bluuebunny/crossref_metadata_2025 huggingface dataset:
The impact of publications in core public choice journals: an analysis of institution rankings
Authors: Mixon Franklin G. , Upadhyaya Kamal P.
Published: June 2021

This study examines the impact of research published in the two core public choice journals – Public Choice and the Journal of Public Finance and Public Choice – during the five-year period from 2010 through 2014. Scholars representing almost 400 universities contributed impactful research to these journals over this period, allowing us to rank institutions on the basis of citations to this published research. Our work indicates that public choice scholarship emanating from non-US colleges and universities has surged, with the University of Göttingen, University of Linz, Heidelburg University, University of Oxford, University of Konstanz, Aarhus University, University of Groningen, Paderborn University, University of Minho and University of Cambridge occupying ten of the top 15 positions in our worldwide ranking. Even so, US-based institutions still maintain a lofty presence, with Georgetown University, Emory University, the University of Illinois and George Mason University each holding positions among the top five institutions worldwide.
Shroud of Turin ultraviolet light images: color and information content
Authors: McAvoy Thomas
Published: June 2021

This paper builds on an earlier paper [Appl. Opt. 58, 6958 (2019)APOPAI0003-693510.1364/AO.58.006958] that analyzed web ultraviolet light (uv) photographs of the Shroud of Turin. In the earlier paper, it is shown that the Shroud exhibits very unique spatially varying uv fluorescence properties. The web uv images have colors significantly different from versions of them published in 1981. This paper examines whether the color difference indicates that the web images have deteriorated over time and if so whether information content in them is suspect. The limitations of the methodology used are discussed in the Introduction. Subject to these limitations, it is shown that deterioration probably has not occurred and that significant information can be extracted through image processing of the uv web images.
Analysis and improvement of non-contact SpO2 extraction using an RGB webcam
Authors: Wei Bing , Wu Xiaopei , Zhang Chao , Lv Zhao
Published: June 2021

Peripheral oxygen saturation (SpO2), a vital physiological sign employed in clinical care, is commonly obtained by using a contact pulse oximeter. With the rapid popularization of ordinary red-green-blue (RGB) webcams embedded in devices such as smartphones or laptops, there are broad application prospects for exploring techniques for non-contact SpO2 extraction using RGB webcams. However, many issues remain to be solved in the traditional webcam-based SpO2 extraction methods, such as the inherent low signal-to-noise ratio (SNR) of alternating current (AC) components of RGB signals and the potential defects in using RGB signals combination for SpO2 extraction. In this study, we conducted an in-depth examination of the existing research on webcam-based SpO2 extraction techniques, analyzed the practical problems in using them, and explored new ideas to solve the problems. Rather than roughly using the standard deviations (SD) of AC components for calculations, we performed blind source separation for AC components, and then used the energy coefficients retained in the mixed matrix to replace the variables required in the algorithm. Moreover, steady data was selected to compensate for the potential defects in using RGB signals combination. Through these efforts, the anti-noise capability of the algorithm was significantly enhanced, and the related defects were compensated for. The experimental results indicated that the proposed method produced reliable SpO2 estimation that could potentially—with further research—be used in real applications.
A compact photonic resonator absorption microscope for point of care digital resolution nucleic acid molecular diagnostics
Authors: Ghosh Shreya , Li Nantao , Xiong Yanyu , Ju Young-Gu , Rathslag Michael P. , Onal Ege G. , Falkiewicz Erika , Kohli Manish , Cunningham Brian T.
Published: June 2021

Rapid, sensitive, and selective detection of nucleic acid biomarkers for health diagnostic applications becomes feasible for point of care scenarios when the detection instrument is inexpensive, simple, and robust. Here, we report the design, implementation, and characterization of a point of care instrument for photonic resonator absorption microscopy (PRAM) that takes advantage of resonant optical coupling between plasmonic gold nanoparticle tags and a photonic crystal (PC) surface. Matching the PC resonant wavelength to the gold nanoparticle’s surface plasmon wavelength generates localized and efficient quenching of the PC resonant reflection intensity, resulting in the ability to clearly detect and count individual gold nanoparticles when they are captured on the PC surface. Surface-captured nanoparticles are observed by illuminating the PC at normal incidence with polarized light from a low-intensity red LED, and recording of PC reflected intensity on an inexpensive CMOS image sensor. A contrast limited adaptive histogram equalization (CLAHE) image processing algorithm was applied to derive counts of captured nanoparticles. The instrument is utilized in the context of an activate capture + digital counting (AC + DC) assay for a specific miRNA sequence, using nucleic acid toehold probes applied to gold nano-urchin (AuNU) nanoparticles to achieve 160 aM detection limits in a 30 min. assay.
Broadband trifunctional metasurface and its application in a lens antenna
Authors: Li Ximing , Chen Jian , Xi Xiang , Li Xin , Cheng Qiang , Wu Rui-Xin
Published: June 2021

Multifunctional metasurfaces have attracted extensive attention due to their ability to achieve diversified wavefront controls in flat devices. To date, most designs through metasurface are confined to realize one or two functionalities. In this work, we implement a broadband trifunctional metasurface by using different meta-atoms of the same type. The meta-atoms can independently manipulate the amplitude and phase of transmitted waves and the phase of reflected waves in a wide frequency range. Thus, they help the metasurface achieving the functionalities of beam deflection, diffuse scattering, and beam focusing according to the polarization and the direction of incident waves. The metasurface is applied to a metalens antenna, which features broadband, low side-lobe, and stealth. The metalens antenna works at the frequency range 9.8 GHz to 11.6 GHz with gain over 25 dBi. Experiments verify the functions of the trifunctional metasurface and are in good agreement with the designs. Our approach provides a solid platform for high-efficiency wideband metadevices with diverse functionalities.
Generating high-power Lissajous structured modes and trochoidal vortex beams by an off-axis end-pumped Nd:YVO4 laser with astigmatic transformation
Authors: Tuan P. H. , Cheng K. T. , Cheng Y. Z.
Published: June 2021

High-power structured beams with the transverse morphologies as the Lissajous figures are generated by an Nd:YVO4 laser under two-dimensional off-axis pumping. By fine-tuning the cavity length in the neighborhood around the condition of longitudinal-transverse coupling, different cases of accidental degeneracies from the intracavity astigmatism are achieved to lead the output emission to be various Lissajous modes with different transverse frequency ratios. The generated Lissajous modes reveals good power performance with slope efficiency up to 47% and optical-to-optical conversion efficiency to be higher than 37.5% at a pump power of 16 W. Moreover, by applying beam transformation via a single-lens astigmatic mode converter, the generated Lissajous modes are further converted into structured vortex beams with transverse patterns localized on the trochoidal curves. The transformed trochoidal vortex beams are confirmed to preserve well-defined mode structures even when the average output power has been scaled up to be higher than 4 W.
Conversion efficiency of soliton Kerr combs
Authors: Jang Jae K. , Okawachi Yoshitomo , Zhao Yun , Ji Xingchen , Joshi Chaitanya , Lipson Michal , Gaeta Alexander L.
Published: June 2021

We investigate the conversion efficiency (CE) of soliton modelocked Kerr frequency combs. Our analysis reveals three distinct scaling regimes of CE with the cavity free spectral range (FSR), which depends on the relative contributions of the coupling and propagation loss to the total cavity loss. Our measurements, for the case of critical coupling, verify our theoretical prediction over a range of FSRs and pump powers. Our numerical simulations also indicate that mode crossings have an adverse effect on the achievable CE. Our results indicate that microresonator combs operating with spacings in the electronically detectable regime are highly inefficient, which could have implications for integrated Kerr comb devices.
Machine learning enabled self-calibration single fiber endoscopic imaging
Authors: Zhang Huiying , Wang Xu , Du Hanwen , Yu Haiyang , Wu Jinghao , Meng Yanlong , Qiu Yanqing , Mao Bangning , Zhou Pengwei , Li Yi
Published: June 2021

Single fiber scanners (SFSs), with the advantages of compact size, versatility, large field of view, and high resolution, have been applied in many areas. However, image distortions persistently impair the imaging quality of the SFS, although many efforts have been made to address the problem. In this Letter, we propose a simple and complete solution by combining the piezoelectric (PZT) self-induction sensor and machine learning algorithms. The PZT tube was utilized as both the actuator and the fiber position sensor. Additionally, the feedback sensor signal was interrogated by a convolution neural network to eliminate the noise. The experimental results show that the predicted fiber trajectory error was below 0.1%. Moreover, this self-calibration SFS has an excellent robustness to temperature changes (20–50°C). It is believed that the proposed solution has removed the biggest barrier for the SFS and greatly improved its performance and stability in complex environments.
Sleep drive reconfigures wake-promoting clock circuitry to regulate adaptive behavior
Authors: Klose Markus K. , Shaw Paul J.
Published: June 2021

Circadian rhythms help animals synchronize motivated behaviors to match environmental demands. Recent evidence indicates that clock neurons influence the timing of behavior by differentially altering the activity of a distributed network of downstream neurons. Downstream circuits can be remodeled by Hebbian plasticity, synaptic scaling, and, under some circumstances, activity-dependent addition of cell surface receptors; the role of this receptor respecification phenomena is not well studied. We demonstrate that high sleep pressure quickly reprograms the wake-promoting large ventrolateral clock neurons to express the pigment dispersing factor receptor (PDFR). The addition of this signaling input into the circuit is associated with increased waking and early mating success. The respecification of PDFR in both young and adult large ventrolateral neurons requires 2 dopamine (DA) receptors and activation of the transcriptional regulator nejire (cAMP response element-binding protein [CREBBP]). These data identify receptor respecification as an important mechanism to sculpt circuit function to match sleep levels with demand.
Paradoxical phase response of gamma rhythms facilitates their entrainment in heterogeneous networks
Authors: Xu Xize , Riecke Hermann
Published: June 2021

The synchronization of different γ-rhythms arising in different brain areas has been implicated in various cognitive functions. Here, we focus on the effect of the ubiquitous neuronal heterogeneity on the synchronization of ING (interneuronal network gamma) and PING (pyramidal-interneuronal network gamma) rhythms. The synchronization properties of rhythms depends on the response of their collective phase to external input. We therefore determine the macroscopic phase-response curve for finite-amplitude perturbations (fmPRC) of ING- and PING-rhythms in all-to-all coupled networks comprised of linear (IF) or quadratic (QIF) integrate-and-fire neurons. For the QIF networks we complement the direct simulations with the adjoint method to determine the infinitesimal macroscopic PRC (imPRC) within the exact mean-field theory. We show that the intrinsic neuronal heterogeneity can qualitatively modify the fmPRC and the imPRC. Both PRCs can be biphasic and change sign (type II), even though the phase-response curve for the individual neurons is strictly non-negative (type I). Thus, for ING rhythms, say, external inhibition to the inhibitory cells can, in fact, advance the collective oscillation of the network, even though the same inhibition would lead to a delay when applied to uncoupled neurons. This paradoxical advance arises when the external inhibition modifies the internal dynamics of the network by reducing the number of spikes of inhibitory neurons; the advance resulting from this disinhibition outweighs the immediate delay caused by the external inhibition. These results explain how intrinsic heterogeneity allows ING- and PING-rhythms to become synchronized with a periodic forcing or another rhythm for a wider range in the mismatch of their frequencies. Our results identify a potential function of neuronal heterogeneity in the synchronization of coupled γ-rhythms, which may play a role in neural information transfer via communication through coherence.
Clinical and epidemiologic characteristics associated with dengue fever in 2011–2016 in Bang Phae district, Ratchaburi province, Thailand
Authors: Lim Jacqueline Kyungah , Chanthavanich Pornthep , Limkittikul Kriengsak , Lee Jung-Seok , Sirivichayakul Chukiat , Lee Kang Sung , Lim Sl-Ki , Yoon In-Kyu , Hattasingh Weerawan
Published: June 2021

Background

Dengue is a major public health problem in Thailand, but data are often focused on certain dengue-endemic areas. Methods: To better understand dengue epidemiology and clinical characteristics in Thailand, a fever surveillance study was conducted among patients aged 1–55 years, who presented with non-localized febrile illness at Bang Phae Community Hospital in Ratchaburi province, Thailand from October 2011 to September 2016.

Results

Among 951 febrile episodes, 130 were dengue-confirmed. Individuals aged 10–14 years were mostly affected, followed by those 15–19 years-of-age, with about 15% of dengue-confirmed cases from adults 25 years and older. There were annual peaks of dengue occurrence between June-November. Most prevalent serotype in circulation was DENV-2 in 2012, DENV-3 in 2014, and DENV-4 & -3 in 2015. Among dengue cases, 65% were accurately detected using the dengue NS1 RDT. Detection rate was similar between secondary and primary dengue cases where 66% of secondary vs. 60% of primary dengue cases had positive results on the NS1 RDT. Among dengue cases, 66% were clinically diagnosed with suspected dengue or DHF, prior to lab confirmation. Dengue was positively associated with rash, headache, hematemesis and alterations to consciousness, when compared to non-dengue. Dengue patients were 10.6 times more likely to be hospitalized, compared to non-dengue cases. Among dengue cases, 95 were secondary and 35 were primary infections. There were 8 suspected DHF cases and all were identified to be secondary dengue. Secondary dengue cases were 3.5 times more likely to be hospitalized compared to primary dengue cases. Although the majority of our dengue-positive patients were secondary dengue cases, with few patients showing manifestations of DHF, our dengue cases were mostly mild disease. Even among children < 10 years-of-age, 61% had secondary infection and the rate of secondary infection increased with age.

Conclusion

While the majority of dengue-confirmed cases were children, almost three-quarters of dengue-confirmed cases in this study were secondary dengue. Our study results consistent with previous data from the country confirm the hyperendemic transmission of DENV in Thailand, even in the non-epidemic years. With various interventions becoming available for dengue prevention and control, including dengue vaccines, decision-making on future implementation strategies should be based on such burden of disease data.
Long-term outcomes of ivermectin-albendazole versus albendazole alone against soil-transmitted helminths: Results from randomized controlled trials in Lao PDR and Pemba Island, Tanzania
Authors: Keller Ladina , Welsche Sophie , Patel Chandni , Sayasone Somphou , Ali Said M. , Ame Shaali M. , Hattendorf Jan , Hürlimann Eveline , Keiser Jennifer
Published: June 2021

Background

Preventive chemotherapy is the cornerstone of soil-transmitted helminth (STH) control. Long-term outcomes and adequate treatment frequency of the recently recommended albendazole-ivermectin have not been studied to date.

Methodology/principal findings

Double-blind randomized controlled trials were conducted in Lao PDR, Pemba Island, Tanzania and Côte d’Ivoire between 2018 and 2020 to evaluate the efficacy and safety of ivermectin-albendazole versus albendazole-placebo in Trichuris trichiura-infected individuals aged 6 to 60. In the framework of this study, in Lao PDR 466 and 413 participants and on Pemba Island, 558 and 515 participants were followed-up six and 12 months post-treatment, respectively. From each participant at least one stool sample was processed for Kato-Katz diagnosis and cure rates (CRs), egg reduction rates (ERRs) and apparent reinfection rates were calculated. If found helminth-positive at six months, participants were re-treated according to their allocated treatment.

Long-term outcomes against T. trichiura based on CRs and ERRs of ivermectin-albendazole compared to albendazole were significantly higher at six months in Lao PDR (CR, 65.8 vs 13.4%, difference; 52.4; 95% CI 45.0–60.0; ERRs, 99.0 vs 79.6, difference 19.4; 95% CI 14.4–24.4) and Pemba Island (CR, 17.8 vs 1.4%, difference; 16.4; 95% CI 11.6–21.0; ERRs, 84.9 vs 21.2, difference 63.8; 95% CI 50.6–76.9) and also at 12 months in Lao PDR (CR, 74.0 vs 23.4%, difference; 50.6; 95% CI 42.6–61.0; ERRs, 99.6 vs 91.3, difference 8.3; 95% CI 5.7–10.8) and Pemba Island (CR, 19.5 vs 3.4%, difference; 16.1; 95% CI 10.7–21.5; ERRs, 92.9 vs 53.6, difference 39.3; 95% CI 31.2–47.4) respectively.

Apparent reinfection rates with T. trichiura were considerably higher on Pemba Island (100.0%, 95% CI, 29.2–100.0) than in Lao PDR (10.0%, 95% CI, 0.2–44.5) at 12 months post-treatment for participants treated with albendazole alone.

Conclusions/significance

The long-term outcomes against T. trichiura of ivermectin-albendazole are superior to albendazole in terms of CRs and ERRs and in reducing infection intensities. Our results will help to guide decisions on how to best use ivermectin-albendazole in the context of large-scale PC programs tailored to the local context to sustainably control STH infections.

Trial registration

ClinicalTrials.gov registered with clinicaltrials.gov, reference: NCT03527732, date assigned: 17 May 2018.
Light-induced changes in the suprachiasmatic nucleus transcriptome regulated by the ERK/MAPK pathway
Authors: Alzate-Correa Diego , Aten Sydney , Campbell Moray J. , Hoyt Kari R. , Obrietan Karl
Published: June 2021

The mammalian master circadian pacemaker within the suprachiasmatic nucleus (SCN) maintains tight entrainment to the 24 hr light/dark cycle via a sophisticated clock-gated rhythm in the responsiveness of the oscillator to light. A central event in this light entrainment process appears to be the rapid induction of gene expression via the ERK/MAPK pathway. Here, we used RNA array-based profiling in combination with pharmacological disruption methods to examine the contribution of ERK/MAPK signaling to light-evoked gene expression. Transient photic stimulation during the circadian night, but not during the circadian day, triggered marked changes in gene expression, with early-night light predominately leading to increased gene expression and late-night light predominately leading to gene downregulation. Functional analysis revealed that light-regulated genes are involved in a diversity of physiological processes, including DNA transcription, RNA translation, mRNA processing, synaptic plasticity and circadian timing. The disruption of MAPK signaling led to a marked reduction in light-evoked gene regulation during the early night (32/52 genes) and late night (190/191 genes); further, MAPK signaling was found to gate gene expression across the circadian cycle. Together, these experiments reveal potentially important insights into the transcriptional-based mechanisms by which the ERK/MAPK pathway regulates circadian clock timing and light-evoked clock entrainment.
Implications of power imbalance in antenatal care seeking among pregnant adolescents in rural Tanzania: A qualitative study
Authors: Mweteni Wemaeli , Kabirigi Julieth , Matovelo Dismas , Laisser Rose , Yohani Victoria , Shabani Girles , Shayo Prosper , Brenner Jennifer , Chaput Katie
Published: June 2021

Background

Adolescent girls (10–19 years) are at increased risk of morbidity and mortality from pregnancy and childbirth complications, compared with older mothers. Low and middle-income countries, including Tanzania, bear the largest proportion of adolescent perinatal deaths. Few adolescent girls in Tanzania access antenatal care at health facilities, the reasons for which are poorly understood.

Methods

We conducted a qualitative thematic analysis study of the experiences of pregnant adolescents with accessing antenatal care in Misungwi district, Tanzania. We recruited 22 pregnant or parenting adolescent girls using purposive sampling, and conducted in-depth interviews (IDIs) about antenatal care experiences. IDI data were triangulated with data from eight focus group discussions (FGDs) involving young fathers and elder men/women, and nine key informant interviews (KIIs) conducted with local health care providers. FGDs, KIIs and IDIs were transcribed verbatim in Swahili. Transcripts were then translated to English and analysed using emergent thematic analysis.

Results

Four main themes emerged: 1) Lack of maternal personal autonomy, 2) Stigma and judgment, 3) Vulnerability to violence and abuse, and 4) Knowledge about antenatal care, and highlighted the complex power imbalance that underlies barriers and facilitators to care access at the individual, family/interpersonal, community, and health-systems levels, faced by pregnant adolescents in rural Tanzania.

Conclusion

Adolescent antenatal care-seeking is compromised by a complex power imbalance that involves financial dependence, lack of choice, lack of personal autonomy in decision making, experiences of social stigma, judgement, violence and abuse. Multi-level interventions are needed to empower adolescent girls, and to address policies and social constructs that may act as barriers, thereby, potentially reducing maternal morbidity and mortality in Tanzania.
Ecological stoichiometric characteristics of soil-moss C, N, and P in restoration stages of karst rocky desertification
Authors: Meng Wenping , Dai Quanhou , Ren Qingqing , Tu Na , Leng Tingjiao
Published: June 2021

Rocky desertification is the most serious ecological disaster in karst areas. Comprehensive control of rocky desertification plays an important role in promoting the economic development of karst areas. Studying the stoichiometric characteristics of mosses and soil can provide a powerful reference for the ecological restoration and evaluation of ecosystems experiencing rocky desertification. Soil and mosses were collected from sites representing different stages of ecological restoration (bare rock, grassland, shrubland, and secondary forest), and the contents of carbon (C), nitrogen (N), and phosphorus (P) were detected for ecological stoichiometric analysis. The results indicate that in different restoration stages following karst rocky desertification, the contents of soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP) and the stoichiometric ratios in the shrub habitat are higher than those in the bare rock, grassland, and secondary forest habitats. However, the TP and available P contents were low at all stages (0.06 g/kg and 0.62 mg/kg, respectively). The N and P contents and stoichiometric ratios in the mosses showed no significant differences among the succession stages. The C contents in the mosses had a significant positive correlation with SOC and TN and TP content, and the P content had a significant positive correlation with the soil available P. However, there was a significant negative correlation between the C: N and C:P ratios of the bryophytes and soil C: N. In summary, during the process of natural restoration of karst rocky desertification areas, SOC and soil TN contents accumulate with each succession stage. Soil nutrients are higher in shrub habitats than in other succession stages. Mosses have a strong effect on improving soil nutrients in rocky desertification areas.
Anticholinergic burden: First comprehensive analysis using claims data shows large variation by age and sex
Authors: Reinold Jonas , Braitmaier Malte , Riedel Oliver , Haug Ulrike
Published: June 2021

Purpose

The cumulative effect of medication inhibiting acetylcholine activity—also known as anticholinergic burden (AB)—can lead to functional and cognitive decline, falls, and death. Given that studies on the population prevalence of AB are rare, we aimed to describe it in a large and unselected population sample.

Methods

Using the German Pharmacoepidemiological Research Database (GePaRD) with claims data from ~20% of the German population we analyzed outpatient drug dispensations in 2016. Based on the Anticholinergic Cognitive Burden (ACB) scale, we classified persons into four categories and determined the cumulative AB as continuous variable.

Results

Among 16,470,946 persons (54% female), the prevalence of clinically relevant AB (ACB≥3) was 10% (women) and 7% (men). Below age 40 it was highest in persons ≤18 years (6% both sexes). At older ages (50–59 vs. 90–99 years), prevalence of ACB≥3 increased from 7% to 26% (men) and from 10% to 32% (women). Medication classes contributing to the cumulative AB differed by age: antihistamines, antibiotics, glucocorticoids (≤19 years), antidepressants (20–49 years), antidepressants, cardiovascular medication, antidiabetics (50–64 years), and additionally medication for urinary incontinence/overactive bladder (≥65 years). Medication dispensed by general physicians contributed most to the cumulative AB.

Conclusion

Although a clinically relevant AB is particularly common in older persons, prevalence in younger age groups was up to 7%. Given the risks associated with AB in older persons, targeted interventions at the prescriber level are needed. Furthermore, risks associated with AB in younger persons should be explored.
Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil
Authors: Younis Uzma , Rahi Ashfaq Ahmad , Danish Subhan , Ali Muhammad Arif , Ahmed Niaz , Datta Rahul , Fahad Shah , Holatko Jiri , Hammerschmiedt Tereza , Brtnicky Martin , Zarei Tayebeh , Baazeem Alaa , Sabagh Ayman EL , Glick Bernard R.
Published: June 2021

Fourier transform infrared spectroscopy (FTIR) spectroscopy detects functional groups such as vibrational bands like N-H, O-H, C-H, C = O (ester, amine, ketone, aldehyde), C = C, C = N (vibrational modes of a tetrapyrrole ring) and simply C = N. The FTIR of these bands is fundamental to the investigation of the effect of biochar (BC) treatment on structural changes in the chlorophyll molecules of both plants that were tested. For this, dried leaf of Spinacia oleracia (spinach) and Trigonella corniculata (fenugreek) were selected for FTIR spectral study of chlorophyll associated functional groups. The study’s primary goal was to investigate the silent features of infrared (IR) spectra of dried leave samples. The data obtained from the current study also shows that leaf chlorophyll can mask or suppress other molecules’ FITR bands, including proteins. In addition, the C = O bands with Mg and the C9 ketonic group of chlorophyll are observed as peaks at1600 (0%BC), 1650 (3%BC) and 1640, or near to1700 (5%BC) in spinach samples. In fenugreek, additional effects are observed in the FTIR spectra of chlorophyll at the major groups of C = C, C = O and C9 of the ketonic groups, and the vibrational bands are more evident at C-H and N-H of the tetrapyrrole ring. It is concluded that C-N bands are more visible in 5% BC treated spinach and fenugreek than in all other treatments. These types of spectra are useful in detecting changes or visibility of functional groups, which are very helpful in supporting biochemical data such as an increase in protein can be detected by more visibility of C-N bands in FTIR spectra.
A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station
Authors: Stahl-Rommel Sarah , Li David , Sung Michelle , Li Rebecca , Vijayakumar Aarthi , Atabay Kutay Deniz , Bushkin G. Guy , Castro Christian L. , Foley Kevin D. , Copeland D. Scott , Castro-Wallace Sarah L. , Alvarez Saavedra Ezequiel , Gleason Emily J. , Kraves Sebastian
Published: June 2021

As we explore beyond Earth, astronauts may be at risk for harmful DNA damage caused by ionizing radiation. Double-strand breaks are a type of DNA damage that can be repaired by two major cellular pathways: non-homologous end joining, during which insertions or deletions may be added at the break site, and homologous recombination, in which the DNA sequence often remains unchanged. Previous work suggests that space conditions may impact the choice of DNA repair pathway, potentially compounding the risks of increased radiation exposure during space travel. However, our understanding of this problem has been limited by technical and safety concerns, which have prevented integral study of the DNA repair process in space. The CRISPR/Cas9 gene editing system offers a model for the safe and targeted generation of double-strand breaks in eukaryotes. Here we describe a CRISPR-based assay for DNA break induction and assessment of double-strand break repair pathway choice entirely in space. As necessary steps in this process, we describe the first successful genetic transformation and CRISPR/Cas9 genome editing in space. These milestones represent a significant expansion of the molecular biology toolkit onboard the International Space Station.
Sports practice in Japan and France: A comparative analysis
Authors: Lefevre Brice , Nohara Hiroatsu , Nier Olivier
Published: June 2021

This article aimed to compare the sports participation of populations from two distinct societies with huge cultural differences: France and Japan. At a macro-statistical level and using a societal approach based on two national ad-hoc surveys–in contrast with functionalist and culturalist analyses–it analysed the differences in sports participation between the two populations. The angle adopted for analysing sports participation was that of voraciousness (number and types of sports and physical activities practised). We performed a secondary analysis of a Japanese national ad-hoc survey and a French national ad-hoc survey (quotas sampling, June/July 2016), which reported activities precisely over the preceding 12 months. The two reconstructed samples for comparison concerned people aged between 18 and 70, and 46 PSAs and PSA families, making it possible to evaluate sports participation. The Japanese sample consisted of 2,612 individuals and the French sample of 3,791 individuals. To identify statistically significant differences between the two samples, Fisher’s exact test and bootstrap approaches was used (boot package in R software). Sports participation and voraciousness for sport are higher in the French population than in the Japanese one. For the overall rate of sports practice, there is a difference of 8.2 points (95%CI[6.0;10.3], p<0.001), and the difference concerning the average number of activities practised by practitioners is 1.7 activity (95%CI[1.9;1.5], p<0.001). In both countries, young males have the highest level of sportiness. Age is an important variable with a lower difference between the youngest and oldest age groups for sports participation in Japan than in France: when compared to France, the difference in difference for the rate of no activity is 13.7 points (95%CI[6.3;21.1], p<0.001) and -2.3 activities for the average number of activities among practitioners (95%CI[-3.0;-1.7], p<0.001). Some physical and sports activities (PSAs) are specific to France (e.g. skiing with 12.7 points difference, 95%CI[11.3;14.1], p<0.001) and others to Japan, such as baseball (with 9.8 points difference 95%CI[8.6;11.0], p<0.001) or more traditional PSAs like Japanese calisthenics and radio exercise (at 15.9%). In contrast to France, Japan is still in the process of greatly modernising its sporting tradition as a result of its particular cultural dimensions. We can identify physical and sports activities which are specific to each country, as well as similar activities in the two countries and wider diversification in France. Voraciousness for sport is higher in France than in Japan. In both countries, the youngest age groups and men have the highest level of sportiness. Yet, the difference between the youngest and the oldest group is smaller in Japan due to the pressure of work.
Joint representation of color and form in convolutional neural networks: A stimulus-rich network perspective
Authors: Taylor JohnMark , Xu Yaoda
Published: June 2021

To interact with real-world objects, any effective visual system must jointly code the unique features defining each object. Despite decades of neuroscience research, we still lack a firm grasp on how the primate brain binds visual features. Here we apply a novel network-based stimulus-rich representational similarity approach to study color and form binding in five convolutional neural networks (CNNs) with varying architecture, depth, and presence/absence of recurrent processing. All CNNs showed near-orthogonal color and form processing in early layers, but increasingly interactive feature coding in higher layers, with this effect being much stronger for networks trained for object classification than untrained networks. These results characterize for the first time how multiple basic visual features are coded together in CNNs. The approach developed here can be easily implemented to characterize whether a similar coding scheme may serve as a viable solution to the binding problem in the primate brain.
Impact of the COVID-19 pandemic on biopsychosocial health and quality of life among Danish children and adults with neuromuscular diseases (NMD)—Patient reported outcomes from a national survey
Authors: Handberg Charlotte , Werlauff Ulla , Højberg Ann-Lisbeth , Knudsen Lone F.
Published: June 2021

The purpose was to investigate the impact of the COVID-19 pandemic on biopsychosocial health, daily activities, and quality of life among children and adults with neuromuscular diseases, and to assess the prevalence of COVID-19 infection and the impact of this in patients with neuromuscular diseases. The study was a national questionnaire survey. Responses were obtained from 811 adults (29%) and 67 parents of children (27%) with neuromuscular diseases. Many patients reported decreased health or physical functioning, and changes in access to physiotherapy or healthcare due to the pandemic. Participants generally perceived themselves or their child to be at high risk of severe illness from COVID-19, but only 15 patients had suffered from COVID-19 and experienced mild flu-like symptoms. 25.3% of adults and 46.6% of parents experienced anxiety. 20.4% of adults and 27.6% of parents experienced symptoms of depression. In general, the pandemic contributed to anxiety, a depressed mood as well as to fewer leisure activities, less social contact, isolation from work/school and a reduced quality of life, in particular for patients who perceived themselves to be at high risk of severe illness. The results demonstrate that the pandemic has had a negative impact on biopsychosocial health and quality of life of patients with neuromuscular diseases.
Determinants of maternal high-risk fertility behaviors and its correlation with child stunting and anemia in the East Africa region: A pooled analysis of nine East African countries
Authors: Tamirat Koku Sisay , Tesema Getayeneh Antehunegn , Tessema Zemenu Tadesse
Published: June 2021

Background

In low-income nations, high-risk fertility behavior is a prevalent public health concern that can be ascribed to unmet family planning needs, child marriage, and a weak health system. As a result, this study aimed to determine the factors that influence high-risk fertility behavior and its impact on child stunting and anemia.

Method

This study relied on secondary data sources from recent demography and health surveys of nine east African countries. Relevant data were extracted from Kids Record (KR) files and appended for the final analysis; 31,873 mother-child pairs were included in the final analysis. The mixed-effect logistic regression model (fixed and random effects) was used to describe the determinants of high-risk fertility behavior (HRFB) and its correlation with child stunting and anemia.

Result

According to the pooled study about 57.6% (95% CI: 57.7 to 58.2) of women had at least one high-risk fertility behavior, with major disparities found across countries and women’s residences. Women who lived in rural areas, had healthcare access challenges, had a history of abortion, lived in better socio-economic conditions, and had antenatal care follow-up were more likely to engage in high-risk fertility practices. Consequently, Young maternal age at first birth (<18), narrow birth intervals, and high birth orders were HRFBs associated with an increased occurrences of child stunting and anemia.

Conclusion

This study revealed that the magnitude of high-risk fertility behavior was higher in east Africa region. The finding of this study underscores that interventions focused on health education and behavioral change of women, and improvement of maternal healthcare access would be helpful to avert risky fertility behaviors. In brief, encouraging contraceptive utilization and creating awareness about birth spacing among reproductive-age women would be more helpful. Meanwhile, frequent nutritional screening and early intervention of children born from women who had high-risk fertility characteristics are mandatory to reduce the burden of chronic malnutrition.
Characterizing the social media footprint of general surgery residency programs
Authors: White Erin M. , Rohde Stefanie C. , Ruzgar Nensi M. , Chan Shin Mei , Esposito Andrew C. , Oliveira Kristin D. , Yoo Peter S.
Published: June 2021

Background

The medical community has increasingly embraced social media for a variety of purposes, including trainee education, research dissemination, professional networking, and recruitment of trainees and faculty. Platform choice and usage patterns appear to vary by specialty and purpose, but few studies comprehensively assess programs’ social media presence. Prior studies assessed general surgery departments’ Twitter use but omitted additional social media platforms and residency-specific accounts.

Objective

This study sought to broadly characterize the social media footprint of U.S. general surgery residency programs.

Methods

Using a protocolized search of program websites, social media platforms (Twitter, Facebook, Instagram, LinkedIn), and internet search, cross-sectional data on social media usage in March 2020 were collected for programs, their affiliated departments, their program directors (PDs), and their assistant/associate PDs (APDs).

Results

318 general surgery residency programs, 313 PDs, and 296 APDs were identified. 47.2% of programs had surgery-specific accounts on ≥1 platform. 40.2% of PDs and APDs had ≥1 account on Twitter and/or LinkedIn. Program type was associated with social media adoption and Twitter utilization, with lower usage among university-affiliated and independent programs (p<0.01).

Conclusions

Most general surgery residencies, especially non-university-based programs, lacked any department or residency accounts across Twitter, Facebook, and Instagram by March 2020. These findings highlight opportunities for increased social media engagement and act as a pre-pandemic baseline for future investigations of how the shift to virtual trainee education, recruitment, conferences, and clinical care affect social media use.
Exploring the multi-dimensional coordination relationship between population urbanization and land urbanization based on the MDCE model: A case study of the Yangtze River Economic Belt, China
Authors: Shan Ling , Jiang Yuehua , Liu Cuicui , Wang Yufei , Zhang Guanghong , Cui Xufeng , Li Fei
Published: June 2021

The rapid development of urbanization has had a dramatic impact on the economy, society and environment in China. In this context, the coordination relationship between population urbanization and land urbanization is essential for achieving sustainable urbanization. Based on the statistical data from 2007–2017 in the Yangtze River Economic Belt (YEB), this paper established the multi-dimensional coordination evaluation (MDCE) model by using the speed coordination evaluation (SCE) model, the level consistency evaluation (LCE) model, the entropy method and the space matching evaluation (SME) model to evaluate the coordination relationship between population urbanization and land urbanization from the speed-level-space perspective. The results showed that from 2007 to 2017: 1) the development speed of population urbanization and land urbanization in the YEB were more and more coordinated, and the speed of population urbanization lagged behind that of land urbanization. In addition, the overall development speed of the 11 provinces declined, and most of them were characterized by excessive development of land urbanization. 2) the development level of population urbanization and land urbanization in the YEB were all high, but the development level of population urbanization was lower than that of land urbanization. Further, the development level of the 11 provinces remained stable and high, and continuously improved. 3) the space matching of population urbanization and land urbanization in the YEB had a high degree of coordination, and the space matching degree of population urbanization was higher than that of land urbanization. Moreover, the space matching of most provinces in the region had declined, but the change was small. Finally, this paper proposes the policy recommendations on the coordinated development of population and land urbanization at the institutional, market and management levels to achieve coordinated and sustainable urbanization.
Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model
Authors: Fernández Javier , del Valle Fernández Iván , Villar Claudio J. , Lombó Felipe
Published: June 2021

In order to develop a fast combined method for onychomycosis treatment using an in vitro and an ex vivo models, a combination of two dual-diode lasers at 405 nm and 639 nm wavelengths, in a continuous manner, together with different ozone concentrations (until 80 ppm), was used for performing the experiments on fungal strains growing on PDA agar medium or on pig’s hooves samples. In the in vitro model experiments, with 30 min combined treatment, all species are inhibited at 40 ppm ozone concentration, except S. brevicaulis, which didn’t show an inhibition in comparison with only ozone treatment. In the ex vivo model experiments, with the same duration and ozone concentration, A. chrysogenum and E. floccosum showed total inhibition; T. mentagrophytes and T. rubrum showed a 75% growth inhibition; M. canis showed a delay in sporulation; and S. brevicaulis and A. terreus did not show growth inhibition. This combined laser and ozone treatment may be developed as a fast therapy for human onychomycosis, as a potential alternative to the use of antifungal drugs with potential side effects and long duration treatments.
Análise da expansão urbana na Zona Sul de Ilhéus e as condições de balneabilidade das praias:
Authors: Sampaio Sarah Andrade , Dos Santos Thiago Coelho , Silva Elisângela Rosemeri Martins
Published: June 2021

A cidade de Ilhéus vem passando por um processo de urbanização acelerada nos últimos anos, e hoje notadamente em destaque a zona sul, o que pode tornar as áreas de praias mais frágeis e vulneráveis ambientalmente. Neste contexto, a questão da alteração da paisagem e, as implicações advindas dessas interferências são importantes, visto que a cidade assenta-se na planície costeira, ambiente naturalmente frágil que denota restrições de uso. E, assim, evidencia-se também a necessidade de estudos voltados a balneabilidade das praias, principalmente por envolver questões de saúde pública e, por vezes, ambientais e econômicas, principalmente, voltadas ao turismo. O objetivo desta pesquisa consistiu em analisar as fragilidades e potencialidades da zona Sul na cidade de Ilhéus-BA. Nesta perspectiva, buscaram-se entender a acelerada urbanização desta área, sobretudo, nas últimas duas décadas e as alterações advindas de diferentes usos do solo, cujas características físicas também diferem, assim como, as condições de balneabilidade das praias. Tais preocupações se ampliam à medida que Ilhéus tenta se firmar como uma cidade turística, cuja beleza cênica na zona sul, por exemplo, fomenta essa expectativa, que, no entanto, na falta de um adequado planejamento, o turismo pode vir somados à impactos nocivos ao meio, até mesmo de maneira a inviabilizar tais atividades.
Structurally Unsound - Exploring Inequalities: Igniting research to better inform UK policy
Authors: Morris S , Patel O , Stainthorp C , Stevenson O
Published: June 2021

Inequalities are deeply embedded in our society, permeating throughout our social structures and institutions. Legislative responses that outlaw discriminatory behaviours and promote positive change are an essential part of the battle, but the structural nature of horizontal inequalities (that is, those that apply to entire groups such as women, disabled people, LGBT individuals, and people of colour rather than just at the individual level) mean that they are not necessarily sufficient. That is particularly the case once we account for additional complications associated with the intersection of various forms of horizontal inequality. The inequalities faced by women of colour are not simply those faced by white women with a racial element ‘added on’: they are fundamentally different. Making further progress rests, as ever, on securing political and social will for change. But it rests too on further developing the evidence base – both in terms of more accurately capturing the nuance of the problem statement, and better understanding what works when it comes to policy interventions. It is that goal which this project has pursued. Over the course of nine months, UCL and the Resolution Foundation have convened a series of roundtables and undertaken interviews with research and policy experts from a range of disciplines, policy areas, sectors and locations. Five cross‑cutting themes have emerged that we believe warrant consideration by all members of the research and policymaking communities that want to more effectively tackle structural inequalities in the UK: * Language / * Opportunity / * Understanding evidence / * Voice / * Place / We construct a deliberately technocratic list of lessons that researchers and policymakers should consider when thinking about how to better approach the study and treatment of structural inequalities. In this way, we hope to spread best practice and help plug the gaps in understanding that our expert engagement identified.