bluuebunny/crossref_metadata_2025 huggingface dataset:
Heparin-Mediated Extracorporeal Low-Density Lipoprotein Precipitation Apheresis for Treating Peripheral Arterial Disease in Patients with Chronic Kidney Disease
Authors: Rotella Stefania , Gesualdo Loreto , Fiorentino Marco
Published: February 2024

Patients with chronic kidney disease (CKD), particularly those with end-stage renal disease (ESRD), have a high prevalence of cardiovascular disease and peripheral arterial disease (PAD). Medical treatment is mainly based on risk factor management, and the surgical approach remains the gold standard treatment in specific conditions. Heparin-mediated extracorporeal low-density lipoprotein precipitation (H.E.L.P.) apheresis is effective in reducing circulating lipoprotein, fibrinogen, inflammatory mediators and procoagulant factors, thereby reducing cardiovascular risk in patients with familial hypercholesterolemia and hypertriglyceridemia. These activities may be effective in reducing symptoms and ischemic vascular lesions even in patients with severe PAD. We reported the application of a treatment protocol with H.E.L.P. apheresis in an ESRD patient with severe PAD without clinical improvement after severe revascularization who was not suitable for further surgical approaches, despite normal LDL cholesterol and lipoprotein (a). The H.E.L.P. protocol was characterized by an intensive first phase with weekly treatments followed by a single session every 10–15 days for 6 months of treatment. The overall clinical condition, foot lesions and walking distance improved significantly after the first 2 months of treatment, and foot amputation was avoided. Here, we review the main pathogenetic mechanisms through which LDL apheresis improves microcirculation and clinical outcomes. Its wider application may represent an optimal therapeutic option for patients unresponsive to standard treatment.
Surface Functionalization of Titanium-Based Implants with a Nanohydroxyapatite Layer and Its Impact on Osteoblasts: A Systematic Review
Authors: Homa Karolina , Zakrzewski Wojciech , Dobrzyński Wojciech , Piszko Paweł J. , Piszko Aleksandra , Matys Jacek , Wiglusz Rafal J. , Dobrzyński Maciej
Published: February 2024

This study aims to evaluate the influence of a nanohydroxyapatite layer applied to the surface of titanium or titanium alloy implants on the intricate process of osseointegration and its effect on osteoblast cell lines, compared to uncoated implants. Additionally, the investigation scrutinizes various modifications of the coating and their consequential effects on bone and cell line biocompatibility. On the specific date of November 2023, an exhaustive electronic search was conducted in esteemed databases such as PubMed, Web of Science, and Scopus, utilizing the meticulously chosen keywords ((titanium) AND ((osteoblasts) and hydroxyapatite)). Methodologically, the systematic review meticulously adhered to the PRISMA protocol. Initially, a total of 1739 studies underwent scrutiny, with the elimination of 741 duplicate records. A further 972 articles were excluded on account of their incongruence with the predefined subjects. The ultimate compilation embraced 26 studies, with a predominant focus on the effects of nanohydroxyapatite coating in isolation. However, a subset of nine papers delved into the nuanced realm of its modifiers, encompassing materials such as chitosan, collagen, silver particles, or gelatine. Across many of the selected studies, the application of nanohydroxyapatite coating exhibited a proclivity to enhance the osseointegration process. The modifications thereof showcased a positive influence on cell lines, manifesting in increased cellular spread or the attenuation of bacterial activity. In clinical applications, this augmentation potentially translates into heightened implant stability, thereby amplifying the overall procedural success rate. This, in turn, renders nanohydroxyapatite-coated implants a viable and potentially advantageous option in clinical scenarios where non-modified implants may not suffice.
Expanding the Toolbox for Genetic Manipulation in Pseudogymnoascus: RNAi-Mediated Silencing and CRISPR/Cas9-Mediated Disruption of a Polyketide Synthase Gene Involved in Red Pigment Production in P. verrucosus
Authors: Palma Diego , Oliva Vicente , Montanares Mariana , Gil-Durán Carlos , Travisany Dante , Chávez Renato , Vaca Inmaculada
Published: February 2024

Fungi belonging to the genus Pseudogymnoascus have garnered increasing attention in recent years. One of the members of the genus, P. destructans, has been identified as the causal agent of a severe bat disease. Simultaneously, the knowledge of Pseudogymnoascus species has expanded, in parallel with the increased availability of genome sequences. Moreover, Pseudogymnoascus exhibits great potential as a producer of specialized metabolites, displaying a diverse array of biological activities. Despite these significant advancements, the genetic landscape of Pseudogymnoascus remains largely unexplored due to the scarcity of suitable molecular tools for genetic manipulation. In this study, we successfully implemented RNAi-mediated gene silencing and CRISPR/Cas9-mediated disruption in Pseudogymnoascus, using an Antarctic strain of Pseudogymnoascus verrucosus as a model. Both methods were applied to target azpA, a gene involved in red pigment biosynthesis. Silencing of the azpA gene to levels of 90% or higher eliminated red pigment production, resulting in transformants exhibiting a white phenotype. On the other hand, the CRISPR/Cas9 system led to a high percentage (73%) of transformants with a one-nucleotide insertion, thereby inactivating azpA and abolishing red pigment production, resulting in a white phenotype. The successful application of RNAi-mediated gene silencing and CRISPR/Cas9-mediated disruption represents a significant advancement in Pseudogymnoascus research, opening avenues for comprehensive functional genetic investigations within this underexplored fungal genus.
Individualized Perioperative Hemodynamic Management Using Hypotension Prediction Index Software and the Dynamics of Troponin and NTproBNP Concentration Changes in Patients Undergoing Oncological Abdominal Surgery
Authors: Cylwik Jolanta , Celińska-Spodar Małgorzata , Dudzic Mariusz
Published: February 2024

Introduction: Abdominal oncologic surgeries pose significant risks due to the complexity of the surgery and patients’ often weakened health, multiple comorbidities, and increased perioperative hazards. Hypotension is a major risk factor for perioperative cardiovascular complications, necessitating individualized management in modern anesthesiology. Aim: This study aimed to determine the dynamics of changes in troponin and NTproBNP levels during the first two postoperative days in patients undergoing major cancer abdominal surgery with advanced hemodynamic monitoring including The AcumenTM Hypotension Prediction Index software (HPI) (Edwards Lifesciences, Irvine, CA, USA) and their association with the occurrence of postoperative cardiovascular complications. Methods: A prospective study was conducted, including 50 patients scheduled for abdominal cancer surgery who, due to the overall risk of perioperative complications (ASA class 3 or 4), were monitored using the HPI software. Hypotension was qualified as at least one ≥ 1 min episode of a MAP < 65 mm Hg. Preoperatively and 24 and 48 h after the procedure, the levels of NTproBNP and troponin were measured, and an ECG was performed. Results: We analyzed data from 46 patients and found that 82% experienced at least one episode of low blood pressure (MAP < 65 mmHg). However, the quality indices of hypotension were low, with a median time-weighted average MAP < 65 mmHg of 0.085 (0.03–0.19) mmHg and a median of 2 (2–1.17) minutes spent below MAP < 65 mmHg. Although the incidence of perioperative myocardial injury was 10%, there was no evidence to suggest a relationship with hypotension. Acute kidney injury was seen in 23.9% of patients, and it was significantly associated with a number of episodes of MAP < 50 mmHg. Levels of NTproBNP were significantly higher on the first postoperative day compared to preoperative values (285.8 [IQR: 679.8] vs. 183.9 [IQR: 428.1] pg/mL, p < 0.001). However, they decreased on the second day (276.65 [IQR: 609.4] pg/mL, p = 0.154). The dynamics of NTproBNP were similar for patients with and without heart failure, although those with heart failure had significantly higher preoperative concentrations (435.9 [IQR: 711.15] vs. 87 [IQR: 232.2] pg/mL, p < 0.001). Patients undergoing laparoscopic surgery showed a statistically significant increase in NTproBNP. Conclusions: This study suggests that advanced HPI monitoring in abdominal cancer surgery effectively minimizes intraoperative hypotension with no significant NTproBNP or troponin perioperative dynamics, irrespective of preoperative heart failure.
Impacts of Neoadjuvant Chemotherapy on Perioperative Outcomes in Patients with Bladder Cancer Treated with Radical Cystectomy: A Single High-Volume Center Experience
Authors: Proietti Flavia , Flammia Rocco Simone , Licari Leslie Claire , Bologna Eugenio , Bove Alfredo Maria , Brassetti Aldo , Tuderti Gabriele , Mastroianni Riccardo , Tufano Antonio , Simone Giuseppe , Leonardo Costantino
Published: February 2024

(1) Background: Less than 30% of patients with muscle-invasive bladder cancer (MIBC) receive neoadjuvant chemotherapy (NAC), and reasons for underuse remain unclear. One potential explanation is the concern for the increased risk of perioperative morbidity and mortality. The aim of this study is to investigate the impact of NAC on the risk of detrimental perioperative outcomes in patients with MIBC treated with radical cystectomy (RC). (2) Methods: We identified patients receiving RC for MIBC (T2-4a N0 M0) from 2016 to 2022. Moreover, 1:1 propensity score matching (PSM) was applied between RC alone versus RC plus NAC, and our analysis tested the association between NAC status and peri-operative outcomes. (3) Results: Among the 317 patients treated with RC for identified MIBC, 98 (31%) received NAC. Patients treated with NAC were younger (median yr. 64 vs. 71; p < 0.001), with a lower Charlson Comorbidity Index (3 vs. 4; p > 0.001), and received more frequently continent urinary diversion (61 vs. 32%, p < 0.001). About 43% of patients in each group were treated with robot-assisted radical cystectomy (RARC) with totally intracorporeal urinary diversion (ICUD). After PSM, no differences were detected for the outcomes considered. (4) Conclusions: NAC is not associated with a higher rate of perioperative complications, including patients who received RARC with ICUD.
Consumption of Sustainable Denim Products: The Contribution of Blockchain Certified Eco-Labels
Authors: Lou Xingqiu , Xu Yingjiao
Published: February 2024

Consumers’ growing interest in the environmental and social impacts of products has increased demand for sustainable fashion items, particularly denim. Emerging technologies such as blockchain technology and labeling certifications have been developed to address sustainability issues by improving supply chain transparency and efficiency. This research investigates the trade-offs consumers make when purchasing sustainable denim jeans and the impact of sociodemographic factors on their decision-making process. Employing a conjoint analysis approach, four attributes were examined: price, brand name, types of materials, and eco-labeling. The results indicated that price is still the most influential factor, followed by material, brand name, and eco-label. Although eco-labeling is of little importance to consumers, it offers valuable insights for effective communication of sustainable practices. Consumers prefer denim with a blockchain eco-label, followed by a fair-trade certificate. This research enhances the understanding of consumer behavior toward sustainable consumption and offers strategic insights for denim producers and marketers.
Functional Framework for Multivariant E-Commerce User Interfaces
Authors: Wasilewski Adam
Published: February 2024

Modern e-businesses heavily rely on advanced data analytics for product recommendations. However, there are still untapped opportunities to enhance user interfaces. Currently, online stores offer a single-page version to all customers, overlooking individual characteristics. This paper aims to identify the essential components and present a framework for enabling multiple e-commerce user interfaces. It also seeks to address challenges associated with personalized e-commerce user interfaces. The methodology includes detailing the framework for serving diverse e-commerce user interfaces and presenting pilot implementation results. Key components, particularly the role of algorithms in personalizing the user experience, are outlined. The results demonstrate promising outcomes for the implementation of the pilot solution, which caters to various e-commerce user interfaces. User characteristics support multivariant websites, with algorithms facilitating continuous learning. Newly proposed metrics effectively measure changes in user behavior resulting from different interface deployments. This paper underscores the central role of personalized e-commerce user interfaces in optimizing online store efficiency. The framework, supported by machine learning algorithms, showcases the feasibility and benefits of different page versions. The identified components, challenges, and proposed metrics contribute to a comprehensive solution and set the stage for further development of personalized e-commerce interfaces.
Has the Establishment of High-Tech Zones Improved Urban Economic Resilience? Evidence from Prefecture-Level Cities in China
Authors: Yu Ruoxi , Xia Xingneng , Huang Tao , Zhang Sheng , Zhou Wenguang
Published: February 2024

The establishment of high-tech zones in China represents a significant policy tool aimed at fostering urban scientific and technological innovation while ensuring steady and sustainable economic growth. Using high-tech zones as a quasi-natural experiment and 233 prefecture-level cities in China from 1990 to 2021 as a research sample, this article constructs a difference-in-difference model to test the impact of high-tech zones on urban economic resilience. Our findings reveal several key insights. First, high-tech zones play a crucial role in enhancing urban economic resilience, which is robust across multiple tests. Second, there is significant variation in the influence of high-tech zones on urban economic resilience. Large cities, cities in the Yangtze River Economic Belt (YEB), and eastern cities are more affected than other cities. Third, improving urban innovation ability and optimizing resource allocation are important ways through which high-tech zones influence urban economic resilience. These findings contribute significantly to the evaluation of the high-tech zones policy and form empirical evidence of the policy arrangements’ regional-level impact on economic resilience.
Application of the “Novel Foods” Regulation to Botanicals in the European Union
Authors: Morán Javier , Kilasoniya Alina
Published: February 2024

The European Union classifies “novel foods” as those not widely consumed before 15 May 1997. This category includes recently created, innovative foods, as well as those made using new technologies and processes, and foods with a traditional consumption history outside the EU. Distinguishing between “novel” and “conventional” foods is legally significant, as the former require official authorization under the Novel Foods Regulation. The regulation prioritizes safety, accurate labeling, and nutritional parity with replaced foods. Regulation (EU) 2015/2283, effective from 1 January 2018, replaced prior regulations, facilitating access to the EU market for novel and innovative foods while maintaining high safety standards. Classifying botanical products as novel can be intricate. Safety assessments for plant products must consider diversity in species, varieties, ecotypes, and chemotypes, as cultivation practices influence chemical composition. The article reviews the legislation applicable to botanicals and proposes different ways to evaluate in advance whether a product is “novel” or not, emphasizing the evaluation of the origin and consumption history of foods of plant origin.
Multifocality in Testicular Cancer: Clinicopathological Correlations and Prognostic Implications
Authors: Bumbasirevic Uros , Petrovic Milos , Zekovic Milica , Coric Vesna , Milojevic Bogomir , Lisicic Nikola , Obucina David , Vasilic Nenad , Bulat Petar , Zivkovic Marko , Cekerevac Milica , Bojanic Nebojsa , Janicic Aleksandar
Published: February 2024

There are limited data regarding the significance of multifocality in testicular cancer patients. This study evaluated the relationship between multifocality and clinicopathological features determined at the time of radical orchiectomy. The study involved 280 consecutive patients who underwent radical orchiectomy between 2018 and 2023. Multifocality was defined as a distinct tumor focus characterized by a group of malignant cells > 1 mm, clearly differentiated from the primary tumor mass. Uni- and multivariate logistic regression analyses were employed to investigate the association between multifocality and histopathological parameters along with potential risk factors for clinical stages II + III. Multifocality was identified in 44 (15.7%) patients. Significantly smaller primary tumors were observed in subjects with multifocality (20.0 mm vs. 30.0 mm, p = 0.0001), while those exhibiting monofocality presented a markedly elevated rate of tumors exceeding 4 cm (40.3% vs. 18.2%, p = 0.005). Furthermore, multifocality was associated with a significantly higher rate of primary tumors < 2 cm (52.3% vs. 29.2%, p = 0.003). Univariate logistic regression analysis revealed a substantial decrease in the likelihood of multifocality occurrence in seminoma patients with tumors > 4 cm (OR = 0.38, p = 0.017). Meanwhile, in multivariate logistic regression, multifocality did not emerge as a significant risk factor for clinical stages II + III in either seminoma (p = 0.381) or non-seminoma (p = 0.672) cases. Our study suggests that multifocality holds no substantial prognostic relevance for clinically advanced disease in testicular cancer patients. The findings indicate that multifocality is associated with smaller primary tumors, particularly those measuring less than 2 cm.
Lubrication Modeling of the Reciprocating Piston with High Lateral Load and Various Conditions in a Swash Plate-Type Piston Pump
Authors: Hong Sung-Ho , Shin Jung-Hun
Published: February 2024

Most asymmetrical lateral forces occur in the reciprocating piston mechanism, which is widely applied as a major component of power equipment. When this lateral force greatly acts on the piston, it comes into contact with the cylinder. To prevent this negative phenomenon, lubrication characteristic evaluation and control technology are necessary. In this study, a boundary lubrication model considering the elastic deformation of the contact surface was adopted to perform a lubrication analysis of a piston hydraulic pump widely used in the aviation and plant industries. The piston/cylinder mechanism was analyzed in terms of contact force, characteristic thickness, and power loss while varying various design and operating parameters (friction coefficient, clearance, profiling shape, operating speed, and pressure). In the overall bearing capacity to withstand the tilt of the piston, the bearing capacity ratio due to contact at the interface increased more steeply than the bearing capacity ratio in the fluid lubrication area. Profiling of the piston head played a positive role in reducing power loss but also increased piston tilt. This trend appeared more clearly as the head profiling degree of processing Increased. Lastly, the effects of variable operating speed and pressure were examined. High operating speed caused low contact force, and high operating pressure caused high contact force. Through this study, it was possible to predict the lubrication performance and power loss of reciprocating piston pumps used in the field more realistically through appropriate boundary lubrication modeling.
The Enhancement of Oil Delivery and Bearing Performance via a Guiding-Structured Nozzle under Oil–Air Lubrication
Authors: Zi Xintian , Chen Kai , Bai Qinghua , Li Xinming , Jin Xuyang , Wang Xu , Guo Feng
Published: February 2024

The oil–air lubrication method is specifically employed for high or ultra-high-speed spindle rolling bearings. Under high-speed conditions, the air curtain formed inside the bearing cavity obstructs oil delivery, thereby limiting further increases in spindle rotation speed. To enhance oil delivery capability, a guiding-structured nozzle has been developed to concentrate the jet flow and improve penetration through the air curtain. Tests were conducted on an oil–air lubricated bearing test bench to investigate the impact of nozzle structures and oil types on torque and temperature rise. The results demonstrate that compared to conventional nozzles, the guiding-structured nozzle requires smaller optimal amounts of oil supply, indicating its superior ability to deliver oil. Further examination of oil jet patterns and droplet distributions confirms that the guiding-structured nozzle provides a more concentrated jet flow with uniform distribution and smaller droplet sizes in diameter. These characteristics contribute to highly efficient oil delivery. Additionally, synthetic oils reduce droplet size, torque, and temperature rise in mixed lubrication regimes due to their formation of an anti-friction absorption layer on rubbing surfaces.
Microstructure and Mechanical Behavior Comparison between Cast and Additive Friction Stir-Deposited High-Entropy Alloy Al0.35CoCrFeNi
Authors: McClelland Zackery , Dunsford Kyle , Williams Brady , Petersen Haley , Devami Keivan , Weaver Mark , Jordan J. Brian , Allison Paul G.
Published: February 2024

High-entropy alloys (HEAs) are new alloy systems that leverage solid solution strengthening to develop high-strength structural materials. However, HEAs are typically cast alloys, which may suffer from large as-cast grains and entrapped porosity, allowing for opportunities to further refine the microstructure in a non-melting near-net shape solid-state additive manufacturing process, additive friction stir deposition (AFSD). The present research compares the microstructure and mechanical behavior of the as-deposited AFSD Al0.35CoCrFeNi to the cast heat-treated properties to assess its viability for structural applications for the first time. Scanning electron microscopy (SEM) revealed the development of fine particles along the layer interfaces of the deposit. Quasi-static and intermediate-rate compression testing of the deposited material revealed a significant strain-rate sensitivity with a difference in yield strength of ~400 MPa. Overall, the AFSD process greatly reduced the grain size for the Al0.35CoCrFeNi alloy and approximately doubled the strength at both quasi-static and intermediate strain rates.
Modeling Dendrite Coarsening and Remelting during Directional Solidification of Al-06wt.%Cu Alloy
Authors: Sari Ibrahim , Alrasheedi Nashmi , Ahmadein Mahmoud , Djuansjah Joy , Hachani Lakhdar , Zaidat Kader , Wu Menghuai , Kharicha Abdellah
Published: February 2024

Research efforts have been dedicated to predicting microstructural evolution during solidification processes. The main secondary arm spacing controls the mushy zone’s permeability. The aim of the current work was to build a simple sub-grid model that describes the growth and coarsening of secondary side dendrite arms. The idea was to reduce the complexity of the curvature distribution with only two adjacent side arms in concurrence. The model was built and applied to the directional solidification of Al-06wt%Cu alloy in a Bridgman experiment. The model showed its effectiveness in predicting coarsening phenomena during the solidification of Al-06wt%Cu alloy. The results showed a rapid growth of both arms at an earlier stage of solidification, followed by the remelting of the smaller arm. In addition, the results are in good agreement with an available time-dependent expression which covers the growth and coarsening. Such model can be implemented as a sub-grid model in volume average models for the prediction of the evolution of the main secondary arms spacing during macroscopic solidification processes.
Fabrication and Characterization of Al2O3-Siloxane Composite Thermal Pads for Thermal Interface Materials
Authors: Kim Seul-Ki , Koo Yeong-Jin , Kim Hyun Sik , Lee Jong-Keun , Jeong Kyounghoon , Lee Younki , Jung Eun Young
Published: February 2024

In this study, Al2O3–siloxane composite thermal pads were fabricated using a tape–casting technique, and the thermal conductivity effect of the Al2O3 nanoparticle powder synthesized using a flame fusion process on siloxane composite thermal pads was investigated. Furthermore, various case studies were implemented, wherein the synthesized Al2O3 nanoparticle powder was subjected to different surface treatments, including dehydration, decarbonization, and silylation, to obtain Al2O3–siloxane composite thermal pads with high thermal conductivity. The experimental results confirmed that the thermal conductivity of the Al2O3–siloxane composite pads improved when fabricated using surface–treated Al2O3 nanoparticle powder synthesized with an optimally spheroidized crystal structure compared to that produced using non–treated Al2O3 nanoparticle powder. Therefore, this study provides guidelines for fabricating Al2O3–siloxane composite thermal pads with high thermal conductivity in the field of thermal interface materials.
Surface Polishing of an Inconel 625 Bar by a Super-Fast MAF Process for a Solenoid Valve Stem Used in a Hydrogen Tank
Authors: Kim Hwi-Joong , Heng Lida , Mun Sang-Don
Published: February 2024

This study explores a super-fast magnetic abrasive finishing (MAF) process for polishing the surface of an Inconel 625 bar workpiece for a hydrogen solenoid valve stem. The Inconel 625 bar was chosen to replace the existing STS 316 bar material, previously used for a hydrogen solenoid valve stem. The cylindrical surface of Inconel 625 bars was polished by a super-fast MAF process with high rotational speeds of 1000, 5000, 15,000, and 25,000 RPM and a super-strong magnetic field of 550 mT. The polishing characteristics of this process were evaluated according to the type of abrasives, rotational speeds of the workpiece and processing time. As a result, a super-smooth Inconel 625 bar was successfully achieved, with a surface roughness (Ra) reduced from 0.31 μm to 0.02 μm under the optimal conditions (15,000 RPM, CNT particles (0.04 μm), PCD diamond abrasive (1 μm), Fe (#200), 0.5 g of light oil, and 16 min of processing time). Also, the Ansys analysis results showed suitable strain, equivalent stress, and safety factor of the Inconel 625 bar. This confirmed that, after a super-fast MAF process, an Inconel 625 bar is feasible for application in Hydrogen (H2) tanks instead of a conventional STS 316 bar.
Circadian Disruption across Lifespan Impairs Glucose Homeostasis and Insulin Sensitivity in Adult Mice
Authors: Her Tracy K. , Li Jin , Lin Hao , Liu Dong , Root Kate M. , Regal Jean F. , Alejandro Emilyn U. , Cao Ruifeng
Published: February 2024

Circadian rhythm disruption is associated with impaired glucose homeostasis and type 2 diabetes. For example, night shift work is associated with an increased risk of gestational diabetes. However, the effects of chronic circadian disruption since early life on adult metabolic health trajectory remain unknown. Here, using the “Short Day” (SD) mouse model, in which an 8 h/8 h light/dark (LD) cycle was used to disrupt mouse circadian rhythms across the lifespan, we investigated glucose homeostasis in adult mice. Adult SD mice were fully entrained into the 8 h/8 h LD cycle, and control mice were entrained into the 12 h/12 h LD cycle. Under a normal chow diet, female and male SD mice displayed a normal body weight trajectory. However, female but not male SD mice under a normal chow diet displayed glucose intolerance and insulin resistance, which are associated with impaired insulin signaling/AKT in the skeletal muscle and liver. Under high-fat diet (HFD) challenges, male but not female SD mice demonstrated increased body weight gain compared to controls. Both male and female SD mice developed glucose intolerance under HFD. Taken together, these results demonstrate that environmental disruption of circadian rhythms contributes to obesity in a sexually dimorphic manner but increases the risk of glucose intolerance and insulin resistance in both males and females.
Phytocompounds and Regulation of Flavonoids in In Vitro-Grown Safflower Plant Tissue by Abiotic Elicitor CdCl2
Authors: Ejaz Bushra , Mujib Abdul , Syeed Rukaya , Mamgain Jyoti , Malik Moien Qadir , Birat Kanchan , Dewir Yaser Hassan , Magyar-Tábori Katalin
Published: February 2024

In this study, a Gas chromatography–mass spectrometry (GC–MS) investigation of embryogenic callus and somatic embryo regenerated shoots of Carthamus tinctorius revealed the presence of a variety of sugars, sugar acids, sugar alcohols, fatty acids, organic acids, and amino acids of broad therapeutic value. The in vitro developed inflorescence contained a wide range of active compounds. In embryogenic calluses, important flavonoids like naringenin, myricetin, kaempferol, epicatechin gallate, rutin, pelargonidin, peonidin, and delphinidin were identified. To augment the synthesis of active compounds, the effect of cadmium chloride (CdCl2) elicitation was tested for various treatments (T1–T4) along with a control (T0). Varying concentrations of CdCl2 [0.05 mM (T1), 0.10 mM (T2), 0.15 mM (T3), and 0.20 mM (T4)] were added to the MS medium, and flavonoid accumulation was quantified through ultra-high-pressure liquid chromatography–tandem mass spectroscopy (UHPLC–MS/MS). The flavonoids naringenin, kaempferol, epicatechin gallate, pelargonidin, cyanidin, and delphinidin increased by 6.7-, 1.9-, 3.3-, 2.1-, 1.9-, and 4.4-fold, respectively, at T3, whereas quercetin, myricetin, rutin, and peonidin showed a linear increase with the increase in CdCl2 levels. The impacts of stress markers, i.e., ascorbate peroxidase (APX), catalase (CAT), and superoxide dismutase (SOD), on defense responses in triggering synthesis were also evaluated. The maximum APX and SOD activity was observed at T3, while CAT activity was at its maximum at T2. The impact of elicitor on biochemical attributes like protein, proline, sugar, and malondialdehyde (MDA) content was investigated. The maximum protein, proline, and sugar accumulation was noted at high elicitor dose T4, while the maximum MDA content was noted at T3. These elevated levels of biochemical parameters indicated stress in culture, and the amendment of CdCl2 in media thus could be a realistic approach for enhancing secondary metabolite synthesis in safflower.
Cross-Phosphorylation between AgrC Histidine Kinase and the Noncognate Response Regulator Lmo1172 in Listeria monocytogenes under Benzalkonium Chloride Stress
Authors: Yu Tao , Jiang Xiaojie , Xu Xiaobo , Xu Ping , Qiu Shuxing , Yin Junlei , Hamilton David P. , Jiang Xiaobing
Published: February 2024

Benzalkonium chloride (BC) is widely used for disinfection in the food industry. However, Listeria monocytogenes strains with resistance to BC have been reported recently. In L. monocytogenes, the Agr communication system consists of a membrane-bound peptidase AgrB, a precursor peptide AgrD, a histidine kinase (HK) AgrC, and a response regulator (RR) AgrA. Our previous study showed that the agr genes are significantly upregulated by BC adaptation. This study aimed to investigate the role of the Agr system in BC resistance in L. monocytogenes. Our results showed that the Agr system was involved in BC resistance. However, a direct interaction between BC and AgrC was not observed, nor between BC and AgrA. These results indicated that BC could induce the Agr system via an indirect action. Both AgrBD and AgrC were required for growth under BC stress. Nevertheless, when exposed to BC, the gene deletion mutant ∆agrA strain exhibited better growth performance than its parental strain. The RR Lmo1172 played a role in BC resistance in the ∆agrA strain, suggesting that Lmo1172 may be an alternative to AgrA in the phosphotransfer pathway. Phosphorylation of Lmo1172 by AgrC was observed in vitro. The cognate HK Lmo1173 of Lmo1172 was not involved in BC stress, regardless of whether it was as the wild-type or the ∆agrA mutant strain. Our evidence suggests that the HK AgrC cross-phosphorylates its noncognate RR Lmo1172 to cope with BC stress when the cognate RR AgrA is absent. In vivo, further studies will be required to detect phosphotransfer of AgrC/AgrA and AgrC/Lmo1172.
Analysis of Antibiotic Resistance Genes in Water Reservoirs and Related Wastewater from Animal Farms in Central China
Authors: Rui Yapei , Qiu Gang
Published: February 2024

This study aimed to explore the phenotype and relationship of drug resistance genes in livestock and poultry farm wastewater and drinking water reservoirs to provide evidence for the transmission mechanisms of drug resistance genes, in order to reveal the spread of drug resistance genes in wastewater from intensive farms in Central China to urban reservoirs that serve as drinking water sources and provide preliminary data for the treatment of wastewater from animal farms to reduce the threat to human beings. DNA extraction and metagenomic sequencing were performed on eight groups of samples collected from four water reservoirs and four related wastewaters from animal farms in Central China. Metagenomic sequencing showed that the top 20 AROs with the highest abundance were vanT_gene, vanY_gene, adeF, qacG, Mtub_rpsL_STR, vanY_gene_, vanW_gene, Mtub_murA_FOF, vanY_gene, vanH_gene, FosG, rsmA, qacJ, RbpA, vanW_gene, aadA6, vanY_gene, sul4, sul1, and InuF. The resistance genes mentioned above belong to the following categories of drug resistance mechanisms: antibiotic target replacement, antibiotic target protection, antibiotic inactivation, and antibiotic efflux. The resistomes that match the top 20 genes are Streptococcus agalactiae and Streptococcus anginosus; Enterococcus faecalis; Enterococcus faecium; Actinomyces viscosus and Bacillus cereus. Enterococcus faecium; Clostridium tetani; Streptococcus agalactiae and Streptococcus anginosus; Streptococcus agalactiae and Streptococcus anginosus; Acinetobacter baumannii, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Corynebacterium jeikeium, Corynebacterium urealyticum, Mycobacterium kansasii, Mycobacterium tuberculosis, Schaalia odontolytica, and Trueperella pyogenes; Mycobacterium avium and Mycobacterium tuberculosis; Aeromonas caviae, Enterobacter hormaechei, Vibrio cholerae, Vibrio metoecus, Vibrio parahaemolyticus, and Vibrio vulnificus; Pseudomonas aeruginosa and Pseudomonas fluorescens; Staphylococcus aureus and Staphylococcus equorum; M. avium, Achromobacter xylosoxidans, and Acinetobacter baumannii; Sphingobium yanoikuyae, Acinetobacter indicus, Morganella morganii, Proteus mirabilis, Proteus vulgaris, Providencia rettgeri, and Providencia stuartii. Unreported drug resistance genes and drug-resistant bacteria in Central China were identified in 2023. In the transmission path of drug resistance genes, the transmission path from aquaculture wastewater to human drinking water sources cannot be ignored. For the sake of human health and ecological balance, the treatment of aquaculture wastewater needs to be further strengthened, and the effective blocking of drug resistance gene transmission needs to be considered.
Porphyrin-Based Molecules in the Fossil Record Shed Light on the Evolution of Life
Authors: Ayala Juan D. , Schroeter Elena R. , Schweitzer Mary H.
Published: February 2024

The fossil record demonstrates the preservation of porphyrins (e.g., heme) in organic sediments and the fossilized remains of animals. These molecules are essential components in modern metabolic processes, such as electron transport (cytochromes) and oxygen transport (hemoglobin), and likely originated before the emergence of life. The integration and adaptation of porphyrins and structurally similar molecules (e.g., chlorophylls) are key aspects in the evolution of energy production (i.e., aerobic respiration and photosynthesis) and complex life (i.e., eukaryotes and multicellularity). Here, we discuss the evolution and functional diversity of heme-bound hemoglobin proteins in vertebrates, along with the preservation of these molecules in the fossil record. By elucidating the pivotal role of these molecules in the evolution of life, this review lays the groundwork necessary to explore hemoglobin as a means to investigate the paleobiology of extinct taxa, including non-avian dinosaurs.
CO2-Driven N-Formylation/N-Methylation of Amines Using C-Scorpionate Metal Complexes
Authors: Matias Inês A. S. , Trzeciak Anna M. , Pąchalska Paulina , Ribeiro Ana P. C. , Martins Luísa M. D. R. S.
Published: February 2024

C-scorpionate metal complexes, specifically, [NiCl2(tpm)]·3H2O, [CoCl2(tpm)]·3H2O and [PdCl2(tpm)] [tpm = hydrotris(1H-pyrazol-1-yl)methane], were effective in the N-formylation and N-methylation of amines using carbon dioxide, as carbon source, in the presence of sodium borohydride. Various parameters were studied, including reaction time, temperature, solvent volume, presence of additives, and catalyst amount. These parameters were found to have a significant impact on the selectivity of the product. [NiCl2(tpm)]·3H2O exhibited good conversion at 80 °C, but its selectivity towards formamide decreased with prolonged reaction time. Increasing the amount of [NiCl2(tpm)]·3H2O, the selectivity changed. [PdCl2(tpm)] showed different selectivity compared to [NiCl2(tpm)]·3H2O, while [CoCl2(tpm)]·3H2O presented poor results. Monitoring the reaction course by 1H NMR revealed the presence of an intermediate species that influenced product formation. These results highlight the versatility and catalytic potential of C-scorpionate metal complexes in the N-formylation/N-methylation of amines in the catalytic system (NaBH4/MeCN/CO2).
Synergistic Charge Transfer Effect in Ferrous Heme–CO Bonding within Cytochrome P450
Authors: Zhang Enhua , Hirao Hajime
Published: February 2024

We conducted ab initio valence bond (VB) calculations employing the valence bond self-consistent field (VBSCF) and breathing orbital valence bond (BOVB) methods to investigate the nature of the coordination bonding between ferrous heme and carbon monoxide (CO) within cytochrome P450. These calculations revealed the significant influence exerted by both proximal and equatorial ligands on the π-backdonation effect from the heme to the CO. Moreover, our VB calculations unveiled a phenomenon of synergistic charge transfer (sCT). In the case of ferrous heme–CO bonding, the significant stabilization in this sCT arises from cooperative resonance between the VB structures associated with σ donation and π backdonation. Unlike many other ligands, CO possesses the unique ability to establish two mutually perpendicular π-backdonation orbital interaction pairs, leading to an intensified stabilization attributed to σ–π resonance. Furthermore, while of a smaller energy magnitude, sCT due to one π–π pair is also present, contributing to the differential stabilization of ferrous heme–CO bonding.
Strophanthidin Induces Apoptosis of Human Lung Adenocarcinoma Cells by Promoting TRAIL-DR5 Signaling
Authors: Tian Xiao , Gu Liangzhen , Zeng Fangang , Liu Xingkai , Zhou Yang , Dou Yang , Han Juanjuan , Zhao Yao , Zhang Yanyan , Luo Qun , Wang Fuyi
Published: February 2024

Strophanthidin (SPTD), one of the cardiac glycosides, is refined from traditional Chinese medicines such as Semen Lepidii and Antiaris toxicaria, and was initially used for the treatment of heart failure disease in clinic. Recently, SPTD has been shown to be a potential anticancer agent, but the underlying mechanism of action is poorly understood. Herein, we explored the molecular mechanism by which SPTD exerts anticancer effects in A549 human lung adenocarcinoma cells by means of mass spectrometry-based quantitative proteomics in combination with bioinformatics analysis. We revealed that SPTD promoted the expression of tumor necrosis factor (TNF)-related apoptosis-inducing ligand receptor 2 (TRAIL-R2, or DR5) in A549 cells to activate caspase 3/6/8, in particular caspase 3. Consequently, the activated caspases elevated the expression level of apoptotic chromatin condensation inducer in the nucleus (ACIN1) and prelamin-A/C (LMNA), ultimately inducing apoptosis via cooperation with the SPTD-induced overexpressed barrier-to-autointegration factor 1 (Banf1). Moreover, the SPTD-induced DEPs interacted with each other to downregulate the p38 MAPK/ERK signaling, contributing to the SPTD inhibition of the growth of A549 cells. Additionally, the downregulation of collagen COL1A5 by SPTD was another anticancer benefit of SPTD through the modulation of the cell microenvironment.
Sub-Diffraction Readout Method of High-Capacity Optical Data Storage Based on Polarization Modulation
Authors: Zhang Li , Li Wenwen , Wang Zhongyang
Published: February 2024

The big data era demands an efficient and permanent data storage technology with the capacity of PB to EB scale. Optical data storage (ODS) offers a good candidate for long-lifetime storage, as the developing far-field super-resolution nanoscale writing technology improves its capacity to the PB scale. However, methods to efficiently read out this intensive ODS data are still lacking. In this paper, we demonstrate a sub-diffraction readout method based on polarization modulation, which experimentally achieves the sub-diffraction readout on Disperse Red 13 thin film with a resolution of 500 nm, exceeding the diffraction limit by 1.2 times (NA = 0.5). Differing from conventional binary encoding, we propose a specific polarization encoding method that enhances the capacity of ODS by 1.5 times. In the simulation, our method provides an optical data storage readout resolution of 150 nm, potentially to 70 nm, equivalent to 1.1 PB in a DVD-sized disk. This sub-diffraction readout method has great potential as a powerful readout tool for next-generation optical data storage.
Unveiling Fundamentals of Multi-Beam Pulsed Laser Ablation in Liquids toward Scaling up Nanoparticle Production
Authors: Gatsa Oleksandr , Tahir Shabbir , Flimelová Miroslava , Riahi Farbod , Doñate-Buendia Carlos , Gökce Bilal , Bulgakov Alexander V.
Published: February 2024

Pulsed laser ablation in liquids (PLAL) is a versatile technique to produce high-purity colloidal nanoparticles. Despite considerable recent progress in increasing the productivity of the technique, there is still significant demand for a practical, cost-effective method for upscaling PLAL synthesis. Here we employ and unveil the fundamentals of multi-beam (MB) PLAL. The MB-PLAL upscaling approach can bypass the cavitation bubble, the main limiting factor of PLAL efficiency, by splitting the laser beam into several beams using static diffractive optical elements (DOEs). A multimetallic high-entropy alloy CrFeCoNiMn was used as a model material and the productivity of its nanoparticles in the MB-PLAL setup was investigated and compared with that in the standard single-beam PLAL. We demonstrate that the proposed multi-beam method helps to bypass the cavitation bubble both temporally (lower pulse repetition rates can be used while keeping the optimum processing fluence) and spatially (lower beam scanning speeds are needed) and thus dramatically increases the nanoparticle yield. Time-resolved imaging of the cavitation bubble was performed to correlate the observed production efficiencies with the bubble bypassing. The results suggest that nanoparticle PLAL productivity at the level of g/h can be achieved by the proposed multi-beam strategy using compact kW-class lasers and simple inexpensive scanning systems.
Enhancing Maize Yield and Soil Health through the Residual Impact of Nanomaterials in Contaminated Soils to Sustain Food
Authors: Mahmoud Esawy , El-shahawy Asmaa , Ibrahim Mahmoud , Abd El-Halim Abd El-Halim A. , Abo-Ogiala Atef , Shokr Mohamed. S. , Mohamed Elsayed Said , Rebouh Nazih Y. , Ismail Sahar Mohamed
Published: February 2024

Studying the impact of residual soil nanomaterials is a promising challenge for sustainable agricultural development to improve soil health and crop productivity. The objective of this study is to assess the long-term impacts of 50, 100, and 250 mg kg−1 soil of nanobiochar (nB) and nano-water treatment residues (nWTR) on the fertility, biological activity, and yield of maize (Zea mays L.) growing in heavy metal-contaminated soils. The results showed that when nB and nWTR were added in larger quantities, the concentrations of lead (Pb), nickel (Ni), cadmium (Cd), and cobalt (Co) extracted with DTPA decreased. With the addition of nB or nWTR, it also showed a significant increase in exchangeable cations, cation exchange capacity (CEC), soil fertility, soil organic matter (OM), microbial biomass carbon (MBC), and a decrease in soil salinity and sodicity. Catalase and dehydrogenase activities rose as nB addition increased, while they decreased when nWTR addition increased. In comparison to the control, the addition of nB and nWTR greatly boosted maize yield by 54.5–61.4% and 61.9–71.4%, respectively. These findings suggest that the researched nanomaterials’ residual effect provides an eco-friendly farming method to enhance the qualities of damaged soils and boost maize production. Our research suggested that adding recycling waste in the form of nanoparticles could immobilize heavy metals, improve soil characteristics, and increase the soil’s capacity for productivity.
Obesity as a Risk Factor for Complications and Mortality in Individuals with SARS-CoV-2: A Systematic Review
Authors: de Paula Silva-Lalucci Marielle Priscila , Marques Déborah Cristina de Souza , Valdés-Badilla Pablo , Andreato Leonardo Vidal , Magnani Branco Braulio Henrique
Published: February 2024

This systematic review aimed to analyze the available studies that identified overweight and/or obesity as a risk factor for mortality, use of respiratory support, and changes in biochemical markers in adults hospitalized with SARS-CoV-2. The PubMed, Web of Science, and Scopus databases were searched using PRISMA guidelines until January 2024. The protocol was registered with PROSPERO (code: CRD42024501551). Of the 473 articles, only 8 met the inclusion criteria (e.g., adult individuals aged 18 or over diagnosed with COVID-19 individuals with overweight and/or obesity). In addition, the Downs and Black tool was used to assess the quality of the studies. The studies analyzed totaled 9782 adults hospitalized for COVID-19, indicating that overweight and obesity are present in more than half of adults. Diseases such as diabetes mellitus and hypertension are more prevalent in adults with obesity. The systematic review also highlighted that a higher incidence of respiratory support is related to a higher incidence of hospitalization in intensive care units and that adults with overweight and obesity have a higher risk of mortality from COVID-19. Biochemical markers such as procalcitinin, C-reactive protein, and interleukin-6 are associated with the severity of COVID-19 infection. This systematic review exposed overweight and/or obesity as a risk factor for worse COVID-19 disease, as well as for the need for intensive care, respiratory support, mortality, and changes in essential blood markers.