bluuebunny/crossref_metadata_2025 huggingface dataset:
An AI-driven clinical care pathway to reduce 30-day readmission for chronic obstructive pulmonary disease (COPD) patients
Authors: Wang Lin , Li Guihua , Ezeana Chika F. , Ogunti Richard , Puppala Mamta , He Tiancheng , Yu Xiaohui , Wong Solomon S. Y. , Yin Zheng , Roberts Aaron W. , Nezamabadi Aryan , Xu Pingyi , Frost Adaani , Jackson Robert E. , Wong Stephen T. C.
Published: December 2022

Healthcare regulatory agencies have mandated a reduction in 30-day hospital readmission rates and have targeted COPD as a major contributor to 30-day readmissions. We aimed to develop and validate a simple tool deploying an artificial neural network (ANN) for early identification of COPD patients with high readmission risk. Using COPD patient data from eight hospitals within a large urban hospital system, four variables were identified, weighted and validated. These included the number of in-patient admissions in the previous 6 months, the number of medications administered on the first day, insurance status, and the Rothman Index on hospital day one. An ANN model was trained to provide a predictive algorithm and validated on an additional dataset from a separate time period. The model was implemented in a smartphone app (Re-Admit) incorporating four input risk factors, and a clinical care plan focused on high-risk readmission candidates was then implemented. Subsequent readmission data was analyzed to assess impact. The areas under the curve of receiver operating characteristics predicting readmission with ANN is 0.77, with sensitivity 0.75 and specificity 0.67 on the separate validation data. Readmission rates in the COPD high-risk subgroup after app and clinical intervention implementation saw a significant 48% decline. Our studies show the efficacy of ANN model on predicting readmission risks for COPD patients. The AI enabled Re-Admit smartphone app predicts readmission risk on day one of the patient’s admission, allowing for early implementation of medical, hospital, and community resources to optimize and improve clinical care pathways.
Revealing the global longline fleet with satellite radar
Authors: Kroodsma David A. , Hochberg Timothy , Davis Pete B. , Paolo Fernando S. , Joo Rocío , Wong Brian A.
Published: December 2022

Because many vessels use the Automatic Identification System (AIS) to broadcast GPS positions, recent advances in satellite technology have enabled us to map global fishing activity. Understanding of human activity at sea, however, is limited because an unknown number of vessels do not broadcast AIS. Those vessels can be detected by satellite-based Synthetic Aperture Radar (SAR) imagery, but this technology has not yet been deployed at scale to estimate the size of fleets in the open ocean. Here we combine SAR and AIS for large-scale open ocean monitoring, developing methods to match vessels with AIS to vessels detected with SAR and estimate the number of non-broadcasting vessels. We reveal that, between September 2019 and January 2020, non-broadcasting vessels accounted for about 35% of the longline activity north of Madagascar and 10% of activity near French Polynesia and Kiribati’s Line Islands. We further demonstrate that this method could monitor half of the global longline activity with about 70 SAR images per week, allowing us to track human activity across the oceans.
Analysis toxicity by different methods and anxiolytic effect of the aqueous extract Lippia sidoides Cham.
Authors: Camilo Cicera J. , Leite Débora O. D. , da S. Mendes Johnatan W. , Dantas Alexandro R. , de Carvalho Natália K. G. , Castro José W. G. , Salazar Gerson J. T. , Ferreira Maria Kueirislene Amâncio , de Meneses Jane Eire Alencar , da Silva Antonio Wlisses , dos Santos Helcio S. , Tavares Josean F. , Silva Joanda P. R. e , Rodrigues Fabiola F. G. , Cheon Chunhoo , Kim Bonglee , da Costa José Galberto Martins
Published: December 2022

Lippia sidoides Cham. (Verbenaceae) is a species often mentioned in traditional medicine due to the medicinal properties attributed to its leaves, which include antibacterial, antifungal, acaricidal and antioxidant. Several of these actions have been scientifically proven, according to reports in the literature; however, little is known about toxicological aspects of this plant. This work included studies to determine the chemical composition and toxicity tests, using several methods aiming to evaluate the safety for use of the aqueous extract of L. sidoides leaves, in addition, the anxiolytic effect on adult zebrafish was investigated, thus contributing to the pharmacological knowledge and traditional medicine concerning the specie under study. The chemical profile was determined by liquid chromatography coupled to mass spectrometry-HPLC/MS with electrospray ionization. Toxicity was evaluated by zebrafish, Drosophila melanogaster, blood cells, and Artemia salina models. 12 compounds belonging to the flavonoid class were identified. In the toxicity assays, the observed results showed low toxicity of the aqueous extract in all tests performed. In the analysis with zebrafish, the highest doses of the extract were anxiolytic, neuromodulating the GABAa receptor. The obtained results support the safe use of the aqueous extract of L. sidoides leaves for the development of new drugs and for the use by populations in traditional medicine.
Spatial attention in perceptual decision making as revealed by response-locked classification image analysis
Authors: Sano Hironobu , Ueno Natsuki , Maruyama Hironori , Motoyoshi Isamu
Published: December 2022

In many situations, humans serially sample information from many locations in an image to make an appropriate decision about a visual target. Spatial attention and eye movements play a crucial role in this serial vision process. To investigate the effect of spatial attention in such dynamic decision making, we applied a classification image (CI) analysis locked to the observer’s reaction time (RT). We asked human observers to detect as rapidly as possible a target whose contrast gradually increased on the left or right side of dynamic noise, with the presentation of a spatial cue. The analysis revealed a spatiotemporally biphasic profile of the CI which peaked at ~ 350 ms before the observer’s response. We found that a valid cue presented at the target location shortened the RT and increased the overall amplitude of the CI, especially when the cue appeared 500–1250 ms before the observer's response. The results were quantitatively accounted for by a simple perceptual decision mechanism that accumulates the outputs of the spatiotemporal contrast detector, whose gain is increased by sustained attention to the cued location.
Helical stability of the GnTV transmembrane domain impacts on SPPL3 dependent cleavage
Authors: Papadopoulou Alkmini A. , Stelzer Walter , Silber Mara , Schlosser Christine , Spitz Charlotte , Haug-Kröper Martina , Straub Tobias , Müller Stephan A. , Lichtenthaler Stefan F. , Muhle-Goll Claudia , Langosch Dieter , Fluhrer Regina
Published: December 2022

Signal-Peptide Peptidase Like-3 (SPPL3) is an intramembrane cleaving aspartyl protease that causes secretion of extracellular domains from type-II transmembrane proteins. Numerous Golgi-localized glycosidases and glucosyltransferases have been identified as physiological SPPL3 substrates. By SPPL3 dependent processing, glycan-transferring enzymes are deactivated inside the cell, as their active site-containing domain is cleaved and secreted. Thus, SPPL3 impacts on glycan patterns of many cellular and secreted proteins and can regulate protein glycosylation. However, the characteristics that make a substrate a favourable candidate for SPPL3-dependent cleavage remain unknown. To gain insights into substrate requirements, we investigated the function of a GxxxG motif located in the transmembrane domain of N-acetylglucosaminyltransferase V (GnTV), a well-known SPPL3 substrate. SPPL3-dependent secretion of the substrate’s ectodomain was affected by mutations disrupting the GxxxG motif. Using deuterium/hydrogen exchange and NMR spectroscopy, we studied the effect of these mutations on the helix flexibility of the GnTV transmembrane domain and observed that increased flexibility facilitates SPPL3-dependent shedding and vice versa. This study provides first insights into the characteristics of SPPL3 substrates, combining molecular biology, biochemistry, and biophysical techniques and its results will provide the basis for better understanding the characteristics of SPPL3 substrates with implications for the substrates of other intramembrane proteases.
Sustainability analysis of bioethanol production from grain and tuber starchy feedstocks
Authors: Sanni A. , Olawale A. S. , Sani Y. M. , Kheawhom S.
Published: December 2022

A comparative sustainability study of bioethanol production from selected starchy feedstocks that are abundantly available was carried out in this work. This is to ensure the safe, reliable, and efficient production and consumption of fuel-grade bioethanol. The analysis utilised the established economic minimum bioethanol plant capacity of 158,000 m3/annum. The processing flowsheet model utilised was the same for each feedstock. The sustainability study's economic, environmental, and energy perspectives were investigated. The economic and environmental indices were assessed using Superpro Designer and openLCA sustainability software, respectively. Exergy and lost work were subsequently estimated manually with Microsoft Excel. The economic analyses showed that the plants using cassava and sweet potato initially had the highest return on investment (ROI) of 64.41 and 41.96% respectively at a minimum of 80% plants’ capacity utilisation. The break-even point occurs at a bioethanol price of $3.27 per gallon, beyond which positive net present values were obtained for the four processes. The least profitable plant was based on sorghum recording an ROI value of 34.11%. The environmental assessment on the four selected feedstocks showed that the processes based on cassava, corn, sweet potato, and sorghum recorded encouraging global warming potential (GWP) of 0.2452, 0.2067, 2.5261, and 0.2099 kg CO2 equivalent respectively. Cassava and corn emerged as the two most economically viable feedstocks when economic parameters were adjusted to include pollutants emission/discharge costs but with a slight decrease in profitability indices. The lost work analyses showed that distillation columns were the least energy-efficient units in the four bioethanol production routes assessed, recording loss work of about 61, 68, 34, and 49 MW for cassava, sweet potato, corn, and sorghum processing plants respectively. However, the net energy balance (NEB) and energy renewability results of the four production routes showed that the processes utilising the four selected starchy biomass feedstocks are more sustainable compared to fossil fuels.
TDAG51 deficiency attenuates dextran sulfate sodium-induced colitis in mice
Authors: Jeon Hyoeun , Amarasekara Dulshara Sachini , Lee Nari , Park Hye-Won , Yu Jiyeon , Rho Jaerang
Published: December 2022

Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn’s disease, is a group of chronic inflammatory diseases of the gastrointestinal tract. Although the multifactorial etiology of IBD pathogenesis is relatively well documented, the regulatory factors that confer a risk of IBD pathogenesis remain less explored. In this study, we report that T-cell death-associated gene 51 (TDAG51/PHLDA1) is a novel regulator of the development of dextran sulfate sodium (DSS)-induced colitis in mice. TDAG51 expression was elevated in the colon tissues of DSS-induced experimental colitis mice. TDAG51 deficiency protected mice against acute DSS-induced lethality and body weight changes and disease severity. DSS-induced structural damage and mucus secretion in colon tissues were significantly reduced in TDAG51-deficient mice compared with wild-type mice. We observed similar results in a DSS-induced chronic colitis mouse model. Finally, we showed that the production of inflammatory mediators, including proinflammatory enzymes, molecules and cytokines, was decreased in DSS-treated TDAG51-deficient mice compared with DSS-treated wild-type mice. Thus, we demonstrated that TDAG51 deficiency plays a protective role against DSS-induced colitis by decreasing the production of inflammatory mediators in mice. These findings suggest that TDAG51 is a novel regulator of the development of DSS-induced colitis and is a potential therapeutic target for IBD.
Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
Authors: Rodrigo María Jesús , Bravo-Osuna Irene , Subias Manuel , Montolío Alberto , Cegoñino José , Martinez-Rincón Teresa , Mendez-Martinez Silvia , Aragón-Navas Alba , Garcia-Herranz David , Pablo Luis Emilio , Herrero-Vanrell Rocío , del Palomar Amaya Pérez , Garcia-Martin Elena
Published: December 2022

This study compares four different animal models of chronic glaucoma against normal aging over 6 months. Chronic glaucoma was induced in 138 Long–Evans rats and compared against 43 aged-matched healthy rats. Twenty-five rats received episcleral vein sclerosis injections (EPIm cohort) while the rest were injected in the eye anterior chamber with a suspension of biodegradable microspheres: 25 rats received non-loaded microspheres (N-L Ms cohort), 45 rats received microspheres loaded with dexamethasone (MsDexa cohort), and 43 rats received microspheres co-loaded with dexamethasone and fibronectin (MsDexaFibro cohort). Intraocular pressure, neuroretinal function, structure and vitreous interface were evaluated. Each model caused different trends in intraocular pressure, produced specific retinal damage and vitreous signals. The steepest and strongest increase in intraocular pressure was seen in the EPIm cohort and microspheres models were more progressive. The EPIm cohort presented the highest vitreous intensity and percentage loss in the ganglion cell layer, the MsDexa cohort presented the greatest loss in the retinal nerve fiber layer, and the MsDexaFibro cohort presented the greatest loss in total retinal thickness. Function decreased differently among cohorts. Using biodegradable microspheres models it is possible to generate tuned neurodegeneration. These results support the multifactorial nature of glaucoma based on several noxa.
Cryptic taxonomic diversity and high-latitude melanism in the glossiphoniid leech assemblage from the Eurasian Arctic
Authors: Bolotov Ivan N. , Kondakov Alexander V. , Eliseeva Tatyana A. , Aksenova Olga V. , Babushkin Evgeny S. , Bespalaya Yulia V. , Chertoprud Elena S. , Dvoryankin Gennady A. , Gofarov Mikhail Yu. , Klass Anna L. , Konopleva Ekaterina S. , Kropotin Alexander V. , Lyubas Artem A. , Makhrov Alexander A. , Palatov Dmitry M. , Shevchenko Alexander R. , Sokolova Svetlana E. , Spitsyn Vitaly M. , Tomilova Alena A. , Vikhrev Ilya V. , Zubrii Natalia A. , Vinarski Maxim V.
Published: December 2022

The family Glossiphoniidae is a diverse and widespread clade of freshwater leeches, playing a significant role in functioning of aquatic ecosystems. The taxonomy and biogeography of leeches from temperate, subtropical, and tropical regions attracted much attention of zoologists, while their taxonomic richness and distribution in the Arctic are poorly understood. Here, we present an overview of the Eurasian Arctic Glossiphoniidae based on the most comprehensive occurrence and DNA sequence datasets sampled to date. This fauna contains 14 species, belonging to five genera and three subfamilies. One genus and five species are new to science and described here. The world’s northernmost occurrences of glossiphoniids are situated on the Taymyr Peninsula at 72° N, although further records at higher latitudes are expected. Most Arctic leeches are characterized by broad ranges crossing several climatic zones (e.g., Glossiphonia balcanica and G. nebulosa), although the distribution of two new species may be confined to the high-latitude areas. The Taymyr Peninsula with the nearby Putorana Plateau represents the most species-rich area (totally 9 species), while the European Arctic, Iceland, Kolyma Highland, and Chukotka Peninsula house depleted faunas (2–4 species per subregion). Finally, we show that the high-latitude melanism is a common phenomenon in glossiphoniid leeches.
Layer separation mapping and consolidation evaluation of a fifteenth century panel painting using terahertz time-domain imaging
Authors: Lambert Frances E. M. , Ornik Jan , Staats Naja-Anissa , Jäckel Alexander , Hernandez-Cardoso Goretti G. , Taiber Jochen , Stübling Eva-Maria , Rudolph Benjamin , Mack Oliver , Portsteffen Hans , Castro-Camus Enrique , Koch Martin
Published: December 2022

Over time, artworks often sustain paint layer separation and air gaps within their internal structure due to storage conditions and past restoration efforts. Because of this, paint layer consolidation interventions are an essential activity for art conservators. However, it is difficult to determine the exact location and the extent of layer separation on a piece of art in a non-invasive way, and even more difficult to evaluate the success of a consolidation intervention. In this work, a fifteenth-century wood panel painting was analyzed using terahertz time-domain imaging before and after it was consolidated. Using the terahertz data, it was possible to determine the areas on the artwork in need of consolidation and aid the intervention. The analysis of the after data allowed for the control and determination of the success of the consolidation effort in a non-destructive manner.
Evaluation of the biocompability and corrosion activity of resorbable CaMgZnYbBAu alloys
Authors: Szyba Dawid , Kubina Robert , Młynarek-Żak Katarzyna , Radoń Adrian , Kania Aneta , Babilas Rafał
Published: December 2022

Calcium-based alloys can be promising candidates for use as biodegradable implants because of attractive properties as mechanical, corrosive, and biocompatible. In the work, the biocompatibility authors discussed the results of the Ca32Mg12Zn38Yb18−xBx(x = 0, 1, 2, 3 at.%) and Ca32Mg12Zn38Yb18−2xBxAux(x = 1, 2 at.%) alloys. The tests were performed using a MTT assay. The corrosion behavior of such Ca-based alloys in PWE fluid at 37 °C was studied and compared with the results in Ringer’s solution from previous works. Electrochemical tests were presented by open circuit potential and potentiodynamic curves. Different concentrations of boron and gold in the alloys caused changes in the corrosion results. The best corrosion resistance in PWE solution was observed for the Ca-based alloy with 2 at.% Au due to the lowest value of the corrosion current density (jcorr), equal to 10.6 µA·cm−2. A slightly higher value ofjcorrwas obtained for the Ca32Mg12Zn38Yb15B3alloy with the lowest roughness values. The results of the cytotoxicity tests also showed that the alloy with 3 at.% boron was characterized by the highest cell viability. The investigation results discussed in the work allow us to suggest that the presented calcium alloys with 3 at.% of B, and 2 at.% of Au addition may be promising materials for the use in implantology.
Characterizing trends and associations for hepatitis C virus antibody prevalence in the Middle East and North Africa: meta-regression analyses
Authors: Mahmud Sarwat , Chemaitelly Hiam , Alaama Ahmed S. , Hermez Joumana G. , Abu-Raddad Laith J.
Published: December 2022

This study characterized population-level trends and associations with hepatitis C virus (HCV) antibody (Ab) prevalence in the Middle East and North Africa (MENA). Data source was the standardized and systematically gathered MENA HCV Epidemiology Synthesis Project Database. Random-effects univariable and multivariable meta-regressions were conducted. 2,621 HCV Ab prevalence measures on 49,824,108 individuals were analyzed. In the analysis including all populations, 71% of the variation in prevalence was explained, mostly by at-risk population type. Compared to the general population, prevalence was 23-fold higher among people who inject drugs, and 14-fold higher among high-risk clinical populations. In the analysis including only the general population, 67% of the variation in prevalence was explained, mostly by country/subregion. Compared to Afghanistan, prevalence was highest in Egypt and Pakistan. Prevalence in the general population was declining at a rate of 4% per year, but outside the general population, the decline was at only 1% per year. HCV Ab prevalence in MENA is declining rapidly, but this decline is largely occurring in the general population following introduction of blood and injection safety measures. The decline in populations at higher risk of exposure is slow and below the level needed to achieve HCV elimination by 2030.
Association between serum lipid levels over time and risk of Parkinson’s disease
Authors: Hurh Kyungduk , Park Minah , Jang Sung-in , Park Eun-Cheol , Jang Suk-Yong
Published: December 2022

The role of serum lipids in Parkinson’s disease (PD) remains controversial. We aimed to evaluate the association between time-varying serum lipid levels and the risk of PD. This study included an assessment of the complete lipid profiles of 200,454 individuals from the 2002–2019 Korean National Health Insurance Health Screening Cohort. Time-dependent Cox proportional hazard regression models were used to evaluate the association between serum lipid levels over time and the risk of PD. Individuals in the lowest tertile of total cholesterol and low-density lipoprotein cholesterol had a 1.17 times [hazard ratio (HR) 1.17; 95% confidence interval (CI) 1.04–1.31] and 1.19 times (HR 1.19; 95% CI 1.06–1.34) higher risk of PD than those in middle tertile, respectively. Individuals in the highest high-density lipoprotein cholesterol tertile had a 0.89 times (HR 0.89; 95% CI 0.79–1.00) lower risk of PD than those in middle tertile, but the association was less robust in sensitivity analyses. Serum triglyceride levels were not related to the risk of PD. Our results suggest that the serum total and low-density lipoprotein cholesterol levels over time are inversely associated with the risk of PD. Further research is warranted to confirm these findings and reveal the underlying mechanisms.
Comparison of RT-dPCR and RT-qPCR and the effects of freeze–thaw cycle and glycine release buffer for wastewater SARS-CoV-2 analysis
Authors: Huge Bonnie Jaskowski , North Devin , Mousseau C. Bruce , Bibby Kyle , Dovichi Norman J. , Champion Matthew M.
Published: December 2022

Public health efforts to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic rely on accurate information on the spread of the disease in the community. Acute and surveillance testing has been primarily used to characterize the extent of the disease. However, obtaining a representative sample of the human population is challenging because of limited testing capacity and incomplete testing compliance. Wastewater-based epidemiology is an agnostic alternative to surveillance testing that provides an average sample from the population served by the treatment facility. We compare the performance of reverse transcription quantitative PCR (RT-qPCR) and reverse transcription digital droplet PCR (RT-dPCR) for analysis of SARS-CoV-2 RNA in a regional wastewater treatment facility in northern Indiana, USA from the earliest stages of the pandemic. 1-L grab samples of wastewater were clarified and concentrated. Nucleic acids were extracted from aliquots and analyzed in parallel using the two methods. Synthetic viral nucleic acids were used for method development and generation of add-in standard-curves. Both methods were highly sensitive in detecting SARS-CoV-2 in wastewater, with detection limits as low as 1 copy per 500 mL wastewater. RT-qPCR and RT-dPCR provided essentially identical coefficients of variation (s/$$\overline{\mathrm{x} }$$

x
¯

 = 0.15) for triplicate measurements made on wastewater samples taken on 16 days. We also observed a sevenfold decrease in viral load from a grab sample that was frozen at – 80 °C for 92 days compared to results obtained without freezing. Freezing samples before analysis should be discouraged. Finally, we found that treatment with a glycine release buffer resulted in a fourfold inhibition in RT-qPCR signal; treatment with a glycine release buffer also should be discouraged. Despite their prevalence and convenience in wastewater analysis, glycine release and freezing samples severely and additively (~ tenfold) degraded recovery and detection of SARS-CoV-2.
The trend and spatial spread of multisectoral climate extremes in CMIP6 models
Authors: Adeyeri Oluwafemi E. , Zhou Wen , Wang Xuan , Zhang Ruhua , Laux Patrick , Ishola Kazeem A. , Usman Muhammad
Published: December 2022

Climate change could exacerbate extreme climate events. This study investigated the global and continental representations of fourteen multisectoral climate indices during the historical (1979–2014), near future (2025–2060) and far future (2065–2100) periods under two emission scenarios, in eleven Coupled Model Intercomparison Project (CMIP) General Circulation Models (GCM). We ranked the GCMs based on five metrics centred on their temporal and spatial performances. Most models followed the reference pattern during the historical period. MPI-ESM ranked best in replicating the daily precipitation intensity (DPI) in Africa, while CANESM5 GCM ranked first in heatwave index (HI), maximum consecutive dry days (MCCD). Across the different continents, MPI-LR GCM performed best in replicating the DPI, except in Africa. The model ranks could provide valuable information when selecting appropriate GCM ensembles when focusing on climate extremes. A global evaluation of the multi-index causal effects for the various indices shows that the dry spell total length (DSTL) was the most crucial index modulating the MCCD for all continents. Also, most indices exhibited a positive climate change signal from the historical to the future. Therefore, it is crucial to design appropriate strategies to strengthen resilience to extreme climatic events while mitigating greenhouse gas emissions.
The evolutionary extortion game of multiple groups in hypernetworks
Authors: Shen Aizhong , Gao Zilin , Gao Xiang , Cui Dan
Published: December 2022

As a type of zero-determinant strategies, the extortion strategy was found to be an evolutionarily stable strategy in structural groups. However, instead of complex networks structure, this paper focus on a multi-group game in hypernetworks, using the framework of a gift giving game driven by replicator-like dynamics. We find that the extortion is evolutionarily stable in the hypernetwork structure. The extortion game in hypernetworks can promote the emergence of the cooperative behavior compared to the traditional dual-strategy game and the extortion game in complex networks. The results show that the cooperation behavior attracts most of the groups for the smaller benefit value. With the increase of benefit value, cooperators turn into defectors and extortioners, but cooperation behavior still survives in hypernetworks under extreme conditions. Moreover, small-scale groups are more conducive to cooperation.
Sex differences in the inflammatory response of the mouse DRG and its connection to pain in experimental autoimmune encephalomyelitis
Authors: Maguire Aislinn D. , Friedman Timothy N. , Villarreal Andrade Dania N. , Haq Fajr , Dunn Jacob , Pfeifle Keiana , Tenorio Gustavo , Buro Karen , Plemel Jason R. , Kerr Bradley J.
Published: December 2022

Multiple Sclerosis (MS) is an autoimmune disease with notable sex differences. Women are not only more likely to develop MS but are also more likely than men to experience neuropathic pain in the disease. It has been postulated that neuropathic pain in MS can originate in the peripheral nervous system at the level of the dorsal root ganglia (DRG), which houses primary pain sensing neurons (nociceptors). These nociceptors become hyperexcitable in response to inflammation, leading to peripheral sensitization and eventually central sensitization, which maintains pain long-term. The mouse model experimental autoimmune encephalomyelitis (EAE) is a good model for human MS as it replicates classic MS symptoms including pain. Using EAE mice as well as naïve primary mouse DRG neurons cultured in vitro, we sought to characterize sex differences, specifically in peripheral sensory neurons. We found sex differences in the inflammatory profile of the EAE DRG, and in the TNFα downstream signaling pathways activated intracellularly in cultured nociceptors. We also found increased cell death with TNFα treatment. Given that TNFα signaling has been shown to initiate intrinsic apoptosis through mitochondrial disruption, this led us to investigate sex differences in the mitochondria’s response to TNFα. Our results demonstrate that male sensory neurons are more sensitive to mitochondrial stress, making them prone to neuronal injury. In contrast, female sensory neurons appear to be more resistant to mitochondrial stress and exhibit an inflammatory and regenerative phenotype that may underlie greater nociceptor hyperexcitability and pain. Understanding these sex differences at the level of the primary sensory neuron is an important first step in our eventual goal of developing sex-specific treatments to halt pain development in the periphery before central sensitization is established.
The challenge of community mental health interventions with patients, relatives, and health professionals during the COVID-19 pandemic: a real-world 9-month follow-up study
Authors: Roncero Carlos , González-Sánchez Armando , Pérez-Laureano Ángela , Ortiz-Fune Carmen , Díaz-Trejo Sara , Bersabé-Pérez Miriam , Braquehais María Dolores , Pérez-Rodríguez Javier , Maderuelo-Fernández José Ángel , Benito-Sánchez José Antonio
Published: December 2022

Since the beginning of the COVID-19 pandemic, the need to implement protocols that respond to the mental health demands of the population has been demonstrated. The PASMICOR programme started in March 2020, involving a total of 210 requests for treatment. Out of those subjects, the intervention was performed in 53 patients with COVID-19 without history of past psychiatric illness, 57 relatives and 60 health professionals, all of them within the area of Salamanca (Spain). Interventions were carried out by professionals of the public mental health service mostly by telephone. Depending on clinical severity, patients received basic (level I) or complex psychotherapeutic care combined with psychiatric care (level II). The majority of attended subjects were women (76.5%). Anxious-depressive symptoms were predominant, although sadness was more frequent in patients, insomnia in relatives and anxiety and fear in health professionals. 80% of the sample, particularly most of the health professionals, required a high-intensity intervention (level II). Nearly 50% of the people treated were discharged after an average of 5 interventions. Providing early care to COVID-19 patients, relatives and professionals by using community mental health resources can help to reduce the negative impact of crises, such as the pandemic, on the most affected population groups.
Non-targeted detection of food adulteration using an ensemble machine-learning model
Authors: Chung Teresa , Tam Issan Yee San , Lam Nelly Yan Yan , Yang Yanni , Liu Boyang , He Billy , Li Wengen , Xu Jie , Yang Zhigang , Zhang Lei , Cao Jian Nong , Lau Lok-Ting
Published: December 2022

Recurrent incidents of economically motivated adulteration have long-lasting and devastating effects on public health, economy, and society. With the current food authentication methods being target-oriented, the lack of an effective methodology to detect unencountered adulterants can lead to the next melamine-like outbreak. In this study, an ensemble machine-learning model that can help detect unprecedented adulteration without looking for specific substances, that is, in a non-targeted approach, is proposed. Using raw milk as an example, the proposed model achieved an accuracy and F1 score of 0.9924 and 0. 0.9913, respectively, when the same type of adulterants was presented in the training data. Cross-validation with spiked contaminants not routinely tested in the food industry and blinded from the training data provided an F1 score of 0.8657. This is the first study that demonstrates the feasibility of non-targeted detection with no a priori knowledge of the presence of certain adulterants using data from standard industrial testing as input. By uncovering discriminative profiling patterns, the ensemble machine-learning model can monitor and flag suspicious samples; this technique can potentially be extended to other food commodities and thus become an important contributor to public food safety.
Differential diagnosis of common etiologies of left ventricular hypertrophy using a hybrid CNN-LSTM model
Authors: Hwang In-Chang , Choi Dongjun , Choi You-Jung , Ju Lia , Kim Myeongju , Hong Ji-Eun , Lee Hyun-Jung , Yoon Yeonyee E. , Park Jun-Bean , Lee Seung-Pyo , Kim Hyung-Kwan , Kim Yong-Jin , Cho Goo-Yeong
Published: December 2022

Differential diagnosis of left ventricular hypertrophy (LVH) is often obscure on echocardiography and requires numerous additional tests. We aimed to develop a deep learning algorithm to aid in the differentiation of common etiologies of LVH (i.e. hypertensive heart disease [HHD], hypertrophic cardiomyopathy [HCM], and light-chain cardiac amyloidosis [ALCA]) on echocardiographic images. Echocardiograms in 5 standard views (parasternal long-axis, parasternal short-axis, apical 4-chamber, apical 2-chamber, and apical 3-chamber) were obtained from 930 subjects: 112 with HHD, 191 with HCM, 81 with ALCA and 546 normal subjects. The study population was divided into training (n = 620), validation (n = 155), and test sets (n = 155). A convolutional neural network-long short-term memory (CNN-LSTM) algorithm was constructed to independently classify the 3 diagnoses on each view, and the final diagnosis was made by an aggregate network based on the simultaneously predicted probabilities of HCM, HCM, and ALCA. Diagnostic performance of the algorithm was evaluated by the area under the receiver operating characteristic curve (AUC), and accuracy was evaluated by the confusion matrix. The deep learning algorithm was trained and verified using the training and validation sets, respectively. In the test set, the average AUC across the five standard views was 0.962, 0.982 and 0.996 for HHD, HCM and CA, respectively. The overall diagnostic accuracy was significantly higher for the deep learning algorithm (92.3%) than for echocardiography specialists (80.0% and 80.6%). In the present study, we developed a deep learning algorithm for the differential diagnosis of 3 common LVH etiologies (HHD, HCM and ALCA) by applying a hybrid CNN-LSTM model and aggregate network to standard echocardiographic images. The high diagnostic performance of our deep learning algorithm suggests that the use of deep learning can improve the diagnostic process in patients with LVH.
Sustainable fashion: eco-friendly dyeing of wool fiber with novel mixtures of biodegradable natural dyes
Authors: Lin Lina , Jiang Tiancheng , Xiao Lexin , Pervez Md. Nahid , Cai Xiaobo , Naddeo Vincenzo , Cai Yingjie
Published: December 2022

Natural materials, especially natural colorants, have achieved global prominence and might be regarded as an environmentally beneficial alternative to hazardous synthetic dyes. The color limitation of natural dyes hinders their application in textiles. The present work aims to prepare more color shades of wool yarns via dyeing with ternary natural dye mixtures without adding mordants. In this study, a sustainable dyeing approach for wool yarn was evaluated with three natural dyes, madder red (MR), gardenia blue (GB), and gardenia yellow (GY), by following an industrial dyeing procedure in the absence of a mordant. In the beginning, a preliminary assessment of dye stabilities was carried out, and it was found that the three natural dyes were sensitive to temperature and acid (degradation tendency). Then, the dyeing behavior was systematically evaluated, including a single natural dye, a binary natural dye mixture, and a ternary natural dye mixture. The results of wool yarn dyeing with a single natural dye show that the dye exhaustion percentage (E%) of MR, GY, and GB was in the ranges of 78.7–94.1%, 13.4–44.1%, and 54.8–68.5%, respectively. The dyeing results of wool yarns dyed with binary and ternary natural dye mixtures (a color triangle framework of dyed wool yarn) were characterized by colorimetric values (L*, a*, b*, C*, h, and K/S), and are presented to enlighten various colorful shades. Finally, color uniformity and colorfastness tests confirmed the vital contribution of natural dyes toward wool yarn coloration. Particularly, colorfastness to washing confirmed the stability of natural dyes with reference to the lower amount of dyes released into the effluent, which is beneficial for the environment. Overall, this study provides a good background for enhancing the industrialization trend of natural dyes by modulating their dyeing scheme.
Longitudinal stability in working memory and frontal activity in relation to general brain maintenance
Authors: Nyberg Lars , Karalija Nina , Papenberg Goran , Salami Alireza , Andersson Micael , Pedersen Robin , Vikner Tomas , Garrett Douglas D. , Riklund Katrine , Wåhlin Anders , Lövdén Martin , Lindenberger Ulman , Bäckman Lars
Published: December 2022

Cognitive functions are well-preserved for some older individuals, but the underlying brain mechanisms remain disputed. Here, 5-year longitudinal 3-back in-scanner and offline data classified individuals in a healthy older sample (baseline age = 64–68 years) into having stable or declining working-memory (WM). Consistent with a vital role of the prefrontal cortex (PFC), WM stability or decline was related to maintained or reduced longitudinal PFC functional responses. Subsequent analyses of imaging markers ofgeneralbrain maintenance revealed higher levels in the stable WM group on measures of neurotransmission and vascular health. Also, categorical and continuous analyses showed that rate of WM decline was related to global (ventricles) and local (hippocampus) measures of neuronal integrity. Thus, our findings support a role of the PFC as well as general brain maintenance in explaining heterogeneity in longitudinal WM trajectories in aging.
Pirenperone relieves the symptoms of fragile X syndrome in Fmr1 knockout mice
Authors: Kim Yujeong , Jeon Se Jin , Gonzales Edson Luck , Shin Dongpil , Remonde Chilly Gay , Ahn TaeJin , Shin Chan Young
Published: December 2022

Fragile X syndrome (FXS) is a neurodevelopmental disorder that is caused by the loss of Fragile X-linked mental retardation protein (FMRP), an RNA binding protein that can bind and recognize different RNA structures and regulate the target mRNAs’ translation involved in neuronal synaptic plasticity. Perturbations of this gene expression network have been related to abnormal behavioral symptoms such as hyperactivity, and impulsivity. Considering the roles of FMRP in the modulation of mRNA translation, we investigated the differentially expressed genes which might be targeted to revert to normal and ameliorate behavioral symptoms. Gene expression data was analyzed and used the connectivity map (CMap) to understand the changes in gene expression in FXS and predict the effective drug candidates. We analyzed the GSE7329 dataset that had 15 control and 8 FXS patients’ lymphoblastoid samples. Among 924 genes, 42 genes were selected as signatures for CMap analysis, and 24 associated drugs were found. Pirenperone was selected as a potential drug candidate for FXS for its possible antipsychotic effect. Treatment of pirenperone increased the expression level of Fmr1 gene. Moreover, pirenperone rescued the behavioral deficits in Fmr1 KO mice including hyperactivity, spatial memory, and impulsivity. These results suggest that pirenperone is a new drug candidate for FXS, which should be verified in future studies.
Spin and charge drift-diffusion in ultra-scaled MRAM cells
Authors: Fiorentini Simone , Bendra Mario , Ender Johannes , de Orio Roberto L. , Goes Wolfgang , Selberherr Siegfried , Sverdlov Viktor
Published: December 2022

Designing advanced single-digit shape-anisotropy MRAM cells requires an accurate evaluation of spin currents and torques in magnetic tunnel junctions (MTJs) with elongated free and reference layers. For this purpose, we extended the analysis approach successfully used in nanoscale metallic spin valves to MTJs by introducing proper boundary conditions for the spin currents at the tunnel barrier interfaces, and by employing a conductivity locally dependent on the angle between the magnetization vectors for the charge current. The experimentally measured voltage and angle dependencies of the torques acting on the free layer are thereby accurately reproduced. The switching behavior of ultra-scaled MRAM cells is in agreement with recent experiments on shape-anisotropy MTJs. Using our extended approach is absolutely essential to accurately capture the interplay of the Slonczewski and Zhang-Li torque contributions acting on a textured magnetization in composite free layers with the inclusion of several MgO barriers.
A survey of allergic conjunctivitis in children in China
Authors: Gao Xu , Hong Lan , Xiang Qin
Published: December 2022

To explore the risk factors for allergic conjunctivitis (AC) in Chinese children. We recruited 176 children who suffered from AC and a control group comprising 131 normal subjects in southern China. Each participant completed a questionnaire and underwent multiple eye examinations and a skin prick test (SPT). The data of the questionnaire, the scores of the symptoms/signs and the results of the SPT were analysed. The rate of parental allergic history in the case group was much higher than that in the control group (P < 0.01). Compared with the control group, the case group was more likely to have other concomitant diseases (P < 0.01). The scores of ocular symptoms/signs had a significant correlation with the clinical duration of AC in the case group (P < 0.01). Children with other concomitant diseases or a parental allergic history were more likely to have AC.
Electronic cigarettes use and ‘dual use’ among the youth in 75 countries: estimates from Global Youth Tobacco Surveys (2014–2019)
Authors: Sreeramareddy Chandrashekhar T. , Acharya Kiran , Manoharan Anusha
Published: December 2022

We report the country-level prevalence of awareness about electronic cigarette use, and ‘dual use’ and its association with age, sex, country income, and e-cigarette regulatory status. We analyzed the most recent Global Youth Tobacco Surveys done on nationally representative samples of school-going youth aged 13–15 years in 75 countries/territories. The weighted prevalence of ‘awareness’ (heard about e-cigarettes), ‘ever use’ (even tried a few puffs), ‘current use’ (during the last 30 days), and ‘dual use’ (e-cigarette use and cigarette smoking during the last 30 days) were estimated. Awareness was > 80% in 13 countries mostly from Europe Poland being the highest at 95.8% (95% CI 94.8- 96.6). In seven countries, 30–50% of the youth had ever used an e-cigarette, Italy was the highest at 55.1% (95%CI 51–3,58.9). In 30 countries, current e-cigarette use was > 10%, the highest of 35.1% (95%CI 32.4–38.0) in Guam. Awareness and use were highest in the European region (74.6% and 34.5%) and HIC (83.6% and 39.4%). Youth from HIC (compared to lMIC) and countries having restrictive e-cigarette regulations (compared to NRP) had 2.4 times (aOR 2.2.4, 95% CI 2.2, 2.7) and 1.8 times (aOR 1.8, 95% CI 1.6, 2.0) higher odds of being current e-cigarette users respectively. Youth in countries with the most restrictive e-cigarette regulations (compared to NRP) had 0.6 times lower odds of being current e-cigarette users (aOR 0.6, 95% CI 0.6, 0.7). Awareness and e-cigarette use varied by sex, country income level, and region. Continued global surveillance of youth e-cigarette use is needed for the formulation of e-cigarette regulatory policy. Awareness and use of e-cigarettes were higher among boys, in countries in Europe and America regions, and among those with higher income and restrictive policies, whereas it was lower in countries having the most restrictive policies. Higher awareness is strongly correlated with a trial and current use of e-cigarettes. E-cigarette marketing should be restricted, and continued surveillance of e-cigarette use is needed. Most restrictive policies such as the ban on e-cigarettes appear to reduce e-cigarette use among the youth.
Cloncurry buffel grass mitigated Cr(III) and Cr(VI) toxicity in tomato plant
Authors: Shoaib Amna , Khurshid Saba , Javaid Arshad
Published: December 2022

Contamination of agricultural soil with chromium (Cr) ions has threatened global crop, human and ecosystem health. Its two oxidation states viz. Cr(III) and Cr(VI) are most stable and readily available to the plants. The study explored the impact of increasing exposure (up to 500 ppm) of Cr(III) and Cr(VI) on bio-physical traits of 15-day-old seedlings (in vitro) as well as 60-day-old tomato plant (in vivo), and highlighted the importance of buffel grass (Cenchrus pennisetiformis) in mitigating Cr levels in the tomato plants. In vitro, Petri plate bioassays with 13 different concentrations (20–500 ppm) of Cr(III) and Cr(VI) depicted the highly toxic effect of metal ions ≥ 200 ppm on all bio-physical traits of tomato seedlings. In vivo, soil spiked with Cr(III) and Cr(VI) (200, 300, and 400 mg/kg) was amended with 1% and 2% dry biomass of buffel grass. Phytotoxicity was higher in Cr(VI)-spiked soil compared with Cr(III)-spiked soil. Cr was mainly accumulated in tomato roots, and more Cr was translocated from roots to shoots from Cr(VI)-spiked soil than Cr(III)-spiked soil. Soil amendments with 2% weed biomass reduced metal toxicity in plants, particularly at 200 and 300 mg/kg of Cr. Protein profiles through SDS-PAGE revealed 12–50 kDa (mainly PR proteins) as an important region in tomato leaf, where many new bands were expressed under different treatments, particularly in the treatments provided with buffel grass. PCA-based biplot clearly separated Cr tolerance treatments from highly sensitive treatments. For the cultivation of tomato plants in Cr(III) and Cr(VI) contaminated soil (200 and 300 mg/kg), the biomass of Cloncurry buffel grass should be considered an effective and easily available phyto-management option.
Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis
Authors: Murai Shin , Takakura Kanako , Sumiyama Kenta , Moriwaki Kenta , Terai Kenta , Komazawa-Sakon Sachiko , Seki Takao , Yamaguchi Yoshifumi , Mikami Tetuo , Araki Kimi , Ohmuraya Masaki , Matsuda Michiyuki , Nakano Hiroyasu
Published: December 2022

Necroptosis is a regulated form of cell death involved in various pathological conditions, including ischemic reperfusion injuries, virus infections, and drug-induced tissue injuries. However, it is not fully understood when and where necroptosis occurs in vivo. We previously generated a Forster resonance energy transfer (FRET) biosensor, termed SMART (the sensor for MLKL activation by RIPK3 based on FRET), which monitors conformational changes of MLKL along with progression of necroptosis in human and murine cell lines in vitro. Here, we generate transgenic (Tg) mice that express the SMART biosensor in various tissues. The FRET ratio is increased in necroptosis, but not apoptosis or pyroptosis, in primary cells. Moreover, the FRET signals are elevated in renal tubular cells of cisplatin-treated SMART Tg mice compared to untreated SMART Tg mice. Together, SMART Tg mice may provide a valuable tool for monitoring necroptosis in different types of cells in vitro and in vivo.