bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-concept and sensation seeking as predictors of sexual behaviors under the influence of drugs among college students / Autoconcepto y búsqueda de sensaciones como predictores de conductas sexuales bajo los efectos de las drogas en universitarios
Authors: Espada José P. , Antón Fina A. , Torregrosa María Soledad
Published: November 2017

Los jóvenes constituyen una población de alto riesgo frente al VIH /SIDA al ser las conductas sexuales sin protección una de las principales vías de transmisión en la actualidad. El objetivo de este estudio fue analizar en qué medida variables cognitivas predisposicionales como el autoconcepto y la búsqueda de sensaciones, están relacionadas con las relaciones sexuales bajo los efectos de las drogas en jóvenes universitarios.La muestra estuvo compuesta por 339 estudiantes con edades entre18 y 29 años (M = 19.53; DE = 3.27). Los estudiantes cumplimentaron un cuestionario auto-administrado que evaluaba su comportamiento sexual, el consumo de sustancias, el nivel de búsqueda de sensaciones y de autoconcepto. Para analizar la capacidad predictiva de estas variables se elaboraron tres modelos de regresión independientes entre sí en función del tipo de sustancia consumida. El autoconcepto ha resultado ser predictor de las conductas de riesgo bajos los efectos del cannabis y otras drogas, pero no en el caso del alcohol. La búsqueda de sensaciones no resulta ser predictora de mantener relaciones sexuales bajo la influencia de las drogas. AbstractYoungsters represent a high HIV-related risk population becauseunprotected sexual behavior is the mainly cause today. The aim of this study was to assess two cognitive variables, self-concept and sensation seeking, as predictors of sexual risk behavior among college students.The sample included 339 undergraduates aged 18-29 years (M = 19.53; SD = 3.27). The students completed a self-administered questionnaire during the class about their sexual behaviors, drug-taking, and sensation seeking and self-concept scales. Three independent predicting models based on the kind of substance were tested. Self-concept is a predictor of risk sexual behavior under the influence of cannabis and other drugs, but not under the influence of alcohol. Sensation seeking isn’t a predictor of risk sexual behavior under influence of drugs.
Health orientation and drug use in young people. Is really effective current prevention? / Orientación hacia la salud y consumo de drogas en la población juvenil ¿Es realmente eficaz la prevención actual?
Authors: Rial Antonio , Torrado Nair , Varela Jesús
Published: November 2017

A pesar de los esfuerzos realizados en materia de prevención, las cifras de consumo de drogas en nuestro país siguen siendo preocupantes, revelando edades de consumo cada vez más precoces. A día de hoy las explicaciones son muy diversas, aunque no suelen traducirse en soluciones realmente efectivas. El presente trabajo, planteado desde la perspectiva del Marketing Sanitario, intenta encontrar algunas de las claves de esta situación. El trabajo empírico ha consistido en la realización de 683 entrevistas a jóvenes de ambos sexos, de entre 18 y 30 años, residentes en la comunidad gallega. Además de actualizar los datos disponibles referidos a hábitos y prácticas de riesgo, se ha analizado la relación entreambos tipos de variables, en un intento de alcanzar nuevas explicaciones y claves para una mejor prevención del consumo de drogas en esta población. Los resultados obtenidos ponen en entredicho la eficacia de las actuales políticas de prevención. AbstractIn last two decades a big effort has been made in order to prevent drug use, and the number of studies related to consumption of these substances among young people has increased. Nevertheless, the figures are still high, revealing both an increasingly shorter age of initiation. Nowadays, the number of possible explanations for this is large, but they are not targeted towards real solutions. The present study, departing from a Health Marketing perspective, tries to find some key aspects of this situation.The empirical work, consisting of 683 interviews with young men and women, among 18 and 30 years, residents in Galicia, apart from updating the available data about habits and risk behaviors, linking both types of variables in an attempt to reach new plausible explanations and keys, in order to a better prevention of drug use in young people. The results call into question the effectiveness of current prevention policies.
Full number: Volume 8, Number 2 / Número completo: Volumen 8, Número 2
Authors: De diciembre de 2018. Número completo
Published: November 2017

Full number: Volume 8, Number 2 / Número completo: Volumen 8, Número 2.
Radon in the Dwellings: Causes and Prevention
Authors: Khan M Shakir
Published: November 2017

The problem of radon in dwellings gained importance in 1984 after an incidence in Pennsylvania, USA; thereafter several measuring techniques have been developed and national survey programs were started throughout the world for monitoring radon levels by government authorities. The lots of work have done by researchers in this field throughout the world. Several developed countries have given guidelines for initiating action to reduce radon levels if found beyond permissible level. The main aim of this article to provide public awareness about the health hazards posed by radon, which is naturally occurring invisible inert gas in the dwellings that is not be detected by human senses.
Optimal Power Flow Using an Improved Hybrid Differential Evolution Algorithm
Authors: Chen Gonggui , Lu Zhengmei , Zhang Zhizhong , Sun Zhi
Published: November 2017

Objective:

In this paper, an improved hybrid differential evolution (IHDE) algorithm based on differential evolution (DE) algorithm and particle swarm optimization (PSO) has been proposed to solve the optimal power flow (OPF) problem of power system which is a multi-constrained, large-scale and nonlinear optimization problem.

Method:

In IHDE algorithm, the DE is employed as the main optimizer; and the three factors of PSO, which are inertia, cognition, and society, are used to improve the mutation of DE. Then the learning mechanism and the adaptive control of the parameters are added to the crossover, and the greedy selection considering the value of penalty function is proposed. Furthermore, the replacement mechanism is added to the IHDE for reducing the probability of falling into the local optimum. The performance of this method is tested on the IEEE30-bus and IEEE57-bus systems, and the generator quadratic cost and the transmission real power losses are considered as objective functions.

Results:

The simulation results demonstrate that IHDE algorithm can solve the OPF problem successfully and obtain the better solution compared with other methods reported in the recent literatures.
Facial and Orbital Fractures: A Fifteen Years Retrospective Evaluation of North East Sicily Treated Patients
Authors: Runci Michele , De Ponte Francesco Saverio , Falzea Roberto , Bramanti Ennio , Lauritano Floriana , Cervino Gabriele , Famà Fausto , Calvo Alessandro , Crimi Salvatore , Rapisarda Silvia , Cicciù Marco
Published: November 2017

Background:

Orbital fractures are classified as diseases usually related to common midface trauma. It represents the most challenging treatment due to the complex anatomy, physiology, and aesthetic role. A midface trauma involves also the zygomatic complex and the nose, however the orbit fracture seems to be a more frequent disease due to its anatomical features.

Objective:

The purpose of this work is to retrospectively evaluate and record the frequency of the midfacial traumas and orbital fractures observed in the North Eastern Sicily. The results of the present data may be useful for the clinicians in order to recognize the kind of fracture just from the first general visit having a quick diagnosis and management.

Methods:

In the years between 2001 and 2016, about 1200 patients with midfacial trauma and about 100 patients involving the orbital floor have been evaluated. All those patients underwent the surgical fracture reduction and a CT scan follow up control at one month, three months, six months and one year.

Results:

Data showed high percentage of orbital floor, nose and mandibular body and ramus fractures; moreover the most frequent causes of fractures seem to be related to motor vehicle accident, followed by assaults, work and fall.

Conclusion:

The results have highlighted the changing trends in the causes of facial injuries, particularly the increasing incidence of assaults and the falling incidence of motor vehicle accidents in developed countries. The quick diagnosis and management proved fundamental for the successful treatment. Clinicians should be able to recognize the first symptoms in order to avoid possible complications.
Assessment of the Influence of Lagos Bus Rapid Transit Scheme (BRT-Lite) on Road Traffic Crashes (RTC) on Lagos Mainland-Island Corridor
Authors: Ajayi Adeyinka Peter
Published: November 2017

Background:

This paper filled an important gap in the on-going global assessment of Bus Rapid Transit (BRT) operations on Road Traffic Crashes (RTC) safety outcomes on corridors plied.

Introduction:

The work carried out a scientific investigation of the impact of Lagos BRT-Lite(Africa’s first and only BRT scheme) on the achievement of a possible reduction in the incidences of RTC on the 22-kilometer radial highway that connects Mile12 and Lagos Island the traditional central business district.

Methodology:

Secondary data on the incidences of RTC on the corridor between 2002 and 2013 were obtained from Nigeria Police. One-way Analysis of variance between subjects revealed that the advent of BRT operations on the corridor do not have any significant effect in the reported cases of the three categories of RTC examined. Minor (p=. 783), Serious (p= .887) and Fatal (p= .826).

Data Analysis:

Descriptive statistics, however, showed that there has been a general reduction in the incidences of all categories of RTC considered in the period after the commencement of BRT on the corridor.

Conclusion:

The paper concludes by positing that while it might be far-fetched to imply a direct causal relationship between the introduction of BRT and reduction in the cases of RTC on the corridor, it may not be far to suggest that it has definitely impacted safety outcomes as it relates to RTC.
Formulation and In-Vitro Evaluation of Solid Lipid Nanoparticles Containing Levosulpiride
Authors: Singh Sukhwinder , Kamal Sukhmeet Singh , Sharma Amit , Kaur Daljit , Katual Manoj Kumar , Kumar Rajesh
Published: November 2017

Objectives:

The present study aims on preparing Levosulpiride loaded solid lipid nanoparticles (SLNs) to reduce the dose, frequency of dosing, reduce side effects and to increase the bioavailable fraction of drug (<30% orally in general).

Methods:

Levosulpiride was characterized by preformulation studies like physical appearance, melting point, assay, calibration curve, FTIR analysis and DSC analysis. The calibration curve of the drug was prepared in pH 6.8 phosphate buffer. Two lipids (Stearic acid and Palmitic acid) were used as lipid phase to prepare SLNs. Factorial design (23) was applied to formulate 16 formulations (8 for each lipid i.e. SF1-SF8 and PF1-PF8). Levosulpiride SLNs were prepared by solvent evaporation method followed by homogenization.

Results:

The optimized formulations were characterized by particle size analysis, zeta potential analysis, in vitro drug release and drug release kinetics. Drug-excipient interaction in optimized formulation was characterized by FTIR, DSC and TEM analysis.

Conclusion:

On the basis of evaluation parameters, the formulation SF1 (containing Stearic acid) and PF1 (containing Palimitic acid) found to be better formulations amongst their groups with a controlled drug release after a period of 24 hrs.
Measuring Service Quality Expectation and Satisfaction Using Servqual at Hutan Lipur Sungai Congkak
Authors: HASHIM NUR IDZHAINEE , JAINI NORAJLIN , JAMALUDDIN ELY ROUZEE , CHEK SULAIMAN FIRDAUS
Published: November 2017

Recreation activity based on recreational forest has risen as one of the important industries in the Malaysia economy. This area has a great potential for future development predominantly in this modern trend where people are more concern about living a healthy lifestyle and being close to nature. The purpose of this paper is to obtain a better understanding of the extent to which service quality is delivered within Hutan Lipur Sungai Congkak. This study searched the influence of service quality attributes on expectation and satisfaction of recreational forest based on SERVQUAL model. The finding shows that service quality directly impacted visitors’ satisfaction on the five aspects of service quality. Hence, the results of this study provide important theoretical implications for nature-based tourism managers.
Behavioral Program and Exercise Training for Older Persons: Effect on Cardiorespiratory Fitness
Authors: Azizan Azliyana , Justine Maria , Alias Noor Amiera
Published: November 2017

Active participation in exercise may help to improve physical and psychological functions for older people. Regretfully, most of them do not engage in long term exercise due to several barriers. This puts them at high risk of may chronic illness that will limit their activities of daily living. The aim of this study is to determine the effectiveness of the behavioral and exercise program on cardio respiratory fitness among 63 community-dwelling olders. At the end of six months, there is a significant difference for cardio respiratory fitness and exercise engagement. Thus, improved their overall quality of life.
The Effect of Different Locations of Simulated Central Vision Scotoma on Quality of Reading
Authors: Buari Noor Halilah , Nur-Zahirah Muhammad Zahir
Published: November 2017

As a person read, the eyes move to extract information from the printed text. Reading became disturbed when obstructed by the scotoma. The effect of reading and eye movement were investigated between different locations of simulated central vision scotoma. The time to read the text and the eye movements were recorded and tracked among eleven participants. The reading speed showed a significant difference at different locations of central scotoma simulation. The presence of central scotoma affects the quality of reading as the eye moved slowly during reading in order to comprehend the text.
The Determinant of Consumers’ Socio-Economic Attributes on Their Choices of Environmental Friendly Furniture style.
Authors: Kwangsawat Karuna , Rugwongwan Yanin
Published: November 2017

This article presents the determinant of consumers' socio-economic including personality, attitudes, lifestyle and the physical characteristics of the residence. That determines the consumer's demand for environmentally friendly furniture. This is a test of the demand for different types of furniture. According to economic conditions consumer society. The research found that overall style of furniture requirements. The consumers demand modern style furniture, western style, use natural materials to produce, high technology in production and the furniture is cool shade is moderate. The design should take into account the socio-economic conditions of each consumer group. To serve as guidelines for the environmentally friendly design furniture that is suitable to the demand of consumers.
Perceived Depression, Anxiety and Stress Among UiTM Dental Undergraduates in Clinical Years
Authors: Mohd Nayan Noor Aziella , Che Daud Ahmad Zamir , Tengku Jamaluddin Tengku Intan Baizura , Talib Siti Salwa
Published: November 2017

This study investigated the occurrence of depression, anxiety and stress; and identified the possible stress factors among clinical dental undergraduates. A cross-sectional study was conducted among 127 dental undergraduates from Universiti Teknologi MARA (UiTM) Sungai Buloh, Malaysia. Overall the scores of DASS-21 shows the majority of dental undergraduates have no depression, anxiety and stress. This study also highlights the main factor affecting the dental undergraduates’ depression levels is the students’ educational environment. Future research on any correlation between depression levels and clinical performances is highly recommended for better understanding on this topic, and to provide a better educational environment at dental institutes in Malaysia. .
Performance Comparison of Basic Idea Sketches for Product Development between Manual Sketching and Computer Aided Design
Authors: Pijukkana Pracha , Rugwongwan Yanin
Published: November 2017

This research is a qualitative study on basic idea sketches between Manual Sketching and Computer Aided Design Modeling (CAD). The result of this research will be to improve or to lead to design idea transferring process; that presents the product sketch, which is more advantage in respond to the designers' mindset, and the result will be recommended as a guideline for instruction industrial design which will be suitable for all students with different aptitudes in the design processes.
A Taste of Spatial Character: Quality outdoor space in campus landscape leisure setting
Authors: Mt Akhir Norizan , Md Sakip Siti Rasidah , Abbas Mohamed Yusoff , Othman Noriah
Published: November 2017

The landscape is important in spatial development for campus space. Fundamentally, planting composition generates a character of the outdoor space. It could offer an enormous influence on visual quality, student performance and quality of life. This paper investigated the preferences and usage of outdoor campus space provided by universities during students’ leisure time. The data were collected through structured interviews at Research Universities in Malaysia. The results showed a relationship between preferences for landscape setting and utilisation index. Hence, this study suggested, the planting design is substantial to generate the space character which stimulates the use of outdoor campus space.
Participação popular na reforma constitucional: estudo comparado entre Brasil e Bolívia
Authors: De Souza Neyllon Rangel , De Lima Clara Pinheiro , Cyrillo da Silva Carolina Machado
Published: November 2017

RESUMO:

Entre as diversas nações que compõem o constitucionalismo sul-americano, no presente estudo, busca-se analisar os mecanismos de participação popular no âmbito do processo legislativo especial referente às emendas ao texto constitucional de dois países específicos, quais sejam, o Brasil e a Bolívia. A partir de um prisma eminentemente democrático, que outorga ao povo a legitimidade de exercício do poder constituinte derivado reformador, se almeja identificar aspectos convergentes ou discrepantes em ambos os sistemas, considerando nesse desiderato seus distintos marco-teórico filosóficos - no Brasil, o Neoconstitucionalismo e na Bolívia, o Novo Constitucionalismo Latino Americano - os quais implicam nos diversos elementos de expressão do desenvolvimento dos institutos em ambos os fenômenos doravante analisados

ABSTRACT:

Among the various nations that compose South American constitutionalism, the present study aims to analyze the mechanisms of popular participation in the special legislative process concerning the amendments to the constitutional text of two specific countries, namely Brazil and Bolivia. From an eminently democratic perspective, which grants to people the legitimacy to exercise the constituent power of reform, it is sought to identify convergent or discrepant aspects in both systems, considering in this desideratum its different philosophies and theoretical frameworks - in Brazil, neo-constitutionalism and in Bolivia, the New Latin American Constitutionalism - which imply in the various elements of development of institutes in both phenomena henceforth analyzed.
National Security and the Free Exercise Guarantee of Section 116: Time for a Judicial Interpretive Update
Authors: Babie Paul
Published: November 2017

This article, in three parts, suggests both why and how the courts ought to reconsider, and thereby update, the approach to the free exercise guarantee of s 116. First, having briefly outlined the current interpretation of the free exercise guarantee, it suggests the necessity for an update based upon the need for liberal constitutional democracies to provide what has been referred to as ‘“constitutional space” for investigation and pursuit of truth.’ Second, it proposes a ‘judicial interpretive update’ to the interpretation of s 116, outlining a two-stage approach which, in the first stage, sets the ambit of the right and, in the second, provides for a limitations standard by which to assess infringements of the right. Finally, the article concludes that the proposed update to s 116 ensures a robust protection for free exercise in its application to Commonwealth legislation and executive action.
Australia's Commonwealth Ombudsman 40 Years on—Achievements and Ten Challenges
Authors: Kirby Michael
Published: November 2017

The statutory office of Commonwealth Ombudsman was created in 1977, as part of the new federal administrative law. In this article, marking the 40th anniversary of the appointment of the first Commonwealth Ombudsman, Professor Jack Richardson, the author describes the early history, including the resistance in some quarters to the model adapted from Sweden. He describes the innovations of the first ten ombudsmen and the frustrations and difficulties they faced. He then identifies ten issues on the future of the office: (1) whether it should be directly linked to the Parliament; (2) whether it should be served by a select committee; (3) how its funding should be maintained; (4) protecting the Ombudsman title; (5) retaining ministerial support in circumstances of differences; (6) overcoming official resistance; (7) addressing judicial hostility; (8) embracing new technology and generic reform; (9) embracing self-criticism and human rights analysis; and (10) engaging with comparative and international outreach and innovation.
Biosorpsi ion merkuri menggunakan jamur pelapuk putih imobil [Biosorption of mercury ion using immobile white-rot fungi]
Authors: DIMAWARNITA Firda , PANJI Tri , MULYOPRAWIRO Suharyanto
Published: November 2017

Meningkatnya aktivitas pertambangan membawa dampak negatif bagi lingkungan apabila tidak dikelola dengan baik. Merebaknya kasus-kasus kerusakan lingkungan mulai dari yang kecil sampai ke tahap yang bersifat serius di Indonesia merupakan dampak dari terakumulasinya kerusakan dalam jangka waktu yang relatif lama. Limbah pertambangan masih mengandung logam berat, salah satunya Hg(II). Limbah tersebut berpotensi mencemari perairan dan lahan pertanian bila tidak ditangani dengan baik.  Usaha untuk mengatasi limbah tailing dan sekaligus memekatkan (recovery) logam di dalamnya dapat dilakukan dengan proses biosorpsi menggunakan mikroorganisme. Penelitian ini bertujuan menetapkan kemampuan biomassa Jamur Pelapuk Putih(JPP) yang diamobilisasi dengan tandan kosong kelapa sawit (TKKS) dalam mengabsorpsi logam berat Hg (II).  Hasil seleksi JPP berdasarkan laju pertumbuhan dalam media mengandung logam berat Hg (II) dan penyerapan logam berat telah diperoleh kandidat JPP unggul yaitu Omphalina sp. Dalam media PDB,  Omphalina sp. toleran terhadap Hg (II) sampai dengan konsentrasi 5 ppm. Biomassa Omphalina sp. yang diamobilisasi dengan TKKS mampu menurunkan hingga 84-96% logam berat Hg (II) pada pH 4,0 selama 60 menit. Kapasitas biosorpsi Omphalina sp. amobil maksimum (q max) untuk Hg (II) sebesar 0,1619 mg/g sehingga berpotensi untuk bio-konsentrasi logam berat.
STK11 gene mutations among patients with sporadic breast cancer
Authors: Antov Georgi , Krasteva Maria , Andonova Silvia , Savov Alexey , Angelova Svetla , Stoilov Lubomir , Toncheva Draga
Published: November 2017

Germline mutations affecting STK11 (LRG_319) are profoundly studied in
relation to Peutz-Jeghers syndrome, predisposing to the development of
various cancers at multiple sites. Though somatic mutations in STK11 are
found to be present in several cancers, limited data on its involvement in
sporadic breast cancer are available. The present study aims to evaluate the
frequency and spectrum of genetic alterations in STK11 in a group of
Bulgarian patients with sporadic breast cancer. A total of 73 tumor and 22
corresponding blood specimens derived from the patients, and 10 blood
samples from clinically healthy controls were analyzed. High Resolution
Melting analysis followed by Sanger sequencing and bioinformatic prediction
tools were utilized. Seven patients (9.58%) harbored STK11 alterations, only
two (2.74%) of which are exonic: one nonsense c.322A>T; p.K108X
(deleterious) and one missense c.440G>A; p.Arg147His (of unknown
significance). Two intronic variants were also observed: c.290+36G>T and
c. *16+18C>A (novel). To our knowledge the results represent the first data
indicating presence of STK11 alterations in patients with sporadic breast
cancer. The limited number of the detected deleterious mutations indicates
that mutational inactivation of the gene is a rare event and probably plays
a minor role in sporadic breast carcinogenesis.
Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers
Authors: Hasanova Saida , Akparov Zeynal , Mammadov Alamdar , Amirov Litfer , Babayeva Sevda , Nasibova Jale , Mukhtarova Zumrud , Shikhaliyeva Kamila , İzzatullayeva Vusala , Abbasov Mehraj
Published: November 2017

Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11
RAPD primers. In the study RAPD primers detected more polymorphism (98%)
than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR
and 0.85 for RAPD) for each of these marker systems. Cluster analysis
performed from both separate and combined data of RAPD and ISSR markers
using SPSS software package. Jaccard?s similarity coefficient for 62
chickpea genotypes was 0.65. Cluster analyses based on combined data
generated a dendrogram that separated genotypes into 11 clusters. Four
clusters contained only one genotype showing the genetic uniqueness of these
accessions. The studied chickpea collection has been proved to constitute a
rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing
between distantly related genotypes is expected to yield more vigorous
plants constituting much of the different traits contained in the two
parental lines.
Genetic diversity of characteristics in barley cultivars
Authors: Saroei Elahe , Cheghamirza Kianoosh , Zarei Leila
Published: November 2017

Knowledge of the genetic diversity is important to design appropriate
selection criteria in the breeding programs. In this study, the genetic
diversity of 42 Iranian and European barley cultivars was investigated with
morpho-agronomic traits including phenological, physiological, morphological
traits, grain yield and associated traits. Analysis of variance showed high
variability among cultivars. The European cultivars Panaka, Aiace and
Pariglia had the highest grain yield. The results of group comparisons
indicated that the European cultivars produced higher grain yield (500.57
g/m2) than the Iranian cultivars (445.50 g/m2), but larger genetic diversity
based on morpho-agronomic traits was observed among Iranian cultivars than
European cultivars. Correlation analysis revealed the high significant
correlations between grain yield with biological yield (0.92), straw yield
(0.77), and number of spike per square meter (0.67). Based on the factor
analysis, the six factors that justified 81.63 percent of the variations
were identified. The first factor having the largest eigenvalue was
identified as effective factor on the vegetative and reproductive growth. In
path analysis, biological yield had the greatest effect on grain yield
(0.906). Cluster analysis classified the cultivars in six groups and showed
that genetic variation based on the all studied traits among the barley
cultivars was not related to geographical location.
Variability of morphological characters among ornamental sunflower collection
Authors: Mladenovic Emina , Cvejic Sandra , Jocic Sinisa , Cukanovic Jelena , Jockovic Milan , Malidza Goran
Published: November 2017

The research describes the field comparison of 81 decorative sunflower
genotypes. In order to assess genetic diversity of sunflower genotypes the
studies were conducted in the field conditions during 2010-2015 at the
Institute of Field and Crops, Novi Sad, Serbia. The genetic diversity of
species Helianthus annuus L. has enabled the breeding work in the direction
of the decorating and plant landscaping. Depending on the qualitative and
quantitative characteristics, production of decorative sunflowers can be
divided into three directions. The first line is for the production of cut
flowers, the second one is for garden production and the third line is for
the production of pot plants. The direction of production dictates the main
breeding objectives, which may include: plant architecture, the color of ray
and disc flowers and duration of flowering. Investigation of the genetic
variability of ornamental sunflowers relies on quantitative traits of which
the greatest variability was observed in branching and plant height, which
are also the most important qualities for production. The quantitative
characteristics of decorative sunflowers have been examined on the basis of
81samples.
Assessment of molecular and phenotypic diversity among winter wheat cultivars
Authors: Petrovic Sonja , Maric Sonja , Cupic Tihomir , Rebekic A. , Rukavina Ivana
Published: November 2017

Comparing results of different genetic diversity estimates can be useful in
parental selection for plant breeders. Forty winter wheat cultivars, from
three Croatian breeding centres and four foreign countries, were used to
utilize and compare agronomic, morphologic and molecular based genetic
diversity estimates. Ten morphologic descriptors according to UPOV
guidelines and eight agronomic traits were used to establish phenotypic
data. Molecular data consisted of 26 SSR and four combinations of AFLP
markers, covering all three wheat genomes. Agro-morphologic data showed
variability especially regarding plant height (CV=18.44%), yield
(CV=22.02%), and ear emergence (range=8). Discriminant analysis confirmed
grouping among cultivars was mostly influenced by number of days to heading
and yield. The four AFLP primer combinations and 26 SSR markers yielded 108
polymorphic bands. The UPGMA based on phenotypic data, arranged cultivars in
four clusters, with one distinctive outlier, cultivar U1. The UPGMA based on
molecular data also arranged cultivars in four clusters, with one
distinctive outlier, cultivar Antonius. The similarities based on all four
genetic diversity estimates reflected, on average, the degree of relatedness
of cultivars used. No correlations between phenotypic and molecular data
were found implying that both types of data should be used for genetic
diversity estimates in order to cover wider variability between tested
cultivars.
Influence of GSTT1 and GSTM1 null genotypes on differentiated thyroid cancer risk and baseline and radioiodine induced cytogenetic damage in peripheral blood lymphocytes of patients
Authors: Milosevic-Djordjevic Olivera , Grujicic Darko , Radovic-Jakovljevic Marina , Marinkovic Dragoslav , Dimitrijevic Sladjana , Mihaljevic Olgica , Mijatovic-Teodorovic Ljiljana , Zivancevic-Simonovic Snezana
Published: November 2017

As it is known that genetic polymorphisms of glutathione S-transferases (GST)
have been associated with a variety of human diseases including cancer, we
have analyzed the impact of GSTT1 and GSTM1 null genotypes on the risk of
development of differentiated thyroid cancer (DTC) and cytogenetic changes
in peripheral blood lymphocytes of DTC patients before and after radioiodine
therapy. The polymorphism of GSTT1 and GSTM1 genes were genotyped using
multiplex polymerase chain reaction (PCR) and cytokinesis - block
micronucleus (MN) assay to assess cytogenetic changes. GSTT1 and GSTM1 null
were predominantly found in patients, but statistical significance was
observed only for GSTT1 null. The null genotypes increased risk of
development of DTC; GSTT1 null a 4.5 times (p < 0.05), GSTM1 null about 3
times but on the border of statistical significance (p = 0.057), while
combination of dual null genotypes almost 7 times (p < 0.05) increased risk.
No significant effects of the null genotypes as well as their interactions
with potential modifiers of MN (diagnose, age, gender and smoking habits)
were observed on both baseline and radioiodine-induced values of MN and
cytokinesis block proliferation index (CBPI) in DTC patients. Results
suggest that both GSTT1 and GSTM1 null genotypes increased risk for DTC but
to a greater extent GSTT1 null. Null genotypes of GSTT1 and GSTM1 did not
have potential to influence baseline and radioiodine-induced values of MN
and CBPI, so that absence of T1 and M1 isoenzymes did not cause increased
mutagen sensitivity of PBLs of DTC patients.
Evaluation of DGAT1-exon 8 K232A substitution in Gir and Kankrej (Bos indicus), Indian origin cattle and its association with milk production traits
Authors: Patel Jainikkumar , Chauhan Jenabhai
Published: November 2017

The aim of the present study was to screen the genotype profile of bovine
DGAT1 gene among Gir and Kankrej cattle in Gujarat, India. Total number of
200 Gir cattle and 100 Kankrej cattle were evaluated for CfrI-RFLP based
genotyping of DGAT1 gene. We observed that only two genotypes (KK and KA)
exist among the studied population with the alleles frequency of 0.925
(n=170) and 0.075 (n=30), respectively for K and A in Gir cattle as well as
0.895 (n=79) and 0.105 (n=21) respectively in Kankrej cattle. The overall
calculated allele frequency for K and A was 0.915 and 0.085, respectively in
both zebu (Bos indicus) cattle. Association of genotypes with milk
production traits revealed that KA had significant (P<0.05) effect on total
milk yield, as compared to KK in Gir and Kankrej cattle breed. Similarly, KK
genotype had significant (P<0.05) effect on fat percentage, as compared to
KA in Gir and Kankrej.
Assessment of sugar beet root yield by AMMI analysis
Authors: Ciric Mihajlo , Curcic Zivko , Mirosavljevic Milan , Marjanovic-Jeromela Ana , Jacimovic Goran , Prodanovic Slaven , Zivanovic Tomislav
Published: November 2017

Sugar beet cultivars have different responses in various environments, such
as different locations, years, mineral nutrition treatments or the
combination of these factors, due to genotype ? environment interaction.
Additive main effect and multiplicative interaction (AMMI) is one of the
most commonly used multivariate methods for analysis and visualization of
genotype ? environment interaction data. The main goals of the present study
were to (i) investigate the application of AMMI method in the analysis of
genotype ? fertilizer interaction in sugar beet, (ii) to assess genotype ?
fertilizer interaction, and (iii) to identify sugar beet cultivars with the
most stable response and high yield performance across different mineral
nutrition treatments. The trial with eight sugar beet cultivars was
conducted in two successive growing seasons at Rimski sancevi, Serbia. The
different levels of nitrogen, phosphorous and potassium fertilizers (0, 50,
100 and 150 kg ha-1) and their combinations represented specific
environments for testing genotype ? fertilizer interaction. Results from the
analysis of variance indicated that the fertilizer treatment, cultivars, and
their interaction significantly affected root yield variation in both
seasons. Results from our study suggest that AMMI model with two and three
first IPCA axes were recommended in 2014 and 2015, respectively. According
to AMMI 1 and AMMI 2 biplot, E14 and E15 were high yielding and among the
most stable treatments in both years. Among high yielding genotypes in 2014,
G4 and G8 stand out as the most stable, while in the following year G3 had
the lowest interaction score. AMMI analysis enabled identification of
specific associations between cultivars and different mineral nutrition
treatments, which was important for adjustment of fertilizer management for
each cultivar in order to achieve high root yield with decreased and more
rational fertilizer doses.
Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (GMP) content as revealed by size-exclusion high performance liquid chromatography (SE-HPLC)
Authors: Wu Jisu , Lu Xiaobin , Yu Zitong , Han Caixia , Li Xiaohui , Prodanovic Slaven , Yan Yueming
Published: November 2017

Gluten macropolymers (GMPs), formed by seed storage proteins glutenins and
gliadins through intermolecular disulfide bond, confer dough viscoelasticity
andwheat processing quality. Glutenins consist of highand low molecular weight
glutenin subunits (HMW-GS, LMW-GS)encoded by Glu-1 and Glu-3 loci,
respectively. The allelic variations at both loci have important effects on
GMP content and breadmaking quality. In this study, GMP extraction and
size-exclusion high performance liquid chromatography (SE-HPLC) separation
were optimized, and then applied to investigate the effects of
Glu-1 and Glu-3 allelic variations on GMP content using different wheat
genotypes, chromosome substitution lines and near-isogenic lines (NILs). The
results showed that the optimized GMP extraction and SE-HPLC protocol could
obtain a reproducible separation and reliable quantitation of GMP content
with small samples. The allelic variations at Glu-1 and Glu-3 were closely
related to GMP content. Particularly, Glu-D1 dencoded 1Dx5+1Dy10 subunits,
Glu-A3a and Glu-B3 hencoded an abundant LMW-B subunit respectively had positive
effects on GMP content and bread making quality. The introgress of HMW-GS and
LMW-GS into bread wheat from related genomes could significantly increase GMP
content, indicating that wheat related species has potential gene resources
for breadmaking quality improvement. Our results demonstrated that SE-HPLC
could serve as an effective tool for rapid separation and quantitation of
GMPs and had potential application for gluten quality screening in the early
generations during wheat quality improvement program.
Molecular characterization of some selected persimmon genotypes and cultivars by srap and ssr markers
Authors: Pinar Hasan , Yildiz Ercan , Kaplankiran Mustafa , Toplu Celil , Unlu Mustafa , Serce Sedat , Ercisli Sezai
Published: November 2017

In this study, SRAP and SSR markers were employed to determine genetic
relationships among 42 persimmon genotypes (Diospyros kaki Thunb) obtained
from Hatay province and 3 persimmon cultivars, 2 of which belong to
Diospyros kaki Thunb and one belongs to Diospyros oleifera Cheng. Genetic
relationships were determined by using a total of 29 molecular DNA primers
(SRAP and SSR). Of these primers, 21 SRAP primer combinations produced a
total of 107 bands and 77.6% of them were polymorphic; 8 SSR primers
produced 26 polymorphic bands with an average polymorphism ratio of 84.6%.
The SRAP and SSR markers produced 4.6 bands as average and the number of
bands produced per marker was calculated as 3.6. The lowest similarity was
observed between MK-113 (Diospyros oleifera Cheng) and the other genotypes
all belongs to Diospyros kaki Thunb (with similarity ratios of 0.41-0.69 for
SRAP primers, between 0.25-0.67 for SSR primers). The genotypes/cultivars
belongs to Diospyros kaki had similarity ratio between 0.98-1.00 according
to SRAP and SSR markers. This synonym or similarity could be results of
clonal propagation rather than autogamy.
Genetic and multivariate phenotypic analyses of some selection indices in pistachio (Pistacia vera L.) cultivars under drought stress conditions
Authors: Hasheminasab Hojjat , Assad Mohammad
Published: November 2017

Heritability and Genetic Gain are commonly used by plant breeders to estimate
the accuracy of a selection index and used for measuring the response to
selection. The aim of the present study was the genetic and phenotypic
analyses of some physiological traits to identify the most reliable of them
for selective breeding of pistachio cultivars under drought stress
condition. Nineteen Pistachio (Pistacia vera L.) cultivars with wide range
of tolerance to drought stress were collected from across the orchards of
Rafsanjan (Iran?s center of pistachio cultivation) and were used in
randomized complete block design with three replications under two
environmental conditions (normal and water stress) in 2011-2012. The results
of genetic analysis showed that high magnitude of phenotypic and genotypic
coefficient of variation along with broad sense heritability and genetic
gain were estimated in relative water protection (RWP), excised leaf water
loss (ELWL), relative water content (RWC) and relative water loss (RWL)
under drought stress condition, indicating that the inheritance of these
traits can be mainly controlled by additive gene effects followed by
reflecting the possibility of effective selection for genetic improvement of
these traits. Path analysis revealed that RWP had the highest direct and RWL
and WRC indirect effects on YSI. Principal component analysis indicated that
selected indices were reliable for classification and identification of
drought-tolerant pistachio cultivars and identified RWP, RWC and WRC as the
best indicators for screening drought tolerant genotypes.
Genetic testing for Bardet-Biedl syndrome
Authors: Abeshi Andi , Fanelli Francesca , Beccari Tommaso , Dundar Munis , D’Esposito Fabiana , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of genetic testing for Bardet- Biedl syndrome (BBS). The disease has autosomal recessive inheritance, a prevalence varying from one in 13 500 to one in 160 000, and is caused by mutations in the ARL6, BBIP1, BBS1, BBS2, BBS4, BBS5, BBS7, BBS9, BBS10, BBS12, CEP290, IFT172, IFT27, LZTFL1, MKKS, MKS1, NPHP1, SDCCAG8, TRIM32, TTC8 and WDPCP genes. The clinical diagnosis of BBS is based on four primary features or three primary features plus two secondary features. The genetic test is useful for confirming diagnosis, and for differential diagnosis, couple risk assessment and access to clinical trials.
Genetic testing for choroideremia
Authors: Abeshi Andi , Zulian Alessandra , Beccari Tommaso , Dundar Munis , Viola Francesco , Garoli Elena , Colombo Leonardo , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of the genetic test for choroideremia (CHM). CHM is an inherited X-linked recessive disorder associated with variations in the CHM gene. The overall prevalence of CHM varies from 1 in 50 000 to 1 in 100 000. Clinical diagnosis is based on clinical findings, ophthalmological examination, visual field, fundus autofluorescence, optical coherence tomography and electroretinography. The genetic test is useful for confirming diagnosis and for differential diagnosis, couple risk assessment and access to clinical trials.
Genetic testing for cone rod dystrophies
Authors: Abeshi Andi , Zulian Alessandra , Beccari Tommaso , Dundar Munis , Ziccardi Lucia , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of the genetic test for cone rod dystrophies (CORDs). CORDs are caused by variations in the ABCA4, ADAM9, AIPL1, C8orf37, CACNA1F, CACNA2D4, CDHR1, CNGA3, CRX, DRAM2, GUCA1A, GUCY2D, HRG4, KCNV2, PDE6C, PITPNM3, POC1B, PROM1, PRPH2, RAB28, RAX2, RIMS1, RPGRIP1, RPGR SEMA4A, TTLL5 genes, with an overall prevalence of 1 per 40 000. Most genes have autosomal recessive inheritance; the others have autosomal dominant or X-linked recessive transmission. Clinical diagnosis is based on clinical findings, color vision testing, ophthalmological examination and electroretinography. The genetic test is useful for confirming diagnosis, and for differential diagnosis, couple risk assessment and access to clinical trials.
Genetic testing for gyrate atrophy of the choroid and retina
Authors: Abeshi Andi , Fanelli Francesca , Beccari Tommaso , Dundar Munis , Benedetto Falsini , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of genetic testing for gyrate atrophy of the choroid and retina (GACR). GACR is inherited in an autosomal recessive manner, and has a prevalence of 1/50000 in Finland. In the international literature there are approximately 200 biochemically confirmed cases. GACR is caused by mutations in the OAT gene. Clinical diagnosis involves ophthalmological examination, electrophysiological testing (electroretinography - ERG), coherence tomography and assay of ornithine levels in body fluids. The genetic test is useful for confirming diagnosis, as well as for differential diagnosis, couple risk assessment and access to clinical trials.
Genetic testing for infantile nystagmus
Authors: Abeshi Andi , Coppola Pamela , Beccari Tommaso , Dundar Munis , Colombo Leonardo , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of genetic testing for infantile nystagmus (IN). Forms of IN associated with variations in CACNA1F, FRMD7 and GPR143 genes have X-linked recessive inheritance, whereas variations in SLC38A8, TYR and TYRP1 genes have an autosomal recessive inheritance and variations in COL11A1, CRYBA1 and PAX6 genes have an autosomal dominant inheritance. The prevalence of all forms of IN is estimated to be 1 in 5000. Clinical diagnosis is based on clinical findings, age of onset, family history, ophthalmological examination, fundoscopy, electroretinography, optical coherence tomography, slit lamp examination and visual evoked potentials. The genetic test is useful for confirming diagnosis, and for differential diagnosis, couple risk assessment and access to clinical trials.
Genetic testing for Norrie disease
Authors: Abeshi Andi , Marinelli Carla , Beccari Tommaso , Dundar Munis , Ziccardi Lucia , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of genetic testing for Norrie disease. The disease is caused by variations in the NDP gene. Its prevalence is currently unknown. Inheritance is X-linked recessive. Clinical diagnosis is based on clinical findings, color vision testing, optical coherence tomography, ophthalmological examination and electroretinography. The genetic test is useful for confirming diagnosis, and for differential diagnosis, couple risk assessment and access to clinical trials.
Genetic testing for pattern dystrophies
Authors: Abeshi Andi , Coppola Pamela , Beccari Tommaso , Dundar Munis , Di Nicola Maura , Viola Francesco , Colombo Leonardo , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of the genetic test for pattern dystrophies. Pattern dystrophies are mostly inherited in an autosomal dominant manner (autosomal recessive transmission is rare). The overall prevalence is currently unknown. Pattern dystrophies are caused by variations in the BEST1, IMPG1, IMPG2, OTX2, PRPH2 and CTNNA1 genes. Clinical diagnosis is based on clinical findings, ophthalmological examination, optical coherence tomography, electrooculography and electroretinography. The genetic test is useful for confirming diagnosis and for differential diagnosis, couple risk assessment and access to clinical trials.
Genetic testing for retinitis punctata albescens/fundus albipunctatus
Authors: Abeshi Andi , Coppola Pamela , Beccari Tommaso , Dundar Munis , D’Esposito Fabiana , Bertelli Matteo
Published: November 2017

We studied the scientific literature and disease guidelines in order to summarize the clinical utility of genetic testing for retinitis punctata albescens/fundus albipunctatus (RPA/FA). RPA and FA are reported to have autosomal dominant or autosomal recessive inheritance and are associated with variations in the PRPH2, RHO, RLBP1 and RDH5 genes. There is insufficient data to establish their prevalence. Clinical diagnosis is based on clinical findings, ophthalmological examination, optical coherence tomography, visual field testing and undetectable or severely reduced electroretinogram amplitudes. The genetic test is useful for confirming diagnosis, and for differential diagnosis, couple risk assessment and access to clinical trials.