bluuebunny/crossref_metadata_2025 huggingface dataset:
Cognitive Behavioral Therapy Improves Physical Function and Fatigue in Mild and Moderate Chronic Fatigue Syndrome: A Consecutive Randomized Controlled Trial of Standard and Short Interventions
Authors: Gotaas Merethe Eide , Stiles Tore C. , Bjørngaard Johan Håkon , Borchgrevink Petter C. , Fors Egil A.
Published: April 2021

Objective: To study whether standard cognitive behavioral therapy (CBT) and a shorter, interpersonal oriented cognitive behavioral therapy (I-CBT) can improve physical function and fatigue in patients diagnosed with mild to moderate chronic fatigue syndrome (CFS) in a multidisciplinary fatigue clinic.

Design: Consecutively 236 participants 18–62 years old meeting the Centre of Decease Control, CDC 1994 criteria, with a subsample also fulfilling the Canadian criteria for CFS, were randomly allocated to one of three groups. Two intervention groups received either 16 weeks of standard CBT or 8 weeks of I-CBT vs. a waiting-list control group (WLC). Primary outcome was the subscale Physical Function (PF) from SF-36 (0–100). Secondary outcome was amongst others fatigue measured by Chalder Fatigue Questionnaire (CFQ) (0–33). Outcomes were repeatedly measured up to 52 weeks from baseline.

Results: The additional effect relative to baseline at post-intervention for SF-36 physical function was 14.2 (95% CI 7.9–20.4 p < 0.001) points higher for standard CBT and 6.8 (0.5–13.2 p = 0.036) points higher for I-CBT compared with the control group. The additional effect relative to baseline at post-intervention for fatigue was 5.9 (95% CI 0.5–10.5 p = 0.03) points lower for standard CBT compared with the control group but did not differ substantially for I-CBT 4.8 (95% CI −0.4 to 9.9 p = 0.07). The positive change in physical function persisted at 1-year follow-up for both treatment groups, and for standard CBT also in fatigue. The two intervention groups did not differ significantly in self-reported physical function and fatigue at the 1-year follow-up. No serious adverse reactions were recorded in any of the groups during the trial period.

Interpretation: A 16-week standard, individual CBT intervention improves physical function and fatigue in CFS outpatients with mild to moderate disease. A shorter 8-week I-CBT program improves physical function. Both treatments are safe, and the effect persist 1 year after baseline.

Clinical Trial registration:ClinicalTrials.gov, Identifier: NCT00920777, registered June 15, 2009.

REK-project number: 4.2008.2586, registered April 2, 2008. Funding: The Liaison Committee for Education, Research and Innovation in Central Norway.
Abnormal ERPs and Brain Dynamics Mediate Basic Self Disturbance in Schizophrenia: A Review of EEG and MEG Studies
Authors: Hamilton Arthur , Northoff Georg
Published: April 2021

Background: Interest in disordered sense of self in schizophrenia has recently re-emerged in the literature. It has been proposed that there is a basic self disturbance, underlying the diagnostic symptoms of schizophrenia, in which the person's sense of being a bounded individual continuous through time loses stability. This disturbance has been documented phenomenologically and at the level of cognitive tasks. However, the neural correlates of basic self disorder in schizophrenia are poorly understood.

Methods: A search of PubMed was used to identify studies on self and schizophrenia that reported EEG or MEG data.

Results: Thirty-three studies were identified, 32 using EEG and one using MEG. Their operationalizations of the self were divided into six paradigms: self-monitoring for errors, proprioception, self-other integration, self-referential processing, aberrant salience, and source monitoring. Participants with schizophrenia were less accurate on self-referential processing tasks and had slower response times across most studies. Event-related potential amplitudes differed across many early and late components, with reduced N100 suppression in source monitoring paradigms being the most replicated finding. Several studies found differences in one or more frequency band, but no coherent overall finding emerged in this area. Various other measures of brain dynamics also showed differences in single studies. Only some of the study designs were adequate to establish a causal relationship between the self and EEG or MEG measures.

Conclusion: The broad range of changes suggests a global self disturbance at the neuronal level, possibly carried over from the resting state. Further studies that successfully isolate self-related effects are warranted to better understand the temporal-dynamic and spatial-topographic basis of self disorder and its relationship to basic self disturbance on the phenomenological level.
Predictors of Intensive Treatment in Patients With Obsessive-Compulsive Disorder
Authors: du Mortier Johanna A. M. , Remmerswaal Karin C. P. , Batelaan Neeltje M. , Visser Henny A. D. , Twisk Jos W. R. , van Oppen Patricia , van Balkom Anton J. L. M.
Published: April 2021

Background: Few studies have investigated which patients with obsessive-compulsive disorder (OCD) do not recover through regular cognitive behavior therapy or pharmacotherapy and subsequently end up in intensive treatment like day treatment or inpatient treatment. Knowing the predictors of intensive treatment in these patients is significant because it could prevent intensive treatment. This study has identified predictors of intensive treatment in patients with OCD.

Methods: Using 6-year longitudinal data of the Netherlands Obsessive Compulsive Disorder Association (NOCDA), potential predictors of intensive treatment were assessed in patients with OCD (n = 419). Intensive treatment was assessed using the Treatment Inventory Costs in Patients with Psychiatric Disorders (TIC-P). Examined potential predictors were: sociodemographics, and clinical and psychosocial characteristics. Logistic Generalized Estimating Equations was used to estimate to what extent the various characteristics (at baseline, 2- and 4-year assessment) predicted intensive treatment in the following 2 years, averaged over the three assessment periods.

Results: Being single, more severe comorbid depression, use of psychotropic medication, and a low quality of life predicted intensive treatment in the following 2 years.

Conclusions: Therapists should be aware that patients with OCD who are single, who have more severe comorbid depression, who use psychotropic medication, and who have a low quality of life or a drop in quality of life are at risk for intensive treatment. Intensive treatment might be prevented by focusing regular treatment not only on OCD symptoms but also on comorbid depression and on quality of life. Intensive treatment might be improved by providing extra support in treatment or by adjusting treatment to impairments due to comorbid depressive symptoms or a low quality of life.
Factors in the Use of Workplace Health Promotion on Back Health. Results of the Survey “German Health Update”
Authors: Hermann Sophie , Starker Anne , Geene Raimund , Jordan Susanne
Published: April 2021

Background: The influence of the working environment on the back health of employees is well-documented. Many companies have begun to offer employees access to services to promote back health. Factors affecting the use of these offers at the population level have received little investigation to date. The current study examined the socio-demographic factors, physical activity and health-related factors, and work-related factors associated with the use of offers of workplace health promotion for back health in Germany.

Materials and Methods: In the representative population-based cross-sectional survey “German Health Update” (GEDA 2014/2015-EHIS) conducted by the Robert Koch Institute, 12,072 employees aged 18–64 years old were surveyed from November 2014 to July 2015 regarding the use of back health services in their companies. In addition to socio-demographic factors, the survey examined working hours, physical activity in leisure time, health awareness, and subjective complaints in the lower back or other chronic back problems in the last 12 months. The interaction of these factors with the utilization of back health services was tested using multiple logistic regression models.

Results: Women used back health services more often than men (women: 25.5%; men: 18.1%). Female gender was associated with part-time employment (OR 0.72) and a strong to very strong level of health awareness (OR 1.40). Male gender was associated with age between 30 and 44 years (OR 1.99) and 45–64 years (OR 2.02), low socioeconomic status (OR 0.48), endurance activity of <2.5 h per week (OR 0.62), and absence of lower back pain or other chronic back conditions for the last 12 months (OR 0.48).

Conclusion: The present study is the first to provide findings regarding the factors associated with the utilization of workplace health promotion to promote back health at the population level, and from the perspective of employees in Germany. The results revealed that the relevant factors for participating in offers differ for women and men. To reach more employees, workplace health promotion offers for back health should be designed specifically for each individual, considering gender and age, working hours, health awareness and behavior, and health state.
Fatty Liver Disease Prediction Model Based on Big Data of Electronic Physical Examination Records
Authors: Zhao Mingqi , Song Changjun , Luo Tao , Huang Tianyue , Lin Shiming
Published: April 2021

Fatty liver disease (FLD) is a common liver disease, which poses a great threat to people's health, but there is still no optimal method that can be used on a large-scale screening. This research is based on machine learning algorithms, using electronic physical examination records in the health database as data support, to a predictive model for FLD. The model has shown good predictive ability on the test set, with its AUC reaching 0.89. Since there are a large number of electronic physical examination records in most of health database, this model might be used as a non-invasive diagnostic tool for FLD for large-scale screening.
Ocean Science Diplomacy can Be a Game Changer to Promote the Access to Marine Technology in Latin America and the Caribbean
Authors: Polejack Andrei , Coelho Luciana Fernandes
Published: April 2021

Ocean science is central in providing evidence for the implementation of the United Nations Law of the Sea Convention. The Convention’s provisions on transfer of marine technology to developing countries aim at strengthening scientific capabilities to promote equitable opportunities for these countries to exercise rights and obligations in managing the marine environment. Decades after the adoption of the Convention, these provisions are under implemented, despite the efforts of international organizations, such as IOC-UNESCO. Latin America and the Caribbean struggle to conduct marine scientific research and seize the opportunities of blue economy due to the limited access to state-of-the-art technology. Ocean science communities in these countries are subject to constraints not foreseeing in international treaties, such as unstable exchange rates, taxation, fees for transportation, costs of maintenance and calibration of technology, challenges to comply with technical standards, and intellectual property rights. Action is needed to overcome these challenges by promoting a closer tie between science and diplomacy. We discuss that this interplay between science and international relations, as we frame science diplomacy, can inform on how to progress in allowing countries in this region to develop relevant research and implement the Convention. We provide concrete examples of this transfer of marine technology and ways forward, in particular in the context of the UN Decade of Ocean Science for Sustainable Development (2021–2030).
Evaluating Virtual Human Role-Players for the Practice and Development of Leadership Skills
Authors: Suárez Gonzalo , Jung Sungchul , Lindeman Robert W.
Published: April 2021

This article reports on a study to evaluate the effectiveness of virtual human (VH) role-players as leadership training tools within two computer-generated environments, virtual reality (VR) and mixed reality (MR), compared to a traditional training method, real human (RH) role-players in a real-world (RW) environment. We developed an experimental training platform to assess the three conditions: RH role-players in RW (RH-RW), VH role-players in VR (VH-VR), and VH role-players in MR (VH-MR), during two practice-type opportunities, namely pre-session and post-session. We conducted a user study where 30 participants played the role of leaders in interacting with either RHs or VHs before and after receiving a leadership training session. We then investigated (1) if VH role-players were as effective as RH role-players during pre- and post-sessions, and (2) the impact that the human-type (RH, VH) in conjunction with the environment-type (RW, VR, MR) had on the outcomes. We also collected user reactions and learning data from the overall training experience. The results showed a regular increase in performance from pre- to post-sessions in all three conditions. However, we did not find a significant difference between VHs and RHs. Interestingly, the VH-MR condition had a more significant influence on performance and task engagement compared to the VH-VR and RH-RW conditions. Based on our findings, we conclude that VH role-players can be as effective as RH role-players to support the practice of leadership skills, where VH-MR could be the best method due to its effectiveness.
A Highly Sensitive and Specific Probe-Based Real-Time PCR for the Detection of Avibacterium paragallinarum in Clinical Samples From Poultry
Authors: Kuchipudi Suresh V. , Yon Michele , Surendran Nair Meera , Byukusenge Maurice , Barry Rhiannon M. , Nissly Ruth H. , Williams Jen , Pierre Traci , Mathews Tammy , Walner-Pendleton Eva , Dunn Patricia , Barnhart Denise , Loughrey Sean , Davison Sherrill , Kelly Dona J. , Tewari Deepanker , Jayarao Bhushan M.
Published: April 2021

Avibacterium paragallinarum (historically called Hemophilus paragallinarum) causes infectious coryza (IC), which is an acute respiratory disease of chickens. Recently, outbreaks of IC have been reported in Pennsylvania (PA) in broilers, layer pullets, and laying hens, causing significant respiratory disease and production losses. A tentative diagnosis of IC can be made based on history, clinical signs, and characteristic gross lesions. However, isolation and identification of the organism are required for a definitive diagnosis. Major challenges with the bacteriological diagnosis of A. paragallinarum include that the organism is difficult to isolate, slow-growing, and can only be successfully isolated during the acute stage of infection and secondary bacterial infections are also common. As there were very limited whole genomes of A. paragallinarum in the public databases, we carried out whole-genome sequencing (WGS) of PA isolates and based on the WGS data analysis; we designed a novel probe-based PCR assay targeting a highly conserved sequence in the recN, the DNA repair protein gene of A. paragallinarum. The assay includes an internal control, with a limit of detection (LOD) of 3.93 genomic copies. The PCR efficiency ranged between 90 and 97%, and diagnostic sensitivity of 98.5% compared with conventional gel-based PCR. The test was highly specific, and no cross-reactivity was observed with other species of Avibacterium and a range of other common poultry respiratory viral and bacterial pathogens. Real-time PCR testing on 419 clinical samples from suspected flocks yielded 94 positives and 365 negatives in agreement with diagnostic bacterial culture-based detection. We also compared the recN PCR assay with a previous HPG-2 based real-time PCR assay which showed a PCR efficiency of 79%.
Deletion of the c2515 and c2516 Genes Affects Iron Uptake and Virulence of APEC O1 Strain E516
Authors: Gao Qingqing , Li Xi , Su Senyan , Yang Lei , Gao Song
Published: April 2021

Avian pathogenic Escherichia coli (APEC), widely spread among poultry, is well-known to cause colibacillosis in chickens, which results in significant losses in poultry industry. The ability to uptake iron in the extra-intestinal environment is prerequisite for APEC survival. For adaptation to the low-iron environments, the bacteria have evolved multiple iron acquisition systems to ensure optimal iron uptake. However, many components of these iron acquisition pathways are still not clearly known. An in silico analysis of the genome of a septicemic APEC O1 strain E516 identified two putative iron transport genes homologous to the c2515 and c2516 genes from uropathogenic E. coli CFT073. In this study, we constructed the single and double gene deletion mutants, and studied their biological characteristic and pathogenic traits through in vitro and in vivo assays. Reverse transcriptase PCR (RT-PCR) analyses demonstrated that the mutations destroying the reading frame of the target genes abolished their transcription. Deletion of the single or double genes of c2515 and c2516 in APEC E516 weakened its ability to produce siderophore. Consistently, the mutants exhibited growth defect under iron-depleted conditions and the intracellular iron levels in the mutants were decreased in comparison with that of the wild-type (WT). Cell infection assays showed that the iron uptake defective mutants were more easily eliminated by the macrophage. Inactivation of the c2515 and c2516 genes affected bacterial colonization of chicken tissues, as well as the 50% lethal dose levels compared with the WT strain. Moreover, the expression levels of several iron uptake-related genes were significantly decreased in the double-deletion mutant. In total, the c2515 and c2516 may involve in siderophore-mediated iron uptake and participate in the pathogenesis of APEC O1 strain E516.
Ecological and Socio-Economic Determinants of Livestock Animal Leptospirosis in the Russian Arctic
Authors: Zakharova Olga I. , Korennoy Fedor I. , Iashin Ivan V. , Toropova Nadezhda N. , Gogin Andrey E. , Kolbasov Denis V. , Surkova Galina V. , Malkhazova Svetlana M. , Blokhin Andrei A.
Published: April 2021

Leptospirosis is a re-emerging zoonotic infectious disease caused by pathogenic bacteria of the genus Leptospira. Regional differences in the disease manifestation and the role of ecological factors, specifically in regions with a subarctic and arctic climate, remain poorly understood. We here explored environmental and socio-economic features associated with leptospirosis cases in livestock animals in the Russian Arctic during 2000–2019. Spatial analysis suggested that the locations of the majority of 808 cases were in “boreal” or “polar” climate regions, with “cropland,” “forest,” “shrubland,” or “settlements” land-cover type, with a predominance of “Polar Moist Cropland on Plain” ecosystem. The cases demonstrated seasonality, with peaks in March, June, and August, corresponding to the livestock pasturing practices. We applied the Forest-based Classification and Regression algorithm to explore the relationships between the cumulative leptospirosis incidence per unit area by municipal districts (G-rate) and a number of socio-economic, landscape, and climatic factors. The model demonstrated satisfactory performance in explaining the observed disease distribution (R2 = 0.82, p < 0.01), with human population density, livestock units density, the proportion of crop area, and budgetary investments into agriculture per unit area being the most influential socio-economic variables. Climatic factors demonstrated a significantly weaker influence, with nearly similar contributions of mean yearly precipitation and air temperature and number of days with above-zero temperatures. Using a projected climate by 2100 according to the RCP8.5 scenario, we predict a climate-related rise of expected disease incidence across most of the study area, with an up to 4.4-fold increase in the G-rate. These results demonstrated the predominant influence of the population and agricultural production factors on the observed increase in leptospirosis cases in livestock animals in the Russian Arctic. These findings may contribute to improvement in the regional system of anti-leptospirosis measures and may be used for further studies of livestock leptospirosis epidemiology at a finer scale.
Bio-Inspired Algorithms and Its Applications for Optimization in Fuzzy Clustering
Authors: Valdez Fevrier , Castillo Oscar , Melin Patricia
Published: April 2021

In recent years, new metaheuristic algorithms have been developed taking as reference the inspiration on biological and natural phenomena. This nature-inspired approach for algorithm development has been widely used by many researchers in solving optimization problems. These algorithms have been compared with the traditional ones and have demonstrated to be superior in many complex problems. This paper attempts to describe the algorithms based on nature, which are used in optimizing fuzzy clustering in real-world applications. We briefly describe the optimization methods, the most cited ones, nature-inspired algorithms that have been published in recent years, authors, networks and relationship of the works, etc. We believe the paper can serve as a basis for analysis of the new area of nature and bio-inspired optimization of fuzzy clustering.
Effect of Organic and Chemical Fertilizer Application on Apple Nutrient Content and Orchard Soil Condition
Authors: Kai Takamitsu , Adhikari Dinesh
Published: April 2021

Apple cultivation in Japan typically involves the use of chemical fertilizers and pesticides which can damage the environment. Therefore, in this study we investigated the orchard soil biochemical characteristics as well as the fruit nutrient contents, and pesticide residues of apples grown either organically (organic fertilizers + reduced pesticides) or with conventional chemical fertilizers and pesticide rates. Compared with conventional chemical fertilizer treatment, the organic fertilizer treatment produced fruit with significantly higher contents of sugar, as well as soil with higher total carbon, total nitrogen, and total phosphorus. There were also significantly greater soil bacterial biomass and N circulation in the organically fertilized treatments. Minimal pesticide residues were detected in the organically fertilized fruits, but in the apples cultivated with conventional rates of fertilizers and pesticides there were significantly higher levels of propargite that was used to control spider mites. These residue levels from the conventionally fertilized orchards exceeded European and Codex residue standards. These results indicate that environmentally friendly arboricultural soil management practices, such as organic fertilizer and reduced pesticide cultivation can enhance nutrient cycling in soil, reduce the burden on the environment, and promote food safety and security.
Chloride Changes Soil–Plant Water Relations in Potato (Solanum tuberosum L.)
Authors: Koch Mirjam T. , Pawelzik Elke , Kautz Timo
Published: April 2021

There is evidence that chloride (Cl―) can lead to both an improved hydration and water use efficiency in plants due to its osmotic properties. The potato crop is widely assumed to be sensitive to Cl―. This is based on studies which found tuber yield or tuber starch reductions following a Cl― fertilization. However, there are also contradictory reports which could not find any detrimental effect of Cl― fertilization on potato plant development. As potato is inefficient in the use of water, we aimed to test if it is possible to improve the hydration status of potato without reducing tuber yield and dry matter by means of Cl― fertilization. We conducted a pot experiment with four different Cl― doses and investigated soil–plant water relations, biomass, tuber yield and dry matter development. Our findings deliver an indication that the potato crop is much less sensitive to Cl― than previously assumed and, more importantly, that a Cl― supply can indeed improve the potato shoot water status. This happened without impairing tuber yield and dry matter. Since potato is very sensitive to drought stress, we assume that Cl― fertilization is a promising measure to improve the drought resilience of potato.
Inheritance of Antibiosis Resistance to the Dectes Stem Borer, Dectes texanus, in Soybean PI165673
Authors: Aguirre-Rojas Lina M. , Buschman Lawrent L. , McCornack Brian , Schapaugh William T. , Scully Erin D. , Zhu Kun Yan , Trick Harold N. , Smith Charles Michael
Published: April 2021

Dectes texanus larvae girdles the stems of soybean and cultivated sunflowers causing significant yield losses in North America. The soybean Plant Introduction (PI) 165673 exhibits antibiosis resistance to the larval stage. The objectives of this study were: (1) to determine the inheritance of D. texanus resistance in PI165673; (2) evaluate PI165673 antibiosis resistance before 21 d post infestation; (3) evaluate girdling damage in PI16563 at the end of the season. K07-1544/PI165673 F2 and F2:3 populations were tested for resistance to D. texanus in 2011 and 2012, and PI165673 antibiosis resistance and girdling damage were evaluated in 2014. Segregation for resistance to D. texanus and heritability estimates in the F2 and F2:3 populations indicated that resistance was controlled by two genes with dominant and recessive epistasis. Antibiosis evaluations indicated: (1) PI165673 contained lower number of larvae and eggs relative to the number of oviposition punctures at 15 d post infestation; (2) the proportion of first-instar larvae was higher in PI165673 at 15 d post infestation; (3) larvae reach the sixth-instar stage in PI165673. None of the PI165673 plants were girdled at the end of the season. Identification of additional sources of D. texanus resistance is required to impair larval development in the stem.
Exogenous Hemin Confers Cadmium Tolerance by Decreasing Cadmium Accumulation and Modulating Water Status and Matter Accumulation in Maize Seedlings
Authors: Liu Xiaoming , Meng Yao , Wei Shi , Gu Wanrong
Published: April 2021

Cadmium (Cd) contamination harms plant growth and human health. The application of hemin (ferroprotoporphyrin IX) can effectively relieve abiotic stresses in plants. This work investigates the effects of hemin on alleviating Cd toxicity and enhancing Cd tolerance in maize seedlings. In this study, maize seedlings were cultivated in nutrient solutions, with a combination of CdCl2 (464 μmol L−1) and hemin (100 μmol L−1). We measured plant growth status, water status, Cd concentration, and Cd distribution in maize seedlings. The results indicated that Cd stress increased Cd accumulation in plants and inhibited plant growth. However, hemin alleviated the growth inhibition and improved water balance, root morphology, and root vitality under Cd stress. Additionally, hemin increased 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), total phenolic content, and phenylalanine ammonia lyase (PAL) activity to enhance tolerance to Cd stress. Hemin reduced Cd concentration, the translocation factor (TF), and the bioconcentration factor (BCF) in maize seedlings under Cd stress. Furthermore, hemin increased Cd concentrations in the cell wall and the soluble fraction of seedling roots, which helped reduce Cd transport from root to shoot. In summary, exogenous hemin could be used for alleviating adverse impacts on maize seedling induced by Cd stress.
A Soft Clustering Approach to Detect Socio-Ecological Landscape Boundaries Using Bayesian Networks
Authors: Ropero Rosa F. , Maldonado Ana D. , Uusitalo Laura , Salmerón Antonio , Rumí Rafael , Aguilera Pedro A.
Published: April 2021

Detecting socio-ecological boundaries in traditional rural landscapes is very important for the planning and sustainability of these landscapes. Most of the traditional methods to detect ecological boundaries have two major shortcomings: they are unable to include uncertainty, and they often exclude socio-economic information. This paper presents a new approach, based on unsupervised Bayesian network classifiers, to find spatial clusters and their boundaries in socio-ecological systems. As a case study, a Mediterranean cultural landscape was used. As a result, six socio-ecological sectors, following both longitudinal and altitudinal gradients, were identified. In addition, different socio-ecological boundaries were detected using a probability threshold. Thanks to its probabilistic nature, the proposed method allows experts and stakeholders to distinguish between different levels of uncertainty in landscape management. The inherent complexity and heterogeneity of the natural landscape is easily handled by Bayesian networks. Moreover, variables from different sources and characteristics can be simultaneously included. These features confer an advantage over other traditional techniques.
Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells
Authors: Lee Sang-Hee , Lee Seunghyung
Published: April 2021

Intermediate filaments (IFs) maintain cell–cell adhesions and are involved in diverse cellular processes such as cytokinesis, cell migration and the maintenance of cell structure. In this study, we investigated the influence of prostaglandin F2 alpha (PGF2α) on cytokeratin and vimentin IFs, Rho-associated protein kinase (ROCK), and cell-cell adhesion in bovine luteal theca cells (LTCs). The luteal cells were isolated from bovine corpus luteum (CL), and the LTCs were treated with 0, 0.01, 0.1 and 1.0 mM PGF2α. Cytokeratin, vimentin and desmoplakin proteins were disrupted and the ROCK protein was significantly increased in PGF2α-treated LTCs. In addition, cell–cell adhesion was significantly (p < 0.05) decreased in the PGF2α-induced LTCs compared to control group (0 mM PGF2α). In conclusion, PGF2α affected the adhesion of cell to cell via disruption of desmoplakin, cytokeratin and vimentin, additionally increasing ROCK in bovine LTCs. These results may provide a better understanding of the mechanism of bovine CL regression.
Stress during Commercial Hatchery Processing Induces Long-Time Negative Cognitive Judgement Bias in Chickens
Authors: Hedlund Louise , Palazon Tiphaine , Jensen Per
Published: April 2021

Worldwide, billions of laying hen chicks are incubated and processed under highly industrialised circumstances every year, which, as we have previously shown, has long-lasting effects. Here, we measured corticosterone incorporated in down feathers to investigate possible stress during the incubation and showed that commercially incubated chicks did not have elevated levels of feather corticosterone, which implies that the main part of the stress effects from hatchery originates from the perinatal period and the handling immediately post-hatch. Further, we investigated how the early hatchery stress affects the chicks’ cognitive welfare state, i.e., “optimism” and “pessimism”. We exposed commercially hatched chickens to a positive cue, an aversive cue and ambiguous cues. The birds were tested at 1 and 10 w of age and the behaviour was compared with that of non-stressed chicks. Latencies to approach ambiguous cues were longer for the stressed chicks, both at 1 (p = 0.008) and at 10 (p = 0.020) weeks of age, i.e., these showed a more pessimistic-like behaviour. We conclude that the main part of the stress effects in commercially hatched chicks originates in the perinatal period, and further, that these birds show lasting levels of increased pessimism. This strongly indicates a long-time poorer welfare state for the animals.
Evaluation of Three Formulations of Essential Oils in Broiler Chickens under Cyclic Heat Stress
Authors: Ruff Jared , Tellez Guillermo , Forga Aaron J. , Señas-Cuesta Roberto , Vuong Christine N. , Greene Elizabeth S. , Hernandez-Velasco Xochitl , Uribe Álvaro J. , Martínez Blanca C. , Angel-Isaza Jaime A. , Dridi Sami , Maynard Clay J. , Owens Casey M. , Hargis Billy M. , Tellez-Isaias Guillermo
Published: April 2021

The objective of the present research was to assess the dietary supplementation of three formulations of essential oils (EO) in chickens under heat stress (HS). Day-of-hatch Cobb 500 chicks (n = 500) were randomly distributed into four groups: 1. HS control + control diets; 2. HS + control diets supplemented with 37 ppm EO of Lippia origanoides (LO); 3. HS + control diets supplemented with 45 ppm LO + 45 ppm EO of Rosmarinus officinalis (RO) + 300 ppm red beetroot; 4. HS + 45 ppm LO + 45 ppm RO + 300 ppm natural betaine. Chickens that received the EO showed significant (p < 0.05) improvement on BW, BWG, FI, and FCR compared to control HS chickens. Average body core temperature in group 3 and group 4 was significantly (p < 0.05) reduced compared with the HS control group and group 2. Experimental groups showed a significant reduction in FITC-d at 42 days, a significant increase in SOD at both days but a significant reduction of IFN-γ and IgA compared with HS control (p < 0.05). Bone mineralization was significantly improved by EO treatments (p < 0.05). Together these data suggest that supplemental dietary EO may reduce the harmful effects of HS.
Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards
Authors: Olveira José G. , Souto Sandra , Bandín Isabel , Dopazo Carlos P.
Published: April 2021

The nervous necrosis virus (NNV) is a threat to fish aquaculture worldwide, especially in Mediterranean countries. Fast and accurate diagnosis is essential to control it, and viral quantification is required to predict the level of risk of new viral detections in field samples. For both, reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) is used by diagnostic laboratories. In the present study, we developed an RT-qPCR procedure for the diagnosis and simultaneous quantification of NNV isolates from any of the four genotypes. The method proved to be highly sensitive in terms of crude virus titer: 5.56–9.88 TCID50/mL (tissue culture infectious dose per mL), depending on the viral strain, and averaging 8.8 TCID50/mL or 0.08 TCID50/reaction. Other standards also yielded very low detection limits: 16.3 genome copies (cps) of purified virus per mL, 2.36 plasmid cps/mL, 7.86 in vitro synthetized RNA cps/mL, and 3.16 TCID50/mL of virus from infected tissues. The diagnostic parameters evaluated in fish samples were much higher in comparison to cell culture isolation and nested PCR. In addition, the high repeatability and reproducibility of the procedure, as well as the high coefficient of determination (R2) of all the calibration curves with any type of standard tested, ensure the high reliability of the quantification of NNV using this RT-qPCR procedure, regardless of the viral type detected and from the type of standard chosen.
Owner-Reported Pica in Domestic Cats Enrolled onto a Birth Cohort Study
Authors: Kinsman Rachel , Casey Rachel , Murray Jane
Published: April 2021

The prevalence and cooccurrence of pica towards different target materials were investigated using prospective data from three questionnaires completed by owners participating in a longitudinal study of UK pet cats. Pica towards one or more material types was reported in 42.9% (229/534), 32.0% (171/534), and 30.9% (165/534) of cats aged approximately 6, 12, and 18 months, respectively. At all timepoints, it was most common for only one material type to be targeted. Associations between potential explanatory variables and “chronic pica” (pica exhibited at all three timepoints) were also explored. Multivariable logistic regression revealed moving to a new house when the cat was aged approximately 6–12 months, renting rather than owning a home, and living in a household without a dog(s) when the cat was aged 2–4 months increased the odds of chronic pica occurrence. This study provides novel data from a cohort of UK pet cats and it is hoped this will increase the understanding of pica and provide direction for areas for future research.
Silencing Antibiotic Resistance with Antisense Oligonucleotides
Authors: Jani Saumya , Ramirez Maria Soledad , Tolmasky Marcelo E.
Published: April 2021

Antisense technologies consist of the utilization of oligonucleotides or oligonucleotide analogs to interfere with undesirable biological processes, commonly through inhibition of expression of selected genes. This field holds a lot of promise for the treatment of a very diverse group of diseases including viral and bacterial infections, genetic disorders, and cancer. To date, drugs approved for utilization in clinics or in clinical trials target diseases other than bacterial infections. Although several groups and companies are working on different strategies, the application of antisense technologies to prokaryotes still lags with respect to those that target other human diseases. In those cases where the focus is on bacterial pathogens, a subset of the research is dedicated to produce antisense compounds that silence or reduce expression of antibiotic resistance genes. Therefore, these compounds will be adjuvants administered with the antibiotic to which they reduce resistance levels. A varied group of oligonucleotide analogs like phosphorothioate or phosphorodiamidate morpholino residues, as well as peptide nucleic acids, locked nucleic acids and bridge nucleic acids, the latter two in gapmer configuration, have been utilized to reduce resistance levels. The major mechanisms of inhibition include eliciting cleavage of the target mRNA by the host’s RNase H or RNase P, and steric hindrance. The different approaches targeting resistance to β-lactams include carbapenems, aminoglycosides, chloramphenicol, macrolides, and fluoroquinolones. The purpose of this short review is to summarize the attempts to develop antisense compounds that inhibit expression of resistance to antibiotics.
Do Orthopedic Surgeons or Neurosurgeons Detect More Hip Disorders in Patients with Hip-Spine Syndrome? A Nationwide Database Study
Authors: Yin Tsung-Cheng , Wegner Adam M. , Lu Meng-Ling , Yang Yao-Hsu , Wang Yao-Chin , Kung Woon-Man , Lo Wei-Cheng
Published: April 2021

Background: Disorders of the hip and lumbar spine can create similar patterns of pain and dysfunction. It is unknown whether all surgeons, regardless of orthopedic or neurosurgery training, investigate and diagnose concurrent hip and spine pathology at the same rate. Methods: Data were retrieved from Taiwan’s National Health Insurance Research Database (NHIRD). Enrolled patients were stratified into hip and spine surgery at the same admission (Both), hip surgery before spine surgery (HS), or spine surgery before hip surgery (SH). The SH group was further subdivided based on whether spine surgery was performed by an orthopedic surgeon (OS) or neurosurgeon (NS), and differences in preoperative radiographic examinations and diagnoses were collected and analyzed. Results: In total, 1824 patients received lumbar spine surgery within 1 year before or after hip replacement surgery. Of these, 103 patients had spine and hip surgery in the same admission (Both), 1290 patients had spine surgery before hip surgery (SH), and 431 patients had hip surgery before spine surgery (HS). In the SH group, patients were categorized into spine surgery by orthopedic surgeons (OS) (n = 679) or neurosurgeons (NS) (n = 522). In the SH group, orthopedic surgeons investigated hip pathology with X-rays more often (52.6% vs. 38.1%, p < 0.001) and diagnosed more cases of hip disease (43.6% vs. 28.9%, p < 0.001) than neurosurgeons. Conclusions: Of patients in Taiwan’s NHIRD who had concurrent surgical degenerative hip and lumbar spine disorders who had spine surgery before hip surgery, orthopedic surgeons obtained hip images and made hip-related diagnoses more frequently than did neurosurgeons.
Use of Neuroanatomic Knowledge and Neuronavigation System for a Safe Anterior Petrosectomy
Authors: Flores-Justa Ana , Luzzi Sabino , Giotta Lucifero Alice , Villalonga Juan F. , Saenz Amparo , Santin-Amo José María , Baldoncini Matias , Campero Alvaro
Published: April 2021

Introduction: The petroclival region is among the most challenging anatomical areas to deal with in skull base surgery. Drilling of the anterior part of the petrous bone during the anterior transpetrosal approach involves the risk of injury of the cochlea, superior semicircular canal, internal carotid artery, and internal auditory canal. A thorough understanding of the microneurosurgical anatomy of this region is mandatory to execute the transpetrosal approaches, decreasing the risk of complications. The aim of this study is to describe the anatomical structures of the petroclival region, highlighting the importance of neuronavigation for safe performance of the anterior transpetrosal approach. Methods: Three adult cadaveric human heads were formalin-fixed and injected with colored silicone. They underwent an axial 1 mm slab CT scan, which was used for neuronavigation during the surgical approaches. The anterior petrosectomy was performed with the aid of neuronavigation during the drilling of the petrous bone. The surgical management of a patient harboring a petroclival meningioma, operated on using an anterior transpetrosal approach, was reported as an illustrative case. Results: The anterior petrosectomy was completed accurately with wide exposure of the surgical target without injuring the cochlea and other structures in all three cadaveric specimens. In the surgical case, no approach-related complications occurred, and a gross total resection of the tumor was achieved. Conclusions: Deep knowledge of the location and relationships of the vital elements located within the temporal bone, along with the use of neuronavigation, are the key aspects to perform the anterior transpetrosal approach safely, reducing the risk of complications.
Ropporin-1 and 1B Are Widely Expressed in Human Melanoma and Evoke Strong Humoral Immune Responses
Authors: Da Gama Duarte Jessica , Woods Katherine , Quigley Luke T. , Deceneux Cyril , Tutuka Candani , Witkowski Tom , Ostrouska Simone , Hudson Chris , Tsao Simon Chang-Hao , Pasam Anupama , Dobrovic Alexander , Blackburn Jonathan M. , Cebon Jonathan , Behren Andreas
Published: April 2021

Antibodies that block immune regulatory checkpoints (programmed cell death 1, PD-1 and cytotoxic T-lymphocyte-associated antigen 4, CTLA-4) to mobilise immunity have shown unprecedented clinical efficacy against cancer, demonstrating the importance of antigen-specific tumour recognition. Despite this, many patients still fail to benefit from these treatments and additional approaches are being sought. These include mechanisms that boost antigen-specific immunity either by vaccination or adoptive transfer of effector cells. Other than neoantigens, epigenetically regulated and shared antigens such as NY-ESO-1 are attractive targets; however, tissue expression is often heterogeneous and weak. Therefore, peptide-specific therapies combining multiple antigens rationally selected to give additive anti-cancer benefits are necessary to achieve optimal outcomes. Here, we show that Ropporin-1 (ROPN1) and 1B (ROPN1B), cancer restricted antigens, are highly expressed and immunogenic, inducing humoral immunity in patients with advanced metastatic melanoma. By multispectral immunohistochemistry, 88.5% of melanoma patients tested (n = 54/61) showed ROPN1B expression in at least 1 of 2/3 tumour cores in tissue microarrays. Antibody responses against ROPN1A and ROPN1B were detected in 71.2% of melanoma patients tested (n = 74/104), with increased reactivity seen with more advanced disease stages. Thus, ROPN1A and ROPN1B may indeed be viable targets for cancer immunotherapy, alone or in combination with other cancer antigens, and could be combined with additional therapies such as immune checkpoint blockade.
ARRB1 Regulates Metabolic Reprogramming to Promote Glycolysis in Stem Cell-Like Bladder Cancer Cells
Authors: Mamouni Kenza , Kim Jeongheun , Lokeshwar Bal L. , Kallifatidis Georgios
Published: April 2021

β-arrestin 1 (ARRB1) is a scaffold protein that regulates signaling downstream of G protein-coupled receptors (GPCRs). In the current work, we investigated the role of ARRB1 in regulating the metabolic preference of cancer stem cell (CSC)-like cells in bladder cancer (BC). We show that ARRB1 is crucial for spheroid formation and tumorigenic potential. Furthermore, we measured mitochondrial respiration, glucose uptake, glycolytic rate, mitochondrial/glycolytic ATP production and fuel oxidation in previously established ARRB1 knock out (KO) cells and corresponding controls. Our results demonstrate that depletion of ARRB1 decreased glycolytic rate and induced metabolic reprogramming towards oxidative phosphorylation. Mechanistically, the depletion of ARRB1 dramatically increased the mitochondrial pyruvate carrier MPC1 protein levels and reduced the glucose transporter GLUT1 protein levels along with glucose uptake. Overexpression of ARRB1 in ARRB1 KO cells reversed the phenotype and resulted in the upregulation of glycolysis. In conclusion, we show that ARRB1 regulates the metabolic preference of BC CSC-like cells and functions as a molecular switch that promotes reprogramming towards glycolysis by negatively regulating MPC1 and positively regulating GLUT1/ glucose uptake. These observations open new therapeutic avenues for targeting the metabolic preferences of cancer stem cell (CSC)-like BC cells.
Validation of the T Descriptor (TNM-8) in T3N0 Non-Small-Cell Lung Cancer Patients; a Bicentric Cohort Analysis with Arguments for Redefinition
Authors: Baum Philip , Taber Samantha , Erdmann Stella , Muley Thomas , Kriegsmann Mark , Christopoulos Petros , Thomas Michael , Winter Hauke , Pfannschmidt Joachim , Eichhorn Martin E.
Published: April 2021

The current pT3N0 category represents a heterogeneous subgroup involving tumor size, separate tumor nodes in one lobe, and locoregional growth pattern. We aim to validate outcomes according to the eighth edition of the TNM staging classification. A total of 281 patients who had undergone curative lung cancer surgery staged with TNM-7 in two German centers were retrospectively analyzed. The subtypes tumor size >7 cm and multiple nodules were grouped as T3a, and the subtypes parietal pleura invasion and mixed were grouped as T3b. We stratified survival by subtype and investigated the relative benefit of adjuvant chemotherapy according to subtype. The 5-year overall survival (OS) rates differed between the different subtypes tumor diameter >7 cm (71.5%), multiple nodules in one lobe (71.0%) (grouped as T3a), parietal pleura invasion (59.%), and mixed subtype (5-year OS 50.3%) (grouped as T3b), respectively. The cohort as a whole did not gain significant OS benefit from adjuvant chemotherapy. In contrast, adjuvant chemotherapy significantly improved OS in the T3b subgroup (logrank p = 0.03). This multicenter cohort analysis of pT3N0 patients identifies a new prognostic mixed subtype. Tumors >7 cm should not be moved to pT4. Patients with T3b tumors have significantly worse survival than patients with T3a tumors.