bluuebunny/crossref_metadata_2025 huggingface dataset:
Fear, trust, and compliance with COVID-19 measures: a study of the mediating effect of trust in government on the relationship between fear and compliance
Authors: Hrbková Lenka , Kudrnáč Aleš
Published: March 2024

As the COVID-19 pandemic became an unprecedented global threat, it was accompanied by an increase in trust in governments as well as fear among the public. Previous research suggests that both institutional trust and fear contribute to the willingness of citizens to comply with anti-pandemic measures. Moreover, fear during the contagion also increases trust in government. This article presents a test of the mediation of the effects of fear on compliance through trust. In addition, it differentiates between three different facets of COVID-19-related fear: fear of the disease, fear of economic consequences, and fear of political consequences. The results suggest that while fear of the disease increases compliance, fear of political consequences decreases compliance. Moreover, the effects of fear are mediated through trust in government. The negative impact of fear of political consequences on compliance increased between April and December 2020.
Impact of talker variability on language development in two-year-olds
Authors: Zhao Jing , Kobayashi Tessei , Haryu Etsuko
Published: March 2024

This research investigated the impact of the number of talkers with whom children engage in daily conversation on their language development. Two surveys were conducted in 2020, targeting two-year-olds growing up in Japanese monolingual families. Caregivers reported the number of talkers in three age groups and children’s productive vocabulary via questionnaires. The results demonstrated significant effects of variability in talkers in fifth grade or above in Study 1 (N = 50; male = 23; r = .372) and in adult talkers in Study 2 (N = 175; non-nursery going; male = 76; r = .184) on children’s vocabulary development, after controlling for language exposure time and demographic variables. Possible mediating factors are discussed. This research extends previous findings from immigrant bilingual children to monolingual speakers in Japan, suggesting the potential contribution of available talkers other than caregivers in conversational environments.
How Populism Affects Bioethics
Authors: Ortiz-Millán Gustavo
Published: March 2024

This article aims at raising awareness about the intersection of populism and bioethics. It argues that illiberal forms of populism may have negative consequences on the evolution of bioethics as a discipline and on its practical objectives. It identifies at least seven potential negative effects: (1) The rise of populist leaders fosters “epistemological populism,” devaluing the expert and scientific perspectives on which bioethics is usually based, potentially steering policies away from evidence-based foundations. (2) The impact of “moral populism” is evident in legislative prioritization of the “morality of common people,” often solicited through popular consultations on issues like abortion, drug legalization, or LGBT issues. (3) Populist distrust in autonomous governmental agencies and advisory bodies, including national bioethics commissions, can compromise expert advice, challenging both their authority and decisions. (4) Populists may erode transparency by undermining institutions responsible for it, hindering access to vital information for bioethical research. (5) “Medical populism” creates adversarial dynamics, prompting politicians to make simplistic healthcare policy decisions based on political rather than informed criteria, adversely affecting vulnerable populations. (6) Radical-right populist parties’ “welfare chauvinism” may shape healthcare policies, impacting service access and resource allocation, disproportionately affecting vulnerable groups such as migrants, but indirectly affecting the rest of the population. (7) Nationalist sentiments associated with populism may obstruct international collaborations, posing challenges for global bioethics that seeks to address ethical concerns beyond national borders. In summary, these dynamics raise significant bioethical concerns encompassing evidence-based decision-making, transparency, healthcare equity, and global collaboration. How bioethicists may respond to these challenges is discussed.
The use of private regulatory measures to create healthy food retail environments: a scoping review
Authors: Dancey Jane , Reeve Belinda , Jones Alexandra , Ferguson Megan , van Burgel Emma , Brimblecombe Julie
Published: March 2024

Objective:

Different forms of public and private regulation have been used to improve the healthiness of food retail environments. The aim of this scoping review was to systematically examine the types of private regulatory measures used to create healthy food retail environments, the reporting of the processes of implementation, monitoring, review and enforcement and the barriers to and enablers of these.

Design:

Scoping review using the Johanna Briggs Institute guidelines. Ovid MEDLINE, PsycINFO, Embase, CINAHL Plus, Business Source Complete and Scopus databases were searched in October 2020 and again in September 2023 using terms for ‘food retail’, ‘regulation’ and ‘nutrition’. Regulatory measure type was described by domain and mechanism. Deductive thematic analysis was used to identify reported barriers and enablers to effective regulatory governance processes using a public health law framework.

Setting:

Food retail.

Participants:

Food retail settings using private regulatory measures to create healthier food retail environments.

Results:

In total, 17 694 articles were screened and thirty-five included for review from six countries, with all articles published since 2011. Articles reporting on twenty-six unique private regulatory measures cited a mix of voluntary (n 16), mandatory (n 6) measures, both (n 2) or did not disclose (n 2). Articles frequently reported on implementation (34/35), with less reporting on the other regulatory governance processes of monitoring (15/35), review (6/35) and enforcement (2/35).

Conclusions:

We recommend more attention be paid to reporting on the monitoring, review and enforcement processes used in private regulation to promote further progress in improving the healthiness of food retail environments.
Das Stück Brot ist wieder ein Stück Brot
Authors: Steiner Roth Sandra
Published: March 2024

Essstörungen haben immer vielfältige Ursachen, und es geht darum, dysfunktionalen Gedanken, Glaubenssätzen und inneren Haltungen auf die Spur zu kommen. Das Buch bietet eine Hilfestellung für Betroffene und Angehörige, die aus der langjährigen therapeutischen Arbeit der Autorin mit Frauen und Männern mit Essproblemen entstanden ist.
Das Ziel der Autorin ist es, Wege aufzuzeigen, wie Betroffene einen neuen Umgang mit dem Essen finden können und Stück für Stück zu einem gesunden Essverhalten zurückfinden.Das Buch gliedert sich in drei Teile:
1. Grundlegende und immer wiederkehrende Themen und Fragen, die der Autorin im Praxisalltag in den Gesprächen mit den Klient:innen mit Essstörungen begegnen, werden einfach verständlich und fachlich fundiert vorgestellt und erklärt. Begleitend werden die jeweils entsprechenden therapeutischen Interventionen beschrieben.
2. Persönlich verfasste Erfahrungsberichte von Betroffenen, von ehemals Betroffenen, Angehörigen, Partner:innenund Freund:innen, die das Thema aus ihrer persönlichen Perspektive beleuchten.
3. Kurze Fachinformationen zu den verschiedenen Formen von Essstörungen (Magersucht, Bulimie, Binge Eating Disorder).

„Ein wunderbares Buch, das Mut macht, ‚den Klippensprung zu wagen‘, wie eine Betroffene es nennt. Und es macht Hoffnung und zeigt Wege auf, diese Hoffnung in Leben umzusetzen.“
Dr. Bärbel Wardetzki, Praxis für Psychotherapie, Supervision und Coaching, München
Juhu, ich bekomme ein Geschwisterchen!
Authors: Rietzler Stefanie , Grolimund Fabian , Wilke Marcus
Published: March 2024

Ein Geschwisterchen?Ente Merle bekommt gleich acht!

Freudig bastelt sie mit Papa ein Mobile. Als die Küken endlich schlüpfen, ist Merle verzaubert. Im trubeligen Familienalltag entdeckt Merle, wie schön es ist, achtsam mit den Kleinen zu kuscheln, Mama und Papa beim Wickeln und Füttern zu helfen, ihre Geschwister zum Lachen zu bringen und gut auf sie aufzupassen.
Und zum Glück ist in Mamas und Papas Herz und auf ihrem Schoß genug Platz für sie alle!
Eine einfühlsame Vorlesegeschichte über das Abenteuer, zum großen Geschwisterkind zu werden, das Glück, auch als Große noch klein sein zu dürfen, und all die schönen Seiten einer starken Geschwisterbeziehung – von Anfang an.
Wohin, kleine Eda?
Authors: Olkowska Aneta , Ewald Stefanie , Kemmer Jule , Röder Laura Michelle
Published: March 2024

Einfühlsam werden in diesem Buch die Sorgen und Nöte eines Drachenmädchens dargestellt, das von seinen Eltern vernachlässigt wird.

Das Drachenmädchen Eda ist oft allein – ihre Eltern sind nicht für sie da. Aufgrund der Vernachlässigung durch ihre Eltern wird Eda in der Schule ausgegrenzt und fühlt sich allein. Aus Neugierde folgt sie einer Hummel, die ihr zeigt, wie man fliegt. Auf ihrer Reise trifft sie weitere Tiere, die ihr beibringen, was ein Drachenmädchen in ihrem Alter bereits können sollte. Mit ihren neu erlernten Fähigkeiten und dem Mut, den sie durch ihre Reise erhalten hat, erkennt Eda, dass sie Hilfe suchen kann, um ihre Probleme zu lösen. Zum ersten Mal seit langer Zeit fühlt sie sich stark und nicht mehr allein.

Das Kinderbuch „Wohin, kleine Eda?” ist ein Band aus der Reihe „Psychologische Kinderbücher“. Das Buch liefert Kindern, die von Vernachlässigung betroffen sind, sowie deren Umfeld wichtige Informationen sowie praktische Aufgaben und Übungen.

Die Arbeitsmaterialien zu diesem Buch können nach erfolgter Registrierung von der Hogrefe Website heruntergeladen werden.
A Pre‐Monsoon Signal of False Alarms of Indian Monsoon Droughts
Authors: Goswami Bidyut Bikash
Published: March 2024

Current knowledge suggests a drought Indian monsoon (perhaps a severe one) when the El Nino Southern Oscillation and Pacific Decadal Oscillation each exhibit positive phases (a joint positive phase). For the monsoons, which are exceptions in this regard, we found northeast India often gets excess pre‐monsoon rainfall. Further investigation reveals that this excess pre‐monsoon rainfall is produced by the interaction of the large‐scale circulation associated with the joint phase with the mountains in northeast India. We posit that a warmer troposphere, a consequence of excess rainfall over northeast India, drives a stronger monsoon circulation and enhances monsoon rainfall over central India. Hence, we argue that pre‐monsoon rainfall over northeast India can be used for seasonal monsoon rainfall prediction over central India. Most importantly, its predictive value is at its peak when the Pacific Ocean exhibits a joint positive phase and the threat of extreme drought monsoon looms over India.
Wildfire Smoke Directly Changes Biogenic Volatile Organic Emissions and Photosynthesis of Ponderosa Pines
Authors: Riches M. , Berg T. C. , Vermeuel M. P. , Millet Dylan B. , Farmer D. K.
Published: March 2024

Wildfires are increasing across the USA. While smoke events affect human exposure and air quality, wildfire smoke effects on ecosystem‐atmosphere interactions are poorly understood. We investigate smoke effects on biogenic volatile organic compound (VOC) emissions and photosynthesis for ponderosa pines. During several wildfire smoke events, we observed photosynthetic reduction with evidence for stomatal plugging and changes in leaf‐level uptake and emission of both biogenic and wildfire VOCs. During intense smoke events, photosynthesis and VOC emissions were almost entirely suppressed, but increased dramatically upon stomatal opening. We propose four types of VOC responses to this burst in stomatal opening: post‐burst emissions, pulsed emissions, surge emissions, and post‐burst uptake. Our observations suggest that wildfire smoke can affect plant physiology and leaf‐atmosphere gas exchange.
On the Role of Indian Ocean SST in Influencing the Differences in Atmospheric Variability Between 2020–2021 and 2021–2022 La Niña Boreal Winters
Authors: Zhang Tao , Kumar Arun
Published: March 2024

The difference in observed atmospheric anomalies over the Northern Hemisphere winter between 2021–22 and 2020–21 La Niña years indicated a tripole pattern consisting of a Japan cyclone, a Bering Sea anticyclone, and a cyclone over the North American continent. This feature, however, was not replicated in the North American Multi‐Model Ensemble (NMME) forecasts. A set of model sensitivity experiments was performed to better understand the cause of this discrepancy. The results revealed the possible role of the influence of sea surface temperature (SST) anomalies, particularly over the Indian Ocean, on the observed circulation differences that was further modulated by internal atmospheric variability. The failure in predicting circulation changes in NMME was next attributed to the errors in SST predictions over the Indian Ocean and highlights the need for improvements in SST forecasts over this region.
Low δ18O and δ30Si TTG at ca. 2.3 Ga Hints at an Intraplate Rifting Onset of the Paleoproterozoic Supercontinent Cycle
Authors: Zhou Yanyan , Zhai Mingguo , Mitchell Ross N. , Cawood Peter A. , Huang Guangyu , Spencer Christopher J. , Chen Mimi , Li Yibing , Zhao Taiping , Wu Tengfei
Published: March 2024

The start of the Paleoproterozoic supercontinent cycle is typically taken as the initiation of orogenesis at ca. 2.1 Ga leading to the assembly of Earth's first supercontinent, Columbia. However, the dearth of ca. 2.5–2.2 Ga geological records makes it difficult to deduce tectonic factors during the onset of the Paleoproterozoic supercontinent cycle. The petrogenesis of tonalite–trondhjemite–granodiorite (TTG) provides useful proxies for tracing prevailing geodynamic regimes of early continental evolution. However, marked decreases of TTG and other magmatism occurred across the Archean–Paleoproterozoic transition and have previously precluded forming testable hypotheses. Early Paleoproterozoic TTGs have been identified in the North China Craton (NCC) and other cratons, which may represent the last major pulse of TTGs globally. Here we present low δ18O and δ30Si ca. 2.3 Ga TTGs from the NCC, together with thermodynamic modeling and compilation of stable O and Si isotopes for TTGs globally through time. The ca. 2.3 Ga TTGs were derived from the partial melting of Archean basaltic crust and give lighter average zircon δ18O (3.15 ± 0.35‰) and whole‐rock δ30Si values (−0.17 ± 0.08‰) than most Archean TTGs. Considering coeval mafic‐felsic igneous rocks, and lithospheric thinning since ca. 2.5 Ga based on estimated crustal thickness through the Neoarchean–Paleoproterozoic, we posit the onset of an intraplate rifting consistent with the anomalous low‐δ18O magmatism. Continental rifting of Archean cratons/supercratons plausibly hints at the formation of rifts driving subduction initiation as the veritable onset of the Paleoproterozoic supercontinent cycle.
High‐Temperature Deformation of Enstatite‐Olivine Aggregates
Authors: Bystricky M. , Lawlis J. , Mackwell S. , Heidelbach F.
Published: March 2024

Synthesized polycrystalline samples composed of enstatite and olivine with different volumetric ratios were deformed in compression under anhydrous conditions in a Paterson gas‐medium apparatus at 1150–1300°C, an oxygen fugacity buffered at Ni/NiO, and confining pressures of 300 or 450 MPa (protoenstatite or orthoenstatite fields). Mechanical data suggest a transition from diffusion to dislocation creep with increasing differential stress for all compositions. Microstructural analyses by optical and scanning electron microscopy reveal well‐mixed aggregates and homogeneous deformation. Crystallographic preferred orientations measured by electron backscatter diffraction are consistent with activation of the slip systems (010)[100] and (010)[001] for olivine and (100)[001] and (010)[001] for enstatite, as expected at these conditions. Nonlinear least‐squares fitting to the full data set from each experiment allowed the determination of dislocation creep flow laws for the different mixtures. The stress exponent is 3.5 for all compositions, and the apparent activation energies increase slightly as a function of enstatite volume fraction. Within the limits of experimental uncertainties, all two‐phase aggregates have strengths that lie between the uniform strain rate (Taylor) and the uniform stress (Sachs) bounds calculated using the dislocation creep flow laws for olivine and enstatite. Calculation of the Taylor and Sachs bounds at strain rate and temperature conditions expected in nature (but not extrapolating in pressure) indicates that using the dislocation creep flow law for monomineralic olivine aggregates provides a good estimate of the viscosity of olivine‐orthopyroxene rocks deforming by dislocation creep in the deeper lithosphere and asthenosphere.
Stratocumulus Precipitation Properties Over the Southern Ocean Observed From Aircraft During the SOCRATES Campaign
Authors: Kang L. , Marchand R. T. , Wood R.
Published: March 2024

Precipitation plays an important role in cloud and aerosol processes over the Southern Ocean (SO). The main objective of this study is to characterize SO precipitation properties associated with SO stratocumulus clouds. We use data from the Southern Ocean Clouds Radiation Aerosol Transport Experimental Study (SOCRATES), and leverage observations from airborne radar, lidar, and in situ probes. We find that for the cold‐topped clouds (cloud‐top‐temperature <0°C), the phase of precipitation with reflectivity >0 dBZ is predominantly ice, while reflectivity < −10 dBZ is predominantly liquid. Liquid‐phase precipitation properties are retrieved where radar and lidar are zenith‐pointing. Power‐law relationships between reflectivity (Z) and rain rate (R) are developed, and the derived Z–R relationships show vertical dependence and sensitivity to the presence of droplets with diameters between 10 and 40 μm. Using derived Z–R relationships, a reflectivity‐velocity (ZV) retrieval method, and a radar‐lidar retrieval method, we derive rain rate and other precipitation properties. The retrieved rain rate from all three methods shows good agreement with in‐situ aircraft estimates, with rain rates typically being quite light (<0.1 mm hr−1). We examine the vertical distribution of precipitation properties, and find that rain rate, precipitation number concentration, and precipitation liquid water all decrease as one gets closer to the surface, while precipitation size and distribution width increases. We also examine how cloud base rain rate (RCB) depends on cloud depth (H) and aerosol concentration (Na) for particles with a diameter greater than 70 nm, and find that RCB is proportional to .
Multiple Evolution Modes of Megaripples in the Qaidam Basin and Implications for Ripple‐Like Aeolian Landforms on Mars
Authors: Li Chao , Zhang Zhi , Yu Lupeng , Chen Guoxiang , Yang Junhuai , Dong Zhibao
Published: March 2024

Aeolian landforms provide valuable insights into the planetary surface environment and its evolutionary history. In this study, the formation and evolution of megaripples in the Qaidam Basin and their relationship with the development environment are analyzed. By quantifying the wind environment, morphology, grain‐size distribution, sedimentary structure, and luminescence age of megaripples, we propose for the first time that there are multiple megaripple evolution modes. Investigation revealed that three evolution modes were responsible for forming megaripples in different equilibrium states: transient, stable, and metastable. Well‐sorted coarse sand grains accumulate on ridges and overlay poorly sorted fine sand grains to form transient megaripples. Stable megaripples have alternating sedimentary bedding of coarse and fine sand grains. Metastable megaripples have a secondary ripple formation on the surface. Throughout their formation, coarse and fine sand grains undergo regrouping. The response of coarse grains to the change in wind speed lags behind that of fine grains. This process controls the erosion and accumulation of megaripples and affects their size and sedimentary structures. The evolution mode, scale, and sedimentary structure of megaripples are influenced by the grain‐size range under the same wind conditions. The luminescence ages of the coarse‐grained megaripple sediments are less than 700 years. This study provides a fresh perspective on the coexistence of various sand ripples and transverse aeolian ridges found on Mars.
Large Wood Transport and Accumulation Near the Separation Zone of a Channel Confluence
Authors: Yuan Saiyu , Zheng Yuchen , Tang Hongwu , Chen Yihong , Xu Lei , Whittaker Colin , Gualtieri Carlo
Published: March 2024

Fallen trees enter the adjacent stream and are carried away downstream by the current. As the stream joins another one, the complex hydrodynamics near their confluence make the movement of wood hard to predict. These woods may accumulate near the confluence resulting in backwater and subsequent potential flooding. A laboratory study was conducted to investigate the movement and accumulation behavior of individual pieces of wood near the confluence. The characteristics of wood (i.e., the length, diameter, and density) and the hydraulic conditions (i.e., the discharge ratio and the release distance) were varied in this investigation. It was found that the wooden pieces released from the tributary got occasionally trapped in the flow separation zone of the confluence, whereupon they were mainly trapped by a clockwise vortex and continued to stay driven by a reverse cluster of currents within this zone. The accumulation probability of wood was mainly related to its length, the discharge ratio and the release distance. The effect of wood diameter and density within the tested parameters was negligible. The probability increased with an increase in the discharge ratio as well as a decrease in the release distance. The longer pieces had a higher probability of being trapped, whereas for those exceeding some critical value, the probability was nearly the same, or dropped sharply. A generalized model for wood accumulation near the confluence was developed for practical application. These findings carry significant implications for river management, particularly in preventing the risk of flooding caused by wood blockage.
Comparing body composition between the sweet-liking phenotypes: experimental data, systematic review and individual participant data meta-analysis
Authors: Armitage Rhiannon Mae , Iatridi Vasiliki , Sladekova Martina , Yeomans Martin Richard
Published: March 2024

Background

Legislation aimed at reducing sugar intake assumes that sweet-liking drives overconsumption. However, evidence that a greater liking for sweet taste is associated with unhealthier body size is mixed and complicated by relatively small samples, an overreliance on body mass index (BMI) and lack of classification using sweet-liking phenotypes.

Methods

We first examined body size data in two larger samples with sweet-liking phenotyping: extreme sweet-likers, moderate sweet-likers and sweet-dislikers. Adults (18-34yrs), attended a two-session lab-based experiment involving phenotyping for sweet-liking status and a bioelectrical impedance body composition measurement (Experiment One: N = 200; Experiment Two: N = 314). Secondly, we conducted an individual participant data (IPD) meta-analysis: systematic searches across four databases identified 5736 potential articles. Of these, 53 papers met our search criteria: a taste assessment that measured liking using sucrose (>13.7% w/v), which allowed sweet-liking phenotyping and included either BMI, body fat percentage (BF%), fat-free mass (FFM) or waist-circumference.

Results

A significant effect of sweet-liking phenotype on FFM was found in both Experiment One and Two, with extreme sweet-likers having significantly higher FFM than sweet-dislikers. In Experiment One, sweet-dislikers had a significantly higher BF% than extreme sweet-likers and moderate sweet-likers. However, as these data are from one research group in a young, predominantly westernised population, and the results did not perfectly replicate, we conducted the IPD meta-analyses to further clarify the findings. Robust one-stage IPD meta-analyses of 15 studies controlling for sex revealed no significant differences in BF% (n = 1836) or waist-circumference (n = 706). For BMI (n = 2368), moderate sweet-likers had slightly lower BMI than extreme sweet-likers, who had the highest overall BMI. Most interestingly, for FFM (n = 768), moderate sweet-likers and sweet-dislikers showed significantly lower FFM than extreme sweet-likers.

Conclusion

The higher BMI often seen in sweet-likers may be due to a larger FFM and questions the simple model where sweet liking alone is a risk factor for obesity.
High-power, electrically-driven continuous-wave 1.55-μm Si-based multi-quantum well lasers with a wide operating temperature range grown on wafer-scale InP-on-Si (100) heterogeneous substrate
Authors: Sun Jialiang , Lin Jiajie , Zhou Min , Zhang Jianjun , Liu Huiyun , You Tiangui , Ou Xin
Published: March 2024

A reliable, efficient and electrically-pumped Si-based laser is considered as the main challenge to achieve the integration of all key building blocks with silicon photonics. Despite the impressive advances that have been made in developing 1.3-μm Si-based quantum dot (QD) lasers, extending the wavelength window to the widely used 1.55-μm telecommunication region remains difficult. In this study, we develop a novel photonic integration method of epitaxial growth of III-V on a wafer-scale InP-on-Si (100) (InPOS) heterogeneous substrate fabricated by the ion-cutting technique to realize integrated lasers on Si substrate. This ion-cutting plus epitaxial growth approach decouples the correlated root causes of many detrimental dislocations during heteroepitaxial growth, namely lattice and domain mismatches. Using this approach, we achieved state-of-the-art performance of the electrically-pumped, continuous-wave (CW) 1.55-µm Si-based laser with a room-temperature threshold current density of 0.65 kA/cm−2, and output power exceeding 155 mW per facet without facet coating in CW mode. CW lasing at 120 °C and pulsed lasing at over 130 °C were achieved. This generic approach is also applied to other material systems to provide better performance and more functionalities for photonics and microelectronics.
Laser-upgraded coal tar for smart pavements in road and bridge monitoring applications
Authors: Huang Jincai , Zhang Man , He Haoyun , Li Qingang , Zhao Yixin , Tan Qiulin , Zang Xining
Published: March 2024

The implementation of an intelligent road network system requires many sensors for acquiring data from roads, bridges, and vehicles, thereby enabling comprehensive monitoring and regulation of road networks. Given this large number of required sensors, the sensors must be cost-effective, dependable, and environmentally friendly. Here, we show a laser upgrading strategy for coal tar, a low-value byproduct of coal distillation, to manufacture flexible strain-gauge sensors with maximum gauge factors of 15.20 and 254.17 for tension and compression respectively. Furthermore, we completely designed the supporting processes of sensor placement, data acquisition, processing, wireless communication, and information decoding to demonstrate the application of our sensors in traffic and bridge vibration monitoring. Our novel strategy of using lasers to upgrade coal tar for use as a sensor not only achieves the goal of turning waste into a resource but also provides an approach to satisfy large-scale application requirements for enabling intelligent road networks.
A first-in-class TIMM44 blocker inhibits bladder cancer cell growth
Authors: Zhang Lifeng , Shi Xiaokai , Zhang Lei , Mi Yuanyuan , Zuo Li , Gao Shenglin
Published: March 2024

Mitochondria play a multifaceted role in supporting bladder cancer progression. Translocase of inner mitochondrial membrane 44 (TIMM44) is essential for maintaining function and integrity of mitochondria. We here tested the potential effect of MB-10 (MitoBloCK-10), a first-in-class TIMM44 blocker, against bladder cancer cells. TIMM44 mRNA and protein expression is significantly elevated in both human bladder cancer tissues and cells. In both patient-derived primary bladder cancer cells and immortalized (T24) cell line, MB-10 exerted potent anti-cancer activity and inhibited cell viability, proliferation and motility. The TIMM44 blocker induced apoptosis and cell cycle arrest in bladder cancer cells, but failed to provoke cytotoxicity in primary bladder epithelial cells. MB-10 disrupted mitochondrial functions in bladder cancer cells, causing mitochondrial depolarization, oxidative stress and ATP reduction. Whereas exogenously-added ATP and the antioxidant N-Acetyl Cysteine mitigated MB-10-induced cytotoxicity of bladder cancer cells. Genetic depletion of TIMM44 through CRISPR-Cas9 method also induced robust anti-bladder cancer cell activity and MB-10 had no effect in TIMM44-depleted cancer cells. Contrarily, ectopic overexpression of TIMM44 using a lentiviral construct augmented proliferation and motility of primary bladder cancer cells. TIMM44 is important for Akt-mammalian target of rapamycin (mTOR) activation. In primary bladder cancer cells, Akt-S6K1 phosphorylation was decreased by MB-10 treatment or TIMM44 depletion, but enhanced after ectopic TIMM44 overexpression. In vivo, intraperitoneal injection of MB-10 impeded bladder cancer xenograft growth in nude mice. Oxidative stress, ATP reduction, Akt-S6K1 inhibition and apoptosis were detected in MB-10-treated xenograft tissues. Moreover, genetic depletion of TIMM44 also arrested bladder cancer xenograft growth in nude mice, leading to oxidative stress, ATP reduction and Akt-S6K1 inhibition in xenograft tissues. Together, targeting overexpressed TIMM44 by MB-10 significantly inhibits bladder cancer cell growth in vitro and in vivo.
USP22 regulates APL differentiation via PML-RARα stabilization and IFN repression
Authors: Kowald Lisa , Roedig Jens , Karlowitz Rebekka , Wagner Kristina , Smith Sonja , Juretschke Thomas , Beli Petra , Müller Stefan , van Wijk Sjoerd J. L.
Published: March 2024

Ubiquitin-specific peptidase 22 (USP22) is a deubiquitinating enzyme (DUB) that underlies tumorigenicity, proliferation, cell death and differentiation through deubiquitination of histone and non-histone targets. Ubiquitination determines stability, localization and functions of cell fate proteins and controls cell-protective signaling pathways to surveil cell cycle progression. In a variety of carcinomas, lymphomas and leukemias, ubiquitination regulates the tumor-suppressive functions of the promyelocytic leukemia protein (PML), but PML-specific DUBs, DUB-controlled PML ubiquitin sites and the functional consequences of PML (de)ubiquitination remain unclear. Here, we identify USP22 as regulator of PML and the oncogenic acute promyelocytic leukemia (APL) fusion PML-RARα protein stability and identify a destabilizing role of PML residue K394. Additionally, loss of USP22 upregulates interferon (IFN) and IFN-stimulated gene (ISG) expression in APL and induces PML-RARα stabilization and a potentiation of the cell-autonomous sensitivity towards all-trans retinoic acid (ATRA)-mediated differentiation. Our findings imply USP22-dependent surveillance of PML-RARα stability and IFN signaling as important regulator of APL pathogenesis, with implications for viral mimicry, differentiation and cell fate regulation in other leukemia subtypes.
Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Authors: Liu Jingtao , Tan Xiaomei , Li Li , Cao Liying , Zhou Yan , Li He , Peng Ting
Published: March 2024

Nucleolar protein 12 (NOL12), one of the nucleolar proteins which are primarily expressed in the nucleolus and play key roles in RNA metabolism, cell proliferation, cell cycle, and cell survival, is widely expressed in various species and multiple organs. Although it has been reported that the mRNA of Drosophila NOL12 homolog viriato is expressed in the eyes of Drosophila, the protein expression of NOL12 in mammalian eyes remains to be elucidated. In this study, we showed through immunohistochemistry that NOL12 was present in the rat retina, with predominant distribution in the cytoplasm of the retinal neuronal cells. In the human retinoblastoma cell line WERI-Rb1, we found that altering NOL12 expression led to a change in WERI-Rb1 cell viability. Knocking down NOL12 expression decreased cell viability. In contrast, overexpressing NOL12 increased cell viability. Furthermore, increasing NOL12 expression inhibited ultraviolet (UV)-induced apoptosis. These findings demonstrated that NOL12 may play an important protective role in retinal cells. In the WERI-Rb1 cells exposed to UV irradiation, we detected that NOL12 was degraded, but this degradation could be attenuated by a pan-Caspase inhibitor. Notably, the inhibitory effect of NOL12 against UV-induced apoptosis could be restrained by increasing the expression of ATR serine/threonine kinase (ATR), a kinase that, when activated by severe DNA damage, can result in apoptosis. We also found that upregulating NOL12 inhibited the activation of ATR caused by UV irradiation. Additionally, inhibiting ATR activity reduced apoptosis resulting from both silencing NOL12 expression and UV exposure. Thus, NOL12 may protect against UV irradiation-induced retinal damage by inhibiting ATR activity.
A second hotspot for pathogenic exon-skipping variants in CDC45
Authors: Schoch Kelly , Ruegg Mischa S. G. , Fellows Bridget J. , Cao Joseph , Uhrig Sabine , Einsele-Scholz Stephanie , Biskup Saskia , Hawarden Samuel R. A. , Salpietro Vincenzo , Capra Valeria ,   Undiagnosed Diseases Network, Brown Chris M. , Accogli Andrea , Shashi Vandana , Bicknell Louise S.
Published: March 2024

Biallelic pathogenic variants in CDC45 are associated with Meier-Gorlin syndrome with craniosynostosis (MGORS type 7), which also includes short stature and absent/hypoplastic patellae. Identified variants act through a hypomorphic loss of function mechanism, to reduce CDC45 activity and impact DNA replication initiation. In addition to missense and premature termination variants, several pathogenic synonymous variants have been identified, most of which cause increased exon skipping of exon 4, which encodes an essential part of the RecJ-orthologue’s DHH domain. Here we have identified a second cohort of families segregating CDC45 variants, where patients have craniosynostosis and a reduction in height, alongside common facial dysmorphisms, including thin eyebrows, consistent with MGORS7. Skipping of exon 15 is a consequence of two different variants, including a shared synonymous variant that is enriched in individuals of East Asian ancestry, while other variants in trans are predicted to alter key intramolecular interactions in α/β domain II, or cause retention of an intron within the 3ʹUTR. Our cohort and functional data confirm exon skipping is a relatively common pathogenic mechanism in CDC45, and highlights the need for alternative splicing events, such as exon skipping, to be especially considered for variants initially predicted to be less likely to cause the phenotype, particularly synonymous variants.
Understanding the infection severity and epidemiological characteristics of mpox in the UK
Authors: Ward Thomas , Overton Christopher E. , Paton Robert S. , Christie Rachel , Cumming Fergus , Fyles Martyn
Published: March 2024

In May 2022, individuals infected with the monkeypox virus were detected in the UK without clear travel links to endemic areas. Understanding the clinical characteristics and infection severity of mpox is necessary for effective public health policy. The study period of this paper, from the 1st June 2022 to 30th September 2022, included 3,375 individuals that tested positive for the monkeypox virus. The posterior mean times from infection to hospital admission and length of hospital stay were 14.89 days (95% Credible Intervals (CrI): 13.60, 16.32) and 7.07 days (95% CrI: 6.07, 8.23), respectively. We estimated the modelled Infection Hospitalisation Risk to be 4.13% (95% CrI: 3.04, 5.02), compared to the overall sample Case Hospitalisation Risk (CHR) of 5.10% (95% CrI: 4.38, 5.86). The overall sample CHR was estimated to be 17.86% (95% CrI: 6.06, 33.11) for females and 4.99% (95% CrI: 4.27, 5.75) for males. A notable difference was observed between the CHRs that were estimated for each sex, which may be indicative of increased infection severity in females or a considerably lower infection ascertainment rate. It was estimated that 74.65% (95% CrI: 55.78, 86.85) of infections with the monkeypox virus in the UK were captured over the outbreak.
Mitochondrial metabolism sustains CD8+ T cell migration for an efficient infiltration into solid tumors
Authors: Simula Luca , Fumagalli Mattia , Vimeux Lene , Rajnpreht Irena , Icard Philippe , Birsen Gary , An Dongjie , Pendino Frédéric , Rouault Adrien , Bercovici Nadège , Damotte Diane , Lupo-Mansuet Audrey , Alifano Marco , Alves-Guerra Marie-Clotilde , Donnadieu Emmanuel
Published: March 2024

The ability of CD8+ T cells to infiltrate solid tumors and reach cancer cells is associated with improved patient survival and responses to immunotherapy. Thus, identifying the factors controlling T cell migration in tumors is critical, so that strategies to intervene on these targets can be developed. Although interstitial motility is a highly energy-demanding process, the metabolic requirements of CD8+ T cells migrating in a 3D environment remain unclear. Here, we demonstrate that the tricarboxylic acid (TCA) cycle is the main metabolic pathway sustaining human CD8+ T cell motility in 3D collagen gels and tumor slices while glycolysis plays a more minor role. Using pharmacological and genetic approaches, we report that CD8+ T cell migration depends on the mitochondrial oxidation of glucose and glutamine, but not fatty acids, and both ATP and ROS produced by mitochondria are required for T cells to migrate. Pharmacological interventions to increase mitochondrial activity improve CD8+ T cell intratumoral migration and CAR T cell recruitment into tumor islets leading to better control of tumor growth in human xenograft models. Our study highlights the rationale of targeting mitochondrial metabolism to enhance the migration and antitumor efficacy of CAR T cells in treating solid tumors.
Local orchestration of distributed functional patterns supporting loss and restoration of consciousness in the primate brain
Authors: Luppi Andrea I. , Uhrig Lynn , Tasserie Jordy , Signorelli Camilo M. , Stamatakis Emmanuel A. , Destexhe Alain , Jarraya Bechir , Cofre Rodrigo
Published: March 2024

A central challenge of neuroscience is to elucidate how brain function supports consciousness. Here, we combine the specificity of focal deep brain stimulation with fMRI coverage of the entire cortex, in awake and anaesthetised non-human primates. During propofol, sevoflurane, or ketamine anaesthesia, and subsequent restoration of responsiveness by electrical stimulation of the central thalamus, we investigate how loss of consciousness impacts distributed patterns of structure-function organisation across scales. We report that distributed brain activity under anaesthesia is increasingly constrained by brain structure across scales, coinciding with anaesthetic-induced collapse of multiple dimensions of hierarchical cortical organisation. These distributed signatures are observed across different anaesthetics, and they are reversed by electrical stimulation of the central thalamus, coinciding with recovery of behavioural markers of arousal. No such effects were observed upon stimulating the ventral lateral thalamus, demonstrating specificity. Overall, we identify consistent distributed signatures of consciousness that are orchestrated by specific thalamic nuclei.
Float-stacked graphene–PMMA laminate
Authors: Kim Seung-Il , Moon Ji-Yun , Hyeong Seok-Ki , Ghods Soheil , Kim Jin-Su , Choi Jun-Hui , Park Dong Seop , Bae Sukang , Cho Sung Ho , Lee Seoung-Ki , Lee Jae-Hyun
Published: March 2024

Semi-infinite single-atom-thick graphene is an ideal reinforcing material that can simultaneously improve the mechanical, electrical, and thermal properties of matrix. Here, we present a float-stacking strategy to accurately align the monolayer graphene reinforcement in polymer matrix. We float graphene-poly(methylmethacrylate) (PMMA) membrane (GPM) at the water–air interface, and wind-up layer-by-layer by roller. During the stacking process, the inherent water meniscus continuously induces web tension of the GPM, suppressing wrinkle and folding generation. Moreover, rolling-up and hot-rolling mill process above the glass transition temperature of PMMA induces conformal contact between each layer. This allows for pre-tension of the composite, maximizing its reinforcing efficiency. The number and spacing of the embedded graphene fillers are precisely controlled. Notably, we accurately align 100 layers of monolayer graphene in a PMMA matrix with the same intervals to achieve a specific strength of about 118.5 MPa g−1 cm3, which is higher than that of lightweight Al alloy, and a thermal conductivity of about 4.00 W m−1 K−1, which is increased by about 2,000 %, compared to the PMMA film.
Systematic review and meta-analysis for a Global Patient co-Owned Cloud (GPOC)
Authors: Lidströmer Niklas , Davids Joe , ElSharkawy Mohamed , Ashrafian Hutan , Herlenius Eric
Published: March 2024

Cloud-based personal health records increase globally. The GPOC series introduces the concept of a Global Patient co-Owned Cloud (GPOC) of personal health records. Here, we present the GPOC series’ Prospective Register of Systematic Reviews (PROSPERO) registered and Preferred Reporting Items Systematic and Meta-Analyses (PRISMA)-guided systematic review and meta-analysis. It examines cloud-based personal health records and factors such as data security, efficiency, privacy and cost-based measures. It is a meta-analysis of twelve relevant axes encompassing performance, cryptography and parameters based on efficiency (runtimes, key generation times), security (access policies, encryption, decryption) and cost (gas). This aims to generate a basis for further research, a GPOC sandbox model, and a possible construction of a global platform. This area lacks standard and shows marked heterogeneity. A consensus within this field would be beneficial to the development of a GPOC. A GPOC could spark the development and global dissemination of artificial intelligence in healthcare.
Sociobiome - Individual and neighborhood socioeconomic status influence the gut microbiome in a multi-ethnic population in the US
Authors: Kwak Soyoung , Usyk Mykhaylo , Beggs Dia , Choi Heesun , Ahdoot Dariush , Wu Feng , Maceda Lorraine , Li Huilin , Im Eun-Ok , Han Hae-Ra , Lee Eunjung , Wu Anna H. , Hayes Richard B. , Ahn Jiyoung
Published: March 2024

Lower socioeconomic status (SES) is related to increased incidence and mortality due to chronic diseases in adults. Association between SES variables and gut microbiome variation has been observed in adults at the population level, suggesting that biological mechanisms may underlie the SES associations; however, there is a need for larger studies that consider individual- and neighborhood-level measures of SES in racially diverse populations. In 825 participants from a multi-ethnic cohort, we investigated how SES shapes the gut microbiome. We determined the relationship of a range of individual- and neighborhood-level SES indicators with the gut microbiome. Individual education level and occupation were self-reported by questionnaire. Geocoding was applied to link participants’ addresses with neighborhood census tract socioeconomic indicators, including average income and social deprivation in the census tract. Gut microbiome was measured using 16SV4 region rRNA gene sequencing of stool samples. We compared α-diversity, β-diversity, and taxonomic and functional pathway abundance by SES. Lower SES was significantly associated with greater α-diversity and compositional differences among groups, as measured by β-diversity. Several taxa related to low SES were identified, especially an increasing abundance of Prevotella copri and Catenibacterium sp000437715, and decreasing abundance of Dysosmobacter welbionis in terms of their high log-fold change differences. In addition, nativity and race/ethnicity have emerged as ecosocial factors that also influence the gut microbiota. Together, these results showed that lower SES was strongly associated with compositional and taxonomic measures of the gut microbiome, and may contribute to shaping the gut microbiota.
X-ray scattering tensor tomography based finite element modelling of heterogeneous materials
Authors: Auenhammer Robert M. , Kim Jisoo , Oddy Carolyn , Mikkelsen Lars P. , Marone Federica , Stampanoni Marco , Asp Leif E.
Published: March 2024

Among micro-scale imaging technologies of materials, X-ray micro-computed tomography has evolved as most popular choice, even though it is restricted to limited field-of-views and long acquisition times. With recent progress in small-angle X-ray scattering these downsides of conventional absorption-based computed tomography have been overcome, allowing complete analysis of the micro-architecture for samples in the dimension of centimetres in a matter of minutes. These advances have been triggered through improved X-ray optical elements and acquisition methods. However, it has not yet been shown how to effectively transfer this small-angle X-ray scattering data into a numerical model capable of accurately predicting the actual material properties. Here, a method is presented to numerically predict mechanical properties of a carbon fibre-reinforced polymer based on imaging data with a voxel-size of 100 μm corresponding to approximately fifteen times the fibre diameter. This extremely low resolution requires a completely new way of constructing the material’s constitutive law based on the fibre orientation, the X-ray scattering anisotropy, and the X-ray scattering intensity. The proposed method combining the advances in X-ray imaging and the presented material model opens for an accurate tensile modulus prediction for volumes of interest between three to six orders of magnitude larger than those conventional carbon fibre orientation image-based models can cover.
Artificial neural networks optimize the establishment of a Brazilian germplasm core collection of winter squash (Cucurbita moschata D.)
Authors: Gomes Ronaldo Silva , Machado Júnior Ronaldo , de Almeida Cleverson Freitas , de Oliveira Rebeca Lourenço , Nascimento Moysés , Nardino Maicon , do Nascimento Wellington Ferreira , da Silva Derly José Henriques
Published: March 2024

With widespread cultivation, Cucurbita moschata stands out for the carotenoid content of its fruits such as β and α-carotene, components with pronounced provitamin A function and antioxidant activity. C. moschata seed oil has a high monounsaturated fatty acid content and vitamin E, constituting a lipid source of high chemical–nutritional quality. The present study evaluates the agronomic and chemical–nutritional aspects of 91 accessions of C. moschata kept at the BGH-UFV and propose the establishment of a core collection based on multivariate approaches and on the implementation of Artificial Neural Networks (ANNs). ANNs was more efficient in identifying similarity patterns and in organizing the distance between the genotypes in the groups. The averages and variances of traits in the CC formed using a 15% sampling of accessions, were closer to those of the complete collection, particularly for accumulated degree days for flowering, the mass of seeds per fruit, and seed and oil productivity. Establishing the 15% CC, based on the broad characterization of this germplasm, will be crucial to optimize the evaluation and use of promising accessions from this collection in C. moschata breeding programs, especially for traits of high chemical–nutritional importance such as the carotenoid content and the fatty acid profile.