bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of Non-Contact Measurement Techniques for Concrete Elements Using Light Detection and Ranging
Authors: Pham Thanh Thi , Kim Doyun , Woo Ukyong , Jeong Su-Gwang , Choi Hajin
Published: December 2023

The objective of this study is to develop a monitoring algorithm that measures the displacement of concrete structures using light detection and ranging (LiDAR). The suggested method is based on non-contact measurements providing 3D point clouds of the scanning area with high resolution. This overcomes the limitation of traditional contact-type and point-based measurement methods such as linear variable differential transformer (LVDT) and strain gauge. The developed algorithm enables one to track the boundaries of a concrete specimen and measures the vertical or lateral displacement. To demonstrate that displacement in the horizontal and vertical direction can be measured irrespective of the field of view (FOV), two different concrete specimens were constructed where gradually increasing vertical or lateral loads were applied. Then, the displacements were monitored using the set of LVDT and LiDAR for the correlation analysis. The results demonstrated a high accuracy of 98~99% correlation in comparison between LVDT and LiDAR.
Renewability and Robustness Analysis and Review for Sustainable-Technology Propulsion Systems in Modern Transportation Infrastructure Administration
Authors: Covaci Mihnea-Antoniu , Gălătuș Ramona Voichița , Petreuș Dorin Marius , Szolga Lorant Andras
Published: December 2023

Recently, major climate events highlighted the increasing need to use sustainable technologies in the transportation domain. Energy production infrastructure, storage, and propulsion systems still rely on non-sustainable technology for economic growth. Therefore, this study reviews the modern transportation propulsion systems and transportation infrastructure components, describing the possible outcomes for several future directions based on prototypes and study advances. The in-production vehicles were reviewed for providing immediate, robust, and renewable solutions for the existing non-sustainable transportation infrastructure. The study continues with extended-capability vehicles and their limitations and vulnerability based on the current infrastructural circumstances. An alternative energy transfer infrastructure has been concluded to possibly provide the necessary capabilities to approach a neutral carbon footprint and mitigate ongoing climate adverse events. The hypothetical prototype uses distance energy transfer to bypass the described environmental constraints and provide a direction for achieving a possibly sustainable and economically evolving infrastructure.
Flow around an Aircraft Model—Comparison between Hydrodynamic Tunnel Tests and Computational Fluid Dynamics Simulations
Authors: Mikołajczyk Anna , Dziubiński Adam , Kurnyta-Mazurek Paulina , Kachel Stanisław
Published: December 2023

This paper presents a comparative analysis of the results obtained using the computational fluid dynamic method and a hydrodynamic tunnel. The aerodynamic properties of the General Dynamics F-111 Aardvark aircraft were subjected to both qualitative and quantitative assessment. In both cases, the same geometric model of the aircraft was used. For the hydrodynamic tunnel tests, the model was 3D printed, while for the CFD simulations, the geometry was imported into ANSYS Fluent 2021 R2 software. During quantitative studies, aerodynamic characteristics, including the coefficients of lift, drag, and pitching moment for the aircraft, were determined over a wide range of angles of attack. In turn, flow field images and pathline visualization of the vortex were obtained following qualitative studies. The comparative analysis showed that high consistency between the simulation and experimental results was achieved.
The Molecular Detection and Antimicrobial Profiles of Selected Bacterial Pathogens in Slaughterhouses in Riyadh City, Saudi Arabia
Authors: Albuqami Shujaa A. , Dawoud Turki M. , Moussa Ihab Mohamed , Elbehiry Ayman , Alsubki Roua A. , Hemeg Hassan A. , Qattan Malak Yahia , Alhaji Jwaher H.
Published: December 2023

Inadequate hygienic conditions and poor handling are the primary causes of contamination in abattoirs. This study aimed to identify and molecularly detect pathogenic bacteria in sheep meat at slaughterhouses in Riyadh City, Saudi Arabia. Additionally, the study evaluated the sensitivity of these bacteria to various antimicrobials. In total, 150 samples were collected three times every two months from November 2021 to March 2022 from four abattoirs located in the south, west, east, and north of Riyadh. Pathogenic bacteria were separated using selective media, and the Vitek 2 system was utilized to identify all species and test their response to antibiotics. PCR was employed to detect virulence genes. The four pathogenic bacteria identified in all samples were Escherichia coli (12%), Klebsiella pneumoniae (9.3%), Salmonella enterica (7.3%), and Pseudomonas aeruginosa (6.6%). Abattoir D had a high number of bacteria isolated in January. K. pneumoniae and S. enterica exhibited resistance to ampicillin. S. enterica also demonstrated resistance to gentamicin, ciprofloxacin, and trimethoprim/sulfamethoxazole. P. aeruginosa was resistant to tigecycline. PCR results indicated positive tests for the E. coli gene FimH, the P. aeruginosa genes plcH and toxA, and the K. pneumoniae gene mrkD. Pathogenic bacteria with positive results for virulence genes have the potential to cause contamination and human diseases. To improve quality and reduce contamination, the government must address the issue of providing adequate and safe water for activities in all abattoirs in Riyadh City.
Air Quality Characterization and Trend Analysis in a Brazilian Industrialized Metropolitan Area in the Period from 1995 to 2022
Authors: Ribeiro Amanda Karine Chaves , Galvão Elson Silva , Albuquerque Taciana Toledo de Almeida
Published: December 2023

The Brazilian population grew approximately 9% from 2006 to 2016, and the number of deaths caused by air pollution increased by 14% in Brazil in the same period. Facing the lack of studies on air quality in the Metropolitan Area of Belo Horizonte (MABH)—the third most populous Brazilian metropolitan area—this study aimed to investigate the air quality and the trends of air pollutant concentrations in the MABH between 1995 and 2022, using data from the air quality monitoring network. The methodology consisted of checking MABHs air quality trends following the WHO air quality guidelines. The Mann–Kendall test was used to check statistically for the possibility of tendencies. The results showed a trend of stability in the concentrations of air pollutants in the MABH without any trend of improvement or worsening. However, the time series of the MABH exposed the challenge of ensuring better air quality that protects human health. Furthermore, the results reinforced the importance of focusing on pollutant sources and exposed the need for improvements in air quality management. Thus, it is essential to reverse the current dismantling scenario of the public environmental agencies in Brazil.
Remarks on Conjectures in Block Theory of Finite Groups
Authors: Algreagri Manal H. , Alghamdi Ahmad M.
Published: December 2023

In this paper, we focus on Brauer’s height zero conjecture, Robinson’s conjecture, and Olsson’s conjecture regarding the direct product of finite groups and give relative versions of these conjectures by restricting them to the algebraic concept of the anchor group of an irreducible character. Consider G to be a finite simple group. We prove that the anchor group of the irreducible character of G with degree p is the trivial group, where p is an odd prime. Additionally, we introduce the relative version of the Green correspondence theorem with respect to this group. We then apply the relative versions of these conjectures to suitable examples of simple groups. Classical and standard theories on the direct product of finite groups, block theory, and character theory are used to achieve these results.
Improvement of Metal-Doped β-TCP Scaffolds for Active Bone Substitutes via Ultra-Short Laser Structuring
Authors: Soares Íris , Sotelo Lamborghini , Erceg Ina , Jean Florian , Lasgorceix Marie , Leriche Anne , Sikirić Maja Dutour , Marušić Katarina , Christiansen Silke , Daskalova Albena
Published: December 2023

Various efforts have been made to develop antibacterial biomaterials capable of also sustaining bone remodulation to be used as bone substitutes and reduce patient infection rates and related costs. In this work, beta-tricalcium phosphate (β-TCP) was chosen due to its known biocompatibility and use as a bone substitute. Metal dopants were incorporated into the crystal structure of the β-TCP, and disks were produced from this material. Magnesium and strontium, as well as copper and silver, were chosen as dopants to improve the osteogenic and antibacterial properties, respectively. The surface of the β-TCP samples was further modified using a femtosecond laser system. Grid and line patterns were produced on the plates’ surface via laser ablation, creating grooves with depths lower than 20 μm and widths between 20 and 40 μm. Raman and FTIR analysis confirmed that laser ablation did not result in the degradation or phase change of the materials, making it suitable for surface patterning. Laser ablation resulted in increased hydrophilicity of the materials, as the control samples (non-ablated samples) have WCA values ranging from 70° to 93° and become, upon laser ablation, superwicking surfaces. Confocal measurements show an increase in specific surface area of 50% to 200% compared to the control. Overall, the results indicate the potential of laser ablation to improve the surface characteristics of β-TCP, which may lead to an improvement in the antibacterial and osteogenic properties of the produced materials.
Reliability of Systematic and Targeted Biopsies versus Prostatectomy
Authors: Guan Tianyuan , Sidana Abhinav , Rao Marepalli B.
Published: December 2023

Systematic Biopsy (SBx) has been and continues to be the standard staple for detecting prostate cancer. The more expensive MRI guided biopsy (MRITBx) is a better way of detecting cancer. The prostatectomy can provide an accurate condition of the prostate. The goal is to assess how reliable SBx and MRITBx are vis à vis prostatectomy. Graded Gleason scores are used for comparison. Cohen’s Kappa index and logistic regression after binarization of the graded Gleason scores are some of the methods used to achieve our goals. Machine learning methods, such as classification trees, are employed to improve predictability clinically. The Cohen’s Kappa index is 0.31 for SBx versus prostatectomy, which means a fair agreement. The index is 0.34 for MRITBx versus prostatectomy, which again means a fair agreement. A direct comparison of SBx versus prostatectomy via binarized graded scores gives sensitivity 0.83 and specificity 0.50. On the other hand, a direct comparison of MRITBx versus prostatectomy gives sensitivity 0.78 and specificity 0.67, putting MRITBx on a higher level of accuracy. The SBx and MRITBx do not yet match the findings of prostatectomy completely, but they are useful. We have developed new biomarkers, considering other pieces of information from the patients, to improve the accuracy of SBx and MRITBx. From a clinical point of view, we provide a prediction model for prostatectomy Gleason grades using classification tree methodology.
Training Postural Balance Control with Pelvic Force Field at the Boundary of Stability
Authors: Omofuma Isirame , Santamaria Victor , Ai Xupeng , Agrawal Sunil
Published: December 2023

This study characterizes the effects of a postural training program on balance and muscle control strategies in a virtual reality (VR) environment. The Robotic Upright Stand Trainer (RobUST), which applies perturbative forces on the trunk and assistive forces on the pelvis, was used to deliver perturbation-based balance training (PBT) in a sample of 10 healthy participants. The VR task consisted of catching, aiming, and throwing a ball at a target. All participants received trunk perturbations during the VR task with forces tailored to the participant’s maximum tolerance. A subgroup of these participants additionally received assistive forces at the pelvis during training. Postural kinematics were calculated before and after RobUST training, including (i) maximum perturbation force tolerated, (ii) center of pressure (COP) and pelvic excursions, (iii) postural muscle activations (EMG), and (iv) postural control strategies (the ankle and hip strategies). We observed an improvement in the maximum perturbation force and postural stability area in both groups and decreases in muscle activity. The behavior of the two groups differed for perturbations in the posterior direction where the unassisted group moved towards greater use of the hip strategy. In addition, the assisted group changed towards a lower margin of stability and higher pelvic excursion. We show that training with force assistance leads to a reactive balance strategy that permits pelvic excursion but that is efficient at restoring balance from displaced positions while training without assistance leads to reactive balance strategies that restrain pelvic excursion. Patient populations can benefit from a platform that encourages greater use of their range of motion.
The Influences of Rainfall Intensity and Timing on the Assemblage of Dung Beetles and the Rate of Dung Removal in an Alpine Meadow
Authors: Sun Wenxiao , Tang Wenting , Wu Yashi , He Shuaibing , Wu Xinwei
Published: December 2023

Changes in precipitation patterns, including rainfall intensity and rainfall timing, have been extensively demonstrated to impact biological processes and associated ecosystem functions. However, less attention has been paid to the effects of rainfall intensity and rainfall timing on the assembly of detritivore communities and the decomposition rate of detritus such as animal dung. In a grazed alpine meadow on the eastern Qinghai–Tibet Plateau, we conducted a manipulative experiment involving two levels of rainfall intensity (heavy rainfall, 1000 mL/5 min; light rainfall, 100 mL/5 min) and five levels of rainfall timing (0, 2, 4, 24, and 48 h after yak dung deposition). The aim was to determine the effects of rainfall intensity, timing, and their interaction on the assemblage of dung beetles and dung removal rate during the early stage (i.e., 96 h after yak dung deposition) of dung decomposition. Light rainfall significantly increased species richness in the treatment of 48 h after dung pats were deposited. Heavy rainfall significantly decreased beetle abundance in both the 0 h and 48 h treatments while light rainfall had no effect on beetle abundance. Dung mass loss was significant lower in the 2 h treatment compared to other treatments regardless of rainfall intensity. The structural equation model further revealed that the species richness of dung beetles and dung mass loss were significantly affected by rainfall timing but not by rainfall intensity. However, no significant relationships were observed between any variables examined. These findings suggest that changes in precipitation patterns can influence both the structure of dung beetles and the rate of dung decomposition but may also decouple their relationship under a certain circumstance. Therefore, it is crucial to pay greater attention to fully understand local variability between the biological processes and ecosystem functions within a global climate change scenario.
Integrin α6β4 Confers Doxorubicin Resistance in Cancer Cells by Suppressing Caspase-3–Mediated Apoptosis: Involvement of N-Glycans on β4 Integrin Subunit
Authors: Kariya Yoshinobu , Gu Jianguo , Kariya Yukiko
Published: December 2023

Drug resistance is a major obstacle to successful cancer treatment. Therefore, it is essential to understand the molecular mechanisms underlying drug resistance to develop successful therapeutic strategies. α6β4 integrin confers resistance to apoptosis and regulates the survival of cancer cells; however, it remains unclear whether α6β4 integrin is directly involved in chemoresistance. Here, we show that α6β4 integrin promotes doxorubicin resistance by decreasing caspase-3–mediated apoptosis. We found that the overexpression of α6β4 integrin by the β4 integrin gene rendered MDA-MB435S and Panc-1 cells more resistant to doxorubicin than control cells. The acquired resistance to doxorubicin by α6β4 integrin expression was abolished by the deletion of the cytoplasmic signal domain in β4 integrin. Similar results were found in MDA-MB435S and Panc-1 cells when N-glycan-defective β4 integrin mutants were overexpressed or bisecting GlcNAc residues were increased on β4 integrin by the co-expression of N-acetylglucosaminyltransferase III with β4 integrin. The abrogation of α6β4 integrin-mediated resistance to doxorubicin was accompanied by reduced cell viability and an increased caspase-3 activation. Taken together, our results clearly suggest that α6β4 integrin signaling plays a key role in the doxorubicin resistance of cancer cells, and N-glycans on β4 integrin are involved in the regulation of cancer cells.
Fatty-Acid-Binding Proteins: From Lipid Transporters to Disease Biomarkers
Authors: Gaffar Shabarni , Aathirah A Sayyidatina
Published: December 2023

Fatty-acid-binding proteins (FABPs) serve a crucial role in the metabolism and transport of fatty acids and other hydrophobic ligands as an intracellular protein family. They are also recognized as a critical mediator in the inflammatory and ischemic pathways. FABPs are found in a wide range of tissues and organs, allowing them to contribute to various disease/injury developments that have not been widely discussed. We have collected and analyzed research journals that have investigated the role of FABPs in various diseases. Through this review, we discuss the findings on the potential of FABPs as biomarkers for various diseases in different tissues and organs, looking at their expression levels and their roles in related diseases according to available literature data. FABPs have been reported to show significantly increased expression levels in various tissues and organs associated with metabolic and inflammatory diseases. Therefore, FABPs are a promising novel biomarker that needs further development to optimize disease diagnosis and prognosis methods along with previously discovered markers.
Biohybrid Nanoparticle-Based In Situ Monitoring of In Vivo Drug Delivery
Authors: Ju Sohee , Cho Hyeon-Yeol
Published: December 2023

Nanomaterials have gained huge attention worldwide owing to their unique physicochemical characteristics which enable their applications in the field of biomedicine and drug delivery systems. Although nanodrug delivery systems (NDDSs) have better target specificity and bioavailability than traditional drug delivery systems, their behavior and clearance mechanisms in living subjects remain unclear. In this regard, the importance of bioimaging methods has come to the forefront for investigating the biodistribution of nanocarriers and discovering drug release mechanisms in vivo. In this review, we introduce several examples of biohybrid nanoparticles and their clinical applications, focusing on their advantages and limitations. The various bioimaging methods for monitoring the fate of nanodrugs in biological systems and the future perspectives of NDDSs have also been discussed.
Review of Recent Developments Regarding the Durability Performance of Eco-Friendly Geopolymer Concrete
Authors: Alahmari Turki S. , Abdalla Tareg Abdalla , Rihan Mohammed Ali M.
Published: December 2023

The 21st century has witnessed a substantial increase in the demand for construction materials, mainly influenced by the growing population. This increase in demand has resulted in higher prices for these materials and has also placed considerable burdens on environmental resources, prompting the search for eco-friendly and economically viable alternatives such as geopolymer materials to replace traditional materials like cement. The benefits of geopolymer materials as substitutes for cement in concrete extend beyond their exceptional durability. Initially, geopolymer was introduced to address the environmental impact arising from carbon dioxide emissions and the substantial consumption of fossil fuels through the production of cement. The current review investigates recent advances regarding the durability characteristics of geopolymer materials. This includes aspects such as water absorption, temperature resistance, sulfuric acid resistance, sulfate resistance, chloride ion penetration, and freeze–thaw resistance, among others. The results of this review highlight geopolymer concrete’s enhanced durability over traditional cement-based concrete. Furthermore, this review offers recommendations and outlines potential research avenues for further exploration of geopolymer concrete.
The Genomic Landscape of Urothelial Carcinoma with High and Low ERBB2 Expression
Authors: Hadadi Agreen , Krause Harris B. , Elliott Andrew , Brown Jacqueline T. , Nazha Bassel , Harik Lara R. , Carthon Bradley C. , Miron Benjamin , Nabhan Chadi , Barata Pedro C. , Saleh Mohamed , Yang Yuanquan , McKay Rana R. , Bilen Mehmet A.
Published: December 2023

Background: Recent data suggests that HER2-targeted treatment is efficacious in urothelial carcinoma (UC). We investigated the genomic, transcriptomic, and immune landscapes and clinical outcomes in UC segmented by ERBB2 expression. Methods: NextGen DNA/RNA sequencing was performed for 4743 UC tumors. A total of 3% (124/4125) of tumors had HER2 IHC and whole transcriptome sequencing (WTS) data. ERRB2-high and -low tumors were defined by ≥75th and <25th percentiles of ERBB2 expression, respectively. PD-L1 (SP142) positive staining was defined as ≥2+ and ≥5%. HER2 (4B5) positive staining was defined as ≥3+ and >10% or 2+ and >10% with positive HER2 in situ hybridization (ISH). Results: Of the patients who were ERBB2-high, 79% (61/77) were HER2 positive via IHC. Tumors from lower tract UC had higher ERBB2 expression compared to upper tract UC (50 v 40 median TPM (mTPM), p < 0.001). ERBB2 expression was similar between primary and metastatic tumors (47 v 47 mTPM, p = 0.95). ERBB2-high tumors had a higher prevalence of pathogenic mutations in pTERT, ERBB2, and ELF3 versus ERBB2-low tumors, p < 0.001. ERBB2-high tumors had higher expressions of ADC target genes NECTIN4 (12 v 8 mTPM) and TACSTD2 (366 v 74 mTPM) versus ERBB2-low (p < 0.001), as well as better overall survival from time of tissue sampling than ERBB2-low (HR 1.71, p < 0.001). Conclusion: Our study demonstrated a high concordance between HER2 expression by IHC and ERBB2 gene expression by WTS in UC. Differences in ADC target expression between ERBB2-high vs. ERBB2-low UC may provide a rationale for combination treatment strategies with HER2-ADC. The association between high ERBB2 expression and survival advantage warrants further investigation.
Proton Reirradiation for High-Risk Recurrent or New Primary Breast Cancer
Authors: Chakraborty Molly A. , Khan Atif J. , Cahlon Oren , Xu Amy J. , Braunstein Lior Z. , Powell Simon N. , Choi J. Isabelle
Published: December 2023

Radiotherapy is an integral component of multidisciplinary breast cancer care. Given how commonly radiotherapy is used in the treatment of breast cancer, many patients with recurrences have received previous radiotherapy. Patients with new primary breast cancer may also have received previous radiotherapy to the thoracic region. Curative doses and comprehensive field photon reirradiation (reRT) have often been avoided in these patients due to concerns for severe toxicities to organs-at-risk (OARs), such as the heart, lungs, brachial plexus, and soft tissue. However, many patients may benefit from definitive-intent reRT, such as patients with high-risk disease features such as lymph node involvement and dermal/epidermal invasion. Proton therapy is a potentially advantageous treatment option for delivery of reRT due to its lack of exit dose and greater conformality that allow for enhanced non-target tissue sparing of previously irradiated tissues. In this review, we discuss the clinical applications of proton therapy for patients with breast cancer requiring reRT, the currently available literature and how it compares to historical photon reRT outcomes, treatment planning considerations, and questions in this area warranting further study. Given the dosimetric advantages of protons and the data reported to date, proton therapy is a promising option for patients who would benefit from the added locoregional disease control provided by reRT for recurrent or new primary breast cancer.
Beta-Hydroxybutyrate Augments Oxaliplatin-Induced Cytotoxicity by Altering Energy Metabolism in Colorectal Cancer Organoids
Authors: Sever Tolga , Ellidokuz Ender Berat , Basbinar Yasemin , Ellidokuz Hulya , Yilmaz Ömer H. , Calibasi-Kocal Gizem
Published: December 2023

Deregulation of cellular metabolism has recently emerged as a notable cancer characteristic. This reprogramming of key metabolic pathways supports tumor growth. Targeting cancer metabolism demonstrates the potential for managing colorectal cancer. Beta-hydroxybutyrate (BOHB) acts as an acetyl-CoA source for the tricarboxylic acid (TCA) cycle, possibly redirecting energy metabolic pathways towards the TCA cycle that could enhance sensitivity to oxaliplatin, through the generation of reactive oxygen species (ROS). This study explores the potential of BOHB to enhance oxaliplatin’s cytotoxic effect by altering the energy metabolism in colorectal cancer. The study employed advanced in vitro organoid technology, which successfully emulates in vivo physiology. The combination treatment efficacy of BOHB and oxaliplatin was evaluated via cell viability assay. The levels of key proteins involved in energy metabolism, apoptotic pathways, DNA damage markers, and histone acetylation were analyzed via Western Blot. ROS levels were evaluated via flow cytometer. Non-toxic doses of BOHB with oxaliplatin significantly amplified cytotoxicity in colorectal cancer organoids. Treatment with BOHB and/or melatonin resulted in significantly decreased lactate dehydrogenase A and increased mitochondrial carrier protein 2 levels, indicating inhibited aerobic glycolysis and an increased oxidative phosphorylation rate. This metabolic shift induced apoptotic cell death mediated by oxaliplatin, owing to high levels of ROS. Melatonin counteracted this effect by protecting cancer cells from high oxidative stress conditions. BOHB may enhance the efficacy of chemotherapeutics with a similar mechanism of action to oxaliplatin in colorectal cancer treatment. These innovative combinations could improve treatment outcomes for colorectal cancer patients.
“Things Have Changed”—Laparoscopic Cytoreduction for Advanced and Recurrent Ovarian Cancer: The Experience of a Referral Center on 108 Patients
Authors: Ceccaroni Marcello , Roviglione Giovanni , Bruni Francesco , Dababou Susan , Venier Martina , Zorzi Carlotta , Salgarello Matteo , Ruffo Giacomo , Alongi Filippo , Gori Stefania , Driul Lorenza , Uccella Stefano , Barra Fabio
Published: December 2023

Objective: To report the feasibility of laparoscopic cytoreduction surgery for primary and recurrent ovarian cancer in a select group of patients. Methods: A retrospective analysis was conducted on a cohort of patients with FIGO stage IIIA-IV advanced ovarian cancer who underwent laparoscopic primary debulking surgery (PDS), interval debulking surgery (IDS), or secondary debulking surgery (SDS) between June 2008 and January 2020. The primary endpoint was achieving optimal cytoreduction, defined as residual tumor less than 1 cm. Secondary endpoints included evaluating surgical complications and long-term survival, assessed at three-month intervals during the initial two years and then every six months. Results: This study included a total of 108 patients, among whom, 40 underwent PDS, 44 underwent IDS, and 24 underwent SDS. Optimal cytoreduction rates were found to be 95.0%, 97.7%, and 95.8% for the PDS, ISD, and SDS groups, respectively. Early postoperative complications (<30 days from surgery) occurred in 19.2% of cases, with 7.4% of these cases requiring reintervention. One patient died following postoperative respiratory failure. Late postoperative complications (<30 days from surgery) occurred in 9.3% of cases, and they required surgical reintervention only in one case. After laparoscopic optimal cytoreduction with a median follow-up time of 25 months, the overall recurrence rates were 45.7%, 38.5%, and 39.3% for PDS, ISD, and SDS, respectively. The three-year overall survival rates were 84%, 66%, and 63%, respectively, while the three-year disease-free survival rates were 48%, 51%, and 71%, respectively. Conclusions: Laparoscopic cytoreduction surgery is feasible for advanced ovarian cancer in carefully selected patients, resulting in high rates of optimal cytoreduction, satisfactory peri-operative morbidity, and encouraging survival outcomes. Future studies should focus on establishing standardized selection criteria and conducting well-designed investigations to further refine patient selection and evaluate long-term outcomes.
Inhibition of DHODH Enhances Replication-Associated Genomic Instability and Promotes Sensitivity in Endometrial Cancer
Authors: Zhao Shengyuan , Francois Aaliyah , Kidane Dawit
Published: December 2023

Endometrial carcinoma (EC) is the most common gynecological malignancy in the United States. De novo pyrimidine synthesis pathways generate nucleotides that are required for DNA synthesis. Approximately 38% of human endometrial tumors present with an overexpression of human dihydroorotate dehydrogenase (DHODH). However, the role of DHODH in cancer cell DNA replication and its impact on modulating a treatment response is currently unknown. Here, we report that endometrial tumors with overexpression of DHODH are associated with a high mutation count and chromosomal instability. Furthermore, tumors with an overexpression of DHODH show significant co-occurrence with mutations in DNA replication polymerases, which result in a histologically high-grade endometrial tumor. An in vitro experiment demonstrated that the inhibition of DHODH in endometrial cancer cell lines significantly induced replication-associated DNA damage and hindered replication fork progression. Furthermore, endometrial cancer cells were sensitive to the DHODH inhibitor either alone or in combination with the Poly (ADP-ribose) polymerase 1 inhibitor. Our findings may have important clinical implications for utilizing DHODH as a potential target to enhance cytotoxicity in high-grade endometrial tumors.
Levels of Cell-Free DNA in Kidney Failure Patients before and after Renal Transplantation
Authors: Leotta Chiara , Hernandez Leah , Tothova Lubomira , Arefin Samsul , Ciceri Paola , Cozzolino Mario Gennaro , Barany Peter , Chromek Milan , Stenvinkel Peter , Kublickiene Karolina
Published: December 2023

Circulating cell-free DNA (cfDNA) has diverse applications in oncological, prenatal, toxicological, cardiovascular, and autoimmune diseases, diagnostics, and organ transplantation. In particular, mitochondrial cfDNA (mt-cfDNA) is associated with inflammation and linked to early vascular ageing (EVA) in end-stage kidney failure (ESKF), which could be a noninvasive marker for graft rejection and organ damage. Plasma samples from 44 ESKF patients, of whom half (n = 22) underwent either conservative therapy (non-HD) or hemodialysis (HD) before kidney transplantation (KT). These samples were analyzed at baseline and two years after KT. cfDNA was extracted from plasma and quantified using the fluorometric method. qPCR was used to quantify and differentiate the fractions of mt-cfDNA and nuclear cfDNA (nc-cfDNA). mt-cfDNA levels in KT patients decreased significantly from baseline to two years post-KT (p < 0.0268), while levels of total cfDNA and nc-cfDNA did not differ. Depending on therapy modality (HD vs. non-HD) before KT, total cfDNA levels were higher in HD patients at both baseline (p = 0.0133) and two years post-KT (p = 0.0421), while nc-cfDNA levels were higher in HD only at baseline (p = 0.0079). Males showed a nonsignificant trend of higher cfDNA levels. Patients with assessed vascular fibrosis (p = 0.0068), either alone or in combination with calcification plus fibrosis, showed reduced mt-cfDNA post-KT (p = 0.0195). Changes in mt-cfDNA levels suggests the impact of KT on the inflammatory state of ESKF, as evidenced via its correlation with high sensitivity C-reactive protein after KT. Further studies are warranted to assess if cfDNA could serve as a noninvasive method for monitoring the response to organ transplantation and even for amelioration of EVA status per se.
New Perspectives in Radiological and Radiopharmaceutical Hybrid Imaging in Progressive Supranuclear Palsy: A Systematic Review
Authors: Strobel Joachim , Müller Hans-Peter , Ludolph Albert C. , Beer Ambros J. , Sollmann Nico , Kassubek Jan
Published: December 2023

Progressive supranuclear palsy (PSP) is a neurodegenerative disease characterized by four-repeat tau deposition in various cell types and anatomical regions, and can manifest as several clinical phenotypes, including the most common phenotype, Richardson’s syndrome. The limited availability of biomarkers for PSP relates to the overlap of clinical features with other neurodegenerative disorders, but identification of a growing number of biomarkers from imaging is underway. One way to increase the reliability of imaging biomarkers is to combine different modalities for multimodal imaging. This review aimed to provide an overview of the current state of PSP hybrid imaging by combinations of positron emission tomography (PET) and magnetic resonance imaging (MRI). Specifically, combined PET and MRI studies in PSP highlight the potential of [18F]AV-1451 to detect tau, but also the challenge in differentiating PSP from other neurodegenerative diseases. Studies over the last years showed a reduced synaptic density in [11C]UCB-J PET, linked [11C]PK11195 and [18F]AV-1451 markers to disease progression, and suggested the potential role of [18F]RO948 PET for identifying tau pathology in subcortical regions. The integration of quantitative global and regional gray matter analysis by MRI may further guide the assessment of reduced cortical thickness or volume alterations, and diffusion MRI could provide insight into microstructural changes and structural connectivity in PSP. Challenges in radiopharmaceutical biomarkers and hybrid imaging require further research targeting markers for comprehensive PSP diagnosis.
Scanning Probe Microscopy Techniques for Studying the Cell Glycocalyx
Authors: Kolesov Dmitry , Astakhova Anna , Galdobina Maria , Moskovtsev Alexey , Kubatiev Aslan , Sokolovskaya Alisa , Ukrainskiy Leonid , Morozov Sergey
Published: December 2023

The glycocalyx is a brush-like layer that covers the surfaces of the membranes of most cell types. It consists of a mixture of carbohydrates, mainly glycoproteins and proteoglycans. Due to its structure and sensitivity to environmental conditions, it represents a complicated object to investigate. Here, we review studies of the glycocalyx conducted using scanning probe microscopy approaches. This includes imaging techniques as well as the measurement of nanomechanical properties. The nanomechanics of the glycocalyx is particularly important since it is widely present on the surfaces of mechanosensitive cells such as endothelial cells. An overview of problems with the interpretation of indirect data via the use of analytical models is presented. Special insight is given into changes in glycocalyx properties during pathological processes. The biological background and alternative research methods are briefly covered.
A Mouse Model to Study the Pathogenesis of γ-herpesviral Infections in Germinal Center B Cells
Authors: Rambold Ursula , Sperling Stefanie , Chew Zakir , Wang Yan , Steer Beatrix , Zeller Krisztina , Strobl Lothar J. , Zimber-Strobl Ursula , Adler Heiko
Published: December 2023

CD30-positive germinal center (GC)-derived B cell lymphomas are frequently linked to Epstein–Barr Virus (EBV) infection. However, a suitable animal model for the investigation of the interplay between γ-herpesvirus and host cells in B cell pathogenesis is currently lacking. Here, we present a novel in vivo model enabling the analysis of genetically modified viruses in combination with genetically modified GC B cells. As a murine γ-herpesvirus, we used MHV-68 closely mirroring the biology of EBV. Our key finding was that Cre-mediated recombination can be successfully induced by an MHV-68 infection in GC B cells from Cγ1-Cre mice allowing for deletion or activation of loxP-flanked cellular genes. The implementation of PrimeFlow RNA assay for MHV-68 demonstrated the enrichment of MHV-68 in GC and isotype-switched B cells. As illustrations of virus and cellular modifications, we inserted the EBV gene LMP2A into the MHV-68 genome and induced constitutively active CD30-signaling in GC B cells through MHV-68 infections, respectively. While the LMP2A-expressing MHV-68 behaved similarly to wildtype MHV-68, virally induced constitutively active CD30-signaling in GC B cells led to the expansion of a pre-plasmablastic population. The findings underscore the potential of our novel tools to address crucial questions about the interaction between herpesviral infections and deregulated cellular gene-expression in future studies.
Species Diversity and Community Structure of Macrobenthos in the Cosmonaut Sea, East Antarctica
Authors: Mou Jianfeng , Liu Kun , Huang Yaqin , Lin Junhui , He Xuebao , Zhang Shuyi , Li Dong , Zu Yongcan , Chen Zhihua , Fu Sujing , Lin Heshan , Liu Wenhua
Published: December 2023

The Cosmonaut Sea is an under-studied area and a “white spot” for macrobenthos research. Here, we report on the species diversity and community structure of macrobenthos collected using tringle trawls on the 38th Chinese National Antarctic Research Expedition (CHINARE) in the Cosmonaut Sea, East Antarctica. A total of 11 tringle trawls were deployed at different depths across the shelf, slope and seamount of the Cosmonaut Sea. A total of 275 macrobenthic species were found from 207 to 1994 m. The species richness per station varied from 23 to 89. Echinoderms (100 species), arthropods (48 species) and mollusks (36 species) were the most dominant groups. Echinoderms and arthropods dominated in abundance at seamount stations, and echinoderms, arthropods and polychaetes dominated in abundance at slope stations, while bryozoans, corals, ascidians and sponges were abundant on the Cosmonaut Sea shelf. Depth was the major driving force influencing the distribution of macrobenthos. The main components were two core communities. One was dominated by sessile suspension feeders and associated fauna. Variants of this community include sponges and bryozoans. The other core community was dominated by mobile deposit feeders, infauna and grazers–epifauna, which included arthropods and echinoderms. The results showed that the slope (40–50° E, 65–67° S) of the Cosmonaut Sea may be an important area with complex ecological processes. The results of this study contribute to the knowledge of species diversity and communities of macrobenthos in the Cosmonaut Sea and provide monitoring data for future ecosystem health assessments and better protection.
Performance Analysis of Artificial Noise-Assisted Location-Based Beamforming in Rician Wiretap Channels
Authors: Fu Hua , Zhang Xiaoyu , Peng Linning
Published: December 2023

This paper studies the performance of location-based beamforming with the presence of artificial noise (AN). Secure transmission can be achieved using the location information of the user. However, the shape of the beam depends on the number of antennas used. When the scale of the antenna array is not sufficiently large, it becomes difficult to differentiate the performance between the legitimate user and eavesdroppers nearby. In this paper, we leverage AN to minimize the area near the user with eavesdropping risk. The impact of AN is considered for both the legitimate user and the eavesdropper. Closed-form expressions are derived for the expectations of the signal to interference plus noise ratios (SINRs) and the bit error rates. Then, a secure beamforming scheme is proposed to ensure a minimum SINR requirement for the legitimate user and minimize the SINR of the eavesdropper. Numerical results show that, even with a small number of antennas, the proposed beamforming scheme can effectively degrade the performance of eavesdroppers near the legitimate user.
Bosonic Representation of Matrices and Angular Momentum Probabilistic Representation of Cyclic States
Authors: López-Saldívar Julio A. , Man’ko Olga V. , Man’ko Margarita A. , Man’ko Vladimir I.
Published: December 2023

The Jordan–Schwinger map allows us to go from a matrix representation of any arbitrary Lie algebra to an oscillator (bosonic) representation. We show that any Lie algebra can be considered for this map by expressing the algebra generators in terms of the oscillator creation and annihilation operators acting in the Hilbert space of quantum oscillator states. Then, to describe quantum states in the probability representation of quantum oscillator states, we express their density operators in terms of conditional probability distributions (symplectic tomograms) or Husimi-like probability distributions. We illustrate this general scheme by examples of qubit states (spin-1/2 su(2)-group states) and even and odd Schrödinger cat states related to the other representation of su(2)-algebra (spin-j representation). The two-mode coherent-state superpositions associated with cyclic groups are studied, using the Jordan–Schwinger map. This map allows us to visualize and compare different properties of the mentioned states. For this, the su(2) coherent states for different angular momenta j are used to define a Husimi-like Q representation. Some properties of these states are explicitly presented for the cyclic groups C2 and C3. Also, their use in quantum information and computing is mentioned.
A Review: Remote Sensing Image Object Detection Algorithm Based on Deep Learning
Authors: Bai Chenshuai , Bai Xiaofeng , Wu Kaijun
Published: December 2023

Target detection in optical remote sensing images using deep-learning technologies has a wide range of applications in urban building detection, road extraction, crop monitoring, and forest fire monitoring, which provides strong support for environmental monitoring, urban planning, and agricultural management. This paper reviews the research progress of the YOLO series, SSD series, candidate region series, and Transformer algorithm. It summarizes the object detection algorithms based on standard improvement methods such as supervision, attention mechanism, and multi-scale. The performance of different algorithms is also compared and analyzed with the common remote sensing image data sets. Finally, future research challenges, improvement directions, and issues of concern are prospected, which provides valuable ideas for subsequent related research.
Lightweight and Secure Multi-Message Multi-Receiver Certificateless Signcryption Scheme for the Internet of Vehicles
Authors: Xu Guishuang , Yin Xinchun , Li Xincheng
Published: December 2023

The Internet of Vehicles (IoV) improves traffic efficiency and enhances driving safety through the real-time collection and analysis of traffic-related data. Numerous secure and privacy-preserving communication protocols have been proposed for the IoV. However, various security threats, privacy leakage, and inefficient communications remain unaddressed. Therefore, a lightweight and secure multi-message multi-receiver certificateless signcryption (LS-MRCLSC) scheme based on elliptic curve cryptography (ECC) is proposed. The proposed scheme guarantees secure communication and promotes messaging efficiency with multi-cast mode. Multiple key generation centers (KGCs) collaborate to generate and update the system master key (SMK) using Feldman’s verifiable secret-sharing (FVSS) algorithm, avoiding the single point of failure (SPoF) problem. Formal security proofs under the random oracle model (ROM) demonstrate that the proposed scheme meets requirements such as data confidentiality, message unforgeability, anonymity, and unlinkability. Performance evaluations confirm that the LS-MRCLSC scheme is better than similar schemes in terms of efficiency, feasibility, and scalability.
Pyrolysis of Cyclohexane and 1-Hexene at High Temperatures and Pressures—A Photoionization Mass Spectrometry Study
Authors: Tranter Robert S. , Banyon Colin , Hawtof Ryan E. , Kim Keunsoo
Published: December 2023

Cycloalkanes are important components of a wide range of fuels. However, there are few experimental data at simultaneously high temperatures and pressures similar to those found in practical systems. Such data are necessary for developing and testing chemical kinetic models. In this study, data relevant to cycloalkane pyrolysis were obtained from high repetition rate shock tube experiments coupled with synchrotron-based photoionization mass spectrometry diagnostics. The pyrolysis of cyclohexane was studied over 1270–1550 K and ~9 bar, while the more reactive primary decomposition product, 1-hexene, was studied at 1160–1470 K and ~5 bar. Insights into the decomposition of the parent molecules, the formation of primary products and the production of aromatic species were gained. Simulations were performed with models for cyclohexane and 1-hexene that were based on literature models. The results indicate that over several hundred microseconds reaction time at high pressures and temperatures the pyrolysis of cyclohexane is largely dominated by reactions initiated by cyclohexyl radicals. Furthermore, good agreement between the simulations and the experiments were observed for cyclohexane and 1-hexene with a modified version of the cyclohexane model. Conversely, the 1-hexene model did not reproduce the experimental observations.
Male-Specific Sequence in Populus simonii Provides Insights into Gender Determination of Poplar
Authors: Wang Ziyue , Lei Yijing , Liu Guanqing , Ning Yihang , Ni Runxin , Zhang Tao , Xi Mengli
Published: December 2023

The genus Populus is composed of dioecious woody plants and adult females produce large numbers of seed hairs that can affect public health and pose a potential fire risk. However, it is difficult to distinguish between males and females based on their morphology at the seedling stage. Therefore, developing a technology that identifies the gender of poplar seedlings is crucial for controlling seed hairs. In this study, we developed an approach for the early gender identification of Tacamahaca and Aigeiros species based on the male-specific sequence in Populus simonii. The gender of Tacamahaca and Aigeiros species can be accurately identified by PCR. The sequencing results showed that the male-specific sequence was conserved in P. simonii and its F1 progenies. Interestingly, there were three nucleobase differences between Tacamahaca and Aigeiros species. Sequence alignment showed that the male-specific sequence had not been assembled on the pseudochromosome. Subsequently, fluorescence in situ hybridization (FISH) was used to locate this specific sequence at the short arm end of chromosome 19 in male P. simonii. This study provides an efficient and convenient method for early gender determination of Tacamahaca and Aigeiros species and lays the groundwork for exploring key sex-determination genes.