bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effectiveness of Electroacupuncture for Functional Constipation: A Randomized, Controlled, Clinical Trial
Authors: Da Nili , Wang Xinjun , Liu Hairong , Xu Xiuzhu , Jin Xun , Chen Chaoming , Zhu Dan , Bai Jiejing , Zhang Xiaoqing , Zou Yangyang , Hu Guangyong , Zhang Jianbin
Published: May 2015

Background. Electroacupuncture (EA) has been reported to treat functional constipation (FC). The aim of this study was to investigate the efficacy and safety of EA with different needle insertion method for FC.Methods. Sixty-seven participants were randomly assigned to control (EA with shallow puncture) and EA (with deep puncture) groups. Every patient received 5 treatments per week in the first two weeks, then 3 treatments per week during the following six weeks. Complete spontaneous bowel movements (CSBM), spontaneous bowel movements (SBM), Bristol stool scores (BSS), and Patient Assessment of Constipation Quality of Life (PAC-QOL) were assessed.Results. Both shallow and deep EA significantly increased CSBM frequency compared to the baseline. CSBM was increased from 0.50 ± 0.59/wk to 2.00 ± 1.67/wk with deep EA and from 0.48 ± 0.59/wk to 1.33 ± 1.09/wk with shallow EA (P< 0.05, resp.). Similar finding was noted in SBM. Deep EA was more potent than shallow EA (P< 0.05) during the treatment period. No difference was found on BSS and PAC-QOL between two groups.Conclusion. It is effective and safe with EA to treat FC. Studies with large sample size and long-term observation are needed for further investigation.
Curcumin Pharmacokinetic and Pharmacodynamic Evidences in Streptozotocin-Diabetic Rats Support the Antidiabetic Activity to Be via Metabolite(s)
Authors: Gutierres Vânia Ortega , Campos Michel Leandro , Arcaro Carlos Alberto , Assis Renata Pires , Baldan-Cimatti Helen Mariana , Peccinini Rosângela Gonçalves , Paula-Gomes Silvia , Kettelhut Isis Carmo , Baviera Amanda Martins , Brunetti Iguatemy Lourenço
Published: May 2015

This study measures the curcumin concentration in rat plasma by liquid chromatography and investigates the changes in the glucose tolerance and insulin sensitivity of streptozotocin-diabetic rats treated with curcumin-enriched yoghurt. The analytical method for curcumin detection was linear from 10 to 500 ng/mL. TheCmax⁡and the time to reachCmax⁡(tmax⁡) of curcumin in plasma were 3.14 ± 0.9 μg/mL and 5 minutes (10 mg/kg, i.v.) and 0.06 ± 0.01 μg/mL and 14 minutes (500 mg/kg, p.o.). The elimination half-time was 8.64 ± 2.31 ​​(i.v.) and 32.70 ± 12.92 (p.o.) minutes. The oral bioavailability was about 0.47%. Changes in the glucose tolerance and insulin sensitivity were investigated in four groups: normal and diabetic rats treated with yoghurt (NYOG and DYOG, resp.) and treated with 90 mg/kg/day curcumin incorporated in yoghurt (NC90 and DC90, resp.). After 15 days of treatment, the glucose tolerance and the insulin sensitivity were significantly improved in DC90 rats in comparison with DYOG, which can be associated with an increase in the AKT phosphorylation levels and GLUT4 translocation in skeletal muscles. These findings can explain, at least in part, the benefits of curcumin-enriched yoghurt to diabetes and substantiate evidences for the curcumin metabolite(s) as being responsible for the antidiabetic activity.
CD40 Ligand Deficient C57BL/6 Mouse Is a Potential Surrogate Model of Human X-Linked Hyper IgM (X-HIGM) Syndrome for Characterizing Immune Responses against Pathogens
Authors: Lopez-Saucedo Catalina , Bernal-Reynaga Rodolfo , Zayas-Jahuey Jesus , Galindo-Gomez Silvia , Shibayama Mineko , Garcia-Galvez Carlos , Estrada-Parra Sergio , Estrada-Garcia Teresa
Published: May 2015

Individuals with X-HIGM syndrome fail to express functional CD40 ligand; consequently they cannot mount effective protective antibody responses against pathogenic bacteria. We evaluated, compared, and characterized the humoral immune response of wild type (WT) and C57-CD40L deficient (C57-CD40L−/−) mice infected withCitrobacter rodentium. Basal serum isotype levels were similar for IgM and IgG3 among mice, while total IgG and IgG2b concentrations were significantly lower in C57-CD40L−/−mice compared with WT. Essentially IgG1 and IgG2c levels were detectable only in WT mice. C57-CD40L−/−animals, orally inoculated with2×109CFU, presented several clinical manifestations since the second week of infection and eventually died. In contrast at this time point no clinical manifestations were observed among C57-CD40L−/−mice infected with1×107CFU. Infection was subclinical in WT mice inoculated with either bacterial dose. The serum samples from infected mice (1×107CFU), collected at day 14 after infection, had similarC. rodentium-specific IgM titres. Although C57-CD40L−/−animals had lower IgG and IgG2b titres than WT mice, C57-CD40L−/−mice sera displayed complement-mediated bactericidal activity againstC. rodentium.C. rodentium-infected C57-CD40L−/−mice are capable of producing antibodies that are protective. C57-CD40L−/−mouse is a useful surrogate model of X-HIGM syndrome for studying immune responses elicited against pathogens.
Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis
Authors: O’Valle Francisco , Peregrina Magdalena , Crespo-Lora Vicente , Galindo-Moreno Pablo , Roman Maria , Padial-Molina Miguel , Mesa Francisco , Aneiros-Fernandez Jose , Aguilar David , Gonzalez-Rey Elena , Delgado Mario , Hernandez-Cortes Pedro
Published: May 2015

Background. Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein.Methods. CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1.Results. Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization (P<0.001). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions (P<0.0001) and was related to the severity of the lesions (Spearman’s rho = 0.775,P=0.0001).Conclusions. Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair.
Therapeutic Effects of Oligonol, Acupuncture, and Quantum Light Therapy in Chronic Nonbacterial Prostatitis
Authors: Öztekin İlhan , Akdere Hakan , Can Nuray , Aktoz Tevfik , Arda Ersan , Turan Fatma Nesrin
Published: May 2015

This research aimed to compare anti-inflammatory effects of oligonol, acupuncture, and quantum light therapy in rat models of estrogen-induced prostatitis. Adult male Wistar albino rats were grouped as follows: Group I, control (n= 10); Group II, chronic prostatitis (n= 10); Group III, oligonol (n= 10); Group IV, acupuncture (n= 10); Group V, quantum (n= 10); Group VI, oligonol plus quantum (n= 10); Group VII, acupuncture plus oligonol (n= 10); Group VIII, quantum plus acupuncture (n= 10); and Group IX, acupuncture plus quantum plus oligonol (n= 10). Chronic prostatitis (CP) was induced by the administration of 17-beta-estradiol (E2) and dihydrotestosterone (DHT). Oligonol was given for 6 weeks at a dose of 60 mg/day. Acupuncture needles were inserted at CV 3/4 and bilaterally B 32/35 points with 1-hour manual stimulation. Quantum therapy was administered in 5-minute sessions three times weekly for 6 weeks. Lateral lobes of prostates were dissected for histopathologic evaluation. Although all of the treatment modalities tested in this study showed anti-inflammatory effects in the treatment of CP in male rats, a synergistic effect was observed for oligonol plus quantum light combination. Monotherapy with oligonol showed a superior anti-inflammatory efficacy as compared to quantum light and acupuncture monotherapies.
Using a Prediction Model to Manage Cyber Security Threats
Authors: Jaganathan Venkatesh , Cherurveettil Priyesh , Muthu Sivashanmugam Premapriya
Published: May 2015

Cyber‐attacks are an important issue faced by all organizations. Securing information systems is critical. Organizations should be able to understand the ecosystem and predict attacks. Predicting attacks quantitatively should be part of risk management. The cost impact due to worms, viruses, or other malicious software is significant. This paper proposes a mathematical model to predict the impact of an attack based on significant factors that influence cyber security. This model also considers the environmental information required. It is generalized and can be customized to the needs of the individual organization.
Attenuating Immune Response of Macrophage by Enhancing Hydrophilicity of Ti Surface
Authors: Dai Xiaohan , Wei Yan , Zhang Xuehui , Meng Song , Mo Xiaoju , Liu Xing , Deng Xuliang , Zhang Li , Deng Xuming
Published: May 2015

Immune responses can determine the in vivo fate of implanted materials. The strategy for developing implants has shifted towards using materials with immunomodulatory activity. However, the immunoregulatory effect of hydrophilicity of titanium surface on the macrophage behavior and its underlying mechanism remain poorly understood. Here, the Ti surface hydrophilicity‐dependent behavior of murine RAW264.7 macrophages was investigated in vitro. Two laboratory models with significantly different surface hydrophilicity and similar roughness were established with Ti‐polished and Ti‐H2O2 surfaces. The results of cell morphology observation showed that the Ti‐H2O2 surface yielded enhanced cell adhesion and less multinucleated cell formation. CCK‐8 assay indicated that the growth rate of macrophage on Ti‐H2O2 surface is higher than that of Ti‐polished. ELISA assay result revealed lower level of proinflammatory factor TNF‐α and higher level of anti‐inflammatory factor IL‐10 on the Ti‐H2O2 surface compared to Ti‐polished. Subsequently, immunofluorescence and western blotting analysis showed that activation of the NF‐κB‐TNF‐α pathway might be involved in the modulation of the immune response by surface hydrophilicity. Together, these results suggested that relative high hydrophilic Ti surface might attenuate the immune response of macrophage by activating NF‐κB signaling. These findings could provide new insights into designing implant devices for orthopedic applications.
A Hybrid Measurement Approach to Medium Occupied Time for Radio Resource Management in IEEE 802.11 Networks
Authors: Yang Seung-Chur , Kim Jong-Deok
Published: May 2015

The wireless resources required by users have increased in parallel with and above improvements in WLAN technology, requiring even more accurate and practical resource management. Research on medium utilization, which is fundamental to resource management, has supplied insufficient evidence to apply it in real environments. This paper proposes a hybrid measurement approach to medium utilization using both a signal-level register monitor and a frame-level frame parser. We redefine medium utilization in terms of Medium Occupied Time (MOT) to describe the status of the medium in more detail. The proposed approach enables us to measure the classified MOT occupied by 802.11 frames, wireless interference, and a MAC protocol. We implement our approach by modifying a Linux device driver of an off-the-shelf 802.11n NIC. In addition, an indoor test bed is built to verify the approach's support for various traffic patterns, quantitative measurement of wireless interference, and backoff time estimation. We conclude that our hybrid measurement approach is accurate and deployable in a real environment.
An Activity of Thioacyl Derivatives of 4-Aminoquinolinium Salts towards Biofilm Producing and Planktonic Forms of Coagulase-Negative Staphylococci
Authors: Wojtyczka Robert D. , Zięba Andrzej , Dziedzic Arkadiusz , Kępa Małgorzata , Idzik Danuta
Published: May 2015

Microorganisms present in different environments have developed specific mechanisms of settling on various abiotic and biotic surfaces by forming a biofilm. It seems to be well justified to search for new compounds enabling biofilm reduction, which is highly resistant to antibiotics. This study was thus an initial assessment of the antibacterial activity of two new quinoline derivatives of a structure of 3-thioacyl 1-methyl 4-arylaminoquinolinium salts against coagulase-negative staphylococci (CoNS) isolated from a hospital environment, in a form of both biofilms and in planktonic form. Thirty-three stains of CoNS isolated from the hospital environment (air, surfaces) and seven reference strains from the ATCC collection were selected for the study. The mean MIC value for 1-methyl-3-benzoylthio-4-(4-chlorophenylamino)quinolinum chloride (4-chlorophenylamino derivative) was 42.60 ± 19.91 μg/mL, and in the case of strains subjected to 1-methyl-3-benzoylthio-4-(4-fluorophenylamino)quinolinum chloride (4-fluorophenylamino derivative) activity, the mean MIC value was 43.20 ± 14.30 μg/mL. The mean concentration of 4-chlorophenylamino derivative that inhibited biofilm formation was 86.18 ± 30.64 μg/mL. The mean concentration of 4-fluorophenylamino derivatives that inhibited biofilm formation was higher and amounted to 237.09 ± 160.57 μg/mL. Based on the results, both derivatives of the examined compounds exhibit high antimicrobial activity towards strains growing both in planktonic and biofilm form.
The TF-miRNA Coregulation Network in Oral Lichen Planus
Authors: Zuo Yu-Ling , Gong Di-Ping , Li Bi-Ze , Zhao Juan , Zhou Ling-Yue , Shao Fang-Yang , Jin Zhao , He Yuan
Published: May 2015

Oral lichen planus (OLP) is a chronic inflammatory disease that affects oral mucosa, some of which may finally develop into oral squamous cell carcinoma. Therefore, pinpointing the molecular mechanisms underlying the pathogenesis of OLP is important to develop efficient treatments for OLP. Recently, the accumulation of the large amount of omics data, especially transcriptome data, provides opportunities to investigate OLPs from a systematic perspective. In this paper, assuming that the OLP associated genes have functional relationships, we present a new approach to identify OLP related gene modules from gene regulatory networks. In particular, we find that the gene modules regulated by both transcription factors (TFs) and microRNAs (miRNAs) play important roles in the pathogenesis of OLP and many genes in the modules have been reported to be related to OLP in the literature.
Epidemiological Characterization of Drug Resistance amongMycobacterium tuberculosisIsolated from Patients in Northeast of Iran during 2012-2013
Authors: Tavanaee Sani Ashraf , Shakiba Abolfazl , Salehi Maryam , Bahrami Taghanaki Hamid Reza , Ayati Fard Seiedeh Fatemeh , Ghazvini Kiarash
Published: May 2015

Introduction. Tuberculosis is still one of the most important health problems in developing countries and increasing drug resistance is the main concern for its treatment. This study was designed to characterize the drug resistantMycobacterium tuberculosisisolated from patients suffering from pulmonary tuberculosis in northeast of Iran.Method. In this cross-sectional study during 2012-2013, drug susceptibility testing was performed onMycobacterium tuberculosisisolated in northeast of Iran using proportional method. Epidemiological data concerning these strains were also analyzed.Results. Among 125 studied isolates, 25 mycobacteria (20%) were diagnosed as nontuberculosis mycobacteria. Among the remaining 100Mycobacterium tuberculosisisolates, the resistance rates were 7%, 7%, 3%, and 9% against isoniazid, rifampin, ethambutol, and streptomycin, respectively. Four isolates were resistant against both isoniazid and rifampin (MDR tuberculosis). The highest resistance rate was observed among 15–45-year-old patients. The MDR tuberculosis was much more prevalent among those who had previous history of treatment.Conclusion. Considering these findings, DOTS strategy should be emphasized and promptly used in order to prevent further resistance. Regarding the high rate of nontuberculosis mycobacteria, it is recommended that confirmatory tests were performed before any therapeutic decision.
The Neuroprotective Effects ofRatanasampilon Oxidative Stress-Mediated Neuronal Damage in Human Neuronal SH-SY5Y Cells
Authors: Zhu Aiqin , Wu Zhou , Meng Jie , McGeer Patrick L. , Zhu Yi , Nakanishi Hiroshi , Wu Shizheng
Published: May 2015

We previously found thatRatanasampil(RNSP), a traditional Tibetan medicine, improves the cognitive function of mild-to-moderate AD patients living at high altitude, as well as learning and memory in an AD mouse model (Tg2576); however, mechanism underlying the effects of RNSP is unknown. In the present study, we investigated the effects and molecular mechanisms of RNSP on oxidative stress-induced neuronal toxicity using human neuroblastoma SH-SY5Y cells. Pretreatment with RNSP significantly ameliorated the hydrogen peroxide- (H2O2-) induced cytotoxicity of SH-SY5Y cells in a dose-dependent manner (up to 60 μg/mL). Furthermore, RNSP significantly reduced the H2O2-induced upregulation of 8-oxo-2′-deoxyguanosine (8-oxo-dG, the oxidative DNA damage marker) but significantly reversed the expression of repressor element-1 silencing transcription factor (REST) from H2O2associated (100 μM) downregulation. Moreover, RNSP significantly attenuated the H2O2-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase 1/2 (ERK 1/2) in SH-SY5Y cells. These observations strongly suggest that RNSP may protect the oxidative stress-induced neuronal damage that occurs through the properties of various antioxidants and inhibit the activation of MAPKs. We thus provide the principle molecular mechanisms of the effects of RNSP and indicate its role in the prevention and clinical management of AD.
Time Reversal Reconstruction Algorithm Based on PSO Optimized SVM Interpolation for Photoacoustic Imaging
Authors: Sun Mingjian , Wu Zhenghua , Liu Ting , Hu Jiajun , Wu Guanqun , Feng Naizhang
Published: May 2015

Photoacoustic imaging is an innovative imaging technique to image biomedical tissues. The time reversal reconstruction algorithm in which a numerical model of the acoustic forward problem is run backwards in time is widely used. In the paper, a time reversal reconstruction algorithm based on particle swarm optimization (PSO) optimized support vector machine (SVM) interpolation method is proposed for photoacoustics imaging. Numerical results show that the reconstructed images of the proposed algorithm are more accurate than those of the nearest neighbor interpolation, linear interpolation, and cubic convolution interpolation based time reversal algorithm, which can provide higher imaging quality by using significantly fewer measurement positions or scanning times.
Efficacy and Cost Effectiveness of the Acupuncture Treatment Using a New Skin Stimulus Tool CalledM-Test Which Is a Measure Based on Symptoms Accompanied with Body Movements: A Pragmatic RCT Targeting Hemodialysis Patients
Authors: Ono Shuji , Mukaino Yoshito
Published: May 2015

M-Test can simultaneously reduce hemodialysis patients’ diverse symptoms. Its diagnosis and treatment are based on simple movements that can be performed by anyone and allow determining which meridians have problems by analyzing symptoms accompanied with movement. It also enables to conduct a safe and effective treatment with use of microcorn which is a noninvasive treatment tool. This time we conducted microcorn intervention on hemodialysis patients based on diagnosis ofM-Test. As a result, almost all of the dialysis patients’ complaints have been relieved while the score of HR-QOL increased. According to our calculation of cost effectiveness, it confirmed that it is very cost-effective.
Bilateral Ureteral-Iliac Artery Fistula in a Patient with Chronic Indwelling Ureteral Stents: A Case Report and Review
Authors: Rafiei Arash , Weber Timothy A. , Kongnyuy Michael , Ordorica Raul
Published: May 2015

Ureteral-arterial fistula (UAF) is an exceedingly rare but life-threatening condition warranting emergent intervention. Prompt recognition and management of UAF in suspect patients presenting with gross hematuria are required for a successful outcome. We report a rare subset of UAF involving the bilateral common iliac arteries. The patient underwent successful endovascular stent-grafting to correct the arterial defect and delayed open repair of the ureteral strictures. Timely management has benefited from the collaboration of the involved medical teams, which included emergency medicine, urology, and interventional radiology.
Dye-Free Porcine Model of Experimental Branch Retinal Vein Occlusion: A Suitable Approach for Retinal Proteomics
Authors: Jørgensen Cehofski Lasse , Kruse Anders , Kjærgaard Benedict , Stensballe Allan , Honoré Bent , Vorum Henrik
Published: May 2015

Branch retinal vein occlusion induces complex biological processes in the retina that are generated by a multitude of interacting proteins. These proteins and their posttranslational modifications can effectively be studied using modern proteomic techniques. However, no method for studying large-scale protein changes following branch retinal vein occlusion has been available until now. Obtainment of retinal tissue exposed to branch retinal vein occlusion is only available through experimental animal models. Traditional models of experimental branch retinal vein occlusion require the use of Rose Bengal dye combined with argon laser photocoagulation. The use of Rose Bengal dye is problematic in proteomic studies as the dye can induce multiple protein modifications when irradiated. This paper presents a novel technique for proteomic analysis of porcine retinal tissue with branch retinal vein occlusion combining a dye-free experimental model with label-free liquid chromatography mass spectrometry based proteomics.
Improving Mechanical Properties of Thermoset Biocomposites by Fiber Coating or Organic Oil Addition
Authors: Ngo Truc T. , Kohl James G. , Paradise Tawni , Khalily Autumn , Simonson Duane L.
Published: May 2015

Two different thermoset biocomposite systems are experimented in this study with the hope to improve their mechanical properties. Fiberglass and hemp, in form of fabrics, are used to reinforce the thermoset polymer matrix, which includes a traditional epoxy resin and a linseed oil-based bioresin (UVL). The fiber/polymer matrix interface is modified using two different approaches: adding a plant-based oil (pine or linseed) to the polymer matrix or coating the fibers with 3-(aminopropyl)triethoxysilane (APTES) prior to integrating them into the polymer matrix. Epoxy resin is cured using an amine-based initiator, whereas UVL resin is cured under ultraviolet light. Results show that hemp fibers with APTES prime coat used in either epoxy or UVL matrix exhibit some potential improvements in the composite’s mechanical properties including tensile strength, modulus of elasticity, and ductility. It is also found that adding oil to the epoxy matrix reinforced with fiberglass mostly improves the material’s modulus of elasticity while maintaining its tensile strength and ductility. However, adding oil to the epoxy matrix reinforced with hemp doubles the material’s ductility while slightly reducing its tensile strength and modulus of elasticity.
Identification of Gene and MicroRNA Signatures for Oral Cancer Developed from Oral Leukoplakia
Authors: Zhu Guanghui , He Yuan , Yang Shaofang , Chen Beimin , Zhou Min , Xu Xin-Jian
Published: May 2015

In clinic, oral leukoplakia (OLK) may develop into oral cancer. However, the mechanism underlying this transformation is still unclear. In this work, we present a new pipeline to identify oral cancer related genes and microRNAs (miRNAs) by integrating both gene and miRNA expression profiles. In particular, we find some network modules as well as their miRNA regulators that play important roles in the development of OLK to oral cancer. Among these network modules, 91.67% of genes and 37.5% of miRNAs have been previously reported to be related to oral cancer in literature. The promising results demonstrate the effectiveness and efficiency of our proposed approach.
Innate and Adaptive Immunity in Calcific Aortic Valve Disease
Authors: Mathieu Patrick , Bouchareb Rihab , Boulanger Marie-Chloé
Published: May 2015

Calcific aortic valve disease (CAVD) is the most common heart valve disorder. CAVD is a chronic process characterized by a pathologic mineralization of valve leaflets. Ectopic mineralization of the aortic valve involves complex relationships with immunity. Studies have highlighted that both innate and adaptive immunity play a role in the development of CAVD. In this regard, accumulating evidence indicates that fibrocalcific remodelling of the aortic valve is associated with activation of the NF-κB pathway. The expression of TNF-αand IL-6 is increased in human mineralized aortic valves and promotes an osteogenic program as well as the mineralization of valve interstitial cells (VICs), the main cellular component of the aortic valve. Different factors, including oxidized lipid species, activate the innate immune response through the Toll-like receptors. Moreover, VICs express 5-lipoxygenase and therefore produce leukotrienes, which may amplify the inflammatory response in the aortic valve. More recently, studies have emphasized that an adaptive immune response is triggered during CAVD. Herein, we are reviewing the link between the immune response and the development of CAVD and we have tried, whenever possible, to keep a translational approach.
Association between Programmed Cell Death 6 Interacting Protein Insertion/Deletion Polymorphism and the Risk of Breast Cancer in a Sample of Iranian Population
Authors: Hashemi Mohammad , Yousefi Javad , Hashemi Seyed Mehdi , Amininia Shadi , Ebrahimi Mahboubeh , Taheri Mohsen , Ghavami Saeid
Published: May 2015

It has been suggested that genetic factors contribute to patients’ vulnerability to breast cancer (BC). The programmed cell death 6 interacting protein (PDCD6IP) encodes for a protein that is known to bind to the products of the PDCD6 gene, which is involved in the apoptosis pathway. The aim of this case-control study is to investigate the relationship between thePDCD6IP15 bp insertion/deletion (I/D) polymorphism (rs28381975) and BC risk in an Iranian population. A total of 491 females, including 266 BC patients and 225 control subjects without cancer, were enrolled into the study. Our findings revealed that thePDCD6IP15 bp I/D polymorphism decreased the risk of BC in codominant (OR=0.44, 95%CI=0.31–0.65,p<0.0001, I/D versus DD;OR=0.39, 95%CI=0.17–0.88,p=0.030, I/I versus DD) and dominant (OR=0.44, 95%CI=0.30–0.63,p<0.0001, D/I + I/I versus D/D) tested inheritance models. Also, thePDCD6IPI allele significantly decreased the risk of BC (OR=0.59, 95%CI=0.45–0.78,p<0.001) compared to the D allele.
Some Experimental Investigations on Gas Turbine Cooling Performed with Infrared Thermography at Federico II
Authors: Astarita T. , Cardone G. , de Luca L. , Carlomagno G. M.
Published: May 2015

This paper reviews some experimental measurements of convective heat transfer coefficient distributions which are connected with the cooling of gas turbines, performed by the authors’ research group at the University of Naples Federico II with infrared thermography. Measurements concern impinging jets, cooling of rotating disks, and gas turbine blades, which are either stationary or rotating. The heated thin foil sensor, associated with the detection of surface temperature by means of infrared thermography, is exploited to accurately measure detailed convective heat transfer coefficient maps. The paper also intends to show how to correctly apply the infrared technique in a variety of gas turbines cooling problems.
Death Receptor 3 (TNFRSF25) Increases Mineral Apposition by Osteoblasts and Region Specific New Bone Formation in the Axial Skeleton of Male DBA/1 Mice
Authors: Collins Fraser L. , Williams Jessica O. , Bloom Anja C. , Stone Michael D. , Choy Ernest , Wang Eddie C. Y. , Williams Anwen S.
Published: May 2015

Objectives. Genome wide association studies identified TNFSF member TNF-like protein 1A (TL1A, TNFSF15) as a potential modulator of ankylosing spondylitis (AS). TL1A is the only confirmed TNFSF ligand of death receptor 3 (DR3, TNFRSF25); however, its role in disease pathology is not characterised. We evaluated DR3’s role in controlling osteoblast- (OB-) dependent bone formationin vitroandin vivo.Methods. Osteoprogenitor cells and OB were cultured from male DR3-deficient (DR3ko) and wild-type (DR3wt) DBA/1 mice. DR3 and RANKL expression were tested by flow cytometry. Alkaline phosphatase and mineralization were quantified. Osteopontin, osteoprotegerin, and pro MMP-9 were measured by ELISA. A fluorescent probe (BoneTag) was used to measurein vivomineralization in 10-month-old mice.Results. DR3 was expressed on osteoprogenitors and OB fromDR3wtmice. Alkaline phosphatase, osteopontin, and mineral apposition were significantly elevated inDR3wtcultures. Levels of RANKL were comparable whilst osteoprotegerin was significantly increased inDR3wtcultures.In vivoincorporation of BoneTag was significantly lower in the thoracic vertebrae of 10-month-oldDR3komice.Conclusions. These data identify new roles for DR3 in regulating OB-dependent bone mineral apposition. They potentially begin to explain the atypical pattern of new bone formation observed in the axial skeleton of grouped, aging DBA/1 mice.
Physicochemical Quality Parameters at the Reception of the Honey Packaging Process: Influence of Type of Honey, Year of Harvest, and Beekeeper
Authors: Juan-Borrás Marisol , Domenech Eva , Conchado Andrea , Escriche Isabel
Published: May 2015

The aim of this paper was to evaluate the influence of the type of honey, year of collection, and the beekeeper on the main physicochemical quality parameters (hydroxymethylfurfural “HMF,” moisture, and colour), measured on reception of the raw honey. 1593 samples (11 types of honey categorized by means of pollinic analysis), provided by 98 beekeepers, from 2009 to 2013, were analyzed. Colour was the parameter most affected by the type of honey and year, whereas HMF was the least affected in both cases. The clearest honeys were found to have the greatest moisture (orange, rosemary, and lemon) and the darkest had the least moisture (lavender stoechas, eucalyptus, sunflower, honeydew and retama). Lavender, polyfloral, and thyme had intermediate values of these parameters. For moisture, most samples were in accordance with international requirements (less than 20 g/100 g). All values were below the required limit for HMF (40 mg/kg), although a few of them were abnormally high as they were raw honeys (i.e., 2% of the samples had values higher than 20 mg/kg). The fact that all the inadequate samples came from specific beekeepers highlights the importance of their role, suggesting that training in good practices is the key to guarantee honey quality before it reaches the industry.
miR-155 and miR-484 Are Associated with Time to Progression in Metastatic Renal Cell Carcinoma Treated with Sunitinib
Authors: Merhautova Jana , Hezova Renata , Poprach Alexandr , Kovarikova Alena , Radova Lenka , Svoboda Marek , Vyzula Rostislav , Demlova Regina , Slaby Ondrej
Published: May 2015

Background. Sunitinib is a tyrosine kinase inhibitor used in the treatment of metastatic renal cell carcinoma. The main difficulty related to the treatment is the development of drug resistance followed by rapid progression of the disease. We analyzed tumor tissue of sunitinib treated patients in order to find miRNAs associated with therapeutic response.Methods. A total of 79 patients with metastatic renal cell carcinoma were included in our study. miRNA profiling in tumor tissue samples was performed by TaqMan Low Density Arrays and a group of selected miRNAs (miR-155, miR-374-5p, miR-324-3p, miR-484, miR-302c, and miR-888) was further validated by qRT-PCR. Normalized data were subjected to ROC and Kaplan-Meier analysis.Results. We reported decreased tissue levels of miR-155 and miR-484 as significantly associated with increased time to progression (miR-155: median TTP 5.8 versus 12.8 months, miR-484: median TTP 5.8 versus 8.9 months).Conclusion. miR-155 and miR-484 are potentially connected with sunitinib resistance and failure of the therapy. miR-155 is a known oncogene with direct influence on neovascularization. Biological role of miR-484 has to be clarified. Stratification of patients based on miRNA analysis would allow more personalized approach in therapy of metastatic renal cell carcinoma.
Manifestation of Hyperandrogenism in the Continuous Light Exposure-Induced PCOS Rat Model
Authors: Kang Xuezhi , Jia Lina , Shen Xueyong
Published: May 2015

Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder, and its pathogenesis has yet to be completely clarified. A fully convincing animal model has not been established for PCOS. In earlier studies, researchers have shown that the exposure of rats to continuous light can induce PCOS; nevertheless, hyperandrogenism, a key characteristic observed in human PCOS, has not been reported previously. In the present study, we found that (1) body weights decreased in female rats in a continuous light environment with both ovarian and uterine augmentation; (2) the estrous cycle in rats under continuous light environment was disordered, and polycystic ovary-like changes occurred, accompanied with fur loss and lethargy; and (3) serum testosterone levels in rats in a continuous light environment significantly increased. Our data suggest that continuous light can lead to the occurrence of PCOS in female rats without the need for drugs; this is a reasonable PCOS animal model that is more consistent with the natural disease state in humans; and poor sleep habits or negligence of sleep hygiene may be an important lifestyle factor in pathogenesis of PCOS.
Mycobacterium-Host Cell Relationships in Granulomatous Lesions in a Mouse Model of Latent Tuberculous Infection
Authors: Ufimtseva Elena
Published: May 2015

Tuberculosis (TB) is a dangerous infectious disease characterized by a tight interplay between mycobacteria and host cells in granulomatous lesions (granulomas) during the latent, asymptomatic stage of infection.Mycobacterium-host cell relationships were analyzed in granulomas obtained from various organs of BALB/c mice with chronic TB infection caused byin vivoexposure to the Bacillus Calmette-Guérin (BCG) vaccine. Acid-fast BCG-mycobacteria were found to be morphologically and functionally heterogeneous (in size, shape, and replication rates in colonies) in granuloma macrophages, dendritic cells, and multinucleate Langhans giant cells. Cord formation by BCG-mycobacteria in granuloma cells has been observed. Granuloma macrophages retained their ability to ingest damaged lymphocytes and thrombocytes in the phagosomes; however, their ability to destroy BCG-mycobacteria contained in these cells was compromised. No colocalization of BCG-mycobacteria and the LysoTracker dye was observed in the mouse cells. Various relationships between granuloma cells and BCG-mycobacteria were observed in different mice belonging to the same line. Several mice totally eliminated mycobacterial infection. Granulomas in the other mice had mycobacteria actively replicating in cells of different types and forming cords, which is an indicator of mycobacterial virulence and, probably, a marker of the activation of tuberculous infection in animals.
Cognitive, Affective Problems and Renal Cross Ectopy in a Patient with 48,XXYY/47,XYY Syndrome
Authors: Resim Sefa , Kucukdurmaz Faruk , Kankılıc Nazım , Altunoren Ozlem , Efe Erkan , Benlioglu Can
Published: May 2015

Klinefelter syndrome is the most common sex chromosome abnormality (SCA) in infertile patients and 47,XXY genomic configuration constitutes most of the cases. However, additional Xs and/or Y such as 48,XXYY, 48,XXXY, and 47,XYY can occur less frequently than 47,XXY. Those configurations were considered as variants of Klinefelter syndrome. In this report, we present an infertile man with tall stature and decreased testicular volume. Semen analysis and hormonal evaluation supported the diagnosis of nonobstructive azoospermia. Genetic investigation demonstrated an abnormal male karyotype with two X chromosomes and two Y chromosomes consistent with 48,XXYY(17)/47,XYY (13). Additionally, the patient expressed cognitive and affective problems which were documented by psychomotor retardation and borderline intelligence measured by an IQ value between 70 and 80. Systemic evaluation also revealed cross ectopy and malrotation of the right kidney in the patient. The couple was referred to microtesticular sperm extraction (micro-TESE)/intracytoplasmic sperm injection (ICSI) cycles and preimplantation genetic diagnosis (PGD). To the best of our knowledge, this is the first report of combination of XYY and XXYY syndromes associated with cognitive, affective dysfunction and renal malrotation.
The Lipid Parameters and Lipoprotein(a) Excess in Hashimoto Thyroiditis
Authors: Yetkin D. O. , Dogantekin B.
Published: May 2015

Objective. The risk of atherosclerotic heart disease is increased in autoimmune thyroiditis, although the reason is not clear. Lipoprotein(a) (Lp(a)) excess has been identified as a powerful predictor of premature atherosclerotic vascular diseases. The aim of this study is to investigate the relationship between Lp(a) levels and thyroid hormones in Hashimoto patients.Method. 154 premenopausal female Hashimoto patients (48 patients with overthypothyroid (OH), 50 patients with subclinical hypothyroid (SH), and 56 patients with euthyroid Hashimoto to (EH)) were enrolled in this study. The control group consists of 50 age matched volunteers. In every group, thyroid function tests and lipid parameters with Lp(a) were measured. Lp(a) excess was defined as Lp(a) > 30 mg/dL.Results. Total-C, LDL-C, TG, and Lp(a) levels were increased in Hashimoto group. Total-C, LDL-C, and TG levels were higher in SH group than in the control group. Total-C and LDL-C levels were also higher in EH group compared to controls. Lp(a) levels were similar in SH and EH groups with controls. However, excess Lp(a) was more common in subclinical hypothyroid and euthyroid Hashimoto group than in the control group.Conclusion. The Total-C and LDL-C levels and excess Lp(a) were higher even in euthyroid Hashimoto patients. Thyroid autoimmunity may have some effect on Lp(a) and lipid metabolism.
Efficacy of Adaptive Biofeedback Training in Treating Constipation-Related Symptoms
Authors: Tang Jing , Huang Zhihui , Tan Yan , Zhang Nina , Tan Anping , Chen Jun , Chen Jianfeng
Published: May 2015

Biofeedback therapy is a well-known and effective therapeutic treatment for constipation. A previous study suggested that adaptive biofeedback (ABF) training was more effective than traditional (fixed training parameters) biofeedback training. The aim of this study was to verify the effectiveness of ABF in relieving constipation-related symptoms. We noticed that in traditional biofeedback training, a patient usually receives the training twice per week. The long training sessions usually led to poor compliance. This study proposes an intensive biofeedback therapy and compares intensive therapy with nonintensive therapy in patients with constipation-related symptoms.Methods.63 patients with constipation-related symptoms were treated with ABF between 2012 and 2013. These patients were further divided into the intensive therapy and nonintensive therapy groups.Results.A total of 63 patients were enrolled in the study, including 24 in the nonintensive therapy group and 39 in the intensive therapy group. 100%(N=21)of constipation patients achieved the primary efficacy endpoint (≥3 bowel movements/week). There was significant improvement in constipation-related symptoms after adaptive biofeedback. The intensive biofeedback therapy did not show better performance compared to nonintensive biofeedback therapy.Conclusions.This investigation provides support for the efficacy of biofeedback for constipation-related symptoms. The efficacy of intensive therapy is similar to nonintensive therapy.
A Review of Intra- and Extracellular Antigen Delivery Systems for Virus Vaccines of Finfish
Authors: Munang’andu Hetron Mweemba , Evensen Øystein
Published: May 2015

Vaccine efficacy in aquaculture has for a long time depended on evaluating relative percent survival and antibody responses after vaccination. However, current advances in vaccine immunology show that the route in which antigens are delivered into cells is deterministic of the type of adaptive immune response evoked by vaccination. Antigens delivered by the intracellular route induce MHC-I restricted CD8+ responses while antigens presented through the extracellular route activate MHC-II restricted CD4+ responses implying that the route of antigen delivery is a conduit to induction of B- or T-cell immune responses. In finfish, different antigen delivery systems have been explored that include live, DNA, inactivated whole virus, fusion protein, virus-like particles, and subunit vaccines although mechanisms linking these delivery systems to protective immunity have not been studied in detail. Hence, in this review we provide a synopsis of different strategies used to administer viral antigens via the intra- or extracellular compartments. Further, we highlight the differences in immune responses induced by antigens processed by the endogenous route compared to exogenously processed antigens. Overall, we anticipate that the synopsis put together in this review will shed insights into limitations and successes of the current vaccination strategies used in finfish vaccinology.
Attention Deficit Hyperactivity Disorder Symptoms, Comorbidities, Substance Use, and Social Outcomes among Men and Women in a Canadian Sample
Authors: Vingilis Evelyn , Erickson Patricia G. , Toplak Maggie E. , Kolla Nathan J. , Mann Robert E. , Seeley Jane , vanderMaas Mark , Daigle Deanne S.
Published: May 2015

Background.Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder that can persist in adolescence and adulthood.Aim.To examine prevalence of ADHD symptoms and correlates in a representative sample of adults 18 years and older living in Ontario, Canada.Method.We used the Centre for Addiction and Mental Health Monitor, an ongoing cross-sectional telephone survey, to examine the relationships between ADHD positive symptoms and comorbidities, substance use, medication use, social outcomes, and sociodemographics.Results.Of 4014 residents sampled in 2011-2012, 3.30% (2.75%–3.85%) screened positively for ADHD symptoms (women = 3.6%; men = 3.0%). For men, distress, antisocial symptoms, cocaine use, antianxiety medication use, antidepressant medication use, and criminal offence arrest were associated with positive ADHD screen. For women, distress, cocaine use, antianxiety medication use, antidepressant medication use, pain medication use, and motor vehicle collision in the past year were associated with positive ADHD screen.Conclusions.ADHD symptoms are associated with adverse medical and social outcomes that are in some cases gender specific.
Abstract 3658: 90K attenuates the development of colitis-associated colorectal tumors through negative regulation of intestinal epithelial Toll-like receptor 4
Authors: Chung Ik-Joo , Hwang Jun-Eul , Lee Ji-Hee
Published: May 2015

Background:

Inflammation is a risk factor for colon cancer. TLR4 expression is upregulated in almost all colitis-associated cancer and dysplasia. The molecular mechanisms linking inflammation and colon carcinogenesis are incompletely understood. We found that tumor associated antigen 90K antagonized TLR4/NF-kB signaling pathway in colon cancer cells. We hypothesized that 90K is involved in TLR4 expression in colitis-induced colon carcinogenesis.

Methods:

The effect of 90K against TLR4 was analysed by cell cultures, NF-kB reporter assay, and immunoprecipitation. C57BL/6 mice were treated with 3% dextran sodium sulfate (DSS) for acute colitis model. Colitis was assessed by disease activity index (DAI) including weight loss, stool consistency and rectal bleeding, and histopathology.

Results:

90K interacts with TLR4, suppresses the NF-kB signal in the MYD88-dependent pathway that is major adaptor molecules in TLRs/NF-kB signal. Adenoviral 90K administration attenuated the severity of acute DSS-induced colitis as assessed by DAI and histopathologic scoring compared with the control group. 90K restored body weight loss and the severity of acute DSS colitis through the suppression of TLR4/MYD88/NF-kB signal.

Conclusions:

Tumor associated antigen 90K is involved in negative regulation of inflammatory signaling triggered by TLR4-induced NF-kB activation. The data suggest that 90K might thereby suppress proliferation and progression of colon cancers.

Citation Format: Ik-Joo Chung, Jun-Eul Hwang, Ji-Hee Lee. 90K attenuates the development of colitis-associated colorectal tumors through negative regulation of intestinal epithelial Toll-like receptor 4. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3658. doi:10.1158/1538-7445.AM2014-3658