bluuebunny/crossref_metadata_2025 huggingface dataset:
FUS Microphase Separation: Regulation by Nucleic Acid Polymers and DNA Repair Proteins
Authors: Sukhanova Maria V. , Anarbaev Rashid O. , Maltseva Ekaterina A. , Pastré David , Lavrik Olga I.
Published: October 2022

Fused in sarcoma (FUS) is involved in the regulation of RNA and DNA metabolism. FUS participates in the formation of biomolecular condensates driven by phase transition. FUS is prone to self-aggregation and tends to undergo phase transition both with or without nucleic acid polymers. Using dynamic light scattering and fluorescence microscopy, we examined the formation of FUS high-order structures or FUS-rich microphases induced by the presence of RNA, poly(ADP-ribose), ssDNA, or dsDNA and evaluated effects of some nucleic-acid-binding proteins on the phase behavior of FUS–nucleic acid systems. Formation and stability of FUS-rich microphases only partially correlated with FUS’s affinity for a nucleic acid polymer. Some proteins—which directly interact with PAR, RNA, ssDNA, and dsDNA and are possible components of FUS-enriched cellular condensates—disrupted the nucleic-acid-induced assembly of FUS-rich microphases. We found that XRCC1, a DNA repair factor, underwent a microphase separation and formed own microdroplets and coassemblies with FUS in the presence of poly(ADP-ribose). These results probably indicated an important role of nucleic-acid-binding proteins in the regulation of FUS-dependent formation of condensates and imply the possibility of the formation of XRCC1-dependent phase-separated condensates in the cell.
11H-Benzo[4,5]imidazo[1,2-a]indol-11-one as a New Precursor of Azomethine Ylides: 1,3-Dipolar Cycloaddition Reactions with Cyclopropenes and Maleimides
Authors: Filatov Alexander S. , Pronina Yulia A. , Selivanov Stanislav I. , Shmakov Stanislav V. , Uspenski Anton A. , Boitsov Vitali M. , Stepakov Alexander V.
Published: October 2022

The possibility of generating azomethine ylides from 11H-benzo[4,5]imidazo[1,2-a]indol-11-one and amino acids is shown for the first time. Based on the cycloaddition reactions of these azomethine ylides with cyclopropenes and maleimides, cyclopropa[a]pyrrolizines, 3-azabicyclo[3.1.0]hexanes, and pyrrolo[3,4-a]pyrrolizines spiro-fused with a benzo[4,5]imidazo[1,2-a]indole fragment were synthesized. Spirocyclic compounds were obtained in moderate to good yields, albeit with poor diastereoselectivity. Density functional theory calculations were performed to obtain an insight into the mechanism of the 1,3-dipolar cycloaddition of 11H-benzo[4,5]imidazo[1,2-a]indol-11-one-derived azomethine ylides to cyclopropenes. The cytotoxic activity of some of the obtained cycloadducts against the human erythroleukemia (K562) cell line was evaluated in vitro by MTS-assay.
Chitosan/Lactic Acid Systems: Liquid Crystalline Behavior, Rheological Properties, and Riboflavin Release In Vitro
Authors: Selivanova Natalia M. , Galeeva Aliya I. , Galyametdinov Yuriy G.
Published: October 2022

Chitosan or its derivatives exhibit lyotropic liquid crystalline mesophases under certain conditions due to its semi-rigid structures. This work describes the development of chitosan-based biocompatible systems that include new components: lactic acid and non-ionic surfactants. Polarized optical microscopy studies revealed that these systems are capable of forming gels or lyotropic liquid crystals (LLCs) in a certain range of chitosan and lactic acid concentrations. According to the viscosity studies, the rheological flow of the LLCs can be accurately described by the Casson flow model. The intermolecular interactions of the LLC components were studied by FTIR spectroscopy. According to the FTIR data, hydrogen bonding is supposed to be responsible for the formation of the LLCs. In the studied systems, this LLC complex exists as the [ChitH+·CH3-CH(OH)-COO−] ion pair. The studied gel and LLCs were shown to possess the most prolonged release capabilities for riboflavin among similar binary LLC systems. The supramolecular organization and rheological characteristics of the studied chitosan-based systems were found to affect the release of riboflavin.
Genome-Wide Association Mapping Identifies New Candidate Genes for Cold Stress and Chilling Acclimation at Seedling Stage in Rice (Oryza sativa L.)
Authors: Li Jianguo , Khatab Ahmed Adel , Hu Lihua , Zhao Liyan , Yang Jiangyi , Wang Lingqiang , Xie Guosheng
Published: October 2022

Rice (Oryza sativa L.) is a chilling-sensitive staple food crop, and thus, low temperature significantly affects rice growth and yield. Many studies have focused on the cold shock of rice although chilling acclimation is more likely to happen in the field. In this paper, a genome-wide association study (GWAS) was used to identify the genes that participated in cold stress and chilling accumulation. A total of 235 significantly associated single-nucleotide polymorphisms (SNPs) were identified. Among them, we detected 120 and 88 SNPs for the relative shoot fresh weight under cold stress and chilling acclimation, respectively. Furthermore, 11 and 12 quantitative trait loci (QTLs) were identified for cold stress and chilling acclimation, respectively, by integrating the co-localized SNPs. Interestingly, we identified 10 and 15 candidate genes in 11 and 12 QTLs involved in cold stress and chilling acclimation, respectively, and two new candidate genes (LOC_Os01g62410, LOC_Os12g24490) were obviously up-regulated under chilling acclimation. Furthermore, OsMYB3R-2 (LOC_Os01g62410) that encodes a R1R2R3 MYB gene was associated with cold tolerance, while a new C3HC4-type zinc finger protein-encoding gene LOC_Os12g24490 was found to function as a putative E3 ubiquitin-protein ligase in rice. Moreover, haplotype, distribution, and Wright’s fixation index (FST) of both genes showed that haplotype 3 of LOC_Os12g24490 is more stable in chilling acclimation, and the SNP (A > T) showed a difference in latitudinal distribution. FST analysis of SNPs in OsMYB3R-2 (LOC_Os01g62410) and LOC_Os12g24490 indicated that several SNPs were under selection in rice indica and japonica subspecies. This study provided new candidate genes in genetic improvement of chilling acclimation response in rice.
Synergistic Effects of Silicon and Preservative on Promoting Postharvest Performance of Cut Flowers of Peony (Paeonia lactiflora Pall.)
Authors: Song Jinnan , Yang Jingli , Jeong Byoung Ryong
Published: October 2022

As a commercial high-grade cut flower, the marketability of herbaceous peony (Paeonia lactiflora Pall.) is limited by its short vase life in water. Si (silicon) is an alternative to improve the postharvest life of cut flowers. However, the effects of the combined application of Si and preservatives on the postharvest performance of cut peony flowers are unknown. In this study, the effects of a Si application and a preservative alone and collegial on the longevity of the vase life, water loss, antioxidant defense system, and stock carbohydrates level of cut flowers of three peony cultivars were investigated. It was observed that Si effectively prolonged the vase life, while the preservative alone, to a lesser extent, but markedly induced an early flowering and a greater flower diameter (flower open degree). The simultaneous use of Si and the preservatives not only showed larger flowers, but also improved the postharvest performance as characterized by an extended vase life and delayed the water loss. In addition, the Si supplementation dramatically intensified the antioxidant defense system (ameliorated antioxidant enzymes and alleviated ROS accumulation) in petals but did not increase the stock carbohydrates (starch and soluble sugars) levels, as compared to the treatment with the preservative alone. We show that a Si supplementation to a preservative is highly recommended for a large-scale use to promote the postharvest performance and competitiveness of marketed cut flowers.
Mitochondrial Epilepsy, a Challenge for Neurologists
Authors: Lopriore Piervito , Gomes Fábio , Montano Vincenzo , Siciliano Gabriele , Mancuso Michelangelo
Published: October 2022

Primary mitochondrial diseases are relatively common inborn errors of energy metabolism, with a combined prevalence of 1 in 4300. These disorders typically affect tissues with high energy requirements, including the brain. Epilepsy affects >1% of the worldwide population, making it one of the most common neurological illnesses; it may be the presenting feature of a mitochondrial disease, but is often part of a multisystem clinical presentation. The major genetic causes of mitochondrial epilepsy are mutations in mitochondrial DNA and in the nuclear-encoded gene POLG. Treatment of mitochondrial epilepsy may be challenging, often representing a poor prognostic feature. This narrative review will cover the most recent advances in the field of mitochondrial epilepsy, from pathophysiology and genetic etiologies to phenotype and treatment options.
Aspirin Inhibits the Inflammatory Response of Protease-Activated Receptor 1 in Pregnancy Neutrophils: Implications for Treating Women with Preeclampsia
Authors: Walsh Scott W. , Al Dulaimi Marwah , Strauss Jerome F.
Published: October 2022

Neutrophils expressing cyclooxygenase-2 (COX-2) extensively infiltrate maternal blood vessels in preeclampsia, associated with vascular inflammation. Because pregnancy neutrophils also express protease-activated receptor 1 (PAR-1, F2R thrombin receptor), which they do not in non-pregnant subjects, they can be activated by proteases. We tested the hypothesis that aspirin at a dose sufficient to inhibit COX-2 would reduce inflammatory responses in preeclampsia neutrophils. Neutrophils were isolated from normal pregnant and preeclamptic women at approximately 30 weeks’ gestation. Normal pregnancy neutrophils were treated with elastase, a protease elevated in preeclampsia, or elastase plus aspirin to inhibit COX-2, or elastase plus pinane thromboxane, a biologically active structural analog of thromboxane and a thromboxane synthase inhibitor. Preeclamptic pregnancy neutrophils were treated with the same doses of aspirin or pinane thromboxane. Confocal microscopy with immunofluorescence staining was used to determine the cellular localization of the p65 subunit of nuclear factor-kappa B (NF-κB) and media concentrations of thromboxane were measured to evaluate the inflammatory response. In untreated neutrophils of normal pregnant women, p65 was localized to the cytosol. Upon stimulation with elastase, p65 translocated from the cytosol to the nucleus coincident with increased thromboxane production. When neutrophils were co-treated with aspirin or pinane thromboxane, elastase was not able to cause nuclear translocation of p65 or increase thromboxane. In untreated neutrophils of preeclamptic women, the p65 subunit was present in the nucleus and thromboxane production was elevated, but when preeclamptic neutrophils were treated with aspirin or pinane thromboxane, p65 was cleared from the nucleus and returned to the cytosol along with decreased thromboxane production. These findings suggest that COX-2 is a downstream mediator of PAR-1 and demonstrate that PAR-1- mediated inflammation can be inhibited by aspirin. Given the extensive and ubiquitous expression of PAR-1 and COX-2 in preeclamptic women, consideration should be given to treating women with preeclampsia using a dose of aspirin sufficient to inhibit COX-2.
Construction of Highly Active Zn3In2S6 (110)/g-C3N4 System by Low Temperature Solvothermal for Efficient Degradation of Tetracycline under Visible Light
Authors: Huo Haohao , Li Yuzhen , Wang Shaojie , Tan Siyang , Li Xin , Yi Siyuan , Gao Lizhen
Published: October 2022

Herein, Zn3In2S6 photocatalyst with (110) exposed facet was prepared by low temperature solvothermal method. On this basis, a highly efficient binary Zn3In2S6/g-C3N4 was obtained by low temperature solvothermal method and applied to the degradation of tetracycline (TC). The samples of the preparation were characterized by X-ray diffraction, scanning electron microscope, transmission electron microscope, UV–vis diffuse reflection spectroscopy, and photoluminescence spectroscopy. Furthermore, the degradation performance of photocatalysts on TC was investigated under different experimental conditions. Finally, the mechanism of Zn3In2S6/g-C3N4 composite material degrading TC is discussed. The results show that Zn3In2S6 and Zn3In2S6/g-C3N4 photocatalysts with excellent performance could be successfully prepared at lower temperature. The Zn3In2S6/g-C3N4 heterojunction photocatalyst could significantly improve the photocatalytic activity compared with g-C3N4. After 150 min of illumination, the efficiency of 80%Zn3In2S6/g-C3N4 to degrade TC was 1.35 times that of g-C3N4. The improvement of photocatalytic activity was due to the formation of Zn3In2S6/g-C3N4 heterojunction, which promoted the transfer of photogenerated electron–holes. The cycle experiment test confirmed that Zn3In2S6/g-C3N4 composite material had excellent stability. The free radical capture experiment showed that ·O2− was the primary active material. This study provides a new strategy for the preparation of photocatalysts with excellent performance at low temperature.
Knockdown of CLAUDIN-6 Inhibited Apoptosis and Induced Proliferation of Bovine Cumulus Cells
Authors: Wang Wanjie , Zou Huiying , Chen Nanzhu , Tian Yaqing , Hao Haisheng , Pang Yunwei , Zhao Xueming , Zhu Huabin , Yu Dawei , Du Weihua
Published: October 2022

This study aims to investigate the effects of CLAUDIN-6 (CLDN6) on cell apoptosis and proliferation of bovine cumulus cells (CCs). Immunofluorescence staining was used to localize CLDN6 protein in CCs. Three pairs of siRNA targeting CLDN6 and one pair of siRNA universal negative sequence as control were transfected into bovine CCs. Then, the effective siRNA was screened by real-time quantitative PCR (RT-qPCR) and Western blotting. The mRNA expression levels of apoptosis related genes (CASPASE-3, BAX and BCL-2) and proliferation related genes (PCNA, CDC42 and CCND2) were evaluated by RT-qPCR in CCs with CLDN6 knockdown. Cell proliferation, apoptosis and cell cycle were detected by flow cytometry with CCK-8 staining, Annexin V-FITC staining and propidium iodide staining, respectively. Results showed that the CLDN6 gene was expressed in bovine CCs and the protein was localized in cell membranes and cytoplasms. After CLDN6 was knocked down in CCs, the cell apoptosis rate significantly decreased and the pro-apoptotic genes BAX and CASPASE-3 were down-regulated significantly, whereas the anti-apoptotic gene BCL-2 was markedly up-regulated (p < 0.05). Additionally, CLDN6 knockdown significantly enhanced cell proliferation of CCs at 72 h after siRNA transfection. The mRNA levels of proliferation-related genes PCNA, CCND2 and CDC42 increased obviously in CCs with CLDN6 knockdown (p < 0.05). After CLDN6 was down-regulated, the percentage of CCs at S phase was significantly increased (p < 0.05). However, there was no remarkable difference in the percentages of cells at the G0/G1 phase and G2/M phase between CCs with or without CLDN6 knockdown (p > 0.05). Therefore, the expression of CLDN6 and its effects on cell proliferation, apoptosis and cell cycle of bovine CCs were first studied. CLDN6 low expression inhibited cell apoptosis, induced cell proliferation and cell cycle arrest of bovine CCs.
Blockage of KHSRP-NLRP3 by MCC950 Can Reverse the Effect of Manganese-Induced Neuroinflammation in N2a Cells and Rat Brain
Authors: Singh Sharad , Shaikh Ibrahim Ahmed , More Sunil S. , Mahnashi Mater H. , Almohaimeed Hailah M. , El-Sherbiny Mohamed , Ghoneim Mohammed M. , Umar Ahmad , Soni Harshit Kumar , Agrawal Himanshu , Mannasaheb Basheer Ahmed , Khan Aejaz Abdullatif , Muddapur Uday M. , Iqubal S. M. Shakeel
Published: October 2022

Manganese neurotoxicity has been reported to cause a neurodegenerative disease known as parkinsonism. Previous reports have shown that the expression of the KH-type splicing regulatory protein (KHSRP), a nucleic acid-binding protein, and NLRP3 is increased upon Mn exposure. However, the relation between these two during Mn toxicity has not been fully deduced. The mouse neuroblastoma (N2a) and SD rats are treated with LPS and MnCl2 to evaluate the expression of KHSRP and NLRP3. Further, the effect of the NLRP3 inhibitor MCC950 is checked on the expression of NLRP3, KHSRP and pro-inflammatory markers (TNFα, IL-18 and IL-1β) as well as the caspase-1 enzyme. Our results demonstrated an increment in NLRP3 and KHSRP expression post-MnCl2 exposure in N2a cells and rat brain, while on the other hand with LPS exposure only NLRP3 expression levels were elevated and KHSRP was found to be unaffected. An increased expression of KHSRP, NLRP3, pro-inflammatory markers and the caspase-1 enzyme was observed to be inhibited with MCC950 treatment in MnCl2-exposed cells and rats. Manganese exposure induces NLRP3 and KHSRP expression to induce neuroinflammation, suggesting a correlation between both which functions in toxicity-related pathways. Furthermore, MCC950 treatment reversed the role of KHSRP from anti-inflammatory to pro-inflammatory.
Astroglial Connexins Inactivation Increases Relapse of Depressive-like Phenotype after Antidepressant Withdrawal
Authors: Portal Benjamin , Vasile Flora , Zapata Jonathan , Lejards Camille , Ait Tayeb Abd El Kader , Colle Romain , Verstuyft Céline , Corruble Emmanuelle , Rouach Nathalie , Guiard Bruno P.
Published: October 2022

Studies suggest that astrocytic connexins (Cx) have an important role in the regulation of high brain functions through their ability to establish fine-tuned communication with neurons within the tripartite synapse. In light of these properties, growing evidence suggests a role of Cx in psychiatric disorders such as major depression but also in the therapeutic activity of antidepressant drugs. However, the real impact of Cx on treatment response and the underlying neurobiological mechanisms remain yet to be clarified. On this ground, the present study was designed to evaluate the functional activity of Cx in a mouse model of depression based on chronic corticosterone exposure and to determine to which extent their pharmacological inactivation influences the antidepressant-like activity of venlafaxine (VENLA). On the one hand, our results indicate that depressed mice have impaired Cx-based gap-junction and hemichannel activities. On the other hand, while VENLA exerts robust antidepressant-like activity in depressed mice; this effect is abolished by the pharmacological inhibition of Cx with carbenoxolone (CBX). Interestingly, the combination of VENLA and CBX is also associated with a higher rate of relapse after treatment withdrawal. To our knowledge, this study is one of the first to develop a model of relapse, and our results reveal that Cx-mediated dynamic neuroglial interactions play a critical role in the efficacy of monoaminergic antidepressant drugs, thus providing new targets for the treatment of depression.
Ex Vivo Treatment with Allogenic Mesenchymal Stem Cells of a Healthy Donor on Peripheral Blood Mononuclear Cells of Patients with Severe Alopecia Areata: Targeting Dysregulated T Cells and the Acquisition of Immunotolerance
Authors: Kim Jung-Eun , Lee Yu-Jin , Lee Kyung-Jae , Park Song-Hee , Kang Hoon
Published: October 2022

Alopecia areata (AA) is an autoimmune condition related to the collapse of the immune privilege of hair follicles. Certain AA populations present severe clinical manifestations, such as total scalp hair or body hair loss and a treatment refractory property. The aim of this study was to assess the effects of allogenic human mesenchymal stem cells (hMSCs) from healthy donors on the peripheral blood mononuclear cells (PBMCs) of severe AA patients, with a focus on the change in the cell fraction of Th1, Th17, and Treg cells and immunomodulatory functions. PBMCs of 10 AA patients and eight healthy controls were collected. Levels of Th17, Th1, and Treg subsets were determined via flow cytometry at baseline, activation status, and after co-culturing with hMSCs. All participants were severe AA patients with SALT > 50 and with a long disease duration. While the baseline Th1 and Treg levels of AA patients were comparable to those of healthy controls, their Th17 levels were significantly lower than those of the controls. When stimulated, the levels of CD4+IFN-γ+ T cells of the AA patients rose sharply compared to the baseline, which was not the case in those of healthy controls. The cell fraction of CD4+Foxp3+ regulatory T cells also abruptly increased in AA patients only. Co-culturing with allogenic hMSCs in activated AA PBMCs slightly suppressed the activation levels of CD4+INF-γ+ T cells, whereas it significantly induced the differentiation of CD4+Foxp3+ regulatory T cells. However, these changes were not prominent in the PBMCs of health controls. To examine the pathomechanisms, PBMCs of healthy donors were treated with IFN-γ to induce AA-like environment and then treated with allogenic grants and compared with ruxolitinib as a positive treatment control. hMSC treatment was shown to significantly inhibit the mRNA levels of proinflammatory cytokines, such as IFN-γ, TNF-α, IL-1α, IL-2R, IL-15, and IL-18, and chemokines, such as CCR7 and CCR10, in IFN-treated PBMCs. Interestingly, hMSCs suppressed the activation of JAK/STAT signaling by IFN in PBMCs with an effect that was comparable to that of ruxolitinib. Furthermore, the hMSC treatment showed stronger efficacy in inducing Foxp3, IL-10, and TGF-β mRNA transcription than ruxolitinib in IFN-treated PBMCs. This study suggests that allogenic hMSC treatments have therapeutic potential to induce immune tolerance and anti-inflammatory effects in severe AA patients.
Molecular Mechanisms Linking Inflammation to Autoimmunity in Sjögren’s Syndrome: Identification of New Targets
Authors: Sisto Margherita , Ribatti Domenico , Lisi Sabrina
Published: October 2022

Sjögren’s syndrome (SS) is a systemic autoimmune rheumatic disorder characterized by the lymphocytic infiltration of exocrine glands and the production of autoantibodies to self-antigens. The involvement of the exocrine glands drives the pathognomonic manifestations of dry eyes (keratoconjunctivitis sicca) and dry mouth (xerostomia) that define sicca syndrome. To date, the molecular mechanisms mediating pathological salivary gland dysfunction in SS remain to be elucidated, despite extensive studies investigating the underlying cause of this disease, hampering the development of novel therapeutic strategies. Many researchers have identified a multifactorial pathogenesis of SS, including environmental, genetic, neuroendocrine, and immune factors. In this review, we explore the latest developments in understanding the molecular mechanisms involved in the pathogenesis of SS, which have attracted increasing interest in recent years.
Unraveling the Biosynthesis of Carvacrol in Different Tissues of Origanum vulgare
Authors: Hao Yuanpeng , Guo Xiaoqi , Yang Rui , Yan Yihao , Sun Meiyu , Li Hui , Bai Hongtong , Cui Hongxia , Li Jingyi , Shi Lei
Published: October 2022

Origanum vulgare, belonging to the Lamiaceae family, is a principal culinary herb used worldwide which possesses great antioxidant and antibacterial properties corresponding to various volatile organic components (VOCs). However, the metabolite profiles and underlying biosynthesis mechanisms of elaborate tissues (stems, leaves, bracts, sepals, petals) of Origanum vulgare have seldom been reported. Here, solid-phase microextraction–gas chromatography/mass spectrometry results showed that Origanum vulgare ‘Hot and Spicy’ (O. vulgare ‘HS’) was extremely rich in carvacrol and had the tissue dependence characteristic. Moreover, a full-length transcriptome analysis revealed carvacrol biosynthesis and its tissue-specific expression patterns of ‘upstream’ MVA/MEP pathway genes and ‘downstream’ modifier genes of TPSs, CYPs, and SDRs. Furthermore, the systems biology method of modular organization analysis was applied to cluster 16,341 differently expressed genes into nine modules and to identify significant carvacrol- and peltate glandular trichome-correlated modules. In terms of these positive and negative modules, weighted gene co-expression network analysis results showed that carvacrol biosynthetic pathway genes are highly co-expressed with TF genes, such as ZIPs and bHLHs, indicating their involvement in regulating the biosynthesis of carvacrol. Our findings shed light on the tissue specificity of VOC accumulation in O. vulgare ‘HS’ and identified key candidate genes for carvacrol biosynthesis, which would allow metabolic engineering and breeding of Origanum cultivars.
Size–Number and Shape Distribution of Buried Seeds in Soil in a Field Not Cultivated for More Than 10 Years
Authors: Dias Luís Silva
Published: October 2022

Seeds act as reserves for plant dispersion in time and their burial in soil plays an essential role in preventing or reducing losses. Two hypotheses regarding the depth distribution of seeds in soil were investigated. One states that the burial of small seeds is restricted to shallower depths than large seeds. The other states that seed shape is important to burial. The fraction of seeds located at depths allowing viable non-photosynthetic growth was also investigated in relation to size and shape. Cores of 20 cm depth were taken from soil with an auger, divided in eight fractions of equal length and sorted through a series of ten sieves, and viable spherical and non-spherical seeds were separately counted. Burial was evaluated by the symmetry of depth–number distributions fitted by Weibull equations. The maximum depth for successful germination and emergence was calculated by combining Weibull equations with published or original material on the relationship between the volume and mass of seeds, and the maximum elongation of hypocotyls in soil. The burial of smaller seeds was found to be restricted to shallower depths, but in larger seeds, size itself appeared to be an unsurmountable barrier to burial. Smaller spherical seeds buried at shallower depths than smaller non-spherical seeds, their number decreasing monotonically with depth, while the number of non-spherical seeds increased from the surface to 10.0–12.5 cm, decreasing thereafter. Larger seeds, spherical or non-spherical, had essentially the same depth–number distribution. In very small seeds (≤0.014 mm3; approximately 75% of the 29,740 total seeds), almost all spherical and non-spherical seeds were at depths at which non-photosynthetic viable growth would be unsustainable. This fraction reduced as the size of seeds increased, but it never fell below 50% and was only rarely less than 80%. The implications of these high values for aboveground recruitment are discussed in terms of the density of seeds.
Exploring the Utility of Dutch Question Answering Datasets for Human Resource Contact Centres
Authors: van Toledo Chaïm , Schraagen Marijn , van Dijk Friso , Brinkhuis Matthieu , Spruit Marco
Published: October 2022

We explore the use case of question answering (QA) by a contact centre for 130,000 Dutch government employees in the domain of questions about human resources (HR). HR questions can be answered using personnel files or general documentation, with the latter being the focus of the current research. We created a Dutch HR QA dataset with over 300 questions in the format of the Squad 2.0 dataset, which distinguishes between answerable and unanswerable questions. We applied various BERT-based models, either directly or after finetuning on the new dataset. The F1-scores reached 0.47 for unanswerable questions and 1.0 for answerable questions depending on the topic; however, large variations in scores were observed. We conclude more data are needed to further improve the performance of this task.
A Holistic Architecture for a Sales Enablement Sensing-as-a-Service Model in the IoT Environment
Authors: Olanrewaju Rashidah Funke , Khan Burhan Ul Islam , Goh Khang Wen , Hashim Aisha Hassan Abdalla , Sidek Khairul Azami Bin , Khan Zuhani Ismail , Daniyal Hamdan
Published: October 2022

Sales enablement sensing-as-a-service (SESaaS) is an organisation’s future process management for any sales management operation. With an expanding base of dynamic customer demands and the adoption of multiple technological advancements, there is a high possibility that human-centric sales management will be transformed into a fully automated form aimed at increasing productivity and being able to cater to effectively a broader customer base. A review of the relevant literature demonstrates that machine learning is one of the most prevalent techniques in analytics for predicting sales behaviour. However, SESaaS includes many features beyond the sales component. Internet-of-Things (IoT) can additionally be used for networking and data analytics to enrich sales data. Therefore, the proposed scheme introduces a novel SESaaS model capable of balancing the sales team’s needs with those of the customers to maximise profits. The proposed model also presents a novel learning scheme in the IoT environment that aids in projecting the service quality score to the final customer, thereby positively influencing the customer to pay a service fee for a superior and desired quality of experience. Unlike any existing sales management scheme, the proposed scheme offers a novel research methodology for improving sales enablement practices, emphasising service scalability, and forecasting company profit. In contrast to any existing system for sales management, the proposed scheme provides greater accuracy, higher service quality, and faster response time in its predictive strategy for projecting the cost of the adoption of SESaaS, which is not reported in any existing studies. In an extensive testing environment, it is determined that the proposed scheme achieves accuracy and service quality of approximately 98.75% and 92.91%, respectively. In addition, the proposed SESaaS model has a significantly faster response time of 1.256 s. These quantifiable outcomes were validated after being compared with commonly adopted learning programs.
Chinese Named Entity Recognition Based on BERT and Lightweight Feature Extraction Model
Authors: Yang Ruisen , Gan Yong , Zhang Chenfang
Published: October 2022

In the early named entity recognition models, most text processing focused only on the representation of individual words and character vectors, and paid little attention to the semantic relationships between the preceding and following text in an utterance, which led to the inability to handle the problem of multiple meanings of a word during recognition. To address this problem, most models introduce the attention mechanism of Transformer model to solve the problem of multiple meanings of a word in text. However, the traditional Transformer model leads to a high computational overhead due to its fully connected structure. Therefore, this paper proposes a new model, the BERT-Star-Transformer-CNN-BiLSTM-CRF model, to solve the problem of the computational efficiency of the traditional Transformer. First, the input text is dynamically generated into a character vector using the BERT model pre-trained in large-scale preconditioning to solve the problem of multiple meanings of words, and then the lightweight Star-Transformer model is used as the feature extraction module to perform local feature extraction on the word vector sequence, while the CNN-BiLSTM joint model is used to perform global feature extraction on the context in the text. The obtained feature sequences are fused. Finally, the fused feature vector sequences are input to CRF for prediction of the final results. After the experiments, it is shown that the model has a significant improvement in precision, recall and F1 value compared with the traditional model, and the computational efficiency is improved by nearly 40%.
Smart City Applications to Promote Citizen Participation in City Management and Governance: A Systematic Review
Authors: Bastos David , Fernández-Caballero Antonio , Pereira António , Rocha Nelson Pacheco
Published: October 2022

This systematic review aimed to provide a comprehensive view of (1) the purposes of research studies using smart city infrastructures to promote citizen participation in the cities’ management and governance, (2) the characteristics of the proposed solutions in terms of data sources, data quality, and data security and privacy mechanisms, as well, as strategies to incentivize citizen participation, and (3) the development stages of the applications being reported. An electronic search was conducted combining relevant databases and keywords, and 76 studies were included after a selection process. The results show a current interest in developing applications to promote citizen participation to identify urban problems and contribute to decision-making processes. Most of the included studies considered citizens as agents able to report issues (e.g., issues related to the maintenance of urban infrastructures or the mobility in urban spaces), monitor certain environmental parameters (e.g., air or acoustic pollution), and share opinions (e.g., opinions about the performance of local authorities) to support city management. Moreover, a minority of the included studies developed collaborative applications to involve citizens in decision-making processes in urban planning, the selection of development projects, and deepening democratic values. It is possible to conclude about the existence of significant research related to the topic of this systematic review, but also about the need to deepen mechanisms to guarantee data quality and data security and privacy, to develop strategies to incentivize citizen participation, and to implement robust experimental set-ups to evaluate the impact of the developed applications in daily contexts.
Evaluation of the Efficiency of Regional Airports Using Data Envelopment Analysis
Authors: Montoya-Quintero Diana María , Larrea-Serna Olga Lucía , Jiménez-Builes Jovani Alberto
Published: October 2022

Background: Small regional airports provide the necessary assistance to enable mobility in isolated areas where access is critical and costly due to poor road infrastructure and geographic constraints. Colombia’s air transportation industry has grown astonishingly quickly and dynamically over the past fifteen years. This period was coincident with the establishment and continued implementation of a public policy intended exclusively for the aviation industry and airports. However, there are currently no methods available to measure the efficiency of airports in Colombia, especially small regional airports. Methods: The research presented in this article aims to evaluate the technical efficiency of small regional airports in Colombia, using data envelopment analysis. This efficiency is achieved by considering the minimum infrastructure required to provide services and the administrative forms or properties that provide appropriate levels of this. Results: The study’s input and output data are identified, a non-parametric data envelopment analysis methodology is used, and the findings are assessed. Conclusions: The factors directly identified in the research affect the airport administration and, in the options, are available to help citizens transport optimally.
A Graphene Oxide-Angiogenin Theranostic Nanoplatform for the Therapeutic Targeting of Angiogenic Processes: The Effect of Copper-Supplemented Medium
Authors: Riela Lorenzo , Cucci Lorena Maria , Hansson Örjan , Marzo Tiziano , La Mendola Diego , Satriano Cristina
Published: October 2022

Graphene oxide (GO) nanosheets with different content in the defective carbon species bound to oxygen sp3 were functionalized with the angiogenin (ANG) protein, to create a novel nanomedicine for modulating angiogenic processes in cancer therapies. The GO@ANG nanocomposite was scrutinized utilizing UV-visible and fluorescence spectroscopies. GO exhibits pro- or antiangiogenic effects, mostly attributed to the disturbance of ROS concentration, depending both on the total concentration (i.e., >100 ng/mL) as well as on the number of carbon species oxidized, that is, the C/O ratio. ANG is considered one of the most effective angiogenic factors that plays a vital role in the angiogenic process, often in a synergic role with copper ions. Based on these starting hypotheses, the GO@ANG nanotoxicity was assessed with the MTT colorimetric assay, both in the absence and in the presence of copper ions, by in vitro cellular experiments on human prostatic cancer cells (PC-3 line). Laser confocal microscopy (LSM) cell imaging evidenced an enhanced internationalization of GO@ANG than bare GO nanosheets, as well as significant changes in cell cytoskeleton organization and mitochondrial staining compared to the cell treatments with free ANG.
Syntheses and Applications of Symmetrical Dinuclear Half-Sandwich Ruthenium(II)–Dipicolinamide Complexes as Catalysts in the Transfer Hydrogenation of Ketones
Authors: Kumah Robert Tettey , Mvelase Sabathile Thandeka , Ojwach Stephen Otieno
Published: October 2022

The treatment of [Ru(η6-p-cymene)Cl2]2 with N,N’-(1,2-phenylene)dipicolinamide (H2L1) afforded the double salt complex [{Ru(η6-p-cymene)2-µ-Cl}L1][Ru(η6-p-cymene)Cl3], (Ru1) in moderate yields. Separately, the reactions of ligands (H2L1), N,N’-(4,5 dimethyl-1,2-phenylene)dipicolinamide (H2L2), and N,N’-(4-methoxy-1,2-phenylene)dipicolinamide (H2L3) with the [Ru(η6-p-cymene)Cl2]2 in the presence of KPF6 afforded the respective dinuclear half-sandwich Ru(II) complexes [{(Ru(η6-p-cymene)2--µ-Cl}L1][PF6] (Ru2), [{(Ru(η6-p-cymene)2-µ-Cl}L2][PF6] (Ru3), and [{(Ru(η6-p-cymene)2-µ-Cl}L3][PF6] (Ru4). NMR and FT-IR spectroscopies, ESI-MS spectrometry, and elemental analyses were used to establish the molecular structures of the new dinuclear ruthenium(II) complexes. Single crystal X-ray crystallography was used to confirm the piano-stool geometry of the dinuclear complexes Ru1 and Ru4, as containing N^N chelated ligand and bridging chlorido ligands in each Ru(II) atom. The complexes (Ru1-Ru4) showed good catalytic activities at low catalyst concentrations of 0.005 mol% in the transfer hydrogenation of a wide range of ketone substrates.
Glutathione S-Transferase May Contribute to the Detoxification of (S)-(−)-Palasonin in Plutella xylostella (L.) via Direct Metabolism
Authors: Fan Qiqi , Liu Jiyuan , Li Yifan , Zhang Yalin
Published: October 2022

The control of P. xylostella primarily involves chemical insecticides, but overuse has brought about many negative effects. Our previous study reported that (S)-(−)-palasonin (PLN) is a plant-derived active substance with significant insecticidal activity against P. xylostella. However, we noticed a possible cross-resistance between (S)-(−)-palasonin and other insecticides which may be related to metabolic detoxification. In order to further explore the detoxification effect of detoxification enzymes on (S)-(−)-palasonin in P. xylostella, the effects of (S)-(−)-palasonin on enzyme activity and transcription level were determined, and the detoxification and metabolism of GSTs on (S)-(−)-palasonin were studied by in vitro inhibition and metabolism experiments. During this study, GST enzyme activity was significantly increased in P. xylostella after (S)-(−)-palasonin treatment. The expression levels of 19 GSTs genes were significantly increased whereas the expression levels of 1 gene decreased. Furthermore, (S)-(−)-palasonin is shown to be stabilized with GSTs and metabolized GSTs (GSTd1, GSTd2, GSTs1 and GSTs2) in vitro, with the highest metabolic rate of 80.59% for GSTs1. This study advances the beneficial utilization of (S)-(−)-palasonin as a botanical pesticide to control P. xylostella in the field.
Laboratory Evaluation of Pupal Parasitoids for Control of the Cornsilk Fly Species, Chaetopsis massyla and Euxesta eluta
Authors: Allan Sandra A. , Geden Christopher J. , Sobel J. Lanette
Published: October 2022

Cornsilk flies are serious pests of sweet corn through damage to cobs and secondary fungal establishment. As pupae are generally outside the infested cob on the ground, there can be potential for use of pupal parasitoids for control. Two species of gregarious parasitoids, Muscidifurax raptorellus and Nasonia vitripennis, and three species of solitary parasitoids, Spalangia endius, Spalangia cameroni and Muscidifurax raptor, were evaluated against pupae of the two cornsilk fly species, Euxesta eluta and Chaetopsis massyla. House fly pupae, the most common host for most of the parasitoids, were included for comparison. All of the parasitoids killed and successfully parasitized pupae of the two cornsilk fly species at rates that were similar to house fly pupae. Adult parasitoids that emerged from cornsilk fly hosts were somewhat smaller than parasitoids reared from house flies and had proportionally fewer females. These parasitoids, which are widely and commercially available for filth fly control, warrant further consideration for their potential against cornsilk flies in the field.
High Temperature Limits of Survival and Oviposition of Phormia regina (Meigen) and Lucilia sericata (Meigen)
Authors: Monzon Michael A. , Weidner Lauren M. , Rusch Travis W. , Nehrozoglu Selen , Hamilton George
Published: October 2022

The temperature dependent development rates of blow flies allow blow flies to be used as biological clocks in forensic death investigations. However, the upper thermal limits of adult survival and oviposition, both required for producing larvae, remains largely unknown. Therefore, in this study we examined the impact of a range of temperatures between 37 °C and 44 °C on the likelihood of survival and egg-laying behavior of two species of medicolegal forensic importance, Lucilia sericata (Meigen) and Phormia regina (Meigen) (Diptera: Calliphoridae). To quantify the upper temperature limits of survival, adult fly colonies were exposed to 37 °C, 41 °C, 42 °C, 43 °C, and 44 °C for 24 h. Similarly for oviposition trials, adults of both species were exposed to 40 °C, 42 °C, and 43 °C with P. regina oviposition also observed at 41 °C. Trials lasted for 24 h with oviposition substrate replenished at the 12 h mark. A yes/no determination on egg deposition was made, eggs were counted, and a yes/no determination was made on egg hatch. Survival did not differ by species (p = 0.096). Overall, survival decreased with increasing temperatures, with ~100% at 37 °C, ~50% at 41 °C, ~37% at 42 °C, ~15% at 43 °C and 0% at 44 °C. Lucilia sericata laid eggs capable of hatch up to 43 °C, while Phormia regina egg-hatch was observed up to 41 °C. These results indicate a greater thermal tolerance of adult survival than for egg deposition and successful egg hatch, which supports previous experiments indicating blow flies stop laying eggs at sub-lethal temperatures. Furthermore, these data indicate that adult blow flies may find remains at or near time of death but may delay egg deposition until temperatures drop below an acceptable threshold.
Effects of Color and Light Intensity on the Foraging and Oviposition Behavior of Culex pipiens biotype molestus Mosquitoes
Authors: Hellhammer Fanny , Heidtmann Hella , Freise Fritjof , Becker Stefanie C.
Published: October 2022

Mosquitoes are the most important vector of arboviruses; thus, controlling mosquito population is a key point for controlling these diseases. Two major factors that influence mosquito population size are the availability of blood hosts and suitable oviposition sites. Behavioral mechanisms by which Culex pipiens biotype molestus mosquitoes locate their hosts or oviposition sites are influenced by physical and chemical factors. The present study evaluated the impact of the colors (for human eyes) red, green, blue and yellow in combination with different light intensities on preferences for oviposition and foraging sites under laboratory conditions. We identified the color red as the overall favored color for both target behaviors, which was only surpassed by black as the foraging stimulus. Altogether, we described two new inexpensive and simple bioassays, which can be used as a mosquito-tracking method for behavioral tests and as an oviposition trap to monitor Culex pipiens biotype molestus populations.
Protein Content and Amino Acid Profiles of Selected Edible Insect Species from the Democratic Republic of Congo Relevant for Transboundary Trade across Africa
Authors: Nsevolo Miankeba Papy , Taofic Alabi , Kiatoko Nkoba , Mutiaka Kambashi , Francis Frédéric , Caparros Megido Rudy
Published: October 2022

This study analyzed the protein content of ten edible insect species (using the Dumas method), then focused on the amino acid (AA) profiles of the six major commercially relevant species using HPLC (high-pressure (or performance) liquid chromatography). The protein contents varied significantly from 46.1% to 52.9% (dry matter); the Orthoptera representative yielding both the highest protein content and the highest values in three essential amino acids (EAAs). Regarding Lepidoptera species, the protein content of Saturniidae varied more than for Notodontidae. Imbrasia ertli gave the best example of a species that could be suggested for dietary supplementation of cereal-based diets, as the sample contained the highest values in five EAAs and for the EAA index. Furthermore, first-limiting AAs in the selected insects have also been pointed out (based on a species-specific AA score), supporting that the real benefit from eating insects is correlated to a varied diet. Additionally, preliminary insights into AA distribution patterns according to taxa provided three clusters based on protein quality and should be completed further to help tailor prescriptions of dietary diets. Since the AA composition of the selected insects was close to the FAO/WHO EAA requirement pattern for preschool children and met the requirements of 40% EAAs with high ratio EAAs/NEAAs, the current study endorses reports of edible insects as nutrient-rich and sustainable protein sources.
Characteristics of Primary Bradyarrhythmia in Hypertrophic Cardiomyopathy: A 10-Year, Single-Center Analysis
Authors: Zhang Hong-Da , Tang Min , Zhang Jing-Tao
Published: October 2022

Background: Secondary bradyarrhythmias in hypertrophic cardiomyopathy (HCM) have been extensively reported. The prevalence and characteristics of primary bradyarrhythmias in HCM have yet to be investigated. Methods: We retrospectively enrolled 101 consecutive patients with HCM who were referred to the arrhythmia center from May 2010 to October 2020. The clinical features of patients with bradyarrhythmias were analyzed. Results: Twenty-nine (28.7%) patients had primary bradyarrhythmias, and six (5.9%) patients had secondary third-degree atrioventricular block (AVB). Of the 29 patients, 17 (58.6%) had sinus node dysfunction (SND), 14 (48.3%) had AVB, and two (6.9%) had both SND and AVB. The median age was 62 years old, and 69% were male. Six (20.7%) patients had left ventricular obstructive outflow tract obstruction, 15 (51.7%) had a history of syncope, and one (3.4%) had a family history of HCM. Most patients (86.2%) had New York Heart Association functional class I or II, and the median left ventricular ejection fraction was 63%. A total of 22 patients received pacemaker implantation, including 17 (77.3%) dual-chamber pacing, four (18.2%) single-chamber ventricular pacing, and one (4.5%) cardiac resynchronization therapy. Conclusions: Primary bradyarrhythmias need to be evaluated in HCM patients with arrhythmia-related symptoms. Patients with HCM might need pacemaker implantation for primary bradyarrhythmias.