bluuebunny/crossref_metadata_2025 huggingface dataset:
Advanced Scheme to Generate MHz, Fully Coherent FEL Pulses at nm Wavelength
Authors: Paraskaki Georgia , Ackermann Sven , Faatz Bart , Geloni Gianluca , Lang Tino , Pannek Fabian , Schaper Lucas , Zemella Johann
Published: June 2021

Current FEL development efforts aim at improving the control of coherence at high repetition rate while keeping the wavelength tunability. Seeding schemes, like HGHG and EEHG, allow for the generation of fully coherent FEL pulses, but the powerful external seed laser required limits the repetition rate that can be achieved. In turn, this impacts the average brightness and the amount of statistics that experiments can do. In order to solve this issue, here we take a unique approach and discuss the use of one or more optical cavities to seed the electron bunches accelerated in a superconducting linac to modulate their energy. Like standard seeding schemes, the cavity is followed by a dispersive section, which manipulates the longitudinal phase space of the electron bunches, inducing longitudinal density modulations with high harmonic content that undergo the FEL process in an amplifier placed downstream. We will discuss technical requirements for implementing these setups and their operation range based on numerical simulations.
Evaluation of Deep Learning against Conventional Limit Equilibrium Methods for Slope Stability Analysis
Authors: Azmoon Behnam , Biniyaz Aynaz , Liu Zhen (Leo)
Published: June 2021

This paper presents a comparison study between methods of deep learning as a new category of slope stability analysis, built upon the recent advances in artificial intelligence and conventional limit equilibrium analysis methods. For this purpose, computer code was developed to calculate the factor of safety (FS) using four limit equilibrium methods: Bishop’s simplified method, the Fellenius method, Janbu’s simplified method, and Janbu’s corrected method. The code was verified against Slide2 in RocScience. Subsequently, the average FS values were used to approximate the “true” FS of the slopes for labeling the images for deep learning. Using this code, a comprehensive dataset of slope images with wide ranges of geometries and soil properties was created. The average FS values were used to label the images for implementing two deep learning models: a multiclass classification and a regression model. After training, the deep learning models were used to predict the FS of an independent set of slope images. Finally, the performance of the models was compared to that of the conventional methods. This study found that deep learning methods can reach accuracies as high as 99.71% while improving computational efficiency by more than 18 times compared with conventional methods.
Characteristics of Energy Dissipation Rate Observed from the High-Frequency Sonic Anemometer at Boseong, South Korea
Authors: Kim Jeonghoe , Kim Jung-Hoon , Sharman Robert D.
Published: June 2021

The characteristics of low-level turbulence at Boseong, located on the southern coast of South Korea, were investigated in terms of eddy dissipation rate (EDR) using 1-year (2018) of wind data obtained from the Boseong Meteorological Observatory (BMO), a World Meteorological Organization testbed. At BMO, a 307 m tall tower is installed on which four high-frequency (20 Hz) sonic anemometers are mounted at 60, 140, and 300 m above ground level (AGL). In addition, a sonic anemometer at 2.5 m AGL is located to the south of the tower. EDRs are estimated from the wind measurements based on three different EDR estimation methods. The first two methods use the inertial dissipation method derived from Kolmogorov turbulence theory, and the third uses a maximum likelihood estimation assuming a von Kármán spectral model. Reasonable agreement was obtained between the three methods with various fluctuations, including diurnal variations for all seasons, while the EDR calculated from the third method displayed slightly higher EDR values than the other two methods. The result of the analysis showed that the mean (standard deviations) of logarithms of EDR had larger values as height decreased (increased), and the means were higher in the unstable planetary boundary layer (PBL) than in the stable PBL for this heterogeneous location adjacent to the coastlines. The probability density functions (PDFs) of the EDRs showed that the distribution was well-represented by a lognormal distribution in both the stable and unstable PBL, although the PDFs at the lowest level (2.5 m) deviated from those at other levels due to surface effects. Seasonal variations in the PDFs showed that there was less difference in the shape of the PDFs depending on atmospheric stability in the wintertime. Finally, we calculate the 1-yr statistics of the observed EDR, which will be used for future LLT forecast systems in Korea.
Possible Associations between the Number of Cold Days over East Asia and Arctic Oscillation and Arctic Warming
Authors: Song Wei , Ye Xiaochen
Published: June 2021

Based on the NECP/NCAR reanalysis dataset, the associations between the number of cold days (NCD) over East Asia (100–150° E, 25–55° N) and Arctic Oscillation (AO)/Arctic warming during 1956–2015 are explored. The results show the NCD was closely associated with AO during 1956–1990 and Arctic warming during 1991–2015. It reveals NCD over East Asia showed a downward trend and a significantly negative correlation with AO in the previous stage, while it presented an upward trend and notably positive association with Arctic warming in the later period. Meanwhile the increase in the earlier-stage AO will often be accompanied by the weakness of the Siberian high (SH), the Ural Mountains Blocking high (UBH), and the East Asian trough (EAT), and a “positive–negative–positive” wave band exist in the upper troposphere, which is linked with weakened northerly wind over East Asia. All these anomalies are unfavorable for the southward transportation of cold air, eventually leading to the decrease in NCD over East Asia. Additionally, when the near-surface temperature over the Arctic rises in the later period, on the one hand, SH reinforces and further results in more NCD over East Asia; on the other hand, the 1000–500 hPa thickness field displays a “north positive–south negative” pattern, which is conducive to the deceleration of the westerlies at mid-latitudes over Eurasia, and further bring about the enhancement of EAT and UBH, favoring the southward intrusion of cold air, finally, more NCD are generated.
Analysis of Ozone Pollution Characteristics and Influencing Factors in Northeast Economic Cooperation Region, China
Authors: Tian Jiaqi , Fang Chunsheng , Qiu Jiaxin , Wang Ju
Published: June 2021

The increase in tropospheric ozone (O3) concentration has become one of the factors restricting urban development. This paper selected the important economic cooperation areas in Northeast China as the research object and collected the hourly monitoring data of pollutants and meteorological data in 11 cities from 1 January 2015 to 31 December 2019. The temporal and spatial variation trend of O3 concentration and the effects of meteorological factors and other pollutants, including CO (carbon monoxide), SO2 (sulfur dioxide), NO2 (nitrogen dioxide), and PM2.5 and PM10 (PM particles with aerodynamic diameters less than 2.5 μm and 10 μm) on ozone concentration were analyzed. At the same time, the variation period of O3 concentration was further analyzed by Morlet wavelet analysis. The results showed that the O3 pollution in the study area had a significant spatial correlation. The spatial distribution showed that the O3 concentration was relatively high in the south and low in the northeast. Seasonally, the O3 concentration was the highest in spring, followed by summer, and the lowest in winter. The diurnal variation of O3 concentration presented a “single peak” pattern. O3 concentration had a significant positive correlation with temperature, sunshine duration, and wind speed and a significant anticorrelation with CO, NO2, SO2, and PM2.5 concentration. Under the time scale of a = 9, 23, O3 had significant periodic fluctuation, which was similar to those of wind speed and temperature.
Spatiotemporal Characteristics of Drought in the North China Plain over the Past 58 Years
Authors: Cui Yanqiang , Zhang Bo , Huang Hao , Zeng Jianjun , Wang Xiaodan , Jiao Wenhui
Published: June 2021

Understanding the spatiotemporal characteristics of regional drought is of great significance in decision-making processes such as water resources and agricultural systems management. The North China Plain is an important grain production base in China and the most drought-prone region in the country. In this study, the monthly standardized precipitation evapotranspiration index (SPEI) was used to monitor the spatiotemporal variation of agricultural drought in the North China Plain from 1960 to 2017. Seven spatial patterns of drought variability were identified in the North China Plain, such as Huang-Huai Plain, Lower Yangtze River Plain, Haihe Plain, Shandong Hills, Qinling Mountains Margin area, Huangshan Mountain surroundings, and Yanshan Mountain margin area. The spatial models showed different trends in different time stages, indicating that the drought conditions in the North China Plain were complex and changeable in the past 58 years. As an important agricultural area, the North China Plain needs more attention since this region shows a remarkable trend of drought and, as such, will definitely increase the water demand for agricultural irrigation. The strong correlation between these spatial distribution patterns indicates that the climate and weather conditions leading to drought are consistent and that drought conditions are independent for regions that are not correlated. If this trend continues, the characteristics of drought variability in the North China Plain will become more complex, and a more detailed water management strategy will be needed to address the effects of drought on agro-ecosystems. Recognizing the drought variability in the North China Plain can provide a basis for agricultural disaster reduction planning and water resources allocation.
Analysis of Multipolar Linear Paul Traps for Ion–Atom Ultracold Collision Experiments
Authors: Niranjan M. , Prakash Anand , Rangwala S. A.
Published: June 2021

We evaluate the performance of multipole, linear Paul traps for the purpose of studying cold ion–atom collisions. A combination of numerical simulations and analysis based on the virial theorem is used to draw conclusions on the differences that result, by considering the trapping details of several multipole trap types. Starting with an analysis of how a low energy collision takes place between a fully compensated, ultracold trapped ion and an stationary atom, we show that a higher order multipole trap is, in principle, advantageous in terms of collisional heating. The virial analysis of multipole traps then follows, along with the computation of trapped ion trajectories in the quadrupole, hexapole, octopole and do-decapole radio frequency traps. A detailed analysis of the motion of trapped ions as a function of the amplitude, phase and stability of the ion’s motion is used to evaluate the experimental prospects for such traps. The present analysis has the virtue of providing definitive answers for the merits of the various configurations, using first principles.
Ancestry Studies in Forensic Anthropology: Back on the Frontier of Racism
Authors: Ross Ann H. , Williams Shanna E.
Published: June 2021

One of the parameters forensic anthropologists have traditionally estimated is ancestry, which is used in the United States as a proxy for social race. Its use is controversial because the biological race concept was debunked by scientists decades ago. However, many forensic anthropologists contend, in part, that because social race categories used by law enforcement can be predicted by cranial variation, ancestry remains a necessary parameter for estimation. Here, we use content analysis of the Journal of Forensic Sciences for the period 2009–2019 to demonstrate the use of various nomenclature and resultant confusion in ancestry estimation studies, and as a mechanism to discuss how forensic anthropologists have eschewed a human variation approach to studying human morphological differences in favor of a simplistic and debunked typological one. Further, we employ modern geometric morphometric and spatial analysis methods on craniofacial coordinate anatomical landmarks from several Latin American samples to test the validity of applying the antiquated tri-continental approach to ancestry (i.e., African, Asian, European). Our results indicate groups are not patterned by the ancestry trifecta. These findings illustrate the benefit and necessity of embracing studies that employ population structure models to better understand human variation and the historical factors that have influenced it.
Secondary Structure of the Novel Myosin Binding Domain WYR and Implications within Myosin Structure
Authors: Menard Lynda M. , Wood Neil B. , Vigoreaux Jim O.
Published: June 2021

Structural changes in the myosin II light meromyosin (LMM) that influence thick filament mechanical properties and muscle function are modulated by LMM-binding proteins. Flightin is an LMM-binding protein indispensable for the function of Drosophila indirect flight muscle (IFM). Flightin has a three-domain structure that includes WYR, a novel 52 aa domain conserved throughout Pancrustacea. In this study, we (i) test the hypothesis that WYR binds the LMM, (ii) characterize the secondary structure of WYR, and (iii) examine the structural impact WYR has on the LMM. Circular dichroism at 260–190 nm reveals a structural profile for WYR and supports an interaction between WYR and LMM. A WYR–LMM interaction is supported by co-sedimentation with a stoichiometry of ~2.4:1. The WYR–LMM interaction results in an overall increased coiled-coil content, while curtailing ɑ helical content. WYR is found to be composed of 15% turns, 31% antiparallel β, and 48% ‘other’ content. We propose a structural model of WYR consisting of an antiparallel β hairpin between Q92-K114 centered on an ASX or β turn around N102, with a G1 bulge at G117. The Drosophila LMM segment used, V1346-I1941, encompassing conserved skip residues 2-4, is found to possess a traditional helical profile but is interpreted as having <30% helical content by multiple methods of deconvolution. This low helicity may be affiliated with the dynamic behavior of the structure in solution or the inclusion of a known non-helical region in the C-terminus. Our results support the hypothesis that WYR binds the LMM and that this interaction brings about structural changes in the coiled-coil. These studies implicate flightin, via the WYR domain, for distinct shifts in LMM secondary structure that could influence the structural properties and stabilization of the thick filament, scaling to modulation of whole muscle function.
Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications
Authors: Saad Mohd Faiz Mat , Sau Aziz Ramlee , Akbar Muhamad Afiq , Baharum Syarul Nataqain , Ramzi Ahmad Bazli , Talip Noraini , Bunawan Hamidun
Published: June 2021

Begomovirus has become a potential threat to the agriculture sector. It causes significant losses to several economically important crops. Given this considerable loss, the development of tools to study viral genomes and function is needed. Infectious clones approaches and applications have allowed the direct exploitation of virus genomes. Infectious clones of DNA viruses are the critical instrument for functional characterization of the notable and newly discovered virus. Understanding of structure and composition of viruses has contributed to the evolution of molecular plant pathology. Therefore, this review provides extensive guidelines on the strategy to construct infectious clones of Begomovirus. Also, this technique’s impacts and benefits in controlling and understanding the Begomovirus infection will be discussed.
Meroterpenoids: A Comprehensive Update Insight on Structural Diversity and Biology
Authors: Nazir Mamona , Saleem Muhammad , Tousif Muhammad Imran , Anwar Muhammad Aijaz , Surup Frank , Ali Iftikhar , Wang Daijie , Mamadalieva Nilufar Z. , Alshammari Elham , Ashour Mohamed L. , Ashour Ahmed M. , Ahmed Ishtiaq , Elizbit  , Green Ivan R. , Hussain Hidayat
Published: June 2021

Meroterpenoids are secondary metabolites formed due to mixed biosynthetic pathways which are produced in part from a terpenoid co-substrate. These mixed biosynthetically hybrid compounds are widely produced by bacteria, algae, plants, and animals. Notably amazing chemical diversity is generated among meroterpenoids via a combination of terpenoid scaffolds with polyketides, alkaloids, phenols, and amino acids. This review deals with the isolation, chemical diversity, and biological effects of 452 new meroterpenoids reported from natural sources from January 2016 to December 2020. Most of the meroterpenoids possess antimicrobial, cytotoxic, antioxidant, anti-inflammatory, antiviral, enzyme inhibitory, and immunosupressive effects.
Long-Term Tri-Modal In Vivo Tracking of Engrafted Cartilage-Derived Stem/Progenitor Cells Based on Upconversion Nanoparticles
Authors: Chen Chu-Hsin , Tang Na , Xue Ke , Zhang Hui-Zhong , Chen Ya-Hong , Xu Peng , Sun Kang , Tao Ke , Liu Kai
Published: June 2021

Cartilage-derived stem/progenitor cells (CSPCs) are a potential choice for seed cells in osteal and chondral regeneration, and the outcomes of their survival and position distribution in vivo form the basis for the investigation of their mechanism. However, the current use of in vivo stem cell tracing techniques in laboratories is relatively limited, owing to their high operating costs and cytotoxicity. Herein, we performed tri-modal in vivo imaging of CSPCs during subcutaneous chondrogenesis using upconversion nanoparticles (UCNPs) for 28 days. Distinctive signals at accurate positions were acquired without signal noise from X-ray computed tomography, magnetic resonance imaging, and upconversion luminescence. The measured intensities were all significantly proportional to the cell numbers, thereby enabling real-time in vivo quantification of the implanted cells. However, limitations of the detectable range of cell numbers were also observed, owing to the imaging shortcomings of UCNPs, which requires further improvement of the nanoparticles. Our study explores the application value of upconversion nanomaterials in the tri-modal monitoring of implanted stem cells and provides new perspectives for future clinical translation.
Copper Imbalance in Alzheimer’s Disease: Meta-Analysis of Serum, Plasma, and Brain Specimens, and Replication Study Evaluating ATP7B Gene Variants
Authors: Squitti Rosanna , Ventriglia Mariacarla , Simonelli Ilaria , Bonvicini Cristian , Costa Alfredo , Perini Giulia , Binetti Giuliano , Benussi Luisa , Ghidoni Roberta , Koch Giacomo , Borroni Barbara , Albanese Alberto , Sensi Stefano L. , Rongioletti Mauro
Published: June 2021

Evidence indicates that patients with Alzheimer’s dementia (AD) show signs of copper (Cu) dyshomeostasis. This study aimed at evaluating the potential of Cu dysregulation as an AD susceptibility factor. We performed a meta-analysis of 56 studies investigating Cu biomarkers in brain specimens (pooled total of 182 AD and 166 healthy controls, HC) and in serum/plasma (pooled total of 2929 AD and 3547 HC). We also completed a replication study of serum Cu biomarkers in 97 AD patients and 70 HC screened for rs732774 and rs1061472 ATP7B, the gene encoding for the Cu transporter ATPase7B. Our meta-analysis showed decreased Cu in AD brain specimens, increased Cu and nonbound ceruloplasmin (Non-Cp) Cu in serum/plasma samples, and unchanged ceruloplasmin. Serum/plasma Cu excess was associated with a three to fourfold increase in the risk of having AD. Our replication study confirmed meta-analysis results and showed that carriers of the ATP7B AG haplotype were significantly more frequent in the AD group. Overall, our study shows that AD patients fail to maintain a Cu metabolic balance and reveals the presence of a percentage of AD patients carrying ATP7B AG haplotype and presenting Non-Cp Cu excess, which suggest that a subset of AD subjects is prone to Cu imbalance. This AD subtype can be the target of precision medicine-based strategies tackling Cu dysregulation.
Circulating Extracellular miRNA Analysis in Patients with Stable CAD and Acute Coronary Syndromes
Authors: Zhelankin Andrey V. , Stonogina Daria A. , Vasiliev Sergey V. , Babalyan Konstantin A. , Sharova Elena I. , Doludin Yurii V. , Shchekochikhin Dmitry Y. , Generozov Eduard V. , Akselrod Anna S.
Published: June 2021

Extracellular circulating microRNAs (miRNAs) are currently a focus of interest as non-invasive biomarkers of cardiovascular pathologies, including coronary artery disease (CAD) and acute coronary syndromes (ACS): myocardial infarction with and without ST-segment elevation (STEMI and NSTEMI) and unstable angina (UA). However, the current data for some miRNAs are controversial and inconsistent, probably due to pre-analytical and methodological variances in different studies. In this work, we fulfilled the basic pre-analytical requirements provided for circulating miRNA studies for application to stable CAD and ACS research. We used quantitative PCR to determine the relative plasma levels of eight circulating miRNAs that are potentially associated with atherosclerosis. In a cohort of 136 adult clinic CAD patients and outpatient controls, we found that the plasma levels of miR-21-5p and miR-146a-5p were significantly elevated in ACS patients, and the level of miR-17-5p was decreased in ACS and stable CAD patients compared to both healthy controls and hypertensive patients without CAD. Within the ACS patient group, no differences were found in the plasma levels of these miRNAs between patients with positive and negative troponin, nor were any differences found between STEMI and NSTEMI. Our results indicate that increased plasma levels of miR-146a-5p and miR-21-5p can be considered general ACS circulating biomarkers and that lowered miR-17-5p can be considered a general biomarker of CAD.
Proof-of-Concept for the Analgesic Effect and Thermoregulatory Safety of Orally Administered Multi-Target Compound SZV 1287 in Mice: A Novel Drug Candidate for Neuropathic Pain
Authors: Horváth Ádám István , Szentes Nikolett , Tékus Valéria , Payrits Maja , Szőke Éva , Oláh Emőke , Garami András , Fliszár-Nyúl Eszter , Poór Miklós , Sár Cecília , Kálai Tamás , Pál Szilárd , Percze Krisztina , Scholz Éva Nagyné , Mészáros Tamás , Tóth Blanka , Mátyus Péter , Helyes Zsuzsanna
Published: June 2021

SZV 1287 (3-(4,5-diphenyl-1,3-oxazol-2-yl)propanal oxime) is a novel multi-target candidate under preclinical development for neuropathic pain. It inhibits amine oxidase copper containing 3, transient receptor potential ankyrin 1 and vanilloid 1 (TRPV1) receptors. Mainly under acidic conditions, it is transformed to the cyclooxygenase inhibitor oxaprozin, which is ineffective for neuropathy. Therefore, an enterosolvent capsule is suggested for oral formulation, which we investigated for nociception, basic kinetics, and thermoregulatory safety in mice. The antihyperalgesic effect of SZV 1287 (10, 20, 50, and 200 mg/kg, p.o.) was determined in partial sciatic nerve ligation-induced traumatic neuropathy by aesthesiometry, brain and plasma concentrations by HPLC, and deep body temperature by thermometry. Its effect on proton-induced TRPV1 activation involved in thermoregulation was assessed by microfluorimetry in cultured trigeminal neurons. The three higher SZV 1287 doses significantly, but not dose-dependently, reduced neuropathic hyperalgesia by 50% of its maximal effect. It was quickly absorbed; plasma concentration was stable for 2 h, and it entered into the brain. Although SZV 1287 significantly decreased the proton-induced TRPV1-mediated calcium-influx potentially leading to hyperthermia, it did not alter deep body temperature. Oral SZV 1287 inhibited neuropathic hyperalgesia and, despite TRPV1 antagonistic action and brain penetration, it did not influence thermoregulation, which makes it a promising analgesic candidate.
Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis
Authors: Budvytyte Rima , Milasiute Akvile , Vitkus Dalius , Strupas Kestutis , Gulla Aiste , Sakinyte Ieva , Razumiene Julija
Published: June 2021

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.
The Association between Tau Protein Level in Cerebrospinal Fluid and Cognitive Status: A Large-Scale Analysis of GAAIN Database
Authors: Eckhoff Kyle , Morris Robert , Zuluaga Valeria , Polsky Rebecca , Cheng Feng
Published: June 2021

Alzheimer’s disease (AD) and the associated neurodegenerative dementia have become of increasing concern in healthcare. The tau protein has been considered a key hallmark of progressive neurodegeneration. In this paper, a large-scale analysis of five datasets (more than 2500 people) from the Global Alzheimer’s Association Interactive Network (GAAIN) databases was performed to investigate the association between the level of tau protein, including total tau and phosphorylated tau (p-tau), in cerebrospinal fluid (CSF) and cognitive status. Statistically significant (or marginally significant) high total tau or p-tau concentrations in CSF were observed in dementia patients compared with healthy people in all datasets. There is also a statistically significant (or marginally significant) negative correlation between p-tau concentrations in CSF and Folstein Mini-Mental State Examination (MMSE) scores. In addition, transcriptomic data derived from mouse microglial cells showed multiple genes upregulated in Toll-like receptor signaling and Alzheimer’s disease pathways, including TNF, TLR2, IL-1β, and COX subunits, suggesting that the mechanism of action that relates p-tau and MMSE scores may be through overactivation of pro-inflammatory microglial activity by Aβ peptides, TNF-mediated hyperphosphorylation of tau, and the infectious spread of pathological tau across healthy neurons. Our results not only confirmed the association between tau protein level and cognitive status in a large population but also provided useful information for the understanding of the role of tau in neurodegeneration and the development of dementia.
Implications of Circadian Rhythm in Stroke Occurrence: Certainties and Possibilities
Authors: Fodor Dana Marieta , Marta Monica Mihaela , Perju-Dumbravă Lăcrămioara
Published: June 2021

Stroke occurrence is not randomly distributed over time but has circadian rhythmicity with the highest frequency of onset in the morning hours. This specific temporal pattern is valid for all subtypes of cerebral infarction and intracerebral hemorrhage. It also correlates with the circadian variation of some exogenous factors such as orthostatic changes, physical activity, sleep-awake cycle, as well as with endogenous factors including dipping patterns of blood pressure, or morning prothrombotic and hypofibrinolytic states with underlying cyclic changes in the autonomous system and humoral activity. Since the internal clock is responsible for these circadian biological changes, its disruption may increase the risk of stroke occurrence and influence neuronal susceptibility to injury and neurorehabilitation. This review aims to summarize the literature data on the circadian variation of cerebrovascular events according to physiological, cellular, and molecular circadian changes, to survey the available information on the chronotherapy and chronoprophylaxis of stroke and its risk factors, as well as to discuss the less reviewed impact of the circadian rhythm in stroke onset on patient outcome and functional status after stroke.
Editorial Catalysts: Supported Metal Catalysts and Their Applications in Fine Chemicals
Authors: Evangelisti Claudio , Mandoli Alessandro
Published: June 2021

Heterogeneous catalysis is an essential tool for the development of both emerging and established chemical processes, as well as for their economic and environmental sustainability [...]
WO3 Fibers/g-C3N4 Z-Scheme Heterostructure Photocatalysts for Simultaneous Oxidation/Reduction of Phenol/Cr (VI) in Aquatic Media
Authors: Bairamis Feidias , Konstantinou Ioannis
Published: June 2021

A sequence of WO3/g-C3N4 composites was synthesized at various % weight ratios (1, 5, 6.5, 8, 10, and 15%) of WO3 into g-C3N4 via electrospinning and wet-mixing method. The prepared photocatalytic materials were characterized by X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, UV–vis diffuse reflection spectroscopy (DRS), scanning electron microscopy (SEM), N2 porosimetry and dynamic light scattering (DLS). Electrospun fibers of WO3 with diameter 250–300 nm was prepared using polyvinylpyrrolidone (PVP) polymer and used for the synthesis of composite WO3/g-C3N4 heterojunction structures. Results showed mesoporous materials with triclinic WO3 crystal phase, surface areas up to 67.7 m2g−1 and band gaps lower than 2.5 eV confirming the absorption to visible light region. The photocatalytic performance of the prepared photocatalysts were assessed towards the oxidation of phenol and reduction of Cr (VI), in single and binary systems using simulated solar light illumination, that followed first-order kinetics. The WO3/g-C3N4 composites were found to exhibit improved photocatalytic performances compared to the pure WO3 and g-C3N4 with 6.5 wt% WO3/g-C3N4 and 5 wt% WO3/g-C3N4 composites being the most efficient catalysts for the oxidation of phenolics and reduction of Cr (VI), respectively. The improved performance was explained by a Z-scheme photocatalytic mechanism which was proposed based on scavenging experiments and the determination of the corresponding energy levels of valence and conduction bands. The study demonstrated that such composites present interesting photocatalytic properties that can be further expanded to other environmental depollution applications as well as in energy applications.
Application of Floating TiO2 Photocatalyst for Methylene Blue Decomposition and Salmonella typhimurium Inactivation
Authors: Urbonavicius Marius , Varnagiris Sarunas , Sakalauskaite Sandra , Demikyte Emilija , Tuckute Simona , Lelis Martynas
Published: June 2021

The growing level of wastewater as well as pollution of freshwater by various bacteria are essential worldwide issues which have to be solved. In this contribution, nanocrystalline anatase TiO2 films deposited by magnetron sputtering on high-density polystyrene (HDPE) beads were applied as floating photocatalysts for Salmonella typhimurium bacterial inactivation in water for the first time. Additionally, the photocatalytic degradation of methylene blue dye in the presence of HDPE beads with TiO2 film under UV-B irradiation was investigated. The suitability to adopt such floating photocatalyst structures for practical applications was tested in cycling experiments. The detailed surface morphology, crystal structure, elemental mapping, surface chemical composition and bond analysis of deposited TiO2 films were investigated by scanning electron microscope, X-ray diffractometer and X-ray photoelectron spectroscope techniques. The bacterial viability as well as MB decomposition experiments showed promising results by demonstrating that 6% of bacterial colonies were formed after the first run and only about 1% after the next four runs, which is an appropriate outcome for practical applications. NPN uptake results showed that the permeability of the outer membrane was significantly increased as well.
Angiogenic Properties of NK Cells in Cancer and Other Angiogenesis-Dependent Diseases
Authors: Radomska-Leśniewska Dorota M. , Białoszewska Agata , Kamiński Paweł
Published: June 2021

The pathogenesis of many serious diseases, including cancer, is closely related to disturbances in the angiogenesis process. Angiogenesis is essential for the progression of tumor growth and metastasis. The tumor microenvironment (TME) has immunosuppressive properties, which contribute to tumor expansion and angiogenesis. Similarly, the uterine microenvironment (UME) exerts a tolerogenic (immunosuppressive) and proangiogenic effect on its cells, promoting implantation and development of the embryo and placenta. In the TME and UME natural killer (NK) cells, which otherwise are capable of killing target cells autonomously, enter a state of reduced cytotoxicity or anergy. Both TME and UME are rich with factors (e.g., TGF-β, glycodelin, hypoxia), which support a conversion of NK cells to the low/non-cytotoxic, proangiogenic CD56brightCD16low phenotype. It is plausible that the phenomenon of acquiring proangiogenic and low cytotoxic features by NK cells is not only limited to cancer but is a common feature of different angiogenesis-dependent diseases (ADDs). In this review, we will discuss the role of NK cells in angiogenesis disturbances associated with cancer and other selected ADDs. Expanding the knowledge of the mechanisms responsible for angiogenesis and its disorders contributes to a better understanding of ADDs and may have therapeutic implications.
Polish Medical Air Rescue Crew Interventions Concerning Neonatal Patients
Authors: Rzońca Ewa , Bączek Grażyna , Podgórski Marcin , Gałązkowski Robert
Published: June 2021

The purpose of the study was to present the characteristics of Helicopter Emergency Medical Service (HEMS) and Emergency Medical Service (EMS) interventions concerning newborns in Poland. The study involved a retrospective analysis of missions by Polish Medical Air Rescue crews concerning newborns, carried out in Poland between January 2011 and December 2020. Polish Medical Air Rescue crews were most commonly dispatched to urban areas (86.83%), for patient transfer (59.67%), using an airplane (65.43%), between 7 AM and 6:59 PM (93.14%), and in the summer (28.67%). Further management involved handing over the neonatal patient to a ground neonatal ambulance team. Most of the patients studied were male (58.02%), and the most common diagnosis requiring the HEMS or EMS intervention was a congenital heart defect (31.41%). The most common medical emergency procedure performed by Polish Medical Air Rescue crew members for the neonatal patients was intravenous cannulation (43.07%). The odds ratio for congenital malformations was higher in male newborns. The type of Polish Medical Air Rescue mission was associated with the location of the call, time of the call, ICD-10 diagnosis associated with the dispatch, selected clinical findings, most commonly performed medical emergency procedures, and mission duration and distance covered.
Care Coordination Needs of Families of Children with Down Syndrome: A Scoping Review to Inform Development of mHealth Applications for Families
Authors: Skelton Beth , Knafl Kathleen , Van Riper Marcia , Fleming Louise , Swallow Veronica
Published: June 2021

Care coordination is a critical component of health management aimed at linking care providers and health-information-involved care management. Our intent in this scoping review was to identify care coordination needs of families of children with Down syndrome (DS) and the strategies they used to meet those needs, with the goal of contributing to the evidence base for developing interventions by using an mHealth application (mHealth apps) for these families. Using established guidelines for scoping reviews, we searched five databases, yielding 2149 articles. Following abstract and full-text review, we identified 38 articles meeting our inclusion criteria. Studies incorporated varied in regard to research designs, samples, measures, and analytic approaches, with only one testing an intervention by using mHealth apps. Across studies, data came from 4882 families. Common aspects of families’ care coordination needs included communication and information needs and utilization of healthcare resources. Additional themes were identified related to individual, family, and healthcare contextual factors. Authors also reported families’ recommendations for desirable characteristics of an mHealth apps that addressed the design of a personal health record, meeting age-specific information needs, and ensuring access to up-to-date information. These results will further the development of mHealth apps that are tailored to the needs of families with a child with DS.
Long-Term Properties of Different Fiber Reinforcement Effect on Fly Ash-Based Geopolymer Composite
Authors: Gailitis Rihards , Sprince Andina , Kozlovskis Tomass , Radina Liga , Pakrastins Leonids , Vatin Nikolai
Published: June 2021

Geopolymer composites have been around only for 40 years. Nowadays, they are used in buildings and infrastructures of various kinds. A geopolymer’s main benefit is that it is a green material that is partially made by utilizing waste products. The carbon footprint from geopolymer matrix manufacturing is at least two times less than Portland cement manufacturing. Due to the nature of the geopolymer manufacturing process, there is a high risk of shrinkage that could develop unwanted micro-cracks that could reduce strength and create higher creep strains. Because of this concern, a common strategy to reduce long-term strains of the material, such as shrinkage and creep, is to add fiber reinforcement that would constrain crack development in the material. This article aims to determine how various kinds and amounts of different fiber reinforcement affect fly ash-based geopolymer composites’ creep strains in compression. Specimen mixes were produced with 1% steel fibers, 1% polypropylene fibers, 5% polypropylene fibers, and without fibers (plain geopolymer). For creep and shrinkage testing, cylindrical specimens Ø46 × 190 mm were used. The highest creep resistance was observed in 5% polypropylene fiber specimens, followed by 1% polypropylene fiber, plain, and 1% steel fiber specimens. The highest compressive strength was observed in 1% polypropylene fiber specimens, followed by plain specimens, 1% steel fiber specimens, and 5% polypropylene fiber-reinforced specimens. The only fiber-reinforced geopolymer mix with improved long-term properties was observed with 1% polypropylene fiber inclusion, whereas other fiber-introduced mixes showed significant decreases in long-term properties. The geopolymer composite mix with 1% polypropylene fiber reinforcement showed a reduction in creep strains of 31% compared to the plain geopolymer composite.
Determination of Non-Invasive Biomarkers for the Assessment of Fibrosis, Steatosis and Hepatic Iron Overload by MR Image Analysis. A Pilot Study
Authors: Meneses Alba , Santabárbara José Manuel , Romero Juan Antonio , Aliaga Roberto , Maceira Alicia María , Moratal David
Published: June 2021

The reference diagnostic test of fibrosis, steatosis, and hepatic iron overload is liver biopsy, a clear invasive procedure. The main objective of this work was to propose HSA, or human serum albumin, as a biomarker for the assessment of fibrosis and to study non-invasive biomarkers for the assessment of steatosis and hepatic iron overload by means of an MR image acquisition protocol. It was performed on a set of eight subjects to determine fibrosis, steatosis, and hepatic iron overload with four different MRI sequences. We calibrated longitudinal relaxation times (T1 [ms]) with seven human serum albumin (HSA [%]) phantoms, and we studied the relationship between them as this protein is synthesized by the liver, and its concentration decreases in advanced fibrosis. Steatosis was calculated by means of the fat fraction (FF [%]) between fat and water liver signals in “fat-only images” (the subtraction of in-phase [IP] images and out-of-phase [OOP] images) and in “water-only images” (the addition of IP and OOP images). Liver iron concentration (LIC [µmol/g]) was obtained by the transverse relaxation time (T2* [ms]) using Gandon’s method with multiple echo times (TE) in T2-weighted IP and OOP images. The preliminary results showed that there is an inverse relationship (r = −0.9662) between the T1 relaxation times (ms) and HSA concentrations (%). Steatosis was determined with FF > 6.4% and when the liver signal was greater than the paravertebral muscles signal, and thus, the liver appeared hyperintense in fat-only images. Hepatic iron overload was detected with LIC > 36 µmol/g, and in these cases, the liver signal was smaller than the paravertebral muscles signal, and thus, the liver behaved as hypointense in IP images.
Use of the Taguchi Method to Optimize an Immunodetection System for Quantitative Analysis of a Rapid Test
Authors: Lin Kai-Wen , Chang Yu-Chi
Published: June 2021

In this research, the Taguchi method was used to optimize the detection accuracy and reproducibility of an immunodetection system used for a quantitative analysis of a rapid test. Furthermore, the standard deviation (SD) and coefficient of variation (CV) between the theoretical value and the measured value of the self-made simulated rapid test became smaller, and the linearity became higher. The results thus indicated that the immunodetection system became more reliable. In the present research, a camera was used to capture an image containing the control line (C line) and the test line (T line) in the self-made simulated rapid test. The captured image was then analyzed, and the grayscales of the C line and T line were calculated. The Taguchi method was used to adjust the light intensity of the light-emitting diode (LED) and the camera parameters in the immunodetection system to determine the optimal parameters by which to optimize the performance of the immunodetection system. The goal of the present research was to obtain a measurement with a minimum SD and CV between the detected grayscales and the grayscales of the self-made simulated rapid test, thus indicating successful development of a practical, stable, and accurate immunodetection system. To mimic the color expression in an actual rapid test, the ratio of the red, green, blue (RGB) components of the self-made simulated rapid test had to be adjusted to closely fit the color expression of the actual rapid test. After the RGB ratio was set, the Taguchi method was used to optimize the parameters for the purpose of detection. When the optimal parameters were found, the signal-to-noise ratio (S/N ratio) had been increased from −12.89 dB to −10.91 dB, which means the accuracy of the color detection had been improved. Compared to the original detection system, the quality loss had been reduced to 33.1%.
sFlt-1, Not PlGF, Is Related to Twin Gestation Choronicity in the First and Third Trimesters of Pregnancy
Authors: Kozłowski Szymon , Stelmaszczyk-Emmel Anna , Szymusik Iwona , Saletra-Bielińska Aleksandra , Brawura-Biskupski-Samaha Robert , Pietruski Paweł , Osińska Agnieszka , Kosińska-Kaczyńska Katarzyna
Published: June 2021

Background: Preeclampsia occurs more often in dichorionic than in monochorionic twin pregnancy. We hypothesize that serum concentrations of biomarkers: placental growth factor (PlGF), serum soluble fms-like tyrosine kinase-1 (sFlt-1), and endoglin (Eng) differ between monochorionic and dichorionic twin pregnancies. Methods: A prospective observational study including 43 monochorionic and 36 dichorionic twin gestation was conducted. Blood samples were collected twice from all participants: between 11 + 0 and 13 + 6 and between 32 + 0 and 34 + 0 weeks of gestation. PlGF, sFlt-1 and Eng were measured using immnunoenzymatic assays. Results: We found a significantly higher concentration of sFlt-1 in dichorionic in comparison to monochorionic pregnancies in both the first and third trimesters. PlGF and sEng levels did not differ between mono- and dichorionic gestation in both study periods. sFlt-1 level was related to twin gestation chorionicity, while PlGF expression was not. PlGF, sFlt-1 and sEng concentrations increased significantly during gestation and were much higher in the third trimester compared to the values measured in the first trimester. Conclusions: Angiogenic biomarkers expression differ between dichorionic and monochorionic twin pregnancy. The sFlt-1 level is related to chorionicity of a twin gestation.
Automated Radiology Alert System for Pneumothorax Detection on Chest Radiographs Improves Efficiency and Diagnostic Performance
Authors: Kao Cheng-Yi , Lin Chiao-Yun , Chao Cheng-Chen , Huang Han-Sheng , Lee Hsing-Yu , Chang Chia-Ming , Sung Kang , Chen Ting-Rong , Chiang Po-Chang , Huang Li-Ting , Wang Bow , Liu Yi-Sheng , Chiang Jung-Hsien , Wang Chien-Kuo , Tsai Yi-Shan
Published: June 2021

We aimed to set up an Automated Radiology Alert System (ARAS) for the detection of pneumothorax in chest radiographs by a deep learning model, and to compare its efficiency and diagnostic performance with the existing Manual Radiology Alert System (MRAS) at the tertiary medical center. This study retrospectively collected 1235 chest radiographs with pneumothorax labeling from 2013 to 2019, and 337 chest radiographs with negative findings in 2019 were separated into training and validation datasets for the deep learning model of ARAS. The efficiency before and after using the model was compared in terms of alert time and report time. During parallel running of the two systems from September to October 2020, chest radiographs prospectively acquired in the emergency department with age more than 6 years served as the testing dataset for comparison of diagnostic performance. The efficiency was improved after using the model, with mean alert time improving from 8.45 min to 0.69 min and the mean report time from 2.81 days to 1.59 days. The comparison of the diagnostic performance of both systems using 3739 chest radiographs acquired during parallel running showed that the ARAS was better than the MRAS as assessed in terms of sensitivity (recall), area under receiver operating characteristic curve, and F1 score (0.837 vs. 0.256, 0.914 vs. 0.628, and 0.754 vs. 0.407, respectively), but worse in terms of positive predictive value (PPV) (precision) (0.686 vs. 1.000). This study had successfully designed a deep learning model for pneumothorax detection on chest radiographs and set up an ARAS with improved efficiency and overall diagnostic performance.
The Promises and Limitations of Educational Tiers for Special and Inclusive Education
Authors: Kauffman James M.
Published: June 2021

Making public school accommodating of all learners such that the need for special education is obviated, or at least reduced, has long been a desideratum of educators. Various strategies for making general public education more accommodating of students with disabilities have been tried. The most recent efforts to improve the general education of students with disabilities involve various models of tiered education. Educational tiers can be logical and advantageous in some ways, holding promise for improving general education, but they do not address the core problems of special education. Special education is still needed as part of inclusive education.