bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Multiple Factors on the Compressive Strength of Porous Ceramsite Prepared from Secondary Aluminum Dross
Authors: Wang Yiou , Zhu Xinghan , Zhou Jinliang , Yang Jinzhong , Tian Lu , Yang Yufei
Published: November 2024

Aluminum is one of the most in-demand nonferrous metals in the world. The secondary aluminum dross (SAD) produced during aluminum smelting is a type of solid waste that urgently requires disposal. SAD, municipal solid waste incineration fly ash, and bottom slag were used as raw materials to prepare porous ceramsite in a laboratory in this study. Multi-factor design experiments were then used to explore the influence of the sintering condition on the compressive strength to provide a basis for ceramsite preparation using SAD. The results showed that, within a certain variation range, the levels of each factor showed overall positive correlations with the ceramsite compressive strength. The contributions of the ceramsite particle size, the silicon–aluminum ratio (Si/Al), the sintering temperature, and the sintering time to the compressive strength of the porous ceramsite then decreased. The factors had a synergistic effect. The interactive effect of multiple factors on the porous ceramsite compressive strength rose with an increase in the particle size and Si/Al ratio. The average compressive strength of the porous ceramsite prepared in this study was 4.06 ± 3.71 MPa, and the maximum compressive strength was 14.13 MPa. The highest ceramsite compressive strength was achieved under a sintering temperature of 1270 °C, a particle size of 2 cm, a sintering time of 30 min, and a silicon–aluminum ratio of 1.5. In addition, there was a reaction relationship between the multiple factors involved in the sintering of the SAD-based porous ceramsite. Pilot or industrial tests should be conducted in the future based on these experiments and the intended ceramsite use.
Evaluating the Impact of Washing Conditions on the Color Changes of Naturally Colored Cotton Fabrics: A Focus on Detergents, Water Types, and Temperature
Authors: Aliei Hesam , Carrera-Gallissa Enric , Cayuela Diana
Published: November 2024

Intrinsically colored cotton is crucial for sustainability as it eliminates the need for chemical dyes, reducing water pollution and carbon footprint. It also preserves biodiversity by using fewer pesticides and supports eco-friendly, ethical product creation. This research aims to examine the factors that influence the color change that occurs in naturally colored organic cotton (NaCOC) fabrics when washed under normal household conditions, and it focuses on special detergents designed for people with skin hypersensitivity. The study observes the impact of various washing conditions on the color changes of the fabrics. Specifically, three specific detergents, two types of water (tap and distilled), and three different temperatures (20, 40, and 60 °C) are taken into consideration as variables. By using colorimetric measures and correlating the results with the significant variables of the experimental design, the study evaluates how washing practices affect both the color and the overall integrity of the fabric. The findings demonstrate that the water hardness is the most influential variable when it comes to the color changes in the fabrics. Additionally, higher washing temperatures exacerbate color changes, particularly in hard water conditions. These results provide valuable insights for maintaining the color integrity of NaCOC fabrics during washing.
Research on Unbalanced Vibration Characteristics and Assembly Phase Angle Probability Distribution of Dual-Rotor System
Authors: Li Hui , Shi Changzhi , Li Xuejun , Li Mingfeng , Bian Jie
Published: November 2024

This paper addresses the complex issue of vibration response characteristics resulting from the unbalanced assembly of the double rotors in the 31F aero-engine. The study investigates the vibration response behavior of the dual-rotor system through the adjustment of rotor assembly phase angle. Initially, a dynamic model of the four-disk, five-pivot dual-rotor system is established, with its natural frequencies and vibration modes verified. The influence of size and the position of the unbalance on the vibration amplitude in the dual-rotor system is analyzed. Additionally, the probability distribution of the assembly phase angles for both the compressor and turbine sections of the low-pressure rotor is examined. The results indicate that for the low-pressure rotor exhibiting excessive vibration, adjusting the assembly phase angle of the rotors’ system’s compressor or the turbine section by 180 degrees leads to a vibration qualification rate of 70.1435%. This finding is consistent with the observations from the field experience method used in the former Soviet Union. Finally, corresponding experimental verification is conducted.
A Soft Start Method for Doubly Fed Induction Machines Based on Synchronization with the Power System at Standstill Conditions
Authors: Guerrero José M. , Mahtani Kumar , Aranzabal Itxaso , Gómez-Cornejo Julen , Sánchez José A. , Platero Carlos A.
Published: November 2024

Due to their exceptional operational versatility, doubly fed induction machines (DFIM) are widely employed in power systems comprising variable renewable energy-based electrical generation sources, such as wind farms and pumped-storage hydropower plants. However, their starting and grid synchronization methods require numerous maneuvers or additional components, making the process challenging. In this paper, a soft start method for DFIM, inspired by the traditional synchronization method of synchronous machines, is proposed. This method involves matching the frequencies, voltages, and phase angles on both sides of the main circuit breaker, by adjusting the excitation through the controlled power converter at standstill conditions. Once synchronization is achieved, the frequency is gradually reduced to the rated operational levels. This straightforward starting method effectively suppresses large inrush currents and voltage sags. The proposed method has been validated through computer simulations and experimental tests, yielding satisfactory results.
CFD Study and Regression Analysis of the MHD Mixed Convection of CNT-Water Nanofluid in a Vented Rounded Edge Rectangular Cavity Having Inner Vertical Rod Bundle
Authors: Aich Walid , Hilali-Jaghdam Inès , Alshahrani Amnah , Maatki Chemseddine , Alshammari Badr M. , Kolsi Lioua
Published: November 2024

This current work provides a comprehensive Computational Fluid Dynamics (CFD) investigation of three-dimensional magnetohydrodynamic (MHD) mixed convection of carbon nanotube (CNT)-water nanofluid within a vented rectangular cavity featuring an internal vertical rod bundle with circular, square, and triangular cross-sections. The finite element method (FEM) was used to investigate the effects of key parameters, including the Richardson number (0.01 ≤ Ri ≤ 10), Hartmann number (0 ≤ Ha ≤ 100), and CNT nanoparticle concentration (0 ≤ ϕ ≤ 0.045), in relation to fluid flow and heat transfer performance. The CNT nanoparticle incorporation increases the nanofluid’s heat transfer capacity by up to 22%, with the highest average Nusselt number (Nuav) achieved with circular rods at ϕ = 0.045, which corresponds to the higher convective heat transfer efficiency. The magnetic field further stabilizes the flow by reducing thermal convection irregularities, with a 15% improvement in temperature distribution uniformity when Ha = 100. The investigation’s outcomes reveal that due to their smoother geometries, the circular rods exhibit better thermal exchange rates compared to square and triangular rods. Moreover, a polynomial regression model is used to correlate the governing parameters and heat transfer rates, and it achieves a high R2 of 0.964. These findings highlight the potential of CNT-water nanofluid and magnetic field applications for thermal management optimization in various engineering systems.
Directed Knowledge Graph Embedding Using a Hybrid Architecture of Spatial and Spectral GNNs
Authors: Hou Guoqiang , Yu Qiwen , Chen Fan , Chen Guang
Published: November 2024

Knowledge graph embedding has been identified as an effective method for node-level classification tasks in directed graphs, the objective of which is to ensure that nodes of different categories are embedded as far apart as possible in the feature space. The directed graph is a general representation of unstructured knowledge graphs. However, existing methods lack the ability to simultaneously approximate high-order filters and globally pay attention to the task-related connectivity between distant nodes for directed graphs. To address this limitation, a directed spectral graph transformer (DSGT), a hybrid architecture model, is constructed by integrating the graph transformer and directed spectral graph convolution networks. The graph transformer leverages multi-head attention mechanisms to capture the global connectivity of the feature graph from different perspectives in the spatial domain, which bridges the gap between frequency responses and, further, naturally couples the graph transformer and directed graph convolutional neural networks (GCNs). In addition to the inherent hard inductive bias of DSGT, we introduce directed node positional and structure-aware edge embedding to provide topological prior knowledge. Extensive experiments demonstrate that the DSGT exhibits state-of-the-art (SOTA) or competitive node-level representation capabilities across datasets of varying attributes and scales. Furthermore, the experimental results indicate that the homophily and degree of correlation of the nodes significantly influence the classification performance of the model. This finding opens significant avenues for future research.
Applying the Theory of Multi-Operations to Building Decision-Making Systems with a Large Number of Uncertainties
Authors: Todikov Sergey , Shichkina Yulia , Peryazev Nikolay
Published: November 2024

This article is devoted to the practical application of multi-operations theory to the construction of decision-making systems and the description of the subsequent research results. Unlike classical multi-valued and fuzzy logic, where events are described by only two logical values, namely, “true” and “false”, when there are various types of uncertainty between these two states, the theory of multi-operations can be used to describe events using a larger number of logical values for the uncertainties between these states. This article demonstrates a new approach to processing input information using rank 3 multi-operations, i.e., considering input information for a set of three logical values and five values of uncertainty. This approach allows for saving time and resources when forming a subject area model for decision-making systems and when working with specific users. The application of this approach is illustrated in the article by using the example of determining the area of human disease. When testing this system, which is built on the basis of rank 3 multi-operations, we show that applying multi-operations theory allows for significant expansion of the range of accepted decisions; this makes the system more flexible for the construction of human–machine interfaces and organizes the integration of efforts in the development of humans and machines with a common goal.
Equilibrium Strategies in an Mn/M/1 Queue with Server Breakdowns and Delayed Repairs
Authors: Pan Yingying , Zhang Jingchuan , Liu Zaiming
Published: November 2024

Ophthalmic units use sophisticated equipment to enable accurate diagnosis of refractive errors. This equipment is subject to two types of breakdowns. One is simple breakdown which can be repaired in-house, and the other is complex breakdown which requires repair by the original equipment manufacturer (OEM) and results in a delay time between the server breakdown and the start of the repair. In this paper, we model this scenario as an Mn/M/1 queuing system with two types of breakdowns and delayed repairs due to complex breakdowns, where the delay time and repair times for simple and complex breakdowns are generally distributed. We obtain the steady-state probabilities and provide the recursive formulas for the Laplace–Stieltjes transforms (LSTs) of conditional residual delay time and repair times given the system state. For the fully observable case, we derive the equilibrium joining strategies of customers who decide to join or balk based on their observation of the system state. Moreover, two numerical experiments are conducted to explore the equilibrium joining probabilities.
Therapeutic Potential of Oligo-Fucoidan in Mitigating Peritoneal Dialysis-Associated Fibrosis
Authors: Chen Yu-Wei , Wu Mei-Yi , Huang Nai-Jen , Wu Mai-Szu , Hsu Yung-Ho , Liao Chia-Te , Chen Cheng-Hsien
Published: November 2024

Peritoneal dialysis (PD) serves as a home-based kidney replacement therapy with increasing utilization across the globe. However, long-term use of high-glucose-based PD solution incites repeated peritoneal injury and inevitable peritoneal fibrosis, thus compromising treatment efficacy and resulting in ultrafiltration failure eventually. In the present study, we utilized human mesothelial MeT-5A cells for the in vitro experiments and a PD mouse model for in vivo validation to study the pathophysiological mechanisms underneath PD-associated peritoneal fibrosis. High-glucose PD solution (Dianeal 4.25%, Baxter) increased protein expression of mesothelial–mesenchymal transition (MMT) markers, such as N-cadherin and α-SMA in MeT-5A cells, whereas it decreased catalase expression and stimulated the production of reactive oxygen species (ROS). Furthermore, macrophage influx and increased serum pro-inflammatory cytokines, such as IL-1β, MCP-1, and TNF-α, were observed in the PD mouse model. Interestingly, we discovered that oligo-fucoidan, an oligosaccharide extract from brown seaweed, successfully prevented PD-associated peritoneal thickening and fibrosis through antioxidant effect, downregulation of MMT markers, and attenuation of peritoneal and systemic inflammation. Hence, oligo-fucoidan has the potential to be developed into a novel preventive strategy for PD-associated peritoneal fibrosis.
Endophytic Fungi Co-Culture: An Alternative Source of Antimicrobial Substances
Authors: Tironi Lucas Silva , Carletto Lucilene Bento , Silva Eliane Oliveira , Schripsema Jan , Luiz Jaine Honorata Hortolan
Published: November 2024

Antimicrobial resistance is becoming a critical issue due to the widespread and indiscriminate use of antibiotics and antifungals to treat common infections, leading to a growing shortage of effective drugs. Moreover, the increase in antimicrobial resistance is enhancing the pathogenicity and virulence of various pathogens. Microorganisms are key sources of chemically diverse specialized metabolites, which are produced in the final stages of their growth cycle. These metabolites hold significant value in chemical, pharmaceutical, and agrochemical industries. One of the major challenges researchers face in this field is the frequent isolation of already-known substances when classical protocols are used. To address this, several innovative strategies have been developed. The co-culture approach is a powerful tool for activating silent biosynthetic gene clusters, as it simulates natural microbial environments by creating artificial microbial communities. This method has shown promising results, with new compounds being isolated and the yields of target substances being improved. In this context, this review provides examples of antimicrobial compounds obtained from co-cultures of endophytic fungi, conducted in both liquid and solid media. Additionally, the review discusses the advantages and challenges of the co-culture technique. Significance and Impact of the Study: Microbial co-culture is a valuable strategy for discovering new natural products with antimicrobial activity, as well as for scaling up the production of target substances. This review aims to summarize important examples of endophyte co-cultures and highlights the potential of endophytic fungi co-culture for pharmacological applications.
Heterologous Expression of the Antiviral Lectin Griffithsin in Probiotic Saccharomyces boulardii and In Vitro Characterization of Its Properties
Authors: Tang Jie , Li Ran , Jiang Tingyu , Lv Jiachen , Jiang Yuwei , Zhou Xingjian , Chen Hong , Li Meiliang , Wu Aimin , Yu Bing , Takala Timo M. , Saris Per E. J. , Li Shuhong , Fang Zhengfeng
Published: November 2024

In this study, the probiotic yeast Saccharomyces boulardii was engineered to secrete the antiviral lectin griffithsin. Twelve genetic tools with the griffithsin gene were cloned into the vector pSF-TEF1-URA3 and introduced into S. boulardii. In the recombinant strains, a 16.9 kDa band was detected using SDS-PAGE and further recognized by griffithsin antibody with Western blotting. S. boulardii strains FM, FT, HC, and HE with a high yield of griffithsin were acquired for property characterization in vitro. The four recombinant strains displayed a similar growth pattern to that of the control strains, while their morphological characteristics had changed according to scanning electron microscopy. In simulated gastrointestinal digestive fluids, the survival rates of S. boulardii FM, FT, and HC were significantly decreased (86.32 ± 1.49% to 95.36 ± 1.94%) compared with those of the control strains, with survival rates between 95.88 ± 0.00% and 98.74 ± 1.97%. The hydrophobicity of S. boulardii FM, the strain with the highest griffithsin production, was significantly increased to 21.89 ± 1.07%, and it exhibited a reduced auto-aggregation rate (57.64 ± 2.61%). Finally, Vero cells infected with porcine epidemic diarrhea virus (PEDV) were used to evaluate the strains’ antiviral activity, and the rate at which S. boulardii FM inhibited PEDV reached 131.36 ± 1.06%, which was significantly higher than that of the control group.
Recent Catalytic Applications of Ferrocene and Ferrocenium Cations in the Syntheses of Organic Compounds
Authors: Bauer Eike B.
Published: November 2024

Ferrocene and its oxidized counterpart, the ferrocenium cation, represent a fascinating class of organometallic compounds with broad utility across various fields, including organic synthesis, pharmaceuticals, and materials science. Over the years, ferrocene, ferrocenium cations, and their derivatives have also gained prominence for their versatility in catalytic processes. This review article offers an overview of the research of the last decade into ferrocene- and ferrocenium-based catalysis. Key developments are highlighted in catalytic oxidation, cross-coupling, polymerization reactions, and redox-switchable catalysis, as well as the application of ferrocenium cations as Lewis acid catalysts.
Rapid Degradation of Bisphenol F Using Magnetically Separable Bimetallic Biochar Composite Activated by Peroxymonosulfate
Authors: Liang Hui , Li Ruijuan , Liu Tongjin , Li Rumei , Zhu Yuxiao , Fang Feng
Published: November 2024

Peroxymonosulfate (PMS)-based advanced oxidation processes have shown potential for the removal of organic contaminants; however, the preparation of catalysts with high degradation efficiencies and rapid reaction rates remains a challenge. In this study, we have successfully synthesized CoFe bimetallic modified corn cob-derived biochar (CoFe/BC) for the activation of PMS, achieving the rapid and efficient degradation of bisphenol F (BPF). The synthesized CoFe/BC catalyst demonstrated excellent catalytic performance, achieving over 99% removal within 3 min and exhibiting a removal rate of 90.0% after five cycles. This could be attributed to the cyclic transformation of Co and Fe, which sustained rapid PMS activation for BPF degradation, and Co0/Fe0 played a significant role in the cyclic transformation. Furthermore, the electron paramagnetic resonance tests confirmed that •SO4− and •OH were the primary reactive oxygen species, while •O2− played a minor role in BPF degradation. This study highlights the high degradation efficiency, rapid reaction rate, excellent magnetic separation properties, and exceptional reusability of CoFe/BC catalysts for BPF removal, providing valuable insights for practical wastewater treatment.
Visible Light Photoredox Catalysis in the Synthesis of Phosphonate-Substituted 1,10-Phenanthrolines
Authors: Morozkov Gleb V. , Troickiy Artem A. , Averin Alexei D. , Mitrofanov Alexander Yu. , Abel Anton S. , Beletskaya Irina P.
Published: November 2024

Photoredox-catalyzed phosphonylation of bromo-substituted 1,10-phenanthrolines under visible light irradiation was studied. The reaction was shown to proceed under mild conditions with Eosin Y as a photocatalyst in DMSO under blue light irradiation. It is transition-metal-free and affords the target phosphonate-substituted 1,10-phenanthrolines in moderate yields (26–51%) in 22 to 40 h. The rate and selectivity of the reaction depend largely on the position of the bromine atom, as well as on the nature and position of other substituents in the 1,10-phenanthroline core.
Aqueous-Phase Reforming of Biogas Slurry over MOF-Derived α-MoO3 Catalyst for Producing Renewable Hydrogen: Effect of Fermenting Time
Authors: Bu Qingguo , Wang Jian , Chen Yuxuan , Tao Junyu , Kumar Akash , Yan Beibei , Chen Guanyi
Published: November 2024

Aqueous-phase reforming (APR) is an alternative method for treating and utilizing biogas slurry (BS) to produce renewable hydrogen from organic oxygen-containing wastewater. Considering the fluctuating characteristics of BS with changes in the degree of fermentation, developing an efficient catalyst is a major concern for the APR of BS. The novel catalyst based on molybdenum-based metal–organic-framework-derived oxides (Mo-MOF-derived α-MoO3) was reported in this study. The results indicated that the variables (e.g., pH, organic load, and salinity) of BS corresponded to the fermentation times and exhibited decreasing trends after APR under the reaction conditions of 225 °C and 30 min. Decarboxylation was identified as the main side reaction in the APR of BS over the catalyst. An optimal yield of 2.17 mLhydrogen/mLBS was achieved when BS was obtained from 6 days of fermentation. Finally, the Mo-MOF-derived α-MoO3 catalyst was obtained from the greater specific surface area of MOFs. The catalyst had a weaker acidity than the initial α-MoO3, making it more preferred for facilitating the APR of BS.
DABCO-Intercalated α-Zirconium Phosphate as a Latent Thermal Catalyst in the Reaction of Urethane Synthesis
Authors: Shimomura Osamu , Arisaka Yushi , Rahmawati Astrid , Kabir Shekh Md. Mamun , Shizuma Motohiro , Ohtaka Atsushi
Published: November 2024

The mixture of hexamethylene diisocyanate (HDI) and butanol (BuOH) with the intercalation compound of 1,4-diazabicyclo[2.2.2]octane (DABCO) with α-zirconium phosphate (α-ZrP) has been evaluated as a latent thermal catalyst at varying temperatures. α-ZrP·DABCO did not show activity at 25 °C, but showed a high level of activity at a higher temperature of 80 °C. To clarify the reaction behavior of HDI-BuOH with α-ZrP·DABCO, a viscosity value of 1200 mPa·s·g/cm2 was reached at 80 °C for 30 min. To investigate the deintercalation behavior of DABCO from the α-ZrP interlayer, it was investigated in BuOH and in HDI, respectively, under heated conditions. Interestingly, XRD patterns showed a reduction in α-ZrP·DABCO for the interlayer distance due to the deintercalation of DABCO in BuOH, while no changes associated with the deintercalation of DABCO were observed in HDI. Butanol was found to be important for the deintercalation of DABCO. To examine the reactivity of bifunctional monomers, the reaction of 1,4-butanediol (1,4-BDO) and HDI with α-ZrP·DABCO were investigated to show good reactivity at 80 °C and stability at 40 °C.
Intradialytic Parenteral Nutrition in Patients on Hemodialysis: A Multicenter Retrospective Study
Authors: Arias-Guillén Marta , González Juan Carlos , Betancourt Loreley , Coll Elisabeth , Collado Silvia , Romano-Andrioni Bárbara , Lupiañez-Barbero Ascensión , Garro Julia , Duarte Verónica , Soler-Majoral Jordi , Calabia Jordi
Published: November 2024

Background and Objective: To evaluate the effectiveness and safety of intradialytic parenteral nutrition (IDPN) on different nutritional outcomes. Methods: This was a retrospective analysis for a “routinely collected data bank” in a multicenter cohort, conducted on consecutive malnourished or at-risk of malnutrition patients with chronic kidney disease on hemodialysis who underwent IDPN with a three-in-one parenteral nutrition formula for a period ≥ 2 weeks. The primary endpoint was the mean change in the malnutrition inflammation score (MIS) score between baseline and the last follow-up visit on IDPN. Results: Fifty-six patients were included. The mean age was 72.4 ± 12.0 years, and 24 (42.9%) were women. In the overall study sample, MIS significantly decreased from 16.4 (95%CI: 15.3–17.65) at baseline to 14.3 (95%CI: 12.8–15.8) at the last follow-up visit on IDPN (p = 0.0019). Fifteen (26.8%) patients achieved a MIS reduction ≥ 5 points after IDPN. As compared to baseline, IDPN significantly reduced the proportion of patients with protein-energy wasting (PEW) (89.3% versus 66.1%, respectively, p = 0.0023). Regarding analytical parameters, serum albumin (p = 0.0003) and total proteins (p = 0.0024) significantly increased after IDPN administration. Throughout the study’s follow-up period, 45 (80.4%) patients reported experiencing some type of adverse event. Conclusions: IDPN was associated with a significant improvement in the nutritional profile. Notably, our research found that the administration of IDPN over a duration > 3 months significantly improved the nutritional status of patients evaluated by the MIS test.
Coffee Intake in Brazil Influences the Consumption of Sugar, Sweets, and Beverages
Authors: Simões Marijoe Braga Alves , Brandão Joana Maia , Antunes Anna Beatriz Souza , Sichieri Rosely
Published: November 2024

Background/Objectives: Considering the high consumption of coffee in Brazil, this study aimed to investigate the relationship between coffee consumption and the intake of added sugar, non-caloric sweeteners, sugary beverages, and foods. Methods: A modified case-crossover study was conducted using data from the national “Household Budget Survey (POF)” which 38,854 participants. Dietary intake was assessed using 24-h recalls on two non-consecutive days. Days with and without coffee consumption were compared (case-crossover) to evaluating the effects on sugar, sweeteners, sugary foods, sugar-sweetened beverages (SSB), and milk. Results: 87% of Brazilians aged 10 and older consumed coffee on a giving day. The consumption of all food groups decreased as days of coffee consumption increased, except for non-caloric sweeteners and sugar, which increased. In the case-crossover analysis (2192 men and 2580 women), women who consumed coffee on one of the two days showed an increase of 10 g of sugar and of 0.10 mL (2 drops) of sweeteners. For men values were 8 g and 0.05 mL. Also, women reduced sugar-sweetened beverages (−56.8 mL/day), while men reduced milk intake (−25.9 mL/day). Conclusions: Coffee consumption was associated with increased intake of sugar and non-caloric sweeteners and lower intake of sweets, SSB, and milk. Reducing sweets and SSB is beneficial but increasing sweeteners and reducing milk are not. Potential strategies include encouraging the use of milk in coffee instead of sugar and sweeteners, as well as reducing the size of sugar sachets, which in Brazil typically range from 5 g to 8 g.
Dietary Docosahexaenoic Acid-Rich Supplementation Decreases Neurotoxic Lipid Mediators in Participants with Type 2 Diabetes and Neuropathic Pain
Authors: Durán Alfonso M. , Zamora Francis , De León Marino
Published: November 2024

Background/Objectives: There is increasing evidence linking circulating neurotoxic lipids to the progression of chronic neuroinflammatory diseases in the peripheral and central nervous systems. Strategies to modify lipid profiles, such as docosahexaenoic acid (DHA)-rich supplementation, may aid in managing conditions like painful diabetic neuropathy (pDN). In a previous study, we demonstrated that three months of DHA supplementation significantly altered the metabolomic profile of patients with painful diabetic neuropathy (pDN), resulting in symptom improvement. This study investigates whether DHA-rich supplementation reduces neurotoxic lipid mediators associated with pDN in individuals with type 2 diabetes mellitus (T2DM). Methods: Forty individuals with type 2 diabetes participated in the “En Balance-PLUS” study, attending weekly lifestyle and nutrition education sessions while receiving daily supplementation of 1000 mg DHA and 200 mg EPA. Pain levels were assessed using the Short-Form McGill Pain Questionnaire (SF-MPQ) at baseline and after three months. Blood serum samples collected at these time points underwent untargeted lipidomic analyses, with ELISA used to evaluate biomarkers of necrosis (MLKL), autophagy (ATG5), and lipid chaperone protein (FABP5). Results: Untargeted lipidomic analysis revealed that several neurotoxic-associated lipids significantly decreased after DHA-rich supplementation. Also, circulating levels of MLKL were reduced, while protein levels of ATG5 and FABP5 significantly increased. Conclusions: The reduction of circulating neurotoxic lipids and increase in neuroprotective lipids following DHA-rich supplementation are consistent with the reported roles of omega-3 polyunsaturated fatty acids (PUFAs) in reducing adverse symptoms associated with neuroinflammatory diseases and painful neuropathy.
Impact of Adlay-Based Formula on Pain and Discomfort in Women with Dysmenorrhea: A Randomized Controlled Trial
Authors: Chiang Yi-Fen , Huang Ko-Chieh , Ali Mohamed , Hsia Shih-Min
Published: November 2024

Background: Primary dysmenorrhea, a highly prevalent condition that significantly impacts women’s daily activities and quality of life, occurs without underlying pelvic pathological changes. Conventional treatments, such as warm water therapy, provide temporary relief; however, more effective interventions are needed. This study aimed to evaluate the effectiveness of an Adlay-based formula in reducing dysmenorrhea symptoms through randomized controlled trials. Methods: A total of 69 participants were randomly assigned to either the Adlay-based formula group (n = 35) or the placebo group (n = 34). Baseline characteristics, including age, age of menarche, dysmenorrhea onset, menstrual duration, BMI, blood pressure, and heart rate, were comparable between groups. The primary outcomes were measured using the Visual Analogue Scale (VAS) for dysmenorrhea, pain assessment scales, the Menstrual Distress Questionnaire (MDQ), and serum levels of inflammatory biomarkers (PGE2, PGF2α, IL-6, Hs-CRP). Results: The intervention group showed a significant reduction in VAS scores at both the first treatment and at the end of the study compared to baseline and the placebo group. Pain assessments indicated improvements in persistent pain, dull pain, exhaustion, nausea/vomiting, lower abdominal swelling, back pain, diarrhea, and cold sweats. Additionally, biomarker analysis revealed significant reductions in PGE2, PGF2α, and Hs-CRP levels in the intervention group, with no significant change in IL-6 levels. Conclusions: The Adlay-based formula effectively alleviated dysmenorrhea symptoms, improved pain and discomfort, and reduced inflammatory biomarkers compared to placebo. These findings suggested that the formula could serve as a promising alternative for managing primary dysmenorrhea.
Translation and Validation of the Greek Food Allergy Quality of Life Questionnaire—Parent Form
Authors: Vassilopoulou Emilia , Comotti Anna , Athanasaki Dafni , Milani Gregorio Paolo , Agostoni Carlo , Konstantinou George N.
Published: November 2024

Background: The prevalence of food allergy is increasing, posing a significant health concern. Assessing health-related quality of life (HRQOL) in individuals with food allergies is crucial, and various questionnaires exist for this purpose. However, translation and validation of these tools are necessary to ensure cultural relevance. This study aimed to translate the FAQLQ-PF into Greek and perform a cross-sectional validation to assess its effectiveness in evaluating HRQOL among Greek children with food allergies. Methods: Parents of children aged 0 to 12 years diagnosed with food allergy completed the Greek version of the FAQLQ-PF, consisting of 30 items across three subscales. Socio-demographic and clinical data were collected. Statistical analyses included nonparametric tests, correlation analysis for validity, and Cronbach’s alpha for internal consistency. Results: Out of 85 participants, 81 were included. The FAQLQ-PF demonstrated excellent internal consistency (Cronbach’s α = 0.94). Validity evaluation indicated its ability to measure HRQOL in younger children. HRQOL in the Greek pediatric population was significantly correlated with the number of food allergies, symptoms, parental and child concerns, anxiety levels, and activity restrictions. However, sex and general health status were not significantly correlated with HRQOL. Conclusions: The Greek translation and validation of FAQLQ-PF provides insights into HRQOL among Greek children with food allergies. Factors such as anaphylactic reactions, epinephrine autoinjector usage, number of food allergies, and symptoms influenced HRQOL in this population.
Anti-Inflammatory Effects of Aptamin C in Pulmonary Fibrosis Induced by Bleomycin
Authors: Shin Seulgi , Jo Hyejung , Agura Tomoyo , Jeong Seoyoun , Ahn Hyovin , Pang Soyoung , Lee June , Park Jeong-Ho , Kim Yejin , Kang Jae Seung
Published: November 2024

Background/Objectives: Vitamin C is a well-known antioxidant with antiviral, anticancer, and anti-inflammatory properties. However, its therapeutic applications are limited by rapid oxidation due to heat and light sensitivity. Aptamin C, which employs aptamers to bind vitamin C, has demonstrated enhanced stability and efficacy. This study investigates the potential of Aptamin C to inhibit the progression of pulmonary fibrosis, a prominent inflammatory lung disease with no effective treatment. Methods: Mice bearing bleomycin-induced pulmonary fibrosis were administered vitamin C or Aptamin C, and their weight changes and survival rates were monitored. Inflammatory cell infiltration was assessed in the bronchoalveolar lavage fluid (BALF), and the degree of alveolar fibrosis was measured by H&E and Masson’s trichrome staining. To elucidate the mechanism of action of Aptamin C, Western blot analysis was performed in HaCaT and lung tissues from bleomycin-induced pulmonary fibrosis mice. Results: The Aptamin C-treated group showed a notably higher survival rate at 50%, whereas all subjects in the vitamin C-treated group died. Histological examination of lung tissue showed that inflammation was significantly suppressed in the Aptamin C-supplemented group compared to the vitamin C-supplemented group, with a 10% greater reduction in cell infiltrations, along with noticeably less tissue damage. Additionally, it was observed that Aptamin C increased SVCT-1 expression in the HaCaT cells and the lung tissues. Conclusions: Taken together, Aptamin C not only increases the stability of vitamin C but also induces an increase in SVCT-1 expression, facilitating greater vitamin C absorption into cells and tissues, thereby inhibiting the progression of symptoms and associated inflammatory responses in pulmonary fibrosis.
Study of Cytotoxicity of Spiro-Fused [3-Azabicyclo[3.1.0]hexane]oxindoles and Cyclopropa[a]pyrrolizidine-oxindoles Against Tumor Cell Lines
Authors: Kornev Anton A. , Shmakov Stanislav V. , Ponyaev Alexander I. , Stepakov Alexander V. , Boitsov Vitali M.
Published: November 2024

Background: A series of spiro-fused heterocyclic compounds containing cyclopropa[a]pyrrolizidine-2,3′-oxindole and 3-spiro[3-azabicyclo[3.1.0]-hexane]oxindole frameworks were synthesized and studied for their in vitro antiproliferative activity against human erythroleukemia (K562), cervical carcinoma (HeLa), acute T cell leukemia (Jurkat), melanoma (Sk-mel-2) and breast cancer (MCF-7) as well as mouse colon carcinoma (CT26) cell lines. Methods: Cell proliferation was evaluated in vitro by MTS assay. Confocal microscopy was used to study actin cytoskeleton structure and cell motility. Cell cycle analysis was evaluated by flow cytometry. Results: It was found that compounds 4, 8, 18 and 24 showed antiproliferative activity against the Jurkat, K-562, HeLa and Sk-mel-2 cell lines with IC50 ranging from 2 to 10 μM (72 h). Evaluation of the impact on cell cycle progression showed that the tested compounds achieved significant cell-cycle perturbation with a higher accumulation of cells in the SubG1 and G0/G1 phases of the cell cycle, in comparison to the negative control. I Incubation with tested compounds led to the disappearance of stress fibers (granular actin was distributed diffusely in the cytoplasm in up to 38% of treated HeLa cells) and changes in the number of filopodia-like deformations (reduced from 93% in control cells to 64% after treatment). The impact on the Sk-mel-2 cell actin cytoskeleton structure was even greater: granular actin was distributed diffusely in the cytoplasm in up to 90% of treated cells while the number of filopodia-like deformations was reduced by up to 23%. A scratch test performed on the human melanoma cell line showed that these cells did not fill the scratched strip and lose their ability to move under treatment. Conclusions: The obtained results support the antitumor effect of the tested spiro-compounds and encourage the extension of this study in order to improve their anticancer activity as well as reduce their toxicological risks.
Berbamine Promotes the Repair of Lower Limb Muscle Damage in Chronic Limb-Threatening Ischemia by Inhibiting Local Inflammation and NF-κB Nuclear Translocation
Authors: Zheng Lei , Zhao Biao , Zhang Zhenxi , Liu Yutong , Zhang Yingying , Cai Jing , Qiao Tong
Published: November 2024

Background/Objectives: Chronic Limb-Threatening Ischemia (CLTI) is a chronic limb ischemic disease caused by vascular lesions, characterized by pain, ulcers, and gangrene, which can be life-threatening in severe cases. The objective of this study is to explore whether Berbamine (BBM) can protect against and repair ischemic muscle tissue in the lower limbs; Methods: Using a mouse hindlimb ischemia (HLI) model, 36 C57BL6 mice were divided into sham, HLI, and HLI+BBM treatment groups. Results: Our findings indicate that BBM can restore motor function and muscle tissue pathology in mice, potentially by inhibiting the nuclear translocation of nuclear factor kappa-B (NF-κB), thereby alleviating tissue inflammation caused by chronic ischemia, reducing muscle cell apoptosis, inhibiting M1 macrophage polarization, and promoting angiogenesis. Conclusions: Our research suggests that BBM has the potential to protect against ischemic damage in lower limb muscle tissue, providing a new approach to the treatment of CLTI.
Danaparoid—Consensus Recommendations on Its Clinical Use
Authors: Bauersachs Rupert M. , Lindhoff-Last Edelgard , Klamroth Robert , Koster Andreas , Schindewolf Marc , Magnani Harry
Published: November 2024

(1) Background: Danaparoid sodium is a heparinoid antithrombotic that has been used for over 40 years for prophylaxis of DVT in non-HIT patients and for the treatment of heparin-induced thrombocytopenia (HIT) with and without thrombosis. This update summarises current information on its pharmacology and reviews danaparoid dose management in a broad spectrum of clinical situations, including off-label indications. (2) Methods: Evidence from published clinical studies, case reports, compassionate use of danaparoid, and spontaneously reported serious adverse events is summarised and analysed by an interdisciplinary expert group to develop a consensus on dosing regimens of danaparoid for complex clinical situations, including vulnerable patient populations. (3) Results: Dosing regimens are proposed, together with monitoring recommendations and target anti-factor Xa ranges. (4) Conclusion: In a comprehensive summary detailed interdisciplinary dosing recommendations are described to provide a basis for safe and effective use of danaparoid.
Formulating a Horseradish Extract in Phospholipid Vesicles to Target the Skin
Authors: De Luca Maria , Casula Luca , Tuberoso Carlo Ignazio Giovanni , Pons Ramon , Morán Maria del Carmen , García María Teresa , Martelli Giuseppe , Vassallo Antonio , Caddeo Carla
Published: November 2024

Background/Objectives: Horseradish (Armoracia rusticana L.) roots—largely used in traditional medicine for their multiple therapeutic effects—are a rich source of health-promoting phytochemicals. However, their efficacy can be compromised by low chemical stability and poor bioavailability. Incorporation into phospholipid vesicles is often proposed to tackle this problem. Methods: In this study, a hydroalcoholic extract was produced from horseradish roots. The extract was characterized by UPLC-MS and HPLC-PDA and formulated in conventional liposomes and Penetration Enhancer-containing Vesicles (PEVs) for skin application. Results: The obtained nanovesicles were small in size (<100 nm), negatively charged, uni/bilamellar, and with high values of entrapment efficiency (>85%) for the flavonoids identified in the extract. Both the free and the nanoformulated extract showed optimal biocompatibility, measured as the absence of hemolysis of erythrocytes and absence of cytotoxicity in skin cell lines. Furthermore, the nanoformulations displayed antioxidant activity in vitro. Conclusions: The proposed nananoformulations could be exploited to counteract oxidative stress involved in the pathogenesis and progression of numerous skin disorders.
Bioactive Silk Revolution: Harnessing Curcuminoid Dye and Chitosan for Superior Antimicrobial Defence and UV Shielding
Authors: Do Khai Ly , Ahsan Taswar , Wahab Abdul , Tayyab Muhammad , Yin Xinqi , Pan Nengjie , Huang Tao , Mushtaq Asim , Su Miao
Published: November 2024

Background/Objectives: The use of natural colourants is gaining attention due to their biocompatibility and functional benefits. This study introduces a different approach using turmeric (Curcuma longa L.) dye extract combined with chitosan to significantly enhance the antibacterial and UV-shielding properties of silk. Methods: The turmeric dye’s chemical composition was analyzed using liquid chromatography mass spectrometry (LC-MS), UV–visible spectroscopy, and Fourier-transform infrared spectroscopy (FTIR). The dyed silk’s colourfastness was tested through rubbing, washing, and light exposure. Results: The chitosan-mordanted silk showed strong antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), as well as antifungal activity against Aspergillus niger (A. niger). It also demonstrated a high ultraviolet protection factor (UPF). For comparison, alum-mordant was used, and chitosan proved more effective. Beyond its use as a dye, turmeric is renowned for its medicinal properties. Its antioxidant, anticancer, and anti-inflammatory properties have been extensively researched, which are primarily linked to its curcuminoid compounds. Turmeric is used in traditional medication to treat digestive issues, arthritis, and skin diseases. Conclusions: This work underscores the innovative use of plant-based dye extracts and natural mordants like chitosan as a sustainable alternative to conventional metallic mordants, paving the way for the evolution of bioactive silk with improved functional properties.
Systemic Embolism and Clinically Significant Bleeding Events in Older Adults with Nonvalvular Atrial Fibrillation After Treatment with Direct Oral Anticoagulants and Warfarin: A Retrospective Cohort Study in Japan
Authors: Yaguchi Daichi , Sera Shoji , Okada Akira , Nishimura Yuki , Tamaru Satoshi , Nagai Naomi
Published: November 2024

Background/Objectives: Anticoagulant therapy, particularly the use of direct oral anticoagulant agents (DOACs), is recommended for patients with nonvalvular atrial fibrillation (NVAF). This multicenter observational retrospective cohort study aimed to assess the efficacy and safety of DOACs compared to warfarin in Japanese patients aged 75 years and older with NVAF. Methods: Data from the Mie-Life Innovation Promotion Center Database were used to collect medical information on the patients. The cumulative incidences of clinically significant bleeding events and systemic embolic events (SEEs) were analyzed. Results: This study included 1787 older adult patients, of whom 1321 received DOACs (edoxaban: 428; apixaban: 586; dabigatran: 105; rivaroxaban: 202) and 466 receiving warfarin. There were no statistically significant differences in the cumulative incidence of bleeding events between the DOAC- and warfarin-treated groups. However, a statistically significant difference was observed for SEEs, with dabigatran showing a significantly lower incidence compared to warfarin. Conclusions: The incidence rates of bleeding events for individual DOACs were comparable to those for warfarin. Additionally, a history of vascular disorders was identified as a risk factor for bleeding events in the DOAC-treated group (hazard ratio (HR): 1.83, 95% confidence interval (CI): 1.16–2.88, p < 0.01) and warfarin-treated group (HR: 1.80, 95% CI: 1.15–2.84, p < 0.01). Based on real-world data, the overall efficacy and safety of DOACs were generally comparable to warfarin.
Cannabidiol Supplements in Romania: Bridging the Gap Between Marketed Claims and Clinical Reality
Authors: Andrei Corina , Nitulescu George Mihai , Nitulescu Georgiana , Zanfirescu Anca
Published: November 2024

In recent years, the European market, including Romania, has witnessed a significant increase in the promotion of cannabidiol (CBD)-based products, often presented as effective treatments for various health conditions. This study investigates the inconsistencies between the health claims associated with these supplements and the evidence from clinical trials. To identify products available on the Romanian market, a systematic review of online pharmacies and websites that specialize in selling CBD-based products has been performed. Additionally, a systematic review of clinical trials has been conducted to assess the efficacy of CBD for the specified indications. Our analysis revealed that some claims, such as those related to post-traumatic stress disorder, lack substantial clinical evidence. Moreover, even when clinical support exists, the dosages recommended for the supplements are often significantly lower than those used in trials, raising concerns about their efficacy. These findings highlight the need for stricter regulatory oversight and more transparent communication to ensure that consumer expectations are aligned with scientific evidence, ultimately promoting informed decision-making and consumer safety.