bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlation between Cell Proliferation with Cervical Lymphoid Node Status in Nasopharyngeal Carcinoma Patients
Authors: Nugroho Puguh Setyo , Yusuf Muhtarum , Hidayati Titiek Ahadiyah
Published: April 2021

Several studies showed that the index of nasopharyngeal carcinoma (NPC) cell growth could be used to assess the carcinogenesis interaction factor, development and prognosis of NPC. Cell proliferation index could always be assessed with Ki-67 protein expression test. This research was conducted to study the correlation between cell proliferation index with cervical lymphoid node status in NPC in clinical manifestation to asses the progressivity and prognosis on NPC patients. This study used cross sectional design. Biopsy tissue specimen were acquired from 35 NPC patients clinically divided into four criteria of cervical lymphoid node status (N0, N1, N2 and N3). Expression of Ki-67 protein was acquired by immunohistochemistry test using monoclonal rabbit antibody anti-human Ki-67 clone 901-325-091911 (Biocare Medical, LCC. 4040 Pike Line, CA 94520 USA). The measurement of Ki-67 protein was conducted by pathology consultant. Spearman statistic test was performed to asses the correlation between Ki-67 protein expression and cervical lymphoid node status. The statistical significance was defined as p<0.05. Positive expression of Ki-67 protein was found in 33 patients; 4 patients with N0 (11.43%), 5 patients with N1 (14.29%), 9 patients with N2 (25.71%), and 15 patients with N3. Negative expression of Ki-67 protein was found in 2 patients with N0 (5.71%). The Spearman test resulted at p=0.0001 with correlation coefficient of 0.758. The correlation between Ki-67 protein expression with cervical lymphoid node resulted in a significant correlation (p<0.05). In conclusion, cell proliferation index has correlation with cervical lymphoid node status in NPC patients.
The Big Move: Five Mass Transport To Ankara City Hospital
Authors: BEKGÖZ Burak , ŞAN İshak , ERGİN Mehmet , SARI Eyüp , USUL Eren
Published: April 2021

Aim

It is a rare need for IHT that an existing hospital is scheduled to be closed. In this study, the process of Mass Transport due to the relocation of five different hospitals to a single target hospital is discussed.

Methods

Meetings were held with hospital administrators to plan the Mass Transport and an emergency transport action plan was decided. During the planning phase of the Mass Transport, each hospital was examined separately and in-hospital and inter-hospital transfer routes were created. During the Mass Transport, spare ambulances were activated to avoid disrupting the daily workflow of emergency medical services (EMS). In addition, EMS administrations of nearby provinces were asked to send their spare ambulances to Ankara. While 1 driver and 2 paramedics were assigned to a stable patient transport team, 1 driver, 1 paramedic and 1 doctor were assigned to a critical patient transport team.

Results

The patients who were transported from the clinics comprised 24.95% (n = 130) of all transported patients. Of all patients transported, 56.81% (n = 296) were males. The mean age of all transported patients was 46.33 ± 26.04 years (min-max; 0 - 93) (46.21 ± 26.45 years for females, 46.43 ± 25.56 years for males).

 

Conclusion

Pre-transport planning and measures are crucial for the safe transport of patients, especially in large-scale IHTs. In addition, an effective communication system ensures a trouble-free transport.
SIKAP MASYARAKAT TERHADAP ALIH FUNGSI LAHAN RUANG TERBUKA HIJAU (RTH) MENJADI AREAL PERTAMBANGAN BATU
Authors: Naqiyya Tsabita , Kaskoyo Hari , Qurniati Rommy
Published: April 2021

Global warming is a problem that must be faced by living things. To reduce global warming by providing Green Open Space (GOS). GOS in the city of Bandar Lampung has experienced a broad decline due to the rampant land conversion as happened at Bukit Sukamenanti, due to the existence of rock mining. The purpose of this study was to determine the attitudes of the community, the relationship between the level of knowledge and attitudes and willingness to pay the community to maintain open green space. The sampling method used was simple random sampling with a total of 93 respondents consisting of 35 miners and 58 non-miners. Data collection uses observation, interview and literature studies. Data analysis using quantitative descriptive analysis techniques. The results of the study is the attitude of the community refused to change land use as much as 66.5%. The level of knowledge has a very significant positive relationship to the attitude of the non mining community, but has a non-significant negative relationship between the level of knowledge and the attitude of the mining community. The higher the level of knowledge the community is not the miner, the more positive his attitude will be. But for the mining community, even though they have a high level of knowledge, they do not have an influence on attitudes because of the economic needs that must be met from mining. Total value of willingness to pay the community to maintain green open space is IDR 89.185.648/month.
HABITAT DAN PERILAKU BURUNG JULANG IRIAN (Rhyticeros plicatus) DI RESORT MASIHULAN SEKSI WILAYAH 1 TAMAN NASIONAL MANUSELA
Authors: Sia Marni Yanti , Pattinasarany Cornelis K. , Tuhumury Andri
Published: April 2021

The purpose of this study was to determine the habitat and daily behavior of hornbills at Masihulan Resort, Manusela National Park. Study was conducted on March 2019. Ad libitum sampling method was used to observe the hornbills behavior, while combination of lines and line terraced was used to obtained vegetation data. Hornbills require a natural habitats that are not affected by human activity, but can also adapt to habitats that have been disturbed with low levels of interference. An important factor of habitat that affects the distribution and daily activities of hornbills are the presence of feeding trees and nesting trees. Diet sources of hornbills in both locations consist of five spesies of plants, namely Ficus benjamina, Octomeles sumatrana, Myristica lancifolia, Pometia pinatta, and Eugenia sp; they were more abundant on Ilie than Hua Hui Jalang. The behaviors that had been identified were feeding, calling, grooming, and beak rubbing behavior. Four behaviors were observed at Hua Hui Jalang, namely: feeding (8%), grooming (32%), beak rubbing (40%), and calling (20%); and threebehaviors were observed at Ilie, namely : feeding (29,17%), grooming (31.25%), and calling (39.58%).
KERAGAMAN SUDUT PERCABANGAN DAN PANJANG INTERNODUS TANAMAN UJI KETURUNAN PULAI GADING (Alstonia scholaris (L.). R.Br.)
Authors: Mashudi Mashudi
Published: April 2021

Alstonia scholaris is a local species, widely used for making hadicraft and pencil slate. The purpose of this paper is to determine the effect of provenance and family on the diversity of tree branching angle and internodus length of A. scholaris plants. The experimental design used was a Completely Randomized Block Design with provenance and family treatments. The number of provenance involved was 5 provenances (Lombok, Jayapura, Solok, Timor and Bali) and the number of family involved was 48 families. Each family planted 4 seeds per plot with a spacing of 4 x 2 m and was repeated 3 times. The results of the analysis of variance showed that the provenance and family had a significant effect on the tree branching angle and length of the internodus. The average of tree branching angle between families ranges from 37.50 - 61.25o  and the length of the internodus ranges from 1.15 - 2.20 cm.  Provenance with the best tree branching angle are occupied by the Timor provenance and the best length of the internodus is occupied by the Lombok provenance. The best tree branching angle is occupied by 20 families with an angle range of 50.91o - 61.250  and the best length of the internodus is occupied by 22 families with a range of 1.82 - 2.20 m.
IMPLEMENTASI KEMITRAAN KEHUTANAN DI KESATUAN PENGELOLAAN HUTAN PRODUKSI WAY TERUSAN
Authors: Wandira Yuni Ayu , Kaskoyo Hari , Febryano Indra Gumay , Yuwono Slamet Budi
Published: April 2021

One of the government policies to increase community participation in forest management is Forestry Partnership.  The purpose of this study was to determines the process of forestry partnership implementation in KPHP Way Terusan.  Data collection used in this research was interviews and then the data is analyzed descriptively. The results showed that the implementation process of forestry partnership was long enough and have a lot of challenges.  The supporting factors in forestry partnership were the existence of high support done by the community towards forestry partnership programs, communities high trust to KPHP Way Terusan and high support by other related parties (stakeholders).  Inhibit factors in forestry partnership were low capabilities of human resources, the problems within farmer groups organization, maintainless communications between government and farmer groups and low community participation.
KAJIAN KARAKTERISTIK BRIKET DARI PENCAMPURAN JERAMI DAN SEKAM PADI DARI LIMBAH PASCA PANEN DI LAHAN GAMBUT
Authors: Rianawati Fonny , Abidin Zainal , Naparin Muhammad
Published: April 2021

This study aims to study the characteristics of briquettes from rice straw and rice husks in the form of moisture content, density, ash content, flight substance content, and heating value with different mixing variations in an effort to overcome the occurrence of deep forest / land fires to control forest or land fires in wetland areas by innovating and utilizing technology of straw and rice husks from post-harvest waste.  The test results showed, the characteristic values are not much different for each mixing variation and are still below the Indonesian National Standard (SNI) No. 1-6235-2000, namely The heating value ranges from 2729.33 - 3492.14 cal/g (SNI ≥ 5000 cal/g), bonded carbon content ranges from 2.3524 -12.5870% (SNI ≥ 77%), ash content ranges from 27.3367 -29.8367% (SNI ≤ 8%) and the moisture content ranges from 18.2130 -28.8903% (SNI ≤ 8%). This is presumably due to the large content of the adhesive used, the pressing and drying processes that cause high water content, because high water content will cause a low calorific value. Low heating value will cause a large amount of ash content which causes a low value of bound carbon. However, overall charcoal briquettes from rice husks and rice straw can be applied to the community as a technological innovation that can be used in the use of post-harvest waste in an effort to control forest and land fires with land processing without burning.
KARBON TERSIMPAN DI TATA GUNA LAHAN SUB-SUB DAS KHILAU DAS SEKAMPUNG
Authors: Ningsih Ratih Rinda , Banuwa Irwan Sukri , - Duryat , Yuwono Slamet Budi
Published: April 2021

The khilau is one of sub-watershed of the sekampung watershed. The area was very important due to its function as catchment area which is also located close to TAHURA (great forest park ) Wan Abdurrachman, there for sub-watershed khilau plays a very strategic role in the preservation of biodiversity and climate change.  The study aims were to analyze the carbon determine the total carbon dioxide uptake and oxygen release in the khilau sub-watershed. The carbon stored was extimated by using allometric equation the biomass tree. The biomass expantion factor was employed estimated of  biomass.  The carbon conten multiplication with the value of the conversion of carbon elements to carbon dioxide, while for the oxigen release was estimated by multiplying the value of carbon squestration by the conversion value of carbon atoms to oxygen based on atomic weight. The result showed that carbon stored in the forest was 141,69 tons/ha, it was greater than agroforetry (75,33 tons/ha), shrubs (24,74 tons/ha), annual plant (11,13 tons/ha) and paddy fields (3,45 tons/ha) with a total area of forest 52,22 ha, agroforestry 457,86 ha, shrub 28,74 ha, annual plant 84,8 ha and paddy fields 2,36 ha.  The total carbon dioxide absorbtion was 159.832,73 tons and oxygen release was 426.220,61 tons.
PERTUMBUHAN ANAKAN KOPI (Coffea arabica Lin.) BERBASIS SISTEM AGROFORESTRI DI HUTAN RAKYAT CIMARIAS SUMEDANG
Authors: Wilujeng Sri , Darliana Ina , Solihat Raizal Fahmi , Rohmat Tatang
Published: April 2021

In the recent years, there is a significant increase on economic value of coffee as trade commodity. On that note, the most frequently used cultivation practices for coffee in Indonesia is coffee agroforestry system in private forest. However, there are still some constraints in the system development due to the traditional farmer limited knowledge of coffee cultivation practices. In this study we conducted preliminary observations on the coffee cultivation practices used by the traditional farmers to determine the steps needed to improve the coffee quality and sustainability. We study the effect of plant-spacing and shading on the growth of juvenile plant of coffee (Coffea arabica Lin.) from Sigarar utang variety. The observation was carried out in private forest of Cimarias village Sumedang district for 1 (one) month. We used the combination of 2,5m x 2,5m and 3m x 3m for plant-spacing with kayu afrika (Meisopsis eminii Engl.) as shade and without shade. This experiment used Completely Randomized Design experiment with 10 replication for each treatment, with juvenile height (cm), stem diameter (mm), and the quantity of productive primary branches as data. Then data were analyzed using Anova, Duncan’s Multiple Range Test (DMRT) 0,05, and Pearson Correlation. From the result, the best growth of juvenile plant of coffee tree was showed on 3m x 3m spacing with shade. However, the growth shown has not reached the potential of the coffee from Sigarar utang variety as supposed to in accordance with the environmental condition of the crop. This analysis also supports the prediction of low-productivity because there is positive correlation between juvenile height and the quantity of primary branches, and between stem diameter and the quantity of primary branches.
PEMETAAN JALUR INTERPRETASI EKOWISATA DI DESA PAHMUNGAN, KABUPATEN PESISIR BARAT
Authors: Akmal Rio Rahmad , Djoko W Gunardi , Santoso Trio
Published: April 2021

Ecotourism usually has an educational value packed in the form of natural beauty and is in the countryside. Lampung has many potential ecotourism, one of which is located in West Coast district. This research was conducted in March 2019 to April 2019 in Pahmungan Village, central Coastal district, West Coast Regency, Lampung province.  The Pekon or the village of Pahmungan has an area of 2,600 ha, Pekon Pahmungan is located at 5 ° S and 103 ° E and is 4 km from the centre of the district government, 32 km from the county capital, and 287 km to the provincial capital. Data collection methods used are methods of surveys, interviews, and GIS while the method of data analysis used is a qualitative descriptive method. The purpose of this research is the development of tourism potentials and make the route of ecotourism Intermap in Pahmungan village. The tools used in this study are GPS (Global Positioning System), Camera, Compass, Laptop, and ArcGis application. The object in this research is the ecotourism object that exists in Pahmungan village that will be obtained through the observation of field surveys and interviews with local communities. The results of this research are reflected in the mapping of tourist interpretation lines in which the mapping is an ecotourism pathway that includes various tours in the village Pahmungan. Ecotourism in the village of Pahmungan is not well managed but there are already several community who began to build the development of tourist destinations especially the waterfall tourism, with the program interpretation programs can support the development of Ecotourism and provide comfort for visitors.
KEANEKARAGAMAN TUMBUHAN PENYUSUN VEGETASI HUTAN LINDUNG BENGKUNAT DI RESOR III KPH UNIT I PESISIR BARAT
Authors: Putri Sesilia Maharani , - Indriyanto
Published: April 2021

Plant diversity in Protection Forest is an important factor that considered to support the sustainability function to protected the area. Plant diversity is a form of the species association that could be the indicator forest vegetation stabillities. This research aims to analyze the plant diversity in Bengkunat Protection Forest.  This research was conducted between March-April 2019 at the 4,470 ha area with the sampling intensity 0.02%.  Data were collected using grid line method that consisted of 34 sample plots. Data were analyz using Shannon Index (H), Richness Index (R) and Evenness Index (E). This study found 92 species at the research area.  Dipterocarpus haseltii was the most dominant species based on Important Value Index (IVI) of 34.1%. While the average for other types of IVI is only 2.9%.  The other dominated species were Polyalthia lateriflora, Dacryodes rostata and Dillenia excelsa.  The forest species diversity index (H) was classified as moderate at 1.7, the Richness index (R) was classified as a high level of 30.4 and the evenness index (E) was classified as a high level of 0.9. Based on this research, Bengkunat Protection Forest diversity has a moderated value with the high diversity potential richness and evenness.
PERAN AGROFORESTRI KARET TERHADAP PENDAPATAN PETANI KARET DI DESA MABURAI PROVINSI KALIMANTAN SELATAN
Authors: Saputra Adi , Itta Daniel , - Asysyifa
Published: April 2021

The research location is in Maburai Village, Murung Pudak District, Tabalong Regency, South Kalimantan Province, rubber land that uses the rubber agroforestry system as the object of research. The purpose of this study was to analyze the role of rubber agroforestry in order to increase the income of rubber farmers. The method used in this study to find respondents is by using the Accidental Sampling method. Based on the results of the research that has been carried out, the role of rubber agroforestry is divided into 2 parts, namely, rubber plants for sale, and intercrops play a role for sale, self-consumption, and taken by land owners. The contribution from intercropping was only 4 people who sold the intercrops in the form of fruit, the largest income from the intercropping was Rp. 12,000,000 with a percentage of 19% of the total income of Rp. 63,200,000. There are two factors that affect the agroforestry system, namely internal factors based on farmers' experience, motivation to carry out the rubber agroforestry system, land area, number of plants other than rubber, and types of plants other than rubber, while external factors are the absence of support by rubber agro-forestry extension agencies in Maburai Village, and community leaders.
Two new species of Ilex (Aquifoliaceae) from Thailand
Authors:   Plant Varieties Protection Office, Department of Agriculture, Bangkok 10900, Thailand, Pruesapan Kanchana , Welzen Peter C. van ,   Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, The Netherlands
Published: April 2021

Two new species of Aquifoliaceae are reported for Thailand. Ilex depressifructu is newly recorded from Umphang District, Tak Province (leaf blades ovate, ovate-oblong or oblong, base obtuse; infructescences solitary dichasia; fruits depressed globose with capitate stigma; pyrenes 5, shallowly-5-sulcate, 6-striate) and Ilex phanganensis (like I. umbellulata, but glabrous, smaller leaves, less veins, diplothyrsoid inflorescences with mainly alternate branches, less pyrenes), is newly recorded from Ko Phagnan Islands, Surat Thani Province. The descriptions and illustrations of the taxa are presented.
Hotel website performance: evidence from a transition country
Authors: Pranić Ljudevit , Garbin Praničević Daniela , Arnerić Josip
Published: April 2021

The Purpose – An evaluation of 197 hotel websites in Croatia was conducted to determine whether hotels in this economically lagging transition country exploit the potentials of the Internet as a marketing tool. Design/Methodology/Approach – A team of 30 trained assistants evaluated the websites using an amended modified Balanced Scorecard (mBSC) approach from the user-friendliness, site attractiveness, marketing effectiveness, and F&B informativeness perspectives. MANOVA was used to test main and interaction effects of hotel size, quality rating, and location on four website performance perspectives. Findings – Although hoteliers recognize the importance of online presence, most are not effectively using websites from the user-friendliness, marketing effectiveness, and F&B perspectives. Hotel quality rating, location, and their two-way interaction are significant in explaining differences in site attractiveness and marketing effectiveness. Hotel size does not explain the differences among the four website perspectives. Originality – In lieu of the rapidly evolving IT and consumer trends, this study provides hoteliers with an updated website assessment tool that can serve as a point of comparison against contemporary e-marketing approaches. Moreover, since tourism website evaluation is an under researched topic in transition economies, this article makes a contribution in the needed direction. The study ends with a discussion of results and implications for hoteliers, policy makers, and scholars.
Mobile technologies and supply chain management - lessons for the hospitality industry
Authors: Car Tomislav , Pilepić Ljubica , Šimunić Mislav
Published: April 2021

Purpose – The purpose of this paper is to define preconditions and integration capabilities of the mobile business in supply chain management (SCM) in the hospitality industry via mobile devices and mobile applications. The aims of this paper are to reflect on what mobile technology has to offer and to draw attention to the role of mobile applications and their use in the SCM. Although mobile technologies are present in almost all areas, they are not used to their full potential. Design – The paper defines the concept of supply chain management in the hospitality industry and provides information about benefits of its successful implementation. Furthermore, this paper highlights the role of mobile technologies and mobile applications in supply chain management in the hospitality industry that bring numerous benefits starting with cost reduction, increased functionality, productivity, efficiency and ending up with satisfied users of logistics products in the hospitality industry. Methodology/approach - Based on an extensive literature review, the authors propose new solutions, such us “Bring Your Own Device” (BYOD) in the field of mobile business. Therefore, this paper attempts to investigate the possibilities offered by mobile technologies in the SCM. Findings – The paper introduces new trends in the field of mobile technology and emphasizes their importance and the impact in the management of modern supply chain. Furthermore, the findings in this paper may be useful in developing mobile solutions for SCM in the hospitality industry. Originality – This article provides useful insights for all participants in supply chain to better understand the benefits of the application of mobile technologies and mobile applications in the management of modern supply chain. The paper can also serve as a basis for further research in the area of application of mobile technologies in creating logistics products in the hospitality industry.
Freehand Drawing and Architectural Expression
Authors: Budiman Hanif , Numan Ibrahim , Idham Noor Cholis
Published: April 2021

The aspect of expression is very important in design ideas, amidst the pressure of programming and procedural thoughts nowadays. The expression is a performance that was born from the deepening of a strong identity that makes us further involvement. Freehand drawing is believed to be an important activity that cannot be separated from an architectural design process. Freehand drawing is proven to be an effort that will increase awareness to produce creative thoughts that remain logical. The natural and organic character of a freehand drawing with its flexibility will be able to express the unique value of each design idea. It is very different when compared to the stereotypical technical line of mechanics. This paper aims to discuss the role of freehand drawing in improving the quality of expression during the design process. The discussion was carried out through a qualitative descriptive analysis based on the experience of carrying out freehand drawing courses and several freehand drawing learning and practices. The topic of the role of freehand drawing in the digital age is very strategic because it reminds us of the importance of the role of balance cognition for the design process in general. Keywords: design; expression; freehand drawing; identity.
Glacial Ripping in Sedimentary Rocks: Loch Eriboll, NW Scotland
Authors: Hall Adrian Malcolm , Mathers Hannah , Krabbendam Maarten
Published: April 2021

Glacial ripping is a newly recognized process sequence in which subglacial erosion is triggered by groundwater overpressure. Investigations in gneiss terrain in lowland Sweden indicate that ripping involves three stages of (i) hydraulic jacking, (ii) rock disruption under subglacial traction and (iii) glacial transport of rock blocks. Evidence for each stage includes, respectively, dilated fractures with sediment fills, disintegrated roches moutonnées and boulder spreads. Here we ask: can glacial ripping also occur in sedimentary rocks, and, if so, what are its effects? The case study area is in hard, thinly bedded, gently dipping Cambrian quartz-arenites at Loch Eriboll, NW Scotland. Field surveys reveal dilated, sediment filled, bedding-parallel fractures, open joints and brecciated zones, interpreted as markers for pervasive, shallow penetration of the quartz-arenite by water at over-pressure. Other features, including disintegrated rock surfaces, boulder spreads and monomict rubble tills, indicate glacial disruption and short distance subglacial transport. The field results, together with published cosmogenic isotope ages, indicate that glacial ripping operated with high impact close to the former ice margin at Loch Eriboll at 17.6-16.5 ka. Glacial ripping thus can operate effectively in bedded, hard sedimentary rocks and the accompanying brecciation is significant – if not dominant - in till formation. Candidate markers for glacial ripping are identified in other sedimentary terrains in former glaciated areas of the Northern Hemisphere.
Provisioning Cybersecurity in ICT Complex Supply Chains: An Overview, Key Issues and a Relevant Architecture
Authors: Masip-Bruin Xavi , Marín-Tordera Eva , Ruiz José , Jukan Admela , Trakadas Panagiotis , Cernivec Ales , Lioy Antonio , López Diego , Santos Henrique , Gonos Antonis , Silva Ana , Soriano Jose , Kalogiannis Grigorios
Published: April 2021

The specific demands inherent to supply chains built upon large IoT systems, make a must the design of a coordinated framework for cyber resilience provisioning intended to guaranteeing trusted supply chains of ICT systems, built upon distributed, dynamic, potentially insecure and heterogeneous ICT infrastructures. As such, the proposed solution is envisioned to deal with the whole supply chain system components, from the IoT ecosystem to the infrastructure connecting them, addressing security and privacy functionalities related to risks and vulnerabilities management, accountability and mitigation strategies as well as security metrics and evidence-based security assurance. In this paper we present FISHY, as a preliminary designed architecture, designed to orchestrate both existing and beyond state-of-the-art security appliances in composed ICT scenarios and also leveraging capabilities of programmable network and IT infrastructure through seamless orchestration and instantiation of novel security services, both in real-time and proactively. The paper also includes a thorough business analysis to go far beyond the technical benefits of a potential FISHY adoption as well as three real-world use cases where to strongly support the envisioned benefits of a FISHY adoption.
Adaptive Online Learning for Time Series Prediction
Authors: Shao Weijia , Radke Lukas Friedemann , Sivrikaya Fikret , Albayrak Sahin
Published: April 2021

We study the problem of predicting time series data using the autoregressive integrated moving average (ARIMA) model in an online manner. Existing algorithms require model selection, which is time consuming and inapt for the setting of online learning. Using adaptive online learning techniques, we develop algorithms for fitting ARIMA models with fewest possible hyperparameters. We analyse the regret bound of the proposed algorithms and examine their performance using experiments on both synthetic and real world datasets
Leafy Stems of Phagnalon saxatile subsp. saxatile from Algeria as a Source of Chlorogenic Acids and Flavonoids with Antioxidant Activity: Characterization and Quantification Using UPLC-DAD-ESI-MSn
Authors: Haddouchi Farah , Chaouche Tarik Mohammed , Ksouri Riadh , Larbat Romain
Published: April 2021

Phagnalon saxatile subsp. saxatile is a wild species very widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, informations are still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems. For this purpose, the effects of four extracting solvents were investigated on the content of phenolic compounds and the antioxidant activity of this plant. Extracts prepared with polar solvents (methanol and water) contained higher amounts of phenolic compounds and showed better antioxidant activity than extracts with apolar solvents (hexane, dichloromethane). The methanolic extract, richest in total phenolic and total flavonoid, had significant antioxidant activity as regarded by DPPH° scavenging capacity (IC50 of 5.47 µg/mL), ABTS scavenging capacity (IC50 of 63.77 µg/mL) and inhibition of oxidation of linoleic acid (IC50 of 22.71 µg/mL), when compared to synthetic antioxidants. Chlorogenic acids and few flavonoids were identified and quantified by UPLC-DAD-MSn. The di-O-caffeoylquinic acids isomers were the most concentrated phenolics (25.4mg/g DW) in the methanolic extract.
Role of alpha-Synuclein in Cell Biology: A Hypothesis
Authors: Minda Renu
Published: April 2021

I wish to suggest a hypothesis regarding the physiological function of alpha-synuclein, a protein implicated in Parkinson disease, whose cell biology is still debated. The essence is the concept of Carnot cycle of refrigeration in which membrane fluidity depends on the heat produced by mitochondria. The hypothesis has the potential to explain the diverse phenotypes attributed to alpha-synuclein under both physiological and pathological conditions.
A Dual-Color Tyr-FISH Method for Visualizing Genes/Markers on Plant Chromosomes to Create Integrated Genetic and Cytogenetic Maps
Authors: Kudryavtseva Natalya , Ermolaev Aleksey , Karlov Gennady , Kirov Ilya , Shigyo Masayoshi , Sato Shusei , Khrustaleva Ludmila
Published: April 2021

In situ imaging of molecular markers on a physical chromosome is an indispensable tool for refin-ing of genetic maps and validation genome assembly at the chromosomal level. Despite tremen-dous progress in genome sequencing the plant genome assembly at chromosome level still remain a challenge. Recently developed optical and Hi-C mapping aim to assist in genome assembly. For high-confidence in the genome assembly at chromosome level more independent approaches will be required. The present study aimed to refined an ultrasensitive Tyr-FISH technique and to de-velop a reliable and easy method for in situ mapping of a short unique DNA sequences on plant chromosomes. We have carefully analyzed the critical steps of the Tyr-FISH technique to find out the reasons for the failures of using the method. It has been shown that successful visualization of marker/gene depends significantly on method of chromosome slide preparation, probe design and labelling, high stringency washing. Appropriate adjustment of these steps allowed us to detect a short DNA sequence of 1.7Kb with a frequency of 51.6%. Based on our results, we developed a reliable and simple protocol for dual-color Tyr-FISH visualization of short unique DNA sequences on plant chromosomes. New protocol allows more accurate determination of the physical distance between markers and can be applied for faster integration of genetic and cytogenetic maps.
Ovarian reserve and endometrial receptivity in patients after organ-sparing surgeries for ovarian endometriotic cysts
Authors:   N.I.Pirogov Russian National Research Medical University, Moscow, Russian Federation, Solomatina A.A. ,   City Clinical Hospital No 31, Moscow Healthcare Department, Moscow, Russian Federation, Mihaleva L.M. ,   City Clinical Hospital No 31, Moscow Healthcare Department, Moscow, Russian Federation,   Research Institute of Human Morphology, Moscow, Russian Federation, Khamzin I.Z. ,   City Clinical Hospital No 31, Moscow Healthcare Department, Moscow, Russian Federation, Bratchikova O.V. ,   N.I.Pirogov Russian National Research Medical University, Moscow, Russian Federation,   City Clinical Hospital No 31, Moscow Healthcare Department, Moscow, Russian Federation, Tyumentseva M.Yu. ,   N.I.Pirogov Russian National Research Medical University, Moscow, Russian Federation,   City Clinical Hospital No 31, Moscow Healthcare Department, Moscow, Russian Federation, Chabieva L.B. ,   N.I.Pirogov Russian National Research Medical University, Moscow, Russian Federation,   City Clinical Hospital No 31, Moscow Healthcare Department, Moscow, Russian Federation, Hovanskaya T.N. ,   City Clinical Hospital No 31, Moscow Healthcare Department, Moscow, Russian Federation,   Research Institute of Human Morphology, Moscow, Russian Federation
Published: April 2021

Objective. To analyze the significance of the ovarian reserve (OR) in the assessment of endometrial receptivity in patients after cystectomy for ovarian endometriotic cysts (OECs). Patients and methods. We examined 172 patients after organ-sparing surgeries for OECs. The mean patient age was 28.7 ± 4.8 years. Group I included 114 women with low OR. Group II comprised 58 patients with unchanged OR. All study participants underwent pelvic ultrasonography in the middle luteal phase 6 and 12 months postoperatively. We evaluated endometrial thickness and its echostructure, as well as Doppler-angle-independent flow indices, including resistance index, pulsatility index, and systolic/diastolic ratio. Reduced OR was diagnosed according to the ESHRE recommendations (2011 Anti-Müllerian hormone ≤ 0.5–1.1 ng/mL and ≤5–7 antral follicles in one ultrasound section. Results. Impairments in the morphological and functional state of the ovaries were associated with changes of the endometrial thickness, its ultrasonic structure, and hemodynamic parameters in the terminal branches of the uterine arteries (hypovascularization, increased flow indices). Simultaneous use of various methods for the assessment of ovarian tissue and endometrium allowed the detection of a decreased reproductive potential. Patients with a reduced OR after surgeries for OECs demonstrated improvement of both morphological/functional characteristics of ovarian tissue and endometrial receptivity, which allows pregnancy planning. Patients with extremely thin endometrium (3.0–5.0 mm) that does not improve are recommended to resort to assisted reproductive technologies. Key words: ovarian reserve, endometrium, ovarian endometriotic cysts
Infertility associated with ovarian endometriosis: a modern view to the problem
Authors:   Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation, Khamoshina M.B. , Orazov M.R. ,   Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation, Abitova M.Z. ,   Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation, Volkova S.V. ,   Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation, Aryutin D.G. ,   Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation, Aleev I.A. ,   Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation,   Moscow City Clinical Hospital named after V.M.Buyanov, Moscow, Russian Federation, Bayramova A.A. ,   Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation
Published: April 2021

The review summarizes current understanding of the pathogenesis of ovarian endometriosis and the mechanisms of formation of infertility associated with ovarian endometriosis, as well as the possibilities of treating ovarian endometriosis and overcoming the associated infertility. Key words: pregnancy, infertility, ovarian reserve, surgical treatment, endometriosis, endometrioid ovarian cysts
VOLDEMORT E BOLSONARO: DIÁLOGO ENTRE ARTE, MÍDIA E POLÍTICA
Authors: Paula Luciane de , Moura Giovana Cristina de
Published: April 2021

O Brasil está regido por um governo marcado por uma tendência totalitária historicamente arquitetada desde o integralismo, com encaminhamento que pode ser entendido como neonazifacista, calcado na intolerância. Na saga Harry Potter (HP), as marcas da política vilanesca de Voldemort refletem e refratam as do atual governo brasileiro. O objetivo deste artigo é pensar sobre as simetrias entre vida e arte, semiotizadas por Voldemort e Bolsonaro, além de verificar como essa correlação se tornou debate na comunidade de fãs de HP, com relação à associação da obra à política. O método adotado é o dialético-dialógico bakhtiniano. As concepções mobilizadas são: diálogo, enunciado, vozes sociais, reflexo e refração, forças centrípetas e centrífugas, arte e vida. Algumas respostas dos fãs de HP a um manifesto de repúdio à candidatura de Bolsonaro à presidência da República em 2018, postado no maior portal da comunidade no Brasil, o Potterish, em cotejo com declarações do Presidente, compõem esta reflexão, dada a repercussão do evento à época. Os resultados confirmam o pressuposto de que a arte é semeada e nutre a vida, ao menos, é o que ocorre entre Voldemort e Bolsonaro, conforme o próprio manifesto do Potterish.
Human adipose derived mesenchymal stem cells differentially produced anti-inflammatory oxylipins under proinflammatory stimulation
Authors: Wang Dan , Shen Zhengze , Yu Chaoqun , Cheng Lifang , Chen Daorong , Yuan Zhang
Published: April 2021

MSCs have been proven to have immune modulation and anti-inflammation capabilities, but the mechanisms are still under investigation. Recently, oxylipins have been identified to be involved in the immuno-regulation function of MSCs. In the present research, we employed a newly developed UPLC-MS/MS method to identify and quantify the oxylipin profile of ADSCs under proinflammatory stimulation (TNF-α and IFN-𝛾). As a result, among the analyzed 71 oxylipins, we detected and quantified 49 oxylipins derived from six major n-6 and n-3 polyunsaturated fatty acids (PUFAs) including arachidonic (AA), linoleic (LA), alpha-linolenic (ALA), dihomo-𝛾-linolenic acid (DGLA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids in all samples, while 4 oxylipins were detected only in a part of samples and 18 were not detected in all samples. Nine oxylipins were found to be significantly differentially produced in ADSCs after 24 hours of inflammatory stimulation, among which, several oxylipins were reported to be having anti-inflammation or proresolving functions and involved in lipid mediator switching. The results reported here make a fundamental step towards a comprehensive characterization of MSCs derived oxylipins as potential modulators of inflammation and immunoreaction.
Genetic analysis of Sperata sarwari (Singharee) population fragmentized by Physical barriers in the Indus drainage system of Punjab, Pakistan.
Authors: Aziz Asma , Jabeen Farhat , Nafees Muhammad , Sehrish Adiba Khan , Ullah Inayat , Mirza Zahid Sharif , Raza Muhammad Ahmad , Ullah Sami , Afshan Noor
Published: April 2021

Physical barriers like head works, dams barrages are main cause of fragmentation and declining of freshwater fish population in natural habitat. Present study focused on RAPD marker technique to assess the genetic variability among and between the populations of endangered sperata sarwari (Singharee) inhabited in the Indus drainage system of Punjab. Total eight populations (80 speciemen) of S. sarwari were collected from the up and downstream of four Rivers (Chenab, Jhelum, Ravi and Indus) of Punjab. Genomic DNA isolated from muscle tissue of adipose side and ten RAPD marker were used, which produced 50 scorable bands with average band ranging from 250–1050 bp which used for further genetic analysis of S. sarwari. Downstream Indus population of S. sarwari showed highest values of observed alleles (na), effected alleles (ne), Nei’s diversity (h), Shannon index (I) and polymorphism which indicated that downstream Indus population was more genetically variant. The genetic variability (0.5124) and genetic flow (0.4758) among the eight population S. sawari was observed. The up and downstream population showed the highest genetic distance (0.5738) and lowest genetic similarities (0.5634) which indicated complete isolation of Ravi population from other six population. Dendrogram showed that up and downstream Ravi population was completely isolated from the other six up and down stream population of S. sarwai. Overall results indicated that the presence of high fragmentation in River Ravi caused the destruction of habitat and decline in population of S. sarwai in the River Ravi.
Heterogeneous node copying from hidden network structure
Authors: McGillivray Max Falkenberg
Published: April 2021

Node copying is an important mechanism for social network formation, yet most models assume uniform copying rules. Motivated by observations of heterogeneous triadic closure in real networks, we introduce the concept of a hidden network model – a generative two-layer model in which an observed network evolves according to the structure of an underlying hidden layer – and apply the framework to a model of heterogeneous copying. Framed in a social context, these two layers may represent a node’s inner social circle, and wider social circle, such that the model can bias copying probabilities towards, or against, a node’s inner circle of friends. Comparing the case of extreme inner circle bias to an equivalent model with uniform copying, we find that heterogeneous copying suppresses the power-law degree distributions commonly seen in copying models, and results in sparse networks with significantly higher clustering than even the most optimum scenario for uniform copying. Similarly large clustering values are found across a range of real collaboration networks, lending empirical support to the mechanism.
Comparison of obesity indices and triglyceride glucose related parameters to predict type 2 diabetes mellitus among normal-weight elderly in China
Authors: KE Pan , WU Xia , XU Minzhi , FENG Jie , XU Hongbin , GAN Yong , WANG Chao , DENG Zhenyu , LIU Xiang , FU Wenning , TIAN Qingfeng , HE Yan , ZHONG Lirong , JIANG Heng , LU Zuxun
Published: April 2021

Purpose: Although a significant proportion of type 2 diabetes mellitus (T2DM) cases arose from normal-weight individuals, studies on indicators that can predict T2DM in normal-weight people are limited. Accordingly, this study aims to investigate the predictive value of obesity indices and triglyceride glucose related parameters (TyG-related parameters) in T2DM among normal-weight Chinese elderly.Methods: A total of 24,215 normal-weight Chinese elderly (age ≥ 60 years) [body mass index--BMI (18.5-23.9 kg/m2)] were included. Obesity indices and triglyceride glucose related parameters (TyG-related parameters) included waist circumference (WC), waist-to-height ratio (WHtR), visceral adiposity index (VAI), lipid accumulation product (LAP), and TyG-related parameters (TyG, TyG-BMI, TyG-WC, and TyG-WHtR). Multivariate logistic regression analysis was performed to examine the associations between obesity-and TyG-related indices and T2DM. The areas under the curve (AUC) of the receiver-operating characteristic (ROC) curve analyses were used to evaluate and compare the predictive value of the different indices. Results: The prevalence of T2DM was 14.2% in normal-weight individuals. Among the indices, TyG was significantly associated with T2DM among men and women, respectively (adjusted odds ratio--aOR 19.005; 95% CI 13.235–27.291) and (aOR 25.262; 95% CI 16.957–37.634). Compared with other indices, TyG had the highest AUC value for T2DM in men (AUC: 0.818, 95% CI 0.810–0.825) and women (AUC: 0.824, 95% CI 0.814–0.833).Conclusions: TyG is an effective marker and outperforms than other indices when predicting T2DM in the normal-weight Chinese elderly population.Level of evidence: Level V
The impact of COVID 19 pandemic on pattern of cancer mortality in Najran, Saudi Arabia: a single-cancer center experience
Authors: Badheeb Ahmed M , Badheeb Mohamed A , Alhakimi Hamdi A
Published: April 2021

Background: The aim of this paper is to compare the patterns and determinants of cancer mortality in Najran region before and after the COVID-19 epidemics. The association between cancer mortality and each of age, sex, site of cancer, stage, and the 30-days survival rate after the last dose of chemotherapy were assessed.Materials & Methods: Adult cancer patients who died of cancer in King Khalid Hospital in Najran Saudi Arabia, were included in this retrospective observational study. We compared mortality patterns in a period of 6 months in 2020 (March to August) with the corresponding period of 2019.Results: 50 dead adult cancer patients were included, 24 in 2019 and 26 in 2020. Among them, 21% vs 42% were younger than 65 years of age; 61% vs 62% were males, for the years 2019 & 2020 respectively. The top three killers in 2019 were colorectal, gastro-esophageal cancers, and hepatocellular carcinoma, while in 2020 were colorectal, hepatocellular carcinoma, and lymphomas. About 16.7% of patients died within 30 days of receiving anti-cancer treatment in 2019 in comparison with 7.7% in 2020. The difference in the 30-days mortality after receiving anti-cancer treatment was not statistically significant between 2019 and 2020 (p = 0.329).Conclusion: The Year 2020, the time of the COVID-19pandemic, was not associated with a significant increase in short-term mortality among patients with malignancy in Najran, Saudi Arabia. Our results generally reflect the crucial role of strict preventive national measures in saving lives and warrants further exploration.