bluuebunny/crossref_metadata_2025 huggingface dataset:
An Observational Study on the Molecular Profiling of Primary Melanomas Reveals a Progression Dependence on Mitochondrial Activation
Authors: Gil Jeovanis , Rezeli Melinda , Lutz Elmar G. , Kim Yonghyo , Sugihara Yutaka , Malm Johan , Semenov Yevgeniy R. , Yu Kun-Hsing , Nguyen Nga , Wan Guihong , Kemény Lajos V. , Kárpáti Sarolta , Németh István Balázs , Marko-Varga György
Published: December 2021

Melanoma in advanced stages is one of the most aggressive tumors and the deadliest of skin cancers. To date, the histopathological staging focuses on tumor thickness, and clinical staging is a major estimate of the clinical behavior of primary melanoma. Here we report on an observational study with in-depth molecular profiling at the protein level including post-translational modifications (PTMs) on eleven primary tumors from melanoma patients. Global proteomics, phosphoproteomics, and acetylomics were performed on each sample. We observed an up-regulation of key mitochondrial functions, including the mitochondrial translation machinery and the down-regulation of structural proteins involved in cell adhesion, the cytoskeleton organization, and epidermis development, which dictates the progression of the disease. Additionally, the PTM level pathways related to RNA processing and transport, as well as chromatin organization, were dysregulated in relation to the progression of melanoma. Most of the pathways dysregulated in this cohort were enriched in genes differentially expressed at the transcript level when similar groups are compared or metastasis to primary melanomas. At the genome level, we found significant differences in the mutation profiles between metastatic and primary melanomas. Our findings also highlighted sex-related differences in the molecular profiles. Remarkably, primary melanomas in women showed higher levels of antigen processing and presentation, and activation of the immune system response. Our results provide novel insights, relevant for developing personalized precision treatments for melanoma patients.
The Role of Arginine Metabolism in Oral Tongue Squamous Cell Carcinoma
Authors: Leung Leanne Lee , Lau Nicolas Cheuk Hang , Liu Jiaxun , Qu Xinyu , Tsui Stephen Kwok-Wing , Hou Jinpao , Law Cherie Tsz-Yiu , Ng Tung Him , Yam Judy Wai Ping , Chow Chit , Chan Amy B. W. , Chan Jason Y. K. , Meehan Katie
Published: December 2021

Early diagnosis and treatment do not prevent the high morbidity and poor prognosis of oral tongue squamous cell carcinoma (TSCC). Earlier studies have shown that ARG1 signaling is deregulated in TSCC. Here, we investigated the complexity of ARG1 metabolism in this cancer subsite to appreciate the therapeutic potential of this potential biological vulnerability. Various functional studies show that ARG1 overexpression in oral cancer cells inhibits cell proliferation and invasion compared with controls. Further, RNA-sequencing revealed numerous differentially expressed genes (DEGs) and associated networks were dysregulated by ARG1 overexpression, including hypoxia-inducible factor (HIFα) signaling, the natural killer cell signaling pathway and interferon signaling. Our work provides a foundation for understanding the mechanism of action of disrupted arginine metabolism in oral tongue squamous cell carcinoma. This may impact the community for developing further therapeutic approaches.
Photodegradation of Carbol Fuchsin Dye Using an Fe2−xCuxZr2−xWxO7 Photocatalyst under Visible-Light Irradiation
Authors: Abu-Zurayk Rund , Khalaf Aya , Abbas Hussien A. , Nasr Rabab A. , Jamil Tarek S. , Al Bawab Abeer
Published: December 2021

Fe2−xCuxZr2−xWxO7 (x: 0, 0.05, 0.015) nanoparticles were synthesized following the Pechini method and characterized via X-ray diffraction (XRD), transmission electron microscopy (TEM), and diffuse reflectance spectroscopy (DRS) measurements to be used as photocatalysts in colored water remediation. All of the prepared materials were crystallized in a cubic fluorite phase as the major phase. The band gap was reduced upon doping with W6+ and Cu2+ from 1.96 eV to 1.47 eV for Fe1.85Cu0.15Zr1.85W0.15O7. Carbol fuchsin (CF) dye was used to determine the photocatalytic degradation efficiency of the prepared catalysts. Degradation efficiency was directly proportional to the dopant’s concentration. Complete removal of 20 mg/L CF was achieved under optimal conditions (pH 9, and catalyst loading of 1.5 g/L) using Fe1.85Cu0.15Zr1.85W0.15O7. The degradation rate followed pseudo-first-order kinetics. The reusability for photocatalysts was tested five times, decreasing its efficiency by 4% after the fifth cycle, which indicates that the prepared Fe1.85Cu0.15Zr1.85W0.15O7 photocatalyst is a promising novel photocatalyst due to its superior efficiency in dye photodegradation.
Lauric Acid, a Dietary Saturated Medium-Chain Fatty Acid, Elicits Calcium-Dependent Eryptosis
Authors: Alfhili Mohammad A. , Aljuraiban Ghadeer S.
Published: December 2021

Cardiovascular diseases (CVD) are a leading cause of mortality worldwide, and dietary habits represent a major risk factor for dyslipidemia; a hallmark of CVD. Saturated fatty acids contribute to CVD by aggravating dyslipidemia, and, in particular, lauric acid (LA) raises circulating cholesterol levels. The role of red blood cells (RBCs) in CVD is increasingly being appreciated, and eryptosis has recently been identified as a novel mechanism in CVD. However, the effect of LA on RBC physiology has not been thoroughly investigated. RBCs were isolated from heparin-anticoagulated whole blood (WB) and exposed to 50–250 μM of LA for 24 h at 37 °C. Hemoglobin was photometrically examined as an indicator of hemolysis, whereas eryptosis was assessed by Annexin V-FITC for phosphatidylserine (PS) exposure, Fluo4/AM for Ca2+, light scatter for cellular morphology, H2DCFDA for oxidative stress, and BODIPY 581/591 C11 for lipid peroxidation. WB was also examined for RBC, leukocyte, and platelet viability and indices. LA caused dose-responsive hemolysis, and Ca2+-dependent PS exposure, elevated erythrocyte sedimentation rate (ESR), cytosolic Ca2+ overload, cell shrinkage and granularity, oxidative stress, accumulation of lipid peroxides, and stimulation of casein kinase 1α (CK1α). In WB, LA disrupted leukocyte distribution with elevated neutrophil-lymphocyte ratio (NLR) due to selective toxicity to lymphocytes. In conclusion, this report provides the first evidence of the pro-eryptotic potential of LA and associated mechanisms, which informs dietary interventions aimed at CVD prevention and management.
Parkin beyond Parkinson’s Disease—A Functional Meaning of Parkin Downregulation in TDP-43 Proteinopathies
Authors: Gaweda-Walerych Katarzyna , Sitek Emilia Jadwiga , Narożańska Ewa , Buratti Emanuele
Published: December 2021

Parkin and PINK1 are key regulators of mitophagy, an autophagic pathway for selective elimination of dysfunctional mitochondria. To this date, parkin depletion has been associated with recessive early onset Parkinson’s disease (PD) caused by loss-of-function mutations in the PARK2 gene, while, in sporadic PD, the activity and abundance of this protein can be compromised by stress-related modifications. Intriguingly, research in recent years has shown that parkin depletion is not limited to PD but is also observed in other neurodegenerative diseases—especially those characterized by TDP-43 proteinopathies, such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Here, we discuss the evidence of parkin downregulation in these disease phenotypes, its emerging connections with TDP-43, and its possible functional implications.
Hypomineralized Primary Teeth in Preterm Low Birth Weight Children and Its Association with Molar Incisor Hypomineralization—A 3-Year-Prospective Study
Authors: Noor Mohamed Roshan , Basha Sakeenabi , Virupaxi Shruti Girish , Idawara Eregowda Neena , Parameshwarappa Poornima
Published: December 2021

The present prospective study was conducted to assess the prevalence of enamel hypomineralization (EH) in primary dentition among preterm low birth weight (PT-LBW) children, incidence of molar incisor hypomineralization (MIH) in the same cohorts, and to determine associations between PT-LBW, hypomineralization in primary second molars, and MIH. A total of 287 PTLBW study subjects and 290 control full-term normal birth weight subjects were followed up for 36 months. Enamel defects were recorded at baseline. The same cohorts were examined after 3 years for MIH using the European Academy of Paediatric Dentistry (EAPD) criteria. Multiple variable logistic regression models were developed. A total of 279 children (48.4%) presented with EH in primary dentition and 207 (35.9%) children presented with MIH. Children with primary second molar hypomineralization had 2.13 (R2 = 0.19, 95% CI = 0.98–4.19, p = 0.005) times higher frequency of MIH. Children with PT-LBW had 3.02 times (R2 = 0.31, 95% CI = 1.01–5.94, p = 0.005) higher frequency of MIH incidence after adjusting for childhood infection, prenatal history, and presence of hypomineralized primary second molars. To conclude, the present study showed significant association between PT-LBW, hypomineralized second primary molars, and incidence of MIH.
Lexical and Grammatical Errors in Developmentally Language Disordered and Typically Developed Children: The Impact of Age and Discourse Genre
Authors: Kornev Aleksandr N. , Balčiūnienė Ingrida
Published: December 2021

Persistent lexical and grammatical errors in children’s speech are usually recognized as the main evidence of language delay or language disorder. These errors are usually treated as a sign of a deficit in language competence. On the other hand, some studies have revealed the same kinds of grammatical errors in children with developmental language disorder (DLD) and in typically developed (TD) children. Quite often, DLD children use grammatical markers properly, but sometimes they do this erroneously. It has been suggested that the main area of the limitations in DLD children is language performance but not language competence. From the perspective of the resource deficit model, the error rate in DLD children should be influenced by the cognitive demands of utterance and text production. We presume that different genres of discourse demand a different number of cognitive resources and, thus, should differently impact the error rate in children’s speech production. To test our hypothesis, we carried out an error analysis of two corpora of child discourse. The first corpus contained longitudinal data of discourse (personal narratives, fictional stories, chats, and discussions) collected from 12 children at four age points (4 years 3 months., 4 years 8 months., 5 years 3 months., and 5 years 9 months. years). Another corpus contained discourse texts (fictional stories and discussions) collected in the framework of a cross-sectional study from 6-year-old TD and DLD children; the DLD children had language expression but not comprehension difficulties. A comparative analysis between different discourse genres evidenced that the genre of discourse and age of assessment impacted the error distribution in the DLD and TD children. Such variables as the lexical and morphological error rates were impacted the most significantly. The results of the two studies confirmed our hypothesis regarding the probabilistic nature of lexical and grammatical errors in both DLD and TD children and the relationship between a cognitive loading of the genre and the error rate.
De Novo Assembly and Species-Specific Marker Development as a Useful Tool for the Identification of Scutellaria L. Species
Authors: Choi Hakjoon , Kang Wan Seok , Kim Jin Seok , Na Chang-Su , Kim Sunoh
Published: December 2021

Scutellaria L. (family Lamiaceae) includes approximately 470 species found in most parts of the world and is commonly known as skullcaps. Scutellaria L. is a medicinal herb used as a folk remedy in Korea and East Asia, but it is difficult to identify and classify various subspecies by morphological methods. Since Scutellaria L. has not been studied genetically, to expand the knowledge of species in the genus Scutellaria L., de novo whole-genome assembly was performed in Scutellaria indica var. tsusimensis (H. Hara) Ohwi using the Illumina sequencing platform. We aimed to develop a molecular method that could be used to classify S.indica var. tsusimensis (H. Hara) Ohwi, S. indica L. and three other Scutellaria L. species. The assembly results for S.indica var. tsusimensis (H. Hara) Ohwi revealed a genome size of 318,741,328 bp and a scaffold N50 of 78,430. The assembly contained 92.08% of the conserved BUSCO core gene set and was estimated to cover 94.65% of the genome. The obtained genes were compared with previously registered Scutellaria nucleotide sequences and similar regions using the NCBI BLAST service, and a total of 279 similar nucleotide sequences were detected. By selecting the 279 similar nucleotide sequences and nine chloroplast DNA barcode genes, primers were prepared so that the size of the PCR product was 100 to 1000 bp. As a result, a species-specific primer set capable of distinguishing five species of Scutellaria L. was developed.
Very High Cycle Fatigue Properties of 18CrNiMo7-6 Carburized Steel with Gradient Hardness Distribution
Authors: Yang Shaopeng , Cheng Peifeng , Hu Fangzhong , Yu Wenchao , Zhang Chi , Wang Kaizhong , Wang Maoqiu
Published: December 2021

As research of the high cycle fatigue of carburized gear steel could not meet the status quo of longer and longer service lives, research of very high cycle fatigue (VHCF) performance has become the focus of current research. The VHCF properties of case-hardening steel 18CrNiMo7-6 after being carburized with gradient hardness distribution were investigated by means of ultrasonic fatigue tests. The results showed that the carburized specimens with a case hardness of 705 HV and core hardness of 530 HV showed VHCF phenomenon, and the fatigue lives continuously increased to even 109 cycles as the stress amplitude decreased to about 500 MPa. Observations of the fracture surfaces of the fatigue specimens showed that the fatigue crack initiation sites were located in the transition area with the hardness at about 580 HV. It was found that the transition area had low VHCF properties, since the core did not show VHCF phenomenon, and the case had a higher hardness. A fine microstructure was observed in the granular bright facet (GBF) area, and the stress intensity factor ΔKGBF was measured to be 3.04 MPam−1/2. The 109 cycles fatigue life was predicted based on the inclusion size, and the 1010 cycles fatigue life was 490 MPa based on the prediction model.
Reactive Air Brazing of TiAl Alloy Using Ag-CuO: Microstructure and Joint Properties
Authors: Yang Haoran , Si Xiaoqing , Li Chun , Cao Jian
Published: December 2021

TiAl alloy was successfully brazed with Ag-CuO filler in air atmosphere under simple technical conditions. The wettability of a series of Ag-CuO fillers on TiAl was analyzed. Ag-2mol%CuO filler possessed good wetting behavior on TiAl alloy. The microstructure and mechanical properties of the brazed joints were investigated. Oxide layers can be found on both sides, which can be divided into external TiO2-rich layer and internal Al2O3-rich layer. The maximum shear strength of the joint was obtained at 1020 °C holding for 20 min.
Lessons of 15,000 Years of Human–Wildlife Interaction for Conservation in Patagonia in the 21st Century
Authors: Novaro Andrés J. , Walker Rebecca Susan
Published: December 2021

Humans arrived in the Patagonia region of southern South America in the late Pleistocene period, ca. 15,000 years ago. A few centuries later, during a period of rapid warming, the megafauna went extinct in Patagonia, as well as some smaller species, like the guanaco (Lama guanicoe), in the southern portion of the region. As in other regions, extinctions probably occurred due to a combination of effects of climate and direct and indirect impacts of humans on wildlife communities. We reviewed recent archeological and genetic-based discoveries about numbers and distributions of humans and wildlife and their early interactions and used them to draw lessons for current debates among managers and scientists. Recent discoveries, for example, help us understand (1) the population limitation mechanisms and other interactions involving guanacos, livestock, forage, predators, and scavengers; (2) the magnitude of wildlife movements and the need for landscape-level planning for conservation; (3) the importance of indirect effects of human activities on wildlife communities; and (4) the compounded effects of human activities and climate change on wildlife. We believe these lessons drawn from deep time and recent history can help define new priorities for research and management and inform our conservation vision for the 21st century, a period when dramatic climate change impacts will add challenges to a region subject to a century of overgrazing and other anthropogenic pressures.
WT1 and Cyclin D1 Immunohistochemistry: A Useful Adjunct for Diagnosis of Pediatric Small Round Blue Cell Tumors on Small Biopsies
Authors: Salvatorelli Lucia , Parenti Rosalba , Broggi Giuseppe , Vecchio Giada Maria , Angelico Giuseppe , Puzzo Lidia , Di Cataldo Andrea , Di Benedetto Vincenzo , Alaggio Rita , Magro Gaetano
Published: December 2021

Pediatric small round blue cell tumors (SRBCTs) are a heterogeneous group of neoplasms with overlapping morphological appearance. Accordingly, their diagnosis is one of the most difficult in the field of surgical pathology. The most common tumors include rhabdomyosarcoma, Ewing’s sarcoma, neuroblastoma, lymphoblastic lymphoma and Wilms’ tumor (the blastemal component). Over time their diagnosis has become more difficult due to the increasing use of small biopsies. However, the advent of immunohistochemistry has improved the quality of diagnosis in most cases by the application of an adequate panel of immunomarkers. Recently, WT1 and Cyclin D1 have been shown to be useful in the differential diagnosis of SRBCTs on surgically-resected specimens, showing a diffuse cytoplasmic positivity of the former in all RMSs and a diffuse nuclear staining of the latter in both EWS and NB. The aim of the present study was to investigate the expression of WT1 and Cyclin D1 on small biopsies from a series of 105 pediatric SRBCTs to evaluate their diagnostic utility. Both immunomarkers were differentially expressed, with a diffuse and strong cytoplasmic staining for WT1 limited to all cases of RMS, and a diffuse nuclear staining for cyclin D1 restricted to all cases of EWS and NB. Notably, the expression of WT1 and cyclin D1 was also retained in those cases in which the conventional tumor markers (myogenin, desmin and MyoD1 for RMS; CD99 for EWS; NB84 for NB) were focally expressed or more rarely absent. The present study shows that WT1 and Cyclin D1 are helpful immunomarkers exploitable in the differential diagnosis of pediatric SRBCTs on small biopsies, suggesting their applicability in routine practice.
Numerical Modeling of the Wave-Plate-Current Interaction by the Boundary Element Method
Authors: Akarni Hasna , El Aarabi Laila , Mouakkir Laila , Mordane Soumia
Published: December 2021

The aim of this work is to propose a numerical study of the interaction of a wave-horizontal plate fixed and completely immersed in a flat-bottomed tank with a uniform current flowing in the same direction as the incident wave. We investigate in particular the effect of the plate at minimizing the impact of the wave on the coast of beaches by studying the free surface elevation and the reflection coefficient, as well as the influence of the various geometrical parameters on the latter, taking into account the presence of the current. The numerical method used in this study is the boundary element method (BEM), and the results obtained will be confronted with experimental and analytical data existing in the literature.
Control of Foodborne Staphylococcus aureus by Shikonin, a Natural Extract
Authors: Wan Yangli , Wang Xiaowen , Zhang Pengfei , Zhang Meng , Kou Mingying , Shi Chao , Peng Xiaoli , Wang Xin
Published: December 2021

Foodborne Staphylococcus aureus (S. aureus) has attracted widespread attention due to its foodborne infection and food poisoning in human. Shikonin exhibits antibacterial activity against a variety of microorganisms, but there are few studies on its antibacterial activity against S. aureus. This study aims to explore the antibacterial activity and mechanism of shikonin against foodborne S. aureus. The results show that the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of shikonin were equal for all tested strains ranging from 35 μg/mL to 70 μg/mL. Shikonin inhibited the growth of S. aureus by reducing intracellular ATP concentrations, hyperpolarizing cell membrane, destroying the integrity of cell membrane, and changing cell morphology. At the non-inhibitory concentrations (NICs), shikonin significantly inhibited biofilm formation of S. aureus, which was attributed to inhibiting the expression of cidA and sarA genes. Moreover, shikonin also markedly inhibited the transcription and expression of virulence genes (sea and hla) in S. aureus. In addition, shikonin has exhibited antibacterial ability against both planktonic and biofilm forms of S. aureus. Importantly, in vivo results show that shikonin has excellent biocompatibility. Moreover, both the heat stability of shikonin and the antimicrobial activity of shikonin against S. aureus were excellent in food. Our findings suggest that shikonin are promising for use as a natural food additive, and it also has great potential in effectively controlling the contamination of S. aureus in food and reducing the number of illnesses associated with S. aureus.
Synergy of Nitric Oxide and 1-Methylcyclopropene Treatment in Prolong Ripening and Senescence of Peach Fruit
Authors: Wu Xiaoqin , Yuan Jiawei , Wang Xiaoqing , Yu Mingliang , Ma Ruijuan , Yu Zhifang
Published: December 2021

Peach is a putrescible fruit thus drastically restricting its postharvest storage life. In recent years, the application of 1-methylcyclopropene (1-MCP) and nitric oxide (NO) in postharvest fruit quality control has received considerable attention and investigative efforts due to the advantages of using relatively low concentrations and short-time treatment duration. In the present study, the effects of various 1-MCP and NO treatments on peach fruit (Prunus persica L. cv. Xiahui-8) stored at 25 °C were evaluated and compared. Results indicated that the combination treatment with both chemical agents (MN) was most effective in postponing peach ripening and preserving fruit quality, followed by 1-MCP and NO treatment alone. We also demonstrated that NO could delay fruit senescence mainly by stimulating antioxidant enzymes, while 1-MCP overly outperformed NO in the treatment of ‘Xiahui-8′ peach in slowing down respiration rate, inhibiting ethylene production, maintaining high firmness and reducing ROS content. NO treatment showed a greater influence on phenolic compounds than 1-MCP especially anthocyanins, flavanones and flavones according to LC/MS analysis. The phenolic change in MN group were highly associated to NO treatment. Through this study we provide informative physiological, biochemical and molecular evidence for the beneficial effects of the combined 1-MCP and NO treatment on peach fruit based on a functional synergy between these two chemical agents.
Enhancement of Anti-Proliferative Activity of the Extracts from Dehulled Adlay by Fermentation with Bacillus subtilis
Authors: Wen Anyan , Zhu Yong , Mazhar Muhammad , Qin Likang , Zeng Haiying , Zhu Yi
Published: December 2021

Dehulled adlay was fermented with Bacillus subtilis BJ3-2, the anti-proliferative activities of the extracts from fermented dehulled adlay were investigated with six types of tumor cells, and then the bioactive components and the anti-proliferative mechanism were primarily explored. Results showed that all the extracts of B. subtilis-fermented dehulled adlay (BDA) and dehulled adlay (DA) had no inhibition effect on human embryonic kidney 239T cells. The anti-proliferative activities of the extracts from BDA against six types of tumor cells were almost always significantly higher than DA. Compared with others, the n-butanol extract of BDA (BDA-Nb) exhibited stronger anti-proliferative activities against human leukemia K562 cells and human non-small cell lung cancer A549 cells. Importantly, the anti-proliferative activity of fermented dehulled adlay against K562 cells was firstly discovered. Meanwhile, BDA-Nb was rich in tetramethylpyrazine, γ-aminobutyric acid, protocatechuic, 2,3,4-trihydroxybenzoic, chlorogenic, p-hydroxybenzoic, caffeic, trans-cinnamic, ferulic acids, and rutin. BDA-Nb induced the proliferative inhibition of K562 and A549 cells due to abnormal cell morphology, the increased cell population in G1 phase and apoptosis rate, the downregulation of Bcl-2, and the upregulation of Bax and caspase-3/8/9. These results indicate that dehulled adlay fermented with B. subtilis could be a potential therapeutic agent for leukemia and lung cancer.
Use of Biotechnological Methods to Support the Production of New Peach Hybrids
Authors: Mitrofanova Irina , Lesnikova-Sedoshenko Nina , Tsiupka Valentina , Smykov Anatoliy , Mitrofanova Olga
Published: December 2021

Peach [Prunus persica (L.) Batsch] is among the most demanded fruit crops in the world. Biotechnological methods help to originate new hybrid forms in order to increase the cultivar diversity and create new valuable genotypes. Cross combinations between the cultivars Clyde Wilson, Jerseyglo, Loadel, Summerglo and the promising cultivar ‘Nikitskiy Podarok’ have been done. The embryos of these hybrids germinated and formed plantlets after stratification at 4 °C for 45–60 days. The best regeneration rates in the hybrids ‘Loadel’ × ‘Nikitskiy Podarok’ and ‘Summerglo’ × ‘Nikitskiy Podarok’ (96.30% and 92.59%, respectively) were noted on hormone-free Monnier culture medium supplemented with 400.0 mg L−1 casein hydrolyzate. When the newly formed plantlets had necrosis of the shoot apex or immature roots, nodal shoot segments were used. At the same time, a high regeneration capacity was noted in the hybrids ‘Summerglo’ × ‘Nikitskiy Podarok’ and ‘Loadel’ × ‘Nikitskiy Podarok’ on B5 culture medium with 0.75 mg L−1 6–benzyl–aminopurine (BAP) + 0.1 mg L−1 indole–3–butyric acid (IBA). After the second subculture, the number of new adventitious shoots was 5.18 ± 0.18 and 4.95 ± 0.18 shoots per explant, respectively. The plants obtained from the hybrid embryos in a soil mixture soil: peat: sand (3:1:1) were adapted. The main morphological and anatomical features of the leaf blades in newly originated peach hybrids have been studied: the thickness of their tissues and the distribution of stomatal apparatus, as well as the physiological parameters of the photosystem II activity in regenerants cultured in vitro and during their in vivo acclimatization. The high capacity to post aseptic adaptation in the obtained hybrids has been shown.
Fighting COVID-19 Contagion among University Students of Healthcare Professions: An Italian Cross-Sectional Study
Authors: Tofani Marco , Berardi Anna , Marceca Maurizio , Valente Donatella , Mazzaccara Alfonso , Polimeni Antonella , Galeoto Giovanni
Published: December 2021

During the pandemic, most governments around the world temporarily closed educational institutions to contain the spread of the Coronavirus Disease 2019 (COVID-19). The objective of the present study is to evaluate the efficacy of an e-learning course on COVID-19 transmission for healthcare university students, in order to advance the preparedness of healthcare university students against contracting COVID-19 within the general university population. The e-learning course was run using a free web service for education. Access to the course was limited to participants enrolled in degree courses related to healthcare professions within the Italian university system. A specific and validated questionnaire was administered at two different times (pre-test and post-test). A paired sample t-test was then used to evaluate their knowledge on COVID-19. Furthermore, a questionnaire measuring their satisfaction was distributed. Data were analyzed from a qualitative point of view. The course was made available from March to July 2020. Over 25,000 students from different Italian universities and various backgrounds participated in the course. The analysis of final test scores revealed that approximately 97% of participants acquired new knowledge and skills on COVID-19, with a statistically significant improvement (p < 0.05). Therefore, it is possible to state that most students enrolled in degrees relating to healthcare at Italian universities are adequately trained with respect to COVID-19 knowledge. Furthermore, students declared a high satisfaction rate both with the course content, and with the management of the telematic platform used.
COVID-19 Prevention Measures Implemented by Tennis Coaches: The Role of Continent, Experience, and Type of Facility
Authors: Martínez-Gallego Rafael , Fuentes-García Juan Pedro , Crespo Miguel
Published: December 2021

The prevention strategies used by tennis coaches when delivering tennis lessons during the COVID-19 pandemic were analyzed in this study. An ad hoc questionnaire collected data from 655 Spanish and Portuguese speaking tennis coaches working in Latin America and Europe. Differences in the prevention measures were analyzed according to the continent, the coaches’ experience, and the type of facility they worked in. Results showed that coaches used information provided from local and national organizations more than from international ones. Hand hygiene, communication of preventive strategies, and changes in the coaching methodology were the most used prevention measures. Latin American coaches and those working in public facilities implemented the measures more often than their European colleagues or those working in private venues. Finally, more experienced coaches showed a greater awareness of the adoption of the measures than their less experienced counterparts. The data provided by this research may assist in developing new specific guidelines, protocols, and interventions to help better understand the daily delivery of tennis coaching in this challenging context.
Adopting Artificial Intelligence in Public Healthcare: The Effect of Social Power and Learning Algorithms
Authors: Sun Tara Qian
Published: December 2021

Although the use of artificial intelligence (AI) in healthcare is still in its early stages, it is important to understand the factors influencing its adoption. Using a qualitative multi-case study of three hospitals in China, we explored the research of factors affecting AI adoption from a social power perspective with consideration of the learning algorithm abilities of AI systems. Data were collected through semi-structured interviews, participative observations, and document analysis, and analyzed using NVivo 11. We classified six social powers into knowledge-based and non-knowledge-based power structures, revealing a social power pattern related to the learning algorithm ability of AI.
Pet Ownership and Multiple Sclerosis during COVID-19
Authors: Oliver-Hall Holly , Ratschen Elena , Tench Christopher R. , Brooks Helen , Constantinescu Cris S. , Edwards Laura
Published: December 2021

Background: Multiple sclerosis (MS) is associated with lower quality of life, reduced social participation, and decreased self-efficacy. The COVID-19 pandemic has had documented effects on the health and wellbeing of people with and without MS. Previous research has demonstrated the positive impact pets can have for people living with long-term conditions. Objectives: To explore the rates of pet ownership and pet attachment in people living with MS and pet ownership associations with quality of life, satisfaction with social roles, and self-efficacy scores; and to explore the effects of the COVID-19 outbreak on people’s perceived relationships with their pets. Materials and Methods: A postal questionnaire was distributed to members of a local MS Register and a control group of people without MS. The questionnaire assessed quality of life, satisfaction with social roles, self-efficacy, the perceived roles of pets, and pet-related concerns experienced during the COVID-19 pandemic. Results: No apparent difference in attachment to pets was found between the patient and control groups. Pet ownership and level of attachment were not associated with differences in quality of life or self-efficacy scores in people living with MS. Using multiple regression analysis, pet ownership was associated with a decrease in satisfaction with participation in social roles, but with the estimated effect being small compared to having a diagnosis of MS or being unemployed. Most participants reported that pets had positive roles during the pandemic, and the most reported pet-related concern was access to veterinary treatment. Conclusion: Pet owners both with and without MS reported subjective benefits to their wellbeing from pet ownership during COVID-19, although analysis suggested that pet ownership was associated with a reduction in satisfaction with social roles. The study had several limitations and suggestions are made for future work.
Objective Job Demands of Oneself and One’s Partner, and Depressive Symptoms. Evidence from a Nationally Representative Longitudinal Study
Authors: Kretzler Benedikt , König Hans-Helmut , Hajek André
Published: December 2021

Background: Job characteristics are an important predictor of depressive symptoms. Recent research detected unemployment’s spillover effects on spouses’ depressive symptoms, but there is still a lack of studies that examine the association between objective job demands of oneself and one’s partner and depressive symptoms. Methods: Data were derived from the German Ageing Survey (DEAS), which is a representative sample that includes individuals aged 40 years and older. Psycho-social, physical, and overall job characteristics were assessed objectively, using a validated index developed by Kroll. Depressive symptoms were quantified by the Center for Epidemiologic Studies Depression Scale (CES-D). Results: Regarding fixed-effects regression, we found no significant association between the own or the partner’s job demands and depression among the total sample and among men. However, among women, both increasing psychosocial demands of one’s own occupation and physical job demands of one’s partner’s occupation were related to higher levels of depression, as well as the partner’s overall job demands. Conclusions: The findings of the present longitudinal study highlight the association between job demands and depressive symptoms in women, but not in men, especially regarding the partner’s employment characteristics. Efforts to reduce the burden of high job demands may be helpful. This could help alleviate depressive symptoms. In turn, geriatric giants caused by increased depressive symptoms, such as frailty, could be postponed.
Sustainable Program Assessment Practices: A Review of the ABET and NCAAA Computer Information Systems Accreditation Process
Authors: Saeed Saqib , Almuhaideb Abdullah M. , Bamarouf Yasser A. , Alabaad Dina A. , Gull Hina , Saqib Madeeha , Iqbal Sardar Zafar , Salam Asiya Abdus
Published: December 2021

Program outcome assessment is a complex process that demands careful planning and resources in order to accurately assess higher-order thinking skills. A well-defined assessment approach provides detailed insights into program weaknesses and leads to continuous improvement. Whereas a poor assessment approach does not reflect the underlying weaknesses and may result in a useless effort. Furthermore, each accreditation body may have a different recommended outcome measurement approach. As a result, academic institutions may make adhoc choices just to satisfy accreditation requirements rather than designing a sustainable measurement approach. On the other hand, the magnitude of huge tasks for satisfying multiple accreditation bodies results in fatigue and mental stress for academic staff. ABET is a well-known international program accreditation body, and NCAAA is a local accreditation body for academic programs in the Kingdom of Saudi Arabia. In this paper, we have documented that how a sustainable outcome measurement mechanism can be designed to satisfy both ABET and NCAAA requirements. The core contribution of this paper is relevant specifically for academic programs in the Kingdom striving to meet both ABET and NCAAA requirements and is also relevant for all education programs to design an appropriate program assessment approach to ensure a sustainable process to foster better learning among students.
Acceptance of COVID-19 Vaccination among Healthcare and Non-Healthcare Workers of Hospitals and Outpatient Clinics in the Northern Region of Slovakia
Authors: Ulbrichtova Romana , Svihrova Viera , Tatarkova Maria , Hudeckova Henrieta , Svihra Jan
Published: December 2021

The purpose of this study was to analyse attitudes, motivation, and reasons for hesitancy toward COVID-19 vaccination among healthcare workers (HCWs) in northern Slovakia. A cross-sectional study was conducted between 30 August 2021 and 30 September 2021. An anonymous questionnaire was administered. The study was completed by 1277 employees. Multivariate logistic regression was used to identify predictors of COVID-19 vaccination status. A total of 1076 (84.3%) were vaccinated, and 201 (15.7%) were unvaccinated. Physician job type (OR = 1.77; CI95 1.13–2.78), history of COVID-19 (OR = 0.37; CI95 0.26–0.37), influenza vaccination at any time (OR = 1.97; CI95 1.12–3.46), compulsory vaccination for HCWs (OR = 9.15; CI95 2.92–28.62), and compulsory vaccination for selected groups (OR = 9.71; CI95 2.75–34.31) were the predictors significantly associated with COVID-19 vaccination acceptance. Non-physician HCWs, employees in hospitals, and employees without a history of COVID-19 significantly more distrusted the efficacy of vaccines against COVID-19. Results of our study confirmed that physicians have higher vaccination rates and lower hesitance to get vaccinated than non-physician HCWs. HCWs play an important role in influencing vaccination decisions and can be helpful in vaccine advocacy to the general public.
Relationship between IgA Nephropathy and Porphyromonas gingivalis; Red Complex of Periodontopathic Bacterial Species
Authors: Nagasawa Yasuyuki , Nomura Ryota , Misaki Taro , Ito Seigo , Naka Shuhei , Wato Kaoruko , Okunaka Mieko , Watabe Maiko , Fushimi Katsuya , Tsuzuki Kenzo , Matsumoto-Nakano Michiyo , Nakano Kazuhiko
Published: December 2021

IgA nephropathy (IgAN) has been considered to have a relationship with infection in the tonsil, because IgAN patients often manifest macro hematuria just after tonsillitis. In terms of oral-area infection, the red complex of periodontal bacteria (Porphyromonas gingivalis (P. gingivalis), Treponema denticol (T. denticola) and Tannerella forsythia (T. forsythia)) is important, but the relationship between these bacteria and IgAN remains unknown. In this study, the prevalence of the red complex of periodontal bacteria in tonsil was compared between IgAN and tonsillitis patients. The pathogenicity of IgAN induced by P. gingivalis was confirmed by the mice model treated with this bacterium. The prevalence of P. gingivalis and T. forsythia in IgAN patients was significantly higher than that in tonsillitis patients (p < 0.001 and p < 0.05, respectively). A total of 92% of tonsillitis patients were free from red complex bacteria, while only 48% of IgAN patients had any of these bacteria. Nasal administration of P. gingivalis in mice caused mesangial proliferation (p < 0.05 at days 28a nd 42; p < 0.01 at days 14 and 56) and IgA deposition (p < 0.001 at day 42 and 56 after administration). Scanning-electron-microscopic observation revealed that a high-density Electron-Dense Deposit was widely distributed in the mesangial region in the mice kidneys treated with P. gingivalis. These findings suggest that P. gingivalis is involved in the pathogenesis of IgAN.
PCSK9 Imperceptibly Affects Chemokine Receptor Expression In Vitro and In Vivo
Authors: Sundararaman Sai Sahana , Peters Linsey J. F. , Nazir Sumra , Marquez Andrea Bonnin , Bouma Janneke E. , Bayasgalan Soyolmaa , Döring Yvonne , van der Vorst Emiel P. C.
Published: December 2021

Proprotein convertase subtilin/kexin type 9 (PCSK9) is a protease secreted mainly by hepatocytes and in lesser quantities by intestines, pancreas, and vascular cells. Over the years, this protease has gained importance in the field of cardiovascular biology due to its regulatory action on the low-density lipoprotein receptor (LDLR). However, recently, it has also been shown that PCSK9 acts independent of LDLR to cause vascular inflammation and increase the severity of several cardiovascular disorders. We hypothesized that PCSK9 affects the expression of chemokine receptors, major mediators of inflammation, to influence cardiovascular health. However, using overexpression of PCSK9 in murine models in vivo and PCSK9 stimulation of myeloid and vascular cells in vitro did not reveal influences of PCSK9 on the expression of certain chemokine receptors that are known to be involved in the development and progression of atherosclerosis and vascular inflammation. Hence, we conclude that the inflammatory effects of PCSK9 are not associated with the here investigated chemokine receptors and additional research is required to elucidate which mechanisms mediate PCSK9 effects independent of LDLR.
Zfhx3 Transcription Factor Represses the Expression of SCN5A Gene and Decreases Sodium Current Density (INa)
Authors: Rubio-Alarcón Marcos , Cámara-Checa Anabel , Dago María , Crespo-García Teresa , Nieto-Marín Paloma , Marín María , Merino José Luis , Toquero Jorge , Salguero-Bodes Rafael , Tamargo Juan , Cebrián Jorge , Delpón Eva , Caballero Ricardo
Published: December 2021

The ZFHX3 and SCN5A genes encode the zinc finger homeobox 3 (Zfhx3) transcription factor (TF) and the human cardiac Na+ channel (Nav1.5), respectively. The effects of Zfhx3 on the expression of the Nav1.5 channel, and in cardiac excitability, are currently unknown. Additionally, we identified three Zfhx3 variants in probands diagnosed with familial atrial fibrillation (p.M1260T) and Brugada Syndrome (p.V949I and p.Q2564R). Here, we analyzed the effects of native (WT) and mutated Zfhx3 on Na+ current (INa) recorded in HL-1 cardiomyocytes. ZFHX3 mRNA can be detected in human atrial and ventricular samples. In HL-1 cardiomyocytes, transfection of Zfhx3 strongly reduced peak INa density, while the silencing of endogenous expression augmented it (from −65.9 ± 8.9 to −104.6 ± 10.8 pA/pF; n ≥ 8, p < 0.05). Zfhx3 significantly reduced the transcriptional activity of human SCN5A, PITX2, TBX5, and NKX25 minimal promoters. Consequently, the mRNA and/or protein expression levels of Nav1.5 and Tbx5 were diminished (n ≥ 6, p < 0.05). Zfhx3 also increased the expression of Nedd4-2 ubiquitin-protein ligase, enhancing Nav1.5 proteasomal degradation. p.V949I, p.M1260T, and p.Q2564R Zfhx3 produced similar effects on INa density and time- and voltage-dependent properties in WT. WT Zfhx3 inhibits INa as a result of a direct repressor effect on the SCN5A promoter, the modulation of Tbx5 increasing on the INa, and the increased expression of Nedd4-2. We propose that this TF participates in the control of cardiac excitability in human adult cardiac tissue.
Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines
Authors: Lu Wei , Wang Ruolin , Wang Pan , Ma Sanyuan , Xia Qingyou
Published: December 2021

Inducible gene expression systems are important tools for studying gene function and to control protein synthesis. With the completion of the detailed map of the silkworm (Bombyx mori) genome, the study of Bombyx mori has entered the post-genome era. While the functions of many genes have been described in detail, many coding genes remain unidentified. Except for the available tetracycline induction system, there is currently a dearth of other effective induction systems for B. mori. A genetic code expansion system can be used for protein labeling and to regulate gene expression. Here, we have established a genetic code expansion system for B. mori based on the well-researched tRNAPyl/PylRS pair from Methanosarcina mazei. We used H-Lys(Boc)-OH, which is a lysine derivative to efficiently and tightly control the expression of the reporter gene DsRed[TAG]EGFP (D[TAG]G), which encoded a H-Lys(Boc)-OH-bearing protein fused with DsRed and EGFP (here regarded as D[Boc]G) in B. mori cell lines BmE and BmNs. In D[TAG]G, the amber stop codon is recognized as the orthogonal tRNAPyl. Successful application of genetic code expansion system in silkworm cell lines will support the research into the function of silkworm genes and paves the way for the identification of new genes and protein markers in silkworm.
A Review of Robotic-Assisted Bronchoscopy Platforms in the Sampling of Peripheral Pulmonary Lesions
Authors: Lu Michael , Nath Sridesh , Semaan Roy W.
Published: December 2021

Robotic-assisted bronchoscopy is one of the newest additions to clinicians’ armamentarium for the biopsy of peripheral pulmonary lesions in light of the suboptimal yields and sensitivities of conventional bronchoscopic platforms. In this article, we review the existing literature pertaining to the feasibility as well as sensitivity of available robotic-assisted bronchoscopic platforms.
Post-Contrast Acute Kidney Injury after Acute Stroke—Insights from a German Tertiary Care Center
Authors: Frank Benedikt , Escolà Jordi Kühne , Biermann-Ratjen Leoni , Hüsing Anika , Li Yan , Dammann Philipp , Sure Ulrich , Kleinschnitz Christoph , Forsting Michael , Köhrmann Martin , Deuschl Cornelius
Published: December 2021

Background: Our aim was to investigate the relationship between additional iodinated contrast medium (CM) application for acute stroke imaging and Post-Contrast Acute Kidney Injury (PC-AKI). Methods: We performed a retrospective analysis of consecutive patients with acute stroke who received a CT angiogram (CTA) with or without additional CT perfusion (CTP) at admission between 2017 and 2020. The primary endpoint was the incidence of PC-AKI. Potential causes of renal function impairment were recorded and logistic regression was performed to determine predictors of PC-AKI. Results: Of 3134 cases screened, n = 989 met the predefined inclusion criteria. PC-AKI occurred in 22 (5.4%) patients who received CTA only and 18 (3.1%) patients who received CTA and additional CTP (unadjusted OR, CI; 0.59, 0.29–1.05). In 31/40 (77.5%) patients who suffered PC-AKI, a non-CM-related cause of renal function impairment was identified. Stroke etiology (hemorrhagic vs. ischemic) and indicators of prior kidney disease were independent predictors of PC-AKI. Conclusions: Additional administration of CM for perfusion imaging in acute stroke did not show a relevant influence on the occurrence of PC-AKI. Patients with intracranial hemorrhage and/or prior kidney disease are at particular risk of developing AKI.