bluuebunny/crossref_metadata_2025 huggingface dataset:
Reshaping the Healthcare Sector with Economic Policy Measures Based on COVID-19 Epidemic Severity: A Global Study
Authors: Jagrič Timotej , Fister Dušan , Jagrič Vita
Published: February 2022

Governments around the world are looking for ways to manage economic consequences of COVID-19 and promote economic development. The aim of this study is to identify the areas where the application of economic policy measures would enhance the resilience of societies on epidemic risks. We use data on the COVID-19 pandemic outcome in a large number of countries. With the estimation of multiple econometric models, we identify areas being a reasonable choice for economic policy intervention. It was found that viable remediation actions worth taking can be identified either for long-, mid-, or short-term horizons, impacting the equality, healthcare sector, and national economy characteristics. We suggest encouraging research and development based on innovative technologies linked to industries in healthcare, pharmaceutical, and biotech, promoting transformation of healthcare systems based on new technologies, providing access to quality healthcare, promoting public healthcare providers, and investing in the development of regional healthcare infrastructure, as a tool of equal regional development based on economic assessment. Further, a central element of this study, i.e. the innovative identification matrix, could be populated as a unique policy framework, either for latest pandemic or any similar outbreaks in future.
A Call for More Rigor in Science and Health Communication
Authors: Rudd Rima E.
Published: February 2022

Successful dissemination of scientific knowledge relies on the ability of the writer, speaker, and designer to provide information and data that is both available and accessible to the audience for whom it is intended. Scientific rigor, uniformly applied to the development of medicines, products, and devices must be applied, as well, to communications—spoken, written, posted, or displayed. Rigorous development and design protocols call for formative research data gathering, careful pilot testing with members of the intended audience, needed revisions, and rigorous assessments. Guidelines and tools developed for health literacy applications can be adopted and adapted for environmental health research and educational efforts in the design of questionnaires, instructions, education and report back materials, as well as for public discourse.
Why Physical Activity Should Be Considered in Clinical Trials for COVID-19 Vaccines: A Focus on Risk Groups
Authors: Bortolini Miguel Junior Sordi , Petriz Bernardo , Mineo José Roberto , Resende Rafael de Oliveira
Published: February 2022

Since the World Health Organization declared the global COVID-19 state of emergency in early 2020, several vaccine candidates have emerged to control SARS-CoV-2, and some of them have been approved and implemented in vaccination campaigns worldwide. Although clinical trials for these vaccines have been carried out using highly controlled methods with accurate immunological tests, clinical questionnaires did not include questions concerning the physical activity profile among volunteers. It has been well established that physical activity plays a pivotal role in the immune response after vaccination, led by the activation of cytokines, antibodies, and cells. This concept should have been considered when evaluating the efficacy of COVID-19 vaccine candidates, particularly in elderly and obese people. Here, we discuss data from the literature providing strong evidence regarding the importance of analyzing physical activity parameters to improve the accuracy of clinical trials on assessing the efficacy of vaccine candidates.
A Framework for Scaling Urban Transformative Resilience through Utilizing Volunteered Geographic Information
Authors: Moghadas Mahsa , Rajabifard Abbas , Fekete Alexander , Kötter Theo
Published: February 2022

Resilience in the urban context can be described as a continuum of absorptive, adaptive, and transformative capacities. The need to move toward a sustainable future and bounce forward after any disruption has led recent urban resilience initiatives to engage with the concept of transformative resilience when and where conventional and top-down resilience initiatives are less likely to deliver effective strategies, plans, and implementable actions. Transformative resilience pathways emphasize the importance of reflexive governance, inclusive co-creation of knowledge, innovative and collaborative learning, and self-organizing processes. To support these transformative pathways, considering techno-social co-evolution and digital transformation, using new data sources such as Volunteered Geographic Information (VGI) and crowdsourcing are being promoted. However, a literature review on VGI and transformative resilience reveals that a comprehensive understanding of the complexities and capacities of utilizing VGI for transformative resilience is lacking. Therefore, based on a qualitative content analysis of available resources, this paper explores the key aspects of using VGI for transformative resilience and proposes a comprehensive framework structured around the identified legal, institutional, social, economic, and technical aspects to formalize the process of adopting VGI in transformative resilience initiatives.
Up and Down γ-Synuclein Transcription in Dopamine Neurons Translates into Changes in Dopamine Neurotransmission and Behavioral Performance in Mice
Authors: Pavia-Collado Rubén , Rodríguez-Aller Raquel , Alarcón-Arís Diana , Miquel-Rio Lluís , Ruiz-Bronchal Esther , Paz Verónica , Campa Leticia , Galofré Mireia , Sgambato Véronique , Bortolozzi Analia
Published: February 2022

The synuclein family consists of α-, β-, and γ-Synuclein (α-Syn, β-Syn, and γ-Syn) expressed in the neurons and concentrated in synaptic terminals. While α-Syn is at the center of interest due to its implication in the pathogenesis of Parkinson’s disease (PD) and other synucleinopathies, limited information exists on the other members. The current study aimed at investigating the biological role of γ-Syn controlling the midbrain dopamine (DA) function. We generated two different mouse models with: (i) γ-Syn overexpression induced by an adeno-associated viral vector and (ii) γ-Syn knockdown induced by a ligand-conjugated antisense oligonucleotide, in order to modify the endogenous γ-Syn transcription levels in midbrain DA neurons. The progressive overexpression of γ-Syn decreased DA neurotransmission in the nigrostriatal and mesocortical pathways. In parallel, mice evoked motor deficits in the rotarod and impaired cognitive performance as assessed by novel object recognition, passive avoidance, and Morris water maze tests. Conversely, acute γ-Syn knockdown selectively in DA neurons facilitated forebrain DA neurotransmission. Importantly, modifications in γ-Syn expression did not induce the loss of DA neurons or changes in α-Syn expression. Collectively, our data strongly suggest that DA release/re-uptake processes in the nigrostriatal and mesocortical pathways are partially dependent on substantia nigra pars compacta /ventral tegmental area (SNc/VTA) γ-Syn transcription levels, and are linked to modulation of DA transporter function, similar to α-Syn.
The Role of Oncostatin M and Its Receptor Complexes in Cardiomyocyte Protection, Regeneration, and Failure
Authors: Kubin Thomas , Gajawada Praveen , Bramlage Peter , Hein Stefan , Berge Benedikt , Cetinkaya Ayse , Burger Heiko , Schönburg Markus , Schaper Wolfgang , Choi Yeong-Hoon , Richter Manfred
Published: February 2022

Oncostatin M (OSM), a member of the interleukin-6 family, functions as a major mediator of cardiomyocyte remodeling under pathological conditions. Its involvement in a variety of human cardiac diseases such as aortic stenosis, myocardial infarction, myocarditis, cardiac sarcoidosis, and various cardiomyopathies make the OSM receptor (OSMR) signaling cascades a promising therapeutic target. However, the development of pharmacological treatment strategies is highly challenging for many reasons. In mouse models of heart disease, OSM elicits opposing effects via activation of the type II receptor complex (OSMR/gp130). Short-term activation of OSMR/gp130 protects the heart after acute injury, whereas chronic activation promotes the development of heart failure. Furthermore, OSM has the ability to integrate signals from unrelated receptors that enhance fetal remodeling (dedifferentiation) of adult cardiomyocytes. Because OSM strongly stimulates the production and secretion of extracellular proteins, it is likely to exert systemic effects, which in turn, could influence cardiac remodeling. Compared with the mouse, the complexity of OSM signaling is even greater in humans because this cytokine also activates the type I leukemia inhibitory factor receptor complex (LIFR/gp130). In this article, we provide an overview of OSM-induced cardiomyocyte remodeling and discuss the consequences of OSMR/gp130 and LIFR/gp130 activation under acute and chronic conditions.
FBN2 Silencing Recapitulates Hypoxic Conditions and Induces Elastic Fiber Impairment in Human Dermal Fibroblasts
Authors: Boizot Jérémy , Minville-Walz Mélaine , Reinhardt Dieter Peter , Bouschbacher Marielle , Sommer Pascal , Sigaudo-Roussel Dominique , Debret Romain
Published: February 2022

Most chronic wounds are characterized by varying degrees of hypoxia and low partial pressures of O2 that may favor the development of the wound and/or delay healing. However, most studies regarding extracellular matrix remodeling in wound healing are conducted under normoxic conditions. Here, we investigated the consequences of hypoxia on elastic network formation, both in a mouse model of pressure-induced hypoxic ulcer and in human primary fibroblasts cultured under hypoxic conditions. In vitro, hypoxia inhibited elastic fiber synthesis with a reduction in fibrillin-2 expression at the mRNA and protein levels. Lysyl oxidase maturation was reduced, concomitant with lower enzymatic activity. Fibrillin-2 and lysyl oxidase could interact directly, whereas the downregulation of fibrillin-2 was associated with deficient lysyl oxidase maturation. Elastic fibers were not synthesized in the hypoxic inflammatory tissues resulting from in vivo pressure-induced ulcer. Tropoelastin and fibrillin-2 were expressed sparsely in hypoxic tissues stained with carbonic anhydrase IX. Different hypoxic conditions in culture resulted in the arrest of elastic fiber synthesis. The present study demonstrated the involvement of FBN2 in regulating elastin deposition in adult skin models and described the specific impact of hypoxia on the elastin network without consequences on collagen and fibronectin networks.
3D Printable Composite Biomaterials Based on GelMA and Hydroxyapatite Powders Doped with Cerium Ions for Bone Tissue Regeneration
Authors: Leu Alexa Rebeca , Cucuruz Andreia , Ghițulică Cristina-Daniela , Voicu Georgeta , Stamat (Balahura) Liliana-Roxana , Dinescu Sorina , Vlasceanu George Mihail , Stavarache Cristina , Ianchis Raluca , Iovu Horia , Costache Marieta
Published: February 2022

The main objective was to produce 3D printable hydrogels based on GelMA and hydroxyapatite doped with cerium ions with potential application in bone regeneration. The first part of the study regards the substitution of Ca2+ ions from hydroxyapatite structure with cerium ions (Ca10-xCex(PO4)6(OH)2, xCe = 0.1, 0.3, 0.5). The second part followed the selection of the optimal concentration of HAp doped, which will ensure GelMA-based scaffolds with good biocompatibility, viability and cell proliferation. The third part aimed to select the optimal concentrations of GelMA for the 3D printing process (20%, 30% and 35%). In vitro biological assessment presented the highest level of cell viability and proliferation potency of GelMA-HC5 composites, along with a low cytotoxic potential, highlighting the beneficial effects of cerium on cell growth, also supported by Live/Dead results. According to the 3D printing experiments, the 30% GelMA enriched with HC5 was able to generate 3D scaffolds with high structural integrity and homogeneity, showing the highest suitability for the 3D printing process. The osteogenic differentiation experiments confirmed the ability of 30% GelMA-3% HC5 scaffold to support and efficiently maintain the osteogenesis process. Based on the results, 30% GelMA-3% HC5 3D printed scaffolds could be considered as biomaterials with suitable characteristics for application in bone tissue engineering.
Genome-Wide Association Study on Root System Architecture and Identification of Candidate Genes in Wheat (Triticum aestivum L.)
Authors: Ma Jianhui , Zhao Dongyang , Tang Xiaoxiao , Yuan Meng , Zhang Daijing , Xu Mengyuan , Duan Yingze , Ren Haiyue , Zeng Qingdong , Wu Jianhui , Han Dejun , Li Tian , Jiang Lina
Published: February 2022

The root tissues play important roles in water and nutrient acquisition, environmental adaptation, and plant development. In this study, a diversity panel of 388 wheat accessions was collected to investigate nine root system architecture (RSA) traits at the three-leaf stage under two growing environments: outdoor pot culture (OPC) and indoor pot culture (IPC). Phenotypic analysis revealed that root development was faster under OPC than that under IPC and a significant correlation was observed between the nine RSA traits. The 660K single-nucleotide polymorphism (SNP) chip was used for a genome-wide association study (GWAS). Significant SNPs with a threshold of −log10 (p-value) ≥ 4 were considered. Thus, 36 quantitative trait loci (QTLs), including 13 QTL clusters that were associated with more than one trait, were detected, and 31 QTLs were first identified. The QTL clusters on chromosomes 3D and 5B were associated with four and five RSA traits, respectively. Two candidate genes, TraesCS2A01G516200 and TraesCS7B01G036900, were found to be associated with more than one RSA trait using haplotype analysis, and preferentially expressed in the root tissues. These favourable alleles for RSA traits identified in this study may be useful to optimise the root system in wheat.
Therapeutic Interventions to Mitigate Mitochondrial Dysfunction and Oxidative Stress–Induced Damage in Patients with Bipolar Disorder
Authors: Madireddy Sahithi , Madireddy Samskruthi
Published: February 2022

Bipolar disorder (BD) is characterized by mood changes, including recurrent manic, hypomanic, and depressive episodes, which may involve mixed symptoms. Despite the progress in neurobiological research, the pathophysiology of BD has not been extensively described to date. Progress in the understanding of the neurobiology driving BD could help facilitate the discovery of therapeutic targets and biomarkers for its early detection. Oxidative stress (OS), which damages biomolecules and causes mitochondrial and dopamine system dysfunctions, is a persistent finding in patients with BD. Inflammation and immune dysfunction might also play a role in BD pathophysiology. Specific nutrient supplements (nutraceuticals) may target neurobiological pathways suggested to be perturbed in BD, such as inflammation, mitochondrial dysfunction, and OS. Consequently, nutraceuticals may be used in the adjunctive treatment of BD. This paper summarizes the possible roles of OS, mitochondrial dysfunction, and immune system dysregulation in the onset of BD. It then discusses OS-mitigating strategies that may serve as therapeutic interventions for BD. It also analyzes the relationship between diet and BD as well as the use of nutritional interventions in the treatment of BD. In addition, it addresses the use of lithium therapy; novel antipsychotic agents, including clozapine, olanzapine, risperidone, cariprazine, and quetiapine; and anti-inflammatory agents to treat BD. Furthermore, it reviews the efficacy of the most used therapies for BD, such as cognitive–behavioral therapy, bright light therapy, imagery-focused cognitive therapy, and electroconvulsive therapy. A better understanding of the roles of OS, mitochondrial dysfunction, and inflammation in the pathogenesis of bipolar disorder, along with a stronger elucidation of the therapeutic functions of antioxidants, antipsychotics, anti-inflammatory agents, lithium therapy, and light therapies, may lead to improved strategies for the treatment and prevention of bipolar disorder.
A Novel Prophylaxis Strategy Using Liposomal Vaccine Adjuvant CAF09b Protects against Influenza Virus Disease
Authors: Zimmermann Julie , Schmidt Signe , Trebbien Ramona , Cox Rebecca , Zhou Fan , Follmann Frank , Pedersen Gabriel , Christensen Dennis
Published: February 2022

The SARS-CoV-2 pandemic caused a massive health and societal crisis, although the fast development of effective vaccines reduced some of the impact. To prepare for future respiratory virus pandemics, a pan-viral prophylaxis could be used to control the initial virus outbreak in the period prior to vaccine approval. The liposomal vaccine adjuvant CAF®09b contains the TLR3 agonist polyinosinic:polycytidylic acid, which induces a type I interferon (IFN-I) response and an antiviral state in the affected tissues. When testing CAF09b liposomes as a potential pan-viral prophylaxis, we observed that intranasal administration of CAF09b liposomes to mice resulted in an influx of innate immune cells into the nose and lungs and upregulation of IFN-I-related gene expression. When CAF09b liposomes were administered prior to challenge with mouse-adapted influenza A/Puerto Rico/8/1934 virus, it protected from severe disease, although the virus was still detectable in the lungs. However, when CAF09b liposomes were administered after influenza challenge, the mice had a similar disease course to controls. In conclusion, CAF09b may be a suitable candidate as a pan-viral prophylactic treatment for epidemic viruses, but must be administered prior to virus exposure to be effective.
Demystifying DPP III Catalyzed Peptide Hydrolysis—Computational Study of the Complete Catalytic Cycle of Human DPP III Catalyzed Tynorphin Hydrolysis
Authors: Tomić Antonija , Tomić Sanja
Published: February 2022

Dipeptidyl peptides III (DPP III) is a dual-domain zinc exopeptidase that hydrolyzes peptides of varying sequence and size. Despite attempts to elucidate and narrow down the broad substrate-specificity of DPP III, there is no explanation as to why some of them, such as tynorphin (VVYPW), the truncated form of the endogenous heptapeptide spinorphin, are the slow-reacting substrates of DPP III compared to others, such as Leu-enkephalin. Using quantum molecular mechanics calculations followed by various molecular dynamics techniques, we describe for the first time the entire catalytic cycle of human DPP III, providing theoretical insight into the inhibitory mechanism of tynorphin. The chemical step of peptide bond hydrolysis and the substrate binding to the active site of the enzyme and release of the product were described for DPP III in complex with tynorphin and Leu-enkephalin and their products. We found that tynorphin is cleaved by the same reaction mechanism determined for Leu-enkephalin. More importantly, we showed that the product stabilization and regeneration of the enzyme, but not the nucleophilic attack of the catalytic water molecule and inversion at the nitrogen atom of the cleavable peptide bond, correspond to the rate-determining steps of the overall catalytic cycle of the enzyme.
Central Nervous System Stimulants Limit Caffeine Transport at the Blood–Cerebrospinal Fluid Barrier
Authors: Ikeda-Murakami Kei , Tani Naoto , Ikeda Tomoya , Aoki Yayoi , Ishikawa Takaki
Published: February 2022

Caffeine, a common ingredient in energy drinks, crosses the blood–brain barrier easily, but the kinetics of caffeine across the blood–cerebrospinal fluid barrier (BCSFB) has not been investigated. Therefore, 127 autopsy cases (Group A, 30 patients, stimulant-detected group; and Group B, 97 patients, no stimulant detected group) were examined. In addition, a BCSFB model was constructed using human vascular endothelial cells and human choroid plexus epithelial cells separated by a filter, and the kinetics of caffeine in the BCSFB and the effects of 4-aminopyridine (4-AP), a neuroexcitatory agent, were studied. Caffeine concentrations in right heart blood (Rs) and cerebrospinal fluid (CSF) were compared in the autopsy cases: caffeine concentrations were higher in Rs than CSF in Group A compared to Group B. In the BCSFB model, caffeine and 4-AP were added to the upper layer, and the concentration in the lower layer of choroid plexus epithelial cells was measured. The CSF caffeine concentration was suppressed, depending on the 4-AP concentration. Histomorphological examination suggested that choroid plexus epithelial cells were involved in inhibiting the efflux of caffeine to the CSF. Thus, the simultaneous presence of stimulants and caffeine inhibits caffeine transfer across the BCSFB.
The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
Authors: Quintana-Sanchez Sara , Gómez-Casanova Natalia , Sánchez-Nieves Javier , Gómez Rafael , Rachuna Jarosław , Wąsik Sławomir , Semaniak Jacek , Maciejewska Barbara , Drulis-Kawa Zuzanna , Ciepluch Karol , Mata F. Javier de la , Arabski Michał
Published: February 2022

The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequate hydrophilic/hydrophobic structural balance to maximize the effect. Herein, we evaluated the antimicrobial activity of cationic carbosilane (CBS) dendrimers unmodified or modified with polyethylene glycol (PEG) units, against planktonic and biofilm-forming P. aeruginosa culture. Our study revealed that the presence of PEG destabilized the hydrophilic/hydrophobic balance but reduced the antibacterial activity measured by microbiological cultivation methods, laser interferometry and fluorescence microscopy. On the other hand, the activity can be improved by the combination of the CBS dendrimers with endolysin, a bacteriophage-encoded peptidoglycan hydrolase. This enzyme applied in the absence of the cationic CBS dendrimers is ineffective against Gram-negative bacteria because of the protective outer membrane shield. However, the endolysin—CBS dendrimer mixture enables the penetration through the membrane and then deterioration of the peptidoglycan layer, providing a synergic antimicrobial effect.
Juvenile Hormone Synthesis Pathway Gene SfIPPI Regulates Sogatella furcifera Reproduction
Authors: Gong Ming-Fu , Yang Xi-Bin , Long Gui-Yun , Jia Ze-Yan , Zeng Qing-Hui , Jin Dao-Chao , Yang Hong , Zhou Cao
Published: February 2022

The juvenile hormone (JH) is crucial for insect reproduction, and isopentenyl pyrophosphate isomerase (IPPI) is a key enzyme in the JH synthesis pathway. However, few studies have investigated how IPPI regulates insect reproduction. This study identifies and characterizes the IPPI gene (SfIPPI) from the important agricultural pest Sogatella furcifera. A phylogenetic analysis reveals a high homology of SfIPPI with the IPPI amino acid sequences of Laodelphax striatellus and Nilaparvata lugens (Stål). Furthermore, SfIPPI is expressed at various developmental stages and in various tissues of S. furcifera, and is significantly higher on the 5th day of adult emergence and in integument tissue, while lower levels are found on the 3rd day of adult emergence and in fat body and gut tissue. After silencing SfIPPI using RNA interference, the ovarian development is significantly inhibited and the fecundity is significantly reduced when compared with the control group. Additionally, SfIPPI silencing significantly decreases the expression levels of downstream JH signal transduction pathway genes (SfJHAMT, SfFAMeT, and SfKr-h1) and SfVg. Our findings are helpful in elucidating the molecular mechanism underlying the regulation of insect reproduction through genes in the JH synthesis pathway, and they provide a theoretical basis for the development of pest control treatments targeting SfIPPI.
Syndromic and Non-Syndromic Patients with Repaired Tetralogy of Fallot: Does It Affect the Long-Term Outcome?
Authors: Calcagni Giulio , Calvieri Camilla , Baban Anwar , Bianco Francesco , Barracano Rosaria , Caputo Massimo , Madrigali Andrea , Silva Kikina Stefani , Perrone Marco Alfonso , Digilio Maria Cristina , Pozzi Marco , Secinaro Aurelio , Sarubbi Berardo , Galletti Lorenzo , Gagliardi Maria Giulia , de Zorzi Andrea , Drago Fabrizio , Leonardi Benedetta
Published: February 2022

Background: The impact of genetic syndromes on cardiac magnetic resonance imaging (cMRI) parameters, particularly on right and/or left ventricular dysfunction, associated with clinical parameters following the repair of Tetralogy of Fallot (rToF) is not well known. Therefore, this study aimed to assess the differences in clinical, surgical, and cMRI data in syndromic and non-syndromic rToF patients. Methods: All syndromic rToF patients undergoing a cMRI without general anesthesia between 2010 and 2020 who were able to match with non-syndromic ones for birth date, sex, type of surgery, timing of cMRI, and BSA were selected. Demographic, clinical, surgical, MRI, ECG, and Holter ECG data were collected. Results: A total of one hundred and eight rToF patients equally subdivided into syndromic and non-syndromic, aged 18.7 ± 7.3 years, were studied. Del22q11.2 and Down syndrome (DS) were the most frequent syndromes (42.6% and 31.5%, respectively). Regarding the cMRI parameters considered, left ventricular (LV) dysfunction (LVEF < 50%) was more frequently found in syndromic patients (p = 0.040). In addition, they were older at repair (p = 0.002) but underwent earlier pulmonary valve replacement (PVR) (15.9 ± 5.6 vs. 19.5 ± 6.0 years, p = 0.049). On multivariate Cox regression analysis, adjusted for age at first repair, LV dysfunction remained significantly more associated with DS than del22q11.2 and non-syndromic patients (HR of 5.245; 95% CI 1.709–16.100, p = 0.004). There were only four episodes of non-sustained ventricular tachycardia in our cohort. Conclusions: Among the cMRI parameters commonly taken into consideration in rToF patients, LV dysfunction seemed to be the only one affected by the presence of a genetic syndrome. The percentage of patients performing PVR appears to be similar in both populations, although syndromic patients were older at repair and younger at PVR. Finally, the number of arrhythmic events in rToF patients seems to be low and unaffected by chromosomal abnormalities.
Ideal Depth of Endotracheal Intubation at the Vocal Cord Level in Pediatric Patients Considering Racial Differences in Tracheal Length
Authors: Yamamoto Tomohiro , Schindler Ehrenfried
Published: February 2022

Numerous formulas that can predict endotracheal intubation depth at the corner of the mouth or the nasal wing of patients have been reported, even though the oral and nasal cavity anatomies differ among patients. Therefore, the purpose of this study was to derive a simple and reliable formula to predict the ideal endotracheal tube insertion depth at the vocal cord level in pediatric patients. The current study was conducted as a retrospective observational study, involving 425 and 335 cardiac pediatric patients in Germany and Japan, respectively, and aimed to determine a formula for predicting tracheal length and ideal depth of endotracheal intubation at the vocal cord level in pediatric patients. The distance between the vocal cords and the carina tracheae was defined as the tracheal length, and was measured on preoperative chest radiographs obtained in the supine position. The tracheal length in cardiac pediatric patients ranged from 6 to 10% of the body height in Germany and from 7 to 11% in Japan. This study revealed racial differences in the tracheal length, that is, in the ideal depth of endotracheal intubation at the vocal cord level. This study suggests that an adequate endotracheal intubation depth can be achieved by inserting endotracheal tubes at the vocal cord level with the minimum tracheal length of each racial group in pediatric patients, for example, 6% and 7% of the body height in Europeans and Asians, respectively. If the endotracheal tube inserted with this method appears to be shallow on chest radiographs, this does not represent an increased risk of accidental extubation, due to an excessively short intubation depth, because the minimum tracheal length for each racial group is considered. That is, it is not due to the endotracheal tube insertion length, but is likely due to the tracheal length of the patient, who has a relatively long tracheal length in the racial group.
Multimodality Imaging in Cardiomyopathies with Hypertrophic Phenotypes
Authors: Monda Emanuele , Palmiero Giuseppe , Lioncino Michele , Rubino Marta , Cirillo Annapaola , Fusco Adelaide , Caiazza Martina , Verrillo Federica , Diana Gaetano , Mauriello Alfredo , Iavarone Michele , Losi Maria Angela , De Rimini Maria Luisa , Dellegrottaglie Santo , D’Andrea Antonello , Bossone Eduardo , Pacileo Giuseppe , Limongelli Giuseppe
Published: February 2022

Multimodality imaging is a comprehensive strategy to investigate left ventricular hypertrophy (LVH), providing morphologic, functional, and often clinical information to clinicians. Hypertrophic cardiomyopathy (HCM) is defined by an increased LV wall thickness not only explainable by abnormal loading conditions. In the context of HCM, multimodality imaging, by different imaging techniques, such as echocardiography, cardiac magnetic resonance, cardiac computer tomography, and cardiac nuclear imaging, provides essential information for diagnosis, sudden cardiac death stratification, and management. Furthermore, it is essential to uncover the specific cause of HCM, such as Fabry disease and cardiac amyloidosis, which can benefit of specific treatments. This review aims to elucidate the current role of multimodality imaging in adult patients with HCM.
Less-Intelligent and Unaware? Accuracy and Dunning–Kruger Effects for Self-Estimates of Different Aspects of Intelligence
Authors: Hofer Gabriela , Mraulak Valentina , Grinschgl Sandra , Neubauer Aljoscha C.
Published: February 2022

People’s perceptions of their intelligence correlate only moderately with objective intelligence measures. On average, people overestimate themselves. According to the popular Dunning–Kruger effect, this is particularly true for low performers: across many domains, those in the lowest quartile overestimate their abilities the most. However, recent work using improved statistical approaches found little support for a Dunning–Kruger effect in general intelligence. We investigated accuracy and Dunning–Kruger effects for self-estimates of general, verbal, numerical, and spatial intelligence—domains that differed in how well they can be judged in the past. A total of 281 participants completed self-estimates and intelligence measures online. Self-estimates showed mostly moderate correlational accuracy that was slightly higher for numerical intelligence and lower for verbal intelligence. Across domains, participants rated their intelligence as above average. However, as their intelligence was indeed high, this was not an overestimation. While standard analyses indicated Dunning–Kruger effects in general, verbal, and spatial intelligence, improved statistical methods only yielded some support for one in verbal intelligence: people with lower verbal intelligence tended to have less self-knowledge about it. The generalizability of these findings is limited to young, highly educated populations. Nevertheless, our results contribute to a growing literature questioning the generality of the Dunning–Kruger effect.
Length-Based Stock Assessment for the Data-Poor Bombay Duck Fishery from the Northern Bay of Bengal Coast, Bangladesh
Authors: Alam Mohammed Shahidul , Liu Qun , Schneider Petra , Mozumder Mohammad Mojibul Hoque , Chowdhury Mohammad Zahedur Rahman , Uddin Mohammad Muslem , Monwar Md. Mostafa , Hoque Md. Enamul , Barua Suman
Published: February 2022

The Bombay duck (Harpadon nehereus) forms the second-largest single-species marine fishery in Bangladesh and therefore has a significant impact on the local economy, providing employment, food, and nutrition to thousands of people. Despite the immense importance, this fishery has never been a priority for the relevant regulatory authorities. To enhance the sustainability of this fishery, an effective management policy based on the scientific evaluation of the current stock’s status is urgently required. Therefore, this study used three methodological approaches (traditional size structure-based stock assessment to reveal current exploitation status; the length-based spawning potential ratio (LB-SPR) to evaluate the stock’s spawning biomass; and Froese’s sustainability indicators for sustainable fishing) to conduct a thorough assessment of the Bombay duck stock to establish basic standards for the sustainable management of this fishery. The results revealed that this fishery is presently suffering from overexploitation and the stock’s spawning biomass (SPR = 8%) is below the limit reference point because of the juvenile-oriented fishing behavior of the fishery. Based on the outputs, this study recommended the mesh size regulation of the set bag nets (SBNs) (5 cm mesh size for the codend) to ensure not to catch immature fishes with a length equal to or smaller than 17.95 cm; and reduce the existing number of SBNs by half to reduce fishing pressure on the stock to ensure the sustainability of this fishery.
Effect of Carbon Sources on the Production of Volatile Organic Compounds by Fusarium verticillioides
Authors: Achimón Fernanda , Brito Vanessa D. , Pizzolitto Romina P. , Zygadlo Julio A.
Published: February 2022

The aim of the present study was to evaluate the effect of different carbon sources on the hydrocarbon-like volatile organic compounds (VOCs) of Fusarium verticillioides strain 7600 through a Principal Component Analysis approach, and to explore their diesel potential by using data from the literature. The fungus was cultivated in GYAM culture medium, and five carbon sources were evaluated: glucose, sucrose, xylose, lactose, and fructose. The VOCs were collected using a close-loop apparatus and identified through GC-MS. The same profile of 81 VOCs was detected with all treatments, but with different relative percentages among carbon sources. The production of branched-chain alkanes (30 compounds) ranged from 25.80% to 38.64%, straight-chain alkanes (12 compounds) from 22.04% to 24.18%, benzene derivatives (12 compounds) from 7.48% to 35.58%, and the biosynthesis of branched-chain alcohols (11 compounds) was from 6.82% to 16.71%, with lower values for the remaining groups of VOCs. Our results show that F. verticillioides has the metabolic potential to synthesize diesel-like VOCs. Further research should include the optimization of culture conditions other than carbon sources to increase the production of certain groups of VOCs.
Establishment of Adenomyosis Organoids as a Preclinical Model to Study Infertility
Authors: Juárez-Barber Elena , Francés-Herrero Emilio , Corachán Ana , Vidal Carmina , Giles Juan , Alamá Pilar , Faus Amparo , Pellicer Antonio , Cervelló Irene , Ferrero Hortensia
Published: February 2022

Adenomyosis is related to infertility and miscarriages, but so far there are no robust in vitro models that reproduce its pathological features to study the molecular mechanisms involved in this disease. Endometrial organoids are in vitro 3D models that recapitulate the native microenvironment and reproduce tissue characteristics that would allow the study of adenomyosis pathogenesis and related infertility disorders. In our study, human endometrial biopsies from adenomyosis (n = 6) and healthy women (n = 6) were recruited. Organoids were established and hormonally differentiated to recapitulate midsecretory and gestational endometrial phases. Physiological and pathological characteristics were evaluated by immunohistochemistry, immunofluorescence, qRT-PCR, and ELISA. Secretory and gestational organoids recapitulated in vivo glandular epithelial phenotype (pan-cytokeratin, Muc-1, PAS, Laminin, and Ki67) and secretory and gestational features (α-tubulin, SOX9, SPP1, PAEP, LIF, and 17βHSD2 expression and SPP1 secretion). Adenomyosis organoids showed higher expression of TGF-β2 and SMAD3 and increased gene expression of SPP1, PAEP, LIF, and 17βHSD2 compared with control organoids. Our results demonstrate that organoids derived from endometria of adenomyosis patients and differentiated to secretory and gestational phases recapitulate native endometrial-tissue-specific features and disease-specific traits. Adenomyosis-derived organoids are a promising in vitro preclinical model to study impaired implantation and pregnancy disorders in adenomyosis and enable personalized drug screening.
Effect of Bifidobacterium bifidum on Clinical Characteristics and Gut Microbiota in Attention-Deficit/Hyperactivity Disorder
Authors: Wang Liang-Jen , Yang Chia-Yu , Kuo Ho-Chang , Chou Wen-Jiun , Tsai Ching-Shu , Lee Sheng-Yu
Published: February 2022

This study aimed to examine whether probiotics supplements using Bifidobacterium bifidum (Bf-688) can improve clinical characteristics and gut microbiomes among patients with attention-deficit/hyperactivity disorder (ADHD). This open-label, single-arm trial consisted of 30 children aged 4–16 years who met the criteria for ADHD diagnosis. Each subject took Bf-688, with one sachet in the morning and one in the evening (daily bacteria count 5 × 109 CFUs), for 8 weeks. Patients’ clinical symptoms were assessed using the Swanson, Nolan, and Pelham Rating Scale (SNAP-IV). We collected stool samples at the baseline, the 8th week, and the 12th week for gut microbiota examination. During the 8-week Bf-688 supplement period, patients’ inattention symptoms and hyperactivity/impulsive symptoms improved, and their weights and BMIs increased. For gut microbiota, the Firmicutes to Bacteroidetes ratio (F/B ratio) decreased significantly. LEfSe analysis revealed that Firmicutes significantly decreased while Proteobacteria significantly increased during the 8-week treatment period. After Bf-688 was discontinued for 4 weeks (12 weeks from baseline), Bacteroidota significantly decreased and Shigella significantly increased. The probiotic Bf-688 supplement was associated with an improvement of clinical symptoms and with weight gain among ADHD children. Furthermore, gut microbiota composition was significantly altered by the Bf-688 supplement. A future randomized control trial is warranted to verify these findings.
Post-Resettlement Support Policies, Psychological Factors, and Farmers’ Homestead Exit Intention and Behavior
Authors: Shi Peng , Vanclay Frank , Yu Jin
Published: February 2022

Ex situ poverty alleviation relocation (ESPAR) is a Chinese government program created to address rural poverty and land management issues. Affecting around 10 million people, the process involves encouraging smallholder farmers to leave their rural homesteads and move to new housing. By consolidating people into medium density accommodation, the amount of land available for other purposes can be increased. However, some farmers were reluctant to move, and many of those who accepted new housing failed to demolish their homestead and rehabilitate the land as required. This paper applies the ‘extended theory of planned behavior’ to examine the factors that influence farmers’ exit intention and behavior. Face-to-face interviews were carried out in Southern Shaanxi Province, China. Data relating to 830 farmers were analyzed using Structural Equation Modelling. Our most important finding is that farmers had a poor perception of the post-relocation support, and this retarded their exit intention and behavior. We conclude that it is essential to improve post-relocation support, enhance the autonomy of farmers, and formulate relocation plans according to people’s age and likely future occupation.
A New Approach to Investigate the Spatially Heterogeneous in the Cooling Effects of Landscape Pattern
Authors: Liu Shuang , Li Xuefei , Chen Long , Zhao Qing , Zhao Chaohui , Hu Xisheng , Li Jian
Published: February 2022

Although many prior studies have found that landscape pattern significantly affects urban heat environment globally, the spatially heterogeneous in the cooling effects of landscape pattern remains poorly understood. In addition, most previous studies have only employed a single landscape metric separately, without holistic consideration of the composition and configuration of different landscapes. Taking one of the new “stove” cities in China-Fuzhou City, Fujian Province, as an example, we employed the principal component analysis (PCA) to synthesize a landscape pattern comprehensive index (LPCI) composed of the four common landscape metrics (i.e., aggregation index, AI; mean patch area, Area mn; largest patch index, LPI; and percentage of landscape, PLAND) of the three major land surfaces (i.e., water, vegetation, and impervious surface). Then, the local model (geographically weighted regression, GWR) was proposed to explore the spatially heterogeneous in the cooling effects of urban landscape. The results revealed that: (1) from 2000 to 2016, the land surface temperature (LST) increased by 4.262 °C, and the proportion of the urban heat island region showed an upward trend, while the urban-heat-island ratio index (URI) increased from 0.328 to 0.457; (2) the cooling effect of different land surfaces ranked from high to low was: water (29.69 °C), vegetation (38.56 °C), and impervious surface (41.82 °C); (3) compared with vegetation patches, water patches had a more obvious cooling effect on the surrounding environment, with the cooling distance within 60–90 m for the vegetation, while reaching 120–150 m for water body; (4) the proposed LPCI could explain more than 80% of the information for all of the landscape metrics for all of the landscape types, and presented a patchy distribution in the study area; (5) the GWR results revealed that the cooling effect of the landscape pattern varied spatially across the study area, indicating that the configuration of landscapes is more important in an urban center in alleviating urban heat environment than in an urban fringe area. The proposed approach provides a new understanding of the interaction between the landscape patterns and urban heat environments, providing a strong basis for landscape planning strategies for specific local sites.
The Structure and Evolution of the Tourism Economic Network of the Tibetan Plateau and Its Driving Factors
Authors: Chen Xiaodong , Wang Tian , Zheng Xin , Han Fang , Yang Zhaoping
Published: February 2022

Tourism is one of the core industries driving the economy on the Tibetan Plateau towards green development. Adopting the improved gravity model, social network analysis, and quadratic assignment procedure, this paper explores the structure and evolution characteristics of the tourism economic network on the Tibetan Plateau from 2015 to 2019. The results are as follows: (1) the tourism economic strength increased over time, but it was generally higher in Qinghai Province and lower in Tibet. (2) The network density exhibited an upwards trend overall. (3) The structure of the tourism economic network presented a significant core–periphery distribution pattern. Xining, Haixi, and Lhasa acted as a bridge between the spatial network structure of the tourism economy and had a structural hole advantage. However, some remote cities, such as Ali, Naqu, and Guoluo, had low network centricity. (4) The quadratic assignment procedure regression analysis showed that the A-level attractions and star-rated hotels significantly advanced the formation of the spatial association. This study concludes with recommendations for promoting tourism economic associations for policy-makers.
Income Change and Inter-Farmer Relations through Conservation Agriculture in Ishikawa Prefecture, Japan: Empirical Analysis of Economic and Behavioral Factors
Authors: Miyake Yoshitaka , Kimoto Shota , Uchiyama Yuta , Kohsaka Ryo
Published: February 2022

Conservation agriculture, also known as environment-friendly agriculture, is expected to contribute to global climate change mitigation and biodiversity conservation. To understand the effect of conservation agriculture on farmers and identify those factors, such as farmers’ income change, that might affect practices of conservation agriculture, perceptions, and output, this study examined farmers’ economic and behavioral factors, motivation, and satisfaction. We surveyed 51 farmers who are receiving subsidies to practice conservation agriculture in Ishikawa Prefecture, Japan. The survey is one of the first prefectural-scale studies that combines unique quantitative analysis of motivation and satisfaction levels (e.g., behaviors) in temporal sequence from the initial to current time to practice conservation agriculture. Our results showed that years of experience, trade with a retail shop, and the farmer’s age can affect income change. With regard to social factors, the satisfaction of their fellowship with other farmers practicing conservation agriculture was also significantly correlated with income change. Simultaneously, this category of satisfaction was difficult to attain compared to the other categories. Thus, greater effort is needed to enhance support networking among conservation farmers. Furthermore, the work presented here also provides the opportunity for future research on temporal and spatial questions surveying economic and behavioral effects with consideration of the heightened policy promotion and entrance of large retail industries.
Design of Amorphous Carbon Coatings Using Gaussian Processes and Advanced Data Visualization
Authors: Sauer Christopher , Rothammer Benedict , Pottin Nicolai , Bartz Marcel , Schleich Benjamin , Wartzack Sandro
Published: February 2022

In recent years, an increasing number of machine learning applications in tribology and coating design have been reported. Motivated by this, this contribution highlights the use of Gaussian processes for the prediction of the resulting coating characteristics to enhance the design of amorphous carbon coatings. In this regard, by using Gaussian process regression (GPR) models, a visualization of the process map of available coating design is created. The training of the GPR models is based on the experimental results of a centrally composed full factorial 23 experimental design for the deposition of a-C:H coatings on medical UHMWPE. In addition, different supervised machine learning (ML) models, such as Polynomial Regression (PR), Support Vector Machines (SVM) and Neural Networks (NN) are trained. All models are then used to predict the resulting indentation hardness of a complete statistical experimental design using the Box–Behnken design. The results are finally compared, with the GPR being of superior performance. The performance of the overall approach, in terms of quality and quantity of predictions as well as in terms of usage in visualization, is demonstrated using an initial dataset of 10 characterized amorphous carbon coatings on UHMWPE.