bluuebunny/crossref_metadata_2025 huggingface dataset:
161 Effects of Supplemental Zn Source (sulfate or Bis-glycinate) on Metabolism, Apparent Absorption, and Retention of Zn by Lambs
Authors: Deters Erin L , VanDerWal Allison , VanValin Katherine , Hansen Stephanie L
Published: May 2021

To assess bioavailability of bis-glycinate Zn (Plexomin Zn, Phytobiotics), 36 crossbred wethers (34 ± 2 kg) were sorted by body weight into three groups and stagger started on a Zn deplete diet (18.6 mg Zn/kg dry matter [DM]; 22.5% neutral detergent fiber) for 45 d prior to a 15-d metabolism period (10 d adaptation, 5 d collection). On d 46, lambs were randomly assigned to dietary Zn treatments (4 lambs/treatment/group): no supplemental Zn (CON) or 15 mg supplemental Zn/kg DM as Zn sulfate (ZS) or bis-glycinate Zn (GLY). Blood was collected from all lambs on d 1, 44, 56, and 61. Liver, small intestine, and muscle samples were collected after euthanasia on d 61. Liver and intestinal gene expression was determined via quantitative real-time polymerase chain reaction. Data were analyzed using ProcMixed of SAS (experimental unit = lamb; fixed effects = treatment, group, and breed). After 15 d of Zn supplementation, plasma Zn concentrations were greatest for GLY versus CON or ZS (P ≤ 0.01), but tissue Zn concentrations were unaffected (P ≥ 0.15). Liver MT1 expression was approximately 2-fold greater for GLY versus CON or ZS (P ≤ 0.07). Intestinal ZIP4 expression tended or was lesser for ZS or GLY versus CON (P ≤ 0.07) and ZNT1 expression tended to be lesser for ZS versus CON (P = 0.07). Zinc intake, fecal output, retention, and apparent absorption were greater for ZS or GLY versus CON (P ≤ 0.01). Apparent absorption of Zn was -5.1, 12.8, and 15.0% for CON, ZS, and GLY, respectively. Although Zn apparent absorption did not differ between supplemental Zn sources (P = 0.71), differences in post-absorptive metabolism may be responsible for the observed increase in circulating Zn concentrations and liver MT1 expression in GLY supplemented lambs, suggesting improved bioavailability of GLY relative to ZS.
255 Evaluation of Different Corn Milling Methods for High-moisture and Dry Corn on Nutrient Digestion
Authors: Coulson Caitlin A , Woita Nicole , Spore Tyler , Wilson Hannah , Butterfield Kylie , Erickson Galen E
Published: May 2021

A 2 × 2 factorial digestion study using seven ruminally cannulated steers evaluated the effect of feeding diets containing 70% (dry matter-basis) high-moisture (HMC) or dry corn (DC), processed with either a hammer mill or Automatic Ag Roller Mill (Pender, NE), on nutrient digestion. Feeding HMC decreased the amount of excreted dry matter (DM) and organic matter (OM; P ≤ 0.01) regardless of mill type, but there was a tendency (P ≤ 0.13) for an interaction between corn type and mill type for DM and OM digestibility. There was no difference between either milling treatments fed as HMC (P ≥ 0.69), but the hammer mill DC diet was more digestible than the roller mill DC (P = 0.05). There was no effect on NDF digestibility, but there was a tendency for an interaction between grain type and processing method for ADF digestibility, with the roller mill DC diet having the lowest (P = 0.02) ADF digestibility and no differences (P ≥ 0.15) among the other treatments. As expected, HMC based diets had greater (P < 0.01) starch digestibility compared to DC, but milling method had no effect (P = 0.56). High moisture corn diets had greater (P = 0.01) DE intake (Mcal/kg), and hammer mill DC tended to be greater (P = 0.07) than roller mill DC. There tended (P = 0.07) to be an interaction for minimum pH, with roller mill HMC and hammer mill DC having the lowest average pH, but not different from hammer mill HMC (P ≥ 0.32). There were no differences (P = 0.56) in average pH, but HMC diets had greater variance (P = 0.04) and greater area under pH 5.6 (P = 0.05) compared to DC based diets. Feeding cattle HMC compared to DC increases nutrient digestibility but milling process had little impact.
256 Evaluation of Replacement of Dietary Corn with Corn Bran Plus Condensed Distillers Solubles on Growth Performance and Carcass Trait Responses in Finishing Beef Steers
Authors: Smith Zachary K , Wilken Mallorie
Published: May 2021

This experiment evaluated replacing dietary corn (50:50 blend of dry-rolled and high-moisture) with corn bran plus condensed distillers solubles (CBCDS) on finishing phase growth performance, efficiency of dietary net energy (NE) utilization, comparative NE value, and carcass trait responses in finishing beef steers. This study used 30 pens of 8 steers/pen assigned to one of three treatments in a randomized complete block design (initial BW=401±43.2 kg); pen served as the experimental unit. Treatments included: 1) finishing diet that contained no corn co-product (Control); 2) finishing diet that contained a dry-corn milling bio-refinery product (20% DM basis inclusion) that replaced corn in the diet: CBCDS; 3) finishing diet that contained a wet-corn milling co-product (20% DM basis inclusion) that replaced corn in the diet: wet corn gluten feed (WCGF). For all analyses, an α of 0.05 determined significance and an α of 0.06 to 0.10 was a tendency. No differences were detected (P ≥ 0.58) among treatments for carcass-adjusted final BW (HCW/0.6433), ADG, or G:F. Observed NE for maintenance and gain was not impacted (P ≥ 0.28) by treatment. No appreciable influence for treatment was detected for the ratio of observed to expected dietary NE for maintenance or gain (P ≥ 0.40). Replacement NEm and NEg values (Mcal/45.4 kg) were determined to be 93.5 and 62.3 for CBCDS and 91.5 and 60.5 for WCGF. There was no influence (P ≥ 0.16) of treatment on DP, HCW, REA, RF, USDA Marbling, KPH, EBF, or final BW at 28% EBF. Treatment tended to influence (P = 0.10) YG, where steers fed WCGF had lesser YG than Control; however, CBCDS diet was intermediate, not differing from WCGF or Control. Distribution of USDA Quality and Yield Grade did not differ (P ≥ 0.29) among treatments. Substitution of corn for CBCDS at 20% of dietary DM can occur without detriment to growth performance or carcass traits.
257 Including Sweet Bran in the Diet of Growing Feedlot Steers Increased Body Weight and Influenced Rate of Feed Disappearance
Authors: Heiderscheit Katie J , Beenken Aubree M , Deters Erin L , Hochmuth Katherine , Jackson Trey D , Messersmith Elizabeth M , VanDerWal Allison , VanValin Katherine , Peschel Joshua , Hansen Stephanie L
Published: May 2021

This study assessed the effect of Sweet BranTM (SWBR; 40% DM inclusion; Cargill Corn Milling, Blair, NE) or modified distiller’s grains (MDGS; 25% DM inclusion) in the diet of newly received feedlot steers on feeding behavior and growing period performance. Diets were formulated for similar metabolizable protein and DM. Two hundred sixteen freshly weaned Angus-cross steers (253 kg ± 18.1) were stratified by BW to 36 pens (n = 18 per treatment; 6 steers/pen). Individual BW were recorded on d 0, 28, and 60. Eight pens per treatment were utilized to assess rate of feed disappearance early (day 5 and 6) and late (53 and 54) during growing by weighing feed in bunks every 2 hours post-delivery for 12 consecutive hours. Feed weights were averaged across consecutive days within pen and timepoint. Slopes were calculated between each timepoint to determine rate of feed disappearance. Data were analyzed with Proc Mixed of SAS 9.4; diet was fixed effect and pen was experimental unit. Feed disappearance slopes were analyzed as repeated measures with the repeated effect of time. Body weights did not differ on d 28 (P ≥ 0.29), but d 60 BW and d 0 to 60 ADG were greater for SWBR than MDGS (P < 0.05). Steers fed SWBR had greater DMI throughout the trial (P < 0.05). Feed efficiency (G:F) did not differ throughout the trial (P ≥ 0.31). Rate of feed disappearance on d 5/6 was greater for SWBR between hours 6–8, and greater for MDGS from hours 10–12. On d 53/54, SWBR had increased feeding rate during hours 0–2, while MDGS was increased from hours 8–10. Steers fed SWBR had a greater rate of feed disappearance earlier in the day and had improved DMI and growth than steers fed MDGS.
275 Comparison of Revalor-xh with a revalor-ih/revalor-h/revalor-200 Re-implant Program on Feedlot Cattle
Authors: Gibbons John , Streeter Marshall N , Nuttelman Brandon L , Erickson Galen E , Dicke Bill
Published: May 2021

A commercial feedlot trial evaluated a 3-implant re-implant program using Revalor-IH/Revalor-H/Revalor-200 compared to a single Revalor-XH (200 mg TBA and 20 mg E2 partially coated) implant strategy on feedlot performance and carcass characteristics of calf fed heifers. Heifer calves (n = 1356) were purchased through time and blocked by source and arrival date, assigned randomly to pen within block, and pen assigned randomly to one of the two implant treatments. Heifers who received the 3-implant re-implant treatment were re-implanted on d67 with Revalor-H and on d137 with Revalor-200. Each block of cattle were harvested on the same study day, which averaged 215 days on feed. Performance data were analyzed as a randomized block design, with the blocking factor as source/arrival time being considered random. Implant strategy was analyzed as a fixed effect with pen (n = 20) as the experimental unit. On a live basis, heifers implanted with Revalor-XH had a tendency for a greater gain efficiency (P = 0.06) though there were no significant differences between implant treatments for final body weight, gain or intake (P ≥ 0.15). There were no significant differences between implant treatments for hot carcass weight, gain, or gain efficiency (P ≥ 0.29) on a carcass-adjust basis. Marbling score and 12th rib fat thickness were not impacted by implant treatment (P ≥ 0.80); however, there was an increase in LM area (P = 0.02) for heifers on the 3-implant re-implant treatment compared to the Revalor-XH implanted heifers. There was also a tendency for a greater calculated YG in heifers who received Revalor-XH (P = 0.07). Heifers fed for approximately 215 d with a single Revalor-XH implant performed similarly to heifers fed the same amount of days receiving a 3-implant re-implant strategy using Revalor-IH/H/200 combination.
273 Effect of Feedlot Implant Dose and Terminal Window Timing on Heifer Performance and Carcass Characteristics
Authors: Pyatt Nathan , Vogel Gary , Brown Mike , White Frank , Kayser William , Homm Justin , Herr Cory , Holland Ben , Word Alyssa
Published: May 2021

Feedlot heifers (n = 3,778; initial BW = 310 kg; SD = 28 kg) were utilized in a 3 x 2 factorial arrangement (9 pens/treatment) to investigate increasing implant dose and terminal window (TW) timing on growth and carcass merit. Component with Tylan® implant dose treatments were 1. Initial TE-IH [80 mg trenbolone acetate (TBA), 8 mg estradiol (E2)] and terminal TE-H (140 mg TBA, 14 mg E2; IH/H), 2. Initial TE-IH and terminal TE-200 (200 mg TBA, 20 mg E2; IH/200), or 3. Initial TE-200 and terminal TE-200 (200/200). Heifers were fed 171 d with terminal implant administered 100 or 60 d prior to slaughter; initial implant window was inverse (71 or 111 d) to TW. Data were analyzed as a mixed model with block included as a random effect. No significant dose x TW interactions occurred for growth or carcass characteristics (P ≥ 0.06) therefore, main effects are presented. Terminal window treatments resulted in similar (P > 0.25) live performance. Heifers reimplanted 60 d prior to slaughter had improved (P < 0.05) marbling score and lower YG2 carcasses. Final weight, gain, HCW, yield, and LM area increased (linear; P < 0.05), while marbling score and fat thickness decreased (linear; P < 0.05) with increasing heifer implant dose. Heifers implanted with IH/200 had lower (P < 0.05) DMI compared to IH/H. IH/200 and 200/200 had improved (+2.1%; P < 0.05) efficiency compared to IH/H. HCW for 200/200 was +3.2 and 5.5 kg greater than IH/200 and IH/H, respectively. 200/200 were leaner with +7.4% YG 1&2 and -5.4% YG 4&5, but -8.5% Prime & Choice carcass compared to IH/H. Shortening TW from 100 to 60 d prior to slaughter did not alter growth performance. Increased implant dose in heifers improved gain, efficiency, HCW and yield with some quality grade considerations.
144 The Effect of Pre-farrow Meal Time on Onset of Parturition in Swine
Authors: Obermier Dalton , Uitermarkt Amanda , Frobose Hyatt , Mote Benny E , Eickhoff Megan , Kibler Jenna , Borg Bart
Published: May 2021

Genetic selection for increased litter size has resulted in a concomitant increase in stillborn rates in modern genetic lines. In commercial farms, pre-parturient sows are typically fed during daytime hours with a high percentage of farrowings occurring unassisted overnight. Therefore, the objective of this study was to determine if feeding time can influence the onset of parturition to better align with farm staff hours and increase available assistance. A total of 1,016 sows were used in a commercial farm in Nebraska, USA, to determine the impact of pre-farrow meal time on onset of parturition. Sows were assigned to either control (0700) or alternative (0200) feeding treatments upon entry to farrowing room (~d112 of gestation) and all sows received 2.23 kg daily. Daily feeding time and quantity was controlled with the use of electronic feeders (Gestal SOLO; JYGA Technologies, Inc.). Farrowings had 24 hr monitoring with traits recorded including onset (ON), duration (DUR), piglet interval (PI), and stillborn percentage (SB). The linear model function (lm) in RStudio was used for regression estimates. Alternative sows farrowed earlier (P < 0.05; 1204 vs. 1256 hr) than control-fed sows. Alternative sows also tended to have shorter DUR (P < 0.10; 6.05 vs. 6.19 hr) and narrower PI times (P < 0.10; 22.98 vs. 24.13 min) than control-fed sows. Stillborn percentage decreased (P < 0.05; 8.53 vs. 9.68%) in alternative sows compared to control-fed sows. Combining ON and DUR, we calculated farrowing completion. Alternative fed sows finished parturition earlier (P < 0.05; 1813 vs. 1929 hr) than control fed sows. These results suggest that the alternative strategy can result in earlier onsets, shorter piglet interval and farrowing duration, and a lower stillborn rate when compared to those fed the control strategy and should especially be of interest to farms without labor for 24hr farrowing assistance.
240 Research Model of Colostrum Intake to Study Effect of Colostrum Bioactive Factors on Piglets Development
Authors: Senn Lisa K , Suarez-Trujillo Aridany , Teeple Kelsey , Casey Theresa , Stewart Kara R
Published: May 2021

Swine colostrum intake within the first 24 hours after birth plays a large role in determining survivability, feed efficiency, growth, and fertility. This study tested the effects of feeding three doses of a homogeneous colostrum sample on BW over 24h, RT, immunocrit, and growth through PND7. Three female piglets were selected from eight litters at birth (n = 24 piglets total; n = 3/liter), and bottle-fed 10% (COL10, n = 8), 15% (COL15, n = 8) or 20% (COL20, n = 8) BW colostrum over 24h. Piglet birth weights were similar between treatments (P = 0.838). Piglets were weighed, RT recorded and immunocrit measured at 24h postnatal, then returned to their litter of origin. BW was recorded daily through PND7. At 24h, COL20 had an ADG of 136.3g, COL15 86.3g, and COL10 8.8g, and an average RT of 39.30⁰C, 38.84⁰C and 38.81⁰C respectively. Immunocrit levels varied from 0.028, 0.024 and 0.017 at 24h. SAS MIXED procedure was used to perform statistical analysis. Post-hoc analysis was performed using Tukey’s test. Colostrum dose had an effect on 24h weight gain (P < 0.001), RT (P = 0.007), immunocrit (P = 0.026), and growth through PND7 (P < 0.05). Treatment, day and interaction were determined as main effects of BW and ADG through PND7. Post-hoc analysis showed COL20 had higher ADG (136.3±54.8g), RT (39.30±0.11°C) and immunocrit (0.028±0.010) at 24h than COL10 (ADG 8.8±24.9g, RT 38.81±0.26°C, immunocrit 0.017±0.004). COL15 ADG (86.3±85.2g) differed from COL10 (P < 0.001), and RT (38.84±0.45) differed from COL20 (P = 0.017). There was no statistical difference in ADG between groups through PND7 (P = 0.874). One piglet within each treatment was crushed before PND7. This model permits controlled studies intended to understand the level of 24h colostrum intake on piglet growth and development using standardized, homogenous colostrum doses.
239 Body Weight and Growth in Normal Cyclic and Acyclic Gilts
Authors: Lents Clay A , Nonneman Dan
Published: May 2021

Anestrus, or failure to express estrus during boar exposure, is commonly observed in replacement gilts, and results primarily from either delayed onset of puberty (prepubertal; PP) or cyclic ovulations without behavioral estrus (behavioral anestrus; BA). Gilts born between 2007 and 2018 at USMARC were observed for age at puberty between 160 and 240 days of age. Mature boars were placed in an alleyway pen while a herdsman observed gilts for standing lordosis in response to the back pressure test. Gilts failing to be observed in estrus by 240 days of age were slaughtered (250.4 ± 0.3 days of age) and reproductive tracts recovered to determine if gilts had ovulated. Gilts were defined as PP (n = 606), BA (n = 649), or Peripubertal (n = 118; PP with large preovulatory follicles on the ovary). There were 96 age-matched, cyclic contemporary gilts included as cyclic control gilts. Body weights were recorded at birth, weaning, 8 weeks, and 21 weeks of age with hot carcass weight (HCW) recorded at slaughter. The objective was to retrospectively determine if growth and HCW differed between these groups. Data were analyzed as a mixed ANOVA using group as a fixed effect with sire and farrowing group to which the gilt was born as random effects. Birth weight, weaning weight, ADG at weaning, and weight at 8 weeks did not differ between groups (P > 0.16). The BA gilts had greater growth rate (weight per day of pig age at 21 weeks; P < 0.02) and HCW (P < 0.0001) than PP and Peripubertal gilts, which also had smaller HCW than control gilts. Some cyclic control gilts (7.3%) that displayed estrous behavior had a prepubertal reproductive tract with no ovulatory activity at slaughter. Results indicated that gilts exhibiting delayed puberty grow slower late in development and during boar exposure. Estrus without ovulation in replacement gilts may be more prevalent than assumed. USDA is an equal opportunity provider and employer.
14 Opening the Black Box of Fertility Prediction
Authors: Kerns Karl
Published: May 2021

Analysis of the U.S. swine herd shows variation in pregnancy rate is more attributable to male-factor subfertility than the dam. To date, a limited degree of correlations has been observed between conventional semen analysis parameters and actual fertility after standard quality cutoffs are met. Thus, a clear ability to predict male-factor fertility is lacking. Knowledge of what makes fertilization competent spermatozoa has been long sought after for centuries. It was only in the last half-century that we understood spermatozoa undergo a biological process after ejaculation to acquire the capacity to fertilize. Since then, work has been done to elucidate the molecular pathways involved in sperm capacitation. Recent technological advances in flow cytometry, namely image-based flow cytometry, allows for high-throughput, single-cell phenotyping. Single-cell phenotyping with biomarkers reflecting significant sperm capacitation events, mitochondrial status, cell health, and more, allows multi-million bioimage data sets to be easily attained. These datasets can then be analyzed utilizing machine and deep learning analytic methods and correlated with single sire field fertility data to open the black box of boar fertility prediction. Our findings establish a new paradigm in sperm function and pave the way for accurate fertility prediction in future precision agriculture applications. This work was supported by the National Institute of Food and Agriculture (NIFA), U.S. Department of Agriculture (USDA) award number 2019-67012-29714.
46 Effects of Implants, Clover, and Fescue Variety on Stocker Steers
Authors: Buessing Zachary T , Farney Jaymelynn K
Published: May 2021

Fescue thrives due to a symbiotic relationship with a fungus called endophyte. Endophyte releases ergot toxins leading to metabolic issues, such as vasoconstriction, rough hair coats and poor cattle gains. Developments have occurred to remove or limit the toxins. The objective of this project was to identify management practices while grazing fescue to improve production and toxicity issues. Sixty-four growing steers (283 ± 14 kg BW) were used in a split-plot experiment, where the whole plot was pasture and the split plot was implants. Whole plot treatment was a 4 x 2 factorial with four levels of fescue (High Endophyte, Low Endophyte, Novel, Endophyte Free) and two levels of legume (Legumes or No Legumes). Split plot included four implant levels (No Implant, Synovex One Grass, Revalor-G, Ralgro). Data collected were weights, hair coat scores, hair length, rectal temperature (every 28d) and ultrasound carcass characteristics last day on pasture. Data were analyzed using the Glimmix Procedure of SAS (v. 9.4, Cary, NC). Steers on high endophyte had the lowest ADG, longest hair, and highest temperature as compared to steers on all other fescue types (P < 0.05). The gain differentiation was observed beginning at D56 through the end of the study (P < 0.05). Overall ADG was not impacted by addition of legume nor implant type (P > 0.10). Steers that were not implanted had a longer hair length throughout many measurement dates (P < 0.05). Steers grazing pastures with legume tended (P < 0.10) to have a higher ultrasound measured marbling score and less muscle depth. This study found that the best management strategy for fescue toxicity is to use non-endophyte or non-toxic varieties of fescue pasture. Contrary to previous research, the addition of implants and legumes for this project showed no improvement in cattle gains.
43 Cow Performance and Lameness Are Affected by Housing in Drylots vs Pasture During the Summer
Authors: Hofer Lucas , Myerscough Megan , Chapple Wes , Meteer Travis T , Trennepohl Keela , McCann Joshua C , Shike Daniel W
Published: May 2021

The objective was to compare the performance of cows housed in drylots or on pasture. Spring-calving, Simmental × Angus cow-calf pairs (n = 108; 77 ± 18 days postpartum) were stratified by age, body weight (BW), body condition score (BCS), calving date, and calf sex. Cows were allotted into six groups which were randomly assigned to drylot or pasture. Drylot cows were limit-fed a ration consisting of corn silage, dried distillers grain, corn stalks, cracked corn, and a corn-based supplement to meet protein and energy requirements. Cows on pasture were rotationally grazed with access to free-choice mineral. Cows were artificially inseminated on day 0. Cow BW, BCS, hair coat scores, locomotion scores, and lameness treatments were evaluated throughout the 110-day experiment. Milk production and composition were evaluated on day 56. Data were analyzed using the MIXED and GLIMMIX (binary data) procedures of SAS. Artificial insemination and overall pregnancy rates did not differ (P ≥ 0.79) between groups. Drylot cows were 22 and 51 kg heavier (P ≤ 0.02) than pasture cows on days 83 and 110, respectively. Drylot cow BCS was greater (P = 0.03) on day 110. Hair coat scores were more desirable (P = 0.03) in drylot cows than pasture cows on day 110. Drylot cows had greater (P = 0.04) milk production than pasture cows. Pasture cows had greater (P ≤ 0.03) milk protein content and milk urea nitrogen. Although locomotion scores did not differ (P ≥ 0.45) on days 0 and 34, they were less desirable (P = 0.02) for the drylot cows on day 110. A greater (P = 0.02) percentage of drylot cows (33%) were treated for lameness than pasture cows (7%). Housing cows in drylots increased BW, BCS, and milk production, but resulted in poorer locomotion scores and increased lameness treatments.
52 Ovarian Follicular Profiling of High- and Low-immunocrit Gilts on Postnatal Day 14
Authors: Lynnes Brianna M , Cushman Robert A , Miles Jeremy R , Oliver William T , Bartol Frank F , Bagnell Carol A , Lents Clay A
Published: May 2021

Colostrum intake by neonatal piglets can be measured using the immunoglobulin immunocrit assay (iCrit). Lactocrine effects occur when maternally derived, milk-borne bioactive factors are transferred to the neonatal circulation with consumption of colostrum during nursing and affect development of somatic tissues, which can have long-term consequences in adulthood. Lactocrine deficiency, indicated by low neonatal iCrit, altered uterine gene expression and reduced fecundity in adult, neonatally lactocrine-deficient gilts. Litter size in pigs is dependent on both ovarian and uterine function. It was hypothesized that lactocrine deficiency affects development of ovarian follicles in gilts. The objective was to determine the number of primordial, primary, and secondary follicles in ovaries of gilts with high (12% ± 0.5; n = 10) or low (1.9% ± 0.4; n = 10) iCrit, determined on postnatal day (PND) 1 after birth. Paired high- and low-iCrit gilts were chosen from the same litters (birth weight; 3.1 ± 0.2 lbs). On PND 14, ovaries were collected and histological sections prepared (3 sections per animal; 30–150 µm apart). Ovarian follicles in each section were staged and the number of follicles in each category were quantified and subjected to ANOVA. Total number of ovarian follicles did not differ with iCrit (P = 0.55; 1,370.6 ± 147.8 follicles per section). The proportion of primordial, primary, and secondary follicles was 89.6 ± 1.15%, 7.7 ± 0.87%, 2.7 ± 0.51%, respectively. The number of primordial (P = 0.55), primary (P = 0.64), and secondary (P = 0.93) follicles did not differ with iCrit. Results indicate that ovarian follicular development of neonatal gilts is not sensitive to immunocrit status. Although lactocrine deficiency did not influence the ovarian follicular profile at PND 14, it remains unknown whether lactocrine programming alters ovarian follicular dynamics in neonatally lactocrine-deficient adults. USDA is an equal opportunity provider and employer.
PSIII-5 Delaying Weaning Age of Lambs Grazing Chicory (Cichorium Intybus) Increases Body Weight
Authors: Campbell Braden J , McCutcheon Jefferson S , Fluharty Francis L , Parker Anthony J
Published: May 2021

Two common challenges in forage-based lamb production systems is the restriction of net energy from grass-based pastures and the production losses associated with parasitic infection. The inclusion of nutrient rich forage in the diet and delaying lamb weaning age has shown to improve lamb growth rates in some systems. The objective of this experiment was to investigate the effects of grazing chicory (Cichorium intybus) and delayed weaning on the growth and health of young pasture-raised lambs. We hypothesized that improving the quality of available forage and delaying weaning would increase the body weight and parasite resilience of lambs. A total of 18 cross-bred ewes and 36 cross-bred lambs (25.2 ± 0.5 kg) were stratified by weight, sex, and randomly assigned to one of two weaning treatments: lambs weaned at 60 days of age (weaned) and lambs remained with their dam and weaned at 120 days of age (delayed weaned). Data were analyzed using PROC MIXED in SAS. Lamb body weight and indices of parasitism were measured every 14 days. There was a treatment × day effect for body weight as delayed weaned lambs were heavier on days 28, 42, and 56 compared with the body weight of weaned lambs (P < 0.02). In addition, there was a treatment × day effect for average daily gain (ADG) whereas delayed weaned lambs demonstrated greater ADG on days 14, 28, 42, and 56 compared with the ADG of weaned lambs (P < 0.02). In monitoring lamb health, there was a treatment × day effect as weaned lambs had a greater packed cell volume (PCV) on day 14 compared with the PCV of delayed weaned lambs (P < 0.03). Conversely, on day 56, weaned lambs had a lower PCV compared with the PCV of delayed wean lambs (P < 0.004). Delayed weaning has shown to be beneficial in improving lamb growth; however, in this instance, delayed weaning demonstrated variable responses in supporting lamb health while grazing on improved pastures.
PSII-22 The Effects of Lactobacillus Fermentum on Sow and Litter Performance
Authors: Knapp Josh K , Winkel Shana M , Bartenslager Alison , Trenhaile-Grannemann Melanie D , San Andres Joice V , Nguyen Khang , Wittler J’Nan , Kinstler Sydney , Fernando Samodha , Miller Phillip , Burkey Thomas
Published: May 2021

The use of probiotics may be an effective strategy in sustainable pig production. The objective of this experiment was to examine the effects of Lactobacillus fermentum LfQi6 (LF) on sow and litter performance. First parity, gestating sows (UNL Resource Population Rep x DNA Genetics Duroc; n = 28) were allotted one of two treatment groups. Treatment groups consisted of a control group (CTL) fed a standard gestation and lactation diet for the duration of the study, while the experimental group was fed CTL supplemented with 10^10 CFU LF per day from d 80 of gestation through lactation. Individual piglet body weights (BW) were recorded weekly. LF sows tended to enter the farrowing crates lighter than the control sows (209.93 vs 200.91kg; P = 0.0794). LF sows had a smaller loin eye area when compared to CTL sows (6.24 vs 5.86 cm2; P = 0.0374) when entering the farrowing crates. However, LF sows tended to lose less loin area per day when in the crate (0.025 vs 0.012 cm2/d; P = 0.078); resulting with there being no difference in loin area when the sows left the farrowing crates. Piglet BW tended to be greater at weaning for the sows fed the LF (5.37 vs 5.66 kg, P = 0.0528). The mean piglet BW were 1.25,1.33; 2.37,2.44; 4.08,4.22; and 5.37,5.66 at d 0, 7, 14, 21; respectively for CTL and LF. Sows fed the LF had fewer pigs born alive per litter (16.09 vs 14.12; P < 0.05) when compared to the sows fed the control diet. However, piglet mortality tended to be decreased for LF compared to CTL (3 vs 1.76; P = 0.0645). Results suggest that supplementation with Lactobacillus fermentum LfQi6 may provide some benefits with respect to sow and litter performance.
PSII-19 Effect of a Sensory Additive on Sow Feed Intake During Lactation
Authors: Tsai Tsung Cheng Cheng , Davis Nathan , Bass Benjamin , Tedo Gemma , Morais Sofia , Maxwell Charles V
Published: May 2021

Sow feed intake during lactation is crucial to the maintenance of sow body condition, milk production, and litter performance. Loss of body weight or condition during this time could compromise future reproductive performance. Two groups (n = 52 total) of breeding age gilts and sows were used to evaluate whether a sensory additive would improve intake during the lactation period. Gilts/Sows were weighed, and back fat depth was measured, at 110 d of gestation prior to entering the lactation room, and again at weaning. The sows were sorted by parity and then allotted by BW at d 110 to Control or 0.075% sensory additive (Luctamax® SowVive, Lucta S.A., Barcelona, Spain). Experimental lactation diets were offered upon entry to the lactation room and fed through weaning. During this time feed was weighed back every three days. Gilts/sows were fed 2.72 kg/head/day until farrowing, and sows were placed on full feed 24 hours post-farrowing and allowed to eat ad libitum. Data was analyzed using the PROC MIXED of SAS as an RCBD with treatment as the fixed effect and group as a random effect. During lactation sows on the sensory additive diet showed an increase in intake during days 10 to 21 when compared to sows on the control diet (Table 1). Although it was not significantly different, sows fed sensory additive had numerically heavier weaning BW (239.4 vs 236.2 kg, P = 0.42) and increased litter weight gain (51.96 vs 48.69 kg, P = 0.24) compared to control fed sows. However, backfat thickness change during lactation did not differ between control and sensory additive fed sows (-4.74 vs -5.81 mm, P = 0.65). In conclusion, in the current study lactation diets supplemented with a sensory additive stimulated sow appetite during the lactation period.
PSII-17 Phosphorus Release Values in Growing Pigs Fed Increments of Optiphos Plus Phytase in Pelleted Diets
Authors: Crenshaw Tom D , Haas Caley R , Hansen Megan , Capdeville Mariola Grez , Booth Jeff , Sparks Chris
Published: May 2021

The efficacy of phytase (Pase) from OptiPhos Plus G (Huvepharma, Peachtree City, GA) was compared to inorganic phosphorus (iP) supplements to assess phosphorus equivalency. In 6 replicate 28-d trials, 288 crossbred F2 (Duroc X LR X LW) pigs (20.0 kg, 6 pigs/pen, 48 pens) were randomly assigned to 1 of 8 diets. Pig sex was balanced within pens. Basal corn-soybean meal diets, formulated to exceed nutrient requirements except for Ca and P, supplied 1.30% lysine from the same amounts of all ingredients. Sand was used to balance variable amounts of monocalcium phosphate and limestone. Diets supplied either 0.061, 0.156, or 0.241% iP from monocalcium phosphate or increments of 250, 500, 750, 1,000 and 1,500 FTU/kg from Pase. All diets were pelleted (160°F, 2.5 tons/hr). The modified pellet durability index averaged 92.8% and 88.7% for all diets in each of 2 mixes. Non-linear growth responses to increments of iP and Pase were detected (P < 0.05). Using nonlinear regression models, maximum gain was calculated at 0.23% iP and 1144 FTU/kg Pase (equivalent to 0.31% available P or 0.38% STTD P). Likewise, from non-linear responses (P < 0.05), maximum response in whole-body bone mineral content (WBBMC) from analysis of dual energy x-ray absorptiometry scans were calculated at 1227 FTU/kg Pase. The maximum Pase response for WBBMC was below expectations and raised concerns for potential Ca limitations in diets at higher Pase supplements. Only linear WBBMC responses (P < 0.001) to iP levels were detected. Responses across iP and Pase treatments for femur bone mineral content reflected the same conclusions as responses to WBBMC. As growth responses to iP and Pase diets were non-linear, simple conversions of phytase activity to phosphorus release was not straight-forward. However, non-linear equations can predict economic values of target levels as a function of ingredient costs.
PSII-12 Effects of Available Space in Pens and Homeopathic Product Supplement Consisting of Botanicals and Mineral mixture on the Performance and Production Traits of Finishing Pigs
Authors: Upadhaya Santi Devi , Palanisamy Thanapal , Park Hyun Ju , Kim Inho , Jung Hong Ik
Published: May 2021

A total of 144 mixed sex pigs [(Landrace × Yorkshire) × Duroc] with an initial average body weight (BW) of 52 kg were used in a 11-week trial in a 2 × 2 factorial design with the following factors: space allocations [3pigs/pen vs 5 pigs/pen] and 0 or 0.2% homeopathic product supplementation to evaluate growth performance, nutrient digestibility, meat quality and stress related hormones. The homeopathic product supplementation resulted in an increased (P < 0.05) BW at week 11, average daily gain (ADG) during days 42–63 and 64–77. In addition, an increase (P < 0.05) in ADG and average daily feed intake (ADFI) during overall experiment period and an increase (P < 0.05) in apparent total tract digestibility (ATTD) of dry matter (DM) at day 77 were observed in pigs receiving homeopathic product. A trend in increase (P=0.088) in serum cortisol concentration during day 42 and increase (P > 0.05) during day 77 was observed in pigs with less space allocation. However, supplementing the diet with homeopathic product reduced cortisol concentrations during day 42 (P < 0.05) and day 77 (P = 0.084) respectively suggesting the effectiveness of homeopathic product in reducing the stress caused by less space allocation.
PSII-18 Effect of L-trp Source and Standard Ileal Digestible (SID) Tryptophan to Lysine Ratio on Growth Performance of Nursery Pigs
Authors: Tsai Tsung Cheng Cheng , Bottom kristopher , Coble Kyle , Gerhart Amanda J , Chewning Jeffrey , Maxwell Charles V , Davis Nathan
Published: May 2021

To evaluate the different sources of L-Trp on growth performance, a total of 288 weaned pigs (PIC1050 x DNA600) were stratified by gender and BW, and assigned to 1 of 7 dietary treatments. Treatments were control (devoid of feed grade Trp to create SID Trp/Lys = 17), and L-Trp from (Ajinomoto CO., INC., TrpA vs Daesang CO., LTD., TrpD) was added to form SID Trp:Lys ratios of 19, 21, and 23. Chlortetracycline (Zoetis Inc. Parsippany-Troy Hills, NJ) was supplemented in phase 1 (6 d) &2 (9 d), and Mecadox (Philbro Animal Health Corp., Ridgefield Park, NJ) was added in phase 3 (10 d) while antibiotic free diets were fed in phase 4 (17 d). Individual pig BW and pen feed intake was measured at each phase to determine BW, ADG, ADFI and F:G ratio. Data were analyzed as Mixed procedure of SAS as RCBD. Orthogonal contrast was conducted to determine linear and quadratic response with SID Trp/Lys from 17 (control) to 23 for each source of L-Trp. Adding both sources of L-Trp had minimum impact on growth performance in phase 1–3. As in phase 4, a tendency and significant linear increase on ADG with increasing level of TrpA (P = 0.09) and TrpD (P = 0.04) was observed with the highest ADG in pigs fed TrpA19. Pigs fed increasing level of SID Trp/Lys from TrpA had a quadratic reduction on F:G ratio (P = 0.09). The results suggested that a Trp:Lys ratio over 17 may enhance growth performance and source of L-Trp may impact performance.
PSIII-10 The Effect of Sire Line on Grow-finish Performance, Carcass Characteristics, and Meat Pricing Variables for Commercial Crossbred Pigs
Authors: Manu H , Fletcher M , Fang K , Junior Sudario Roberto Silva , Dunkelberger J , Boler D , Eggert J , Baidoo S K
Published: May 2021

The objective of this study was to evaluate the effect of sire line on wean-to-finish performance, carcass characteristics, and meat pricing variables. Pigs used for this study were the progeny of a commercial Landrace x Large White female mated to one of the following sire lines: a purebred Duroc line (Line S1), or a synthetic (Line S2) or purebred Duroc line (Line S3) originating from a different genetic source. An equivalent number of pigs (n =144) were used per group, for 432 pigs placed on test. Average initial weights per line were 6.67 ± 0.24, 6.67 ± 0.24, and 6.60 ± 0.24 for lines S1, S2, and S3, respectively. At placement, 8 pigs were assigned to each pen (split sex) and blocked by line and initial BW, with 18 replicates per line. Data were analyzed at the pen level using the PROC MIXED procedure of SAS, where the effects of line, sex, and line*sex were fitted as fixed effects and replicate was fitted as a random effect. Overall, piglets from line S2 (0.403 vs 0.381; P ≤ 0.0004) and line S3 (0.402 vs 0.381; P ≤ 0.001) had improved GF compared with piglets from line S1. The ADFI was greater in piglets from line S1 relative to piglets from line S2 (2.264 vs 2.117 kg; P ≤ 0.0001) and line S3 (2.264 vs 2.159 kg; P ≤ 0.004). The ADG, HCW, dressing yield, and loin depth were not different among treatment (P > 1.00). Piglets from line S3 had greater percent lean (57.12 vs 56.29 %; P ≤ 0.0001) and better carcass grade premium ($7.07 vs 6.60; P ≤ 0.0291) relative to piglets from line S1. In conclusion, line S1 piglets had greatest ADFI, but line S2 pigs and line S3 piglets had better feed efficiency. Line S3 pigs were leaner and had better carcass grade premium.
PSIV-8 Effect of Feeding Levucell SB® 10 Titan Advantage and YANG on Performance, Blood Parameters, and Fecal VFA of Nursery Pigs
Authors: Tran Huyen , Shields Michael , Arentson Roger , Weeden Terry , de Rodas Brenda
Published: May 2021

A total of 496 weaned pigs (19 ± 1 d of age) were used in a 3-phase feeding program to evaluate effects of Levucell SB 10 Titan Advantage, a live yeast, Saccharomyces cerevisiae boulardii (LEV; Elanco) and a non-viable yeast product (YANG; Elanco) on performance, complete blood count (CBC), and fecal VFA of nursery pigs. Pigs were blocked by weaning BW and gender and allotted to 44 pens (11–12 pigs/pen) which was assigned to one of 4 dietary treatments (11 pens/treatment). The study was designed as 2 x 2 factorial with LEV and YANG. Pigs were fed to meet or exceed nutrient requirements (NRC, 2012). Blood (d 10 and 22) and fecal samples (d 22) were collected from one pig per pen for CBC and VFA analyses. During d 0–7, feeding LEV or YANG alone improved (P < 0.05) G:F by 20% and 12%, respectively. There was a tendency of LEV*YANG interaction (P < 0.10) where feeding LEV increased (P < 0.05) BW and ADG compared to the control (CON). During d 0–14, feeding YANG with LEV reduced (P < 0.05) BW, ADG, and G:F compared to feeding YANG or LEV alone. Pigs fed LEV had an improved G:F compared to pigs fed CON or YANG with LEV (P < 0.05). During d 21–42, there was a tendency (P < 0.10) for a LEV*YANG effect where pigs fed CON had a lower G:F compared to other treatments. Overall, feeding LEV alone increased (P < 0.01) G:F (2.4%) and numerically increased final BW (0.60 kg) compared to CON. LEV tended to increase blood lymphocytes (P = 0.11) and increased platelets and platelet hematocrit (P < 0.05) compared to CON. There were no differences in fecal VFA measures. The results of this study indicate that feeding LEV increased G:F of nursery pigs.
PSIV-14 Influence of Feed Grade Amino Acid Inclusion Level in Late Nursery and Grower Diets Fed to Pigs from 10 to 35 Kg
Authors: Williams Hadley , Tokach Mike D , Woodworth Jason C , DeRouchey Joel M , Goodband Robert D , Post Zach , Hastad Chad W , Haydon Keith
Published: May 2021

A total of 912 pigs (PIC TR4 × (Fast LW × PIC L02)) were used in a 43-d trial to evaluate the influence of feed grade amino acid levels in late nursery and grower diets containing 30% dried distillers grains with solubles (DDGS) on growth performance. Pigs were randomly assigned to pens (19 pigs per pen) and pens were randomly allotted in weight blocks to 1 of 4 dietary treatments with 12 pens per treatment. Treatment diets were fed in 2 phases from 10 to 19.5 kg and 19.5 to 35 kg with digestible lysine at 1.31 and 1.15%, respectively). Predetermined orthogonal contrasts were used to evaluate linear or quadratic effects based on percentage of digestible lysine from intact protein versus feed grade lysine. Dietary treatments contained low, medium, high, or very high levels of feed grade amino acids with L-lysine added at 0.25, 0.40, 0.55, and 0.70% of the diet, respectively, with all other amino acids added as needed to meet minimum ratios relative to lysine (60% Ile; 58% Met and Cys; 65% Thr; 19% Trp; 72% Val). Overall, from d 0 to 43, there was an increase (quadratic, P< 0.020) in average daily gain (ADG) and average daily feed intake (ADFI) with pigs fed increasing levels of feed grade amino acids having the greatest gain and feed intake at the medium and high inclusion of feed grade amino acids, respectively. For overall gain:feed (G:F), pigs fed the medium level of feed grade amino acids had improved G:F (P=0.002) compared to pigs fed the high and very high feed grade amino acids with the pigs fed the low feed grade amino acids intermediate. In summary, feeding pigs medium levels of feed grade amino acids resulted in increased ADG and G:F during the late nursery and grower period.
PSIV-15 Influence of Particle Size of Enogen Feed Corn and Conventional Yellow Dent Corn on Lactating Sow Performance
Authors: Williams Hadley , Tokach Mike D , Woodworth Jason C , Goodband Robert D , DeRouchey Joel M , Dritz Steve S , Wecker Haley , Calderon Hilda I
Published: May 2021

A total of 107 sows (Line 241; DNA, Columbus, NE) across 4 batch farrowing groups were used to evaluate the effects of corn source and particle size on sow and litter performance. Treatments were arranged in a 2×2 factorial with main effects of corn source (Enogen® Feed corn (Syngenta Seeds, Downers Grove, IL) or conventional yellow dent corn) and ground corn particle size (600 or 900 µm). Sows were blocked by parity and BW upon arrival to the farrowing house. There were approximately 27 sows per treatment, sow was considered the experimental unit, dietary treatment was a fixed effect, and sow group and block were used as random effects. Main effects of corn source and particle size as well as their interactions were tested. From farrowing to weaning, there was a tendency for a source×particle size interaction (P=0.065) for sow BW change. Sows fed 900 µm Enogen Feed corn had decreased BW loss compared to sows fed other treatments which were similar in BW loss. There was a source×particle size interaction (P=0.048) for lactation ADFI with sows fed 900 µm conventional yellow dent corn having lower feed intake than the sows fed 600 µm conventional yellow dent corn, whereas sows fed 900 µm Enogen Feed corn had greater feed intake compared to the sows fed 600 µm Enogen Feed corn. There was a tendency for a particle size main effect (P<0.10) for litter ADG (2,849 vs 2,635 g/d) and total litter gain (45.7 vs 42.3 kg), with sows fed corn ground to 600 µm having increased litter ADG and total litter gain compared to sows fed corn ground to 900 µm. In summary, there were few differences in sow or litter characteristics among corn sources. Reducing particle size of both corn sources tended to increase litter ADG and weaning weights.
PSIV-21 Impact of Increasing Standardized Ileal Digestible valine:lysine in Diets Containing 30% Dried Distillers Grains with Solubles on Early Grow-finish Pig Performance
Authors: Clizer David A , Samuel Ryan S , Cline Paul M
Published: May 2021

Minimal research has investigated the valine (Val) requirement in grow-finish pig diets, especially when diets contain dried distillers grains plus solubles (DDGS). Therefore, the objective of this study was to determine the standardized ileal digestible (SID) Val:lysine (Lys) requirement in diets containing 30% DDGS. A total of 2,430 pigs (39.4 ± 0.21 kg) were used in a 28-d trial (15 replicates per treatment). Pens were randomly allotted within block to one of six dietary treatments: 30% DDGS diets with a SID Val:Lys ratio of 60, 65, 70, 75, and 80% or a corn-soybean meal diet (CS). Data were analyzed as a randomized complete block, pair-wise comparisons and single degree of freedom orthogonal polynomials (DDGS diets only) were used to evaluate treatment responses, and pen was the experimental unit. Straight broken line (SBL) and quadratic broken line (QBL) regression analysis was used to estimate the SID Val requirement. Increasing SID Val:Lys up to 70% in 30% DDGS increased final BW, ADG and G:F (quadratic; P < 0.001), while increasing SID Val:Lys up to 75% in DDGS diets increased ADFI (quadratic; P < 0.001) Pigs fed CS had greater (P < 0.032) ADG, G:F, and ADFI compared to diets containing 30% DDGS except for cumulative ADFI of pigs receiving diets with 75% SID Val:Lys (P = 0.167). The SBL analysis estimated SID Val:Lys requirement at 66.4, 65.4, and 68.0% for ADG, ADFI, and G:F of pigs from 39.4 to 52.9 kg of body weight (BW) and 66.8, 65.7, and 70.4% for the pigs between the BW of 52.9 and 66.4 kg. The QBL analysis revealed a similar SID Val:Leucine ratio of 52.2% for G:F in both periods. This data suggests a SID Val:Lys requirement of 66.6% in diets containing 30% DDGS for pigs between the BW of 39.4 to 66.4 kg. Increasing the SID Val:Lys ratio in 30% DDGS diets did not improve performance congruent to CS fed pigs.