bluuebunny/crossref_metadata_2025 huggingface dataset:
A Grounded Theory of Interdisciplinary Communication and Collaboration in the Outpatient Setting of the Hospital for Patients with Multiple Long-Term Conditions
Authors: Gans Emma A. , de Ruijter Ursula W. , van der Heide Agnes , van der Meijden Suzanne A. , van den Bos Frederiek , van Munster Barbara C. , de Groot Janke F.
Published: May 2024

Interdisciplinary communication and collaboration are crucial in the care of people with multiple long-term conditions (MLTCs) yet are often experienced as insufficient. Through the lens of complexity science, this study aims to explain how healthcare professionals (HCPs) adapt to emerging situations in the care of patients with MLTC by examining interdisciplinary communication and collaboration in the outpatient hospital setting. We used the constant comparative method to analyze transcribed data from seven focus groups with twenty-one HCPs to generate a constructivist grounded theory of ‘interdisciplinary communication and collaboration in the outpatient setting of the hospital for patients with multiple long-term conditions’. Our theory elucidates the various pathways of communication and collaboration. Why, when, and how team members choose to collaborate influences if and to what degree tailored care is achieved. There is great variability and unpredictability to this process due to internalized rules, such as beliefs on the appropriateness to deviate from guidelines, and the presence of an interprofessional identity. We identified organizational structures that influence the dynamics of the care team such as the availability of time and financial compensation for collaboration. As we strive for tailored care for patients with MLTC, our theory provides promising avenues for future endeavors.
Risk-Based Gastrointestinal Parasite Control in a Tropical Zoological Institute
Authors: Heng Yirui , Chua Delia Hwee Hoon
Published: May 2024

The surveillance and treatment of parasites are important features of preventative healthcare plans in zoological institutes. The parasite control strategies employed in temperate regions often involve prophylactic anthelmintic treatments during seasons where the burden of gastrointestinal parasites in fecal testing is high. These strategies are, however, not applicable in the tropics, where temperatures remain high throughout the year, allowing continuous parasitic development. A risk-based parasite management strategy was adopted by a tropical zoological institute. For parasite surveillance, routine fecal direct smears and magnesium sulfate flotations were performed to determine parasitic prevalence. The frequency of fecal checks for the year was determined by the frequency at which clinically relevant parasitism (fecal tests that resulted in the animal being treated) was detected during routine fecal checks in the previous year. A yearly anthelmintic drug-class rotation schedule was also implemented. The total number of fecal tests performed per year and the number of animals with clinically significant parasitic disease decreased by 30.0% (637/2126) and 46.9% (207/451), respectively, over the four-year period of the study. Anthelmintic class rotation also improved the efficacy of fenbendazole in treating Strongyloides spp. infecting the group of orangutans. This parasite control strategy is a targeted approach to managing preventative healthcare, reducing the work required to perform routine surveillance tests whilst maintaining the health of the collection of animals.
Assessment Methodology for Physical Vulnerability of Vernacular Architecture in Areas Affected by Depopulation: The Case of Comunidad Valenciana, Spain
Authors: Tortajada Montalvá Eva , Mileto Camilla , Vegas López-Manzanares Fernando
Published: May 2024

The intensity with which the phenomenon of depopulation has affected rural municipalities in Spain between 1950 and 2022 has led to a loss in the intergenerational transmission of traditional knowledge, values and customs. Sociocultural loss entails associated physical risks: the abandonment, demolition, and loss of vernacular architecture. This research analyzes the evolution of this type of architecture in a period of acute depopulation and its current state of conservation. A total of 180 case studies in the region of Comunidad Valenciana are analyzed through four factors affecting the physical vulnerability of dwellings: year of construction, state of conservation, type of use, and a combination of all three. Data management software is used to combine all the information and produce the results in a tabular and graphical format, while the Geographic Information System is used to draw up risk maps showing the results. These results are then divided into analysis groups created according to the degree of depopulation observed in the years mentioned. This made it possible to identify the relationship between depopulation and the conservation of vernacular architecture, showing the risk level for each case study, and thus creating an analysis methodology applicable in other territories affected by depopulation at a national and international level.
Research on Post-Use Evaluation of Community Green Space Rectification Based on a Multi-Dimensional Perception System: A Case Study of Jiayuan Sanli Community in Beijing
Authors: Li Meng , Zhang Jian , Wang Yuchen
Published: May 2024

Community green spaces (CGSs) constitute a crucial element of urban land use, playing a pivotal role in maintaining the stability of urban ecosystems and enhancing the overall quality of the urban environment. Through the post-occupancy evaluation (POE) of green spaces, we can gain insights into residents’ actual needs and usage habits, providing scientific evidence for the planning, design, and management of green spaces. This ensures that CGSs better meet residents’ needs and improve their quality of life. The POE of CGSs relies heavily on high-precision data support. However, the current POE system for CGSs faces challenges, such as limited data collection methods, incomplete indicator systems, and excessive manual involvement. To address these limitations in data collection, this study proposes a comprehensive, dynamically monitored, objective, and sustainable POE system for CGSs. This system incorporates a multi-dimensional perception system that integrates the Internet of Things (IoT) and sensors to collect data from various sources. It establishes an evaluation framework from the perspectives of policy guidance and usage needs for CGSs, utilizing neural network systems and artificial intelligence techniques to compute the evaluation results. Using the Jiayuan Sanli Community in Beijing as a case study, this paper demonstrates the feasibility of the proposed system. A comparison between the POE results obtained using the multi-dimensional perception technique and those obtained manually reveals an 87% improvement in the accuracy of the evaluation results based on the multi-dimensional perception system. This system bridges the gap between planning perspectives and user experiences, contributing significantly to future urban land planning and land policy formulation.
Biotope Map Creation Method and Utilization Plan for Eco-Friendly Urban Development
Authors: Kim Taehun , Kim Gunwoo
Published: May 2024

A biotope map provides ecological and spatial information that reflects the natural and ecological features and values of a city. In South Korea, efforts have been made to create a map that includes grades based on evaluating the ecological value of a specific surface space. However, plans for applications have not been established, except for development restrictions or regulations for biotopes with high ecological value ratings. The aim of this study was to promote environmentally friendly and sustainable urban management through ecological land use management for all biotope types that are influenced by anthropogenic land use. Strategies for maintenance, protection, recovery, improvement, creation, and reduction based on ecological restoration principles were set as management goals. To achieve these management goals, evaluation items and indicators were suggested for minor-classification types and applied to Dongducheon City. Management strategies were suggested for target sites based on grades and thematic maps of biotope types to be utilized, such as urban ecological axes, river naturality restoration, and wetland and forest preservation. These findings support sustainable and environmentally friendly urban development by providing fundamental data for ecological and environmental management, including the preservation and restoration of natural environments and the creation of urban ecological networks.
Historical Insights into Sustainable Development: Analyzing the Spatiotemporal Dynamics of Ancient Trade and Settlements
Authors: Zeng Zhanjing , Shen Caiyun , Xu Minghui
Published: May 2024

Ancient trade routes significantly influenced political structures, cultural exchanges, economic development, and landscape transformations across diverse regions. The Ancient Tea Horse Road, a prominent historical commercial pathway in China, is crucial for ecosystem and cultural heritage conservations. This study uses content and comparative analysis, Geographic Information System (GIS) technology, and spatial analysis to examine the spatiotemporal relationships between 13,026 settlement points identified via Google Earth and 445 recorded settlement names within the Dali Bai Autonomous Prefecture, Yunnan Province. The findings indicate that the Ancient Tea Horse Road evolved from a simple linear form to a complex radial network, reflecting its growth from local to broad regional connectivity. Settlements related to administrative, postal, and commercial functions emerged in its vicinity, underscoring the route’s impact on social structures and economic activities. The development of settlements, mainly within a 10 km radius of the route, was closely linked to its expansion, alteration, and decline, offering insights into contemporary settlement distribution patterns. This research not only enhances the understanding of the Ancient Tea Horse Road and its surrounding settlements but also supports the protection of ancient trade routes and their cultural heritage. It contributes to sustainable development by integrating ecological preservation with the safeguarding of ancient trade routes and their cultural legacies.
Environmental Inequality in Peri-Urban Areas: A Case Study of Huangpu District, Guangzhou City
Authors: Shen Jing , Wang Shaogu , Wang Yuyin
Published: May 2024

This research investigates environmental inequalities within Guangzhou’s Huangpu District against the backdrop of rapid urbanisation and industrial expansion in Asia. This study identifies environmental hotspots, particularly in socially vulnerable areas characterised by high industrial density, using a vulnerability framework and analysing census and pollution data. Utilising satellite imagery, urban planning documents, and field research, we delve into the internal environmental conflicts arising from industrial land use. Our findings reveal how diverse stakeholders, guided by their rationales and interests, collectively contribute to spatial inequalities within a market-driven context. Importantly, we emphasise that environmental inequality transcends mere conflicts of interest among stakeholders and is fundamentally shaped by the prevailing market-oriented spatial development model in peri-urban areas. This model results in urban segmentation, socio-economic stratification, and an uneven distribution of environmental risks and resources. Our study advocates for a paradigmatic shift in China’s peri-urban spatial development and the integration of environmental protection and social equity alongside economic growth. We recommend moving away from short-term speculative practices and promoting long-term, community-engaged urban renewal strategies that harmonise economic progress with improved living standards and environmental sustainability.
Assessment of Phytomass and Carbon Stock in the Ecosystems of the Central Forest Steppe of the East European Plain: Integrated Approach of Terrestrial Environmental Monitoring and Remote Sensing with Unmanned Aerial Vehicles
Authors: Slavskiy Vasiliy , Matveev Sergey , Sheshnitsan Sergey , Litovchenko Daria , Larionov Maxim Viktorovich , Shokurov Anton , Litovchenko Pavel , Durmanov Nikolay
Published: May 2024

The rapid and accurate estimation of aboveground forest phytomass remains a challenging research task. In general, methods for estimating phytomass fall mainly into the category of field measurements performed by ground-based methods, but approaches based on remote sensing and ecological modelling have been increasingly applied. The aim is to develop the scientific and methodological framework for the remote sensing estimation of qualitative and quantitative characteristics of forest stands, using the combination of surveys and machine learning models to determine phytomass of forest stands and calculate the carbon balance. Even-aged stands of different tree species growing in the forest steppe zone of the East European Plain were chosen as test objects. We have applied the modernized methodological approaches to compare and integrate forest and tree stand characteristics obtained by ground-based and UAV-based comprehensive surveys; additionally, we developed computer vision models and methods for determining the same characteristics by remote sensing methods. The key advantage of the proposed methodology for remote monitoring and carbon balance control over existing analogues is the minimization of the amount of groundwork and, consequently, the reduction inlabor costs without loss of information quality. Reliable data on phytomass volumes will allow for operational control of the forest carbon storage, which is essential for decision-making processes. This is important for the environmental monitoring of forests and green spaces of various economic categories. The proposed methodology is necessary for the monitoring and control of ecological–climatic and anthropogenic–technogenic transformations in various landscapes. The development is useful for organizing the management of ecosystems, environmental protection, and managing the recreational and economic resources of landscapes with natural forests and forest plantations.
Comparison of the Effects of Pilates and Yoga Exercise on the Dynamic Balancing Ability and Functional Movement of Fencers
Authors: Lim So-Jung , Kim Hyun-Jin , Kim Yong-Soo , Kim Eunkuk , Hwang Inyoung , Kang Ju-Seop
Published: May 2024

This study was conducted to compare and analyze whether Pilates exercise and yoga exercise help improve the performance of female fencers and prevent injury, and the dynamic balance test (LQ-YBT) and functional movement screening (FMS) test score of the elite adult female fencers were compared and analyzed as evaluation indicators. Participants were randomly classified into Pilates (n = 10) and yoga groups (n = 10), members of which took part in 50 min of exercise (5 min of warm-up, 40 min of main exercise, and 5 min of cool-down) twice weekly for eight weeks. The results obtained from this study were analyzed via independent t-test and 2-way ANOVA. The results were as follows: LQ-YBT measures (reaching distance) increased significantly for both groups, as did FMS scores (deep squat, hurdle step, inline lunge, shoulder mobility, active straight-leg raise, trunk-stability push-up, and rotary stability). These results suggest that Pilates exercise and yoga exercise might be likely effective in improving the performance of adult female fencers and injury prevention by increasing their dynamic balance ability and functional movement.
Alterations in Blood–Brain Barrier Integrity and Lateral Ventricle Differ in Rats Exposed to Space Radiation and Social Isolation
Authors: Adkins Austin M. , Luyo Zachary N. M. , Gibbs Alayna J. , Boden Alea F. , Heerbrandt Riley S. , Gotthold Justin D. , Britten Richard A. , Wellman Laurie L. , Sanford Larry D.
Published: May 2024

The proposed Mars missions will expose astronauts to long durations of social isolation (SI) and space radiation (SR). These stressors have been shown to alter the brain’s macrostructure and microenvironment, including the blood–brain barrier (BBB). Breakdown of the BBB is linked to impaired executive functions and physical deficits, including sensorimotor and neurocognitive impairments. However, the precise mechanisms mediating these effects remain unknown. Additionally, the synergistic effects of combined exposure to SI and SR on the structural integrity of the BBB and brain remain unknown. We assessed the BBB integrity and morphology in the brains of male rats exposed to ground-based analogs of SI and SR. The rats exposed to SR had enlarged lateral ventricles and increased BBB damage associated with a loss of astrocytes and an increased number of leaky vessels. Many deficits observed in SR-treated animals were attenuated by dual exposure to SI (DFS). SI alone did not show BBB damage but did show differences in astrocyte morphology compared to the Controls. Thus, determining how single and combined inflight stressors modulate CNS structural integrity is crucial to fully understand the multiple pathways that could impact astronaut performance and health, including the alterations to the CNS structures and cell viability observed in this study.
Advances in Contact Mechanics
Authors: Liu Haichao , Zhang Haibo , Ding Xiaoyu
Published: May 2024

Advancements in contact mechanics play an important role in the design of modern mechanical and bio-medical systems, enhancing their efficiency, power density, and reliability [...]
Objective Evaluation of Motion Cueing Algorithms for Vehicle Driving Simulator Based on Criteria Importance through Intercriteria Correlation (CRITIC) Weight Method Combined with Gray Correlation Analysis
Authors: Jiang Xue , Chen Xiafei , Jiao Yiyang , Zhang Lijie
Published: May 2024

Perception-based fidelity evaluation metrics are crucial in driving simulators, as they play a key role in the automatic tuning, assessment, and comparison of motion cueing algorithms. Nevertheless, there is presently no unified and effective evaluation framework for these algorithms. To tackle this challenge, our study initially establishes a model rooted in visual–vestibular interaction and head tilt angle perception systems. We then employ metrics like the Normalized Average Absolute Difference (NAAD), Normalized Pearson Correlation (NPC), and Estimated Delay (ED) to devise an evaluation index system. Furthermore, we use a combined approach incorporating CRITIC and gray relational analysis to ascertain the weights of these indicators. This allows us to consolidate them into a comprehensive evaluation metric that reflects the overall fidelity of motion cueing algorithms. Subjective evaluation experiments validate the reasonableness and efficacy of our proposed Perception Fidelity Evaluation (PFE) method.
An Image-Based Interactive Training Method of an Upper Limb Rehabilitation Robot
Authors: Ye Changlong , Wang Zun , Yu Suyang , Jiang Chunying
Published: May 2024

Aimed at the problem of human–machine interaction between patients and robots in the process of using rehabilitation robots for rehabilitation training, this paper proposes a human–machine interactive control method based on an independently developed upper limb rehabilitation robot. In this method, the camera is used as a sensor, the human skeleton model is used to analyse the moving image, and the key points of the human body are extracted. Then, the three-dimensional coordinates of the key points of the human arm are extracted by depth estimation and spatial geometry, and then the real-time motion data are obtained, and the control instructions of the robot are generated from it to realise the real-time interactive control of the robot. This method can not only improve the adaptability of the system to individual patient differences, but also improve the robustness of the system, which is less affected by environmental changes. The experimental results show that this method can realise real-time control of the rehabilitation robot, and that the robot assists the patient to complete the action with high accuracy. The results show that this control method is effective and can be applied to the fields of robot control and robot-assisted rehabilitation training.
Feedback Stabilization of Quasi-One-Sided Lipschitz Nonlinear Discrete-Time Systems with Reduced-Order Observer
Authors: Zhao Yanbin , Dong Wenqiang
Published: May 2024

The feedback stabilization problem for nonlinear discrete-time systems with a reduced-order observer is investigated, in which the nonlinear terms of the systems satisfy the quasi-one-sided Lipschitz condition. First, a discrete-time reduced-order observer for nonlinear systems is designed. Then, a feedback controller with a reduced-order observer is designed for realizing the stabilization of nonlinear discrete-time systems. We prove that the design of a feedback controller and reduced-order observer of systems can be carried out independently in the case of discrete-time with nonlinear terms, which largely reduces the computational complexity of the observer and controller. The introduction of the quasi-one-sided Lipschitz condition simultaneously enhances the robustness and stability of nonlinear control systems. Finally, the feasibility and effectiveness of the proposed design approach is verified by a numerical simulation.
High ADMA Is Associated with Worse Health Profile in Heart Failure Patients Hospitalized for Episodes of Acute Decompensation
Authors: Vîlcea Anamaria , Borta Simona Maria , Popețiu Romana Olivia , Alexandra Rus Larisa , Pilat Luminița , Nica Dragoș Vasile , Pușchiță Maria
Published: May 2024

Background and Objectives: episodes of acute decompensation in chronic heart failure (ADHF), a common health problem for the growing elderly population, pose a significant socio-economic burden on the public health systems. Limited knowledge is available on both the endothelial function in and the cardio-metabolic health profile of old adults hospitalized due to ADHF. This study aimed to investigate the connection between asymmetric dimethylarginine (ADMA)—a potent inhibitor of nitric oxide—and key health biomarkers in this category of high-risk patients. Materials and Methods: this pilot study included 83 individuals with a known ADHF history who were admitted to the ICU due to acute cardiac decompensation. Selected cardiovascular, metabolic, haemogram, renal, and liver parameters were measured at admission to the ICU. Key renal function indicators (serum creatinine, sodium, and potassium) were determined again at discharge. These parameters were compared between patients stratified by median ADMA (114 ng/mL). Results: high ADMA patients showed a significantly higher incidence of ischemic cardiomyopathy and longer length of hospital stay compared to those with low ADMA subjects. These individuals exhibited significantly higher urea at admission and creatinine at discharge, indicating poorer renal function. Moreover, their lipid profile was less favorable, with significantly elevated levels of total cholesterol and HDL. However, no significant inter-group differences were observed for the other parameters measured. Conclusions: the present findings disclose multidimensional, adverse ADMA-related changes in the health risk profile of patients with chronic heart failure hospitalized due to recurrent decompensation episodes.
Fly-Cutting Processing of Micro-Triangular Pyramid Arrays and Synchronous Micro-Scrap Removal
Authors: Gao Jiashun , Xu Zhilong , Lei Yu , Huang Su
Published: May 2024

Many micro-scraps are generated when a micro-triangular pyramid array (MTPA) is machined by the fly-cutting method. Micro-scraps are generally not removed quickly enough; therefore, these residual micro-scraps participate in the cutting process again, scratching the workpiece surface and accelerating diamond tool wear. To remove micro-scraps rapidly, a fly-cutting method to produce MTPAs on vertically oriented working surfaces was developed during this study. The results show that an MTPA produced by fly cutting on a vertical workpiece had a clearly outlined structure, high dimensional accuracy, and a low surface roughness. There was no micro-scrap residue on the workpiece surface and the diamond tool wear was small. The cutting inlet edges had no burrs, and the cutting outlet edges had only a small number of burrs. This method of fly cutting MTPAs on vertically oriented working surfaces provides a foundation for the development of high-precision micro-triangular pyramid optical elements.
Exploring the Antimicrobial and Antioxidant Activities of Streptomyces sp. EIZ2 Isolated from Moroccan Agricultural Soil
Authors: Rammali Said , Kamal Fatima Zahra , El Aalaoui Mohamed , Rahim Abdellatif , Baidani Aziz , Dari Khadija , Khattabi Abdelkrim , Ciobică Alin , Novac Bogdan , Petroaie Antoneta , Lefter Radu , Bencharki Bouchaib
Published: May 2024

Antibiotics play a crucial role in preserving and improving public health, saving millions of lives every year. However, their effectiveness is currently under threat due to the ability of bacteria to adapt and develop resistance to these treatments. Therefore, this study was carried out on two soil samples collected in two areas of Arba Aounate, Sidi Bennour province, Morocco, to identify natural antibiotic-producing Actinobacteria capable of combating multi-drug-resistant (MDR) bacteria. A primary screening revealed that of the 50 isolates, 16 exhibited antimicrobial activity against Pseudomonas aeruginosa ATCC 27,853, Staphylococcus aureus ATCC 25,923, Escherichia coli ATCC 25,922, and Candida albicans ATTC 60193. A secondary screening showed that of the 16 isolates, only EIZ1 and EIZ2 isolates displayed outstanding antimicrobial and antifungal activity against 6 MDR bacteria, including Escherichia coli 19L2418, Listeria monocytogenes, Proteus sp. 19K1313, Klebsiella pneumoniae 19K 929, Proteus vulgaris 16C1737, and Klebsiella pneumoniae 20B1572. These two isolates were also characterized culturally, morphologically, physiologically, and biochemically. Afterward, the amplification of 16S rRNA revealed that isolate EIZ2 was 99.06% strongly related to the genus Streptomyces. Furthermore, this extract exhibits strong antioxidant activity against DPPH and ABTS free radicals and elevated ferric-reducing antioxidant power. A significant (p < 0.0001) positive correlation was observed between antioxidant activities and total phenolic and flavonoid contents. A GC–MS analysis of the ethyl acetate extract revealed the presence of 10 compounds, mainly diethyl phthalate (97%) and benzeneacetic acid (94%). This research demonstrates that Streptomyces sp. strain EIZ2 represents a potential source of antimicrobial and antioxidant compounds. These compounds could offer considerable potential as therapeutic agents, paving the way for future developments in medical applications.
Microbial Diversity and Nitrogen Cycling in Peat and Marine Soils: A Review
Authors: Soratur Akshatha , Venmathi Maran Balu Alagar , Kamarudin Ahmad Syazni , Rodrigues Kenneth Francis
Published: May 2024

Nitrogen is an essential nutrient for living organisms in peat and marine soils, and its transformation within the soil matrix is a complex process mediated by various microbes that inhabit these ecological niches. The metabolism of nitrogen is governed by microbially mediated biogeochemical transformations, such as nitrification, anammox, and denitrification, which contribute to the assimilated pool of nitrogen and fixed nitrogen loss. One of the major challenges facing the field of peat and marine microbiology is the lack of understanding of the correlation between ecosystem-driven nitrogen transformation and microbial diversity. This is crucial because of growing concerns regarding the impacts of human-induced activities and global climate change on microbial nitrogen-cycling processes in peat and marine soils. Thus, this review aimed to provide a comprehensive overview of the current understanding of the microbial communities involved in peat and marine nitrification, anammox, and denitrification; the factors influencing the niche differentiation and distribution of the main functional components; the genes involved; and the main effects of human-induced activities and global climate change on the peat and marine nitrogen cycle. The implications of this review will facilitate an understanding of the complex mechanisms associated with ecosystem function in relation to nitrogen cycling, the role of peat and marine soils as carbon sinks, pollution remediation using naturally occurring populations of diverse microbes, and the development of policies to mitigate the effects of anthropogenic influences in peat and marine soils.
Assessment of the Safety and Probiotic Properties of Enterococcus faecium B13 Isolated from Fermented Chili
Authors: Xiao Jingmin , Chen Cai , Fu Zhuxian , Wang Shumin , Luo Fan
Published: May 2024

Enterococcus faecium B13, selected from fermentation chili, has been proven to promote animal growth by previous studies, but it belongs to opportunistic pathogens, so a comprehensive evaluation of its probiotic properties and safety is necessary. In this study, the probiotic properties and safety of B13 were evaluated at the genetic and phenotype levels in vitro and then confirmed in vivo. The genome of B13 contains one chromosome and two plasmids. The average nucleotide identity indicated that B13 was most closely related to the fermentation-plant-derived strain. The strain does not carry the major virulence genes of the clinical E. faecium strains but contains aac(6′)-Ii, ant (6)-Ia, msrC genes. The strain had a higher tolerance to acid at pH 3.0, 4.0, and 0.3% bile salt and a 32.83% free radical DPPH clearance rate. It can adhere to Caco-2 cells and reduce the adhesion of E. coli to Caco-2 cells. The safety assessment revealed that the strain showed no hemolysis and did not exhibit gelatinase, ornithine decarboxylase, lysine decarboxylase, or tryptophanase activity. It was sensitive to twelve antibiotics but was resistant to erythromycin, rifampicin, tetracycline, doxycycline, and minocycline. Experiments in vivo have shown that B13 can be located in the ileum and colon and has no adverse effects on experiment animals. After 28 days of feeding, B13 did not remarkable change the α-diversity of the gut flora or increase the virulence genes. Our study demonstrated that E. faecium B13 may be used as a probiotic candidate.
Study of the Impact of the Parasitic Microalgae Coccomyxa parasitica on the Health of Bivalve Modiolus kurilensis
Authors: Tumas Ayna V. , Slatvinskaya Veronika A. , Kumeiko Vadim V. , Sokolnikova Yulia N.
Published: May 2024

The invasion of bivalves by parasitic microalgae Coccomyxa is widespread and causes pathologies and dysfunctions of the organs, especially in the most valuable products: the mantle and the muscle. The pathogenesis of the disease remains completely unknown. In this study, based on a macroscopic examination of Modiolus kurilensis and microalgae count in each infected individual, four stages of disease development with characteristic pathognomonic symptoms were described. During the progression of the disease, the concentration of alkaline phosphatase, glucose, calcium, hemolytic and agglutinating activities, number of basophils, eosinophils, phagocytes, and cells with reactive oxygen species increased in the hemolymph, while number of agranulocytes, cells with lysosomes, dead hemocytes, total protein concentration, as well as the weight of mollusks decreased. In the nephridia and digestive gland, necrosis, invasion of Nematopsis sp., hemocyte infiltration, and fibrosis increased. The ratio of changed tubules and occurrence of granulocytomas increased in the digestive gland, while the base membrane, nephrocytes and concretions changed in the nephridia. This study helps establish the variability of these parameters under normal conditions and their alteration during the disease. Moreover, these findings can be used for veterinary monitoring of the state of bivalves in natural and aquaculture populations.
Differential Epigenetic Regulation in Uninfected and Tuberculosis–Human Immunodeficiency Virus Co-Infected Patients
Authors: Mamabolo Katlego , Wadee Reubina , Perner Yvonne , Magangane Pumza , Duze Sanelisiwe Thinasonke , Marimani Musa
Published: May 2024

This study aimed to compare the degree of epigenetic modifications between a TB-HIV co-infected cohort and uninfected subjects. Formalin-fixed paraffin-embedded (FFPE) tissues were retrieved from 45 TB-HIV co-infected and 45 control individuals. Real-time PCR was applied to compare the level of expression of genes involved in epigenetic regulation. The protein multiplex assay was used to assess the degree of protein modification. DNA sequencing was used to determine the evolutionary relationships between the infecting HIV and Mtb strains. Our results indicated a significant increase in the expression of the five candidate genes in the patients with TB-HIV relative to the control cohort. A sharp increase in the degree of histone methylation, acetylation and phosphorylation was observed in TB-HIV co-infected patients. The phylogenetic analysis classified the strains into three distinct HIV clusters and five Mtb clusters. The disparities in the expression profiles of our candidate genes between the TB-HIV cohort and non-TB-HIV group highlights the important role played by various TB and HIV strains in regulating the host gene expression landscape.
Exploring the Toxin-Mediated Mechanisms in Clostridioides difficile Infection
Authors: Pourliotopoulou Evdokia , Karampatakis Theodoros , Kachrimanidou Melania
Published: May 2024

Clostridioides difficile infection (CDI) is the leading cause of nosocomial antibiotic-associated diarrhea, and colitis, with increasing incidence and healthcare costs. Its pathogenesis is primarily driven by toxins produced by the bacterium C. difficile, Toxin A (TcdA) and Toxin B (TcdB). Certain strains produce an additional toxin, the C. difficile transferase (CDT), which further enhances the virulence and pathogenicity of C. difficile. These toxins disrupt colonic epithelial barrier integrity, and induce inflammation and cellular damage, leading to CDI symptoms. Significant progress has been made in the past decade in elucidating the molecular mechanisms of TcdA, TcdB, and CDT, which provide insights into the management of CDI and the future development of novel treatment strategies based on anti-toxin therapies. While antibiotics are common treatments, high recurrence rates necessitate alternative therapies. Bezlotoxumab, targeting TcdB, is the only available anti-toxin, yet limitations persist, prompting ongoing research. This review highlights the current knowledge of the structure and mechanism of action of C. difficile toxins and their role in disease. By comprehensively describing the toxin-mediated mechanisms, this review provides insights for the future development of novel treatment strategies and the management of CDI.
Selective Recovery of Gold from E-Waste Recycling Plants’ Waste Fractions: Waste-to-Resource Transition
Authors: Zueva Svetlana , Ippolito Nicolò Maria , Passadoro Marco , Romano Pietro , Ferella Francesco , Vegliò Francesco
Published: May 2024

Electronic waste grows globally at a rate of 5% annually, which makes electronic waste recycling (WEEE recycling) an urgent task aimed at achieving (i) environmental protection and (ii) the preservation of mineral resources through the re-introduction of strategic metals into the market. As it turns out, WEEE recycling produces further waste, called WEEE secondary waste, which still contains valuable metals such as gold, silver, and copper. This study assessed the economic viability of recovering these metals and identified the most promising targets and approaches. WEEE secondary waste produced at a plant in southern Europe was sampled and examined for this purpose. The study methods included an ANOVA (analysis of variance) and an OCCP (orthogonal central composition plan). Over 90% gold, silver, and copper extraction was achieved with hydrochloric acid leaching in sodium hypochlorite with sodium chlorite as an oxidizing agent at 60 °C. The significance of the variation in the response for each factor, calculated using the Yates algorithm, demonstrates that by excluding sodium chloride and optimizing the doses of hydrochloric acid and sodium hypochlorite, gold can be selectively recovered through the leaching process. The scenario of HCl (2.5M)/NaClO (5%) leaching results in the maximum extraction effect of Au (91.6%) at the lowest concentrations of Ag and Cu (37–44%).
Decabromodifenyl Ether (BDE-209) in Surface Soils from Warsaw and Surrounding Areas: Characterization of Non-Carcinogenic Risk Associated with Oral and Dermal Exposure
Authors: Korcz Wojciech , Czaja Katarzyna , Liszewska Monika , Lewiński Radosław , Słomczyńska Anna , Struciński Paweł
Published: May 2024

Polybrominated diphenyl ethers (PBDEs) have been used for many years as flame retardants. Due to their physicochemical and toxicological properties, they are considered to be persistent organic pollutants (POPs). BDE-209 is the main component of deca-BDE, the one PBDE commercial mixture currently approved for use in the European Union. The aim of this study was to analyse BDE-209 in surface soil samples from Warsaw and surrounding areas (Poland) as an indicator of environmental pollution with PBDEs, and to characterise the associated health risk. A total of 40 samples were analysed using gas chromatography with electron capture detection (GC-µECD). Concentrations of BDE-209 in soil ranged from 0.4 ng g−1 d.w. (limit of quantification) to 158 ng g−1 d.w. Overall, 52.5% of results were above the method’s limit of quantification. The highest levels were found at several locations with heavy traffic and in the vicinity of a CHP plant in the city. The lowest concentrations were observed in most of the samples collected from low industrialized or green areas (<0.4 to 1.68 ng g−1 d.w.). Exposure to BDE-209 was estimated for one of the most sensitive populations, i.e., young children. The following exposure routes were selected: oral and dermal. No risk was found to young children’s health.
Changes in Metabolism and Content of Chlorophyll in Common Duckweed (Lemna minor L.) Caused by Environmental Contamination with Fluorides
Authors: Kamiński Jan , Stachelska-Wierzchowska Alicja , Michalczyk Dariusz J. , Klimkowicz-Pawlas Agnieszka , Olkowska Ewa , Wolska Lidia , Piotrowicz-Cieślak Agnieszka I.
Published: May 2024

The impact of fluorine on plants remains poorly understood. We examined duckweed growth in extracts of soil contaminated with fluorine leached from chicken manure. Additionally, fluorine levels were analyzed in fresh manure, outdoor-stored manure, and soil samples at varying distances from the manure pile. Fresh manure contained 37–48 mg F− × kg−1, while soil extracts contained 2.1 to 4.9 mg F− × kg−1. We evaluated the physiological effects of fluorine on duckweed cultured on soil extracts or in 50% Murashige–Skoog (MS) medium supplemented with fluorine concentrations matching those in soil samples (2.1 to 4.9 mg F− × L−1), as well as at 0, 4, and 210 mg × L−1. Duckweed exposed to fluorine displayed similar toxicity symptoms whether in soil extracts or supplemented medium. Fluoride at concentrations of 2.1 to 4.9 mg F− × L−1 reduced the intact chlorophyll content, binding the porphyrin ring at position 32 without affecting Mg2+. This reaction resulted in chlorophyll a absorption peak shifted towards shorter wavelengths and formation of a new band of the F−-chlorophyll a complex at λ = 421 nm. Moreover, plants exposed to low concentrations of fluorine exhibited increased activities of aminolevulinic acid dehydratase and chlorophyllase, whereas the activities of both enzymes sharply declined when the fluoride concentration exceeded 4.9 mg × L−1. Consequently, fluorine damages chlorophyll a, disrupts the activity of chlorophyll-metabolizing enzymes, and diminishes the plant growth rate, even when the effects of these disruptions are too subtle to be discerned by the naked human eye.
Prediction of Individual Gas Yields of Supercritical Water Gasification of Lignocellulosic Biomass by Machine Learning Models
Authors: Khandelwal Kapil , Dalai Ajay K.
Published: May 2024

Supercritical water gasification (SCWG) of lignocellulosic biomass is a promising pathway for the production of hydrogen. However, SCWG is a complex thermochemical process, the modeling of which is challenging via conventional methodologies. Therefore, eight machine learning models (linear regression (LR), Gaussian process regression (GPR), artificial neural network (ANN), support vector machine (SVM), decision tree (DT), random forest (RF), extreme gradient boosting (XGB), and categorical boosting regressor (CatBoost)) with particle swarm optimization (PSO) and a genetic algorithm (GA) optimizer were developed and evaluated for prediction of H2, CO, CO2, and CH4 gas yields from SCWG of lignocellulosic biomass. A total of 12 input features of SCWG process conditions (temperature, time, concentration, pressure) and biomass properties (C, H, N, S, VM, moisture, ash, real feed) were utilized for the prediction of gas yields using 166 data points. Among machine learning models, boosting ensemble tree models such as XGB and CatBoost demonstrated the highest power for the prediction of gas yields. PSO-optimized XGB was the best performing model for H2 yield with a test R2 of 0.84 and PSO-optimized CatBoost was best for prediction of yields of CH4, CO, and CO2, with test R2 values of 0.83, 0.94, and 0.92, respectively. The effectiveness of the PSO optimizer in improving the prediction ability of the unoptimized machine learning model was higher compared to the GA optimizer for all gas yields. Feature analysis using Shapley additive explanation (SHAP) based on best performing models showed that (21.93%) temperature, (24.85%) C, (16.93%) ash, and (29.73%) C were the most dominant features for the prediction of H2, CH4, CO, and CO2 gas yields, respectively. Even though temperature was the most dominant feature, the cumulative feature importance of biomass characteristics variables (C, H, N, S, VM, moisture, ash, real feed) as a group was higher than that of the SCWG process condition variables (temperature, time, concentration, pressure) for the prediction of all gas yields. SHAP two-way analysis confirmed the strong interactive behavior of input features on the prediction of gas yields.
The Enhanced Performance of NiCuOOH/NiCu(OH)2 Electrode Using Pre-Conversion Treatment for the Electrochemical Oxidation of Ammonia
Authors: Yin Xuejiao , Wen Jiaxin , Zhao Jujiao , An Ran , Zhang Ruolan , Xiong Yin , Tao Yanzong , Wang Lingxin , Liu Yuhang , Zhou Huanyu , Huang Yuanyuan
Published: May 2024

Electrochemical oxidation of ammonia is an attractive process for wastewater treatment, hydrogen production, and ammonia fuel cells. However, the sluggish kinetics of the anode reaction has limited its applications, leading to a high demand for novel electrocatalysts. Herein, the electrode with the in situ growth of NiCu(OH)2 was partially transformed into the NiCuOOH phase by a pre-treatment using highly oxidative solutions. As revealed by SEM, XPS, and electrochemical analysis, such a strategy maintained the 3D structure, while inducing more active sites before the in situ generation of oxyhydroxide sites during the electrochemical reaction. The optimized NiCuOOH-1 sample exhibited the current density of 6.06 mA cm−2 at 0.5 V, which is 1.67 times higher than that of NiCu(OH)2 (3.63 mA cm−2). Moreover, the sample with a higher crystalline degree of the NiCuOOH phase exhibited lower performance, demonstrating the importance of a moderate treatment condition. In addition, the NiCuOOH-1 sample presented low selectivity (<20%) towards NO2− and stable activity during the long-term operation. The findings of this study would provide valuable insights into the development of transition metal electrocatalysts for ammonia oxidation.
Effects of Graphene Oxide on Endophytic Bacteria Population Characteristics in Plants from Soils Contaminated by Polycyclic Aromatic Hydrocarbons
Authors: Zhou Xingxing , Zhang Bo , Meng Qingzhu , Li Lingmei
Published: May 2024

Environmental pollution stands as one of the significant global challenges we face today. Polycyclic aromatic hydrocarbons (PAHs), a class of stubborn organic pollutants, have long been a focal point of bioremediation research. This study aims to explore the impact and mechanisms of graphene oxide (GO) on the phytoremediation effectiveness of PAHs. The results underscore the significant efficacy of GO in accelerating the degradation of PAHs. Additionally, the introduction of GO altered the diversity and community structure of endophytic bacteria within the roots, particularly those genera with potential for PAH degradation. Through LEfSe analysis and correlation studies, we identified specific symbiotic bacteria, such as Mycobacterium, Microbacterium, Flavobacterium, Sphingomonas, Devosia, Bacillus, and Streptomyces, which coexist and interact under the influence of GO, synergistically degrading PAHs. These bacteria may serve as key biological markers in the PAH degradation process. These findings provide new theoretical and practical foundations for the application of nanomaterials in plant-based remediation of polluted soils and showcase the immense potential of plant–microbe interactions in environmental restoration.
Efficacy of Glycyrrhetinic Acid in the Treatment of Acne Vulgaris Based on Network Pharmacology and Experimental Validation
Authors: Xie Lingna , Ma Congwei , Li Xinyu , Chen Huixiong , Han Ping , Lin Li , Huang Weiqiang , Xu Menglu , Lu Hailiang , Du Zhiyun
Published: May 2024

Glycyrrhetinic acid (GA) is a saponin compound, isolated from licorice (Glycyrrhiza glabra), which has been wildly explored for its intriguing pharmacological and medicinal effects. GA is a triterpenoid glycoside displaying an array of pharmacological and biological activities, including anti-inflammatory, anti-bacterial, antiviral and antioxidative properties. In this study, we investigated the underlying mechanisms of GA on acne vulgaris through network pharmacology and proteomics. After the intersection of the 154 drug targets and 581 disease targets, 37 therapeutic targets for GA against acne were obtained. A protein–protein interaction (PPI) network analysis highlighted TNF, IL1B, IL6, ESR1, PPARG, NFKB1, STAT3 and TLR4 as key targets of GA against acne, which is further verified by molecular docking. The experimental results showed that GA inhibited lipid synthesis in vitro and in vivo, improved the histopathological damage of skin, prevented mast cell infiltration and decreased the level of pro-inflammatory cytokines, including TNF-α, IL-1β and IL-6. This study indicates that GA may regulate multiple pathways to improve acne symptoms, and the beneficial effects of GA against acne vulgaris might be through the regulation of sebogenesis and inflammatory responses.