bluuebunny/crossref_metadata_2025 huggingface dataset:
Competing Endogenous RNA Networks as Biomarkers in Neurodegenerative Diseases
Authors: Moreno-García Leticia , López-Royo Tresa , Calvo Ana Cristina , Toivonen Janne Markus , de la Torre Miriam , Moreno-Martínez Laura , Molina Nora , Aparicio Paula , Zaragoza Pilar , Manzano Raquel , Osta Rosario
Published: December 2020

Protein aggregation is classically considered the main cause of neuronal death in neurodegenerative diseases (NDDs). However, increasing evidence suggests that alteration of RNA metabolism is a key factor in the etiopathogenesis of these complex disorders. Non-coding RNAs are the major contributor to the human transcriptome and are particularly abundant in the central nervous system, where they have been proposed to be involved in the onset and development of NDDs. Interestingly, some ncRNAs (such as lncRNAs, circRNAs and pseudogenes) share a common functionality in their ability to regulate gene expression by modulating miRNAs in a phenomenon known as the competing endogenous RNA mechanism. Moreover, ncRNAs are found in body fluids where their presence and concentration could serve as potential non-invasive biomarkers of NDDs. In this review, we summarize the ceRNA networks described in Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis and spinocerebellar ataxia type 7, and discuss their potential as biomarkers of these NDDs. Although numerous studies have been carried out, further research is needed to validate these complex interactions between RNAs and the alterations in RNA editing that could provide specific ceRNET profiles for neurodegenerative disorders, paving the way to a better understanding of these diseases.
Identification and Characterization of a Novel Protein Disulfide Isomerase Gene (MgPDI2) from Meloidogyne graminicola
Authors: Tian Zhongling , Wang Zehua , Munawar Maria , Zheng Jingwu
Published: December 2020

Protein disulfide isomerase (PDI) is a multifunctional enzyme that catalyzes rate-limiting reactions such as disulfide bond formation, isomerization, and reduction. There is some evidence that indicates that PDI is also involved in host-pathogen interactions in plants. In this study, we show that the rice root-knot nematode, Meloidogyne graminicola, has evolved a secreted effector, MgPDI2, which is expressed in the subventral esophageal glands and up-regulated during the early parasitic stage of M. graminicola. Purified recombinant MgPDI2 functions as an insulin disulfide reductase and protects plasmid DNA from nicking. As an effector, MgPDI2 contributes to nematode parasitism. Silencing of MgPDI2 by RNA interference in the pre-parasitic second-stage juveniles (J2s) reduced M. graminicola multiplication and also increased M. graminicola mortality under H2O2 stress. In addition, an Agrobacterium-mediated transient expression assay found that MgPDI2 caused noticeable cell death in Nicotiana benthamiana. An intact C-terminal region containing the first catalytic domain (a) with an active motif (Cys-Gly-His-Cys, CGHC) and the two non-active domains (b and b′) is required for cell death induction in N. benthamiana. This research may provide a promising target for the development of new strategies to combat M. graminicola infections.
Current Genetic Survey and Potential Gene-Targeting Therapeutics for Neuromuscular Diseases
Authors: Chiu Wei , Hsun Ya-Hsin , Chang Kao-Jung , Yarmishyn Aliaksandr A. , Hsiao Yu-Jer , Chien Yueh , Chien Chian-Shiu , Ma Chun , Yang Yi-Ping , Tsai Ping-Hsing , Chiou Shih-Hwa , Lin Ting-Yi , Cheng Hao-Min
Published: December 2020

Neuromuscular diseases (NMDs) belong to a class of functional impairments that cause dysfunctions of the motor neuron-muscle functional axis components. Inherited monogenic neuromuscular disorders encompass both muscular dystrophies and motor neuron diseases. Understanding of their causative genetic defects and pathological genetic mechanisms has led to the unprecedented clinical translation of genetic therapies. Challenged by a broad range of gene defect types, researchers have developed different approaches to tackle mutations by hijacking the cellular gene expression machinery to minimize the mutational damage and produce the functional target proteins. Such manipulations may be directed to any point of the gene expression axis, such as classical gene augmentation, modulating premature termination codon ribosomal bypass, splicing modification of pre-mRNA, etc. With the soar of the CRISPR-based gene editing systems, researchers now gravitate toward genome surgery in tackling NMDs by directly correcting the mutational defects at the genome level and expanding the scope of targetable NMDs. In this article, we will review the current development of gene therapy and focus on NMDs that are available in published reports, including Duchenne Muscular Dystrophy (DMD), Becker muscular dystrophy (BMD), X-linked myotubular myopathy (XLMTM), Spinal Muscular Atrophy (SMA), and Limb-girdle muscular dystrophy Type 2C (LGMD2C).
Olive Production Threatened by a Resurgent Pest Liothrips oleae (Costa, 1857) (Thysanoptera: Phlaeothripidae) in Southern Italy
Authors: Vono Gregorio , Bonsignore Carmelo Peter , Gullo Gregorio , Marullo Rita
Published: December 2020

This study investigated a resurgence of Liothrips oleae Costa (Thysanoptera: Phlaeothripidae), an insect pest of olive crops, in a focal Southern Italian olive-producing area (Calabria Region). The young and adult olive thrips feed on the leaves and fruits of wild and cultivated olive trees, producing distortions, necrosis, and premature dropping of fruit. In our study, organic and integrated olive groves were compared for two years in order to establish the relationship between leaf and fruit damage among olive groves managed under different phytosanitary conditions. Sampling techniques were used in order to collect and count leaves and fruits (on plants and dropped premature drupes) presenting symptoms of thrips’ feeding activity. The impact of the thrips was significant in all orchards, and the estimated damage level on drupes and leaves was higher in organic olive management in each year. A morphological description of the adult females of the species is provided, and the first molecular characterization of the Calabrian olive thrips population was performed by using three different genetic regions (cytochrome c oxidase subunit I (COI), 28S ribosomal subunit (28S), and internal transcribed spacer 2 (ITS2)).
Experimental Study on Chloride Ion Diffusion in Concrete under Uniaxial and Biaxial Sustained Stress
Authors: Cheng Xiaokang , Peng Jianxin , Cai C.S. , Zhang Jianren
Published: December 2020

The existence of axial and lateral compressive stress affect the diffusion of chloride ions in concrete will lead to the performance degradation of concrete structure. This paper experimentally studied the chloride diffusivity properties of uniaxial and biaxial sustained compressive stress under one-dimensional chloride solution erosion. The influence of different sustained compressive stress states on chloride ion diffusivity is evaluated by testing chloride concentration in concrete. The experiment results show that the existence of sustained compressive stress does not always inhibit the diffusion of chloride ions in concrete, and the numerical value of sustained compressive stress level can affect the diffusion law of chloride ions in concrete. It is found that the chloride concentration decreases most when the lateral compressive stress level is close to 0.15 times the compressive strength of concrete. In addition, the sustained compressive stress has a significant effect on chloride ion diffusion of concrete with high water/cement ratio. Then, the chloride diffusion coefficient model under uniaxial and biaxial sustained compressive stress is established based on the apparent chloride diffusion coefficient. Finally, the results demonstrate that the chloride diffusion coefficient model is reasonable and feasible by comparing the experimental data in the opening literature with the calculated values from the developed model.
Bio-Based Packaging Materials Containing Substances Derived from Coffee and Tea Plants
Authors: Olejnik Olga , Masek Anna
Published: December 2020

The aim of the research was to obtain intelligent and eco-friendly packaging materials by incorporating innovative additives of plant origin. For this purpose, natural substances, including green tea extract (polyphenon 60) and caffeic acid, were added to two types of biodegradable thermoplastics (Ingeo™ Biopolymer PLA 4043D and Bioplast GS 2189). The main techniques used to assess the impact of phytocompounds on materials’ thermal properties were differential scanning calorimetry (DSC) and thermogravimetry (TGA), which confirmed the improved resistance to thermo-oxidation. Moreover, in order to assess the activity of applied antioxidants, the samples were aged using a UV aging chamber and a weathering device, then retested in terms of dynamic mechanical properties (DMA), colour changing, Vicat softening temperature, and chemical structure, as studied using FT-IR spectra analysis. The results revealed that different types of aging did not cause significant differences in thermo-mechanical properties and chemical structure of the samples with natural antioxidants but induced colour changing. The obtained results indicate that polylactide (PLA) and Bioplast GS 2189, the plasticizer free thermoplastic biomaterial containing polylactide and starch (referred to as sPLA in the present article), both with added caffeic acid and green tea extract, can be applied as smart and eco-friendly packaging materials. The composites reveal better thermo-oxidative stability with reference to pure materials and are able to change colour as a result of the oxidation process, especially after UV exposure, providing information about the degree of material degradation.
Effect of Shrinkage Reducing Admixture on Drying Shrinkage of Concrete with Different w/c Ratios
Authors: Kioumarsi Mahdi , Azarhomayun Fazel , Haji Mohammad , Shekarchi Mohammad
Published: December 2020

The reduction of the moisture content of concrete during the drying process reduces the concrete’s volume and causes it to shrink. In general, concrete shrinkage is a phenomenon that causes concrete volume to dwindle and can lead to durability problems. There are different types of this phenomenon, among them chemical shrinkage, autogenous shrinkage, drying shrinkage including free shrinkage and restrained shrinkage, and thermal contraction. Shrinkage-reducing admixtures are commercially available in different forms. The present study investigates the effect of liquid propylene glycol ether on mechanical properties and free shrinkage induced by drying at different water-cement (w/c) ratios. Furthermore, the effect of shrinkage-reducing admixtures on the properties of hardened concrete such as compressive and tensile strength, electrical resistivity, modulus of elasticity, free drying shrinkage, water absorption, and depth of water penetration was investigated. The results indicated that shrinkage reducing agents performed better in a low w/c ratio and resulted in up to 50% shrinkage reduction, which was due to the surface reduction of capillary pores. The prediction of free shrinkage due to drying was also performed using an artificial neural network.
Assessment of the Impact of Shot-Peening on the Fatigue Life of a Compressor Blade Subjected to Resonance Vibrations
Authors: Bednarz Arkadiusz , Misiolek Wojciech Z.
Published: December 2020

This publication presents an assessment of the influence of a surface treatment such as shot-peening on the fatigue life of a compressor blade exposed to resonant vibrations. As part of the work, a geometric model of the blade was developed, and a numerical modal and fatigue analysis were performed. The fatigue analysis was based on the Manson–Coffin–Basquin and Ramberg–Osgood models. Additionally, the location of the highest equivalent stresses was established. Based on the results of the strength analysis, two points were identified where a fatigue crack may potentially occur. As part of the work, the influence of different values of residual stresses on the results of the fatigue life was determined. The obtained results were compared to the literature values of fatigue life for this blade. A secondary objective of the study was to determine the size of the grains at various points of the blade, as well as the thickness of the layer plasticized as a result of peening. The relationship between the location of the highest values of the equivalent stresses and the thickness of the plasticized layer was determined. An explanation of the effect of shot-peening on the increase in the fatigue life of the blade was proposed.
Multi-Walled Carbon Nanotubes for Magnetic Solid-Phase Extraction of Six Heterocyclic Pesticides in Environmental Water Samples Followed by HPLC-DAD Determination
Authors: Ma Jiping , Hou Liwei , Wu Gege , Wang Liyan , Wang Xiaoyan , Chen Lingxin
Published: December 2020

Magnetic multi-walled carbon nanotubes were prepared as magnetic solid-phase extraction (MSPE) adsorbent for the enrichment of six heterocyclic pesticides in environmental water samples, including imidacloprid, triadimefon, fipronil, flusilazole, chlorfenapyr and fenpyroximate. Then six heterocyclic pesticides were separated and determined by high-performance liquid chromatography-diode-array detector (HPLC-DAD). Major factors influencing MSPE efficiency, including the dose of mag-multi-walled carbon nanotubes (mag-MWCNTs), extraction time, solution pH, salt concentration, type and volume of eluent and desorption time were investigated. Under the optimized conditions, the enrichment factor of the method reached to 250. The linearity was achieved within 0.05–10.0 μg/L for imidacloprid and chlorfenapyr, 0.10–10.0 μg/L for fipronil, flusilazole, triadimefon and fenpyroximate. Limits of detection were in the range of 0.01–0.03 μg/L. Good precision at three spiked levels were 1.1–11.2% (intra-day) and 1.7–11.0% (inter-day) with relative standard deviation of peak area, respectively. The developed method was utilized to analyze tap water, river water and reservoir water samples and recoveries at three spiked concentration levels ranged from 72.2% to 107.5%. The method was proved to be a convenient, rapid and practical method for sensitive determination of heterocyclic pesticides.
Microstructure–Property Relationship of Polyurethane Foams Modified with Baltic Sea Biomass: Microcomputed Tomography vs. Scanning Electron Microscopy
Authors: Kosmela Paulina , Suchorzewski Jan , Formela Krzysztof , Kazimierski Paweł , Haponiuk Józef Tadeusz , Piszczyk Łukasz
Published: December 2020

In this paper, novel rigid polyurethane foams modified with Baltic Sea biomass were compared with traditional petro-based polyurethane foam as reference sample. A special attention was focused on complex studies of microstructure, which was visualized and measured in 3D with high-resolution microcomputed tomography (microCT) and, as commonly applied for this purpose, scanning electron microscopy (SEM). The impact of pore volume, area, shape and orientation on appearance density and thermal insulation properties of polyurethane foams was determined. The results presented in the paper confirm that microcomputed tomography is a useful tool for relatively quick estimation of polyurethane foams’ microstructure, what is crucial especially in the case of thermal insulation materials.
Effect of Varying Steel Fiber Content on Strength and Permeability Characteristics of High Strength Concrete with Micro Silica
Authors: Ali Babar , Kurda Rawaz , Herki Bengin , Alyousef Rayed , Mustafa Rasheed , Mohammed Ahmed , Raza Ali , Ahmed Hawreen , Fayyaz Ul-Haq Muhammad
Published: December 2020

For the efficient and durable design of concrete, the role of fiber-reinforcements with mineral admixtures needs to be properly investigated considering various factors such as contents of fibers and potential supplementary cementitious material. Interactive effects of fibers and mineral admixtures are also needed to be appropriately studied. In this paper, properties of concrete were investigated with individual and combined incorporation of steel fiber (SF) and micro-silica (MS). SF was used at six different levels i.e., low fiber volume (0.05% and 0.1%), medium fiber volume (0.25% and 0.5%) and high fiber volume (1% and 2%). Each volume fraction of SF was investigated with 0%, 5% and 10% MS as by volume of binder. All concrete mixtures were assessed based on the results of important mechanical and permeability tests. The results revealed that varying fiber dosage showed mixed effects on the compressive (compressive strength and elastic modulus) and permeability (water absorption and chloride ion penetration) properties of concrete. Generally, low to medium volume fractions of fibers were useful in advancing the compressive strength and elastic modulus of concrete, whereas high fiber fractions showed detrimental effects on compressive strength and permeability resistance. The addition of MS with SF is not only beneficial to boost the strength properties, but it also improves the interaction between fibers and binder matrix. MS minimizes the negative effects of high fiber doses on the properties of concrete.
Direct Chill Casting and Extrusion of AA6111 Aluminum Alloy Formulated from Taint Tabor Scrap
Authors: Al-Helal Kawther , Patel Jayesh B. , Scamans Geoff M. , Fan Zhongyun
Published: December 2020

AA6111 aluminum automotive body-sheet alloy has been formulated from 100% Taint Tabor scrap aluminum. Direct chill casting with and without high shear melt conditioning (HSMC) was used to produce the AA6111 alloy billets. Both homogenized and non-homogenized billets were extruded into sheets. The optical micrographs of the melt conditioned direct chill (MC-DC) samples showed refined equiaxed grains in comparison to direct chill (DC) cast and direct chill grain refined (DC-GR) samples. Optical metallography showed extensive peripheral coarse grain (PCG) for the DC, DC-GR and MC-DC planks extruded from the homogenized standard AA6111 billets while planks extruded from modified AA6111 billets (with recrystallization inhibitors) showed thin PCG band. The co-addition of recrystallization inhibitors Mn, Zr, and Cr with elimination of the billet homogenization step had a favorable impact on the microstructure of the AA6111 alloy following the extrusion process where a fibrous grain structure was retained across the whole section of the planks. The mechanical properties of as-cast planks extruded from non-homogenized billets were similar to those extruded from homogenized billets. Eliminating the homogenization heat treatment step prior to extrusion has important ramifications in terms of processing cost reduction.
Determination of the Forming Limit for a ZIRLO™ Sheet with High Anisotropy
Authors: Kim Minsoo , Hong Seokmoo
Published: December 2020

In this study, the experimental two-dimensional forming limit diagram (FLD) data for a ZIRLO™ sheet, which is used in nuclear fuel rod support grids, were converted and presented as a triaxiality failure diagram (TFD). Most previous studies assumed ZIRLO™ to be isotropic when calculating the effective stress and strain. However, for highly anisotropic materials, the anisotropy should be considered for calculations of effective stress and strain; hence, in this study, they were calculated by introducing the normal anisotropy coefficient. To obtain this parameter of the ZIRLO™ specimens, tensile tests were performed on specimens with 0°, 45°, and 90° angles with respect to the rolling direction. It was observed that the average normal anisotropy coefficient measured during the tests was 4.94, which is very high. The von Mises isotropic and Hill 48 anisotropic yield criterion were applied to the FLD data that were experimentally determined using a limit dome height test and were converted into effective stress and effective strain. When the FLD is converted to TFD, the curve will increase in the top-right direction if the r-value is greater than 1, and this become more severe as the r-value increases. The TFD, which was converted considering the anisotropy, is almost the same to the TFD obtained using the digital image correlation method in the tensile tests of four specimens with different stress states. If anisotropy is not considered, then the formability is normally underestimated. However, a highly accurate TFD can be obtained with the method proposed in this study.
The Reducing Effects of Pyrogallol-Phloroglucinol-6,6-Bieckol on High-Fat Diet-Induced Pyroptosis in Endothelial and Vascular Smooth Muscle Cells of Mice Aortas
Authors: Oh Seyeon , Son Myeongjoo , Park Chul-Hyun , Jang Ji Tae , Son Kuk Hui , Byun Kyunghee
Published: December 2020

In hyperlipidemia, pyroptosis in endothelial cells (ECs) induces atherosclerosis via the toll-like receptor 4 (TLR4) pathway. We evaluated the effects of Ecklonia cava extract (ECE) and pyrogallol-phloroglucinol-6,6-bieckol (PPB) on pyroptosis of ECs and vascular smooth muscle cells (VSMCs), which leads to attenuation of these cells and dysfunction of the aorta in high-fat-diet (HFD)-fed mice and in palmitate-treated ECs and VSMCs. The expression of TLR4 and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which induce formation of NOD-LRR-and pyrin domain-containing protein 3 (NLRP3) inflammasomes, were increased by HFD and were decreased by ECE and PPB. The TLR4/NF-κB pathway was upregulated in palmitate-treated ECs and VSMCs and was decreased by ECE and PPB. The expressions of NLRP3/apoptosis-associated speck like protein containing a caspase recruitment domain, caspase-1, interleukin (IL)-1β, and IL-18 were increased by HFD and were decreased by ECE and PPB. Pyroptotic cells were increased by HFD and decreased by ECE and PPB. The expressions of the adhesion molecules, intercellular adhesion molecule and vascular cell adhesion molecule, and endothelin-1 were increased by HFD and were decreased by ECE and PPB. ECE and PPB decreased pyroptosis in the ECs and VSMCs, which was induced by HFD in the mouse aorta, and attenuated EC and VSMC dysfunction, an initiation factor of atherosclerosis.
Prevalence and Association of Transfusion Transmitted Infections with ABO and Rh Blood Groups among Blood Donors at the National Blood Bank, Amman, Jordan
Authors: Hroob Amir M. Al , Saghir Sultan A. M. , Almaiman Amer A. , Alsalahi Omar S. A. , Al-Wajeeh Abdullah S. , Al-Shargi Omar Y. A. , Al-Balagi Nader , Mahmoud Ayman M.
Published: December 2020

Background and objectives: Blood screening is considered a compulsory procedure in health care services to reduce the occurrence of transfusion transmitted infections (TTIs). This study estimated the distribution rates of ABO and Rh blood group systems, prevalence rates of TTIs among blood donors and their association with the ABO blood group and Rh system. Materials and Methods: A retrospective study was conducted at the national blood bank, Amman, Jordan for a period of 6 years (from January 2013 to December 2018). For TTIs analysis, about 5 mL blood sample was collected from each volunteer. A total of 365,029 persons (346,048 (94.8%) males and 18,981 (5.2%) females) donated their blood at the national blood bank, Amman, Jordan from January 2013 to December 2018. Results: The results revealed that O and A were the most prevalent blood groups (37.44% and 36.82%, respectively), followed by B (18.62%) and AB (7.12%). The distribution of Rh + ve and Rh − ve among blood donors showed that Rh + ve donors were more prevalent (88.73%) compared with Rh − ve (11.27%). HBsAg was the most prevalent viral infection (0.38%) followed by HCV (0.13%), syphilis (0.02%), HIV (0.006%) and the male donors were highly infected when compared with female donors. The association between ABO/Rh blood groups and TTIs infections was nonsignificant. Conclusions: In conclusion, low frequency rates of TTIs among blood donors were detected in the current study, but improvements are still continuously required. Low percentages of female donors need to be managed via conducting health cultural education programs.
Boron Nitride Nanotube (BNNT) Membranes for Energy and Environmental Applications
Authors: Yanar Numan , Yang Eunmok , Park Hosik , Son Moon , Choi Heechul
Published: December 2020

Owing to their extraordinary thermal, mechanical, optical, and electrical properties, boron nitride nanotubes (BNNTs) have been attracting considerable attention in various scientific fields, making it more promising as a nanomaterial compared to other nanotubes. Recent studies reported that BNNTs exhibit better properties than carbon nanotubes, which have been extensively investigated for most environment-energy applications. Irrespective of its chirality, BNNT is a constant wide-bandgap insulator, exhibiting thermal oxidation resistance, piezoelectric properties, high hydrogen adsorption, ultraviolet luminescence, cytocompatibility, and stability. These unique properties of BNNT render it an exceptional material for separation applications, e.g., membranes. Recent studies reported that water filtration, gas separation, sensing, and battery separator membranes can considerably benefit from these properties. That is, flux, rejection, anti-fouling, sensing, structural, thermal, electrical, and optical properties of membranes can be enhanced by the contribution of BNNTs. Thus far, a majority of studies have focused on molecular simulation. Hence, the requirement of an extensive review has emerged. In this perspective article, advanced properties of BNNTs are analyzed, followed by a discussion on the advantages of these properties for membrane science with an overview of the current literature. We hope to provide insights into BNNT materials and accelerate research for environment-energy applications.
Analytical Modeling of Residual Stress in Laser Powder Bed Fusion Considering Volume Conservation in Plastic Deformation
Authors: Mirkoohi Elham , Li Dongsheng , Garmestani Hamid , Liang Steven Y.
Published: December 2020

Residual stress (RS) is the most challenging problem in metal additive manufacturing (AM) since the build-up of high tensile RS may influence the fatigue life, corrosion resistance, crack initiation, and failure of the additively manufactured components. While tensile RS is inherent in all the AM processes, fast and accurate prediction of the stress state within the part is extremely valuable and results in optimization of the process parameters to achieve a desired RS and control of the build process. This paper proposes a physics-based analytical model to rapidly and accurately predict the RS within the additively manufactured part. In this model, a transient moving point heat source (HS) is utilized to determine the temperature field. Due to the high temperature gradient within the proximity of the melt pool area, the material experiences high thermal stress. Thermal stress is calculated by combining three sources of stresses known as stresses due to the body forces, normal tension, and hydrostatic stress in a homogeneous semi-infinite medium. The thermal stress determines the RS state within the part. Consequently, by taking the thermal stress history as an input, both the in-plane and out of plane RS distributions are found from the incremental plasticity and kinematic hardening behavior of the metal by considering volume conservation in plastic deformation in coupling with the equilibrium and compatibility conditions. In this modeling, material properties are temperature-sensitive since the steep temperature gradient varies the properties significantly. Moreover, the energy needed for the solid-state phase transition is reflected by modifying the specific heat employing the latent heat of fusion. Furthermore, the multi-layer and multi-scan aspects of metal AM are considered by including the temperature history from previous layers and scans. Results from the analytical RS model presented excellent agreement with XRD measurements employed to determine the RS in the Ti-6Al-4V specimens.
The Molecular and Pathophysiological Functions of Members of the LNX/PDZRN E3 Ubiquitin Ligase Family
Authors: Hong Jeongkwan , Won Minho , Ro Hyunju
Published: December 2020

The ligand of Numb protein-X (LNX) family, also known as the PDZRN family, is composed of four discrete RING-type E3 ubiquitin ligases (LNX1, LNX2, LNX3, and LNX4), and LNX5 which may not act as an E3 ubiquitin ligase owing to the lack of the RING domain. As the name implies, LNX1 and LNX2 were initially studied for exerting E3 ubiquitin ligase activity on their substrate Numb protein, whose stability was negatively regulated by LNX1 and LNX2 via the ubiquitin-proteasome pathway. LNX proteins may have versatile molecular, cellular, and developmental functions, considering the fact that besides these proteins, none of the E3 ubiquitin ligases have multiple PDZ (PSD95, DLGA, ZO-1) domains, which are regarded as important protein-interacting modules. Thus far, various proteins have been isolated as LNX-interacting proteins. Evidence from studies performed over the last two decades have suggested that members of the LNX family play various pathophysiological roles primarily by modulating the function of substrate proteins involved in several different intracellular or intercellular signaling cascades. As the binding partners of RING-type E3s, a large number of substrates of LNX proteins undergo degradation through ubiquitin-proteasome system (UPS) dependent or lysosomal pathways, potentially altering key signaling pathways. In this review, we highlight recent and relevant findings on the molecular and cellular functions of the members of the LNX family and discuss the role of the erroneous regulation of these proteins in disease progression.
Application 2D Descriptors and Artificial Neural Networks for Beta-Glucosidase Inhibitors Screening
Authors: Przybyłek Maciej
Published: December 2020

Beta-glucosidase inhibitors play important medical and biological roles. In this study, simple two-variable artificial neural network (ANN) classification models were developed for beta-glucosidase inhibitors screening. All bioassay data were obtained from the ChEMBL database. The classifiers were generated using 2D molecular descriptors and the data miner tool available in the STATISTICA package (STATISTICA Automated Neural Networks, SANN). In order to evaluate the models’ accuracy and select the best classifiers among automatically generated SANNs, the Matthews correlation coefficient (MCC) was used. The application of the combination of maxHBint3 and SpMax8_Bhs descriptors leads to the highest predicting abilities of SANNs, as evidenced by the averaged test set prediction results (MCC = 0.748) calculated for ten different dataset splits. Additionally, the models were analyzed employing receiver operating characteristics (ROC) and cumulative gain charts. The thirteen final classifiers obtained as a result of the model development procedure were applied for a natural compounds collection available in the BIOFACQUIM database. As a result of this beta-glucosidase inhibitors screening, eight compounds were univocally classified as active by all SANNs.
Homocoupling Reactions of Azoles and Their Applications in Coordination Chemistry
Authors: Mogensen Steffen B. , Taylor Mercedes K. , Lee Ji-Woong
Published: December 2020

Pyrazole, a member of the structural class of azoles, exhibits molecular properties of interest in pharmaceuticals and materials chemistry, owing to the two adjacent nitrogen atoms in the five-membered ring system. The weakly basic nitrogen atoms of deprotonated pyrazoles have been applied in coordination chemistry, particularly to access coordination polymers and metal-organic frameworks, and homocoupling reactions can in principle provide facile access to bipyrazole ligands. In this context, we summarize recent advances in homocoupling reactions of pyrazoles and other types of azoles (imidazoles, triazoles and tetrazoles) to highlight the utility of homocoupling reactions in synthesizing symmetric bi-heteroaryl systems compared with traditional synthesis. Metal-free reactions and transition-metal catalyzed homocoupling reactions are discussed with reaction mechanisms in detail.
Goethite Nanorods: Synthesis and Investigation of the Size Effect on Their Orientation within a Magnetic Field by SAXS
Authors: Hinrichs Stephan , Grossmann Larissa , Clasen Eike , Grotian genannt Klages Hannah , Skroblin Dieter , Gollwitzer Christian , Meyer Andreas , Hankiewicz Birgit
Published: December 2020

Goethite is a naturally anisotropic, antiferromagnetic iron oxide. Following its atomic structure, crystals grow into a fine needle shape that has interesting properties in a magnetic field. The needles align parallel to weak magnetic fields and perpendicular when subjected to high fields. We synthesized goethite nanorods with lengths between 200 nm and 650 nm in a two-step process. In a first step we synthesized precursor particles made of akaganeite (β-FeOOH) rods from iron(III)chloride. The precursors were then treated in a hydrothermal reactor under alkaline conditions with NaOH and polyvinylpyrrolidone (PVP) to form goethite needles. The aspect ratio was tunable between 8 and 15, based on the conditions during hydrothermal treatment. The orientation of these particles in a magnetic field was investigated by small angle X-ray scattering (SAXS). We observed that the field strength required to trigger a reorientation is dependent on the length and aspect ratio of the particles and could be shifted from 85 mT for the small particles to about 147 mT for the large particles. These particles could provide highly interesting magnetic properties to nanocomposites, that could then be used for sensing applications or membranes.
The lncRNA Toolkit: Databases and In Silico Tools for lncRNA Analysis
Authors: Pinkney Holly R. , Wright Brandon M. , Diermeier Sarah D.
Published: December 2020

Long non-coding RNAs (lncRNAs) are a rapidly expanding field of research, with many new transcripts identified each year. However, only a small subset of lncRNAs has been characterized functionally thus far. To aid investigating the mechanisms of action by which new lncRNAs act, bioinformatic tools and databases are invaluable. Here, we review a selection of computational tools and databases for the in silico analysis of lncRNAs, including tissue-specific expression, protein coding potential, subcellular localization, structural conformation, and interaction partners. The assembled lncRNA toolkit is aimed primarily at experimental researchers as a useful starting point to guide wet-lab experiments, mainly containing multi-functional, user-friendly interfaces. With more and more new lncRNA analysis tools available, it will be essential to provide continuous updates and maintain the availability of key software in the future.
Nutritional Interventions to Improve Asthma-Related Outcomes through Immunomodulation: A Systematic Review
Authors: van Brakel Lieve , Mensink Ronald P. , Wesseling Geertjan , Plat Jogchum
Published: December 2020

Asthma is a chronic inflammatory disease of the airways, characterized by T-helper (Th) 2 inflammation. Current lifestyle recommendations for asthma patients are to consume a diet high in fruits and vegetables and to maintain a healthy weight. This raises the question of whether other nutritional interventions may also improve asthma-related outcomes and whether these changes occur via immunomodulation. Therefore, we systematically reviewed studies that reported both asthma-related outcomes as well as immunological parameters and searched for relations between these two domains. A systematic search identified 808 studies, of which 28 studies met the inclusion criteria. These studies were divided over six nutritional clusters: herbs, herbal mixtures and extracts (N = 6); supplements (N = 4); weight loss (N = 3); vitamin D3 (N = 5); omega-3 long-chain polyunsaturated fatty acids (LCPUFAs) (N = 5); and whole-food approaches (N = 5). Fifteen studies reported improvements in either asthma-related outcomes or immunological parameters, of which eight studies reported simultaneous improvements in both domains. Two studies reported worsening in either asthma-related outcomes or immunological parameters, of which one study reported a worsening in both domains. Promising interventions used herbs, herbal mixtures or extracts, and omega-3 LCPUFAs, although limited interventions resulted in clinically relevant results. Future studies should focus on further optimizing the beneficial effects of nutritional interventions in asthma patients, e.g., by considering the phenotypes and endotypes of asthma.
Home Cooking Is Related to Potential Reduction in Cardiovascular Disease Risk among Adolescents: Results from the A-CHILD Study
Authors: Tani Yukako , Fujiwara Takeo , Isumi Aya , Doi Satomi
Published: December 2020

This study aimed to investigate the association between the frequency of home cooking and cardiovascular disease risk among Japanese adolescents. We used cross-sectional data on adolescents from the 2018 Adachi Child Health Impact of Living Difficulty study, which targeted junior high school students aged 13–14 years in Adachi, Tokyo, Japan. Frequency of home cooking by 553 caregivers was assessed via questionnaire and classified as high (almost daily), medium (4–5 days/week), or low (≤3 days/week). Cardiovascular disease risk factors included blood pressure, serum cholesterol (total, LDL, and HDL), hemoglobin A1c, and body mass index. Multiple linear regression analysis revealed that adolescents exposed to a low frequency of home cooking showed higher diastolic blood pressure (β = 3.59, 95% confidence interval [CI]: 0.42 to 6.75) and lower HDL cholesterol (β = −6.15, 95% CI: −11.2 to −1.07) than those exposed to a high frequency of home cooking, adjusting for adolescents’ sex, household income, and parental comorbidity. Future studies are needed to clarify the causal relationship and mechanisms through which home cooking influences adolescents’ cardiovascular health.
Chronic L-Name-Treatment Produces Hypertension by Different Mechanisms in Peripheral Tissues and Brain: Role of Central eNOS
Authors: Pechanova Olga , Vrankova Stanislava , Cebova Martina
Published: December 2020

The goal of our study was to analyze the time course of the effect of NG-nitro-L-arginine methyl ester (L-NAME) on nitric oxide synthase (NOS) isoforms and nuclear factor–κB (NF-κB) protein expression, total NOS activity, and blood pressure (BP) in rats. Adult 12-week-old male Wistar rats were subjected to treatment with L-NAME (40 mg/kg/day) for four and seven weeks. BP was increased after 4- and 7-week L-NAME treatments. NOS activity decreased after 4-week-L-NAME treatment; however, the 7-week treatment increased NOS activity in the aorta, heart, and kidney, while it markedly decreased NOS activity in the brainstem, cerebellum, and brain cortex. The 4-week-L-NAME treatment increased eNOS expression in the aorta, heart, and kidney and this increase was amplified after 7 weeks of treatment. In the brain regions, eNOS expression remained unchanged after 4-week L-NAME treatment and prolonged treatment led to a significant decrease of eNOS expression in these tissues. NF-κB expression increased in both peripheral and brain tissues after 4 weeks of treatment and prolongation of treatment decreased the expression in the aorta, heart, and kidney. In conclusion, decreased expression of eNOS in the brain regions after 7-week L-NAME treatment may be responsible for a remarkable decrease of NOS activity in these regions. Since the BP increase persisted after 7 weeks of L-NAME treatment, we hypothesize that central regulation of BP may contribute significantly to L-NAME-induced hypertension.
Nerve Response to Superelastic Shape Memory Polyurethane Aerogels
Authors: Sala Martina Rodriguez , Skalli Omar , Leventis Nicholas , Sabri Firouzeh
Published: December 2020

We have previously shown the suitability of aerogels as scaffolds for neuronal cells. Here, we report on the use of superelastic shape memory polyurethane aerogels (SSMPA). SSMPA have a distinctly different stiffness than previously reported aerogels. The soft and deformable nature of SSMPA allowed for radial compression of the aerogel induced by a custom designed apparatus. This radial compression changed the pore diameter and surface roughness (Sa) of SSMPA, while maintaining similar stiffness. Two varieties of SSMPA were used, Mix-14 and Mix-18, with distinctly different pore diameters and Sa. Radial compression led to a decreased pore diameter, which, in turn, decreased the Sa. The use of custom designed apparatus and two types of SSMPA allowed us to examine the influence of stiffness, pore size, and Sa on the extension of processes (neurites) by PC12 neuronal cells. PC12 cells plated on SSMPA with a higher degree of radial compression extended fewer neurites per cell when compared to other groups. However, the average length of the neurites was significantly longer when compared to the unrestricted group and to those extended by cells plated on SSMPA with less radial compression. These results demonstrate that SSMPA with 1.9 µm pore diameter, 1.17 µm Sa, and 203 kPa stiffness provides the optimum combination of physical parameters for nerve regeneration.
Hybrid Path Planning Combining Potential Field with Sigmoid Curve for Autonomous Driving
Authors: Lu Bing , He Hongwen , Yu Huilong , Wang Hong , Li Guofa , Shi Man , Cao Dongpu
Published: December 2020

The traditional potential field-based path planning is likely to generate unexpected path by strictly following the minimum potential field, especially in the driving scenarios with multiple obstacles closely distributed. A hybrid path planning is proposed to avoid the unsatisfying path generation and to improve the performance of autonomous driving by combining the potential field with the sigmoid curve. The repulsive and attractive potential fields are redesigned by considering the safety and the feasibility. Based on the objective of the shortest path generation, the optimized trajectory is obtained to improve the vehicle stability and driving safety by considering the constraints of collision avoidance and vehicle dynamics. The effectiveness is examined by simulations in multiobstacle dynamic and static scenarios. The simulation results indicate that the proposed method shows better performance on vehicle stability and ride comfortability than that of the traditional potential field-based method in all the examined scenarios during the autonomous driving.
Near-Field to Far-Field RCS Prediction on Arbitrary Scanning Surfaces Based on Spherical Wave Expansion
Authors: Kim Woobin , Im Hyeong-Rae , Noh Yeong-Hoon , Hong Ic-Pyo , Tae Hyun-Sung , Kim Jeong-Kyu , Yook Jong-Gwan
Published: December 2020

Near-field to far-field transformation (NFFFT) is a frequently-used method in antenna and radar cross section (RCS) measurements for various applications. For weapon systems, most measurements are captured in the near-field area in an anechoic chamber, considering the security requirements for the design process and high spatial costs of far-field measurements. As the theoretical RCS value is the power ratio of the scattered wave to the incident wave in the far-field region, a scattered wave measured in the near-field region needs to be converted into field values in the far-field region. Therefore, this paper proposes a near-field to far-field transformation algorithm based on spherical wave expansion for application in near-field RCS measurement systems. If the distance and angular coordinates of each measurement point are known, the spherical wave functions in an orthogonal relationship can be calculated. If each weight is assumed to be unknown, a system of linear equations as numerous as the number of samples measured in the near electric field can be generated. In this system of linear equations, each weight value can be calculated using the iterative least squares QR-factorization method. Based on this theory, the validity of the proposed NFFFT is verified for several scatterer types, frequencies and measurement distances.
Reliability of Trapezius Muscle Hardness Measurement: A Comparison between Portable Muscle Hardness Meter and Ultrasound Strain Elastography
Authors: Sawada Tomonori , Okawara Hiroki , Nakashima Daisuke , Iwabuchi Shuhei , Matsumoto Morio , Nakamura Masaya , Nagura Takeo
Published: December 2020

Prolonged computer work and smartphone use can cause stiffness of the neck and shoulder muscles, including the trapezius muscle. Hence, muscle hardness quantification is clinically beneficial. The present study aimed to examine the reliability of trapezius muscle hardness measurement using a portable muscle hardness meter and ultrasound strain elastography. Overall, 20 healthy young men participated in this study. Prior to measurement, the participant’s subjective symptoms, particularly shoulder muscle stiffness, were rated using an 11-point verbal scale. Furthermore, hardness of the right and left upper trapezius muscles was assessed. In the strain elastography assessment, muscle hardness was evaluated using strain ratio. Results showed that, in quantifying upper trapezius muscle hardness, both portable muscle hardness meter and strain elastography had an excellent intra-tester reliability (>0.9). However, the correlation coefficients between muscle hardness values assessed using a muscle hardness meter and those evaluated with strain elastography did not significantly differ, and the scores for subjective shoulder stiffness did not correspond to muscle hardness values. Therefore, the hardness of the trapezius muscle does not directly reflect the subjective shoulder stiffness. Future studies should thoroughly examine the location of the shoulder stiffness, and check whether it is accompanied by local pain or tenderness.
Assessment of the Electrostatic Separation Effectiveness of Plastic Waste Using a Vision System
Authors: Rybarczyk Dominik , Jędryczka Cezary , Regulski Roman , Sędziak Dariusz , Netter Krzysztof , Czarnecka-Komorowska Dorota , Barczewski Mateusz , Barański Mariusz
Published: December 2020

The work presented here describes the first results of an effective method of assessing the quality of electrostatic separation of mixtures of polymer materials. The motivation for the research was to find an effective method of mechanical separation of plastic materials and a quick assessment of the effectiveness of the method itself. The proposed method is based on the application of a dedicated vision system developed for needs of research on electrostatic separation. The effectiveness of the elaborated system has been demonstrated by evaluating the quality of the separation of mixtures of poly (methyl methacrylate) (PMMA) and polystyrene (PS). The obtained results show that the developed vision system can be successfully employed in the research on plastic separation, providing a fast and accurate method of assessing the purity and effectiveness of the separation process.