bluuebunny/crossref_metadata_2025 huggingface dataset:
A novel methodology for a time-delayed controller to prevent nonlinear system oscillations
Authors: Moatimid Galal M , Amer TS , Ellabban YY
Published: August 2023

The paper investigates the nonlinear transversal vibrations of a cantilever beam structure in the primary resonance case. A time-delayed position-velocity control is suggested to reduce the nonlinear vibrations of the structure under consideration. A non-perturbative method (NPM) is used to get an equivalent analogous linear differential equation (DE) to the original nonlinear one. For the benefit of the readers, a comprehensive description of the NPM method is provided. The theoretical findings are validated through a numerical comparison carried out by employed the Mathematica Software. Both the numerical solutions and the theoretical outcomes showed excellent agreement. As well-known, all classic perturbation techniques use Taylor expansion, when the restoring forces are present, to expand these forces and therefore lessen the difficulty of the given problem. Under the NPM, this weakness is no longer present. Furthermore, one may examine the stability examination of the issue with the NPM something that was not possible with prior traditional techniques. The controlled linear equivalent model is examined using the multiple-scales homotopy method. The amplitude-phase modulation equations which control the dynamics of the structure at the various resonance circumstances are established. The loop-delay stability diagrams are analyzed. It is looked at how the different controller parameters impact the oscillation behaviors of the system. The obtained theoretical outcomes showed that the loop delay has an important impact on the effectiveness of the control. Therefore, the ideal loop-delay values are given and used to develop the enactment of the organized control. The completed analytical results are also numerically validated, to reveal their good correlation with the achieved theoretical new results.
The Limits of Counterculture Urbanism: Utopian Planning and Practical Politics in Berkeley, 1969–73
Authors: Raynsford Anthony
Published: August 2023

Around 1970, the City of Berkeley briefly became an epicenter of radical experimentation in urban planning and design, directly stemming from the counterculture of the late 1960s. This essay examines the ideological and political emergence of Berkeley’s counterculture urbanism, arguing that its experiments left two important legacies in the history of planning. On the level of utopian thought, it articulated a clear alternative to mainstream capitalist urban development, or what Henri Lefebvre called “abstract space.” On the level of contemporary planning practices, it opened up still-unresolved conflicts, especially between localized environmental preservation and the abstract, economic demands for affordable housing.
Stakeholders’ experiences of ethical challenges in cluster randomized trials in a limited resource setting: a qualitative analysis
Authors: Mtande Tiwonge K , Lombard Carl , Nair Gonasagrie , Rennie Stuart
Published: August 2023

Although the use of the cluster randomized trial (CRT) design to evaluate vaccines, public health interventions or health systems is increasing, the ethical issues posed by the design are not adequately addressed, especially in low- and middle-income country settings (LMICs). To help reveal ethical challenges, qualitative interviews were conducted with key stakeholders experienced in designing and conducting two selected CRTs in Malawi. The 18 interviewed stakeholders included investigators, clinicians, nurses, data management personnel and community workers who were invited to share their experiences related to implementation of CRTs. Data analysis revealed five major themes with ethical implications: (1) The moral obligation for health care providers to participate in health research and its compensation; (2) Suboptimal care services compromising the integrity of CRT; (3) Ensuring scientific validity and withholding care service; (4) Obtaining valid consent and permission for waiver of consent; and (5) Inadequate risk assessment for trial participation. Understanding key ethical issues posed by CRTs in Malawi could improve ethical review and research oversight of this particular study design.
Promoting angiogenesis and diabetic wound healing through delivery of protein transduction domain-BMP2 formulated nanoparticles with hydrogel
Authors: Suh Jae Wan , Lee Kyoung-Mi , Ko Eun Ae , Yoon Dong Suk , Park Kwang Hwan , Kim Hyun Sil , Yook Jong In , Kim Nam Hee , Lee Jin Woo
Published: August 2023

Decreased angiogenesis contributes to delayed wound healing in diabetic patients. Recombinant human bone morphogenetic protein-2 (rhBMP2) has also been demonstrated to promote angiogenesis. However, the short half-lives of soluble growth factors, including rhBMP2, limit their use in wound-healing applications. To address this limitation, we propose a novel delivery model using a protein transduction domain (PTD) formulated in a lipid nanoparticle (LNP). We aimed to determine whether a gelatin hydrogel dressing loaded with LNP-formulated PTD-BMP2 (LNP-PTD-BMP2) could enhance the angiogenic function of BMP2 and improve diabetic wound healing. In vitro, compared to the control and rhBMP2, LNP-PTD-BMP2 induced greater tube formation in human umbilical vein endothelial cells and increased the cell recruitment capacity of HaCaT cells. We inflicted large, full-thickness back skin wounds on streptozotocin-induced diabetic mice and applied gelatin hydrogel (GH) cross-linked by microbial transglutaminase containing rhBMP2, LNP-PTD-BMP2, or a control to these wounds. Wounds treated with LNP-PTD-BMP2-loaded GH exhibited enhanced wound closure, increased re-epithelialization rates, and higher collagen deposition than those with other treatments. Moreover, LNP-PTD-BMP2-loaded GH treatment resulted in more CD31- and α-SMA-positive cells, indicating greater neovascularization capacity than rhBMP2-loaded GH or GH treatments alone. Furthermore, in vivo near-infrared fluorescence revealed that LNP-PTD-BMP2 has a longer half-life than rhBMP2 and that BMP2 localizes around wounds. In conclusion, LNP-PTD-BMP2-loaded GH is a viable treatment option for diabetic wounds.
Timing of Complications Following Surgery for Distal Femur Fractures in Older Adults
Authors: Onizuka Naoko , Farmer Samuel , Wiseman Jessica M. , Alain Gabriel , Quatman-Yates Catherine C. , Quatman Carmen E.
Published: August 2023

Introduction

The purpose of this study was to identify the timing and nature of complications associated with distal femur fracture surgery in patients aged 65 and older using the American College of Surgeons National Surgical Quality Improvement Program (ACS NSQIP) database.

Methods

The ACS NSQIP database was queried for adults aged 65 and older who received surgical treatment for a distal femur fracture between 01 January 2015 and 31 December 2021. Cox regression models and risk tables adjusted for baseline clinical characteristics were created for 14 complications (Superficial Surgical Site Infection (SSI), Deep SSI, Organ/Space SSI, Pneumonia, Pulmonary Embolism (PE), Deep Venous Thrombosis (DVT), Urinary Tract Infection (UTI), Stroke/Cerebrovascular accident (CVA), Myocardial Infarction (MI), Renal Failure, Cardiac Arrest (CA), Re-operation, Sepsis, and Death within 30 days of surgery). Model summaries were used to identify significant variables with a Bonferroni correction applied.

Results

A total of 3956 adults met inclusion criteria and were included in analysis. The most common complications were UTI (5.2%), death (4.1%), and pneumonia (3.4%). Complications typically occurred within 14 days after surgery, except for SSI, which occurred between post-op days 11 and 24.

Conclusions

Distal femur fractures are a substantial source of morbidity and mortality in the older adult population. Our findings underscore the need for comprehensive preoperative risk assessment and patient management strategies to mitigate the impact of identified risk factors in this vulnerable population.
A Comprehensive Analysis on the Readability of Rhinoplasty-Based Web Content for Patients
Authors: Levine Emma C. , McGee Shayan A. , Kohan Joshua , Fanning James , Willson Thomas D.
Published: August 2023

Background: Rhinoplasty is one of the most common cosmetic procedures performed by plastic surgeons and otolaryngologists. Previous studies have concluded that the readability of rhinoplasty information does not meet the recommended guidelines for the 8th grade reading level. In this study, we performed an updated readability analysis and a comprehensive online review of decision-making factors for patients considering rhinoplasty. Additionally, the differences between plastic surgery and otolaryngology-specific web pages were also analyzed in this study. Methods: This study analyzed 30 websites from universities and private practice webpages for decision-making factors, readability, and tone. These sites were identified by performing a depersonalized Google search using the search terms “nose job decision factors” and “rhinoplasty decision factors.” An independent t-test was performed to assess the differences between webpages. Results: A total of 30 websites were comprehensively reviewed for a comparative analysis of readability for potential rhinoplasty patients. Decision-making factors were divided into 3 categories: individual factors, goals of the procedure, and technical considerations. These categories were then further divided into subcategories. The most common decision-making factor discussed was procedural considerations (83.3%), the least common being lifestyle (10%). Additionally, only 26.7% of web pages mentioned ethnic background and skin type as potential considerations before surgery. Less than half (40%) discussed the importance of setting realistic expectations for the procedure. Of the total sites analyzed in the study, 65.5% were ENT-based, 24.1% were plastic surgery-based, 3.4% were dental/oral maxillofacial surgeons, and 6.9% were combined plastic surgeons and ENT. Statistically significant differences between ENT- and plastic surgeon-based webpages were observed. Risks and complications were more frequently discussed on ENT web pages ( P = .02). ENT sites also had more adverbs ( P = .024) and more uses of passive voice ( P = .006). Additionally, plastic surgery web pages appeared to elicit more of the emotion “disgust” after analysis utilizing IBM Watson Natural Language Understanding ( P < .001). Conclusions: This study highlights the necessity for continued improvement in the readability of webpages designed for patients considering rhinoplasty. Additionally, there were statistically significant differences between decision-making factors presented on ENT-based webpages and plastic-surgery-based webpages. This study may aid surgeons in developing websites that are more accessible, equitable to patients, and contain a more comprehensive discussion regarding key patient decision-making factors and preoperative considerations.
Prevalence and Patterns of Neonatal Birth Trauma in Ethiopia: Systematic Review and Meta-Analysis
Authors: Balis Bikila , Diriba Hunde Aboma , Lami Magarsa
Published: August 2023

Introduction. Neonatal death is still alarming in low-income countries including Ethiopia, accounts 30 death per 1000 alive births. Birth trauma is the second most common contributing factors for the death. Nevertheless, there is no aggregate evidence on the prevalence and patterns of neonatal birth trauma in Ethiopia. Therefore, this study aimed to assess the pooled prevalence and patterns of neonatal birth trauma in Ethiopia. Methods. Searching databases including PubMed, MEDLINE, Popline, SCOPUS, Web of Science, EMBASE, CINHAL (EBSCO), Google, Google Scholar, and lists of references were used to search literatures in Ethiopia. STATA version 14 was used for analysis, and the odds ratios of the outcome variable were determined using the random-effects model. Heterogeneity among the studies was assessed by computing values for I2 and P-values. Also, sensitivity analysis and funnel plot were done to assess the stability of pooled values to outliers and publication bias. Results. A total of 6 studies with a sample size of 3663 were included in this study. The overall prevalence of neonatal birth trauma was 15% (95% CI: 13-16). Subgaleal hemorrhage (39%), cephalohematoma (27%), and caput succedaneum (24%) were the most common neonatal birth trauma in Ethiopia. Meta-analyses and sensitivity analyses showed the stability of the pooled odds ratios, and the funnel plots did not show publication bias. Conclusion. This systematic review and meta-analysis revealed a high prevalence of neonatal birth trauma in Ethiopia. Moreover, most of the neonatal birth injuries were severe and life-threatening that need medical attention to safe the neonates and its life long complications.
MiR-15b-5p and PCSK9 inhibition reduces lipopolysaccharide-induced endothelial dysfunction by targeting SIRT4
Authors: Martino Elisa , D’Onofrio Nunzia , Balestrieri Anna , Mele Luigi , Sardu Celestino , Marfella Raffaele , Campanile Giuseppe , Balestrieri Maria Luisa
Published: August 2023

Background

Endothelial dysfunction and deregulated microRNAs (miRNAs) participate in the development of sepsis and are associated with septic organ failure and death. Here, we explored the role of miR-15b-5p on inflammatory pathways in lipopolysaccharide (LPS)-treated human endothelial cells, HUVEC and TeloHAEC.

Methods

The miR-15b-5p levels were evaluated in LPS-stimulated HUVEC and TeloHAEC cells by quantitative real-time PCR (qRT–PCR). Functional experiments using cell counting kit-8 (CCK-8), transfection with antagomir, and enzyme-linked immunosorbent assays (ELISA) were conducted, along with investigation of pyroptosis, apoptosis, autophagy, and mitochondrial reactive oxygen species (ROS) by cytofluorometric analysis and verified by fluorescence microscopy. Sirtuin 4 (SIRT4) levels were detected by ELISA and immunoblotting, while proprotein convertase subtilisin-kexin type 9 (PCSK9) expression was determined by flow cytometry (FACS) and immunofluorescence analyses. Dual-luciferase reporter evaluation was performed to confirm the miR-15b-5p–SIRT4 interaction.

Results

The results showed a correlation among miR-15b-5p, PCSK9, and SIRT4 levels in septic HUVEC and TeloHAEC. Inhibition of miR-15b-5p upregulated SIRT4 content, alleviated sepsis-related inflammatory pathways, attenuated mitochondrial stress, and prevented apoptosis, pyroptosis, and autophagic mechanisms. Finally, a PCSK9 inhibitor (i-PCSK9) was used to analyze the involvement of PCSK9 in septic endothelial injury. i-PCSK9 treatment increased SIRT4 protein levels, opposed the septic inflammatory cascade leading to pyroptosis and autophagy, and strengthened the protective role of miR-15b-5p inhibition. Increased luciferase signal validated the miR-15b-5p–SIRT4 binding.

Conclusions

Our in vitro findings suggested the miR-15b-5p–SIRT4 axis as a suitable target for LPS-induced inflammatory pathways occurring in sepsis, and provide additional knowledge on the beneficial effect of i-PCSK9 in preventing vascular damage by targeting SIRT4.

Graphical Abstract
Investigating the potential of growing crops hydroponically utilizing feed and draw solutions from fertilizer drawn forward osmosis
Authors: Bassiouny Mohamed , Abdel Maksoud Yasmine , Kimera Fahad , Bahader Khaled , Sewilam Hani
Published: August 2023

This study investigated the potential of utilizing both the draw and feed solutions resulting from fertilizer drawn forward osmosis for hydroponic crop cultivation. Synthetic brackish groundwater of 2500 ppm was used as the feed solution, whereas commercial hydroponic nutrients, sourced from a local supplier, were utilized as a draw solution. This study also investigated the potential of integrating nanofiltration with forward osmosis, but supplementing the water necessary for further dilution of draw solutions through nanofiltration. Two crops were selected, i.e., cherry tomatoes and spinach grown at different water salinities, for their economic values. The cherry tomatoes were grown in Deep Water Culture hydroponic systems, while the spinach was grown in Nutrient Film Technique systems. If this application is deemed feasible, it allows for providing a method to grow two different crops in areas associated with non-arable land and brackish groundwater. During desalination, it was observed that there were two groups of flux readings, the first with an average flux of 7 to 9 l/m2/h, and the other with an average flux of 4 to 6 l/m2/h. This was due to using the same draw solution twice; once to concentrate the feed solution to 5000 ppm, and then once more to concentrate the feed solution to 3500 ppm. It was found that while the 3500 ppm cherry tomatoes tables had the highest yield and highest number of tomatoes throughout the plants lifetime, tomatoes from freshwater tables on average weighed more by about 19%, while, on average, 5000 ppm tomatoes weighed less than 3500 ppm tomatoes by 10%. The results of the spinach demonstrated that while both control and experiment groups yielded similar number of leaves, the average yield per plant for the experiment group was higher than the control group (by 25%).
Stepwise mini-incision microdissection testicular sperm extraction in NOA patients with a history of cryptorchidism: a case–control study
Authors: Xu Shuai , Huang Yuhua , Yao Chencheng , Li Peng , Zhi Erlei , Chen Wei , Deng Cunzhong , Zhao Fujun , Li Zheng , Tian Ruhui
Published: August 2023

Background

Although the orchiopexy is recommended for cryptorchidism to preserve male fertility, non-obstructive azoospermia (NOA) may occur in adulthood. Fortunately, a great many of azoospermic men may obtain sperm by microdissection testicular sperm extraction (mTESE). Due to the potential injuries caused by testicular diagnostic biopsy and vascular damage at the time of orchidopexy, minimal invasiveness is particularly important during mTESE, aims to reduce the surgical damage and avoids secondary testicular failure. This comparative study aims to investigate the efficacy of stepwise mini-incision mTESE technique by comparison with standard mTESE in the treatment of NOA patients with a history of cryptorchidism.

Results

A total of 73 mTESE procedures were divided into two groups: Group 1 included 37 cases performed by stepwise mini-incision mTESE, while Group 2 included 36 cases with standard mTESE. The overall sperm retrieval rate (SRR) in the two groups was 68.5% (50/73), with no significant difference in SRR between Group 1 (78.4%, 29/37) and Group 2 (58.3%, 21/36) (P = 0.1). In addition, 46.0% of the patients (17/37) obtained sperm in the first mini-incision step in Group 1, which was also equal to an overall SRR in Group 2 (58.3%, 21/36) (P = 0.3). The operation time in Group 1 (72.6 ± 33.9 min) was significantly shorter than that in Group 2 (90.4 ± 36.4 min) (P = 0.04). Patients with an orchidopexy age no more than 10 years old had a higher SRR (79.5%, 31/39) than others (55.9%, 19/34) (P = 0.03). There were no postoperative complications including wound infection, scrotal hematoma, persistent pain, and testicular atrophy during a follow-up period of at least 6 months.

Conclusions

In conclusion, our study suggests that the stepwise mini-incision mTESE could be a promising approach for sperm retrieval in NOA men with a history of cryptorchidism. While the technique may potentially reduce operation time and surgical invasiveness, further research is needed to validate these findings on a larger scale. The results also suggest that age at orchidopexy may affect SRR and have important implications for the management of cryptorchidism.
ARCA: the interactive database for arbovirus reported cases in the Americas
Authors: Meneses Maria V. , Riva Alberto , Salemi Marco , Mavian Carla
Published: August 2023

Background

Accurate case report data are essential to understand arbovirus dynamics, including spread and evolution of arboviruses such as Zika, dengue and chikungunya viruses. Giving the multi-country nature of arbovirus epidemics in the Americas, these data are not often accessible or are reported at different time scales (weekly, monthly) from different sources.

Results

We developed a publicly available and user-friendly database for arboviral case data in the Americas: ARCA. ARCA is a relational database that is hosted on the ARCA website. Users can interact with the database through the website by submitting queries through the website, which generates displays results and allows users to download these results in different, convenient file formats. Users can choose to view arboviral case data through a table which containscontaining the number of cases for a particular week, a plot, or through a map.

Conclusion

Our ARCA database is a useful tool for arboviral epidemiology research allowing for complex queries, data visualization, integration, and formatting.
Extant thrips diverged in the early tertiary period
Authors: Wu Chunying , Zhang Hongrui
Published: August 2023

Thysanoptera, commonly known as thrips, are diverse tiny insects whose earliest fossil record dates back to the Triassic period. Since there are few studies on the divergence time of taxa under Thysanoptera, this study used 13 mitochondrial coding protein genes to reconstruct the phylogenetic tree with divergence time of 26 species of this order and show a reliable phylogenetic relationship of thrips species. The time tree of this study shows that most extant thrips diverged in the early Tertiary period, while the fossil records also support that most extinct thrips appeared in this period. This study expands our understanding of the evolution of thrips and provides a feasible way of using multiple mitochondrial genes to establish robust phylogenetic relationships and explore divergence time between species.
Implementation of the trial emulation approach in medical research: a scoping review
Authors: Scola Giulio , Chis Ster Anca , Bean Daniel , Pareek Nilesh , Emsley Richard , Landau Sabine
Published: August 2023

Background

When conducting randomised controlled trials is impractical, an alternative is to carry out an observational study. However, making valid causal inferences from observational data is challenging because of the risk of several statistical biases. In 2016 Hernán and Robins put forward the ‘target trial framework’ as a guide to best design and analyse observational studies whilst preventing the most common biases. This framework consists of (1) clearly defining a causal question about an intervention, (2) specifying the protocol of the hypothetical trial, and (3) explaining how the observational data will be used to emulate it.

Methods

The aim of this scoping review was to identify and review all explicit attempts of trial emulation studies across all medical fields. Embase, Medline and Web of Science were searched for trial emulation studies published in English from database inception to February 25, 2021. The following information was extracted from studies that were deemed eligible for review: the subject area, the type of observational data that they leveraged, and the statistical methods they used to address the following biases: (A) confounding bias, (B) immortal time bias, and (C) selection bias.

Results

The search resulted in 617 studies, 38 of which we deemed eligible for review. Of those 38 studies, most focused on cardiology, infectious diseases or oncology and the majority used electronic health records/electronic medical records data and cohort studies data. Different statistical methods were used to address confounding at baseline and selection bias, predominantly conditioning on the confounders (N = 18/49, 37%) and inverse probability of censoring weighting (N = 7/20, 35%) respectively. Different approaches were used to address immortal time bias, assigning individuals to treatment strategies at start of follow-up based on their data available at that specific time (N = 21, 55%), using the sequential trial emulations approach (N = 11, 29%) or the cloning approach (N = 6, 16%).

Conclusion

Different methods can be leveraged to address (A) confounding bias, (B) immortal time bias, and (C) selection bias. When working with observational data, and if possible, the ‘target trial’ framework should be used as it provides a structured conceptual approach to observational research.
Perspectives on the Doctor of Public Health (DrPH) education among students and alumni in the United States: a cross-sectional national online survey
Authors: Park Chulwoo , Delgado Cindy , Irfan Ans
Published: August 2023

Background

This study explored the current and desired identity of the DrPH degree, focusing on whether the competencies set by the Council on Education for Public Health (CEPH) adequately prepare DrPH graduates for effective public health practice. Additionally, the study investigated the necessity of standardization in DrPH training, referring to a consensus-driven approach that equips future public health practitioners with practical skillsets applicable in real-world scenarios.

Methods

A national cross-sectional online survey titled “National DrPH leaders & practitioners needs assessment” was conducted from November 2020 to February 2021. The survey was based on a self-report by DrPH students and DrPH professionals, consisting of the following two main components: (1) how their DrPH training aligns with CEPH competencies and (2) how they perceive the identity of the DrPH degree. Convenience sampling was used to collect the data, which may have limited representation for all DrPH institutions in the United States.

Results

A total of 222 participants (140 current DrPH students and 82 alumni) completed the survey. The mean of the 10-point Likert scale for the degree to which the DrPH training aligns with 26 CEPH competencies (1: not at all – 10: absolutely) ranged from 6.3 (SD: 2.78) to 7.96 (SD: 2.16). The majority of participants (191/222, 86.04%) were satisfied with the knowledge and skills reflected in their training based on the CEPH competencies. However, more than half of the participants (117/222, 52.70%) sought additional professional development/training outside their institutions. DrPH leaders and practitioners faced barriers where the value of their work might not be fully recognized and endorsed. Participants indicated that the DrPH education should be further distinguished from the PhD education.

Conclusions

The DrPH degree holds significant value within the academic sphere of public health practice in the United States. However, its distinction from PhD programs poses a challenge for employers and organizations in the field, requiring attention from higher education programs. By solidifying the DrPH’s identity, graduates can effectively address diverse public health issues and contribute to creating a safe and healthy environment, including addressing the challenges posed by the COVID-19 pandemic.
Can ultrasound-guided radiofrequency ablation of genicular nerves of the knee, be performed without locating corresponding arterial pulsations—a cadaveric study
Authors: Kolakkanni Chinchu , Gonnade Nitesh Manohar , Gaur Ravi , Nayyar Ashish Kumar , Ghuleliya Rambeer , TK Abins
Published: August 2023

Introduction

Given the rising prevalence of knee osteoarthritis, radiofrequency ablation of genicular nerves (RFA) has emerged as a promising treatment option for knee pain. The knee has an extremely complex and variable innervation with nearly 13 genicular nerves described. The frequently ablated genicular nerves are the superomedial (SMGN), the superolateral (SLGN), and the inferomedial (IMGN) genicular nerves. Conventionally, under ultrasound guidance, these nerves are ablated near the corresponding arterial pulsations, but due to the rich vascular anastomosis around the knee joint, identifying the arteries corresponding to these constant genicular nerves can be tedious unless guided by some bony landmarks. In this study, we have evaluated whether it is possible to accurately target these three genicular nerves by just locating bony landmarks under ultrasound in human cadaveric knee specimens.

Methods

Fifteen formalin-fixed cadaveric knee specimens were studied. SMGN was targeted 1 cm anterior to the adductor tubercle in the axial view. For SLGN, in the coronal view, the junction of the lateral femoral condyle and shaft was identified, and at the same level in the axial view, the crest between the lateral and posterior femoral cortex was targeted. For IMGN in the coronal view, the midpoint between the most prominent part of the medial tibial condyle and the insertion of the deep fibers of the medial collateral ligament was marked. The medial end of the medial tibial cortex was then targeted at the same level in the axial view. The needle was inserted from anterior to posterior, with an in-plane approach for all nerves. Eosin, 2% W/V, in 0.1 ml was injected. Microdissection was done while keeping the needle in situ. Staining of the nerve was considered a positive outcome, and the percentage was calculated. The nerve-to-needle distance was measured, and the mean with an interquartile range was calculated.

Result

The accuracies of ultrasound-guided bony landmarks of SMGN, SLGN, and IMGN were 100% in terms of staining, with average nerve-to-needle distances of 1.67, 3.2, and 1.8 mm respectively.

Conclusion

It is with 100% accuracy, that we can perform RFA of SMGN, SLGN, and IMGN under ultrasound guidance, by locating the aforementioned bony landmarks.
Prevalence of different stages of periodontal diseases among a sample of young adult obese Egyptian patients: a hospital based Cross-sectional study over 1 year
Authors: Abbas Yasmine , Elsaadany Basma , Ghallab Noha
Published: August 2023

Background

This cross-sectional study aimed to determine the prevalence of different stages of periodontal diseases based on the recent classification in a sample of young adult obese Egyptian dental outpatients.

Methods

This study included 314 patients seeking dental treatment at the Diagnostic Center of the Dental hospital, Faculty of Dentistry, Cairo University. Validated oral health questionnaire for adults regarding their age, gender, level of education and oral health routines as well as oral health impact profile questionnaire for chronic periodontitis (OHIP-CP) were filled by all patients. Obesity parameters were also assessed through person’s weight in kilograms, height in centimeters and waist circumference to determine the obesity stage. Diagnosis was made based on measurements of clinical periodontal parameters including a full mouth plaque index (PI), bleeding on probing (BoP), pocket depth (PD), clinical attachment level (CAL) and gingival recession depth (RD). Radiographic examination was performed using periapical radiographs. Ordinal logistic regression analysis was used to determine significant predictors of periodontal diseases and discriminant analysis was performed to predict periodontal disease classification. 

Results

The age range in the study sample was 19–39 years old. The prevalence of different stages of periodontal diseases was 100%. Gingivitis was the most prevalent periodontal disease (63.7%) followed by Periodontitis Stage III (22.6%) then Stage II (11.1%). Stage I showed the least prevalence (2.5%). An increase in BMI was statistically associated with an increase in PD, CAL, RD, PI and vice versa (P-value < 0.05). The total OHIP-CP was 15.99 ± 3.06 for all participants.

Conclusions

There was a statistically significant association between periodontal diseases and obesity in young adults, as well as a statistically significant direct correlation between BMI and periodontal parameters. Self-assessment of oral health and obesity were significant predictors of periodontal disease.

Clinical trial Registration ID

NCT04618068.
Protection of gender health and fight against gender violence during the COVID-19 pandemic: the experience of our street clinic in a disadvantaged suburb of Rome Metropolitan City
Authors: Urbano Suleika , Gobbi Elisabetta , Florio Valeria , Rughetti Aurelia , Ercoli Lucia
Published: August 2023

Object

In this study, we evaluated health, social inequalities and risk to gender violence of women living in a disadvantaged degraded suburb of Rome Metropolitan City, during COVID-19 pandemic.

Methods

 The study included 779 women referring to primary care services of Medicina Solidale Institute for gynecological/breast examinations (209), medical and support aid for the children (383) and COVID-19 test execution (187).

Results

 The data show that most women (68%) were unemployed or had an irregular job. The request of support varied depending on the ethnicity: while healthcare support was requested mostly by African female community, the COVID-19 test, mandatory for public transportation and work, was a need of the east-european community. Both these communities referred to Medical Solidale primary care service for the healthcare and food/clothing support for their children.

It is interesting to note that the requests from the Italian women community was elevated in terms of personal healthcare, support for the children and COVID-19 test execution.

The access to the national health system (NHS) resulted a complex administrative procedure despite the original social-ethnic communities. The vast majority of women lacked awareness of their crucial role for supporting the family entity, while inadequacy was commonly reported.

Conclusions

 This study confirms a critical condition for women living in disadvantaged neighborhoods, whose vulnerability is further worsened by the limited access to primary care assistance with serious consequences for health and quality of life. Prevention and treatment, especially for the most vulnerable subjects, should be a priority for the public health system.
Mutation in yl-HOG1 represses the filament-to-yeast transition in the dimorphic yeast Yarrowia lipolytica
Authors: Rzechonek Dorota A. , Szczepańczyk Mateusz , Mirończuk Aleksandra M.
Published: August 2023

Background

Yarrowia lipolytica is a dimorphic fungus, which switches from yeast to filament form in response to environmental conditions. For industrial purposes it is important to lock cells in the yeast or filamentous form depending on the fermentation process. yl-Hog1 kinase is a key component of the HOG signaling pathway, responsible for activating the osmotic stress response. Additionally, deletion of yl-Hog1 leads to increased filamentation in Yarrowia lipolytica, but causes significant sensitivity to osmotic stress induced by a high concentration of a carbon source.

Results

In this study, we tested the effect of point mutations on the function of yl-Hog1 protein kinase. The targets of modification were the phosphorylation sites (T171A-Y173A) and the active center (K49R). Introduction of the variant HOG1-49 into the hog1∆ strain partially improved growth under osmotic stress, but did not recover the yeast-like shape of the cells. The HOG1-171/173 variant was not functional, and its introduction further weakened the growth of hog1∆ strains in hyperosmotic conditions. To verify a genetic modification in filament form, we developed a new system based on green fluorescent protein (GFP) for easier screening of proper mutants.

Conclusions

These results provide new insights into the functions of yl-Hog1 protein in dimorphic transition and constitute a good starting point for further genetic modification of Y. lipolytica in filament form.
MicroRNAs as the pivotal regulators of cisplatin resistance in head and neck cancers
Authors: Tolue Ghasaban Faezeh , Maharati Amirhosein , Zangouei Amir Sadra , Zangooie Alireza , Moghbeli Meysam
Published: August 2023

Although, there is a high rate of good prognosis in early stage head and neck tumors, about half of these tumors are detected in advanced stages with poor prognosis. A combination of chemotherapy, radiotherapy, and surgery is the treatment option in head and neck cancer (HNC) patients. Although, cisplatin (CDDP) as the first-line drug has a significant role in the treatment of HNC patients, CDDP resistance can be observed in a large number of these patients. Therefore, identification of the molecular mechanisms involved in CDDP resistance can help to reduce the side effects and also provides a better therapeutic management. MicroRNAs (miRNAs) as the post-transcriptional regulators play an important role in drug resistance. Therefore, in the present review we investigated the role of miRNAs in CDDP response of head and neck tumors. It has been reported that the miRNAs exerted their roles in CDDP response by regulation of signaling pathways such as WNT, NOTCH, PI3K/AKT, TGF-β, and NF-kB as well as apoptosis, autophagy, and EMT process. The present review paves the way to suggest a non-invasive miRNA based panel marker for the prediction of CDDP response among HNC patients. Therefore, such diagnostic miRNA based panel marker reduces the CDDP side effects and improves the clinical outcomes of these patients following an efficient therapeutic management.
Both reduced ovarian reserve and severe semen alterations are overrepresented in couples seeking assisted reproductive technology treatment for the first time: a cross-sectional study
Authors: Tarín Juan J. , Pascual Eva , García-Pérez Miguel A. , Monllor-Tormos Aitana , Cano Antonio
Published: August 2023

Background

Once a mate choice decision has been made, couples that fail to reach a live birth in natural and/or intrauterine insemination (IUI) cycles will likely visit fertility clinics seeking assisted reproductive technology (ART) treatment. During the more or less prolonged period of infertility experienced, those couples with mild/moderate reproductive anomalies would have advantage over couples displaying more severe reproductive alterations in achieving a natural or IUI conception. Thus, we can expect to find a progressive increase in the proportion of couples with more severe reproductive anomalies as duration of infertility rises. In this study, we aim to ascertain whether there is an association between male and female infertility diagnoses and duration of infertility in couples seeking ART treatment for the first time.

Methods

A cross-sectional analysis of 1383 infertile couples that sought ART treatment for the first time. Forward-stepwise binary logistic regression analyses were applied to calculate exponentiated regression coefficients.

Results

Men suffering from any combination of oligo-, astheno-, and teratozoospermia (ACOAT) exhibited higher odds of having a duration of infertility > 2 years compared with non-ACOAT men [odds ratio (95% confidence interval): 1.340 (1.030–1.744)]. Women from ACOAT couples displaying a duration of infertility > 2 years presented shorter menstrual cycles (P ≤ 0.047) and lower antral follicular count (AFC) values (P ≤ 0.008) and serum anti-Müllerian hormone (AMH) levels (P ≤ 0.007) than women from non-ACOAT couples exhibiting > 2 years of infertility. Likewise, AFC values (P ≤ 0.013) and serum AMH levels (P ≤ 0.001) were decreased when compared with women from ACOAT couples displaying ≤ 2 years of infertility. A relative low but significant percentage of ACOAT couples displaying > 2 years of infertility stood out for their smoking habits.

Conclusions

Couples consisting of ACOAT men and women with a relative low ovarian reserve are overrepresented in couples seeking ART treatment for the first time after experiencing > 2 years of infertility. This outcome leads us to develop a general hypothesis proposing that the origin of couple’s infertility is a consequence of a process of positive assortative mating shaped by sexual selection forces.
A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs
Authors: Haach Vanessa , Bastos Ana Paula Almeida , Gava Danielle , da Fonseca Francisco Noé , Morés Marcos Antônio Zanella , Coldebella Arlei , Franco Ana Cláudia , Schaefer Rejane
Published: August 2023

Background

Influenza A virus (IAV) is endemic in pigs globally and co-circulation of genetically and antigenically diverse virus lineages of subtypes H1N1, H1N2 and H3N2 is a challenge for the development of effective vaccines. Virosomes are virus-like particles that mimic virus infection and have proven to be a successful vaccine platform against several animal and human viruses.

Methods

This study evaluated the immunogenicity of a virosome-based influenza vaccine containing the surface glycoproteins of H1N1 pandemic, H1N2 and H3N2 in pigs.

Results

A robust humoral and cellular immune response was induced against the three IAV subtypes in pigs after two vaccine doses. The influenza virosome vaccine elicited hemagglutinin-specific antibodies and virus-neutralizing activity. Furthermore, it induced a significant maturation of macrophages, and proliferation of B lymphocytes, effector and central memory CD4+ and CD8+ T cells, and CD8+ T lymphocytes producing interferon-γ. Also, the vaccine demonstrated potential to confer long-lasting immunity until the market age of pigs and proved to be safe and non-cytotoxic to pigs.

Conclusions

This virosome platform allows flexibility to adjust the vaccine content to reflect the diversity of circulating IAVs in swine in Brazil. The vaccination of pigs may reduce the impact of the disease on swine production and the risk of swine-to-human transmission.
Feature enhancement guided network for yield estimation of high-density jujube
Authors: Cheng Fengna , Wei Juntao , Jiang Shengqin , Chen Qing , Ru Yu , Zhou Hongping
Published: August 2023

Background

Automatic and precise jujube yield prediction is important for the management of orchards and the allocation of resources. Traditional yield prediction techniques are based on object detection, which predicts a box to achieve target statistics, but are often used in sparse target settings. Those techniques, however, are challenging to use in real-world situations with particularly dense jujubes. The box labeling is labor- and time-intensive, and the robustness of the system is adversely impacted by severe occlusions. Therefore, there is an urgent need to develop a robust method for predicting jujube yield based on images. But in addition to the extreme occlusions, it is also challenging due to varying scales, complex backgrounds, and illumination variations.

Results

In this work, we developed a simple and effective feature enhancement guided network for yield estimation of high-density jujube. It has two key designs: Firstly, we proposed a novel label representation method based on uniform distribution, which provides a better characterization of object appearance compared to the Gaussian-kernel-based method. This new method is simpler to implement and has shown greater success. Secondly, we introduced a feature enhancement guided network for jujube counting, comprising three main components: backbone, density regression module, and feature enhancement module. The feature enhancement module plays a crucial role in perceiving the target of interest effectively and guiding the density regression module to make accurate predictions. Notably, our method takes advantage of this module to improve the overall performance of our network. To validate the effectiveness of our method, we conducted experiments on a collected dataset consisting of 692 images containing a total of 40,344 jujubes. The results demonstrate the high accuracy of our method in estimating the number of jujubes, with a mean absolute error (MAE) of 9.62 and a mean squared error (MSE) of 22.47. Importantly, our method outperforms other state-of-the-art methods by a significant margin, highlighting its superiority in jujube yield estimation.

Conclusions

The proposed method provides an efficient image-based technique for predicting the yield of jujubes. The study will advance the application of artificial intelligence for high-density target recognition in agriculture and forestry. By leveraging this technique, we aim to enhance the level of planting automation and optimize resource allocation.
miR-181a-5p targets DDX3X to inhibit the progression of osteoarthritis via NF-ΚB signaling pathway
Authors: Zhao Peng , Ma Guobin , Ma Lintong
Published: August 2023

Osteoarthritis (OA) is the most common age-related joint disease, characterized by chronic inflammation, progressive articular cartilage destruction and subchondral osteosclerosis. More and more evidence showed that microRNAs (miRNAs) play a key role in various diseases, but the specific mechanism of miRNAs in OA is not clear. The purpose of this study was to investigate the expression level and role of miR-181a-5p in OA and its related mechanism. Here we identified the key gene DEAD-box RNA helicase 3X (DDX3X) in the OA dataset by bioinformatics analysis. At the same time, miRNAs targeting DDX3X were screened, and miR-181a-5p was selected as the next research object. Then we used different concentrations of interleukin-1 beta (IL-1β)-induced in vitro model of arthritis, and found that IL-1β can stimulate cells to release nitric oxide. The expression levels of miR-181a-5p and DDX3X in mouse chondrocyte cell line ATDC5 induced by IL-1β at a concentration of 10ug/mL were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). IL-1β induced a decrease in the expression of miR-181a-5p and an increase in the expression of DDX3X in ATDC5 cells. mimic miR-181a-5p or inhibitor miR-181a-5p were transfected into ATDC5 cells, and the levels of inflammatory mediators in the cells were detected by enzyme-linked immunosorbent assay, and the results showed that miR-181a-5p could reduce the release of tumor necrosis factor-α, IL-1β, IL-6 and inducible nitric oxide nitric oxide synthase in a cellular model of arthritis. Luciferase reporter assays confirmed that the miR-181a-5p binding site was in the DDX3X gene 3′-untranslated region (3′-UTR), and DDX3X was negatively regulated by miR-181a-5p. Rescue assays confirmed that miR-181a-5p reduced the expression of DDX3X by targeting the 3′-UTR region of DDX3X, thereby reducing the release of inflammatory factors. Finally, in this paper, western blot was used to detect the mechanism of miR-181a-5p regulating OA. The results showed that interfering with the expression of miR-181a-5p could up-regulate the expression of DDX3X protein, increase the expression of nuclear factor- kappaB (NF-κB) related proteins, and reduce the inflammatory response of OA, thereby increasing the secretion of the matrix proteinases MMP-3 and MMP-13. Taken together, the results of the study suggested that miR-181a-5p may be a promising therapeutic target for the treatment of human OA.