bluuebunny/crossref_metadata_2025 huggingface dataset:
Interstellar medium properties and feedback in local AGN with the MAGNUM survey
Authors: Mingozzi M. , Cresci G. , Venturi G. , Marconi A. , Mannucci F.
Published: March 2021

We investigated the interstellar medium (ISM) properties in the central regions of nearby Seyfert galaxies characterised by prominent conical or bi-conical outflows belonging to the MAGNUM survey by exploiting the unprecedented sensitivity, spatial and spectral coverage of the integral field spectrograph MUSE at the Very Large Telescope. We developed a novel approach based on the gas and stars kinematics to disentangle high-velocity gas in the outflow from gas in the disc to spatially track the differences in their ISM properties. This allowed us to reveal the presence of an ionisation structure within the extended outflows that can be interpreted with different photoionisation and shock conditions, and to trace tentative evidence of outflow-induced star formation (“positive” feedback) in a galaxy of the sample, Centaurus A.
Cosmic magnetism evolution using cosmological simulations
Authors: Adduci Faria Stela , de Gouveia Dal Pino Elisabete M. , Barai Paramita
Published: March 2021

The Intergalactic Medium (IGM) is the region comprising the environment between the galaxies. Gamma-ray observations have provided lower limits to IGM magnetic fields of the order of ≳10–16 G. Magnetic fields are continuously ejected from galaxies by jets and galactic winds. However, the origin and evolution of cosmic magnetic fields in the more diffuse regions, like voids, is still debated. The difficulties in directly measuring magnetic fields and their coherent scales, make hydrodynamic and magnetohydrodynamic (MHD) cosmological simulations useful tools to shed light on this debate. As a first approach, we have performed hydrodynamic cosmological simulations assuming energy equipartition as an initial condition between the baryonic gas and the magnetic field, starting at z = 8, to track the evolution of magnetic fields, and compare with results of MHD simulations. We have found that for halos and cores, our results are comparable to the MHD description. For the less dense regions, the equipartition condition clearly overestimates the observed limits. In forthcoming work, we will investigate MHD simulations of cosmological evolution and amplification of seed magnetic fields, considering all relevant feedback processes and exploring turbulent dynamo amplification versus primordial mechanisms across cosmological timescales.
Tracing young SMBHs in the dusty distant universe – a Chandra view of DOGs
Authors: Menéndez-Delmestre Karín , Riguccini Laurie , Treister Ezequiel
Published: March 2021

The coexistence of star formation and AGN activity has geared much attention to dusty galaxies at high redshifts, in the interest of understanding the origin of the Magorrian relation observed locally, where the mass of the stellar bulk in a galaxy appears to be tied to the mass of the underlying supermassive black hole. We exploit the combined use of far-infrared (IR) Herschel data and deep Chandra ˜160 ksec depth X-ray imaging of the COSMOS field to probe for AGN signatures in a large sample of >100 Dust-Obscured Galaxies (DOGs). Only a handful (˜20%) present individual X-ray detections pointing to the presence of significant AGN activity, while X-ray stacking analysis on the X-ray undetected DOGs points to a mix between AGN activity and star formation. Together, they are typically found on the main sequence of star-forming galaxies or below it, suggesting that they are either still undergoing significant build up of the stellar bulk or have started quenching. We find only ˜30% (6) Compton-thick AGN candidates (NH > 1024 cm–2), which is the same frequency found within other soft- and hard-X-ray selected AGN populations. This suggests that the large column densities responsible for the obscuration in Compton-thick AGNs must be nuclear and have little to do with the dust obscuration of the host galaxy. We find that DOGs identified to have an AGN share similar near-IR and mid-to-far-IR colors, independently of whether they are individually detected or not in the X-ray. The main difference between the X-ray detected and the X-ray undetected populations appears to be in their redshift distributions, with the X-ray undetected ones being typically found at larger distances. This strongly underlines the critical need for multiwavelength studies in order to obtain a more complete census of the obscured AGN population out to higher redshifts. For more details, we refer the reader to Riguccini et al. (2019).
Stellar population synthesis of jellyfish galaxies
Authors: Azevedo Gabriel M. , Chies Santos Ana L. , Riffel Rogério , Lassen Augusto , Trevisan Marina , Mallmann Nícolas , Oliveira Fernanda , Gomes Jean
Published: March 2021

Jellyfish are the most extreme cases of galaxies undergoing ram-pressure stripping. In order to analyse the stellar populations distribution along these galaxies, we have performed stellar population synthesis in data cubes of jellyfish from the GASP programme, using both Starlight and FADO codes.
GOODS-ALMA: AGNs and the slow downfall of massive star-forming galaxies at z > 2
Authors: Franco Maximilien
Published: March 2021

We present the results of a 69 arcmin2 ALMA survey at 1.1 mm, GOODS-ALMA, matching the deepest HST-WFC3 H-band observed region of the GOODS-South field. The 35 galaxies detected by ALMA are among the most massive galaxies at z = 2–4 and are either starburst or located in the upper part of the galaxy star-forming main sequence. The analysis of the gas fraction, depletion time, X-ray luminosity and the size suggests that they are building compact bulges and are the ideal progenitors of compact passive galaxies at z˜2, and a slow downfall scenario is favoured in their future transition from star-forming to passive galaxies.
Cold gas studies of a z = 2.5 protocluster
Authors: Lee Minju M. , Tanaka Ichi , Kawabe Rohei
Published: March 2021

We present studies of a protocluster at z =2.5, an overdense region found close to a radio galaxy, 4C 23.56, using ALMA. We observed 1.1 mm continuum, two CO lines (CO (4–3) and CO (3–2)) and the lower atomic carbon line transition ([CI](3P1-3P0)) at a few kpc (0″.3-0″.9) resolution. The primary targets are 25 star-forming galaxies selected as Hα emitters (HAEs) that are identified with a narrow band filter. These are massive galaxies with stellar masses of > 1010Mʘ that are mostly on the galaxy main sequence at z =2.5. We measure the molecular gas mass from the independent gas tracers of 1.1 mm, CO (3–2) and [CI], and investigate the gas kinematics of galaxies from CO (4–3). Molecular gas masses from the different measurements are consistent with each other for detection, with a gas fraction (fgas = Mgas/(Mgas+ Mstar)) of ≃ 0.5 on average but with a caveat. On the other hand, the CO line widths of the protocluster galaxies are typically broader by ˜50% compared to field galaxies, which can be attributed to more frequent, unresolved gas-rich mergers and/or smaller sizes than field galaxies, supported by our high-resolution images and a kinematic model fit of one of the galaxies. We discuss the expected scenario of galaxy evolution in protoclusters at high redshift but future large surveys are needed to get a more general view.
Nature of inflows and outflows in AGNs
Authors: Wada Keiichi , Kudoh Yuki , Yutani Naomichi , Kawakatu Nozomu
Published: March 2021

Despite many theoretical studies and observations, we still do not fully understand the feeding mechanism in AGNs even in nearby galaxies, and how feedback from AGNs affects the gas dynamics itself in the galactic central regions. In this article, we summarize our recent theoretical studies and preliminary results in terms of the mass inflow and outflows on sub-parsec to 100 parsecs scales around AGNs. We introduce different studies: 1) How do galaxy-galaxy mergers trigger AGN activity and obscuration?, 2) How do the radiative feedback affect formation of outflows and obscuration of the nucleus? and 3) How does the AGN plus starburst feedback contribute to the obscuration?
Ambulante Behandlung nach Spitalaustritt
Authors: Thorn David Roger , Ladewig Hess Ariane Ruth
Published: March 2021

Zusammenfassung. Nach der operativen Behandlung des Mammakarzinoms folgt in der Regel eine längerfristige medikamentöse Therapie sowie anschliessend oder zeitgleich eine mehrjährige ambulante Tumornachsorge in der Praxis. Diese medikamentösen Therapien, welche das Rezidivrisiko des Mammakarzinoms nachweislich senken und in ihrer Summe zu einer erheblichen Verbesserung der Prognose dieser Erkrankung geführt haben, werden im folgenden Artikel anhand aktuellster Empfehlungen internationaler Fachgesellschaften unter Berücksichtigung der verschiedenen Tumorbiologien und Subtypen (luminal, HER2-positiv, triple-negativ / basal) entsprechend erläutert. Ein besonderer Fokus wird hierbei auch auf die Bewältigung der häufig auftretenden Ängste und praxisrelevanten, therapieassoziierten Nebenwirkungen gelegt. Abschliessend beschreiben wir aus unserer Perspektive die Grundzüge einer erfolgreichen interdisziplinären Mammakarzinom-Nachsorge nach heutigem Standard.
Integrative Onkologie in der Behandlung von Frauen mit Brustkrebs
Authors: Kienle Gunver Sophia , Rist Lukas
Published: March 2021

Zusammenfassung. Die integrative Onkologie wird heute weltweit ergänzend zur onkologischen Standardtherapie eingesetzt und von den Patienten aktiv gesucht und ist auch in der Schweiz etabliert. Vielfältige Verfahren kommen zum Einsatz, wie Mind-Body Medizin, Kunsttherapien, Bewegung, Ernährung, pflanzliche Arzneimittel, Pflegemassnahmen und spezifische komplementärmedizinische Verfahren. Die meisten sind wissenschaftlich weitreichend untersucht und ihre Anwendung qualitätsgesichert. Sie unterstützen die Patienten bei der Genesung, verhelfen zu einer besseren Lebensqualität und ermöglichen ihnen, selber aktiv etwas zu ihrer Gesundheit und Behandlung beizutragen. Eine Evidenz-basierte Leitlinie zur integrativmedizinischen Behandlung von Brustkrebs wurde von der Society of Integrative Oncology publiziert und von der American Society of Clinical Oncology implementiert. Weithin angewendet wird auch die Misteltherapie, die ebenfalls zu einer Verbesserung der Lebensqualität beitragen kann und deren Wirkweise, Sicherheit und Wirksamkeit intensiv untersucht wird. Zentral ist das vertrauensvolle und wertschätzende Gespräch, damit aus der Fülle an Informationen und Massnahmen ein individuell sinnvolles und sicheres Behandlungskonzept entstehen kann.
Modeling Lunar Pyroclasts to Probe the Volatile Content of the Lunar Interior
Authors: Florez Darien , Huber Christian , Milliken Ralph E. , Berkson Julia
Published: March 2021

Constraining the volatile budget of the lunar interior has important ramifications for models of Moon formation. While many early and previous measurements of samples acquired from the Luna and Apollo missions suggested the lunar interior is depleted in highly volatile elements like H, a number of high‐precision analytical studies over the past decade have argued that it may be more enriched in water than previously thought. Here, we integrate recent remotely sensed near‐infrared reflectance measurements of small Dark‐Mantle‐Deposits (DMDs) Birt E and Grimaldi, interpreted to represent pyroclastic deposits, and physics‐based eruption models to better constrain the preeruptive water content of the magmas that resulted in these deposits. We model the trajectory and water loss of pyroclasts from eruption to deposition, coupling eruption dynamics with a volatile diffusion model for each pyroclast. Modeled pyroclast sizes and final water contents are then used to predict spectral reflectance properties for comparison with the observed orbital near‐infrared data. We develop an inversion scheme based on the Markov‐Chain Monte‐Carlo (MCMC) method to retrieve constraints between governing parameters such as the initial volatile content of the melt and the pyroclast size distribution (which influences the remotely measured water absorption strengths). The MCMC inversion allows us to estimate the primordial (preeruption) water content for different DMDs and therefore explore whether their source is volatile‐rich. Our results suggest that the preeruptive water content of the magmas sampled by Birt E and Grimaldi can be constrained within a range 400–800 ppm, while the pyroclast size in diameter corresponding to the 50th percentile of a given deposit likely ranges from ∼400 to 600 μm in diameter. Finally, we determine the evaporation and cooling rates are likely low, ∼10−6 m/s and 6°C/s, respectively.
Mass Bird Die-Off Linked to Wildfires and Toxic Gases
Authors: Learn Joshua
Published: March 2021

Using observations from crowdsourced science and weather location data, researchers concluded that wildfires caused a mass die-off of birds in the western and central United States in 2020.
The temperature sensitivity of soil: microbial biodiversity, growth, and carbon mineralization
Authors: Wang Chao , Morrissey Ember M , Mau Rebecca L , Hayer Michaela , Piñeiro Juan , Mack Michelle C , Marks Jane C , Bell Sheryl L , Miller Samantha N , Schwartz Egbert , Dijkstra Paul , Koch Benjamin J , Stone Bram W , Purcell Alicia M , Blazewicz Steven J , Hofmockel Kirsten S , Pett-Ridge Jennifer , Hungate Bruce A
Published: March 2021

Microorganisms drive soil carbon mineralization and changes in their activity with increased temperature could feedback to climate change. Variation in microbial biodiversity and the temperature sensitivities (Q10) of individual taxa may explain differences in the Q10 of soil respiration, a possibility not previously examined due to methodological limitations. Here, we show phylogenetic and taxonomic variation in the Q10 of growth (5–35 °C) among soil bacteria from four sites, one from each of Arctic, boreal, temperate, and tropical biomes. Differences in the temperature sensitivities of taxa and the taxonomic composition of communities determined community-assembled bacterial growth Q10, which was strongly predictive of soil respiration Q10 within and across biomes. Our results suggest community-assembled traits of microbial taxa may enable enhanced prediction of carbon cycling feedbacks to climate change in ecosystems across the globe.
Polygenic risk score, healthy lifestyles, and risk of incident depression
Authors: Cao Zhi , Yang Hongxi , Ye Yixuan , Zhang Yuan , Li Shu , Zhao Hongyu , Wang Yaogang
Published: March 2021

Genetic factors increase the risk of depression, but the extent to which this can be offset by modifiable lifestyle factors is unknown. We investigated whether a combination of healthy lifestyles is associated with lower risk of depression regardless of genetic risk. Data were obtained from the UK Biobank and consisted of 339,767 participants (37–73 years old) without depression between 2006 and 2010. Genetic risk was categorized as low, intermediate, or high according to polygenic risk score for depression. A combination of healthy lifestyles factors—including no current smoking, regular physical activity, a healthy diet, moderate alcohol intake and a body mass index <30 kg/m2—was categorized into favorable, intermediate, and unfavorable lifestyles. The risk of depression was 22% higher among those at high genetic risk compared with those at low genetic risk (HR = 1.22, 95% CI: 1.14–1.30). Participants with high genetic risk and unfavorable lifestyle had a more than two-fold risk of incident depression compared with low genetic risk and favorable lifestyle (HR = 2.18, 95% CI: 1.84–2.58). There was no significant interaction between genetic risk and lifestyle factors (P for interaction = 0.69). Among participants at high genetic risk, a favorable lifestyle was associated with nearly 50% lower relative risk of depression than an unfavorable lifestyle (HR = 0.51, 95% CI: 0.43–0.60). We concluded that genetic and lifestyle factors were independently associated with risk of incident depression. Adherence to healthy lifestyles may lower the risk of depression regardless of genetic risk.
A randomised, double-blind, sham-controlled trial of deep brain stimulation of the bed nucleus of the stria terminalis for treatment-resistant obsessive-compulsive disorder
Authors: Mosley Philip E. , Windels François , Morris John , Coyne Terry , Marsh Rodney , Giorni Andrea , Mohan Adith , Sachdev Perminder , O’Leary Emily , Boschen Mark , Sah Pankaj , Silburn Peter A.
Published: March 2021

Deep brain stimulation (DBS) is a promising treatment for severe, treatment-resistant obsessive-compulsive disorder (OCD). Here, nine participants (four females, mean age 47.9 ± 10.7 years) were implanted with DBS electrodes bilaterally in the bed nucleus of the stria terminalis (BNST). Following a one-month postoperative recovery phase, participants entered a three-month randomised, double-blind, sham-controlled phase before a twelve-month period of open-label stimulation incorporating a course of cognitive behavioural therapy (CBT). The primary outcome measure was OCD symptoms as rated with the Yale-Brown Obsessive-Compulsive Scale (YBOCS). In the blinded phase, there was a significant benefit of active stimulation over sham (p = 0.025, mean difference 4.9 points). After the open phase, the mean reduction in YBOCS was 16.6 ± 1.9 points (χ2 (11) = 39.8, p = 3.8 × 10−5), with seven participants classified as responders. CBT resulted in an additive YBOCS reduction of 4.8 ± 3.9 points (p = 0.011). There were two serious adverse events related to the DBS device, the most severe of which was an infection during the open phase necessitating device explantation. There were no serious psychiatric adverse events related to stimulation. An analysis of the structural connectivity of each participant’s individualised stimulation field isolated right-hemispheric fibres associated with YBOCS reduction. These included subcortical tracts incorporating the amygdala, hippocampus and stria terminalis, in addition to cortical regions in the ventrolateral and ventromedial prefrontal cortex, parahippocampal, parietal and extrastriate visual cortex. In conclusion, this study provides further evidence supporting the efficacy and tolerability of DBS in the region of the BNST for individuals with otherwise treatment-refractory OCD and identifies a connectivity fingerprint associated with clinical benefit.
Promises and challenges of adoptive T-cell therapies for solid tumours
Authors: Morotti Matteo , Albukhari Ashwag , Alsaadi Abdulkhaliq , Artibani Mara , Brenton James D. , Curbishley Stuart M. , Dong Tao , Dustin Michael L. , Hu Zhiyuan , McGranahan Nicholas , Miller Martin L. , Santana-Gonzalez Laura , Seymour Leonard W. , Shi Tingyan , Van Loo Peter , Yau Christopher , White Helen , Wietek Nina , Church David N. , Wedge David C. , Ahmed Ahmed A.
Published: March 2021

Cancer is a leading cause of death worldwide and, despite new targeted therapies and immunotherapies, many patients with advanced-stage- or high-risk cancers still die, owing to metastatic disease. Adoptive T-cell therapy, involving the autologous or allogeneic transplant of tumour-infiltrating lymphocytes or genetically modified T cells expressing novel T-cell receptors or chimeric antigen receptors, has shown promise in the treatment of cancer patients, leading to durable responses and, in some cases, cure. Technological advances in genomics, computational biology, immunology and cell manufacturing have brought the aspiration of individualised therapies for cancer patients closer to reality. This new era of cell-based individualised therapeutics challenges the traditional standards of therapeutic interventions and provides opportunities for a paradigm shift in our approach to cancer therapy. Invited speakers at a 2020 symposium discussed three areas—cancer genomics, cancer immunology and cell-therapy manufacturing—that are essential to the effective translation of T-cell therapies in the treatment of solid malignancies. Key advances have been made in understanding genetic intratumour heterogeneity, and strategies to accurately identify neoantigens, overcome T-cell exhaustion and circumvent tumour immunosuppression after cell-therapy infusion are being developed. Advances are being made in cell-manufacturing approaches that have the potential to establish cell-therapies as credible therapeutic options. T-cell therapies face many challenges but hold great promise for improving clinical outcomes for patients with solid tumours.
Whole exome sequencing reveals putatively novel associations in retinopathies and drusen formation
Authors: Doucette Lance P. , Noel Nicole C. L. , Zhai Yi , Xu Manlong , Caluseriu Oana , Hoang Stephanie C. , Radziwon Alina J. , MacDonald Ian M.
Published: March 2021

Inherited retinal dystrophies (IRDs) affect 1 in 3000 individuals worldwide and are genetically heterogeneous, with over 270 identified genes and loci; however, there are still many identified disorders with no current genetic etiology. Whole exome sequencing (WES) provides a hypothesis-free first examination of IRD patients in either a clinical or research setting to identify the genetic cause of disease. We present a study of IRD in ten families from Alberta, Canada, through the lens of novel gene discovery. We identify the genetic etiology of IRDs in three of the families to be variants in known disease-associated genes, previously missed by clinical investigations. In addition, we identify two potentially novel associations: LRP1 in early-onset drusen formation and UBE2U in a multi-system condition presenting with retinoschisis, cataracts, learning disabilities, and developmental delay. We also describe interesting results in our unsolved cases to provide further information to other investigators of these blinding conditions.
Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor
Authors: Attia Jessica , Nir Sivan , Mervinetsky Evgeniy , Balogh Dora , Gitlin-Domagalska Agata , Alshanski Israel , Reches Meital , Hurevich Mattan , Yitzchaik Shlomo
Published: March 2021

Peptides are commonly used as biosensors for analytes such as metal ions as they have natural binding preferences. In our previous peptide-based impedimetric metal ion biosensors, a monolayer of the peptide was anchored covalently to the electrode. Binding of metal ions resulted in a conformational change of the oxytocin peptide in the monolayer, which was measured using electrochemical impedance spectroscopy. Here, we demonstrate that sensing can be achieved also when the oxytocin is non-covalently integrated into an alkanethiol host monolayer. We show that ion-binding cause morphological changes to the dense host layer, which translates into enhanced impedimetric signals compared to direct covalent assembly strategies. This biosensor proved selective and sensitive for Zn2+ ions in the range of nano- to micro-molar concentrations. This strategy offers an approach to utilize peptide flexibility in monitoring their response to the environment while embedded in a hydrophobic monolayer.
An insight into the estimation of drilling fluid density at HPHT condition using PSO-, ICA-, and GA-LSSVM strategies
Authors: Alizadeh S. M. , Alruyemi Issam , Daneshfar Reza , Mohammadi-Khanaposhtani Mohammad , Naseri Maryam
Published: March 2021

The present study evaluates the drilling fluid density of oil fields at enhanced temperatures and pressures. The main objective of this work is to introduce a set of modeling and experimental techniques for forecasting the drilling fluid density via various intelligent models. Three models were assessed, including PSO-LSSVM, ICA-LSSVM, and GA-LSSVM. The PSO-LSSVM technique outperformed the other models in light of the smallest deviation factor, reflecting the responses of the largest accuracy. The experimental and modeled regression diagrams of the coefficient of determination (R2) were plotted. In the GA-LSSVM approach, R2was calculated to be 0.998, 0.996 and 0.996 for the training, testing and validation datasets, respectively. R2was obtained to be 0.999, 0.999 and 0.998 for the training, testing and validation datasets, respectively, in the ICA-LSSVM approach. Finally, it was found to be 0.999, 0.999 and 0.999 for the training, testing and validation datasets in the PSO-LSSVM method, respectively. In addition, a sensitivity analysis was performed to explore the impacts of several variables. It was observed that the initial density had the largest impact on the drilling fluid density, yielding a 0.98 relevancy factor.
Ibuprofen alters epoxide hydrolase activity and epoxy-oxylipin metabolites associated with different metabolic pathways in murine livers
Authors: Tiwari Shuchita , Yang Jun , Morisseau Christophe , Durbin-Johnson Blythe , Hammock Bruce D. , Gomes Aldrin V.
Published: March 2021

Over the last decade oxylipins have become more recognized for their involvement in several diseases. Non-steroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen are known to inhibit cyclooxygenase (COX) enzymes, but how NSAIDs affect oxylipins, in addition to COX products, in animal tissues is not well understood. Oxylipins in livers from male and female mice treated with 100 mg/kg/day of ibuprofen for 7 days were investigated. The results showed that ibuprofen treated male livers contained 7 times more altered oxylipins than ibuprofen treated female livers. In male and female livers some prostaglandins were altered, while diols, hydroxy fatty acids and epoxides were significantly altered in male livers. Some soluble epoxide hydrolase (sEH) products, such as 9,10-DiHODE were found to be decreased, while sEH substrates (such as 9(10)-EpODE and 5(6)-EpETrE) were found to be increased in male livers treated with ibuprofen, but not in ibuprofen treated female livers. The enzymatic activities of sEH and microsomal epoxide hydrolase (mEH) were elevated by ibuprofen in both males and females. Analyzing the influence of sex on the effect of ibuprofen on oxylipins and COX products showed that approximately 27% of oxylipins detected were influenced by sex. The results reveal that ibuprofen disturbs not only the COX pathway, but also the CYP450 and lipoxygenase pathways in male mice, suggesting that ibuprofen is likely to generate sex related differences in biologically active oxylipins. Increased sEH activity after ibuprofen treatment is likely to be one of the mechanisms by which the liver reduces the higher levels of EpODEs and EpETrEs.
A novel method for noninvasive bioelectric measurement utilizing conductivity of seawater
Authors: Saiki Tsunemasa , Takizawa Yukako , Murai Koji , Okuno Ryuhei , Arima Masakazu
Published: March 2021

A novel method of noninvasive bioelectric measurement that utilizes the conductivity of seawater covering a person’s whole body is proposed. Concretely, a conductor used as a common electrode is sunk into the seawater, and four special bioelectrodes isolated from the seawater are attached at measurement points on the body. Bioelectric signals generated between the common electrode and special bioelectrodes are then measured. To verify the effectiveness of the proposed method, bioelectric signals of six participants immersed in a bathtub filled with seawater were experimentally measured. The measurement results revealed that the proposed method enables multipoint bioelectric measurement using about half the number of bioelectrodes used by the conventional method on land, and a plurality of bioelectric phenomena can be observed at one measurement point. It was also revealed that compared with the conventional method, the proposed method significantly reduces external electrical noise included in the bioelectric signals by exploiting the shielding effect of seawater. If simple bioelectric measurements in seawater were possible in the manner described above, not only people such as scuba divers but also precious animals living in the sea could be noninvasively treated as measurement subjects.
Emerging strains of watermelon mosaic virus in Southeastern France: model-based estimation of the dates and places of introduction
Authors: Roques L. , Desbiez C. , Berthier K. , Soubeyrand S. , Walker E. , Klein E. K. , Garnier J. , Moury B. , Papaïx J.
Published: March 2021

Where and when alien organisms are successfully introduced are central questions to elucidate biotic and abiotic conditions favorable to the introduction, establishment and spread of invasive species. We propose a modelling framework to analyze multiple introductions by several invasive genotypes or genetic variants, in competition with a resident population, when observations provide knowledge on the relative proportions of each variant at some dates and places. This framework is based on a mechanistic-statistical model coupling a reaction–diffusion model with a probabilistic observation model. We apply it to a spatio-temporal dataset reporting the relative proportions of five genetic variants of watermelon mosaic virus (WMV, genusPotyvirus, familyPotyviridae) in infections of commercial cucurbit fields. Despite the parsimonious nature of the model, it succeeds in fitting the data well and provides an estimation of the dates and places of successful introduction of each emerging variant as well as a reconstruction of the dynamics of each variant since its introduction.
The relative deficit of GDF15 in adolescent girls with PCOS can be changed into an abundance that reduces liver fat
Authors: de Zegher Francis , Díaz Marta , Villarroya Joan , Cairó Montserrat , López-Bermejo Abel , Villarroya Francesc , Ibáñez Lourdes
Published: March 2021

A prime concern of young patients with Polycystic Ovary Syndrome (PCOS) is the control of body adiposity, given their tendency to gain weight and/or their difficulty to lose weight. Circulating growth-and-differentiation factor-15 (GDF15) facilitates the control of body weight via receptors in the brainstem. C-reactive protein (CRP) and insulin are endogenous GDF15 secretagogues. We hypothesised that PCOS in non-obese adolescents is characterised by low concentrations of circulating GDF15, when judged by the degree of CRP and insulin drive. GDF15 was added as a post-hoc endpoint of two previously reported, randomised studies in non-obese adolescent girls with PCOS (N = 58; 60% normal weight; 40% overweight) who received either an oral oestroprogestogen contraceptive (OC), or a low-dose combination of spironolactone-pioglitazone-metformin (SPIOMET) for 1 year; subsequently, all girls remained untreated for 1 year. Adolescent girls with regular menses (N = 20) served as healthy controls. Circulating GDF15, CRP and fasting insulin were assessed prior to treatment, and halfway the on- and post-treatment years. Pre-treatment, the absolute GDF15 concentrations were normal in PCOS girls, but their relative levels were markedly low, in view of the augmented CRP and insulin drives. OC treatment was accompanied by a near-doubling of circulating GDF15 (on average, from 296 to 507 pg/mL) and CRP, so that the relative GDF15 levels remained low. SPIOMET treatment was accompanied by a 3.4-fold rise of circulating GDF15 (on average, from 308 to 1045 pg/mL) and by a concomitant lowering of CRP and insulin concentrations towards normal, so that the relative GDF15 levels became markedly abundant. Post-OC, the relatively low GDF15 levels persisted; post-SPIOMET, the circulating concentrations of GDF15, CRP and insulin were all normal. BMI remained stable in both treatment groups. Only SPIOMET was accompanied by a reduction of hepato-visceral fat (by MRI) towards normal. In conclusion, early PCOS was found to be characterised by a relative GDF15 deficit that may partly explain the difficulties that young patients experience to control their body adiposity. This relative GDF15 deficit persisted during and after OC treatment. In contrast, SPIOMET treatment was accompanied by an absolute and a relative abundance of GDF15, and followed by normal GDF15, CRP and insulin concentrations. The present findings strengthen the rationale to raise the concentrations of circulating GDF15 in early PCOS, for example with a SPIOMET-like intervention that attenuates low-grade inflammation, insulin resistance and ectopic adiposity, without necessarily lowering body weight.

Clinical trial registries: ISRCTN29234515 and ISRCTN11062950.
Emotion detection using electroencephalography signals and a zero-time windowing-based epoch estimation and relevant electrode identification
Authors: Gannouni Sofien , Aledaily Arwa , Belwafi Kais , Aboalsamh Hatim
Published: March 2021

Recognizing emotions using biological brain signals requires accurate and efficient signal processing and feature extraction methods. Existing methods use several techniques to extract useful features from a fixed number of electroencephalography (EEG) channels. The primary objective of this study was to improve the performance of emotion recognition using brain signals by applying a novel and adaptive channel selection method that acknowledges that brain activity has a unique behavior that differs from one person to another and one emotional state to another. Moreover, we propose identifying epochs, which are the instants at which excitation is maximum, during the emotion to improve the system’s accuracy. We used the zero-time windowing method to extract instantaneous spectral information using the numerator group-delay function to accurately detect the epochs in each emotional state. Different classification scheme were defined using QDC and RNN and evaluated using the DEAP database. The experimental results showed that the proposed method is highly competitive compared with existing studies of multi-class emotion recognition. The average accuracy rate exceeded 89%. Compared with existing algorithms dealing with 9 emotions, the proposed method enhanced the accuracy rate by 8%. Moreover, experiment shows that the proposed system outperforms similar approaches discriminating between 3 and 4 emotions only. We also found that the proposed method works well, even when applying conventional classification algorithms.
Reliability assessment of temporal discounting measures in virtual reality environments
Authors: Bruder Luca R. , Scharer Lisa , Peters Jan
Published: March 2021

In recent years the emergence of high-performance virtual reality (VR) technology has opened up new possibilities for the examination of context effects in psychological studies. The opportunity to create ecologically valid stimulation in a highly controlled lab environment is especially relevant for studies of psychiatric disorders, where it can be problematic to confront participants with certain stimuli in real life. However, before VR can be confidently applied widely it is important to establish that commonly used behavioral tasks generate reliable data within a VR surrounding. One field of research that could benefit greatly from VR-applications are studies assessing the reactivity to addiction related cues (cue-reactivity) in participants suffering from gambling disorder. Here we tested the reliability of a commonly used temporal discounting task in a novel VR set-up designed for the concurrent assessment of behavioral and psychophysiological cue-reactivity in gambling disorder. On 2 days, thirty-four healthy non-gambling participants explored two rich and navigable VR-environments (neutral: café vs. gambling-related: casino and sports-betting facility), while their electrodermal activity was measured using remote sensors. In addition, participants completed the temporal discounting task implemented in each VR environment. On a third day, participants performed the task in a standard lab testing context. We then used comprehensive computational modeling using both standard softmax and drift diffusion model (DDM) choice rules to assess the reliability of discounting model parameters assessed in VR. Test–retest reliability estimates were good to excellent for the discount rate log(k), whereas they were poor to moderate for additional DDM parameters. Differences in model parameters between standard lab testing and VR, reflecting reactivity to the different environments, were mostly numerically small and of inconclusive directionality. Finally, while exposure to VR generally increased tonic skin conductance, this effect was not modulated by the neutral versus gambling-related VR-environment. Taken together this proof-of-concept study in non-gambling participants demonstrates that temporal discounting measures obtained in VR are reliable, suggesting that VR is a promising tool for applications in computational psychiatry, including studies on cue-reactivity in addiction.
Beneficial effects of whole-body cryotherapy on glucose homeostasis and amino acid profile are associated with a reduced myostatin serum concentration
Authors: Kozłowska Marta , Kortas Jakub , Żychowska Małgorzata , Antosiewicz Jędrzej , Żuczek Klaudia , Perego Silvia , Lombardi Giovanni , Ziemann Ewa
Published: March 2021

The study investigated the effect of single and chronic (10 sessions) whole-body cryotherapy (WBC; 3-min, − 110 °C) on amino acid (AA) profile, myostatin, fibroblast growth factor 21 (FGF21), and concentrations of brain-derived neurotrophic factor (BDNF), irisin and adiponectin in relation to glucose homeostasis. Thirty-five, healthy men were randomly split into experimental (young: 28 ± 7 years and middle-aged: 51 ± 3 years) and control groups. Blood samples were taken before and 1 h after the first and last (10th) WBC session. Baseline myostatin correlated significantly with visceral fat area, glucose, insulin, HOMA-IR and irisin (all p < 0.05). The single session of WBC induced temporary changes in AA profile, whereas chronic exposure lowered valine and asparagine concentrations (p < 0.01 and p = 0.01, respectively) compared to the baseline. The chronic WBC reduced fasting glucose (p = 0.04), FGF21 (− 35.8%, p = 0.06) and myostatin (-18.2%, p = 0.06). Still, the effects were age-dependent. The decrease of myostatin was more pronounced in middle-aged participants (p < 0.01). Concentrations of irisin and adiponectin increased in response to chronic WBC, while BDNF level remained unchanged. By improving the adipo-myokine profile, chronic WBC may reduce effectively the risk of the metabolic syndrome associated with hyperinsulinemia, increased levels of valine and asparagine, and muscle atrophy.
Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
Authors: Thiam Laty G. , Nyarko Prince B. , Kusi Kwadwo A. , Niang Makhtar , Aniweh Yaw , Awandare Gordon A.
Published: March 2021

Human erythrocytes are indispensable for Plasmodium falciparum development. Unlike other eukaryotic cells, there is no existing erythroid cell line capable of supporting long-term P. falciparum in vitro experiments. Consequently, invasion phenotyping experiments rely on erythrocytes of different individuals. However, the contribution of the erythrocytes variation in influencing invasion rates remains unknown, which represents a challenge for conducting large-scale comparative studies. Here, we used erythrocytes of different blood groups harboring different hemoglobin genotypes to assess the relative contribution of blood donor variability in P. falciparum invasion phenotyping assays. For each donor, we investigated the relationship between parasite invasion phenotypes and erythrocyte phenotypic characteristics, including the expression levels of surface receptors (e.g. the human glycophorins A and C, the complement receptor 1 and decay accelerating factor), blood groups (e.g. ABO/Rh system), and hemoglobin genotypes (e.g. AA, AS and AC). Across all donors, there were significant differences in invasion efficiency following treatment with either neuraminidase, trypsin or chymotrypsin relative to the control erythrocytes. Primarily, we showed that the levels of key erythrocyte surface receptors and their sensitivity to enzyme treatment significantly differed across donors. However, invasion efficiency did not correlate with susceptibility to enzyme treatment or with the levels of the selected erythrocyte surface receptors. Furthermore, we found no relationship between P. falciparum invasion phenotype and blood group or hemoglobin genotype. Altogether, our findings demonstrate the need to consider erythrocyte donor uniformity and anticipate challenges associated with blood donor variability in early stages of large-scale study design.
The effect of low-temperature annealing on discordance of U–Pb zircon ages
Authors: Herrmann Maria , Söderlund Ulf , Scherstén Anders , Næraa Tomas , Holm-Alwmark Sanna , Alwmark Carl
Published: March 2021

Discordant U–Pb data of zircon are commonly attributed to Pb loss from domains with variable degree of radiation damage that resulted from α-decay of U and Th, which often complicates the correct age interpretation of the sample. Here we present U–Pb zircon data from 23 samples of ca. 1.7–1.9 Ga granitoid rocks in and around the Siljan impact structure in central Sweden. Our results show that zircon from rocks within the structure that form an uplifted central plateau lost significantly less radiogenic Pb compared to zircon grains in rocks outside the plateau. We hypothesize that zircon in rocks within the central plateau remained crystalline through continuous annealing of crystal structure damages induced from decay of U and Th until uplifted to the surface by the impact event ca. 380 Ma ago. In contrast, zircon grains distal to the impact have accumulated radiation damage at shallow and cool conditions since at least 1.26 Ga, making them vulnerable to fluid-induced Pb-loss. Our data are consistent with studies on alpha recoil and fission tracks, showing that annealing in zircon occurs at temperatures as low as 200–250 °C. Zircon grains from these samples are texturally simple, i.e., neither xenocrysts nor metamorphic overgrowths have been observed. Therefore, the lower intercepts obtained from regression of variably discordant zircon data are more likely recording the age of fluid-assisted Pb-loss from radiation-damaged zircon at shallow levels rather than linked to regional magmatic or tectonic events.
Thermal expansion coefficient of few-layer MoS2 studied by temperature-dependent Raman spectroscopy
Authors: Lin Zhongtao , Liu Wuguo , Tian Shibing , Zhu Ke , Huang Yuan , Yang Yang
Published: March 2021

The thermal expansion coefficient is an important thermal parameter that influences the performance of nanodevices based on two-dimensional materials. To obtain the thermal expansion coefficient of few-layer MoS2, suspended MoS2 and supported MoS2 were systematically investigated using Raman spectroscopy in the temperature range from 77 to 557 K. The temperature-dependent evolution of the Raman frequency shift for suspended MoS2 exhibited prominent differences from that for supported MoS2, obviously demonstrating the effect due to the thermal expansion coefficient mismatch between MoS2 and the substrate. The intrinsic thermal expansion coefficients of MoS2 with different numbers of layers were calculated. Interestingly, negative thermal expansion coefficients were obtained below 175 K, which was attributed to the bending vibrations in the MoS2 layer during cooling. Our results demonstrate that Raman spectroscopy is a feasible tool for investigating the thermal properties of few-layer MoS2 and will provide useful information for its further application in photoelectronic devices.
In vitro skin culture media influence the viability and inflammatory response of primary macrophages
Authors: Griffoni Chiara , Neidhart Berna , Yang Ke , Groeber-Becker Florian , Maniura-Weber Katharina , Dandekar Thomas , Walles Heike , Rottmar Markus
Published: March 2021

The replacement of animal models for investigation of inflammation and wound healing has been advancing by means of in vitro skin equivalents with increasing levels of complexity. However, the current in vitro skin models still have a limited pre-clinical relevance due to their lack of immune cells. So far, few steps have been made towards the incorporation of immune cells into in vitro skin and the requirements for immunocompetent co-cultures remain unexplored. To establish suitable conditions for incorporating macrophages into skin models, we evaluated the effects of different media on primary keratinocytes, fibroblasts and macrophages. Skin maturation was affected by culture in macrophage medium, while macrophages showed reduced viability, altered cell morphology and decreased response to pro- and anti-inflammatory stimuli in skin differentiation media, both in 2D and 3D. The results indicate that immunocompetent skin models have specific, complex requirements for supporting an accurate detection of immune responses, which point at the identification of a suitable culture medium as a crucial pre-requisite for the development of physiologically relevant models.
DNA methylation architecture of the ACE2 gene in nasal cells of children
Authors: Cardenas Andres , Rifas-Shiman Sheryl L. , Sordillo Joanne E. , DeMeo Dawn L. , Baccarelli Andrea A. , Hivert Marie-France , Gold Diane R. , Oken Emily
Published: March 2021

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led to the global coronavirus disease 2019 (COVID-19) pandemic. SARS-CoV-2 enters cells via angiotensin-Converting Enzyme 2 (ACE2) receptors, highly expressed in nasal epithelium with parallel high infectivity.1,2 The nasal epigenome is in direct contact with the environment and could explain COVID-19 disparities by reflecting social and environmental influences on ACE2 regulation. We collected nasal swabs from anterior nares of 547 children, measured DNA methylation (DNAm), and tested differences at 15 ACE2 CpGs by sex, age, race/ethnicity and epigenetic age. ACE2 CpGs were differentially methylated by sex with 12 sites having lower DNAm (mean = 12.71%) and 3 sites greater DNAm (mean = 1.45%) among females relative to males. We observed differential DNAm at 5 CpGs for Hispanic females (mean absolute difference = 3.22%) and lower DNAm at 8 CpGs for Black males (mean absolute difference = 1.33%), relative to white participants. Longer DNAm telomere length was associated with greater ACE2 DNAm at 11 and 13 CpGs among males (mean absolute difference = 7.86%) and females (mean absolute difference = 8.21%), respectively. Nasal ACE2 DNAm differences could contribute to our understanding COVID-19 severity and disparities reflecting upstream environmental and social influences. Findings need to be confirmed among adults and patients with risk factors for COVID-19 severity.
Further behavioural parameters support reciprocity and milk theft as explanations for giraffe allonursing
Authors: Gloneková Markéta , Brandlová Karolína , Pluháček Jan
Published: March 2021

Suckling of a non-filial calf, or allosuckling, is an extreme case of allomaternal care in mammals. There have been many hypotheses postulated in an attempt to explain this behaviour; however, the supporting evidence differs, together with the conclusions drawn from the investigated variables. Previously, suckling bout rejection was analysed, and the milk theft and reciprocity hypotheses were both determined as the most appropriate explanation of allosuckling in giraffe. In this study, seven hypotheses were tested using different behavioural parameters, namely suckling bout frequency, suckling bout duration, and time spent suckling. It is well-documented that these parameters are associated with various aspects in ungulate biology; for example, suckling rejection typically reflects milk intake and parent–offspring conflict, whereas the suckling bout duration and frequency is associated with social behaviours (affiliation, bonding, social stress). In total, 22 nursing females and 47 suckling calves were observed, in four Czech zoos during a five-year period. The correlation of the observed parameters between the reciprocal female-calf dyads was found to be in line with the reciprocity hypothesis. In addition, non-filial calves tried to steal the milk from non-maternal females, supporting the milk theft hypothesis. Thus, the results support both the reciprocity and milk-theft hypotheses as the most plausible explanation of allosuckling in giraffe, and illustrates the importance of using suckling bout duration and frequency, and the time spent suckling, as behavioural parameters that may aid in explaining the extremity of maternal investment, such as allosuckling.