bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Abscesses in children caused by invasive group A Streptococcus
Authors: Guo Danchun , Zhuang Shuting , Lu Qinghua , Chen Yunsheng , Meng Qing , Sun Lifang , Zheng Yuejie , Wang Wenjian , Yu Dingle
Published: August 2024

Streptococcus is one of the common pathogens of suppurative infections. Invasive group A Streptococcus (iGAS) infections often develop from skin or soft tissue infections, and streptococcal toxic shock syndrome is considered the main cause of death in Chinese children with iGAS infectious disease. However, soft tissue infections caused by iGAS infections, especially the formation of abscesses, are relatively rare. A retrospective study was conducted, and pediatric in-patients who were diagnosed with an iGAS infection identified by cultures from normally sterile sites and treated in a tertiary hospital during 2016–2018 were included. A total of 14 patients were identified, which included 10 boys and four girls. The patients had an age range from 3 months to 10 years and were diagnosed with soft tissue infections and a formation of abscesses caused by iGAS infections. The most common sites of infections were the lower limbs. In five patients, the abscess was accompanied by fever, and the local soft tissue showed redness, swelling, tenderness, and an elevated skin temperature. Laboratory findings included an increased white blood cell (WBC) count in 12 patients, an increased C reactive protein (CRP) level in seven patients, and an increased erythrocyte sedimentation rate (ESR) in 10 patients. No patients had an elevated procalcitonin level. For all 14 patients, we performed puncture and drainage of abscesses, and cultured GAS from the drainage fluid. All children also received antibiotic treatment. During 2 months of follow-up, the patients' condition remained stable and no evidence of kidney or heart damage was observed. For pediatric patients with abscesses, early diagnosis, prompt treatment with incision and drainage, and immediate culture of the drainage fluid are important. Upon confirmation of an iGAS infection, β-lactam antibiotics should be given to provide effective treatment, and in some patients with poor therapeutic outcomes, the use of vancomycin as an alternative can achieve the desired results.
Structural modifications and kinetic effects of KRAS interactions with HRAS and NRAS: an in silico comparative analysis of KRAS mutants
Authors: Silverman Isaac , Gerber Michael , Shaykevich Aaron , Stein Yitzchak , Siegman Alexander , Goel Sanjay , Maitra Radhashree
Published: August 2024

The RAS genes which code for KRAS, HRAS, and NRAS are three of the most frequently mutated oncogenes responsible for cancer deaths. Tumorigenesis is one of the most significant outcomes of deregulation of RAS GTPases. Although the structures have been extensively studied, there is still more to be discovered about the actual binding conformations of the three isoforms, especially when mutated, to design an inhibitory drug. Recent studies have identified important interactions between the three isoforms that affect the oncogenic strength of the others when they are mutated. In this study, we utilize molecular dynamics simulations to examine the modifications of the structural property, mechanism, and kinetic energy of KRAS when interacting individually and with HRAS and NRAS. Notably, we found that WT-KRAS’ orientation when bound to WT-HRAS vs. WT-NRAS is rotated 180°, with mutants demonstrating a similar binding pattern. The binding sites of the isoforms with KRAS share similarities with those involved in the GDP/GTP active site and site of KRAS dimerization. Thus, the isoform interaction can serve as an inhibitory method of KRAS actions. This study advances the understanding of inhibiting RAS-driven cancers through a novel isoform interaction approach only recently discovered, which has been proven to be an effective alternate therapeutic approach. We developed a blueprint of the interaction which would be beneficial in the development of KRAS mutant-specific and pan-KRAS mutant inhibitory drugs that mimic the isoform interactions. Our results support the direct interaction inhibition mechanism of mutant KRAS when bound to WT-HRAS and WT-NRAS by the isoforms’ hypervariable region binding to the G-domain of KRAS. Furthermore, our results support the approach of reducing the effects of oncogenic KRAS by altering the concentration of the isoforms or a drug alternative based on the overall structural and kinetic stability, as well as the binding strength of the mutant-isoform complexes.
Frequency-specific static and dynamic neural activity indices in children with different attention deficit hyperactivity disorder subtypes: a resting-state fMRI study
Authors: Chen Ran , Jiao Yun , Zhu Jun-Sa , Wang Xun-Heng , Zhao Mei-Ting
Published: August 2024

Attention deficit hyperactivity disorder (ADHD) is one of the most common neurodevelopmental disorders in childhood. Numerous resting-state functional magnetic resonance imaging (rs-fMRI) studies in ADHD have been performed using traditional low-frequency bands (0.01–0.08 Hz). However, the neural activity patterns of frequency subbands in ADHD still require further investigation. The purpose of this study is to explore the frequency-dependent characteristics and neural activity patterns of ADHD subtypes. We selected the ADHD combined type (ADHD-C, N = 25), ADHD inattentive type (ADHD-I, N = 26) and typically developing (TD, N = 28) children from the ADHD-200 Consortium. Based on the slow-5 band (0.01–0.027 Hz) and slow-4 band (0.027–0.073 Hz), we generated static and dynamic fractional amplitude of low-frequency fluctuation (fALFF) and regional homogeneity (ReHo) maps for each participant. A flexible-factorial analysis of variance model was performed on static and temporal dynamic rs-fMRI measurements within two subbands. Results revealed that the orbital-frontal gyrus, precuneus, superior temporal gyrus and angular gyrus were found to have obvious frequency band and group interaction effects. The intrinsic neural activity differences among three groups were more prominent in the slow-5 frequency band compared to the slow-4 band. In addition, the indices of significant interaction regions showed correlations with the progression of the disease and the features in slow-5 showed an advantageous diagnostic performance compared with those in slow-4. The results suggested the intrinsic neural activities of ADHD subtypes were frequency-dependent. The frequency-specific analysis of static and dynamic brain activity may provide a deeper understanding of neurophysiological dysfunction patterns in ADHD subtypes and provide supplementary information for assessing ADHD subtypes.
Novel strategies in Parkinson’s disease treatment: a review
Authors: Mitchell Charles L. , Kurouski Dmitry
Published: August 2024

An unprecedented extension of life expectancy observed during the past century drastically increased the number of patients diagnosed with Parkinson’s diseases (PD) worldwide. Estimated costs of PD alone reached $52 billion per year, making effective neuroprotective treatments an urgent and unmet need. Current treatments of both AD and PD focus on mitigating the symptoms associated with these pathologies and are not neuroprotective. In this review, we discuss the most advanced therapeutic strategies that can be used to treat PD. We also critically review the shift of the therapeutic paradigm from a small molecule-based inhibition of protein aggregation to the utilization of natural degradation pathways and immune cells that are capable of degrading toxic amyloid deposits in the brain of PD patients.
Precision nutrition-based strategy for management of human diseases and healthy aging: current progress and challenges forward
Authors: Singh Vipin Kumar , Hu Xiao-Hu , Singh Amit Kishore , Solanki Manoj Kumar , Vijayaraghavan Pooja , Srivastav Rajpal , Joshi Naveen Kumar , Kumari Madhuree , Singh Sandeep Kumar , Wang Zhen , Kumar Ajay
Published: August 2024

Currently, the treatment of various human ailments is based on different therapeutic approaches including traditional and modern medicine systems. Precision nutrition has come into existence as an emerging approach considering the diverse aspects such as age, sex, genetic and epigenetic makeup, apart from the pathophysiological conditions. The continuously and gradually evolving disciplines of genomics about nutrition have elucidated the importance of genetic variations, epigenetic information, and expression of myriads of genes in disease progression apart from the involvement in modulating therapeutic responses. Further, the investigations have presented the considerable role of gut microbiota comprising of commensal and symbionts performing innumerable activities such as release of bioactive molecules, defense against pathogenic microbes, and regulation of immunity. Noteworthy, the characteristics of the microbiome change depending on host attributes, environmental factors, and habitat, in addition to diet, and therefore can be employed as a biomarker to unravel the response to given food. The specific diet and the components thereof can be suggested for supporting the enrichment of the desired microbial community to some extent as an important part of precision nutrition to achieve not only the goal of human health but also of healthy aging.
Revisiting the potential of regulated cell death in glioma treatment: a focus on autophagy-dependent cell death, anoikis, ferroptosis, cuproptosis, pyroptosis, immunogenic cell death, and the crosstalk between them
Authors: Luo Maowen , Luan Xingzhao , Yang Chaoge , Chen Xiaofan , Yuan Suxin , Cao Youlin , Zhang Jing , Xie Jiaying , Luo Qinglian , Chen Ligang , Li Shenjie , Xiang Wei , Zhou Jie
Published: August 2024

Gliomas are primary tumors that originate in the central nervous system. The conventional treatment options for gliomas typically encompass surgical resection and temozolomide (TMZ) chemotherapy. However, despite aggressive interventions, the median survival for glioma patients is merely about 14.6 months. Consequently, there is an urgent necessity to explore innovative therapeutic strategies for treating glioma. The foundational study of regulated cell death (RCD) can be traced back to Karl Vogt’s seminal observations of cellular demise in toads, which were documented in 1842. In the past decade, the Nomenclature Committee on Cell Death (NCCD) has systematically classified and delineated various forms and mechanisms of cell death, synthesizing morphological, biochemical, and functional characteristics. Cell death primarily manifests in two forms: accidental cell death (ACD), which is caused by external factors such as physical, chemical, or mechanical disruptions; and RCD, a gene-directed intrinsic process that coordinates an orderly cellular demise in response to both physiological and pathological cues. Advancements in our understanding of RCD have shed light on the manipulation of cell death modulation - either through induction or suppression - as a potentially groundbreaking approach in oncology, holding significant promise. However, obstacles persist at the interface of research and clinical application, with significant impediments encountered in translating to therapeutic modalities. It is increasingly apparent that an integrative examination of the molecular underpinnings of cell death is imperative for advancing the field, particularly within the framework of inter-pathway functional synergy. In this review, we provide an overview of various forms of RCD, including autophagy-dependent cell death, anoikis, ferroptosis, cuproptosis, pyroptosis and immunogenic cell death. We summarize the latest advancements in understanding the molecular mechanisms that regulate RCD in glioma and explore the interconnections between different cell death processes. By comprehending these connections and developing targeted strategies, we have the potential to enhance glioma therapy through manipulation of RCD.
Assessing the immunogenicity risk of salmon calcitonin peptide impurities using in silico and in vitro methods
Authors: Roberts Brian J. , Mattei Aimee E. , Howard Kristina E. , Weaver James L. , Liu Hao , Lelias Sandra , Martin William D. , Verthelyi Daniela , Pang Eric , Edwards Katie J. , De Groot Anne S.
Published: August 2024

Advances in synthetic peptide synthesis have enabled rapid and cost-effective peptide drug manufacturing. For this reason, peptide drugs that were first produced using recombinant DNA (rDNA) technology are now being produced using solid- and liquid-phase peptide synthesis. While peptide synthesis has some advantages over rDNA expression methods, new peptide-related impurities that differ from the active pharmaceutical ingredient (API) may be generated during synthesis. These impurity byproducts of the original peptide sequence feature amino acid insertions, deletions, and side-chain modifications that may alter the immunogenicity risk profile of the drug product. Impurities resulting from synthesis have become the special focus of regulatory review and approval for human use, as outlined in the FDA’s Center for Drug Evaluation and Research guidance document, “ANDAs for Certain Highly Purified Synthetic Peptide Drug Products That Refer to Listed Drugs of rDNA Origin,” published in 2021. This case study illustrates how in silico and in vitro methods can be applied to assess the immunogenicity risk of impurities that may be present in synthetic generic versions of the salmon calcitonin (SCT) drug product. Sponsors of generic drug abbreviated new drug applications (ANDAs) should consider careful control of these impurities (for example, keeping the concentration of the immunogenic impurities below the cut-off recommended by FDA regulators). Twenty example SCT impurities were analyzed using in silico tools and assessed as having slightly more or less immunogenic risk potential relative to the SCT API peptide. Class II human leukocyte antigen (HLA)-binding assays provided independent confirmation that a 9-mer sequence present in the C-terminus of SCT binds promiscuously to multiple HLA DR alleles, while T-cell assays confirmed the expected T-cell responses to SCT and selected impurities. In silico analysis combined with in vitro assays that directly compare the API to each individual impurity peptide may be a useful approach for assessing the potential immunogenic risk posed by peptide impurities that are present in generic drug products.
Impact of changes in antihypertensive medication on treatment intensity at hospital discharge and 30 days afterwards
Authors: Japelj Nuša , Kerec Kos Mojca , Jošt Maja , Knez Lea
Published: August 2024

Introduction

Little is known about the cumulative effect of changes in antihypertensive medications on treatment intensity. This study analyzed how changes in antihypertensive medications affect the intensity of antihypertensive treatment at hospital discharge and 30 days afterwards.

Methods

A prospective observational study of 299 hospitalized adult medical patients with antihypertensive therapy was conducted. The effect of medication changes on treatment intensity was evaluated by the Total Antihypertensive Therapeutic Intensity Score (TIS).

Results

At discharge, antihypertensive medications were changed in 62% of patients (184/299), resulting in a very small median reduction in TIS of −0.16. Treatment intensity was reduced more with increasing number of antihypertensive medications at admission, whereas it increased with elevated inpatient systolic blood pressure. Thirty days after discharge, antihypertensive medications were changed in 37% of patients (88/239) resulting in a median change in TIS of −0.02. Among them, 90% (79/88) had already undergone a change at discharge. The change in treatment intensity after discharge was inversely correlated with a change at discharge.

Discussion

Changes in antihypertensive medication frequently occurred at discharge but had a minimal impact on the intensity of antihypertensive treatment. However, these adjustments exposed patients to further medication changes after discharge, evidencing the need for treatment reassessment in the first month post-discharge.
Phosphoenolpyruvate carboxykinase 2-mediated metabolism promotes lung tumorigenesis by inhibiting mitochondrial-associated apoptotic cell death
Authors: Zhang Jing , He Wenjuan , Liu Dongmei , Zhang Wenyu , Qin Huan , Zhang Song , Cheng Ailan , Li Qiang , Wang Feilong
Published: August 2024

Background

It is unknown how cancer cells override apoptosis and maintain progression under nutrition-deprived conditions within the tumor microenvironment. Phosphoenolpyruvate carboxykinase (PEPCK or PCK) catalyzes the first rate-limiting reaction in gluconeogenesis, which is an essential metabolic alteration that is required for the proliferation of cancer cells under glucose-limited conditions. However, if PCK-mediated gluconeogenesis affects apoptotic cell death of non small cell lung cancer (NSCLC) and its potential mechanisms remain unknown.

Methods

RNA-seq, Western blot and RT-PCR were performed in A549 cell lines cultured in medium containing low or high concentrations of glucose (1 mM vs. 20 mM) to gain insight into how cancer cells rewire their metabolism under glucose-restriction conditions. Stable isotope tracing metabolomics technology (LC-MS) was employed to allow precise quantification of metabolic fluxes of the TCA cycle regulated by PCK2. Flow Cytometry was used to assess the rates of early and later apoptosis and mitochondrial ROS in NSCLC cells. Transwell assays and luciferase-based in vivo imaging were used to determine the role of PCK2 in migration and invasion of NSCLC cells. Xenotransplants on BALB/c nude mice to evaluate the effects of PCK2 on tumor growth in vivo. Western blot, Immunohistochemistry and TUNEL assays to evaluate the protein levels of mitochondrial apoptosis.

Results

This study report that the mitochondrial resident PCK (PCK2) is upregulated in dependent of endoplasmic reticulum stress-induced expression of activating transcription factor 4 (ATF4) upon glucose deprivation in NSCLC cells. Further, the study finds that PCK2-mediated metabolism is required to decrease the burden of the TCA cycles and oxidative phosphorylation as well as the production of mitochondrial reactive oxygen species. These metabolic alterations in turn reduce the activation of Caspase9-Caspase3-PARP signal pathway which drives apoptotic cell death. Importantly, silencing PCK2 increases apoptosis of NSCLC cells under low glucose condition and inhibits tumor growth both in vitro and in vivo.

Conclusion

In summary, PCK2-mediated metabolism is an important metabolic adaptation for NSCLC cells to acquire resistance to apoptosis under glucose deprivation.
Effect of eight-week high-intensity interval training versus moderate-intensity continuous training programme on body composition, cardiometabolic risk factors in sedentary adolescents
Authors: Sun Fucheng , Williams Craig A. , Sun Qiang , Hu Feng , Zhang Ting
Published: August 2024

Objectives

This study aimed to assess and compare the effect of an 8-week high-intensity interval training (HIIT) or moderate-intensity continuous training (MICT) programme on body composition and cardiovascular metabolic outcomes of sedentary adolescents in China.

Methods

Eighteen sedentary normal-weight adolescents (age: 18.5 ± 0.3 years, 11 females) were randomized into three groups. HIIT group protocol consisted of three sessions/week for 8-week of “all out” sprints to reach 85%–95% of HRmax, and MICT group protocol undertook three sessions/week for 8-week of continuous running to reach 65%–75% of HRmax. The control group resumed normal daily activities without any intervention. Blood pressure and body composition were measured, and fasting blood samples were obtained at baseline and 48 h post-trial. Mixed-design ANOVA analysis was employed followed by post hoc t-tests and Bonferroni alpha-correction was used to evaluate interaction, between-group, and within-group differences, respectively.

Results

Results indicated that HIIT and MICT similarly affected body fat mass (p = 0.021, ES = 0.19; p = 0.016, ES = 0.30, respectively), body fat percentage (p = 0.037, ES = 0.17; p = 0.041, ES = 0.28, respectively), visceral fat area (p = 0.001, ES = 0.35; p = 0.003, ES = 0.49, respectively) of body composition. A positive outcome was observed for waist/hip ratio (p = 0.033, ES = 0.43) in HIIT, but not MICT (p = 0.163, ES = 0.33). No significant differences were found between groups for any clinical biomarkers. However, pairwise comparison within the group showed a significant decrease in systolic blood pressure (p = 0.018, ES = 0.84), diastolic blood pressure (p = 0.008, ES = 1.76), and triglyceride (p = 0.004, ES = 1.33) in HIIT, but no significant differences were found in the MICT and Control group.

Conclusion

Both 8-week HIIT and MICT programmes have similar positive effects on reducing body fat mass, fat percentage, and visceral fat area. However, sedentary adolescents may have limited scope to decrease insulin resistance after these 8-week interventions. Notably, the 8-week HIIT intervention was highly effective in increasing cardiometabolic health compared to the MICT. The exercise intensity threshold value and metabolic outcomes of high-intensity interval sprints should be explored further to extend the long-term benefit in this cohort.
Prevalence of sickness presenteeism and associated factors among primary school teachers in Gondar city, northwest Ethiopia
Authors: Mamaye Yimer , Yenealem Dawit Getachew , Fentanew Molla , Abebaw Tadiwos , Melaku Christian , Bezie Anmut Endalkachew , Abie Alebachew Bitew , Tesfaye Amensisa Hailu
Published: August 2024

Background

Sickness presenteeism, the phenomenon of people going to work despite being ill, is an occupational and psychosocial condition that hurts both the health of workers and organizational productivity. It negatively affects health, increases health-related costs, and the risk of contagious diseases. Primary school teachers are particularly vulnerable to this problem, although little is known about its scope and associated factors. This study aimed to determine the prevalence and factors associated with sickness presenteeism among school teachers in Northwest Ethiopia.

Methods

An institution-based cross-sectional study was employed and the study period was from April 18 to May 18, 2023. A sample of 633 primary school teachers was recruited using two-stage stratified random sampling. Data were collected using structured self-administered questionnaires. Epi-data version 4.6 and STATA version 14 were used for data entry and analysis, respectively. Binary logistic regression analysis was used. A multivariable logistic regression model with an adjusted odds ratio was fitted for statistical significance.

Results

A total of 603 school teachers participated in this study, with a response rate of 95.26%. The overall prevalence of sickness presenteeism in the last 12 months was 54.7% (N = 330) [95% CI (50.9, 58.7)]. Private school teachers [AOR: 2.21, 95% CI (1.14, 4.28)], low supervisor support [AOR: 1.53, 95% CI (1.06, 2.20)], lack of staff replacement availability [AOR: 2.74, 95% CI (1.85, 4.06)], low colleague support [AOR: 2.17, 95% CI (1.40, 3.37)], unsuitable household conditions [AOR: 1.49, 95% CI (1.08, 2.34)], and strict attendance control [AOR: 2.54, 95% CI (1.67, 3.85)] were factors significantly associated with sickness presenteeism.

Conclusion

The prevalence of sickness presenteeism was relatively high among primary school teachers because of factors such as low support from supervisors and colleagues, strict attendance control, lack of staff replacement, unsuitable household conditions, and private school type. Strategies to promote teachers’ health include fostering a culture of support and collaboration among colleagues, recruiting adequate staff, and implementing liberal attendance policies.
Disparities in all-cause mortality among people experiencing homelessness in Toronto, Canada during the COVID-19 pandemic: a cohort study
Authors: Richard Lucie , Carter Brooke , Wu Linda , Hwang Stephen W.
Published: August 2024

People experiencing homelessness have historically had high mortality rates compared to housed individuals in Canada, a trend believed to have become exacerbated during the COVID-19 pandemic. In this matched cohort study conducted in Toronto, Canada, we investigated all-cause mortality over a one-year period by following a random sample of people experiencing homelessness (n = 640) alongside matched housed (n = 6,400) and low-income housed (n = 6,400) individuals. Matching criteria included age, sex-assigned-at-birth, and Charlson comorbidity index. Data were sourced from the Ku-gaa-gii pimitizi-win cohort study and administrative databases from ICES. People experiencing homelessness had 2.7 deaths/100 person-years, compared to 0.7/100 person-years in both matched unexposed groups, representing an all-cause mortality unadjusted hazard ratio (uHR) of 3.7 (95% CI, 2.1–6.5). Younger homeless individuals had much higher uHRs than older groups (ages 25–44 years uHR 16.8 [95% CI 4.0–70.2]; ages 45–64 uHR 6.8 [95% CI 3.0–15.1]; ages 65+ uHR 0.35 [95% CI 0.1–2.6]). Homeless participants who died were, on average, 17 years younger than unexposed individuals. After adjusting for number of comorbidities and presence of mental health or substance use disorder, people experiencing homelessness still had more than twice the hazard of death (aHR 2.2 [95% CI 1.2–4.0]). Homelessness is an important risk factor for mortality; interventions to address this health disparity, such as increased focus on homelessness prevention, are urgently needed.
The nexus between Helicobacter pylori infection and anemia—a systematic review
Authors: Motupalli Sankeerth Kumar , Oroszi Terry L.
Published: August 2024

This systematic review delves into the intricate relationship between Helicobacter pylori (H. pylori) infection and anemia, shedding light on its significance for public health. Helicobacter pylori, a prevalent bacterium associated with gastritis, peptic ulcers, and stomach cancer, is examined alongside anemia, characterized by a deficiency in red blood cells or hemoglobin. Through an exploration of epidemiology, pathophysiological mechanisms, and healthcare implications, this review emphasizes the global distribution and incidence rates of H. pylori infections and anemia. Socioeconomic and environmental factors influencing prevalence are underscored. Diagnostic methodologies and treatment options for both conditions are discussed in detail. Analysis of studies investigating the causal relationship between H. pylori infection and anemia reveals potential mechanisms such as inflammation-induced alterations in iron and vitamin B12 absorption. While evidence suggests a connection between H. pylori infection and anemia, methodological limitations are acknowledged, necessitating further research to establish causality conclusively. The review highlights the importance of healthcare practitioners considering H. pylori screening for patients with unexplained or persistent anemia symptoms, as effective treatment of H. pylori infection may lead to improved anemia outcomes. Challenges such as antibiotic resistance and patient adherence to treatment regimens are identified. In conclusion, ongoing research into the link between H. pylori infection and anemia offers promising insights, albeit with persisting gaps in understanding. Collaborative efforts are required to address methodological challenges and develop tailored prevention and treatment strategies, accounting for variations in prevalence across populations and regions.
Promoting mental wellbeing among youth Australian Rules footballers through a model of continuous improvement
Authors: Couston Nicky , Hoare Erin , Hall Kate
Published: August 2024

The developmental period of adolescence is a known transitional life phase with unique risk and protective factors that can affect mental wellbeing outcomes. This, in combination with the pressures and demand of elite sport, make young elite athletes an important population in which positive mental wellbeing can be explored. This study aimed to examine the state of wellbeing, informed by positive psychology and the Positive emotions, Engagement, Relationships, Meaning, Accomplishment (PERMA) model of wellbeing, in a cohort of young athletes aged 16–19 years in the Australian setting as part of a model of continuous improvement. The objectives of these aims were to inform the development of a wellbeing curriculum for implementation in the Australian Rules Football talent pathway and to assess whether wellbeing presents differently in young athletes relative to the general school-attending population of that same age. Participants were 608 young Australian footballers participating in the developmental talent pathway during 2020 and 2021. There were 299 young male footballers, and 309 young women footballers who completed data collection. Wellbeing was assessed using the Engagement, Perseverance, Optimism, Connectedness, Happiness Measure of Adolescent Wellbeing (EPOCH) which corresponds to the PERMA model of wellbeing, and total mean scores were reported. Engagement and connectedness were similar for the young men and women athletes. Young men reported higher perseverance, optimism, happiness, and overall wellbeing relative to young women. Wellbeing among young athletes appears similar to the general population, however perseverance may be higher among young athletes. These findings form an important component of the continuous improvement model adopted in the football program in that the results informed the development of a tailored wellbeing curriculum program that is reflective of the wellbeing needs of the young athletes.
Unlocking the potential of de-oiled seeds of Citrus sinensis loaded with metal nanoparticles for Congo red degradation and removal: a green water treatment strategy with bibliometric analysis
Authors: Tahira Misbah , Batool Fozia , Noreen Sobia , Mustaqeem Muhammad , Munawar Khurram Shahzad , Kanwal Samia , Shahbaz Komal , Arshad Anila , Ali Hayssam M.
Published: August 2024

This research reported the utilization of novel adsorbent from the de-oiled waste material of orange seeds for preparing simple charcoal (SC) and iron oxide/activated charcoal (Fe2O3/AC) nanocomposites. Batch adsorption experiments were carried out to evaluate the optimized conditions of the experiment. The results obtained indicated that the pseudo-second-order kinetic model best fitted the adsorption data (R2 > 0.99) and that the Freundlich isotherm model best explained the adsorption of dye on Fe2O3/AC. The adsorption process was spontaneous and exothermic in the temperature range of 293–333°K, as explained by calculated thermodynamic parameters, e.g., ΔG°, ΔH°, and ΔS°. The Fourier transform infrared (FTIR) spectroscopy results publicized that carboxyl and amine functional groups are present on the surface of adsorbents, which are responsible for the attachment of dye. The scanning electron microscopy (SEM) results showed that Fe2O3/AC has a porous surface and textual structure, which can efficiently adsorb dye molecules. A zetasizer was utilized for determining the size of the composites, and the thermal stability was determined by performing a thermogravimetric analysis (TGA). The findings of the comparative experiment indicated that Fe2O3/AC are more promising than raw activated carbon for the adsorption of Congo red (CR). The impregnation of iron oxide nanocomposites on an adsorbent resulted in an increased surface area-to-volume ratio, magnetic properties, and excellent reusable capacity. Overall, it can be reported as an innovative procedure promoting the recycling of waste products, which aids in protecting environmental and human health and in the development of the economy.
A proposed new Tombusviridae genus featuring extremely long 5’ untranslated regions and a luteo/polerovirus-like gene block
Authors: Lozier Zachary , Hill Lilyahna , Semmann Elizabeth , Miller W. Allen
Published: August 2024

Tombusviridae is a large family of single-stranded, positive-sense RNA plant viruses with uncapped, non-polyadenylated genomes encoding 4–7 open reading frames (ORFs). Previously, we discovered, by high-throughput sequencing of maize and teosinte RNA, a novel genome of a virus we call Maize-associated tombusvirus (MaTV). Here we determined the precise termini of the MaTV genome by using 5’ and 3’ rapid amplification of cDNA ends (RACE). In GenBank, we discovered eleven other nearly complete viral genomes with MaTV-like genome organizations and related RNA-dependent RNA polymerase (RdRp) sequences. These genomes came from diverse plant, fungal, invertebrate and vertebrate organisms, and some have been found in multiple organisms across the globe. The available 5’ untranslated regions (UTRs) of these genomes are remarkably long: at least 438 to 727 nucleotides (nt), in contrast to those of other tombusvirids, which are <150 nt. Moreover these UTRs contain 6 to 12 AUG triplets that are unlikely to be start codons, because - with the possible exception of MaTV - there are no large or conserved ORFs in the 5’ UTRs. Such features suggest an internal ribosome entry site (IRES), but the only conserved features we found were that the 50 nt upstream of and adjacent to the ORF1 start codon are cytosine-rich and guanosine-poor. ORF2 (RdRp gene) appears to be translated by in-frame ribosomal readthrough of the ORF1 stop codon. In all twelve genomes we identified RNA structures known in other tombusvirids to facilitate this readthrough. ORF4 overlaps with ORF3 (coat protein gene) and may initiate with a non-AUG start codon. ORF5 is predicted to be translated by readthrough of the ORF3 stop codon. The proteins encoded by ORFs 4 and 5 diverge highly from each other and from those of the similarly organized luteo- and poleroviruses. We also found no obvious 3’ cap-independent translation elements, which are present in other tombusvirids. The twelve genomes diverge sufficiently from other tombusvirids to warrant classification in a new genus. Because they contain two leaky stop codons and a potential leaky start codon, we propose to name this genus Rimosavirus (rimosa = leaky in Latin).
Dietary Knowledge, Attitudes, and Behaviors Related to Obesity and Highly Underweight Among Urban Chinese High School Students: A Cross-Sectional Study
Authors: Huo Yujia , Monma Takafumi , Kataoka Chie , Takeda Fumi
Published: August 2024

Objectives

This study aimed to identify dietary knowledge, attitudes, and behaviors related to obesity and highly underweight among urban Chinese high school students.

Methods

Using the data of 403 high school students from a cross-sectional survey in 2022, multinomial logistic regression analysis was conducted with the body mass index as the objective variable (reference: normal weight), dietary knowledge, attitudes, and behaviors as the explanatory variables, adjusted for sex.

Results

Both obesity and highly underweight were most strongly related to incorrect dietary knowledge of desirable types of diets. Additionally, obesity was related to inappropriate dietary attitudes regarding the importance of diet, eating at irregular meal-times, and eating without chewing well, while highly underweight was related to picky eating and not having three meals per day, but not related to attitudes.

Conclusion

The incorrect knowledge of desirable type of diet was related to both obesity and highly underweight, while other risk factors of knowledge, attitudes, and behaviors related to obesity or highly underweight differed respectively. These findings should be useful in examining effective nutrition education for urban Chinese high school students.
Numerical Study on Far-Field Noise Characteristic Generated by Wall-Mounted Swept Finite-Span Airfoil within Transonic Flow
Authors: Jiang Runpei , Liu Peiqing , Zhang Jin , Guo Hao
Published: August 2024

This study seeks to develop a fundamental comprehension of the noise challenges encountered by commercial aircraft fuselage surface attachments, such as blade antennas and pitot tubes. The study examines the flow characteristics and far-field noise directivity of a wall-mounted NACA0012 airfoil with various sweep angles (−35°, −15°, 0°, +15°, and +35°) and an aspect ratio of 1.5. The Mach numbers of the incoming flow range from 0.8 to 0.9 with a Reynolds number of about 7 × 105. Delayed Detached Eddy Simulation (DDES) and the Ffowcs Williams–Hawkings (FW-H) equation are utilized. The results show that the shock wave intensity at the junction between the airfoil and the bottom wall is enhanced by the forward-swept angle. The shock wave moves and changes into a λ-type structure, while the boundary layer separates and produces shedding vortices in the junction at a smaller Mach number on the forward-swept airfoil compared to the straight airfoil and the backward-swept airfoil. These phenomena cause significant surface pressure fluctuations in the junction and result in a significant dipole noise in the far field, which is the primary source of noise in the far field. In addition, the normal Mach number and the absolute sweep angle also contribute to the far-field noise.
Farmers’ Willingness to Engage in Ecological Compensation for Crop Rotation in China’s Black Soil Regions
Authors: Xue Ruhao , Faye Bonoua , Zhang Rui , Gong Xin , Du Guoming
Published: August 2024

Assessments of farmer satisfaction can be a crucial tool for effectively implementing an appropriate ecological compensation policy. This study evaluates the effectiveness of an ecological compensation policy for crop rotation in China’s black soil regions from the perspective of farmer satisfaction. Specifically, utilizing an improved entropy TOPSIS, logistic regression, and the obstacle degree model, this empirical study analyzes the performance of the ecological compensation policy for crop rotation in black soil regions and identifies barriers in Heilongjiang Province. The findings indicate that gender significantly and positively affects outcomes at the 10% level, while age has a notable influence at the 5% level. Additionally, the family labor force and the quality of cultivated land, both significant at the 5% level with negative coefficients, suggest a diminished likelihood of farmers participating in these ecological compensation projects. The family’s source of income, significantly influential at the 1% level, also indicates a lower propensity among farmers to engage. Performance analysis reveals that the values for variables of satisfaction with the project’s publicity (A1), satisfaction with the protection of farmers’ rights and interest (A3), overall satisfaction with the effectiveness of the project (B1), satisfaction with ecological compensation methods (B3), and satisfaction with household income after the implementation of the project (C1) are above the average. In contrast, values for variables of satisfaction with the supervision and management (A2), satisfaction with the payment of ecological compensation funds (A4), satisfaction level with black soil rotation ecological compensation standards (B2), satisfaction with the welfare level of family life after the implementation of the project (C2), and satisfaction with farmers’ proactive participation in the project (D1) fall below the average of 3.03. Therefore, this study provides a comprehensive framework for assessing the effectiveness of the ecological compensation policy for crop rotation in China’s black soil regions and offers recommendations for enhancing its performance.
Erythrocyte Oxidative Status in People with Obesity: Relation to Tissue Losses, Glucose Levels, and Weight Reduction
Authors: Szlachta Beata , Birková Anna , Čižmárová Beáta , Głogowska-Gruszka Anna , Zalejska-Fiolka Paulina , Dydoń Maria , Zalejska-Fiolka Jolanta
Published: August 2024

Background: This study aimed to investigate the impact of reductions in various body mass components on the erythrocyte oxidative status and glycemic state of people with obesity (PWO). Methods: A total of 53 PWO followed a six-month individualized low-calorie diet with exercise, during which anthropometric, biochemical, and oxidative parameters were measured. The participants were divided into groups based on weight (W), visceral fat area (VFA), total body water (TBW), and skeletal muscle mass (SMM) losses, as well as normoglycemia (NG) and hyperglycemia (HG). Results: Weight reduction normalized glycemia and influenced erythrocyte enzyme activity. Regardless of the tissue type lost (VFA, TBW, or SMM), glutathione peroxidase activity decreased in all groups, accompanied by an increase in glutathione reductase activity. Lipofuscin (LPS) and malondialdehyde (MDA) concentrations decreased regardless of the type of tissue lost. The α-/γ-tocopherol ratio increased in those losing >10% body weight, >15% VFA, and >5% TBW. In the NG group, compared to the HG group, there was a decrease in glutathione peroxidase and an increase in glutathione reductase, with these changes being stronger in the HG group. The LPS and MDA concentrations decreased in both groups. Significant correlations were observed between glucose reduction and changes in catalase, retinol, and α-tocopherol, as well as between VFA reduction and changes in vitamin E, L-LPS, and the activities of L-GR and L-GST. Conclusions: This analysis highlights the complex interactions between glucose metabolism, oxidative state, and erythrocyte membrane integrity, crucial for understanding diabetes and its management. This study shows the significant metabolic adaptability of erythrocytes in response to systemic changes induced by obesity and hyperglycemia, suggesting potential therapeutic targets to improve metabolic health in obese individuals.
Extraction Optimization, Structural Analysis, and Potential Bioactivities of a Novel Polysaccharide from Sporisorium reilianum
Authors: Shi He , Zhang Siyi , Zhu Mandi , Li Xiaoyan , Jie Weiguang , Kan Lianbao
Published: August 2024

Sporisorium reilianum is an important biotrophic pathogen that causes head smut disease. Polysaccharides extracted from diseased sorghum heads by Sporisorium reilianum exhibit significant medicinal and edible value. However, the structure and biological activities of these novel polysaccharides have not been explored. In this study, a novel polysaccharide (WM-NP’-60) was isolated and purified from the fruit bodies of S. reilianum and aimed to explore the structural characteristics and substantial antioxidant and antitumor properties of WM-NP’-60. Monosaccharide composition determination, periodate oxidation-Smith degradation, 1D/2D-NMR analysis, and methylation analysis revealed that WM-NP’-60 consisted mainly of β-1,6-D-Glcp, β-1,3-D-Glcp, and β-1,3,6-D-Glcp linkages. The antioxidant assays demonstrated that WM-NP’-60 exhibited great activities, including scavenging free radicals, chelating ferrous ions, and eliminating reactive oxygen species (ROS) within cells. The HepG2, SGC7901, and HCT116 cells examined by transmission electron microscopy (TEM) revealed typical apoptotic bodies. Therefore, a novel fungal polysaccharide (WM-NP’-60) was discovered, extracted, and purified in this experiment, with the aim of providing a reference for the development of a new generation of food and nutraceutical products suitable for human consumption.
Exploring Plasma Coenzyme Q10 Status in Paediatric Dyslipidaemia
Authors: Minguez Beatriz , de Los Santos Mariela , Garcia-Volpe Camila , Molera Cristina , Paredes-Fuentes Abraham J. , Oliva Clara , Arias Angela , Rodriguez-Gonzalez Helena , Yubero Delia , Tondo Mireia , Santos-Ocaña Carlos , Meavilla Silvia , Artuch Rafael
Published: August 2024

Coenzyme Q10 (CoQ) is a ubiquitous lipid with different biological functions. In blood, there is a close relationship between CoQ status and cholesterol, which strongly supports the study of both molecules simultaneously. The objective of this study was to evaluate plasma CoQ, lipoprotein concentrations and CoQ/Chol ratio in a cohort of paediatric patients with different types of dyslipidaemias. A total of 60 paediatric patients were recruited (age range: 7 months–18 years), including 52 with different types of hypercholesterolemia, 2 with isolated hypertriglyceridemia and 6 with hypobetalipoproteinemia. Plasma CoQ was analysed by HPLC with electrochemical detection, and lipoprotein and cholesterol concentrations by standard automated methods. The lowest CoQ values were detected in patients with hypobetalipoproteinemia and in two cases of liver cirrhosis. Mean CoQ values were significantly higher in hypercholesterolemic patients compared to controls (average values 1.07 µmol/L and 0.63 µmol/L) while the CoQ/cholesterol ratio did not show differences (170 vs. 163, respectively). Mean CoQ values were significantly lower in the group of patients with hypobetalipoproteinemia compared to controls (mean CoQ values of 0.22 µmol/L vs. 0.63 µmol/L, respectively), while those of CoQ/cholesterol did not show differences. Pearson’s correlation test showed a positive correlation between the CoQ and cholesterol values (r = 0.565, p < 0.001) and between the CoQ and the LDL cholesterol values (r = 0.610, p < 0.001). Our results suggest that it is advisable to analyse plasma CoQ and cholesterol concentrations in patients with hypobetalipoproteinemia and hypercholesterolemia associated with liver damage.
Simulation and Catastrophe Detection of Spontaneous Combustion Processes in Sulfide Ores
Authors: Pan Wei , Wang Shuo , Yi Ruge , Kang Youqing
Published: August 2024

Spontaneous combustion of sulfide ores during mining can lead to severe fires. To explore the transformation of state in the whole process of spontaneous combustion of sulfide ores, the simulation experiment of the whole unsteady process of spontaneous combustion of sulfide ore heap was carried out, and the most appropriate wavelet function was selected, combined with catastrophe detection and other methods for data mining and processing. The results indicate spatial differences in the response of the ore heap to environmental temperature changes during the whole unsteady process of spontaneous combustion of the sulfide ore heap. The reaction in the area near the surface of the heap is more prominent and faster, and the response in the area near the center of the heap is longer in duration. Moreover, there must be at least one catastrophe point in this process, and the catastrophe temperature must be between 108.2 °C and 113.9 °C. Finally, the whole unsteady process of the spontaneous combustion of the sulfide ore heap can be divided into four regions. Among them, region (II) is in a stage of obvious self-heating/near spontaneous combustion, and it is the catastrophe stage.