bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeting DNA Homologous Repair Proficiency With Concomitant Topoisomerase II and c-Abl Inhibition
Authors: Siddiqui Arafat , Tumiati Manuela , Joko Alia , Sandholm Jouko , Roering Pia , Aakko Sofia , Vainionpää Reetta , Kaipio Katja , Huhtinen Kaisa , Kauppi Liisa , Tuomela Johanna , Hietanen Sakari
Published: September 2021

Critical DNA repair pathways become deranged during cancer development. This vulnerability may be exploited with DNA-targeting chemotherapy. Topoisomerase II inhibitors induce double-strand breaks which, if not repaired, are detrimental to the cell. This repair process requires high-fidelity functional homologous recombination (HR) or error-prone non-homologous end joining (NHEJ). If either of these pathways is defective, a compensatory pathway may rescue the cells and induce treatment resistance. Consistently, HR proficiency, either inherent or acquired during the course of the disease, enables tumor cells competent to repair the DNA damage, which is a major problem for chemotherapy in general. In this context, c-Abl is a protein tyrosine kinase that is involved in DNA damage-induced stress. We used a low-dose topoisomerase II inhibitor mitoxantrone to induce DNA damage which caused a transient cell cycle delay but allowed eventual passage through this checkpoint in most cells. We show that the percentage of HR and NHEJ efficient HeLa cells decreased more than 50% by combining c-Abl inhibitor imatinib with mitoxantrone. This inhibition of DNA repair caused more than 87% of cells in G2/M arrest and a significant increase in apoptosis. To validate the effect of the combination treatment, we tested it on commercial and patient-derived cell lines in high-grade serous ovarian cancer (HGSOC), where chemotherapy resistance correlates with HR proficiency and is a major clinical problem. Results obtained with HR-proficient and deficient HGSOC cell lines show a 50–85% increase of sensitivity by the combination treatment. Our data raise the possibility of successful targeting of treatment-resistant HR-proficient cancers.
Somatic Mutations in Oncogenes Are in Chronic Myeloid Leukemia Acquired De Novo via Deregulated Base-Excision Repair and Alternative Non-Homologous End Joining
Authors: Curik Nikola , Polivkova Vaclava , Burda Pavel , Koblihova Jitka , Laznicka Adam , Kalina Tomas , Kanderova Veronika , Brezinova Jana , Ransdorfova Sarka , Karasova Dominika , Rejlova Katerina , Bakardjieva Marina , Kuzilkova Daniela , Kundrat David , Linhartova Jana , Klamova Hana , Salek Cyril , Klener Pavel , Hrusak Ondrej , Machova Polakova Katerina
Published: September 2021

Somatic mutations are a common molecular mechanism through which chronic myeloid leukemia (CML) cells acquire resistance to tyrosine kinase inhibitors (TKIs) therapy. While most of the mutations in the kinase domain of BCR-ABL1 can be successfully managed, the recurrent somatic mutations in other genes may be therapeutically challenging. Despite the major clinical relevance of mutation-associated resistance in CML, the mechanisms underlying mutation acquisition in TKI-treated leukemic cells are not well understood. This work demonstrated de novo acquisition of mutations on isolated single-cell sorted CML clones growing in the presence of imatinib. The acquisition of mutations was associated with the significantly increased expression of the LIG1 and PARP1 genes involved in the error-prone alternative nonhomologous end-joining pathway, leading to genomic instability, and increased expression of the UNG, FEN and POLD3 genes involved in the base-excision repair (long patch) pathway, allowing point mutagenesis. This work showed in vitro and in vivo that de novo acquisition of resistance-associated mutations in oncogenes is the prevalent method of somatic mutation development in CML under TKIs treatment.
Robotic Versus Laparoscopic Distal Pancreatectomy for Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-Analysis
Authors: Feng Qingbo , Jiang Chuang , Feng Xuping , Du Yan , Liao Wenwei , Jin Hongyu , Liao Mingheng , Zeng Yong , Huang Jiwei
Published: September 2021

Background

Robotic distal pancreatectomy (RDP) and laparoscopic distal pancreatectomy (LDP) are the two principal minimally invasive surgical approaches for patients with pancreatic body and tail adenocarcinoma. The use of RDP and LDP for pancreatic ductal adenocarcinoma (PDAC) remains controversial, and which one can provide a better R0 rate is not clear.

Methods

A comprehensive search for studies that compared roboticversuslaparoscopic distal pancreatectomy for PDAC published until July 31, 2021, was conducted. Data on perioperative outcomes and oncologic outcomes (R0-resection and lymph node dissection) were subjected to meta-analysis. PubMed, Cochrane Central Register, Web of Science, and EMBASE were searched based on a defined search strategy to identify eligible studies before July 2021.

Results

Six retrospective studies comprising 572 patients (152 and 420 patients underwent RDP and LDP) were included. The present meta-analysis showed that there were no significant differences in operative time, tumor size, and lymph node dissection between RDP and LDP group. Nevertheless, compared with the LDP group, RDP results seem to demonstrate a possibility in higher R0 resection rate (p<0.0001).

Conclusions

This systematic review and meta-analysis suggest that RDP is a technically and oncologically safe and feasible approach for selected PDAC patients. Large randomized and controlled prospective studies are needed to confirm this data.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier [CRD42021269353].
Dynamic Interactions of Transcription Factors and Enhancer Reprogramming in Cancer Progression
Authors: Zboril Emily , Yoo Hannah , Chen Lizhen , Liu Zhijie
Published: September 2021

While improved tumor treatment has significantly reduced the overall mortality rates, invasive progression including recurrence, therapy resistance and metastasis contributes to the majority of deaths caused by cancer. Enhancers are essential distal DNA regulatory elements that control temporal- or spatial-specific gene expression patterns during development and other biological processes. Genome-wide sequencing has revealed frequent alterations of enhancers in cancers and reprogramming of distal enhancers has emerged as one of the important features for tumors. In this review, we will discuss tumor progression-associated enhancer dynamics, its transcription factor (TF) drivers and how enhancer reprogramming modulates gene expression during cancer invasive progression. Additionally, we will explore recent advancements in contemporary technology including single-cell sequencing, spatial transcriptomics and CUT&RUN, which have permitted integrated studies of enhancer reprogrammingin vivo. Given the essential roles of enhancer dynamics and its drivers in controlling cancer progression and treatment outcome, understanding these changes will be paramount in mitigating invasive events and discovering novel therapeutic targets.
Nanostructured Lipid Carrier–Mediated Transdermal Delivery of Aceclofenac Hydrogel Present an Effective Therapeutic Approach for Inflammatory Diseases
Authors: Garg Neeraj K. , Tandel Nikunj , Bhadada Sanjay Kumar , Tyagi Rajeev K.
Published: September 2021

Aceclofenac (ACE), a cyclooxygenase-2 inhibitor, is the derivative of the diclofenac group that has been in use for the symptomatic treatment of systemic inflammatory autoimmune disease, rheumatoid arthritis (RA). Partial solubility, high lipophilic nature, and stability challenge its use in developing topical formulations. Hence, we developed and characterized nanostructured lipid carrier (NLC)–based ACE (ACE-NLC) hydrogel for an efficient transdermal delivery. NLC microemulsion was prepared using different lipids by various methods and was characterized with respect to particle size, zeta potential, surface morphology, and drug encapsulation efficiency. The optimized NLC formulation was incorporated into Carbopol® 940 gel, and this arrangement was characterized and compared with the existing marketed gel (Mkt-gel) formulation to assess in vitro drug release, rheology, texture profile, in vivo skin retention and permeation, and stability. Furthermore, prepared and characterized ACE-loaded NLC formulation was evaluated for skin integrity and fitted in a dermatokinetic model. The results of this study confirmed the spherical shape; smooth morphology and nanometric size attested by Zetasizer and scanning and transmission electron microcopy; and stability of the ACE-NLC formulation. The ACE-NLC-gel formulation showed good rheological and texture characteristics, and better skin distribution in the epidermis and dermis. Moreover, ACE-NLC permeated deeper in the skin layers and kept the skin integrity intact. Overall, NLC-based gel formulation of ACE might be a promising nanoscale lipid carrier for topical application when compared with the conventional Mkt-gel formulation.
A Comprehensive Review of Genus Sanguisorba: Traditional Uses, Chemical Constituents and Medical Applications
Authors: Zhou Ping , Li Jingyan , Chen Qi , Wang Long , Yang Jing , Wu Anguo , Jiang Nan , Liu Yuanzhi , Chen Jianping , Zou Wenjun , Zeng Jing , Wu Jianming
Published: September 2021

Genus Sanguisorba (family: Rosaceae) comprises nearly 148 species, distributed widely across the temperate and subtropical regions of the Northern Hemisphere. Sanguisorba officinalis L. (S. officinalis) has been used as a hemostatic and scald treating medicine in China for a long time. Numerous studies have demonstrated that plant extracts or monomers from S. officinalis exhibit several pharmacological effects, such as anti-cancer, anti-virus, anti-inflammation, anti-bacteria, neuroprotective and hepatoprotective effects. The other species of genus Sanguisorba are also being studied by researchers worldwide. Sanguisorba minor Scop. (S. minor), as an edible wild plant, is a common ingredient of the Mediterranean diet, and its young shoots and leaves are often mixed with traditional vegetables and consumed as salad. Reports on genus Sanguisorba available in the current literature were collected from Google Scholar, Web of Science, Springer, and PubMed. The Plant List (http://www.theplantlist.org./tpl1.1/search?q=Sanguisorba), International Plant Name Index (https://www.ipni.org/?q=Sanguisorba) and Kew Botanical Garden (http://powo.science.kew.org/) were used for obtaining the scientific names and information on the subspecies and cultivars. In recent years, several in vivo and in vitro experiments have been conducted to reveal the active components and effective monomers of S. officinalis and S. minor. To date, more than 270 compounds have been isolated and identified so far from the species belonging to genus Sanguisorba. Numerous reports on the chemical constituents, pharmacologic effects, and toxicity of genus Sanguisorba are available in the literature. This review provides a comprehensive understanding of the current traditional applications of plants, which are supported by a large number of scientific experiments. Owing to these promising properties, this species is used in the treatment of various diseases, including influenza virus infection, inflammation, Alzheimer’s disease, type 2 diabetes and leukopenia caused by bone marrow suppression. Moreover, the rich contents and biological effects of S. officinalis and S. minor facilitate these applications in dietary supplements and cosmetics. Therefore, the purpose of this review is to summarize the recent advances in the traditional uses, chemical constituents, pharmacological effects and clinical applications of genus Sanguisorba. The present comprehensive review may provide new insights for the future research on genus Sanguisorba.
Lactobacillus casei Strain Shirota Enhances the Ability of Geniposide to Activate SIRT1 and Decrease Inflammation and Oxidative Stress in Septic Mice
Authors: Mai Chao , Qiu Li , Zeng Yong , Tan Xingqin
Published: September 2021

Gardenia jasminoides Ellis is rich in geniposide, which can be transformed into the anti-oxidant and anti-inflammatory agent genipin. Genipin exhibits greater efficacy than geniposide, but it is unstable and difficult to preserve. In this study, a mouse model for sepsis was established by cecal ligation and puncture, and then we explored the effects and mechanism of Lactobacillus casei strain Shirota (LcS) on the enhancement of the ability of geniposide to reduce sepsis and decrease inflammatory and oxidative levels in mice by the regulation of sirtuin type 1 (SIRT1). The mice were evaluated and analyzed by the open field test, Morris water maze test, flow cytometry, kit assay, qPCR, and western blot. The LcS + geniposide increased the survival rate in mice with sepsis, and increased the total travel distance, number of times the mice stood up, amount of time the mice spent grooming their fur, duration in the target quadrant, and crossing area number. The testing of mouse nerve cells showed that LcS + geniposide reduced the rate of nerve cell apoptosis caused by sepsis. LcS + geniposide also decreased the amount of inflammatory-related indicators of TNF-α, IL-6, and IL-1β, and the oxidation-related levels of malondialdehyde (MDA) in the hippocampi of septic mice, and it increased the oxidase activities of superoxide dismutase (SOD) and catalase (CAT). Additionally, LcS + geniposide increased the SOD1, SOD2, and CAT mRNA expression in the hippocampi of mice with sepsis and decreased the expression of TNF-α, IL-1β, NF-κB, and p53 mRNA. LcS+geniposide also increased the SIRT1 protein expression and decreased the Ac-FOXO1, Ac-NF-κB, and Ac-p53 protein expression in the hippocampi of mice with sepsis. We also observed that LcS + geniposide decreased the inflammatory and oxidative damage in the mice with sepsis. The effect of LcS + geniposide was similar to that of the drug dexamethasone and stronger than the effect of geniposide utilized alone. LcS also enhanced the ability of geniposide to activate SIRT1 and decrease the inflammation and oxidative stress in the septic mice, and it achieved an effect same with that obtained by the use of the drug dexamethasone.
The Transient Receptor Potential Vanilloid 4 Channel and Cardiovascular Disease Risk Factors
Authors: Goto Kenichi , Kitazono Takanari
Published: September 2021

Vascular endothelial cells regulate arterial tone through the release of nitric oxide and other diffusible factors such as prostacyclin and endothelium derived hyperpolarizing factors. Alongside these diffusible factors, contact-mediated electrical propagation from endothelial cells to smooth muscle cells via myoendothelial gap junctions, termed endothelium-dependent hyperpolarization (EDH), plays a critical role in endothelium-dependent vasodilation in certain vascular beds. A rise in intracellular Ca2+ concentration in endothelial cells is a prerequisite for both the production of diffusible factors and the generation of EDH, and Ca2+ influx through the endothelial transient receptor potential vanilloid 4 (TRPV4) ion channel, a nonselective cation channel of the TRP family, plays a critical role in this process in various vascular beds. Emerging evidence suggests that the dysregulation of endothelial TRPV4 channels underpins endothelial dysfunction associated with cardiovascular disease (CVD) risk factors, including hypertension, obesity, diabetes, and aging. Because endothelial dysfunction is a precursor to CVD, a better understanding of the mechanisms underlying impaired TRPV4 channels could lead to novel therapeutic strategies for CVD prevention. In this mini review, we present the current knowledge of the pathophysiological changes in endothelial TRPV4 channels associated with CVD risk factors, and then explore the underlying mechanisms involved.
Comparative Transcriptome Analysis Revealed Candidate Genes Potentially Related to Desiccation Sensitivity of Recalcitrant Quercus variabilis Seeds
Authors: Li Dongxing , Li Yingchao , Qian Jialian , Liu Xiaojuan , Xu Huihui , Zhang Guowei , Ren Junjie , Wang Libing , Zhang Lu , Yu Haiyan
Published: September 2021

Chinese cork oak (Quercus variabilis) is a widely distributed and highly valuable deciduous broadleaf tree from both ecological and economic perspectives. Seeds of this species are recalcitrant, i.e., sensitive to desiccation, which affects their storage and long-term preservation of germplasm. However, little is known about the underlying molecular mechanism of desiccation sensitivity of Q. variabilis seeds. In this study, the seeds were desiccated with silica gel for certain days as different treatments from 0 (Control) to 15 days (T15) with a gradient of 1 day. According to the seed germination percentage, four key stages (Control, T2, T4, and T11) were found. Then the transcriptomic profiles of these four stages were compared. A total of 4,405, 4,441, and 5,907 differentially expressed genes (DEGs) were identified in T2 vs. Control, T4 vs. Control, and T11 vs. Control, respectively. Among them, 2,219 DEGs were overlapped in the three comparison groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that these DEGs were enriched into 124 pathways, such as “Plant hormone signal transduction” and “Glycerophospholipid metabolism”. DEGs related to hormone biosynthesis and signal transduction (ZEP, YUC, PYR, ABI5, ERF1B, etc.), stress response proteins (LEA D-29, HSP70, etc.), and phospholipase D (PLD1) were detected during desiccation. These genes and their interactions may determine the desiccation sensitivity of seeds. In addition, group specific DEGs were also identified in T2 vs. Control (PP2C62, UNE12, etc.), T4 vs. Control (WRKY1-like, WAK10, etc.), and T11 vs. Control (IBH1, bZIP44, etc.), respectively. Finally, a possible work model was proposed to show the molecular regulation mechanism of desiccation sensitivity in Q. variabilis seeds. This is the first report on the molecular regulation mechanism of desiccation sensitivity of Q. variabilis seeds using RNA-Seq. The findings could make a great contribution to seed storage and long-term conservation of recalcitrant seeds in the future.
Cannabis Glandular Trichomes: A Cellular Metabolite Factory
Authors: Tanney Cailun A. S. , Backer Rachel , Geitmann Anja , Smith Donald L.
Published: September 2021

Cannabis has been legalized for recreational use in several countries and medical use is authorized in an expanding list of countries; markets are growing internationally, causing an increase in demand for high quality products with well-defined properties. The key compounds of Cannabis plants are cannabinoids, which are produced by stalked glandular trichomes located on female flowers. These trichomes produce resin that contains cannabinoids, such as tetrahydrocannabinolic acid and cannabidiolic acid, and an array of other secondary metabolites of varying degrees of commercial interest. While growers tend to focus on improving whole flower yields, our understanding of the “goldmines” of the plant – the trichomes – is limited despite their being the true source of revenue for a multi-billion-dollar industry. This review aims to provide an overview of our current understanding of cannabis glandular trichomes and their metabolite products in order to identify current gaps in knowledge and to outline future research directions.
Differential Effects of Exogenous Glomalin-Related Soil Proteins on Plant Growth of Trifoliate Orange Through Regulating Auxin Changes
Authors: Liu Rui-Cheng , Gao Wei-Qin , Srivastava Anoop Kumar , Zou Ying-Ning , Kuča Kamil , Hashem Abeer , Abd_Allah Elsayed Fathi , Wu Qiang-Sheng
Published: September 2021

Multiple functions of glomalin released by arbuscular mycorrhizal fungi are well-recognized, whereas the role of exogenous glomalins including easily extractable glomalin-related soil protein (EE-GRSP) and difficultly extractable glomalin-related soil protein (DE-GRSP) is unexplored for plant responses. Our study was carried out to assess the effects of exogenous EE-GRSP and DE-GRSP at varying strengths on plant growth and chlorophyll concentration of trifoliate orange (Poncirus trifoliata) seedlings, along with changes in root nutrient acquisition, auxin content, auxin-related enzyme and transporter protein gene expression, and element contents of purified GRSP. Sixteen weeks later, exogenous GRSP displayed differential effects on plant growth (height, stem diameter, leaf number, and biomass production): the increase by EE-GRSP and the decrease by DE-GRSP. The best positive effect on plant growth occurred at exogenous EE-GRSP at ½ strength. Similarly, the GRSP application also differently affected total chlorophyll content, root morphology (total length, surface area, and volume), and root N, P, and K content: positive effect by EE-GRSP and negative effect by DE-GRSP. Exogenous EE-GRSP accumulated more indoleacetic acid (IAA) in roots, which was associated with the upregulated expression of root auxin synthetic enzyme genes (PtTAA1, PtYUC3, and PtYUC4) and auxin influx transporter protein genes (PtLAX1, PtLAX2, and PtLAX3). On the other hand, exogenous DE-GRSP inhibited root IAA and indolebutyric acid (IBA) content, associated with the downregulated expression of root PtTAA1, PtLAX1, and PtLAX3. Root IAA positively correlated with root PtTAA1, PtYUC3, PtYUC4, PtLAX1, and PtLAX3 expression. Purified EE-GRSP and DE-GRSP showed similar element composition but varied in part element (C, O, P, Ca, Cu, Mn, Zn, Fe, and Mo) concentration. It concluded that exogenous GRSP triggered differential effects on growth response, and the effect was associated with the element content of pure GRSP and the change in auxins and root morphology. EE-GRSP displays a promise as a plant growth biostimulant in citriculture.
Skill Level in Tennis Serve Return Is Related to Adaptability in Visual Search Behavior
Authors: Rosker Jernej , Majcen Rosker Ziva
Published: September 2021

Analyzing visual search strategies in tennis is primarily focused on studying relationships between visual behavior and tennis performance. However, diverse movement characteristics among different servers suggest the importance of adjusting the visual search strategies of an individual while playing against different opponents. The aim of this study was to analyze whether visual search strategies can be attributed to the individual server and the returning player during the tennis serve return or return performance. Seventeen tennis players were enrolled in this study (five international players and 12 national players) producing a sample of 1,020 returns measured with mobile eye trackers. The random forest machine learning model was used to analyze the ability to classify the returning player [area under the curve (AUC): 0.953], individual server (AUC: 0.686), and return performance category (AUC: 0.667) based on the location and duration of the focal vision fixation. In international tennis players, the higher predictability of the server was observed as compared with national level players (AUC: 0.901 and 0.834, respectively). More experienced tennis players presented with a higher ability to adjust their visual search strategies to different servers. International players also demonstrated anticipatory visual behavior during the tossing hand movement and superior information pickup during the final phases of the stroke of a server.
Agency: What Does It Mean to Be a Human Being?
Authors: Williams Richard N. , Gantt Edwin E. , Fischer Lane
Published: September 2021

This paper will look at the results of what has been termed “the crisis of modernism” and the related rise of postmodern perspectives in the 19th and 20th centuries. It concentrates on what is arguably the chief casualty of this crisis – human agency – and the social science that has developed out of the crisis. We argue that modern and postmodern social science ultimately obviate human agency in the understanding of what it means to be a human being. Attention is given to the contemporary intellectual world and the way in which it has been deeply informed by neo-Hegelian and other postmodern scholarly trends, particularly in accounting for how agency has come to play little role in social science understanding of human action. The paper also offers an alternative conception of human agency to the commonly endorsed libertarian model of free choice. Finally, the paper argues that this view of agency preserves meaning and purpose in human action and counters the pervasive social science worldview that sacrifices agency and meaning to powerful invisible abstractions.
Kinematic and Neuromuscular Measures of Intensity During Drop Jumps in Female Volleyball Players
Authors: Torres-Banduc Maximiliano , Ramirez-Campillo Rodrigo , Andrade David Cristobal , Calleja-González Julio , Nikolaidis Pantelis Theo , McMahon John J. , Comfort Paul
Published: September 2021

The aim of this study was to assess drop jump (DJ) performance variables (jump height, contact time, and reactive strength index) concomitant to surface electromyography (sEMG) of lower limb muscles during DJs from different drop heights (intensities). The eccentric and concentric phase sEMG from the gastrocnemius medialis, biceps femoris, and vastus medialis muscles were assessed during all tests, with sEMG activity normalized to maximal voluntary isometric contraction (MVIC). In a cross-sectional, study, 10 amateur female volleyball players (age 22.1 ± 1.8 years; body mass 72.9 ± 15.2 kg; height 1.70 ± 0.08 m) completed DJs from six heights [15–90 cm (DJ15 to DJ90)]. During DJs there was no jump-target box to rebound on to. Results of one-way analysis of variance (ANOVA) showed that the jump height, contact time, and reactive strength index were not significantly (p > 0.05) different between drop heights. Mean biceps femoris eccentric and concentric sEMG ranged from 27 to 50%, although without significant differences between drop heights. Mean gastrocnemius medialis eccentric and concentric sEMG remained relatively constant (∼60–80% MVIC) across DJs heights, although eccentric values reached 90–120% MVIC from DJ75 to DJ90. Mean variations of ∼50–100% MVIC for eccentric and ∼50–70% MVIC for concentric sEMG activations were observed in the vastus medialis across DJs heights. The biceps femoris eccentric/concentric sEMG ratio during DJ45 (i.e., 1.0) was lower (p = 0.03) compared to the ratio observed after DJ90 (i.e., 3.2). The gastrocnemius medialis and vastus medialis eccentric/concentric sEMG ratio were not significantly different between drop heights. In conclusion, jumping performance and most neuromuscular markers were not sensitive to DJ height (intensity) in amateur female volleyball athletes.
Products With High Purchase Frequency Require Greater Inhibitory Control: An Event-Related Potential Study
Authors: Tsuji Koki , Shibata Midori , Terasawa Yuri , Umeda Satoshi
Published: September 2021

One’s past behavior influences their present behavior. The effects of such response history have often been tested using response inhibition tasks. Since previous studies have highlighted the effect of immediate action history formed directly before the subsequent response in a laboratory environment, we aimed to elucidate the longer-term effects of response history, using repetitive and habitual consumer behavior in daily life as the response history. We used event-related potentials recorded in a Go/No-go task to investigate brain activity related to inhibitory control, hypothesizing that stimuli with a high frequency of choice in everyday life would elicit stronger inhibition-related activity, that is, the No-go-N2 component. Participants were asked to evaluate the frequency of purchase and use of some products, such as food and drink or social networking services (SNS) in everyday situations. Images of each product were assigned as stimuli in the Go and No-go trials according to the frequency of choice. The results showed that frequently purchased No-go stimuli yielded a larger amplitude of the No-go-N2 component and a negative shift between 200 and 300ms after the presentation of No-go stimuli. The results suggest that frequently chosen products evoke stronger inhibition conflicts and require greater cognitive control to withhold a response. Our findings showed that repeated purchase behavior in daily life forms a response history and has a long-term influence on the inhibition of even simple approaching behaviors, such as button pressing.
The Association Between Financial Hardship and Mental Health Difficulties Among Adult Wage Earners During the COVID-19 Pandemic in Bangladesh: Findings From a Cross-Sectional Analysis
Authors: Sultana Mst. Sadia , Khan Abid Hasan , Hossain Sahadat , Islam Tauhidul , Hasan M. Tasdik , Ahmed Helal Uddin , Li Zezhi , Khan Jahangir A. M.
Published: September 2021

Background: The ongoing COVID-19 pandemic has created several challenges including financial burdens that may result in mental health conditions. This study was undertaken to gauge mental health difficulties during the COVID-19 pandemic and gain an insight into wage earners' mental health.

Method: This cross-sectional study was conducted through an online survey. A t total of 707 individual Bangladeshi wage earners were enrolled between 20 and 30 May 2020. The questionnaire had sections on sociodemographic information, COVID-19 related questions, PHQ-9 and GAD-7 scales. STATA version 14.1 program was used to carry out all the analyses.

Results: The study revealed that 58.6 and 55.9% of the respondents had moderate to severe anxiety and depressive symptoms, respectively. The total monthly income was <30,000 BDT (353.73USD) and displayed increased odds of suffering from depressive symptoms (OR = 4.12; 95% CI: 2.68–6.34) and anxiety (OR = 3.31; 95% CI: 2.17–5.03). Participants who did not receive salary income, had no income source during the pandemic, had financial problems, and inadequate food supply and were more likely to suffer from anxiety and depressive symptoms (p ≤ 0.01). Perceiving the upcoming financial crisis as a stressor was a potential risk factor for anxiety (OR = 1.91; 95% CI:1.32–2.77) and depressive symptoms (OR = 1.50; 95% CI:1.04–2.16).

Limitations: The online survey method used in this study limits the generalizability of the findings and self-reported answers might include selection and social desirability bias as a community-based survey was not possible during the pandemic.

Conclusion: Wage earners in a low resource setting like Bangladesh require mental health attention and financial consideration to deal with mental health difficulties.
Brief Report: A Cross-Sectional Study of Anxiety Levels and Concerns of Chinese Families of Children With Special Educational Needs and Disabilities Post-first-wave of COVID-19
Authors: Su Xueyun , Cai Ru Ying , Uljarević Mirko , Van Herwegen Jo , Dukes Daniel , Yang Yufang , Peng Xiaomei , Samson Andrea C.
Published: September 2021

The COVID-19 pandemic has a multifaceted impact on mental health due to ill health, restrictions and lockdowns, and loss of employment and institutional support. COVID-19 may disproportionally impact families with special educational needs and disabilities (SEND) due to the already higher prevalence of mental health conditions in children with SEND and their parents. Therefore, it is essential to determine the short-term impact of the pandemic on the mental health of families with SEND in order to identify their ongoing health support needs. The current study aims to examine the anxiety level and concerns of children with SEND and their parents living in China. The sample consisted of 271 parents of children with SEND aged between 6 and 17 years (Mage = 8.37; SDage = 2.76). Parents completed an online survey between 10 April to 8 June 2020. Both child and parental anxiety levels and various concerns increased after the initial wave of COVID-19 when compared with retrospective pre-COVID-19 levels. Parental anxiety and concern levels were significantly higher for those living in rural areas compared to urban areas. In addition, parental and child anxiety and concern levels were significantly correlated with each other. Parental anxiety at the lowest level made a unique and significant statistical contribution to children's anxiety levels. The implications of the study findings are discussed.
Methylation Analysis in Monozygotic Twins With Treatment-Resistant Schizophrenia and Discordant Responses to Clozapine
Authors: Kikuchi Masataka , Nakazawa Takanobu , Kinoshita Makoto , Yamamori Hidenaga , Yasuda Yuka , Fujimoto Michiko , Hashimoto Ryota , Numata Shusuke
Published: September 2021

Schizophrenia is a mental illness that involves both genetic and environmental factors. Clozapine, an atypical antipsychotic, is a well-established therapy for treatment-resistant schizophrenia. In this study, we focused on a set of monozygotic twins with treatment-resistant schizophrenia in which one twin effectively responded to clozapine treatment and the other did not. Our previous study generated neurons from induced pluripotent stem (iPS) cells derived from these patients and compared the transcriptome profiles between mock- and clozapine-treated neurons. In this study, we performed genome-wide DNA methylation profiling to investigate the mechanisms underlying gene expression changes. First, we extracted the differentially methylated sites from each twin based on statistical analysis. Then, we combined the DNA methylation profiling with transcriptome profiling from our previous RNA-seq data. Among the genes with altered methylation and expression, we found the different proportions of the genes related to neuronal and synaptic functions between the clozapine responder and non-responder (35.7 and 6.7%, respectively). This trend was observed even when the basal differences between the responder and non-responder was excluded. These results suggest that effective clozapine action may correct the abnormalities of neuronal and synapse functions in schizophrenia via changes in methylation.
Comparison of the Effects of a Genetic, a Mild Encephalitis, and a Psychosocial Causal Explanation of Schizophrenia on Stigmatizing Attitudes – a Pilot Study With a Quasi-Experimental Design
Authors: Haouchet Sonja , Harder Carolin , Müller Sabine
Published: September 2021

Background: Previous research has shown that the endorsement of biogenetic causal explanations of schizophrenia is associated with stronger stigmatizing attitudes against people with schizophrenia than the endorsement of psychosocial explanations. However, little is known about whether different biogenetic causal explanation beliefs differentially affect stigmatizing attitudes. This is particularly valid for the endorsement of the mild encephalitis hypothesis of schizophrenia.

Aim: To examine to what extent different causal explanations of schizophrenia influence the desire for social distance from persons with schizophrenia.

Methods: A study with a prospective, quasi-experimental design was carried out with students in Germany (N = 333). A case vignette depicting a person with schizophrenia-typical symptoms was presented, and a social distance scale (SDS) was used to measure the stigmatizing attitude against the person described. Participants were randomly assigned to one of three groups receiving different causal explanations of schizophrenia (genetic, mild encephalitis hypothesis, or psychosocial) without treatment information.

Results: A one-way ANOVA showed that the mean SDS was lowest in the group with the mild encephalitis hypothesis explanation, followed by the genetic explanation group, and highest in the psychosocial explanation group. However, the differences between the groups were small and not significant. A subanalysis revealed a significant interaction between gender and causal explanation. Women showed a significantly lower desire for social distance than men when receiving the mild encephalitis hypothesis. Neither the study discipline nor the number of semesters of study had significant effects on the mean SDS. The differences between the mean SDS scores for the different items were much bigger than the differences for the different causal explanations. Regardless of the causal explanation, the extent of the desired social distance depends strongly on social proximity.

Conclusion: The present study fits into previous research, which has found that biogenetic beliefs were either associated with more social distance or did not yield a statistically significant association. Although we found a small gender-specific effect of the endorsement of the mild encephalitis hypothesis, we do not recommend gender-specific anti-stigmatization campaigns because they might rightly raise suspicions of dishonesty and manipulation. Rather we support recovery-oriented messages focusing on effective treatments.
Supportive or Confining? The Impact of War Metaphors From the COVID-19 Pandemic on Persons With Disabilities in Mainland China
Authors: Chen Ren-Xing , Ge Zhong-Ming , Hu Shu-Ling , Tang Wei-Zhong
Published: September 2021

Ensuring the well-being of persons with disabilities (PWDs) is a priority in the public sector during the coronavirus disease 2019 (COVID-19) pandemic. To contain this unprecedented public crisis in China, a set of nationwide anti-epidemic discourse systems centered on war metaphors has guided the epidemic's prevention and control. While the public is immersed in the joy brought by the stage victory, most ignore the situation of the disadvantaged PWDs. Accordingly, this study adopts and presents a qualitative research method to explore the impact of war metaphors on PWDs. The results showed that while there was some formal and informal support for PWDs during this period, they were increasingly marginalized. Owing to the lack of a disability lens and institutional exclusion, PWDs were placed on the margins of the epidemic prevention and control system like outsiders. Affected by pragmatism under war metaphors, PWDs are regarded as non-contributory or inefficient persons; therefore, they are not prioritized and are thus placed into a state of being voiceless and invisible. This research can provide inspiration for improving public services for PWDs in the context of COVID-19.
COVID-19 and Global Supply Chain Configuration: Economic and Emissions Impacts of Australia-China Trade Disruptions
Authors: Shi Xunpeng , Cheong Tsun Se , Zhou Michael
Published: September 2021

Economic shocks from COVID-19, coupled with ongoing US-China tensions, have raised debates around supply chain (or global value chain) organisation, with China at the centre of the storm. However, quantitative studies that consider the global and economy-wide impacts of rerouting supply chains are limited. This study examines the economic and emissions impacts of reorganising supply chains, using Australia-China trade as an example. It augments the Hypothetical Extraction Method by replacing traditional Input-Output analysis with a Computable General Equilibrium analysis. The estimation results demonstrate that in both exports and imports, a trade embargo between Australia and China – despite being compensated for by alternative supply chains—will cause gross domestic production losses and emissions increases for both countries and the world overall. Moreover, even though all other economies gain from the markets left by China, many of them incur overall gross domestic production losses and emission increases. The finding that the Association of Southeast Asian Nations and India may also suffer from an Australia-China trade embargo, despite a gain in trade volume, suggests that no country should add fuel to the fire. The results suggest that countries need to defend a rules-based trading regime and jointly address supply chain challenges.
Lagrange Point Missions: The Key to next Generation Integrated Earth Observations. DSCOVR Innovation
Authors: Valero Francisco P. J. , Marshak Alexander , Minnis Patrick
Published: September 2021

A new perspective for studying Earth processes has been soundly demonstrated by the Deep Space Climate Observatory (DSCOVR) mission. For the past 6 years, the first Earth-observing satellite orbiting at the Lagrange 1 (L1) point, the DSCOVR satellite has been viewing the planet in a fundamentally different way compared to all other satellites. It is providing unique simultaneous observations of nearly the entire sunlit face of the Earth at a relatively high temporal resolution. This capability enables detailed coverage of evolving atmospheric and surface systems over meso- and large-scale domains, both individually and as a whole, from sunrise to sunset, under continuously changing illumination and viewing conditions. DSCOVR’s view also contains polar regions that are only partially seen from geostationary satellites (GEOs). To exploit this unique perspective, DSCOVR instruments provide multispectral imagery and measurements of the Earth’s reflected and emitted radiances from 0.2 to 100 µm. Data from these sensors have been and continue to be utilized for a great variety of research involving retrievals of atmospheric composition, aerosols, clouds, ocean, and vegetation properties; estimates of surface radiation and the top-of-atmosphere radiation budget; and determining exoplanet signatures. DSCOVR’s synoptic and high temporal resolution data encompass the areas observed during the day from low Earth orbiting satellites (LEOs) and GEOs along with occasional views of the Moon. Because the LEO and GEO measurements can be easily matched with simultaneous DSCOVR data, multiangle, multispectral datasets can be developed by integrating DSCOVR, LEO, and GEO data along with surface and airborne observations, when available. Such datasets can open the door for global application of algorithms heretofore limited to specific LEO satellites and development of new scientific tools for Earth sciences. The utility of the integrated datasets relies on accurate intercalibration of the observations, a process that can be facilitated by the DSCOVR views of the Moon, which serves as a stable reference. Because of their full-disc views, observatories at one or more Lagrange points can play a key role in next-generation integrated Earth observing systems.
Az alsó bölcsességfogak juxta-apikális radiolucenciája
Authors: Janovics Kata , Soós Balázs , Lempel Edina , Bán Ágnes , Szalma József
Published: September 2021

A juxta-apikális radiolucencia (JAR) egy olyan radiológiai jel, amely jól körülhatárolt radiolucens területként jelenik meg az alsó bölcsességfogak apikális és laterális felszínén. Egyes vizsgálatok alapján a JAR jelenléte közvetlen összefüggésbe hozható a bölcsességfog eltávolítása során bekövetkező nervus alveolaris inferior sérüléssel, míg más vizsgálatokban ezt nem bizonyították. Jelen tanulmányunk célja volt a nemzetközi szakirodalom áttekintése, és a JAR fogalmának bevezetése a hazai szakirodalomba. A JAR ismerete a gyakorló fogorvos, szájsebész számára mind diagnosztikai szempontból (pl. góckutatás-góctalanítás problémaköre, fogeltávolítás indikációinak tisztázása), mind a műtéttechnika szempontjából (kevésbé ellenálló sérülékenyebb terület) jelentőséggel bír.
Description of a Eulerian–Lagrangian Approach for the Modeling of Cooling Water Droplets
Authors: Meireles Rúben , Magalhães Leandro , Silva André , Barata Jorge
Published: September 2021

The present paper describes a tool developed in-house for the modeling of free-falling water droplet cooling processes. A two-way coupling model is employed to account for the interactions between the droplets and the carrier fluid, following a Eulerian–Lagrangian approach. In addition, a stochastic separated flow technique is employed, involving random sampling of the fluctuating fluid velocity. In physical modeling, two empirical correlations are considered for determining the heat and mass transfer coefficients, with the possibility of accounting for vibrations. The numerical results indicate the preponderance of the interactions between droplet and carrier fluid at various humidity ratios.
Experimental Validation for the Performance of MR Damper Aircraft Landing Gear
Authors: Jo Bang-Hyun , Jang Dae-Sung , Hwang Jai-Hyuk , Choi Yong-Hoon
Published: September 2021

The landing gear of an aircraft serves to mitigate the vibration and impact forces transmitted from the ground to the fuselage. This paper addresses magneto-rheological (MR) damper landing gear, which provides high shock absorption efficiency and excellent stability in various landing conditions by adjusting the damping force using external magnetic field intensity. The performance and stability of an MR damper was verified through numerical simulations and drop tests that satisfied aviation regulations for aircraft landing gear. In this study, a prototype MR damper landing gear, a drop test jig, and a two-degree-of-freedom model were developed to verify the performance of the MR damper, with real-time control, for light aircraft landing gear. Two semi-active control algorithms, skyhook control and hybrid control, were applied to the MR damper landing gear. The drop tests were carried out under multiple conditions, and the results were compared with numerical simulations based on the mathematical model. It was experimentally verified that as the shock absorption efficiency increased, the landing gear’s cushioning performance significantly improved by 17.9% over the efficiency achieved with existing passive damping.
Moisture–Conductivity Calibration for Electrical Imaging of Horticultural Substrate
Authors: Zhao Peng-Fei , Fan Li-Feng , Wang Yong-Qian , Li Yang , Wang Nan , Wang Zhong-Yi , Cheng Qiang , Huang Lan
Published: September 2021

Electrical imaging studies of laboratory- and small-scale plant root zones are gaining increasing attention. However, for essential moisture–conductivity calibrations on numerous substrate columns with variability in dry density, the use of one conventional strategy is relatively laborious or complicated. Thus, in this work, a relatively convenient calibration method is presented, and the objective is to identify its feasibility and potential to assess the effects of factors (e.g., volumetric water content (VWC), and dry density) on conductivity and establish necessary moisture–conductivity curves for porous materials (e.g., soils and substrates). In the method, with a specially designed fixture, variable VWCs, dry densities and related complex conductivities of the samples can be easily acquired through static compaction. The results show that the in-phase conductivity (or magnitude of conductivity) increases with the increasing VWC or dry density, primarily owing to the increase in the dominant pore water connectivity. Moreover, the effect of dry density on conductivity is relatively smaller than that of VWC. Thus, for the substrates at dry densities with certain variability, good power law relations (R2 ≥ 0.99) between in-phase conductivity (or magnitude of conductivity) and VWC at different frequencies can be established. Overall, the proposed approach is practical, promising, and relatively time- and labor-saving.