bluuebunny/crossref_metadata_2025 huggingface dataset:
Personalized medicine: Function of CFTR variant p.Arg334Trp is rescued by currently available CFTR modulators
Authors: Railean Violeta , Rodrigues Cláudia S. , Ramalho Sofia S. , Silva Iris A. L. , Bartosch Jan , Farinha Carlos M. , Pankonien Ines , Amaral Margarida D.
Published: March 2023

Most of the 2,100 CFTR gene variants reported to date are still unknown in terms of their disease liability in Cystic Fibrosis (CF) and their molecular and cellular mechanism that leads to CFTR dysfunction. Since some rare variants may respond to currently approved modulators, characterizing their defect and response to these drugs is essential for effective treatment of people with CF (pwCF) not eligible for the current treatment. Here, we assessed how the rare variant, p.Arg334Trp, impacts on CFTR traffic and function and its response to existing CFTR modulators. To this end, we performed the forskolin-induced swelling (FIS) assay on intestinal organoids from 10 pwCF bearing the p.Arg334Trp variant in one or both alleles of the CFTR gene. In parallel, a novel p.Arg334Trp-CFTR expressing CFBE cell line was generated to characterize the variant individually. Results show that p.Arg334Trp-CFTR does not significantly affect the plasma membrane traffic of CFTR and evidences residual CFTR function. This CFTR variant is rescued by currently available CFTR modulators independently of the variant in the second allele. The study, predicting clinical benefit for CFTR modulators in pwCF with at least one p.Arg334Trp variant, demonstrates the high potential of personalized medicine through theranostics to extend the label of approved drugs for pwCF carrying rare CFTR variants. We recommend that this personalized approach should be considered for drug reimbursement policies by health insurance systems/national health services.
Reduction of drive for thinness and body dissatisfaction in people with self-reported dysregulated eating behaviors after intermittent theta burst stimulation (iTBS) of the left dorsolateral prefrontal cortex
Authors: Barone Jennifer , Oliveri Massimiliamo , Bonaventura Rosario Emanuele , Mangano Giuseppa Renata
Published: March 2023

Aim

This study aimed to explore the effect of intermittent theta burst stimulation (iTBS) of the right and left dorsolateral prefrontal cortex (DLPFC) in people with self-reported dysregulated eating behaviors but without a diagnosis of eating disorders (EDs).

Methods

Participants were randomly divided into two equivalent groups according to the side (right or left) of the hemisphere to be stimulated and they were tested before and after a single iTBS session. Outcome measurements were scores on self-report questionnaires assessing psychological dimensions related to eating behaviors (EDI-3), anxiety (STAI-Y), and tonic electrodermal activity.

Results

The iTBS interfered with both psychological and neurophysiological measures. Significant variations of physiological arousal after iTBS of both the right and left DLPFC were witnessed by increased mean amplitude of non-specific skin conductance responses. With regard to the psychological measures, the iTBS on the left DLPFC significantly reduced the scores of the EDI-3 subscales drive for thinness and body dissatisfaction. Interestingly, these two scales are two of the three EDI-3 clinic scales (drive for thinness, body dissatisfaction, and bulimia) used as specific markers to assess the onset and/or maintenance of eating disorders.

Conclusion

Our results show that the left DLPFC iTBS has an impact on the psychological dimensions that are risk factors for the onset of eating disorders, suggesting that an altered hemispheric asymmetry similar to that encountered in clinical populations is present in normal subjects even in the absence of clinical symptoms.
On developing a practical safety culture for the advanced reactor workforce: Experiences of working with sodium
Authors: LaBrier Daniel , Pope Chad , Marcum Wade
Published: March 2023

The Versatile Test Reactor is meant to fill a need in the advanced nuclear reactor development community. Accelerated nuclear testing of fuels and materials is needed to allow designs to proceed. To accomplish this, a fast neutron spectrum is needed. To have a fast neutron spectrum, the reactor coolant needs to not only have excellent heat transfer properties to sufficiently cool the reactor core, but also not significantly slow neutrons down following their birth in the fission process. Sodium is an excellent coolant choice that can fulfill these needs and many others. Sodium is the sixth most abundant element in the Earth’s crust and can be acquired easily through simple chemical separation methods. Sodium, however, reacts when in contact with water and air, but with proper precautions, these scenarios can be avoided. Nuclear reactors that utilize molten sodium as a primary heat transfer medium [a.k.a. sodium-cooled fast-neutron reactors, or SFRs, Figure 1 (1)] provide unique challenges in their daily operation, and therefore require focused training for researchers, scientists, staff, and employees alike to understand these unique challenges. Therefore, a comprehensive understanding of sodium properties is needed by all personnel involved in the design, construction, operation, experiment planning and execution of SFRs. Additionally, several commercial advanced reactor designs are under development which will use sodium for the reactor coolant. These advanced reactor designs include the Natrium reactor from TerraPower and GE-Hitachi (2), the ARC-100 reactor from Advanced Reactor Concepts (3), and the Aurora reactor from Oklo (4).
MIR4435-2HG in exosomes promotes gastric carcinogenesis by inducing M2 polarization in macrophages
Authors: Li Chaofeng , Chen Zhengju , Gao Jinli , Tang Tao , Zhou Lei , Zhang Guochao , Zhang Dongdong , Shen Chao , Guo Lei , Fu Tao
Published: March 2023

Gastric cancer (GC) is a cancer with a high mortality rate. lncRNAs play a role in regulating GC tumorigenesis. In this paper, we analyzed differentially expressed lncRNAs between GC and adjacent normal tissues using multiple bioinformatics tools to identify new potential targets in GC. Cell viability and migration ability were detected using the Cell Counting Kit-8 (CCK-8) and transwell assays, MIR4435-2HG was negatively correlated with the survival rate of GC patients, and by inhibiting the activity of MIR4435-2HG, the viability and migration ability of GC cells could be reduced. In addition, RT- qPCR and western blot to detect gene and protein level expression, transmission electron microscopy and nanoparticle tracking analysis (NTA) to study the efficiency of exosome isolation, and flow cytometry to observe cell differentiation were employed, delivery of MIR4435-2HG shRNA via MKN45 cell-derived exosomes significantly reversed the MKN45 exosome-induced M2 polarization in macrophages. Furthermore, the low expression of MIR4435-2HG in MKN45 cell-derived exosomes inhibited the Jagged1/Notch and JAK1/STAT3 pathways in macrophages; MIR4435-2HG downregulated exosomes were found to significantly inhibit GC tumor growth in vivo by establishing a mouse model. In short, MKN45 cell-derived exosomes deliver lncRNA MIR4435-2HG, which promotes gastric carcinogenesis by inducing macrophage M2 polarization.
Metabolic reprogramming by miRNAs in the tumor microenvironment: Focused on immunometabolism
Authors: Alshahrani Shadia Hamoud , Ibrahim Yousif Saleh , Jalil Abduladheem Turki , Altoum Abdelgadir Alamin , Achmad Harun , Zabibah Rahman S. , Gabr Gamal A. , Ramírez-Coronel Andrés Alexis , Alameri Ameer A. , Qasim Qutaiba A. , Karampoor Sajad , Mirzaei Rasoul
Published: March 2023

MicroRNAs (miRNAs) are emerging as a significant modulator of immunity, and their abnormal expression/activity has been linked to numerous human disorders, such as cancer. It is now known that miRNAs potentially modulate the production of several metabolic processes in tumor-associated immune cells and indirectly via different metabolic enzymes that affect tumor-associated signaling cascades. For instance, Let-7 has been identified as a crucial modulator for the long-lasting survival of CD8+ T cells (naive phenotypes) in cancer by altering their metabolism. Furthermore, in T cells, it has been found that enhancer of zeste homolog 2 (EZH2) expression is controlled via glycolytic metabolism through miRNAs in patients with ovarian cancer. On the other hand, immunometabolism has shown us that cellular metabolic reactions and processes not only generate ATP and biosynthetic intermediates but also modulate the immune system and inflammatory processes. Based on recent studies, new and encouraging approaches to cancer involving the modification of miRNAs in immune cell metabolism are currently being investigated, providing insight into promising targets for therapeutic strategies based on the pivotal role of immunometabolism in cancer. Throughout this overview, we explore and describe the significance of miRNAs in cancer and immune cell metabolism.
Hypofractionated whole breast irradiation with simultaneous integrated boost in breast cancer using helical tomotherapy with or without regional nodal irradiation: A report of acute toxicities
Authors: Chitapanarux Imjai , Nobnop Wannapha , Onchan Wimrak , Klunklin Pitchayaponne , Nanna Thongtra , Sitathanee Chomporn , Kulpisitthicharoen Sutthisak , Sripan Patumrat
Published: March 2023

Purpose

We prospectively investigated the acute toxicities focusing on skin and hematologic function in breast cancer patients who received hypofractionated whole breast irradiation with simultaneous integrated boost (HF-WBI-SIB) with helical tomotherapy (HT), with or without regional nodal irradiation (RNI).

Methods

The dose of WBI and RNI was 42.4 Gy in 16 fractions. Tumor bed was prescribed to 49.6 Gy in 16 fractions simultaneously. The association between the worst grade of acute toxicities during treatment and receiving RNI was analyzed. The integral dose to the whole body between the two groups was also compared.

Results

Between May 2021 and May 2022, 85 patients were enrolled; 61 patients received HF-WBI-SIB only (71.8%) and 24 patients (28.2%) received HF-WBI-SIB with RNI. Grade 2 acute skin toxicity was found in 1.2%. The most frequent grade 2 or more hematologic toxicity was leukopenia, which occurred in 4.8% and 11% in the 2nd and 3rd week, respectively. Mean whole body integral dose was significantly higher in patients treated with RNI compared to patients treated without RNI: 162.8 ± 32.8 vs. 120.3 ± 34.7 Gy-L (p-value < 0.001). There was no statistically significant difference in acute grade 2 or more skin and hematologic toxicities between the two groups.

Conclusions

HF-WBI-SIB with or without RNI is feasible with acceptable acute skin and hematologic toxicities. RNI and whole body integral dose were not associated with these acute toxicities.
Exosomes and osteosarcoma drug resistance
Authors: Fu Huichao , Wu Yunjiao , Chen Jianbai , Hu Xing , Wang Xiaoyan , Xu Gongping
Published: March 2023

Osteosarcoma (OS) is a primary malignant tumor of bone characterized by the formation of bone tissue or immature bone by tumor cells. Because of its multi-drug resistance, even with the improvement of chemotherapy and the use of targeted drugs, the survival rate of osteosarcoma (OS) is still less than 60%, and it is easy to metastasize, which is a difficulty for many clinicians and researchers. In recent years, with the continuous research on exosomes, it has been found that exosomes play a role in the diagnosis, treatment and chemotherapy resistance of osteosarcoma due to their unique properties. Exosomes can reduce the intracellular accumulation of chemotherapeutic drugs by mediating drug efflux, thus inducing chemotherapeutic resistance in OS cells. Exosomal goods (including miRNA and functional proteins) carried by exosomes also show great potential in affecting the drug resistance of OS. In addition, miRNA carried by exosomes and exosomes exist widely in tumor cells and can reflect the characteristics of parent cells, so it can also be used as a biomarker of OS. At the same time, the development of nanomedicine has given a new hope for the treatment of OS. Exosomes are regarded as good natural nano-carriers by researchers because of their excellent targeted transport capacity and low toxicity, which will play an important role in the field of OS therapy in the future. This paper reviews the internal relationship between exosomes and OS chemotherapy resistance, discusses the broad prospects of exosomes in the field of diagnosis and treatment of OS, and puts forward some suggestions for the study of the mechanism of OS chemotherapy resistance.
A comprehensive overview of exosome lncRNAs: Emerging biomarkers and potential therapeutics in gynecological cancers
Authors: Wang Min , Fu Lulu , Xu Ying , Ma Shuai , Zhang Xueying , Zheng Lianwen
Published: March 2023

Ovarian, endometrial, and cervical cancer are common gynecologic malignancies, and their incidence is increasing year after year, with a younger patient population at risk. An exosome is a tiny “teacup-like” blister that can be secreted by most cells, is highly concentrated and easily enriched in body fluids, and contains a large number of lncRNAs carrying some biological and genetic information that can be stable for a long time and is not affected by ribonuclease catalytic activity. As a cell communication tool, exosome lncRNA has the advantages of high efficiency and high targeting. Changes in serum exosome lncRNA expression in cancer patients can accurately reflect the malignant biological behavior of cancer cells. Exosome lncRNA has been shown in studies to have broad application prospects in cancer diagnosis, monitoring cancer recurrence or progression, cancer treatment, and prognosis. The purpose of this paper is to provide a reference for clinical research on the pathogenesis, diagnosis, and treatment of gynecologic malignant tumors by reviewing the role of exosome lncRNA in gynecologic cancers and related molecular mechanisms.
Endovascular image-guided sampling of tumor-draining veins provides an enriched source of oncological biomarkers
Authors: Tamrazi Anobel , Sundaresan Srividya , Gulati Aishwarya , Tan Frederick J. , Wadhwa Vibhor , Bartlett Bjarne R. , Diaz Luis A. Jr.
Published: March 2023

Introduction

Circulating tumor-derived biomarkers can potentially impact cancer management throughout the continuum of care. This small exploratory study aimed to assess the relative levels of such biomarkers in the tumor-draining vascular beds in patients with solid tumors compared to levels in their peripheral veins.

Methods

Using an endovascular image-guided approach, we obtained blood samples from peripheral veins and other vascular compartments–including the most proximal venous drainage from solid tumors–from a set of nine oncology patients with various primary and metastatic malignancies. We then interrogated these samples for a panel of oncological biomarkers, including circulating tumor cells (CTCs), exosome-derived microRNAs (miRNAs), circulating tumor DNA (ctDNA) mutations, and certain cancer-related proteins/biochemical markers.

Results

We found substantially higher levels of CTCs, certain miRNAs, and specific ctDNA mutations in samples from vascular beds closer to the tumor compared with those from peripheral veins and also noted that some of these signals were altered by treatment procedures.

Discussion

Our results indicate that tumor-proximal venous samples are highly enriched for some oncological biomarkers and may allow for more robust molecular analysis than peripheral vein samples.
Identification of cuproptosis-based molecular subtypes, construction of prognostic signature and characterization of immune landscape in colon cancer
Authors: Wang Xu , Zuo Xiaomin , Hu Xianyu , Liu Yuyao , Wang Zhenglin , Chan Shixin , Sun Rui , Han Qijun , Yu Zhen , Wang Ming , Zhang Huabing , Chen Wei
Published: March 2023

Background

Cuproptosis is a newly discovered form of cell death induced by targeting lipoacylated proteins involved in the tricarboxylic acid cycle. However, the roles of cuproptosis-related genes (CRGs) in the clinical outcomes and immune landscape of colon cancer remain unknown.

Methods

We performed bioinformatics analysis of the expression data of 13 CRGs identified from a previous study and clinical information of patients with colon cancer obtained from The Cancer Genome Atlas and Gene Expression Omnibus databases. Colon cancer cases were divided into two CRG clusters and prognosis-related differentially expressed genes. Patient data were separated into three corresponding distinct gene clusters, and the relationships between the risk score, patient prognosis, and immune landscape were analyzed. The identified molecular subtypes correlated with patient survival, immune cells, and immune functions. A prognostic signature based on five genes was identified, and the patients were divided into high- and low-risk groups based on the calculated risk score. A nomogram model for predicting patient survival was developed based on the risk score and other clinical features.

Results

The high-risk group showed a worse prognosis, and the risk score was related to immune cell abundance, microsatellite instability, cancer stem cell index, checkpoint expression, immune escape, and response to chemotherapeutic drugs and immunotherapy. Findings related to the risk score were validated in the imvigor210 cohort of patients with metastatic urothelial cancer treated with anti-programmed cell death ligand 1.

Conclusion

We demonstrated the potential of cuproptosis-based molecular subtypes and prognostic signatures for predicting patient survival and the tumor microenvironment in colon cancer. Our findings may improve the understanding of the role of cuproptosis in colon cancer and lead to the development of more effective treatment strategies.
Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis
Authors: Arenbaoligao  , Guo Xinrui , Xiong Jiahao , Zhang Shuangshuang , Yang Yuewen , Chen Dapeng , Xie Yu
Published: March 2023

Inhibition of epithelial ferroptosis in colonic tissues relieved clinical symptoms and improved endoscopic presentations in inflammatory bowel disease (IBD). Kumatakenin, the main ingredient of traditional Chinese medicinal cloves and Alpinia purpurata, is reported to possess therapeutic benefits. However, whether kumatakenin could inhibit ferroptosis and further alleviate colitis remains unclear. Here, we measured the effects of kumatakenin on ferroptosis of colonic epithelial cells from colitis mice. The colitis model was induced in mice by oral intake of 2.5% dextran sulfate sodium in drinking water. RNA sequencing was performed to investigate the mechanism underlying kumatakenin-mediated effects on colitis. The results showed that different doses of kumatakenin significantly alleviated symptoms and suppressed intestinal inflammation in the colitis mouse model. Kumatakenin supplementation decreased cellular iron levels and suppressed ferroptosis in epithelial cells from colitis mice. RNA sequencing, qPCR, and pharmacological inhibition assays showed that kumatakenin reduced cellular iron levels and suppressed ferroptosis in epithelial cells from colitis mice at least partially by upregulating expression of enolase (Eno-3). Furthermore, kumatakenin decreased iron levels in epithelial cells by modulating the Eno3-iron regulatory protein (IRP1) axis. Molecular docking results revealed that kumatakenin could bind Eno3 via hydrogen bonding with the amino acid residues Thr208, Val206, and Pro203. This work will provide a scientific basis for the clinical use of kumatakenin in the treatment of colitis.
Medical ozone arrests oxidative damage progression and regulates vasoactive mediator levels in elderly patients (60–70 years) with oxidative etiology diseases
Authors: León Fernández Olga Sonia , Oru Gabriel Takon , Viebahn-Hänsler Renate , López Cabreja Gilberto , Serrano Espinosa Irainis , García Fernández Elizabeth
Published: March 2023

Medical ozone reestablishes cellular redox balance so that it may be a valid therapeutic approach in the prevention and management of age-related diseases with oxidative etiology in older people. The aim of this study is to evaluate oxidative stress and some vasoactive substances in elderly (60–70 years) rheumatoid arthritis patients with diabetes and hypertension, as well as another group with bronchial asthma patients in order to demonstrate the beneficial effects of medical ozone in the prevention and therapy of age-related diseases in these age groups. A randomized clinical study with 45 older patients (60–70 years) was performed. Group I (n = 15) with rheumatoid arthritis + diabetes and hypertension received no ozone treatment, and group II (n = 30) was treated with medical ozone. This group was divided into two subgroups (n = 15 each), group IIa: the same as group I + medical ozone and group IIb: bronchial asthma patients. Indicators of RA in I and IIa groups were evaluated. Redox balance was assessed through defense and injury biomarkers. Thromboxane A2 (TXA2) and prostacyclin levels were assessed in group IIb patients. Medical ozone arrested oxidative injury progression in the Ia group and decreased thromboxane levels and the TXA2/6-keto PGF1α ratio in the IIb group. Medical ozone arrested the progression of oxidative damage and modulated those endogenous mechanisms that promote a suitable redox status and TXA2/PGI2 balance. These results suggest that medical ozone may become a standard approach in the prevention and management of age-related oxidative diseases in elderly people.
Chronic treatment with glucocorticoids does not affect egg quality but increases cortisol deposition into egg albumen and elicits changes to the heterophil to lymphocyte ratio in a sex-dependent manner
Authors: Oluwagbenga E. M. , Tetel V. , Tonissen S. , Karcher D. M. , Fraley G. S.
Published: March 2023

During chronic stress, there is an initial increase in glucocorticoid (GC) levels, but they then return to low, albeit not baseline, levels. Recent studies have renewed interest in cortisol in that it may also have important roles in the stress response. The purpose of our study was to test the hypothesis that chronic treatment with low levels of either corticosterone or cortisol would alter HLR and immune organ morphometrics. Further, we wanted to determine if chronic treatment with either GC would elicit an increase in cortisol levels in egg albumen. To test our hypotheses, we implanted silastic capsules that contained corticosterone, cortisol, or empty capsules as controls (N = 5/sex/treatment). Blood serum, smears, body weights, and egg quality data were collected. Ducks were then euthanized and body weight, weights of spleens, livers, and the number of active follicles were recorded. Albumen GC levels were assessed using mass spectrometry. Data were analyzed using a 2- or 3-way ANOVA as appropriate and post-hoc with Fishers PLSD. No treatment elicited differences in egg quality measures or body weight compared to controls. Corticosterone treatment did elicit an increase in serum corticosterone (p < 0.05), but not cortisol, levels compared to controls in both sexes. Both cortisol and corticosterone treatments increased (p < 0.05) serum levels of cortisol compared to controls. Relative spleen weights were higher (p < 0.05) in hens following corticosterone but not cortisol treatment. No other organs showed any differences among the treatment groups. Both GCs elicited an increase (p < 0.001) in HLR in hens at all time-points over the 2-week treatment period compared to controls. Cortisol, not corticosterone, only elicited an increase in HLR for drakes (p < 0.05) compared to controls but only at day 1 after implants. Chronic treatment with cortisol, but not corticosterone, elicited an increase (p < 0.01) in egg albumen cortisol levels compared to other groups. Corticosterone was not detected in any albumen samples. Our results suggest that glucocorticoids elicit differential effects and although corticosterone has been stated to be the predominant GC in avian species, cortisol may provide critical information to further understand bird welfare.
Serendipita indica promotes P acquisition and growth in tea seedlings under P deficit conditions by increasing cytokinins and indoleacetic acid and phosphate transporter gene expression
Authors: Rong Zi-Yi , Lei An-Qi , Wu Qiang-Sheng , Srivastava Anoop Kumar , Hashem Abeer , Abd_Allah Elsayed Fathi , Kuča Kamil , Yang Tianyuan
Published: March 2023

The culturable endophytic fungus Serendipita indica has many beneficial effects on plants, but whether and how it affects physiological activities and phosphorus (P) acquisition of tea seedlings at low P levels is unclear. The objective of this study was to analyze the effects of inoculation with S. indica on growth, gas exchange, chlorophyll fluorescence, auxins, cytokinins, P levels, and expressions of two phosphate transporter (PT) genes in leaves of tea (Camellia sinensis L. cv. Fudingdabaicha) seedlings grown at 0.5 μM (P0.5) and 50 μM (P50) P levels. Sixteen weeks after the inoculation, S. indica colonized roots of tea seedlings, with root fungal colonization rates reaching 62.18% and 81.34% at P0.5 and P50 levels, respectively. Although plant growth behavior, leaf gas exchange, chlorophyll values, nitrogen balance index, and chlorophyll fluorescence parameters of tea seedlings were suppressed at P0.5 versus P50 levels, inoculation of S. indica mitigated the negative effects to some extent, along with more prominent promotion at P0.5 levels. S. indica inoculation significantly increased leaf P and indoleacetic acid concentrations at P0.5 and P50 levels and leaf isopentenyladenine, dihydrozeatin, and transzeatin concentrations at P0.5 levels, coupled with the reduction of indolebutyric acid at P50 levels. Inoculation of S. indica up-regulated the relative expression of leaf CsPT1 at P0.5 and P50 levels and CsPT4 at P0.5 levels. It is concluded that S. indica promoted P acquisition and growth in tea seedlings under P deficit conditions by increasing cytokinins and indoleacetic acid and CsPT1 and CsPT4 expression.
The effect of level-marked mathematics tasks on students’ self-efficacy: An experimental study
Authors: Herset Maria , El Ghami Mohamed , Bjerke Annette Hessen
Published: March 2023

This study investigates whether and to what extent students’ self-efficacy in mathematics is affected by level-marked mathematics tasks. An online survey with an experimental design was used to collect data from lower secondary school students in Norway (n = 436). The effect of level-marked mathematics tasks was measured by comparing students’ responses to tasks with no level marking with their responses to the same tasks marked as being easy, medium or difficult. The study’s design was set up carefully, featuring experimental and control groups. A Wilcoxon test showed a significant gap in students’ self-efficacy when approaching the same tasks without level marking and with difficult-level marking. In addition, a Friedman test showed that the gap between students’ self-efficacy when encountering the same task with and without level marking expanded significantly with increasing difficulty markings. This result has implications for students in terms of their mathematics learning and for mathematics teachers in terms of their future differentiation initiatives.
Reward prospect affects strategic adjustments in stop signal task
Authors: Giuffrida Valentina , Marc Isabel Beatrice , Ramawat Surabhi , Fontana Roberto , Fiori Lorenzo , Bardella Giampiero , Fagioli Sabrina , Ferraina Stefano , Brunamonti Emiliano , Pani Pierpaolo
Published: March 2023

Interaction with the environment requires us to predict the potential reward that will follow our choices. Rewards could change depending on the context and our behavior adapts accordingly. Previous studies have shown that, depending on reward regimes, actions can be facilitated (i.e., increasing the reward for response) or interfered (i.e., increasing the reward for suppression). Here we studied how the change in reward perspective can influence subjects’ adaptation strategy. Students were asked to perform a modified version of the Stop-Signal task. Specifically, at the beginning of each trial, a Cue Signal informed subjects of the value of the reward they would receive; in one condition, Go Trials were rewarded more than Stop Trials, in another, Stop Trials were rewarded more than Go Trials, and in the last, both trials were rewarded equally. Subjects participated in a virtual competition, and the reward consisted of points to be earned to climb the leaderboard and win (as in a video game contest). The sum of points earned was updated with each trial. After a learning phase in which the three conditions were presented separately, each subject performed 600 trials testing phase in which the three conditions were randomly mixed. Based on the previous studies, we hypothesized that subjects could employ different strategies to perform the task, including modulating inhibition efficiency, adjusting response speed, or employing a constant behavior across contexts. We found that to perform the task, subjects preferentially employed a strategy-related speed of response adjustment, while the duration of the inhibition process did not change significantly across the conditions. The investigation of strategic motor adjustments to reward’s prospect is relevant not only to understanding how action control is typically regulated, but also to work on various groups of patients who exhibit cognitive control deficits, suggesting that the ability to inhibit can be modulated by employing reward prospects as motivational factors.
Longing for normalcy in couple relationships: How chronic illness and care dependency change the relationship of long-married couples
Authors: Niedling Katharina , Hämel Kerstin
Published: March 2023

Introduction

Coping with chronic illness and care dependency in a marital dyad challenges many older couples. In our qualitative research study, we are interested in how long-married spouses in Germany experience their couple relationship while dealing with long-term care and adapting everyday life to the care situation.

Methods

We conducted problem-centered interviews with 17 spouses according to the interpretive-reconstructive documentary method.

Results

We derived four thematic areas: (1) partner(ship) disappears behind the disease; (2) partners struggle with changing tasks and roles; (3) caring partners mourn the loss of intimacy; and (4) partners strive to rebalance the partnership.

Discussion

When chronic illness and care dependency enter couples' lives, the self-image as husband or wife is affected. Primary health care professionals should be sensitive to the specific constellation of care in couple relationships and recognize the significance of this dyadic relationship as living in a satisfying couple relationship is essential for the health and wellbeing of both partners.
Effect of concurrent training on physical performance and quality of life in children with malignancy: A systematic review and meta-analysis
Authors: Zang Wanli , Fang Mingqing , Chen Haohao , Huang Xinmeng , Li Dong , Yan Jin , Shu Heng , Zhao Mingyuan
Published: March 2023

Objective

This study aims to evaluate the intervention effect of concurrent training on children with malignant tumors to provide evidence for prescribing exercise for children with malignant tumors.

Methods

Twelve databases were searched from inception to October 15, 2022. Two researchers independently screened the literature, evaluated the quality, extracted the data, and performed the meta-analysis using R.

Result

A total of nine randomized controlled trials involving 371 children were included in this study. The meta-analysis revealed that muscle strength was significantly greater in the exercise group compared to the usual care group [SMD = 0.26, 95% CI (0.04, 0.48), P = 0.023], with subgroup analysis showing no significant difference in upper limb [SMD = 0.13, 95% CI (−0.17, 0.43), P = 0.318] and a considerable difference in lower limb strength [SMD = 0.41, 95% CI (0.08, 0.74), P = 0.015]. Physical activity [SMD = 0.57, 95% CI (0.03, 1.1), P = 0.038], timed up and down stairs test [SMD = −1.22, 95% CI (−2.04, −0.4), P = 0.004], 6-min walking ability [SMD = 0.75, 95% CI (0.38, 1.11), P < 0.01], quality of life [SMD = 0.28, 95% CI (0.02, 0.53), P = 0.033], and cancer-related fatigue [SMD = −0.53, 95% CI (−0.86, −0.19), P = 0.002] were significantly better than the usual care group. There were no significant differences in peak oxygen uptake [SMD = 0.13, 95% CI (−0.18, 0.44), P = 0.397], depression [SMD = 0.06, 95% CI (−0.38, 0.5), P = 0.791], and withdrawal rates [RR = 0.59, 95% CI (0.21, 1.63), P = 0.308] between the two groups.

Conclusion

Concurrent training could improve physical performance for children with malignancy but had no significant effect on mental health. Because the quality level of evidence is mostly very low, future high-quality randomized controlled trials are required to confirm these findings.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=364140, identifier CRD42022308176.
Expired patents: An opportunity for higher education institutions
Authors: Mafu Mhlambululi
Published: March 2023

Patent protection typically lasts about 20 years from the filing date and is in exchange for sufficiently disclosing the invention. The disclosure aims to enrich technical knowledge globally, promote creativity and technological innovation and contribute to sustainable socio-economic development. After this protection period, the patent expires, and in principle, any person may begin practicing the specific subject matter previously protected by the patent. Since the invention originally met all the patentability requirements, it was disclosed sufficiently to stimulate further innovation by others through a thorough understanding of existing developments in the patent literature. Thus, in addition to scholarly research articles, this makes patents potentially valuable sources of technical information in research and academia, unlocking new technology opportunities. We use the exploratory research method to study a potentially genuine and vital research stream that uncovers the overlooked yet valuable scientific and technical information sources that higher education institutions could utilize to complement academic research articles. This work establishes a necessary research agenda that critically challenges researchers to tap into the immediately available and promising technology opportunities presented by patents in the public domain. Using case studies to gain in-depth, multi-faceted explorations about the impact of these patents, we find that technologies contained in expired patents, abandoned patents, and technologies not protected by IPRs, resulting in improved research quality and increased collaboration with industry, if adequately exploited and integrated with other technologies. Moreover, this could lead to increased academic patenting and commercialization with support from the university's Technology Transfer Office.
Progress in islet xenotransplantation: Immunologic barriers, advances in gene editing, and tolerance induction strategies for xenogeneic islets in pig-to-primate transplantation
Authors: Eisenson Daniel L. , Hisadome Yu , Santillan Michelle R. , Yamada Kazuhiko
Published: March 2023

Islet transplantation has emerged as a curative therapy for diabetes in select patients but remains rare due to shortage of suitable donor pancreases. Islet transplantation using porcine islets has long been proposed as a solution to this organ shortage. There have already been several small clinical trials using porcine islets in humans, but results have been mixed and further trials limited by calls for more rigorous pre-clinical data. Recent progress in heart and kidney xenograft transplant, including three studies of pig-to-human xenograft transplant, have recaptured popular imagination and renewed interest in clinical islet xenotransplantation. This review outlines immunologic barriers to islet transplantation, summarizes current strategies to overcome these barriers with a particular focus on approaches to induce tolerance, and describes an innovative strategy for treatment of diabetic nephropathy with composite islet-kidney transplantation.
Comparative analysis of postoperative curative effect of liver wedge resection and liver IVb + V segment resection in patients with T2b gallbladder cancer
Authors: Wang Zichao , Liu Hongliang , Huang Yong , Wang Jun , Li Junjie , Liu Lingpeng , Huang Mingwen
Published: March 2023

Objective

To compare the therapeutic effects of wedge resection and liver segment IVb + V resection on patients with T2b gallbladder cancer.

Methods

The clinical and pathological data of 40 patients with gallbladder cancer admitted to the Second Affiliated Hospital of Nanchang University from January 2017 to November 2019 were retrospectively analyzed, and they were divided into two groups according to different surgical methods. The control group underwent liver wedge resection, while the experimental group underwent liver segment IVb + V resection. Preoperative age, bilirubin index, tumor markers, postoperative complications and survival were compared between the two groups. Log-rank test was used for univariate analysis, and Cox proportional hazards regression model was used for multivariate analysis. Kaplan-Meier survival curves were drawn.

Results

Univariate analysis showed that tumor markers and degree of differentiation were the risk factors for the prognosis of patients with gallbladder carcinoma after radical cholecystectomy (P < 0.05). Multivariate analysis showed that the elevation of CA125 and CA199, poor differentiation, and Lymph node metastasis were independent risk factors for the prognosis of gallbladder carcinoma after radical resection (P < 0.05). According to the 3-year survival rate, the survival rate of patients with liver 4B + 5 segment resection + cholecystectomy was higher than that of patients with 2 cm liver wedge resection + cholecystectomy (41.6% vs. 72.7%).

Conclusion

Patients with T2b gallbladder cancer should be treated with liver segment IVb + V resection, which is helpful to improve the prognosis of patients and should be widely promoted.
Tandem mass tag-based quantitative proteomics analyses of a chicken-original virulent and its attenuated Histomonas meleagridis strain in China
Authors: Chen Qiao-Guang , Zhang Yu-Ming , Chen Chen , Wang Shuang , Li Zai-Fan , Hou Zhao-Feng , Liu Dan-Dan , Tao Jian-Ping , Xu Jin-Jun
Published: March 2023

Introduction

Histomonas meleagridis can cause histomonosis in poultry. Due to the prohibition of effective drugs, the prevention and treatment of the disease requires new strategies. Questions about its pathogenic mechanisms and virulence factors remain puzzling.

Methods

To address these issues, a tandem mass tag (TMT) comparative proteomic analysis of a virulent strain and its attenuated strain of Chinese chicken-origin was performed.

Results

A total of 3,494 proteins were identified in the experiment, of which 745 proteins were differentially expressed (fold change ≥1.2 or ≤0.83 and p < 0.05), with 192 up-regulated proteins and 553 down-regulated proteins in the virulent strain relative to the attenuated strain.

Discussion

Surface protein BspA like, digestive cysteine proteinase, actin, and GH family 25 lysozyme were noted among the proteins up regulated in virulent strains, and these several proteins may be directly related to the pathogenic capacity of the histomonad. Ferredoxin, 60S ribosomal protein L6, 40S ribosomal protein S3, and NADP-dependent malic enzyme which associated with biosynthesis and metabolism were also noted, which have the potential to be new drug targets. The up-regulation of alpha-amylase, ras-like protein 1, ras-like protein 2, and involucrin in attenuated strains helps to understand how it is adapted to the long-term in vitro culture environment. The above results provide some candidate protein-coding genes for further functional verification, which will help to understand the molecular mechanism of pathogenicity and attenuation of H. meleagridis more comprehensively.
NAD+ biosynthesis metabolism predicts prognosis and indicates immune microenvironment for breast cancer
Authors: Yang Yuting , Wang Ze , He Mengqi , Diao Lihong , Yu Biyue , Li Dong
Published: March 2023

The growing evidence implies that tumor cells need to increase NAD+ levels by upregulating NAD+ biosynthesis to satisfy their growth demand. NAD+ biosynthesis metabolism is implicated in tumor progression. Breast cancer (BC) is the most common malignant malignancy in the world. Nevertheless, the prognostic significance of NAD+ biosynthesis and its relationship with the tumor immune microenvironment in breast cancer still need further investigation. In this study, we obtained the mRNA expression data and clinical information of BC samples from public databases and calculated the level of NAD+ biosynthesis activity by single-sample gene set enrichment analysis (ssGSEA). We then explored the relationship between the NAD+ biosynthesis score, infiltrating immune cells, prognosis significance, immunogenicity and immune checkpoint molecules. The results demonstrated that patients with high NAD+ biosynthetic score displayed poor prognosis, high immune infiltration, high immunogenicity, elevated PD-L1 expression, and might more benefit from immunotherapy. Taken together, our studies not only deepened the understanding of NAD+ biosynthesis metabolism of breast cancer but also provided new insights into personalized treatment strategies and immunological therapies to improve the outcomes of breast cancer patients.
Emotional Expectancies and Hostile Attributions as Predictors of Adolescents’ Expressions of Emotion with Parents
Authors: Lindsey Eric W.
Published: March 2023

The present study examined associations between adolescents’ Emotional Expectancies (EE), Hostile Attributions of Intent (HAI), and emotions expressed during interactions with their mother and father. Data were collected from 96 14- to 16-year-olds (27 African Americans, 38 European Americans, and 31 Latinos; a total of 51 girls and 45 boys) and their parents over a period of 2 years. Questionnaires completed by adolescents were used to assess emotional expectancies and hostile attributions of parents’ behavior. In both year one and year two, adolescent emotional expressiveness with parents was observed in a laboratory interaction session. Data revealed that both adolescents’ EE and HAI in reaction to ambiguous situations predicted their expression of positive emotion with their mother and father 1 year later. EE of happiness were positively related and EE of anger were negatively related to the expression of positive emotion with their mother and father. HAI were negatively related to the expression of positive emotion. Only HAI were related to a higher expression of anger with their mother and father. The findings indicate that HAI and EE represent distinct cognitive-emotional processes that contribute to individual differences in adolescents’ expressions of emotion with parents.
Laser-Induced Ignition and Combustion of Single Micron-Sized Al-Li Alloy Particles in High Pressure Air/N2
Authors: Xu Dunhui , Wang Fang , Li Shengji , Huang Xuefeng , Li Heping , Guo Yanhui
Published: March 2023

To solve the problems associated with micron-sized aluminum (Al), including sintering, agglomeration, and slag deposition during the combustion of aluminized propellants, aluminum–lithium (Al-Li) alloy, prepared by introducing a small amount of Li (1.0 wt.%) into Al, was used in place of Al. Then, the ignition and combustion characteristics of single micron-sized Al-Li alloy particles were investigated in detail using a self-built experimental apparatus and multiple characterization methods. The ignition probability, ignition delay time, flame propagation rate, burn time, combustion temperature, flame radiation spectra, and microexplosion characteristics were obtained. The TG-DSC results demonstrated that, as compared to the counterpart Al, the Al-Li alloy had a lower ignition temperature. The emission lines of AlO revealed the gas-phase combustion of the Al-Li alloy, and thus the Al-Li alloy exhibited a mixed combustion mode, including surface combustion and gas-phase combustion. Moreover, during combustion, a microexplosion occurred, which increased the combustion rate and reduced the burn lifetime. The ambient pressure had a significant effect on the ignition and combustion characteristics of the Al-Li alloy, and the ignition delay time and burn time exponentially decreased as the ambient pressure enhanced. The combustion temperature of the Al-Li alloy at atmospheric pressure was slightly higher than those at elevated pressures. The Al-Li alloy burned in N2, but no microexplosion was observed. Finally, the ignition and combustion mechanism of the Al-Li alloy in air was demonstrated by combining SEM, EDS, and XRD analyses of the material and residues. The results suggest that the addition of Li promoted the combustion performance of Al by changing the surface structure of the oxide film and the combustion mode.
Thermal Hysteresis in Melting–Solidification of Nanoparticles
Authors: Burlakov Victor M.
Published: March 2023

The aim of this paper is the development of a qualitative understanding of thermal hysteresis, namely the difference between the melting Tm and solidification Ts temperatures of nanoparticles as a function of the particle size. In contrast to the melting temperature, the determination of the absolute value of the solidification temperature for nanoparticles is generally more difficult and subjected to significant uncertainties. In this study, we implemented a very generic approach based on classical nucleation theory and define the thermal hysteresis for a nanoparticle relative to its value for a much larger ‘reference’ particle made of the same material. The obtained thermal hysteresis is found to vanish when decreasing the nanoparticle size. The approach is illustrated using the examples of gold, bismuth, and platinum nanoparticles.
Taylor Spatial Frame Behavior in High Tibial Osteotomies: A Clinical–Mechanical Study
Authors: Karamanis Nikolaos , Kermanidis Alexis T. , Spyrou Leonidas A. , Bargiotas Konstantinos , Varitimidis Sokratis , Aravas Nikolaos , Malizos Konstantinos N.
Published: March 2023

The introduction of Taylor Spatial Frame (TSF) in clinical practice allows for unique capabilities in long bone deformity corrections; however, a comprehensive understanding of its mechanical characteristics and their impact on callus formation at the osteotomy site is still unclear. The current study is concerned with the clinical application of TSF in high tibial osteotomy (HTO) and the mechanical testing of this device. Fifty-five (55) patients with symptomatic medial compartment knee osteoarthritis and varus deformity underwent open-wedge HTO with the use of TSF and were prospectively monitored with regard to callus formation pattern at the site of osteotomy. Clinical evaluation revealed that the callus formation pattern was eccentric in all patients. In addition, the experimental results from mechanical testing of a clinically relevant TSF configuration indicate, that vertical deflection of the upper bone part during weight-bearing is accompanied by a rotation of the bone axis, which acts in the same direction to the rotation applied during the clinical correction process. The complementary contributions of the deformity correction process and the mechanical response of the TSF under compressive loads, lead to asymmetric gap closure, which promotes the eccentric callus formation in the osteotomy site. The study provides useful information for clinical decision-making regarding callus formation process when TSF external fixator is applied in HTOs.