bluuebunny/crossref_metadata_2025 huggingface dataset:
TEMPORAL VEGETATION DYNAMICS IN PEAT SWAMP AREA USING MODIS TIME-SERIES IMAGERY: A MONITORING APPROACH OF HIGH-SENSITIVE ECOSYSTEM IN REGIONAL SCALE
Authors: Setiawan Yudi , Pawitan Hidayat , Prasetyo Lilik Budi , Parlindungan May , Permatasari Prita Ayu
Published: October 2016

Peat swamp area is an essential ecosystem due to high vulnerability of functions and services. As the change of forest cover in peat swamp area has increased considerably, many studies on peat swamp have focused on forest conversion or forest degradation. Meanwhile, in the context of changes in the forestlands are the sum of several processes such as deforestation, reforestation/afforestation, regeneration of previously deforested areas, and the changing spatial location of the forest boundary. Remote sensing technology seems to be a powerful tool to provide information required following that concerns. A comparison imagery taken at the different dates over the same locations for assessing those changes tends to be limited by the vegetation phenology and land-management practices. Consequently, the simultaneous analysis seems to be a way to deal with the issues above, as a means for better understanding of the dynamics changes in peat swamp area. In this study, we examined the feasibility of using MODIS images during the last 14 years for detecting and monitoring the changes in peat swamp area. We identified several significant patterns that have been assigned as the specific peat swamp ecosystem. The results indicate that a different type of ecosystem and its response to the environmental changes can be portrayed well by the significant patterns. In understanding the complex situations of each pattern, several vegetation dynamics patterns were characterized by physical land characteristics, such as peat depth, land use, concessions and others. Characterizing the pathways of dynamics change in peat swamp area will allow further identification for the range of proximate and underlying factors of the forest cover change that can help to develop useful policy interventions in peatland management.
How I Do It: Temporary RVAD Placed at the Time of Implantation of a HeartMate II LVAD
Authors: Chancellor William Zachary , Tribble Curt
Published: October 2016

Left ventricular assist devices (LVAD) are increasingly used to support patients as they await heart transplantation and as destination therapy for patients with end-stage cardiac failure. While the methods of LVAD implantation have become fairly standardized, early postoperative management of patients receiving these devices remains challenging. One issue that has plagued surgeons, cardiologists, and intensivists caring for patients after LVAD implantation is right heart dysfunction. While many scoring systems have been developed to try to anticipate RV failure, the accuracy of these predictive tools remains low. We present a novel approach of implantation of a temporary right ventricular assist devices (RVAD) during LVAD implantation with subsequent weaning in the immediate postoperative period, utilizing a strategy that does not require a return to the operating room for removal of the RVAD cannulas.
Standardization of Inpatient Handoff Communication
Authors: Jewell Jennifer A. ,   COMMITTEE ON HOSPITAL CARE, Percelay Jack M. , Hill Vanessa L. , Preuschoff Claudia K. , Rauch Daniel A. , Salerno Richard A.
Published: October 2016

Handoff communication is identified as an integral part of hospital care. Throughout medical communities, inadequate handoff communication is being highlighted as a significant risk to patients. The complexity of hospitals and the number of providers involved in the care of hospitalized patients place inpatients at high risk of communication lapses. This miscommunication and the potential resulting harm make effective handoffs more critical than ever. Although hospitalized patients are being exposed to many handoffs each day, this report is limited to describing the best handoff practices between providers at the time of shift change.
ЗМІНИ ВЛАСТИВОСТЕЙ ЕРИТРОЦИТІВ У СОБАК
Authors: Anforova M.V. , Golovakha V.I. , Piddubnyak O.V. , Tyshkivskyy M.J.
Published: October 2016

One of the most important indicators is the assessment of erythroсytopoezis is acid resistance of erythrocytes and their population composition. Changes in these parameters are the most studied in humans, cattle and horses. In small animals, including dogs, the issue is not highlighted enough. Therefore, the study of changes of erytroсytopoezis by using these tests is important. The study erythroсytopoezis parameters was performed in dogs of all ages (1.5–year, 3 – 6 years and 7 and older years of age). It was found that in clinically healthy dogs generall erythroсytopoezis indicators (total number of red blood cells, hemoglobin content, hematocrit value, the index «red» blood – МСН and МСV), regardless of age, did not significantly change. Yet in dogs older than 7 years there were found some changes on the the part of erythroсytopoezis. This was pointed by the changes of the population composition of red blood cells and their acid resistance. In particular, the animals showed an increased number of «old» populations of red blood cells and some changes of their hemolytic resistance. Particularly, the height of the erytrohram peak was 15% higher than in younger animals. The very peak was sharp, indicating the rapid destruction of the «young» red cells and degeneration of their lipid membranes.
ОБСІМЕНІННЯ САЛЬМОНЕЛАМИ ЯЛОВИЧИНИ І СВИНИНИ, ЯКІ НАДХОДЯТЬ В РЕАЛІЗАЦІЮ З ПРИСАДИБНИХ І ФЕРМЕРСЬКИХ ГОСПОДАРСТВ
Authors: Brodovsky V. , Kovbasenko V.
Published: October 2016

Today in Ukraine, nearly all the meat that comes for sale on the agro–industrial markets, produced in farms and private households, where the conditions to receive it, unfortunately, do not guarantee its sanitary quality. Meat rarely get from sick animals are not always fulfilled the requirements for slaughtering and primary processing of carcasses, storage and transportation. Such violations may result in contamination of the slaughter of microorganisms, including salmonella, in turn, contribute to the occurrence of damage processes meat and risks of occurrence of food poisoning, and therefore is a threat to consumers.According to international requirements and the state «the minimum list of research ...» microflora content in meat rationed and microbiological research of meat and meat products are required. But in a state laboratories of veterinary–sanitary expertise in markets, livestock microbiologically investigate.Given the above, we examined the degree of contamination with salmonella carcasses of cattle and pigs that come for sale on the markets of agro farms and private households, salmonella. The study was carried out of the market. Mykolaiv. Research subject carcasses that passed veterinary and sanitary examination in terms of state laboratory of veterinary–sanitary examination of the market, the results of which were accepted for implementation.The results of our studies on pig carcasses 11.1% and 9.1% of carcasses of cattle that came to the market and were made to implement, isolated salmonella Worms for serological and biochemical properties were attributed to serovariantiv S. typhimurium (25.0% ), S. enteritidis (18.7%), S. cholera suis (37.5%) and S. raratyphi (18.7%).Further studies to determine the pathogenic properties of isolated cultures found that 93.7% of them are pathogenic properties inherent in varying degrees, and 18.7% of selected crops (serovarianty S. cholera suis and S. typhimurium) found a high degree of pathogenicity.Investigation of the thermal stability of isolated cultures set high temperature resistant crops serovariantiv S. cholera suis and S. typhimurium – death at a temperature of 90 °C drew after 10 minutes.Our results indicate that under the current system status monitoring safety of meat, there is no preventive aspect, required by international standards, and meat which is sold in the markets can be a source of food poisoning and salmonellosis etiology constitute a danger for the consumer.
КЛІНІКО–БІОХІМІЧНИЙ СТАТУС ДІЙНИХ КОРІВ ПАФ «НЕФЕДІВСЬКЕ» КАМ’ЯНЕЦЬ–ПОДІЛЬСЬКОГО РАЙОНУ ХМЕЛЬНИЦЬКОЇ ОБЛАСТІ
Authors: Koltun О.M. , Rusyn V.I.
Published: October 2016

These results of clinical and biochemical status of the herd of dairy cows PAF «Nefedivske» Kamenets–Podolsk district of Khmelnitsky region. The aim was to determine the clinical and biochemical status of the herd of dairy cows and its results to develop measures correcting the violations. Work performed at the PAF «Nefedivske» Kamenets–Podolsk district of Khmelnitsky region. The object of the research were lactating cows, black and white breeds, ages 4 – 6 years and daily milk yield 16 – 18 liters of milk.Survey livestock lactating cows clinically infected animals, which noted the following symptoms: drowsiness animals, reducing distortion and appetite, dullness and disheveled hair cover; delay molting; decrease skin resilience, dry it and thickening; anemic visible mucous membranes; reducing the number of periods and bovine milk production. Serum lactating cows found high content of inorganic phosphorus and iron, and low content of copper and zinc, which constitutes a violation of mineral metabolism. Patient’s hemocytopoesis animals found violations that characterized olihochromemia, decreased hematocrit values and average concentrations of hemoglobin, indicating the development of hypochromic anemia. According to the results of biochemical blood tests in lactating cows installed hipoproteinemia, hipoalbuminemia, and high activity of transaminases and alkaline phosphatase, indicating a violation of metabolic processes in the body sick animals. These results indicate the need for the development and management of correcting the violations of metabolism in dairy cows.
ЕПІЗООТОЛОГІЧНИЙ МОНІТОРИНГ СКАЗУ ТВАРИН У ЛЬВІВСЬКІЙ ОБЛАСТІ ЗА 2014–2016 РОКИ, АНАЛІЗ ПРОВЕДЕНИХ АНТИРАБІЧНИХ ЗАХОДІВ
Authors: Levkivsky D.M. , Levkivska N.D. , Storchak Yu. , Gutyj B.V.
Published: October 2016

It was found that the main source of rabies in our time are the foxes, the share of which exceeds 50% of the diseased animals. Probability bitten animal diseases depends on the number of supported bites, their location, extent of tissue damage, and amount of virus virulence.It notes some cyclicality in the emergence of rabies outbreaks. Periods of relative attenuation of infection are replaced with new lifts every 3 – 4 years. It was found that durig the period from 2014 to 2016 it was registered 57 unfavorable items rabies animals in the Lviv region. Among them: the fox – 36; dogs – 11; cats – 9; ferrets – 1. In 2014 eight disadvantaged areas with rabies foxes has been recorded, in 2015 – 8, 2016 – six disadvantaged areas. By the number of animal deaths the first place belongs to the foxes, the second dog place on the third and fourth cat – ferret. The weak link in the system of measures to combat rabies infection is the lack of work on the elimination of stray animals (dogs and cats) and a decrease in the population of foxes, as well as the failure to implement fully the annual vaccination of dogs and lack of oral immunization of wildlife species. Today a vaccine based on a recombinant strain «V–RG» for the oral immunization of wild carnivores registered in Ukraine, which is produced by LLC «Ukrvetprompostach», Brovary, Kiev region, Ukraine.
ГІПОХОЛЕСТЕРОЛЕМІЧНА ДІЯ N–АЛКІЛАМІДОВАНОГО АЛЬГІНАТУ
Authors: Marounek M. , Volek Z. , Taubner T. , Duškova D. , Kalachniuk L. , Khomych V.
Published: October 2016

It is known that in the process of digestion and absorption of nutrients, one of the key roles belongs to polysaccharides (among which there are alginic acid), which have the ability to increase viscosity and form a gel in the intestine. Previously, we studied the physiological effects of pectin, cellulose and their amidated derivatives that have demonstrated growth of hypocholesterolemic activity in the rats. From here, it was decided to investigate the hypocholesterolemic effect of N–alkylamidated alginate on laboratory animals. That’s why, female rats were fed according to three diets, namely the control (1) and two experimental (2 and 3), which contained cholesterol (10 g/kg) and different amounts N–alkylamidated alginate (0 and 40 g / kg, respectively), and all animals fed also palm oil. N–alkylamidated alginate significantly lowered the level of cholesterol (total and LDL) in serum and liver and of hepatic total lipids in the rats, which were on cholesterol–containing diet. In the faeces of animals that consumed N–alkylamidated alginate, it was observed increased concentrations of fat from 61 to 91 mg / g, while the concentration of cholesterol, bile acids and total sterols was not significantly changed.Hence, decrease cholesterolemia in rats (that consumed N–alkylamidated alginate) is probably based more on the removal of fat from the intestine, rather than on the interruption of enterohepatic circulation of cholesterol.
ВДОСКОНАЛЕННЯ СИСТЕМИ КОНТРОЛЮ ТА ОБІГУ ГМО В УКРАЇНІ
Authors: Nazar B.I.
Published: October 2016

The article briefly presents the main approaches to the state registration and order of the state veterinary and sanitary expertise GMO sources of feed, feed additives and veterinary medicinal products are listed feasibility studies to be carried out at the registration of GMO sources listed safety parameters on which the assessment of GMO sources. There need to conduct clinical studies to determine the impact of GMO sources or feed sources, feed ingredients derived from their use on animals. The order defines the procedure for state registration of genetically modified organisms sources of feed, feed additives and veterinary medicinal products containing genetically modified organisms or derived from their use.The basic components and objectives of the integrated system of state monitoring of GMO sources of feed, feed additives, premixes for livestock and poultry. The basic methodological approaches and criteria for the development and implementation in Ukraine of the State monitoring of feed containing GMO sources including requirements for sampling, setting the frequency of sampling and analysis of risks at all stages of the chain «production – consumption» . Based on the analysis of a draft list of feeds, feed raw materials and premixes that must be controlled on content of genetically modified organisms.
CLINICAL SIGNS IN DIAGNOSTIC OF CIRCOVIRUS INFECTION IN PIGS
Authors: Novitska O.
Published: October 2016

Clinical signs the disease associated with porcine circovirus (PCVAD) imitate many other diseases (classical swine fever, transmissible gastroenteritis, porcine parvovirus, pseudo rabies (Aujeszky’s disease), leptospirosis et al.), which complicates diagnosis of the disease in the early stages of infection among livestock. Late diagnosis, lack of treatment and high price vaccines contribute to the formation of stationary fire PCVD. Therefore, the preliminary diagnosis based on clinical signs and pathological changes in animals suffering from PCV2, is the primary in fight PCVD. According the notification of clinical and pathological signs PCVD we examined the number of pigs on the farm that specializes in young animals received and fattening. Farms specialists concerned about the low increase of body weight, but they did not think of circovirus infection of pigs. We divided all pigs in to three groups. The first group – adult sows, the second group – pigs 0 – 28 days old, the third group – pigs between 35 – 180 days old. All animals were clinically examined. Animals of the first group did not have clinical signs specific to PCVD. Animals of the second group were characterized with heterogeneity of group, weight of piglets at birth ranged from 0.9 to 1.3 kg. Animals were behind in growth, development, mortality ranged from 10%. Diarrhea and cough were not detected. The third group of animals was characterized with strong heterogeneity of herds and low growth. Testing revealed a lag in growth and development, depression, palemucous membranes, increasing the inguinal, submaxillary, retropharyngeal lymphnodes. Autopsy revealed: fibrinous pleuropneumonia, myocarditis, filling with blood of spleen and liver, enlarged kidneys, increased all the lymph nodes, especially in guinal and ripples. Signs of generalized staph infection were recorded with one pig. Using PCR analysis confirmed PCV2.
БРОНХОПНЕВМОНІЯ ТЕЛЯТ: ДІАГНОСТИКА ТА КОМПЛЕКСНА ТЕРАПІЯ
Authors: Slivinska L. , Demydjuk S. , Shcherbatyy А. , Mazurok P.
Published: October 2016

The results of nonspecific resistance indices, hematological, biochemical research on complex treatment of calves suffering from bronchopneumonia. It is established that the use of antibiotics (intramitsynu, tsefatoksymu and betamoksu LA), drugs that regulate the acid–base status, provide therapeutic efficacy, respectively – 75, 94 and 88% and shorten the duration of their treatment in 7 – 12 days. The treatment had a positive impact on the overall condition of the animals. In the blood have established probable increase in the number of red blood cells, respectively, 23.2% (p ˂ 0.001); 36.6 (p ˂ 0.001); 29.5% (p ˂ 0.05), hemoglobin contents to 8.04 (p ˂ 0.05); 17.3 (p ˂ 0.001) and 17.5% (p ˂ 0.001), reducing the number of leukocytes (p ˂ 0.001) according to 31.8; 34.3; 36.6%. The content of total protein in serum of calves experimental groups increased to 3.75 (p ˂ 0,001); 6.38 (p ˂ 0.05) and 6.93% (p ˂ 0.001), respectively, due to increased albumin fraction in the second experimental group 18.4% (p ˂ 0.001), the third – 14.8% (p ˂ 0,01). The results showed that the applied treatment had a positive impact on the carotene content in blood serum (to 43.5; 34.6; 46.0%; p ˂ 0.05), total calcium (respectively 15.0, 14.7; p ˂ 0.05; 18.3%; p ˂ 0.05) and inorganic phosphorus (6.4; 15.3; 15.8%; p ˂ 0.05).Integrated treatment of calves suffering from bluetongue bronchopneumonia, helped indexes increase non–specific resistance. The data obtained by the use of antibiotics (intramitsynu, tsefatoksymu and betamoksu LA) promoted faster normalization lizotsymnoyi and bactericidal activity of blood. Thus, in the first experimental group LASK increased by 25.6% (p ˂ 0.001); the second – by 26.3% (p ˂ 0.001) and the third at 35.3% (p ˂ 0.001). BASK calves tended to increase according to 11.7; 10.6 and 11.3%.
ФУНКЦІОНУВАННЯ ІМУННОГО БАР'ЄРУ КИШЕЧНИКА ПОРОСЯТ ЗА ДІЇ ТЕХНОЛОГІЧНОГО СТРЕСУ
Authors: Stojanovskyj V.G. , Ogrodnyk M.T.
Published: October 2016

The article presents the results of the study of the functional state structures intestinal immune piglets growing industrial and post–action process stress–weaning. It was established that the small intestine of piglets before and after weaning operates a big yeyuno–ilealPeyer’s patche and small Peyer’s patches. The structure of yeyuno–ileal Peyer’s patche of pigs 28–day age (weaning) at the macroscopic level allocated separate nodules that are intensely stained and clearly different, especially in the caudal part that can point to a marked reactivity of lymphoid tissue that forms their basis. At the stage of resistance (30 days after weaning) in the structure of yeyuno–ileal Peyer’s patche of pigs at the macroscopic level dome–shaped nodules are tight, so no free fields are not registered. At the histological level ofyeyuno–ileal Peyer’s patche of piglets before weaning registered primary and secondary nodules with reactive centers of reproduction, which is a sign of complete morphofunctional maturity lymphoid tissue, whereas when the piglets after weaning secondary nodules turned out far less, some of them have clearly formed shell, which may indicate the inhibition of immune responses within mucosal lymphoid tissue of the gastrointestinal tract or the development of immunodeficiency state of cellular immunity as a result of the stress of weaning.
АЛЕЛЬНИЙ ПОЛІМОРФІЗМ ГЕНА BOLA–DRB3.2 В ЗВ’ЯЗКУ З ТИПАМИ ВИВІДНОЇ СИСТЕМИ ВИМЕНІ ТА МАСТИТАМИ КОРІВ УКРАЇНСЬКОЇ ЧОРНО–РЯБОЇ МОЛОЧНОЇ ПОРОДИ
Authors: Suprovych T. , Suprovych N. , Karchevska T. , Chornyy I. , Chepurna V.
Published: October 2016

The results comparing the two types of markers associated with susceptibility (by resistance) cows to mastitis presented in the article. Previous studies indicated 2 markers. There are allele gene BOLA–DRB3.2 and placer or magistral type of excretory ducts udder. The study was conducted by identifying spectrum allele's and types of excretory ducts udder of cows in 47 Ukrainian Black Pied dairy breed. Some of the animals (28 caw) have in the genotype «informative» alleles that indicate association with mastitis or resistance to the disease. Susceptible cows to the disease often have a placer type (half of the animals). Healthy cows have oftener magistral type of main excretory system (35.7%). With magistral type have genotype 9 cows «positive» alleles (32.1%); 11 animals with placer type have only «negative»alleles (39.3%). Comparing the presence of two types of markers in healthy and susceptible animals found two predominant choices:– healthy animals have the oftener magistral type of excretory system of the udder, and at least one allele associated with resistance to mastitis (9 cases);– in diseased animals have the oftener placer type of excretory system and at least one allele associated with susceptibility to mastitis (11 cases).The high incidence of these options indicates that there are natural connections between the type of excretory ducts udder, by alleles BoLA–DRB3.2 and mastitis.
ОСОБЛИВОСТІ ГЕМАТОЛОГІЧНОГО І БІЛКОВОГО ПРОФІЛЮ ТА СТАНУ НЕСПЕЦИФІЧНОЇ РЕЗИСТЕНТНОСТІ ОРГАНІЗМУ ПТИЦІ ПРИ ЗАСТОСУВАННІ ПРОБІОТИКА ТА НАНОКОБАЛЬТУ
Authors: Turko Ya. , Ushkalov V.
Published: October 2016

The obtained results on the state of the immune system in conjunction with these dynamics oferythrocyte number and total hemoglobin, changes in protein gramme convincingly testify adaptogenic effect of probiotics and cobalt nanoparticles at a dose of 0.08 mg/kg of body weight on the state of nonspecific resistance and protein metabolism in the body of the research poultry.However alimentary chronic entering of probiotic togetherwith cobalt nanoparticles at a dose of 0.80 mg/kg at the late terms of research leads to such changes in protein grammewhich are typical signs of protein synthetic liver function (with hypoalbuminemia) and the development of immunosuppression (for increased levels of seromucoid).According to results of chronic toxicological experiment on laying hensis possible to assert about no harmful actions of probiotics and probiotic along with the addition of cobalt in the form of salts and nanoparticles at a dose of 0.08 mg/kg of body weight, which makes it really as biotic.Adaptogenic effect of probiotic preparations and additives of cobalt nanoparticles at a dose of 0.08 mg/kg of body weight at alimentary earnings to hens body consists in strengthening of immune reactions along with the restoration parameters of erythropoiesis and protein exchange.
ВПЛИВ ФЕРРОВЕТУ 7,5 % І ФЕРОСЕЛУ Т НА КОНЦЕНТРАЦІЮ МІНЕРАЛЬНИХ РЕЧОВИН В СИРОВАТЦІ КРОВІ ПОРОСЯТ, ХВОРИХ НА ФЕРУМДЕФІЦИТНУ АНЕМІЮ
Authors: Todoriuk V.B. , Gutyj B.V. , Khomyk R.I. , Vasiv R.O.
Published: October 2016

In the article presents the results of experimental studies on the pharmacological action of iron dextran preparations – ferrovet–7.5%, which in 1 ml dextran containing 75 mg of ferric iron, and ferosel–T, 1 ml of which, in addition to 75 mg of ferric iron, additionally contains 0.3 mg selenium (in the form of sodium selenite) the concentration of minerals in the blood serum of pigs for latent iron deficit anemia.Established that at 21 days postnatal period, in the whey of blood of piglings for latent iron deficit anemia, a level of Calcium was on 11.8%, and to Phosphorus – on to 8.4% lower. Reserves of iron in the indicated period of depot were fully used for hematopoietic and receipt of its mother's milk insufficient for optimal erytrotsytopoez because the concentration of iron in the blood serum of pigs did not satisfy his need for hematopoiesis.In the case of iron dextran preparations for the treatment of iron deficiency anemia pigs found an increase in Calcium, Phosphorus, Selenium and Iron. So on 21 days after birth for piglings, which entered ferrovet 7.5%, in the whey of blood the concentration of Calcium was increased on 30.0% (r < 0.01), to Phosphorus – on 15.6%, and which ferosel–T was entered accordingly increased on 33.4% (r < 0.01) and 19.3%.The concentration of Selenium and Iron in the blood serum of piglets research groups, which used ferrovet 7.5% and ferosel–T is likely to have increased since 7 day experiment. Shown that iron deficit anemia of piglings efficiency of application of preparation of ferosel–T with a medical and prophylactic purpose is higher, than to preparation of ferrovet–7.5 %, that predefined by the combined action of Iron and Selenium.
ЧУТЛИВІСТЬ ЗБУДНИКА КИШКОВОГО ІЄРСИНІОЗУ ДО ДЕЗІНФЕКТАНТІВ
Authors: Kozlovska G.V.
Published: October 2016

The article presents the results of a study of the inactivating action of modern disinfectants pathogen intestinal yersiniosis. Determine the sensitivity of the laboratory and field strains of Yersinia enterocolitica to drugs «Virosan» and «Biokontakt». Preparations intended for preventive disinfection of livestock buildings and facilities subject to veterinary supervision, items of artificial insemination, filling dezbarerov, as well as for disinfection of transport, processing of hatching eggs. As a result, three–time experiments conducted in similar conditions found that the drugs «Virosan» and «Biokontakt» are effective disinfectants in the case of pathogen Yersinia enterocolitica contamination of various objects (concrete, brick, wood and tile, metal). The studies found effective concentrations mentioned disinfectants and the exposure determined under what strains of Y. enterocolitica is completely inactivated. It was established that the preparation «Virosan» at a concentration of 0.1% has a bactericidal effect at 30 minutes, at a concentration of 0.2–0.5% – within 15 minutes. It is also found that the disinfectant «Biokontakt» ensures complete killing Y. enterocolitica in all test sites at a concentration of 0.01% within 30 minutes, at a concentration of 0.1 – 0.5% – bactericidal effects is apparent within 15 min. Differences in their antibacterial action on laboratory and field strains of the pathogen were observed.
ПАТОГІСТОЛОГІЧНА ХАРАКТЕРИСТИКА ІМУННИХ ОРГАНІВ КУРЕЙ–БРОЙЛЕРІВ ПРИ СПОНТАННОМУ ОРНІТОБАКТЕРІОЗІ
Authors: Blishch G.I. , Kotsyumbas G.I.
Published: October 2016

The article presents the results of pathological, histopathological and histochemical studies of the immune system organs of broilers (in particular, the bursa of Fabricius, thymus and spleen) in conditions of ornithobacterium infection. The obtained results were conducted on the morphological level which helped to identify the fact that in the case of ornithobacterium infection the atrophy of immune structures is taking place; decompensation of different cells involved in the immune response indicates the development of immunosuppressive conditions. The analysis of bacteriological and serological studies of pathological material taken from sick chickens at the poultry farms in Lviv region, Ukraine, led to identifying the disease as ornithobacterium infection. During postmortem autopsy of 30 broilers of 28 days of age, the fragments of the thymus, spleen and the bursa of Fabricius were selected, which were then put into the 10% formaldehyde solution and Carnoy’s solution. Dissections were conducted via the MC–2 microtome and stained with hematoxylin–eosin using McManus methods. Histological research of the immune organs of broilers led to the following findings: an atrophy of lymphoid follicles located in the bursa of Fabricius which resulted in a sharp narrowing of the cortical area due to the reduced number of B–lymphocytes; plasma cells and thickening of the interlobular connective tissue; congestion in the thymus, loose filling of the cortical area with the small lymphocytes and an increase of the immature forms in it as well as a significant increase in the number of Hassall’s corpuscles in the cerebrospinal fluid; atrophy of the lymphoid tissue in spleen due to a sharp decrease in the number of T–lymphocytes in the periarterial lymphoid couplings, destruction of B dependent areas, and reduced number of micro– and macrophages in the red pulp. Immunosuppressive changes in the spleen were accompanied by the violation of vascular permeability, permeation of PAS positive and pyroninophylic proteins into the vessels and reticular foundations of the periellipsoid and lymphoid couplings and intravascular coagulation which manifested itself on the morphological level by forming fibril structures and blood clots in the lumen of blood vessels.
ВПЛИВ КОМПЛЕКСНОГО ЛІКУВАННЯ ТЕЛЯТ НА ОБМІН МЕТАЛОПРОТЕЇНІВ ТА АКТИВНІСТЬ ФЕРМЕНТІВ СИСТЕМИ АНТИОКСИДАНТНОГО ЗАХИСТУ ЗА ГІПОПЛАСТИЧНОЇ АНЕМІЇ
Authors: Zhukovskyy I.K. , Slivinska L.G. , Levchenko V.I.
Published: October 2016

The research results of calves suffering from hypo plastic anemia of iron content, enzyme activity of antioxidant protection (AOP) and the exchange metal protein are listed in the paper.Trace element ration adjustments of calves were performed by including premixes scarce trace elements, which were in the form of sulphates ME (the first experimental group) and methionine chelate compounds (the second experimental group).It is established that the complex treatment of calves eliminates the major clinical symptoms of iron deficiency, normalizes metal enzyme activity of various components of the system AOP. In particular at the end of the experiment, the concentration of iron significantly (p < 0,001) was increased at 1.9 and 2.2 times, the haptoglobin level of was respectively higher at 47.1% and 49.3% compared to the beginning of the experiment.The malonic dialdehyde concentration in serum of calves of the first group decreased by 20.3% (p < 0.001) in the second group, where chelate compounds were applied – by 29.8% (p <0.001).As a result of the treatment the hemoenzyme activity increased: catalases by 31.2% and 36,8% (p < 0,001), peroxidase by 37,6% (p < 0.01) and 49.2% (p < 0.001) and cytochrome oxidase by 54.7% (p <0.01) and 86.5% (p < 0.001).Determination of investigated metal protein can be informative diagnostic criteria for metabolic disorders of calves suffering from hypo plastic anemia. The best therapeutic effect in the treatment of calves of the second group is made, which got minerals in the form of chelate compounds.
ПОШИРЕННЯ І ХАРАКТЕРИСТИКА СЕРЦЕВИХ ШУМІВ У СПОРТИВНИХ КОНЕЙ
Authors: Maksymovych I.A.
Published: October 2016

The paper presents the research results dissemination heart murmurs among sport horses and their impact on the performance of the animals. The studies were conducted on horses Ukrainian, Hanoverian and Westphalian warmblood and English thoroughbred breed used in classical equestrian sport.The clinical studies included measurements of body temperature, counting pulse rate and breathing, rate, cardiac shock, cardiac tones and murmurs. The criteria for evaluation of heart murmurs were: time of appearance, location, intensity, belonging to the cardiac phase (systole or diastole). Heart murmurs evaluated on a scale of 1 to 6. The studies were conducted at rest and not earlier than 2–hours after exercise.Heart sounds were recorded in 64% of sport horses, but most of them had no clinical significance since only 6.7% of the studied animals was reduced performance. Most recorded systolic murmurs (mitral valve – 20% of horses, tricuspid valve – 24%, pulmonary artery – 8%, aortic valve – 4% of the animals), rarely diastolic (aortic valve – 8% of horses).Our studies suggest that the prevalence of heart murmurs in sport horses depends on the age of the animals, exercise, functional and morphological changes valvular heart caused by constant exposure to sports training and more. However, to confirm these findings required further investigation.
ТЕХНОЛОГІЯ ВИРОБНИЦТВА ВАРЕНИХ КОВБАС З ДОДАВАННЯМ ЙОДОВАНОЇ СОЛІ ТА ЇЇ ВЕТЕРИНАРНО–САНІТАРНА ЕКСПЕРТИЗА
Authors: Farionik T.V.
Published: October 2016

Iodine deficiency in the biosphere, mainly in the soil, leading to the development of endemic goiter and other iodine deficiency disorders. As shown by numerous studies of this disease tend to over 1 billion world population. Endemic goiter and much of the territory of Ukraine, including the Vinnitsa region. Presented current research study of recipes and technologies on system cooked sausages.Currently boiled sausage enjoys fairly high demand among the population, due to the fact that a large for most of the population gives it an advantage.Innovation activity represents one of the most effective directions.It should facilitate the development of innovative activity that promotes penetration into new markets, growth in production.Today the most important and effective innovative organizational and technical measures introduced in sausage production can be considered are:–The use of new recipes for manufacturing products;– Installation of new equipment, which can significantly reduce the cost of manual labor and power inputs, and improve product quality and reduce the share of marriage. Sausage production is seen as thermochemical method of preserving meat products. For each type of sausages set a production process approved technological instructions and recipes.Strict adherence to recipes and technological instructions and sanitary regime over the course of the process is a prerequisite for obtaining high–quality sausages.Crucial in the production of sausages is raw. The main raw materials are beef and pork. Occasionally use lamb, horsemeat.Equally important is the creation of a new generation of products that have general strengthening and preventive action. The components of these products can protect the body from the harmful effects of the environment and the emergence in the human diseased cells. The constant lack of iodine leads, first, to reduce the synthesis and secretion of primary thyroid hormone – thyroxine. The role of thyroid hormones in the body is extremely high, most of the vital functions performed by their participation. The main physiological effects of thyroid hormone is to stimulate synthesis, growth, development and differentiation of tissues.Developed products to include microorganisms that can synthesize biologically active structures (antibodies, receptors, hormones) that contribute to the removal or destruction of harmful decay and systems, thereby prevents human disease.
ЧУТЛИВІСТЬ ЗБУДНИКІВ БАКТЕРІАЛЬНИХ ХВОРОБ ПТИЦІ ДО АНТИБАКТЕРІАЛЬНИХ ПРЕПАРАТІВ
Authors: Fotina A.A. , Klischova Zh.E.
Published: October 2016

The using of antibiotics and antimicrobials drugs without control may leads to the development of numerous complications and resistance of microorganisms to antibiotics. The using of antibiotics and antimicrobials drugs should are controlled on poultry farms. That is why the monitoring and determination of sensitivity of bacterial diseases agents to antimicrobial drugs are very important. Results of salmonellas’ and kolibakterias’ monitoring in poultry’s farms of Ukraine are introduced in the article. Researches were conducted at the Department of veterinary sanitary examination, microbiology, zoohygiene and safety and quality of livestock products of Sumy NAU. Sampling for microbiological studies was conducted from the hatchery and from pathological material and premises where poultry of different age groups was held. The spread of the disease, morbidity, mortality, mortality rate, age characteristics, economic loss what diseases cause to the poultry farms were counted. Identification of Salmonella and Escherichia was conducted by ELISA with using of RIDASCREEN® and LOCATE® test systems, according to methodical recommendations of RIDASCREEN® and LOCATE® test systems using. The results were read visually or after addition of storageco with ELISA–photometer (reader) at 450 nm. Sensitivity to antibiotics was determined by disco – diffusion method in agar. Microbiological monitoring of a number of poultry farms in Ukraine has shown that agents of bacterial diseases’ are widely spread. Between the isolated microflora largest number were accounted for Salmonella (54.1%) and the Escherichia (30.8 per cent). The rest (15,1%) were isolated cultures of Proteus, Pseudomonas, Klebsiella, Salmonella, Campylobacteria, Enterobacteria, and Clostridia Citrobacter. This indicates that systematic control over the availability of the causative agents of bacterial infections in all critical points of production of poultry products is very necessary. Among isolates that were isolated from ill poultry and poultry objects, differences in their sensitivity to antimicrobial agents from active substances that officially have registered in our country were discovered. Bactericidal activity of relatively isolated cultures was showed by colistin, ftorfenicol, zeftiocur, TimTil 250, doxicyclin, enroxil and sarafloxacin.
КОНТРОЛЬ ЛІПОФІЛЬНИХ ТОКСИКАНТІВ У МОЛОЦІ З ВИКОРИСТАННЯМ МІКРОБІОЛОГІЧНОГО ЕКСПРЕС–МЕТОДУ
Authors: Khimich M.S. , Gorobey O.M. , Salata V.Z.
Published: October 2016

In recent years, Ukraine is constantly increasing livestock production. But at the same time, in many modern industries, veterinary and sanitary control of food is on insufficient or non–existent. In this connection the trading network can get dangerous foods for consumers.Today in the world there are numerous number of methods of analysis of food safety (spectrometry, photometry, chromatography, etc.). But in our country, a network of specialized laboratories which would be able to provide high–quality and objective analysis of the food is still very limited.It is also important to remember that the current chemical–analytical methods unfortunately unable to answer the general reaction of the organism to influence the recipient of the object, which is associated with several factors: the test object may contain toxicants with content during the study not predicted poorly understood substances, and importantly, is not considered character combined action of toxicants. That despite these shortcomings and remains an important method of determining the toxicity bioassay.The aim of our research was to establish the feasibility and effectiveness of the rapid microbiological method using ciliates Colpoda steinii to determine the toxicity of skimmed milk.Toxicity studies were carried out in parallel by three methods: bioassay in laboratory animals (white mice) and microbiological using as a test organism ciliates Tetrachimena piriformis and Colpoda steinii.The proposed method of using as a test organism ciliates Colpoda steinii, including the preparation of test culture ciliates Colpoda steinii, sampling investigational product, extracting samples of chemically pure acetone, filtering selected extract dilution leachate solution rod–Lozinski, introducing the filtrate into a test culture ciliates, temperature control resulting mixture at a temperature of +26 ... + 28 °C and determine the toxicity of the investigational product, observing the vital functions of ciliates during incubation.The research found that data on the toxicity of skimmed milk a certain prescribed manner identical data obtained using classical methods.Established that the strong toxicity of skimmed milk in the application of research results ciliates C. steinii get already at 3min. And weak toxicity or netoksychnosti – 1 hour.
ЛАБОРАТОРНА ХАРАКТЕРИСТИКА РІДКОГО ЕНЗИМНОГО МИЙНОГО ЗАСОБУ «ЕНЗИМИЙ» ДЛЯ САНОБРОБКИ У МОЛОЧНІЙ ПРОМИСЛОВОСТІ
Authors: Shynkaruk O.Y.
Published: October 2016

We have developed a liquid enzyme detergent «Enzymyi» for sanitization of CIP–installations on milk plants that includes potassium hydroxide, complexones, proteolytic enzyme, stabilizers, and distilled water.The previous laboratory studies showed that the peak activity of «Enzymyi» on milk proteins was observed at as high as 65.8% at 60°C. Its activity decrease with the rise of temperature above 60 °C due to denaturation of enzymes since the heat causes changes in the primary structure and conformation of enzyme. It is also established that at pH solutions of 8.35 units proteolytic activity increased 1.7 times (P ≤ 0.01) compared to 7.2 pH units and reached its maximum activity 65.8 – 64%. Further increase of pH solutions from 8.35 units to 8.6 units contributed to gradual inhibition of enzymes resulting in reduction of proteolytic activity to 0. Proteolytic activity of the studied sample of enzyme detergent at water hardness of 0.357 mg–eq/l also reached the maximum of 60.5% and at increasing water hardness from 0.714 to 1.071 mg– eq/l PA decreased by 25.4% (P ≤ 0,05), i.e. with increasing water hardness PA reduced respectively. The article presents the study results as regards foam generating ability, foam stability and surface tension of solutions. The liquid detergent enzyme «Enzymyi» in concentrations of 0.03; 0.05; 0.07; 0.1; 0.3; 0.5; 0.7; 1% at the temperatures 20 °C and 60 °C was used for study. The article presents the study results as regards foam generating ability, foam stability and surface tension of solutions. The liquid detergent enzyme «Enzymyi» in concentrations of 0.03; 0.05; 0.07; 0.1; 0.3; 0.5; 0.7; 1% at the temperatures 20 °C and 60 °C was used for study.The results showed that «Enzymyi» practically doesn’t develop foam generating ability at the concentration from 0.03 to 1%. Foam stability does not exceed the standard, thus no more than 50% of the solution in 5 minutes that allows using the available composition for circulation washing of dairy equipment without its dismounting.
ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ ТА АКТИВНІСТЬ СИСТЕМИ АНТИОКСИДАНТНОГО ЗАХИСТУ У КРОВІ КОРОПІВ ЗА ДІЇ РІЗНОГО РІВНЯ ВІТАМІНУ Е І СЕЛЕНУ В РАЦІОНІ
Authors: Yurchak S.V. , Derenj O.V. , Vishchur O.I. , Zabytivskyi Yu.M.
Published: October 2016

The article consist data about effect of different levels of vitamin E and selenium in the diet of carps during their growing and also informationabout the influence on processes of lipid peroxidation and activity of antioxidant protection in their body.The experiment conducted in three experimental ponds. After wintering there were placed four ears mature females and males carp, six individuals in each group. Supplements of vitamin E administered at a rate of 25 mg/kg and drug «Sel–Plex» the rate of selenium – 0.3 mg/kg were added to further basic diet(BD) of female and male carp first experimental group (EG1). The second experimental group (EG2) received (BD) and vitamin E supplements in an amount of 75 mg / kg, and just as in EG1 – selenium – 0.3 mg/kg of feed.The control group received fish feed without additives vitamins and minerals.Feeding lasted for 30 days, due to the physiological needs of the fish feed. After spawning in fish of research and control group swere taken blood samples for biochemical research.The study led to a dose–dependent decrease (p < 0.01 – 0.001) content of TBA–active products and hydroperoxidase of lipids, but did not significantly effect onsuperoxide dismutase and glutathione peroxidase activity of blood So, the reduction of lipid peroxidation products in the carp’s blood of experimental groups probably was caused by the growth of non–enzymatic level, which is associated with fat–soluble vitamins.
ВЛАСТИВОСТІ SALMONELLA TYPHI ЗА ДІЇ ЕЛЕКТРОМАГНІТНОГО ВИПРОМІНЮВАННЯ НАДВИСОКОЇ ЧАСТОТИ
Authors: Yablonska O.V. , Mekh N.Y. , Duhnich T.A.
Published: October 2016

In Ukraine, a role in the etiology of Salmonella enteric disease in humans and animals is important because there are changes in the structure of these pathogens serovariantic who skillfully adapted to the environmental conditions. Without studying the biological properties of pathogens, their accounting is impossible to predict the development of epidemiological and epizootic processes and to develop effective measures regarding the reduction of intestinal infections.Electromagnetic radiation of ultra–high frequency (UHF) is widely used in everyday life and medicine and more recently in veterinary medicine, and the effect of microwaves on the cells and the organism as a whole is the subject of scrutiny. Development of optimal microwave heating modes based on biophysical laws of microwave radiation with cells and the mechanism of absorption of microwave energy cell suspensions.Curing agar and broth cultures of Salmonella in the microwave destroys them after 30 seconds.Therefore, we believe that the electromagnetic radiation of the microwave oven when it is exposed for at least 30 seconds. It can be used for the destruction of enteropathogenic microorganisms in different substrates.
FREQUENCY OF DEA 1.1 ANTIGEN IN GERMAN SHEPHERDS
Authors: Milczak A. , Abramowicz B. , Madany J. , Winiarczyk D. , Wrześniewska K. , Bochyńska D. , Sahinduran S.
Published: October 2016

Objectives: A correlation between blood type and the incidence of certain diseases in humans was established many years ago. It may be assumed that similar correlations could occur in dogs. Therefore, establishing the geographical and breed distribution of erythrocyte surface antigens in dogs may be of academic interest and useful in practice too. The aim of the study was the assessment of DEA 1.1 frequency among German Shepherds in the Lublin region of Poland.Material and methods: Our study involved 20 German Shepherds from Eastern Poland. The presence of blood antigen was detected using a quick immunochromatographic test. In 9 cases a donor–recipient compatibility test was also performed.Results: The antigen DEA 1.1 was detected in 15% of dogs. A clear, macroscopically visible agglutination did not occur in any of the donor–recipient compatibility tests.Conclusion: The breed studied may therefore be recognised as a suitable blood donor.
ORGANIC TRACE ELEMENT COMPLEX FOR CALVES FEEDING
Authors: Radchikov V.F. , Tsai V.P. , Gurin V.K. , Lyundishev V.A.
Published: October 2016

Trace elements are commonly used in the form of inorganic salts poorly digested by ruminants due rumen microflora, which transits its greater part into indigestible and insoluble form. Trace elements in the form of chelates are supplied with amino acids and peptides, and used by the body much more efficiently. Inclusion of organic trace element complex (OMEK) in compound feed KR–1 in the amount of 10% of the existing standards for trace elements content in typical formulations for growing young cattle for meat has a positive impact on palatability of feeds, morphological and biochemical composition of blood and animals’ performance, economic efficiency of calves fattening. Use of organic trace element complex in compound feeds KR–1 increases concentration of total protein in blood by 8 – 12% (P < 0.05), reduces the amount of urea by 11 – 16% (P < 0.05). Comparative analysis showed that animals in experimental group II used the feeds more efficiently. Metabolizable energy consumption per 1 kg of weight gain made 33.7 MJ against 37.4 MJ in the control group or by 9.9% lower, the same trend is established for digestible protein cost – by 10%. Inclusion of OMEK in compound feed KR–1 for young cattle ensures average daily weight gain increase by 12.3% (P < 0.05), decreased of feed cost per 1 kg of weight gain by 10%. Use of organic trace element complex helps to decrease the cost price of weight gain, by 9.0% and obtain extra profit of $37.2 USD per one animal during the experimental period.