bluuebunny/crossref_metadata_2025 huggingface dataset:
Astragaloside IV Protects Against Oxidative Stress in Calf Small Intestine Epithelial Cells via NFE2L2-Antioxidant Response Element Signaling
Authors: Wang Yafang , Jiang Fugui , Cheng Haijian , Tan Xiuwen , Liu Yifan , Wei Chen , Song Enliang
Published: December 2019

Oxidative stress can damage intestinal epithelial cell integrity and function, causing gastrointestinal disorders. Astragaloside IV (ASIV) exhibits a variety of biological and pharmacological properties, including anti-inflammatory and antioxidant effects. The purpose of this research was to investigate the cytoprotective action of ASIV and its mechanisms in calf small intestine epithelial cells with hydrogen peroxide (H2O2)-induced oxidative stress. ASIV pretreatment not only increased cell survival, but it also decreased reactive oxygen species generation and apoptosis, enhanced superoxide dismutase, catalase, and glutathione peroxidase levels, and it reduced malondialdehyde formation. Furthermore, pretreatment with ASIV elevated the mRNA and protein levels of nuclear factor erythroid 2-related factor 2 (NFE2L2), heme oxygenase-1 (HMOX1), and NAD(P)H quinone dehydrogenase 1 (NQO1). The NFE2L2 inhibitor ML385 inhibited NFE2L2 expression and then blocked HMOX1 and NQO1 expression. These results demonstrate that ASIV treatment effectively protects against H2O2-induced oxidative damage in calf small intestine epithelial cells through the activation of the NFE2L2-antioxidant response element signaling pathway.
Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease
Authors: Peña-Bautista Carmen , Tirle Tania , López-Nogueroles Marina , Vento Máximo , Baquero Miguel , Cháfer-Pericás Consuelo
Published: December 2019

Alzheimer’s Disease (AD) is the most common cause of dementia, and its characteristic histopathological hallmarks are neurofibrillary tangles and senile plaques. Among involved mechanisms, oxidative stress plays an important role in damaging cell components (e.g., proteins, nucleic acids). In this study, different oxidized products of proteins and DNA were determined in the urine samples from mild cognitive impairment due to AD patients (n = 53) and healthy controls (n = 27) by means of ultra-performance liquid chromatography-tandem mass spectrometry analysis. A multivariate model developed by partial least squares generated a diagnostic model for AD with an AUC-ROC (area under the curve-receiver operating characteristic) of 0.843. From the studied analytes, 8-OHdG (8-hydroxy-2’-deoxyguanosine) and the ratio 8-OHdG/2dG (2’-deoxyguanosine) were able to distinguish between AD and healthy participants, showing statistically significant differences between groups, postulating DNA oxidation as a molecular pathway involved in early AD.
Mechanisms of Disease Progression and Resistance to Tyrosine Kinase Inhibitor Therapy in Chronic Myeloid Leukemia: An Update
Authors: Bavaro Luana , Martelli Margherita , Cavo Michele , Soverini Simona
Published: December 2019

Chronic myeloid leukemia (CML) is characterized by the presence of the BCR-ABL1 fusion gene, which encodes a constitutive active tyrosine kinase considered to be the pathogenic driver capable of initiating and maintaining the disease. Despite the remarkable efficacy of tyrosine kinase inhibitors (TKIs) targeting BCR-ABL1, some patients may not respond (primary resistance) or may relapse after an initial response (secondary resistance). In a small proportion of cases, development of resistance is accompanied or shortly followed by progression from chronic to blastic phase (BP), characterized by a dismal prognosis. Evolution from CP into BP is a multifactorial and probably multistep phenomenon. Increase in BCR-ABL1 transcript levels is thought to promote the onset of secondary chromosomal or genetic defects, induce differentiation arrest, perturb RNA transcription, editing and translation that together with epigenetic and metabolic changes may ultimately lead to the expansion of highly proliferating, differentiation-arrested malignant cells. A multitude of studies over the past two decades have investigated the mechanisms underlying the closely intertwined phenomena of drug resistance and disease progression. Here, we provide an update on what is currently known on the mechanisms underlying progression and present the latest acquisitions on BCR-ABL1-independent resistance and leukemia stem cell persistence.
Discovery of a Highly Selective MC1R Agonists Pentapeptide to Be Used as a Skin Pigmentation Enhancer and with Potential Anti-Aging Properties
Authors: Jackson Eileen , Heidl Marc , Imfeld Dominik , Meeus Laurent , Schuetz Rolf , Campiche Remo
Published: December 2019

One of the first lines of cutaneous defense against photoaging is (a) the synthesis of melanin and (b) the initiation of an oxidative stress response to protect skin against the harmful effects of solar radiation. Safe and selective means to stimulate epidermal pigmentation associated with oxidative stress defense are; however, scarce. Activation of the melanocortin-1 receptor (MC1R) on epidermal melanocytes represents a key step in cutaneous pigmentation initiation and, additionally, it regulates cellular defense mechanisms like oxidative stress and DNA-repair. Thus, making the activation of MC1R an attractive strategy for modulating skin pigmentation and oxidative stress. In this context, we designed and synthesized pentapeptides that act as MC1R agonists. These peptides bound, with high potency, to MC1R and activated cAMP synthesis in CHO cells expressing human MC1R. Using one lead pentapeptide, we could show that this activation of MC1R was specific as testing the activation of other G-protein coupled receptors, including the MC-receptor family, was negative. In vitro efficacy on mouse melanoma cells showed similar potency as for the synthetic MC1R agonist alpha-melanocyte stimulating hormone (NDP-alpha-MSH). Moreover, we could reproduce this activity in human skin tissue culture. The lead pentapeptide was able to induce ex-vivo protein expression of key melanogenesis markers melanocyte inducing transcription factor (MITF), tyrosinase (TYR), and tyrosinase-related protein 1 (TYRP-1). Concerning oxidative stress response, we found that the pentapeptide enhanced the activation of Nrf2 after UVA-irradiation. Our results make this pentapeptide an ideal candidate as a skin pigmentation enhancer that mimics alpha-MSH and may also have anti-photoaging effects on the skin.
Windup of Nociceptive Flexion Reflex Depends on Synaptic and Intrinsic Properties of Dorsal Horn Neurons in Adult Rats
Authors: Aby Franck , Bouali-Benazzouz Rabia , Landry Marc , Fossat Pascal
Published: December 2019

Windup, a progressive increase in spinal response to repetitive stimulations of nociceptive peripheral fibers, is a useful model to study central sensitization to pain. Windup is expressed by neurons in both the dorsal and ventral horn of the spinal cord. In juvenile rats, it has been demonstrated both in vivo and in vitro that windup depends on calcium-dependent intrinsic properties and their modulation by synaptic components. However, the involvement of these two components in the adults remains controversial. In the present study, by means of electromyographic and extracellular recordings, we show that windup in adults, in vivo, depends on a synaptic balance between excitatory N-methyl-D-aspartate (NMDA) receptors and inhibitory glycinergic receptors. We also demonstrate the involvement of L-type calcium channels in both the dorsal and ventral horn of the spinal cord. These results indicate that windup in adults is similar to juvenile rats and that windup properties are the same regardless of the spinal network, i.e., sensory or motor.
Leaf Morphological and Biochemical Responses of Three Potato (Solanum tuberosum L.) Cultivars to Drought Stress and Aphid (Myzus persicae Sulzer) Infestation
Authors: Quandahor Peter , Lin Chunyan , Gou Yuping , A. Coulter Jeffrey , Liu Changzhong
Published: December 2019

Drought stress on plants can cause cellular water deficits and influence the physiology of host plants, which alter the performance of insect pests. This study was conducted to determine the effect of drought and aphid (Myzus persicae Sulzer) infestation on three potato (Solanum tuberosum L.) genotypes under greenhouse conditions. A factorial experiment involving three potato genotypes, two levels of drought, and two levels of aphid infestation was conducted. The potato genotypes possessed different levels of tolerance to drought and are described as tolerant (Qingshu 9), moderately tolerant (Longshu 3), and sensitive (Atlantic). Sixty-day-old potato plants were infested with aphid nymphs and monitored for 20 d. There was a significant variety × drought × aphid interaction effect on the parameters measured. The genotype Atlantic, which is sensitive to drought, exhibited greater tolerance to aphid infestation under drought or no drought conditions than the other genotypes. This genotype also exhibited poor host acceptance and the aphid survival rate, colonization success, and average daily reproduction were low. Qingshu 9, which is tolerant to drought, was highly susceptible to aphid infestation and exhibited high host acceptance and greater aphid survival rate, colonization success, and average daily reproduction compared to the other genotypes. This study demonstrates that the biochemical and morphological traits that confer drought tolerance in potato do not necessarily confer aphid tolerance.
Stag Beetle Elytra: Localized Shape Retention and Puncture/Wear Resistance
Authors: Kundanati Lakshminath , Guarino Roberto , Pugno Nicola M.
Published: December 2019

Beetles are by far one of the most successful groups of insects, with large diversity in terms of number of species. A part of this success is attributed to their elytra, which provide various functions such as protection to their bodies from mechanical forces. In this study, stag beetle (Lucanus cervus) elytra were first examined for their overall flexural properties and were observed to have a localized shape-retaining snap-through mechanism, which may play a possible role in partly absorbing impact energy, e.g., during battles and falls from heights. The snap-through mechanism was validated using theoretical calculations and also finite element simulations. Elytra were also characterized to examine their puncture and wear resistance. Our results show that elytra have a puncture resistance that is much higher than that of mandible bites. The measured values of modulus and hardness of elytra exocuticle were 10.3 ± 0.8 GPa and 0.7 ± 0.1 GPa, respectively. Using the hardness-to-modulus ratio as an indicator of wear resistance, the estimated value was observed to be in the range of wear-resistant biological material such as blood worms (Glyrcera dibranchiata). Thus, our study demonstrates different mechanical properties of the stag beetle elytra, which can be explored to design shape-retaining bio-inspired composites with enhanced puncture and wear resistance.
Study of the Extracted Beam Energy as a Function of Operational Parameters of a Medical Cyclotron
Authors: Häffner Philipp , Belver Aguilar Carolina , Braccini Saverio , Scampoli Paola , Thonet Pierre Alexandre
Published: December 2019

The medical cyclotron at the Bern University Hospital (Inselspital) is used for both routine 18 F production for Positron Emission Tomography (PET) and multidisciplinary research. It provides proton beams of variable intensity at a nominal fixed energy of 18 MeV. Several scientific activities, such as the measurement of nuclear reaction cross-sections or the production of non-conventional radioisotopes for medical applications, require a precise knowledge of the energy of the beam extracted from the accelerator. For this purpose, a study of the beam energy was performed as a function of cyclotron operational parameters, such as the magnetic field in the dipole magnet or the position of the extraction foil. The beam energy was measured at the end of the 6 m long Beam Transfer Line (BTL) by deflecting the accelerated protons by means of a dipole electromagnet and by assessing the deflection angle with a beam profile detector.
Short and Long-Term Effectiveness of Ustekinumab in Patients with Crohn’s Disease: Real-World Data from a German IBD Cohort
Authors: Kubesch Alica , Rueter Laurenz , Farrag Karima , Krause Thomas , Stienecker Klaus , Hausmann Johannes , Filmann Natalie , Dignass Axel , Stein Jürgen , Blumenstein Irina
Published: December 2019

Background and Aims: The IL-12/23 inhibitor ustekinumab (UST) opened up new treatment options for patients with Crohn’s disease (CD). Due to the recent approval, real-world German data on long-term efficacy and safety are lacking. This study aimed to assess the clinical course of CD patients under UST therapy and to identify potential predictive markers. Methods: Patients with CD receiving UST treatment in three hospitals and two outpatient centers were included and retrospectively analyzed. Rates for short- and long-term remission and response were analyzed with the help of clinical (Harvey–Bradshaw Index (HBI)) and biochemical (C-reactive protein (CRP), Fecal calprotectin (fCal)) parameters for disease activity. Results: Data from 180 patients were evaluated. One-hundred-and-six patients had a follow-up of at least eight weeks and were included. 96.2% of the patients were pre-exposed to anti- TNFα agents and 34.4% to both anti-TNFα and anti-integrin antibodies. The median follow-up was 49.1 weeks (95% CI 42.03-56.25). At week 8, 51 patients (54.8%) showed response to UST, and 24 (24.7%) were in remission. At week 48, 48 (51.6%) responded to UST, and 25 patients (26.9%) were in remission. Steroid-free response and remission at week eight was achieved by 30.1% and 19.3% of patients, respectively. At week 48, 37.6% showed steroid-free response to UST, and 20.4% of the initial patient population was in steroid-free remission. Conclusion: Our study confirms short- and long-term UST effectiveness and tolerability in a cohort of multi-treatment-exposed patients.
Disconnected Body Representation: Neuroplasticity Following Spinal Cord Injury
Authors: Leemhuis Erik , De Gennaro Luigi , Pazzaglia and Mariella
Published: December 2019

Neuroplastic changes in somatotopic organization within the motor and somatosensory systems have long been observed. The interruption of afferent and efferent brain–body pathways promotes extensive cortical reorganization. Changes are majorly related to the typical homuncular organization of sensorimotor areas and specific “somatotopic interferences”. Recent findings revealed a relevant peripheral contribution to the plasticity of body representation in addition to the role of sensorimotor cortices. Here, we review the ways in which structures and brain mechanisms react to missing or critically altered sensory and motor peripheral signals. We suggest that these plastic events are: (i) variably affected across multiple timescales, (ii) age-dependent, (iii) strongly related to altered perceptual sensations during and after remapping of the deafferented peripheral area, and (iv) may contribute to the appearance of secondary pathological conditions, such as allodynia, hyperalgesia, and neuropathic pain. Understanding the considerable complexity of plastic reorganization processes will be a fundamental step in the formulation of theoretical and clinical models useful for maximizing rehabilitation programs and resulting recovery.
Fat Mass Index Better Identifies Metabolic Syndrome: Insights from Patients in Early Outpatient Cardiac Rehabilitation
Authors: Bonikowske Amanda R. , Barillas Lara Maria Irene , Koepp Katlyn E. , Medina Inojosa Jose R. , Squires Ray W. , Lopez-Jimenez Francisco , Olson Thomas P.
Published: December 2019

Body mass index (BMI) does not differentiate fat and lean mass or the distribution of adipose tissue. The purpose of this study was to examine the prevalence of metabolic syndrome (MetS) among patients entering outpatient cardiac rehabilitation (CR) across fat mass index (FMI) categories compared with BMI. This retrospective cross-sectional study evaluated dual-energy x-ray absorptiometry in 483 CR patients from 1 January 2014, through 31 December 2017. Clinical data were extracted from the electronic health record. Patients were grouped by FMI and BMI categories. Mean (SD) age of patients was 64.3 (14) years. The normal FMI category had 15 patients; excess fat, 74; and obese, 384. In contrast, 93, 174, and 216 were in the normal, overweight, and obese BMI categories, respectively. Prevalence of MetS was 0 (0%) in normal, 5 (1%) in excess fat, and 167 (54%) in obese FMI, with 97% in the obese category. MetS prevalence was 4 patients (0.8%) in normal, 39 (8%) in overweight, and 129 (27%) in obese BMI categories, with 75% of MetS in the obese category. FMI more accurately classified CR patients with metabolically abnormal fat (p < 0.001). FMI is a more sensitive index than BMI for metabolically abnormal fat of outpatient CR patients.
Predicting Long-Term Health-Related Quality of Life after Bariatric Surgery Using a Conventional Neural Network: A Study Based on the Scandinavian Obesity Surgery Registry
Authors: Cao Yang , Raoof Mustafa , Montgomery Scott , Ottosson Johan , Näslund Ingmar
Published: December 2019

Severe obesity has been associated with numerous comorbidities and reduced health-related quality of life (HRQoL). Although many studies have reported changes in HRQoL after bariatric surgery, few were long-term prospective studies. We examined the performance of the convolution neural network (CNN) for predicting 5-year HRQoL after bariatric surgery based on the available preoperative information from the Scandinavian Obesity Surgery Registry (SOReg). CNN was used to predict the 5-year HRQoL after bariatric surgery in a training dataset and evaluated in a test dataset. In general, performance of the CNN model (measured as mean squared error, MSE) increased with more convolution layer filters, computation units, and epochs, and decreased with a larger batch size. The CNN model showed an overwhelming advantage in predicting all the HRQoL measures. The MSEs of the CNN model for training data were 8% to 80% smaller than those of the linear regression model. When the models were evaluated using the test data, the CNN model performed better than the linear regression model. However, the issue of overfitting was apparent in the CNN model. We concluded that the performance of the CNN is better than the traditional multivariate linear regression model in predicting long-term HRQoL after bariatric surgery; however, the overfitting issue needs to be mitigated using more features or more patients to train the model.
Robust Bayesian Inference in Stochastic Frontier Models
Authors: Tsionas Mike G.
Published: December 2019

We use the concept of coarsened posteriors to provide robust Bayesian inference via coarsening in order to robustify posteriors arising from stochastic frontier models. These posteriors arise from tempered versions of the likelihood when at most a pre-specified amount of data is used, and are robust to changes in the model. Specifically, we examine robustness to changes in the distribution of the composed error in the stochastic frontier model (SFM). Moreover, coarsening is a form of regularization, reduces overfitting and makes inferences less sensitive to model choice. The new techniques are illustrated using artificial data as well as in a substantive application to large U.S. banks.
Power of Agricultural Credit in Farmland Abandonment: Evidence from Rural China
Authors: Du Jiang , Zeng Miao , Xie Zhengjuan , Wang Shikun
Published: December 2019

Labor, land, and funds are keys to revitalizing rural areas around the world. Previous studies have focused on the impacts of funds on agricultural production, but placed little emphasis on its role in agricultural land-use transformation. Thus, this study explores the quantitative relationship between agricultural credit and farmland abandonment from the perspective of rural revitalization. Using data on 8031 households from 27 provinces obtained from China’s Labor Force Dynamics Survey (CLDS), this study uses a Tobit model to examine the quantitative impacts of informal and formal agricultural credit on farmland abandonment. The results indicate that: (1) Access to agricultural credit helps to reduce farmland abandonment. (2) Compared with formal agricultural credit (provided by institutions), informal agricultural credit (provided by family and friends) is more significant in reducing farmland abandonment. Thus, this study enhances our understanding of the relationship between agricultural credit and farmland use. It will also prompt policymakers to improve rural financial markets in order to reduce the misallocation of farmland resources, thereby improving food security and rural economies.
Household Land Allocations and the Youth Land Access Nexus: Evidence from the Techiman Area of Ghana
Authors: Kidido Joseph Kwaku , Lengoiboni Monica
Published: December 2019

Building inclusive societies that reflect the needs of all categories of people within the social spectrum is critical to achieving sustainable development. This is reflected in the Sustainable Development Goals (SDGs) which among things seek to ‘by 2030, empower and promote the social, economic and political inclusion of all, irrespective of age, sex. This places enormous tasks on all governments especially in developing countries like Ghana to ensure that the youth are not left behind in access and control over land as a building block for economic empowerment. This task is particularly critical in view of the sheer numbers of the youth and yet economically marginalized underpinned by high levels of unemployment and underemployment. This case study investigates the youth land rights within the context of household landholdings and allocations dynamics. The study took place in the Techiman area in Ghana. The study sampled 455 youth and 138 household heads. The study revealed that household lands are important building block for majority of the youth in the Techiman area. It gives them a sense of security in the usage. However, the youth’s ability to depend on this source to kick start independence economic life is beset with land scarcity, non-allocation and accumulation by the lineage heads who have prerogative over household lands. The study underscores the need for social welfare scheme for the aged farmers so that they can timely release land to the younger ones without fearing for what to sustain them. There is also the need for government to create land banks to support the willing youth to engage in agriculture.
An Experimental Study of the Frictional Properties of Steel Sheets Using the Drawbead Simulator Test
Authors: Trzepiecinski Tomasz , Kubit Andrzej , Slota Ján , Fejkiel Romuald
Published: December 2019

This article presents the results of an experimental investigation of the frictional resistance arising in a drawbead during sheet metal forming. The frictional characterization of DC04 deep drawing quality steels commonly used in the automotive industry is carried out using a friction simulator. The effects of some parameters of the friction process on the value of the coefficient of friction have been considered in the experimental investigations. The friction tests have been conducted on different strip specimens, lubrication conditions, heights of drawbead and specimen orientations in relation to the sheet rolling direction. The results of drawbead simulator tests demonstrate the relationship that the value of the coefficient of friction of the test sheets without lubrication is higher than in the case of lubricated sheets. The lubricant reduces the coefficient of friction, but the effectiveness of its reduction depends on the drawbead height and lubrication conditions. Moreover, the effectiveness of the reduction of the coefficient of friction by the lubricant depends on the specimen orientation according to the sheet rolling direction. In the drawbead test, the specimens oriented along the rolling direction demonstrate a higher value of coefficient of friction when compared to the samples cut transverse to the rolling direction. The smaller the width of the specimen, the lower the coefficient of friction observed. The difference in the coefficient of friction for the extreme values of the widths of the specimens was about 0.03–0.05. The use of machine oil reduced the coefficient of friction by 0.02–0.03 over the whole range of drawbead heights. Heavy duty lubricant even reduced the frictional resistances by over 50% compared to dry friction conditions. The effectiveness of friction reduction by machine oil does not exceed 30%.
The Effect of the Feed Direction on the Micro- and Macro Accuracy of 3D Ball-end Milling of Chromium-Molybdenum Alloy Steel
Authors: Mikó Balázs , Varga Bálint , Zębala Wojciech
Published: December 2019

The machining of free form surfaces is one of the most challenging problems in the field of metal cutting technology. The produced part and machining process should satisfy the working, accuracy, and financial requirements. The accuracy can describe dimensional, geometrical, and surface roughness parameters. In the current article, three of them are investigated in the case of the ball-end milling of a convex and concave cylindrical surface form 42CrMo4 steel alloy. The effect of the tool path direction is investigated and the other cutting parameters are constant. The surface roughness and the geometric error are measured by contact methods. Based on the results, the surface roughness, dimensional error, and the geometrical error mean different aspects of the accuracy, but they are not independent from each other. The investigated input parameters have a similar effect on them. The regression analyses result a very good liner regression for geometric errors and shows the importance of surface roughness.
Complete Killing of Agar Lawn Biofilms by Systematic Spacing of Antibiotic-Loaded Calcium Sulfate Beads
Authors: Dusane Devendra H. , Brooks Jacob R. , Sindeldecker Devin , Peters Casey W. , Li Anthony , Farrar Nicholas R. , Diamond Scott M. , Knecht Cory S. , Plaut Roger D. , Delury Craig , Aiken Sean S. , Laycock Phillip A. , Sullivan Anne , Granger Jeffrey F. , Stoodley Paul
Published: December 2019

Background: Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) are the major causative agents of acute and chronic infections. Antibiotic-loaded calcium sulfate beads (ALCSB) are used in the management of musculoskeletal infections such as periprosthetic joint infections (PJI). Methods: To determine whether the number and spatial distribution of ALCSB are important factors to totally eradicate biofilms, ALCSBs containing vancomycin and tobramycin were placed on 24 h agar lawn biofilms as a single bead in the center, or as 16 beads placed as four clusters of four, a ring around the edge and as a group in the center or 19 beads evenly across the plate. Bioluminescence was used to assess spatial metabolic activity in real time. Replica plating was used to assess viability. Results: For both strains antibiotics released from the beads completely killed biofilm bacteria in a zone immediately adjacent to each bead. However, for PA extended incubation revealed the emergence of resistant colony phenotypes between the zone of eradication and the background lawn. The rate of biofilm clearing was greater when the beads were distributed evenly over the plate. Conclusions: Both number and distribution pattern of ALCSB are important to ensure adequate coverage of antibiotics required to eradicate biofilms.
Release of Antibiotics Out of a Moldable Collagen-β-Tricalciumphosphate-Composite Compared to Two Calcium Phosphate Granules
Authors: Roth Klaus Edgar , Maier Gerrit Steffen , Schmidtmann Irene , Eigner Ulrich , Hübner Wolf Dietrich , Peters Fabian , Drees Philipp , Maus Uwe
Published: December 2019

Bacterial bone infections after revision surgeries and diseases, like osteomyelitis, are still a challenge with regard to surgical treatments. Local bone infections were treated with antibiotics directly or by controlled drug-releasing scaffolds, like polymethylmethacrylate (PMMA) spheres, which have to be removed at a later stage, but there is a risk of a bacterial contamination during the removement. Therefore, biomaterials loaded with antibiotics for controlled release could be the method of choice: The biomaterials degrade during the drug release, therefore, there is no need for a second surgery to remove the drug eluting agent. Even non-resorbable bone materials are available (e.g., hydroxyapatite (HA)) or resorbable bone graft materials (e.g., beta-tricalcium phosphate (β-TCP)) that will be replaced by newly formed bone. Composite materials with organic additives (e.g., collagen) supports the handling during surgery and enhances the drug loading capacity, as well as the drug releasing time. The purpose of this study was to investigate the loading capacity and the release rate of Vancomycin and Gentamicin on TCP and HA granules in the shape of a degradable scaffold compared to composite materials from TCP mixed with porcine collagen. Its antibacterial efficacy to a more elementary drug with eluting in aqueous solution was examined. The loading capacity of the biomaterials was measured and compared according to the Minimum Inhibition Concentration (MIC) and the Minimum Biofilm Eradication Concentration (MBEC) of a bacterial biofilm after 24 h aging. Antibiotic elution and concentration of gentamycin and vancomycin, as well as inhibition zones, were measured by using the Quantitative Microparticle Systems (QMS) immunoassays. The antibiotic concentration was determined by the automated Beckman Coulter (BC) chemistry device. For examination of the antibacterial activity, inhibition zone diameters were measured. Generally, the antibiotic release is more pronounced during the first couple of days than later. Both TCP granules and HA granules experienced a significantly decline of antibiotics release during the first three days. After the fourth day and beyond, the antibiotic release was below the detection threshold. The antibiotic release of the composite material TCP and porcine collagen declined less drastically and was still in the frame of the specification during the first nine days. There was no significant evidence of interaction effect between antibiotic and material, i.e., the fitted lines for Gentamycin and Vancomycin are almost parallel. During this first in vitro study, β-TCP-Collagen composites shows a significantly higher loading capacity and a steadily release of the antibiotics Gentamycin and Vancomycin, compared to the also used TCP and HA Granules.
A Photoelectrochemical Study of Bioinspired 2-Styryl-1-Benzopyrylium Cations on TiO2 Nanoparticle Layer for Application in Dye-Sensitized Solar Cells
Authors: Calogero Giuseppe , Citro Ilaria , Calandra Sebastianella Gioacchino , Di Marco Gaetano , Diniz Ana Marta , Parola A. Jorge , Pina Fernando
Published: December 2019

In the present work, five 2-styryl-1-benzopyrylium salts and their relative self-assembly processes towards TiO2 nanocrystalline layers were evaluated as photosensitizers in dye-sensitized solar cells (DSSCs). Integration of these 2-styryl-1-benzopyrylium salts with the semiconductor allow for the performance of highly specific functions suitable for smart applications in material science. Spectroscopic and photoelectrochemical measurements conducted on these five bio-inspired dyes, in solution and upon adsorption onto titanium dioxide films, allowed detailed discussion of the anchoring ability of the different donor groups decorating the 2-styryl-1-benzopyrylium core and have demonstrated their ability as photosensitizers. Our results suggest that the introduction of a dimethylamino group in position 4′ of the 2-styryl-1-benzopyrylium skeleton can alter the conjugation of the molecule leading to larger absorption in the visible region and a stronger electron injection of the dye into the conduction band of TiO2. Moreover, our experimental data have been supported by theoretical calculations with the aim to study the energy of the excited states of the five compounds. In this specific case, the simulations reported contributed to better describe the properties of the compounds used and to help create the necessary basis for the design of new and targeted bio-inspired molecules.
Comparative Machine-Learning Approach: A Follow-Up Study on Type 2 Diabetes Predictions by Cross-Validation Methods
Authors: Battineni Gopi , Sagaro Getu Gamo , Nalini Chintalapudi , Amenta Francesco , Tayebati Seyed Khosrow
Published: December 2019

(1) Background: Diabetes is a common chronic disease and a leading cause of death. Early diagnosis gives patients with diabetes the opportunity to improve their dietary habits and lifestyle and manage the disease successfully. Several studies have explored the use of machine learning (ML) techniques to predict and diagnose this disease. In this study, we conducted experiments to predict diabetes in Pima Indian females with particular ML classifiers. (2) Method: A Pima Indian diabetes dataset (PIDD) with 768 female patients was considered for this study. Different data mining operations were performed to a conduct comparative analysis of four different ML classifiers: Naïve Bayes (NB), J48, Logistic Regression (LR), and Random Forest (RF). These models were analyzed by different cross-validation (K = 5, 10, 15, and 20) values, and the performance measurements of accuracy, precision, F-score, recall, and AUC were calculated for each model. (3) Results: LR was found to have the highest accuracy (0.77) for all ‘k’ values. When k = 5, the accuracy of J48, NB, and RF was found to be 0.71, 0.76, and 0.75. For k = 10, the accuracy of J48, NB, and RF was found to be 0.73, 0.76, 0.74, while for k = 15, 20, the accuracy of NB was found to be 0.76. The accuracy of J48 and RF was found to be 0.76 when k = 15, and 0.75 when k = 20. Other parameters, such as precision, f-score, recall, and AUC, were also considered in evaluations to rank the algorithms. (4) Conclusion: The present study on PIDD sought to identify an optimized ML model, using with cross-validation methods. The AUC of LR was 0.83, RF 0.82, and NB 0.81). These three were ranked as the best models for predicting whether a patient is diabetic or not.
Review of Some Control Theory Results on Uniform Stability of Impulsive Systems
Authors: Liu Bin , Xu Bo , Zhang Guohua , Tong Lisheng
Published: December 2019

This paper aims to review some uniform stability results for impulsive systems. For the review, we classify the models of impulsive systems into time-based impulsive systems and state-based ones, including continuous-time impulsive systems, discrete-time impulsive systems, stochastic impulsive systems, and impulsive hybrid systems. According to these models, we review, respectively, the related stability concepts and some representative results focused on uniform stability, including the results on uniform asymptotic stability, input-to-state stability (ISS), KLL -stability (uniform stability expressed by KLL -functions), event-stability, and event-ISS. And we formulate some questions for those not fully developed aspects on uniform stability at each subsection.
Using Dynamic Adjusting NGHS-ANN for Predicting the Recidivism Rate of Commuted Prisoners
Authors: Shih Po-Chou , Chiu Chui-Yu , Chou Chi-Hsun
Published: December 2019

Commutation is a judicial policy that is implemented in most countries. The recidivism rate of commuted prisoners directly affects people’s perceptions and trust of commutation. Hence, if the recidivism rate of a commuted prisoner could be accurately predicted before the person returns to society, the number of reoffences could be reduced; thereby, enhancing trust in the process. Therefore, it is of considerable importance that the recidivism rates of commuted prisoners are accurately predicted. The dynamic adjusting novel global harmony search (DANGHS) algorithm, as proposed in 2018, is an improved algorithm that combines dynamic parameter adjustment strategies and the novel global harmony search (NGHS). The DANGHS algorithm improves the searching ability of the NGHS algorithm by using dynamic adjustment strategies for genetic mutation probability. In this paper, we combined the DANGHS algorithm and an artificial neural network (ANN) into a DANGHS-ANN forecasting system to predict the recidivism rate of commuted prisoners. To verify the prediction performance of the DANGHS-ANN algorithm, we compared the experimental results with five other forecasting systems. The results showed that the proposed DANGHS-ANN algorithm gave more accurate predictions. In addition, the use of the threshold linear posterior decreasing strategy with the DANGHS-ANN forecasting system resulted in more accurate predictions of recidivism. Finally, the metaheuristic algorithm performs better searches with the dynamic parameter adjustment strategy than without it.
Modeling and Optimal Control on the Spread of Hantavirus Infection
Authors: Mohamed Yusof Fauzi , Abdullah Farah Aini , Md. Ismail Ahmad Izani
Published: December 2019

In this paper, optimal control theory is applied to a system of ordinary differential equations representing a hantavirus infection in rodent and alien populations. The effect of the optimal control in eliminating the rodent population that caused the hantavirus infection is investigated. In addition, Pontryagin’s maximum principle is used to obtain the necessary condition for the controls to be optimal. The Runge–Kutta method is then used to solve the proposed optimal control system. The findings from the optimal control problem suggest that the infection may be eradicated by implementing some controls for a certain period of time. This research concludes that the optimal control mathematical model is an effective method in reducing the number of infectious in a community and environment.
A 2D Non-Linear Second-Order Differential Model for Electrostatic Circular Membrane MEMS Devices: A Result of Existence and Uniqueness
Authors: Di Barba Paolo , Fattorusso Luisa , Versaci Mario
Published: December 2019

In the framework of 2D circular membrane Micro-Electric-Mechanical-Systems (MEMS), a new non-linear second-order differential model with singularity in the steady-state case is presented in this paper. In particular, starting from the fact that the electric field magnitude is locally proportional to the curvature of the membrane, the problem is formalized in terms of the mean curvature. Then, a result of the existence of at least one solution is achieved. Finally, two different approaches prove that the uniqueness of the solutions is not ensured.
Submanifolds in Normal Complex Contact Manifolds
Authors: Mihai Adela , Mihai Ion
Published: December 2019

In the present article we initiate the study of submanifolds in normal complex contact metric manifolds. We define invariant and anti-invariant ( C C -totally real) submanifolds in such manifolds and start the study of their basic properties. Also, we establish the Chen first inequality and Chen inequality for the invariant δ ( 2 , 2 ) for C C -totally real submanifolds in a normal complex contact space form and characterize the equality cases. We also prove the minimality of C C -totally real submanifolds of maximum dimension satisfying the equalities.
Cytotoxic Polyketides Isolated from the Deep-Sea-Derived Fungus Penicillium chrysogenum MCCC 3A00292
Authors: Niu Siwen , Xia Manli , Chen Mingliang , Liu Xiupian , Li Zengpeng , Xie Yunchang , Shao Zongze , Zhang Gaiyun
Published: December 2019

The chemical examination of the solid cultures of the deep-sea-derived fungus Penicillium chrysogenum MCCC 3A00292 resulted in the isolation of three new versiol-type analogues, namely peniciversiols A–C (1–3), and two novel lactone derivatives, namely penicilactones A and B (6 and 7), along with 11 known polyketides. The planar structures of the new compounds were determined by the comprehensive analyses of the high-resolution electrospray ionization mass spectroscopy (HRESIMS) and nuclear magnetic resonance (NMR) data, while their absolute configurations were resolved on the basis of comparisons of the experimental electronic circular dichroism (ECD) spectra with the calculated ECD data. Compound 1 is the second example of versiols featuring a 2,3-dihydropyran-4-one ring. Additionally, compounds 6 and 7 are the first representatives of γ-lactone derivatives constructed by a 1,3-dihydroxy-5-methylbenzene unit esterifying with the α-methyl-γ-hydroxy-γ-acetic acid α,β-unsaturated-γ-lactone moiety and α-hydroxy-γ-methyl-γ-acetic acid α,β-unsaturated-γ-lactone unit, respectively. All of the isolated compounds were evaluated for their cytotoxic activities against five human cancer cell lines of BIU-87, ECA109, BEL-7402, PANC-1, and Hela-S3. Compound 1 exhibited a selective inhibitory effect against the BIU-87 cell line (IC50 = 10.21 μM), while compounds 4, 5, 8, and 12–16 showed inhibitory activities against the ECA109, BIU-87, and BEL-7402 cell lines with the IC50 values ranging from 7.70 to > 20 μM.
Prediction of Central Carbon Segregation in Continuous Casting Billet Using A Regularized Extreme Learning Machine Model
Authors: Zou Leilei , Zhang Jiangshan , Liu Qing , Zeng Fanzheng , Chen Jun , Guan Min
Published: December 2019

Central carbon segregation is a typical internal defect of continuous cast steel billets. Real-time and accurate carbon segregation prediction is of great significance for lean control of the production quality in continuous casting processes. In this paper, a data-driven regularized extreme learning machine (R-ELM) model is proposed for the prediction of carbon segregation index (CSI). To improve model performance, outliers in industrial data were eliminated by means of boxplot tool. Besides, Pearson correlation combined with grey relational analysis (GRA) was conducted to avoid multicollinearity and redundancy in input variables. The new model shows potential to evaluate online quality of steel billets. When predictive errors were within ±0.03 and ±0.025, the prediction accuracy of the R-ELM model was 94% and 89%, respectively, which was higher than that of the multiple linear regression (MLR) model and ELM model. Moreover, the effects of several key continuous casting process parameters on CSI were investigated based on the predictions of the R-ELM model via response surface analysis. The conclusions are consistent with the metallurgical mechanism, and the predictive values of the R-ELM model agree well with experimental values, which further verifies the correctness and generalization ability of the R-ELM model.
A Novel GaN Metal-Insulator-Semiconductor High Electron Mobility Transistor Featuring Vertical Gate Structure
Authors: Sun Zhonghao , Huang Huolin , Sun Nan , Tao Pengcheng , Zhao Cezhou , Liang Yung C.
Published: December 2019

A novel structure scheme by transposing the gate channel orientation from a long horizontal one to a short vertical one is proposed and verified by technology computer-aided design (TCAD) simulations to achieve GaN-based normally-off high electron mobility transistors (HEMTs) with reduced on-resistance and improved threshold voltage. The proposed devices exhibit high threshold voltage of 3.1 V, high peak transconductance of 213 mS, and much lower on-resistance of 0.53 mΩ·cm2 while displaying better off-state characteristics owing to more uniform electric field distribution around the recessed gate edge in comparison to the conventional lateral HEMTs. The proposed scheme provides a new technical approach to realize high-performance normally-off HEMTs.
Development and Evaluation of a Duo Zaire ebolavirus Real-Time RT-PCR Assay Targeting Two Regions within the Genome
Authors: Thirion Laurence , Charrel Remi N. , Boehmann Yannik , Corcostegui Iban , Raoul Hervé , de Lamballerie Xavier
Published: December 2019

Preparedness and response actions to mitigate Ebola virus disease (EVD) outbreaks rely on rapid diagnosis to be implemented locally to sort suspect patients attending health centers. Our aim was (i) to develop and evaluate an RT-qPCR assay combining primers and probes derived from two reference assays targeting different genomic regions; (ii) to study whether sensitivity and specificity of this dual-target assay were at least equal or better to the parental assays; (iii) to implement this dual-target assay onto the Cepheid GeneXpert open cartridge as a proof of principle for technological transfer aiming at bedsite testing locally. To do so, three home-made published RT-qPCR assays were selected to be compared with the RealStar® Filovirus Screen RT-PCR kit 1.0 (Altona Diagnostics, Hamburg, Germany), a technique that was largely deployed during the 2014–2015 West African EVD outbreak. Primers and probes sequences of the custom-made assays were analyzed in silico against a multiple sequence alignment, including >250 complete sequences corresponding to strains that have caused EVD epidemics in the past. Genomic RNA purified from the Mekambo strain of Zaire ebolavirus (EBOV) was used to study the sensitivity of the five methods. Based on these results, two in-house methods were selected and adapted to design the dual-target assay, which performances were compared to those of the parental assays using a synthetic RNA control. The dual-target assay showed better sensitivity and limit of detection (LoD95 at 0.4 copies/µL) than the parental methods (1.7 and 2.2 copies/µL). Ultimately, the dual-target assay was transferred onto the GeneXpert Flex-03 open cartridge, demonstrating a LoD95 at 0.75 copies/µL. Together these results indicate that EBOV dual-target assay has the potential to be used during EVD outbreak in the laboratory having performed molecular testing during the recent outbreaks.
Carotenoid-Producing Yeasts: Identification and Characteristics of Environmental Isolates with a Valuable Extracellular Enzymatic Activity
Authors: Chreptowicz Karolina , Mierzejewska Jolanta , Tkáčová Jana , Młynek Mateusz , Čertik Milan
Published: December 2019

Sixteen cold-adapted reddish-pigmented yeast strains were obtained from environmental samples. According to the PCR-based detection of classical yeast markers combined with phylogenetic studies, the yeasts belong mainly to the genera Rhodotorula, Sporobolomyces and Cystobasidium, all within the subphylum Pucciniomycotina. All strains produced carotenoids within a 0.25–10.33 mg/L range under non-optimized conditions. Noteworthily, among them, representatives of the Cystobasidium genus were found; of particular value are the strains C. laryngis and C. psychroaquaticum, poorly described in the literature to date. Interestingly, carotenoid production with representatives of Cystobasidium was improved 1.8- to 10-fold at reduced temperature. As expected, most of the isolated yeasts biosynthesized extracellular lipases, but within them also one proteolytic and four cellulolytic strains were revealed. We succeeded in isolating strain Cystofilobasidium macerans WUT145 with extraordinarily high cellulolytic activity at 22°C (66.23 ± 0.15 µmol/mg protein·min) that is described here for the first time. Consequently, a set of yeasts capable of producing both carotenoids and extracellular enzymes was identified. Taking into account those abilities, the strains might be applicable for a development of carotenoids production on an agro-industrial waste, e.g., lignocellulose.