bluuebunny/crossref_metadata_2025 huggingface dataset:
Heat Production Capacity Simulation and Parameter Sensitivity Analysis in the Process of Thermal Reservoir Development
Authors: Yang Yi , Fu Guoqiang , Zhao Jingtao , Gu Lei
Published: October 2023

The development of a geothermal system involves changes in the temperature field (T), seepage field (H), stress field (M), and chemical field (C) and the influence among them and injecting the heat extraction working fluid into the injection well that flows (migrating) through natural fractures and exchanges heat with the geothermal high-temperature rock. At the same time, the injection of low-temperature working fluid will induce thermal stress, resulting in changes in the reservoir temperature field and stress field. To study the influence factors and influence degree of heat production performance and mining life under multi-field coupling in the process of thermal reservoir development, based on THMC multi-field coupling numerical simulation software, this paper deeply studies the control differential equations and boundary coupling conditions of rock mass (fracture) deformation, seepage, heat exchange, the chemical reaction, and other processes based on the numerical solution method of the discrete fracture network model, simulating heat production capacity during the deep geothermal resource extraction process. The reservoir geological model analysis and generalization, parameter setting, boundary conditions, initial condition settings, mesh generation, and other steps were carried out in turn. Two different heat extraction working fluids, water, and CO2 were selected for numerical simulation in the mining process. The changes in the thermal reservoir temperature, net heat extraction rate, and SiO2 concentration during the thirty years of systematic mining were compared. The results show that CO2 has a better heat extraction effect. Finally, the reservoir thermal conductivity, heat capacity, well spacing, injection temperature, fracture spacing, fracture permeability, fracture number, fracture length, and other parameters were set, respectively. The parameter variation range was set, and the parameter sensitivity analysis was carried out. The numerical simulation results show that the engineering production conditions (injection temperature, well spacing) have little effect on the thermal efficiency and mining life, and the properties of fractures (fracture permeability, fracture number, fracture length) have a great influence.
Optimal Probabilistic Allocation of Photovoltaic Distributed Generation: Proposing a Scenario-Based Stochastic Programming Model
Authors: Kheirkhah Ali Reza , Meschini Almeida Carlos Frederico , Kagan Nelson , Leite Jonatas Boas
Published: October 2023

The recent developments in the design, planning, and operation of distribution systems indicate the need for a modern integrated infrastructure in which participants are managed through the perceptions of a utility company in an economic network (e.g., energy loss reduction, restoration, etc.). The penetration of distributed generation units in power systems are growing due to their significant influence on the key attributes of power systems. As a result, the placement, type, and size of distributed generations have an essential role in reducing power loss and lowering costs. Power loss minimization, investment and cost reduction, and voltage profile improvement combine to form a conceivable goal function for distributed generation allocation in a constrained optimization problem, and they require a complex procedure to control them in the most appropriate way while satisfying network constraints. Such a complex decision-making procedure can be solved by adjusting the dynamic optimal power flow problem to the associated network. The purpose of the present work is to handle the distributed generation allocation problem for photovoltaic units, attempting to reduce energy and investment costs while accounting for generation unpredictability as well as load fluctuation. The problem is analyzed under various scenarios of solar radiation through a stochastic programming technique because of the intense uncertainty of solar energy resources. The formulation of photovoltaic distributed generation allocation is represented as a mixed-integer second-order conic programming problem. The IEEE 33-bus and real-world 136-bus distribution systems are tested. The findings illustrate the efficacy of the proposed mathematical model and the role of appropriate distributed generation allocation.
Improved Delayed Detached Eddy Simulation of Combustion of Hydrogen Jets in a High-Speed Confined Hot Air Cross Flow II: New Results
Authors: Bakhne Sergei , Vlasenko Vladimir , Troshin Alexei , Sabelnikov Vladimir , Savelyev Andrey
Published: October 2023

The improved delayed detached Eddy simulation (IDDES) approach used in the part I of this investigation to study the self-ignition and combustion of hydrogen jets in a high-speed transverse flow of hot vitiated air in a duct is extended in the following directions: (i) the wall boundary conditions are modified to take into account the optical windows employed in the experiments; (ii) the detailed chemical kinetic model with 19 reactions is used; (iii) a nonlinear turbulence model is implemented in the code to capture the secondary flows in the duct corners; (iv) the wall roughness model is adapted; (v) the synthetic turbulence generator is imposed upstream of the fuel injection. As a result of improving the mathematical and physical problem statements, a good agreement between the simulation and the experimental database obtained at the LAERTE workbench (ONERA) is achieved.
Battery Fault Diagnosis Method Based on Online Least Squares Support Vector Machine
Authors: Zhang Tongrui , Li Ran , Zhou Yongqin
Published: October 2023

Battery fault diagnosis technology is crucial for the reliable functioning of battery systems. This research introduces an online least squares support vector machine method tailored for battery fault diagnosis. After examining battery fault types and gathering relevant data, this method creates a diagnostic model, effectively addressing small and sporadic fault data that is inadequately handled by conventional support vector machines. Recognizing that certain battery malfunctions evolve over time and are multifaceted, confidence intervals have been integrated into the diagnostic models, enhancing accuracy. Upon testing this model using empirical data, it demonstrated rapid diagnostic capabilities and outperformed other algorithms in identifying progressive faults, ensuring precise fault identification, minimizing false alarms, and bolstering battery system safety.
Development of Functional Gluten-Free Sourdough Bread with Pseudocereals and Enriched with Moringa oleifera
Authors: Peñalver Rocío , Ros Gaspar , Nieto Gema
Published: October 2023

Celiac patients tend to have an unbalanced diet, because gluten-free products typically contain a high amount of fats and carbohydrates and a low amount of proteins, minerals, and dietary fiber. This research focused on the development of gluten-free functional breads using pseudocereals, psyllium, and gluten-free sourdough to replace commercial yeast, fortifying them with Moringa oleifera. Six different gluten-free breads were made with sourdough: three control breads differentiated by sourdough (quinoa, amaranth, and brown rice) and three breads enriched with moringa leaf differentiated by sourdough. The antioxidant capacity, phenolic compounds, nutritional composition, physicochemical parameters (color, pH, and acidity), folate content, amino acid profile, reducing sugars, mineral composition, mineral bioaccessibility, fatty acid profile, and sensory acceptability were evaluated. A commercial gluten-free (COM) bread was included in these analyses. Compared with COM bread, the reformulated breads were found to have better nutritional properties. Moringa leaf increased the nutritional properties of bread, and highlighted the QM (quinoa/moringa) bread as having increased protein, fiber, sucrose, glucose, maltose, phenylalanine, and cysteine. The AM (amaranth/moringa) bread was also shown to have a higher total folate content, antioxidant capacity, phenolic compounds, 9t,11t-C18:2 (CLA), and 9t-C18:1. Reformulated breads enriched with moringa could meet nutritional requirements and provide health benefits to people with celiac disease.
Raman Spectroscopic and Sensory Evaluation of Cocoa Liquor Prepared with Ecuadorian Cocoa Beans Treated with Gamma Irradiation or Induced Electromagnetic Field Fermentation
Authors: Guzmán-Armenteros Tania María , Ruales Jenny , Cuesta-Plúa Cristina , Bravo Juan , Sinche Marco , Vera Edwin , Vera Edison , Vargas-Jentzsch Paul , Ciobotă Valerian , Ortega-Ojeda Fernando E. , Proaño Andrés , Echeverría Armando , Ramos-Guerrero Luis
Published: October 2023

Cocoa liquor is the primary precursor of the worldwide highly appreciated commodity chocolate. Its quality depends on several factors, such as the type of cocoa, the fermentation process, and the control of the contaminants in the fermented beans. This study aims to evaluate whether the induced magnetic field treatment during the fermentation process or the pathogen reduction with gamma irradiation after the fermentation affect the characteristics of the cocoa liquor obtained from Ecuadorian cocoa beans. For this purpose, liquor samples from controls (standard process), from beans treated with an induced magnetic field up to 80 mT, and from beans irradiated with nominal doses up to 3 kGy were characterized through Raman spectroscopic analysis and sensorial evaluation. The most relevant bands of the cocoa liquor were assigned according to reports from the literature, spectroscopic data, and chemometrics. The spectra corresponding to different treatments and doses were visually very similar, but they could be discriminated using OPLS-DA models, where the most intense Raman signals were attributed to the lipid components. The sensorial evaluation rated the presence of floral, fruity, almondy, acid, and bitter flavors, along with astringency and intense aroma, and these attributes exhibited variable behavior depending on the dose of the irradiation or magnetic treatment. Therefore, both treatments may exert an influence on cocoa beans and, therefore, on the cocoa liquor quality.
Evolution Law of Shallow Water in Multi-Face Mining Based on Partition Characteristics of Catastrophe Theory
Authors: Zhang Yujiang , Cui Bingyuan , Wang Yining , Zhang Shuai , Feng Guorui , Zhang Zhengjun
Published: October 2023

It is of great significance for ecological environment protection to clarify the regional evolution characteristics of shallow water under the disturbance of multi-working face mining. In this paper, the catastrophe theory method, GIS spatial analysis function and FEFLOW numerical calculation method were comprehensively used to study the instability risk and evolution law of shallow water systems in the Zhuan Longwan Coal Mine. The results show that: the Zhuan Longwan Coal Mine is divided into five areas (small risk area, light risk area, middle risk area, heavy risk area and special risk area) based on catastrophe theory, among which the middle risk area has the largest area of 16,616,880 m2, and the special risk area has the smallest area of 1,769,488 m2. Based on the results of catastrophe zoning, the evolution law of shallow water under multi-surface disturbance in different zones is expounded. In the middle-risk area, the water level drop at measuring point 4 is the largest, which is 0.525 m, and the water level drop at measuring point 5 is the smallest, which is 0.116 m. The study aims to provide a basis for regional coal development planning and research on the method of water-retaining coal mining.
Using Diffusion of Innovation Theory to Address Health of the Homeless in Hawaii
Authors: Spencer Lisa , Tanner Anita , Lu Kristina
Published: October 2023

Policy diffusion is the process in which an innovation is communicated over time through various channels among the members of a social system. It is a special type of communication, in that the messages are concerned with new ideas. Diffusion theory or diffusion of innovations is the theoretical framework utilized in this study to compare successful programs aimed at providing health care for the homeless population. This study examines the Crisis Outreach Response and Engagement (CORE) program in Hawaii and explains how the diffusion innovation theory and programs in other states can be used to develop policies and processes to successfully address the medical and crisis intervention needs of the homeless on O’ahu. The study also includes suggested recommendations and strategies for the CORE program.
Professional Narratives about Older Adults and Health Services Responsive to Fall-Inducing Frailty
Authors: Xavier Laudicéia Noronha , do Nascimento Vânia Barbosa
Published: October 2023

The second external cause of death from unintentional injuries is falls in people over 60 and is a worldwide Public Health problem. Associated factors are identified early in Primary Health Care. Thus, we analyze professional narratives about older adults/old age and the organization of services in the presence of fall-inducing frailty. A structured narrative was applied under the following stages: understanding the context, setting/plot/character analysis, and interpretive synthesis. Data were collected from August to November 2022, distributing 21 health professionals in three Narrative Focus Groups. In the analyses, the collective conceptions dialogued with Bourdieu’s Epistemology of field, habitus, and capital. Technical and common sense representations of older adults were simultaneously observed among the results, along with the belief of old age as a problematic life stage. Care is centered on the installed disease/ailment. Encouraging autonomy and self-care emerges in integrative health practices, which older adults underestimate. Professionals access the lives of older adults according to their habitus, which, in turn, is structured (structuring) in the disputes for installed capital. Thus, the care provided disregards subjectivities and symbolic systems associated with falls.
Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase
Authors: Canales Javier , Verdejo José F. , Calderini Daniel F.
Published: October 2023

Climate-change-induced temperature fluctuations pose a significant threat to crop production, particularly in the Southern Hemisphere. This study investigates the transcriptome and physiological responses of rapeseed to post-flowering temperature increases, providing valuable insights into the molecular mechanisms underlying rapeseed tolerance to heat stress. Two rapeseed genotypes, Lumen and Solar, were assessed under control and heat stress conditions in field experiments conducted in Valdivia, Chile. Results showed that seed yield and seed number were negatively affected by heat stress, with genotype-specific responses. Lumen exhibited an average of 9.3% seed yield reduction, whereas Solar showed a 28.7% reduction. RNA-seq analysis of siliques and seeds revealed tissue-specific responses to heat stress, with siliques being more sensitive to temperature stress. Hierarchical clustering analysis identified distinct gene clusters reflecting different aspects of heat stress adaptation in siliques, with a role for protein folding in maintaining silique development and seed quality under high-temperature conditions. In seeds, three distinct patterns of heat-responsive gene expression were observed, with genes involved in protein folding and response to heat showing genotype-specific expression. Gene coexpression network analysis revealed major modules for rapeseed yield and quality, as well as the trade-off between seed number and seed weight. Overall, this study contributes to understanding the molecular mechanisms underlying rapeseed tolerance to heat stress and can inform crop improvement strategies targeting yield optimization under changing environmental conditions.
Encapsulation in Oxygen-Loaded Nanobubbles Enhances the Antimicrobial Effectiveness of Photoactivated Curcumin
Authors: Munir Zunaira , Molinar Chiara , Banche Giuliana , Argenziano Monica , Magnano Greta , Cavallo Lorenza , Mandras Narcisa , Cavalli Roberta , Guiot Caterina
Published: October 2023

In both healthcare and agriculture, antibiotic resistance is an alarming issue. Biocompatible and biodegradable ingredients (e.g., curcumin) are given priority in “green” criteria supported by the Next Generation EU platform. The solubility and stability of curcumin would be significantly improved if it were enclosed in nanobubbles (NB), and photoactivation with the correct wavelength of light can increase its antibacterial efficacy. A continuous release of curcumin over a prolonged period was provided by using innovative chitosan-shelled carriers, i.e., curcumin-containing nanobubbles (Curc-CS-NBs) and oxygen-loaded curcumin-containing nanobubbles (Curc-Oxy-CS-NBs). The results demonstrated that after photoactivation, both types of NBs exhibited increased effectiveness. For Staphylococcus aureus, the minimum inhibitory concentration (MIC) for Curc-CS-NBs remained at 46 µg/mL following photodynamic activation, whereas it drastically dropped to 12 µg/mL for Curc-Oxy-CS-NBs. Enterococcus faecalis shows a decreased MIC for Curc-CS-NB and Curc-Oxy-CS-NB (23 and 46 µg/mL, respectively). All bacterial strains were more effectively killed by NBs that had both oxygen and LED irradiation. A combination of Curc-Oxy-CS-NB and photodynamic stimulation led to a killing of microorganisms due to ROS-induced bacterial membrane leakage. This approach was particularly effective against Escherichia coli. In conclusion, this work shows that Curc-CS-NBs and Curc-Oxy-CS-exhibit extremely powerful antibacterial properties and represent a potential strategy to prevent antibiotic resistance and encourage the use of eco-friendly substitutes in agriculture and healthcare.
The Dual Luminescence Lifetime pH/Oxygen Sensor: Evaluation of Applicability for Intravital Analysis of 2D- and 3D-Cultivated Human Endometrial Mesenchymal Stromal Cells
Authors: Litvinov Ilia K. , Belyaeva Tatiana N. , Salova Anna V. , Aksenov Nikolay D. , Chelushkin Pavel S. , Solomatina Anastasia I. , Tunik Sergey P. , Kornilova Elena S.
Published: October 2023

The oxygenation of cells and tissues and acidification of the cellular endolysosomal system are among the major factors that ensure normal functioning of an organism and are violated in various pathologies. Recording of these parameters and their changes under various conditions is an important task for both basic research and clinical applications. In the present work, we utilized internalizable dual pH/O2 lifetime sensor (Ir-HSA-FITC) based on the covalent conjugation of human serum albumin (HSA) with fluorescein isothiocyanate (FITC) as pH sensor and an orthometalated iridium complex as O2 sensor. The probe was tested for simultaneous detection of acidification level and oxygen concentration in endolysosomes of endometrial mesenchymal stem/stromal cells (enMSCs) cultivated as 2D monolayers and 3D spheroids. Using a combined FLIM/PLIM approach, we found that due to high autofluorescence of enMSCs FITC lifetime signal in control cells was insufficient to estimate pH changes. However, using flow cytometry and confocal microscopy, we managed to detect the FITC signal response to inhibition of endolysosomal acidification by Bafilomycin A1. The iridium chromophore phosphorescence was detected reliably by all methods used. It was demonstrated that the sensor, accumulated in endolysosomes for 24 h, disappeared from proliferating 2D enMSCs by 72 h, but can still be recorded in non-proliferating spheroids. PLIM showed high sensitivity and responsiveness of iridium chromophore phosphorescence to experimental hypoxia both in 2D and 3D cultures. In spheroids, the phosphorescence signal was detected at a depth of up to 60 μm using PLIM and showed a gradient in the intracellular O2 level towards their center.
Features and Comparative Characteristics of Fucosylated Glycans Expression in Endothelial Glycocalyx of Placental Terminal Villi in Patients with Preeclampsia Treated with Different Antihypertensive Regimens
Authors: Ziganshina Marina M. , Kulikova Galina V. , Muminova Kamilla T. , Shchegolev Alexander I. , Yarotskaya Ekaterina L. , Khodzhaeva Zulfiya S. , Sukhikh Gennady T.
Published: October 2023

Antihypertensive therapy is an essential part of management of patients with preeclampsia (PE). Methyldopa (Dopegyt®) and nifedipine (Cordaflex®) are basic medications of therapy since they stabilize blood pressure without affecting the fetus. Their effect on the endothelium of placental vessels has not yet been studied. In this study, we analyzed the effect of antihypertensive therapy on the expression of fucosylated glycans in fetal capillaries of placental terminal villi in patients with early-onset PE (EOPE) and late-onset PE (LOPE), and determined correlation between their expression and mother’s hemodynamic parameters, fetoplacental system, factors reflecting inflammatory response, and destructive processes in the endothelial glycocalyx (eGC). A total of 76 women were enrolled in the study: the comparison group consisted of 15 women with healthy pregnancy, and the main group comprised 61 women with early-onset and late-onset PE, who received one-component or two-component antihypertensive therapy. Hemodynamic status was assessed by daily blood pressure monitoring, dopplerometry of maternal placental and fetoplacental blood flows, and the levels of IL-18, IL-6, TNFα, galectin-3, endocan-1, syndecan-1, and hyaluronan in the blood of the mother. Expression of fucosylated glycans was assessed by staining placental sections with AAL, UEA-I, LTL lectins, and anti-LeY MAbs. It was found that (i) expression patterns of fucosylated glycans in eGC capillaries of placental terminal villi in EOPE and LOPE are characterized by predominant expression of structures with a type 2 core and have a similar pattern of quantitative changes, which seems to be due to the impact of one-component and two-component antihypertensive therapy on their expression; (ii) correlation patterns indicate interrelated changes in the molecular composition of eGC fucoglycans and indicators reflecting changes in maternal hemodynamics, fetoplacental hemodynamics, and humoral factors associated with eGC damage. The presented study is the first to demonstrate the features of placental eGC in women with PE treated with antihypertensive therapy. This study also considers placental fucoglycans as a functional part of the eGC, which affects hemodynamics in the mother–placenta–fetus system.
In Vitro Imaging and Molecular Characterization of Ca2+ Flux Modulation by Nanosecond Pulsed Electric Fields
Authors: Camera Francesca , Colantoni Eleonora , Garcia-Sanchez Tomas , Benassi Barbara , Consales Claudia , Muscat Adeline , Vallet Leslie , Mir Luis M. , Andre Franck , Merla Caterina
Published: October 2023

In recent years, the application of pulsed electric fields with very short durations (nanoseconds) and extremely high amplitudes (MV/m) has been investigated for novel medical purposes. Various electric protocols have been explored for different objectives, including the utilization of fractionated pulse doses to enhance cell electrosensitization to the uptake of different markers or an increase in apoptosis. This study focused on the use of fluorescence imaging to examine molecular calcium fluxes induced by different fractionated protocols of short electric pulses in neuroblastoma (SH-SY5Y) and mesenchymal stem cells (HaMSCs) that were electroporated using nanosecond pulsed electric fields. In our experimental setup, we did not observe cell electrosensitization in terms of an increase in calcium flux following the administration of fractionated doses of nanosecond pulsed electric fields with respect to the non-fractionated dose. However, we observed the targeted activation of calcium-dependent genes (c-FOS, c-JUN, EGR1, NURR-1, β3-TUBULIN) based on the duration of calcium flux, independent of the instantaneous levels achieved but solely dependent on the final plateau reached. This level of control may have potential applications in various medical and biological treatments that rely on calcium and the delivery of nanosecond pulsed electric fields.
Vimentin Localization in the Zebrafish Oral Cavity: A Potential Role in Taste Buds Regeneration
Authors: Aragona Marialuisa , Porcino Caterina , Briglia Marilena , Mhalhel Kamel , Abbate Francesco , Levanti Maria , Montalbano Giuseppe , Laurà Rosaria , Lauriano Eugenia Rita , Germanà Antonino , Guerrera Maria Cristina
Published: October 2023

The morphology of the oral cavity of fish is related to their feeding habits. In this context, taste buds are studied for their ability to catch chemical stimuli and their cell renewal capacity. Vimentin RV202 is a protein employed as a marker for mesenchymal cells that can differentiate along different lineages and to self-renew, while Calretinin N-18 is employed as a marker of sensory cells, and ubiquitin is a protein crucial for guiding the fate of stem cells throughout development. In this study, a surface morphology investigation and an immunohistochemical analysis have been conducted. The results of the present study reveal, for the first time, the presence of Vimentin RV202 in a taste bud cell population of zebrafish. Some taste bud cells are just Vimentin RV202-immunoreactive, while in other cells Vimentin RV202 and Calretinin N-18 colocalize. Some taste buds are just reactive to Calretinin N-18. Vimentin RV202-immunoreactive cells have been observed in the connective layer and in the basal portion of the taste buds. The immunoreactivity of ubiquitin was restricted to sensory cells. Further studies are needed to elucidate the role of Vimentin RV202 in the maturation of taste bud cells, its potential involvement in the regeneration of these chemosensory organs, and its eventual synergic work with ubiquitin.
Parameters Synthesis of Na-Magadiite Materials for Water Treatment and Removal of Basic Blue-41: Properties and Single-Batch Design Adsorber
Authors: Alanazi Abdulaziz M. , Al Dmour Hmoud , Popoola Saheed A. , Oudghiri Hassani Hicham , Rakass Souad , Al-Faze Rawan , Kooli Fethi
Published: October 2023

Na-magadiite materials were prepared from a gel containing a silica source, sodium hydroxide, and water via hydrothermal treatment at different temperatures (130 °C to 170 °C) and periods of time (1 day to 10 days). In this study, four silica sources were selected (fumed silica, colloidal silica, Ludox HS-40%, and Ludox AS-40%). Variable conditions such as sodium hydroxide and water contents were explored at a specific temperature and reaction time. The obtained materials were characterized by using X-ray diffraction (XRD), thermogravimetry differential thermal analysis TG-DTA, scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX), Fourier Transform Infrared spectroscopy (FTIR), solid 29Si magic angle spinning magnetic nuclear resonance (MAS MNR, and nitrogen adsorption isotherms. A pure Na-magadiite phase was obtained from the four silica sources at a synthesis temperature of 150 °C after a period of one to two days with a characteristic basal spacing of 1.54 nm. At a longer reaction time of 3 days and a higher temperature of 170 °C, Na-kenyaite with a basal spacing of 2.01 nm was achieved, in addition to a quartz phase. The content of water or sodium hydroxide in the gel affected the nature of the prepared phases. A cauliflower-like morphology was obtained from colloidal silica sources, while a different morphology was achieved using solid fumed silica. The 29Si solid NMR confirmed the presence of Q3 and Q4 silicon sites in the Na-magadiite materials. The optimal Na-magadiite materials at 150 °C for 2 days were assessed for their ability to remove Basic Blue-41 dye from artificially contaminated aqueous solution. The Langmuir equation was used to estimate the maximum removal capacity. A maximum removal capacity of 219 mg/g was achieved using Na-magadiite prepared from a Ludox-HS40% silica source, and a maximum removal capacity of 167 mg/g was observed for Na-magadiite prepared from fumed silica. Basic Blue-4’s removal percentage was enhanced at basic pH levels (8 to 10) to a maximum of 95%. These materials could be regenerated for seven cycles of reuse with a reduction of 27 to 40% of the original values. Therefore, Na-magadiite materials are promising and efficient removal agents for the removal of Basic Blue-41 from effluents.
Modelling of Sediment Transport and Deposition in Generating River-Mouth Closure: Oum-Errabia River, Morocco
Authors: Aouiche Ismail , Sedrati Mouncef , Anthony Edward J.
Published: October 2023

River mouths are dynamic systems that can respond rapidly to both fluxes in fluvial water and sediment discharge and marine energy conditions, notably waves. On semi-arid wave-exposed coasts, the morphosedimentary behaviour of river mouths is particularly sensitive to variations in water discharge, which can be significantly influenced by climate variations, in addition to anthropogenic actions such as the construction of dams for water resource needs. In this climatic setting, an increasingly common consequence of decreasing river water discharge is the more or less prolonged closure of river mouths. Most studies have addressed river-mouth closure using analytical, parametric, numerical, or statistical models. The present study uses output from four numerical models to elucidate the hydrodynamic and sedimentary behaviour of the mouth of the Oum-Errabia River (catchment size: 35,000 km2), which debouches on the Atlantic coast of Morocco. The historical evolution of the river mouth and the impact of human interventions, such as the construction of dams, are discussed. The study also briefly discusses the impact of the recent closure of the river outlet, in response to particularly low water discharge, on the marine ecosystem and water quality. The modelling results covering a one-year simulation in this situation of closure indicate a deposition of 427,400 m3 of sediment in front of the mouth of the Oum-Errabia. Ensuring permanent river-mouth opening and tidal flushing and renewal of this river’s estuarine waters will necessitate costly regular dredging.
Prediction of Sea Level in the Arabian Gulf Using Artificial Neural Networks
Authors: Alenezi Nasser , Alsulaili Abdalrahman , Alkhalidi Mohamad
Published: October 2023

Creating an efficient model for predicting sea level fluctuations is essential for climate change research. This study examined the effectiveness of utilizing Artificial Neural Networks (ANNs), particularly the recurrent network approach. ANNs were chosen for their capacity to learn from extensive and intricate data and their ability to handle nonlinear correlations. The Long Short-Term Memory (LSTM) algorithm was employed to fill data gaps and predict future sea level records in the Arabian Gulf, especially in Mina Salman. The results were promising, with LSTM successfully filling a 6-year data gap while maintaining low Root Mean Square Error (RMSE) and Mean Absolute Percentage Error (MAPE) values. The first phase of the model yielded a RMSE value of 63.4 mm and a MAPE value of 3.14%. The same approach was used to retrain the model with a mix of real and predicted values, preserving historical patterns and yearly rates with an RMSE of 66.5 mm and a MAPE of 3.07%. These findings highlight LSTM’s advantages when considering only historical information for predicting the future sea level changes. The research provides valuable insights into predicting sea level changes in regions with limited field data, such as the Arabian Gulf, and emphasizes the potential for further research to enhance sea level prediction models through improved optimization techniques.
Co-Culture of White Rot Fungi Pleurotus ostreatus P5 and Bacillus amyloliquefaciens B2: A Strategy to Enhance Lipopeptide Production and Suppress of Fusarium Wilt of Cucumber
Authors: Xu Man , Shi Ying , Fan De-Ling , Kang Yi-Jin , Yan Xin-Li , Wang Hong-Wei
Published: October 2023

Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (FOC), poses a serious threat to cucumber productivity. Compared to traditional chemical pesticides, biological control strategies have attracted more attention recently owing to their effectiveness against pathogens and their environmental safety. This study investigated the effect of white rot fungi Pleurotus ostreatus P5 on the production of cyclic lipopeptides (CLPs) of Bacillus amyloliquefaciens B2 and the potential co-culture filtrate of strains B2 and P5 to control cucumber Fusarium wilt. A PCR amplification of CLP genes revealed that B. amyloliquefaciens B2 had two antibiotic biosynthesis genes, namely, ituA and srf, which are involved in iturin A and surfactin synthesis. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) revealed that CLPs derived from strain B2 contained two families, iturin A (C14, C15) and surfactin (C12–C17). The co-culture exhibited an enhanced accumulation of iturin A and surfactin compared to the monoculture of strain B2. Furthermore, the gene expressions of ituA and srf were both significantly upregulated when co-cultured with the fungus compared to monocultures. In an in vitro experiment, the co-culture filtrate and monoculture filtrate of B. amyloliquefaciens B2 inhibited mycelial growth by 48.2% and 33.2%, respectively. In a greenhouse experiment, the co-culture filtrate was superior to the monoculture filtrate in controlling cucumber Fusarium wilt disease and in the promotion of plant growth. Co-culture filtrate treatment significantly enhanced the microbial metabolic activity and decreased the abundance of FOC in the rhizosphere soil. These results show that the co-culture of P. ostreatus P5 and B. amyloliquefaciens B2 has great potential in cucumber Fusarium wilt disease prevention by enhancing the production of bacterial CLPs.
Estimation Technique for IGBT Module Junction Temperature in a High-Power Density Inverter
Authors: Okilly Ahmed H. , Choi Seungdeog , Kwak Sangshin , Kim Namhun , Lee Jonghyuk , Moon Hyoungjun , Baek Jeihoon
Published: October 2023

During the last few decades, insulated-gate bipolar transistor (IGBT) power modules have evolved as reliable and useful electronic parts due to the increasing relevance of power inverters in power infrastructure, reliability enhancement, and long-life operation. Excessive temperature stresses caused by excessive power losses frequently cause high-power-density IGBT modules to fail. As a result, module temperature monitoring techniques are critical in designing and selecting IGBT modules for high-power-density applications to guarantee that temperature stresses in the various module components remain within the rated values. In this paper, a module’s different losses were estimated, a heating pipe system for the thermal power cycling technique was proposed, and finite element method (FEM) thermal modeling and module temperature measurement were performed using ANSYS Icepak software version 2022 R1 to determine whether the IGBT module’s temperature rise was within acceptable bounds. To test the proposed technique, a proposed design structure of the practical railway application with a 3.3 MW traction inverter is introduced using commercialized IGBT modules from Semikron company with maximum temperature of about 150 °C. the FEM analysis results showed that the maximum junction temperature is about 109 °C which is in acceptable ranges, confirming the appropriate selection of the employed IGBT module for the target application.
On the Iterative Methods for the Solution of Three Types of Nonlinear Matrix Equations
Authors: Ivanov Ivan G. , Yang Hongli
Published: October 2023

In this paper, we investigate the iterative methods for the solution of different types of nonlinear matrix equations. More specifically, we consider iterative methods for the minimal nonnegative solution of a set of Riccati equations, a nonnegative solution of a quadratic matrix equation, and the maximal positive definite solution of the equation X+A∗X−1A=Q. We study the recent iterative methods for computing the solution to the above specific type of equations and propose more effective modifications of these iterative methods. In addition, we make comments and comparisons of the existing methods and show the effectiveness of our methods by illustration examples.
Discovery and Anti-Inflammatory Activity of a Cyanobacterial Fatty Acid Targeting the Keap1/Nrf2 Pathway
Authors: Al-Awadhi Fatma H. , Simon Emily F. , Liu Na , Ratnayake Ranjala , Paul Valerie J. , Luesch Hendrik
Published: October 2023

The monounsaturated fatty acid 7(E)-9-keto-hexadec-7-enoic acid (1) and three structurally related analogues with different oxidation states and degrees of unsaturation (2–4) were discovered from a marine benthic cyanobacterial mat collected from Delta Shoal, Florida Keys. Their structures were elucidated using NMR spectroscopy and mass spectrometry. The structure of 1 contained an α,β-unsaturated carbonyl system, a key motif required for the activation of the Keap1/Nrf2−ARE pathway that is involved in the activation of antioxidant and phase II detoxification enzymes. Compounds 1–4 were screened in ARE-luciferase reporter gene assay using stably transfected HEK293 cells, and only 1 significantly induced Nrf2 activity at 32 and 10 µM, whereas 2–4 were inactive. As there is crosstalk between inflammation and oxidative stress, subsequent biological studies were focused on 1 to investigate its anti-inflammatory potential. Compound 1 induced Nqo1, a well-known target gene of Nrf2, and suppressed iNos transcript levels, which translated into reduced levels of nitric oxide in LPS-activated mouse macrophage RAW264.7 cells, a more relevant model for inflammation. RNA sequencing was performed to capture the effects of 1 on a global level and identified additional canonical pathways and upstream regulators involved in inflammation and immune response, particularly those related to multiple sclerosis. A targeted survey of marine cyanobacterial samples from other geographic locations, including Guam, suggested the widespread occurrence of 1. Furthermore, the previous isolation of 1 from marine diatoms and green algae implied a potentially important ecological role across marine algal eukaryotes and prokaryotes. The previous isolation from sea lettuce raises the possibility of dietary intervention to attenuate inflammation and related disease progression.
Rhabdastrellosides A and B: Two New Isomalabaricane Glycosides from the Marine Sponge Rhabdastrella globostellata, and Their Cytotoxic and Cytoprotective Effects
Authors: Kozhushnaya Anastasia B. , Kolesnikova Sophia A. , Yurchenko Ekaterina A. , Lyakhova Ekaterina G. , Menshov Alexander S. , Kalinovsky Anatoly I. , Popov Roman S. , Dmitrenok Pavel S. , Ivanchina Natalia V.
Published: October 2023

Investigation of the Vietnamese marine sponge Rhabdastrella globostellata led to the isolation of two new polar isomalabaricanes: rhabdastrellosides A (1) and B (2). Their structures and stereochemistry were elucidated with the application of 1D and 2D NMR, HRESIMS, and HRESIMS/MS methods, as well as chemical modifications and GC–MS analysis. Metabolites 1 and 2 are the first isomalabaricanes with non-oxidized cyclopentane ring in the tricyclic core system. Moreover, having a 3-O-disaccharide moiety in their structures, they increase a very rare group of isomalabaricane glycosides. We report here a weak cytotoxicity of 1 and 2 toward human neuroblastoma SH-SY5Y cells and normal rat H9c2 cardiomyocytes, as well as the cytoprotective activity of rhabdastrelloside B (2) at 1 µM evaluated using CoCl2-treated SH-SY5Y and H9c2 cells.
Recovery of Nutrients from Cod Processing Waters
Authors: Coque Jorge , Jacobsen Charlotte , Forghani Bita , Meyer Anders , Jakobsen Greta , Sloth Jens J. , Sørensen Ann-Dorit Moltke
Published: October 2023

Liquid side-streams from food industries can be processed and used in food applications and contribute to reduce the environmental footprint of industries. The goal of this study was to evaluate the effectiveness and applicability of protein and phosphorus separation processes, namely microfiltration, ultrafiltration and flocculation, using protein-rich process waters with low (LS) and high (HS) salt content from the processing of salted cod (Gadus morhua). The application of different flocculants (chitosan lactate and Levasil RD442) were evaluated at different concentrations and maturation periods (0, 1 or 3 h). The results showed that different flocculation treatments resulted in different recoveries of the nutrients from LS and HS. Proteins in LS could be most efficiently recovered by using Levasil RD442 0.25% and no maturation period (51.4%), while phosphorus was most efficiently recovered when using Levasil RD442 1.23% and a maturation period of 1 h (34.7%). For HS, most of its protein was recovered using Levasil RD442 1.23% and a maturation period of 1 h (51.8%), while phosphorus was recovered the most using Levasil 1.23% and no maturation period (47.1%). The salt contents allowed interactions through intermolecular forces with Levasil RD442. The ultrafiltration method was effective on HS since it recovered higher percentages of nutrients in the retentate phase (57% of the protein and 46% of the phosphorus) compared to LS.
Real-Time and Rapid Respiratory Response of the Soil Microbiome to Moisture Shifts
Authors: Smith Montana L. , Weitz Karl K. , Thompson Allison M. , Jansson Janet K. , Hofmockel Kirsten S. , Lipton Mary S.
Published: October 2023

Microbial response to changing environmental factors influences the fate of soil organic carbon, and drought has been shown to affect microbial metabolism and respiration. We hypothesized that the access of microbes to different carbon pools in response to dry–rewet events occurs sequentially at different rates. We amended desiccated soils with 13C-labeled glucose and measured the rates of 12CO2 and 13CO2 respiration in real time after rewetting. Using these differentiated 12CO2 and 13CO2 respiration rate soils after rewetting, we were able to deduce when microbes are accessing different pools of carbon. Immediately upon rewetting, respiration of 12CO2 occurred first, with negligible 13CO2 respiration. Appreciable metabolism and respiration of the added 13C glucose did not occur until 15 min after rewetting. We conclude that, while all carbon pools are being accessed in the first 9 h after rewetting, the rate and timing at which new and existing carbon pools are being accessed varies. Within this study, using stable isotope-labeled substrates to discern which carbon pools are metabolized first uniquely illustrates how microorganisms access different carbon pools which has implications into understanding how carbon metabolism can further affect climate, carbon sequestration, and soil health.
Changes in Soil Bacterial Community and Function in Winter Following Long-Term Nitrogen (N) Deposition in Wetland Soil in Sanjiang Plain, China
Authors: Zhang Rongtao , Fu Xiaoyu , Zhong Haixiu , Sui Xin , Liu Yingnan
Published: October 2023

N deposition is a key factor affecting the composition and function of soil microbial communities in wetland ecosystems. Previous studies mainly focused on the effects of N deposition in the soil during the growing season (summer and autumn). Here, we focused on the response of the soil microbial community structure and function in winter. Soil from the Sanjiang Plain wetland, China, that had been treated for the past 11 years by using artificial N deposition at three levels (no intervention in N0, N deposition with 4 g N m−2 yr−1 in N1, and with 8 g N m−2 yr−1 in N2). Soil characteristics were determined and the bacterial composition and function was characterized using high-throughput sequence technology. The N deposition significantly reduced the soil bacterial diversity detected in winter compared with the control N0, and it significantly changed the composition of the bacterial community. At the phylum level, the high N deposition (N2) increased the relative abundance of Acidobacteria and decreased that of Myxococcota and Gemmatimonadota compared with N0. In soil from N2, the relative abundance of the general Candidatus_Solibacter and Bryobacter was significantly increased compared with N0. Soil pH, soil organic carbon (SOC), and total nitrogen (TN) were the key factors affecting the soil bacterial diversity and composition in winter. Soil pH was correlated with soil carbon cycling, probably due to its significant correlation with aerobic_chemoheterotrophy. The results show that a long-term N deposition reduces soil nutrients in winter wetlands and decreases soil bacterial diversity, resulting in a negative impact on the Sanjiang plain wetland. This study contributes to a better understanding of the winter responses of soil microbial community composition and function to the N deposition in temperate wetland ecosystems.
Study on Purification, Identification and Antioxidant of Flavonoids Extracted from Perilla leaves
Authors: Li Hui , Lin Jiayu , Bai Baoqing , Bo Tao , He Yufei , Fan Shanhong , Zhang Jinhua
Published: October 2023

The flavonoids from Perilla leaves were extracted using flash extraction assisted by ultrasonic extraction with ethanol. Subsequently, macroporous resin was employed for the isolation and purification of these flavonoids, followed by an investigation into their antioxidant activity. The process conditions for the extraction of flavonoids from Perilla leaves were designed and optimized using a one-way experiment combined with a response surface methodology. The optimal extraction conditions were determined as follows: the liquid–solid ratio was 20:1, ethanol volume fraction of 60%, ultrasound temperature of 60 °C, ultrasound time of 10 min and flash evaporation time of 60 s. The optimal extraction rate of flavonoids is 9.8 mg/g. In terms of separation and purification, a high-performance macroporous resin (HPD450 resin) with high purification efficiency was selected through static analysis and adsorption experiments. The optimal enrichment conditions were as follows: loading concentration of 0.06 mg/mL, optimal loading concentration of 20 mL, elution concentration of 70% and 76 mL, providing a reference for the further development and utilization of Perilla leaf flavonoids.
Special Issue “Frontiers in Nucleic Acid Chemistry—In Memory of Professor Enrique Pedroso for His Outstanding Contributions to Nucleic Acid Chemistry”
Authors: Terrazas Montserrat , Eritja Ramon , Montesarchio Daniela
Published: October 2023

This Special issue is dedicated to the memory of Enrique Pedroso, Professor Emeritus of Organic Chemistry at University of Barcelona, who passed away at the age of 72 in September 2020 [...]
Secrecy Performance Analysis of Mixed RF/FSO Systems Based on RIS Reflection Interference Eavesdropper
Authors: Wang Yong , Wang Yi , Lu Wangyue
Published: October 2023

This paper proposes and studies the physical layer security of a mixed radio frequency/free space optical (RF/FSO) system based on reconfigurable intelligent surface (RIS)-aided jamming to prevent eavesdropping. This work considers Nakagami-m fading for the RF links and Málaga (M) turbulence for the FSO links. A two-hop decode-and-forward (DF) relaying method was used and the eavesdropper actively eavesdropped on the information transmitted by the RF link. The eavesdropper was thwarted by a wireless-powered jammer that transmits jamming signals, which were reflected by the RIS to the eavesdropper to ensure secure communication in the mixed RF/FSO system. The expressions of secrecy outage probability (SOP) and average secrecy capacity (ASC) of the RIS-aided mixed RF/FSO system were derived for the system model discussed above. The Monte Carlo method was utilized to verify the accuracy of these expressions. In the RIS-aided mixed RF/FSO system, the effects of the time switching factor, energy conversion efficiency, and average interference noise ratio on the system secrecy outage probability were analyzed and compared to the RIS-free mixed RF/FSO system. Meanwhile, the influence of the number of RIS reflecting elements, link distances before and after reflection, and fading severity parameter on the security performance of a mixed RF/FSO system assisted by RIS were also investigated.