bluuebunny/crossref_metadata_2025 huggingface dataset:
Utvärdering av övningskonceptet Giron 2022
Authors: Björnqvist Anton , Lindhagen Alva , Jonson Carl-Oscar
Published: December 2023

Introduktion
Mellan den 13:e och 15:e september 2022 anordnades övning Giron 2022 i Kiruna. Ett av målen med övningen var att den skulle kunna visa på ett övningskoncept som med regionala anpassningar kan användas för att öka takten inom utvecklingen av den civila beredskapen i Sverige. För att undersöka eventuella utvecklingsbehov kopplat till övningskonceptet utvärderades konceptet med fokus på övningsplaneringen och -genomförandet.
Metod
Vid utvärderingen användes en konsensusorienterad metod bestående av två workshops och en enkät däremellan. Under den första workshopen ombads de 27 deltagarna anteckna utvecklingsförslag baserat på lärdomar från övningsplaneringen och -genomförandet.
Utifrån deltagarnas anteckningar skapades en enkät med 55 påståenden. Denna enkät skickades till de 49 individer som var inbjudna till den andra workshopen. I enkäten ombads de 37 respondenterna besvara varje påstående på skalan ett till nio, där ett motsvarade instämmer inte alls och nio instämmer fullständigt. Enkätsvaren användes för att beräkna huruvida påståendena nådde konsensus, vilket antingen kunde vara för att behålla eller för att förkasta.
De påståenden som inte nådde konsensus i enkäten omformulerades inför den andra workshopen. Under detta tillfälle diskuterade de 32 deltagarna dessa påståenden, följt av en separat omröstning för varje påstående enligt samma metodik som i enkäten. Svaren från omröstningen användes för att beräkna konsensus likt enkätsvaren.
Resultat
De påståenden som nådde konsensus från enkäten och den andra workshopen delades in i sex teman och presenterades i en konsensusskrift. De sex temana är: övningskonceptet generellt, planering, föreläsningar, syfte och mål, övningsupplägg och utvärdering.
Påståendena visar bland annat att övningskonceptet fortsatt ska användas och utvecklas, att övningars syfte i främsta hand bör vara att höja förmågor snarare än bibehålla förmågor, att det ska läggas ett stort fokus på beslutsfattande i framtida övningar och att tekniska hjälpmedel såsom Rakel, WIS och GIS ska användas under övningar.
Slutsats
För att utveckla övningskonceptet bör övningsorganisatörer som planerar genomföra framtida liknande övningar utifrån övningskonceptet Giron 2022 använda konsensusskriften som riktlinje under övningsplaneringen och -genomförandet.
Training Effectiveness Factors Associated with Laypeople Hemorrhage Control Training
Authors: Friberg Marc , Brodin Wilhelm , Arkestål Lukas , Jonson Carl-Oscar , Prytz Erik
Published: December 2023

Introduction
Hemorrhage control initiated by laypeople, or immediate responders, can be a crucial link for increased survival among victims with hemorrhage. An increased interest in hemorrhage control training for laypeople have resulted in numerous publications in the area, showing that training laypeople in hemorrhage control can be successful (Nichols & Horstman, 2022; Tang et al., 2022). To this date, it is unknown which training factors are associated with successful training outcomes and to what extent. The objective of this study is to review and analyze past and present literature on hemorrhage control training for laypeople, in order to highlight current knowledge gaps, and direct future research.
Method
A systematic literature review was performed by reviewing and analyzing the literature. Five electronic databases, CINAHL, Embase, PubMed, Scopus, and Web of Science, were used in the literature search. Search keywords were chosen among the variations and synonyms to the words: “laypeople” AND “hemorrhage” AND “training” AND “stop the bleed”. To examine where the knowledge gaps exist in terms of training effectiveness, a theoretical model by Cannon-Bowers et al. (1995) was chosen as theoretical lens, and each record was categorized into six training outcome categories: reactions, attitude change, learning, training performance, job performance, and results/organizational effectiveness, according to the content of the reviewed studies.
Preliminary results
In total, 2962 records were initially identified for screening, and 1186 records were kept after duplicate removal and deletion of incomplete records. After title and abstract review, 186 records were sought for retrieval. This will be further reduced into a fewer number of records for full text review after additional screening. By categorizing the records into the six training outcome categories, previous research efforts can be highlighted, as well as it creates an opportunity for future research suggestions.
Conclusion
By equipping the public with the necessary knowledge and tools for hemorrhage control, lives can be saved. By examining the literature on hemorrhage control training effectiveness, suggestions for future research and educational practice can be made.
Population-based incidence of all-cause anaphylaxis and its development over time: a systematic review and meta-analysis
Authors: Pühringer Vanessa , Jilma Bernd , Herkner Harald
Published: December 2023

Introduction

It is extremely difficult to compare studies investigating the frequency of anaphylaxis making it challenging to satisfactorily assess the worldwide incidence rate. Using a systematic review and meta-analysis, this publication aims to determine the current incidence of all-cause anaphylaxis worldwide. Additionally, we investigated whether the incidence of anaphylaxis has changed over time and which factors influence the rates determined by individual studies.

Methods

A literature search was performed in four databases. All articles that reported relevant information on population-based incidence rates of all-cause anaphylaxis were included. The protocol was published on INPLASY, the International Platform of Registered Systematic Review and Meta-analysis Protocols.

Results

The database query and screening process resulted in 46 eligible articles on anaphylaxis. The current incidence worldwide was found to be approximately 46 cases per 100,000 population per year (95% CI 21–103). Evaluating confounding factors showed that studies using allergy clinics and hospitalizations as data source result in comparably low rates. Moreover, children are less prone to develop anaphylaxis compared to the general population. Using a random effects Poisson model we calculated a yearly increase of anaphylaxis incidence by 7.4% (95% CI 7.3–7.6, p < 0.05).

Discussion

This seems to be the first approach to analyze every reported all-cause anaphylaxis incidence rate until 2017 for an at most accurate determination of its epidemiology. Based on these results, future research could investigate the underlying causes for the rising incidence in order find ways to decrease the condition’s frequency.

Systematic Review Registration

inplasy.com, identifier [INPLASY202330047]
Au nanorod assembly for sensitive SERS detection of airway inflammatory factors in sputum
Authors: Yang An-qi , Zheng Wentong , Chen Xiaoyang , Wang Jiayin , Zhou Shuang , Gao Hongzhi
Published: December 2023

In this paper, we demonstrate a surface-enhanced Raman spectroscopy (SERS) biosensor based on the self-assembly of gold nanorods (AuNRs) for the specific detection of airway inflammatory factors in diluted sputum. The AuNR surface was modified with an antibody that was able to specifically recognize an airway inflammatory factor, interleukin-5 (IL-5), so that a end-to-end self-assembly system could be obtained, resulting in an order of magnitude amplification of the Raman signal and greatly improved sensitivity. Meanwhile, the outer layer of the biosensor was coated with silicon dioxide, which improved the stability of the system and facilitated its future applications. When the detected concentration was in the range of 0.1–50 pg/mL, the SERS signal generated by the sensor showed a good linear relationship with the IL-5 concentration. Moreover, it had satisfactory performance in diluted sputum and clinical subjects with asthma, which could achieve sensitive detection of the airway inflammatory factor IL-5. Overall, the developed biosensor based on the SERS effect exhibited the advantages of rapid and sensitive detecting performance, which is suitable for monitoring airway inflammatory factors in sputum.
Preparation and reverse recycling logistics of a new type of nano-filled antibacterial layer packaging film for dairy products
Authors: Li Quan , Zhou Xiaorong , Wu Hong
Published: December 2023

Introduction: Dairy products are loved by people because of their high nutritional value, but they have also become the most ideal breeding places for microorganisms. Some dairy packaging has the problem of lax sealing, resulting in products susceptible to contamination and deterioration. The harmful microorganisms and bacteria contained in them will pose a serious threat to people’s health. Therefore, a good antibacterial protection is very important for dairy products. The purpose of this paper is to study the preparation and reverse recycling logistics of a new type of nano-filled antibacterial layer packaging film for dairy products.

Methods: A new type of nano-filled antibacterial layer packaging film is prepared by extrusion casting method, and its mechanical properties and antibacterial properties are analyzed.

Results: The experimental results in this article show that the prepared new nano-filled antibacterial layer packaging film has lower light transmittance and water vapor transmission rate, and has obvious antibacterial properties against Staphylococcus aureus and Escherichia coli, and has good barrier properties.

Discussion: The antibacterial rate of the bacteria in the petri dish is as high as 99.97% after being placed for 120 days, and the antibacterial performance can be enhanced by the ratio of glycerol and starch content, and the new nano-filled antibacterial film prepared is degradable Sex, can be better recycled.
Metagenomic identification of pathogens and antimicrobial-resistant genes in bacterial positive blood cultures by nanopore sequencing
Authors: Liu Yahui , Xu Yumei , Xu Xinyu , Chen Xianghui , Chen Hongli , Zhang Jingjing , Ma Jiayu , Zhang Wenrui , Zhang Rong , Chen Jie
Published: December 2023

Nanopore sequencing workflows have attracted increasing attention owing to their fast, real-time, and convenient portability. Positive blood culture samples were collected from patients with bacterial bloodstream infection and tested by nanopore sequencing. This study compared the sequencing results for pathogen taxonomic profiling and antimicrobial resistance genes to those of species identification and phenotypic drug susceptibility using traditional microbiology testing. A total of 37 bacterial positive blood culture results of strain genotyping by nanopore sequencing were consistent with those of mass spectrometry. Among them, one mixed infection of bacteria and fungi was identified using nanopore sequencing and confirmatory quantitative polymerase chain reaction. The amount of sequencing data was 21.89 ± 8.46 MB for species identification, and 1.0 MB microbial strain data enabled accurate determination. Data volumes greater than or equal to 94.6 MB nearly covered all the antimicrobial resistance genes of the bacteria in our study. In addition, the results of the antimicrobial resistance genes were compared with those of phenotypic drug susceptibility testing for Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus. Therefore, the nanopore sequencing platform for rapid identification of causing pathogens and relevant antimicrobial resistance genes complementary to conventional blood culture outcomes may optimize antimicrobial stewardship management for patients with bacterial bloodstream infection.
Type 2 diabetes mellitus and the risk of male infertility: a Mendelian randomization study
Authors: Zhu Xiao-Bin , Niu Zhi-Hong , Fan Wei-Min , Sheng Chang-Sheng , Chen Qian
Published: December 2023

Objective

To assess the causal effect of type 2 diabetes mellitus (T2DM) on male infertility (MI) and erectile dysfunction (ED) by Mendelian randomization (MR) analysis.

Methods

Data for T2DM, MI, and ED were obtained from genome-wide association studies (GWAS) involving 298, 957, 73, 479, and 223, 805 Europeans, respectively. We performed univariate MR analysis using MR Egger, Weighted median (WM) and Inverse variance weighted (IVW) methods to assess causal effects among the three. Through the Genotype Tissue Expression (GTEx) database, single-nucleotide polymorphisms (SNPs) that affect the expression levels of T2DM-related genes were located using expression quantitative trait loci (eQTL).

Results

MR analysis showed a significant causal relationship between T2DM and ED (WM, OR: 1.180, 95%CI: 1.010-1.378, P = 0.037; IVW, OR: 1.190, 95%CI: 1.084-1.300, P < 0.001). There is also a significant causal relationship between T2DM and MI (MR Egger, OR: 0.549, 95%CI: 0.317-0.952, P = 0.037; WM, OR: 0.593, 95%CI: 0.400, P = 0.010; IVW, OR: 0.767, 95%CI: 0.600-0.980, P = 0.034). ED may not cause MI (P > 0.05). We also found that rs6585827 corresponding to the PLEKHA1 gene associated with T2DM is an eQTL variant affecting the expression of this gene.

Conclusion

T2DM has a direct causal effect on ED and MI. The level of PLEKHA1 expression suppressed by rs6585827 is potentially associated with a lower risk of T2DM.
Causal effects of non-alcoholic fatty liver disease on osteoporosis: a Mendelian randomization study
Authors: Zhou Yue , Ni Yunzhi , Wang Zhihong , Prud’homme Gerald J. , Wang Qinghua
Published: December 2023

Background

Osteoporosis (OP) is a systemic skeletal disease characterized by compromised bone strength leading to an increased risk of fracture. There is an ongoing debate on whether non-alcoholic fatty liver disease (NAFLD) is an active contributor or an innocent bystander in the pathogenesis of OP. The aim of this study was to assess the causal association between NAFLD and OP.

Methods

We performed two‐sample Mendelian randomization (MR) analyses to investigate the causal association between genetically predicted NAFLD [i.e., imaging‐based liver fat content (LFC), chronically elevated serum alanine aminotransferase (cALT) and biopsy-confirmed NAFLD] and risk of OP. The inverse variant weighted method was performed as main analysis to obtain the causal estimates.

Results

Imaging-based LFC and biopsy-confirmed NAFLD demonstrated a suggestive causal association with OP ([odds ratio (OR): 1.003, 95% CI: 1.001-1.004, P < 0.001; OR: 1.001, 95% CI: 1.000-1.002, P = 0.031]). The association between cALT and OP showed a similar direction, but was not statistically significant (OR: 1.001, 95% CI: 1.000-1.002, P = 0.079). Repeated analyses after exclusion of genes associated with confounding factors showed consistent results. Sensitivity analysis indicated low heterogeneity, high reliability and low pleiotropy of the causal estimates.

Conclusion

The two‐sample MR analyses suggest a causal association between genetically predicted NAFLD and OP.
Interorgan communication with the liver: novel mechanisms and therapeutic targets
Authors: Zhao Jiulu , Zhang Xi , Li Yuan , Yu Jizhang , Chen Zhang , Niu Yuqing , Ran Shuan , Wang Song , Ye Weicong , Luo Zilong , Li Xiaohan , Hao Yanglin , Zong Junjie , Xia Chengkun , Xia Jiahong , Wu Jie
Published: December 2023

The liver is a multifunctional organ that plays crucial roles in numerous physiological processes, such as production of bile and proteins for blood plasma, regulation of blood levels of amino acids, processing of hemoglobin, clearance of metabolic waste, maintenance of glucose, etc. Therefore, the liver is essential for the homeostasis of organisms. With the development of research on the liver, there is growing concern about its effect on immune cells of innate and adaptive immunity. For example, the liver regulates the proliferation, differentiation, and effector functions of immune cells through various secreted proteins (also known as “hepatokines”). As a result, the liver is identified as an important regulator of the immune system. Furthermore, many diseases resulting from immune disorders are thought to be related to the dysfunction of the liver, including systemic lupus erythematosus, multiple sclerosis, and heart failure. Thus, the liver plays a role in remote immune regulation and is intricately linked with systemic immunity. This review provides a comprehensive overview of the liver remote regulation of the body’s innate and adaptive immunity regarding to main areas: immune-related molecules secreted by the liver and the liver-resident cells. Additionally, we assessed the influence of the liver on various facets of systemic immune-related diseases, offering insights into the clinical application of target therapies for liver immune regulation, as well as future developmental trends.
Disaster impacts on co-management networks: longitudinal and comparative analysis of Chilean small-scale fisheries
Authors: Marín Andrés , Bodin Örjan , Gelcich Stefan , Castilla Juan Carlos
Published: December 2023

Recent studies have highlighted the relational nature of co-management and investigated which kinds of social network structures define its possibilities to perform, adapt and deal with uncertainty and change. However, there is less understanding about the impacts of disasters and abrupt perturbations on co-management networks. Here we present a social network analysis of the impacts of the 2010 tsunami on co-management in the Chilean fishery. Based on data collected in 21 fisher organizations in the Bio-Bío region, heavily impacted by the tsunami, we assess whether and how co-management facilitating and hindering social relationships have changed after the event, as compared to 16 non-impacted organizations in the Valparaíso region. Baseline data (i.e., 2008) from both regions allows for before-after longitudinal analysis. Our findings show that after the tsunami, co-management networks in Bio-Bío present reduced fragmentation and higher levels of perceived trust among actors in comparison to the non-affected region. A slightly lower tendency towards decentralization was also observed. These findings suggest that post-disaster adjustments have occurred within the same networks. Co-management networks were flexible enough to be rewired as a consequence of abrupt perturbations triggered by the tsunami. Participatory network-based interventions, such as the Chilean MEABR co-management policy, provide a stable and at the same time adaptive setting to respond to coastal disasters.
Detection of tactile-based error-related potentials (ErrPs) in human-robot interaction
Authors: Kim Su Kyoung , Kirchner Elsa Andrea
Published: December 2023

Robot learning based on implicitly extracted error detections (e.g., EEG-based error detections) has been well-investigated in human-robot interaction (HRI). In particular, the use of error-related potential (ErrP) evoked when recognizing errors is advantageous for robot learning when evaluation criteria cannot be explicitly defined, e.g., due to the complex behavior of robots. In most studies, erroneous behavior of robots were recognized visually. In some studies, visuo-tactile stimuli were used to evoke ErrPs or a tactile cue was used to indicate upcoming errors. To our knowledge, there are no studies in which ErrPs are evoked when recognizing errors only via the tactile channel. Hence, we investigated ErrPs evoked by tactile recognition of errors during HRI. In our scenario, subjects recognized errors caused by incorrect behavior of an orthosis during the execution of arm movements tactilely. EEG data from eight subjects was recorded. Subjects were asked to give a motor response to ensure error detection. Latency between the occurrence of errors and the response to errors was expected to be short. We assumed that the motor related brain activity is timely correlated with the ErrP and might be used from the classifier. To better interpret and test our results, we therefore tested ErrP detections in two additional scenarios, i.e., without motor response and with delayed motor response. In addition, we transferred three scenarios (motor response, no motor response, delayed motor response). Response times to error was short. However, high ErrP-classification performance was found for all subjects in case of motor response and no motor response condition. Further, ErrP classification performance was reduced for the transfer between motor response and delayed motor response, but not for the transfer between motor response and no motor response. We have shown that tactilely induced errors can be detected with high accuracy from brain activity. Our preliminary results suggest that also in tactile ErrPs the brain response is clear enough such that motor response is not relevant for classification. However, in future work, we will more systematically investigate tactile-based ErrP classification.
Pea protein hydrolysate reduces blood glucose in high-fat diet and streptozotocin-induced diabetic mice
Authors: Liao Wang , Cao Xinyi , Xia Hui , Wang Shaokang , Chen Liang , Sun Guiju
Published: December 2023

Introduction

Food proteins have been recognized as an ideal source to release bioactive peptides with the potential to intervene nutrition related chronic diseases, such as cardiovascular diseases, obesity and diabetes. Our previous studies showed that pea protein hydrolysate (PPH) could suppress hepatic glucose production in hepatic cells via inhibiting the gluconeogenic signaling. Thus, we hypothesized that PPH could play the hypoglycemic role in vivo.

Methods

In the present study, the mice model with type 2 diabetic mellitus (T2DM) was developed by high-fat diet and low dose of streptozotocin injections. PPH was administered orally with a dosage of 1000 mg/kg body weight for 9 weeks, followed by the downstream biomedical analyses.

Results

The results showed that the 9-week treatment of PPH could reduce fasting blood glucose by 29.6% and improve glucose tolerance in the T2DM mice. The associated mechanisms included suppression of the gluconeogenic pathway, activation of the insulin signaling and modulation of the renin angiotensin system in the liver of the diabetic mice. In addition, the levels of pro-inflammatory markers in both liver and serum were reduced by the PPH treatment.

Conclusion

The hypoglycemic effect of PPH in T2DM mice was demonstrated in the present study. Findings from this study could provide rationale to incorporate PPH into functional foods or nutraceuticals for glycemic control.
Prognostic significance of HALP score and combination of peripheral blood multiple indicators in patients with early breast cancer
Authors: Zhao Zirui , Xu Lingyun
Published: December 2023

Background

To assess the prognostic significance of preoperative hemoglobin, albumin, lymphocyte, and platelet (HALP) score combined with multiple peripheral blood indicators in patients with early breast cancer (EBC).

Methods

A total of 411 patients with early invasive breast cancer underwent breast-conserving surgery or radical surgery at Changzhou No.2 People’s Hospital from January 2015 to December 2020. The cut-off values of HALP, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), lymphocyte-to-monocyte ratio (LMR), and prognostic nutritional index (PNI) were calculated using the software X-tile. The primary outcomes were recurrence-free survival (RFS), which was analyzed using the Kaplan Meier (K-M) method, while log-rank was used to test the differences between high and low curves. Cox regression analysis was used to analyze the prognostic significance of HALP. Furthermore, the prognostic predictive value of independent prognostic factors was determined using the receiver operating characteristic (ROC) curve.

Results

Low HALP score (P<0.0001), high PLR (P<0.0001), and low LMR (P = 0.0345) were significantly associated with worse RFS. Body mass index (BMI)<24 (P = 0.0036), no diabetes (P = 0.0205), earlier TNM stage (P = 0.0005), and no lymph node metastasis (P = 0.0022) were positively correlated with longer survival HALP scores (hazard ratio [HR] 95% confidence interval [CI]: 0.08 (0.024–0.265), P<0.0001), BMI (HR 95%CI: 0.254 (0.109–0.589), P = 0.001), TNM stage (HR 95%CI: 0.153 (0.041–0.571), P = 0.005), and diabetes (HR 95%CI: 0.259 (0.085–0.785), P = 0.017) were demonstrated as independent prognostic factors by Cox regression analysis. The ROC curves depicted that the two most valuable factors were TNM stage and HALP, and combined independent factors were more accurate in prognostic prediction than any single factor. This further indicated that the TNM stage combined HALP or BMI were more valuable combinations.

Conclusion

The HALP score was an independent prognostic factor for EBC and was significantly associated with worse RFS. This score may predict the probability of postoperative tumor recurrence or metastasis before surgery.
Ethyl acetate extract of Terminalia chebula alleviates DSS-induced ulcerative colitis in C57BL/6 mice
Authors: Dong Wan-Rong , Li Yao-Yao , Liu Tian-Tian , Zhou Gao , Chen Yu-Xin
Published: December 2023

Background: The Chinese pharmacopeia records Terminalia chebula as effective in treating prolonged diarrhea and dysentery, blood in the stool, and prolapse. Modern pharmacological research proves it has multiple pharmacological benefits, including antioxidant, anti-inflammatory, analgesic, hepatoprotective, neuroprotective, and other properties.

Objectives: This study aims to clarify the role of Terminalia chebula’s ethyl acetate extract (TCEA) on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice, as well as explore the potential mechanism of action.

Materials and methods: The variation of different extracts of T. chebula was detected using the HPLC technique, and the main components in TCEA were identified. DSS was used to establish a mouse model to mimic the physiological state of UC in humans; the alleviating effect of TCEA and positive control 5-ASA on UC mice were evaluated by gavage treatment. Disease progression was assessed by monitoring the mouse’s weight change and disease activity index (DAI). The changes in colon tissue were estimated by measuring colon length, HE, and AB-PAS staining and detecting oxidative stress parameters. The results draw from Western blot and real-time PCR showed the TLR4/MyD88/NF-κB pathway may involve in the anti-inflammatory activity of TCEA. Furthermore, the gut flora sequencing technique was employed to monitor the differentiation of intestinal microbiota of mice induced by DSS and TCEA treatment.

Results: TCEA significantly lowered DAI scores and inhibited the weight loss and colonic shortening induced by DSS. The colon histomorphology and oxidative stress levels were enhanced after TCEA treatment compared with DSS induced UC group. TCEA attenuated the inflammatory response by regulating TLR4/MyD88/NF-κB pathway activation. Intestinal flora sequencing showed that DSS and TCEA greatly impacted mice’s composition and diversity of intestinal microorganisms. But TCEA increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes and Proteobacteria compared with the DSS group, which contributed a lot to returning the intestinal flora to a balanced state.

Conclusion: This study confirms the alleviating effect of TCEA on UC and provides new ideas for developing TCEA into a new drug to treat UC.
NOP58 induction potentiates chemoresistance of colorectal cancer cells through aerobic glycolysis as evidenced by proteomics analysis
Authors: Wang Feifei , Yu Bin , Yu Quanyong , Wang Guanglin , Li Baokun , Guo Ganlin , Wang Handong , Shen Hui , Li Shujin , Ma Chunling , Jia Xianxian , Wang Guiying , Cong Bin
Published: December 2023

Introduction: The majority of individuals diagnosed with advanced colorectal cancer (CRC) will ultimately acquire resistance to 5-FU treatment. An increasing amount of evidence indicates that aerobic glycolysis performs a significant function in the progression and resistance of CRC. Nevertheless, the fundamental mechanisms remain to be fully understood.

Methods: Proteomic analysis of 5-FU resistant CRC cells was implemented to identify and determine potential difference expression protein.

Results: These proteins may exhibit resistance mechanisms that are potentially linked to the process of aerobic glycolysis. Herein, we found that nucleolar protein 58 (NOP58) has been overexpressed within two 5-FU resistant CRC cells, 116-5FuR and Lovo-5FuR. Meanwhile, the glycolysis rate of drug-resistant cancer cells has increased. NOP58 knockdown decreased glycolysis and enhanced the sensitivity of 116-5FuR and Lovo-5FuR cells to 5FU.

Conclusion: The proteomic analysis of chemoresistance identifies a new target involved in the cellular adaption to 5-FU and therefore highlights a possible new therapeutic strategy to overcome this resistance.
Epidemic spreading with an awareness-based adaptive mechanism in temporal multiplex networks
Authors: Xiong Wenze , Zhou Chunjie
Published: December 2023

Social interaction, which is intricate and time-varying, has emerged as a pivotal consideration in epidemic spreading. In this paper, we devise a UAU–SIS model for simulating awareness diffusion and epidemic spreading on temporal multiplex networks. Drawing inspiration from individuals’ self-protection behaviors, a novel adaptive update mechanism is developed. To provide a more precise representation of the collective social interactions encompassing individuals, we introduce the higher-order network structure encompassing temporal variability. Building upon the established framework of the microscopic Markov chain approach (MMCA) for static networks, we extend its applicability to the condition of temporal networks and derive the threshold within the coupled dynamics. Our extensive simulations illuminate the dual role of awareness in epidemic mitigation. Beyond solely diminishing infection probabilities through self-protective measures, individual awareness additionally facilitates to change the network structure to separate them from the infected. By elucidating these fundamental characteristics, our research contributes to advancing more effective strategies for epidemic mitigation and containment.
Automatic epileptic seizure detection based on persistent homology
Authors: Wang Ziyu , Liu Feifei , Shi Shuhua , Xia Shengxiang , Peng Fulai , Wang Lin , Ai Sen , Xu Zheng
Published: December 2023

Epilepsy is a prevalent brain disease, which is quite difficult-to-treat or cure. This study developed a novel automatic seizure detection method based on the persistent homology method. In this study, a Vietoris–Rips (VR) complex filtration model was constructed based on the EEG data. And the persistent homology method was applied to calculate the VR complex filtration barcodes to describe the topological changes of EEG recordings. Afterward, the barcodes as the topological characteristics of EEG signals were fed into the GoogLeNet for classification. The persistent homology is applicable for multi-channel EEG data analysis, where the global topological information is calculated and the features are extracted by considering the multi-channel EEG data as a whole, without the multiple calculations or the post-stitching. Three databases were used to evaluate the proposed approach and the results showed that the approach had high performances in the epilepsy detection. The results obtained from the CHB-MIT Database recordings revealed that the proposed approach can achieve a segment-based averaged accuracy, sensitivity and specificity values of 97.05%, 96.71% and 97.38%, and achieve an event-based averaged sensitivity value of 100% with 1.22 s average detection latency. In addition, on the Siena Scalp Database, the proposed method yields averaged accuracy, sensitivity and specificity values of 96.42%, 95.23% and 97.6%. Multiple tasks of the Bonn Database also showed achieved accuracy of 99.55%, 98.63%, 98.28% and 97.68%, respectively. The experimental results on these three EEG databases illustrate the efficiency and robustness of our approach for automatic detection of epileptic seizure.
Genome-wide identification of a novel Na+ transporter from Bienertia sinuspersici and overexpression of BsHKT1;2 improved salt tolerance in Brassica rapa
Authors: Irulappan Vadivelmurugan , Park Hyun Woo , Han Sang-Yun , Kim Myung-Hee , Kim Jung Sun
Published: December 2023

Salt stress is an ever-increasing stressor that affects both plants and humans. Therefore, developing strategies to limit the undesirable effects of salt stress is essential. Sodium ion exclusion is well known for its efficient salt-tolerance mechanism. The High-affinity K+ Transporter (HKT) excludes excess Na+ from the transpiration stream. This study identified and characterized the HKT protein family in Bienertia sinuspersici, a single-cell C4 plant. The HKT and Salt Overly Sensitive 1 (SOS1) expression levels were examined in B. sinuspersici and Arabidopsis thaliana leaves under four different salt stress conditions: 0, 100, 200, and 300 mM NaCl. Furthermore, BsHKT1;2 was cloned, thereby producing stable transgenic Brassica rapa. Our results showed that, compared to A. thaliana as a glycophyte, the HKT family is expanded in B. sinuspersici as a halophyte with three paralogs. The phylogenetic analysis revealed three paralogs belonging to the HKT subfamily I. Out of three copies, the expression of BsHKT1;2 was higher in Bienertia under control and salt stress conditions than in A. thaliana. Stable transgenic plants overexpressing 35S::BsHKT1;2 showed higher salt tolerance than non-transgenic plants. Higher biomass and longer roots were observed in the transgenic plants under salt stress than in non-transgenic plants. This study demonstrates the evolutionary and functional differences in HKT proteins between glycophytes and halophytes and associates the role of BsHKT1;2 in imparting salt tolerance and productivity.
Impact of Farmer Participation in Production Chain Outsourcing Services on Agricultural Output Level and Output Risk: Evidence from the Guanzhong Plain, China
Authors: Xie Shouhong , Zhang Jizhou , Li Xiaojing , Chen Zhe , Zhang Xiaoning , Xia Xianli
Published: December 2023

Shifting from a land-scale operation to a service-scale operation of agricultural production chain outsourcing services (APOS) is crucial to achieving innovation in agricultural-scale operation techniques. Using propensity score matching (PSM) and data from 1027 farm households in Guanzhong Plain, Shaanxi Province, we empirically assessed the impact of APOS on agricultural output level and output risk. First, age, gender, health, education, training, number of outworkers, land tenure, land contiguity, and subsidy satisfaction had a substantial beneficial influence on the involvement of farm families in APOS. Second, involvement in APOS may greatly increase the amount of agricultural production and lower the risk associated with farm families’ agricultural output. Moreover, the participation in outsourcing services for agricultural machinery use and field management significantly increased agricultural output and decreased output risk, but the participation in agricultural machinery use outsourcing services increased yield and reduced risks more significantly.
Monitoring of Chemical and Fermentative Characteristics during Different Treatments of Grape Pomace Silage
Authors: Sokač Cvetnić Tea , Gunjević Veronika , Damjanović Anja , Pušek Anita , Jurinjak Tušek Ana , Jakovljević Tamara , Radojčić Redovniković Ivana , Uher Darko
Published: December 2023

Grape pomace is a fibrous food with satisfactory quantities of residual sugars. It meets the desirable characteristics for conservation in the form of silage for later use in animal feed, mainly for ruminant herbivores. Fresh grape pomace was subdivided into three treatment groups: grape pomace as a control, grape pomace treated with an inoculum of lactic acid bacteria, and grape pomace treated with zeolite. The treatments were performed in micro-silos over 90 days. There was a significant change (p < 0.05) in the chemical characteristics, content of biologically active compounds, and fermentative characteristics during the silage of all treatments. After 30, 60 and 90 days of ensiling, silages treated with inoculum and zeolite had better fermentation quality indicated by significantly (p < 0.05) lower pH and ammonia-nitrogen contents compared with those of the control. Also, the additives have decreased the total polyphenols and tannins for 97% in average which confirmed that lactic acid bacteria and zeolite positively effect on the degradation of polyphenols and tannins in grape pomace silage. The Flieg score was calculated and the values were above 80% what refers to excellent silage. In conclusion, our results suggest that inoculant and zeolite supplementation improves the quality of grape pomace silage for later use in animal feed.
Physiological Characteristics, Crop Growth and Grain Yield of Twelve Wheat Varieties Cultivated in the North China Plain
Authors: Tang Xiaopei , Liu Haijun , Zhang Wenjie
Published: December 2023

Climate change and water resource shortages have become important problems limiting winter wheat production in the North China Plain (NCP). Understanding the physiological characteristics of different wheat varieties and their relationship with crop growth and yield is of great importance for addressing climate change through a scientific approach, adopting reliable wheat varieties, and ensuring food production. This study was conducted throughout three winter wheat seasons from 2018 to 2021. The crop growth, physiological indicators, crop yields, and water productivity (WP) of 12 wheat varieties widely cultivated in the NCP were measured to investigate the relationships between physiological characteristics and crop yield. The results showed that among the three wheat seasons, the maximum plant height of each wheat variety was relatively stable, while the changes in maximum plant density (PDm), maximum leaf area index (LAIm), and maximum dry matter (DMm) were highly variable. The gas exchange parameters and fluorescence parameters of wheat flag leaves varied with growth stage, and certain varieties were sensitive to water stress. The wheat grain yield, seasonal crop evapotranspiration (ETa), and WP of the 12 varieties were similar in the 2019–2020 and 2020–2011 seasons and were approximately 25%, 7%, and 19% higher than those in the 2018–2019 season, respectively, in which there were consecutive cool events in the winter and spring. Generally, the grain yields were positively correlated with PDm, LAIm, DMm, ETa, the harvest index, and WP, at a significance level of 0.01. The high-yield wheat varieties had higher photosynthetic rates in the middle and late grain-filling stages. Based on these relationships, high-yield wheat varieties may be selected to address climate change in the NCP as well as other regions in the world. Considering the variations in yield and WP in the three different climatic conditions, we recommend Yingbo700, Lunxuan103, Shimai26, Shinong086, Han6172, and Hanong1412 as high-yield and drought-resistant wheat varieties to be used in the NCP.
Optimizing Soil Management for Sustainable Viticulture: Insights from a Rendzic Leptosol Vineyard in the Nitra Wine Region, Slovakia
Authors: Šimanský Vladimír , Wójcik-Gront Elżbieta , Jonczak Jerzy , Horák Ján
Published: December 2023

Properly chosen soil management practices can stabilize the nutrient regime in the soil, including the mobility and bioavailability of hazardous elements. This study aimed to identify the optimal soil management practices in a productive vineyard on Rendzic Leptosol in the Nitra wine region (Slovakia). Soil samples were collected each spring from two depths, 0–30 cm, and 30–60 cm, with the following treatments: T—soil tillage, P + FYM—plowed farmyard manure, G—grass strips, G + NPK1—first-level fertilization, and G + NPK2—second-level fertilization, from 2019 to 2023. The results indicated that more pronounced changes in soil properties occurred in the 0–30 cm layer. Higher NPK rates significantly affected soil sorption capacity and decreased soil pH when compared to other treatments. While G + NPK2 showed the highest storage of total N, S, P, K, and available P and K, it exhibited the lowest levels of total and available Ca. The T treatment displayed the lowest storage of C, N, S, P, and available K. In terms of hazardous metals (Cr, Ni, Pb, and Zn) none exceeded the limiting values in any of the soil management practices. However, in the 0–30 cm layer, Cu concentrations exceeded the limits set by Slovak Republic regulations in the T, P + FYM, G, G + NPK1, and G + NPK2 treatments by 62.6, 73.7, 70.2, 82.1, and 102.9 mg kg−1, respectively. Additionally, as total C increased, Cr concentration was observed to decrease with correlation (r = −0.46). Positive correlations were found between total C and Zn, as well as CaCO3 and Zn in the 0–30 layer.
Correction: Carota et al. Neuroprotective Role of α-Lipoic Acid in Iron-Overload-Mediated Toxicity and Inflammation in In Vitro and In Vivo Models. Antioxidants 2022, 11, 1596
Authors: Carota Giuseppe , Distefano Alfio , Spampinato Mariarita , Giallongo Cesarina , Broggi Giuseppe , Longhitano Lucia , Palumbo Giuseppe A. , Parenti Rosalba , Caltabiano Rosario , Giallongo Sebastiano , Di Rosa Michelino , Polosa Riccardo , Bramanti Vincenzo , Vicario Nunzio , Li Volti Giovanni , Tibullo Daniele
Published: December 2023

In the original publication [...]
17β-Estradiol (E2) Upregulates the ERα/SIRT1/PGC-1α Signaling Pathway and Protects Mitochondrial Function to Prevent Bilateral Oophorectomy (OVX)-Induced Nonalcoholic Fatty Liver Disease (NAFLD)
Authors: Tian Ying , Hong Xinyu , Xie Yuan , Guo Zaixin , Yu Qi
Published: December 2023

Premature menopause is associated with an increased prevalence of nonalcoholic fatty liver disease (NAFLD). Menopausal hormone therapy (MHT) has been widely used in clinical practice and has the potential to protect mitochondrial function and alleviate NAFLD. After bilateral oophorectomy (OVX), female rats without 17β-estradiol (E2) intervention developed NAFLD, whereas E2 supplementation was effective in preventing NAFLD in female rats. The altered pathways and cellular events from both comparison pairs, namely, the OVX vs. sham group and the OVX vs. E2 group, were assessed using transcriptomic analysis. KEGG pathways enriched by both transcriptomic and metabolomic analyses strongly suggest that oxidative phosphorylation is a vital pathway that changes during the development of NAFLD and remains unchanged when E2 is applied. Liver tissue from the OVX-induced NAFLD group exhibited increased lipid peroxidation, impaired mitochondria, and downregulated ERα/SIRT1/PGC-1α expression. An in vitro study indicated that the protective effect of E2 treatment on hepatic steatosis could be abolished when ERα or SIRT1 was selectively inhibited. This damage was accompanied by reduced mitochondrial complex activity and increased lipid peroxidation. The current research indicates that E2 upregulates the ERα/SIRT1/PGC-1α signaling pathway and protects mitochondrial function to prevent OVX-induced NAFLD.
Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from Physalis alkekengi L.
Authors: Crescenzi Maria Assunta , Serreli Gabriele , Deiana Monica , Tuberoso Carlo I. G. , Montoro Paola , Piacente Sonia
Published: December 2023

Due to the increasing use of Physalis alkekengi L. as a food supplement and starting material for tea preparation, a comprehensive analysis of green extracts was performed. Two different extraction methods were applied to yellow Physalis alkekengi L. fruit and calyx and compared: hydroalcoholic extraction and decoction. Characterization of the metabolome of the calyx and fruit of yellow Physalis alkekengi L. was performed by LC-ESI/LTQOrbitrap/MS followed by LC-ESI/LTQOrbitrap/MS/MS to identify 58 phytocompounds using the two different extraction techniques. Subsequently, through preliminary spectrophotometric assays followed by cell studies, the antioxidant activity of the different Physalis alkekengi L. extracts were evaluated. It was found that Physalis alkekengi L. extracts are a good source of metabolites such as flavonoids, organic acids, phenylpropanoids, physalins and carotenoids, with various biological activities, in particular, antioxidant activity capable of reducing the production of free radicals in intestinal Caco-2 cells. For the first time, an integrated approach (metabolomics approach and antioxidant evaluation) was applied to the study of Physalis alkekengi green extracts and decoctions, the green extraction method mostly used in herbal preparations. An interesting finding was the high antioxidant activity shown by these extracts.
Roasting Extract of Handroanthus impetiginosus Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells
Authors: Nahar Jinnatun , Morshed Md Niaj , Rupa Esrat Jahan , Lee Jung Hyeok , Kariyarath Valappil Anjali , Awais Muhammad , Hun Ko Jeong , Sook Lee Ji , Al-Amin Md. , Ahn Jong Chan , Yang Deok Chun , Jung Seok-Kyu
Published: December 2023

The family Bignoniaceae includes Handroanthus impetiginosus trees, which are sparsely distributed in the northeast of Brazil. Natural products play a vital role in the discovery of drugs for various diseases. Many plants have been used as sources of medicines because of their chemical diversity and potent bioactivity. Handroanthus impetiginosus has been used traditionally to cure a wide range of illnesses, such as cancer, oxidative stress, and inflammation. This work highlights the cytotoxicity, cell death, and routes of apoptosis in lung cancer cells (A549) and the anti-inflammatory and antioxidant effects of roasted Handroanthus impetiginosus (lapacho/taheebo) in normal cells. The cell viability assay indicated that puffing roasted taheebo is nontoxic to a normal cell line up to 500 µg/mL but significantly toxic to A549 cells. The roasted lapacho/taheebo also increases reactive oxygen species (ROS) generation in A549 lung cancer cells, and cellular apoptosis via a mitochondrial intrinsic pathway was confirmed. The roasted lapacho/taheebo significantly inhibited both colony formation and cell migration ability, highlighting its potential as an anticancer agent. Additionally, this study demonstrates that roasted taheebo enhanced the expression of genes for BAX accumulation and decreased Bcl-2 gene expression through the p53 signaling pathway. Furthermore, research on the anti-inflammatory properties of roasted taheebo revealed a strong NO inhibition as well as the inhibition of inflammatory mediators (TNF-α, iNOS, COX-2, IL-6, and IL-8) through the NF-κB signaling pathway. However, in H2O2-induced HaCaT cells, roasted taheebo extract significantly reduced oxidative stress by upregulating the level of expression of antioxidative markers (SOD, CAT, GPx, and GST) at 50 μg/mL. As a result, roasted taheebo justifies investigation in animal and clinical trials as a possible source of antioxidants, anti-inflammatory substances, and anti-cancer compounds.