bluuebunny/crossref_metadata_2025 huggingface dataset:
Susceptibility of Anopheles gambiae from Côte d’Ivoire to insecticides used on insecticide-treated nets: evaluating the additional entomological impact of piperonyl butoxide and chlorfenapyr
Authors: Kouassi Bernard L. , Edi Constant , Tia Emmanuel , Konan Lucien Y. , Akré Maurice A. , Koffi Alphonsine A. , Ouattara Allassane F. , Mea Antoine Tanoh , Zinzindohoue Pascal , Kouadio Blaise , Andre McKenzie , Irish Seth R. , Armistead Jennifer , Dengela Dereje , Cissé Ndombour G. , Flatley Cecilia , Chabi Joseph
Published: December 2020

Background: Pyrethroid-treated mosquito nets are currently the mainstay of vector control in Côte d’Ivoire. However, resistance to pyrethroids has been reported across the country, limiting options for insecticide resistance management due to the paucity of alternative insecticides. Two types of insecticide-treated nets (ITNs), ITNs with pyrethroids and the synergist piperonyl butoxide (PBO), and Interceptor®G2 nets, a net treated with a combination of chlorfenapyr and alpha-cypermethrin, are believed to help in the control of pyrethroid-resistant mosquitoes. Methods: The susceptibility of Anopheles gambiae sensu lato (s.l.) to pyrethroid insecticides with and without pre-exposure to PBO as well as to chlorfenapyr was investigated in fifteen sites across the country. Susceptibility tests were conducted on 2- to 4-day old adult female An. gambiae s.l. reared from larval collections. The resistance status, intensity, and effects of PBO on mortality after exposure to different concentrations of deltamethrin, permethrin and alpha-cypermethrin were determined using WHO susceptibility test kits. In the absence of a WHO-recommended standard protocol for chlorfenapyr, two interim doses (100 and 200 µg/bottle) were used to test the susceptibility of mosquitoes using the CDC bottle assay method. Results: Pre-exposure to PBO did not result in full restoration of susceptibility to any of the three pyrethroids for the An. gambiae s.l. populations from any of the sites surveyed. However, PBO pre-exposure did increase mortality for all three pyrethroids, particularly deltamethrin (from 4.4% to 48.9%). Anopheles gambiae s.l. from only one site (Bettie) were susceptible to chlorfenapyr at the dose of 100 µg active ingredient (a.i.)/bottle. At the dose of 200 µg (a.i.)/bottle, susceptibility was only recorded in 10 of the 15 sites.Conclusion: Low mosquito mortality was found for pyrethroids alone, and while PBO increased mortality, it did not restore full susceptibility. The vector was not fully susceptible to chlorfenapyr in one third of the sites tested. However, vector susceptibility to chlorfenapyr seems to be considerably higher than for pyrethroids alone or with PBO. These data should be used cautiously when making ITN procurement decisions, noting that bioassays are conducted in controlled conditions and may not fully represent field efficacy where the host-seeking behaviours, which include free-flying activity are known to enhance pro-insecticide chlorfenapyr intoxication to mosquitoes.
The utility of simultaneous CT-guided localization for multiple pulmonary nodules using microcoil before Video-Assisted Thoracic Surgery
Authors: Xu Yanyan , Ma Lingchuan , Sun Hongliang , Huang Zhenguo , Zhang Zhenrong , Xiao Fei , Ma Qianli , Lin Jie , Xie Sheng
Published: December 2020

Background: To evaluate the feasibility and safety of microcoil in simultaneous localization for multiple pulmonary nodules before video-assisted thoracic surgery (VATS). Methods: Twenty-eight consecutive patients (26 two-nodule, 2 three-nodule; totally 58 nodules; Group A) underwent simultaneous CT-guided localization for multiple pulmonary nodules before VATS using microcoil. Successful targeting, localization, and VATS were defined as implantation of microcoil at the target site on CT image obtained immediately after the marking procedure, visualization of nodule location, and complete resection of the target nodule with adequate margin, respectively. Meanwhile, the clinical characteristics, localization procedure-related variables of the nodules and procedure-related complication in group A were also assessed and compared with those in a control group (221 single-localization procedures in 221 patients; Group B). Results: The similar rates of success targeting, localization and VATS were observed in group A and B (96.6% vs 98.2%; 91.4% vs 91.0%; 100% vs 99.1%). Although the rate of overall complications (including localized pneumothorax and intrapulmonary hemorrhage) was a bit higher in group A than that in group B (32.8% vs 30.8%, p =0.771), only minor complications were observed in the subjects of the two groups with no need for further treatment. In addition, the duration of simultaneous localization procedures was significantly longer than that of single localization ones (24±7.5 vs 13±6 min, p <0.001). Conclusions: CT-guided simultaneous microcoil localization for multiple pulmonary nodules before VATS was clinical feasible and safe with acceptable increasing the procedure time. Compared with localization for a single pulmonary nodule, simultaneous microcoil localizations for multiple nodules were prone to the occurrence of pneumothorax and hemorrhage. However, no statistically significant differences were observed between the two groups.
Whole-transcriptome analysis of differentially expressed genes in the mutant and normal capitula of Chrysanthemum morifolium
Authors: Liu Hua , Luo Chang , Chen Dongliang , Wang Yaqin , Guo Shuang , Chen Xiaoxi , Bai Jingyi , Li Mingyuan , Huang Xinlei , Cheng Xi , Huang Conglin
Published: December 2020

Background: Chrysanthemum morifolium is one of the most economically important and popular floricultural crops in the family Asteraceae. Chrysanthemum flowers vary considerably in terms of colors and shapes. However, the molecular mechanism controlling the development of chrysanthemum floral colors and shapes remains an enigma. We analyzed a cut-flower chrysanthemum variety that produces normal capitula composed of ray florets with normally developed pistils and purple corollas and mutant capitula comprising ray florets with green corollas and vegetative buds instead of pistils.Results: We conducted a whole-transcriptome analysis of the differentially expressed genes (DEGs) in the mutant and normal capitula using third-generation and second-generation sequencing techniques. We identified the DEGs between the mutant and normal capitula to reveal important regulators underlying the differential development. Many transcription factors and genes related to the photoperiod and GA pathways, floral organ identity, and the anthocyanin biosynthesis pathway were differentially expressed between the normal and mutant capitula. A qualitative analysis of the pigments in the florets of normal and mutant capitula indicated anthocyanins were synthesized and accumulated in the florets of normal capitula, but not in the florets of mutant capitula. These results provide clues regarding the molecular basis of the replacement of Chrysanthemum morifolium ray florets with normally developed pistils and purple corollas with mutant ray florets with green corollas and vegetative buds. Additionally, the study findings will help to elucidate the molecular mechanisms underlying floral organ development and contribute to the development of techniques for studying the regulation of flower shape and color, which may enhance chrysanthemum breeding.Conclusions: The whole-transcriptome analysis of DEGs in mutant and normal C. morifolium capitula described herein indicates the anthocyanin deficiency of the mutant capitula may be related to the mutation that replaces ray floret pistils with vegetative buds. Moreover, pistils may be required for the anthocyanin biosynthesis in the corollas of chrysanthemum ray florets.
Dental students’ perceptions of undergraduate Oral and Maxillofacial Surgery teaching in an integrated curriculum at Kuwait University
Authors: Kamal Mohammad , Abdulwahab Mohammad
Published: December 2020

Background: To evaluate dental students’ perceptions of the undergraduate oral and maxillofacial surgical training teaching curriculum according to a validated questionnaire originally developed by the Association of British Academic Oral Maxillofacial Surgeons (ABAOMS) to assess their self-confidence. Methods: A cross-sectional survey of all 6th-year (n=22) and 7th-year (n=21) dentistry students that was conducted Kuwait University Faculty of Dentistry between 1 and 15 May 2020. A previously implemented and validated questionnaire by ABAOMS was used in the study, which is made of 17 questions assessing various areas of the undergraduate OMFS curriculum in the clinical years. The response options to the questionnaire questions were on a Likert scale. Independent sample t-tests were performed to assess the difference in responses between the two groups. Spearman’s Rho correlations were calculated to measure the strength of association between confidence in all aspects of surgical and forceps exodontia. Statistical significance was considered to be a p-value <0.05. Results: A total of 39 questionnaires were completed by the students. The majority of students expressed that they feel confident that they have enough knowledge to undertake independent practice (61%). General aspects of the questionnaire were answered favourably except for the step of surgical extraction of teeth, in which both classes reported a lower level of confidence. Conclusions: This survey revealed the students’ self-confidence undertaking independent practice and preforming basic oral surgery procedures. Students felt comfortable with exodontia using forceps and elevators, root removal, managing acute pericoronitis, managing haemorrhage from a socket, assessing impacted teeth, and recognising the clinical features of potentially malignant and malignant lesions of the oral cavity. They reported a lower level of confidence for performing the steps of surgical procedures. The ABAOMS survey instrument is a useful and thorough tool to assess the self-confidence of dental students according to their undergraduate oral and maxillofacial surgery teaching.
HSP22 (HSPB8) positively regulates PGF2α-induced synthesis of interleukin-6 and vascular endothelial growth factor in osteoblasts
Authors: Kuroyanagi Gen , Sakai Go , Otsuka Takanobu , Yamamoto Naohiro , Fujita Kazuhiko , Kawabata Tetsu , Matsushima-Nishiwaki Rie , Kozawa Osamu , Tokuda Haruhiko
Published: December 2020

Background: Heat shock protein 22 (HSP22) belongs to class I of the small HSP family that displays ubiquitously expression including in osteoblasts. We previously demonstrated that prostaglandin F2α (PGF2α), a potent bone remodeling factor, induces the synthesis of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) via p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether HSP22 is implicated in the PGF2α-induced synthesis of IL-6 and VEGF, and the mechanism in MC3T3-E1 cells. Methods: MC3T3-E1 cells were transfected with HSP22-siRNA. IL-6 and VEGF release was assessed by ELISA. Phosphorylation of p44/p42 MAP kinase and p38 MAP kinase was detected by Western blotting. Results: The PGF2α-induced release of IL-6 in HSP22 knockdown cells was significantly suppressed compared with that in the control cells. HSP22 knockdown also reduced the VEGF release by PGF2α. Phosphorylation of p44/p42 MAP kinase and p38 MAP kinase was attenuated by HSP22 down-regulation. Conclusions: Our results strongly suggest that HSP22 acts as a positive regulator in the PGF2α-induced synthesis of IL-6 and VEGF in osteoblasts.
Pineal gland transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in STH sheep with two FecB genotypes
Authors: Li Chunyan , He Xiaoyun , Zhang Zijun , Ren Chunhuan , Chu Mingxing
Published: December 2020

Background: long noncoding RNA (lncRNA) has been identified as important regulator in hypothalamic-pituitary-ovarian axis associated with sheep prolificacy. However, little is known of their expression pattern and potential roles in the pineal gland of sheep. Herein, RNA-Seq was used to detect transcriptome expression pattern in pineal gland between follicular phase (FP) and luteal phase (LP) in FecBBB (MM) and FecB++ (ww) STH sheep, respectively, and differentially expressed (DE) lncRNAs and mRNAs associated with reproduction were identified.Results: Overall, 135 DE lncRNAs and 1,360 DE mRNAs in pineal gland between MM and ww sheep were screened. Wherein, 39 DE lncRNAs and 764 DE mRNAs were identified (FP vs LP) in MM sheep, 96 DE lncRNAs and 596 DE mRNAs were identified (FP vs LP) in ww sheep. Moreover, GO and KEGG enrichment analysis indicated that the targets of DE lncRNAs and DE mRNAs were annotated to multiple biological processes such as phototransduction, circadian rhythm, melanogenesis, GSH metabolism and steroid biosynthesis, which directly or indirectly participate in hormone activities to affect sheep reproductive performance. Additionally, co-expression of lncRNAs-mRNAs and the network construction were performed based on correlation analysis, DE lncRNAs can modulate target genes involved in related pathways to affect sheep fecundity. Specifically, XLOC_466330, XLOC_532771, XLOC_028449 targeting RRM2B and GSTK1, XLOC_391199 targeting STMN1, XLOC_503926 targeting RAG2, XLOC_187711 targeting DLG4 were included.Conclusion: All of these differential lncRNAs and mRNAs expression profiles in pineal gland provide a novel resource for elucidating regulatory mechanism underlying STH sheep prolificacy.
Combined Macromolecule biomaterials together with fluid shear stress promote the osteogenic differentiation capacity of equine adipose -derived mesenchymal stem cells
Authors: Elashry Mohamed I. , Baulig Nadine , Wagner Alena-Svenja , Klymiuk Michele C. , Kruppke Benjamin , Hanke Thomas , Wenisch Sabine , Arnhold Stefan
Published: December 2020

Background: Combination of stem cells and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. The present study examined the effect of biomaterial scaffolds on equine adipose-derived mesenchymal stem cells (MSCs) morphology, viability, adherence, migration and osteogenic differentiation. Methods: MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and combined B30 with strontium (B30Str) biomaterials in osteogenic differentiation medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, live cell imaging, cell viability, adherence and migration assays, semi-quantification of alkaline phosphatase (ALP) activity and quantification of the osteogenic markers runt related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression. Results: The data revealed that combined mechanical fluid shear stress (FSS) with MC but not B30 enhanced MSCs viability and promoted their migration. Combined osteogenic medium with MC, B30 and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30 and B30Str resulted in diffused matrix mineralization. The combined osteogenic induction with biomaterials under mechanical FSS increased Runx2 protein expression either in comparison to those cells cultivated in basal medium (BM) or those cells induced under static culture. Runx2 and ALP expression was upregulated following combined osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture. Conclusions: Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.
Occult Foreign Body Aspirations in Pediatric Patients: 20-years of Experience
Authors: Liu Bo , Ding Fengxia , An Yong , Li Yonggang , Pan Zhengxia , Wang Gang , Dai Jiangtao , Li Hongbo , Wu Chun
Published: December 2020

Background: The purpose of our study was to assess the frequency of occult foreign body aspiration (FBA) and to evaluate the diagnostic difficulties and therapeutic methods for these patients. Methods: Between May 2000 and May 2020, 3557 patients with the diagnosis of FBA were treated in our department. Thirty-five patients with occult FBA were included in this study. A retrospective analysis of medical records was performed. Results: Twenty-three male patients (65.7%) and 12 female patients (34.3%) were hospitalized due to occult FBA. The average age was 3.60 years (range 9 months-12 years). Most of the patients were younger than 3 years old (n=25, 71.4%). Coughing (n=35, 100%) and wheezing (n=18, 51.4%) were the main symptoms and signs. All the patients were found to have a FBA under the fiberoptic bronchoscope. The most common organic foreign bodies were peanuts (n=10) and the most common inorganic foreign bodies were pen caps (n=5). The extraction of foreign bodies under rigid bronchoscopy was applied successfully in 34 patients. Only one patient needed a surgical intervention. Conclusions: Occult FBA should always be considered in the differential diagnosis of chronic or recurrent respiratory diseases that are poorly explained, even in the absence of a previous history of aspiration.
Interleukin-6 promotes primitive endoderm development in bovine blastocysts
Authors: Wooldridge Lydia K. , Ealy Alan
Published: December 2020

Background: Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two cell types. Results: Increases (P<0.05) in total ICM cell numbers and PE cell numbers were detected in bovine blastocysts at day 8 and 9 post-fertilization after exposure to 100 ng/ml recombinant bovine IL6. Also, IL6 increased (P<0.05) the number of undifferentiated ICM cells (cells containing both PE and EPI markers). The effects of IL6 on EPI cell numbers were inconsistent. Studies were also completed to explore the importance of Janus kinase 2 (JAK2)-dependent signaling in bovine PE cells. Definitive activation of STAT3, a downstream target for JAK2, was observed in PE cells. Also, pharmacological inhibition of JAK2 decreased (P<0.05) PE cell numbers. Conclusions: To conclude, IL6 manipulates ICM development after EPI/PE cell fates are established. The PE cells are the target for IL6, where a JAK-dependent signal is used to regulate PE numbers.
Three-Year Longitudinal Skill-based Teaching Curriculum for All Residents
Authors: Rowat Jane , Johnson Krista , Antes Lisa , White Katherine , Rosenbaum Marcy , Suneja Manish
Published: December 2020

BACKGROUND. Despite significant teaching responsibilities and national accreditation standards, most residents do not receive adequate instruction in teaching methods. Published reports of residents-as-teachers programs vary from brief one-time exposures to curricula delivered over several months. A majority of interventions described are one or two-day workshops with no clear follow-up or reinforcement of skills. A three-year longitudinal teaching skills curriculum was implemented with these goals: 1) deliver an experiential skill-based teaching curriculum allowing all residents to acquire, practice and implement specific skills; 2) provide spaced skills instruction promoting deliberate practice/reflection; and 3) help residents gain confidence in their teaching skills. METHODS. One hundred percent of internal medicine residents (82/82) participated in the curriculum. Every 10 weeks residents attended a topic-specific experiential skills-based workshop. Each workshop followed the same pedagogy starting with debriefing/reflection on residents’ deliberate practice of the previously taught skill and introduction of a new skill followed by skill practice with feedback. Every year, participants completed: 1) assessment of overall confidence in each skill and 2) retrospective pre-post self-assessment. A post-curriculum survey was completed at the end of three years. RESULTS. Residents reported improved confidence and self-assessed competence in their teaching skills after the first year of the curriculum which was sustained through the three-year curriculum. The curriculum was well received and valued by residents. CONCLUSIONS. A formal longitudinal, experiential skills-based teaching skills curriculum is feasible and can be delivered to all residents. For meaningful skill acquisition to occur, recurrent continuous skill-based practice with feedback and reflection is important.
The Mediating Effect of Overcompensation of Rationalisation on Instrumental Climate and Escalation of Corruption Relationship
Authors: Nahartyo Ertambang , Rohma Frida Fanani , Mursita Lufi Yuwana
Published: December 2020

This paper employs cognitive dissonance theory to investigate the mediating effect of Overcompensation of Rationalisation (OCR) on instrumental climate and escalation of corruption relationship. Moreover, this study examines the role of loyalty as a moderator to reduce the impact of instrumental climate on OCR. This study uses laboratory experiments with 94 master’s degree students as participants. The results show that OCR fully mediates the relationship between the instrumental climate and the escalation of corruption. Instrumental climate leads an individual to OCR, which impacts on the corruption escalation. The analysis also shows that loyalty can moderate the effect of instrumental climate on OCR. It indicates that a level of loyalty enables an individual to filter the impact of instrumental climate on OCR. Besides, the intention of corruption also contributes to the escalation and snowballing effect of corruption. Surprisingly, it is also found that individuals with their experiences tend to be more expert, skilful, and efficient in committing acts of corruption. Those enable the perpetrator to be corrupt smoothly; the amount of corruption, in aggregate, is more massive without the increase of maximum scores so it can lead the evaluator on.
Impact of Internal CSR Perception on Affective Organisational Commitment among Bank Employees
Authors: Tien Dung Luu
Published: December 2020

The present study aims to investigate the relationship between perceived internal corporate social responsibility (CSR) practices and affective organisational commitment. Based on a primary data sample of 300 bank employees, a structural equation model (SEM) was developed to test the research hypotheses. The results indicated that the research model fitted well with the empirical data. The findings show that employee perception of internal CSR dimensions was significantly related to affective organisational commitment. Therelationship between affective commitment and internal CSR was also investigated within a model that moderates effects on employee gender. This study provides an understanding that motivations could encourage employee’s affective commitment, which might translate to effective human resource management policies.
Investigating the Factors of Consumers’ Purchase Intention Towards Life Insurance in Bangladesh: An Application of the Theory of Reasoned Action
Authors: Nomi Marzia , Sabbir Md. Mahiuddin
Published: December 2020

This study aims to examine the factors that influence consumer purchase intention towards life insurance. The study included religiosity, risk aversion motives, saving motives, and financial literacy with classical Theory of Reasoned Action (TRA), therefore extending the model. A convenience sampling method was employed to collect data from 315 respondents working in different public and private institutions in Bangladesh. Collected data were analysed using the structural equation model (SEM). The results revealed that attitude, subjective norms, risk aversion motives, saving motives, and financial literacy have a significant positive impact on consumer purchase intention towards life insurance. Moreover, religiosity was found to have a negative impact on purchase intention. In addition, saving motives was found as a mediator in the relationship between risk aversion motives and purchase intention, as well as between financial literacy and purchase intention. Since attitude and subjective norms were found to have the most effect on purchase intention, the study implicated that marketers should emphasise company image, reputation, and the credibility of the agents, together with the significant others to whom we often turn to before making any financial decision. Finally, the study offered directions for further research after divulging several limitations.
The Influence of Social Media Marketing on Apparel Brands’ Customers’ Satisfaction: The Mediation of Perceived Value
Authors: Yang Yongzhong , Khan Zobi , Zhang Yu
Published: December 2020

The development of social media has changed traditional media by providing a platform to conduct marketing and advertising activities. Nowadays, social media offers the opportunity to build a relationship between customers and brands. The strategic use of social media marketing has significant importance for customers as well as for marketers. In this paper, the influence of social media marketing activities (SMMAs) is assessed on apparel brands’ perceived value and Chinese customers’ satisfaction. The data was collected through a quantitative survey-based approach from Chinese social media and apparel brands’ users (n = 355). The collected data from respondents were analysed through the confirmatory factor analysis (CFA) and Hayes PROCESS macro. Based on our findings, it is noted that SMMAs play an essential role in improving the brand perceived value and customers’ satisfaction in China. Moreover, it is also concluded that the apparel brand’s perceived value significantly mediates the relationship between SMMAs and customers’ satisfaction. This study provides several theoretical and practical guidelines to the marketers in China to effectively implement their advertising activities on social media platforms.
Callus Induction and Micropropagation of Lilium candidum L. Using Stem Bulbils and Confirmation of Genetic Stability via SSR-PCR
Authors: TOKGÖZ Hilal Büşra , ALTAN Filiz
Published: December 2020

Natural populations of Lilium candidum L. are remarkably affected by biotic and abiotic factors therefore there is a requirement to develop effective micropropagation protocol to provide mass production, multiplication and conservation of these plants. For this reason, this study was aimed to develop an efficient micropropagation method for multiple shoot production via somatic embryogenesis induced from L. candidum stem bulbils and also to determine the genetic stability of in vitro grown plants using SSR markers. The obtained results of this study are the first comprehensive reports including an investigation of genetic fidelity on somatic embryogenesis of L. candidum. After surface sterilization of bulbils, the calculated regeneration percentage of them was 89.5% and the callus induction was achieved using leaf segments of in vitro grown bulbils. The well formed somatic embryos were obtained from smooth whitish-yellow colored calli and these somatic embryos produced well formed healthy L. candidum seedlings with adventitious roots. All rooted seedlings were easily adapted to greenhouse conditions and the genetic stability of in vitro grown seedlings were determined by using SSR-PCR technique and it was calculated as 100%.
ŞİDDET İÇERİKLİ SÖYLEMLER BAKIMINDAN İFADE ÖZGÜRLÜĞÜNÜN SINIRLANDIRILMASI: AİHM KARARLARI IŞIĞINDA KISA BİR DEĞERLENDİRME
Authors: ÖNGÜÇ Bahar
Published: December 2020

Her türlü kanaati serbestçe açıklama hürriyeti anlamına gelen ve insanın insan olmasından kaynaklı onur değere sahip olan ifade özgürlüğü, birçok temel hak ve hürriyetin teminatı olma özelliğini taşımaktadır. Bu bağlamda ifade özgürlüğünün tam olarak korunmadığı toplumlarda diğer hak ve hürriyetlerden bahsetmek çok da mümkün değildir. Bunun yanısıra, ifade özgürlüğünün sınırsız bir hak olmadığı, tam aksine demokratik toplumlarda bir takım kısıtlamalara tabi olduğu kabul görmektedir. Özgürlüğe ilişkin temel prensip getiren AİHS’nin 10. maddesi aynı zamanda bu özgürlüğün bir takım sınırları olduğunu vurgulamaktadır. Sözleşme’nin 10/2. maddesinde tahdidi bir biçimde sayılan meşru amaçların varlığı halinde bu özgürlüğün sınırlanabileceği öngörülmüştür. Bu amaçlardan herhangi birinin yokluğu halinde ise AİHM tarafından ifade özgürlüğünün ihlal edildiği kabul edilmektedir. Bu çalışma, AİHS'nin 10.maddesi çerçevesinde şiddet içerikli söylemlerin konu edildiği ve Türkiye’nin taraf olduğu bazı AİHM kararlarını ele almaktadır. AİHM, şiddet içerikli söylemlerin şiddete çağrı yapıp yapmadığını araştırırken her somut durumun kendine özgü hallerini de göz önünde bulundurarak konuya ilişkin farklı bir yaklaşım benimsemekte ve kaynak niteliği taşıyan içtihatlar geliştirmektedir.
Pulmonary vein isolation using cryoballoon technique in atrial fibrillation patient after Greenfield vena cava filter implantation
Authors: Chen Shaojie , Schmidt Boris , Bordignon Stefano , Tohoku Shota , Chun K R Julian
Published: December 2020

Background: Cryoballoon ablation is an established procedure for atrial fibrillation (AF). Patient with vena cava filter undergoing pulmonary vein isolation (PVI) were seldom reported.Case presentation: We describe an AF ablation technique using the second generation cryoballoon in a patient after vena cava filter implantation. All pulmonary veins were successfully isolated without complication.Conclusions: For AF patient with previously implanted vena cava filter, cryoballoon based PVI appears feasible and safe.
Microstructure of the juvenile sheep aortic valve hinge region and the trilamellar sliding hypothesis
Authors: Yacoub Magdi , Tseng Yuan-Tsan , Mitchelson Brian , Latif Najma
Published: December 2020

Background: The aortic valve mechanism performs extremely sophisticated functions which depend on the microstructure of its component parts. The hinge mechanism of the aortic leaflets plays a crucial part in the overall function. However, the detailed microstructure and its relation to function has not been adequately studied.Methods: The aortic roots of juvenile sheep were fixed under physiologic pressure. Sections through all three sinuses were then performed to illustrate the microstructure of the hinge mechanism in different regions of the aortic root.Results: The hinge region in the different sinuses showed unique microstructure with a trilamellar topology with a dominant core consisting of glycosaminoglycans. The exact arrangement of the trilamellar structures varies around the aortic sinuses, which could have functional implications. These features allow the hinge to perform its complex functions through what we have described as “the trilamellar sliding hypothesis”.Conclusion: The microstructure of the hinge mechanism is unique and enables it to perform it sophisticated functions.
Prevalence of cardiovascular events in genetically confirmed versus unconfirmed familial hypercholesterolaemia
Authors: Khan Tina Z , Breen Jane , Neves Emma , Grocott-Mason Richard , Barbir Mahmoud
Published: December 2020

Introduction: Genetic testing for familial hypercholesterolaemia (FH) is not yet established for widespread use internationally to provide diagnostic confirmation, in part due to high cost and resource requirement. We need to establish whether genetic testing is clinically justified in terms of risk stratification and prediction of cardiovascular events.Methods: We performed a single tertiary cardiac centre retrospective evaluation of patients with FH managed within our genetic screening service. We evaluated the prevalence of cardiovascular events in genetically confirmed cases of FH compared to those unconfirmed upon genetic testing, to assess whether gene positivity confers a higher risk phenotype. We also compared the clinical characteristics of the genetically confirmed and unconfirmed group.Results: Amongst adult patients (≥18 years) with genetically confirmed heterozygous FH (n=87), 34% (30/87) had one or more documented CV events. In comparison a lower event rate was observed in adult patients with genetically unconfirmed FH (n=170) with 25% (42/170) experiencing one or more documented CV events. Additional cardiovascular risk factors were more prevalent in the unconfirmed group including hypertension, co- morbidities, higher age and body mass index which may have modified the difference in cardiovascular risk.Conclusion: Genetic testing in FH may be clinically justified and appears to identify a subset of patients with higher risk of cardiovascular events. However, the risk difference is modified by alternative cardiovascular risk factors and co-morbidities which may be more prevalent in genetically unconfirmed FH.
Raising awareness for rheumatic mitral valve disease
Authors: Passos Livia SA , Nunes Maria Carmo P , Zilla Peter , Yacoub Magdi , Aikawa Elena
Published: December 2020

Rheumatic heart disease (RHD) is a major burden in low- to mid-income countries, where each year it accounts for over a million premature deaths associated with severe valve disease. Life-saving valve replacement procedures are not available to the majority of affected RHD patients, contributing to an increased risk of death in young adults and creating a devastating impact. In December 2017, a group of representatives of major cardiothoracic societies and industry, discussed the plight of the millions of patients who suffer from RHD. A comprehensive solution based on this global partnership was outlined in "The Cape Town Declaration on Access to Cardiac Surgery in the Developing World". The key challenge in controlling RHD is related to identification and removal of barriers to the translation of existing knowledge into policy, programs, and practice to provide high-quality care for patients with RHD. This review provides an overview on RHD by emphasizing the disease medical and economic burdens worldwide, risk factors, recent advance for early disease detection, and overall preventive strategies.
The utility of 3D printed models in complex percutaneous paravalvular leak interventions
Authors: ElGuindy Ahmed , Osman Ahmed , Elborae Ahmed , Nagy Mohamed
Published: December 2020

Paravalvular leaks (PVL) are seen in 5-17% of patients after surgical mitral and aortic valve replacement. This is usually well-tolerated in the majority of patients; however, up to 5% will require re-intervention due to either hemodynamically significant regurgitation or hemolysis requiring repeated blood transfusion. Transcatheter closure of PVLs is becoming the treatment of choice in many patients owing to the high risk of redo surgery, high rates of recurrence with the surgical approach, and substantial improvements in device technology and growing expertise in structural heart disease interventions. Careful selection of the appropriate candidates by the Heart Team with in-depth analysis of clinical and multimodality imaging data is critical to ensuring good short- and long-term outcomes.The defect is usually oval/ crescentic and often serpiginous in nature, which poses significant challenges in the optimal size and number of devices to implant - especially with large size defects. Generally, defects involving more than 25-30% of the sewing ring are generally deemed unsuitable for percutaneous closure. While the Amplatzer family of vascular plugs (e.g. AVP3 and AVP2) is commonly used for percutaneous closure of PVLs, there are currently no approved dedicated devices for this indication, except the paravalvular leak device (Occlutech) which is not universally available. Small and relatively circular defects can usually be closed using a single plug, conventionally utilizing a size that is 25-30% larger than the mean diameter of the defect. Larger and crescentic defects on the other hand frequently require more than one plug and can be quite challenging in terms of choosing the appropriate size(s).We report two cases with very large defects with irregular shape in which 3D printed modeling was extremely useful for bench testing to optimize the number and sizes of devices to be implanted.
Microvascular dysfunction in septic and dengue shock: Pathophysiology and implications for clinical management
Authors: McBride Angela , Chanh Ho Q , Fraser James F , Yacoub Sophie , Obonyo Nchafatso G
Published: December 2020

[No abstract, showing first paragraph of article]The microcirculation is the terminal vascular network of the systemic circulation, whose primary function is to distribute oxygen to, and remove metabolic by-products from living cells. In health, tissue perfusion is regulated by control of microvascular tone and endothelial permeability. In states of shock, however, there is a mismatch between demand for, and delivery or utilisation of oxygen in the tissues.
PRELIMINARY ANALYSES OF THE EFFICIENCY OF SUSTAINABLE FILTERS AND HYDROELETRIC POTENTIAL FOR A CLEAN ENERGY GENERATION AND WATER DEPOLLUTION SYSTEM
Authors: De Souza Junyor Flavio , Da Silva Creir , Andrich Filipe , Ronquim Joyce , Pereira Máriam Trierveiler
Published: December 2020

In last decades, water pollution has become more pronounced on all rivers in Latin America, Africa and Asia. Thus, it is estimated that the deterioration of water quality will extend further over the coming decades, increasing threats to human health, environment and sustainable development. Simultaneously, it has been observed a depletion of non-renewable energetic sources and its increasing replacement by renewable sources. Therefore, the United Nations (UN) has been setting goals regarding sustainable development for the countries. Thus, the present study aimed to create and design a mechanism that integrates clean energy generation and water depollution from small and medium-sized streams by using agro-industrial waste. For this, it was necessary to survey the theoretical framework besides the hydrological study of Pinhalzinho II watershed, which was chosen for the simulation. Thus, it was started the design of the hydroelectric microcentral coupled to a sustainable water purification system using the softwares: AutoCAD© and SketchUp 3D©. In addition, the stream flow was determined and its energy potential was calculated in order to estimate the amount of energy that could be generated by the system. Then, laboratory scale filters were constructed from sugarcane bagasse and cassava peel for efficiency analyses. Finally, the upstream (raw water) and downstream (after filtering) water samples were analyzed and their parameter values determined.  After data treatment, it was found that the results were not satisfactory; conversely, they showed a reduction in raw water quality, achieving efficiency only for the thermotolerant coliforms parameter. Nevertheless, regarding the hydroelectric microcentral project and the hydroenergetic potential of the stream, the results were promising.
GENERATION OF RENEWABLE ENERGY THROUGH ROADS, BRIDGES AND CYCLE PATHS
Authors: Gorges Gustavo de Carvalho , Wedekind Johanes Paulo , Kimura Gabriela Akemi Perez , Rosella Jéssica Wasny Martins , Herrero Welysson , Wedekind Joyce Ronquim
Published: December 2020

Abstract: The economic growing and technological development brought advantages to society. However, the adoption of the sustainable development is necessary so the future generations will not be compromised. In this way, the present work has as its objective presenting a bibliographical review about roads, bridges and bike lanes that generate renewable energy. The photovoltaic panels are very important to generate energy on the roads and, as an example, the acoustic barriers on the roads can be exchanged or implemented with photovoltaic panels to maintain the function of sound barrier and of generator of electric power at the same time. Another way of generating renewable energy are bike lanes, in the Netherlands, as an example, it is utilized a special concrete, superimposed by a layer of highly resistant glass with photovoltaic cells, so the installed floor can convert sunlight into energy. The piezoelectric energy can also generate energy in bike lanes using a pressure-sensitive floor with friction from bicycle tires. When it comes to bridges, a project developed in Italy intends to place solar panels and wind generation turbines in a bridge as a way of generating energy, generating around 40 million kW/h in one year. The proposal of this bridge is to fulfill empty spaces among the existing pillars on the bridge to integrate the system of wind turbines in the structure. It can be concluded that, throughout time, the renewable energies have been standing out and civil construction has shown an important role in this sector, presenting technological solutions and taking advantage of spaces in relation to the implantation of roads, bridges and bike lanes.Key words: Sustainability. Renewable Energy. Photovoltaic Plates. Piezoeletric Energy. Wind Energy.
ART PIXEL PANELS: ENVIRONMENTAL EDUCATION AND ART IN TEACHER TRAINING
Authors: De Oliveira Adeilson Moizés , De Lima Ivã Vinagre , Barbado Norma
Published: December 2020

Solid waste improperly disposed causes several environmental problems and affects public health. Especially plastic, produced on a large scale and incorrectly discarded, can negatively affect terrestrial and aquatic ecosystems. In this sense, this study aimed to sensitize the academic community of the Federal Institute of Paraná Campus Umuarama in relation to the inadequate disposal of waste through the reuse of plastic lids in artistic productions, activities here called "art pixel panels". To this end, undergraduate students in Biological Sciences promoted awareness and collection of plastic caps in potential generators of this waste. At the institution, based on figures researched on the internet and others of their own creation, the panels were constituted with tying schemes for these covers. To carry out the tying, internal wiring extracted from network cables was used, which were already in disuse, in view of their resistance and convenience in handling. Thus, 20 panels were made using more than 30,000 plastic caps. The art pixel panels were displayed in the lobby causing the academic community to reflect on the generation and final destination of plastic, in addition to serving as a source of artistic inspiration. It is believed that, through environmental education and art, it is possible to promote the formation of more aware and responsible people regarding current environmental problems.
PROPOSAL FOR THE DEVELOPMENT OF AN URBAN SUSTAINABILITY INDICATOR FOR THE MUNICIPALITY OF UMUARAMA/PR
Authors: Soares Solano Ribeiro , Soares Samuel Ronobo , Pereira Máriam Trierveiler
Published: December 2020

The current model for society’s development, based on the unconscious use of natural resources, points to signs of exhaustion, requiring a reflection in order to formulate strategies that overcome the challenge of coexistence between the relationship between man and nature. The use of tools such as sustainable indicators has been shown to be effective in the identification and understanding of socio-environmental phenomena and in the subsequent decision making by public management, assisting in the resolution of problems, and consequently, acting as a resource for sustainable development. This study aims to propose the development of an urban sustainability indicator for the municipality of Umuarama, in the state of Paraná, Brazil. In order to build the indicator, data from different aspects linked to Sach’s sustainability model are to be collected, and later put into statistical treatment to standardize it and make its comparison possible, resulting in its index, which is expected to analyze the current state of the local sustainability and sustainable development.
SOIL PROTECTION BY USED OF COVER CROPS CONSORTIA AND SINGLE CULTIVATION
Authors: Jungbluth Daiana , Dahlem Ziech Ana Regina , Pereira Camila Roberta , Dos Santos Márcia Cristina , Machado Patrick
Published: December 2020

The no-till system has been growing over the years and for this system to be successful, it is essential to maintain permanent vegetation cover over the soil, an adequate crop rotation system with minimal overturning. A strategy for soil protection is to introduce species of cover crops in winter under single or intercropping. The objective was to evaluate the rate of soil cover by intercropping between black oats (Avena strigosa L.) and forage turnip (Raphanus sativus L.) at different sowing densities, as well as the isolated species in terms of soil protection under no-tillage. The study was conducted at the Federal Technological University of Paraná (UTFPR), campus Santa Helena, with a randomized block design, with five treatments and three repetitions. The treatments were: 100% black oats (BO); 100% forage turnip (FT); 75% BO + 25% FT; 50% BO + 50% FT and 25% BO + 75% FT. The cover crops were sown in May 2019. The percentage of soil cover from 21 to 91 days after sowing (DAS) was evaluated using the photographic method, with weekly collection of images in an area delimited by a metallic frame (25 m²), positioned on the ground at two fixed points per plot. The coverage rate quantification was estimated by overlaying a grid with 100 points of intersection over each image. The rate of soil cover by consortia and single crops did not show a statistically significant difference. To 49 days, consortia had coverage equal to or greater than 70%, while for single species, this percentage was reached at 56 DAS and 70 DAS, for BO and FT, respectively. All treatments showed high potential for soil protection and coverage rate from 70 DAS.
CHEMICAL AND THERMOANALYTICAL CHARACTERIZATION OF THE PINK PEPPER (Schinus terebinthifolius Raddi) SEEDS ESSENTIAL OIL
Authors: Picolotto Angela Maria , Ariati Angela Maria , Franciscato Lidaiane Mariáh Silva dos Santos , Bittencourt Paulo Rodrigo Stival , Caramão Elina Bastos , Sakai Otávio Akira , Moritz Cristiane Mengue Feniman
Published: December 2020

The essential oil (EO) of pink pepper has important biological properties that can enhance its application in the pharmaceutical, cosmetic and food industries. The objective of this study was to characterize the EO of pink pepper seeds in terms of their chemical composition and thermophysical properties. Certified pink pepper seeds obtained commercially were used. The EO was extracted by hydrodistillation and chemically characterized in a gas chromatograph coupled to mass spectrometer (GC/MS). The thermoanalytical characterization was performed on a simultaneous high resolution equipment, and the Thermogravimetric Analysis (TG) and Differential Scanning Calorimetry (DSC) were used to determine the physical-chemical and kinetic properties of the OE in the room temperature range up to 250 ºC in the 20 ºC.min-1 ratio under nitrogen atmosphere. The Arrhenius kinetic model was applied to determine the activation energy of the EO. A total of 51 chemical compounds were detected in the pink pepper EO, of which 42 compounds were identified (54.7% of monoterpene compounds and 45.3% sesquiterpenes). The major components were ?-pinene (26.80%), germacrene D (11.25%), sabinene (11.13%) and terpinen-4-ol (8.59%). The thermogravimetric analysis indicated a single stage of thermo-degradation of the pink pepper EO between 74.8 ºC and 184.7 ºC, being an endothermic peak in the physical transition of vaporization. The variation of enthalpy and activation energy of EO were found with values of  = - (39.8 ± 9.5) KJ.Kg-1 and  = - (2,546 ± 0.033) KJ.mol-1, respectively. The data presented in the thermogravimetric analysis of pink pepper seeds EO allowed observing important thermal parameters for the definition of industrial processes that can use it as raw material, so that its biological activities can remain stable under controlled temperature conditions and below the range degradation.