bluuebunny/crossref_metadata_2025 huggingface dataset:
Dietary Effects of Probiotic Bacteria, Bacillus amyloliquefaciens AV5 on Growth, Serum and Mucus Immune Response, Metabolomics, and Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
Authors: Shija Vicent Michael , Zakaria Glory Emanuel , Amoah Kwaku , Yi Li , Huang Junwei , Masanja Fortunatus , Yong Zhong , Cai Jia
Published: August 2024

In the present study, we investigated the effect of dietary supplementation with the probiotic Bacillus amyloliquefaciens AV5 (OR647358) on the growth, serum and mucus immune responses, metabolomics, and lipid metabolism of Oreochromis niloticus. Fishes (27.2 ± 1.7 g and 9.0 ± 1.2 cm) were fed three distinct meals: a commercial diet (control‐GC) and two treatment diets supplemented with probiotics at 106 (G1) and 108 cfu/g (G2), respectively, for 30 days. In the G2 group, the final weight, specific growth rate, weight gain rate, survival rate, and feed conversion ratio of the fish were significantly improved (p < 0.05). Lysozyme, myeloperoxidase, and alkaline phosphatase activities in the mucus of fish were significantly higher (p < 0.05) in the G1 and G2 groups. The serum total protein, superoxide dismutase, glutathione peroxidase, reactive oxygen species, and reactive nitrogen species levels were noticeably higher (p < 0.05) in fish fed G1 and G2. In addition, in the G1 and G2 groups, higher levels of enzymes involved in lipid metabolism, such as pyruvate kinase, 2‐hydroxyethyl‐ThPP, and dihydrolipoamide dehydrogenase, were increased. Distal gastrointestinal metabolites, such as glycerophospholipids and histidine, were observed. These findings strongly indicate that incorporating B. amyloliquefaciens AV5 at 108 cfu/g into commercial feeds positively influences fish growth, immunity, and lipid metabolism.
Two Unrelated Iranian Patients with Adenosine Deaminase 2 Deficiency: A Case Report and Review of Treatment
Authors: Karimzade Parvaneh , Eghbali Aziz , Keramatipour Mohammad , Shiari Reza , Golchehre Zahra , Taghizadeh Mahdieh , Fallahi Mazdak , Fallah Shahrzad , Khakbazan Fard Nasrin , Eslami Narges , Bazgir Narges , Jamee Mahnaz , Chavoshzadeh Zahra
Published: August 2024

Background. Adenosine deaminase deficiency 2 (DADA2) is an autoinflammatory disorder, caused by the CECR1 gene mutation. The major clinical manifestations include recurrent vasculitis, neurological disorders such as stroke, hematologic abnormalities, and immunodeficiency. As reported in previous studies, DADA2 may be manifested by ischemic or hemorrhagic strokes. This disorder also includes various hematological manifestations (pure red cell aplasia, pancytopenia, hemolytic anemia, and pancytopenia with bone marrow involvement). Case Presentation. In this case report, we present the clinical and immunological findings of two unrelated patients with DADA2. The first patient was a 7‐year‐old female who experienced recurrent neurological symptoms such as vertigo, tinnitus, hearing loss, and right‐sided hemiparesis. Her brain magnetic resonance imaging (MRI) revealed a left‐sided stroke, and she responded well to antitumor necrosis factor alpha agents and plasmapheresis. The second patient was a 6‐year‐old female who had recurrent fever and bicytopenia, aphthous lesions, cervical lymphadenopathy, and elevated liver enzymes. We also discussed the strategies used to manage the clinical manifestations in these two DADA2 patients. Conclusion. In this case report, we discussed two cases with DADA2 deficiency and their respective manifestations. The first case showed neurological symptoms while the second case had hematological symptoms. Although there is no established treatment for DADA2 due to its rarity, steroids are commonly used to treat this disorder. Antitumor necrosis factor is also effective in controlling the symptoms, especially the neurological ones. In cases where there is no appropriate response to these treatments, hematopoietic stem cell transplantation can be beneficial.
Factors Influencing Consumers’ Acceptance of Smartphone Diet Applications: An Integrated Model
Authors: Mohammed Abdulalem
Published: August 2024

The rapid expansion of smartphone‐based diet applications has been driven by the growing prevalence of mobile technology among consumers and their diverse lifestyle preferences. This study seeks to analyze the factors influencing the use of these applications. The conceptual framework combines three models: the unified theory of acceptance and use of technology (UTAUT2), the DeLone and McLean information system (IS) (D&M IS) success model, and the protection motivation model. Conducted in Saudi Arabia, the research employed a quantitative methodology with a survey questionnaire administered to 572 customers. The study, validated through partial least squares (PLS) structural equation modeling (PLS‐SEM), identified several significant predictors for the intention to use smartphone diet applications. These include performance expectancy (PE), effort expectancy (EE), social influence (SI), price value (PV), IS, and service quality (SEQ), as well as perceived vulnerability and perceived severity. Contrary to expectations, factors like facilitating conditions (FCs) and hedonic motivation (HM) did not significantly impact behavioral intention. This pioneering study sheds light on the adoption of smartphone diet applications in an emerging economy, offering valuable insights for scholars, developers, and health professionals.
Genetic and Pathogenic Characteristics of an Emerging Highly Virulent Recombinant Lineage Korean Clade C PRRSV Strain
Authors: Suh Jeongmin , Chae Chanhee
Published: August 2024

A strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated from lung tissue of a pig showing severe respiratory clinical signs from a farm in Gyeongsang province of South Korea. This PRRSV strain, designated as SNUVR220803, was classified within the lineage Korean clade C (LKC) based on a phylogenetic analysis of the ORF5 gene. A whole‐genome analysis was conducted on the SNUVR220803 strain, which appears to be a recombinant between the PRRSV strains K07‐2273 (part of LKC lineage) and Ingelvac MLV (part of Lineage 5). The Nsp2 amino acid sequence of this strain features a deletion of four additional amino acids, setting it apart from the typical Korean clades A, B, and C lineages. An animal inoculation experiment was conducted with 24 pigs divided into three groups: 12 pigs in the inoculated group, six in the sentinel group, and six in the negative control group. Inoculated pigs exhibited persisting hyperthermia (≥40.3°C) for 5 days, palpebral edema, and cyanosis. Subsequently, these pigs suffered from severe respiratory distress and cachexia, leading to a mortality rate of 58.3% (7 out of 12 pigs) at 14 days postinoculation (dpi). Body weight decreased post‐SNUVR220803 strain infection in both the inoculated and sentinel groups. Gross pathology revealed noncollapsed lungs and serous effusion in the pericardial and peritoneal cavities. Microscopic analysis revealed severe interstitial pneumonia, while immunohistochemistry confirmed the presence of PRRSV antigen in the lungs, lymph nodes, thymus, kidneys, and the heart. Additionally, the levels of cytokines such as tumor necrosis factor‐α (TNF‐α), interferon‐α (IFN‐α), and IL‐10 were significantly elevated in the plasma of infected pigs. These observations indicate that the LKC recombinant strain, combined with Lineage 5, possesses high virulence and infectivity as characterized by distinctive exudative lesions.
Rod and Crossbone P3HT Aggregates in P3HT‐Perylene‐Diimide Solar Cells
Authors: Vasant Rushil , Yoo Junghwa , Bisconti Benjamin , McNelis Brian J. , Barber Richard P.
Published: August 2024

We present efficiency and lifetime results for solar cells composed of blends of poly(3‐hexylthiophene‐2,5‐diyl) and N,N′‐bis(3‐pentyl)perylene‐3,4,9,10‐bis(dicarboximide) or P3HT:PDI. This combination represents a baseline system for future studies of PDIs as electron acceptors. Poor performance in the fabricated devices was correlated with unusual sample morphologies. Large aggregates in the form of rods and crossbones were observed using both optical and electron microscopy. Energy dispersive X‐ray elemental analysis was used to identify the composition of the structures. These results illustrate the critical connection between polymer organization and solar cell efficiency, providing insights into the structure‐function interaction between PDI and P3HT.
Impact of Hepatic Portal Venous Gas on the Prognosis of Traumatic Out‐of‐Hospital Cardiac Arrest: A Reason to Consider Terminating Cardiopulmonary Resuscitation
Authors: Yeom Seok Ran , Min Mun Ki , Lee Dae Sup , Lee Min Jee , Chun Mo Se , Park Sung Wook , Yang Wook Tae
Published: August 2024

Background. We evaluated the prognosis of traumatic out‐of‐hospital cardiac arrest (OHCA) by assessing the presence of hepatic portal vein gas (HPVG) observed in ultrasound (US) or point‐of‐care ultrasonography (POCUS) performed during CPR. Furthermore, we aimed to understand the role of HPVG in decision‐making regarding CPR discontinuation or withholding in traumatic OHCA. Methods. The retrospective study was conducted at the level 1 trauma center of urban academic medical centers in South Korea. We included adult trauma OHCA patients who underwent CPR between January 1, 2020, and June 30, 2022. Data on traumatic OHCA patients who presented to the level I trauma center during this period were extracted from the hospital’s electronic medical record system. The arrest data were separately managed through the hospital’s electronic medical record system for quality control, specifically the arrest registry. US images or clips of the hepatic portal vasculature (HPV) during CPR were used to assess the presence of HPVG. These images were independently reviewed by two emergency medicine physicians with several years of US examination experience who were blinded to all clinical details and outcomes. We evaluated the prognosis of traumatic OHCA by assessing the presence of HPVG using the US. In addition, we analyzed the general characteristics and assessed the impact on the ROSC in traumatic OHCA. Results. Among the 383 cardiac arrest patients, 318 traumatic OHCA patients were included. The mean age was 54.9 ± 19.4 years, and most patients were male. The initial rhythm was mainly asystole, and falls were the most frequent cause of injury. The overall ROSC rate was 18.8%, with a survival rate of 7.2% at hospital discharge. Among the 50 patients who underwent a US examination of HPV, 40 showed HPVG. The HPVG group had a significantly lower ROSC rate and survival rate at ED discharge and hospital discharge compared to the group without HPVG. Conclusion. Traumatic OHCA with HPVG presents a significantly worse prognosis. This suggests that early consideration of termination or withholding of CPR may be appropriate in such cases.
Predictive Value of Red Cell Distribution Width‐to‐Platelet Ratio Combined with Procalcitonin in 28‐day Mortality for Patients with Sepsis
Authors: Si Ying , Sun Bo , Huang Yongmao , Xiao Ke
Published: August 2024

Objectives. The objective of this study was to investigate the predictive value of erythrocyte distribution width‐to‐platelet ratio (RPR) combined with procalcitonin (PCT) on 28‐day mortality in patients with sepsis. Methods. A total of 193 patients with sepsis admitted to the Affiliated Hospital of Southwest Medical University from January 2013 to January 2018 were selected as the study objects. Univariate and multivariate analyses were used to understand the indicators related to the 28‐day prognosis of patients, and the ROC curve was further drawn. The Kaplan–Meier curve was used to evaluate the prognosis of patients. Results. A total of 193 patients were enrolled and divided into the survivor group (=156) and nonsurvivor group (=37) according to the prognosis within 28 days. The median age was 62.5 years, and 64.7% were males. Multivariate analysis showed that PCT and RPR were independent risk factors for 28‐day prognosis in sepsis patients. The area under the ROC curve of PCT and RPR were 0.894 and 0.861, respectively, and the cutoff values were 27.04 and 0.12, respectively. Survival curve analysis showed that PCT and RPR were associated with the 28‐day prognosis of patients, and the combination of PCT and RPR had a better predictive effect. Conclusions. PCT and RPR are independent predictors of sepsis prognosis. The combined application of PCT and RPR (PCT‐RPR) can further improve the predictive performance and provide a reference for the clinical diagnosis, treatment, and prognosis evaluation of sepsis patients.
Supplementary Figure 3 from IFNγ Production by Functionally Reprogrammed Tregs Promotes Antitumor Efficacy of OX40/CD137 Bispecific Agonist Therapy
Authors: Imianowski Charlotte J. , Kuo Paula , Whiteside Sarah K. , von Linde Teresa , Wesolowski Alexander J. , Conti Alberto G. , Evans Alexander C. , Baird Tarrion , Morris Benjamin I. , Fletcher Nicole E. , Yang Jie , Poon Edmund , Lakins Matthew A. , Yamamoto Masahiro , Brewis Neil , Morrow Michelle , Roychoudhuri Rahul
Published: August 2024

Supplementary Figure 3: Increased IL-2 expression by conventional CD4+ and CD8+ T cells with FS120m treatment. Representative plots (left) and replicate measurements (right) of IL-2+ CD4+ Tconv cells and CD8+ T cells in the spleen and draining lymph nodes on day 18 post tumor implantation. *** P ≤ 0.001, **** P ≤ 0.0001. Unpaired Student’s t test. Bars and error are mean and s.e.m.
Supplementary Figure 1 from IFNγ Production by Functionally Reprogrammed Tregs Promotes Antitumor Efficacy of OX40/CD137 Bispecific Agonist Therapy
Authors: Imianowski Charlotte J. , Kuo Paula , Whiteside Sarah K. , von Linde Teresa , Wesolowski Alexander J. , Conti Alberto G. , Evans Alexander C. , Baird Tarrion , Morris Benjamin I. , Fletcher Nicole E. , Yang Jie , Poon Edmund , Lakins Matthew A. , Yamamoto Masahiro , Brewis Neil , Morrow Michelle , Roychoudhuri Rahul
Published: August 2024

Supplementary Figure 1: OX40 and CD137 are highly expressed on Treg cells. Representative plots showing OX40+ (left) and CD137+ (right) SP resting or activated cells from the spleen and tumor on day 18 post tumor implantation. ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. One-way ANOVA with Tukey’s correction for multiple comparisons. Bars and error are mean and s.e.m.
Figure 6 from IFNγ Production by Functionally Reprogrammed Tregs Promotes Antitumor Efficacy of OX40/CD137 Bispecific Agonist Therapy
Authors: Imianowski Charlotte J. , Kuo Paula , Whiteside Sarah K. , von Linde Teresa , Wesolowski Alexander J. , Conti Alberto G. , Evans Alexander C. , Baird Tarrion , Morris Benjamin I. , Fletcher Nicole E. , Yang Jie , Poon Edmund , Lakins Matthew A. , Yamamoto Masahiro , Brewis Neil , Morrow Michelle , Roychoudhuri Rahul
Published: August 2024

The antitumor efficacy of OX40/CD137 dual agonism is partially dependent upon IFNγ production by Tregs and/or their lineage-instable progeny. A, PCR genotyping of CD8+, CD4+ Foxp3-GFP− Tconv, and CD4+ Foxp3-GFP+ Tregs sorted from the spleens of mice with the indicated genotypes after treatment with tamoxifen. Tregs have cell-specific excision of IFNγ only in mice possessing both the Ifngfl/fl and Foxp3EGFP-Cre-ERT2 alleles. B, Schema representing treatment schedule of tamoxifen and FS120m. C, Tumor measurements at indicated timepoints after MC38 implantation of Ifngfl/flFoxp3EGFP-Cre-ERT2 mice and controls given the indicated treatment. D, Replicate measurements of IFNγ+ cells in the indicated CD4+ T-cell populations from the tumor. E, Percentages (top) and counts per gram (bottom) of the indicated cell types in the tumors of mice of the indicated genotypes given the specified treatment. Data were analyzed by two-way ANOVA with Tukey correction for multiple comparisons (C) and ordinary one-way ANOVA with Tukey correction for multiple comparisons (D and E). Bars and error are mean and SEM. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Ctrl, control.
Video S5 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Automatic cell tracking before the AI model is applied. TMS cell line, a well without the addition of inhibitors on a Poly-L-lysine matrix.
Video S3 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Motility of Hsp70-positive cells emerging from a piece of tumor during cultivation for three days.
Figure 2 from IFNγ Production by Functionally Reprogrammed Tregs Promotes Antitumor Efficacy of OX40/CD137 Bispecific Agonist Therapy
Authors: Imianowski Charlotte J. , Kuo Paula , Whiteside Sarah K. , von Linde Teresa , Wesolowski Alexander J. , Conti Alberto G. , Evans Alexander C. , Baird Tarrion , Morris Benjamin I. , Fletcher Nicole E. , Yang Jie , Poon Edmund , Lakins Matthew A. , Yamamoto Masahiro , Brewis Neil , Morrow Michelle , Roychoudhuri Rahul
Published: August 2024

OX40/CD137 bispecific agonist (FS120m) drives functional fragility and lineage instability of Tregs. A, Schema (top) showing tamoxifen and FS120m treatment schedule. Tumor measurements (bottom) at indicated timepoints after MC38 tumor implantation. (Solid line, mean values; dotted lines, individual mouse tumor curves.) B, Representative plots showing percentages of CD4+ GFP+ RFP+ Tregs and CD4+ RFP+ GFP− exTregs of total CD4+ T cells in the spleens and MC38 tumors of Foxp3EGFP-Cre-ERT2RosaflSTOPfl-RFP reporter mice after treatment. C, Quantification of the percentage of RFP+ single-positive exTregs (GFP−) out of total RFP+ cells in spleens and tumors. D, Representative plots showing the production of IFNγ and TNF by indicated cell subsets from the spleens and tumors of reporter mice. E, Quantification of IFNγ and TNF production by indicated cell types from the spleens and tumors of MC38 tumor–bearing mice at day 21 after tumor implantation. F, Schema showing setup of Treg suppression assay (left), representative flow plots (center), and replicate measurements (right) showing percentage of dividing responders in indicated conditions. Data were analyzed by two-way ANOVA with Šídák correction for multiple comparisons (A), unpaired Student t test (C), with Bonferroni–Dunn correction for multiple comparisons (E), and ordinary one-way ANOVA with Tukey correction for multiple comparisons (F). Bars and error are mean and SEM. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. Ctrl, control.
Video S2 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Motility of Hsp70-positive cells in a GBM tumor sample detected using laser scanning confocal microscopy during observation for 1 h, zoomed field of view from the center of the Video S1 frame.
Table 1 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Patients and tumor characteristics
Supplementary Table S5 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

List of proteins detected by mass spectrometric analysis of lipid rafts isolated from normal brain tissue (obtained from a patient with epilepsy).
Supplementary Table S1 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Characteristics of adult patients with high-grade gliomas.
Supplementary Figure S5 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Evaluation of biomarkers co-localization.
Supplementary Figure S4 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

The sequence of constructing a phenotyping map for histological sections of human GBM.
Supplementary Figure S1 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Gating of mHsp70High cells (field R2) and mHsp70Low cells (field R3) by FACS.
Figure 3 from Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors
Authors: Shevtsov Maxim , Bobkov Danila , Yudintceva Natalia , Likhomanova Ruslana , Kim Alexander , Fedorov Evegeniy , Fedorov Viacheslav , Mikhailova Natalia , Oganesyan Elena , Shabelnikov Sergey , Rozanov Oleg , Garaev Timur , Aksenov Nikolay , Shatrova Alla , Ten Artem , Nechaeva Anastasiya , Goncharova Daria , Ziganshin Rustam , Lukacheva Anastasiya , Sitovskaya Daria , Ulitin Alexey , Pitkin Emil , Samochernykh Konstantin , Shlyakhto Evgeny , Combs Stephanie E.
Published: August 2024

Mass spectrometry analysis of the tumor-derived lipid rafts. A, Designation of the brain tumor regions on postcontrast T1-weighted image for obtaining tumor samples from necrotic, tumor, and peritumoral regions. B, Representative confocal microscopy images of the brain tumor samples obtained from three regions and stained for mHsp70 (green) and DAPI (blue) for nuclei. Scale bars, 100 μm. C–E, Analysis of the mass spectrometry data from isolated lipid rafts. Protein functional groups identified using the STRING database in the proteome of lipid rafts from three tumor zones (Supplementary Fig. S7). C, A subnetwork of highly membrane-associated proteins isolated from the tumor-derived lipid raft and D, normal tissue-derived lipid raft STRING PPI proteome network, separated into clusters using the Markov cluster algorithm method. Color mapping (light to dark) was done to indicate the nodes belonging to the cytoskeletal compartment, the size of the node corresponding to the protein abundance index obtained from mass spectrometry data. E, The cluster containing highly plasma membrane-associated proteins contains simultaneously heat shock proteins, small GTPases, and cytoskeletal proteins.
Hairbrushes: A Guide for Dermatologists
Authors: Williams Kimberly N. , Lee Juwon , Robles Esthefani , Tosti Antonella
Published: August 2024

Background: Hairbrushes play an integral role in our day-to-day life. As dermatologists, it can be difficult to keep up with this constant influx of new and unique brush types. This article provides a summary of the ten most commonly used hair brushes all dermatologists should know, focusing on their design, suitable hair types, and proper usage. Summary: There are a wide variety of hairbrushes on the market today ranging from detangling brushes to boar bristle brushes. Improper use of these hairbrushes can lead to trichomalacia, trichorrhexis nodosa, and traction alopecia. This guide aims to summarize the most common types of hairbrushes and their proper use for dermatologists. Key Messages: Improper use of hairbrushes can lead to trichomalacia, trichorrhexis nodosa, and traction alopecia. Through this guide, we hope to provide helpful information on hairbrushes that can be utilized daily in clinical practice for hair types ranging from straight to coily.
Sex Disparity in the In-Hospital Outcomes of Patients with Kidney Disease Admitted for Myocardial Infarction: Insights from a Large National Database
Authors: Rivera Frederick Berro , Ruyeras Jade Monica Marie , Salva Wailea Faye C. , Balbin Jeremiahdominic , Tang Samantha , Pine Polyn Luz S. , Tangco Gabriel A. , Bantayan Nathan Ross B. , Amigo John Andrew C. , Ansay Marie Francesca M. , Matabang Maria Angela , Lerma Edgar V. , Ong Kenneth , Collado Fareed Moses , Kazory Amir
Published: August 2024

Introduction: There is limited evidence as to the effect of sex on the outcomes of patients admitted for ST-elevation myocardial infarction (STEMI) who have a concomitant diagnosis of chronic kidney disease (CKD) and end-stage renal disease (ESRD). We aimed to determine if there are differences in the outcomes between males and females in these patient populations. Methods: Data were obtained from the National Inpatient Sample database and patients were selected using the International Classification of Diseases, Ninth and Tenth Revision (ICD-9 and -10) codes. Hospitalizations for patients with CKD who had STEMI from 2012 to 2020 were included. The primary outcome of interest was in-hospital mortality. Secondary outcomes evaluated included ischemic stroke, major bleeding complications, pressor requirement, permanent pacemaker implantation, percutaneous coronary intervention, coronary artery bypass grafting, surgery, pericardiocentesis, mechanical circulatory support, and mechanical ventilation. Results: A total of 1,283,255 STEMI patients without CKD, 158,715 STEMI patients with CKD, and 22,690 STEMI patients with ESRD were identified and analyzed. Among patients with STEMI and CKD, females demonstrated higher in-hospital mortality compared to male counterparts (16.7% vs. 12.7%, aOR = 1.13, 95% CI: 1.05–1.21, p < 0.01). While there was no sex difference in the in-hospital mortality among STEMI patients with ESRD, female patients in this group were less likely to receive coronary artery bypass grafting and mechanical circulatory support. Conclusion: Increased in-hospital mortality rates were shown for females admitted for STEMI with CKD. Among patients with ESRD who had STEMI, females were less likely to receive coronary artery bypass grafting and mechanical circulatory support. Further research needs to be conducted to better explain this said difference in outcomes.
The Harp and the Eagle: teaching Irish Poetry in Mexico
Authors: Murgia Mario
Published: August 2024

Ireland and Mexico share a long tradition of intercultural relationships. The Latin American nation has received significant influence from the mind-frames and oeuvre of Irish or Irish-descended thinkers, and authors. In the field of literature, the presence of Irish writers in Mexico has been equally relevant. A number of them are constantly referenced in middle- to higher-education institutions as paradigmatic examples of the Anglophone belles lettres. Nevertheless, and with the possible exception of Yeats, limited academic and pedagogic attention has been paid to Irish poetry, almost exclusively in English, until comparatively recent times. As of the mid-2000s, the School of Philosophy and Literature (FFyL) of the National Autonomous University of Mexico (UNAM) has witnessed a surge in academic efforts to promote the verse production of Anglo-Irish poets in the country. With the publication in 2003 of the anthology Una lengua injertada [A Grafted Tongue] and, more recently, with the establishment of the Contemporary Anglo-Irish Literature Research Project, FFyL-UNAM have inaugurated a new era for the study of (Anglo-) Irish verse in Mexico and Spanish-speaking America. This article will explore the critical and pedagogical approaches with which FFyL-UNAM have tackled the teaching of (Anglo) Irish poetry over at least one decade.
(Im)possible Facilitating Environments: the Spaces of Family in Sally Rooney’s Normal People
Authors: Angelo Bárbara Moreira Bom
Published: August 2024

Building upon the concept of the “good enough” facilitating environment developed by pediatrician and psychoanalyst D. W. Winnicott, this article aims to analyze the family relationships depicted in the novel Normal People by Sally Rooney. Through the contrast of experiences, the author develops two distinct family environments — for Connell Waldron, the space is nurturing, while for Marianne Sheridan, it is marked by violence. This narrative choice allows for reflections on the impacts of these spaces on the emancipation process of the protagonists. They initiate a romantic relationship in their final year of high school and, amidst ups and downs, remain intertwined in each other's lives until the end of college, when they must navigate separate paths. Through the dialogues between the protagonists and the information provided by the third-person narrator, which alternates between the two points of view, this article aims to analyze how the family dynamics of each character influence their self-perception and the choices they make over the four-year period covered in the novel.
Abstract 5079: Predictive Accuracy of Cardiopulmonary Exercise Stress Testing in Subjects With Chest Pain
Authors: Belardinelli Romualdo , Lacalaprice Francesca , Mucaj Andi , Littarru Giampaolo
Published: August 2024

Cardiopulmonary exercise testing (CPET) is widely used to grade the severity of heart failure and to assess its prognosis. However, it is unknown whether CPET may identify coronary artery disease (CAD) or exclude it in patients with chest pain. We prospectively studied 1,265 consecutive patients (55±8 years, 156 women, 628 retired from work, 234 white collar, 244 blue collar) who were evaluated with ECG stress testing (TE) for chest pain. No one had a previous myocardial infarction or documented CAD. All patients performed an incremental CPET on an electronically-braked cycle ergometer (Ergometrics 800S) until volitional fatigue. ECG was recorded during the test. Cardiovascular risk factors were present in 65%. Criteria for a positive CPET were: flattening in VO2/work rate slope with an inflection point more than 1 minute earlier than peak exercise and a flattened slope from the inflection point to peak exercise (<4 ml/min/W); the time from the inflection point to peak exercise should be similar in O2pulse. These two last criteria have been recently validated by our group in 202 patients with documented CAD (

R.Belardinelli et al,

Eur Heart J

,
2003
;
24
:
1285

). Follow-up lasted 12 months. Of 1,265 patients, 73 had a positive CPET and 1,192 had a negative CPET. Cardiac events occurred in 32 patients with a positive CPET and 10 out of 1,192 (positive predictive accuracy(PPV): 76%; negative predictive accuracy (NPV): 95%). Coronary angiography was performed in 73 patients with a positive CPET and revealed a CAD in 64. As compared with ET, sensitivity, specificity, PPV and NPV were all improved (ET: 48%,55%,33%,95%; CPET: 88%, 98%, 73%,99%, respectively). Patients with a peak VO2≥91% of predicted VO2max had no CAD in 100% of cases. In conclusion, CPET shows a predictive accuracy for CAD greater than TE in patients with chest pain. Its use should be encouraged among physicians in clinical practice.
Abstract 1627: Molecular Substrates of Carotid Body Chemoreceptor Activation
Authors: Tan Zhi-Yong , Lu Yongjun , Whiteis Carol A , Chapleau Mark W , Abboud Francois M
Published: August 2024

Carotid bodies are powerful regulators of sympathetic nerve activity in response to acidosis, hypoxia and hypercarbia. They are hypersensitive in heart failure and hypertension. The molecular determinants of their responsiveness and hypersensitivity are not fully understood. We recently reported that the acid sensitive channels ASICs and TASKs mediate early and late depolarizations, respectively, in isolated glomus cells (

Circ. Res.

2007
;
101
:
1009
–19

). In these studies we addressed two questions. The first is whether the enhanced carotid body hypersensitivity known to occur in spontaneously hypertensive rats (SHR) reflects enhanced activity of ASICs and TASKs. The results indicate that low pH ranging from 7.0 to 6.0 induced both an early rapid and a late sustained depolarization of glomus cells isolated from young (4 – 6 weeks) SHR and WKY rats, however the response was markedly enhanced in SHR. The values for rapid and sustained depolarization at pH 6.0 were 24.4 ± 3.7 and 39.2 ± 2.3 mV for SHR cells (n = 16), and 12.7 ± 2.3 and 29.5 ± 2.6 mV for WKY cells (n = 26), respectively. The ASIC blocker amiloride (200 μM) suppressed significantly the early depolarization to a level of 2.0 ± 1.2 mV (n = 7), but not the sustained depolarization which remained at 33.6 ± 3.6 mV (n = 7). mRNAs encoding ASIC1b and 3 and TASK1 and 3 were augmented by 150%, 75%, 100% and 20% respectively in carotid bodies of SHR compared to WKY. The second question is whether glomus cells are selectively responsive to low pH vs. oxygen deprivation with cyanide. In contrast to the response to low pH, that to cyanide (2 mM) was not enhanced in SHR (13.9 ± 2.5 mV; n = 7) compared to WKY (11.8 ± 3.3 mV; n = 7). Moreover in six WKY cells, three were as sensitive to cyanide (20.0 ± 3.9 mV) as they were to low pH (29.3 ± 4.6 mV) whereas three other cells that were very sensitive to low pH (29.2 ± 1.9 mV) had negligible responses to cyanide (4.2 ± 0.9 mV).

We conclude:

that in a genetic model of neurogenic hypertension (SHR), overexpression of acid sensitive ion channels (ASICs and TASKs) causes selective carotid body chemoreceptor hypersensitivity to low pH and may contribute to the development of hypertension; and

that different glomus cell populations may be selectively sensitive to specific chemical stimuli (e.g. low pH vs. hypoxia).
Abstract 1629: Adrenal-Targeted GRK2 Gene Deletion Ameliorates Sympathetic Overstimulation and Improves Function of the Failing Heart
Authors: Lymperopoulos Anastasios , Rengo Giuseppe , Gao Erhe , Moraca Susan R , Ebert Steven N , Dorn Gerald W , Koch Walter J
Published: August 2024

Chronic heart failure (HF) is characterized by sympathetic overactivity reflected by enhanced circulating catecholamines (CAs), which contribute to increased morbidity and mortality. We recently reported that adrenal G-protein coupled receptor kinase-2 (GRK2) is up-regulated in chronic HF, leading to enhanced CA release via desensitization/downregulation of the chromaffin cell a
2
-adrenergic receptors (a
2
ARs) that normally inhibit CA secretion. We also showed that adrenal GRK2 inhibition via adenoviral-mediated in vivo gene therapy acutely decreases circulating CAs and improves cardiac inotropic reserve and function (

Lymperopoulos, A. et al.,

Nat. Med.

13
:
315
–323,
2007

). In the present study, we hypothesized that adrenal-targeted GRK2 gene deletion might be beneficial in chronic HF by means of normalizing sympathetic activity.

Methods:
To specifically delete GRK2 only in the chromaffin cells of the adrenal gland, we utilized the Cre/loxP technology, crossing PNMT-Cre mice, which express Cre recombinase under the gene promoter of phenylethanolamine N-methyl transferase (PNMT), a chromaffin cell-specific enzyme. We crossed these mice with floxGRK2
+/+
mice, which have the GRK2 gene flanked by loxP sites, thus targeted for deletion by Cre. At 2 months of age, offspring underwent surgical myocardial infarction (MI) to induce HF, and 4 weeks later, cardiac function, adrenal physiology and CA levels were assessed.

Results:
Genetic screening and western blotting in adrenal extracts confirmed a significant (54 ± 10 % vs. control, non-crossed floxGRK2
+/+
) reduction of adrenal GRK2 protein levels in PNMTCre/floxGRK2 mice. Adrenal weight-to-body weight ratio was also significantly lower at 4 weeks post-MI, indicating prevention of post-MI adrenal hypertrophy. Additionally, plasma circulating CAs were reduced, and, importantly, cardiac ejection fraction of PNMTCre/floxGRK2 mice was markedly higher than that of floxGRK2
+/+
mice at 4-weeks post-MI (39.6 ± 1.0%, n = 9, vs. 30.9 ± 1.6%, n = 11, respectively, p = 0.0005). Moreover, contractile function and βAR signaling significantly improved.

Conclusions:
Adrenal-targeted GRK2 gene knockout decreases circulating CAs, leading to improved cardiac inotropic/adrenergic reserve in post-MI HF.

This research has received full or partial funding support from the American Heart Association, AHA Great Rivers Affiliate (Delaware, Kentucky, Ohio, Pennsylvania & West Virginia).
Abstract P29: The Efficacy of Emergency Coronary Reperfusion Therapy for Comatose Survivors After Out-of-hospital Cardiac Arrest is Equivalent to Cardiogenic Shock Complicating ST-elevation Myocardial Infarction
Authors: Tachibana Eizo , Nagao Ken , Kikushima Kimio , Takayama Tadateru , Satoh Naoki , Yamada Kyoushi , Takayama Morimasa
Published: August 2024

BACKGROUNDS: AHA guidelines for ST-elevation myocardial infarction (STEMI) have recommended early coronary revascularization for patients with cardiogenic shock complicating STEMI (

Circulation

2004
;
110
:
586
– 636

). ILCOR has suggested that STEMI patients with post-cardiac arrest should undergo emergency coronary angiography, with subsequent reperfusion therapy (

Circulation

2008
;
118
:
2452
–2483

). We investigated whether early coronary reperfusion therapy had survival benefits for STEMI patients with post-cardiac arrest.

METHODS: We did a multicenter observation study of STEMI patients. Of those patients, comatose survivor or cardiogenic shock were enrolled in this study. The primary endpoint was survival to hospital discharge.

RESULTS: A total of 3,633 STEMI patients were admitted to the CCU in the Tokyo CCU network. Of those, 249 were enrolled in this study (88 in comatose survivors after out-of-hospital cardiac arrest and 161 in cardiogenic shock). Emergency coronary reperfusion therapy was performed 80 % in the cardiac arrest(CA) group, and 78% in the shock group (p=0.67). In the overall survival rate, no significant difference was seen between the CA-group and the Shock-group (66 % vs. 60%; p=0.32). However, the survival rates were significant higher in each group of patients treated with reperfusion therapy than no-reperfusion therapy (the CA-group: 77% vs. 25%, P<0.01, the Shock-group: 69% vs. 29%, P<0.01). And no significant difference was seen between the CA-group and the Shock-group in the survival rate of the patients who underwent reperfusion therapy (the CA-group: 77% vs. 69%, P=0.3).

CONCLUSIONS: Emergency coronary reperfusion therapy improved the survival in STEMI patients with not only cardiogenic shock but also out-of-hospital cardiac arrest, and the two groups had similar survival rate at about 70%.

This research has received full or partial funding support from the American Heart Association, National Center.
Abstract 544: Prognostic Implications of Left Ventricular Mass and Geometry Following a First ST-Elevation Acute Myocardial Infarction
Authors: Iwahashi Noriaki , Kosuge Masami , Okuda Jun , Tsukahara Kengo , Tahara Yoshio , Hibi KIyoshi , Ebina Toshiaki , Sumita Shinichi , Ishikawa Toshiaki , Uchino Kazuaki , Umemura Satoshi , Umemura Satoshi , Kimura Kazuo
Published: August 2024

Left ventricular (LV) hypertrophy and alterations in LV geometry (Concentric LV hypertrophy and higher relative wall thickness: RWT) are associated with an increased risk of adverse events in a high risk acute myocardial infarction (AMI) from VALIANT echocardiography study (

JACC Img

2008
;
1
:
582

). However, the role of LV hypertrophy and RWT on a first ST-elevation AMI (STEMI) remains unclear. The aim of our study was to explore the impact of LV hypertrophy and geometry on a first reperfused STEMI exclusively involved. 278 consecutive STEMI patients (age = 64 ± 12years, male = 222, LMT = 5, LAD = 126, RCA = 115, LCx = 32) underwent echocardiography 2 weeks later and were followed for 36months. They underwent PCI successfully within 12 hours of onset. Patients with non-STEMI, A–C bypass, atrial fibrillation, and chronic renal failure on dialysis were excluded. LV mass index (LVMi) and RWT were calculated. Patients were classified into 4 mutually exclusive groups based on RWT and LVMi as follows: normal geometry (normal LVMi and normal RWT), concentric remodeling (normal LVMi and increased RWT), eccentric hypertrophy (increased LVMi and normal RWT), and concentric hypertrophy (increased LVMi and increased RWT). A cut-off value of LVMi was defined as 130g/m2 and RWT as 0.45. Primary end point was a major adverse cardiac/cerebrovascular event (MACCE: death from cardiovascular disease, ACS, readmission because of heart failure, stroke). MACCE was occurred in 49 patients within 36months (2 death, 21heart failure,18 ACS, 5 stroke, 3 arrhythmia). Eccentric hypertrophy was only an independent predictor of deterioration of renal function (eGFR decreased from baseline >20%) (Odds ratio=4.12, 95%CI: 1.81–10.8, p<0.01) and MACCE (Odds Ratio=2.99, 95% CI: 1.23 to 7.27, p<0.01). This might be explained by the fact that eccentric hypertrophy stimulates renin-angiotensin-aldosterone system in LV remodeling strongly. RWT did not associate with deterioration of renal function or MACCE (Odds ratio=0.23, 0.31). Eccentric LV hypertrophy carries the greatest risk of MACCE among a first STEMI. RWT did not associate with an increased risk of cardiovascular complications among the patients with a first reperfused STEMI successfully.
Abstract 1364: Impact of the ApoB/A1-Ratio on the Prediction of the Elevation of Left Ventricular Filling Pressure After a First STEMI
Authors: Iwahashi Noriaki , Kosuge Masami , Okuda Jun , Tsukahara Kengo , Tahara Yoshio , Tahara Yoshio , Hibi Kiyoshi , Ebina Toshiaki , Sumita Shinichi , Ishikawa Toshiaki , Uchino Kazuaki , Umemura Satoshi , Kimura Kazuo
Published: August 2024

Background
The non-fasting ApoB/ApoA1-ratio was superior to any of the cholesterol ratios for estimation of the risk of acute myocardial infarction (AMI) (

Lancet

2008
;
32
.
224

). The AMORIS study prospectively revealed that the strongest single lipoprotein-related risk factor for fatal AMI was the Apo-B/Apo-A-I ratio in a large cohort by 10.3 years follow up. (

AJC

2006
;
97
.
243

). The aim of this study was to explore the impact of ApoB/A1 on the damage to cardiac function after a first ST-elevation AMI (STEMI).

Methods
241 consecutive patients (age=63.8±11.9 years, male=197 patients, culprit lesions: LMT=3, LAD=109, RCA=103, LCx=26) underwent successful emergent PCI within 12 hours of onset. Blood sampling, including apolipoproteins, was obtained at their emergent admission. They underwent echocardiography 2 weeks after onset. We examined the tissue Doppler imaging, then calculated the early transmitral flow velocity (E) divided by the early diastolic velocity of the mitral valve annulus (e′) - a ratio that strongly correlates with mean left ventricular filling pressure. Exclusion criteria were those with chronic kidney disease (CKD) at Stage 4 and 5 (eGFR <30 ml/min), anemia (Hemoglobin<10g/dl), significant valvular heart disease, prior MI, non-STEMI, A-C bypass and atrial fibrillation. Patients were followed up for 36 months.

Results
There were significant relationships between the Apo-B/Apo-A-1 ratio and E/e′ (r=0.20, p<0.001) and between Apo-B and E/e′ (r=0.15, p<0.05). However, the other apolipoproteins and conventional lipid profiles did not show associations with echocardiographical parameters, including E/e′. Furthermore, the Apo-B/Apo-A-1 ratio was the most powerful predictor of the progression of CKD (when eGFR decreased from baseline >20%) after 36 months(Odds ratio=3.55,95%CI:1.22–10.37).

Conclusion
The value of the Apo-B/A-1-ratio on admission could predict the elevation of LV filling pressure 2 weeks after a first STEMI onset. The higher the Apo-B/A-1-ratio on admission, the greater the LV damage from STEMI, despite patients having successfully received early reperfusion therapy. These results might explain why the higher Apo-B/Apo-A-I ratio is frequently followed by fatal AMI.