bluuebunny/crossref_metadata_2025 huggingface dataset:
Statistical Observations of Proton‐Band Electromagnetic Ion Cyclotron Waves in the Outer Magnetosphere: Full Wavevector Determination
Authors: Toledo‐Redondo S. , Lee J. H. , Vines S. K. , Albert I. F. , André M. , Castilla A. , Dargent J. P. , Fu H. S. , Fuselier S. A. , Genot V. , Graham D. B. , Kitamura N. , Khotyaintsev Yu. V. , Lavraud B. , Montagud‐Camps V. , Navarro E. A. , Norgren C. , Perrone D. , Phan T. D. , Portí J. , Salinas A. , Stawarz J. E. , Vaivads A.
Published: May 2024

Electromagnetic Ion Cyclotron (EMIC) waves mediate energy transfer from the solar wind to the magnetosphere, relativistic electron precipitation, or thermalization of the ring current population, to name a few. How these processes take place depends on the wave properties, such as the wavevector and polarization. However, inferring the wavevector from in‐situ measurements is problematic since one needs to disentangle spatial and time variations. Using 8 years of Magnetospheric Multiscale (MMS) mission observations in the dayside magnetosphere, we present an algorithm to detect proton‐band EMIC waves in the Earth's dayside magnetosphere, and find that they are present roughly 15% of the time. Their normalized frequency presents a dawn‐dusk asymmetry, with waves in the dawn flank magnetosphere having larger frequency than in the dusk, subsolar, and dawn near subsolar region. It is shown that the observations are unstable to the ion cyclotron instability. We obtain the wave polarization and wavevector by comparing Single Value Decomposition and Ampere methods. We observe that for most waves the perpendicular wavenumber (k⊥) is larger than the inverse of the proton gyroradius (ρi), that is, k⊥ρi > 1, while the parallel wavenumber is smaller than the inverse of the ion gyroradius, that is, k‖ρi < 1. Left‐hand polarized waves are associated with small wave normal angles (θBk < 30°), while linearly polarized waves are associated with large wave normal angles (θBk > 30°). This work constitutes, to our knowledge, the first attempt to statistically infer the full wavevector of proton‐band EMIC waves observed in the outer magnetosphere.
African Easterly Wave Strength and Observed Atlantic Tropical Cyclone Genesis and Characteristics
Authors: Bercos‐Hickey Emily , Patricola Christina M.
Published: May 2024

African easterly waves (AEWs) are known precursors to Atlantic tropical cyclones (TCs), and are therefore often directly connected to extreme weather events that can be both deadly and destructive. It is well established that not all AEWs develop into TCs, and there has been substantial research that has addressed the different characteristics and environments of developing and non‐developing waves. In this study, however, we specifically examine 41‐years of developing AEWs to provide a better understanding of the relationship between the developing wave and the environment, and the resulting TC. To conduct this research, we identified TCs with AEW origins from the observational record between 1980 and 2020. We then used an objective tracking algorithm to identify the developing AEWs in reanalysis data. We found a statistically significant relationship between the strength of the developing AEWs, TC genesis location and landfall, and sea surface temperature (SST) during TC genesis. Weaker AEWs tend to develop into TCs closer to the Americas in a region with warmer SSTs than those of the stronger AEWs, which tend to develop into TCs closer to Africa. Consequently, the TCs that develop from weaker AEWs are more likely to make landfall due to the close proximity of their genesis locations to the Americas.
Taurine reduces the risk for metabolic syndrome: a systematic review and meta-analysis of randomized controlled trials
Authors: Tzang Chih-Chen , Chi Liang-Yun , Lin Long-Huei , Lin Ting-Yu , Chang Ke-Vin , Wu Wei-Ting , Özçakar Levent
Published: May 2024

Background

Metabolic syndrome (MetS) is a cluster of interconnected risk factors that significantly increase the likelihood of cardiovascular disease and type 2 diabetes. Taurine has emerged as a potential therapeutic agent for MetS. This meta-analysis of randomized controlled trials (RCTs) aimed to evaluate the effects of taurine supplementation on MetS-related parameters.

Methods

We conducted electronic searches through databases like Embase, PubMed, Web of Science, Cochrane CENTRAL, and ClinicalTrials.gov, encompassing publications up to December 1, 2023. Our analysis focused on established MetS diagnostic criteria, including systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood glucose (FBG), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-C). Meta-regression explored potential dose-dependent relationships based on the total taurine dose administered during the treatment period. We also assessed secondary outcomes like body composition, lipid profile, and glycemic control.

Results

Our analysis included 1024 participants from 25 RCTs. The daily dosage of taurine in the studies ranged from 0.5 g/day to 6 g/day, with follow-up periods varying between 5 and 365 days. Compared to control groups, taurine supplementation demonstrated statistically significant reductions in SBP (weighted mean difference [WMD] = −3.999 mmHg, 95% confidence interval [CI] = −7.293 to −0.706, p = 0.017), DBP (WMD = −1.509 mmHg, 95% CI = −2.479 to −0.539, p = 0.002), FBG (WMD: −5.882 mg/dL, 95% CI: −10.747 to −1.018, p = 0.018), TG (WMD: −18.315 mg/dL, 95% CI: −25.628 to −11.002, p < 0.001), but not in HDL-C (WMD: 0.644 mg/dl, 95% CI: −0.244 to 1.532, p = 0.155). Meta-regression analysis revealed a dose-dependent reduction in DBP (coefficient = −0.0108 mmHg per g, p = 0.0297) and FBG (coefficient = −0.0445 mg/dL per g, p = 0.0273). No significant adverse effects were observed compared to the control group.

Conclusion

Taurine supplementation exhibits positive effects on multiple MetS-related factors, making it a potential dietary addition for individuals at risk of or already experiencing MetS. Future research may explore dose-optimization strategies and potential long-term benefits of taurine for MetS management.
Risk factors for malnutrition in patients with diabetic foot ulcer and its association with prolonged length of hospitalization
Authors: Ran Qian , Xu Weiwei , Zhao Xili , Sun Hang , Liu Li , Luo Yunqiu
Published: May 2024

Purpose

The study was designed to investigate the occurrence and risk factors of malnutrition in diabetic foot ulcers (DFU) patients and examine the association between malnutrition and length of stay (LOS).

Methods

This observational study included DFU hospitalized patients in two campuses of a hospital from January 2021 to June 2023. The diagnosis standard of malnutrition was established by using the Global Leadership Initiative on Malnutrition (GLIM) criteria. Patients were followed up to ascertain the length of hospitalization, and hospital stays longer than 17 days were considered as prolonged LOS. To explore the risk factors of malnutrition and the association between malnutrition and LOS, univariate and multivariate logistic regression analyses were performed.

Results

Overall 219 DFU patients were enrolled, malnutrition was identified in 38.36% of patients according to GLIM criteria, and 92 patients (42%) were recognized as prolonged LOS. Logistic regression analyses showed that BMI (P <0.001), Alb (P = 0.002), HbA1c (P <0.001), ulcer infection (P <0.001), LOS (P = 0.010), and ABI (P = 0.024) were independent risk factors for malnutrition. Besides, malnutrition by GLIM criteria was closely related to prolonged LOS and malnourished DFU patients were 2.857 times (95% CI, 1.497–5.450; P = 0.001) likely to present prolonged LOS than that of normal nutrition.

Conclusion

Malnutrition was considered to be extremely prevalent in DFU patients and was associated with approximately three times higher likelihood of prolonged LOS. Implementing and disseminating the diagnostic criteria during routine practice is crucial, given the predictive efficacy of GLIM criteria.
Differential SNARE chaperoning by Munc13-1 and Munc18-1 dictates fusion pore fate at the release site
Authors: Bhaskar Bhavya R. , Yadav Laxmi , Sriram Malavika , Sanghrajka Kinjal , Gupta Mayank , V Boby K. , Nellikka Rohith K. , Das Debasis
Published: May 2024

The regulated release of chemical messengers is crucial for cell-to-cell communication; abnormalities in which impact coordinated human body function. During vesicular secretion, multiple SNARE complexes assemble at the release site, leading to fusion pore opening. How membrane fusion regulators act on heterogeneous SNARE populations to assemble fusion pores in a timely and synchronized manner, is unknown. Here, we demonstrate the role of SNARE chaperones Munc13-1 and Munc18-1 in rescuing individual nascent fusion pores from their diacylglycerol lipid-mediated inhibitory states. At the onset of membrane fusion, Munc13-1 clusters multiple SNARE complexes at the release site and synchronizes release events, while Munc18-1 stoichiometrically interacts with trans-SNARE complexes to enhance N- to C-terminal zippering. When both Munc proteins are present simultaneously, they differentially access dynamic trans-SNARE complexes to regulate pore properties. Overall, Munc proteins’ direct action on fusion pore assembly indicates their role in controlling quantal size during vesicular secretion.
Extracellular modulation of TREK-2 activity with nanobodies provides insight into the mechanisms of K2P channel regulation
Authors: Rödström Karin E. J. , Cloake Alexander , Sörmann Janina , Baronina Agnese , Smith Kathryn H. M. , Pike Ashley C. W. , Ang Jackie , Proks Peter , Schewe Marcus , Holland-Kaye Ingelise , Bushell Simon R. , Elliott Jenna , Pardon Els , Baukrowitz Thomas , Owens Raymond J. , Newstead Simon , Steyaert Jan , Carpenter Elisabeth P. , Tucker Stephen J.
Published: May 2024

Potassium channels of the Two-Pore Domain (K2P) subfamily, KCNK1-KCNK18, play crucial roles in controlling the electrical activity of many different cell types and represent attractive therapeutic targets. However, the identification of highly selective small molecule drugs against these channels has been challenging due to the high degree of structural and functional conservation that exists not only between K2P channels, but across the whole K+ channel superfamily. To address the issue of selectivity, here we generate camelid antibody fragments (nanobodies) against the TREK-2 (KCNK10) K2P K+ channel and identify selective binders including several that directly modulate channel activity. X-ray crystallography and CryoEM data of these nanobodies in complex with TREK-2 also reveal insights into their mechanisms of activation and inhibition via binding to the extracellular loops and Cap domain, as well as their suitability for immunodetection. These structures facilitate design of a biparatropic inhibitory nanobody with markedly improved sensitivity. Together, these results provide important insights into TREK channel gating and provide an alternative, more selective approach to modulation of K2P channel activity via their extracellular domains.
A conceptual analysis of gendered energy care work and epistemic injustice through a case study of Zanzibar’s Solar Mamas
Authors: Michael Kavya , Ahlborg Helene
Published: May 2024

Energy and climate transitions bear an inherent risk of replicating historically embedded unjust gendered norms in the current energy regimes. Positioning our work within critical feminist scholarship, our study emphasizes the embedded nature of energy technologies within respective socio-economic, institutional and cultural contexts. We use a combined lens of care and epistemic injustice to examine the case study of Solar Mamas in Barefoot College Zanzibar, highlighting the nuanced interplay of power relations in decentralized energy transitions. This approach helps comprehend and value gendered energy care work as involving skilled labour in everyday life. Our findings illustrate the need for energy transitions research, policy and practice to be deeply informed by lived experiences, diverse practices of care within the energy webs and valuing of multiple voices. We argue that interventions prioritizing care and knowledge in decentralized, locally managed energy provisioning have the potential to disrupt established gender relations.
A novel skin cancer detection model using modified finch deep CNN classifier model
Authors: Kumar Ashwani , Kumar Mohit , Bhardwaj Ved Prakash , Kumar Sunil , Selvarajan Shitharth
Published: May 2024

Skin cancer is one of the most life-threatening diseases caused by the abnormal growth of the skin cells, when exposed to ultraviolet radiation. Early detection seems to be more crucial for reducing aberrant cell proliferation because the mortality rate is rapidly rising. Although multiple researches are available based on the skin cancer detection, there still exists challenges in improving the accuracy, reducing the computational time and so on. In this research, a novel skin cancer detection is performed using a modified falcon finch deep Convolutional neural network classifier (Modified Falcon finch deep CNN) that efficiently detects the disease with higher efficiency. The usage of modified falcon finch deep CNN classifier effectively analyzed the information relevant to the skin cancer and the errors are also minimized. The inclusion of the falcon finch optimization in the deep CNN classifier is necessary for efficient parameter tuning. This tuning enhanced the robustness and boosted the convergence of the classifier that detects the skin cancer in less stipulated time. The modified falcon finch deep CNN classifier achieved accuracy, sensitivity, and specificity values of 93.59%, 92.14%, and 95.22% regarding k-fold and 96.52%, 96.69%, and 96.54% regarding training percentage, proving more effective than literary works.
Preoperatively predicting the lymph node metastasis and prognosis for gastric cancer patients
Authors: Wang Danfang , Wang Yaxin , Dong Lin , Zhang Xin , Du Jianfei
Published: May 2024

The preoperative distinguishment of lymph nodes (LN) with metastasis plays a pivotal role in guiding the surgical extension for gastric cancer (GC). We aim to identify the preparative risk factors for LN metastasis in GC patients. We retrospectively reviewed 424 patients who underwent radical GC resection in our medical center between Jan 2011 and Dec 2018. Multivariate logistic regression was employed to identify risk factors for LN metastasis, while multivariate COX regression was utilized to evaluate prognostic factors. The median overall survival of patients with or without LN metastases was 31 and 58 months, respectively. In multivariate analysis, lower albumin (OR = 0.512; P = 0.004) and prealbumin (OR = 0.367, P = 0.001) and higher CEA (OR = 3.178, P < 0.001), CA199 (OR = 2.278, P = 0.002) and platelets (OR = 1.697, P = 0.017) were found to be significantly associated with LN metastasis. In survival analysis, older age (HR = 1.712), larger tumors (HR = 1.082), higher D-dimer (HR = 1.561) and CA199 (HR = 1.553), advanced staging (stage II, HR = 3.446; stage III-IV, HR = 11.089), lower prealbumin levels (lower level for reference, HR = 0.63), and absence of adjuvant chemotherapy (HR = 0.396) was discovered to be associated with poorer overall survival (all P < 0.05). In conclusion, our results demonstrated that preoperative prealbumin-bound tumor markers can effectively predict LN metastasis. Additionally, prealbumin was found to possess prognostic value as well.
Revealing the pharmacological mechanisms of nao-an dropping pill in preventing and treating ischemic stroke via the PI3K/Akt/eNOS and Nrf2/HO-1 pathways
Authors: Wang Chen , Xiong Zhe-Ming , Cong You-Quan , Li Zi-Yao , Xie Yi , Wang Ying-Xiao , Zhou Hui-Min , Yang Yan-Fang , Liu Jing-Jing , Wu He-Zhen
Published: May 2024

Nao-an Dropping Pill (NADP) is a Chinese patent medicine which commonly used in clinic for ischemic stroke (IS). However, the material basis and mechanism of its prevention or treatment of IS are unclear, then we carried out this study. 52 incoming blood components were resolved by UHPLC-MS/MS from rat serum, including 45 prototype components. The potential active prototype components hydroxysafflor yellow A, ginsenoside F1, quercetin, ferulic acid and caffeic acid screened by network pharmacology showed strongly binding ability with PIK3CA, AKT1, NOS3, NFE2L2 and HMOX1 by molecular docking. In vitro oxygen–glucose deprivation/reperfusion (OGD/R) experimental results showed that NADP protected HA1800 cells from OGD/R-induced apoptosis by affecting the release of LDH, production of NO, and content of SOD and MDA. Meanwhile, NADP could improve behavioral of middle cerebral artery occlusion/reperfusion (MCAO/R) rats, reduce ischemic area of cerebral cortex, decrease brain water and glutamate (Glu) content, and improve oxidative stress response. Immunohistochemical results showed that NADP significantly regulated the expression of PI3K, Akt, p-Akt, eNOS, p-eNOS, Nrf2 and HO-1 in cerebral ischemic tissues. The results suggested that NADP protects brain tissues and ameliorates oxidative stress damage to brain tissues from IS by regulating PI3K/Akt/eNOS and Nrf2/HO-1 signaling pathways.
Patient classification and attribute assessment based on machine learning techniques in the qualification process for surgical treatment of adrenal tumours
Authors: Wielogórska-Partyka Marta , Adamski Marcin , Siewko Katarzyna , Popławska-Kita Anna , Buczyńska Angelika , Myśliwiec Piotr , Krętowski Adam Jacek , Adamska Agnieszka
Published: May 2024

Adrenal gland incidentaloma is frequently identified through computed tomography and poses a common clinical challenge. Only selected cases require surgical intervention. The primary aim of this study was to compare the effectiveness of selected machine learning (ML) techniques in proper qualifying patients for adrenalectomy and to identify the most accurate algorithm, providing a valuable tool for doctors to simplify their therapeutic decisions. The secondary aim was to assess the significance of attributes for classification accuracy. In total, clinical data were collected from 33 patients who underwent adrenalectomy. Histopathological assessments confirmed the proper selection of 21 patients for surgical intervention according to the guidelines, with accuracy reaching 64%. Statistical analysis showed that Supported Vector Machines (linear) were significantly better than the baseline (p < 0.05), with accuracy reaching 91%, and imaging features of the tumour were found to be the most crucial attributes. In summarise, ML methods may be helpful in qualifying patients for adrenalectomy.
Prevalence and patterns of vitamin D deficiency and its role in cognitive functioning in a cohort from South India
Authors: Ghosh Aishwarya , S Monisha , Sunny Albert Stezin , Diwakar Latha , Issac Thomas Gregor
Published: May 2024

Vitamin D (VitD) is a naturally occurring, fat-soluble vitamin which regulates calcium and phosphate homeostasis in the human body and is also known to have a neuroprotective role. VitD deficiency has often been associated with impaired cognition and a higher risk of dementia. In this study, we aimed to explore the relationship between levels of VitD and cognitive functioning in adult individuals. 982 cognitively healthy adults (≥ 45 years) were recruited as part of the CBR-Tata Longitudinal Study for Aging (TLSA). Addenbrooke’s cognitive examination-III (ACE-III) and Hindi mental status examination (HMSE) were used to measure cognitive functioning. 25-hydroxyvitamin D [25(OH)D] levels were measured from the collected serum sample and classified into three groups— deficient (< 20 ng/ml), insufficient (20–29 ng/ml) and normal (≥ 30 ng/ml). Statistical analysis was done using IBM SPSS software, version 28.0.1.1(15). The mean age of the participants was 61.24 ± 9 years. Among 982 participants, 572 (58%) were deficient, 224 (23%) insufficient and only 186 (19%) had normal levels of VitD. Kruskal–Wallis H test revealed a significant difference in age (p = 0.015) and education (p = 0.021) across VitD levels and the Chi-square test revealed a significant association between gender (p = 0.001) and dyslipidemia status (p = 0.045) with VitD levels. After adjusting for age, education, gender and dyslipidemia status, GLM revealed that individuals with deficient (p = 0.038) levels of VitD had lower scores in ACE-III verbal fluency as compared to normal. Additionally, we also found that 91.2% individuals who had VitD deficiency were also having dyslipidemia. It is concerning that VitD deficiency impacts lipid metabolism. Lower levels of VitD also negatively impacts verbal fluency in adult individuals. Verbal fluency involves higher order cognitive functions and this result provides us with a scope to further investigate the different domains of cognition in relation to VitD deficiency and other associated disorders.
Seasonal fish larvae abundance and composition in seagrass habitats of coastal East Africa
Authors: Ngisiange Noah , Tarimo Barnabas , Daudi Lillian , Mwangi Stephen , Malesa Fadhili , George Rushingisha , Kyewalyanga Margareth S. , Gullström Martin , Osore Melckzedeck , Mwaluma James , Winder Monika
Published: May 2024

Seagrass habitats play a major role in fisheries productivity through nursery functions and feeding grounds for diverse fish species. However, little is known about the seasonal distribution of fish larvae at large spatial scales in coastal East Africa. We investigated drivers of the seasonal fish larvae abundance and composition in seagrass habitats in Kenya and Tanzania. We found a high diversity of fish larvae (54 families) inhabiting seagrass habitats that differed between sites and seasons. Fish larvae abundance were highest in Kenya, particularly during the northeast monsoon season. Overall, total larval abundances per site were low, reaching less than 190 individuals/100 m3 in Kenya and less than 40 individuals/100 m3 in Tanzania, likely related to the low productivity and strong hydrodynamic processes in this region. Our data suggests that most of the fish spawn year-round in these tropical waters as we did not find strong seasonal patterns. All sites had a high relative abundance of larvae from demersal spawning fishes, indicating that many fish species move to coastal sites for spawning. Primary productivity and dissolved oxygen, driven by hydrodynamics conditions are positively related to fish larvae productivity both in Kenya and Tanzania. These findings indicate that the occurrence of both resident and transient fish larvae in seagrass meadows is driven by strong hydrodynamic and tidal processes that transport fish larvae across adjacent habitats.
Effects of nordic walking training on gait and exercise tolerance in male ischemic heart disease patients
Authors: Szpala Agnieszka , Winiarski Sławomir , Kołodziej Małgorzata , Jasiński Ryszard , Lejczak Andrzej , Kałka Dariusz , Lorek Karolina , Bałchanowski Jacek , Wudarczyk Sławomir , Woźniewski Marek , Pietraszewski Bogdan
Published: May 2024

This technique-focused observational study explores the impact of a 6-week Nordic Walking (NW) program on physiological and biomechanical aspects in ischemic heart disease (IHD) patients. Twelve male IHD patients (66.2 ± 5.2 years, 12.2 ± 7.5 years of disease duration) were evaluated pre- and post-training for (i) gait parameters, (ii) exercise tolerance using electrocardiographic (ECG) stress test, (iii) a 6-min walk test (6MWT). The NW training, adhering to IHD patient guidelines, involved a 100-m walk at a self-selected, preferred speed without sticks, with classic NW sticks and mechatronic sticks. A mechatronic measuring system, specifically engineered for measuring, diagnosing and monitoring the patient's gait, was integrated into mechatronic sticks. Post-training, significant enhancements were observed in ECG stress test duration, metabolic equivalency, and 6MWT distance, irrespective of the stick type. However, no significant changes were noted in spatiotemporal parameters concerning the measured side, stick utilisation, or type. The results suggest that NW training boosts exercise capacity and refines gait mechanics in male IHD patients. However, the improvement in exercise capacity was not linked to changes in gait mechanics from NW training but rather to the movement during NW gait. Hence, the key to enhancing exercise capacity in IHD patients is the movement during NW gait, not the quality of gait mechanics.
Cryptosporidium spp. in large-scale sheep farms in China: prevalence and genetic diversity
Authors: Zhao Qianming , Qi Meng , Jing Bo , Jian Fuchun , Gong Pihong , Lu Chenyang , Yan Yaqun , Pei Zhiyang , Ning Changshen
Published: May 2024

Cryptosporidium spp. are significant zoonotic intestinal parasites that induce diarrhea and even death across most vertebrates, including humans. Previous studies showed that sheep are important hosts for Cryptosporidium and that its distribution in sheep is influenced by geography, feeding patterns, age, and season. Environmental factors also influence the transmission of Cryptosporidium. Molecular studies of Cryptosporidium in sheep have been conducted in only a few regions of China, and studies into the effect of sheep-housing environments on Cryptosporidium transmission are even rarer. To detect the prevalence of Cryptosporidium in large-scale sheep-housing farms, a total of 1241 fecal samples were collected from sheep, 727 environmental samples were taken from sheep housing, and 30 water samples were collected in six regions of China. To ascertain the existence of the parasite and identify the species of Cryptosporidium spp., we conducted nested PCR amplification of DNA extracted from all samples using the small-subunit (SSU) rRNA gene as a target. For a more in-depth analysis of Cryptosporidium spp. subtypes, C. xiaoi-and C. ubiquitum-positive samples underwent separate nested PCR amplification targeting the 60 kDa glycoprotein (gp60) gene. The amplification of the Cryptosporidium spp. SSU rRNA gene locus from the whole genomic DNA of all samples yielded a positive rate of 1.2% (20/1241) in fecal samples, 0.1% (1/727) in environmental samples, and no positive samples were found in water samples. The prevalence of Cryptosporidium spp. infection in large-scale housed sheep was 1.7%, which was higher than that in free-ranging sheep (0.0%). The highest prevalence of infection was found in weaning lambs (6.8%). Among the different seasons, the peaks were found in the fall and winter. The most prevalent species were C. xiaoi and C. ubiquitum, with the former accounting for the majority of infections. The distribution of C. xiaoi subtypes was diverse, with XXIIIc (n = 1), XXIIId (n = 2), XXIIIe (n = 2), and XXIIIl (n = 4) identified. In contrast, only one subtype, XIIa (n = 9), was found in C. ubiquitum. In this study, C. xiaoi and C. ubiquitum were found to be the predominant species, and Cryptosporidium was found to be present in the environment. These findings provide an important foundation for the comprehensive prevention and management of Cryptosporidium in intensively reared sheep. Furthermore, by elucidating the prevalence of Cryptosporidium in sheep and its potential role in environmental transmission, this study deepens our understanding of the intricate interactions between animal health, environmental contamination, and public health dynamics.
Unsupervised detection of large-scale weather patterns in the northern hemisphere via Markov State Modelling: from blockings to teleconnections
Authors: Springer Sebastian , Laio Alessandro , Galfi Vera Melinda , Lucarini Valerio
Published: May 2024

Detecting recurrent weather patterns and understanding the transitions between such regimes are key to advancing our knowledge of the low-frequency variability of the atmosphere and have important implications in terms of weather and climate-related risks. We adopt an analysis pipeline inspired by Markov State Modelling and detect in an unsupervised manner the dominant winter mid-latitude Northern Hemisphere weather patterns in the Atlantic and Pacific sectors. The daily 500 hPa geopotential height fields are first classified in about 200 microstates. The weather dynamics are then represented on the basis of these microstates and the slowest decaying modes are identified from the spectral properties of the transition probability matrix. These modes are defined on the basis of the nonlinear dynamical processes of the system and not as tentative metastable states, as often done in Markov state analysis. When focusing on a shifting longitudinal window of 60∘, we find that the longitude-dependent estimate of the longest relaxation time is smaller where stronger baroclinic activity is found. In the Atlantic and Pacific sectors slow relaxation processes are mainly related to transitions between blocked regimes and zonal flow. We also find strong evidence of a dynamical regime associated with the simultaneous Atlantic-Pacific blocking. When the analysis is performed on a broader geographical region of the Atlantic sector, we discover that the slowest relaxation modes of the system are associated with transitions between dynamical regimes that resemble teleconnection patterns like the North Atlantic Oscillation and weather regimes like the Scandinavian and Greenland blocking, yet have a much stronger dynamical foundation than classical methods based e.g. on EOF analysis. Our method clarifies that, as a result of the lack of a time-scale separation in the atmospheric variability of the mid-latitudes, there is no clear-cut way to represent the atmospheric dynamics in terms of few, well-defined modes of variability. The approach proposed here can be seamlessly applied across different regions of the globe for detecting regional modes of variability, and has a great potential for intercomparing climate models and for assessing the impact of climate change on the low-frequency variability of the atmosphere.
Competing impacts of tropical Pacific and Atlantic on Southern Ocean inter-decadal variability
Authors: Yao Shuai-Lei , Wu Renguang , Luo Jing-Jia , Zhou Wen
Published: May 2024

The observed Southern Ocean sea surface temperature (SST) has experienced prominent inter-decadal variability nearly in phase with the Inter-decadal Pacific Oscillation (IPO), but less associated with the Atlantic Multidecadal Variability (AMV), challenging the prevailing view of Pacific-Atlantic synergistic effects. Yet, the mechanisms of distinct trans-hemispheric connections to the Southern Ocean remain indecisive. Here, by individually constraining the observed cold-polarity and warm-polarity IPO and AMV SSTs in a climate model, we show that the IPO is influential in initiating a basin-wide Southern Ocean response, with the AMV secondary. A tropical Pacific-wide cooling triggers a basin-scale Southern Ocean cold episode through a strong Rossby wave response to the north-to-south cross-equatorial weakened Hadley circulation. By contrast, due to the competing role of tropical Pacific cooling, an Atlantic warming partly cools the Southern Ocean via a weak Rossby wave response to the south-to-north cross-equatorial enhanced Hadley circulation. Conversely, tropical Pacific warming leads to a warm Southern Ocean episode. Our findings highlight that properly accounting for the tropical Pacific SST variability may provide a potential for skillful prediction of Southern Ocean climate change and more reliable estimates of climate sensitivity, currently overestimated by the misrepresented Southern Ocean warming.
Immunotherapy for recurrent or metastatic nasopharyngeal carcinoma
Authors: Liu Xin , Shen Hui , Zhang Lu , Huang Wenhui , Zhang Shuixing , Zhang Bin
Published: May 2024

Immunotherapy, particularly immune checkpoint inhibitors (ICIs), such as anti-programmed death 1/programmed death-ligand 1 (PD-1/PD-L1) therapy, has emerged as a pivotal treatment modality for solid tumors, including recurrent or metastatic nasopharyngeal carcinoma (R/M-NPC). Despite the advancements in the utilization of ICIs, there is still room for further improving patient outcomes. Another promising approach to immunotherapy for R/M-NPC involves adoptive cell therapy (ACT), which aims to stimulate systemic anti-tumor immunity. However, individual agent therapies targeting dendritic cells (DCs) appear to still be in the clinical trial phase. This current review underscores the potential of immunotherapy as a valuable adjunct to the treatment paradigm for R/M-NPC patients. Further research is warranted to enhance the efficacy of immunotherapy through the implementation of strategies such as combination therapies and overcoming immune suppression. Additionally, the development of a biomarker-based scoring system is essential for identifying suitable candidates for precision immunotherapy.
M2 macrophage-derived exosomal circTMCO3 acts through miR-515-5p and ITGA8 to enhance malignancy in ovarian cancer
Authors: Ran Xiao-Min , Yang Juan , Wang Zi-Yi , Xiao Ling-Zhi , Deng Yu-Ping , Zhang Ke-Qiang
Published: May 2024

Tumor-associated macrophages of the M2 phenotype promote cancer initiation and progression. Importantly, M2 macrophage-derived exosomes play key roles in the malignancy of cancer cells. Here, we report that circTMCO3 is upregulated in ovarian cancer patients, and its high expression indicates poor survival. M2-derived exosomes promote proliferation, migration, and invasion in ovarian cancer, but these effects are abolished by knockdown of circTMCO3. Furthermore, circTMCO3 functions as a competing endogenous RNA for miR-515-5p to reduce its abundance, thus upregulating ITGA8 in ovarian cancer. miR-515-5p inhibits ovarian cancer malignancy via directly downregulating ITGA8. The decreased oncogenic activity of circTMCO3-silencing exosomes is reversed by miR-515-5p knockdown or ITGA8 overexpression. Exosomal circTMCO3 promotes ovarian cancer progression in nude mice. Thus, M2 macrophage-derived exosomes promote malignancy by delivering circTMCO3 and targeting the miR-515-5p/ITGA8 axis in ovarian cancer. Our findings not only provide mechanistic insights into ovarian cancer progression, but also suggest potential therapeutic targets.
Taking a shortcut: what mechanisms do fish use?
Authors: Sibeaux Adelaide , Newport Cait , Green Jonathan P. , Karlsson Cecilia , Engelmann Jacob , Burt de Perera Theresa
Published: May 2024

Path integration is a powerful navigational mechanism whereby individuals continuously update their distance and angular vector of movement to calculate their position in relation to their departure location, allowing them to return along the most direct route even across unfamiliar terrain. While path integration has been investigated in several terrestrial animals, it has never been demonstrated in aquatic vertebrates, where movement occurs through volumetric space and sensory cues available for navigation are likely to differ substantially from those in terrestrial environments. By performing displacement experiments with Lamprologus ocellatus, we show evidence consistent with fish using path integration to navigate alongside other mechanisms (allothetic place cues and route recapitulation). These results indicate that the use of path integration is likely to be deeply rooted within the vertebrate phylogeny irrespective of the environment, and suggests that fish may possess a spatial encoding system that parallels that of mammals.
Mercury contamination is an invisible threat to declining migratory shorebirds along the East Asian-Australasian Flyway
Authors: Ma Yanju , Choi Chi-Yeung , Shang Lihai , Klaassen Marcel , Ma Zhijun , Chang Qing , Jaspers Veerle L. B. , Bai Qingquan , He Tao , Leung Katherine K-S. , Hassell Chris J. , Jessop Roz , Gibson Luke
Published: May 2024

Exposure to pollutants is a potentially crucial but overlooked driver of population declines in shorebirds along the East Asian-Australasian Flyway. We combined knowledge of moult strategy and life history with a standardised sampling protocol to assess mercury (Hg) contamination in 984 individuals across 33 migratory shorebird species on an intercontinental scale. Over one-third of the samples exceeded toxicity benchmarks. Feather Hg was best explained by moulting region, while habitat preference (coastal obligate vs. non-coastal obligate), the proportion of invertebrates in the diet and foraging stratum (foraging mostly on the surface vs. at depth) also contributed, but were less pronounced. Feather Hg was substantially higher in South China (Mai Po and Leizhou), Australia and the Yellow Sea than in temperate and Arctic breeding ranges. Non-coastal obligate species (Tringa genus) frequently encountered in freshwater habitats were at the highest risk. It is important to continue and expand biomonitoring research to assess how other pollutants might impact shorebirds.
The neurocomputational link between defensive cardiac states and approach-avoidance arbitration under threat
Authors: Klaassen Felix H. , de Voogd Lycia D. , Hulsman Anneloes M. , O’Reilly Jill X. , Klumpers Floris , Figner Bernd , Roelofs Karin
Published: May 2024

Avoidance, a hallmark of anxiety-related psychopathology, often comes at a cost; avoiding threat may forgo the possibility of a reward. Theories predict that optimal approach-avoidance arbitration depends on threat-induced psychophysiological states, like freezing-related bradycardia. Here we used model-based fMRI analyses to investigate whether and how bradycardia states are linked to the neurocomputational underpinnings of approach-avoidance arbitration under varying reward and threat magnitudes. We show that bradycardia states are associated with increased threat-induced avoidance and more pronounced reward-threat value comparison (i.e., a stronger tendency to approach vs. avoid when expected reward outweighs threat). An amygdala-striatal-prefrontal circuit supports approach-avoidance arbitration under threat, with specific involvement of the amygdala and dorsal anterior cingulate (dACC) in integrating reward-threat value and bradycardia states. These findings highlight the role of human freezing states in value-based decision making, relevant for optimal threat coping. They point to a specific role for amygdala/dACC in state-value integration under threat.
Circadian and photoperiodic regulation of the vegetative to reproductive transition in plants
Authors: Wang Fang , Han Tongwen , Jeffrey Chen Z.
Published: May 2024

As sessile organisms, plants must respond constantly to ever-changing environments to complete their life cycle; this includes the transition from vegetative growth to reproductive development. This process is mediated by photoperiodic response to sensing the length of night or day through circadian regulation of light-signaling molecules, such as phytochromes, to measure the length of night to initiate flowering. Flowering time is the most important trait to optimize crop performance in adaptive regions. In this review, we focus on interplays between circadian and light signaling pathways that allow plants to optimize timing for flowering and seed production in Arabidopsis, rice, soybean, and cotton. Many crops are polyploids and domesticated under natural selection and breeding. In response to adaptation and polyploidization, circadian and flowering pathway genes are epigenetically reprogrammed. Understanding the genetic and epigenetic bases for photoperiodic flowering will help improve crop yield and resilience in response to climate change.
Amino acid insertion in Bat MHC-I enhances complex stability and augments peptide presentation
Authors: Wang Suqiu , Zheng Liangzhen , Wei Xiaohui , Qu Zehui , Du Liubao , Wang Sheng , Zhang Nianzhi
Published: May 2024

Bats serve as reservoirs for numerous zoonotic viruses, yet they typically remain asymptomatic owing to their unique immune system. Of particular significance is the MHC-I in bats, which plays crucial role in anti-viral response and exhibits polymorphic amino acid (AA) insertions. This study demonstrated that both 5AA and 3AA insertions enhance the thermal stability of the bat MHC-I complex and enrich the diversity of bound peptides in terms of quantity and length distribution, by stabilizing the 310 helix, a region prone to conformational changes during peptide loading. However, the mismatched insertion could diminish the stability of bat pMHC-I. We proposed that a suitable insertion may help bat MHC-I adapt to high body temperatures during flight while enhancing antiviral responses. Moreover, this site-specific insertions may represent a strategy of evolutionary adaptation of MHC-I molecules to fluctuations in body temperature, as similar insertions have been found in other lower vertebrates.
Improved detection of magnetic interactions in proteins based on long-lived coherences
Authors: Ianc Octavian , Teleanu Florin , Ciumeică Andrei , Lupulescu Adonis , Sadet Aude , Vasos Paul R.
Published: May 2024

Living systems rely on molecular building blocks with low structural symmetry. Therefore, constituent amino acids and nucleotides yield short-lived nuclear magnetic responses to electromagnetic radiation. Magnetic signals are at the basis of molecular imaging, structure determination and interaction studies. In solution state, as the molecular weight of analytes increases, coherences with long lifetimes are needed to yield advantageous through-space magnetisation transfers. Interactions between magnetic nuclei can only be detected provided the lifetimes of spin order are sufficient. In J-coupled pairs of nuclei, long-lived coherences (LLC’s) connect states with different spin-permutation symmetry. Here in, we show sustained LLC’s in protein Lysozyme, weighing 14.3 kDa, with lifetimes twice as long as those of classical magnetisation for the aliphatic protons of glycine residues. We found for the first time that, in a protein of significant molecular weight, LLC’s yield substantial through-space magnetisation transfers: spin-order transfer stemming from LLC’s overcame transfers from classical coherences by factors > 2. Furthermore, in agreement with theory, the permutation symmetry of LLC-based transfers allows mapping interacting atoms in the protein structure with respect to the molecular plane of glycine residues in a stereospecific manner. These findings can extend the scope of liquid-state high-resolution biomolecular spectroscopy.
A machine learning estimator trained on synthetic data for real-time earthquake ground-shaking predictions in Southern California
Authors: Monterrubio-Velasco Marisol , Callaghan Scott , Modesto David , Carrasco Jose Carlos , Badia Rosa M. , Pallares Pablo , Vázquez-Novoa Fernando , Quintana-Ortí Enrique S. , Pienkowska Marta , de la Puente Josep
Published: May 2024

After large-magnitude earthquakes, a crucial task for impact assessment is to rapidly and accurately estimate the ground shaking in the affected region. To satisfy real-time constraints, intensity measures are traditionally evaluated with empirical Ground Motion Models that can drastically limit the accuracy of the estimated values. As an alternative, here we present Machine Learning strategies trained on physics-based simulations that require similar evaluation times. We trained and validated the proposed Machine Learning-based Estimator for ground shaking maps with one of the largest existing datasets (<100M simulated seismograms) from CyberShake developed by the Southern California Earthquake Center covering the Los Angeles basin. For a well-tailored synthetic database, our predictions outperform empirical Ground Motion Models provided that the events considered are compatible with the training data. Using the proposed strategy we show significant error reductions not only for synthetic, but also for five real historical earthquakes, relative to empirical Ground Motion Models.
Molecular analysis for ovarian cancer detection in patient-friendly samples
Authors: Wever Birgit M. M. , Schaafsma Mirte , Bleeker Maaike C. G. , van den Burgt Yara , van den Helder Rianne , Lok Christianne A. R. , Dijk Frederike , van der Pol Ymke , Mouliere Florent , Moldovan Norbert , van Trommel Nienke E. , Steenbergen Renske D. M.
Published: May 2024

Background

High ovarian cancer mortality rates motivate the development of effective and patient-friendly diagnostics. Here, we explored the potential of molecular testing in patient-friendly samples for ovarian cancer detection.

Methods

Home-collected urine, cervicovaginal self-samples, and clinician-taken cervical scrapes were prospectively collected from 54 patients diagnosed with a highly suspicious ovarian mass (benign n = 25, malignant n = 29). All samples were tested for nine methylation markers, using quantitative methylation-specific PCRs that were verified on ovarian tissue samples, and compared to non-paired patient-friendly samples of 110 age-matched healthy controls. Copy number analysis was performed on a subset of urine samples of ovarian cancer patients by shallow whole-genome sequencing.

Results

Three methylation markers are significantly elevated in full void urine of ovarian cancer patients as compared to healthy controls (C2CD4D, P = 0.008; CDO1, P = 0.022; MAL, P = 0.008), of which two are also discriminatory in cervical scrapes (C2CD4D, P = 0.001; CDO1, P = 0.004). When comparing benign and malignant ovarian masses, GHSR shows significantly elevated methylation levels in the urine sediment of ovarian cancer patients (P = 0.024). Other methylation markers demonstrate comparably high methylation levels in benign and malignant ovarian masses. Cervicovaginal self-samples show no elevated methylation levels in patients with ovarian masses as compared to healthy controls. Copy number changes are identified in 4 out of 23 urine samples of ovarian cancer patients.

Conclusions

Our study reveals increased methylation levels of ovarian cancer-associated genes and copy number aberrations in the urine of ovarian cancer patients. Our findings support continued research into urine biomarkers for ovarian cancer detection and highlight the importance of including benign ovarian masses in future studies to develop a clinically useful test.
Zero-sum beliefs and the avoidance of political conversations
Authors: Boland F. Katelynn , Davidai Shai
Published: May 2024

Although researchers have argued that exposure to diverse views may help reduce political divisions in society, people often avoid discussing politics with ideologically opposed others. We investigate the avoidance of political conversations surrounding highly contested elections in Israel and the U.S. Specifically, we examine the relationship between people’s belief that politics is a zero-sum game and their tendency to avoid talking about politics with ideologically opposed others. In two studies conducted in the days leading up to their countries’ elections, we found that Israeli and American voters who view politics as zero-sum avoided political discussions with ideologically opposed others. Furthermore, zero-sum beliefs about politics statistically predicted the avoidance of political conversations through two distinct mechanisms: perceived conflict and a lack of receptiveness to opposing views. Finally, in a longitudinal design, we found that zero-sum beliefs about politics statistically and robustly predicted the avoidance of political conversation one week later.
Utilization of Musa acuminata blossom peel waste mediated heterogeneous catalyst for biodiesel production from sesame oil
Authors: Sarkar Manoranjan , Daimari Jennifer , Basumatary Sunshri , Kumar Kushwaha Jashvant , Das Ranjay , Deka Anamika Kalita
Published: May 2024

The peel waste of the Musa acuminata blossom after fruit maturation was used for the preparation of a heterogeneous catalyst.
UV-assisted photochemical transformation of a tetranuclear copper(ii) complex: a DFT supported study on β-lactamase inhibitory activity towards antibiotic resistance
Authors: Biswas Sneha , Karim Suhana , Bhunia Pradip , Banerjee Soumadip , Das Abhijit K. , Das Debasis
Published: May 2024

Cu(ii) complex 1, initially inefficient in nitrocefin hydrolysis, becomes a UV-responsive photocatalyst (complex 2) and efficiently hydrolyzes nitrocefin in a methanol–water medium, facilitated by bridged hydroxide.