bluuebunny/crossref_metadata_2025 huggingface dataset:
The Referee as an Educator: Assessment of the Quality of Referee–Players Interactions in Competitive Youth Handball
Authors: Płoszaj Katarzyna , Firek Wiesław , Czechowski Marcin
Published: June 2020

Sport does not automatically generate educational benefits for players. For a sports field to become a child-friendly educational environment, it is essential that all actors involved in the organization of youth sport take deliberate educational measures. Among these actors are referees, who should be taken into account during the research on the educational value of sport for the youngest. The subject of the present study was handball referees, who interact with the players during matches. Assuming that the referee is an important actor in sport education and that referee–players interactions are the basic mechanism of the referee’s educational influence, this study aimed to assess the quality of his or her interactions with players during handball matches for children aged 9 to 12 years. The research was conducted in a group of 25 handball referees who refereed matches of children in the region of Mazowieckie Voivodeship in Poland. The referees surveyed had current licenses issued by the Warsaw–Mazovian Handball Association. To assess the quality of referee–players interactions, the authors’ direct observation tool (Referee–Players’ Interaction Assessment Scoring System) was used. The educational referee–players interaction was studied in six dimensions: Positive climate, Responsiveness, Behavior management, Proficiency, Instructing, and Communicating. Data were statistically analyzed using chi-squared test, Mann–Whitney U test and exploratory factor analysis (EFA). Cronbach’s alpha values were higher than 0.90 in the factors, showing adequate levels of reliability. The results of the research demonstrated that the assessment of the quality of the referee’s educational influence on players was neither affected by the referees’ experience nor by the outcome of the match. The quality of educational referee–players interactions in five of the six dimensions studied was assessed as average, whereas positive climate was assessed as poor (three-step scale: poor, average, good). If referees are to support coaches and parents in achieving their educational goals, the results indicate areas where they can improve. The research provided empirical evidence that could be used as a basis for the modification of previous training programs for referees developed by local and national sports associations. The referees should be trained to build a positive climate on the sport field, which consists in creating emotional ties with players (physical proximity, social conversation), expressed in an enthusiastic attitude and joy of contacts (smiling, engagement, positive affect reaction, positive comments, respectful and inclusive language, using players first names, listening to players). In addition, referees must be taught to actively monitor players’ emotional, cognitive, social, and health needs, as well as to respond to the players’ needs and solve problems.
Prevalence, Disability and Associated Factors of Playing-Related Musculoskeletal Pain among Musicians: A Population-Based Cross-Sectional Descriptive Study
Authors: Gómez-Rodríguez Rosa , Díaz-Pulido Belén , Gutiérrez-Ortega Carlos , Sánchez-Sánchez Beatriz , Torres-Lacomba María
Published: June 2020

Background: Playing-related musculoskeletal disorders are the most frequent complaints among instrumental musicians. The aims of this study were: to assess the prevalence of musculoskeletal pain; to evaluate neck, shoulder, and lower back disability; and to determine the associated factors with the presence of musculoskeletal pain among musicians. Methods: A population-based, cross-sectional descriptive study was conducted. We selected Spaniard musicians over 16 years old who played a musical instrument for at least five hours per week. They answered the Spanish versions of the Standardised Nordic Questionnaire, the Oswestry Disability Index, Neck Disability Index and Shoulder Pain and Disability Index. Results: We found 94.8% of musicians presented at least one symptomatic region in the last 12 months, and 72.3% in the last seven days. Female musicians (OR 4.38, CI 2.11−9.12), musicians with overweight or obesity (OR 5.32, CI 2.18−12.97), and musicians who play more than 14 h per week (OR 3.86, CI 1.80−8.29)were shown to be a higher risk of suffering musculoskeletal pain. Conclusions: Musculoskeletal disorders symptoms are highly prevalent in musicians. The main risk factors related to musculoskeletal disorders symptoms were gender (being female), overweight, obesity, and spending playing more than 14 h a week practicing. This study highlights the need to provide strategies to prevent occupational disabilities among musicians. Further studies are needed to analyse the prevalence of pain in the musician using other sampling methods.
The Nexus between Workplace Exposure for Wood, Welding, Motor Mechanic, and Oil Refinery Workers and the Prevalence of Prediabetes and Type 2 Diabetes Mellitus
Authors: Meo Sultan Ayoub , Al-khlaiwi Thamir , Abukhalaf Abdulelah Adnan , Alomar Ali Abdullah , Alessa Omar Mohammed , Almutairi Faris Jamal , Alasbali Majed Mohammed
Published: June 2020

Workplace exposure in various occupational and industrial sectors is an emerging health concern worldwide. This study aimed to investigate the nexus between workplace exposure for wood, welding, motor mechanic, and oil refinery workers and the prevalence of prediabetes and type 2 diabetes mellitus. Initially, 2500 male volunteers who were wood, welding, motor mechanic, and oil refinery workers were interviewed. After an examination of their demographics and medical history, 1408 non-smoking wood (158), welding (560), motor mechanic (272), and oil refinery workers (217), along with 201 control subjects, were selected. The participants’ mean age was 36.59 ± 0.29 years and the mean body mass index was 26.14 ± 0.11 kg/m2. The selected industry workers had been exposed to their respective wood, welding, motor mechanic, and oil refinery workplaces for 8 h per day, six days per week. The American Diabetic Association (ADA)-based glycated hemoglobin (HbA1c) criterion was used to diagnose prediabetes and type 2 diabetes mellitus. Subjects with an HbA1c of less than 5.7% were regarded as non-diabetics, subjects with an HbA1c of 5.7%–6.4% were considered prediabetics, and subjects with an HbA1c of more than 6.4% were considered diabetics. In wood industry workers, the prevalence of prediabetes (PD) was 64 (40.50%) and in type 2 diabetes mellitus (T2DM), it was 21 (13.29%); in welding workers, the prevalence of prediabetes was 261 (46.60%), and for T2DM, it was 90 (16.07%); in motor mechanic workers, the prevalence of prediabetes was 110 (40.44%), and for T2DM, it was 126 (46.32%); and in oil refinery workers, the prevalence of prediabetes was 80 (36.86%), and for T2DM, it was 35 (16.12%). However; the combined prevalence of prediabetes and T2DM among wood, welding, motor mechanic, and oil refinery workers was 421 (34.79%) and 515 (42.66%), respectively. The prevalence of prediabetes and T2DM among workers increased with the duration of working exposure in the wood, welding, motor mechanic, and oil refinery industries. A one-year working exposure in these industries caused an increase of 0.03% in HbA1c. Workplace exposure in wood, welding, motor mechanic, and oil refinery industries increased the risk of prevalence of prediabetes and T2DM among the workers and affected the diabetes etiology.
Responsible Job Design Based on the Internal Social Responsibility of Local Governments
Authors: Sánchez-Hernández M. Isabel , Stankevičiūtė Živilė , Robina-Ramirez Rafael , Díaz-Caro Carlos
Published: June 2020

Sustainability needs the socially responsible orientation of public institutions, but not only externally. How civil servants and municipal employees consider what local governments do in relation to their internal social responsibility is the main question of this study. Through structural equation modelling and a sample of 294 employees in local governments in a Spanish region (Extremadura), it is demonstrated that responsible job position design, as well as good communication and team building, both have positive and significant effects on the personal identification of employees with the city council. That has been interpreted as the essence of internal social responsibility and the first step for building external social responsibility at the municipal level. Practical implications and future directions are discussed.
The Development and Effectiveness of a Clinical Training Violence Prevention Program for Nursing Students
Authors: Jeong Yunhwa , Lee Kyunghee
Published: June 2020

The study aimed to develop and evaluate a violence prevention program for nursing students to improve communication self-efficacy, problem-focused coping style, emotion-focused coping style, and the ability to cope with violence. Using an eight-session violence prevention program, the study was designed as quasi experimental, with a pretest, posttest, and follow-up assessment with a nonequivalent control group. Nursing students from the fourth year of a university were selected as participants; 22 students were assigned to the experimental group and 23 to the control group. Data analysis included Chi-square, Fisher’s exact test, Levene’s Test, Mann–Whitney U-test, and repeated measures ANOVA. Results showed that the experimental group showed significantly higher posttest scores for the problem-focused coping style (F = 20.77, p < 0.001), intra-individual and interaction effects for the emotion-focused coping style (F = 12.03, p < 0.001), and the ability to cope with violence (U = 70, p < 0.001) than the control group. Thus, the workplace violence prevention program was effective for nursing students.
Chronic Diseases and Associated Factors among Older Adults in Loja, Ecuador
Authors: Bonilla-Sierra Patricia , Vargas-Martínez Ana-Magdalena , Davalos-Batallas Viviana , Leon-Larios Fatima , Lomas-Campos Maria-de-las-Mercedes
Published: June 2020

(1) Background: This study aimed to explore the symptoms, functional status, and depression in patients with chronic diseases in Loja, Ecuador. (2) Methods: A cross-sectional study was carried out with patients over 60 years old having at least one chronic disease and cared for in healthcare centers of the Health Ministry of Ecuador or living in associated geriatric centers. (3) Results: The sample comprised 283 patients with a mean age of 76.56 (SD 7.76) years. The most prevalent chronic diseases were chronic obstructive pulmonary disease, followed by arterial hypertension and diabetes. Patients with a joint disease had the worst scores for the majority of the symptoms assessed with the Edmonton Scale. Cancer, dementia, and arterial hypertension contributed the most to the dependence levels assessed with the Barthel Index. Dementia contributed the most to the poor performance status evaluated with the Karnofsky Performance Status. Cancer and diabetes contributed the most to depression. Patients with a higher number of chronic diseases reported worse functional status. (4) Conclusions: Targeted interventions to address symptoms, functional status, and depression in patients with chronic diseases are needed.
Can Fitness Education Programs Satisfy Fitness Professionals’ Competencies? Integrating Traditional and Revised Importance-Performance Analysis and Three-Factor Theory
Authors: Ku Gordon Chih Ming , Hsieh Chi-Ming
Published: June 2020

The purpose of this study is to assess whether fitness education programs could meet the needs or competencies of fitness professionals such as personal trainers and group fitness instructors. A mixed method was adopted to address the objectives of the study. In the first step, a semi-structured interview was conducted with five fitness experts to identify the five dimensions of professional competencies. In the second step, an online survey and paper questionnaires were utilized to collect data from 324 eligible subjects. Traditional importance-performance analysis, revised importance-performance analysis, and the three-factor theory were used to analyze the collected data. The results indicate that “professional skill,” “career development,” and “public relations” are the three most critical professional competencies. “Nutrition” and “coping with stress” should be strengthened and improved in fitness education programs. “Administrative management” is the least important professional competency. Multi-competencies development and lifelong learning are the factors for a successful fitness trainer.
Changes in Gambling Behavior during the COVID-19 Pandemic—A Web Survey Study in Sweden
Authors: Håkansson Anders
Published: June 2020

The COVID-19 pandemic has dramatically changed everyday life, and policy makers have raised concerns about possible changes in gambling patterns during the pandemic. This study aimed to examine whether self-reported gambling has increased during the pandemic, and to examine potential correlates of such a change. This general population survey study in Sweden collected self-report data from 2016 web survey members (51 percent men, nine percent moderate-risk/problem gamblers). Correlates of increased gambling and increased gambling specifically due to COVID-19-related cancellation of sports were calculated. Four percent reported an overall gambling increase during the pandemic. The proportion of individuals reporting an increase, compared to individuals reporting a decrease, was markedly higher for online casinos (0.62), online horse betting (0.76) and online lotteries (0.73), and lower for sports betting (0.11). Overall, gambling increases were independently associated with gambling problems and increased alcohol consumption. In the sub-group, where there was an increase in specific gambling types in response to cancelled sports betting events, rates of gambling problems were high. In conclusion, only a minority report increased gambling in response to the pandemic, but this group has markedly higher gambling problems and changes in alcohol consumption, and may represent a sub-group with a particularly high vulnerability. This calls for preventive action in people with higher gambling risks in response to the pandemic.
Predicting Student Well-Being: Network Analysis Based on PISA 2018
Authors: Govorova Elena , Benítez Isabel , Muñiz José
Published: June 2020

The latest trends in research extend the focus of school effectiveness beyond students’ acquisition of knowledge and skills, looking at aspects such as well-being in the academic context. Although the concept of well-being itself has been defined and measured in various ways, neither its dimensions nor the relationships between the components have been clearly described. The aim of the present study was to analyse how the elements of well-being interact and determine how they are influenced by school factors. To do that, we conducted a network analysis based on data from the Programme for International Student Assessment (PISA) 2018 international assessment. Our results demonstrated that cognitive, psychological, and social well-being variables form a solid welfare construct in the educational context, where students’ resilience and fear of failure, along with their sense of belonging, play central roles. Although the influence of school factors on student well-being is generally low, teaching enthusiasm and support promote positive school climates which are, in turn, crucial in reducing bullying.
Ionising Radiation Induces Promoter DNA Hypomethylation and Perturbs Transcriptional Activity of Genes Involved in Morphogenesis during Gastrulation in Zebrafish
Authors: Murat El Houdigui Sophia , Adam-Guillermin Christelle , Armant Olivier
Published: June 2020

Embryonic development is particularly vulnerable to stress and DNA damage, as mutations can accumulate through cell proliferation in a wide number of cells and organs. However, the biological effects of chronic exposure to ionising radiation (IR) at low and moderate dose rates (< 6 mGy/h) remain largely controversial, raising concerns for environmental protection. The present study focuses on the molecular effects of IR (0.005 to 50 mGy/h) on zebrafish embryos at the gastrula stage (6 hpf), at both the transcriptomics and epigenetics levels. Our results show that exposure to IR modifies the expression of genes involved in mitochondrial activity from 0.5 to 50 mGy/h. In addition, important developmental pathways, namely, the Notch, retinoic acid, BMP and Wnt signalling pathways, were altered at 5 and 50 mGy/h. Transcriptional changes of genes involved in the morphogenesis of the ectoderm and mesoderm were detected at all dose rates, but were prominent from 0.5 to 50 mGy/h. At the epigenetic level, exposure to IR induced a hypomethylation of DNA in the promoter of genes that colocalised with both H3K27me3 and H3Kme4 histone marks and correlated with changes in transcriptional activity. Finally, pathway enrichment analysis demonstrated that the DNA methylation changes occurred in the promoter of important developmental genes, including morphogenesis of the ectoderm and mesoderm. Together, these results show that the transcriptional program regulating morphogenesis in gastrulating embryos was modified at dose rates greater than or equal to 0.5 mGy/h, which might predict potential neurogenesis and somitogenesis defects observed at similar dose rates later in development.
Concordance between Thioacetamide-Induced Liver Injury in Rat and Human In Vitro Gene Expression Data
Authors: Schyman Patric , Printz Richard L. , Estes Shanea K. , O’Brien Tracy P. , Shiota Masakazu , Wallqvist Anders
Published: June 2020

The immense resources required and the ethical concerns for animal-based toxicological studies have driven the development of in vitro and in silico approaches. Recently, we validated our approach in which the expression of a set of genes is uniquely associated with an organ-injury phenotype (injury module), by using thioacetamide, a known liver toxicant. Here, we sought to explore whether RNA-seq data obtained from human cells (in vitro) treated with thioacetamide-S-oxide (a toxic intermediate metabolite) would correlate across species with the injury responses found in rat cells (in vitro) after exposure to this metabolite as well as in rats exposed to thioacetamide (in vivo). We treated two human cell types with thioacetamide-S-oxide (primary hepatocytes with 0 (vehicle), 0.125 (low dose), or 0.25 (high dose) mM, and renal tubular epithelial cells with 0 (vehicle), 0.25 (low dose), or 1.00 (high dose) mM) and collected RNA-seq data 9 or 24 h after treatment. We found that the liver-injury modules significantly altered in human hepatocytes 24 h after high-dose treatment involved cellular infiltration and bile duct proliferation, which are linked to fibrosis. For high-dose treatments, our modular approach predicted the rat in vivo and in vitro results from human in vitro RNA-seq data with Pearson correlation coefficients of 0.60 and 0.63, respectively, which was not observed for individual genes or KEGG pathways.
Bacterial Actin-Specific Endoproteases Grimelysin and Protealysin as Virulence Factors Contributing to the Invasive Activities of Serratia
Authors: Khaitlina Sofia , Bozhokina Ekaterina , Tsaplina Olga , Efremova Tatiana
Published: June 2020

The article reviews the discovery, properties and functional activities of new bacterial enzymes, proteases grimelysin (ECP 32) of Serratia grimesii and protealysin of Serratia proteamaculans, characterized by both a highly specific “actinase” activity and their ability to stimulate bacterial invasion. Grimelysin cleaves the only polypeptide bond Gly42-Val43 in actin. This bond is not cleaved by any other proteases and leads to a reversible loss of actin polymerization. Similar properties were characteristic for another bacterial protease, protealysin. These properties made grimelysin and protealysin a unique tool to study the functional properties of actin. Furthermore, bacteria Serratia grimesii and Serratia proteamaculans, producing grimelysin and protealysin, invade eukaryotic cells, and the recombinant Escherichia coli expressing the grimelysin or protealysins gene become invasive. Participation of the cellular c-Src and RhoA/ROCK signaling pathways in the invasion of eukaryotic cells by S. grimesii was shown, and involvement of E-cadherin in the invasion has been suggested. Moreover, membrane vesicles produced by S. grimesii were found to contain grimelysin, penetrate into eukaryotic cells and increase the invasion of bacteria into eukaryotic cells. These data indicate that the protease is a virulence factor, and actin can be a target for the protease upon its translocation into the host cell.
Chronic Sulforaphane Administration Inhibits Resistance to the mTOR-Inhibitor Everolimus in Bladder Cancer Cells
Authors: Justin Saira , Rutz Jochen , Maxeiner Sebastian , Chun Felix K.-H. , Juengel Eva , Blaheta Roman A.
Published: June 2020

Progressive bladder cancer growth is associated with abnormal activation of the mammalian target of the rapamycin (mTOR) pathway, but treatment with an mTOR inhibitor has not been as effective as expected. Rather, resistance develops under chronic drug use, prompting many patients to lower their relapse risk by turning to natural, plant-derived products. The present study was designed to evaluate whether the natural compound, sulforaphane (SFN), combined with the mTOR inhibitor everolimus, could block the growth and proliferation of bladder cancer cells in the short- and long-term. The bladder cancer cell lines RT112, UMUC3, and TCCSUP were exposed short- (24 h) or long-term (8 weeks) to everolimus (0.5 nM) or SFN (2.5 µM) alone or in combination. Cell growth, proliferation, apoptosis, cell cycle progression, and cell cycle regulating proteins were evaluated. siRNA blockade was used to investigate the functional impact of the proteins. Short-term application of SFN and/or everolimus resulted in significant tumor growth suppression, with additive inhibition on clonogenic tumor growth. Long-term everolimus treatment resulted in resistance development characterized by continued growth, and was associated with elevated Akt-mTOR signaling and cyclin-dependent kinase (CDK)1 phosphorylation and down-regulation of p19 and p27. In contrast, SFN alone or SFN+everolimus reduced cell growth and proliferation. Akt and Rictor signaling remained low, and p19 and p27 expressions were high under combined drug treatment. Long-term exposure to SFN+everolimus also induced acetylation of the H3 and H4 histones. Phosphorylation of CDK1 was diminished, whereby down-regulation of CDK1 and its binding partner, Cyclin B, inhibited tumor growth. In conclusion, the addition of SFN to the long-term everolimus application inhibits resistance development in bladder cancer cells in vitro. Therefore, sulforaphane may hold potential for treating bladder carcinoma in patients with resistance to an mTOR inhibitor.
Regulation of Deubiquitinating Enzymes by Post-Translational Modifications
Authors: Das Tanuza , Shin Sang Chul , Song Eun Joo , Kim Eunice EunKyeong
Published: June 2020

Ubiquitination and deubiquitination play a critical role in all aspects of cellular processes, and the enzymes involved are tightly regulated by multiple factors including posttranslational modifications like most other proteins. Dysfunction or misregulation of these enzymes could have dramatic physiological consequences, sometimes leading to diseases. Therefore, it is important to have a clear understanding of these regulatory processes. Here, we have reviewed the posttranslational modifications of deubiquitinating enzymes and their consequences on the catalytic activity, stability, abundance, localization, and interaction with the partner proteins.
Manipulation of Metabolic Pathways and Its Consequences for Anti-Tumor Immunity: A Clinical Perspective
Authors: Yang Huang-Yu , Wu Chao-Yi , Powell Jonathan D. , Lu Kun-Lin
Published: June 2020

In the relatively short history of anti-tumor treatment, numerous medications have been developed against a variety of targets. Intriguingly, although many anti-tumor strategies have failed in their clinical trials, metformin, an anti-diabetic medication, demonstrated anti-tumor effects in observational studies and even showed its synergistic potential with immune checkpoint inhibitors (ICIs) in subsequent clinical studies. Looking back from bedside-to-bench, it may not be surprising that the anti-tumor effect of metformin derives largely from its ability to rewire aberrant metabolic pathways within the tumor microenvironment. As one of the most promising breakthroughs in oncology, ICIs were also found to exert their immune-stimulatory effects at least partly via rewiring metabolic pathways. These findings underscore the importance of correcting metabolic pathways to achieve sufficient anti-tumor immunity. Herein, we start by introducing the tumor microenvironment, and then we review the implications of metabolic syndrome and treatments for targeting metabolic pathways in anti-tumor therapies. We further summarize the close associations of certain aberrant metabolic pathways with impaired anti-tumor immunity and introduce the therapeutic effects of targeting these routes. Lastly, we go through the metabolic effects of ICIs and conclude an overall direction to manipulate metabolic pathways in favor of anti-tumor responses.
A Biological and Immunological Characterization of Schistosoma Japonicum Heat Shock Proteins 40 and 90α
Authors: Xu Zhipeng , Ji Minjun , Li Chen , Du Xiaofeng , Hu Wei , McManus Donald Peter , You Hong
Published: June 2020

We characterized Schistosoma japonicum HSP40 (Sjp40) and HSP90α (Sjp90α) in this study. Western blot analysis revealed both are present in soluble egg antigens and egg secretory proteins, implicating them in triggering the host immune response after secretion from eggs into host tissues. These observations were confirmed by immunolocalization showing both HSPs are located in the Reynolds’ layer within mature eggs, suggesting they are secreted by miracidia and accumulate between the envelope and the eggshell. Both HSPs are present in the musculature and parenchyma of adult males and in the vitelline cells of females; only Sjp90α is present on the tegument of adults. Sjp40 was able to enhance the expression of macrophages, dendritic cells, and eosinophilic cells in mouse liver non-parenchymal cells, whereas rSjp90α only stimulated the expression of dendritic cells. T helper 1 (Th1), Th2, and Th17 responses were increased upon rSjp40 stimulation in vitro, but rSjp90 only stimulated an increased Th17 response. Sjp40 has an important role in reducing the expression of fibrogenic gene markers in hepatic stellate cells in vitro. Overall, these findings provide new information on HSPs in S. japonicum, improving our understanding of the pathological roles they play in their interaction with host immune cells.
Developmental Differences on the Internal Reproductive Systems between the Prediapause and Prereproductive Riptortus pedestris Adults
Authors: Hafeez Abdul , Li Bei , Liu Wen , Atiq Muhammad Nauman , Wang Xiao-Ping
Published: June 2020

Riptortus pedestris (Heteroptera: Alydidae), an important crop pest, is capable of entering reproductive adult diapause under short-day photoperiods. Though the physiological aspects of adult diapause have been well studied in this species, little is known about its morphological development. In the present study, the adult females are discriminated as prediapause and prereproductive based on the absence and presence of mature oocytes in ovarioles, respectively. We also measured the morphological development of vitellarium and lateral oviduct in females, and the accessory gland, ejaculatory duct, and testes in males of both prereproductive and prediapause adults. Our results revealed that there is a clear significant difference in the reproductive development of prediapause and prereproductive insects. Moreover, the internal morphology of reproductive organs was suppressed in prediapausebugs compared to prereproductive bugs, and the insects developedthe reproductive parts as newly emerged adults. The above findings provide basic knowledge on the characterization of diapause and reproductive R. pedestris adults, which would be applicable to molecular investigations.
Large- and Small-Scale Environmental Factors Drive Distributions of Ant Mound Size Across a Latitudinal Gradient
Authors: Juhász Orsolya , Bátori Zoltán , Trigos-Peral Gema , Lőrinczi Gábor , Módra Gábor , Bóni Imola , Kiss Péter , Aguilon Dianne , Tenyér Anna , Maák István
Published: June 2020

Red wood ants are keystone species of forest ecosystems in Europe. Environmental factors and habitat characteristics affect the size of their nest mounds, an important trait being in concordance with a colony’s well-being and impact on its surroundings. In this study, we investigated the effect of large-scale (latitude and altitude) and small-scale environmental factors (e.g., characteristics of the forest) on the size of nest mounds of Formica polyctena in Central Europe. We predicted that the change in nest size is in accordance with Bergmann’s rule that states that the body size of endotherm animals increases with the higher latitude and/or altitude. We found that the size of nests increased along the latitudinal gradient in accordance with Bergmann’s rule. The irradiation was the most important factor responsible for the changes in nest size, but temperature and local factors, like the perimeter of the trees and their distance from the nest, were also involved. Considering our results, we can better understand the long-term effects and consequences of the fast-changing environmental factors on this ecologically important group. This knowledge can contribute to the planning of forest management tactics in concordance with the assurance of the long-term survival of red wood ants.
Middle Cerebral Artery Doppler Velocimetry for the Diagnosis of Twin Anemia Polycythemia Sequence: A Systematic Review
Authors: Brock Clifton O. , Bergh Eric P. , Moise Kenneth J. , Johnson Anthony , Hernandez-Andrade Edgar , Lai Dejian , Papanna Ramesha
Published: June 2020

Twin anemia polycythemia sequence (TAPS) is a rare complication of monochorionic diamniotic (MCDA) twins. Middle cerebral artery peak systolic velocity (MCA-PSV) measurements are used to screen for TAPS while fetal or neonatal hemoglobin levels are required for definitive diagnosis. We sought to perform a systematic review of the efficacy of MCA-PSV in diagnosing TAPS. Search criteria were developed using relevant terms to query the Pubmed, Embase, and SCOPUS electronic databases. Publications reporting diagnostic characteristics of MCA-PSV measurements (i.e., sensitivity, specificity or receiver operator curves) were included. Each article was assessed for bias using the Quality Assessment of Diagnostic Accuracy Studies II (QUADAS II) tool. Results were assessed for uniformity to determine whether meta-analysis was feasible. Data were presented in tabular form. Among publications, five met the inclusion criteria. QUADAS II analysis revealed that four of the publications were highly likely to have bias in multiple areas. Meta-analysis was precluded by non-uniformity between definitions of TAPS by MCA-PSV and neonatal or fetal hemoglobin levels. High-quality prospective studies with consistent definitions and ultrasound surveillance protocols are still required to determine the efficacy of MCA-PSV in diagnosing TAPS. Other ultrasound findings (e.g., placenta echogenicity discordance) may augment Doppler studies.
Outcome in Patients with Partial and Full-Thickness Cheek Defects following Free Flap Reconstruction—A Multicentric Analysis of 47 Cases
Authors: Janik Stefan , Eljazzar Rachelle , Faisal Muhammad , Grasl Stefan , Vyskocil Erich , Miles Brett A. , Brunner Markus , Seemann Rudolf , Erovic Boban M.
Published: June 2020

The objective of this study was to evaluate whether the extent of tumor resection and free flap reconstruction influences functional outcome and complications in patients with solid malignancies of the cheek. Therefore, we retrospectively assessed recipient site complications and functional outcomes in 47 patients with solid malignancies of the cheek who underwent either partial (n = 30; 63.8%) or full-thickness (n = 17; 36.2%) cheek resection with free flap reconstruction. Complications occurred in 12 (70.6%) patients after full thickness resections with creation of through-and-through defects compared to 14 (70.6%) patients with partial defects (p = 0.138). Among those 26 patients (55.3%), major recipient site complications, like development of salivary fistula or free flap loss, were observed in 10 (21.3%) and 2 (4.3%) cases, respectively, while minor complications, like wound dehiscence and local infections, were found in 14 (29.8%) and 9 (19.1%) patients. Complications were noticed particularly after reconstruction of suborbital defects (69.2%; p = 0.268), of which occurrence of salivary fistulae was the most common (46.2%; p = 0.035). Similarly, functional outcomes including oral incompetence, ectropion, and trismus were not affected by the extent of resection (p = 0.766). However, oral incompetence was higher in patients with tumors originating from the oral cavity (p = 0.020) and after the performance of mandibulectomy (p = 0.003). Overall, there was no difference in functional outcome or recipient site morbidity between tumor resections resulting in full-thickness and partial defects.
Implications of Hereditary Origin on the Immune Phenotype of Mismatch Repair-Deficient Cancers: Systematic Literature Review
Authors: Bohaumilitzky Lena , von Knebel Doeberitz Magnus , Kloor Matthias , Ahadova Aysel
Published: June 2020

Microsatellite instability (MSI) represents one of the major types of genomic instability in human cancers and is most common in colorectal cancer (CRC) and endometrial cancer (EC). MSI develops as a consequence of DNA mismatch repair (MMR) deficiency, which can occur sporadically or in the context of Lynch syndrome (LS), the most common inherited tumor syndrome. MMR deficiency triggers the accumulation of high numbers of somatic mutations in the affected cells, mostly indel mutations at microsatellite sequences. MSI tumors are among the most immunogenic human tumors and are often characterized by pronounced local immune responses. However, so far, little is known about immunological differences between sporadic and hereditary MSI tumors. Therefore, a systematic literature search was conducted to comprehensively collect data on the differences in local T cell infiltration and immune evasion mechanisms between sporadic and LS-associated MSI tumors. The vast majority of collected studies were focusing on CRC and EC. Generally, more pronounced T cell infiltration and a higher frequency of B2M mutations were reported for LS-associated compared to sporadic MSI tumors. In addition, phenotypic features associated with enhanced lymphocyte recruitment were reported to be specifically associated with hereditary MSI CRCs. The quantitative and qualitative differences clearly indicate a distinct biology of sporadic and hereditary MSI tumors. Clinically, these findings underline the need for differentiating sporadic and hereditary tumors in basic science studies and clinical trials, including trials evaluating immune checkpoint blockade therapy in MSI tumors.
The Influence of Various Smoking Categories on The Risk of Gestational Hypertension and Pre-Eclampsia
Authors: Lewandowska Małgorzata , Więckowska Barbara
Published: June 2020

The relationship between smoking and the risk of pregnancy-induced hypertension (PIH) is not clearly established. Therefore, we conducted an analysis of cigarette smoking in a Polish cohort of women, recruited in the first trimester of a single pregnancy in 2015–2016. We evaluated the women who subsequently developed PIH (n = 137) (gestational hypertension—GH (n = 113) and pre-eclampsia—PE (n = 24)), and the women who remained normotensive (n = 775). The diseases odds ratios (and 95% CI—confidence intervals) were calculated in a multivariate logistic regression. In the PIH cases (vs. normotensive women) we found more smokers (25.6% vs. 17.2%, p = 0.020) including smokers in the first trimester (14.6% vs. 4.8%, p < 0.001). The average number of cigarettes smoked daily per smokers in the first trimester was 11.2 (range 2–30), and the average number of years of smoking was 11.6 (range 2–25). The number of years of smoking was a stronger risk factor for GH and PE than the number of cigarettes/day. Compared to the women who have never smoked, smoking ever before pregnancy was associated with a higher GH risk (AOR = 1.68; p = 0.043), and with no effect on PE risk (OR = 0.97; p = 0.950). Smokers in the first trimester had a higher odds ratio of GH (AOR = 4.75; p < 0.001) and PE (OR = 2.60; p = 0.136). Quitting smoking before pregnancy (ex-smokers) was associated with a lower odds ratio of GH (AOR = 0.83; p = 0.596) and PE (OR = 0.33; p = 0.288). However, quitting smoking during pregnancy was associated with a higher risk of GH (AOR = 11.63; p < 0.0001) and PE (OR = 3.57; p = 0.238). After dissection of the cohort into pre-pregnancy body–mass index (BMI) categories, smoking in the first trimester was associated with the higher hypertension risk in underweight women (OR = 22.00, p = 0.024). Conclusions: The factors that increased the risk of GH and PE were smoking in the first trimester and (paradoxically and more strongly) smoking cessation during pregnancy. Our results suggest that women of childbearing potential should be encouraged to quit smoking before pregnancy.
Microstructural Characteristics of Interfacial Zone in Asphalt Mixture Considering the Influence of Aggregates Properties
Authors: Hu Jing , Huang Qibo , Lou Ning , Luo Sang
Published: June 2020

The interfacial zone between aggregate particles and asphalt mortar presents a significant effect on the strength of an asphalt mixture. In this paper, basalt, limestone, and diabase were selected, and the influence of these aggregates on the mechanical characteristics and microstructures of the interfacial zone was investigated. Nanoindentation was employed to measure the change law of mechanical behavior in the region of the interfacial zone, and corresponding viscoelastic parameters were deduced; microstructural morphology was observed by scanning electron microscopy, and the effect of the mineralogical components on the interfacial zone was analyzed as well. Results show that the mechanical behavior of the interfacial transition zone is complicated. The modulus and hardness of asphalt mortar decrease with the increases in the aggregate surface distance, and then keep stable after the distance is greater than 40 μm. Both the relaxation time and dissipated energy ratio of the interfacial zone affected by the different aggregate types show a similar change law. These states indicate that aggregate types have little influence on the stress dissipation of asphalt mortar. However, creep compliances that quantify the ability of the deformation resistance show a significant difference; microstructure morphologies of the interfacial zone are affected by aggregates obviously, and micro pores present a different distribution and state in the interfacial zone.
Multi-Mode Buckling Analysis of FGM Channel Section Beams
Authors: Zaczynska Monika , Kazmierczyk Filip
Published: June 2020

The interactive buckling phenomenon in thin-walled channel section beams is investigated in this paper. This study deals with medium length beams made of the step-variable functionally graded materials (FGM) which consists of aluminum and titanium layers. The interaction of local, primary and secondary global buckling mode and their effect on the load-carrying capacity is discussed. The parametric studies are performed to assess the effect of the thickness of the beam’s component, its length and position of the individual layer on the equilibrium paths. Additionally, the influence of the adhesive layer between materials was analyzed. The problem was solved using the Finite Element Method.
Development of Graphene Oxide-Based Nonedible Cottonseed Nanofluids for Power Transformers
Authors: Farade Rizwan A. , Abdul Wahab Noor Izzri , Mansour Diaa-Eldin A. , Azis Norhafiz B. , bt. Jasni Jasronita , Soudagar Manzoore Elahi M. , Siddappa Vasudevamurthy
Published: June 2020

Sustainable materials, such as vegetable oils, have become an effective alternative for liquid dielectrics in power transformers. However, currently available vegetable oils for transformer application are extracted from edible products with a negative impact on food supply. So, it is proposed in this study to develop cottonseed oil (CSO) as an electrical insulating material and cooling medium in transformers. This development is performed in two stages. The first stage is to treat CSO with tertiary butylhydroquinone (TBHQ) antioxidants in order to enhance its oxidation stability. The second and most important stage is to use the promising graphene oxide (GO) nanosheets to enhance the dielectric and thermal properties of such oil through synthesizing GO-based CSO nanofluids. Sodium dodecyl sulfate (SDS) surfactant was used as surfactant for GO nanosheets. The nanofluid synthesis process followed the two-step method. Proper characterization of GO nanosheets and prepared nanofluids was performed using various techniques to validate the structure of GO nanosheets and their stability into the prepared nanofluids. The considered weight percentages of GO nanosheets into CSO are 0.01, 0.02, 0.03 and 0.05. Dielectric and thermal properties were comprehensively evaluated. Through these evaluations, the proper weight percentage of GO nanosheets was adopted and the corresponding physical mechanisms were discussed.
Numerical Study of Concrete Mesostructure Effect on Lamb Wave Propagation
Authors: Zima Beata , Kędra Rafał
Published: June 2020

The article presents the results of the numerical investigation of Lamb wave propagation in concrete plates while taking into account the complex concrete mesostructure. Several concrete models with randomly distributed aggregates were generated with the use of the Monte Carlo method. The influence of aggregate ratio and particle size on dispersion curves representing Lamb wave modes was analyzed. The results obtained for heterogeneous concrete models were compared with theoretical results for homogeneous concrete characterized by the averaged macroscopic material parameters. The analysis indicated that not only do the averaged material parameters influence the dispersion solution, but also the amount and size of aggregate particles. The study shows that Lamb waves propagate with different velocities in homogeneous and heterogeneous models and the difference increases with aggregate ratio and particle size, which is a particularly important observation for wave-based diagnostic methods devoted to concrete structures.
Local Mechanical Properties and Microstructure of EN AW 6082 Aluminium Alloy Processed via ECAP–Conform Technique
Authors: Procházka Radek , Sláma Peter , Dlouhý Jaromír , Konopík Pavel , Trojanová Zuzanka
Published: June 2020

An ultrafine-grained EN AW 6082 aluminum alloy was prepared by continuous serve plastic deformation (i.e., thermo-mechanical equal channel angular pressing (ECAP)–Conform process). A miniaturized tensile testing technique was used for estimating local mechanical properties with the aim to reveal the inhomogeneity of elastic and plastic properties in a workpiece volume. These inhomogeneities may appear due to the irregular shear strain distribution in a Conformed wire. Miniaturized samples for tensile testing were cut from the Conformed workpiece. Elongation of miniaturized samples was measured with a 2D digital image correlation technique as the optical extensometer. Tensile test characteristics, such as the yield strength and ultimate tensile strength, were consequently compared with results of conventional and hardness tests. The microstructure of Conformed bars was studied in the cross-section perpendicular and parallel to the extrusion direction using scanning electron microscope (SEM) and electron backscatter diffraction (EBSD) analysis. The microstructure of samples exhibits pronounced inhomogeneity, which is reflected by the hardness and tensile test results. Estimated distinctions between peripheral and central parts of the Conformed wires are probably a consequence of the significant strain differences realized in the upper and bottom wire parts.
Inhibition of Skin Inflammation by Scytonemin, an Ultraviolet Sunscreen Pigment
Authors: Kang Moo Rim , Jo Sun Ah , Lee Hyunju , Yoon Yeo Dae , Kwon Joo-Hee , Yang Jeong-Wook , Choi Byeong Jo , Park Ki Hwan , Lee Myeong Youl , Lee Chang Woo , Lee Kyeong-Ryoon , Kang Jong Soon
Published: June 2020

Scytonemin is a yellow-green ultraviolet sunscreen pigment present in different genera of aquatic and terrestrial blue-green algae, including marine cyanobacteria. In the present study, the anti-inflammatory activities of scytonemin were evaluated in vitro and in vivo. Topical application of scytonemin inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear swelling in BALB/c mice. The expression of tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) was also suppressed by scytonemin treatment in the TPA-treated ear of BALB/c mice. In addition, scytonemin inhibited lipopolysaccharide (LPS)-induced production of TNF-α and nitric oxide (NO) in RAW 264.7 cells, a murine macrophage-like cell line, and the mRNA expressions of TNF-α and iNOS were also suppressed by scytonemin in LPS-stimulated RAW 264.7 cells. Further study demonstrated that LPS-induced NF-κB activity was significantly suppressed by scytonemin treatment in RAW 264.7 cells. Our results also showed that the degradation of IκBα and nuclear translocation of the p65 subunit were blocked by scytonemin in LPS-stimulated RAW 264.7 cells. Collectively, these results suggest that scytonemin inhibits skin inflammation by blocking the expression of inflammatory mediators, and the anti-inflammatory effect of scytonemin is mediated, at least in part, by down-regulation of NF-κB activity. Our results also suggest that scytonemin might be used as a multi-function skin care ingredient for UV protection and anti-inflammation.
The Important Role of Dissolved Oxygen Supply Regulated by the Hydraulic Shear Force during the Biosynthesis of Iron Hydroxysulfate Minerals
Authors: Yang Jun , Wang Rui , Wang Heru , Song Yongwei
Published: June 2020

The severity of environmental pollution from acid mine drainage (AMD) is increasingly garnering attention. In this study, the effects of hydraulic shear forces (achieved by regulating the shaking table’s rotation speed) on Fe2+ bio-oxidation and Fe3+ hydrolytic mineralization in an acidic 9K medium-FeSO4-Acidithiobacillus ferrooxidans system (simulated AMD) are investigated. Results reveal that a higher shaking speed favors a higher oxidation rate of Fe2+, whereas a very low or high shaking speed restricts the removal of Fe3+. Shaking table rotation speeds of 120–180 rpm were preferred for biomineralization treatment in the simulated AMD. As the initial concentration of Fe2+ in the system decreased from 9.67 to 0 g/L in 40 h, the dissolved O2 (DO) in the solution dropped to its lowest concentration after 20 h and then increased to its initial level between 40 and 120 h. However, the corresponding total Fe (TFe) precipitation efficiency increased with the increasing mineralization time after 40 h. The effect of O2 supply time on biomineralization revealed that DO was mainly used in Fe2+ bio-oxidation. After Fe2+ was completely oxidized by A. ferrooxidans, the precipitation efficiency of TFe was independent of the O2 supply.