bluuebunny/crossref_metadata_2025 huggingface dataset:
Case III.4: Community Engagement: Cultivating Critical Awareness
Authors: Crowther Jim , Shaw Mae
Published: May 2021

We are writing as teachers and academics with substantial experience over many years (50 years combined) on undergraduate and postgraduate programmes of professional community education. This chapter is derived from that experience and developed from Community Engagement: A Critical Guide for Practitioners (Shaw and Crowther, 2017...
The Currency of Distributive Justice
Authors: Wienhues Anna
Published: May 2021

This chapter examines ecological space as a suitable currency of distributive justice by analysing a range of different definitions of the concept. Ecological space is originally defined as comprising 'all the environmental goods and natural resources that play a part in the socio-economic life of humankind'. The chapter surveys how the concept of ecological space has been used in environmental political theory and how it relates to similar concepts. Based on this, it is possible to propose that green theories of justice should understand ecological space as the (potential) benefits provided by the Earth's life-support systems and physical resources such as land, and non-renewable and renewable natural resources. The chapter then explains why this is an appropriate distribuendum for green theories of justice and indicates how such an understanding of ecological space might be operationalised in order to assess real world distribution problems.
Ecological Justice and the Capabilities Approach
Authors: Wienhues Anna
Published: May 2021

This chapter presents an alternative theoretical framework for grounding ecological justice: the capabilities approach. Rather than focusing on the distribution of some material goods themselves, the focus of the capabilities approach lies on the functionings — that is, doings and beings — and the capabilities — that is, opportunities or freedoms to achieve these functionings — of humans. The provision of these capabilities, which require different inputs depending on the individual in question, are at the heart of its concern. In other words, capability theorists are concerned with the opportunities that individuals need to live fully functioning — or flourishing — lives. On first examination, the capabilities approach has a lot of intuitive force in the domain of ecological justice, arguably because of its close connection to the concepts of needs and flourishing. However, accounts of interspecies justice based on the capabilities approach have been met with powerful criticism which leads to the conclusion that the project of expanding the capabilities approach into the nonhuman sphere will require considerable adjustments, and consequently the task of developing interspecies justice should rather be left to less anthropomorphist approaches.
Outlook for Implementation
Authors: Wienhues Anna
Published: May 2021

This chapter explores some implications of the book's framework in terms of how to implement and discharge duties of ecological justice, as for example in terms of ecological citizenship. The book presents an account of global non-ranking biocentric distributive ecological/interspecies justice to wild nonhuman beings. Based on this theoretical framework, the human takeover of the Earth's ecological space — its resources, ecosystem benefits, and actual spaces — that ultimately leads to species extinctions constitutes an injustice; it should be discussed and responded to as a matter of justice. Duties of global justice are held collectively by humanity and thus need to be acted upon collectively via political institutions. But these duties are not held equally globally due to different historical and present responsibilities for the injustices at hand. Discharging duties of distributive interspecies justice becomes a matter of just implementation which in turn is driven by intra-social justice considerations.
Introduction to Part II
Authors: Robé Jean-Philippe
Published: May 2021

The second part of this book explains the position of global firms in the World Power System and how a renewed understanding of the effective structure of the Power System can lead to an adjustment of their role. Today’s World Power System is facing obvious difficulties to address potentially deadly issues such as climate change. This is in part due to the globalization of firms and of value chains which make it almost impossible for States to fulfil their internalizing and redistributive functions. States are now in the uncomfortable position of having to behave both as the whole and as the part, as the container and the content, as the inclusive instance of power and as a mere component included in a larger Power System....
Laser frequency stabilization via bichromatic Doppler-free spectroscopy
of an 87Rb D1 line
Authors: Zhao Minhua , Jiang Xunda , Fang Ruihuan , Qiu Yuxiang , Ma Zhu , Han Chengyin , Lu Bo , Lee Chaohong
Published: May 2021

We demonstrate a bichromatic Doppler-free spectroscopy of an

87

R
b

D

1

line by using a dual-frequency,
counterpropagating laser field with orthogonal linear polarizations. A
reversed Doppler-free resonance dip is observed in the dual-frequency
scheme, and a significant improvement of frequency discrimination
curve is acquired due to the coherent population trapping (CPT)
effect. The influence of the static magnetic field and laser intensity
on the spectroscopy is studied in both single- and dual-frequency
schemes. After locking the laser frequency to the

87

R
b

D

1

line in the dual-frequency
stabilization scheme, the beat note fractional frequency stability is
at the level of

7
×

10

−
12

at 1 s integration time. This
technique can be used in various applications, such as CPT atomic
clocks, laser spectroscopy, quantum optics, and laser-cooling
experiments.
Experimental investigation of 16.6 Gbps SDM-WDM visible light communication based on a neural network receiver and tricolor mini-LEDs
Authors: Liu Xin , Wei Zixian , Li Mutong , Wang Lei , Liu Zhongxu , Yu Changyuan , Wang Lai , Luo Yi , Fu H. Y.
Published: May 2021

Visible light communication (VLC) based on a light-emitting diode (LED) suffers from a limited bandwidth of commercial LED, device nonlinearity, channel distortion, and transmitted power caused by a complex free-space channel, power amplifier, and illuminant devices resulting in a limited data rate. In this Letter, to provide an alternative high-speed solution, we first designed and fabricated three 175 µm tricolor mini-LEDs with various wavelengths. They are used to set up a spatial division multiplexing-wavelength division multiplexing VLC system over a 2 m link. Then we utilize a neural network receiver to replace the traditional channel estimation, equalization, and demodulation at the offline digital signal processing of the receiver. The experiment showed that the data rates of 2.65, 7, and 7 Gbps were achieved in three respective links using quadrature phase shift keying-optical orthogonal frequency division multiplexing. The three data rates have bit-error rates below the forward error correction limit, whose data rate sum of 16.6 Gbps is the highest mini/micro-LED-based VLC, to the best of our knowledge.
VCF2CAPS–A high-throughput CAPS marker design from VCF files and its test-use on a genotyping-by-sequencing (GBS) dataset
Authors: Wesołowski Wojciech , Domnicz Beata , Augustynowicz Joanna , Szklarczyk Marek
Published: May 2021

Next-generation sequencing (NGS) is a powerful tool for massive detection of DNA sequence variants such as single nucleotide polymorphisms (SNPs), multi-nucleotide polymorphisms (MNPs) and insertions/deletions (indels). For routine screening of numerous samples, these variants are often converted into cleaved amplified polymorphic sequence (CAPS) markers which are based on the presence versus absence of restriction sites within PCR products. Current computational tools for SNP to CAPS conversion are limited and usually infeasible to use for large datasets as those generated with NGS. Moreover, there is no available tool for massive conversion of MNPs and indels into CAPS markers. Here, we present VCF2CAPS–a new software for identification of restriction endonucleases that recognize SNP/MNP/indel-containing sequences from NGS experiments. Additionally, the program contains filtration utilities not available in other SNP to CAPS converters–selection of markers with a single polymorphic cut site within a user-specified sequence length, and selection of markers that differentiate up to three user-defined groups of individuals from the analyzed population. Performance of VCF2CAPS was tested on a thoroughly analyzed dataset from a genotyping-by-sequencing (GBS) experiment. A selection of CAPS markers picked by the program was subjected to experimental verification. CAPS markers, also referred to as PCR-RFLPs, belong to basic tools exploited in plant, animal and human genetics. Our new software–VCF2CAPS–fills the gap in the current inventory of genetic software by high-throughput CAPS marker design from next-generation sequencing (NGS) data. The program should be of interest to geneticists involved in molecular diagnostics. In this paper we show a successful exemplary application of VCF2CAPS and we believe that its usefulness is guaranteed by the growing availability of NGS services.
Genomic analysis for the prediction of prognosis in small-bowel cancer
Authors: Tsuboi Akiyoshi , Urabe Yuji , Oka Shiro , Sumioka Akihiko , Iio Sumio , Yuge Ryo , Hayashi Ryohei , Kuwai Toshio , Kitadai Yasuhiko , Kuraoka Kazuya , Arihiro Koji , Tanaka Shinji , Chayama Kazuaki
Published: May 2021

The current understanding of clinicopathological features and genomic variants of small-bowel cancer is limited, in part due to the rarity of the disease. However, understanding of these factors is necessary for the development of novel therapeutic agents for small-bowel cancer. Thus, we aimed to identify the clinicopathological features and genomic variants associated with its prognosis and recurrence. We retrospectively examined 24 consecutive patients with primary small-bowel cancer surgically treated between May 2005 and August 2018 and collected 29 tumor specimens. The 29 lesions were subjected to mismatch repair status evaluation, using immunohistochemistry (IHC), and targeted genomic sequencing, after which they were analyzed using a panel of 90 cancer-related genes. IHC revealed that 45% (13/29) of the lesions exhibited deficient mismatch repair. The most common genomic variants in small-bowel cancers were in TP53 (48%, 13/27), followed by KRAS (44%, 12/27), ARID1A (33%, 9/27), PIK3CA (26%, 7/27), APC (26%, 7/27), and SMAD4, NOTCH3, CREBBP, PTCH1, and EP300 (22%, 6/27 each). Overall survival and disease-specific survival of patients with tumor mutational burden (TMB) ≥10 mutations/Mb (n = 17) were significantly better than those of patients with TMB <10 mutations/Mb (n = 6). Additionally, patients with a mutant SMAD4 had poorer recurrence-free survival than those with wild-type SMAD4. Our results suggested that TMB and SMAD4 mutations were associated with the prognosis of small-bowel cancer patients. Thus, cancer genomic analysis could be useful in the search for biomarkers of prognosis prediction in small-bowel cancers.
Spatial Congruence Analysis (SCAN): A method for detecting biogeographical patterns based on species range congruences
Authors: Gatto Cassiano A. F. R. , Cohn-Haft Mario
Published: May 2021

Species with congruent geographical distributions, potentially caused by common historical and ecological spatial processes, constitute biogeographical units called chorotypes. Nevertheless, the degree of spatial range congruence characterizing these groups of species is rarely used as an explicit parameter. Methods conceived for the identification of patterns of shared ranges often suffer from scale bias associated with the use of grids, or the incapacity to describe the full complexity of patterns, from core areas of high spatial congruence, to long gradients of range distributions expanding from these core areas. Here, we propose a simple analytical method, Spatial Congruence Analysis (SCAN), which identifies chorotypes by mapping direct and indirect spatial relationships among species. Assessments are made under a referential value of congruence as an explicit numerical parameter. A one-layered network connects species (vertices) using pairwise spatial congruence estimates (edges). This network is then analyzed for each species, separately, by an algorithm which searches for spatial relationships to the reference species. The method was applied to two datasets: a simulated gradient of ranges and real distributions of birds. The simulated dataset showed that SCAN can describe gradients of distribution with a high level of detail. The bird dataset showed that only a small portion of range overlaps is biogeographically meaningful, and that there is a large variation in types of patterns that can be found with real distributions. Species analyzed separately may converge on similar or identical groups, may be nested in larger chorotypes, or may even generate overlapped patterns with no species in common. Chorotypes can vary from simple ones, composed by few highly congruent species, to complex, with numerous alternative component species and spatial configurations, which offer insights about possible processes driving these patterns in distinct degrees of spatial congruence. Metrics such as congruence, depth, richness, and ratio between common and total areas can be used to describe chorotypes in detail, allowing comparisons between patterns across regions and taxa.
Comparison the effects of carotid endarterectomy with carotid artery stenting for contralateral carotid occlusion
Authors: Sun Yaxuan , Ding Yongxia , Meng Kun , Han Bin , Wang Jing , Han Yan
Published: May 2021

Background

There have been inconsistent results regarding the use of carotid artery endarterectomy (CEA) versus carotid artery stenting (CAS) for contralateral carotid occlusion (CCO). This study aimed to determine the optimal revascularization technique for patients with CCO.

Methods

We systematically searched the PubMed, Embase, and Cochrane Library databases to identify eligible studies published from inception to January 2, 2021. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to calculate pooled effect estimates using a random-effects model. Sensitivity, subgroup, and publication bias analyses were also performed.

Results

Six studies involving 6,953 patients were selected for inclusion in this meta-analysis. Our results showed that while CEA was not associated with an increased risk of stroke compared to CAS (OR: 1.07; 95% CI: 0.75–1.51; P = 0.713), CEA was associated with a reduced risk of death compared to CAS (OR: 0.45; 95% CI: 0.29–0.70; P < 0.001). Furthermore, there were no significant differences between CEA and CAS for the risks of myocardial infarction (OR: 1.38; 95% CI: 0.73–2.62; P = 0.319) or major adverse cardiovascular events (OR: 1.03; 95% CI: 0.56–1.88; P = 0.926). Finally, the risk of myocardial infarction for CEA versus CAS was affected by disease status, while the risk of major adverse cardiovascular events was affected by the proportions of patients with male gender, coronary artery disease, and current or prior smoking.

Conclusion

This study found that CEA and CAS resulted in similar outcomes for patients with CCO, while the risk of death was reduced in patients treated with CEA. Further high-level evidence should be collected to verify the results of this study.
Genotypic diversity and plasticity of root system architecture to nitrogen availability in oilseed rape
Authors: Lecarpentier Christophe , Pagès Loïc , Richard-Molard Céline
Published: May 2021

In the emerging new agricultural context, a drastic reduction in fertilizer usage is required. A promising way to maintain high crop yields while reducing fertilizer inputs is to breed new varieties with optimized root system architecture (RSA), designed to reach soil resources more efficiently. This relies on identifying key traits that underlie genotypic variability and plasticity of RSA in response to nutrient availability. The aim of our study was to characterize the RSA plasticity in response to nitrogen limitation of a set of contrasted oilseed rape genotypes, by using the ArchiSimple model parameters as screening traits. Eight accessions of Brassica napus were grown in long tubes in the greenhouse, under two contrasting levels of nitrogen availability. After plant excavation, roots were scanned at high resolution. Six RSA traits relative to root diameter, elongation rate and branching were measured, as well as nine growth and biomass allocation traits. The plasticity of each trait to nitrogen availability was estimated. Nitrogen-limited plants were characterized by a strong reduction in total biomass and leaf area. Even if the architecture traits were shown to be less plastic than allocation traits, significant nitrogen and genotype effects were highlighted on each RSA trait, except the root minimal diameter. Thus, the RSA of nitrogen-limited plants was primarily characterised by a reduced lateral root density, a smaller primary root diameter, associated with a stronger root dominance. Among the RSA traits measured, the inter-branch distance showed the highest plasticity with a level of 70%, in the same range as the most plastic allocation traits. This work suggests that lateral root density plays the key role in the adaptation of the root system to nitrogen availability and highlights inter-branch distance as a major target trait for breeding new varieties, better adapted to low input systems.
An examination of school reopening strategies during the SARS-CoV-2 pandemic
Authors: Landeros Alfonso , Ji Xiang , Lange Kenneth , Stutz Timothy C. , Xu Jason , Sehl Mary E. , Sinsheimer Janet S.
Published: May 2021

The SARS-CoV-2 pandemic led to closure of nearly all K-12 schools in the United States of America in March 2020. Although reopening K-12 schools for in-person schooling is desirable for many reasons, officials understand that risk reduction strategies and detection of cases are imperative in creating a safe return to school. Furthermore, consequences of reclosing recently opened schools are substantial and impact teachers, parents, and ultimately educational experiences in children. To address competing interests in meeting educational needs with public safety, we compare the impact of physical separation through school cohorts on SARS-CoV-2 infections against policies acting at the level of individual contacts within classrooms. Using an age-stratified Susceptible-Exposed-Infected-Removed model, we explore influences of reduced class density, transmission mitigation, and viral detection on cumulative prevalence. We consider several scenarios over a 6-month period including (1) multiple rotating cohorts in which students cycle through in-person instruction on a weekly basis, (2) parallel cohorts with in-person and remote learning tracks, (3) the impact of a hypothetical testing program with ideal and imperfect detection, and (4) varying levels of aggregate transmission reduction. Our mathematical model predicts that reducing the number of contacts through cohorts produces a larger effect than diminishing transmission rates per contact. Specifically, the latter approach requires dramatic reduction in transmission rates in order to achieve a comparable effect in minimizing infections over time. Further, our model indicates that surveillance programs using less sensitive tests may be adequate in monitoring infections within a school community by both keeping infections low and allowing for a longer period of instruction. Lastly, we underscore the importance of factoring infection prevalence in deciding when a local outbreak of infection is serious enough to require reverting to remote learning.
High-frequency conductivity at Larmor-frequency in human brain using moving local window multilayer perceptron neural network
Authors: Lee Mun Bae , Jahng Geon-Ho , Kim Hyung Joong , Kwon Oh-In
Published: May 2021

Magnetic resonance electrical properties tomography (MREPT) aims to visualize the internal high-frequency conductivity distribution at Larmor frequency using the B1 transceive phase data. From the magnetic field perturbation by the electrical field associated with the radiofrequency (RF) magnetic field, the high-frequency conductivity and permittivity distributions inside the human brain have been reconstructed based on the Maxwell’s equation. Starting from the Maxwell’s equation, the complex permittivity can be described as a second order elliptic partial differential equation. The established reconstruction algorithms have focused on simplifying and/or regularizing the elliptic partial differential equation to reduce the noise artifact. Using the nonlinear relationship between the Maxwell’s equation, measured magnetic field, and conductivity distribution, we design a deep learning model to visualize the high-frequency conductivity in the brain, directly derived from measured magnetic flux density. The designed moving local window multi-layer perceptron (MLW-MLP) neural network by sliding local window consisting of neighboring voxels around each voxel predicts the high-frequency conductivity distribution in each local window. The designed MLW-MLP uses a family of multiple groups, consisting of the gradients and Laplacian of measured B1 phase data, as the input layer in a local window. The output layer of MLW-MLP returns the conductivity values in each local window. By taking a non-local mean filtering approach in the local window, we reconstruct a noise suppressed conductivity image while maintaining spatial resolution. To verify the proposed method, we used B1 phase datasets acquired from eight human subjects (five subjects for training procedure and three subjects for predicting the conductivity in the brain).
Current status of community-acquired infection of COVID-19 in delivery facilities in Japan
Authors: Hasegawa Junichi , Arakaki Tatsuya , Sekizawa Akihiko , Ikeda Tomoaki , Ishiwata Isamu , Kinoshita Katsuyuki ,   on behalf of department of medical safety, the Japan Association of Obstetricians and Gynecologists (JAOG)
Published: May 2021

A nationwide questionnaire survey about community-acquired infection of coronavirus disease 2019 (COVID-19) was conducted in July 2020 to identify the characteristics of and measures taken by Japanese medical facilities providing maternity services. A case-control study was conducted by including medical facilities with (Cases) and without (Control) community-acquired infection of COVID-19. Responses from 711 hospitals and 707 private clinics were assessed (72% of all hospital and 59% all private clinics provided maternity service in Japan). Seventy-five COVID-19-positive pregnant women were treated in 52 facilities. Community-acquired infection was reported in 4.1% of the facilities. Of these, 95% occurred in the hospital. Nine patients developed a community-acquired infection in the maternity ward or obstetric department. Variables that associated with community-acquired infection of COVID-19 (adjusted odds ratio [95% confidence interval]) were found to be state of emergency prefecture (4.93 [2.17–11.16]), PCR test for SARS-CoV-2 on admission (2.88 [1.59–5.24]), and facility that cannot treat COVID-19 positive patients (0.34 [0.14–0.82]). In conclusion, community-acquired infection is likely to occur in large hospitals that treat a higher number of patients than private clinics do, regardless of the preventive measures used.
Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii
Authors: Zhang Mengying , Chen Xiajun , Zhang Jinpan , Li Jiale , Bai Zhiyi
Published: May 2021

Creb (Cyclic AMP response element binding protein) is a nuclear regulatory factor that regulates transcription through autophosphorylation. In melanocytes, cAMP’s corresponding elements bind to the Creb protein to autophosphorylation and activate MITF (Microphthalmia-associated transcription factor). MITF stimulates Tyrosine(tyr) to induce melanocytes to differentiate into eumelanin and pheomelanin. In this study, a HcCreb gene in Hyriopsis cumingii was cloned and its effects on melanin synthesis and nacre color were studied. HcCreb was expressed in both purple and white mussels, and there was a significant difference in expression between adductor muscle (p<0.01) and mantle tissue (p<0.05). Other tissues did not show significant differences (except for gill tissue), and in general, the level of mRNA expression was higher in purple mussels than in white mussels. In both white and purple mussels expression levels in gill tissue was the highest, followed by the mantle. Strong and specific mRNA signals were detected in the dorsal epithelial cells of the mantle pallial layer, indicating that HcCreb may be involved in nacre formation. After arbutin treatment, the expression of HcCreb decreased significantly. By further testing the changes in mantle melanin content it was found that the melanin content after arbutin treatment decreased significantly compared to the control group (p<0.05). It is speculated that the HcCreb gene plays a role in the process of melanin synthesis and nacre color formation in H. cumingii.
Impact of varicella vaccine on nosocomial outbreaks and management of post exposure prophylaxis following in a paediatric hospital
Authors: Gentile Angela , Giglio Norberto , Lucion Maria Florencia , Martínez Ana Clara , Pejito Natalia , Juarez Maria del Valle
Published: May 2021

Introduction

In 2015, varicella vaccine was introduced to the National Immunization Programme in a one-dose regimen for infants aged 15 months. The aim of this study was to describe and compare the epidemiologic characteristics, management strategies and costs of varicella outbreaks in Ricardo Gutierrez Children’s Hospital (HNRG) from 2000 to 2019, before (PreV period) and after (PostV period) the introduction of the varicella vaccine.

Methods

A retrospective, analytic study of the impact of nosocomial varicella outbreaks at the HNRG, based on active epidemiologic surveillance. We compared nosocomial varicella outbreaks rates (per 10,000 discharges) between PreV and PostV, excluding the intervention year (2015).

Results

During PreV, an average of 15.87 (13.91–18.02) outbreaks per year was observed and in PostV 5.5 per year (3.44–8.32). Outbreaks adjusted by all cause discharges showed a reduction of 59.13% (-36.68%, -73.62%) after vaccine introduction.

Considering that in PreV the average of susceptible cases per outbreak was 5.0 and in PostV 7.8, with a cost per susceptible of AR$ $6,522 (80.27 USD) PreV and 6,708 PostV the economic impact on the reduction of outbreaks after the introduction of the vaccine, showed an estimated average savings per year of AR$ -252,128 AR$ (-3,103.11 USD).

Conclusions

The number of annual varicella hospital outbreaks at the HNRG decreased significantly after varicella vaccine was introduced to NIP in Argentina with a relevant reduction in terms of costs.
Shifting trends: Detecting changes in cetacean population dynamics in shifting habitat
Authors: Boyd Charlotte , Punt André E.
Published: May 2021

The ability to monitor population dynamics and detect major changes in population trend is essential for wildlife conservation and management. However, this is often challenging for cetaceans as surveys typically cover only a portion of a population’s range and conventional stock assessment methods cannot then distinguish whether apparent changes in abundance reflect real changes in population size or shifts in distribution. We developed and tested methods for estimating population size and trend and detecting changes in population trend in the context of shifting habitat by integrating additional data into distance-sampling analysis. Previous research has shown that incorporating habitat information can improve population size estimates for highly mobile species with dynamic spatial distributions. Here, using simulated datasets representative of a large whale population, we demonstrate that incorporating individual mark-recapture data can increase the accuracy and precision of trend estimation and the power to distinguish whether apparent changes in abundance reflect changes in population trend or distribution shifts. We recommend that similar simulation studies are conducted for specific cetacean populations to assess the potential for detecting changes in population dynamics given available data. This approach is especially important wherever population change may be confounded with long-term change in distribution patterns associated with regime shifts or climate change.
Two-stage battery recharge scheduling and vehicle-charger assignment policy for dynamic electric dial-a-ride services
Authors: Ma Tai-Yu
Published: May 2021

Coordinating the charging scheduling of electric vehicles for dynamic dial-a-ride services is challenging considering charging queuing delays and stochastic customer demand. We propose a new two-stage solution approach to handle dynamic vehicle charging scheduling to minimize the costs of daily charging operations of the fleet. The approach comprises two components: daily vehicle charging scheduling and online vehicle–charger assignment. A new battery replenishment model is proposed to obtain the vehicle charging schedules by minimizing the costs of vehicle daily charging operations while satisfying vehicle driving needs to serve customers. In the second stage, an online vehicle–charger assignment model is developed to minimize the total vehicle idle time for charges by considering queuing delays at the level of chargers. An efficient Lagrangian relaxation algorithm is proposed to solve the large-scale vehicle-charger assignment problem with small optimality gaps. The approach is applied to a realistic dynamic dial-a-ride service case study in Luxembourg and compared with the nearest charging station charging policy and first-come-first-served minimum charging delay policy under different charging infrastructure scenarios. Our computational results show that the approach can achieve significant savings for the operator in terms of charging waiting times (–74.9%), charging times (–38.6%), and charged energy costs (–27.4%). A sensitivity analysis is conducted to evaluate the impact of the different model parameters, showing the scalability and robustness of the approach in a stochastic environment.
National trends in emergency department closures, mergers, and utilization, 2005-2015
Authors: Venkatesh Arjun K. , Janke Alexander , Rothenberg Craig , Chan Edwin , Becher Robert D.
Published: May 2021

Study objectives

To describe nationwide hospital-based emergency department (ED) closures and mergers, as well as the utilization of emergency departments and inpatient beds, over time and across varying geographic areas in the United States.

Methods

Observational analysis of the American Hospital Association (AHA) Annual Survey from 2005 to 2015. Primary outcomes were hospital-based ED closure and merger. Secondary outcomes were yearly ED visits per hospital-based ED and yearly hospital admissions per hospital bed.

Results

The total number of hospital-based EDs decreased from 4,500 in 2005 to 4,460 in 2015, with 200 closures, 138 mergers, and 160 new hospital-based EDs. While yearly ED visits per hospital-based ED exhibited a 28.6% relative increase (from 25,083 to 32,248), yearly hospital admissions per hospital bed had a 3.3% relative increase (from 45.4 to 43.9) from 2005 to 2015. The number of hospital admissions and hospital beds did not change significantly in urban areas and declined in rural areas. ED visits grew more uniformly across urban and rural areas.

Conclusions

The number of hospital-based ED closures is small when accounting for mergers, but occurs as many more patients are presenting to a stable number of EDs in larger health systems, though rural areas may differentially affected. EDs were managing accelerating patient volumes alongside stagnant inpatient bed capacity.
Mobilising social support to improve mental health for children and adolescents: A systematic review using principles of realist synthesis
Authors: Bauer Annette , Stevens Madeleine , Purtscheller Daniel , Knapp Martin , Fonagy Peter , Evans-Lacko Sara , Paul Jean
Published: May 2021

Social support is a well-recognised protective factor for children’s mental health. Whilst many interventions exist that seek to mobilise social support to improve children’s mental health, not much is known about how to best do this. We sought to generate knowledge about the ways in which social support can be mobilised to improve children’s mental health. We conducted a systematic review, which followed the principles of a realist synthesis. The following databases were searched: PubMed, CINAHL, Ovid MEDLINE, PsychINFO, EMBASE, Child and Adolescent Studies, EconLit and SocINDEX. Studies were included if the age of participants was between 0 and 18 years and they evaluated or described programme theories of interventions that sought to improve children’s mental health by mobilising social support. Relevance and quality of studies were assessed, and data were extracted and analysed narratively. Thirty-three articles were included. Studies varied substantially with regard to the detail in which they described the processes of mobilising social support and expected mechanisms to improve children’s mental health. Those that provided this detail showed the following: Intervention components included explaining the benefits of social support and relationships to families and modelling friendly relationships to improve social skills. Pathways to improved outcomes reflected bi-directional and dynamic relationships between social support and mental health, and complex and long-term processes of establishing relationship qualities such as trust and reciprocity. Parents’ ability to mobilise social support for themselves and on behalf of children was assumed to impact on their children’s mental health, and (future) ability to mobilise social support. Although interventions were considered affordable, some required substantial human and financial resources from existing systems. Mobilising social support for vulnerable children can be a complex process that requires careful planning, and theory-informed evaluations can have an important role in increasing knowledge about how to best address social support and loneliness in children.
Is domestic agricultural production sufficient to meet national food nutrient needs in Brazil?
Authors: Pompeu João , Nolasco Camille L. , West Paul , Smith Pete , Gerage Jacqueline , Ometto Jean
Published: May 2021

Reducing the impacts of agriculture on the environment is one of the greatest challenges of this century. In Brazil, it is often argued that more land use change is needed to achieve food security. However, analyses seeking to understand the dynamics between agricultural production for exports and food intended for the Brazilian population have not approached the question if national agriculture is sufficient to provide Brazilians with the necessary nutrients, according to nutritional recommendations. In this sense, we sought to combine supply and dietary requirements for food (calories and nutrients) to assess trends in nutrient production and how future population projections and possible changes in diets would affect land necessity for nutritional security. We use sub-national data on agricultural production, population, Food Balance Sheets from FAO, and a compilation of nutritional information on the Brazilian agricultural production. Our results show that, in the last three decades, Brazil produced enough food calories to feed on average 115% of its population. We found that the agricultural land in 2017, without any expansion, is sufficient to feed, at least, 105% of projected population in 2060, considering the same productivity and dietary patterns. In a vegan diet scenario, less than 10% of the land dedicated to agricultural production in the past 30 years would be required. Despite limitations on supplying certain micro-nutrients, a vegan diet would require even less land in the future. We conclude that Brazilian agriculture could deliver enough food to meet Brazilians’ nutritional needs without further land expansion. Food production is compatible with environmental conservation in Brazil, especially if meat consumption is reduced.
Maternal precarity and HPA axis functioning shape infant gut microbiota and HPA axis development in humans
Authors: Jahnke Johanna R. , Roach Jeffrey , Azcarate-Peril M. Andrea , Thompson Amanda L.
Published: May 2021

Background

Early life exposure to adverse environments, and maternal stress in particular, has been shown to increase risk for metabolic diseases and neurobehavioral disorders. While many studies have examined the hypothalamic-pituitary-adrenal axis (HPA axis) as the primary mechanism behind these relationships, emerging research on the brain-gut axis suggests that the microbiome may play a role. In this study, we tested the relationships among maternal precarity and HPA axis dysregulation during the peripartum period, infant gut microbiome composition, and infant HPA axis functioning.

Methods

Data come from 25 mother-infant dyads in the Galápagos, Ecuador. Women completed surveys on precarity measures (food insecurity, low social support, depression, and stress) and gave salivary cortisol samples during and after pregnancy. Infant salivary cortisol and stool were collected in the postpartum. Statistical significance of differences in microbial diversity and relative abundance were assessed with respect to adjusted linear regression models.

Results

Maternal precarity was associated with lower diversity and higher relative abundance of Enterobacteriaceae and Streptococcaceae and a lower relative abundance of Bifidobacterium and Lachnospiraceae. These patterns of colonization for Enterobacteriaceae and Bifidobacterium mirrored those found in infants with HPA axis dysregulation. Maternal HPA axis dysregulation during pregnancy was also associated with a greater relative abundance of Veillonella.

Conclusions

Overall, exposures to precarity and HPA axis dysregulation were associated with an increase in groups that include potentially pathogenic bacteria, including Enterobacteriaceae, Streptococcaceae, and Veillonella, and a decrease in potentially protective bacteria, including Bifidobacterium and Lachnospiraceae, as well as a decrease in overall diversity.
Pseudoprevotella muciniphila gen. nov., sp. nov., a mucin-degrading bacterium attached to the bovine rumen epithelium
Authors: Na Sang Weon , Chun Byung Hee , Beak Seok-Hyeon , Khan Shehzad Abid , Haque Md. Najmul , Lee Jae Sung , Jeon Che Ok , Lee Sang-Suk , Baik Myunggi
Published: May 2021

A Gram-negative, strictly anaerobic mucin-degrading bacterium, which we designated strain E39T, was isolated from the rumen epithelium of Korean cattle. The cells were non-motile and had a coccus morphology. Growth of strain E39T was observed at 30–45°C (optimum, 39°C), pH 6.5–8.5 (optimum, pH 7.5), and in the presence of 0.0–1.0% (w/v) NaCl (optimum, 0.0–0.5%). Strain E39T contained C16:0, C18:0, C18:1 ω9c, iso-C15:0, and anteiso-C15:0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine, unidentified aminophospholipid, and unidentified lipids. The major respiratory isoprenoid quinones were MK-8 and MK-9. The major fermented end-products of mucin were acetate and succinate. The G+C content of the genomic DNA was 46.4 mol%. Strain E39T was most closely related to Alloprevotella rava 81/4-12T with an 87.3% 16S rRNA gene sequence similarity. On the basis of phenotypic, chemotaxonomic, and molecular properties, strain E39T represents a novel genus of the family Prevotellaceae; as such, the name Pseudoprevotella muciniphila gen. nov., sp. nov. is proposed. A functional annotation of the whole genome sequences of P. muciniphila E39T revealed that this bacterium has a putative mucin-degrading pathway and biosynthetic pathways of extracellular polymeric substances and virulence factors which enable bacteria to adhere to the epithelial cells and avoid the host’s immune responses.
Constitutive activation of CTNNB1 results in a loss of spermatogonial stem cell activity in mice
Authors: Boyer Alexandre , Zhang Xiangfan , Levasseur Adrien , Abou Nader Nour , St-Jean Guillaume , Nagano Makoto C. , Boerboom Derek
Published: May 2021

Spermatogenesis requires that a careful balance be maintained between the self-renewal of spermatogonial stem cells (SSCs) and their commitment to the developmental pathway through which they will differentiate into spermatozoa. Recently, a series of studies employing variousin vivoandin vitromodels have suggested a role of the wingless-related MMTV integration site gene family/beta-catenin (WNT/CTNNB1) pathway in determining the fate of SSCs. However, conflicting data have suggested that CTNNB1 signaling may either promote SSC self-renewal or differentiation. Here, we studied the effects of sustained CTNNB1 signaling in SSCs using theCtnnb1tm1Mmt/+;Ddx4-CreTr/+(ΔCtnnb1) mouse model, in which a stabilized form of CTNNB1 is expressed in all germ cells. ΔCtnnb1mice were found to have reduced testis weights and partial germ cell loss by 4 months of age. Germ cell transplantation assays showed a 49% reduction in total functional SSC numbers in 8 month-old transgenic mice.In vitro,Thy1-positive undifferentiated spermatogonia from ΔCtnnb1mice formed 57% fewer clusters, which was associated with decreased cell proliferation. A reduction in mRNA levels of genes associated with SSC maintenance (Bcl6b,Gfra1,Plzf) and increased levels for markers associated with progenitor and differentiating spermatogonia (Kit,Rarg,Sohlh1) were detected in these cluster cells. Furthermore, RNAseq performed on these clusters revealed a network of more than 900 genes regulated by CTNNB1, indicating that CTNNB1 is an important regulator of spermatogonial fate. Together, our data support the notion that CTNNB1 signaling promotes the transition of SSCs to undifferentiated progenitor spermatogonia at the expense of their self-renewal.
DPP-4 inhibitors may improve the mortality of coronavirus disease 2019: A meta-analysis
Authors: Yang Yan , Cai Zixin , Zhang Jingjing
Published: May 2021

Aims

DPP-4 inhibitors are predicted to exert a protective effect on the progression of coronavirus disease 2019 (COVID-19). We conducted this meta-analysis to investigate this hypothesis.

Methods

Four databases, namely, PubMed, Web of Science, EMBASE and the Cochrane Library, were used to identify studies on DPP-4 and COVID-19. The outcome indicators were the mortality of COVID-19. Funnel plots, Begg’s tests and Egger’s tests were used to assess publication bias.

Results

Four articles were included with a total of 1933 patients with COVID-19 and type 2 diabetes. The use of DPP-4 inhibitors was negatively associated with the risk of mortality (odds ratio (OR) = 0.58 95% confidence interval (CI), 0.34–0.99).

Conclusions

DPP-4 inhibitors may improve the mortality of patients with COVID-19 and type 2 diabetes. As few relevant studies are available, more large-scale studies need to be performed.
Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS
Authors: Kohorn Bruce D. , Greed Bridgid E. , Mouille Gregory , Verger Stéphane , Kohorn Susan L.
Published: May 2021

Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall. Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity. Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1. WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion. Here we find that esmd1 suppresses the WAK2cTAP dwarf and stress response phenotype, including ROS accumulation and gene expression. Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found. Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion. The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
ChromInst: A single cell sequencing technique to accomplish pre-implantation comprehensive chromosomal screening overnight
Authors: Gao Fang-Fang , Chen Li , Bo Shi-Ping , Yao Ya-Xin , Xu Zhong-Li , Ding Qing-Yu , Zhang Peng , Lu Si-Jia , Ren Jun
Published: May 2021

Next Generation Sequencing (NGS) is a powerful tool getting into the field of clinical examination. Its preliminary application in pre-implantation comprehensive chromosomal screening (PCCS) of assisted reproduction (test-tube baby) has shown encouraging outcomes that improves the success rate ofin vitrofertilization. However, the conventional NGS library construction is time consuming. In addition with the whole genome amplification (WGA) procedure in prior, makes the single cell NGS assay hardly be accomplished within an adequately short turnover time in supporting fresh embryo implantation. In this work, we established a concise single cell sequencing protocol, ChromInst, in which the single cell WGA and NGS library construction were integrated into a two-step PCR procedure of ~ 2.5hours reaction time. We then validated the feasibility of ChromInst for overnight PCCS assay by examining 14 voluntary donated embryo biopsy samples in a single sequencing run of Miseq with merely 13M reads production. The good compatibility of ChromInst with the restriction of Illumina sequencing technique along with the good library yield uniformity resulted superior data usage efficiency and reads distribution evenness that ensures precisely distinguish of 6 normal embryos from 8 abnormal one with variable chromosomal aneuploidy. The superior succinctness and effectiveness of this protocol permits its utilization in other time limited single cell NGS applications.
Study on the earth pressure of a foundation pit adjacent to a composite foundation with rigid-flexible and long-short piles
Authors: Guo Yuancheng , Gu Shaochuang , Jin Junwei , Li Mingyu
Published: May 2021

Model tests were performed to investigate the lateral earth pressure acting on the retaining structure adjacent to both natural ground (NG) and composite foundation (CFRLP), which were supported with rigid-flexible and long-short piles. Two testing procedures, namely, applying a load to the foundation and rotating the retaining structure along its toe, were considered. The results indicate that the additional lateral earth pressure acting on the retaining structure adjacent to the CFRLP is less than that of the NG in the depth of the reinforcement area strengthened by flexible piles. Compared with NG, the CFRLP yielded a smaller normalized height of application of the lateral earth pressure, suggesting that the CFRLP blocked the horizontal diffusion of the load and had a strong ability to transfer the surcharge load to the deep soil. When rotating the retaining structure, the lateral earth pressure acting on the upper part of the retaining structure experienced limited reduction once the displacement at the top of the retaining structure was greater than 8 mm, whereas the pressure acting on the lower part of the retaining structure continued to decrease with increasing displacement. In addition, a three-dimensional finite element model (FEM) was used to investigate the influence of the pile parameter and the wall-soil friction angle on the additional lateral earth pressure.