bluuebunny/crossref_metadata_2025 huggingface dataset:
Nuclear Factor κB-COX2 Pathway Activation in Non-myelinating Schwann Cells Is Necessary for the Maintenance of Neuropathic Pain in vivo
Authors: Xie Alison Xiaoqiao , Taves Sarah , McCarthy Ken
Published: January 2022

Chronic neuropathic pain leads to long-term changes in the sensitivity of both peripheral and central nociceptive neurons. Glial fibrillary acidic protein (GFAP)-positive glial cells are closely associated with the nociceptive neurons including astrocytes in the central nervous system (CNS), satellite glial cells (SGCs) in the sensory ganglia, and non-myelinating Schwann cells (NMSCs) in the peripheral nerves. Central and peripheral GFAP-positive cells are involved in the maintenance of chronic pain through a host of inflammatory cytokines, many of which are under control of the transcription factor nuclear factor κB (NFκB) and the enzyme cyclooxygenase 2 (COX2). To test the hypothesis that inhibiting GFAP-positive glial signaling alleviates chronic pain, we used (1) a conditional knockout (cKO) mouse expressing Cre recombinase under the hGFAP promoter and a floxed COX2 gene to inactivate the COX2 gene specifically in GFAP-positive cells; and (2) a tet-Off tetracycline transactivator system to suppress NFκB activation in GFAP-positive cells. We found that neuropathic pain behavior following spared nerve injury (SNI) significantly decreased in COX2 cKO mice as well as in mice with decreased glial NFκB signaling. Additionally, experiments were performed to determine whether central or peripheral glial NFκB signaling contributes to the maintenance of chronic pain behavior following nerve injury. Oxytetracycline (Oxy), a blood-brain barrier impermeable analog of doxycycline was employed to restrict transgene expression to CNS glia only, leaving peripheral glial signaling intact. Signaling inactivation in central GFAP-positive glia alone failed to exhibit the same analgesic effects as previously observed in animals with both central and peripheral glial signaling inhibition. These data suggest that the NFκB-COX2 signaling pathway in NMSCs is necessary for the maintenance of neuropathic pain in vivo.
Impact of 25-Hydroxy Vitamin D on White Matter Hyperintensity in Elderly Patients: A Systematic Review and Meta-Analysis
Authors: Zhao Yilei , Xu Jingfeng , Feng Zhan , Wang Jincheng
Published: January 2022

Some studies show that low serum vitamin D levels are associated with white matter hyperintensity (WMH), while other studies report no association. This meta-analysis aimed to investigate the presence of an association between serum 25-hydroxy vitamin D [25(OH)D] levels and WMH. PubMed, Embase, the Cochrane Library, CNKI, WANFANG, and VIP were searched for available papers published up to December 2020. The outcomes were the odds ratios (ORs) with 95% confidence intervals (CIs) for the association between different vitamin D statuses and WMH. All meta-analyses were performed using a random-effects model. Five studies (4393 patients) were included. Compared with sufficient 25(OH)D levels, 25(OH)D deficiency was not associated with WMH (OR = 1.67, 95%CI: 0.92–3.04; I2 = 70.2%, Pheterogeneity = 0.009), nor was 25(OH)D insufficiency (OR = 1.21, 95%CI: 0.89–1.65; I2 = 48.1%, Pheterogeneity = 0.103). A decrease of 25 nmol/L in 25(OH)D levels was associated with WMH (OR = 1.83, 95%CI: 1.34-2.49; I2 = 0%, Pheterogeneity= 0.512). The sensitivity analyses showed that the results were robust. 25(OH)D deficiency and insufficiency are not associated with WMH. A decrease of 25 nmol/L in 25(OH)D levels was associated with WMH, but this result will have to be confirmed. Prospective trials, both cross-sectional and longitudinal, are necessary to examine the association between 25(OH)D levels and WMH.
Large Vessel Occlusion in Patients With Minor Ischemic Stroke in a Population-Based Study. The Dijon Stroke Registry
Authors: Duloquin Gauthier , Crespy Valentin , Jakubina Pauline , Giroud Maurice , Vergely Catherine , Béjot Yannick
Published: January 2022

Introduction:

Strategy for the acute management of minor ischemic stroke (IS) with large vessel occlusion (LVO) is under debate, especially the benefits of mechanical thrombectomy. The frequency of minor IS with LVO among overall patients is not well established. This study aimed to assess the proportion of minor IS and to depict characteristics of patients according to the presence of LVO in a comprehensive population-based setting.

Methods:

Patients with acute IS were prospectively identified among residents of Dijon, France, using a population-based registry (2013–2017). All arterial imaging exams were reviewed to assess arterial occlusion. Minor stroke was defined as that with a National Institutes of Health Stroke Scale (NIHSS) score of <6. Proportion of patients with LVO was estimated in the minor IS population. The clinical presentation of patients was compared according to the presence of an LVO.

Results:

Nine hundred seventy-one patients were registered, including 582 (59.9%) patients with a minor IS. Of these patients, 23 (4.0%) had a LVO. Patients with minor IS and LVO had more severe presentation [median 3 (IQR 2–5) vs. 2 (IQR 1–3), p = 0.001] with decreased consciousness (13.0 vs. 1.6%, p<0.001) and cortical signs (56.5 vs. 30.8%, p = 0.009), especially aphasia (34.8 vs. 15.4%, p = 0.013) and altered item level of consciousness (LOC) questions (26.1 vs. 11.6%, p = 0.037). In multivariable analyses, only NIHSS score (OR = 1.45 per point; 95% CI: 1.11–1.91, p = 0.007) was associated with proximal LVO in patients with minor IS.

Conclusion:

Large vessel occlusion (LVO) in minor stroke is non-exceptional, and our findings highlight the need for emergency arterial imaging in any patients suspected of acute stroke, including those with minor symptoms because of the absence of obvious predictors of proximal LVO.
Case Report: Novel SLC9A6 Splicing Variant in a Chinese Boy With Christianson Syndrome With Electrical Status Epilepticus During Sleep
Authors: Liu Xiaorui , Xie Lingling , Fang Zhixu , Jiang Li
Published: January 2022

We investigated the existence and potential pathogenicity of a SLC9A6 splicing variant in a Chinese boy with Christianson Syndrome (CS), which was reported for the first time in China. Trio whole-exome sequencing (WES) was performed in the proband and his parents. Multiple computer prediction tools were used to evaluate the pathogenicity of the variant, and reverse transcription-polymerase chain reaction (RT-PCR) analysis and cDNA sequencing were performed to verify the RNA splicing results. The patient presented with characteristic features of CS: global developmental delay, seizures, absent speech, truncal ataxia, microcephaly, ophthalmoplegia, smiling face and hyperkinesis with electrical status epilepticus during sleep (ESES) detected in an electroencephalogram (EEG). A SLC9A6 splicing variant was identified by WES and complete skipping of exon 10 was confirmed by RT-PCR. This resulted in altered gene function and was predicted to be pathogenic. ESES observed early in the disease course is considered to be a significant feature of CS with the SLC9A6 variant. Combined genetic analysis at both the DNA and RNA levels is necessary to confirm the pathogenicity of this variant and its role in the clinical diagnosis of CS.
Does Oblique Effect Affect SSVEP-Based Visual Acuity Assessment?
Authors: Zheng Xiaowei , Xu Guanghua , Du Yuhui , Li Hui , Han Chengcheng , Tian Peiyuan , Li Zejin , Du Chenghang , Yan Wenqiang , Zhang Sicong
Published: January 2022

This study aimed to explore whether there was an effect on steady-state visual evoked potential (SSVEP) visual acuity assessment from the oblique effect or the stimulus orientation. SSVEPs were induced by seven visual stimuli, e.g., the reversal sinusoidal gratings with horizontal, two oblique, and vertical orientations, reversal checkerboards with vertical and oblique orientations, and oscillating expansion-contraction concentric-rings, at six spatial frequency steps. Ten subjects participated in the experiment. Subsequently, a threshold estimation criterion was used to determine the objective SSVEP visual acuity corresponding to each visual stimulus. Taking the SSVEP amplitude and signal-to-noise-ratio (SNR) of the fundamental reversal frequency as signal characteristics, both the SSVEP amplitude and SNR induced by the reversal sinusoidal gratings at 3.0 cpd among four stimulus orientations had no significant difference, and the same finding was also shown in the checkerboards between vertical and oblique orientation. In addition, the SSVEP visual acuity obtained by the threshold estimation criterion for all seven visual stimuli showed no significant difference. This study demonstrated that the SSVEPs induced by all these seven visual stimuli had a similarly good performance in evaluating visual acuity, and the oblique effect or the stimulus orientation had little effect on SSVEP response as well as the SSVEP visual acuity.
Association Between the EAT-Lancet Diet Pattern and Risk of Type 2 Diabetes: A Prospective Cohort Study
Authors: Xu Chenjie , Cao Zhi , Yang Hongxi , Hou Yabing , Wang Xiaohe , Wang Yaogang
Published: January 2022

Background:

The EAT-Lancet Commission has promulgated a sustainable dietary guideline and recommended that it was designed to improve the human health and support environmental sustainability.

Objective:

This research was designed to explore the association between this healthy diet pattern (EAT-Lancet diet pattern, EAT-LDP) and risk of type 2 diabetes (T2D).

Methods:

Between 2006 and 2010, a total of 59,849 participants from the UK Biobank without diabetes, cardiovascular disease, or cancers were included at baseline. The EAT-LDP score was constructed on the sum of 14 food components and then categorized into three tertiles. Multivariable Cox proportional hazards regression models were conducted to explore the association between EAT-LDP score and the risk of incident T2D. A mediation analysis was also implemented to disentangle the role of body mass index (BMI) and waist circumference in the relationship between EAT-LDP score and T2D.

Results:

During a median follow-up of 10 years, 2,461 incident T2D cases were recorded. In analyses that compared tertile 3 of the EAT-LDP score (highest) with tertile 1 (lowest), the hazard ratio (HR) for T2D was 0.81 (95% CI: 0.72–0.90) after adjusting for sociodemographic status and health-related factors. Participants who reported a one-point increase in the diet score were associated with a 6% decrease in risk of T2D (HR: 0.94, 95% CI: 0.91–0.97). A significant indirect association was observed between the EAT-LDP score and T2D (β: 0.66, 95% CI: 0.65–0.67), indicating that 44% of the association of EAT-LDP score with T2D was mediated by BMI. Additionally, 40% of the association of EAT-LDP score with T2D was mediated by waist circumference was also observed.

Conclusions:

Our findings indicate that a higher adherence to EAT-LDP contributes to lower risk of T2D. Further independent validation is needed to be conducted before applying the EAT-LDP to inform dietary guidelines.
A Predictor Combining Clinical and Genetic Factors for AML1-ETO Leukemia Patients
Authors: Yang Min , Zhao Bide , Wang Jinghan , Zhang Yi , Hu Chao , Liu Lixia , Qin Jiayue , Lou Feng , Cao Shanbo , Wang Chengcheng , Yu Wenjuan , Tong Hongyan , Meng Haitao , Huang Jian , Zhu Honghu , Jin Jie
Published: January 2022

Core Binding Factor (CBF)-AML is one of the most common somatic mutations in acute myeloid leukemia (AML). t(8;21)/AML1-ETO-positive acute myeloid leukemia accounts for 5-10% of all AMLs. In this study, we consecutively included 254 AML1-ETO patients diagnosed and treated at our institute from December 2009 to March 2020, and evaluated molecular mutations by 185-gene NGS platform to explore genetic co-occurrences with clinical outcomes. Our results showed that high KIT VAF(≥15%) correlated with shortened overall survival compared to other cases with no KIT mutation (3-year OS rate 26.6% vs 59.0% vs 69.6%, HR 1.50, 95%CI 0.78-2.89, P=0.0005). However, no difference was found in patients’ OS whether they have KIT mutation in two or three sites. Additionally, we constructed a risk model by combining clinical and molecular factors; this model was validated in other independent cohorts. In summary, our study showed that c-kit other than any other mutations would influence the OS in AML1-ETO patients. A proposed predictor combining both clinical and genetic factors is applicable to prognostic prediction in AML1-ETO patients.
Integrative Multi−Omics Analysis Reveals Candidate Biomarkers for Oral Squamous Cell Carcinoma
Authors: Wan Zhengqing , Xiong Haofeng , Tan Xian , Su Tong , Xia Kun , Wang Danling
Published: January 2022

Oral squamous cell carcinoma (OSCC) is one of the most common types of cancer worldwide. Due to the lack of early detection and treatment, the survival rate of OSCC remains poor and the incidence of OSCC has not decreased during the past decades. To explore potential biomarkers and therapeutic targets for OSCC, we analyzed differentially expressed genes (DEGs) associated with OSCC using RNA sequencing technology. Methylation−regulated and differentially expressed genes (MeDEGs) of OSCC were further identified via an integrative approach by examining publicly available methylomic datasets together with our transcriptomic data. Protein−protein interaction (PPI) networks of MeDEGs were constructed and highly connected hub MeDEGs were identified from these PPI networks. Subsequently, expression and survival analyses of hub genes were performed using The Cancer Genome Atlas (TCGA) database and the Gene Expression Profiling Interactive Analysis (GEPIA) online tool. A total of 56 upregulated MeDEGs and 170 downregulated MeDEGs were identified in OSCC. Eleven hub genes with high degree of connectivity were picked out from the PPI networks constructed by those MeDEGs. Among them, the expression level of four hub genes (CTLA4, CDSN, ACTN2, and MYH11) were found to be significantly changed in the head and neck squamous carcinoma (HNSC) patients. Three hypomethylated hub genes (CTLA4, GPR29, and TNFSF11) and one hypermethylated hub gene (ISL1) were found to be significantly associated with overall survival (OS) of HNSC patients. Therefore, these hub genes may serve as potential DNA methylation biomarkers and therapeutic targets of OSCC.
Prophylactic Central Neck Dissection for cN1b Papillary Thyroid Carcinoma: A Systematic Review and Meta-Analysis
Authors: Yan Xing-qiang , Zhang Zhen-zhen , Yu Wen-jie , Ma Zhao-sheng , Chen Min-long , Xie Bo-jian
Published: January 2022

Background

The value of prophylactic central neck dissection (PCND) for papillary thyroid carcinoma (PTC) with clinically evident lateral cervical lymph node metastases (cN1b) remains unclear. Therefore, a systematic review and meta-analysis was conducted to assess the efficacy and safety of PCND.

Methods

A comprehensive systematic search was conducted on PubMed, Web of Science, Cochrane library and Embase databases up to September 2021 to identify eligible studies. Controlled clinical trials assessing therapeutic effects and safety of PCND for cN1b PTC patients were included. The risk of bias for each cohort study was assessed using the Newcastle-Ottawa Scale (NOS). The primary outcomes were indexes related to the locoregional recurrence (LRR) and surgical complications. Review Manager software V5.4.0 was used for statistical analysis. A fixed effects model was adopted for the data without heterogeneity, otherwise a random effects model was used.

Results

We included 4 retrospective cohort studies, which comprised 483 PTC patients. There was no statistically significant difference in the central neck recurrence (CNR) (10.2% vs. 3.8%, relative risk (RR) = 1.82; 95%CI 0.90–3.67; P = 0.09), lateral neck recurrence (LNR) (5.1% vs. 7.7%, RR = 0.47; 95% CI 0.13–1.74; P = 0.26), and overall recurrence (OR) (18.9% vs. 16.9%, RR = 0.77; 95%CI 0.34–1.76; P = 0.54), between LND + PCND group and LND group. Simultaneously, PCND increased the risk of permanent hypoparathyroidism (11.4% vs. 4.5%, RR = 2.70, 95%CI 1.05–6.94; P = 0.04) and overall complications (17.0% vs. 5.3%, RR = 3.28; 95%CI 1.37–7.86; P = 0.008).

Conclusions

This meta-analysis showed that PCND did not have any advantage in preventing LRR for cN1b PTC. Meanwhile, PCND may result in the increased rate of surgical complications. However, the current evidence is limited and more clinical trials are still needed to further clarify the true role of PCND.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/, CRD42021281825.
Establishing Surgical Care Sustainability in Sub-Saharan Africa for Global Child Health: Insights From Pediatric Cardiac Surgical Capacity-Building Programs in Ethiopia and Côte d'Ivoire
Authors: Park Jayoung , Heo Jongho , Kim Woong-Han
Published: January 2022

The global surgery research team of the JW LEE Center for Global Medicine, Seoul National University College of Medicine, introduced team-based health workforce training programs for pediatric cardiac surgery in Ethiopia and Côte d'Ivoire. A team-based collaborative capacity-building model was implemented in both countries, and details of the program design and delivery were documented. The research team shared their experiences and identified achievements, lessons, and challenges for cardiac surgical interventions in Sub-Saharan Africa. Future directions were put forward to advance and strengthen the low-and middle-income countries “Safe Surgery.”
Path-dependent Vortex Switching in Ferroelectric Nanoplate Junctions Toward a Memory Device Concept
Authors: Xiong Weiming , Chen Weijin , Zheng Yue
Published: January 2022

Ferroelectric vortex has attracted much attention as a promising candidate for memories with high density and high stability. It is a crucial problem to precisely manipulate the vortex chirality in order to utilize it to store information. Nevertheless, so far, a practical and direct strategy for vortex switching is still lacking. Moreover, the strong coupling of chirality between neighboring vortices in continuous systems like superlattices limits the application of ferroelectric-vortex-based memories. Here, we design a ferroelectric nanoplate junction to break the strong coupling between neighboring vortices. Phase-field simulation results demonstrate that the vortex chirality of the nanoplates could be efficiently tuned by sweeping local electric and thermal fields in the nanoplate junction. More importantly, the weak coupling between two neighboring nanoplates through the intermediate junction brings a deterministic vortex switching behavior. Based on this, we propose a concept of vortex memory devices. Our study provides an effective way to control the vortex chirality and suggests an opportunity for designing new memory devices based on ferroelectric vortex.
Monitoring Performance in Show Jumping Horses: Validity of Non-specific and Discipline-specific Field Exercise Tests for a Practicable Assessment of Aerobic Performance
Authors: Kirsch Katharina , Fercher Christina , Horstmann Stephanie , von Reitzenstein Caroline , Augustin Julia , Lagershausen Henrike
Published: January 2022

Show jumping is a highly specialized equestrian discipline that requires technical skill but also power and fitness. Monitoring the horses’ aerobic performance is therefore essential in order to verify whether the training has induced the desired cardiovascular and muscular adaptations. This study therefore aimed at evaluating the validity of non-specific and discipline-specific field exercise tests for objective evaluation of aerobic performance in show jumpers. For this purpose, data obtained from horses competing at Junior and Young Rider level during show jumping competitions as well as field exercise tests were retrospectively analyzed. The effect of the level of difficulty, the horses’ age, the penalty score and the horses’ previous level of performance on blood lactate concentrations after show jumping competitions (100 observations in 49 horses) was evaluated by linear mixed effects models (horse as random effect). Estimated marginal means significantly increased from 140 (4.1 mmol/L) to 150 cm (5.2 mmol/L) classes (P= 0.02). Furthermore, post-exercise lactate values significantly increased with the horses’ age (P= 0.001). Another group of 12 horses performed a standardized incremental field exercise test on a track (SETtrack), a standardized show jumping course (SETcourse) and a standardized grid exercise (SETgrid) each on three consecutive days. Indices of aerobic performance, derived from the SETtrack[velocity at a heart rate of 140 bpm (V140) and at a lactate concentration of 2 mmol/L (VLa2)] were highly correlated with heart rate (V140: r = −0.75,P= 0.005; VLa2: r =−0.66,P= 0.02) and lactate (V140: r = −0.73,P= 0.02; VLa2: r = −0.72,P= 0.02) in response to SETcourseas well as heart rate during SETgrid(V140: r = −0.73,P= 0.02; VLa2: r = −0.76,P= 0.01). Subjective rating of muscular fatigue was significantly correlated to the mean heart rate during SETcourse(r = −0.64,P= 0.05) and SETgrid(r = −0.74,P= 0.02) but not to the aerobic indices calculated from SETtrack. Besides non-specific incremental field tests, performance monitoring in show jumpers should therefore also include discipline-specific tests that more closely reflect the internal load induced by show jumping competitions.
S1-bZIP Transcription Factors Play Important Roles in the Regulation of Fruit Quality and Stress Response
Authors: Wang Hong , Zhang Yunting , Norris Ayla , Jiang Cai-Zhong
Published: January 2022

Sugar metabolism not only determines fruit sweetness and quality but also acts as signaling molecules to substantially connect with other primary metabolic processes and, therefore, modulates plant growth and development, fruit ripening, and stress response. The basic region/leucine zipper motif (bZIP) transcription factor family is ubiquitous in eukaryotes and plays a diverse array of biological functions in plants. Among the bZIP family members, the smallest bZIP subgroup, S1-bZIP, is a unique one, due to the conserved upstream open reading frames (uORFs) in the 5′ leader region of their mRNA. The translated small peptides from these uORFs are suggested to mediate Sucrose-Induced Repression of Translation (SIRT), an important mechanism to maintain sucrose homeostasis in plants. Here, we review recent research on the evolution, sequence features, and biological functions of this bZIP subgroup. S1-bZIPs play important roles in fruit quality, abiotic and biotic stress responses, plant growth and development, and other metabolite biosynthesis by acting as signaling hubs through dimerization with the subgroup C-bZIPs and other cofactors like SnRK1 to coordinate the expression of downstream genes. Direction for further research and genetic engineering of S1-bZIPs in plants is suggested for the improvement of quality and safety traits of fruit.
Assessing Patient Preferences: Examination of the German Cooper-Norcross Inventory of Preferences
Authors: Heinze Peter Eric , Weck Florian , Kühne Franziska
Published: January 2022

Despite the positive effects of including patients’ preferences into therapy on psychotherapy outcomes, there are still few thoroughly validated assessment tools at hand. We translated the 18-item Cooper-Norcross Inventory of Preferences (C-NIP) into German and aimed at replicating its factor structure. Further, we investigated the reliability of the questionnaire and its convergence with trait measures. A heterogeneous sample of N = 969 participants took part in our online survey. Performing ESEM models, we found acceptable model fit for a four-factor structure similar to the original factor structure. Furthermore, we propose an alternative model following the adjustment of single items. The German C-NIP showed acceptable to good reliability, as well as small correlations with Big-Five personality traits, trait and attachment anxiety, locus of control, and temporal focus. However, we recommend further replication of the factor structure and further validation of the C-NIP.
Why Do Children Who Solve False Belief Tasks Begin to Find True Belief Control Tasks Difficult? A Test of Pragmatic Performance Factors in Theory of Mind Tasks
Authors: Schidelko Lydia P. , Huemer Michael , Schröder Lara M. , Lueb Anna S. , Perner Josef , Rakoczy Hannes
Published: January 2022

The litmus test for the development of a metarepresentational Theory of Mind is the false belief (FB) task in which children have to represent how another agent misrepresents the world. Children typically start mastering this task around age four. Recently, however, a puzzling finding has emerged: Once children master the FB task, they begin to fail true belief (TB) control tasks. Pragmatic accounts assume that the TB task is pragmatically confusing because it poses a trivial academic test question about a rational agent’s perspective; and we do not normally engage in such discourse about subjective mental perspectives unless there is at least the possibility of error or deviance. The lack of such an obvious possibility in the TB task implicates that there might be some hidden perspective difference and thus makes the task confusing. In the present study, we test the pragmatic account by administering to 3- to 6-year-olds (N= 88) TB and FB tasks and structurally analogous true and false sign (TS/FS) tasks. The belief and sign tasks are matched in terms of representational and metarepresentational complexity; the crucial difference is that TS tasks do not implicate an alternative non-mental perspective and should thus be less pragmatically confusing than TB tasks. The results show parallel and correlated development in FB and FS tasks, replicate the puzzling performance pattern in TB tasks, but show no trace of this in TS tasks. Taken together, these results speak in favor of the pragmatic performance account.
Social Media Use and Misinformation Among Asian Americans During COVID-19
Authors: Chong Stella K. , Ali Shahmir H. , Ðoàn Lan N. , Yi Stella S. , Trinh-Shevrin Chau , Kwon Simona C.
Published: January 2022

Social media has been crucial for seeking and communicating COVID-19 information. However, social media has also promulgated misinformation, which is particularly concerning among Asian Americans who may rely on in-language information and utilize social media platforms to connect to Asia-based networks. There is limited literature examining social media use for COVID-19 information and the subsequent impact of misinformation on health behaviors among Asian Americans. This perspective reviews recent research, news, and gray literature to examine the dissemination of COVID-19 misinformation on social media platforms to Chinese, Korean, Vietnamese, and South Asian Americans. We discuss the linkage of COVID-19 misinformation to health behaviors, with emphasis on COVID-19 vaccine misinformation and vaccine decision-making in Asian American communities. We then discuss community- and research-driven responses to investigate misinformation during the pandemic. Lastly, we propose recommendations to mitigate misinformation and address the COVID-19 infodemic among Asian Americans.
Free-Living User Perspectives on Musculoskeletal Pain and Patient-Reported Mobility With Passive and Powered Prosthetic Ankle-Foot Components: A Pragmatic, Exploratory Cross-Sectional Study
Authors: Kannenberg Andreas , Morris Arri R. , Hibler Karl D.
Published: January 2022

Introduction

Studies with a powered prosthetic ankle-foot (PwrAF) found a reduction in sound knee loading compared to passive feet. Therefore, the aim of the present study was to determine whether anecdotal reports on reduced musculoskeletal pain and improved patient-reported mobility were isolated occurrences or reflect a common experience in PwrAF users.

Methods

Two hundred and fifty individuals with transtibial amputation (TTA) who had been fitted a PwrAF in the past were invited to an online survey on average sound knee, amputated side knee, and low-back pain assessed with numerical pain rating scales (NPRS), the PROMIS Pain Interference scale, and the PLUS-M for patient-reported mobility in the free-living environment. Subjects rated their current foot and recalled the ratings for their previous foot. Recalled scores were adjusted for recall bias by clinically meaningful amounts following published recommendations. Statistical comparisons were performed using Wilcoxon's signed rank test.

Results

Forty-six subjects, all male, with unilateral TTA provided data suitable for analysis. Eighteen individuals (39%) were current PwrAF users, whereas 28 subjects (61%) had reverted to a passive foot. After adjustment for recall bias, current PwrAF users reported significantly less sound knee pain than they recalled for use of a passive foot (−0.5 NPRS, p = 0.036). Current PwrAF users who recalled sound knee pain ≥4 NPRS with a passive foot reported significant and clinically meaningful improvements in sound knee pain (−2.5 NPRS, p = 0.038) and amputated side knee pain (−3 NPRS, p = 0.042). Current PwrAF users also reported significant and clinically meaningful improvements in patient-reported mobility (+4.6 points PLUS-M, p = 0.016). Individuals who had abandoned the PwrAF did not recall any differences between the feet.

Discussion

Current PwrAF users reported significant and clinically meaningful improvements in patient-reported prosthetic mobility as well as sound knee and amputated side knee pain compared to recalled mobility and pain with passive feet used previously. However, a substantial proportion of individuals who had been fitted such a foot in the past did not recall improvements and had reverted to passive feet. The identification of individuals with unilateral TTA who are likely to benefit from a PwrAF remains a clinical challenge and requires further research.
Speed Dating with Voice User Interfaces: Understanding How Families Interact and Perceive Voice User Interfaces in a Group Setting
Authors: Ostrowski Anastasia K. , Fu Jenny , Zygouras Vasiliki , Park Hae Won , Breazeal Cynthia
Published: January 2022

As voice-user interfaces (VUIs), such as smart speakers like Amazon Alexa or social robots like Jibo, enter multi-user environments like our homes, it is critical to understand how group members perceive and interact with these devices. VUIs engage socially with users, leveraging multi-modal cues including speech, graphics, expressive sounds, and movement. The combination of these cues can affect how users perceive and interact with these devices. Through a set of three elicitation studies, we explore family interactions (N = 34 families, 92 participants, ages 4–69) with three commercially available VUIs with varying levels of social embodiment. The motivation for these three studies began when researchers noticed that families interacted differently with three agents when familiarizing themselves with the agents and, therefore, we sought to further investigate this trend in three subsequent studies designed as a conceptional replication study. Each study included three activities to examine participants’ interactions with and perceptions of the three VUIS in each study, including an agent exploration activity, perceived personality activity, and user experience ranking activity. Consistent for each study, participants interacted significantly more with an agent with a higher degree of social embodiment, i.e., a social robot such as Jibo, and perceived the agent as more trustworthy, having higher emotional engagement, and having higher companionship. There were some nuances in interaction and perception with different brands and types of smart speakers, i.e., Google Home versus Amazon Echo, or Amazon Show versus Amazon Echo Spot between the studies. In the last study, a behavioral analysis was conducted to investigate interactions between family members and with the VUIs, revealing that participants interacted more with the social robot and interacted more with their family members around the interactions with the social robot. This paper explores these findings and elaborates upon how these findings can direct future VUI development for group settings, especially in familial settings.
Soft Robotic Deployable Origami Actuators for Neurosurgical Brain Retraction
Authors: Amadeo Tomas , Van Lewen Daniel , Janke Taylor , Ranzani Tommaso , Devaiah Anand , Upadhyay Urvashi , Russo Sheila
Published: January 2022

Metallic tools such as graspers, forceps, spatulas, and clamps have been used in proximity to delicate neurological tissue and the risk of damage to this tissue is a primary concern for neurosurgeons. Novel soft robotic technologies have the opportunity to shift the design paradigm for these tools towards safer and more compliant, minimally invasive methods. Here, we present a pneumatically actuated, origami-inspired deployable brain retractor aimed at atraumatic surgical workspace generation inside the cranial cavity. We discuss clinical requirements, design, fabrication, analytical modeling, experimental characterization, and in-vitro validation of the proposed device on a brain model.
Evolutionary Inverse Material Identification: Bespoke Characterization of Soft Materials Using a Metaheuristic Algorithm
Authors: Di Lecce Michele , Onaizah Onaizah , Lloyd Peter , Chandler James H. , Valdastri Pietro
Published: January 2022

The growing interest in soft robotics has resulted in an increased demand for accurate and reliable material modelling. As soft robots experience high deformations, highly nonlinear behavior is possible. Several analytical models that are able to capture this nonlinear behavior have been proposed, however, accurately calibrating them for specific materials and applications can be challenging. Multiple experimental testbeds may be required for material characterization which can be expensive and cumbersome. In this work, we propose an alternative framework for parameter fitting established hyperelastic material models, with the aim of improving their utility in the modelling of soft continuum robots. We define a minimization problem to reduce fitting errors between a soft continuum robot deformed experimentally and its equivalent finite element simulation. The soft material is characterized using four commonly employed hyperelastic material models (Neo Hookean; Mooney–Rivlin; Yeoh; and Ogden). To meet the complexity of the defined problem, we use an evolutionary algorithm to navigate the search space and determine optimal parameters for a selected material model and a specific actuation method, naming this approach as Evolutionary Inverse Material Identification (EIMI). We test the proposed approach with a magnetically actuated soft robot by characterizing two polymers often employed in the field: Dragon Skin™ 10 MEDIUM and Ecoflex™ 00-50. To determine the goodness of the FEM simulation for a specific set of model parameters, we define a function that measures the distance between the mesh of the FEM simulation and the experimental data. Our characterization framework showed an improvement greater than 6% compared to conventional model fitting approaches at different strain ranges based on the benchmark defined. Furthermore, the low variability across the different models obtained using our approach demonstrates reduced dependence on model and strain-range selection, making it well suited to application-specific soft robot modelling.
Ablutionary Urbanisms: Water Infrastructures, Cultural Production and Charitable Dispensation
Authors: Muller Brook
Published: January 2022

With interest in advancing inclusive urban landscapes and guided by principles of social and cultural sustainability, this essay speculates as to localized water infrastructures as “ablutionary urbanisms,” important forms of contemporary design expression in a context of rapid growth, widening inequalities, climate change and lack of resilience. It derives inspiration from vernacular precedents in advocating for an integrated, decentralized approach to addressing current urban water challenges. It explores the contemporary relevance of the sabil, a prominent civic feature of Islamic cities intended for the charitable dispensation of water. More specifically, this essay considers the contemporary relevance and potency of the sabil-kuttab, a hybrid building type unique to the city of Cairo in which a school (kuttab) sits atop a sabil. Such a type offers helpful guidance in devising principles and precepts relevant to contemporary infrastructural design in that: (1) it offers encouragement to advocate for distributed urban water systems as civically prominent elements of cities, particularly as these systems combine with other important community-focused programmatic features; and (2) given a reimagining of urban water systems as critical forms of cultural production, it offers encouragement for interdisciplinary teams to commit to the task of infrastructure planning as a promising locus of integrative design.
Non-Linear Damping of Surface Alfvén Waves Due to Uniturbulence
Authors: Ismayilli Rajab , Van Doorsselaere Tom , Goossens Marcel , Magyar Norbert
Published: January 2022

This investigation is concerned with uniturbulence associated with surface Alfvén waves that exist in a Cartesian equilibrium model with a constant magnetic field and a piece-wise constant density. The surface where the equilibrium density changes in a discontinuous manner are the source of surface Alfvén waves. These surface Alfvén waves create uniturbulence because of the variation of the density across the background magnetic field. The damping of the surface Alfvén waves due to uniturbulence is determined using the Elsässer formulation. Analytical expressions for the wave energy density, the energy cascade, and the damping time are derived. The study of uniturbulence due to surface Alfvén waves is inspired by the observation that (the fundamental radial mode of) kink waves behave similarly to surface Alfvén waves. The results for this relatively simple case of surface Alfvén waves can help us understand the more complicated case of kink waves in cylinders. We perform a series of 3D ideal MHD simulations for a numerical demonstration of the non-linearly self-cascading model of unidirectional surface Alfvén waves using the code MPI-AMRVAC. We show that surface Alfvén waves damping time in the numerical simulations follows well our analytical prediction for that quantity. Analytical theory and the simulations show that the damping time is inversely proportional to the amplitude of the surface Alfvén waves and the density contrast. This unidirectional cascade may play a role in heating the coronal plasma.
Girls and Young Women in Community Sport: A South Australian Perspective
Authors: Drummond Murray , Drummond Claire , Elliott Sam , Prichard Ivanka , Pennesi Jamie-Lee , Lewis Lucy K. , Bailey Christopher , Bevan Nadia
Published: January 2022

Girls' and young women's engagement and disengagement in physical activity has been well documented in Western culture. Sport plays a pivotal role in the development of behaviours that promote physical activity, particularly through commitment to team and individual goal attainment, socialisation, and feelings of belonging and self-identity. Community sport in Australia is the dominant pathway into state, national, and elite international competition. The importance of community sport in the lives of girls and young women cannot be overstated, irrespective of individual long-term sporting goals. Indeed, the dropout rate of girls in sports, like many other western cultures is significant and is certainly disproportionate to the numbers of boys who drop out. The present study aims to examine the intrapersonal, interpersonal, and environmental influences on community sporting pathways for girls and young women. Using a mixed-methods design, we include survey data from 2,189 high-school students (aged 12–18 years) and focus group and individual interview data from a subset of 37 high-school students, parents, and teachers, across metropolitan Adelaide, South Australia. The study included an examination of sporting practises and insights of male sport participants from the same age groups to juxtapose the findings and provide a more comprehensive understanding of girls' and young women's community sporting involvement. Parents and teachers were also included within the participant cohort to provide a comprehensive perspective. The results highlight the challenges that girls face with respect to engagement and disengagement in sport and particular points throughout their adolescent years. Recommendations are provided to help mitigate potential attrition of girls in sport in the future.
Evaluation of COVID-19 Restrictions on Distance Runners' Training Habits Using Wearable Trackers
Authors: Chan Zoe Y. S. , Peeters Rhys , Cheing Gladys , Ferber Reed , Cheung Roy T. H.
Published: January 2022

The COVID-19 pandemic caused widespread disruption to many individuals' lifestyles. Social distancing restrictions implemented during this global pandemic may bring potential impact on physical activity habits of the general population. However, running is one of the most popular forms of physical activity worldwide and one in which it could be maintained even during most COVID-19 restrictions. We aimed to determine the impact of COVID-19 restrictions on runners' training habits through analyzing the training records obtained from their GPS enabled wearable trackers. Retrospective and prospective data were collected from an online database (https://wetrac.ucalgary.ca). Runners' training habits, including frequency, intensity and duration of training, weekly mileage and running locations were analyzed and compared 9 months before and after the start of COVID-19 restrictions in March 2020. We found that runners ran 3 km per week more (p = 0.05, Cohen's d = 0.12) after the start of COVID-19 restrictions, and added 0.3 training sessions per week (p = 0.03, Cohen's d = 0.14). Moreover, runners ran an additional 0.4 sessions outdoors (p < 0.01, Cohen's d = 0.21) but there was no significant change in the intensity or duration of training sessions. Our findings suggested that runners adopted slightly different training regimen as a result of COVID-19 restrictions. Our results described the collective changes, irrespective of differences in response measures adopted by various countries or cities during the COVID-19 pandemic.
Fresh-Cut Vegetables Processing: Environmental Sustainability and Food Safety Issues in a Comprehensive Perspective
Authors: Raffo Antonio , Paoletti Flavio
Published: January 2022

The fresh-cut industry supplies the food market with healthy fresh fruit and vegetables and, in that way, may contribute to improve the nutritional status of the general population. On the other hand, over the last few years increasing concerns have been raised regarding the environmental impact of the fresh-cut industry, human health risks from exposure to disinfection by-products found in fresh-cut products and chlorine-based disinfection treatments during produce processing. This review provides a comprehensive view of the main interlinked aspects related to food safety and environmental impact of processing of fresh-cut vegetables. Advantages and downsides of the mainstream disinfection strategy, based on the use of chlorine-related disinfecting agents, along with some alternative treatments close to a wide commercial application, are discussed. Limitation in the application of these strategies to processing of organic fresh-cut produce are also highlighted, examining the specific environmental and food safety problems in the organic sector. Areas where lack of available information hinders at present a clear understanding of priorities of research and action are pointed out. Innovative conceptual tools are proposed to address these multiple and interlinking issues and to overcome limitations of currently available technologies. A comprehensive and multidisciplinary approach is suggested to move toward a more safe and environmentally sustainable production of fresh-cut products.
Long-Term Follow-Up of Restorations of Equine Cheek Teeth Infundibula (2006–2017)
Authors: Pearce Christopher J. , Brooks Nicky
Published: January 2022

Background:

Caries of the infundibula of equine cheek teeth can lead to significant dental disease including increased attritional wear, pulpar and apical disease, secondary sinusitis, and dental fracture. Restorations of cavities of equine cheek teeth infundibula have been performed since 1889. Recent advances in dental materials, instrumentation, and techniques have facilitated the use of dental restoration techniques by equine veterinary practitioners. No studies to date have demonstrated the safety or efficacy of restorations of equine cheek teeth infundibula.

Objectives:

To assess the long-term results of restorations of equine cheek teeth affected by infundibular caries, to report on the safety of the procedure, and to give guidelines for future restorative therapies.

Study Design:

Retrospective analysis of results of clinical and oroscopic examination of horses that underwent infundibular restoration procedures between 2006 and 2017.

Methods:

A total of 223 infundibula in 185 maxillary cheek teeth in 92 horses were restored using a variety of dental materials including glass ionomer cement, flowable and compactible resin composites. The time between restoration and re-examination was recorded along with findings of clinical signs in the interim, restorative material loss, and any further pathological changes of the teeth including caries progression, fracture, or apical disease. Follow-up examinations were performed over two study periods 2006–2012 and in 2017.

Results:

Over the full study period, 99% of treated horses available for follow-up examinations had no adverse clinical signs or developed any abnormalities of restored teeth observable on oroscopic examination. Of horses re-examined, 83% of restorations were shown to have minimal or no loss of the restoration material, with occlusal surface wear visibly comparable to other adjacent maxillary teeth. Statistical analysis showed success of the procedure was related to the restorative material used, the restoration technique, and the caries grade present at the time of restoration (grade 3 is more successful than grade 2).

Main Limitations:

There are no case controls in this study and therefore it is not clear if restoration of equine infundibula is a consistently beneficial procedure, or at which grade of caries progression restorations should be performed for optimum benefit. The procedures were not re-examined at consistent regular times creating some difficulties in standardizing results. Re-examinations of treated horses did not consistently include radiography or computed tomography and therefore some apical changes may have occurred in treated teeth without visual oroscopic or external clinical signs.

Conclusion:

Restoration of equine infundibula using materials developed for human dentistry including flowable resin composites is a safe and long-lasting procedure and appears to prevent the development of further pathological changes including apical infection and dental fracture.