bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting Scale Thickness in Oil Pipelines Using Frequency Characteristics and an Artificial Neural Network in a Stratified Flow Regime
Authors: Chen Tzu-Chia , Iliyasu Abdullah M. , Hanus Robert , Salama Ahmed S. , Hirota Kaoru
Published: October 2022

One of the main problems in oil fields is the deposition of scale inside oil pipelines, which causes problems such as the reduction of the internal diameter of oil pipes, the need for more energy to transport oil products, and the waste of energy. For this purpose, the use of an accurate and reliable system for determining the amount of scale inside the pipes has always been one of the needs of the oil industry. In this research, a non-invasive, accurate, and reliable system is presented, which works based on the attenuation of gamma rays. A dual-energy gamma source (241Am and 133Ba radioisotopes), a sodium iodide detector, and a steel pipe are used in the structure of the detection system. The configuration of the detection structure is such that the dual-energy source and the detector are directly opposite each other and on both sides of the steel pipe. In the steel pipe, a stratified flow regime consisting of gas, water, and oil in different volume percentages was simulated using Monte Carlo N Particle (MCNP) code. Seven scale thicknesses between 0 and 3 cm were simulated inside the tube. After the end of the simulation process, the received signals were labeled and transferred to the frequency domain usage of fast Fourier transform (FFT). Frequency domain signals were processed, and four frequency characteristics were extracted from them. The multilayer perceptron (MLP) neural network was used to obtain the relationship between the extracted frequency characteristics and the scale thickness. Frequency characteristics were defined as inputs and scale thickness in cm as the output of the neural network. The prediction of scale thickness with an RMSE of 0.13 and the use of only one detector in the structure of the detection system are among the advantages of this research.
Evaluation Methods of Salt Pillar Stability of Salt Cavern Energy Storage
Authors: Ma Hongling , Wei Xinxing , Shi Xilin , Liang Xiaopeng , Bai Weizheng , Ge Lingzhi
Published: October 2022

Underground energy storage is essential for the country’s development, and underground salt cavern groups are a productive way to store energy. Safety pillar design is the key to ensuring the safe operation of large salt cavern gas storage groups. Therefore, this paper is based on the salt pillar stability design problem and analyzes three aspects: (1) Three kinds of pillar stability design theories—reliability theory, strain energy theory, and catastrophe theory; (2) Two methods for designing stable pillars—determining pillars through formulas and numerical simulations; (3) The form and influencing factors of salt pillar instability—macro and microform and the main influential factors. From the current research, the major problem is that the design of safety pillars has not been systematic, due to the differences in salt rock in the region, and the engineering of salt pillar design still relies on experience. Finally, the impact of salt pillar width and gas injection and withdrawal on the pillars during the design of the salt cavern is analyzed, and the existing salt pillar theoretical characteristics and development trend are summarized.
Myoma with Hypermenorrhea Treated with Ultrasound-Guided Microwave Ablation of the Inflowing Blood Vessels to the Uterine Myoma: A Case
Authors: Kakinuma Toshiyuki , Ohkusa Takahumi , Shinohara Takumi , Shimizu Ayano , Okamoto Rora , Kagimoto Masataka , Kaneko Ayaka , Kakinuma Kaoru , Yanagida Kaoru , Takeshima Nobuhiro , Ohwada Michitaka
Published: October 2022

Microwave endometrial ablation (MEA) is a minimally invasive treatment for uterine myoma with hypermenorrhea, which can replace conventional hysterectomy. However, cases requiring additional treatment because of postoperative recurrence are often encountered. MEA cauterizes the endometrium and is not recommended for patients who wish to preserve fertility. We present the cases of a patient with myoma-related hypermenorrhea who underwent microwave ablation of the inflowing blood vessels to the uterine myoma under transvaginal ultrasound guidance. A 43-year-old woman was diagnosed with chronic myeloid leukemia and treated with dasatinib 2 years ago. Worsening hypermenorrhea was observed after treatment initiation. Ultrasound and pelvic magnetic resonance imaging revealed a uterine myoma. Therefore, she underwent MEA under transvaginal ultrasound guidance. Visual analog scale evaluation demonstrated considerable improvement in hypermenorrhea and dysmenorrhea; the myoma size showed reduction. The postoperative course was uneventful, and the patient was discharged on the day after surgery. No postoperative complications were observed. This patient is currently undergoing infertility treatment. The microwave ablation of myoma under transvaginal ultrasound guidance can effectively and safely reduce the myoma size. These findings suggest that this method is a novel treatment option for patients with myoma-related hypermenorrhea who wish to preserve their fertility and have children.
ACP-ADA: A Boosting Method with Data Augmentation for Improved Prediction of Anticancer Peptides
Authors: Bhattarai Sadik , Kim Kyu-Sik , Tayara Hilal , Chong Kil To
Published: October 2022

Cancer is the second-leading cause of death worldwide, and therapeutic peptides that target and destroy cancer cells have received a great deal of interest in recent years. Traditional wet experiments are expensive and inefficient for identifying novel anticancer peptides; therefore, the development of an effective computational approach is essential to recognize ACP candidates before experimental methods are used. In this study, we proposed an Ada-boosting algorithm with the base learner random forest called ACP-ADA, which integrates binary profile feature, amino acid index, and amino acid composition with a 210-dimensional feature space vector to represent the peptides. Training samples in the feature space were augmented to increase the sample size and further improve the performance of the model in the case of insufficient samples. Furthermore, we used five-fold cross-validation to find model parameters, and the cross-validation results showed that ACP-ADA outperforms existing methods for this feature combination with data augmentation in terms of performance metrics. Specifically, ACP-ADA recorded an average accuracy of 86.4% and a Mathew’s correlation coefficient of 74.01% for dataset ACP740 and 90.83% and 81.65% for dataset ACP240; consequently, it can be a very useful tool in drug development and biomedical research.
Anti-PTK7 Monoclonal Antibodies Exhibit Anti-Tumor Activity at the Cellular Level and in Mouse Xenograft Models of Esophageal Squamous Cell Carcinoma
Authors: Kim Jae Hoon , Shin Won-Sik , Lee Se-Ra , Kim Sanggil , Choi So-Young , Lee Seung-Taek
Published: October 2022

PTK7 is a catalytically defective receptor protein tyrosine kinase upregulated in various cancers, including esophageal squamous cell carcinoma (ESCC). In previous studies, we observed a positive correlation between PTK7 expression levels and tumorigenicity in various ESCC cell lines and xenograft mice with ESCC KYSE-30 cells. In this study, we analyzed the effects of anti-PTK7 monoclonal antibodies (mAbs) on the tumorigenic activity in KYSE-30 cells and in mouse xenograft models. PTK7 mAb-32 and mAb-43 bind with a high affinity to the extracellular domain of PTK7. PTK7 mAbs significantly reduced three-dimensional cell proliferation, adhesion, wound healing, and migration. PTK7 mAbs also reduce chemotactic invasiveness by decreasing MMP-9 secretion. PTK7 mAbs decreased actin cytoskeleton levels in the cortical region of KYSE-30 cells. PTK7 mAbs reduced the phosphorylation of ERK, SRC, and FAK. In a mouse xenograft model of ESCC using KYSE-30 cells, PTK7 mAbs reduced tumor growth in terms of volume, weight, and the number of Ki-67-positive cells. These results demonstrated that PTK7 mAbs can inhibit the tumorigenicity of ESCC at the cellular level and in vivo by blocking the function of PTK7. Considering the anticancer activities of PTK7 mAbs, we propose that PTK7 mAbs can be used in an effective treatment strategy for PTK7-positive malignancies, such as ESCC.
Genetic Mechanism of Tissue-Specific Expression of PPAR Genes in Turbot (Scophthalmus maximus) at Different Temperatures
Authors: Wang Xinan , Zhao Tingting , Ma Aijun
Published: October 2022

In this study, we used PCR to measure the levels of the peroxisome proliferator activated receptor genes PPARα1, PPARα2, PPARβ, and PPARγ in the intestine, liver, gill, heart, kidney, brain, muscle, spleen, skin, and stomach of turbot (Scophthalmus maximus) cultured under different temperature conditions (14, 20, 23, 25, and 28 °C). We used split-split-plot (SSP) analysis of variance, additive main effects and multiplicative interaction (AMMI) analysis, and genotype main effects and genotype × environment interaction (GGE) biplot analysis to evaluate the genotype × tissue interaction effects on gene expression. The results of the SSP analysis of variance showed that temperature and tissue × gene have highly significant (p < 0.01) effect on the expression of S. maximus PPAR genes. The AMMI analysis results revealed that the expression of PPAR genes at the appropriate temperature (14 °C) mainly depended on genotype × tissue interaction and tissue effects. Under stress temperatures, genotype effects, tissue effects, and genotype × tissue interaction, all had significant effects on the expression of PPAR genes. The contribution of the genotype effect slowly increased with increasing temperature; it increased faster at 20 °C and then slowly declined at 25 °C. The contribution of the tissue effect slowly increased from 14 to 20 °C, where it sharply decreased, and then it stabilized after a slight fluctuation. The contribution of the genotype × tissue interaction effect showed a fluctuating upward trend throughout the experiment, and it had a significant impact on PPAR gene expression. The key temperature at which the three effects changed was 20 °C, indicating that it is the limit temperature for active lipid metabolism under high-temperature stress. The GGE biplot analysis results showed that under suitable water temperature, the expression difference of PPAR genes in the liver was the largest; at 20 and 23 °C, the expression difference in the gill was the largest; and at 25 and 28 °C, the expression difference in the brain was the largest. Overall, our results suggest that the mechanism responsible for PPAR gene expression under the three high temperatures (23, 25, and 28 °C) was relatively consistent, but it differed from that at 20 °C.
Influence of Different Salts on the G-Quadruplex Structure Formed from the Reversed Human Telomeric DNA Sequence
Authors: Olejko Lydia , Dutta Anushree , Shahsavar Kosar , Bald Ilko
Published: October 2022

G-rich telomeric DNA plays a major role in the stabilization of chromosomes and can fold into a plethora of different G-quadruplex structures in the presence of mono- and divalent cations. The reversed human telomeric DNA sequence (5′-(GGG ATT)4; RevHumTel) was previously shown to have interesting properties that can be exploited for chemical sensing and as a chemical switch in DNA nanotechnology. Here, we analyze the specific G-quadruplex structures formed by RevHumTel in the presence of K+, Na+, Mg2+ and Ca2+ cations using circular dichroism spectroscopy (CDS) and Förster resonance energy transfer (FRET) based on fluorescence lifetimes. CDS is able to reveal strand and loop orientations, whereas FRET gives information about the distances between the 5′-end and the 3′-end, and also, the number of G-quadruplex species formed. Based on this combined information we derived specific G-quadruplex structures formed from RevHumTel, i.e., a chair-type and a hybrid-type G-quadruplex structure formed in presence of K+, whereas Na+ induces the formation of up to three different G-quadruplexes (a basket-type, a propeller-type and a hybrid-type structure). In the presence of Mg2+ and Ca2+ two different parallel G-quadruplexes are formed (one of which is a propeller-type structure). This study will support the fundamental understanding of the G-quadruplex formation in different environments and a rational design of G-quadruplex-based applications in sensing and nanotechnology.
Wnt Signaling and Aging of the Gastrointestinal Tract
Authors: Asano Naoki , Takeuchi Akio , Imatani Akira , Saito Masashi , Jin Xiaoyi , Hatta Waku , Uno Kaname , Koike Tomoyuki , Masamune Atsushi
Published: October 2022

Aging is considered a risk factor for various diseases including cancers. In this aging society, there is an urgent need to clarify the molecular mechanisms involved in aging. Wnt signaling has been shown to play a crucial role in the maintenance and differentiation of tissue stem cells, and intensive studies have elucidated its pivotal role in the aging of neural and muscle stem cells. However, until recently, such studies on the gastrointestinal tract have been limited. In this review, we discuss recent advances in the study of the role of Wnt signaling in the aging of the gastrointestinal tract and aging-related carcinogenesis.
Engineered Human Intervertebral Disc Model Inducing Degenerative Microglial Proinflammation
Authors: Hwang Min-Ho , Kang You Jung , Son Hyeong-Guk , Cho Hansang , Choi Hyuk
Published: October 2022

Degeneration of the intervertebral disc (IVD) is a major contributor to low back pain (LBP). IVD degeneration is characterized by abnormal production of inflammatory cytokines secreted by IVD cells. Although the underlying molecular mechanisms of LBP have not been elucidated, increasing evidence suggests that LBP is associated particularly with microglia in IVD tissues and the peridiscal space, aggravating the cascade of degenerative events. In this study, we implemented our microfluidic chemotaxis platform to investigate microglial inflammation in response to our reconstituted degenerative IVD models. The IVD models were constructed by stimulating human nucleus pulposus (NP) cells with interleukin-1β and producing interleukin-6 (129.93 folds), interleukin-8 (18.31 folds), C-C motif chemokine ligand-2 (CCL-2) (6.12 folds), and CCL-5 (5.68 folds). We measured microglial chemotaxis (p < 0.05) toward the conditioned media of the IVD models. In addition, we observed considerable activation of neurodegenerative and deactivation of protective microglia via upregulated expression of CD11b (p < 0.001) and down-regulation of CD206 protein (p < 0.001) by soluble factors from IVD models. This, in turn, enhances the inflammatory milieu in IVD tissues, causing matrix degradation and cellular damage. Our findings indicate that degenerative IVD may induce degenerative microglial proinflammation, leading to LBP development.
Polyclonal Aptamer Libraries from a FluRoot-SELEX for the Specific Labeling of the Apical and Elongation/Differentiation Zones of Arabidopsis thaliana Roots
Authors: Kissmann Ann-Kathrin , Wolf Dennis , Krämer Markus , Müller Franziska , Amann Valerie , Xing Hu , Gottschalk Kay-Eberhard , Weil Tanja , Eichmann Ruth , Schäfer Patrick , Rosenau Frank
Published: October 2022

In more than 30 years of aptamer research, it has become widely accepted that aptamers are fascinating binding molecules for a vast variety of applications. However, the majority of targets have been proteins, although special variants of the so-called SELEX process for the molecular evolution of specific aptamers have also been developed, allowing for the targeting of small molecules as well as larger structures such as cells and even cellular networks of human (tumor) tissues. Although the provocative thesis is widely accepted in the field, that is, in principle, any level of complexity for SELEX targets is possible, the number of studies on whole organs or at least parts of them is limited. To pioneer this thesis, and based on our FluCell-SELEX process, here, we have developed polyclonal aptamer libraries against apices and the elongation/differentiation zones of plant roots as examples of organs. We show that dedicated libraries can specifically label the respective parts of the root, allowing us to distinguish them in fluorescence microscopy. We consider this achievement to be an initial but important evidence for the robustness of this SELEX variant. These libraries may be valuable tools for plant research and a promising starting point for the isolation of more specific individual aptamers directed against root-specific epitopes.
Mineral Bone Disorders in Kidney Disease Patients: The Ever-Current Topic
Authors: Hu Lilio , Napoletano Angelodaniele , Provenzano Michele , Garofalo Carlo , Bini Claudia , Comai Giorgia , La Manna Gaetano
Published: October 2022

Chronic kidney disease (CKD) is a complex and multifactorial disease, and one of the most prevalent worldwide. Chronic kidney disease–mineral bone disorders (CKD–MBD) with biochemical and hormonal alterations are part of the complications associated with the progression of CKD. Pathophysiology of CKD–MBD focused on abnormalities in serum levels of several biomarkers (such as FGF-23, klotho, phosphate, calcium, vitamin D, and PTH) which are discussed in this review. We therefore examine the prognostic association between CKD–MBD and the increased risk for cardiovascular events, mortality, and CKD progression to end-stage kidney disease (ESKD). Lastly, we present specific treatments acting on CKD to prevent and treat the complications associated with secondary hyperparathyroidism (SHPT): control of hyperphosphatemia (with dietary restriction, intestinal phosphate binders, and adequate dialysis), the use of calcimimetic agents, vitamin D, and analogues, and the use of bisphosphonates or denosumab in patients with osteoporosis.
Prolonged Febrile Seizures Impair Synaptic Plasticity and Alter Developmental Pattern of Glial Fibrillary Acidic Protein (GFAP)-Immunoreactive Astrocytes in the Hippocampus of Young Rats
Authors: Griflyuk Alexandra V. , Postnikova Tatyana Y. , Zaitsev Aleksey V.
Published: October 2022

Prolonged neonatal febrile seizures (FSs) often lead to cognitive decline and increased risk of psychopathology in adulthood. However, the neurobiological mechanisms underlying the long-term adverse effects of FSs remain unclear. In this study, we exposed rat pups to hyperthermia and induced FSs lasting at least 15 min. We investigated the short-term (one day) and delayed (11–13 and 41–45 days) effects of FSs on some parameters of morphological and functional maturation in the hippocampus. We noticed that FSs altered the developmental pattern of glial fibrillary acidic protein (GFAP) immunoreactivity. In rats aged 21–23 days, GFAP-positive astrocytes covered a smaller area, and their morphological characteristics resembled those of rats at 11 days of age. In post-FS rats, the magnitude of long-term synaptic potentiation was reduced compared to control animals of the same age. Applying the gliotransmitter D-serine, an agonist of the glycine site of NMDA receptors, restored LTP to control values. A decrease in LTP amplitude was correlated with impaired spatial learning and memory in the Barnes maze task in post-FS rats. Our data suggest that impaired neuron–glia interactions may be an essential mechanism of the adverse effects of FS on the developing brain.
Granulation of Bismuth Oxide by Alginate for Efficient Removal of Iodide in Water
Authors: Kim Tae-Hyun , Seo Chihyun , Seon Jaeyoung , Battulga Anujin , Hwang Yuhoon
Published: October 2022

The granulation of bismuth oxide (BO) by alginate (Alg) and the iodide adsorption efficacy of Alg–BO for different initial iodide concentrations and contact time values were examined. The optimal conditions for Alg–BO granulation were identified by controlling the weight ratio between Alg and BO. According to the batch iodide adsorption experiment, the Alg:BO weight ratio of 1:20 was appropriate, as it yielded a uniform spherical shape. According to iodide adsorption isotherm experiments and isotherm model fitting, the maximum sorption capacity (qm) was calculated to be 111.8 mg/g based on the Langmuir isotherm, and this value did not plateau even at an initial iodide concentration of 1000 mg/L. Furthermore, iodide adsorption by Alg–BO occurred as monolayer adsorption by the chemical interaction and precipitation between bismuth and iodide, followed by physical multilayer adsorption at a very high concentration of iodide in solution. The iodide adsorption over time was fitted using the intraparticle diffusion model. The results indicated that iodide adsorption was proceeded by boundary layer diffusion during 480 min and reached the plateau from 1440 min to 5760 min by intraparticle diffusion. According to the images obtained using cross-section scanning electron microscopy assisted by energy-dispersive spectroscopy, the adsorbed iodide interacted with the BO in Alg–BO through Bi–O–I complexation. This research shows that Alg–BO is a promising iodide adsorbent owing to its high adsorption capacity, stability, convenience, and ability to prevent secondary pollution.
Repurposing Alone and in Combination of the Antiviral Saquinavir with 5-Fluorouracil in Prostate and Lung Cancer Cells
Authors: Pereira Mariana , Vale Nuno
Published: October 2022

Prostate and lung cancers are among the most common cancer types, and they still need more therapeutics. For this purpose, saquinavir (SAQ) was tested alone and in combination with 5-fluorouracil (5-FU). PC-3 and A549 cells were exposed to increasing concentrations of both drugs alone or in combination, with simultaneous or sequential administration. Cell viability was obtained using the MTT assay and synergism values using CompuSyn software. Results showed that SAQ was the more cytotoxic of both drugs in PC-3 cells, while 5-FU was the most cytotoxic in A549 cells. When these drugs were used in combination, the more synergistic combination in PC-3 cells was the IC50 of SAQ with various concentrations of 5-FU, particularly when 5-FU was only applied 24 h later. Meanwhile for A549 the most promising combination was 5-FU with delayed SAQ, but with a weaker effect than all combinations demonstrated in PC-3 cells. These results demonstrate that SAQ could be used as a new repurposed drug for the treatment of prostate cancer and this treatment potential could be even greater if SAQ is combined with the anticancer drug 5-FU, while for lung cancer it is not as efficient and, therefore, not of as much interest.
Transcriptional Responses of Copper-Transport-Related Genes ctr1, ctr2 and atox1 and Their Roles in the Regulation of Cu Homeostasis in Yellow Catfish Pelteobagrus fulvidraco
Authors: Yang Hong , Zhong Chongchao , Tan Xiaoying , Chen Guanghui , He Yang , Liu Shengzan , Luo Zhi
Published: October 2022

Here, we characterized the function of ctr1, ctr2 and atox1 promoters in yellow catfish Pelteobagrus fulvidraco, a common freshwater teleost in Asian countries. We obtained 1359 bp, 1842 bp and 1825 bp sequences of ctr1, ctr2 and atox1 promoters, and predicted key transcription factor binding sites on their promoters, including MRE, SREBP1, NRF2, KLF4 and STAT3. Cu differentially influenced the activities of ctr1, ctr2 and atox1 promoters from different regions. We found that the −326/−334 bp and −1232/−1240 bp locus in the atox1 promoter were functional NRF2 binding sites, which negatively controlled the activity of the atox1 promoter. The −91/−100 bp locus in the ctr1 promoter and −232/−241 bp and −699/−708 bp locus in the atox1 promoter were functional SREBP1 binding sites, which positively controlled the activities of ctr1 and atox1 promoters. Cu inhibited the NRF2 binding ability to the atox1 promoter, but promoted the SREBP1 binding ability to the ctr1 and atox1 promoters. Dietary Cu excess significantly down-regulated hepatic mRNA and total protein expression of CTR1, CTR2 and ATOX1 of yellow catfish, compared to the adequate dietary Cu group. The subcellular localization showed that CTR1 was mainly localized on the cell membrane, CTR2 in the cell membrane and the lysosome, and ATOX1 in the cytoplasm. In conclusion, we demonstrated the regulatory mechanism of three Cu transporters at the transcription levels, and found the functional NRF2 and SREBP1 response elements in ctr1, ctr2 and atox1 promoters, which provided new insights into their roles in the regulation of Cu homeostasis in fish.
Reduction of Thoracic Hyper-Kyphosis Improves Short and Long Term Outcomes in Patients with Chronic Nonspecific Neck Pain: A Randomized Controlled Trial
Authors: Moustafa Ibrahim Moustafa , Shousha Tamer Mohamed , Walton Lori M. , Raigangar Veena , Harrison Deed E.
Published: October 2022

This study investigates thoracic hyper kyphosis (THK) rehabilitation using the Denneroll™ thoracic traction orthosis (DTTO). Eighty participants, with chronic non-specific neck pain (CNSNP) and THK were randomly assigned to the control or intervention group (IG). Both groups received the multimodal program; IG received the DTTO. Outcomes included formetric thoracic kyphotic angle ICT—ITL, neck pain and disability (NDI), head repositioning accuracy (HRA), smooth pursuit neck torsion test (SPNT) and overall stability index (OSI). Measures were assessed at baseline, after 30 treatment sessions over the course of 10 weeks, and 1-year after cessation of treatment. After 10 weeks, the IG improved more in neck pain intensity (p < 0.0001) and NDI (p < 0.001). No differences were found for SPNT (p = 0.48) and left-sided HRA (p = 0.3). IG improved greater for OSI (p = 0.047) and right sided HRA (p = 0.02). Only the IG improved in THK (p < 0.001). At 1-year follow-up, a regression back to baseline values for the control group was found for pain and disability such that all outcomes favored improvement in the IG receiving the DTTO; all outcomes (p < 0.001). The addition of the DTTO to a multimodal program positively affected CNSNP outcomes at both the short and 1-year follow-up.
Sex Differences in the Correlation between Fatigue Perception and Regional Gray Matter Volume in Healthy Adults: A Large-Scale Study
Authors: Putra Handityo Aulia , Park Kaechang , Yamashita Fumio
Published: October 2022

The relationship between fatigue perception and regional gray matter volume (rGMV) has seldom been studied in healthy adults. Therefore, this study aimed to analyze sex differences in the correlation between rGMV and fatigue perception using Chalder’s fatigue questionnaire (CFQ). The CFQ was used to analyze the sexual features of rGMV related to the degree of perceived fatigue in 2955 healthy adults (male = 1560, female = 1395) of various ages (20–89 years, median 56). A higher CFQ score denotes a higher perceived fatigue level by the participant. According to the CFQ scores in males, the volumes of the right orbital part of the inferior frontal gyrus and left precuneus were negatively correlated (i.e., smaller rGMV had a higher CFQ score), whereas the left angular gyrus was positively correlated. In females, the right inferior temporal gyrus was negative, whereas the left middle temporal gyrus and right putamen were positive (i.e., larger rGMV had a higher CFQ score). The lack of identified regions in this large-scale study between males and females might be related to sex differences in clinical or pathological fatigue morbidities. Additionally, the sex differences in the negative or positive correlations between rGMV and fatigue perception may contribute to a better understanding of the neuronal mechanism in the early stages of fatigue development.
Mitral Valve Systolic Anterior Motion in Robotic Thoracic Surgery as the Cause of Unexplained Hemodynamic Shock: From a Case Report to Recommendations
Authors: Monaco Fabrizio , D’Amico Filippo , Barucco Gaia , Licheri Margherita , Novellis Pierluigi , Ciriaco Paola , Veronesi Giulia
Published: October 2022

Robotic major lung resection for lung cancer carries a risk for intraoperative hemodynamic instability. Systolic anterior motion (SAM) of the mitral valve is a rare and often misrecognized cause of intraoperative hemodynamic instability. If not promptly recognized, SAM leads to a complicated perioperative course. Here, we report for the first time a case of a patient with SAM with a severe degree of left ventricular outflow obstruction (LVOTO) undergoing robotic lung lobectomy and its challenging intraoperative management. A 70-year-old man undergoing robotic left upper lobectomy developed immediately after the induction of general anesthesia hemodynamic instability due to SAM-related LVOTO. The diagnosis was possible, thanks to the use of transesophageal echocardiography (TEE). The treatment strategies applied were preload optimization without fluid overload, ultra-short-acting beta-blockers, and vasopressors. Peripheral nerve blockades were preferred over epidural analgesia to avoid vasodilatation. The patient reported a good quality of recovery and no pain the day after surgery. The management of patients with higher risk of SAM and LVOTO development during robotic thoracic surgery requires a dedicated and skilled team together with high-impact treatment strategies driven by TEE. Since current guidelines do not recommend the use of TEE, even for patients with higher cardiac risk undergoing noncardiac surgery, the present case report may stimulate interest in future recommendations.
Risk Factors Associated with Severe Disease and Intensive Care Unit Admission of Pregnant Patients with COVID-19 Infection—A Retrospective Study
Authors: Adam Ana-Maria , Vasilache Ingrid-Andrada , Socolov Demetra , Stuparu Cretu Mariana , Georgescu Costinela Valerica , Vicoveanu Petronela , Mihalceanu Elena , Harabor Anamaria , Socolov Razvan
Published: October 2022

(1) Background: Pregnant patients with severe forms of coronavirus disease 2019 (COVID-19) can experience adverse pregnancy outcomes. The aim of this study was to retrospectively assess the risk factors associated with admission to the intensive care unit (ICU) of pregnant patients with COVID-19, as well as the pregnancy outcomes of these patients; (2) Methods: Medical records of 31 pregnant patients with COVID-19 admitted to three clinical hospitals from Romania, between October 2020 and November 2021 were examined. The patients were segregated into two groups depending on their clinical evolution: non-ICU admission (n = 19) or ICU admission (n = 12). Clinical and paraclinical findings were evaluated using univariate analysis, and the association of significant risk factors with maternal ICU admission was assessed using a multivariate analysis. Pregnancy outcomes of these patients were also recorded; (3) Results: Pulmonary disease, cough, dyspnea, leukocytosis, thrombocytosis, high serum values of transaminases, serum ferritin, and increased duration of hospital admission were identified as significant risk factors associated with maternal admission to the ICU. No significant differences regarding pregnancy outcomes were noted between the evaluated patients; (4) Conclusions: Specific risk factor identification in pregnant patients with severe forms of COVID-19 could improve the patient’s management.
Body Composition, Fasting Blood Glucose and Lipidemic Indices Are Not Primarily Determined by the Nutritional Intake of Middle-Aged Endurance Trained Men—Another “Athletes’ Paradox”?
Authors: Methenitis Spyridon , Feidantsis Konstantinos , Kaprara Athina , Hatzitolios Apostolos , Skepastianos Petros , Papadopoulou Sousana K. , Panayiotou George
Published: October 2022

Systematic, regular high-volume endurance training induces significant metabolic adaptations in glucose and lipids metabolism, which seems to affect the negative impact of unhealthy nutrition, at least in animal models. The present study aimed to investigate the main determinants of body composition, blood glucose and lipids concentrations between middle-aged sedentary individuals (Sed) and well-trained endurance athletes (Run), both following an unhealthy high-fat diet. In thirty-five Sed (Age: 54.0 ± 6.6 yrs, Body Mass: 77.1 ± 10.5 kg, BMI: 31.3 ± 6.0 kg·m−2) and thirty-six Run (Age: 51.6 ± 5.2 yrs, Body Mass: 85.8 ± 3.4 kg, BMI: 23.2 ± 1.8 kg·m−2), body composition, nutritional intake, energy expenditure, resting metabolic rate (RMR), respiratory exchange ratio (RER), and blood glucose and lipids concentrations were evaluated. Multiple linear regression analyses revealed that body composition, blood glucose and lipids’ concentrations in the Run group were primarily determined by the energy expenditure (B: −0.879 to −1.254), while in the Sed group, by their energy intake (B:−0.754 to 0.724). In conclusion, it seems that in well-trained endurance middle-aged athletes, body composition, blood glucose, and lipids concentrations seem to be determined by their training-induced daily energy expenditure and not by their nutritional intake per se. At the same time, nutrition is the primary determinant in aged-matched sedentary individuals, even if they both follow high-fat diets.
Custom-Made 3D-Printed Implants for Anterior Column Reconstruction in the Upper Cervical Spine after Intralesional Extracapsular Excision—Report of 2 Cases and Literature Review
Authors: Girolami Marco , Griffoni Cristiana , Asunis Emanuela , Falzetti Luigi , Bandiera Stefano , Barbanti Brodano Giovanni , Ghermandi Riccardo , Pipola Valerio , Terzi Silvia , Pesce Eleonora , Monopoli Forleo Donato , Cianchetti Marco , Fiore Maria Rosaria , Presutti Livio , Fini Milena , Gasbarrini Alessandro
Published: October 2022

The use of three-dimensional (3D)-printed custom-made implants is spreading in the orthopedics field for the reconstruction of bone losses or for joint replacement, thanks to their unparalleled versatility. In particular, this novel technology opens new perspectives to formulate custom-made fixation strategies for the upper cervical region, sacrum and pelvis, where reconstruction is challenging. We report and analyze the literature concerning upper cervical reconstruction with 3D-printed personalized implants after tumor surgery, and discuss two cases of patients where this technology was used to reconstruct the anterior column after extracapsular debulking of C2 recurrent chordoma at our institution.
Diversity and Distribution of Macrofungi in Protected Mountain Forest Habitats in Serbia and Its Relation to Abiotic Factors
Authors: Rakić Milana , Marković Miroslav , Galić Zoran , Galović Vladislava , Karaman Maja
Published: October 2022

Fungal diversity is one of the most important indicators of overall forest biodiversity and its health. However, scarce information exists on the state of macrofungal communities of mountain forests in Serbia, making it one of the countries with the least-published mycological data in the Mediterranean and Balkan region of Europe. This paper presents the results of the first comprehensive, long-term study of macrofungal communities in some of the most important mountain forest ecosystems in Serbia (Tara, Kopaonik and Vidlič). In the course of three consecutive years, the sampling of five permanent experimental plots resulted in 245 species of macrofungi, classified into three functional groups (terricolous saprothrophs, lignicolous, and mycorrhizal fungi). Special attention was given to protected and indicator species, which point out the great value of studied forest habitats and the importance of their conservation. It was found that precipitation, habitat humidity, and temperature significantly influence the occurrence and distribution, primarily of mycorrhizal and lignicolous group of fungi. Thus, the continuation of long-term monitoring is crucial in order to more precisely determine which groups/species of macrofungi would, and to what extent they would, adapt to a rapidly changing climate.
Regulator of G Protein Signaling Proteins Control Growth, Development and Cellulase Production in Neurospora crassa
Authors: Cabrera Ilva E. , Oza Yagna , Carrillo Alexander J. , Collier Logan A. , Wright Sara J. , Li Liande , Borkovich Katherine A.
Published: October 2022

Heterotrimeric (αβγ) G protein signaling pathways are critical environmental sensing systems found in eukaryotic cells. Exchange of GDP for GTP on the Gα subunit leads to its activation. In contrast, GTP hydrolysis on the Gα is accelerated by Regulator of G protein Signaling (RGS) proteins, resulting in a return to the GDP-bound, inactive state. Here, we analyzed growth, development and extracellular cellulase production in strains with knockout mutations in the seven identified RGS genes (rgs-1 to rgs-7) in the filamentous fungus, Neurospora crassa. We compared phenotypes to those of strains with either knockout mutations or expressing predicted constitutively activated, GTPase-deficient alleles for each of the three Gα subunit genes (gna-1Q204L, gna-2Q205L or gna-3Q208L). Our data revealed that six RGS mutants have taller aerial hyphae than wild type and all seven mutants exhibit reduced asexual sporulation, phenotypes shared with strains expressing the gna-1Q204L or gna-3Q208L allele. In contrast, Δrgs-1 and Δrgs-3 were the only RGS mutants with a slower growth rate phenotype, a defect in common with gna-1Q204L strains. With respect to female sexual development, Δrgs-1 possessed defects most similar to gna-3Q208L strains, while those of Δrgs-2 mutants resembled strains expressing the gna-1Q204L allele. Finally, we observed that four of the seven RGS mutants had significantly different extracellular cellulase levels relative to wild type. Of interest, the Δrgs-2 mutant had no detectable activity, similar to the gna-3Q208L strain. In contrast, the Δrgs-1 and Δrgs-4 mutants and gna-1Q204L and gna-2Q205L strains exhibited significantly higher cellulase activity than wild type. With the exception of sexual development, our results demonstrate the greatest number of genetic interactions between rgs-1 and gna-1 and rgs-2 and gna-3 in N. crassa.
Impaired Fibrinolytic Potential Predicts Oxygen Requirement in COVID-19
Authors: Wang Julie , Choy Kay Weng , Lim Hui Yin , Ho Prahlad
Published: October 2022

Abnormal coagulation and fibrinolysis contributes to the respiratory distress syndrome in COVID-19. We aimed to explore the association of impaired fibrinolytic potential with disease severity and oxygen requirement in hospitalized patients. Adults admitted to hospital with confirmed COVID-19 infection between 1–31 January 2022 were included, corresponding to the first Omicron outbreak in Melbourne, Victoria. The first citrated plasma sample requested within 24 h of the patient’s presentation was obtained and analyzed by the overall hemostatic potential (OHP) assay, a spectrophotometric assay in which fibrin formation (triggered by small amounts of thrombin (OCP)) and fibrinolysis (by the addition of thrombin and tissue plasminogen activator (OHP and OFP%)) were simultaneously measured. There were 266 patients (median 72 years, 52.9% male), of which 49.6% did not require oxygen therapy. COVID-19 severity and requirement for oxygen was significantly associated with higher OCP, OHP, and lower OFP%. Vaccinated individuals compared with non-vaccinated individuals had significantly lower OHP (16.5 vs. 23.1, p = 0.015) and higher OFP (72.0% vs. 65.1%, p = 0.005), as well as significantly lower AST, ferritin, LDH, CRP, and D-dimer. A multivariate model containing OHP was constructed with the outcome of oxygen requirement, with c-statistic of 0.85 (95%CI 0.81–0.90). In this pilot study, we show a significant correlation between OHP results and requirement for oxygen supplementation in hospitalized patients during a period dominated by the Omicron variant. The results were incorporated into a multivariate model that predicted for oxygen requirement, with high discriminative ability.
Choice of the Optimal Dialysate Sodium Concentration
Authors: McIntyre Christopher W.
Published: October 2022

The choice of dialysate sodium concentration remains amongst the most crucial and difficult to address challenges, in the care of hemodialysis (HD) patients. Our understanding of the determinants of sodium transport, as well as the consequences of getting the decisions wrong, remains both imperfect and evolving. This question has been subject to far less study than it deserves. In this short piece we consider what we are trying to achieve with dialysate sodium choices and how best to individualize those choices to address the symptomatic and survival-based needs of our patients.
Coupling Efficiency Assessment of Food–Energy–Water (FEW) Nexus Based on Urban Resource Consumption towards Economic Development: The Case of Shenzhen Megacity, China
Authors: Xian Chaofan , Yang Shuo , Fan Yupeng , Wu Haotong , Gong Cheng
Published: October 2022

The population aggregation and economic development caused by urbanization significantly influence the efficiency of urban resource consumption. However, the coupling interactions between crucial resource consumptions such as food, energy and water (FEW) and urbanization processes within highly urbanized areas has not been well-studied. In this study, we constructed an assessment framework for the coupling efficiency measurement of FEW resource consumptions in 10 administrative districts across Shenzhen megacity during 2012–2020, based on the data envelopment analysis (DEA). This study demonstrated that, from the perspective of the FEW nexus, increasing efficiencies in the energy consumption of most districts improved the municipal FEW efficiency, while more than half of the districts did not achieve water resource efficiencies throughout the period. Concerning regional economic development, 80% of the districts improved coupling FEW efficiencies by 2020, the average values of which were higher for Yantian, Nanshan, Luohu and Dapeng, and lower for Baoan, Longgang and Guangming, with a downtrend only being observed in Guangming. Overall, the value of the coupling FEW efficiency of Shenzhen megacity rose by 35% from 2012 to 2020. Correlation analysis showed that synergistic effects of efficient resource consumption occurred in most districts, and economic urbanization was the main driving factor of regional FEW efficiencies within Shenzhen megacity. This study provides instructive insights into the status of urban resource consumption and suggests that the coordination of FEW management should be further improved by fiscal intervention to maintain economic development with the limited resources available, which would have valuable implications for synergistic FEW governance in megacities in China and elsewhere.
Assessing the Spatial Variability of Daytime/Nighttime Extreme Heat Waves in Beijing under Different Land-Use during 2011–2020
Authors: Su Xiaokang , Wang Fang , Zhou Demin , Zhang Hongwen
Published: October 2022

Urban land-use affects surface air temperature; however, the impact of urban land-use on surface air temperature, particularly the extent to which it affects the duration of extreme heat waves, remains uncertain and the mechanisms of diurnal differences need to be further explored. This paper presents study of daytime/nighttime extreme heat waves duration in Beijing under different land-use changes by adopting an index of cumulative hours of extreme heat waves exceeding the certain thresholds. The urban day/night extreme heat waves cumulative hourly interpolation models were established based on high-resolution urban land-use and socioeconomic data and were assessed to have good performance. The annual average cumulative hours of extreme heat waves increased by 95% (daytime) and 116% (nighttime) in 2016–2020 compared to 2011–2015. The cumulative hours for each land-use type ranked as follows: urban land > cropland > water > grassland > woodland. We found that the cumulative hours of extreme heat waves increased significantly with the proportion of urban land and decreased significantly with the proportion of forested land and water. This research provided important information for alleviating extreme heat waves in cities and for rational land planning.
Experimental Investigation into the Friction Coefficient of Ball-on-Disc in Dry Sliding Contact Considering the Effects of Surface Roughness, Low Rotation Speed, and Light Normal Load
Authors: Wen Qi , Liu Mingming , Zhang Zenglei , Sun Yunyun
Published: October 2022

The friction coefficient is one of the key parameters in the tribological performance of mechanical systems. In the condition of light normal load and low rotation speed, the friction coefficients of ball-on-disc with rough surface in dry sliding contact are experimentally investigated. Friction tests are carried out under normal load 2–9 N, rotation speed 20–48 rpm at room temperature, and surface roughness 0.245–1.010 μm produced by grinding, milling, and turning. Results show that the friction coefficient increases first and then becomes stable, in which the running-in and steady-state periods are included. With the growth of normal load and rotation speed, or the decline of surface roughness, the duration and fluctuation of the running-in period verge to reduce. The whole rising slope of the friction coefficient in the running-in period goes up more quickly with the increment of rotation speed, and it ascends more slowly as normal load enlarges. In terms of the steady-state period, the deviation of the friction coefficient shows a dwindling trend when normal load or rotation speed grows, or surface roughness descends. As normal load or rotation speed rises, the value of the friction coefficient rises first and then drops. Additionally, the mean value of the friction coefficient in steady-state is approximately independent of surface roughness.
Phosphate Ceramics with Silver Nanoparticles for Electromagnetic Shielding Applications
Authors: Palaimiene Edita , Macutkevič Jan , Banys Jūras , Selskis Algirdas , Apanasevich Natalia , Kudlash Alexander , Sokal Aliaksei , Lapko Konstantin
Published: October 2022

Ceramic composites with nanoparticles are intensively investigated due to their unique thermal, mechanic and electromagnetic properties. In this work, dielectric properties of phosphate ceramics with round silver nanoparticles of various sizes were studied in the wide frequency range of 20 Hz–40 GHz for microwave shielding applications. The percolation threshold in ceramics is close to 30 wt.% of Ag nanoparticles content and it is higher for bigger-sized nanoparticles. The microwave complex dielectric permittivity of ceramics above the percolation threshold is rather high (ε′ = 10 and ε″ = 10 at 30 GHz for ceramics with 50 wt.% inclusions of 30–50 nm size, it corresponds to almost 61% absorption of 2 mm-thickness plate) therefore these ceramics are suitable for microwave shielding applications. Moreover, the microwave absorption is bigger for ceramics with a larger concentration of fillers. In addition, it was demonstrated that the electrical transport in ceramics is thermally activated above room temperature and the potential barrier is almost independent of the concentration of nanoparticles. At very low temperature, the electrical transport in ceramics can be related to electron tunneling.