bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Proximal Tibia as a Donor Site of Cancellous Bone for Intraoral Grafting Procedures—A Retrospective Study
Authors: Malara Piotr , von Krockow Nadine , Niedzielska Iwona , Malara Beata
Published: March 2022

Background: Autogenous bone grafts remain the “gold standard” in maxillofacial reconstructive procedures. The objective of this study was to evaluate the proximal tibia as a donor site of cancellous bone for bone grafting procedures of the mandible on the basis of intraoperative parameters and clinical observations. Methods: The study was based on a medical record search of 40 patients who underwent surgical procedures because of benign pathological lesions of the jaws resulting in 3-wall bone defects of the mandible and qualified for surgical removal of the lesion with simultaneous bone grafting of the defect with autogenous cancellous bone harvested from the proximal tibia. Results: The use of the proximal tibia for bone grafting procedures enables large amounts of cancellous bone (15.09 cc in average) to be obtained. The procedure is characterized by a low risk of early and late complications, which include excessive bleeding, wound infection, lengthy healing time, scars, a loss of sensation around the scars, aching, a dip in bone, swelling and tenderness. Conclusions: The ability to obtain large amounts of cancellous bone and a low risk of intra- and postoperative complications make the proximal tibia an attractive donor site for the bone grafting procedures in maxillofacial surgery.
Activation of the Carboxypeptidase U (CPU, TAFIa, CPB2) System in Patients with SARS-CoV-2 Infection Could Contribute to COVID-19 Hypofibrinolytic State and Disease Severity Prognosis
Authors: Claesen Karen , Sim Yani , Bracke An , De bruyn Michelle , De Hert Emilie , Vliegen Gwendolyn , Hotterbeekx An , Vujkovic Alexandra , van Petersen Lida , De Winter Fien H. R. , Brosius Isabel , Theunissen Caroline , van Ierssel Sabrina , van Frankenhuijsen Maartje , Vlieghe Erika , Vercauteren Koen , Kumar-Singh Samir , De Meester Ingrid , Hendriks Dirk
Published: March 2022

Coronavirus disease 2019 (COVID-19) is a viral lower respiratory tract infection caused by the highly transmissible and pathogenic SARS-CoV-2 (severe acute respiratory-syndrome coronavirus-2). Besides respiratory failure, systemic thromboembolic complications are frequent in COVID-19 patients and suggested to be the result of a dysregulation of the hemostatic balance. Although several markers of coagulation and fibrinolysis have been studied extensively, little is known about the effect of SARS-CoV-2 infection on the potent antifibrinolytic enzyme carboxypeptidase U (CPU). Blood was collected longitudinally from 56 hospitalized COVID-19 patients and 32 healthy controls. Procarboxypeptidase U (proCPU) levels and total active and inactivated CPU (CPU+CPUi) antigen levels were measured. At study inclusion (shortly after hospital admission), proCPU levels were significantly lower and CPU+CPUi antigen levels significantly higher in COVID-19 patients compared to controls. Both proCPU and CPU+CPUi antigen levels showed a subsequent progressive increase in these patients. Hereafter, proCPU levels decreased and patients were, at discharge, comparable to the controls. CPU+CPUi antigen levels at discharge were still higher compared to controls. Baseline CPU+CPUi antigen levels (shortly after hospital admission) correlated with disease severity and the duration of hospitalization. In conclusion, CPU generation with concomitant proCPU consumption during early SARS-CoV-2 infection will (at least partly) contribute to the hypofibrinolytic state observed in COVID-19 patients, thus enlarging their risk for thrombosis. Moreover, given the association between CPU+CPUi antigen levels and both disease severity and duration of hospitalization, this parameter may be a potential biomarker with prognostic value in SARS-CoV-2 infection.
Mental Health in Settings with COVID-19 Positive Cases in the Spanish Population: The Protective Role of the Capacity to Adapt to Change
Authors: Molero Jurado María del Mar , Pérez-Fuentes María del Carmen , Herrera-Peco Iván , Oropesa Ruiz Nieves Fátima , Barragán Martín Ana Belén , Martos Martínez África , Simón Márquez María del Mar , Gázquez Linares José Jesús
Published: March 2022

Background: The capacity to adapt to change in complex, highly demanding situations, such as those derived from the COVID-19 pandemic, is essential in maintaining one’s mental health. This study analyzed the mental health of the Spanish population in settings with COVID-19 positive cases and the protective role of adaptation to change. Methods: The sample consisted of 1160 adult Spaniards aged 18 to 82 with a mean age of 38.29 (SD = 13.71). Data were collected by a CAWI (Computer Aided Web Interviewing) survey which included the General Health Questionnaire (GHQ-28), Adaptation to Change Questionnaire (ADAPTA-10), and an ad hoc questionnaire related to COVID-19 (perceived economic impact, COVID-19 positive diagnosis or not, and whether there was a positive case close to them). Results: The data revealed that the perceived economic impact showed a negative association between the emotional factor and the total score in adaptation to change. Lastly, the emotional factor in adaptation to change operated as a protector from the effect that a setting with COVID-19 positive cases exerts on mental health. Conclusions: Coping with COVID-19 requires strengthening the capacity for adaptation to changes generated in the setting, especially emotional, as it could contribute to improving the mental health of individuals, especially in those settings where they find and know a COVID-19 positive case.
Role of New Anatomy, Biliopancreatic Reflux, and Helicobacter Pylori Status in Postgastrectomy Stump Cancer
Authors: Basso Luigi , Gallo Gaetano , Biacchi Daniele , Carati Maria Vittoria , Cavallaro Giuseppe , Esposito Luca , Giuliani Andrea , Izzo Luciano , Izzo Paolo , Lamazza Antonietta , Polistena Andrea , Tarallo Mariarita , Micarelli Alessandro , Fiori Enrico
Published: March 2022

Distal gastrectomy for benign gastroduodenal peptic disease has become rare, but it still represents a widely adopted procedure for advanced and, in some countries, even for early distal gastric cancer. Survival rates following surgery for gastric malignancy are constantly improving, hence the residual mucosa of the gastric stump is exposed for a prolonged period to biliopancreatic reflux and, possibly, to Helicobacter pylori (HP) infection. Biliopancreatic reflux and HP infection are considered responsible for gastritis and metachronous carcinoma in the gastric stump after oncologic surgery. For gastrectomy patients, in addition to eradication treatment for cases that are already HP positive, endoscopic surveillance should also be recommended, for prompt surveillance and detection in the residual mucosa of any metaplastic-atrophic-dysplastic features following surgery.
Special Issue “Recommendations for Clinical Management of Glaucoma”
Authors: Fogagnolo Paolo
Published: March 2022

Glaucoma is a group of eye conditions that damage the optic nerve head and affect visual function, potentially leading to blindness [...]
Comprehensive Characterization of Human Lung Large Cell Carcinoma Identifies Transcriptomic Signatures with Potential Implications in Response to Immunotherapy
Authors: Ramos-Paradas Javier , Gómez-Sánchez David , Rosado Aranzazu , Ucero Alvaro C. , Ferrer Irene , García-Luján Ricardo , Zugazagoitia Jon , Carrizo Nuria , Enguita Ana B. , Conde Esther , Garrido-Martin Eva M. , Paz-Ares Luis
Published: March 2022

Lung cancer is the leading cause of cancer mortality worldwide, with non-small cell lung cancer (NSCLC) being the most prevalent histology. While immunotherapy with checkpoint inhibitors has shown outstanding results in NSCLC, the precise identification of responders remains a major challenge. Most studies attempting to overcome this handicap have focused on adenocarcinomas or squamous cell carcinomas. Among NSCLC subtypes, the molecular and immune characteristics of lung large cell carcinoma (LCC), which represents 10% of NSCLC cases, are not well defined. We hypothesized that specific molecular aberrations may impact the immune microenvironment in LCC and, consequently, the response to immunotherapy. To that end, it is particularly relevant to thoroughly describe the molecular genotype–immunophenotype association in LCC–to identify robust predictive biomarkers and improve potential benefits from immunotherapy. We established a cohort of 18 early-stage, clinically annotated, LCC cases. Their molecular and immune features were comprehensively characterized by genomic and immune-targeted sequencing panels along with immunohistochemistry of immune cell populations. Unbiased clustering defined two novel subgroups of LCC. Pro-immunogenic tumors accumulated certain molecular alterations, showed higher immune infiltration and upregulated genes involved in potentiating immune responses when compared to pro-tumorigenic samples, which favored tumoral progression. This classification identified a set of biomarkers that could potentially predict response to immunotherapy. These results could improve patient selection and expand potential benefits from immunotherapy.
Retinal Vessel Density in Age-Related Macular Degeneration Patients with Geographic Atrophy
Authors: Hong Suji , Choi Mihyun , Yun Cheolmin , Kim Seong-Woo
Published: March 2022

We compared the retinal vessel density and inner retinal thickness in patients who had one eye with geographic atrophy (GA) and a fellow eye with intermediate age-related macular degeneration (iAMD). The vessel density from the superficial vascular complex (SVC) and deep vascular complex (DVC) through optical coherence tomography angiography and the thickness of the nerve fiber layer, ganglion cell–inner plexiform layer (GCIPL), inner nuclear layer (INL), outer nuclear layer (ONL) on a structural optical coherence tomography thickness map were measured in 28 eyes of 14 GA patients with iAMD in the fellow eye. GA eyes had significantly lower vessel density in the SVC (26.2 ± 3.9% vs. 28.3 ± 4.4%; p = 0.015) and DVC (24.2 ± 2.6% vs. 26.8 ± 1.9%; p = 0.003) than fellow eyes (iAMD). GCIPL and ONL were significantly thinner in GA eyes than in the fellow eyes (p = 0.032 and 0.024 in the foveal areas, p = 0.029 and 0.065 in the parafovea areas, respectively). Twenty-four eyes of 12 patients were followed up for 2 years and seven of the fellow eyes (58.3%) developed GA during the follow-up period and showed reduced vessel density in the SVC (26.4 ± 3.0% vs. 23.8 ± 2.9%; p = 0.087) and DVC (25.8 ± 2.2% vs. 22.4 ± 4.4%; p = 0.047) compared to baseline. Vessel density and GCIPL thickness map measurements are potential GA markers in non-neovascular AMD.
Unreliability of Clinical Prediction Rules to Exclude without Echocardiography Infective Endocarditis in Staphylococcus aureus Bacteremia
Authors: Calderón-Parra Jorge , Diego-Yagüe Itziar , Santamarina-Alcantud Beatriz , Mingo-Santos Susana , Mora-Vargas Alberto , Vázquez-Comendador José Manuel , Fernández-Cruz Ana , Muñez-Rubio Elena , Gutiérrez-Villanueva Andrea , Sánchez-Romero Isabel , Ramos-Martínez Antonio
Published: March 2022

Background: It is unclear whether the use of clinical prediction rules is sufficient to rule out infective endocarditis (IE) in patients with Staphylococcus aureus bacteremia (SAB) without an echocardiogram evaluation, either transthoracic (TTE) and/or transesophageal (TEE). Our primary purpose was to test the usefulness of PREDICT, POSITIVE, and VIRSTA scores to rule out IE without echocardiography. Our secondary purpose was to evaluate whether not performing an echocardiogram evaluation is associated with higher mortality. Methods: We conducted a unicentric retrospective cohort including all patients with a first SAB episode from January 2015 to December 2020. IE was defined according to modified Duke criteria. We predefined threshold cutoff points to consider that IE was ruled out by means of the mentioned scores. To assess 30-day mortality, we used a multivariable regression model considering performing an echocardiogram as covariate. Results: Out of 404 patients, IE was diagnosed in 50 (12.4%). Prevalence of IE within patients with negative PREDICT, POSITIVE, and VIRSTA scores was: 3.6% (95% CI 0.1–6.9%), 4.9% (95% CI 2.2–7.7%), and 2.2% (95% CI 0.2–4.3%), respectively. Patients with negative VIRSTA and negative TTE had an IE prevalence of 0.9% (95% CI 0–2.8%). Performing an echocardiogram was independently associated with lower 30-day mortality (OR 0.24 95% CI 0.10–0.54, p = 0.001). Conclusion: PREDICT and POSITIVE scores were not sufficient to rule out IE without TEE. In patients with negative VIRSTA score, it was doubtful if IE could be discarded with a negative TTE. Not performing an echocardiogram was associated with worse outcomes, which might be related to presence of occult IE. Further studies are needed to assess the usefulness of clinical prediction rules in avoiding echocardiographic evaluation in SAB patients.
Enzyme-Linked Immunosorbent Assay: An Adaptable Methodology to Study SARS-CoV-2 Humoral and Cellular Immune Responses
Authors: Portilho Amanda Izeli , Gimenes Lima Gabrielle , De Gaspari Elizabeth
Published: March 2022

The Enzyme-Linked Immunosorbent Assay is a versatile technique, which can be used for several applications. It has enormously contributed to the study of infectious diseases. This review highlights how this methodology supported the science conducted in COVID-19 pandemics, allowing scientists to better understand the immune response against SARS-CoV-2. ELISA can be modified to assess the functionality of antibodies, as avidity and neutralization, respectively by the standardization of avidity-ELISA and surrogate-neutralization methods. Cellular immunity can also be studied using this assay. Products secreted by cells, like proteins and cytokines, can be studied by ELISA or its derivative Enzyme-linked immunospot (ELISpot) assay. ELISA and ELISA-based methods aided the area of immunology against infectious diseases and is still relevant, for example, as a promising approach to study the differences between natural and vaccine-induced immune responses against SARS-CoV-2.
Spontaneous Osseous Fusion after Remodeling Therapy for Chronic Atlantoaxial Rotatory Fixation and Recovery Mechanism of Rotatory Range of Motion of the Cervical Spine
Authors: Kitamura Kazuya , Ishii Ken , Nagoshi Narihito , Chiba Kazuhiro , Matsumoto Morio , Nakamura Masaya , Watanabe Kota
Published: March 2022

We aimed to investigate the risk factors of spontaneous osseous fusion (SOF) of the atlantoaxial joint after closed reduction under general anesthesia followed by halo fixation (remodeling therapy) for chronic atlantoaxial rotatory fixation, and to elucidate the recovery mechanism of the rotatory range of motion (ROM) after halo removal. Twelve patients who underwent remodeling therapy were retrospectively reviewed. Five patients with SOF were categorized as the fusion group and seven patients without SOF as the non-fusion group. Three dimensional CT was used to detect direct osseous contact (DOC) of facet joints before and during halo fixation, while dynamic CT at neutral and maximally rotated head positions was performed to measure rotatory ROM after halo removal. The duration from onset to initial visit was significantly longer (3.2 vs. 5.7 months, p = 0.04), incidence of DOC during halo fixation was higher (0/7 [0%] vs. 4/5 [80%], p = 0.004), and segmental rotatory ROM of Occiput/C1 (Oc/C1) at final follow-up was larger (9.8 vs. 20.1 degrees, p = 0.003) in the fusion group. Long duration from the onset to the initial visit might induce irreversible damage to the articular surface of the affected facet, which was confirmed as DOC during halo fixation and resulted in SOF. Long duration from the onset to the initial visit and DOC during halo fixation could be used to suggest the risk for SOF. Nonetheless, rotatory ROM of Oc/C1 increased to compensate for SOF.
Spermatic Microbiome Characteristics in Infertile Patients: Impact on Sperm Count, Mobility, and Morphology
Authors: Gachet Clémence , Prat Manon , Burucoa Christophe , Grivard Philippe , Pichon Maxime
Published: March 2022

Through sperm alteration, semen microbiota tend to be recognized as a cause of infertility, but due to the limited number of studies focusing on this ecological niche, this hypothesis remains controversial. This study aimed to characterize and compare the bacterial communities of sperm samples from patients undergoing couple infertility treatment at the time of diagnosis. The study was prospective (September 2019 to March 2020), monocentric, and focused on alterations of spermatic parameters: count, motility, and morphology. After the amplification of the 16S rDNA (V1 to V3), libraries (n = 91, including 53 patients with abnormalities) were sequenced using the MiSeq platform (Illumina). After quality control processing using a homemade pipeline (QIIME2 modules), the main genera were: Prevotella, Finegoldia, Pseudomonas, Peptinophilus, Streptococcus, Anaerococcus and Corynebacterium. Restricted diversity was observed in samples from patients with abnormal sperm morphology (α-diversity, p < 0.05), whereas diversity increased in patients with an abnormal sperm count (β-diversity, p < 0.05). The enrichment of the genus Prevotella and Haemophilus was observed in negative sperm culture samples and samples with abnormal counts, respectively (p < 0.05). Microbiota differed in their composition according to sperm parameters. Finally, this work highlights the need for the optimization of the management of couples undergoing infertility treatment, possibly by modulating the genital microbiome.
Intracoronary Application of Super-Saturated Oxygen to Reduce Infarct Size Following Myocardial Infarction
Authors: Schäfer Andreas , Akin Muharrem , Diekmann Johanna , König Tobias
Published: March 2022

Optimal medical therapy for secondary prevention following acute myocardial infarction reduces non-fatal ischaemic events. Intensive antithrombotic or lipid-lowering approaches have failed to significantly lower mortality. In the past, reduction of infarct size in patients undergoing primary percutaneous revascularisation for acute myocardial infarction had been considered as a surrogate outcome marker. However, infarct size measured by magnetic resonance imaging or SPECT is strongly associated with all-cause mortality and hospitalization for heart failure within the first year after an acute myocardial infarction. Intracoronary administration of super-saturated oxygen (SSO2) immediately after revascularisation is an approach that can be used to reduce infarct size and, therefore, improve cardiovascular outcome in patients with acute myocardial infarction. In this article, we describe the modulation of pathophysiology by SSO2, review the existing trial data and present our first impressions with the technique in real clinical practice.
Evaluation of Mechanical and Wear Properties of Al 5059/B4C/Al2O3 Hybrid Metal Matrix Composites
Authors: Pranavi Uppu , Venkateshwar Reddy Pathapalli , Venukumar Sarila , Cheepu Muralimohan
Published: March 2022

There is a developing interest in efficient materials in automobile and aerospace fields that involves the improvement of metal matrix composites (MMCs) with great properties which incorporate higher strength, hardness and stiffness, better wear and destructive resistance along with better thermal properties. This work deals with the evaluation of the mechanical and wear properties of the newly developed hybrid MMC of Al 5059/B4C/Al2O3 produced by stir casting method. The main aim of the work was to evaluate the mechanical properties of various MMCs fabricated with various weight proportions of ceramic particles (B4C and Al2O3). An increase in the tensile strength and the surface hardness was observed with the increase in the ceramic particles but there was a decrease in the percentage of elongation of the specimen. An increase in the ceramic content in the composite samples made the composite sample brittle (composite) from ductile (base metal).
A 0.5 V Sub-Threshold CMOS Current-Controlled Ring Oscillator for IoT and Implantable Devices
Authors: Ballo Andrea , Pennisi Salvatore , Scotti Giuseppe , Venezia Chiara
Published: March 2022

A current-controlled CMOS ring oscillator topology, which exploits the bulk voltages of the inverter stages as control terminals to tune the oscillation frequency, is proposed and analyzed. The solution can be adopted in sub-1 V applications, as it exploits MOSFETS in the subthreshold regime. Oscillators made up of 3, 5, and 7 stages designed in a standard 28-nm technology and supplied by 0.5 V, were simulated. By exploiting a programmable capacitor array, it allows a very large range of oscillation frequencies to be set, from 1 MHz to about 1 GHz, with a limited current consumption. Considering, for example, the five-stage topology, a nominal oscillation frequency of 516 MHz is obtained with an average power dissipation of about 29 µW. The solution provides a tuneable oscillation frequency, which can be adjusted from 360 to 640 MHz by controlling the bias current with a sensitivity of 0.43 MHz/nA.
Baroclinic Effect on Inner-Port Circulation in a Macro-Tidal Estuary: A Case Study of Incheon North Port, Korea
Authors: Jeong Jae-Soon , Woo Seung-Buhm , Lee Han Soo , Gu Bon-Ho , Kim Jong Wook , Song Jin Il
Published: March 2022

This study investigated the flow patterns and affecting factors in the North Port of Incheon, South Korea, to understand the inner-port circulation characteristics by applying an unstructured grid finite volume community ocean model (FVCOM) together with a field survey. The FVCOM considered the tide, river discharge, surface winds, and atmospheric pressure with the highest resolution of 50 m around the port. The model results were validated with current velocity and salinity observations. In the main channel, the difference in salinity between the sea surface and bottom increased during the neap tide due to weakened tidal currents, thus strengthening the stratification. However, at the inner port, strong stratification was observed during spring tide as the near-surface freshwater from upstream of the estuary moved relatively farther south of the port than that during the neap tide. Freshwater flowed into the port during the flood current and was trapped in the semi-closed geometry of the inner port. The horizontal salinity gradient between the trapped fresher water in the port and saltier water in the main channel increased during the flood current. As a result, density-driven circulations associated with near-bed currents towards the port were maintained for more than 3 h after high tide. This result implies that the baroclinic effect, mainly due to the salinity gradient in the North Port, could significantly affect residual circulation at the inner harbor in a macro-tidal environment and the mass transport mechanism, such as sediment transport. In general, such enhanced baroclinic effects due to salinity and tides are not limited to the North Port and can have greater effects on inner-port circulation in other macro-tidal harbors that suffer from severe sedimentation problems.
Study on Compact Pre-Swirl Duct for Slender Aft-Body Crude Oil Carrier
Authors: Kang Jin-Gu , Kim Moon-Chan , Shin Yong-Jin
Published: March 2022

This study is a parametric investigation of the application of a compact type of pre-swirl duct for a slender aft-body 158k crude oil carrier. The International Maritime Organization (IMO) has created the Energy Efficiency Design Index (EEDI), which is an index related to carbon dioxide emissions, to enforce regulations on newly built ships; many solutions have been proposed, including an increase in propulsion efficiency. In the present study, a new type of energy-saving device (pre-swirl duct), the so-called ring stator, is proposed for 158k crude oil carriers, the hull form of which has been developed as a slender after-body full form to reduce the resistance by delaying separation. A conventional pre-swirl duct is no longer effective at the slender aft-body hull forms because of the small radial flow to the propeller. A parametric study was conducted through Computational Fluid Dynamics (CFD) analysis using the commercial program Star-CCM+, and an improvement of approximately 3% in the propulsion efficiency was achieved in the present study. The efficiency gain was validated through a comparison with the experimental results. The developed ring stator may become applicable to container ships through further parametric studies in the near future.
Fungicide Activity of Culture Extract from Kocuria palustris 19C38A1 against Fusarium oxysporum
Authors: Setiawan Andi , Setiawan Fendi , Juliasih Ni Luh Gede Ratna , Widyastuti Widyastuti , Laila Aspita , Setiawan Wawan A. , Djailani Fernandy M. , Mulyono Mulyono , Hendri John , Arai Masayoshi
Published: March 2022

Secondary metabolites of actinomycetes are a potential source of bioactive compounds in the agricultural sector. This study aimed to determine the fungicidal properties of extracts of marine organism-derived actinomycetes. Actinomycetes were isolated from marine organisms using agar media with 1% colloidal chitin in artificial seawater. Then, the isolates were cultured on liquid media with 1% colloidal chitin in artificial seawater under static conditions for 14 days. The culture was extracted, the fungicide properties were evaluated using the microtiter 96-well plate method, and the influence of inhibition was visualized using apotome and SEM. Finally, the active extract was analyzed using LCMSMS. In the present study, 19 actinomycetes were isolated from marine organisms, and the isolates were examined with regard to their antifungal activities. Of these nineteen isolates, the isolate 19C38A1 was picked out from the rest. Hence, it showed significant control towards F. oxysporum. The prospective strain 19C38A1 was determined to be Kocuria palustris 19C38A1. The extract 19C38A1 was shown to cause damage to cell integrity, indicated by the shrinking form, and inhibited germination in the F. oxysporum; subsequently, the chemical characteristics of the compound produced by the potential isolate 19C38A1 indicated the presence of benzimidazole compounds in the active fraction of C38BK2FA. These results indicate that actinomycetes derived from marine organisms near the coast of Oluhuta, Tomini Bay, Gorontalo, related to strain 19C38A1, are not widely known as sources of valuable fungicides. This preliminary information is important, as it can be used as a basis for further development in the search for fungicides derived from marine actinomycetes.
Impact of Frailty on the Relationship between Blood Pressure and Cardiovascular Diseases and Mortality in Young-Old Adults
Authors: Chun Sohyun , Han Kyungdo , Lee Seungwoo , Cho Mi-Hee , Jeong Su-Min , Jung Hee-Won , Son Ki-Young , Shin Dong-Wook , Lee Sang-Chol
Published: March 2022

The optimal blood pressure (BP) target in older people according to frailty status remains uncertain. This article investigates how frailty affects the association between BP and cardiovascular diseases or mortality, specifically in young-old adults. A retrospective cohort was created for 708,964 older adults with a uniform age of 66 years. The association between BP and myocardial infarction (MI), stroke, or mortality was analyzed using Cox proportional hazards models. The Timed Up and Go test (TUG) was used as a measure of physical frailty. Mean follow-up was 6.8 years, detecting 38,963 (5.5%) events. There was a linear association between increasing systolic BP (SBP) or diastolic BP (DBP) and increased risk of incident MI and stroke, compared to the reference BP (SBP, 110–119 mmHg or DBP, 80–89 mmHg). The risk patterns with high BP remained similar in each TUG group (<10, 10–14, or ≥15 s). A similar pattern of increased risks was found in those who took antihypertensive drugs and who did not, however they were more pronounced in those who did not. The findings support the need to achieve the same BP target in young-old adults with or without frailty to lower the risk of MI, stroke, and mortality.
Artificial Intelligence in Cardiovascular Atherosclerosis Imaging
Authors: Zhang Jia , Han Ruijuan , Shao Guo , Lv Bin , Sun Kai
Published: March 2022

At present, artificial intelligence (AI) has already been applied in cardiovascular imaging (e.g., image segmentation, automated measurements, and eventually, automated diagnosis) and it has been propelled to the forefront of cardiovascular medical imaging research. In this review, we presented the current status of artificial intelligence applied to image analysis of coronary atherosclerotic plaques, covering multiple areas from plaque component analysis (e.g., identification of plaque properties, identification of vulnerable plaque, detection of myocardial function, and risk prediction) to risk prediction. Additionally, we discuss the current evidence, strengths, limitations, and future directions for AI in cardiac imaging of atherosclerotic plaques, as well as lessons that can be learned from other areas. The continuous development of computer science and technology may further promote the development of this field.
Middle Eastern Genetic Variation Improves Clinical Annotation of the Human Genome
Authors: Ramaswamy Sathishkumar , Jain Ruchi , El Naofal Maha , Halabi Nour , Yaslam Sawsan , Taylor Alan , Tayoun Ahmad Abou
Published: March 2022

Genetic variation in populations of Middle Eastern origin remains highly underrepresented in most comprehensive genomic databases. This underrepresentation hampers the functional annotation of the human genome and challenges accurate clinical variant interpretation. To highlight the importance of capturing genetic variation in the Middle East, we aggregated whole exome and genome sequencing data from 2116 individuals in the Middle East and established the Middle East Variation (MEV) database. Of the high-impact coding (missense and loss of function) variants in this database, 53% were absent from the most comprehensive Genome Aggregation Database (gnomAD), thus representing a unique Middle Eastern variation dataset which might directly impact clinical variant interpretation. We highlight 39 variants with minor allele frequency >1% in the MEV database that were previously reported as rare disease variants in ClinVar and the Human Gene Mutation Database (HGMD). Furthermore, the MEV database consisted of 281 putative homozygous loss of function (LoF) variants, or complete knockouts, of which 31.7% (89/281) were absent from gnomAD. This set represents either complete knockouts of 83 unique genes in reportedly healthy individuals, with implications regarding disease penetrance and expressivity, or might affect dispensable exons, thus refining the clinical annotation of those regions. Intriguingly, 24 of those genes have several clinically significant variants reported in ClinVar and/or HGMD. Our study shows that genetic variation in the Middle East improves functional annotation and clinical interpretation of the genome and emphasizes the need for expanding sequencing studies in the Middle East and other underrepresented populations.
Effectiveness of a Mobile App in Reducing Therapeutic Turnaround Time and Facilitating Communication between Caregivers in a Pediatric Emergency Department: A Randomized Controlled Pilot Trial
Authors: Ehrler Frederic , Tuor Carlotta , Trompier Remy , Berger Antoine , Ramusi Michael , Rey Robin , Siebert Johan N.
Published: March 2022

For maintaining collaboration and coordination among emergency department (ED) caregivers, it is essential to effectively share patient-centered information. Indirect activities on patients, such as searching for laboratory results and sharing information with scattered colleagues, waste resources to the detriment of patients and staff. Therefore, we conducted a pilot study to evaluate the initial efficacy of a mobile app to facilitate rapid mobile access to central laboratory results and remote interprofessional communication. A total of 10 ED residents and registered nurses were randomized regarding the use of the app versus conventional methods during semi-simulated scenarios in a pediatric ED (PED). The primary outcome was the elapsed time in minutes in each group from the availability of laboratory results to their consideration by participants. The secondary outcome was the elapsed time to find a colleague upon request. Time to consider laboratory results was significantly reduced from 23 min (IQR 10.5–49.0) to 1 min (IQR 0–5.0) with the use of the app compared to conventional methods (92.2% reduction in mean times, p = 0.0079). Time to find a colleague was reduced from 24 min to 1 min (i.e., 93.0% reduction). Dedicated mobile apps have the potential to improve information sharing and remote communication in emergency care.
Responses of the International Bond Markets to COVID-19 Containment Measures
Authors: To Bao Cong Nguyen , Nguyen Tam Van Thien , Nguyen Nham Thi Hong , Ho Hoai Thu
Published: March 2022

Using an international sample during the COVID-19 outbreak, our study gives evidence that COVID-19 containment measures impact volatility in the international bond markets in different ways. We found that the positive effect of increasing new COVID-19 vaccinations markedly mitigates bond market volatility, while non-pharmaceutical government interventions resembling bad news increase volatility in bond markets. Besides this, changes in total COVID-19 cases and total deaths have co-movement and a significant relationship with this volatility. Our results imply that the investors’ responses to the trigger of increased uncertainty seem to differ in a way that depends on bad or good news as a reflection of the possibility of pandemic control and the health of the economy. The mass vaccinations not only signal a lower probability of stringent government responses to the pandemic but also stabilize investors’ behavior and mitigate compliance fears to open a period of safe living with coronavirus. Our findings are still robust when using alternative measures of independent variables and different forecasting models of conditional volatility.
Outliers and Time-Varying Jumps in the Cryptocurrency Markets
Authors: Dutta Anupam , Bouri Elie
Published: March 2022

We examine the presence of outliers and time-varying jumps in the returns of four major cryptocurrencies (Bitcoin, Ethereum, Ripple, Dogecoin, Litecoin), and a broad cryptocurrency index (CCI30). The results indicate that only Bitcoin returns are contaminated with outliers. Time-varying jumps are present in Bitcoin, Litecoin, Ripple, and the cryptocurrency index. Notably, the presence of jumps in Bitcoin is significant after correcting for outliers. The main findings point to a price instability in some major cryptocurrencies and thereby the importance of accounting for large shocks and time-varying jumps in modelling volatility in the debatable cryptocurrency markets.
Application of Social Network Analysis to Visualization and Description of Industrial Clusters: A Case of the Textile Industry
Authors: Sheresheva Marina Y. , Valitova Lilia A. , Sharko Elena R. , Buzulukova Ekaterina V.
Published: March 2022

This paper discusses the issues of industrial cluster analysis. Initially, the authors explore theoretical approaches to understanding the clusters phenomenon and their identification and analysis. Looking at industrial clusters as network structures connected by various forms of interaction between members, such as ownership linkages, transactions, the presence of common counterparts, and participation in arbitration processes, the authors propose visualizing clusters using social network analysis metrics. This approach helps to address one of the main difficulties when contacting the members of industrial clusters for a subsequent survey or in-depth interviewing. The analysis concludes with a discussion of the proposed method as a way to identify cluster members and determine the most significant ones that are the primary nodes of the network. These key members usually possess enough relevant information about the structure, coordination mechanisms, general strategy, and cluster management system. Therefore, it is possible to limit the list of interviewed respondents without a substantial loss in empirical data quality. The case of the textile industry cluster presented in this paper confirms the applicability of social network analysis to the visualization and description of industrial clusters.
The Determinants of Outward Foreign Direct Investment from Latin America and the Caribbean: An Integrated Entropy-Based TOPSIS Multiple Regression Analysis Framework
Authors: Correa da Cunha Henrique , Singh Vikkram , Xie Shengkun
Published: March 2022

Given that home country factors play a major role in the internationalization of emerging market firms, there is an ever-growing debate on how they influence the intensity of outward foreign direct investment (OFDI) from these regions. This study investigates how home country factors affect the OFDI intensity in Latin America and Caribbean (LAC) countries. We use the entropy weight method, which uses the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method and a balanced panel data consisting of 19 countries from 2007 to 2016. The results show a positive association between macroeconomic performance, formal institutions, infrastructure, technology and the OFDI intensity. Furthermore, we find that robust formal institutions, along with the quality of infrastructure and technology, positively moderate the relationship between macroeconomic performance and the OFDI intensity. These findings show that the internationalization of LAC firms is highly dependent on the contextual conditions in their markets.
Evaluation of Landscape Quality in Valencia’s Agricultural Gardens—A Method Adapted to Multifunctional, Territorialized Agrifood Systems (MTAS)
Authors: Mayordomo-Maya Sandra , Hermosilla-Pla Jorge
Published: March 2022

Multifunctional agrifood systems with noteworthy roots in a territory are the result of a historical yet dynamic specialization. They are present in the place’s knowledge, social connections, collective action, the organization of institutions, innovation and capital. These systems are seen in the landscape, which becomes a resource as well as cultural and environmental heritage. In this regard, it is necessary to study the significance of the many aspects of heritage and landscape in agrifood systems to suitably manage and appreciate them as a territorial resource. This study develops and applies a quantitative method with various criteria to enable the landscape values of multifunctional, territorialized agrifood systems (MTAS in Spanish) to be evaluated, paying attention to essential matters such as the process of heritage recognition and the quality of their landscapes. To do so, a method designed by the authors for evaluating landscapes has been adapted to the peculiarities that define such systems, made up of criteria such as representativeness, authenticity, ecological integrity, vulnerability, accessibility and historical, social and symbolic values. The method has been applied to an exemplary Mediterranean landscape, the agricultural gardens of Valencia, which combine features of MTAS in metropolitan and coastal flatland contexts.
The Importance of Scale and the MAUP for Robust Ecosystem Service Evaluations and Landscape Decisions
Authors: Comber Alexis , Harris Paul
Published: March 2022

Spatial data are used in many scientific domains including analyses of Ecosystem Services (ES) and Natural Capital (NC), with results used to inform planning and policy. However, the data spatial scale (or support) has a fundamental impact on analysis outputs and, thus, process understanding and inference. The Modifiable Areal Unit Problem (MAUP) describes the effects of scale on analyses of spatial data and outputs, but it has been ignored in much environmental research, including evaluations of land use with respect to ES and NC. This paper illustrates the MAUP through an ES optimisation problem. The results show that MAUP effects are unpredictable and nonlinear, with discontinuities specific to the spatial properties of the case study. Four key recommendations are as follows: (1) The MAUP should always be tested for in ES evaluations. This is commonly performed in socio-economic analyses. (2) Spatial aggregation scales should be matched to process granularity by identifying the aggregation scale at which processes are considered to be stable (stationary) with respect to variances, covariances, and other moments. (3) Aggregation scales should be evaluated along with the scale of decision making (e.g., agricultural field, farm holding, and catchment). (4) Researchers in ES and related disciplines should up-skill themselves in spatial analysis and core paradigms related to scale to overcome the scale blindness commonly found in much research.
Measuring the Correlation between Human Activity Density and Streetscape Perceptions: An Analysis Based on Baidu Street View Images in Zhengzhou, China
Authors: Tao Yilei , Wang Ying , Wang Xinyu , Tian Guohang , Zhang Shumei
Published: March 2022

Although investigators are using data sources to describe the visual characteristics of streets, few researchers have linked human perceptions of the street environment with human activity density. This study proposes a conceptualized analytical framework that explains the relationship between human activity density and the visual characteristics of the streetscape. The image-segmentation model DeepLabv3+ automatically extracts each pixel’s semantic information and classifies visual elements from 120,012 collected panoramic street view images of Zhengzhou, China, using the entropy weighting method and weighted superposition to calculate the street perception summary score. This deep learning approach can successfully describe the semantics of streets and the connection between population density and street perception. The study provides a new quantitative method for urban planning and the development of high-density cities.
A New Perspective for Urban Development Boundary Delineation Based on the MCR Model and CA-Markov Model
Authors: Yi Siqi , Zhou Yong , Li Qing
Published: March 2022

In order to control the development of urban space, it is important to explore scientific methods to provide a reference for regional territorial space planning. On the basis of the minimum cumulative resistance (MCR) model and the cellular automaton (CA)-Markov model, we constructed a new technical method for delineating urban development boundaries, exploring the temporal and spatial distribution characteristic of land use in Wuhan from 2010 to 2020 through nighttime and remote sensing images, and simulating the urban development boundaries of Wuhan from 2025 to 2035. The results show that: (1) the scales of Wuhan City’s built-up areas in 2010, 2015, and 2020 were 500 km2, 566.13 km2, and 885.11 km2, respectively, and the trends of expansion run to the east and southeast, and (2) on the basis of the MCR model, the urban development boundary scale of Wuhan City in 2025, 2030, and 2035 from the perspective of actual supply will be 903.52 km2, 937.48 km2, and 1021.44 km2, respectively, and based on the CA-Markov model, the urban development boundary scales of Wuhan City in 2025, 2030, and 2035 from the perspective of ideal land demand will be 912.75 km2, 946.40 km2, and 1041.91 km2, respectively. By combining the results of the two methods, we determined areas of 901.62 km2, 944.39 km2, and 1015.36 km2 as the urban development boundaries of Wuhan City in 2025, 2030, and 2035, respectively. According to the principle of supply–demand balance, the urban development boundary delineated by the integration of the MCR model and CA-Markov model, which is in line with the spatial expansion trend of growing cities, could optimize the urban development pattern; solve the contradiction between urban development, farmland protection, and ecological protection; and provide a methodological reference and decision-making basis for planning practice.
Laryngeal Electromyography in the Therapeutic Process of Patients with Vocal Fold Immobility or Dysmobility
Authors: Krasnodębska Paulina , Szkiełkowska Agata , Czarkwiani-Woźniakowska Ludmiła , Miaśkiewicz Beata , Sinkiewicz Anna , Skarżyński Henryk
Published: March 2022

(1) Background: Laryngeal electromyography (LEMG) plays a key role in classifying the severity of nerve damage and determining the prognosis of the nerve recovery. LEMG is primarily a qualitative study, without a standardized approach to interpretation. The development of qualitative and quantitative analysis would situate LEMG in the gold standard of modern neurolaryngologic diagnostics. The aim of this study was to quantitatively evaluate laryngeal electromyography recorded in patients with vocal fold immobility or dysmobility. (2) Methods: The electromyographic material comprised 84 thyroarytenoid muscles recordings of 42 patients. (3) Results: In our study, we observed significant differences between EMG characteristics of healthy and paralyzed VF. Our study showed that recording laryngeal muscle activity during successive phases of breathing provides additional valuable information. We noticed that the frequency and amplitude of motor unit potentials correlates with the return of vocal fold functionality. (4) Conclusions: Laryngeal EMG guides the clinician on the best course of treatment for the patient. It is therefore important to develop an effective methodology and consensus on the quantitative interpretation of the record. Amplitude and frequency parameters are valuable in predicting neural recovery and in the return of vocal fold mobility.
Dosimetric Comparison of Ultra-Hypofractionated and Conventionally Fractionated Radiation Therapy Boosts for Patients with High-Risk Prostate Cancer
Authors: Piotrowski Tomasz , Yartsev Slav , Krawczyk Jaroslaw , Adamczyk Marta , Jodda Agata , Malicki Julian , Milecki Piotr
Published: March 2022

Recent comparison of an ultra-hypofractionated radiotherapy (UF-RT) boost to a conventionally fractionated (CF-RT) option showed similar toxicity and disease control outcomes. An analysis of the treatment plans for these patients is needed for evaluating calculated doses for different organs, treatment beam-on time, and requirements for human and financial resources. Eighty-six plans for UF-RT and 93 plans for CF-RT schemes were evaluated. The biologically equivalent dose, EQD2, summed for the first phase and the boost, was calculated for dose-volume parameters for organs at risk (OARs), as well as for the PTV1. ArcCHECK measurements for the boost plans were used for a comparison of planned and delivered doses. Monitor units and beam-on times were recorded by the Eclipse treatment planning system. Statistical analysis was performed with a significance level of 0.05. Dosimetric parameter values for OARs were well within tolerance for both groups. EQD2 for the PTV1 was on average 84 Gy for UF-RT patients and 76 Gy for CF-RT patients. Gamma passing rate for planned/delivered doses comparison was above 98% for both groups with 3 mm/3% distance to agreement/dose difference criteria. Total monitor units per fraction were 647 ± 94 and 2034 ± 570 for CF-RT and UF-RT, respectively. The total delivery time for boost radiation for the patients in the UF-RT arm was, on average, four times less than the total time for a conventional regimen with statistically equal clinical outcomes for the two arms in this study.