bluuebunny/crossref_metadata_2025 huggingface dataset:
The activation of AIM2/Caspase-1/GSDMD pathway contributes to pyroptosis of keratinocytes in Stevens-Johnson syndrome/toxic epidermal necrolysis
Authors: Yang Lu , Shou Yanhong , Li Feng , Du Juan , Zhu Xiao-hua , Wang Shang-shang , Xie Lin , Zhou Li-juan , Zhou Jia-qing , Yang Yong-sheng , Xu Jinhua
Published: January 2023

Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) are severe cutaneous adverse drug reactions, rare but life-threatening, characterized by massive keratinocyte death and development of a systemic inflammatory response. Pyroptosis is a lytic form of pro-inflammatory programmed necrotic cell death which participates in the pathogenesis of various inflammatory diseases, it depends on activation of inflammasomes such as NLRP3/NALP1/AIM2/NLRC4 and inflammatory caspases (caspase 1/4/5), subsequently it is executed by pore-forming protein of the gasdermin family, resulting in releases of inflammatory cytokines such as IL-1β, IL-18. However, does pyroptotic machinery exist in the SJS/TEN remains open. In our study, we present evidence for the involvement and mechanism of pyroptosis in SJS/TEN keratinocytes. Inflammatory cytokines TNF-α and IFN-γ participating in keratinocyte pyroptosis through strengthening of cytoplasmic dsDNA/AIM2/Caspase-1/GSDMD pathway were identified in vivo and in vitro. Further, we observed that all of the four small molecular pyroptosis inhibitors including Z-YVAD-FMK，dimethyl fumarate (DMF), necrosulfonamide (NSA) and disulfiram caused a significant inhibition of pyroptosis in human primary keratinocytes, and significantly downregulated the release of pyroptosis-related inflammatory cytokines IL-1β and IL-18. These discoveries expand our understanding of the pathogenesis of SJS/TEN and it is hoped that this could facilitate the development of new therapeutics for the treatment of SJS/TEN patients with activated pyroptosis in keratinocytes.
Divergent epigenetic responses to birth asphyxia in severe mental disorders
Authors: Wortinger Laura , Stavrum Anne-Kristin , Shadrin Alexey , Szabo Attila , Rukke Sondre Høeg , Nerland Stener , Smelror Runar , Jørgensen Kjetil , Barth Claudia , Andreou Dimitrios , Weibell Melissa , Djurovic Srdjan , Andreassen Ole , Thoresen Marianne , Ursini Gianluca , Agartz Ingrid , Hellard Stephanie Le
Published: January 2023

Epigenetic modifications influenced by environmental exposures are molecular sources of phenotypic heterogeneity found in schizophrenia and bipolar disorder and may contribute to shared etiopathogenetic mechanisms of these two disorders. Newborns who experienced birth asphyxia have suffered reduced oxygen delivery to the brain around the time of birth, which increases the risk of later psychiatric diagnosis. This study aimed to investigate DNA methylation in blood cells for associations with a history of birth asphyxia, a neurologically harmful condition occurring within the biological environment of birth. We utilized prospective data from the Medical Birth Registry of Norway to identify incidents of birth asphyxia in 643 individuals with schizophrenia or bipolar disorder and 676 healthy controls. We performed an epigenome wide association study to distinguish differentially methylated positions associated with birth asphyxia. We found an interaction between methylation and exposure to birth asphyxia on case–control status, wherein having a history of birth asphyxia was associated with an increase of methylation in healthy controls and a decrease of methylation in patients on 4 regions of DNA important for brain development and function. The differentially methylated regions were observed in genes involved in oligodendrocyte survival and axonal myelination and functional recovery(LINGO3); assembly, maturation and maintenance of the brain (BLCAP;NNATandNANOS2) and axonal transport processes and neural plasticity (SLC2A14). These findings are consistent with the notion that an opposite epigenetic response to birth asphyxia, in patients compared with controls, may contribute to molecular mechanisms of risk for schizophrenia and bipolar disorder.
Erector spinae muscle area and the ratio of the computed tomography values of the liver and spleen are associated with frailty in older patients with diabetes: A cross-sectional study
Authors: Sato Motoya , Tamura Yoshiaki , Murao Yuji , Yorikawa Fumino , Katsumata Yuu , Watanabe So , Zen Shugo , Kodera Remi , Oba Kazuhito , Toyoshima Kenji , Chiba Yuko , Araki Atsushi
Published: January 2023

Background
Older patients with diabetes mellitus are more susceptible to frailty. Although some imaging markers of appendicular skeletal muscle mass obtained using dual-energy X-ray absorptiometry or computed tomography (CT) imaging can reflect frailty status, the association between imaging indices obtained by abdominal CT scans and frailty in older inpatients has not been reported.
Methods
A total of 151 older inpatients with diabetes mellitus (median age, 79 years; men, 42%) who underwent abdominal CT scans close to the admission were studied to examine the associations between abdominal CT indices and frailty. We used two frailty definitions: the modified Cardiovascular Health Study (mCHS) criteria and Kihon Checklist (KCL) criteria. Using the imaging analysis software SYNAPSE VINCENT®, we compared the areas of four truncal muscles (erector spinae, iliopsoas, rectus abdominis, and abdominal oblique muscles) and the ratio of the CT values of the liver and spleen (L/S) between frail and non-frail patients. The muscle areas that showed the strongest associations with frailty were also investigated in relation to grip strength and walking speed. Finally, we performed multivariate binominal logistic regression analysis to assess the independent associations of muscle areas and L/S with the prevalence of frailty.
Results
The prevalence of frailty defined by the mCHS and KCL criteria was 55% and 52%, respectively. The area of the erector spinae muscle was most significantly associated with frailty, and was significantly smaller in mCHS-defined frail patients of both sexes and in men with KCL-defined frailty. The erector spinae muscle area was also positively correlated with grip strength and walking speed. In contrast, the L/S ratio was higher in men with KCL-defined frailty. Multivariate logistic regression analyses revealed that the area of the erector spinae muscle was independently associated with mCHS-defined frailty in women, and the L/S ratio was associated with KCL-defined frailty in men.
Conclusions
The erector spinae muscle area and low liver fat could be indices of frailty in older patients with diabetes.
Effect of Square Dance Exercise on Middle-aged and Older Women With Persistent Postural-Perceptual Dizziness (PPPD)
Authors: tang Bo , jiang wei , zhang chuang , tan hong , luo minghua , he yuqin , yu xiaojun
Published: January 2023

Background: Persistent postural-perceptual dizziness (PPPD) is a disorder of vestibular dysfunction in which chronic dizziness leads to limitations in daily life. Although pharmacology, vestibular rehabilitation, and cognitive behavioral therapy have been suggested to have some efficacy, they have certain limitations. Some patients with PPPD report that square dance can effectively relieve the symptoms of dizziness and instability, and their mood also improves. However, to date, there has been no research about the effect of square dance Exercise on people of PPPD，not to mention the middle-aged and older women who are more interested in square dance.
Objective: To evaluate the effects of square dance on the subjective sensations of dizziness, balance enhancement, anxiety, and depressive symptom regulation in middle-aged and older women with PPPD.
Materials and Methods: In this trial, a total of 124 patients diagnosed with PPPD were enrolled, among whom 64 were randomly assigned to square dance training as the experimental group (EG), and the other 60 cases were the control group (CG) without square dance training. To evaluate the effect of square dancing on middle-aged and elderly women with PPPD, data from the Dizziness Handicap Inventory (DHI), Hospital Anxiety and Depression Scale (HADS), Active-specific Balance Confidence Scale (ABC), and Vestibular Disorder Activities of Daily Living Scale (VADL) were collected and compared at the beginning and at three and six months of the trial.
Results: Before the trial, there were no significant differences between the EG and CG. Compared with baseline measures, DHI, HADS, ABC, and VADL scores improved as the experiment progressed, and the improvements were more pronounced in the EG.
Conclusion: Square dance has a positive impact on the subjective sensation of dizziness, balance enhancement, anxiety, and depressive symptom regulation in middle-aged and older women with PPPD.
FDG-PET/CT for lymph node staging in bladder cancer treated with radical cystectomy
Authors: Pihl Vilhelm , Markus Maria , Abrahamsson Johan , Bläckberg Mats , Hagberg Oskar , Kollberg Petter , Simoulis Athanasios , Trägårdh Elin , Liedberg Fredrik
Published: January 2023

Background18F-Flourodeoxyglucose positron emission combined with computed tomography (FDG-PET/CT) has been proposed to improve preoperative staging in patients with bladder cancer subjected to radical cystectomy (RC). Objective Our aim was to assess the accuracy of FDG-PET/CT for lymph node staging compared to lymph node status in the surgical lymphadenectomy specimen obtained at RC, and to explore potential factors associated with false positive FDG-PET/CT results. Design, setting and participants Consecutive patients with bladder cancer undergoing RC with extended lymph node dissection between 2011 and 2019 without preoperative chemotherapy in a tertial referral cystectomy unit were included in the study. Outcome measurements and statistical analyses Sensitivity, specificity, positive and negative predictive values, and likelihood ratios were calculated. Potential factors investigated for association with false positive FDG-PET/CT were; bacteriuria within four weeks prior to FDG-PET/CT, Bacillus Calmette-Guerin (BCG) treatment within 12 months prior to FDG-PET/CT and transurethral resection of bladder tumor (TURB) within four weeks prior to FDG-PET/CT. Results Among 159 patients included for analysis, 46 (29%) were clinically node positive according to FDG-PET/CT. The sensitivity and specificity for detection of lymph node metastasis were 50% and 82%, respectively, and the corresponding positive predictive and negative predictive values were 59% and 76%. Positive and negative likelihood ratios were 2.8 and 0.6, respectively. No association were found between bacteriuria, previous BCG treatment or TURB within 28 days and false positive FDG-PET/CT results. Conclusions Preoperative FDG-PET/CT prior to RC had a clinically meaningful high specificity (82%) but lower sensitivity (50%) for detection of lymph node metastases compared to lymph node status in an extended pelvic lymphadenectomy template. We could not identify any factors associated with false positive FDG-PET/CT outcomes.
Indoor Positioning Design Based on HCC in the Background of Mobile Edge ComputingIndoor Positioning Design Based on HCC in the Background of Mobile Edge Computing
Authors: Ge Tai
Published: January 2023

In the subject of human-centered computing (HCC), achieving accurate location in an indoor setting is a crucial research problem and a hotspot for research. In comparison to WiFi, GPS, and Bluetooth location technologies, the radio frequency identification (RFID) technology obviously has benefits. However, the fixed path loss coefficient in the indoor positioning algorithm based on RFID cannot accurately reflect the complex and mutable properties of inside settings, leading to significant indoor positioning mistakes and sluggish speeds. This research suggests an indoor positioning system based on Edge Computing (EC) to improve the precision and responsiveness of indoor positioning. First, the indoor signal strength is calculated using RFID. Next, the output is sent to a neural network, which outputs the path loss coefficient. Finally, the EC is used to create a more effective indoor positioning system design. According to experiments, the suggested method can more correctly reflect inside environment signal changes and increase indoor location accuracy. This solution effectively satisfies the requirements for indoor real-time positioning even after being deployed to EC.
Relationship between Determinant Factors of Tooth Decay and Filling in Indonesia (Mapping Determinant Factors using Geographic Information System)
Authors: Lestari Ayu Asri , Darwita Risqa Rina , Adiatman Melissa , Rahardjo Anton , Ramadhani Atik , Bramantoro Taufan
Published: January 2023

Background One of the goals of Indonesia's participation in sustainable development goals to improve its health status. Efforts made to achieve health improvement are increasing the availability of healthcare facilities so people can easily access and get treatment for dental and oral health. This study aimed to determine the relationship between sociodemographic and healthcare services factors that affect tooth decay and filling in Indonesia. Methods A cross-sectional study using secondary data from Basic Health Research 2018 as classified based on WHO age as much 14.031 subjects are sociodemographic factors, utilization of dental visits, total decay, and filling. Health Facility Research 2019 data using 17.741 healthcare facilities. The number of dentists as much as 22.926 data through the Indonesia Medical Council on December 2021. These variables were tested statistically and mapped using QGIS. Results Mann-Whitney test showed a significant difference (p < 0,05) between the female sex, living in the urban area towards tooth decay and filling. Kruskal-Wallis test showed a significant difference (p < 0,05) between groups of informal, formal workers with unemployment, educational level in 35–44 years group between no school and elementary-high school with diploma 3-college and group of 65 + years between all groups, and also frequency utilization towards tooth decay and tooth filling had significant statistics. Spearman test showed a correlation statistically (p < 0,05) between the number and ratio of healthcare facilities and dentists towards tooth decay and filling. Conclusion In this study, there is a relationship between sociodemographic factors and healthcare services that affect tooth decay and filling. Efforts to equitable distribution of healthcare facilities and dentists, as well as an intervention to increase utilization by looking at all aspects of sociodemographic characteristics.
The Design of College Student Achievement Management System based on GA-BP Network
Authors: Liu Xia , Guo Jing , Jiang Jingjing
Published: January 2023

With the development of computer technology, higher education and teaching work gradually depend on the score management system. The performance management system has a substantial amount of student curriculum performance data, and how to effectively analyze these data to improve the teaching quality of the school is a problem worthy of in-depth study. This study integrates a genetic algorithm and a neural network on the basis of the BP neural network to create a GA-BP network hybrid algorithm that addresses the BP neural network's issues, such as its proclivity to fall into local minima and slow convergence speed. The GA-BP network hybrid algorithm improves the connection weights and thresholds of the BP network using the genetic algorithms global search capability so that the network can search from a better initial value and complete the global optimization more quickly. According to the needs of student achievement management, this research designed and developed a student achievement management system and realized the application of the GA-BP network in the student achievement management system. Through the experiments of MATLAB software simulation, this study has verified the feasibility of the model applied to the graduation score prediction. The results show that the model can make a more accurate prediction of the unknown graduation scores by using the existing student course scores, so as to use the prediction results to carry out academic warning prompts for students, help students strengthen their professional knowledge learning, and help colleges and universities adjust their education and teaching work.
A new Lean tool for efficiency evaluation in SMED projects
Authors: BRAGLIA Marcello , Paco Francesco Di , MARRAZZINI Leonardo
Published: January 2023

The ability of companies to rapidly conduct a changeover from one product to another as part of a production process is a fundamental step towards a more flexible production system that can deal with an increasingly dynamic and competitive market. Single-Minute Exchange of Die (SMED) is the best-known lean tool that aims to reduce time consumption in the changeover process. This paper presents a new Lean tool called Set-up Saving Deployment (SSD), which improves set-up efficiency by classifying, analyzing, and removing set-up losses within a changeover process, and which supports decision-making for SMED implementation. SSD uses three matrices, constructed sequentially from the first (L-Matrix) to the last (ECE-Matrix), in order to assess the possible time savings that can be achieved by eliminating losses and, in addition, to forecast possible improvements resulting from implementing a SMED project. SSD also provides a new basket of tailored set-up efficiency indicators that allow the analysis team to correctly assess set-up efficiency, and compare the 'as is' condition with the subsequent 'to be' condition from an operational perspective. The effectiveness of SSD in addressing set-up losses and predicting time savings is illustrated using an industrial case study of a resin doming machine. Thanks to its structured step-by-step procedure, SSD significantly improves the efficiency of the changeover process.
Structural and Reactivity Effects of Secondary Metal Doping into Iron-Nitrogen-Carbon Catalysts for Oxygen Electroreduction
Authors: Jaouen Frédéric , Luo Fang , Roy Aaron , Sougrati Moulay Tahar , Khan Anastassiya , Cullen David , Wang Xingli , Primbs Mathias , Zitolo Andrea , Strasser Peter
Published: January 2023

While improved activity was recently reported for bimetallic iron-metal-nitrogen-carbon (FeMNC) catalysts for the oxygen reduction reaction (ORR) in acid medium, the nature of active sites and interactions between the two metals are poorly understood. Here, FeSnNC and FeCoNC catalysts were structurally and catalytically compared to their parent FeNC and SnNC catalysts. While CO cryo-chemisorption revealed a twice lower site density of M-Nx sites for FeSnNC and FeCoNC relative to FeNC and SnNC, the mass activity of both bimetallic catalysts is 50–100% higher than that of FeNC, due to a larger turnover frequency in the bimetallic catalysts. Electron microscopy and X-ray absorption spectroscopy identified the coexistence of Fe-Nx and Sn-Nx or Co-Nx sites, while no evidence was found for binuclear Fe-M-Nx sites. 57Fe Mössbauer spectroscopy revealed that the bimetallic catalysts feature a higher D1/D2 ratio of the spectral signatures assigned to two distinct Fe-Nx sites, relative to the FeNC parent catalyst. Thus, the addition of the secondary metal favored the formation of D1 sites, associated with the higher turnover frequency.
Diastolic function evaluation in children with ventricular arrhythmia.
Authors: Pietrzak Radosław , Książczyk Tomasz M , Franke Magda , Werner Bożena
Published: January 2023

Premature ventricular contractions(PVCs) are frequently seen in children. The diastolic function has not been investigated in PVC children. We evaluated the left ventricular diastolic function in PVC children with normal left ventricular systolic function to detect whether potential diastolic function disturbances influence physical performance. In the study group (36 PVCs children) and the controls (33 healthy volunteers). Echocardiographical diastolic function parameters such as left atrial volume index(LAVI), left atrial strains (AC-R, AC-CT, AC-CD), E wave, E’ medial atrial tissue doppler velocity, E/E’ and isovolumic relaxation time (IVRT) were measured. In the CPET, oxygen uptake (VO2max) was registered. Evaluation of diastolic function parameters revealed statically significant differences between the study and control group regarding Edt 176.58 ± 54.8ms vs 136.94 ± 27.8ms,p < 0.01; E/E’12.6 ± 3.0 vs. 6.7 ± 1.0,p < 0.01; IVRT 96.6 ± 19.09ms. vs. 72.86 ± 13.67 ms,p < 0.01, respectively. Left atrial function was impaired in the study group compared to controls: LAVI 25.3 ± 8.2ml/m2 vs. 19.2 ± 7.5ml/m2,p < 0.01, AC-CT 34.8 ± 8.6% vs. 44.8 ± 11.8%,p < 0.01; AC-R-6.0 ± 4.9% vs. -11.5 ± 3.5%, p < 0.01, respectively. Statistically significant moderate, negative correlation between VO2max and E/E’(r =-0.33, p = 0.02) was found. Left ventricular diastolic function is impaired and deteriorates with the arrhythmia burden increase in PVC children. Ventricular arrhythmia in young individuals may be related to the filling pressure elevation and drive to exercise capacity deterioration.
Approximate Isosceles ω-Orthogonality and Approximate ω-Parallelism
Authors: AMYARI Maryam , TORABIAN Maryam , KHIBARY Marzieh MORADIAN
Published: January 2023

We introduce two notions of approximate isosceles ω-orthogonality and two notions of approximate ω-parallelism for bounded linear operators on a Hilbert space. Moreover , we state some basic properties of these notions and investigate some relations between them.
2010 Mathematics Subject Classification. Primary 47A12; 46B20; 46C05.
The PREDG study: a randomised controlled trial testing whether an educational intervention can prevent gestational weight gain in women with obesity
Authors: Barquiel Beatriz , Calvo Mercedes , Moreno-Domínguez Óscar , Martínez-Sánchez Nuria , Muner Marta , Bedate María Francisca , Delgado María , López Silvia , Hillman Natalia , González Noemí , Calle María De la , Bartha José Luis
Published: January 2023

OBJECTIVE The PREDG trial was designed to study the influence of an educative program on gestational weight gain in women with pregestational obesity.
METHODS Randomized controlled clinical trial (ISRCTN61793947) in 169 women with pregestational obesity (BMI ≥30 kg/m2). Women were randomized to participate in a group education program in nutrition and physical activity or conventional follow-up in a specialized Unit of Obesity and Pregnancy. The nutritional intervention was adjusted to prepregnancy BMI and to the physical activity intensity. Quality was based on the Mediterranean diet. Macronutrients were distributed as follows: 50% carbohydrates, 20% protein and 30% fat. Adequate gestational weight gain was defined between 5-9 kg (IOM 2009). Mean gestational weight gain was compared between groups by using the T Student test and frequencies of adequate gestational weight gain were compared by using ꓫ2.
RESULTS Gestational weight gain was lower in the intervention group: 8 (4-11) vs 9.2 (6-13) kg, p 0.026. Gestational weight gain was below 9 kg in 24 (61.5%) women of the intervention vs 10 (24.4%) of the control group (p 0.001). Regarding obstetric complications, there were 15 (8.3%) cases of gestational diabetes with no differences between the groups. There were 14 (18.2%) cases of gestational hypertension or preeclampsia in the intervention group compared with 26 (32.5%) in the control group (p 0.040). With reference to neonatal weight, there were 7 (31.8%) large for gestational age neonates in the intervention group compared with 15 (68.2%) in the control group (p 0.050). CONCLUSIONS A group-based educative and structured intervention results in an adequate weight gain and lower rates of gestational hypertension, preeclampsia and large for gestational age neonates in pregnant women with obesity.
Shoot and root residues decomposition from different cropping systems under semiarid Mediterranean conditions
Authors: Brull Irene Martin , Cantero-Martínez Carlos , Franco-Luesma Samuel , Lafuente Victoria , Álvaro-Fuentes Jorge
Published: January 2023

Aims: Improving the management of crop residues is essential for water and soil conservation and for increasing soil C and N levels in dryland agroecosystems. The main objective of the study was to evaluate the decomposition dynamics and nutrient release from crop residues from different cropping systems in a semiarid Mediterranean agroecosystem.
Methods: A litterbag experiment was conducted from July of 2020 to June of 2021 to examine the shoot and root decomposition dynamics of vetch, pea and barley residues in different cropping systems: a barley continuous monoculture, B(B), and the two phases of two crop rotations: barley in rotation with vetch, B(V) and V(V); barley in rotation with barley, B(P) and P(B).
Results: From week 9 to 14, a general decay of residues was observed caused by the higher soil moisture and temperatures. After 48 weeks of decomposition, a 41% and 60% of mass remaining was found corresponding to vetch and pea surface residues respectively, whilst barley mass remaining ranged 87-76% depending on the cropping system. In root residues, the mass decay from legume residues (40-44%) was higher compared to barley residues (17-29%). Exponential decay and linear models explained the residues decomposition observed in our study conditions. The C to N ratio of residues and edaphoclimatic conditions played a major role controlling the decomposition and nutrient loss rates.
Conclusion: According to our results, in Mediterranean semiarid agroecosystems, residue decomposition and nutrient release dynamics from different crop residues need to be considered for improving the sustainability of the cropping systems.
Stability Analysis of Milling Chatter in Six-Degree-of-Freedom Industrial Robots
Authors: Zhang Yun , Zhou Tingting , Hu Tianliang , Qiao Jinwei
Published: January 2023

Due to the low structural rigidity of six-DOF industrial robots, the chattering phenomenon is prone to occur under the excitation of periodic milling force, which has an effect on the processing quality of workpieces. In this paper, the influence of milling parameters on the chatter stability of six-DOF robot milling is studied, under the consideration of the modal coupling effect and the regenerative effect on milling stability. First, a modal equation considering both the modal coupling effect and the regenerative effect is developed. Second, based on the fully discrete method, the chatter stability domain model of six-DOF robot (namely, the stability lobe diagram) is established, and the influences of the axial depth of cut, radial depth of cut, and feed rate on the stability of the machining system are analyzed. Finally, the accuracy of the prediction results of the chatter stability domain model is verified by milling experiments.
Effect of load pattern used for seismic design on the collapse capacity of steel moment frames
Authors: Farzaneh Sara , Khosravi Horr
Published: January 2023

Well-known design codes use different lateral load patterns for Equivalent Lateral Force (ELF) procedure. However, the influence of using different load patterns on the collapse capacity of structures has rarely been investigated. It is obvious that using different lateral load patterns for design leads to different stiffness and strength distribution in the height of the structure and affects the collapse capacity of the structure. This paper aims to study the influence of designing structures with different lateral load patterns on their collapse capacity. For this purpose, three steel moment-resisting frames (SMRFs) have been designed with 6 different lateral load patterns. The structures are designed in such a way that all the structures designed with different load patterns have the same weight. Incremental dynamic analysis (IDA) and fragility analysis are performed for all structures and the median collapse intensities are compared. The results of this study clarify the significant effect of designing with different lateral load patterns on the collapse probability of some structures.
Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid
Authors: Ge Haoyu , Qiao Jie , Xu Hao , Liu Ruishan , Zhao Junhui , Chen Ruyan , Li Chenyu , Guo Xiaobing , Zheng Beiwen
Published: January 2023

Background: Although Klebsiella pneumoniae Carbapenemase (KPC) -type class A β-lactamases spread widely throughout the world, KPC-3-producing isolates are rarely reported in China. This study aims to explore the emergence, antibiotic resistance profiles, and plasmid characteristics of blaKPC-3-carrying Pseudomonas aeruginosa.
Methods: Species identification was performed by MALDI-TOF-MS, and antimicrobial resistance genes (ARGs) were identified by polymerase chain reaction (PCR). The characteristics of the target strain were detected by whole-genome sequencing (WGS) and antimicrobial susceptibility testing (AST). Plasmids were analyzed by S1-nuclease pulsed-field gel electrophoresis(S1-PFGE), Southern blotting and transconjugation experiment.
Results: Five P. aeruginosa strains carrying blaKPC-3 were isolated from two Chinese patients without a history of travelling to endemic areas. All strains belonged to the novel sequence type ST1076. The blaKPC-3 was carried on a 395-kb IncP-2 megaplasmid with a conserved structure (IS6100-ISKpn27-blaKPC-3-ISKpn6-korC-klcA), and this genetic sequence was identical to many plasmid-encoded KPC of Pseudomonas species. By further analyzing the genetic context, it was supposed that the original of blaKPC-3 in our work was a series of mutation of blaKPC-2.
Conclusions: The emergence of a multidrug resistance IncP-2 megaplasmid and clonal transmission of blaKPC-3-producing P. aeruginosa in China underlined the crucial need for continuous monitoring of blaKPC-3 for prevention and control of its further dissemination in China.
Accumulation of oxysterols in the erythrocytes of COVID-19 patients as a biomarker for case severity
Authors: Khedr Alaa , Khayat Maan , Khayyat Ahdab , Asfour Hany , Alsilmi Rahmah , Kammoun Ahmed
Published: January 2023

Background: Due to the high risk of COVID-19 patients to the formation of thrombosis in the circulating blood, atherosclerosis, and myocardial infarction, it was necessary to study the lipidomic of the erythrocytes. The aim of this work was to analyze the pathogenic oxysterols and acylcarnitines in the erythrocyte’s homogenate of COVID-19 patients and to estimate the case severity from the level of oxysterols.
Methods: A linear ion trap mass spectrometry coupled with high-performance liquid chromatography was used to investigate the extract of erythrocytes homogenate. The toxic biomarkers that primarily induce the generation of dead red blood cells, were characterized, and quantified in the erythrocytes of COVID-19 patients and matched with healthy volunteers.
Results: A total of 30 patients and 30 healthy volunteers were enrolled. The concentration of five oxysterols and six acylcarnitines in the erythrocyte’s homogenate of COVID-19 patients was significantly upregulated matching with healthy subjects at p <0.05. The average total concentration of oxysterols was 23.36 ± 13.47 μg/mL in the erythrocytes of COVID-19 patients, while samples of healthy volunteers showed a total concentration of 4.92 ± 1.61 μg/mL. The average concentration level of 7-ketocholesterol and 4-cholestenone in the COVID-19 patients was higher by five and ten-fold compared to the healthy subjects. Also, the average concentration of acylcarnitines in the erythrocyte's homogenate of COVID-19 patients was high by 2-to-4-fold in comparison with the healthy volunteers.
Conclusions: The abnormally high levels of oxysterols and acylcarnitines found in the erythrocytes of COVID-19 patients were associated with the severity of the case's complications and substantial risk of thrombosis. The concentration of oxysterols in the erythrocyte homogenate could be useful as a diagnostic biomarker to stand on the COVID-19 case severity.
Propagation and amplification law of undetermined stray light and influence of signal-to-noise ratio in a multipass amplifier system
Authors: Wang Zhenzhou , Xie Na , Huang Zeyu , Fu Xingqiao , Han Jinghua , Feng Guoying , Zhou Kainan , Su Jingqin
Published: January 2023

Multipass amplifier systems in areas such as high-energy-density physics and astrophysics play an important role, but the undetermined stray light caused by defects seriously influencing the output signal-to-noise ratio (SNR) and even resulting in the failure of the main pulse bombardment. In this study, the modulation of nondeterministic stray light caused by the defects in the optical paths of different components in a multipass amplifier system was examined, and a Mie scattering model of multi-crack defects established. Using ray-tracing simulation, the spatial and temporal distribution of stray light was analysed, and experimental verification was conducted. The law of energy amplification for nondeterministic stray light caused by component defect modulation and an empirical formula for the signal to noise ratio of the pre-pulse system were established. This study provides a reference for the accurate troubleshooting of components, optical path defects and an important theoretical basis for the processing and correction of system components.
We chased COVID-19; Did we forget Measles? - A machine learning approach to public discourse and sentiment analysis on spiking Measles cases
Authors: S. Anoop V. , Thekkiniath Jose
Published: January 2023

Objectives: This study employs text mining and natural language processing approaches for analyzing and unearthing public discourse and sentiment towards the recent spiking Measles outbreaks reported across the globe.
Study design: A detailed qualitative study was designed using text mining and natural language processing on the user-generated comments from Reddit, a social news aggregation and discussion website.
Methods: A detailed analysis was conducted using topic modeling and sentiment analysis on Reddit comments (n=87203) posted between October 1 and December 15, 2022. Topic modeling was used to leverage major themes related to the Measles health emergency and public discourse; the sentiment analysis was performed to check how the general public responded to different aspects of the outbreak.
Results: Our results revealed several intriguing and helpful themes, including parental concerns, anti-vaxxer discussions, and measles symptoms from the user-generated content. The results further confirm that even though there have been administrative interventions to promote vaccinations that affirm the parents' concerns to a greater extent, the anti-vaccination or vaccine hesitancy prevalent in the general public reduces the effect of such intercessions.
Conclusions: A proactive analysis of public discourse and sentiments during health emergencies and disease outbreaks is vital. This study effectively explored public perceptions and sentiments to assist health policy researchers and stakeholders in making informed and data-driven decisions.}
Importance of C3=C4 double bondof plant alkaloid Harmine on its binding interaction with Hemoglobin: Multi-spectroscopic and molecular modelling studies
Authors: Burman Mangal Deep , Bag Sagar , Bhowmik Sudipta
Published: January 2023

Harmine and harmaline are two structurally similar β-carboline plant alkaloids with various therapeutic properties, having difference in C3 = C4 double bond only. Nature of the interaction between hemoglobin (Hb) and harmine and harmaline was investigated in this study by employing different spectroscopic methods and molecular modeling. The spectrum of Hb showed hypochromic changes in the presence of both harmine and harmaline but with different efficiencies. The fluorescence quenching of harmine and harmaline occurred in the presence of Hb indicating stronger interation of harmine with Hb. Stoichiometry study revealed that both harmine and harmaline interact with Hb in around 1:1 ratio. The circular dichroism study demonstrate no conformational change of Hb in the presence of alkaloids. From the melting study, it was found that both harmine and harmaline do not affect the stability of Hb. Molecular modeling study supported the above findings that harmine containing the C3 = C4 double bond in its structure, interacts strongly with Hb on the surface of α2 chain compared to harmaline. This study helps us to understand that slight structural differences in harmine and harmaline can alter interaction properties significantly and these key informations may help in the drug discovery processes.
Genetic evidence for Colletotrichum gloeosporioides transmission between the invasive plant Ageratina adenophora and co-occurring neighbor plants
Authors: Zeng ZhaoYing , Yang ZhiPing , Yang AiLing , Li YuXuan , Zhang HanBo
Published: January 2023

To understand the disease-mediated invasion of exotic plants and the potential risk of disease transmission in local ecosystems, it is necessary to characterize population genetic structure and spatio-temporal dynamics of fungal community associated with both invasive and co-occurring plants. In this study, multiple genes were used to characterize the genetic diversity of 165 strains of Colletotrichum gloeosporioides species complex (CGSC) isolated from healthy leaves and symptomatic leaves of invasive plant A. adenophora, as well as from symptomatic leaves of its neighbor plants from 11 geographic sites in China. The data showed that these CGSC strains had a high genetic diversity in each geographic site (all Hd > 0.67 and Pi > 0.01). Haplotype diversity and nucleotide diversity varied greatly in individual gene locus: gs had the highest haplotype diversity (Hd = 0.8972), gapdh had the highest nucleotide diversity (Pi = 0.0705), and ITS had the lowest nucleotide diversity (Pi = 0.0074). Haplotypes were not clustered by geographic site, invasive age or isolation source. Both AMOVA and neutrality tests indicated these CGSC strains occurred gene exchange among geographic populations but did not experience population expansion along with A. adenophora invasion progress. Our data indicated that A. adenophora primarily accumulated these CGSC fungi in the introduced range, suggesting a high frequency of CGSC transmission between A. adenophora and co-occurring neighbor plants. This study is valuable for understanding the disease-mediated plant invasion and the potential risk of disease transmission driven by exotic plants in local ecosystems.
Wireless Subscribers with AODV Routing Protocol Simulated on Mobile Ad-hoc Networks utilized Ad-hoc on-Demand Distance Vector
Authors: BASIL NOORULDEN
Published: January 2023

Impromptu steering calculation has been a center issue in exploration of Ad-Hoc organizations. To manage different directing convention issues on an impromptu organization like unfortunate legitimacy and huge control above, this paper groups and thinks about specially appointed network steering conventions through the OPNET reproduction instrument and spotlights on testing two directing conventions' exhibition specifically, table-driven and on-request steering convention. The DSDV Destination Sequenced Distance Vector steering convention is the most utilized table-driven directing convention, while AODV (Ad Hoc On-Demand Distance Vector) and DSR (Dynamic source directing) directing conventions are the most utilized on request directing conventions. The presentation of these three conventions are recreated with an assortment of bundle conveyance proportion, normal start to finish delays, and directing burdens are likewise been broke down and looked at utilizing changed development speed (from 0 to 25 m/s) and a consistent bitrate source traffic model. The outcomes got from 20 organization hubs dispersed haphazardly showed that the on-request directing convention is the most ideal choice for a high portability climate.
WITHDRAWN: Trajectory Path Calculated for WiMAX Network Implemented on High Coverage Area
Authors: BASIL NOORULDEN
Published: January 2023

The Global Microwave Connection (WiMAX) is a remote standard 802.16 that gives rapid, move paces of 100 Mbps and an inclusion area of 50 km. Voice over Internet Protocol (VoIP) offers customers adaptable and minimal-expense IP communication. In any case, many difficulties stay in giving solid voice correspondence over the Internet. The presentation of a few boundaries, for example, multiband direct example and transfer speed in a star-course WiMAX organization, was assessed in a four-cell situation. Every cell has a versatile station and a base station. Network execution measurements, for example, transfer speed and MOS were utilized to assess the most productive VoIP codecs. Execution investigation was performed utilizing OPNET14.5. The outcomes utilizing the multiband channel (inactive) model were superior to the model (CSE for walkers A). Data transmission of 15 dB is roughly 1600 parcels each second, and − 1 dB is 1300 bundles each second. As per the information, the wiping out type multipath model had a preferable MOS esteem over the ITU type A passerby model.
Rocket Control system implementation with Cuckoo Optimization Algorithm
Authors: BASIL NOORULDEN
Published: January 2023

rocket control system with optimized cuckoo optimization algorithm has been investigated. In order to make control on this controlled rocket system that rely up on three coordination that known as pitch, roller, yaw respectively and can express it as the main parameters for any rocket in additional to that VR Sink has been added as viewpoint and connected with cuckoo optimization algorithm in order to get high controlling on rocket with comparison in cases between upper and lower bound, the rocket coordination axis has been optimized with this method belong to artificial intelligent algorithm. The gravity center on rocket depends on three axis that mentioned above, the rocket depend on directional that comes from optimized algorithm also to increase the work efficiency of controlled system and could be increase these coefficients to make gravity center work in a best performance and the implementation of rocket built using MATLAB Simulink.
Lactobacillus Paracasei L9 affects disease progression in Experimental Autoimmune Neuritis by regulating arginine metabolism
Authors: Meng Yuting , Mao Yu , Qiu Xiangjie , Tang Zhongxiang , Tan Yurong
Published: January 2023

Background: Autoimmune neuropathies are common peripheral nervous system (PNS) disorders. Environmental influences and dietary components are known to affect the course of autoimmune diseases. Intestinal microorganisms can be dynamically regulated through diet, and this study combines intestinal microorganisms with diseases to open up new therapeutic ideas.
Methods: In Lewis rats, a model of Experimental Autoimmune Neuritis（EAN）was established with P0 peptide, Lactobacillus were used as treatment, serum T-cell ratio, inflammatory factors, sciatic neuropathological changes, and pathological inflammatory effects on intestinal mucosa were detected, and fecal metabolomics and 16s microbiome analysis were performed to further explore the mechanism.
Results: In the EAN rat model, Lactobacillus paracasei L9 (LP) could dynamically regulate the CD4+/CD8+T balance in serum, reduce serum IL-1, IL-6 and TNF-α expression levels, improve sciatic nerve demyelination and inflammatory infiltration, and nervous system scores. Intestinal mucosa was damaged, Lactobacillus paracasei L9 (LP) gavage induced intestinal mucosa recovery; Occludin and ZO-1 upregulation; IL-1, TNF-α and Reg3γ downregulation. Finally, metabolomics and 16s microbiome analysis were performed, and differential metabolites were enriched with an important metabolic pathway, arginine and proline metabolism.
Conclusion: LP may improve EAN in rats by influencing intestinal community and the lysine and proline metabolism.
Emergency administration of fibrinogen concentrate for haemorrhage: systematic review and meta-analysis
Authors: Itagaki Yuki , Hayakawa Mineji , Takahashi Yuki , Hirano Satoshi , Yamakawa Kazuma
Published: January 2023

Introduction:
The occurrence of massive haemorrhages in various emergency situations increases the need for blood transfusions and increases the risk of mortality. Fibrinogen concentrate (FC) use may increase plasma fibrinogen levels more rapidly than fresh-frozen product or cryoprecipitate use. Previous several systematic reviews and meta-analyses have not effectively demonstrated FC efficacy in significantly improving the risk of mortality and reducing transfusion requirements. In this study, we investigated the use of FC for haemorrhages in emergency situations.
Methods and analysis:
In this systematic review and meta-analysis, we included controlled trials, but excluded randomized controlled trials (RCTs) in elective surgeries. The study population consisted of patients with haemorrhages in emergency situations, and the intervention was emergency supplementation of FC. The control group was administered with ordinal transfusion or placebo. The primary and secondary outcomes were in-hospital mortality and the amount of transfusion and thrombotic events, respectively. The electronic databases searched included MEDLINE (PubMed), Web of Science, and the Cochrane Central Register of Controlled Trials.
Results
Nine RCTs in the qualitative synthesis with a total of 701 patients were included. Results showed a slight increase in in-hospital mortality with FC treatment (RR 1.24, 95% CI: 0.64–2.39, p = 0.52) with very low certainty of the evidence. There was no reduction in the use of red blood cells (RBC) transfusion in the first 24 h after admission with FC treatment (mean difference [MD] 0.0 Unit in the FC group, 95% CI: -0.99–0.98, p = 0.99) with very low certainty of the evidence. However, the use of fresh frozen plasma (FFP) transfusion significantly increased in the first 24 h after admission with FC treatment (MD 2.61 Unit higher in the FC group, 95% CI: 0.07–5.16, p = 0.04). The occurrence of thrombotic events did not significantly differ with FC treatment.
Conclusions
The present study indicates that the use of FC may result in a slight increase in in-hospital mortality. While FC did not appear to reduce the use of RBC transfusion, it likely increased the use of FFP transfusion and may result in a large increase in platelet concentrate (PC) transfusion. However, the results should be interpreted cautiously due to the unbalanced severity in the patient population, high heterogeneity, and risk of bias.
Cs3Cu2I5 Single Crystal for Efficient Direct X-ray Detection
Authors: Qinhua Wei , Xiongsheng Fan , Peng Xiang , Laishun Qin , Wenjun Liu , Shi Tongyu , Hang Yin , Cai Peiqing , Yufeng Tong , Gao Tang , Liu Zugang , Chu Paul , Shi Hongsheng , Yanliang Liu , Yu Xue-Feng
Published: January 2023

Low-dimensional copper-based halide perovskite single crystals are considered excellent scintillators for indirect X-ray detection, but their potential in direct X-ray detection has not been investigated. Herein, high-quality pure Cs3Cu2I5 and Li-doped Cs3Cu2I5:Li single crystals are grown by the Bridgman method. The Li+ dopant enhances the photoelectric properties of the Cs3Cu2I5 single crystal by extending the carrier life time, improving the carrier mobility from 6.49 to 9.52 cm2V-1s-1, and increasing the mobility-lifetime (μτ) product from 1.4×10-4 to 2.9×10-4 cm2V–1. The sensitive direct X-ray detector with a vertical device configuration of Au/Cs3Cu2I5:Li single crystal/PCBM/Au is fabricated and demonstrated to have a high sensitivity of 831.1 µC Gyair -1 cm-2 and low detection limit of 34.8 nGyair s-1. Furthermore, the detector shows fast response, negligible baseline current drift and excellent stability upon X-ray illumination.