bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiple Phase Change Material (PCM) Configuration for PCM-Based Heat Sinks—An Experimental Study
Authors: Al Siyabi Idris , Khanna Sourav , Mallick Tapas , Sundaram Senthilarasu
Published: June 2018

A small-scale phase change material (PCM)-based heat sink can regulate the temperature of electronics due to high latent-heat capacity. Three different heat sinks are examined to study the effects of PCM combination, arrangement of PCMs in multiple-PCM heat sink, PCM thickness, melting temperature and intensity of heat source on the thermal behavior of heat sink. Results are obtained for the temperature distribution across the heat sink and the PCM melting profile. It is concluded that (i) PCM combination RT50–RT55 increases the thermal regulation period and also reduces the heat sink temperature at the end of the operation, (ii) the RT58–RT47 arrangement slightly reduces the maximum temperature as compared to RT47–RT58, (iii) As PCM thickness increases from 30 mm to 60 mm, the thermal-regulation-period increases by 50 min, (iv) As the PCM melting temperature increases, the thermal-regulation-period and the heat sink temperature increase and (v) The thermal-regulation-period decreases as the power rating increases from 1 to 2 W.
Spatial Distribution of Wildfires Ignited under Katabatic versus Non-Katabatic Winds in Mediterranean Southern California USA
Authors: Kolden Crystal A. , Abatzoglou John T.
Published: June 2018

Wildfires are a major hazard to humans in the southern California Mediterranean ecosystem and improving our understanding and delineation of different fire regimes is critical to mitigating wildfire-related hazards. Recent research has demonstrated that there are two distinct fire regimes in this region based on the presence or absence of katabatic winds (primarily Santa Ana winds) concurrent with the fire. Here, we expand the katabatic wind category to include Sundowner winds along the Santa Barbara front range and analyze the spatial relationships and difference in ignition sources between fires associated with katabatic and non-katabatic wind events from 1948–2017. We found distinct spatial extents for katabatic versus non-katabatic fires, with areas of the higher number of repeat fires generally associated with one fire type or the other. These spatial delineations were consistent with prior analyses of katabatic wind patterns and were also related to the climatology of marine influences across the region. Finally, we contextualize the burn perimeter of the 2017 Thomas Fire, the largest fire in modern California history, relative to spatial patterns of katabatic and non-katabatic fires. The 2017 Thomas Fire began during the longest Santa Ana event in the last 70 years in an area that has been burned repeatedly by Santa Ana fires. However, the Thomas Fire ultimately burned into a region where there were no prior Santa Ana fires. The spatial delineation of two relatively distinct fire regimes is critical to making management decisions, such as where to locate suppression resources at critical times and where fuel treatments might be most effective. However, the anomalous pattern of the Thomas Fire also points to the potential for changes in anthropogenic and environmental factors to disrupt historical spatial patterns and suggests that spatial patterns of fire regimes are themselves prospective metrics of global change.
Sulfur, Strontium, Carbon, and Oxygen Isotopes of Calcium Sulfate Deposits in Late Carboniferous Rocks of the Loei-Wang Saphung (LWS) Area, Loei Province, Thailand
Authors: Surakotra Nusara , Promkotra Sarunya , Charusiri Punya , Maruoka Teruyuki , Hisada Ken-inchiro
Published: June 2018

Sulfate deposits of the Loei-Wang Saphung (LWS) area, northeastern Thailand, intercalated with carbonate and silicic clastic rock were analyzed for S, O, C, and Sr to determine the depositional environment, as well as the age of formation. Sulfate samples yielded average values of δ34S of 14.6‰, while the 87Sr/86Sr ratio of gypsum was 0.708282 and that of anhydrite was 0.708288. The carbonate layers yielded average δ18OPDB, and δ13C values of −12.5‰ and −0.1‰, respectively. Our results revealed that the LWS evaporite deposits were originally formed from seawater, and the relatively negative value of δ18O was a result of meteoric alteration during subaerial exposure of the sections. Comparing these isotopic values with the nearby Nakon Sawan sulfate deposits, the Sr isotopes showed slightly higher values with very mild variations. These isotopic values suggest that the LWS deposits were not affected by subsequent hydrothermal alteration by younger igneous dikes in this area. Therefore, some of these isotope signatures are considered to be primary features of the deposit, despite the fact that the deposit underwent anchizone to epizone metamorphism. The S and Sr isotope values support the depositional age of the LWS sulfate deposit in the Middle to Late Carboniferous.
A Qualitative Exploratory Study of Informal Carers’ Experiences of Identifying and Managing Oral Pain and Discomfort in Community-Dwelling Older People Living with Dementia
Authors: Newton Paul , Curl Charlotte , Prasad Ria , Pass Patricia , Bowden Julie
Published: June 2018

Increased prevalence of dementia and poor oral health in older people is associated with more people living with dementia who experience oral pain and discomfort. However, little is known about how informal carers manage oral pain for people living with dementia in the community. This study aimed to explore informal carers’ experiences of identifying and managing oral pain and discomfort in people living with dementia, and barriers and enablers they encountered. Focus groups with informal carers of people living with dementia were conducted, transcribed verbatim, and analysed using thematic analysis. Carers’ accounts suggested that day-to-day contact was required to identify oral pain and discomfort, and a symptomology of the signs and symptoms was developed. Carers’ accounts also highlighted issues in maintaining oral health, difficulties in accessing the mouth, managing dentures, competing demands, and difficulties in accessing treatment due to health service-, behavioural- and treatment- related barriers. Enablers included informal carers’ pivotal role in the identifying and managing oral pain and discomfort in people living with dementia. The study concludes that carers want more partnership work with dental professionals, and clearer care pathways are required to meet the oral health needs of people living with dementia who experience oral pain.
Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater
Authors: Durso Lisa M. , Miller Daniel N. , Henry Christopher G.
Published: June 2018

Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to enumerate tetracycline and cefotaxime-resistant bacteria. Enterobacteriaceae isolates (n = 822) were characterized for carriage of tetracycline resistance genes, and E. coli isolates (n = 673) were phenotyped to determine multi-drug resistance (MDR) profiles. Tetracycline resistance in feedlot runoff wastewater was 2-to-3 orders of magnitude higher compared to rainfall runoff from the VTS fields, indicating efficacy of the VTA for reducing ARB over time following wastewater application. Clear differences in MDR profiles were observed based on the specific media on which a sample was plated. This result highlights the importance of method, especially in the context of isolate-based surveillance and monitoring of ARB in agricultural wastewaters.
Solid Waste Management Solutions for a Rapidly Urbanizing Area in Thailand: Recommendations Based on Stakeholder Input
Authors: Yukalang Nachalida , Clarke Beverley , Ross Kirstin
Published: June 2018

Municipal solid waste is a significant problem, particularly in developing countries that lack sufficient infrastructure and useable land mass to process it in an appropriate manner. Some developing nations are experiencing a combination of issues that prevent proper management of solid waste. This paper reviews the management of municipal solid waste in northeast Thailand, using the Tha Khon Yang Sub-district Municipality (TKYSM) in Maha Sarakham Province as a case study. The combination of rapid population and economic growth and its associated affluence has led to an increase in the use of consumer items and a concomitant increase in the production of municipal solid waste. In the TKYSM there is pressure on local government to establish a suitable waste management program to resolve the escalating waste crisis. The aim of this study is to provide viable solutions to waste management challenges in the TKYSM, and potentially to offer guidance to other similar localities also facing the same challenges. It is well established that successful changes to waste management require an understanding of local context and consideration of specific issues within a region. Therefore, extensive community consultation and engagement with local experts was undertaken to develop an understanding of the particular waste management challenges of the TKYSM. Research methods included observations, one-on-one interviews and focus groups with a range of different stakeholders. The outcomes of this research highlight a number of opportunities to improve local infrastructure and operational capacity around solid waste management. Waste management in rural and urban areas needs to be approached differently. Solutions include: development of appropriate policy and implementation plans (based around the recommendations of this paper); reduction of the volume of waste going to landfill by establishing a waste separation system; initiation of a collection service that supports waste separation at source; educating the citizens of the municipality; and the local government staff, and for the local government to seek external support from the local temples and expertise from the nearby university.
The Three Gorges Dam: Does the Flooding Time Determine the Distribution of Schistosome-Transmitting Snails in the Middle and Lower Reaches of the Yangtze River, China?
Authors: Yang Yu , Zheng Sheng-Bang , Yang Ya , Cheng Wan-Ting , Pan Xiang , Dai Qing-Qing , Chen Yue , Zhu Lan , Jiang Qing-Wu , Zhou Yi-Biao
Published: June 2018

Background: Schistosomiasis is one of the most devastating tropical diseases in the world. Oncomelania hupensis is the only intermediate host of Schistosoma japonicum, and its growth and development are sensitive to environmental factors. The Three Gorges Dam has substantially altered the water level in the Yangtze River. This study focused on the impact of the flooding time on the occurrence of Oncomelania snails in Hunan Province, China. Methods: The data regarding Oncomelania snails were collected from the Schistosomiasis Atlas of the People’s Republic of China. Air temperature, hours of daylight and relative humidity from 1995 to 2002 were collected from the China Meteorological Data Sharing Service System. The data for rainfall and days inundated with water were collected from the Hunan flood control information system and hydrological stations in Hunan Province. A generalized additive model was used to estimate the impact of these factors on the presence or absence of snails. Results: The number of days inundated with water in the areas with snails ranged from 56 to 212 days. However, 82 percent of the areas without snails were inundated with water less than 60 days. The lowest air temperature in a year in the areas without snails ranges from −2.88 °C to −2.10 °C, and the range was from −2.88 °C to −2.34 °C for areas with snails. Annual rainfall in the areas with snails ranged from 989 to 1565 mm, and the range was from 1230 mm to 1647 mm for the areas without snails. The results from the generalized additive model showed that the number of days inundated with water, lowest air temperature in a year, annual rainfall, days of daily rainfall greater than 0.1 mm, and hours of daylight were the factors that significantly affect the occurrence of snails in Hunan Province, China. Conclusions: The number of days inundated with water may be a key factor determining the geographical distribution of Oncomelania snails in Hunan Province and the favorable number of days inundated with water for the survival of snails ranges from about 2 to 7 months.
A Scalable Architecture for Real-Time Stream Processing of Spatiotemporal IoT Stream Data—Performance Analysis on the Example of Map Matching
Authors: Laska Marius , Herle Stefan , Klamma Ralf , Blankenbach Jörg
Published: June 2018

Scalable real-time processing of large amounts of data has become a research topic of particular importance due to the continuously rising amount of data that is generated by devices equipped with sensing components. While existing approaches allow for fault-tolerant and scalable stream processing, we present a pipeline architecture that consists of well-known open source tools to specifically integrate spatiotemporal internet of things (IoT) data streams. In a case study, we utilize the architecture to tackle the online map matching problem, a pre-processing step for trajectory mining algorithms. Given the rising amount of vehicle location data that is generated on a daily basis, existing map matching algorithms have to be implemented in a distributed manner to be executable in a stream processing framework that provides scalability. We demonstrate how to implement state-of-the-art map matching algorithms in our distributed stream processing pipeline and analyze measured latencies.
Comic Strip Narratives in Time Geography
Authors: Moore Antoni B. , Nowostawski Mariusz , Frantz Christopher , Hulbe Christina
Published: June 2018

On the basis of a shared emphasis on time as well as space, this paper argues for introducing elements of comic art into cartography, specifically the mapped comic, with an illustrated strip literally plotted and placed in a 3D time geographic virtual world. This approach is situated within current initiatives regarding the relationship between cartography and art, given that comics are a type of sequential art. Two examples demonstrate that the approach succeeds as a way of representing the geometry of a story without compromising emotional content. Comic conventions neatly package narrative geography for visual deployment. An example demonstrating the expressiveness of comic art principles when applied to maps (maps as comics) is discussed.
Bosutinib Inhibits EGFR Activation in Head and Neck Cancer
Authors: Segrelles Carmen , Contreras David , Navarro Elena M. , Gutiérrez-Muñoz Carmen , García-Escudero Ramón , Paramio Jesús M. , Lorz Corina
Published: June 2018

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide, and although new therapeutic approaches have been recently evaluated, overall patient survival is still poor. Thus, new effective and selective clinical treatments are urgently needed. An analysis of data from large-scale, high-throughput drug screening cell line projects identified Bosutinib, a Src/Abl inhibitor that is currently used for the treatment of chronic myelogenous leukemia, as a candidate drug to treat HNSCC. Using a panel of HNSCC-derived cell lines, we found that treatment with Bosutinib reduced cell proliferation and induced apoptosis of sensitive cell lines. The drug rapidly inhibited Src and EGFR (epidermal growth factor receptor) phosphorylation, and sensitivity to Bosutinib was correlated with the activation status of EGFR. Similar findings were observed in in vivo xenograft assays using HNSCC derived cells. Moreover, in the presence of mutations in PIK3CA, the combination of Bosutinib with the PI3Kα inhibitor Alpelisib showed a synergistic effect. These results suggest that Bosutinib could be a new effective drug for the treatment of HNSCC, particularly in tumors with high EGFR activity. Its combination with Alpelisib could especially benefit patients bearing activating mutations of PIK3CA.
Targeting mTOR as a Therapeutic Approach in Medulloblastoma
Authors: Aldaregia Juncal , Odriozola Ainitze , Matheu Ander , Garcia Idoia
Published: June 2018

Mechanistic target of rapamycin (mTOR) is a master signaling pathway that regulates organismal growth and homeostasis, because of its implication in protein and lipid synthesis, and in the control of the cell cycle and the cellular metabolism. Moreover, it is necessary in cerebellar development and stem cell pluripotency maintenance. Its deregulation has been implicated in the medulloblastoma and in medulloblastoma stem cells (MBSCs). Medulloblastoma is the most common malignant solid tumor in childhood. The current therapies have improved the overall survival but they carry serious side effects, such as permanent neurological sequelae and disability. Recent studies have given rise to a new molecular classification of the subgroups of medulloblastoma, specifying 12 different subtypes containing novel potential therapeutic targets. In this review we propose the targeting of mTOR, in combination with current therapies, as a promising novel therapeutic approach.
Post-Receptor Inhibitors of the GHR-JAK2-STAT Pathway in the Growth Hormone Signal Transduction
Authors: Wójcik Maciej , Krawczyńska Agata , Antushevich Hanna , Herman Andrzej Przemysław
Published: June 2018

The growth hormone (GH) plays a key role in the regulation of metabolic processes in an organism. Determination of the correct structure and functioning of the growth hormone receptor (GHR) allowed for a more detailed research of its post-receptor regulators, which substantially influences its signal transduction. This review is focused on the description of the post-receptor inhibitors of the GHR-JAK2-STAT pathway, which is one of the most important pathways in the transduction of the somatotropic axis signal. The aim of this review is the short characterization of the main post-receptor inhibitors, such as: cytokine-inducible SH2-containing protein (CIS), Suppressors of Cytokine Signaling (SOCS) 1, 2 and 3, sirtuin 1 (SIRT1), protein inhibitors of activated STAT (PIAS) 1, 3 and PIAS4, protein tyrosine phosphatases (PTP) 1B and H1, Src homology 2 (SH2) domain containing protein tyrosine phosphatase (SHP) 1, 2 and signal regulatory protein (SIRP) α1. The equilibrium between these regulators activity and inhibition is of special concern because, as many studies showed, even slight imbalance may disrupt the GH activity causing serious diseases. The regulation of the described inhibitors expression and activity may be a point of interest for pharmaceutical industry.
Receptor Activator of Nuclear Factor Kappa B (RANK) and Clinicopathological Variables in Endometrial Cancer: A Study at Protein and Gene Level
Authors: Gómez Raúl , Castro Ana , Martínez Jessica , Rodríguez-García Víctor , Burgués Octavio , Tarín Juan J. , Cano Antonio
Published: June 2018

The system integrated by the receptor activator of nuclear factor kappa B (RANK) and its ligand, RANKL, modulates the role of hormones in the genesis and progression of breast tumors. We investigated whether the expression of RANK was related with clinicopathological features of primary endometrial tumors. Immunohistochemistry was used in an endometrial cancer tissue array containing samples from 36 tumors. The amount of RANK mRNA was examined in a tissue scan cDNA array containing cDNA from 40 tumors. Normal endometrium was examined for comparison. Immunohistochemical analyses showed that RANK expression was higher in malignant than in normal endometrium (p < 0.05). RANK expression was related to histological grade (Pearson correlation index = 0.484, p < 0.001), but not to tumor stage or to age of the women. The gene expression was similar in malignant and normal endometrium. The study of RANK isoforms confirmed that the overall relative abundance of the three clearly identified transcripts was similar in normal and pathological endometrium. RANK protein expression increased from normal to malignant endometrium, and the expression level was related with tumor grade but not with stage or the age of subjects in endometrial cancer. In contrast, similar comparisons showed no change in RANK gene expression.
A Community Network Ontology for Participatory Collaboration Mapping: Towards Collective Impact
Authors: De Moor Aldo
Published: June 2018

Addressing societal wicked problems requires collaboration across many different community networks. In order for community networks to scale up their collaboration and increase their collective impact, they require a process of inter-communal sensemaking. One way to catalyze that process is by participatory collaboration mapping. In earlier work, we presented the CommunitySensor methodology for participatory mapping and sensemaking within communities. In this article, we extend this approach by introducing a community network ontology that can be used to define a customized mapping language to make sense across communities. We explore what ontologies are and how our community network ontology is developed using a participatory ontology evolution approach. We present the community network conceptual model at the heart of the ontology. We show how it classifies element and connection types derived from an analysis of 17 participatory mapping cases, and how this classification can be used in characterizing and tailoring the mapping language required by a specific community network. To illustrate the application of the community network ontology in practice, we apply it to a case of participatory collaboration mapping for global and national agricultural field building. We end the article with a discussion and conclusions.
Sustainable Materials for Transportation Infrastructures: Comparison of Three Commercially-Available Metakaolin Products in Binary Cementitious Systems
Authors: Sullivan Matthew S. , Chorzepa Mi G. , Hamid Hiwa , Durham Stephan A. , Kim S. Sonny
Published: June 2018

Metakaolin is the only major natural pozzolan to be specified for use as a supplementary cementitious material in the United States. As a result, the metakaolin market for concrete has grown dramatically in the past 20 years. As of now, the specifications of up to 16 state departments of transportation allow for the use of commercially-available and high-reactivity metakaolin products. However, to the best of the authors’ knowledge, no study has been performed to evaluate whether these products are comparable in their performance. Three commercially-available (U.S.) metakaolin products, each replacing 10%, 15%, and 20% of the cement content in concrete and mortar mixtures are studied. Concrete mixtures contained a cementitious content of 422 kg/m3, a coarse aggregate fraction of 985 kg/m3, and a water-to-cementitious ratio equal to 0.43. Varying levels of a superplasticizer were used to maintain a uniform workability between mixtures. Each mixture was subjected to the following tests: compression, split-cylinder tension, modulus of rupture, dynamic elastic modulus, rapid chloride-ion penetrability, alkali–silica reactivity, sulfate resistance, the coefficient of thermal expansion, and drying shrinkage. Benefits from the inclusion of metakaolin were highly product-dependent and include increases in mechanical strength. All metakaolin supplemented concrete mixtures benefitted from decreased permeability and increased resistance to chemical attacks, with the exception of the sulfate resistance of mortars including a metakaolin product with high fineness. The inclusion of any metakaolin at any replacement level increased the coefficient of thermal expansion of concrete specimens. Reasons for difference in performance between products are discussed, and predictors of quality are recommended.
Critical Analysis of International Precast Concrete Pipe Standards
Authors: Wong Lui S. , Nehdi Moncef L.
Published: June 2018

Reinforced concrete pipe (RCP) standard guidelines and industry practice have rather been disconnected from the end-users’ expectations. No genuine effort has so far been made to create synergy between the expertise gained and advances made around the world. With the advent of strong competition from flexible pipe products and changing end-user expectations, a critical look at the current state-of-the-art is needed. In the present paper, RCP standards from a study area representing a quarter of the world’s population (Canada, United States, United Kingdom, China, Australia and New Zealand) were critically analyzed. Comparisons were made in terms of product and material requirements, structural load testing, hydrostatic performance, and durability requirements. It is shown that the RCP sector lags modern developments in concrete technology, standard code advances and materials innovations. The analysis also revealed various knowledge gaps in terms of the mechanical, hydrostatic and durability performance of RCP. Recommendations emanating from this critical analysis aim at tailoring performance-based guidelines that can better capture current market needs and user expectations.
Sex-Specific Sub-Lethal Effects and Immune Response in Ceratitis capitata Wied. (Diptera: Tephritidae) Challenged with Spinosad
Authors: Mura Maria Elena , Ruiu Luca
Published: June 2018

The main objective of this study was to investigate the effects of the insecticidal compound spinosad on the survival, reproduction, and immune functions of the Mediterranean fruit fly. The lethal and sub-lethal effects were determined on Ceratitis capitata Wied. (Diptera: Tephritidae) challenged with different concentrations of spinosad. A median lethal concentration of 0.28 ppm was observed on flies feeding for 5 days on a treated diet. A significant and concentration-dependent decrease in fecundity, egg hatch rate, and lifespan was also detected in treated compared with control flies. Gene expression analyses conducted on treated insects by RT-qPCR revealed an immunomodulatory action of sub-lethal concentrations of spinosad. Target transcripts included several genes involved in medfly immunity and male or female reproductive functions. While a significant upregulation was detected in treated males a short time after spinosad ingestion, most target genes were downregulated in treated females. Our study confirmed the high toxicity of spinosad to C. capitata, highlighting an indirect effect on insect lifespan and reproductive performance at sub-lethal doses. In addition to defining the acute and sub-lethal toxicity of spinosad against the fly, this study provides new insights on the interaction of this compound with insect physiology.
A Target-Temperature Monitoring System for Cyclotron Targets: Safety Device and Tool to Experimentally Validate Targetry Studies
Authors: Do Carmo Sergio J. C. , De Oliveira Pedro M. , Alves Francisco
Published: June 2018

The present work describes an experimental system enabling temperature measurement in cyclotron targets’ cooling water during bombardment. The developed system provides sensible and immediate response to variations of irradiation conditions during bombardment and enables quantification of the temperature rise in the cooling water due to beam interaction with the irradiated target and with its collimator. Such a system finds application either as a monitoring safety device to instantaneously detect and register abnormal alterations in target conditions to anticipate thermal-related incidents and as a tool to experimentally validate cyclotron targetry optimization studies and thermal simulations.
Oxidized Low-Density Lipoprotein Serum Concentrations and Cardiovascular Morbidity in End Stage of Renal Disease
Authors: Raikou Vaia , Kardalinos Vasilios , Kyriaki Despina
Published: June 2018

Introduction: Oxidized low-density lipoprotein (ox-LDL) is considered a main biomarker of oxidative stress, a common characteristic in end stage renal disease. We examined the relationship between ox-LDL serum concentrations and cardiovascular disease in permanent hemodiafiltration therapy patients. Methods: Ox-LDL values were measured by ELISA and were corrected for LDL-cholesterol (LDL-C) in 96 participants and in 45 healthy control subjects. We performed chi-square tests and adjusted models for the role of ox-LDL on cardiovascular morbidity including coronary artery disease, left ventricular hypertrophy, systolic, diastolic dysfunction and peripheral arterial disease. Results: ox-LDL/LDL-C values were significantly higher in patients than in control group (p = 0.02), due to increased ox-LDL serum levels rather than to low LDL-C. The unadjusted relationship between high ox-LDL/LDL-C and low ejection fraction was found significant (x2 = 9.04, p = 0.003), although the association with the other cardiovascular manifestations was found non-significant. In the adjusted model for the prediction of systolic cardiac dysfunction, high ox-LDL/LDL-C, old age and non-administration of vitamin D supplementation during dialysis session were found to be significant predictors after adjustment to the confounder. Moreover, the association between systolic cardiac dysfunction and non-administration of vitamin D derivatives during dialysis sessions was found significant (x2 = 6.9, p = 0.008). Conclusions: This study showed a significant association between high ox-LDL and systolic cardiac dysfunction in permanent hemodiafiltration therapy patients. This relationship seems to be influenced by aging and pharmaceutical therapy during dialysis sessions, including vitamin D derivatives.
The Impact of Two Different Cold-Extruded Feeds and Feeding Regimens on Zebrafish Survival, Growth and Reproductive Performance
Authors: Monteiro Joana F. , Martins Sandra , Farias Matheus , Costa Telma , Certal Ana Catarina
Published: June 2018

Zebrafish (Danio rerio) is one of the top model organisms used in biomedical research. Therefore, it is fundamental that zebrafish facilities continuously improve husbandry methods to provide fish with the best physiological and welfare conditions that suit each experimental purpose. Nutrition is a husbandry aspect that needs further optimization, as it greatly affects growth, reproduction, health and behaviour. Here, we have compared the impact of different feeding regimens on zebrafish survival, growth and reproductive performance. Mutant and wild-type zebrafish were raised using several combinations of two cold-extruded processed feeds—Skretting®GemmaMicro and Sparos®Zebrafeed—and one live feed (rotifers). Zebrafeed® outperformed GemmaMicro® in terms of survival rate, and embryo viability was also higher when the spawners were fed with Zebrafeed® either from larval stage or upon sexual maturation. In contrast, GemmaMicro® favoured growth, both in size and weight. The use of rotifers until 60 days post-fertilization improved survival of fish co-fed with GemmaMicro®, while delaying their growth. Zebrafeed® performance was not affected by co-feeding rotifers. Overall, we showed that different nutritional formulas affect physiological parameters, allowing for the establishment of feeding protocols adapted to the objectives of each facility. At the same time, we validated Skretting®GemmaMicro and Sparos®Zebrafeed as two commercially available feeds that are well suited for zebrafish nutrition in a laboratory environment.
Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition
Authors: Belin Etienne , Douarre Clément , Gillard Nicolas , Franconi Florence , Rojas-Varela Julio , Chapeau-Blondeau François , Demilly Didier , Adrien Jérôme , Maire Eric , Rousseau David
Published: June 2018

Seed imbibition is a very important process in plant biology by which, thanks to a simple water income, a dry seed may turn into a developing organism. In natural conditions, this process occurs in the soil, e.g., with difficult access for a direct observation. Monitoring the seed imbibition with non-invasive imaging techniques is therefore an important and possibly challenging task if one tries to perform it in natural conditions. In this report, we describe a set of four different imaging techniques that enable to addressing this task either in 3D or in 2D. For each technique, the following items are proposed. A detailed experimental protocol is provided to acquire images of the imbibition process. With the illustration of real data, the significance of the physical quantities measured in terms of their relation to the income of water in the seed is presented. Complete image analysis pipelines are then proposed to extract dynamic information on the imbibition process from such monitoring experiments. A final discussion compares the advantages and current limitations of each technique in addition to elements concerning the associated throughput and cost. These are criteria especially relevant in the field of plant phenotyping where large populations of plants are imaged to produce quantitatively significative traits after image processing.
Special Issue: Candida and Candidiasis
Authors: Nett Jeniel E.
Published: June 2018

This special issue highlights emerging topics related to Candida, the most prevalent fungal pathogen in the hospital setting. The advantages and limitations of new, non-culture based diagnostic techniques are discussed. The issue reviews mammalian and non-mammalian infection models. The manuscripts present updates on several molecular mechanisms of pathogenicity, including filamentation, biofilm formation, and phospholipid production.
Determinants of Stock Market Co-Movements between Pakistan and Asian Emerging Economies
Authors: Aamir Muhammad , Ali Shah Syed Zulfiqar
Published: June 2018

This study analyzes the determinants of stock market co-movement between Pakistan and Asian emerging economies for the period 2001 to 2015. Augmented Dickey and Fuller (ADF) and Philips-Perron (PP) tests are applied to check co-integration between their stock markets. Results of this study reveal that there is long-term integration between the stock market of Pakistan and the stock markets of China, India, Indonesia, Korea, Malaysia and Thailand. This study reports the driving forces of the co-movement between the Pakistan and Asian emerging markets where co-integration is found. Results of the panel data reveal that there are significant underlying forces of integration between Pakistan and each Asian emerging stock market. The findings of this study have significant implications for policy makers in Pakistan who are designing strategies for macroeconomic harmonization and stability of the country’s economy against financial shocks.
Livelihoods on the Edge without a Safety Net: The Case of Smallholder Crop Farming in North-Central Namibia
Authors: Spear Dian , Chappel Angela
Published: June 2018

Semi-arid Namibia is marginal for agricultural production. Low soil fertility combined with low and variable rainfall restrict the livelihoods of smallholder farmers who often struggle to produce enough food. Although historically, communities have adopted a number of coping mechanisms, climate change threatens to further reduce agricultural production. There are many additional options available to smallholder farmers to adapt to climate change, but they are not necessarily adopting these measures despite having noticed increasing temperatures and declining rainfall. Semi-structured interviews were conducted in three villages in Onesi constituency to examine what agricultural practices smallholder crop farmers use, perception of changes in their yields, their perspective on future yields and whether they are planning on changing their agricultural practices. The results suggest that to sustain the livelihoods of rural communities in north-central Namibia, support is needed from local and regional authorities, as well as traditional and religious leaders to assist with enhancing access to information, enabling information sharing on adaptation options, and increasing awareness on climate change, its impacts and what can be done about it. In addition to this, implementation of the adaptation action also requires demonstration sites and building capacity to enable the development of self-help groups.
Bioinformatic Workflows for Generating Complete Plastid Genome Sequences—An Example from Cabomba (Cabombaceae) in the Context of the Phylogenomic Analysis of the Water-Lily Clade
Authors: Gruenstaeudl Michael , Gerschler Nico , Borsch Thomas
Published: June 2018

The sequencing and comparison of plastid genomes are becoming a standard method in plant genomics, and many researchers are using this approach to infer plant phylogenetic relationships. Due to the widespread availability of next-generation sequencing, plastid genome sequences are being generated at breakneck pace. This trend towards massive sequencing of plastid genomes highlights the need for standardized bioinformatic workflows. In particular, documentation and dissemination of the details of genome assembly, annotation, alignment and phylogenetic tree inference are needed, as these processes are highly sensitive to the choice of software and the precise settings used. Here, we present the procedure and results of sequencing, assembling, annotating and quality-checking of three complete plastid genomes of the aquatic plant genus Cabomba as well as subsequent gene alignment and phylogenetic tree inference. We accompany our findings by a detailed description of the bioinformatic workflow employed. Importantly, we share a total of eleven software scripts for each of these bioinformatic processes, enabling other researchers to evaluate and replicate our analyses step by step. The results of our analyses illustrate that the plastid genomes of Cabomba are highly conserved in both structure and gene content.
Control of the Spin Angular Momentum and Orbital Angular Momentum of a Reflected Wave by Multifunctional Graphene Metasurfaces
Authors: Zhang Chen , Deng Li , Zhu Jianfeng , Hong Weijun , Wang Ling , Yang Wenjie , Li Shufang
Published: June 2018

Three kinds of multifunctional graphene metasurfaces based on Pancharatnam–Berry (PB) phase cells are proposed and numerically demonstrated to control a reflected wave’s spin angular momentum (SAM) and orbital angular momentum (OAM) in the terahertz (THz) regime. Each proposed metasurface structure is composed of an array of graphene strips with different deviation angles and a back-grounded quartz substrate. In order to further help readers have a deeper insight into the graphene-based metasurfaces, a detailed design strategy is also provided. With the aid of the designed graphene elements, the proposed metasurfaces can achieve the full 360° range of phase coverage and provide manipulation of SAM and OAM of a circularly polarized (CP) wave at will. More importantly, simultaneous control of these two momentums can also be realized, and in order to demonstrate this function, a THz spin-controlled OAM beam generator with diverse topological charges is created, which can provide one more degree of freedom to improve the channel capability without increasing the bandwidth compared to a linearly polarized (LP) OAM beam. Numerical results verify the proposed graphene metasurfaces, which pave the way for generating spin OAM vortex waves for THz communication systems.
Effects of Ball Milling Processes on the Microstructure and Rheological Properties of Microcrystalline Cellulose as a Sustainable Polymer Additive
Authors: Zheng Yu , Fu Zongqiang , Li Dong , Wu Min
Published: June 2018

To investigate the effect of ball mill treatment of microcrystalline cellulose (MCC) on the rheological properties of MCC-polymer suspension, the structure and physicochemical characteristics of ground samples with different milling time and the rheological behaviors of MCC-starch suspensions were determined and comprehensively analyzed. During the ball milling process, MCC underwent a morphological transformation from rod-like to spherical shape under the combined effect of breakage and an agglomeration regime. The particle size and crystallinity index of MCC exhibited an exponential declining trend with ball milling time. All of the milled MCC samples presented a crystalline cellulose Iβ structure whereas the MCC mechanically treated in a shorter time had better thermal stability. Rheological measurements of starch/MCC suspensions indicated that all the blended paste exhibited shear thinning behavior and ‘weak’ elastic gel-like viscoelastic properties over the whole investigated range owing to the formation of entangled network structure. The rheological behavior of starch/MCC pastes was strongly dependent on milling time and concentration of MCC samples. The increase in milling time of MCC samples resulted in the loss of rheological properties of starch/MCC pastes, where the size of the MCC playing a dominant role in affecting the properties of composite suspension. In addition, a possible network within starch/MCC suspensions was proposed.
Circadian Rhythm and Alzheimer’s Disease
Authors: Homolak Jan , Mudrovčić Monika , Vukić Barbara , Toljan Karlo
Published: June 2018

Alzheimer’s disease (AD) is a neurodegenerative disorder with a growing epidemiological importance characterized by significant disease burden. Sleep-related pathological symptomatology often accompanies AD. The etiology and pathogenesis of disrupted circadian rhythm and AD share common factors, which also opens the perspective of viewing them as a mutually dependent process. This article focuses on the bi-directional relationship between these processes, discussing the pathophysiological links and clinical aspects. Common mechanisms linking both processes include neuroinflammation, neurodegeneration, and circadian rhythm desynchronization. Timely recognition of sleep-specific symptoms as components of AD could lead to an earlier and correct diagnosis with an opportunity of offering treatments at an earlier stage. Likewise, proper sleep hygiene and related treatments ought to be one of the priorities in the management of the patient population affected by AD. This narrative review brings a comprehensive approach to clearly demonstrate the underlying complexities linking AD and circadian rhythm disruption. Most clinical data are based on interventions including melatonin, but larger-scale research is still scarce. Following a pathophysiological reasoning backed by evidence gained from AD models, novel anti-inflammatory treatments and those targeting metabolic alterations in AD might prove useful for normalizing a disrupted circadian rhythm. By restoring it, benefits would be conferred for immunological, metabolic, and behavioral function in an affected individual. On the other hand, a balanced circadian rhythm should provide greater resilience to AD pathogenesis.
Machinability Study of Developed Composite AA6061-ZrO2 and Analysis of Influence of MQL
Authors: James S. Johny , Annamalai A. Raja
Published: June 2018

Aluminium metal matrix replaces high melting point and high density conventional materials, thus minimizing the usage of energy and supporting the environment. This work develops a low-weight, high-strength composite material with the help of AA 6061 and ZrO2 through a stir casting route incorporated with a squeeze casting setup. Machining and machining tools create impacts on clean environments, as they deal with lubricants and power consumption. Having taken this issue into consideration, this research studies the effect of machining parameters on surface roughness, tool wear, and cutting force, while turning the developed metal matrix composite in dry and minimum quantity lubrication conditions. The turning experiment was performed by designing parameters using an L27 orthogonal array. The turning condition was dry and with minimum quantity lubrication (MQL). The responses obtained in the turning process were analysed using the analysis of variance (ANOVA) technique to find the most influential factor and its percentage contribution. Optimal machining parameters were investigated and tabulated with the help of main effect plots and S/N ratio graphs. Studies prove that there is a linear relationship between MQL versus surface roughness and tool wear, and there was no substantial effect on cutting force.
The Effect of Gadolinium on the Microstructures and Charpy Impact Properties of Super Duplex Stainless Steels
Authors: Moon Byung-Moon , Lee Sang-Wook , Kim Moon-Jo , Jung Seung-Rok , Kim Young-Jig , Jung Hyun-Do
Published: June 2018

Super duplex stainless steels (SDSSs), exhibiting excellent strength and corrosion resistance, serve as the attractive materials in a variety of industries. However, improvements in their ductility and impact-toughness are required in extreme environments. In this study, the effects of gadolinium on the microstructures and Charpy impact properties of super duplex stainless steels were investigated. A base super duplex stainless steel (BDSS) and a gadolinium-added super duplex stainless steel (GDSS) were successfully fabricated using an air casting method. The oxygen content and grain size of SDSSs were found to decrease because of high reactivity of gadolinium with oxygen. Moreover, the average inclusion size and area of GDSS also decreased even with a slight decrease in the average distance between inclusions. Both the BDSS and GDSS exhibited typical Charpy impact transition behavior from −196 °C to 200 °C. Moreover, the GDSS impact energies using Charpy test were higher than those of BDSS over the entire temperature range. Moreover, the ductile-to-brittle transition temperature (DBTT) of SDSSs calculated from the fracture appearance transition temperature (FATT) significantly decreased by over 20 °C with the addition of gadolinium.
Friction Determination by Atomic Force Microscopy in Field of Biochemical Science
Authors: Wang Yan , Wang Jianhua
Published: June 2018

Atomic force microscopy (AFM) is an analytical nanotechnology in friction determination between microscale and nanoscale surfaces. AFM has advantages in mechanical measurement, including high sensitivity, resolution, accuracy, and simplicity of operation. This paper will introduce the principles of mechanical measurement by using AFM and reviewing the progress of AFM methods in determining frictions in the field of biochemical science over the past decade. While three friction measurement assays—friction morphology, friction curve and friction process in experimental cases—are mainly introduced, important advances of technology, facilitating future development of AFM are also discussed. In addition to the principles and advances, the authors also give an overview of the shortcomings and restrictions of current AFM methods, and propose potential directions of AFM techniques by combining it with other well-established characterization techniques. AFM methods are expected to see an increase in development and attract wide attention in scientific research.