bluuebunny/crossref_metadata_2025 huggingface dataset:
Basic Skills in Higher Education: An Analysis of Attributed Importance
Authors: Aranda Lourdes , Mena-Rodríguez Esther , Rubio Laura
Published: February 2022

Today, the skills-based approach is increasingly in demand by companies due, in large part, to the fact that it favors the management of human resources by focusing on individual capabilities; which, finally, improves the job profile of a company. As a result, choosing the right candidates has become increasingly selective. Universities, therefore, need to teach skills to improve the incorporation of graduates into the workplace making it as successful as possible. For this reason, it is of special relevance to know if college students consider that the acquisition of skills is key for their incorporation into the workplace. The main objective of this study was to analyze and compare the importance assigned to the acquisition of basic skills in the university education of 694 students studying four different bachelor degrees: pedagogy, early childhood education, primary education, and psychology. For this purpose, a Likert-type questionnaire on basic skills was distributed with four possible options and the following five dimensions that grouped basic skills: organizational and planning capacity; access to information sources; analysis and synthesis of texts, situations, and people; teamwork; and problem solving. The results show that as a whole all students across different bachelor degrees gave a high score to the acquisition of basic skills, with early childhood education students giving it greater importance compared to the students from other disciplines and, more specifically, differences were observed in some dimensions depending on the bachelor degree that they have started.
Working Online During COVID-19: Accounts of First Year Students Experiences and Well-Being
Authors: Moosa Moeniera , Bekker Tanya
Published: February 2022

The sudden move to online learning due to the COVID-19 pandemic has created an influx of epistemological, psycho-social, emotional and financial challenges for first year students. Lecturers and academics had to find creative and sustainable ways of ensuring that all students were epistemologically included. New policies and practices were introduced rapidly at universities to facilitate the unavoidable move to online learning. As initial teacher educators at a public University in South Africa we noted that the sudden move to working online has presented various challenges to first year students’ overall well-being which has further exacerbated issues of exclusion and marginalization for many. We argue that it is against this backdrop that this paper explores how the move to online learning has affected first year students’ overall well-being, at one teacher education institution. The Index for Inclusive Education was used as a theoretical lens to explore student’s perceptions of the institution’s policy, teaching and learning practices, and the institutional culture during this period. One hundred and eighty-seven purposively selected first year students participated in this qualitative, phenomenological research study. Data were collected by means of open-ended questionnaires. Responses were categorized by means of an emergent thematic analysis. The findings indicated that online learning compromised various aspects of well-being including physical, emotional, psycho-social and financial well-being for many students. The experiences of online learning and impact on well-being did, however, differ across students depending on their individual contexts and circumstances indicating that considerations of well-being need to take contextual realities into account to support the well-being and learning of all. We recommend that higher education institutions prioritize the psycho-social, emotional, and financial well-being of students during the period of online learning and not just the pedagogic needs of the qualification.
Group Membership Modulates Fairness Consideration Among Deaf College Students—An Event-Related Potential Study
Authors: Gong Yuqi , Yao Li , Chen Xiaoyi , Xia Qingling , Jiang Jun , Du Xue
Published: February 2022

Group interaction is an essential way of social interaction and plays an important role in our social development. It has been found that when individuals participate in group interactions, the group identity of the interaction partner affects the mental processing and behavioral decision-making of subjects. However, little is known about how deaf college students, who are labeled distinctly different from normal hearing college students, will react when facing proposers from different groups in the ultimatum game (UG) and its time course. In this study, we recruited 29 deaf college students who played the UG in which they received extremely unfair, moderately unfair, or fair offers from either outgroup members (normal hearing college students) or ingroup members (deaf college students), while their brain potentials were recorded. The behavioral results showed that group membership did not impact the acceptance rate of deaf college students. But, event-related potential (ERP) analysis demonstrated an enhanced feedback-related negativity (FRN) elicited by ingroup members compared to outgroup members. Importantly, we found that under fairness conditions, deaf college students induced more positive P2 and P3 facing ingroup members compared to outgroup members. Our results demonstrated that group membership may modulate the performance of deaf college students in the UG and the existence of ingroup bias among deaf college students. This provides some evidence for the fairness characteristics of special populations, so that to improve the educational integration of colleges and universities.
Fathers’ Sensitivity in Infancy and Externalizing Problems in Middle Childhood: The Role of Coparenting
Authors: Jacobvitz Deborah , Aviles Ashleigh I. , Aquino Gabriela A. , Tian Ziyu , Zhang Shuqi , Hazen Nancy
Published: February 2022

The present study examined the role of father sensitivity and couple coparenting quality in the first 2 years of life in relation to the development of externalizing behavior problems in middle childhood, focusing on the unique role of fathers. In this study, 125 mothers, fathers, and their first-born children were followed from 8 months to age 7 years. Paternal sensitivity was rated when infants were 8 and 24 months old. Fathers were videotaped at home playing, feeding, and changing their 8-month-old infants’ clothes. They also were videotaped in a lab playing with their 24-month-olds and solving a variety of challenging tasks. At 24 months, competitive coparenting was assessedviavideotaped triadic family interactions at home in which families participated in a variety of tasks (i.e., clothes change, eating a snack together and solving tasks). Teachers rated externalizing behavior problems when the children were age 7. Continuity in paternal sensitivity was documented from 8 to 24 months, and paternal sensitivity at 8 months predicted externalizing behavior in middle childhood through father sensitivity at 24 months. Moreover, paternal sensitivity at 8 months predicted competitive coparenting which, in turn, forecast externalizing behavior problems in middle childhood, even after controlling for maternal sensitivity at 8 and 24 months. These findings highlight the unique role of paternal caregiving quality during the first year of life on couple coparenting and children’s subsequent development of externalizing problems and have implications for creating effective interventions to prevent children from developing externalizing disorders.
Cell-Type Specific Deletion of CB2 Cannabinoid Receptors in Dopamine Neurons Induced Hyperactivity Phenotype: Possible Relevance to Attention-Deficit Hyperactivity Disorder
Authors: Canseco-Alba Ana , Sanabria Branden , Hammouda Mariam , Bernadin Rollanda , Mina Marizel , Liu Qing-Rong , Onaivi Emmanuel S.
Published: February 2022

DAT-Cnr2 mice are conditional knockout (cKO) animals that do not express cannabinoid CB2 receptors (CB2R), in midbrain dopamine neurons. The hyperactivity phenotype of DAT-Cnr2 cKO mice were paradoxically reduced by low dose of amphetamine. Here, we report on the locomotor activity analysis in male and female adolescent (PND 30 ± 2) mice in basal conditions and in response to different doses of amphetamine, using the Open Field (OF), Elevated Plus-Maze (EPM) tests and the Novel Object Recognition (NOR) task as a putative model of attention deficit hyperactivity disorder (ADHD). Results showed that both male and female adolescent DAT-Cnr2 mice displayed significant increases in distance traveled in the OF test compared with WT mice. However, 2 mg/kg dose of amphetamine reduced the distance traveled by the DAT-Cnr2 but was increased in the WT mice. In the EPM test of anxiety-like behavioral responses, DAT-Cnr2 spent more time in the open arms of the maze than the WT mice, suggesting a reduction in anxiety-like response. DAT-Cnr2 mice showed significant increase in the number of unprotected head dips in the maze test and in the cliff avoidance reaction (CAR) test demonstrating impulsivity and risky behavior. DAT-Cnr2 mice also exhibited deficient response in the delay decision making (DDM), with impulsive choice. Both DAT-Cnr2 and WT were able to recognize the new object in the NOR task, but the exploration by the DAT-Cnr2 was less than that of the WT mice. Following the administration of 2 mg/kg of amphetamine, the similarities and differential performances of the DAT-Cnr2 and WT mice in the EPM test and NOR task was probably due to increase in attention. Microglia activation detected by Cd11b immunolabelling was enhanced in the hippocampus in DAT-Cnr2 cKO than in WT mice, implicating neuro-immune modulatory effects of CB2R. The results demonstrates that DAT-Cnr2 cKO mice with cell-type specific deletion of CB2R in midbrain dopaminergic neurons may represent a possible model for studying the neurobiological basis of ADHD.
Impact of Systematic Tailored Assessment for Responding to Suicidality (STARS) Protocol Training on Mental Health Professionals' Attitudes, Perceived Capabilities, Knowledge, and Reluctance to Intervene
Authors: Hawgood Jacinta , Ownsworth Tamara , Kõlves Kairi , Spence Susan H. , Arensman Ella , De Leo Diego
Published: February 2022

Background and Aims

Systematic Tailored Assessment for Responding to Suicidality (STARS) protocol and associated training were developed with the key objectives of supporting clinicians to conduct a suicide enquiry, obtaining a comprehensive account of psycho-social factors contributing to suicidality, and collaboratively developing a safety plan with clients. STARS training aims to address knowledge, attitudes and capabilities that influence intervention behavior/skills. This study aimed to examine associations between clinician characteristics and pre-training competencies in suicide risk assessment (SRA), as well as the impact of STARS training workshop on clinician competencies; and to determine the predictors of SRA training outcomes.

Method

Australian mental health professionals working with suicidal persons who undertook the STARS 2-day face-to-face workshop between 2018 and 2020 completed an online survey at pre- and post-training. Of the 222 participants who completed the pre-training questionnaire, 144 (64.9%) also completed the post-training questionnaire. Participants were mostly female (75.7%), had completed a university degree (86.4%), had <10 years of experience in suicide prevention (71.7%), and were allied and mental health professionals (78.1%). We used linear mixed-effects regression for statistical analyses.

Results

STARS participants who reported higher perceived capability at baseline had significantly greater formal and informal training, more years of experience in suicide prevention, and were more likely to have experienced client suicide and/or suicide attempt and to report fewer SRA related fears. We found overall significant positive impacts of STARS training on clinician competencies (attitudes, perceived capability, declarative knowledge) from pre- to post-training. The most distinct changes following STARS training were for perceived capability and declarative knowledge. Participants who had more positive attitudes after training were significantly more likely to have had less prior supervision/mentoring. Reluctance to intervene was not found to significantly change after training.

Conclusions

We found evidence that attitudes, perceived capability and declarative knowledge changed positively from pre- to post-STARS training among mental health professionals. Underpinned by the minimum standardized SRA competencies, STARS training may be critical for informing evidence-based knowledge and skills in SRA and safety planning.
Bumetanide for Irritability in Children With Sensory Processing Problems Across Neurodevelopmental Disorders: A Pilot Randomized Controlled Trial
Authors: van Andel Dorinde M. , Sprengers Jan J. , Keijzer-Veen Mandy G. , Schulp Annelien J. A. , Lillien Marc R. , Scheepers Floortje E. , Bruining Hilgo
Published: February 2022

Background

Treatment development for neurodevelopmental disorders (NDDs) such as autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD) is impeded by heterogeneity in clinical manifestation and underlying etiologies. Symptom traits such as aberrant sensory reactivity are present across NDDs and might reflect common mechanistic pathways. Here, we test the effectiveness of repurposing a drug candidate, bumetanide, on irritable behavior in a cross-disorder neurodevelopmental cohort defined by the presence of sensory reactivity problems.

Methods

Participants, aged 5–15 years and IQ ≥ 55, with ASD, ADHD, and/or epilepsy and proven aberrant sensory reactivity according to deviant Sensory Profile scores were included. Participants were randomly allocated (1:1) to bumetanide (max 1 mg twice daily) or placebo tablets for 91 days followed by a 28-day wash-out period using permuted block design and minimization. Participants, parents, healthcare providers, and outcome assessors were blinded for treatment allocation. Primary outcome was the differences in ABC-irritability at day 91. Secondary outcomes were differences in SRS-2, RBS-R, SP-NL, BRIEF parent, BRIEF teacher at D91. Differences were analyzed in a modified intention-to-treat sample with linear mixed models and side effects in the intention-to-treat population.

Results

A total of 38 participants (10.1 [SD 3.1] years) were enrolled between June 2017 and June 2019 in the Netherlands. Nineteen children were allocated to bumetanide and nineteen to placebo. Five patients discontinued (n = 3 bumetanide). Bumetanide was superior to placebo on the ABC-irritability [mean difference (MD) −4.78, 95%CI: −8.43 to −1.13, p = 0.0125]. No effects were found on secondary endpoints. No wash-out effects were found. Side effects were as expected: hypokalemia (p = 0.046) and increased diuresis (p = 0.020).

Conclusion

Despite the results being underpowered, this study raises important recommendations for future cross-diagnostic trial designs.
Comparison of the Efficacy of Anatomic and Non-anatomic Hepatectomy for Hepatic Alveolar Echinococcosis: Clinical Experience of 240 Cases in a Single Center
Authors: A Jide , Zhang Jingni , Chai Jinping , Zhao Shunyun , Wang Hao , A Xiangren , Yang Jinyu
Published: February 2022

Background

Hepatic alveolar echinococcosis (AE) is a zoonotic parasitic disease. There are more than 16,000 new cases each year, approximately 60 million people are threatened, and the annual direct economic loss is RMB 3 billion. The prevalence of AE in some areas of the Qinghai–Tibet Plateau is as high as 6.0%. Radical resection, including anatomic and non-anatomic hepatectomy, for advanced AE can significantly prolong the survival time of patients. However, there is no literature compared the efficacy of anatomic and non-anatomic hepatectomy. Therefore, by comparing various clinical evaluation indices between anatomic and non-anatomic hepatectomy, this study explored the short-term and long-term efficacy of these two surgical methods for AE.

Methods

The clinical data of patients with AE who underwent radical hepatectomy at Qinghai Provincial People's Hospital from January 2015 to January 2021 were retrospectively analyzed. The patients were divided into two groups by surgical method, that were, non-anatomic hepatectomy group and anatomic hepatectomy group. We compared these two groups focusing on basic preoperative data, such as age, sex, lesion size, and liver function parameters; main intraoperative evaluation indices, such as operation time, intraoperative porta hepatis occlusion time, intraoperative blood loss, and blood transfusion; and postoperative recovery evaluation indicators, such as postoperative liver function, incidence of surgical complications, and AE recurrence.

Results

A total of 240 patients were enrolled in this study, including 123 in anatomic hepatectomy group and 117 in non-anatomic hepatectomy group. There were no significant differences (P > 0.05) between baseline characteristics. Anatomic hepatectomy group was advantageous than non-anatomic hepatectomy group regarding intraoperative blood loss (P < 0.001), blood transfusion (P < 0.001), and porta hepatis occlusion time (P < 0.001). There were statistically significant differences in postoperative liver function (aspartate aminotransferase: P < 0.001; alanine aminotransferase: P < 0.001), surgical complications (P < 0.001), and AE recurrence rate (P = 0.003). The median survival of patients in the anatomic hepatectomy group was 66 months, compared to 65 months in the non-anatomic hepatectomy group (χ2 = 4.662, P = 0.031).

Conclusions

Anatomic hepatectomy was not only safe for AE but also showed better short-term and long-term superiority than non-anatomic hepatectomy.
Universal Testing Policy for COVID-19 in Pregnancy: A Systematic Review
Authors: Hashim Nur Amirah Farhanah , Mahdy Zaleha Abdullah , Abdul Rahman Rahana , Kalok Aida Hani Mohd , Sutan Rosnah
Published: February 2022

Background

The coronavirus disease (COVID-19) has spread at an accelerated rate. WHO reported that in the general population, the majority are either asymptomatic or mildly infected. In view of the high risk of SARS-CoV-2 transmission from a pregnant woman to her newborn, healthcare workers and other patients, it is a raised concern whether universal testing should be implemented in this targeted population. The current guidelines have not recommended a universal testing policy. In certain European countries, however, the policy was implemented by some hospitals in regions with high prevalence of COVID-19 infection.

Aim(s)

To assess the justification for universal screening of pregnant women for COVID-19 prior to admission in labor through systematic review of antenatal prevalence of asymptomatic infection, hence risk of inadvertent spread of infection.

Materials and Methods

Three databases confined to PubMed, Ovid and Science Direct were used to search for articles from November 2019 onwards published in the English language. The search was conducted using the keywords “COVID-19” or “coronavirus” or “SARS-CoV-2” and “pregnancy” or “pregnant” or “obstetric” or “labor” and “universal” or “testing” or “prevalence”. The review was registered with PROSPERO.

Results

The search result retrieved 34 studies, with the majority consisting of retrospective cohort studies, while other studies such as prospective cohort study, research letters and a case series were also identified. A total of 19,958 pregnant women were universally tested until the date of report. Overall, the prevalence of universal testing among pregnant women presenting to labor and delivery units are higher in Western regions. From the total number of pregnant women 5.3% tested positive and among these, the majority (75.5%) did not manifest any symptoms at the time of testing.

Conclusion

In areas with high prevalence of COVID-19 infection, the implementation of a universal testing policy among pregnant women presenting to labor and admission units may be cost effective in helping to curb disease transmission.

Systematic Trial Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020184248, PROSPERO: CRD42020184248.
The Relationship Between Attentional Bias, Anxiety Sensitivity, and Depression and Anxiety Symptoms: Evidence From the COVID-19 Pandemic in China
Authors: Li Shiyi , Li Xiao
Published: February 2022

Background

The COVID-19 pandemic has led to observed increases in reported mental health issues, such as depression and anxiety symptoms. There is evidence attentional bias is associated with depression and anxiety, and it has been further suggested that anxiety sensitivity has a role in both the development and maintenance of depression and anxiety symptoms. Understanding these relationships may help inform preventative interventions for those at risk of mental health concerns. The present study explores the role of anxiety sensitivity, specifically physical and cognitive concerns, as a potential mediator of the relationship between attentional bias with depression and anxiety symptoms.

Method

Participants (n = 460) were recruited from the general population in China, and completed an online survey between February and March, 2020 which included the Attention to Positive and Negative Information Scale (APNI), Anxiety Sensitivity Index-3 (ASI-3) and Depression, Anxiety and Stress Scale (DASS-21). After exploring the correlations between the measures, mediation analysis was performed to explore the role of anxiety sensitivity (physical and cognitive subscales) in the relationship between attentional bias and depression and anxiety (as measured by the DASS-21).

Results

The results indicated that negative attention bias was significantly positively correlated with physical and cognitive concerns, physical and cognitive concerns were significantly positively correlated with depression and anxiety, and negative attention bias was significantly positively correlated with depression and anxiety (all ps < 0.001). Physical and cognitive anxiety sensitivity mediated the relationship between negative attention bias and both anxiety and depression symptoms.

Conclusion

Negative bias was associated with levels of anxiety and depression, and physical and cognitive anxiety sensitivity mediated associations between negative bias and anxiety and depression symptoms. The study provides theoretical support for intervention and guidance on individual mental health during the pandemic, and helps individuals increase their concern to negative emotions.
Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium
Authors: Takeuchi Hiroki , Nakamura Eriko , Yamaga Shunsuke , Amano Atsuo
Published: February 2022

Periodontal diseases initiate on epithelial surfaces of the subgingival compartment, while the gingival epithelium functions as an epithelial barrier against microbial infection and orchestrates immune responses. Porphyromonas gingivalis is a major pathogen of periodontal diseases and has an ability to penetrate the epithelial barrier. To assess the molecular basis of gingival epithelial barrier dysfunction associated with P. gingivalis, we newly developed a three-dimensional multilayered tissue model of gingival epithelium with gene manipulation. Using this novel approach, P. gingivalis gingipains including Arg- or Lys-specific cysteine proteases were found to specifically degrade junctional adhesion molecule 1 and coxsackievirus and adenovirus receptor in the tissue model, leading to increased permeability for lipopolysaccharide, peptidoglycan, and gingipains. This review summarizes the strategy used by P. gingivalis to disable the epithelial barrier by disrupting specific junctional adhesion molecules.
A Novel Building Performance Based Climate Zoning for Ethiopia
Authors: Zeleke Belay , Kumar Manoj , Rajasekar E.
Published: February 2022

Climate zoning plays a vital role in the development and implementation of building energy regulations. his paper presents a novel building performance-based approach for climate zoning. By using a high resolution spatial climate dataset, a climate severity mapping of Ethiopia is presented. Ethiopia represents 13 of the Köppen Geiger global climate zones. Real-time thermal performance measurement of representative residential buildings in three climatic locations is presented. Thermodynamic models of these buildings are developed and validated using energyplus software tool. Hourly building simulations of these buildings are performed for 1,490 locations (15 min spatial resolution) across Ethiopia. Cooling and heating discomfort hours, as well as energy performance index, are computed for each of these geolocations. Spatially interpolated building performance metrics and adaptive thermal comfort limits are presented. The relevance of Köppen Geiger's classification in the context of building performance is statistically tested. We observed that the existing climate zones do not considerably represent the building thermal performance and energy footprint. Effect of thermal severity on building performance is described. Further, climate zones are deduced based on the building performance variables using multivariate statistical clustering. In order to evaluate potential climate responsive strategies, bioclimatic zoning of Ethiopia is presented. The bioclimatic zoning is created using an improved Mahoney's method that incorporates solar radiation. This analysis resulted in 21 bioclimatic strategy zones. A comparative assessment of the new climate zoning with Mahoney's bioclimatic classification is presented. The proposed framework will be relevant for regulating building performance and energy conservation measures.
Filling the Protein Gap in Ghana: The Role of Soy
Authors: Atuna Richard Atinpoore , Amagloh Flora Christine , Denwar Nicholas Ninju , Asase Vincent Richard , Faisal Salifu , Baako Emmanuel , Koomson Gifty , Gulkirpik Ece , Toc Marco , Donnelly Annette , Amagloh Francis Kweku , Andrade Laborde Juan E.
Published: February 2022

The study assessed the nutrient value and desirability of eight improved soybean varieties, for use in soymilk, tofu and as an ingredient to enhance staple foods. The soymilk, tofu, and soybean residue (okara) yields were determined across all varieties. The okara was subsequently used in composite with cassava, as a recipe refinement of gari, a popular cassava-based ready-to-eat food. Multiple composite ratios were compared against a control of 100% cassava gari; 80% cassava: 20% okara, 70% cassava: 30% okara, and 50% cassava: 50% okara. The soymilk and tofu from the various varieties and okara enriched-gari were also evaluated for proximate and sensory qualities (n = 50) using standard protocols. No differences (p > 0.05) existed among soybean varieties in terms of soymilk (p = 0.55; 13.0–14.1 L), tofu (p = 0.05; 0.12–0.15 kg/L) or okara (p = 0.08; 3.17–3.97 kg) yields. The proximate parameters evaluated for soymilk did not vary significantly (p > 0.05) among varieties. However, for total solids (3.33–7.93°Brix; p < 0.01) there were significant differences. Generally, there was an increasing trend in the crude protein, moisture, crude fat and total ash contents for the okara-enriched gari as the okara inclusion increased from 20 to 50%. Thus, the crude protein content of the 50% okara-enriched gari, the formulation with the highest okara incorporation was almost 11-times higher than the 100% cassava gari. The swelling capacity of the okara-enriched gari ranged from 3.29–5.47 and for water holding capacity 439.7–482.1%. The okara-enriched gari was equally preferred by consumers, except for colour which consumers were mostly indifferent towards. The 50%-okara enriched gari composite was compared equally with 100% cassava gari control. The sensory data showed that the “Favour” soybean variety was desirable for soymilk production while Salintuya 1 was desirable for tofu production. Recipe refinements using the desired varieties and compositing okara with cassava may help fill the protein gap among the vulnerable group in Ghana by improving the protein quality of ready-to-eat foods such as gari.
Admission Homocysteine as a Potential Predictor for Delayed Cerebral Ischemia After Aneurysmal Subarachnoid Hemorrhage
Authors: Zhang Tiesong , Zhang Luyuan , Nie Kehui , Yang Jun , Lou Haiyan , Wang Jincheng , Huang Sicong , Gu Chenjie , Yan Min , Zhan Renya , Pan Jianwei
Published: February 2022

Background

Delayed cerebral ischemia (DCI) is a primary cause of poor prognosis after aneurysmal subarachnoid hemorrhage (aSAH) and needs close medical attention in clinical practice. Homocysteine (Hcy) has been implicated in cerebrovascular diseases. This study aimed to investigate whether serum Hcy could help to predict the occurrence of DCI in aSAH patients, and compare its diagnostic value with traditional methods.

Methods

We enrolled 241 aSAH patients in this study. Serum Hcy levels were collected from each patient. The baseline information was reviewed and analyzed. The binary logistic regression was used to explore the relation of serum Hcy levels with occurrence of DCI, and diagnostic performance of serum Hcy for predicting DCI was evaluated using a receiver operating characteristic (ROC) curve.

Results

The admission serum Hcy levels were found significantly higher in aSAH patients with DCI than those without (P < 0.001). The serum Hcy levels were positively correlated with the World Federation of Neurosurgical Societies (WFNS) scores, modified Fisher scores as well as Hunt and Hess scores at admission. Multivariate analysis revealed that occurrence of DCI was associated with serum Hcy levels (Odds Ratio [OR] = 1.257; 95% Confidence Interval [CI], 1.133–1.396, P < 0.001), modified Fisher scores (OR = 1.871; 95%CI, 1.111–3.150, P = 0.018) and Hunt and Hess scores (OR = 2.581; 95%CI, 1.222–5.452, P = 0.013) after adjusting for the significant variables in univariate analysis. Meanwhile, serum Hcy levels achieved good performance for DCI prediction (area under the curve [AUC], 0.781; 95%CI, 0.723–0.831, P < 0.001).

Conclusion

Serum homocysteine might have the potential to be a useful and cost-effective biomarker for predicting the occurrence of DCI in aSAH patients.
Using the Isolated Rat Placenta to Assess Fetoplacental Hemodynamics
Authors: Garner K. L. , Bowdridge E. C. , DeVallance E. , Griffith J. A. , Kelley E. E. , Nurkiewicz T. R.
Published: February 2022

Placental health is critical to fetal growth and maternal health during gestation. However, investigating placental flow in an ex-vivo isolated system where inflow is independently controlled has yet to be developed in the rat. Here, we describe a novel technique, isolated perfused placenta technique that allows for analysis of placental pressure outflow pressure, placental flow in rats at gestational day 20. Using this method, we successfully perfused placentas from dams and were able to observe increases in outflow pressure and flow as the inflow pressure to the placenta was increased in a step wise fashion. This method will help to advance the functional analysis of placental flow and therefore placental resistance and efficiency.
Spotted Fever Group Rickettsia Infecting Ticks (Acari: Ixodidae), Yak (Bos grunniens), and Tibetan Sheep (Ovis aries) in the Qinghai–Tibetan Plateau Area, China
Authors: He Yong-Cai , Li Ji-Xu , Sun Ya-Li , Kang Ming , He Hong-Xuan , Guo Yun-Hai , Ma Ping , Wei Yao-Ping , Li Rui-Shan , Chen Wang-Kai , Chen Zhi-Hong , Li Jing , Qi Tong-Sheng , Yang Jin-Fang , Zhang Qing-Xun , Wang Ye , Cai Jin-Shan , Zhao Quan-Bang , Hu Guang-Wei , Chen Ji-Yong , Li Ying
Published: February 2022

The Qinghai–Tibet Plateau Area (QTPA) has a complex natural ecosystem, causing a greatly increased risk of spreading various tick-borne diseases including rickettsial infections, which are regarded as one of the oldest known vector-borne zoonoses. However, the information of one of its pathogen, spotted fever group Rickettsia (SFG Rickettsia), is limited in tick vectors and animals in this area. Therefore, this study focused on the investigation of SFG Rickettsia in tick vectors, yaks (Bos grunniens), and Tibetan sheep (Ovis aries) in the QTPA. A total of 1,000 samples were collected from nine sampling sites, including 425 of yaks, 309 of Tibetan sheep, 266 of ticks. By morphological examination, PCR, and sequencing, we confirmed the species of all collected ticks. All tick samples, all yak and Tibetan sheep blood samples were detected based on SFG Rickettsia ompA and sca4 gene. The results showed that all tick samples were identified to be Haemaphysalis qinghaiensis, and the positive rates of SFG Rickettsia were 5.9% (25/425), 0.3% (1/309), and 54.1% (144/266) in yaks, Tibetan sheep, and ticks, respectively. All positive samples were sequenced, and BLASTn analysis of the ompA gene sequences of SFG Rickettsia showed that all positive samples from animals and ticks had 99.04–100% identity with yak and horse isolates from Qinghai Province, China. BLASTn analysis of the sca4 gene sequences of SFG Rickettsia showed that all positive samples had 97.60–98.72% identity with tick isolates from Ukraine. In addition, the phylogenetic analysis showed that all the SFG Rickettsia ompA and sca4 sequences obtained from this study belong to the same clade as Rickettsia raoultii isolated from livestock and ticks from China and other countries. Molecularly, this study detected and characterized SFG Rickettsia both in the tick vectors and animals, suggesting that the relationship between SFG Rickettsia, tick species and animal hosts should be explored to understand their interrelationships, which provide a theoretical basis for preventing control of this pathogen.
Infectious Bronchitis Virus Infection Increases Pathogenicity of H9N2 Avian Influenza Virus by Inducing Severe Inflammatory Response
Authors: Kong Lingchen , You Renrong , Zhang Dianchen , Yuan Qingli , Xiang Bin , Liang Jianpeng , Lin Qiuyan , Ding Chan , Liao Ming , Chen Libin , Ren Tao
Published: February 2022

Infectious bronchitis virus (IBV) and H9N2 avian influenza virus (AIV) are frequently identified in chickens with respiratory disease. However, the role and mechanism of IBV and H9N2 AIV co-infection remain largely unknown. Specific-pathogen-free (SPF) chickens were inoculated with IBV 2 days before H9N2 virus inoculation (IBV/H9N2); with IBV and H9N2 virus simultaneously (IBV+H9N2); with H9N2 virus 2 days before IBV inoculation (H9N2/IBV); or with either IBV or H9N2 virus alone. Severe respiratory signs, pathological damage, and higher morbidity and mortality were observed in the co-infection groups compared with the IBV and H9N2 groups. In general, a higher virus load and a more intense inflammatory response were observed in the three co-infection groups, especially in the IBV/H9N2 group. The same results were observed in the transcriptome analysis of the trachea of the SPF chickens. Therefore, IBV might play a major role in the development of respiratory disease in chickens, and secondary infection with H9N2 virus further enhances the pathogenicity by inducing a severe inflammatory response. These findings may provide a reference for the prevention and control of IBV and H9N2 AIV in the poultry industry and provide insight into the molecular mechanisms of IBV and H9N2 AIV co-infection in chickens.
Magnetic Resonance Imaging in 50 Captive Non-domestic Felids - Technique and Imaging Diagnoses
Authors: Hecht Silke , Cushing Andrew C. , Williams-Hagler Dottie A. , Craig Linden E. , Thomas William B. , Anderson Kimberly M. , Ramsay Edward C. , Conklin Gordon A.
Published: February 2022

Magnetic resonance imaging (MRI) is the recognized gold standard for diagnostic imaging of the central nervous system in human and veterinary patients. Information on the use of this modality and possible imaging abnormalities in captive non-domestic felids is currently limited to individual case reports or small case series. This retrospective study provides information on technique and imaging findings in a cohort of cases undergoing MRI at an academic Veterinary Medical Center. The University of Tennessee College of Veterinary Medicine MRI database was searched for non-domestic felids undergoing MRI of the brain or spine from 2008 to 2021. Medical record data were recorded, and MRI studies were reviewed. Fifty animals met the inclusion criteria. The most common brain diseases were Chiari-like malformation (n = 8) and inflammatory conditions (n = 8). Other abnormalities included pituitary lesions (n = 5), brain atrophy (n = 2), and one each of metabolic and traumatic conditions. Fourteen animals had a normal brain MRI study. The most common spinal abnormality was intervertebral disc disease (n = 7). Other disorders included vertebral dysplasia (n = 2), presumptive ischemic myelopathy (n = 1), subdural ossification causing spinal cord compression (n = 1), and multiple myeloma (n = 1). Spinal cord swelling of undetermined cause was suspected in two animals, and seven patients had a normal MRI study of the spine. MRI is a valuable tool in the diagnostic workup of non-domestic felids with presumptive neurologic disease.
Animal Health in Compost-Bedded Pack and Cubicle Dairy Barns in Six European Countries
Authors: Emanuelson Ulf , Brügemann Kerstin , Klopčič Marija , Leso Lorenzo , Ouweltjes Wijbrand , Zentner Andreas , Blanco-Penedo Isabel
Published: February 2022

The purpose of this study was to compare animal health in compost-bedded pack (CBP) and cubicle housing (CH) systems using data from dairy herd improvement associations. Thirty-two commercial dairy farms located in Austria, Germany, Italy, The Netherlands, Slovenia, and Sweden were included in the study. A matching design (pairing CBP and CH within country) according to herd selection criteria was used. We explored the following health indicators: somatic cell counts (SCC), high SCC, new high SCC, ketosis risk, prolonged calving intervals, dystocia, and stillbirth. Traits for culling and culling-related issues, such as length of life and length of productive life, were also included. We used multivariable (mixed) linear and logistic regression models to evaluate differences between the systems. Udder health, as measured by SCC, was inferior in CBP, although the geometric means were low in both systems. The incidence of stillbirths was higher in CBP, while prolonged calving intervals were fewer, indicating that there were fewer reproductive disorders. There were no differences in longevity between the systems, although CBP had lower proportions of first calvers. Overall, we conclude that there were few and minor differences in health and longevity between the CBP and CH systems in the European context.
Surface Gravity Wave Effect on Hurricane Energetics
Authors: Fan Yalin , Yu Zhitao
Published: February 2022

Theoretical researches have established that the energy dynamics of a mature tropical cyclone may be idealized to be very similar to a theoretical Carnot heat engine. Assuming the dissipative heating of the atmospheric boundary layer and the net production of mechanical energy in the cyclone dominate the energy budget of the storm, the potential maximum wind speed of the cyclone can be approximated as a function of the air–sea temperature difference (Ts − T0) and specific enthalpy (k0*−k) difference: |Vmax|2≈CkCDTs−T0T0(k0*−k). Although this theory gives a straighforward estimate of maximum tropical cyclone intensity, studies found that few real storms achieve this theoretical maximum estimated using climatological atmospheric conditions and sea surface temperatures. The discrepancies were attributed to a lack of knowledge of the values of the drag coefficient (CD) and surface exchange coefficient for enthalpy (Ck), and on insufficient upper ocean thermal measurements under hurricanes. Recent observational and numerical studies have unearthed another possible factor for these discrepancies by showing that the energy flux into surface gravity waves under extreme wind conditions can be an order of magnitude greater than formerly believed, and thus may play an important role in the energy budget of tropical cyclones. In this study, numerical experiments are performed to investigate the effect of surface gravity waves under a range of idealized tropical cyclone winds. The wave fields are simulated using the WAVEWATCH III model. Our results demonstrate that by considering the energy flux to surface gravity waves, the potential maximum wind speed can be reduced by up to 12% and this ratio varies with the storm size, intensity, and translation speed.
Latex Microsphere-Based Bicolor Immunochromatography for Qualitative Detection of Neutralizing Antibody against SARS-CoV-2
Authors: Liang Zhanwei , Peng Tao , Jiao Xueshima , Zhao Yang , Xie Jie , Jiang You , Meng Bo , Fang Xiang , Yu Xiaoping , Dai Xinhua
Published: February 2022

Neutralizing antibody (NAb) is a family of antibodies with special functions, which afford a degree of protection against infection and/or reduce the risk of clinically severe infection. Receptor binding domain (RBD) in the spike protein of SARS-CoV-2, a portion of the S1 subunit, can stimulate the immune system to produce NAb after infection and vaccination. The detection of NAb against SARS-CoV-2 is a simple and direct approach for evaluating a vaccine’s effectiveness. In this study, a direct, rapid, and point-of-care bicolor lateral flow immunoassay (LFIA) was developed for NAb against SARS-CoV-2 detection without sample pretreatment, and which was based on the principle of NAb-mediated blockage of the interaction between RBD and angiotensin-converting enzyme 2. In the bicolor LFIA, red and blue latex microspheres (LMs) were used to locate the test and control lines, leading to avoidance of erroneous interpretations of one-colored line results. Under the optimal conditions, NAb against SARS-CoV-2 detection carried out using the bicolor LFIA could be completed within 9 min, and the visible limit of detection was about 48 ng/mL. Thirteen serum samples were analyzed, and the results showed that the NAb levels in three positive serum samples were equal to, or higher than, 736 ng/mL. The LM-based bicolor LFIA allows one-step, rapid, convenient, inexpensive, and user-friendly determination of NAb against SARS-CoV-2 in serum.
White Matter Microstructural Alterations in Newly Diagnosed Parkinson’s Disease: A Whole-Brain Analysis Using dMRI
Authors: Kim Jun-Yeop , Shim Jae-Hyuk , Baek Hyeon-Man
Published: February 2022

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by cardinal motor symptoms and other non-motor symptoms. Studies have investigated various brain areas in PD by detecting white matter alterations using diffusion magnetic resonance imaging processing techniques, which can produce diffusion metrics such as fractional anisotropy and quantitative anisotropy. In this study, we compared the quantitative anisotropy of whole brain regions throughout the subcortical and cortical areas between newly diagnosed PD patients and healthy controls. Additionally, we evaluated the correlations between the quantitative anisotropy of each region and respective neuropsychological test scores to identify the areas most affected by each neuropsychological dysfunction in PD. We found significant quantitative anisotropy differences in several subcortical structures such as the basal ganglia, limbic system, and brain stem as well as in cortical structures such as the temporal lobe, occipital lobe, and insular lobe. Additionally, we found that quantitative anisotropy of some subcortical structures such as the basal ganglia, cerebellum, and brain stem showed the highest correlations with motor dysfunction, whereas cortical structures such as the temporal lobe and occipital lobe showed the highest correlations with olfactory dysfunction in PD. Our study also showed evidence regarding potential neural compensation by revealing higher diffusion metric values in early-stage PD than in healthy controls. We anticipate that our results will improve our understanding of PD’s pathophysiology.
Beyond Glioma: The Utility of Radiomic Analysis for Non-Glial Intracranial Tumors
Authors: Kalasauskas Darius , Kosterhon Michael , Keric Naureen , Korczynski Oliver , Kronfeld Andrea , Ringel Florian , Othman Ahmed , Brockmann Marc A.
Published: February 2022

The field of radiomics is rapidly expanding and gaining a valuable role in neuro-oncology. The possibilities related to the use of radiomic analysis, such as distinguishing types of malignancies, predicting tumor grade, determining the presence of particular molecular markers, consistency, therapy response, and prognosis, can considerably influence decision-making in medicine in the near future. Even though the main focus of radiomic analyses has been on glial CNS tumors, studies on other intracranial tumors have shown encouraging results. Therefore, as the main focus of this review, we performed an analysis of publications on PubMed and Web of Science databases, focusing on radiomics in CNS metastases, lymphoma, meningioma, medulloblastoma, and pituitary tumors.
Regulation of LncRNAs in Melanoma and Their Functional Roles in the Metastatic Process
Authors: Melixetian Marine , Pelicci Pier Giuseppe , Lanfrancone Luisa
Published: February 2022

Long non-coding RNAs (lncRNAs) are key regulators of numerous intracellular processes leading to tumorigenesis. They are frequently deregulated in cancer, functioning as oncogenes or tumor suppressors. As they act through multiple mechanisms, it is not surprising that they may exert dual functions in the same tumor. In melanoma, a highly invasive and metastatic tumor with the propensity to rapidly develop drug resistance, lncRNAs play different roles in: (i) guiding the phenotype switch and leading to metastasis formation; (ii) predicting the response of melanoma patients to immunotherapy; (iii) triggering adaptive responses to therapy and acquisition of drug resistance phenotypes. In this review we summarize the most recent findings on the lncRNAs involved in melanoma growth and spreading to distant sites, focusing on their role as biomarkers for disease diagnosis and patient prognosis, or targets for novel therapeutic approaches.
Early Bowel Lengthening Procedures: Bi-Institutional Experience and Review of the Literature
Authors: Negri Elisa , Coletta Riccardo , Forsythe Lynette , Gigola Francesca , Cianci Maria Chiara , Morabito Antonino
Published: February 2022

Early bowel lengthening procedure (EBLP) has been defined as any bowel lengthening procedure performed before six months of age. The purpose of this paper is to compare our experience with literature on this subject to identify common indications. A bi-institutional retrospective analysis was performed. Diagnosis, type of surgery, age at procedure and outcomes were analysed. Eleven EBLP were performed in Manchester and Florence from 2006 to 2021. The median age at surgery was 126 days (102–180), pre-operative median short bowel (SB) length was 28 cm (17–49) with a post-operative median increase of 81%. Furthermore, a PubMed/Embase search was undertaken regarding bowel lengthening procedures performed in the last 40 years. Sixty-one EBLP were identified. The median age was 60 days (1–90). Serial transverse enteroplasty (STEP) was the most frequent procedure used, with a median increased bowel length of 57%. This study confirms that no clear consensus on indication or timing to perform early SB lengthening is reported. According to the gathered data, EBLP should be considered only in cases of actual necessity and performed in a qualified intestinal failure centre.