bluuebunny/crossref_metadata_2025 huggingface dataset:
Navi-imaging using depth cross-section virtual reality images and p/n inversion control: considering dimension expansion
Authors: Ogata Yoichi , Kolchiba Mykyta , Hizumi Kohki
Published: July 2023

We prototype a training-type virtual reality-head mounted display (VR-HMD) for pilots and drivers for the development of a navi-light device for vehicles. As a new challenge, we propose to reproduce VR images from one photo where a traffic accident occurred actually, and then to assist drive and flight training for driver and pilot in such a VR space. Concretely, we rendered virtual reality (VR) images from one photo using depth estimation software. A hole created in the VR images moved continuously in the stacking direction of the VR images, demonstrating the possibility of developing an application for a view-guiding device. We also controlled how the VR images were observed during the view-guiding through an inversion operation between p and n- types using a polarizer.
Integrated photonic RF self-interference cancellation on a silicon platform for full-duplex communication
Authors: Han Xiuyou , Su Xinxin , Chao Meng , Yang Xindi , Wang Weiheng , Fu Shuangling , Du Yicheng , Wu Zhenlin , Zhao Mingshan
Published: July 2023

In-band full-duplex (IBFD) technology can double the spectrum utilization efficiency for wireless communications, and increase the data transmission rate of B5G and 6G networks and satellite communications. RF self-interference is the major challenge for the application of IBFD technology, which must be resolved. Compared with the conventional electronic method, the photonic self-interference cancellation (PSIC) technique has the advantages of wide bandwidth, high amplitude and time delay tuning precision, and immunity to electromagnetic interference. Integrating the PSIC system on chip can effectively reduce the size, weight, and power consumption and meet the application requirement, especially for mobile terminals and small satellite payloads. In this paper, the silicon integrated PSIC chip is presented first and demonstrated for IBFD communication. The integrated PSIC chip comprises function units including phase modulation, time delay and amplitude tuning, sideband filtering, and photodetection, which complete the matching conditions for RF self-interference cancellation. Over the wide frequency range of C, X, Ku, and K bands, from 5 GHz to 25 GHz, a cancellation depth of more than 20 dB is achieved with the narrowest bandwidth of 140 MHz. A maximum bandwidth of 630 MHz is obtained at a center frequency of 10 GHz. The full-duplex communication experiment at Ku-band by using the PSIC chip is carried out. Cancellation depths of 24.9 dB and 26.6 dB are measured for a bandwidth of 100 MHz at central frequencies of 12.4 GHz and 14.2 GHz, respectively, and the signal of interest (SOI) with 16-quadrature amplitude modulation is recovered successfully. The factors affecting the cancellation depth and maximum interference to the SOI ratio are investigated in detail. The performances of the integrated PSIC system including link gain, noise figure, receiving sensitivity, and spurious free dynamic range are characterized.
Plasmon resonance-enhanced graphene nanofilm-based dual-band infrared silicon photodetector
Authors: Liu Xinyu , Wu Shaoxiong , Cao Xiaoxue , Tian Feng , Bodepudi Srikrishna Chanakya , Malik Muhammad , Gao Chao , Peng Li , Hu Huan , Xu Yang
Published: July 2023

Graphene-based photodetectors have attracted much attention due to their unique properties, such as high-speed and wide-band detection capability. However, they suffer from very low external quantum efficiency in the infrared (IR) region and lack spectral selectivity. Here, we construct a plasmon-enhanced macro-assembled graphene nanofilm (nMAG) based dual-band infrared silicon photodetector. The Au plasmonic nanostructures improve the absorption of long-wavelength photons with energy levels below the Schottky barrier (between metal and Si) and enhance the interface transport of electrons. Combined with the strong photo-thermionic emission (PTI) effect of nMAG, the nMAG–Au–Si heterojunctions show strong dual-band detection capability with responsivities of 52.9 mA/W at 1342 nm and 10.72 mA/W at 1850 nm, outperforming IR detectors without plasmonic nanostructures by 58–4562 times. The synergy between plasmon–exciton resonance enhancement and the PTI effect opens a new avenue for invisible light detection.
Genetic diversity and drug sensitivity profile of Mycobacterium tuberculosis among children in Ethiopia
Authors: Mollalign Hilina , Getahun Muluwork , Diriba Getu , Alemu Ayinalem , Chala Dawit , Tulu Begna , Ameni Gobena
Published: July 2023

Background

Worldwide, tuberculosis (TB) affects about one million children every year. The burden of the disease is higher in developing countries. However, there is limited information on the lineages and drug sensitivity patterns of Mycobacterium tuberculosis (M. tuberculosis) infecting children in these countries, including Ethiopia. Thus, this study aimed to characterize the different lineages of the M. tuberculosis complex causing childhood pulmonary tuberculosis and evaluate the drug-sensitivity patterns to the first-line anti-TB drugs.

Method

A total of 54 stored cultures were used in this study. The region of difference 9 (RD9) based polymerase chain reaction (PCR) and spoligotyping were employed for the identification of the isolates at the species and lineages level respectively. Lineage identification was done by using the pre-existing database. Identification of clustering of the spoligotype patterns was by using the SPOLIDB3-based model. The result was retrieved by the most probable family format. Furthermore, the phenotypic, and genotypic drug-sensitivity test (DST) was performed using Mycobacterium Growth Indicator Tube (MGIT™ 960) and GenoTypeMTBDRplus assay respectively. Data analysis was done using SPSS version 27 software.

Result

Spoligotyping produced 39 interpretable results for M. tuberculosis. The majority (74.4%) of them were clustered into 7 groups, while the rest (25.6%) were single. The Euro-American (EA) lineage was the predominant lineage (64.1%) followed by the East-African Indian (EAI) (30.8%) and M. Africanum (5.1%) lineages. The most predominant subtypes were SIT37 (15.4%), SIT149 (12.8%), SIT25 (7.7%), and SIT53 (7.7%). Furthermore, of the identified SITs, T1 and CAS families consisted of 38.5% and 28.2% of the lineages respectively. Drug susceptibility was 91.9% by phenotypic method and 97.4% by molecular assay. The overall prevalence of any resistance was 7.8% and there was a single MDR-TB.

Conclusion

Many of the isolates belong to the modern lineages (Euro American) representing the most common circulating strains in the country. More importantly, despites the tiny isolates tested, drug resistance is low. To fully describe the molecular epidemiology of MTBC lineages in children, we recommend a prospective large-scale study.
Epidemiological clinical profile and closure of chronic plantar ulcers in patients with leprosy sequelae undergoing orthopedic surgery in a municipality in western Amazon
Authors: João Francisco Mateus , Peninni Silmara Navarro , Vasconcelos Zanair Soares , da Silva Arineia Soares , Couceiro Katia do Nascimento , Jorge Brandão Alba Regina , Silva Mônica Regina Hosannah da Silva e , do Vale Filho Marcello Facundo , de Oliveira Guilherme Miranda Silva , Ferreira Lucas Silva , Mwangi Victor Irungu , da Silva Bernardo Maia , Barbosa Guerra Maria das Graças Vale , Guerra Jorge Augusto de Oliveira
Published: July 2023

Introduction

Chronic plantar ulcers in leprosy are lesions resulting from motor and sensory alterations caused by Mycobacterium leprae. They are lesions refractory to conventional dressings and present high recurrence rates.

Objective

To evaluate the epidemiological clinical profile of patients with chronic plantar ulcers associated with bony prominences in the lesion bed and to evaluate the efficacy of orthopedic surgical treatment of these lesions.

Methods

This is a descriptive and analytical retrospective study with the evaluation of medical records of patients undergoing surgical treatment of chronic plantar ulcers from 2008 to 2018. The surgical technique applied consisted of corrective resection of bone prominences and the primary closure of the lesion with bipediculated local flap.

Results

234 patients were submitted to surgery, 55.1% male with an average age of 69.5 years old. Of these, 82.9% were illiterate; and 88.5% with open lesions over 10 years. After surgical treatment, total wound healing occurred in an average time of 12 weeks. The variables that contributed to shorter healing time were: Patients’ lower age group; regular use of orthopedic shoes and insoles and dressings performed by nurse aides in health units before surgery. Obesity was the factor that correlated with the delay of healing time.

Conclusion

A higher incidence was observed in males and male and female illiterate patients. The regular use of shoes and insoles and dressings performed by nurse aides in health units contributed to shorter postoperative healing time. Orthopedic surgical treatment with corrective resection of bony prominences proved to be an efficient therapeutic method for the closure of chronic plantar ulcers. It is a reproducible method, justifying the importance of the orthopedic surgeon in the context of the multidisciplinary team to cope with these complex lesions.
Effect of the INTER-ACT lifestyle intervention on maternal mental health during the first year after childbirth: A randomized controlled trial
Authors: Van Uytsel Hanne , Ameye Lieveke , Devlieger Roland , Bijlholt Margriet , Van der Gucht Katleen , Jacquemyn Yves , Bogaerts Annick
Published: July 2023

We assess whether the INTER-ACT postpartum lifestyle intervention influences symptoms of depression and anxiety, sense of coherence and quality of life during the first year after childbirth. A total of 1047 women of the INTER-ACT RCT were randomized into the intervention (n = 542) or control arm (n = 505). The lifestyle intervention consisted of 4 face-to-face coaching sessions, supported by an e-health app. Anthropometric and mental health data were collected at baseline, end of intervention and 6-months follow-up. We applied mixed models to assess whether the evolution over time of depressive symptoms, anxiety, sense of coherence and quality of life differed between the intervention and control arm, taking into account the women’s pre-pregnancy BMI. There was no statistical evidence for a difference in evolution in anxiety or quality of life between intervention and control arm. But an improvement in symptoms of depression and sense of coherence was observed in women who received the intervention, depending on the mother’s pre-pregnancy BMI. Women with normal/overweight pre-pregnancy BMI, reported a decrease in EPDS between baseline and end of intervention, and the decrease was larger in the intervention arm (control arm: -0.42 (95% CI, -0.76 to -0.08); intervention arm: -0.71 (95% CI, -1.07 to -0.35)). Women with pre-pregnancy obesity showed an increase in EPDS between baseline and end of intervention, but the increase was less pronounced in the intervention arm (control arm: +0.71 (95% CI, -0.12 to 1.54); intervention arm: +0.42 (95% CI -0.42 to 1.25)). Women with a normal or obese pre-pregnancy BMI in the intervention arm showed a decrease in sense of coherence between baseline and end of intervention (-0.36) (95% CI, -1.60 to 0.88), while women with overweight pre-pregnancy showed an increase in sense of coherence (+1.53) (95% CI, -0.08 to 3.15) between baseline and end of intervention. Receiving the INTER-ACT postpartum lifestyle intervention showed improvement in depressive symptoms, in normal weight or overweight women on the short run, as well as improvement in sense of coherence in women with pre-pregnancy overweight only.

Trial registration:ClinicalTrials.gov;NCT02989142.
Recreational and sexualised drug use among gay, bisexual, and other men who have sex with men (gbMSM) in Ireland–Findings from the European MSM internet survey (EMIS) 2017
Authors: Daly Fionn P. , O’Donnell Kate , Davoren Martin P. , Noone Chris , Weatherburn Peter , Quinlan Mick , Foley Bill , Lyons Fiona , Igoe Derval , Barrett Peter
Published: July 2023

Background

Gay, bisexual, and other men who have sex with men (gbMSM) report a higher prevalence of drug use in comparison to the general male population. However, in Ireland, there is a paucity of literature regarding the prevalence of drug use and its determinants among gbMSM.

Aims/Objectives

To quantify the prevalence of (i) recreational drug use (RDU) and (ii) sexualised drug use (SDU) among gbMSM in Ireland, and to identify the factors associated with these drug use practices.

Methods

The European MSM Internet Survey (EMIS) 2017 was an online, anonymous, internationally-promoted questionnaire. Two binary outcomes were included in our analyses: (1) RDU and (2) SDU in the previous year. Multivariable-adjusted logistic regression explored factors associated with these outcomes, and all independent covariates were adjusted for one another.

Results

Among gbMSM without HIV (n = 1,898), 40.9% and 13.1% engaged in RDU and SDU in the previous year, respectively. Among diagnosed-positive gbMSM (n = 141), the past-year respective prevalence estimates were 51.8% and 26.2%. Increased odds of RDU were observed among gbMSM who were younger (vs. 40+ years) (18–24 years; AOR 2.96, 95% CI 2.05–4.28, 25–39 years; AOR 1.66, 95% CI 1.27–2.16), lived in Dublin (vs. elsewhere) (AOR 1.47, 95% CI 1.17–1.83), and engaged in condomless anal intercourse (CAI) in the previous year (vs. none) (1–2 partners; AOR 1.79, 95% CI 1.34–2.38, 6+ partners; AOR 1.79, 95% CI 1.18–2.71). Greater odds of SDU were identified among those who lived in Dublin (vs. elsewhere) (AOR 1.50, 95% CI 1.07–2.10), and engaged in CAI (vs. none) (1–2 partners; AOR 3.16, 95% CI 2.05–4.88, 3–5 partners; AOR 2.50, 95% CI 1.47–4.26, and 6+ partners; AOR 3.79, 95% CI 2.23–6.43).

Conclusion

GbMSM report a high prevalence of drug use in Ireland. Targeted interventions, including harm reduction campaigns, may be needed to support healthier drug use choices among this community.
Impact of body composition on patient prognosis after SARS-Cov-2 infection
Authors: Yamamoto Takayuki , Sugimoto Kazushi , Ichikawa Syuhei , Suzuki Kei , Wakabayashi Hideki , Dohi Kaoru , Yamamoto Norihiko
Published: July 2023

Background

Since the first outbreak of coronavirus disease 2019 (COVID-19), it has been reported that several factors, including hypertension, type 2 diabetes mellitus, and obesity, have close relationships with a severe clinical course. However, the relationship between body composition and the prognosis of COVID-19 has not yet been fully studied.

Methods

The present study enrolled 76 consecutive COVID-19 patients with computed tomography (CT) scans from the chest to the pelvis at admission. The patients who needed intubation and mechanical ventilation were defined as severe cases. Patients were categorized into four groups according to their body mass index (BMI). The degree of hepatic steatosis was estimated by the liver/spleen (L/S) ratio of the CT values. Visceral fat area (VFA), psoas muscle area (PMA), psoas muscle mass index (PMI), and intra-muscular adipose tissue content (IMAC) were measured by CT scan tracing. These parameters were compared between non-severe and severe cases.

Results

Severe patients had significantly higher body weight, higher BMI, and greater VFA than non-severe patients. However, these parameters did not have an effect on disease mortality. Furthermore, severe cases had higher IMAC than non-severe cases in the non-obese group.

Conclusions

Our data suggest high IMAC can be a useful predictor for severe disease courses of COVID-19 in non-obese Japanese patients, however, it does not predict either disease severity in obese patients or mortality in any obesity grade.
Reseña de: Macari, Mirko (2023). Sr. Director: Memorias desde el antiperiodismo. Planeta.
Authors: Mayol Alberto
Published: July 2023

Mirko Macari ha escrito un libro que examina fundamentalmente la posdictadura y su práctica en los medios de comunicación. El fin de la dictadura entregó un Chile con numerosas revistas disidentes, pero con pocos periódicos. En los primeros años de la transición existió un medio llamado “La Época”, pero muy poco duraría. En esa escena ningún medio logró una influencia en las elites por varios años. De hecho, el único medio escrito que logró penetrar la sala de máquinas del poder político fue El Mostrador y específicamente en la época en que Mirko Macari, el autor de este libro, fue su Director...
Assessing Educational Mismatch in the Spanish Hospitality Industry
Authors: Sánchez-Cubo Francisco , Mondéjar-Jiménez José , García-Pozo Alejandro
Published: July 2023

For decades, academics have studied the human resources of many different sectors with a wide range of approaches and methodologies. However, there is still room for analysis on educational mismatch, even more so in the hospitality industry after the COVID-19 pandemic standstill as it is a topic that has been somewhat relegated in recent times. Thus, this study seeks to analyze this phenomenon based on a multinomial logistic regression, which is underused in the human capital-related literature, especially on educational mismatch. The results confirm the influence of several factors such as tenure, experience or skills and quantify their effects on the chances of being educationally mismatched. Moreover, special attention is given to the remarkable influence of the required skills for a specific job position on vertical mismatch. Therefore, this paper updates previous research on the issue but also sheds new light on identifying the main aspects in which employees may focus in order to have better career prospects. The main limitation of this study is the availability of the data, which corresponds to 2018.
Turismo sexual y territorio: el espacio vivido de las sexoservidoras en Veracruz-Boca del Río
Authors: Gallegos Jiménez Oswaldo , López López Álvaro
Published: July 2023

La imagen turística predominante del área urbana Veracruz-Boca del Río descansa en sus atractivos físicos, culturales, históricos y su función portuaria, y no tanto en aspecto sexuales; sin embargo, aunque soterrada, este corredor turístico tiene una dinámica significativa de turismo sexual, derivada de la liminalidad que le imprimen los espacios turísticos litorales y la propia algarabía local. Así, este estudio analiza, desde una perspectiva geográfica de base cualitativa —centrada en la observación no participante y participante, así como en entrevistas a profundidad—, la forma en que las mujeres sexoservidoras —cisgénero y transgénero—se apropian, organizan, transforman y perciben el territorio, en el contexto de la dinámica turística local. En este trabajo, el fenómeno del turismo sexual se reconoce a partir de que las sexoservidoras identifican que algunos de sus clientes son turistas o visitantes a Veracruz-Boca del Río; además, desde su perspectiva del espacio vivido, se explora el fenómeno del turismo sexual.
Predicción del comportamiento del consumidor en destinos turísticos
Authors: Pérez Ricardo Elizabeth del Carmen , Batista Sánchez Ernesto , Coronado Provance Katia Zucel , Medina Labrada Justa Ramona , Feria Velázquez Francisco Fidel
Published: July 2023

La presente investigación tiene como objetivo proponer un modelo teórico, partiendo de la literatura previa, que incluya los antecedentes de la intención de comportamiento del consumidor hacia un destino turístico y explore las relaciones entre variables. A tal fin se utilizó un Modelo de Ecuaciones Estructurales basado en datos obtenidos mediante tres cuestionarios online, que fueron aplicados a consumidores de tres destinos turísticos. A partir del análisis factorial confirmatorio y la evaluación del modelo estructural se determinó la relación existente entre las variables observadas y variables latentes, así como el grado de sensibilidad de cada una. Los resultados permitieron corroborar las hipótesis planteadas y revelaron como antecedentes que más influyen en la intención de comportamiento futuro (1) las actitudes del consumidor, (2) los atributos del destino, (3) el rol mediador de la satisfacción y (4) el rol moderador de la personalidad. Asimismo, los hallazgos mostraron los factores que se encuentran más afectados en cada destino y los que mayor peso tienen en la intención de repitencia del consumidor. El modelo teórico presentado contribuye a la literatura existente sobre comportamiento del consumidor turístico e identifica los principales antecedentes que influyen en la intención de comportamiento hacia un destino turístico. El valor de este estudio radica en el análisis de las relaciones entre las variables latentes vinculadas a la intención de comportamiento, identificando sus antecedentes, así como variables mediadora y moderadora. Además, aporta información oportuna para la toma de decisiones estratégicas de los gestores turísticos en estos destinos.
Orbitopatía tiroidea. Puntos clave para el diagnóstico y tratamiento
Authors: Marqués-Fernández Victoria Eugenia , Galindo-Ferreiro Alicia , Palacios-Mures Jose María , Villacorta-Martín María del Mar
Published: July 2023

Objetivo: La orbitopatía tiroidea (OT) es una patología autoinmune de etiología desconocida. Se trata de una enfermedad edematosa e inflamatoria crónica y a veces subaguda o aguda, cuyas características principales son edema palpebral, úlceras corneales, hipertensión ocular, exoftalmos generalmente bilateral, estrabismo restrictivo, diplopía y neuropatía óptica. El objetivo principal es realizar una revisión y actualización acerca del enfoque diagnóstico y terapéutico de la OT. Realizar una revisión y actualización acerca del enfoque diagnóstico, terapéuticos de la OT. Métodos: Revisión de la literatura publicada referente a la OT y manera actual de enfocar esta patología. Resultados: La OT es una enfermedad inflamatoria orbitaria con un posible origen autoinmune y que suele asociarse a trastornos metabólicos de la glándula tiroidea. Fisiopatológicamente su mecanismo no está claro. Afecta mayormente a mujeres y está negativamente influenciada por factores como el tabaquismo, la edad, el sexo y la raza. Es imprescindible clasificarla en cuanto a su severidad y su actividad para un correcto manejo. Conclusiones: El conocimiento clínico de OT es esencial para el diagnóstico precoz de la enfermedad. El tratamiento médico, en caso de OT activa, debe ser precoz, agresivo y acorde a la fase en que se presente el paciente con el fin de evitar las graves consecuencias de la OT. El tratamiento quirúrgico deberá ser lógico y ordenado, pero a su vez rápido, con el fin de devolver al paciente al entorno laboral, social y familiar.
A lipidek szerepe az ősziárpa fényindukált hidegakklimatizációjában
Authors: Kovács Terézia
Published: July 2023

Az ősszel vetett gabona 30-40% -kal magasabb hozamot biztosít a tavasziakhoz képest. Azonban, ezeknek a növényeknek át kell vészelniük a telet. A növények télállósága döntően fagytűrésüktől függ. A fagyállóság kialakulása genetikailag meghatározott folyamat, melyet befolyásol a hőmérséklet, a nappal hossz és a növényeket megvilágító fény spektruma. Előző kutatásokból ismert, hogy mind az Arabidopsis modell növény esetében, mind a gabonaféléknél a fehér fény távoli vörös fénnyel való kiegészítése növeli a fagyállóságot viszonylag magas hőmérsékleten (15 ˚C) is. Közismert az is, hogy a hideg-akklimatizáció során a membrán lipid összetételének megváltozása összefügg a fagyállósággal. Ezeknek az eredményeknek a döntő többsége Arabidopsis kísérletekből származik. A gabonafélék, így az árpa esetében is, a membrán lipid összetétel változásáról kevés az információ. Arról pedig egyáltalán nincs eredmény közölve, hogy a vörös távoli vörös fény valamint a kék fény arányának megváltozása hogyan befolyásolja a növények membrán lipid összetételét. Ezért megvizsgáltuk, hogy a hideg kezelés kombinálása a spektrum változással hogyan befolyásolja az árpa levelek lipid összetételét, és a lipid bioszintézist meghatározó gének expresszióját. Jelentős változást tapasztaltunk a HexCer, a LysoPC, az MGDG és az MGDG/DGDG lipid osztályok esetében. Az MGDG2, a DGDG, az NC, az LLO2, a LOC és az AD3 gének expressziója megemelkedett már 15 ˚C-on a fehér fény távoli vörössel való kiegészítése következtében. A hideg akklimatizáció és a megvilágítás által indukált lipid változás jól korrelál a fagy tolerancia kialakulásával.
A Histone H4 replacement (H4r) gén funkciójának vizsgálata Drosophila melanogasterben
Authors: Ábrahám Andrea
Published: July 2023

Eukarióta sejtekben a DNS sejtmagba történő becsomagolását a hiszton fehérjék végzik. A hisztonok és DNS komplexeként létrejövő kromatin kondenzációján keresztül ezek a fehérjék a genom stabilitásáért és a génműködés szabályozásáért felelősek. A DNS replikációjához kötötten kifejeződő kanonikus hisztonok minden sejtben megtalálhatók és poszt-transzlációs módosításaiktól függő módon vesznek részt az érintett régiók működésének szabályozásában. Ezzel szemben a replikációfüggetlen expressziójú hiszton variánsok a génexpresszió szabályozásában specifikus funkciókkal rendelkeznek, és mutathatnak sejttípusspecifikus expressziót. A Drosophila melanogaster kanonikus hiszton génjei egy klaszterben helyezkednek magas kópiaszámban. Ezzel szemben a hiszton variánsok a hiszton klaszteren kívül helyezkednek el a genomban, egy példányban, szerkezetükben általában kissé eltérnek kanonikus megfelelőiktől, specifikus szabályozásuk pedig lehetővé teszi specifikus szöveti vagy differenciálódási funkciók ellátását. A többi alternatív hisztontól eltérően a H4 variáns fehérje (H4r) aminosavsorrendje megegyezik a kanonikus H4-ével. Annak érdekében, hogy a H4r-t megkülönböztethessük a kanonikus H4-től és információt nyerhessünk a szerepéről, epitóp-jelöltük a H4r-t, tanulmányoztuk annak térbeli és időbeli kifejeződését, és feltártuk a kromatinban való lokalizációját nukleoszomális szinten. Immunhisztokémiai vizsgálatok alapján a H4r általánosan kifejeződik az embrionális és lárvális fejlődési állapotokban, de a fejlődő idegrendszerben, a H4r expressziója tekintetében enyhe eltérések figyelhetők meg az egyes sejttípusok között, a H4r az agyi sejtek egy kisebb populációjában felhalmozódást mutat, mely nem korlátozódik sejttípusra vagy differenciációs stádiumra. ChIP-seq kísérletekkel kimutattuk, hogy a H4r lokalizációja preferenciális összefüggést mutat a szabályozó régiókkal, különösen a sejtek stresszre adott válaszaiban részt vevő gének esetén. Mindent összevetve a kísérleti adatok azt jelzik, hogy a H4r-nek van a kanonikus H4-től eltérő, specifikus variáns hiszton funkciója, amellyel hozzájárulhat a nem osztódó sejtek transzkripciós memóriájának kialakításához.
Macro-organization of photosynthetic complexes during salt stress acclimation of green microalgae and in plant chloroplast acetyltransferase and Ser/Thr protein kinase mutants
Authors: Kanna Sai Divya
Published: July 2023

Doktori munkám során célom volt tanulmányozni sóstressz hatására bekövetkezett változásokat i) a Chlamydomonas reinhardtii (C. reinhardtii) fotoszintetikus apparátus szerkezetében és működésében ii) az Euglena gracilis (E. gracilis) akklimatizációs reakcióiban, továbbá iii) feltárni a proteinek acetilezésének/ foszforilálásának szerepét az Arabidopsis thaliana (A. thaliana) fotoszintetikus apparátusának membrándinamikájában. A sóstressz negatív hatással volt az E. gracilis sejtek növekedésére és klorofill tartalmára, valamint kihatott a TM makro-szerveződésére, de nem volt hatása a fotoszintetikus folyamatokra és a fotoszintetikus komplexek összetételére. Ugyanakkor a karotinoid-klorofill arány és a biotechnológiai szempontból jelentős paramilon tartalom megnőtt. A raktározott poliszacharid felhalmozódása, a pigment összetételben és a TM szerveződésében bekövetkező változások segíthetik az E. gracilis sejtek sóstresszhez történő alkalmazkodását. Vizsgáltuk a sóstressz hatását a C. reinhardtii vad típusú és két stt7 és a pgrl1 mutáns törzsére. Kimutattuk, hogy a sóstressz kihatott a tilakod membránok (TM) ismétlődési távolságára; a pigment-fehérje komplexek szerveződésére és működésére. Az stt7 mutáns fokozott sóstressz érzékenysége, arra utal, hogy a fotoszintetikus állapotátmenetek kulcsszerepet játszanak a sóstresszhez való alkalmazkodásban. Vizsgáltuk a kloroplaszt acetil-transzferáz (GNAT2) és az STN7 kináz szerepét A. thaliana TM szerveződésében. A CD spektroszkópiai vizsgálatok a vad típushoz képest eltérést mutattak a PSII-LHCII szuperkomplex és/vagy az LHCII szerkezetében és elrendezésében mind a gnat2, és az stn7 mutánsokban. Ezek az eredmények megerősítették, hogy a GNAT2 az STN7 kinázhoz hasonlóan részt vesz a TM makroszervezésének alakításában. Általánosságban elmondható, hogy a membrán dinamikus szerveződése kulcsszerepet játszik a fotoszintetikus organizmusok stressz-adaptációjában, melyben a fehérjék poszttranszlációs módosításának is szerepe lehet.
Electrochemical Synthesis of Efficient Catalyst Ni-Fe on Ni Foam for Electrochemical Water Splitting
Authors: Mo Nguyen Thi , Chau Nguyen Minh , Bach Nguyen Minh , Duc Pham Quang , Hung Hoang Van
Published: July 2023

Herein, the Ni-Fe electrocatalysts dispersed on nickel foam (NF) were successfully synthesized by the electrodeposition method. The electrode materials were characterized by XRD, EDX and SEM techniques. Electrochemical analysis was performed to determine the electrocatalytic properties for oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) of the overall water splitting. The results indicate that the NiFe/NF electrode exhibits superior electrocatalytic activity as compared to the Ni/Fe and Fe/NF electrodes for both OER and HER with a significantly lower overpotential, 300 mV to reach 10 mA cm–2 with OER and 364 mV to reach −20 mA cm–2 with HER.
A New Validated Method for Simultaneous Determination of Aerodynamic Particle Size Distribution of Drug Particles of Mometasone Furoate and Formoterol Fumarate Dihydrate in 200 mcg/5 mcg Inhalation Aerosol
Authors: Prakash M. Surya , Subbaiah B. Venkata , Annapurna N.
Published: July 2023

Simultaneous determination of aerodynamic particle size distribution (APSD) of drug particles of mometasone furoate and formoterol fumarate dihydrate – 200 mcg/5 mcg inhalation aerosol (MDI) has been developed and reported in this work. The method was developed in consistent with USP <601> inhalation and nasal drug products e.g. aerosols, sprays and powders. The next generation impactor (NGI) was used and operated at 30 ± 5% L/min. The proposed method was demonstrated for sensitivity, minimum number of actuations, diluent volume optimization, mass balance and precision. The proposed method was found to be robust, accurate, linear and precise.
Spectroscopic and Electrochemical Investigation of Peripherally Diruthenated Complexes of octakis(benzylthio) and (methylthio)tetraazaporphyrinmagnesium(II)
Authors: Kumar Ajay , Kumar Rajeev , Rastogi Arshi
Published: July 2023

Four new trinuclear complexes were successfully synthesized through the dative binding of one unit of (bpy)2RuII and one unit of (phen)2RuII to the periphery of octakis(benzylthio)tetraazaporphyrinmagnesium(II) [Mg(OBTTAP)] and octakis(methylthio)tetraazaporphyrinmagnesium(II) ([Mg(OMTTAP)]). Spectroscopic techniques were used to perform an extensive characterization of the complexes, including an analysis of their fluorescence excitation/emission spectra and redox behaviours. Effective electronic interaction between the peripheral (bpy)2RuII/(phen)2RuII and [Mg(OBTTAP)] units is evidenced by the presence of these complexes. In cyclic voltammetry, the oxidation waves were slightly altered to a higher positive potential upon attaching a second Ru(II) unit in complexes 6, 7, 8 and 9.
Chemical Composition and in vitro Biological Evaluation of Essential Oil of Euphorbia larica Boiss from Northern Oman
Authors: Meenakshi Dhanalekshmi Unnikrishnan , Alam Tanveer , Zafarullah Roshna , Khan Shah Alam
Published: July 2023

Euphorbia larica Boiss is a wild plant of Oman that grows in gravel plains, mountain areas, desert and forest edges. Traditionally, Omani herbalists use the Euphorbia larica plant extract, resins, latex and juice to treat a wide range of ailments such as wounds, burns, insect bites, intestinal parasites, gonorrhea, eye infections, migraines and warts. In an attempt to scientifically validate the traditional uses and to unlock its hidden therapeutic potential, in this work, the chemical composition of E. larica essential oil (ELEO) along with its potential antioxidant, antimicrobial and hemolytic activities is studied. In a GC-MS analysis of the extracted ELEO, 29 chemicals were found, with α-pinene (27.36%) and limonene (11.5%) being the two most abundant volatile components. ELEO exhibited moderate inhibition of DPPH radicals (17.74-40.96%; IC50 = 48.97 μg/mL). It showed antimicrobial activity against S. aureus, B. subtilis, S. pyogenes, E. coli, P. aeruginosa and P. vulgaris but was ineffective against K. pneumoniae bacterial strain however, exhibited the maximum antibacterial activity against S. aureus (11 ± 1.3 and 15 ± 2.3 mm at 5 and 10 μL concentrations, respectively) comparable to positive antibiotic ampicillin (15 mm at 5 μg). Moreover, ELEO exhibited a very low hemolytic activity (0.12 to 1.1% at doses of 50-500 μg) suggesting it to be non-toxic to human or animal cells. Thus, it can be concluded that E. larica plant holds significant potential as a source of bioactive agents for the development of novel therapeutics.
Corrosion Inhibition Potential of Cymbopogon citratus (Lemongrass) Leaves Extract on Mild Steel in 0.1 M Sulphuric Acid Medium at Different Temperatures
Authors: Nunach Amit Kumar , Rani Gita , Ahlawat Rachna
Published: July 2023

The role of the phytochemicals in Cymbopogon citratus (Lemongrass) plant is significant in preventing the corrosion. The current study investigated the effectiveness of lemongrass leaves extract (at concentrations ranging from 1-6%) in inhibiting the corrosion of mild steel (low carbon) in a corrosive medium of 0.1 M sulphuric acid. The extract was used as a green rust inhibitor at different temperatures (25, 35 and 45 ºC). Electrochemical impedance spectroscopy (EIS) and linear polarization studies were conducted to analyze the electrochemical behaviour and understand the corrosion inhibition mechanism. Additionally, weight loss studies were carried out. At 6% concentration and 25 ºC, lemongrass extract showed 92.01% corrosion inhibition efficiency. The surface analysis of mild steel samples was performed using both metallurgical research micrographs (MRM) and SEM techniques. Based on the results, the study concludes that the lemongrass leaves extract is an excellent green corrosion inhibitor.
Evaluation of Free Radical Quenching Ability of Quinoline Acids through in vitro and Theoretical Studies
Authors: Kiruthika S. , Sharulatha V.
Published: July 2023

Drugs based antioxidants are commonly suggested as targets while designing new medicines. All sorts of pharmaceutical drug development benefit from the extremely desirable quinoline moiety with antioxidant. In this work, the scavenging behaviour of four quinoline derivatives (Q1-Q4) towards DPPH, H2O2, ABTS and superoxide activity were investigated. According to the in vitro inhibition concentration (IC50), quinoline derivative Q1 showed high antioxidant potential. Additionally, the theoretical DFT gas phase calculations of HOMO-LUMO, MEP, NPA and NBO are used to study the conjugating systems in radicals and showed that the N-H site acts more favourable than the O-H site for the radical attack. The calculated bond dissociation energy (BDE) values demonstrated that compound Q1 follows the HAT mechanism and while the calculated ionization potential (IP) and proton dissociation energy (PDE) values showed that Q4 follows the SET-PT route. The results of these two mechanisms demonstrated that radical quenching activity occurs at the N-H and O-H sites. The spin density demonstrates that both radicals are delocalized uniformly across the molecule.
Synthesis and DFT Study of 7-Bromophenylnaphthopyran Moieties
Authors: El-Wahab Ashraf Hassan Fekry Abd , Mohamed Hany Mostafa
Published: July 2023

A one-pot, three-component reaction of 6-bromo-2-naphthol (1), p-chlorobenzaldehyde (2) and malononitrile or ethyl cyanoacetate (3) in ethanol/piperidine under reflux was performed to afford 4H-naphtho[2,1-b]pyrano-3-carbonitrile (4a) and ethyl 4H-naphtho[2,1-b]pyrano-3-carboxylate (4b) derivatives, respectively. The structure of these compounds was determined using IR, 1H NMR, 13C NMR, mass spectroscopy and UV-Vis spectra. The molecular geometry of compounds 4a and 4b was determined at the B3LYP/631+G(d) level. The geometric optimization was performed on two tautomers and two conformers. Tautomers were separated by about 7.942 kcal/mol, while rotational conformers were separated by just 0.511 kcal/mol. The global electrophilicity, hardness, softness and local condensed Fukui functions were calculated and considered as molecular reactivity descriptors, moreover the frontier molecular orbitals (HOMO and LUMO) were also calculated.
Synthesis of Novel Fluorinated 5-Benzylidine-3-ethyl-2-(2,3,4-trifluorophenylimino)thiazolidin-4-one Derivatives using Knoevenagel Reaction and Evaluation of their in vitro Antimicrobial Potentials
Authors: Kadam S.D. , Mammen D. , Kadam D.S. , Patil S.G. , Bagul R.R. , Doshi A. , Patel F.
Published: July 2023

A series of novel 5-benzylidine-3-ethyl-2-(2,3,4-trifluoro-phenylimino)-thiazolidin-4-one derivatives were synthesized by Knoevenagel reaction using both conventional as well as non-conventional methods on the synthesized iminothiazolidinone core. The results of an ultrasonic Knoevenagel reaction showed that it was more efficient than the traditional method in terms of yield and reaction time. The compounds were characterized using 1H, 19F and 13C NMR spectra, supported by mass spectrometric and infrared spectroscopic data. The stereochemistry of the final compounds was confirmed using 2D NOESY NMR experiment. The in vitro antibacterial potential of the synthesized benzylidine derivatives were also evaluated. Compounds having trifluoromethyl group on benzylidine moiety were observed to be the most active against the tested bacterial strains as compared to the rest of the compounds.
Effect of Nanoalumina on Physical and Thermal Properties of Polypropylene-Glass Fiber Reinforced Nanocomposites
Authors: Raghvan Sonu , Singhal Prachi , Rattan Sunita , Tyagi Ajay Kumar
Published: July 2023

The present study examines the effect of nanoalumina on the mechanical and thermal properties of glass fiber and elastomer reinforced PP/EPDM/GF/Al2O3 with an aim to develop suitable composite material for orthotics. In present study, PP/GF, PP/EPDM/GF composite and PP/EPDM/GF/Al2O3 nanocomposites with specific composition were fabricated using melt-blending technique. Prepared composites were characterized through scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The mechanical characteristics including tensile strength, tensile modulus, notched Izod impact strength and elongation at break of the prepared composite were studied in accordance with ASTM guidelines. PP/EPDM/GF/Al2O3 composites showed the mechanical properties of PP/EPDM/GF/Al2O3 composites showed 62%, 145%, 15% and 165% enhancement in tensile strength, tensile modulus, notched Izod impact strength and elongation at break, respectively as compared to PP/GF composites. The mechanical properties and thermal properties of PP/EPDM/GF/Al2O3 were also compared with PP/EPDM/GF composites. The use of nanoalumina significantly and brilliantly balances the complimentary tensile and elongation properties in PP/EPDM/GF/Al2O3 composite, which is an essential requirement for fabricating composite material capable of being thermoformed to orthotic devices.
АНАЛИЗ ДАННЫХ РЕГИСТРА ПАЦИЕНТОВ С АКРОМЕГАЛИЕЙ В МОСКОВСКОЙ ОБЛАСТИ
Authors: Метлушко Е. Д. , Захарова П. А. , Иловайская И. А.
Published: July 2023

ЦЕЛЬ: оценить количество пациентов, активно наблюдающихся в областном специализированном

центре; определить частоту достижения контроля акромегалии; а также оценить долю пациентов, «потерянных» из наблюдения.

МАТЕРИАЛЫ И МЕТОДЫ: было проведено ретроспективное наблюдательное исследование. Для анализа данных были использованы 327 медицинских карт пациентов с акромегалией, наблюдающихся в ГБУЗ

МО МОНИКИ им. М.Ф. Владимирского (МОНИКИ). Критериями включения в исследование были: наличие

информативной записи эндокринолога КДЦ МОНИКИ с указанием активности акромегалии и проводимого

лечения. Критерием исключения было отсутствие визитов пациента в КДЦ МОНИКИ с 2012 г.

РЕЗУЛЬТАТЫ: среди 327 пациентов с акромегалией, наблюдающихся в МОНИКИ, средний возраст

пациента на момент установки диагноза составил 49±14,1 лет, на момент последнего визита пациента -

58±13,4 лет.

В течение последних 5 лет находились под регулярным наблюдением 199 (60,9%) пациентов, из которых

были прооперированы 63,8% пациента, получили медикаментозное лечение 69,3% пациентов (в том числе

лечение аналогами соматостатина как первой линией лечения - 29,6%), лучевое лечение – 19,6%. У 117/199

(58,8%) пациентов достигнут контроль заболевания, у 82/199 (41,2%) акромегалия неконтролируемая.

В группе контролируемой акромегалии по сравнению с неконтролируемой была статистически значимо

больше доля прооперированных пациентов (68,4% против 57%), а также доля пациентов, получавших другие методы лечения, помимо аналогов соматостатина, после проведенного нейрохирургического лечения

(33,3% против 10,9%), в то время как частота применения других видов лечения статистически значимо

не различалась в зависимости от контроля заболевания.

Более 5 лет не находятся под наблюдением 128/327 (39,1%) пациентов, из которых 14 человек умерло,

из оставшихся на момент последнего визита у 55/114 (48,2%) акромегалия была контролируемая, и у 59/114

(51,8%) - отсутствие контроля. Доля пациентов с неконтролируемой акромегалией, «потерянных» из наблюдения составляет 18% (59/327).

ВЫВОДЫ: доля пациентов, активно наблюдающихся в течение 5 лет в специализированном центре, составила 60,9%, среди которых контроль акромегалии достигнут у 58,8% пациентов. Рекомендуется рассматривать применение нейрохирургического вмешательства у пациентов с акромегалией во всех возможных

случаях, особенно при сохраняющейся активности заболевания на фоне терапии аналогами соматостатина

в максимальных дозировках. Доля пациентов с неконтролируемой акромегалией, «потерянных» их наблюдения, составила 18%, что сопоставимо с данными зарубежных исследований. Необходимо проведение

дополнительных исследований для оценки регулярности визитов пациентов с акромегалией к врачам

специализированных центров областного и федерального уровня, а также обеспечение преемственности

в ведении пациентов для возможности оценки отдаленных результатов лечения и оценки смертности.
КЛИНИЧЕСКИЕ ПРОЯВЛЕНИЯ, ДИАГНОСТИКА И ЭФФЕКТИВНОСТЬ ЛЕЧЕНИЯ БОЛЕЗНИ ИЦЕНКО-КУШИНГА В ПРИМОРСКОМ КРАЕ
Authors: Морозова А. М. , Цыганкова О. Г. , Резниченко А. В. , Виловатая Е. А.
Published: July 2023

ЦЕЛЬ: изучить клинические проявления и эффективность лечения у пациентов с болезнью ИценкоКушинга (БИК) в Приморском крае.

МАТЕРИАЛЫ И МЕТОДЫ: проведен ретроспективный анализ данных историй болезни 17 пациентов

с БИК, находившихся на лечении в Приморском Краевом Центре Диабета и Эндокринных Заболеваний

с 2016 г. по 2022 г.

РЕЗУЛЬТАТЫ: среди обследованных пациентов 88% - женщины, 12% - мужчины.

Средний возраст дебюта заболевания 39,1±5,8 лет. Клинические проявления заболевания: средний ИМТ

составил 33,9±4,7 кг/м2, артериальная гипертензия наблюдалась у 88% больных, нарушение углеводного

обмена – 76,5%, остеопороз – 41,2%. При проведение лучевой диагностики в 80% случаев выявлена микроаденома гипофиза, 20% - макроаденома гипофиза. Пациентам проводилась трансназальная транссфеноидальная аденомэктомия с нейрофизиологическим мониторингом. В 17,6% случаев отмечалась неполная

ремиссия и в 13,3% случае проведено повторное оперативное лечение. У одной пациентки повторное

оперативное нейрохирургическое лечение проведено через 5 лет при развитии клинико-гормонального

рецидива БИК без визуализации остаточной ткани аденомы с подтверждением гиперсекреции АКТГ при

селективном заборе из каменистых синусов. В послеоперационном периоде у 26,7% больных развивалась

вторичная надпочечниковая недостаточность, у 6,7% больных транзиторный несахарный диабет.

Медикаментозная терапия: При отсутствии ремиссии после трансназальной аденомэктомии

в 35,3% случаев назначали ингибиторы стероидогенеза и агонисты дофамина, в 11,8% - аналоги соматостатина.

Данные лабораторного обследования: Среднее значение АКТГ до операции – 131,1±11,2 пг/мл, после

операции - 19,36±3,9 пг/мл. Среднее значение кортизола в МДП до операции – 556,4±39,2 нмоль/л, после

операции 166,07±23,1 пг/мл. Показатели кортизола слюны в вечернее время до операции 17,2±3,8 нмоль/л.

после операции 5,7±1,2 нмоль/л.

По данным иммуногистохимии в 100% случаев наблюдалась сильная экспрессия АКТГ, в 90% случаев –

также наблюдалась сильная экспрессия СТГ, в 50% случаев сильная экспрессия пролактина. У большинства

(в 94% случаев) коэффициент пролиферации Ki67 составил 1-2%, в 6% случаев более 5%. Причем у одной

пациентки при первичной операции отмечался высокий Ki67 (7-10%), а при развитии рецидива при повторной операции отмечалось снижение уровня Ki67 до 1-2%.

ВЫВОДЫ: среди пациентов БИК в Приморском крае преобладает женская популяция, у большинства

пациентов выявлено ожирение легкой степени, среди клинических симптомов преобладают симптомы

артериальной гипертензии, нарушения углеводного обмена. Большинство опухолей – микроаденомы, обладающие смешанной гормональной активностью (АКТГ, СТГ, ПРЛ) с низкой пролиферацией. Современные

технологии нейрохирургического лечения. адекватный гормональный мониторинг позволяют предотвращать тяжелые осложнения БИК.