bluuebunny/crossref_metadata_2025 huggingface dataset:
Physical Performance and Skeletal Muscle Transcriptional Adaptations Are Not Impacted by Exercise Training Frequency in Mice with Lower Extremity Peripheral Artery Disease
Authors: Lavier Jessica , Bouzourène Karima , Millet Grégoire P. , Mazzolai Lucia , Pellegrin Maxime
Published: April 2023

Exercise training is an important therapeutic strategy for lower extremity peripheral artery disease (PAD). However, the effects of different exercise frequency on physiological adaptations remain unknown. Thus, this study compared the effects of a 7-week moderate-intensity aerobic training performed either three or five times/week on skeletal muscle gene expression and physical performance in mice with PAD. Hypercholesterolemic male ApoE-deficient mice were subjected to unilateral iliac artery ligation and randomly assigned to sedentary or exercise training regimens either three or five times/week. Physical performance was assessed using a treadmill test to exhaustion. Expression of genes related to glucose and lipid metabolism, mitochondrial biogenesis, muscle fiber-type, angiogenesis, and inflammation was analyzed in non-ischemic and ischemic gastrocnemius muscles by real-time polymerase chain reaction. Physical performance was improved to the same extent in both exercise groups. For gene expression patterns, no statistical differences were observed between three or five times/week exercised mice, both in the non-ischemic and ischemic muscles. Our data show that exercising three to five times a week induces similar beneficial effects on performance. Those results are associated with muscular adaptations that remain identical between the two frequencies.
Is the Novel Slot Blot a Useful Method for Quantification of Intracellular Advanced Glycation End-Products?
Authors: Takata Takanobu
Published: April 2023

Various types of advanced glycation end-products (AGEs) have been identified and studied. I have reported a novel slot blot analysis to quantify two types of AGEs, glyceraldehyde-derived AGEs, also called toxic AGEs (TAGE), and 1,5-anhydro-D-fructose AGEs. The traditional slot blot method has been used for the detection and quantification of RNA, DNA, and proteins since around 1980 and is one of the more commonly used analog technologies to date. However, the novel slot blot analysis has been used to quantify AGEs from 2017 to 2022. Its characteristics include (i) use of a lysis buffer containing tris-(hydroxymethyl)-aminomethane, urea, thiourea, and 3-[3-(cholamidopropyl)-dimetyl-ammonio]-1-propane sulfonate (a lysis buffer with a composition similar to that used in two-dimensional gel electrophoresis-based proteomics analysis); (ii) probing of AGE-modified bovine serum albumin (e.g., standard AGE aliquots); and (iii) use of polyvinylidene difluoride membranes. In this review, the previously used quantification methods of slot blot, western blot, immunostaining, enzyme-linked immunosorbent assay, gas chromatography–mass spectrometry (MS), matrix-associated laser desorption/ionization–MS, and liquid chromatography–electrospray ionization–MS are described. Lastly, the advantages and disadvantages of the novel slot blot compared to the above methods are discussed.
A Machine-Learning Approach to Target Clinical and Biological Features Associated with Sarcopenia: Findings from Northern and Southern Italian Aging Populations
Authors: Zupo Roberta , Moroni Alessia , Castellana Fabio , Gasparri Clara , Catino Feliciana , Lampignano Luisa , Perna Simone , Clodoveo Maria Lisa , Sardone Rodolfo , Rondanelli Mariangela
Published: April 2023

Epidemiological and public health resonance of sarcopenia in late life requires further research to identify better clinical markers useful for seeking proper care strategies in preventive medicine settings. Using a machine-learning approach, a search for clinical and fluid markers most associated with sarcopenia was carried out across older populations from northern and southern Italy. A dataset of adults >65 years of age (n = 1971) made up of clinical records and fluid markers from either a clinical-based subset from northern Italy (Pavia) and a population-based subset from southern Italy (Apulia) was employed (n = 1312 and n = 659, respectively). Body composition data obtained by dual-energy X-ray absorptiometry (DXA) were used for the diagnosis of sarcopenia, given by the presence of either low muscle mass (i.e., an SMI < 7.0 kg/m2 for males or <5.5 kg/m2 for females) and of low muscle strength (i.e., an HGS < 27 kg for males or <16 kg for females) or low physical performance (i.e., an SPPB ≤ 8), according to the EWGSOP2 panel guidelines. A machine-learning feature-selection approach, the random forest (RF), was used to identify the most predictive features of sarcopenia in the whole dataset, considering every possible interaction among variables and taking into account nonlinear relationships that classical models could not evaluate. Then, a logistic regression was performed for comparative purposes. Leading variables of association to sarcopenia overlapped in the two population subsets and included SMI, HGS, FFM of legs and arms, and sex. Using parametric and nonparametric whole-sample analysis to investigate the clinical variables and biological markers most associated with sarcopenia, we found that albumin, CRP, folate, and age ranked high according to RF selection, while sex, folate, and vitamin D were the most relevant according to logistics. Albumin, CRP, vitamin D, and serum folate should not be neglected in screening for sarcopenia in the aging population. Better preventive medicine settings in geriatrics are urgently needed to lessen the impact of sarcopenia on the general health, quality of life, and medical care delivery of the aging population.
Qualitative Analysis of Polyphenols in Glycerol Plant Extracts Using Untargeted Metabolomics
Authors: Nastasi Joseph Robert , Daygon Venea Dara , Kontogiorgos Vassilis , Fitzgerald Melissa A.
Published: April 2023

Glycerol is a reliable solvent for extracting polyphenols from food and waste products. There has been an increase in the application of glycerol over benchmark alcoholic solvents such as ethanol and methanol for natural product generation because of its non-toxic nature and high extraction efficiency. However, plant extracts containing a high glycerol concentration are unsuitable for mass spectrometry-based investigation utilising electrospray ionization, inhibiting the ability to analyse compounds of interest. In this investigation, a solid phase extraction protocol is outlined for removing glycerol from plant extracts containing a high concentration of glycerol and their subsequent analysis of polyphenols using ultra-performance liquid chromatography coupled with quadrupole time of flight tandem mass spectrometry. Using this method, glycerol-based extracts of Queen Garnet Plum (Prunus salicina) were investigated and compared to ethanolic extracts. Anthocyanins and flavonoids in high abundance were found in both glycerol and ethanol extracts. The polyphenol metabolome of Queen Garnet Plum was 53% polyphenol glycoside derivatives and 47% polyphenols in their aglycone forms. Furthermore, 56% of the flavonoid derivates were found to be flavonoid glycosides, and 44% were flavonoid aglycones. In addition, two flavonoid glycosides not previously found in Queen Garnet Plum were putatively identified: Quercetin-3-O-xyloside and Quercetin-3-O-rhamnoside.
Cerebrospinal Fluid Metabolomics: Pilot Study of Using Metabolomics to Assess Diet and Metabolic Interventions in Alzheimer’s Disease and Mild Cognitive Impairment
Authors: Hanson Angela J. , Banks William A. , Bettcher Lisa F. , Pepin Robert , Raftery Daniel , Navarro Sandi L. , Craft Suzanne
Published: April 2023

Brain glucose hypometabolism is an early sign of Alzheimer’s disease (AD), and interventions which offset this deficit, such as ketogenic diets, show promise as AD therapeutics. Conversely, high-fat feeding may exacerbate AD risk. We analyzed the metabolomic profile of cerebrospinal fluid (CSF) in a pilot study of older adults who underwent saline and triglyceride (TG) infusions. Older adults (12 cognitively normal (CN), age 65.3 ± 8.1, and 9 with cognitive impairment (CI), age 70.9 ± 8.6) underwent a 5 h TG or saline infusion on different days using a random crossover design; CSF was collected at the end of infusion. Aqueous metabolites were measured using a targeted mass spectroscopy (MS) platform focusing on 215 metabolites from over 35 different metabolic pathways. Data were analyzed using MetaboAnalyst 4.0 and SAS. Of the 215 targeted metabolites, 99 were detectable in CSF. Only one metabolite significantly differed by treatment: the ketone body 3-hydroxybutyrate (HBA). Post hoc analyses showed that HBA levels were associated with age and markers of metabolic syndrome and demonstrated different correlation patterns for the two treatments. When analyzed by cognitive diagnosis group, TG-induced increases in HBA were over 3 times higher for those with cognitive impairment (change score CN +9.8 uM ± 8.3, CI +32.4 ± 7.4, p = 0.0191). Interestingly, individuals with cognitive impairment had higher HBA levels after TG infusion than those with normal cognition. These results suggest that interventions that increase plasma ketones may lead to higher brain ketones in groups at risk for AD and should be confirmed in larger intervention studies.
Multi Frequency Controllable In-Band Suppressions in a Broad Bandwidth Microstrip Filter Design for 5G Wi-Fi and Satellite Communication Systems Utilizing a Quad-Mode Stub-Loaded Resonator
Authors: Zhang Guoqiang , Basit Abdul , Khan Muhammad Irshad , Daraz Amil , Saqib Najmus , Zubir Farid
Published: April 2023

The key elements used for receiving and processing signals in communication systems are the bandpass filters. Initially, a common operating mechanism was applied for the design of broadband filters, i.e., by cascading low-pass filters or high-pass filters using multiple line resonators with length quarter-half- or full-wavelength with central frequency, but using these approaches, the design topology becomes expensive and complex. The above mechanisms can be possibly overcome using a planar microstrip transmission line structure due to its simple design fabrication procedure and low cost. So, pointing out the above problems in bandpass filters such as low-cost, low insertion loss, and good out-of-band performance, this article presents a broadband filter with multifrequency suppression capability at 4.9 GHz, 8.3 GHz, and 11.5 GHz using a T-shaped shorted stub-loaded resonator with a central square ring coupled to the basic broadband filter. Initially, the C-shaped resonator is utilized for the formation of a stopband at 8.3 GHz for a satellite communication system, and then a shorted square ring resonator is added to the existing C-shaped structure for the realization of two more stopbands at 4.9 GHz and 11.5 GHz for 5G (WLAN 802.11j) communication, respectively. The overall circuit area covered with the proposed filter is 0.52 λg × 0.32 λg (λg is the wavelength of the feed lines at frequency 4.9 GHz). All the loaded stubs are folded in order to save the circuit area, which is an important requirement of next-generation wireless communication systems. The proposed filter has been analyzed using a well-known transmission line theory, even–odd-mode, and simulated with the 3D software HFSS. After the parametric analysis, some attractive features were obtained, i.e., compact structure, simple planar topology, low insertion losses of 0.4 dB over the entire band, good return loss greater than 10 dB, and independently controlled mutli stopbands, which make the proposed design unique and can be used in various wireless communication system applications. Finally, a Rogers RO-4350 substrate is selected for the fabrication of the prototype using an LPKF S63 ProtoLaser machine and then measured using a ZNB20 vector network analyzer for matching the simulated and measured results. After testing the prototype, a good agreement was found between the results.
Gut Microbiome–Brain Axis as an Explanation for the Risk of Poor Neurodevelopment Outcome in Preterm Infants with Necrotizing Enterocolitis
Authors: Xia Jason , Claud Erika C.
Published: April 2023

Necrotizing Enterocolitis (NEC) is characterized by an inflammation of intestinal tissue that primarily affects premature infants. It is the most common and devastating gastrointestinal morbidity of prematurity, but beyond intestinal morbidity, this condition has also been associated with an increased risk of neurodevelopmental delays that persist beyond infancy. Prematurity, enteral feeding, bacterial colonization, and prolonged exposure to antibiotics are all risk factors that predispose preterm infants to NEC. Interestingly, these factors are all also associated with the gut microbiome. However, whether or not there is a connection between the microbiome and the risk of neurodevelopmental delays in infants after NEC is still an emerging area of research. Furthermore, how microbes in the gut could impact a distant organ such as the brain is also poorly understood. In this review, we discuss the current understanding of NEC and the role of the gut microbiome–brain axis in neurodevelopmental outcomes after NEC. Understanding the potential role of the microbiome in neurodevelopmental outcomes is important as the microbiome is modifiable and thus offers the hope of improved therapeutic options. We highlight the progress and limitations in this field. Insights into the gut microbiome–brain axis may offer potential therapeutic approaches to improve the long-term outcomes of premature infants.
Adaption of Pseudomonas ogarae F113 to the Rhizosphere Environment—The AmrZ-FleQ Hub
Authors: Blanco-Romero Esther , Durán David , Garrido-Sanz Daniel , Redondo-Nieto Miguel , Martín Marta , Rivilla Rafael
Published: April 2023

Motility and biofilm formation are two crucial traits in the process of rhizosphere colonization by pseudomonads. The regulation of both traits requires a complex signaling network that is coordinated by the AmrZ-FleQ hub. In this review, we describe the role of this hub in the adaption to the rhizosphere. The study of the direct regulon of AmrZ and the phenotypic analyses of an amrZ mutant in Pseudomonas ogarae F113 has shown that this protein plays a crucial role in the regulation of several cellular functions, including motility, biofilm formation, iron homeostasis, and bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) turnover, controlling the synthesis of extracellular matrix components. On the other hand, FleQ is the master regulator of flagellar synthesis in P. ogarae F113 and other pseudomonads, but its implication in the regulation of multiple traits related with environmental adaption has been shown. Genomic scale studies (ChIP-Seq and RNA-Seq) have shown that in P. ogarae F113, AmrZ and FleQ are general transcription factors that regulate multiple traits. It has also been shown that there is a common regulon shared by the two transcription factors. Moreover, these studies have shown that AmrZ and FleQ form a regulatory hub that inversely regulate traits such as motility, extracellular matrix component production, and iron homeostasis. The messenger molecule c-di-GMP plays an essential role in this hub since its production is regulated by AmrZ and it is sensed by FleQ and required for its regulatory role. This regulatory hub is functional both in culture and in the rhizosphere, indicating that the AmrZ-FleQ hub is a main player of P. ogarae F113 adaption to the rhizosphere environment.
Overcoming the Limitations of CRISPR-Cas9 Systems in Saccharomyces cerevisiae: Off-Target Effects, Epigenome, and Mitochondrial Editing
Authors: Sato Genki , Kuroda Kouichi
Published: April 2023

Modification of the genome of the yeast Saccharomyces cerevisiae has great potential for application in biological research and biotechnological advancements, and the CRISPR-Cas9 system has been increasingly employed for these purposes. The CRISPR-Cas9 system enables the precise and simultaneous modification of any genomic region of the yeast to a desired sequence by altering only a 20-nucleotide sequence within the guide RNA expression constructs. However, the conventional CRISPR-Cas9 system has several limitations. In this review, we describe the methods that were developed to overcome these limitations using yeast cells. We focus on three types of developments: reducing the frequency of unintended editing to both non-target and target sequences in the genome, inducing desired changes in the epigenetic state of the target region, and challenging the expansion of the CRISPR-Cas9 system to edit genomes within intracellular organelles such as mitochondria. These developments using yeast cells to overcome the limitations of the CRISPR-Cas9 system are a key factor driving the advancement of the field of genome editing.
Special Issue “Microbial Biodegradation and Biotransformation”
Authors: Ivshina Irina , Tyumina Elena
Published: April 2023

The current state of the environment is a major concern [...]
In Situ Mineralogical Constraints on Magmatic Process for Porphyry Deposits in the Upper Crust: A Case from Tongchang–Chang’anchong Porphyry Deposits, SW China
Authors: Wang Zixuan , Zheng Yuanchuan , Xu Bo , Shen Yang , Wang Lu
Published: April 2023

The magmatic process within upper crust encompasses various contents such as the transition between magmatic and hydrothermal systems and changes in oxygen fugacity (ƒO2), which ultimately play key roles in the formation of porphyry Cu deposits (PCDs). However, tracing these magmatic processes, especially in porphyry systems, is not an easy task. This study reported the detailed process of magmatic fluid exsolution and systematical variation of magmatic ƒO2 within the upper crust of a Tongchang–Chang’anchong porphyry Cu deposit, based on detailed investigations of mineral crystallization sequences and compositional features of the minerals in the fertile porphyries. Results indicate that the fertile porphyries show a high initial ƒO2, with ΔFMQ ≥ +3.0 (ΔFMQ is the deviation of logƒO2 from the fayalite–magnetite–quartz (FMQ) buffer). The magmatic ƒO2 (ΔFMQ) continued to decrease to ~+2 until fluid exsolution occurred at ~790 °C due to wall-rock contamination. The magmatic fluid exsolution process caused a temporary increase in the ƒO2 (to ΔFMQ = ~+3.4). The high magmatic ƒO2 during this process (790–750 °C) resulted in a higher content of ore-forming materials in the exsolved magmatic fluid. When the temperature dropped below 750 °C, the magmatic ƒO2 began to continuously decrease and eventually reached ΔFMQ = ~+0.6. The lower magmatic ƒO2 hindered the further migration of ore-forming materials through the exsolved fluid during this process (< 750 °C). Results of this study indicate that the initial magma during the upper crustal magmatic process of PCDs generally has a high ƒO2, and the contamination of reduced components can significantly decrease the magmatic ƒO2. The early magmatic fluid exsolution process can maintain a high magmatic ƒO2 condition, thereby efficiently extracting ore-forming minerals and producing ore-forming fluids, which is the key to the formation of PCDs. The latter continuous decrease in magmatic ƒO2 during the fluid exsolution process may be the reason preventing the Tongchang–Chang’anchong porphyry Cu deposit to form a giant PCD.
Petrogenesis and Tectonic Significance of Late Triassic A1-Type Granite from the West Section of North Qinling Orogenic Belt: Constraints from Geochronology and Geochemistry
Authors: Ji Shang , Li Zuochen , Pei Xianzhi , Pei Lei , Li Ruibao , Liu Chengjun , Chen Youxin , Lin Hao , Wang Mao
Published: April 2023

The North China Block and the South China Block collided in the Middle Triassic, but there is still a lack of consensus regarding the end of collisional orogeny and the closure time of the Paleo-Tethys. In this paper, we report zircon U–Pb ages and geochemistry for the Shimen pluton in the northern margin of the West Qinling Orogenic Belt to investigate its genesis and tectonic environment. The new findings allow to constrain the end time of the Triassic orogeny in the Qinling Orogenic Belt and the closure time of the Paleo-Tethys. The weighted average 206Pb/238U ages of the Shimen pluton are 218.6 ± 1.5 Ma and 221.0 ± 1.7 Ma. Thus, we suggest that the Shimen pluton crystallized at the 218.6 Ma and 221.0 Ma and was formed during the Late Triassic (Norian). The Shimen pluton is mainly syenogranite and has alkaline dark minerals aegirine–augite. It is composed of 73.45 to 77.80 wt.% SiO2, 8.28 to 9.76 wt.% alkali, and 11.35 to 13.58 wt.% Al2O3, with A/CNK ranging from 0.91 to 1.02 and 10,000 Ga/Al ranging from 2.39 to 3.15. These findings indicate that the Shimen pluton is typical A-type granite. The plutons have low rare earth element contents, ranging from 73.92 to 203.58 ppm, with a moderate negative Eu anomaly. All the samples are enriched in large-ion lithophile elements, such as Rb, Nd, Th and U, and light rare earth elements, and are depleted in high field strength elements, such as Nb, P, Zr, Ba, and Sr. The depletion of Ba, Sr, and Zr may be related to the fractionation and evolution of the granite. According to the petrological and geochemical characteristics, the Shimen pluton is an A1-type granite formed in an anorogenic extensional environment. Combined with its tectonic characteristics and petrogenesis, the Shimen pluton was probably formed by the partial melting of the crust under high temperature and low pressure in the intraplate environment after the subduction of the South China Block beneath the North China Block. This observation indicates that the Triassic orogeny in the Qinling Orogenic Belt had ended and the Paleo-Tethys-Mianlve Ocean had also closed by the Late Triassic (Norian).
Calcium Sulfate Crystallization in Presence of Fluorecent-Tagged Polyacrylate and Some Refinement of Scale Inhibition Mechanism
Authors: Trukhina Maria , Tkachenko Sergey , Ryabova Anastasia , Oshchepkov Maxim , Redchuk Anatoly , Popov Konstantin
Published: April 2023

Deposits of calcium sulfate scale on the surfaces of industrial equipment in distillation facilities, reverse osmosis desalination plants, in oil and gas industries lead to significant clogging of pipes and membranes and to a serious increase in production costs. For the mitigation of scale formation, the wide spectrum of antiscalants is applied. The present work is dedicated to the study of calcium sulfate deposition from supersaturated aqueous solutions in the presence of polyacrylic antiscalant with fluorescent marker (naphthalimide fragment) PAA-F1, which provides traceability of the scale inhibitor and a better understanding of its efficacy. A paradoxical phenomenon is being described here. Antiscalant causes a change in the crystal phase from bassanite to gypsum, significantly reduces the amount of deposit, but does not reveal the presence of its molecules either at the kink, step, or at selected edge sites of deposited crystals. Contrary to the predictions of the theory, it either stays in the aqueous phase, or forms its own separate phase Ca-PAA-F1. It is demonstrated that the antiscalant does not block the surfaces of calcium sulfate nuclei or crystal surfaces, but rather the foreign particles of nanodust with particle sizes around 1 nm, naturally occurring in any aqueous solution. As a result, the number of calcium nucleation sites is reduced, and the rate of scale formation decreases without any apparent antiscalant–scale interaction. On the grounds of the current experiment, the substoichiometric effect of scale inhibition obtained an alternative, quite stoichiometric explanation.
Proxy Archives Based on Marine Calcifying Organisms and the Role of Process-Based Biomineralization Concepts
Authors: Nehrke Gernot , Langer Gerald
Published: April 2023

Proxy archives based on marine calcifying organisms and their element and isotope signatures represent valuable tools in the reconstruction of past climates. Despite the fact that the underlying biomineralization processes behind the measured signatures are poorly understood, these tools work remarkably well. However, even though they work well many researchers felt the need to decipher the “black box” and understand the processes leading to the measured signatures. In this paper we assess how far we have come in understanding the biomineralization processes underpinning proxy signatures derived from marine calcifying organisms and how this understanding improved the way we use these proxy archives today. Biomineralization in the context of proxy research is an interdisciplinary field and cross-discipline communication can be challenging due to a lack of background in foreign disciplines. This often leads to misunderstanding and over- (or under-) estimation of certain concepts/methods/data. We, therefore, present a concise introduction to the topic, clarifying key concepts and their applicability to proxy interpretation.
Evaluating Software Tools for Lipid Identification from Ion Mobility Spectrometry–Mass Spectrometry Lipidomics Data
Authors: Ross Dylan H. , Guo Jian , Bilbao Aivett , Huan Tao , Smith Richard D. , Zheng Xueyun
Published: April 2023

The unambiguous identification of lipids is a critical component of lipidomics studies and greatly impacts the interpretation and significance of analyses as well as the ultimate biological understandings derived from measurements. The level of structural detail that is available for lipid identifications is largely determined by the analytical platform being used. Mass spectrometry (MS) coupled with liquid chromatography (LC) is the predominant combination of analytical techniques used for lipidomics studies, and these methods can provide fairly detailed lipid identification. More recently, ion mobility spectrometry (IMS) has begun to see greater adoption in lipidomics studies thanks to the additional dimension of separation that it provides and the added structural information that can support lipid identification. At present, relatively few software tools are available for IMS-MS lipidomics data analysis, which reflects the still limited adoption of IMS as well as the limited software support. This fact is even more pronounced for isomer identifications, such as the determination of double bond positions or integration with MS-based imaging. In this review, we survey the landscape of software tools that are available for the analysis of IMS-MS-based lipidomics data and we evaluate lipid identifications produced by these tools using open-access data sourced from the peer-reviewed lipidomics literature.
Microwave-Accelerated McKenna Synthesis of Phosphonic Acids: An Investigation
Authors: Mustafa Dana , Overhulse Justin M. , Kashemirov Boris A. , McKenna Charles E.
Published: April 2023

Phosphonic acids represent one of the most important categories of organophosphorus compounds, with myriad examples found in chemical biology, medicine, materials, and other domains. Phosphonic acids are rapidly and conveniently prepared from their simple dialkyl esters by silyldealkylation with bromotrimethylsilane (BTMS), followed by desilylation upon contact with water or methanol. Introduced originally by McKenna, the BTMS route to phosphonic acids has long been a favored method due to its convenience, high yields, very mild conditions, and chemoselectivity. We systematically investigated microwave irradiation as a means to accelerate the BTMS silyldealkylations (MW-BTMS) of a series of dialkyl methylphosphonates with respect to solvent polarity (ACN, dioxane, neat BTMS, DMF, and sulfolane), alkyl group (Me, Et, and iPr), electron-withdrawing P-substitution, and phosphonate–carboxylate triester chemoselectivity. Control reactions were performed using conventional heating. We also applied MW-BTMS to the preparation of three acyclic nucleoside phosphonates (ANPs, an important class of antiviral and anticancer drugs), which were reported to undergo partial nucleoside degradation under MW hydrolysis with HCl at 130–140 °C (MW-HCl, a proposed alternative to BTMS). In all cases, MW-BTMS dramatically accelerated quantitative silyldealkylation compared to BTMS with conventional heating and was highly chemoselective, confirming it to be an important enhancement of the conventional BTMS method with significant advantages over the MW-HCl method.
Description of Chemical Synthesis, Nuclear Magnetic Resonance Characterization and Biological Activity of Estrane-Based Inhibitors/Activators of Steroidogenesis
Authors: Poirier Donald
Published: April 2023

Steroid hormones play a crucial role in several aspects of human life, and steroidogenesis is the process by which hormones are produced from cholesterol using several enzymes that work in concert to obtain the appropriate levels of each hormone at the right time. Unfortunately, many diseases, such as cancer, endometriosis, and osteoporosis as examples, are caused by an increase in the production of certain hormones. For these diseases, the use of an inhibitor to block the activity of an enzyme and, in doing so, the production of a key hormone is a proven therapeutic strategy whose development continues. This account-type article focuses on seven inhibitors (compounds 1–7) and an activator (compound 8) of six enzymes involved in steroidogenesis, namely steroid sulfatase, aldo-keto reductase 1C3, types 1, 2, 3, and 12 of the 17β-hydroxysteroid dehydrogenases. For these steroid derivatives, three topics will be addressed: (1) Their chemical synthesis from the same starting material, estrone, (2) their structural characterization using nuclear magnetic resonance, and (3) their in vitro or in vivo biological activities. These bioactive molecules constitute potential therapeutic or mechanistic tools that could be used to better understand the role of certain hormones in steroidogenesis.
Variation in Phenolic Compounds, Antioxidant and Antibacterial Activities of Extracts from Different Plant Organs of Meadowsweet (Filipendula ulmaria (L.) Maxim.)
Authors: Savina Tamara , Lisun Valery , Feduraev Pavel , Skrypnik Liubov
Published: April 2023

Meadowsweet (Filipendula ulmaria (L.) Maxim.) has been widely used in the treatment of various diseases. The pharmacological properties of meadowsweet are derived from the presence of phenolic compounds of a diverse structure in sufficiently large quantities. The purpose of this study was to examine the vertical distribution of individual groups of phenolic compounds (total phenolics, flavonoids, hydroxycinnamic acids, catechins, proanthocyanidins, and tannins) and individual phenolic compounds in meadowsweet and to determine the antioxidant and antibacterial activity of extracts from various meadowsweet organs. It was found that the leaves, flowers, fruits, and roots of meadowsweet are characterized by a high total phenolics content (up to 65 mg g−1). A high content of flavonoids was determined in the upper leaves and flowers (117–167 mg g−1), with high contents of hydroxycinnamic acids in the upper leaves, flowers, and fruits (6.4–7.8 mg g–1); high contents of catechins and proanthocyanidins in the roots (45.1 and 3.4 mg g–1, respectively); and high tannin content in the fruits (38.3 mg g–1). Analysis of extracts by high-performance liquid chromatography (HPLC) showed that the qualitative and quantitative composition of individual phenolic compounds in various parts of the meadowsweet varied greatly. Among the flavonoids identified in meadowsweet, quercetin derivatives dominate, namely quercetin 3-O-rutinoside, quercetin 3-β-d-glucoside, and quercetin 4′-O-glucoside. Quercetin 4′-O-glucoside (spiraeoside) was found only in the flowers and fruits. Catechin was identified in the leaves and roots of meadowsweet. The distribution of phenolic acids across the plant was also uneven. In the upper leaves, a higher content of chlorogenic acid was determined, and in the lower leaves, a higher content of ellagic acid determined. In flowers and fruits, a higher contents of gallic, caftaric, ellagic, and salicylic acids were noted. Ellagic and salicylic acids were also dominant among phenolic acids in the roots. Based on the results of the analysis of antioxidant activity in terms of the ability to utilize the radicals of 2,2-diphenyl-1-picrylhydrazine (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazolino-6-sulfonic acid) (ABTS) and in terms of iron-reducing ability (FRAP), the upper leaves, flowers, and fruits of meadowsweet can be considered plant raw materials suitable to obtain extracts with high antioxidant activity. Extracts of plant fruits and flowers also showed high antibacterial activity against the bacteria Bacillus subtilis and Pseudomonas aeruginosa.
An Insight into the Potassium Currents of hERG and Their Simulation
Authors: Guidelli Rolando
Published: April 2023

By assuming that a stepwise outward movement of the four S4 segments of the hERG potassium channel determines a concomitant progressive increase in the flow of the permeant potassium ions, the inward and outward potassium currents can be simulated by using only one or two adjustable (i.e., free) parameters. This deterministic kinetic model differs from the stochastic models of hERG available in the literature, which usually require more than 10 free parameters. The K+ outward current of hERG contributes to the repolarization of the cardiac action potential. On the other hand, the K+ inward current increases with a positive shift in the transmembrane potential, in apparent contrast to both the electric and osmotic forces, which would concur in moving K+ ions outwards. This peculiar behavior can be explained by the appreciable constriction of the central pore midway along its length, with a radius < 1 Å and hydrophobic sacks surrounding it, as reported in an open conformation of the hERG potassium channel. This narrowing raises a barrier to the outward movement of K+ ions, inducing them to move increasingly inwards under a gradually more positive transmembrane potential.
Towards More Practical Methods for the Chemical Synthesis of Thioamides Using Sulfuration Agents: A Decade Update
Authors: Zhang Qiang , Soulère Laurent , Queneau Yves
Published: April 2023

Compounds possessing a thioamide function play a crucial role in organic synthesis, serving as key building blocks. They are also important in the pharmaceutical chemistry and drug design, owing to their ability to mimic the amide function in biomolecules while retaining or developing biological activity. From the synthetic viewpoint, several methods have been developed for preparing thioamides using sulfuration agents. The purpose of this review is to give an update of the last decade of contributions focusing on the formation of thioamides employing different sulfur sources. When appropriate, the cleanness and practicality of the new methods are highlighted.
Advances in the Field of Two-Dimensional Crystal-Based Photodetectors
Authors: Ye Xiaoling , Du Yining , Wang Mingyang , Liu Benqing , Liu Jiangwei , Jafri Syed Hassan Mujtaba , Liu Wencheng , Papadakis Raffaello , Zheng Xiaoxiao , Li Hu
Published: April 2023

Two-dimensional (2D) materials have sparked intense interest among the scientific community owing to their extraordinary mechanical, optical, electronic, and thermal properties. In particular, the outstanding electronic and optical properties of 2D materials make them show great application potential in high-performance photodetectors (PDs), which can be applied in many fields such as high-frequency communication, novel biomedical imaging, national security, and so on. Here, the recent research progress of PDs based on 2D materials including graphene, transition metal carbides, transition-metal dichalcogenides, black phosphorus, and hexagonal boron nitride is comprehensively and systematically reviewed. First, the primary detection mechanism of 2D material-based PDs is introduced. Second, the structure and optical properties of 2D materials, as well as their applications in PDs, are heavily discussed. Finally, the opportunities and challenges of 2D material-based PDs are summarized and prospected. This review will provide a reference for the further application of 2D crystal-based PDs.
A Visible-Light-Enhanced Heterogeneous Photo Degradation of Tetracycline by a Nano-LaFeO3 Catalyst with the Assistance of Persulfate
Authors: Hou Liwei , Wang Yanan , Zhou Fan , Liu Shuangyue , Fu Lin , Wang Lei , Zhang Changbo , Xue Weijie
Published: April 2023

Perovskites with nano-flexible texture structures and excellent catalytic properties have attracted considerable attention for persulfate activation in addressing the organic pollutants in water. In this study, highly crystalline nano-sized LaFeO3 was synthesized by a non-aqueous benzyl alcohol (BA) route. Under optimal conditions, an 83.9% tetracycline (TC) degradation and 54.3% mineralization were achieved at 120 min by using a coupled persulfate/photocatalytic process. Especially compared to LaFeO3-CA (synthesized by a citric acid complexation route), the pseudo-first-order reaction rate constant increased by 1.8 times. We attribute this good degradation performance to the highly specific surface area and small crystallite size of the obtained materials. In this study, we also investigated the effects of some key reaction parameters. Then, the catalyst stability and toxicity tests were also discussed. The surface sulfate radicals were identified as the major reactive species during the oxidation process. This study provided a new insight into nano-constructing a novel perovskite catalyst for the removal of tetracycline in water.
Anti-SARS-CoV-2 Immunoglobulins in Human Milk after Coronavirus Disease or Vaccination—Time Frame and Duration of Detection in Human Milk and Factors That Affect Their Titers: A Systematic Review
Authors: Dimitroglou Margarita , Sokou Rozeta , Iacovidou Nicoletta , Pouliakis Abraham , Kafalidis Georgios , Boutsikou Theodora , Iliodromiti Zoi
Published: April 2023

Human milk (HM) of mothers infected with or vaccinated against SARS-CoV-2 contains specific immunoglobulins, which may protect their offspring against infection or severe disease. The time frame and duration after infection or vaccination, during which these immunoglobulins are detected in HM, as well as the major factors that influence their levels, have not been fully elucidated. This systematic review aimed to collect the existing literature and describe the immune response, specifically regarding the immunoglobulins in HM after COVID-19 disease or vaccination in non-immune women. We conducted a systematic search of PubMed and Scopus databases to identify studies published up until 19 March 2023. In total, 975 articles were screened, and out of which 75 were identified as being relevant and were finally included in this review. Infection by SARS-CoV-2 virus primarily induces an IgA immune response in HM, while vaccination predominantly elevates IgG levels. These immunoglobulins give HM a neutralizing capacity against SARS-CoV-2, highlighting the importance of breastfeeding during the pandemic. The mode of immune acquisition (infection or vaccination) and immunoglobulin levels in maternal serum are factors that seem to influence immunoglobulin levels in HM. Further studies are required to determine the impact of other factors, such as infection severity, lactation period, parity, maternal age and BMI on immunoglobulin level in HM.
Very Low-Calorie Ketogenic Diet (VLCKD) as Pre-Operative First-Line Dietary Therapy in Patients with Obesity Who Are Candidates for Bariatric Surgery
Authors: Barrea Luigi , Verde Ludovica , Schiavo Luigi , Sarno Gerardo , Camajani Elisabetta , Iannelli Antonio , Caprio Massimiliano , Pilone Vincenzo , Colao Annamaria , Muscogiuri Giovanna
Published: April 2023

Bariatric surgery is currently the most effective method for achieving long-term weight loss and reducing the risk of comorbidities and mortality in individuals with severe obesity. The pre-operative diet is an important factor in determining patients’ suitability for surgery, as well as their post-operative outcomes and success in achieving weight loss. Therefore, the nutritional management of bariatric patients requires specialized expertise. Very low-calorie diets and intragastric balloon placement have already been studied and shown to be effective in promoting pre-operative weight loss. In addition, the very low-calorie ketogenic diet has a well-established role in the treatment of obesity and type 2 diabetes mellitus, but its potential role as a pre-operative dietary treatment prior to bariatric surgery has received less attention. Thus, this article will provide a brief overview of the current evidence on the very low-calorie ketogenic diet as a pre-operative dietary treatment in patients with obesity who are candidates for bariatric surgery.
Age and Sex Differences in the Association between Serum Vitamin E Levels and Depressive Symptoms: Korea National Health and Nutrition Examination Survey
Authors: Jeong Hyunwoo , Oh Jae Won , Son Nak-Hoon , Lee San
Published: April 2023

Studies have reported inconsistent results regarding the relationship between serum vitamin E levels and depressive symptoms. Furthermore, the potential modulating role of age and sex has been underexplored. We conduct an age- and sex-stratified investigation of the association between serological vitamin E status and depressive symptoms in a large nationwide sample. Data from the Korean National Health and Nutrition Examination Survey were analyzed (n = 4448). The participants were stratified into four groups according to age (<65 vs. ≥65 years) and sex. Each group was divided into tertiles of vitamin E/total lipid ratio, and Patient Health Questionnaire-9 (PHQ-9) scores were compared among the tertiles via multivariable linear regression analyses. The relationship between dietary supplement use and the proportions of tertiles was assessed in each group. With the middle tertile as the reference group, the low tertile of vitamin E/total lipid ratio was associated with increased PHQ-9 scores in younger females and older males after adjusting for all covariates, while the high tertile showed no significant associations with PHQ-9 scores in any group. The low tertile was associated with increased adjusted mean PHQ-9 scores by 0.53 and 1.02 compared to the middle tertile in younger females and older males, respectively. Dietary supplement use was related to higher vitamin E/total lipid ratio in all four groups. In conclusion, younger females and older males with a low vitamin E status showed worse depressive symptoms. These individuals may benefit from dietary interventions to prevent depressive symptoms.
Determinants of High Breastfeeding Self-Efficacy among Nursing Mothers in Najran, Saudi Arabia
Authors: Al-Thubaity DaifAllah D. , Alshahrani Mohammed A. , Elgzar Wafaa T. , Ibrahim Heba A.
Published: April 2023

Many factors have been found to correlate with satisfactory Exclusive Breastfeeding (EBF) practices. The relationships between EBF practices and associated factors are complex and multidimensional; Breastfeeding Self-Efficacy (BSE) is the most important psychological factor that may help the mother to overcome any expected barriers. This study investigates the determinants of high breastfeeding self-efficacy among Saudi nursing mothers. Methods: This is a descriptive cross-sectional study investigating the determinant of BSE among 1577 nursing mothers in primary health centers in Najran City, Saudi Arabia. The study uses a cluster random sampling technique. Data collection was performed from June 2022 to January 2023 using a self-reported questionnaire that encompasses the Breastfeeding Self-Efficacy Scale—Short Form (BSES-SF), Gender Friendly Breastfeeding Knowledge Scale (GFBKS), Iowa Infant Feeding Attitude Scale (IIFAS), and a basic data questionnaire to assess women’s demographic factors and obstetric history. Results: The mean score for all BSES-SF items was between 3.23–3.41, the highest mean score was in mothers who felt comfortable breastfeeding with family members present (3.41 ± 1.06), and the lowest mean was in mothers who could breastfeed their baby without using formula as a supplement (3.23 ± 0.94). The overall BSE score was high among 67% of the study participants. Binary logistic regression showed that being a housewife, being highly educated, having breastfeeding experience, and being multiparous are positive predictors for high BSE (p ≤ 0.001). In addition, having adequate breastfeeding knowledge and positive breastfeeding attitudes were positively associated with higher BSE (p = 0.000). Conclusion: BSE can be predicted by modifiable predictors such as mothers’ education, working status, parity, breastfeeding experience, adequate breastfeeding knowledge, and positive attitudes toward breastfeeding. If such predictors are considered during breastfeeding-related educational interventions, it could lead to more effective and sustainable effects in community awareness regarding breastfeeding.
Safety and Tolerability of Whole Soybean Products: A Dose-Escalating Clinical Trial in Older Adults with Obesity
Authors: Rebello Candida J. , Boué Stephen , Levy Ronald J. , Puyau Renée , Beyl Robbie A. , Greenway Frank L. , Heiman Mark L. , Keller Jeffrey N. , Reynolds Charles F. , Kirwan John P.
Published: April 2023

Soybean products have nutrients, dietary fiber, and phytoalexins beneficial for cardiovascular and overall health. Despite their high consumption in Asian populations, their safety in Western diets is debated. We conducted a dose-escalating clinical trial of the safety and tolerability of soybean products in eight older adults (70–85 years) with obesity. Whole green soybean pods grown under controlled conditions were processed to flour (WGS) at the United States Department of Agriculture using common cooking techniques such as slicing and heat treatment. WGS incorporated into food products was consumed at 10 g, 20 g, and 30 g/day for one week at each dose. The gastrointestinal outcomes, clinical biomarkers, and adverse events were evaluated. We explored the stimulation of phytoalexin (glyceollin) production in live viable soybean seeds (LSS-G). We compared the compositions of WGS and LSS-G with commercial soybean flour and its fermented and enzymatically hydrolyzed forms. We found that although 30 g WSG was well-tolerated, and it made participants feel full. Our processing produced glyceollins (267 µg/g) in LSS-G. Processing soybean flour decreased the iron content, but reduced the oligosaccharides, which could attenuate flatulence. Providing soybean flour at <30 g/day may be prudent for overall health and to prevent the exclusion of other food groups and nutrients in older adults with obesity.