bluuebunny/crossref_metadata_2025 huggingface dataset:
Conceptual Study and Development of an Autonomously Operating, Sailing Renewable Energy Conversion System
Authors: Rickert Christopher , Thevar Parambil Anurag , Leimeister Mareike
Published: June 2022

With little time left for humanity to reduce climate change to a tolerable level, a highly scalable and rapidly deployable solution is needed that can be implemented by any country. Offshore wind energy in international waters is an underused resource and could even be harnessed by landlocked countries. In this paper, the use of sailing wind turbines operating autonomously in high seas to harvest energy is proposed. The electrical energy that is generated by the wind turbine is converted to a renewable fuel and stored onboard. Later, the fuel will be transferred to shore or to other destinations of use. The presented idea is explored at the system level, where the basic subsystems necessary are identified and defined, such as energy conversion and storage as well as propulsion subsystems. Moreover, various operating possibilities are investigated, including a comparison of different sailing strategies and fuels for storage. Existing ideas are also briefly addressed and an example concept is suggested as well. In this paper, the proposed sailing renewable energy conversion system is explored at a higher level of abstraction. Following up on this conceptual study, more detailed investigations are necessary to determine whether the development of such a sailing renewable energy conversion system is viable from an engineering, economic, and environmental point of view.
Regularly Planted Rather Than Natural Understory of Norway Spruce (Picea abies H. Karst.) Contributes to the Individual Stability of Canopy Silver Birch (Betula pendula Roth.)
Authors: Krišāns Oskars , Matisons Roberts , Vuguls Jānis , Bāders Endijs , Rust Steffen , Elferts Didzis , Saleniece Renāte , Jansons Āris
Published: June 2022

Forest plantations, particularly high-density planted stands, are considered to be more prone to wind damage compared to naturally regenerated stands. The wind resistance (mechanical stability) of plantations can, however, be improved by close-to-natural management, for example, combining pioneer and shade-tolerant species. Presumably, the stability of such stands would be enhanced by the reduced competition of canopy trees and stronger root contacts provided by understory trees, which depend on spatial distribution. In the hemiboreal forest zone, silver birch (Betula pendula Roth.) and Norway spruce (Picea abies (L.) H.Karst.) form such a combination naturally. In this study, the static tree-pulling tests were performed to estimate the mechanical stability of canopy silver birch growing with random Norway spruce understory in naturally regenerated (post-clear-cut) and regularly planted bi-species mixed stands. The regular mixing of the high-density bi-species stand significantly improved the loading resistance of canopy silver birch compared to the naturally regenerated stands of similar composition and age. Such an effect might be related to the stratification of the canopy space between pioneer birch and shade-tolerant spruce, which improved the individual stability of the canopy trees. Further, a regular rooting network of the planted stands likely contributed to the stability by reducing weak spots. Accordingly, the wind resistance of trees in regularly planted bi-species stands might be improved, avoiding additional management.
Isolation of Novel Yeast from Coconut (Cocos nucifera L.) Water and Phenotypic Examination as the Potential Parameters in Bioethanol Production
Authors: Kasmiarti Getari , Oktiarni Dwita , Hariani Poedji Loekitowati , Novia Novia , Hermansyah Hermansyah
Published: June 2022

Yeast is a fermentation agent for producing bioethanol as an environmentally friendly alternative energy. Therefore, this study aims to find novel yeasts with the capability to persevere under acidic, high temperature, and high sugar content conditions, which are required in the bioethanol industry. The yeasts were isolated and identified from coconut (Cocos nucifera L.) water by a DNA sequencing method and phenotypic test. Yeast isolation has been completed with a serial dilution procedure and purification was conducted with HiPurA Genomic DNA Purification Spin Kits, which were analyzed by DNA Sequencing. The phenotypic test was carried out with thermotolerant (30 °C and 41 °C), high acidity (lactic acid), and sugar content (molasses 35 °brix) parameters in the media as the initial step of yeast ability screening. Based on the results, the three species of Candida tropicalis K5 (Candida tropicalis strain L2), K15 (Candida tropicalis strain MYA-3404), and K20 (Candida tropicalis strain Y277) obtained met the phenotypic standards. This showed that the yeasts have the potential to produce molasses-based bioethanol.
Valorization of Low-Cost Substrates for the Production of Odd Chain Fatty Acids by the Oleaginous Yeast Yarrowia lipolytica
Authors: El Kantar Sally , Koubaa Mohamed
Published: June 2022

Odd-chain fatty acids (OCFAs) have recently gained interest as target compounds in microbial production due to their diverse applications in the medical, pharmaceutical and chemical industries for the production of biofuels. Yarrowia lipolytica is a promising oleaginous yeast that has the ability to accumulate high quantities of fatty acids. However, the use of Y. lipolytica oils is still under research, in order to decrease the production costs related to the fermentation process and improve economic feasibility. In this work, sugar beet molasses (10–50 g/L) and crude glycerol (30 g/L) were used as the main carbon sources to reduce the processing costs of oil production from a genetically engineered Y. lipolytica strain. The effects of medium composition were studied on biomass production, lipid content, and OCFAs profile. Lipid production by yeast growing on molasses (20 g/L sucrose) and crude glycerol reached 4.63 ± 0.95 g/L of culture medium. OCFAs content represented 58% of the total fatty acids in lipids, which corresponds to ≈2.69 ± 0.03 g/L of culture medium. The fermentation was upscaled to 5 L bioreactors and fed-batch co-feeding increased OCFA accumulation in Y. lipolytica by 56% compared to batch cultures.
Genotoxicity Comparison between Morinda citrifolia Fruit and Seed Substances
Authors: Shin Sarah , Kim Ji Soo , Park Myung Ku , Bang Ok-Sun
Published: June 2022

This study aimed to evaluate the genotoxic potential of the fruit and seed powder, fruit puree, and aqueous extracts of Morinda citrifolia (Rubiaceae, noni). The genotoxic potential of the noni substances was evaluated using in vitro Ames, in vitro chromosomal aberration, and in vivo micronucleus tests. All test procedures were conducted per Organization for Economic Cooperation and Development guidelines, and good laboratory practice. None of the noni fruit test substances showed genotoxic signs up to 5000 and 2000 μg/plate in the Ames and micronucleus tests, respectively. In the chromosomal aberration test, neither the fruit puree nor aqueous extract showed structural and numerical aberrations up to 5000 and 4650 μg/mL, respectively, irrespective of metabolic activation, in both 6 h and 24 h treatment groups. The safe ranges of noni fruit and seed powders were up to 2500 and 2100 μg/mL, respectively, in the 6 h treatment group and up to 1600–4100 and 370–450 μg/mL, respectively, in both 6 h and 24 h treatment groups in the presence of metabolic activation. Noni fruit and seeds were safe in terms of genotoxicity under our experimental conditions. Our data are the first to provide valuable genotoxic information on Morinda citrifolia.
Optimization of Ultrasonic-Assisted Bioactive Compound Extraction from Green Soybean (Glycine max L.) and the Effect of Drying Methods and Storage Conditions on Procyanidin Extract
Authors: Khonchaisri Rattanaporn , Sumonsiri Nutsuda , Prommajak Trakul , Rachtanapun Pornchai , Leksawasdi Noppol , Techapun Charin , Taesuwan Siraphat , Halee Anek , Nunta Rojarej , Khemacheewakul Julaluk
Published: June 2022

Green soybean (Glycine max L.) seeds (GSS) are rich in various antioxidants and phytonutrients that are linked to various health benefits. Ultrasound-assisted extraction (UAE) technology was used for extracting the effective components from GSS. A response surface method (RSM) was used to examine the influence of liquid-to-solid ratio and extraction temperature on the bioactive compounds and antioxidant characteristics. The optimal conditions were a liquid-to-solid ratio of 25:1 and a UAE temperature of 40 °C. The observed values coincided well with the predicted values under optimal conditions. Additionally, the effects of drying methods on the procyanidins and antioxidant activities of GSS extract were evaluated. The spray-dried GSS extract contained the highest levels of procyanidins (21.4 ± 0.37 mg PC/g), DPPH (199 ± 0.85 µM Trolox eq/g), and FRAP (243 ± 0.26 µM Trolox eq/g). Spray drying could be the most time- and energy-efficient technique for drying the GSS extract. The present study also assessed the effects of storage temperature and time on procyanidins and antioxidant activities in GSS extract powder. Procyanidins were found to degrade more rapidly at 45 °C than at 25 °C and 35 °C. Storage under 25 °C was appropriate for maintaining the procyanidin contents, DPPH, and FRAP activities in the GSS extract powder. This study contributed to the body of knowledge by explaining the preparation of procyanidin extract powder from GSS, which might be employed as a low-cost supply of nutraceutical compounds for the functional food industry and pharmaceutical sector.
Spoilage Potential of Contaminating Yeast Species Kluyveromyces marxianus, Pichia kudriavzevii and Torulaspora delbrueckii during Cold Storage of Skyr
Authors: Srimahaeak Thanyaporn , Petersen Mikael Agerlin , Lillevang Søren K. , Jespersen Lene , Larsen Nadja
Published: June 2022

This study investigated the spoilage potential of yeast strains Kluyveromyces marxianus (Km1, Km2 and Km3), Pichia kudriavzevii Pk1 and Torulaspora delbrueckii Td1 grown in skyr in cold storage. Yeast strains were isolated from skyr and identified by sequencing of the 26S rRNA gene. K. marxianus yeasts were grown in skyr to high numbers, generating large amounts of volatile organic compounds (VOC) associated with off-flavours, among them were alcohols (3-methyl-1-butanol, 2-methyl-1-propanol and 1-hexanol), esters (ethyl acetate and 3-methylbutyl acetate) and aldehydes (hexanal, methylbutanal and methylpropanal). Growth of P. kudriavzevii Pk1 led to moderate increases in several alcohols and esters (mostly, 3-methyl-1-butanol and ethyl acetate), whereas only minor shifts in VOCs were associated with T. delbrueckii Td2. The levels of the key aroma compounds, diacetyl and acetoin, were significantly decreased by all K. marxianus strains and P. kudriavzevii Pk1. In contrast to the other yeast species, K. marxianus was able to utilize lactose, producing ethanol and carbon dioxide. Based on the overall results, K. marxianus was characterised by the highest spoilage potential. The study revealed the differences between the yeast species in fermentative and spoilage activities, and clarified the role of yeast metabolites for off-flavour formation and quality defects in skyr during cold storage.
An Explorative Analysis of the Influence of Landscape Visual Aesthetic Quality on Food Preferences in Italy: A Pilot Study
Authors: Tempesta Tiziano , Vecchiato Daniel
Published: June 2022

As some previous research has highlighted, landscape characteristics are useful for improving the market share of some food products and the market power of companies in the agrifood sector. The purpose of this study is to verify whether the visual aesthetic quality of the landscape can influence food preferences and the willingness to pay for agrifood products. To this end, the preferences of 64 participants for three types of juice (orange, peach and pear) were analysed through a blind tasting experiment. Each participant tasted three pairs of fruit juices, one for each type of juice. The juices belonging to each pair were the same, but before tasting, the participants were shown two photos portraying the orchards where the fruits were produced, so participants were induced to think that the juices were different. The landscape associated with each pair of photographs had a different visual aesthetic quality (high or low). Participants were asked to provide three measures while tasting the juices: their overall juice assessment using a seven-point hedonic scale, the visual aesthetic quality of the photos on a seven-point Likert scale, and their willingness to pay as a percentage variation of the price that they usually pay to buy fruit juices. According to our results, the mean overall liking score and the mean willingness to pay percentage variation for the juices associated with a preferred landscape was higher and statistically different. Despite the need for further research, our results suggest that landscape acts as a proxy for quality in the evaluation of some food products and that the use of landscape photos could be a valid marketing strategy in agribusiness.
Extrusion Simulation for the Design of Cereal and Legume Foods
Authors: Kristiawan Magdalena , Della Valle Guy , Berzin Françoise
Published: June 2022

A 1D global twin-screw extrusion model, implemented in numerical software, Ludovic®, was applied to predict extrusion variables and, therefore, to design various starchy products with targeted structure and properties. An experimental database was built with seven starchy food formulations for manufacturing dense and expanded foods made from starches, starch blends, breakfast cereals, pulse crop ingredients such as pea flour, fava bean flour, and fava bean starch concentrated, and wheat flour enriched with wheat bran. This database includes the thermal and physical properties of the formulations at solid and molten states, melt viscosity model, extruder configurations and operating parameters, and extruded foods properties. Using extrusion and viscosity models, melt temperature (T) and specific mechanical energy (SME) were satisfactorily predicted. A sensitivity analysis of variables at die exit was performed on formulation, extruder configuration, and operating parameters, generating the extruder operating charts. Results allowed the establishment of relationships between predicted variables (T, SME, melt viscosity) and product features such as starch and protein structural change, density and cellular structure, and functional properties. The extrusion operating conditions leading to targeted food properties can be assessed from these relationships and also the relationship between extrusion operating parameters and variables provided by simulation.
Development of Simultaneous Analytical Method for Imidazolinone Herbicides from Livestock Products by UHPLC-MSMS
Authors: Heo Hyo-Min , Jo Hyeong-Wook , Chang Hee-Ra , Moon Joon-Kwan
Published: June 2022

A simultaneous analytical method, which used LC/MSMS for imidazolinone herbicides from livestock products (egg, milk, beef, pork, and chicken) for monitoring, was developed with a QuEChERS preparation. A weighed sample (5 g) in a 50 mL conical tube was added to 0.1 M potassium phosphate dibasic solution (5 mL) and shaken for 10 min. After shaking, 0.5 mL of 6 N HCl and 5 mL of acetonitrile were added, and this solution was shaken for 10 min. Additionally, QuEChERS extraction salts (original method, 4 g MgSO4, 1 g NaCl) were added to the sample in a 50 mL conical tube. The mixture was strongly shaken for 1 min and centrifuged at 3000× g for 10 min. The acetonitrile layer was purified with dSPE (150 mg MgSO4, 25 mg C18) and was centrifuged at 13,000× g for 5 min. The supernatant was filtered with a membrane filter (pore size: 0.2 μm) before analysis. The ME (%, matrix effect) range for almost all analytes was −6.56 to 7.11%. MLOD (method limit of detection) and MLOQ (method limit of quantitative) values were calculated by the S/N ratio. MLOQs were 0.01 mg/kg. The linear correlation coefficients (R2) were >0.99 with the range of 0.5~25 μg/kg for all of the imidazolinone herbicides. The recoveries (of imidazolinone herbicides) were in the range of 76.1~110.6% (0.01 mg/kg level), 89.2~97.1% (0.1 mg/kg level), and 94.4~104.4% (0.5 mg/kg level). These are within the validation criteria (to recover 70–120% with RSD <20%). The method demonstrated the simple, rapid, high throughput screening and quantitative analysis of imidazolinone herbicide residues for monitoring in livestock products.
Cow’s Milk Antigens Content in Human Milk: A Scoping Review
Authors: Franco Carlos , Fente Cristina , Sánchez Cristina , Lamas Alexandre , Cepeda Alberto , Leis Rosaura , Regal Patricia
Published: June 2022

The functionality of breast milk in terms of immunity is well-known. Despite this, a significant proportion of breastfed infants exhibit sensitization to different potentially allergenic proteins and clinical reactivity (including anaphylaxis) early in life and before the introduction of complementary feeding for the first time. The potential induction of early oral tolerance to overcome early allergic sensitization through exposure to allergens in breast milk also remains controversial and not yet well-established. The objective of this scoping review is to provide a critical appraisal of knowledge about the content of cow’s milk antigens in human milk. The amount of dietary derived milk antigens found in human milk and the analytical methodologies used to detect and quantify these antigens, the allergic status of the mother, the stage of lactation, the time of sampling (before or after ingestion of food), and the impact of human milk allergen on the infant were the outcomes that were assessed. Allergy risk was explored in all reviewed studies and could help to better elucidate its role in the context of allergic disease development. According to the included literature, we can conclude that there are mainly fragments derived from bovine proteins in human milk, and the presence of potentially allergenic molecules is greater in the milk of mothers with an allergic tendency. A clear relationship between maternal diet and allergen content in breast milk could not be firmly concluded though. Also, infants receiving milk from human milk banks, where donor milk is pasteurized for preservation, may be subject to greater risk of allergy development, especially for β-lactoglobulin.
3D Food Printing Applications Related to Dysphagia: A Narrative Review
Authors: Lorenz Tim , Iskandar Michèle M. , Baeghbali Vahid , Ngadi Michael O. , Kubow Stan
Published: June 2022

Dysphagia is a condition in which the swallowing mechanism is impaired. It is most often a result of a stroke. Dysphagia has serious consequences, including choking and aspiration pneumonia, which can both be fatal. The population that is most affected by it is the elderly. Texture-modified diets are part of the treatment plan for dysphagia. This bland, restrictive diet often contributes to malnutrition in patients with dysphagia. Both energy and protein intake are of concern, which is especially worrying, as it affects the elderly. Making texture-modified diets more appealing is one method to increase food intake. As a recent technology, 3D food printing has great potential to increase the appeal of textured foods. With extrusion-based printing, both protein and vegetable products have already been 3D printed that fit into the texture categories provided by the International Dysphagia Diet Standardization Initiative. Another exciting advancement is 4D food printing which could make foods even more appealing by incorporating color change and aroma release following a stimulus. The ultra-processed nature of 3D-printed foods is of nutritional concern since this affects the digestion of the food and negatively affects the gut microbiome. There are mitigating strategies to this issue, including the addition of hydrocolloids that increase stomach content viscosity and the addition of probiotics. Therefore, 3D food printing is an improved method for the production of texture-modified diets that should be further explored.
Purification and Characterization of a Dark Red Skin Related Dimeric Polyphenol Oxidase from Huaniu Apples
Authors: Liu Bin , Zhou Xianfang , Guan Haiyan , Pang Xuequn , Zhang Zhaoqi
Published: June 2022

The distinct dark-red skin of Huaniu apples renders them attractive to customers. However, the mechanism that leads to the development of the color of the fruit is unclear. In this study, we found that compared with red Fuji (a bright-red apple cultivar), Huaniu apples had higher contents of (−)-epicatechin (EC), (−)-epigallocatechin (EGC), (−)-gallocatechin gallate (GCG), and procyanidins (PCs) B2 and C1 in the peel, which implies that the polymerization of the flavanols and PCs may be correlated with the dark-red skin of the fruit. Using EC as a substrate, we purified an enzyme from Huaniu peel. We performed protein sequencing and discovered that the enzyme was a polyphenol oxidase (PPO). The molecular weight of the enzyme was approximately 140 kDa, which we estimated by native-PAGE and SDS-PAGE, while it was 61 kDa by urea-SDS-PAGE, from which we discovered that the PPO was a dimer. We observed the lowest Km value for catechol (0.60 mM), and the best substrate was 4-methylcatechol, with a Vmax of 526.32 U mg−1 protein. EC is a suitable natural substrate, with a Km value of 1.17 mM, and 55.27% of the Vmax/Km of 4-methylcatechol. When we used EC as a substrate, the optimum temperature and pH of the PPO were 25 °C and 5.0, respectively. In summary, we purified a dimeric PPO from Huaniu apples that showed high activity to EC, which might catalyze the polymerization of flavanols and PCs and lead to the dark-red color development of the fruit.
The Investigation of Protein Profile and Meat Quality in Bovine Longissimus thoracic Frozen under Different Temperatures by Data-Independent Acquisition (DIA) Strategy
Authors: Li Xia , Qian Shuyi , Huang Feng , Li Kaimin , Song Yu , Liu Jiqian , Guo Yujie , Zhang Chunhui , Blecker Christophe
Published: June 2022

The influence of freezing on the protein profile and quality traits in bovine Longissimus thoracic (LT) muscle was investigated by the data-independent acquisition (DIA) technique. Compared to fresh meat, a total of 262 proteins were identified as differential abundance proteins (DAPs) in four frozen groups (−12 °C, −18 °C, −38 °C, and −80 °C). According to the bioinformatics analysis, most of the DAPs in the significant Go terms and the KEGG pathway were structure proteins and enzymes. Proteome changes in the frozen bovine muscle at −12 °C and −18 °C were more significant than those at −38 °C and −80 °C. The result was consistent with the deterioration trend of the meat quality. The correlation analysis revealed that 17 proteins were correlated closely with the color, shear force, thawing loss, and cooking loss of the frozen meat, which could be used as putative biomarkers for frozen meat quality. MYO18A and ME3 are newly discovered proteins that are associated with frozen beef quality. In addition, CTTN and SERPINB6 were identified in frozen groups, which exhibited a significant inverse correlation with thawing loss (p < 0.01). These findings reveal the quality changes induced by freezing at the protein molecular level and provide new insights into the control of quality deterioration.
Bacterial Diversity and Lactic Acid Bacteria with High Alcohol Tolerance in the Fermented Grains of Soy Sauce Aroma Type Baijiu in North China
Authors: Wang Jiali , Lu Chengshun , Xu Qiang , Li Zhongyuan , Song Yajian , Zhou Sa , Zhang Tongcun , Luo Xuegang
Published: June 2022

Soy sauce aroma type baijiu (also known as Maotai-flavor baijiu) is one of the most popular types of baijiu in China. Traditionally, it is mainly produced in Southwest China. However, in recent decades, some other regions in China have also been able to produce high-quality soy sauce aroma type baijiu, but their microbial flora characteristics during fermentation are still unclear. Here, the bacterial microbial community structure of fermented grains in different rounds of Lutaichun soy sauce aroma type baijiu produced in North China was studied by high-throughput sequencing technology, and the potential probiotics strains with good characteristics (alcohol tolerance, etc.) were screened. The results showed that lactic acid bacteria were the main bacteria in the process of baijiu fermentation. However, as the number of repeated fermentation rounds increased, the proportion of lactic acid bacteria decreased. Firmicutes (96.81%) were the main bacteria in baijiu fermentation at the phylum level, and Lactobacillus (66.50%) were the main bacteria at the genus level. Finally, two strains with high resistance to alcohol stress, Lactiplantibacillus plantarum LTJ12 and Pediococcus acidilactici LTJ28, were screened from 48 strains of lactic acid bacteria in the fermented grains. The survival rates of L. plantarum LTJ12 and P. acidilactici LTJ28 under the 8% alcohol stress treatment were 59.01% and 55.50%, respectively. To the best of our knowledge, this study is the first to reveal the microbial succession of fermented grains in different rounds of soy sauce aroma type baijiu from North China, and has the benefit of explaining the deep molecular mechanism in the process of baijiu fermentation. In addition, the obtained lactic acid bacteria strains with high alcohol tolerance could be conducive to the development of new products such as active probiotic alcoholic beverages and may have important industrial development prospects also.
On the Global Well-Posedness of Rotating Magnetohydrodynamics Equations with Fractional Dissipation
Authors: Abidin Muhammad Zainul , Marwan Muhammad , Kalsoom Humaira , Omer Omer Abdalrhman
Published: June 2022

This work considers the three-dimensional incompressible rotating magnetohydrodynamics equation spaces with fractional dissipation (−Δ)℘ for 12<℘≤1. Furthermore, we use the Littlewood–Paley decomposition and frequency localization techniques to establish the global well-posedness of fractional rotating magnetohydrodynamics equations in a more generalized Besov spaces characterized by the time evolution semigroup related to the generalized linear Stokes–Coriolis operator.
Network-Based Methods for Approaching Human Pathologies from a Phenotypic Point of View
Authors: Ranea Juan A. G. , Perkins James , Chagoyen Mónica , Díaz-Santiago Elena , Pazos Florencio
Published: June 2022

Network and systemic approaches to studying human pathologies are helping us to gain insight into the molecular mechanisms of and potential therapeutic interventions for human diseases, especially for complex diseases where large numbers of genes are involved. The complex human pathological landscape is traditionally partitioned into discrete “diseases”; however, that partition is sometimes problematic, as diseases are highly heterogeneous and can differ greatly from one patient to another. Moreover, for many pathological states, the set of symptoms (phenotypes) manifested by the patient is not enough to diagnose a particular disease. On the contrary, phenotypes, by definition, are directly observable and can be closer to the molecular basis of the pathology. These clinical phenotypes are also important for personalised medicine, as they can help stratify patients and design personalised interventions. For these reasons, network and systemic approaches to pathologies are gradually incorporating phenotypic information. This review covers the current landscape of phenotype-centred network approaches to study different aspects of human diseases.
Dynamic Numerical Simulations of Dry-Stone Retaining Walls: Identification of the Seismic Behaviour Factor
Authors: Savalle Nathanaël , Vincens Eric , Hans Stéphane , Lourenço Paulo B.
Published: June 2022

Dry-stone retaining walls can be found worldwide and constitute critical assets of the built heritage for many sloped territories, holding cultural and economic value. Their design currently follows empirical rules, though the first steps towards a static safety assessment have recently been proposed in the scientific and engineering literature. However, the seismic design of these structures still lacks research studies. Therefore, this work conducts discrete element simulations to assess their dynamic behaviour. First, the approach is validated through existing scaled-down shaking table experiments, and it is found that the numerical simulations are conservative (i.e., on the safe side). Next, full-scale dry-stone retaining walls are subjected to harmonic excitations as an idealisation of earthquakes. Finally, based on a simplified limit-equilibrium analytical tool, their seismic behaviour factor is estimated for the first time in the literature, which falls within the proposed values of the European standards (Eurocode 8). This will allow engineers to adopt a validated behaviour factor in practice to assess and design dry-stone retaining walls with a pseudo-static approach.
A Comparison of Univariate and Multivariate Forecasting Models Predicting Emergency Department Patient Arrivals during the COVID-19 Pandemic
Authors: Etu Egbe-Etu , Monplaisir Leslie , Masoud Sara , Arslanturk Suzan , Emakhu Joshua , Tenebe Imokhai , Miller Joseph B. , Hagerman Tom , Jourdan Daniel , Krupp Seth
Published: June 2022

The COVID-19 pandemic has heightened the existing concern about the uncertainty surrounding patient arrival and the overutilization of resources in emergency departments (EDs). The prediction of variations in patient arrivals is vital for managing limited healthcare resources and facilitating data-driven resource planning. The objective of this study was to forecast ED patient arrivals during a pandemic over different time horizons. A secondary objective was to compare the performance of different forecasting models in predicting ED patient arrivals. We included all ED patient encounters at an urban teaching hospital between January 2019 and December 2020. We divided the data into training and testing datasets and applied univariate and multivariable forecasting models to predict daily ED visits. The influence of COVID-19 lockdown and climatic factors were included in the multivariable models. The model evaluation consisted of the root mean square error (RMSE) and mean absolute error (MAE) over different forecasting horizons. Our exploratory analysis illustrated that monthly and weekly patterns impact daily demand for care. The Holt–Winters approach outperformed all other univariate and multivariable forecasting models for short-term predictions, while the Long Short-Term Memory approach performed best in extended predictions. The developed forecasting models are able to accurately predict ED patient arrivals and peaks during a surge when tested on two years of data from a high-volume urban ED. These short- and long-term prediction models can potentially enhance ED and hospital resource planning.
Antibiotic Resistance of Helicobacter pylori in Children with Gastritis and Peptic Ulcers in Mekong Delta, Vietnam
Authors: Le Loan Thi Thuy , Nguyen Tuan Anh , Nguyen Nghia An , Nguyen Yen Thi Hai , Nguyen Hai Thi Be , Nguyen Liem Thanh , Vi Mai Tuyet , Nguyen Thang
Published: June 2022

Background: Helicobacter pylori (H. pylori) infection causes gastritis, duodenal and gastric ulcers, and gastric cancer. H. pylori eradication efficacy is low worldwide, and antibiotic resistance is the leading cause of therapy failure; therefore, this study was performed to determine the characteristics of antibiotic resistance of H. pylori in children with gastritis, duodenal and gastric ulcer. Methods: A cross-sectional study was conducted on 237 pediatric patients diagnosed with gastroduodenal inflammation and ulcer at two hospitals in Vietnam from March 2019 to April 2022. Pediatric patients with positive H. pylori tests continued to do E-tests to measure the minimum inhibitory concentration of the antibiotic so that we could prescribe effective antibiotics based on the sensitivity. Results: In 237 pediatric patients (51.1% males) with a median age of 10.3 years (range 5–16 years), endoscopic images showed that inflammatory lesions and peptic ulcers accounted for 69.2% and 30.8%, respectively. Resistance rates of H. pylori were 80.6% to clarithromycin (CLR), 71.7% to amoxicillin (AMX), 49.4% to metronidazole (MTZ), 45.1% to levofloxacin (LEV), and 11.4% to tetracycline (TET); dual resistance to AMX + CLR was 64.2%, AMX + LEV 35%, AMX + MTZ 33.3%, CLR + MTZ 32.5%, and TET + MTZ 7.2%. The frequency of clarithromycin resistance was significantly increased, particularly in pediatric patients who had received prior H. pylori treatment. The percentage of amoxicillin resistance increased with age; amoxicillin resistance of H. pylori was more prevalent among pediatric patients with peptic ulcers than those with gastroduodenal inflammation and higher in males than females. Conclusions: The proportions of resistance to CLR, AMX, MTZ, and LEV were extremely high, in contrast to TET, which was lower in pediatric patients. Our study suggests that the standard triple therapy with CLR should be limited as the empiric therapy for pediatric patients, and we should consider using eradication regimens with TET for children over 8 years of age if the medical facility is not qualified to perform antibiotic susceptibility tests of H. pylori in the Mekong Delta.
Virtual Reality in the Rehabilitation of Patients with Injuries and Diseases of Upper Extremities
Authors: Tokgöz Pinar , Stampa Susanne , Wähnert Dirk , Vordemvenne Thomas , Dockweiler Christoph
Published: June 2022

Upper-extremity injuries and diseases rarely have life-threatening consequences, but failure to manage them properly can result in severe dysfunction. This article presents the current state of using virtual reality to support the rehabilitation process of patients with injuries and diseases of the upper extremities and points out their effects on upper-extremity functions. A scoping review was conducted to provide a comprehensive overview of the field of virtual reality for upper-extremity rehabilitation. PubMed, Web of Science, and the Cochrane Library were searched by two independent researchers between April and May 2021 to identify relevant publications and were examined according to inclusion and exclusion criteria. As a result of the literature review, 11 studies of various target groups were identified. Virtual-reality technologies were categorized into multisensory high-end systems and game-based systems. With respect to functional recovery, technologies based on virtual reality were not inferior to traditional rehabilitation. In addition, the users were highly motivated and satisfied. The results emphasize the need for stronger evidence-based virtual-reality technologies for rehabilitation of injuries and diseases of upper extremities.
Association between the Development of Hospice and Palliative Care and Government-Funded Research Priority: Taiwan-Based Example
Authors: Cho Ming-Chieh , Yang Po-Chin , Wang Yueh-Hsin , Chang Hsiao-Ting , Lin Ming-Hwai
Published: June 2022

In recent years, hospice and palliative care (HPC) has grown, developed, and changed in response to the humanistic and social needs for supporting those with incurable illnesses. As a relatively new discipline, research is needed in HPC, and the priority setting of research is essential to help direct finite resources to support research. To promote creative research in different fields including HPC, the Taiwan government subsidized institutions to conduct research. In this study, we obtained data from the Government Research Bulletin, an open-source online system containing complete information about government subsidized studies since 1993 to investigate the development of research priority in HPC in Taiwan. In total, 552 studies were recorded during 1993–2021, with a continued upward trend. An association was found between research priority and the promulgation of new HPC regulations. The type of diseases in research extended from cancer to all advanced chronic conditions. The increased diversity in out-of-hospital settings of palliative research was also observed. Numerous studies have focused on education, and the theme gradually shifted from “training and education for healthcare professionals” to “public education”. Here, the results may serve as a basis to understand the commonalities of research and enhance dialog in HPC research.
Child Gender and Married Women’s Overwork: Evidence from Rural–Urban Migrants in China
Authors: Song Yanjiao , Wang Ruojing
Published: June 2022

Overwork is one of the risk factors for the work-related burden of disease. In China, nearly a quarter of migrant women are overworked. Working long hours can significantly increase the possibility of migrant women suffering from hypertension and hyperglycemia. The phenomenon of overtime work of migrant women and their health conditions deserves attention. Based on the China Migrants Dynamic Survey in 2016, this study indicates that giving birth to a boy may exacerbate overtime work among migrant women and having more boys in a family increases the probability of women’s overwork. Empirical results confirmed the existence of son preferences in China. Compared with women who gave birth to boys, women who gave birth to girls have a lower probability of being a workaholic due to a future fertility plan. Furthermore, the overwork of women is also due to the great economic burden on families to buy a wedding house, brought on by the birth of boys. To overcome the endogenous problem caused by this omitted explanatory variable, this study uses each province’s relative sex ratio at birth in 2010 as the instrumental variable of the firstborn’s gender. The IV results illustrate that the birth of boys still significantly exacerbates women’s overwork. Furthermore, considering age heterogeneity, the influence of son preference on women’s overtime work exists throughout their labor life cycle. This paper provides a new perspective for understanding migrant women’s overtime work and their health issues in urban China.
Adverse Childhood Events, Post-Traumatic Stress Disorder, Infectious Encephalopathies and Immune-Mediated Disease
Authors: Bransfield Robert C.
Published: June 2022

Adverse Childhood Events (ACE), Post-Traumatic Stress Disorder (PTSD), and infectious encephalopathies are associated with immune-mediated diseases. Data supporting this are reviewed, and an integrated hypothesis is provided. All three can be associated with intrusive symptoms and temporal lobe pathology. ACE and PTSD are accompanied by an impaired mental capacity to differentiate external danger vs. safety. Infectious encephalopathies are accompanied by a failure of adaptive immunity and an impaired immune capacity to differentiate internal danger vs. safety. All three conditions are associated with impairments to differentiate danger vs. safety and adapt effectively. There are reciprocal interactions between ACE, PTSD, and infectious encephalopathies with accompanying persistent immune activation. This is associated with immune dysregulation, chronic hyperarousal, activation of the stress response, and impairments of the fear recognition and response neural circuits, hypothalamic–pituitary–adrenal axis, amygdala, and hippocampus. The pathophysiological processes can result in a broad spectrum of chronic neuropsychiatric and somatic symptoms and diseases. Understanding the psychodynamic, neurological, neuroimmune, inflammatory and autoimmune components of this interactive process expands the effective treatment opportunities.
Hospital Length of Stay in Patients with and without Serious and Persistent Mental Illness: Evidence of Racial and Ethnic Differences
Authors: Adepoju Omolola E. , Kim Lyoung H. , Starks Steven M.
Published: June 2022

Background: Prior studies have documented racial and ethnic differences in mental healthcare utilization, and extensively in outpatient treatment and prescription medication usage for mental health disorders. However, limited studies have investigated racial and ethnic differences in length of inpatient stay (LOS) in patients with and without Serious and Persistent Mental Illness. Understanding racial and ethnic differences in LOS is necessary given that longer stays in hospital are associated with adverse health outcomes, which in turn contribute to health inequities. Objective: To examine racial and ethnic differences in length of stay among patients with and without serious and persistent mental illness (SPMI) and how these differences vary in two age cohorts: patients aged 18 to 64 and patients aged 65+. Methods: This study employed a retrospective cohort design to address the research objective, using the 2018 Healthcare Cost and Utilization Project (HCUP) National Inpatient Sample. After merging the 2018 National Inpatient Sample’s Core and Hospital files, Generalized Linear Model (GLM), adjusting for covariates, was applied to examine associations between race and ethnicity, and length of stay for patients with and without SPMI. Results: Overall, patients from racialized groups were likely to stay longer than White patients regardless of severe mental health status. Of all races and ethnicities examined, Asian patients had the most extended stays in both age cohorts: 8.69 days for patients with SPMI and 5.73 days for patients without SPMI in patients aged 18 to 64 years and 8.89 days for patients with SPMI and 6.05 days for patients without SPMI in the 65+ cohort. For individuals aged 18 to 64, differences in length of stay were significantly pronounced in Asian patients (1.6 days), Black patients (0.27 days), and Native American patients/patients from other races (0.76 days) if they had SPMI. For individuals aged 65 and older, Asian patients (1.09 days) and Native American patients/patients from other races (0.45 days) had longer inpatient stays if they had SPMI. Conclusion: Racial and ethnic differences in inpatient length of stay were most pronounced in Asian patients with and without SPMI. Further studies are needed to understand the mechanism(s) for these differences.
Acute Supplementation with Cannabidiol Does Not Attenuate Inflammation or Improve Measures of Performance following Strenuous Exercise
Authors: Crossland Brett W. , Rigby B. Rhett , Duplanty Anthony A. , King George A. , Juma Shanil , Levine Nicholas A. , Clark Cayla E. , Ramirez Kyndall P. , Varone Nicole L.
Published: June 2022

Supplementation with cannabidiol (CBD) may expedite recovery when consumed after exercise. The purpose of this study was to determine if supplementation with CBD reduces inflammation and enhances performance following strenuous eccentric exercise in collegiate athletes. Twenty-four well-trained females (age = 21.2 ± 1.8 years, height = 166.4 ± 8 cm, weight = 64.9 ± 9.1 kg) completed 100 repetitions of unilateral eccentric leg extension to induce muscle damage. In this crossover design, participants were randomized to receive 5 mg/kg of CBD in pill form or a placebo 2 h prior to, immediately following, and 10 h following muscle damage. Blood was collected, and performance and fatigue were measured prior to, and 4 h, 24 h, and 48 h following the muscle damage. Approximately 28 days separated treatment administration to control for the menstrual cycle. No significant differences were observed between the treatments for inflammation, muscle damage, or subjective fatigue. Peak torque at 60°/s (p = 0.001) and peak isometric torque (p = 0.02) were significantly lower 24 h following muscle damage, but no difference in performance was observed between treatments at any timepoint. Cannabidiol supplementation was unable to reduce fatigue, limit inflammation, or restore performance in well-trained female athletes.
Medical–Legal and Psychosocial Considerations on Parental Alienation as a Form of Child Abuse: A Brief Review
Authors: Isailă Oana-Maria , Hostiuc Sorin
Published: June 2022

Parental alienation, an entity situated at the limit of psychiatry, sociology, and justice, still represents a controversial concept despite the legal dispositions that take it into account. The scope of this paper is to consider the relationship between parent and child, and child abuse from a psychosocial perspective, as well as to depict parental alienation, considered a form of child abuse, without omitting contradictory arguments which are also based on prudence in the minor’s interest, turning the attention to parental estrangement. Although parental alienation is not a psychiatric diagnosis per se and neither is parental estrangement, recognizing the difference between them is vital to adequately manage the situation at the time of establishing custody.
The Link between Mineral Elements Variation and Internal Flesh Breakdown of ‘Keitt’ Mango in a Steep Slope Mountain Area, Southwest China
Authors: Ma Xiaowei , Wang Jianfang , Su Muqing , Liu Bin , Du Bang , Zhang Yuehua , He Liping , Wang Songbiao , Wu Hongxia
Published: June 2022

Internal flesh breakdown (IFB), a serious physiological disorder of mango fruit, causes significant economic losses in Southwest China. We investigated the extent of IFB in 100 mango orchards and how changes in the mineral nutrients of fruit flesh, leaves and soil affect IFB. We found that 76% of the mango orchards showed IFB symptoms, and the average IFB incidence was 10%. Fruit flesh with IFB showed higher average contents of N, P, K and Mg, lower average Ca content and higher average ratios of N/Ca, K/Ca and Mg/Ca. The leaves from orchards with IFB symptoms exhibited a remarkable increase in the average N and Mg contents. No significant difference was observed in the soil nutrient concentrations between orchards with and without IFB fruit. IFB incidence was significantly positively correlated with the N/Ca, K/Ca and Mg/Ca ratios in the fruit flesh. However, when considering individual orchards with IFB symptoms, fruit flesh that exhibited breakdown symptoms had Ca content higher, lower than or equal to that of the healthy fruit flesh. There was a strong correlation between fruit flesh and leaf in the same mineral elements, but neither of them showed a significant correlation with soil. Considering the mango trees were cultivated on steep slopes, and fertilizer was applied at a fixed position, we hypothesized that long-term fertilization in the partial root zone led to the excess of N, K and Mg in soil, which reduced the total flesh Ca content or resulted in the abnormal cellular distribution of Ca in the flesh, and ultimately triggered IFB development.
Endophytic Yeasts for the Biocontrol of Phlyctema vagabunda in Apples
Authors: Sepúlveda Ximena , Silva Diego , Ceballos Ricardo , Vero Silvana , López María Dolores , Vargas Marisol
Published: June 2022

Bull’s-eye rot, produced by Phlyctema vagabunda, is an important postharvest disease in apples. Current measures to control infection include synthetic fungicides, in addition to the application of copper hydroxide and potassium phosphite. However, growing public concern regarding fungicide residues in food has generated interest in developing non-chemical alternative control methods; biological control is one of the most promising alternatives. In this research, native endophytic yeasts were isolated and evaluated for the biocontrol of P. vagabunda in apples. The mechanisms of action involved were also determined. Our research found 2 isolates, Vishniacozyma victoriae EPL4.5 and EPL29.5, which exhibited biocontrol activity against P. vagabunda at 20 °C in apples, the incidence of bull’s-eye rot was reduced by 39% and 61%, respectively, and the severity of the disease was decreased by 67% and 70%, respectively, when apples were inoculated with these yeasts 24 h before applying the pathogen. The main mechanisms that could be involved in the observed biocontrol activity are the ability to form biofilms and the production of volatile organic compounds.