bluuebunny/crossref_metadata_2025 huggingface dataset:
A probe for NIR-II imaging and multimodal analysis of early Alzheimer’s disease by targeting CTGF
Authors: Lu Cao , Meng Cong , Li Yuying , Yuan Jinling , Ren Xiaojun , Gao Liang , Su Dongdong , Cao Kai , Cui Mengchao , Yuan Qing , Gao Xueyun
Published: June 2024

To date, earlier diagnosis of Alzheimer’s disease (AD) is still challenging. Recent studies revealed the elevated expression of connective tissue growth factor (CTGF) in AD brain is an upstream regulator of amyloid-beta (Aβ) plaque, thus CTGF could be an earlier diagnostic biomarker of AD than Aβ plaque. Herein, we develop a peptide-coated gold nanocluster that specifically targets CTGF with high affinity (KD ~ 21.9 nM). The probe can well penetrate the blood-brain-barrier (BBB) of APP/PS1 transgenic mice at early-stage (earlier than 3-month-old) in vivo, allowing non-invasive NIR-II imaging of CTGF when there is no appearance of Aβ plaque deposition. Notably, this probe can also be applied to measuring CTGF on postmortem brain sections by multimodal analysis, including fluorescence imaging, peroxidase-like chromogenic imaging, and ICP-MS quantitation, which enables distinguishment between the brains of AD patients and healthy people. This probe possesses great potential for precise diagnosis of earlier AD before Aβ plaque formation.
Structural basis for urate recognition and apigenin inhibition of human GLUT9
Authors: Shen Zilin , Xu Li , Wu Tong , Wang Huan , Wang Qifan , Ge Xiaofei , Kong Fang , Huang Gaoxingyu , Pan Xiaojing
Published: June 2024

Urate, the physiological form of uric acid and a potent antioxidant in serum, plays a pivotal role in scavenging reactive oxygen species. Yet excessive accumulation of urate, known as hyperuricemia, is the primary risk factor for the development of gout. The high-capacity urate transporter GLUT9 represents a promising target for gout treatment. Here, we present cryo-electron microscopy structures of human GLUT9 in complex with urate or its inhibitor apigenin at overall resolutions of 3.5 Å and 3.3 Å, respectively. In both structures, GLUT9 exhibits an inward open conformation, wherein the substrate binding pocket faces the intracellular side. These structures unveil the molecular basis for GLUT9’s substrate preference of urate over glucose, and show that apigenin acts as a competitive inhibitor by occupying the substrate binding site. Our findings provide critical information for the development of specific inhibitors targeting GLUT9 as potential therapeutics for gout and hyperuricemia.
Structure of cryptophyte photosystem II–light-harvesting antennae supercomplex
Authors: Zhang Yu-Zhong , Li Kang , Qin Bing-Yue , Guo Jian-Ping , Zhang Quan-Bao , Zhao Dian-Li , Chen Xiu-Lan , Gao Jun , Liu Lu-Ning , Zhao Long-Sheng
Published: June 2024

Cryptophytes are ancestral photosynthetic organisms evolved from red algae through secondary endosymbiosis. They have developed alloxanthin-chlorophyll a/c2-binding proteins (ACPs) as light-harvesting complexes (LHCs). The distinctive properties of cryptophytes contribute to efficient oxygenic photosynthesis and underscore the evolutionary relationships of red-lineage plastids. Here we present the cryo-electron microscopy structure of the Photosystem II (PSII)–ACPII supercomplex from the cryptophyte Chroomonas placoidea. The structure includes a PSII dimer and twelve ACPII monomers forming four linear trimers. These trimers structurally resemble red algae LHCs and cryptophyte ACPI trimers that associate with Photosystem I (PSI), suggesting their close evolutionary links. We also determine a Chl a-binding subunit, Psb-γ, essential for stabilizing PSII–ACPII association. Furthermore, computational calculation provides insights into the excitation energy transfer pathways. Our study lays a solid structural foundation for understanding the light-energy capture and transfer in cryptophyte PSII–ACPII, evolutionary variations in PSII–LHCII, and the origin of red-lineage LHCIIs.
Three-dimensional chromatin reorganization regulates B cell development during ageing
Authors: Ma Fei , Cao Yaqiang , Du Hansen , Braikia Fatima Zohra , Zong Le , Ollikainen Noah , Bayer Marc , Qiu Xiang , Park Bongsoo , Roy Roshni , Nandi Satabdi , Sarantopoulou Dimitra , Ziman Andrew , Bianchi Aisha Haley , Beerman Isabel , Zhao Keji , Grosschedl Rudolf , Sen Ranjan
Published: June 2024

The contribution of three-dimensional genome organization to physiological ageing is not well known. Here we show that large-scale chromatin reorganization distinguishes young and old bone marrow progenitor (pro-) B cells. These changes result in increased interactions at the compartment level and reduced interactions within topologically associated domains (TADs). The gene encoding Ebf1, a key B cell regulator, switches from compartment A to B with age. Genetically reducing Ebf1 recapitulates some features of old pro-B cells. TADs that are most reduced with age contain genes important for B cell development, including the immunoglobulin heavy chain (Igh) locus. Weaker intra-TAD interactions atIghcorrelate with altered variable (V), diversity (D) and joining (J) gene recombination. Our observations implicate three-dimensional chromatin reorganization as a major driver of pro-B cell phenotypes that impair B lymphopoiesis with age.
GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior
Authors: Bhat Aaqib M. , Mohapatra Bhopal C. , Luan Haitao , Mushtaq Insha , Chakraborty Sukanya , Kumar Siddhartha , Wu Wangbin , Nolan Ben , Dutta Samikshan , Storck Matthew D. , Schott Micah , Meza Jane L. , Lele Subodh M. , Lin Ming-Fong , Cook Leah M. , Corey Eva , Morrissey Colm , Coulter Donald W. , Rowley M. Jordan , Natarajan Amarnath , Datta Kaustubh , Band Vimla , Band Hamid
Published: June 2024

While better management of loco-regional prostate cancer (PC) has greatly improved survival, advanced PC remains a major cause of cancer deaths. Identification of novel targetable pathways that contribute to tumor progression in PC could open new therapeutic options. The di-ganglioside GD2 is a target of FDA-approved antibody therapies in neuroblastoma, but the role of GD2 in PC is unexplored. Here, we show that GD2 is expressed in a small subpopulation of PC cells in a subset of patients and a higher proportion of metastatic tumors. Variable levels of cell surface GD2 expression were seen on many PC cell lines, and the expression was highly upregulated by experimental induction of lineage progression or enzalutamide resistance in CRPC cell models. GD2high cell fraction was enriched upon growth of PC cells as tumorspheres and GD2high fraction was enriched in tumorsphere-forming ability. CRISPR-Cas9 knockout (KO) of the rate-limiting GD2 biosynthetic enzyme GD3 Synthase (GD3S) in GD2high CRPC cell models markedly impaired the in vitro oncogenic traits and growth as bone-implanted xenograft tumors and reduced the cancer stem cell and epithelial-mesenchymal transition marker expression. Our results support the potential role of GD3S and its product GD2 in promoting PC tumorigenesis by maintaining cancer stem cells and suggest the potential for GD2 targeting in advanced PC.
A 10-week play-based after-school program to improve coordinative abilities and physical fitness capabilities among adolescents: a randomized trial
Authors: Kurnaz M. , Flôres F. , Altınkök M. , Esen H. T. , Silva A. F.
Published: June 2024

The average levels of physical activity in children are below the ideal, which may influence children's health and motor competence levels. Therefore, this study aimed to assess the impact of a 10-week play-based after-school program on 50 twelve-year-old students’ anthropometric characteristics (body height and body weight), coordinative abilities (flamingo balance test and T-test agility), and physical fitness (sit and reach, 20-m sprint test, SLJ, CMJ, and handgrip). After baseline assessments, the students were randomly divided into two groups: one participating in a play-based after-school program (experimental group) and the other attending regular PE classes (control group). No differences were noted between the sexes. Analysis revealed baseline differences between groups in body weight, agility T-test, and right-handgrip, with no significant sex differences. Following the intervention, the experimental group demonstrated improvements in the 20-m sprint test (F(1,46) = 11.03, p < 0.01), flamingo balance test (F(1,46) = 9.16, p = 0.004), SLJ (F(1,46) = 5.30, p = 0.03), agility T-test (F(1,46) = 28.30, p < 0.01), and right-handgrip (F(1,46) = 6.59, p < 0.01). In summary, the results suggested that a 10-week play-based after-school program enhances coordinative abilities and physical fitness in 12-year-old children. This underscores the potential advantages of integrating play-based physical activities into schools to promote holistic health and fitness in children.
Variation in microplastic characteristics among amphibian larvae: a comparative study across different species and the influence of human activity
Authors: Szkudlarek Michał , Najbar Bartłomiej , Jankowiak Łukasz
Published: June 2024

Microplastic pollution is a significant global environmental issue, and impacts span from individual organisms to the entire ecosystems. This study investigated the properties of microplastics in amphibian larvae, shedding light on their environmental interactions and potential ecological consequences. We examined microplastics extracted from amphibian larvae of 10 taxa, sampled from sites experiencing different levels of human impact. Our findings revealed a predominance of blue microplastics and fibres, each comprising 53% of the total microplastics in amphibian larvae. Microplastic fibres were also notably longer than other morphological types of microplastics. Furthermore, we observed variations in the surface area of microplastics among different amphibian families. An interesting observation from our research is the apparent positive relationship between the size of amphibian larvae and the length of granular and flake-shaped microplastics. Conversely, we observed a negative relationship between the length of these microplastics and human environmental impact. These insights significantly contribute to the understanding of microplastic pollution in freshwater environments, highlighting its complexities beyond marine ecosystems. Our research emphasises the intricate relationships between microplastics and freshwater organisms, underscoring the need for comprehensive strategies to mitigate microplastic pollution.
Understanding noble gas incorporation in mantle minerals: an atomistic study
Authors: Lora Alfredo , Patron Paola , Elena Alin M. , Allan Neil L. , Pinilla Carlos
Published: June 2024

Ab initio calculations in forsterite (Mg$$_2$$

2

SiO$$_4$$

4

) are used to gain insight into the formation of point defects and incorporation of noble gases. We calculate the enthalpies of incorporation both at pre-existing vacancies in symmetrically non-equivalent sites, and at interstitial positions. At high pressure, most structural changes affect the MgO$$_{6}$$

6

units and the enthalpies of point defects increase, with those involving Mg and Si vacancies increasing more than those involving O sites. At 15 GPa Si vacancies and Mg interstitials have become the predominant intrinsic defects. We use these calculated enthalpies to estimate the total uptake of noble gases into the bulk crystal as a function of temperature and pressure both in the presence and absence of other heterovalent trace elements. For He and Ne our calculated solubilities point to atoms occupying mainly interstitial sites in agreement with previous experimental work. In contrast, Ar most likely substitutes for Mg due to its larger size and the deformation it causes within the crystal. Incorporation energies, as well as atomic distances suggest that the incorporation mainly depend on the size mismatch between host and guest atoms. Polarization effects arising from the polarizability of the noble gas atom or the presence of charged defects are minimal and do not contribute significantly to the uptake. Finally, the discrepancies between our results and recent experiments suggest that there are other incorporation mechanisms such as adsorption at internal and external interfaces, voids and grain boundaries which must play a major role in noble gas storage and solubility.
PDL1 targeting by miR-138-5p amplifies anti-tumor immunity and Jurkat cells survival in non-small cell lung cancer
Authors: Rostami Fatemeh , Tavakol Hamedani Zahra , Sadoughi Azadeh , Mehrabadi Marzieh , kouhkan Fatemeh
Published: June 2024

Non-small cell lung cancer (NSCLC) has constituted over 80% of the lung cancer population with a poor prognosis. Over the past decade, immunotherapy has been constructed in the enlargement of immune checkpoint inhibitors as a promising approach for NSCLC treatment. Evading the immune system using the PD-1/PD-L1 axis is an intelligent way for cancers, and T cells cannot respond fully and confront cancer. Recently, the miR-138 was reported as a PD-L1 regulator in NSCLC. However, its inhibitory impact on T-cell exhaustion has not been characterized. The present study aims to impair PD-L1 (B7-H1) expression in Adenocarcinoma cell lines using miR-138-5p and determines how it prevents Jurak cell exhaustion. To gain the purpose, first, 18 highly significant dysregulated miRNAs containing hsa-miR-138 and CD274-mRNA network were detected in NSCLC based on bioinformatics analysis. Moreover, our study revealed a high level of miR-138-5p could make significant changes like PDL1 downregulation, proliferation, and mortality rate in A549/Calu6 cells. We also simulate cancer environmental conditions by culturing Jurak cells and NSCLC cell lines under the influence of stimulator cytokines to show how miR-138-5p survives Jurak cells by targeting PD-L1/PD-1pathway.
Arabic-language digital interventions for depression in German routine health care are acceptable, but intervention adoption remains a challenge
Authors: Reich Hanna , Hegerl Ulrich , Rosenthal Anja , Allenhof Caroline
Published: June 2024

Migrants face many barriers to mental health care, such as different cultural concepts of distress, unfamiliar pathways to care, and language. Digital mental health interventions are effective and scalable in multi-language versions. However, their implementation into routine care is in its infancy. Here, we report on the Arabic- and German-language implementation of two digital interventions in Germany: The iFightDepression® website, providing information about depression to the public, and the iFightDepression® tool, offering guided self-management for depression. Our main goal is to gain empirical knowledge about the success of their implementation and provide evidence-based recommendations for improvement. Data for the current analyses stem from convenience samples, utilizing anonymized user logs of the iFightDepression® website and 15.307 user accounts in the iFightDepression® tool. We found that the acceptability (time on page, usage behavior) of both digital interventions was comparable between the two user groups. The website pervasiveness of the target populations was nine times lower among Arab migrants in Germany than Germans (89 vs. 834 unique page views/ 100,000 inhabitants), but the increase in views was superior and sustained over three years. The adoption of the tool was lower among Arabic than German users (conversion rate from invitation to completed registration: 30.8% vs. 59.0%, p < 0.001) and appropriateness was challenged as Arabic users reported higher depression severities upon first registration (p = 0.027). Our results show that the uptake of digital interventions for migrants requires facilitation and further tailoring to the needs of the target group.
Metal concentrations and KIM-1 levels in school-aged children: a cross-sectional study
Authors: Mendoza‐Cano Oliver , Ríos‐Silva Mónica , Gonzalez-Curiel Irma , Camacho-delaCruz Arlette A. , Romo-García María Fernanda , Cuevas-Arellano Herguin Benjamin , Quintanilla‐Montoya Ana Luz , Martínez-Preciado Miguel A. , Rincón-Avalos Pedro , Hilerio-López Ángel Gabriel , Murillo‐Zamora Efrén
Published: June 2024

Environmental exposure to heavy metals and metalloids, originating from sources such as mining and manufacturing activities, has been linked to adverse renal effects. This cross-sectional study assessed children's exposure to these elements and its association with urinary kidney injury molecule-1 (KIM-1). We analyzed data from 99 school-aged children residing in nine localities within the state of Colima, Mexico, during the latter half of 2023. Levels of 23 metals/metalloids and urinary KIM-1 were measured using inductively coupled plasma mass spectrometry (ICP-MS) and enzyme-linked immunosorbent assay, respectively. Detectable levels of these contaminants were found in over 91% of participants, with varied exposure profiles observed across locations ($$p$$
p
= 0.019). After adjusting for confounding factors like gender, age, and locality, higher levels of six metals/metalloids (boron, cadmium, cesium, lithium, selenium, zinc) were significantly associated with increased KIM-1 levels. Tailored mitigation efforts are crucial to protect children from regional pollutant burdens. However, limitations exist, as our study did not capture all potential factors influencing heavy metal/metalloid and KIM-1 levels.
Molluscs generate preferred crystallographic orientation of biominerals by organic templates, the texture and microstructure of Caudofoveata (Aplacophora) shells
Authors: Yin X. , Castro-Claros J. D. , Griesshaber E. , Salas C. , Sancho Vaquer A. , Checa A. G. , Schmahl W. W.
Published: June 2024

Caudofoveata are molluscs that protect their vermiform body with a scleritome, a mosaic of unconnected blade/lanceolate-shaped aragonite sclerites. For the speciesFalcidens gutturosusandScutopus ventrolineatuswe studied the crystallographic constitution and crystal orientation texture of the sclerites and the scleritome with electron-backscatter-diffraction (EBSD), laser-confocal-microscopy (LCM) and field-emission electron microscopy (FE-SEM) imaging. Each sclerite is an aragonite single crystal that is completely enveloped by an organic sheath. Adjacent sclerites overlap laterally and vertically are, however, not connected to each other. Sclerites are thickened in their central portion, relative to their periphery. Thickening increases also from sclerite tip towards its base. Accordingly, cross-sections through a sclerite are straight at its tip, curved and bent towards the sclerite base. Irrespective of curved sclerite morphologies, the aragonite lattice within the sclerite is coherent. Sclerite aragonite is not twinned. For each sclerite the crystallographic c-axis is parallel to the morphological long axis of the sclerite, the a-axis is perpendicular to its width and the b-axis is within the width of the sclerite. The single-crystalinity of the sclerites and their mode of organization in the scleritome is outstanding. Sclerite and aragonite arrangement in the scleritome is not given by a specific crystal growth mode, it is inherent to the secreting cells. We discuss that morphological characteristics of the sclerites and crystallographic preferred orientation (texture) of sclerite aragonite is not the result of competitive growth selection. It is generated by the templating effect of the organic substance of the secreting cells and associated extracellular biopolymers.
Applying taper function models for black locust plantations in Greek post-mining areas
Authors: Wilms Florian , Berendt Ferréol , Bronisz Karol , Bashutska Ulyana , Fotelli Mariangela , Radoglou Kalliopi , Spyroglou Gavriil
Published: June 2024

A key process in forest management planning is the estimation of tree volume and, more specifically, merchantable volume. The ability to predict the cumulative stem volume relative to any upper stem diameter on standing trees or stands is essential for forest inventories and the management of forest resources. In the 1980s, the Hellenic Public Power Corporation (HPPC) started the rehabilitation of lignite post-mining areas in Greece by planting mainly black locust (Robinia pseudoacacia, L.). Today, these plantations occupy an area of approximately 2570 ha, but the stem volume has not yet been estimated. Therefore, we aimed to estimate the over- and under-bark stem volume using taper function models for 30 destructively sampled trees. Of the nineteen calibrated fixed-effects models, Kozak’s (2004) equation performed best for both the over-bark and under-bark datasets, followed by Lee’s (2003) and Muhairwe’s (1999) equations. Two fixed effect models were compared with fitted coefficients from Poland and the United States confirming that the local model fits were better suited, as the foreign model coefficients caused an increase in root mean square error (RMSE) for stem diameter predictions of 13% and 218%, respectively. The addition of random effects on a single-stem basis for two coefficients of Kozak’s (2004) equation improved the model fit significantly at 86% of the over-bark fixed effect RMSE and 69% for the under-bark model. Integrated taper functions were found to slightly outperform three volume equations for predictions of single stem volume over and under bark. Ultimately it was shown that these models can be used to precisely predict stem diameters and total stem volume for the population average as well as for specific trees of the black locust plantations in the study area.
Green synthesis of zinc oxide nanoparticles using Rhus coriaria extract and their anticancer activity against triple-negative breast cancer cells
Authors: Mongy Youssef , Shalaby Thanaa
Published: June 2024

The growing interest in using plant extracts for the biogenic synthesis of zinc oxide nanoparticles (ZnO NPs) stems from their facile, eco-friendly, and biologically safe approach instead of chemical routes. For the first time, ZnO NPs were successfully biosynthesized using Rhus coriaria fruit aqueous extract as a reducing and capping agent. Characterization revealed that the biosynthesized ZnO NPs possessed a maximum absorbance of approximately 359 nm and closely resembled the hexagonal ZnO wurtzite crystalline structure, with an average crystalline size of 16.69 nm. The transmission electron microscope (TEM) showed the presence of spherical and hexagonal morphologies, with an average grain size of 20.51 ± 3.90 nm. Moreover, the elemental composition of the synthesized ZnO NPs was assessed via energy-dispersive X-ray spectrometry (EDX), and the presence of phytocompounds on their surface was subsequently verified through FT-IR analysis. The ζ-potential of ZnO NPs was recorded at − 19.9 ± 0.1663 mV. Regarding anti-cancer properties, ZnO NPs were found to possess potent anti-tumor effects on MCF-7 and MDA-MB-231 breast cancer cells. Their efficacy was dose-dependent, with IC50 values ranging from 35.04–44.86 μg/mL for MCF-7 and 55.54–63.71 µg/mL for MDA-MB-231 cells. Mechanistic studies in MDA-MB-231 cells revealed apoptosis induction, validated by DAPI staining, confocal microscopy, and Annexin V/PI staining, showing apoptosis by 12.59% and 81.57% at ½ IC50 and IC50 values, respectively. Additionally, ZnO NPs were observed to provoke S-phase arrest and inhibit colony-forming and metastatic potential by modulating apoptosis and metastasis-related genes. This study unravels new insights into how ZnO NPs provoke cancer cell death and inhibit metastasis, revealing new prospects in cancer nanotechnology.
Improving prediction accuracy of laser-induced shock wave velocity prediction using neural networks
Authors: Yang Haoyu
Published: June 2024

The velocity of laser-induced shock waves affects the efficiency and efficacy of laser-based processes. The ability to accurately estimate shock wave velocity is critical for optimizing experimental combinations, creating laser-based systems, and assuring desired results. Traditional approaches to predict shock wave velocity involve empirical equations and analytical models based on simplified assumptions. However, these methods often lack accuracy and fail to capture the complex dynamics of laser-matter interactions. To overcome these limitations, we used a combination of an artificial neural network and a genetic algorithm to predict shock wave velocity. In this method, the neural network structure is dynamically designed. The optimization method does this by modifying the neural network's weights and figuring out the network's structure on our behalf. Based on the findings, our suggested technique worked very well; it surpassed other comparison methods by achieving the lowest average errors in terms of RMSE and MAE, which are 4.38 and 3.74, respectively. Moreover, the analysis has shown that our proposed method has a high level of reliability in predicting impulsive wave velocity using a neural network.
Small RNA signatures of acute ischemic stroke in L1CAM positive extracellular vesicles
Authors: Manwani Bharti , Brathaban Nivetha , Baqai Abiya , Munshi Yashee , Ahnstedt Hilda W. , Zhang Mengqi , Arkelius Kajsa , Llera Ted , Amorim Edilberto , Elahi Fanny M. , Singhal Neel S.
Published: June 2024

L1CAM-positive extracellular vesicles (L1EV) are an emerging biomarker that may better reflect ongoing neuronal damage than other blood-based biomarkers. The physiological roles and regulation of L1EVs and their small RNA cargoes following stroke is unknown. We sought to characterize L1EV small RNAs following stroke and assess L1EV RNA signatures for diagnosing stroke using weighted gene co-expression network analysis and random forest (RF) machine learning algorithms. Interestingly, small RNA sequencing of plasma L1EVs from patients with stroke and control patients (n = 28) identified micro(mi)RNAs known to be enriched in the brain. Weighted gene co-expression network analysis (WGCNA) revealed small RNA transcript modules correlated to diagnosis, initial NIH stroke scale, and age. L1EV RNA signatures associated with the diagnosis of AIS were derived from WGCNA and RF classification. These small RNA signatures demonstrated a high degree of accuracy in the diagnosis of AIS with an area under the curve (AUC) of the signatures ranging from 0.833 to 0.932. Further work is necessary to understand the role of small RNA L1EV cargoes in the response to brain injury, however, this study supports the utility of L1EV small RNA signatures as a biomarker of stroke.
Physical structure of the environment contributes to the development of diversity of microalgal assemblages
Authors: Lukács Áron , Szabó Sándor , T-Krasznai Enikő , Görgényi Judit , Tóth István , B-Béres Viktória , Lerf Verona , Nemes-Kókai Zsuzsanna , Borics Gábor
Published: June 2024

Aquatic macrophytes form a three dimensional complex structure in the littoral zones of lakes, with many physical, chemical and biological gradients and interactions. This special habitat harbours a unique microalgal assemblage called metaphyton, that differs both from the phytoplankton of the pelagial and from the benthic assemblages whose elements are tightly attached to the substrates. Since metaphytic assemblages significantly contribute to the diversity of lakes’ phytoplankton, it is crucial to understand and disentangle those mechanisms that ensure their development. Therefore, we focused on the question of how a single solid physical structure contribute to maintaining metaphytic assemblages. Using a laboratory experiment we studied the floristic and functional differences of microalgal assemblages in microcosms that simulated the conditions that an open water, a complex natural macrophyte stand (Utricularia vulgaris L.), or an artificial substrate (cotton wool) provide for them. We inoculated the systems with a species rich (> 326 species) microalgal assemblage collected from a eutrophic oxbow lake, and studied the diversity, trait and functional group composition of the assemblages in a 24 day long experimental period. We found that both natural and artificial substrates ensured higher species richness than the open water environment. Functional richness in the open water environment was lower than in the aquaria containing natural macrophyte stand but higher than in which cotton wool was placed. This means that the artificial physical structure enhanced functional redundancy of the resident functional groups. Elongation measures of microalgal assemblages showed the highest variation in the microcosms that simulated the open water environment. Our results suggest that assembly of metaphytic algal communities is not a random process, instead a deterministic one driven by the niche characteristics of the complex three dimensional structure created by the stands of aquatic macrophytes.
Circulating immunoglobulins and transient lymphocytopenia in a sub-study of CAPRISA 012B, testing HIV monoclonal antibodies in a phase 1 trial
Authors: Sobia Parveen , Mahomed Sharana , Sivro Aida , Paul Stephane , Osman Farzana , Harkoo Ishana , Garrett Nigel , Karim Quarraisha Abdool , Karim Salim S. Abdool , Archary Derseree
Published: June 2024

Acute, transient lymphocytopenia, not clinically significant was observed in the CAPRISA 012B phase 1 clinical trial following administration of broadly neutralizing antibodies (bnAb)-CAP256V2LS alone or with VRC07-523LS. Lymphocytopenia was assigned upon a > 50% decline in absolute lymphocyte counts following bnAb administration. We posited that systemic immunoglobulins (Igs), and cytokine profiles of eight women who developed lymphocytopenia were different to the 12 women without lymphocytopenia. Plasma Ig subclasses (IgG)/isotypes (IgM/IgA), and 27 cytokines were measured at enrolment (prior to bnAbs) and at days 1, 7, 28, 56 post-bnAb administration. IgG subclasses, IgM and total lymphocyte counts were significantly lower prior to bnAbs in women with gradable lymphocytopenia than those without. Gradable lymphocytopenia compared to non-lymphocytopenia women had significantly higher MIP-1β from enrolment up to day 56. TNF-α was significantly lower in gradable lymphocytopenia compared to non-lymphocytopenia women for enrolment, days 7, 28 and 56 except for day 1. Within the gradable and within the non-lymphocytopenia women, from enrolment to day 1, significantly elevated IL-6, IL-8, IP-10, MCP-1, G-CSF and IL-1RA were found. Additionally, within the gradable lymphocytopenia women, 9 additional cytokines (TNF-α, MIP-1α, MIP-1β, RANTES, Basic FGF, eotaxin, IFN-γ, IL-17A and IL-4) were significantly elevated at day 1 post-bnAbs compared to enrolment. This sub study presents preliminary findings to support the monitoring of baseline immunological markers including lymphocyte counts for assessing the development of transient lymphocytopenia. In high-risk settings conducting clinical trials testing bnAbs for HIV prevention, understanding factors that could amplify rates of lymphocytopenia, even if transient, remain undefined.
Integrated metaheuristic algorithms with extreme learning machine models for river streamflow prediction
Authors: Van Thieu Nguyen , Nguyen Ngoc Hung , Sherif Mohsen , El-Shafie Ahmed , Ahmed Ali Najah
Published: June 2024

Accurate river streamflow prediction is pivotal for effective resource planning and flood risk management. Traditional river streamflow forecasting models encounter challenges such as nonlinearity, stochastic behavior, and convergence reliability. To overcome these, we introduce novel hybrid models that combine extreme learning machines (ELM) with cutting-edge mathematical inspired metaheuristic optimization algorithms, including Pareto-like sequential sampling (PSS), weighted mean of vectors (INFO), and the Runge–Kutta optimizer (RUN). Our comparative assessment includes 20 hybrid models across eight metaheuristic categories, using streamflow data from the Aswan High Dam on the Nile River. Our findings highlight the superior performance of mathematically based models, which demonstrate enhanced predictive accuracy, robust convergence, and sustained stability. Specifically, the PSS-ELM model achieves superior performance with a root mean square error of 2.0667, a Pearson’s correlation index (R) of 0.9374, and a Nash–Sutcliffe efficiency (NSE) of 0.8642. Additionally, INFO-ELM and RUN-ELM models exhibit robust convergence with mean absolute percentage errors of 15.21% and 15.28% respectively, a mean absolute errors of 1.2145 and 1.2105, and high Kling-Gupta efficiencies values of 0.9113 and 0.9124, respectively. These findings suggest that the adoption of our proposed models significantly enhances water management strategies and reduces any risks.
Expressive suppression mediates the relationship between sleep quality and generalized anxiety symptomology
Authors: Bendall Robert C. A. , Elton Sophie N. , Hughes Alun T. L.
Published: June 2024

Anxiety disorders are the most prevalent worldwide mental health disorder, resulting in high societal costs. Emotion regulation and sleep quality are associated with the development of psychopathologies including anxiety. However, it is unknown whether habitual emotion regulation strategy use can mediate the influence of sleep quality on anxiety symptomology. An opportunity sample in a healthy population completed the Pittsburgh Sleep Quality Index to provide a measure of sleep quality, the Emotion Regulation Questionnaire to assess habitual use of emotion regulation strategies, and the Generalized Anxiety Disorder Scale to record anxiety symptomology. Data were analysed using correlation and regression-based mediation analyses. Improved sleep quality was predictive of reduced habitual use of expressive suppression and reduced anxiety symptomology. Additionally, increased use of expressive suppression was predictive of greater anxiety symptomology. Cognitive reappraisal was not associated with sleep quality or anxiety severity. Further, novel findings using mediation analyses show that expressive suppression partially mediated the relationship between sleep quality and anxiety. Whilst longitudinal and experimental research are needed to establish causality, these findings suggest that simultaneously targeting improvements in sleep quality and the use of specific emotion regulation strategies, including expressive suppression, may improve the efficacy of interventions focussed on reducing anxiety-related symptomology.
In-depth analysis of ChatGPT’s performance based on specific signaling words and phrases in the question stem of 2377 USMLE step 1 style questions
Authors: Knoedler Leonard , Knoedler Samuel , Hoch Cosima C. , Prantl Lukas , Frank Konstantin , Soiderer Laura , Cotofana Sebastian , Dorafshar Amir H. , Schenck Thilo , Vollbach Felix , Sofo Giuseppe , Alfertshofer Michael
Published: June 2024

ChatGPT has garnered attention as a multifaceted AI chatbot with potential applications in medicine. Despite intriguing preliminary findings in areas such as clinical management and patient education, there remains a substantial knowledge gap in comprehensively understanding the chances and limitations of ChatGPT’s capabilities, especially in medical test-taking and education. A total of n = 2,729 USMLE Step 1 practice questions were extracted from the Amboss question bank. After excluding 352 image-based questions, a total of 2,377 text-based questions were further categorized and entered manually into ChatGPT, and its responses were recorded. ChatGPT’s overall performance was analyzed based on question difficulty, category, and content with regards to specific signal words and phrases. ChatGPT achieved an overall accuracy rate of 55.8% in a total number of n = 2,377 USMLE Step 1 preparation questions obtained from the Amboss online question bank. It demonstrated a significant inverse correlation between question difficulty and performance with rs = -0.306; p < 0.001, maintaining comparable accuracy to the human user peer group across different levels of question difficulty. Notably, ChatGPT outperformed in serology-related questions (61.1% vs. 53.8%; p = 0.005) but struggled with ECG-related content (42.9% vs. 55.6%; p = 0.021). ChatGPT achieved statistically significant worse performances in pathophysiology-related question stems. (Signal phrase = “what is the most likely/probable cause”). ChatGPT performed consistent across various question categories and difficulty levels. These findings emphasize the need for further investigations to explore the potential and limitations of ChatGPT in medical examination and education.
Money for operator: the impact of linked agricultural subsidy on incomes
Authors: Yi Haoping , Guo Dengwang , Wang Haomin , Yi Guohui , Min Longzhen
Published: June 2024

The reform of China’s “three subsidies” has shifted the method of subsidization from payment based on the contracted area to payment based on the actual operational area. Within this context, studying the income-generating impact of the “three subsidies” holds significant practical relevance. Using data from the 2018 China Labor-force Dynamic Survey, this paper employs basic estimation, mediating effect, and moderating effect models to analyze the heterogeneity of agricultural subsidies’ impact on rural household income, the mediating effect of agricultural mechanization, and the moderating effect of operation scale. Our findings indicate that agricultural subsidies, known as the “three subsidies”, have increased total rural household income and agricultural income while decreasing wage income. However, they have shown no significant impact on business income. Notably, agricultural subsidies have significantly elevated the income of food-producing households, with agricultural mechanization partially mediating this effect. Operation scale positively moderates the impact of agricultural subsidies on rural household income and agricultural mechanization. Heterogeneity analysis indicates that agricultural subsidies have a more significant impact on rural household income among agricultural producers in the eastern region.
MRGPRX2-mediated mast cell activation by substance P from overloaded human tenocytes induces inflammatory and degenerative responses in tendons
Authors: Mousavizadeh Rouhollah , Waugh Charlie M. , McCormack Robert G. , Cairns Brian E. , Scott Alex
Published: June 2024

Mast cells are immune cells minimally present in normal tendon tissue. The increased abundance of mast cells in tendinopathy biopsies and at the sites of tendon injury suggests an unexplored role of this cell population in overuse tendon injuries. Mast cells are particularly present in tendon biopsies from patients with more chronic symptom duration and a history of intensive mechanical loading. This study, therefore, examined the cross talk between mast cells and human tendon cells in either static or mechanically active conditions in order to explore the potential mechanistic roles of mast cells in overuse tendon injuries. A coculture of isolated human tenocytes and mast cells (HMC-1) combined with Flexcell Tension System for cyclic stretching of tenocytes was used. Additionally, human tenocytes were exposed to agonists and antagonists of substance P (SP) receptors. Mast cell degranulation was assessed by measuring β-hexosaminidase activity. Transwell and cell adhesion assays were used to evaluate mast cell migration and binding to tendon extracellular matrix components (collagen and fibronectin), respectively. Gene expressions were analyzed using real time qRT-PCR. Our results indicate that mechanical stimulation of human tenocytes leads to release of SP which, in turn, activates mast cells through the Mas-related G-protein-coupled receptor X2 (MRGPRX2). The degranulation and migration of mast cells in response to MRGPRX2 activation subsequently cause human tenocytes to increase their expression of inflammatory factors, matrix proteins and matrix metalloproteinase enzymes. These observations may be important in understanding the mechanisms by which tendons become tendinopathic in response to repetitive mechanical stimulation.
Fabrication of binary metal–organic frameworks of Ni–Mn@ZIFs(Cox·Zn1−xO) decorated on CF/CuO nanowire for high-performance electrochemical pseudocapacitors
Authors: Momeni Abkharaki Ali , Ensafi Ali A.
Published: June 2024

Herein, metal–organic frameworks (MOFs) derived nanoflower-like based binary transition metal (Ni–Mn) are successfully fabricated by a simple synthesis method. The fabricated nanoflower-like structure displays a unique nanoflower-like architecture and internal porous channels constructed by MOF coated on CuO/CF/ZIFs (Cox·Zn1−xO) substrate, which is beneficial for the penetration of electrolyte and electron/ion transportation. The as-prepared CF/CuO/ZIFs (Cox·Zn1−xO)@BMOF(Ni–Mn) electrode materials present significant synergy among transition metal ions, contributing to enhanced electrochemical performances. The as-prepared CF/CuO/ZIFs (Cox·Zn1−xO)@BMOF(Ni–Mn) hybrid nanoflower-like display a high specific capacity of 1249.99 C g−1 at 1 A g−1 and the specific capacitance retention is about 91.74% after 5000 cycles. In addition, the as-assembled CF/CuO/ZIFs (Cox·Zn1−xO)@BMOF(Ni–Mn)//AC asymmetric supercapacitor (ASC) device exhibited a maximum energy density of 21.77 Wh·kg−1 at a power density of 799 W kg−1, and the capacity retention rate after 5000 charge and discharge cycles was 88.52%.
Optimal immobilization position for conservative treatment of proximal humerus fractures by fracture type: a biomechanical cadaveric study
Authors: Jin Seokhwan , Lim Joon-Ryul , Yoon Tae-Hwan , Choi Yun-Rak , Chun Yong-Min
Published: June 2024

In conservative treatment for proximal humerus fractures (PHFs), the immobilization position of the affected arm should not be determined uniformly. The aim of this study is to investigate the optimal immobilization position for conservative treatment of different types of PHFs. We hypothesized that the optimal position minimizing the deforming force in PHFs depends on the fracture components involved. PHF models involving either the surgical neck (SN) or greater tuberosity (GT) were created using 12 fresh-frozen cadaveric shoulders. In the SN model, the deforming forces on the pectoralis major muscle were measured in full adduction by increasing external rotation. In the GT model, the deforming force of the supraspinatus muscle was measured in neutral rotation by decreasing abduction, and the deforming force of the infraspinatus muscle was measured in full adduction by increasing internal rotation, respectively. In the SN model, the deforming force of the pectoralis major muscle increased significantly with external rotation from full internal rotation to neutral rotation (P = 0.006), indicating that the arm should be placed in full internal rotation. In the GT model, the deforming force of the supraspinatus muscle increased significantly with adduction from 45° of abduction to full adduction (P = 0.006); the deforming force of the infraspinatus muscle increased significantly with internal rotation from neutral rotation to full internal rotation (P = 0.006). These findings should be considered when placing the arm in abduction and neutral rotation so as to minimize the deforming force by either the supra or infraspinatus muscle. In conservative treatment for PHFs, the affected arm should be placed in a position that minimizes the deforming force on the fracture components involved.
Understanding the relationship between pore size, surface charge density, and Cu2+ adsorption in mesoporous silica
Authors: Niu Yanhui , Yu Wenbin , Yang Shuguang , Wan Quan
Published: June 2024

This research delved into the influence of mesoporous silica’s surface charge density on the adsorption of Cu2+. The synthesis of mesoporous silica employed the hydrothermal method, with pore size controlled by varying the length of trimethylammonium bromide (CnTAB, n = 12, 14, 16) chains. Gas adsorption techniques and transmission electron microscopy characterized the mesoporous silica structure. Surface charge densities of the mesoporous silica were determined through potentiometric titration, while surface hydroxyl densities were assessed using the thermogravimetric method. Subsequently, batch adsorption experiments were conducted to study the adsorption of Cu2+ in mesoporous silica, and the process was comprehensively analyzed using Atomic absorption spectrometry (AAS), Fourier transform infrared (FTIR), and L3 edge X-ray absorption near edge structure (XANES). The research findings suggest a positive correlation between the pore size of mesoporous silica, its surface charge density, and the adsorption capacity for Cu2+. More specifically, as the pore size increases within the 3–4.1 nm range, the surface charge density and the adsorption capacity for Cu2+ also increase. Our findings provide valuable insights into the relationship between the physicochemical properties of mesoporous silica and the adsorption behavior of Cu2+, offering potential applications in areas such as environmental remediation and catalysis.
Connectivity modelling identifies sources and sinks of coral recruitment within reef clusters
Authors: Ani Chinenye J. , Haller-Bull Vanessa , Gilmour James P. , Robson Barbara J.
Published: June 2024

Connectivity aids the recovery of populations following disturbances, such as coral bleaching and tropical cyclones. Coral larval connectivity is a function of physical connectivity and larval behaviour. In this study, we used OceanParcels, a particle tracking simulator, with 2D and 3D velocity outputs from a high resolution hydrodynamic-biogeochemical marine model (RECOM) to simulate the dispersal and settlement of larvae from broadcast spawning Acropora corals in the Moore Reef cluster, northern Great Barrier Reef, following the annual spawning events in 2015, 2016 and 2017. 3D velocity simulations showed 19.40–68.80% more links and sinks than those of 2D simulations. Although the patterns of connectivity among sites vary over days and years, coral larvae consistently dispersed from east to west in the cluster domain, with some sites consistently acting as sources or sinks for local larval recruitment. Results can inform coral reef intervention plans for climate change, such as the design of marine protected areas and the deployment of proposed interventions within reef clusters. For example, the wider benefits of interventions (e.g., deployment of heat adapted corals) may be optimised when deployed at locations that are a source of larvae to others within comparable habitats across the reef cluster.
Using QUASR-PCR as a field-based genotyping assay for a tick acaricide resistance marker
Authors: Meiring Christina , Labuschagne Michel
Published: June 2024

A novel, turnkey, field-based workflow was developed and validated using Rhipicephalus microplus DNA as a template to detect the presence of the voltage-gated sodium channel kdr mutation. The field-based compatible workflow comprises manual sample homogenization for DNA extraction, PCR amplification of the targets in a closed tube, and end-point detection of the PCR products. An R. microplus species-specific assay was also included to confirm species identity and ensure the validity of the kdr mutation assay. The assays were sensitive and specific to the targets, and the workflow resulted in a turnaround time of approximately 1 h at a low cost. The novel combination of PCR with closed-tube and end-point fluorescent detection allows for easy conversion of existing conventional lab-based PCR assays into field-based detection assays. The incorporation of custom-designed 3D-printed components in the workflow provides easy adaptability and modification of the components for diverse nucleic acid detection workflows.