bluuebunny/crossref_metadata_2025 huggingface dataset:
Essential Oils and Their Constituents Targeting the GABAergic System and Sodium Channels as Treatment of Neurological Diseases
Authors: Wang Ze-Jun , Heinbockel Thomas
Published: May 2018

Essential oils and the constituents in them exhibit different pharmacological activities, such as antinociceptive, anxiolytic-like, and anticonvulsant effects. They are widely applied as a complementary therapy for people with anxiety, insomnia, convulsion, pain, and cognitive deficit symptoms through inhalation, oral administration, and aromatherapy. Recent studies show that essential oils are emerging as a promising source for modulation of the GABAergic system and sodium ion channels. This review summarizes the recent findings regarding the pharmacological properties of essential oils and compounds from the oils and the mechanisms underlying their effects. Specifically, the review focuses on the essential oils and their constituents targeting the GABAergic system and sodium channels, and their antinociceptive, anxiolytic, and anticonvulsant properties. Some constituents target transient receptor potential (TRP) channels to exert analgesic effects. Some components could interact with multiple therapeutic target proteins, for example, inhibit the function of sodium channels and, at the same time, activate GABAA receptors. The review concentrates on perspective compounds that could be better candidates for new drug development in the control of pain and anxiety syndromes.
Exploration of the Fluorescent Properties and the Modulated Activities against Sirtuin Fluorogenic Assays of Chromenone-Derived Natural Products
Authors: Wen Hui , Xue Nina , Wu Feng , He Yujun , Zhang Gang , Hu Zebin , Cui Huaqing
Published: May 2018

Chromenone-derived natural products include chromones (flavone, isoflavone) and coumarins. Chromenone compounds not only exhibit impressive biological activities, but also are an important resource of experimentally used fluorophores, such as, 7-amino-4-methylcoumarin (AMC). Various chromenone compounds have reported to have weak fluorescence, and this has the potential to interfere with the measurements during AMC fluorogenic assays and result in non-robust assay readouts. Several flavones and isoflavones were found as SIRT1 activators, while fluorogenic sirtuin assays utilized AMC labelled peptides as the substrates. In this study we investigated whether the fluorescent properties of chromenone-derived natural products interrupt the measurement of SIRT1/2 modulated activities. We found that the reported SIRT1 activators: flavones were detected with the SIRT1 activation activity, but isoflavones were not detected with SIRT1 activation activity, and instead that they were found to be fluorogenic compounds. Another chromenone compound, osthole, exhibited a moderate SIRT2 inhibitory activity with an IC50 of 10 μM. In conclusion, the fluorescent properties of these chromenone compounds do affect the measurement of the sirtuin activities of both inhibitors and activators. However, if the possible fluorescence properties are mitigated in the assay readout, these fluorogenic assays enable the screening of activity modulators.
Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis
Authors: Yabré Moussa , Ferey Ludivine , Somé Issa Touridomon , Gaudin Karen
Published: May 2018

The greening of analytical methods has gained increasing interest in the field of pharmaceutical analysis to reduce environmental impacts and improve the health safety of analysts. Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most widely used analytical technique involved in pharmaceutical drug development and manufacturing, such as the quality control of bulk drugs and pharmaceutical formulations, as well as the analysis of drugs in biological samples. However, RP-HPLC methods commonly use large amounts of organic solvents and generate high quantities of waste to be disposed, leading to some issues in terms of ecological impact and operator safety. In this context, greening HPLC methods is becoming highly desirable. One strategy to reduce the impact of hazardous solvents is to replace classically used organic solvents (i.e., acetonitrile and methanol) with greener ones. So far, ethanol has been the most often used alternative organic solvent. Others strategies have followed, such as the use of totally aqueous mobile phases, micellar liquid chromatography, and ionic liquids. These approaches have been well developed, as they do not require equipment investments and are rather economical. This review describes and critically discusses the recent advances in greening RP-HPLC methods dedicated to pharmaceutical analysis based on the use of alternative solvents.
Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells
Authors: Qian Yu , Song Jia-Le , Sun Peng , Yi Ruokun , Liu Honglin , Feng Xia , Park Kun-Young , Zhao Xin
Published: May 2018

This study investigated the enhanced antiproliferative effect of Lactobacillus casei strain Shirota (LcS) on geniposide actions in human oral squamous carcinoma HSC-3 cells. An MTT assay, flow cytometry, qPCR assay, western blot and HPLC were used for this study. The concentration of 1.0 × 106 CFU/mL of LcS had no effect on the HOK normal oral epithelial cells and HSC-3 cancer cells. The 25 and 50 µg/mL geniposide concentrations also had no impact on HOK normal oral epithelial cells, but they had remarkable inhibitory effects on the growth of HSC-3 cancer cells, which are enhanced in the presence of LcS. By the flow cytometry assay, the LcS-geniposide-H (1.0 × 106 CFU/mL LcS and 50 µg/mL geniposide)-treated HSC-3 cancer cells had the largest number of cells undergoing apoptosis compared to cells treated with other combinationsand obviously more than cells treated with only geniposide-H (50 µg/mL geniposide). Geniposide-H could increase the mRNA and protein expressions of caspase-3, caspase-8, caspase-9, Bax, p53, p21, IκB-α, Fas, FasL, TIMP-1, and TIMP-2 as well as decrease those of Bcl-2, Bcl-xL, HIAP-1, HIAP-2, NF-κB, COX-2, iNOS, MMP-2, and MMP-9 compared to other groups of cells, and LcS further enhanced these changes, with results that are greater than for the cells treated with only a high concentration of geniposide. The results of this study show thatLcS enhanced the antiproliferative effect of geniposide in HSC-3 cancer cells.
Assessment of Lactose-Free Diet on the Phalangeal Bone Mineral Status in Italian Adolescents Affected by Adult-Type Hypolactasia
Authors: Baldan Alessandro , Tagliati Sylvie , Saccomandi Daniela , Brusaferro Andrea , Busoli Laura , Scala Andrea , Malaventura Cristina , Maggiore Giuseppe , Borgna-Pignatti Caterina
Published: May 2018

Adult-type hypolactasia (ATH) is a clinical syndrome of primary lactase deficiency. A lactose-free diet is advisable to avoid the symptoms linked to the condition, but this potentially creates problems for optimal bone mineralization due to reduced calcium intake. To evaluate the effect of the lactose-free diet on the bone mineral status (BMS), we compared the phalangeal BMS of adolescents with ATH to that of peers on a normal diet. Also, we analyzed the correlations between BMS and dietary behavior, physical exercise, and calcium and vitamin D intake. A total of 102 cases and 102 healthy controls filled out a diet record and underwent phalangeal Quantitative Ultrasound (QUS). No difference in BMS was observed. The time spent on lactose-free diet (4.8 ± 3.1 years) was inversely correlated to the BMS. More than 98% of cases consumed lactose-free milk, but calcium and vitamin D intake were significantly lower. Calcium intake was correlated to physical exercise but not to BMS. Our results suggest that a lactose-free diet does not affect the phalangeal BMS of adolescents with primary lactase deficiency when their diet includes lactose-free cow’s milk. However, there is still a significantly lower calcium intake than in the population reference. The inverse correlation observed between the BMS and the time spent on a lactose-free diet suggests that a long-term follow-up is advisable.
Effects of Whey Protein Supplementation Pre- or Post-Resistance Training on Muscle Mass, Muscular Strength, and Functional Capacity in Pre-Conditioned Older Women: A Randomized Clinical Trial
Authors: Nabuco Hellen C. G. , Tomeleri Crisieli M. , Sugihara Junior Paulo , Fernandes Rodrigo R. , Cavalcante Edilaine F. , Antunes Melissa , Ribeiro Alex S. , Teixeira Denilson C. , Silva Analiza M. , Sardinha Luís B. , Cyrino Edilson S.
Published: May 2018

Aging is associated with sarcopenia and dynapenia, with both processes contributing to functional dependence and mortality in older adults. Resistance training (RT) and increased protein intake are strategies that may contribute to health improvements in older adults. Therefore, the aim was to investigate the effects of whey protein (WP) supplementation consumed either immediately pre- or post-RT on skeletal muscle mass (SMM), muscular strength, and functional capacity in pre-conditioned older women. Seventy older women participated in this investigation and were randomly assigned to one of three groups: whey protein pre-RT and placebo post-RT (WP-PLA, n = 24), placebo pre-RT and whey protein post-RT (PLA-WP, n = 23), and placebo pre- and post-RT (PLA-PLA, n = 23). Each group ingested 35 g of WP or PLA. The RT program was carried out over 12 weeks (three times per week; 3 × 8–12 repetition maximum). Body composition, muscular strength, functional capacity, and dietary intake were assessed pre- and post-intervention. Two-way analysis of covariance (ANCOVA) for repeated measures, with baseline scores as covariates were used for data analysis. A time vs. group interaction (p < 0.05) was observed with WP-PLA and PLA-WP presenting greater increases compared with PLA-PLA for SMM (WP-PLA = 3.4%; PLA-WP = 4.2%; PLA-PLA = 2.0%), strength (WP-PLA = 8.1%; PLA-WP = 8.3%; PLA-PLA = 7.0%), and the 10-m walk test (WP-PLA = −10.8%; PLA-WP = −11.8%; PLA-PLA = −4.3%). Whey protein supplementation was effective in promoting increases in SMM, muscular strength, and functional capacity in pre-conditioned older women, regardless of supplementation timing. This trial was registered at ClinicalTrials.gov: NCT03247192.
The Effect of Aromatic Diimide Side Groups on the π-Conjugated Polymer Properties
Authors: Drewniak Anna , Tomczyk Mateusz D. , Hanusek Lukasz , Mielanczyk Anna , Walczak Krzysztof , Nitschke Pawel , Hajduk Barbara , Ledwon Przemyslaw
Published: May 2018

The presented study describes the method for the synthesis and characterization of a new class of conjugated copolymers containing a perylenediimide (PDI) and naphthalene diimide (NDI) side groups. The main conjugated backbone is a donor-acceptor polymer poly[3,6-carbazole-alt-5,5-(4′,7′-di-2-thienyl-2′,1′,3′-benzothiadiazole)] containing thiophene and carbazole as donor units and benzothiadiazole as an acceptor unit. The presented compounds were synthesized in a multistep synthesis. The polymerization was carried out by Suzuki or Stille coupling reaction. Redox properties of the studied polymers were tested in different conditions. Electrochemical investigation revealed independent reduction of the main polymer chain and diimide side groups. UV-Vis spectroscopy revealed the overlap of two absorption spectra. The difference between the electron affinity of the polymer main chain and that of the diimides estimated electrochemically is approximately 0.3 eV.
Fructose-Based Acrylic Copolymers by Emulsion Polymerization
Authors: Desport Jessica S. , Moreno Mónica , Barandiaran María J.
Published: May 2018

The exploration of a renewable resource for the preparation of waterborne copolymers was conducted. Low molar mass sugar resources were selected for their wide availability. A fructose-based monomer (MF) bearing a methacrylate radically polymerizable group was successfully synthesized. The latter was shown to be able to homopolymerize in emulsion. The high Tg of the resulting polymer (about 115 °C) makes it of particular interest for adhesive and coating applications where hard materials are necessary to ensure valuable properties. As a result, its incorporation in waterborne acrylic containing formulations as an equivalent to petrochemical-based methyl methacrylate was investigated. It was found that the bio-based monomer exhibited similar behavior to that of common methacrylates, as shown by polymerization kinetics and particle size evolution. Furthermore, the homogeneous incorporation of the sugar units into the acrylate chains was confirmed by a unique glass transition temperature in differential scanning calorimeter (DSC). The potential of MF for the production of waterborne copolymers was greatly valued by the successful increase of formulation solids content up to 45 wt %. Interestingly, polymer insolubility in tetrahydrofurane increased with time due to further reactions occurring in storage. Most likely, the partial deprotection of sugar units was the reason for the creation of hydrogen bonding and, thus, physically insoluble entangled chains. This behavior highlights opportunities to make use of hydroxyl groups either for further functionalization or, eventually, for achieving enhanced adhesion on casted substrates.
Optimisation of the Adhesion of Polypropylene-Based Materials during Extrusion-Based Additive Manufacturing
Authors: Spoerk Martin , Gonzalez-Gutierrez Joamin , Lichal Christof , Cajner Hrvoje , Berger Gerald Roman , Schuschnigg Stephan , Cardon Ludwig , Holzer Clemens
Published: May 2018

Polypropylene (PP) parts produced by means of extrusion-based additive manufacturing, also known as fused filament fabrication, are prone to detaching from the build platform due to their strong tendency to shrink and warp. Apart from incorporating high volume fractions of fillers, one approach to mitigate this issue is to improve the adhesion between the first deposited layer and the build platform. However, a major challenge for PP is the lack of adhesion on standard platform materials, as well as a high risk of welding on PP-based platform materials. This study reports the material selection of build platform alternatives based on contact angle measurements. The adhesion forces, investigated by shear-off measurements, between PP-based filaments and the most promising platform material, an ultra-high-molecular-weight polyethylene (UHMW-PE), were optimised by a thorough parametric study. Higher adhesion forces were measured by increasing the platform and extrusion temperatures, increasing the flow rate and decreasing the thickness of the first layer. Apart from changes in printer settings, an increased surface roughness of the UHMW-PE platform led to a sufficient, weld-free adhesion for large-area parts of PP-based filaments, due to improved wetting, mechanical interlockings, and an increased surface area between the two materials in contact.
Rainfall Variability, Wetland Persistence, and Water–Carbon Cycle Coupling in the Upper Zambezi River Basin in Southern Africa
Authors: Lowman Lauren E. L. , Wei Tiffany M. , Barros Ana P.
Published: May 2018

The Upper Zambezi River Basin (UZRB) delineates a complex region of topographic, soil and rainfall gradients between the Congo rainforest and the Kalahari Desert. Satellite imagery shows permanent wetlands in low-lying convergence zones where surface–groundwater interactions are vigorous. A dynamic wetland classification based on MODIS Nadir BRDF-Adjusted Reflectance is developed to capture the inter-annual and seasonal changes in areal extent due to groundwater redistribution and rainfall variability. Simulations of the coupled water–carbon cycles of seasonal wetlands show nearly double rates of carbon uptake as compared to dry areas, at increasingly lower water-use efficiencies as the dry season progresses. Thus, wetland extent and persistence into the dry season is key to the UZRB’s carbon sink and water budget. Whereas groundwater recharge governs the expansion of wetlands in the rainy season under large-scale forcing, wetland persistence in April–June (wet–dry transition months) is tied to daily morning fog and clouds, and by afternoon land–atmosphere interactions (isolated convection). Rainfall suppression in July–September results from colder temperatures, weaker regional circulations, and reduced instability in the lower troposphere, shutting off moisture recycling in the dry season despite high evapotranspiration rates. The co-organization of precipitation and wetlands reflects land–atmosphere interactions that determine wetland seasonal persistence, and the coupled water and carbon cycles.
On the Beat Detection Performance in Long-Term ECG Monitoring Scenarios
Authors: Melgarejo-Meseguer Francisco-Manuel , Everss-Villalba Estrella , Gimeno-Blanes Francisco-Javier , Blanco-Velasco Manuel , Molins-Bordallo Zaida , Flores-Yepes José-Antonio , Rojo-Álvarez José-Luis , García-Alberola Arcadi
Published: May 2018

Despite the wide literature on R-wave detection algorithms for ECG Holter recordings, the long-term monitoring applications are bringing new requirements, and it is not clear that the existing methods can be straightforwardly used in those scenarios. Our aim in this work was twofold: First, we scrutinized the scope and limitations of existing methods for Holter monitoring when moving to long-term monitoring; Second, we proposed and benchmarked a beat detection method with adequate accuracy and usefulness in long-term scenarios. A longitudinal study was made with the most widely used waveform analysis algorithms, which allowed us to tune the free parameters of the required blocks, and a transversal study analyzed how these parameters change when moving to different databases. With all the above, the extension to long-term monitoring in a database of 7-day Holter monitoring was proposed and analyzed, by using an optimized simultaneous-multilead processing. We considered both own and public databases. In this new scenario, the noise-avoid mechanisms are more important due to the amount of noise that exists in these recordings, moreover, the computational efficiency is a key parameter in order to export the algorithm to the clinical practice. The method based on a Polling function outperformed the others in terms of accuracy and computational efficiency, yielding 99.48% sensitivity, 99.54% specificity, 99.69% positive predictive value, 99.46% accuracy, and 0.85% error for MIT-BIH arrhythmia database. We conclude that the method can be used in long-term Holter monitoring systems.
Robust Pedestrian Dead Reckoning Based on MEMS-IMU for Smartphones
Authors: Kuang Jian , Niu Xiaoji , Chen Xingeng
Published: May 2018

This paper proposes a pedestrian dead reckoning (PDR) algorithm based on the strap-down inertial navigation system (SINS) using the gyros, accelerometers, and magnetometers on smartphones. In addition to using a gravity vector, magnetic field vector, and quasi-static attitude, this algorithm employs a gait model and motion constraint to provide pseudo-measurements (i.e., three-dimensional velocity and two-dimensional position increment) instead of using only pseudo-velocity measurement for a more robust PDR algorithm. Several walking tests show that the advanced algorithm can maintain good position estimation compare to the existing SINS-based PDR method in the four basic smartphone positions, i.e., handheld, calling near the ear, swaying in the hand, and in a pants pocket. In addition, we analyze the navigation performance difference between the advanced algorithm and the existing gait-model-based PDR algorithm from three aspects, i.e., heading estimation, position estimation, and step detection failure, in the four basic phone positions. Test results show that the proposed algorithm achieves better position estimation when a pedestrian holds a smartphone in a swaying hand and step detection is unsuccessful.
Double-Group Particle Swarm Optimization and Its Application in Remote Sensing Image Segmentation
Authors: Shen Liang , Huang Xiaotao , Fan Chongyi
Published: May 2018

Particle Swarm Optimization (PSO) is a well-known meta-heuristic. It has been widely used in both research and engineering fields. However, the original PSO generally suffers from premature convergence, especially in multimodal problems. In this paper, we propose a double-group PSO (DG-PSO) algorithm to improve the performance. DG-PSO uses a double-group based evolution framework. The individuals are divided into two groups: an advantaged group and a disadvantaged group. The advantaged group works according to the original PSO, while two new strategies are developed for the disadvantaged group. The proposed algorithm is firstly evaluated by comparing it with the other five popular PSO variants and two state-of-the-art meta-heuristics on various benchmark functions. The results demonstrate that DG-PSO shows a remarkable performance in terms of accuracy and stability. Then, we apply DG-PSO to multilevel thresholding for remote sensing image segmentation. The results show that the proposed algorithm outperforms five other popular algorithms in meta-heuristic-based multilevel thresholding, which verifies the effectiveness of the proposed algorithm.
Handheld Real-Time LED-Based Photoacoustic and Ultrasound Imaging System for Accurate Visualization of Clinical Metal Needles and Superficial Vasculature to Guide Minimally Invasive Procedures
Authors: Xia Wenfeng , Kuniyil Ajith Singh Mithun , Maneas Efthymios , Sato Naoto , Shigeta Yusuke , Agano Toshitaka , Ourselin Sebastian , J. West Simeon , E. Desjardins Adrien
Published: May 2018

Ultrasound imaging is widely used to guide minimally invasive procedures, but the visualization of the invasive medical device and the procedure’s target is often challenging. Photoacoustic imaging has shown great promise for guiding minimally invasive procedures, but clinical translation of this technology has often been limited by bulky and expensive excitation sources. In this work, we demonstrate the feasibility of guiding minimally invasive procedures using a dual-mode photoacoustic and ultrasound imaging system with excitation from compact arrays of light-emitting diodes (LEDs) at 850 nm. Three validation experiments were performed. First, clinical metal needles inserted into biological tissue were imaged. Second, the imaging depth of the system was characterized using a blood-vessel-mimicking phantom. Third, the superficial vasculature in human volunteers was imaged. It was found that photoacoustic imaging enabled needle visualization with signal-to-noise ratios that were 1.2 to 2.2 times higher than those obtained with ultrasound imaging, over insertion angles of 26 to 51 degrees. With the blood vessel mimicking phantom, the maximum imaging depth was 38 mm. The superficial vasculature of a human middle finger and a human wrist were clearly visualized in real-time. We conclude that the LED-based system is promising for guiding minimally invasive procedures with peripheral tissue targets.
Force Trends and Pulsatility for Catheter Contact Identification in Intracardiac Electrograms during Arrhythmia Ablation
Authors: Rivas-Lalaleo David , Muñoz-Romero Sergio , Huerta Mónica , Erazo-Rodas Mayra , Sánchez-Muñoz Juan José , Rojo-Álvarez José Luis , García-Alberola Arcadi
Published: May 2018

The intracardiac electrical activation maps are commonly used as a guide in the ablation of cardiac arrhythmias. The use of catheters with force sensors has been proposed in order to know if the electrode is in contact with the tissue during the registration of intracardiac electrograms (EGM). Although threshold criteria on force signals are often used to determine the catheter contact, this may be a limited criterion due to the complexity of the heart dynamics and cardiac vorticity. The present paper is devoted to determining the criteria and force signal profiles that guarantee the contact of the electrode with the tissue. In this study, we analyzed 1391 force signals and their associated EGM recorded during 2 and 8 s, respectively, in 17 patients (82 ± 60 points per patient). We aimed to establish a contact pattern by first visually examining and classifying the signals, according to their likely-contact joint profile and following the suggestions from experts in the doubtful cases. First, we used Principal Component Analysis to scrutinize the force signal dynamics by analyzing the main eigen-directions, first globally and then grouped according to the certainty of their tissue-catheter contact. Second, we used two different linear classifiers (Fisher discriminant and support vector machines) to identify the most relevant components of the previous signal models. We obtained three main types of eigenvectors, namely, pulsatile relevant, non-pulsatile relevant, and irrelevant components. The classifiers reached a moderate to sufficient discrimination capacity (areas under the curve between 0.84 and 0.95 depending on the contact certainty and on the classifier), which allowed us to analyze the relevant properties in the force signals. We conclude that the catheter-tissue contact profiles in force recordings are complex and do not depend only on the signal intensity being above a threshold at a single time instant, but also on time pulsatility and trends. These findings pave the way towards a subsystem which can be included in current intracardiac navigation systems assisted by force contact sensors, and it can provide the clinician with an estimate of the reliability on the tissue-catheter contact in the point-by-point EGM acquisition procedure.
An Optimal Enhanced Kalman Filter for a ZUPT-Aided Pedestrian Positioning Coupling Model
Authors: Fan Qigao , Zhang Hai , Sun Yan , Zhu Yixin , Zhuang Xiangpeng , Jia Jie , Zhang Pengsong
Published: May 2018

Aimed at overcoming the problems of cumulative errors and low positioning accuracy in single Inertial Navigation Systems (INS), an Optimal Enhanced Kalman Filter (OEKF) is proposed in this paper to achieve accurate positioning of pedestrians within an enclosed environment. Firstly, the errors of the inertial sensors are analyzed, modeled, and reconstructed. Secondly, the cumulative errors in attitude and velocity are corrected using the attitude fusion filtering algorithm and Zero Velocity Update algorithm (ZUPT), respectively. Then, the OEKF algorithm is described in detail. Finally, a pedestrian indoor positioning experimental platform is established to verify the performance of the proposed positioning system. Experimental results show that the accuracy of the pedestrian indoor positioning system can reach 0.243 m, giving it a high practical value.
Nonlinear Effect of Financial Efficiency and Financial Competition on Heterogeneous Firm R&D: A Study on the Combined Perspective of Financial Quantity Expansion and Quality Development
Authors: Gao Yang , Wang Yu , Chu Chien-Chi , Tsai Sang-Bing , Du Xiaomin , Chen Quan
Published: May 2018

Manufacturing firm data and district financial quantity and quality indicators for 2005–2007 combined with heterogeneous firm characteristics were used with a threshold panel to study the effect of financial inefficiency on firm R&D and the financial boundaries of efficiency improvement. The results show that: (1) extensive financial quantity expansion cannot support high innovation efficiency R&D (Research and Development) activities in private enterprises, low- and medium-technology enterprises, and underdeveloped area enterprises, as it causes financial inefficiency problems and a shortage of R&D inputs; and (2) financial efficiency and financial competition have nonlinear effects on firm R&D. Financial inefficiency and either low or excessive financial competition result in a lack of highly efficient firm R&D. Only improvements in financial efficiency and moderate competition can significantly promote firm R&D. The results of this study reveal an important way to improve the influence of financial inefficiency on firm R&D by moving away from simply expanding financial quantity to promoting quality instead.
The Carbon Impact of International Tourists to an Island Country
Authors: Tsai Kang-Ting , Lin Tzu-Ping , Lin Yu-Hao , Tung Chien-Hung , Chiu Yi-Ting
Published: May 2018

Taiwan, located in the East Asia, is an island country with limited natural resources. To increase economic growth and reduce CO2 emission levels, the Taiwanese government is promoting a sustainable low-carbon tourism industry. This study investigated the CO2 emission coefficient of tourist activities and identified the CO2 emissions (CE) patterns of international visitors to Taiwan. The total CO2 emission per visitor without considering international transportation was estimated using a questionnaire. The total CO2 emission comprises the CO2 emission of transportation, the CO2 emission of accommodation, and the CO2 emission of tourist activities. The results suggest that more convenient public transportation might help to reduce the total CO2 emission. Without considering CO2 emission from international air travel, in contrast to many non-island countries where CO2 emission of transportation is the main contributor to total CO2 emission, the CO2 emission of accommodation was the main contributor to total CO2 emission in Taiwan. To reduce the CO2 emission of accommodation, the Taiwanese government should improve the energy-use efficiency of devices in tourist hotels and promote bed-and-breakfast accommodations with low CO2 emission coefficients. Visitors enjoyed culinary journeys and shopping, both of which are activities that contribute highly to the CO2 emission of tourist activities because of their high CO2 emission coefficients.
Factors Affecting Green Residential Building Development: Social Network Analysis
Authors: Yang Xiaodong , Zhang Jiayu , Zhao Xianbo
Published: May 2018

Green residential buildings (GRBs) are one of the effective practices of energy saving and emission reduction in the construction industry. However, many real estate developers in China are less willing to develop GRBs, because of the factors affecting green residential building development (GRBD). In order to promote the sustainable development of GRBs in China, this paper, based on the perspective of real estate developers, identifies the influential and critical factors affecting GRBD, using the method of social network analysis (SNA). Firstly, 14 factors affecting GRBD are determined from 64 preliminary factors of three main elements, and the framework is established. Secondly, the relationships between the 14 factors are analyzed by SNA. Finally, four critical factors for GRBD, which are on the local economy development level, development strategy and innovation orientation, developer’s acknowledgement and positioning for GRBD, and experience and ability for GRBD, are identified by the social network centrality test. The findings illustrate the key issues that affect the development of GRBs, and provide references for policy making by the government and strategy formulation by real estate developers.
Co-Producing a Vision and Approach for the Transition towards a Circular Economy: Perspectives from Government Partners
Authors: Velenturf Anne , Purnell Phil , Tregent Mike , Ferguson John , Holmes Alan
Published: May 2018

The United Kingdom’s (UK) economy is overly reliant on unsustainable production and consumption practices that deplete finite resources at rates that will increase production costs, business risk, and economic instability; it also produces emissions and waste that cause climate change and environmental degradation, impacting on well-being in the UK and beyond. The Resource Recovery from Waste programme (RRfW) promotes a transition towards waste and resource management in a circular economy that restores the environment, creates societal benefits, and promotes clean growth by engaging relevant actors in academia, government, and industry to co-produce a shared vision and approach that will realise such a transition. Sharing the RRfW’s government engagement results, this article presents a positive outlook for changing the UK economy and society through waste and resource management practices that maximise the values of materials by circulating them in the economy for as long as possible. Key themes, regulatory instruments, a stable policy framework, and an approach for effective academic–government collaboration are proposed. Comparing the results to government plans in four UK nations shows great differences in progress towards realising a circular economy. The article concludes with recommendations to capitalise on opportunities for growth, innovation, and resilient infrastructure whilst contributing to quality jobs and welfare throughout the UK.
Collection and Remanufacturing of Waste Products under Patent Protection and Government Regulation
Authors: Zhang Dingyue , Zhang Xuemei , Shi Bin , Cao Jian , Zhou Gengui
Published: May 2018

There is increasing academic and pragmatic interest in leveraging patent rights to invigorate remanufacturing for waste products under governmental interventions via regulations and reward–penalty instruments. In practice, many original manufacturers that are possessed with intellectual property rights allow third-party remanufacturers to implement reproducing operations through authorization and charging licensing fees. The general purpose of this paper is to explore favorable strategies for a closed-loop supply chain (CLSC) system of waste product collection and remanufacturing, in the context of either manufacturer-remanufacturing or remanufacturer-remanufacturing. To achieve such an objective, game theory is adopted to establish models of three collection and remanufacturing modes among channel members involving a manufacturer, a seller, and a remanufacturer. In so doing, the results show that a government’s allocations of elementary remanufacturing ratio and the unit amount of reward–penalty count significantly in CLSC operations, especially for the manufacturer, who acts as the leader in the system and makes mode selections.
Climate Change, Agriculture and Migration: A Survey
Authors: Falco Chiara , Donzelli Franco , Olper Alessandro
Published: May 2018

This paper proposes a selective review of the classical economics-based literature on climate change and migration, focusing on the extent to which agriculture might be considered a key mediating channel linking climate change to migration. Overall, climate change is expected to have large and negative effects on the global economy. These effects are even more evident whenever the economic sector considered is the agricultural one, particularly in developing countries. Hence, migration can be viewed as a specific form of adaptation implemented by individuals and households, enabling them to cope, among other things, with weather-induced risk. We show that the importance of agriculture emerges from both plenty of micro-level country studies and relatively few macro-level analyses using cross-sectional data over longer time periods. Thus, policy actions targeted to sustainable agriculture and rural development can both help tackle the challenges posed by climate change and create opportunities in the face of growing migration issues. However, we also stress that much of the current evidence is based on statistical associations that have nothing to do with causal inferences. This calls for the use of a more structural approach and more sophisticated research designs, enabling the researchers to better discriminate among different mechanisms concurrently at work. In addition, further research should be addressed to the role played by food security, a complex dimension largely missing in the current debates on climate change and migration.
Clickbait Convolutional Neural Network
Authors: Zheng Hai-Tao , Chen Jin-Yuan , Yao Xin , Sangaiah Arun Kumar , Jiang Yong , Zhao Cong-Zhi
Published: May 2018

With the development of online advertisements, clickbait spread wider and wider. Clickbait dissatisfies users because the article content does not match their expectation. Thus, clickbait detection has attracted more and more attention recently. Traditional clickbait-detection methods rely on heavy feature engineering and fail to distinguish clickbait from normal headlines precisely because of the limited information in headlines. A convolutional neural network is useful for clickbait detection, since it utilizes pretrained Word2Vec to understand the headlines semantically, and employs different kernels to find various characteristics of the headlines. However, different types of articles tend to use different ways to draw users’ attention, and a pretrained Word2Vec model cannot distinguish these different ways. To address this issue, we propose a clickbait convolutional neural network (CBCNN) to consider not only the overall characteristics but also specific characteristics from different article types. Our experimental results show that our method outperforms traditional clickbait-detection algorithms and the TextCNN model in terms of precision, recall and accuracy.
Why is Skeletal Muscle Regeneration Impaired after Myonecrosis Induced by Viperid Snake Venoms?
Authors: Gutiérrez José María , Escalante Teresa , Hernández Rosario , Gastaldello Stefano , Saravia-Otten Patricia , Rucavado Alexandra
Published: May 2018

Skeletal muscle regeneration after myonecrosis involves the activation, proliferation and fusion of myogenic cells, and a coordinated inflammatory response encompassing phagocytosis of necrotic cell debris, and the concerted synthesis of cytokines and growth factors. Myonecrosis often occurs in snakebite envenomings. In the case of venoms that cause myotoxicity without affecting the vasculature, such as those of many elapid snakes, regeneration proceeds successfully. In contrast, in envenomings by most viperid snakes, which affect the vasculature and extracellular matrix in addition to muscle fibers, regeneration is largely impaired and, therefore, the muscle mass is reduced and replaced by fibro-adipose tissue. This review discusses possible causes for such poor regenerative outcome including: (a) damage to muscle microvasculature, which causes tissue hypoxia and affects the inflammatory response and the timely removal of necrotic tissue; (b) damage to intramuscular nerves, which results in atrophy of regenerating fibers; (c) degradation of muscle cell basement membrane, compromising the spatial niche for proliferating myoblasts; (d) widespread degradation of the extracellular matrix; and (e) persistence of venom components in the damaged tissue, which may affect myogenic cells at critical points in the regenerative process. Understanding the causes of poor muscle regeneration may pave the way for the development of novel therapeutic interventions aimed at fostering the regenerative process in envenomed patients.
An Immunodominant Region of the Envelope Glycoprotein of Small Ruminant Lentiviruses May Function as Decoy Antigen
Authors: Zahno Marie-Luise , Bertoni Giuseppe
Published: May 2018

(1) Background: Small ruminant lentiviruses (SRLV) persist in infected goats that mount a strong humoral immune response characterized by low neutralizing titers. In this study, we characterized the antibody response to SU5, a variable, immunodominant epitope of the envelope glycoprotein of SRLV. We tested the working hypothesis that the variability of SU5 reflects escape from neutralizing antibody. (2) Methods: Affinity purified anti-SU5 antibody were tested for their neutralizing activity to the homologous lentivirus. Virus culture supernatant—in native form or following sonication and filtration—was used to test the ability of free envelope glycoproteins to compete for binding in a SU5-peptide-ELISA. (3) Results: Anti-SU5 antibodies are not neutralizing, strongly suggesting that they do not bind intact viral particles. In contrast, shed envelope glycoproteins efficiently compete for binding in a SU5-ELISA, providing convincing evidence that the SU5 epitope is exposed only on shed envelope glycoproteins. (4) Conclusions: Our results show that the antibody engaging SU5 is not neutralizing and does not appear to bind to SU expressed at the surface of virus particles. We propose that SU5 is a potential decoy epitope exposed on shaded envelope glycoproteins, luring the humoral immune response in committing an original antigenic sin to a functionally irrelevant epitope.
Involvement of Pro-Inflammatory Macrophages in Liver Pathology of Pirital Virus-Infected Syrian Hamsters
Authors: Campbell Corey L. , Phillips Aaron T. , Rico Amber , McGuire Amanda , Aboellail Tawfik A. , Quackenbush Sandra , Olson Ken E. , Schountz Tony
Published: May 2018

New World arenaviruses cause fatal hemorrhagic disease in South America. Pirital virus (PIRV), a mammarenavirus hosted by Alston’s cotton rat (Sigmodon alstoni), causes a disease in Syrian golden hamsters (Mesocricetus auratus) (biosafety level-3, BSL-3) that has many pathologic similarities to the South American hemorrhagic fevers (BSL-4) and, thus, is considered among the best small-animal models for human arenavirus disease. Here, we extend in greater detail previously described clinical and pathological findings in Syrian hamsters and provide evidence for a pro-inflammatory macrophage response during PIRV infection. The liver was the principal target organ of the disease, and signs of Kupffer cell involvement were identified in mortally infected hamster histopathology data. Differential expression analysis of liver mRNA revealed signatures of the pro-inflammatory response, hematologic dysregulation, interferon pathway and other host response pathways, including 17 key transcripts that were also reported in two non-human primate (NHP) arenavirus liver-infection models, representing both Old and New World mammarenavirus infections. Although antigen presentation may differ among rodent and NHP species, key hemostatic and innate immune-response components showed expression parallels. Signatures of pro-inflammatory macrophage involvement in PIRV-infected livers included enrichment of Ifng, Nfkb2, Stat1, Irf1, Klf6, Il1b, Cxcl10, and Cxcl11 transcripts. Together, these data indicate that pro-inflammatory macrophage M1 responses likely contribute to the pathogenesis of acute PIRV infection.
Probing Molecular Insights into Zika Virus–Host Interactions
Authors: Lee Ina , Bos Sandra , Li Ge , Wang Shusheng , Gadea Gilles , Desprès Philippe , Zhao Richard
Published: May 2018

The recent Zika virus (ZIKV) outbreak in the Americas surprised all of us because of its rapid spread and association with neurologic disorders including fetal microcephaly, brain and ocular anomalies, and Guillain–Barré syndrome. In response to this global health crisis, unprecedented and world-wide efforts are taking place to study the ZIKV-related human diseases. Much has been learned about this virus in the areas of epidemiology, genetic diversity, protein structures, and clinical manifestations, such as consequences of ZIKV infection on fetal brain development. However, progress on understanding the molecular mechanism underlying ZIKV-associated neurologic disorders remains elusive. To date, we still lack a good understanding of; (1) what virologic factors are involved in the ZIKV-associated human diseases; (2) which ZIKV protein(s) contributes to the enhanced viral pathogenicity; and (3) how do the newly adapted and pandemic ZIKV strains alter their interactions with the host cells leading to neurologic defects? The goal of this review is to explore the molecular insights into the ZIKV–host interactions with an emphasis on host cell receptor usage for viral entry, cell innate immunity to ZIKV, and the ability of ZIKV to subvert antiviral responses and to cause cytopathic effects. We hope this literature review will inspire additional molecular studies focusing on ZIKV–host Interactions.
Attenuated Phenotype and Immunogenic Characteristics of a Mutated Herpes Simplex Virus 1 Strain in the Rhesus Macaque
Authors: Fan Shengtao , Xu Xingli , Liao Yun , Wang Yongrong , Wang Jianbin , Feng Min , Wang Lichun , Zhang Ying , He Zhanlong , Yang Fengmei , Fraser Nigel W. , Li Qihan
Published: May 2018

Herpes simplex virus type 1(HSV-1) presents a conundrum to public health worldwide because of its specific pathogenicity and clinical features. Some experimental vaccines, such as the recombinant viral glycoproteins, exhibit the viral immunogenicity of a host-specific immune response, but none of these has achieved a valid epidemiological protective efficacy in the human population. In the present study, we constructed an attenuated HSV-1 strain M3 through the partial deletion of UL7, UL41, and the latency-associated transcript (LAT) using the CRISPR/Cas9 system. The mutant strain exhibited lowered infectivity and virulence in macaques. Neutralization testing and ELISpot detection of the specific T-cell responses confirmed the specific immunity induced by M3 immunization and this immunity defended against the challenges of the wild-type strain and restricted the entry of the wild-type strain into the trigeminal ganglion. These results in rhesus macaques demonstrated the potential of the attenuated vaccine for the prevention of HSV-1 in humans.
Avoiding the Use of Exhausted Drinking Water Filters: A Filter-Clock Based on Rusting Iron
Authors: Ndé-Tchoupé Arnaud Igor , Lufingo Mesia , Hu Rui , Gwenzi Willis , Ntwampe Seteno Karabo Obed , Noubactep Chicgoua , Njau Karoli N.
Published: May 2018

Efficient but affordable water treatment technologies are currently sought to solve the prevalent shortage of safe drinking water. Adsorption-based technologies are in the front-line of these efforts. Upon proper design, universally applied materials (e.g., activated carbons, bone chars, metal oxides) are able to quantitatively remove inorganic and organic pollutants as well as pathogens from water. Each water filter has a defined removal capacity and must be replaced when this capacity is exhausted. Operational experience has shown that it may be difficult to convince some low-skilled users to buy new filters after a predicted service life. This communication describes the quest to develop a filter-clock to encourage all users to change their filters after the designed service life. A brief discussion on such a filter-clock based on rusting of metallic iron (Fe0) is presented. Integrating such filter-clocks in the design of water filters is regarded as essential for safeguarding public health.
Pour une approche ergonomique de la conception d’un dispositif de formation à distance utilisant les TIC
Authors: Tricot André , Plégat-Soutjis Fabienne
Published: May 2018

Cet article présente ce que pourrait être une approche ergonomique de la conception d’un dispositif de formation utilisant les TIC. Plus qu’une méthode, qui indique aux concepteurs comment il faut concevoir, l’approche ergonomique présentée défend l’idée que l’on peut tenter d’améliorer le travail des concepteurs et des utilisateurs de tels dispositifs de formation en leur indiquant non pas comment faire, ni même quoi faire, mais plutôt en les aidant à élaborer et à partager une représentation commune du but et du processus de conception. Pour améliorer le travail des utilisateurs, il faudrait comprendre les différents aspects de leurs activités d’apprentissage et d’utilisation du dispositif, pour favoriser la mise en oeuvre de ces activités. L’article présente une liste de questions adressées aux concepteurs, censées les aider à élaborer en commun une représentation, à anticiper les problèmes des utilisateurs et à chercher des solutions.
Utilisations du manuel scolaire en version électronique par des enseignants en collège et lycée
Authors: Voulgre Emmanuelle
Published: May 2018

The paper presents a study on teachers’uses in secondary school of electronic versions of textbooks. With a qualitative analysis, we identify technical and educational benefits received by teachers. The study also identifies the needs expressed in terms of additional functionality expected.