bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Nanotube Radius and the Volume Fraction on the Mechanical Properties of Carbon Nanotube-Reinforced Aluminum Metal Matrix Composites
Authors: Suk Myung Eun
Published: June 2021

By using the advantages of carbon nanotubes (CNTs), such as their excellent mechanical properties and low density, CNT-reinforced metal matrix composites (MMCs) are expected to overcome the limitations of conventional metal materials, i.e., their high density and low ductility. To understand the behavior of composite materials, it is necessary to observe the behavior at the molecular level and to understand the effect of various factors, such as the radius and content of CNTs. Therefore, in this study, the effect of the CNT radius and content on the mechanical properties of CNT-Al composites was observed using a series of molecular dynamics simulations, particularly focusing on MMCs with a high CNT content and large CNT diameter. The mechanical properties, such as the strength and stiffness, were increased with an increasing CNT radius. As the CNT content increased, the strength and stiffness increased; however, the fracture strain was not affected. The behavior of double-walled carbon nanotubes (DWNTs) and single-walled carbon nanotubes (SWNTs) was compared through the decomposition of the stress–strain curve and observations of the atomic stress field. The fracture strain increased significantly for SWNT-Al as the tensile force was applied in the axial direction of the armchair CNTs. In the case of DWNTs, an early failure was initiated at the inner CNTs. In addition, the change in the elastic modulus according to the CNT content was predicted using the modified rule of mixture. This study is expected to be useful for the design and development of high-performance MMCs reinforced by CNTs.
An Overview on Total Valorization of Litsea cubeba as a New Woody Oil Plant Resource toward a Zero-Waste Biorefinery
Authors: Qiu Yufei , Yu Yasi , Lan Ping , Wang Yong , Li Ying
Published: June 2021

With the increasing global demand for edible oils and the restriction of arable land minimum in China, woody oil plants have gradually become the optimal solution to cover the shortage of current edible oil supply and to further improve the self-sufficiency rate. However, due to the lack of knowledge and technique, problems like “how to make full use of these plant resources?” and “how to guide consumers with reasonable data?” limit the development of woody oilseed industry towards a sustainable circular economy. In this review, several emerging unique woody oil plants in China were introduced, among which Litsea cubeba as a new woody oil plant was highlighted as a reference case based on its current research progress. Unlike other woody oil plants, essential oil rather than oil from Litsea cubeba has always been the main product through the years due to its interesting biological activities. Most importantly, its major component, citral, could be the base for other synthesized perfume compounds with added value. Moreover, the sustainable biorefinery of large amounts of waste residual after Litsea cubeba essential oil processing is now technically feasible, which could inspire a total valorization pathway for other woody oil plants to make more competitive plant-based products with both economic, social, and ecological benefits.
Optimization of Ultrasound-Assisted Extraction of Phenolics from Sideritis raeseri Using Response Surface Methodology
Authors: Šavikin Katarina , Živković Jelena , Janković Teodora , Ćujić-Nikolić Nada , Zdunić Gordana , Menković Nebojša , Drinić Zorica
Published: June 2021

In this study we define the optimal conditions for ultrasound-assisted extraction of bioactive polyphenols from S. raeseri aerial parts using response surface methodology. The influence of ethanol concentration (10–90%), extraction temperature (20–80 °C), extraction time (10–60 min), and solid-to-solvent ratio (1:10–1:50) on total phenolic content as well as on content of individual flavonoids, and hypolaetin and isoscutellarein derivatives was studied. For the experimental design, a central composite design was chosen. In the obtained extracts, the following ranges of targeted compounds were detected: total phenol from 19.32 to 47.23 mg GAE/g dw, HYP from 1.05 to 11.46 mg/g dw, ISC 1 from 0.68 to 10.68 mg/g dw, and ISC 2 from 0.74 to 15.56 mg/g dw. The optimal extraction conditions were set as: ethanol concentration of 65%, extraction time of 50 min, extraction temperature of 63 °C, and solid-to-solvent ratio of 1:40. Contents of TP, HYP, ISC 1, and ISC 2 in optimal extracts were 47.11 mg GAE/g dw, 11.73 mg/g dw, 9.54 mg/g dw, and 15.40 mg/g dw, respectively. Experimentally set values were in good agreement with those predicted by the response surface methodology model, indicating suitability of the used model, as well as the success of response surface methodology in optimizing the conditions of the extraction.
DMSO Solubility Assessment for Fragment-Based Screening
Authors: Baybekov Shamkhal , Marcou Gilles , Ramos Pascal , Saurel Olivier , Galzi Jean-Luc , Varnek Alexandre
Published: June 2021

In this paper, we report comprehensive experimental and chemoinformatics analyses of the solubility of small organic molecules (“fragments”) in dimethyl sulfoxide (DMSO) in the context of their ability to be tested in screening experiments. Here, DMSO solubility of 939 fragments has been measured experimentally using an NMR technique. A Support Vector Classification model was built on the obtained data using the ISIDA fragment descriptors. The analysis revealed 34 outliers: experimental issues were retrospectively identified for 28 of them. The updated model performs well in 5-fold cross-validation (balanced accuracy = 0.78). The datasets are available on the Zenodo platform (DOI:10.5281/zenodo.4767511) and the model is available on the website of the Laboratory of Chemoinformatics.
PLGA Nanoparticles Decorated with Anti-HER2 Affibody for Targeted Delivery and Photoinduced Cell Death
Authors: Shipunova Victoria Olegovna , Sogomonyan Anna Samvelovna , Zelepukin Ivan Vladimirovich , Nikitin Maxim Petrovich , Deyev Sergey Mikhailovich
Published: June 2021

The effect of enhanced permeability and retention is often not sufficient for highly effective cancer therapy with nanoparticles, and the development of active targeted drug delivery systems based on nanoparticles is probably the main direction of modern cancer medicine. To meet the challenge, we developed polymer PLGA nanoparticles loaded with fluorescent photosensitive xanthene dye, Rose Bengal, and decorated with HER2-recognizing artificial scaffold protein, affibody ZHER2:342. The obtained 170 nm PLGA nanoparticles possess both fluorescent and photosensitive properties. Namely, under irradiation with the green light of 540 nm nanoparticles, they produced reactive oxygen species leading to cancer cell death. The chemical conjugation of PLGA with anti-HER2 affibody resulted in the selective binding of nanoparticles only to HER2-overexpressing cancer cells. HER2 is a receptor tyrosine kinase that belongs to the EGFR/ERbB family and is overexpressed in 30% of breast cancers, thus serving as a clinically relevant oncomarker. However, the standard targeting molecules such as full-size antibodies possess serious drawbacks, such as high immunogenicity and the need for mammalian cell production. We believe that the developed affibody-decorated targeted photosensitive PLGA nanoparticles will provide new solutions for ongoing problems in cancer diagnostics and treatment, as well in cancer theranostics.
Variability of the Chemical Composition of the Essential Oil from the Amazonian Ishpingo Species (Ocotea quixos)
Authors: Valarezo Eduardo , Vullien Antoine , Conde-Rojas Dayra
Published: June 2021

Ocotea quixos (Lam.) Kosterm. is an aromatic tree native to Ecuador, whose leaves are used to prepare aromatic beverages to which different health benefits are attributed. In this study, Ocotea quixos leaves were collected in the Amazon region in different environmental conditions and subjected to hydrodistillation to isolate the essential oil. The collection variables used were type of soil, amount of shade, and height; in addition, the presence of twig and leaf age and moisture were used as variables. Chemical composition was analyzed by means of gas chromatography equipped with a flame ionization detector and gas chromatography coupled to mass spectrometry. A wide variety of chemical compositions were detected in the samples. In total, forty-seven compounds were identified, which represented between 97.17% and 99.89% of the total composition. The constituents were mainly grouped into aliphatic sesquiterpene hydrocarbons (33.03–55.89%), other compounds (8.94–47.83%), and oxygenated monoterpenes (1.97–39.66%). The main constituents were found to be (E)-cinnamyl acetate (5.96–41.65%), (E)-methyl cinnamate (0.38–37.91%), and trans-caryophyllene (8.77–37.02%). The statistical analysis suggested the existence of two essential oil chemotypes and a direct correlation between environmental conditions and chemical composition of the essential oils.
Noncured Graphene Thermal Interface Materials for High-Power Electronics: Minimizing the Thermal Contact Resistance
Authors: Sudhindra Sriharsha , Kargar Fariborz , Balandin Alexander A.
Published: June 2021

We report on experimental investigation of thermal contact resistance, RC, of the noncuring graphene thermal interface materials with the surfaces characterized by different degree of roughness, Sq. It is found that the thermal contact resistance depends on the graphene loading, ξ, non-monotonically, achieving its minimum at the loading fraction of ξ ~15 wt%. Decreasing the surface roughness by Sq~1 μm results in approximately the factor of ×2 decrease in the thermal contact resistance for this graphene loading. The obtained dependences of the thermal conductivity, KTIM, thermal contact resistance, RC, and the total thermal resistance of the thermal interface material layer on ξ and Sq can be utilized for optimization of the loading fraction of graphene for specific materials and roughness of the connecting surfaces. Our results are important for the thermal management of high-power-density electronics implemented with diamond and other wide-band-gap semiconductors.
Enhancing Lithium and Sodium Storage Properties of TiO2(B) Nanobelts by Doping with Nickel and Zinc
Authors: Opra Denis P. , Gnedenkov Sergey V. , Sinebryukhov Sergey L. , Gerasimenko Andrey V. , Ziatdinov Albert M. , Sokolov Alexander A. , Podgorbunsky Anatoly B. , Ustinov Alexander Yu. , Kuryavyi Valery G. , Mayorov Vitaly Yu. , Tkachenko Ivan A. , Sergienko Valentin I.
Published: June 2021

Nickel- and zinc-doped TiO2(B) nanobelts were synthesized using a hydrothermal technique. It was found that the incorporation of 5 at.% Ni into bronze TiO2 expanded the unit cell by 4%. Furthermore, Ni dopant induced the 3d energy levels within TiO2(B) band structure and oxygen defects, narrowing the band gap from 3.28 eV (undoped) to 2.70 eV. Oppositely, Zn entered restrictedly into TiO2(B), but nonetheless, improves its electronic properties (Eg is narrowed to 3.21 eV). The conductivity of nickel- (2.24 × 10−8 S·cm−1) and zinc-containing (3.29 × 10−9 S·cm−1) TiO2(B) exceeds that of unmodified TiO2(B) (1.05 × 10−10 S·cm−1). When tested for electrochemical storage, nickel-doped mesoporous TiO2(B) nanobelts exhibited improved electrochemical performance. For lithium batteries, a reversible capacity of 173 mAh·g−1 was reached after 100 cycles at the current load of 50 mA·g−1, whereas, for unmodified and Zn-doped samples, around 140 and 151 mAh·g−1 was obtained. Moreover, Ni doping enhanced the rate capability of TiO2(B) nanobelts (104 mAh·g−1 at a current density of 1.8 A·g−1). In terms of sodium storage, nickel-doped TiO2(B) nanobelts exhibited improved cycling with a stabilized reversible capacity of 97 mAh·g−1 over 50 cycles at the current load of 35 mA·g−1.
MiRNA Expression in Neuroendocrine Neoplasms of Frequent Localizations
Authors: Korotaeva Alexandra , Mansorunov Danzan , Apanovich Natalya , Kuzevanova Anna , Karpukhin Alexander
Published: June 2021

Neuroendocrine neoplasms (NEN) are infrequent malignant tumors of a neuroendocrine nature that arise in various organs. They occur most frequently in the lungs, intestines, stomach and pancreas. Molecular diagnostics and prognosis of NEN development are highly relevant. The role of clinical biomarkers can be played by microRNAs (miRNAs). This work is devoted to the analysis of data on miRNA expression in NENs. For the first time, a search for specificity or a community of their functional characteristics in different types of NEN was carried out. Their properties as biomarkers were also analyzed. To date, more than 100 miRNAs have been characterized as differentially expressed and significant for the development of NEN tumors. Only about 10% of the studied miRNAs are expressed in several types of NEN; differential expression of the remaining 90% was found only in tumors of specific localizations. A significant number of miRNAs have been identified as potential biomarkers. However, only a few miRNAs have values that characterized their quality as markers. The analysis demonstrates the predominant specific expression of miRNA in each studied type of NEN. This indicates that miRNA’s functional features are predominantly influenced by the tissue in which they are formed.
Investigating Effects of Plasma Apolipoprotein E on Ischemic Heart Disease Using Mendelian Randomization Study
Authors: Li Meng-Yu , Kwok Man-Ki , Schooling Catherine Mary
Published: June 2021

Background: Observationally plasma apolipoprotein E (apoE) is positively associated with ischemic heart disease (IHD). A Mendelian randomization (MR) study suggesting apoE is unrelated to cardiovascular mortality did not consider specific isoforms. We used MR to obtain estimates of plasma apoE2, apoE3 and apoE4 on IHD, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol, triglycerides and apolipoprotein B (apoB). Methods: We obtained independent genetic instruments from proteome genome-wide association studies (GWAS) and applied them to large outcome GWAS. We used univariable MR to assess the role of each isoform and multivariable MR to assess direct effects. Results: In univariable MR, apoE4 was positively associated with IHD (odds ratio (OR) 1.05, 95% confidence interval (CI) 1.01 to 1.09), but apoE2 and apoE3 were less clearly associated. Using multivariable MR an association of apoE2 with IHD (OR 1.16, 95% CI 0.98 to 1.38) could not be excluded, and associations of apoE3 and apoE4 with IHD were not obvious. In univariable MR, apoE2 and apoE4 were positively associated with apoB, and a positive association of apoE2 with LDL cholesterol could not be excluded. Using multivariable MR apoE2 was positively associated with LDL cholesterol, and associations with apoB could not be excluded. After adjusting for apoB, no direct effects of apoE isoforms on IHD were evident. Conclusions: Plasma apoE2 and apoE4 may play a role in lipid modulation and IHD. Whether apoE could be a potential therapeutic target requires further clarification when larger genetic studies of apoE isoforms are available.
Immune Reconstitution Inflammatory Syndrome with Recurrent Paradoxical Cerebellar HIV-Associated Progressive Multifocal Leukoencephalopathy
Authors: Frattaroli Paola , Chueng Teresa A. , Abaribe Obinna , Ayoade Folusakin
Published: June 2021

Progressive multifocal leukoencephalopathy (PML), presenting as immune reconstitution inflammatory syndrome (IRIS), is a known complication of antiretroviral therapy (ART) in people living with HIV (PLWH). Typically preceded by ART initiation, IRIS may appear simultaneously/unmasked (PML-s-IRIS) or as a delayed/worsening/paradoxical (PML-d-IRIS) presentation of known PML disease. Primary cerebellar tropism continues to be a rare presentation, and paradoxical cerebellar involvement of PML-IRIS syndrome can be a challenge for both diagnosis and management. Steroids have been suggested as a possible therapy in severe cases but the duration of steroid therapy remain elusive. Our case is that of a 34-year-old man with newly diagnosed HIV simultaneously found to have cerebellar PML. His PML lesions however worsened after initiation of ART (PML-d-IRIS) with evidence of increased intracranial pressure. Despite initial favorable response to a short duration of steroids, he had multiple recurrence of his PML lesions after steroids were discontinued. The presence of predominant cerebellar lesions and the question of how long steroids should be provided to prevent or minimize PML recurrence is the highlight of our case. This report emphasizes the need for more controlled studies to assist clinicians in the optimal diagnosis and management of PML-IRIS in PLWH.
Research on the Coverage of Ultraviolet Communication Network in the Arbitrary Polygon Area
Authors: Cao Fang , Li Cheng , Xu Zhiyong
Published: June 2021

Given that the current ultraviolet (UV) networking model is established in a regular circular area, this article studies the coverage of a UV non-line-of-sight (NLOS) communication network creatively in the arbitrary polygon area. In this paper, the UV communication model and the basic concepts of network coverage are introduced first. Then the influence parameters of the UV node communication radius are studied, and the changes of the communication radius under different work patterns are analyzed. Finally, the coverage of the square target area is simulated under different communication parameters (transmitted power, data rate and node density). The results illustrate that the smaller the transceiver elevation angles are, the better the network coverage performance is. Additionally, we numerically compare the UV network models of polygonal and circular regions, which can be used as a reference for actual networking.
Color Digital Holography Based on Generalized Phase-Shifting Algorithm with Monitoring Phase-Shift
Authors: Jung Minwoo , Jeon Hosung , Lim Sungjin , Hahn Joonku
Published: June 2021

Color digital holography (DH) has been researched in various fields such as the holographic camera and holographic microscope because it acquires a realistic color object wave by measuring both amplitude and phase. Among the methods for color DH, the phase-shifting DH has an advantage of obtaining a signal wave of objects without the autocorrelation and conjugate noises. However, this method usually requires many interferograms to obtain signals for all wavelengths. In addition, the phase-shift algorithm is sensitive to the phase-shift error caused by the instability or hysteresis of the phase shifter. In this paper, we propose a new method of color phase-shifting digital holography with monitoring the phase-shift. The color interferograms are recorded by using a focal plane array (FPA) with a Bayer color filter. In order to obtain the color signal wave from the interferograms with unexpected phase-shift values, we devise a generalized phase-shifting DH algorithm. The proposed method enables the robust measurement in the interferograms. Experimentally, we demonstrate the proposed algorithm to reconstruct the object image with negligibly small conjugate noises.
A Broadband High-Diffraction-Efficiency Electro-Optic Bragg Deflector Based on Monolithic Dual-Grating Periodically-Poled Lithium Niobate
Authors: Chiang An-Chung , Lin Yuan-Yao , Lin Shou-Tai , Lin Yen-Yin
Published: June 2021

Electro-optic (EO) Bragg deflectors have been extensively used in a variety of applications. Recent developments show that bandwidths and deflection efficiencies, as well as angular bandwidths, would significantly limit the utilization of EO Bragg deflectors, especially for applications which need strong focusing, such as intra-cavity applications. In this paper, we introduce a broadband EO Bragg deflector based on periodically-poled lithium niobate with a monolithic dual-grating design. We analyzed the deflection properties of this device by using a modified coupled wave theory and showed that this device can be still efficient for a small beam radius under strong focusing, whereas a single-grating one becomes very inefficient. Using a 1064-nm laser beam with a 100-μm beam radius, we obtained a 74% deflection efficiency with a 190-V bias voltage with a 0.5-mm-thick and 7.5-mm-long dual-grating sample. The acceptance angle for the Bragg condition of this device is as large as a few tens of mrad. The potential bandwidth of this device exceeds 500 nm if the proper operation region is chosen.
Enhancing Energy Recovery in Form of Biogas, from Vegetable and Fruit Wholesale Markets By-Products and Wastes, with Pretreatments
Authors: Morales-Polo Carlos , Cledera-Castro María del Mar , Revuelta-Aramburu Marta , Hueso-Kortekaas Katia
Published: June 2021

Residues and by-products from vegetables and fruit wholesale markets are suitable for recovery in the form of energy through anaerobic digestion, allowing waste recovery and introducing them into the circular economy. This suitability is due to their composition, structural characteristics, and to the biogas generation process, which is stable and without inhibition. However, it has been observed that the proportion of methane and the level of degradation of the substrate is low. It is decided to study whether the effect of pretreatments on the substrate is beneficial. Freezing, ultrafreezing and lyophilization pretreatments are studied. A characterization of the substrates has been performed, the route of action of pretreatment determined, and the digestion process studied to calculate the generation of biogas, methane, hydrogen and the proportions among these. Also, a complete analysis of the process has been performed by processing the data with mathematical and statistical methods to obtain disintegration constants and levels of degradation. It has been observed that the three pretreatments have positive effects, when increasing the solubility of the substrate, increasing porosity, and improving the accessibility of microorganisms to the substrate. Generation of gases are greatly increased, reaching a methane enrichment of 59.751%. Freezing seems to be the best pretreatment, as it increases the biodegradation level, the speed of the process and the disintegration constant by 306%.
Transferability of ISSR, SCoT and SSR Markers for Chrysanthemum × Morifolium Ramat and Genetic Relationships Among Commercial Russian Cultivars
Authors: Samarina Lidia S. , Malyarovskaya Valentina I. , Reim Stefanie , Yakushina Lyudmila G. , Koninskaya Natalia G. , Klemeshova Kristina V. , Shkhalakhova Ruset M. , Matskiv Alexandra O. , Shurkina Ekaterina S. , Gabueva Tatiana Y. , Slepchenko Natalia A. , Ryndin Alexey V.
Published: June 2021

Characterization of genetic diversity in germplasm collections requires an efficient set of molecular markers. We assessed the efficiency of 36 new SCoT markers, 10 new ISSR markers, and 5 microsatellites for the characterization of genetic diversity in chrysanthemum core collection of 95 accessions (Russian and foreign cultivars). Seven new SCoT (SCoT12, 20, 21, 23, 29, 31, 34) and six new ISSR markers ((GA)8T, (CT)8G, (CTTCA)3, (GGAGA)3, (TC)8C, (CT)8TG) were efficient for the genetic diversity analysis in Chrysanthemum × morifolium collection. After STRUCTURE analysis, most Russian cultivars showed 20–50% of genetic admixtures of the foreign cultivars. Neighbor joining analysis based on the combination of SSR, ISSR, and SCoT data showed the best accordance with phenotype and origin compared to the separate analysis by each marker type. The position of the accessions within the phylogenetic tree corresponded with the origin and with some important traits, namely, plant height, stem and peduncle thickness, inflorescence type, composite flower and floret types, flower color, and disc color. In addition, several SCoT markers were suitable to separate the groups distinctly by the phenotypical traits such as plant height (SCoT29, SCoT34), thickness of the stem and peduncle (SCoT31, SCoT34), and leaf size and the floret type (SCoT31). These results provide new findings for the selection of markers associated with important traits in Chrysanthemum for trait-oriented breeding and germplasm characterization.
Ethylene Supplementation Combined with Split Application of Nitrogen and Sulfur Protects Salt-Inhibited Photosynthesis through Optimization of Proline Metabolism and Antioxidant System in Mustard (Brassica juncea L.)
Authors: Jahan Badar , Iqbal Noushina , Fatma Mehar , Sehar Zebus , Masood Asim , Sofo Adriano , D'Ippolito Ilaria , Khan Nafees A.
Published: June 2021

In the present study, the potential of ethylene as ethephon (an ethylene source) was investigated individually and in combination with split doses of nitrogen (N) and sulfur (S) soil treatments for removal of the damaging effects of salt stress (100 mM NaCl) in mustard (Brassica juncea L.). Plants were grown with 50 mg N plus 50 mg S kg−1 soil at sowing time and an equivalent dose at 20 days after sowing [N50 + S50]0d and 20d. Ethephon at 200 μL L‒1 was applied to combined split doses of N and S with or without NaCl. Plants subjected to NaCl showed a decrease in growth and photosynthetic characteristics as well as N and S assimilation, whereas proline metabolism and antioxidants increased. The application of ethephon to plants grown with split N and S doses significantly enhanced photosynthetic efficiency by increasing the assimilation of N and S, improving the concentration of proline and induction of the antioxidant system with or without NaCl. The regulation of ethylene and/or split forms of N and S application may be potential tools for not just overcoming salt stress effects in this species and in related Brassicaceae but also enhancing their photosynthesis and growth potential through increased nutrient assimilation.
Environmental and Management Effects on Demographic Processes in the U.S. Threatened Platanthera leucophaea (Nutt.) Lindl. (Orchidaceae)
Authors: Bell Timothy J. , Bowles Marlin L. , Zettler Lawrence W. , Pollack Catherine A. , Ibberson James E.
Published: June 2021

Populations of the U.S. threatened orchid, Platanthera leucophaea, are restricted to fragmented grassland and wetland habitats. We address the long-term (1998–2020) interactive effects of habitat (upland prairie vs. wetland), fire management (burned vs. unburned) and climatic variation, as well as pollination crossing effects, on population demography in 42 populations. Our analysis revealed the consistent interactive effects of habitat, dormant season burning, and climatic variation on flowering, reproduction, and survival. Burning increased flowering and population size under normal or greater than normal precipitation but may have a negative effect during drought years apparently if soil moisture stress reduces flowering and increases mortality. Trends in the number of flowering plants in populations also correspond to precipitation cycles. As with flowering and fecundity, survival is significantly affected by the interactive effects of habitat, fire, and climate. This study supports previous studies finding that P. leucophaea relies on a facultative outcrossing breeding system. Demographic modeling indicated that fire, normal precipitation, and outcrossing yielded greater population growth, and that greater fire frequency increased population persistence. It also revealed an ecologically driven demographic switch, with wetlands more dependent upon survivorship than fecundity, and uplands more dependent on fecundity than survivorship. Our results facilitate an understanding of environmental and management effects on the population demography of P. leucophaea in the prairie region of its distribution. Parallel studies are needed in the other habitats such as wetlands, especially in the eastern part of the range of the species, to provide a more complete picture.
Resistance Mechanism to Metsulfuron-Methyl in Polypogon fugax
Authors: Yu Xiaoyue , Wu Hanwen , Zhang Jianping , Yang Yongjie , Tang Wei , Lu Yongliang
Published: June 2021

Polypogon fugax is a common winter weed in China and other Asia countries. We have previously found a P. fugax biotype (R) resistant to acetyl co-enzyme A carboxylase (ACCase) herbicides also cannot be effectively controlled by some acetolactate synthase (ALS) herbicides. This study evaluated the level of resistance to four ALS herbicides (metsulfuron-methyl, chlorsulfuron, monosulfuron, pyribambenz isopropyl) in the R biotype and the associated resistance mechanism. The R biotype exhibited moderate level of resistance to metsulfuron-methyl (6.0-fold) compared with the sensitive biotype (S). Sequence analysis of ALS gene revealed that two ALS genes existed in P. fugax. However, no substitution associated with ALS resistance mechanism were found in ALS genes between the S and R biotypes. The activity of ALS enzyme isolated from the R biotype was inherently higher and less sensitive to metsulfuron-methyl than the S biotype. Glutathione S-transferases (GST) activity was also less sensitive to metsulfuron-methyl in the R than as the S biotypes. Malathion, a cytochrome P450 (CYP) monooxygenase inhibitor, had much greater synergistic effect with metsulfuron-methyl on the R than as the S plants, reducing the ED50 value (herbicide dose to inhibit growth by 50%) of metsulfuron-methyl by 23- and 6-fold, respectively, suggesting that CYP mediated enhanced metabolism might contribute to the resistance to ALS herbicides. These results suggest that metsulfuron-methyl resistance in the R biotype was associated with the up-regulated ALS enzymatic activity and the GST and CYP-mediated enhanced herbicide metabolism.
Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole
Authors: Chmur Magdalena , Bajguz Andrzej
Published: June 2021

Brassinolide (BL) represents brassinosteroids (BRs)—a group of phytohormones that are essential for plant growth and development. Brassinazole (Brz) is as a synthetic inhibitor of BRs’ biosynthesis. In the present study, the responses of Wolffia arrhiza to the treatment with BL, Brz, and the combination of BL with Brz were analyzed. The analysis of BRs and Brz was performed using LC-MS/MS. The photosynthetic pigments (chlorophylls, carotenes, and xanthophylls) levels were determined using HPLC, but protein and monosaccharides level using spectrophotometric methods. The obtained results indicated that BL and Brz influence W. arrhiza cultures in a concentration-dependent manner. The most stimulatory effects on the growth, level of BRs (BL, 24-epibrassinolide, 28-homobrassinolide, 28-norbrassinolide, catasterone, castasterone, 24-epicastasterone, typhasterol, and 6-deoxytyphasterol), and the content of pigments, protein, and monosaccharides, were observed in plants treated with 0.1 µM BL. Whereas the application of 1 µM and 10 µM Brz caused a significant decrease in duckweed weight and level of targeted compounds. Application of BL caused the mitigation of the Brz inhibitory effect and enhanced the BR level in duckweed treated with Brz. The level of BRs was reported for the first time in duckweed treated with BL and/or Brz.
Gene Expression and Isoform Identification of PacBio Full-Length cDNA Sequences for Berberine Biosynthesis in Berberis koreana
Authors: Roy Neha Samir , Choi Ik-Young , Um Taeyoung , Jeon Mi Jin , Kim Bo-Yun , Kim Young-Dong , Yu Ju-Kyung , Kim Soonok , Kim Nam-Soo
Published: June 2021

Berberis koreana is a medicinal plant containing berberine, which is a bioactive compound of the benzylisoquinoline alkaloid (BIA) class. BIA is widely used in the food and drug industry for its health benefits. To investigate the berberine biosynthesis pathway, gene expression analysis was performed in leaves, flowers, and fruits at different stages of growth. This was followed by full-length cDNA sequencing analysis using the PacBio sequencer platform to determine the number of isoforms of those expressed genes. We identified 23,246 full-length unigenes, among which 8479 had more than one isoform. The number of isoforms ranged between two to thirty-one among all genes. Complete isoform analysis was carried out on the unigenes encoding BIA synthesis. Thirteen of the sixteen genes encoding enzymes for berberine synthesis were present in more than one copy. This demonstrates that gene duplication and translation into isoforms may contribute to the functional specificity of the duplicated genes and isoforms in plant alkaloid synthesis. Our study also demonstrated the streamlining of berberine biosynthesis via the absence of genes for enzymes of other BIAs, but the presence of all the genes for berberine biosynthesize in B. koreana. In addition to genes encoding enzymes for the berberine biosynthesis pathway, the genes encoding enzymes for other BIAs were not present in our dataset except for those encoding corytuberine synthase (CTS) and berbamunine synthase (BS). Therefore, this explains how B. koreana produces berberine by blocking the pathways leading to other BIAs, effectively only allowing the pathway to lead to berberine synthesis.
Electrical Stimulation Enhances Plant Defense Response in Grapevine through Salicylic Acid-Dependent Defense Pathway
Authors: Mori Daisuke , Moriyama Ayane , Kanamaru Hiroshi , Aoki Yoshinao , Masumura Yoshiyuki , Suzuki Shunji
Published: June 2021

Concern over environmental pollution generated by chemical fungicides has led to the introduction of alternative pest management strategies to chemical fungicide application. One of those strategies is the induction of plant defense response by an abiotic elicitor. In the present study, field-grown grapevines were subjected to electrical stimulation using a solar panel from two weeks before flowering to harvest in the 2016 and 2020 growing seasons. In both years, electrical stimulation decreased the incidence of gray mold and/or ripe rot on bunches and downy mildew on leaves of the field-grown grapevine. Transcription of a gene encoding β-1,3-glucanase but not class IV chitinase in leaves of potted grapevine seedlings was upregulated 20 days after electrical stimulation, suggesting that electrical stimulation acts as an abiotic elicitor of plant defense response to fungal diseases. The gene expression of PR1 but not PDF1.2 was upregulated in Arabidopsis plants subjected to electrical stimulation. On the other hand, PR1 gene expression was not induced in salicylic acid (SA)-insensitive Arabidopsis mutant npr1-5 subjected to electrical stimulation. Taken together, electrical stimulation is responsible for plant defense response through the SA-dependent defense pathway. These findings would help us develop a novel and innovative practical technique that uses electrical stimulation in integrated pest management.
Improvements of Micro-CHP SOFC System Operation by Efficient Dynamic Simulation Methods
Authors: Nousch Laura , Hartmann Mathias , Michaelis Alexander
Published: June 2021

Solid Oxide Fuel Cell (SOFC) technology is of high interest for stationary decentralized generation of electricity and heat in combined heat and power systems (CHP) for the residential sector. Application scenarios for SOFC systems in an electricity-regulated mode play an important role, especially in places where an electrical grid connection is not available or rather unstable. The advantages of SOFC systems are the high fuel flexibility and the high efficiencies also under partial load operation compared to other decentralized power generation technologies. Due to the long, energy-consuming system heat-up and the limited partial load capability, SOFC systems do not reach the performance of conventional power generation technologies. Furthermore, stack thermal cycling is associated with power degradation and should be minimized. In this paper, the improvement of these drawbacks are investigated for hotbox-based SOFC systems in the 1 kWel-class for residential applications. Since experimental investigations of the high-temperature systems are limited, modeling tools are established, enabling the visualization of internal system characteristics and providing the opportunity to simulate system operation in critical regions. To achieve this, a methodology for dynamic SOFC system modeling in a process engineering manner is developed based on the modeling language Modelica. A suitable approach is particularly important for modeling and simulation of the strong thermal interaction between the hot system components within the hotbox. The parametrized and validated models are used for the investigation of different dynamic effects, such as the system heat-up and the operation in low partial load points. A second reduced thermal system model aims for annual simulations of the SOFC system together with a battery to investigate the number of thermal cycles and the advantage of a hot standby operation. As a result, it is found that an adequate control of the power input at the start-up device and the cathode air flow has a high improvement potential to increase the stack heating rate and accelerate the heat-up in an energy-saving way. The hotbox-internal thermal management is identified as a crucial issue to reach low partial load points. To avoid the risk of stack cooling, lower heat losses and/or additional heat sources are of importance. Furthermore, the robustness of the tail gas oxidizer is found to be crucial for a higher load flexibility during partial load and the end of life stack operation. The annual simulation results indicate that operating the battery hybrid system with a hot standby mode requires much lower battery capacity for a high grid independence and a complete avoidance of system shutdown and associated power degradation.
A Time-Series Data Generation Method to Predict Remaining Useful Life
Authors: Ahn Gilseung , Yun Hyungseok , Hur Sun , Lim Siyeong
Published: June 2021

Accurate predictions of remaining useful life (RUL) of equipment using machine learning (ML) or deep learning (DL) models that collect data until the equipment fails are crucial for maintenance scheduling. Because the data are unavailable until the equipment fails, collecting sufficient data to train a model without overfitting can be challenging. Here, we propose a method of generating time-series data for RUL models to resolve the problems posed by insufficient data. The proposed method converts every training time series into a sequence of alphabetical strings by symbolic aggregate approximation and identifies occurrence patterns in the converted sequences. The method then generates a new sequence and inversely transforms it to a new time series. Experiments with various RUL prediction datasets and ML/DL models verified that the proposed data-generation model can help avoid overfitting in RUL prediction model.
Environmental Performance of Alternative Green Polyol Synthesis Routes: A Proposal for Improvement
Authors: Carvalho Kaccnny , Alves Rita M. B. , Kulay Luiz
Published: June 2021

This study verified the environmental effectiveness of potentially less aggressive routes for the synthesis of poly(propylene/ethylene oxide) glycol (PPG). The analysis was developed in two stages. Firstly, the environmental performance of the conventional PPG processing route was compared to alternative variants—vegetal PPG and CO2-based PPG—applying the life cycle assessment technique to measure the primary energy demand, global warming potential, acidification, photochemical oxidation, and freshwater ecotoxicity impact categories. The synthesis of vegetable polyols from bio-based assets, such as vegetable oils, and the application of CO2 conversion routes as an alternative to technologies supported by petroleum and natural gas were studied. The use of CO2 recovered through carbon capture and usage practices resulted in environmental gains for PPG production. The processing routes within vegetal assets were not an environmentally attractive option as the performance was worse than the conventional arrangement by 144% for the global warming impact category, an increase related to the deforestation carried out to expand soybean cultivation in Brazil. Secondly, improvement scenarios to mitigate the environmental impacts of alternative routes were performed. The hypothesis of using cleaner inputs to obtain a more ecofriendly route was tested. The analysis concluded that the use of high-purity CO2 brings fewer benefits compared to other capturing sources that need a purification process before feeding the PPG synthesis.
Simulation and Optimisation of Integrated Anaerobic-Aerobic Bioreactor (IAAB) for the Treatment of Palm Oil Mill Effluent
Authors: Chong Jun Wei Roy , Chan Yi Jing , Chong Siewhui , Ho Yeek Chia , Mohamad Mardawani , Tan Wen Nee , Cheng Chin Kui , Lim Jun Wei
Published: June 2021

This study highlights an innovative piece of hybrid technology, whereby the combination of anaerobic and aerobic processes into a single reactor, namely, the integrated anaerobic–aerobic bioreactor (IAAB) can surpass the limits of conventional methods treating palm oil mill effluent (POME). Optimisation of IAAB using SuperPro Designer V9 simulator for maximum biogas yield while addressing its economic and environmental trade-offs was conducted for the first time. Parameters such as hydraulic retention time (HRT) and organic loading rate (OLR) were optimised in the anaerobic compartment from 10 days and 6.2 g COD/L day to 9 days and 6.9 g COD/L day, respectively. Furthermore, sludge recycle ratio was optimised from 20% to 50% in the aerobic compartment. The optimisation was successful where the biogas yield increased from 0.24 to 0.29 L CH4/g CODremoved with excellent Chemical Oxygen Demand (COD), and Biological Oxygen Demand (BOD) removal efficiencies up to 99% with 5.8% lower net expenditure. This simulation results were comparable against the pre-commercialized IAAB with 11.4% increase in methane yield after optimisation. Economic analysis had proven the optimised process to be feasible, resulting in return on investment (ROI), payback time, and internal rate of return (IRR) of 24.5%, 4.1 years, and 17.9%, respectively.
Did You Ask for Citations? An Insight into Preprint Citations en route to Open Science
Authors: Pagliaro Mario
Published: June 2021

This study investigates citation patterns between 2017 and 2020 for preprints published in three preprint servers, one specializing in biology (bioRxiv), one in chemistry (ChemRxiv), and another hosting preprints in all disciplines (Research Square). Showing evidence that preprints are now regularly cited in peer reviewed journal articles, books, and conference papers, the outcomes of this investigation further substantiate the value of open science also in relation to citation-based metrics on which the evaluation of scholarship continues to rely on. This analysis will be useful to inform new research-based education in today’s scholarly communication.
Faith, Religion, and Spirituality: A Phenomenological and Hermeneutic Contribution to Parsing the Distinctions
Authors: Gschwandtner Christina M.
Published: June 2021

Religion and spirituality are contested terms in the fields of Religious Studies, Theology, Sociology or Anthropology of Religion, and other areas, and the notion of faith has often been abandoned altogether. The present article attempts to make a distinctly philosophical contribution to this debate by employing phenomenological parameters, as they are articulated in the work of Martin Heidegger, for proposing distinctions between faith, religion, and spirituality. It then goes on to “fill” these structural distinctions in more detail with hermeneutic content by drawing on Paul Ricœur’s work on faith and religion, as well as Johann Michel’s analysis of Ricœur’s account of the self as a “spirituality”. The article thus employs Heidegger’s phenomenological categories and Ricœur’s hermeneutic project in order to think through the possibility of making phenomenological distinctions between personal confession of faith, religious adhesion to a tradition via myth and ritual, and a broader spirituality as a fundamental dimension of the human being.
Heidegger as Levinas’s Guide to Judaism beyond Philosophy
Authors: Lapidot Elad
Published: June 2021

This essay reflects on the way that Emmanuel Levinas stages the difference between Judaism and Philosophy, namely how he approaches Jewish thought as a concrete other of philosophy. The claim is that this mise en scène underlies Levinas’s oeuvre not only as a discourse about the Other, but as a real scene of an actual encounter with otherness, namely the encounter of philosophy with the epistemic otherness of Judaism. It is in the turn to Jewish thought beyond Philosophy that the essay identifies Heidegger’s strongest influence on Levinas. The essay’s reflection is performed through a reading of Levinas’s first major philosophical work of 1961, Totality and Infinity. The encounter between Philosophy and Judaism is explored in this context both as an epistemic and as a political event.
Measurements and Trends in Technological Eco-Innovation: Evidence from Environment-Related Patents
Authors: Urbaniec Maria , Tomala Justyna , Martinez Sergio
Published: June 2021

The concept of eco-innovation addresses a reduction in negative environmental impacts and the more efficient use of resources. As an integral part of eco-innovation, green technologies are receiving increasing attention due to growing environmental concerns. Patent data are one of the measures of the output of technological eco-innovation. However, understanding the patenting of eco-innovation comes with challenges. The aim of this study is to measure the output of eco-innovation and to analyse the trends in green technologies based on environment-related patents in the world’s leading countries from 2000 to 2017. For this research, a range of data collection techniques based on patent data from leading countries such as China, Korea, Japan, United States and Germany were employed. The study provides a comprehensive overview of changes and trends in the development of environmental technologies using different domains. In particular, significant progress has been made in the areas of environmental technologies and climate change mitigation technologies related to energy generation, transmission or distribution. These technologies are closely linked to international environmental policies such as climate change mitigation and green industry transformation. The study also contributes to the literature on measuring the output of eco-innovation.
Random Beam-Based Non-Orthogonal Multiple Access for Low Latency K-User MISO Broadcast Channels
Authors: Lee Jung Hoon , Kim Yunjoo , Ryu Jong Yeol
Published: June 2021

In this paper, we propose random beam-based non-orthogonal multiple access (NOMA) for low latency multiple-input single-output (MISO) broadcast channels, where there is a target signal-to-interference-plus-noise power ratio (SINR) for each user. In our system model, there is a multi-antenna transmitter with its own single antenna users, and the transmitter selects and serves some of them. For low latency, the transmitter exploits random beams, which can reduce the feedback overhead for the channel acquisition, and each beam can support more than a single user with NOMA. In our proposed random beam-based NOMA, each user feeds a selected beam index, the corresponding SINR, and the channel gain, so it feeds one more scalar value compared to the conventional random beamforming. By allocating the same powers across the beams, the transmitter independently selects NOMA users for each beam, so it can also reduce the computational complexity. We optimize our proposed scheme finding the optimal user grouping and the optimal power allocation. The numerical results show that our proposed scheme outperforms the conventional random beamforming by supporting more users for each beam.