Neuronal wiring diagram of an adult brain
Authors: Dorkenwald Sven , Matsliah Arie , Sterling Amy R. , Schlegel Philipp , Yu Szi-chieh , McKellar Claire E. , Lin Albert , Costa Marta , Eichler Katharina , Yin Yijie , Silversmith Will , Schneider-Mizell Casey , Jordan Chris S. , Brittain Derrick , Halageri Akhilesh , Kuehner Kai , Ogedengbe Oluwaseun , Morey Ryan , Gager Jay , Kruk Krzysztof , Perlman Eric , Yang Runzhe , Deutsch David , Bland Doug , Sorek Marissa , Lu Ran , Macrina Thomas , Lee Kisuk , Bae J. Alexander , Mu Shang , Nehoran Barak , Mitchell Eric , Popovych Sergiy , Wu Jingpeng , Jia Zhen , Castro Manuel A. , Kemnitz Nico , Ih Dodam , Bates Alexander Shakeel , Eckstein Nils , Funke Jan , Collman Forrest , Bock Davi D. , Jefferis Gregory S. X. E. , Seung H. Sebastian , Murthy Mala ,   The FlyWire Consortium, Lenizo Zairene , Burke Austin T. , Willie Kyle Patrick , Serafetinidis Nikitas , Hadjerol Nashra , Willie Ryan , Silverman Ben , Ocho John Anthony , Bañez Joshua , Candilada Rey Adrian , Kristiansen Anne , Panes Nelsie , Yadav Arti , Tancontian Remer , Serona Shirleyjoy , Dolorosa Jet Ivan , Vinson Kendrick Joules , Garner Dustin , Salem Regine , Dagohoy Ariel , Skelton Jaime , Lopez Mendell , Capdevila Laia Serratosa , Badalamente Griffin , Stocks Thomas , Pandey Anjali , Akiatan Darrel Jay , Hebditch James , David Celia , Sapkal Dharini , Monungolh Shaina Mae , Sane Varun , Pielago Mark Lloyd , Albero Miguel , Laude Jacquilyn , dos Santos Márcia , Vohra Zeba , Wang Kaiyu , Gogo Allien Mae , Kind Emil , Mandahay Alvin Josh , Martinez Chereb , Asis John David , Nair Chitra , Patel Dhwani , Manaytay Marchan , Tamimi Imaan F. M. , Lim Clyde Angelo , Ampo Philip Lenard , Pantujan Michelle Darapan , Javier Alexandre , Bautista Daril , Rana Rashmita , Seguido Jansen , Parmar Bhargavi , Saguimpa John Clyde , Moore Merlin , Pleijzier Markus William , Larson Mark , Hsu Joseph , Joshi Itisha , Kakadiya Dhara , Braun Amalia , Pilapil Cathy , Gkantia Marina , Parmar Kaushik , Vanderbeck Quinn , Salgarella Irene , Dunne Christopher , Munnelly Eva , Kang Chan Hyuk , Lörsch Lena , Lee Jinmook , Kmecova Lucia , Sancer Gizem , Baker Christa , Joroff Jenna , Calle Steven , Patel Yashvi , Sato Olivia , Fang Siqi , Salocot Janice , Salman Farzaan , Molina-Obando Sebastian , Brooks Paul , Bui Mai , Lichtenberger Matthew , Tamboboy Edward , Molloy Katie , Santana-Cruz Alexis E. , Hernandez Anthony , Yu Seongbong , Diwan Arzoo , Patel Monika , Aiken Travis R. , Morejohn Sarah , Koskela Sanna , Yang Tansy , Lehmann Daniel , Chojetzki Jonas , Sisodiya Sangeeta , Koolman Selden , Shiu Philip K. , Cho Sky , Bast Annika , Reicher Brian , Blanquart Marlon , Houghton Lucy , Choi Hyungjun , Ioannidou Maria , Collie Matt , Eckhardt Joanna , Gorko Benjamin , Guo Li , Zheng Zhihao , Poh Alisa , Lin Marina , Taisz István , Murfin Wes , Díez Álvaro Sanz , Reinhard Nils , Gibb Peter , Patel Nidhi , Kumar Sandeep , Yun Minsik , Wang Megan , Jones Devon , Encarnacion-Rivera Lucas , Oswald Annalena , Jadia Akanksha , Erginkaya Mert , Drummond Nik , Walter Leonie , Tastekin Ibrahim , Zhong Xin , Mabuchi Yuta , Figueroa Santiago Fernando J. , Verma Urja , Byrne Nick , Kunze Edda , Crahan Thomas , Margossian Ryan , Kim Haein , Georgiev Iliyan , Szorenyi Fabianna , Adachi Atsuko , Bargeron Benjamin , Stürner Tomke , Demarest Damian , Gür Burak , Becker Andrea N. , Turnbull Robert , Morren Ashley , Sandoval Andrea , Moreno-Sanchez Anthony , Pacheco Diego A. , Samara Eleni , Croke Haley , Thomson Alexander , Laughland Connor , Dutta Suchetana B. , de Antón Paula Guiomar Alarcón , Huang Binglin , Pujols Patricia , Haber Isabel , González-Segarra Amanda , Choe Daniel T. , Lukyanova Veronika , Mancini Nino , Liu Zequan , Okubo Tatsuo , Flynn Miriam A. , Vitelli Gianna , Laturney Meghan , Li Feng , Cao Shuo , Manyari-Diaz Carolina , Yim Hyunsoo , Duc Le Anh , Maier Kate , Yu Seungyun , Nam Yeonju , Bąba Daniel , Abusaif Amanda , Francis Audrey , Gayk Jesse , Huntress Sommer S. , Barajas Raquel , Kim Mindy , Cui Xinyue , Sterne Gabriella R. , Li Anna , Park Keehyun , Dempsey Georgia , Mathew Alan , Kim Jinseong , Kim Taewan , Wu Guan-ting , Dhawan Serene , Brotas Margarida , Zhang Cheng-hao , Bailey Shanice , Del Toro Alexander , Yang Runzhe , Gerhard Stephan , Champion Andrew , Anderson David J. , Behnia Rudy , Bidaye Salil S. , Borst Alexander , Chiappe Eugenia , Colodner Kenneth J. , Dacks Andrew , Dickson Barry , Garcia Denise , Hampel Stefanie , Hartenstein Volker , Hassan Bassem , Helfrich-Forster Charlotte , Huetteroth Wolf , Kim Jinseop , Kim Sung Soo , Kim Young-Joon , Kwon Jae Young , Lee Wei-Chung , Linneweber Gerit A. , Maimon Gaby , Mann Richard , Noselli Stéphane , Pankratz Michael , Prieto-Godino Lucia , Read Jenny , Reiser Michael , von Reyn Katie , Ribeiro Carlos , Scott Kristin , Seeds Andrew M. , Selcho Mareike , Silies Marion , Simpson Julie , Waddell Scott , Wernet Mathias F. , Wilson Rachel I. , Wolf Fred W. , Yao Zepeng , Yapici Nilay , Zandawala Meet 
Published: October 2024

Connections between neurons can be mapped by acquiring and analysing electron microscopic brain images. In recent years, this approach has been applied to chunks of brains to reconstruct local connectivity maps that are highly informative1–6, but nevertheless inadequate for understanding brain function more globally. Here we present a neuronal wiring diagram of a whole brain containing 5 × 107 chemical synapses7 between 139,255 neurons reconstructed from an adult female Drosophila melanogaster8,9. The resource also incorporates annotations of cell classes and types, nerves, hemilineages and predictions of neurotransmitter identities10–12. Data products are available for download, programmatic access and interactive browsing and have been made interoperable with other fly data resources. We derive a projectome—a map of projections between regions—from the connectome and report on tracing of synaptic pathways and the analysis of information flow from inputs (sensory and ascending neurons) to outputs (motor, endocrine and descending neurons) across both hemispheres and between the central brain and the optic lobes. Tracing from a subset of photoreceptors to descending motor pathways illustrates how structure can uncover putative circuit mechanisms underlying sensorimotor behaviours. The technologies and open ecosystem reported here set the stage for future large-scale connectome projects in other species.