bluuebunny/crossref_metadata_2025 huggingface dataset:
Anatomohistological characteristics of Meckel's diverticulum in human fetuses
Authors: Pavlovic Snezana , Zdravkovic Dejan , Stefanovic Natalija , Pavlovic Miljana , Bakic Mirjana , Zivkovic Vladimir
Published: December 2010

Background/Aim. Meckel's diverticulum (MD) is the most frequent anomaly of the small intestine. It appears after incomplete obliteration of the omphalomesenteric or viteline duct which normally obliterates and disappears by the 9th week of gestation. The majority of MD do not give rise to any clinical symptoms and are encounted either incidentally, at examination or intervention, or due to complications which may occur (obstruction, hemorrhagy, rupture), and are described in many clinical reports. The aim of the study was to find out the incidence of MD in fetuses when the development of the alimentary tract is already finished. Methods. The investigation was performed on 150 human fetuses of different sex and gastational age, using microdissection method. The cases with MD were photographed, described, their positions and dimensions were registered. The samples of MD taken for histological investigation were dyed with hematoksilin eosin method. Results. Meckel's diverticulum was found in five fetuses (three male and two female); in one case the fibrous band was found. All of them were located on animesenteric margine of the small intestine at the average distance of 92.5 mm from the ileocecal junction. They were of different shape and dimensions, but of the normal constitution of the small intestine. Conclusion. The incidence of MD was 3.3%, and 4% of all the anomalies of the intestines connected to the disappearance of the viteline duct. It was more frequent in the male, located on antimesenteric margine of the small intestine, at the destination which highly correlated to the age of the fetus. Meckel's diverticule were of different shapes and dimensions but of the typical constitution of the small intestine. .
Graves-Basedow disease and potential risk factors
Authors: Zivanovic Dusica , Sipetic Sandra , Stamenkovic-Radak Marina , Milasin Jelena
Published: December 2010

Background/Aim. Graves-Basedow disease is a common multifactorial genetic syndrome, which is determined by several genes and environmental factors. The aim of the present study was to investigate the presence of risk factors for developing Graves-Basedow disease between the groups of individuals with and without Graves-Basedow disease, and to compare the presence of risk factors between the affected individuals with or without positive family history for Graves- Basedow disease. Methods. This cross-sectional study was conducted in Cuprija (central Serbia) during a period from December 2001 to April 2002. The case group comprised 132 individuals diagnosed with Graves-Basedow disease. The control group comprised 130 subjects without any of endocrine diseases. All participants were interviewed at the Medical Center Cuprija using structural questionnaire. Data were collected on basic demographic characteristics, exposure to various chemical and physical agents, stress, smoking and family history of Graves-Basedow disease. In statistical analysis chi-square test was used. Results. The individuals with Graves-Basedow disease were statistically significantly older (above 50) (p = 0.020), exposed to stress (p = 0.024) and to physical agents (p = 0.031), and had significantly (p = 0.000) more relatives with Graves-Basedow disease than those without the disease. Among the affected individuals with positive family history of Graves-Basedow disease the number of women was significantly higher (p = 0.000), than the affected individuals without positive family history of Graves- Basedow disease. Conclusion. In our study, as in many other, gender, age, positive family history of Graves-Basedow disease and exposure to physical agents were identified as potential risk factors for the increased incidence of Graves- Basedow disease. Different risk factors are probably responsible for developing Graves-Basedow disease among the affected individuals with or without positive family history of Graves-Basedow disease. .
Dynamic pharmacotherapy: A relationship between psychotherapy and drug
Authors: Lopicic Zorka , Gajic Tomislav , Mihajlovic Goran , Stamatovic-Gajic Branka
Published: December 2010

 Psihoterapija i psihofarmakoterapija, bez obzira na nase terapijske stavove i htenja, prozimaju se uzajamno i doprinose boljitku individue. Istrazivanja, kao i klinicka praksa, pokazali su da su ove dve metode kompatibilni nacini lecenja i da uzajamno pojacavaju svoje ucinke, koji su u tim slucajevima znatno veci nego kada se primenjuje samo jedna metoda lecenja. .
Hyperbaric medicine in emergencies
Authors: Rabrenovic Milorad , Matunovic Radomir , Rabrenovic Violeta , Todorovic Veljko , Micevic Dusan , Zoranovic Uros
Published: December 2010

 Imajuci u vidu mali broj kontraindikacija, primena HBOT od velike je koristi u zbrinjavanju urgentnih stanja, narocito u kombinaciji sa hirurskim i medikamentnim procedurama. .
Surgical treatment of Arnold-Chiari malformation type I in an adult patient
Authors: Markovic Marko , Berisavac Iva , Bojovic Vladimir , Kostic Bojan , Djulejic Vuk
Published: December 2010

Background. Herniation of the cerebellar tonsils through the foramen magnum into the cervical spinal canal with obliteration of the cerebellomedullary cistern is the primary feature of Arnold-Chiari type I malformation (ACM I). It is considered to be congenital malformation, although there have been reported cases of an acquired form. Case report. We presented a female patient, 45-year old, with ACM I without syringomyelia as a rare and unusual clinical image, as well as the effect of decompressive surgery in the treatment of this malformation. The patient was admitted to the Department of Neurosurgery with clinical signs of truncal ataxia worsening during the last six years. Moderate quadriparesis with predominant lower extremity involvement and the signs of the cranial nerves damages occured during the last seven months before admission, with progressive clinical course up to the date of admission. Neurosurgical treatment that included suboccipital medial craniectomy with resection of posterior arch C1 vertebrae and C2 laminectomy resulted in a significant clinical improvement and a much better quality of life. Conclusion. Posterior craniovertebral decompression with microsurgical reduction of the cerebellar tonsils and placement of an artificial dural graft is a treatment of choice in severe forms of ACM I without syringomyelia. .
Determination of fibrin glue with antibiotics on collagen production in colon anastomosis
Authors: Stanojkovic Zoran , Stanojevic Goran , Stojanovic Miroslav , Milic Dragan , Zivic Sasa
Published: December 2010

Background/Aim. Fibrin glue is used as a matrix for local application of antibiotics. The aim of this study was to determine whether application of fibrin glue in combination with antibiotics can strengthen collagen production, prevent dehiscence of colon anastomoses due to infection, and reduce frequency of mortality and morbidity comparing to the control group and the group with fibrin glue application. Methods. The adult male Wistar rats divided into three groups were used in the experiment. The group 1 was the control one (after partial colon resection, colonic anastomoses performed were not treated), while to the group 2 and the group 3 were applied fibrin glue and fibrin glue with antibiotics (clindamycin and ceftriaxon) on the site of anastomoses, respectively. Quality of colonic anastomoses were estimated by means of determination of collagen (L-hydroxyproline) amount in the collon wall with anastomoses and histological analysis of this colon segment using light and electronic microscope on the days 5, 7 and 13 postoperatively. Results. The highest morbidity rate was registered in the group 1 (30%), then in the group 2 (13.3%) and the lowest one in the group 3 (3.33%; p < 0,05 vs group 1). Mortality rate was significantly higher in the group 1 than in the group 3 (20% and 0%, respectively; p < 0,05). In the postoperative course, the highest concentrations of collagen in the colon wall on the site of anastomoses, which was confirmed by both light and electronic microscopy, were found in the group 3. Conclusion. The application of fibrin glue with antibiotics on colon anastomoses reduces the number of dehiscence, provides good mechanical protection and shorten the time of anastomoses healing.
Acute abdomen in a patient with Mayer-Rokitansky-Kuster-Hauser syndrome
Authors: Petric Aleksandra , Stefanovic Milan , Vukomanovic Predrag , Zivadinovic Radomir , Tubic Aleksandra , Janjic Zoran
Published: December 2010

Background. Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome is a malformation of female genital tract (incidence 1 in 4000 female newborn children). It appears as a result of a disorder in the development of Millerian cannals. Etiology is unknown. Syndrome MRKH is the most frequent cause of primary amenorrhoea (90%). Patients with MRKH have a normal female phenotype, with normal pubic hairness and thelarche, and female karyotype (46XX) followed by primary amenorrhoea. Hormonal status corresponds to healthy women, where the appearance of ovarian tumors and tumors on rudiment parts of uterus is possible. Case report. We presented a case of acute abdomen in a patient with previously not diagnosed MRKH. The diagnosis was done during the operation. Small pelvis and an abdominal part were filled with torquated tumor lump, where ovaries, oviducts, uterus or something resembling rudiment of uterus were not recognized through careful examination. Furthemore, the patient had a short, dead-end vagina. Tumorectomy was done and hystopathological finding showed the presence of vascular leiomyoma. Conclusion. The diagnosis of complex syndromes, such as MRKH, can, despite modern diagnostics, be absent for non-medical and psycho-social reasons. We can expect ovarian and uterine pathology on hypoplastic structures in these patients, as well as in healthy women. Vascular leiomyoma in the patients with MRKH was not found in the available literature.
Vascular gastric anomalies as a cause of relapsing bleeding
Authors: Golubovic Gradimir , Kiurski Milosav , Spica Vladimir , Tomasevic Ratko , Pavlovic Aleksandar , Djukic Nikola
Published: December 2010

Background. Although relatively rare, gastric vascular anomalies can be recognized as a source of both chronic and acute blood loss, most often presenting as long term iron deficiency anemia, rarely as severe acute gastrointestinal bleeding. Case report. We present five patients with various gastric vascular anomalies, diagnosed during the year of 2003. in the Clinical Hospital Center Zemun. The diagnosis was based on endoscopic appearances, clinical history and characteristic histological findings. Gastric vascular anomalies presented in our review were: portal hypertensive gastropathy, gastric antral vascular ectasia, angiodysplasia, hereditary hemorrhagic telangiectasia and Dieulafoy lesion. The used treatment modalities included surgery and various endoscopic techniques (schlerotherapy, argon plasma coagulation). Conclusion. Patients presented with chronic iron deficiency anemia or acute and recurrent gastrointestinal hemorrhage should be considered as having one of gastric vascular anomalies.
Influence of the background activity region selection on the measurement of glomerular filtration rate using the Gates method
Authors: Dopudja Marija , Ajdinovic Boris , Jaukovic Ljiljana , Petrovic Marijana , Jankovic Zoran
Published: December 2010

Background/Aim. The most frequent method for the assessment of glomerular filtration rate GFR) in clinical practice is clearance of creatinine, clearance of chromium-51 radiolabelled ethylene diamine tetraacetic acid (51Cr-EDTA) and clearance of technetium-99m radiolabelled diethylene triamine pentaacetic acid (99mTc-DTPA). The Gates method for glomerular filtration rate assessment is based on distribution of 99mTc-DTPA in the kidney 2-3 minute after its applying. Calculation of GFR is corrected for the background and depth of the kidney and finally expressed as a percentage of the net injected counts. This value of GFR highly correlates with biexponential model as the most accurate method for the assessment of GFR. The aim of this study was to determine the influence of different background sites on GFR rate assessment using the Gates method. Methods. We analysed 50 patients who were divided into two groups: the group of healthy subjects (21 subjects, mean age 47.38 yrs) and the group of subjects with unilateral kidney damage (29 subjects, mean age 39.79 yrs). Three different background activity regions were chosen: a region drawn below the lower pole of each kidney, region drawn by the lateral side of each kidney and region drawn around the whole kidney. Results. Gromerular filtration rate calculated by the use of a region under the lower pole is statistically significantly higher than GFR calculated using a region by the lateral side and around the whole kidney (p < 0.0001). Glomerular filtration rate calculated using the region by the lateral side and region around the whole kidney did not show statistically significant difference (p > 0.05). Conclusion. The selection of background activity region has a significant influence on GFR rate measured by the Gates method. It is recommended to use only one method for choosing the region of background activity.
Color Doppler imaging and measurements of intraovarian and intrauterine vascularization on basal ultrasound examination in spontaneous ovulatory and anovulatory cycles
Authors: Kutlesic Ranko , Milosavljevic Mileva , Vukomanovic Predrag , Stefanovic Milan
Published: December 2010

Background/Aim. Any organs functioning directly depends on vascularization. It applies also to the uterus and ovary which go through changes of vascularization during a menstruation cycle. The aim of this investigation was to determine differences in intrauterine and ovarian stromal arterioral blood flow on basal ultrasound examination (day 2-4) between spontaneous ovulatory and anovulatory cycles. Methods. This prospective clinical investigation included 205 patients divided into two groups: with ovulatory and with anovulatory cycles. Results. Resistance to ovarian arterioral stromal blood flow was significantly lower in the patients with ovulatory cycles (pulsatile index - PI 0.97 ? 0.4 vs 1.93 ? 1.37; p = 0.001737; and (resistance index - RI 0.55 ? 0.12 vs 0.68 ? 0.14; p = 0.040033). There were no statistically significant differences in arcuate arterioral blood flow in the pateints with ovulatory and anovulatory cycles (PI 1.21 ? 0.34 vs 61 ? 0,61 p = 0.136161 and RI 0.64 ? 0.11 vs 0.74 ? 0.07; p = 0.136649). The patients with ovulatory cycles had lower uterine radial arterioral blood flow than the patients with anovulatory cycles (PI 1.001 ? 0.22 vs 1.61 ? 0.23 p = 0.007501 and RI 0.55 ? 0.08 vs 0.71 ? 0.12; p = 0,0460113). The patients with ovulatory cycles had lower subendometrial arterioral blood flow resistance (PI 0.69 ? 0.19 vs 1.385?0.09; p = 0.00622 and RI 0.44 ? 0.09 vs 0.65 ? 0.02; p = 0.027458). Conclusion. Color Doppler ultrasuond imaging and measurements of intrauterine and ovarian stromal arterioral blood flow on basal ultrasound examination (day 2-4), showed lower resistance to blood flow in ovulatory than in anovulatory cycles.
Therapeutic efficacy of guided tissue regeneration and connective tissue autotransplants with periosteum in the management of gingival recession
Authors: Jovicic Bojan , Lazic Zoran , Nedic Milica
Published: December 2010

Background/Aim. Gingival recession progression in clinical practice as an ethiological factor of periodontal diseases, and symptoms of the disease have caused the development of various surgical procedures and techniques of the reconstruction of periodontal defects. The aim of this study was to verify efficacy of surgical procedures that include connective tissue autotransplants with periosteum and guided tissue regeneration for the treatment of gingival recession. Methods. The study included 20 teet with gingival recession, M?ller class II and III. Ten teeth with gingival recession were treated with resorptive membrane and coronary guided surgical flap (GTR group). On the contralateral side 10 teeth with gingival recession were treated with connective tissue autotransplants with periosteum in combination with coronary guided surgical flap (TVT group). We measured the degree of epithelial attachment (DEA), width of subgingival curettage (WGC) and vertical deepness of recession (VDR). For statistical significance we used Student's ttest. Results. The study revealed statistical significance in reducing VDR by both used treatments. Root deepness in GTR and TVT group was 63.5%, and 90%, respectively. With both surgical techniques we achieved coronary dislocation of the epithelial attachment, larger zone of gingival curettage, and better oral hygiene. Conclusion. Current surgical techniques are effective in the regeneration of deep periodontal spaces and the treatment of gingival recession. Significantly better results were achieved with the used coronary guided surgical flap than with guided tissue regeneration.
Inflammatory myofibroblastic tumor of the larynx
Authors: Mijovic Zaklina , Mihailovic Dragan , Kostov Milos
Published: December 2010

Background/Aim. Inflammatory myofibroblastic tumor (IMT) of the larynx is a rare borderline lesion composed of myofibroblastic and inflammatory cells. Case report. We presented a case of 77-year-old male with hoarseness for about three months. Laryngoscopy showed a polypoid tumor originated from the anterior commissure with glottic projection. Grossly, the tumor was 22 ? 15 mm, whitish, oval nodus with firm consistency. Histologically, the lesion was composed of spindled to stellate cells arranged in a fascicular to storiform pattern and inflammatory cells: lymphocytes, histiocytes, plasma cells and neutrophils. The stroma was fibromyxoid and collagenous. Immunohistochemically, tumor cells showed strong positivity for actin and vimentin and negativity for cytokeratin, CD34, S100 and desmin. The proliferation (Ki67) was less than 10%. Conclusion. Inflammatory myofibroblastic tumor may be mistaken as a malignancy and the differential diagnosis can be difficult without immunohistochemistry. It is critical to recognize this uncommon entity to avoid unnecessary aggressive treatment.
Periodontal condition in diabetics in Belgrade
Authors: Andjelski-Radicevic Biljana , Zelic Obrad , Mirkovic Silvija , Todorovic Tatjana
Published: December 2010

Background/Aim. Diabetes mellitus (DM) as a complex metabolic disease influences functioning of numerous organs. Therefore, frequent diabetic complication is chronic periodontitis. The aim of the study was to investigate the influence of various risk factors, like age, sex, duration of DM, oral hygiene, on oral manifestations of diabetes. Methods. The group of diabetics included 52 adult patients, 18-79-year of age, both sexes (33 females, 19 males) out of which 35 patients were with diabetes mellitus type I and 17 with type II. Mean age of the patients was 54.6 years and the mean duration of diabetes was 11.1 years. The controls consited of 67 volunteers from the city of Belgrade, 19-24-year of age, both sexes (47 females, 20 males) with no paradonthopathy and non-systemic diseases. Estimation of periodontal status of the patients was performed by measuring dental plaque index, gingival index and periodontal pockets' depth. Results. The results suggest an increased incidence and severity of periodontitis in diabetic patients. It was established that duration of diabetes, patients' age and bad oral hygiene had a negative influence on status of remained teeth in diabetics, while the sex had no influence on parameters monitored. Conclusion. Indices of oral hygiene level and periodontal status (dental plaque index, gingival index and periodontal pockets' depth) were significantly worsened in patients with diabetes. Negative influence on remained teeth had patients' age (>50 years), duration of diabetes (> 20 years) and bad oral hygiene. About half of the patients had a total or partial bridge suggesting that periodontitis is significant complication of diabetes mellitus.
Etiology of ischemic stroke among young adults of Serbia
Authors: Jovanovic Dejana , Beslac-Bumbasirevic Ljiljana , Raicevic Ranko , Zidverc-Trajkovic Jasna , Ercegovac Marko
Published: December 2010

Background/Aim. Etiology of ischemic stroke (IS) among young adults varies among countries. The aim of the study was to investigate the causes and risk factors of IS in the young adults of Serbia. Methods. A total of 865 patients with IS, aged 15 to 45 years, were treated throughout the period 1989-2005. Etiologic diagnostic tests were performed on the patient by the patient basis and according to their availability at the time of investigation. The most likely cause of stroke was categorized according to the TOAST (Trial of ORG 10172 in Acute Stroke Treatment) criteria. Results. There were 486 men and 379 women, with 19% of the patients ? 30 years old. Large artery arteriosclerosis and small artery disease were confirmed in 14% of the patients, and embolism and other determined causes in 20%. Undetermined causes made up 32% of the patients, mostly those (26%) with incomplete investigations. Smoking (37%), hypertension (35%) and hyperlipidemia (35%) were the most common risk factors. Rheumatic heart diseases and prosthetic valves were the most common causes of IS. Arterial dissections and coagulation inhibitors deficiency were detected in a small number of patients. Conclusion. Etiology of IS among Serbian young adults shares characteristics of those in both western and less developed countries.
Immunohistohemical evidences of pregnancy in uterine curettage tissue by the use of a double immunocytochemical staining technique using cytokeratin 7 and vimentin antibodies
Authors: Trenkic Milan , Basic Marin , Milentijevic Maja , Petrovic Aleksandar , Zivkovic Vesna , Lazarevic Viktor
Published: December 2010

Background/Aim. Usual histopatological diagnosis of intrauterine pregnancy is made by demonstration of chorionic villi, but in the curettage tissue from intrauterine miscarriage they may not be present in all cases. The use of monoclonal antibody against cytokeratin as a sensitive and reliable marker for the morphologic discrimination between invasive trophoblastic (IT) cells and decidual cells has been well established. The aim of this study was to determine the presence of pregnancy in endometrial curettings when chorionic villi are absent from patients suspected of intrauterine pregnancy. Methods. Twenty cases of endometrial tissue specimens were investigated for cytokeratin and vimentin expression by a double immunostaining for detection of IT cells. Results. Out of the total number of cases (20) 17 cases expressed cytokeratin 7 positive IT cells, that are an evidence of pregnancy. Conclusion. The obtained results indicated, that double immunohistochemical demonstration of cytokeratin and vimentin is useful for identifying pregnancy in all chorionic villi-negative cases.
Effects of perineural steroid injections on median nerve conduction during the carpal tunnel release
Authors: Stepic Nenad , Novakovic Marjan , Martic Vesna , Peric Dusan
Published: December 2010

Background/Aim. The treatment outcome of the median nerve compressive neuropathy in the carpal zone due to carpal tunnel syndrome (CTS) is represented by recovering the nerves sensibility, conductivity, condition and strength. Perineural application of betamethasone during the surgical decompression might result in faster recovery of compressed median nerve's conduction speed. Methods. In this study 40 patients with CTS were randomly divided in the two groups. In the first group (n = 20) we performed the surgical decompression of the median nerve by the open release of the carpal tunnel, and in the second group (n = 20) we applicated a perineural injection of 1 ml of betamethason immediately after the surgical decompression. We performed the electrodiagnostic (ED) examinations 7, 30 and 90 days after the surgery, and measured the conduction speed of the median nerve in the carpal tunnel zone and the sensitivity conduction speed of the median nerve. Results. Significant differences in examined ED respective variable values in different time intervals were obtained. At the final measurements, 90 days after the surgical procedure, both groups evidenced a full recovery of the conduction speed in the carpal tunnel with statistically significant better results in the second group of the patients (t = -2.116; p = 0.043). Conclusion. Intraoperative application of the corticosteroid injection during the surgical decompression results in faster regaining of conduction speed of the median nerve.
Ebstein's anomaly as a cause of paroxysmal atrial fibrillation
Authors: Damjanovic Miodrag , Djordjevic-Radojkovic Danijela , Perisic Zoran , Apostolovic Svetlana , Koracevic Goran , Pavlovic Milan , Tomasevic Miloje , Jankovic Ruzica
Published: December 2010

Background. Ebstein's anomaly is characterized by a displacement of the tricuspid valve toward apex, because of anomalous attachment of the tricuspid leaflets. There are type B of Wolff-Parkinson-White (WPW) syndrome and paroxysmal arrhythmias in more than a half of all patients. Case report. We presented a female, 32-year old, with frequent paroxysms of atrial fibrillation. After conversion of rhythm an ECG showed WPW syndrome. Echocardiographic examination discovered normal size of the left cardiac chambers with paradoxical ventricular septal motion. The right ventricle was very small because of its atrialization. The origin of the tricuspid valve was 20 mm closer to apex of the right ventricle than the origin of the mitral valve. Electrophysiological examination showed a posterolateral right accesorial pathway. Atrial fibrillation was induced very easily in electrophysiological laboratory and a successful ablation of accessorial pathway was made. There were no WPW syndrome and paroxysms of atrial fibrillation after that. Conclusion. Ebstein's anomaly is one of the reasons of paroxysmal atrial fibrillation, especially in young persons with WPW syndrome.
Matrix metalloproteinases (MMP-1, -8, -13) in chronic periapical lesions
Authors: Andonovska Biljana , Dimova Cena , Panov Saso
Published: December 2010

Background/Aim. Matrix metalloproteinases (MMPs) are proteolytic enzymes capable of degrading almost all extracellular matrix and basement membrane components in many destructive pathological processes, such as chronic inflammation and bone-destructive lesions. The aim of this study was to determinate the correlation between concentration of collagenases (MMP-1, -8, -13) in chronic periapical lesions and their dimension calculated with software predilection through X-ray. Metods. Chronic periapical tissues were collected by periapical surgery from 60 teeth with clinically and radiographically verified different chronic periapical lesions (20 granulomas, 20 diffuse periapical lesions, 10 cysts). Ten normal pulps used as controls were obtained by extirpation of the pulp of impacted third molars after their surgery. For rapid analysis of MMP-1, -8, -13 collagenase activities in the examined material Chemicon Collagenase Activity Assay Kit were used. From the X-ray trough software predilection (Image Tool3 Program) of the volume of chronic periapical tissue, correlation between concentration of MMPs in the periapical lesions and their dimension was confirmed. Results. Different concentrations of collagenases (MMP-1, -8 and -13) in chronic periapical process from different inflammation types showed different activity of MMPs. The obtained results showed the highest values of collagenases concentration (MMP-1, -8, -13) in chronic diffuse lesions (5.39 ng/ml). Low values of concentration of MMPs accompanied less serious lesions, whereas chronical periapical lesions of large dimension had high concentration of MMPs, which was proportional to progression of the lesion and destruction of bone tissue. Conclusions. This study confirmed the destructive role of collagenases (MMP-1, -8 and -13) in inflammation process, which directly depends on the concentration of MMPs in pathologically changed tissue.
Cerebrospinal fluid amyloid beta and tau protein: Biomarkers for Alzheimer's disease
Authors: Mandic Gorana , Markovic Ivanka , Ostojic Marija , Stojkovic Tanja , Misirlic-Dencic Sonja , Zivanovic-Radnic Tatjana , Stefanovic Rodoljub , Bumbasirevic Marko , Stefanova Elka , Kostic Vladimir
Published: December 2010

Background/Aim. Introduction of acetylcholine esterase inhibitors as a symptomatic treatment of Alzheimer's disease (AD) has additionally highlighted the importance of diagnostic markers in cerebrospinal fluid (CSF) for early AD diagnosis: low level of 42 amino acid form of amyloid-? peptide (A?42), and levels of tau protein (T-tau) and phosphorylated tau protein (P-tau). The aim of this study was to diagnostic potential of CSF biomarkers T-tau, P-tau and A?42 as biochemical markers for AD. Methods. Lumbar puncture was performed in 63 patients with AD and 26 control subjects who passed orthopedic surgery. The Innotest, ELISA sandwich test (Innogenetics - Belgium) was used for measuring the levels of T-tau, P-tau and A?42. Results. The patients and the control group did not differ in age, education and sex. Mean levels of CSF T-tau and P-tau were significantly higher in the patients with AD (p < 0.001) compared to the control group, in contrast to significantely lower CSF A?42 in AD group (p < 0.001). A significant progressive decrease of A?42, as well as significant progressive increase of T-tau and P-tau was found among AD subgroups (according to MMSE staging) and controls. Conclusion. The obtained results suggest that these biomarkers may be supportive in the diagnosis of AD, especially in the early course of the disease and could be used in the routine clinical practice considering the approaching target therapeutics.
Pulse low-intensity electromagnetic field as prophylaxis of heterotopic ossification in patients with traumatic spinal cord injury
Authors: Djurovic Aleksandar , Miljkovic Dejan , Brdareski Zorica , Plavsic Aleksandra , Jevtic Miodrag
Published: December 2010

Background/Aim. Heterotopic ossification (HO) is an important complication of head and spinal cord injuries (SCI). Pulse low-intensity electromagnetic field (PLIMF) therapy increases blood flow to an area of pain or inflammation, bringing more oxygen to that area and helps to remove toxic substances. The aim of this study was to determine the effect of PLIMF as prophylaxis of HO in patients with SCI. Methods. This prospective random control clinical study included 29 patients with traumatic SCI. The patients were randomly divided into experimental (n = 14) and control group (n = 15). The patients in the experimental group, besides exercise and range of motion therapy, were treated by PLIMF of the following characteristics: induction of 10 mT, frequency of 25 Hz and duration of 30 min. Pulse low-intensity electromagnetic field therapy started in the 7th week after the injury and lasted 4 weeks. The presence or absence of HO around the patients hips we checked by a plane radiography and Brookers classification. Functional capabilities and motor impairment were checked by Functional Independent Measure (FIM), Barthel index and American Spinal Injury Association (ASIA) impairment class. Statistic analysis included Kolmogorov-Smirnov test, Shapiro-Wilk test, Mann Whitney Exact test, Exact Wilcoxon signed rank test and Fischer Exact test. Statistical significance was set up to p < 0.05. Results. At the end of the treatment no patient from the experimental group had HO. In the control group, five patients (33.3%) had HO. At the end of the treatment the majority of the patients from the experimental group (57.14%) moved from ASIA-A to ASIA-B class. Conclusion. Pulse low-intensity electromagnetic field therapy could help as prophylaxis of HO in patients with traumatic SCI.
Symptoms, physical findings and bronchial hypersensitivity in patients with bronchial asthma and normal spirometry
Authors: Acimovic Slobodan , Plavec Goran , Tomic Ilija , Karlicic Vukoica , Acimovic Svetlana , Vukovic Jelena , Loncarevic Olivera
Published: December 2010

Background/Aim. The diagnosis of bronchial asthma, a chronic inflammatory disease of the respiratory tract, is made on the basis of anamnesis, pathologic auscultatory findings of the lungs, lung function disturbances, skin tests, as well as the basic indices of immunologic condition in bronchial trunk. The aim of the study was to find out correlation of objective indices of the disease and than relation with the symptoms in the patients with bronchial asthma. Methods. The study included 60 young male non smokers with long lasting symptoms of bronchial asthma including shortness of breath, wheezing, hard breathing, nonproductive or productive cough, weakness and night hard breathing. There were no symptoms of respiratory infection over the past two months and lung radiography and spirometry were normal. Based on the results of nonspecific bronchoprovocative test two groups of the patients were formed, group I (n = 30) with positive histamine test (average value of the inhaled histamine concentration with FEV1 drop by 20% in regard with the initial value (PC20) = 2.99 ? 0.51 mg/ml of histamine) and group II (n = 30) with negative histamine test (PC20(a) = 14.58 ? 6.34 mg/ml of histamine). Results. The obtained spirometry results revealed a statistically significant difference in values of FEV1 between groups: I group - FEV1 = 93.2%; II group - FEV1 = 101.8%; (p < 0.05, Wilcoxon test), although all the FEV1 values were normal. Regarding the presence of the most common symptoms there was not statistically significant difference between the groups (p > 0. 05, chisquare test). Pathologic auscultatory lung findings were found in 73.4% of the patients in the group I and 27.5% of the patients in the group II. There was statistically significant difference (p < 0.05, chi-squared test). A positive correlation between the degree of hypersensitivity and lung physical findings was confirmed (p < 0.05 Spearman's rho), but there was no correlation with FEV1 values. Conclusion. There is a correlation with lung pathologic physical findings, lower values of FEV1 (in a range of normal values) and the degree of nonspecific bronchial sensitivity as objective indices of activity of bronchial asthma. There is no correlation of these parameters with patient's symptoms as subjective indices of bronchial asthma.
Determination of sensitivity to antibiotics of Campilobacter jejuni and Campilobacter coli isolated from human feces
Authors: Tambur Zoran , Miljkovic-Selimovic Biljana , Bokonjic Dubravko
Published: December 2010

Background/Aim. One of the most important bacterial zoonosis is campylobacteriosis. Human disease is mostly caused by thermophilic Campylobacter spp: Campylobacter jejuni (C. jejuni), Campylobacter coli (C. coli), Campylobacter lari (C. lari) and Campylobacter upsaliensis (C. upsaliensis). Campylobacteriosis is a mild and self-healing disorder. In patients with more severe and prolonged forms, an antibiotic treatment is recommended. Recommended drugs are erythromycin, ciprofloxacin, tetracyclin, chloramphenicol and ampicillin. Lately, an increase of Campylobacter genus resistance to antibiotics mostly used in therapy is an annoying evidence. The rise coincided with the beginning of antibiotic use, especially quinolones, in veterinary medicine. The aim of the study was to isolate and identify thermophilic Campylobacter spp. from human feces and to determine their sensitivity to antibiotics and hemotherapeutics mostly used in campylobacteriosis treatment. Methods. Sensitivity to erythromycin, tetracyclin, ampicillin, chloramphenicol and ciprofloxacin of 24 strains of Campylobacter spp. isolated from humans was investigated by E-test. Results. Seventeen C. jejuni and seven C. coli strains were investigated. Six (25%) out of 24 C. jejuni and C. coli investigated strains were resistant to ampicillin and seven (29.2%) were resistant to tetracycline. Twelve (50%) C. jejuni and C. coli investigated strains were resistant to ciprofloxacin. C. jejuni was more resistant (52.9%). Four (23.5%) C. jejuni strains were resistant to erythromycin and 11.7% to choramphenicol. None of C. coli strains were resistant to both chloramphenicol and erytromycin. Conclusons. Testing sensitivity to erythromycin, tetracycline, ampicillin, chloramphenicol and ciprofloxacin demonstrated a rather high resistance frequency of C. jejuni and C. coli strains isolated from humans. C. jejuni strains were more resistant than those of C. coli.
Results of modern tuboperitoneal infertility treatment
Authors: Turjacanin-Pantelic Drenka , Bojovic-Jovic Dragana , Arsic Biljana , Garalejic Eliana
Published: December 2010

Background/Aim. A modern approach to surgical treatment of tuboperitoneal infertility is based on laporascopic techniques. The aim of this study was to compare results of tuboperitoneal infertility treatment by the use of laparoscopy and classical laparotomy. Methods. A retrospectiveprospective study on 66 women treated operatively form tuboperitoneal infertility was performed. Data from patient's anamnesis and those related to the surgical treatment results, obtained by the use of an inquiry, were used in retrospective and prospective analysis, respectively. Chi-square test was used in statistical analysis. P value < 0.05 was considered significant. Results. Classical laparotomy was used on 34 women in a period from 1996 to 1997, while 32 women were operated laparoscopically in a period from 1999 to 2000. The results were as follows: a total number of conceived women was 16 (24%), seven in the group I (20.6%) and nine in the group II (28.1%); 13 women were with one pregnancy, six in the group I (17.6%) and seven in the group II (22%). Twice pregnant were three women, one in the group I (2.9%) and two in the group II (6.2%). The resulting pregnancies were: five women with abortion spontaneous, two in the group I (5.9%) and three in the group II (9.4%); two women with extrauterine pregnancy in the group I (5.9%); three with pretemporal birth, one in the group I (2.9%) and two in the group II (6.2%), while six women were with the temporal birth, two in the group I (5.9%) and four in the group II (12.5%). Statistical analysis showed that there was no significant difference in the results between these two groups. Conclusion. Surgical treatment of tubeperitoneal infertility, regardless of the used methods (classical laparotomy or laparoscopy) was successful in a great number of women. These methods have a great advantage over in vitro fertilization, and they should not be ignored.
Kimura's disease in a young Balkan male
Authors: Jovicic-Milentijevic Maja , Basic Marin , Petrovic Aleksandar
Published: December 2010

Introduction. Kimura's disease is a rare, chronic inflammatory disorder of unknown cause, mainly seen in young Asian men. To our knowledge it has not been reported previously in persons from the Balkan countries. Case report. We presented a 15-year-old male with Kimura's disease manifested as chronic left neck mass. The diagnosis was based on the histopathological findings of the excised lesion. Peripheral blood eosinophilia and raised serum Immunoglobulin E (IgE) level supported the diagnosis. Conclusion. The presented patient confirmed the fact that Kimura's disease could occur in different ethnic groups. Histopathological examination, should be performed prior to making the definitive diagnosis.
Anatomic and histological characteristics of vagina reconstructed by McIndoe method
Authors: Kozarski Jefta , Vesanovic Svetlana , Bogdanovic Zoran
Published: December 2010

Background/Aim. Congenital absence of vagina is known from ancient times of Greek. According to the literature data, incidence is 1/4 000 to 1/20 000. Treatment of this anomaly includes non-operative and operative procedures. McIndoe procedure uses split skin graft by Thiersch. The aim of this study was to establish anatomic and histological characteristics of vagina reconstructed by McIndoe method in Mayer K?ster-Rockitansky Hauser (MKRH) syndrome and compare them with normal vagina. Methods. The study included 21 patients of 18 and more years with congenital anomaly known as aplasio vaginae within the Mayer K?ster-Rockitansky Hauser syndrome. The patients were operated on by the plastic surgeon using the McIndoe method. The study was a retrospective review of the data from the history of the disease, objective and gynecological examination and cytological analysis of native preparations of vaginal stain (Papanicolau). Comparatively, 21 females of 18 and more years with normal vaginas were also studied. All the subjects were divided into the groups R (reconstructed) and C (control) and the subgroups according to age up to 30 years (1 R, 1C), from 30 to 50 (2R, 2C), and over 50 (3R, 3C). Statistical data processing was performed by using the Student's t-test and Mann-Writney U-test. A value of p < 0.05 was considered statistically significant. Results. The results show that there are differences in the depth and the wideness of reconstructed vagina, but the obtained values are still in the range of normal ones. Cytological differences between a reconstructed and the normal vagina were found. Conclusion. A reconstructed vagina is smaller than the normal one regarding depth and width, but within the range of normal values. A split skin graft used in the reconstruction, keeps its own cytological, i.e. histological and, so, biological characteristics.
Our first experiences in applying an original method for removal of ABO-isoagglutinins in ABO-incompatible kidney recipients
Authors: Ignjatovic Ljiljana , Kovacevic Zoran , Jovanovic Dragan , Vavic Neven , Paunic Zoran , Radojevic Milorad , Rabrenovic Violeta , Obrencevic Katarina , Mijuskovic Mirjana , Draskovic-Pavlovic Biljana , Ostojic Gordana , Balint Bela , Bokonjic Dubravko
Published: December 2010

Background/Aim. Due to improved methods for removal of ABO isoagglutinins and novel immunosuppressive protocols, short and long term outcome in blood group incompatible is similar to blood group compatible kidney transplantation. The aim of this study was to determine the efficacy of our original method for removal of ABO isoagglutinins from the blood in ABO-incompatible kidney allograft recipients. Method. Between 2006 and 2008 twelve patients were transplanted from ABO incompatible living donors. Titers of ABO isoagglutinins were 4-128 (IgG). Immunosuppressive therapy started 14 days before kidney transplantation with rituximab, followed by a triple therapy (prednisone + tacrolimus + mycophenolate mofetil) and the first plasma exchange (PE) procedure, in which one plasma volume was substituted with albumin and saline on day 7 before transplantation. For selective extracorporeal immunoadsorption, the removed plasma was mixed with donor blood type filtered red blood cells, centrifuged and the supernatant separated and preserved. In the next PE procedure, the removed plasma was replaced with immunoadsorbed plasma, and so on. Titers of ABO agglutinins, renal allograft function and survival were followed-up. Results. The pre-transplant treatment consisting of 1-5 PE procedures and immunosuppressive therapy resulted in target ABO agglutinins titers below 4. During a 10-24 month follow-up three patients had an early acute rejection, one patient acute rejection and hemolytic anemia, two patients surgical complications and one of them lost his graft. In the post-transplant period, the titers of ABO antibodies remained below 4. All the patients had stable kidney allograft function with mean serum creatinine ?SD of 129 ? 45 ?mol/l at the end of the study. Conclusion. Our method for removal of ABO antibodies was effective in a limited series of patients and short-term follow-up.
Clinical efficacy of ampicillin in treatment of acute odontogenic abscess
Authors: Matijevic Stevo , Lazic Zoran , Nonkovic Zorka
Published: December 2010

Background/Aim. Antibiotics choice and the duration of their application in the therapy of acute odontogenic abscess is considered to be controversial. The aim of this study was to investigate the clinical efficacy of ampicillin in treatment of acute odontogenic abscess and to assess the antimicrobial susceptibility of the isolated bacteria in early phase of abscess development. Methods. This study included 60 patients with acute odontogenic abscess who were surgically treated (extraction of teeth and/or abscess incision) divided into two groups, ampicillin group and surgical group (without antibiotic treatment). Results. In the ampicillin group of patients treatment lasted on the average 4.67 days, while in the surgical group 6.17 days. A total of 78 bacterial strains were isolated from 60 patients. The most often bacteria were found to be Gram-positive facultative anaerobs (68/78). The most common bacteria isolated were Viridans streptococci (43/78). Susceptibility of isolated bacteria to ampicillin were 70.5%. Conclusion. Peroral use of ampicillin, after surgical treatment in an early phase of dentoalveolar abscess development, statistically significantly reduced the time of clinical symptoms of acute odontogenic abscess in comparison to surgical treatment only. The isolated bacterial strains in an early phase of dentoalveolar abscess development showed a high sensitivity to ampicillin.
K-ras mutation predictive significance in platinum based chemotherapeutic protocols in patients with advanced non-small cell lung cancer
Authors: Cvetkovic Gordana , Plavec Goran , Tomic Ilija , Ilic Vesna , Magic Zvonko , Tatomirovic Zeljka , Novkovic Dobrivoje , Milic Rade , Karlicic Vukojica
Published: December 2010

Beckground/Aim. K-ras oncogene is mutated in about 20% of lung cancer. The purpose of this study was to investigate the predictive significance for therapeutic response of K-ras mutations in advanced non-small cell lung cancer (NSCLC) patients. Methods. Bronchial aspirate samples were assessed prior to platinum-based chemotherapy start in 39 patients with stage IIIb or IV NSCLC. K-ras mutations at codons 12 and 13 were analyzed by single strand conformation polymorphisam (SSCP) and allele specific oligonucleozide hybridisation of polymerase chain reaction (PCR) of the patient's DNA present in bronchial aspirate. After two cycles of chemotherapy the patients were subjected to response evaluation. Results. Of 39 patients 10 (25.5%) demonstrated K-ras mutations, while 29 (74.4%) patients had not. There were no significant differences between these two groups of patients with respect to baseline patient characteristics. Partial response to the therapy had 16 (41%), no changes 14 (36%), and progressive disease 9 (23%) patients. There was a tendency to higher response rate for patients without K-ras mutations versus those with mutations, but not statistically significant (p = 0.14). Conclusion. There was no significant predictive value for therapeutic response of K-ras mutations for advanced non-small cell lung cancer.