bluuebunny/crossref_metadata_2025 huggingface dataset:
Soil Chemical Properties and Fire Severity Assessment Using VNIR Proximal Spectroscopy in Fire-Affected Abandoned Orchard of Mediterranean Croatia
Authors: Šestak Ivana , Pereira Paulo , Telak Leon Josip , Perčin Aleksandra , Hrelja Iva , Bogunović Igor
Published: January 2022

This paper aims to evaluate the ability of VNIR proximal soil spectroscopy to determine post-fire soil chemical properties and discriminate fire severity based on soil spectra. A total of 120 topsoil samples (0–3 cm) were taken from 6 ha of unburned (control (CON)) and burned areas (moderate fire severity (MS) and high fire severity (HS)) in Mediterranean Croatia within one year after the wildfire. Partial least squares regression (PLSR) and an artificial neural network (ANN) were used to build calibration models of soil pH, electrical conductivity (EC), CaCO3, plant-available phosphorus (P2O5) and potassium (K2O), soil organic carbon (SOC), exchangeable calcium (exCa), magnesium (exMg), potassium (exK), sodium (exNa), and cation exchange capacity (CEC), based on soil reflectance data. In terms of fire severity, CON samples exhibited higher average reflectance than MS and HS samples due to their lower SOC content. The PCA results pointed to the significance of the NIR part of the spectrum for extracting the variance in reflectance data and differentiation between the CON and burned area (MS and HS). DA generated 74.2% correctly classified soil spectral samples according to the fire severity. Both PLSR and ANN calibration techniques showed sensitivity to extract information from soil features based on hyperspectral reflectance, most successfully for the prediction of SOC, P2O5, exCa, exK, and CEC. This study confirms the usefulness of soil spectroscopy for fast screening and a better understanding of soil chemical properties in post-fire periods.
LEGU-MED: Developing Biodiversity-Based Agriculture with Legume Cropping Systems in the Mediterranean Basin
Authors: Martinelli Federico , Vollheyde Anna-Lena , Cebrián-Piqueras Miguel A. , von Haaren Christina , Lorenzetti Elisa , Barberi Paolo , Loreto Francesco , Piergiovanni Angela Rosa , Totev Valkov Vladimir , Bedini Alberico , Kron Morelli Roberto , Yahia Nourredine , Rezki Meriem Amina , Ouslim Sarah , Fyad-Lameche F. Z. , Bekki Abdelkader , Sikora Sanja , Rodríguez-Navarro Dulce , Camacho María , Nabbout Rania , Amil Rola , Trabelsi Darine , Yucel Derya , Yousefi Sanaz
Published: January 2022

Environmental degradation and the decrease of ecosystem service provision are currently of major concern, with current agricultural systems being a major driver. To meet our future environmental and sustainability targets a transformation of the agro-food systems and current agricultural value chain are crucial. One approach to redesign farming systems is the concept of biodiversity-based agriculture (BBA) which relies on sustainable diversification of biological components and their natural interactions in farming systems to maximize fertility, productivity, and resilience to external perturbations. Despite minimizing anthropogenic inputs, BBA is not yet able to meet all beneficial environmental objectives. BBA applied in the Mediterranean basin requires urgent innovation in approaches, methodologies, and models for small-holder traditional farming systems to ensure a stable provision of ecosystem services and better resilience to environmental stresses linked to climate change. Legumes are the backbone of the Mediterranean agro-ecosystems from ancient times, but their unique and wide biodiversity was not sufficiently valorized, especially by North-African countries. Here, we present LEGU-MED, a three-year international project funded by PRIMA initiative 2019. An international consortium was established involving five universities, 5 research institutes, and one private company from 8 countries: Italy, Germany, Spain, Algeria, Tunisia, Turkey, Lebanon, and Croatia. The main objective of this project is to put forward an international and well-integrated plan to valorize the legume agrobiodiversity of the Mediterranean in biodiversity-based farming systems and consequently enhance agro-ecosystem functions and services in the Mediterranean basin. The successful completion of LEGU-MED will have the following impacts on Mediterranean legume-based farming systems: (1) improve water use efficiency, (2) reduce the use of anthropogenic inputs through the maintenance of soil fertility, (3) enhance pollination and improve ecological connectivity with flora and fauna, (4) protect close-by wildland ecosystems, (5) enhance other ecosystem services (e.g., pest, disease, and weed suppression), and (6) provide healthier and safer protein-rich food.
Dealing with Plastic Waste from Agriculture Activity
Authors: Batista Teresa , Cansado Isabel Pestana da Paixão , Tita Barbara , Ilhéu Ana , Metrogos Luis , Mourão Paulo Alexandre Mira , Nabais João Manuel Valente , Castanheiro José Eduardo , Borges Cátia , Matos Gilda
Published: January 2022

The increase in agricultural production and food quality has forced the growing use of plastics in various activities. The plastic wastes are partially recycled in or outside Portugal; nevertheless, the contaminated wastes are sent to landfill. It is crucial to consider new models for their valorization at a regional level and from a circular economy perspective. In the scope of the Placarvões project, a study was elaborated, which included the types and quantities of plastics used in the irrigation area of the Alqueva Dam, in southern Portugal. The crops that use the most plastic are intensive olive groves, almonds, and table grapes, which represent more than 91% of total plastic waste. The production of activated carbons (ACs) is a solution to avoid plastics landfill. ACs were produced from plastic used on food packaging (PB-Samples) and sheeting film (PS-Samples) by activation with K2CO3. ACs presented well-developed textural properties (PB-K2CO3-1:1–700 and PS-K2CO3-1:1–700 exhibited a volume of 0.32 and 0.25 cm3 g−1 and an apparent surface area of 723 and 623 m2 g−1, respectively). Both ACs performed very well concerning four pesticide removals from the liquid phase. This solution is very promising, such these ACs could be applied in effluent treatments on a large scale.
Effects of Dietary Cereal and Protein Source on Fiber Digestibility, Composition, and Metabolic Activity of the Intestinal Microbiota in Weaner Piglets
Authors: Ellner Carola , Wessels Anna G. , Zentek Jürgen
Published: January 2022

This study aimed to investigate the effect of fiber-rich rye and rapeseed meal (RSM) compared to wheat and soybean meal (SBM) on fiber digestibility and the composition and metabolic activity of intestinal microbiota. At weaning, 88 piglets were allocated to four feeding groups: wheat/SBM, wheat/RSM, rye/SBM, and rye/RSM. Dietary inclusion level was 48% for rye and wheat, 25% for SBM, and 30% for RSM. Piglets were euthanized after 33 days for collection of digesta and feces. Samples were analyzed for dry matter and non-starch-polysaccharide (NSP) digestibility, bacterial metabolites, and relative abundance of microbiota. Rye-based diets had higher concentrations of soluble NSP than wheat-based diets. RSM-diets were higher in insoluble NSP compared to SBM. Rye-fed piglets showed a higher colonic and fecal digestibility of NSP (p < 0.001, p = 0.001, respectively). RSM-fed piglets showed a lower colonic and fecal digestibility of NSP than SBM-fed piglets (p < 0.001). Rye increased jejunal and colonic concentration of short-chain fatty acids (SCFA) compared to wheat (p < 0.001, p = 0.016, respectively). RSM-fed pigs showed a lower jejunal concentration of SCFA (p = 0.001) than SBM-fed pigs. Relative abundance of Firmicutes was higher (p = 0.039) and of Proteobacteria lower (p = 0.002) in rye-fed pigs compared to wheat. RSM reduced Firmicutes and increased Actinobacteria (jejunum, colon, feces: p < 0.050), jejunal Proteobacteria (p = 0.019) and colonic Bacteroidetes (p = 0.014). Despite a similar composition of the colonic microbiota, the higher amount and solubility of NSP from rye resulted in an increased fermentative activity compared to wheat. The high amount of insoluble dietary fiber in RSM-based diets reduced bacterial metabolic activity and caused a shift toward insoluble fiber degrading bacteria. Further research should focus on host–microbiota interaction to improve feeding concepts with a targeted use of dietary fiber.
Implementation of a Pig Toilet in a Nursery Pen with a Straw-Littered Lying Area
Authors: Tillmanns Michelle , Scheepens Kees , Stolte Marieke , Herbrandt Swetlana , Kemper Nicole , Fels Michaela
Published: January 2022

In this study, a pig toilet was installed on an organic pig farm, which enabled pigs to use a lying area littered with straw and keep it clean. The pig toilet was separated into a defaecation area and a urination area and nursery pigs were trained to use the urination area by means of a rewarding system. A total of 24 piglets were weaned at 6–7 weeks of age and housed in the experimental system for four-week periods. Per trial, a group of four pigs was formed, and videos were recorded on two days per week (08:00 to 18:00). Direct observation was carried out in the first and last week of each trial. In total, 1500 eliminations were video-analysed. An individual pig had an average of 7.1 ± 1.4 defaecations and 4.8 ± 0.8 urinations per day. In total, 96.4% of all urinations and 97.4% of all defaecations were performed in the pig toilet. However, most urinations took place in the defaecation area as well (90.4%). Even if the training to spatially separate defecation and urination behaviour was not successful, we showed that a pig toilet offers the possibility to create littered lying areas, possibly increasing animal welfare.
Antibiotic Resistance Patterns in Cervical Microbes of Gilts and Sows
Authors: Kellerman Cecilia , Malaluang Pongpreecha , Hansson Ingrid , Eliasson Selling Lena , Morrell Jane M.
Published: January 2022

Extenders for boar semen contain antibiotics, which may induce antimicrobial resistance (AMR) in inseminated females. The objective was to investigate AMR of bacteria isolated from the cervix of sows and gilts in standing heat, representing females previously exposed to antibiotics in the semen extender and non-exposed females, respectively. Cervical swabs were taken from 30 multiparous sows and 30 gilts prior to their first insemination. After culturing on agar plates, bacterial isolates were identified by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry and antimicrobial minimum inhibitory concentrations (MIC) were determined. Differences in antibiotic resistance between sows and gilts were analyzed by Chi-squared or Fisher’s exact test. Bacteria isolated were mostly Staphylococcus spp., Streptococcus spp. and Corynebacterium spp. Higher MICs were observed for isolates from sows than from gilts. Most (>80%) Corynebacterium spp. were resistant to clindamycin; small numbers (<20%) were resistant to gentamicin, penicillin, vancomycin, ciprofloxacin and rifampicin, with no differences between gilts and sows. Corynebacterium from gilts were more often resistant to tetracycline than those from sows (25% vs. 4.17%; p = 0.04). In conclusion, bacteria from the porcine cervix showed low resistance to most antibiotics except for clindamycin, but antibacterial resistance may increase with increasing parity.
Influence of Sodium Humate on the Growth Performance, Diarrhea Incidence, Blood Parameters, and Fecal Microflora of Pre-Weaned Dairy Calves
Authors: Wang Dong , You Zhendong , Du Yuanyi , Zheng Duo , Jia Haotian , Liu Yun
Published: January 2022

This study aimed to evaluate the effects of the administration of sodium humate (NaH) on the growth performance, diarrhea incidence, and fecal microflora of pre-weaned Holstein calves. In a 53-day experiment, forty healthy newborn female calves were randomly allocated to the following four treatment groups: (1) control (basal diet); (2) 1-gram NaH (basal diet extra orally supplemented with 1 g of NaH dissolved in 100 mL of milk or milk replacer daily); (3) 3-gram NaH (basal diet extra orally supplemented with 3 g of NaH dissolved in 100 mL of milk or milk replacer daily); and (4) 5-gram NaH (basal diet extra orally supplemented with 5 g of NaH dissolved in 100 mL of milk or milk replacer daily). NaH was mixed with milk (d 2–20) or milk replacer (d 21–53). Calves in the 5-gram NaH group had a higher ADG during d 1 to 21 and d 21 to 53 than the other groups did (p < 0.05). Fecal scores and diarrheal incidence were significantly lower in the 3-gram and 5-gram NaH groups than the 1-gram NaH and control groups during d 1 to 20 (p < 0.05). The serum IgA, IgG and IL-4 concentrations, and T-SOD and T-AOC activities were higher, and the serum IL-6, TNF-α, D-lactic acid, and MDA concentrations were lower in the 5-gram NaH group than the control group (p < 0.05). Furthermore, NaH supplementation increased the abundances of Bifidobacterium and Lactobacillus but decreased the abundance of Escherichia coli in feces (p < 0.05). These encouraging findings indicated that supplementation with 5 g of NaH effectively improved the immune status, antioxidant capacity, and intestinal beneficial bacteria, and further improved the growth performance and reduced the diarrhea incidence of the pre-weaned dairy calves.
Changes in Fatty Acids Profile, Health Indices, and Physical Characteristics of Organic Eggs from Laying Hens at the Beginning of the First and Second Laying Cycles
Authors: Zita Lukáš , Okrouhlá Monika , Krunt Ondřej , Kraus Adam , Stádník Luděk , Čítek Jaroslav , Stupka Roman
Published: January 2022

The present study compared the fatty acid profile and some physical parameters of eggs from hens reared according to the organic system at the beginning of the first and second laying cycle. A total of 1080 eggs were analysed at the beginning of the first (from the 28th to 30th week of age) and the second (from the 78th to 80th week of age) laying cycle. It was found that the hen ages influenced the egg weight, egg surface area, yolk proportion, and eggshell colour. Albumen and eggshell proportion, albumen, yolk index, Haugh unit score, and eggshell strength were lower in eggs from older hens compared with those produced from younger layers. Monounsaturated fatty acids were found in higher amounts than saturated fatty acids and polyunsaturated fatty acids in egg yolks of eggs from layers only at the beginning of the second laying cycle. The PUFAn-6/n-3 ratio, saturation, atherogenic, and thrombogenic indices were significantly lower in the egg yolks from older hens compared to younger layers. These findings (regarding the eggs from the older ones) prove that it is practical to utilize them in the organic farming system during a period of two years.
Early Pregnancy in Jennies in the Caribbean: Corpus Luteum Development and Progesterone Production, Uterine and Embryo Dynamics, Conceptus Growth and Maturation
Authors: Segabinazzi Lorenzo G. T. M. , Roberts Brandy N. , Peterson Erik W. , Ambrosia Rachael , Bergfelt Don , Samper Juan , French Hilari , Gilbert Robert O.
Published: January 2022

We aimed to characterize early embryo development and changes in corpus luteum (CL) development and progesterone profile in pregnant vs. non-pregnant jennies. Eight jennies were enrolled in the study. In the first two cycles, the jennies were monitored by transrectal ultrasonography and had blood harvested for hormone profile assay. In the third cycle, jennies were bred by a jack of proven fertility. Jennies were then monitored and sampled for up to 30 days of pregnancy. Data were evaluated by random-effects multiple linear regression, and correlations were expressed as Pearson’s correlation coefficient. Progesterone concentration rose rapidly from ovulation (D0) until D7, plateaued until D12–14, then precipitously declined between D14 and 15, remaining low until the next ovulation in non-pregnant cycles. In the pregnant jennies, the progesterone concentration rose to maximal concentrations on D7–11, being higher at this stage than in non-pregnant cycles, then declined gradually up to D30. In all cycles, the volume of the CL increased steadily until D6, when it plateaued in pregnant jennies. For non-pregnant jennies, CL volume decreased slowly from D6 to D11 and then had a faster drop. Uterine tone increased following ovulation, becoming turgid around the day of embryo fixation (D15.0 ± 0.9). An embryonic vesicle (EV) was first detected on D9.3 ± 0.5 (2.4 ± 0.5 mm). The EV remained spherical until D18.6 ± 1.4. The embryo proper was first detected ventrally in the vesicle on D20.8 ± 1.1 and the embryonic heartbeat by D22.0 ± 0.9. The allantoic sac was identified at D24.0 ± 0.9, and at D30, the allantoic sac filled the ventral half of the EV. This study provides evidence that higher cumulative concentrations of progesterone are correlated to size of the EV, and there were changes in the luteal dynamics and progesterone profiles in pregnant vs. non-pregnant jennies.
Resistance Patterns, mcr-4 and OXA-48 Genes, and Virulence Factors of Escherichia coli from Apennine Chamois Living in Sympatry with Domestic Species, Italy
Authors: Smoglica Camilla , Vergara Alberto , Angelucci Simone , Festino Anna Rita , Antonucci Antonio , Moschetti Lorenzo , Farooq Muhammad , Marsilio Fulvio , Di Francesco Cristina Esmeralda
Published: January 2022

The aim of this study was to determine and characterize potential resistance mechanisms against selected Critically Important Antibiotics in Escherichia coli isolates collected from wild and domestic ruminants living in the Maiella National Park, in Central Italy. A total of 38 isolates were obtained from red deer, Apennine chamois, cattle, sheep, and goats grazing in lands with different levels of anthropic pressure. Antimicrobial susceptibility was determined by Minimal Inhibitory Concentration testing, showing phenotypic resistance to colistin, meropenem, or ceftazidime in 9 isolates along with one bacterial strain being resistant to three of the tested antibiotics. In addition, the biomolecular assays allowed the amplification of the genes conferring the colistin (mcr-4), the carbapenems (OXA-48), penicillins and cephalosporins (TEM, SHV, CMY-1, CMY-2) resistance. In order to describe the potential pathogenicity of isolates under study, virulence genes related to Shiga toxin-producing (STEC) and enteropathogenic (EPEC) pathovars were identified. This study is the first report of mcr-4 and OXA-48 genes in resistant E. coli harboring virulence genes in Italian wildlife, with special regard to Apennine chamois and red deer species. The multidisciplinary approach used in this study can improve the early detection of emerging antibiotic resistance determinants in human-animal-environment interfaces by means of wildlife monitoring.
Long-Term Outcomes in Patients on Life-Long Antibiotics: A Five-Year Cohort Study
Authors: Kiss Christopher , Connoley Declan , Connelly Kathryn , Horne Kylie , Korman Tony , Woolley Ian , Lau Jillian S. Y.
Published: January 2022

Background: Little is known about the impacts at an individual level of long-term antibiotic consumption. We explored health outcomes of long-term antibiotic therapy prescribed to a cohort of patients to suppress infections deemed incurable. Methods: We conducted a 5-year longitudinal study of patients on long-term antibiotics at Monash Health, a metropolitan tertiary-level hospital network in Australia. Adults prescribed antibiotics for >12 months to suppress chronic infection or prevent recurrent infection were included. A retrospective review of medical records and a descriptive analysis was conducted. Results: Twenty-seven patients were followed up during the study period, from 29 patients originally identified in Monash Health in 2014. Seven of the 27 patients (26%) died from causes unrelated to the suppressed infection, six (22%) ceased long-term antibiotic therapy and two (7%) required treatment modification. Fifteen (56%) were colonised with multiresistant microorganisms, including vancomycin resistant Enterococci, methicillin resistant Staphylococcus aureus, and carbapenem resistant Enterobacteriaciae. Conclusions: This work highlights the potential pitfalls of long-term antibiotic therapy, and the frailty of this cohort, who are often ineligible for definitive curative therapy.
Antimicrobial Resistance and Environmental Health: A Water Stewardship Framework for Global and National Action
Authors: Kaiser Rachel A. , Taing Lina , Bhatia Himesh
Published: January 2022

Antimicrobial resistance (AMR) is a global health crisis that affects all life on Earth. In 2015, the World Health Organization developed guidance to combat AMR in accordance with a One Health framework considering human, animal, and environment sectors of planetary health. This study reviewed global guidance and 25 National Action Plans to evaluate thematic priorities in One Health AMR approaches using a novel framework that additionally facilitated the identification of water-related stewardship gaps, as water resources are recognized as the primary environmental AMR reservoir and dissemination pathway. This review found that global and national stewardship primarily focuses on mitigating antibiotic use in the human and animal sectors, overlooking environmental drivers, particularly diverse environmental waters. The findings of this study highlight the need to broaden the scope of water-related AMR concerns beyond water, sanitation, and hygiene (WASH) infrastructure for water supply and wastewater treatment, and account for environmental waters in AMR development and dissemination, particularly in low-income countries where half a billion people rely on environmental waters to meet daily needs. Equitably accounting for water environments, supplies, and waste in AMR prevention, mitigation, surveillance, and innovation can significantly enhance the integration of environmental objectives in One Health AMR stewardship.
Metabolic Adaptions/Reprogramming in Islet Beta-Cells in Response to Physiological Stimulators—What Are the Consequences
Authors: Newsholme Philip , Rowlands Jordan , Rose’Meyer Roselyn , Cruzat Vinicius
Published: January 2022

Irreversible pancreatic β-cell damage may be a result of chronic exposure to supraphysiological glucose or lipid concentrations or chronic exposure to therapeutic anti-diabetic drugs. The β-cells are able to respond to blood glucose in a narrow concentration range and release insulin in response, following activation of metabolic pathways such as glycolysis and the TCA cycle. The β-cell cannot protect itself from glucose toxicity by blocking glucose uptake, but indeed relies on alternative metabolic protection mechanisms to avoid dysfunction and death. Alteration of normal metabolic pathway function occurs as a counter regulatory response to high nutrient, inflammatory factor, hormone or therapeutic drug concentrations. Metabolic reprogramming is a term widely used to describe a change in regulation of various metabolic enzymes and transporters, usually associated with cell growth and proliferation and may involve reshaping epigenetic responses, in particular the acetylation and methylation of histone proteins and DNA. Other metabolic modifications such as Malonylation, Succinylation, Hydroxybutyrylation, ADP-ribosylation, and Lactylation, may impact regulatory processes, many of which need to be investigated in detail to contribute to current advances in metabolism. By describing multiple mechanisms of metabolic adaption that are available to the β-cell across its lifespan, we hope to identify sites for metabolic reprogramming mechanisms, most of which are incompletely described or understood. Many of these mechanisms are related to prominent antioxidant responses. Here, we have attempted to describe the key β-cell metabolic adaptions and changes which are required for survival and function in various physiological, pathological and pharmacological conditions.
Plant-Based Polyphenols: Anti-Helicobacter pylori Effect and Improvement of Gut Microbiota
Authors: Guerra-Valle María , Orellana-Palma Patricio , Petzold Guillermo
Published: January 2022

Helicobacter pylori (H. pylori) infection affects more than half of the world’s population, and thus, about 10 to 20% of people with H. pylori suffer from peptic ulcers, which may ultimately lead to gastric cancer. The increase in antibiotic resistance and susceptibility has encouraged the search for new alternative therapies to eradicate this pathogen. Several plant species are essential sources of polyphenols, and these bioactive compounds have demonstrated health-promoting properties, such as the gut microbiota stimulation, inflammation reduction, and bactericidal effect. Therefore, this review aims to discuss the potential effect of plant-based polyphenols against H. pylori and their role in the gut microbiota improvement.
The Response to Oxidative Damage Correlates with Driver Mutations and Clinical Outcome in Patients with Myelofibrosis
Authors: Genovese Elena , Mirabile Margherita , Rontauroli Sebastiano , Sartini Stefano , Fantini Sebastian , Tavernari Lara , Maccaferri Monica , Guglielmelli Paola , Bianchi Elisa , Parenti Sandra , Carretta Chiara , Mallia Selene , Castellano Sara , Colasante Corrado , Balliu Manjola , Bartalucci Niccolò , Palmieri Raffaele , Ottone Tiziana , Mora Barbara , Potenza Leonardo , Passamonti Francesco , Voso Maria Teresa , Luppi Mario , Vannucchi Alessandro Maria , Tagliafico Enrico , Manfredini Rossella ,   on behalf of the Mynerva (MYeloid NEoplasms Research Venture AIRC)
Published: January 2022

Myelofibrosis (MF) is the Philadelphia-negative myeloproliferative neoplasm characterized by the worst prognosis and no response to conventional therapy. Driver mutations in JAK2 and CALR impact on JAK-STAT pathway activation but also on the production of reactive oxygen species (ROS). ROS play a pivotal role in inflammation-induced oxidative damage to cellular components including DNA, therefore leading to greater genomic instability and promoting cell transformation. In order to unveil the role of driver mutations in oxidative stress, we assessed ROS levels in CD34+ hematopoietic stem/progenitor cells of MF patients. Our results demonstrated that ROS production in CD34+ cells from CALR-mutated MF patients is far greater compared with patients harboring JAK2 mutation, and this leads to increased oxidative DNA damage. Moreover, CALR-mutant cells show less superoxide dismutase (SOD) antioxidant activity than JAK2-mutated ones. Here, we show that high plasma levels of total antioxidant capacity (TAC) correlate with detrimental clinical features, such as high levels of lactate dehydrogenase (LDH) and circulating CD34+ cells. Moreover, in JAK2-mutated patients, high plasma level of TAC is also associated with a poor overall survival (OS), and multivariate analysis demonstrated that high TAC classification is an independent prognostic factor allowing the identification of patients with inferior OS in both DIPSS lowest and highest categories. Altogether, our data suggest that a different capability to respond to oxidative stress can be one of the mechanisms underlying disease progression of myelofibrosis.
Brassinosteroid Biosynthetic Gene SlCYP90B3 Alleviates Chilling Injury of Tomato (Solanum lycopersicum) Fruits during Cold Storage
Authors: Hu Songshen , Wang Tonglin , Shao Zhiyong , Meng Fanliang , Chen Hao , Wang Qiaomei , Zheng Jirong , Liu Lihong
Published: January 2022

Tomato is susceptible to chilling injury during cold storage. In this study, we found that low temperature promoted the expression of brassinosteroid (BR) biosynthetic genes in tomato fruits. The overexpression of SlCYP90B3 (SlCYP90B3-OE), a key BR biosynthetic gene, alleviated the chilling injury with decreased electrical conductivity and malondialdehyde. In SlCYP90B3-OE tomato fruits, the activities of antioxidant enzymes, including ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), were markedly increased, while the activity of membranous lipolytic enzymes, lipoxygenase (LOX), and phospholipase D (PLD), were significantly decreased when compared with the wild-type in response to cold storage. Furthermore, the expression level of the cold-response-system component, SlCBF1, was higher in SlCYP90B3-OE fruits than in the wild-type fruits. These results indicated that SlCYP90B3 might be involved in the chilling tolerance of tomato fruits during cold storage, possibly by regulating the antioxidant enzyme system and SlCBF1 expression.
Genetic Variation of a Lentil (Lens culinaris) Landrace during Three Generations of Breeding
Authors: Gleridou Anthoula , Tokatlidis Ioannis , Polidoros Alexios
Published: January 2022

Genetic differentiation between 40 lentil genotypes was tested using molecular markers. The genotypes were produced from a Greek landrace of commercial interest via the honeycomb breeding methodology, i.e., single-plant selection in the absence of competition, across three successive pedigree generations. The selected genotypes from each generation were examined for genetic relationships using 15 SSR molecular markers with HRM analysis. As expected, low variation among consecutive generations at the level of 2.5–7.7% was detected. Analysis of molecular variance (AMOVA) revealed that partitioning of this variation was at higher percentage within each generation’s population than between them. Population structure analysis indicated that ongoing selection could effectively shift the allelic composition in each generation. The applied honeycomb breeding methodology that effectively improved progeny yield and seed quality increased the percentage of favorable alleles altering allelic composition but not eliminating genetic variation of the breeding population.
Essential Oil Variability of Azorean Cryptomeriajaponica Leaves under Different Distillation Methods, Part 1: Color, Yield and Chemical Composition Analysis
Authors: Arruda Filipe , Rosa José , Rodrigues Ana , Oliveira Luísa , Lima Ana , Barroso José , Lima Elisabete
Published: January 2022

This study mainly deals with the effect of hydrodistillation (HD) and water-steam distillation (WSD) methods on the color, yield, and chemical profile of the essential oil (EO) from Cryptomeria japonica fresh leaves from São Miguel Island (Azores Archipelago, Portugal). The yields of EO–HD (pale-yellowish) and EO–WSD (colorless) samples were 1.21% and 0.45% (v/w), respectively. The GC–FID, GC–MS, and 13C-NMR analyses of EO–HD vs. EO–WSD revealed (i) a high-content of monoterpenes (72.8% vs. 86.7%), mainly α-pinene (34.5% vs. 46.4%) and sabinene (20.2% vs. 11.6%), and oxygenated mono- and sesquiterpenes (20.2% vs. 9.6%); (ii) similar sesquiterpene (1.6% vs. 1.6%), β-myrcene (5.9% vs. 5.8%), and camphene (3.5% vs. 3.8%) contents; and (iii) significant differences in other classes/components: EO–HD is richer in oxygenated sesquiterpenes (17.1%, mainly elemol (10.4%) and α-eudesmol (3.4%)) and diterpenes (3%; mostly phyllocladene), while EO–WSD is richer in oxygenated monoterpenes (7.2%, mainly terpinen-4-ol (5.4%)), p-cymene (4.4%), and limonene (3.2%). Overall, the color, yield, and quantitative composition of the EO samples studied are strongly influenced by the distillation method. Nonetheless, this C. japonica leaf EO displayed a consistent α-pinene- and sabinene-rich composition. The same chemotype was found in a commercial Azorean C. japonica leaf EO sample, obtained by industrial steam distillation (SD), as well as in Corsica C. japonica leaf EO–HD. Furthermore, the bioactive composition of our EO samples revealed the potential to be used in green plant protection and in the medical, food, cosmetic, and household industries.
MUFFLE: Multi-Modal Fake News Influence Estimator on Twitter
Authors: Wu Cheng-Lin , Hsieh Hsun-Ping , Jiang Jiawei , Yang Yi-Chieh , Shei Chris , Chen Yu-Wen
Published: January 2022

To alleviate the impact of fake news on our society, predicting the popularity of fake news posts on social media is a crucial problem worthy of study. However, most related studies on fake news emphasize detection only. In this paper, we focus on the issue of fake news influence prediction, i.e., inferring how popular a fake news post might become on social platforms. To achieve our goal, we propose a comprehensive framework, MUFFLE, which captures multi-modal dynamics by encoding the representation of news-related social networks, user characteristics, and content in text. The attention mechanism developed in the model can provide explainability for social or psychological analysis. To examine the effectiveness of MUFFLE, we conducted extensive experiments on real-world datasets. The experimental results show that our proposed method outperforms both state-of-the-art methods of popularity prediction and machine-based baselines in top-k NDCG and hit rate. Through the experiments, we also analyze the feature importance for predicting fake news influence via the explainability provided by MUFFLE.
Analysis and Comparison of Electromagnetic Microrobotic Platforms for Biomedical Applications
Authors: Chen Ruipeng , Folio David , Ferreira Antoine
Published: January 2022

Magnetic microrobotics is a promising technology for improving minimally invasive surgery (MIS) with the ambition of enhancing patient care and comfort. The potential benefits include limited incisions, less hemorrhaging and postoperative pain, and faster recovery time. To achieve this, a key issue relies on the design of a proper electromagnetic actuation (EMA) setup which is based on the use of magnetic sources. The magnetic field and its gradient generated by the EMA platform is then used to induce magnetic torque and force for microrobot manipulations inside the human body. Like any control systems, the EMA system must be adapted to the given controlled microrobot and customized for the application. With great research efforts on magnetic manipulating of microrobots, the EMA systems are approaching commercial applications, and their configurations are becoming more suitable to be employed in real medical surgeries. However, most of the proposed designs have not followed any specific rule allowing to take into account the biomedical applications constraints. Through reviewing the different proposed EMA systems in the literature, their various specifications and configurations are comprehensively discussed and analyzed. This study focus on EMA platforms that use electromagnets. From this review and based on the biomedical application specifications, the appropriate EMA system can be determined efficiently.
Quantifying Nutrient Content in the Leaves of Cowpea Using Remote Sensing
Authors: Amaral Julyanne Braga Cruz , Lopes Fernando Bezerra , Magalhães Ana Caroline Messias de , Kujawa Sebastian , Taniguchi Carlos Alberto Kenji , Teixeira Adunias dos Santos , Lacerda Claudivan Feitosa de , Queiroz Thales Rafael Guimarães , Andrade Eunice Maia de , Araújo Isabel Cristina da Silva , Niedbała Gniewko
Published: January 2022

Although hyperspectral remote sensing techniques have increasingly been used in the nutritional quantification of plants, it is important to understand whether the method shows a satisfactory response during the various phenological stages of the crop. The aim of this study was to quantify the levels of phosphorus (P), potassium (K), calcium (Ca) and zinc (Zn) in the leaves of Vigna Unguiculata (L.) Walp using spectral data obtained by a spectroradiometer. A randomised block design was used, with three treatments and twenty-five replications. The crop was evaluated at three growth stages: V4, R6 and R9. Single-band models were fitted using simple correlations. For the band ratio models, the wavelengths were selected by 2D correlation. For the models using partial least squares regression (PLSR), the stepwise method was used. The model showing the best fit was used to estimate the phosphorus content in the single-band (R² = 0.62; RMSE = 0.54 and RPD = 1.61), band ratio (R² = 0.66; RMSE = 0.65 and RPD = 1.52) and PLSR models, using data from each of the phenological stages (R² = 0.80; RMSE = 0.47 and RPD = 1.66). Accuracy in modelling leaf nutrients depends on the phenological stage, as well as the amount of data used, and is more accurate with a larger number of samples.
Non-Centrifugal Sugar (NCS) and Health: A Review on Functional Components and Health Benefits
Authors: Zidan Dina , Azlan Azrina
Published: January 2022

Non-centrifugal sugar (NCS) is the scientific term the Food and Agriculture Organization (FAO) uses to define a solid product, produced by sugarcane juice evaporation, which is unrefined or minimally refined. NCS is referred to in various names globally, the most significant ones are whole cane sugar, panela (Latin America), jaggery (India) and kokuto (Japan). NCS contains minerals, bioactive compounds, flavonoids and phenolic acids, which have therapeutic potentials from time immemorial. Even though the bioactive property is dependent on the composition, which relies mainly on the agronomic conditions and production process, NCS possesses antioxidant and anti-inflammatory properties. Hence, substituting the consumption of refined sugar with NCS might be helpful in the control of chronic diseases generally connected to oxidative stress and inflammation. Experimental facts from in vitro and in vivo models have proven that NCS plays an essential role in weight management, maintaining insulin sensitivity and preventing neurodegenerative diseases. NCS has also shown hypoglycemic and hypolipidemic effects. This review aims to synopsize the recent literature pertaining to the benefits of NCS in human health. The NCS can be considered a nutraceutical and functional food. However, detailed and regulated studies are important to enhance the beneficial effects in human and animal interventions.
Evaluation of Electric Vehicle Integrated Charging Safety State Based on Fuzzy Neural Network
Authors: Gao Hui , Zang Binbin , Sun Lei , Chen Liangliang
Published: January 2022

Electric vehicles have been promoted worldwide because of their high energy efficiency and low pollution. However, frequent charging safety accidents have to a certain extent restricted the development of electric vehicles. Therefore, it is extremely important to accurately evaluate the safety state of EV charging. The paper presents an integrated safety assessment method for electric vehicle charging safety based on fuzzy neural network. The integrated fault model was established by analyzing the correlation between truck–pile–grid. Then the integrated evaluation index was analyzed and sorted out, and the comprehensive fuzzy evaluation method used to evaluate. Following this, the improved GA_BP neural network algorithm was used to calculate the weight. Compared with the evaluation effect before and after the improvement, the simulation results show that the GA_BP neural network has higher accuracy and smaller error than the ordinary BP neural network. Finally, the feasibility and effectiveness of the evaluation method was verified by a case study.
Molecular Identification of Keratinase DgokerA from Deinococcus gobiensis for Feather Degradation
Authors: Meng Yong , Tang Yin , Zhang Xiuhong , Wang Jin , Zhou Zhengfu
Published: January 2022

Keratin is a tough fibrous structural protein that is difficult to digest with pepsin and trypsin because of the presence of a large number of disulfide bonds. Keratin is widely found in agricultural waste. In recent years, especially, the development of the poultry industry has resulted in a large accumulation of feather keratin resources, which seriously pollute the environment. Keratinase can specifically attack disulfide bridges in keratin, converting them from complex to simplified forms. The keratinase thermal stability has drawn attention to various biotechnological industries. It is significant to identify keratinases and improve their thermostability from microorganism in extreme environments. In this study, the keratinases DgoKerA was identified in Deinococcus gobiensis I-0 from the Gobi desert. The amino acid sequence analysis revealed that DgoKerA was 58.68% identical to the keratinase MtaKerA from M. thermophila WR-220 and 40.94% identical to the classical BliKerA sequence from B. licheniformis PWD-1. In vitro enzyme activity analysis showed that DgoKerA exhibited an optimum temperature of 60 °C, an optimum pH of 7 and a specific enzyme activity of 51147 U/mg. DgoKerA can degrade intact feathers at 60 °C and has good potential for industrial applications. The molecular modification of DgoKerA was also carried out using site-directed mutagenesis, in which the mutant A350S enzyme activity was increased by nearly 30%, and the results provide a theoretical basis for the development and optimization of keratinase applications.
Microbial Depolymerization of Epoxy Resins: A Novel Approach to a Complex Challenge
Authors: Pardi-Comensoli Lucrezia , Tonolla Mauro , Colpo Andrea , Palczewska Zuzanna , Revikrishnan Sharanya , Heeb Markus , Brunner Ivano , Barbezat Michel
Published: January 2022

The objective of this project is evaluating the potential of microbes (fungi and bacteria) for the depolymerization of epoxy, aiming at the development of a circular management of natural resources for epoxy in a long-term prospective. For depolymerization, epoxy samples were incubated for 1, 3, 6 and 9 months in soil microcosms inoculated with Ganoderma adspersum. Contact angle data revealed a reduction in the hydrophobicity induced by the fungus. Environmental scanning electron microscopy on epoxy samples incubated for more than 3 years in microbiological water revealed abundant microbiota. This comprised microbes of different sizes and shapes. The fungi Trichoderma harzianum and Aspergillus calidoustus, as well as the bacteria Variovorax sp. and Methyloversatilis discipulorum, were isolated from this environment. Altogether, these results suggest that microbes are able to colonize epoxy surfaces and, most probably, also partially depolymerize them. This could open promising opportunities for the study of new metabolisms potentially able depolymerize epoxy materials.
Impact of Magnetic-Pulse and Chemical-Thermal Treatment on Alloyed Steels’ Surface Layer
Authors: Kostyk Kateryna , Kuric Ivan , Saga Milan , Kostyk Viktoriia , Ivanov Vitalii , Kovalov Viktor , Pavlenko Ivan
Published: January 2022

The relevant problem is searching for up-to-date methods to improve tools and machine parts’ performance due to the hardening of surface layers. This article shows that, after the magnetic-pulse treatment of bearing steel Cr15, its surface microhardness was increased by 40–50% compared to baseline. In this case, the depth of the hardened layer was 0.08–0.1 mm. The magnetic-pulse processing of hard alloys reduces the coefficient of microhardness variation from 0.13 to 0.06. A decrease in the coefficient of variation of wear resistance from 0.48 to 0.27 indicates the increased stability of physical and mechanical properties. The nitriding of alloy steels was accelerated 10-fold that of traditional gas upon receipt of the hardened layer depth of 0.3–0.5 mm. As a result, the surface hardness was increased to 12.7 GPa. Boriding in the nano-dispersed powder was accelerated 2–3-fold compared to existing technologies while ensuring surface hardness up to 21–23 GPa with a boride layer thickness of up to 0.073 mm. Experimental data showed that the cutting tool equipped with inserts from WC92Co8 and WC79TiC15 has a resistance relative to the untreated WC92Co8 higher by 183% and WC85TiC6Co9—than 200%. Depending on alloy steel, nitriding allowed us to raise wear resistance by 120–177%, boriding—by 180–340%, and magneto-pulse treatment—by more than 183–200%.
Seismic Analysis of Moderate Size Earthquakes Recorded on Stations at Close Epicentral Distances
Authors: Sinadinovski Cvetan , Markušić Snježana , Stanko Davor , McCue Kevin F. , Pekevski Lazo
Published: January 2022

In this study, we analyzed the near-field seismic records of two moderate sized earthquakes in the Western Balkan region: the September 2016 Skopje earthquake, magnitude ML5.3 and the March 2020 Zagreb earthquake, magnitude ML5.5. Such recordings at close epicentral distances are rare and are thus very useful for testing some of the theoretical assumptions used in modeling earthquake risk. Firstly, response spectra were computed using the digital time histories for the three closest stations to the Skopje 2016 earthquake and the two closest stations to the Zagreb 2020 earthquake. Their characteristics were examined in terms of frequency and peak amplitude ranges. Secondly, the Nakamura method was applied to the records from the selected five stations coded SKO, FCE, IZIIS, QUHS, and QARH. The results of the spectral analysis were compared with interpretations from the geological and geotechnical maps at each location. Our findings support the idea that these combined methods can be used to categorize the underlying structural profile to a first approximation and can be used to derive velocity models.
Study on Behavior of Some Perennial Flowering Species Used in Vertical Systems for Green Facades in Eastern European Climate
Authors: Cojocariu Mirela , Chelariu Elena Liliana , Chiruţă Ciprian
Published: January 2022

The negative effects of urbanization such as urban overheating, pollution, high population density and so on are being experienced by city inhabitants more than ever, thus motivating a high number of researchers to find viable solutions to mitigate these effects. The present paper aims to identify an assortment of ornamental plants appropriate to be successfully used in various facade covering systems for buildings located in Eastern European countries. For this purpose, throughout a vegetation season, the project’s team thoroughly monitored the percentage of survival, the coverage degree, and the behavior (biometric aspects and visual quality) of ten flowering species planted vertically and being oriented towards all four cardinal points. At the same time, the team conducted a thorough monitoring of some parameters of the soil (pH, humidity, and temperature) and of the outside environment (light intensity and temperature). Two experimental structures were built, insulated on the inside and covered at the top with thermal insulating panels, to simulate the same conditions of an insulated and unheated construction. The monitoring results showed that Heuchera x hybrida ‘Fire Alarm’, Heuchera x hybrida ‘Marmalade’ and Festuca galuca had a healthy appearance throughout the year, regardless of the cardinal orientation while providing a good coverage of the vertical surface.
Automated Prediction of Extraction Difficulty and Inferior Alveolar Nerve Injury for Mandibular Third Molar Using a Deep Neural Network
Authors: Lee Junseok , Park Jumi , Moon Seong Yong , Lee Kyoobin
Published: January 2022

Extraction of mandibular third molars is a common procedure in oral and maxillofacial surgery. There are studies that simultaneously predict the extraction difficulty of mandibular third molar and the complications that may occur. Thus, we propose a method of automatically detecting mandibular third molars in the panoramic radiographic images and predicting the extraction difficulty and likelihood of inferior alveolar nerve (IAN) injury. Our dataset consists of 4903 panoramic radiographic images acquired from various dental hospitals. Seven dentists annotated detection and classification labels. The detection model determines the mandibular third molar in the panoramic radiographic image. The region of interest (ROI) includes the detected mandibular third molar, adjacent teeth, and IAN, which is cropped in the panoramic radiographic image. The classification models use ROI as input to predict the extraction difficulty and likelihood of IAN injury. The achieved detection performance was 99.0% mAP over the intersection of union (IOU) 0.5. In addition, we achieved an 83.5% accuracy for the prediction of extraction difficulty and an 81.1% accuracy for the prediction of the likelihood of IAN injury. We demonstrated that a deep learning method can support the diagnosis for extracting the mandibular third molar.