bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vivo Imaging of Cardiac Attachment of TcI and TcII Variants of Trypanosoma cruzi in a Zebrafish Model
Authors: Rodriguez-Castellanos Victoria E. , Perdomo-Gómez Cristhian David , Santos-Barbosa Juan Carlos , Forero-Shelton Manu , Akle Verónica , González John M.
Published: January 2025

Trypanosoma cruzi, the etiological agent of Chagas disease, is a parasite known for its diverse genotypic variants, or Discrete Typing Units (DTUs), which have been associated with varying degrees of tissue involvement. However, aspects such as parasite attachment remain unclear. It has been suggested that the TcI genotype is associated with cardiac infection, the most common involved site in chronic human infection, while TcII is associated with digestive tract involvement. Traditional models for T. cruzi infection provide limited in vivo observation, making it challenging to observe the dynamics of parasite-host interactions. This study evaluates the cardiac attachment of trypomastigotes from TcI and TcII DTUs in zebrafish larvae. Labeled trypomastigotes were injected in the duct of Cuvier of zebrafish larvae and tracked by stereomicroscopy and light-sheet fluorescence microscopy (LSFM). Remarkably, it was possible to observe TcI parasites adhered to the atrium, atrioventricular valve, and circulatory system, while TcII trypomastigotes demonstrated adhesion to the atrium, atrioventricular valve, and yolk sac extension. When TcI and TcII were simultaneously injected, they both attached to the heart; however, more of the TcII trypomastigotes were observed attached to this organ. Although TcII DTU has previously been associated with digestive tissue infection, both parasite variants showed cardiac tissue attachment in this in vivo model.
Peptides as Versatile Regulators in Cancer Immunotherapy: Recent Advances, Challenges, and Future Prospects
Authors: Lei Yu , Liu Jiacheng , Bai Yaowei , Zheng Chuansheng , Wang Dongyuan
Published: January 2025

The emergence of effective immunotherapies has revolutionized therapies for many types of cancer. However, current immunotherapy has limited efficacy in certain patient populations and displays therapeutic resistance after a period of treatment. To address these challenges, a growing number of immunotherapy drugs have been investigated in clinical and preclinical applications. The diverse functionality of peptides has made them attractive as a therapeutic modality, and the global market for peptide-based therapeutics is witnessing significant growth. Peptides can act as immunotherapeutic agents for the treatment of many malignant cancers. However, a systematic understanding of the interactions between different peptides and the host’s immune system remains unclear. This review describes in detail the roles of peptides in regulating the function of the immune system for cancer immunotherapy. Initially, we systematically elaborate on the relevant mechanisms of cancer immunotherapy. Subsequently, we categorize peptide-based nanomaterials into the following three categories: peptide-based vaccines, anti-cancer peptides, and peptide-based delivery systems. We carefully analyzed the roles of these peptides in overcoming the current barriers in immunotherapy, including multiple strategies to enhance the immunogenicity of peptide vaccines, the synergistic effect of anti-cancer peptides in combination with other immune agents, and peptide assemblies functioning as immune stimulators or vehicles to deliver immune agents. Furthermore, we introduce the current status of peptide-based immunotherapy in clinical applications and discuss the weaknesses and future prospects of peptide-based materials for cancer immunotherapy. Overall, this review aims to enhance comprehension of the potential applications of peptide-based materials in cancer immunotherapy and lay the groundwork for future research and clinical applications.
Spectroscopic Characterization and Biological Effects of 1-Oxo-bisabolone-rich Pulicaria burchardii Hutch. subsp. burchardii Essential Oil Against Viruses, Bacteria, and Spore Germination
Authors: Castagliuolo Giusy , Dell’Annunziata Federica , Pio Sara , Di Napoli Michela , Troiano Alessia , Antonini Dario , Badalamenti Natale , Bruno Maurizio , Ilardi Vincenzo , Folliero Veronica , Varcamonti Mario , Franci Gianluigi , Zanfardino Anna
Published: January 2025

Pulicaria species are used as herbal medicine and in the preparation of decoctions in several Asian and African regions. Among them, the plant Pulicaria burchardii is known for its medicinal properties, but comprehensive studies on its biological activity are still limited. This study examined the properties of the essential oil (EO) extracted by P. burchiardii and collected in Morocco during the flowering period. The focus was on its antimicrobial, anti-germinative, antioxidant, and antiviral activities, with the aim of evaluating its potential use in food preservation and beyond. The EO was subjected to various analyses to determine its chemical composition and biological efficacy. Specifically, GCMS and NMR analyses revealed that the EO is rich in oxygenated sesquiterpenes (72.59%), with 1-oxo-bisabolone being the predominant component (65.09%). The antimicrobial activity was tested against various Gram-positive and Gram-negative bacteria, demonstrating a significant inhibition of bacterial growth, particularly against Bacillus subtilis (MIC value of 0.6 mg/mL). The anti-germinative property was evaluated on spores of B. subtilis and other bacilli, such as Bacillus cereus, revealing a notable ability to prevent germination. For antiviral activity, the EO was tested against several pathogenic viruses including SARS-CoV-2 and HSV-1, showing an effective broad-spectrum reduction in viral replication in vitro. This study demonstrated that P. burchardii essential oil had excellent antibacterial and antiviral capabilities. The future challenge will focus mainly on the principal compound, 1-oxo-bisabolone, to demonstrate its real effectiveness as an antibacterial and/or antiviral.
Pyroxsulam Resistance in Apera spica-venti: An Emerging Challenge in Crop Protection
Authors: Bhattacharya Soham , Sen Madhab Kumar , Hamouzová Katerina , Košnarová Pavlína , Bharati Rohit , Menendez Julio , Soukup Josef
Published: January 2025

Apera spica-venti, a prevalent weed in Czech winter wheat fields, has developed resistance to ALS-inhibiting herbicides due to their frequent use. This study reports a biotype of A. spica-venti resistant to pyroxsulam, with cross and multiple resistance to iodosulfuron, propoxycarbazone, pinoxaden, and chlortoluron. Dose–response experiments revealed high resistance of both R1 and R2 biotypes to pyroxsulam, with resistance factors (RF) of 6.69 and 141.65, respectively. Pre-treatment with malathion reduced RF by 2.40× and 1.25× in R1 and R2, indicating the potential involvement of cytochrome P450 (CytP450). NBD-Cl pre-treatment decreased RF only in R2, suggesting possible GST involvement. Gene analysis revealed no mutations (at previously reported sites) or overexpression in the acetolactate synthase (ALS) gene. However, a significant difference in ALS enzyme activity between resistant and susceptible biotypes points to target-site resistance mechanisms. Studies with 14C-labeled pyroxsulam showed that reduced absorption and translocation were not likely resistance mechanisms. In summary, herbicide resistance in A. spica-venti appears to result from multiple mechanisms. Possible causes include target-site resistance from an unidentified ALS mutation (within coding or regulatory regions). Enhanced herbicide metabolism via CytP450s and GSTs is also a contributing factor. Further experimental validation is needed to confirm these mechanisms and fully understand the resistance. This evolution underscores the adaptive capacity of weed populations under herbicide pressure, emphasizing the need for alternative control strategies.
Brassinosteroid Enhances Cucumber Stress Tolerance to NaHCO3 by Modulating Nitrogen Metabolism, Ionic Balance and Phytohormonal Response
Authors: Nie Wenjing , Gong Biao , Wen Dan , Qiao Peng , Guo Hongen , Shi Qinghua
Published: January 2025

Under NaHCO3 stress, exogenous 24-epibrassinolide (EBR) markedly alleviated Na+ accumulation in cucumber plants, thereby decreasing the Na+/K+, Na+/Mg2+, and Na+/Ca2+ ratios. This mitigation was accompanied by elevated concentrations of K+, Ca2+, and Mg2+, as well as enhanced expression of the NHX and SOS1 genes. In addition, the activities of plasma membrane H+-ATPase, vesicular membrane H+-ATPase, and vesicular membrane H+-PPase were significantly increased, contributing to the maintenance of ionic balance in cucumber plants. NaHCO3 stress disrupted nitrogen metabolism, as evidenced by reductions in the activities of NR, GS, GOGAT, GOT, and GPT, along with altered GDH activity. These disruptions led to an accumulation of NH4+ and substantial decreases in NO3−-N and total nitrogen content. Exogenous EBR alleviated these effects by enhancing the activities of NR, GS, GOGAT, GOT, and GPT, countering the prolonged suppression of GDH activity, and restoring NO3−-N and total nitrogen levels. Consequently, EBR application reduced NH4+ toxicity induced by alkali stress. Additionally, NaHCO3 stress increased ABA accumulation while decreasing IAA and GA3 content in cucumber seedlings. In contrast, exogenous EBR application elevated IAA and GA3 levels and increased the IAA/ABA and GA3/ABA ratios, thus maintaining hormonal equilibrium under alkali stress. Collectively, these findings highlight that exogenous EBR enhances the alkaline tolerance of cucumber plants by regulating nitrogen metabolism, ion homeostasis, and phytohormonal responses.
Seasonal Variation in Chemical Composition and Antioxidant and Antibacterial Activity of Essential Oil from Cinnamomum cassia Leaves
Authors: Cheng Yanrong , Fu Ying , Gu Dingze , Huang Yan , Lu Yongqi , Liu Yujie , Li Xiulan , Yao Xinyue , Zhang Xinxuan , Jian Wanying , Liu Peiwei , Wu Hong , Li Yanqun
Published: January 2025

Cinnamomum cassia has been extensively utilized in traditional medicine systems worldwide. The essential oil (EO) content and composition are influenced by various external and internal factors, such as climate and harvest season, making it vital to determine the optimal harvest period for high-quality EO production. This study is the first to evaluate the chemical profiles, as well as the antioxidant and antibacterial activities, of C. cassia leaf oil across the four seasons. GC–MS and FTIR analyses revealed significant seasonal variations in the components. Spring and autumn leaf oils contained the highest EO (2.20% and 1.95%, respectively) and trans-cinnamaldehyde (92.59% and 91.10%, respectively). Temperature and humidity primarily affected EO and trans-cinnamaldehyde accumulation. C. cassia leaf oil demonstrated the strongest antibacterial activity, with a minimum inhibitory concentration (MIC) of 0.25 mg/mL against S. aureus and L. monocytogenes for the spring oil. The MICs for the other three seasonal samples were 0.5 mg/mL for S. aureus, M. luteus, and L. monocytogenes, and 1.0 mg/mL for P. putida. The minimum bactericidal concentration (MBC) of the EOs across all seasons against S. aureus ranged from 0.5 to 1 mg/mL. Winter leaf oil exhibited high antioxidant activity, primarily due to the presence of cis-cinnamaldehyde, caryophyllene, humulene, alloaromadendrene, γ-muurolene, cis-bisabolene, o-methoxycinnamaldehyde, and phenolics. This study provides essential data and valuable references for optimizing resource utilization and determining the ideal harvest time for C. cassia leaves.
Comparative Analysis of Mechanistic and Correlative Models for Global and Bhutan-Specific Suitability of Parthenium Weed and Vulnerability of Agriculture in Bhutan
Authors: Dorji Sangay , Stewart Stephen , Shabbir Asad , Bajwa Ali , Aziz Ammar , Adkins Steve
Published: January 2025

Parthenium weed (Parthenium hysterophorus L.) is one of the most noxious and fast-spreading invasive alien species, posing a major threat to ecosystems, agriculture, and public health worldwide. Mechanistic and correlative species distribution models are commonly employed to determine the potential habitat suitability of parthenium weed. However, a comparative analysis of these two approaches for parthenium weed is lacking, leaving a gap in understanding their relative effectiveness and ability to describe habitat suitability of parthenium weed. This study compared the mechanistic model CLIMEX with random forest (RF), the best-performing of a suite of correlative models. When compared against occurrence records and pseudo-absences, measured by area under the receiver operating characteristic curve, true skill statistic, sensitivity, and specificity, the results revealed higher performance of RF compared to CLIMEX. Globally, RF predicted 7 million km2 (2% of the total land mass) as suitable for parthenium weed, while CLIMEX predicted 20 million km2 (13%). Based on binary maps, RF and CLIMEX identified 67 and 20 countries as suitable, respectively. For Bhutan, globally trained RF predicted 8919 km2 (23% of the country’s total 38,394 km2) as currently suitable, with high suitability in the southern, west–central, central, and eastern districts, particularly along major highways. For the future, the 10 general circulation models downscaled to Bhutan showed a decrease in suitability across four scenarios (SSP126, SSP245, SSP370, SSP585) and three periods (2021–2050, 2051–2080, 2071–2100), with a northward shift in suitable habitats ranging from 2 to 76 km. Additionally, 2049 (23%) km2 of agricultural land is currently at risk of being invaded by parthenium weed. Correlative and mechanistic models are based on different niche concepts (i.e., realized and fundamental, respectively), and therefore combining them can provide a better understanding of actual and potential species distributions. Given the high suitability of parthenium weed under the current climate and its potential negative impacts in Bhutan, early action such as early detection and control of infested areas, regular survey and monitoring, and creating public awareness are proposed as risk mitigation strategies.
Pseudomonas syringae Pathovar syringae Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
Authors: Carreras Claudia , Zamorano Alan , Villalobos-González Luis , Pimentel Paula , Pizarro Lorena , Beltrán María Francisca , Cui Weier , Pinto Manuel , Figueroa Franco , Rubilar-Hernández Carlos , Llanes Analia , Bertaccini Assunta , Fiore Nicola
Published: January 2025

Pseudomonas syringae pv. syringae is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to P. syringae pv. syringae, respectively) after 1 and 7 days post-inoculation (dpi) with the bacterium. Symptoms of bacterial canker became evident from the fifth day. At 1 dpi, cultivar Santina showed a faster response to infection and a larger number of differentially expressed genes (DEGs) than cultivar Bing. At 7 dpi, cultivar Bing almost doubled its DEGs, while cultivar Santina tended to the normal DEG levels. P. syringae pv. syringae infection downregulated the expressions of key genes of the photosynthesis process at 1 dpi in the less susceptible cultivar. The results suggest that the difference in susceptibility to P. syringae pv. syringae is linked to the timeliness of pathogen recognition, limiting the bacteria’s dispersion through modeling its cell wall, and regulation of genes encoding photosynthesis pathway. Through this study, it has been possible to progress the knowledge of relevant factors related to the susceptibility of the two studied cherry cultivars to P. syringae pv. syringae.
Optical Methods for Determining the Phagocytic Activity Profile of CD206-Positive Macrophages Extracted from Bronchoalveolar Lavage by Specific Mannosylated Polymeric Ligands
Authors: Zlotnikov Igor D. , Ezhov Alexander A. , Kolganova Natalia I. , Ovsyannikov Dmitry Yurievich , Belogurova Natalya G. , Kudryashova Elena V.
Published: January 2025

Macrophage (Mph) polarization and functional activity play an important role in the development of inflammatory lung conditions. The previously widely used bimodal classification of Mph into M1 and M2 does not adequately reflect the full range of changes in polarization and functional diversity observed in Mph in response to various stimuli and disease states. Here, we have developed a model for the direct assessment of Mph from bronchial alveolar lavage fluid (BALF) functional alterations, in terms of phagocytosis activity, depending on external stimuli, such as exposure to a range of bacteria (E. coli, B. subtilis and L. fermentum). We have employed polymeric mannosylated ligands (the “trapping ligand”) specifically targeting the CD206 receptor to selectively isolate activated Mph from the BALF of patients with pulmonary inflammatory conditions: primary ciliary dyskinesia (PCD), pneumonia and bronchial asthma. An “imaging ligand” allows for the subsequent visualization of the isolated cells using a sandwich technique. Five model strains of E. coli, MH-1, JM109, BL21, W3110 and ATCC25922, as well as B. subtilis and L. fermentum strains, each exhibiting distinct properties and expressing red fluorescent protein (RFP), were used as a phagocytosis substrate. Fluorometric, FTIR- and confocal laser scanning microscopy (CLSM) assessments of the phagocytic response of Mph to these bacterial cells were performed. Mph absorbed different strains of E. coli with different activities due to the difference in the surface villosity of bacterial cells (pili and fimbriae, as well as signal patterns). In the presence of other competitor cells (like those of Lactobacilli), the phagocytic activity of Mph is changed between two and five times and strongly dependent on the bacterial strain. The relative phagocytic activity indexes obtained for BALF-Mph in comparison with that obtained for model human CD206+ Mph in the M1 polarization state (derived from THP-1 monocyte cultures) were considered as a set of parameters to define the Mph polarization profile from the BALF of patients. Mannan as a marker determining the selectivity of the binding to the CD 206 mannose receptor of Mph significantly inhibited the phagocytosis of E. coli and B. subtilis in cases of pneumonia, suggesting an important role of CD206 overexpression in acute inflammation. Conversely, L. fermentum binding was enhanced in PCD, possibly reflecting altered macrophage responsiveness in chronic lung diseases. Our approach based on the profiling of Mph from patient BALF samples in terms of phagocytosis for a range of model bacterial strains is important for the subsequent detailed study of the factors determining dangerous conditions and resistance to existing therapeutic options.
Improving the Electrochemical and Electrochromic Properties of Copolymerized 3,4-Ethylenedioxythiophene with Pyrene
Authors: Wang Xiang , Jiang Haiyun , Gan Muling , Zhang Jun , Wu Ruomei , Zhang Weili , Wang Ziyi , Guo Minxi , Mu Yangfan
Published: January 2025

Pyrene (Pr) was used to improve the electrochemical and electrochromic properties of polythiophene copolymerized with 3,4-ethylenedioxythiophene (EDOT). The corresponding product, poly(3,4-ethylenedioxythiophene-co-Pyrene) (P(EDOT-co-Pr)), was successfully synthesized by electrochemical polymerization with different monomer concentrations in propylene carbonate solution containing 0.1 M lithium perchlorate (LiClO4/PC (0.1 M)). The homopolymer and copolymer films were analyzed by Fourier transform infrared spectroscopy (FT-IR), color-coordinate and colorimetric methods, cyclic voltammetry (CV), spectroelectrochemistry (SEC), and UV–visible spectroscopy (UV-Vis). Homopolymer poly(3,4-ethylenedioxythiophene) (PEDOT) and the P(EDOT-co-Pr) copolymer were investigated, which included examining their colorimetric, electrochemical, and electrochromic characteristics. The color shifts resulting from redox reactions of the polymers were also observed. The copolymers with different monomer concentrations achieved multicolor shifts, such as light purple, dark blue, dark red, green, and earthy yellow. Moreover, P(EDOT-co-Pr) had a small optical bandgap (1.74–1.83 eV), excellent optical contrast (31.68–45.96%), and high coloring efficiency (350–507 cm2 C−1). In particular, P(EDOT1-co-Pr3) exhibited outstanding cycling stability, retaining 91% of its initial optical contrast after cycling for 10,000 s, and it is expected to be a promising candidate copolymer for electrochromic applications.
Utilizing Crushed Recycled Marble Stone Powder as a Sustainable Filler in SBS-Modified Asphalt Containing Recycled Tire Rubber
Authors: Ohm Byungsik , Lee Sang Yum , Le Tri Ho Minh
Published: January 2025

The increasing demand for sustainable construction materials has driven the exploration of alternative fillers in asphalt production. Traditional asphalt mixtures rely heavily on natural aggregates and petroleum-based binders, contributing to environmental degradation. This study proposes an innovative solution by utilizing Crushed Recycled Marble Stone Powder (CRMSP) as a sustainable filler in SBS polymer-modified asphalt containing high volumes of recycled tire rubber, addressing both resource depletion and waste management concerns. A total of 10 asphalt mixes were formulated with varying CRMSP content (0–100% as a replacement for conventional filler) and SBS polymer (3–5%), and their performance was evaluated through Marshall stability, flow, volumetric properties, and dynamic modulus tests. The results demonstrate that incorporating CRMSP up to 75% significantly enhances asphalt’s mechanical properties. The 75% CRMSP mix showed superior stability (19.2 kN, 24.1% improvement), flow (4.6 mm, 4.5% improvement), and resistance to rutting (lowest rut depth: 0.18 mm, 16.7% reduction) compared to the control mixture. Dynamic modulus testing further confirmed the improved resistance to deformation, with the 75% CRMSP mix exhibiting the highest modulus (6.9 GPa, 15.0% improvement). This research highlights the potential of CRMSP as an innovative and eco-friendly alternative filler, improving asphalt performance while reducing environmental impact. By offering a sustainable way to recycle marble waste and tire rubber, this study paves the way for greener, cost-effective asphalt formulations. Future studies should focus on real-world applications, durability, and long-term performance to validate the potential of CRMSP-modified asphalt in commercial use.
Effect of Extensive Solar Ultra-Violet Irradiation on the Durability of High-Density Polyethylene- and Polypropylene-Based Wood–Plastic Composites
Authors: Siddiqui Mohammad N. , Redhwi Halim H. , Andrady Anthony L. , Furquan Sarfaraz A. , Hussain Syed
Published: January 2025

The natural and laboratory-accelerated weathering of wood–plastic composites (WPCs) based on high-density polyethylene (HDPE) and polypropylene (PP) plastics was investigated in this study. Injection molded samples of WPCs with different loadings of wood fiber ranging from 0 to 36 wt.% of wood were subjected to laboratory-accelerated weathering and natural weathering. The integrity of samples weathered to different extents was tested using a standard tensile test and surface hardness test to investigate the dependence of these properties on the duration of weathering exposure. Tensile data were used to identify the loading of wood fibers in either plastic matrix that afforded superior ultra-violet (UV) stability. Tensile measurements under uniaxial strain yielded average values of tensile strength (TS), low-extension modulus (E), and elongation at break (EB). Both natural weathering outdoors and accelerated weathering in the laboratory showed that the TS and EB decreased while the E increased with the duration of exposure for all samples tested. The change in the average TS of composites with the duration of exposure offers valuable insights. The correlation between the tensile and hardness data for the WPC samples was explored. After naturally weathering at two exposure sites, the hardness of the WPCs was found to decrease between 8% to 12.5%, depending on the composition and exposure location parameters. Furthermore, no marked difference in performance with increasing wood fiber beyond 18 wt.% was observed. WPCs can be a key parameter in environmental sustainability by being used in the building and packaging industries, which reduces carbon emissions and waste generation.
Fluorescent Polymers via Coordination of bis-Terpyridine Ligands with Transition Metals and Their pH Response Properties
Authors: Zhang Tao , Liu Fengxue , Liu Yongxin , Li Kaixiu , Li Zhengguang , Li Yaqin , Fu Fan , Liu Mingliang , Li Yiming , Liu Die , Wang Pingshan
Published: January 2025

Stimulus-responsive luminescent materials are pivotal in the field of sensing. Fluorescent transition metal complexes with a charge transfer excited state, especially terpyridine-coordinated polymers, are of particular interest due to their tunable emission. In this paper, a novel bis-terpyridine ligand was synthesized and assembled into a coordination polymer, which showed intense visible light absorption and fluorescence emission in the solid state that could be regulated by an acidic or basic pH. After being protonated by acid, the fluorescence of the polymer P2 was quenched. The emission of the polymer split from 635 nm to two peaks of 674 and 440 nm, and then stabilized at 728 nm for 7 days, which showed a significant red-shift and good protonation stability. The fluorescence emission wavelength of the protonated polymers recovered after alkalization, and the fluorescence intensity of the polymer was greatly improved after alkalization, showing interesting acid–base-response luminescence characteristics. The sensitive response of the synthesized coordination polymers to acids and bases will contribute to expanding the application of linear coordination polymers in sensing and other fields.
Synthesis of Highly Expandable Poly(methacrylimide) (PMI) Precursor Beads Through Optimized Suspension Polymerization of MAA-MAN-tBMA Copolymers
Authors: Wang Haozhe , Gao Yusong , Yin Zhiying , Qin Jianbin , Zhao Yongsheng , Zhang Guangcheng
Published: January 2025

Bead-foaming technology effectively addresses production cycles, polymerization control, and cellular structure defects in conventional bulk foaming, especially in high-performance PMI foams. In this work, highly expandable PMI beads were synthesized based on the aqueous suspension polymerization of methacrylic acid-methacrylonitrile-tert-butyl methacrylate (MAA-MAN-tBMA) copolymers. The suspension polymerization was stabilized by reducing the solubility of MAA by the salting-out effect and replacing formamide (a common PMI foaming agent) with tBMA. The polymerization process was optimized by varying salting-out agents, dispersants, water-to-oil ratio (WOR), and stirring speed to achieve uniform bead sizes (0.2–0.4 mm) and high bead yields (>70%). The expansion ratio of the beads can be easily tuned by adjusting tBMA content and foaming time and temperature. Beads with 10%tBMA can reach up to 64 times under a free-forming process at 240 °C, which serves as an excellent precursor toward high-performance in-mold foaming PMI. The beads exhibit excellent in-mold foaming capabilities, thermal stability (Td = 392 °C), and mechanical properties. This work provides a technical foundation for the bead-foaming technology of PMI foams, reducing the cost of PMI foam production and providing the possibility to expand the application of PMI foam in civilian use.
Design and Application of Wireless Wall Thickness Monitoring System for Ground Testing Process
Authors: He Yufa , Chen Yu , Wei Jianfei , Li Zhong , Guo Xingwang , Xie Renjun , Li Ruiling , Liu Jian , Tan Zhenxing , Zhang Kexin
Published: January 2025

To address the issues of pipeline corrosion and erosion during ground testing, this paper presents an innovative electromagnetic ultrasonic thickness measurement system that utilizes ZigBee wireless communication technology. The system employs a ZigBee mesh topology for creating a wireless distributed network, where node devices carry out multi-point monitoring in a configuration of “one master, multiple”. Each node is powered by an STM32 embedded control chip and fitted with ultrasonic sensors. Slave nodes transmit the real-time data they collect to a server via the master node, thus enabling remote monitoring of the system through a web interface. The system incorporates an enhanced data filtering algorithm, allowing for precise monitoring of the pipeline wall thickness and providing immediate data feedback. An experimental validation of the system’s stability and long-distance transmission capabilities was performed on a simulated platform, confirming its viability and applicability for real-world engineering applications.
The Behavior of Fluid Flow and Solute Transport in 3D Crossed Rock Fractures
Authors: Han Xuefeng , Xue Kangsheng , Zhang Shaojie
Published: January 2025

Understanding the behavior of fluid flow and solute transport in fractured rock is of great significance to geoscience and engineering. The discrete fracture network is the predominate channel for fluid flow through fractured rock as the permeability of fracture is several magnitudes higher than that of the rock matrix. As the basic components of the fracture network, investigating the fluid flow in crossed fractures is the prerequisite of understanding the fluid flow in fractured rock. First, a program based on the successive random addition algorithm was developed to generate rough fracture surfaces. Next, a series of fracture models considering shear effects and different surface roughness were constructed. Finally, fluid dynamic analyses were performed to understand the role of flowrate and surface roughness in the evolution of flow field, concentration field, solute breakthrough, and solute mixing inside the crossed fractures. Results indicated that the channeling flow at the fracture intersection became more pronounced with the increasing Péclet number (Pe) and Joint Roughness Coefficient (JRC), the evolution of the concentration field was influenced by Pe and the distribution of the concentration field was influenced by JRC. For Pe < 10, the solute transport process was dominated by molecular diffusion. For 100 > Pe > 10, the solute transport process was in the complete mixing mode. In addition, for Pe > 100, the solute transport process was in the streamline routing mode. The concentration distribution was affected by the local aperture at the fracture intersection corresponding to different surface roughness. Meanwhile, the solute mixing equation was improved based on this result. The research results are beneficial for further revealing the mechanism of fluid flow and solute transport phenomenon in fractured rock.
Bandgap-Tunable Aluminum Gallium Oxide Deep-UV Photodetector Prepared by RF Sputter and Thermal Interdiffusion Alloying Method
Authors: Liao Che-Hao , Huang Jing-Yun , Huang Chien-Sheng , Yang Chih-Chiang , Kuo Jui-En , Water Walter , Tsai Wan-Shao , Miranda Cortez Patsy A , Tang Xiao , Lin Shih-Hung
Published: January 2025

Gallium oxide (Ga2O3) has gained considerable attention due to its wide bandgap, the availability of native substrates, and its excellent properties for solar-blind photodetectors, transparent electronics, and next-generation power devices. However, the expensive Ga2O3 native substrates have restricted its widespread adoption. To reduce costs and further the development of β-Ga2O3-based devices, there is a need for bandgap-tunable oxide films with high crystal quality for deep-ultraviolet (DUV) photodetectors and high-breakdown-field power devices. This study introduces a Thermal Interdiffusion Alloying method to address these requirements. It focuses on developing deep ultraviolet (DUV) photodetectors using β-Ga2O3 thin films on sapphire substrates by promoting the diffusion of aluminum (Al) atoms from the substrate into the film, resulting in the formation of aluminum gallium oxide (β-(AlxGa1−x)2O3). The aluminum content is controlled by adjusting the process temperature, allowing for tunable detection wavelengths and enhanced DUV sensing capabilities. Radio frequency (RF) sputtering optimizes the film’s quality by adjusting the sputtering power and the argon/oxygen (Ar/O2) flow ratio. Material analysis indicates that this method expands the optical bandgap and shifts the response wavelength to 210 nm, significantly boosting the performance of the fabricated photodetectors. This research presents considerable potential for advancing DUV photodetectors across various disinfection applications.
Artificial Intelligence of Things (AIoT) Advances in Aquaculture: A Review
Authors: Huang Yo-Ping , Khabusi Simon Peter
Published: January 2025

The integration of artificial intelligence (AI) and the internet of things (IoT), known as artificial intelligence of things (AIoT), is driving significant advancements in the aquaculture industry, offering solutions to longstanding challenges related to operational efficiency, sustainability, and productivity. This review explores the latest research studies in AIoT within the aquaculture industry, focusing on real-time environmental monitoring, data-driven decision-making, and automation. IoT sensors deployed across aquaculture systems continuously track critical parameters such as temperature, pH, dissolved oxygen, salinity, and fish behavior. AI algorithms process these data streams to provide predictive insights into water quality management, disease detection, species identification, biomass estimation, and optimized feeding strategies, among others. Much as AIoT adoption in aquaculture is advantageous on various fronts, there are still numerous challenges, including high implementation costs, data privacy concerns, and the need for scalable and adaptable AI models across diverse aquaculture environments. This review also highlights future directions for AIoT in aquaculture, emphasizing the potential for hybrid AI models, improved scalability for large-scale operations, and sustainable resource management.
Mapping the Knowledge Domain of Pressure Vessels and Piping Fields for Safety Research in Industrial Processes: A Bibliometric Analysis
Authors: Mei Ting , Tong Chaozhen , Tong Bingrui , Zhu Junjie , Wang Yuxuan , Kou Mengyao , Liu Hui
Published: January 2025

With the rapid advancement of modern industries, pressure vessels and piping have become increasingly integral to sectors such as energy, petrochemicals, and process industries. To grasp the research and application status in the field of pressure vessel and piping safety, 670 publications in the Web of Science core database from 2008 to 2024 were taken as data samples in this paper. The knowledge mapping tools were used to carry out co-occurrence analysis, keyword burst detection, and co-citation analysis. The results show that the research in this field presents a multidisciplinary and cross-disciplinary state, involving multiple disciplines such as Nuclear Science and Technology, Engineering Mechanics, and Energy and Fuels. The “International Journal of Hydrogen Energy”, “International Journal of Pressure Vessels and Piping”, and “Nuclear Engineering and Design” are the primary publication outlets in this domain. The study identifies three major research hotspots: (1) the safety performance of pressure vessels and piping, (2) structural integrity, failure mechanisms, and stress analysis, and (3) numerical simulation and thermal–hydraulic analysis under various operating conditions. The current challenges can be summarized into three aspects: (1) addressing the safety risks brought by new technologies and materials, (2) promoting innovation and the application of detection and monitoring technologies, and (3) strengthening the building capacity for accident prevention and emergency management. Specific to China, the current challenges include the safety and management of aging equipment, the effective detection of circumferential weld cracks, the refinement of risk assessment models, and the advancement of smart technology applications. These findings offer valuable insights for advancing safety practices and guiding future research in this multidisciplinary field.
Process Improvement and Economic and Environmental Evaluation of Bio-Hydrogenated Diesel Production from Refined Bleached Deodorized Palm Oil
Authors: Anantpinijwatna Amata , Simasatitkul Lida , Yooyen Kanokporn , Amornraksa Suksun , Assabumrungrat Suttichai , Im-orb Karittha
Published: January 2025

The co-production of BHD with other renewable fuels (i.e., using a novel process involving carbon dioxide utilization to achieve the global sustainability goal) is presented. The three configurations of BHD production from refined bleached deodorized palm oil (RBDPO), including (1) the conventional BHD process with hydrogen recovery (BHD process), (2) the BHD process coupled with the Fischer–Tropsch process (BHD-FT process), and (3) the BHD process coupled with the bio-jet fuel and methanol processes (BHD-BIOJET-MEOH process) are investigated using the process model developed in Aspen Plus. The effect of the operating parameters is studied, and the condition of each process offering the highest BHD yield is proposed. Then, the pinch analysis and heat exchanger network (HEN) design of each proposed process are performed to find the highest energy-efficient configuration. The economic and environmental analysis is later performed to investigate the sustainability performance of each configuration. The conventional BHD process requires less hydrogen and consumes less energy than the others. The BHD-BIOJET-MEOH process is the most economically feasible, offering the highest net present value (NPV) of USD 7.93 million and the shortest payback period of 3 years and 1 month. However, it offers the highest carbon footprint of 0.820 kgCO2 eq./kg of BHD, and it presented the highest potential environmental impact (PEI) in all categories.
Advanced Insights into Methane Hydrate Production: Influence of Pressure, Saturation, and Permeability Dynamics
Authors: Zhou Yunjian , He Yufa , Chen Yu , Sun Shihui
Published: January 2025

The decomposition of hydrate during hydrate mining can reduce the strength of the formation and induce engineering geological disasters. Clarifying the decomposition characteristics of geological hydrate during hydrate mining is of great significance for preventing marine geological disasters. This study comprehensively examines the effects of various extraction conditions, including production pressure, hydrate saturation, and permeability, on methane hydrate decomposition during depressurization-based extraction. Key findings show that reduced production pressure significantly increases gas and water production rates due to an enhanced pressure differential, albeit at the cost of potential geomechanical instability. Variations in hydrate saturation reveal that lower-saturation reservoirs initially exhibit higher production due to faster pressure propagation and greater porosity, whereas high-saturation layers may sustain production in the later stages. Permeability changes impact pressure diffusion and heat transfer within the formation; higher permeability leads to faster initial production but causes rapid energy depletion, requiring supplementary energy inputs to maintain production. These findings provide essential insights for optimizing methane hydrate extraction, ensuring high productivity while mitigating formation stability risks.
Expanding Ethical Horizons: Rethinking the Ethics of De 德 and Guṇa in the Laozi 老子 and Bhagavad-Gītā
Authors: Saha Pritam
Published: January 2025

This paper aims to engage in an ethical discussion of de in the Laozi and guṇa in the Bhagavad-Gītā to expand the horizon of our ethical understanding of Chinese and Indian philosophy. First, this paper will explore the different ethical levels of de and guṇa and discuss how these levels operate and are bound together. From an ethical perspective, this paper points out that de and guṇa can each be divided into two parts—higher de and lower de, and higher guṇa and lower guṇa. It will further explain the ethical significance of de and guṇa, arguing that they offer a unique contribution to developing our contemporary understanding of ethics. We will also seek to discover the interplay of de and/with the dao in the Laozi and that among the three guṇas in the Gītā from the perspective of human behaviors. In the conclusion, we will provide a comparative discussion of our findings on the ethics of de and guṇa in the Laozi and the Gītā. These findings suggest that de and guṇa promote a shared philosophical outlook within their respective traditions and thereby make a contribution to post-comparative ethical approaches.
Influence of Physicochemical Properties of Oil Sludge on Syngas Production for Energy Applications
Authors: Oliveira Hiago , Pinheiro Isabela , Ramos Ana , Venturini Osvaldo , Mariano Adriano , Santiago York
Published: January 2025

Oil sludge (OS) is a hazardous waste generated in the refinery and platform production chain. Its recovery is globally limited by methods like incineration, landfilling, and stabilization, which are costly and environmentally harmful. In Brazil, advanced techniques such as gasification are still underdeveloped compared to established practices elsewhere. This study aims to characterize the chemical and physical properties of OS to enable its recovery through energy methods, reducing environmental impacts. OS samples from oil storage tanks were analyzed using mass spectrometry, thermogravimetry, atomic absorption, proximate analysis, X-ray fluorescence, and X-ray diffraction. The viscosity was approximately 34,793 cP, with 36.41% carbon and 56.80% oxygen. The ash content was 43.218% (w/w), and the lower and upper heating values were 17.496 and 19.044 MJ/kg, respectively. Metal analysis identified lead, vanadium, manganese, and chromium. The high ash content of OS reduced gasification temperatures, increasing char yield (44.6%). Increasing the equivalence ratio (ER) led to higher gasification temperatures, producing energetic species such as H2, CH4, and CO, raising the calorific value of the resulting syngas. Subsequently, this syngas was used in gas turbine models with GasTurb software 14.0, achieving electrical output and thermal efficiency of 66.9 kW and 22.4%, respectively. OS is a persistent waste requiring gasification treatment, offering a promising solution that converts these residues into valuable syngas for energy conversion with minimal environmental impact.
EOR: An Enhanced Object Registration Method for Visual Images and High-Definition Maps
Authors: Hui Nian , Jiang Zijie , Cai Zhongliang , Ying Shen
Published: January 2025

Accurate object registration is crucial for precise localization and environment sensing in autonomous driving systems. While real-time sensors such as cameras and radar capture the local environment, high-definition (HD) maps provide a global reference frame that enhances localization accuracy and robustness, especially in complex scenarios. In this paper, we propose an innovative method called enhanced object registration (EOR) to improve the accuracy and robustness of object registration between camera images and HD maps. Our research investigates the influence of spatial distribution factors and spatial structural characteristics of objects in visual perception and HD maps on registration accuracy and robustness. We specifically focus on understanding the varying importance of different object types and the constrained dimensions of pose estimation. These factors are integrated into a nonlinear optimization model and extended Kalman filter framework. Through comprehensive experimentation on the open-source Argoverse 2 dataset, the proposed EOR demonstrates the ability to maintain high registration accuracy in lateral and elevation dimensions, improve longitudinal accuracy, and increase the probability of successful registration. These findings contribute to a deeper understanding of the relationship between sensing data and scenario understanding in object registration for vehicle localization.
A Study of NOAA-20 VIIRS Band M1 (0.41 µm) Striping over Clear-Sky Ocean
Authors: Wang Wenhui , Cao Changyong , Blonski Slawomir , Shao Xi
Published: January 2025

The Visible Infrared Imaging Radiometer Suite (VIIRS) onboard the National Oceanic and Atmospheric Administration-20 (NOAA-20) satellite was launched on 18 November 2017. The on-orbit calibration of the NOAA-20 VIIRS visible and near-infrared (VisNIR) bands has been very stable over time. However, NOAA-20 operational M1 (a dual gain band with a center wavelength of 0.41 µm) sensor data records (SDR) have exhibited persistent scene-dependent striping over clear-sky ocean (high gain, low radiance) since the beginning of the mission, different from other VisNIR bands. This paper studies the root causes of the striping in the operational NOAA-20 M1 SDRs. Two potential factors were analyzed: (1) polarization effect-induced striping over clear-sky ocean and (2) imperfect on-orbit radiometric calibration-induced striping. NOAA-20 M1 is more sensitive to the polarized lights compared to other NOAA-20 short-wavelength bands and the similar bands on the Suomi NPP and NOAA-21 VIIRS, with detector and scan angle-dependent polarization sensitivity up to ~6.4%. The VIIRS M1 top of atmosphere radiance is dominated by Rayleigh scattering over clear-sky ocean and can be up to ~70% polarized. In this study, the impact of the polarization effect on M1 striping was investigated using radiative transfer simulation and a polarization correction method similar to that developed by the NOAA ocean color team. Our results indicate that the prelaunch-measured polarization sensitivity and the polarization correction method work well and can effectively reduce striping over clear-sky ocean scenes by up to ~2% at near nadir zones. Moreover, no significant change in NOAA-20 M1 polarization sensitivity was observed based on the data analyzed in this study. After the correction of the polarization effect, residual M1 striping over clear-sky ocean suggests that there exists half-angle mirror (HAM)-side and detector-dependent striping, which may be caused by on-orbit radiometric calibration errors. HAM-side and detector-dependent striping correction factors were analyzed using deep convective cloud (DCC) observations (low gain, high radiances) and verified over the homogeneous Libya-4 desert site (low gain, mid-level radiance); neither are significantly affected by the polarization effect. The imperfect on-orbit radiometric calibration-induced striping in the NOAA operational M1 SDR has been relatively stable over time. After the correction of the polarization effect, the DCC-based striping correction factors can further reduce striping over clear-sky ocean scenes by ~0.5%. The polarization correction method used in this study is only effective over clear-sky ocean scenes that are dominated by the Rayleigh scattering radiance. The DCC-based striping correction factors work well at all radiance levels; therefore, they can be deployed operationally to improve the quality of NOAA-20 M1 SDRs.