bluuebunny/crossref_metadata_2025 huggingface dataset:
Unusually Paced Life History Strategies of Marine Megafauna Drive Atypical Sensitivities to Environmental Variability
Authors: Smallegange Isabel M. , Flotats Avilés Marta , Eustache Kim
Published: January 2021

Understanding why different life history strategies respond differently to changes in environmental variability is necessary to be able to predict eco-evolutionary population responses to change. Marine megafauna display unusual combinations of life history traits. For example, rays, sharks and turtles are all long-lived, characteristic of slow life histories. However, turtles also have very high reproduction rates and juvenile mortality, characteristic of fast life histories. Sharks and rays, in contrast, produce a few live-born young, which have low mortality rates, characteristic of slow life histories. This raises the question if marine megafaunal responses to environmental variability follow conventional life history patterns, including the pattern that fast life histories are more sensitive to environmental autocorrelation than slow life histories. To answer this question, we used a functional trait approach to quantify for different species of mobulid rays, cheloniid sea turtles and carcharhinid sharks – all inhabitants or visitors of (human-dominated) coastalscapes – how their life history, average size and log stochastic population growth rate, log(λs), respond to changes in environmental autocorrelation and in the frequency of favorable environmental conditions. The faster life histories were more sensitive to temporal frequency of favourable environmental conditions, but both faster and slower life histories were equally sensitive, although of opposite sign, to environmental autocorrelation. These patterns are atypical, likely following from the unusual life history traits that the megafauna display, as responses were linked to variation in mortality, growth and reproduction rates. Our findings signify the importance of understanding how life history traits and population responses to environmental change are linked. Such understanding is a basis for accurate predictions of marine megafauna population responses to environmental perturbations like (over)fishing, and to shifts in the autocorrelation of environmental variables, ultimately contributing toward bending the curve on marine biodiversity loss.
Demultiplexing Infrasound Phonons With Tunable Magnetic Lattices
Authors: Watkins Audrey A. , Bilal Osama R.
Published: January 2021

Controlling infrasound signals is crucial to many processes ranging from predicting atmospheric events and seismic activities to sensing nuclear detonations. These waves can be manipulated through phononic crystals and acoustic metamaterials. However, at such ultra-low frequencies, the size (usually on the order of meters) and the mass (usually on the order of many kilograms) of these materials can hinder its potential applications in the infrasonic domain. Here, we utilize tunable lattices of repelling magnets to guide and sort infrasound waves into different channels based on their frequencies. We construct our lattices by confining meta-atoms (free-floating macroscopic disks with embedded magnets) within a magnetic boundary. By changing the confining boundary, we control the meta-atoms’ spacing and therefore the intensity of their coupling potentials and wave propagation characteristics. As a demonstration of principle, we present the first experimental realization of an infrasound phonon demultiplexer (i.e., guiding ultra-low frequency waves into different channels based on their frequencies). The realized platform can be utilized to manipulate ultra-low frequency waves, within a relatively small volume, while utilizing negligible mass. In addition, the self-assembly nature of the meta-atoms can be key in creating re-programmable materials with exceptional nonlinear properties.
Survival Factors and Metabolic Pathogenesis in Elderly Patients (≥65) With COVID-19: A Multi-Center Study
Authors: Mei Qi , Wang Amanda Y. , Bryant Amy , Yang Yang , Li Ming , Wang Fei , Du Shangming , Kurts Christian , Wu Patrick , Ma Ke , Wu Liang , Chen Huawen , Luo Jinlong , Li Yong , Hu Guangyuan , Yuan Xianglin , Li Jian
Published: January 2021

Background: Elderly patients infected with COVID-19 are reported to be facing a substantially increased risk of mortality. Clinical characteristics, treatment options, and potential survival factors remain under investigation. This study aimed to fill this gap and provide clinically relevant factors associated with survival of elderly patients with COVID-19.

Methods: In this multi-center study, elderly patients (age ≥65 years old) with laboratory-confirmed COVID-19 from 4 Wuhan hospitals were included. The clinical end point was hospital discharge or deceased with last date of follow-up on Jul. 08, 2020. Clinical, demographic, and laboratory data were collected. Univariate and multivariate analysis were performed to analyze survival and risk factors. A metabolic flux analysis using a large-scale molecular model was applied to investigate the pathogenesis of SARS-CoV-2 with regard to metabolism pathways.

Results: A total of 223 elderly patients infected with COVID-19 were included, 91 (40.8%) were discharged and 132 (59.2%) deceased. Acute respiratory distress syndrome (ARDS) developed in 140 (62.8%) patients, 23 (25.3%) of these patients survived. Multivariate analysis showed that potential risk factors for mortality were elevated D-Dimer (odds ratio: 1.13 [95% CI 1.04 - 1.22], p = 0.005), high immune-related metabolic index (6.42 [95% CI 2.66–15.48], p < 0.001), and increased neutrophil-to-lymphocyte ratio (1.08 [95% 1.03–1.13], p < 0.001). Elderly patients receiving interferon atmotherapy showed an increased probability of survival (0.29 [95% CI 0.17–0.51], p < 0.001). Based on these factors, an algorithm (AlgSurv) was developed to predict survival for elderly patients. The metabolic flux analysis showed that 12 metabolic pathways including phenylalanine (odds ratio: 28.27 [95% CI 10.56–75.72], p < 0.001), fatty acid (15.61 [95% CI 6.66–36.6], p < 0.001), and pyruvate (12.86 [95% CI 5.85–28.28], p < 0.001) showed a consistently lower flux in the survivors vs. the deceased subgroup. This may reflect a key pathogenic mechanism of COVID-19 infection.

Conclusion: Several factors such as interferon atmotherapy and recreased activity of specific metabolic pathways were found to be associated with survival of elderly patients. Based on these findings, a survival algorithm (AlgSurv) was developed to assist the clinical stratification for elderly patients. Dysregulation of the metabolic pathways revealed in this study may aid in the drug and vaccine development against COVID-19.
Analysis of Microbial Water Contamination, Soil Microbial Community Structure, and Soil Respiration in a Collaborative First-Year Students as Scholars Program (SAS)
Authors: Stiemsma Leah T. , Davis Stephen D. , Brewster Jay L.
Published: January 2021

The persistence of college students in STEM majors after their first-year of college is approximately 50%, with underrepresented populations displaying even higher rates of departure. For many undergraduates, their first-year in college is defined by large class sizes, poor access to research faculty, and minimal standing in communities of scholars. Pepperdine University and Whittier College, funded by a National Science Foundation award to Improve Undergraduate Stem Education (NSF IUSE), partnered in the development of first-year classes specifically geared to improve student persistence in STEM and academic success. This Students as Scholars Program (SAS) engaged first-year undergraduates in scholarly efforts during their first semester in college with a careful approach to original research design and mentoring by both faculty and upperclassmen experienced in research. Courses began by introducing hypothesis formulation and experimental design partnered with the scientific focus of each course (ecological, biochemical, microbiological). Students split into research teams, explored the primary literature, designed research projects, and executed experiments over a 6–7 week period, collecting, analyzing, and interpreting data. Microbiology-specific projects included partnerships with local park managers to assess water quality and microbial coliform contamination at specified locations in a coastal watershed. In addition, students explored the impact of soil salinity on microbial community structure. Analysis of these samples included next-generation sequencing and microbiome compositional analysis via collaboration with students from an upper division microbiology course. This cross-course collaboration facilitated additional student mentoring opportunities between upperclassmen and first-year students. This approach provided first-year students an introduction to the analysis of complex data sets using bioinformatics and statistically reliable gas-exchange replicates. Assessment of the impact of this program revealed students to view the research as challenging, but confidence building as they take their first steps as biology majors. In addition, the direct mentorship of first-year students by upperclassmen and faculty was viewed positively by students. Ongoing assessments have revealed SAS participants to display a 15% increased persistence rate in STEM fields when compared to non-SAS biology majors.
Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates
Authors: Liu Yang , Li Shuo , Sun Huapeng , Pan Liangqi , Cui Xinxin , Zhu Xuhui , Feng Yaling , Li Mingliang , Yu Yanan , Wu Meihua , Lin Jiate , Xu Fengxiang , Yuan Shaohua , Huang Shujian , Sun Hailiang , Liao Ming
Published: January 2021

Currently, H9N2 avian influenza viruses (H9N2 AIVs) globally circulate in poultry and have acquired some adaptation to mammals. However, it is not clear what the molecular basis is for the variation in receptor-binding features of the H9N2 AIVs. The receptor-binding features of 92 H9N2 AIVs prevalent in China during 1994–2017 were characterized through solid-phase ELISA assay and reverse genetics. H9N2 AIVs that circulated in this period mostly belonged to clade h9.4.2. Two increasing incidents occurred in the ability of H9N2 AIVs to bind to avian-like receptors in 2002–2005 and 2011–2014. Two increasing incidents occurred in the strength of H9N2 AIVs to bind to human-like receptors in 2002–2005 and 2011–2017. We found that Q227M, D145G/N, S119R, and R246K mutations can significantly increase H9N2 AIVs to bind to both avian- and human-like receptors. A160D/N, Q156R, T205A, Q226L, V245I, V216L, D208E, T212I, R172Q, and S175N mutations can significantly enhance the strength of H9N2 AIVs to bind to human-like receptors. Our study also identified mutations T205A, D208E, V216L, Q226L, and V245I as the key sites leading to enhanced receptor binding of H9N2 AIVs during 2002–2005 and mutations S119R, D145G, Q156R, A160D, T212I, Q227M, and R246K as the key sites leading to enhanced receptor binding of H9N2 AIVs during 2011–2017. These findings further illustrate the receptor-binding characteristics of avian influenza viruses, which can be a potential threat to public health.
Outer Membrane Vesicles Released From Aeromonas Strains Are Involved in the Biofilm Formation
Authors: Seike Soshi , Kobayashi Hidetomo , Ueda Mitsunobu , Takahashi Eizo , Okamoto Keinosuke , Yamanaka Hiroyasu
Published: January 2021

Aeromonas spp. are Gram-negative rod-shaped bacteria ubiquitously distributed in diverse water sources. Several Aeromonas spp. are known as human and fish pathogens. Recently, attention has been focused on the relationship between bacterial biofilm formation and pathogenicity or drug resistance. However, there have been few reports on biofilm formation by Aeromonas. This study is the first to examine the in vitro formation and components of the biofilm of several Aeromonas clinical and environmental strains. A biofilm formation assay using 1% crystal violet on a polystyrene plate revealed that most Aeromonas strains used in this study formed biofilms but one strain did not. Analysis of the basic components contained in the biofilms formed by Aeromonas strains confirmed that they contained polysaccharides containing GlcNAc, extracellular nucleic acids, and proteins, as previously reported for the biofilms of other bacterial species. Among these components, we focused on several proteins fractionated by SDS-PAGE and determined their amino acid sequences. The results showed that some proteins existing in the Aeromonas biofilms have amino acid sequences homologous to functional proteins present in the outer membrane of Gram-negative bacteria. This result suggests that outer membrane components may affect the biofilm formation of Aeromonas strains. It is known that Gram-negative bacteria often release extracellular membrane vesicles from the outer membrane, so we think that the outer membrane-derived proteins found in the Aeromonas biofilms may be derived from such membrane vesicles. To examine this idea, we next investigated the ability of Aeromonas strains to form outer membrane vesicles (OMVs). Electron microscopic analysis revealed that most Aeromonas strains released OMVs outside the cells. Finally, we purified OMVs from several Aeromonas strains and examined their effect on the biofilm formation. We found that the addition of OMVs dose-dependently promoted biofilm formation, except for one strain that did not form biofilms. These results suggest that the OMVs released from the bacterial cells are closely related to the biofilm formation of Aeromonas strains.
First Report on the Rapid Detection and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) in Viable but Non-culturable (VBNC) Under Food Storage Conditions
Authors: Ou Aifen , Wang Kan , Mao Yanxiong , Yuan Lei , Ye Yanrui , Chen Ling , Zou Yimin , Huang Tengyi
Published: January 2021

Formation of viable but non-culturable (VBNC) status in methicillin-resistant Staphylococcus aureus (MRSA) has never been reported, and it poses a significant concern for food safety. Thus, this study aimed to firstly develop a rapid, cost-effective, and efficient testing method to detect and differentiate MRSA strains in the VBNC state and further apply this in real food samples. Two targets were selected for detection of MRSA and toxin, and rapid isothermal amplification detection assays were developed based on cross-priming amplification methodology. VBNC formation was performed for MRSA strain in both pure culture and in artificially contaminated samples, then propidium monoazide (PMA) treatment was further conducted. Development, optimization, and evaluation of PMA-crossing priming amplification (CPA) were further performed on detection of MRSA in the VBNC state. Finally, application of PMA-CPA was further applied for detection on MRSA in the VBNC state in contaminated food samples. As concluded in this study, formation of the VBNC state in MRSA strains has been verified, then two PMA-CPA assays have been developed and applied to detect MRSA in the VBNC state from pure culture and food samples.
Exploring the Potential of Carbon Dots to Combat COVID-19
Authors: Kotta Sabna , Aldawsari Hibah Mubarak , Badr-Eldin Shaimaa M. , Alhakamy Nabil A. , Md Shadab , Nair Anroop B. , Deb Pran Kishore
Published: January 2021

Viral diseases are considered as a global burden. The eradication of viral diseases is always a challenging task in medical research due to the high infectivity and mutation capability of the virus. The ongoing COVID-19 pandemic is still not under control even after several months of the first reported case and global spread. Neither a specific drug nor a vaccine is available for public use yet. In the pursuit of a promising strategy, carbon dots could be considered as potential nanostructure against this viral pandemic. This review explores the possibility of carbon nano-dots to combat COVID-19 based on some reported studies. Carbon dots are photoluminescent carbon nanoparticles, smaller than 10 nm in dimension with a very attractive photostable and biocompatible properties which can be surfaced modified or functionalized. These photoluminescent tiny particles have captured much attention owing to their functionalization property and biocompatibility. In response to this pandemic outbreak, this review attempts to summarize the potential use of carbon dots in antiviral therapy with particular emphasis on their probable role in the battlefront against COVID-19 including their possible biosensing applications.
A Manufacturing-Oriented Intelligent Vision System Based on Deep Neural Network for Object Recognition and 6D Pose Estimation
Authors: Liang Guoyuan , Chen Fan , Liang Yu , Feng Yachun , Wang Can , Wu Xinyu
Published: January 2021

Nowadays, intelligent robots are widely applied in the manufacturing industry, in various working places or assembly lines. In most manufacturing tasks, determining the category and pose of parts is important, yet challenging, due to complex environments. This paper presents a new two-stage intelligent vision system based on a deep neural network with RGB-D image inputs for object recognition and 6D pose estimation. A dense-connected network fusing multi-scale features is first built to segment the objects from the background. The 2D pixels and 3D points in cropped object regions are then fed into a pose estimation network to make object pose predictions based on fusion of color and geometry features. By introducing the channel and position attention modules, the pose estimation network presents an effective feature extraction method, by stressing important features whilst suppressing unnecessary ones. Comparative experiments with several state-of-the-art networks conducted on two well-known benchmark datasets, YCB-Video and LineMOD, verified the effectiveness and superior performance of the proposed method. Moreover, we built a vision-guided robotic grasping system based on the proposed method using a Kinova Jaco2 manipulator with an RGB-D camera installed. Grasping experiments proved that the robot system can effectively implement common operations such as picking up and moving objects, thereby demonstrating its potential to be applied in all kinds of real-time manufacturing applications.
Impact of Tau on Neurovascular Pathology in Alzheimer's Disease
Authors: Canepa Elisa , Fossati Silvia
Published: January 2021

Alzheimer's disease (AD) is a chronic neurodegenerative disorder and the most prevalent cause of dementia. The main cerebral histological hallmarks are represented by parenchymal insoluble deposits of amyloid beta (Aβ plaques) and neurofibrillary tangles (NFT), intracellular filamentous inclusions of tau, a microtubule-associated protein. It is well-established that cerebrovascular dysfunction is an early feature of AD pathology, but the detrimental mechanisms leading to blood vessel impairment and the associated neurovascular deregulation are not fully understood. In 90% of AD cases, Aβ deposition around the brain vasculature, known as cerebral amyloid angiopathy (CAA), alters blood brain barrier (BBB) essential functions. While the effects of vascular Aβ accumulation are better documented, the scientific community has only recently started to consider the impact of tau on neurovascular pathology in AD. Emerging compelling evidence points to transmission of neuronal tau to different brain cells, including astrocytes, as well as to the release of tau into brain interstitial fluids, which may lead to perivascular neurofibrillar tau accumulation and toxicity, affecting vessel architecture, cerebral blood flow (CBF), and vascular permeability. BBB integrity and functionality may therefore be impacted by pathological tau, consequentially accelerating the progression of the disease. Tau aggregates have also been shown to induce mitochondrial damage: it is known that tau impairs mitochondrial localization, distribution and dynamics, alters ATP and reactive oxygen species production, and compromises oxidative phosphorylation systems. In light of this previous knowledge, we postulate that tau can initiate neurovascular pathology in AD through mitochondrial dysregulation. In this review, we will explore the literature investigating tau pathology contribution to the malfunction of the brain vasculature and neurovascular unit, and its association with mitochondrial alterations and caspase activation, in cellular, animal, and human studies of AD and tauopathies.
Interplay Between EGFR and the Platelet-Activating Factor/PAF Receptor Signaling Axis Mediates Aggressive Behavior of Cervical Cancer
Authors: Souza Juliana L. , Martins-Cardoso Karina , Guimarães Isabella S. , de Melo Andréia C. , Lopes Angela H. , Monteiro Robson Q. , Almeida Vitor H.
Published: January 2021

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase widely expressed in cervical tumors, being correlated with adverse clinical outcomes. EGFR may be activated by a diversity of mechanisms, including transactivation by G-protein coupled receptors (GPCRs). Studies have also shown that platelet-activating factor (PAF), a pro-inflammatory phospholipid mediator, plays an important role in the cancer progression either by modulating the cancer cells or the tumor microenvironment. Most of the PAF effects seem to be mediated by the interaction with its receptor (PAFR), a member of the GPCRs family. PAFR- and EGFR-evoked signaling pathways contribute to tumor biology; however, the interplay between them remains uninvestigated in cervical cancer. In this study, we employed The Cancer Genome Atlas (TCGA) and cancer cell lines to evaluate possible cooperation between EGFR, PAFR, and lysophosphatidylcholine acyltransferases (LPCATs), enzymes involved in the PAF biosynthesis, in the context of cervical cancer. It was observed a strong positive correlation between the expression of EGFR × PAFR and EGFR × LPCAT2 in 306 cervical cancer samples. The increased expression of LPCAT2 was significantly correlated with poor overall survival. Activation of EGFR upregulated the expression of PAFR and LPCAT2 in a MAPK-dependent fashion. At the same time, PAF showed the ability to transactivate EGFR leading to ERK/MAPK activation, cyclooxygenase-2 (COX-2) induction, and cell migration. The positive crosstalk between the PAF-PAFR axis and EGFR demonstrates a relevant linkage between inflammatory and growth factor signaling in cervical cancer cells. Finally, combined PAFR and EGFR targeting treatment impaired clonogenic capacity and viability of aggressive cervical cancer cells more strongly than each treatment separately. Collectively, we proposed that EGFR, LPCAT2, and PAFR emerge as novel targets for cervical cancer therapy.
Potential Importance of Early Focal Radiotherapy Following Gross Total Resection for Long-Term Survival in Children With Embryonal Tumors With Multilayered Rosettes
Authors: Mayr Lisa , Gojo Johannes , Peyrl Andreas , Azizi Amedeo A. , Stepien Natalia M. , Pletschko Thomas , Czech Thomas , Dorfer Christian , Lambo Sander , Dieckmann Karin , Haberler Christine , Kool Marcel , Slavc Irene
Published: January 2021

Embryonal tumor with multilayered rosettes (ETMR) is a rare, aggressive embryonal central nervous system tumor characterized by LIN28A expression and alterations in the C19MC locus. ETMRs predominantly occur in young children, have a dismal prognosis, and no definitive treatment guidelines have been established. We report on nine consecutive patients and review the role of initiation/timing of radiotherapy on survival. Between 2006 and 2018, nine patients were diagnosed with ETMR. Diagnosis was confirmed histopathologically, immunohistochemically and molecularly. Median age was 25 months (5–38). Location was supratentorial in five, pineal in three, and brainstem in one. Seven patients had a gross total resection, one a partial resection and one a biopsy at initial diagnosis. Chemotherapy augmented with intrathecal therapy started a median of 10 days (7–20) after surgery. Only two patients who after gross total resection received radiotherapy very early on (six weeks after diagnosis) are alive and in complete remission 56 and 50 months after diagnosis. All remaining patients for whom radiotherapy was deferred until the end of chemotherapy recurred, albeit none with leptomeningeal disease. A literature research identified 228 patients with ETMR. Including our patients only 26 (11%) of 237 patients survived >36 months with no evidence of disease at last follow-up. All but two long-term (>36 months) survivors received radiotherapy, ten of whom early on following gross total resection (GTR). GTR followed by early focal radiotherapy and intrathecal therapy to prevent leptomeningeal disease are potentially important to improve survival of ETMR in the absence of effective targeted therapies.
The Metabolic Mechanisms of Breast Cancer Metastasis
Authors: Wang Lingling , Zhang Shizhen , Wang Xiaochen
Published: January 2021

Breast cancer is one of the most common malignancy among women worldwide. Metastasis is mainly responsible for treatment failure and is the cause of most breast cancer deaths. The role of metabolism in the progression and metastasis of breast cancer is gradually being emphasized. However, the regulatory mechanisms that conduce to cancer metastasis by metabolic reprogramming in breast cancer have not been expounded. Breast cancer cells exhibit different metabolic phenotypes depending on their molecular subtypes and metastatic sites. Both intrinsic factors, such as MYC amplification, PIK3CA, and TP53 mutations, and extrinsic factors, such as hypoxia, oxidative stress, and acidosis, contribute to different metabolic reprogramming phenotypes in metastatic breast cancers. Understanding the metabolic mechanisms underlying breast cancer metastasis will provide important clues to develop novel therapeutic approaches for treatment of metastatic breast cancer.
Bufei Jianpi Formula Improves Mitochondrial Function and Suppresses Mitophagy in Skeletal Muscle via the Adenosine Monophosphate-Activated Protein Kinase Pathway in Chronic Obstructive Pulmonary Disease
Authors: Mao Jing , Li Ya , Feng Suxiang , Liu Xuefang , Tian Yange , Bian Qingqing , Li Junzi , Hu Yuanyuan , Zhang Lanxi , Ji Huige , Li Suyun
Published: January 2021

Skeletal muscle dysfunction, a striking systemic comorbidity of chronic obstructive pulmonary disease (COPD), is associated with declines in activities of daily living, reductions in health status and prognosis, and increases in mortality. Bufei Jianpi formula (BJF), a traditional Chinese herbal formulation, has been shown to improve skeletal muscle tension and tolerance via inhibition of cellular apoptosis in COPD rat models. This study aimed to investigate the mechanisms by which BJF regulates the adenosine monophosphate-activated protein kinase (AMPK) pathway to improve mitochondrial function and to suppress mitophagy in skeletal muscle cells. Our study showed that BJF repaired lung function and ameliorated pathological impairment in rat lung and skeletal muscle tissues. BJF also improved mitochondrial function and reduced mitophagy via the AMPK signaling pathway in rat skeletal muscle tissue. In vitro, BJF significantly improved cigarette smoke extract-induced mitochondrial functional impairment in L6 skeletal muscle cells through effects on mitochondrial membrane potential, mitochondrial permeability transition pores, adenosine triphosphate production, and mitochondrial respiration. In addition, BJF led to upregulated expression of mitochondrial biogenesis markers, including AMPK-α, PGC-1α, and TFAM and downregulation of mitophagy markers, including LC3B, ULK1, PINK1, and Parkin, with increased expression of downstream markers of the AMPK pathway, including mTOR, PPARγ, and SIRT1. In conclusion, BJF significantly improved skeletal muscle and mitochondrial function in COPD rats and L6 cells by promoting mitochondrial biogenesis and suppressing mitophagy via the AMPK pathway. This study suggests that BJF may have therapeutic potential for prophylaxis and treatment of skeletal muscle dysfunction in patients with COPD.
It’s All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks
Authors: Reicher Naama , Melkman-Zehavi Tal , Dayan Jonathan , Uni Zehava
Published: January 2021

The small intestine (SI) of chicks (Gallus gallus) matures rapidly during the initial post-hatch period and acquires digestive, absorptive, and secretive capabilities. The effects of the timing of first feeding on the quantities and distribution of specialized epithelial cells, which generate and maintain SI morphology and functionality, have not yet been examined. In this study, we identified specialized SI epithelial cell sub-types, including stem, progenitor, proliferating, and differentiated cells within crypts and villi of chicks during the first 10 days post-hatch, byin situhybridization (ISH), immunofluorescence (IF), and histochemical staining. We then examined their quantities and ratios between day of hatch and d10 in chicks that were fed upon hatch [early feeding (EF)], compared to chicks that were fed 24 h post-hatch [delayed feeding (DF)]. Results showed that EF increased total cell quantities in the crypts and villi at days 1, 3, 7, and 10, compared to DF (p< 0.0001). At d3, EF, in comparison to DF, decreased crypt stem cell proportions (p< 0.0001), increased crypt proliferating (p< 0.01) and differentiated (p< 0.05) cell proportions, and increased villus enterocyte proportions (p< 0.01). By d10, EF increased both the quantities and proportions of villus enterocytes and goblet cells, compared to DF. We conclude that feeding upon hatch, compared to 24 h-delayed feeding, enhanced SI maturation and functionality by increasing the quantities and proportions of proliferating and differentiated cells, thus expanding the digestive, absorptive, and secretive cell populations throughout the initial post-hatch period.
Role of Exogenous and Endogenous Hydrogen Sulfide (H2S) on Functional Traits of Plants Under Heavy Metal Stresses: A Recent Perspective
Authors: Arif Muhammad Saleem , Yasmeen Tahira , Abbas Zohaib , Ali Shafaqat , Rizwan Muhammad , Aljarba Nada H. , Alkahtani Saad , Abdel-Daim Mohamed M.
Published: January 2021

Improving growth and productivity of plants that are vulnerable to environmental stresses, such as heavy metals, is of significant importance for meeting global food and energy demands. Because heavy metal toxicity not only causes impaired plant growth, it has also posed many concerns related to human well-being, so mitigation of heavy metal pollution is a necessary priority for a cleaner environment and healthier world. Hydrogen sulfide (H2S), a gaseous signaling molecule, is involved in metal-related oxidative stress mitigation and increased stress tolerance in plants. It performs multifunctional roles in plant growth regulation while reducing the adverse effects of abiotic stress. Most effective function of H2S in plants is to eliminate metal-related oxidative toxicity by regulating several key physiobiochemical processes. Soil pollution by heavy metals presents significant environmental challenge due to the absence of vegetation cover and the resulting depletion of key soil functions. However, the use of stress alleviators, such as H2S, along with suitable crop plants, has considerable potential for an effective management of these contaminated soils. Overall, the present review examines the imperative role of exogenous application of different H2S donors in reducing HMs toxicity, by promoting plant growth, stabilizing their physiobiochemical processes, and upregulating antioxidative metabolic activities. In addition, crosstalk of different growth regulators with endogenous H2S and their contribution to the mitigation of metal phytotoxicity have also been explored.
Mini Review: Transport of Hydrophobic Polymers Into the Plant Apoplast
Authors: Xin Anzhou , Herburger Klaus
Published: January 2021

The plant apoplast contains the four hydrophobic polymer, lignin, suberin, cutin, and cutan, that are crucial for stress resistance, controlling solute diffusion, and strengthening the cell wall. Some of these polymers are widely used in industry and daily life products, such as all wood-containing goods (lignin) and wine cork (suberin). Despite the importance of these polymers, several aspects of their formation remain unknown. This mini review highlights technical bottlenecks in the current research and summarizes recent insights into the precursor transmembrane transport, an essential step in the polymer formation. We also briefly discuss how some of the remaining knowledge gaps can be closed and how a better understanding of these biopolymers will benefit other research fields.
Ring/U-Box Protein AtUSR1 Functions in Promoting Leaf Senescence Through JA Signaling Pathway in Arabidopsis
Authors: Zhang Zenglin , Xu Mengmeng , Guo Yongfeng
Published: January 2021

Leaf senescence is regulated by a large number of internal and environmental factors. Here, we report that AtUSR1 (U-box Senescence Related 1) which encodes a plant Ring/U-box protein, is involved in age-dependent and dark-induced leaf senescence in Arabidopsis. Expression of AtUSR1 gene in leaves was up-regulated in darkness and during aging. Plants of usr1, an AtUSR1 gene knock-down mutant, showed a significant delay in age-dependent and dark-induced leaf senescence and the delayed senescence phenotype was rescued when the AtUSR1 gene was transferred back to the mutant plants. Meanwhile, overexpression of AtUSR1 caused accelerated leaf senescence. Furthermore, the role of AtUSR1 in regulating leaf senescence is related to MYC2-mediuated jasmonic acid (JA) signaling pathway. MeJA treatments promoted the accumulation of AtUSR1 transcripts and this expression activation was dependent on the function of MYC2, a key transcription factor in JA signaling. Dual-luciferase assay results indicated that MYC2 promoted the expression of AtUSR1. Overexpression of AtUSR1 in myc2 mutant plants showed precocious senescence, while myc2 mutation alone caused a delay in leaf senescence, suggesting that AtUSR1 functions downstream to MYC2 in the JA signaling pathway in promoting leaf senescence.
From Autonomy Support and Grit to Satisfaction With Life Through Self-Determined Motivation and Group Cohesion in Higher Education
Authors: Lozano-Jiménez José Eduardo , Huéscar Elisa , Moreno-Murcia Juan Antonio
Published: January 2021

Using the Self-Determination Theory as a framework, this study tests the predictive capacity of the teacher’s interpersonal style of autonomy support at a higher education institution, and the grit on the satisfaction of basic psychological needs, intrinsic motivation, group cohesion, and life satisfaction in university students. A sample composed of 489 Colombian university students (381 women and 108 men), aged between 18 and 41 years (M = 21.93; DT = 3.58), was used; they filled in the questionnaires that measured the variables of interest. After the analysis of structural equations, the results showed that the perception of teaching style of autonomy support and the grit positively predicted the basic psychological needs and these predicted the intrinsic motivation, which in turn predicted group cohesion and satisfaction with life. The model describes the possible importance of promoting the teacher’s interpersonal style of autonomy support within the university setting in the search for satisfaction with life along with the active role of the student through the mediation of the satisfaction of basic psychological needs, increased quality motivation, and high group cohesion.
Development and Validation of a Questionnaire on Consumer Psychological Capital in Food Safety Social Co-governance
Authors: Meng Chun , Sun Lin , Guo Xiaoni , Wu Miao , Wang Yuqi , Yang Lingping , Peng Bin
Published: January 2021

Consumers play an important role as one of the main actors in food safety social co-governance. To create a pattern of food safety social co-governance, the active and effective participation of consumers is critical. To encourage consumers to participate in food safety social co-governance voluntarily and positively, we attempted to develop and preliminarily validate a multidimensional questionnaire on consumer psychological capital that could be used to measure the degree of consumer participation in food safety social co-governance. The aim of the initial sample (N = 170) and test sample 2 (N = 204) was to investigate the factor structure of a preliminary measure of consumer psychological capital. A 4-factor model with 23 items explained 61.05% of the total variance in item scores. The aim of test sample 3 (N = 30) was to measure the retest reliability. Test sample 4 (N = 1,076) was randomly allocated to the modeling sample (N = 538) and validation sample (N = 538) to verify questionnaire reliability and validity. Convergent validity, discriminant validity, and the internal inconsistency coefficients of the questionnaire were assessed in the modeling sample. While processing CFA, we deleted 9 items with small standardized factor loadings. The remaining 14 items in the final revised 4-factor model included self-efficacy, resilience, hope, and optimism. The fit indices of the revised four-factor model and second-order factor model in the modeling sample revealed an acceptable model fit. The convergent validity and discriminant validity of the revised model were good and acceptable, respectively. A cross-validation procedure confirmed the appropriateness of the revised four-factor model and second-order factor model in the validation sample. The cross-validation results confirmed that the fit indices of the revised four-factor model fitted the data well and the second-order factor model in the validation sample reached acceptable values. We concluded that the questionnaire developed in this study had good reliability and stable and acceptable construct validity. It could provide a theoretical basis for measuring psychological capital in food safety co-governance.
Relative Age Effect in Elite German Soccer: Influence of Gender and Competition Level
Authors: Götze Martin , Hoppe Matthias W.
Published: January 2021

The relative age effect (RAE) is associated with (dis)advantages in competitive sports. While the RAE in elite male soccer reveals a skewed birthdate distribution in relation to a certain cut-off date, research of RAE in elite female soccer is affected by small number of samples and conflicting results. The purpose of this study was to investigate the RAE in elite adult German soccer regarding gender and competition level. The sample comprised 680 female and 1,083 male players of the two top German leagues during the 2019/20 season and German national teams (A-Team to Under 19). Differences between the observed and expected birthdate distributions were analyzed using chi-square statistics and effect sizes followed by calculating odds ratios. Results showed a statistically significant RAE with small effect size across all players included for both genders (female players: P < 0.001, W = 0.16, male players: P < 0.001, W = 0.23). The identified RAE was based on an over-representation of players born at the beginning of the year. According to gender and competition level, RAEs were more pronounced in German male soccer. While significant RAEs were found among males in the first two leagues (first league: P < 0.001, W = 0.19, second league: P < 0.001, W = 0.26), the RAE of females was more pronounced in the second league (first league: P = 0.080, W = 0.16, second league: P = 0.002, W = 0.20). The analysis of RAE regarding the national teams revealed a statistically significant RAE with large effect size for only the youngest investigated age group of male players (Under 19: P = 0.022, W = 0.52). Our data show an RAE in female and male German adult soccer, which could be accompanied by a loss of valuable elite players during the youth phase of the career. Consequently, the pool of talented players at the adult level would be limited.
How Times of Crisis Serve as a Catalyst for Creative Action: An Agentic Perspective
Authors: Beghetto Ronald A.
Published: January 2021

The human experience is punctuated by times of crisis. Some crises are experienced at a personal level (e.g., the diagnosis of a life-threatening disease), organizational level (e.g., a business facing bankruptcy), and still others are experienced on a societal or global level (e.g., COVID-19 pandemic). Although crises can be deeply troubling and anxiety provoking, they can also serve as an important catalyst for creative action and innovative outcomes. This is because during times of crisis our typical forms of reasoning and action may no longer serve us. It is precisely during such times that new ways of thought, action and leadership are needed. A key question for researchers to consider is:Why and how times of crisis serve as an impetus for creative actions and outcomes?The purpose of this paper is to address this question. I open by briefly discussing the features of a crisis. I then introduce an empirically testable, process model that outlines various pathways, factors, and outcomes associated with different ways people and organizations respond during times of crisis. I close by briefly outlining future directions for creativity theory and research.
Changes in Catastrophic Health Expenditures Depending on Health Policies in Turkey
Authors: Kockaya Guvenc , Oguzhan Gülpembe , Çalşkan Zafer
Published: January 2021

Without any financial protection out of pocket health expenses are essential both because their increase causes difficulties in accessing higher quality health services for households and more importantly because it complicates access to most basic health services. As a result of the Health Transformation Program in practice in the Turkish healthcare system since 2003, significant changes have been done in all layers of the health system. Turkish Statistics Institute (TurkStat) publishes the ratio of households that bear catastrophic health expenditures since 2002. According to TurkStat data, the ratio of households with catastrophic expenditure has fallen from 0.81% in 2002 to 0.17% in 2011 with the health transformation project. However, it has started to rise since 2012 and has reached 0.31% in 2014. This study aims to evaluate the expenditure items that may have caused the rise of the ratio of households with catastrophic health expenditures since 2012, which had previously dropped with the Health Transformation Program that has caused fundamental changes in health policies. Methodology and definitions presented in the article named “Distribution of health payments and catastrophic expenditures: Methodology” by Ke Xu published by the World Health Organization in 2005 have been used. Percentages of health expenditure items among the total expenditure of households with positive health expenditure and households with catastrophic health expenditure between 2007 and 2014 have been evaluated using descriptive analysis. Findings have been interpreted in light of the health policies in practice between 2007 and 2014. An overview of the impact of the health policies reveals that medicine expenditures have decreased both for household and public health expenditures. Despite the impact of policies on the pharmaceutical industry was criticized by the industry, the positive impact can be seen by the decrease in the spending on medicine for households spending on health. Hospital service with positive health expenditure is seen to decrease health expenditure. The reasons for the increase in households with catastrophic health expenditure need further research. As a result, the study strives to discuss the possible policy reasons for the observed effects.
Relationship Between Muscular Activity and Assistance Magnitude for a Myoelectric Model Based Controlled Exosuit
Authors: Missiroli Francesco , Lotti Nicola , Xiloyannis Michele , Sloot Lizeth H. , Riener Robert , Masia Lorenzo
Published: January 2021

The growing field of soft wearable exosuits, is gradually gaining terrain and proposing new complementary solutions in assistive technology, with several advantages in terms of portability, kinematic transparency, ergonomics, and metabolic efficiency. Those are palatable benefits that can be exploited in several applications, ranging from strength and resistance augmentation in industrial scenarios, to assistance or rehabilitation for people with motor impairments. To be effective, however, an exosuit needs to synergistically work with the human and matching specific requirements in terms of both movements kinematics and dynamics: an accurate and timely intention-detection strategy is the paramount aspect which assume a fundamental importance for acceptance and usability of such technology. We previously proposed to tackle this challenge by means of a model-based myoelectric controller, treating the exosuit as an external muscular layer in parallel to the human biomechanics and as such, controlled by the same efferent motor commands of biological muscles. However, previous studies that used classical control methods, demonstrated that the level of device's intervention and effectiveness of task completion are not linearly related: therefore, using a newly implemented EMG-driven controller, we isolated and characterized the relationship between assistance magnitude and muscular benefits, with the goal to find a range of assistance which could make the controller versatile for both dynamic and static tasks. Ten healthy participants performed the experiment resembling functional daily activities living in separate assistance conditions: without the device's active support and with different levels of intervention by the exosuit. Higher assistance levels resulted in larger reductions in the activity of the muscles augmented by the suit actuation and a good performance in motion accuracy, despite involving a decrease of the movement velocities, with respect to the no assistance condition. Moreover, increasing torque magnitude by the exosuit resulted in a significant reduction in the biological torque at the elbow joint and in a progressive effective delay in the onset of muscular fatigue. Thus, contrarily to classical force and proportional myoelectric schemes, the implementation of an opportunely tailored EMG-driven model based controller affords to naturally match user's intention detection and provide an assistance level working symbiotically with the human biomechanics.