bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Experimental Models to Decipher the Effects of Acetaminophen and NSAIDs on Reproductive Development and Health
Authors: Boizet-Bonhoure Brigitte , Déjardin Stéphanie , Rossitto Moïra , Poulat Francis , Philibert Pascal
Published: March 2022

Nonsteroidal anti-inflammatory drugs (NSAIDs), such as aspirin (acetylsalicylic acid), diclofenac and ibuprofen (IBU), and analgesic drugs, such as acetaminophen (APAP, or paracetamol), are widely used to treat inflammation and pain. APAP and IBU are over-the-counter drugs and are among the most commonly taken drugs in the first trimester of pregnancy, even in combination. Furthermore, these drugs and their metabolites are released in the environment, and can be frequently detected in wastewater, surface water, and importantly in drinking water. Although their environmental concentrations are much lower than the therapeutics doses, this suggests an uncontrolled low-dose exposure of the general population, including pregnant women and young children, two particularly at risk populations. Epidemiological studies show that exposure to these molecules in the first and second trimester of gestation can favor genital malformations in new-born boys. To investigate the cellular, molecular and mechanistic effects of exposure to these molecules, ex vivo studies with human or rodent gonadal explants and in vivo experiments in rodents have been performed in the past years. This review recapitulates recent data obtained in rodent models after in utero or postnatal exposure to these drugs. The first part of this review discusses the mechanisms by which NSAIDs and analgesics may impair gonadal development and maturation, puberty development, sex hormone production, maturation and function of adult organs, and ultimately fertility in the exposed animals and their offspring. Like other endocrine disruptors, NSAIDs and APAP interfere with endocrine gland function and may have inter/transgenerational adverse effects. Particularly, they may target germ cells, resulting in reduced quality of male and female gametes, and decreased fertility of exposed individuals and their descendants. Then, this review discusses the effects of exposure to a single drug (APAP, aspirin, or IBU) or to combinations of drugs during early embryogenesis, and the consequences on postnatal gonadal development and adult reproductive health. Altogether, these data may increase medical and public awareness about these reproductive health concerns, particularly in women of childbearing age, pregnant women, and parents of young children.
Nutrient Release from Vermicompost under Anaerobic Conditions in Two Contrasting Soils of Bangladesh and Its Effect on Wetland Rice Crop
Authors: Hoque Tahsina Sharmin , Hasan Ahmed Khairul , Hasan Md. Arefin , Nahar Nurun , Dey Debasish Kumer , Mia Shamim , Solaiman Zakaria M. , Kader Md. Abdul
Published: March 2022

Although its mechanism of action, particularly under wetland condition, is not clearly understood, vermicompost, a good source of humus and plant nutrients, has been used as organic manure in many parts of the world in order to increase crop production. Here, an anaerobic incubation study and a field study were conducted to observe the nutrient release pattern from vermicompost and its influence on performance and nutrient uptake in wetland rice. Two contrasting soils, viz. highly weathered terrace soil and very young floodplain soil, were subjected to anaerobic incubation, while the field trial was conducted in the terrace soil with control (no amendments), mineral fertilizer, vermicompost (10 t ha−1) + mineral fertilizer, cow dung (10 t ha−1) + mineral fertilizer, vermicompost (20 t ha−1) + mineral fertilizer and vermicompost (local farmer’s practice) + mineral fertilizer treatments. Results showed that there were significant increases in nitrogen (N) and phosphorous (P) release in floodplain soil but not in terrace soil, suggesting that nutrient release from vermicompost is soil-dependent. The performance of Boro rice in terms of yield and yield attributes improved significantly in the case of the integrated application of vermicompost with mineral fertilizer. Specifically, combined application of mineral nutrients and vermicompost (10 t ha−1) significantly increased grain yield by 25% compared to the control treatment. We believe that this occurred due to an improvement in supply and subsequent uptake of nutrients, especially N and P. Altogether, our results suggest that vermicompost could increase crop performance under field conditions, and, although these effects may not be significant in short-term incubation studies, they may be even larger in floodplain soil.
Methyl Benzoate as a Promising, Environmentally Safe Insecticide: Current Status and Future Perspectives
Authors: Mostafiz Md. Munir , Hassan Errol , Lee Kyeong-Yeoll
Published: March 2022

The widespread use of synthetic chemical pesticides beginning in the late 1930s has contributed to the development of insecticide resistance of many important species of pest insects and plants. Recent trends in pesticide development have emphasized the use of more environmentally benign control methods that take into consideration environmental, food safety, and human health. Biopesticides (e.g., naturally occurring pesticidal compounds) are alternative pest management tools that normally have no negative impact on human health or the environment. Here we review methyl benzoate, a relatively new botanical insecticide that occurs naturally as a metabolite in plants, and whose odor is an attractant to some insects. Since 2016, many studies have shown that methyl benzoate is an effective pesticide against a range of different agricultural, stored product, and urban insect pests. Methyl benzoate has several important modes of action, including as a contact toxicant, a fumigant, an ovicidal toxin, an oviposition deterrent, a repellent, and an attractant. In this review, we summarize various modes of action of methyl benzoate and its toxicity or control potential against various kinds of arthropods, including agricultural pests and their natural enemies, and pollinators. We conclude that methyl benzoate is a very promising candidate for use in integrated pest management under either greenhouse or field conditions.
Salinity Tolerance Characteristics of Marginally Located Rice Varieties in the Northernmost Rice-Growing Area in Europe
Authors: Székely Árpád , Szalóki Tímea , Pauk János , Lantos Csaba , Ibadzade Marks , Jancsó Mihály
Published: March 2022

Detailed experiments were performed to investigate the effects of the marginal cultivation of temperate japonica rice on salinity tolerance in different developmental stages and to detect new genetic resources for further research and breeding projects. Eight rice varieties were screened for salinity tolerance in a greenhouse at two developmental stages. During seedling-stage screening, stress was induced with a 12 dS m−1 NaCl solution. For reproductive-stage analysis, we applied continuous salinity stress (8.39 dS m−1 of soil extract at 25 °C (EC25)). We compared the marginal rice genotypes (japonica_1) to widely grown controls (those being Mediterranean japonica genotypes, referred as the japonica_2 group, which also included one indica genotype). Without stress, marginally cultivated genotypes had significantly higher potassium storage capacity (406 µmol g−1) in flag leaves than did japonica_2 (347) or indica (267). Moreover, the seeds contained twice as much sodium (5.27) as did the others (2.19 and 2.00). Based on root-to-grain allocation, we identified two sodium excluders (‘Dular’ and ‘Dáma’) and six includers under constant salinity stress. Among the includers, we found that ‘M488’ had extremely high sodium concentration in both the flag leaves (250.54 µmol g−1) and the grains (13.57 µmol g−1). Rice varieties bred for the edge of their habitat show special physiological patterns. The differences are mainly manifested as lower levels of antioxidant pigments, higher amounts of potassium in flag leaves, and higher sodium levels in grains under normal conditions. With the onset of stress, higher tillering ability, enhanced antioxidant pigment synthesis, and rapid potassium translocation from the root are the key mechanisms which help the plants to avoid serious damage caused by salinity.
Crop Residue Management Strategies to Reduce Nitrogen Losses during the Winter Leaching Period after Autumn Spinach Harvest
Authors: Frerichs Christian , Glied-Olsen Stephan , De Neve Stefaan , Broll Gabriele , Daum Diemo
Published: March 2022

In open-field vegetable production, high quantities of soil mineral nitrogen (Nmin) and N-rich crop residues often remain in the field at harvest. After the harvest of crops in autumn, this N can lead to considerable nitrate (NO3−) losses during the subsequent winter leaching period. In four field trials, different tillage depths (3–4, 10, 30 cm) and dates (early autumn, late autumn, early spring) were investigated to reduce N losses after growing spinach in the autumn. In a further treatment, the nitrification inhibitor 3,4-Dimethylpyrazole phosphate (DMPP) was directly applied to the crop residues. Potential N losses were calculated by a balance sheet approach based on Nmin concentration (0–90 cm), measured N mineralization and N uptake by catch crops. By postponing the tillage date from early to late autumn or spring, resprouting spinach stubbles acted as a catch crop, reducing N losses by up to 61 kg ha−1. However, if the spinach biomass collapsed, the N losses increased by up to 33 kg ha−1 even without tillage. The application of DMPP as well as the tillage depth were less effective. Overall, postponing tillage to spring seems to be the most promising approach for reducing N losses during the off-season.
Effects of Dietary Supplementation with Honeybee Pollen and Its Supercritical Fluid Extract on Immune Response and Fillet’s Quality of Farmed Gilthead Seabream (Sparus aurata)
Authors: Arena Rosaria , de Medeiros Adja Cristina Lira , Secci Giulia , Mancini Simone , Manuguerra Simona , Bovera Fulvia , Santulli Andrea , Parisi Giuliana , Messina Concetta Maria , Piccolo Giovanni
Published: March 2022

The awareness of the correlation between administered diet, fish health and products’ quality has led to the increase in the research for innovative and functional feed ingredients. Herein, a plant-derived product rich in bioactive compounds, such as honeybee pollen (HBP), was included as raw (HBP) and as Supercritical Fluid Extracted (SFE) pollen (HBP_SFE) in the diet for gilthead seabream (Sparus aurata). The experiment was carried out on 90 fish with an average body weight of 294.7 ± 12.8 g, divided into five groups, according to the administration of five diets for 30 days: control diet (CTR); two diets containing HBP at 5% (P5) and at 10% (P10) level of inclusion; two diets containing HBP_SFE, at 0.5% (E0.5) and at 1% (E1) level of inclusion. Their effects were evaluated on 60 specimens (336.2 ± 11.4 g average final body weight) considering the fish growth, the expression of some hepatic genes involved in the inflammatory response (il-1β, il-6 and il-8) through quantitative real-time PCR, and physico-chemical characterization (namely color, texture, water holding capacity, fatty acid profile and lipid peroxidation) of the fish fillets monitored at the beginning (day 0) and after 110 days of storage at −20 °C. The results obtained showed that the treatment with diet E1 determined the up-regulation of il-1β, il-6, and il-8 (p < 0.05); however, this supplementation did not significantly contribute to limiting the oxidative stress. Nevertheless, no detrimental effect on color and the other physical characteristics was observed. These results suggest that a low level of HBP_SFE could be potentially utilized in aquaculture as an immunostimulant more than an antioxidant, but further investigation is necessary.
Melatonin Application Alleviates Stress-Induced Photosynthetic Inhibition and Oxidative Damage by Regulating Antioxidant Defense System of Maize: A Meta-Analysis
Authors: Muhammad Ihsan , Yang Li , Ahmad Shakeel , Mosaad Ibrahim S. M. , Al-Ghamdi Abdullah Ahmed , Abbasi Arshad Mehmood , Zhou Xun-Bo
Published: March 2022

Melatonin is effective in modulating metabolism and regulating growth and development in many plants under biotic and abiotic stress. However, there is no systematic quantification of melatonin effects on maize growth, gas exchange, chlorophyll content, and the antioxidant defense system. A meta-analysis was conducted on thirty-two currently available published articles to evaluate the effect of stress types, study types, and maize varieties on response ratio (lnRR++) of “melatonin” to “control (no melatonin)” on plant growth, enzyme activities, gas exchange parameters, and photosynthetic pigments. Our findings revealed that melatonin application overall increased plant height, leaf area, root length, fresh and dry root weight and shoot weight, superoxide dismutase (SOD), peroxide (POD), catalase (CAT), ascorbate peroxidase (APX), soluble sugar and protein, photosynthetic rate, stomatal conductance, transpiration rate, chlorophyll, and carotenoid in maize leaf under stress conditions. In contrast, melatonin application decreased the levels of hydrogen peroxide (H2O2), superoxide anion (O2−), malondialdehyde (MDA), and electrolyte leakage. The categorical meta-analysis demonstrated that melatonin application to chilling stress resulted in higher SOD activity followed by salt stress. Melatonin application to all stress types resulted in higher POD, CAT and APX activities, except Cd stress, which had no effect on POD and decreased CAT by 38% compared to control. Compared to control, melatonin resulted in lower reactive oxygen species (ROS) and electrolyte leakage under no stress, Cd, drought, salt, lead, heat, and chilling stress in all study types (pot, growth chamber, hydroponic, and field), except O2 content which was not affected in pot and growth chamber studies. It was concluded that melatonin alleviates oxidative damage by improving stress tolerance, regulating the antioxidant defense system, and increasing leaf chlorophyll content compared to control.
Pulmonary Protein Oxidation and Oxidative Stress Modulation by Lemna minor L. in Progressive Bleomycin-Induced Idiopathic Pulmonary Fibrosis
Authors: Karamalakova Yanka , Stefanov Ivaylo , Georgieva Ekaterina , Nikolova Galina
Published: March 2022

Bleomycin (BLM) administration is associated with multifunctional proteins inflammations and induction of idiopathic pulmonary fibrosis (IPF). Lemna minor L. extract, a free-floating monocot macrophyte possesses antioxidant and anti-inflammatory potential. The aim of the study was to examine the protective effect of L. minor extract on lung protein oxidation and oxidative stress modulation by BLM-induced pulmonary fibrosis in Balb/c mice. For this purpose, the protein carbonyl content, advanced glycation end product, nitroxide protein oxidation (5-MSL), and lipid peroxidation (as MDA and ROS), in lung cells were examined. The histological examinations, collagen deposition, and quantitative measurements of IL-1β, IL-6, and TNF in lung tissues and blood were investigated. Intraperitoneal, BLM administration (0.069 U/mL; 0.29 U/kg b.w.) for 33 days, caused IPF induction in Balb/c mice. Pulmonary combining therapy was administered with L. minor at dose 120 mg/mL (0.187 mg/kg b.w.). L. minor histologically ameliorated BLM induced IPF in lung tissues. L. minor significantly modulated (p < 0.05) BLM-alterations induced in lung hydroxyproline, carbonylated proteins, 5-MSL-protein oxidation. Oxidative stress decreased levels in antioxidant enzymatic and non-enzymatic systems in the lung were significantly regulated (p < 0.05) by L. minor. L. minor decreased the IL-1β, IL-6, and TNF-α expression in lung tissues and plasma. The L. minor improves the preventive effect/defense response in specific pulmonary protein oxidation, lipid peroxidation, ROS identifications, and cytokine modulation by BLM-induced chronic inflammations, and could be a good antioxidant, anti-inflammatory, and anti-fibrotic alternative or IPF prevention involved in their pathogenesis.
Setting Up and Operating Electric City Buses in Harsh Winter Conditions
Authors: Vehviläinen Maarit , Lavikka Rita , Rantala Seppo , Paakkinen Marko , Laurila Janne , Vainio Terttu
Published: March 2022

The city of Tampere in Finland aims to be carbon-neutral in 2030 and wanted to find out how the electrification of public transport would help achieve the climate goal. Research has covered topics related to electric buses, ranging from battery technologies to lifecycle assessment and cost analysis. However, less is known about electric city buses’ performance in cold climatic zones. This study collected and analysed weather and electric city bus data to understand the effects of temperature and weather conditions on the electric buses’ efficiency. Data were collected from four battery-electric buses and one hybrid bus as a reference. The buses were fast-charged at the market and slow-charged at the depot. The test route ran downtown. The study finds that the average energy consumption of the buses during winter was 40–45% higher than in summer (kWh/km). The effect of cabin cooling is minor compared to the cabin heating energy needs. The study also finds that infrastructure needs to have enough safety margins in case of faults and additional energy consumption in harsh weather conditions. In addition, appropriate training for operators, maintenance and other personnel is needed to avoid disturbances caused by charging and excessive energy consumption by driving style.
A Combined Electromagnetic and Mechanical Approach for EU-DEMO Toroidal Field Coils
Authors: Giannini Lorenzo , Boso Daniela P. , Corato Valentina
Published: March 2022

The roadmap to fusion electricity of the European scientific program aims at the realization of the future DEMOnstration (DEMO) fusion power plant. In 2020, the pre-concept design phase of DEMO was completed, defining the concept and characteristics of the main magnets and structures of the machine. Sixteen toroidal D-shaped magnets, six poloidal annular coils and a central solenoid constitute the functioning system core. The reactor is subjected to huge mechanical loads, mainly due to the Lorentz force produced by the combination of the high magnetic fields and operative currents. As a consequence, the loading conditions are extremely demanding for the structural components, and it is crucial to complete a comprehensive static and fatigue assessment before proceeding with the next design iteration. This work focuses on the electromagnetic and structural analyses performed on the toroidal field coil system and its support structures to present the methodological approach developed. Exploiting the finite element method, a three-dimensional model has been defined to obtain the electromagnetic loads on the main time points of the reference plasma scenario and then transfer them to a related 3D structural model, corresponding to the discretization of the electromagnetic one. The structural model was used to obtain the displacement and stress fields at the various time points to perform the mechanical evaluation as well as the fatigue assessment.
A Novel Multiport DC-DC Converter for Enhancing the Design and Performance of Battery–Supercapacitor Hybrid Energy Storage Systems for Unmanned Aerial Vehicles
Authors: Fares Ahmed M. , Klumpner Christian , Sumner Mark
Published: March 2022

This paper proposes an integrated multiport non-isolated DC–DC converter system for integrating battery–supercapacitor hybrid energy storage with photovoltaics for solar-powered unmanned aerial vehicles applications. Compared to the traditional topologies used, the proposed converter allows a size reduction of at least 20% of the supercapacitor by maximizing the utilization of the rated energy capacity. In addition, by proposing to use a phase-shifted carrier modulation technique, the inductors’ current ripple is reduced, which enables a further reduction in the inductor size. These improvements in capability and performance of the proposed topology are experimentally validated on a 500 W PV/battery–supercapacitor integrated power system prototype.
Technology Behavior Model—Beyond Your Sight with Extended Reality in Surgery
Authors: Gong Xiaoxue , JosephNg Poh Soon
Published: March 2022

Extended Reality Smart Glasses is a new pattern that uses extended reality technology to present a visual environment that combines the physical and virtual worlds. However, the surgical technique using Smart Glasses implementation is still unknown, to the infancy in clinical surgery, derived to the limits of existing technology. This study researched the acceptability and possibility of XRSG for medical experts. It combines human seen behavioral control with information technology research to construct a new “Extended Reality Technology Behavior Model” using method Technology Acceptance Model and Theory of Planned Behavior. To improve the accuracy of the study, statistical analysis, exploratory analysis, and cross-sectional research triangulation were used to collect data in five hospitals in Malaysia using a convenience sampling method and a questionnaire on behavioral influences. From the collected data, PLS-SEM analysis was used to reflect the relationship between variables. The strong positive results suggest that using XRSG by medical experts helps to improve the composition, interactivity, standardization, and clarity of medical images, resulting in increased efficiency and reduced procedure time and felt the usefulness and ease of use of XRSG through their behavior, providing a basis for technology acceptance in surgery.
Numerical Simulation on Particulate Matter Emissions from a Layer House during Summer in Northeast China
Authors: Zhang Shuo , Zhou Lina , Jia Lexin , Li Jinsheng , Liu Biying , Yuan Yueming
Published: March 2022

Particulate matter (PM) emitted from livestock and poultry production contributes to atmospheric aerosol loading, affecting animal health and the surrounding atmospheric environment. However, developing and optimizing remediation technologies require a better understanding of air pollutant concentrations, the emission plumes, and the mechanism of emission. Previous studies have primarily focused on indoor air pollution research, while outdoor research is relatively rare. Field test research is not only costly but also consumes extensive amounts of time. The application of computational fluid dynamics (CFD) technology can save a lot of measurement time and repetitive labor, in order to better understand the diffusion fundamentals and spatial and temporal distribution differences of PM. This study monitored the PM concentrations of different particle sizes inside and outside a layer house with negative pressure ventilation in Northeast China during the summer of 2021. These data were also used to validate the three-dimensional simulation of the PM concentrations inside and outside of the layer house in various scenarios of wind directions at different times by CFD technology. Through correlation analysis, it is found that temperature was positively correlated with PM1 and PM2.5, and relative humidity and wind speed were negatively correlated with PM, which has a greater impact on PM10 and total suspended particulate (TSP). The particle size was proportional to the diffusion distance and diffusion height, time, and wind direction both have an impact on the spread of PM. Considering the environmental conditions in Northeast China, increasing the height of the fan by 1 m was suggested to reduce the diffusion of PM concentration. In addition, the diffusion patterns and transport paths in this study provide valuable information for improving control measures to minimize the influence of PM on both animal health and air quality.
Roles of Cullin-RING Ubiquitin Ligases in Cardiovascular Diseases
Authors: Diaz Stephanie , Wang Kankan , Sjögren Benita , Liu Xing
Published: March 2022

Maintenance of protein homeostasis is crucial for virtually every aspect of eukaryotic biology. The ubiquitin-proteasome system (UPS) represents a highly regulated quality control machinery that protects cells from a variety of stress conditions as well as toxic proteins. A large body of evidence has shown that UPS dysfunction contributes to the pathogenesis of cardiovascular diseases. This review highlights the latest findings regarding the physiological and pathological roles of cullin-RING ubiquitin ligases (CRLs), an essential player in the UPS, in the cardiovascular system. To inspire potential therapeutic invention, factors regulating CRL activities are also discussed.
Changes in Hippocampus and Amygdala Volume with Hypoxic Stress Related to Cardiorespiratory Fitness under a High-Altitude Environment
Authors: Wang Zhi-Xin , Su Rui , Li Hao , Dang Peng , Zeng Tong-Ao , Chen Dong-Mei , Wu Jian-Guo , Zhang De-Long , Ma Hai-Lin
Published: March 2022

The morphology of the hippocampus and amygdala can be significantly affected by a long-term hypoxia-induced inflammatory response. Cardiorespiratory fitness (CRF) has a significant effect on the neuroplasticity of the hippocampus and amygdala by countering inflammation. However, the role of CRF is still largely unclear at high altitudes. Here, we investigated brain limbic volumes in participants who had experienced long-term hypoxia exposure in Tibet (3680 m), utilizing high-resolution structural images to allow the segmentation of the hippocampus and amygdala into their constituent substructures. We recruited a total of 48 participants (48 males; aged = 20.92 ± 1.03 years) to undergo a structural 3T MRI, and the levels of maximal oxygen uptake (VO2max) were measured using a cardiorespiratory function test. Inflammatory biomarkers were also collected. The participants were divided into two groups according to the levels of median VO2max, and the analysis showed that the morphological indexes of subfields of the hippocampus and amygdala of the lower CRF group were decreased when compared with the higher CRF group. Furthermore, the multiple linear regression analysis showed that there was a higher association with inflammatory factors in the lower CRF group than that in the higher CRF group. This study suggested a significant association of CRF with hippocampus and amygdala volume, which may be related to hypoxic stress in high-altitude environments. A better CRF reduced physiological stress and a decrease in the inflammatory response was observed, which may be related to the increased oxygen transport capacity of the body.
Effects of Postnatal Caffeine Exposure on Absence Epilepsy and Comorbid Depression: Results of a Study in WAG/Rij Rats
Authors: Ilbay Gul , Dogan Zeynep Ikbal , Balıkcı Aymen , Erdogan Seyda , Karaoglan Kahilogulları Akfer
Published: March 2022

The present study aims to investigate effect of early caffeine exposure on epileptogenesis and occurrence of absence seizures and comorbid depression in adulthood. For this purpose, Wistar Albino Glaxo from Rijswijk (WAG/Rij) rats were enrolled in a control and two experimental groups on the 7th day after the delivery. The rats in experimental groups received either 10 or 20 mg/kg caffeine subcutaneously while animals in control group had subcutaneous injections of 0.9% saline. The injections started at postnatal day 7 (PND7) and were continued each day for 5 days. At 6–7 months of age, electroencephalogram (EEG) recordings and behavioral recordings in the forced swimming test, sucrose consumption/preference test and locomotor activity test were carried out. At 6 months of age, 10 mg/kg and 20 mg/kg caffeine-treated WAG/Rij rats showed increased immobility latency and active swimming duration in forced swimming test when compared with the untreated controls. In addition, 20 mg/kg caffeine treatment decreased immobility time. In sucrose preference/consumption tests, WAG/Rij rats in 10 mg/kg caffeine group demonstrated higher sucrose consumption and preference compared to untreated controls. The rats treated with 20 mg/kg caffeine showed higher sucrose preference compared to control rats. The exploratory activity of rats in the 10 mg/kg caffeine-treated group was found to be higher than in the 20 mg/kg caffeine-treated and control groups in the locomotor activity test. At 7 months of age, caffeine-treated animals showed a decreased spike-wave discharge (SWD) number compared to the control animals. These results indicate that postnatal caffeine treatment may decrease the number of seizure and depression-like behaviors in WAG/Rij rats in later life. Caffeine blockade of adenosine receptors during the early developmental period may have beneficial effects in reducing seizure frequency and depression-like behaviors in WAG/Rij rat model.
Physical Activity and Depression in Adolescents: Evidence from China Family Panel Studies
Authors: Gu Jiafeng
Published: March 2022

Depression in adolescents is a major public health disorder. The relationship between physical activity and risk of depression in adolescents was examined using three waves of data from the China Family Panel Studies in 2020. The risk of depression was significantly higher among adolescents who reported lower frequency and shorter duration of physical exercise than those who reported physical exercise more frequently and for a longer duration. The risk of depression was significantly higher among adolescents who reported intense physical exercise than those who reported little or no intense physical exercise. The amount of time spent on housework by adolescents is inversely associated with depression. These results provide somewhat stronger evidence for an activity–depression link than previous studies and suggest a differential role for different types of physical activity, such as exercise and housework. The overall model predicting depression in adolescents (LR chi-squared = 95.974, p < 0.001, Nagelkerke R-square = 0.183) was statistically significant. To effectively control depression in adolescents, the government, schools and parents need to act together to guide adolescents towards participation in appropriate physical activities. The appropriate level of physical activity is for adolescents to experience breathing, rapid heartbeat, and slight perspiration.
Optimization of the Use Time of a Shake Table with Specimen Preparation outside the Table Surface
Authors: Yadav Santosh , Sieffert Yannick , Vieux-Champagne Florent , Debove Laurent , Decret Damien , Malecot Yann , Garnier Philippe
Published: March 2022

The shake table test is one of the preferred techniques used to understand the dynamic response of a structure. However, due to the limited number of facilities available to perform such tests and their expensiveness, researchers often must rely on numerical models validated with the results of the static tests only. Moreover, most research papers concerning shake table tests lack details on how the tests were planned and executed. This paper explains the steps used for the preparation and execution of shake table tests on three reduced-scale buildings. These buildings were constructed outside the shake table surface, on a metallic base frame, and later moved to the shake table used for the tests in order to optimize the time of the experimental campaign. This approach enabled us to complete the tests in only 6 days. The approach presented in this paper may be helpful to researchers who want to increase the effectiveness of the available shake table facility and overcome the limitations of time and budget. Moreover, the solution presented in this article helps in the displacement of specimens without the use of a crane or other sophisticated hydraulic machinery. Thus, it could also be useful for testing specimens that have been aged and that are sensitive to displacements.
Dynamic Behavior of the Composite Steel–Concrete Beam Floor Systems under Free and Forced Vibration
Authors: Tahmasebinia Faham , Yip Cho Sum , Lok Chio Fai , Sun Yufan , Wu Junyi , Sepasgozar Samad M. E. , Marroquin Fernando Alonso
Published: March 2022

This paper aims to investigate the dynamic behavior of composite steel–concrete floor systems under both free and forced vibrations. A combination of numerical and analytical methods was comprehensively employed to calibrate the suggested solutions to extend the application of accurate numerical methods in future design purposes. Different commercial Finite Element Packages including ABAQUS/CAE and Strand7 were precisely utilized. The obtained results from the Finite Element Simulations were broadly compared with the available international design guidelines including British Standard BS 6472 and international standard ISO 10137. The first 10 active vibration modes in different composite steel–concrete beam floor systems were numerically investigated. Different concrete slabs by respecting the designated various types of secondary and primary steel beam components were comprehensively examined. It was found that the lengths of primary and secondary beams can considerably influence the computed fundamental frequencies and the response factors of the simulated composite floor system. Based on carrying out an extensive parametrical study, further practical recommendations were suggested to provide a reliable benchmark for structural designers.
Barriers to BIM-Based Life Cycle Sustainability Assessment for Buildings: An Interpretive Structural Modelling Approach
Authors: Onososen Adetayo , Musonda Innocent
Published: March 2022

With the emergence of Building Information Modelling (BIM) as central to construction design, planning, execution and maintenance, integration into the entire infrastructure sustainability process is imperative for achieving sustainable development. Despite its immense benefit of aiding compliance to sustainable construction, potential barriers continue to widen the gap in implementation. Therefore, this study adopts the “interpretive structural modelling approach” to advance a ranked structure of the interrelatedness of the barriers to integrating BIM in buildings sustainability assessment. The “Matrice d’Impacts croises-multipication applique a classement analysis (MICMAC)” was utilised to categorise the identified adoption barriers in the model. The identified barriers and relationship with themselves are valuable in discussing the challenges to BIM-based LCA and developing policies and design decisions to drive the process further. Further, it adds to the emerging discussion of BIM from the life cycle sustainability assessment perspective for infrastructure. The findings are critical for policy, stakeholders and extending the body of knowledge.
Inflammation and Prostate Cancer: A Multidisciplinary Approach to Identifying Opportunities for Treatment and Prevention
Authors: Huang Lanshan , LaBonte Melissa J. , Craig Stephanie G. , Finn Stephen P. , Allott Emma H.
Published: March 2022

Prostate cancer is a major cause of disease for men globally. Inflammation, an established hallmark of cancer, is frequently observed in the prostate, though its contribution to prostate cancer risks and outcomes is not fully understood. Prostate cancer is biologically and clinically heterogeneous, and there is now evidence that inflammation and immunological characteristics vary by the genomic and mutational landscape of the tumor. Moreover, it is now recognized that risk factor profiles vary between tumor subgroups, as defined by histopathological and molecular features. Here, we provide a review centered around the relationship between inflammation and prostate cancer, with a consideration of molecular tumor features and a particular focus on the advanced and lethal stages of disease. We summarize findings from epidemiological studies of the etiology and role of inflammation in prostate cancer. We discuss the pathology of prostate inflammation, and consider approaches for assessing the tumor immune microenvironment in epidemiological studies. We review emerging clinical therapies targeting immune biology within the context of prostate cancer. Finally, we consider potentially modifiable risk factors and corresponding lifestyle interventions that may affect prostate inflammation, impacting outcomes. These emerging insights will provide some hints for the development of treatment and prevention strategies for advanced and lethal prostate cancer.
Efficient Asymmetric Synthesis of (S)-N-Boc-3-hydroxypiperidine by Coexpressing Ketoreductase and Glucose Dehydrogenase
Authors: Gao Xinxing , Pei Qianqian , Zhu Nianqing , Mou Yi , Liang Jilei , Zhang Xin , Feng Shoushuai
Published: March 2022

(S)-N-Boc-3-hydroxypiperidine is an important intermediate of the anticancer drug ibrutinib and is mainly synthesized by the asymmetric reduction catalyzed by ketoreductase coupled with glucose dehydrogenase at present. In this study, the coexpression recombinant strains E. coli/pET28-K-rbs-G with single promoter and E. coli/pETDuet-K-G with double promoters were first constructed for the coexpression of ketoreductase and glucose dehydrogenase in the same cell. Then, the catalytic efficiency of E. coli/pET28-K-rbs-G for synthesizing (S)-N-Boc-3-hydroxypiperidine was found to be higher than that of E. coli/pETDuet-K-G due to the more balanced activity ratio and higher catalytic activity. On this basis, the catalytic conditions of E. coli/pET28-K-rbs-G were further optimized, and finally both the conversion of the reaction and the optical purity of the product were higher than 99%. In the end, the cell-free extract was proved to be a better catalyst than the whole cell with the improved catalytic efficiency of different recombinant strains. This study developed a better coexpression strategy for ketoreductase and glucose dehydrogenase by investigating the effect of activity ratios and forms of the biocatalysts on the catalytic efficiency deeply, which provided a research basis for the efficient synthesis of chiral compounds.
Fetal Tissue-Derived Mast Cells (MC) as Experimental Surrogate for In Vivo Connective Tissue MC
Authors: Iuliano Caterina , Absmaier-Kijak Magdalena , Sinnberg Tobias , Hoffard Nils , Hils Miriam , Köberle Martin , Wölbing Florian , Shumilina Ekaterina , Heise Nicole , Fehrenbacher Birgit , Schaller Martin , Lang Florian , Kaesler Susanne , Biedermann Tilo
Published: March 2022

Bone-marrow-derived mast cells are matured from bone marrow cells in medium containing 20% fetal calf serum (FCS), interleukin (IL)-3 and stem-cell factor (SCF) and are used as in vitro models to study mast cells (MC) and their role in health and disease. In vivo, however, BM-derived hematopoietic stem cells account for only a fraction of MC; the majority of MC in vivo are and remain tissue resident. In this study we established a side-by-side culture with BMMC, fetal skin MC (FSMC) or fetal liver MC (FLMC) for comparative studies to identify the best surrogates for mature connective tissue MC (CTMC). All three MC types showed comparable morphology by histology and MC phenotype by flow cytometry. Heterogeneity was detected in the transcriptome with the most differentially expressed genes in FSMC compared to BMMC being Hdc and Tpsb2. Expression of ST2 was highly expressed in BMMC and FSMC and reduced in FLMC, diminishing their secretion of type 2 cytokines. Higher granule content, stronger response to FcεRI activation and significantly higher release of histamine from FSMC compared to FLMC and BMMC indicated differences in MC development in vitro dependent on the tissue of origin. Thus, tissues of origin imprint MC precursor cells to acquire distinct phenotypes and signatures despite identical culture conditions. Fetal-derived MC resemble mature CTMC, with FSMC being the most developed.
Cytochrome c Oxidase Activity as a Metabolic Regulator in Pancreatic Beta-Cells
Authors: Aharon-Hananel Genya , Romero-Afrima Leonor , Saada Ann , Mantzur Carmit , Raz Itamar , Weksler-Zangen Sarah
Published: March 2022

Pancreatic β-cells couple glucose-stimulated insulin secretion (GSIS) with oxidative phosphorylation via cytochrome c oxidase (COX), a mitochondrial respiratory-chain enzyme. The Cohen diabetic-sensitive (CDs) rats exhibit hyperglycemia when fed a diabetogenic diet but maintain normoglycemia on a regular diet. We have previously reported a decreased COX activity in CDs rats and explored its relevance for type 2 diabetes (T2D). In this study, we investigated the relation between COX activity in islets, peripheral-blood mononuclear cells (PBMCs), and GSIS during diabetes development in CDs rats fed a diabetogenic diet for 4, 11, 20, and 30 days and during reversion to normoglycemia in hyperglycemic CDs rats fed a reversion diet for 7, 11, and 20 days. An oral glucose-tolerance test was performed at different periods of the diets measuring blood glucose and insulin concentrations. COX activity was determined in islets and PBMCs isolated from rats at the different periods of the diets. We demonstrated a progressive reduction in COX activity in CDs-islets that correlated positively with the decreasing GSIS (R2 = 0.9691, p < 0.001) and inversely with the elevation in blood glucose levels (R2 = 0.8396, p < 0.001). Hyperglycemia was initiated when islet COX activity decreased below 46%. The reversion diet restored >46% of the islet COX activity and GSIS while re-establishing normoglycemia. Interestingly, COX activity in PBMCs correlated significantly with islet COX activity (R2 = 0.8944, p < 0.001). Our data support islet COX activity as a major metabolic regulator of β-cells function. The correlation between COX activity in PBMCs and islets may serve as a noninvasive biomarker to monitor β-cell dysfunction in diabetes.
Short-Term Functional and Morphological Changes in the Primary Cultures of Trigeminal Ganglion Cells
Authors: Veríssimo Carla Pires , Acosta Filha Lionete Gall , Moreira da Silva Fábio Jorge , Westgarth Harrison , Coelho Aguiar Juliana De Mattos , Pontes Bruno , Moura-Neto Vivaldo , Gazerani Parisa , DosSantos Marcos F.
Published: March 2022

Several studies have proved that glial cells, as well as neurons, play a role in pain pathophysiology. Most of these studies have focused on the contribution of central glial cells (e.g., microglia and astrocytes) to neuropathic pain. Likewise, some works have suggested that peripheral glial cells, particularly satellite glial cells (SGCs), and the crosstalk between these cells and the sensory neurons located in the peripheral ganglia, play a role in the phenomenon that leads to pain. Nonetheless, the study of SGCs may be challenging, as the validity of studying those cells in vitro is still controversial. In this study, a research protocol was developed to examine the potential use of primary mixed neuronal–glia cell cultures obtained from the trigeminal ganglion cells (TGCs) of neonate mice (P10–P12). Primary cultures were established and analyzed at 4 h, 24 h, and 48 h. To this purpose, phase contrast microscopy, immunocytochemistry with antibodies against anti-βIII-tubulin and Sk3, scanning electron microscopy, and time-lapse photography were used. The results indicated the presence of morphological changes in the cultured SGCs obtained from the TGCs. The SGCs exhibited a close relationship with neurons. They presented a round shape in the first 4 h, and a more fusiform shape at 24 h and 48 h of culture. On the other hand, neurons changed from a round shape to a more ramified shape from 4 h to 48 h. Intriguingly, the expression of SK3, a marker of the SGCs, was high in all samples at 4 h, with some cells double-staining for SK3 and βIII-tubulin. The expression of SK3 decreased at 24 h and increased again at 48 h in vitro. These results confirm the high plasticity that the SGCs may acquire in vitro. In this scenario, the authors hypothesize that, at 4 h, a group of the analyzed cells remained undifferentiated and, therefore, were double-stained for SK3 and βIII-tubulin. After 24 h, these cells started to differentiate into SCGs, which was clearer at 48 h in the culture. Mixed neuronal–glial TGC cultures might be implemented as a platform to study the plasticity and crosstalk between primary sensory neurons and SGCs, as well as its implications in the development of chronic orofacial pain.
MoS2-Based Substrates for Surface-Enhanced Raman Scattering: Fundamentals, Progress and Perspective
Authors: Yin Yuan , Li Chen , Yan Yinuo , Xiong Weiwei , Ren Jingke , Luo Wen
Published: March 2022

Surface-enhanced Raman scattering (SERS), as an important tool for interface research, occupies a place in the field of molecular detection and analysis due to its extremely high detection sensitivity and fingerprint characteristics. Substantial efforts have been put into the improvement of the enhancement factor (EF) by way of modifying SERS substrates. Recently, MoS2 has emerged as one of the most promising substrates for SERS, which is also exploited as a complementary platform on the conventional metal SERS substrates to optimize the properties. In this minireview, the fundamentals of MoS2-related SERS are first explicated. Then, the synthesis, advances and applications of MoS2-based substrates are illustrated with special emphasis on their practical applications in food safety, biomedical sensing and environmental monitoring, together with the corresponding challenges. This review is expected to arouse broad interest in nonplasmonic MoS2-related materials along with their mechanisms, and to promote the development of SERS studies.
Biobased Approach for Synthesis of Polymers and Sustainable Formulation of Industrial Hardeners
Authors: Veronico Lorenzo , Andriani Michele , Casiello Michele , Cotugno Pietro , Fusco Caterina , Gentile Luigi , Monopoli Antonio , D’Accolti Lucia
Published: March 2022

The adhesive manufacturing industry needs more eco-sustainable processes. In this regard, the main road is to replace raw fossil materials with renewable resources or waste biomass, and simultaneously improve synthetic steps by using clean and greener reagents under mild conditions. In this paper, a synthetic pathway for producing biobased succinyl peroxide (SP) from waste biomass is reported, and then the application range of this polymerization agent to methacrylates and styrene-free resins is extended. At the same time, new formulations of pastes based on benzoyl or succinyl peroxide, displaying an almost complete biobased carbon content, are investigated and tested as cross-linking agents for mastic marble and unsaturated polyester resins. Physicochemical characterization of the final products and polymers is carried out with thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR), Gel Permeation Chromatography (GPC), Nuclear Magnetic Resonance (NMR) and peak exothermic curve analyses.
An Enhanced Oxidation of Formate on PtNi/Ni Foam Catalyst in an Alkaline Medium
Authors: Nacys Antanas , Šimkūnaitė Dijana , Balčiūnaitė Aldona , Zabielaitė Aušrinė , Upskuvienė Daina , Šebeka Benjaminas , Jasulaitienė Vitalija , Kovalevskij Vitalij , Norkus Eugenijus , Tamašauskaitė-Tamašiūnaitė Loreta
Published: March 2022

In this study, a platinum-coated Ni foam catalyst (denoted PtNi/Ni foam) was investigated for the oxidation of the formate reaction (FOR) in an alkaline medium. The catalyst was fabricated via a two-step procedure, which involved an electroless deposition of the Ni layer using sodium hypophosphite as a reducing agent and the subsequent electrodeposition of the platinum layer. The PtNi/Ni foam catalyst demonstrated enhanced electrocatalytic activity for the FOR in an alkaline medium compared to the Ni/Ni foam catalyst and pure Pt electrode. Moreover, the PtNi/Ni foam catalyst promoted the FOR at more negative potentials than the Pt electrode. This contributed to a significant negative shift in the onset potential, indicating the high activity of the catalyst. Notably, in alkaline media with the PtNi/Ni foam catalyst, the FOR proceeds via a direct pathway mechanism without significant accumulation of poisonous carbonaceous species on the PtNi/Ni foam catalyst.