bluuebunny/crossref_metadata_2025 huggingface dataset:
Mosquitoes, Infectious Diseases, and Cancer: A Connection to Study?
Authors: Brisola Marcondes Carlos , Benelli Giovanni
Published: December 2019

Mosquitoes (Diptera: Culicidae) are vectors of pathogens and parasites of great medical and veterinary relevance. The possible association between mosquitoes, infectious diseases, and cancer has been investigated. Despite its potential importance, there is a severe lack of research data on the topic. Herein, current knowledge, tenuous links, and related challenges on the topic were examined, grouping information under four major hypotheses. The first hypothesis is that the infection of mosquito-vectored parasites, with special reference to Plasmodium spp., may lead to cancer. The International Agency for Research on Cancer stated that being infected by Plasmodium falciparum malaria in holoendemic areas is probably carcinogenic to humans (group 2A), considering that P. falciparum infection is able to reactivate the Epstein–Barr virus, leading to endemic Burkitt lymphoma. Also, malaria was recently associated with a cancer incidence increase in the United States. The second hypothesis is that cancer may be spread directly through mosquito bites: Aedes mosquitoes transfer viable tumor cells among vertebrate hosts, even if no plausible mechanisms for these cells to develop cancer into the new host are known. As the third hypothesis, mosquito bites may lead to hypersensitivity, resulting in cancer. Hypersensitivity stimulated by mosquito bites links allergy, oncogenesis, and the Epstein–Barr virus, causing Burkitt lymphoma. One may argue that pathogens transmitted by mosquitoes, such as viruses, may be carcinogenic. However, no detailed research evidences are available to substantiate this last hypothesis. However, despite the intriguing hypotheses outlined above, there is a severe lack of data showing cancer development in organisms exposed to mosquitoes transmitting parasites or pathogens. According to One Health criteria, this benchmark is aimed to outline major questions on this public health issue, stressing the need of multidisciplinary research and discussion.
The Interplay between the Endocannabinoid System, Epilepsy and Cannabinoids
Authors: Kwan Cheung Keith A. , Peiris Hassendrini , Wallace Geoffrey , Holland Olivia J. , Mitchell Murray D.
Published: December 2019

Epilepsy is a neurological disorder that affects approximately 50 million people worldwide. There is currently no definitive epilepsy cure. However, in recent years, medicinal cannabis has been successfully trialed as an effective treatment for managing epileptic symptoms, but whose mechanisms of action are largely unknown. Lately, there has been a focus on neuroinflammation as an important factor in the pathology of many epileptic disorders. In this literature review, we consider the links that have been identified between epilepsy, neuroinflammation, the endocannabinoid system (ECS), and how cannabinoids may be potent alternatives to more conventional pharmacological therapies. We review the research that demonstrates how the ECS can contribute to neuroinflammation, and could therefore be modulated by cannabinoids to potentially reduce the incidence and severity of seizures. In particular, the cannabinoid cannabidiol has been reported to have anti-convulsant and anti-inflammatory properties, and it shows promise for epilepsy treatment. There are a multitude of signaling pathways that involve endocannabinoids, eicosanoids, and associated receptors by which cannabinoids could potentially exert their therapeutic effects. Further research is needed to better characterize these pathways, and consequently improve the application and regulation of medicinal cannabis.
MicroRNA-451 and Genistein Ameliorate Nonalcoholic Steatohepatitis in Mice
Authors: Gan Mailin , Shen Linyuan , Fan Yuan , Tan Ya , Zheng Ting , Tang Guoqing , Niu Lili , Zhao Ye , Chen Lei , Jiang Dongmei , Li Xuewei , Zhang Shunhua , Zhu Li
Published: December 2019

Effective, targeted therapy for chronic liver disease nonalcoholic steatohepatitis (NASH) is imminent. MicroRNAs (miRNAs) are a potential therapeutic target, and natural products that regulate miRNA expression may be a safe and effective treatment strategy for liver disease. Here, we investigated the functional role of miR-451 and the therapeutic effects of genistein in the NASH mouse model. MiR-451 was downregulated in various types of liver inflammation, and subsequent experiments showed that miR-451 regulates liver inflammation via IL1β. Genistein is a phytoestrogen with anti-inflammatory and anti-oxidant effects. Interestingly, we found that the anti-inflammatory effects of genistein were related to miR-451 and was partially antagonized by the miR-451 inhibitor. MiR-451 overexpression or genistein treatment inhibited IL1β expression and inflammation. Taken together, this study shows that miR-451 has a protective effect on hepatic inflammation, and genistein can be used as a natural promoter of miR-451 to ameliorate NASH.
Ginsenosides Rb1 and Rg1 Protect Primary Cultured Astrocytes against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury via Improving Mitochondrial Function
Authors: Xu Meng , Ma Qing , Fan Chunlan , Chen Xue , Zhang Huiming , Tang Minke
Published: December 2019

This study aimed to evaluate whether ginsenosides Rb1 (20-S-protopanaxadiol aglycon) and Rg1 (20-S-protopanaxatriol aglycon) have mitochondrial protective effects against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury in primary mouse astrocytes and to explore the mechanisms involved. The OGD/R model was used to mimic the pathological process of cerebral ischemia-reperfusion in vitro. Astrocytes were treated with normal conditions, OGD/R, OGD/R plus Rb1, or OGD/R plus Rg1. Cell viability was measured to evaluate the cytotoxicity of Rb1 and Rg1. Intracellular reactive oxygen species (ROS) and catalase (CAT) were detected to evaluate oxidative stress. The mitochondrial DNA (mtDNA) copy number and mitochondrial membrane potential (MMP) were measured to evaluate mitochondrial function. The activities of the mitochondrial respiratory chain (MRC) complexes I–V and the level of cellular adenosine triphosphate (ATP) were measured to evaluate oxidative phosphorylation (OXPHOS) levels. Cell viability was significantly decreased in the OGD/R group compared to the control group. Rb1 or Rg1 administration significantly increased cell viability. Moreover, OGD/R caused a significant increase in ROS formation and, subsequently, it decreased the activity of CAT and the mtDNA copy number. At the same time, treatment with OGD/R depolarized the MMP in the astrocytes. Rb1 or Rg1 administration reduced ROS production, increased CAT activity, elevated the mtDNA content, and attenuated the MMP depolarization. In addition, Rb1 or Rg1 administration increased the activities of complexes I, II, III, and V and elevated the level of ATP, compared to those in the OGD/R groups. Rb1 and Rg1 have different chemical structures, but exert similar protective effects against astrocyte damage induced by OGD/R. The mechanism may be related to improved efficiency of mitochondrial oxidative phosphorylation and the reduction in ROS production in cultured astrocytes.
Short and Long Term Clinical and Immunologic Follow up after Bone Marrow Mesenchymal Stromal Cell Therapy in Progressive Multiple Sclerosis—A Phase I Study
Authors: Iacobaeus Ellen , Kadri Nadir , Lefsihane Katia , Boberg Erik , Gavin Caroline , Törnqvist Andrén Anton , Lilja Anders , Brundin Lou , Blanc Katarina Le
Published: December 2019

Bone marrow derived mesenchymal stromal cells (BM-MSCs) have emerged as a possible new therapy for Multiple Sclerosis (MS), however studies regarding efficacy and in vivo immune response have been limited and inconclusive. We conducted a phase I clinical study assessing safety and clinical and peripheral immune responses after MSC therapy in MS. Seven patients with progressive MS were intravenously infused with a single dose of autologous MSC (1–2 × 106 MSCs/kg body weight). The infusions were safe and well tolerated when given during clinical remission. Five out of seven patients completed the follow up of 48 weeks post-infusion. Brain magnetic resonance imaging (MRI) showed the absence of new T2 lesions at 12 weeks in 5/6 patients, while 3/5 had accumulated new T2 lesions at 48 weeks. Patient expanded disability status scales (EDSS) were stable in 6/6 at 12 weeks but declined in 3/5 patients at 48 weeks. Early changes of circulating microRNA levels (2 h) and increased proportion of FOXP3+ Tregs were detected at 7 days post-infusion compared to baseline levels. In conclusion, MSC therapy was safe and well tolerated and is associated with possible transient beneficial clinical and peripheral immunotolerogenic effects.
Computer-Aided Planning in Orthognathic Surgery: A Comparative Study with the Establishment of Burstone Analysis-Derived 3D Norms
Authors: Ho Cheng-Ting , Denadai Rafael , Lai Hsin-Chih , Lo Lun-Jou , Lin Hsiu-Hsia
Published: December 2019

Three-dimensional (3D) computer-aided simulation has revolutionized orthognathic surgery treatment, but scarce 3D cephalometric norms have been defined to date. The purposes of this study were to (1) establish a normative database of 3D Burstone cephalometric measurements for adult male and female Chinese in Taiwan, (2) compare this 3D norm dataset with the two-dimensional (2D) Burstone norms from Caucasian and Singaporean Chinese populations, and (3) apply these 3D norms to assess the outcome of a computer-aided simulation of orthognathic surgery. Three-dimensional Burstone cephalometric analysis was performed on 3D digital craniofacial image models generated from cone-beam computed tomography datasets of 60 adult Taiwanese Chinese individuals with normal occlusion and balanced facial profile. Three-dimensional Burstone analysis was performed on 3D image datasets from patients with skeletal Class III pattern (n = 30) with prior computer-aided simulation. Three-dimensional Burstone cephalometric measurements showed that Taiwanese Chinese males had significantly (p < 0.05) larger anterior and posterior facial heights, maxillary length, and mandibular ramus height than females, with no significant (p > 0.05) difference for facial soft-tissue parameters. The 3D norm dataset revealed Taiwanese Chinese-specific facial characteristics, with Taiwanese presenting (p < 0.05) a more convex profile, protrusive maxillary apical bases, protruding mandible, protruding upper and lower lips, and a shorter maxillary length and lower facial height than Caucasians. Taiwanese had significantly (p < 0.05) larger maxillary projection, vertical height ratio, lower face throat angle, nasolabial angle, and upper lip protrusion than Singaporean Chinese. No significant (p > 0.05) difference was observed between 3D norms and computer-aided simulation-derived 3D patient images for horizontal skeletal, vertical skeletal, and dental measurements, with the exception of two dental parameters (p < 0.05). This study contributes to literature by providing gender- and ethnic-specific 3D Burstone cephalometric norms, which can assist in the multidisciplinary-based delivery of orthodontic surgical care for Taiwanese Chinese individuals worldwide, including orthodontic management, computer-assisted simulation, and outcome assessment.
13-gene DNA Methylation Analysis from Oral Brushing: A Promising Non Invasive Tool in the Follow-up of Oral Cancer Patients
Authors: Gissi Davide B. , Tarsitano Achille , Gabusi Andrea , Rossi Roberto , Attardo Giuseppe , Lenzi Jacopo , Marchetti Claudio , Montebugnoli Lucio , Foschini Maria P. , Morandi Luca
Published: December 2019

Background: This study aimed to evaluate the prognostic value of a non-invasive sampling procedure based on 13-gene DNA methylation analysis in the follow-up of patients previously treated for oral squamous cell carcinoma (OSCC). Methods: The study population included 49 consecutive patients treated for OSCC. Oral brushing sample collection was performed at two different times: before any cancer treatment in the tumor mass and during patient follow-up almost 6 months after OSCC treatment, within the regenerative area after OSCC resection. Each sample was considered positive or negative in relation to a predefined cut-off value. Results: Before any cancer treatment, 47/49 specimens exceeded the score and were considered as positive. Six months after OSCC resection, 16/49 specimens also had positive scores in the samples collected from the regenerative area. During the follow-up period, 7/49 patients developed locoregional relapse: 6/7 patients had a positive score in the regenerative area after OSCC resection. The presence of a positive score after oral cancer treatment was the most powerful variable related to the appearance of locoregional relapse. Conclusion: 13-gene DNA methylation analysis by oral brushing may have a clinical application as a prognostic non-invasive tool in the follow-up of patients surgically treated for OSCC.
The Innate Immune Cell Profile of the Cornea Predicts the Onset of Ocular Surface Inflammatory Disorders
Authors: Palomar Amaya Pérez del , Montolío Alberto , Cegoñino José , Dhanda Sandeep Kumar , Lio Chit Tong , Bose Tanima
Published: December 2019

Ocular surface inflammatory disorder (OSID) is a spectrum of disorders that have features of several etiologies whilst displaying similar phenotypic signs of ocular inflammation. They are complicated disorders with underlying mechanisms related to several autoimmune disorders, such as rheumatoid arthritis (RA), Sjögren’s syndrome, and systemic lupus erythematosus (SLE). Current literature shows the involvement of both innate and adaptive arms of the immune system in ocular surface inflammation. The ocular surface contains distinct components of the immune system in the conjunctiva and the cornea. The normal conjunctiva epithelium and sub-epithelial stroma contains resident immune cells, such as T cells, B cells (adaptive), dendritic cells, and macrophages (innate). The relative sterile environment of the cornea is achieved by the tolerogenic properties of dendritic cells in the conjunctiva, the presence of regulatory lymphocytes, and the existence of soluble immunosuppressive factors, such as the transforming growth factor (TGF)-β and macrophage migration inhibitory factors. With the presence of both innate and adaptive immune system components, it is intriguing to investigate the most important leukocyte population in the ocular surface, which is involved in immune surveillance. Our meta-analysis investigates into this with a focus on both infectious (contact lens wear, corneal graft rejection, Cytomegalovirus, keratitis, scleritis, ocular surgery) and non-infectious (dry eye disease, glaucoma, graft-vs-host disease, Sjögren’s syndrome) situations. We have found the predominance of dendritic cells in ocular surface diseases, along with the Th-related cytokines. Our goal is to improve the knowledge of immune cells in OSID and to open new dimensions in the field. The purpose of this study is not to limit ourselves in the ocular system, but to investigate the importance of dendritic cells in the disorders of other mucosal organs (e.g., lungs, gut, uterus). Holistically, we want to investigate if this is a common trend in the initiation of any disease related to the mucosal organs and find a unified therapeutic approach. In addition, we want to show the power of computational approaches to foster a collaboration between computational and biological science.
Evaluation of the Sex-and-Age-Specific Effects of PM2.5 on Hospital Readmission in the Presence of the Competing Risk of Mortality in the Medicare Population of Utah 1999–2009
Authors: Leiser Claire L , Smith Ken R , VanDerslice James A , Glotzbach Jason P , Farrell Timothy W , Hanson Heidi A
Published: December 2019

Acute ambient air pollution exposure increases risk of cardiac events. We evaluated sex-and-age-specific effects of PM2.5 on hospital readmission and death among 19,602 Medicare beneficiaries (Nevents = 30,510) who survived cardiovascular events including myocardial infarction (MI), heart failure (HF), ischemic heart disease (IHD), and cardiac arrhythmias in Utah from 1999–2009. Fine and Gray regression jointly modeled the effect of PM2.5 on readmission hazard rates while allowing for the competing risk of death. Models were stratified by age and sex and adjusted for Charlson Comorbidity Index, dual enrollment in Medicare Parts A and B, temperature, and household income. There were 2032 cardiac readmissions and 1420 deaths after discharge. Among males age 65–74 years admitted for HF, a 10 μm/m3 increase in PM2.5 was associated with a 30% increase in risk of readmission (p = 0.01). Among females age 75–84 admitted for HF, PM2.5 was associated with a 22% increase in risk of readmission (p = 0.01). Among females age 75–84 years admitted for IHD, PM2.5 was associated with a 25% decrease in readmission (p = 0.01), however this was explained by a 36% increase in risk of death (p = 0.01). Exposure to PM2.5 was associated with increased risk of readmission and death. Associations were dependent upon age, sex, and index condition.
Health in Preconception, Pregnancy and Postpartum Global Alliance: International Network Preconception Research Priorities for the Prevention of Maternal Obesity and Related Pregnancy and Long-Term Complications
Authors: Hill Briony , Skouteris Helen , Teede Helena J , Bailey Cate , Baxter Jo-Anna B , Bergmeier Heidi J , Borges Ana Luiza Vilela , Harrison Cheryce L , Jack Brian , Jorgensen Laura , Lim Siew , Montanaro Cynthia , Redman Leanne , Steegers Eric , Stephenson Judith , Sundseth Hildrun , Thangaratinam Shakila , Walker Ruth , Boyle Jacqueline A
Published: December 2019

The preconception period is a key public health and clinical opportunity for obesity prevention. This paper describes the development of international preconception priorities to guide research and translation activities for maternal obesity prevention and improve clinical pregnancy outcomes. Stakeholders of international standing in preconception and pregnancy health formed the multidisciplinary Health in Preconception, Pregnancy, and Postpartum (HiPPP) Global Alliance. The Alliance undertook a priority setting process including three rounds of priority ranking and facilitated group discussion using Modified Delphi and Nominal Group Techniques to determine key research areas. Initial priority areas were based on a systematic review of international and national clinical practice guidelines, World Health Organization recommendations on preconception and pregnancy care, and consumer and expert input from HiPPP members. Five preconception research priorities and four overarching principles were identified. The priorities were: healthy diet and nutrition; weight management; physical activity; planned pregnancy; and physical, mental and psychosocial health. The principles were: operating in the context of broader preconception/antenatal priorities; social determinants; family health; and cultural considerations. These priorities provide a road map to progress research and translation activities in preconception health with future efforts required to advance evidence-translation and implementation to impact clinical outcomes.
Platelet-Rich Plasma as Adjuvant Therapy for Recurrent Vesicovaginal Fistula: A Prospective Case Series
Authors: Streit-Ciećkiewicz Dominika , Futyma Konrad , Miotła Paweł , Grzybowska Magdalena Emilia , Rechberger Tomasz
Published: December 2019

Vesicovaginal fistula (VVF) is the nonphysiological communication between the bladder and vagina, and surgical closure is the gold treatment standard. Despite that successful closure occurs in around 85% of patients after the first repair, recurrence remains a highly distressing complication for patients and surgeons. The aim of our study was to evaluate the efficacy of a platelet-rich plasma (PRP) injection as a supportive treatment in the surgical repair of recurrent VVF. Between January 2018 and July 2019, 16 patients with recurrent VVF were injected with PRP in a tertiary gynecological department. Subsequently, a surgical Latzko procedure for VVF closure was scheduled 6–8 weeks after the PRP injection allowing proper neovascularization and remodeling of surrounding tissues. Patients were considered cured if no leakage was observed after surgery and negative dye test results were indicated at follow-up. All patients who were examined therein remained dry. To the best of our knowledge, this is the first study aiming to assess PRP injections as a supporting treatment prior to surgical procedure for recurrent VVF. Preliminary results are encouraging, and we incorporated this method in our clinical practice. Further reports on a larger group will follow.
Approximate Mortality Risks between Hyperuricemia and Diabetes in the United States
Authors: Chen Po-Hsun , Chen Yu-Wei , Liu Wei-Ju , Hsu Ssu-Wei , Chen Ching-Hsien , Lee Chia-Lin
Published: December 2019

Aim: This study aimed to compare mortality risks across uric acid (UA) levels between non-diabetes adults and participants with diabetes and to investigate the association between hyperuricemia and mortality risks in low-risk adults. Methods: We analyzed data from adults aged >18 years without coronary heart disease and chronic kidney disease (n = 29,226) from the National Health and Nutrition Examination Survey (1999–2010) and the associated mortality data (up to December 2011). We used the Cox proportional hazards models to examine the risk of all-cause and cause-specific (cardiovascular disease (CVD) and cancer) mortality at different UA levels between adults with and without diabetes. Results: Over a median follow-up of 6.6 years, 2069 participants died (495 from CVD and 520 from cancers). In non-diabetes adults at UA ≥ 5 mg/dL, all-cause and CVD mortality risks increased across higher UA levels (p-for-trend = 0.037 and 0.058, respectively). The lowest all-cause mortality risk in participants with diabetes was at the UA level of 5–7 mg/dL. We set the non-diabetes participants with UA levels of <7 mg/dL as a reference group. Without considering the effect of glycemic control, the all-cause mortality risk in non-diabetes participants with UA levels of ≥7 mg/dL was equivalent to risk among diabetes adults with UA levels of <7 mg/dL (hazard ratio = 1.44 vs. 1.57, p = 0.49). A similar result was shown in CVD mortality risk (hazard ratio = 1.80 vs. 2.06, p = 0.56). Conclusion: Hyperuricemia may be an indicator to manage multifaceted cardiovascular risk factors in low-risk adults without diabetes, but further studies and replication are warranted.
The Biomechanics of Pregnancy: A Systematic Review
Authors: Conder Rebecca , Zamani Reza , Akrami Mohammad
Published: December 2019

During pregnancy, a number of biomechanical and hormonal changes occur that can alter spinal curvature, balance, and gait patterns by affecting key areas of the human body. This can greatly impact quality of life (QOL) by increasing back pain and the risk of falls. These effects are likely to be the ultimate result of a number of hormonal and biomechanical changes that occur during pregnancy. Research Question and Methodology: Using the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines, this systematic review sets out to analyse all available literature relating to the biomechanics factors caused by pregnancy and assess how this might reduce QOL. Fifty papers were deemed eligible for inclusion in this review based on the PUBMED and SCOPUS databases. Results: Angles of lordosis and kyphosis of the spine are significantly increased by pregnancy, but not consistently across all studies. Back pain is significantly increased in pregnant women, although this is not significantly correlated with spinal changes. Increased movements of centre of pressure (COP) and increased stability indexes indicate postural control is reduced in pregnancy. Trunk range of motion, hip flexion, and extension are reduced, as well as decreased stride length, decreased gait velocity, and increased step width; again, not consistently. It is likely that each woman adopts unique techniques to minimise the effects, for example increasing step width to improve balance. Further research should focus on how altered limb kinematics during gait might affect QOL by influencing the human body, as well as assessing parameters in all planes to develop a wider understanding of pregnant biomechanical alterations.
Machine Tools Anomaly Detection Through Nearly Real-Time Data Analysis
Authors: Herranz Gorka , Antolínez Alfonso , Escartín Javier , Arregi Amaia , Gerrikagoitia Jon Kepa
Published: December 2019

This work presents a new methodology for machine tools anomaly detection via operational data processing. The previous methodology has been field tested on a milling-boring machine in a real production environment. This paper also describes the data acquisition process, as well as the technical architecture needed for data processing. Subsequently, a technique for operational machine data segmentation based on dynamic time warping and hierarchical clustering is introduced. The formerly mentioned data segmentation and analysis technique allows for machine tools anomaly detection thanks to comparison between near real-time machine operational information, coming from strategically positioned sensors and outcomes collected from previous production cycles. Anomaly detection techniques shown in this article could achieve significant production improvements: “zero-defect manufacturing”, boosting factory efficiency, production plans scrap minimization, improvement of product quality, and the enhancement of overall equipment productivity.
Foundations in Offshore Wind Farms: Evolution, Characteristics and Range of Use. Analysis of Main Dimensional Parameters in Monopile Foundations
Authors: Sánchez Sergio , López-Gutiérrez José-Santos , Negro Vicente , Esteban M. Dolores
Published: December 2019

Renewable energies are the future, and offshore wind is undoubtedly one of the renewable energy sources for the future. Foundations of offshore wind turbines are essential for its right development. There are several types: monopiles, gravity-based structures, jackets, tripods, floating support, etc., being the first ones that are most used up to now. This manuscript begins with a review of the offshore wind power installed around the world and the exposition of the different types of foundations in the industry. For that, a database has been created, and all the data are being processed to be exposed in clear graphic summarizing the current use of the different foundation types, considering mainly distance to the coast and water depth. Later, the paper includes an analysis of the evolution and parameters of the design of monopiles, including wind turbine and monopile characteristics. Some monomials are considered in this specific analysis and also the soil type. So, a general view of the current state of monopile foundations is achieved, based on a database with the offshore wind farms in operation.
Native Osseous CaP Biomineral Coating on a Biomimetic Multi-Spiked Connecting Scaffold Prototype for Cementless Resurfacing Arthroplasty Achieved by Combined Electrochemical Deposition
Authors: Uklejewski Ryszard , Winiecki Mariusz , Krawczyk Piotr , Tokłowicz Renata
Published: December 2019

The multi-spiked connecting scaffold (MSC-Scaffold) prototype with spikes mimicking the interdigitations of articular subchondral bone is an essential innovation in surgically initiated fixation of resurfacing arthroplasty (RA) endoprosthesis components. This paper aimed to present a determination of the suitable range of conditions for the calcium phosphate (CaP) potentiostatic electrochemical deposition (ECDV=const) on the MSC-Scaffold prototype spikes to achieve a biomineral coating with a native Ca/P ratio. The CaP ECDV=const process on the MSC-Scaffold Ti4Al6V pre-prototypes was investigated for potential VECDfrom −9 to −3 V, and followed by 48 h immersion in a simulated body fluid. An acid–alkaline pretreatment (AAT) was applied for a portion of the pre-prototypes. Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) studies of deposited coatings together with coatings weight measurements were performed. Themost suitable VECD range, from −5.25 to −4.75 V, was determined as the native biomineral Ca/P ratio of coatings was achieved. AAT increases the weight of deposited coatings (44% for VECD = −5.25 V, 9% for VECD = −5.00 V and 15% for VECD = −4.75 V) and the coverage degree of the lateral spike surfaces (40% for VECD = −5.25 V, 14% for VECD = −5.00 V and 100% for VECD = −4.75 V). XRD confirmed that the multiphasic CaP coating containing crystalline octacalcium phosphate is produced on the lateral surface of the spikes of the MSC-Scaffold. ECDV=const preceded by AAT prevents micro-cracks on the bone-contacting surfaces of the MSC-Scaffold prototype, increases its spikes’ lateral surface coverage, and results in the best modification effect at VECD = −5.00 V. To conclude, the biomimetic MSC-Scaffold prototype with desired biomineral coating of native Ca/P ratio was obtained for cementless RA endoprostheses.
The Influence of A Cross-Channel Extrusion Process on The Microstructure and Properties of Copper
Authors: Łyszkowski Radosław , Łazińska Magdalena , Zasada Dariusz
Published: December 2019

A new cross-channel extrusion (CCE) method with the application of a back pressure (BP) is proposed and experimentally tested. The introduction of pressure blocks the free flow of material by using an additional set of pistons, which prevents the loss of consistency. The paper presents results of experimental trials of CCE process. Between one and eight passes of CCE with and without a BP were applied to pure copper billets to refine their initial coarse-grained microstructure at room temperature. It was found that processing by CCE results in the formation of a lamellar structure along the extruded axis and the fine-grained structure in the remaining volume. The material exhibited dynamic recrystallization, which results in the formation of 0.5- to 2-μm grains after one pass and 2- to 8-μm grains after four CCE passes. The fine-grained material had YS of 390-415 MPa. An increase in the microhardness from 70 to 130 HV02 after one pass and then a decrease after four passes were observed. This might indicate that secondary recrystallization and selective grain growth occur, because an exothermic peak (158.5 °C, 53 ± 2.1 J/mol) was observed during DSC (differential scanning calorimetry) testing. The resistivity of the once deformed copper significantly decreases, while its further processing causes the resistivity to increase.
Thermophysical Measurements in Liquid Alloys and Phase Diagram Studies
Authors: Kirshon Yuri , Ben Shalom Shir , Emuna Moran , Greenberg Yaron , Lee Joonho , Makov Guy , Yahel Eyal
Published: December 2019

Towards the construction of pressure-dependent phase diagrams of binary alloy systems, both thermophysical measurements and thermodynamic modeling are employed. High-accuracy measurements of sound velocity, density, and electrical resistivity were performed for selected metallic elements from columns III to V and their alloys in the liquid phase. Sound velocity measurements were made using ultrasonic techniques, density measurements using the gamma radiation attenuation method, and electrical resistivity measurements were performed using the four probe method. Sound velocity and density data, measured at ambient pressure, were incorporated into a thermodynamic model to calculate the pressure dependence of binary phase diagrams. Electrical resistivity measurements were performed on binary systems to study phase separation and identify phase transitions in the liquid state.
PMMA-Based Bone Cements and the Problem of Joint Arthroplasty Infections: Status and New Perspectives
Authors: Bistolfi Alessandro , Ferracini Riccardo , Albanese Carlo , Vernè Enrica , Miola Marta
Published: December 2019

Polymethyl methacrylate (PMMA)-based bone cement is a biomaterial that has been used over the last 50 years to stabilize hip and knee implants or as a bone filler. Although PMMA-based bone cement is widely used and allows a fast-primary fixation to the bone, it does not guarantee a mechanically and biologically stable interface with bone, and most of all it is prone to bacteria adhesion and infection development. In the 1970s, antibiotic-loaded bone cements were introduced to reduce the infection rate in arthroplasty; however, the efficiency of antibiotic-containing bone cement is still a debated issue. For these reasons, in recent years, the scientific community has investigated new approaches to impart antibacterial properties to PMMA bone cement. The aim of this review is to summarize the current status regarding antibiotic-loaded PMMA-based bone cements, fill the gap regarding the lack of data on antibacterial bone cement, and explore the progress of antibacterial bone cement formulations, focusing attention on the new perspectives. In particular, this review highlights the innovative study of composite bone cements containing inorganic antibacterial and bioactive phases, which are a fascinating alternative that can impart both osteointegration and antibacterial properties to PMMA-based bone cement.
Effects of Temperature on the Tribological Properties of NM600 under Sliding Wear
Authors: Pei Yingchao , Xia Dianxiu , Wang Shouren , Cong Liang , Wang Xuelin , Wang Dongyue
Published: December 2019

An investigation on the tribological properties of GCr15 sliding against NM600 was carried out using a high-temperature friction and wear tester. As the temperature rose from room temperature to 300 °C, the average friction coefficient of NM600 increased rapidly, then decreased rapidly, and then became stable. The wear volume and specific wear rate of NM600 increased rapidly, then decreased rapidly, and then increased slowly. The wear mechanism and matrix properties of the tested steel at different temperatures are the main reasons for the above results. At 20–50 °C, the main wear mechanism was adhesive wear, fatigue wear, and abrasive wear. At 100–150 ℃, the wear mechanism was mainly adhesive wear, fatigue wear, abrasive wear, and oxidation wear. At 200–300 °C, the wear mechanism was mainly oxidation wear and abrasive wear.
Fast Computation of Integrals with Fourier-Type Oscillator Involving Stationary Point
Authors: Zaman Sakhi , Hussain Irshad , Singh Dhananjay
Published: December 2019

An adaptive splitting algorithm was implemented for numerical evaluation of Fourier-type highly oscillatory integrals involving stationary point. Accordingly, a modified Levin collocation method was coupled with multi-resolution quadratures in order to tackle the stationary point and irregular oscillations of the integrand caused by ω . Some test problems are included to verify the accuracy of the proposed methods.
Enhancement of Deep Learning in Image Classification Performance Using Xception with the Swish Activation Function for Colorectal Polyp Preliminary Screening
Authors: Jinsakul Natinai , Tsai Cheng-Fa , Tsai Chia-En , Wu Pensee
Published: December 2019

One of the leading forms of cancer is colorectal cancer (CRC), which is responsible for increasing mortality in young people. The aim of this paper is to provide an experimental modification of deep learning of Xception with Swish and assess the possibility of developing a preliminary colorectal polyp screening system by training the proposed model with a colorectal topogram dataset in two and three classes. The results indicate that the proposed model can enhance the original convolutional neural network model with evaluation classification performance by achieving accuracy of up to 98.99% for classifying into two classes and 91.48% for three classes. For testing of the model with another external image, the proposed method can also improve the prediction compared to the traditional method, with 99.63% accuracy for true prediction of two classes and 80.95% accuracy for true prediction of three classes.
Insignificant Impact of Chemotactic Responses of Pseudomonas aeruginosa on the Bacterial Attachment to Organic Pre-Conditioned RO Membranes
Authors: Kim Lan Hee , Vrouwenvelder Johannes S.
Published: December 2019

We investigated the impact of conditioning compositions on the way bacteria move and adhere to reverse osmosis (RO) membranes that have been pre-conditioned by organic compounds. We used humic acid (HA), bovine serum albumin (BSA), and sodium alginate (SA) to simulate conditioning layers on the RO membranes. First, we investigated the chemotactic responses of Pseudomonas aeruginosa PAO1 to the organic substances and the impact of changes in physicochemical characteristics of pre-conditioned membranes on bacterial attachment. Second, we observed bacterial attachment under the presence or absence of nutrients or microbial metabolic activity. Results showed that there was no relationship between the chemotactic response of P. aeruginosa PAO1 and the organic substances, and the changes in hydrophobicity, surface free energy, and surface charge resulting from changing the composition of the conditioning layer did not seem to affect bacterial attachment, whereas changing the roughness of the conditioned membrane exponentially did (exponential correlation coefficient, R2 = 0.85). We found that the initial bacterial attachment on the membrane surface is influenced by (i) the nutrients in the feed solution and (ii) the microbial metabolic activity, whereas the chemotaxis response has a negligible impact. This study would help to establish a suitable strategy to manage bacterial attachment.
Current State of Semi-Solid Net-Shape Die Casting
Authors: Kapranos Plato
Published: December 2019

Semi-solid processing of alloys is nearing the end of its fifth decade in existence. This promising manufacturing route has undergone a number of changes over the past five decades and apart from certain successful industrial applications, it appeared that it had become a niche technology with limited applications, notably in the automotive and consumer product markets. Nevertheless, despite the strong competition of traditional casting and in particular die-casting processes, and the emergence of Additive Manufacturing (AM), there seems to be a resurgence in demand for Net-Shape Die Casting of Semi-solid alloys as testified by the high numbers of delegates at the 15th SSM Conference at Shenzhen, China in September 2018. What follows is a brief review of the historical development of the process as well as more recent developments with an eye to future prospects.
A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly
Authors: Sha Xiaopeng , Sun Hui , Zhao Yuliang , Li Wenchao , Li Wen J.
Published: December 2019

Micromanipulation is an interdisciplinary technology that integrates advanced knowledge of microscale/nanoscale science, mechanical engineering, electronic engineering, and control engineering. Over the past two decades, it has been widely applied in the fields of MEMS (microelectromechanical systems), bioengineering, and microdevice integration and manufacturing. Microvision servoing is the basic tool for enabling the automatic and precise micromanipulation of microscale/nanoscale entities. However, there are still many problems surrounding microvision servoing in theory and the application of this technology’s micromanipulation processes. This paper summarizes the research, development status, and practical applications of critical components of microvision servoing for micromanipulation, including geometric calibration, autofocus techniques, depth information, and visual servoing control. Suggestions for guiding future innovation and development in this field are also provided in this review.
The Amyloid as a Ribbon-Like Micelle in Contrast to Spherical Micelles Represented by Globular Proteins
Authors: Banach Mateusz , Konieczny Leszek , Roterman Irena
Published: December 2019

Selected amyloid structures available in the Protein Data Bank have been subjected to a comparative analysis. Classification is based on the distribution of hydrophobicity in amyloids that differ with respect to sequence, chain length, the distribution of beta folds, protofibril structure, and the arrangement of protofibrils in each superfibril. The study set includes the following amyloids: Aβ (1–42), which is listed as Aβ (15–40) and carries the D23N mutation, and Aβ (11–42) and Aβ (1–40), both of which carry the E22Δ mutation, tau amyloid, and α-synuclein. Based on the fuzzy oil drop model (FOD), we determined that, despite their conformational diversity, all presented amyloids adopt a similar structural pattern that can be described as a ribbon-like micelle. The same model, when applied to globular proteins, results in structures referred to as “globular micelles,” emerging as a result of interactions between the proteins’ constituent residues and the aqueous solvent. Due to their composition, amyloids are unable to attain entropically favorable globular forms and instead attempt to limit contact between hydrophobic residues and water by producing elongated structures. Such structures typically contain quasi hydrophobic cores that stretch along the fibril’s long axis. Similar properties are commonly found in ribbon-like micelles, with alternating bands of high and low hydrophobicity emerging as the fibrils increase in length. Thus, while globular proteins are generally consistent with a 3D Gaussian distribution of hydrophobicity, the distribution instead conforms to a 2D Gaussian distribution in amyloid fibrils.
Some Properties of Electron Beam-Irradiated Sheep Wool Linked to Cr(III) Sorption
Authors: Braniša Jana , Kleinová Angela , Jomová Klaudia , Malá Radka , Morgunov Volodymyr , Porubská Mária
Published: December 2019

We examined the characteristics of an electron beam irradiated wool with an absorbed dose of (21–410) kGy in comparison with natural wool with respect to the determination of the isoelectric point (IEP), zero charge point (ZCP), mechanism of Cr(III) sorption from higher concentrated solutions, and the modelling of the wool-Cr(III) interaction. The data of ZPC and IEP differed between natural and irradiated samples. Increasing the dose shifted the pH of ZPC from 6.85 for natural wool to 6.20 for the highest dosed wool, while the natural wool IEP moved very little, from pH = 3.35 to 3.40 for all of the irradiated samples. The sorption experiments were performed in a pH bath set at 3.40, and the determination of the residual Cr(III) in the bath was performed by VIS spectrometry under optimized conditions. The resulting sorptivity showed a monotonically rising trend with increasing Cr(III) concentration in the bath. Lower doses, unlike higher doses, showed better sorptivity than the natural wool. FTIR data indicated the formation of complex chromite salts of carboxylates and cysteinates. Crosslinks via ligands coming from different keratin chains were predicted, preferably on the surface of the fibers, but to a degree that did not yet inhibit the diffusion of Cr(III)-cations into the fiber volume. We also present a concept of a complex octahedral structure.
Synthesis and Biological Evaluation of BODIPY-PF-543
Authors: Shrestha Jitendra , Hwang Gil Tae , Lee Taeho , Kim Seon Woong , Oh Yoon Sin , Kwon Yongseok , Hong Seung Woo , Kim Sanghee , Seop Moon Hong , Baek Dong Jae , Park Eun-Young
Published: December 2019

Sphingosine-1-phosphate (S1P) regulates the proliferation of various cells and promotes the growth of cancer cells. Sphingosine kinase (SK), which transforms sphingosine into S1P, has two isotypes: SK1 and SK2. To date, both isotypes are known to be involved in the proliferation of cancer cells. PF-543, an SK1 inhibitor developed by Pfizer, strongly inhibits SK1. However, despite its strong SK1 inhibitory effect, PF-543 shows low anticancer activity in vitro. Therefore, additional biological evidence on the anticancer activity of SK1 inhibitor is required. The present study aimed to investigate the intracellular localization of PF-543 and identify its association with anticancer activity by introducing a fluoroprobe into PF-543. Boron–dipyrromethene (BODIPY)-introduced PF-543 has a similar SK1 inhibitory effect as PF-543. These results indicate that the introduction of BODIPY does not significantly affect the inhibitory effect of SK1. In confocal microscopy after BODIPY-PF-543 treatment, the compound was mainly located in the cytosol of the cells. This study demonstrated the possibility of introducing fluorescent material into an SK inhibitor and designing a synthesized compound that is permeable to cells while maintaining the SK inhibitory effect.
Preparation of pH Responsive Polystyrene and Polyvinyl Pyridine Nanospheres Stabilized by Mickering Microgel Emulsions
Authors: Atta Ayman M. , Ezzat Abdelrahman O. , Al-Lohedan Hamad A. , Tawfeek Ahmed M. , Alobaidi Abdulaziz A.
Published: December 2019

New pH-sensitive polystyrene, PS, and poly(4-vinylpyridine), P4-VP, nanospheres were prepared by using surfactant-free method based on soft microgels (Mickering emulsion). The formation of stable Mickering cyclohexane/water emulsions was investigated by using soft microgel particles of poly(acrylamide), PAAm, poly(2-acrylamido-2-methylpropane sulfonic acid), PAMPS, and sodium salt of PAMPS, PAMPS-Na, as stabilizers. The dynamic light scattering (DLS), optical microscopy, and scanning electron microscopy (SEM) were used to investigate the optimum conditions and effects of surrounding solutions on the microgels characteristics and their corresponding Mickering emulsions. The cyclohexane/water Mickering emulsions stabilized by softer and neutral charged microgels were considerably more stable under the same conditions. Furthermore, the stimuli-responsive properties of PAMPS microgel stabilized cyclohexane/water Mickering emulsions suggest the potential utility in the preparation of PS and P4-VP nanospheres. The effects of pH changes on the morphology, particle sizes, and surface charges of PS and P4-VP microgels were evaluated to prove the pH-sensitivity of the prepared nanospheres.
Non-Linear Optical Effects in Nanomaterials
Authors: Mysliwiec Jaroslaw
Published: December 2019

Nonlinear optics is the domain of optics that studies the physical interaction between one or multiple optical beams of high intensity and an optical medium [...]
Reactive Mesoporous pH-Sensitive Amino-Functionalized Silica Nanoparticles for Efficient Removal of Coomassie Blue Dye
Authors: Sabeela Nourah I. , Almutairi Tahani M. , Al-Lohedan Hamad A. , Ezzat Abdelrahman O. , Atta Ayman M.
Published: December 2019

In this work, new smart mesoporous amine-functionalized silica nanoparticles were prepared from hydrolyzing microgels based on N-isopropyl acrylamide-co-vinyltrimethoxysilane microgels with tetraethoxysilicate and 3-aminopropyltriethoxysilane by sol-gel method. The thermal stability and Fourier transform infrared were used to determine the amine contents of the silica nanoparticles. The pH sensitivity of the synthesized silica nanoparticles in their aqueous solutions was evaluated by using dynamic light scattering (DLS) and zeta potential measurements. The porosity of the amine-functionalized silica nanoparticles was evaluated from a transmittance electron microscope and Brunauer-Emmett-Teller (BET) plot. The results have positively recommended the pH-sensitive amine-functionalized silica nanoparticles as one of the effective nano-adsorbent to remove 313 mg·g−1 of CB-R250 water pollutant.