bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of CFTR Mutation Spectrum in Ethnic Russian Cystic Fibrosis Patients
Authors: Petrova Nika V. , Kashirskaya Nataliya Y. , Vasilyeva Tatyana A. , Kondratyeva Elena I. , Zhekaite Elena K. , Voronkova Anna Y. , Sherman Victoria D. , Galkina Varvara A. , Ginter Eugeny K. , Kutsev Sergey I. , Marakhonov Andrey V. , Zinchenko Rena A.
Published: May 2020

The distribution and frequency of the CFTR gene mutations vary considerably between countries and ethnic groups. Russians are an East Slavic ethnic groups are native to Eastern Europe. Russians, the most numerous people of the Russian Federation (RF), make about 80% of the population. The aim is to reveal the molecular causes of CF in ethnic Russian patients as comprehensively as possible. The analysis of most common CFTR mutations utilized for CF diagnosis in multiethnic RF population accounts for about 83% of all CF-causing mutations in 1384 ethnic Russian patients. Variants c.1521_1523delCTT (F508del), c.54-5940_273+10250del21kb (CFTRdele2,3), c.2012delT (2143delT), c.2052_2053insA (2184insA), and c.3691delT (3821delT) are most typical for CF patients of Russian origin. DNA of 154 CF patients, Russian by origin, in whom at least one mutant allele was not previously identified (164 CF alleles), was analyzed by Sanger sequencing followed by the multiplex ligase-dependent probe amplification (MLPA) method. In addition to the 29 variants identified during the previous test for common mutations, 91 pathogenic CFTR variants were also revealed: 29 missense, 19 nonsense, 14 frame shift in/del, 17 splicing, 1 in frame ins, and 11 copy number variations (CNV). Each of the 61 variants was revealed once, and 17 twice. Each of the variants c.1209G>C (E403D), c.2128A>T (K710X), c.3883delA (4015delA), and c.3884_3885insT (4016insT) were detected for three, c.1766+1G>A (1898+1G>A) and c.2834C>T (S945L) for four, c.1766+1G>C (1898+1G>C) and c.(743+1_744-1)_(1584+1_1585-1)dup (CFTRdup6b-10) for five, c.2353C>T (R785X) and c.4004T>C (L1335P) for six, c.3929G>A (W1310X) for seven, c.580-1G>T (712-1G>T for eight, and c.1240_1244delCAAAA (1365del5) for 11 unrelated patients. A comprehensive analysis of CFTR mutant alleles with sequencing followed by MLPA, allowed not only the identification of 163 of 164 unknown alleles in our patient sample, but also expansion of the mutation spectrum with novel and additional frequent variants for ethnic Russians.
Granger Causality Analysis of Geophysical, Geodetic and Geochemical Observations during Volcanic Unrest: A Case Study in the Campi Flegrei Caldera (Italy)
Authors: Tripaldi Simona , Scippacercola Sergio , Mangiacapra Annarita , Petrillo Zaccaria
Published: May 2020

The recent signs of reawakening at Campi Flegrei caldera (Southern Italy) received a great deal of attention due to the issues related to the volcanic risk management in a densely populated area. This paper explores relations between ground deformations, seismicity and geochemical time series in the time span 2004–2016. The aim is to unravel primary processes of unrest and the related indicators which may change in time. Data structure and interactions among variables were examined applying the clustering analysis, the correlations and the Granger causality test. The hierarchical agglomerative clustering detected two sub-periods which were further investigated. In both sub-period causal links were observed between variables while correlations did not appear and vice versa. Thus, well established formal approaches are required to study causal relations. Granger test results indicate that during 2004–2011 the awakening unrest could be mainly ascribed to hydrothermal system pressure fluctuations, probably induced by deep-rooted fluids injection, and that ground deformation together with CO2/H2O appears the most suitable geo-indicators. The 2011–2016 sub-period is characterized by enhanced dynamical connectivity. Granger test results suggest that the unrest is driven by a more localized and shallower thermohydromechanical engine. CO/CO2, He/CH4 and ground deformation velocity are mutually interacting appearing the most suitable geo-indicators.
COVID-19 and Gastrointestinal Symptoms—A Case Report
Authors: Mackett Alistair J. , Keevil Victoria L.
Published: May 2020

COVID-19, a new illness secondary to a novel Coronavirus emerged in December 2019 in China. Our early understanding of the clinical features of COVID-19 has been based on case series emerging from the first outbreak in Wuhan. These features included fever, a dry cough, myalgia and dyspnea. Gastrointestinal symptoms were rarely reported as a key feature. We present a case report of a 74-year-old male who presented with symptoms of gastroenteritis and subsequently tested positive for COVID-19. This article aims to highlight an uncommon presentation of COVID-19 and that a high index of suspicion is required for COVID-19 in older people given their greater likelihood of presenting atypically.
Adsorption of Lead (II) from Aqueous Solution with High Efficiency by Hydrothermal Biochar Derived from Honey
Authors: Wang Bo , Yu Jie , Liao Hui , Zhu Wenkun , Ding Pingping , Zhou Jian
Published: May 2020

A novel natural honey hydrothermal biochar (HHTB) was prepared using natural honey as raw material. The as-prepared adsorbent was applied to adsorb Pb2+ from aqueous solution and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy to investigate the structure and morphology change of the adsorbent before and after Pb2+ adsorption. The influence of the pH, initial Pb2+ concentration, temperature, and contact time on the adsorption of Pb2+ was systematically investigated. The results revealed that the adsorption capacity for Pb2+ is up to 133.2 mg·g−1 at initial pH of 5.0 and adsorption temperature of 298 K. Meanwhile, the adsorption of Pb2+ on HHTB can be well fitted by the pseudo-second-order model and Langmuir isotherm model. The adsorbent had great selectivity for Pb2+ from the aqueous solution containing coexisting ions including Cd2+, Co2+, Cr3+, Cu2+, Ni2+ and Zn2+. Furthermore, the adsorption of Pb2+ on HHTB was attributed to complexation coordination, where it involved hydroxyl and carboxylic groups on HHTB in the process of adsorption of Pb2+.
Review of the Lithuanian Alcohol Control Legislation in 1990–2020
Authors: Miščikienė Laura , Goštautaitė Midttun Nijolė , Galkus Lukas , Belian Gražina , Petkevičienė Janina , Vaitkevičiūtė Justina , Štelemėkas Mindaugas
Published: May 2020

Since the early 1990s, Lithuania has experienced an increasing level of alcohol consumption and a heavy burden of alcohol-related harm, which is associated with the development of alcohol policies. The aim of this analysis was to provide a chronology of change of Lithuanian alcohol control legislation and to present several other detailed examples of the political processes. The data were collected using document reviews. During the last three decades, the Lithuanian alcohol control policies have undergone several cycles of stricter control and liberalizations. Some of the limitations of the study are the exceptional focus on the public health perspective and the inclusion of policies targeting the population as a whole. The strength of the study is in providing a detailed background for future policy effectiveness studies. Some of the recent periods when a series of ‘best buy’ interventions were implemented during a short period are of particular importance, constituting a natural experiment, whose effects need to be studied in more detail in the future.
Measuring Regional Eco-Efficiency in China (2003–2016): A “Full World” Perspective and Network Data Envelopment Analysis
Authors: Ren Weizhen , Zhang Zilong , Wang Yueju , Xue Bing , Chen Xingpeng
Published: May 2020

Eco-efficiency enhancement is an inherent requirement of green development and an important indicator of high-quality development in general. It aims to achieve the coordinated development of nature, the economy, and society. Therefore, eco-efficiency measurements should focus on not only total factor input, but also process analysis. Based on the “full world” model in ecological economic theory, this study constructed a theoretical framework for a composite economic-environmental-social system that reflects human welfare and sustainability. To this end, using network data envelopment analysis (DEA), this study established a staged eco-efficiency evaluation model that uses economic, environmental, and social factors to measure the overall and staged eco-efficiency of China’s provinces from 2003 to 2016 and analyze its spatiotemporal characteristics. A geographically weighted regression (GWR) model was also used to analyze the influencing factors of eco-efficiency changes and the spatial differentiation in their effect intensity. The findings were as follows: (1) China’s overall eco-efficiency is still at a low level. It varies significantly from region to region, and only three regions are at the frontier of production. The eastern region has the highest eco-efficiency, followed by the central region, and the gap between the central and western regions has gradually narrowed. In terms of staged efficiency, the level of eco-efficiency in the production stage is less than in the environmental governance stage, which is less than that in the social input stage. (2) In terms of the efficiency of each stage, the efficiency level of the production stage showed a downward trend throughout the entire process, and the decline in the central and western regions was more obvious. The social input stage and the environmental governance stage both showed upward trends. The social input stage showed a higher level, and the increase was relatively flat during the period of study. Efficiency continued to rise during the environmental governance stage from 2003 to 2010 and rose overall, but with some fluctuations from 2011 to 2016. (3) Geographically weighted regression showed that the effects of the influencing factors on eco-efficiency had obvious spatial heterogeneity. The factors affecting overall, production stage, and social input eco-efficiency were, in order of effect intensity from high to low, economic growth level, marketization level, and social input level. In terms of environmental governance, social input level had the greatest impact, followed by economic growth; marketization level did not show a significant impact.
Aligning the “Manifesto for a European Research Network into Problematic Usage of the Internet” with the Diverse Needs of the Professional and Consumer Communities Affected by Problematic Usage of Pornography
Authors: Mead Darryl , Sharpe Mary
Published: May 2020

The Manifesto for a European research network into Problematic Usage of the Internet was published in May 2018. It was written from the perspective of the COST Action Network, a programme of the European Cooperation in Science and Technology CA16207 and is expected to have significant influence on research funding priorities over the next decade. The Manifesto identified nine key research priorities to advance understanding in the field. Our analysis shows that while at the most general level it identified problematic usage of pornography (PUP) as a key research priority, it then barely mentioned it again within the body of the report. This paper uses the Manifesto’s framework to suggest research areas into the problematic usage of pornography which are of particular relevance to clinicians and other professionals working in the field who want to develop approaches to assist individuals and target groups affected by PUP. It also looks at potential research opportunities inspired by the lived-experience of users withdrawing from PUP. A large number of opportunities are identified for new work on PUP across all nine key research areas of the Manifesto.
Bioethical Decisions in Neonatal Intensive Care: Neonatologists’ Self-Reported Practices in Greek NICUs
Authors: Dagla Maria , Petousi Vasiliki , Poulios Antonios
Published: May 2020

This study presents, for the first time, empirical data on practices regarding bioethical decision-making in treatment of preterm and ill newborns in Greece. The aim of the study was to: (a) record self-reported practices and involvement of Greek physicians in decisions of withholding and withdrawing neonatal intensive care, and (b) explore the implication of cultural, ethical, and professional parameters in decision-making. Methods: 71 physicians, employed fulltime in all public Neonatal Intensive Care Units (NICUs) (n = 17) in Greece, completed an anonymous questionnaire between May 2009 and May 2011. Results: One-third of the physicians in our sample admitted that they have, at least once in the past, decided the limitation of intensive care of a newborn close to death (37.7%) and/or a newborn with unfavorable neurological prognosis (30.8%). The higher the physicians’ support towards the value of quality of human life, the more probable it was that they had taken a decision to withhold or withdraw neonatal intensive care (p < 0.05). Conclusions: Our research shows that Greek NICU physicians report considerably lower levels of ethical decision-making regarding preterm and ill newborns compared to their counterparts in other European countries. Clinical practices and attitudes towards ethical decision-making appear to be influenced mainly by the Greek physicians’ values.
Understanding the Binding Induced Folding of Intrinsically Disordered Proteins by Protein Engineering: Caveats and Pitfalls
Authors: Malagrinò Francesca , Visconti Lorenzo , Pagano Livia , Toto Angelo , Troilo Francesca , Gianni Stefano
Published: May 2020

Many proteins lack a well-defined three-dimensional structure in isolation. These proteins, typically denoted as intrinsically disordered proteins (IDPs), may display a characteristic disorder-to-order transition when binding their physiological partner(s). From an experimental perspective, it is of great importance to establish the general grounds to understand how such folding processes may be explored. Here we discuss the caveats and the pitfalls arising when applying to IDPs one of the key techniques to characterize the folding of globular proteins, the Φ value analysis. This method is based on measurements of the free energy changes of transition and native states upon conservative, non-disrupting, mutations. On the basis of available data, we reinforce the validity of Φ value analysis in the study of IDPs and suggest future experiments to further validate this powerful experimental method.
MiMIF-2 Effector of Meloidogyne incognita Exhibited Enzyme Activities and Potential Roles in Plant Salicylic Acid Synthesis
Authors: Zhao Jianlong , Mao Zhenchuan , Sun Qinghua , Liu Qian , Jian Heng , Xie Bingyan
Published: May 2020

Plant-parasitic nematodes secrete a series of effectors to promote parasitism by modulating host immunity, but the detailed molecular mechanism is ambiguous. Animal parasites secrete macrophage migration inhibitory factor (MIF)-like proteins for evasion of host immune systems, in which their biochemical activities play essential roles. Previous research demonstrated that MiMIF-2 effector was secreted by Meloidogyne incognita and modulated host immunity by interacting with annexins. In this study, we show that MiMIF-2 had tautomerase activity and protected nematodes against H2O2 damage. MiMIF-2 expression not only decreased the amount of H2O2 generation during nematode infection in Arabidopsis, but also suppressed Bax-induced cell death by inhibiting reactive oxygen species burst in Nicotiana benthamiana. Further, RNA-seq transcriptome analysis and RT-qPCR showed that the expression of some heat-shock proteins was down regulated in MiMIF-2 transgenic Arabidopsis. After treatment with flg22, RNA-seq transcriptome analysis indicated that the differentially expressed genes in MiMIF-2 expressing Arabidopsis were pointed to plant hormone signal transduction, compound metabolism and plant defense. RT-qPCR and metabolomic results confirmed that salicylic acid (SA) related marker genes and SA content were significantly decreased. Our results provide a comprehensive understanding of how MiMIF-2 modulates plant immunity and broaden knowledge of the intricate relationship between M. incognita and host plants.
Small RNA Sequencing Analysis of miRNA Expression Reveals Novel Insihts into Root Formation under Root Restriction Cultivation in Grapevine (Vitis vinifera L.)
Authors: Li Hui , Gao Zhen , Zahid Muhammad Salman , Li Dongmei , Javed Hafiz Umer , Wang Lei , Song Shiren , Zhao Liping , Xu Wenping , Zhang Caixi , Ma Chao , Wang Shiping
Published: May 2020

Root restriction cultivation (RRC) can influence plant root architecture, but its root phenotypic changes and molecular mechanisms are still unknown. In this study, phenotype observations of grapevine root under RRC and control cultivation (nRC) at 12 time points were conducted, and the root phenotype showed an increase of adventitious and lateral root numbers and root tip degeneration after RRC cultivation from 70 days after planting (DAP). The 70 and 125 DAP sampling of two different cultivations, named nR70, RR70, nR125, and RR125, were selected for small RNA sequencing. A total of 153 known miRNAs and 119 predicted novel miRNAs were obtained. Furthermore, BLAST was used to predict the novel miRNAs with miRBase databases using the default parameters; 96 of the 119 predicted novel miRNAs were similar to other species, and the remaining 23 grapevine-specific novel miRNAs were obtained. There were 26, 33, 26, and 32 miRNAs that were differentially expressed in different comparison groups (RR70 vs. nR70, RR125 vs. nR125, nR125 vs. nR70 and RR125 vs. RR70). Target genes prediction of differentially expressed miRNAs was annotated on a variety of biological processes, and 24 participated in root development. Moreover, multiple miRNAs were found to jointly regulate lateral root development under root restriction conditions. The miRNA expression pattern comparison between RRC and nRC may provide a framework for the future analysis of miRNAs associated with root development in grapevine.
Functional Block of Interleukin-6 Reduces a Bone Pain Marker But Not Bone Loss in Hindlimb-Unloaded Mice
Authors: Wakabayashi Hiroki , Miyamura Gaku , Nagao Nobuto , Kato Sho , Naito Yohei , Sudo Akihiro
Published: May 2020

Interleukin-6 (IL-6) is widely accepted to stimulate osteoclasts. Our aim in this study was to examine whether the inhibitory effect of IL-6 on bone loss and skeletal pain associated with osteoporosis in hindlimb-unloaded (HU) mice in comparison with bisphosphonate. Eight-week-old male ddY mice were tail suspended for 2 weeks. Starting immediately after reload, vehicle (HU group), alendronate (HU-ALN group), or anti-IL-6 receptor antibody (HU-IL-6i group) was injected subcutaneously. After a 2-week treatment, pain-related behavior was examined using von Frey filaments. The bilateral distal femoral and proximal tibial metaphyses were analyzed three-dimensionally with micro-computed tomography. Calcitonin gene-related peptide (CGRP) expressions in dorsal root ganglion (DRG) neurons innervating the hindlimbs were examined using immunohistochemistry. HU mice with tail suspension developed bone loss. The HU mice showed mechanical hyperalgesia in the hindlimbs and increased CGRP immunoreactive neurons in the L3-5 DRG. Treatment with IL-6i and ALN prevented HU-induced mechanical hyperalgesia and upregulation of CGRP expressions in DRG neurons. Furthermore, ALN but not IL-6i prevented HU-induced bone loss. In summary, treatment with IL-6i prevented mechanical hyperalgesia in hindlimbs and suppressed CGRP expressions in DRG neurons of osteoporotic models. The novelty of this research suggests that IL-6 is one of the causes of immobility-induced osteoporotic pain regardless improvement of bone loss.
The Effects of Perceived Quality of Augmented Reality in Mobile Commerce—An Application of the Information Systems Success Model
Authors: Yoo Jungmin
Published: May 2020

Augmented reality (AR) enables consumers to browse and try products virtually by providing additional information and functionality to mobile shopping. Retailers continue to develop AR technology to engage consumers and enhance their digital shopping experiences. However, despite the growing interest in this technology, consumers rarely rely on AR due to the quality of its content. This study applies an information systems success model to examine the antecedents that influence the adoption of mobile technology, specifically focusing on consumers’ perception of AR quality and its effect on perceived diagnosticity and consumer satisfaction when using AR technology. Moreover, the study examines how perceived diagnosticity and satisfaction influence loyalty. The study participants were 283 shoppers in Korea who have previously experienced mobile shopping, with data collected through an online survey. The results show that when using AR, (1) the consumer’s perceptions of information quality and visual quality positively influence perceived diagnosticity and satisfaction, (2) perceived diagnosticity positively influences satisfaction and (3) satisfaction positively influences loyalty. These results have practical implications for mobile retailers seeking to develop effective product presentation strategies using innovative technologies.
Habitat and Seasonality Affect Mosquito Community Composition in the West Region of Cameroon
Authors: Mayi Marie Paul Audrey , Bamou Roland , Djiappi-Tchamen Borel , Fontaine Albin , Jeffries Claire L. , Walker Thomas , Antonio-Nkondjio Christophe , Cornel Anthony John , Tchuinkam Timoléon
Published: May 2020

To identify potential sylvatic, urban and bridge-vectors that can be involved in current or future virus spillover from wild to more urbanised areas, entomological field surveys were conducted in rural, peri-urban and urban areas spanning the rainy and dry seasons in western Cameroon. A total of 2650 mosquitoes belonging to 37 species and eight genera were collected. Mosquito species richness was significantly influenced by the specific combination of the habitat type and the season. The highest species richness was found in the peri-urban area (S = 30, Chao1 = 121 ± 50.63, ACE = 51.97 ± 3.88) during the dry season (S = 28, Chao1 = 64 ± 25.7, ACE = 38.33 ± 3.1). Aedes (Ae.) africanus and Culex (Cx.) moucheti were only found in the rural and peri-urban areas, while Cx. pipiens s.l. and Ae. aegypti were only found in the urban area. Cx. (Culiciomyia) spp., Cx. duttoni and Ae. albopictus were caught in the three habitat types. Importantly, approximately 52% of the mosquito species collected in this study have been implicated in the transmission of diverse arboviruses. This entomological survey provides a catalogue of the different mosquito species that may be involved in the transmission of arboviruses. Further investigations are needed to study the vectorial capacity of each mosquito species in arbovirus transmission.
A Historical Review of Management Options Used against the Stable Fly (Diptera: Muscidae)
Authors: Cook David
Published: May 2020

The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), remains a significant economic pest globally in situations where intensive animal production or horticultural production provide a suitable developmental medium. Stable flies have been recorded as pests of livestock and humans since the late 1800s to early 1900s. Over 100 years of research has seen numerous methodologies used to control this fly, in particular to protect cattle from flies to minimise production losses. Reduced milk production in dairy cows and decreased weight gain in beef cattle account for losses in the US alone of > $2000 million annually. Rural lifestyles and recreation are also seriously affected. Progress has been made on many control strategies against stable fly over a range of chemical, biological, physical and cultural options. This paper reviews management options from both a historical and a technical perspective for controlling this pest. These include the use of different classes of insecticides applied to affected animals as toxicants or repellents (livestock and humans), as well as to substrates where stable fly larvae develop. Arthropod predators of stable flies are listed, from which potential biological control agents (e.g., wasps, mites, and beetles) are identified. Biopesticides (e.g., fungi, bacteria and plant-derived products) are also discussed along with Integrated Pest Management (IPM) against stable flies for several animal industries. A review of cultural and physical management options including trapping, trap types and methodologies, farm hygiene, scheduled sanitation, physical barriers to fly emergence, livestock protection and amendments added to animal manures and bedding are covered. This paper presents a comprehensive review of all management options used against stable flies from both a historical and a technical perspective for use by any entomologist, livestock producer or horticulturalist with an interest in reducing the negative impact of this pest fly.
The Evolving Role of Natural Compounds in the Medical Treatment of Uterine Fibroids
Authors: Ciebiera Michał , Ali Mohamed , Prince Lillian , Jackson-Bey Tia , Atabiekov Ihor , Zgliczyński Stanisław , Al-Hendy Ayman
Published: May 2020

Uterine fibroids (UFs) remain a significant health issue for many women, with a disproportionate impact on women of color, likely due to both genetic and environmental factors. The prevalence of UFs is estimated to be approximately 70% depending on population. UF-derived clinical symptoms include pelvic pain, excessive uterine bleeding, gastrointestinal and voiding problems, as well as impaired fertility. Nowadays numerous methods of UF treatment are available—from conservative treatment to invasive surgeries. Selecting an appropriate treatment option should be individualized and adjusted to the patient's expectations as much as possible. So far, the mainstay of treatment is surgery, but their negative impact of future fertility is clear. On the other hand, emerging new pharmaceutical options have significant adverse effects like liver function impairment, hot flashes, bone density loss, endometrial changes, and inability to attempt conception during treatment. Several natural compounds are found to help treat UFs and relieve their symptoms. In this review we summarize all the current available data about natural compounds that may be beneficial for patients with UFs, especially those who want to preserve their future fertility or have treatment while actively pursuing conception. Vitamin D, epigallocatechin gallate, berberine, curcumin, and others are being used as alternative UF treatments. Moreover, we propose the concept of using combined therapies of natural compounds on their own or combined with hormonal agents to manage UFs. There is a strong need for more human clinical trials involving these compounds before promoting widespread usage.
Involvement of Autonomic Nervous System in New-Onset Atrial Fibrillation during Acute Myocardial Infarction
Authors: Sagnard Audrey , Guenancia Charles , Mouhat Basile , Maza Maud , Fichot Marie , Moreau Daniel , Garnier Fabien , Lorgis Luc , Cottin Yves , Zeller Marianne
Published: May 2020

Background: Atrial fibrillation (AF) is common after acute myocardial infarction (AMI) and associated with in-hospital and long-term mortality. However, the pathophysiology of AF in AMI is poorly understood. Heart rate variability (HRV), measured by Holter-ECG, reflects cardiovascular response to the autonomic nervous system and altered (reduced or enhanced) HRV may have a major role in the onset of AF in AMI patients. Objective: We investigated the relationship between autonomic dysregulation and new-onset AF during AMI. Methods: As part of the RICO survey, all consecutive patients hospitalized for AMI at Dijon (France) university hospital between June 2001 and November 2014 were analyzed by Holter-ECG <24 h following admission. HRV was measured using temporal and spectral analysis. Results: Among the 2040 included patients, 168 (8.2%) developed AF during AMI. Compared to the sinus-rhythm (SR) group, AF patients were older, had more frequent hypertension and lower left ventricular ejection fraction LVEF. On the Holter parameters, AF patients had higher pNN50 values (11% vs. 4%, p < 0.001) and median LH/HF ratio, a reflection of sympathovagal balance, was significantly lower in the AF group (0.88 vs 2.75 p < 0.001). The optimal LF/HF cut-off for AF prediction was 1.735. In multivariate analyses, low LF/HF <1.735 (OR(95%CI) = 3.377 (2.047–5.572)) was strongly associated with AF, ahead of age (OR(95%CI) = 1.04(1.01–1.06)), mean sinus-rhythm rate (OR(95%CI) = 1.03(1.02–1.05)) and log NT-proBNP (OR(95%CI) = 1.38(1.01–1.90). Conclusion: Our study strongly suggests that new-onset AF in AMI mainly occurs in a dysregulated autonomic nervous system, as suggested by low LF/HF, and higher PNN50 and RMSSD values.
Clinical, Radiological, and Laboratory Features of Spinal Cord Involvement in Primary Sjögren’s Syndrome
Authors: Butryn Michaela , Neumann Jens , Rolfes Leoni , Bartels Claudius , Wattjes Mike P. , Mahmoudi Nima , Seeliger Tabea , Konen Franz F. , Thiele Thea , Witte Torsten , Meuth Sven G. , Skripuletz Thomas , Pawlitzki Marc
Published: May 2020

Objective: To identify radiological and laboratory hallmarks in patients with primary Sjögren’s syndrome (pSS) presenting with spinal cord involvement. Methods: Clinical and laboratory routine parameters were analyzed in a retrospective multicenter case series of four patients who developed myelitis associated with pSS. Serological and cerebrospinal fluid (CSF) measurements of pSS associated anti-SSA(Ro)-antibodies were initiated, and CSF neurofilament light chain (NFL) levels were assessed. NFL values were compared with results from 15 sex- and age-matched healthy controls. Radiological assessment was performed using multi-sequence spinal cord magnetic resonance imaging. Results: Three of the four patients initially developed neurological signs suggestive of myelitis and were subsequently diagnosed with pSS. All patients presented a longitudinal spinal T2-hyperintense lesion in the cervical spinal cord, whereas only two patients showed pleocytosis and oligoclonal bands in the CSF. Median (range) CSF-NFL levels were significantly elevated in patients compared to controls (6672 pg/mL (621–50,000) vs. 585 pg/mL (357–729), p = 0.009). One patient showed sustained, highly increased NFL levels (50,000 pg/mL) in the initial assessment when radiological signs of axonal injury were still absent. Anti-SSA(Ro)-antibodies were found in the serum of three patients, while two patients additionally presented intrathecal anti-SSA(Ro)-antibody production. Elevated CSF-NFL levels and intrathecal synthesis of anti-SSA(Ro)-antibodies were associated with a relapsing and treatment-resistant disease course. Conclusion: Inflammatory spinal cord lesions associated with pSS are a rare but serious disease leading to severe disability. NFL and anti-SSA(Ro)-antibodies in CSF might serve as prognostic biomarkers and should be routinely assessed in patients with pSS.
Asthma/Rhinitis (The United Airway) and Allergy: Chicken or Egg; Which Comes First?
Authors: Warner John O.
Published: May 2020

While allergy, asthma and rhinitis do not inevitably co-exist, there are strong associations. Not all those with asthma are allergic, rhinitis may exist without asthma, and allergy commonly exists in the absence of asthma and/or rhinitis. This is likely due to the separate gene/environment interactions which influence susceptibility to allergic sensitization and allergic airway diseases. Allergic sensitization, particularly to foods, and eczema commonly manifest early in infancy, and not infrequently are followed by the development of allergic rhinitis and ultimately asthma. This has become known as the “allergic march”. However, many infants with eczema never develop asthma or rhinitis, and both the latter conditions can evolve without prior eczema or food allergy. Understanding the mechanisms underlying the ontogeny of allergic sensitization and allergic disease will facilitate rational approaches to the prevention and management of asthma and allergic rhinitis. Furthermore, a range of new, so-called biological, therapeutic approaches, targeting specific allergy-promoting and pro-inflammatory molecules, are now in clinical trials or have been recently approved for use by regulatory authorities and could have a major impact on disease prevention and control in the future. Understanding basic mechanisms will be essential to the employment of such medications. This review will explain the concept of the united airway (rhinitis/asthma) and associations with allergy. It will incorporate understanding of the role of genes and environment in relation to the distinct but interacting origins of allergy and rhinitis/asthma. Understanding the patho-physiological differences and varying therapeutic requirements in patients with asthma, with or without rhinitis, and with or without associated allergy, will aid the planning of a personalized evidence-based management strategy.
Clinician Assessment Tools for Patients with Diabetic Foot Disease: A Systematic Review
Authors: Fernández-Torres Raúl , Ruiz-Muñoz María , Pérez-Panero Alberto J. , García-Romero Jerónimo C. , Gónzalez-Sánchez Manuel
Published: May 2020

The amputation rate in patients with diabetes is 15 to 40 times higher than in patients without diabetes. To avoid major complications, the identification of high-risk in patients with diabetes through early assessment highlights as a crucial action. Clinician assessment tools are scales in which clinical examiners are specifically trained to make a correct judgment based on patient outcomes that helps to identify at-risk patients and monitor the intervention. The aim of this study is to carry out a systematic review of valid and reliable Clinician assessment tools for measuring diabetic foot disease-related variables and analysing their psychometric properties. The databases used were PubMed, Scopus, SciELO, CINAHL, Cochrane, PEDro, and EMBASE. The search terms used were foot, ankle, diabetes, diabetic foot, assessment, tools, instruments, score, scale, validity, and reliability. The results showed 29 validated studies with 39 Clinician assessment tools and six variables. There is limited evidence on all of the psychometric characteristics of the Clinician assessment tools included in this review, although some instruments have been shown to be valid and reliable for the assessment of diabetic neuropathy (Utah Early Neuropathy Scale or UENS); ulceration risk (Queensland High Risk Foot Form or QHRFF); diabetic foot ulcer assessment, scoring, and amputation risk (Perfusion, extent, depth, infection and sensation scale or PEDIS and Site, Ischemia, Neuropathy, Bacterial Infection, and Depth score or SINBAD); and diabetic foot ulcer measurement (Leg Ulcer Measurement Tool LUMT).
Forecasting COVID-19-Associated Hospitalizations under Different Levels of Social Distancing in Lombardy and Emilia-Romagna, Northern Italy: Results from an Extended SEIR Compartmental Model
Authors: Reno Chiara , Lenzi Jacopo , Navarra Antonio , Barelli Eleonora , Gori Davide , Lanza Alessandro , Valentini Riccardo , Tang Biao , Fantini Maria Pia
Published: May 2020

The outbreak of coronavirus disease 2019 (COVID-19) was identified in Wuhan, China, in December 2019. As of 17 April 2020, more than 2 million cases of COVID-19 have been reported worldwide. Northern Italy is one of the world’s centers of active coronavirus cases. In this study, we predicted the spread of COVID-19 and its burden on hospital care under different conditions of social distancing in Lombardy and Emilia-Romagna, the two regions of Italy most affected by the epidemic. To do this, we used a Susceptible-Exposed-Infectious-Recovered (SEIR) deterministic model, which encompasses compartments relevant to public health interventions such as quarantine. A new compartment L was added to the model for isolated infected population, i.e., individuals tested positives that do not need hospital care. We found that in Lombardy restrictive containment measures should be prolonged at least until early July to avoid a resurgence of hospitalizations; on the other hand, in Emilia-Romagna the number of hospitalized cases could be kept under a reasonable amount with a higher contact rate. Our results suggest that territory-specific forecasts under different scenarios are crucial to enhance or take new containment measures during the epidemic.
From City- to Site-Dimension: Assessing the Urban Ecosystem Services of Different Types of Green Infrastructure
Authors: Brzoska Patrycia , Spāģe Aiga
Published: May 2020

Cities have a wide variety of green infrastructure types, such as parks and gardens. These structures can provide important ecosystem services (ES) with a major impact on human well-being. With respect to urban planning, special consideration must be given to such green infrastructure types when implementing measures to maintain and enhance the quality of life. Therefore, generating knowledge on the urban ES of differently scaled green infrastructure types is important. This systematic literature review provides an overview of existing studies which have explicitly investigated the urban ES of differently spatial-scaled green infrastructure types. By reviewing 76 publications, we confirm rising academic interest in this topic. The most frequently assessed urban ES belong to the category Regulating and Maintenance. Only a few have considered individual small structures such as green roofs or single gardens; green spaces are often aggregated into one, mostly city-wide, object of investigation, with resulting oversimplifications. Moreover, generalizing methods are mostly applied. Simultaneously, many studies have applied methods to evaluate location-specific primary data. More research is needed on small-scale structures, in particular to consider site-, and thus location-specific, parameters in order to successfully implement the ES concept into urban planning and to obtain realistic results for ES assessments.
Effects of Metacognitive Strategy Training on Chinese Listening Comprehension
Authors: Liu Yanmei
Published: May 2020

In an exploration of solutions to improve Chinese second language learners’ listening comprehension, this quantitative quasi-experimental study examines the effects of metacognitive strategy training (MST) on learners’ metacognitive awareness, listening performance, and proficiency in an intensive language training program. In contrast with the extant research, this study designed a metacognitive learning cycle model, including self-diagnosis, planning, monitoring, evaluation, regulation, and reflection strategies, as the content of the MST. Six classes, comprised of a total of 80 participants, were assigned into three groups: Self-directed, teacher-led, and control groups. The Metacognitive Awareness Listening Questionnaire and a listening comprehension test were administered as pre- and post-tests, in addition to a proficiency test as a post-test only. Results demonstrate no significant differences in metacognitive awareness development, listening performance gains, and proficiency test results among the three groups. The results do indicate that the self-directed MST better enhances development of students’ planning and evaluation awareness, and teacher-led MST workshops with special emphasis on the area of monitoring strategy will help students raise awareness. The findings of this study reveal that insufficient training time and MST without the integration of cognitive strategies do not yield significant effects. It is suggested that future MSTs should involve sufficient training time and effective follow-ups to ensure its positive effects. This study proposes that the effectiveness of MST could be improved by combining it with cognitive strategies training.
Global Stability Analysis of Fractional-Order Quaternion-Valued Bidirectional Associative Memory Neural Networks
Authors: Humphries Usa , Rajchakit Grienggrai , Kaewmesri Pramet , Chanthorn Pharunyou , Sriraman Ramalingam , Samidurai Rajendran , Lim Chee Peng
Published: May 2020

We study the global asymptotic stability problem with respect to the fractional-order quaternion-valued bidirectional associative memory neural network (FQVBAMNN) models in this paper. Whether the real and imaginary parts of quaternion-valued activation functions are expressed implicitly or explicitly, they are considered to meet the global Lipschitz condition in the quaternion field. New sufficient conditions are derived by applying the principle of homeomorphism, Lyapunov fractional-order method and linear matrix inequality (LMI) approach for the two cases of activation functions. The results confirm the existence, uniqueness and global asymptotic stability of the system’s equilibrium point. Finally, two numerical examples with their simulation results are provided to show the effectiveness of the obtained results.
Predictive Power of Adaptive Candlestick Patterns in Forex Market. Eurusd Case
Authors: Orquín-Serrano Ismael
Published: May 2020

The Efficient Market Hypothesis (EMH) states that all available information is immediately reflected in the price of any asset or financial instrument, so that it is impossible to predict its future values, making it follow a pure stochastic process. Among all financial markets, FOREX is usually addressed as one of the most efficient. This paper tests the efficiency of the EURUSD pair taking only into consideration the price itself. A novel categorical classification, based on adaptive criteria, of all possible single candlestick patterns is presented. The predictive power of candlestick patterns is evaluated from a statistical inference approach, where the mean of the average returns of the strategies in out-of-sample historical data is taken as sample statistic. No net positive average returns are found in any case after taking into account transaction costs. More complex candlestick patterns are considered feeding supervised learning systems with the information of past bars. No edge is found even in the case of considering the information of up to 24 preceding candlesticks.
Finite Element Analysis of Ball Burnishing on Ball-End Milled Surfaces Considering Their Original Topology and Residual Stress
Authors: Amini Cyrus , Jerez-Mesa Ramón , Travieso-Rodriguez J. Antonio , Llumà Jordi , Estevez-Urra Aida
Published: May 2020

Ball burnishing is a superfinishing operation whose objective is the enhancement of surface integrity of previously machined surfaces, hence its appropriateness to complement chip removal processes at the end of a production line. As a complex process involving plastic deformation, friction and three-dimensional interaction between solids, numerical solutions and finite element models have typically included a considerable amount of simplifications that represent the process partially. The aim of this paper is to develop a 3D numerical finite element model of the ball burnishing process including in the target workpiece real surface integrity descriptors resulting from a ball-end milled AISI 1038 surface. Specifically, its periodical topological features are used to generate the surface geometry and the residual stress tensor measured on a real workpiece is embedded in the target surface. Secondly, different models varying the effect of the coefficient of friction and the direction of application of burnishing passes with regards to the original milling direction are calculated. Results show that the resulting topology and residual stresses are independent of the burnishing direction. However, it is evident that the model outputs are highly influenced by the value of the coefficient of friction. A value of 0.15 should be implemented in order to obtain representative results through finite element models.
Reaction Mechanism and Process Control of Hydrogen Reduction of Ammonium Perrhenate
Authors: Tang Junjie , Sun Yuan , Zhang Chunwei , Wang Long , Zhou Yizhou , Fang Dawei , Liu Yan
Published: May 2020

The preparation of rhenium powder by a hydrogen reduction of ammonium perrhenate is the only industrial production method. However, due to the uneven particle size distribution and large particle size of rhenium powder, it is difficult to prepare high-density rhenium ingot. Moreover, the existing process requires a secondary high-temperature reduction and the deoxidization process is complex and requires a high-temperature resistance of the equipment. Attempting to tackle the difficulties, this paper described a novel process to improve the particle size distribution uniformity and reduce the particle size of rhenium powder, aiming to produce a high-density rhenium ingot, and ammonium perrhenate is completely reduced by hydrogen at a low temperature. When the particle size of the rhenium powder was 19.74 µm, the density of the pressed rhenium ingot was 20.106 g/cm3, which was close to the theoretical density of rhenium. In addition, the hydrogen reduction mechanism of ammonium perrhenate was investigated in this paper. The results showed that the disproportionation of ReO3 decreased the rate of the reduction reaction, and the XRD and XPS patterns showed that the increase in the reduction temperature was conducive to increasing the reduction reaction rate and reducing the influence of disproportionation on the reduction process. At the same reduction temperature, reducing the particle sizes of ammonium perrhenate was conducive to increasing the hydrogen reduction rate and reducing the influence of the disproportionation.
Metabolite Genome-Wide Association Study (mGWAS) and Gene-Metabolite Interaction Network Analysis Reveal Potential Biomarkers for Feed Efficiency in Pigs
Authors: Wang Xiao , Kadarmideen Haja N.
Published: May 2020

Metabolites represent the ultimate response of biological systems, so metabolomics is considered the link between genotypes and phenotypes. Feed efficiency is one of the most important phenotypes in sustainable pig production and is the main breeding goal trait. We utilized metabolic and genomic datasets from a total of 108 pigs from our own previously published studies that involved 59 Duroc and 49 Landrace pigs with data on feed efficiency (residual feed intake (RFI)), genotype (PorcineSNP80 BeadChip) data, and metabolomic data (45 final metabolite datasets derived from LC-MS system). Utilizing these datasets, our main aim was to identify genetic variants (single-nucleotide polymorphisms (SNPs)) that affect 45 different metabolite concentrations in plasma collected at the start and end of the performance testing of pigs categorized as high or low in their feed efficiency (based on RFI values). Genome-wide significant genetic variants could be then used as potential genetic or biomarkers in breeding programs for feed efficiency. The other objective was to reveal the biochemical mechanisms underlying genetic variation for pigs’ feed efficiency. In order to achieve these objectives, we firstly conducted a metabolite genome-wide association study (mGWAS) based on mixed linear models and found 152 genome-wide significant SNPs (p-value < 1.06 × 10−6) in association with 17 metabolites that included 90 significant SNPs annotated to 52 genes. On chromosome one alone, 51 significant SNPs associated with isovalerylcarnitine and propionylcarnitine were found to be in strong linkage disequilibrium (LD). SNPs in strong LD annotated to FBXL4, and CCNC consisted of two haplotype blocks where three SNPs (ALGA0004000, ALGA0004041, and ALGA0004042) were in the intron regions of FBXL4 and CCNC. The interaction network revealed that CCNC and FBXL4 were linked by the hub gene N6AMT1 that was associated with isovalerylcarnitine and propionylcarnitine. Moreover, three metabolites (i.e., isovalerylcarnitine, propionylcarnitine, and pyruvic acid) were clustered in one group based on the low-high RFI pigs. This study performed a comprehensive metabolite-based genome-wide association study (GWAS) analysis for pigs with differences in feed efficiency and provided significant metabolites for which there is significant genetic variation as well as biological interaction networks. The identified metabolite genetic variants, genes, and networks in high versus low feed efficient pigs could be considered as potential genetic or biomarkers for feed efficiency.