bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-term-video monitoring EEG and 18F-FDG-PET are useful tools to detect residual disease activity in anti-LGI1-Abs encephalitis: A case report
Authors: Cornacchini Sara , Farina Antonio , Contento Margherita , Berti Valentina , Biggi Martina , Barilaro Alessandro , Massacesi Luca , Damato Valentina , Rosati Eleonora
Published: August 2022

Background

The use of CD20-depleting monoclonal antibodies has shown to improve the long-term outcome of patients with anti-leucine-rich glioma-inactivated protein 1 antibodies (anti-LGI1-Abs) encephalitis after first-line immunotherapy, but currently predictive markers of treatment response and disease activity are lacking.

Case presentation

A 75-year-old man presented cognitive impairment and faciobrachial dystonic seizures (FBDS), with mild abnormalities at electroencephalography (EEG), normal brain magnetic resonance and cerebrospinal fluid (CSF) analysis. Anti-LGI1-Abs were detected in serum and CSF, and corticosteroids and intravenous immunoglobulins were administered. Despite partial cognitive improvement, 18F-fluoridesoxyglucose-positron emission tomography (18F-FDG-PET) showed the persistence of temporo-mesial hypermetabolism, and FBDS were still detected by long-term monitoring video EEG (LTMV EEG). Rituximab was therefore administered with FBDS disappearance, further cognitive improvement, and resolution of 18F-FDG-PET temporo-mesial hypermetabolism.

Conclusions

Our experience supports the use of 18F-FDG-PET and LTMVEEG as useful tools to measure disease activity, evaluate treatment response and guide therapeutic decisions in the long-term management of anti-LGI1-antibody encephalitis.
The effects of robot-assisted gait training combined with non-invasive brain stimulation on lower limb function in patients with stroke and spinal cord injury: A systematic review and meta-analysis
Authors: Kuwahara Wataru , Sasaki Shun , Yamamoto Rieko , Kawakami Michiyuki , Kaneko Fuminari
Published: August 2022

Objective:

This study aimed to investigate the effect of robot-assisted gait training (RAGT) therapy combined with non-invasive brain stimulation (NIBS) on lower limb function in patients with stroke and spinal cord injury (SCI).

Data sources

PubMed, Cochrane Central Register of Controlled Trials, Ovid MEDLINE, and Web of Science were searched.

Study selection

Randomized controlled trials (RCTs) published as of 3 March 2021. RCTs evaluating RAGT combined with NIBS, such as transcranial direct current stimulation (tDCS) and repetitive transcranial magnetic stimulation (rTMS), for lower limb function (e.g., Fugl-Meyer assessment for patients with stroke) and activities (i.e., gait velocity) in patients with stroke and SCI were included.

Data extraction

Two reviewers independently screened the records, extracted the data, and assessed the risk of bias.

Data synthesis

A meta-analysis of five studies (104 participants) and risk of bias were conducted. Pooled estimates demonstrated that RAGT combined with NIBS significantly improved lower limb function [standardized mean difference (SMD) = 0.52; 95% confidence interval (CI) = 0.06–0.99] but not lower limb activities (SMD = −0.13; 95% CI = −0.63–0.38). Subgroup analyses also failed to find a greater improvement in lower limb function of RAGT with tDCS compared to sham stimulation. No significant differences between participant characteristics or types of NIBS were observed.

Conclusion

This meta-analysis demonstrated that RAGT therapy in combination with NIBS was effective in patients with stroke and SCI. However, a greater improvement in lower limb function and activities were not observed using RAGT with tDCS compared to sham stimulation.
Head holder and cranial window design for sequential magnetic resonance imaging and optical imaging in awake mice
Authors: Mikkelsen Signe H. , Wied Boris , Dashkovskyi Vitalii , Lindhardt Thomas Beck , Hirschler Lydiane , Warnking Jan M. , Barbier Emmanuel L. , Postnov Dmitry , Hansen Brian , Gutiérrez-Jiménez Eugenio
Published: August 2022

Medical imaging techniques are widely used in preclinical research as diagnostic tools to detect physiological abnormalities and assess the progression of neurovascular disease in animal models. Despite the wealth of imaging options in magnetic resonance imaging (MRI), interpretation of imaging-derived parameters regarding underlying tissue properties is difficult due to technical limitations or lack of parameter specificity. To address the challenge of interpretation, we present an animal preparation protocol to achieve quantitative measures from both MRI and advanced optical techniques, including laser speckle contrast imaging and two-photon microscopy, in murine models. In this manner, non-translatable methods support and improve interpretation of less specific, translatable methods, i.e., MRI. Combining modalities for improved clinical interpretation involves satisfying the requirements of various methods. Furthermore, physiology unperturbed by anesthetics is a prerequisite for the strategy to succeed. Awake animal imaging with restraint provides an alternative to anesthesia and facilitates translatability of cerebral measurements. The method outlines design requirements for the setup and a corresponding reproducible surgical procedure for implanting a 3D printed head holder and cranial window to enable repeated multimodal imaging. We document the development, application, and validation of the method and provide examples confirming the usefulness of the design in acquiring high quality data from multiple modalities for quantification of a wide range of metrics of cerebral physiology in the same animal. The method contributes to preclinical small animal imaging, enabling sequential imaging of previously mutually exclusive techniques.
Phytonutrients: Sources, bioavailability, interaction with gut microbiota, and their impacts on human health
Authors: Kan Juntao , Wu Feng , Wang Feijie , Zheng Jianheng , Cheng Junrui , Li Yuan , Yang Yuexin , Du Jun
Published: August 2022

Phytonutrients are natural bioactive components present in the daily diet that can exert a positive impact on human health. Studies have shown that phytonutrients may act as antioxidants and improve metabolism after being ingested, which help to regulate physiological processes and prevent metabolic disorders and diseases. However, their efficacy is limited by their low bioavailability. The gut microbiota is symbiotic with humans and its abundance and profile are related to most diseases. Interestingly, studies have shown that the gut microbiota is associated with the metabolism of phytonutrients by converting them into small molecules that can be absorbed by the body, thereby enhancing their bioavailability. Furthermore, phytonutrients can modulate the composition of the gut microbiota, and therefore improve the host's health. Here, we focus on uncovering the mechanisms by which phytonutrients and gut microbiota play roles in health, and the interrelationships between phytonutrients and gut microbiota were summarized. We also reviewed the studies that reported the efficacy of phytonutrients in human health and the future directions.
Adhesion strength and anti-tumor agents regulate vinculin of breast cancer cells
Authors: Metsiou Despoina Nektaria , Deligianni Despina , Giannopoulou Efstathia , Kalofonos Haralabos , Koutras Angelos , Athanassiou George
Published: August 2022

The onset and progression of cancer are strongly associated with the dissipation of adhesion forces between cancer cells, thus facilitating their incessant attachment and detachment from the extracellular matrix (ECM) to move toward metastasis. During this process, cancer cells undergo mechanical stresses and respond to these stresses with membrane deformation while inducing protrusions to invade the surrounding tissues. Cellular response to mechanical forces is inherently related to the reorganization of the cytoskeleton, the dissipation of cell–cell junctions, and the adhesion to the surrounding ECM. Moreover, the role of focal adhesion proteins, and particularly the role of vinculin in cell attachment and detachment during migration, is critical, indicating the tight cell–ECM junctions, which favor or inhibit the metastatic cascade. The biomechanical analysis of these sequences of events may elucidate the tumor progression and the potential of cancer cells for migration and metastasis. In this work, we focused on the evaluation of the spreading rate and the estimation of the adhesion strength between breast cancer cells and ECM prior to and post-treatment with anti-tumor agents. Specifically, different tamoxifen concentrations were used for ER+ breast cancer cells, while even concentrations of trastuzumab and pertuzumab were used for HER2+ cells. Analysis of cell stiffness indicated an increased elastic Young’s modulus post-treatment in both MCF-7 and SKBR-3 cells. The results showed that the post-treatment spreading rate was significantly decreased in both types of breast cancer, suggesting a lower metastatic potential. Additionally, treated cells required greater adhesion forces to detach from the ECM, thus preventing detachment events of cancer cells from the ECM, and therefore, the probability of cell motility, migration, and metastasis was confined. Furthermore, post-detachment and post-treatment vinculin levels were increased, indicating tighter cell–ECM junctions, hence limiting the probability of cell detachment and, therefore, cell motility and migration.
Lentiviral vector–based xenograft tumors as candidate reference materials for detection of HER2-low breast cancer
Authors: Wei Yali , An Xu , Cao Qinmei , Che Nanying , Xue Yuanyuan , Deng Haiteng , Wang Qingtao , Zhou Rui
Published: August 2022

The human epidermal growth factor receptor 2 (HER2) is an important biomarker that plays a pivotal role in therapeutic decision-making for patients with breast cancer (BC). Patients with HER2-low BC can benefit from new HER2 targeted therapy. For ensuring the accurate and reproducible detection of HER2-low cancer, reliable reference materials are required for monitoring the sensitivity and specificity of detection assays. Herein, a lentiviral vector was used to transduce the HER2 gene into MDA-MB-231 cells that exhibited low HER2 density, and the cells were characterized by droplet digital PCR to accurately determine the copy number variation. Then, the formalin-fixed paraffin-embedded (FFPE) samples from xenografts were prepared and evaluated for suitability as candidate reference materials by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). The FFPE reference materials were selected on the basis of IHC score of 2+ and negative FISH result to meet the requirement for HER2-low BC detection. Furthermore, the FFPE reference materials exhibited typical histological structures that resembled the clinical BC specimens. These novel FFPE reference materials displayed the high stability and homogeneity, and they were produced in high quantity. In summary, we generated high-quality reference materials for internal quality control and proficiency testing in HER2-low detection.
Erythropoietin receptor regulates tumor mitochondrial biogenesis through iNOS and pAKT
Authors: Aboouf Mostafa A. , Guscetti Franco , von Büren Nadine , Armbruster Julia , Ademi Hyrije , Ruetten Maja , Meléndez-Rodríguez Florinda , Rülicke Thomas , Seymer Alexander , Jacobs Robert A. , Schneider Gasser Edith M. , Aragones Julian , Neumann Drorit , Gassmann Max , Thiersch Markus
Published: August 2022

Erythropoietin receptor (EPOR) is widely expressed in healthy and malignant tissues. In certain malignancies, EPOR stimulates tumor growth. In healthy tissues, EPOR controls processes other than erythropoiesis, including mitochondrial metabolism. We hypothesized that EPOR also controls the mitochondrial metabolism in cancer cells. To test this hypothesis, we generated EPOR-knockdown cancer cells to grow tumor xenografts in mice and analyzed tumor cellular respiration via high-resolution respirometry. Furthermore, we analyzed cellular respiratory control, mitochondrial content, and regulators of mitochondrial biogenesis in vivo and in vitro in different cancer cell lines. Our results show that EPOR controls tumor growth and mitochondrial biogenesis in tumors by controlling the levels of both, pAKT and inducible NO synthase (iNOS). Furthermore, we observed that the expression of EPOR is associated with the expression of the mitochondrial marker VDAC1 in tissue arrays of lung cancer patients, suggesting that EPOR indeed helps to regulate mitochondrial biogenesis in tumors of cancer patients. Thus, our data imply that EPOR not only stimulates tumor growth but also regulates tumor metabolism and is a target for direct intervention against progression.
Quantitative proteomics on the cerebrospinal fluid of hydrocephalus in neonatal bacterial meningitis
Authors: Chen Juncao , Huang Weiben , Zhang Hong , Peng Xiangwen , Yang Jun , Yang Yong , Su Jinzhen , Wang Siyao , Zhou Wei
Published: August 2022

Objective

Hydrocephalus in bacterial meningitis (BM) is a devastating infectious neurological disease and the proteins and pathways involved in its pathophysiology are not fully understood.

Materials and methods

Label-free quantitative (LFQ) proteomics analyses was used to identify differentially expressed proteins (DEPs) in cerebrospinal fluid (CSF) samples from infants with hydrocephalus and bacterial meningitis (HBM group, N = 8), infants with bacterial meningitis (BM group, N = 9); and healthy infants (N group, N = 11). Bioinformatics analysis was subsequently performed to investigate Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enriched signaling pathways of these DEPs. Six proteins (AZU1, COX4I1, EDF1, KRT31, MMP12, and PRG2) were selected for further validation via enzyme-linked immunosorbent assay (ELISA).

Results

Compared with BM group and N group, HBM group had a higher whole CSF protein level (5.6 ± 2.7 vs. 1.7 ± 1.0 vs. 1.2 ± 0.5 g/l) and lower whole CSF glucose level (0.8 ± 0.6 vs. 1.8 ± 0.7 vs. 3.3 ± 0.8 mmol/l) (both P < 0.05). Over 300 DEPs were differentially expressed in HBM group compared with BM group and BM compared with N group, of which 78% were common to both. Cluster analysis indicated that the levels of 226 proteins were increased in BM group compared with N group and were decreased in HBM group compared with BM group. Bioinformatics analysis indicated the involvement of the cell adhesion, immune response and extracellular exosome signaling were significantly enriched in HBM compared with BM group and BM compared with N group. 267 DEPs were identified between HBM group with N group, KEGG analysis indicated that DEPs mainly involved in filament cytoskeleton and immune response. The ELISA results further verified that the expression levels of AZU1 were significantly different from among three groups (both P < 0.05).

Conclusion

This is the first reported characterization of quantitative proteomics from the CSF of infants with HBM. Our study also demonstrated that AZU1 could be a potential biomarker for the diagnosis of hydrocephalus in bacterial meningitis.
Protective effect of quercetin on cadmium-induced renal apoptosis through cyt-c/caspase-9/caspase-3 signaling pathway
Authors: Huang Ruxue , Ding Lulu , Ye Ying , Wang Ke , Yu Wenjing , Yan Bingzhao , Liu Zongping , Wang Jicang
Published: August 2022

Cadmium (Cd), a heavy metal, has harmful effects on animal and human health, and it can also obviously induce cell apoptosis. Quercetin (Que) is a flavonoid compound with antioxidant and other biological activities. To investigate the protective effect of Que on Cd-induced renal apoptosis in rats. 24 male SD rats were randomly divided into four groups. They were treated as follows: control group was administered orally with normal saline (10 ml/kg); Cd group was injected with 2 mg/kg CdCl2 intraperitoneally; Cd + Que group was injected with 2 mg/kg CdCl2 and intragastric administration of Que (100 mg/kg); Que group was administered orally with Que (100 mg/kg). The experimental results showed that the body weight of Cd-exposed rats significantly decreased and the kidney coefficient increased. In addition, Cd significantly increased the contents of Blood Urea Nitrogen, Creatinine and Uric acid. Cd also increased the glutathione and malondialdehyde contents in renal tissues. The pathological section showed that Cd can cause pathological damages such as narrow lumen and renal interstitial congestion. Cd-induced apoptosis of kidney, which could activate the mRNA and protein expression levels of Cyt-c, Caspase-9 and Caspase-3 were significantly increased. Conversely, Que significantly reduces kidney damage caused by Cd. Kidney pathological damage was alleviated by Que. Que inhibited Cd-induced apoptosis and decreased Cyt-c, Caspase-9 and Caspase-3 proteins and mRNA expression levels. To sum up, Cd can induce kidney injury and apoptosis of renal cells, while Que can reduce Cd-induced kidney damage by reducing oxidative stress and inhibiting apoptosis. These results provide a theoretical basis for the clinical application of Que in the prevention and treatment of cadmium poisoning.
The relationship between different types of co-speech gestures and L2 speech performance
Authors: Ma Sai , Jin Guangsa
Published: August 2022

Co-speech gestures are closely connected to speech, but little attention has been paid to the associations between gesture and L2 speech performance. This study explored the associations between four types of co-speech gestures (namely, iconics, metaphorics, deictics, and beats) and the meaning, form, and discourse dimensions of L2 speech performance. Gesture and speech data were collected by asking 61 lower-intermediate English learners whose first language is Chinese to retell a cartoon clip. Results showed that all the four types of co-speech gestures had positive associations with meaning and discourse L2 speech measures but no association with form-related speech measures, except the positive association between metaphorics and the percentage of error-free clauses. The findings suggest that co-speech gestures may have a tighter connection with meaning construction in producing L2 speech.
The Global Burden of Diseases attributed to high low-density lipoprotein cholesterol from 1990 to 2019
Authors: Zheng Jia , Wang Jing , Zhang Yan , Xia Jiangliu , Guo Huilan , Hu Haiying , Shan Pengfei , Li Tianlang
Published: August 2022

Background

To demonstrate the real-word situation of burdens that are attributed to the risk factor of high low-density lipoprotein cholesterol (LDL-C) at the global, regional, national levels, among different age groups and between genders.

Methods

We analyzed data from the Global Burden of Disease study 2019 related to global deaths, disability-adjusted life years (DALYs), summary exposure value (SEV), average annual percentage change (AAPC), and observed to expected ratios (O/E ratios) attributable to high LDL-C from 1990 to 2019.

Results

Globally, in 2019, the total numbers of deaths and DALYs attributed to high LDL cholesterol were 1.47 and 1.41 times higher than that in 1990. The age-standardized deaths and DALYs rate was 1.45 and 1.70 times in males compared to females, while the age-standardized SEVs rate was 1.10 times in females compared to males. The deaths, DALYs, and SEV rates increased with age. In 2019, the highest age-standardized rates of both deaths and DALYs occurred in Eastern Europe while the lowest occurred in high-income Asia Pacific. High-income North America experienced a dramatic reduction of risk related to high LDL-C. Correlation analysis identified that the age-standardized SEV rate was positively correlated with Socio-demographic Index (SDI; r = 0.7753, P < 0.001). The average annual percentage change (AAPC) of age-standardized SEV rate decreased in the high SDI and high-middle SDI regions but increased in the middle SDI, low-middle SDI, and low SDI regions. High LDL-C mainly contributed to ischemic heart diseases.

Conclusion

High LDL-C contributed considerably to health burden worldwide. Males suffered worse health outcomes attributed to high LDL-C when compared to females. The burden attributed to high LDL-C increased with age. Lower SDI regions and countries experienced more health problem challenges attributed to high LDL-C as the result of social development and this should be reflected in policymaking.
Occupational stress and health risk of employees working in the garments sector of Bangladesh: An empirical study
Authors: Yuan Deli , Gazi Md. Abu Issa , Rahman Md. Alinoor , Dhar Bablu Kumar , Rahaman Md. Atikur
Published: August 2022

The present study was conducted with a view to examining the impact of occupational stress on employees' health risk. A total number of 350 garment employees (114 supervisors and 236 workers) were selected from 25 readymade garment factories of Dhaka, Narayanganj, and Gazipur industrial areas of Bangladesh on a random sampling basis. Occupational stress was estimated using an ERIs modified questionnaire; when self-reported health problems, work related information and socio-demographic information were obtained using face-to-face interviews using a pre-formed questionnaire. The survey was conducted for 2 years from January 2020 to December 2021 in Dhaka, Narayanganj and Gazipur districts where most of the garment industries in Bangladesh are located. All data were processed by using Statistical Package for Social Sciences (SPSS) and Decision Analyst Stats, Version 2.0. For analyzing data, suitable statistical tools such as two-way ANOVA, z-test, chi-square test, Pearson's product-moment correlation, stepwise multiple regressions, and descriptive statistics were used. The results of the present study reveal that the occupational stress had a significant positive influence on health risk. The findings also reveal that both the male and female employees perceived garment job highly stressful and risky for their health causes many dies and sickness, but it was higher among the female employees than their counterparts. Study suggests that due to major illness and diseases garments' employees are lacks of sound health that have to consider remedying for reducing occupational stress and health risk.
Erectile dysfunction: Is platelet-rich plasma the new frontier for treatment in patients with erectile dysfunction? A review of the existing evidence
Authors: Anastasiadis Eleni , Ahmed Razna , Khoja Abbas Khizar , Yap Tet
Published: August 2022

Erectile dysfunction (ED) is one of the commonest disorders in adult males and affects 12–19% of men of reproductive age. Only few studies have evaluated the impact of ED on men and couples with infertility—these studies report higher rates of ED in this sub-group of men compared to the general population, with the prevalence of ED in men diagnosed with male infertility ranging from 6.7 to 61.6%. Nevertheless, ED is considered a rare cause of male infertility, accounting for about 0.4–5% of all causes of male infertility. ED remains a poorly treated condition globally and current therapies, like oral medication, offer only temporary symptomatic relief and do not influence disease progression—patients are potentially on lifelong treatment, with ED worsening over time. In contrast, regenerative medicine may potentially reverse or halt the progression of ED processes. In this article, we review the evidence for intracavernosal injections of platelet-rich plasma (PRP) in the treatment of ED.
Transient high latitude geomagnetic response to rapid increases in solar wind dynamic pressure
Authors: Madelaire Michael , Laundal Karl M. , Reistad Jone P. , Hatch Spencer M. , Ohma Anders
Published: August 2022

Rapid changes in solar wind dynamic pressure can produce a transient geomagnetic response in the high latitude ionosphere. In this study we carry out a superposed epoch analysis of the geomagnetic response based on 2,058 events. The events are divided into 12 groups based on interplanetary magnetic field clock angle and dipole tilt and the magnetic perturbation field is modeled using spherical harmonics. We find that the high latitude transient current vortices associated with a sudden commencement are most clearly observed when the interplanetary magnetic field is northward during equinox and winter in the northern hemisphere. The high latitude geomagnetic response during northward interplanetary magnetic field is decomposed into a preliminary and main impulse. The preliminary impulse onset is 1–2 min prior to the onset of the low/mid latitude geomagnetic response and its rise time is 4–6 min. The main impulse onset is around 2 min after the low/mid latitude geomagnetic response and has a rise time of 6–11 min. When examining the change relative to pre-onset conditions a coherent transient geomagnetic response emerges for all IMF clock and dipole tilt angles. The current vortex associated with the main impulse on the dawnside appears at (9.3 ± 0.5 mlt, 64.8° ± 1.5° mlat) and moves westward with a velocity of 5 ± 1.4 km/s. The vortex on the duskside appears at (15.3 ± 0.9 mlt, 65.8° ± 2.5° mlat) and does not move significantly. In addition, the models were used to recreate the SMR index showing a significant mlt dependence on the magnetic perturbation above 40° mlat and below 10° mlat. The former is thought to be caused by high latitude ionospheric currents. The latter is potentially a combination of the event occurrence probability being skewed toward certain UT ranges for large dipole tilt angles and a UT dependence of the equatorial electrojet magnitude caused by the south atlantic magnetic anomaly.
The relationship between pedal force application technique and the ability to perform supramaximal pedaling cadences
Authors: Yamaguchi Yuta , Otsuka Mitsuo , Watanabe Kohei , Wada Naoki , Nishiyama Tetsunari
Published: August 2022

This study aimed to examine the relationship between the pedal force application technique under a specific competitive condition and the ability to perform steady-state pedaling at a supramaximal cadence during a special pedaling test. A total of 15 competitive male cyclists and 13 active, healthy men (novice cyclists, hereafter, novices) performed the pedaling technique test. The test imitated a road cycling competition condition (80% VO2 peak and a cadence of 90 rpm). Additionally, they performed a supramaximal cadence test that evaluated the ability to perform steady-state pedaling for an ultra-high cadence (range of 160–220 rpm) of 30 s stably with a 0.1 kgf. For the pedaling technique test, kinetic data were obtained by the pedal-shaped force platform at 1,000 Hz, and the pedaling technique was determined by the index of force effectiveness (IFE). For the supramaximal cadence test, kinematic data were obtained using a motion capture system at 200 Hz. The supramaximal pedaling cadence (Cmax) was determined by measuring exercise time and targeted pedaling cadence. The IFE was 48.0 ± 9.7% in cyclists and 32.0 ± 5.9% in novices. The Cmax was 215.5 ± 8.8 rpm in cyclists and 192.2 ± 13.0 rpm in novices. These values were significantly higher for cyclists than for novices. Cmax was moderately correlated with IFE (r = 0.64). No significant correlation was observed between Cmax and IFE for cyclists only; in contrast, a moderate correlation was observed between these parameters for novices only (r = 0.67). In conclusion, the pedal force application technique under a specific competitive condition is related to the ability to perform steady-state pedaling for supramaximal cadence during the test. Therefore, Cmax may be able to explain pedal force application techniques without the need for expensive devices for novices.
Myocarditis complicated by massive right ventricular thrombus and extensive pulmonary embolism: A case report
Authors: Jiang Xiao-juan , Zhang Wei-yi
Published: August 2022

An intracardiac thrombus may develop as a consequence of myocarditis, and in rare cases, a dominantly right ventricular thrombus develops, which may impair cardiac function and even cause life-threatening cardiovascular events. We report a 24-year-old man presented with recurrent episodes of palpitation and precordial discomfort after catching a cold 2 months ago. Transthoracic echocardiography (TTE) and computed tomography pulmonary angiogram (CTPA) revealed a mass attached to the apex of the right ventricle and extensive bilateral pulmonary artery emboli. There was no indication where the thrombi originated from in this young patient without any underlying disease except myocarditis. Pulmonary endarterectomy and embolectomy of pulmonary arteries and right ventricle were performed. Postoperative pathological results confirmed the presence of fibrinous necrosis and hemosiderin deposition. The formation of an intraventricular thrombus is closely related to myocarditis, which can affect individuals of all ages, but especially young people. Thus, patients with myocarditis should be closely monitored and followed up because of the increased risk of extensive thrombosis.
Evolutionary models predict potential mechanisms of escape from mutational meltdown
Authors: Bank Claudia , Schmitz Mark A. , Morales-Arce Ana Y.
Published: August 2022

Mutagenic drugs are promising candidates for the treatment of various RNA virus infections. Increasing the mutation rate of the virus leads to rapid accumulation of deleterious mutation load, which is proposed to ultimately result in extinction as described by the theoretical concepts of mutational meltdown and lethal mutagenesis. However, the conditions and potential mechanisms of viral escape from the effects of mutagenic drugs have not been conceptually explored. Here we apply a computational approach to quantify the population dynamics and genetics of a population under high mutation rates and discuss the likelihood of adaptation to a mutagenic drug by means of three proposed mechanisms: (1) a proportion of “traditional” beneficial mutations that increase growth/fitness, (2) a mutation rate modifier (i.e., evolution of resistance to the mutagenic drug) that reduces the mutation rate, and (3) a modifier of the distribution of fitness effects, which either decreases or increases deleterious effects of mutations (i.e., evolution of tolerance to the mutagenic drug). We track the population dynamics and genetics of evolving populations and find that successful adaptations have to appear early to override the increasing mutational load and rescue the population from its imminent extinction. We highlight that the observed stochasticity of adaptation, especially by means of modifiers of the distribution of fitness effects, is difficult to capture in experimental trials, which may leave potential dangers of the use of mutagenic treatments unexposed.
Inequity in the Distribution of Non-Communicable Disease Multimorbidity in Adults in South Africa: An Analysis of Prevalence and Patterns
Authors: Roomaney R. A. , van Wyk B. , Cois A. , Pillay-van Wyk V.
Published: August 2022

Objectives:The present study examined the prevalence and patterns of non-communicable disease multimorbidity by wealth quintile among adults in South Africa.

Methods:The South African National Income Dynamics Study Wave 5 was conducted in 2017 to examine the livelihoods of individuals and households. We analysed data in people aged 15 years and older (N = 27,042), including self-reported diagnosis of diabetes, stroke, heart disease and anthropometric measurements. Logistic regression and latent class analysis were used to analyse factors associated with multimorbidity and common disease patterns.

Results:Multimorbidity was present in 2.7% of participants. Multimorbidity was associated with increasing age, belonging to the wealthiest quintile group, increasing body mass index and being a current smoker. Having secondary education was protective against multimorbidity. Three disease classes of multimorbidity were identified: Diabetes and Hypertension; Heart Disease and Hypertension; and Stroke and Hypertension.

Conclusion:Urgent reforms are required to improve health systems responsiveness to mitigate inequity in multimorbidity patterns in the adult population of South Africa as a result of income inequality.
On (k,ψ)-Hilfer Fractional Differential Equations and Inclusions with Mixed (k,ψ)-Derivative and Integral Boundary Conditions
Authors: Ntouyas Sotiris K. , Ahmad Bashir , Nuchpong Cholticha , Tariboon Jessada
Published: August 2022

In this paper we study single-valued and multi-valued (k,ψ)-Hilfer-type boundary value problems of fractional order in (1,2], subject to nonlocal boundary conditions involving (k,ψ)-Hilfer-type derivative and integral operators. The results for single-valued case are established by using Banach and Krasnosel’skiĭ fixed point theorems as well as Leray–Schauder nonlinear alternative. In the multi-valued case, we establish an existence result for the convex valued right-hand side of the inclusion via Leray–Schauder nonlinear alternative for multi-valued maps, while the second one when the right-hand side has non-convex values is obtained by applying Covitz–Nadler fixed point theorem for multi-valued contractions. Numerical examples illustrating the obtained theoretical results are also presented.
Atomic Details of Biomineralization Proteins Inspiring Protein Design and Reengineering for Functional Biominerals
Authors: Di Costanzo Luigi Franklin
Published: August 2022

Biominerals are extraordinary materials that provide organisms with a variety of functions to support life. The synthesis of biominerals and organization at the macroscopic level is a consequence of the interactions of these materials with proteins. The association of biominerals and proteins is very ancient and has sparked a wealth of research across biological, medical and material sciences. Calcium carbonate, hydroxyapatite, and silica represent widespread natural biominerals. The atomic details of the interface between macromolecules and these biominerals is very intriguing from a chemical perspective, considering the association of chemical entities that are structurally different. With this review I provide an overview of the available structural studies of biomineralization proteins, explored from the Protein Data Bank (wwPDB) archive and scientific literature, and of how these studies are inspiring the design and engineering of proteins able to synthesize novel biominerals. The progression of this review from classical template proteins to silica polymerization seeks to benefit researchers involved in various interdisciplinary aspects of a biomineralization project, who need background information and a quick update on advances in the field. Lessons learned from structural studies are exemplary and will guide new projects for the imaging of new hybrid biomineral/protein superstructures at the atomic level.
Is There a Survival Benefit of Adjuvant Chemotherapy in Stage IC1 Epithelial Ovarian Cancer Patients? A Meta-Analysis
Authors: Pergialiotis Vasilios , Liatsou Efstathia , Rouvali Aggeliki , Haidopoulos Dimitrios , Efthymios Dimitrios , Liontos Michalis , Rodolakis Alexandros , Thomakos Nikolaos
Published: August 2022

The purpose of the present systematic review is to clarify whether adjuvant chemotherapy improves survival rates in women with stage IC1 ovarian cancer. We searched Medline, Scopus, Clinicaltrials.gov, EMBASE, Cochrane Central Register of Controlled Trials CENTRAL and Google Scholar. We considered comparative observational studies and randomized trials that investigated survival outcomes (progression-free (PFS) and overall survival (OS)) among women with intraoperative rupture of early-stage epithelial ovarian cancer who received adjuvant chemotherapy and those that did not. Eleven studies, which recruited 7556 patients, were included. The risk of bias was defined as moderate after assessment with the Risk of Bias in non-Randomized Trials tool. Meta-analysis was performed with RStudio. Seven studies investigated the impact of adjuvant chemotherapy on recurrence-free survival of patients experiencing intraoperative cyst rupture for otherwise stage I ovarian cancer. The outcome was not affected by the use of adjuvant chemotherapy as the effect estimate was not significant (HR 1.24, 95% CI 0.74, 2.04). The analysis of data from 5 studies similarly revealed that overall survival rates were comparable among the two groups (HR 0.75, 95% CI 0.54, 1.05). This meta-analysis did not detect any benefit from adjuvant chemotherapy for stage IC ovarian cancer patients with cyst rupture. However, conclusions from this investigation are limited by a study population which included multiple histologic subtypes, high and low grade tumors and incompletely staged patients.
Description and Use of Three-Dimensional Numerical Phantoms of Cardiac Computed Tomography Images
Authors: Vera Miguel , Bravo Antonio , Medina Rubén
Published: August 2022

The World Health Organization indicates the top cause of death is heart disease. These diseases can be detected using several imaging modalities, especially cardiac computed tomography (CT), whose images have imperfections associated with noise and certain artifacts. To minimize the impact of these imperfections on the quality of the CT images, several researchers have developed digital image processing techniques (DPIT) by which the quality is evaluated considering several metrics and databases (DB), both real and simulated. This article describes the processes that made it possible to generate and utilize six three-dimensional synthetic cardiac DBs or voxels-based numerical phantoms. An exhaustive analysis of the most relevant features of images of the left ventricle, belonging to a real CT DB of the human heart, was performed. These features are recreated in the synthetic DBs, generating a reference phantom or ground truth free of imperfections (DB1) and five phantoms, in which Poisson noise (DB2), stair-step artifact (DB3), streak artifact (DB4), both artifacts (DB5) and all imperfections (DB6) are incorporated. These DBs can be used to determine the performance of DPIT, aimed at decreasing the effect of these imperfections on the quality of cardiac images.
Fixed-Time Leader-Following Consensus Tracking Control for Nonliear Multi-Agent Systems under Jointly Connected Graph
Authors: Zhao Meng , Gu Chan , Zhao Le , Liu Yungang
Published: August 2022

This paper researches the fixed-time leader-following consensus problem for nonlinear multi-agent systems (MASs) affected by unknown disturbances under the jointly connected graph. In order to achieve control goal, this paper designs a fixed-time consensus protocol, which can offset the unknown disturbances and the nonlinear item under the jointly connected graph, simultaneously. In this paper, the states of multiple followers can converge to the state of the leader within a fixed time regardless of the initial conditions rather than just converging to a small neighborhood near the leader state. Finally, a simulation example is given to illustrate the theoretical result.
Voltage Rise Mitigation in PV Rich LV Distribution Networks Using DC/DC Converter Level Active Power Curtailment Method
Authors: Verma Pankaj , Katal Nitish , Sharma Bhisham , Chowdhury Subrata , Mehbodniya Abolfazl , Webber Julian L. , Bostani Ali
Published: August 2022

In low voltage (LV) distribution systems, the problem of overvoltage is common during the lower load intervals. This problem arises because of the high value of R/X ration of these systems. Many techniques are available in literature to cope up with this problem at the converter level; mostly these methods control the reactive or active power of the photovoltaic (PV) systems. However, there are certain restrictions and complications with the reactive power control of PV systems. Most of the active power control methods have been implemented at the inverter stage of the PV system, resulting in implementation complexities and excessive oversizing of the converter. Therefore, in this paper, a simple, de-rating based voltage control algorithm is proposed to overcome the problem of overvoltage. So far de-rating technique has been used to enable frequency support functions in PVs; in a first of its kind, de-rating technique is used here to control the voltages in PV rich LV distribution systems. The entire control is implemented on the dc/dc converter stage of the PV system and the inverter stage is kept untouched. The effectiveness of the control is verified by simulating a sample PV-rich three bus LV distribution system on the MATLAB software. The proposed control avoids the overvoltage by approx. 700 V for the best-case scenario.
Experimental Study on the Hydraulic Fracture Propagation in Inter-Salt Shale Oil Reservoirs
Authors: Shen Yunqi , Hu Zhiwen , Chang Xin , Guo Yintong
Published: August 2022

In response to the difficulty of fracture modification in inter-salt shale reservoirs and the unknown pattern of hydraulic fracture expansion, corresponding physical model experiments were conducted to systematically study the effects of fracturing fluid viscosity, ground stress and pumping displacement on hydraulic fracture expansion, and the latest supercritical CO2 fracturing fluid was introduced. The test results show the following. (1) The hydraulic fractures turn and expand when they encounter the weak surface of the laminae. The fracture pressure gradually increases with the increase in fracturing fluid viscosity, while the fracture pressure of supercritical CO2 is the largest and the fracture width is significantly lower than the other two fracturing fluids due to the high permeability and poor sand-carrying property. (2) Compared with the other two conventional fracturing fluids, under the condition of supercritical CO2 fracturing fluid, the increase in ground stress leads to the increase in inter-salt. (3) Compared with the other two conventional fracturing fluids, under the conditions of supercritical CO2 fracturing fluid, the fracture toughness of shale increases, the fracture pressure increases, and the fracture network complexity decreases as well. (4) With the increase in pumping displacement, the fracture network complexity increases, while the increase in the displacement of supercritical CO2 due to high permeability leads to the rapid penetration of inter-salt shale hydraulic fractures to the surface of the specimen to form a pressure relief zone; it is difficult to create more fractures with the continued injection of the fracturing fluid, and the fracture network complexity decreases instead.
Strike-Slip Fault Effects on Diversity of the Ediacaran Mound-Shoal Distribution in the Central Sichuan Intracratonic Basin, China
Authors: Wen Long , Ran Qi , Tian Weizhen , Liang Han , Zhong Yuan , Zou Yu , Su Chen , Wu Guanghui
Published: August 2022

The largest Precambrian gasfield in China has been found from the Ediacaran (Sinian) carbonates in the central Sichuan Basin. The deep ancient reservoirs were generally attributed to the high-energy mound-shoal body in the carbonate platform. However, there is still little understanding on the distribution of the mound-shoal bodies that hampers further gas exploitation from the deep subsurface. Based on the seismic data, a large strike-slip fault system was identified by new 3D seismic data in the central Sichuan Basin. Further, it was found that the strike-slip fault had some effects on the mound-shoal bodies of the Ediacaran Dengying Formation. First, the platform margin was divided by strike-slip faults into three distinct segments to show two weak-rimmed margins and one interbedded rimmed margin. Second, the platform margin could be offset or migrated with the strike-slip faults. Third, there is varied margin thickness across the strike-slip fault zone. In the inner platform, more carbonate mound-shoal bodies developed behind the weak-rimmed margin that was divided by the strike-slip fault zones. In addition, the mound-shoal bodies may be separated by faulted sag. Further, the mound-shoal bodies may have developed along the faulted higher position in one side of the strike-slip fault zone. These patterns of the mound-shoal bodies suggest that the strike-slip fault had an important role in the sedimentary microfacies’ diversity in the intracratonic carbonates. The low displacement of the strike-slip fault is chiefly responsible for a weaker controlling effect on the microfacies change in the intracratonic basin rather than other tectonic settings.
Comprehensive CFD Aerodynamic Simulation of a Sport Motorcycle
Authors: Wiński Krzysztof , Piechna Adam
Published: August 2022

Nowadays, aerodynamics is a key focal point in the vehicle design process. Beyond its direct impact on the performance of a vehicle, it also has significant effects on economics and safety. In the last decade numerical methods, mainly Computational Fluid Dynamics (CFD), have established themselves as a reliable tool that assists in the design process and complements classical tunnel tests. However, questions remain about the possible obtained accuracy, best practices and applied turbulence models. In this paper, we present a comprehensive study of motorcycle aerodynamics using CFD methods which, compared to the most common car aerodynamics analysis, has many specific features. The motorcycle, along with its rider, constitutes a shape with very complex aerodynamic properties. A detailed insight into the flow features is presented with detailed commentary. The front fairing, the front wheel and its suspension were identified as the main contributors to the aerodynamic drag of the motorcycle and its rider. The influence of rider position was also studied and identified as one of the most important elements when considering motorcycle aerodynamics. An extensive turbulence models study was performed to evaluate the accuracy of the most common Reynolds-averaged Navier–Stokes models and novel hybrid models, such as the Scale Adaptive Simulation and the Delayed Detached Eddy Simulation. Similar values of drag coefficients were obtained for different turbulence models with noticeable differences found for k−ϵ models. It was also observed that near-wall treatment affects the flow behaviour near the wheels and windshield but has no impact on the global aerodynamic parameters. In the summary, a discussion about the obtained results was set forth and a number of questions related to specifics of motorcycle CFD simulations were addressed.
Effects of a Combined Disinfection Method on Pseudomonas aeruginosa Biofilm in Freshwater Swimming Pool
Authors: Sigler Zekanović Melani , Begić Gabrijela , Medić Alan , Gobin Ivana , Tomić Linšak Dijana
Published: August 2022

Pool water must meet certain chemical and microbiological conditions to ensure high water quality and a safe environment for users. A recreational swimming pool treated with a combined disinfection method (chlorination and UV radiation) was monitored for 18 months. Selected chemical and microbiological parameters of the indoor freshwater pool were analyzed, and the in vivo presence of Pseudomonas aeruginosa biofilm was assessed and further correlated to the type of disinfection. P. aeruginosa isolated from biofilm was further examined to determine the effects of combined disinfection methods on the formation and destruction of mature biofilm in vitro. The in vitro application of the combined disinfection methods led to the inhibition of planktonic P. aeruginosa biofilm formation (68.9% compared to the control group) and were more effective in the partial destruction of mature biofilm than individual disinfection methods (from 25.4 to 26.3%). The obtained results indicate the better microbiological and chemical quality of pool water when combined disinfection was applied. Our results contribute to developing the optimization of pool water disinfection methods and biofilm control.