bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing Flow Field Performance of a Small Circulating Water Channel Based on Porous Grid Plate
Authors: Zhang Lu , Shan Xiaobiao , Xie Tao
Published: July 2020

Low-cost and high-efficiency circulating water channels are widely used in the hydrodynamic tests of an underwater device. The current research mainly focuses on obtaining a better velocity uniformity of the test section by optimizing the curve function of the boundary in the contraction section. While, for small underwater device, their hydrodynamic characteristics are sensitive to turbulence. Thus, the circulating water channel, which can obtain the required turbulence characteristics, is urgently needed. A small circulating water channel, which can reduce the turbulence intensity based on a porous grid plate and can be used to test the hydrodynamic characteristics of a small underwater device, is designed. The relationships between porosities and resistance coefficients of a porous grid plate are established. The effects of the honeycomb (porosity and thickness); screen (porosity, number of layers, and spacing); and pumping flow rate on the turbulent characteristics of the test section are studied. The relationships between the parameters and the turbulent characteristics of the test section are established, and the methods to achieve the required flow characteristics of the test section are proposed. Experiments are carried out, and the validity of the obtained results is verified. In this work, the turbulence intensity of the fluid field in the test section can be restrained to 0.0491, which is enough to meet the turbulence requirements for the hydrodynamic test of a small underwater device. This work can provide references for the construction of a hydrodynamic test platform for small underwater devices.
The Precipitation Imaging Package: Assessment of Microphysical and Bulk Characteristics of Snow
Authors: Pettersen Claire , Bliven Larry F. , von Lerber Annakaisa , Wood Norman B. , Kulie Mark S. , Mateling Marian E. , Moisseev Dmitri N. , Munchak S. Joseph , Petersen Walter A. , Wolff David B.
Published: July 2020

Remote-sensing observations are needed to estimate the regional and global impacts of snow. However, to retrieve accurate estimates of snow mass and rate, these observations require augmentation through additional information and assumptions about hydrometeor properties. The Precipitation Imaging Package (PIP) provides information about precipitation characteristics and can be utilized to improve estimates of snowfall rate and accumulation. Here, the goal is to demonstrate the quality and utility of two higher-order PIP-derived products: liquid water equivalent snow rate and an approximation of volume-weighted density called equivalent density. Accuracy of the PIP snow rate and equivalent density is obtained through intercomparison with established retrieval methods and through evaluation with colocated ground-based observations. The results confirm the ability of the PIP-derived products to quantify properties of snow rate and equivalent density, and demonstrate that the PIP produces physically realistic snow characteristics. When compared to the National Weather Service (NWS) snow field measurements of six-hourly accumulation, the PIP-derived accumulations were biased only +2.48% higher. Additionally, this work illustrates fundamentally different microphysical and bulk features of low and high snow-to-liquid ratio events, through assessment of observed particle size distributions, retrieved mass coefficients, and bulk properties. Importantly, this research establishes the role that PIP observations and higher-order products can serve for constraining microphysical assumptions in ground-based and spaceborne remotely sensed snowfall retrievals.
Vanillic Acid Improves Comorbidity of Cancer and Obesity through STAT3 Regulation in High-Fat-Diet-Induced Obese and B16BL6 Melanoma-Injected Mice
Authors: Park Jinbong , Cho Seon Yeon , Kang JongWook , Park Woo Yong , Lee Sujin , Jung Yunu , Kang Min-Woo , Kwak Hyun Jeong , Um Jae-Young
Published: July 2020

Obesity is known to be associated with risk and aggressiveness of cancer. Melanoma, the most lethal type of skin cancer, is also closely related to the prevalence of obesity. In this study, we established a cancer–obesity comorbidity (COC) model to investigate the effects of vanillic acid (VA). After a five-week administration with a high-fat diet (HFD) to induce obesity, subcutaneous allograft of B16BL6 cells were followed, and VA was orally administrated for an additional two weeks. VA-fed mice showed significantly decreased body weight and white adipose tissue (WAT) weight, which were due to increased thermogenesis and AMPK activation in WATs. Growth of cancer was also suppressed. Mechanistic studies revealed increased apoptosis and autophagy markers by VA; however, caspase 3 was not involved. Since signal transducer and activator of transcription 3 (STAT3) is suggested as an important pathway linking obesity and cancer, we further investigated to find out if STAT3 phosphorylation was repressed by VA treatment, and this was again confirmed in a COC cell model of adipocyte conditioned medium-treated B16BL6 melanoma cells. Overall, our results show VA induces STAT3-mediated autophagy to inhibit cancer growth and thermogenesis to ameliorate obesity in COC. Based on these findings, we suggest VA as a candidate therapeutic agent for COC treatment.
AGC2 (Citrin) Deficiency—From Recognition of the Disease till Construction of Therapeutic Procedures
Authors: Saheki Takeyori , Moriyama Mitsuaki , Funahashi Aki , Kuroda Eishi
Published: July 2020

Can you imagine a disease in which intake of an excess amount of sugars or carbohydrates causes hyperammonemia? It is hard to imagine the intake causing hyperammonemia. AGC2 or citrin deficiency shows their symptoms following sugar/carbohydrates intake excess and this disease is now known as a pan-ethnic disease. AGC2 (aspartate glutamate carrier 2) or citrin is a mitochondrial transporter which transports aspartate (Asp) from mitochondria to cytosol in exchange with glutamate (Glu) and H+. Asp is originally supplied from mitochondria to cytosol where it is necessary for synthesis of proteins, nucleotides, and urea. In cytosol, Asp can be synthesized from oxaloacetate and Glu by cytosolic Asp aminotransferase, but oxaloacetate formation is limited by the amount of NAD+. This means an increase in NADH causes suppression of Asp formation in the cytosol. Metabolism of carbohydrates and other substances which produce cytosolic NADH such as alcohol and glycerol suppress oxaloacetate formation. It is forced under citrin deficiency since citrin is a member of malate/Asp shuttle. In this review, we will describe history of identification of the SLC25A13 gene as the causative gene for adult-onset type II citrullinemia (CTLN2), a type of citrin deficiency, pathophysiology of citrin deficiency together with animal models and possible treatments for citrin deficiency newly developing.
Mechanisms Underlying Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia
Authors: Eguchi Motoki , Minami Yosuke , Kuzume Ayumi , Chi SungGi
Published: July 2020

FLT3-ITD and FLT3-TKD mutations were observed in approximately 20 and 10% of acute myeloid leukemia (AML) cases, respectively. FLT3 inhibitors such as midostaurin, gilteritinib and quizartinib show excellent response rates in patients with FLT3-mutated AML, but its duration of response may not be sufficient yet. The majority of cases gain secondary resistance either by on-target and off-target abnormalities. On-target mutations (i.e., FLT3-TKD) such as D835Y keep the TK domain in its active form, abrogating pharmacodynamics of type II FLT3 inhibitors (e.g., midostaurin and quizartinib). Second generation type I inhibitors such as gilteritinib are consistently active against FLT3-TKD as well as FLT3-ITD. However, a “gatekeeper” mutation F691L shows universal resistance to all currently available FLT3 inhibitors. Off-target abnormalities are consisted with a variety of somatic mutations such as NRAS, AXL and PIM1 that bypass or reinforce FLT3 signaling. Off-target mutations can occur just in the primary FLT3-mutated clone or be gained by the evolution of other clones. A small number of cases show primary resistance by an FL-dependent, FGF2-dependent, and stromal CYP3A4-mediated manner. To overcome these mechanisms, the development of novel agents such as covalently-coupling FLT3 inhibitor FF-10101 and the investigation of combination therapy with different class agents are now ongoing. Along with novel agents, gene sequencing may improve clinical approaches by detecting additional targetable mutations and determining individual patterns of clonal evolution.
MicroRNAs in Rectal Cancer: Functional Significance and Promising Therapeutic Value
Authors: Imedio Laura , Cristóbal Ion , Rubio Jaime , Santos Andrea , Rojo Federico , García-Foncillas Jesús
Published: July 2020

It is well-known that microRNAs (miRNAs) are critical mediators of initiation and disease progression in many human cancers. Rectal cancer is a highly prevalent tumor, accounting for around one third of newly diagnosed colorectal cancers. The usefulness of miRNAs as clinical biomarkers predictive of the outcome and response to chemoradiotherapy has been well-reported for rectal cancer. However, the existing literature on their functional and therapeutic impact needs to be put in context to clarify their role in disease pathogenesis. Therfore, this review is focused on the functional relevance of miRNAs as key regulators of signaling pathways in rectal cancer and their potential therapeutic value as novel molecular targets in this disease.
Hippo Signaling Pathway as a Central Mediator of Receptors Tyrosine Kinases (RTKs) in Tumorigenesis
Authors: Azad Taha , Rezaei Reza , Surendran Abera , Singaravelu Ragunath , Boulton Stephen , Dave Jaahnavi , Bell John C. , Ilkow Carolina S.
Published: July 2020

The Hippo pathway plays a critical role in tissue and organ growth under normal physiological conditions, and its dysregulation in malignant growth has made it an attractive target for therapeutic intervention in the fight against cancer. To date, its complex signaling mechanisms have made it difficult to identify strong therapeutic candidates. Hippo signaling is largely carried out by two main activated signaling pathways involving receptor tyrosine kinases (RTKs)—the RTK/RAS/PI3K and the RTK-RAS-MAPK pathways. However, several RTKs have also been shown to regulate this pathway to engage downstream Hippo effectors and ultimately influence cell proliferation. In this text, we attempt to review the diverse RTK signaling pathways that influence Hippo signaling in the context of oncogenesis.
TCGA mRNA Expression Analysis of the Heme Biosynthesis Pathway in Diffusely Infiltrating Gliomas: A Comparison of Typically 5-ALA Fluorescent and Non-Fluorescent Gliomas
Authors: Mischkulnig Mario , Kiesel Barbara , Lötsch Daniela , Roetzer Thomas , Borkovec Martin , Wadiura Lisa I. , Mercea Petra A. , Jaklin Florian J. , Hervey-Jumper Shawn , Roessler Karl , Berger Mitchel S. , Widhalm Georg , Erhart Friedrich
Published: July 2020

5-Aminolevulinic acid (5-ALA) is a fluorescent dye that after metabolization to Protoporphyrin IX (PpIX) by the heme biosynthesis pathway typically leads to visible fluorescence in WHO grade IV but not grade II gliomas. The exact mechanism for high PpIX levels in WHO grade IV gliomas and low PpIX levels in WHO grade II gliomas is not fully clarified. To detect relevant changes in mRNA expression, we performed an in-silico analysis of WHO grade II and IV glioma sequencing datasets provided by The Cancer Genome Atlas (TCGA) to investigate mRNA expression levels of relevant heme biosynthesis genes: Solute Carrier Family 15 Member 1 and 2 (SLC15A1 and SLC15A2), Aminolevulinate-Dehydratase (ALAD), Hydroxymethylbilane-Synthase (HMBS), Uroporphyrinogen-III-Synthase (UROS), Uroporphyrinogen-Decarboxylase (UROD), Coproporphyrinogen-Oxidase (CPOX), Protoporphyrinogen-Oxidase (PPOX), ATP-binding Cassette Subfamily B Member 6 (ABCB6)/G Member 2 (ABCG2) and Ferrochelatase (FECH). Altogether, 258 WHO grade II and 166 WHO grade IV samples were investigated. The mRNA expression levels showed significant differences in 8 of 11 examined genes between WHO grade II and IV gliomas. Significant differences in mRNA expression included increases of HMBS, UROD, FECH and PPOX as well as decreases of SLC15A2, ALAD, UROS and ABCB6 in WHO IV gliomas. Since the majority of changes was found in directions that might actually impair PpIX accumulation in WHO grade IV gliomas, additional studies are needed to analyze the corresponding factors of the heme biosynthesis also on protein level.
Early Change in the Plasma Levels of Circulating Soluble Immune Checkpoint Proteins in Patients with Unresectable Hepatocellular Carcinoma Treated by Lenvatinib or Transcatheter Arterial Chemoembolization
Authors: Odagiri Naoshi , Hai Hoang , Thuy Le Thi Thanh , Dong Minh Phuong , Suoh Maito , Kotani Kohei , Hagihara Atsushi , Uchida-Kobayashi Sawako , Tamori Akihiro , Enomoto Masaru , Kawada Norifumi
Published: July 2020

Immune checkpoint inhibitors, combined with anti-angiogenic agents or locoregional treatments (e.g., transarterial chemoembolization (TACE)), are expected to become standard-of-care for unresectable hepatocellular carcinoma (HCC). We measured the plasma levels of 16 soluble checkpoint proteins using multiplexed fluorescent bead-based immunoassays in patients with HCC who underwent lenvatinib (n = 24) or TACE (n = 22) treatment. In lenvatinib-treated patients, plasma levels of sCD27 (soluble cluster of differentiation 27) decreased (p = 0.040) and levels of sCD40 (p = 0.014) and sTIM-3 (p < 0.001) were increased at Week 1, while levels of sCD27 (p < 0.001) were increased significantly at Weeks 2 through 4. At Week 1 of TACE, in addition to sCD27 (p = 0.028), sCD40 (p < 0.001), and sTIM-3 (soluble T-cell immunoglobulin and mucin domain–3) (p < 0.001), levels of sHVEM (soluble herpesvirus entry mediator) (p = 0.003), sTLR-2 (soluble Toll-like receptor 2) (p = 0.009), sCD80 (p = 0.036), sCTLA-4 (soluble cytotoxic T-lymphocyte antigen 4) (p = 0.005), sGITR (soluble glucocorticoid-induced tumor necrosis factor receptor) (p = 0.030), sGITRL (soluble glucocorticoid-induced TNFR-related ligand) (p = 0.090), and sPD-L1 (soluble programmed death-ligand 1) (p = 0.070) also increased. The fold-changes in soluble checkpoint receptors and their ligands, including sCTLA-4 with sCD80/sCD86 and sPD-1 (soluble programmed cell death domain–1) with sPD-L1 were positively correlated in both the lenvatinib and TACE treatment groups. Our results suggest that there are some limited differences in immunomodulatory effects between anti-angiogenic agents and TACE. Further studies from multicenters may help to identify an effective combination therapy.
Telomeres and Telomerase in the Development of Liver Cancer
Authors: in der Stroth Lena , Tharehalli Umesh , Günes Cagatay , Lechel André
Published: July 2020

Liver cancer is one of the most common cancer types worldwide and the fourth leading cause of cancer-related death. Liver carcinoma is distinguished by a high heterogeneity in pathogenesis, histopathology and biological behavior. Dysregulated signaling pathways and various gene mutations are frequent in hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA), which represent the two most common types of liver tumors. Both tumor types are characterized by telomere shortening and reactivation of telomerase during carcinogenesis. Continuous cell proliferation, e.g., by oncogenic mutations, can cause extensive telomere shortening in the absence of sufficient telomerase activity, leading to dysfunctional telomeres and genome instability by breakage–fusion–bridge cycles, which induce senescence or apoptosis as a tumor suppressor mechanism. Telomerase reactivation is required to stabilize telomere functionality and for tumor cell survival, representing a genetic risk factor for the development of liver cirrhosis and liver carcinoma. Therefore, telomeres and telomerase could be useful targets in hepatocarcinogenesis. Here, we review similarities and differences between HCC and iCCA in telomere biology.
Resectable IIIA-N2 Non-Small-Cell Lung Cancer (NSCLC): In Search for the Proper Treatment
Authors: Brascia Debora , De Iaco Giulia , Schiavone Marcella , Panza Teodora , Signore Francesca , Geronimo Alessandro , Sampietro Doroty , Montrone Michele , Galetta Domenico , Marulli Giuseppe
Published: July 2020

Locally advanced non-small cell lung cancer accounts for one third of non-small cell lung cancer (NSCLC) at the time of initial diagnosis and presents with a wide range of clinical and pathological heterogeneity. To date, the combined multimodality approach involving both local and systemic control is the gold standard for these patients, since occult distant micrometastatic disease should always be suspected. With the rapid increase in treatment options, the need for an interdisciplinary discussion involving oncologists, surgeons, radiation oncologists and radiologists has become essential. Surgery should be recommended to patients with non-bulky, discrete, or single-level N2 involvement and be included in the multimodality treatment. Resectable stage IIIA patients have been the subject of a number of clinical trials and retrospective analysis, discussing the efficiency and survival benefits on patients treated with the available therapeutic approaches. However, most of them have some limitations due to their retrospective nature, lack of exact pretreatment staging, and the involvement of heterogeneous populations leading to the awareness that each patient should undergo a tailored therapy in light of the nature of his tumor, its extension and his performance status.
Anti-Proliferative and Pro-Apoptotic Effects of Short-Term Inhibition of Telomerase In Vivo and in Human Malignant B Cells Xenografted in Zebrafish
Authors: Giunco Silvia , Zangrossi Manuela , Dal Pozzolo Francesca , Celeghin Andrea , Ballin Giovanni , Petrara Maria Raffaella , Amin Aamir , Argenton Francesco , Godinho Ferreira Miguel , De Rossi Anita
Published: July 2020

Besides its canonical role in stabilizing telomeres, telomerase reverse transcriptase (TERT) may promote tumor growth/progression through extra-telomeric functions. Our previous in vitro studies demonstrated that short-term TERT inhibition by BIBR1532 (BIBR), an inhibitor of TERT catalytic activity, negatively impacts cell proliferation and viability via telomeres’ length-independent mechanism. Here we evaluate the anti-proliferative and pro-apoptotic effects of short-term telomerase inhibition in vivo in wild-type (wt) and tert mutant (terthu3430/hu3430; tert−/−) zebrafish embryos, and in malignant human B cells xenografted in casper zebrafish embryos. Short-term Tert inhibition by BIBR in wt embryos reduced cell proliferation, induced an accumulation of cells in S-phase and ultimately led to apoptosis associated with the activation of DNA damage response; all these effects were unrelated to telomere shortening/dysfunction. BIBR treatment showed no effects in tert−/− embryos. Xenografted untreated malignant B cells proliferated in zebrafish embryos, while BIBR pretreated cells constantly decreased and were significantly less than those in the controls from 24 to up to 72 h after xenotransplantation. Additionally, xenografted tumor cells, treated with BIBR prior- or post-transplantation, displayed a significant higher apoptotic rate compared to untreated control cells. In conclusion, our data demonstrate that short-term telomerase inhibition impairs proliferation and viability in vivo and in human malignant B cells xenografted in zebrafish, thus supporting therapeutic applications of TERT inhibitors in human malignancies.
Circulating miR-16-5p, miR-92a-3p, and miR-451a in Plasma from Lung Cancer Patients: Potential Application in Early Detection and a Regulatory Role in Tumorigenesis Pathways
Authors: Reis Patricia P. , Drigo Sandra A. , Carvalho Robson F. , Lopez Lapa Rainer Marco , Felix Tainara F. , Patel Devalben , Cheng Dangxiao , Pintilie Melania , Liu Geoffrey , Tsao Ming-Sound
Published: July 2020

Background: Micro(mi)RNAs, potent gene expression regulators associated with tumorigenesis, are stable, abundant circulating molecules, and detectable in plasma. Thus, miRNAs could potentially be useful in early lung cancer detection. We aimed to identify circulating miRNA signatures in plasma from patients with lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC), and to verify whether miRNAs regulate lung oncogenesis pathways. Methods: RNA isolated from 139 plasma samples (40 LUAD, 38 LUSC; 61 healthy/non-diseased individuals) were divided into discovery (38 patients; 21 controls for expression quantification using an 800-miRNA panel; Nanostring nCounter®) and validation (40 patients; 40 controls; TaqMan® RT-qPCR) cohorts. Elastic net, Maximizing-R-Square Analysis (MARSA), and C-Statistics were applied for miRNA signature identification. Results: When compared to healthy individuals, 580 of 606 deregulated miRNAs in LUAD and 221 of 226 deregulated miRNAs in LUSC had significantly increased levels. Among the 10 most significantly overexpressed miRNAs, 6 were common to patients with LUAD and LUSC. Further analysis identified three signatures composed of 12 miRNAs. Signatures included miRNAs commonly overexpressed in patient plasma. Enriched pathways included target genes modulated by three miRNAs in the C-Statistics signature: miR-16-5p, miR-92a-3p, and miR-451a. Conclusions: The 3-miRNA signature (miR-16-5p, miR-92a-3p, miR-451a) had high specificity (100%) and sensitivity (84%) to predict cancer (LUAD and LUSC). These miRNAs are predicted to modulate genes and pathways with known roles in lung tumorigenesis, including EGFR, K-RAS, and PI3K/AKT signaling, suggesting that the 3-miRNA signature is biologically relevant in adenocarcinoma and squamous cell carcinoma of the lung.
Enzymatic Synthesis of Estolides from Castor Oil
Authors: Arslan Amine , Rancke-Madsen Anders , Brask Jesper
Published: July 2020

Estolides are fatty acid polyesters with applications in both industry and consumer products. Recently, reports have emerged detailing lipase-catalyzed synthesis of estolides from free hydroxy fatty acids. In this paper, we describe a simple alternative enzymatic process, in which castor oil is directly converted to an estolide mixture by Candida antarctica lipase A (CALA) catalyzed transesterification. The reaction mixture is analyzed by NMR to determine the estolide number (EN) and MALDI MS to identify individual components, in addition to titration to determine the acid value (AV). Estolide trimers and tetramers (EN 2–3) were formed over 24 h in a system with 2:1 (v/v) castor oil–water. Further, utilizing different lipase specificities, addition of Thermomyces lanuginosus lipase (TLL), allowed the CALA product mixture to be cleaned up by hydrolyzing attached glycerol. In addition, a three-enzyme process is suggested, in which a simple alcohol is added and Candida antarctica lipase B (CALB) is used to esterify carboxylic acids to lower AV.
Synthesis and Characterization of Novel Phyto-Mediated Catalyst, and Its Application for a Selective Oxidation of (VAL) into Vanillin under Visible Light
Authors: Al-Hunaiti Afnan , Mohaidat Qassem , Bsoul Ibrahim , Mahmood Sami , Taher Deeb , Hussein Tareq
Published: July 2020

Here, we report an efficient and highly selective oxidation of lignin model substrate using phyto-mediated ZnFe2O4 nanoparticle using Boswellia carterii extract. The nanocatalyst with an average size of 8 nm showed excellent photocatalytic activity of the synthesized carbonyl containing products under visible light irradiation. The catalytic activity and selectivity towards oxidation of vanillyl alcohol to vanillin with selectivity up to 99% at conversion over 98% and turn-over frequency values up to 1600 h−1 were obtained in the presence of H2O2 and base. The cubic spinel nano-ZnFe2O4 catalyst was characterized by powder-XRD, FESEM, HR-TEM and Mössbauer analysis. The demonstrated catalyst was robust and stable under the reaction conditions. Furthermore, it was easy to be separated from the reaction mixture and be reused for subsequent reactions up to 5 times without significant reactivity or selectivity loss.
Signals Orchestrating Peripheral Nerve Repair
Authors: Rigoni Michela , Negro Samuele
Published: July 2020

The peripheral nervous system has retained through evolution the capacity to repair and regenerate after assault from a variety of physical, chemical, or biological pathogens. Regeneration relies on the intrinsic abilities of peripheral neurons and on a permissive environment, and it is driven by an intense interplay among neurons, the glia, muscles, the basal lamina, and the immune system. Indeed, extrinsic signals from the milieu of the injury site superimpose on genetic and epigenetic mechanisms to modulate cell intrinsic programs. Here, we will review the main intrinsic and extrinsic mechanisms allowing severed peripheral axons to re-grow, and discuss some alarm mediators and pro-regenerative molecules and pathways involved in the process, highlighting the role of Schwann cells as central hubs coordinating multiple signals. A particular focus will be provided on regeneration at the neuromuscular junction, an ideal model system whose manipulation can contribute to the identification of crucial mediators of nerve re-growth. A brief overview on regeneration at sensory terminals is also included.
Hypoglossal Nerve Stimulator Placement for Pediatric Trisomy 21 Patients with Refractory Obstructive Sleep Apnea: A Case Series
Authors: Karlik Joelle B. , Raol Nikhila , Gilbertson Laura
Published: July 2020

Background: Hypoglossal nerve stimulators (HNS) are an increasingly popular form of upper airway stimulation for obstructive sleep apnea (OSA) in adults who are unable to tolerate positive pressure treatment. However, HNS use is currently limited in the pediatric population. Case presentation: We present a case series detailing the anesthetic management of three pediatric trisomy 21 patients receiving HNS for refractory obstructive sleep apnea. The patients tolerated the procedure well and experienced no complications. The average obstructive apnea–hypopnea index (AHI) change was 87.4% with the HNS. Conclusions: Proper anxiolysis, safe and controlled induction, multimodal analgesia, and minimization of post-operative respiratory compromise are all necessary to ensure anesthetic and surgical success. After a tailored anesthetic regimen, proper device placement and close follow-up, our patients had a marked improvement in obstructive symptoms.
A Methodology for the Assessment of Climate Change Adaptation Options for Cultural Heritage Sites
Authors: Carmichael Bethune , Wilson Greg , Namarnyilk Ivan , Nadji Sean , Cahill Jacqueline , Brockwell Sally , Webb Bob , Bird Deanne , Daly Cathy
Published: July 2020

Cultural sites are particularly important to Indigenous peoples, their identity, cosmology and sociopolitical traditions. The benefits of local control, and a lack of professional resources, necessitate the development of planning tools that support independent Indigenous cultural site adaptation. We devised and tested a methodology for non-heritage professionals to analyse options that address site loss, build site resilience and build local adaptive capacity. Indigenous rangers from Kakadu National Park and the Djelk Indigenous Protected Area, Arnhem Land, Australia, were engaged as fellow researchers via a participatory action research methodology. Rangers rejected coastal defences and relocating sites, instead prioritising routine use of a risk field survey, documentation of vulnerable sites using new digital technologies and widely communicating the climate change vulnerability of sites via a video documentary. Results support the view that rigorous approaches to cultural site adaptation can be employed independently by local Indigenous stakeholders.
Single-Shot Electro-Optic Sampling on the Temporal Structure of Laser Wakefield Accelerated Electrons
Authors: Huang Kai , Kotaki Hideyuki , Mori Michiaki , Hayashi Yukio , Nakanii Nobuhiko , Kando Masaki
Published: July 2020

Particle acceleration driven by a high power Ti: sapphire laser has invoked great interest worldwide because of the ultrahigh acceleration gradient. For the aspect of electron acceleration, electron beams with energies over GeV have been generated using the laser wakefield acceleration mechanism. For the optimization of the electron generation process, real-time electron parameter monitors are necessary. One of the key parameters of a high energy particle beam is the temporal distribution, which is closely related with the timing resolution in a pump-probe application. Here, we introduced the electro-optic sampling method to laser wakefield acceleration. Real-time multibunch structures were observed. Careful calculations on the physical processes of signal generation in an electro-optic crystal were performed. Discussions of the methodology are elaborated in detail.
Dasybranchus geojedoensis sp. nov. (Annelida: Capitellidae), a New Capitellid Species from Southern Korea
Authors: Jeong Man-Ki , Soh Ho Young
Published: July 2020

Dasybranchusgeojedoensis sp. nov., obtained from the soft sublittoral bottom in southern Korea, is described as a new species. This new species of Dasybranchus is easily distinguishable from its congeners by the protruded parapodial lobes in the final few thoracic chaetigers. Dasybranchus geojedoensis sp. nov. closely resembles D. bipartitus (Schmarda, 1861) in number and position of abdominal branchiae but differs in position of genital pores and lateral organs, range of thoracic tessellated chaetigers, and denticulation of the abdominal hooks. The partial mtCOI gene sequences of the new Korean species were significantly distinct from those of undescribed species of Dasybranchus from Australia. In this study, a key for known species of Dasybranchus is also provided.
Comparative Dynamics of Individual Ageing among the Investigative Type of Professionals Living in Russia and Russian Migrants to the EU Countries
Authors: Berezina Tatiana N. , Rybtsova Natalia N. , Rybtsov Stanislav A.
Published: July 2020

The goal of this study was to uncover the influence of professional activity, migration, and gender on dynamics of subjective age and ageing biomarkers. We examined the representatives of investigative types of professions (ITP), 30–75 years old in Russia, (101/62 women), and Russian migrants to the European Union, (101/56 women). ITPs appeared to be ageing slower than statistical standards; men age faster than women; the pre-retirement group (51–65 years old) showed acceleration of relative biological ageing in the Russian sample (women +4.5 years, men +10.7 years) against the EU sample, suggesting a boost of pre-retirement stress in Russia; subjectively, Russian people (51–65 years old) feel close to their chronological age, while EU people perceive themselves far below their calendar age (men—lower by 20.4, women—lower by 10.9 years). The subjective ageing depends on the country of residence, while biological ageing depends on occupation, gender, and negative expectations of retirement.
Innovative Reconfigurable Metasurface 2-D Beam-Steerable Reflector for 5G Wireless Communication
Authors: Rotshild David , Rahamim Efraim , Abramovich Amir
Published: July 2020

A tunable reflector component based on metasurface (MS) with a low profile and reduced mass is offered for indoor and outdoor 5G communication methods to overcome obstacles such as buildings, walls, and turns, and to allow wireless quasi-line of sight path communication at 37 GHz. Integrating varactors with MS unit cells allows tunability and reconfigurability. This approach was presented in many studies, with frequencies of up to K–band. However, today, higher frequencies are used, especially in communication. This work presents the design of a reconfigurable MS reflector, at Ka-band frequencies, based on a new type of resonant unit cell, with uniformed reflection for wide-incident-angular-range, and a simple stimulating DC bias for each MS unit cell, which allows a two–dimensional (2-D) continuous reflection phase manipulation. The unit cell provides a dynamic reflection phase range of over 300° at a wide bandwidth. Simulations of one-dimensional (1-D) and (2-D) at 37 GHz are presented. A steering range of up to ±48° was obtained for azimuth or elevation. A simultaneous independent 2-D beam steering range of up to ±10° in azimuth and up to ± 5° in elevation, allowing obstacles to overcome covering at a practical angular spatial cone of 20° and 10°, is presented.
Alleviation of Cadmium Adverse Effects by Improving Nutrients Uptake in Bitter Gourd through Cadmium Tolerant Rhizobacteria
Authors: Zafar-ul-Hye Muhammad , Naeem Muhammad , Danish Subhan , Fahad Shah , Datta Rahul , Abbas Mazhar , Rahi Ashfaq Ahmad , Brtnicky Martin , Holátko Jiří , Tarar Zahid Hassan , Nasir Muhammad
Published: July 2020

Cadmium is acute toxicity inducing heavy metal that significantly decreases the yield of crops. Due to high water solubility, it reaches the plant tissue and disturbs the uptake of macronutrients. Low uptake of nutrients in the presence of cadmium is a well-documented fact due to its antagonistic relationship with those nutrients, i.e., potassium. Furthermore, cadmium stressed plant produced a higher amount of endogenous stress ethylene, which induced negative effects on yield. However, inoculation of 1-amino cyclopropane-1-carboxylate deaminase (ACCD), producing plant growth promoting rhizobacteria (PGPR), can catabolize this stress ethylene and immobilized heavy metals to mitigate cadmium adverse effects. We conducted a study to examine the influence of ACCD PGPR on nutrients uptake and yield of bitter gourd under cadmium toxicity. Cadmium tolerant PGPRs, i.e., Stenotrophomonas maltophilia and Agrobacterium fabrum were inoculated solely and in combination with recommended nitrogen, phosphorus, and potassium fertilizers (RNPKF) applied under different concentration of soil cadmium (2 and 5 mg kg−1 soil). Results showed that A. fabrum with RNPKF showed significant positive response towards an increase in the number of bitter gourds per plant (34% and 68%), fruit length (19% and 29%), bitter gourd yield (26.5% and 21.1%), N (48% and 56%), and K (72% and 55%) concentration from the control at different concentrations of soil cadmium (2 and 5 mg kg−1 soil), respectively. In conclusion, we suggest that A. fabrum with RNPKF can more efficaciously enhance N, K, and yield of bitter gourd under cadmium toxicity.
Estimation of Yield Loss Due to Deer Browsing in a Short Rotation Coppice Willow Plantation in Northern Japan
Authors: Harayama Hisanori , Han Qingmin , Ishihara Makoto , Kitao Mitsutoshi , Uemura Akira , Sasaki Shozo , Yamada Takeshi , Utsugi Hajime , Maruyama Yutaka
Published: July 2020

Deer browsing is a major factor causing significant declines in yield in short rotation coppice (SRC) willow, but the resultant yield loss is difficult to estimate because it requires extensive investigation, especially when the standard yield is unknown. We investigated a simple method for estimating yield loss due to deer browsing. We enclosed an experimental SRC willow plantation in Hokkaido, northern Japan, planted with 12 clones, with an electric fence; deer browsing did, however, occur in the first summer of the second harvest cycle. We counted the number of sprouting stems and deer-browsed stems per plant and, after three years, the yield of each clone was analyzed using a generalized linear model with the above two parameters for the numbers of stems as explanatory variables. The model explained the yield of 11 out of the 12 clones, and estimated that browsing of a single stem per plant could reduce yield to 80%. Losses due to deer browsing were estimated to be as much as 6.0 oven dry ton ha−1 yr−1. The potential yield in the absence of deer browsing ranged from 2.2 to 7.5 oven dry ton ha−1 yr−1 among clones, and was significantly positively correlated with the estimated yield loss due to deer browsing. Our results suggest that a generalized linear model can be used to estimate the yield loss due to deer browsing from a simple survey, and deer browsing could significantly reduce willow biomass yield from the clones we studied, and thus countermeasures to control deer browsing are therefore necessary if sufficient willow biomass yield is to be produced.
Considerations on the Implications of the Internet of Things in Spanish Universities: The Usefulness Perceived by Professors
Authors: Romero-Rodríguez José-María , Alonso-García Santiago , Marín-Marín José-Antonio , Gómez-García Gerardo
Published: July 2020

Internet of Things (IoT) is an emerging technology in the field of education, which has not yet been consolidated. Acceptance and adoption studies of IoT in higher education are scarce. Accordingly, the purpose of this study was to explore the acceptance of the IoT by university professors for future adoption in higher education. An online survey was implemented based on the unified theory of acceptance and use of technology (UTAUT), in a sample of 587 Spanish university teachers, aged between 21 and 58. The results showed that performance expectancy, facilitating conditions, and attitude toward using technology were influential in behavioral intention to use IoT. While the intention for use was similar between men and women and with respect to age. However, in the different constructs of the UTAUT model, the highest average scores were obtained in men and in teachers over 36 years of age. Finally, the findings and implications of the paper are discussed, showing empirical evidence on the adoption and acceptance of IoT in higher education in the context of Spain, highlighting the need for further research on emerging technologies in a context that is marked by COVID-19.
Scientific Development of Educational Artificial Intelligence in Web of Science
Authors: Moreno-Guerrero Antonio-José , López-Belmonte Jesús , Marín-Marín José-Antonio , Soler-Costa Rebeca
Published: July 2020

The social and technological changes that society is undergoing in this century are having a global influence on important aspects such as the economy, health and education. An example of this is the inclusion of artificial intelligence in the teaching–learning processes. The objective of this study was to analyze the importance and the projection that artificial intelligence has acquired in the scientific literature in the Web of Science categories related to the field of education. For this, scientific mapping of the reported documents was carried out. Different bibliometric indicators were analyzed and a word analysis was carried out. We worked with an analysis unit of 379 publications. The results show that scientific production is irregular from its beginnings in 1956 to the present. The language of greatest development is English. The most significant publication area is Education Educational Research, with conference papers as document types. The underlying organization is the Open University UK. It can be concluded that there is an evolution in artificial intelligence (AI) research in the educational field, focusing in the last years on the performance and influence of AI in the educational processes.
Metabolite Profiling and Chemometric Study for the Discrimination Analyses of Geographic Origin of Perilla (Perilla frutescens) and Sesame (Sesamum indicum) Seeds
Authors: Kim Tae Jin , Park Jeong Gon , Kim Hyun Young , Ha Sun-Hwa , Lee Bumkyu , Park Sang Un , Seo Woo Duck , Kim Jae Kwang
Published: July 2020

Perilla and sesame are traditional sources of edible oils in Asian and African countries. In addition, perilla and sesame seeds are rich sources of health-promoting compounds, such as fatty acids, tocopherols, phytosterols and policosanols. Thus, developing a method to determine the geographic origin of these seeds is important for ensuring authenticity, safety and traceability and to prevent cheating. We aimed to develop a discriminatory predictive model for determining the geographic origin of perilla and sesame seeds using comprehensive metabolite profiling coupled with chemometrics. The orthogonal partial least squares-discriminant analysis models were well established with good validation values (Q2 = 0.761 to 0.799). Perilla and sesame seed samples used in this study showed a clear separation between Korea and China as geographic origins in our predictive models. We found that glycolic acid could be a potential biomarker for perilla seeds and proline and glycine for sesame seeds. Our findings provide a comprehensive quality assessment of perilla and sesame seeds. We believe that our models can be used for regional authentication of perilla and sesame seeds cultivated in diverse geographic regions.
Myogenic Response to Increasing Concentrations of Ammonia Differs between Mammalian, Avian, and Fish Species: Cell Differentiation and Genetic Study
Authors: Miramontes Emily , Kempisty Bartosz , Petitte James , Dasarathy Srinivasan , Kulus Magdalena , Wieczorkiewicz Maria , Mozdziak Paul
Published: July 2020

Ammonia is very toxic to the body and has detrimental effects on many different organ systems. Using cultured myoblast cells, we examined ammonia’s effect on myostatin expression, a negative regulator of skeletal muscle growth, and myotube diameters. The objective of this study was to examine how murine, avian, and fish cells respond to increasing levels of ammonia up to 50 mM. The murine myoblast cell line (C2C12), primary chick, and primary tilapia myoblast cells were cultured and then exposed to 10, 25, and 50 mM ammonium acetate, sodium acetate, and an untreated control for 24 h. High levels of ammonia were detrimental to the C2C12 cells, causing increased Myostatin (MSTN) expression and decreased myotube diameters between 10 and 25 mM (p < 0.002). Ammonia at 10 mM continued the positive myogenic response in the chick, with lower MSTN expression than the C2C12 cells and larger myotube diameters, but the myotube diameter at 50 mM ammonium acetate was significantly smaller than those at 10 and 25 mM (p < 0.001). However, chick myotubes at 50 mM were still significantly larger than the sodium acetate-treated and untreated control (p < 0.001). The tilapia cells showed no significant difference in MSTN expression or myotube diameter in response to increasing the concentrations of ammonia. Overall, these results confirm that increasing concentrations of ammonia are detrimental to mammalian skeletal muscle, while chick cells responded positively at lower levels but began to exhibit a negative response at higher levels, as the tilapia experienced no detrimental effects. The differences in ammonia metabolism strategies between fish, avian, and mammalian species could potentially contribute to the differences between species in response to high levels of ammonia. Understanding how ammonia affects skeletal muscle is important for the treatment of muscle wasting observed in liver failure patients.
The Function of BARD1 in Centrosome Regulation in Cooperation with BRCA1/OLA1/RACK1
Authors: Otsuka Kei , Yoshino Yuki , Qi Huicheng , Chiba Natsuko
Published: July 2020

Breast cancer gene 1 (BRCA1)-associated RING domain protein 1 (BARD1) forms a heterodimer with BRCA1, a tumor suppressor associated with hereditary breast and ovarian cancer. BRCA1/BARD1 functions in multiple cellular processes including DNA repair and centrosome regulation. Centrosomes are the major microtubule-organizing centers in animal cells and are critical for the formation of a bipolar mitotic spindle. BRCA1 and BARD1 localize to the centrosome during the cell cycle, and the BRCA1/BARD1 dimer ubiquitinates centrosomal proteins to regulate centrosome function. We identified Obg-like ATPase 1 (OLA1) and receptor for activated C kinase (RACK1) as BRCA1/BARD1-interating proteins that bind to BARD1 and BRCA1 and localize the centrosomes during the cell cycle. Cancer-derived variants of BRCA1, BARD1, OLA1, and RACK1 failed to interact, and aberrant expression of these proteins caused centrosome amplification due to centriole overduplication only in mammary tissue-derived cells. In S-G2 phase, the number of centrioles was higher in mammary tissue-derived cells than in cells from other tissues, suggesting their involvement in tissue-specific carcinogenesis by BRCA1 and BARD1 germline mutations. We described the function of BARD1 in centrosome regulation in cooperation with BRCA1/OLA1/RACK1, as well as the effect of their dysfunction on carcinogenesis.
Determinants of Copper Resistance in Acidithiobacillus Ferrivorans ACH Isolated from the Chilean Altiplano
Authors: Barahona Sergio , Castro-Severyn Juan , Dorador Cristina , Saavedra Claudia , Remonsellez Francisco
Published: July 2020

The use of microorganisms in mining processes is a technology widely employed around the world. Leaching bacteria are characterized by having resistance mechanisms for several metals found in their acidic environments, some of which have been partially described in the Acidithiobacillus genus (mainly on ferrooxidans species). However, the response to copper has not been studied in the psychrotolerant Acidithiobacillus ferrivorans strains. Therefore, we propose to elucidate the response mechanisms of A. ferrivorans ACH to high copper concentrations (0–800 mM), describing its genetic repertoire and transcriptional regulation. Our results show that A. ferrivorans ACH can grow in up to 400 mM of copper. Moreover, we found the presence of several copper-related makers, belonging to cop and cus systems, as well as rusticyanins and periplasmatic acop protein in the genome. Interestingly, the ACH strain is the only one in which we find three copies of copB and copZ genes. Moreover, transcriptional expression showed an up-regulation response (acop, copZ, cusA, rusA, and rusB) to high copper concentrations. Finally, our results support the important role of these genes in A. ferrivorans copper stress resistance, promoting the use of the ACH strain in industrial leaching under low temperatures, which could decrease the activation times of oxidation processes and the energy costs.
Comprehensive Genomic Analysis Reveals the Prognostic Role of LRRK2 Copy-Number Variations in Human Malignancies
Authors: Lopez Gianluca , Lazzeri Giulia , Rappa Alessandra , Isimbaldi Giuseppe , Cribiù Fulvia Milena , Guerini-Rocco Elena , Ferrero Stefano , Vaira Valentina , Di Fonzo Alessio
Published: July 2020

Genetic alterations of leucine-rich repeat kinase 2 (LRRK2), one of the most important contributors to familial Parkinson’s disease (PD), have been hypothesized to play a role in cancer development due to demographical and preclinical data. Here, we sought to define the prevalence and prognostic significance of LRRK2 somatic mutations across all types of human malignancies by querying the publicly available online genomic database cBioPortal. Ninety-six different studies with 14,041 cases were included in the analysis, and 761/14,041 (5.4%) showed genetic alterations in LRRK2. Among these, 585 (76.9%) were point mutations, indels or fusions, 168 (22.1%) were copy number variations (CNVs), and 8 (1.0%) showed both types of alterations. One case showed the somatic mutation R1441C. A significant difference in terms of overall survival (OS) was noted between cases harboring somatic LRRK2 whole deletions, amplifications, and CNV-unaltered cases (median OS: 20.09, 57.40, and 106.57 months, respectively; p = 0.0008). These results suggest that both LRRK2 amplifications and whole gene deletions could play a role in cancer development, paving the way for future research in terms of potential treatment with LRRK2 small molecule inhibitors for LRRK2-amplified cases.