bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of a Smartphone Application on the Reduction of Attentional Bias Toward Alcohol Among Students†
Authors: Flaudias Valentin , Zerhouni Oulmann , Chakroun-Baggioni Nadia , De Chazeron Ingrid , Llorca Pierre-Michel , Brousse Georges
Published: February 2022

Context

The recent development of “serious games” has produced encouraging results in maintaining adherence to health-related interventions. In alcohol research, several studies have shown that computerized training on attentional bias decreases alcohol consumption bias among students. However, these highly controlled experimental situations, do not allow for direct large-scale dissemination. Our objective is to evaluate an attentional bias remediation program using a gamified smartphone training procedure.

Methods

Fifty students from Clermont-Ferrand University were invited to participate in the study. After a cognitive assessment in the laboratory, the smartphone application was installed on each Student’s smartphone. Participants were randomly assigned to either the alcohol attentional training group or the control group Each student had to complete the 2-min program at least once a day for 15 days. After 15 days, a new cognitive assessment of attention bias was conducted in the laboratory. Forty-seven students were included in the study.

Results

Our analyses did not show any effect of the cognitive remediation program on attentional bias reduction between the two group [F(1, 44) < 1, p = 0.87], attentional performance [F(1, 45) = 1.63, p = 0.20] or inhibitory abilities [F(1, 45) < 1, p = 0.73]. These results were confirmed by Bayesian analyses.

Discussion

Despite the absence of group effects, both the alcohol and control (non-alcohol) version of this program appeared to reduce attentional bias and increase inhibition capacities in the subset of participants who had attentional bias for alcohol at baseline This pilot study identifies areas for improvement in smartphone applications for future developments. Attentional bias remediation programs remain an interesting way to explore.
Usage, Pleasure, Price, and Feeling: A Study on Shopping Orientation and Consumer Outcome
Authors: Zhao Shaoqiong , Chen Pu , Zhu Yan , Wei Feng , Liu Fangmei
Published: February 2022

Understanding the behavior of consumers and especially the purchase-related behavior has been a focus of research for the past decades. Thus, researchers and practitioners are curious to know how purchase patterns are different under different conditions such as product category, price, feeling, and so on. The primary focus of this study was to examine how the price of the products influences the purchase behavior of consumers across hedonic and utilitarian categories under regulatory focus theory (RFT). The secondary insight was to examine how mood can moderate this impact. We conducted three experimental studies to examine these research questions regarding the preference of consumers of hedonic (utilitarian) products when the price is low (high) and at different mood conditions in this purchase process. The results confirmed our hypothesis that product category has a significant impact on purchase choice of products and mood can mediate this impact. In the last section, we discussed the theoretical contribution, strategic insights for product designers and marketers, and possible future research directions.
Exploring the Use of Selection, Optimization, and Compensation Strategies Beyond the Individual Level in a Workplace Context – A Qualitative Case Study
Authors: Karlsen Iben Louise , Borg Vilhelm , Meng Annette
Published: February 2022

Due to aging populations and the prolonging of working lives, the number of senior workers will increase. Therefore, this study investigates the use of SOC strategies (Selection, Optimization, and Compensation) across organizational levels as a means for senior workers to maintain workability and age successfully at work. The need to expand the perspective of the SOC model beyond the individual level, when applied to a work context, has been emphasized theoretically in the literature, nevertheless, SOC strategies have so far only been examined at the individual level. This study is the first to explore SOC strategies at the organizational, leadership, and group level. We focus on senior employees and the SOC strategies they use to balance out demands and limited resources. Based on 23 semi-structured interviews with senior employees and immediate managers at two hospitals (nurses), and two dairies (skilled/unskilled workers), we explore which specific SOC strategies are used at each level and reflect on the applicability of broadening the perspective of the SOC model when applying it to a work context. Based on the empirical findings and the discussion of the empirical exploration of SOC strategies beyond the individual level, we argue that it is advantageous to further pursue this line of inquiry and include the group, leadership, and organizational level when applying the SOC model in a work setting.
Depression, COVID-19 Anxiety, Subjective Well-being, and Academic Performance in University Students With COVID-19-Infected Relatives: A Network Analysis
Authors: Ventura-León José , Caycho-Rodríguez Tomás , Talledo-Sánchez Karim , Casiano-Valdivieso Kenia
Published: February 2022

This study aimed to examine the relationship between anxiety, depression, subjective well-being, and academic performance in Peruvian university health science students with COVID-19-infected relatives. Eight hundred two university students aged 17–54 years (Mean 21.83; SD = 5.31); 658 females (82%) and 144 males (18%); who completed the Patient Health Questionnaire-2, Coronavirus Anxiety Scale, Subjective Well-being Scale (SWB), and Self-reporting of Academic Performance participated. A partial unregularized network was estimated using the ggmModSelect function. Expected influence (EI) values were calculated to identify the central nodes and a two-tailed permutation test for the difference between the two groups (COVID-19 infected and uninfected). The results reveal that a depression and well-being node (PHQ1-SWB3) presents the highest relationship. The most central nodes belonged to COVID-19 anxiety, and there are no global differences between the comparison networks; but at the local level, there are connections in the network of COVID-19-infected students that are not in the group that did not present this diagnosis. It is concluded that anxious–depressive symptomatology and its relationship with well-being and evaluation of academic performance should be considered in order to understand the impact that COVID-19 had on health sciences students.
Linking Ethical Leadership to Followers’ Knowledge Sharing: Mediating Role of Psychological Ownership and Moderating Role of Professional Commitment
Authors: Saeed Imran , Khan Jawad , Zada Muhammad , Zada Shagufta , Vega-Muñoz Alejandro , Contreras-Barraza Nicolás
Published: February 2022

This study examined (1) the influence of ethical leadership on knowledge sharing, (2) the mediating role of psychological ownership, and (3) the moderating effect of professional commitment between ethical leadership (EL) and knowledge sharing (KS). Data were collected from 307 public listed Pakistani companies’ employees. Statistical analyses were performed by using SPSS Version 25 and AMOS version 22. The findings indicate a positive relationship between EL and KS behavior. Additionally, the impact of EL on KS was partially mediated by psychological ownership. Furthermore, professional commitment buffers the link between EL and KS. This study contributes to the body of knowledge in the field of leadership by confirming the role of ethics. The results show that ethical leaders develop employee attitudes (i.e., psychological ownership and professional commitment) that contribute to employee KS behavior. Ethical leaders create and encourage a learning culture to enhance organizational performance. This study adds to the little data on the positive impact of EL on listed company’s employees and addresses the gaps in previous studies on the role of EL in changing environments. In addition, professional commitment as a moderator has not been previously investigated with ethical leadership antecedents.
Treatment of Pregnant Women With Fear of Childbirth Using EMDR Therapy: Results of a Multi-Center Randomized Controlled Trial
Authors: Baas M. A. M. , van Pampus M. G. , Stramrood C. A. I. , Dijksman L. M. , Vanhommerig J. W. , de Jongh A.
Published: February 2022

Fear of childbirth (FoC) occurs in 7. 5% of pregnant women and has been associated with adverse feto-maternal outcomes. Eye Movement Desensitization and Reprocessing (EMDR) therapy has proven to be effective in the treatment of posttraumatic stress disorder (PTSD) and anxiety; however, its effectiveness regarding FoC has not yet been established. The aim was to determine the safety and effectiveness of EMDR therapy for pregnant women with FoC. This single-blind RCT (the OptiMUM-study, www.trialregister.nl, NTR5122) was conducted in the Netherlands. FoC was defined as a score ≥85 on the Wijma Delivery Expectations Questionnaire (WDEQ-A). Pregnant women with FoC and a gestational age between 8 and 20 weeks were randomly assigned to EMDR therapy or care-as-usual (CAU). The severity of FoC was assessed using the WDEQ-A. Safety was indexed as worsening of FoC symptoms, dropout, serious adverse events, or increased suicide risk. We used linear mixed model analyses to compare groups. A total of 141 women were randomized (EMDR n = 70; CAU n = 71). No differences between groups were found regarding safety. Both groups showed a very large (EMDR d = 1.36) or large (CAU d = 0.89) reduction of FoC symptoms with a mean decrease of 25.6 (EMDR) and 17.4 (CAU) points in WDEQ-A sum score. No significant difference between both groups was found (p = 0.83). At posttreatment, 72.4% (EMDR) vs. 59.6% (CAU) no longer met the criteria for FoC. In conclusion, the results are supportive of EMDR therapy as a safe and effective treatment of FoC during pregnancy, albeit without significant beneficial effects of EMDR therapy over and above those of CAU. Therefore, the current study results do not justify implementation of EMDR therapy as an additional treatment in this particular setting.
Perceived Effectiveness and Sustainability of Face Masks Among German Citizens During the 2nd Wave of the COVID-19 Pandemic—A Cross-Sectional Study
Authors: Fendt Maren Theresa Christin , Leal Filho Walter , Barbir Jelena , Boenecke Juliane
Published: February 2022

Background

The COVID-19 pandemic, which began at the end of 2019, has led to a significant increase in the demand for face masks in Germany and around the globe. Since non-reusable masks are often not correctly disposed of and are not biodegradable, their increasing use harms the environment. Both the ongoing pandemic and the rising environmental pollution eventually pose a threat to human health. Yet, it is unclear whether mask users are conscious of this, and which factors influence their choice of face masks. This study investigates the user preferences, perceived effectiveness, and the sustainability of different mouth/nose protection (MNP) to lay the foundation for developing more sustainable and effective alternatives.

Methods

A national (Germany-wide) cross-sectional study with a sample of 1,036 participants was conducted. Descriptive data analysis was deployed to describe trends and socio-demographic differences among the respondents. Different socio-demographic groups among the respondents were compared regarding their infection risk perception, compliance toward the use of personal protective measures, their choice of MNP, and knowledge level of sustainability and effectiveness of various MNP using inferential statistics (Chi2 test/Whitney–Mann-U-test/Kruskal–Wallis-test).

Results

The results suggest that, in addition to protective effectiveness, the reusability of MNP is important to not just most respondents but especially to older participants. In contrast, the price, shape, and design were not as important. The knowledge level of the effectiveness and sustainability of MNP was high among the participants and was not associated with socioeconomic characteristics. However, the knowledge level directly influenced the choice of MNP.

Conclusion

There seems to be an inclination to use sustainable MNP, provided their level of protection is similar to medical masks or FFP2/FFP3 masks. The willingness to wear a sustainable option increases with age.
Predicting Individual Treatment Response to rTMS for Motor Recovery After Stroke: A Review and the CanStim Perspective
Authors: Hildesheim Franziska E. , Silver Alexander N. , Dominguez-Vargas Adan-Ulises , Andrushko Justin W. , Edwards Jodi D. , Dancause Numa , Thiel Alexander
Published: February 2022

Background

Rehabilitation is critical for reducing stroke-related disability and improving quality-of-life post-stroke. Repetitive transcranial magnetic stimulation (rTMS), a non-invasive neuromodulation technique used as stand-alone or adjunct treatment to physiotherapy, may be of benefit for motor recovery in subgroups of stroke patients. The Canadian Platform for Trials in Non-Invasive Brain Stimulation (CanStim) seeks to advance the use of these techniques to improve post-stroke recovery through clinical trials and pre-clinical studies using standardized research protocols. Here, we review existing clinical trials for demographic, clinical, and neurobiological factors which may predict treatment response to identify knowledge gaps which need to be addressed before implementing these parameters for patient stratification in clinical trial protocols.

Objective

To provide a review of clinical rTMS trials of stroke recovery identifying factors associated with rTMS response in stroke patients with motor deficits and develop research perspectives for pre-clinical and clinical studies.

Methods

A literature search was performed in PubMed, using the Boolean search terms stroke AND repetitive transcranial magnetic stimulation OR rTMS AND motor for studies investigating the use of rTMS for motor recovery in stroke patients at any recovery phase. A total of 1,676 articles were screened by two blinded raters, with 26 papers identified for inclusion in this review.

Results

Multiple possible factors associated with rTMS response were identified, including stroke location, cortical thickness, brain-derived neurotrophic factor (BDNF) genotype, initial stroke severity, and several imaging and clinical factors associated with a relatively preserved functional motor network of the ipsilesional hemisphere. Age, sex, and time post-stroke were generally not related to rTMS response. Factors associated with greater response were identified in studies of both excitatory ipsilesional and inhibitory contralesional rTMS. Heterogeneous study designs and contradictory data exemplify the need for greater protocol standardization and high-quality controlled trials.

Conclusion

Clinical, brain structural and neurobiological factors have been identified as potential predictors for rTMS response in stroke patients with motor impairment. These factors can inform the design of future clinical trials, before being considered for optimization of individual rehabilitation therapy for stroke patients. Pre-clinical models for stroke recovery, specifically developed in a clinical context, may accelerate this process.
Can Transcranial Electrical Stimulation Facilitate Post-stroke Cognitive Rehabilitation? A Systematic Review and Meta-Analysis
Authors: Khan Ahsan , Yuan Kai , Bao Shi-Chun , Ti Chun Hang Eden , Tariq Abdullah , Anjum Nimra , Tong Raymond Kai-Yu
Published: February 2022

Background

Non-invasive brain stimulation methods have been widely utilized in research settings to manipulate and understand the functioning of the human brain. In the last two decades, transcranial electrical stimulation (tES) has opened new doors for treating impairments caused by various neurological disorders. However, tES studies have shown inconsistent results in post-stroke cognitive rehabilitation, and there is no consensus on the effectiveness of tES devices in improving cognitive skills after the onset of stroke.

Objectives

We aim to systematically investigate the efficacy of tES in improving post-stroke global cognition, attention, working memory, executive functions, visual neglect, and verbal fluency. Furthermore, we aim to provide a pathway to an effective use of stimulation paradigms in future studies.

Methods

Preferred reporting items for systematic reviews and meta-analysis (PRISMA) guidelines were followed. Randomized controlled trials (RCTs) were systematically searched in four different databases, including Medline, Embase, Pubmed, and PsychInfo. Studies utilizing any tES methods published in English were considered for inclusion. Standardized mean difference (SMD) for each cognitive domain was used as the primary outcome measure.

Results

The meta-analysis includes 19 studies assessing at least one of the six cognitive domains. Five RCTs studying global cognition, three assessing visual neglect, five evaluating working memory, three assessing attention, and nine studies focusing on aphasia were included for meta-analysis. As informed by the quantitative analysis of the included studies, the results favor the efficacy of tES in acute improvement in aphasic deficits (SMD = 0.34, CI = 0.02–0.67, p = 0.04) and attention deficits (SMD = 0.59, CI = −0.05–1.22, p = 0.07), however, no improvement was observed in any other cognitive domains.

Conclusion

The results favor the efficacy of tES in an improvement in aphasia and attentive deficits in stroke patients in acute, subacute, and chronic stages. However, the outcome of tES cannot be generalized across cognitive domains. The difference in the stimulation montages and parameters, diverse cognitive batteries, and variable number of training sessions may have contributed to the inconsistency in the outcome. We suggest that in future studies, experimental designs should be further refined, and standardized stimulation protocols should be utilized to better understand the therapeutic effect of stimulation.
Gas-phase Chemistry in the Interstellar Medium: The Role of Laboratory Astrochemistry
Authors: Puzzarini Cristina
Published: February 2022

“Who” and how? In this simple question the complexity of the interstellar chemistry is encapsulated. “Who” refers to what molecules are present in the interstellar medium (ISM) and “how” to the mechanisms that led to their formation. While the large number of molecules discovered in the ISM (∼250) demonstrates the rich chemistry occurring there, a significant number of unknown species are waiting for an identification and the processes that led to the synthesis of the identified species are still hotly debated or even unknown. Gas-phase laboratory studies in the fields of rotational spectroscopy and quantum chemistry provide an important contribution to answering the question above. An overview on the role played by rotational spectroscopy and quantum chemistry in the unraveling of the gas-phase chemistry of the interstellar medium is presented.
Comparison of the Axes and Positions of the Uterus and Vagina Between Women With and Without Pelvic Floor Organ Prolapse
Authors: Li Song , Wen Xuewei , Gao Zhenhua , Ke Kunbin , Yang Jing , Wang Haifeng , Mo Yin , Zeng Yizhen , Li Yuan , Tian Daoming , Shen Jihong
Published: February 2022

Purpose

To analyze the role of the axial positions of the uterus and vagina in providing pelvic floor support, encourage evaluations of pelvic floor function, and improve the understanding of the pathogenesis of pelvic organ prolapse.

Methods

The lengths and angles of the upper, middle, and lower axes of the vagina, uterine body, and cervix of 81 women with prolapse (prolapse group) and 57 women without prolapse (non-prolapse group) were measured and compared using magnetic resonance images. The pelvic inclination correction system (PICS) line was also compared between the groups. The coordinate parameters of the anatomical points of the uterus and vagina were measured, and their positions were analyzed.

Results

In the prolapse group, the uterine body-cervical angle, cervical-upper vaginal angle, uterine body-PICS line angle, cervical-PICS line angle, and lower vaginal-PICS line angle were smaller (p < 0.05) and the middle-lower vaginal angle, upper vaginal-PICS line angle, and middle vaginal-PICS line angles were larger (p < 0.05) than those in the non-prolapse group. The cervical length was longer (p < 0.05) and the middle and lower vaginal lengths were shorter (p < 0.05) in the prolapse group. The coordinate system revealed that the uterine and vaginal axes were shifted backward and downward in the prolapse group.

Conclusion

Patients in the prolapse group were more likely to have retroversion and retroflexion of the uterus than those in the non-prolapse group. The vagina was shortened, turned forward, and straightened, and the uterus and vagina were shifted backward and downward in the prolapse group. Changes in the axial position of the uterus and vagina are important mechanisms of pelvic floor organ prolapse.
Evaluating the Impact of Neurosurgical Rotation Experience in Africa on the Interest and Perception of Medical Students Towards a Career in Neurosurgery: A Continental, Multi-Centre, Cross-Sectional Study
Authors: Dada Olaoluwa Ezekiel , Ooi Setthasorn Zhi Yang , Bukenya George William , Kenfack Yves Jordan , Le Chi , Ohonba Efosa , Adeyemo Emmanuel , Narain Kapil , Awad Ahmed K. , Barrie Umaru , Sichimba Dawin , Ogunfolaji Oloruntoba , Kitonga Lilian Mwende , Oriaku Adaeze Juanita , Bamimore Michael A. , Okor Douglas Emeka , Rominiyi Ola
Published: February 2022

Objective

Africa has the second highest neurosurgical workforce deficit globally and many medical students in Africa lack exposure to the field. This study aims to assess the impact of a neurosurgical rotation during medical school in shaping the perception and interest of students toward a career in neurosurgery.

Study Design

Cross-sectional study.

Methods

A Google form e-survey was disseminated to African clinical medical students between February 21st and March 20th, 2021. Data on exposure and length of neurosurgical rotation and perception of, and interest in, neurosurgery were collected. Data was analyzed using descriptive statistics and adjusted logistic regression modeling.

Results

Data was received from 539 students in 30 African countries (30/54, 55.6%). The majority of participants were male and were from Kenya, Nigeria and South Africa. Most students had undertaken a formal neurosurgery rotation, of which the majority reported a rotation length of 4 weeks or less. Students who had more than 4 weeks of neurosurgical exposure were more likely to express a career interest in neurosurgery than those without [odds ratio (OR) = 1.75, p < 0.04] and men were more likely to express interest in a neurosurgical career compared to women (OR = 3.22, p < 0.001), after adjusting for other factors.

Conclusion

Neurosurgical exposure is a key determinant in shaping the perception and interest of medical students toward a career in neurosurgery. Our findings support the need: i) for a continent-wide, standardized curriculum guide to neurosurgical rotations and ii) to advocate for gender inclusivity in education and policy-making efforts across the African continent.
Beyond Numbers: Determining the Socioeconomic and Livelihood Impacts of African Swine Fever and Its Control in the Philippines
Authors: Cooper Tarni L. , Smith Dominic , Gonzales Mark Jaypee C. , Maghanay Marlon T. , Sanderson Sunny , Cornejo Marie Rachelle Jane C. , Pineda Lohreihleih L. , Sagun Rose Ann A. , Salvacion Oliver P.
Published: February 2022

The impacts of African Swine Fever (ASF) have most frequently been described quantitatively though it is increasingly acknowledged these impacts extend well beyond numbers. During 2020, a multidisciplinary team of researchers developed a framework for Socioeconomic and Livelihood Impact Assessment (SELIA) of livestock diseases in smallholder communities. Two key innovations within this SELIA framework are the integration of sustainable livelihoods concepts to capture rich information beyond financial impacts, and the inclusion of stakeholders across the value chain, beyond farmers. This paper focuses on the findings from one of the first applications of the SELIA framework. In late 2020 the research team applied participatory tools from the SELIA Framework (8 focus group discussions, 14 key informant interviews, and 2 network mapping activities) to gather data to describe the impact of ASF in backyard pig-farming communities and value chains. This was undertaken across two locations in the Philippines, in turn highlighting potential leverage points for intervention. Owing to COVID-19 travel restrictions and risks, modifications to training and field activities were made. Findings from focus groups and interviews revealed the deep, emotional impacts of ASF and the associated control measures. Pigs were considered pets by many farmers and some women described them as being like their children. Animal health-workers (AHWs) also recognised the emotional toll on farmers and were sometimes strongly criticised by community members due to their involvement in depopulation campaigns. Misinformation early in the epidemic also led farmers to hide their animals from AHWs, and to dispose of them inappropriately. While the overall impact of ASF on society was negative, the impacts across different communities, scales of production and different value chain actors varied. The losses experienced by backyard farmers resulted in significant losses to linked value chain actors, such as input suppliers. This trial application of the SELIA framework revealed some complex and varied impacts of ASF. This included significant differences in livelihood and socio-economic impacts amongst different actors within value chains and also among different categories of actors (for example small, medium and large-scale traders). Repeated themes and triangulated findings suggest two leverage points for further consideration. Firstly, it is recommended a One Welfare approach to ASF control in the Philippines is explored. Emphasising careful communication between animal health-workers and farmers, and humane and sensitive pig depopulation practices. Secondly, consideration of ASF support programs tailored to sectoral and specific communities is recommended.
Assessment of the Family Context in Adolescence: A Systematic Review
Authors: Oropesa Ruiz Nieves Fátima
Published: February 2022

Background: Bearing in mind that the characteristics of the family system have a significant influence on the positive development of adolescents and considering that there are different measuring instruments, the main objective of this work is to review studies on the instruments for evaluating the family context, to determine which instruments are validated or adapted by researchers between 2010 and 2020 and which variables in the family context are valued during the adolescent stage. Methods: The academic search engines consulted have been Scopus, Redalyc and Web of Science. Following the criteria contemplated in the PRISMA Declaration, once duplicates were eliminated, a total of 101 studies were identified. A critical reading of the titles, summaries and a large part of the complete articles was carried out, and 56 studies were excluded. Finally, a systematic review of 45 studies that contrasted the psychometric properties of self-report measures (questionnaires, inventories, scales), between original papers and adaptations was carried out. Results: The results obtained reveal that the instruments measure different aspects of the family system: the family dynamics (variables such as parental competence, resilience, social support, parenting style and practices of leisure and free time); family functioning (variables such as problem solving, communication, roles, affective response capacity, affective participation, behavior control, cohesion, adaptability and family satisfaction); family adjustment (variables referring to parental stress, parental conflict, family health and family protection; and the parent–child relationships (variables such as quality, family effectiveness, family atmosphere and attachment). Conclusions: The most used psychological tests are: Parental Bonding Instrument, Family Assessment Resources, Social Support Scale, Parental Stress Index and Scale of Adaptation, Participation, Gain, Affection and Resources. Parental Bonding Instrument shows excellent psychometric properties. The rest of the self-report measures present acceptable reliability indices. The psychometric properties of some Family Assessment Resources, Social Systems Assessment Scale and Protective Factors Survey scales are more questionable, so new validation studies of these instruments are required. Affect (quality of relationships, manifestation, attachment bond), communication between members of the family group and parental control (behavioral and psychological) have been the main dimensions of the family context studied in adolescence. Along with these variables, others have been incorporated, such as parental resilience, family leisure and free time routines, family health or family strengths and weaknesses. The related instruments used in different psychological and cultural environments may help us to better understand the educational and parenting practices based on family dynamics, functioning, adjustment and parent–child relationships.
The Synergetic Effect of Soil Amendments on Reducing Bioavailable Heavy Metals and Greenhouse Gas Emissions from Upland Soil
Authors: Hong Young-Kyu , Kim Jin-Wook , Kim Hyuck-Soo , Yang Jae-E. , Kim Sung-Chul
Published: February 2022

Heavy metal pollution and greenhouse gas (GHG) emissions from soil are two major detrimental sources in the agriculture environment because of concerns about crop safety and global warming. Applying amendments on site is a common technique used for heavy metal remediation and the reduction in GHG emissions. This study aims to evaluate the effect of different amendments on the reduction in both bioavailable heavy metals and GHG emissions from soil. Four different amendments, namely bottom ash (BA), sintered material (SM), sintered material combined with lime (SM + L), and FeO (SM + FeO) were applied to upland fields, followed by maize (Zea mays L.) cultivation from April to October. Subsequently, SM + FeO treatment demonstrated the highest bioavailability reduction efficiency for As at 79.1%, and SM + L treatment had a high efficiency for the reduction in Cd and Pb by 64.6% and 41.4%, respectively. SM + FeO treatment also reduced bioaccumulated As and Pb in maize grain by 59.3% and 66.2%, respectively. Furthermore, SM + FeO treatment demonstrated the highest reduction efficiency for cumulative N2O emissions by 70.7%, compared to the control soil. Among the four different amendments, the efficiency of heavy metal and GHG emission reduction was in the following order: SM + FeO > SM + L > SM > BA. Overall, SM combined with FeO is a promising amendment for reducing and managing both heavy metal pollution and GHG emissions in soil.
Detecting Crown Rot Disease in Wheat in Controlled Environment Conditions Using Digital Color Imaging and Machine Learning
Authors: Xie Yiting , Plett Darren , Liu Huajian
Published: February 2022

Crown rot is one of the major stubble soil fungal diseases that bring significant yield loss to the cereal industry. The most effective crown rot management approach is removal of infected crop residue from fields and rotation of nonhost crops. However, disease screening is challenging as there are no clear visible symptoms on upper stems and leaves at early growth stages. The current manual screening method requires experts to observe the crown and roots of plants to detect disease, which is time-consuming, subjective, labor-intensive, and costly. As digital color imaging has the advantages of low cost and easy use, it has a high potential to be an economical solution for crown rot detection. In this research, a crown rot disease detection method was developed using a smartphone camera and machine learning technologies. Four common wheat varieties were grown in greenhouse conditions with a controlled environment, and all infected group plants were infected with crown rot without the presence of other plant diseases. We used a smartphone to take digital color images of the lower stems of plants. Using imaging processing techniques and a support vector machine algorithm, we successfully distinguished infected and healthy plants as early as 14 days after disease infection. The results provide a vital first step toward developing a digital color imaging phenotyping platform for crown rot detection to enable the management of crown rot disease effectively. As an easy-access phenotyping method, this method could provide support for researchers to develop an efficiency and economic disease screening method in field conditions.
Pharmacists’ Perspectives of Their Roles in Antimicrobial Stewardship: A Qualitative Study among Hospital Pharmacists in Malaysia
Authors: Lai Wan Mae , Islahudin Farida Hanim , Ambaras Khan Rahela , Chong Wei Wen
Published: February 2022

Antimicrobial resistance has negatively impacted patient outcomes and increased healthcare costs. Antimicrobial stewardship (AMS) includes all activities and policies to promote the judicious use of antimicrobials. Pharmacists are key players in AMS models worldwide. However, there is a research gap in the role of pharmacists as antimicrobial stewards in Malaysia. This study aimed to explore hospital pharmacists’ perspectives on their roles in, and barriers and facilitators to the implementation of AMS strategies. Individual, semi-structured interviews were conducted with 16 hospital pharmacists involved in AMS activities from 13 public hospitals in Kuala Lumpur and Selangor. Audio-taped interviews were transcribed verbatim and imported into NVivo software version 10.0 (QSR). A thematic analysis method was used to identify themes from the qualitative data until theme saturation was reached. Respondents perceived pharmacists as having important roles in the implementation of AMS strategies, in view of the multiple tasks they were entrusted with. They described their functions as antimicrobial advisors, antimicrobial guardians and liaison personnel. The lack of resources in terms of training, manpower and facilities, as well as attitudinal challenges, were some barriers identified by the respondents. Administrative support, commitment and perseverance were found to be facilitators to the role of pharmacists in AMS. In conclusion, pharmacists in public hospitals play important roles in AMS teams. This study has provided insights into the support that AMS pharmacists in public hospitals require to overcome the barriers they face and to enhance their roles in the implementation of AMS strategies.
Associations between Oxidative/Nitrosative Stress and Thyroid Hormones in Pregnant Women—Tainan Birth Cohort Study (TBCS)
Authors: Cheng Po-Keng , Chen Hsin-Chang , Kuo Pao-Lin , Chang Jung-Wei , Chang Wan-Ting , Huang Po-Chin
Published: February 2022

Oxidative and nitrosative stress have been linked to thyroid function in both animal and human studies. In the present study, the associations between oxidative and nitrosative stress and thyroid hormones were investigated. Measurements were obtained from 97 Taiwanese pregnant women at the first, second, and third trimesters. Levels of five oxidative and nitrosative stress biomarkers (8-hydroxy-2′-deoxyguanosine [8-OHdG], 8-nitroguanine [8-NO2Gua], 4-hydroxy-2-nonenal-mercapturic acid [HNE-MA], 8-isoprostaglandin F2α [8-isoPGF2α], and malondialdehyde [MDA]) were measured using urine samples, and levels of five thyroid hormones (triiodothyronine [T3], thyroxine [T4], free T4, thyroid-stimulating hormone [TSH], and T4-binding globulin [TBG]) were measured in blood samples. Multiple linear regressions and linear mixed-model regressions were conducted to determine the associations between oxidative or nitrosative stress biomarkers and thyroid hormones in pregnant women. We found that TSH was negatively and significantly associated with 8-NO2Gua (−14%, 95% CI [−26.9% to −1.1%]) and HNE-MA (−23%, 95% CI [−35.9% to −10.0%]) levels. However, T4 (3%, 95% CI [0.2%–5.8%]) and free T4 (4.3%, 95% CI [0.8%–7.8%]) levels were positively and significantly associated with 8-NO2Gua. The T4 to TBG and free T4 to TBG ratios were positively and significantly associated with 8-NO2Gua level (T4/TBG: 3.6%, 95% CI [0.5%–6.7%]; free T4/TBG: 5.6%, 95% CI [0.2%–11.1%]). However, the TSH to T4 ratio was negatively and significantly associated with 8-NO2Gua level (−17.3%, 95% CI [−30.4% to −4.3%]). The T3 to TSH ratio was positively and significantly associated with HNE-MA level (25.2%, 95% CI [11.2%–39.2%]). However, the TSH to T4 and TSH to free T4 ratios were negatively and significantly associated with HNE-MA level (TSH/T4: −21.2%, 95% CI [−34.5% to −7.8%] and TSH/free T4: −24.0%, 95% CI [−38.3% to −9.6%]). Our findings suggest that an imbalance of oxidative and nitrosative stress may alter thyroid hormone homeostasis during pregnancy. Disruption of the maternal thyroid homeostasis during pregnancy would affect embryonic and fetal development.
Effect of Magnesium Ion on the Radical-Scavenging Rate of Pterostilbene in an Aprotic Medium: Mechanistic Insight into the Antioxidative Reaction of Pterostilbene
Authors: Nakanishi Ikuo , Shoji Yoshimi , Ohkubo Kei , Ueno Megumi , Shimoda Kei , Matsumoto Ken-ichiro , Fukuhara Kiyoshi , Hamada Hiroki
Published: February 2022

Pterostilbene (PTS), a methylated analog of resveratrol (RSV), has recently attracted much attention due to its enhanced bioavailability compared to RSV. However, little is known about the radical-scavenging mechanism of PTS. In this study, we investigated the effect of Mg(ClO4)2 on the scavenging reaction of galvinoxyl radical (GO•) by PTS in acetonitrile (MeCN). GO• was used as a model for reactive oxygen radicals. The second-order rate constant (kH) for the GO•-scavenging reaction by PTS was more than threefold larger than that by RSV, although thermodynamic parameters, such as the relative O–H bond dissociation energies of the phenolic OH groups, ionization potentials, and HOMO energies calculated by the density functional theory are about the same between PTS and RSV. The oxidation peak potential of PTS determined by the cyclic voltammetry in MeCN (0.10 M Bu4NClO4) was also virtually the same as that of RSV. On the other hand, no effect of Mg (ClO4)2 on the kH values was observed for PTS, in contrast to the case for RSV. A kinetic isotope effect of 3.4 was observed when PTS was replaced by a deuterated PTS. These results suggest that a one-step hydrogen-atom transfer from PTS to GO• may be the rate-determining step in MeCN.
Pre-Exposure to Stress-Inducing Agents Increase the Anticancer Efficacy of Focused Ultrasound against Aggressive Prostate Cancer Cells
Authors: Murad Hakm Y. , Chandra Partha K. , Kelly Charles A. , Khurana Namrata , Yu Heng , Bortz Emma P. , Hong Shirley N. , Mondal Debasis , Khismatullin Damir B.
Published: February 2022

Despite the initial success in treatment of localized prostate cancer (PCa) using surgery, radiation or hormonal therapy, recurrence of aggressive tumors dictates morbidity and mortality. Focused ultrasound (FUS) is being tested as a targeted, noninvasive approach to eliminate the localized PCa foci, and strategies to enhance the anticancer potential of FUS have a high translational value. Since aggressive cancer cells utilize oxidative stress (Ox-stress) and endoplasmic reticulum stress (ER-stress) pathways for their survival and recurrence, we hypothesized that pre-treatment with drugs that disrupt stress-signaling pathways in tumor cells may increase FUS efficacy. Using four different PCa cell lines, i.e., LNCaP, C4-2B, 22Rv1 and DU145, we tested the in vitro effects of FUS, alone and in combination with two clinically tested drugs that increase Ox-stress (i.e., CDDO-me) or ER-stress (i.e., nelfinavir). As compared to standalone FUS, significant (p < 0.05) suppressions in both survival and recurrence of PCa cells were observed following pre-sensitization with low-dose CDDO-me (100 nM) and/or nelfinavir (2 µM). In drug pre-sensitized cells, significant anticancer effects were evident at a FUS intensity of as low as 0.7 kW/cm2. This combined mechanochemical disruption (MCD) approach decreased cell proliferation, migration and clonogenic ability and increased apoptosis/necrosis and reactive oxygen species (ROS) production. Furthermore, although activated in cells that survived standalone FUS, pre-sensitization with CDDO-me and/or nelfinavir suppressed both total and activated (phosphorylated) NF-κB and Akt protein levels. Thus, a combined MCD therapy may be a safe and effective approach towards the targeted elimination of aggressive PCa cells.
Active Correction for Incremental Speaker Diarization of a Collection with Human in the Loop
Authors: Prokopalo Yevhenii , Shamsi Meysam , Barrault Loïc , Meignier Sylvain , Larcher Anthony
Published: February 2022

State of the art diarization systems now achieve decent performance but those performances are often not good enough to deploy them without any human supervision. Additionally, most approaches focus on single audio files while many use cases involving multiple recordings with recurrent speakers require the incremental processing of a collection. In this paper, we propose a framework that solicits a human in the loop to correct the clustering by answering simple questions. After defining the nature of the questions for both single file and collection of files, we propose two algorithms to list those questions and associated stopping criteria that are necessary to limit the work load on the human in the loop. Experiments performed on the ALLIES dataset show that a limited interaction with a human expert can lead to considerable improvement of up to 36.5% relative diarization error rate (DER) for single files and 33.29% for a collection.
Correlations and Asymptotic Behaviors of the Shape Parameters of Floating Bubbles Using an Improved Numerical Procedure
Authors: Yeom Geum-Su
Published: February 2022

An improved numerical procedure is used to present the correlations between the shape parameters and Bond numbers of floating bubbles for a wider range of Bond numbers (5×10−5<Bo<5000) than the previously reported range of Bond numbers (0.003<Bo<241), and their asymptotic relations as Bo → 0 and Bo → ∞. The proposed method is proven to be more precise and robust than the conventional methods in comparison with previous numerical and experimental results. In addition, the profile of floating bubbles and the related parameters are presented for a wide range of bubble sizes. The shape parameters are divided into three distinct Bond number regions, and are fitted with a fifth-order polynomial as a function of Bond number on a log-log scale for each region. The parameters show two asymptotes, which can be expressed in a simple power law. In addition, the dimensionless maximum depth of the floating bubble is obtained as H=0.7291015 when Bo=4.755563. These correlations and asymptotic relations are expected to assist in the development of scale models of dynamic bubble-related phenomena such as bubble bursting.
Vasorelaxant Activity of AP39, a Mitochondria-Targeted H2S Donor, on Mouse Mesenteric Artery Rings In Vitro
Authors: da Costa Marques Leonardo A. da Costa , Teixeira Simone A. , de Jesus Flávia N. de , Wood Mark E. , Torregrossa Roberta , Whiteman Matthew , Costa Soraia K. P. , Muscará Marcelo N.
Published: February 2022

Mitochondria-targeted hydrogen sulfide (H2S) donor compounds, such as compound AP39, supply H2S into the mitochondrial environment and have shown several beneficial in vitro and in vivo effects in cardiovascular conditions such as diabetes and hypertension. However, the study of their direct vascular effects has not been addressed to date. Thus, the objective of the present study was to analyze the effects and describe the mechanisms of action of AP39 on the in vitro vascular reactivity of mouse mesenteric artery. Protein and gene expressions of the H2S-producing enzymes (CBS, CSE, and 3MPST) were respectively analyzed by Western blot and qualitative RT-PCR, as well the in vitro production of H2S by mesenteric artery homogenates. Gene expression of CSE and 3MPST in the vessels has been evidenced by RT-PCR experiments, whereas the protein expression of all the three enzymes was demonstrated by Western blotting experiments. Nonselective inhibition of H2S-producing enzymes by AOAA abolished H2S production, whereas it was partially inhibited by PAG (a CSE selective inhibitor). Vasorelaxation promoted by AP39 and its H2S-releasing moiety (ADT-OH) were significantly reduced after endothelium removal, specifically dependent on NO-cGMP signaling and SKCa channel opening. Endogenous H2S seems to participate in the mechanism of action of AP39, and glibenclamide-induced KATP blockade did not affect the vasorelaxant response. Considering the results of the present study and the previously demonstrated antioxidant and bioenergetic effects of AP39, we conclude that mitochondria-targeted H2S donors may offer a new promising perspective in cardiovascular disease therapeutics.
Mcm5 Represses Endodermal Migration through Cxcr4a-itgb1b Cascade Instead of Cell Cycle Control
Authors: Zhang Yu , Xia Jiamin , Liu Min , Chen Bingyu , Yang Min , Yu Xiaoping , Ou Yu , Li Shurong , Liu Xindong , Feng Yi , Su Bingyin , Huang Sizhou
Published: February 2022

Minichromosome maintenance protein 5 (MCM5) is a critical cell cycle regulator; its role in DNA replication is well known, but whether it is involved in the regulation of organogenesis in a cell cycle-independent way, is far from clear. In this study, we found that a loss of mcm5 function resulted in a mildly smaller liver, but that mcm5 overexpression led to liver bifida. Further, the data showed that mcm5 overexpression delayed endodermal migration in the ventral–dorsal axis and induced the liver bifida. Cell cycle analysis showed that a loss of mcm5 function, but not overexpression, resulted in cell cycle delay and increased cell apoptosis during gastrulation, implying that liver bifida was not the result of a cell cycle defect. In terms of its mechanism, our data proves that mcm5 represses the expression of cxcr4a, which sequentially causes a decrease in the expression of itgb1b during gastrulation. The downregulation of the cxcr4a-itgb1b cascade leads to an endodermal migration delay during gastrulation, as well as to the subsequent liver bifida during liver morphogenesis. In conclusion, our results suggest that in a cell cycle-independent way, mcm5 works as a gene expression regulator, either partially and directly, or indirectly repressing the expression of cxcr4a and the downstream gene itgb1b, to coordinate endodermal migration during gastrulation and liver location during liver organogenesis.