bluuebunny/crossref_metadata_2025 huggingface dataset:
Accounting for and Comparison of Greenhouse Gas (GHG) Emissions between Crop and Livestock Sectors in China
Authors: Han Jinyu , Qu Jiansheng , Wang Dai , Maraseni Tek Narayan
Published: September 2023

The synergistic greenhouse gas (GHG) emission reduction of the crop production (CP) and livestock farming (LF) sectors is of great significance for food security and low-carbon development, especially for China, the world leader in agricultural production. In this paper, the GHG emissions from the CP and LF sectors are accounted for and compared, and the spatial econometric model is adopted for comparative study based on the panel data from 1997 to 2021. The results show that: (1) The total amount and intensity of GHG emissions from both sectors showed obvious spatial heterogeneity and spatial dependence, and the spatial distribution pattern was relatively stable. (2) The influence of each factor on the GHG intensity and spatial characteristics of CP and LF varies widely. For the CP sector, economic development (local effect −0.29, adjacent effect +1.13), increased urbanization rate (−0.24, +0.16), agricultural structure (−0.29, +0.05), and urban-rural disparity (−0.03, +0.17) all reduce the GHG intensity of local region, while increasing the GHG intensity of its adjacent areas, signifying leakage. The economic structure (+0.06, +0.16), agricultural finance support (+0.02, +0.26), mechanization level (+0.05, +0.03), and land occupancy rate (+0.54, +0.44) all play a role in increasing the GHG intensity of CP in the local region and its adjacent areas. The disaster degree (−0.03, −0.03) also reduced the GHG intensity of CP. For the LF sector, economic structure (+0.08, +0.11), urban-rural disparity (+0.11, +0.21), agricultural development level (+0.03, +0.50), and increased land occupancy rate (+0.05, +0.01) can improve the GHG intensity of the one region and adjacent areas. Economic development (+0.03, −0.15), urbanization rate (+0.04, −0.30), agricultural structure (+0.09, −0.03), and disaster degree (+0.02, −0.06) can increase the GHG intensity of the local region while reducing the GHG intensity of adjacent areas. Based on the results, under the background of carbon peaking and carbon neutralization(dual-carbon) goals, this study first puts forward collaborative emission reduction measures for CP and LF, respectively, then further rises to sector synergy and regional synergy, and constructs the countermeasure system framework of collaborative emission reduction from three levels, to provide guidance and reference for the realization of dual goals of agricultural GHG reduction and food security.
Simulated Runoff and Erosion on Soils from Wheat Agroecosystems with Different Water Management Systems, Iran
Authors: Sharafi Saeed , Mohammadi Ghaleni Mehdi , Dragovich Deirdre
Published: September 2023

In developing countries, the demand for food has increased with significant increases in population. Greater demands are therefore being placed on the agricultural sector to increase production. This has led to increased soil erosion, especially in arid and semi-arid regions. The aim of this study was to simulate runoff and erosion on soils of three different wheat agroecosystems (rainfed farming, traditional irrigation, and industrial irrigation systems). The effect of variations in soil texture, slopes (1, 3 and 5%) and rainfall intensity (10, 25 and 40 mm h−1) on runoff volume, runoff coefficient, sediment concentrations, and sediment loss (soil erosion) were recorded for soils from each management system. Soil chemical properties (pH, EC) and organic matter were not significantly related to soil erosion. Analysis of variance showed significant differences in soil erosion and runoff coefficients when slopes were increased from 1 to 5 percent. The highest soil erosion was recorded on a slope of 5% with a rainfall intensity of 40 mm h−1, and the lowest on a slope of 1% with a rainfall intensity of 10 mm h−1. Of the three management systems, the highest runoff volume, runoff coefficient, sediment concentration and soil erosion occurred on soils from the traditional irrigation treatment, with a soil texture of sandy loam, slopes of 5% and rainfall intensity of 40 mm h−1. Results of the study indicated that the influence of slope and rainfall intensity on runoff volume, runoff coefficient, sediment concentration and soil erosion varies with soil texture and agroecosystem. These results can be usefully applied to agricultural land use planning and water management systems for reducing soil erosion at regional and on-farm levels.
Study on the Causes of Differences in Cropland Abandonment Levels among Farming Households Based on Hierarchical Linear Model—13,120 Farming Households in 26 Provinces of China as an Example
Authors: Wang Xiangdong , Zhao Decheng
Published: September 2023

Cropland abandonment is a widespread phenomenon with an increasing trend around the world, including China. Cropland abandonment is the result of a multi-scale and multifactor nested structure. In order to comprehensively identify the individual and background effects, this study explores the causes of the differences in farmland abandonment levels at two levels: farm household and village, based on a hierarchical linear model. The results show that (1) 83.63% of the differences in farm abandonment rates are differences in farm households, while 16.37% are differences in their villages; (2) several factors, including whether the head of household is healthier, per capita cropland area, ratio of transferred farmland, possession of large-scale agricultural production machinery or livestock for agricultural production, ratio of agricultural income, and whether there are village cadres, have a significant negative impact on abandonment rates at the farm household level, while at the village level, commuting distance, whether it is a suburb of a large or medium-sized city, topography of the village is plain or not, and the ratio of the number of people in agricultural production in the village have a significant positive impact on abandonment rates. Furthermore, whether it has experienced land expropriation has a significant positive effect on the abandonment rate; (3) commuting distance weakens the negative correlation between the ratio of transferred cropland and the ratio of agricultural income and the abandonment rate. In addition, whether it is a suburb of a large or medium-sized city strengthens the negative correlation between whether it owns large-scale agricultural production machinery or livestock and the abandonment rate, and whether the topography of the village is plain weakens the negative correlation between the possession of large agricultural production machinery or livestock for agricultural production and the agricultural income ratio and the abandonment rate. Furthermore, ratio of the number of people in agricultural production in the village weakens the negative correlation between the ratio of transferred cropland and abandonment rate, and whether the village has experienced land expropriation strengthens the negative correlation between the ratio of agricultural income and abandonment rate.
Eco-Environment Quality Response to Climate Change and Human Activities on the Loess Plateau, China
Authors: Zhang Xun , Gao Zhaoliang , Li Yonghong , Sun Guanfan , Cen Yunfeng , Lou Yongcai , Yao Yihang , Liu Wenbo
Published: September 2023

Climate change and human activities have caused a range of impacts on the ecological environment. The Loess Plateau (LP) is critical to the stability and health of ecosystems in central and western China, but there is still a lack of research on spatial and temporal heterogeneity in the effects of climate and human activities on the EEQ of the LP. We quantified the ecological environment quality of the study area from 2001 to 2019 based on the improved remote sensing ecological index (RSEI-2) and studied the spatial and temporal evolution of EEQ and its drivers during this period by trend analysis and multiscale geographic weighted regression (MGWR) model. The EEQ of the LP showed an increasingly slowing trend during 2001–2019, with apparent spatial heterogeneity, the south-central part was the hot spot area of change, and the center of gravity of change shifted 124.56 km to the southwest. The driving effects and ranges of each factor changed over time during the study period, and the positive effects of precipitation (PRE) and temperature (TEM) on the EEQ of the southern LP became more apparent, but the negative effects of TEM on the northwestern part have expanded. The negative effect of the intensity of land utilization (LUI) has increased from north to south and has the most profound impact, while population growth has less impact on the central region. The results of this research indicate that the execution of the Grain to Green Program (GGP) in the LP over the last two decades has been effective, but more attention should be paid to the maintenance of the restoration effect in the central region and the reasonable development of the land in the southern area. This research can enhance the comprehension of alterations in ecological factors that impact the environment of the LP. Additionally, it serves as a foundation for investigating strategies for ecological preservation and sustainable land development.
Performance of Deep-Learning Solutions on Lung Nodule Malignancy Classification: A Systematic Review
Authors: Liang Hailun , Hu Meili , Ma Yuxin , Yang Lei , Chen Jie , Lou Liwei , Chen Chen , Xiao Yuan
Published: September 2023

Objective: For several years, computer technology has been utilized to diagnose lung nodules. When compared to traditional machine learning methods for image processing, deep-learning methods can improve the accuracy of lung nodule diagnosis by avoiding the laborious pre-processing step of the picture (extraction of fake features, etc.). Our goal is to investigate how well deep-learning approaches classify lung nodule malignancy. Method: We evaluated the performance of deep-learning methods on lung nodule malignancy classification via a systematic literature search. We conducted searches for appropriate articles in the PubMed and ISI Web of Science databases and chose those that employed deep learning to classify or predict lung nodule malignancy for our investigation. The figures were plotted, and the data were extracted using SAS version 9.4 and Microsoft Excel 2010, respectively. Results: Sixteen studies that met the criteria were included in this study. The articles classified or predicted pulmonary nodule malignancy using classification and summarization, using convolutional neural network (CNN), autoencoder (AE), and deep belief network (DBN). The AUC of deep-learning models is typically greater than 90% in articles. It demonstrated that deep learning performed well in the diagnosis and forecasting of lung nodules. Conclusion: It is a thorough analysis of the most recent advancements in lung nodule deep-learning technologies. The advancement of image processing techniques, traditional machine learning techniques, deep-learning techniques, and other techniques have all been applied to the technology for pulmonary nodule diagnosis. Although the deep-learning model has demonstrated distinct advantages in the detection of pulmonary nodules, it also carries significant drawbacks that warrant additional research.
The Clinical Significance of Circulating Tumor DNA for Minimal Residual Disease Identification in Early-Stage Non-Small Cell Lung Cancer
Authors: Verlicchi Alberto , Canale Matteo , Chiadini Elisa , Cravero Paola , Urbini Milena , Andrikou Kalliopi , Pasini Luigi , Flospergher Michele , Burgio Marco Angelo , Crinò Lucio , Ulivi Paola , Delmonte Angelo
Published: September 2023

Lung cancer (LC) is the deadliest malignancy worldwide. In an operable stage I–III patient setting, the detection of minimal residual disease (MRD) after curative treatment could identify patients at higher risk of relapse. In this context, the study of circulating tumor DNA (ctDNA) is emerging as a useful tool to identify patients who could benefit from an adjuvant treatment, and patients who could avoid adverse events related to a more aggressive clinical management. On the other hand, ctDNA profiling presents technical, biological and standardization challenges before entering clinical practice as a decisional tool. In this paper, we review the latest advances regarding the role of ctDNA in identifying MRD and in predicting patients’ prognosis, with a particular focus on clinical trials investigating the potential of ctDNA, the technical challenges to address and the biological parameters that influence the MRD detection.
Synthesis and Characterization of 2-(((2,7-Dihydroxynaphthalen-1-yl)methylene)amino)-3′,6′-bis(ethylamino)-2′,7′-dimethylspiro[isoindoline-1,9′-xanthen]-3-one and Colorimetric Detection of Uranium in Water
Authors: Mohammed Rahisa , Ogadi Peace , Seth Dennis M. , Vibho Amrutaa , Gallant Sarah K. , Waterman Rory
Published: September 2023

2-(((2,7-Dihydroxynaphthalen-1-yl)methylene)amino)-3′,6′-bis(ethylamino)-2′,7′-dimethylspiro[isoindoline-1,9′-xanthen]-3-one was synthesized using Rhodamine 6G hydrazide (prepared using literature methods) and commercially available 2,7-dihydroxynaphthalene-1-carbaldehyde via imine condensation. Structural characterization was performed using FT-IR, 1H-NMR, 13C-NMR, X-ray, and HRMS. This Schiff base shows promise as a ligand for the colorimetric analysis of uranium in water.
Basic Design Parameters Influencing on Axial Stiffness of the Spiral Wound Gasket
Authors: Jaszak Przemysław , Grzejda Rafał , Kluczyński Janusz , Zmarzły Paweł
Published: September 2023

The article presents the influence of important design parameters of a spiral gasket on axial stiffness and leakage level. These parameters were the angle of inclination of the central part of the spiral section, the length of the vertical part of the spiral section, and the degree of densification of the material filling the metal coils. The scope of work was divided into two stages. In the first, experimental tests were conducted to determine the stiffness and tightness of a standard spiral gasket at two extreme levels of densification of the filler material, and the elastic–plastic properties of expanded graphite, which is the filler material of the metal spirals, were determined. In the second stage, multivariate numerical calculations were carried out to determine the axial stiffness of the gasket and to evaluate the distribution of contact pressure on the sealing surface. A novel aspect of the work is the proposal of a mathematical model to estimate the averaged value of the modulus of elasticity of the filler material as a function of the degree of densification and the execution of an experimental plan that significantly allowed the adoption of a limited number of analysed model variants used in the numerical calculations.
The Origin of Anomalous Density Behavior of Silica Glass
Authors: Cheng Shangcong
Published: September 2023

The anomalous density–temperature relationship of vitreous silica with low hydroxyl content is explained by the formation of medium-range ordering structure in the glass transition process. The ordered medium-range structure has the shape of a “nanoflake” and consists of two layers of SiO4 tetrahedra, bonded by O atoms located in the middle of the structure. The nanoflakes interact with their surrounding structures through both covalent chemical bonds and van der Waals bonds. In the formation of the van der Waals bonds, the orientation of SiO4 tetrahedra can change, which results in an increase in distance between the nanoflakes and their surrounding structures. Thus, there is a slight volume enlargement associated with the formation of nanoflakes. Since the nanoflakes’ formation starts at a temperature near 1480 °C, and the population of the nanoflakes grows continuously as temperature decreases until about 950 °C, the bulk volume of silica glass increases in the temperature range from about 1480 °C to 950 °C. Therefore, the density anomaly of silica glass can be explained as a byproduct of forming of medium-range ordering structure in the silica glass transition.
Verification of Reinforced Surface Loose Layer of Zinc–Aluminum–Magnesium Steel Plate
Authors: Chen Junxue , Chen Zheng , Yang Junjiao
Published: September 2023

The corrosion resistance of zinc–aluminum–magnesium steel plates (Zn–Al–Mg steel plates) is significantly higher than that of galvanized steel plates. However, the unsatisfactory bonding performance of Zn–Al–Mg steel plates significantly limits their widespread application. In this study, X-ray photoelectron spectroscopy is employed to detect changes in the surface oxygen content of Zn–Al–Mg steel plates after different temperature treatments to confirm the existence of surface loose layers. In particular, changes in the surface oxygen content of the Zn–Al–Mg steel plates after the oxide layer is removed are investigated under saturated H2O vapor and O2 environmental conditions, and the cause of the formation of loose surface layers is determined. The uneven distribution of elements on the surface of the Zn–Al–Mg steel plates is investigated with scanning electron microscopy and energy dispersive spectroscopy. Nuclear magnetic resonance is employed to determine the size of the network spatial structure formed by silane coupling agents under different hydrolysis conditions and to further investigate the bonding performance of hydrolysate-modified Zn–Al–Mg steel plates. Several typical automotive adhesives are utilized to compare and examine the changes in the tensile strength of the Zn–Al–Mg steel plate bonding before and after modification with the silane coupling agent and analyze the structural damage of the adhesive at the bonding interface. The results confirm that the silane coupling agent strengthens the loose layer on the surface of the Zn–Al–Mg steel plate.
Mechanical and Plasma Electrolytic Polishing of Dental Alloys
Authors: Witzke Katharina , Kensbock Renko , Willsch Caroline Ulrike , Fricke Katja , Bekeschus Sander , Metelmann Hans-Robert
Published: September 2023

(1) Background: In dentistry, a reduction in surface roughness is established mostly by conventional mechanical polishing to hinder biofilm adhesion. This is time- and labor-intensive. Plasma electrolytic polishing is believed to be an effective finishing method due to the reduced treatment time and materials used for applications in dentistry. (2) Methods: Co-Cr-Mo dental alloy samples were sandblasted and prepared with either plasma electrolytic or conventional mechanical polishing. Evaluation of the polishing methods was obtained by atomic force microscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy. (3) Results: The sandblasted samples showed the highest surface roughness (Heraenium® Sun 991 ± 288 nm; Wironit® 1187 ± 331 nm). Our results show that with plasma electrolytic polishing, Co-Cr-Mo surfaces can be polished with a surface roughness in the nanometer range, comparable to those achieved by conventional mechanical polishing. Conventional mechanical polishing (Heraenium® Sun 134 ± 23 nm; Wironit® 114 ± 11 nm) provided lower surface roughness values compared to plasma electrolytic polishing (Heraenium® Sun 288 ± 94 nm; Wironit® 261 ± 49 nm). We anticipate our pilot study as a starting point for future studies to refine process parameters and quantitative microbiological assays. (4) Conclusions: Plasma electrolytic polishing might have a promising future for polishing dental alloys.
Rotor Asymmetry Detection in Wound Rotor Induction Motor Using Kalman Filter Variants and Investigations on Their Robustness: An Experimental Implementation
Authors: John Basha Furzana , Somasundaram Kumar
Published: September 2023

This paper analyzes the performance of Kalman filter-based estimators for robust filtering and rotor asymmetry detection in wound rotor induction machines (WRIMs) using real-time data. Filter models were designed based on an extended model of WRIMs. The detection of rotor asymmetry was achieved by estimating the states of rotor resistance and speed using four filters. The sensitivity of the parameters under healthy and asymmetry conditions was thoroughly analyzed and categorized as low, medium, and high sensitivity parameters. Robust model-based estimators were designed to minimize the probability of false alarms. The performance analysis demonstrated that the dual unscented Kalman filter (DUKF) outperformed other Kalman filters such as the extended Kalman filter (EKF), dual extended Kalman filter (DEKF), and unscented Kalman filter (UKF) for state estimation of WRIM.
Inhomogeneous Canham–Helfrich Abscission in Catenoid Necks under Critical Membrane Mosaicity
Authors: Santiago José Antonio , Monroy Francisco
Published: September 2023

The mechanical effects of membrane compositional inhomogeneities are analyzed in a process analogous to neck formation in cellular membranes. We cast on the Canham–Helfrich model of fluid membranes with both the spontaneous curvature and the surface tension being non-homogeneous functions along the cell membrane. The inhomogeneous distribution of necking forces is determined by the equilibrium mechanical equations and the boundary conditions as considered in the axisymmetric setting compatible with the necking process. To establish the role played by mechanical inhomogeneity, we focus on the catenoid, a surface of zero mean curvature. Analytic solutions are shown to exist for the spontaneous curvature and the constrictive forces in terms of the border radii. Our theoretical analysis shows that the inhomogeneous distribution of spontaneous curvature in a mosaic-like neck constrictional forces potentially contributes to the membrane scission under minimized work in living cells.
Microstructure, Corrosion and Mechanical Properties of Magnesium Alloys
Authors: Zengin Hüseyin
Published: September 2023

The properties of magnesium (Mg) and its alloys such as uniquely high specific strength (strength-to-density ratio), good castability, excellent machinability, adequate high-temperature formability and high damping capacity have garnered significant interest from researchers and product designers, especially in the automotive and aerospace industries, for many years [...]
Stable Isotope Dilution Analysis (SIDA) to Determine Metabolites of Furan and 2-Methylfuran in Human Urine Samples: A Pilot Study
Authors: Kremer Jonathan Isaak , Karlstetter Dorothea , Kirsch Verena , Bohlen Daniel , Klier Carina , Rotermund Jan , Thomas Hannah , Lang Lukas , Becker Hanna , Bakuradze Tamara , Stegmüller Simone , Richling Elke
Published: September 2023

Furan and 2-methylfuran (2-MF) are food contaminants that are classified as potentially carcinogenic to humans. The main source of exposure for adults via food is coffee consumption. Furan and 2-MF are volatile, which complicates exposure assessment because their content measured in food prior to consumption does not afford a reliable dosimetry. Therefore, other ways of exposure assessment need to be developed, preferably by monitoring exposure biomarkers, e.g., selected metabolites excreted in urine. In this study, cis-2-buten-1,4-dial (BDA)-derived urinary furan metabolites Lys-BDA (l-2-amino-6-(2,5-dihydro-2-oxo-1H-pyrrol-1-yl)hexanoic acid), AcLys-BDA (l-2-(acetylamino)-6-(2,5-dihydro-2-oxo-1H-pyrrol-1-yl)hexanoic acid) and GSH-BDA (N-[4-carboxy-4-(3-mercapto-1H-pyrrol-1-yl)-1-oxobutyl]-l-cysteinyl-glycine cyclic sulfide), as well as acetyl acrolein (AcA, 2-oxo-pent-2-enal)-derived metabolites Lys-AcA (l-2-(acetylamino)-6-(2,5-dihydro-5-methyl-2-oxo-1H-pyrrol-1-yl)-hexanoic acid) and AcLys-AcA (l-2-amino-6-(2,5-dihydro-5-methyl-2-oxo-1H-pyrrol-1-yl)-hexanoic acid) and their stable isotopically labeled analogs, were synthesized and characterized through NMR and MS, and a stable isotope dilution analysis (SIDA) with UPLC-ESI-MS/MS was established. As a proof of concept, urinary samples of a four-day human intervention study were used. In the frame of this study, ten subjects ingested 500 mL of coffee containing 0.648 µmol furan and 1.059 µmol 2-MF. Among the furan metabolites, AcLys-BDA was the most abundant, followed by Lys-BDA and GSH-BDA. Exposure to 2-MF via the coffee brew led to the formation of Lys-AcA and AcLys-AcA. Within 24 h, 89.1% of the ingested amount of furan and 15.4% of the ingested amount of 2-MF were detected in the urine in the form of the investigated metabolites. Therefore, GSH-BDA, Lys-BDA, AcLys-BDA, Lys-AcA and AcLys-AcA may be suitable as short-term-exposure biomarkers of furan and 2-MF exposure.
Nutritional, Antioxidant, Antimicrobial, and Anticholinesterase Properties of Phyllanthus emblica: A Study Supported by Spectroscopic and Computational Investigations
Authors: Orabi Mohamed A. A. , Hasan Aso Hameed , AbouZid Sameh F. , El Amir Dalia , Hetta Mona H. , Awadh Ahmed Abdullah Al , Alqahtani Omaish Salman , Hatano Tsutomu , El-Shanawany Mohamed A.
Published: September 2023

Dietary fruits and vegetables play a vital role as food and drugs and are the main sources of antioxidant defences against degenerative diseases, such as brain dysfunctions, cardiovascular diseases, immune system deteriorations, and cancers, brought on by oxidative damage. Phyllanthus emblica is a significant herbal remedy used in conventional medicine to recover lost strength and power. In this research, the potential value of Phyllanthus emblica as a food and drug is researched. The total phenolic, total flavonoid, and total tannin contents as well as the nutritional value, vitamin C, vitamin E, and mineral contents of different organs of P. emblica were evaluated. The antioxidant and antimicrobial activities of extracts and fractions of different organs of P. emblica were determined. A total of eleven flavonoids, simple phenolic, tannin-related phenolic, and tannin molecules were isolated from a hydroalcoholic extract of the leaves and fruits. The structures were identified by spectroscopic data and comparison with the literature values as gallic acid (1), naringenin 7-O-(6″-O-galloyl)-β-D-glucopyranoside (2), 3,3′-di-O-methyl ellagic acid-4′-O-β-d-glucopyranoside (3), 1-O-galloyl glycerol (4), 1,6-di-O-galloyl-β-d-glucopyranoside (5), flavogallonic acid bislactone (6), corilagin (7), ethyl gallate (8), urolithin M5 (9), (E)-p-coumaroyl-1-O-β-d-glucopyranoside (10), and 1,2,4,6-tetra-O-galloyl-β-d-glucopyranoside (11). Among them, compounds 3 and 10 are first isolated from the plant. Molecular docking was performed to investigate the comparative interactions between positive controls (galantamine and donepezil) and selected compounds utilizing acetylcholinesterase (4EY7) as a target receptor. Results exhibited the potency of these compounds against the target receptor. In summary, P. emblica has a wealth of minerals, vitamins C and E, and polyphenolic phytochemicals that may work together to treat infectious disease, prevent and/or treat oxidative-damage-related illnesses including Alzheimer’s disease.
Genistein Suppresses IL-6 and MMP-13 to Attenuate Osteoarthritis in Obese Diabetic Mice
Authors: Lopez Janelle , Al-Nakkash Layla , Broderick Tom L. , Castro Monica , Tobin Brielle , Plochocki Jeffrey H.
Published: September 2023

Type 2 diabetes mellitus and osteoarthritis (OA) often present as comorbidities. We examined the role of plasma IL-6, chondrocyte MMP-13, and col10a expression in the development of OA in obese diabetic mice. We further investigated dietary genistein and exercise training as potential mitigators of OA. One hundred adult mice (50 females, 50 males) aged 6 weeks were randomized into 5 groups, including lean controls, obese diabetic controls, and obese diabetic mice treated with genistein, exercise training, and genistein plus exercise training. The obese diabetic state was induced by feeding the mice a high-fat, high-sugar diet. Genistein was incorporated into the diet at a concentration of 600 mg genistein/kg. Exercise training was performed on a treadmill and consisted of daily 30 min sessions at 12 m/min, 5 days/week for a 12-week period. After treatment, plasma was collected, and proximal tibias were removed for analysis. Plasma IL-6 and MMP-13 were elevated while col10a was reduced in obese diabetic mice in comparison to lean controls. Dietary genistein treatment reduced IL-6 and MMP-13 expression and increased col10a expression. Histological examination of articular cartilage showed reduced thickness of the uncalcified zones and proteoglycan content in the cartilage of diabetic mice in comparison to mice fed genistein. Exercise training had no significant effect. In conclusion, genistein (and not exercise training) attenuates OA by reducing IL-6 and MMP-13 expression in diabetic mice.
Special Issue on Metabolic Adaptations in Cardiac and Skeletal Muscle during Acute and Chronic Exercise
Authors: Kolwicz Stephen C.
Published: September 2023

Research in the field of exercise physiology has evolved dramatically over the last century [...]
The Triterpenoid High-Performance Liquid Chromatography Analytical Profiles of the Mycelia of Ganoderma lucidum (lingzhi)
Authors: Chen Deng-Hai , Wang Jian-Yuan , Chen Mon-Tarng , Liu Yen-Chun , Chen Kuang-Dee
Published: September 2023

(1) Background: Ganoderic acids (GAs) are specific triterpenes of Ganoderma lucidum. The HPLC fingerprint profile of GAs of the fruiting body is well known, but their mycelial fingerprinting remains unclear. (2) Methods: An ethanol extract of the mycelium of G. lucidum (YK-01) was further purified via preparative HPLC. The triterpenoid compositions for four strains of G. lucidum and one strain of G. formosanum (purple lingzhi) were analyzed using HPLC. (3) Results: Nineteen lanostane triterpenes, including five new triterpenes, GA-TP (1), ganodermic acid Jc (GmA-Jc) (2), GmA-Jd (3), GA-TQ1 (4), and ganoderal B1 (5), and fourteen known triterpenes 6–19 were isolated from the ethanol extract. Their structures were identified by mass and extensive NMR spectroscopy. A green chemical HPLC analytical method was developed using ethanol and acetic acid as a mobile phase, and all isolated compounds can be well separated. These triterpenes comprise a unique HPLC chromatograph of the G. lucidum mycelium. All four G. lucidum strains showed the same HPLC chromatographic pattern, whereas G. formosanum displayed a different pattern. Quantitation methods for ganoderic acid T (10) and S (12) were also validated. (4) Conclusions: The triterpenoid HPLC analytical method can be used to identify the G. lucidum species and to determine the contents of GA-T and GA-S.
Rare Earth Element Characteristics in Coal Ash from the Jungar Energy Gangue Power Plant, Inner Mongolia, China
Authors: Huang Shaoqing , Ning Shuzheng , Zhang Degao , Cai Yuan , Yan Xiaoyun , Liu Kang , Xu Xiaotao
Published: September 2023

The coal and coal-bearing measures in the Jungar Coalfield in Inner Mongolia are characterized by rare earth element (REE) enrichment. Combustion in coal-fired power plants can lead to further enrichment of REEs in coal ash, which serves as a new potential source for REE extraction and smelting. Further, investigating the content, modes of occurrence, and transformation behavior of REEs during coal combustion may help in better understanding REE differentiation during coal combustion and facilitate the development of economically feasible REE recovery technologies. Therefore, in this study, we analyzed coal ash from the Jungar Energy Gangue Power Plant in Inner Mongolia via inductively coupled plasma mass spectrometry, X-ray diffraction, and scanning electron microscopy combined with energy-dispersive spectroscopy. Our results showed that the REE content of the feed coal was 220 μg/g, slightly higher than the average for global coal. Additionally, fly ash had a higher REE content (898 μg/g) than bottom ash, and its rare earth oxide content was approximately 1152 μg/g, which meets the industrial requirements. Bottom and fly ashes contained similar minerals; however, their relative abundances were different. Specifically, mullite, quartz, calcite, and gypsum were slightly more abundant in fly ash than in bottom ash, whereas amorphous solids were slightly more abundant in bottom ash than in fly ash. Furthermore, fly ash, dominated by Si- and Al-rich minerals, was composed of irregular particles of different shapes and sizes. It also contained monazite and REE fluoro-oxides, which possibly originated from the feed coal and had mineral structures that remained unchanged during coal combustion. Thus, the REE fluoro-oxides possibly resulted from the conversion of bastnaesite in the feed coal during combustion and thereafter became attached to the edge of the Si–Al minerals in the fly ash.
Formation of Highly Conductive Interfaces in Crystalline Ionic Liquid-Gated Unipolar MoTe2/h-BN Field-Effect Transistor
Authors: Saidov Kamoladdin , Razzokov Jamoliddin , Parpiev Odilkhuja , Yüzbasi Nur Sena , Kovalska Natalia , Blugan Gurdial , Ruzimuradov Olim
Published: September 2023

2H MoTe2 (molybdenum ditelluride) has generated significant interest because of its superconducting, nonvolatile memory, and semiconducting of new materials, and it has a large range of electrical properties. The combination of transition metal dichalcogenides (TMDCs) and two dimensional (2D) materials like hexagonal boron nitride (h-BN) in lateral heterostructures offers a unique platform for designing and engineering novel electronic devices. We report the fabrication of highly conductive interfaces in crystalline ionic liquid-gated (ILG) field-effect transistors (FETs) consisting of a few layers of MoTe2/h-BN heterojunctions. In our initial exploration of tellurium-based semiconducting TMDs, we directed our attention to MoTe2 crystals with thicknesses exceeding 12 nm. Our primary focus centered on investigating the transport characteristics and quantitatively assessing the surface interface heterostructure. Our transconductance (gm) measurements indicate that the very efficient carrier modulation with an ILG FET is two times larger than standard back gating, and it demonstrates unipolarity of the device. The ILG FET exhibited highly unipolar p-type behavior with a high on/off ratio, and it significantly increased the mobility in MoTe2/h-BN heterochannels, achieving improvement as one of the highest recorded mobility increments. Specifically, we observed hole and electron mobility values ranging from 345 cm2 V−1 s−1 to 285 cm2 V−1 s−1 at 80 K. We predict that our ability to observe the intrinsic, heterointerface conduction in the channels was due to a drastic reduction of the Schottky barriers, and electrostatic gating is suggested as a method for controlling the phase transitions in the few layers of TMDC FETs. Moreover, the simultaneous structural phase transitions throughout the sample, achieved through electrostatic doping control, presents new opportunities for developing phase change devices using atomically thin membranes.
Color Rendering Index over 95 Achieved by Using Light Recycling Process Based on Hybrid Remote-Type Red Quantum-Dot Components Applied to Conventional LED Lighting Devices
Authors: Baek Eunki , Kim Boseong , Kim Sohee , Song Juyeon , Yoo Jaehyeong , Park Sung Min , Lee Jong-Min , Ko Jae-Hyeon
Published: September 2023

Red color conversion materials have often been used in conventional white LEDs (light-emitting diodes) to enhance the insufficient deep-red component and thus improve the color-rendering property. Quantum dots (QDs) are one of the candidates for this due to their flexibility in controlling the emission wavelength, which is attributed to the quantum confinement effect. Two types of remote QD components, i.e., QD films and QD caps, were prepared and applied to conventional white LED illumination to improve the color-rendering properties. Thanks to the red component near 630 nm caused by the QD components, the color rendering indices (CRIs) of both Ra and R9 could be increased to over 95. It was found that both the diffusing nature of the reflector and the light recycling process in the vertical cavity between the bottom reflector and the top optical films play important roles in improving the color conversion efficiency of remote QD components. The present study showed that the proper application of remote QDs combined with a suitable optical cavity can control the correlated color temperature of the illumination over a wide range, thus realizing different color appearances of white LED illumination. In addition, a high CRI of over 95 could be achieved due to the sufficient excitation from fewer QDs, due to the strong optical cavity effect.
CO-Sprout—A Pilot Double-Blinded Placebo-Controlled Randomised Trial of Broccoli Sprout Powder Supplementation for Pregnant Women with COVID-19 on the Duration of COVID-19-Associated Symptoms: Study Protocol
Authors: Fields Neville J. , Palmer Kirsten R. , Rolnik Daniel L. , Yo Jennifer , Nold Marcel F. , Giles Michelle L. , Krishnaswamy Sushena , Serpa Neto Ary , Hodges Ryan J. , Marshall Sarah A.
Published: September 2023

Since its discovery in late 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been estimated to be responsible for at least 769.3 million infections and over 6.95 million deaths. Despite significant global vaccination efforts, there are limited therapies that are considered safe and effective for use in the management of COVID-19 during pregnancy despite the common knowledge that pregnant patients have a much higher risk of adverse outcomes. A bioactive compound found in broccoli sprout—sulforaphane—is a potent inducer of phase-II detoxification enzymes promoting a series of potentially beneficial effects notably as an antioxidant, anti-inflammatory, and anti-viral. A pilot, double-blinded, placebo-controlled randomised trial is to be conducted in Melbourne, Australia, across both public and private hospital sectors. We will assess a commercially available broccoli sprout extract in pregnant women between 20+0 and 36+0 weeks gestation with SARS-CoV-2 infection to investigate (i) the duration of COVID-19 associated symptoms, (ii) maternal and neonatal outcomes, and (iii) biomarkers of infection and inflammation. We plan to enrol 60 outpatient women with COVID-19 irrespective of vaccination status diagnosed by PCR swab or RAT (rapid antigen test) within five days and randomised to 14 days of oral broccoli sprout extract (42 mg of sulforaphane daily) or identical microcrystalline cellulose placebo. The primary outcome of this pilot trial will be to assess the feasibility of conducting a larger trial investigating the duration (days) of COVID-19-associated symptoms using a broccoli sprout supplement for COVID-19-affected pregnancies. Pregnant patients remain an at-risk group for severe disease following infection with SARS-CoV-2 and currently unclear consequences for the offspring. Therefore, this study will assess feasibility of using a broccoli sprout supplement, whilst providing important safety data for the use of sulforaphane in pregnancy.
The Effects of Ontario Menu Labelling Regulations on Nutritional Quality of Chain Restaurant Menu Items—Cross-Sectional Examination
Authors: Yang Yahan , Ahmed Mavra , L’Abbé Mary R.
Published: September 2023

Restaurant foods are associated with excessive energy intake and poor nutritional quality. In 2017, the Healthy Menu Choices Act mandated food service establishments with ≥20 outlets in Ontario to display the energy content on menus. To examine the potential impact of menu labelling, nutrition information for 18,760 menu items were collected from 88 regulated and 53 unregulated restaurants. Descriptive statistics were calculated for serving size, energy, saturated fat, sodium and total sugars. Quantile regression was used to determine the differences between regulated and unregulated restaurants. The energy content of menu items from regulated restaurants (median (95% CI): 320 kcal (310, 320)) was significantly lower than those from unregulated restaurants (470 kcal (460, 486), p < 0.001). Saturated fat, sodium and total sugars were significantly lower in regulated restaurants (4 g (4, 4), 480 mg (470, 490) and 7 g (6, 7), respectively) than in unregulated restaurants (6 g (6, 6), 830 mg (797, 862) and 8 g (8, 9), respectively, p < 0.001). This study showed that menu items from regulated restaurants had smaller serving size, lower levels of energy and nutrients of public health concern compared to those from the unregulated restaurants, suggesting potential downstream beneficial effects of menu labelling in lowering caloric content and nutrients of public health concern in foods.
Identification and Pathogenicity of Fusarium Isolated from Soybean in Poland
Authors: Olszak-Przybyś Hanna , Korbecka-Glinka Grażyna , Patkowska Elżbieta
Published: September 2023

Fungi belonging to the Fusarium genus are commonly isolated from soybean plants and seeds but not all of them are pathogenic. The aim of this study was to compare the pathogenicity among different Fusarium isolates obtained from soybean plants with disease symptoms originating from an experimental field located in the southeast of Poland. Nineteen fungal isolates were selected for the pathogenicity assay, including eight isolates of F. oxysporum, six isolates of F. graminearum, four isolates of F. culmorum and one isolate of F. redolens. Species identification of these isolates was carried out using microscopic methods and sequencing of two genes: translation elongation factor 1-alpha (TEF1) and RNA polymerase second largest subunit (RPB2). To our knowledge, this is the first report of F. redolens being isolated from soybean in Europe. The pathogenicity test was set up by fungal inoculation of healthy soybean seeds of three cultivars: Abelina, Atlanta and Mavka. Symptoms were assessed seven days after inoculation. Disease area percentage of Fusarium inoculated seeds was significantly higher compared to uninoculated control. Nineteen isolates differed in their aggressiveness as the median disease area percentage ranged between 5.0 and 88.0% depending on isolate. The obtained isolates of four Fusarium species may be used in the future screening of soybean cultivars for resistance to these pathogens.
One Health Spread of 16S Ribosomal RNA Methyltransferase-Harboring Gram-Negative Bacterial Genomes: An Overview of the Americas
Authors: Sellera Fábio Parra , Fuentes-Castillo Danny , Furlan João Pedro Rueda
Published: September 2023

Aminoglycoside antimicrobials remain valuable therapeutic options, but their effectiveness has been threatened by the production of bacterial 16S ribosomal RNA methyltransferases (16S-RMTases). In this study, we evaluated the genomic epidemiology of 16S-RMTase genes among Gram-negative bacteria circulating in the American continent. A total of 4877 16S-RMTase sequences were identified mainly in Enterobacterales and nonfermenting Gram-negative bacilli isolated from humans, animals, foods, and the environment during 1931–2023. Most of the sequences identified were found in the United States, Brazil, Canada, and Mexico, and the prevalence of 16S-RMTase genes have increased in the last five years (2018–2022). The three species most frequently carrying 16S-RMTase genes were Acinetobacter baummannii, Klebsiella pneumoniae, and Escherichia coli. The armA gene was the most prevalent, but other 16S-RMTase genes (e.g., rmtB, rmtE, and rmtF) could be emerging backstage. More than 90% of 16S-RMTase sequences in the Americas were found in North American countries, and although the 16S-RMTase genes were less prevalent in Central and South American countries, these findings may be underestimations due to limited genomic data. Therefore, whole-genome sequence-based studies focusing on aminoglycoside resistance using a One Health approach in low- and middle-income countries should be encouraged.
New Antimicrobials for Gram-Positive Sustained Infections: A Comprehensive Guide for Clinicians
Authors: Carcione Davide , Intra Jari , Andriani Lilia , Campanile Floriana , Gona Floriana , Carletti Silvia , Mancini Nicasio , Brigante Gioconda , Cattaneo Dario , Baldelli Sara , Chisari Mattia , Piccirilli Alessandra , Di Bella Stefano , Principe Luigi
Published: September 2023

Antibiotic resistance is a public health problem with increasingly alarming data being reported. Gram-positive bacteria are among the protagonists of severe nosocomial and community infections. The objective of this review is to conduct an extensive examination of emerging treatments for Gram-positive infections including ceftobiprole, ceftaroline, dalbavancin, oritavancin, omadacycline, tedizolid, and delafloxacin. From a methodological standpoint, a comprehensive analysis on clinical trials, molecular structure, mechanism of action, microbiological targeting, clinical use, pharmacokinetic/pharmacodynamic features, and potential for therapeutic drug monitoring will be addressed. Each antibiotic paragraph is divided into specialized microbiological, clinical, and pharmacological sections, including detailed and appropriate tables. A better understanding of the latest promising advances in the field of therapeutic options could lead to the development of a better approach in managing antimicrobial therapy for multidrug-resistant Gram-positive pathogens, which increasingly needs to be better stratified and targeted.
Correction: Van der Veken et al. Gastrointestinal Fluid Volumes in Pediatrics: A Retrospective MRI Study. Pharmaceutics 2022, 14, 1935
Authors: Van der Veken Matthias , Aertsen Michael , Brouwers Joachim , Stillhart Cordula , Parrott Neil , Augustijns Patrick
Published: September 2023

In the original publication [...]
Evaluation of Hydrothermal Treatment of Winter Wheat Grain with Ozonated Water
Authors: Paulikienė Simona , Žvirdauskienė Renata
Published: September 2023

Products must be cleaned or otherwise treated to keep them clean when they are prepared for further production or when they are supplied fresh to the consumer. Cereals have significantly lower settling losses than succulent agricultural products, but the risks that can arise from their hydrothermal treatment before milling—where the cereals are moistened and left to rest for 14 h (temperature 30 °C)—are often underestimated. This operation creates a favourable environment for the development of micro-organisms, which, if not destroyed, can continue throughout the processing stages and be passed on to the consumer. This study investigated the qualitative characteristics of winter wheat hydrothermally treated with ozonated water at a concentration of 1.51 ± 0.1 mg L−1, such as the amount of mould in the grains and flour, as well as the grain protein, moisture, gluten, sedimentation, starch and weight per hectolitre. For the assessment of these parameters, the account was taken of the State standard, which provides the grain class and the type of grain. The reduction in mould fungi after the treatment of the winter wheat grain with ozonated water ranged between 440 and 950 CFU g−1. The results of the microbiological analysis showed that the ozone treatment improved the mycological safety of the flour samples made from the grain from an average of 390 ± 110 CFU g−1 to 29 ± 12 CFU g−1. On the other hand, the treatment of kernels with ozonated water did not significantly affect the basic composition of the winter wheat grains.