bluuebunny/crossref_metadata_2025 huggingface dataset:
A J-Type Air-Cooled Battery Thermal Management System Design and Optimization Based on the Electro-Thermal Coupled Model
Authors: Fan Hao , Wang Lan , Chen Wei , Liu Bin , Wang Pengxin
Published: August 2023

Air-cooled battery thermal management system (BTMS) is a widely adopted temperature control strategy for lithium-ion batteries. However, a battery pack with this type of BTMS typically suffers from high temperatures and large temperature differences (∆T). To address this issue, this study conducted an electro-thermal coupled model to optimize the flow channel structure for reducing the maximum temperature (Tmax) and ∆T in a battery pack for a “J-type” air-cooled BTMS. The parameters required to predict battery heat generation were obtained from a single battery testing experiment. The flow and heat transfer model in a battery pack that had 24 18650 batteries was established by the Computational Fluid Dynamics software ANSYS Fluent 2020R2. The simulation results were validated by the measurement from the battery testing experiment. Using the proposed model, parameter analysis has been implemented. The flow channel structure was optimized in terms of the duct size, battery spacing, and battery arrangement for the air-cooled BTMS. The original BTMS was optimized to reduce Tmax and ∆T by 1.57 K and 0.80 K, respectively. This study may provide a valuable reference for designing air-cooled BTMS.
Wind Resource Assessment over the Hellenic Seas Using Dynamical Downscaling Techniques and Meteorological Station Observations
Authors: Kozyrakis Georgios V. , Condaxakis Constantinos , Parasyris Antonios , Kampanis Nikolaos A.
Published: August 2023

The current work focuses on establishing the parameters that influence the wind’s behavior over the Aegean and Ionian Seas and estimating the wind potential in the region based on long-term historic climate data. Combining a downscaling technique performed with the well-founded WRF-ARW computational algorithm and a number of simultaneous meteorological station time series, an attempt is made to investigate how regional changes may affect low-altitude wind speed distribution at hub height (100 m a.s.l.). The provided time-series coastal data span the entire region of interest from north to south. WRF-ARW v.3.9 is utilized to associate the geostrophic wind distribution obtained from long-term Copernicus ERA5 wind data with the localized wind potential over lower altitudes. Evaluation and correlation of the observational data to the predicted wind climate are performed, and the statistical differences that arise are investigated. High-accuracy wind resource potential maps are thus obtained in the region. Also, a few distinctive flow patterns are identified, such as wind speed cut-off regions and very high wind speed distributions, which are presented in specific southern regions of the Aegean Sea.
Suitability Analysis of the Deformation Behavior of Metal Corrugated Casing in High-Temperature Wellbore
Authors: Ren Siqi , Zhao Jing , Kang Zhiqin , Wang Guoying , Yang Dong
Published: August 2023

The stability of the casing is a crucial prerequisite for implementing the in situ high-temperature steam injection method in oil shale reservoirs. In order to address the issues of substantial expansion, concentrated thermal stresses, and susceptibility to damage observed in traditional straight casings under high temperatures, this paper proposes the utilization of a corrugated casing structure. In this regard, to investigate the impact of the shape and structure of the wellbore casing on its mechanical properties, identical corrugated and straight casings were selected and studied. Uniaxial compression and tensile tests were conducted on the casings, along with coordination deformation experiments between the casing and cement sheath under varying temperatures. Numerical simulations were employed to obtain the deformation characteristics of the corrugated and straight casings under axial compression and tension loads, as well as the stress distribution on the outer casing wall. The results showed that when subjected to the same amount of deformation under axial loading, the corrugated casing experienced lower compressive and tensile loads compared to the straight casing. Moreover, under the sole constraint of cement sheath, increasing the temperature led to lower vertical strains (perpendicular to the ground) at all measuring points of the corrugated casing as compared to the corresponding strains in the straight casing. Numerical simulations revealed that, under the same temperatures, the deformation at the interface between the corrugated casing and the cement sheath was smaller, while the vertical stress at the interface of the corrugated casing was also lower than the straight casing. Overall, the corrugated casing, with its corrugated structure that enabled micro-deformation, effectively mitigated the axial deformation of the casing caused by thermal expansion. Consequently, the corrugated casing reduced the extrusion of wellbore casing on the cement sheath, thereby preserving the integrity and stability of the wellbore cementing structure.
Study on the Effects of the Hydrogen Substitution Rate on the Performance of a Hydrogen–Diesel Dual-Fuel Engine under Different Loads
Authors: Liu Xiaole , Liu Shaohua , Shen Lizhong , Bi Yuhua , Duan Longjin
Published: August 2023

Due to having zero carbon emissions and renewable advantages, hydrogen has great prospects as a renewable form of alternate energy. Engine load and hydrogen substitution rate have a considerable influence on a hydrogen–diesel dual-fuel engine’s efficiency. This experiment’s objective is to study the influence of hydrogen substitution rate on engine combustion and emission under different loads and to study the impact of exhaust gas recirculation (EGR) technology or main injection timing on the engine’s capability under high load and high hydrogen substitution rate. The range of the maximum hydrogen substitution rate was determined under different loads (30%~90%) at 1800 rpm and, then, the effects of the EGR rate (0%~15%) and main injection timing (−8 °CA ATDC~0 °CA ATDC) on the engine performance under 90% high load were studied. The research results show that the larger the load, the smaller the maximum hydrogen substitution rate that can be added to the dual-fuel engine. Under each load, with the increase of the hydrogen substitution rate, the cylinder pressure and the peak heat release rate (HRR) increase, the equivalent brake-specific fuel consumption (BSFCequ) decreases, the thermal efficiency increases, the maximum thermal efficiency is 43.1%, the carbon dioxide (CO2) emission is effectively reduced by 35.2%, and the nitrogen oxide (NOx) emission decreases at medium and low loads, and the maximum increase rate is 20.1% at 90% load. Under high load, with the increase of EGR rate or the delay of main injection timing, the problem of NOx emission increases after hydrogen doping can be effectively solved. As the EGR rate rises from 0% to 15%, the maximum reduction of NOx is 63.1% and, with the delay of main injection timing from −8 °CA ATDC to 0 °CA ATDC, the maximum reduction of NOx is 44.5%.
Optimal Power Flow in Distribution Network: A Review on Problem Formulation and Optimization Methods
Authors: Yang Cheng , Sun Yupeng , Zou Yujie , Zheng Fei , Liu Shuangyu , Zhao Bochao , Wu Ming , Cui Haoyang
Published: August 2023

Distributed generators (DGs) have a high penetration rate in distribution networks (DNs). Understanding their impact on a DN is essential for achieving optimal power flow (OPF). Various DG models, such as stochastic and forecasting models, have been established and are used for OPF. While conventional OPF aims to minimize operational costs or power loss, the “Dual-Carbon” target has led to the inclusion of carbon emission reduction objectives. Additionally, state-of-the-art optimization techniques such as machine learning (ML) are being employed for OPF. However, most current research focuses on optimization methods rather than the problem formulation of the OPF. The purpose of this paper is to provide a comprehensive understanding of the OPF problem and to propose potential solutions. By delving into the problem formulation and different optimization techniques, selecting appropriate solutions for real-world OPF problems becomes easier. Furthermore, this paper provides a comprehensive overview of prospective advancements and conducts a comparative analysis of the diverse methodologies employed in the field of optimal power flow (OPF). While mathematical methods provide accurate solutions, their complexity may pose challenges. On the other hand, heuristic algorithms exhibit robustness but may not ensure global optimality. Additionally, machine learning techniques exhibit proficiency in processing extensive datasets, yet they necessitate substantial data and may have limited interpretability. Finally, this paper concludes by presenting prospects for future research directions in OPF, including expanding upon the uncertain nature of DGs, the integration of power markets, and distributed optimization. The main objective of this review is to provide a comprehensive understanding of the impact of DGs in DN on OPF. The article aims to explore the problem formulation of OPF and to propose potential solutions. By gaining in-depth insight into the problem formulation and different optimization techniques, optimal and sustainable power flow in a distribution network can be achieved, leading to a more efficient, reliable, and cost-effective power system. This offers tremendous benefits to both researchers and practitioners seeking to optimize power system operations.
A Comprehensive Analysis of Renewable Energy Based on Integrating Economic Cybernetics and the Autoregressive Distributed Lag Model—The Case of Romania
Authors: Androniceanu Armenia , Georgescu Irina , Nica Ionuț , Chiriță Nora
Published: August 2023

Renewable energy represents a crucial resource in the efforts to combat climate change and reduce dependence on fossil fuels. In the past few decades, Romania has lessened its environmental footprint and played an important role in actions against climate change. In this research, the energy sector in Romania is analyzed from a holistic perspective as a complex adaptive system by using econometric tools. The purpose of the research is to analyze the Romanian energy sector as a cybernetic system and to study the long-run and the short-run causal impact of greenhouse gas emissions (GHG) and renewable energy (RE) on real GDP per capita. The causality among GHG, foreign direct investment (FDI), RE, and real GDP is checked by means of the autoregressive distributed lag model (ARDL). The time series are extracted from Eurostat and OECD databases and cover the period 2000-2021. The results reveal that (i) the variables are cointegrated according to the ARDL bounds test; (ii) in the long run, GHG negatively impacts GDP, RE positively impacts GDP; (iii) in the short run, GHG and RE positively impact GDP; (iv) the speed of adjustment is around 32%. The study holds significance both for scholars and the policy makers from the governmental environment agencies that should decide how to effectively reduce GHG emissions, promote renewable energy adoption, and design policies to facilitate the transition to a low-carbon economy.
Local Variability of Trace Element Concentration in Barn Swallow (Hirundo rustica) Nestlings from the Po Plain (Northern Italy)
Authors: Costanzo Alessandra , Sturini Michela , Maraschi Federica , Caprioli Manuela , Romano Andrea , Vanni Simone , Parolini Marco , Profumo Antonella , Rubolini Diego , Ambrosini Roberto , Canova Luca
Published: August 2023

Birds are commonly used as bioindicators, and their feathers are considered suitable tissues for assessing the presence of contaminants, such as trace elements, in the environment. In agroecosystems, trace elements’ occurrence can be associated with both natural and anthropogenic processes, including vehicular traffic, traditional fertilizers, food feed additives for livestock, and the use of sewage sludge as fertilizer. Here, we evaluated the concentrations of twelve trace elements (Aluminium, Arsenic, Cadmium, Chromium, Copper, Iron, Mercury, Manganese, Nickel, Lead, Selenium, and Zinc) in the feathers of barn swallow (Hirundo rustica) nestlings. We then compared the concentrations of these elements between nestlings grown in areas amended or not amended with sewage sludge in 2019 and 2020 in a broad region of the Po Plain (Northern Italy). Multivariate analysis showed that the element content of the feathers significantly differed among years and areas, suggesting that the concentration of nestlings’ feathers may indicate the local level of contamination. However, univariate analyses did not show clear spatial differences, possibly due to co-occurring sources of trace elements other than sewage sludge. These results suggest that barn swallow nestlings can be a reliable sentinel for the monitoring of local variation of the environmental occurrence of trace elements.
Dynamic Analysis of Provincial Forest Carbon Storage Efficiency in China Based on DEA Malmquist Index
Authors: Liu Xuelu , Huang Jiejun , Zhou Han , Sun Jiaqi , Wang Qi , Cheng Xuejun
Published: August 2023

Global climate change caused by greenhouse gas emissions has a direct impact on the sustainable development of human society and has gained widespread concern globally. Biological carbon sequestration measures, mainly forests, are conducive to improving the ecological carbon sink capacity and play an important role in mitigating global climate change. Therefore, assessing the efficiency of forest carbon storage (FCS) is key. In view of the lack of evaluation methods for forest carbon storage efficiency (FCSE) on a large spatial scale and long time series, a method based on Data Envelopment Analysis (DEA) was proposed in this paper. The FCS was calculated using the forest storage expansion method, and the FCSE was calculated using the DEA-Malmquist index to solve the dynamic balance between regional forestry economic input and carbon storage output efficiency. The FCSE in Chinese provinces was analyzed. The results showed that from 1999 to 2018, China’s FCS increased annually, from 7558.34 million tons to 9476.73 million tons, and the spatial distribution pattern of total FCS was always different among regions. The average TFP value of FCSE was 1.089, which proves that the FCSE in China has been on the rise in the past 20 years, but the efficiency growth differed between provinces and was affected by different factors such as technical efficiency and technological progress.
Ecosystem Services Response to the Grain-for-Green Program and Urban Development in a Typical Karstland of Southwest China over a 20-Year Period
Authors: Cheng Yu , Xu Hui-Hua , Chen Si-Min , Tang Yu , Lan Zhan-Shan , Hou Guo-Long , Jiang Zhi-Yun
Published: August 2023

Southwest China is an ecologically fragile area with a high proportion of karstland and large variations in terrain, and it can be greatly affected by land use change. From 2000 to 2020, with the acceleration of urbanization in the whole country, the macro-scale Grain-for-Green Program (GFGP) has been developed in the karstland of southwest China. This has resulted in the expansion of forest and construction land with a reduction in cultivated land. The response of ecosystem services (ESs) to these changes needs to be investigated. However, there is a lack of in-depth analysis of the karstland of southwest China, and current studies mostly focus on the spatio-temporal variation in individual ESs or how the whole land use change affects ESs. Thus, our study uses an InVEST model and geographically and temporally weighted regression (GTWR) to examine the spatio-temporal variation in land use associated with four ESs, namely water conservancy (WC), soil conservancy (SC), carbon sequestration (CS), and habitat quality (HQ). We found that the GFGP area first increased and then decreased, aggregating to 4.48 × 104 km2 from 2000 to 2020. We also observed that from 2000 to 2020, ESs remained stable or gradually increased (despite fluctuations), SC was the most stable, whereas WC, CS, and HQ first decreased and then fluctuated more. This may be related to the destruction of topsoil in the early stages of the GFGP and the relatively weak ES supply capacity of the young trees. Moreover, the response of ecosystem services to the GFGP was spatially heterogeneous, suggesting a strong driving influence of the local environment, mainly caused by the distribution of karstland and terrain, differentiated urbanization levels, and the intensity of the GFGP. Specifically, the relatively significantly positive effects of WC, SC, and CS were found in western and northern cities, and so was the HQ in southeastern cities. On the contrary, the negative impacts of urbanization were found to weaken over time, suggesting the trade-off effect of the GFGP. Our findings would contribute to the development of effective forest management strategies and provide valuable insights for policymakers and stakeholders involved in ecosystem restoration and conservation efforts by exploring the impacts of the GFGP and urbanization on ESs.
Integrated Transcriptome and Biochemical Analysis Provides New Insights into the Leaf Color Change in Acer fabri
Authors: Liu Guohua , Gu Heng , Cai Hongyu , Guo Congcong , Chen Ying , Wang Lianggui , Chen Gongwei
Published: August 2023

Acer fabri is a widely distributed ornamental tree with colorful leaves and high ornamental value. Its young leaves change from red to red and green until turning fully green. To understand the mechanism of leaf color change, transcriptome sequencing and pigment content determination were performed in three stages during the leaf color change of A. fabri. In total, 53,550 genes, including 838 transcription factors (TFs), were identified by transcriptome sequencing. In addition, the results of orthogonal partial least squares-discriminant analysis (OPLS-DA) of three pigments in the three stages of leaf color development suggested that carotenoids played a major role in the process of leaf color change from red to red-green, whereas anthocyanins played an important role in the process of leaf color change from red to green. Based on weighted gene co-expression network analysis (WGCNA), Af0034384 (HSFB2A), Af0051627 (NMT1), and Af0052541 (THY-1) were selected as hub genes from characteristic modules with significant correlation between carotenoids and anthocyanins. The results of gene network regulation maps and real-time fluorescence quantitative PCR (qRT-PCR) showed that Af0010511 (NAC100) upregulated the expression of Af0034384 (HSFB2A), leading to an increase in carotenoid content and the gradual greening of leaves during the transition from red to green. However, during the transition from red to green leaves, Af0033232 (NAC83) and Af0049421 (WRKY24) downregulated the expression of Af0051627 (NMT1) and Af0052541 (THY-1), respectively, leading to a decrease in anthocyanin content and the complete greening of leaves. These results could provide new ideas for studying the molecular mechanism of leaf color change in A. fabri and other species.
Tolerance and Acclimation of the Leaves of Nine Urban Tree Species to High Temperatures
Authors: Okubo Nao , Inoue Sumihiro , Ishii H.
Published: August 2023

To prepare and manage urban greenspace for a hotter future, we must select trees that can tolerate or acclimate to high temperatures. Here, we compared tolerance and acclimation to high temperatures among nine urban tree species in Japan. Saplings were exposed to heat from different times (early and mid-summer) during the growing season in a greenhouse. With the exception of Ginko, heating in early summer did not affect whole-tree health, suggesting that most urban tree species may be able to acclimate to higher temperatures during the early growing season. However, continued exposure to higher temperatures, as well as heating from mid-summer, had negative effects on tree health, leading to wilting/browning, especially for evergreen broadleaved species whose leaves mature slowly. Cornus florida, Styrax japonicus and Morella rubra were the most vulnerable to heating, such that all heated saplings had died by the end of summer. At the leaf level, leaf maturation of the deciduous species and Morella was negatively affected by heating, whereas that of Eurya emarginata and Euonymus japonicas were not affected. These two species also showed heat tolerance, having a higher T50 (temperature where leaf quantum yield declined to 50% of maximum value due to heat stress) compared to other species, as well as heat acclimation, where T50 was higher for the heated saplings compared to the control. Our results indicate that, while some species that cannot recover from heat damage in early summer could die, others can acclimate to sustained high temperatures, as well as to late summer heat. As heatwaves are expected to become more frequent and severe due to global warming, tree species need to be screened individually to assess their ability to tolerate or acclimate to high temperature.
Formulation of A Gluten-Free Carob-Based Bakery Product: Evaluation of Glycemic Index, Antioxidant Activity, Rheological Properties, and Sensory Features
Authors: Restuccia Donatella , Esposito Luigi , Spizzirri Umile Gianfranco , Martuscelli Maria , Caputo Paolino , Rossi Cesare Oliviero , Clodoveo Maria Lisa , Pujia Roberta , Mazza Elisa , Pujia Arturo , Montalcini Tiziana , Aiello Francesca
Published: August 2023

A baked gluten-free pastry was formulated using milk kefir, rice, and different amounts of carob pulp flour, i.e., 20% (B1) and 40% (B2). In all cases, B2 showed the most remarkable antioxidant properties in terms of total phenolic, phenolic acid, and flavonoid content, as well as scavenging activity both in aqueous and organic media. This trend was observed over a 6-day storage time. Lower cohesive interactions among dough aggregate domains were recorded as the carob pulp flour amount increased. At the same time, rigidity decreased in the order B0 > B1 > B2 as confirmed by lower textural properties shown by the carob-added samples. Sensory analysis recorded overall acceptability for both B1 and B2, with sweetness, cocoa, gingerbread, and rye aroma as predominant descriptors. The glycemic index determination confirmed a better score for B2 and revealed a medium GI value (62), in comparison with high GIs recorded for B1 and B0 (115 and 100, respectively).
Real-World Implementation and Integration of an Automatic Scoring System for Workplace Safety Courses in Italian
Authors: Arici Nicola , Gerevini Alfonso , Olivato Matteo , Putelli Luca , Sigalini Luca , Serina Ivan
Published: August 2023

Artificial Intelligence and Natural Language Processing techniques can have a very significant impact on the e-learning sector, with the introduction of chatbots, automatic correctors, or scoring systems. However, integrating such technologies into the business environment in an effective way is not a trivial operation, and it not only requires realising a model with good predictive performance, but also it requires the following: (i) a proper study of the task, (ii) a data collection process, (iii) a real-world evaluation of its utility. Moreover, it is also very important to build an entire IT infrastructure that connects the AI system with the company database, with the human employees, the users, etc. In this work, we present a real-world system, based on the state-of-the-art BERT model, which implements an automatic scoring system for open-ended questions written in Italian. More specifically, these questions pertain to the workplace safety courses which every worker must attend by law, often via e-learning platforms such as the one offered by Mega Italia Media. This article describes how our system has been designed, evaluated, and finally deployed for commercial use with complete integration with the other services provided by the company.
Enhancing Network Security: A Machine Learning-Based Approach for Detecting and Mitigating Krack and Kr00k Attacks in IEEE 802.11
Authors: Salah Zaher , Abu Elsoud Esraa
Published: August 2023

The rise in internet users has brought with it the impending threat of cybercrime as the Internet of Things (IoT) increases and the introduction of 5G technologies continues to transform our digital world. It is now essential to protect communication networks from illegal intrusions to guarantee data integrity and user privacy. In this situation, machine learning techniques used in data mining have proven to be effective tools for constructing intrusion detection systems (IDS) and improving their precision. We use the well-known AWID3 dataset, a comprehensive collection of wireless network traffic, to investigate the effectiveness of machine learning in enhancing network security. Our work primarily concentrates on Krack and Kr00k attacks, which target the most recent and dangerous flaws in IEEE 802.11 protocols. Through diligent implementation, we were able to successfully identify these threats using an IDS model that is based on machine learning. Notably, the resilience of our method was demonstrated by our ensemble classifier’s astounding 99% success rate in detecting the Krack attack. The effectiveness of our suggested remedy was further demonstrated by the high accuracy rate of 96.7% displayed by our neural network-based model in recognizing instances of the Kr00k attack. Our research shows the potential for considerably boosting network security in the face of new threats by leveraging the capabilities of machine learning and a diversified dataset. Our findings open the door for stronger, more proactive security measures to protect IEEE. 802.11 networks’ integrity, resulting in a safer online environment for all users.
Successful Applications of NIR Spectroscopy and NIR Imaging in the Food Processing Chain
Authors: Squeo Giacomo , Amigo José Manuel
Published: August 2023

Forty years ago, Near InfraRed (NIR) was considered a sleeping technique among the spectroscopic ones [...]
Combined Computer-Aided Predictors to Improve the Thermostability of Nattokinase
Authors: Li Yuan , Chen Liangqi , Tang Xiyu , Zhu Wenhui , Ma Aixia , Shi Changyu , Li Jinyao
Published: August 2023

Food-derived nattokinase has strong thrombolytic activity and few side effects. In the field of medicine, nattokinase has been developed as an adjuvant drug for the treatment of thrombosis, and nattokinase-rich beverages and health foods have also shown great potential in the field of food development. At present, the poor thermostability of nattokinase limits its industrial production and application. In this study, we used several thermostability-prediction algorithms to predict nattokinase from Bacillus mojavensis LY-06 (AprY), and screened two variants S33T and T174V with increased thermostability and fibrinolytic activity. The t1/2 of S33T and T174V were 8.87-fold and 2.51-fold those of the wild type AprY, respectively, and their enzyme activities were also increased (1.17-fold and 1.28-fold, respectively). Although the thermostability of N218L was increased by 2.7 times, the fibrinolytic activity of N218L was only 73.3% of that of wild type AprY. The multiple-point mutation results showed that S33T-N218L and S33T-T174V-N218L variants lost their activity, and the T174V-N218L variant did not show any significant change in catalytic performance, while S33T-T174V increased its thermostability and activity by 21.3% and 24.8%, respectively. Although the S33T-T174V variant did not show the additive effect of thermostability, it combined the excellent transient thermostability of S33T with the better thrombolytic activity of T174V. Bioinformatics analysis showed that the overall structure of S33T and T174V variants tended to be stable, while the structure of S33T-T174V variant was more flexible. Local structure analysis showed that the increased rigidity of the active center region (positions 64–75) and the key loop region (positions 129–130, 155–163, 187–192, 237–241, and 268–270) determined the increased thermostability of all variants. In addition, the enhanced flexibility of S33T-T174V variant in the Ca1 binding region (positions 1–4, 75–82) and the peripheral region of the catalytic pocket (positions 210–216) may account for the inability to superpose its thermostability. We explored the effective strategy to enhance the thermostability of nattokinase, and the resulting variants have potential industrial production and application.
The Nutritional and Functional Properties of Protein Isolates from Defatted Chia Flour Using Different Extraction pH
Authors: Khushairay Etty Syarmila Ibrahim , Ghani Ma’aruf Abd , Babji Abdul Salam , Yusop Salma Mohamad
Published: August 2023

This study aims to determine the effects of various alkaline pHs on the nutritional and functional properties of protein isolated from defatted chia flour (DCF). The DCF isolated using alkali extraction method at pH 8.5, 10.0, and 12.0 were coded as CPI-8.5, CPI-10.0, and CPI-12.0, respectively. The highest extraction yield and protein recovery yield was demonstrated by CPI-12.0 (19.10 and 59.63%, respectively), with a total protein content of 74.53%, and glutelin showed the highest portion (79.95%). The CPI-12.0 also demonstrated the most elevated essential (36.87%), hydrophobic (33.81%), and aromatic (15.54%) amino acid content among other samples. The DCF exhibited the highest water (23.90 gg−1) and oil (8.23 gg−1) absorption capacity, whereas the CPI-8.5 showed the highest protein solubility (72.31%) at pH 11. DCF demonstrated the highest emulsifying capacity at pH 11 (82.13%), but the highest stability was shown at pH 5 (82.05%). Furthermore, CPI-12.0 at pH 11 shows the highest foaming capacity (83.16%) and stability (83.10%). Despite that, the CPI-10.0 manifested the highest antioxidant capacity (DPPH: 42.48%; ABTS: 66.23%; FRAP: 0.19), as well as ACE-I (35.67%). Overall, the extraction pH had significant effects in producing chia protein isolates (CPI) with improved nutritional and functional qualities.
Tomato-Thaumatin-like Protein Genes Solyc08g080660 and Solyc08g080670 Confer Resistance to Five Soil-Borne Diseases by Enhancing β-1,3-Glucanase Activity
Authors: Li Xinyun , Xu Bin , Xu Junqiang , Li Zuosen , Jiang Caiqian , Zhou Ying , Yang Zhengan , Deng Minghua , Lv Junheng , Zhao Kai
Published: August 2023

Although thaumatin-like proteins (TLPs) are involved in resistance to a variety of fungal diseases, whether the TLP5 and TLP6 genes in tomato plants (Solanum lycopersicum) confer resistance to the pathogenesis of soil-borne diseases has not been demonstrated. In this study, five soil-borne diseases (fungal pathogens: Fusarium solani, Fusarium oxysporum, and Verticillium dahliae; bacterial pathogens: Clavibacter michiganense subsp. michiganense and Ralstonia solanacearum) were used to infect susceptible “No. 5” and disease-resistant “S-55” tomato cultivars. We found that SlTLP5 and SlTLP6 transcript levels were higher in susceptible cultivars treated with the three fungal pathogens than in those treated with the two bacterial pathogens and that transcript levels varied depending on the pathogen. Moreover, the SlTLP5 and SlTLP6 transcript levels were much higher in disease-resistant cultivars than in disease-susceptible cultivars, and the SlTLP5 and SlTLP6 transcript levels were higher in cultivars treated with the same fungal pathogen than in those treated with bacterial pathogens. SlTLP6 transcript levels were higher than SlTLP5. SlTLP5 and SlTLP6 overexpression and gene-edited transgenic mutants were generated in both susceptible and resistant cultivars. Overexpression and knockout increased and decreased resistance to the five diseases, respectively. Transgenic plants overexpressing SlTLP5 and SlTLP6 inhibited the activities of peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) after inoculation with fungal pathogens, and the activities of POD, SOD, and APX were similar to those of fungi after infection with bacterial pathogens. The activities of CAT were increased, and the activity of β-1,3-glucanase was increased in both the fungal and bacterial treatments. Overexpressed plants were more resistant than the control plants. After SlTLP5 and SlTLP6 knockout plants were inoculated, POD, SOD, and APX had no significant changes, but CAT activity increased and decreased significantly after the fungal and bacterial treatments, contrary to overexpression. The activity of β-1,3-glucanase decreased in the treatment of the five pathogens, and the knocked-out plants were more susceptible to disease than the control. In summary, this study contributes to the further understanding of TLP disease resistance mechanisms in tomato plants.
Genome-Wide Single-Nucleotide Polymorphism-Based Genomic Diversity and Runs of Homozygosity for Selection Signatures in Equine Breeds
Authors: Bhardwaj Anuradha , Tandon Gitanjali , Pal Yash , Sharma Nitesh Kumar , Nayan Varij , Soni Sonali , Iquebal Mir Asif , Jaiswal Sarika , Legha Ram Avatar , Talluri Thirumala Rao , Bhattacharya Tarun Kumar , Kumar Dinesh , Rai Anil , Tripathi B. N.
Published: August 2023

The horse, one of the most domesticated animals, has been used for several purposes, like transportation, hunting, in sport, or for agriculture-related works. Kathiawari, Marwari, Manipuri, Zanskari, Bhutia, Spiti, and Thoroughbred are the main breeds of horses, particularly due to their agroclimatic adaptation and role in any kind of strong physical activity, and these characteristics are majorly governed by genetic factors. The genetic diversity and phylogenetic relationship of these Indian equine breeds using microsatellite markers have been reported, but further studies exploring the SNP diversity and runs of homozygosity revealing the selection signature of breeds are still warranted. In our study, the identification of genes that play a vital role in muscle development is performed through SNP detection via the whole-genome sequencing approach. A total of 96 samples, categorized under seven breeds, and 620,721 SNPs were considered to ascertain the ROH patterns amongst all the seven breeds. Over 5444 ROH islands were mined, and the maximum number of ROHs was found to be present in Zanskari, while Thoroughbred was confined to the lowest number of ROHs. Gene enrichment of these ROH islands produced 6757 functional genes, with AGPAT1, CLEC4, and CFAP20 as important gene families. However, QTL annotation revealed that the maximum QTLs were associated with Wither’s height trait ontology that falls under the growth trait in all seven breeds. An Equine SNP marker database (EqSNPDb) was developed to catalogue ROHs for all these equine breeds for the flexible and easy chromosome-wise retrieval of ROH along with the genotype details of all the SNPs. Such a study can reveal breed divergence in different climatic and ecological conditions.
A Model to Prevent Substance Use/Abuse by Student Nurses at Limpopo College of Nursing, South Africa
Authors: Netshiswinzhe Matodzi Doris , Ramathuba Dorah Ursula , Lebese Tsakani Rachel , Makhado Lufuno
Published: August 2023

Background: Substance use/abuse is a global challenge that has detrimental effects on nations’ health, wealth, and security. Substance users in Africa make up roughly 17% to 21% of global illicit drug users in Africa and cannabis abuse (63%) (UNODC, 2010). Purpose: We aimed to develop, describe, and evaluate a model that could be used as a framework of reference to prevent substance use/abuse by student nurses at Limpopo College of Nursing, South Africa. Method: A mixed-method study approach was used following a qualitative exploratory, descriptive, and contextual design to explore factors contributing to substance use/abuse by student nurses and quantitatively examine the impact of substance use/abuse on students’ academic achievements in Limpopo Province. Findings: The results of the first empirical phase reveal two themes and seven subthemes from the semi-structured interviews with lecturers, students, and support staff. The literature supported the results. In phase 2, we analyzed the concept of the “prevention” of substance use/abuse by student nurses following the process of a concept analysis by Walker and Avant (2016). The results were conceptualized within the six elements of practice theory: context, agent, recipient, dynamic, process and procedure, and outcome. Conclusions: The relational statements provided the basis for the model description. A reliable method was used to describe and evaluate the model.
Effectiveness of Physical Exercise on Pain, Disability, Job Stress, and Quality of Life in Office Workers with Chronic Non-Specific Neck Pain: A Randomized Controlled Trial
Authors: Alshehre Yousef M. , Pakkir Mohamed Shahul Hameed , Nambi Gopal , Almutairi Sattam M. , Alharazi Ahmed A.
Published: August 2023

Neck pain is a widespread medical condition among office workers worldwide. This study aimed to compare physical exercises, including basic body awareness, neck-specific training exercises and ergonomic modifications, and ergonomic modifications alone in the management of chronic non-specific neck pain (NSNP) among office workers. Sixty participants were randomly allocated to an experimental group (physical exercises and ergonomic modifications) or a control group (ergonomic modifications) and received the intervention two times a week for eight weeks. The Numerical Pain Rating Scale, Neck Disability Index, Health and Safety Stress Tool, and Short Form Health Survey-36 were used to measure pain, disability, job stress, and quality of life at baseline, and at weeks 4 and 8 of the study period. A repeated measure ANOVA was used to determine the within-group significant differences and an independent t-test was utilized to compare group differences. The baseline data of the experimental and control groups showed no significant difference (p ≥ 0.05). The 2 × 3 mixed models ANOVA showed a significant difference in pain intensity (p = 0.001, ηp2 = 0.042), functional disability (p = 0.001, ηp2 = 0.052), work-related stress (p = 0.001, ηp2 = 0.036), and QoL (p = 0.012, ηp2 = 0.025). Four weeks post-intervention, the experimental group showed significant changes in primary (pain intensity and disability) (1.9; 95% confidence interval 1.65–2.14) and secondary (quality of life and work-related stress) outcomes (p < 0.001). The same gradual improvement in these variables was observed in the 8-week follow-up (p < 0.001). There was a significant improvement in clinical outcomes following the application of physical exercises with ergonomic modifications for chronic NSNP among office workers. This is significant for office workers because it suggests the importance of incorporating physical exercises into their daily routine and making ergonomic changes to their workspaces.
Pediatric Neurology Workforce in Saudi Arabia: A 5-Year Update
Authors: Bamaga Ahmed K. , Alyazidi Anas S. , Almubarak Albatool , Almohammal Mohammad N. , Alharthi Ayidh S. , Alsehemi Matar A.
Published: August 2023

Background: The medical workforce plays a pivotal role in advancing human health, particularly within the healthcare system of Saudi Arabia. While government-employed healthcare providers form the central structure of the system and offer free healthcare services, the private healthcare sector is also witnessing significant growth. In parallel, the field of child neurology has experienced notable transformations in recent years, with continued expansion. This expansion brings forth a range of challenges for both current and future pediatric neurologists, necessitating careful consideration and proactive measures to address them. Aim of the study: To investigate and analyze the current characteristics of the workforce, with a specific focus on their employment status and related data. Methods: This is a cross-sectional analysis, using a survey to assess the distribution of pediatric neurologists in Saudi Arabia (SA). The final analytical sample included 82 subjects, working in 13 regions in SA. A descriptive analysis was used to address the study question. Results: The survey received responses from a total of 82 pediatric neurologists in Saudi Arabia (response rate 55%), with 38 (46%) being men and 44 (54%) being women. The mean age was 33 ± 1.225 years. The majority of participants practiced in major cities such as Riyadh and Jeddah. Nearly 50% of pediatric neurologists experienced some form of delay in obtaining their first job, ranging from 1 to 36 months. Conclusion: The landscape of the pediatric neurology workforce is currently witnessing noteworthy demographic shifts. With the majority of practitioners concentrated in major cities, there is an ongoing demand for qualified professionals in peripheral areas. This study describes the real-life challenges faced by pediatric neurologists, particularly the delay in securing employment after graduation, and underscores the critical importance of addressing these persistent issues along the journey of pediatric neurology.
National Assessment of Opportunities for Improvement in Preventable Trauma Deaths: A Mixed-Methods Study
Authors: Kwon Junsik , Lee Myeonggyun , Jung Kyoungwon
Published: August 2023

Trauma is a significant public health issue worldwide, particularly affecting economically active age groups. Quality management of trauma care at the national level is crucial to improve outcomes of major trauma. In Korea, a biennial nationwide survey on preventable trauma death rate is conducted. Based on the survey results, we analyzed opportunities for improving the trauma treatment process. Expert panels reviewed records of 8282 and 8482 trauma-related deaths in 2017 and 2019, respectively, identifying 258 and 160 cases in each year as preventable deaths. Opportunities for improvement were categorized into prehospital, interhospital, and hospital stages. Hemorrhage was the primary cause of death, followed by sepsis/multiorgan failure and central nervous system injury. Delayed hemostatic procedures and transfusions were common areas for improvement in hospital stage. Interhospital transfers experienced significant delays in arrival time. This study emphasizes the need to enhance trauma care by refining treatment techniques, centralizing patients in specialized facilities, and implementing comprehensive reviews and performance improvements throughout the patient transfer system. The findings offer valuable insights for addressing trauma care improvement from both clinical and systemic perspectives.
Effects of Physical Activity Program on Body Composition, Physical Performance, and Neuromuscular Strategies during Walking in Older Adults with Sarcopenic Obesity: Randomized Controlled Trial
Authors: Ferhi Hamza , Gaied Chortane Sabri , Durand Sylvain , Beaune Bruno , Boyas Sébastien , Maktouf Wael
Published: August 2023

The potential impact of a specific physical activity program on biomechanical gait parameters and neuromuscular strategies around the ankle joint in older adults with sarcopenic obesity (SO) remains largely unexplored. The objective of this study was to investigate the effectiveness of a 24-week posture, strengthening, and motricity (PSM) program on improving neuromuscular strategies and biomechanical gait parameters in older adults with SO. 40 participants were randomly assigned to either the trained group (TG) and the control group (CG). Only the TG received the PSM program. Standardized evaluations were performed before and after the intervention, including walking tests on an instrumented gait analysis treadmill to evaluate biomechanical gait parameters and EMG activity of ankle muscles. After the PSM program, TG exhibited an increase in comfortable walking speed (+80%, p < 0.001) and step length (+38%, p < 0.05). Moreover, TG demonstrated a reduction in CoP velocity (−26%, p < 0.01). These gait modifications were associated with decreased muscle activity during the different gait phases (p < 0.05). The PSM program effectively improved gait and neuromuscular capacities in older adults with SO. Notably, these results shed light on the remarkable trainability of neuromuscular capacities in older adults with SO, despite the adverse effects of aging and obesity.
Control of Nematodes in Organic Horticulture Exploiting the Multifunctional Capacity of Microorganisms
Authors: Furmanczyk Ewa M. , Malusà Eligio
Published: August 2023

Organic production is expected to play a major role in reducing the impact of agricultural practices on the environment. Soil is considered a major component of the organic production process, and organic practices aim at increasing its health and fertility. However, the control of soil-borne pests, particularly plant-parasitic nematodes, can be difficult in organic horticultural crops due to the rules allowed in this farming system. Applying a holistic approach that fosters and exploits the activity of the soil microbiome to control plant-parasitic nematodes has been at the basis of the analysis of the available scientific knowledge carried out for this review article. This review thus focuses on the multifunctional capacity of microorganisms, including that of bacteria and fungi not normally considered biocontrol agents, and the need to also better understand their relations with the plant and other environmental and agronomic factors. The implementation of the “multi-biotics” concept, applying prebiotics, probiotics and postbiotics, which supports an integrated agroecological strategy for the protection of organic horticultural crops, is proposed as an efficient practice that should be further studied to be adapted under different crops and pedo-climatic conditions.
A Study on the Causes of Apomixis in Malus shizongensis
Authors: Feng Yuchen , Ning Ruiyuan , Wang Zidun , He Ying , Hu Yu , Sun Lulong , Liu Zhenzhong
Published: August 2023

Apomixis is a unique reproductive process that produces fertile offspring without the combination of sperm and egg cells. This process perfectly reproduces maternal DNA, making it possible to fix heterosis during reproduction. Malus shizongensis is a newly discovered species that is closely related to Malus hupehensis Rehd. After de-male bagging, it was found that the fruit set rate reached 78.7%. Preliminary analysis indicated that M. shizongensis have apomictic reproductive characteristics. In this work, we employed paraffin sectioning and electron scanning microscopy to explore apomixis in M. shizongensis during the development of male–female gametes and embryo sacs. Stigma fluorescence assays showed that pollen germination was normal, but less pollen entered the ovaries. Additionally, analysis of anthers indicated the presence of dysplasia and paraffin sectioning revealed that the pollen mother cells were aborted due to abnormal disintegration of the tapetum layer. Taken together, our results indicate that the primary causes of apomixis in M. shizongensis are anther dysplasia and male gamete development failure, resulting in reduced pollen tube entry into ovaries and reduced reproduction of female gametes. In conclusion, this study provide a theoretical basis and technical supports for apple stock breeding and apple industry development.