bluuebunny/crossref_metadata_2025 huggingface dataset:
The Physiological and Psychological Benefits of Dance and its Effects on Children and Adolescents: A Systematic Review
Authors: Tao Dan , Gao Yang , Cole Alistair , Baker Julien S. , Gu Yaodong , Supriya Rashmi , Tong Tomas K. , Hu Qiuli , Awan-Scully Roger
Published: June 2022

Background: The aim of this review was to examine the physiological and psychological benefits of dance and its effects on children and adolescents. We consider the therapeutic benefits of dance and outline the potential of dance as an alternative therapy for certain pathologies and medical disorders. Secondly, we summarize the types of dances used in physical interventions, and comment on the methodologies used. Finally, we consider the use of dance as a different exercise modality that may have benefits for increased physical activity generally, and for increased physical education provision in schools.

Methods: A structured search strategy was conducted using the databases of PubMed, MEDLINE, Web of science, PsycARTICLES, and Social Science database. This review used the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines for systematic reviews. Studies that were published in the past 20 years were considered for inclusion. All written publications were searched for in English, and all articles included in this review were peer reviewed full papers.

Conclusion: The key findings from this review indicate that dance is a feasible alternative to traditional physical activity. The findings also indicate that dance provides physiological and psychological benefits to healthy and medically compromised populations. Implementation of dance programs in schools and society generally needs serious consideration by policy makers. We hope that the results of this review stimulate debate and provide the necessary evidence to profile dance as a viable alternative medium of physical activity. Comprehensive and integrated changes will be needed including economical and legislative support from politicians and associated governmental agencies. The findings reported here are important and have implications for health policy change, reconfiguration, and implementation.
Nitrogen Addition Affects Ecosystem Carbon Exchange by Regulating Plant Community Assembly and Altering Soil Properties in an Alpine Meadow on the Qinghai–Tibetan Plateau
Authors: Han Ling , Ganjurjav Hasbagan , Hu Guozheng , Wu Jianshuang , Yan Yulong , Danjiu Luobu , He Shicheng , Xie Wendong , Yan Jun , Gao Qingzhu
Published: June 2022

Nitrogen (N) deposition can affect the global ecosystem carbon balance. However, how plant community assembly regulates the ecosystem carbon exchange in response to the N deposition remains largely unclear, especially in alpine meadows. In this study, we conducted a manipulative experiment to examine the impacts of N (ammonium nitrate) addition on ecosystem carbon dioxide (CO2) exchange by changing the plant community assembly and soil properties at an alpine meadow site on the Qinghai–Tibetan Plateau from 2014 to 2018. The N-addition treatments were N0, N7, N20, and N40 (0, 7, 20, and 40 kg N ha–1year–1) during the plant growing season. The net ecosystem CO2 exchange (NEE), gross ecosystem productivity (GEP), and ecosystem respiration (ER) were measured by a static chamber method. Our results showed that the growing-season NEE, ER and GEP increased gradually over time with increasing N-addition rates. On average, the NEE increased significantly by 55.6 and 65.2% in N20 and N40, respectively (p < 0.05). Nitrogen addition also increased forage grass biomass (GB, including sedge and Gramineae) by 74.3 and 122.9% and forb biomass (FB) by 73.4 and 51.4% in N20 and N40, respectively (p < 0.05). There were positive correlations between CO2 fluxes (NEE and GEP) and GB (p < 0.01), and the ER was positively correlated with functional group biomass (GB and FB) and soil available N content (NO3––N and NH4+–N) (p < 0.01). The N-induced shift in the plant community assembly was primarily responsible for the increase in NEE. The increase in GB mainly contributed to the N stimulation of NEE, and FB and the soil available N content had positive effects on ER in response to N addition. Our results highlight that the plant community assembly is critical in regulating the ecosystem carbon exchange response to the N deposition in alpine ecosystems.
Stubby or Slender? Ear Architecture Is Related to Drought Resistance in Maize
Authors: Chen Xian-Min , Li Feng-Yuan , Dong Shuai , Liu Xin-Fang , Li Bin-Bin , Xiao Zu-Dong , Deng Tao , Wang Yan-Bo , Shen Si , Zhou Shun-Li
Published: June 2022

Ear architecture is determined by two stable heritable traits, kernel row number (KRN) and kernel number per row (KNPR), but its relationship with drought resistance is still vague. To this end, we obtained 16 and 11 hybrids with slender (less KRN but more KNPR) and stubby (more KRN but less KNPR) ears by intentionally crossbreeding, respectively. These hybrids were exposed to a seven-day water deficit (WD) since silk emergence coupled with synchronous (SP) and continuous pollination (CP) to alter the pollination time gaps on ears. The results showed that the emerged silks in CP were 9.1 and 9.0% less than in the SP treatment in the stubby and slender ears, respectively, suggesting the suppression of asynchronous pollination on silk emergence. The stubby ears performed higher silking rate and yield compared with the slender ears with or without drought stress. To eliminate the inherent difference in sink capacities, we selected four hybrids for each ear type with similar silk and kernel numbers for further analyses. Interestingly, the stubby ears were less affected in silking rate and thus performed higher yield under drought compared with the slender ears. The finding suggests that ear architecture matters in the determination of drought resistance that deserves more attention in breeding.
Forestry Digital Twin With Machine Learning in Landsat 7 Data
Authors: Jiang Xuetao , Jiang Meiyu , Gou YuChun , Li Qian , Zhou Qingguo
Published: June 2022

Forest succession analysis can predict forest change trends in the study area, which provides an important basis for other studies. Remote sensing is a recognized and effective tool in forestry succession analysis. Many forest modeling studies use statistic values, but only a few uses remote sensing images. In this study, we propose a machine learning-based digital twin approach for forestry. A data processing algorithm was designed to process Landsat 7 remote sensing data as model's input. An LSTM-based model was constructed to fit historical image data of the study area. The experimental results show that this study's digital twin method can effectively forecast the study area's future image.
How to Realize Business Model Innovation for New Ventures? Psychological Capital and Social Capital Perspective
Authors: Zhou Jian , Liu Yubo , Yang Peng , Cao Qinqin
Published: June 2022

Business model innovation has become a necessary means for enterprises to break through path constraints, achieve sustainable development, and obtain sustainable competitiveness, which has been paid more and more attention by entrepreneurs and scholars. Based on the resource conversation theory and signal theory, this study constructs a research model with psychological capital (PC) and social capital (SC) as independent variables and business model innovation as dependent variables along the logical path of “resource acquisition-resource utilization.” By dividing business model innovation into pioneering business model innovation and perfect business model innovation, we use fuzzy-set qualitative comparative analysis (fsQCA) to explore the impact of dual capital on business model innovation of new ventures. This study takes entrepreneurs from the eastern part of China's seven entrepreneurship active provinces as investigation objects, based on the analysis of the collected 242 valid questionnaire results, emphasizing that any single antecedent can not be a sufficient and necessary condition for pioneering and perfect business model innovation. In this case, we carried out research with a certain antecedent variable as the core and supplemented with other antecedent variables to form seven different configurations. The results showed that the combination of the antecedent variables could effectively achieve the pioneering and perfect business model innovation. The theoretical contributions of this study are as follows: (1) it enriches the research on the antecedents of business model innovation in new ventures; (2) it expands the application scenarios of resource conversation theory and signal theory; and (3) it is emphasized that the innovation of business model of new ventures is the result of the interaction and value-added linkage of various internal and external resources.
Iconicity as Multimodal, Polysemiotic, and Plurifunctional
Authors: Hodge Gabrielle , Ferrara Lindsay
Published: June 2022

Investigations of iconicity in language, whereby interactants coordinate meaningful bodily actions to create resemblances, are prevalent across the human communication sciences. However, when it comes to analysing and comparing iconicity across different interactions (e.g., deaf, deafblind, hearing) and modes of communication (e.g., manual signs, speech, writing), it is not always clear we are looking at the same thing. For example, tokens of spoken ideophones and manual depicting actions may both be analysed as iconic forms. Yet spoken ideophones may signal depictive and descriptive qualities via speech, while manual actions may signal depictive, descriptive, and indexical qualities via the shape, movement, and placement of the hands in space. Furthermore, each may co-occur with other semiotics articulated with the face, hands, and body within composite utterances. The paradigm of iconicity as a single property is too broad and coarse for comparative semiotics, as important details necessary for understanding the range of human communicative potentialities may be masked. Here, we draw on semiotic approaches to language and communication, including the model of language as signalled via describing, indicating and/or depicting and the notion of non-referential indexicality, to illustrate the multidimensionality of iconicity in co-present interactions. This builds on our earlier proposal for analysing how different methods of semiotic signalling are combined in multimodal language use. We discuss some implications for the language and communication sciences and explain how this approach may inform a theory of biosemiotics.
Sport Participation in Early and Middle Adolescence: The Interplay Between Self-Perception and Psychobiosocial Experiences in Predicting Burnout Symptoms
Authors: Morano Milena , Robazza Claudio , Ruiz Montse C. , Bortoli Laura
Published: June 2022

Adolescence is characterized by pubertal physical changes, cognitive development, and modified social expectations. Adolescent athletes often enter a more challenging stage of athletic development associated with increased specialization, and become vulnerable to feelings of burnout. It is therefore important to consider intrapersonal psychological factors that can improve sport participation experiences and prevent burnout. Accordingly, the aim of the current study was to examine the interplay between self-perceptions and emotion-related (i.e., psychobiosocial) experiences (e.g., feeling confident, focused, determined, physically charged, and skillful) in predicting burnout symptoms in adolescents. A sample of 12–14-year-olds (n= 338, 176 girls and 162 boys;Mage = 13.42,SD= 1.12) and 15–17-year-olds (n= 302, 142 girls and 160 boys;Mage = 15.78,SD= 1.17), participating in individual or team sports, were involved in a cross-sectional study to assess positive and negative self-perceptions, functional and dysfunctional psychobiosocial experiences, and burnout symptoms (i.e., emotional and physical exhaustion, reduced sense of accomplishment, sport devaluation). Path analysis results suggest that higher scores on global physical self-perception, self-esteem, and sport competence were associated with lower burnout symptoms, while higher scores on social physique anxiety were associated with higher scores on sport devaluation. Moreover, self-esteem and sport competence were shown to have significant indirect effects on burnout dimensions via functional psychobiosocial experiences. Differences by gender (p< 0.001) and by age category (p< 0.001) in the variable scores were also found. Compared to girls, boys reported higher scores on competence, functional psychobiosocial experiences, global physical self-perception, self-esteem, emotional and physical exhaustion, and lower scores on social physique anxiety. Compared to 12–14-year-olds, 15–17-year-olds reported lower scores on global physical self-perception and self-esteem, and higher scores on social physique anxiety, reduced sense of accomplishment, and sport devaluation. This study adds to the literature on burnout by considering the role of intrapersonal factors (i.e., global physical self-perception, self-esteem, sport competence, and social anxiety) in predicting burnout symptoms in adolescent athletes, and the mediating effects of psychobiosocial experiences. From an applied perspective, sport coaches should implement strategies to foster positive self-perceptions, promote pleasant psychobiosocial experiences, and prevent burnout.
Implicit Bias Reflects the Company That Words Keep
Authors: Hauser David J. , Schwarz Norbert
Published: June 2022

In everyday language, concepts appear alongside (i.e., collocate with) related concepts. Societal biases often emerge in these collocations; e.g., female (vs. male) names collocate with art- (vs. science-) related concepts, and African American (vs. White American) names collocate with negative (vs. positive) concepts. It is unknown whether such collocations merely reflect societal biases or contribute to them. Concepts that are themselves neutral in valence but nevertheless collocate with valenced concepts provide a unique opportunity to address this question. For example, when asked, most people evaluate the concept “cause” as neutral, but “cause” is frequently followed by negative concepts (e.g., death, pain, and trouble). We use such semantically prosodic concepts to test the influence of collocation on the emergence of implicit bias: do neutral concepts that frequently collocate with valenced concepts have corresponding implicit bias? In evaluative priming tasks, participants evaluated positive/negative nouns (Study 1) or pictures (Study 2) after seeing verb primes that were (a) strongly valenced (e.g., hate and comfort), (b) neutral in valence but collocated with valenced concepts in corpora (e.g., ease and gain), or (c) neutral in valence and not collocated with valenced concepts in corpora (e.g., reply and describe). Throughout, neutral primes with positive (negative) collocates facilitated the evaluation of positive (negative) targets much like strongly valenced primes, whereas neutral primes without valenced collocates did not. That neutral concepts with valenced collocates parallel the influence of valenced concepts suggests that their collocations in natural language may be sufficient for fostering implicit bias. Societal implications of the causal embedding hypothesis are discussed.
On Students’ Willingness to Use Online Learning: A Privacy Calculus Theory Approach
Authors: Jiang Xinyu , Goh Tiong-Thye , Liu Mengjun
Published: June 2022

Online learning platforms frequently collect and store learners’ data to personalize content and improve learning analytics, but this also increases the likelihood of privacy breaches which may reduce learners’ willingness to use online learning. This study aims to examine how perceptions of benefits, privacy, risk, and trust affect students’ willingness to use online learning. We used the Privacy Calculus Theory as a theoretical framework for this study. To test the model, we surveyed 203 undergraduate students who used online learning. The results of the AMOS analysis revealed that students’ risk perception has a significant negative effect on their willingness to use online learning, while their benefit perception and trust perception have positive effects. Furthermore, the study found that improved trust can reduce perceived risk and improve willingness to use online learning. Interestingly, privacy perception is not a significant predictor of students’ willingness to use online learning, although it is a high concern factor. Discussion and conclusion are discussed at the end.
Do Local Religious Beliefs Affect Firms’ Earnings Management Practice? Evidence From the United States
Authors: Geng Hongying , Hua Min , Sun Li , Yan Chao
Published: June 2022

This paper investigates whether local religious beliefs have a significant impact on the practice of earnings management. We extend the existing literature on the role of firm characteristics in mitigating earnings management by showing that local religious beliefs significantly impact the practice of earnings management. Specifically, exploring firms located in the U.S. counties that vary from 2000 through 2010, we document the negative relationship between religiosity and earnings management using multivariate regression analysis. Our results show that firms in counties with strong religious social norms are less likely to engage in earnings management. Furthermore, we attempt to mitigate endogeneity concerns by employing a modified Difference-Differences model and Propensity score matching methods. We find that the negative effects of religion on earnings management still hold. Overall, these findings emphasize the empirical relevance of the association between the local social norms and earnings manipulations.
Auditory Target Detection Enhances Visual Processing and Hippocampal Functional Connectivity
Authors: Moyal Roy , Turker Hamid B. , Luh Wen-Ming , Swallow Khena M.
Published: June 2022

Though dividing one’s attention between two input streams typically impairs performance, detecting a behaviorally relevant stimulus can sometimes enhance the encoding of unrelated information presented at the same time. Previous research has shown that selection of this kind boosts visual cortical activity and memory for concurrent items. An important unanswered question is whether such effects are reflected in processing quality and functional connectivity in visual regions and in the hippocampus. In this fMRI study, participants were asked to memorize a stream of naturalistic images and press a button only when they heard a predefined target tone (400 or 1,200 Hz, counterbalanced). Images could be presented with a target tone, with a distractor tone, or without a tone. Auditory target detection increased activity throughout the ventral visual cortex but lowered it in the hippocampus. Enhancements in functional connectivity between the ventral visual cortex and the hippocampus were also observed following auditory targets. Multi-voxel pattern classification of image category was more accurate on target tone trials than on distractor and no tone trials in the fusiform gyrus and parahippocampal gyrus. This effect was stronger in visual cortical clusters whose activity was more correlated with the hippocampus on target tone than on distractor tone trials. In agreement with accounts suggesting that subcortical noradrenergic influences play a role in the attentional boost effect, auditory target detection also caused an increase in locus coeruleus activity and phasic pupil responses. These findings outline a network of cortical and subcortical regions that are involved in the selection and processing of information presented at behaviorally relevant moments.
Sexual Selection and the Treatment of Predecessors’ Progeny by Replacement Mates
Authors: Daly Martin , Perry Gretchen
Published: June 2022

Darwin’s theory of sexual selection provides a useful framework for understanding the behavior of stepparents. A non-human animal whose new mate has dependent young may kill, ignore, or adopt the predecessor’s progeny. The third option has been interpreted as courtship (“mating effort”), and whether selection favors such investment over killing or ignoring the young apparently depends on aspects of the species-typical ecology and demography. The tripartite categorization of responses is a simplification, however, There is variability both within and between species along a continuum from rejection to “full adoption.” The average stepparent invests less than the average birth parent, but more than nothing. Human stepparents have often been found to kill young children at higher rates than birth parents, but stepparental infanticide cannot plausibly be interpreted as a human adaptation, both because it is extremely rare and because it is almost certainly more likely to reduce the killer’s fitness than to raise it. How sexual selection theory remains relevant to human stepparenting is by suggesting testable hypotheses about predictors of the variability in stepparental investment.
Determinants of Anxiety in the General Latvian Population During the COVID-19 State of Emergency
Authors: Vrublevska Jelena , Perepjolkina Viktorija , Martinsone Kristine , Kolesnikova Jelena , Krone Ilona , Smirnova Daria , Fountoulakis Konstantinos N. , Rancans Elmars
Published: June 2022

Background

The COVID-19 pandemic and its restrictive public health measures have seriously affected mental health of society. Social, psychological, and health-related factors have been linked to anxiety in the general population.

Aim

We investigate the association of various sociopsychological and health-related determinants of anxiety and identify the predicting factors for anxiety in the general population during the COVID-19 state of emergency from in Latvia.

Methods

We conducted an online survey using a randomized stratified sample of the general adult population in July 2020 for 3 weeks. Anxiety symptoms were measured using the State-Trait Anxiety Inventory (STAI-S). Sociodemographic, health-related, sociopsychological characteristics and suicidality were identified using the structured questionnaire. The statistical analysis included Pearson's chi-square test, post hoc analysis, and binomial logistic regression.

Results

The weighted study sample included 2,608 participants. The mean STAY-S score of the total sample was 22.88 ± 12.25. In the total sample, 15.2% (n = 398) of participants were classified as having anxiety. The odds ratio (OR) of having anxiety was higher in females (OR = 2.44; 95% CI 1.75–3.33) and people who had experienced mental health problems in the past (OR = 1.45; 95% CI 1.03–2.04), had suicide attempt in the past (OR = 1.68; 95% CI 1.08–2.59), were worried about their health status due to COVID-19 (OR = 1.64; 95% CI 1.36–1.16), were worried about stigmatization from others if infected with COVID-19 (OR = 1.18; 95% CI 1.03–1.35), were worried about information regarding COVID-19 from the Internet (OR = 1.24; 95% CI 1.08–1.43), persons who were lonely (OR = 1.90; 95% CI 1.54–2.34), and persons with negative problem orientation (OR = 1.26; 95% CI 1.06–1.51). Protective factors were identified as having good self-rated general health (OR = 0.68, 95 % CI 0.58–0.81), maintaining a daily routine (OR = 0.74, 95 % CI 0.61–0.90), having financial stability (OR = 0.66, 95 % CI 0.55–0.79), and having good psychological resilience (OR = 0.90, 95 % CI 0.87–0.94).

Conclusions

This is the first study to report a prevalence of anxiety in the general population of Latvia. Certain factors that predict anxiety, as well as protective factors were identified.
The Associations of Experiencing the COVID-19 Pandemic With Religiosity and Spirituality: A Cross-Sectional Study in Czech Adults
Authors: Buchtova Marie , Malinakova Klara , Novak Lukas , Janu Anna , Husek Vit , Van Dijk Jitse P. , Tavel Peter
Published: June 2022

Objectives: We investigated the associations between religiosity/spirituality and respondents’ changes in their relationships, feelings, thinking, and behaviour during the first wave of the COVID-19 pandemic in the Czech Republic.

Methods: A sample of Czech adults (n = 1,434; 48.3 ± 16.4 years; 49.65% women) participated in the online survey. We measured spirituality, religiosity, self-reported changes in relationships, disrupted feelings, and changes in behaviour during the pandemic.

Results: Spiritual respondents were more likely to report increased physical activity, sex, reading and self-education, with odds ratios (ORs) ranging from 1.26 (95% confidence interval 1.09–1.46) to 1.56 (1.31–1.86). The combination of spirituality and religiosity led to an increase in the range of ORs to 1.57–2.69. Spiritual and religious participants were less likely to feel the decrease of hope by 70%, while mere spirituality significantly reduced the decrease of hope by only 30%. Religiosity itself led to a lower risk of reporting a disrupted day structure with an OR = 0.74 (0.58–0.95).

Conclusion: Religiosity and spirituality separately help people during a pandemic in some areas. Especially their combination has a more positive impact on relationships, feelings, and behaviour.
Safety and Efficacy of Ivermectin for the Prevention and Treatment of COVID-19: A Double-Blinded Randomized Placebo-Controlled Study
Authors: Angkasekwinai Nasikarn , Rattanaumpawan Pinyo , Chayakulkeeree Methee , Phoompoung Pakpoom , Koomanachai Pornpan , Chantarasut Sorawit , Wangchinda Walaiporn , Srinonprasert Varalak , Thamlikitkul Visanu
Published: June 2022

The safety and efficacy of ivermectin for the prevention and treatment of COVID-19 are still controversial topics. From August to November 2021, we conducted a double-blinded, randomized controlled trial at Siriraj Hospital, Thailand. Eligible participants were adults ≥ 18 years with suspected COVID-19 who underwent a SARS-CoV-2 RT-PCR test. After enrollment, the participants were randomized to receive either ivermectin (400–600 µg/kg/d) or placebo once daily for 3 days. Among 983 participants, 536 (54.5%) with a negative RT-PCR result were enrolled in the prevention study, and 447 (45.5%) with a positive RT-PCR result were enrolled in the treatment study. In the prevention study, the incidence of COVID-19 on Day 14 was similar between the ivermectin and the placebo group (4.7% vs. 5.2%; p = 0.844; Δ = −0.4%; 95% CI; −4.3–3.5%). In the treatment study, there was no significant difference between the ivermectin and placebo group for any Day 14 treatment outcome: proportion with oxygen desaturation (2.7% vs. 1.9%; p = 0.75), change in WHO score from baseline (1 [−5, 1] vs. 1 [−5, 1]; p = 0.50), and symptom resolution (76% vs. 82.2%; p = 0.13). The ivermectin group had a significantly higher proportion of transient blurred vision (5.6% vs. 0.6%; p < 0.001). Our study failed to demonstrate the efficacy of a 3-day once daily of ivermectin for the prevention and treatment of COVID-19. The given regimen of ivermectin should not be used for either prevention or treatment of COVID-19 in populations with a high rate of COVID-19 vaccination.
Design and Development of a I4.0 Engineering Education Laboratory with Virtual and Digital Technologies Based on ISO/IEC TR 23842-1 Standard Guidelines
Authors: Cordero-Guridi José-de-Jesús , Cuautle-Gutiérrez Luis , Alvarez-Tamayo Ricardo-Iván , Caballero-Morales Santiago-Omar
Published: June 2022

Knowledge transfer associated with education in the automotive manufacturing and production fields is challenging due to the requirements of physical prototyping of mechanical components and laboratory-assisted testing. In this regard, aspects of Industry 4.0 such as virtual environments and enhanced human–computer interaction have been studied as important resources to improve teaching practices and achieve the equivalent Education 4.0 paradigm. Within the context of modern manufacturing techniques in the Industry 4.0 era and advanced tools for analysis and mechanical design, the present work describes the development of a virtual/augmented reality (VR/AR) laboratory to support learning, training, and collaborative ventures related to additive manufacturing for the automotive industry. The development was performed in accordance with the guidelines of the ISO/IEC TR 23842-1 standard, to ensure that the academic programs and the conditions of use of the laboratory were optimal. Experiences with students through the development of industry-related automotive projects support confidence in the suitability of the laboratory and the expectation of positive outcomes for future developments.
Special Issue on Future Intelligent Transportation System (ITS) for Tomorrow and Beyond
Authors: Nengroo Sarvar Hussain , Jin Hojun , Kim Inhwan , Har Dongsoo
Published: June 2022

Intelligent Transportation System (ITS) has evolved into a system for provision of traffic information and traffic control with the help of advanced IT technologies [...]
Collagen Extraction from Animal Skin
Authors: Matinong Andrea Marie E. , Chisti Yusuf , Pickering Kim L. , Haverkamp Richard G.
Published: June 2022

Collagen is the most abundant structural protein in animals. It is the major component of skin. It finds uses in cosmetics, medicine, yarn production and packaging. This paper reviews the extraction of collagen from hides of most consumed animals for meat with the focus on literature published since 2000. The different pretreatment and extraction techniques that have been investigated for producing collagen from animal skins are reviewed. Pretreatment by enzymatic, acid or alkaline methods have been used. Extraction by chemical hydrolysis, salt solubilization, enzymatic hydrolysis, ultrasound assisted extraction and other methods are described. Post-extraction purification methods are also explained. This compilation will be useful for anyone wishing to use collagen as a resource and wanting to further improve the extraction and purification methods.
Design of a Lab-On-Chip for Cancer Cell Detection through Impedance and Photoelectrochemical Response Analysis
Authors: Hsiao Yu-Ping , Mukundan Arvind , Chen Wei-Chung , Wu Ming-Tsang , Hsieh Shang-Chin , Wang Hsiang-Chen
Published: June 2022

In this study, a biochip was fabricated using a light-absorbing layer of a silicon solar element combined with serrated, interdigitated electrodes and used to identify four different types of cancer cells: CE81T esophageal cancer, OE21 esophageal cancer, A549 lung adenocarcinoma, and TSGH-8301 bladder cancer cells. A string of pearls was formed from dielectrophoretic aggregated cancer cells because of the serrated interdigitated electrodes. Thus, cancer cells were identified in different parts, and electron–hole pairs were separated by photo-excited carriers through the light-absorbing layer of the solar element. The concentration catalysis mechanism of GSH and GSSG was used to conduct photocurrent response and identification, which provides the fast, label-free measurement of cancer cells. The total time taken for this analysis was 13 min. Changes in the impedance value and photocurrent response of each cancer cell were linearly related to the number of cells, and the slope of the admittance value was used to distinguish the location of the cancerous lesion, the slope of the photocurrent response, and the severity of the cancerous lesion. The results show that the number of cancerous cells was directly proportional to the admittance value and the photocurrent response for all four different types of cancer cells. Additionally, different types of cancer cells could easily be differentiated using the slope value of the photocurrent response and the admittance value.
Ischaemic Stroke of the “Hand-Knob” Area Due to Paradoxical Cerebral Air Embolism after Central Venous Catheterization—A Doubly Rare Occurrence: A Case Report and an Overview of Pathophysiology, Diagnosis, and Treatment
Authors: Nicolini Paola , Arighi Andrea , Gherbesi Elisa , Lo Russo Francesco Maria , Mandelli Clara , Schinco Giuseppina , Carugo Stefano , Lucchi Tiziano
Published: June 2022

Central venous catheters (CVCs) are increasingly used across specialties for invasive haemodynamic monitoring and for the delivery of fluids, medications, and nutritional support. Cerebral air embolism (CAE) is a rare but potentially fatal complication associated with the insertion, maintenance, and removal of CVCs. It can occur through different mechanisms, including the direct retrograde ascension of air into the cerebral veins and paradoxical embolism due to a right-to-left intracardiac or intrapulmonary shunt. The “hand-knob” area is the cortical region within the primary motor cortex that contains the representation of the hand. It is located in the superior precentral gyrus and is the site of less than 1% of all ischaemic strokes. We report here the case of a patient who experienced an ischaemic stroke of the right “hand-knob” area, due to paradoxical CAE through a previously undiagnosed patent foramen ovale (PFO), after the insertion of a catheter in the right internal jugular vein. We also provide an overview of the pathophysiology, diagnosis, and treatment of CAE. Suspecting CAE in the case of an acute neurological event occurring in close temporal relationship with central venous catheterization is paramount to allow the early recognition and treatment of this uncommon form of iatrogenic stroke.
Immune Checkpoint Inhibitor Associated Autoimmune Encephalitis, Rare and Novel Topic of Neuroimmunology: A Case Report and Review of the Literature
Authors: Gao Yining , Pan Jie , Shen Dingding , Peng Lisheng , Mao Zhifeng , Wang Chunxia , Meng Huanyu , Zhou Qinming , Chen Sheng
Published: June 2022

Immune checkpoint inhibitors (ICIs) are being used in patients with various advanced malignancies, and patient outcomes have improved considerably. Although ICIs can effectively treat tumors, 30–60% of patients experience immune-related adverse events (irAEs). Autoimmune encephalitis (AE) is a rare irAE that has become a novel topic in neuroimmunology and has received increasing attention in recent years. Herein, we report a rare case of GAD65-antibody–associated AE after metastatic small cell lung cancer treatment with pembrolizumab. The patient received IVIg therapy for AE and continuous pembrolizumab therapy without suspension of tumor treatment. At 1 year follow-up, both the patient’s AE symptoms and tumors were stable. We consider that the treatment of ICI-associated AE should be more individualized with prudent decision-making and should balance the tumor progression and AE treatment. In addition, we have also comprehensively reviewed the literature of ICI-associated AE, and summarized the clinical features, treatment, and prognosis of AE caused by ICI, thus broadening our understanding of the neurological complications caused by ICI.
Exploration of the Common Gene Characteristics and Molecular Mechanism of Parkinson’s Disease and Crohn’s Disease from Transcriptome Data
Authors: Zheng Haoran , Qian Xiaohang , Tian Wotu , Cao Li
Published: June 2022

Parkinson’s disease (PD) is the second most common neurodegenerative disorder, and the mechanism of its occurrence is still not fully elucidated. Accumulating evidence has suggested that the gut acts as a potential origin of PD pathogenesis. Recent studies have identified that inflammatory bowel disease acts as a risk factor for Parkinson’s disease, although the underlying mechanisms remain elusive. The aim of this study was to further explore the molecular mechanism between PD and Crohn’s disease (CD). The gene expression profiles of PD (GSE6613) and CD (GSE119600) were downloaded from the Gene Expression Omnibus (GEO) database and were identified as the common differentially expressed genes (DEGs) between the two diseases. Next, analyses were performed, including functional enrichment analysis, a protein–protein interaction network, core genes identification, and clinical correlation analysis. As a result, 178 common DEGs (113 upregulated genes and 65 downregulated genes) were found between PD and CD. The functional analysis found that they were enriched in regulated exocytosis, immune response, and lipid binding. Twelve essential hub genes including BUB1B, BUB3, DLGAP5, AURKC, CBL, PCNA, RAF1, LYN, RPL39L, MRPL13, RSL24D1, and MRPS11 were identified from the PPI network by using cytoHubba. In addition, inflammatory and metabolic pathways were jointly involved in these two diseases. After verifying expression levels in an independent dataset (GSE99039), a correlation analysis with clinical features showed that LYN and RAF1 genes were associated with the severity of PD. In conclusion, our study revealed the common pathogenesis of PD and CD. These common pathways and hub genes may provide novel insights for mechanism research.
Nanomedicine Penetration to Tumor: Challenges, and Advanced Strategies to Tackle This Issue
Authors: Munir Muhammad Usman
Published: June 2022

Nanomedicine has been under investigation for several years to improve the efficiency of chemotherapeutics, having minimal pharmacological effects clinically. Ineffective tumor penetration is mediated by tumor environments, including limited vascular system, rising cancer cells, higher interstitial pressure, and extra-cellular matrix, among other things. Thus far, numerous methods to increase nanomedicine access to tumors have been described, including the manipulation of tumor micro-environments and the improvement of nanomedicine characteristics; however, such outdated approaches still have shortcomings. Multi-functional convertible nanocarriers have recently been developed as an innovative nanomedicine generation with excellent tumor infiltration abilities, such as tumor-penetrating peptide-mediated transcellular transport. The developments and limitations of nanomedicines, as well as expectations for better outcomes of tumor penetration, are discussed in this review.
Proteomic Profiling Identifies Co-Regulated Expression of Splicing Factors as a Characteristic Feature of Intravenous Leiomyomatosis
Authors: Krasny Lukas , Wilding Chris P. , Perkins Emma , Arthur Amani , Guljar Nafia , Jenks Andrew D. , Fisher Cyril , Judson Ian , Thway Khin , Jones Robin L. , Huang Paul H.
Published: June 2022

Intravenous leiomyomatosis (IVLM) is a rare benign smooth muscle tumour that is characterised by intravenous growth in the uterine and pelvic veins. Previous DNA copy number and transcriptomic studies have shown that IVLM harbors unique genomic and transcriptomic alterations when compared to uterine leiomyoma (uLM), which may account for their distinct clinical behaviour. Here we undertake the first comparative proteomic analysis of IVLM and other smooth muscle tumours (comprising uLM, soft tissue leiomyoma and benign metastasizing leiomyoma) utilising data-independent acquisition mass spectrometry. We show that, at the protein level, IVLM is defined by the unique co-regulated expression of splicing factors. In particular, IVLM is enriched in two clusters composed of co-regulated proteins from the hnRNP, LSm, SR and Sm classes of the spliceosome complex. One of these clusters (Cluster 3) is associated with key biological processes including nascent protein translocation and cell signalling by small GTPases. Taken together, our study provides evidence of co-regulated expression of splicing factors in IVLM compared to other smooth muscle tumours, which suggests a possible role for alternative splicing in the pathogenesis of IVLM.
Research and Application of High-Velocity Oxygen Fuel Coatings
Authors: Yu Jianxing , Liu Xin , Yu Yang , Li Haoda , Liu Pengfei , Huang Kaihang , Sun Ruoke
Published: June 2022

With the development of modern industrial technology, there is an increasingly urgent need for the preparation of high-strength and high-performance coatings on the surface of traditional metal materials [...]
Coexistence of Cor Triatriatum Sinister, Fibroelastoma and Pulmonary Veins Ostial Anatomy Variant as Incidental Findings in Coronary Computed Tomography Angiography
Authors: Gać Paweł , Martuszewski Adrian , Paluszkiewicz Patrycja , Poręba Rafał
Published: June 2022

Coronary computed tomography angiography (CCTA) is a noninvasive examination whose main purpose is to exclude significant stenosis in the coronary arteries. The obtained computed tomography images may also provide information about other coexisting pathologies of the heart and vessels. The paper presents images of cardiac lesions in a 44-year-old hypertensive patient who underwent CCTA, based on which significant stenosis in the coronary arteries was excluded, the suspicion of a cor triatriatum sinister was confirmed and the presence of fibroelastoma and a variant of the anatomy of the pulmonary veins ostial was confirmed. To sum up, when performing CCTA, apart from the analysis of the coronary arteries, one should remember about lesions in the remaining visible anatomical structures of the heart and large vessels.
The Non-Invasive Ultrasound-Based Assessment of Liver Viscosity in a Healthy Cohort
Authors: Popa Alexandru , Sporea Ioan , Bende Felix , Popescu Alina , Fofiu Renata , Borlea Andreea , Bâldea Victor , Pascu Ariana , Foncea Camelia Gianina , Cotrău Radu , Șirli Roxana
Published: June 2022

Liver fibrosis is the most significant prognostic factor in chronic liver disease (CLD). Clinical practice guidelines recommend the use of non-invasive techniques, such as two-dimensional shear-wave elastography (2D-SWE), to assess liver stiffness as a marker of fibrosis. Several other factors influence liver stiffness in addition to liver fibrosis. It is presumed that changes due to necro-inflammation modify the propagation of shear waves (dispersion). Therefore, new imaging techniques that investigate the dispersion properties of shear waves have been developed, which can serve as an indirect method of measuring liver viscosity (Vi PLUS). Defining the reference values in healthy subjects among different age groups and genders and analyzing the factors that influence these values is essential. However, published data on liver viscosity are still limited. This is the first study that aimed to assess the normal range of liver viscosity values in subjects with healthy livers and analyze the factors that influence them. One hundred and thirty-one consecutive subjects with healthy livers were enrolled in this prospective study. The results showed that Vi PLUS is a highly feasible method. Liver stiffness, age and BMI influenced the liver viscosity values. The mean liver viscosity by Vi PLUS in subjects with healthy livers was 1.59 Pa·s.
Estimation of Factors Affecting Burnout in Greek Dentists before and during the COVID-19 Pandemic
Authors: Antoniadou Maria
Published: June 2022

This study is a comprehensive, cross-sectional survey in occupational burnout, career satisfaction, and quality of life conducted in March 2021 among dentists in the vast area of metropolitan Athens, Greece. Data were collected using a self-reported questionnaire based on the Copenhagen Questionnaire (CQ) for assessing work stress and the Maslach Burnout Inventory-Human Service Survey (MBI-HSS) for evaluating occupational burnout. Using the independent t-test, ANOVA, Pearson’s correlation, and multiple linear regression, 804 valid questionnaires were analyzed. During the pandemic, personal exhaustion was affected by gender (b = 1.862, p = 0.0001), age (b = −0.598, p = 0.0001), number of children (b = −0.886, p = 0.020) and higher degree (b = −0.450, p = 0.012). Exhaustion due to working with patients was affected by gender (b = 0.662 p = 0.0001), age (b = −0.513, p = 0.0001), number of children (b = −0.701, p = 0.0001), higher degree (b = −0.207, p = 0.028) and years in practice (b = 0.408 p = 0.0001). Males were more prone to unhappiness, dissatisfaction, and professional physical and emotional exhaustion, but personal resources through higher education, beliefs, values, and hobbies can offer a preventive shield to all dental professionals. Economic management issues can also enhance dentists’ satisfaction and feeling of safety in a rapidly changing environment.
Advances in Flipped Classrooms for Teaching and Learning Forensic Geology
Authors: Somma Roberta
Published: June 2022

One of the most famous criminal investigations involved the use of forensic geology to assist law enforcement agencies in the homicide case of the Italian honorable Aldo Moro. Notwithstanding this important tribute to forensic geology, in Italy, the role and value of using geological and soil materials (known as earth materials) to support law enforcement agencies in solving criminal investigations remain uncommon. This absence may be due to few educational courses for geology undergraduates/graduates devoted to laboratory and field training in forensic geology. The flipped classroom model may encourage a modern educational approach for teaching and learning forensic geology. The designed flipped classroom model applies theoretical concepts for forensic geology, which is learned by the attendees at home, whereas the class activities are devoted to laboratory and field experiences assisted by teaching staff. The laboratory activities involve techniques for collecting geological trace evidence and comparing color/sedimentological/mineralogical/microfossil features, whereas the field experiences consist of sampling strategies, search activities for burials, and field surveying. This approach has been trialed by the Messina University since 2014 and represents a successful tool for multitasking teaching and learning aimed to further develop forensic geology, encourage the inclusion of forensic geologists within the police enforcement in Italy, and improve the knowledge of law experts such as prosecutors and defense lawyers.
Hybrid Quasi-Optimal PID-SDRE Quadrotor Control
Authors: Giernacki Wojciech , Stępień Sławomir , Chodnicki Marcin , Wróblewska Agnieszka
Published: June 2022

In the paper, a new cascade control system for an autonomous flight of an unmanned aerial vehicle (UAV) based on Proportional–Integral–Derivative (PID) and finite-time State-Dependent Riccati Equation (SDRE) control is proposed. The PID and SDRE controllers are used in a hybrid control system for precise control and stabilization, which is necessary to increase the drone’s flight stability and maneuver precision. The hybrid PID-SDRE control system proposed for the quadrotor model is quasi-optimal, since the suboptimal control algorithm for the UAV stabilization is used. The combination of the advantages of PID and SDRE control gives a significant improvement in the quality of control while maintaining the simplicity of the control system. Furthermore, the use of the suboptimal control technique provides the UAV attitude tracking in finite time. These remarks are drawn from a series of simulation tests conducted for the drone model.