bluuebunny/crossref_metadata_2025 huggingface dataset:
PENERAPAN HAZARD ANALYSIS CRITICAL CONTROL POINT (HACCP) TUNA (Thunnus sp.) LOIN BEKU DI PT. TRIDAYA ERAMINA BAHARI
Authors: Hasibuan Nirmala Efri , Azka Aulia , Rohaini Anisa Efri
Published: November 2020

PT. Tridaya Eramina Bahari adalah salah satu perusahaan perikanan produsen tuna loin beku di Jakarta. Standar yang ditetapkan untuk menjamin sistem keamanan pangan yaitu HACCP (Hazard Analysis Critical Control Point) harus dipenuhi oleh perusahaan untuk menjamin mutu produk. Tujuan dari penelitian ini yaitu untuk mengetahui penerapan HACCP pada pengolahan tuna loin beku di PT. Tridaya Eramina Bahari. Penelitian ini bersifat deskriptif dengan metode yang digunakan yaitu metode survei dengan menggunakan kuisioner dari responden. Berdasarkan 12 langkah penerapan HACCP di PT. Tridaya Eramina Bahari  meliputi pembentukan tim HACCP, deskripsi produk, identifikasi penggunaan, penyusunan diagram alir proses, pemeriksaan diagram alir proses, analisa bahaya, penetapan Critical Control Point (CCP), penetapan batas kritis, penetapan prosedur monitoring, tindakan koreksi, tindakan verifikasi, serta penetapan dan dokumentasi sudah diterapkan, namun ada tiga tahapan proses yang perlu ditingkatkan keamanan pangannya. Hasil evaluasi penerapan HACCP di PT. Tridaya Eramina Bahari menunjukkan bahwa CCP yaitu pada tahap penerimaan bahan baku, pengemasan dan pelabelan, serta pendeteksian logam.
Comparison of morphogenesis and anatomy between hairy roots and adventitious roots of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.)
Authors: Huong Trinh Thi , Linh Nguyen Thi Nhat , Tung Hoang Thanh , Hien Vu Thi , Luan Vu Quoc , Manh Cuong Do , Tuan Tran Trong , Ngoc Pham Bich , Ha Chu Hoang , Quang Duc Tien Nguyen , Hoang Loc Nguyen , Nhut Duong Tan
Published: November 2020

In this study, morphogenesis and anatomy of adventitious roots and hairy roots of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) were compared. Adventitious roots were derived from four different samples (leaf, petiole, root and callus) in vitro cultured on medium SH supplemented with 5 mg/L IBA. Hairy roots were derived from callus infected with Agrobacterium rhizogenes strain ATCC 15834. The results showed that there were significant differences in morphogenesis and anatomy, the pathway of secondary roots formation, culture medium between adventitous roots and hairy roots. The hairy roots strongly grew and branched on the free-plant growth regulator medium. The secondary root of the hairy root was formed in two pathways: (1) The secondary root formation began at the vesicles system of main root; (2) The secondary root formation was independent of the vesicles system of main root; it kept developing in length and connected to the vesicles system of main root. On the other hand, growing adventitous roots only grew on the environment with exogenous auxin supplementation; also their branching ability were low. The secondary root formation of the adventitous root was started at the vesicles of the main roots and there was only one secondary root that was formed at an location of adventitous root. The results that obtained in this study will be the reference to identify two types of root.
Cloning, expression and enzymatic characterization of recombinant eugenol oxidase (EUGO) in Escherichia coli
Authors: My Binh Pham Thi , Hai Yen Le , Quoc Tuan Tran , Thi Hong Thuong Nguyen
Published: November 2020

Eugenol oxidase (EUGO), a member of the vanillyl alcohol oxidase family, catalyzes the oxidative reaction of vanillyl alcohol to vanillin. This compound is responsible for the vanilla aroma and is widely used as a flavoring agent in food, cosmetics, and pharmaceuticals. Previously, EUGO was cloned and expressed in E. coli TOP10, and purified by anion-exchange chromatography with Q-Sepharose resin but the purification factor was low. To improve the efficiency of the EUGO purification, in this study, we cloned eugo gene into pET-28a vector and expressed it in E. coli Tunetta. The SDS-PAGE analysis of protein extracts obtained from E. coli expressing EUGO under different induction conditions showed that EUGO was expressed mostly in the soluble fraction at 6 hours after induction with 0.1 mM IPTG at 25oC. EUGO was purified by immobilized−metal affinity chromatography with Ni2+-NTA agarose and the in vitro enzymatic activity was characterized. The specific activity of purified EUGO was nearly 4-fold higher than that of the crude enzyme sample. In particular, the enzyme preparation produced by the purification method based on Ni-NTA affinity in this study was 2,5-fold more pure than that produced by Q-sepharose purification method described previously.
Mining and selecting genes encoding chitinase from DNA metagenome data
Authors: Thi Thom Nguyen , Thi Hong Ha Nguyen , Bich Ngoc Pham , Hoang Ha Chu , Bich Hai Ho , Mai Huong Le
Published: November 2020

In this study, genes encoding chitinaseswere obtained by mining the rhizosphere metagenome database of 2-year-old replanted coffee trees in Dak Lak province and annotating using CAZy data. These sequences predictably are covered 90% of chitinase domains by BLASTp and range from 33% to 99% identity in comparison with published sequence data of chitinase genes. In order to find new funtional chitinase gene(s), among 8/56 chitinase genes with sequence identity less than 75%, ChiA_2 sequenceshowed the highest max score andsignificant homology (72%) was initially selected. Analysis of in silico sequence identified a protein motif for a signal peptide of 30 amino acid residues at the N- terminal using SignalP 4.1 server. By InterProscan software, the ChiA_2 gene sequence is predicted to have conserved active site FDGIDIDWE located at 134-142. The ChiA_2 sequence consisting of 1167 nucleotides, encoding for 389 amino acids with a predicted molecular mass of 43 kDa was synthesized, cloned into the pET21 a(+) vector and transformed into E. coli BL21 (DE3) by heat shock. ChiA_2 protein was specifically expressed in the recombinant E. coli BL21 (DE3) cell with an activity of 4.9 U/ml. In this study, analyzing metagenomic data from the soil sample of rhizosphere provided relatively sufficient genetic information of chitinase gene and initially successful expression and functional evaluation of ChiA_2 sequence, encoding Chitinase 2.
Transient expression of gene encoding ZmLEA14A protein in Nicotiana benthamiana plant
Authors: Hong Hanh Ha , Hien Le Thi Thu , Thi Thu Hue Huynh
Published: November 2020

LEA protein family includes proteins accumulated in the late stage of embryogenesis and in vegetative tissues of stress-confronted plant. These proteins have been demontrated to play a major role in plant response to abiotic stresses, such as drought and salinity stress. The genes coding for LEA proteins in maize are divided into 9 groups including LEA 1, LEA 2, LEA 3, LEA 4, LEA 5, LEA 6, SMP, dehydrin, and AtM. The application of LEA genes to improve drought tolerance for plants by genetic engineering has also been studied extensively all over the world. In this study, pCAM/35S-ZmLEA14A-35S vector and pCAM/Ubi-ZmLEA14A-35S vector contained the ZmLEA14A gene isolated from Te vang 1, these vectors were used to transient express into Nicotiana benthamiana tobacco leaves by agro-infiltration method. The results of immunoassay between cmyc specific antibodies with proteins from infected leaves revealed the expression of recombinant ZmLEA14A protein in N. benthamiana leaves. Thereby, two constructs habouring the ZmLEA14A gene work at transcription and translation levels in the model plant that could harnessed for stable transformation in plants.
Improvement of a recombinant β-glucosidase activity of a sequence derived from metagenome database of Binh Chau hotspring
Authors: Thuy Tran Thanh , Nhung Lai Thi Hong , Xuan Le Thi Thanh , Thoa Nguyen Kim
Published: November 2020

Metabolic products obtaining from microorganisms of geothermal ecologies often show special characteristics which help their cells to survive, grow and develop under extreme conditions. Exploiting the microbial gene resource of those environments demands a new approach via uncultured methods. Thanks to the development of metagenomics and bioinformatic softwares, we can exploit novel genes from environment directly. Based on Binh Chau hotspring’s DNA metagenome sequencing, ORF [denovogenes]_32768 encoding  for β-glucosidase is selected for expression into pET17b vector because it shown a low similartity of amino acid sequence as compared to others in Genbank, a high alkali and Tm predicted values. To improve the expression efficiency of β-glucosidase, some factors (host strains, medium culture, IPTG concentration, aeration…) are investigated. The results showed that the recombinant E. coli C43(DE3) reached the highest dried biomass at 8.26 g/L and the maximum enzymatic activity at 0.34 U/mL in shaking condition (TB medium plus 0.25 mM IPTG with the ratio of cultured /flask volume is 20%, 42-48 hours, 30°C). This study demonstrates the capacity of mining a novel gene encoded for enzyme from DNA metagenome of Vietnam hot spring as well as produces recombinant enzyme for biomass conversion.
Cloning, expression and purification of Leptospira LigB antigen in Escherichia coli
Authors: Lan Anh Le Thi , Thi Hang Minh , Thi Thu Hien Nguyen , Thi Ha Giang Pham , Phi Long Trieu , Van Anh Le Thi , Thi Ha Thanh Dao , Thi Thu Thuy Can , Huu Duc Nguyen
Published: November 2020

Leptospira is one of the most common zoonotic diseases in the tropics and subtropics. Humans are infected by exposure to Leptospira contained water or food sources. Leptospirosis usually breaks out after the flood and causes several consequences for people and economy. Leptospirosis disease, if not rapidly detected and treated promptly, it causes serious consequences such as acute hepatitis-kidneys, meningitis and bleeding, heart and nerve complications, and severe illness can lead to death. Therefore, quick and accurate detection of Leptospira pathogen plays a very important role in Leptospirosis disease treatment. Among antigens of Leptospira, a conserved domain of LigB antigen (Leptospiral immunoglobulin-like protein) was reported that is present in the most of pathogenic serovars of Leptospira, but not in the non-pathogenic Leptospira biflexa, thus this conserved domain was used for production of Leptospirosis detection kits as well as vaccine for Leptospirosis. In order to create a kit for Leptospirosis diagonostic, especially detect anti-Leptospira antibodies in Leptospira infected serum and plasma samples, about 1kb gene fragment encoding for conserved domain of LigB (about 36 kb in molecular weight) was used as the material for producing of LigB protein by DNA recombinant technology. In this study, we present the results for cloning, expressing a conserved domain of LigB antigen in E. coli cells and purifying protein by affinity chromatography collumn. The result indicates that recombinant LigB protein was successfully expressed in E. coli Rosetta 1 and purified by Hitrap chealating collumn. The LigB protein concentration after purification reached 60 mg/L medium with 98% purity.  This purified protein will be used as the materials for creating Leptospirosis kit.
Forming active recombinant enterokinase expressed in Escherichia coli
Authors: Thi Thu Hong Le , Kim Phuong Luong , Thi Thu Hien Trinh , Thi Mai Phuong Nguyen , Nam Hai Truong , Thi Huyen Do
Published: November 2020

Enterokinase is a serine protease commonly used in some biotechnology researches. For these purposes, the light chain containing enterokinase activity has usually been expressed as recombinant protein in different expression systems because natural enterokinase extraction is often ineffective. In this study, we examined the formation of recombinant enterokinase expressed in Escherichia coli with biological activity. The thioredoxin-enterokinase (trx-ent) fusion protein was autocleavaged into thioredoxin and enterokinase when expressed under insoluble form, denatured with guanidine and then refolded with suitable oxidation and reduction steps. Meanwhile, soluble expression as well as insoluble form denatured by urea had not enzymatic activity. Denaturant solution of 6 M guanidine along with the re-folding conditions in oxidized glutathione oxidation buffers followed by the reduced glutathione buffer with arginine was applied to produce trx-ent protein capable of self-cleavage. The recombinant light-chain enterokinase protein had a size of about 35 kDa on the Tris-glycine gel. Initial assessment on substance had shown that enterokinase was capable of cleaving thioredoxin-sumoprotease into thioredoxin and sumoprotease. This result provides the base for the production of active recombinant enterokinase to be used in recombinant protein expression technology.
Linoleic acid isomerization ability of Lactobacillus spp. isolated from Vietnamese human intestinal origins
Authors: Xuan Thach Tran , Thi Thu Ha , Thi Hien Vu , The Hung Hoang , Thi Hoa Nguyen , Thu Hong Le Thi , Ngoc Anh Luu Dam , Van Huong Bui , Lan Anh La Thi , Van Quyen Dong , Thi Tuyet Nhung Nguyen
Published: November 2020

Conjugated linoleic acid (CLA) have been shown to exert numerous health benefits, including anti-carcinogenic, anti-atherogenic, anti-diabetic, antiobesity, cholesterol reducing, antioxidant, anti-microbial, immune system modulator and growth-stimulating properties. In human, CLA is produced from Linoleic acid (LA) by gut bacteria. In this study, nineteen Lactobacillus (Lac.) strains isolated from human feces were studied to determine their ability to metabolize LA. The bacteria were grown in the liquid form of anaerobic MRS medium supplemented with 0.5 mg/mL LA. The linoleate isomerase activity in bacteria grown on MRS medium was determined by Gas chromatograpy. The results indicated that 4 out of 19 strains, including strains Lac.02, Lac.05, Lac.14 and Lac.16 are capable of producing about 40-50 μg/mL CLA from LA. Among them, the highest ability to produce CLA from LA is Lac.02 strain. In the production of CLA from LA, enzymes involved in this metabolism in Lactobacillus act as catalysts of hydration/dehydration (CLA-HY), oxidation of hydroxy groups/reduction of oxo groups (CLA-DH), migration of carbon-carbon double bonds (CLA-DC), and saturation of carbon-carbon double bonds (CLA-ER). The cla-dh, cla-dc, cla-hy and cla-er genes that encode enzymes CLA-DH, CLA-DC, and CLA-ER had been found in all Lac.02, Lac.05, Lac.14 and Lac.16 strains. Gas chromatography traces indicated that these strains produced the same compounds, which was subsequently identified as cis-9, trans-11, and trans-10, cis-12 CLA. In the next study, we will optimize the conditions such as substrate concentrations, pH values, temperature and culture time of each strain to obtain the best rerults.
Establishment of a procedure to determine the content of 17 amino acids in soft-shell crab (Scylla sp.)
Authors: Lan Nguyen Phuong , Trung Do Thi Thanh , Vu Van Thu , Thanh Le Tat
Published: November 2020

Mud crab Scylla sp. is a common sea crab species in Vietnam as well as in Asia Pacific. Today, mud crabs are raised on a large scale to be harvested at the soft molting stage because of the high economic value of the finished shell crabs. At present, the processing of soft shell crabs is limited to whole packaging and exporting. However, 30% of soft-shelled crabs in processing often lose their feet and claws, which reduce production costs. Therefore, it is necessary to study the technology of processing soft-shell crabs to improve the value of soft-shelled crab products. Recently, the application of enzymes in processing has brought many benefits such as being environmentally friendly and creating many bioactive substances. In this journal, we built the procedure to determine amino acid content in the processing of Scylla sp. to ensure the quality of products obtained after processing. This procedure based on HPLC using a fluorescence reader. The results showed that the amino acid content after hydrolysis process by enzyme technology reached 65.58% dry weight and contains many valuable amino acids such as lysine, leucine, valine, methionine, histidine.
Study on the expression of interleukins in patients with twin-twin transfusion syndrome
Authors: Duy Anh Nguyen , Thi Sim Nguyen , Huyen Thuong Phan , Thi Phuong Thao Tran , Van Tong Hoang , Duy Bac Nguyen , Thi Lan Anh Luong , Thi Thu Canh Than
Published: November 2020

Twin-twin transfusion syndrome (TTTS) is an unusual and serious condition that occurs in twin pregnancies when identical twins share a placenta but develop discordant amniotic fluid volumes. The TTTS has a high risk of fetal death and birth defects if left untreated. IL-1β, IL-6 and IL-8 have been shown to play an important role in preterm birth in twin pregnancies, which is one of the consequences of TTTS. This study quantified plasma levels of IL-1β, IL-6 and IL-8 in twin pregnant women with (n = 52) and without TTTS (n = 73) by ELISA and analyzed the association of interleukin expression levels with TTTS. The results showed that plasma concentrations of IL-1β and IL-8 were low and not significantly different between the first trimester and the second trimester of pregnancy, between the patients with and without TTTS or between patients with TTTS before and after treatment. IL-6 levels showed no significant difference between first trimester and second trimester of pregnancy or between patients with and without TTTS. Importantly, IL-6 levels were significantly elevated in TTTS patients after surgical treatment compared to those before surgical treatment. In conclusion, IL-6 may be considered as a potential marker for the prognosis of surgical treatment of TTTS.
Whole genome sequencing of the Canine Distemper Virus isolated from dog in 2018
Authors: Thi Roan Do , Duc Thanh Do , Thi Mai Lan Dang , Hong Ngoc Pham , Huu Duc Nguyen , Thi Khue Nguyen , Thi Thu Hien Nguyen , Kim Xuyen Le Thi , Thanh Hoa Le , Thi Thanh Huong Doan
Published: November 2020

The complete nucleotide genome sequence for Canine Distemper Virus strain CDVHN5 isolated from a dog in Hanoi, Vietnam, in 2018, was determined and phylogenetically analyzed. The complete genome size of the CDVHN5 strain was 15,690 nucleotides, encoding for 02 non-structural proteins (C and V protein) and 06 structural proteins (large protein (L), haemagglutinin (H), phosphoprotein (P), nucleocapsid protein (N), fusion protein (F) và matrix protein (M). In the genome, base A accounted for the highest percentage of 30.5%, consisted of 4,798 nucleotides. Base T consisted of 4,177 nucleotides, accounted for 26.62%. Base G consisted of 3,450 nucleotides, accounted for 21.7%. And base C consisted of 3,310 nucleotides, accounted for 21.1%. The percentage of A+T was 57.2%, higher than that of G+C (42.8%). Nucleotide sequencing analysis showed that the CDVHN5 strain had the highest similarity (99.7%) compared with the virulent strain collected in Ho Chi Minh City in 2014 (CDV/dog/HCM/33/140816) that belongs to genotype Asia-1. Comparison to other genotypes, the CDVHN5 strain had a low percentage of nucleotide and amino acid similarities, from 92.4% to 96%, among them, the lowest rate with two strains of vaccine virus Onderstepoort-US-2002 belonging to genotype America-1 and CDV2784-IT-2013 strain of genotype Arctic (92.4%). Phylogenetic analysis showed that the CDVHN5 strain in Hanoi belonged to genotype Asia-1, similar to Chinese, Taiwan, Thailand and Korean strains. To date, the only full-length CDV sequence from Ho Chi Minh City was sequenced and published in Genebank, no data from Northern Vietnam. So that, the addition of whole genome sequence of CDV in Vietnam is essential.
Study on tuberization of Rehmannia glutinosa (Gaertn.) Libosch.
Authors: Hoai Sam Vu , Thi Xuyen Nguyen , Thi Huong Nguyen , Thi Ngoc Anh Duong , Minh Tuyen Nguyen , Duy Phuong Nguyen , Thuy Hien Phan
Published: November 2020

Rehmannia glutinosa (Gaertn.) Libosch. is an herbal plant, which belongs to the Srcophulariaceae family, containing the main active ingredients such as catalpol and verbacoside in its root tubers. In traditional medicine, R. glutinosa tubers have been used in the fresh or dried tuber root or the prepared rehamannia root. They not only possess the comprehensive pharmacological actions in the blood system, endocrine system but also used mainly for anti-tumor treament, immune-enhancement, anti-diabetes, treament for concretion in the urinary tract, etc. R. glutinosa is naturally distributed in China, Korea and Japan. This species was introduced into Viet Nam since 1958 and then planted widely in many the Northern plain and midland provinces. In Vietnam, R. glutinosa produces flowers without seeds, therefore the plant has been mainly propagated by slicing tubers. In this work, we investigated the effect of some plant hormones and sucrose contents on the ability to producing microtubers of R. glutinosa. Experiments were establishmented on the 1/4 MS medium supplemented with 50g/L sucrose.  Results showed that auxin (IBA, NAA) had no effect on the in vitro tuber formation of R. glutinosa. The efficiency of using the single BAP or PP333 was low. The highest in vitro tuberization rate of R. glutinosa obtained on the medium supplemented with the combination of BAP (1.0 mg/L) and PP333  (0.3 mg/L), reached 83.33% and the number of tubers/plant was 5.58. The optimal sucrose concentration for increasing the diameter and weight of microtubers was 70 g/L. 100% of plants with tuber-roots survived in the nursery and thrived on the field
Effect of Panchagavya foliar spray on the plant metabolism and grain yield of Tenai under rainfed condition
Authors: Ananthi K. , Parasuraman P.
Published: November 2020

The crop growth and development are constantly influenced by environmental conditions such as stresses which are the most important yield reducing factors in the world. Millets cultivation is the most important agricultural operation in the country, not only in terms of food security, but also in creating better livelihoods, opportunities for rural population. Water stress and other abiotic stresses can dramatically limit and reduce the plant growth and productivity. In the frame of physiological window, mild drought induces regulation of water loss and uptake in plants allowing maintenance of their leaf relative water content within the limits where the photosynthetic capacity shows no or little changes. But severe drought induces unfavourable changes in plants, leading to inhibition of photosynthesis and growth. The use of bioregulators is the quickest and surest way of boosting crop production under rainfed condition. Panchagavya is an organic product blended from five different cow products, commonly applied to crop plants in organic farming. It is used as foliar spray, soil application and seed treatment. It can act as growth promoter and immunity booster. The organic Panchagavya foliar spray significantly influenced various physiological and biochemical parameters. A field experiment was conducted at Centre of Excellence in Millets, Athiyandal, Tiruvannamalai, during Kharif, 2017 to study the effect of Panchagavya foliar spray for enhancing the productivity of tenai under rainfed situation. In a water shortage conditions, application of enriched FYM +Recommended dose of fertilizer + 3%Panchagavya foliar spray improves plant height, no. of tillers per plant, total dry matter partitioning, SPAD chlorophyll content and highest grain yield (1739.0 kg ha-1) of tenai under rainfed condition.
Studies on correlation and path analysis in germplasm of Indian mustard [Brassica juncea (L.) Czern and Coss.]
Authors: Awasthi Dinesh , Tiwari V.K. , Kandalkar V.S.
Published: November 2020

Studies on correlation revealed that seed yield per plant and harvest index exhibited significant positive correlation and both traits also demonstrated significant positive correlation with days to 50 per cent flowering and number of secondary branches per plant at both genotypic and phenotypic level. This indicated the strong association of seed yield per plant, harvest index, days to 50 per cent flowering and number of secondary branches per plant. Both genotypic and phenotypic correlation co-efficient for seed yield per plant was found significant positive correlation with days to 50 per cent flowering, number of secondary branches per plant, number of siliqua per plant and harvest index. Number of siliqua per plant was found significant positive correlation with number of primary branches per plant, number of secondary branches per plant, number of siliqua per plant and seed yield per plant. Number of secondary branches per plant was found significant positive correlation with days to 50 per cent flowering, number of primary branches per plant, number of siliqua per plant, number of seeds per siliqua, seed yield per plant and harvest index. Further, the path co-efficient analysis showed lowresidual effect (0.00482, 0.00304 and 0.00167) during Rabi 2016-17 and 2017-18 indicating that most of the major yield components were included in the study. Harvest index percentage had the highest direct positive effects on seed yield per plant, number of siliqua per plant, number of seeds per siliqua, number of secondary branches per plant and days to maturity. Based on result it has been concluded that two traits namely seed yield per plant and harvest index exhibited significant positive correlation as well as direct effect on seed yield may be considered for selection and to improve the seed yield of the mustard germplasm.
Effect of integrated nitrogen management on quality of rice
Authors: Mrudhula K. Anny , Veni B. Krishna
Published: November 2020

A field experiment was conducted during Kharif, 2012 at Rice Research Unit, Bapatla to study the quality of rice affected by integrated use of nitrogen. The experiment was laidout in Randomized Block Design with ten treatments. Results of the investigation revealed that significantly the highest quality parameters of rice like head rice recovery, volume expansion ratio, iron and zinc content were recorded by the application of 50 per cent nitrogen though organics and 50 per cent nitrogen through in-organics and it was on par with 100 per cent RDN through organic source treatment.
Correlation studies for seed yield and yield attributes in soybean [Glycine max (L.) Merrill]
Authors: Chavan P.S. , Kalpande H.V. , Kalyankar S.V. , Borgaonkar S.B.
Published: November 2020

An investigation entitled as “Correlation for yield and yield contributing traits in soybean [Glycine max (L).Merrill.] was conducted during Kharif 2018 at Instructional Farm, Department of Agriculture Botany, Vasantrao Naik Marathwada Agriculture University, Parbhani. The experiment was laid out in Randomized Block Design with three replications and seven treatments viz., T1-untreated seeds,T2-Polymer coating, T3-T2+Vitavax, T4-T3+GA3 100ppm, T5-T3+CCC 100ppm, T6-T3+, NAA 50ppm, T7- T3+IAA 50ppm. Seed yield is a complex character and is dependent on number of component characters. Therefore, study of relationship of characters with each other and with seed yield become more important in crop improvement programme. Therefore, it is essential to find out relative contribution of each of the component character with yield. In present study yield and yield contributing character of seed yield was positive and significant correlated with field plant height, number of branches per plant, chlorophyll content, leaf area, germination per cent, moisture per cent, total dry weight, 100 seed weight, oil content, protein content, harvest index and no. of seeds per pod. The results also obtained from this study days to 50 per cent flowering and days to harvest are negative significant to other yield and yield parameter.
A Review: Bacterial Diversity, Physicochemical Factors and Quality of Compost for White Button Mushroom Cultivation
Authors: SINHA Satish Kumar , UPADHYAY Tarun Kumar , SHARMA Sushil Kumar , SHARMA Manju
Published: November 2020

Composting is an aerobic biotic decomposition route that is governed by physicochemical and microbiological factors. The importance of bacterial flora throughout composting is well recognized. Though, diversity of the bacterial population during composting may differ with the composting material and nutrients content. Therefore, it is essential to study the different types of bacteria during the composting of various agricultural byproducts. The aim of this review is to evaluate the diversity of bacteria, physicochemical factors and quality of compost for cultivation of Agaricus bisporus (white button mushroom) for enhancement of better productivity.
Assessment of the Distribution and Characteristics of the Oldest Forest Stand from the Romanian’s Western Plain
Authors: DINCĂ Lucian , BRATU Iulian
Published: November 2020

The present study analysed the stand of the main forest formations from West Plain based on their composition, age classes, diameters, heights, and the spatial distribution. The stands’ analysis did not limit to the study of the distribution of forest species, but also covers aspects regarding the flora, climate and soil. In short, the study presents both the forest station as well as the forest type. The analysed stands belong to the period 1995-2008, from 121608 ha located in the West Plain. The stands are managed by 13 Forests Districts and contain 151 stand elements older than 120 years.
Study Regarding Growing and Development Stages at Soybean Genotypes
Authors: NEGREA Adrian , RUSU Teodor , REZI Raluca , URDĂ Camelia , SUCIU Vasilena
Published: November 2020

Soybean is one of the most important sources of vegetable oil and protein. The aim of this study is to evaluate the behavior of 75 European soybean genotypes from four maturity groups regarding the influence of climatic conditions of 2020 on soybean main growing and development stages. The experiment was conducted at the ARDS Turda in 2020, using a complete randomized block design with three replications. Dates of emergence (VE) and beginning of flowering (R1), were recorded for all 75 soybean cultivars from each of the four maturity groups (000 to I). Results obtained show that growing, and development stages of the studied soybean genotypes are according both with: thermal units and daily period from sunrise to sunset. Effects of daylength and temperature on soybean development were estimated. The rate of development to flowering was fastest for the very early genotypes than for genotypes in the semi-early maturity group (MG).
Wild Herbivore Forage Preferences in Semi-Natural Mountain Hay-Meadows
Authors: GLIGA Adrian , DASCĂLU Dumitrița
Published: November 2020

In the context of increasing grassland abandonment we observed wild herbivore impact on the vegetation in order to understand their influence. To quantify semi-natural grassland usage by wild herbivores, we determined grazing intake in semi-natural hay-meadows surrounded by forest vegetation. Grazing exclusion cages were placed at three locations in the Valea Mare village area situated in Bistriţa-Năsăud County, Romania, from 1 April 2014 – 5 October 2014. Sampling took place at two times during this period, first shortly before the hay meadows were mowed and second at the end of the vegetation growing season. Herbage air dry matter yield was measured using two types of observation plots (cage protected plots and unprotected plots). Plant composition on the studied hay-meadows was analyzed using PC-ORD (in order to explain the preference of herbivores for each location. Although herbage production did not vary significantly between locations, the herbage intake of total dry matter production did vary between locations. Wild animals grazed through the entire growing season and based upon the floristic composition analyses we explained differences in herbage intake based on plant species preference. The management of these meadows maintains an offer of good quality forage throughout summer, and the forest surrounding grasslands provides cover when needed. In order to inhibit woody vegetation and to conserve species-rich open landscape further observation need to be made about the optimal deer species densities.
ANALYTICAL SOLUTION OF THE SCHRÖDINGER INTEGRAL EQUATION
Authors:   Tomsk State University (Tomsk, Russian Federation), BUBENCHIKOV Mikhail A. , BUBENCHIKOV Aleksey M. ,   Tomsk State University (Tomsk, Russian Federation), JAMBAA Soninbayar ,   Mongolian University of Science and Technology;National University of Mongolia (Ulaanbaatar, Mongolia), LUN-FU Alexander V. ,   Gazprom Transgaz Tomsk Ltd. (Tomsk, Russian Federation), CHELNOKOVA Anna S. ,   Tomsk State University (Tomsk, Russian Federation)
Published: November 2020

In this paper, the question about the use of wave dynamics for solving problems of membrane separation of helium isotopes in the gas state at cryogenic temperatures is considered. The dimensionless form of the stationary Schrödinger differential equation is obtained. Following that, the integral representation form of the wave function is written. This form, which is equivalent to the classical equation, is similar to the integral equation with a degenerate core; however, it contains a modulus of the argument with a shift along the real axis. Using the shift operator, the existing exponential function in the Schrödinger integral equation can be split into a differential operator and an exponential function of the argument module which does not contain a shift. The Fourier identity allows reducing the exponent of the modulus of the argument to a regular exponent. Next, based on the general property of a differential operator acting on an exponent, it is possible to calculate the spectral functions of the problem and write down the distribution for the wave function. This distribution is ultimately expressed through the spectra of the potential barrier. Thereafter, the structure and the spectrum of the composite barrier are considered. With the expression determining the reflection coefficient, it is found that the double-barrier system can have a resonant passage of one of the components in the sequence of distances between the layers of the membrane.
NUMERICAL INVESTIGATION OF UNSTEADY GASDYNAMIC PROCESSES AT THE LAUNCH OF SOLID-PROPELLANT ROCKETS
Authors:   Tomsk State University (Tomsk, Russian Federation), KOSTYUSHIN Kirill V.
Published: November 2020

The paper presents the results of the methodology developed for calculating unsteady gasdynamic processes occurring at the launch of missiles, in the gas-dynamic paths of rocket engines, and in the external regions. The method accounts for the variation in the geometry of the solidpropellant charge in the course of solid-propellant rocket engine operation and in the geometry of the computational domain at the rocket launch. The analysis of the unsteady force impact of the supersonic jet on the launch surface is carried out. It is shown that the maximum force action is located in the vicinity of the Mach disks of the unperturbed jet. Numerical studies of gasdynamic processes at the launch of a model solid-propellant booster rocket are implemented including the case when the nozzle plug opening is taken into account. The contribution of the thrust force components at the stage of bootstrap operation is assessed. The presence of the plug at the initial stage of the engine start leads to an abrupt change in the thrust and minor fluctuations, which are damped as the pressure in the combustion chamber rises.
Análise da rede de multibormidades em idosos atendidos em um centro de referência: estudo transversal
Authors: Cerqueira da Silva Manuella Franco , Luz-Santos Cleber , Noronha dos Santos Diana Oliveira , Risso Rattes Tainá Soares , Da Silva Alanna Ribeiro , Miranda Beatriz Santos , Barbosa Jessica Oliveira , Santos Kionna Oliveira Bernardes
Published: November 2020

INTRODUÇÃO: Idosos possuem múltiplos diagnósticos com padrões de similaridade que podem ser analisados pela teoria das redes complexas. Distúrbios crônicos com repercussões na capacidade funcional tendem aumentar em número e gravidade nesses indivíduos, sendo necessária análise integrada para compreender a relação entre envelhecimento e multimorbidades. OBJETIVO: Analisar os padrões de interação entre as doenças que acometem idosos de um centro de referência. MATERIAIS E MÉTODOS: Foram coletados dados clínicos e doenças diagnosticadas em 2973 prontuários de idosos cadastrados de um centro de referência de atenção à saúde do idoso. As doenças foram consideradas nós da rede e os idosos as arestas de modo não direcionada. O peso das arestas correspondiam à quantidade de idosos com duas doenças simultaneamente. Através do software Gephi (versão 0.9.2) foram analisados grau de conectividade entre as doenças (grau de entrada), interação das doenças em sugbrupos (Comunity), doenças mais influentes (Hubs) e doenças que atuam como pontes entre duas ou mais comunidades. RESULTADOS: Foram identificados 30 nós (doenças) e 433 arestas com pesos distintos de acordo com a quantidade de idosos, e 3 comunidades onde o grau de conectividade é maior entre si. A Hipertensão Arterial Sistêmica (HAS) apresentou-se como o Hub de maior influência na rede. CONCLUSÃO: A construção da rede complexa mapeou as doenças e a influência na assistência ao idoso. Informações relevantes para os gestores locais, na busca de intervenções efetivas no serviço que visem contribuir para prevenção das principais comorbidades e auxiliar na independência funcional dos pacientes.
A prevalência da incontinência urinária em mulheres praticantes de exercícios físicos de alto impacto
Authors: Mesquita Vanessa Cordeiro , Aragão Mona Indianara da Costa , Correia Sabrina Amorim , Pereira Amanda Silva , Gomes Sylvia Christina Leite , Azevedo Kryzia de Moraes , Lima Williams Silva , Dias Sávia Francisca Lopes
Published: November 2020

INTRODUÇÃO: A IU atinge cerca de 10 milhões de brasileiros de todas as idades, sendo duas vezes mais comum no sexo feminino, de acordo com a Sociedade Brasileira de Urologia (SBU). Considerada qualquer perda involuntária de urina de acordo com a ICS, podendo ser classificadas em IUU, IUE e IUM. O tratamento para a IU pode ser cirúrgico, farmacológico ou fisioterapêutico. OBJETIVO: Identificar a prevalência de incontinência urinária em mulheres praticantes de exercício físico de alto impacto, em comparação com mulheres que não praticam atividade física. MATERIAIS E MÉTODOS: O estudo foi do tipo transversal, onde se realizou um estudo de caso, sobre a perda involuntária de urina por mulheres, durante o exercício físico de impacto. As participantes foram divididas dois grupos: Grupo 1, mulheres praticantes de exercícios físicos de alto impacto e Grupo 2, mulheres não praticantes de exercícios físicos. As voluntárias realizaram o Pad Test de uma hora, padronizado pela ICS. Além disso, todas responderam o questionário Internacional Consultation on Incontinence Questionnaire – Short Form (ICIQ-SF). RESULTADOS: Houve a presença de sintomas de IUE em ambos os grupos investigados. Entretanto, foi possível comprovar que no grupo onde as mulheres praticavam exercícios de alto impacto a perda de urina foi bem maior em quantidade e em qualidade, observando-se resultados superiores a 60% nas praticantes de exercícios de alto impacto. CONCLUSÃO: As mulheres praticantes de exercícios de alto impacto apresentaram maior perda urinária comprovada pelo Pad Test e uma maior interferência desses sintomas urinários na qualidade de vida comprovada pelo ICIQ-SF.
Segurança e aplicação da plataforma vibratória em pacientes hospitalizados: uma revisão sistemática
Authors: Ferreira de Moura Renata , Carlos Nery dos Santos Alan , Martins Barbosa Ramon , Prata Martinez Bruno , Afonso Gomes Vinícius
Published: November 2020

INTRODUÇÃO: Um curto período de hospitalização, se acompanhado de imobilidade, pode ser capaz de promover declínio das funções musculoesqueléticas, gerando impacto negativo na funcionalidade e qualidade de vida. Com isso, tem-se tornado cada vez mais comum a adoção de estratégias terapêuticas como o uso da plataforma vibratória (PV). OBJETIVO: Sistematizar evidências sobre a funcionalidade e a segurança da aplicação da PV em pacientes adultos hospitalizados. MÉTODOS: Revisão sistemática, registrada na PROSPERO com código CRD42019119672. Desenvolvida nas bases: LILACS, SciELO, MedLine/PubMed, EBSCOhost e PEDro. Descritores e palavras-chave: “Whole body vibration”, “Intensive Care Units”, “hospitalization”, “muscle strenght”, e “functional capacity”. Incluídos estudos que analisaram os efeitos e a segurança da aplicação da PV em pacientes adultos hospitalizados. A qualidade metodológica foi avaliada através da escala Downs and Black. RESULTADOS: Incluídos 2 estudos, um ensaio clínico randomizado e outro estudo de intervenção controlado. A amostra variou entre 24 e 40 sujeitos, de ambos os sexos, média de idade 52±4 anos, com diagnóstico de DPOC e condições variadas. Houve uma melhora na distância percorrida no teste de caminhada de seis minutos e diminuição no tempo do teste de sentar e levantar, aumento dos níveis de irisina e melhora na qualidade de vida, em relação aos parâmetros dos sinais vitais não teve alterações significativas. O escore metodológico foi em média 16. CONCLUSÃO: Os resultados indicam que a PV parece ser viável e segura, podendo trazer efeitos favoráveis na funcionalidade para o tratamento em pacientes adultos hospitalizados, sendo uma alternativa para a reabilitação de forma precoce.
Efeitos da atividade física na histomorfometria muscular esquelética de modelo tumoral murino de caquexia
Authors: De Oliveira Tayrine Resende , Nunes Lopes João Vitor , David Iaggo Raphael , Mansur Machado Frederico Sander , Rocha Alves Mariana , Batista de Paula Alfredo Mauricio , Monteiro Júnior Renato Sobral , Dias Rodrigues Vinicius
Published: November 2020

INTRODUÇÃO: A caquexia associada ao câncer (CAC) é uma síndrome paraneoplásica que ocorre com indivíduos com câncer que é caracterizada pela diminuição gradual de tecidos muscular esquelética e de tecido adiposo, promovendo um quadro de consumação física do indivíduo. A ocorrência de CAC determina de forma significativa a uma pior qualidade de vida e de sobrevida de indivíduos com câncer. A CAC não pode ser revertida pelo amparo nutricional convencional. Tratamentos não-farmacológicos empregados para a CAC tem reportado a realização de atividade física para possibilitar adaptações neurais e de hipertrofia muscular em indivíduos com a síndrome. OBJETIVO: Verificar os efeitos da atividade física em ambiente enriquecido na histomorfometria da musculatura esquelética de camundongos C57BL/6 submetidos ao modelo tumoral murino de caquexia associada ao câncer (CAC) MÉTODOS: Foram utilizados 38 camundongos C57BL/6 fêmeas, distribuídos aleatoriamente nos grupos de estudo. Todos os animais foram submetidos ao modelo tumoral murino singênico de Melanoma Cutâneo (MTMSMC) com a inoculação subcutânea de células B16F10. Os grupos de estudo são animais do experimento diagnostico CRC (n = 12), animais sedentários (n = 11) e animais submetidos à prática de atividade física em ambiente enriquecido (n = 15). O seguimento do estudo ocorreu por um período de dez dias. Após esse momento, todos os animais foram sacrificados e amostras de tecido muscular esquelético do gastrocnêmio foram submetidas às análises histomorfométricas. RESULTADOS: Os resultados da análise inferencial do peso absoluto e relativo muscular esquelético não diferiram entre os grupos do estudo. Todas as comparações das variáveis apresentaram tamanho do efeito pequeno. A análise histomorfométrica muscular revelou que a área da fibra muscular não diferiu entre os grupos do estudo. Contudo, essa área muscular, apresentou tamanho do efeito pequeno. CONCLUSÃO: Os resultados apresentados mostraram que a realização de atividade física no ambiente enriquecido não influenciou na área da fibra do músculo do gastrocnêmio de camundongos C57BL/6 submetidos ao MTMSMC, associada à CAC.
Atuação do fisioterapeuta nos núcleos de apoio à saúde da família em Teresina, Piauí
Authors: Da Silva Allan Dellon , Nogueira Luciana Tolstenko , Silva Hengrid Graciely Nascimento , Frota Samanta Cris Monteiro
Published: November 2020

INTRODUÇÃO: A atuação do fisioterapeuta vai muito além do reabilitar, pois este está inserido em todo o processo de produção de cuidado, dessa forma, desempenha um importante papel na atenção básica.  OBJETIVO: Analisar a atuação do fisioterapeuta nos Núcleos de Apoio à Saúde da Família em Teresina, Piauí. MATERIAIS E MÉTODOS: Trata-se de um estudo descritivo, com delineamento transversal e abordagem quantitativa. Com aprovação pelo Comitê de Ética em Pesquisa, a coleta de dados se deu por meio da aplicação de um questionário semiestruturado com todos os fisioterapeutas que trabalham no NASF-AB de Teresina-PI. RESULTADOS: Todos os profissionais realizam atendimentos individuais na Unidade Básica de Saúde, e as disfunções mais comumente encontradas estão relacionadas às áreas de Traumato-ortopedia (100%), neurologia (83,33%), reumatologia (50%) e fisioterapia respiratória (33,33%). A maioria dos fisioterapeutas realizam atividades em grupo, ações preventivas e educação em saúde voltada para saúde da criança e adolescentes, saúde da mulher, saúde do homem e saúde do idoso. 100% dos profissionais afirmaram realizar acompanhamentos domiciliares, dentre as atividades mais comuns destacam-se as orientações para pacientes e familiares, prescrição de dispositivos auxiliares de marcha e prescrição de exercícios domiciliares. Além disso, a maioria dos participantes mostrou-se insatisfeito quanto à interdisciplinaridade e interação entre as equipes. CONCLUSÃO: Conclui-se que os profissionais realizam atendimentos tanto individual, como de forma coletiva e os atendimentos ocorrem tanto na unidade básica de saúde como no domicilio. No entanto, ainda é comum relacionarem suas atividades à prática clinica e assistencialista.
Effects of physiotherapy on female urinary incontinence
Authors: Cavenaghi Simone , Lombardi Bruna da Silva , Bataus Sandy Carolina , Machado Beatriz Pereira Barbosa
Published: November 2020

INTRODUCTION: Urinary incontinence is defined as any involuntary loss of urine, with females being the most affected. It is classified into three types: stress urinary incontinence; urgency; and mixed. Physiotherapy in the treatment of urinary incontinence consists of normalization of the tone of the pelvic floor muscles, using kinesiotherapy and transcutaneous electrostimulation of the posterior tibial nerve. OBJECTIVE: To evaluate the effects of physiotherapy on female urinary incontinence. METHODOLOGY: It was a clinical, longitudinal and prospective study. The study included 27 women with a mean age of 57.4 years, diagnosed with urinary incontinence, referred for treatment at the School Physiotherapy Outpatient Clinic. They answered a questionnaire to assess demographic and clinical data and the ICIQ-FS quality of life questionnaire, before and after physiotherapeutic intervention through kinesiotherapy and posterior tibial electrostimulation. RESULTS: Most women had stress urinary incontinence (55.6%), and 33.3% (n=9) reported urinary losses from 01 to 04 years and 33.3% (n=9) from 04 to 08 years. The frequency of urinary losses before treatment in the majority (55.6%) was several times a day and after treatment the majority (55.6%) lost once a week or less. There was a significant decrease in the comparison of the ICIQ Score before and after physical therapy treatment (p< 0.0001). CONCLUSION: Physiotherapy, through kinesiotherapy and electrostimulation, is effective in the treatment of female urinary incontinence.