bluuebunny/crossref_metadata_2025 huggingface dataset:
Participation
Authors: Holert Tom
Published: June 2023

Aiming at rendering “somewhat irrelevant” the “question of sadism” in the critique of film’s “seductive epistemological promise,” Leo Bersani and Ulysse Dutoit argued for a “participation in the film” that is “at once self-reflexive and self-shattering.” Such participation may bring about the very panhuman condition of “being-togetherness” that Bersani and Dutoit have registered in the ontology of Alain Resnais’s and Terrence Malick’s films. This peculiar “community of all being” is based on a specific (and paradoxical) kind of movement within the spatiality of a remembrance engendered by the inhibition of movement. Certain films thus render possible connection and participation due to the mobility they entail.
Perhaps
Authors: Redrobe Karen
Published: June 2023

This essay explores Leo Bersani’s use of intellectual uncertainty, openness, and tautness in his writing to create a space for thinking about “what is politically unfixable in the human” (Homos 71). In particular, this essay, which travels under the banner of the word perhaps, pays attention to Bersani’s first brief, then extended response to Jean Genet’s statement (in the context of his play, The Maids), “I suppose that there is a union for domestic servants—that is not our affair.”
Renunciation
Authors: Dohoney Ryan
Published: June 2023

In their ruminations on Mark Rothko’s Chapel paintings, Leo Bersani and Ulysse Dutoit posit a mode of aesthetic consciousness produced by acts of renunciation. Rothko deliberately relinquishes his paintings’ visibility and thus, like Beckett and Resnais, impoverishes a primary feature of his artistic medium. Bersani and Dutoit’s concept of renunciation undermines modernist medium specificity and points to a form of pseudomorphosis that reveals the fundamental homo-ness between the arts. This speculative essay builds on their argument that such renunciative gestures produce aesthetic models of nondual consciousness and brings Bersani’s later writings into conversation with musical experience. In Bersani’s encounter with Proust and music, musical experience proffers a powerful ego solvent in favor of ontological interpenetration. An attention to music—here, that of Morton Feldman and Joan La Barbara—avers the power of modernism to shatter the modern ego.
Seductive
Authors: Litvak Joseph
Published: June 2023

Leo Bersani was an enormously seductive critic, and he frequently wrote about seductiveness, beginning with his first book, Marcel Proust: The Fictions of Life and Art. This essay focuses on a passage in that book where Bersani discusses Proust’s character, the Baron de Charlus, whose seductive orientation toward the world Bersani relates to Charlus’s “homosexual talk.” This essay considers the fate of seductiveness within Bersani’s own career-long homosexual talk. It moves, by way of his queer provocations of the 1980s and 1990s, from his first book to his 2013 preface to that book, and shows how, even as Bersani reformulated the seductive in increasingly austere terms, he continued to deploy it, never forgetting how close it is to the intolerable.
Un/reading
Authors: Chaudhary Zahid R. , Wiegman Robyn
Published: June 2023

Responding to the theme of this special issue’s engagement with the work of Leo Bersani, “Un/reading” tracks the itinerary of a single quotation across the four appearances it makes in published work between 2011 and 2018. While it is easy to query whether Bersani was aware of the citational redundancy, this essay focuses on the pedagogy of reading it suggests, not only in terms of Bersani’s own thought but for the critical humanities more generally. The quote in question posits the absolute necessity and impossibility of psychoanalysis in its attempt to “theorize an untheorizable psyche.” By focusing on the role that repetition plays in Bersani’s work, including his interest in nondialectical understandings of contradiction, this essay studies the elegant and cogent model of reading found in Bersani’s work in order to value the critical intuitions he repeated in order to hone.
Withdrawal
Authors: Young Damon Ross
Published: June 2023

Leo Bersani’s writings on aesthetics, often coauthored with Ulysse Dutoit, repeatedly deploy the trope of withdrawal, finding in the aesthetic works they discuss, such as Jean-Luc Godard’s film Contempt, a paradoxical double movement of bringing into view while projecting the erasure of form and distinction. In their reading of Contempt, Bersani and Dutoit adumbrate a uniformity that would depend on the elimination of measurement, reference, and subject-object relations. In doing so, they transfigure the idea of antirelationality often attributed to Bersani, associating the death it surely names with the illumination of Being.
Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP
Authors: Ustinova Kseniya , Ruhnow Felix , Gili Maria , Surrey Thomas
Published: June 2023

Mitotic spindle assembly during cell division is a highly regulated process. Ran-GTP produced around chromosomes controls the activity of a multitude of spindle assembly factors by releasing them from inhibitory interaction with importins. A major consequence of Ran-GTP regulation is the local stimulation of branched microtubule nucleation around chromosomes, which is mediated by the augmin complex (composed of the eight subunits HAUS1-HAUS8), a process that is crucially important for correct spindle assembly. However, augmin is not known to be a direct target of the Ran-GTP pathway, raising the question of how its activity is controlled. Here, we present the in vitro reconstitution of Ran-GTP-regulated microtubule binding of the human augmin complex. We demonstrate that importins directly bind to augmin, which prevents augmin from binding to microtubules. Ran-GTP relieves this inhibition. Therefore, the augmin complex is a direct target of the Ran-GTP pathway, suggesting that branching microtubule nucleation is directly regulated by the Ran-GTP gradient around chromosomes in dividing cells.
Decision rules for egg-color-based rejection by two cavity-nesting hosts of the brown-headed cowbird
Authors: Di Giovanni Alexander J. , Villa Juliana , Stanback Mark T. , Thompson Charles F. , Sakaluk Scott K. , Hauber Mark E. , Hanley Daniel
Published: June 2023

Hosts of obligate avian brood parasites often evolve defense mechanisms to avoid rearing unrelated young. One common defense is egg rejection, for which hosts often rely on eggshell color. Most research has assumed that hosts respond to perceived color differences between their own eggs and parasite eggs regardless of the particular color; however, recent experiments have found that many hosts respond more strongly to brown foreign eggs than to equally dissimilar blue eggs. Yet, none of these prior studies tested a brown-egg-laying species and, with only one exception, all were conducted in open nests where light levels are considered sufficient for effective color-based egg discrimination. Here, we explored how two cavity-nesting hosts of the parasitic brown-headed cowbird (Molothrus ater) – the blue-egg-laying eastern bluebird (Sialia sialis) and the brown-egg-laying house wren (Troglodytes aedon) – respond to experimental eggs painted six distinct colors ranging from blue to brown. Rejection responses of both hosts were best predicted by perceived differences in color between the model egg and their own eggs. Specifically, we found that house wrens preferentially rejected eggs bluer than their own eggs. However, although we found that bluebirds relied on perceived differences in color for their egg rejection decisions, further tests are needed to determine whether they preferentially rejected brown eggs or simply responded to absolute perceived differences in color. These findings demonstrate that these cavity-nesting birds treat perceived color differences in distinct ways, which has important implications on the coevolutionary arms races and the interpretation of avian-perceived color differences.
Nickel-Catalyzed Selective Incorporation of Isolated In-Chain Carbonyls into Ethylene/Carbon Monoxide Copolymer Using Metal Carbonyls as a Carbonyl Source
Authors: Yonezaki Go , Seidel Falk William , Takahashi Kohei , Nozaki Kyoko
Published: June 2023

Non-alternating copolymers of ethylene and carbon monoxide were obtained by nickel-catalyzed polymerization of ethylene in the presence of Fe2(CO)9 as a carbon monoxide source. Higher selectivity to isolated ketone group than the previously reported nickel-catalyzed systems, while having a higher catalytic activity than the palladium catalysts in our previous report has been achieved. Furthermore, various P-stereogenic phosphine-phenolate ligands were developed to investigate the effect of ligand structure on the copolymerization. Finally, the degradability of the resulting polymer was evaluated.
Proceedings on cases of administrative offenses in courts of general jurisdiction
Authors: Schepalov Stanisav
Published: June 2023

The book examines the proceedings on cases of administrative offenses in courts of general jurisdiction as a scientific concept related to the legal procedural sciences. The author reveals the essence of the phenomenon he designates as an independent set of procedural relations, during which courts of general jurisdiction administer justice in administrative and tort cases. Its theoretical boundaries are defined and its relationship with other legal phenomena, including law, justice and judicial proceedings, is indicated. The general structure of this procedural activity is described.
For lawyers — scientists and practitioners, judges, teachers, students and postgraduates of law schools and anyone interested in this issue.
Predação de operárias de Ectatomma opaciventre Roger (Hymenoptera: Formicidae) sobre Hermetia illucens L. (Diptera: Stratiomyidae)
Authors: Moleiro Hugo Ribeiro , Giannotti Edilberto , Tofolo Viviane Cristina
Published: June 2023

Ectatomma opaciventre Roger é uma espécie de formiga cujas presas incluem espécies de moscas, no entanto, não se conhecia os comportamentos empregados pelas operárias em sua predação. Neste trabalho, foi elaborado um etograma da predação sobre larvas e adultos da mosca Hermetia illucens L. por operárias de E. opaciventre. Foram coletados indivíduos de três ninhos de E. opaciventre, sendo posteriormente transferidos para ninhos artificiais. Para cada um deles, foram oferecidas larvas e adultos vivos de moscas. A partir das observações, em um contexto de predação, os comportamentos observados que apresentavam funções semelhantes foram agrupados em categorias, com quatro delas tendo sido observados na predação sobre larvas e todas as oito na predação sobre adultos. As larvas não chegaram a ser predadas de fato (os comportamentos agressivos, realizados pelas formigas, aparentam estar relacionados à defesa do ninho), porém as moscas adultas serviram realmente como alimento.
Charlson comorbidity index to predict 28-day mortality in critically ill COVID-19 patients
Authors: Sugiarto Adhrie , Pryambodho  , Imelda Meilina , Aditianingsih Dita
Published: June 2023

BACKGROUND Severe COVID-19 patients may become critically ill and require treatment in the intensive care unit (ICU). As intensive care resources are limited, mortality predictors should be used to guide resource allocation. This study aimed to validate the Charlson comorbidity index (CCI) as the mortality predictor of critical COVID-19 patients in the ICU.

METHODS A retrospective cohort study was done in adult patients admitted to the ICU with severe COVID-19 at Cipto Mangunkusumo Hospital and Universitas Indonesia Hospital from March to August 2020. We extracted the subject’s CCI score from the medical records and the 28-day mortality after ICU admission. The CCI score was validated by the Hosmer–Lemeshow calibration test, determination of area under the curve (AUC), and optimal cut-off point for the critical patients in the ICU. We used the chi-square test to examine the association of comorbidities with mortality.

RESULTS Mortality was higher in CCI scores >4 (odds ratio [OR]: 8.83; 95% confidence interval [CI] = 1.81–43.01). The CCI score had moderate discrimination ability (AUC 76.1%; 95% CI = 0.661–0.881). Chronic kidney disease (CKD) (OR: 18.00, 95% CI = 2.19–147.51), congestive heart failure (CHF) (OR: 4.25, 95% CI = 1.23–14.75), and uncontrolled diabetes mellitus (DM) (OR: 18.429, 95% CI = 2.19–155.21) increased the risk of 28-day mortality.

CONCLUSIONS The CCI score could predict the 28-day mortality of critical COVID-19 patients. The coexistence of CKD, CHF, DM, peripheral vascular disease, and peptic ulcer in COVID-19 patients should be considered for patient management.
Light distribution technology of light-emitting-diode automotive lamps based on rippling pattern
Authors: Zhuang Jian
Published: June 2023

In this era of led-emitting-diode (LED) lighting, it is important to find a universal pattern structure for the LED automotive lamp optics. This paper starts with the secondary light distribution design method of a rippling pattern through the explanation and simulation analysis of its design principle and determines a 4′′round signal lamp scheme with good performance. It then completes the proofing verification of this scheme, and the test results achieve the original design goal. Additionally, several examples of other lamp types with the rippling pattern are introduced, including a 4′′ round halo lamp and 7′′ transit lamp.
Design of silica-PLC LP11 mode rotator based on adiabatic mode conversion
Authors: Sato Takanori , Fujisawa Takeshi , Wada Masaki , Mori Takayoshi , Sakamoto Taiji , Imada Ryota , Matsui Takashi , Nakajima Kazuhide , Saitoh Kunimasa
Published: June 2023

A silica-based LP11 mode rotator, which is one of the basic and indispensable optical components for space division multiplexing, with multiple tapered trenches is proposed. Compared with the conventional interference-based LP11 mode rotator with a simple L-shape waveguide, the proposed LP11 mode rotator has many advantages in a mode conversion efficiency, an insertion loss, and a fabrication tolerance because the operation principle is based on the adiabatic mode conversion. By using an approach of the shortcut to adiabaticity, the proposed device is effectively miniaturized rather than the standard tapered structures. Among the LP11 mode rotators in the silica-based mode multi/demultiplexers, the proposed type will be a considerably promising candidate.
Effect of urban heat island mitigation strategies on precipitation and temperature in Montreal, Canada: Case studies
Authors: Lauer Audrey , Pausata Francesco S. R. , Leroyer Sylvie , Argueso Daniel
Published: June 2023

High-resolution numerical weather prediction experiments using the Global Environmental Multiscale (GEM) model at a 250-m horizontal resolution are used to investigate the effect of the urban land-use on 2-m surface air temperature, thermal comfort, and rainfall over the Montreal (Canada) area. We focus on two different events of high temperatures lasting 2–3 days followed by intense rainfall: one is a large-scale synoptic system that crosses Montreal at night and the other is an afternoon squall line. Our model shows an overall good performance in adequately capturing the surface air temperature, dew-point temperature and rainfall during the events, although the precipitation pattern seems to be slightly blocked upwind of the city. Sensitivity experiments with different land use scenarios were conducted. Replacing all urban surfaces by low vegetation showed an increase of human comfort, lowering the heat index during the night between 2° and 6°C. Increasing the albedo of urban surfaces led to an improvement of comfort of up to 1°C during daytime, whereas adding street-level low vegetation had an improvement of comfort throughout the day of up to 0.5°C in the downtown area. With respect to precipitation, significant differences are only seen for the squall line event, for which removing the city modifies the precipitation pattern. For the large-scale synoptic system, the presence of the city does not seem to impact precipitation. These findings offer insight on the effects of urban morphology on the near-surface atmospheric conditions.
The chloride antiporter CLCN7 is a modifier of lysosome dysfunction in FIG4 and VAC14 mutants
Authors: Cao Xu , Lenk Guy M. , Mikusevic Vedrana , Mindell Joseph A. , Meisler Miriam H.
Published: June 2023

The phosphatase FIG4 and the scaffold protein VAC14 function in the biosynthesis of PI(3,5)P2, a signaling lipid that inhibits the lysosomal chloride transporter ClC-7. Loss-of-function mutations of FIG4 and VAC14 reduce PI(3,5)P2 and result in lysosomal disorders characterized by accumulation of enlarged lysosomes and neurodegeneration. Similarly, a gain of function mutation of CLCN7 encoding ClC-7 also results in enlarged lysosomes. We therefore tested the ability of reduced CLCN7 expression to compensate for loss of FIG4 or VAC14. Knock-out of CLCN7 corrected lysosomal swelling and partially corrected lysosomal hyperacidification in FIG4 null cell cultures. Knockout of the related transporter CLCN6 (ClC-6) in FIG4 null cells did not affect the lysosome phenotype. In the Fig4 null mouse, reduction of ClC-7 by expression of the dominant negative CLCN7 variant p.Gly215Arg improved growth and neurological function and increased lifespan by 20%. These observations demonstrate a role for the CLCN7 chloride transporter in pathogenesis of FIG4 and VAC14 disorders. Reduction of CLCN7 provides a new target for treatment of FIG4 and VAC14 deficiencies that lack specific therapies, such as Charcot-Marie-Tooth Type 4J and Yunis-Varón syndrome.
Community and facility-level barriers to achieving UHC in Kono District, Sierra Leone and Maryland County, Liberia
Authors: Higgins Julia , JEROME Jean Gregory , Boima Foday , Dally Emily , Krangar Luke , Boley Emma Jean , Toussaint Sterman , Dibba Yusupha , Kachimanga Chiyembekezo , Mhango Michael , Chung Vivian , Watson Samuel ,   one behalf of the Impact Consortium
Published: June 2023

Universal Health Coverage (UHC) is achieved when individuals and communities receive the health services they need without suffering financial hardship. However, many countries face barriers to building health systems that enable the availability of affordable, accessible care. The goal of this study was to present a model of local monitoring of barriers and to provide a roadmap for designing interventions that improve access to and use of healthcare delivery systems. We conducted household, individual, and health facility surveys in seven catchment areas in Sierra Leone and Liberia between December 2019 and March 2020. A two-stage cluster sampling method was used to sample households and individuals, and all health facilities were included. We divide access barriers into demand (patient-side care seeking behavior), supply (availability of facilities and services), and their intersection (affordability, spending, and use rates). Among the 2,576 respondents within our 1,051 surveyed households, the propensity to seek care when ill was reported at 90% in Sierra Leone (n = 1,283) and 70% in Liberia (n = 806). We estimated that 31% of households spent greater than 10% of their total expenditure on healthcare in a month, and that 14.5% of households spent greater than 25%. Overall, the general service readiness index mean score for all health centers was around 70%. The greatest hindrance to service readiness was the availability of essential medicines, with facilities reporting an average score of 32% in Sierra Leone and 63% in Liberia. Our evidence suggests that the cost of care is both a barrier to care-seeking and a persisting problem among care-seeking patients. Lack of service availability (essential equipment and medicines), poses a risk to high-quality care. The research team recommends deploying interventions (visit cost subsidies, supply chain improvements) targeted at resolving these issues in order to advance the goal of achieving UHC.
Genome-wide analysis of long noncoding RNAs and their association in regulating the metamorphosis of the Sarcophaga peregrina (Diptera: Sarcophagidae)
Authors: Shang Yanjie , Feng Yakai , Ren Lipin , Zhang Xiangyan , Yang Fengqin , Zhang Changquan , Guo Yadong
Published: June 2023

Background

The flesh fly, Sarcophaga peregrina (Diptera: Sarcophagidae), is an important hygiene pest, that causes myiasis in humans and other mammals, typically livestock, and as a vector for various parasitic agents, including bacteria, viruses, and parasites. The role of long non-coding RNAs (lncRNAs) in regulating gene expression during metamorphosis of the flesh fly has not been well established.

Methodology/Principal findings

In this study, we performed genome-wide identification and characterization of lncRNAs from the early pupal stage (1-days pupae), mid-term pupal stage (5-days pupae), and late pupal stage (9-days pupae) of S. peregrina by RNA-seq, and a total of 6921 lncRNAs transcripts were identified. RT-qPCR and enrichment analyses revealed the differentially expressed lncRNAs (DE lncRNAs) that might be associated with insect metamorphosis development. Furthermore, functional analysis revealed that the DE lncRNA (SP_lnc5000) could potentially be involved in regulating the metamorphosis of S. peregrina. RNA interference of SP_lnc5000 caused reduced expression of metamorphosis-related genes in 20-hydroxyecdysone (20E) signaling (Br-c, Ftz-F1), cuticle tanning pathway (TH, DOPA), and chitin related pathway (Cht5). Injection of dsSP_lnc5000 in 3rd instar larvae of S. peregrina resulted in deformed pupae, stagnation of pupal-adult metamorphosis, and a decrease in development time of pupal, pupariation rates and eclosion rates. Hematoxylin-eosin staining (H&E), scanning electron microscope (SEM) observation and cuticle hydrocarbons (CHCs) analysis indicated that SP_lnc5000 had crucial roles in the metamorphosis developmental by modulating pupal cuticular development.

Conclusions/Significance

We established that the lncRNA SP_lnc5000 potentially regulates the metamorphosis of S. peregrina by putatively affecting the structure and composition of the pupal cuticle. This study enhances our understanding of lncRNAs as regulators of metamorphosis in S. peregrina, and provide valuable insights into the identification of potential targets for vector control and the development of effective strategies for controlling the spread of myiasis and parasitic diseases.
A simplified function-first method for the discovery and optimization of bispecific immune engaging antibodies
Authors: Shepherd Alex , Bennychen Bigitha , Marcil Anne , Bloemberg Darin , Pon Robert A. , Weeratna Risini D. , McComb Scott
Published: June 2023

Bi-specific T-cell engager antibodies (BiTEs) are synthetic fusion molecules that combine multiple antibody-binding domains to induce active contact between T-cells and antigen expressing cells in the body. Blinatumomab, a CD19-CD3 BiTE is now a widely used therapy for relapsed B-cell malignancies, and similar BiTE therapeutics have shown promise for treating various other forms of cancer. The current process for new BiTE development is time consuming and costly, requiring characterization of the individual antigen binding domains, followed by bi-specific design, protein production, purification, and eventually functional screening. Here, we sought to establish a more cost-efficient approach for generating novel BiTE sequences and assessing bioactivity through a function first approach without purification. We generate a plasmid with a bi-modular structure to allow high-throughput exchange of either binding arm, enabling rapid screening of novel tumour-targeting single chain variable (scFv) domains in combination with the well-characterized OKT3 scFv CD3-targeting domain. We also demonstrate two systems for high throughput functional screening of BiTE proteins based on Jurkat T cells (referred to as BiTE-J). Using BiTE-J we evaluate four EGFRvIII-scFv sequenced in BiTE format, identifying two constructs with superior activity for redirecting T-cells against the EGFRvIII-tumour specific antigen. We also confirm activity in primary T cells, where novel EGFRvIII-BiTEs induced T cell activation and antigen selective tumor killing. We finally demonstrate similar exchange the CD3-interacting element of our bi-modular plasmid. By testing several novel CD3-targeting scFv elements for activity in EGFRvIII-targeted BiTEs, we were able to identify highly active BiTE molecules with desirable functional activity for downstream development. In summary, BiTE-J presents a low cost, high-throughput method for the rapid assessment of novel BiTE molecules without the need for purification and quantification.
Healthcare utilization for atopic dermatitis: An analysis of the 2010–2018 health insurance review and assessment service national patient sample data
Authors: Kim Sowon , Lee Ye-Seul , Yeo Jiyoon , Lee Donghyo , Ko Dong Kun , Ha In-Hyuk
Published: June 2023

This cross-sectional, retrospective, observational study aimed to analyze the distribution and healthcare usage patterns of patients with atopic dermatitis using the 2010–2018 Health Insurance Review and Assessment Service data. Patients diagnosed with atopic dermatitis in Korea between January 2010 and December 2018 and registered in the Health Insurance Review and Assessment national database were identified, and 270,008 patients who used healthcare services at least once during this period were evaluated to ascertain the healthcare usage patterns and treatment methods for atopic dermatitis. The number of patients with atopic dermatitis plateaued during the study period, while the number of claims and total expenses increased by a small margin. Atopic dermatitis prevalence was the highest among patients aged <5 years (31.4%), followed by those aged 5–14 years (23.53%) and 15–24 years (15.33%). However, the prevalence in these age groups showed a decreasing trend over time. The most used Western medicine treatments were injections and oral medications involving topical corticosteroids, antihistamine agents, and oral steroids, while it was acupuncture therapy in Korean medicine. The frequency of the most frequently prescribed medication, topical corticosteroid, showed a decreasing trend over time. The findings in this study will inform healthcare policy makers and clinicians across different countries on the usage trends of Western medicine and Korean medicine treatment.
Identification of potential biomarkers in active Lyme borreliosis
Authors: Tjernberg Ivar , Lager Malin , Furset Jensen Guro , Eikeland Randi , Nyman Dag , Brudin Lars , Henningsson Anna J.
Published: June 2023

Objectives

Lyme serology does not readily discriminate an active Lyme borreliosis (LB) from a previous Borrelia infection or exposure. Here, we aimed to investigate a large number of immunological protein biomarkers to search for an immunological pattern typical for active LB, in contrast to patterns found in healthy blood donors, a proportion of whom were previously exposed to Borrelia.

Methods

Serum samples from well-characterised adult patients with ongoing LB and healthy blood donors were included and investigated using a proximity extension assay (provided by Olink®) by which 92 different immune response-related human protein biomarkers were analysed simultaneously.

Results

In total, 52 LB patients and 75 healthy blood donors were included. The blood donors represented both previously Borrelia exposed (n = 34) and not exposed (n = 41) based on anti-Borrelia antibody status. Ten of the examined 92 proteins differed between patients and blood donors and were chosen for further logistic regression (p<0.1). Six proteins were statistically significantly different between LB patients and blood donors (p<0.05). These six proteins were then combined in an index and analysed using receiver-operating-characteristic curve analysis showing an area under the curve of 0.964 (p<0.001).

Conclusions

The results from this study suggest that there is an immunological protein pattern that can distinguish a present Borrelia infection from a previous exposure as well as anti-Borrelia antibody negative blood donors. Although this method is not adapted for routine clinical use at this point, the possibility is interesting and may open new diagnostic opportunities improving the laboratory diagnostics of LB.
Gramsci e o jornalismo
Authors: Carvalheiro José
Published: June 2023

O pensamento de Gramsci sobre jornalismo, amplo e fragmentário, tem ocupado um lugar menor nas leituras da sua obra, ao contrário do que merece. Neste artigo juntamos todas as suas reflexões teóricas sobre o tema, que estão dispersas desde textos de imprensa dos anos 1910 até a um dos últimos Cadernos do Cárcere, escrito em 1934, precisamente sobre jornalismo. Estreitamente ligada ao projeto moderno de instrução e emancipação humana, a visão de Gramsci acerca da imprensa é indissociável da sua ação política, mas transcende-a, com uma vertente de análise sociológica que foi pioneira no campo marxista e olhou historicamente a incorporação do público popular num contexto mercantil.
Do homem a praça, da mulher a casa
Authors: Da Conceição Silva Manuela Sofia
Published: June 2023

A uma distância de cinquenta anos, as Novas Cartas Portuguesas, emblemáticas das lutas de mulheres pela igualdade entre géneros, em Portugal e no mundo, continuam a falar às gerações atuais, de modo algo semelhante ao que fizeram às mulheres da época. Associado ao feminismo quando as feministas se reconheceram nas histórias daquelas mulheres, as Novas Cartas questionam as relações de poder construídas pela sociedade, onde as desigualdades sociais, políticas e culturais, baseadas nas diferenças entre géneros, dita(va)m a «inferioridade» das mulheres em relação aos homens. Pretende-se, com este artigo, explorar em que medida o discurso literário das «Três Marias» foi fundamental para a crítica social da situação da mulher em Portugal, em particular no que se refere à situação profissional, à igualdade salarial, ao acesso ao emprego e ao direito à carreira, constituindo-se como uma referência para as transformações sociais, culturais e legislativas ocorridas depois da Revolução dos Cravos.
SUMRAY: R and Python Codes for Calculating Cancer Risk Due to Radiation Exposure of a Population
Authors: Sasaki Michiya , Furukawa Kyoji , Satoh Daiki , Shimada Kazumasa , Kudo Shin’ichi , Takagi Shunji , Takahara Shogo , Kai Michiaki
Published: June 2023

Background: Quantitative risk assessments should be accompanied by uncertainty analyses of the risk models employed in the calculations. In this study, we aim to develop a computational code named SUMRAY for use in cancer risk projections from radiation exposure taking into account uncertainties. We also aim to make SUMRAY publicly available as a resource for further improvement of risk projection.Materials and Methods: SUMRAY has two versions of code written in R and Python. The risk models used in SUMRAY for all-solid-cancer mortality and incidence were those published in the Life Span Study of a cohort of the atomic bomb survivors in Hiroshima and Nagasaki. The confidence intervals associated with the evaluated risks were derived by propagating the statistical uncertainties in the risk model parameter estimates by the Monte Carlo method.Results and Discussion: SUMRAY was used to calculate the lifetime or time-integrated attributable risks of cancer under an exposure scenario (baseline rates, dose[s], age[s] at exposure, age at the end of follow-up, sex) specified by the user. The results were compared with those calculated using another well-known web-based tool, Radiation Risk Assessment Tool (Rad- RAT; National Institutes of Health), and showed a reasonable agreement within the estimated confidential interval. Compared with RadRAT, SUMRAY can be used for a wide range of applications, as it allows the risk projection with arbitrarily specified risk models and/or population reference data.Conclusion: The reliabilities of SUMRAY with the present risk-model parameters and their variance-covariance matrices were verified by comparing them with those of the other codes. The SUMRAY code is distributed to the public as an open-source code under the Massachusetts Institute of Technology license.
The Effect of Pumpkin Fruit Ripeness (Cucurbita moschata. D) on Total Flavonoid Levels and Antioxidant Activity
Authors: Astutik Linda , Yanti Eka Fitri
Published: June 2023

Pumpkin fruit (Cucurbita moschata. D) belongs the Cucurbitaceae family which is a functional vegetable widely distributed in Indonesia because it has nutritional value and health benefits. This study aims to determine the value of total flavonoid content and antioxidant activity in the ethanol extract of unripe, mature and ripened pumpkin. Simplicia powder was extracted by maceration method using 96% solvent. Testing the total flavonoid content with the addition of AlCl3 at a wavelength of 425 nm and the antioxidant activity test was carried out using DPPH (1-1-diphenyl-2-picrylhydrazyl) as a free radical with a maximum wavelength of 515 nm using microplate reader. The results of the study concluded that the ethanolic extract of pumpkin flesh had a total flavonoid content of 0,146 mgQE/100g in unripe fruit, 0,221 mgQE/100g in mature pumpkin, 0,191 mgQE/100g in ripened pumpkin. The antioxidant activity of the ethanol extract of unripe, mature, and ripened pumpkin fruit obtained was not active or did not completely reduce free radicals.
Bioactive Compound in Solanum torvum and Its Potential as Functional Food and Drink: A Review
Authors: Putri Nina Nurazizah Purnomo , Anggriani Rista , Sukardi Sukardi
Published: June 2023

Solanum torvum is a fruit used as food and has medical properties. This study aims to provide an updated understanding of Solanum Torvum’s health benefits as a functional food through a study literature review. The research utilizes electronic databases (PubMed, Science Direct, Scopus, Nature, Clinical Key, and Springer) from July-October 2022. Solanum Torvum contains various bioactive components, vitamins, mineral, nutritions. It shows that Solanum torvum has potential sources as functional food. However, studies about solanum torvum consumption as a functional food and the clinical trial of its health benefits in humans are still limited. Further in vivo and in vitro studies are necessary to present the effect of solanum torvum consumption on health.
Chemical Profile and in-silico Docking Studies on Bioactives from Essential Oil of Cymbopogan pendulus Targeting Penicillin Binding Proteins (PBPs) in Bacteria
Authors: Sharma Arun Dev , Kaur Inderjeet
Published: June 2023

Antibiotic resistance in bacteria is the major concern worldwide. PBP (Penicillin binding proteins) have been cited as an appropriate target for therapeutic drug design. In the present study molecular docking followed by wet lab validation was designed to estimate the effect of potent bioactive molecules from Cymbopogan pendulus essential oil against PBP5 protein. GC-FID (gas chromatography with flame-ionization detection) based composition profile, and in-silico docking study was conducted by using CB-dock 2 analysis followed by 2D and 3D interactions. GC-FID revealed Limonene, Neral, Geranial, Linalool, Myrcene as major and minor compounds in Cymbopogan pendulus essential oil. The docking score indicated effective binding of ligands to PBP5. Interactions results indicated that, PBP5/ligand complexes form hydrogen and hydrophobic interactions. Wet lab study validated the anti-bacterial potential of oil against gram-positive and gram-negative bacteria. Therefore, essential oil from Cymbopogan pendulus essential oil may represent potential herbal treatment to mitigate bacterial infections.
Apakah Overprotektif Orang Tua Berkorelasi Dengan Agresivitas? Studi Pada Mahasiswa Rantau Asal Daerah Yang Pernah Berkonflik Sosial
Authors: Wattimena Marice Meigy , Huwae Arthur
Published: June 2023

Aggressive behavior is a form of action whose purpose is to hurt or injure another person, physically or verbally. There are several factors behind the emergence of aggressive behavior, one of which is overprotective parenting. Overprotective parenting causes children to feel pressured and less free. Moving on from this, this study aims to find out the correlation between overprotective parents and the aggressiveness of wander students from regions who have had social conflicts. The research method used is correlational quantitative. The study participants were 60 students who came from areas that had experienced social conflict (Moluccas, Papua, Central Borneo and Central Sulawesi) using the incidental sampling technique. Data collection used the Parent Overprotectiveness Scale (α = 0.863) and the Aggressiveness Scale (α = 0.898). The research results prove that the research hypothesis is accepted (r = 0.387 and sig. = 0.002). It can be concluded that the increased aggressiveness of wander students from regions who have had social conflicts is due to an increase in parental overprotectiveness. Perilaku agresif merupakan bentuk tindakan yang tujuannya meyakiti atau melukai orang lain, secara fisik maupun verbal. Terdapat beberapa faktor yang melatarbelakangi timbulnya perilaku agresif, salah satunya pola pengasuhan orang tua yang overprotektif. Pengasuhan orang tua yang overprotektif menyebabkan anak merasa tertekan dan kurang bebas. Beranjak dari hal tersebut, maka penelitian ini bertujuan mencari tahu korelasi antara overprotektif orang tua dan agresivitas mahaswa rantau asal daerah yang pernah berkonflik sosial. Metode penelitian yang digunakan yaitu kuantitatif korelasional. Partisipan penelitian sebanyak 60 mahasiswa yang berasal dari daerah yang pernah berkonflik sosial (Maluku, Papua, Kalimantan Tengah, dan Sulawesi Tengah) dengan teknik insidental sampling. Pengumpulan data menggunakan Skala Overprotektif Orang Tua (α = 0,863) dan Skala Agresivitas (α = 0,898). Hasil penelitian membuktikan bahwa hipotesis penelitian diterima (r = 0,387 dan sig. = 0,002). Dapat disimpulkan bahwa meningkatnya agresivitas mahasiswa rantau asal daerah yang pernah berkonflik sosial, karena adanya peningkatan overprotektif orang tua yang diterapkan.
"Together We Are Stronger": Pencapaian Kepuasan Perkawinan Pasangan dengan Anak Penyandang Autism Spectrum Disorder (ASD)
Authors: Muqoddam Faqihul , Yoenanto Nono Hery , Suminar Dewi Retno
Published: June 2023

Couples caring for children with Autism Spectrum Disorder (ASD) are always associated with lower marital satisfaction and higher divorce potential. But not all of them are like that, because they can also maintain and be able to achieve marital satisfaction while caring for children with ASD. The study aims to explore the experience of achieving marital satisfaction in couples caring for children with ASD. Qualitative research methods with a phenomenological approach are used in this study. Data was collected using semi-structured interviews, while data were analyzed through Interpretative Phenomenological Analysis (IPA). The participants involved consisted of 7 marriages couples and 4 informants. The Couple Satisfaction Index (CSI-16) questionnaire was used as a preliminary study to identify couples who have high marital satisfaction. Validity test using member check technique and triangulation of data sources. The results of the study resulted in 5 main themes, such as the first experience of couples while caring for a child with ASD; fulfillment of couples needs while caring for a child with ASD; fulfillment of couple's expectations; actions taken to achieve marital satisfaction; and duration of marital satisfaction achievement. The process of achieving marital satisfaction in couples caring for children with ASD varies according to duration, but always begins with a downturn and continues with the desire to achieve marital satisfaction.Pasangan dengan anak penyandang Autism Spectrum Disorder (ASD) selalu dikaitkan dengan kepuasan perkawinan yang rendah dan potensi perceraian yang lebih tinggi. Namun tidak semuanya demikian, karena mereka juga bisa mempertahankan dan mampu mencapai kepuasan perkawinan selama mengasuh anak penyandang ASD. Tujuan penelitian untuk mengeksplorasi pengalaman pencapaian kepuasan perkawinan pada pasangan dengan anak penyandang ASD. Metode penelitian kualitatif dengan pendekatan fenomenologi digunakan dalam penelitian ini. Pengumpulan data menggunakan wawancara semi-terstruktur, sedangkan data dianalisis melalui Interpretative Phenomenological Analysis (IPA). Partisipan yang terlibat terdiri dari 7 pasangan dan 4 informan. Kuesioner Couple Satisfaction Index (CSI-16) digunakan sebagai preliminary study untuk mengidentifikasi pasangan yang memiliki kepuasan perkawinan yang tinggi. Uji validitas menggunakan teknik member check dan triangulasi sumber data. Hasil penelitian menghasilkan 5 tema utama, seperti pengalaman awal pasangan selama mengasuh anak penyandang ASD; pemenuhan kebutuhan pasangan selama mengasuh anak penyandang ASD; pemenuhan harapan pasangan; tindakan yang dilakukan dalam mencapai kepuasan perkawinan; dan durasi pencapaian kepuasan perkawinan. Proses pencapaian kepuasan perkawinan pada pasangan yang mengasuh anak penyandang ASD cenderung berbeda sesuai durasi, namun selalu diawali dengan keterpurukan dan berlanjut pada keinginan untuk mencapai kepuasan perkawinan.
Implications of Electronic Word-of-Mouth on Hotel Profitability: An Investigation beyond Room Revenue
Authors:   Department of Health & Human Performance, Middle Tennessee State University, U.S.A, Tan Karen Pei-Sze , Zou Suiwen Sharon ,   Department of Recreation, Sport & Tourism, University of Illinois UrbanaChampaign, U.S.A., Li Xiang Robert ,   Department of Tourism & Hospitality Management, Temple University, U.S.A.
Published: June 2023

Interest has been growing in the financial impact of online reviews, an important form of electronic word-of-mouth (eWOM), on hotel performance. Existing research in this area is overwhelmingly focused on the relationship between online reviews and room-related revenue. For a more comprehensive understanding of online reviews’ financial impacts, this study investigates the value of these reviews relative to hotels’ non-room revenue, total revenue and gross operating profit (GOP). To the authors’ best knowledge, this study is the first to link hotels’ online reviews or any form of eWOM to GOP, a firm-level outcome. A panel dataset comprising the annual operating performance of 104 hotels in Asia was assembled and matched with 50,732 TripAdvisor reviews. Analyses indicated that review valence, variance and volume correlated to varying degrees with hotels’ GOP but demonstrated limited association with non-room revenue. Our findings also indicated that online reviews more strongly influenced room revenue and total revenue than non-room revenue and GOP. Overall, the positive effects associated with room revenue demonstrated selective generalizability to GOP. This in turn reflects the insufficiency of relying solely on room revenue indicators (i.e. average daily rate, occupancy and revenue per available room) to fully understand the impact of online reviews.
Demand response based microgrid's economic dispatch
Authors: Saeed Muhammad Hammad , Rana MD Sohel , Kausaraahmed MD , El-Bayeh Claude Ziad , Wang Fangzong
Published: June 2023

The development of energy management tools for next-generation Distributed Energy Resources (DER) based power plants, such as photovoltaic, energy storage units, and wind, helps power systems be more flexible. Microgrids are entities that coordinate DERs in a persistently more decentralized fashion, hence decreasing the operational burden on the main grid and permitting them to give their full benefits. A new power framework has emerged due to the integration of DERs-based microgrids into the conventional power system. With the rapid advancement of microgrid technology, more emphasis has been placed on maintaining the microgrids' long-term economic feasibility while ensuring security and stability. The objective of this research is to provide a multi-objective economic operation technique for microgrids containing air-conditioning clusters (ACC) taking demand response into account. A dynamic price mechanism is proposed, accurately reflecting the system's actual operational status. For economic dispatch, flexible loads and air conditioners are considered demand response resources. Then, a consumer-profit model and an AC operating cost model are developed, with a set of pragmatic constraints of consumer comfort. The generation model is then designed to reduce the generation cost. Finally, a microgrid simulation platform is developed in MATLAB/Simulink, and a case is designed to evaluate the proposed method's performance. The findings show that consumer profit increases by 69.2% while ACC operational costs decrease by 18.2%. Moreover, generation costs are reduced without sacrificing customer satisfaction.
Human Action Recognition (HAR) Classification Using MediaPipe and Long Short-Term Memory (LSTM)
Authors: Putra Ichsan Arsyi , Nurhayati Oky Dwi , Eridani Dania
Published: June 2023

Human Action Recognition is an important research topic in Machine Learning and Computer Vision domains. One of the proposed methods is a combination of MediaPipe library and Long Short-Term Memory concerning the testing accuracy and training duration as indicators to evaluate the model performance. This research tried to adapt proposed LSTM models to implement HAR with image features extracted by MediaPipe library. There would be a comparison between LSTM models based on their testing accuracy and training duration. This research was conducted under OSEMN methods (Obtain, Scrub, Explore, Model, and iNterpret). The dataset was preprocessed Weizmann dataset with data preprocessing and data augmentation implementations. Video features extracted by MediaPipe: Pose was used in training and validation processes on neural network models focusing on Long Short-Term Memory layers. The processes were finished by model performance evaluation based on confusion matrices interpretation and calculations of accuracy, error rate, precision, recall, and F1score. This research yielded seven LSTM model variants with the highest testing accuracy at 82% taking 10 minutes and 50 seconds of training duration.
In vitro Evaluation of Sorghum Genotypes for their Resistance to Aflatoxin B1 Contamination
Authors: Taye Wondimeneh
Published: June 2023

Sorghum is Ethiopia's main staple meal and the most significant nutritional security crop. Aflatoxin contamination in sorghum grains has been documented all across the world, including Ethiopia. Therefore, the use of resistant genotype is considered to be the most feasible means to mitigate the problem. Thus, 20 sorghum genotypes were evaluated in vitro for their reaction to aflatoxin contamination at Haramaya University School of Plant Sciences, crop protection laboratory. Mycotoxin analysis was done using enzyme-linked immunosorbent assay (ELISA). The findings showed that the sorghum genotypes tested for aflatoxin B1 responses had considerably varied responses. Long Muyera was identified as the most sensitive genotype to aflatoxin B1 contamination (34.1 g kg-1); with contamination levels significantly beyond the maximum tolerated limit (10 g kg-1). From the highland genotypes with a contamination level of 6.9 g kg-1, genotype W-5 (Weger-5) was relatively resistant to aflatoxin B1. Teshale genotype was the least resistant of the lowland sorghum genotypes examined. Except for genotype Long Muyera, highland sorghum genotypes were less sensitive to aflatoxin B1 contamination than lowland sorghum genotypes. As a result, it is worthwhile to choose and use genotypes that remain resistant in certain areas.