days, 7 days, and 28 days after mixing (cement mortar). The spectral data were analyzed according to the aquaphotomics’ multivariate analysis protocol, which involved exploration of raw and preprocessed spectra, exploratory analysis, discriminating analysis and aquagrams. The results of the aquaphotomics’ analysis were interpreted together with the results of thermal and drying shrinkage measurements. Together, the findings clearly demonstrated that the thermal and drying shrinkage properties of the hardened cement material differed depending on the water used. Better mechanical properties were found to be a result of using mineral waters for cement mixing despite minute differences in the chemical content. In addition, the aquaphotomic characterization of the molecular structure of waters and cement mortar during the initial hydration reaction demonstrated the possibility to predict the characteristics of hardened cement at a very early stage. This provided the rationale to propose a novel evaluation method based on aquaphotomics for non-invasive evaluation and monitoring of cement mortar.
Influence of Caffeic and Caftaric Acid, Fructose, and Storage Temperature on Furan Derivatives in Base Wine
Authors: Medeiros Jacob , Xu Shufen , Pickering Gary J. , Kemp Belinda S.
Published: November 2022

The aim of this study was to determine the influence of caffeic and caftaric acid, fructose, and storage temperature on the formation of furan-derived compounds during storage of base wines. Base wines produced from Chardonnay grapes were stored at 15 and 30 °C for 90 days with additions of fructose, caffeic acid, and caftaric acid independently or in combinations. Wines were analyzed following 90 days of storage for: total hydroxycinnamic acids, degree of browning, caffeic acid and caftaric acid concentrations, and nine furan-derived compounds. Caffeic and caftaric acid additions increased homofuraneol concentration by 31% and 39%, respectively, at 15 °C (p < 0.05). Only the addition of caffeic acid increased furfural by 15% at 15 °C (p < 0.05). Results demonstrate that some furan derivatives over 90 days at 15 °C increased slightly with 5 mg/L additions of caffeic and caftaric acid. This is the first time the influence of hydroxycinnamic acids on furan-derived compounds has been reported during short-term aging of base wine at cellar temperature.
Comparative Study of Antimicrobial Activity of Silver, Gold, and Silver/Gold Bimetallic Nanoparticles Synthesized by Green Approach
Authors: Sher Naila , Alkhalifah Dalal Hussien M. , Ahmed Mushtaq , Mushtaq Nadia , Shah Faridullah , Fozia Fozia , Khan Rahmat Ali , Hozzein Wael N. , Aboul-Soud Mourad A. M.
Published: November 2022

Nanotechnology is one of the most recent technologies. It is uncertain whether the production of small-size nanoparticles (NPs) can be achieved through a simple, straightforward, and medicinally active phytochemical route. The present study aimed to develop an easy and justifiable method for the synthesis of Ag, Au, and their Ag/Au bimetallic NPs (BNPs) by using Hippeastrum hybridum (HH) extract, and then to investigate the effects of Ag, Au, and their Ag/Au BNPs as antimicrobial and phytotoxic agents. Ag, Au, and their Ag/Au BNPs were characterized by UV-visible spectroscopy, FT-IR spectroscopy, XRD, EDX, and SEM analysis. XRD analysis conferring to the face of face-centered cubic crystal structure with an average size of 13.3, 10.72, and 8.34 nm of Ag, Au, and Ag/Au BNPs, respectively. SEM showed that Ag, Au, and Ag/Au BNPs had spherical morphologies, with calculated nano measurements of 40, 30, and 20 nm, respectively. The EDX analysis confirmed the composition of elemental Ag signal of the HH-AgNPs with 22.75%, Au signal of the HH-AuNPs with 48.08%, Ag signal with 12%, and Au signal with 38.26% of the Ag/Au BNPs. The Ag/Au BNPs showed an excellent antimicrobial efficacy against Gram-positive Staphylococcus aureus, Actinomycetes meriye, Bacillus cereus, Streptococcus pyogenes, Methicillin-resistant Staphylococcus aureus, Micrococcus luteus, Streptococcus pneumonia, and Gram-negative Klebsiella pneumonia, Escherichia coli, and Serratia marcescens bacterial strains, as well as against three fungal strains (Aspergillus niger, Aspergillus fumigatus, and Aspergillus flavus) compared to HH extract, HH-AgNPs, and HH-AuNPs. However, further investigations are recommended to be able to minimize potential risks of application.
Influence of Enzymatic Hydrolysis and Molecular Weight Fractionation on the Antioxidant and Lipase / α-Amylase Inhibitory Activities In Vitro of Watermelon Seed Protein Hydrolysates
Authors: Mirzapour-Kouhdasht Armin , Garcia-Vaquero Marco , Eun Jong-Bang , Simal-Gandara Jesus
Published: November 2022

This study aims to evaluate the potential in vitro antioxidant and anti-obesity activities of watermelon seed protein hydrolysates (WSPH) obtained using different combinations of enzymes alcalase–proteinase K (ALC-PK) and alcalase–actinidin (ALC-ACT). There was a direct relationship between the degree of hydrolysis (DH) and the biological activities of the WSPH, with the highest DPPH (approximately 85%) and lipase inhibitory activities (≈59%) appreciated at DH of 36–37% and 33–35% when using ALC-PK and ALC-ACT, respectively. Following molecular weight fractionation, the ALC-PK WSPH < 3 kDa (F1) assayed at 1 mg.mL−1 had the highest DPPH-radical scavenging (89.22%), ferrous chelating (FC) (79.83%), reducing power (RP) (A 0.51), lipase inhibitory (71.36%), and α-amylase inhibitory (62.08%) activities. The amino acid analysis of ALC-PK WSPH and its fractions revealed a relationship between the biological activity of the extracts and their composition. High contents of hydrophobic amino acids, arginine, and aromatic amino acids were related to high antioxidant, lipase inhibitory, and α-amylase inhibitory activities in the extracts, respectively. Overall, this study revealed that underutilized protein sources such as WSPH, using the appropriate combination of enzymes, could result in the generation of new ingredients and compounds with powerful antioxidant and anti-obesity activities with promising applications as nutraceuticals or functional foods.
Achillea millefolium Essential Oil Mitigates Peptic Ulcer in Rats through Nrf2/HO-1 Pathway
Authors: Alomair Manar K. , Alabduladheem Lama S. , Almajed Marwah A. , Alobaid Amjad A. , Alkhalifah Essraa A. R. , Younis Nancy S. , Mohamed Maged E.
Published: November 2022

Extreme ethanol ingestion is associated with developing gastric ulcers. Achillea millefolium (yarrow) is one of the most commonly used herbs with numerous proven pharmacological actions. The goal of the hereby investigation is to explore the gastroprotective action of yarrow essential oil against ethanol-induced gastric ulcers and to reveal the unexplored mechanisms. Rats were distributed into five groups (n = 6); the control group administered 10% Tween 20, orally, for two weeks; the ethanol group administered absolute ethanol (5 mL/kg) to prompt gastric ulcer on the last day of the experiment. Yarrow essential oil 100 or 200 mg/kg + ethanol groups pretreated with yarrow oil (100 or 200 mg/kg, respectively), orally, for two weeks prior to gastric ulcer induction by absolute ethanol. Lanso + ethanol group administered 20 mg/kg lansoprazole, orally, for two weeks prior to gastric ulcer induction by ethanol. Results of the current study showed that ethanol caused several macroscopic and microscopic alterations, amplified lipid peroxidation, pro-inflammatory cytokines, and apoptotic markers, as well as diminished PGE2, NO, and antioxidant enzyme activities. On the other hand, animals pretreated with yarrow essential oil exhibited fewer macroscopic and microscopic modifications, reduced ulcer surface, and increased Alcian blue binding capacity, pH, and pepsin activity. In addition, yarrow essential oil groups exhibited reduced pro-inflammatory cytokines, apoptotic markers, and MDA, restored the PGE2 and NO levels, and recovered the antioxidant enzyme activities. Ethanol escalated Nrf2 and HO-1 expressions, whereas pretreatment of yarrow essential oil caused further intensification in Nrf2 and HO-1. To conclude, the current study suggested yarrow essential oil as a gastroprotective agent against ethanol-induced gastric lesions. This gastroprotective effect could be related to the antioxidant, anti-inflammatory, and anti-apoptotic actions of the essential oil through the instigation of the Nrf2/HO-1 pathway.
Structural Basis for (2R,3R)-Taxifolin Binding and Reaction Products to the Bacterial Chalcone Isomerase of Eubacterium ramulus
Authors: Palm Gottfried J. , Thomsen Maren , Berndt Leona , Hinrichs Winfried
Published: November 2022

The bacterial chalcone isomerase (CHI) from Eubacterium ramulus catalyses the first step in a flavanone-degradation pathway by a reverse Michael addition. The overall fold and the constitution of the active site of the enzyme completely differ from the well-characterised chalcone isomerase of plants. For (+)-taxifolin, CHI catalyses the intramolecular ring contraction to alphitonin. In this study, Fwe perform crystal structure analyses of CHI and its active site mutant His33Ala in the presence of the substrate taxifolin at 2.15 and 2.8 Å resolution, respectively. The inactive enzyme binds the substrate (+)-taxifolin as well defined, whereas the electron density maps of the native CHI show a superposition of substrate, product alphitonin, and most probably also the reaction intermediate taxifolin chalcone. Evidently, His33 mediates the stereospecific acid-base reaction by abstracting a proton from the flavonoid scaffold. The stereospecificity of the product is discussed.
Wild Boar (Sus scrofa)—Fascioloides magna Interaction from the Perspective of the MHC Genes
Authors: Konjević Dean , Erman Vlatka , Bujanić Miljenko , Svetličić Ida , Arbanasić Haidi , Lubura Strunjak Snježana , Galov Ana
Published: November 2022

Fascioloidosis is a parasitic disease caused by a trematode Fascioloides magna. Since major histocompatibility complex (MHC) genes play an important role in the immune response, the aim of this study was to compare the potential differences in MHC class II SLA-DRB1 exon 2 genes between wild boar populations from infected (cases) and non-infected areas (controls). During the winter of 2021, a total of 136 wild boar tissue samples were collected, 39 cases and 97 controls. DNA was extracted and sequenced using the Illumina platform. Differences in distributions of allele combinations were calculated using the Chi-Square test for homogeneity and between proportions using the large-sample test and Fisher–Irwin test. Analysis revealed 19 previously described swine leucocyte antigen (SLA) alleles. The number of polymorphic sites was 79 (29.6%), with 99 mutations in total. Nucleotide diversity π was estimated at 0.11. Proportions of the alleles SLA-DRB1*12:05 (p = 0.0008379) and SLA-DRB1*0101 (p = 0.0002825) were statistically significantly higher in controls, and proportions of the SLA-DRB1*0602 (p = 0.006059) and SLA-DRB1*0901 (p = 0.0006601) in cases. Alleles SLA-DRB1*04:09, SLA-DRB1*0501, SLA-DRB1*11:09, and SLA-DRB1*1301 were detected only in cases, while SLA-DRB1*0404, SLA-DRB1*0701, SLA-DRB1*02:10, and SLA-DRB1*04:08 were present only in controls. We did not confirm the existence of specific alleles that could be linked to F. magna infection. Detected high variability of the MHC class II SLA-DRB1 exon 2 genes indicate high resistance potential against various pathogens.
Molecular Mimicry Analyses Unveiled the Human Herpes Simplex and Poxvirus Epitopes as Possible Candidates to Incite Autoimmunity
Authors: Begum Sara , Aiman Sara , Ahmad Shujaat , Samad Abdus , Almehmadi Mazen , Allahyani Mamdouh , Aljuaid Abdulelah , Afridi Sahib Gul , Khan Asifullah
Published: November 2022

Clinical epidemiological studies have reported that viral infections cause autoimmune pathology in humans. Host-pathogen protein sequences and structure-based molecular mimicry cause autoreactive T cells to cross-activate. The aim of the current study was to implement immunoinformatics approaches to infer sequence- and structure-based molecular mimicry between viral and human proteomic datasets. The protein sequences of all the so far known human-infecting viruses were obtained from the VIPR database, and complete human proteome data were retrieved from the NCBI repository. Based on a predefined, stringent threshold of comparative sequence analyses, 24 viral proteins were identified with significant sequence similarity to human proteins. PathDIP identified the enrichment of these homologous proteins in nine metabolic pathways with a p-value < 0.0001. Several viral and human mimic epitopes from these homologous proteins were predicted as strong binders of human HLA alleles, with IC50 < 50 nM. Downstream molecular docking analyses identified that lead virus-human homologous epitopes feasibly interact with HLA and TLR4 types of immune receptors. The vast majority of these top-hit homolog epitopic peptides belong to the herpes simplex and poxvirus families. These lead epitope biological sequences and 3D structural-based molecular mimicry may be promising for interpreting herpes simplex virus and poxvirus infection-mediated autoimmune disorders in humans.
Integrated Multitrophic Aquaculture; Analysing Contributions of Different Biological Compartments to Nutrient Removal in a Duckweed-Based Water Remediation System
Authors: Paolacci Simona , Stejskal Vlastimil , Toner Damien , Jansen Marcel A. K.
Published: November 2022

Duckweed (Lemnaceae) can support the development of freshwater aquaculture if used as extractive species in Integrated MultiTrophic Aquaculture (IMTA) systems. These aquatic plants have the advantage of producing protein-rich biomass that has several potential uses. On the contrary, other biological compartments, such as microalgae and bacteria, present in the water and competing with duckweed for light and nutrients cannot be harvested easily from the water. Moreover, as phytoplankton cannot easily be harvested, nutrients are eventually re-released; hence, this compartment does not contribute to the overall water remediation process. In the present study, a mesocosm experiment was designed to quantify the portion of nutrients effectively removed by duckweed in a duckweed-based aquaculture wastewater remediation system. Three tanks were buried next to a pilot-scale IMTA system used for the production of rainbow trout and perch. The tanks received aquaculture effluents from the adjacent system, and 50% of their surface was covered by duckweed. Daily water analyses of samples at the inlet and outlet of the mesocosm allowed quantification of the amount of nutrients removed in total. The portion removed by duckweed was determined by examining the nutrient content in the initial and final biomass. The portion of nutrients removed by other compartments was similarly estimated. The results show that duckweed is responsible for the removal of 31% and 29% of N and P, respectively. Phytoplankton removed 33% and 38% of N and P, respectively, while the biofilm played no major role in nutrient removal. The remainder of the removed nutrients were probably assimilated by bacteria or sedimented. It is speculated that a higher initial duckweed density can limit phytoplankton growth and, therefore, increase the portion of nutrients removed by the duckweed compartment.
Photosynthetic Traits of Páramo Plants Subjected to Short-Term Warming in OTC Chambers
Authors: Solarte María Elena , Solarte Erazo Yisela , Ramírez Cupacán Elizabeth , Enríquez Paz Camila , Melgarejo Luz Marina , Lasso Eloisa , Flexas Jaume , Gulias Javier
Published: November 2022

Global warming and changes in land use are some of the main threats to high mountain species. Both can interact in ways not yet assessed. In this study, we evaluated the photosynthetic responses of six common páramo species within a warming experiment using open-top chambers (OTC) in conserved páramo areas with different land use histories. We did not find significant differences in the photochemical performance of the species as measured through Fv/Fm, ETR, and NPQ in response to passive warming, indicating that warmed plants are not stressed. However, NPQ values were higher in recovering areas, especially in the driest and warmest months. Leaf transpiration, stomatal conductance, and Ci were not affected by the OTC or the land use history. The photosynthetic capacity, maximum photosynthetic capacity, and carboxylation rate of RuBisCO increased in response to warming but only in the area with no anthropogenic intervention. These results suggest that species will respond differently to warming depending on the history of páramo use, and therefore not all páramo communities will respond equally to climate change. In disturbed sites with altered soil conditions, plants could have a lower breadth of physiological response to warming.
Fruit Morphology and Ripening-Related QTLs in a Newly Developed Introgression Line Collection of the Elite Varieties ‘Védrantais’ and ‘Piel de Sapo’
Authors: Santo Domingo Miguel , Mayobre Carlos , Pereira Lara , Argyris Jason , Valverde Laura , Martín-Hernández Ana Montserrat , Garcia-Mas Jordi , Pujol Marta
Published: November 2022

Melon is an economically important crop with widely diverse fruit morphology and ripening characteristics. Its diploid sequenced genome and multiple genomic tools make this species suitable to study the genetic architecture of fruit traits. With the development of this introgression line population of the elite varieties ‘Piel de Sapo’ and ‘Védrantais’, we present a powerful tool to study fruit morphology and ripening traits that can also facilitate characterization or pyramidation of QTLs in inodorous melon types. The population consists of 36 lines covering almost 98% of the melon genome, with an average of three introgressions per chromosome and segregating for multiple fruit traits: morphology, ripening and quality. High variability in fruit morphology was found within the population, with 24 QTLs affecting six different traits, confirming previously reported QTLs and two newly detected QTLs, FLQW5.1 and FWQW7.1. We detected 20 QTLs affecting fruit ripening traits, six of them reported for the first time, two affecting the timing of yellowing of the rind (EYELLQW1.1 and EYELLQW8.1) and four at the end of chromosome 8 affecting aroma, abscission and harvest date (EAROQW8.3, EALFQW8.3, ABSQW8.3 and HARQW8.3). We also confirmed the location of several QTLs, such as fruit-quality-related QTLs affecting rind and flesh appearance and flesh firmness.
High-Dose Assessment of Transgenic Insect-Resistant Maize Events against Major Lepidopteran Pests in China
Authors: Li Guoping , Ji Tingjie , Zhao Shengyuan , Feng Hongqiang , Wu Kongming
Published: November 2022

Lepidopteran pests present a key problem for maize production in China. In order to develop a new strategy for the pest control, the Chinese government has issued safety certificates for insect-resistant transgenic maize, but whether these transformation events can achieve high dose levels to major target pests is still unclear. In this paper, the transformation events of DBN9936 (Bt-Cry1Ab), DBN9936 × DBN9501 (Bt-Cry1Ab + Vip3A), Ruifeng 125 (Bt-Cry1Ab/Cry2Aj), and MIR162 (Bt-Vip3A) were planted in the Huang-huai-hai summer corn region of China to evaluate the lethal effects on major lepidopteran pests, Spodoptera frugiperda, Helicoverpa armigera, Ostrinia furnacalis, Conogethes punctiferalis, Mythimna separata, Leucania loreyi, and Athetis lepigone, using an artificial diet containing lyophilized Bt maize tissue at a concentration representing a 25-fold dilution of tissue. The results showed that the corrected mortalities of DBN9936 (Bt-Cry1Ab), DBN9936 × DBN9501 (Bt-Cry1Ab + Vip3A), Ruifeng 125 (Bt-Cry1Ab/Cry2Aj), and MIR162 (Bt-Vip3A) to the seven pests were in the ranges 53.80~100%, 62.98~100%, 57.09~100%, and 41.02~100%, respectively. In summary, the events of DBN9936, DBN9936 × DBN9501, and MIR162 reached high dose levels to S. frugiperda. DBN9936 × DBN9501 only at the R1 stage reached a high dose level to H. armigera. DBN9936, DBN9936 × DBN9501, and Ruifeng 125, at most growth stages, reached high dose levels to O. furnacalis, and these three events at some stages also reached high dose levels to A. lepigone. Ruifeng 125 presented a high dose level only to C. punctiferalis. However, no transformations reached high dose levels to either M. separata or L. loreyi. This study provides a support for the breeding of high-dose varieties to different target pests, the combined application of multiple genes and the commercial regional planting of insect-resistant transgenic maize in China.
FABRIKx: Tackling the Inverse Kinematics Problem of Continuum Robots with Variable Curvature
Authors: Kolpashchikov Dmitrii , Gerget Olga , Danilov Viacheslav
Published: November 2022

A continuum robot is a unique type of robots which move because of the elastic deformation of their bodies. The kinematics of such robots is typically described using constant curvature assumption. Such an assumption, however, does not completely describe the kinematics of a real-life continuum robot. As a result, variable curvature assumptions describe the kinematics of the continuum robot better, however, they are more complicated to formulate and work with. In particular, the existing methods of solving the inverse kinematics problem of multisection continuum robots with variable curvature suffer from a variety of deficiencies. Those deficiencies include complex matrix calculations, singularity problems, unscalability, and inability to find a numeric solution in some cases. In this work, we present FABRIKx: fast and reliable algorithm to solve the problem of inverse kinematics of the multisection continuum robot with variable curvature. In particular, to describe the variable curvature, we utilize a piecewise constant curvature assumption. The proposed algorithm combines both tangent and chord approaches to solve the inverse kinematics problem. The inverse kinematics of a single bending section of piecewise constant curvature is also described. To evaluate FABRIKx effectiveness, we compare it with the Jacobian-based and FABRIKc-based algorithms via simulation studies for different robots. The obtained results show that FABRIKx demonstrates a higher success rate and a lower solution time.
LRF-WiVi: A WiFi and Visual Indoor Localization Method Based on Low-Rank Fusion
Authors: Liu Wen , Qin Changyan , Deng Zhongliang , Jiang Haoyue
Published: November 2022

In this paper, a WiFi and visual fingerprint localization model based on low-rank fusion (LRF-WiVi) is proposed, which makes full use of the complementarity of heterogeneous signals by modeling both the signal-specific actions and interaction of location information in the two signals end-to-end. Firstly, two feature extraction subnetworks are designed to extract the feature vectors containing location information of WiFi channel state information (CSI) and multi-directional visual images respectively. Then, the low-rank fusion module efficiently aggregates the specific actions and interactions of the two feature vectors while maintaining low computational complexity. The fusion features obtained are used for position estimation; In addition, for the CSI feature extraction subnetwork, we designed a novel construction method of CSI time-frequency characteristic map and a double-branch CNN structure to extract features. LRF-WiVi jointly learns the parameters of each module under the guidance of the same loss function, making the whole model more consistent with the goal of fusion localization. Extensive experiments are conducted in a complex laboratory and an open hall to verify the superior performance of LRF-WiVi in utilizing WiFi and visual signal complementarity. The results show that our method achieves more advanced positioning performance than other methods in both scenarios.
Safety Study of High-Speed Collisions between Trains and Live Intruder
Authors: Zhang Hai , Fu Gengzhe , Su Yongzhang , Yue Yixin , Zhu Wei , Zhang Chenyu , Lu Yuxiang
Published: November 2022

To investigate the safety of train collisions with live intruders under high-speed operation, a new 3D finite element laminated model of live intruder filling was constructed based on reconstruction using physical 3D scanning, with three outer layers of the model simulating the skin, three inner layers simulating bone, and internal filling simulating internal organs. The model was simulated in LS-DYNA with pendulum side collision, and the force–time and force–displacement curves of the collision between the pendulum and the living intruder were obtained, which were consistent with the curve trend of the results of the cadaver pendulum collision test by Viano in 1989, and the accuracy of the finite element model of the intruder was verified. Through the simulation calculation of high-speed collision between the train and two kinds of living intrusions, the maximum acceleration of the train body, the maximum lifting of the wheel pair, the deformation of the cowcatcher, and the maximum central load on the cowcatcher during the collision can be obtained. The results of the study show that at a collision speed of 110 km/h and different collision positions, the collision risk factor between the train and heavier organisms is relatively high, and the risk arising from frontal collisions is generally greater than that of offset collisions; despite this, all the indicators such as the maximum acceleration of the train, the maximum lift of the wheel pairs, the reduction in the length of the cowcatcher discharge per 5 m of space, and the maximum central load borne by the cowcatcher discharge are lower than the EN15227 standard. Additionally, the safety of the train is not affected and the components can work reliably.
Neural Network-Based Autonomous Search Model with Undulatory Locomotion Inspired by Caenorhabditis Elegans
Authors: Chen Mohan , Feng Dazheng , Su Hongtao , Wang Meng , Su Tingting
Published: November 2022

Caenorhabditis elegans (C. elegans) exhibits sophisticated chemotaxis behavior with a unique locomotion pattern using a simple nervous system only and is, therefore, well suited to inspire simple, cost-effective robotic navigation schemes. Chemotaxis in C. elegans involves two complementary strategies: klinokinesis, which allows reorientation by sharp turns when moving away from targets; and klinotaxis, which gradually adjusts the direction of motion toward the preferred side throughout the movement. In this study, we developed an autonomous search model with undulatory locomotion that combines these two C. elegans chemotaxis strategies with its body undulatory locomotion. To search for peaks in environmental variables such as chemical concentrations and radiation in directions close to the steepest gradients, only one sensor is needed. To develop our model, we first evolved a central pattern generator and designed a minimal network unit with proprioceptive feedback to encode and propagate rhythmic signals; hence, we realized realistic undulatory locomotion. We then constructed adaptive sensory neuron models following real electrophysiological characteristics and incorporated a state-dependent gating mechanism, enabling the model to execute the two orientation strategies simultaneously according to information from a single sensor. Simulation results verified the effectiveness, superiority, and realness of the model. Our simply structured model exploits multiple biological mechanisms to search for the shortest-path concentration peak over a wide range of gradients and can serve as a theoretical prototype for worm-like navigation robots.
Control Strategy of an Underactuated Underwater Drone-Shape Robot for Grasping Tasks
Authors: Cely Juan S. , Pérez Bayas Miguel Ángel , Carpio Marco , García Cena Cecilia Elisabet , Sintov Avishai , Saltaren Roque
Published: November 2022

In underwater environments, ensuring people’s safety is complicated, with potentially life-threatening outcomes, especially when divers have to work in deeper conditions. To improve the available solutions for working with robots in this kind of environment, we propose the validation of a control strategy for robots when taking objects from the seabed. The control strategy proposed is based on acceleration feedback in the model of the system. Using this model, the reference values for position, velocity and acceleration are estimated, and then the position error signal can be computed. When the desired position is obtained, it is possible to then obtain the position error. The validation was carried out using three different objects: a ball, a bottle, and a plant. The experiment consisted of using this control strategy to take those objects, which the robot carried for a moment to validate the stabilisation control and reference following the control in terms of angle and depth. The robot was operated by a pilot from outside of the pool and was guided using a camera and sonar in a teleoperated way. As an advantage of this control strategy, the model upon which the robot is based is decoupled, allowing control of the robot for each uncoupled plane, this being the main finding of these tests. This demonstrates that the robot can be controlled by a control strategy based on a decoupled model, taking into account the hydrodynamic parameters of the robot.
Inertial Indoor Pedestrian Navigation Based on Cascade Filtering Integrated INS/Map Information
Authors: Fan Menghao , Li Jia , Wang Weibing
Published: November 2022

Indoor pedestrian positioning has been widely used in many scenarios, such as fire rescue and indoor path planning. Compared with other technologies, inertial measurement unit (IMU)-based indoor positioning requires no additional equipment and has a lower cost. However, IMU-based indoor positioning has the problem of error accumulation, resulting in inaccurate positioning. Therefore, this paper proposes a cascade filtering algorithm to correct the accumulated error using only a small amount of map information. In the lower filter, the zero-velocity correction and the attitude-extended complementary filtering (ECF) algorithm are utilized to initially solve the pedestrian’s trajectory. In the upper filter, a particle filter (PF) combined with the map information is adopted to correct the accumulated error of the heading and stride length. In the 2D positioning process, the root mean square error (RMSE) of the proposed algorithm is only 1.35 m. In the altitude correction, this paper proposes a method of clustering floor discrimination to deal with the instability of the barometer resulting from an uneven pressure and temperature. In the final 3D positioning experiment, with a total length of 536.5 m and including the process of going up and down the stairs, the end-point error is only 2.45 m by the proposed algorithm.
Supervised Object-Specific Distance Estimation from Monocular Images for Autonomous Driving
Authors: Davydov Yury , Chen Wen-Hui , Lin Yu-Chen
Published: November 2022

Accurate distance estimation is a requirement for advanced driver assistance systems (ADAS) to provide drivers with safety-related functions such as adaptive cruise control and collision avoidance. Radars and lidars can be used for providing distance information; however, they are either expensive or provide poor object information compared to image sensors. In this study, we propose a lightweight convolutional deep learning model that can extract object-specific distance information from monocular images. We explore a variety of training and five structural settings of the model and conduct various tests on the KITTI dataset for evaluating seven different road agents, namely, person, bicycle, car, motorcycle, bus, train, and truck. Additionally, in all experiments, a comparison with the Monodepth2 model is carried out. Experimental results show that the proposed model outperforms Monodepth2 by 15% in terms of the average weighted mean absolute error (MAE).
Adaptive Quality of Service Control for MQTT-SN
Authors: Palmese Fabio , Redondi Alessandro E. C. , Cesana Matteo
Published: November 2022

Internet of Things and wireless sensor network applications are becoming more and more popular these days, supported by new communication technologies and protocols tailored to their specific requirements. This paper focuses on improving the performance of a Wireless Sensor Network operated by the MQTT-SN protocol, one of the most popular publish/subscribe protocols for IoT applications. In particular, we propose a dynamic Quality of Service (QoS) controller for the MQTT-SN protocol, capable of evaluating the status of the underlying network in terms of end-to-end delay and packet error rate, reacting consequently by assigning the best QoS value to a node. We design and implement the QoS controller in a simulated environment based on the ns-3 network emulator, and we perform extensive experiments to prove its effectiveness compared to a non-controlled scenario. The reported results show that, by controlling the quality of service, it is possible to effectively manage the number of packets successfully received by each device and their average latency, to improve the quality of the communication of each end node.
Cesium Lead Bromide Nanocrystals: Synthesis, Modification, and Application to O2 Sensing
Authors: Huang Zhi-Hao , Layek Madhuja , Li Chia-Feng , Lee Kun-Mu , Huang Yu-Ching
Published: November 2022

The fluorescence intensity of inorganic CsPbBr3 (CPB) perovskite nanocrystals (NCs) decreases in the presence of O2. In this study, we synthesized CPB NCs with various shapes and sizes for use as optical gas sensing materials. We fabricated O2 gas sensors from the various CPB NCs on several porous and nonporous substrates and examined the effects of the NC shapes and aggregate sizes and the substrate pore size on the device response. Our sensor fabricated from CPB nanocrystals on a porous substrate exhibited the highest response; the porous substrate allowed the rapid diffusion of O2 such that the NC surface was exposed effectively to the gas. Thus, the interfacial interaction between NC surfaces and substrates is a critical factor for consideration when preparing gas sensors with a high response.
Modernization of higher education academic programs at the Faculty of Pharmacy, Sechenov First Moscow State Medical University, in the period from 2013/2014 to 2017/2018 academic years
Authors:   Moscow Medical University «REAVIZ», Golikova N. S.
Published: November 2022

In the period of 2015–2018, a comprehensive medical and sociological study of the compliance of the professional training of pharmacists graduating from Moscow higher educational institutions with the requirements of regional pharmaceutical enterprises was carried out. As a result of the questionnaire surveys and a series of expert interviews, critically important skills, abilities, and knowledge necessary for young professionals were identified. The results of the study, analyzed and implemented in the practice of the Sechenov First Moscow State Medical University of the Ministry of Health of Russia as changes in the academic programs of the Faculty of Pharmacy, are presented in this article.
O discurso anticomunista no tempo presente: entre continuidades e descontinuidades (2013-2018)
Authors:   , Oliveira Talles de Almeida
Published: November 2022

Esta pesquisa tem como objetivo investigar o recrudescimento de discursos anticomunistas na esfera pública brasileira, mobilizados entre os anos de 2013 e 2018. Por meio da análise de petições públicas (plataforma Change.org) e propostas legislativas (plataforma Ideias Legislativas) e da seção destinada às cartas de leitores dos jornais Folha de São Paulo e O Globo, procuramos examinar os discursos utilizados por sujeitos portadores de retóricas anticomunistas para se oporem àquilo que identificam como a ideologia e a prática comunistas. Partimos da hipótese mais geral segundo a qual o anticomunismo brasileiro do tempo presente é constituído de continuidades e descontinuidades em relação ao anticomunismo de outros momentos da história do país. Assim, entendemos o anticomunismo enquanto um processo, que carrega elementos estruturais próprios dos mitos políticos e aspectos contingenciais, sujeitos às dinâmicas políticas e sociais de cada conjuntura específica.
Perfil dos custos ambulatoriais no tratamento de neoplasia prostática
Authors:   , Oliveira Adriana Elisa Carcereri de
Published: November 2022

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A organização de tempos e espaços para o brincar na educação infantil numa escola privada da cidade de Juiz de Fora
Authors:   , Fernandes Paula Raquel da Costa
Published: November 2022

A presente dissertação foi desenvolvida no âmbito do Mestrado Profissional em Gestão e Avaliação da Educação do Centro de Políticas Públicas e Avaliação da Educação da Universidade Federal de Juiz de Fora (CAEd/UFJF). O caso de gestão estudado discute e busca compreender caminhos que potencializem o brincar como atividade estruturante das experiências na educação infantil de uma escola privada do município de Juiz de Fora. Assumimos como hipóteses que os tempos e espaços destinados ao brincar livre podem ser utilizados com maior qualidade, potencializando o desenvolvimento infantil por meio da atividade de brincar no parque. Neste trabalho, utilizamos como metodologia, pesquisa bibliográfica e o estudo de caso e como instrumento de pesquisa o grupo focal. Tendo isso em vista, fizemos um percurso a partir de um resgate das Diretrizes Curriculares Nacionais da Educação Infantil (DCNEI), em seu art. 9º, que apontam como eixos estruturantes das práticas pedagógicas dessa etapa da Educação Básica as interações e a brincadeira - experiências nas quais as crianças podem construir e apropriar-se de conhecimentos por meio de suas ações e interações com seus pares e com os adultos, o que possibilita aprendizagens, desenvolvimento e socialização (BRASIL, 2009). Recorremos também ao trecho da Base Nacional Comum Curricular (BNCC), que é atravessada pela concepção que o desenvolvimento infantil se dá durante a interação entre sujeitos na realização da atividade de brincar. Nesse sentido, pudemos investigar - à luz de uma visão respaldada pela perspectiva histórico-cultural, preconizada principalmente por Vygotsky, que teve como objetivo geral a reflexão acerca dos tempos e espaços de qualidade para o brincar - como a gestão pode promover a prática do brincar como atividade estruturante das experiências na Educação Infantil. Por meio dos resultados obtidos nesta pesquisa, pudemos perceber que existem não apenas lacunas, como um grande e diversificado repertório de possibilidades que vislumbram o brincar como atividade principal no desenvolvimento das crianças da educação infantil. Foi elaborado um Plano de Ação Educacional, com enfoque no melhor aproveitamento das práticas pedagógicas atreladas ao brincar, principalmente no espaço do parquinho, sendo a equipe gestora a grande mediadora de todo esse processo. As ações propostas por este estudo sugerem possibilidades para a formação continuada dos profissionais na própria instituição, por meio de estudos, discussões, seminários, palestras direcionadas a todos os envolvidos no processo de aprendizagem dos alunos, que possam incluir sugestões de atividades e relatos de experiências exitosas que colaborem com a inserção do brincar como prática cotidiana da instituição em questão.