bluuebunny/crossref_metadata_2025 huggingface dataset:
Mathematical Modeling of the Influence of the Karman Vortex Street on Mass Transfer in Electromembrane Systems
Authors: Uzdenova Aminat , Kovalenko Anna , Prosviryakov Evgeniy , Urtenov Makhamet
Published: March 2023

In electromembrane systems, the transfer of ions near ion-exchange membranes causes concentration polarization, which significantly complicates mass transfer. Spacers are used to reduce the effect of concentration polarization and increase mass transfer. In this article, for the first time, a theoretical study is carried out, using a two-dimensional mathematical model, of the effect of spacers on the mass transfer process in the desalination channel formed by anion-exchange and cation-exchange membranes under conditions when they cause a developed Karman vortex street. The main idea is that, when the separation of vortices occurs on both sides in turn from the spacer located in the core of the flow where the concentration is maximum, the developed non-stationary Karman vortex street ensures the flow of the solution from the core of the flow alternately into the depleted diffusion layers near the ion-exchange membranes. This reduces the concentration polarization and, accordingly, increases the transport of salt ions. The mathematical model is a boundary value problem for the coupled system of Nernst–Planck–Poisson and Navier–Stokes equations for the potentiodynamic regime. The comparison of the current–voltage characteristics calculated for the desalination channel with and without a spacer showed a significant increase in the intensity of mass transfer due to the development of the Karman vortex street behind the spacer.
Eutectic MoNbTa(WC)x Composites with Excellent Elevated Temperature Strength
Authors: Kang Kejia , Wang Xiao , Zhou Weibing , Li Peibo , Huang Zihao , Luo Guoqiang , Shen Qiang , Zhang Lianmeng
Published: March 2023

To develop materials with a promising utilization future in the extreme environments of aerospace, the MoNbTa(WC)x composites were prepared by vacuum arc melting, of which the crystal structure, microstructure, and compression properties at elevated temperature were investigated. The MoNbTa(WC)x composites had eutectic structures that consisted of body-centered cubic (BCC) phase and eutectoid structures. The lamellar fine eutectoid structures were composed of BCC-structured high entropy alloy (HEA) Mo-Nb-Ta-W and FCC-structured carbide Mo-Nb-Ta-W-C. It was demonstrated that the ductility and elevated temperature strength was enhanced simultaneously combined with the effect of eutectic structures and WC addition. The optimal true yield strength and true fracture strain reached 1205 MPa and 29.2% in MoNbTa(WC)0.9 at 1200 °C, meanwhile, the fracture strain at ambient temperature was 13.96%. Distinct strain hardening was observed at the initial deformation stage of MoNbTa(WC)0.9 at 1200 °C. The compression performances of MoNbTa(WC)x were superior in comparison with most refractory high entropy alloys.
Metabolically Active Zones Involving Fatty Acid Elongation Delineated by DESI-MSI Correlate with Pathological and Prognostic Features of Colorectal Cancer
Authors: Kaufmann Martin , Iaboni Natasha , Jamzad Amoon , Hurlbut David , Ren Kevin Yi Mi , Rudan John F. , Mousavi Parvin , Fichtinger Gabor , Varma Sonal , Caycedo-Marulanda Antonio , Nicol Christopher J. B.
Published: March 2023

Colorectal cancer (CRC) is the second leading cause of cancer deaths. Despite recent advances, five-year survival rates remain largely unchanged. Desorption electrospray ionization mass spectrometry imaging (DESI) is an emerging nondestructive metabolomics-based method that retains the spatial orientation of small-molecule profiles on tissue sections, which may be validated by ‘gold standard’ histopathology. In this study, CRC samples were analyzed by DESI from 10 patients undergoing surgery at Kingston Health Sciences Center. The spatial correlation of the mass spectral profiles was compared with histopathological annotations and prognostic biomarkers. Fresh frozen sections of representative colorectal cross sections and simulated endoscopic biopsy samples containing tumour and non-neoplastic mucosa for each patient were generated and analyzed by DESI in a blinded fashion. Sections were then hematoxylin and eosin (H and E) stained, annotated by two independent pathologists, and analyzed. Using PCA/LDA-based models, DESI profiles of the cross sections and biopsies achieved 97% and 75% accuracies in identifying the presence of adenocarcinoma, using leave-one-patient-out cross validation. Among the m/z ratios exhibiting the greatest differential abundance in adenocarcinoma were a series of eight long-chain or very-long-chain fatty acids, consistent with molecular and targeted metabolomics indicators of de novo lipogenesis in CRC tissue. Sample stratification based on the presence of lympovascular invasion (LVI), a poor CRC prognostic indicator, revealed the abundance of oxidized phospholipids, suggestive of pro-apoptotic mechanisms, was increased in LVI-negative compared to LVI-positive patients. This study provides evidence of the potential clinical utility of spatially-resolved DESI profiles to enhance the information available to clinicians for CRC diagnosis and prognosis.
Dependence of Monocrystalline Sapphire Dicing on Crystal Orientation Using Picosecond Laser Bessel Beams
Authors: Wen Qiuling , Chen Jinhong , Huang Guoqin , Cui Changcai , Mu Dekui
Published: March 2023

Dicing is a critical step in the manufacturing process for the application of sapphire. In this work, the dependence of sapphire dicing on crystal orientation using picosecond Bessel laser beam drilling combined with mechanical cleavage was studied. By using the above method, linear cleaving with on debris and zero tapers was realized for the A1, A2, C1, C2, and M1 orientations, except for the M2 orientation. The experimental results indicated that characteristics of Bessel beam-drilled microholes, fracture loads, and fracture sections of sapphire sheets were strongly dependent on crystal orientation. No cracks were generated around the micro holes when laser scanned along the A2 and M2 orientations, and the corresponding average fracture loads were large, 12.18 N and 13.57 N, respectively. While along the A1, C1, C2, and M1 orientations, laser-induced cracks extended along the laser scanning direction, resulting in a significant reduction in fracture load. Furthermore, the fracture surfaces were relatively uniform for A1, C1, and C2 orientations but uneven for A2 and M1 orientations, with a surface roughness of about 1120 nm. In addition, curvilinear dicing without debris or taper was achieved to demonstrate the feasibility of Bessel beams.
Improving the Thickness Uniformity of Micro Gear by Multi-Step, Self-Aligned Lithography and Electroforming
Authors: Wang Huan , Xie Jing , Fan Tao , Sun Dapeng , Li Chaobo
Published: March 2023

The thickness nonuniformity of an electroformed layer is a bottleneck problem for electroformed micro metal devices. In this paper, a new fabrication method is proposed to improve the thickness uniformity of micro gear, which is the key element of various microdevices. The effect of the thickness of the photoresist on the uniformity was studied by simulation analysis, which showed that as the thickness of the photoresist increased, the thickness nonuniformity of the electroformed gear should decrease due to the reduced edge effect of the current density. Differently from the traditional method performed by one-step front lithography and electroforming, multi-step, self-aligned lithography and electroforming are used to fabricate micro gear structures in proposed method, which intermittently keeps the thickness of photoresist from decreasing during processes of alternate lithography and electroforming. The experimental results show that the thickness uniformity of micro gear fabricated by the proposed method was improved by 45.7% compared with that fabricated by the traditional method. Meanwhile, the roughness of the middle region of the gear structure was reduced by 17.4%.
Dynamic Characteristics of a Small-Size Beam Mounted on an Accelerating Structure
Authors: Ali Sajid , Hawwa Muhammad A.
Published: March 2023

This study focuses on the nonlinear vibration of a small-size beam hosted in a high-speed moving structure. The equation of the beam’s motion is derived using the coordinate transformation. The small-size effect is introduced by applying the modified coupled stress theory. The equation of motion involves quadratic and cubic terms due to mid-plane stretching. Discretization of the equation of motion is achieved via the Galerkin method. The impact of several parameters on the non-linear response of the beam is investigated. Bifurcation diagrams are used to investigate the stability of the response, whereas softening/hardening characteristics of the frequency curves are used as an indication of nonlinearity. Results indicate that increasing the magnitude of the applied force tends to signify the nonlinear hardening behavior. In terms of the periodicity of the response, at a lower amplitude of the applied force, the response appears to be a one-period stable oscillation. Increasing the length scale parameter, the response moves from chaotic to period-doubling to the stable one-period response. The impact of the axial acceleration of the moving structure on the stability as well as on the nonlinearity of the response of the beam is also investigated.
Hall Effect at the Focus of an Optical Vortex with Linear Polarization
Authors: Kotlyar Victor V. , Kovalev Alexey A. , Kozlova Elena S. , Telegin Alexey M.
Published: March 2023

The tight focusing of an optical vortex with an integer topological charge (TC) and linear polarization was considered. We showed that the longitudinal components of the spin angular momentum (SAM) (it was equal to zero) and orbital angular momentum (OAM) (it was equal to the product of the beam power and the TC) vectors averaged over the beam cross-section were separately preserved during the beam propagation. This conservation led to the spin and orbital Hall effects. The spin Hall effect was expressed in the fact that the areas with different signs of the SAM longitudinal component were separated from each other. The orbital Hall effect was marked by the separation of the regions with different rotation directions of the transverse energy flow (clockwise and counterclockwise). There were only four such local regions near the optical axis for any TC. We showed that the total energy flux crossing the focus plane was less than the total beam power since part of the power propagated along the focus surface, while the other part crossed the focus plane in the opposite direction. We also showed that the longitudinal component of the angular momentum (AM) vector was not equal to the sum of the SAM and the OAM. Moreover, there was no summand SAM in the expression for the density of the AM. These quantities were independent of each other. The distributions of the AM and the SAM longitudinal components characterized the orbital and spin Hall effects at the focus, respectively.
The Integration of Field Effect Transistors to Microfluidic Devices
Authors: Oliveira Dhaniella Cristhina de Brito , Costa Fernando Henrique Marques , da Silva José Alberto Fracassi
Published: March 2023

Devices that integrate field effect transistors into microfluidic channels are becoming increasingly promising in the medical, environmental, and food realms, among other applications. The uniqueness of this type of sensor lies in its ability to reduce the background signals existing in the measurements, which interfere in obtaining good limits of detection for the target analyte. This and other advantages intensify the development of selective new sensors and biosensors with coupling configuration. This review work focused on the main advances in the fabrication and application of field effect transistors integrated into microfluidic devices as a way of identifying the potentialities that exist in these systems when used in chemical and biochemical analyses. The emergence of research on integrated sensors is not a recent study, although more recently the progress of these devices is more accentuated. Among the studies that used integrated sensors with electrical and microfluidic parts, those that investigated protein binding interactions seem to be the ones that expanded the most due, among other things, to the possibility of obtaining several physicochemical parameters involved in protein–protein interactions. Studies in this area have a great possibility of advancing innovations in sensors with electrical and microfluidic interfaces in new designs and applications.
Polarisation Control in Arrays of Microlenses and Gratings: Performance in Visible–IR Spectral Ranges
Authors: Mu Haoran , Smith Daniel , Katkus Tomas , Gailevičius Darius , Malinauskas Mangirdas , Nishijima Yoshiaki , Stoddart Paul R. , Ruan Dong , Ryu Meguya , Morikawa Junko , Vasiliev Taras , Lozovski Valeri , Moraru Daniel , Ng Soon Hock , Juodkazis Saulius
Published: March 2023

Microlens arrays (MLAs) which are increasingly popular micro-optical elements in compact integrated optical systems were fabricated using a femtosecond direct laser write (fs-DLW) technique in the low-shrinkage SZ2080TM photoresist. High-fidelity definition of 3D surfaces on IR transparent CaF2 substrates allowed to achieve ∼50% transmittance in the chemical fingerprinting spectral region 2–5 μm wavelengths since MLAs were only ∼10 μm high corresponding to the numerical aperture of 0.3 (the lens height is comparable with the IR wavelength). To combine diffractive and refractive capabilities in miniaturised optical setup, a graphene oxide (GO) grating acting as a linear polariser was also fabricated by fs-DLW by ablation of a 1 μm-thick GO thin film. Such an ultra-thin GO polariser can be integrated with the fabricated MLA to add dispersion control at the focal plane. Pairs of MLAs and GO polarisers were characterised throughout the visible–IR spectral window and numerical modelling was used to simulate their performance. A good match between the experimental results of MLA focusing and simulations was achieved.
Multi-Criteria Analysis for Circular Economy Promotion in the Management of Tailings Dams: A Case Study
Authors: Monardes Vinka , Sepúlveda Juan M.
Published: March 2023

Copper ore is a non-renewable resource with lower ore grades, which means that the extraction of more rock material is required to produce the same amount of copper, implying a greater consumption of materials, reagents, water, and energy. Since there is a greater amount of copper sulfide present in nature, concentration using the bubble flotation method will generate a greater number of tailings. This article discusses the environmental issues resulting from tailings dams and how multi-criteria decision analysis can help prioritize those sites in order to promote circular economy measures to compensate for and reduce the impacts of this type of waste generated by the copper mining industry. This work aims to contribute to this purpose by taking information from abandoned and non-active tailings, which are currently present as a result of the lack of regulations in times prior to environmental obligations and because they are metallurgical waste from old operations that had metal recovery rates that were much lower than the current rates. We propose a model based on the multi-criteria Promethee method to prioritize the tailings dams according to the commercial value of the existing materials in the deposits. A case study with an application of the model to 103 dams in the mining region of Coquimbo in northern Chile is shown.
Post-Subduction Granite Magmatism and Gold-Sulfide Mineralization in the Abu Zawal (Fatira) Area, Eastern Desert, Egypt
Authors: El-Wardany Refaey M. , Jiao Jiangang , Zoheir Basem , Kumral Mustafa , Kaya Mustafa , Abdelnasser Amr
Published: March 2023

Gold-sulfide mineralization in the Abu Zawal (Fatira) mine area, North Eastern Desert of Egypt, is related to porphyritic felsite dikes and elongate silicification zones in granitic rocks. These felsite dikes and the host granitic rocks exhibit major and trace element geochemical features typical of calc-alkaline and metaluminous I-type granites, likely originated in a late-orogenic setting. Their geochemical characteristics along with their fractionated LREE relative to HREE patterns imply either formation in a subduction-related environment or generation from subduction-modified source materials. Partial melting of subduction-metasomatized lower crustal rocks during extension following the lithospheric thickening may account for the production of such fertile, high Sr/Y and La/Yb magmas. In the Abu Zawal (Fatira) area, NE-trending altered felsite dikes, hydrothermal breccias, quartz enclaves, and wall-rock replacements are characterized by disseminations of chalcopyrite, pyrite, and rare gold. Alteration mineralogy, dominated by sericite, drusy quartz, kaolinite, calcite, and specular hematite, combined with the available fluid inclusion data suggests moderate to low temperature and near neutral pH conditions. The geochemical data of the altered wallrocks and mass balance calculations indicate significant mass losses in the altered rocks consistent with fluid/wallrock ratios higher than unity and near neutral pH conditions. Considering that the silica-rich host rocks, hydrothermal alteration, and sulfide-bearing hydrothermal quartz breccia in Fatira mine area were intuitively related to sulfur-saturated, oxidized felsic magmatism and associated hydrothermal systems, they are most likely linked to the post-subduction felsite porphyries (post-Hammamat felsites ~ 607 Ma), or pertaining to the late phases of the subaerial high-K calc-alkaline volcanics (Dokhan Volcanics ≤ 620 Ma).
Categorization of Mining Materials for Restoration Projects by Means of Pollution Indices and Bioassays
Authors: Ferri-Moreno Inmaculada , Barquero-Peralbo Jose Ignacio , Andreu-Sánchez Oscar , Higueras Pablo , Roca-Pérez Luis , García-Lorenzo Mari Luz , Esbrí Jose María
Published: March 2023

Sulfide mining wastes may lead to severe environmental and human health risks. This study aims to use geochemical and ecotoxicological indicators for the assessment of the ecological risks of potentially toxic elements (PTEs) in the San Quintín mining group to categorize wastes prior to mining restoration. Ecotoxicity was evaluated using crustacean (Dahpnia magna, Thamnocephalus platyurus) and algae (Raphidocelis subcapitata) bioassays. The geochemical and mineralogical results suggested that the mining residues underwent intense weathering processes, with active processes of acidity generation and metal mobility. Total PTEs concentrations indicated that the mining materials were extremely polluted, with Pb, Zn and Cd geoaccumulation index (Igeo) values higher than 5 in more than 90% of the samples. The pollution load index (PLI) showed average values of 18.1, which classifies them as very highly polluted. The toxicity tests showed a higher toxicity for plants than crustaceans, being the highest values of toxicity related to toxic elements (Pb, Cd and Zn), electrical conductivity and to pH. This paper presents for the first time the combination of indices in the categorization of mining waste prior to its restoration. The combination of them has made it possible to categorize the waste and adapt the restoration and remediation procedures.
Modeling of Sand Triaxial Specimens under Compression: Introducing an Elasto-Plastic Finite Element Model to Capture the Impact of Specimens’ Heterogeneity
Authors: Song Ahran , Pineda-Contreras Alma Rosa , Medina-Cetina Zenon
Published: March 2023

This paper follows up on a series of reference papers that inspired MDPI’s Topic “Stochastic Geomechanics: From Experimentation to Forward Modeling”, where global and local deformation effects on sand specimens are fully described from high-resolution boundary displacement fields, as supported by a comprehensive experimental database (which includes varying degrees of specimen’s heterogeneity) that is available to the scientific community for further study. This paper presents an elasto-plastic comparative analysis of different finite element models reproducing different sand specimen heterogeneity configurations as follows: loose, dense, and half-dense half-loose specimens. The experimental conditions for these specimens’ heterogeneity configurations were simulated with an axisymmetric finite element model. To characterize the stress-strain response obtained from the experiments, an elasto-plastic constitutive model with strain-hardening and softening laws was adopted to reproduce the sand specimens’ mechanistic response. An expert-based calibration of the numerical models accounted for both global and local effects by making use of global observations captured by the triaxial point sensors (i.e., axial force and displacement) and by local observations captured by 3D digital image correlation analysis (i.e., 3D boundary displacement fields). Results show that predictions of the proposed numerical models are in good agreement with the experimental observations, both global and local responses. The combined use of global and local observations to calibrate sand triaxial specimens sets the basis for a more comprehensive parameterization process. For the first model set, three experiments were assumed with homogeneous materials. While both dense and loose models showed good agreement with the experiments, the displacement field prediction of the half-dense half-loose layered model identified limitations in reproducing heterogeneous configurations. Afterward, the second set compared and analyzed the half-dense half-loose layered models by implementing a heterogeneous model, showing significantly better model predictions (i.e., after the implementation of the heterogeneous model, which accounts for a transition zone between the upper and lower segments).
Geochronology and Geochemistry Characteristics of Dongcao Muscovite Granite in the Yifeng Area, Jiangxi Province, China: Implications for Petrogenesis and Mineralization
Authors: Ouyang Yongpeng , Zeng Runling , Meng Delei , Li Tongfei , Wei Jin
Published: March 2023

Dongcao muscovite granite, as the product of the second stage of the magmatic intrusion of the Ganfang composite pluton, is closely related to the mineralization of Li–Nb–Ta rare metals in the Yifeng area. This paper aims to discuss the diagenetic age, evolutionary process, and relationship with the rare metal mineralization of Dongcao muscovite granite by using petrographic, cassiterite U–Pb dating and geochemical analyses. Petrographic analysis shows that the lithology of the Dongcao muscovite granite is medium– to fine–grained muscovite monzogranite. The cassiterite U–Pb dating results show that the diagenetic age of the Dongcao muscovite granite is 139.7 ± 6.7 Ma, which is Early Cretaceous. The geochemical analysis indicates that the rock is characterized by high Si, abundant aluminum alkalis, low Ca and Fe, and low Mg, which indicates that this granite is a strongly peraluminous rock. Moreover, the Dongcao muscovite granite is enriched with Rb, U, Ta, Pb, P, and Hf and depleted of Ba, Sr, Ti, and rare earth elements (REEs), with a tetrad effect of REEs. Based on this analysis, the Dongcao muscovite granite is a highly differentiated granite that formed in the tectonic transition from continental collisional to post–collisional settings related to the subduction of the Paleo–Pacific plate. A high degree of crystallization differentiation occurred at the early stage of magmatic evolution, resulting in the initial enrichment of Li–Nb–Ta–Sn. The melt–fluid interaction in the late stage is significant to the high enrichment of Li–Nb–Ta–Sn until the final mineralization.
Established and Emerging Methods for Protecting Linear DNA in Cell-Free Expression Systems
Authors: Fochtman Trevor J. , Oza Javin P.
Published: March 2023

Cell-free protein synthesis (CFPS) is a method utilized for producing proteins without the limits of cell viability. The plug-and-play utility of CFPS is a key advantage over traditional plasmid-based expression systems and is foundational to the potential of this biotechnology. A key limitation of CFPS is the varying stability of DNA types, limiting the effectiveness of cell-free protein synthesis reactions. Researchers generally rely on plasmid DNA for its ability to support robust protein expression in vitro. However, the overhead required to clone, propagate, and purify plasmids reduces the potential of CFPS for rapid prototyping. While linear templates overcome the limits of plasmid DNA preparation, linear expression templates (LETs) were under-utilized due to their rapid degradation in extract based CFPS systems, limiting protein synthesis. To reach the potential of CFPS using LETs, researchers have made notable progress toward protection and stabilization of linear templates throughout the reaction. The current advancements range from modular solutions, such as supplementing nuclease inhibitors and genome engineering to produce strains lacking nuclease activity. Effective application of LET protection techniques improves expression yields of target proteins to match that of plasmid-based expression. The outcome of LET utilization in CFPS is rapid design–build–test–learn cycles to support synthetic biology applications. This review describes the various protection mechanisms for linear expression templates, methodological insights for implementation, and proposals for continued efforts that may further advance the field.
Effect of a-SiCxNy:H Encapsulation on the Stability and Photoluminescence Property of CsPbBr3 Quantum Dots
Authors: Lin Zewen , Lin Zhenxu , Guo Yanqing , Wu Haixia , Song Jie , Zhang Yi , Zhang Wenxing , Li Hongliang , Hou Dejian , Huang Rui
Published: March 2023

The effect of a-SiCxNy:H encapsulation layers, which are prepared using the very-high-frequency plasma-enhanced chemical vapor deposition (VHF-PECVD) technique with SiH4, CH4, and NH3 as the precursors, on the stability and photoluminescence of CsPbBr3 quantum dots (QDs) were investigated in this study. The results show that a-SiCxNy:H encapsulation layers containing a high N content of approximately 50% cause severe PL degradation of CsPbBr3 QDs. However, by reducing the N content in the a-SiCxNy:H layer, the PL degradation of CsPbBr3 QDs can be significantly minimized. As the N content decreases from around 50% to 26%, the dominant phase in the a-SiCxNy:H layer changes from SiNx to SiCxNy. This transition preserves the inherent PL characteristics of CsPbBr3 QDs, while also providing them with long-term stability when exposed to air, high temperatures (205 °C), and UV illumination for over 600 days. This method provided an effective and practical approach to enhance the stability and PL characteristics of CsPbBr3 QD thin films, thus holding potential for future developments in optoelectronic devices.
A Novel Drastic Peptide Genetically Adapted to Biomimetic Scaffolds “Delivers” Osteogenic Signals to Human Mesenchymal Stem Cells
Authors: Mantsou Aglaia , Papachristou Eleni , Keramidas Panagiotis , Lamprou Paraskevas , Pavlidis Alexandros , Papi Rigini , Dimitriou Katerina , Aggeli Amalia , Choli-Papadopoulou Theodora
Published: March 2023

This work describes the design, preparation, and deep investigation of “intelligent nanobiomaterials” that fulfill the safety rules and aim to serve as “signal deliverers” for osteogenesis, harboring a specific peptide that promotes and enhances osteogenesis at the end of their hydrogel fibers. The de novo synthesized protein fibers, besides their mechanical properties owed to their protein constituents from elastin, silk fibroin and mussel-foot adhesive protein-1 as well as to cell-attachment peptides from extracellular matrix glycoproteins, incorporate the Bone Morphogenetic Protein-2 (BMP2) peptide (AISMLYLDEN) that, according to our studies, serves as “signal deliverer” for osteogenesis. The osteogenetic capacity of the biomaterial has been evidenced by investigating the osteogenic marker genes ALP, RUNX2, Osteocalcin, COL1A1, BMPR1A, and BMPR2, which were increased drastically in cells cultured on scaffold-BMP2 for 21 days, even in the absence of osteogenesis medium. In addition, the induction of phosphorylation of intracellular Smad-1/5 and Erk-1/2 proteins clearly supported the osteogenetic capacity of the biomaterial.
Effectiveness of a Smartphone App (e-12HR) in Improving Adherence to the Mediterranean Diet in Spanish University Students by Age, Gender, Field of Study, and Body Mass Index: A Randomized Controlled Trial
Authors: Béjar Luis M. , Mesa-Rodríguez Pedro , Quintero-Flórez Angélica , Ramírez-Alvarado María del Mar , García-Perea María Dolores
Published: March 2023

There is an urgent need to implement intervention programs to promote adherence to the Mediterranean diet (AMD) in university students to prevent non-communicable diseases. A powerful tool for this is smartphone apps. Furthermore, it is necessary to determine the subgroups that are most likely to benefit from these technologies. The objective is to evaluate the effectiveness of an app (e-12HR) at improving AMD in a sample of Spanish university students and different strata. The study method was a controlled and randomized clinical trial over a four-week follow-up period and involving 385 participants (76.9% women). The participants were in two parallel groups: the control group (CG) and the intervention group (IG), with only the IG receiving feedback to improve their AMD. There were significant statistical improvements (with higher values in the IG) at week four, after no significant statistical differences at baseline (Week One): in the whole sample: +25.7% AMD index and +74.5% percentage with moderate/high AMD index. In the subgroups, seven of eight subgroups, ranging in AMD index from +17.8% (≥20 years) to +33.0% (<20 years); and for males, in weeks two (+27.9%) and three (+23.9%), but not at week four. In conclusion, e-12HR could improve AMD among university students (in the total sample and all subgroups, except ≥25 kg/m2).
Long-Term Impact of Multiple Micronutrient Supplementation on Micronutrient Status, Hemoglobin Level, and Growth in Children 24 to 59 Months of Age: A Non-Randomized Community-Based Trial from Pakistan
Authors: Khan Aslam , Ul-Haq Zia , Fatima Sadia , Ahmed Jawad , Alobaid Hussah M. , Fazid Sheraz , Muhammad Nawshad , Garzon Cecilia , Ihtesham Yasir , Habib Ijaz , Tanimoune Mahamadou , Iqbal Khalid , Arshad Muhammad , Safi Sher Zaman
Published: March 2023

Cost-effective interventions are needed to address undernutrition, particularly micronutrient deficiencies, which are common in children under the age of five in low- and middle-income countries. A community-based, non-randomized clinical trial was undertaken in the Kurram district of Khyber Pakhtunkhwa from January 2018 to June 2019, to evaluate the effect of locally produced micronutrient powder (local name: Vita-Mixe) on plasma micronutrient status, hemoglobin level, and anthropometric outcomes. Children aged 24–48 months old were recruited and allocated to the intervention and control arm of the study. The enrolled children in the intervention arm received one micronutrient powder (MNP) sachet for consumption on alternate days for 12 months. To assess the impact of the intervention on plasma levels of zinc, vitamin D, vitamin A, and hemoglobin level, blood samples were taken at baseline and after one year following the intervention. The analysis was conducted using Enzyme-Linked Immunosorbent Assay (ELISA), atomic absorption spectrometry, and an automated hematology analyzer. For the impact on growth parameters, the anthropometric assessment was performed using WHO standard guidelines. A 24 h dietary recall interview was used to assess the nutrient intake adequacy. Results showed that in the intervention arm, children had on average a 7.52 ng/mL (95% CI 5.11–9.92, p-value < 0.001) increase in the plasma level of vitamin A, 4.80 ng/mL (95% CI 1.63–7.95, p-value < 0.002) increase in vitamin D levels and 33.85 µg/dL (95% CI 24.40–43.30, p-value < 0.001) increase in the plasma zinc level, as well as a 2.0g/dL (95% CI 1.64–2.40, p-value < 0.001) increase in hemoglobin level. Statistically significant improvement was observed in the weight-for-height z-score (WHZ) (from −1.0 ± 0.88 to −0.40 ± 1.01, p < 0.001) and weight-for-age z-score (WAZ) (from −1.40 ± 0.50 to −1.05 ± 0.49, p < 0.001) in the intervention group compared to the control group. No statistically significant change was observed in the height-for-age z-score (HAZ) in the intervention group (p = 0.93). In conclusion, micronutrient powder supplementation is a cost-effective intervention to improve the micronutrient status, hemoglobin level, and growth parameters in under-five children, which can be scaled up in the existing health system to address the alarming rates of undernutrition in Pakistan and other developing countries.
Integration of Metabolomics and Transcriptomics to Reveal the Metabolic Characteristics of Exercise-Improved Bone Mass
Authors: Hou Jin-Li , Yang Wan-Yu , Zhang Qiong , Feng Hao , Wang Xiao-Bao , Li Hui , Zhou Sheng , Xiao Su-Mei
Published: March 2023

(1) Background: Exercise is effective in promoting and maintaining bone mass. The aim of this study was to detect the exercise-induced metabolic changes in bone tissue of zebrafish. (2) Methods: Thirty-eight zebrafish (Danio rerio, six months old) were analyzed. The exercise group (n = 19) received 8 weeks of counter-current swimming training. The control group (n = 19) was not subjected to exercise. Mineralization was quantified, and alkaline phosphatase (Alp) and anti-tartrate acid phosphatase (Trap) activities were estimated (n = 12). The metabolomics (n = 12) and transcriptomics (n = 14) data of bone tissue were used for the integration analyses. (3) Results: The results showed that the exercise training improved the bone mineralization of zebrafish, e.g., the exercise group (5.74 × 104 ± 7.63 × 103) had a higher mean optical density than the control group (5.26 × 104 ± 8.56 × 103, p = 0.046) for the caudal vertebrae. The amount of mineralized matrix in scales of the exercised zebrafish was also higher (0.156 ± 0.012 vs. 0.102 ± 0.003, p = 0.005). Both histological staining and biochemical analysis revealed increased Alp activity (0.81 ± 0.26 vs. 0.76 ± 0.01, p = 0.002) and decreased Trap activity (1.34 ± 0.01 vs. 1.36 ± 0.01, p = 0.005) in the exercise group. A total of 103 different metabolites (DMs, VIP ≥ 1, fold change (FC) ≥ 1.20 or ≤0.83, p < 0.050) were identified. Alanine, aspartate and glutamate metabolism, β-alanine metabolism, pyrimidine metabolism, and pantothenate and CoA biosynthesis were the significantly enriched metabolic pathways (p < 0.050). A total of 35 genes (q ≤ 0.050 (BH), |Log2FC| ≥ 0.5) were coenriched with the 103 DMs in the four identified pathways. Protein–protein interaction network analysis of the 35 genes showed that entpd3, entpd1, and cmpk2 were the core genes. (4) Conclusions: The results of this study suggest that alanine, aspartate and glutamate metabolism, β-alanine metabolism, pyrimidine metabolism, and pantothenate and CoA biosynthesis contributed to exercise-induced improvements in bone mass.
Factors Influencing Undernutrition among Female Adolescent Students in Darchula District, Nepal
Authors: Giri Deepika , Vajanapoom Nitaya , Langkulsen Uma
Published: March 2023

Failure to understand and address the problem of malnutrition and its associated factors among female adolescents could lead to a vicious cycle of intergenerational malnutrition. A cross-sectional study was conducted in six secondary schools of four rural municipalities in Darchula District, Nepal. Data collection for the study was conducted from November 2021 to February 2022. Four hundred female adolescent students aged 15 to 19 years old were selected using quota sampling. The study aims to examine the prevalence of malnutrition by assessing different levels of body mass index (BMI) that is thinness (BMI less than 18.5 kg/m2), normal (18.5 kg/m2 to 24.9 kg/m2), overweight (25 kg/m2 to 29.9 kg/m2) and obese (BMI more than or equal to 30 kg/m2). Along with BMI, factors associated with undernutrition, here identified as thinness, are assessed using a structured questionnaire. Additionally, key informant interviews and review of interventions was performed to understand the gaps in nutrition-related policies and programs of school going adolescents in the study district. Quantitative data analysis included a prevalence study and chi-square test along with simple and multiple logistic regression to obtain crude and adjusted odds ratio at 95% confidence interval for the significant factors with p < 0.05 identified in the chi-square test. Thematic analysis and reviews were used for the synthesis of qualitative data. The results show a 24.7% prevalence of thinness in the study participants. Pre-mensuration status (OR = 5.015, CI = 1.257–20.011, p < 0.022), father having a monthly paying job (OR = 4.384, CI = 1.135–16.928, p < 0.032), father’s foreign employment (OR = 6.96, CI = 1.649–29.377, p < 0.008), household (HH) food insecurity status (OR = 2.079, CI = 1.182–3.658, p < 0.011) and grain/roots/tuber as most commonly bought food (OR = 9.487, CI = 1.182–76.138, p < 0.034) were found to be significantly associated with thinness. Information from the qualitative part identified gaps in existing interventions for an improved nutritional outcome among school going adolescent females. Further studies to understand the nutritional practices and its contributory factors in relation to thinness is encouraged. Stakeholders are urged to effectively address the shortcomings in existing interventions and adopt a more adolescent-centered approach to enhance the nutritional status of female adolescents.
HMOs Exert Marked Bifidogenic Effects on Children’s Gut Microbiota Ex Vivo, Due to Age-Related Bifidobacterium Species Composition
Authors: Bajic Danica , Wiens Frank , Wintergerst Eva , Deyaert Stef , Baudot Aurélien , Van den Abbeele Pieter
Published: March 2023

Prebiotics are substrates that are selectively utilized by host microorganisms, thus conferring a health benefit. There is a growing awareness that interpersonal and age-dependent differences in gut microbiota composition impact prebiotic effects. Due to the interest in using human milk oligosaccharides (HMOs) beyond infancy, this study evaluated how HMOs [2’Fucosyllactose (2’FL), Lacto-N-neotetraose (LNnT), 3’Sialyllactose (3’SL), 6’Sialyllactose (6’SL)] and blends thereof affect the microbiota of 6-year-old children (n = 6) and adults (n = 6), compared to prebiotics inulin (IN) and fructooligosaccharides (FOS). The ex vivo SIFR® technology was used, given its demonstrated predictivity in clinical findings. First, HMOs and HMO blends seemed to maintain a higher α-diversity compared to FOS/IN. Further, while 2′FL/LNnT were bifidogenic for both age groups, 3′SL/6′SL and FOS/IN were exclusively bifidogenic for children and adults, respectively. This originated from age-related differences in microbiota composition because while 3′SL/6′SL stimulated B. pseudocatenulatum (abundant in children), FOS/IN enhanced B. adolescentis (abundant in adults). Moreover, all treatments significantly increased acetate, propionate and butyrate (only in adults) with product- and age-dependent differences. Among the HMOs, 6′SL specifically stimulated propionate (linked to Bacteroides fragilis in children and Phocaeicola massiliensis in adults), while LNnT stimulated butyrate (linked to Anaerobutyricum hallii in adults). Indole-3-lactic acid and 3-phenyllactic acid (linked to immune health) and gamma-aminobutyric acid (linked to gut-brain axis) were most profoundly stimulated by 2′FL and HMO blends in both children and adults, correlating with specific Bifidobacteriaceae. Finally, 2′FL/LNnT increased melatonin in children, while 3′SL remarkably increased folic acid in adults. Overall, age-dependent differences in microbiota composition greatly impacted prebiotic outcomes, advocating for the development of age-specific nutritional supplements. HMOs were shown to be promising modulators in the adult, and particularly the children’s microbiota. The observed HMO-specific effects, likely originating from their structural heterogeneity, suggest that blends of different HMOs could maximize treatment effects.
A Systematic Study on Zinc-Related Metabolism in Breast Cancer
Authors: Qu Zheng , Liu Qiang , Kong Xiangyi , Wang Xiangyu , Wang Zhongzhao , Wang Jing , Fang Yi
Published: March 2023

Breast cancer has become the most common cancer worldwide. Despite the major advances made in the past few decades in the treatment of breast cancer using a combination of chemotherapy, endocrine therapy, and immunotherapy, the genesis, treatment, recurrence, and metastasis of this disease continue to pose significant difficulties. New treatment approaches are therefore urgently required. Zinc is an important trace element that is involved in regulating various enzymatic, metabolic, and cellular processes in the human body. Several studies have shown that abnormal zinc homeostasis can lead to the onset and progression of various diseases, including breast cancer. This review highlights the role played by zinc transporters in pathogenesis, apoptosis, signal transduction, and potential clinical applications in breast cancer. Additionally, the translation of the clinical applications of zinc and associated molecules in breast cancer, as well as the recent developments in the zinc-related drug targets for breast cancer treatment, is discussed. These developments offer novel insights into understanding the concepts and approaches that could be used for the diagnosis and management of breast cancer.
The Role of Diet in Children with Psoriasis: Emerging Evidence and Current Issues
Authors: De Simoni Edoardo , Rizzetto Giulio , Molinelli Elisa , Capodaglio Irene , Offidani Annamaria , Simonetti Oriana
Published: March 2023

Psoriasis is an immune-mediated inflammatory systemic disease with skin tropism and chronic relapsing course; it is associated with an increased cardiovascular risk and with many metabolic comorbidities, emerging during childhood in 22–33% of cases. Diet influences the presentation and the clinical course of inflammatory diseases, including psoriasis; in particular, it was shown that a Mediterranean, gluten-free, or low-calorie diet may positively affect disease control in adult patients with psoriasis and adequate pharmacological therapy. These three dietary regimens may play a role also in children with psoriasis. It has been demonstrated that pediatric psoriasis is associated with psychological stress, celiac disease, and obesity, which may be positively influenced by these dietary regimens, respectively. Therefore, the expertise of multiple health figures (gastroenterologists, nutritionists, pediatricians, dermatologists) is required to plan a tailor-made dietary strategy, ensuring good growth, through an adequate intake of essential micro- and macronutrients and, at the same time, impacting the pro-inflammatory biochemical profile and on the associated cardiovascular risk of psoriasis disease.
Fish Oil Supplementation Improves the Repeated-Bout Effect and Redox Balance in 20–30-Year-Old Men Submitted to Strength Training
Authors: Barquilha Gustavo , Dos Santos Cesar Miguel Momesso , Caçula Kim Guimaraes , Santos Vinícius Coneglian , Polotow Tatiana Geraldo , Vasconcellos Cristina Vardaris , Gomes-Santos José Alberto Fernandes , Rodrigues Luiz Eduardo , Lambertucci Rafael Herling , Serdan Tamires Duarte Afonso , Levada-Pires Adriana Cristina , Hatanaka Elaine , Cury-Boaventura Maria Fernanda , de Freitas Paulo Barbosa , Pithon-Curi Tania Cristina , Masi Laureane Nunes , Barros Marcelo Paes , Curi Rui , Gorjão Renata , Hirabara Sandro Massao
Published: March 2023

Herein, we investigated the effect of fish oil supplementation combined with a strength-training protocol, for 6 weeks, on muscle damage induced by a single bout of strength exercise in untrained young men. Sixteen men were divided into two groups, supplemented or not with fish oil, and they were evaluated at the pre-training period and post-training period. We investigated changes before and 0, 24, and 48 h after a single hypertrophic exercise session. Creatine kinase (CK) and lactate dehydrogenase (LDH) activities, plasma interleukin-6 (IL-6) and C-reactive protein (CRP) levels, and the redox imbalance were increased in response to the single-bout session of hypertrophic exercises at baseline (pre-training period) and decreased during the post-training period in the control group due to the repeated-bout effect (RBE). The fish oil supplementation exacerbated this reduction and improved the redox state. In summary, our findings demonstrate that, in untrained young men submitted to a strength-training protocol, fish oil supplementation is ideal for alleviating the muscle injury, inflammation, and redox imbalance induced by a single session of intense strength exercises, highlighting this supplementation as a beneficial strategy for young men that intend to engage in strength-training programs.
Circulating Short-Chain Fatty Acids and Non-Alcoholic Fatty Liver Disease Severity in Patients with Type 2 Diabetes Mellitus
Authors: Tsai Hui-Ju , Hung Wei-Chun , Hung Wei-Wen , Lee Yen-Jung , Chen Yo-Chia , Lee Chun-Ying , Tsai Yi-Chun , Dai Chia-Yen
Published: March 2023

(1) Background: Non-alcoholic fatty liver disease (NAFLD) is a major global health concern. The increasing prevalence of NAFLD has been related to type 2 diabetes mellitus (T2D). However, the relationship between short-chain fatty acids (SCFAs) and NAFLD severity is ambiguous in T2D subjects. This study aimed to explore the association of SCFAs with the severity of NAFLD in T2D patients. (2) Methods: We employed echography to examine the severity of hepatic steatosis. The serum levels of nine SCFAs, namely, formate, acetate, propionate, butyrate, isobutyrate, methylbutyrate, valerate, isovalerate, and methylvalerate, were measured using gas chromatography mass spectrometry. (3) Results: A total of 259 T2D patients was enrolled in this cross-sectional study. Of these participants, 117 with moderate to severe NAFLD had lower levels of formate, isobutyrate, and methylbutyrate than the 142 without NAFLD or with mild NAFLD. Lower circulating levels of isobutyrate and methylbutyrate were associated with an increased severity of NAFLD. A relationship between NAFLD severity and circulating isobutyrate and methylbutyrate levels was found independently of a glycated hemoglobin (HbA1C) level of 7.0%. (4) Conclusion: Circulating levels of isobutyrate and methylbutyrate were significantly and negatively correlated with NAFLD severity in the enrolled T2D patients. SCFAs may be related to NAFLD severity in T2D patients.