bluuebunny/crossref_metadata_2025 huggingface dataset:
Cerebro-Cerebellar Networks in Migraine Symptoms and Headache
Authors: Noseda Rodrigo
Published: July 2022

The cerebellum is associated with the biology of migraine in a variety of ways. Clinically, symptoms such as fatigue, motor weakness, vertigo, dizziness, difficulty concentrating and finding words, nausea, and visual disturbances are common in different types of migraine. The neural basis of these symptoms is complex, not completely known, and likely involve activation of both specific and shared circuits throughout the brain. Posterior circulation stroke, or neurosurgical removal of posterior fossa tumors, as well as anatomical tract tracing in animals, provided the first insights to theorize about cerebellar functions. Nowadays, with the addition of functional imaging, much progress has been done on cerebellar structure and function in health and disease, and, as a consequence, the theories refined. Accordingly, the cerebellum may be useful but not necessary for the execution of motor, sensory or cognitive tasks, but, rather, would participate as an efficiency facilitator of neurologic functions by improving speed and skill in performance of tasks produced by the cerebral area to which it is reciprocally connected. At the subcortical level, critical regions in these processes are the basal ganglia and thalamic nuclei. Altogether, a modulatory role of the cerebellum over multiple brain regions appears compelling, mainly by considering the complexity of its reciprocal connections to common neural networks involved in motor, vestibular, cognitive, affective, sensory, and autonomic processing—all functions affected at different phases and degrees across the migraine spectrum. Despite the many associations between cerebellum and migraine, it is not known whether this structure contributes to migraine initiation, symptoms generation or headache. Specific cerebellar dysfunction via genetically driven excitatory/inhibitory imbalances, oligemia and/or increased risk to white matter lesions has been proposed as a critical contributor to migraine pathogenesis. Therefore, given that neural projections and functions of many brainstem, midbrain and forebrain areas are shared between the cerebellum and migraine trigeminovascular pathways, this review will provide a synopsis on cerebellar structure and function, its role in trigeminal pain, and an updated overview of relevant clinical and preclinical literature on the potential role of cerebellar networks in migraine pathophysiology.
High-Energy Enteral Nutrition in Infants After Complex Congenital Heart Surgery
Authors: Ni Ping , Chen Xi , Zhang Yueyue , Zhang Mingjie , Xu Zhuoming , Luo Wenyi
Published: July 2022

Background

Malnutrition is common in complex congenital heart disease (CCHD). The purpose of this study was to compare the safety and efficacy of early initiation of high-energy enteral nutrition (EN) with regular energy EN in infants after surgery for CCHD.

Methods

This is a subgroup analysis of a randomized controlled trial (RCT) which was conducted in the cardiac intensive care unit (CICU) of the largest pediatric heart center in China. Eighty children with CCHD after surgery were from two groups, the intervention group (n = 40) was given high-energy EN and the control group (n = 40) was given regular energy EN. We analyzed the effects of the two interventions on outcomes such as caloric attainment rate, gastrointestinal intolerance, duration of mechanical ventilation, and anthropometry at discharge.

Results

There was no difference in the daily milk intake between the two groups, but the calorie intake (50.2 vs. 33.4, P < 0.001), protein intake (1.1 vs. 0.9, P < 0.001) and caloric attainment rate were higher in the intervention group (77.5 vs. 45.0%, P = 0.003). In addition, the incidence of pneumonia (P = 0.003) and duration of mechanical ventilation (P = 0.008) were less in the intervention group, and biceps circumference and triceps skinfold thickness at hospital discharge were greater than those in the control group (P < 0.001). We have not found statistical differences in gastrointestinal intolerance, glycemic fluctuations, incidence of pressure ulcers, length of CICU stay and postoperative hospital days between the two groups.

Conclusions

Early initiation of high-energy EN may be safe and effective in infants after complex cardiac surgery. Low doses high-energy EN did not increase gastrointestinal intolerance or glycemic fluctuations and also improved post-operative nutrition by increasing caloric and protein intake without increasing fluid intake.
Case Report: Intermittent Chest Pain With Cough, Fever, and Pericardial Effusion Over the Course of 1 Year in an 11-Year-Old Girl
Authors: Chen Jing , Qin Lu , Tang Lan-Fang
Published: July 2022

An 11-year-old girl presented with frequent chest pain, fever, and a cough that she had had for more than 13 months, as well as pleural effusion and large pericardial effusion. She was misdiagnosed with tuberculosis and received anti-tuberculosis drugs for 6 months. Within the past year, she also underwent two more thoracotomies and a thoracoscopic partial pericardiectomy. The final diagnosis of pulmonary paragonimiasis was established once it was known that she had eosinophilia, always drank stream water, and tested positive for antibodies against Paragonimus. Since antiparasitic praziquantel therapy was effective, paragonimiasis should be considered as a possibility in the differential diagnosis of tuberculosis in children.
Vitamin D Supplementation: Association With Serum Cytokines in Pediatric Hematopoietic Stem Cell Transplantation
Authors: Olsen Braden , Bodea Jessica , Garcia Angela , Beebe Kristen , Campbell Courtney , Schwalbach Carly , Salzberg Dana , Miller Holly , Adams Roberta , Mirea Lucia , Castillo Paul , Horn Biljana , Bansal Sandhya , Mohanakumar Thalachallour , Ngwube Alexander
Published: July 2022

Vitamin D deficiency is prevalent in pediatric patients presenting for hematopoietic stem cell transplantation (HSCT) and has been linked to poor clinical outcomes. Using the data from a randomized control trial, in this paper we explore the effects of vitamin D supplementation on circulating cytokine levels during pediatric HSCT (www.clinicaltrials.gov as NCT03176849). A total of 41 children, 20 received Stoss therapy and 21 children received standard of care vitamin D supplementation. Levels of 25(OH)D and 20 cytokines were assessed at baseline and day +30. Significantly (P < 0.05) higher levels of mostly proinflammatory cytokines, FGF, GCSF, TNFα, IL-2, IL-6, IP10 were detected pre-transplant for patients with low compared to those with normal vitamin D levels. In sex stratified models that compare changes in cytokines between Stoss vs. standard of care, females in the Stoss group show greater changes in mostly pro -inflammatory cytokines- IP-10 (P = 0.0047), MIG (P = 0.009), and RANTES (P = 0.0047), IL-2R (P = 0.07) and IL-6(P = 0.069). Despite a small sample size, these findings suggest vitamin D deficiency affects the pre-transplant cytokine milieu and higher doses of vitamin D (Stoss therapy) appears to influence proinflammatory cytokine responses in a sex specific manner during pediatric HSCT. Larger clinical trials are warranted to validate these results.
A Case-Based Monitoring Approach to Evaluate Safety of COVID-19 Vaccines in a Partially Integrated Health Information System: A Study Protocol
Authors: Ab Rahman Norazida , Lim Ming Tsuey , Lee Fei Yee , Ong Su Miin , Peariasamy Kalaiarasu M. , Sivasampu Sheamini
Published: July 2022

In response to Coronavirus disease 2019 (COVID-19) global pandemic, various COVID-19 vaccines were rapidly administered under emergency use authorization. Rare outcomes associated with COVID-19 vaccines might be less likely to be captured in clinical trials, leading to a knowledge gap in real-world vaccine safety. In contrast with high-income countries, many low-to-middle income countries have limited capacity to conduct active surveillance, owing to the absence of large and fully-integrated health information databases. This paper describes the study protocol, which aims to investigate risk of prespecified adverse events of special interests following COVID-19 vaccination in a partially integrated health information system with non-shareable electronic health records. The SAFECOVAC study is a longitudinal, observational retrospective study of active safety surveillance using case-based monitoring approach. This involves linkage of several administrative databases and hospitalization data monitoring to identify adverse events of special interests following administration of COVID-19 vaccines in Malaysia. The source population comprises of all individuals who received at least one dose of COVID-19 vaccine. Self-controlled design and vaccinated case-coverage design will be employed to assess risk of adverse events of special interests and determine the association with vaccine exposure. Data on vaccination records will be obtained from the national COVID-19 vaccination register to identify the vaccination platforms, doses and the timing of vaccinations. The outcome of this study is hospitalization for the adverse events of special interests between March 2021 and June 2022. The outcomes will be obtained through linkage with hospital admission database and national pharmacovigilance database. Findings will provide analysis of real-world data which can inform deliberations by government and public health decision makers relative to the refinement of COVID-19 vaccination recommendations.
ACSL4 as a Potential Target and Biomarker for Anticancer: From Molecular Mechanisms to Clinical Therapeutics
Authors: Hou Jun , Jiang Changqing , Wen Xudong , Li Chengming , Xiong Shiqiang , Yue Tian , Long Pan , Shi Jianyou , Zhang Zhen
Published: July 2022

Cancer is a major public health problem around the world and the key leading cause of death in the world. It is well-known that glucolipid metabolism, immunoreaction, and growth/death pattern of cancer cells are markedly different from normal cells. Recently, acyl-CoA synthetase long-chain family 4 (ACSL4) is found be participated in the activation of long chain fatty acids metabolism, immune signaling transduction, and ferroptosis, which can be a promising potential target and biomarker for anticancer. Specifically, ACSL4 inhibits the progress of lung cancer, estrogen receptor (ER) positive breast cancer, cervical cancer and the up-regulation of ACSL4 can improve the sensitivity of cancer cells to ferroptosis by enhancing the accumulation of lipid peroxidation products and lethal reactive oxygen species (ROS). However, it is undeniable that the high expression of ACSL4 in ER negative breast cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer can also be related with tumor cell proliferation, migration, and invasion. In the present review, we provide an update on understanding the controversial roles of ACSL4 in different cancer cells.
Dynamics of Rotating Spin-Orbit-Coupled Spin-1 Bose-Einstein Condensates With In-Plane Gradient Magnetic Field in an Anharmonic Trap
Authors: Yang Hui , Zhang Qing , Jian Zaihe
Published: July 2022

We investigate the dynamics of rotating spin-orbit-coupled spin-1 Bose–Einstein condensates (BECs) in an in-plane gradient magnetic field, which is confined in an anharmonic trap. In the case of rotating spin-orbit-coupled spin-1 BECs with given parameters, the system evolves from initial disk-shaped condensates into drastic turbulent oscillations and ghost vortices on the surface of the component densities due to surface wave excitations and then into two final vortex necklaces along the azimuthal direction with an irregular density hole, in which the vortices differ by one quantum number in turn. For the case of rotating spin-orbit-coupled spin-1 BECs with in-plane gradient magnetic field, with the dynamic evolution, the system undergoes a transition from an initial central polar-core vortex to violent turbulent oscillations and then to a final vortex chain along the diagonal of BECs, with the remaining vortices symmetrically distributed on both sides in the individual component. In addition, the corresponding spin texture undergoes a transition from plane-wave phase to double half-antiskyrmion necklaces for the former case and a transition from a structure similar to a quadrupole magnetic field to a half-antiskyrmion chain with the rest of the half-antiskyrmions on both sides. During the dynamic evolution process, the angular momentum increases gradually and then approaches a convergent value.
A Spectral Principal Component Analysis-Based Framework for Composite Hard/Soft Tissue Fluorescence Image Investigation
Authors: Piriou Marie , Lorenzo Corinne , Raymond-Letron Isabelle , Coronas-Dupuis Sophie , Pieruccioni Laetitia , Rouquette Jacques , Guissard Christophe , Chaumont Jade , Casteilla Louis , Planat-Benard Valérie , Kemoun Philippe , Monsarrat Paul
Published: July 2022

Traditional thin sectioning microscopy of large bone and dental tissue samples using demineralization may disrupt structure morphologies and even damage soft tissues, thus compromising the histopathological investigation. Here, we developed a synergistic and original framework on thick sections based on wide-field multi-fluorescence imaging and spectral Principal Component Analysis (sPCA) as an alternative, fast, versatile, and reliable solution, suitable for highly mineralized tissue structure sustain and visualization. Periodontal 2-mm thick sections were stained with a solution containing five fluorescent dyes chosen for their ability to discriminate close tissues, and acquisitions were performed with a multi-zoom macroscope for blue, green, red, and NIR (near-infrared) emissions. Eigen-images derived from both standard scaler (Std) and Contrast Limited Adaptive Histogram Equalization (Clahe) pre-preprocessing significantly enhanced tissue contrasts, highly suitable for histopathological investigation with an in-depth detail for sub-tissue structure discrimination. Using this method, it is possible to preserve and delineate accurately the different anatomical/morphological features of the periodontium, a complex tooth-supporting multi-tissue. Indeed, we achieve characterization of gingiva, alveolar bone, cementum, and periodontal ligament tissues. The ease and adaptability of this approach make it an effective method for providing high-contrast features that are not usually available in standard staining histology. Beyond periodontal investigations, this first proof of concept of an sPCA solution for optical microscopy of complex structures, especially including mineralized tissues opens new perspectives to deal with other chronic diseases involving complex tissue and organ defects. Overall, such an imaging framework appears to be a novel and convenient strategy for optical microscopy investigation.
Fractal Structure of Brain Electrical Activity of Patients With Mental Disorders
Authors: E Dick O. , V Murav’eva S. , S Lebedev V. , E Shelepin Yu.
Published: July 2022

This work was aimed at a comparative analysis of the degree of multifractality of electroencephalographic time series obtained from a group of healthy subjects and from patients with mental disorders. We analyzed long-term records of patients with paranoid schizophrenia and patients with depression. To evaluate the properties of multifractal scaling of various electroencephalographic time series, the method of maximum modulus of the wavelet transform and multifractal analysis of fluctuations without a trend were used. The stability of the width and position of the singularity spectrum for each of the test groups was revealed, and a relationship was established between the correlation and anticorrelation dynamics of successive values of the electroencephalographic time series and the type of mental disorders. It was shown that the main differences between the multifractal properties of brain activity in normal and pathological conditions lie in the different width of the multifractality spectrum and its location associated with the correlated or anticorrelated dynamics of the values of successive time series. It was found that the schizophrenia group is characterized by a greater degree of multifractality compared to the depression group. Thus, the degree of multifractality can be included in a set of tests for differential diagnosis and research of mental disorders.
Plasma Levels of Acyl-Carnitines and Carboxylic Acids Correlate With Cardiovascular and Kidney Function in Subjects With Sickle Cell Trait
Authors: Nemkov Travis , Skinner Sarah , Diaw Mor , Diop Saliou , Samb Abdoulaye , Connes Philippe , D’Alessandro Angelo
Published: July 2022

Subjects with sickle cell trait (SCT) carry one copy of mutated β-globin gene at position E6V at the origin of the production of sickle hemoglobin (HbS). Indeed, individuals with SCT have both normal hemoglobin and HbS, in contrast to patients with sickle cell disease who inherited of two copies of the mutated gene. Although SCT is generally benign/asymptomatic, carriers may develop certain adverse outcomes such as renal complications, venous thromboembolism, exercise-induced rhabdomyolysis … However, little is known about whether similar metabolic pathways are affected in individuals with SCT and whether these metabolic derangements, if present, correlate to clinically relevant parameters. In this study, we performed metabolomics analysis of plasma from individuals with sickle cell trait (n = 34) compared to healthy controls (n = 30). Results indicated a significant increase in basal circulating levels of hemolysis markers, mono- (pyruvate, lactate), di- and tri-carboxylates (including all Krebs cycle intermediates), suggestive of systems-wide mitochondrial dysfunction in individuals with SCT. Elevated levels of kynurenines and indoles were observed in SCT samples, along with increases in the levels of oxidative stress markers (advanced glycation and protein-oxidation end-products, malondialdehyde, oxylipins, eicosanoids). Increases in circulating levels of acyl-carnitines and fatty acids were observed, consistent with increased membrane lipid damage in individuals with sickle cell trait. Finally, correlation analyses to clinical co-variates showed that alterations in the aforementioned pathways strongly correlated with clinical measurements of blood viscosity, renal (glomerular filtration rate, microalbuminuria, uremia) and cardiovascular function (carotid-femoral pulse wave velocity, blood pressure).
Different Types of Visual Perturbation Induced Different Demands and Patterns in Active Control: Implication for Future Sensorimotor Training
Authors: Ren Muchen , Lin Tangdi , Chien Jung Hung
Published: July 2022

Background: Sensorimotor training using visual perturbations has been widely applied to astronauts for rapidly handling and adapting to unpredictable environments. However, these visual perturbations might not be strong enough to trigger long-term effects. Therefore, this study aimed to develop a novel sensorimotor training paradigm using pseudo-random visual perturbations and to determine the demands and patterns of active control under different types of visual perturbations.

Method: Thirty healthy young adults participated in this study. Four walking conditions were randomly assigned to these participants: 1) walking without optic flow (NoOptic), 2) walking with the optic flow (Optic), 3) walking under reduced visual capability (Vre), and 4) walking under perturbed optic flow (Vpe). The dependent variables were the step length variability, the step width variability, the 95% confidence interval ellipse area, the long axis of the ellipse, and the short axis of the ellipse.

Results: The results indicated that 1) the step length variability and the ellipse area were greater in Vre compared to Optic (p < 0.001, p < 0.001). Moreover, the step width variability and ellipse area were greater in Vpe than Optic (p < 0.001, p = 0.002).

Conclusion: The abovementioned results demonstrated that 1) walking in both Vre and Vpe conditions required greater demands and different patterns in active controls compared to the Optic condition, suggesting both Vre and Vpe conditions could be applied for sensorimotor training; 2) the Vre condition would be the first choice if there were no concerns in potential trips on the treadmill.
Long-Term Inhalation of Ultrafine Zinc Particles Deteriorated Cardiac and Cardiovascular Functions in Rats of Myocardial Infarction
Authors: Huo Yunlong , Li Li
Published: July 2022

Substantial ultrafine zinc particles exist in air pollutions. The level of Zn concentrations in serum and tissue could affect patients with myocardial infarction (MI). The aim of the study is to investigate the change of cardiac functions and peripheral hemodynamics in MI rats after long-term inhalation of ultrafine Zn particles. Coronary artery ligation surgery was performed to induce MI in Wistar rats. The inhalation of ultrafine Zn particles was carried out for 6 weeks after the operation. Physiological and hemodynamic measurements and computational biomechanics analysis were demonstrated in eight groups of rats at postoperative 4 and 6 weeks. There was no statistical significance between shams and shams with inhalation of ultrafine Zn particles. There were significant impairments of cardiac and hemodynamic functions in MI rats. In comparison with MI rats, the inhalation of ultrafine Zn particles for 4 weeks slowed down the progression from MI to heart failure, but the inhalation for 6 weeks accelerated the process. The long-term inhalation of ultrafine zinc particles induced excessive accumulation of zinc in serum and tissue, which deteriorated cardiac and hemodynamic dysfunctions in MI rats. The findings suggested the importance for regulating Zn intake of MI patients as well as looking at ways to lower zinc concentrations in air pollutions.
Treatment of Cardiovascular Dysfunction with PDE3-Inhibitors in Moderate and Severe Hypothermia—Effects on Cellular Elimination of Cyclic Adenosine Monophosphate and Cyclic Guanosine Monophosphate
Authors: Kuzmiszyn Adrina Kalasho , Selli Anders Lund , Smaglyukova Natalia , Kondratiev Timofei , Fuskevåg Ole-Martin , Lyså Roy Andre , Ravna Aina Westrheim , Tveita Torkjel , Sager Georg , Dietrichs Erik Sveberg
Published: July 2022

Introduction: Rewarming from accidental hypothermia is often complicated by hypothermia-induced cardiovascular dysfunction, which could lead to shock. Current guidelines do not recommend any pharmacological treatment at core temperatures below 30°C, due to lack of knowledge. However, previous in vivo studies have shown promising results when using phosphodiesterase 3 (PDE3) inhibitors, which possess the combined effects of supporting cardiac function and alleviating the peripheral vascular resistance through changes in cyclic nucleotide levels. This study therefore aims to investigate whether PDE3 inhibitors milrinone, amrinone, and levosimendan are able to modulate cyclic nucleotide regulation in hypothermic settings.

Materials and methods: The effect of PDE3 inhibitors were studied by using recombinant phosphodiesterase enzymes and inverted erythrocyte membranes at six different temperatures—37°C, 34°C, 32°C, 28°C, 24°C, and 20°C- in order to evaluate the degree of enzymatic degradation, as well as measuring cellular efflux of both cAMP and cGMP. The resulting dose-response curves at every temperature were used to calculate IC50 and Ki values.

Results: Milrinone IC50 and Ki values for cGMP efflux were significantly lower at 24°C (IC50: 8.62 ± 2.69 µM) and 20°C (IC50: 7.35 ± 3.51 µM), compared to 37°C (IC50: 22.84 ± 1.52 µM). There were no significant changes in IC50 and Ki values for enzymatic breakdown of cAMP and cGMP.

Conclusion: Milrinone, amrinone and levosimendan, were all able to suppress enzymatic degradation and inhibit extrusion of cGMP and cAMP below 30°C. Our results show that these drugs have preserved effect on their target molecules during hypothermia, indicating that they could provide an important treatment option for hypothermia-induced cardiac dysfunction.
Neurochemical Signalling Associated With Gill Oxygen Sensing and Ventilation: A Receptor Focused Mini-Review
Authors: Reed Maddison , Jonz Michael G.
Published: July 2022

Despite the large body of work describing vertebrate ventilatory responses to hypoxia, remarkably little is known about the receptors and afferent pathways mediating these responses in fishes. In this review, we aim to summarize all receptor types to date implicated in the neurotransmission or neuromodulation associated with O2 sensing in the gills of fish. This includes serotonergic, cholinergic, purinergic, and dopaminergic receptor subtypes. Recent transcriptomic analysis of the gills of zebrafish using single-cell RNA sequencing has begun to elucidate specific receptor targets in the gill; however, the absence of receptor characterization at the cellular level in the gill remains a major limitation in understanding the neurochemical control of hypoxia signalling.
Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease
Authors: Hoffmann Lucia Vieira , Branquinho Amanda Alves , Barroso Paulo Augusto Vianna , Vaslin Maite F. S.
Published: July 2022

The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (α-GQE, α-PRN, and α-INK) and monoclonal (α-GQE, α-PRN, and α-NKF) antibodies. All six were tested as capture antibodies, and polyclonal α-GQE and the monocle onal α-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.
Bambara Groundnut Rhizobacteria Antimicrobial and Biofertilization Potential
Authors: F. Ajilogba Caroline , Babalola Olubukola O. , Adebola Patrick , Adeleke Rasheed
Published: July 2022

Bambara groundnut, an underutilized crop has been proved to be an indigenous crop in Africa with the potential for food security. The rhizosphere of Bambara groundnut contains Rhizobacteria, with the ability to grow, adapt, and colonize their surroundings even in unfavorable conditions and have not been explored for their plant growth-promoting properties. The aim of this research was to determine the potential of rhizobacteria from Bambara groundnut soil samples as either biofertilizers or biocontrol agents or both to help provide sustainable agriculture in Africa and globally. Bambara groundnut rhizospheric soil samples were collected and analyzed for their chemical composition. Rhizobacteria isolates were cultured from the soil samples. Plant growth-promoting, antifungal activities and phylogenetic analysis using 16S rRNA were carried out on the isolates to identify the rhizobacteria. A 2-year field study planting was carried out to determine the effect of these rhizobacteria as biofertilizers for Bambara groundnut (Vigna subterranean). The study was carried out in a complete randomized block experimental design with three replications. All the isolates were able to produce ammonia and 1-aminocyclopropane-1-carboxylate, while 4.65, 12.28, and 27.91% produced hydrogen cyanide, indole acetic acid, and solubilized phosphate, respectively, making them important targets as biocontrol and biofertilizer agents. The field results revealed that treatment with rhizobacteria had significant results compared with the control. Characterization of selected isolates reveals their identity as B. amyloliquefaciens, B. thuringiensis, and Bacillus sp. These Bacillus isolates have proved to be plant growth-promoting agents that can be used as biofertilizers to enhance the growth of crops and consequent improved yield. This is the first time the rhizobacteria from the Bambara groundnut rhizosphere are applied as biofertilizer.
Transcriptomic Complexity in Strawberry Fruit Development and Maturation Revealed by Nanopore Sequencing
Authors: Chen Qing , Lin Ximeng , Tang Wenlu , Deng Qian , Wang Yan , Lin Yuanxiu , He Wen , Zhang Yunting , Li Mengyao , Luo Ya , Zhang Yong , Wang Xiaorong , Tang Haoru
Published: July 2022

The use of alternative transcription start or termination sites (aTSS or aTTS) as well as alternative splicing (AS) produce diverse transcript isoforms, playing indispensable roles in the plant development and environmental adaptations. Despite the advances in the finding of the genome-wide alternatively spliced genes in strawberry, it remains unexplored how AS responds to the developmental cues and what relevance do these outcomes have to the gene function. In this study, we have systematically investigated the transcriptome complexity using long-read Oxford Nanopore Technologies along the four successive developmental stages. The full-length cDNA sequencing results unraveled thousands of previously unexplored transcript isoforms raised from aTSS, aTTS, and AS. The relative contributions of these three processes to the complexity of strawberry fruit transcripts were compared. The aTSS and aTTS were more abundant than the AS. Differentially expressed transcripts unraveled the key transitional role of the white fruit stage. Isoform switches of transcripts from 757 genes were observed. They were associated with protein-coding potential change and domain gain or loss as the main consequences. Those genes with switched isoforms take part in the key processes of maturation in the late stages. A case study using yeast two hybrid analysis supported the functional divergence of the two isoforms of the B-box protein 22. Our results provided a new comprehensive overview of the dynamic transcriptomic landscape during strawberry fruit development and maturation.
Melatonin Mediates Axillary Bud Outgrowth by Improving Nitrogen Assimilation and Transport in Rice
Authors: Yang Guo , Wei Xilin , Fang Zhongming
Published: July 2022

Melatonin plays an important role in plant resistance to biotic and abiotic stresses. However, whether melatonin is involved in the regulation of plant architecture, such as the formation of axillary bud outgrowth or tillering, in rice remains unknown. Here, we found that different concentrations of melatonin influenced axillary bud outgrowth in rice, and moderate melatonin concentrations also alleviated the inhibition of axillary bud outgrowth in the presence of high concentrations of basic amino acids lysine and arginine. Furthermore, transcriptome analysis demonstrated that genes involved in nitrogen metabolism and phytohormone signal transduction pathways may affect axillary bud outgrowth, which is regulated by melatonin. We determined that the differentially expressed genes glutamine synthetase OsGS2 and amino acid transporter OsAAP14, which are involved in nitrogen metabolism and are regulated by melatonin and basic amino acids, were the key regulators of axillary bud outgrowth in rice. In addition, we validated the functions of OsGS2 and OsAAP14 using rice transgenic plants with altered axillary bud outgrowth and tillers. Taken together, these results suggest that melatonin mediates axillary bud outgrowth by improving nitrogen assimilation and transport in rice.
Exploring Soybean Flower and Pod Variation Patterns During Reproductive Period Based on Fusion Deep Learning
Authors: Zhu Rongsheng , Wang Xueying , Yan Zhuangzhuang , Qiao Yinglin , Tian Huilin , Hu Zhenbang , Zhang Zhanguo , Li Yang , Zhao Hongjie , Xin Dawei , Chen Qingshan
Published: July 2022

The soybean flower and the pod drop are important factors in soybean yield, and the use of computer vision techniques to obtain the phenotypes of flowers and pods in bulk, as well as in a quick and accurate manner, is a key aspect of the study of the soybean flower and pod drop rate (PDR). This paper compared a variety of deep learning algorithms for identifying and counting soybean flowers and pods, and found that the Faster R-CNN model had the best performance. Furthermore, the Faster R-CNN model was further improved and optimized based on the characteristics of soybean flowers and pods. The accuracy of the final model for identifying flowers and pods was increased to 94.36 and 91%, respectively. Afterward, a fusion model for soybean flower and pod recognition and counting was proposed based on the Faster R-CNN model, where the coefficient of determinationR2 between counts of soybean flowers and pods by the fusion model and manual counts reached 0.965 and 0.98, respectively. The above results show that the fusion model is a robust recognition and counting algorithm that can reduce labor intensity and improve efficiency. Its application will greatly facilitate the study of the variable patterns of soybean flowers and pods during the reproductive period. Finally, based on the fusion model, we explored the variable patterns of soybean flowers and pods during the reproductive period, the spatial distribution patterns of soybean flowers and pods, and soybean flower and pod drop patterns.
Exploring the Integration of Engineering Design Practices in Tenth-Grade Chemistry Activities
Authors: Aldahmash Abdulwali H. , Alfarraj Yousef F.
Published: July 2022

Chemistry textbooks are the most popular teaching material in schools. They can contribute significantly to the attainment of scientific educational goals. Internationally, educational reforms in science subjects are adopting newer practices such as Engineering Design Processes (EDP) for addressing real-world requirements. This study, conducted in Saudi Arabia, employed a qualitative and quantitative content analysis method to evaluate the level of the EDP incorporated in the tenth grade chemistry textbook and accompanying student’s experiments’ guidebook. The results found the inclusive mean for EDP was 1.05, which indicated incorporation was found to be at level one. This inclusion EDP in chemistry textbooks has been rated as novice or deficient, which indicates that it does not fulfill the majority of the requirements for inclusion as suggested by NGSS.
Motivated Down-Regulation of Emotion and Compassion Collapse Revisited
Authors: Hagman William , Tinghög Gustav , Dickert Stephan , Slovic Paul , Västfjäll Daniel
Published: July 2022

Compassion collapse is a phenomenon where feelings and helping behavior decrease as the number of needy increases. But what are the underlying mechanisms for compassion collapse? Previous research has attempted to pit two explanations: Limitations of the feeling system vs. motivated down-regulation of emotion, against each other. In this article, we critically reexamine a previous study comparing these two accounts published in 2011 and present new data that contest motivated down-regulation of emotion as the primary explanation for compassion collapse.
A Study on Multimedia Integrated Pre-service Education to Learning Behavior and Competitiveness in Workplace of Employees in Hospitality
Authors: Pai Chih-Hung , Wang Yu-Lan , Shang Yunfeng , Hsu Ta-Kuang
Published: July 2022

The domestic situation of the past few years shows the practices of employees’ unpaid leave and layoffs and the constant drain on capital, talent, and technologies in hospitality. Owners expect to reduce the losses to as low as possible by saving on human costs. Nevertheless, in face of such a changing environment, hospitality has to accumulate high-quality human capital through systematic investment, sensitive development, and continuous learning and growth to discover competitive advantages through the cultivation of human capital. The pre-service education of new employees could accelerate their familiarity with the operations of the company and their understanding of their job role and duties. More importantly, with good planning, it could make employees feel emphasized with and respected with the result of largely changing their thoughts and working habits. Aiming at supervisors and employees in hospitality in Zhejiang as the research objects, a total of 420 copies of our questionnaire are distributed, and 357 valid copies were retrieved, with a retrieval rate of 85%. According to the results to propose discussions, it is expected to generate systematic and proper education methods for the pre-service education in hospitality, promote the education effectiveness, and promote employees’ capability and organizational performance.
Research on the Method of Depression Detection by Single-Channel Electroencephalography Sensor
Authors: Lei Xue , Ji Weidong , Guo Jingzhou , Wu Xiaoyue , Wang Huilin , Zhu Lina , Chen Liang
Published: July 2022

Depression is a common mental health illness worldwide that affects our quality of life and ability to work. Although prior research has used EEG signals to increase the accuracy to identify depression, the rates of underdiagnosis remain high, and novel methods are required to identify depression. In this study, we built a model based on single-channel, dry-electrode EEG sensor technology to detect state depression, which measures the intensity of depressive feelings and cognitions at a particular time. To test the accuracy of our model, we compared the results of our model with other commonly used methods for depression diagnosis, including the PHQ-9, Hamilton Depression Rating Scale (HAM-D), and House-Tree-Person (HTP) drawing test, in three different studies. In study 1, we compared the results of our model with PHQ-9 in a sample of 158 senior high students. The results showed that the consistency rate of the two methods was 61.4%. In study 2, the results of our model were compared with HAM-D among 71 adults. We found that the consistency rate of state-depression identification by the two methods was 63.38% when a HAM-D score above 7 was considered depression, while the consistency rate increased to 83.10% when subjects showed at least one depressive symptom (including depressed mood, guilt, suicide, lack of interest, retardation). In study 3, 68 adults participated in the study, and the results revealed that the consistency rate of our model and HTP drawing test was 91.2%. The results showed that our model is an effective means to identify state depression. Our study demonstrates that using our model, people with state depression could be identified in a timely manner and receive interventions or treatments, which may be helpful for the early detection of depression.
Is the Processing of Chinese Verbal Metaphors Simulated or Abstracted? Evidence From an ERP Study
Authors: Li Ying , Lu Xiaoxiao , Wang Yizhen , Wang Hanlin , Wang Yue
Published: July 2022

The theory of embodied semantics holds that verbal metaphors are strongly grounded in sensorimotor experience. Many studies have proven that besides sensorimotor simulation, the comprehension of verbal metaphors also requires semantic abstraction. But the interaction between simulation and abstraction, as well as the time course of metaphorical meaning integration, is not well understood. In the present study, we aimed to investigate whether embodiment or abstraction, or both, is employed in the processing of Chinese verbal metaphor. Participants were asked to read subject-verb metaphorical, verb-object metaphorical, literal-concrete and literal-abstract sentences, and the target words were measured at the verb and the object of each sentence. The results revealed that a similar N400 effect was elicited by the target verbs in the verb-object metaphorical and the literal-concrete sentences, and a similar P600/LPC effect was induced by the target verbs in the subject-verb metaphorical and the literal-abstract sentences, reflecting that the verb-object metaphors trigger a simulation process, while the subject-verb metaphors trigger an abstraction process in the verb processing stage. Moreover, the subject-verb metaphors elicited a stronger P600/LPC effect by the target verbs than the verb-object metaphors, but there was no difference of the P600/LPC caused by the target objects between the two kinds of metaphors, revealing that the metaphorical meaning of a subject-verb metaphor is integrated in the verb processing stage, while that of a verb-object metaphor is reanalyzed in the object processing stage. These results suggest that a verbal metaphor is processed both by simulation and abstraction, and the metaphorical meaning is integrated immediately with the unfolding of the sentence meaning. The position where the semantic conflict lies in a sentence (verb vs. object) modulates the time course of metaphor sentence comprehension.
CFO Gender, Corporate Risk-Taking, and Information Disclosure Violations
Authors: Zhao Yujie , Xiong Jiaxin , Wang Jingjing , Ye Nanji
Published: July 2022

The sex ratio at birth in China exhibits a major occurrence of “missing women” due to the high son preference in Chinese culture. Clearly, the large gender discrepancy in China can be explained not only by ethical, moral, or social fairness theories but also by the economic benefits of women's particular abilities, experiences, and talents. This article examines the influence of female chief financial officers (CFOs) on information disclosure violations in order to highlight women's positive contributions. Our data imply that having a female CFO can dramatically lower the number of companies that fail to disclose information. The results are strong after controlling endogeneity with propensity score matching, Heckman's two-stage self-selection model, and CFO change, as well as controlling the gender of the chairman and chief executive officer, utilizing different study periods, and using exogenous shock. We further examined the moderate effects of CFO power and external monitoring, and we found that CFO power magnifies the negative effect of female CFO on violations; the more the power, the more the negative effect of female CFO on violations. We also found that when the firm has effective external monitoring, there are fewer future infractions of information disclosure.
The Sequence Recall Task and Lexicality of Tone: Exploring Tone “Deafness”
Authors: Gussenhoven Carlos , Lu Yu-An , Lee-Kim Sang-Im , Liu Chunhui , Rahmani Hamed , Riad Tomas , Zora Hatice
Published: July 2022

Many perception and processing effects of the lexical status of tone have been found in behavioral, psycholinguistic, and neuroscientific research, often pitting varieties of tonal Chinese against non-tonal Germanic languages. While the linguistic and cognitive evidence for lexical tone is therefore beyond dispute, the word prosodic systems of many languages continue to escape the categorizations of typologists. One controversy concerns the existence of a typological class of “pitch accent languages,” another the underlying phonological nature of surface tone contrasts, which in some cases have been claimed to be metrical rather than tonal. We address the question whether the Sequence Recall Task (SRT), which has been shown to discriminate between languages with and without word stress, can distinguish languages with and without lexical tone. Using participants from non-tonal Indonesian, semi-tonal Swedish, and two varieties of tonal Mandarin, we ran SRTs with monosyllabic tonal contrasts to test the hypothesis that high performance in a tonal SRT indicates the lexical status of tone. An additional question concerned the extent to which accuracy scores depended on phonological and phonetic properties of a language’s tone system, like its complexity, the existence of an experimental contrast in a language’s phonology, and the phonetic salience of a contrast. The results suggest that a tonal SRT is not likely to discriminate between tonal and non-tonal languages within a typologically varied group, because of the effects of specific properties of their tone systems. Future research should therefore address the first hypothesis with participants from otherwise similar tonal and non-tonal varieties of the same language, where results from a tonal SRT may make a useful contribution to the typological debate on word prosody.
Searching on the Back: Attentional Selectivity in the Periphery of the Tactile Field
Authors: Gherri Elena , White Felicity , Ambron Elisabetta
Published: July 2022

Recent evidence has identified the N140cc lateralized component of event-related potentials as a reliable index of the deployment of attention to task-relevant items in touch. However, existing ERP studies have presented the tactile search array to participants' limbs, most often to the hands. Here, we investigated distractor interference effects when the tactile search array was presented to a portion of the body that is less lateralized and peripheral compared to the hands. Participants were asked to localize a tactile target presented among distractors in a circular arrangement to their back. The N140cc was elicited contralateral to the target when the singleton distractor was absent. Its amplitude was reduced when the singleton distractor was present and contralateral to the target, suggesting that attention was directed at least in part to the distractor when the singletons are on opposite sides. However, similar N140cc were observed when the singleton distractor was ipsilateral to the target compared to distractor absent trials. We suggest that when target and singleton distractor are ipsilateral, the exact localization of the target requires the attentional processing of all items on the same side of the array, similar to distractor absent trials. Together, these observations replicate the distractor interference effects previously observed for the hands, suggesting that analogous mechanisms guide attentional selectivity across different body parts.
A Biologically Inspired Neural Network Model to Gain Insight Into the Mechanisms of Post-Traumatic Stress Disorder and Eye Movement Desensitization and Reprocessing Therapy
Authors: Mattera Andrea , Cavallo Alessia , Granato Giovanni , Baldassarre Gianluca , Pagani Marco
Published: July 2022

Eye movement desensitization and reprocessing (EMDR) therapy is a well-established therapeutic method to treat post-traumatic stress disorder (PTSD). However, how EMDR exerts its therapeutic action has been studied in many types of research but still needs to be completely understood. This is in part due to limited knowledge of the neurobiological mechanisms underlying EMDR, and in part to our incomplete understanding of PTSD. In order to model PTSD, we used a biologically inspired computational model based on firing rate units, encompassing the cortex, hippocampus, and amygdala. Through the modulation of its parameters, we fitted real data from patients treated with EMDR or classical exposure therapy. This allowed us to gain insights into PTSD mechanisms and to investigate how EMDR achieves trauma remission.