bluuebunny/crossref_metadata_2025 huggingface dataset:
Hypocholesterolaemia and mortality in patients with coronary artery disease
Authors: Ndrepepa Gjin , Holdenrieder Stefan , Cassese Salvatore , Xhepa Erion , Fusaro Massimiliano , Kastrati Adnan
Published: December 2019

Background

The association between hypocholesterolaemia and outcome of patients with coronary artery disease (CAD) remains controversial. We undertook this study to investigate whether there is an association between spontaneous or under statin therapy occurring hypocholesterolaemia and mortality in patients with CAD.

Materials and Methods

This study included 14 952 patients with CAD undergoing percutaneous coronary intervention (PCI). Hypocholesterolaemia was defined as a total cholesterol (TC) <157 mg/dL (the upper limit of 1st quintile of TC). The study outcome was all‐cause mortality at 30 days and 3 years after PCI.

Results

Patients are categorized in four groups according to TC and statin therapy on admission: statin‐naïve patients with hypocholesterolaemia (n = 1102), statin‐naïve patients without hypocholesterolaemia (n = 7490), statin‐treated patients with hypocholesterolaemia (n = 1824) and statin‐treated patients without hypocholesterolaemia (n = 4536). In these groups, 30‐day all‐cause deaths occurred in 3.7%, 1.4%, 1.2% and 0.6% of the patients, respectively; 3‐year deaths occurred in 18.0%, 8.4%, 10.9% and 7.2%, of the patients, respectively. After adjustment, hypocholesterolaemia remained independently associated with 30‐day (adjusted hazard ratio [HR] = 1.50, 95% confidence interval [CI] 1.07 to 2.09; P < 0001) and 3‐year (HR = 1.29 [1.12‐1.47]; P < .001) mortality. Statin therapy on admission was independently associated with 30‐day (HR = 0.61 [0.43‐0.86]; P = .012) and 3‐year (HR = 0.82 [0.72‐0.94]; P = .017) mortality with no statin‐by‐cholesterol interaction with respect to 30‐day (adjusted Pint = 0.669) or 3‐year (adjusted Pint = 0.767) all‐cause mortality suggesting that statins reduce the risk of mortality irrespective of cholesterol level.

Conclusions

In patients with CAD, hypocholesterolaemia on admission was independently associated with increased risk of all‐cause mortality at 30 days and 3 years after PCI.
Management of maxillofacial trauma in the elderly: A European multicenter study
Authors: Brucoli Matteo , Boffano Paolo , Romeo Irene , Corio Chiara , Benech Arnaldo , Ruslin Muhammad , Forouzanfar Tymour , Starch‐Jensen Thomas , Rodríguez‐Santamarta Tanía , de Vicente Juan Carlos , Snäll Johanna , Thorén Hanna , Tarle Marko , Dediol Emil , Pechalova Petia , Pavlov Nikolai , Daskalov Hristo , Doykova Iva , Kelemith Kadri , Tamme Tiia , Kopchak Andrey , Shumynskyi Ievgen , Corre Pierre , Bertin Helios , Goguet Quentin , Anquetil Marine , Louvrier Aurélien , Meyer Christophe , Dovšak Tadej , Vozlič David , Birk Anže , Aničić Boban , Konstantinovic Vitomir S.
Published: December 2019

Background/Aims

Management of maxillofacial trauma in the geriatric population poses a great challenge due to anatomical variations and medical comorbidities. The aim of this study was to analyze the management variables, timing, and outcomes of facial fractures in elderly patients (aged 70 years or more) at several European departments of oral and maxillofacial surgery.

Materials and Methods

This study was based on a systematic computer‐assisted database that allowed the recording of data from all geriatric patients with facial fractures from the involved maxillofacial surgical units across Europe between 2013 and 2017.

Results

A total of 1334 patients were included in the study: 665 patients underwent closed or open surgical treatment. A significant association (P < .005) was found between the presence of concomitant injuries and a prolonged time between hospital admission and treatment. The absence of indications to treatment was associated with comorbidities and an older age (P < .000005).

Conclusions

Elderly patients require specific attention and multidisciplinary collaboration in the diagnosis and sequencing of trauma treatment. A prudent attitude may be kept in selected cases, especially when severe comorbidities are associated and function is not impaired.
A review of potential factors promoting fish movement in inter‐basin water transfers, with emergent patterns from a trait‐based risk analysis for a large‐scale project in china
Authors: Schmidt Bjorn V. , Wang Zhen , Ren Peng , Guo Chuanbo , Qin Jiao , Cheng Fei , Xie Songguang
Published: December 2019

Inter‐basin water transfers create new pathways between previously disjunct systems and communities. If fish movement occurs, it can lead to invasion or altered regional connectivity patterns, which could induce biotic/genetic homogenisation or synchronisation. Understanding ecological factors promoting movement is critical for predicting potential impacts during project planning, to assess ongoing effects, and to develop mediation strategies. Potential characteristics influencing movement rates were reviewed, including intrinsic traits that increase passive entrainment or active dispersal rates, extrinsic traits of the connected environments and their interactions. In order to examine patterns among these potentially influencing characteristics, a trait‐based screening method for movement and invasion risk was developed to analyse linked communities in a database from a large‐scale inter‐basin water diversion in China. Groups of fishes that scored high in the risk assessment were compared with worldwide examples of entrainment in inter‐basin water transfer systems or smaller‐scale diversions to examine emergent patterns of shared ecological characteristics. Specific traits that were most often shared in these groups, indicating a higher likelihood of movement in water diversions, include the following: a smaller body size, high adult or larval abundance, migratory behaviour, generalist and pelagic habitat preferences, and a high reproductive output. The synthesis of factors identified in this research can be used to guide future empirical studies to fill knowledge gaps regarding ecological impacts of inter‐basin water transfers or other smaller‐scale diversions on local communities and ecosystems.
Co‐incorporation of Chinese milk vetch (Astragalus sinicus L.) and rice (Oryza sativa L.) straw minimizes CH4 emissions by changing the methanogenic and methanotrophic communities in a paddy soil
Authors: Zhou Guopeng , Gao Songjuan , Xu Changxu , Zeng Naohua , Rees Robert M. , Cao Weidong
Published: December 2019

The practice of co‐incorporating rice straw and leguminous green manure (such as Chinese milk vetch) into paddy soils has become increasingly popular in the last 10 years, although its effect on soil CH4 emissions and microbial community structure is poorly understood. In this study, data from a 2‐year pot experiment were used to reveal the effects of the co‐incorporation of rice straw and Chinese milk vetch on CH4 emissions and methanogenic and methanotrophic communities in paddy soil. Five treatments were chosen: CK (unamended control), CF (inorganic fertilizer), FM (inorganic fertilizer and Chinese milk vetch, 20 g 10 kg−1 dry soil, 4.5 t ha−1), FR (inorganic fertilizer and rice straw, 20 g 10 kg−1 dry soil, 4.5 t ha−1) and FMR (inorganic fertilizer and Chinese milk vetch and rice straw mixture, 5 + 15 g 10 kg−1 dry soil, 1.125 + 2.375 t ha−1). Overall, the soil CH4 emissions in the 2 years under all residue treatments (104.4–122.8 and 83.3–133.3 g CH4 m−2) were higher than those in CK (57.0 and 20.7 g CH4 m−2) and CF (71.8 and 21.9 g CH4 m−2) treatments. The FMR treatment decreased CH4 emissions when compared with FM and FR, especially in the second year. A higher abundance of methanogens (mcrA gene copies) was found under the plant residue‐treated soils (4.35 × 108 g−1 dry soil on average) and FM increased the abundance of methanotrophs (pmoA gene copies, 2.68 × 106 g−1 dry soil). Application of plant residues changed the methanogenic and methanotrophic community structures in comparison with CF (e.g., markedly increased the populations of Methanobacteriales and Methylomonas and suppressed Methanomicrobiales and Methylobacter populations). Methane emissions were significantly and positively related to the mcrA and pmoA gene copy numbers, as well as the mcrA/pmoA ratio. The partial least squares path model (PLS‐PM) indicated that the rice plant biomass (path coefficient = 0.64), the abundance of methanogens (path coefficient = 0.57) and the community structure of methanotrophs (path coefficient = −0.59) were determinants of CH4 emissions. Among the three residue‐treated soils, FMR showed the lowest CH4 emissions and was associated with a slight decrease in the mcrA/pmoA ratio and a significant increase in the Type I/Type II methanotrophs ratio. Thus, we recommend the co‐incorporation of Chinese milk vetch and rice straw as a sustainable fertilization pattern to improve the growth of rice and minimize CH4 emissions.

Highlights

Residue application improves rice productivity (except for FR), soil fertility and CH4 emissions.

Co‐incorporation of MV and RS reduces CH4 emissions in comparison with pure MV or RS addition.

Residue application changes the community structure of methanogens and methanotrophs.

The methanogenic abundance and the methanotrophic community structure determine the CH4 emissions.
Minimum fatigue striation spacing and its stress amplitude dependence in a commercially pure titanium
Authors: Ahmed Mansur , Islam Md Saiful , Yin Shuo , Lupoi Rocco
Published: December 2019

We report the fatigue striation spacing of commercially pure titanium with respect to stress amplitude. Rotating bending fatigue tests were performed at 150, 175, 200 and 227 MPa in which samples did not fail at 150 MPa. No fatigue striation up to a certain crack length is observed for the studied conditions. For each condition, a minimum striation spacing value is found that decreases from 0.45 to 0.36 to 0.24 μm with increasing stress from 175 to 200 to 227 MPa, respectively. The minimum striation spacing remains constant up to a certain crack length for each condition. Thereafter, it starts increasing with crack length. It is quite understandable that the constant striation spacing over a certain crack length may not represent the macroscopic fatigue crack growth rate. The fatigue crack growth mechanism during the constant striation spacing region has been discussed in detail.
The risk of individual fish being captured multiple times in a catch and release fishery
Authors: Thorstad Eva B. , Diserud Ola H. , Solem Øyvind , Havn Torgeir B. , Bjørum Lars Oftedal , Kristensen Torstein , Urke Henning Andre , Johansen Martin R. , Lennox Robert J. , Fiske Peder , Uglem Ingebrigt
Published: December 2019

The proportion of angled Atlantic salmon Salmo salar L. being caught and released has increased. If individuals are repeatedly captured, this may have fish welfare consequences. Of 995 Atlantic salmon tagged during catch and release in eight Norwegian rivers, 10% were captured twice, while 3% were captured three times within the same fishing season. The probability that released salmon were captured again decreased with decreasing time left of the fishing season, decreased for larger‐sized fish and varied among rivers/years. Increased exploitation rates within the river, indicating an increased fishing pressure, strongly increased the probability that fish would be recaptured. However, the proportion of salmon caught a second time was much lower than the total exploitation rates in the same rivers (which was on average 46%). For fish tagged in the sea, the likelihood of being angled decreased with time since entering the river, which may explain why the recapture rates of caught and released fish were lower than the total exploitation rates.
Early generation hybrids may drive range expansion of two invasive fishes
Authors: Coulter Alison A. , Brey Marybeth K. , Lamer James T. , Whitledge Gregory W. , Garvey James E.
Published: December 2019

Introgressive hybridisation between two invasive species has the potential to contribute to their invasion success and provide genetic resiliency to rapidly adapt to new environments. Additionally, differences in the behaviour of hybrids may lead to deleterious ecosystem effects that compound any negative impacts of the invading parental species.

Invasive silver carp (Hypophthalmichthys molitrix) and bighead carp (Hypophthalmichthys nobilis) provide an opportunity to evaluate how hybridisation may influence the behaviour, dispersal, and spread of an invasive species introgressive complex. In order to investigate the role hybrids may have in the invasion ecology of bigheaded carps, we examined the distribution, movements, and environmental cues for movement of two invasive fishes and their hybrids in the Illinois River (U.S.A.).

Early generation hybrids (e.g. F1,F2, and first‐generation backcross individuals) composed a greater proportion of the population at the invasion front where abundances of bigheaded carp were low. A greater proportion of early hybrids passed through dams upstream towards the invasion front than did other hybrids and parental species.

The movements and environmental cues for movement of late‐generation backcrosses (more genetically similar to parental genotype) were not different from the parental species with which they shared the most alleles. Although the direction of the relationship between movement and environment was sometimes different for the parental species and associated advanced generation hybrids, these results indicate that management for parental species will also affect most hybrids.

Although early generation hybrids are rare, our results indicate they may disperse towards low‐density population zones (i.e. invasion fronts) or are produced at greater frequency in low‐density areas. These rare hybrids have the potential to produce a variety of unique genetic combinations that could result in more rapid adaptation of a non‐native population to their invaded range potentially facilitating the establishment of invasive species.
Soil carbon increased by twice the amount of biochar carbon applied after 6 years: Field evidence of negative priming
Authors: Blanco‐Canqui Humberto , Laird David A. , Heaton Emily A. , Rathke Samuel , Acharya Bharat Sharma
Published: December 2019

Applying biochar to agricultural soils has been proposed as a means of sequestering carbon (C) while simultaneously enhancing soil health and agricultural sustainability. However, our understanding of the long‐term effects of biochar and annual versus perennial cropping systems and their interactions on soil properties under field conditions is limited. We quantified changes in soil C concentration and stocks, and other soil properties 6 years after biochar applications to corn (Zea mays L.) and dedicated bioenergy crops on a Midwestern US soil. Treatments were as follows: no‐till continuous corn, Liberty switchgrass (Panicum virgatum L.), and low‐diversity prairie grasses, 45% big bluestem (Andropogon gerardii), 45% Indiangrass (Sorghastrum nutans), and 10% sideoats grama (Bouteloua curtipendula), as main plots, and wood biochar (9.3 Mg/ha with 63% total C) and no biochar applications as subplots. Biochar‐amended plots accumulated more C (14.07 Mg soil C/ha vs. 2.25 Mg soil C/ha) than non‐biochar‐amended plots in the 0–30 cm soil depth but other soil properties were not significantly affected by the biochar amendments. The total increase in C stocks in the biochar‐amended plots was nearly twice (14.07 Mg soil C/ha) the amount of C added with biochar 6 years earlier (7.25 Mg biochar C/ha), suggesting a negative priming effect of biochar on formation and/or mineralization of native soil organic C. Dedicated bioenergy crops increased soil C concentration by 79% and improved both aggregation and plant available water in the 0–5 cm soil depth. Biochar did not interact with the cropping systems. Overall, biochar has the potential to increase soil C stocks both directly and through negative priming, but, in this study, it had limited effects on other soil properties after 6 years.
Measuring the impact of changing from standard half‐life (SHL) to extended half‐life (EHL) FVIII prophylaxis on health‐related quality of life (HRQoL) in boys with moderate/severe haemophilia A: Lessons learned with the CHO‐KLAT tool
Authors: Carcao Manuel , Zunino Laura , Young Nancy L. , Dover Saunya , Bouskill Vanessa , Hilliard Pamela , Price Victoria E. , Blanchette Victor S.
Published: December 2019

Introduction

In many countries, there is a shift from standard half‐life (SHL) to extended half‐life (EHL) clotting factor concentrates (CFCs).

Aim

To describe the experience of switching from SHL to an EHL FVIII CFC and the impact of this on frequency of infusions, factor consumption, bleeding rates and HRQoL using the Canadian Hemophilia Kids’ Life Assessment Tool (CHO‐KLAT).

Methods

A retrospective chart review was conducted at a single haemophilia treatment centre in 2018 that included boys (ages: 4‐18 years) with moderate/severe haemophilia A, without inhibitors, who switched from a SHL to an EHL FVIII CFC in the previous 2 years and for whom HRQoL data were available.

Results

The study cohort comprised 38 boys [mean (SD) age: 11.0 (3.4) years] with moderate (n = 5)/severe (n = 33) haemophilia A. The switch was associated with a 33% reduction in the number of weekly infusions from a median of 3.5 to 2.3 (P < .0001) and a 17% reduction in median FVIII consumption from 103 IU/kg/wk to 85.5 IU/kg/wk (P = .004). There was no significant change in annualized joint bleed rates or in CHO‐KLAT scores.

Conclusions

Despite documenting several benefits of switching to EHL FVIII (less infusions, lower factor consumption with no increase in bleeding), our study did not demonstrate any improvement in HRQoL. We conclude that either the current CHO‐KLAT tool is not optimized to measure burden of treatment administration in boys with low bleed rates switching from SHL to EHL FVIII CFCs or that a reduction of 1.2 infusions/week does not result in a meaningful change in HRQoL.
HIV‐1 genotype is independently associated with immunodeficiency progression among Chinese men who have sex with men: an observational cohort study
Authors: Liang Y , Han Z , Shui J , Cheng W , Zhong F , Cai Q , Wang H , Wu H , Xu H , Tang S
Published: December 2019

Objectives

HIV‐1 genetic diversity is increasing among men who have sex with men (MSM) in China, but the association of HIV‐1 genotype with disease progression remains to be elucidated.

Methods

We collected data in an observational longitudinal cohort study of 860 HIV‐1‐infected MSM in Guangzhou, China between January 2008 and March 2017. Kaplan‐Meier analysis and Cox proportional hazard model were used to predict the time from HIV‐1 diagnosis to immunodeficiency progression (CD4 cell count < 200 cells/μl) as well as adjusted hazard ratio (aHR).

Results

CRF01_AE and HIV‐1 subtype B infection were associated with higher percentage of patients progressed to immunodeficiency and higher incidence of immunodeficiency than infection with CRF07_BC or CRF55_01B. Compared with CRF07_BC, the time from HIV‐1 diagnosis to immunodeficiency were different among the major HIV‐1 genotypes, which ranked as follows, in descending order: CRF07_BC (7.03 years) > CRF55_01B (5.71 years, P = 0.014; aHR 3.752, P = 0.0923) > CRF01_AE (5.18 years, P < 0.001; aHR 4.733, P = 0.0152). HIV‐1 genotype, viral load and baseline CD4 T‐cell count were three independent variables associated with disease progression.

Conclusions

Our results confirm differential rates of immunodeficiency progression as a function of HIV‐1 genotype. The impact of HIV‐1 genotype on HIV epidemics, patient management and prevention should be further investigated.
Consumer debt attitudes: The role of gender, debt knowledge and skills
Authors: Białowolski Piotr , Cwynar Andrzej , Cwynar Wiktor , Węziak‐Białowolska Dorota
Published: December 2019

The link between financial attitudes and consumer financial market behaviour is well documented. However, little is known about the role of financial knowledge and skills—the main components of the financial literacy construct—in shaping debt attitudes. This link is especially absent from the gender perspective. This study focuses on consumer debt literacy and debt attitudes. A representative sample of adult Poles (N = 1,004) was participated in a computer‐assisted telephone interview. Latent class analysis was employed to reveal their debt attitudes, and subsequently, links between debt literacy and debt attitudes were studied with multinomial regression models. The results show that respondents in Poland can be grouped into five classes exhibiting different attitude profiles. The structure of debt attitudes is complex and differs from a simple unidimensional pro‐debt/ anti‐debt construct. Although this study did not find significant gender differences either in the conceptualization of debt attitudes or in their drivers, the results showed that in all but one class, consumer attitudes are strongly linked to either debt knowledge or debt skills or to both. Debt skills were revealed to be a particularly strong predictor of consumer debt attitudes.
Healthcare professionals’ perceptions of the implementation of the transitional discharge model for community integration of psychiatric clients
Authors: Forchuk Cheryl , Martin Mary‐Lou , Sherman Deborrah , Corring Deborah , Srivastava Rani , O'Regan Tony , Gyamfi Sebastian , Harerimana Boniface
Published: December 2019

Research has demonstrated the benefits of mental healthcare interventions that ensure a safe transition of clients throughout the discharge and community integration process. This paper reports on qualitative data from focus groups with health professionals collected as part of a larger a mixed method study designed to examine the effectiveness and sustainability of implementing the transitional discharge model. Data collection involved two sets of focus groups, which were held at six months and one‐year post‐implementation. There were 216 health professional participants from nine (9) hospitals across the Province of Ontario, Canada. Data analysis used a four‐step ethnographic framework by Leininger (1985) to identify descriptors and recurrent and major themes. The study identified four major themes, including healthcare professionals’ roles and positive experiences in implementing the transitional discharge model; perceived benefits of the model; challenges to implementing the model; and suggestions for sustaining the model's implementation. Healthcare professionals felt that the implementation of the transitional discharge model has the potential for increasing their awareness of the process of clients’ integration, serving as a framework for discharge planning, and reducing hospital readmissions. The study findings may provide healthcare providers with information on pragmatic ways to plan clients’ discharge, to bridge the gap between hospital and community care, and to positively impact client health outcomes.
Primary vs delayed primary closure in patients undergoing lower limb amputation following trauma: A randomised control study
Authors: Katiyar Anand K. , Agarwal Harshit , Priyadarshini Pratyusha , Kumar Abhinav , Kumar Subodh , Gupta Amit , Mishra Biplab , Aggarwal Richa , Soni Kapil D. , Mathur Purva , Sagar Rajesh , Srivastava Anurag , Banerjee Niladri , Sagar Sushma
Published: December 2019

Lower limb crush injury is a major source of mortality and morbidity in trauma patients. Complications, especially surgical site infections (SSIs) are a major source of financial burden to the institute and to the patient as it delays rehabilitation. As such, every possible attempt should be made to reduce any complications. We, thus, aimed to compare the outcomes in early vs delayed closure of lower extremity stumps in cases of lower limb crush injury requiring amputation, so as to achieve best possible outcome. A randomised controlled study was conducted in the Division of Trauma Surgery & Critical Care at Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi from 1 September 2018 to 30 June 2019 and included patients undergoing lower limb amputation below hip joint. Patients were randomised in two groups, in one group amputation stump was closed primarily, while in the second group delayed primary closure of stump was performed. We compared rate of SSI, length of hospital stay, and number of surgeries in both the groups. Fifty‐six patients with 63 amputation stumps were recruited in the study. Mean age of patients in the study was 34 years, of which about 95% patients were males. The most common mechanism of injury was road traffic injury in 66% of patients. Mean injury severity score was 12.28 and four patients had diabetes preoperatively. Total 63 extremities were randomised with 30 cases in group I and 33 cases in group II as per computer‐generated random number. Above knee amputations was commonest (57.14%) followed by below knee amputations (33.3%). Two patients died in the current study. In group I, In‐hospital infection was detected in 7 cases (23.3%) and in group II 9 cases (27.3%) had SSI during hospital admission (P > .05). Mean hospital stay in group I was 10.32 ± 7.68 days and in group II was 11 ± 8.17 days (P > .05). Road traffic injuries and train‐associated injuries are a major cause of lower limb crush injuries, leading to limb loss. Delayed primary closure of such wounds requires extra number of surgical interventions than primary closure. There is no difference in extra number of surgical interventions required in both the groups. Thus, primary closure can be safely performed in patients undergoing lower limb amputations following trauma, provided that a good lavage and wound debridement is performed.
On the “Invisible Inventory Conundrum” in RFID‐Equipped Supply Chains: A Data Science Approach to Assessing Tag Performance
Authors: Rao Shashank , Ellis Scott C. , Goldsby Thomas J. , Raju Dheeraj
Published: December 2019

Recent trade reports suggest that RFID implementation continues to lag lofty projections. A primary concern is that, despite the high cost of implementing RFID systems, realized read‐rates fall short of expectations. This results in the invisible inventory conundrum whereby tagged merchandise may still not be accurately represented in inventory records. Drawing from data science to address this issue, we ask: How can directed data mining models be used to identify laboratory test performance criteria for RFID tags that operate reliably across the idiosyncratic facilities (i.e., unique DCs, warehouses, and stores) that comprise apparel retailers’ supply chains? We investigate this question by advancing a methodology that integrates laboratory test performance data, field tests of RFID tags fixed to apparel items and scanned under normal operating conditions, and the application of five directed data mining models to the integrated data set of laboratory and field test results. Our analyses of 45,416 observations show that two directed data mining models may identify—with near‐100% accuracy—laboratory test criteria that discriminate tags having 99% or greater read‐rates in the field. Accordingly, our study validates a generalizable methodology for identifying technical performance standards for tags that operate reliably within apparel retailers’ supply chains.
Exploring the contributing factors to workarounds to the hospital information system in Malaysian hospitals
Authors: Salahuddin Lizawati , Ismail Zuraini , Abd Ghani Mohd Khanapi , Mohd Aboobaider Burhanuddin , Hasan Basari Abd Samad
Published: December 2019

Objectives

The objective of this study was to identify the factors influencing workarounds to the Hospital Information System (HIS) in Malaysian government hospitals.

Methods

Semi‐structured interviews were conducted among 31 medical doctors in three Malaysian government hospitals on the implementation of the Total Hospital Information System (THIS) between March and May 2015. A thematic qualitative analysis was performed on the resultant data to deduce the relevant themes.

Results

Five themes emerged as the factors influencing workarounds to the HIS: (a) typing skills, (b) system usability, (c) computer resources, (d) workload, and (e) time.

Conclusions

This study provided the key factors as to why doctors were involved in workarounds during the implementation of the HIS. It is important to understand these factors in order to help mitigate work practices that can pose a threat to patient safety.
Effects of coating pistachio kernels with mixtures of whey protein and selected herbal plant extracts on growth inhibition of Aspergillus flavus and prevention of aflatoxin during storage
Authors: Tavakolipour Hamid , Kalbasi‐Ashtari Ahmad , Mokhtarian Mohsen
Published: December 2019

Whey protein concentrate (WPC) mixed with various concentrations of Shirazi thyme (ST), sage, and cumin seed (CS) extracts separately. Then pistachio kernels (PK) contaminated with Aspergillus flavus (Af) were coated with each extract and the Af mycelium and generated aflatoxins measured after 3, 5, and 7‐days at 20°C. The ST, sage, and CS had two major antioxidants of thymol (~27%) and carvacrol (~41%), α‐thujone (~28%) and camphor (~14%), and cumin‐aldehyde (~21%) and safranal (~20%), respectively. While the Af mycelium diameter on PK without extract became >90 mm within 3 days, it was shrunk after 7 days when the WPC‐coated PK had 4,000 ppm ST extract. When ST concentrations increased in WPC‐coated PK linearly, the Af growth and aflatoxins production decreased logarithmically. No aflatoxins (B1, B2, G1, and G2) detected in PK after 9 days when the extract concentrations of ST, sage, and CS in WPC reached, respectively to 5,000, 4,500, and 6,500 ppm. Sage extract had significantly (p < .01) the highest TPC (lowest IC50) and preventing power for aflatoxin generation in comparison with ST and CS extracts. The PK will be safe and healthy if the extract concentration of sage, ST, and CS exceed 950, 1,400, and 1,700 mg/kg, respectively.
Salmonella sensitivity to sodium hypochlorite and citric acid in washing water of lettuce residues
Authors: Borges Thaís J. , Moretti Letícia K. , Silva Marselle M. N. , Tondo Eduardo C. , Pereira Karen S.
Published: December 2019

Salmonella spp. is one of the main lettuce pathogens and should be inactivated during the disinfection of these vegetables before consumption. In minimally processed vegetable industries, residues of organic matter can prevent the inactivation of this pathogen by disinfectants. The objective of the present work was to evaluate the inactivation of Salmonella isolated from organic lettuce to sodium hypochlorite (25 and 50 ppm) and citric acid (0.5 and 1%) in washing water added with lettuce residues. To do so, a washing water with lettuce residues was elaborated, and Salmonella was added in the order of 106 CFU/ml. Thereafter, each sanitizer was added separately to evaluate its effect on reducing Salmonella counts. After 1, 2, 3, 4, 5, 10, and 15 min of contact with the sanitizers, serial dilutions using neutralizer (0.5% sodium thiosulfate) were performed and each dilution was sown in Xylose‐Lysine‐Desoxycholate medium. Total aerobic mesophilic counts of wash water with lettuce residues before testing (without Salmonella) and after 15 min of exposure to each sanitizer (with Salmonella) were also performed. In addition, the free chlorine still present in the samples after the contact of sodium hypochlorite with lettuce residues for 15 min. The results demonstrated that 50 and 25 ppm sodium hypochlorite could reduce 6 log CFU/ml of Salmonella in 1 and 3 min of contact, respectively, while 0.5 and 1% citric acid was able to reduce 1.26 and 1.74 log CFU/ml respectively from the same microorganism within 15 min of contact. The total aerobic mesophilic counts of the wash water before being tested were, on average, 1.5 log CFU/ml. After addition of Salmonella, with 15 min of contact with the sanitizer, the results of total counts showed the same magnitude as the Salmonella counts. Organic matter may have reacted with the free chlorine present, reducing chlorine concentrations, since values of 30.4 ppm were observed when the initial concentration should be 50 and 17.1 ppm when the initial concentration should be 25 ppm. Based on the results, sodium hypochlorite demonstrated a greater microbial reduction capacity in wash water with lettuce residues, indicating that it is more appropriate to avoid cross‐contamination between batches during sanitation of lettuce in washing tanks.
Immunofluorescence analysis of sensory nerve endings in the interosseous membrane of the forearm
Authors: Rein Susanne , Esplugas Mireia , Garcia‐Elias Marc , Magin Thomas M. , Randau Thomas M. , Siemers Frank , Philipps Hubertus M.
Published: December 2019

The human interosseous membrane (IOM) is a fundamental stabilizer during forearm rotation. To investigate the dynamic aspects of forearm stability, we analyzed sensory nerve endings in the IOM. The distal oblique bundle (DOB), the distal accessory band (DAB), the central band (CB), the proximal accessory band (PAB), the dorsal oblique accessory cord (DOAC) and the proximal oblique cord (POC) were dissected from 11 human cadaver forearms. Sensory nerve endings were analyzed at two levels per specimen as total cell amount/mm2 after immunofluorescence staining with low‐affinity neurotrophin receptor p75, protein gene product 9.5, S‐100 protein and 4′,6‐diamidino‐2‐phenylindole on an Apotome microscope, according to Freeman and Wyke’s classification. Sensory nerve endings were significantly more commonly found to be equally distributed throughout the structures, rather than being epifascicular, interstitial, or close to the insertion into bone (P ≤ 0.001, respectively). Free nerve endings were the predominant mechanoreceptor in all six structures, with highest density in the DOB, followed by the POC (P ≤ 0.0001, respectively). The DOB had the highest density of Pacini corpuscles. The DOAC and CB had the lowest amounts of sensory innervation. The high density of sensory corpuscles in the DOB, PAB and POC indicate that proprioceptive control of the compressive and directional muscular forces acting on the distal and proximal radioulnar joints is monitored by the DOB, PAB and POC, respectively, due to their closed proximity to both joints, whereas the central parts of the IOM act as structures of passive restraint.
Baseline sensitivity of Fusarium graminearum from wheat fields in Henan, China, to metconazole and analysis of cross resistance with carbendazim and phenamacril
Authors: Liu Shengming , Liu Jinliang , Fu Liuyuan , Chen Jinpeng , Wang Shuan , Jiang Jia , Che Zhiping , Tian Yuee , Chen Genqiang
Published: December 2019

The filamentous plant pathogenic fungus Fusarium graminearum is one of the most important pathogens causing Fusarium head blight (FHB) in wheat in the Henan Province of China. Metconazole is among the demethylation inhibitor (DMI) fungicides with a higher inhibitory activity on the mycelial growth of F. graminearum. In 2016 and 2017, 119 single spore isolates of F. graminearum, prior to being exposed to metconazole, were recovered from 52 wheat fields near 11 cities in Henan Province. The inhibitory activity of metconazole on the mycelia of the Henan F. graminearum population was determined, and EC50 values were calculated. The range of EC50 values of the Henan F. graminearum population to metconazole was 0.0103 to 0.0775 μg/ml with an average EC50 value of 0.0293 ± 0.0114 μg/ml. The sensitivity frequency distribution curve presented a single peak in a narrow range. No cross‐resistance was found between the DMI fungicide metconazole and the benzimidazole fungicide carbendazim or the cyanoacrylate fungicide phenamacril. Therefore, these sensitivity data could be used as the baseline of F. graminearum susceptibility to metconazole in the Henan Province and provide the basis for monitoring metconazole resistance in this area.
Self-assembled liposome from core-sheath chitosan-based fibres for buccal delivery of carvedilol: formulation, characterization and in vitro and ex vivo buccal absorption
Authors: Chen Jianting , Pan Hao , Duan Hongliang , Deng Wenbin , Zhang Fei , Yang Xinggang , Pan Weisan
Published: December 2019

Objectives

A novel drug delivery system based on self-assembled liposome from core-sheath nanofibres for buccal delivery of Carvedilol (Car) was explored.

Methods

The Car-loaded PVP/PC (phospholipids) layer was coated with chitosan-PVA (CS-PVA) or CS-PVP to increase retention period in the mouth. SEM, confocal laser scanning microscopy (CLSM), XRD and Fourier transform infrared spectroscopy were applied to characterize fibre diameter and drug state. Appearance, particle size and encapsulation efficiency of self-assembled liposome were investigated by transmission electron microscopy (TEM) and Zeta-sizer Nano. The dissolution test and permeation tests across porcine buccal mucosa and TR146 cell model also were run.

Key findings

Confocal laser scanning microscopy and XRD confirmed the core-sheath structure of coaxial fibre and non-crystalline form of Car, separately. TEM demonstrated the sphere morphology of self-assembled liposome from spun fibres after contacting water. The dissolution test implied the ratio of PC to Car had a huge impact on drug release. The permeation tests across porcine buccal mucosa and TR146 cell model showed similar result, namely our formulation having a better permeation performance than Car suspension. The indirect toxicity against TR146 cells presented 5 mg/ml (or lower) of fibre extraction was safe for cells.

Conclusions

These researches exhibited this drug delivery system was promising and advantageous for Car buccal delivery.
Congruent spatial patterns of species richness and phylogenetic diversity in karst flora: Case study of Primulina (Gesneriaceae)
Authors: Xu Mei‐Zhen , Yang Li‐Hua , Kong Hang‐Hui , Wen Fang , Kang Ming
Published: December 2019

The karst landform in southern China is renowned for its high levels of species diversity and endemism. Globally, karst ecosystems are under threat from unsustainable anthropogenic disturbance and climate changes and are among the most threatened ecosystems worldwide. In this study, we used the typical karst endemic genus in southern China, Primulina Hance, as a model to identify areas within the karst landform with high diversity and to investigate congruence between phylogenetic and species‐based measures of diversity. Using phylogenetic information and species distribution data, we measured geographical patterns of diversity with four metrics: species richness (SR), corrected weighted endemism (CWE), phylogenetic diversity (PD), and phylogenetic endemism (PE). Our results revealed a high spatial congruence among SR, PD, and PE, with hotspot areas identified in the Nanling Mountains (i.e., north Guangdong and northeast Guangxi) and southeast Yungui Plateau (i.e., north and southwest Guangxi), whereas the hotspots of CWE are comparatively uniform throughout the geographic extent. The categorical analysis of neo‐ and paleoendemism identified a pattern of mixed neo‐ and paleoendemism in numerous grid cells, suggesting that karst areas in southern China have acted as both “museums” and “cradles” of plant evolution. Conservation gap analysis of hotspots revealed that the majority of prioritized hotspots (>90%) of the genus are outside of protected areas, therefore indicating the limited effectiveness of national nature reserves for the karst flora. Overall, our results suggest that the karst flora merits more conservation attention and SR can be an effective surrogate to capture PD in conservation planning.
Speciation history of European (Anguilla anguilla) and American eel (A. rostrata), analysed using genomic data
Authors: Nikolic Natacha , Liu Shenglin , Jacobsen Magnus W. , Jónsson Bjarni , Bernatchez Louis , Gagnaire Pierre‐Alexandre , Hansen Michael M.
Published: December 2019

Speciation in the ocean could differ from terrestrial environments due to fewer barriers to gene flow. Hence, sympatric speciation might be common, with American and European eel being candidates for exemplifying this. They show disjunct continental distributions on both sides of the Atlantic, but spawn in overlapping regions of the Sargasso Sea from where juveniles are advected to North American, European and North African coasts. Hybridization and introgression are known to occur, with hybrids almost exclusively observed in Iceland. Different speciation scenarios have been suggested, involving either vicariance or sympatric ecological speciation. Using RAD sequencing and whole‐genome sequencing data from parental species and F1 hybrids, we analysed speciation history based on the joint allele frequency spectrum (JAFS) and pairwise sequentially Markovian coalescent (PSMC) plots. JAFS supported a model involving a split without gene flow 150,000–160,000 generations ago, followed by secondary contact 87,000–92,000 generations ago, with 64% of the genome experiencing restricted gene flow. This supports vicariance rather than sympatric speciation, likely associated with Pleistocene glaciation cycles and ocean current changes. Whole‐genome PSMC analysis of F1 hybrids from Iceland suggested divergence 200,000 generations ago and indicated subsequent gene flow rather than strict isolation. Finally, simulations showed that results from both approaches (JAFS and PSMC) were congruent. Hence, there is strong evidence against sympatric speciation in North Atlantic eels. These results reiterate the need for careful consideration of cases of possible sympatric speciation, as even in seemingly barrier‐free oceanic environments palaeoceanographic factors may have promoted vicariance and allopatric speciation.
Too Many Cooks Spoil the Broth: Toward a Theory for How the Tragedy of the Anticommons Emerges in Organizations
Authors: McCarter Matthew W. , Kopelman Shirli , Turk Thomas A. , Ybarra Candace E.
Published: December 2019

In organizations, conflict revolves around the use of shared resources. Research on property rights, territoriality, and social dilemmas suggests that to reduce such conflict, organizations could facilitate the psychological privatization of commons resources. We introduce a model that helps understand how psychologically privatizing organizational commons resources—to prevent the overuse problem of the tragedy of the commons (Hardin, G. Science, 162, 1968, 1243)—can lead to the emergence of another resource dilemma. We develop a model that illustrates how resource complexity and group complexity increase psychological marking and defending behaviors. These behaviors potentially lead to a problem of resource underuse—a tragedy of the anticommons (Heller, M. A. Harvard Law Review, 111, 1998, 621)—in organizational settings. The conceptual model, integrating insights from research on property rights, territoriality, and social dilemmas with law and social psychology, provides a bottom‐up behavioral explanation of the emergence of the tragedy of the anticommons in organizations and outlines opportunities for future research.
A clinicopathological study of ALS with L126S mutation in the SOD1 gene presenting with isolated inferior olivary hypertrophy
Authors: Hideshima Makoto , Beck Goichi , Yamadera Misaki , Motoyama Yuichi , Ikenaka Kensuke , Kakuda Keita , Tsuda Hiroshi , Nagano Seiichi , Fujimura Harutoshi , Morii Eiichi , Murayama Shigeo , Mochizuki Hideki
Published: December 2019

We report an autopsy case of amyotrophic lateral sclerosis with L126S mutation in the superoxide dismutase 1 (SOD1) gene (SOD1). The patient was a 69‐year‐old Japanese man without relevant family history, who initially presented with slow progressive muscle weakness of the lower extremities without upper motor neuron signs, and died of respiratory failure 6 years after the onset. Neuropathological examination revealed a loss of lower motor neurons and degeneration of Clarke's column commensurate with that of the posterior spinocerebellar tract and the middle root zone of the posterior column. The primary motor area was minimally affected. Characteristic SOD1‐immunopositive neuronal intracytoplasmic inclusions, mixed with neurofilament accumulation, were present in the affected areas. Isolated inferior olivary hypertrophy was observed, but did not involve the contralateral dentate nucleus, or the ipsilateral red nucleus and central tegmental tract, where no neuronal inclusions were found. In combination with data from a previous autopsy case, this study suggests that the L126S mutation may cause focal neuronal degeneration in the brainstem.
Brachypodium distachyon MAP20 functions in metaxylem pit development and contributes to drought recovery
Authors: Smertenko Tetyana , Turner Glenn , Fahy Deirdre , Brew‐Appiah Rhoda A. T. , Alfaro‐Aco Raymundo , de Almeida Engler Janice , Sanguinet Karen A. , Smertenko Andrei
Published: December 2019

Pits are regions in the cell walls of plant tracheary elements that lack secondary walls. Each pit consists of a space within the secondary wall called a pit chamber, and a modified primary wall called the pit membrane. The pit membrane facilitates transport of solutions between vessel cells and restricts embolisms during drought. Here we analyzed the role of an angiosperm‐specific TPX2‐like microtubule protein MAP20 in pit formation using Brachypodium distachyon as a model system.

Live cell imaging was used to analyze the interaction of MAP20 with microtubules and the impact of MAP20 on microtubule dynamics. MAP20‐specific antibody was used to study expression and localization of MAP20 in different cell types during vascular bundle development. We used an artificial microRNAs (amiRNA) knockdown approach to determine the function of MAP20.

MAP20 is expressed during the late stages of vascular bundle development and localizes around forming pits and under secondary cell wall thickenings in metaxylem cells. MAP20 suppresses microtubule depolymerization; however, unlike the animal TPX2 counterpart, MAP20 does not cooperate with the γ‐tubulin ring complex in microtubule nucleation. Knockdown of MAP20 causes bigger pits, thinner pit membranes, perturbed vasculature development, lower reproductive potential and higher drought susceptibility.

We conclude that MAP20 may contribute to drought adaptation by modulating pit size and pit membrane thickness in metaxylem.
Effect of Particle Size Ratios on the Physical and Chemical Properties of Surgical‐Grade Calcium Sulfate Hemihydrate
Authors: Hu Pan , Lun Deng‐xing , Wang Peng‐sheng , Tu Zhi‐ming
Published: December 2019

Objectives

To analyze the optimum particle size or formula ratio of surgical‐grade calcium sulfate (CS) for appropriate compressive strength, setting time, and vitro degradation rate.

Methods

Three types of calcium sulfate hemihydrate (CSH) particles with diameters of 0–37.5 μm, 37.5–75 μm, and >75 μm were screened. Based on formulation ratio of different particles, this topic is divided into 10 groups by the unconstrained third‐order simplex lattice mixing design scheme in formula design experiment. The optimum formulation ratio of particle diameter for compressive strength, solidification time, and degradation rate in vitro was analyzed.

Results

When the percentage of the particle diameter of CS with 0–37.5 μm, 37.5–75 μm and >75 μm are 55.0%, 17.4%, and 27.6% respectively, the compressive strength of the test sample is the highest, which is 14.16 MPa. When the percentage of the particle diameter of CS with 0–37.5 μm, 37.5–75 μm, and >75 μm are 0.00%, 0.00%, and 100.00% respectively, the initial setting time of the sample is the longest, which is 410.0 s. When the percentage of the particle diameter of CS with 0–37.5 μm, 37.5–75 μm, and >75 μm are 0.00%, 0.00%, and 100.00% respectively, the final setting time of the sample is the largest, and the final setting time of the sample is 460.00 s. When the percentage of the particle diameter of CS with 0–37.5 μm, 37.5–75 μm, and >75 μm are 0.00%, 0.00%, and 100.00% respectively, the degradation rate of the sample in vitro is the slowest, which is 18.8%.

Conclusion

The morphological structure of surgical‐grade CS can affect compressive strength, setting time, and in vitro degradation rate. Surgical CS should be prepared based on different uses.