bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of the Harmony Search Algorithm for Optimization of WDN and Assessment of Pipe Deterioration
Authors: Botella Langa Alejandro , Choi Youn-Gyu , Kim Kwon-Seok , Jang Dong-Woo
Published: April 2022

The relevance of the optimal design of water distribution networks lies in its applicability. Thus, the design must be as efficient and affordable as possible, meaning that it achieves a minimum level of serviceability using an appropriate amount of resources. In this context, an ideal water network requires the use of minimum-size elements to reach the minimum head pressure required for each node of the system with the lowest energy consumption. For this purpose, a relatively new meta-heuristic algorithm, called Harmony Search, was used in this study to optimize the water distribution network of Cheongna International City, Korea, in terms of the pipe diameter. Furthermore, using the same algorithm under a different approach, an assessment of the deterioration of conduits over time was conducted by analyzing the optimal roughness coefficient for the original pipe material, which provides a useful tool for decision making. Other heuristic and meta-heuristic algorithms have been applied to this type of problem; however, Harmony Search provides a convenient implementation at a reasonable computational cost. In this study, Harmony Search is demonstrated to be a valuable tool for water distribution network optimization as well as for pipe aging assessment.
A Preliminary Design and Modeling Analysis of Two-Phase Volumetric Expanders for a Novel Reversible Organic Rankine-Based Cycle for Carnot Battery Technology
Authors: Daniarta Sindu , Kolasiński Piotr , Imre Attila R.
Published: April 2022

Carnot battery technology appears to be a promising solution to increase the development of power generation and offers a good solution for high-capacity, day-to-day energy storage. This technology may utilize the waste heat and store the electricity to recover it later. This article reports the preliminary analysis of a specially designed Carnot battery configuration employing a novel reversible Rankine-based thermodynamic cycle (RRTC). In this case, one volumetric expander is not only installed to generate power from a heat engine, but also to recover power during heat pump operating mode. The preliminary design and modeling results were obtained based on calculations taken from working fluid thermal properties of propane with some specific boundary conditions (i.e., secondary fluid hot temperature of 348.15 K, cooling temperature of 228.15 K, and waste heat temperature of 338.15 K). The results show that isentropic efficiency, pressure, and volumetric expansion ratio from both heat engine and heat pump operating modes are important parameters that must be taken into account when designing the two-phase expander for RRTC. The obtained results show that a designed two-phase volumetric expander in RRTC features a pressure ratio of 2.55 ± 1.15 and a volumetric ratio of 0.21 ± 0.105, and the Carnot battery may achieve the performance of 0.50–0.98.
Uncontrolled and Controlled Destruction of Acetylene Pressure Cylinders
Authors: Jankůj Vojtěch , Mynarz Miroslav , Lepík Petr
Published: April 2022

The risk of physical destruction of a pressure cylinder increases with increasing temperature load. Acetylene is a significant hazardous gas in cylinders. If the destruction pressure is reached, the cylinder is torn into two or more pieces, followed by the gas release to the environment. The presence of ignition sources could lead to the formation of the fireball phenomenon. This phenomenon generally reaches approximately ten meters in diameter and can be accompanied by a blast wave and a spread of the cylinder’s fragments or surrounding objects. The consequences of this type of fire could be fatal. Shooting through the cylinder shell may lead to the mitigation, if not elimination, of the effects of the uncontrolled destruction mentioned above. As a result of the review of relevant publications, several states commonly use this method as a standard procedure. Internal gas is released through the resulting hole, the pressure drops down and the gas creates a fire jet or disperses to surroundings. This study is based on a large-scale experiment where acetylene cylinders were placed inside a prepared woodpile and exposed to fire. In the fire condition, the cylinders exploded and created fireballs, or were penetrated via shooting and created fire jets. The results from these tests were compared, focusing on the shooting procedure, then analyzed and discussed.
A Physical Mechanism for the Indian Summer Monsoon—Arctic Sea-Ice Teleconnection
Authors: Sundaram Suchithra , Holland David M.
Published: April 2022

Significant changes in the Arctic climate, particularly a rapid decline of September Arctic sea ice has occurred over the past few decades. Though the exact reason for such drastic changes is still unknown, studies suggest anthropogenic drivers, natural variability of the climate system, and a combination of both as reasons. The present study focus on the influence of one of the natural variabilities of the climate system, the teleconnections associated with the Indian Summer Monsoon (ISM), and its relationship to September Arctic sea ice. Using 50 years (1951–2000) of National Center for Environmental Prediction (NCEP)/National Center for Atmospheric Research (NCAR) NCEP/NCAR reanalysis data, APHRODITE precipitation data, Gridded Monthly Sea Ice Extent and Concentration, 1850 Onward, V2, and HadISST sea-ice concentration data, it is shown that during many strong (weak) ISM years, the Arctic sea ice increased (decreased) predominantly over the Chukchi and Beaufort Seas. The ISM plays a significant role in causing a positive (negative) North Atlantic Oscillation (NAO) during strong (weak) ISM years through the monsoon-desert mechanism associated with monsoonal heating. Simultaneously, the NAO during a strong (weak) ISM causes weakening (strengthening) of the Beaufort Sea High (BSH). The strength of the BSH modulates the Arctic atmospheric circulation, advecting cold air and the direction of the transpolar drift stream, both leading to the generation of more (less) sea ice over the Chukchi-Beaufort Sea region during strong (weak) ISM years. The study illustrates a new atmospheric teleconnection between the tropics and the Arctic.
A Regression-Based Technique for Capacity Estimation of Lithium-Ion Batteries
Authors: Madani Seyed , Soghrati Raziye , Ziebert Carlos
Published: April 2022

Electric vehicles (EVs) and hybrid vehicles (HEVs) are being increasingly utilized for various reasons. The main reasons for their implementation are that they consume less or do not consume fossil fuel (no carbon dioxide pollution) and do not cause sound pollution. However, this technology has some challenges, including complex and troublesome accurate state of health estimation, which is affected by different factors. According to the increase in electric and hybrid vehicles’ application, it is crucial to have a more accurate and reliable estimation of state of charge (SOC) and state of health (SOH) in different environmental conditions. This allows improving battery management system operation for optimal utilization of a battery pack in various operating conditions. This article proposes an approach to estimate battery capacity based on two parameters. First, a practical and straightforward method is introduced to assess the battery’s internal resistance, which is directly related to the battery’s remaining useful life. Second, the different least square algorithm is explored. Finally, a promising, practical, simple, accurate, and reliable technique is proposed to estimate battery capacity appropriately. The root mean square percentage error and the mean absolute percentage error of the proposed methods were calculated and were less than 0.02%. It was concluded the geometry method has all the advantages of a recursive manner, including a fading memory, a close form of a solution, and being applicable in embedded systems.
AmazonForest: In Silico Metaprediction of Pathogenic Variants
Authors: Palheta Helber Gonzales Almeida , Gonçalves Wanderson Gonçalves , Brito Leonardo Miranda , dos Santos Arthur Ribeiro , dos Reis Matsumoto Marlon , Ribeiro-dos-Santos Ândrea , de Araújo Gilderlanio Santana
Published: April 2022

ClinVar is a web platform that stores ∼789,000 genetic associations with complex diseases. A partial set of these cataloged genetic associations has challenged clinicians and geneticists, often leading to conflicting interpretations or uncertain clinical impact significance. In this study, we addressed the (re)classification of genetic variants by AmazonForest, which is a random-forest-based pathogenicity metaprediction model that works by combining functional impact data from eight prediction tools. We evaluated the performance of representation learning algorithms such as autoencoders to propose a better strategy. All metaprediction models were trained with ClinVar data, and genetic variants were annotated with eight functional impact predictors cataloged with SnpEff/SnpSift. AmazonForest implements the best random forest model with a one hot data-encoding strategy, which shows an Area Under ROC Curve of ≥0.93. AmazonForest was employed for pathogenicity prediction of a set of ∼101,000 genetic variants of uncertain significance or conflict of interpretation. Our findings revealed ∼24,000 variants with high pathogenic probability (RFprob≥0.9). In addition, we show results for Alzheimer’s Disease as a demonstration of its application in clinical interpretation of genetic variants in complex diseases. Lastly, AmazonForest is available as a web tool and R object that can be loaded to perform pathogenicity predictions.
Testosterone as a Biomarker of Adverse Clinical Outcomes in SARS-CoV-2 Pneumonia
Authors: Marinelli Lorenzo , Beccuti Guglielmo , Zavattaro Marco , Cagnina Serena , Gesmundo Iacopo , Bona Chiara , Lopez Chiara , Scabini Silvia , Canta Francesca , Mornese Pinna Simone , Lupia Tommaso , Di Bisceglie Cataldo , Ponzetto Federico , Settanni Fabio , De Rosa Francesco Giuseppe , Ghigo Ezio , Motta Giovanna
Published: April 2022

Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may affect testicles. Lower testosterone levels have been associated with worse clinical outcomes and higher mortality. Our objective was to evaluate the hypothalamic–pituitary–gonadal axis of men admitted with SARS-CoV-2 pneumonia and its link with the pneumonia-treatment intensification. Short-term changes in hormonal parameters were also assessed. Methods: Men admitted with SARS-CoV-2 pneumonia were recruited in two different hospitals in Piedmont, Italy. In all patients, the assessment of total testosterone (TT), calculated free testosterone (cFT), gonadotropins, inhibin B (InhB), and other biochemical evaluations were performed at admission (T0) and before discharge (T1). Through a review of medical records, clinical history was recorded, including data on pneumonia severity. Results: Thirty-five men (median age 64 [58–74] years) were recruited. Lower TT and cFT levels at T0 were associated with CPAP therapy (p = 0.045 and 0.028, respectively), even after adjusting for age and PaO2/FIO2 ratio in a multivariable analysis. In those discharged alive, lower TT and cFT levels were associated with longer hospital stay (p < 0.01). TT, cFT, and InhB were below the normal range at T0 and significantly increased at T1 (TT 1.98 [1.30–2.72] vs. 2.53 [1.28–3.37] ng/mL, p = 0.038; cFT (0.0441 [0.0256–0.0742] vs. 0.0702 [0.0314–0.0778] ng/mL, p = 0.046; InhB 60.75 [25.35–88.02] vs. 77.05 [51.15–134.50], p < 0.01). Conclusions: Both TT and cFT levels are associated with adverse clinical outcomes in men admitted with SARS-CoV-2 pneumonia. As TT, cFT and InhB levels increase before discharge, short-term functional recovery of steroidogenesis and an indirect improvement of spermatozoa functional status could be hypothesized.
Modulating the Antioxidant Response for Better Oxidative Stress-Inducing Therapies: How to Take Advantage of Two Sides of the Same Medal?
Authors: Shaw Priyanka , Kumar Naresh , Sahun Maxime , Smits Evelien , Bogaerts Annemie , Privat-Maldonado Angela
Published: April 2022

Oxidative stress-inducing therapies are characterized as a specific treatment that involves the production of reactive oxygen and nitrogen species (RONS) by external or internal sources. To protect cells against oxidative stress, cells have evolved a strong antioxidant defense system to either prevent RONS formation or scavenge them. The maintenance of the redox balance ensures signal transduction, development, cell proliferation, regulation of the mechanisms of cell death, among others. Oxidative stress can beneficially be used to treat several diseases such as neurodegenerative disorders, heart disease, cancer, and other diseases by regulating the antioxidant system. Understanding the mechanisms of various endogenous antioxidant systems can increase the therapeutic efficacy of oxidative stress-based therapies, leading to clinical success in medical treatment. This review deals with the recent novel findings of various cellular endogenous antioxidant responses behind oxidative stress, highlighting their implication in various human diseases, such as ulcers, skin pathologies, oncology, and viral infections such as SARS-CoV-2.
Dysregulation of lncRNA–miRNA–mRNA Interactome as a Marker of Metastatic Process in Ovarian Cancer
Authors: Pronina Irina V. , Uroshlev Leonid A. , Moskovtsev Alexey A. , Zaichenko Danila M. , Filippova Elena A. , Fridman Marina V. , Burdennyy Alexey M. , Loginov Vitaly I. , Kazubskaya Tatiana P. , Kushlinskii Nikolay E. , Dmitriev Alexey A. , Braga Eleonora A. , Brovkina Olga I.
Published: April 2022

Ovarian cancer (OC) is one of the most common types of cancer among malignancies of the female reproductive system. This pathology is asymptomatic until advanced stages and has a poor prognosis. Our study aimed to search for lncRNA–miRNA–mRNA competing triplets that promote ovarian tumorigenesis. For this purpose, we analyzed tumor samples from the TCGA database and verified the results experimentally in a set of 46 paired samples of tumor and matched histologically unchanged ovarian tissues from OC patients. The list of RNAs selected in silico for experimental studies included 13 mRNAs, 10 lncRNAs, and 5 miRNAs related to epithelial–mesenchymal transition and angiogenesis. We evaluated the expression of these RNAs by qRT-PCR and assessed the correlation between levels of miRNAs, mRNAs, and lncRNAs. Sixteen significant triplets were revealed, in some of which, e.g., OIP5-AS1–miR-203a–c-MET and OIP5-AS1–miR-203a–ZEB2, both lncRNA and mRNA had sites for miR-203a direct binding. Transfection of the OVCAR-3 and SKOV-3 cell lines with the miR-203a mimic was used to confirm the novel links of miR-203a with ZEB2 and c-MET in OC. These connections suggest that the interactomes have the potential for diagnostics of metastasis at early onset.
A Novel Anti-CD73 Antibody That Selectively Inhibits Membrane CD73 Shows Antitumor Activity and Induces Tumor Immune Escape
Authors: Kellner Markus , von Neubeck Bettina , Czogalla Bastian , Feederle Regina , Vick Binje , Jeremias Irmela , Zeidler Reinhard
Published: April 2022

CD73 catalyzes the conversion of ATP to adenosine, which is involved in various physiological and pathological processes, including tumor immune escape. Because CD73 expression and activity are particularly high on cancer cells and contribute to the immunosuppressive properties of the tumor environment, it is considered an attractive target molecule for specific cancer therapies. In line, several studies demonstrated that CD73 inhibition has a significant antitumor effect. However, complete blocking of CD73 activity can evoke autoimmune phenomena and adverse side effects. We developed a CD73-specific antibody, 22E6, that specifically inhibits the enzymatic activity of membrane-tethered CD73 present in high concentrations on cancer cells and cancer cell-derived extracellular vesicles but has no inhibitory effect on soluble CD73. Inhibition of CD73 on tumor cells with 22E6 resulted in multiple effects on tumor cells in vitro, including increased apoptosis and interference with chemoresistance. Intriguingly, in a xenograft mouse model of acute lymphocytic leukemia (ALL), 22E6 treatment resulted in an initial tumor growth delay in some animals, followed by a complete loss of CD73 expression on ALL cells in all 22E6 treated animals, indicating tumor immune escape. Taken together, 22E6 shows great potential for cancer therapy, favorably in combination with other drugs.
Visualising the Emerging Platform of Using Microalgae as a Sustainable Bio-Factory for Healthy Lipid Production through Biocompatible AIE Probes
Authors: Reza AHM Mohsinul , Rakhi Sharmin Ferdewsi , Zhu Xiaochen , Tang Youhong , Qin Jianguang
Published: April 2022

Nowadays, a particular focus is using microalgae to get high-valued health beneficiary lipids. The precise localisation of the lipid droplets (LDs) and biochemical changes are crucial to portray the lipid production strategy in algae, but it requires an in vivo tool to rapidly visualise LD distribution. As a novel strategy, this study focuses on detecting lipid bioaccumulation in a green microalga, Chlamydomonas reinhardtii using the aggregation-induced emission (AIE) based probe, 2-DPAN (C24H18N2O). As the messenger molecule and stress biomarker, hydrogen peroxide (H2O2) activity was detected in lipid synthesis with the AIE probe, TPE-BO (C38H42B2O4). Distinctive LDs labelled with 2-DPAN have elucidated the lipid inducing conditions, where more health beneficiary α-linolenic acid has been produced. TPE-BO labelled H2O2 have clarified the involvement of H2O2 during lipid biogenesis. The co-staining procedure with traditional green BODIPY dye and red chlorophyll indicates that 2-DPAN is suitable for multicolour LD imaging. Compared with BODIPY, 2-DPAN was an efficient sample preparation technique without the washing procedure. Thus, 2-DPAN could improve traditional fluorescent probes currently used for lipid imaging. In addition, the rapid, wash-free, multicolour AIE-based in vivo probe in the study of LDs with 2-DPAN could advance the research of lipid production in microalgae.
Relative Water Economy Is a Useful Index of Aridity Tolerance for Australian Poephiline Finches
Authors: Withers Philip C. , Cooper Christine E. , Larcombe Alexander N.
Published: April 2022

We evaluate if the iconic Australian Zebra Finch (Taeniopygia guttata) has a unique physiology or if its metabolic, thermal and hygric physiology are similar to other Australian poephiline finches, by comparing it with three other species, the arid-habitat Painted Finch (Emblema pictum) and the mesic-habitat Double-barred (Taeniopygia bichenovii) and Red-browed (Neochmia temporalis) Finches. All physiological variables responded to ambient temperature as expected. There were no species differences for any of the standard physiological variables, consistent with the hypotheses that birds are pre-adapted to arid habitats, the recent development of Australian deserts has limited opportunity for physiological adaptation, and all four species share similar behavioural and ecological traits. Nevertheless, the ambient temperature where metabolic water production equals evaporative water loss (point of relative water economy) was highest for the Zebra (19.1 °C), lower for Double-barred (16.4 °C) and Painted (15.2 °C) and lowest for Red-Browed (4.1 °C) Finches, corresponding with their general patterns of habitat aridity. The point of relative water economy may be a sensitive index for assessing a species’ tolerance of aridity because it integrates individual physiological variables. We conclude that the Zebra Finch is not a physiological outlier amongst Australian finches, but is at the end of a continuum of aridity tolerance for the four study species.
Features and Extra-Striate Body Area Representations of Diagnostic Body Parts in Anger and Fear Perception
Authors: Ren Jie , Ding Rui , Li Shuaixia , Zhang Mingming , Wei Dongtao , Feng Chunliang , Xu Pengfei , Luo Wenbo
Published: April 2022

Social species perceive emotion via extracting diagnostic features of body movements. Although extensive studies have contributed to knowledge on how the entire body is used as context for decoding bodily expression, we know little about whether specific body parts (e.g., arms and legs) transmit enough information for body understanding. In this study, we performed behavioral experiments using the Bubbles paradigm on static body images to directly explore diagnostic body parts for categorizing angry, fearful and neutral expressions. Results showed that subjects recognized emotional bodies through diagnostic features from the torso with arms. We then conducted a follow-up functional magnetic resonance imaging (fMRI) experiment on body part images to examine whether diagnostic parts modulated body-related brain activity and corresponding neural representations. We found greater activations of the extra-striate body area (EBA) in response to both anger and fear than neutral for the torso and arms. Representational similarity analysis showed that neural patterns of the EBA distinguished different bodily expressions. Furthermore, the torso with arms and whole body had higher similarities in EBA representations relative to the legs and whole body, and to the head and whole body. Taken together, these results indicate that diagnostic body parts (i.e., torso with arms) can communicate bodily expression in a detectable manner.
Behavioral Phenotype in Heterozygous DAT Rats: Transgenerational Transmission of Maternal Impact and the Role of Genetic Asset
Authors: Manoni Greta , Puzzo Concetto , Gigantesco Antonella , Adriani Walter
Published: April 2022

Dopamine transporter (DAT) is involved in dopamine (DA) reuptake in presynaptic terminals. Deletion of DAT results in a hyperdopaminergic KO-rat phenotype. To conduct our studies in heterozygous DAT rats, several pedigree lines were created, with known derivation of the allele (i.e., maternal or paternal). Our purpose was to elucidate the role of parental origin rather than maternal care, assessing if maternal maltreatments generated sequelae in female offspring. In the first experiment, female rats and their pups were observed during postnatal lactation. Control dams were WT and heterozygous ones were MAT (but K-MAT, with previous experience of early maltreatment by their KO adoptive dams). WT dams were highly attracted to their offspring (predictably, they spent a lot of time licking their pups); in contrast, K-MAT dams showed strangely comparable levels of caring for their pups and exploring the environment. Subsequently, peculiar features of the circadian cycle were found in adolescent rats with different epigenotypes (WT, MUX = offspring of MAT father, MIK = offspring of K-MAT dam). The MIK epigenotype produced locomotor hyperactivity also during resting hours, well above typical values. The MUX epigenotype, on the other hand, was less active and presented a depression-like profile. This study is unique: maltreatment was generated in a spontaneous way from a DAT-KO mother to offspring. We highlight how future studies will address separate contributions by genotype and upbringing. In conclusion, paternal-allele asset generates sequelae diametrically opposed to the inheritance of early maternal trauma.
Valorization of Dredged Sediments in Manufacturing Compressed Earth Blocks Stabilized by Alkali-Activated Fly Ash Binder
Authors: Brahim Mohamedou , Ndiaye Khadim , Aggoun Salima , Maherzi Walid
Published: April 2022

The valorization of dredged sediments is a promising solution to reduce the strain on natural resources, which is in line with sustainable development goals. This study aims to evaluate the potential valorization of dredged sediment in manufacturing compressed earth blocks (CEBs). The CEBs were stabilized by a combination of fly ash (FA) with sodium hydroxide (NaOH). The stabilization was achieved by partial substitution of sediment for fly ash with six different percentages 10, 20, 30, 40, and 50% by weight. The CEBs samples were characterized in terms of structural, microstructural, mechanical, and thermal properties. The results showed that increasing FA content significantly improves the mechanical strength of CEBs, dry compressive strength ranges from 2.47 MPa to 9 MPa, whereas wet compressive strength ranges from 0.95 MPa to 6.9 MPa. The mechanical performance is related to the amount of alkali-activated fly ash gels, which bind the sediment grains and makes the CEBs more compact and resistant. The optimal dosage of alkali-activated fly ash to replace the sediment was between 10 and 20%. In this substitution range, mechanical performance and physical properties improved significantly. In addition, the thermal properties varied slightly with alkali-activated FA content.
Spatiotemporal Analysis of Influencing Factors of Carbon Emission in Public Buildings in China
Authors: Du Zhuoqun , Liu Yisheng , Zhang Zhidong
Published: April 2022

The rapid development of public buildings has greatly increased the country’s energy consumption and carbon emissions. Excessive carbon emissions contribute to global warming. This paper aims to measure the carbon emissions in the operation of public buildings, and to identify the multiple influencing factors of carbon emissions in operational public buildings. First, the spatial and temporal variation characteristics of carbon emissions from public buildings in 30 provinces of China from 2008–2019 are analyzed. Second, a green building index is constructed, and the STIRPAT (Stochastic Impacts by Regression on Population, Affluence, and Technology) model is utilized to explore the relationship between each influencing factor and carbon emissions, using spatial and temporal geographically weighted regression analysis. The results show that the effects of population, urbanization rate, GDP per capita, green building index, and industrial structure on carbon emissions from public buildings all show spatial correlation and differences. There are east-west differences in the operational carbon emissions of public buildings in China’s provinces. Cluster evolution shows a spatially increasing trend from west to east. To some extent, policymakers can develop appropriate policies for different provinces through the findings.
PSMA and Choline PET for the Assessment of Response to Therapy and Survival Outcomes in Prostate Cancer Patients: A Systematic Review from the Literature
Authors: Alongi Pierpaolo , Laudicella Riccardo , Lanzafame Helena , Farolfi Andrea , Mapelli Paola , Picchio Maria , Burger Irene A. , Iagaru Andrei , Minutoli Fabio , Evangelista Laura
Published: April 2022

The aims of this systematic review were to (1) assess the utility of PSMA-PET and choline-PET in the assessment of response to systemic and local therapy, and to (2) determine the value of both tracers for the prediction of response to therapy and survival outcomes in prostate cancer. We performed a systematic literature search in PubMed/Scopus/Google Scholar/Cochrane/EMBASE databases (between January 2010 and October 2021) accordingly. The quality of the included studies was evaluated following the “Quality Assessment of Prognostic Accuracy Studies” tool (QUAPAS-2). We selected 40 articles: 23 articles discussed the use of PET imaging with [68Ga]PSMA-11 (16 articles/1123 patients) or [11C]/[18F]Choline (7 articles/356 patients) for the prediction of response to radiotherapy (RT) and survival outcomes. Seven articles (three with [68Ga]PSMA-11, three with [11C]Choline, one with [18F]Choline) assessed the role of PET imaging in the evaluation of response to docetaxel (as neoadjuvant therapy in one study, as first-line therapy in five studies, and as a palliative regimen in one study). Seven papers with radiolabeled [18F]Choline PET/CT (n = 121 patients) and three with [68Ga]PSMA-11 PET (n = 87 patients) were selected before and after enzalutamide/abiraterone acetate. Finally, [18F]Choline and [68Ga]PSMA-11 PET/CT as gatekeepers for the treatment of metastatic prostate cancer with Radium-223 were assessed in three papers. In conclusion, in patients undergoing RT, radiolabeled choline and [68Ga]PSMA-11 have an important prognostic role. In the case of systemic therapies, the role of such new-generation imaging techniques is still controversial without sufficient data, thus requiring additional in this scenario.
Care Management and Survival of Patients Diagnosed with Synchronous Metastatic Colorectal Cancer: A High-Resolution Population-Based Study in Two French Areas
Authors: Mulliri Andrea , Gardy Joséphine , Cariou Mélanie , Launoy Guy , Robaszkiewicz Michel , Alves Arnaud , Bouvier Véronique , Dejardin Olivier
Published: April 2022

Population-based studies provide the opportunity to assess the real-world applicability of current clinical practices. The present research evaluated the survival outcomes of different therapeutic strategies for colorectal cancer (CRC) with synchronous metastasis (SM). The differential impact of treatment sequence, viz. whether chemotherapy (CT) or primary tumor resection (PTR) was performed first, was also evaluated. Methods: All CRC cases with SM diagnosed between 2006 and 2016 (N = 3062) were selected from two specialized digestive cancer registries from northwest France. Cox regression analysis was used to assess survival. Multivariable logistic regression was used to examine factors related to the combination of PTR and CT. Results: The longest survival was observed in patients treated by PTR combined with CT (Group 4; N = 1159). Overall survival was 51.80% at one year (95% Confidence Interval (CI) 50.00–53.60%) and 9.40% at five years (95% CI, 8.30–10.60%). Survival did not differ with respect to the order of treatment in multivariable analysis (hazard ratio, 1.05; 95% CI, 0.88–1.24; p = 0.55). Conclusion: Regardless of the sequence of treatment, a PTR + CT offered the best survival in patients with CRC and SM, even though few were eligible for combination therapy (38%).
A Review of Nuclear Medicine Approaches in the Diagnosis and the Treatment of Gynecological Malignancies
Authors: Vahidfar Nasim , Farzanefar Saeed , Ahmadzadehfar Hojjat , Molloy Eóin N. , Eppard Elisabeth
Published: April 2022

Nuclear medicine is defined as the diagnosis and the treatment of disease using radiolabeled compounds known as radiopharmaceuticals. Single-photon emission computed tomography/computed tomography (SPECT/CT) and positron emission tomography/computer tomography (PET/CT) based radiopharmaceuticals have proven reliable in diagnostic imaging in nuclear medicine and cancer treatment. One of the most critical cancers that also relies on an early diagnosis is gynecological cancer. Given that approximately 25% of all cancers in developing countries are a subset of gynecological cancer, investigating this cancer subtype is of significant clinical worth, particularly in light of its high rate of mortality. With accurate identification of high grade distant abdominal endometrial cancer as well as extra abdominal metastases, 18F-Fluorodeoxyglucose ([18F]FDG) PET/CT imaging is considered a valuable step forward in the investigation of gynecological cancer. Considering these factors, [18F]FDG PET/CT imaging can assist in making management of patient therapy more feasible. In this literature review, we will provide a short overview of the role of nuclear medicine in the diagnosis of obstetric and gynecological cancers.
The Influence of Motion on the Delivery Accuracy When Comparing Actively Scanned Carbon Ions versus Protons at a Synchrotron-Based Radiotherapy Facility
Authors: Lebbink Franciska , Stock Markus , Georg Dietmar , Knäusl Barbara
Published: April 2022

Motion amplitudes, in need of mitigation for moving targets irradiated with pulsed carbon ions and protons, were identified to guide the decision on treatment and motion mitigation strategy. Measurements with PinPoint ionisation chambers positioned in an anthropomorphic breathing phantom were acquired to investigate different tumour motion scenarios, including rib and lung movements. The effect of beam delivery dynamics and spot characteristics was considered. The dose in the tumour centre was deteriorated up to 10% for carbon ions but only up to 5% for protons. Dose deviations in the penumbra increased by a factor of two when comparing carbon ions to protons, ranging from 2 to 30% for an increasing motion amplitude that was strongly dependent on the beam intensity. Layer rescanning was able to diminish the dose distortion caused by tumour motion, but an increase in spot size could reduce it even further to 5% within the target and 10% at the penumbra. An increased need for motion mitigation of carbon ions compared to protons was identified to assure target coverage and sparing of adjacent organs at risk in the penumbra region and outside the target. For the clinical implementation of moving target treatments at a synchrotron-based particle facility complex, time dependencies needed to be considered.
Ni-Cu/Al2O3 from Layered Double Hydroxides Hydrogenates Furfural to Alcohols
Authors: Aldureid Abdulaziz , Medina Francisco , Patience Gregory S. , Montané Daniel
Published: April 2022

The hydrogenation of furfural is an important process in the synthesis of bio-based chemicals. Copper-based catalysts favor the hydrogenation of furfural to alcohols. Catalytic activity and stability were higher at a Ni-to-Cu atomic ratio of 1:1 and 1.5:0.5 compared to 0.5:1.5. Here, we prepared Ni-Cu/Al2O3 hydrogenation catalysts derived from layered double hydroxides (LDHs). Catalysts calcined at 673 K and reduced at 773 K with nominal Ni/Cu atomic ratios y/x = 1.5/0.5, 1/1 and 0.5/1.5 were characterized by XRD, FESEM-EDX, H2-TPR, XPS, FAA and BET. Their activity was tested at 463 K and in a 0.05 g g−1 furfural solution in ethanol, and the space velocity in a packed-bed reactor (PBR) was 2.85 gFF gcat−1 h−1. In a slurry reactor (SSR) at 5 MPa H2 and a contact time of 4 h, conversion was complete, while it varied from 91 to 99% in the PBR. Tetrahydrofurfuryl alcohol (TFA) was the main product in the SSR, with a selectivity of 32%, 63% and 56% for Ni0.5Cu1.5Al1, Ni1Cu1Al1 and Ni1.5Cu0.5Al1, respectively. The main product in the atmospheric PBR was furfuryl alcohol (FA), with a selectivity of 57% (Ni0.5Cu1.5Al1), 61% (Ni1Cu1Al1) and 58% (Ni1.5Cu0.5Al1). Other products included furan, methylfuran, 1-butanol and 1,2-pentanediol. Ethyl tetrahydrofurfuryl ether and difurfuryl ether were also formed via the nucleophilic addition of furfural with ethanol and furfuryl alcohol.
The Sin3A/MAD1 Complex, through Its PAH2 Domain, Acts as a Second Repressor of Retinoic Acid Receptor Beta Expression in Breast Cancer Cells
Authors: Dahiya Nisha Rani , Leibovitch Boris A. , Kadamb Rama , Bansal Nidhi , Waxman Samuel
Published: April 2022

Retinoids are essential in balancing proliferation, differentiation and apoptosis, and they exert their effects through retinoic acid receptors (RARs) and retinoid X receptors (RXRs). RARβ is a tumor-suppressor gene silenced by epigenetic mechanisms such as DNA methylation in breast, cervical and non-small cell lung cancers. An increased expression of RARβ has been associated with improved breast cancer-specific survival. The PAH2 domain of the scaffold protein SIN3A interacts with the specific Sin3 Interaction Domain (SID) of several transcription factors, such as MAD1, bringing chromatin-modifying proteins such as histone deacetylases, and it targets chromatin for specific modifications. Previously, we have established that blocking the PAH2-mediated Sin3A interaction with SID-containing proteins using SID peptides or small molecule inhibitors (SMI) increased RARβ expression and induced retinoic acid metabolism in breast cancer cells, both in in vitro and in vivo models. Here, we report studies designed to understand the mechanistic basis of RARβ induction and function. Using human breast cancer cells transfected with MAD1 SID or treated with the MAD SID peptide, we observed a dissociation of MAD1, RARα and RARβ from Sin3A in a coimmunoprecipitation assay. This was associated with increased RARα and RARβ expression and function by a luciferase assay, which was enhanced by the addition of AM580, a specific RARα agonist; EMSA showed that MAD1 binds to E-Box, similar to MYC, on the RARβ promoter, which showed a reduced enrichment of Sin3A and HDAC1 by ChIP and was required for the AM580-enhanced RARβ activation in MAD1/SID cells. These data suggest that the Sin3A/HDAC1/2 complex co-operates with the classical repressors in regulating RARβ expression. These data suggest that SIN3A/MAD1 acts as a second RARβ repressor and may be involved in fine-tuning retinoid sensitivity.
Comparative Proteomic Profiling of Ectosomes Derived from Thyroid Carcinoma and Normal Thyroid Cells Uncovers Multiple Proteins with Functional Implications in Cancer
Authors: Surman Magdalena , Kędracka-Krok Sylwia , Wilczak Magdalena , Rybczyński Piotr , Jankowska Urszula , Przybyło Małgorzata
Published: April 2022

Proteins carried by tumor-derived ectosomes play an important role in cancer progression, and are considered promising diagnostic markers. In the present study, a shotgun nanoLC–MS/MS proteomic approach was applied to profile and compare the protein content of ectosomes released in vitro by normal human thyroid follicular epithelial Nthy-ori 3-1 cells and human anaplastic thyroid carcinoma (TC) 8305C cells. Additionally, the pro-migratory and pro-proliferative effects of Nthy-ori 3-1- and 8305C-derived ectosomes exerted on the recipient cells were assessed in wound closure and Alamar Blue assays. A total of 919 proteins were identified in all replicates of 8305C-derived ectosomes, while Nthy-ori 3-1-derived ectosomes contained a significantly lower number of 420 identified proteins. Qualitative analysis revealed 568 proteins present uniquely in 8305C-derived ectosomes, suggesting their applicability in TC diagnosis and management. In addition, 8305C-derived ectosomes were able to increase the proliferation and motility rates of the recipient cells, likely due to the ectosomal transfer of the identified cancer-promoting molecules. Our description of ectosome protein content and its related functions provides the first insight into the role of ectosomes in TC development and progression. The results also indicate the applicability of some of these ectosomal proteins for further investigation regarding their potential as circulating TC biomarkers.
Effect of Carrier Gas Flow Rate on the Morphology and Luminescence Properties of CsPbBr3 Microcrystals
Authors: Wang Xiaoting , You Fangfang , Huang Jianping , Yao Yi , Xu Faqiang
Published: April 2022

All-inorganic halide perovskites, especially lead perovskite microcrystals, have attracted more and more attention because of their excellent photoelectric properties and chemical stability. Herein, high quality CsPbBr3 microcrystals with three different stable morphologies, namely microplate, frustum of a square pyramid and pyramid, were synthesized by the chemical vapor deposition (CVD) method through altering the flow rate of a carrier gas and were comparatively studied in structure and optical property. The photoluminescence (PL) results showed that the CsPbBr3 microplate has the best luminescence property. The structural characterization results by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), X-ray rocking curves (XRC) and Raman revealed that the flow rate of the carrier gas could manipulate the morphology evolution of CsPbBr3 microcrystals and further impact their luminescence properties.
Combinatorial Power of cfDNA, CTCs and EVs in Oncology
Authors: Keup Corinna , Kimmig Rainer , Kasimir-Bauer Sabine
Published: April 2022

Liquid biopsy is a promising technique for clinical management of oncological patients. The diversity of analytes circulating in the blood useable for liquid biopsy testing is enormous. Circulating tumor cells (CTCs), cell-free DNA (cfDNA) and extracellular vesicles (EVs), as well as blood cells and other soluble components in the plasma, were shown as liquid biopsy analytes. A few studies directly comparing two liquid biopsy analytes showed a benefit of one analyte over the other, while most authors concluded the benefit of the additional analyte. Only three years ago, the first studies to examine the value of a characterization of more than two liquid biopsy analytes from the same sample were conducted. We attempt to reflect on the recent development of multimodal liquid biopsy testing in this review. Although the analytes and clinical purposes of the published multimodal studies differed significantly, the additive value of the analytes was concluded in almost all projects. Thus, the blood components, as liquid biopsy reservoirs, are complementary rather than competitive, and orthogonal data sets were even shown to harbor synergistic effects. The unmistakable potential of multimodal liquid biopsy testing, however, is dampened by its clinical utility, which is yet to be proven, the lack of methodical standardization and insufficiently mature reimbursement, logistics and data handling.
Prenatal Diagnosis and Outcome of Umbilical–Portal–Systemic Venous Shunts: Experience of a Tertiary Center and Proposal for a New Complex Type
Authors: Nagy Rodica Daniela , Iliescu Dominic Gabriel
Published: April 2022

Aims: To share our experience in the prenatal diagnosis of umbilical–portal–systemic venous shunts (UPSVS) and to study the prognostic factors for proper prenatal and perinatal management. Material and Methods: A five-year prospective study regarding the detection of UPSVS was conducted in two referral centers, Medgin Ginecho Clinic and the Prenatal Diagnostic Unit of the tertiary center, University Emergency County Hospital Craiova, Romania. We included in the analysis a series of agenesis of ductus venosus (ADV) cases previously reported by our center. We analyzed the incidence of the UPSVS types, their associations, and outcome predictors. Results: UPSVS were diagnosed in all 16 cases that were presented to our center at the time of first trimester anomaly scan, except one (94.12%). We diagnosed: 19 type I (61.2%), 4 type II (12.9%) and 5 type IIIa (16.1%) UPSVS. In three cases (9.6%) we noted multiple shunts, which we referred to as type IV (a new UPSVS type). Type IIIa-associated fetal growth restriction (FGR) was found in 60% of cases. Major anomalies worsened the outcome. Of the UPVSS cases, 57.1% were associated with PVS anomalies. Genetic anomalies were present in 40% of the tested cases. Conclusions: The incidence of UPSVS in our study was 0.2%. Early detection is feasible. The postnatal outcome mainly depends on the presence of structural, genetic and PVS anomalies. FGR may be associated. The new category presented a poor outcome secondary to poor hemodynamic and major associated anomalies.
Differential Vaginal Microbiota Profiling in Lactic-Acid-Producing Bacteria between Infertile Women with and without Chronic Endometritis
Authors: Tanaka Suguru , Sakuraba Yoshiyuki , Kitaya Kotaro , Ishikawa Tomomoto
Published: April 2022

Purpose: Chronic endometritis (CE) is an infectious and inflammatory disorder associated with infertility of unknown etiology, repeated implantation failure, and recurrent pregnancy loss. In the current clinical practice, intrauterine interventions such as endometrial biopsy/histopathologic examinations and/or hysteroscopy are required for the diagnosis of CE. In this study, we analyzed the microbiota in vaginal secretions (VS) as a potential prediction tool for CE in infertile women. Methods: Using next-generation sequencing analysis, we compared the VS and endometrial fluid (EF) microbiota in infertile women with (n = 20) or without CE (n = 103). Results: The detection rate of Streptococcus and Enterococcus as well as the bacterial abundance of Atopobium and Bifidobacterium in the VS microbiota was significantly lower in the CE group than in the non-CE group. Meanwhile, the detection rate and bacterial abundance of Lactobacillus in the EF and VS microbiota were at similar levels between the two groups. Conclusion: These findings suggest that VS microbiota in infertile women with CE is characterized by the reduction in Bifidobacterium and lactic-acid-producing bacteria other than Lactobacillus. Our results hold promise for the prediction of CE, not by somewhat interventional intrauterine procedures, but by less invasive VS sampling. TRIAL REGISTRATION NUMBER: UMIN000029449 (registration date 6 October 2017).
Practical Nonlinear Model Predictive Controller Design for Trajectory Tracking of Unmanned Vehicles
Authors: Pang Hui , Liu Minhao , Hu Chuan , Liu Nan
Published: April 2022

The trajectory tracking issue of unmanned vehicles has attracted much attention recently, with the rapid development and implementation of sensing, communication, and computing technologies. This paper proposes a nonlinear model predictive controller (NMPC) for the trajectory tracking application of an unmanned vehicle (UV). First, a two-degree-of-freedom (2-DOF) kinematics model of this UV is used to derive the desirable controller with two control variables as forward velocity and yaw angle. Next, the one-step Euler method is employed to establish the nonlinear prediction model, then a nonlinear optimization objective function is formulated to minimize the tracking errors of forward velocity and yaw angle from a preset time-varying reference road. Finally, the effectiveness of the proposed NMPC scheme is assessed under two different driving scenarios via MATLAB simulations. The simulation results confirm that the proposed NMPC scheme reveals better control accuracy and computational efficiency than the standard MPC controller under two different prescribed roads. Moreover, an outdoor field test is conducted to verify the performance of the proposed NMPC scheme, and the results show that the proposed NMPC can be applied to the real vehicle and can improve the tracking accuracy and the driving stability of trajectory tracking.
Mobile Assisted Language Learning: Evaluation of Accessibility, Adoption, and Perceived Outcome among Students of Higher Education
Authors: Habib Sufyan , Haider Ahsanuddin , Suleman Samar Sufyan Mohammad , Akmal Syed , Khan Mohammed Arshad
Published: April 2022

The present study was aimed at analyzing students’ perceptions toward mobile assisted language learning (MALL), their use patterns, and their usefulness in their academic outcomes. The results indicated that students use widely different MALL software for different applications. In a survey of 581 students from Indian colleges and universities, the research identified five antecedent factors (namely, individual desire and motives, perceived ease of use, technological factors, social influence, and perceived usefulness) which influenced students in the areas of adopting MALL software, students’ readiness, students’ motivation, and the subsequent effect on student performances. The research further indicated that students’ readiness and their motivation level mediated the relationship between factors of adoption of MALL and perceived outcome. The relevance of positive language learning outcomes, theoretical contribution and managerial implications of the study are discussed.