bluuebunny/crossref_metadata_2025 huggingface dataset:
Removal of Acetic Acid from Bacterial Culture Media by Adsorption onto a Two-Component Composite Polymer Gel
Authors: Kato Junya , Gotoh Takehiko , Nakashimada Yutaka
Published: March 2022

Organic acids, including acetic acid, are the metabolic products of many microorganisms. Acetic acid is a target product useful in the fermentation process. However, acetic acid has an inhibitory effect on microorganisms and limits fermentation. Thus, it would be beneficial to recover the acid from the culture medium. However, conventional recovery processes are expensive and environmentally unfriendly. Here, we report the use of a two-component hydrogel to adsorb dissociated and undissociated acetic acid from the culture medium. The Langmuir model revealed the maximum adsorption amount to be 44.8 mg acetic acid/g of dry gel at neutral pH value. The adsorption capacity was similar to that of an ion-exchange resin. In addition, the hydrogel maintained its adsorption capability in a culture medium comprising complex components, whereas the ion-exchange did not adsorb in this medium. The adsorbed acetic acid was readily desorbed using a solution containing a high salt concentration. Thus, the recovered acetic acid can be utilized for subsequent processes, and the gel-treated fermentation broth can be reused for the next round of fermentation. Use of this hydrogel may prove to be a more sustainable downstream process to recover biosynthesized acetic acid.
Self-Service System for the Family Members of ICU Patients: A Pilot Study
Authors: Chang I-Chiu , Hou Ying-Hui , Lu Li-Jung , Tung Yu-Chen
Published: March 2022

Family members of intensive care unit patients are often experience high anxiety and require more information about the patients. However, most Taiwanese healthcare institutions currently face manpower shortages due to the COVID-19 pandemic. Therefore, the task of providing additional services to meet family members’ needs and relieve their stress was deferred by some healthcare institutions. The self-service system, known to be effective and efficient in other industries, was recommended for use in the healthcare industry. This study aims to explore an intensive care unit self-service system (ICU-SSS) designed for the family members of ICU patients. This study investigates the feasibility of the system by following a mixed method approach, including qualitative interviews and a quantitative survey. Firstly, interviews with five family members and five ICU staff members of a case hospital were conducted to identify the need to develop an ICU-SSS for the family member. Secondly, a survey was completed by 30 family members to evaluate the system. The interview results reveal nine categories of family members’ needs and the survey results show that the ICU family members assigned acceptable scores to all the ICU-SSS functions, except the importance of “Logistical information”. Based on these findings, the scientific and practical implications are discussed.
5G-IPAKA: An Improved Primary Authentication and Key Agreement Protocol for 5G Networks
Authors: Xiao Yuelei , Wu Yang
Published: March 2022

The 3rd generation partnership project (3GPP) has been enhancing the security of the 5G AKA (authentication and key agreement) protocol. However, there may still be some shortcomings in the latest version of the 5G AKA protocol. According to the analysis of the latest version of the 5G AKA protocol, this paper points out seven of its shortcomings. To overcome these shortcomings, an improved primary authentication and key agreement protocol for 5G networks is proposed, which is named 5G-IPAKA. Compared with the latest version of the 5G AKA protocol, the main improvements include that the pre-shared key between the user equipment (UE) and the home network (HN) is replaced with a derivation key as the pre-shared key, the challenge-–response mechanism for the serving network (SN) is added, the mutual authentication and key confirmation occurs between the UE and the SN, and the message authentication code (MAC) failure procedure is replaced with a timeout mechanism on the HN. Then, the 5G-IPAKA protocol is proven secure in the mixed strand space model for mixed protocols. Further discussion and comparative analysis show that the 5G-IPAKA protocol can overcome the above shortcomings of the latest version of the 5G AKA protocol, and is better than the recently improved 5G AKA protocols. Additionally, the 5G-IPAKA protocol is efficient and backward-compatible.
Strategic Placement of Solar Power Plant and Interline Power Flow Controllers for Prevention of Blackouts
Authors: Mishra Akanksha , Venkata Nagesh Kumar Gundavarapu , Bali Sravana Kumar , Bathina Venkateswara Rao , Ramisetty Uma Maheswari , Gollapudi Srikanth , Habib Fayek Hady , Rusu Eugen
Published: March 2022

In these post COVID times, the world is going through a massive restructuring which India can use to its benefit by attracting foreign industrial investment. The major requirement is a reliable and ecofriendly electrical power source. Of late, renewable energy sources have increasingly become popular as alternative source of electricity. They can provide immense aid in improving the reliability of the power system, when placed properly. The alternative integrated energy sources along with FACTS devices can provide a promising future for reliable power systems. In this paper, an effective location for the solar power unit and Interline Power Flow Controller using Line Severity Index is proposed in order to avoid contingencies. An Indian 62 bus system and IEEE 57 bus system are considered for the study. The Firefly algorithm is used to tune the IPFC in the Integrated Energy Systems scenario, for a dual objective function. The effect of placement of the solar unit and the optimized IPFC is analyzed and studied in detail in this paper.
Social Relationships and the Health of Older Adults: An Examination of Social Connectedness and Perceived Social Support
Authors: Asante Samuel , Karikari Grace
Published: March 2022

This study examined the extent to which dimensions of social connectedness and perceived social support have distinct associations with the physical and mental health of older adults. This study utilized data from the Utah Fertility, Longevity, and Aging (FLAG) study. Participants included 259 older adults, aged 60 or older (mean age: 67.75 ± 4.8). Connectedness (networks and satisfaction with networks) was measured with the Duke Social Support Index. Social support (affective, confidant, instrumental) was measured with the Duke–UNC Functional Support Scale. Physical and mental health were measured with the Short Form Health Survey (SF-36). The results showed significant differences in the mean physical and mental health scores between participants with high scores on satisfaction with networks, affective, confidant, and instrumental support, and those with low scores on these dimensions. After controlling for covariates, affective support significantly predicted physical health, while satisfaction with networks, and affective and instrumental support significantly predicted mental health. The findings suggest that social support may be relatively more important to the health and wellbeing of older adults than social connectedness. This underscores the relative importance older adults attach to the quality rather than quantity of social ties.
Comparison of Surgical Outcomes between Trabeculectomy with Mitomycin C and Ahmed Valve Implantation with Mitomycin C in Eyes with Uveitic Glaucoma
Authors: Lee Seung Yeop , Kim Yong Hyun , Kim Ko Eun , Ahn Jaehong
Published: March 2022

We compared 1-year outcomes of trabeculectomy with mitomycin C (MMC) and Ahmed valve implantation with MMC as a first surgical procedure in patients with uveitic glaucoma. A total 38 eyes of 38 patients undergoing trabeculectomy (n =16) or Ahmed valve implantation (n = 22) were included. Surgical success was defined as intraocular pressure (IOP) ≤21 mmHg, IOP reduction ≥20% from baseline, no secondary glaucoma surgery, and no loss of light perception. The main outcome measurements including success rate, IOP, and the number of antiglaucoma medications and complications were compared. The overall success rates were comparable between the Ahmed and trabeculectomy groups (81.3 vs. 81.8%, p = 0.987). The mean IOPs were similar as well (p = 0.084), though the number of antiglaucoma medications was significantly lower in the trabeculectomy group than in the Ahmed group (1.0 ± 1.2 vs. 2.2 ± 1.1; p = 0.005). A statistically significant reduction in corneal endothelial cell density was noted in the Ahmed group (p = 0.004). Both treatments offered reasonable IOP control and safety for eyes with uveitic glaucoma. However, significantly fewer antiglaucoma medications were used in the trabeculectomy group. Furthermore, our results suggest that cautious postoperative monitoring with regard to corneal endothelial cell density should be additionally performed after Ahmed valve implantation.
Peri- and Interprosthetic Femoral Fractures—Current Concepts and New Developments for Internal Fixation
Authors: Kösters Clemens , den Toom Daniel , Metzlaff Sebastian , Daniilidis Kiriakos , Barz Linda , Roßlenbroich Steffen
Published: March 2022

Treatment of peri- and interprosthetic fractures represents a challenge in orthopedic trauma surgery. Multiple factors such as osteoporosis, polymedication and comorbidities impede therapy and the rehabilitation of this difficult fracture entity. This article summarizes current concepts and highlights new developments for the internal fixation of periprosthetic fractures. Since the elderly are unable to follow partial weight bearing, stable solutions are required. Therefore, a high primary stability is necessary. Numerous options, such as new angular stable plate systems with additional options for variable angle screw positioning, already exist and are in the process of being further improved. Lately, individually produced custom-made implants are offering interesting alternatives to treat periprosthetic fractures.
Therapeutic Effect of Intense Pulsed Light in Patients with Sjögren’s Syndrome Related Dry Eye
Authors: Huo Yanan , Wan Qi , Hou Xinzhu , Zhang Zhiyong , Zhao Jinchuan , Wu Zhiyi , Jin Xiuming
Published: March 2022

This prospective randomized study evaluated the efficacy and safety of intense pulsed light (IPL) and meibomian gland expression (MGX) as polytherapy for Sjögren’s Syndrome-related dry eye (SS-DE). The study enrolled 55 participants with SS-DE, 27 for the treatment group and 28 for the control group. The treatment group underwent three IPL-MGX treatments, three weeks apart. A randomly-selected eye from each patient was assessed at baseline and on weeks 9, 12, and 15 for Snellen best-corrected visual acuity (BCVA), intraocular pressure, Ocular Surface Disease Index (OSDI) score, conjunctival congestion, tear meniscus height, non-invasive tear breakup time (NBUT), Schirmer’s I test (SIT), corneal fluorescein staining (CFS), meibomian gland (MG) dropout, eyelid margin abnormality, MGX and meibum quality. OSDI, NBUT, CFS, MGX, and meibum quality were significantly improved in both groups, particularly in the treatment group. The eyelid margin abnormality improved significantly in the treatment but not in the control group on weeks 12 and 15. Snellen BCVA, conjunctival congestion, and SIT improved significantly in the treatment group, but the two groups were statistically similar. Our results indicated that three IPL-MGX sessions could significantly improve the subjective and objective characteristics of SS-DE, representing a promising treatment strategy.
Learning Curve Analysis of Single-Site Robot-Assisted Hysterectomy
Authors: Lee Yeon Jee , Lee Dong-Eun , Bae Jaekyung , Ha Hyeong In , Lim Myong Cheol
Published: March 2022

We aim to analyze the surgical outcomes and learning curve of single-site robot-assisted hysterectomy. This was a retrospective cohort study from a single academic medical center. A total of 123 patients who underwent single-site robotic surgery for gynecologic disease were enrolled. Gynecologic surgeries were performed by a single surgeon using single-site robot-assisted hysterectomy. The median age of enrolled patients was 49 years (range: 30–74 years). The median operation time was 131 min (range: 59–502 min) and the median docking time was 3 min (range: 1–10 min). In addition, the median console time was 76 min (range: 29–465 min). The cumulative sum (CUSUM) graph for total operation time indicated an initial decrease at case 41, generating 3 distinct performance phases: learning (n = 41 initial cases), competence (n = 54 middle cases), and mastery (n = 28 final cases). There was one case conversion to open surgery due to the difficulty in securing the field of view because of a 16-cm bulky mass protruding from the left pelvic wall. No patients required a transfusion and two complications including vaginal cuff dehiscence were identified. The single-site robot-assisted hysterectomy is a safe and feasible procedure. The learning curve consisted of 41 cases to significantly decrease the total operation time.
Influence of Line Processing Parameters on Properties of Carbon Fibre Epoxy Towpreg
Authors: Çelik Murat , Noble Thomas , Jorge Frank , Jian Rongqing , Ó Brádaigh Conchúr M. , Robert Colin
Published: March 2022

This paper explores the performance of low-cost unidirectional carbon fibre towpregs with respect to line production speed and fibre volume fraction. Using an automated production line, towpregs were produced at different production speeds, resulting in modified fibre volume fractions. The towpregs were used to manufacture unidirectional composite plates, which were then tested to evaluate mechanical performance. The fibre straightness and interfacial void ratio of the composite plates were determined by statistical analysis of the samples’ optical micrographs. The results demonstrate that adjusting the line production speed enables targeted fibre volume fractions (FVF) to be reached, resulting in the composites having different mechanical performances (2039 MPa and 2186.7 MPa tensile strength, 1.26 and 1.21 GPa flexural strength for 59.8% and 64.4% FVF, respectively). It was shown that at lower production speeds and FVF, composites exhibit good consolidation and low porosity, which is highlighted by the better interlaminar shear strength performances (8.95% increase), indicating the limitations of manufacturing very high FVF composites. Furthermore, it was concluded that fibre straightness plays a key role in mechanical performance, as samples with a lesser degree of fibre straightness showed a divergence from theoretical tensile properties.
Silicon-Compatible Memristive Devices Tailored by Laser and Thermal Treatments
Authors: Koryazhkina Maria N. , Filatov Dmitry O. , Tikhov Stanislav V. , Belov Alexey I. , Korolev Dmitry S. , Kruglov Alexander V. , Kryukov Ruslan N. , Zubkov Sergey Yu. , Vorontsov Vladislav A. , Pavlov Dmitry A. , Tetelbaum David I. , Mikhaylov Alexey N. , Shchanikov Sergey A. , Kim Sungjun , Spagnolo Bernardo
Published: March 2022

Nowadays, memristors are of considerable interest to researchers and engineers due to the promise they hold for the creation of power-efficient memristor-based information or computing systems. In particular, this refers to memristive devices based on the resistive switching phenomenon, which in most cases are fabricated in the form of metal–insulator–metal structures. At the same time, the demand for compatibility with the standard fabrication process of complementary metal–oxide semiconductors makes it relevant from a practical point of view to fabricate memristive devices directly on a silicon or SOI (silicon on insulator) substrate. Here we have investigated the electrical characteristics and resistive switching of SiOx- and SiNx-based memristors fabricated on SOI substrates and subjected to additional laser treatment and thermal treatment. The investigated memristors do not require electroforming and demonstrate a synaptic type of resistive switching. It is found that the parameters of resistive switching of SiOx- and SiNx-based memristors on SOI substrates are remarkably improved. In particular, the laser treatment gives rise to a significant increase in the hysteresis loop in I–V curves of SiNx-based memristors. Moreover, for SiOx-based memristors, the thermal treatment used after the laser treatment produces a notable decrease in the resistive switching voltage.
Comparative Assessment and Parametric Optimisation of Large Marine Two-Stroke Engines with Exhaust Gas Recirculation and Alternative Turbocharging Systems
Authors: Lu Daoyi , Theotokatos Gerasimos , Zhang Jundong , Zeng Hong , Cui Keying
Published: March 2022

Although the exhaust gas recirculation (EGR) technology has been proven effective to decrease the marine engine’s nitrogen oxides (NOx) emissions, it is associated with a considerable fuel consumption increase and challenges to the engine–turbocharger matching. This study aims to parametrically optimise the EGR and turbocharging system settings of a large marine two-stroke engine with the objective of obtaining the highest engine efficiency whilst ensuring compliance with the prevailing NOx emissions limits. Two typical configurations of the investigated engine (baseline and alternative) are modelled in the GT-SUITE software. Parametric simulations are performed with EGR rates up to 40% along with cylinder bypass rates up to 50%, and the simulation results are analysed to quantify the impact of the engine operation with EGR on the performance and NOx emissions parameters. For the baseline engine configuration, the EGR rate increase considerably deteriorates the brake specific fuel consumption (BSFC), which is attenuated by opening the cylinder bypass valve. The optimal combinations of the EGR and cylinder bypass rates for each operating point are identified for both configurations. Following the comparative assessment between the two engine configurations, recommendations for the engine operating modes are proposed, leading to BSFC improvement in the region of 0.7 to 2.9 g/kWh. This study provides insights for the operational settings optimisation of two-stroke engines equipped with EGR systems, contributing towards the reduction of the associated environmental carbon footprint.
Gatekeepers as Safekeepers—Mapping Audiences’ Attitudes towards News Media’s Editorial Oversight Functions during the COVID-19 Crisis
Authors: Olsen Ragnhild Kristine , Solvoll Mona Kristin , Futsæter Knut-Arne
Published: March 2022

This study investigates people’s attitudes towards news media’s role as gatekeepers during the coronavirus pandemic. Specifically, this concerns news media’s quality control and the selection of the most important news about the pandemic, as well as the provision of useful information and knowledge about the virus and its implications. Challenging research that has questioned the very idea of journalistic gatekeeping in hybrid media systems, we set out to explore people’s attitudes towards news media’s gatekeeper functions during a crisis, when the need for reliable and relevant information is extraordinarily high and the information environment is flooded with disinformation. In this situation, news media gatekeepers could serve as safekeepers that protect the population. Based on a national survey in Norway (N = 1024), a country characterized by high levels of trust in social institutions, including the national press, the study finds that people were generally supportive of news media’s gatekeeper functions amid the pandemic. However, there were noteworthy demographic differences. Older people, women, and those who were more highly educated showed more positive attitudes towards news media’s gatekeeping. Moreover, we found lower support for news media’s gatekeeping in the group who trusted alternative, right-wing news media.
A Personalized Approach to Treat Advanced Stage Severely Contracted Joints in Dupuytren’s Disease with a Unique Skeletal Distraction Device—Utilizing Modern Imaging Tools to Enhance Safety for the Patient
Authors: Müller-Seubert Wibke , Cai Aijia , Arkudas Andreas , Ludolph Ingo , Fritz Niklas , Horch Raymund E.
Published: March 2022

Background: While surgical therapy for Dupuytren’s disease is a well-established standard procedure, severe joint flexion deformities in advanced Dupuytren’s disease remain challenging to treat. Skeletal distraction has proven to be an additional treatment option. Methods: We analyzed the surgical treatment algorithm, including the application of a skeletal distraction device, in patients with a flexion deformity due to Dupuytren’s disease, Iselin stage III or IV, who were operated on from 2003 to 2020 in our department. Results: From a total of 724 patients, we included the outcome of 55 patients’ fingers in this study, who had undergone additional skeletal joint distraction with our Erlangen device. Additional fasciotomy or fasciectomy, in a one- or two-staged procedure, was performed in all patients, according to the individual findings and necessities. The range of motion of the PIP joint improved from 12° to 53°. A number of complications, in all steps of the treatment, were noted in a total of 36.4% of patients, including the development of fractures (16.4%), followed by vessel injury, pin infections, and complex regional pain syndrome (5%). Conclusions: Additional skeletal distraction improves the range of motion of severely contracted joints in Dupuytren’s disease. Nevertheless, careful patient selection is necessary, due to the moderate rate of complications.
Incorporating Biomarkers in COPD Management: The Research Keeps Going
Authors: Pantazopoulos Ioannis , Magounaki Kalliopi , Kotsiou Ourania , Rouka Erasmia , Perlikos Fotis , Kakavas Sotirios , Gourgoulianis Konstantinos
Published: March 2022

Globally, chronic obstructive pulmonary disease (COPD) remains a major cause of morbidity and mortality, having a significant socioeconomic effect. Several molecular mechanisms have been related to COPD including chronic inflammation, telomere shortening, and epigenetic modifications. Nowadays, there is an increasing need for novel therapeutic approaches for the management of COPD. These treatment strategies should be based on finding the source of acute exacerbation of COPD episodes and estimating the patient’s own risk. The use of biomarkers and the measurement of their levels in conjunction with COPD exacerbation risk and disease prognosis is considered an encouraging approach. Many types of COPD biomarkers have been identified which include blood protein biomarkers, cellular biomarkers, and protease enzymes. They have been isolated from different sources including peripheral blood, sputum, bronchoalveolar fluid, exhaled air, and genetic material. However, there is still not an exclusive biomarker that is used for the evaluation of COPD but rather a combination of them, and this is attributed to disease complexity. In this review, we summarize the clinical significance of COPD-related biomarkers, their association with disease outcomes, and COPD patients’ management. Finally, we depict the various samples that are used for identifying and measuring these biomarkers.
Clinical Approaches to Late-Onset Psychosis
Authors: Kim Kiwon , Jeon Hong Jin , Myung Woojae , Suh Seung Wan , Seong Su Jeong , Hwang Jae Yeon , Ryu Je il , Park Seon-Cheol
Published: March 2022

Psychosis can include schizophrenia, mood disorders with psychotic features, delusional disorder, active delirium, and neurodegenerative disorders accompanied by various psychotic symptoms. Late-onset psychosis requires careful intervention due to the greater associated risks of secondary psychosis; higher morbidity and mortality rates than early-onset psychosis; and complicated treatment considerations due to the higher incidence of adverse effects, even with the black box warning against antipsychotics. Pharmacological treatment, including antipsychotics, should be carefully initiated with the lowest dosage for short-term efficacy and monitoring of adverse side effects. Further research involving larger samples, more trials with different countries working in consortia, and unified operational definitions for diagnosis will help elaborate the clinical characteristics of late-onset psychosis and lead to the development of treatment approaches.
A New Earth Observation Service Based on Sentinel-1 and Sentinel-2 Time Series for the Monitoring of Redevelopment Sites in Wallonia, Belgium
Authors: Petit Sophie , Stasolla Mattia , Wyard Coraline , Swinnen Gérard , Neyt Xavier , Hallot Eric
Published: March 2022

Urban planning is a challenge, especially when it comes to limiting land take. In former industrial regions such as Wallonia, the presence of a large number of brownfields, here called “redevelopment sites”, opens up new opportunities for sustainable urban planning through their revalorization. The Walloon authorities are currently managing an inventory of more than 2200 sites, which requires a significant amount of time and resources to update. In this context, the Sentinel satellites and the Terrascope platform, the Sentinel Collaborative Ground Segment for Belgium, enabled us to deploy SARSAR, an Earth observation service used for the automated monitoring of redevelopment sites that generates regular and automatic change reports that are directly usable by the Walloon authorities. In this paper, we present the methodological aspects and implementation details of the service, which combines two well-known and robust methods: the Pruned Exact Linear Time method for change point detection and threshold-based classification, which assigns the detected changes to three different classes (vegetation, building and soil). The overall accuracy of the system is in the range of 70–90%, depending on the different methods and classes considered. Some remarks on the advantages and possible drawbacks of this approach are also provided.
Improving the Representation of Climate Change Adaptation Behaviour in New Zealand’s Forest Growing Sector
Authors: Villamor Grace B. , Dunningham Andrew , Stahlmann-Brown Philip , Clinton Peter W.
Published: March 2022

To provide the forest industry with a better understanding of alternatives to simulate future adaptation pathways under evolving climatic and socio-economic uncertainty, we review the literature on how adaptation decisions are modelled in the context of plantation forests. This review leads to the conclusion that the representation of adaptation behaviour and decision-making remain very limited in most of the agent-based models in the forestry sector. Moreover, theoretical frameworks used to understand the adaptation behaviour of forest owners are also lacking. In this paper, we propose the application of protection motivation theory (PMT) as a framework to understand the motivation of forest owners to reduce the negative impacts of climate change on their forest plantations. Furthermore, the use of PMT allows factors affecting the maladaptive behaviour of forest owners to be examined. A survey of New Zealand foresters showed that less than 10% of smallholder forest owners adopted adaptation strategies. This result highlights the importance of addressing the research question “what motivates forest owners to take risk reduction measures?” Exploring this question is crucial to the future success of the New Zealand forestry sector and we suggest that it can be addressed by using PMT. This paper proposes a conceptual framework for an agent-based model as an alternative to simulating adaptation pathways for forest plantations in New Zealand.
Revisiting the Copula-Based Trading Method Using the Laplace Marginal Distribution Function
Authors: Nadaf Tayyebeh , Lotfi Taher , Shateyi Stanford
Published: March 2022

Pairs trading under the copula approach is revisited in this paper. It is well known that financial returns arising from indices in markets may not follow the features of normal distribution and may exhibit asymmetry or fatter tails, in particular. Due to this, the Laplace distribution is employed in this work to fit the marginal distribution function, which will then be employed in a copula function. In fact, a multivariate copula function is constructed on two indices (based on the Laplace marginal distribution), enabling us to obtain the associated probabilities required for the process of pairs trade and creating an efficient tool for trading.
Natural Gas Scarcity Risk in the Belt and Road Economies Based on Complex Network and Multi-Regional Input-Output Analysis
Authors: Du Ruijin , Wu Qi , Nan Ziwei , Dong Gaogao , Tian Lixin , Wu Feifan
Published: March 2022

Natural gas scarcity poses a significant risk to the global economy. The risk of production loss due to natural gas scarcity can be transferred to downstream economies through globalized supply chains. Therefore, it is important to quantify and analyze how natural gas scarcity in some regions affects the Belt and Road (B&R) economies. The embodied natural gas scarcity risks (EGSRs) of B&R economies are assessed and the EGSR transmission network is constructed. The built network shows a small-world nature. This illustrates that any interruption in key countries will quickly spread to neighboring countries, potentially affecting the global economy. The top countries, including Turkey, China, Ukraine, and India are identified in EGSR exports, which also have relatively high values of closeness centrality. The findings illustrate that the shortage of natural gas supply in these countries may have a significant impact on downstream countries or sectors and the resulting economic losses spread rapidly. These countries are critical to the resilience of the B&R economies to natural gas scarcity. The top nations, including Turkmenistan, Macedonia, and Georgia are also identified in EGSR imports, highlighting their vulnerability to natural gas scarcity. Further, the community analysis of the network provides a fresh perspective for formulating fair and reasonable allocation policies of natural gas resources and minimizing the large-scale spread of economic losses caused by natural gas scarcity.
Cyanobacteria Secondary Metabolites as Biotechnological Ingredients in Natural Anti-Aging Cosmetics: Potential to Overcome Hyperpigmentation, Loss of Skin Density and UV Radiation-Deleterious Effects
Authors: Favas Rita , Morone Janaína , Martins Rosário , Vasconcelos Vitor , Lopes Graciliana
Published: March 2022

The loss of density and elasticity, the appearance of wrinkles and hyperpigmentation are among the first noticeable signs of skin aging. Beyond UV radiation and oxidative stress, matrix metalloproteinases (MMPs) assume a preponderant role in the process, since their deregulation results in the degradation of most extracellular matrix components. In this survey, four cyanobacteria strains were explored for their capacity to produce secondary metabolites with biotechnological potential for use in anti-aging formulations. Leptolyngbya boryana LEGE 15486 and Cephalothrix lacustris LEGE 15493 from freshwater ecosystems, and Leptolyngbya cf. ectocarpi LEGE 11479 and Nodosilinea nodulosa LEGE 06104 from marine habitats were sequentially extracted with acetone and water, and extracts were analyzed for their toxicity in cell lines with key roles in the skin context (HaCAT, 3T3L1, and hCMEC). The non-toxic extracts were chemically characterized in terms of proteins, carotenoids, phenols, and chlorophyll a, and their anti-aging potential was explored through their ability to scavenge the physiological free radical superoxide anion radical (O2•−), to reduce the activity of the MMPs elastase and hyaluronidase, to inhibit tyrosinase and thus avoid melanin production, and to block UV-B radiation (sun protection factor, SPF). Leptolyngbya species stood out for anti-aging purposes: L. boryana LEGE 15486 presented a remarkable SPF of 19 (at 200 µg/mL), being among the best species regarding O2•− scavenging, (IC50 = 99.50 µg/mL) and also being able to inhibit tyrosinase (IC25 = 784 µg/mL), proving to be promising against UV-induced skin-aging; L. ectocarpi LEGE 11479 was more efficient in inhibiting MMPs (hyaluronidase, IC50 = 863 µg/mL; elastase, IC50 = 391 µg/mL), thus being the choice to retard dermal density loss. Principal component analysis (PCA) of the data allowed the grouping of extracts into three groups, according to their chemical composition; the correlation of carotenoids and chlorophyll a with MMPs activity (p < 0.01), O2•− scavenging with phenolic compounds (p < 0.01), and phycocyanin and allophycocyanin with SPF, pointing to these compounds in particular as responsible for UV-B blockage. This original survey explores, for the first time, the biotechnological potential of these cyanobacteria strains in the field of skin aging, demonstrating the promising, innovative, and multifactorial nature of these microorganisms.
The Feasibility of a Machine Learning Approach in Predicting Successful Ventilator Mode Shifting for Adult Patients in the Medical Intensive Care Unit
Authors: Cheng Kuang-Hua , Tan Mei-Chu , Chang Yu-Jen , Lin Cheng-Wei , Lin Yi-Han , Chang Tzu-Min , Kuo Li-Kuo
Published: March 2022

Background and Objectives: Traditional assessment of the readiness for the weaning from the mechanical ventilator (MV) needs respiratory parameters in a spontaneous breath. Exempted from the MV disconnecting and manual measurements of weaning parameters, a prediction model based on parameters from MV and electronic medical records (EMRs) may help the assessment before spontaneous breath trials. The study aimed to develop prediction models using machine learning techniques with parameters from the ventilator and EMRs for predicting successful ventilator mode shifting in the medical intensive care unit. Materials and Methods: A retrospective analysis of 1483 adult patients with mechanical ventilators for acute respiratory failure in three medical intensive care units between April 2015 and October 2017 was conducted by machine learning techniques to establish the predicting models. The input candidate parameters included ventilator setting and measurements, patients’ demographics, arterial blood gas, laboratory results, and vital signs. Several classification algorithms were evaluated to fit the models, including Lasso Regression, Ridge Regression, Elastic Net, Random Forest, Extreme Gradient Boosting (XGBoost), Support Vector Machine, and Artificial Neural Network according to the area under the Receiver Operating Characteristic curves (AUROC). Results: Two models were built to predict the success shifting from full to partial support ventilation (WPMV model) or from partial support to the T-piece trial (sSBT model). In total, 3 MV and 13 nonpulmonary features were selected for the WPMV model with the XGBoost algorithm. The sSBT model was built with 8 MV and 4 nonpulmonary features with the Random Forest algorithm. The AUROC of the WPMV model and sSBT model were 0.76 and 0.79, respectively. Conclusions: The weaning predictions using machine learning and parameters from MV and EMRs have acceptable performance. Without manual measurements, a decision-making system would be feasible for the continuous prediction of mode shifting when the novel models process real-time data from MV and EMRs.
Atrial Fibrillation and Dementia: Epidemiological Insights on an Undervalued Association
Authors: Saglietto Andrea , Ballatore Andrea , Xhakupi Henri , De Ferrari Gaetano Maria , Anselmino Matteo
Published: March 2022

Background and objectives: Atrial fibrillation (AF) and dementia are growing causes of morbidity and mortality, representing relevant medical and socioeconomic burdens. In this study, based on data from the Global Burden of Disease Injuries and Risk Factors Study (GBD) 2019, we focused on AF and dementia distribution and investigated the potential correlation between the two epidemiological trends. Materials and Methods: Crude and age-standardized incidence, prevalence, mortality rate, and disability-adjusted life years (DALYs) lost, derived from GBD 2019, were reported for AF and dementia. Global features were also stratified by high and low sociodemographic-index (SDI) countries. Granger test analysis was performed to investigate the correlation between AF and dementia incidence time trends. Results: From 1990 to 2019 crude worldwide incidence and prevalence showed a dramatic increase for both conditions (from 43.24 to 61.01 and from 528.72 to 771.51 per 100,000 individuals for AF, respectively; from 54.60 to 93.52 and from 369.88 to 667.2 per 100,000 individuals for dementia, respectively). In the same timeframe, crude mortality rate doubled for AF and dementia (from 2.19 to 4.08, and from 10.49 to 20.98 per 100,000 individuals, respectively). Age-standardized estimate showed a substantial stability over the years, highlighting the key role of the progressively aging population. Crude estimates of all of the investigated metrics are greater in high SDI countries for both conditions. This association was still valid for age-standardized metrics, albeit by a reduced magnitude, suggesting the presence of higher risk factor burden in these countries. Finally, according to Granger test, we found a significant association between the historical trends of AF and dementia incidence (p = 0.004). Conclusions: AF and dementia burden progressively increased in the last three decades. Given the potential association between these two conditions, further clinical data assessing this relationship is needed.
US Cancer Screening Recommendations: Developments and the Impact of COVID-19
Authors: Barsouk Adam , Saginala Kalyan , Aluru John Sukumar , Rawla Prashanth , Barsouk Alexander
Published: March 2022

The USPSTF and ACS recommend screening for breast, cervical, colorectal, and lung cancers. Rates of cancer screening, diagnosis, and treatment decreased significantly in the US and other developed nations during the height of the COVID-19 pandemic and lockdown (April 2020) and have since recovered, although not to baseline levels in many cases. For breast cancer, the USPSTF recommends biennial screening with mammography for women aged 50–74, while the ACS recommends annual screening for women aged 45–54, who may transition to biennial after 55. Minority and rural populations have lower rates of screening and lower utilization of DBT, which offers superior sensitivity and specificity. Among 20 US health networks in April 2020, mammography rates were down 89.2% and new breast cancer diagnoses down by 50.5%. For cervical cancer, the USPSTF recommends cervical cytology every three years for women 21–65, or cytology+hrHPV co-testing every five years for women aged 30–65. Cervical cancer screening rates declined by 87% in April 2020 and recovered to a 40% decline by June 2020, with American Indians and Asians most severely affected. For colorectal cancer (CRC), the USPSTF and ACS recommend screening for ages 45–75, recently lowered from a starting age of 50. Most commonly-used modalities include annual FIT testing, FIT+DNA testing every three years, and colonoscopy every ten years, with shorter repeat if polyps are found. In the US, CRC screenings were down by 79–84.5% in April 2020 across several retrospective studies. Patient encounters for CRC were down by 39.9%, and a UK-based model predicted that 5-year-survival would decrease by 6.4%. The USPSTF recommends screening low dose CT scans (LDCTs) for ages 50–80 with a >20 pack-year smoking history who have smoked within the past 15 years. In April 2020, screening LDCTs fell by 72–78% at one US institution and lung cancer diagnoses were down 39.1%.
Predicting the Strength of EBAM 3D Printed Ti-6Al-4V from Processing Conditions
Authors: Johnson Tanya , Peters Abbey , Harlow D. Gary , Haden Christina Viau
Published: March 2022

In this study, a process-to-property linear regression model was developed to predict the yield and ultimate tensile strengths of as printed Ti-6Al-4V from electron beam additive manufacturing (EBAM). A total of 8 printing conditions such as bead width, wire feed rate, deposition speed were utilized to predict the material properties in three different notional parts produced over a period of several months. It was found that as the precision and variety of processing conditions collected during print improved between prints, so did the predictive ability of the model. In the final print, the model predicted the yield and ultimate strengths of 72 specimens with an R2 correlation of 0.8 and 0.6 for the horizontal and vertical test specimens, respectively. Although the current model indirectly accounted for thermal fluctuations, further improvements to the model’s ability to predict material strength are expected with the addition of thermal data captured in subsequent notional parts.
Influence of Deposition Plane Angle and Saline Corrosion on Fatigue Crack Growth in Maraging Steel Components Produced by Laser Powder Bed Fusion
Authors: Fernandes Rui F. , de Jesus Joel , Borrego Luís , Vilhena Luís , Ramalho Amílcar , Ferreira José A. M.
Published: March 2022

Maraging steels are used in several industries, namely in the molds industry. The determination of fatigue crack propagation resistance in 18Ni300 maraging steel at the Paris regime is a vital issue for safety-relevant components, which are designed to work for a large number of loading cycles before periodic inspections. The main goal of this work is to analyze the influence of the deposition plane angle and saline corrosion on fatigue crack growth in maraging steel samples produced by Laser Powder Bed Fusion (LPBF). The crack closure parameter was used in order to analyze the different fatigue crack growth behaviors, as well as the metallographic, hardness, fractography and corrosion/oxidation analysis. From this work, the main achievement was that the deposition plane angle did not reveal a notable influence in the fatigue crack growth behavior for the fatigue tests unsubmitted to saline corrosion. On the other hand, the fatigue crack growth behavior for the tests under saline corrosion showed an increase in the crack closure parameter due to the appearance of the crack closure induced by oxides, which reduced the fatigue crack growth speed. This phenomenon depends on the deposition plane angle, which controls the martensite amount and consequently controls the level of corrosion/oxidation.
Changes in Plasma Lipid Levels Following Cortical Spreading Depolarization in a Transgenic Mouse Model of Familial Hemiplegic Migraine
Authors: Loonen Inge C. M. , Kohler Isabelle , Ghorasaini Mohan , Giera Martin , van den Maagdenberg Arn M. J. M. , Mayboroda Oleg A. , Tolner Else A.
Published: March 2022

Metabolite levels in peripheral body fluids can correlate with attack features in migraine patients, which underscores the potential of plasma metabolites as possible disease biomarkers. Migraine headache can be preceded by an aura that is caused by cortical spreading depolarization (CSD), a transient wave of neuroglial depolarization. We previously identified plasma amino acid changes after CSD in familial hemiplegic migraine type 1 (FHM1) mutant mice that exhibit increased neuronal excitability and various migraine-related features. Here, we aimed to uncover lipid metabolic pathways affected by CSD, guided by findings on the involvement of lipids in hemiplegic migraine pathophysiology. Using targeted lipidomic analysis, we studied plasma lipid metabolite levels at different time points after CSD in wild-type and FHM1 mutant mice. Following CSD, the most prominent plasma lipid change concerned a transient increase in PGD2, which lasted longer in mutant mice. In wild-type mice only, levels of anti-inflammatory lipid mediators DPAn-3, EPA, ALA, and DHA were elevated 24 h following CSD compared to Sham-treated animals. Given the role of PGs and neuroinflammation in migraine pathophysiology, our findings underscore the potential of monitoring peripheral changes in lipids to gain insight in central brain mechanisms.
8-oxoguanine and 8-oxodeoxyguanosine Biomarkers of Oxidative DNA Damage: A Review on HPLC–ECD Determination
Authors: Chiorcea-Paquim Ana-Maria
Published: March 2022

Reactive oxygen species (ROS) are continuously produced in living cells due to metabolic and biochemical reactions and due to exposure to physical, chemical and biological agents. Excessive ROS cause oxidative stress and lead to oxidative DNA damage. Within ROS-mediated DNA lesions, 8-oxoguanine (8-oxoG) and its nucleotide 8-oxo-2′-deoxyguanosine (8-oxodG)—the guanine and deoxyguanosine oxidation products, respectively, are regarded as the most significant biomarkers for oxidative DNA damage. The quantification of 8-oxoG and 8-oxodG in urine, blood, tissue and saliva is essential, being employed to determine the overall effects of oxidative stress and to assess the risk, diagnose, and evaluate the treatment of autoimmune, inflammatory, neurodegenerative and cardiovascular diseases, diabetes, cancer and other age-related diseases. High-performance liquid chromatography with electrochemical detection (HPLC–ECD) is largely employed for 8-oxoG and 8-oxodG determination in biological samples due to its high selectivity and sensitivity, down to the femtomolar range. This review seeks to provide an exhaustive analysis of the most recent reports on the HPLC–ECD determination of 8-oxoG and 8-oxodG in cellular DNA and body fluids, which is relevant for health research.
The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes
Authors: Alenezi Samya S. , Alenezi Naif D. , Ebiloma Godwin U. , Natto Manal J. , Ungogo Marzuq A. , Igoli John O. , Ferro Valerie A. , Gray Alexander I. , Fearnley James , de Koning Harry P. , Watson David G.
Published: March 2022

Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.