bluuebunny/crossref_metadata_2025 huggingface dataset:
AMG487 alleviates influenza A (H1N1) virus‐induced pulmonary inflammation through decreasing IFN‐γ‐producing lymphocytes and IFN‐γ concentrations
Authors: Ding Wenbin , Li Runfeng , Song Tongtong , Yang Zifeng , Xu Dongting , Huang Chuqin , Shen Shuirong , Zhong Nanshan , Lai Kefang , Deng Zheng
Published: March 2024

Background and Purpose

Severe influenza virus‐infected patients have high systemic levels of Th1 cytokines (including IFN‐γ). Intrapulmonary IFN‐γ increases pulmonary IFN‐γ‐producing T lymphocytes through the CXCR3 pathway. Virus‐infected mice lacking IP‐10/CXCR3 demonstrate lower pulmonary neutrophilic inflammation. AMG487, an IP‐10/CXCR3 antagonist, ameliorates virus‐induced lung injury in vivo through decreasing viral loads. This study examined whether AMG487 could treat H1N1 virus‐induced mouse illness through reducing viral loads or decreasing the number of lymphocytes or neutrophils.

Experimental Approach

Here, we studied the above‐mentioned effects and underlying mechanisms in vivo.

Key Results

H1N1 virus infection caused bad overall condition and pulmonary inflammation characterized by the infiltration of lymphocytes and neutrophils. From Day‐5 to Day‐10 post‐virus infection, bad overall condition, pulmonary lymphocytes, and IFN‐γ concentrations increased, while pulmonary H1N1 viral titres and neutrophils decreased. Both anti‐IFN‐γ and AMG487 alleviated virus infection‐induced bad overall condition and pulmonary lymphocytic inflammation. Pulmonary neutrophilic inflammation was mitigated by AMG487 on Day‐5 post‐infection, but was not mitigated by AMG487 on Day‐10 post‐infection. H1N1 virus induced increases of IFN‐γ, IP‐10, and IFN‐γ‐producing lymphocytes and activation of the Jak2‐Stat1 pathways in mouse lungs, which were inhibited by AMG487. Anti‐IFN‐γ decreased IFN‐γ and IFN‐γ‐producing lymphocytes on Day‐5 post‐infection. AMG487 but not anti‐IFN‐γ decreased viral titres in mouse lung homogenates or BALF. Higher virus load did not increase pulmonary inflammation and IFN‐γ concentrations when mice were treated with AMG487.

Conclusion and Implications

AMG487 may ameliorate H1N1 virus‐induced pulmonary inflammation through decreasing IFN‐γ‐producing lymphocytes rather than reducing viral loads or neutrophils.
Investigating student perceptions and vulnerability to heat stress in campus residences using Reddit: Climate change, health, and wellbeing
Authors: Yeung Yuki , Elliott Susan J.
Published: March 2024

This exploratory research investigated the sufficiency of existing infrastructure to adapt to high temperatures and explored the perceptions of heat stress from students in on‐campus residences at the U15 Group of Universities in Canada. The prevalence of air conditioning in student residences was used to estimate the adaptive capacity of existing infrastructure, and posts and comments on Reddit relevant to the perceptions of heat stress were collected in January 2023 through a query of relevant key words within each institution's subreddit. Most institutions (80%) had some residences with air conditioning. However, four main themes emerged through the thematic analysis of 409 posts and comments on Reddit: (1) complaints, (2) impacts on wellbeing, (3) adaptation strategies, and (4) climate change. The perceptions of heat stress from students suggest that existing available cooling strategies do not provide sufficient adaptation to high indoor temperatures. Recognizing student perceptions and experiences is necessary in designing and implementing future adaptation strategies to promote the health and wellbeing of postsecondary students in Canada.
Inducing Novel Sound–Taste Correspondences via an Associative Learning Task
Authors: Barbosa Escobar Francisco , Wang Qian Janice
Published: March 2024

The interest in crossmodal correspondences, including those involving sounds and involving tastes, has experienced rapid growth in recent years. However, the mechanisms underlying these correspondences are not well understood. In the present study (N = 302), we used an associative learning paradigm, based on previous literature using simple sounds with no consensual taste associations (i.e., square and triangle wave sounds at 200 Hz) and taste words (i.e., sweet and bitter), to test the influence of two potential mechanisms in establishing sound–taste correspondences and investigate whether either learning mechanism could give rise to new and long‐lasting associations. Specifically, we examined an emotional mediation account (i.e., using sad and happy emoji facial expressions) and a transitive path (i.e., sound‐taste correspondence being mediated by color, using red and black colored squares). The results revealed that the associative learning paradigm mapping the triangle wave tone with a happy emoji facial expression induced a novel crossmodal correspondence between this sound and the word sweet. Importantly, we found that this novel association was still present two months after the experimental learning paradigm. None of the other mappings, emotional or transitive, gave rise to any significant associations between sound and taste. These findings provide evidence that new crossmodal correspondences between sounds and tastes can be created by leveraging the affective connection between both dimensions, helping elucidate the mechanisms underlying these associations. Moreover, these findings reveal that these associations can last for several weeks after the experimental session through which they were induced.
FVCNet: Detection obstacle method based on feature visual clustering network in power line inspection
Authors: Wang Qiu‐Yu , Lv Xian‐Long , Tang Shi‐Kai
Published: March 2024

Power line inspection is an important means to eliminate hidden dangers of power lines. It is a difficult research problem how to solve the low accuracy of power line inspection based on deep neural network (DNN) due to the problems of multi‐view‐shape, small‐size object. In this paper, an automatic detection model based on Feature visual clustering network (FVCNet) for power line inspection is established. First, an unsupervised clustering method for power line inspection is proposed, and applied to construct a detection model which can recognize multi‐view‐shape objects and enhanced object features. Then, the bilinear interpolation method is used to Feature enhancement method, and the enhanced high‐level semantics and low‐level semantics are fused to solve the problems of small object size and single sample. In this paper, FVCNet is applied to the MS‐COCO 2017 data set and self‐made power line inspection data set, and the test accuracy is increased to 61.2% and 82.0%, respectively. Compared with other models, especially for the categories that are greatly affected by multi‐view‐shape, the test accuracy has been improved significantly.
An effective graph embedded YOLOv5 model for forest fire detection
Authors: Yuan Hui , Lu Zhumao , Zhang Ruizhe , Li Jinsong , Wang Shuai , Fan Jingjing
Published: March 2024

The existing YOLOv5‐based framework has achieved great success in the field of target detection. However, in forest fire detection tasks, there are few high‐quality forest fire images available, and the performance of the YOLO model has suffered a serious decline in detecting small‐scale forest fires. Making full use of context information can effectively improve the performance of small target detection. To this end, this paper proposes a new graph‐embedded YOLOv5 forest fire detection framework, which can improve the performance of small‐scale forest fire detection using different scales of context information. To mine local context information, we design a spatial graph convolution operation based on the message passing neural network (MPNN) mechanism. To utilize global context information, we introduce a multi‐head self‐attention (MSA) module before each YOLO head. The experimental results on FLAME and our self‐built fire dataset show that our proposed model improves the accuracy of small‐scale forest fire detection. The proposed model achieves high performance in real‐time performance by fully utilizing the advantages of the YOLOv5 framework.
Aminobenzotriazole inhibits and induces several key drug metabolizing enzymes complicating its utility as a pan CYP inhibitor for reaction phenotyping
Authors: Aluri Krishna C. , Slavsky Marina , Tan Ying , Whitcher‐Johnstone Andrea , Zhang Zhoupeng , Hariparsad Niresh , Ramsden Diane
Published: March 2024

Aminobenzotriazole (ABT) is commonly used as a non‐selective inhibitor of cytochrome P450 (CYP) enzymes to assign contributions of CYP versus non‐CYP pathways to the metabolism of new chemical entities. Despite widespread use, a systematic review of the drug–drug interaction (DDI) potential for ABT has not been published nor have the implications for using it in plated hepatocyte models for low clearance reaction phenotyping. The goal being to investigate the utility of ABT as a pan‐CYP inhibitor for reaction phenotyping of low clearance compounds by evaluating stability over the incubation period, inhibition potential against UGT and sulfotransferase enzymes, and interaction with nuclear receptors involved in the regulation of drug metabolizing enzymes and transporters. Induction potential for additional inhibitors used to ascribe fraction metabolism (fm), pathway including erythromycin, ketoconazole, azamulin, atipamezole, ZY12201, and quinidine was also investigated. ABT significantly inhibited the clearance of a non‐selective UGT substrate 4‐methylumbelliferone, with several UGTs shown to be inhibited using selective probe substrates in human hepatocytes and rUGTs. The inhibitors screened in the induction assay were shown to induce enzymes regulated through Aryl Hydrocarbon Receptor, Constitutive Androstane Receptor, and Pregnane X Receptor. Lastly, a case study identifying the mechanisms of a clinical DDI between Palbociclib and ARV‐471 is provided as an example of the potential consequences of using ABT to derive fm. This work demonstrates that ABT is not an ideal pan‐CYP inhibitor for reaction phenotyping of low clearance compounds and establishes a workflow that can be used to enable robust characterization of other prospective inhibitors.
Estimation of the benefit from pre‐emptive genotyping based on the nationwide cohort data in South Korea
Authors: Huh Ki Young , Hwang Sejung , Na Joo Young , Yu Kyung‐Sang , Jang In‐Jin , Chung Jae‐Yong , Yoon Seonghae
Published: March 2024

Genetic variants affect drug responses, making pre‐emptive genotyping crucial for averting serious adverse events (SAEs) and treatment failure. However, assessing the benefits of pre‐emptive genotyping based on genetic distribution, drug exposure, and demographics is challenging. This study aimed to estimate the population‐level benefits of pre‐emptive genotyping in the Korean population using nationwide cohort data. We reviewed actionable gene‐drug combinations recommended by both the Clinical Pharmacogenomics Implementation Consortium (CPIC) and the Dutch Pharmacogenetics Working Group (DPWG) as of February 2022, identifying high‐risk phenotypes. We collected reported risk reduction from genotyping and standardized it into population attributable risks. Healthcare reimbursement costs for SAEs and treatment failures were obtained from the Health Insurance Review and Assessment Service Statistics in 2021. The benefits of pre‐emptive genotyping for a specific group were determined by multiplying drug exposure from nationwide cohort data by individual genotyping benefits. We identified 31 gene‐drug‐event pairs, with CYP2D6 and CYP2C19 demonstrating the greatest benefits for both male and female patients. Individuals aged 65–70 years had the highest individual benefit from pre‐emptive genotyping, with $84.40 for men and $100.90 for women. Pre‐emptive genotyping, particularly for CYP2D6 and CYP2C19, can provide substantial benefits.
Efficient species identification for Pacific salmon genetic monitoring programs
Authors: Robinson Zachary L. , Stephenson Jeff , Vertacnik Kim , Willis Stuart , Horn Rebekah , McCane Jesse , Coykendall D. Katharine , Narum Shawn R.
Published: March 2024

Genetic monitoring of Pacific salmon in the Columbia River basin provides crucial information to fisheries managers that is otherwise challenging to obtain using traditional methods. Monitoring programs such as genetic stock identification (GSI) and parentage‐based tagging (PBT) involve genotyping tens of thousands of individuals annually. Although rare, these large sample collections inevitably include misidentified species, which exhibit low genotyping success on species‐specific Genotyping‐in‐Thousands by sequencing (GT‐seq) panels. For laboratories involved in large‐scale genotyping efforts, diagnosing non‐target species and reassigning them to the appropriate monitoring program can be costly and time‐consuming. To address this problem, we identified 19 primer pairs that exhibit consistent cross‐species amplification among salmonids and contain 51 species informative variants. These genetic markers reliably discriminate among 11 salmonid species and two subspecies of Cutthroat Trout and have been included in species‐specific GT‐seq panels for Chinook Salmon, Coho Salmon, Sockeye Salmon, and Rainbow Trout commonly used for Pacific salmon genetic monitoring. The majority of species‐informative amplicons (16) were newly identified from the four existing GT‐seq panels, thus demonstrating a low‐cost approach to species identification when using targeted sequencing methods. A species‐calling script was developed that is tailored for routine GT‐seq genotyping pipelines and automates the identification of non‐target species. Following extensive testing with empirical and simulated data, we demonstrated that the genetic markers and accompanying script accurately identified species and are robust to missing genotypic data and low‐frequency, shared polymorphisms among species. Finally, we used these tools to identify Coho Salmon incidentally caught in the Columbia River Chinook Salmon sport fishery and used PBT to determine their hatchery of origin. These molecular and computing resources provide a valuable tool for Pacific salmon conservation in the Columbia River basin and demonstrate a cost‐effective approach to species identification for genetic monitoring programs.
An efficient multi‐scale transformer for satellite image dehazing
Authors: Yang Lei , Cao Jianzhong , Chen Weining , Wang Hao , He Lang
Published: March 2024

Given the impressive achievement of convolutional neural networks (CNNs) in grasping image priors from extensive datasets, they have been widely utilized for tasks related to image restoration. Recently, there is been significant progress in another category of neural architectures—Transformers. These models have demonstrated remarkable performance in natural language tasks and higher‐level vision applications. Despite their ability to address some of CNNs limitations, such as restricted receptive fields and adaptability issues, Transformer models often face difficulties when processing images with a high level of detail. This is because the complexity of the computations required increases significantly with the image's spatial resolution. As a result, their application to most high‐resolution image restoration tasks becomes impractical. In our research, we introduce a novel Transformer model, named DehFormer, by implementing specific design modifications in its fundamental components, for example, the multi‐head attention and feed‐forward network. Specifically, the proposed architecture consists of the three modules, that is, (a) multi‐scale feature aggregation network (MSFAN), (b) the gated‐Dconv feed‐forward network (GFFN), (c) and the multi‐Dconv head transposed attention (MDHTA). For the MDHTA module, our objective is to scrutinize the mechanics of scaled dot‐product attention through the utilization of per‐element product operations, thereby bypassing the need for matrix multiplications and operating directly in the frequency domain for enhanced efficiency. For the GFFN module, which enables only the relevant and valuable information to advance through the network hierarchy, thereby enhancing the efficiency of information flow within the model. Extensive experiments are conducted on the SateHazelk, RS‐Haze, and RSID datasets, resulting in performance that significantly exceeds that of existing methods.
How developing a point of need training tool for evidence synthesis can improve librarian support for researchers
Authors: Chiang Bronte , McClurg Caitlin
Published: March 2024

Medical and health sciences librarians who are involved in evidence synthesis projects will know that systematic reviews are intensely rigorous, requiring research teams to devote significant resources to the methodological process. As expert searchers, librarians are often identified as personnel to conduct the database searching portion and/or are approached as experts in the methodology to guide research teams through the lifecycle of the project. This research method has surged in popularity at our campus and demand for librarian participation is unsustainable. As a response to this, the library created self‐directed learning objects in the form of roadmap to assist researchers in learning about the knowledge synthesis methodology in an expedient, self‐directed manner. This paper will discuss the creation, implementation and feedback around our educational offering: Systematic & Scoping Reviews: Your Roadmap to Conducting an Evidence Synthesis.
The cascade of care for hepatitis C in Victoria, Australia: a data linkage cohort study
Authors: Snow Kathryn , MacLachlan Jennifer H. , Rowe Stacey , Higgins Nasra , Cowie Benjamin C.
Published: March 2024

Background

Highly effective hepatitis C therapies are available in Australia. However, people living with hepatitis C face various barriers to accessing care and treatment.

Aims

To identify gaps in the cascade of care for hepatitis C and generate estimates of the number living with untreated infection according to population group, using a representative longitudinal study population.

Methods

We linked hepatitis C notification data from Victoria to national pathology, prescribing and death registry data. We assessed receipt of key clinical services in a large cohort who tested positive for hepatitis C from 1 January 2000 to 31 December 2016, with follow‐up to 30 June 2018. We estimated the number still living with hepatitis C, adjusting for spontaneous clearance and mortality.

Results

The cohort comprised 45 391 people positive for hepatitis C. Of these, 13 346 (29%) received treatment and an estimated 28% (95% confidence interval (CI): 26–30%) were still living with chronic infection at 30 June 2018, with the remainder still living following spontaneous clearance (30%, 95% CI: 29–32%) or having died (12%, 95% CI: 12–12%). Half (50%) of those still living with hepatitis C were born from 1965 to 1980, and 74% first tested positive before 2011.

Conclusions

Despite an enabling policy environment and subsidised therapy, many people in this cohort were not treated. Increased measures may be needed to engage people in care, including those who acquired hepatitis C more than 10 years ago.
Pathways and transitions for patients admitted to an emergency department after self‐harming events
Authors: Sandahl Malene , Lassen Annmarie Touborg , Stenager Elsebeth , Østervang Christina
Published: March 2024

The frequency of people presented in emergency departments (EDs) after self‐harming events is increasing. Previous studies have shown that the complexity of the disorders of patients admitted to the ED after self‐harming events can be overwhelming for ED healthcare professionals (HCPs) to handle. The objective of this study was to observe and investigate the pathways for patients admitted to the ED after self‐harming events to either transition or discharge. Participant observation and interviews were selected as the methods to generate insight into the pathways of patients admitted to the ED after self‐harming events. The data were analysed using interpretative phenomenological analysis. A sample size of 20 patients was analysed, and a total of 213 h of observation took place during the data collection. Three main themes appeared: (1) patients' mental stress versus high expectations, (2) uncertainty about how to address the self‐harming event and (3) a system of chaos. Patients admitted to the ED after self‐harming events struggle with difficult mental stress. Despite this, they face high expectations that they will fit in and cooperate in the ED. The healthcare system is organised with unclear responsibilities and without systematic ways to care for self‐harm patients and so provides chaotic patient pathways. There is a need for improved cross‐sectional competencies, mutual agreements and systematic communication for discharge, transitions and follow‐up care between those involved in the patient's pathway and care.
Patterns of Non‐influenza Respiratory Viruses Among Severe Acute Respiratory Infection Cases in Burkina Faso: A Surveillance Study
Authors: Lingani Moussa , Cissé Assana , Ilboudo Abdoul Kader , Yaméogo Issaka , Tarnagada Zekiba
Published: March 2024

Background

Although influenza viruses cause only one‐fifth of severe acute respiratory infections (SARI) in Burkina Faso, the other viral causes of SARI remain poorly investigated to inform clinical and preventive decision making.

Methods

Between 2016 and 2019, we prospectively enrolled inpatients meeting the World Health Organization (WHO) case definition of SARI in Burkina Faso. Results of viral etiologies among inpatients tested negative for influenza using the Fast Track Diagnostics Respiratory Kits (FTD‐33) were reported.

Results

Of 1541 specimens tested, at least one respiratory virus was detected in 76.1% of the 1231 specimens negative for influenza virus. Human rhinoviruses (hRVs) were the most detected pathogens (476; 38.7%), followed by human adenoviruses (hAdV) (17.1%, 210/1231), human respiratory syncytial virus (hRSV) (15.4%, 189/1231), enterovirus (EnV) (11.2%, 138/1231), human bocavirus (hBoV) (7.9%, 97/1231), parainfluenza 3 (hPIV3) (6.1%, 75/1231), human metapneumovirus (hMPV) (6.0%,74/1321), parainfluenza 4 (hPIV4) (4.1%, 51/1231), human coronavirus OC43 (hCoV‐OC43) (3.4%, 42/1231), human coronavirus HKU1(hCoV‐HKU1) (2.7%, 33/1231), human coronavirus NL63 (hCoV‐NL63) (2.5%, 31/1231), parainfluenza 1 (hPIV1) (2.0%, 25/1231), parainfluenza 2 (hPIV2) (1.8%, 22/1231), human parechovirus (PeV) (1.1%, 14/1231), and human coronavirus 229E (hCoV‐229E) (0.9%, 11/1231). Among SARI cases, infants aged 1–4 years were mostly affected (50.7%; 622/1231), followed by those <1 year of age (35.7%; 438/1231). Most detected pathogens had year‐long circulation patterns, with seasonal peaks mainly observed during the cold and dry seasons.

Conclusion

Several non‐influenza viruses are cause of SARI in Burkina Faso. The integration of the most common pathogens into the routine influenza surveillance system might be beneficial.
Life‐history traits of an exploited skate: The short‐tail yellownose skate Zearaja brevicaudata (Chondrichthyes: Rajiformes)
Authors: Bianchi Santiago J. , Roman Jorge M. , Lucifora Luis O. , Barbini Santiago A.
Published: March 2024

The short‐tail yellownose skate, Zearaja brevicaudata, occurs off southern Brazil and along the Argentine continental shelf, including waters surrounding the Islas Malvinas and the upper slope, and is very frequently caught by trawl fisheries throughout its range. The age, growth, and maturity of Z. brevicaudata were investigated using 204 individuals collected by commercial fishing vessels from northern Argentina, between March 2016 and September 2019. Age was determined on a sample comprising vertebrae from 151 individuals, 60 males, ranging in size from 391 to 956 mm total length (TL), and 91 females, ranging in size from 324 to 1060 mm TL. Maximum ages determined for males and females were 22 and 29 years, respectively. A Bayesian framework was employed with a set of three candidate models to estimate growth parameters (von Bertalanffy, Gompertz, and Logistic). The von Bertalanffy model had the best fit, and the sexes showed undistinguishable growth parameters. For sexes combined, derived growth mean parameters (± S.D.) were L∞ = 1081 mm ± 64.34 mm, k = 0.09 ± 0.01 years−1, and L0 = 248 mm ± 23.52 mm. The age at maturity was estimated at 13.15 and 14.66 years for males and females, respectively.
Shortcuts and detours of environmental collective legal mobilizations: the cases of the Atrato River and the Amazon region in Colombia
Authors: CIESIELSKI MARKUS , GARCÍA CARVAJAL CARLOS ANDRÉS , VARGAS TRUJILLO JULIETTE
Published: March 2024

Colombia's Atrato River and Amazon region were declared legal entities by Colombian judges in 2016 and 2018 respectively. This set the stage for a new era of environmental litigation in Colombia, in which a number of natural entities have been granted this status. In both contrasting cases, non‐governmental organizations (NGOs) brought dramatic environmental harms to court through the special acción de tutela procedure, but did not claim a new ecocentric legal status for nature. This article asks how rights were collectively mobilized in the Atrato and Amazon cases and led to ecocentric judgments. Semi‐structured interviews were conducted in 2020 with members of NGOs and grassroots organizations involved in these two cases, revealing the emergence of grievance collectives. Through the analysis of these qualitative data, we argue for a perspective that considers the interdependence of legal mobilization and the collective as a social relationship. Collective support led to the choice of an unconventional process. However, this supported the litigation collectives and resulted in ecocentric judgments that generated challenges for the associated collectives in the post‐litigation phase. Thus, the analysis of legal mobilization and social relations in the collectives as interdependent improves the understanding of the shortcuts and detours through which complex conflicts have been presented in cases of environmental litigation, and helps to strengthen the scope and concepts of collective legal mobilization.
The association between well‐being and olfactory sensitivity
Authors: Joshi Akshita , Hummel Thomas
Published: March 2024

Well‐being (WB) is defined as a healthy state of mind and body. It consists of mental, physical, and social domains. In our everyday life, we perceive millions of odors from our environment, which have a role in social communication, protecting us from the environmental hazards and in our quality of life. A lot is known about the association between olfaction and depression; however, the relation between WB and olfaction remains unexplored. In the study, we reanalyzed 122 healthy subjects (mean age of 26 ± 3 years; 58 men/64 women). WB state was examined using the 42‐item, General habitual well‐being questionnaire and olfactory sensitivity was assessed using the Sniffin' Sticks test. We found out a significant positive correlation between the WB and olfactory sensitivity r119 = .21, p = .023. Therefore, it is evident that even in healthy subjects, there exist a positive relationship between the sense of smell and the WB state. This connection may offer valuable insights into potential indicators of future mental health conditions.

Practical Applications

The sense of smell plays a critical role in establishing social connections, including kin recognition and the development of sexual relationships. Olfactory loss serves as an early indicator for depression. Likewise, in individuals without apparent health issues, assessing smell function can serve as a potential precursor for identifying mental health conditions. Consequently, it is advisable to integrate smell testing into routine medical simulation training.
Blood gases, acid‐base, and metabolic alterations in calves with bronchopneumonia diagnosed via clinical signs and thoracic ultrasonography: A cross‐sectional study
Authors: Boccardo Antonio , Ferrulli Vincenzo , Sala Giulia , Scavone Donatella , Paltrinieri Saverio , Filippone Pavesi Laura , Pravettoni Davide
Published: March 2024

Background

Bronchopneumonia (BP) in calves potentially causes systemic changes.

Objectives

To describe metabolic, arterial blood gas, and acid‐base disorders in calves with BP diagnosed by thoracic ultrasound (TUS), Wisconsin score (WISC), and combinations of WISC and TUS.

Animals

Two hundred thirty‐one dairy preweaned dairy calves from 13 dairy farms.

Methods

Cross‐sectional study. Each calf sequentially underwent arterial blood gas evaluation, WISC score, venous sampling, and TUS. Calves were grouped based on a single diagnostic method and combination of WISC and 2 TUS cutoffs (≥1 cm; ≥3 cm) as healthy, upper respiratory tract infection, subclinical BP, and clinical BP.

Results

Oxygenation and acid‐base variables were unaffected. Glucose concentration in TUS‐affected calves was significantly lower (P < .001) than in healthy calves (median ≥TUS1cm = 5.2 mmol/L 25%‐75% interquartile range [IQR] 4.5‐6.1, <TUS1cm = 5.9 mmol/L IQR 5.5‐6.6; ≥TUS3cm = 5.1 mmol/L IQR 4.4‐6.2, <TUS3cm = 5.8 mmol/L IQR 5.3‐6.4). Paraoxonase‐1 was significantly lower (P < .001) in TUS‐affected calves (≥TUS1cm = 64.1 U/mL IQR 40.8‐78, <TUS1cm = 77.3 U/mL IQR 61.9‐96.5; ≥TUS3cm = 59.2 U/mL IQR 37.7‐72.4, <TUS3cm = 72.9 U/mL IQR 53.4‐95.5). None of the variables highlighted clear distinctions in WISC‐detected clinical and subclinical BP calves based on the combination of WISC and TUS.

Conclusions and Clinical Importance

Clinical signs indicate minor systemic disorders compared to TUS. The abnormalities detected by ultrasonographic examination were moderate and did not deviate from normal reference ranges.
Shovel point optimization for unmanned loader based on pile reconstruction
Authors: Chen Guanlong , Wang Yakun , Li Xue , Bi Qiushi , Li Xuefei
Published: March 2024

This study details an advanced shovel point optimization system for unmanned loaders, crucial for efficient shovelling operations. First, the shovel point evaluation index is established with reference to the driver's experience. Second, a novel method for pile profile reconstruction is proposed, utilizing a trained neural network to detect piles and extracting the point cloud using LiDAR and camera fusion. Subsequently, the system employs optimization algorithm to identify the best shovel point. Finally, 62 consecutive working experiments are successfully conducted. The system's performance closely approximates the driver's choices and achieves an average bucket fill factor of 97.7% for four materials. Results demonstrate the proposed method is reliable and efficient and contributes to the development of automated construction machinery.
The effect and mechanism of liraglutide on the biological functions of BMSCs in diabetic patients
Authors: Wang Yao , Sun Yujie , Yang Bo , Li Jun , Liang Chao
Published: March 2024

Objectives

To investigate the effect of liraglutide on osteogenesis in human alveolar bone marrow mesenchymal stem cells (BMSCs) and the influence of liraglutide on implant–bone integration in rats with T2DM.

Subjects and methods

Extracting BMSCs from the alveoli of diabetic patients treated with insulin. BMSCs were treated with different concentrations of liraglutide. Osteogenesis and the underlying mechanism were investigated via ALP detection, ALP staining, Alizarin Red S staining, Western blotting, and RT‐PCR. Liraglutide was given to Wistar and GK rats after implantation, and new bone formation around the implants was analyzed via micro‐CT. Implant–bone integration in rats was investigated via toluidine blue staining.

Results

Liraglutide enhanced osteogenesis in BMSCs via the BMP2/Smad/Runx2 signaling pathway. The optimal concentration of liraglutide that promoted osteogenesis was 10−8 mol/L. At concentrations higher than 10−7 mol/L, liraglutide had a negative effect on BMSCs. At a concentration of 10−8 mol/L liraglutide, BMSCs and diabetes mellitus–bone marrow stromal cells (DM‐BMSCs) showed optimal osteogenesis. Liraglutide promoted implant–bone integration and new bone formation in Wistar and GK rats.

Conclusions

Liraglutide not only promotes osteogenesis of BMSCs in normoglycemic individuals but also enhances osteogenesis of BMSCs in diabetic patients treated with insulin and enhances osseointegration in rats.
Tilletia horrida glycoside hydrolase family 128 protein, designated ThGhd_7, modulates plant immunity by blocking reactive oxygen species production
Authors: Shu Xinyue , Yin Desuo , Liang Juan , Xiang Ting , Zhang Chao , Li Honglian , Zheng Aiping , Li Ping , Wang Aijun
Published: March 2024

Tilletia horrida is an important soilborne fungal pathogen that causes rice kernel smut worldwide. We found a glycoside hydrolase family 128 protein, designated ThGhd_7, caused cell death in Nicotiana benthamiana leaves. The predicted signal peptide (SP) of ThGhd_7 targets it for secretion. However, loss of the SP did not affect its ability to induce cell death. The 23–201 amino acid sequence of ThGhd_7 was sufficient to trigger cell death in N. benthamiana. ThGhd_7 expression was induced and upregulated during T. horrida infection. ThGhd_7 localised to both the cytoplasm and nucleus of plant cells, and nuclear localisation was required to induce cell death. The ability of ThGhd_7 to trigger cell death in N. benthamiana depends on RAR1 (required for Mla12 resistance), SGT1 (suppressor of G2 allele of Skp1), and BAK1/SERK3 (somatic embryogenesis receptor‐like kinase 3). Heterologous overexpression of ThGhd_7 in rice reduced reactive oxygen species (ROS) production and enhanced susceptibility to T. horrida. Further research revealed that ThGhd_7 interacted with and destabilised OsSGT1, which is required for ROS production and is a positive regulator of rice resistance to T. horrida. Taken together, these findings suggest that T. horrida employs ThGhd_7 to disrupt ROS production and thereby promote infection.
The roots of Euroscepticism: Affective, behavioural and cognitive anti‐EU attitudes in Hungary
Authors: Bíró‐Nagy András , Szászi Áron József
Published: March 2024

This paper has put forward a new indicator based on the “ABC‐model” of attitudes designed to capture individual‐level Euroscepticism. We constructed a composite anti‐EU score based on affective, behavioural and cognitive sub‐indices. The study analyses data from a representative, countrywide Hungarian public opinion research. Looking for the drivers of anti‐EU attitudes, we took an integrative approach linking various theories of voting behaviour, Euroscepticism and populism studies. Our research showed that subjective well‐being significantly reduces anti‐EU attitudes. We found some empirical support for the “left behind thesis” in the Hungarian context, as severe economic grievances and fear of losing social status explained anti‐EU attitudes. Authoritarian traits turned out to be a major driver of Euroscepticism in Hungary. We proved that cosmopolitan values reduce affective and cognitive anti‐EU attitudes, but foreign experience and some international ties significantly correlated with anti‐EU stance. We confirmed that being a supporter of the governing Fidesz party and sharing culturally right‐wing political views significantly increase anti‐EU attitudes. We also revealed that partisanship's effect is strengthened by affective polarization and political interest.
Reimagining Theological Education in an Interreligious Setting: A Hindu Perspective
Authors: Rodrigues Pravina
Published: March 2024

Hindu sacred scriptures are a rich interweave of cosmological revelation that is embedded in melopoeia (melodic poetry) and sung in designated meters during rituals, liturgical services, festivals, and personal prayers. Revelation is conveyed through enigmatic dialogues, debates, parables, anecdotes, legends, and narratives. These occur between mendicants and saints, kings and mystics, and sometimes fables that include the natural world. These narrative accounts appeal to the young and the old and influence the body–mind–sense complex. A growing body of evidence attests to the positive effects of music and storytelling in the classroom setting. Drawing from these data and Hindu understandings of sacred mantric sound and storytelling, I discuss my experiments with musical frameworks and storytelling in my classes at the Graduate Theological Union, the Jesuit School of Theology of Santa Clara University, and Starr King School for the Ministry in California. I contend that the incorporation of parables, tales, legends, and narratives within a musical melodic framework, as is used in Hindu texts, is an effective means of imparting Hindu–Christian theological education.
Cardiac output‐mediated regulation of cerebral blood flow during exercise: Clinical perspectives on the indirect impact of muscle metaboreflex
Authors: Ogoh Shigehiko
Published: March 2024

The muscle metaboreflex stimulates the elevation of arterial blood pressure, aiming to rectify the oxygen deficit by enhancing oxygen delivery to support muscle activity. Moreover, activating the muscle metaboreflex significantly increases cardiac output (CO) by increasing factors such as heart rate, ventricular contractility, preload, stroke volume and mobilization of central blood volume. Previous studies indicate that ageing and cardiovascular diseases modify the muscle metaboreflex during exercise, limiting the ability to increase CO during physical activity. Alongside reduced exercise capacity, the attenuated rise in CO due to abnormal muscle metaboreflex in these patients impedes the increase in cerebral blood flow during exercise. Considering that CO plays a pivotal role in regulating cerebral blood flow adequately during exercise, this occurrence might contribute to an elevated risk of cerebral diseases, and it could also, at least, reduce the effective role of exercise in preventing cerebral disease and dementia among elderly individuals and patients with cardiovascular conditions. Therefore, it is important to consider this phenomenon when optimizing the effectiveness of exercise rehabilitation in patients with cardiovascular disease to prevent cerebral diseases and dementia.
Antagonism of 5‐HT2 receptors attenuates self‐sustained firing of human motor units
Authors: Goodlich Benjamin I. , Pearcey Gregory E. P. , Del Vecchio Alessandro , Horan Sean A. , Kavanagh Justin J.
Published: March 2024

5‐HT2 receptors on motoneurones play a critical role in facilitating persistent inward currents (PICs). Although facilitation of PICs can enhance self‐sustained firing after periods of excitation, the relationship between 5‐HT2 receptor activity and self‐sustained firing in human motor units (MUs) has not been resolved. MU activity was assessed from the tibialis anterior of 10 healthy adults (24.9 ± 2.8 years) during two contraction protocols. Both protocols featured steady‐state isometric contractions with constant descending drive to the motoneurone pool. However, one protocol also included an additional phase of superimposed descending drive. Adding and then removing descending drive in the middle of steady‐state contractions altered MU firing behaviour across the motor pool, where newly recruited units in the superimposed phase were unable to switch off (P = 0.0002), and units recruited prior to additional descending drive reduced their discharge rates (P < 0.0001, difference in estimated marginal means (∆) = 2.24 pulses/s). The 5‐HT2 receptor antagonist, cyproheptadine, was then administered to determine whether changes in MU firing were mediated by serotonergic mechanisms. 5‐HT2 receptor antagonism caused reductions in MU discharge rate (P < 0.001, ∆ = 1.65 pulses/s), recruitment threshold (P = 0.00112, ∆ = 1.09% maximal voluntary contraction) and self‐sustained firing duration (P < 0.0001, ∆ = 1.77s) after the additional descending drive was removed in the middle of the steady‐state contraction. These findings indicate that serotonergic neuromodulation plays a key role in facilitating discharge and self‐sustained firing of human motoneurones, where adaptive changes in MU recruitment must occur to meet the demands of the contraction.

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Key points

Animal and cellular preparations indicate that somato‐dendritic 5‐HT2 receptors regulate the intrinsic excitability of motoneurones.

5‐HT2 receptor antagonism reduces estimates of persistent inward currents in motoneurones, which contribute to self‐sustained firing when synaptic inputs are reduced or removed.

This human study employed a contraction task that slowly increased (and then removed) the additional descending drive in the middle of a steady‐state contraction where marked self‐sustained firing occurred when the descending drive was removed.

5‐HT2 receptor antagonism caused widespread reductions in motor unit (MU) discharge rates during contractions, which was accompanied by reduced recruitment threshold and attenuation of self‐sustained firing duration after the removal of the additional descending drive to motoneurones.

These findings support the role that serotonergic neuromodulation is a key facilitator of MU discharge and self‐sustained firing of human motoneurones, where adaptative changes in MU recruitment must occur to meet the demands of the contraction.
A New Typology of Out-of-School Youth Sports in 21st Century America: The Contrasting Organizational Logics of “Sport-Focused” and “Sport-for-Development” Programming Under Neoliberal Conditions
Authors: Hartmann Douglas , Swartz Teresa Toguchi , Campos Edgar Jesus , August Amy , Manning Alex , Billups Sarah Catherine
Published: March 2024

Out-of-school youth sport in the United States is bigger, more varied, and more impactful than ever before. In dialogue with existing scholarship, this paper uses multisite, collaborative fieldwork to identify core elements of program variation and develop a composite typology of this organizational field. The typology is based on a distinction between “sport-focused” programs and programs oriented toward nonsport social and developmental goals. Our primary insight is that programs within these domains exhibit two different organizational logics, one hierarchical, the other categorical. We also argue that variabilities of funding, social context, and reliance on public facilities are additional factors that impact the operation and effectiveness of these program types including their ability to address the racialized challenges of access, equity, and inclusion. Theorizing these differential configurations and their underlying characteristics can help parents, policymakers, practitioners (including coaches), and sports researchers engage youth sports more effectively under increasingly competitive neoliberal conditions.
Longitudinal intravital imaging of mouse placenta
Authors: Zhu Xiaoyi , Huang Qiang , Jiang Laiming , Nguyen Van-Tu , Vu Tri , Devlin Garth , Shaima Jabbar , Wang Xiaobei , Chen Yong , Ma Lijun , Xiang Kun , Wang Ergang , Rong Qiangzhou , Zhou Qifa , Kang Yubin , Asokan Aravind , Feng Liping , Hsu Shiao-Wen D. , Shen Xiling , Yao Junjie
Published: March 2024

Studying placental functions is crucial for understanding pregnancy complications. However, imaging placenta is challenging due to its depth, volume, and motion distortions. In this study, we have developed an implantable placenta window in mice that enables high-resolution photoacoustic and fluorescence imaging of placental development throughout the pregnancy. The placenta window exhibits excellent transparency for light and sound. By combining the placenta window with ultrafast functional photoacoustic microscopy, we were able to investigate the placental development during the entire mouse pregnancy, providing unprecedented spatiotemporal details. Consequently, we examined the acute responses of the placenta to alcohol consumption and cardiac arrest, as well as chronic abnormalities in an inflammation model. We have also observed viral gene delivery at the single-cell level and chemical diffusion through the placenta by using fluorescence imaging. Our results demonstrate that intravital imaging through the placenta window can be a powerful tool for studying placenta functions and understanding the placental origins of adverse pregnancy outcomes.
Functional dissection of the spike glycoprotein S1 subunit and identification of cellular cofactors for regulation of swine acute diarrhea syndrome coronavirus entry
Authors: Yang Yong-Le , Wang Bin , Li Wentao , Cai Hou-Li , Qian Qian-Yu , Qin Yu , Shi Fang-Shu , Bosch Berend-Jan , Huang Yao-Wei
Published: March 2024

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel porcine enteric coronavirus, and the broad interspecies infection of SADS-CoV poses a potential threat to human health. This study provides experimental evidence to dissect the roles of distinct domains within the SADS-CoV spike S1 subunit in cellular entry. Specifically, we expressed the S1 and its subdomains, S1
A
and S1
B
. Cell binding and invasion inhibition assays revealed a preference for the S1
B
subdomain in binding to the receptors on the cell surface, and this unknown receptor is not utilized by the porcine epidemic diarrhea virus. Nanoparticle display demonstrated hemagglutination of erythrocytes from pigs, humans, and mice, linking the S1
A
subdomain to the binding of sialic acid (Sia) involved in virus attachment. We successfully rescued GFP-labeled SADS-CoV (rSADS-GFP) from a recombinant cDNA clone to track viral infection. Antisera raised against S1, S1
A
, or S1
B
contained highly potent neutralizing antibodies, with anti-S1
B
showing better efficiency in neutralizing rSADS-GFP infection compared to anti-S1
A
. Furthermore, depletion of heparan sulfate (HS) by heparinase treatment or pre-incubation of rSADS-GFP with HS or constituent monosaccharides could inhibit SADS-CoV entry. Finally, we demonstrated that active furin cleavage of S glycoprotein and the presence of type II transmembrane serine protease (TMPRSS2) are essential for SADS-CoV infection. These combined observations suggest that the wide cell tropism of SADS-CoV may be related to the distribution of Sia or HS on the cell surface, whereas the S1
B
contains the main protein receptor binding site. Specific host proteases also play important roles in facilitating SADS-CoV entry.

IMPORTANCE

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel pathogen infecting piglet, and its unique genetic evolution characteristics and broad species tropism suggest the potential for cross-species transmission. The virus enters cells through its spike (S) glycoprotein. In this study, we identify the receptor binding domain on the C-terminal part of the S1 subunit (S1
B
) of SADS-CoV, whereas the sugar-binding domain located at the S1 N-terminal part of S1 (S1
A
). Sialic acid, heparan sulfate, and specific host proteases play essential roles in viral attachment and entry. The dissection of SADS-CoV S1 subunit’s functional domains and identification of cellular entry cofactors will help to explore the receptors used by SADS-CoV, which may contribute to exploring the mechanisms behind cross-species transmission and host tropism.