bluuebunny/crossref_metadata_2025 huggingface dataset:
Inflammation in the Peripheral Nervous System after Injury
Authors: Gu Dandan , Xia Yiming , Ding Zihan , Qian Jiaxi , Gu Xi , Bai Huiyuan , Jiang Maorong , Yao Dengbing
Published: June 2024

Nerve injury is a common condition that occurs as a result of trauma, iatrogenic injury, or long-lasting stimulation. Unlike the central nervous system (CNS), the peripheral nervous system (PNS) has a strong capacity for self-repair and regeneration. Peripheral nerve injury results in the degeneration of distal axons and myelin sheaths. Macrophages and Schwann cells (SCs) can phagocytose damaged cells. Wallerian degeneration (WD) makes the whole axon structure degenerate, creating a favorable regenerative environment for new axons. After nerve injury, macrophages, neutrophils and other cells are mobilized and recruited to the injury site to phagocytose necrotic cells and myelin debris. Pro-inflammatory and anti-inflammatory factors involved in the inflammatory response provide a favorable microenvironment for peripheral nerve regeneration and regulate the effects of inflammation on the body through relevant signaling pathways. Previously, inflammation was thought to be detrimental to the body, but further research has shown that appropriate inflammation promotes nerve regeneration, axon regeneration, and myelin formation. On the contrary, excessive inflammation can cause nerve tissue damage and pathological changes, and even lead to neurological diseases. Therefore, after nerve injury, various cells in the body interact with cytokines and chemokines to promote peripheral nerve repair and regeneration by inhibiting the negative effects of inflammation and harnessing the positive effects of inflammation in specific ways and at specific times. Understanding the interaction between neuroinflammation and nerve regeneration provides several therapeutic ideas to improve the inflammatory microenvironment and promote nerve regeneration.
Induction Therapies Determine the Distribution of Perforin and Granzyme B Transcripts in Kidney Transplant Recipients
Authors: Pipic Dino , Rasmussen Marianne , Saleh Qais W. , Tepel Martin
Published: June 2024

Peripheral blood mononuclear cells contain secretory granules with Perforin and Granzyme B for defense against pathogens. The objective of the present study was to compare the effects of immunosuppressive induction therapies on Perforin and Granzyme B transcripts in kidney transplant recipients. Transcripts were determined in 408 incident kidney transplant recipients eight days posttransplant using quantitative real-time PCR. Compared to 90 healthy subjects, the median Perforin transcripts were lower in kidney transplant recipients with blood-group ABO-incompatible donors (N = 52), compatible living donors (N = 130), and deceased donors (N = 226) (25.7%; IQR, 6.5% to 46.0%; 31.5%; IQR, 10.9% to 57.7%; and 35.6%; IQR, 20.6% to 60.2%; respectively; p = 0.015 by the Kruskal–Wallis test). Kidney transplant recipients who were treated with thymoglobulin (N = 64) had significantly lower Perforin as well as Granzyme B compared to all other induction therapies (N = 344) (each p < 0.001). Receiver operator characteristics analysis showed that both Perforin (area under curve, 0.919) and Granzyme B (area under curve, 0.915) indicated thyroglobulin-containing induction therapies. Regression analysis showed that both reduction in plasma creatinine and human leukocyte antigen mismatches were positively associated with elevated Perforin/Granzyme B transcript ratio posttransplant. We conclude clinical parameters and therapies affect Perforin and Granzyme B transcripts posttransplant.
Evaluation of PAGE-B Score for Hepatocellular Carcinoma Development in Chronic Hepatitis B Patients: Reliability, Validity, and Responsiveness
Authors: Tourkochristou Evanthia , Kalafateli Maria , Triantos Christos , Aggeletopoulou Ioanna
Published: June 2024

Chronic hepatitis B (CHB) constitutes a major global public health issue, affecting millions of individuals. Despite the implementation of robust vaccination programs, the hepatitis B virus (HBV) significantly influences morbidity and mortality rates. CHB emerges as one of the leading causes of hepatocellular carcinoma (HCC), introducing a major challenge in the effective management of CHB patients. Therefore, it is of utmost clinical importance to diligently monitor individuals with CHB who are at high risk of HCC development. While various prognostic scores have been developed for surveillance and screening purposes, their accuracy in predicting HCC risk may be limited, particularly in patients under treatment with nucleos(t)ide analogues. The PAGE-B model, incorporating age, gender, and platelet count, has exhibited remarkable accuracy, validity, and reliability in predicting HCC occurrence among CHB patients receiving HBV treatment. Its predictive performance stands out, whether considered independently or in comparison to alternative HCC risk scoring systems. Furthermore, the introduction of targeted adjustments to the calculation of the PAGE-B score might have the potential to further improve its predictive accuracy. This review aims to evaluate the efficacy of the PAGE-B score as a dependable tool for accurate prediction of the development of HCC in CHB patients. The evidence discussed aims to provide valuable insights for guiding recommendations on HCC surveillance within this specific population.
Kinetic Model of Fluorescein Release through Bioprinted Polylactic Acid Membrane
Authors: de Nigris Antonio , Minó Antonio , Cinelli Giuseppe , Colella Matilde , Lopez Francesco , Ambrosone Luigi
Published: June 2024

Polylactic acid (PLA)-based cylindrical membranes for the controlled release of fluorescein sodium salt (FS) were prepared by bioprinting on systems with an initial FS concentration of 0.003763 gdm−3 and 37.63 gdm−3, and the drug release process was monitored in a bath at 37 °C. Photographs, acquired at regular intervals during the process, revealed marked osmotic swelling of the polymer. Osmotic swelling consists in the enlargement of the polymer structure and due to the influx of water molecules across the membrane. The cylindrical PLA membrane starts to significantly swell once a certain threshold range is crossed. Important amounts of FS can dissolve under these radically changed circumstances, and the dissolved FS molecules are mobile enough to diffuse out of the cylinder, thus allowing drug release. As a matter of fact, in this investigation, we ascertained that polymer swelling promotes the mass transport phenomenon by altering the conditions for drug dissolution and diffusion, hence facilitating FS release after a specific lag time. Furthermore, in order to compare the release kinetics, the half-release time, t0.5, was taken into consideration. The data of this study evidence that, while increasing the initial concentration of FS by three orders of magnitude, the time parameter, t0.5, is only reduced by 5/6. In addition, the yield of the release process is drastically reduced due to the strong aggregation ability of the dye. Finally, it is demonstrated that a compressed exponential kinetic model fits the experimental data well despite the varying physical conditions.
Long-Term Detection of Glycemic Glucose/Hypoglycemia by Microfluidic Sweat Monitoring Patch
Authors: Xu Wenjie , Lu Lei , He Yuxin , Cheng Lin , Liu Aiping
Published: June 2024

A microfluidic sweat monitoring patch that collects human sweat for a long time is designed to achieve the effect of detecting the rise and fall of human sweat glucose over a long period of time by increasing the use time of a single patch. Five collection pools, four serpentine channels, and two different valves are provided. Among them, the three-dimensional valve has a large burst pressure as a balance between the internal and external air pressures of the patch. The bursting pressure of the two-dimensional diverter valve is smaller than that of the three-dimensional gas valve, and its role is to control the flow direction of the liquid. Through plasma hydrophilic treatment of different durations, the optimal hydrophilic duration is obtained. The embedded chromogenic disc detects the sweat glucose value at two adjacent time intervals and compares the information of the human body to increase or reduce glucose. The patch has good flexibility and can fit well with human skin, and because polydimethylsiloxane (PDMS) has good light transmission, it reduces the measurement error caused by the color-taking process and makes the detection results more accurate.
Flight Initiation Distance in an Urban Bird: Influence of the Number of People, Gaze Orientation, and Bird Behavior
Authors: de Resende Natália , Teixeira Camila , de Azevedo Cristiano
Published: June 2024

The flight initiation distance (FID) measures the distance a bird flees from an approaching predator. Factors such as the frequency of predator approaches, the direction of predator gaze, variations in predator presence across different areas, and the specific behaviors displayed by predators can all affect the FID. For birds, people can assume the role of predators. This study aimed to evaluate whether the FID of the species Sicalis flaveola (Saffron finch, Aves, Passeriformes) is influenced by the number of people and their gaze direction, comparing areas with the greater and lesser flow of people and relating to the types of behavior exhibited by the birds. The results showed that the number of people walking towards the bird influenced the escape behavior, with more people generating longer FIDs than fewer people. If the approach was with the eyes fixed on the birds, the FIDs were longer. When birds were alert, FIDs were longer. Overall, the findings imply that birds exhibit nuanced reactions to human presence, even in areas with frequent human encounters, highlighting their advanced capacity for assessing and responding to perceived risks.
The Role of Consciousness in Coupling Emotions, Motivations, and Behaviors
Authors: Frumento Sergio , Menicucci Danilo
Published: June 2024

A potential function of consciousness is to integrate emotions, motivations, and subsequent behaviors into a coherent narrative [...]
Assessing the Impact of Recommendation Novelty on Older Consumers: Older Does Not Always Mean the Avoidance of Innovative Products
Authors: Zhao Li , Fu Bing
Published: June 2024

Personalized recommendations that use digital technologies to predict user interests and preferences and give guiding conclusions have become a widely used digital marketing tool on e-commerce platforms. Given that existing consumer behavior research has not reached a consensus on the relationship between age and the adoption of innovative products, whether recommendation novelty can stimulate older consumers’ acceptance of innovative products remains uncertain. Grounded in the aging and social influence literature, this experimental study investigated the moderating role of individual cognitive age on the impact of recommendation novelty on consumer perceptions regarding stereotype threat and receptiveness to innovativeness. An experiment involving 239 online shoppers was conducted to investigate the experiences of cognitively younger and older adults while using low or high levels of recommendation novelty designed for this study. Results reveal the tension for older adults when using highly recommended novelty, as they perceive these to be more of a stereotype threat, but they also have a higher level of receptiveness to innovativeness. This finding is contrary to the common belief that “the older the consumer, the less receptive to innovativeness”, providing novel insight into the information systems literature. Theoretically, this research shows how increasing the level of recommended novelty affects stereotype threat and receptiveness to innovativeness (of consumers of different cognitive ages). For practitioners, the results provide important guidelines on the kind of personalized recommendations that are appropriate for consumers with different cognitive ages.
Examining Public Service Motivation’s Impact on Organizational Commitment: Focusing on Moderating Roles of Hygiene and Motivation Factors
Authors: Jung Geon , Moon Kuk-Kyoung
Published: June 2024

Despite previous studies on public service motivation among public sector employees, the empirical analysis of the influential contextual conditions that moderate public service motivation’s impact on employees’ work attitudes remains inadequate. Given these limitations, this study examines public service motivation’s effects on public employees’ organizational commitment and investigates how Herzberg’s hygiene and motivation factors moderate the relationship between these aspects in the context of South Korea’s central government. For this, we used the data of 1021 public employees from the Differences in the Values of Different Generations of Public Officials and Organizational Innovation Survey, which the Korea Institute of Public Administration had conducted in 2022. We analyzed the data through hierarchical multiple regression analyses, and the results indicated that public service motivation exerts a positive effect on organizational commitment. Specifically, hygiene factors weaken the positive relationship between public service motivation and organizational commitment, while motivation factors have a moderating effect that strengthens said relationship. These findings suggest that, in order to enhance organizational commitment among motivated public employees, human resource management practices should prioritize motivational factors that can enhance job content, meaning, and autonomy instead of solely focusing on providing monetary incentives or improving external conditions.
Seismic Isolation Performance of Nuclear Power Plant Containment Structures
Authors: Xue Zhicheng , Cui Xiujun , Pei Qiang , Zhong Jintu , He Yongyi , Zhang Yao
Published: June 2024

Non-isolated structures have strong destructive effects and poor isolation effects when encountering earthquakes. Setting isolation bearings can prolong the natural vibration period of the structure, reduce the horizontal seismic response of the structure under the influence of variables such as acceleration, base reaction, and inter story displacement, and enhance the overall seismic performance of the structure. The new material—epoxy plate thick layer rubber isolation bearing—has unique advantages compared to other bearings, such as effective energy absorption, simple construction, and low cost. This study establishes a three-dimensional isolated nuclear power plant containment structure based on the principle of similarity ratio, and compares and analyzes the acceleration, base reaction, and displacement responses of non-isolated and isolated structures. At the same time, the incremental dynamic analysis method (IDA) is used to analyze the seismic vulnerability of the structure, and the isolation performance of the nuclear containment structure using epoxy plate thick layer rubber isolation bearings is comprehensively and deeply explored. The results show that the epoxy plate thick layer rubber isolation bearing effectively prolongs the natural vibration period of the structure, reduces the horizontal seismic response of the structure, reduces the dome acceleration response by 66.55%, and reduces the base horizontal shear force by 55.51%. Therefore, setting epoxy plate thick layer rubber isolation bearings in the isolation layer can effectively enhance the seismic performance of the structure, thereby improving the redundancy of the nuclear power plant containment structure.
Numerical Study of the Ultimate Bearing Capacity of Two Adjacent Rough Strip Footings on Granular Soil: Effects of Rotational and Horizontal Constraints of Footings
Authors: Salari Mahdi , Lezgy-Nazargah Mojtaba , Shafaie Vahid , Movahedi Rad Majid
Published: June 2024

In this paper, the numerical study of the ultimate bearing capacity (UBC) of two closely spaced strip footings on granular soil is investigated using the finite element method (FEM) and upper bound limit analysis (UBLA). Although the UBC of two adjacent footings has previously been studied in other experimental and numerical research, in all the previously reported studies, the footings were not allowed to rotate and move horizontally freely. Due to the deformation of the soil medium, two closely spaced footings are subjected to horizontal movements and tilting, even under central vertical loads. When the two adjacent footings are not permitted to rotate and move in the horizontal directions, the unwanted bending moment and horizontal force act on the footings. Indeed, the UBC of two closely spaced rough footings is evaluated under incorrect constraints in earlier research. In the present research, the UBC of two adjacent rough footings is evaluated with and without these incorrect constraints. The key finding of this study is that constraining the horizontal and rotational movement of the foundation artificially increases the UBC, which does not reflect field conditions. When foundations are permitted to rotate and move horizontally, there is no increase in UBC; however, there is an increased risk of differential settlement and structural instability.
Optimizing Three-Dimensional Trade-Off Problem of Time–Cost–Quality over Multi-Mode Projects with Generalized Logic
Authors: Aminbakhsh Saman , Abdulsattar Abdulrahman M.
Published: June 2024

Clients typically tend to aim for reasonable prices, minimum possible makespans, and the best quality for the construction projects that they engage in. Evidently, weighing the available offers and coming up with an optimal decision can pose challenges for the decision makers. In this regard, the generation of a tool that helps decision makers strike a proper balance among the conflicting project objectives (i.e., time, cost, and quality) is imperative. To this end, this study proposes a method which assists in the selection of the best compromise choices among the options available for each of the project activities. In addition to the time and cost, the proposed method is designed to bring the quality aspect into the equation as well. To quantify the quality, a value referring to the weighted importance and performance of each activity is used. The proposed method is based on a modified multi-objective genetic algorithm (GA) that incorporates the domination concept for the selection of the best solutions out of the potential candidates. The GA-based method is capable of handling an unlimited number of precedence relationships for each activity, and above all, it is able to capture and unravel any type of logical relationship. This very feature significantly improves the practical relevance of this research, as the parallelization of activities is a common practice in real-life projects. Planners benefit from the various types of relationships (i.e., Start to Start, Start to Finish, Finish to Start, and Finish to Finish), and the concept of lag time frequently introduces parallelization into the network. Overlapped activities, in turn, help reduce the unwanted idle times and speed up the project significantly. Accordingly, in order to demonstrate the application and effectiveness of the proposed model, it has been used in the solution of four time–cost–quality (TCQ) trade-off problems, three of which are generated within the context of this paper. The practiced instances include a small benchmark TCQT problem with 18 activities taken from the literature in addition to more complex 29- and 63-activity TCQTPs produced herein based on benchmark time–cost trade-off problems. The performance of the presented approach is ultimately examined over a large-scale, real-case construction project with over four hundred activities and generalized logic in an unprecedented attempt to validate a model in the realm of TCQTPs. The successful results of the experiments reveal the effectiveness of the proposed model and corroborate the feasibility of its application by the planners amidst arduous decision-making processes.
A Phase II Trial of Bevacizumab in Patients with Recurrent/Progressive Solid Tumor Brain Metastases That Have Progressed Following Whole-Brain Radiation Therapy
Authors: Dixit Karan , Singer Lauren , Grimm Sean Aaron , Lukas Rimas V. , Schwartz Margaret A. , Rademaker Alfred , Zhang Hui , Kocherginsky Masha , Chernet Sofia , Sharp Laura , Nelson Valerie , Raizer Jeffrey J. , Kumthekar Priya
Published: June 2024

Patients with solid tumor brain metastases that progress after whole-brain radiation have limited options. This prospective trial investigated the efficacy, safety, and tolerability of bevacizumab as salvage therapy in this population. Eligible patients received bevacizumab 10 mg/kg intravenously every 2 weeks until progression. The primary endpoint was radiologic response using Response Assessment in Neuro-Oncology (RANO) criteria. The secondary endpoints were progression-free survival (PFS), overall survival (OS), duration of response, and safety. Quality of life (QOL) was studied using the Functional Assessment of Cancer Therapy-Brain (FACT-Br) scale. Twenty-seven patients were enrolled, with twenty-four having evaluable data for response. The majority of histologies (n = 21, 78%) were breast cancer. The remaining histologies were non-small-cell lung cancer (n = 4, 15%), neuroendocrine cancer (n = 1, 3%), and papillary fallopian serous adenocarcinoma (n = 1, 3%). Eighteen patients had radiologic response, with two patients demonstrating partial response (8.33%) and sixteen patients demonstrating stable disease (66.7%). The median duration of response was 203 days. PFS at 6 months was 46%, median PFS was 5.3 m, and median OS was 9.5 m. Treatment was well tolerated, with six patients experiencing grade 3 lymphopenia and hypertension. There was one grade 3 thromboembolism. QOL was not negatively impacted. Bevacizumab is a safe and feasible salvage treatment with durable response and favorable overall survival for patients with progressive brain metastases after whole-brain radiation.
Conditional Knockout of Neurexins Alters the Contribution of Calcium Channel Subtypes to Presynaptic Ca2+ Influx
Authors: Brockhaus Johannes , Kahl Iris , Ahmad Mohiuddin , Repetto Daniele , Reissner Carsten , Missler Markus
Published: June 2024

Presynaptic Ca2+ influx through voltage-gated Ca2+ channels (VGCCs) is a key signal for synaptic vesicle release. Synaptic neurexins can partially determine the strength of transmission by regulating VGCCs. However, it is unknown whether neurexins modulate Ca2+ influx via all VGCC subtypes similarly. Here, we performed live cell imaging of synaptic boutons from primary hippocampal neurons with a Ca2+ indicator. We used the expression of inactive and active Cre recombinase to compare control to conditional knockout neurons lacking either all or selected neurexin variants. We found that reduced total presynaptic Ca2+ transients caused by the deletion of all neurexins were primarily due to the reduced contribution of P/Q-type VGCCs. The deletion of neurexin1α alone also reduced the total presynaptic Ca2+ influx but increased Ca2+ influx via N-type VGCCs. Moreover, we tested whether the decrease in Ca2+ influx induced by activation of cannabinoid receptor 1 (CB1-receptor) is modulated by neurexins. Unlike earlier observations emphasizing a role for β-neurexins, we found that the decrease in presynaptic Ca2+ transients induced by CB1-receptor activation depended more strongly on the presence of α-neurexins in hippocampal neurons. Together, our results suggest that neurexins have unique roles in the modulation of presynaptic Ca2+ influx through VGCC subtypes and that different neurexin variants may affect specific VGCCs.
Increasing GSH-Px Activity and Activating Wnt Pathway Promote Fine Wool Growth in FGF5-Edited Sheep
Authors: Xu Xue-Ling , Wu Su-Jun , Qi Shi-Yu , Chen Ming-Ming , Liu Zhi-Mei , Zhang Rui , Zhao Yue , Liu Shun-Qi , Zhou Wen-Di , Zhang Jin-Long , Zhang Xiao-Sheng , Deng Shou-Long , Yu Kun , Li Yan , Lian Zheng-Xing
Published: June 2024

Fibroblast growth factor 5 (FGF5) plays key roles in promoting the transition from the anagen to catagen during the hair follicle cycle. The sheep serves as an excellent model for studying hair growth and is frequently utilized in various research processes related to human skin diseases. We used the CRISPR/Cas9 system to generate four FGF5-edited Dorper sheep and only low levels of FGF5 were detected in the edited sheep. The density of fine wool in GE sheep was markedly increased, and the proportion of fine wool with a diameter of 14.4–20.0 μm was significantly higher. The proliferation signal in the skin of gene-edited (GE) sheep was stronger than in wild-type (WT) sheep. FGF5 editing decreased cortisol concentration in the skin, further activated the activity of antioxidant enzymes such as Glutathione peroxidase (GSH-Px), and regulated the expression of Wnt signaling pathways containing Wnt agonists (Rspondins, Rspos) and antagonists (Notum) in hair regeneration. We suggest that FGF5 not only mediates the activation of antioxidant pathways by cortisol, which constitutes a highly coordinated microenvironment in hair follicle cells, but also influences key signals of the Wnt pathway to regulate secondary hair follicle (SHF) development. Overall, our findings here demonstrate that FGF5 plays a significant role in regulating SHF growth in sheep and potentially serves as a molecular marker of fine wool growth in sheep breeding.
Ileal Crohn’s Disease Exhibits Reduced Activity of Phospholipase C-β3-Dependent Wnt/β-Catenin Signaling Pathway
Authors: Ando Tomoaki , Takazawa Ikuo , Spencer Zachary T. , Ito Ryoji , Tomimori Yoshiaki , Mikulski Zbigniew , Matsumoto Kenji , Ishitani Tohru , Denson Lee A. , Kawakami Yu , Kawakami Yuko , Kitaura Jiro , Ahmed Yashi , Kawakami Toshiaki
Published: June 2024

Crohn’s disease is a chronic, debilitating, inflammatory bowel disease. Here, we report a critical role of phospholipase C-β3 (PLC-β3) in intestinal homeostasis. In PLC-β3-deficient mice, exposure to oral dextran sodium sulfate induced lethality and severe inflammation in the small intestine. The lethality was due to PLC-β3 deficiency in multiple non-hematopoietic cell types. PLC-β3 deficiency resulted in reduced Wnt/β-catenin signaling, which is essential for homeostasis and the regeneration of the intestinal epithelium. PLC-β3 regulated the Wnt/β-catenin pathway in small intestinal epithelial cells (IECs) at transcriptional, epigenetic, and, potentially, protein–protein interaction levels. PLC-β3-deficient IECs were unable to respond to stimulation by R-spondin 1, an enhancer of Wnt/β-catenin signaling. Reduced expression of PLC-β3 and its signature genes was found in biopsies of patients with ileal Crohn’s disease. PLC-β regulation of Wnt signaling was evolutionally conserved in Drosophila. Our data indicate that a reduction in PLC-β3-mediated Wnt/β-catenin signaling contributes to the pathogenesis of ileal Crohn’s disease.
Investigation of Variability of Flaw Strength Distributions on Brittle SiC Ceramic
Authors: Lamon Jacques
Published: June 2024

The present paper investigates flaw strength distributions established using various flexural tests on batches of SiC bar test specimens, namely four-point bending as well as three-point bending tests with different span lengths. Flaw strength is provided by the elemental stress operating on the critical flaw at the fracture of a test specimen. Fracture-inducing flaws and their locations are identified using fractography. A single population of pores was found to dominate the fracture. The construction of diagrams of p-quantile vs. elemental strengths was aimed at assessing the Gaussian nature of flaw strengths. Then, empirical cumulative distributions of strengths were constructed using the normal distribution function. The Weibull distributions of strengths are then compared to the normal reference distributions. The parameters of the Weibull cumulative probability distributions are estimated using maximum likelihood and moment methods. The cumulative distributions of flexural strengths for the different bending tests are predicted from the flaw strength density function using the elemental strength model, and from the cumulative distribution of flexural strength using the Weibull function. Flaw strength distributions that include the weaker flaws that are potentially present in larger test pieces are extrapolated using the p-quantile diagrams. Implications are discussed regarding the pertinence of an intrinsically representative flaw strength distribution, considering failure predictions. Finally, the influence of the characteristics of fracture-inducing flaw populations expressed in terms of flaw strength interval, size, dispersion, heterogeneity, and reproducibility with volume change is examined.
A Flow-Through Biosensor System Based on Pillar[3]Arene[2]Quinone and Ferrocene for Determination of Hydrogen Peroxide and Uric Acid
Authors: Stoikov Dmitry , Shafigullina Insiya , Shurpik Dmitry , Stoikov Ivan , Evtugyn Gennady
Published: June 2024

Simple and reliable electrochemical sensors are highly demanded in medicine and pharmacy for the fast determination of metabolites and biomarkers of diseases. In this work, a flow-through biosensor system was developed on the base of a screen-printed carbon electrode modified with pillar[3]arene[2]quinone and ferrocene implemented in carbon black. The modification was performed in a single step and resulted in the formation of a stable layer with good operation characteristics. Uricase was immobilized on the inner walls of a replaceable reactor by carbodiimide binding. A flow-through cell was manufactured by 3D printing from poly(lactic acid). The flow-through system was first optimized on the hydrogen peroxide assay and then used for the determination of 1 nM–0.1 mM uric acid (limit of detection 0.3 nM, 20 measurements per hour). Implementation of ferrocene resulted in a synergetic increase in the cathodic current of H2O2 reduction measured by flow switching in chronoamperometric mode. The developed system was tested on the determination of uric acid in artificial urine and Ringer–Locke solution and showed a recovery rate of 96–112%. In addition, the possibility of determination of H2O2 in commercial disinfectants was shown. Easy assembly, fast and reliable signal and low consumption of the reagents make the system developed attractive for routine clinical analysis of metabolites.
Interaction of the Polymeric Layer Derived from 3-(4-Trifluoromethyl)-phenyl)-thiophene with Synthetic Stimulants on the Phase Boundary
Authors: Shishkanova Tatiana V. , Štěpánková Natalie , Broncová Gabriela , Vrňata Martin
Published: June 2024

Modification of an electrode surface with a selective layer leads to amplification of the electrochemical signal. A film derived from electrochemically oxidized 3-(4-trifluoromethyl)-phenyl)-thiophene deposited on a graphite electrode (ThPhCF3/G) was used to estimate the affinity for synthetic stimulants (2-aminoindane, buphedrone, naphyrone) using a combination of square wave voltammetry and electrochemical impedance spectroscopy. The modified surface was characterized using Raman spectroscopy, which confirmed that the presence of the –PhCF3 group is important for the recognition of synthetic stimulants. The determined values of the adsorption constants (Kads) showed the significance of charge–transfer and/or hydrogen bond interactions between—PhCF3 groups in the polymeric film and the analyte of interest: buphedrone (9.79 × 105) < naphyrone (1.57 × 106) < 2-AI (1.87 × 106). Compared to electrodes modified with nanomaterial, PThPhCF3/G-electrodes showed the highest sensitivity in concentration range of 1–11 μmol L−1 at neutral pH and a possibility of detection of 0.43–0.56 μg mL−1 (sr = 0.05–0.12). The analytical performance of ThPhCF3/G promises good perspectives for the detection of synthetic stimulants in forensic samples without prior pretreatment.
Thermal Modulation of Resistance Gas Sensor Facilitates Recognition of Fragrance Odors
Authors: Sui Ran , Zhang Erpan , Tang Xiaoshui , Yan Wenjun , Liu Yun , Zhou Houpan
Published: June 2024

Herein, we prepared two different MOS-based gas sensors with integrated micro-hotplates. The two sensors were employed to detect various fragrances (cedar, mandarin orange, rose A, and rose B), exhibiting similarly great sensing performances. The gas sensing properties of the MOS-based sensor depend on the sensor’s operating temperature. In addition to isothermal operation, various pulse heating modes were applied to investigate the gas sensing performances with respect to the four fragrances. Multivariate gas sensing features of the four fragrances were obtained under different operating modes, which were utilized for the recognition of fragrance odors successfully, based on the long short-term memory (LSTM) algorithm.
Portable Sensing Platform for the Visual Detection of Iodide Ions in Food and Clinical Samples
Authors: Khan Aizaz , Jafry Ali Turab , Ajab Huma , Yaqub Asim , Jilani Shahaab , Hussain Dildar , Abbas Naseem
Published: June 2024

The detection of iodide ions (I−), despite challenges due to low concentrations and potential masking, is crucial for studying physiological processes and diagnosing diseases. A colorimetric sensor was developed to improve I− ion monitoring and facilitate on-site detection based on filter paper, which is a cost-effective platform. The sensor observed color changes in response to the exposure of hydrogen peroxide (H2O2), 3,3′,5,5′-tetramethylbenzidine (TMB), from colorless to yellowish brown. The sensor demonstrated a detection limit of 0.125 × 10−6 M for I− ions in a relatively wide range of 0.01 to 15 × 10−6 M under optimized conditions including gel concentration, temperature, incubation time, TMB and H2O2 concentration, and pH. Furthermore, the proposed sensor was successfully employed in a variety of applications, such as biological (urine and blood serum), food (egg yolk and snacks), and environmental samples (tap water). The study established effective recoveries in complex media for visual on-site I− ion monitoring, indicating the developed assay as a potent, affordable, and practical platform.
Multiomics Analysis of the PHLDA Gene Family in Different Cancers and Their Clinical Prognostic Value
Authors: Iqbal Safia , Karim Md. Rezaul , Mohammad Shahnawaz , Mathiyalagan Ramya , Morshed Md. Niaj , Yang Deok-Chun , Bae Hyocheol , Rupa Esrat Jahan , Yang Dong Uk
Published: June 2024

The PHLDA (pleckstrin homology-like domain family) gene family is popularly known as a potential biomarker for cancer identification, and members of the PHLDA family have become considered potentially viable targets for cancer treatments. The PHLDA gene family consists of PHLDA1, PHLDA2, and PHLDA3. The predictive significance of PHLDA genes in cancer remains unclear. To determine the role of pleckstrin as a prognostic biomarker in human cancers, we conducted a systematic multiomics investigation. Through various survival analyses, pleckstrin expression was evaluated, and their predictive significance in human tumors was discovered using a variety of online platforms. By analyzing the protein–protein interactions, we also chose a collection of well-known functional protein partners for pleckstrin. Investigations were also carried out on the relationship between pleckstrins and other cancers regarding mutations and copy number alterations. The cumulative impact of pleckstrin and their associated genes on various cancers, Gene Ontology (GO), and pathway analyses were used for their evaluation. Thus, the expression profiles of PHLDA family members and their prognosis in various cancers may be revealed by this study. During this multiomics analysis, we found that among the PHLDA family, PHLDA1 may be a therapeutic target for several cancers, including kidney, colon, and brain cancer, while PHLDA2 can be a therapeutic target for cancers of the colon, esophagus, and pancreas. Additionally, PHLDA3 may be a useful therapeutic target for ovarian, renal, and gastric cancer.
Glutathione in HIV-Associated Neurocognitive Disorders
Authors: Erdos Thomas , Masuda Mika , Venketaraman Vishwanath
Published: June 2024

A large portion of patients with Human Immunodeficiency Virus (HIV) have neurologic sequelae. Those with better-controlled HIV via antiretroviral therapies generally have less severe neurologic symptoms. However, for many patients, antiretrovirals do not adequately resolve symptoms. Since much of the pathogenesis of HIV/AIDS (Autoimmune Deficiency Syndrome) involves oxidative stress either directly, through viral interaction, or indirectly, through inflammatory mechanisms, we have reviewed relevant trials of glutathione supplementation in each of the HIV-associated neurocognitive diseases and have found disease-specific results. For diseases for which trials have not been completed, predicted responses to glutathione supplementation are made based on relevant mechanisms seen in the literature. It is not sufficient to conclude that all HIV-associated neurocognitive disorders (HAND) will benefit from the antioxidant effects of glutathione supplementation. The potential effects of glutathione supplementation in patients with HAND are likely to differ based on the specific HIV-associated neurocognitive disease.
RETRACTED: Syah et al. The Effect of Structural Phase Transitions on Electronic and Optical Properties of CsPbI3 Pure Inorganic Perovskites. Coatings 2021, 11, 1173
Authors: Syah Rahmad , Davarpanah Afshin , Nasution Mahyuddin K. M. , Wali Qamar , Ramdan Dadan , Albaqami Munirah D. , Ouladsmane Mohamed , Noori Saja Mohammed
Published: June 2024

The Coatings Editorial Office retracts the article entitled “The Effect of Structural Phase Transitions on Electronic and Optical Properties of CsPbI3 Pure Inorganic Perovskites” [...]
Searching Questions and Learning Problems in Large Problem Banks: Constructing Tests and Assignments on the Fly
Authors: Sychev Oleg
Published: June 2024

Modern advances in creating shared banks of learning problems and automatic question and problem generation have led to the creation of large question banks in which human teachers cannot view every question. These questions are classified according to the knowledge necessary to solve them and the question difficulties. Constructing tests and assignments on the fly at the teacher’s request eliminates the possibility of cheating by sharing solutions because each student receives a unique set of questions. However, the random generation of predictable and effective assignments from a set of problems is a non-trivial task. In this article, an algorithm for generating assignments based on teachers’ requests for their content is proposed. The algorithm is evaluated on a bank of expression-evaluation questions containing more than 5000 questions. The evaluation shows that the proposed algorithm can guarantee the minimum expected number of target concepts (rules) in an exercise with any settings. The available bank and exercise difficulty chiefly determine the difficulty of the found questions. It almost does not depend on the number of target concepts per item in the exercise: teaching more rules is achieved by rotating them among the exercise items on lower difficulty settings. An ablation study show that all the principal components of the algorithm contribute to its performance. The proposed algorithm can be used to reliably generate individual exercises from large, automatically generated question banks according to teachers’ requests, which is important in massive open online courses.
Disturbances in the Skin Homeostasis: Wound Healing, an Undefined Process
Authors: Férnandez-Guarino Montserrat , Naharro-Rodriguez Jorge , Bacci Stefano
Published: June 2024

This review was written with the aim of examining the effects that cause an insult, such as a wound, to an organ, such as the skin. Before examining the cellular mechanisms relating to wound healing, the reader is invited to read about the structure of the skin as a necessary basis for understanding the final aim of this review. The structure of the skin as a basis for understanding the phenomena relating to wound healing is addressed, taking into account the updated literature that addresses the numerous problems of the skin microenvironment. Starting from this awareness, the paragraphs dedicated to wound healing become complicated when this phenomenon is not implemented and therefore while the problems of chronic wounds, keloids, and hypertrophic scars are addressed, these are pathologies that are still difficult to understand and treat today.
Oncologists’ Satisfaction with Virtual Care: A Questionnaire
Authors: Balitsky Amaris Karin , Cantor Nathan , Zhang Karen , Pond Greg , Levine Mark Norman
Published: June 2024

Introduction: Although virtual care (VC) has become an integral part of oncology care and healthcare delivery, clinicians’ perspectives on and satisfaction with this modality are not well understood. Methods: Using a National Network Forum framework and expert panel review, we developed a questionnaire to measure oncologists’ satisfaction with VC. The questionnaire was distributed to Canadian oncologists through medical society email lists (n = 1541). We used a 5-point Likert scale to capture their responses, which included strongly disagree (1), disagree (2), undecided (3), agree (4), and strongly agree (5). Results: A total of 61 oncologists and/or oncology trainees, of 768 (7.9%) who opened their email, completed questionnaires between October 2022 and January 2023. Every questionnaire item had a response rate greater than 98%. Seventy-two percent of the respondents were satisfied with VC. Oncologists who were less comfortable with technology were more likely to report lower levels of satisfaction (p < 0.001, Wilcoxon rank-sum). The questionnaire items that received the highest levels of agreement were related to VC reducing costs and improving access for patients and concerns about missing a diagnosis and assessing patients’ functional status. The questionnaire items that received the greatest disagreement were related to VC improving access for patients with language barriers, VC being associated with time-savings for clinicians, improvements in clinical efficacy, and more readily available lab tests. Conclusions: Most of the oncologists surveyed are satisfied with VC; however, there are some concerns with VC that need to be addressed. Future research on optimizing VC should address clinicians’ concerns, in addition to addressing the patient experience.
Giant Morpheaform Basal Cell Carcinoma Mimicking Scarring Alopecia: Exception Prone to Neglect
Authors: Tomasini Carlo Francesco , Fiandrino Giacomo , Favale Emanuele Mario , Antoci Francesca , Barruscotti Stefania
Published: June 2024

A 74-year-old woman in good general health presented with a 5-year history of progressive hair loss over several years, interpreted as female androgenetic alopecia (AGA), and was treated with topical 5% Minoxidil without improvement. The patient’s relevant medical history revealed infiltrating, triple-negative apocrine carcinoma of the right breast four years before, treated by quadrantectomy, radiation, lymphadenectomy and chemotherapy, with no recurrence at the last follow-up. On examination, there was an asymptomatic 15 × 15 cm firm and whitish area of scarring alopecia on the central scalp. Dermoscopy revealed multiple arborizing vessels and many telangiectasia. The clinical considerations included mainly cutaneous metastasis of breast carcinoma (alopecia neoplastica), pseudopelade of Broque and morpheaform basal cell carcinoma (BCC). A histopathologic examination revealed characteristic changes of morpheaform BCC with basaloid islands and cords of atypical basaloid cells diffusely infiltrating the dermis, embedded in a sclerotic and hypervascularized stroma. Secondary alopecia neoplastica due to morpheaform BCC on the scalp is an exceedingly rare entity, possessing subtle clinical features that may mimic both scarring and non-scarring alopecia. Delayed recognition may contribute to aggressive behavior and extensive local destruction. Treatment with hedgehog inhibitors in locally advanced BCC of the scalp, both in adjuvant and neoadjuvant modalities, is promising.
State Control Design of Ostensible Metzler Linear Systems with Unsigned Input Parameters
Authors: Krokavec Dušan , Filasová Anna
Published: June 2024

This paper deals with the design of a complete state control for input unsigned, rank deficient matrix parameters of a linear system with system dynamics defined by ostensible structures of Metzler matrices. The proposed solution is based on the principle of diagonal stabilization of positive systems and uses a stabilizing additional component over the decomposition of the Metzler matrix in solving the incomplete internal positivity of such linear system structures. The novelty of the proposed approach is the unified representation of the parametric constraints of the Metzler matrix and the structurally constrained system inputs using linear matrix inequalities, which guarantees that the closed-loop system will be asymptotically stable. Despite the complexity of the constraint conditions on this class of linear continuous systems, the design conditions are formulated using sharp linear matrix inequalities only. A detailed design process is presented using a system-linearized mathematical model to verify the superiority and practicality of the proposed method.
The Utility of Annual Reassessment of the International Working Group on the Diabetic Foot Diabetes-Related Foot Ulcer Risk Classification in the Primary Care Setting—A Cohort Study
Authors: Monteiro-Soares Matilde , Dores José , Alves-Palma Cristina , Galrito Susana , Ferreira-Santos Daniela
Published: June 2024

Background: We assessed the pertinence of updating the International Working Group on the Diabetic Foot (IWGDF) risk classification yearly in people with diabetes by quantifying the changes in the risk group and its accuracy in identifying those developing an ulcer (DFU) in a primary care setting. Methods: In our retrospective cohort study, we included all people with diabetes with a foot assessment registry between January 2016 and December 2018 in the Baixo Alentejo Local Health Unit. Foot-related data were collected at baseline after one and two years. DFU and/or death until December 2019 were registered. The proportion of people changing their risk status each year was calculated. Accuracy measures of the IWGDF classification to predict DFU occurrence at one, two, and three years were calculated. Results: A total of 2097 people were followed for three years, during which 0.1% died and 12.4% developed a DFU. After two years, 3.6% of the participants had progressed to a higher-risk group. The IWGDF classification presented specificity values superior to 90% and negative predictive values superior to 99%. Conclusion: Foot risk status can be safely updated every two years instead of yearly, mainly for those at very low risk. The IWGDF classification can accurately identify those not at risk of DFU.
A Comprehensive Review on Alcohol Abuse Disorder Fatality, from Alcohol Binges to Alcoholic Cardiomyopathy
Authors: Argo Antonina , Pitingaro Walter , Puntarello Maria , Buscemi Roberto , Malta Ginevra , D’Anna Tommaso , Albano Giuseppe Davide , Zerbo Stefania
Published: June 2024

Frequent and excessive consumption of alcohol, be it episodic or sustained misuse, ranks among the top causes of mortality globally. This comprehensive analysis seeks to elucidate how alcohol misuse precipitates death, with a particular focus on associated cardiac anomalies. Notably, the phenomenon of “Holiday Heart Syndrome”, linked to binge drinking, is recognized for inducing potentially fatal cardiac arrhythmias. Moreover, persistent alcohol consumption is implicated in the development of alcoholic cardiomyopathy, a condition that underlies heart failure and arrhythmic disturbances of the heart. Additionally, individuals undergoing withdrawal from alcohol frequently exhibit disruptions in normal heart rhythm, posing a risk of death. This review further delves into additional alcohol-related mortality factors, including the heightened likelihood of hypertension, cerebrovascular accidents (strokes), and the connection between excessive alcohol use and Takotsubo syndrome.