bluuebunny/crossref_metadata_2025 huggingface dataset:
From Inception to Implementation: Strategies for Setting Up Pulmonary Telerehabilitation
Authors: Santos Catarina Duarte , Rodrigues Fátima , Caneiras Cátia , Bárbara Cristina
Published: May 2022

Background

The emergence of innovative technology-enabled models of care is an opportunity to support more efficient ways of organizing and delivering healthcare services and improve the patient experience. Pulmonary telerehabilitation started as a promising area of research and became a strategic pandemic response to patients' decreased accessibility to rehabilitation care. Still, in the pre-COVID-19 era, we conducted a participatory study aiming to develop strategies for setting up pulmonary telerehabilitation as a person-centered digitally-enabled model of care.

Methods

We performed operational participatory research between June 2019 and March 2020 with the engagement of all stakeholders involved in the implementation of pulmonary telerehabilitation, including 14 people with Chronic Obstructive Pulmonary Disease. Patients were assessed subjectively and objectively pre and post a 3-month pulmonary rehabilitation program including exercise and education, which started in a face-to-face hospital setting during the first month and continued as a home-based, remotely supervised exercise training intervention.

Results

Five major groups of requirements targeted operational strategies for setting up pulmonary telerehabilitation: (1) pulmonary rehabilitation core principles, (2) quality and security standards, (3) technological functionality, (4) home environment appropriateness, and (5) telesetting skills. There was a statistical significance in the median change in the CAT score from 15.5 to 10.5 (p = 0.004) and in the PRAISE score from 49.5 to 53.0 (p = 0.006). Patients' mean levels of satisfaction regarding rehabilitation goals achievements were 88.1 ± 8.6% and the mean levels of satisfaction regarding the telerehabilitation experienced as a model of care were 95.4% ± 6.3%.

Conclusions

The success of telerehabilitation implementation was grounded on stakeholder engagement and targeted strategies for specific setup requirements, achieving patients' high satisfaction levels. Such operational experiences should be integrated into the redesigning of upgraded telerehabilitation programs as part of the solution to improve the effectiveness, accessibility, and resilience of health systems worldwide.
How Was Studied the Effect of Manual Wheelchair Configuration on Propulsion Biomechanics: A Systematic Review on Methodologies
Authors: Fritsch Capucine , Poulet Yoann , Bascou Joseph , Thoreux Patricia , Sauret Christophe
Published: May 2022

Background

For both sports and everyday use, finding the optimal manual wheelchair (MWC) configuration can improve a user's propulsion biomechanics. Many studies have already investigated the effect of changes in MWC configuration but comparing their results is challenging due to the differences in experimental methodologies between articles.

Purpose

The present systematic review aims at offering an in-depth analysis of the methodologies used to study the impact of MWC configuration on propulsion biomechanics, and ultimately providing the community with recommendations for future research.

Methods

The reviewing process followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) flowchart on two databases (Scopus and PubMed) in March 2022.

Results

Forty-five articles were included, and the results highlighted the multiplicity of methodologies regarding different experimental aspects, including propulsion environment, experimental task, or measurement systems, for example. More importantly, descriptions of MWC configurations and their modifications differed significantly between studies and led to a lack of critical information in many cases.

Discussion

Studying the effect of MWC configuration on propulsion requires recommendations that must be clarified: (1) the formalism chosen to describe MWC configuration (absolute or relative) should be consistent with the type of study conducted and should be documented enough to allow for switching to the other formalism; (2) the tested MWC characteristics and initial configuration, allowing the reproduction or comparison in future studies, should be properly reported; (3) the bias induced by the experimental situation on the measured data must be considered when drawing conclusions and therefore experimental conditions such as propulsion speed or the effect of the instrumentation should be reported.

Conclusion

Overall, future studies will need standardization to be able to follow the listed recommendations, both to describe MWC configuration and mechanical properties in a clear way and to choose the experimental conditions best suited to their objectives.
Environmental, Health, and Equity Co-benefits in Urban Climate Action Plans: A Descriptive Analysis for 27 C40 Member Cities
Authors: Johnson Lauren , Krisko Perrin , Malik Maha , O'Donnell Catherine , Pendleton Nick , Ahn Doyeon , Bizberg Andrea , Chafe Zoe A. , Kim Daniel , McCormick Sabrina , Naidoo Seneca , Anenberg Susan C.
Published: May 2022

Many actions to reduce greenhouse gases (GHGs) in cities have benefits for environmental quality, public health, and equity. These local and immediate “co-benefits” can include cleaner air, expanded green space, improved physical activity, and reduced noise. However, progress incorporating co-benefits assessments into climate mitigation planning has been limited. Here, we capitalized on the new availability of climate action plans (CAPs) from dozens of C40 cities to explore the stated role of environmental quality, health, and equity in urban GHG mitigation planning. Specifically, we qualitatively reviewed how four topics—equity, exposure to environmental risk factors, health effects of climate change, and health co-benefits of GHG mitigation—were addressed in CAPs from 27 C40 member cities. The cities span Africa, Asia, Australia, Europe, North America, and South America. We found that more references pertained to exposures (57% of all identified references across the four topics) than to equity (21%), health co-benefits of GHG mitigation (15%), or health effects of climate change (7%). On average across all cities, five exposure categories represented the majority of exposure references: green space (23% of total coded exposure references), disasters (20%), physical activity (24%), heat (16%), and air quality (12%). Approximately two-thirds of health effects and health co-benefits references noted a link with health generally, without specificity to disease or other health outcome. Our results indicate that while environmental quality is commonly considered in CAPs, health effects of climate change and health co-benefits of GHG mitigation are mentioned less frequently. Future work should further develop methods to qualitatively and quantitatively assess health consequences of action and inaction to reduce GHG emissions, using approaches that are appropriate for and accessible to multiple levels of governments.
Reduction of Spectral Radiance Reflectance During the Annular Solar Eclipse of 21 June 2020 Observed by EPIC
Authors: Wen Guoyong , Marshak Alexander , Herman Jay , Wu Dong
Published: May 2022

The annular solar eclipse on 21 June 2020 passed over desert areas (parts of Central and Eastern Africa, the southern Arabian Peninsula), partly cloudy regions (parts of South Asia and the Himalayas), and the mostly cloudy region in East Asia. Moving around the Earth-Sun Lagrange point 1 (L1), the Earth Polychromatic Imaging Camera (EPIC) instrument on the Deep Space Climate Observatory (DSCOVR) spacecraft captured three sets of images of the sunlit Earth during the eclipse, allowing us to study the impact of the solar eclipse on reflected solar radiation when the underlying surface and/or cloudy conditions in the Moon’s shadow are quite different. We analyzed EPIC images acquired during the 21 June 2020 and 21 August 2017 eclipses. We found that (1) EPIC-observed average spectral as well as spectrally averaged reflectance reductions of the entire sunlit Earth during the 21 June 2020 solar eclipse are distinctly different from those during the total solar eclipse of 21 August 2017; (2) the reduction of spectral reflectance depends strongly on underlying reflector properties, including the brightness, the area coverage of each reflector in the penumbra and the average distance to the center of the Moon’s shadow.
How Can Web Lessons Be Taught to Reduce Screen Fatigue, Motivational, and Concentration Problems in Different Disciplines?
Authors: Lepp Marina , Luik Piret , Tark Triin Mirjam
Published: May 2022

When conducting web lessons after transitioning to online learning due to the coronavirus, lecturers can base their work on experiences gained during the emergency situation and instructions prepared by the teaching support staff. However, students' perception of engagement strategies, screen fatigue, difficulties motivating themselves and problems with concentrating on web lessons should be also taken into account. The goal of this research is to find out how students rate the importance of engagement strategies in web lessons and how the ratings differ between disciplines. Also, the study aims to investigate how concentration difficulties, students' motivational problems and screen fatigue are connected to student engagement strategies in web lessons. To achieve that goal, 430 students of the Faculty of Sciences and Technology and the Faculty of Social Sciences at the University of Tartu were surveyed and multivariate analyses of variance and correlational analyses were conducted. Regarding student engagement strategies, “Use of slides” was found to be the most important by students. This was followed by “Explaining what and why is being studied,” “Sharing the teacher's screen,” and “Recalling what has been learned before”. The least important engagement strategies are “Presentation of article-based tasks,” “Second teacher answers questions in a chat,” and “Use of breakout rooms”. Comparing the faculties there was a statistically significant difference in the ratings given to five engagement strategies. The results showed that concentration difficulties were not related to the ratings of student engagement strategies. However, some positive correlations were found between screen fatigue and motivational difficulties, and the ratings of student engagement strategies, indicating that proper engagement strategies for conducting web lessons can be a way of influencing more students and better course design can support different needs.
Monomodality versus Combined Therapy in Optic Pathway Gliomas—20-Year Experience from a Singapore Children’s Hospital
Authors: Lim Jia Xu , Tan Enrica E.K. , Ng Lee Ping , Seow Wan Tew , Chang Kenneth T.E. , Wong Ru Xin , Looi Wen Shen , Low David C.Y. , Low Sharon Y.Y.
Published: May 2022

Introduction

The treatment of pediatric optic pathway gliomas (OPG) is challenging. At present, most centers provide individualized treatment to maximize progression free survival (PFS) and minimize morbidity. We aim to report our experience in the management of pediatric OPG, and investigate factors associated with an increased duration of remission after treatment.

Methods

This is a single-institution study approved by the hospital ethics board. A retrospective review of consecutive OPGs managed from 2000 to 2020 was performed. Patients were divided into those managed with monomodality treatment (MT) and those who received combined therapy (CT). MT included various forms of surgery, chemotherapy and radiotherapy given alone, while CT involves a combination of surgery and adjuvant chemotherapy and/or radiotherapy.

Results

Twenty-two patients were selected for this study. They had 40 treatment cycles; and a total follow up duration of 194.8 patient-years. Most of them were male (63.6%) and presented with visual deficits (72.7%). The mean age at initial presentation was 65 months and majority (86.4%) had their tumors arising directly from the optic chiasm, with 77.3% with hypothalamic extension. One patient had Neurofibromatosis type I (4.5%). The most common histological diagnosis was pilocytic astrocytoma (90.9%), followed by pilomyxoid astrocytoma (9.1%). The 5- and 10- year PFS were 46.2% and 36.4% respectively, while the 5- and 10-year OS were both 100%. When accounting for treatment type, there were 24 treatment cycles with MT (60.0%) and 16 CT (40.0%). After adjustment, treatments with MT were shown to have a shorter mean duration of remission (MT: 45 ± 49, CT: 84 ± 79 months; p = 0.007). Cox regression curve plotted after adjusting for patient’s age at treatment demonstrated a significantly longer PFS in the CT group (p = 0.037).

Conclusions

Our results suggest a significant survival benefit of CT over MT for affected patients due to the prolonged the duration of disease remission, for both primary and subsequent treatments. Nonetheless, we acknowledge that our study reflects the outcomes of treatment strategies that have evolved over time. We emphasize the need for collective efforts from a dedicated multidisciplinary team and international collaborations for better disease understanding.
Comparison of the Efficacy and Safety of Dexmedetomidine Administered in Two Different Modes Under Procedural Sedation and Analgesia in Plastic Surgery
Authors: Xia Weipeng , Wang Shanshan , Wei Lingxin , Deng Xiaoming , Yang Dong , Sui Jinghu , Liu Juhui
Published: May 2022

Background

Dexmedetomidine (DEX), a highly selective α2-adrenergic receptor agonist, is now widely used in procedural sedation and analgesia. This study was designed to observe and compare the efficacy and safety of DEX administered in two different modes.

Methods

In total, 100 patients were randomly divided into two groups to receive intravenous DEX 1 µg/kg over 15 min followed by 0.4–0.7 µg/kg/h infusion or DEX 1 µg/kg over 30 min followed by 0.4–0.7 µg/kg/h infusion. Heart rate (HR), mean arterial pressure (MAP), respiratory rate (RR), bispectral index (BIS), Ramsay Sedation Scores (RSS scores), the lowest respiratory rates (LRR), incidences of respiratory adverse events and frequencies of body movements were recorded. Recovery time, recall of intraoperative events, pain scores in PACU and satisfaction of patients and surgeons were assessed.

Results

The BIS at time points from 5 min after anesthesia to the end of surgery in the intervention group were significantly higher (p < 0.05). The RSS scores at time points from 5 min after anesthesia to immediately after induction with DEX were significantly higher in the intervention group (p < 0.05). The HR at time points from the beginning of surgery to 30 min after local anesthesia, the MAP at time points from 30 min after local anesthesia to the end of surgery, and the RR at time points from 5 min after anesthesia to the end of surgery were significantly higher in the intervention group (p < 0.05). Patients in the intervention group had higher LRR, lower incidences of respiratory adverse events, and shorter recovery time (p < 0.05).

Conclusions

Dexmedetomidine infused with a loading dose over 30 min had less impact on patients’ hemodynamics and respiration and could shorten the recovery time after anesthesia in procedural sedation and analgesia.

Clinical Trial Registration

ClinicalTrials.gov, identifier: ChiCTR1900027958.
Design and Development of a Disposable Superfine Catheter for Visual Examination of Bile Ducts and Related Animal Experiments
Authors: Hao Jin-Yong , Zhang Yao-Ping , Huang Xiao-Jun
Published: May 2022

Objective

To design and develop a disposable superfine catheter system for visual examination of bile and pancreatic ducts and predict its clinical application value.

Methods

The superfine triple-lumen catheter and miniature photography technology were used to design and produce a disposable superfine catheter for visual examination of bile and pancreatic ducts, and animal experiments were conducted to compare said catheter and SpyGlass™.

Results

The designed and developed disposable superfine catheter for visual examination of bile ducts with a diameter of 2.4 mm could enter the third-order and fourth-order bile ducts in the animal liver and also the gallbladder via the cystic duct for observation. The said catheter has a water injection rate of 0.8 mL/s, 0.16 megapixels, a resolution of 400 × 400, a depth of field of 0.3 to 20 mm, and a tilting up angle >90°.

Conclusion

The new disposable catheter for visual examination of bile ducts has a superfine diameter, easier operation, and clearer imaging, and is expected to have a higher clinical practical value.
Cytotoxicity of Medicinal Plant Species Used by Traditional Healers in Treating People Suffering From HIV/AIDS in Uganda
Authors: Anywar Godwin Upoki , Kakudidi Esezah , Oryem-Origa Hannington , Schubert Andreas , Jassoy Christian
Published: May 2022

Introduction: Many people living with HIV/AIDS (PLHIV) in Uganda widely use herbal medicines. However, their toxicity and safety have not been investigated. The use of these plants can potentially cause harmful effects to the health of patients. The purpose of this study was to determine the cytotoxicity of some commonly used medicinal plant species used by PLHIV.

Methods: The cytotoxicity of the plant extracts was determined with the AlamarBlue cell viability assay using the human glioblastoma cell line U87.CD4.CXCR4. The cells were treated with varying concentrations of extracts of Warburgia ugandensis, Erythrina abyssinica, Cryptolepis sanguinolenta, Albizia coriaria, Psorospermum febrifugium, Gymnosporia senegalensis, Zanthoxylum chalybeum, Securidaca longipendunculata, Vachellia hockii, Gardenia ternifolia, and Bridelia micrantha reconstituted with ethanol and dimethyl sulfoxide (DMSO). Using regression analysis, the half maximal cytotoxic concentration (CC50) of the plant extracts were calculated from exponential curve fits, since they provided the highest coefficient of determination, R2.

Results: The ethanol extracts of W. ugandensis (CC50 = 7.6 μg/ml) and A. coriaria (CC50 = 1.5 μg/ml) as well as the DMSO-reconstituted extracts of W. ugandensis (CC50 = 6.4 μg/ml) and A. coriria (CC50 = < 4 μg/ml) were highly cytotoxic. The cytotoxicity of W. ugandensis and A. coriaria compared well with the indigenous traditional knowledge of the toxic effects experienced when the plants were not used correctly. However, the cytotoxicity of most of the plant extracts (15/22) was low to moderate (CC50 = 21–200 μg/ml).

Conclusion: Most of the plant species tested in this study had low to moderate cytotoxicity against U87.CD4.CXCR4 cells, except W. ugandensis and A. coriria which were highly cytotoxic.
Working Dog Service, Harmful Agent Exposure and Decontamination
Authors: Jarrett Carla L. , Brathwaite Morgan , Gogal Robert M. , Holladay Steven D.
Published: May 2022

Working dogs are widely used by service professionals and the military for diverse roles that include sentry, patrol, messenger, tracking, search and rescue, law enforcement, apprehension, as well as explosives and narcotics detection. The expected tasks performed are in many ways determined by the breed, which is customarily a German Shepherd, Dutch Shepherd, Golden Retriever, Border Collie, Labrador Retriever, Beagle, or Belgium Malinois. Working dogs may be subject to injury from dangerous work environments or harmful agent exposure. Personal protective equipment (PPE) has been developed for such dogs, but may impede performance of duties or be poorly tolerated. Canine-specific field-use ready decontamination techniques and kits are therefore needed for use on working dogs that have encountered a harmful agent exposure. This report briefly reviews the development of the military working dog and examines personal protective equipment and decontamination techniques for working dogs after exposure to harmful biologic or chemical agents.
Novel Diagnostic Value of Driver Gene Transcription Signatures to Characterise Clear Cell Renal Cell Carcinoma, ccRCC
Authors: Ujfaludi Zsuzsanna , Kuthi Levente , Pankotai-Bodó Gabriella , Bankó Sarolta , Sükösd Farkas , Pankotai Tibor
Published: May 2022

Routine molecular tumour diagnostics are augmented by DNA-based qualitative and quantitative molecular techniques detecting mutations of DNA. However, in the past decade, it has been unravelled that the phenotype of cancer, as it’s an extremely complex disease, cannot be fully described and explained by single or multiple genetic variants affecting only the coding regions of the genes. Moreover, studying the manifestation of these somatic mutations and the altered transcription programming—driven by genomic rearrangements, dysregulation of DNA methylation and epigenetic landscape—standing behind the tumorigenesis and detecting these changes could provide a more detailed characterisation of the tumour phenotype. Consequently, novel comparative cancer diagnostic pipelines, including DNA- and RNA-based approaches, are needed for a global assessment of cancer patients. Here we report, that by monitoring the expression patterns of key tumour driver genes by qPCR, the normal and the tumorous samples can be separated into distinct categories. Furthermore, we also prove that by examining the transcription signatures of frequently affected genes at 3p25, 3p21 and 9p21.3 genomic regions, the ccRCC (clear cell renal cell carcinoma) and non-tumorous kidney tissues can be distinguished based on the mRNA level of the selected genes. Our results open new diagnostics possibilities where the mRNA signatures of tumour drivers can supplement the DNA-based approaches providing a more precise diagnostics opportunity leading to determine more precise therapeutic protocols.
Stomatal Regulation and Osmotic Adjustment in Sorghum in Response to Salinity
Authors: Dourado Pablo Rugero Magalhães , de Souza Edivan Rodrigues , Santos Monaliza Alves dos , Lins Cintia Maria Teixeira , Monteiro Danilo Rodrigues , Paulino Martha Katharinne Silva Souza , Schaffer Bruce
Published: May 2022

Sorghum bicolor (L.) Moench, one of the most important dryland cereal crops, is moderately tolerant of soil salinity, a rapidly increasing agricultural problem due to inappropriate irrigation management and salt water intrusion into crop lands as a result of climate change. The mechanisms for sorghum’s tolerance of high soil salinity have not been elucidated. This study tested whether sorghum plants adapt to salinity stress via stomatal regulation or osmotic adjustment. Sorghum plants were treated with one of seven concentrations of NaCl (0, 20, 40, 60, 80, or 100 mM). Leaf gas exchange (net CO2 assimilation (A), transpiration (Tr); stomatal conductance of water vapor (gs), intrinsic water use efficiency (WUE)), and water (Ψw), osmotic (Ψo), and turgor Ψt potentials were evaluated at 40 days after the imposition of salinity treatments. Plants exhibited decreased A, gs, and Tr with increasing salinity, whereas WUE was not affected by NaCl treatment. Additionally, plants exhibited osmotic adjustment to increasing salinity. Thus, sorghum appears to adapt to high soil salinity via both osmotic adjustment and stomatal regulation.
IAA Accumulation Promotes the Root Growth of Tea Plants under Aluminum
Authors: Gao Yaoyao , Wang Min , Shi Yifan , Yang Liu , Hu Jianhui , Fan Kai , Shi Yuanzhi
Published: May 2022

A moderate supply of aluminum (Al) can promote the root growth of plant species, such as tea, that have adapted to acid soils. The formation and development of roots are regulated by phytohormones. However, the role that phytohormones play in facilitating the beneficial effects of Al on root growth is unclear. In the present study, tea plants cultured hydroponically with either 0 or 0.4 mmol/L Al were characterized and compared in terms of root growth, hormone concentrations, and the expression of related genes. The hormone content in tea roots was measured by UPLC–MS, and the expression levels of genes involved in phytohormone biosynthesis, transport, and response were analyzed by qRT-PCR. Our results showed that the indole acetic acid (IAA) concentrations in the root base were significantly higher in plants supplied with Al compared to control plants without Al during the experimental period from 6 h to 7 d. By using inhibitors of IAA synthesis and transport, we found that Al induces short-term IAA synthesis in the root tip and enhances IAA transport in other parts of the root over the longer term, thus promoting root growth in tea plants by limiting IAA accumulation. The expression levels of genes YUC8 and AMI1, involved in IAA accumulation, were consistently upregulated in the root tip, whereas expression of PIN1/2/8, involved in IAA transport, was induced in the root base under Al supply at 6 h. In addition, the expression of PIN2 and PIN5 was highly induced in root stem by Al after 7 d and probably contributed to alleviating IAA accumulation in the root tip and thereby promoted root growth in tea plants. Meanwhile, the XET and expansin activities were increased under Al supply at 6 h. Thus, we conclude that the mechanism of Al-induced root growth is mediated by auxin accumulation and transport in cooperation with cell wall loosening in tea plants.
Effect of Providing Environmental Enrichment into Aviary House on the Welfare of Laying Hens
Authors: Son Jiseon , Lee Woo-Do , Kim Hee-Jin , Kang Bo-Seok , Kang Hwan-Ku
Published: May 2022

This study aimed to determine the effects of providing environmental enrichment materials—pumice stone and alfalfa hay—to laying hens in the aviary system. A total of 2196 40-week-old Hy-Line Brown laying hens were randomly allotted to three treatment groups: (1) no enrichment (control; CON), (2) enrichment with pumice stone (PS), and (3) enrichment with alfalfa hay (HAY). Each treatment comprised four replicates of 183 hens each, and four of the same materials were provided per replicate. The experiment lasted for 26 weeks. Feed and water were provided ad libitum. As a result, the PS and HAY groups demonstrated increased egg production (p < 0.001). The HAY group showed a reduced rate of mislaid eggs (p < 0.01) and produced low egg weight and pale-yellow yolk (p < 0.05). Both enrichment materials decreased blood creatinine (CRE) or lactate dehydrogenase (LDH) in the blood and resulted in a significantly lower corticosterone (CORT) level (p < 0.05). However, the feather condition scores for the laying hens were similar across all treatments (p > 0.05). In summary, although pumice stone and alfalfa hay are effective in alleviating stress and improving the production of laying hens, additional environmental improvement studies are needed to contribute to reducing pecking behaviors in poultry farming.
Ocular Surface Temperature in DED under Natural Non-Controlled Blinking Conditions
Authors: Rico-del-Viejo Laura , Llorens-Quintana Clara , Martínez-Alberquilla Irene , Madrid-Costa David , García-Montero María
Published: May 2022

Infrared (IR) thermography is a tool to non-invasively assess the tear film temperature. The aim was to analyze ocular surface temperature (OST) variations in dry eye disease (DED) and control eyes under natural non-controlled blinking conditions. Imaging was performed with a thermal camera (FLIR Systems Inc.) at 30 Hz framerate in 79 participants (39 DED (62.5% women, average age 48 ± 20 years) and 40 control (46.2 % women, average age 38 ± 13 years)) using non-contact IR thermography camera. Data acquisitions were performed in natural blinking conditions for 40 s. IR images were analyzed using a custom algorithm that calculates the OST indexes: mean OST, OST at the start and at the end, minimum and maximum OST, and tear evaporation rate (TER). No significant differences were found between groups in any thermal parameter analyzed (paired comparisons t-test, p > 0.05). In conclusion, the findings of this study did not reveal significant differences between DED and control eyes under natural non-controlled blinking conditions. However, the presence of clinical signs in the control group may affect the results, highlighting the role of DED diagnosis criteria.
The Effects of Local Pollution and Transport Dust on Aerosol Properties in Typical Arid Regions of Central Asia during DAO-K Measurement
Authors: Wei Yuanyuan , Li Zhengqiang , Zhang Ying , Li Kaitao , Chen Jie , Peng Zongren , Hu Qiaoyun , Goloub Philippe , Ou Yang
Published: May 2022

Dust aerosol has an impact on both the regional radiation balance and the global radiative forcing estimation. The Taklimakan Desert is the focus of the present research on the optical and micro-physical characteristics of the dust aerosol characteristics in Central Asia. However, our knowledge is still limited regarding this typical arid region. The DAO-K (Dust Aerosol Observation-Kashgar) campaign in April 2019 presented a great opportunity to understand further the effects of local pollution and transported dust on the optical and physical characteristics of the background aerosol in Kashgar. In the present study, the consistency of the simultaneous observations is tested, based on the optical closure method. Three periods dominated by the regional background dust (RBD), local polluted dust (LPD), and Taklimakan transported dust (TTD), are identified through the backward trajectories, combined with the dust scores from AIRS (Atmospheric Infrared Sounder). The variations of the optical and micro-physical properties of dust aerosols are then studied, while a direct comparison of the total column and near surface is conducted. Generally, the mineral dust is supposed to be primarily composed of silicate minerals, which are mostly very weakly absorbing in the visible spectrum. Although there is very clean air (with PM2.5 of 21 μg/m3), a strong absorption (with an SSA of 0.77, AAE of 1.62) is still observed during the period dominated by the regional background dust aerosol. The near-surface observations show that there is PM2.5 pollution of ~98 μg/m3, with strong absorption in the Kashgar site during the whole observation. Local pollution can obviously enhance the absorption (with an SSA of 0.72, AAE of 1.58) of dust aerosol at the visible spectrum. This is caused by the increase in submicron fine particles (such as soot) with effective radii of 0.14 μm, 0.17 μm, and 0.34 μm. The transported Taklimakan dust aerosol has a relatively stable composition and strong scattering characteristics (with an SSA of 0.86, AAE of ~2.0). In comparison to the total column aerosol, the near-surface aerosol has the smaller size and the stronger absorption. Moreover, there is a very strong scattering of the total column aerosol. Even the local emission with the strong absorption has a fairly minor effect on the total column SSA. The comparison also shows that the peak radii of the total column PVSD is nearly twice as high as that of the near-surface PVSD. This work contributes to building a relationship between the remote sensing (total column) observations and the near-surface aerosol properties, and has the potential to improve the accuracy of the radiative forcing estimation in Kashgar.
Exosomes Derived from BM-MSCs Mitigate the Development of Chronic Kidney Damage Post-Menopause via Interfering with Fibrosis and Apoptosis
Authors: Alasmari Wardah A. , Abdelfattah-Hassan Ahmed , El-Ghazali Hanaa M. , Abdo Samar A. , Ibrahim Doaa , ElSawy Naser A. , El-Shetry Eman S. , Saleh Ayman A. , Abourehab Mohammed A. S. , Mahfouz Hala
Published: May 2022

The rate of chronic kidney disease (CKD) is increasing globally, and it is caused by continuous damage to kidney tissue. With time the renal damage becomes irreversible, leading to CKD development. In females, post-menopause lack of estrogen supply has been described as a risk factor for CKD development, and studies targeting post-menopause CKD are scarce. In the present study, we used exosomes isolated from bone marrow mesenchymal stem/stromal cells (BM-MSCs) to test their therapeutic potential against the development of CKD. At first, the menopause model was achieved by surgical bilateral ovariectomy in female albino rats. After that, 100 µg of exosomes was given to ovariectomized rats, and the study continued for 2 months. Changes in urine volume, urine protein content, kidney function biochemical parameters (creatinine and BUN), kidney antioxidant parameters (SOD, GPx and CAT), histological changes, immunohistochemical levels of caspase 3, and the gene expression of NGAL (related to kidney damage), TGFβ1 and αSMA (related to fibrosis and EMT), and caspase 3 (related to apoptosis) were studied. After the ovariectomy, the occurrence of CKD was confirmed in the rats by the drastic reduction of serum estrogen and progesterone levels, reduced urine output, increased urinary protein excretion, elevated serum creatinine and BUN, reduced GPx SOD, and CAT in kidney tissue, degenerative and fibrotic lesions in the histopathological examination, higher immunohistochemical expression of caspase 3 and increased expression of all studied genes. After exosomes administration, the entire chronic inflammatory picture in the kidney was corrected, and a near-normal kidney structure and function were attained. This study shows for the first time that BM-MSCs exosomes are potent for reducing apoptosis and fibrosis levels and, thus, can reduce the chronic damage of the kidneys in females that are in their menopause period. Therefore, MSCs-derived exosomes should be considered a valuable therapy for preserving postmenopausal kidney structure and function and, subsequently, could improve the quality of females’ life during menopause.
Molecularly Imprinted Polymer-Amyloid Fibril-Based Electrochemical Biosensor for Ultrasensitive Detection of Tryptophan
Authors: Alam Ibrar , Lertanantawong Benchaporn , Sutthibutpong Thana , Punnakitikashem Primana , Asanithi Piyapong
Published: May 2022

A tryptophan (Trp) sensor was investigated based on electrochemical impedance spectroscopy (EIS) of a molecularly imprinted polymer on a lysozyme amyloid fibril (MIP-AF). The MIP-AF was composed of aniline as a monomer chemically polymerized in the presence of a Trp template molecule onto the AF surface. After extracting the template molecule, the MIP-AF had cavities with a high affinity for the Trp molecules. The obtained MIP-AF demonstrated rapid Trp adsorption and substantial binding capacity (50 µM mg−1). Trp determination was studied using non-Faradaic EIS by drop drying the MIP-AF on the working electrode of a screen-printed electrode. The MIP-AF provided a large linear range (10 pM–80 µM), a low detection limit (8 pM), and high selectivity for Trp determination. Furthermore, the proposed method also indicates that the MIP-AF can be used to determine Trp in real samples such as milk and cancer cell media.
Hormones and Vestibular Disorders: The Quest for Biomarkers
Authors: El Khiati Rhizlane , Tighilet Brahim , Besnard Stephane , Chabbert Christian
Published: May 2022

The vestibular system exerts control over various functions through neural pathways that are not yet fully mapped. Functional dysregulations or tissue lesions at different levels of the peripheral and the central vestibular networks can alter these different functions, causing a wide variety of symptoms, ranging from posturo-locomotor alterations to psychiatric syndromes such as PPPD, including the deregulation of the main biological functions. These different symptoms differ by their expression kinetics (they each appear and regress with their own kinetics) by the targets affected (muscles, organs, and brain areas) and by the sensitivity specific to each individual. Vestibular pathologies thus cover a mosaic of distinct effects, and they involve various effectors—which constitute the many markers of their different types and stages. It is therefore crucial, to predict the onset of a vertigo syndrome, to follow its temporal course, or to monitor the impact of therapeutic approaches, and to have specific and reliable biomarkers. Hormonal variations are among the possible sources of biomarkers for neurotology. We know that specific hormonal profiles can promote the appearance of vestibular disorders. We also know that the expression of vertigo syndrome is accompanied by measurable hormonal variations. The link between endocrine deregulation and vestibular alterations therefore no longer needs to be proven. However, there are still few data on their precise correlations with the vertigo syndrome. This study was undertaken with the aim to deliver an extensive review of the hormonal alterations linked to vestibular disorders. A review of the literature covering the last two decades was carried out using the MEDLINE and COCHRANE databases in order to identify studies associating the terms vestibular system or vestibular pathologies and hormones. Bibliographic data provides several outcomes in terms of therapeutic innovation in the diagnosis and therapeutic follow-up of vestibular pathologies.
Examination of Mixing Proportion in Self-Compacting Gangue-Based Pavement Concrete
Authors: Chen Lianjun , Guo Nan , Liu Guoming , Guo Xiaohan , Zhao Jipeng , Liu Zhaoxia
Published: May 2022

In recent years, with the rapid development of the coal-mining industry, the output of gangue has increased at a faster pace, while its utilization remains relatively low. The accumulation of a large amount of gangue has brought about a large environmental problem. In order to improve the utilization rate of waste gangue, and to solve the secondary environmental problems caused by gangue pollution, this paper conducted research on an economic and environmentally friendly gangue-based self-compacting concrete. This study designed aggregate industrial-analysis experiments to analyze the moisture content of the gangue and limestone, finding that the moisture content of gangue is 39% higher than that of limestone. By orthogonal experimental methods, the study investigated the fluidity, compressive strength, splitting strength and abrasion resistance of self-compacting gangue concrete. It was concluded that the optimal replacement rate of gangue for coarse aggregate is around 30%, the optimal replacement rate of fly ash for cement is around 30%, the optimal addition of polycarboxylate superplasticizer is 0.5% of the mass of cementitious materials, and the optimal rate of shear steel fibers is around 1% of the concrete capacity. In addition, this paper investigated the interfacial transition zone (ITZ) of the aggregate–cement slurry and found that the ITZ of gangue aggregate and cement mortar is more likely to generate AFT crystals, which will contribute more to the improvement of the strength of concrete in the early stage. In addition, a field-effect analysis was carried out in this study, and it found that gangue-based self-compacting concrete, as an environmentally friendly material, can basically meet the design requirements of C30 paving concrete.
Epidemiology, Risk Factors and Diagnosis of Small Bowel Adenocarcinoma
Authors: Aparicio Thomas , Pachev Atanas , Laurent-Puig Pierre , Svrcek Magali
Published: May 2022

Adenocarcinomas of the small intestine are rare tumors but their incidence is increasing. There is a slight male predominance. The median age at diagnosis is the 6th decade. The most frequent primary location is the duodenum. There is no clearly identified environmental risk factor, but adenocarcinomas of the small intestine are associated in almost 20% of cases with predisposing diseases (Crohn’s disease, Lynch syndrome, familial adenomatous polyposis, Peutz–Jeghers syndrome and celiac disease).
Upper Gastrointestinal Cancer and Liver Cirrhosis
Authors: Jeng Kuo-Shyang , Chang Chiung-Fang , Sheen I-Shyan , Jeng Chi-Juei , Wang Chih-Hsuan
Published: May 2022

The extended scope of upper gastrointestinal cancer can include esophageal cancer, gastric cancer and pancreatic cancer. A higher incidence rate of gastric cancer and esophageal cancer in patients with liver cirrhosis has been reported. It is attributable to four possible causes which exist in cirrhotic patients, including a higher prevalence of gastric ulcers and congestive gastropathy, zinc deficiency, alcohol drinking and tobacco use and coexisting gut microbiota. Helicobacter pylori infection enhances the development of gastric cancer. In addition, Helicobacter pylori, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans also contribute to the development of pancreatic cancer in cirrhotic patients. Cirrhotic patients (especially those with alcoholic liver cirrhosis) who undergo liver transplantation have a higher overall risk of developing de novo malignancies. Most de novo malignancies are upper gastrointestinal malignancies. The prognosis is usually poor. Considering the surgical risk of upper gastrointestinal cancer among those with liver cirrhosis, a radical gastrectomy with D1 or D2 lymph node dissection can be undertaken in Child class A patients. D1 lymph node dissection can be performed in Child class B patients. Endoscopic submucosal dissection for gastric cancer or esophageal cancer can be undertaken safely in selected cirrhotic patients. In Child class C patients, a radical gastrectomy is potentially fatal. Pancreatic radical surgery should be avoided in those with liver cirrhosis with Child class B or a MELD score over 15. The current review focuses on the recent reports on some factors in liver cirrhosis that contribute to the development of upper gastrointestinal cancer. Quitting alcohol drinking and tobacco use is important. How to decrease the risk of the development of gastrointestinal cancer in those with liver cirrhosis remains a challenging problem.
Reducing Chemotherapy-Induced DNA Damage via nAChR-Mediated Redox Reprograming—A New Mechanism for SCLC Chemoresistance Boosted by Nicotine
Authors: Wang Yuzhi , Bian Tengfei , Song Lina , Jiang Yunhan , Huo Zhiguang , Salloum Ramzi G. , Warren Graham W. , Kaye Frederic J. , Fujioka Naomi , Jin Lingtao , Xing Chengguo
Published: May 2022

Up to 60% of patients with small cell lung cancer (SCLC) continue to smoke, which is associated with worse clinical outcomes. Platinum-based chemotherapies, in combination with topoisomerase inhibitors, are first-line therapies for SCLC, with rapid chemoresistance as a major barrier. We provided evidence in this study that nicotine and its major metabolite, cotinine, at physiologically relevant concentrations, reduced the efficacy of platinum-based chemotherapies and facilitated chemoresistance in SCLC cells. Mechanistically, nicotine or cotinine reduced chemotherapy-induced DNA damage by modulating cellular redox processes, with nAChRs as the upstream targets. Surprisingly, cisplatin treatment alone also increased the levels of nAChRs in SCLC cells, which served as a self-defense mechanism against platinum-based therapies. These discoveries were confirmed in long-term in vitro and in vivo studies. Collectively, our results depicted a novel and clinically important mechanism of chemoresistance in SCLC treatment: nicotine exposure significantly compromises the efficacy of platinum-based chemotherapies in SCLC treatment by reducing therapy-induced DNA damage and accelerating chemoresistance acquisition. The results also emphasized the urgent need for tobacco cessation and the control of NRT use for SCLC management.
RR Myelo POINT: A Retrospective Single-Center Study Assessing the Role of Radiotherapy in the Management of Multiple Myeloma and Possible Interactions with Concurrent Systemic Treatment
Authors: Guerini Andrea Emanuele , Tucci Alessandra , Alongi Filippo , Mataj Eneida , Belotti Angelo , Borghetti Paolo , Triggiani Luca , Pegurri Ludovica , Pedretti Sara , Bonù Marco , Tomasini Davide , Imbrescia Jessica , Donofrio Alessandra , Facheris Giorgio , Singh Navdeep , Volpi Giulia , Tomasi Cesare , Magrini Stefano Maria , Spiazzi Luigi , Buglione Michela
Published: May 2022

Background and purpose: Although chemotherapy, biological agents, and radiotherapy (RT) are cornerstones of the treatment of multiple myeloma (MM), the literature regarding the possible interactions of concurrent systemic treatment (CST) and RT is limited, and the optimal RT dose is still unclear. Materials and methods: We retrospectively analyzed the records of patients who underwent RT for MM at our institution from 1 January 2005 to 30 June 2020. The data of 312 patients and 577 lesions (treated in 411 accesses) were retrieved. Results: Most of the treated lesions involved the vertebrae (60%) or extremities (18.9%). Radiotherapy was completed in 96.6% of the accesses and, although biologically effective doses assuming an α/β ratio of 10 (BED 10) > 38 Gy and CST were significantly associated with higher rates of toxicity, the safety profile was excellent, with side effects grade ≥2 reported only for 4.1% of the accesses; CST and BED 10 had no impact on the toxicity at one and three months. Radiotherapy resulted in significant improvements in performance status and in a pain control rate of 87.4% at the end of treatment, which further increased to 96.9% at three months and remained at 94% at six months. The radiological response rate at six months (data available for 181 lesions) was 79%, with only 4.4% of lesions in progression. Progression was significantly more frequent in the lesions treated without CST or BED 10 < 15 Gy, while concurrent biological therapy resulted in significantly lower rates of progression. Conclusion: Radiotherapy resulted in optimal pain control rates and fair toxicity, regardless of BED 10 and CST; the treatments with higher BED 10 and CST (remarkably biological agents) improved the already excellent radiological disease control.
Essential Amino Acid Intake Is Required for Sustaining Serum Insulin-like Growth Factor-I Levels but Is Not Necessarily Needed for Body Growth
Authors: Nishi Hiroki , Uchida Kaito , Saito Maki , Yamanaka Daisuke , Nagata Haruka , Tomoshige Hinako , Miyata Ichiro , Ito Koichi , Toyoshima Yuka , Takahashi Shin-Ichiro , Hakuno Fumihiko , Takenaka Asako
Published: May 2022

Essential amino acids (EAAs) are those that cannot be synthesized enough to meet organismal demand; therefore, it is believed that they must be taken from the diet for optimal growth. The growth hormone (GH)/insulin-like growth factor-I (IGF-I) system is also considered significant for growth regulation in mammals. This study aimed to evaluate the relative contributions of protein nutrition and the GH/IGF-I system to body growth regulation. Experiments using rodents and hepatocyte-derived cell lines subjected to EAA deficiency showed that a reduction in the serum EAA concentration hinders Igf1 transcription in the liver in a cell-autonomous manner, thereby decreasing serum IGF-I levels. Remarkably, when the serum IGF-I level of mice on a low-protein diet was restored by the recombinant IGF-I infusion, the body growth was mostly rescued, although the mice were still deficient in EAA intake. Meanwhile, the GH signal activation and subsequent Igf1 transcription were also dramatically diminished by EAA deprivation in the cell culture model. Altogether, we demonstrate that EAAs are not strictly necessary for animal growth as building blocks but are required as IGF-I-tropic cues. The results will bring a paradigm shift regarding the definition of “essential” amino acids.