bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Different Yeast Strains on Biogenic Amines, Volatile Compounds and Sensory Profile of Beer
Authors: Matukas Mazvydas , Starkute Vytaute , Zokaityte Egle , Zokaityte Gintare , Klupsaite Dovile , Mockus Ernestas , Rocha João Miguel , Ruibys Romas , Bartkiene Elena
Published: August 2022

Nowadays, there are many sorts of beer, however, some of them, despite the good sensory and other quality indicators, could contain high concentrations of undesirable compounds, such biogenic amines (BA). The yeast strain (YS), used for fermentation, can cause desirable as well as undesirable changes in beer. The aim of this study was to evaluate the contribution of different YS (A-Saccharomyces cerevisiae var. diastaticus, B-Saccharomyces cerevisiae var. bayanus, C-Brettanomyces claussenii) on the main quality parameters of beer. In addition, the BA concentration and the volatile compounds (VC, measured by gas chromatography–mass spectrometry) and their relation with beer overall acceptability (OA, evaluated by 20 trained judges) and emotions induced for consumers were analysed. The YS was a significant factor on alcohol formation in beer (p = 0.0001). The highest colour intensity was shown by C beer (10.2 EBC), and the latter beer showed the lowest OA. All of the beer samples induced the highest intensity of the emotion “neutral”, and the main VC of the beer were 3-methyl-1-butanol; L-α-terpineol; hexanoic acid 3-methylbutyl ester; and n-capric acid isobutyl ester. The highest total BAs content was found in beer fermented with C. Finally, all of the tested YS are suitable for beer production, however, taking into consideration the safety aspect of the beer, it should be mentioned that the highest concentration of BAs was found in beer fermented with C strain.
Comprehensive Quality Evaluation for Medicinal and Edible Ziziphi Spinosae Semen before and after Rancidity Based on Traditional Sensory, Physicochemical Characteristics, and Volatile Compounds
Authors: Liu Zhenying , Xu Liang , Song Pingping , Wu Cui , Xu Bo , Li Zhuojun , Chao Zhimao
Published: August 2022

To comprehensively evaluate the quality of medicinal and edible Ziziphi Spinosae Semen (ZSS, the dried ripe seeds of Ziziphus jujuba var. spinosa) before and after rancidity during storage, some indicators including traditional sensory properties, physicochemical characteristics, and volatile compounds were analyzed. As a result, compared with the normal samples, the rancid samples of ZSS produced a darker color, a bitter taste, and an irritating odor, increased moisture content, electrical conductivity, fatty oil content, and acid value, and decreased water- and alcohol-soluble extract contents and pH value. Among them, the acid value had significant difference (p < 0.01) from 3.90 of normal ZSS to 18.68 mg/g of rancid ZSS. A total of 39 volatile compounds were identified in samples, including 20 in normal ZSS and 38 compounds in rancid ZSS. Nineteen common compounds were identified in normal and rancid samples. Among them, the content of 10 compounds such as δ-limonene, (R,R)-2,3-butanediol, and (R,S)-2,3-butanediol was decreased but that of nine compounds such as acetic acid, n-octanoic acid, and n-nonanoic acid was increased in rancid ZSS. Nineteen unique compounds such as β-phellandrene, α-pinene, and 3-carene were detected and only one compound, δ-cadinene, was not detected in rancid ZSS. In addition, eight short-chain organic acids, acetic, propanoic, butanoic, pentanoic, hexanoic, heptanoic, octanoic, and nonanoic acids, were new products in rancid ZSS, and it was speculated that the production of a series of organic acids might be the material basis of irritating odor after normal ZSS became rancid. This is the first report that a series of short-chain organic acids have been found in a rancid substance. In conclusion, there was a significant difference between normal and rancid ZSS. These indicators could be used as an early warning for judging the rancidity phenomenon of medicinal and edible ZSS. In addition, this is the first comprehensive evaluation about the rancidity process of a medicinal and edible substance.
Early Carboniferous Ostracods (Crustacea) from Death Valley, California, USA
Authors: McMenamin Mark A. S.
Published: August 2022

Silicified ostracods from the Tin Mountain Limestone provide new information regarding the Carboniferous paleontology of the Death Valley Region, California, USA. Acid maceration of marine limestones yielded the following ostracods: Acratia spp., Bairdia quasilecta, Bairdia sp. cf. B. orientalis, Ceratobairdia sp., Kirkbya panamintensis sp. nov., Rectobairdia sp. cf. R. legumen, and Silenites sp. This is the first report of Ceratobairdia and Silenites from the Tin Mountain Limestone. These ostracods occupied a carbonate ramp environment that formed during a major Paleozoic transgression. The ostracods played an important paleoecological role, likely as benthic marine scavengers in a shallow marine biotope along the northern shores of Pangea.
Research Foci in the History of Science in Past Islamicate Societies
Authors: Brentjes Sonja
Published: August 2022

In recent years, numerous changes have emerged in the History of Science of what has traditionally been called the Islamic world. By now, it has become usual to speak of the Islamicate world, albeit more so in Islamic Studies and related historical disciplines. The notion Islamicate wishes to express that the societies rule by Muslim dynasties were multi-cultural, multi-ethnic, multi-confessional and plurilingual. Different Muslim denominations could form majority but also minority groups. The processes of change in the study of the sciences in those societies can be summarized as efforts to pluralize research approaches and to historicize objects, themes, people, institutions and practices. The pluralization of approaches includes the multiplication of (1) modern disciplinary homes for studies of scientific topics dealt with in Islamicate societies, (2) the languages acknowledged as languages of scientific texts such as New Persian, Ottoman Turkish or Urdu worthwhile to analyze, (3) the number of historical disciplines accepted under the umbrella of history of science, (4) the centuries or periods as well as the regions that have been incorporated into the investigation of past scientific knowledge and (5) the recognition that more than a single history can and should be told about the sciences in past Islamicate societies. The process of historicization means, first and foremost, to turn away from macro-units of research (Islam, medieval or Arabic science) to medium- or micro-level units. Historicization indicates, secondly, the turn toward contextualization beyond the analysis of individual texts or instruments. And thirdly, it signifies the integration of features or aspects of scholarly practices that are not limited to the content of a discipline or a text but include layouts, the organization of text production, types of visualizations of knowledge or rhetorical strategies and paratextual elements. My paper reports on trends that I consider relevant for understanding how the field changed over the last decades and how it ticks today. But it does not try to be comprehensive.
SARS-CoV-2 Infection in Pregnancy: Placental Histomorphological Patterns, Disease Severity and Perinatal Outcomes
Authors: Wong Yin Ping , Tan Geok Chin , Omar Siti Zarqah , Mustangin Muaatamarulain , Singh Yogesh , Salker Madhuri S. , Abd Aziz Nor Haslinda , Shafiee Mohamad Nasir
Published: August 2022

The association between maternal COVID-19 infection, placental histomorphology and perinatal outcomes is uncertain. The published studies on how placental structure is affected after SARS-CoV-2 virus in COVID-19-infected pregnant women are lacking. We investigated the effects of maternal SARS-CoV-2 infection on placental histomorphology and pregnancy outcomes. A retrospective cohort study on 47 pregnant women with confirmed SARS-CoV-2 infection, matched with non-infected controls, was conducted. Relevant clinicopathological data and primary birth outcomes were recorded. Histomorphology and SARS-CoV-2 immunohistochemistry analyses of placental tissues were performed. Only 1 of 47 cases showed SARS-CoV-2 immunoreactivity in the syncytiotrophoblasts. Histologically, decidual vasculopathy (n = 22/47, p = 0.004), maternal vascular thrombosis (n = 9/47, p = 0.015) and chronic histiocytic intervillositis (n = 10/47, p = 0.027) were significantly higher in the COVID-19-infected placentas when compared to the control group. Maternal vascular thrombosis was a significant feature in the active COVID-19 group. A significant lower gestational age (p < 0.001)) at delivery and a higher caesarean section rate (p = 0.007) were observed in the active SARS-CoV-2-infected cases, resulting in a significant lower fetal-placental weight ratio (p = 0.022) and poorer Apgar score (p < 0.001). Notably, active (p = 0.027), symptomatic (p = 0.039), severe-critical (p = 0.002) maternal COVID-19 infection and placental inflammation (p = 0.011) were associated with an increased risk of preterm delivery. Altered placental villous maturation and severe-critical maternal COVID-19 infection were associated with an elevated risk of poor Apgar scores at birth (p = 0.018) and maternal mortality (p = 0.023), respectively.
The Effects of the Type of Information Played in Environmentally Themed Short Videos on Social Media on People’s Willingness to Protect the Environment
Authors: Zheng Shiyong , Cui Jiarong , Sun Chaojing , Li Jiaying , Li Biqing , Guan Weili
Published: August 2022

This study used a 2 × 2 experimental design to explore the effects of message type (non-narrative vs. narrative information) and social media metrics (high vs. low numbers of plays) of low-carbon-themed social media short videos on people’s willingness to protect the environment. Subjects completed questionnaires after viewing short videos that contained different message types and social media metrics, and a final sample of 295 cases was included in the data analysis. The study found that, while the type of information (i.e., non-narrative or narrative) of the low-carbon-themed social media short videos had no direct effect on people’s willingness to protect the environment, its indirect effects were significant. These indirect effects were achieved through immersion experience and social influence. Subjects who watched narrative videos had a higher level of immersion experience, which in turn was significantly and positively correlated with environmental intention; meanwhile, those who watched non-narrative videos experienced a higher level of social influence, which in turn was significantly and positively correlated with environmental intention. In addition, subjects who viewed high-volume videos experienced a more positive effect on their willingness to protect the environment. This study explored how message design could promote subjects’ perceptions and positive attitudes towards environmental protection, with important managerial implications.
Smart Hand Sanitisers in the Workplace: A Survey of Attitudes towards an Internet of Things Technology
Authors: Madden Andrew D. , Rutter Sophie , Stones Catherine , Ai Wenbo
Published: August 2022

An online survey was circulated to employees from a wide range of organisations to gauge attitudes towards the idea of using smart hand sanitisers in the workplace. The sanitisers are capable of real-time monitoring and providing feedback that varies according to the hand hygiene behaviour of users. In certain circumstances, the sanitisers can monitor individuals, making it possible to identify workers whose hand hygiene falls below a certain standard. The survey was circulated between July and August 2021 during the COVID-19 pandemic. Data gathered from 314 respondents indicated support for some features of the technology, but also indicated concern about invasions of privacy and the possibility of coercion. Attitudes towards the possible implementation of the technology varied significantly according to certain characteristics of the sample, but particularly with age. Respondents above the median age were more likely to support the use of data in ways that could facilitate the promotion and enforcement of hand hygiene practices.
Information Seeking and Processing during the Outbreak of COVID-19 in Taiwan: Examining the Effects of Emotions and Informational Subjective Norms
Authors: Li Shu-Chu Sarrina , Lo Shih-Yu , Wu Tai-Yee , Chen Te-Lin
Published: August 2022

Adopting the model of risk information seeking and processing (RISP) as a theoretical framework, the objective of this study was to investigate the factors that prompted individuals’ information-seeking and -processing behaviors during the COVID-19 pandemic in Taiwan. There were two unique aspects in this study: one was to adopt specific emotions to investigate the impact of negative emotions, and the other was to examine the effect of informational subjective norms (ISNs) on information-seeking and -processing behavior. An online survey was conducted by a professional polling company, and a stratified random sampling method was employed, using gender, age, education, personal income, and residential areas as strata to select participants. This study obtained 1100 valid questionnaires. The results showed that (1) risk perception did not exert any significant impacts on respondents’ perceived information insufficiency; (2) risk perception exerted a powerful impact on respondents’ ISNs, which, in turn, positively affected their information insufficiency; (3) the respondents who experienced fear were found to have a high probability of using a systematic-processing mode, while the respondents who experienced anger were more likely to adopt a heuristic-processing mode to process information; and (4) the use of a systematic-processing mode was positively associated, while the use of a heuristic-processing mode was negatively associated, with information-seeking behavior.
The Prediction of Public Risk Perception by Internal Characteristics and External Environment: Machine Learning on Big Data
Authors: Xie Qihui , Xue Yanan
Published: August 2022

Presently, the public’s perception of risk in terms of topical social issues is mainly measured quantitively using a psychological scale, but this approach is not accurate enough for everyday data. In this paper, we explored the ways in which public risk perception can be more accurately predicted in the era of big data. We obtained internal characteristics and external environment predictor variables through a literature review, and then built our prediction model using the machine learning of a BP neural network via three steps: the calculation of the node number of the implication level, a performance test of the BP neural network, and the computation of the weight of every input node. Taking the public risk perception of the Sino–US trade friction and the COVID-19 pandemic in China as research cases, we found that, according to our tests, the node number of the implication level was 15 in terms of the Sino–US trade friction and 14 in terms of the COVID-19 pandemic. Following this, machine learning was conducted, through which we found that the R2 of the BP neural network prediction model was 0.88651 and 0.87125, respectively, for the two cases, which accurately predicted the public’s risk perception of the data on a certain day, and simultaneously obtained the weight of every predictor variable in each case. In this paper, we provide comments and suggestions for building a model to predict the public’s perception of topical issues.
What Does It Take to Get Somebody Back to Work after Severe Acquired Brain Injury? Service Actions within the Vocational Intervention Program (VIP 2.0)
Authors: McRae Philippa , Kobel Conrad , Lukersmith Sue , Simpson Grahame
Published: August 2022

Little is known about service actions delivered in the complex intervention of vocational rehabilitation (VR) for people with severe acquired brain injury (ABI). Scale-up of the Vocational Intervention Program (VIP) across the 12 Community teams of the NSW Brain Injury Rehabilitation Program provided an opportunity to analyse the intensity and profile of actions delivered in providing VR programs. Seventy-two participants with severe TBI were supported in returning to either pre-injury employment (FastTrack, FT, n = 27) or new employment (NewTrack, NT, n = 50), delivered by two types of VR providers (Disability Employment Service DES; private providers). VR providers documented their service actions in hours and minutes, using the Case Management Taxonomy, adapted to VR. The NT pathway required significantly higher levels of intervention in comparison to FT (25 h, five minutes vs. 35 h, 30 min, p = 0.048, W = 446). Case coordination was the most frequent service action overall (41.7% of total time for FT, 42.3% for NT). DES providers recorded significantly greater amounts of time undertaking engagement, assessment and planning, and emotional/motivational support actions compared to private providers. Overall duration of the programs were a median of 46 weeks (NT) and 36 weeks (FT), respectively. This study helps illuminate the profile of VR interventions for people with severe TBI.
The Efficacy of Digital Cognitive–Behavioral Interventions in Supporting the Psychological Adjustment and Sleep Quality of Pregnant Women with Sub-Clinical Symptoms: A Systematic Review and Meta-Analysis
Authors: Mancinelli Elisa , Bassi Giulia , Gabrielli Silvia , Salcuni Silvia
Published: August 2022

The present meta-analysis investigated the overall and differential efficacy of digital cognitive–behavioral therapies (CBTs) vs. third-generation CBTs deployed to pregnant women in reducing sub-clinical depression, anxiety, and stress symptoms while fostering sleep quality and quality of life. A PRISMA-guided systematic search was used, including randomized controlled trials (RCTs) evaluating the above-mentioned interventions. Data were pooled using either the mean difference (MD) or standardized MD (SMD). Sub-group analyses were carried out when appropriate. The primary outcomes were depression, anxiety, and stress symptoms, as well as sleep quality and quality of life. The interventions’ acceptability was evaluated through the odds ratio (OR) of drop-out rates. Seven RCTs were included, comprising 1873 pregnant women. The results showed the interventions’ efficacy in terms of reducing depression symptoms (SMD = −0.36, CI = 0.61, −0.11, k = 9) at the endpoint, although it was not maintained at follow-up during the postpartum period. The interventions’ efficacy in terms of reducing anxiety symptoms (SMD = 1.96, CI = −2.72, −1.21, k = 3) at the endpoint was also significant, while having no effect on sleep quality. The interventions were well accepted (OR = 1.68; 95% CI = 0.84, 3.35; k = 7). Although no sound conclusions can be drawn concerning the joint or differential efficacy of the considered interventions, this study was useful in highlighting the need to develop evidence-based digital prevention programs for pregnant women with sub-clinical symptoms.
Spanish Paediatricians’ Knowledge, Attitudes and Practices Regarding Oral Health of Children under 6 Years of Age: A Cross-Sectional Study
Authors: Martínez-Beneyto Yolanda , Navarrete-García Celia , Serna-Muñoz Clara , Ausina-Márquez Veronica , Poza-Pascual Andrea , Expósito-Delgado Antonio Javier , Vicente Ascensión , Ortiz-Ruiz Antonio J.
Published: August 2022

Background: Early Childhood Caries (ECC) is a prevalent chronic pathology, and it has a negative impact on the oral and general health of the child patient. Aim: To evaluate the knowledge, attitudes and practices of Spanish paediatricians regarding early childhood caries according to the professional’s years of experience. Material and Methods: A cross-sectional questionnaire was conducted by Spanish paediatricians via WhatsApp and e-mails from January to April 2021. Data were analysed using Chi-squared test, Fisher’s exact test and Cramer’s V test. Results: There were a total of 359 participants. Most respondents were women (81.3%) with up to 10 years of professional experience (31.2%) in primary health care and public health. In most cases, participants had an excellent knowledge of primary dentition (90.8%), but they ignored (56%) when the first visit to the dentist should occur. Regarding the aetiological factors of caries, oral hygiene and prevention, a lower rate of knowledge was observed. The majority of participants (80.8%) were not able to identify white spot lesions and enamel defects (76%). They considered that their knowledge in oral health was deficient, highlighting the need to increase their training. Less experienced paediatricians were found to have higher success rates. Conclusions: The level of knowledge and attitudes regarding early childhood caries of the evaluated paediatricians should be improved. Paediatricians had difficulties in identifying early caries lesions and enamel defects. Nevertheless, a higher level of knowledge and positive attitudes towards dental caries has been detected among paediatricians with fewer years of professional experience.
Comparison of Cholangiocarcinoma and Hepatocellular Carcinoma Incidence Trends from 1993 to 2012 in Lampang, Thailand
Authors: Cao Pianpian , Rozek Laura S. , Pongnikorn Donsuk , Sriplung Hutcha , Meza Rafael
Published: August 2022

Liver cancer is the most common cancer in Northern Thailand, mainly due to the dietary preference for raw fish, which can lead to infection by the parasite, O. viverrini, a causal agent of cholangiocarcinoma. We conducted a temporal trend analysis of cross-sectional incidence rates of liver cancer in Lampang, Northern Thailand. Liver cancer data from 1993–2012 were extracted from Lampang Cancer Registry. The multiple imputation by chained equations method was used to impute missing histology data. Imputed data were analyzed using Joinpoint and age-period-cohort (APC) models to characterize the incidence rates by gender, region, and histology, considering hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA). We observed a significant annual increase in CCA incidence and a considerable decrease in HCC incidence for both genders in Lampang. The APC analysis suggested that CCA incidence rates were higher in older ages, younger cohorts, and later years of diagnosis. In contrast, HCC incidence rates were higher in older generations and earlier years of diagnosis. Further studies of potential risk factors of CCA are needed to better understand and address the increasing burden of CCA in Lampang. Our findings may help to draw public attention to cholangiocarcinoma prevention and control in Northern Thailand.
Effectiveness of Whole-Body High-Intensity Interval Training on Health-Related Fitness: A Systematic Review and Meta-Analysis
Authors: Scoubeau Corentin , Bonnechère Bruno , Cnop Miriam , Faoro Vitalie , Klass Malgorzata
Published: August 2022

Due to its versatility, whole-body high-intensity interval training (WB-HIIT) can be proposed to the general population and patients to improve health-related fitness. However, its effectiveness compared to traditional aerobic continuous or interval trainings has yet to be determined. A search of four electronic databases was conducted. Studies reporting the effects of WB-HIIT on cardiorespiratory fitness (CRF), fat mass, fat-free mass, musculoskeletal fitness and metabolic risk factors were included. Standardized mean differences (SMD) between WB-HIIT and no exercise or traditional aerobic trainings were calculated. A meta-regression assessed the effect of total training time on the different outcomes. Twenty-two studies were included in the systematic review and nineteen in the meta-analysis. Compared to no exercise, WB-HIIT improves CRF (SMD: 0.75; 95%CI: 0.28, 1.23; p < 0.001), fat-free mass (SMD: 0.38; 95%CI: 0.11, 0.65; p < 0.001), fat mass (SMD: 0.40; 95%CI: 0.09, 0.72; p < 0.001) and musculoskeletal fitness (SMD: 0.84; 95%CI: 0.61, 1.08; p < 0.001). Compared to other aerobic trainings, WB-HIIT has a lower effect on CRF (SMD: −0.40; 95%CI: −0.70, −0.11; p = 0.007), a similar effect on fat-free mass (SMD: −0.04; 95%CI: −0.44, 0.35; p = 0.8) and fat mass (SMD: −0.07; 95%CI: −0.39, 0.25; p = 0.7), and a larger effect on musculoskeletal fitness (SMD: 0.42; 95%CI: 0.14, 0.71; p = 0.003). WB-HIIT overall effect and specific effect on CRF and fat mass were associated with total training time. The systematic review did not provide evidence of metabolic risk improvement. Despite a slightly lower effect on CRF, WB-HIIT is equally effective as traditional aerobic trainings to improve body composition and more effective to enhance musculoskeletal fitness, which is essential for execution of daily tasks.
How Do Male Football Players Meet Dietary Recommendations? A Systematic Literature Review
Authors: Danielik Karol , Książek Anna , Zagrodna Aleksandra , Słowińska-Lisowska Małgorzata
Published: August 2022

The aim of this review was to determine whether male football players meet dietary recommendations according to a UEFA expert group statement and to identify priority areas for dietetic intervention, including training periodization and field position. A database search of PubMed, Web of Science, EBSCO and Scopus was performed. To be included within the final review, articles were required to provide a dietary intake assessment of professional and semi-professional football players. A total of 17 studies met the full eligibility criteria. Several studies showed insufficient energy and carbohydrate intake compared to the recommendations. A majority of athletes consume adequate protein and fat intakes compared to the recommendations. In addition, several studies showed the insufficient intake of vitamins and minerals. This systematic review showed that football players do not meet the nutritional recommendations according to the UEFA expert group statement. Future research should be focused on how to apply nutritional recommendations specific for athletes in accordance with training periodization and positions on the field.
Palliative Care Knowledge and Attitudes towards End-of-Life Care among Undergraduate Nursing Students at Al-Quds University: Implications for Palestinian Education
Authors: Alwawi Abdallah Ahmad , Abu-Odah Hammoda , Bayuo Jonathan
Published: August 2022

(1) Background: Nursing students should be well-prepared and educated in order to provide high-quality palliative care (PC) to patients with chronic diseases, which will have an impact on the quality of care for dying patients in the future; this study aimed to investigate the level of knowledge about PC and attitudes towards the care of dying patients among Palestinian nursing undergraduate students; (2) Methods: The study was a descriptive quantitative cross-sectional study design of 410 nursing students who participated and completed the questionnaire. The PC Quiz for Nurses (PCQN) and the Short Version of the Frommelt Attitudes Towards Care of the Dying (FATCOD) Form B Scales were used to assess students’ PC knowledge and attitudes toward PC and caring for dying patients. Generalized linear regression analysis was applied to identify the influencing variables on knowledge and attitudes; (3) Results: The overall knowledge mean score was 7.42 ± 2.93, ranging from 0 to 20, which indicates that nursing students lacked knowledge about PC; however, they have a positive attitude (25.94 ± 4.72; ranging from 9 to 45) toward care for a dying person. Receiving lectures or training about PC, caring for relatives in their last days of life, gender, and year of study were independently associated with students’ knowledge and attitudes about PC and care of dying patients; and (4) Conclusion: This study generated evidence showing insufficient knowledge about PC among nursing students at the Al-Quds University, Palestine, albeit a favorable attitude towards EoLC was shown. Integrating PC education into the nursing curriculum at Palestinian Universities need to increase their awareness of PC.
Physical Exercise and Cognitive Function
Authors: Pastor Diego , Ballester-Ferrer Juan Arturo , Carbonell-Hernández Laura , Baladzhaeva Sabina , Cervello Eduardo
Published: August 2022

Cognitive skills are relevant predictors of academic achievement, employability, socioeconomic success, health, and longevity [...]
Raster Map Line Element Extraction Method Based on Improved U-Net Network
Authors: Ran Wenjing , Wang Jiasheng , Yang Kun , Bai Ling , Rao Xun , Zhao Zhe , Xu Chunxiao
Published: August 2022

To address the problem of low accuracy in line element recognition of raster maps due to text and background interference, we propose a raster map line element recognition method based on an improved U-Net network model, combining the semantic segmentation algorithm of deep learning, the attention gates (AG) module, and the atrous spatial pyramid pooling (ASPP) module. In the proposed network model, the encoder extracts image features, the decoder restores the extracted features, the features of different scales are extracted in the dilated convolution module between the encoder and the decoder, and the attention mechanism module increases the weight of line elements. The comparison experiment was carried out through the constructed line element recognition dataset. The experimental results show that the improved U-Net network accuracy rate is 93.08%, the recall rate is 92.29%, the DSC accuracy is 93.03%, and the F1-score is 92.68%. In the network robustness test, under different signal-to-noise ratios (SNRs), comparing the improved network structure with the original network structure, the DSC improved by 13.18–17.05%. These results show that the network model proposed in this paper can effectively extract raster map line elements.
Aquatic Freshwater Vertebrate Models of Epilepsy Pathology: Past Discoveries and Future Directions for Therapeutic Discovery
Authors: Williams Rachel E. , Mruk Karen
Published: August 2022

Epilepsy is an international public health concern that greatly affects patients’ health and lifestyle. About 30% of patients do not respond to available therapies, making new research models important for further drug discovery. Aquatic vertebrates present a promising avenue for improved seizure drug screening and discovery. Zebrafish (Danio rerio) and African clawed frogs (Xenopus laevis and tropicalis) are increasing in popularity for seizure research due to their cost-effective housing and rearing, similar genome to humans, ease of genetic manipulation, and simplicity of drug dosing. These organisms have demonstrated utility in a variety of seizure-induction models including chemical and genetic methods. Past studies with these methods have produced promising data and generated questions for further applications of these models to promote discovery of drug-resistant seizure pathology and lead to effective treatments for these patients.
The Biochemical Mechanism of Fork Regression in Prokaryotes and Eukaryotes—A Single Molecule Comparison
Authors: Bianco Piero R.
Published: August 2022

The rescue of stalled DNA replication forks is essential for cell viability. Impeded but still intact forks can be rescued by atypical DNA helicases in a reaction known as fork regression. This reaction has been studied at the single-molecule level using the Escherichia coli DNA helicase RecG and, separately, using the eukaryotic SMARCAL1 enzyme. Both nanomachines possess the necessary activities to regress forks: they simultaneously couple DNA unwinding to duplex rewinding and the displacement of bound proteins. Furthermore, they can regress a fork into a Holliday junction structure, the central intermediate of many fork regression models. However, there are key differences between these two enzymes. RecG is monomeric and unidirectional, catalyzing an efficient and processive fork regression reaction and, in the process, generating a significant amount of force that is used to displace the tightly-bound E. coli SSB protein. In contrast, the inefficient SMARCAL1 is not unidirectional, displays limited processivity, and likely uses fork rewinding to facilitate RPA displacement. Like many other eukaryotic enzymes, SMARCAL1 may require additional factors and/or post-translational modifications to enhance its catalytic activity, whereas RecG can drive fork regression on its own.
Synthetic Peucedanocoumarin IV Prevents α-Synuclein Neurotoxicity in an Animal Model of Parkinson’s Disease
Authors: Kim Heejeong , Maeng Han-Joo , Kim Ji Hun , Yoon Jin-Ha , Oh Yohan , Paek Seung-Mann , Lee Yunjong
Published: August 2022

Pathological protein inclusion formation and propagation are the main causes of neuronal dysfunction in diverse neurodegenerative diseases; therefore, current disease-modifying therapeutic strategies have targeted this disease protein aggregation process. Recently, we reported that peucedanocoumarin III (PCiii) is a promising therapeutic compound with the ability to disaggregate α-synuclein inclusion and protect dopaminergic neurons in Parkinson’s disease (PD). Here, we found that trans-4′-acetyl-3′-tigloylkhellactone (racemic peucedanocoumarin IV [PCiv]), a structural isomer of PCiii with a higher synthetic yield presented a strong anti-aggregate activity to a degree comparable to that of PCiii. PCiv retained effective inhibitory function against β-sheet aggregate-mimic β23 cytotoxicities and potently prevented α-synucleinopathy in α-synuclein preformed fibril (PFF)-treated mice cortical neurons. In detailed pharmacokinetic profiling of PCiv, oral administration of PCiv in rats exhibited an approximately 97-min half-life and 10% bioavailability. Moreover, tissue distribution analysis revealed favorable profiles of brain penetration with a 6.4 brain-to-plasma concentration ratio. The therapeutic efficacy of PCiv was further evaluated in a sporadic PD mouse model with a combinatorial co-injection of α-synuclein preformed fibril and recombinant adeno-associated virus expressing α-synuclein. Motor dysfunctions induced in this combinatorial α-synucleinopathy PD mouse model was almost completely rescued by PCiv diet administration, and this therapeutic effect is consistent with the marked prevention of dopaminergic neuron loss and suppression of α-synuclein aggregation. Taken together, our translational study suggests that PCiv is advantageous as a therapeutic agent for neurodegenerative diseases, especially with its good synthetic yield, high brain distribution, and anti-aggregate activity. PCiv may be useful in the management of α-synuclein inclusion formation and propagation at different stages of PD.
Candidate Genes in Testing Strategies for Linkage Analysis and Bioinformatic Sorting of Whole Genome Sequencing Data in Three Small Japanese Families with Idiopathic Superior Oblique Muscle Palsy
Authors: Matsuo Toshihiko , Chaomulige  , Miyaji Mary , Hosoya Osamu , Saito Akira , Nakazono Kazuyuki
Published: August 2022

Idiopathic superior oblique muscle palsy is a major type of paralytic, non-comitant strabismus and presents vertical and cyclo-torsional deviation of one eye against the other eye, with a large vertical fusion range and abnormal head posture such as head tilt. Genetic background is considered to play a role in its development, as patients with idiopathic superior oblique muscle palsy have varying degrees of muscle hypoplasia and, rarely, the complete absence of the muscle, that is, aplasia. In this study, whole genome sequencing was performed, and single nucleotide variations and short insertions/deletions (SNVs/InDels) were annotated in two patients each in three small families (six patients in total) with idiopathic superior oblique muscle palsy, in addition to three normal individuals in one family. At first, linkage analysis was carried out in the three families and SNVs/InDels in chromosomal loci with negative LOD scores were excluded. Next, SNVs/InDels shared by the six patients, but not by the three normal individuals, were chosen. SNVs/InDels were further narrowed down by choosing low-frequency (<1%) or non-registered SNVs/InDels in four databases for the Japanese population, and then by choosing SNVs/InDels with functional influence, leading to one candidate gene, SSTR5-AS1 in chromosome 16. The six patients were heterozygous for 13-nucleotide deletion in SSTR5-AS1, except for one homozygous patient, while the three normal individuals were wild type. Targeted polymerase chain reaction (PCR) and direct sequencing of PCR products confirmed the 13-nucleotide deletion in SSTR5-AS1. In the face of newly-registered SSTR5-AS1 13-nucleotide deletion at a higher frequency in a latest released database for the Japanese population, the skipping of low-frequency and non-registration sorting still resulted in only 13 candidate genes including SSTR5-AS1 as common variants. The skipping of linkage analysis also led to the same set of 13 candidate genes. Different testing strategies that consisted of linkage analysis and simple unintentional bioinformatics could reach candidate genes in three small families with idiopathic superior oblique muscle palsy.
Synthesis and Immunological Evaluation of Mannosylated Desmuramyl Dipeptides Modified by Lipophilic Triazole Substituents
Authors: Petrović Peroković Vesna , Car Željka , Bušljeta Mia , Mihelec Danijela , Paurević Marija , Ivanković Siniša , Stojković Ranko , Ribić Rosana
Published: August 2022

Muramyl dipeptide (N-acetylmuramyl-L-alanyl-D-isoglutamine, MDP) is the smallest peptidoglycan fragment able to trigger an immune response by activating the NOD2 receptor. Structural modification of MDP can lead to analogues with improved immunostimulating properties. The aim of this work was to prepare mannosylated desmuramyl peptides (ManDMP) containing lipophilic triazole substituents to study their immunomodulating activities in vivo. The adjuvant activity of the prepared compounds was evaluated in the mouse model using ovalbumin as an antigen and compared to the MDP and referent adjuvant ManDMPTAd. The obtained results confirm that the α-position of D-isoGln is the best position for the attachment of lipophilic substituents, especially adamantylethyl triazole. Compound 6c exhibited the strongest adjuvant activity, comparable to the MDP and better than referent ManDMPTAd.
Could SGLT2 Inhibitors Improve Exercise Intolerance in Chronic Heart Failure?
Authors: Voorrips Suzanne N. , Saucedo-Orozco Huitzilihuitl , Sánchez-Aguilera Pablo I. , De Boer Rudolf A. , Van der Meer Peter , Westenbrink B. Daan
Published: August 2022

Despite the constant improvement of therapeutical options, heart failure (HF) remains associated with high mortality and morbidity. While new developments in guideline-recommended therapies can prolong survival and postpone HF hospitalizations, impaired exercise capacity remains one of the most debilitating symptoms of HF. Exercise intolerance in HF is multifactorial in origin, as the underlying cardiovascular pathology and reactive changes in skeletal muscle composition and metabolism both contribute. Recently, sodium-related glucose transporter 2 (SGLT2) inhibitors were found to improve cardiovascular outcomes significantly. Whilst much effort has been devoted to untangling the mechanisms responsible for these cardiovascular benefits of SGLT2 inhibitors, little is known about the effect of SGLT2 inhibitors on exercise performance in HF. This review provides an overview of the pathophysiological mechanisms that are responsible for exercise intolerance in HF, elaborates on the potential SGLT2-inhibitor-mediated effects on these phenomena, and provides an up-to-date overview of existing studies on the effect of SGLT2 inhibitors on clinical outcome parameters that are relevant to the assessment of exercise capacity. Finally, current gaps in the evidence and potential future perspectives on the effects of SGLT2 inhibitors on exercise intolerance in chronic HF are discussed.
Distinct Roles of Estrogen Receptors in the Regulation of Vitellogenin Expression in Orange-Spotted Grouper (Epinephelus coioides)
Authors: Ye Zhifeng , Zhao Tingting , Wei Qianhao , Lin Haoran , Zhang Yong , Li Shuisheng
Published: August 2022

During their breeding season, estrogen induces vitellogenin (VTG) production in the liver of teleost fish through estrogen receptors (ERs) that support oocyte vitellogenesis. There are at least three ER subtypes in teleost fish, but their roles in mediating E2-induced VTG expression have yet to be ascertained. In this study, we investigated the expression of vtgs and ers in the liver of orange-spotted grouper (Epinephelus coioides). Their expression levels were significantly increased in the breeding season and were upregulated by an estradiol (E2) injection in female fish, except for the expression of erβ1. The upregulation of vtgs, erα and erβ2 by E2 was also observed in primary hepatocytes, but these stimulatory effects could be abolished by ER antagonist ICI182780 treatment. Subsequent studies showed that ERβ antagonist Cyclofenil downregulated the E2-induced expression of vtg, erα, and erβ2, while the ERβ agonist DPN simulated their expression. Knockdown of erβ2 by siRNA further confirmed that ERβ2 mediated the E2-induced expression of vtgs and erα. To reveal the mechanism of ERβ2 in the regulation of erα expression, the erα promoter was cloned, and its activity was examined in cells. E2 treatment simulated the activity of the erα promoter in the presence of ERβ2. Deletions and site-directed mutations showed that the E2 up-regulated transcriptional activity of erα occurs through a classical half-estrogen response element- (ERE) dependent pathway. This study reveals the roles of ER subtypes in VTG expression in orange-spotted grouper and provides a possible explanation for the rapid and efficient VTG production in this species during the breeding season.
Identification of Diverse Stress-Responsive Xylem Sap Peptides in Soybean
Authors: Sin Wai-Ching , Lam Hon-Ming , Ngai Sai-Ming
Published: August 2022

Increasing evidence has revealed that plant secretory peptides are involved in the long-distance signaling pathways that help to regulate plant development and signal stress responses. In this study, we purified small peptides from soybean (Glycine max) xylem sap via o-chlorophenol extraction and conducted an in-depth peptidomic analysis using a mass spectrometry (MS) and bioinformatics approach. We successfully identified 14 post-translationally modified peptide groups belonging to the peptide families CEP (C-terminally encoded peptides), CLE (CLAVATA3/embryo surrounding region-related), PSY (plant peptides containing tyrosine sulfation), and XAP (xylem sap-associated peptides). Quantitative PCR (qPCR) analysis showed unique tissue expression patterns among the peptide-encoding genes. Further qPCR analysis of some of the peptide-encoding genes showed differential stress-response profiles toward various abiotic stress factors. Targeted MS-based quantification of the nitrogen deficiency-responsive peptides, GmXAP6a and GmCEP-XSP1, demonstrated upregulation of peptide translocation in xylem sap under nitrogen-deficiency stress. Quantitative proteomic analysis of GmCEP-XSP1 overexpression in hairy soybean roots revealed that GmCEP-XSP1 significantly impacts stress response-related proteins. This study provides new insights that root-to-shoot peptide signaling plays important roles in regulating plant stress-response mechanisms.
GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean
Authors: Su Bohong , Wu Haitao , Guo Yong , Gao Huawei , Wei Zhongyan , Zhao Yuyang , Qiu Lijuan
Published: August 2022

Soybean plant height and branching affect plant architecture and yield potential in soybean. In this study, the mutant dmbn was obtained by treating the cultivar Zhongpin 661 with ethylmethane sulfonate. The dmbn mutant plants were shorter and more branched than the wild type. The genetic analysis showed that the mutant trait was controlled by a semi-dominant gene. The candidate gene was fine-mapped to a 91 kb interval on Chromosome 9 by combining BSA-seq and linkage analysis. Sequence analysis revealed that Glyma.09g193000 encoding an Aux/IAA protein (GmIAA27) was mutated from C to T in the second exon of the coding region, resulting to amino acid substitution of proline to leucine. Overexpression of the mutant type of this gene in Arabidopsis thaliana inhibited apical dominance and promoted lateral branch development. Expression analysis of GmIAA27 and auxin response genes revealed that some GH3 genes were induced. GmIAA27 relies on auxin to interact with TIR1, whereas Gmiaa27 cannot interact with TIR1 owing to the mutation in the degron motif. Identification of this unique gene that controls soybean plant height and branch development provides a basis for investigating the mechanisms regulating soybean plant architecture development.
Effect of CB2 Stimulation on Gene Expression in Pediatric B-Acute Lymphoblastic Leukemia: New Possible Targets
Authors: Punzo Francesca , Argenziano Maura , Tortora Chiara , Di Paola Alessandra , Mutarelli Margherita , Pota Elvira , Di Martino Martina , Di Pinto Daniela , Marrapodi Maria Maddalena , Roberti Domenico , Rossi Francesca
Published: August 2022

Acute lymphoblastic leukemia type B (B-ALL) is the most common kind of pediatric leukemia, characterized by the clonal proliferation of type B lymphoid stem cells. Important progress in ALL treatments led to improvements in long-term survival; nevertheless, many adverse long-term consequences still concern the medical community. Molecular and cellular target therapies, together with immunotherapy, are promising strategies to overcome these concerns. Cannabinoids, enzymes involved in their metabolism, and cannabinoid receptors type 1 (CB1) and type 2 (CB2) constitute the endocannabinoid system, involved in inflammation, immune response, and cancer. CB2 receptor stimulation exerts anti-proliferative and anti-invasive effects in many tumors. In this study, we evaluated the effects of CB2 stimulation on B-ALL cell lines, SUP-B15, by RNA sequencing, Western blotting, and ELISA. We observe a lower expression of CB2 in SUP-B15 cells compared to lymphocytes from healthy subjects, hypothesizing its involvement in B-ALL pathogenesis. CB2 stimulation reduces the expression of CD9, SEC61G, TBX21, and TMSB4X genes involved in tumor growth and progression, and also negatively affects downstream intracellular pathways. Our findings suggest an antitumor role of CB2 stimulation in B-ALL, and highlight a functional correlation between CB2 receptors and specific anti-tumoral pathways, even though further investigations are needed.
Harnessing the Power of Purple Sweet Potato Color and Myo-Inositol to Treat Classic Galactosemia
Authors: Hagen-Lillevik Synneva , Johnson Joshua , Siddiqi Anwer , Persinger Jes , Hale Gillian , Lai Kent
Published: August 2022

Classic Galactosemia (CG) is a devastating inborn error of the metabolism caused by mutations in the GALT gene encoding the enzyme galactose-1 phosphate uridylyltransferase in galactose metabolism. Severe complications of CG include neurological impairments, growth restriction, cognitive delays, and, for most females, primary ovarian insufficiency. The absence of the GALT enzyme leads to an accumulation of aberrant galactose metabolites, which are assumed to be responsible for the sequelae. There is no treatment besides the restriction of dietary galactose, which does not halt the development of the complications; thus, additional treatments are sorely needed. Supplements have been used in other inborn errors of metabolism but are not part of the therapeutic regimen for CG. The goal of this study was to test two generally recognized as safe supplements (purple sweet potato color (PSPC) and myo-inositol (MI)) that may impact cellular pathways contributing to the complications in CG. Our group uses a GalT gene-trapped mouse model to study the pathophysiology in CG, which phenocopy many of the complications. Here we report the ability of PSPC to ameliorate dysregulation in the ovary, brain, and liver of our mutant mice as well as positive results of MI supplementation in the ovary and brain.
From Biomarkers to Precision Medicine in Neurodegenerative Diseases: Where Are We?
Authors: Tondo Giacomo , De Marchi Fabiola
Published: August 2022

The biomarkers era grew in the last two decades when several technical and methodological advances have improved the research in neurodegenerative diseases [...]