bluuebunny/crossref_metadata_2025 huggingface dataset:
Emprendimiento Social: Facilitadores personales percibidos por estudiantes universitarios durante la pandemia en Argentina
Authors: Silva-Peralta Yamila Fernanda , Rompato María Emilia , Pujol-Cols Lucas
Published: February 2023

La mayoría de las investigaciones sobre la emergencia socio-sanitaria causada por la pandemia del COVID-19 se han focalizado en sus efectos negativos. Este artículo tiene por objetivo explorar los factores personales que facilitan el emprendimiento social en este contexto en Argentina. A través de un enfoque de teoría fundada, este estudio analiza los discursos de una muestra de estudiantes avanzados de una universidad pública argentina. Los hallazgos revelan que los estudiantes con ciertas características personales, como creencias más positivas acerca de la transformación social, una orientación prosocial y competencias emprendedoras, asumen un rol importante frente a la emergencia socio-sanitaria a través del emprendimiento social.
Impulse oscillometry bronchodilator response in preschool children
Authors: Schulze Johannes , Meoli A. , Trischler Jordis , Hutter M. , Dressler Melanie , Esposito Susanna , Blümchen K. , Zielen Stefan
Published: February 2023

Background: In preschoolers, performing an acceptable
spirometry and measuring bronchodilator response (BDR) is challenging;
impulse oscillometry (IOS) may be an alternative to spirometry. However,
there is still no consensus in standardization of BDR for IOS in young
children. Objective: The objective of the study was to identify
optimal thresholds to define a positive BDR test with IOS.
Methods: Young infants aged 3 to 6 years with suspected asthma
were evaluated in a real life setting with both IOS and spirometry pre-
and post-BDR. The BDR was defined as positive when the change of FEV1
was ≥12% and/or ≥200 mL. Results: Among 72 patients (age 4.98
± 0.94 years; 64% boys), 36 (age 5.15 ± 0.99 years; 64% boys) were
selected for the subsequent analysis according to ATS / ERS quality
criteria of measurements. The spirometric BDR was found positive in
seven subjects (19.4%). In IOS, the mean decrease in R5 and AX was
19.86% ± 10.04 and 44% ± 22.10, and the mean increase in X5 was
23.28% ± 17.82, respectively. A decrease in R5 of 25.7% (AUC 0.77, p =
0.03) and an increase in X5 of 25.7% (AUC 0.75, p = 0.04) showed the
best combination of sensitivity and specificity to detect an increase of
FEV1 ≥12% and/or ≥200 mL. Conclusion: The IOS may present a
valid alternative to spirometry to measure BDR in preschool children. We
are considering a decrease of 26% in R5 and an increase of 26% in X5
as diagnostic threshold for BDR.
Towards The Ultimate Brain: Exploring Scientific Discovery with ChatGPT AI
Authors: Adesso Gerardo
Published: February 2023

This paper presents a novel approach to scientific discovery using an
artificial intelligence (AI) environment known as ChatGPT, developed by
OpenAI. This is the first paper entirely generated with outputs from
ChatGPT. We demonstrate how ChatGPT can be instructed through a
gamification environment to define and benchmark hypothetical physical
theories. Through this environment, ChatGPT successfully simulates the
creation of a new improved model, called GPT , which
combines the concepts of GPT in AI (generative pretrained transformer)
and GPT in physics (generalized probabilistic theory). We show that
GPT can use its built-in mathematical and statistical
capabilities to simulate and analyze physical laws and phenomena. As a
demonstration of its language capabilities, GPT also
generates a limerick about itself. Overall, our results demonstrate the
promising potential for human-AI collaboration in scientific discovery,
as well as the importance of designing systems that effectively
integrate AI’s capabilities with human intelligence.
Fundraising scenarios for state-founded universities
Authors:   Latvia University of Life Sciences and Technologies, Kundzina Laila , Rivza Baiba ,   Latvia University of Life Sciences and Technologies, Rivza Peteris ,   Latvia University of Life Sciences and Technologies
Published: February 2023

The creation of fundraising scenarios for state-founded universities and the selection of the most appropriate scenario is an important factor in the successful attraction of donations. The study will provide an analysis of three possible scenarios for fundraising, which have been evaluated by seven experts: representatives of patrons as private persons and enterprises, patron's fellow, a representative of the municipality, a representative of the Ministry of Education and Science, a representative of a higher education institution and a representative of a foundation. The first scenario consists of activities aimed at maintaining the existing fundraising trends. The second scenario is associated with a greater involvement of the municipality in attracting patrons to universities, whereas the third scenario comprises a greater state involvement in attracting patrons to universities. Expert opinions were processed using the Analytic Hierarchy Process (AHP) method. Choosing the best-case scenario will create an opportunity for philanthropic organizations of 12 state-founded universities to cooperate more fully with patrons in fundraising for their universities, projects and students.
Labour market characteristics of persons with disability in Latvia
Authors:   Latvia University of Life Sciences and Technologies, Licite-Kurbe Lasma , Sevcuna Liva ,   Latvia University of Life Sciences and Technologies
Published: February 2023

The goal of the Union of Equality: Strategy for the Rights of Persons with Disabilities 2021-2030 is to ensure that persons with disabilities in Europe have equal opportunities, including equal access to participate in society and the economy. In 2020, 201 598 persons with disabilities lived in Latvia (10.6% of the total population), moreover, their number is growing every year. Only 26.6% people with disabilities are employed, and their employment is slightly growing every year. Besides, unemployment increases the risk of falling below the poverty line, as well as increases dependence on municipal and national social benefits. The aim of the present research is to analyse the situation of persons with disabilities in the labour market and identify the factors influencing their employment in Latvia. The monographic, analysis and synthesis methods, as well as statistical analysis methods were used to achieve the aim. The research has concluded that the employment of people with disabilities is affected by the support instruments embedded in the legislation and specific support instruments for work integration social enterprises. Overall, however, the support instruments for the integration of people with disabilities into the labour market are few or insufficiently encourage entrepreneurs. To increase the employment of persons with disabilities, it is important to promote the development of social entrepreneurship, in particular the development of work integration social enterprises. Keywords: persons with disability, work integration social enterprise, employment, labour market.
Society in the shackles of surveillance capitalism
Authors:   University of Innsbruck, Metra Ritvars , Dobelniece Signe ,   Latvia University of Life Sciences and Technologies
Published: February 2023

The rapid development of information and communications technologies has changed the lives of both - society as a whole and individuals in it. The world is becoming a huge system for gathering, storing, and sharing information. Large amounts of data are collected, stored, analysed, and used for commercial purposes. Prominent American sociologist Shoshana Zuboff calls this process "surveillance capitalism." This paper aims to analyse the tools of surveillance capitalism and the influence of surveillance capitalism on public choices and behaviour. It is based on empirical information collected using several research methods - a survey (n = 204), semi-structured interviews (n = 5), and qualitative content analysis. The paper analyses three popular surveillance capitalists' online tools - Google search, Facebook, and Twitter and gives insights into society's preferences and behaviour patterns concerning ways of obtaining information and attitudes towards privacy and self-censorship. The research concludes that the tools created by surveillance capitalists have become an integral part of social life, and they dominate the process of interaction. They make life easier but their uncritical acceptance can be dangerous. Since surveillance capitalism has taken the stage, society members often uncritically, even ignorantly, treat current reality in which the everydayness data about user's life are the free raw material for surveillance capitalists, thus contributing to surveillance capitalism and its threats to defined privacy principles and democracy.
Performance of the Dexcom G6 Continuous Glucose Monitoring System During Cardiac Surgery Using Hypothermic Extracorporeal Circulation
Authors: Herzig David , Vettoretti Martina , Guensch Dominik P. , Melmer Andreas , Schürch Daniel , Roos Jonathan , Goerg Arna M.C. , Krutkyte Gabija , Cecchini Luca , Facchinetti Andrea , Vogt Andreas P. , Bally Lia
Published: February 2023

OBJECTIVE

Continuous glucose monitoring (CGM) may be challenged by extreme conditions during cardiac surgery using hypothermic extracorporeal circulation (ECC).

RESEARCH DESIGN AND METHODS

We evaluated the Dexcom G6 sensor in 16 subjects undergoing cardiac surgery with hypothermic ECC, of whom 11 received deep hypothermic circulatory arrest (DHCA). Arterial blood glucose, quantified by the Accu-Chek Inform II meter, served as reference.

RESULTS

Intrasurgery mean absolute relative difference (MARD) of 256 paired CGM/reference values was 23.8%. MARD was 29.1% during ECC (154 pairs) and 41.6% immediately after DHCA (10 pairs), with a negative bias (signed relative difference: −13.7%, −26.6%, and −41.6%). During surgery, 86.3% pairs were in Clarke error grid zones A or B and 41.0% of sensor readings fulfilled the International Organization for Standardization (ISO) 15197:2013 norm. Postsurgery, MARD was 15.0%.

CONCLUSIONS

Cardiac surgery using hypothermic ECC challenges the accuracy of the Dexcom G6 CGM although recovery appears to occur thereafter.
Involvement of transposons in epigenetic regulation of embryogenesis
Authors: Mustafin R. N
Published: February 2023

The systems that control DNA methylation and histone modifications in embryonic development are still considered unknown, although their study is promising for the development of stem cell genetics. This review article is devoted to the description of evidence that the drivers of changes in epigenetic factors of stem cells in their successive divisions are species-specific patterns of activation of transposable elements formed in evolution. These patterns are due to the sensitivity of transposons to the influence of the microenvironment and environmental factors, as well as the functioning of their processed transcripts as noncoding RNAs. A large amount of evidence has been accumulated that many protein-coding genes originate from transposable elements, including those involved in DNA methylation and histone modification. Moreover, transposons are key sources of binding sites for transcription factors, promoters, enhancers, silencers, insulators, as well as small and long non-coding RNAs that have an epigenetic effect on gene expression at the transcriptional and post-transcriptional levels. In evolution, transposons were the sources of origin for spliceoso-mal introns and components of the spliceosome, alternative sites and regulators of splicing. The identification of specific transposons that serve as drivers of stem cells at certain stages can become the basis for their optimal control using noncoding RNAs.
Features of cytokine production in tumor associated with metastasis and basal cell hyperplasia of bronchial epithelium in non-small cell lung cancer
Authors: Pismenny D. S , Durova A. A , Krahmal' N. V , Stepanov I. V , Rodionov E. O , Eryomin V. A , Andryuhova E. S , Savelieva O. E , Zavyalova M. V , Pankova O. V , Tashireva L. A , Vtorushin S. V , Miller S. V , Tuzikov S. A , Perelmuter V. M
Published: February 2023

The emergence and progression of a tumor (relapses, metastasis) is largely due to the presence and nature of inflammatory processes. It is promising to clarify the pathogenetic role and prognostic value of cytokines involved in the inflammation. The basal cell hyperplasia in the bronchial epithelium adjacent to the tumor may be associated with inflammation in the tumor microenvironment. Objective: to study the production of inflammatory cytokines in the tumor, taking into account basal cell hyperplasia in the bronchial epithelium adjacent to the tumor in the patients with nonsmall cell lung cancer. 35 patients with non-small cell lung cancer (T1-3N0-2M0) were included in the study. Neoadjuvant chemotherapy was administered in 17 cases. The production of TGF-β, TNF-α, SDF-1, VEGFA was determined by immunohistochemistry in tumor cells and alveolar macrophages. Basal cell hyperplasia in the bronchi adjacent to the tumor was morphologically diagnosed. The absence of SDF-1 in the nuclei of tumor cells was associated with the hematogenous metastases in patients with BCH of the bronchi adjacent to the tumor. Basal cell hyperplasia is correlated with an increased TGF-β production in alveolar macrophages and a decreased of SDF-1 in the cytoplasm of tumor cells. The frequency of case TNF-α in alveolar macrophages is reduced in patients with hematogenous metastases who received neoadjuvant chemotherapy. Cytokine production in the tumor cells and leukocytes was associated with the hematogenous metastases, taking into account the basal cell hyperplasia, which is associated with the pro-inflammatory cytokines in the tumor. The effects of neoadjuvant chemotherapy differ depending hematogenous metastasis.
Cryopreservation of gametes: history and current state of a question
Authors: Odintsova I. A , Rusakova S. E , Schmidt A. A , Timoshkova Y. L
Published: February 2023

Development of reproductive medicine, namely, extracorporal fertilization, set the task of preservation and preserving of biological suitability of female and men's gametes for scientific community. Opening of a method of a vitrification showed its benefit before other methods of a cryopreservation and became the encouraging event in development of cryobank of gametes. In this work the history of emergence of a method of a cryopreservation is described; modern aspects of a cryopreservation of gametes; shortcomings and benefits of various techniques of selection of full-fledged gametes are reflected; some protocols of carrying out a cryo-preservation are provided; short characteristic of separately used cryoprotectors and their various combinations capable more effectively to protect the freezing objects from damage, than, each of cryoprotectors separately is given. It is noted that in modern literature special attention is paid to consideration of mechanisms of cryodamages and cryoprotection during the freezing and a vitrification. Characteristics of the changes happening in gametes after the procedure of freezing and thawing are discussed. Modern techniques of assessment of viability of gametes before and after a cryopreservation are given. It is shown that violation of compaction of chromatin and fragmentation of DNA in morphologically not changed spermiya has negative impact and on quality of embryos. The benefit of a structurally functional condition of oocytes of mammals at a cryopreservation of oocytes in structure is emphasized an oocyte-kumulyusnykh of complexes in comparison with the oocytes which ripened in the kultivatsionny environment.
Non-viral gene transfer in hydrogel matrices with octacalcium phosphate microgranules in optimization of reparative osteogenesis
Authors: Bozo I. Y , Presnyakov E. V , Rochev E. S , Tserceil V. V , Podluzny P. S , Mavlikeev M. O , Fedotov A. Yu , Baranov O. V , Eremin I. I , Pulin A. A , Chauzova T. S , Petrikina A. P , Bilyalov A. I , Titova A. A , Isaev A. A , Komlev V. S , Deev R. V
Published: February 2023

There is a strong unmet need for bone grafts and substitutes combining osteoinductive capacities and biophysical properties for using them in minimally invasive surgical interventions. We have developed three variants of injectable gene-activated bone substitutes containing three components: octacalcium phosphate microgranules (OCP), plasmid DNA delivering the gene of vascular endothelial growth factor, and one of the hydrogels based on sodium alginate, type I collagen, and hyaluronic acid. The molecules of the gene constructs were contained both in the hydrogel and on the surface of the OCP microgranules. In the model of a critical-sized bone defect in rabbit parietal bone, we found that all the gene-activated hydrogels contributed to bone tissue regeneration, however, the largest amount of newly formed bone, including those in the central part of the defect, was detected in the groups with gene-activated materials based on hyaluronic acid and collagen. Thus, the developed materials can be considered as candidates for medical devices, but additional studies are required to assess the dose-depended effect and optimize the materials composition.
Modern concepts about genetic regulation of connective tissue gystophysiology and its relationship to the physical quality of "flexibility”
Authors: Zholinsky A. V , Kadykova A. I , Deev R. V
Published: February 2023

"Flexibility” is a physical quality of a person, which is characterized by the ability to perform movements with a large amplitude. Flexibility is important for success in such activities as sports (artistic and rhythmic gymnastics, figure skating, etc.), as well in classical choreography, for example, ballet. Extracellular matrix producing cells and structural proteins of connective tissues take an active part in the formation of mobility of the elements of the musculoskeletal system. Connective tissues are a complex structural and functional system, the components of which are encoded by many genes. Mutations in them lead to various hereditary diseases that increase or decrease "flexibility”. The role of genes in the formation of conditions encoded in the ICD-11 LD28.Z remains unclear - "Syndromes involving connective tissue as the main feature, unspecified”, and their prognostic significance for people experiencing intense physical exertion. The purpose of this review is to generalize modern ideas about the role of genes, extracellular matrix and cells producing it in the formation of such a physical quality as flexibility.
The relationship between myeloid-derived supressor cells and clinico-laboratory parameters in patients with liver cyrrosis
Authors: Leplina O. Yu , Tikhonova M. A , Tyrinova T. V , Meledina I. V , Zheltova O. I , Ostanin A. A , Chernykh E. R
Published: February 2023

In the present study, we used multicolor flow cytometry assay to measure the numbers of various myeloid suppressor cell subpopulations (Lin-HLA-DR-CD33+, Lin-HLA-DR-CD33+CD66b+, and CD14+HLA-DRlow/-) in peripheral blood of 51 participants, including 33 patients with viral- and 1 8 patients with «nonviral» (alcoholic or biliary/autoimmune) liver cirrhosis. Patients in both groups had increased proportions of Lin-HLA-DR-CD33+, Lin-HLA-DR-CD33+CD66b+, and CD 14+HLA-DRlow/-cells which levels did not depend on the type and replication of the virus in viral liver cirrhosis. In viral liver cirrhosis, the relative numbers of Lin-HLA-DR-CD33+cells directly correlated with the albumin levels (Rs=0.45; p=0.029). In «nonviral» group an inverse relationship was found between these indicators (Rs = -0.56; p=0.02), in addition the proportion of Lin-HLA-DR-CD33+and CD14+HLA-DRlow/-cells directly correlated with disease severity scores (Child-Pugh and MELD) direct correlation of the proportion of Lin-HLA-DR-CD33+and CD14+HLA-DRlow/-cells with the disease severity scores (Child-Pugh and MELD). Comparison of the initial content of myeloid suppressors with the response to complex therapy (that included autologous bone marrow-derived cell transplantation), showed that in viral liver cirrhosis, the proportion of Lin-HLA-DR-CD33+cells was characterized by a prognostic significance and, at values
Elastic ear cartilage of Acomys mice is recovering after injury
Authors: Bilyalov A. I , Filimoshina D. D , Filatov N. S , Bilyalova A. A , Titova A. A , Gataullina L. R , Plushkina A. S , Shagimardanova E. I , Deev R. V , Kiyasov A. P , Kozlova O. S , Nesmelov A. A , Gusev O. A
Published: February 2023

The article presents data on the regeneration of the auricle tissues of Acomys cahirinus mice (n=12) and Balb/c mice (n=12). Two experimental models were used: a full-thickness perforated defect with a diameter of 3 mm and subtotal removal of the auricle. Macrophotography and histological examination using general histological stains and detection of elastic fibers were performed after 2, 5, 15, 21, 30, 60, 90, and 120 days. It was found that regeneration in Acomys is more pronounced, which manifested itself in the complete elimination of the defect by 30-60 days in contrast to the control (Balb/c). A feature of this was less pronounced post-traumatic inflammation in Acomys mice. It was established the growth of a full-fledged auricle in case of its surgical removal. An important feature of regeneration in this case is the development of new cartilage tissue both from the edges of the original cartilage and developed in the form of separate islands of cartilage tissue. The newly formed cartilage was characterized by high cel-lularity, a smaller volume of the matrix, in the structure of which elastic fibers were formed. However, it should be noted that within 30-60 days. the recovery process, apparently, is not completed, the formed tissue regenerate enters the remodeling phase.
Structural and functional transformations of the brain in experimental mild traumatic brain injury
Authors: Plekhova N. G , Radkov I. V , Zinoviev S. V , Shumatov V. B
Published: February 2023

In mild traumatic brain injury, it is of interest to study neurode-generative conditions resulting from inflammatory changes in the nervous tissue. Purpose of the study: in the acute period in case of mild experimental traumatic brain injury, to reveal structural transformations of the nervous tissue of the brain. A modified model of a falling weight was used to reproduce of these trauma in adult rats. An immunohistochemical study of the brain with using rat-specific monoclonal antibodies to endothelin-1, glial fibrillar acidic protein, vimentin, and blood-brain barrier endothelial protein (SMI 71) was performed. It has been established that on the first day after injury in the cerebral cortex of animals, the spasm of blood vessels with capillary ischemia predominates. On day 8, there is an increase in the number of hyper- and hypochromic neurons, and after 14 days, restoration of the tone of the microcircu-latory bed is detected with signs of a violation of the permeability of the blood-brain barrier. A significant redistribution in the tissues of the cerebral hemispheres of glial elements containing acid glial protein and vimentin, as well as neurons producing endothelin-1, was noted. In the delayed post-traumatic period, compensatory reactions of the nervous tissue were revealed, which are characterized by the presence of morphological changes in neurons (an increase in the diameter and number of nucleoli in size) associated with intracellular regeneration, as well as the synthesis of various protein factors in them. Thus, the pathogenesis of mild experimental craniocerebral injury in the ischemic (1 day) and intermediate (8 days) periods is characterized by the presence of mild violations of the structural integrity of the nervous tissue of the brain. In the late post-traumatic period (14 days), neurons and astrocytes exhibit compensatory reactions.
Comparison of histo- and organogenesis of human pancreas, white laboratory mouse and spiny mouse (Acomys)
Authors: Sultanova K. N. , Titova A. A. , Plushkina A. S. , Andreeva D. I. , Kiyasov A. P.
Published: February 2023

The study of the embryonic development of the pancreas gives the opportunity to understand the mechanisms of organ regeneration in case of various pathologies. Worldwide research works, studying histo- and organogenesis of human pancreas, are based on data, received from model animals. Numerous processes of pancreatic development take several hours and remain unclear because white laboratory mouse has short gestation period. Spiny mouse (Acomys) has the prolonged prenatal period and can be a convenient model to study the stages of histo- and organogenesis of the pancreas. The review analyzed similarities and differences in the structure of human pancreas, white laboratory mouse and spiny mouse, the features of prenatal histo- and organogenesis of the pancreas, which should be considered in conducting and interpreting results of fundamental research, and possibility of using of spiny mice as a model animal to study embryonic development and pathology of the pancreas.
Angiogenic properties of glial progenitor cells derived from human induced pluripotent stem cells
Authors: Salikhova D. I. , Khaerdinova L. R. , Makhnach O. V. , Goldshtein D. V.
Published: February 2023

Diseases associated with impaired blood supply to the brain ranks second term of mortality in the world, losing the place only to coronary heart disease. The incidence of this disease in the world remains high and increasing significantly with the age. The recent years special attention has been paid to the search for new methods of therapy for ischemic diseases, such as study of angiogenic properties of stem cells and their conditioned medium. The aim of this work is studying the angiogenic properties of glial progenitor cells derived from human induced pluripotent stem cells. The study was carried out by testing the proliferative activity, mobility, migration of endothelial cells line EA.hy926 under the influence of glial progenitor cells and their conditioned medium. Also the research was conducted by ability to formation of the tubular and capillary-like structure by cells line EA.hy926 by modeling angiogenesis in the basement membrane matrix in vitro. The conditioned medium obtained by glial progenitor cells at concentrations of total protein 1 and 5 g/ml has a positive influence on the proliferative activity and mobility of the endothelial cells line EA.hy926. At the same time it does not accelerate the formation of the primary tubular and capillary-like structure by the modeling angiogenesis in the basement membrane matrix in vitro. But glial progenitor cells contribute to the formation of tubular and capillary-like structure due to contact-dependent signaling between the two cell types. The primary formed tubular structure had a long processes and large branch points under co cultivation with glial progenitor cells. Sprouting centers also had long and more convoluted processes and large cell clusters during the formation of a capillary-like structure. The glial progenitor cells and their conditioned medium had a positive effect on endothelial cell migration. This effect probably indicated by the production of substances by glial progenitor cells which was chemoattractants for endothelial cells line EA.hy926.
TB skin test recombinant proteins as vaccine candidates
Authors: Krasilnikov I. V. , Vinogradova T. I. , Djonovic M. , Zabolotnykh N. V. , Arakelov S. A. , Dogonadze M. Z. , Lunin V. G.
Published: February 2023

Incomplete protection of BCG vaccines, high variability of tuberculosis strains, together with the growing antibiotic resistance of mycobacterium tuberculosis, actualize the need to develop new anti-tuberculosis vaccines. Several novel experimental candidate vaccines based on recombinant proteins, such as those based on the M. tuberculosis ESAT-6 and CFP-10 antigens, are currently being studied in clinical trials. The genome region coding for ESAT-6 and CFP-10 antigens is deleted in BCG strains, so the BCG-immunized individuals cannot develop an immune response against the recombinant ESAT-6/CFP-10 antigen. Therefore, a positive immune reaction to these antigens in TB tests indicates the tested individual has earlier been exposed to M. tuberculosis. The ESAT-6/CFP-10 fusion recombinant antigen was, thus, selected as an immunogen to be evaluated on its potential to induce protective immunity against tuberculosis in a mice model when combined with a birch bark betulin-based vaccine adjuvant. The effect of use was assessed based on the results of histological evaluation of the infected lung tissue in mice and the Mtb lung content. The results herein reported eventually demonstrated that the use of corpuscular adjuvant-based (betulin) ESAT-6/CFP-10 vaccine preparation can induce the immune response commensurate to that of when immunized with the BCG vaccine.
Influence of some metabolites from plants of the genus Petasítes sp. on cancer cells motility in vitro
Authors: Filippova S. Yu. , Shamova T. V. , Timofeeva S. V. , Sitkovskaya A. O. , Mezhevova I. V. , Gnennaya N. V. , Novikova I. A. , Enin Ya. S. , Burov O. N. , Zlatnik E. Yu. , Mezentsev S. S. , Chernikova E. N. , Pandova O. V. , Pozdnyakova V. V. , Ezhova M. O. , Bakulina S. M. , Khokhlova O. V.
Published: February 2023

Secondary plant metabolites are a promising source of anticancer drugs. Of particular interest are substances that can reduce the migration activity of cancer cells as potential anti-metastatic drugs. In present work we study the influence of 2,4-dihydroxy-2,5-dimethylfuran-3(2H)-one, 5-(hydroxymethyl)furan-2-carbaldehyde and corinan obtained from plants of the genus Petastes sp. on scratch healing rate of permanent cancer lines PC3, A431, CaCo2, HeLa, and T98G. Cells were grown in DMEM medium supplemented with 10% FBS. To perform a scratch test, cells were planted in an amount of 1,5105 per well of a 24-well plate. After cell adhesion, a vertical scratch was applied to the cell monolayer with a plastic tip, after which the medium containing the test substances at a concentration of 40 M was added. Then, within 48 hours, photographing and determining the scratch area were carried out. The degree of scratch overgrowth was determined as the ratio of the difference between the scratch areas after 48 hours of cultivation and the scratch area at the initial moment, expressed as a percentage. As a result of the experiment, it was shown that 2,4-dihydroxy-2,5-dimethylfuran-3(2H)-one downregulates scratch healing rate in cultures A431, HeLa, T98G. The high sensitivity of A431 to corinan was also shown.
ESTÁGIO SUPERVISIONADO: DESAFIOS DA DOCÊNCIA EM MÚSICA NO ENSINO MÉDIO
Authors: Sousa Lima Thauana
Published: February 2023

O presente trabalho é um relato de experiência que discorre sobre o conceito e os desafios do Estágio Supervisionado no ensino de Música, envolvendo a relação entre Universidade – Escola – Acadêmico. Também aborda sobre a importância da experiência do estágio e sua influência na formação da personalidade do futuro docente em música. Dessa forma, esse artigo tem como objetivo a reflexão sobre a importância do Estágio Supervisionado e seus desafios, tendo como alvo o Estágio Supervisionado do Curso de Licenciatura em Linguagens e Códigos - Música, do Centro de Ciências São Bernardo, da Universidade Federal do Maranhão, realizado no Ensino Médio, na escola Centro de Ensino Prefeito Dionilo Gonçalves Costa, na cidade de Magalhães de Almeida - MA. Por meio das atividades descritas neste trabalho consideramos o estágio como uma atividade que junta as habilidades e os conhecimentos obtidos durante o curso, tornando-se desafiador para o discente, o futuro docente. Consideramos de extrema importância a interação entre Universidade e Escola nesse processo. Como resultado observamos a ausência da disciplina de Música no Ensino Básico das escolas da região, fazendo com que as aulas de Artes, majoritariamente sejam na linguagem das Artes Visuais, tornando um obstáculo para o estagiário de licenciatura em Música.
Students’ Perceptions of the Heat and Temperature Concepts: A Comparative Study between Primary, Secondary, and University Levels
Authors: Kotsis Konstantinos T. , Stylos Georgios , Pinelopi Houssou  , Kamaratos Matthaios
Published: February 2023

One of the fundamental problems that can impede the achievement of a sound understanding of a scientific concept is misconception. This study examines students’ perceptions of the concepts related to heat and temperature and how they evolve during the various levels of education. The findings from the literature review of students’ perceptions of heat issues combined with the results of this research, as obtained through the responses of students of primary, secondary, and tertiary education in Greece regarding the problems of temperature and heat, permitted the identification of alternative ideas of students for this conceptual area.
PERAN CUSTOMER INVOLVEMENT TERHADAP KINERJA PEMASARAN
Authors: Arifin Samsul , Ali Ali
Published: February 2023

Penelitian ini dilatarbelakangi oleh pertumbuhan bisnis di era globalisasi diikuti oleh persaingan bisnis, kinerja bisnis, dan kinerja pemasaran yang dilakukan oleh perusahaan mebel semakin dinamis. Persaingan dalam industri mebel berlangsung ketat dengan tingkat persaingan cukup tinggi. Berdasarkan kenyataan tersebut sebagai seorang peneliti tertarik untuk meneliti menguji inovasi pemasaran, orientasi pasar, kapabilitas pemasaran dan customer involvement mempunyai pengaruh terhadap kinerja pemasaran, namun peneliti menengarai ada pengaruh tidak langsung antara inovasi pemasaran, orientasi pasar, dan kapabilitas pemasaran melalui customer involvement terhadap kinerja pemasaran. Populasi pada penelitian ini adalah perusahaan mebel yang melakukan ekspor di Kabupaten Jepara yang berjumlah 307 perusahaan. Teknik pemilihan sampel dalam penelitian ini dilakukan dengan teknik probability sampling. Hasil dari penelitian ini menunjukkan bahwa inovasi pemasaran, orientasi pasar, kapabilitas pemasaran dan customer involvement berpengaruh signifikan terhadap kinerja pemasaran pada perusahaan mebel ekspor di Kabupaten Jepara. Penelitian selanjutnya, bisa dilakukan di semua klaster perusahaan mebel yaitu kluster perusahaan lokal, kluster perusahaan ekspor, dan kluster perusahaan PMA (Penanaman Modal Asing).
The Semantic and Pragmatic Functions of /bʕdɪn/ “بعدين” in Informal Arabic
Authors: A. Alqahtani Fatmah
Published: February 2023

This study aims at investigating the functions of the informal Arabic discourse marker “بعدين” /bʕdɪn/. By figuring out these functions and highlighting the common used ones, this paper tries to help communicators to be aware of those functions in their communication and avoid any possibility of misunderstanding. “Twitter” was the main tool of collecting the data of this study. This social medium provided natural communication contexts that significantly assisted the analysis of this study. The dataset consisted of one hundred and five tweets containing /bʕdɪn/. Those tweets reflected texts written by native Arabic speakers from different nationalities. The analysis was guided by the relevance theory and the methodology of related literature. The findings of the study showed that /bʕdɪn/ has semantic meanings similar to the English adverbs “then” and “later.” It was used semantically as a temporal adverb putting actions in sequence or delaying a move to unspecified time in the future. /bʕdɪn/ was also used as a coherence marker supporting the value of the communicative situations. In this respect and based on the collected data, seven pragmatic functions were elicited in this study for /bʕdɪn/. Those functions were: a marker of orientation shift, a marker of result, a conditional marker, a marker of disagreement, a coordination marker meaning “but,” a marker of agreement and a marker of reason.
World Literature Representation via Video Games
Authors: Alshhre Ali
Published: February 2023

This article aims to answer the following question: can video games be a dynamic medium for globalizing an adapted work of literature to be later played and received by gamers who might learn and increase their literary and cultural knowledge? World literature scholarship, gamer-response theory, game studies research, and my experience of playing Assassin’s Creed (2007) were used to explore how video games can globalize world literature to increase readership. The video game Assassin’s Creed (2007), adapted from Bartol’s Alamut (1938), was used to both discuss how world literature can be transmitted via video games and demonstrate how video game adaptations can be beneficial for learning and increasing global readership among gamers. The process of globalizing Alamut via Assassin’s Creed is shown by focusing on how the game employs different themes taken from the novel, its narrative, and some actual historical characters and places, such Acre, Jerusalem, Damascus, and Masyaf castle. Using literary novels and adapting them as video games can be adequate for incorporating those novels into world literature because the novel’s narrative is transmitted and exposed to gamers worldwide, thereby facilitating the transmission process of the novel’s content. Thus, it is recommended that the possibility of expanding world literature should be explored via adaptations of novels into video games.
Girls of Riyadh Revisited: Investigating the Quality of the English Collaborative Translation of Rajaa Alsanea’s Arabian Novel
Authors: Khuddro Ahmad
Published: February 2023

The paper aims to assess the quality of the English collaborative translation of Rajaa Alsanea’s Arabian novel Girls of Riyadh, focusing on linguistic and cultural features of the Target Text, a few of which are sometimes nonexistent in the Source Text. The mixed literal/functional approach to collaborative translation seems to be inconsistent, the paper observes, and it is possibly due to both the apparent tension between the self-translator and her co-translator Marilyn Booth and the prominence of the target culture sometimes recurrent in the Source Text. The paper addresses two questions: To what extent has collaborative translation affected the quality of rendering linguistic, social, and cultural references of the Source Text, such as idiomatic expressions, lexical terms, songs, names of celebrities, religious, and literary references, and traditional festivities? How effective have the literal and functional approach been in this collaborative translation? The findings of this study show that the translation procedures employed, such as omission, addition, and alteration, are sometimes unacceptable. Significantly, the study raises awareness about how the potential tension between the self-translator and the co-translator in the inclusion and exclusion of certain parts of texts can affect the quality of the end product. This paper recommends a fresh ‘critical translation’ of the ST that fills specific points which has been overlooked in the current ‘simple translation’: i.e., by paying attention to the message and intention of the author and suggesting to the use of different procedures by the two translators. The paper recommends that instead of implementing erratically two opposite approaches, the functional and literal approaches, as has been done, collaborative translators should follow one of them only. The current study suggests potential solutions to improve the quality of the English translation of this novel.
Osmotic pump maintains plasma concentrations of meloxicam for 6 days after orthopedic surgery in pigeons (Columba livia)
Authors: Coutant Thomas , Cococcetta Ciro , Prouillac Caroline , Espana Bernadette , Le Barzic Cécile , Arné Pascal , Huynh Minh
Published: February 2023

OBJECTIVE

To determine the plasma concentration of meloxicam delivered via an osmotic pump in pigeons undergoing orthopedic surgery and if an osmotic pump is a suitable alternative to repeated oral administration of this drug.

ANIMALS

16 free-ranging pigeons presented for rehabilitation with a wing fracture.

PROCEDURES

An osmotic pump filled with 0.2 mL of 40 mg/mL meloxicam injectable solution was implanted subcutaneously in the inguinal fold of 9 pigeons under anesthesia for orthopedic surgery. The pumps were removed 7 days postsurgery. Blood samples were collected before pump implantation (time 0) and 3, 24, 72, and 168 hours after pump implantation in 2 pigeons in a pilot study then at 12, 24, 72, and 144 hours in the 7 pigeons of the main study. The blood of 7 other pigeons receiving meloxicam at 2 mg/kg, PO, every 12 hours was also sampled between 2 to 6 hours after the last meloxicam administration. Plasma meloxicam concentrations were measured via high-performance liquid chromatography.

RESULTS

The plasma concentration of meloxicam was maintained at significant levels from 12 hours to 6 days after osmotic pump implantation. Median and minimum plasma concentrations in implanted pigeons were maintained at the same or higher level than those measured in pigeons that received meloxicam at a dose known to be analgesic in this species. No adverse effects attributable to either osmotic pump implantation and removal or meloxicam delivery were observed in this study.

CLINICAL RELEVANCE

Plasma concentrations levels of meloxicam in pigeons implanted with osmotic pumps were maintained at a similar concentration or higher than the suggested analgesic meloxicam plasma concentration in this species. Thus, osmotic pumps could represent a suitable alternative to the frequent capture and handling of birds for analgesic drug administration.
Arbuscular mycorrhizal fungi induced different proline accumulations in two sorghum accessions in a response to drought stress
Authors: Idris Idris , Fefirenta Agusdin Dharma , Sari Vega Kartika , Sudiana I Made
Published: February 2023

Sorghum has good adaptability to drought stress conditions, but its early vegetative phase and the generative phase are susceptible to stress. Understanding the physiological response of plants under drought and mechanisms regulating drought tolerance in a plant, mediated by arbuscular mycorrhizal fungi (AMF) will be useful in developing a strategy to deal with drought. Here, a pot experiment was used to explore the growth performance, biomass production and physiological responses of two sorghum accessions (4183A and JP-1) inoculated by the AMF under drought stress, as well as the effect of AMF on soil enzyme and microbial stability. Based on growth observations, the AMF inoculation treatment had not significant effect on increasing the drought resistance of the two sorghum accessions. Drought stress decreased the rate of height increment for 4183A, and JP-1 accessions by 37% and 55%, respectively, compared to normal conditions. Shoot dry weight and root dry weight losses were up to 59% and 66%, respectively, compared to well-watered conditions. However, the interaction of AMF and plants to deal with drought can be captured through physiological response, particularly proline accumulation. AMF inoculation in JP-1 accession reduced proline accumulation (99.91 mM/leaf fresh weight) compared to non-AMF inoculated plants (149.86 mM/leaf fresh weight). It can be implied that mycorrhiza can reduce plant stress. In contrast to accession 4183A, there was an increase in the accumulation of proline in plants inoculated with mycorrhiza under drought conditions. Additionally, AMF inoculation improved acid phosphatase activity in the soil and proved crucial for maintaining the stability of the rhizosphere microorganisms under drought-stressed conditions.
Cost Effectiveness of the Zero-Net Energy Passive House
Authors: Kołodziejczyk-Kęsoń Ada , Grebski Michalene
Published: February 2023

The authors main idea is to build energy plus buildings by means of architectural processes to design passive houses according to the Darmstadt Standard with additional equipment powered by renewable energy. The article presents a comparative financial analysis of passive house buildings with an additional source of power to charge electric cars in Poland and the United States. The analysis is based on market data while also taking into consideration inflation and increases in energy costs. The methodology is based on the calculations required by USA banks for obtaining credit. The emphasis is also on the distinction between commonly used term passive house and the term passive house in accordance with the Darmstadt Standard. The authors are introducing a new terminology to define buildings wrongly named as “passive”. Now Authors will name them Pseudo-Passive Buildings. The research shows that it is profitable to construct nearly zero-energy buildings with photovoltaic installation and the discounted rate on return of investment does not exceed the mortgage length.
Visual Solutions as a Way to Improve Work Safety When Using Machines – Selected Aspects of VM
Authors: Małysa Tomasz , Furman Joanna
Published: February 2023

The issue of ensuring work safety during the use of machines plays a key role due to the recorded accident events, the source of which are the machines in use. In the scope of reducing the risk associated with machines, particular attention should be paid to the threats, as well as solutions allowing to limit their negative impact on the operator. The study presents the possibility of using visual management (VM) as a form of information transfer that allows to meet the requirements set out in legal regulations, as well as reduce the risk of accidents. The machines in question were assessed for the possibility of using various forms of visual management to reduce the risk of accidents. The investigations were also supplemented with an analysis of accident statistics to present the importance of using visual management in improving the safety of machine operators' work. The conducted analyzes allowed to determine the direction of activities in the use of various forms of visual management aimed at improving the safety of machine operators.
Encapsulation of hydroxycitronellal in β-cyclodextrin and the characteristics of the inclusion complex
Authors: Zhu Guangyong , Xiao Zuobing , Zhou Rujun , Liu Junhua , Zhu Guangxu
Published: February 2023

Hydroxycitronellal has been widely used in foods, beverages, perfumery and cosmetics. It can also be used to treat anxiety. The major drawbacks regarding the use of hydroxycitronellal are related to water insolubility, volatility, instability, and sensitization. To overcome these concerns, β-cyclodextrin was adopted as wall material to encapsulate hydroxycitronellal in this work. Hydroxycitronellal-β-cyclodextrin inclusion complex was prepared and the product was characterized. The interaction of hydroxycitronellal and β-cyclodextrin, and the assembly of hydroxycitronellal-β-cyclodextrin inclusion complex were investigated by molecular simulation (MM). The results showed that hydroxycitronellal loading capacity was 8.5%. The thermal stability and lastingness of hydroxycitronellal were improved by the formation of the inclusion complex. The minimum binding energy was –151.2 kJ/mol. Among the perpendicular, staggered parallel and ideally parallel orientation of the inclusion complexes, the minimum energy value was found for the staggered parallel arrangement. These basic data are useful to understand the interaction between hydroxycitronellal and β-cyclodextrin.
Head and neck cancers of unknown primary: A diagnostic and therapeutic challenge
Authors: Enache Raluca , Sarafoleanu Dorin , Sarafoleanu Codrut
Published: February 2023

Head and neck cancers of unknown primary (CUP) represent up to 10% of all cancers located in the head and neck. True cancers of unknown primary, which remain of undetected primary origin after a correct clinical, imaging, surgical evaluation, have a reported frequency of between 1% and 2%. Cancers of unknown primary of the head and neck are a diagnostic challenge, considering that their main clinical manifestation is represented by the appearance of lymph node metastases in the cervical region.

In general, the detection of cancers with an unknown starting point is late, difficult, with significant therapeutic failure. The diagnostic-therapeutic approach starts from the histopathological structure of the lymph node metastasis and continues with a correct and complete imaging evaluation.

In this review are presented the diagnosis and therapeutic challenges and importance of head and neck cancers with unknown primary.