bluuebunny/crossref_metadata_2025 huggingface dataset:
Studies on guinea pig skin cell cultures. V. Co-culture of pigmented melanocytes and albino keratinocytes, a model for the study of pigment transfer
Authors: Prunieras M , Moreno G , Dosso Y , Vinzens F.
Published: July 2023

Mixed cultures of melanocytes (M) and keratinocytes (K) are easily obtained from pigmented guinea pig ear skin. They are suitable for the study of pigment transfer from M to K. However, quantitation is difficult because many K are already loaded with pigment prior to cultivation. A technique is presented in which pigment-producing M are co-cultured with K of albino origin. Pigmented guinea pig ear skin is split with trypsin and basal cells including M are harvested. The cell suspension is treated with sodium citrate which prevents the attachment of K (but not of M) to the culture substrate. Ninety per cent pure M cultures are obtained. Five to seven days later, another basal cell suspension is prepared, this time from albino ear skin. This second suspension is seeded on top of the pigment-forming culture of M. The number of contacts between albino K and pigment-forming M increases as a direct function of time. Contrarily, the number of K which become pigmented increases until the fifth day of co-culture and decreases thereafter.
Aggravation of psoriasis and occurrence of psoriasiform cutaneous eruptions induced by practolol (Eraldin)
Authors: Sondergaard J , Wadskov S , Jensen HA , Mikkelsen HI.
Published: July 2023

Twelve patients with cutaneous and/or eye symptoms caused by practolol have been studied. The morphological appearance of the skin eruptions was psoriasiform in 5, vesiculo-papular in one, and exfoliating dermatitis was seen in 2 patients. Keratoconjunctivitis sicca was the only adverse reaction to practolol in one and was present in 3 additional patients. Practolol was found to aggravate existing psoriasis in 3 patients and render it extremely refractory to therapy.
Study of elastolytic activity Propionibacterium acnes and Staphylococcus epidermis in acne vulgaris and in normal skin
Authors: Dick GF , Ashe BM , Rodgers EG , Diercks RC , Goltz RW.
Published: July 2023

Histopathological sections of anetoderma-like scars from 10 patients with acne vulgaris showed a selective absence of elastic fibers around pilosebaceous follicles. This finding is similar to the histologic changes of "perifollicular elastolysis" reported by Varadi. Bacteria isolated by anaerobic and aerobic cultures of swabs of the skin surface and pus of these 10 patients, 12 others with active acne vulgaris and 8 normal subjects were studied with particular attention to Staphylococcus epidermis and Propionibacterium acnes. These organisms were analysed for production of an elastolytic enzyme which might play a role in the observed selective loss of elastic fibers. No elastolytic activity was produced by S. epidermidis or P. acnes isolated from any of these individuals. Thus, we cannot attribute the perifollicular loss of elastic fibers in acne scarring to an elastase produced by organisms. The observed absence of elastic fibers might result from tissue necrosis produced by leukocytes during the inflammatory phase, followed by collagenous scar formation without regeneration of elastic fibers.
Comparison of desoximetasone and hydrocortisone butyrate in psoriasis
Authors: Zachariae H.
Published: July 2023

Thirty psoriatics were treated for 2 weeks on a double-blind controlled basis with desoximetasone (0.25%) and with hydrocortisone butyrate (0.1%). It was a randomised left-right comparative trial. Thirteen out of 27 patients preferred desoximetasone, 3 patients preferred hydrocortisone butyrate. There was also a significantly better effect of desoximetasone as judged by the observer after the second week of treatment.
Syringocystadenoma papilliferum: light and electron microscopic studies
Authors: Niizuma K.
Published: July 2023

Light microscopic and electron microscopic studies of a lesion displaying a typical histopathological picture of syringocystadenoma papilliferum (S.P.) are reported. Light microscopic serial sections showed that the tumor parenchyma extended to the epithelium just above the intrafollicular sebaceous duct. The tumor thus presumably develops from the epithelial region corresponding to the intrafollicular duct of the apocrine sweat gland. Electron microscopy revealed a non-keratinized intracytoplasmic cavity and intercellular canaliculi apparently formed as a continuation of this cavity. Keratinized cells were absent from the glandular and duct epithelium and even from the superficial epithelial portion of acanthotic areas. Neither myoepithelial cells nor secretory granules were identified in areas showing tubular, glandular, or sinusoidal structures. It was concluded that the tumor differentiates towards both the intrafollicular and intradermal duct of the embryonic apocrine sweat gland apparatus.
The inverted follicular keratosis. A surprising variant of seborrheic wart
Authors: Sim-Davis D , Marks R , Wilson-Jones E.
Published: July 2023

The pathological features of 29 lesions that had been diagnosed as inverted follicular keratoma have been reviewed. The clinical data suggest that these lesions occur predominantly on the face of middle-aged or elderly man. None were correctly diagnosed before removal. The main histological points noted were the presence of an invaginated type of architecture, foci of keratinization (squamous eddies) and areas of basaloid cells which were arranged haphazardly with mitotic figures (Borst-Jadassohn change). All the histological features seen are also observed in irritated or traumatized seborrheic warts and no evidence was found for the original suggestion that these lesions derive from hair follicles.
Recurrent bullous eruptions on the lower legs
Authors: Björnberg A , Mobacken H.
Published: July 2023

In 15 patients with venous or arterial insufficiency of the legs, bullous eruptions were observed on the lower legs. The bullae were subepidermally situated. Circulating antibodies against the basement membrane zone were not demonstrated in 2 cases investigated. This eruption seems to represent a clinical entity of unknown etiology.
Pathogenesis and staging of scleroderma
Authors: Winkelmann RK.
Published: July 2023

Scleroderma is reviewed as a complex disease of vascular, connective-tissue, and inflammatory reactions. If the scleroderma syndromes related to occupational, immunologic, inflammatory, metabolic, and genetic factors are recognized, then true progressive systemic scleroderma (PSS) appears to be a disease of vascular fibrosis with secondary inflammatory phases. The primary pathology appears to be a fibromucinous change of the vascular endothelium. The vessels also demonstrate altered reactivity to cold and to serotonin. Biopsy of the skin of the inflammatory or mesenchymal scleroderma may reveal (by positive direct immunofluorescence) deposition of globulins at the basement membrane zone. Evaluation of scleroderma is discussed and a system of clinical staging of PSS is proposed which relates the organ involvement to the prognosis.
Lichen simplex chronicus Vidal: comparative submicroscopic aspects of acanthotic disorders
Authors: Frithz A , Lagerholm B.
Published: July 2023

Electron microscopic analyses of lichen simplex chronicus Vidal (LSC) are reported. The submicroscopic organization is described. The frequent occurrence of collagen fibres directly juxtaposed to and contiguous with the lamina basalis seems to be a distinguishing feature of the LSC. Discontinuations in the lamina basalis are rarely indicated. A ubiquitous fragmentation and a certain paucity of tonofilamentous structure are present in cells preceding parakeratosis. There is an indubitable paucity of tonofilament-keratohyalin association. Mitochondria, endoplasmic reticulum and ribosomes are abundant. Odland bodies of type II are completely dominant. Parakeratosis and observed submicroscopically deficient or incomplete orthokeratosis are related to the numbers of defective Odland bodies. The keratinization of some acanthotic disorders is discussed.
Survival of Neisseria gonorrhoeae on surfaces
Authors: Elmros T.
Published: July 2023

The survival of Neisseria gonorrhoeae was investigated. It was found that gonococci could survive 24 hours in urethral secretion on a glass slide and on a towel at 22 degrees C, and 120 hours at 4 degrees C. A method was developed by which the survival of gonococci could be followed in vitro. With this method, gonococci grown in vitro were found to be protected by human serum, in contrast to gonococci resuspended in NaCl. The factors affecting the survival are discussed.
Cellular aspects of phototoxic reactions induced by kynurenic acid. I. Establishment of an experimental model utilizing in vitro cultivated cells
Authors: Wennersten G , Brunk U.
Published: July 2023

Kynurenic acid is an endogenously occurring tryptophan metabolite, which, under certain conditions, may accumulate in human tissues. In earlier work kynurenic acid showed a pronounced photohemolytic capacity, and conclusive evidence has been presented that the reaction is mediated by singlet oxygen. The aim of the present study was to gain further information about the cellular mechanisms involved in the photo-oxidative damage induced by kynurenic acid. This paper is mainly concerned with the establishment of a proper experimental model utilizing in vitro cultivated cells, but basal cytotoxic effects revealed by the use of cell growth curves, and light- and scanning electron microscopic examinations are also described.
Virus-like structures in lupus erythematosus discoides
Authors: Nagy E , Nagy IZ , Nagy-Vezekényi C.
Published: July 2023

Biopsy specimens from typical skin lesions of DLE patients were studied by means of electron microscopy. Virus-like structures were discovered in the cytoplasm of fibrocytes, endothelial cells and keratinocytes. After prolonged chloroquine treatment a decrease in the frequency and size of these structures was observed.
Neonatal sebaceous glands: fine structure of sebaceous and dendritic cells
Authors: Sato S , Hiraga K , Nishijima A , Hidano A.
Published: July 2023

Ultrastructural features of sebaceous glands in newborns were examined in adnexal polyp lesions of neonatal skin. Material for electron microscopy was obtained from the nipple areola of 10 Japanese newborn babies of both sexes, less than 8 days of age. The cell organization of the neonatal sebaceous acini consisted of undifferentiated, differentiating, and mature sebaceous cells, and the sequence of sebaceous transformation seemed to be consistent with that described in the postnatal acini. The sheet of continuous basal lamina covering the sebaceous acini was neither distorted nor convoluted. This configuration in the basal lamina revealed no convincing evidence for physiological involution of the neonatal sebaceous cells were identified at the outer periphery of the sebaceous glnads. Melanocytes in symbiosis with the sebaceous acini in concurrent presence of Langerhans cells.
Oral zinc sulphate therapy for acne vulgaris
Authors: Weismann K , Wadskov S , Sondergaard J.
Published: July 2023

A double-blind controlled clinical trial was performed to evaluate the effect of oral zinc sulphate, 0.6 g daily, on acne vulgaris. Twenty patients received zinc sulphate tablets and 19 were given placebo tablets. Thirteen of the zinc group and 12 of the placebo group received their medication throughout a 12-week period, while the remaining patients were treated for 4 or 8 weeks. In all patients the numbers or papular and pustular acne lesions on the face and the back were significantly reduced, while larger infiltrates remained practically unaltered during the trial, which was performed from March through May 1975. No statistically significant difference in the improvement of the groups was demonstrable. Pretreatment serum zinc values, which were normal in all patients, rose significantly in the zinc group as well as in the control group, but the increase in the former was significantly higher. The negative therapeutical results might be attributable to the limited number of patients or related to the zinc dosage. Furthermore, the results might have been influenced by the unexplained rise in serum zinc values in the control group. A possible weak beneficial effect of zinc might also have been camouflaged by the seasonal variation in the severity of acne which was noted in this study.
Treatment of alopecia areata with DNCB--an immunostimulation?
Authors: Frentz G , Eriksen K.
Published: July 2023

Ten patients with long-standing areate type alopecia totalis were sensitized with 1-chloro, 2, 4-dinitrobenzene (DNCB). Following sensitization they were painted once weekly on a 40 X 20 mm area of the vertex with DNCB in acetone, in concentrations adjusted to the allergic response. After 7 weeks, growth of hair was seen in the painted area in 3 patients and after 8 weeks all over the scalp in 3 other patients.
Oral zinc sulphate therapy for acne vulgaris
Authors: Michaelson G , Juhlin L , Vahlquist A.
Published: July 2023

Abstract is not available
Merthiolate allergy: a nationwide iatrogenic sensitization
Authors: Möller H.
Published: July 2023

Abstract is not available
Norwegian scabies developing during treatment with fluorinated steroid therapy
Authors: Millard LG.
Published: July 2023

A fit, normally intelligent young man developed Norwegian scabies after prolonged treatment with large quantities of clobetasol propionate ointment. The development of this overwhelming infestation from the normal eruption of scabies may be related to the local immunosuppressive effect of this potent topical steroid.
Periodic, functional disuse of limbs: report of a case
Authors: Barker DJ , Cunliffe WJ
Published: July 2023

The case is presented of a patient who developed periodic loss of function in individual limbs, and associated cyanosis and hyperkeratosis. Investigation excluded organic disease, and the condition responded to physiotherapy.
Circulating immune complexes in syphilis
Authors: Sølling J , Sølling K , Jakobsen KU , From E
Published: July 2023

13 patients with syphilis were investigated regarding the presence of circulating immune complexes by the methods of C 1q-binding-activity and anticomplementarity. Elevated C1q-binding-activity was demonstrated in 6 of 7 patients with secondary syphilis, a significantly greater incidence than among patients with primary syphilis and neurosyphilis. Anticomplementarity was demonstrated in five of seven patients with secondary syphilis and in two patients with neurosyphilis. Anticomplementarity was found in only one of four patients with primary syphilis. The presence of immune complexes may be of importance in the aetiology of some of the lesions of secondary and perhaps tertiary syphilis.
Zinc, liver cirrhosis and anorexia nervosa
Authors: Thomsen K
Published: July 2023

Abstract is not available
Inhibition of ultraviolet and phototoxic dermatitis in the mouse
Authors: Ljunggren B
Published: July 2023

The effect of inhibitors on the inflammatory oedema elicited by medium-wave ultraviolet radiation (UVB) and long-wave ultraviolet radiation (UVA) in combination with chlorpromazine has been studied in the mouse, by means of a quantitative technique. Inhibition of the UVB reaction was observed with indomethacin, acetylsalicylic acid and betamethasone valerate, whereas the latter compound only was effective in the phototoxic state. No inhibition was obtained with hydrocortisone, phenylbutazone, epsilon-aminocaproic acid, polyphloretin phosphate, clemastin, alpha-tocopherol or ascorbic acid. With indomethacin and betamethasone valerate there was no inhibition at high doses when the compound was administered before UVB irradiation. These results are in accordance with a proposed central role for the prostaglandins in UVB inflammation. It is suggested that the phototoxic reaction to chlorpromazine may not be due to mediator action but rather to the effect of toxic photoproducts.
Early fine structural changes in human epidermis following application of croton oil
Authors: Takigawa M , Komura J , Ofuji S
Published: July 2023

Early epidermal changes in human subjects following application of 10% croton oil in acetone were studied by electron microscopy. The prominent changes observed at 1 and 5 hours were degeneration of epidermal cells and intercellular edema in the basal and spinous layers. One type of degeneration was homogenization and aggregation of the tonofilaments of keratinocytes at 1 hour, which may explain the presence of dyskeratotic cells at 5 hours. Another type was of a cytolytic nature as evidenced by severe perinuclear edema of keratinocytes and destruction of Langerhans' cells with outpouring of Birbeck's granules in the intercellular spaces.
Micropapular sarcoidal facial eruption in a child: Gianotti-type perioral dermatitis
Authors: Georgouras K , Kocsard E
Published: July 2023

The case is described of a boy aged 13 years reported with a diffuse facial micropapular eruption which had a granulomatous histology. The diagnosis is consistent with perioral dermatitis of the Gianotti type (4, 5). It is postulated that bubble gum was the cause of the condition, the most likely ingredient being the essential oils in the gum, which may actually be selectively absorbed by the follicle to produce a perifollicular granulomatous reaction. In this article we have added yet another possible cause for perioral dermatitis.
The nature and role of epidermal regressive changes in the development of the psoriatic lesion
Authors: Neumann E
Published: July 2023

Damaged epidermal cells present in psoriatic lesions were investigated using histochemical and histomorphological methods including ultrathick sections. In early lesions these cells were found throughout the epidermis, being particularly numerous in the upper part of the latter and around the adnexa. In a later developmental stage the damage to the keratinocytes in the suprapapillary region was found to precede the damage to the basement membrane. These findings, together with the difference between the damaged keratinocytes lining the ascending side and the venous limb of the papilla respectively with regard to degree of severity of the damage, suggest that blood stasis is the cause of the regressive changes in the suprapapillary epidermis. The enzymatic activity of non-specific esterases and acid phosphatases was increased and that of succindehydrogenases and DPN-diaphorase was decreased in damage keratinocytes. The significance of these findings in relation to the genesis and development of psoriasis is discussed. The view is expressed that there is a causal relationship between damage to the epidermal cells and proliferation of epidermis.
Neutrophil granulocyte function in pustulosis palmoplantaris
Authors: Mattsby-Baltzer I , Mobacken H , Olling S , Rosén K
Published: July 2023

The phagocytic bactericidal capacity of circulating neutrophilic granulocytes was estimated in 22 patients with pustulosis palmoplantaris (PPP). The control group comprised 100 healthy individuals. Leukocytes from the patients had a statistically significant reduced capacity to kill Staph. aureus in autologous as well as in AB serum. Leukocyte killing of E. coli was not reduced. Serum levels of total hemolytic complement, C3, C4 and neutrophil myeloperoxidase activity were normal. The findings suggest an intrinsic or cellular defect of circulating neutrophil granulocytes in patients with PPP.
Pulsating fixed drug eruption due to tetracycline
Authors: Parish LC , Witkowski JA
Published: July 2023

A 29-year-old woman developed a pulsating, fixed drug eruption following a year's course of both oral and parenteral administration of tetracycline for pharyngitis. Patch testing of tetracycline powder under occlusion at the site confirmed the diagnosis.
Lupus erythematosus hypertrophicus: a case report
Authors: Howitz J , Ullman S
Published: July 2023

Abstract is not available
Plantar lesions of lichen sclerosus et atrophicus accompanied by erythermalgia
Authors: Hammar H
Published: July 2023

Abstract is not available
Acral arteriovenous tumor
Authors: Persson JR
Published: July 2023

Abstract is not available
Hormonal analysis and delayed hypersensitivity reactions in identical twins with severe acne
Authors: Palatsi R , Oikarinen A.
Published: July 2023

Identical twins aged 17 and another pair aged 21 are described. One pair had febrile ulcerative conglobate acne; the other, cystic acne. The location of acne, the type of the lesions and the course of the disease were very similar in the two twins of each pair. The testosterone levels of the 17-year-old pair varied and their acne was in the active stage, while the 21-year-old pair had high testosterone levels and their acne was abating. The 17-year-old pair had negative Mantoux reactions and they reacted negatively to DNCB sensitization. The authors suggest that acne skin may have a certain genetically determined local factor, e.g. hormone receptor, which gives rise to acne in a certain hormonal situation.
Chronic bullous dermatosis of childhood
Authors: Del Forno C , Giannetti A , Orecchia G.
Published: July 2023

A case of chronic bullous dermatosis of childhood in a 3-year-old boy is described. Immunoflourescence tests were negative and biopsy of the jejunal mucosa showed marked villous atrophy. The dermatosis was brought under control by a combination of diaminodiphenylsulphone and systemic steroids. The relationship with other bullous eruptions of childhood such as dermatitis herpetiformis and bullous pemphigoid is discussed.
Membrane damage caused by 8-MOP and UVA-treatment of cultivated cells
Authors: Wennersten G.
Published: July 2023

Treatment of red blood cells with a combination of 8-MOP and UVA did not cause any significant hemolysis under the experimental conditions used, and the deuterium test for identification of singlet oxygen mediated reactions was negative. However, similar experiments performed on in vitro cultivated human glia cells caused conspicuous surface membrane alterations, as revealed by scanning electron microscopy. The alterations were seen at higher concentration levels than was expected and might be secondary to earlier appearing intracellular events seen at lower concentrations. The clinical significance of membrane alterations for the results obtained with PUVA therapy require further investigation.
Synchronous balding of scalp and hair-bearing grafts of scalp transplanted to the skin of the arm in male pattern baldness
Authors: Nordström RE.
Published: July 2023

Abstract is not available.
Light and electron microscopic aspects of pemphigus herpetiformis (eosinophilic spongiosis) in comparison with other acantholytic disorders
Authors: Lagerholm B , Frithz A , Borglund E.
Published: July 2023

Histopathological and electron microscopic analyses of pemphigus herpetiformis in a 64-year old woman with a previously operated atoxic goitre and suffering from a nephrotic syndrome and a chronic non-aggressive hepatitis revealed acantholysis preceded by eosinophilic spongiosis. Light microscopically the acantholysis is characterized by the occurrence of necrobiotic epidermal cells. No dyskeratotic cells are found. The mechanism of acantholysis depends on an exuberant elongation of desmosomes, leading to their disruption, spongiotic acantholysis. A conspicuous convolution of specific tight intercellular spaces is observed. Intracytoplasmatic occurrence of such tight intercellular spaces is seen in sections. Pseudomyeloid bodies of various types seem to be of significance. Particles closely similar to pox viruses are observed. The acantholytic process is compared with other acantholytic disorders in which the above-mentioned findings were not observed.
Immunobiological aspects of Clq in sera of patients with cutaneous vasculitis and collagen diseases
Authors: Sano S , Shinkai H , Yonemasu K.
Published: July 2023

Clq was comparatively quantified with CH50 or C3 in sera of patients with various types of cutaneous vasculitis and collagen diseases. The following results were found: 1) Elevated levels of Clq were seen much more frequently in cutaneous vasculitis and PSS. 2) No significant correlations were found between Clq and CH50 or C3, except for a moderate rank correlation between Clq and C3 in SLE. 3) The amount of hydroxyproline in serum (collagen-like protein) is nearly identical with the calculated value of that present in Clq.
Treatment of generalized scleroderma: updated results
Authors: Asboe-Hansen G.
Published: July 2023

Long-term treatment of patients with generalized progressive scleroderma by means of inhibitors of connective-tissue biosynthesis brings about total or subtotal regression of dermal sclerosis in 40.8%, partial regression in 33.1%, arrest of progression without regression in 14.8%, while in 11.3% it had no effect whatsoever. The drugs used were D-penicillamine, benzyl-penicillin-diethyl-aminoethylesterhydro-iodide, glutamine, hydralazine, chlorpromazine, L-dopa, diphenylhydantoin, and corticosteroids. Disease activity before, during and after treatment was indicated by the urinary fractions of high-molecular hydroxyproline and hydroxylysine containing peptides and of uronic acid, break-down products of collagen and acid glycosaminoglycans of connective-tissue ground substance. The prospects were better for young patients than for old, for those with a short history than for the longstanding disease cases, and for those having a large total dose than for those who had less. If left untreated, scleroderma progresses inexorably.
Severe skin pain after PUVA treatment
Authors: Tegner E.
Published: July 2023

Severe skin pain lasting one or two months occurred in 8 of 210 patients treated with PUVA. The pain started 4--8 weeks after the initial dose, mostly about one week after discontinuation of the treatment. It was a prickling, burning pain, usually coming in bouts and confined to limited areas "deep under the skin". In some respects the pain was related to itching, but the patients could easily distinguish between the two sensations. A variety of drugs was tried, but none had any noteworthy effect on this peculiar pain.
Cyclic AMP and psoriasis once more
Authors: Wadskov S , Kassis V , Søndergaard J.
Published: July 2023

Imbalanced cyclic nucleotides are implicated as an underlying pathogenetic mechanism in psoriasis, but conflicting data on the levels of cAMP in psoriatic tissue have been obtained by different laboratories. Using heat separation and a competitive protein binding cAMP assay, a noticeably decreased epidermal level of cAMP was detected in newly formed guttate lesions in 10 patients with psoriasis (involved: 4.32 +/- 1.06 pmol/mg dry weight mean +/- S.E.M.). uninvolved: 7.97 +/- 1.63 pmol/mg dry weight mean +/- S.E.M.). In proliferative rat skin, bidirectional alterations in cAMP levels are known to occur. On the basis of present observation, we assume that the conflicting data hitherto obtained in psoriatic tissue similarly may merely reflect the various levels of cAMP at different developmental stages.
Ultrastructural study of mechanobullous desquamative gingivitis: a case report
Authors: Kossard S , Lofgren RK , Rogers 3rd. RS
Published: July 2023

A 67-year-old female with localized bullous desquamative gingivitis is described. Ultrastructural studies of the perilesional area showed changes similar to those seen in cicatricial pemphigoid and epidermolysis bullosa acquisita.
Chequered localized pseudoxanthoma elasticum: a variety of Christensen's exogenous pseudoxanthoma elasticum?
Authors: Dupre A , Bonafe JL , Christol B.
Published: July 2023

We report a very curious case of a condition which has never been described before. Its features are comparable but not quite identical with those of Christensen's saltpetre-induced PXE which the author considers to be an exogenous variety of pseudoxanthoma elasticum. Since our case appeared spontaneously without any accidental episode we prefer to designate it under the name 'localized PXE'. The chequered appearance of the lesions seems to be the characteristic feature of the disease.
An in vitro study on the effect of benzoyl peroxide 20%, gentamicin and phenoxyethanol on Staphylococcus aureus, Pseudomonas aeruginosa and Proteus species
Authors: Cunliffe WJ , Holland KT.
Published: July 2023

Abstract is not available.
A study of Sézary cells in peripheral blood and skin lesions
Authors: Livden JK , Matre R , Haugen A , Bjerke JR , Bang G , Wesenberg F.
Published: July 2023

A patient with S√©zary syndrome is presented. By phase contrast and scanning electron microscopy S√©zary cells were demonstrated in peripheral blood and in skin tissue. Investigation of atypical mononuclear cells seen in the peripheral blood showed that these cells lack receptors for sheep erythrocytes. C3 and Fcgamma. Similarly, an examination of dermal mononuclear cell infiltrates showed that most of the cells lack receptors for sheep erythrocytes, C3 and Fcgamma.
Disseminated superficial "actinic" porokeratosis
Authors: Kariniemi AL , Kotovirta ML , Stubb S.
Published: July 2023

An 81-year-old Finnish female had a 10-month history of a very pruritic eruption. In the clinical examination porokeratosis was suspected and histologically verified with the typical cornoid lamellae. The eruption involved also the unexposed areas of the skin. The patient had always avoided sunshine because it made her feel uncomfortable. The patient's sister, too, had a solitary lesion of porokeratosis. The pathomechanism of DSAP is discussed.
Seasonal variations of pigmented naevi. Intercorrelations of clinical and histological variables with special reference to seasonal variation.
Authors: Larsen T E , Mogensen S B , Holme I
Published: July 2023

During 1984, junctional and compound naevi were registered significantly more often during the summer half-year (May-October) than the total number of naevi, which showed no seasonal variation. A series of 342 of these junctional and compound naevi have been the subject of a blind histological classification. Clinical information was obtained by a questionnaire mailed to the patients. The intercorrelations of 19 histological and 10 clinical variables were studied by chi 2-test. The seasonal variation of these variables was further studied by chi 2-test and by Hewitt's test. Patient's hair colour, eye colour and sex as well as mitoses and localization showed a significant correlation to season of the year. The trends of these findings, compared with the information about tumour duration, indicate a short-term latency effect of UV light on naevi which are excised during the summer half-year.
Histamine release from skin mast cells and basophils in patients with urticaria pigmentosa.
Authors: Nolte H , Søderberg U , Thestrup Pedersen K , Schiøtz P O
Published: July 2023

Histamine release from dispersed skin mast cells may be used for functional studies on the mast cell. However, technical difficulties have hampered such studies. In the present study a new fiberglass-based histamine assay was applied to previously described dispersion techniques, using excision biopsies from 7 patients with urticaria pigmentosa, 3 with psoriasis as well as 4 with urticaria. However, sufficient mast cell numbers for performing histamine release could only be obtained from patients with urticaria pigmentosa. The average mast cell yield was 935 +/- 470 cells (mean +/- SD) per mg wet weight of tissue. The skin mast cells from these patients responded with dose-dependent histamine release to anti-IgE, calcium ionophore A23187, and N-formyl-methionyl-leucyl-phenylalanine challenge without previous passive sensitization. The pattern of histamine release of mast cells and corresponding blood basophils did not indicate substantial differences between the two cell types.
Silicone gel in the treatment of keloids.,"/pubmed/1969213
Authors: Murdoch M E , Salisbury J A , Gibson J R
Published: July 2023

In 4 healthy subjects a skin area 10 mm in diameter was exposed to twice the minimal erythema dose of UVB. Subcutaneous blood flow (SBF) in the area was measured by the local 133-Xenon washout technique before and 8, 24, 48 and 72 h after induction of inflammation. Local skin temperature (TS) was monitored with electrical thermocouples. SBF gradually increased by 400% and peaked 24 to 48 h after induction of inflammation, while TS peaked after 8 h (+3 degrees C). The disparity in skin temperature and subcutaneous blood flow indicates that TS is not the governing factor in the increase in SBF. As release of inflammatory mediators from the cutis influencing the subcutis and a local effect of UVB on subcutis are unlikely, we suggest the existence of a cutaneous-subcutaneous vascular reflex mechanism as an explanation for the increased subcutaneous blood flow.
An electron microscopic study of oral lesions in erythema multiforme.
Authors: Ruokonen H , Hietanen J , Malmström M
Published: July 2023

The ultrastructure of oral lesions in 6 patients with erythema multiforme (EM) and of one apparently clinically healthy oral mucosa in one patient with recurrent EM during remission is described. Alterations were observed in epithelium, basal lamina and lamina propria. Both intercellular and intracellular oedema, intracellular vacuolization, decreased numbers of desmosomes, and also loss of cytoplasmic organelles and occasional nuclei were noted in the epithelium. Inflammatory cells--mainly lymphocytes--were found intra-epithelially. Several discontinuities together with some evidence of duplication of the basal lamina were seen in five of the six lesional mucosa specimens. The inflammatory infiltrate in the lamina propria consisted mainly of lymphocytes, although plasma cells, neutrophilic and eosinophilic leukocytes, macrophages and mast cells were also found. Some of the mast cells were partly degranulated. The apparently clinically healthy oral mucosa in the patient with EM in remission showed mild inflammatory changes. The changes observed in the lesional mucosa in EM are thus according to our study mostly non-specific inflammatory alterations and are not pathognomonic for EM.
Family study on Melkersson-Rosenthal syndrome. Some hereditary aspects of the disease and review of literature.
Authors: Meisel-Stosiek M , Hornstein O P , Stosiek N
Published: July 2023

The medical records of 73 unrelated patients with either complete or incomplete Melkersson-Rosenthal syndrome attending our department between 1967 and 1985 were analyzed, and 42 of them as well as 171 of their relatives were examined. Lingua plicata was seen in 10, and other features were detected in 6 of the 42 families. The limited frequency of signs characteristic for the syndrome in the relatives examined suggest a multifactorial origin including a genetic basis.
C3d,g in normal human epidermal basement membrane.
Authors: Basset-Seguin N , Yancey K B
Published: July 2023

No abstract available.
Dermatomyositis-like syndrome in X-linked hypogammaglobulinemia. Case-report and review of the literature.
Authors: Thyss A , El Baze P , Lefebvre J C , Schneider M , Ortonne J P
Published: July 2023

A case of dermatomyositis-like syndrome is described in a 19-year-old man with a history of Bruton's hypogammaglobulinemia. Although the patient had central-nervous-system manifestations (seizures), no echovirus was isolated in the cerebrospinal fluid, in contrast to previously reported cases. Data for our case and the 15 cases previously reported in the literature are reviewed. HLA typing of our patient revealed the presence of HLA B8 and DR3, which seems to play a major role in juvenile dermatomyositis.
Halo dermatitis around tumours.
Authors: Tegner E , Björnberg A , Jonsson N
Published: July 2023

Circular dermatitis around different benign or malignant lesions were examined in 19 patients. The morphological picture was the same whether it occurred around an acquired naevus cell naevus, a congenital naevus cell naevus, a seborrheic keratosis, a stucco-keratosis, a keloid, a benign lentigo, an insect bite, a basal cell carcinoma, or a squamous cell carcinoma. The central lesions were macroscopically unaffected by the surrounding inflammation.
Vitamin D metabolism in psoriasis before and after phototherapy.
Authors: Guilhou J J , Colette C , Monpoint S , Lancrenon E , Guillot B , Monnier L
Published: July 2023

Epidermis plays a major role in vitamin D synthesis and is a target tissue for 1,25 (OH)2 vitamin D, which could be involved in abnormal proliferation and differentiation of psoriatic keratinocytes. We investigated plasma calcium, phosphorus, alkaline phosphatases, parathyroid hormone, 25 (OH) D, 24,25 (OH)2 D and 1,25 (OH)2 D in 15 control subjects and 20 psoriatic patients before and after 3 weeks of phototherapy (UVB or PUVA). Before irradiation, all parameters were similar in psoriatics and controls, except for serum phosphorus (lower in psoriasis p less than 0.01). After phototherapy, P rose to normal values in psoriatic patients; 25 (OH) D and 24,25 (OH)2 D were dramatically increased by UVB (but not by PUVA) in psoriatic patients as well as in controls; 1,25 (OH)2 D was unmodified in controls but was significantly increased in psoriasis. Since 1,25 (OH)2 D has been reported to be an effective treatment for psoriasis, the UV-induced increase in 1,25 (OH)2 D could account for the beneficial effect of phototherapy in psoriasis.
Epidermal rupture is the initiating factor for the Koebner response in psoriasis.
Authors: Powles A V , Baker B S , Rutman A J , McFadden J P , Valdimarsson H , Fry L
Published: July 2023

The Koebner phenomenon was studied using low pressure suction and/or sellotape stripping. Each of 10 patients with psoriasis had 5 suction blisters induced and sellotape stripping. In each patient the roof of 4 of the 5 blisters was removed. In 6 of the 10 patients, psoriasis developed where the blister roof was removed, but not where the blister was left intact. However, only 3 of these 10 patients were also Koebner-positive at the tape-stripped site. Similarly, in a group of 37 psoriasis patients who were sellotape-stripped alone, only 8 (21.6%) were Koebner-positive at the tape-stripped site. In another experiment, 5 suction blisters were produced in each of 4 patients. The roof of 4 blisters was removed, one of which was occluded. Psoriasis developed in 3 of the 4 patients, but only where the blister roof had been removed and left unoccluded. These findings suggest that rupture of the epidermis can initiate the Koebner response, but that secondary dermal events are necessary for a psoriatic lesion to form.
Pseudoporphyria and sunbeds.
Authors: Stenberg A
Published: July 2023

Five cases of pseudoporphyria with recurrent blistering and skin fragility especially of the hands are described. Porphyrin studies were normal. The histopathological investigations in three of these cases showed subepidermal bullae consistent with porphyria cutanea tarda. All patients had considerable sunbed exposure before symptoms appeared. One patient was taking naproxen, a weak photosensitizer.
Herpes simplex virus antigens and inflammatory cells in oral lesions in recurrent erythema multiforme. Immunoperoxidase and autoradiographic studies.
Authors: Malmström M , Ruokonen H , Konttinen Y T , Bergroth V , Segerberg-Konttinen M , Hietanen J , Nordström D , Haapala M
Published: July 2023

Herpes simplex virus (HSV) antigens were sought in 15 biopsy specimens from both lesional mucosa and clinically healthy looking oral mucosa between attacks in patients with erythema multiforme (EM). Four of the eight biopsy specimens obtained from lesional EM mucosa stained positively with HSV-1-and/or HSV-2-specific antisera applied in direct immunoperoxidase staining. Of the 16 tissue specimens used as controls, two displayed positive staining with HSV-1 and/or HSV-2. Five of the seven biopsy specimens from macroscopically healthy oral mucosa obtained between attacks from patients with recurrent EM stained positively with HSV-1 and/or HSV-2. Of the six tissue specimens used as controls, three stained positively. Most of the local inflammatory mononuclear cells belonged to the T cell series, mainly to the CD-4 subset. A small proportion of the local T cells were blast transformed as assessed by CD-25 expression and [3H]thymidine incorporation. This, together with the findings showing a lower degree of activation in the biopsy from macroscopically healthy looking mucosa between attacks suggest an active role of the cell-mediated immune response in the genesis of oral lesions in EM. The persistence of HSV antigens, and the well-established role of HSV as a precipitating factor in recurrent EM, suggest that HSV may be involved, but since HSV seems to be present in other mucosal lesions as well as in clinically healthy mucosa, quite frequently an additional, hitherto unknown factor must be present in order that EM may occur.
Use of alternative medicine by patients with atopic dermatitis and psoriasis.
Authors: Jensen P
Published: July 2023

In a questionnaire study at a university hospital in Norway, 227 of 444 patients with atopic dermatitis (51.1%) and 215 of 506 patients with psoriasis (42.5%) reported previous or current use of one or more forms of alternative medicine. Homoeopathy, health food preparations and herbal remedies were used most. Use was related to disease duration, disease severity and--among the atopic dermatitis patients--the inefficacy of therapy prescribed by physicians, as judged by the patients. The use of alternative medicine is commonplace and should
Notalgia paresthetica--puzzling posterior pigmented pruritic patch. Report on two cases.
Authors: Marcusson J A , Lundh B , Sidén A , Persson A
Published: July 2023

No abstract available.
Adrenergic urticaria and adrenergic pruritus.
Authors: Haustein U F
Published: July 2023

We report here on 2 patients with adrenergic urticaria and adrenergic pruritus, respectively. The lesions and features developed during phases of stress and during the attacks were associated with an increase in the plasma concentrations of noradrenalin, adrenalin and prolactin. The dopamine plasma level was elevated only in the case of adrenergic urticaria. The symptoms could be reproduced by intradermal injection of adrenalin and noradrenalin and treated successfully with propanolol, a blocker of beta-adrenergic receptors. Adrenergic urticaria is a rare but distinct entity, which has to be separated from cholinergic urticaria. Adrenergic pruritus seems to be a minor variant of adrenergic urticaria.
EXPERIENCE IN THE DEVELOPMENT AND APPLICATION OF AN EDUCATIONAL APPLIED BOARD GAME IN PHARMACEUTICAL CHEMISTRY CLASSROOM
Authors:   Kalinkina O. V. Ryazan State Medical University, Buchanova U. N. , Chernykh I. V. ,   Ryazan State Medical University, Kopanitsa M. A. ,   Ryazan State Medical University, Povetko M. I. ,   Ryazan State Medical University, Kirichenko E. E. ,   Ryazan State Medical University, Kalinkina O. V. ,   Ryazan State Medical University
Published: July 2023

The article presents the experience of practical implementation of an applied board game for the study of pharmaceutical chemistry in the process of professional training at the Ryazan State Medical University. The prerequisites for the emergence of a gaming solution are described, which are primarily associated with changes in thinking, understanding and ability of the modern generation of students to work with various types of information. The characteristic features of the educational game as one of the types of interactive learning are given, and its main advantages are revealed: activation of students' thinking, increasing their overall activity in the classroom, independence; formation of the ability to work in a team, to respect the opinions of others, as well as, tolerance for different points of view. The paper notes an important cognitive advantage of using the game in the educational process, which is to increase the quality and speed of assimilation of the educational content by students. Attention is paid to the possibility of applied games to solve problems that are beyond the scope of gaming activity, the format being very popular in the modern world. The forms of the game implementation within the framework of full-time and distance learning are described, a brief description of the game itself is given, studies of students’ reaction to the game process are given and recommendations on the use of the game in the practice of education are described.
CLINICAL AND PSYCHOLOGICAL ASPECTS OF DRUG-RESISTANT EPILEPSY
Authors:   Ryazan State Medical University, Evdokimova O. V. , Elmi O. ,   Ryazan State Medical University, Zhadnov V. A. ,   Ryazan State Medical University, Yakovleva N. V. ,   Ryazan State Medical University
Published: July 2023

Drug-resistant epilepsy is determined by both clinical and subjective factors that are realized through psychological, behavioral and social mechanisms leading to low compliance, behaviors that help maintain a certain frequency of seizures, deformation of the motivational and emotional spheres of patients. The purpose of the study is to analyze the clinical, psychological characteristics of patients with drug-resistant epilepsy, to identify significant correlations between clinical and psychometric characteristics in patients with different course of epilepsy. The study included 60 patients diagnosed with epilepsy, divided into 2 groups: 30 patients with drug-resistant epilepsy and 30 patients with seizure remission. Patients underwent psychometric tests. Statistical differences were assessed using non-parametric statistics, Spearman's coefficient was used for correlation analysis, data at p < 0.05 were considered reliable. Clinical predictors of drug-resistant epilepsy were consistent with those described earlier in the literature: early onset of seizures, long duration of the disease and frequency of seizures, the prevalence of focal seizures, symptomatic structural variant of epilepsy. Negative correlations have been found between seizure frequency and levels of neuroticism and self-destructive behavior. Thus, high anxiety caused a more responsible attitude of patients to the treatment process, increasing their compliance. In patients with drug-resistant epilepsy, negative personality characteristics correlated with insufficiency of the inhibition process. The influence of neuroticism, alexithymia extended to a greater number of personality traits and behavior, indicating their systemic impact on the behavior of patients. The construction of models of behavior in conflict by patients with drug-resistant epilepsy reflected a tendency to compensate for personality traits through ineffective avoidance behavior, to demonstrate a lack of flexibility. Drug-resistant epilepsy is an important medical and social problem. The relationship of the disease with psychological and behavioral characteristics is close and complex, so such patients need clinical and psychological support to optimize the treatment process.
Peningkatan Kualitas Sumber Daya Manusia melalui Pelatihan Berwirausaha dalam Menumbuhkan Soft Skill pada Siswa SMK N 5 Sukoharjo
Authors: Utomo Chandra Wisnu , Puspita Dela , Insani Kharisma Aulia
Published: July 2023

Tujuan Pengabdian kepada masyarakat ini adalah untuk meningkatkan skill para siswa SMK N 5 Sukoharjo untuk lebih mengimplementasikan beberapa softskill dari jiwa wirausaha untuk dapat nantinya di implementasikan kedalam dunia kerja dan mampu meningkatkan kualitas dari sumbersaya manusia yang dimiliki oleh para siswa. Metode yang diberikan adalah pengarahan, presentasi, diskusi, studi kasus dan evaluasi akhir. Kegiatan ini bertujuan untuk memberikan pemahaman dan penguasaan soft skill untuk peningkatan kualitas sumber daya manusia. Peserta yang hadir dalam sosialisasi serta pendampingan ini sebanyak 50 siswa dari SMK N 5 Sukoharjo. Hasil dari kegiatan ini menyimpulkan bahwa kegiatan ini sangat membantu peserta dalam peningkatan berwirausaha dan pemahaman serta kesadaran mereka dalam peningkatan soft skil.