bluuebunny/crossref_metadata_2025 huggingface dataset:
Functions of RNA-Binding Proteins in Cardiovascular Disease
Authors: Ruffenach Grégoire , Medzikovic Lejla , Sun Wasila , Hong Jason , Eghbali Mansoureh
Published: December 2023

Gene expression is under tight regulation from the chromatin structure that regulates gene accessibility by the transcription machinery to protein degradation. At the transcript level, this regulation falls on RNA-binding proteins (RBPs). RBPs are a large and diverse class of proteins involved in all aspects of a transcript’s lifecycle: splicing and maturation, localization, stability, and translation. In the past few years, our understanding of the role of RBPs in cardiovascular diseases has expanded. Here, we discuss the general structure and function of RBPs and the latest discoveries of their role in pulmonary and systemic cardiovascular diseases.
Blood-Derived Lipid and Metabolite Biomarkers in Cardiovascular Research from Clinical Studies: A Recent Update
Authors: Kale Dipali , Fatangare Amol , Phapale Prasad , Sickmann Albert
Published: December 2023

The primary prevention, early detection, and treatment of cardiovascular disease (CVD) have been long-standing scientific research goals worldwide. In the past decades, traditional blood lipid profiles have been routinely used in clinical practice to estimate the risk of CVDs such as atherosclerotic cardiovascular disease (ASCVD) and as treatment targets for the primary prevention of adverse cardiac events. These blood lipid panel tests often fail to fully predict all CVD risks and thus need to be improved. A comprehensive analysis of molecular species of lipids and metabolites (defined as lipidomics and metabolomics, respectively) can provide molecular insights into the pathophysiology of the disease and could serve as diagnostic and prognostic indicators of disease. Mass spectrometry (MS) and nuclear magnetic resonance (NMR)-based lipidomics and metabolomics analysis have been increasingly used to study the metabolic changes that occur during CVD pathogenesis. In this review, we provide an overview of various MS-based platforms and approaches that are commonly used in lipidomics and metabolomics workflows. This review summarizes the lipids and metabolites in human plasma/serum that have recently (from 2018 to December 2022) been identified as promising CVD biomarkers. In addition, this review describes the potential pathophysiological mechanisms associated with candidate CVD biomarkers. Future studies focused on these potential biomarkers and pathways will provide mechanistic clues of CVD pathogenesis and thus help with the risk assessment, diagnosis, and treatment of CVD.
Marker Development and Pyramiding of Fhb1 and Fhb7 for Enhanced Resistance to Fusarium Head Blight in Soft Red Winter Wheat
Authors: Gyawali Binod , Scofield Steven R. , Mohammadi Mohsen
Published: December 2023

Fusarium head blight (FHB) is a devastating fungal disease of hexaploid wheat (Triticum aestivum). Several genetic loci were previously identified that control FHB resistance in wheat, including Fhb1. Fhb7, a major QTL conferring resistance to FHB, controlling for mycotoxin deoxynivalenol (DON) production, has been introgressed into soft red winter wheat (SRWW). As an exotic QTL, Fhb7 is associated with linkage drag, affecting agronomic and end-use quality performance. This study outlines a breeding strategy for introducing and pyramiding Fhb7 into SRWW breeding populations that already possessed Fhb1 and harbored some additional disease-resistance genes. In addition to the Fhb1-Fhb7 pyramiding, we developed gene-based markers for both genes and examined them on 57 SRWW breeding lines. Our data showed that 15 out of 57 breeding lines possessed both Fhb1 and Fhb7 resistant alleles. Two years of phenotypic data from the inoculated and misted irrigation field showed that the combination of Fhb1-Fhb7 lowers mycotoxin DON accumulation in kernels, which provides protection for end-users and the milling industry. The Fhb gene-pyramided lines, with the additional regionally important disease resistance genes, produced in this breeding pipeline showed reasonable agronomic traits and can be used in crossing programs for the widespread introgression in elite wheat cultivars.
Risk Factors for Nodal Failure in Patients with FIGO IIIC Cervical Cancer Receiving Definitive Image-Guided Radiotherapy
Authors: Liu Xiaoliang , Hou Xiaorong , Hu Ke , Zhang Fuquan , Wang Weiping , Ren Kang
Published: December 2023

Background: Nodal failure is a major failure pattern for patients with FIGO IIIC cervical cancer, which is further associated with worse survival. This study was designed to investigate risk factors for nodal failure in FIGO IIIC cervical cancer patients. Methods: The characteristics of positive lymph nodes (LNs) and relevant clinical factors of 162 FIGO IIIC cervical cancer patients were collected. The chi-square test and logistic regression model were used to identify risk factors for nodal failure. Results: In total, 368 positive LNs were identified, including 307 pelvic LNs and 61 para-aortic LNs. The nodal failure rates for all LNs, pelvic LNs, and para-aortic LNs were 9.2%, 7.8%, and 16.4%, respectively. After 20 fractions of RT, a nodal short diameter (D20F) ≥ 0.95 cm and a ratio of nodal shrinkage (ΔV20F) < 0.435 resulted; <4 cycles of chemotherapy indicated higher nodal failure rates for all LNs. For pelvic LNs, ΔV20F < 0.435 and <4 cycles of chemotherapy were associated with a higher incidence of nodal failure. For para-aortic LNs, ΔV20F < 0.435 was the only risk factor for nodal failure. Conclusions: Para-aortic LNs were more likely to experience nodal failure than pelvic LNs. Nodal shrinkage during radiotherapy and cycles of chemotherapy were associated with nodal failure in patients with FIGO IIIC cervical cancer.
Presumed Onchocerciasis Chorioretinitis Spilling over into North America, Europe and Middle East
Authors: Mansour Ahmad , Rodriguez Linnet , Mansour Hana , Yehia Madeleine , Battaglia Parodi Maurizio
Published: December 2023

Background: Newer generation ophthalmologists practicing in the developed world are not very familiar with some tropical ocular diseases due to the absence of reports in the ophthalmic literature over the past thirty years. Because of world globalization or due to influx of immigrants from sub-Saharan Africa, exotic retinal diseases are being encountered more often in ophthalmology clinics. Methods: A multicenter case series of chorioretinitis or optic neuritis with obscure etiology that used serial multimodal imaging. Results: Four cases qualified with the diagnosis of presumed ocular onchocerciasis based on their residence near fast rivers in endemic areas, multimodal imaging, long term follow-up showing progressive disease and negative workup for other diseases. Characteristic findings include peripapillary choroiditis with optic neuritis or atrophy, subretinal tracts of the microfilaria, progressive RPE atrophy around heavily pigmented multifocal chorioretinal lesions of varying shapes, subretinal white or crystalline dots, and response to ivermectin. Typical skin findings are often absent in such patients with chorioretinitis rendering the diagnosis more challenging. Conclusions: Familiarity with the myriad ocular findings of onchocerciasis, and a high-degree of suspicion in subjects residing in endemic areas can help in the correct diagnosis and implementation of appropriate therapy. Onchocercal chorioretinitis is a slow, insidious, progressive, and prolonged polymorphous disease.
Prediction of Postnatal Growth Failure in Very Low Birth Weight Infants Using a Machine Learning Model
Authors: Yoon So Jin , Kim Donghyun , Park Sook Hyun , Han Jung Ho , Lim Joohee , Shin Jeong Eun , Eun Ho Seon , Lee Soon Min , Park Min Soo
Published: December 2023

Accurate prediction of postnatal growth failure (PGF) can be beneficial for early intervention and prevention. We aimed to develop a machine learning model to predict PGF at discharge among very low birth weight (VLBW) infants using extreme gradient boosting. A total of 729 VLBW infants, born between 2013 and 2017 in four hospitals, were included. PGF was defined as a decrease in z-score between birth and discharge that was greater than 1.28. Feature selection and addition were performed to improve the accuracy of prediction at four different time points, including 0, 7, 14, and 28 days after birth. A total of 12 features with high contribution at all time points by feature importance were decided upon, and good performance was shown as an area under the receiver operating characteristic curve (AUROC) of 0.78 at 7 days. After adding weight change to the 12 features—which included sex, gestational age, birth weight, small for gestational age, maternal hypertension, respiratory distress syndrome, duration of invasive ventilation, duration of non-invasive ventilation, patent ductus arteriosus, sepsis, use of parenteral nutrition, and reach at full enteral nutrition—the AUROC at 7 days after birth was shown as 0.84. Our prediction model for PGF performed well at early detection. Its potential clinical application as a supplemental tool could be helpful for reducing PGF and improving child health.
Multi-Objective Co-Operative Game-Based Optimization for Park-Level Integrated Energy System Based on Exergy-Economic Analysis
Authors: Mo Lili , Deng Zeyu , Chen Haoyong , Lan Junkun
Published: December 2023

The park-level integrated energy system (PIES) can realize the gradient utilization of energy and improve the efficiency of energy utilization through the coupling between multiple types of energy sub-networks. However, energy analysis and exergy analysis cannot be used to evaluate the economics of PIES. In addition, conflicts of interest among integrated energy suppliers make the economic scheduling of the PIES more difficult. In this paper, we propose a multi-objective collaborative game-based optimization method based on exergy economics, in which the introduction of exergy economics realizes the economic assessment of any link within the PIES, and the optimization model constructed based on the potential game solves the problem of conflict of interest among multiple energy suppliers and improves the benefits of each supplier. Finally, taking a PIES in Guangzhou as an example, the rationality of the optimization scheme proposed in this paper is demonstrated by comparing it with the classical optimization scheme.
Control of Cement Timing, Mineralogy, and Texture on Hydro-chemo-mechanical Coupling from CO2 Injection into Sandstone: A Synthesis
Authors: Wu Zhidi , Simmons Jason D. , Otu Samuel , Rinehart Alex , Luhmann Andrew , Heath Jason , Mozley Peter , Majumdar Bhaskar S.
Published: December 2023

Carbon capture, utilization, and storage (CCUS) has been widely applied to enhance oil recovery (CO2-EOR). A thorough investigation of the impact of injecting CO2 into a heterogeneous reservoir is critical to understanding the overall reservoir robustness and storage performance. We conducted fifteen flow-through tests on Morrow B sandstone that allowed for chemical reactions between a CO2-rich brackish solution and the sandstones, and four creep/flow-through tests that simultaneously allowed for chemical reactions and stress monitoring. From fluid chemistry and X-ray computed tomography, we found that the dissolution of disseminated cements and the precipitation of iron-rich clays did not significantly affect the permeability and geomechanical properties. Minor changes in mechanical properties from Brazilian and creep tests indicated that the matrix structure was well-supported by early diagenetic quartz overgrowth cement and the reservoir’s compaction history at deep burial depths. However, one sample experienced a dissolution of poikilotopic calcite, leading to a permeability increase and significant tensile strength degradation due to pore opening, which overcame the effect of the early diagenetic cements. We concluded that the Morrow B sandstone reservoir is robust for CO2 injection. Most importantly, cement timing, the abundance and texture of reactive minerals, and the reservoir’s burial history are critical in predicting reservoir robustness and storage capacity for CO2 injection.
A Review on Solar Panel Cleaning Systems and Techniques
Authors: Najmi Nouhaila , Rachid Ahmed
Published: December 2023

This paper provides an overview of the cleaning aspects of solar panels through a literature review. We first discuss the drawbacks of unwanted deposits on solar panels in terms of energy production and efficiency. Existing cleaning practices and technologies are then presented with an emphasis on factors such as the size of the facility, location, cost, and available resources. Finally, a comparative cost–benefit analysis is carried out using decision support tools and taking into account different relevant criteria to support users choose the right cleaning maintenance for their specific solar installation.
Extreme Gradient Boosting Model for Day-Ahead STLF in National Level Power System: Estonia Case Study
Authors: Zhao Qinghe , Liu Xinyi , Fang Junlong
Published: December 2023

Short-term power load forecasting refers to the use of load and weather information to forecast the Day-ahead load, which is very important for power dispatch and the establishment of the power spot market. In this manuscript, a comprehensive study on the frame of input data for electricity load forecasting is proposed based on the extreme gradient boosting algorithm. Periodicity was the first of the historical load data to be analyzed using discrete Fourier transform, autocorrelation function, and partial autocorrelation function to determine the key width of a sliding window for an optimization load feature. The mean absolute error (MAE) of the frame reached 52.04 using a boosting model with a 7-day width in the validation dataset. Second, the fusing of datetime variables and meteorological information factors was discussed in detail and determined how to best improve performance. The datetime variables were determined as a form of integer, sine–cosine pairs, and Boolean-type combinations, and the meteorological features were determined as a combination with 540 features from 15 sampled sites, which further decreased MAE to 44.32 in the validation dataset. Last, a training method for day-ahead forecasting was proposed to combine the Minkowski distance to determine the historical span. Under this framework, the performance has been significantly improved without any tuning for the boosting algorithm. The proposed method further decreased MAE to 37.84. Finally, the effectiveness of the proposed method is evaluated using a 200-day load dataset from the Estonian grid. The achieved MAE of 41.69 outperforms other baseline models, with MAE ranging from 65.03 to 104.05. This represents a significant improvement of 35.89% over the method currently employed by the European Network of Transmission System Operators for Electricity (ENTSO-E). The robustness of the proposal method can be also guaranteed with excellent performance in extreme weather and on special days.
Changes in the Number of Vascular Plant Species during Reforestation of Clearcut Forests
Authors: Kryshen Alexander , Genikova Nadezhda
Published: December 2023

The article aims to describe the patterns of changes in the number of vascular plant species in communities at different stages of forest regeneration after clearcutting using the space-for-time substitution method. Large-scale logging in boreal forests leads to a simplification of the territory’s forest cover (the formation of even-aged stands), which in turn leads to a decrease in the diversity of forest communities and species diversity. These tendencies have been confirmed for many groups of organisms, especially those associated with old trees and dead wood; however, this is not so clear for vascular plants. We investigated plant communities at different stages of regeneration. We used the so-called space-for-time substitution (chronosequence) method of building dynamic series for four forest ecotopes, where industrial clearcuts are most actively carried out. We showed that the fertility of forest ecotopes determines the species pool—the richer the conditions, the more species that can potentially inhabit the community. At the same time, the structure of the tree stand significantly affects the composition and number of species in the community. The lowest species richness was in old-growth forests characterized by the dominance of one species in the tree stand and one or two vascular plant species in the ground cover. With insignificant fluctuations in the average number of species between stages of forest regeneration after logging, some communities at clearcuts in bilberry (Vaccinium myrtillus L.) pine (Pinus sylvestris L.) and spruce (Picea abies (L.) H.Karst.) forests have high species diversity due to the occupation of the site by species of open habitats. These clearcuts are located not far from settlements and close to roads.
Potential Effects of the Different Matrices to Enhance the Polyphenolic Content and Antioxidant Activity in Gluten-Free Bread
Authors: Bueno Carolina , Thys Roberta , Tischer Bruna
Published: December 2023

Gluten-related disorders, including celiac disease, wheat allergy, and non-celiac gluten sensitivity, have emerged as a significant phenomenon affecting people worldwide, with an estimated prevalence of nearly 5% globally. The only currently available treatment for this disease involves the exclusion of gluten from the diet, which is particularly challenging in the case of bakery products. Gluten-free bread (GFB) presents certain disadvantages when compared to traditional wheat bread, including inferior sensory attributes, technological characteristics, and lower protein and fiber content. Numerous studies have focused on strategies to improve these aspects of GFB. However, there are limited reviews regarding the content of the bioactive compounds of GFB, such as polyphenols. Polyphenols are molecules found in various foods that play a vital role in protecting the body against oxidative stress. This is particularly relevant for individuals with gluten intolerance or celiac disease, as they often experience increased oxidative stress and inflammation. Therefore, the objective of this review is to explore the use of different strategies for increasing the polyphenolic content and the antioxidant properties of GFB. Gluten-free cereals and pseudocereals are the most used matrices in GFB. Buckwheat can be a valuable matrix to enhance the nutritional profile and antioxidant properties of GFB, even more so when the whole grain is used. In the same way, the addition of various by-products can effectively increase the bioactive compounds and antioxidant activity of GFB. Furthermore, regarding the contribution of the phenolics to the bitterness, astringency, color, flavor, and odor of food, it is essential to analyze the sensory properties of these breads to ensure not only enriched in bioactive compounds, but also good consumer acceptance. In vitro studies are still in few number and are very important to execute to provide a better understanding of the bioactive compounds after their consumption.
Facile Fabrication of NIR-Responsive Alginate/CMC Hydrogels Derived through IEDDA Click Chemistry for Photothermal–Photodynamic Anti-Tumor Therapy
Authors: Rizwan Ali , Ali Israr , Jo Sung-Han , Vu Trung Thang , Gal Yeong-Soon , Kim Yong Hyun , Park Sang-Hyug , Lim Kwon Taek
Published: December 2023

Novel chemically cross-linked hydrogels derived from carboxymethyl cellulose (CMC) and alginate (Alg) were prepared through the utilization of the norbornene (Nb)–methyl tetrazine (mTz) click reaction. The hydrogels were designed to generate reactive oxygen species (ROS) from an NIR dye, indocyanine green (ICG), for combined photothermal and photodynamic therapy (PTT/PDT). The cross-linking reaction between Nb and mTz moieties occurred via an inverse electron-demand Diels–Alder chemistry under physiological conditions avoiding the need for a catalyst. The resulting hydrogels exhibited viscoelastic properties (G′ ~ 492–270 Pa) and high porosity. The hydrogels were found to be injectable with tunable mechanical characteristics. The ROS production from the ICG-encapsulated hydrogels was confirmed by DPBF assays, indicating a photodynamic effect (with NIR irradiation at 1–2 W for 5–15 min). The temperature of the ICG-loaded hydrogels also increased upon the NIR irradiation to eradicate tumor cells photothermally. In vitro cytocompatibility assessments revealed the non-toxic nature of CMC–Nb and Alg–mTz towards HEK-293 cells. Furthermore, the ICG-loaded hydrogels effectively inhibited the metabolic activity of Hela cells after NIR exposure.
Optical Genome Mapping Helps to Identify BCR::JAK2 Rearrangement Arising from Cryptic Complex Chromosomal Aberrations: A Case Report and Literature Review
Authors: Vanjari Neelam , Tang Guilin , Toruner Gokce A. , Wang Wei , Thakral Beenu , Zhao Ming , Dave Bhavana J. , Khoury Joseph D. , Medeiros L. Jeffrey , Tang Zhenya
Published: December 2023

We report a case of myeloproliferative neoplasm, not otherwise specified (MPN-NOS)-transformed AML with BCR::JAK2 rearrangement. Chromosomal analysis indicated a simple abnormal karyotype 46,XY,t(7;17)(q21;q24),t(9;22)(p24;q11.2). Fluorescence in situ hybridization (FISH) using a BCR/ABL1/ASS1 probe set suggested a possible BCR rearrangement and a reflex JAK2 breakapart probe indicated JAK2 rearrangement, most likely partnered with BCR. Optical genome mapping (OGM) analysis confirmed BCR::JAK2 derived through an inv(9)(p24p13) after a t(9;22)(p13;q11.2) in this case. Due to the complexity of chromosomal aberrations, disruption and/or rearrangement of other genes such as KIF24::BCR, JAK2::KIF24/UBAP1, and CDK6:SOX9 were also identified by OGM. Although the functionality and clinical importance of these novel rearrangements were unknown, disruption of these genes might be associated with a poorer response to chemotherapy and disease progression. We also reviewed all cases with BCR::JAK2 rearrangement reported in the literature. In conclusion, a suspected t(9;22)/BCR::JAK2 rearrangement warrants further characterization with genomic assays such as OGM, whole chromosome sequencing, and RNA sequencing to explore other gene disruptions and/or rearrangements.
Exploring Resilience in Care Home Nurses: An Online Survey
Authors: Mallon Anita , Mitchell Gary , Carter Gillian , Francis McLaughlin Derek , Linden Mark , Brown Wilson Christine
Published: December 2023

Resilience is considered a core capability for nurses in managing workplace challenges and adversity. The COVID-19 pandemic has brought care homes into the public consciousness; yet, little is known about the resilience of care home nurses and the attributes required to positively adapt in a job where pressure lies with individuals to affect whole systems. To address this gap, an online survey was undertaken to explore the levels of resilience and potential influencing factors in a sample of care home nurses in Northern Ireland between January and April 2022. The survey included the Connor–Davidson Resilience Scale, demographic questions and items relating to nursing practice and care home characteristics. Mean differences and key predictors of higher resilience were explored through statistical analysis. A moderate level of resilience was reported among the participants (n = 56). The key predictors of increased resilience were older age and higher levels of education. The pandemic has exposed systemic weakness but also the strengths and untapped potential of the care home sector. By linking the individual, family, community and organisation, care home nurses may have developed unique attributes, which could be explored and nurtured. With tailored support, which capitalises on assets, they can influence a much needed culture change, which ensures the contribution of this sector to society is recognised and valued.
Use of the Therapy App Prescinde for Increasing Adherence to Smoking Cessation Treatment
Authors: López-Torrecillas Francisca , Ramírez-Uclés Isabel , Rueda María del Mar , Cobo-Rodríguez Beatriz , Castro-Martín Luis , Urrea-Castaño Sabina Arantxa , Muñoz-López Lucas
Published: December 2023

Tobacco use poses major health risks and is a major contributor to causes of death worldwide. Mobile phone-based cessation apps for this substance are gaining popularity, often used as a component of traditional interventions. This study aimed to analyze adherence to an intervention using a mobile phone application (App-therapy Prescinde (v1)) as a function of sociodemographic variables (age, gender, educational level, and profession) as well as the primary activities supported by the app (reducing tobacco or cannabis use and increasing physical exercise). The participants were recruited through the web pages of the Occupational Risk Prevention Service and the Psychology Clinic of the University of Granada during the COVID-19 confinement period. The application’s contents include three components (self-report, motivational phrases, and goal setting). Our findings indicate that being male, being aged between 26 and 62, having a high school education, and being unemployed increase the likelihood of adherence to the Prescinde therapy app three months after usage. Our findings highlight the importance of developing new therapeutic approaches and conducting in-depth studies on the factors associated with adherence to tobacco cessation and cannabis cessation treatments via mobile phone applications.
Effects of Communication Skills and Organisational Communication Satisfaction on Self-Efficacy for Handoffs among Nurses in South Korea
Authors: Lee Yongmi , Kim Hyekyoung , Oh Younjae
Published: December 2023

(1) Background: Although numerous studies related to communication in a nursing context have been conducted, there is a lack of research considering the effects of personal and organisational communication factors on the self-efficacy for handoffs. This study aimed to identify the impact of communication competence and intra-organisational communication satisfaction on self-efficacy for handoffs among nurses. (2) Methods: This cross-sectional research was conducted between September and October 2018. In total, 203 registered nurses were invited to participate in the study by convenience sampling from five general hospitals in South Korea. Data were analysed using SPSS for t-test, one-way analysis of variance, Pearson’s correlation coefficients, and multiple regression analysis. (3) Results: In the final regression model, the adjusted R square was significant, explaining 24.2% of the variance in self-efficacy for handoffs (F = 22.43, p = 0.001) when the variable horizontal communication (β = 0.282, p < 0.001) was included in intra-organisational communication satisfaction. In addition, the longer the nurse’s experience in the current unit and the higher the communication competence, the more statistically significant the self-efficacy for handoffs was found to be (β = 0.215, p = 0.001 and β = 0.180, p = 0.008). (4) Conclusions: To enhance the self-efficacy for handoffs, nurse managers should foster an atmosphere that allows their staff nurses to interact freely and establish specific guidelines for handoffs through mutual communication.
Blastic Plasmocytoid Dendritic Cell Neoplasm (BPDCN): Clinical Features and Histopathology with a Therapeutic Overview
Authors: Cazzato Gerardo , Capuzzolo Marialessandra , Bellitti Emilio , De Biasi Giovanni , Colagrande Anna , Mangialardi Katia , Gaudio Francesco , Ingravallo Giuseppe
Published: December 2023

Blastic Plasmacytoid Dendritic Cell Neoplasms (BPDCNs) are a rare, highly aggressive hematological malignant neoplasm that primarily involve the skin, bone marrow, lymph nodes and even extra-nodal sites. The rarity and relative poor description of cases in the literature make it necessary to review and further studies that deeply investigate this entity not only in a histopathological but also molecular field. In August–September 2023, we searched MEDLINE, PubMed and Scopus for randomized controlled trials (RCTs), narrative and systematic reviews, meta-analyses, observational studies (either longitudinal or retrospective), and case series published in English in the last 25 years using the keywords BPDCN, PDCs, Blastic NK-cell lymphoma, agranular CD4+ NK leukemia/lymphoma, agranular CD4+ CD56+ hematodermic neoplasm/tumor. Despite the progress made in recent years in the diagnosis and biological understanding of the disease, until 2018 there was no clear consensus regarding its treatment and the main therapeutic schemes used were based on chemotherapy regimens already used in the treatment of lymphomas, acute lymphoblastic leukemia (ALL) and/or acute myeloid leukemia (AML). In this narrative review, we address the definition and epidemiological features of BPDCN, provide the different theories on the etiopathogenesis with particular attention to the presumed cell of origin, discuss the main clinical manifestations that provide a sign of its presence, summarize the main histopathological and immunophenotypic characteristics with special attention to the most important markers, and finally, we provide some of the most effective information on the therapeutic treatment modalities of BPDCN.
Video Game Playing and Internet Gaming Disorder: A Profile of Young Adolescents
Authors: Labrador Marta , Sánchez-Iglesias Iván , Bernaldo-de-Quirós Mónica , Estupiñá Francisco J. , Fernandez-Arias Ignacio , Vallejo-Achón Marina , Labrador Francisco J.
Published: December 2023

In recent times, growing concern has arisen regarding the utilization of technology, video games, and the emergence of internet gaming disorder (IGD), particularly among young adolescents. This worry arises from the ambiguity in distinguishing between “normal” and “problematic” video game behavior, despite efforts to establish clear criteria for defining both. The goal of this study is to outline distinct profiles of adolescent video game players and identify variables associated with their gaming practices that correlate with problematic gaming. The study utilizes a substantial sample of adolescents drawn from a representative cross-section of educational institutions in the city of Madrid, ranging in age from 12 to 16 years. In total, 1516 participants (75%) acknowledged engaging in video game activities. The research delves into characterizing prevailing profiles of video game participants within this cohort and scrutinizes the profile that aligns with issues of IGD. In summary, approximately three-quarters of young adolescents participate in video gaming, with males constituting the majority. Typically, participants immerse themselves in action genre games for over three days per week, with males exhibiting a higher frequency than their female counterparts. Elevated gaming frequency correlates with heightened IGD scores, particularly among females. Young adolescents show a preference for game consoles (males) and mobile phones (females) and often play alone at home. Specific factors such as the device used, online mode, company, and gaming location impact the IGD scores. These profiles aim to assist families and educators in recognizing potential risk behaviors and IGD concerns; however, it is crucial to emphasize the necessity for case-specific screening and evaluation before deliberating on such behaviors.
“Health Outcomes of Grandparents Caring for Double Orphans in South Africa”: What Are the Determinants?
Authors: Likoko Salmon , Akokuwebe Monica Ewomazino , Osuafor Godswill Nwabuisi , Idemudia Erhabor Sunday
Published: December 2023

In the 21st century, grandparenthood is a significant phenomenon in the fields of demography, gerontology, and sociology. It is mainly explored in the context of ageing, as it is poised to become one of the most significant demographic phenomena and social issues in contemporary South Africa. Therefore, this study examined the determinants associated with grandparents who are parenting as caregivers and the health challenges they are exposed to as caregivers. The National Income Dynamics Study (NIDS) Wave 5 dataset was utilised, and a total of 302,476 grandparents aged 25 years and older, who were reported to be primary caregivers of double orphans, were included in the analysis. Both bivariate and multivariate binary logistic regressions were performed to determine the predictors of the determinants of grandparents parenting as caregivers and their health challenges in South Africa. Estimated odds ratios (ORs) with 95% confidence intervals (CIs) were used, and the threshold for statistical significance was established at ρ < 0.05. A majority of the male and female grandparent caregivers were aged 24–34 years, were Black Africans (69.8%), had secondary education (46.9%), reported health challenges (HC) (59.7%), with 26.4% reporting headaches in the last 30 days. Logistic regression revealed that grandparent caregivers aged 55–64 years were 8.9 times more likely to report health challenges compared to those aged 25–34 years. Non-Black African grandparent caregivers were found to be 0.61 times less likely to report health challenges, compared to Black African grandparent caregivers. Those with perceived poor health status were 3.3 times more likely to report health challenges, compared to those with excellent perceived health status. Therefore, there is an urgent need to redesign health interventions to address these health burdens among grandparent caregivers and to take cognisance of providing economic and social support for these vulnerable populations.
Impact of rs1805127 and rs55742440 Variants on Atrial Remodeling in Hypertrophic Cardiomyopathy Patients with Atrial Fibrillation: A Romanian Cohort Study
Authors: Popa-Fotea Nicoleta-Monica , Oprescu Nicoleta , Scafa-Udriste Alexandru , Micheu Miruna Mihaela
Published: December 2023

Atrial fibrillation (AFib) is characterized by a complex genetic component. We aimed to investigate the association between variations in genes related to cardiac ion handling and AFib in a cohort of Romanian patients with hypertrophic cardiomyopathy (HCM). Forty-five unrelated probands with HCM were genotyped by targeted next-generation sequencing (NGS) for 24 genes associated with cardiac ion homeostasis. Subsequently, the study cohort was divided into two groups based on the presence (AFib+) or absence (AFiB−) of AFib detected during ECG monitoring. We identified two polymorphisms (rs1805127 located in KCNE1 and rs55742440 located in SCN1B) linked to AFib susceptibility. In AFib+, rs1805127 was associated with increased indexed left atrial (LA) maximal volume (LAVmax) (58.42 ± 21 mL/m2 vs. 32.54 ± 6.47 mL/m2, p < 0.001) and impaired LA strain reservoir (LASr) (13.3 ± 7.5% vs. 24.4 ± 6.8%, p < 0.05) compared to those without respective variants. The rs55742440 allele was less frequent in patients with AFib+ (12 out of 25, 48%) compared to those without arrhythmia (15 out of 20, 75%, p = 0.05). Also, AFib+ rs55742440 carriers had significantly lower LAVmax compared to those who were genotype negative. Among patients with HCM and AFib+, the rs1805127 variant was accompanied by pronounced LA remodeling, whereas rs55742440’s presence was related to a milder LA enlargement.
Opportunities in Cancer Therapies: Deciphering the Role of Cancer Stem Cells in Tumour Repopulation
Authors: Marcu Loredana G. , Dell’Oro Mikaela , Bezak Eva
Published: December 2023

Tumour repopulation during treatment is a well acknowledged yet still challenging aspect of cancer management. The latest research results show clear evidence towards the existence of cancer stem cells (CSCs) that are responsible for tumour repopulation, dissemination, and distant metastases in most solid cancers. Cancer stem cell quiescence and the loss of asymmetrical division are two powerful mechanisms behind repopulation. Another important aspect in the context of cancer stem cells is cell plasticity, which was shown to be triggered during fractionated radiotherapy, leading to cell dedifferentiation and thus reactivation of stem-like properties. Repopulation during treatment is not limited to radiotherapy, as there is clinical proof for repopulation mechanisms to be activated through other conventional treatment techniques, such as chemotherapy. The dynamic nature of stem-like cancer cells often elicits resistance to treatment by escaping drug-induced cell death. The aims of this scoping review are (1) to describe the main mechanisms used by cancer stem cells to initiate tumour repopulation during therapy; (2) to present clinical evidence for tumour repopulation during radio- and chemotherapy; (3) to illustrate current trends in the identification of CSCs using specific imaging techniques; and (4) to highlight novel technologies that show potential in the eradication of CSCs.
How Do Phages Disrupt the Structure of Enterococcus faecalis Biofilm?
Authors: Moryl Magdalena , Różalski Antoni , de Figueiredo Jose Antonio Poli , Palatyńska-Ulatowska Aleksandra
Published: December 2023

Biofilms are composed of multicellular communities of microbial cells and their self-secreted extracellular polymeric substances (EPS). The viruses named bacteriophages can infect and lyze bacterial cells, leading to efficient biofilm eradication. The aim of this study was to analyze how bacteriophages disrupt the biofilm structure by killing bacterial cells and/or by damaging extracellular polysaccharides, proteins, and DNA. The use of colorimetric and spectrofluorimetric methods and confocal laser scanning microscopy (CLSM) enabled a comprehensive assessment of phage activity against E. faecalis biofilms. The impact of the phages vB_Efa29212_2e and vB_Efa29212_3e was investigated. They were applied separately or in combination on 1-day and 7-day-old biofilms. Phages 2e effectively inhibited the growth of planktonic cells with a limited effect on the biofilm. They did not notably affect extracellular polysaccharides and proteins; however, they increased DNA levels. Phages 3e demonstrated a potent and dispersing impact on E. faecalis biofilms, despite being slightly less effective than bacteriophages 2e against planktonic cells. Phages 3e reduced the amount of extracellular polysaccharides and increased eDNA levels in both 1-day-old and 7-day-old biofilm cultures. Phage cocktails had a strong antimicrobial effect on both planktonic and biofilm-associated bacteria. A significant reduction in the levels of polysaccharides, proteins, and eDNA in 1-day-old biofilm samples was noted, which confirms that phages interfere with the structure of E. faecalis biofilm by killing bacterial cells and affecting extracellular polymer levels.
The Effects of Larval Cryopreservation on the Epigenetics of the Pacific Oyster Crassostrea gigas
Authors: Liu Yibing , Bao Lisui , Catalano Sarah R. , Zhu Xiaochen , Li Xiaoxu
Published: December 2023

High mortalities and highly variable results during the subsequent development of post-thaw larvae have been widely considered as key issues restricting the application of cryopreservation techniques to support genetic improvement programs and hatchery production in farmed marine bivalve species. To date, few studies have been undertaken to investigate the effects of cryodamage at the molecular level in bivalves. This study is the first to evaluate the effect of larval cryopreservation on the epigenetics of the resultant progenies of the Pacific oyster Crassostrea gigas. The results show that the level of DNA methylation was significantly (p < 0.05) higher and lower than that of the control when the trochophore larvae were revived and when they developed to D-stage larvae (day 1 post-fertilization), respectively, but the level returned to the control level from day 8 post-fertilization onwards. The expression of the epigenetic regulator genes DNMT3b, MeCP2, JmjCA, KDM2 and OSA changed significantly (p < 0.05) when the trochophore larvae were thawed, and then they reverted to the control levels at the D- and later larval developmental stages. However, the expression of other epigenetic regulator genes, namely, MBD2, DNMT1, CXXC1 and JmjD6, did not change at any post-thaw larval developmental stage. For the newly thawed trochophore larvae, the amount of methylated H3K4Me1 and H3K27Me1 significantly changed, and the expression of all Jumonji orthologs, except that of Jumonji5, significantly (p < 0.05) decreased. These epigenetic results agree with the data collected on larval performances (e.g., survival rate), suggesting that the effect period of the published cryopreservation technique on post-thaw larvae is short in C. gigas.
The Influence of the Nature of the Polymer Incorporating the Same A3B Multifunctional Porphyrin on the Optical or Electrical Capacity to Recognize Procaine
Authors: Lascu Anca , Vlascici Dana , Birdeanu Mihaela , Epuran Camelia , Fratilescu Ion , Fagadar-Cosma Eugenia
Published: December 2023

The multifunctionality of an A3B mixed-substituted porphyrin, namely 5-(4-carboxyphenyl)-10,15,20-tris(4-methylphenyl)porphyrin (5-COOH-3MPP), was proven due to its capacity to detect procaine by different methods, depending on the polymer matrix in which it is incorporated. The hybrid nanomaterial containing k-carrageenan and AuNPs (5-COOH-3MPP-k-carrageenan-AuNPs) was able to optically detect procaine in the concentration range from 5.76 × 10−6 M to 2.75 × 10−7 M, with a limit of detection (LOD) of 1.33 × 10−7 M. This method for the detection of procaine gave complementary results to the potentiometric one, which uses 5-COOH-3MPP as an electroactive material incorporated in a polyvinylchloride (PVC) membrane plasticized with o-NPOE. The detected concentration range by this ion-selective membrane electrode is wider (enlarged in the field of higher concentrations from 10−2 to 10−6 M), linearly dependent with a 53.88 mV/decade slope, possesses a detection limit of 7 × 10−7 M, a response time of 60 s, and has a certified stability for a working period of six weeks.
Amphiphilic Sialic Acid Derivatives as Potential Dual-Specific Inhibitors of Influenza Hemagglutinin and Neuraminidase
Authors: Lőrincz Eszter Boglárka , Herczeg Mihály , Houser Josef , Rievajová Martina , Kuki Ákos , Malinovská Lenka , Naesens Lieve , Wimmerová Michaela , Borbás Anikó , Herczegh Pál , Bereczki Ilona
Published: December 2023

In the shadow of SARS-CoV-2, influenza seems to be an innocent virus, although new zoonotic influenza viruses evolved by mutations may lead to severe pandemics. According to WHO, there is an urgent need for better antiviral drugs. Blocking viral hemagglutinin with multivalent N-acetylneuraminic acid derivatives is a promising approach to prevent influenza infection. Moreover, dual inhibition of both hemagglutinin and neuraminidase may result in a more powerful effect. Since both viral glycoproteins can bind to neuraminic acid, we have prepared three series of amphiphilic self-assembling 2-thio-neuraminic acid derivatives constituting aggregates in aqueous medium to take advantage of their multivalent effect. One of the series was prepared by the azide-alkyne click reaction, and the other two by the thio-click reaction to yield neuraminic acid derivatives containing lipophilic tails of different sizes and an enzymatically stable thioglycosidic bond. Two of the three bis-octyl derivatives produced proved to be active against influenza viruses, while all three octyl derivatives bound to hemagglutinin and neuraminidase from H1N1 and H3N2 influenza types.
Fitness of Nutrition Regulation in a Caterpillar Pest Mythimna separata (Walker): Insights from the Geometric Framework
Authors: Sun Shaolei , Yang Zhen , Ren Jinchan , Liu Tongxian , Jing Xiangfeng
Published: December 2023

In nature, plants can contain variable nutrients depending upon the species, tissue, and developmental stage. Insect herbivores may regulate their nutrient intake behaviorally and physio- logically when encountering different foods. This study examined the nutritional regulation of the oriental armyworm, Mythimna separata, for the first time. In one experiment, we allowed the cater-pillars to choose between two nutritionally balanced but complementary diets. The caterpillars did not randomly consume the paired foods, but instead chose between the nutritionally balanced but complementary diets. This intake behavior was found to change with their developmental stages. Furthermore, the nutrient concentrations in food significantly impacted the insect’s performance. In the other experiment, caterpillars were given one of eleven diets that reflected the different nutrient conditions in the field. The results showed that proteins were significantly associated with developmental time and fecundity. For example, by consuming protein-biased food, the caterpillars developed faster and produced more eggs. In contrast, carbohydrates were more strongly linked to lipid accumulation, and caterpillars accumulated more lipids when consuming the carbohydrate-biased food. Moreover, the caterpillars were also found to actively regulate their intake of proteins and carbohydrates based on food quality and to physiologically prepare for subsequent life stages. These findings enhance our understanding of how M. separata feeds and responds to different nutritional environments in the field, which could have implications for managing insect herbivores in agricultural settings.
The Related Factors and Effect of Electrode Displacement on Motor Outcome of Subthalamic Nuclei Deep Brain Stimulation in Parkinson’s Disease
Authors: Yuan Tianshuo , Chen Yingchuan , Zhu Guanyu , Zhang Jianguo
Published: December 2023

Background: Previous studies have revealed the existence of electrode displacement during subthalamic nucleus deep brain stimulation (STN-DBS). However, the effect of electrode displacement on treatment outcomes is still unclear. In this study, we aimed to analyze the related factors of electrode displacement and assess postoperative electrode displacement in relation to the motor outcomes of STN-DBS. Methods: A total of 88 patients aged 62.73 ± 6.35 years (55 males and 33 females) with Parkinson’s disease undergoing STN-DBS, with comprehensive clinical characterization before and 1 month after surgery, were involved retrospectively and divided into a cross-incision group and cannula puncture group according to different dura opening methods. The electrode displacement, unilateral pneumocephalus volume percent (uPVP), and brain volume percent were estimated. Results: A significant anterior and lateral electrode displacement was observed among all implanted electrodes after pneumocephalus absorption (p < 0.0001). The degree of electrode displacement was positively correlated with the uPVP (p = 0.005) and smaller in females than males (p = 0.0384). Electrode displacement was negatively correlated with motor improvement following STN-DBS in both on-medication and off-medication conditions (p < 0.05). Dural puncture reduced the uPVP (p < 0.0001) and postoperative electrode displacement (p = 0.0086) compared with dural incision. Conclusions: Electrode displacement had a negative impact on the therapeutic efficacy of STN-DBS. Opening the dura via cannula puncture is recommended to increase the accuracy of the lead implantation.