bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Cyclic Crack Opening-Closure during Epoxy-Curing Period of a CFRP Strengthening System Bonded on Concrete Substrate
Authors: Quiertant Marc , Boulay Claude , Siegert Laurent , Tourneur Christian
Published: January 2022

This article investigates the potential detrimental effects of cyclic load during the installation of externally bonded (EB) carbon fiber-reinforced polymer (CFRP) on a damaged reinforced concrete (RC) structure. Four RC specimens were tested in three point bending to study the consequences of crack cyclic opening-closure during epoxy-curing period. A first RC specimen (without bonded CFRP) was loaded monotonically up to failure to serve as undamaged control sample. The three other specimens were pre-cracked before being subjected to a fatigue loading procedure to simulate service condition of a damaged RC structure. Two of the three pre-cracked specimens were strengthened by EB CFRP. One specimen was repaired before the fatigue test while the other one was repaired during the fatigue test. Finally, remaining capacities of all three pre-cracked specimens were measured through monotonic bending tests until failure. It was found that, although bonding of CFRP reinforcement during cyclic load can induce some interesting features with regard to serviceability, cyclic crack opening and closing alters the cure process of epoxy located below the initial crack and decreases the effectiveness of the strengthening at ultimate state. Extended experimental studies are then needed to assess reliable safety factor for the design of repairing operations in which the bridge has to be maintained in service during CFRP installation.
Level Structure and Properties of Open f-Shell Elements
Authors: Fritzsche Stephan
Published: January 2022

Open f-shell elements still constitute a great challenge for atomic theory owing to their (very) rich fine-structure and strong correlations among the valence-shell electrons. For these medium and heavy elements, many atomic properties are sensitive to the correlated motion of electrons and, hence, require large-scale computations in order to deal consistently with all relativistic, correlation and rearrangement contributions to the electron density. Often, different concepts and notations need to be combined for just classifying the low-lying level structure of these elements. With Jac, the Jena Atomic Calculator, we here provide a toolbox that helps to explore and deal with such elements with open d- and f-shell structures. Based on Dirac’s equation, Jac is suitable for almost all atoms and ions across the periodic table. As an example, we demonstrate how reasonably accurate computations can be performed for the low-lying level structure, transition probabilities and lifetimes for Th2+ ions with a 5f6d ground configuration. Other, and more complex, shell structures are supported as well, though often for a trade-off between the size and accuracy of the computations. Owing to its simple use, however, Jac supports both quick estimates and detailed case studies on open d- or f-shell elements.
The Impact of the Extracellular Matrix Environment on Sost Expression by the MLO-Y4 Osteocyte Cell Line
Authors: Brady Robert T. , O’Brien Fergal J. , Hoey David A.
Published: January 2022

Bone is a dynamic organ that can adapt its structure to meet the demands of its biochemical and biophysical environment. Osteocytes form a sensory network throughout the tissue and orchestrate tissue adaptation via the release of soluble factors such as a sclerostin. Osteocyte physiology has traditionally been challenging to investigate due to the uniquely mineralized extracellular matrix (ECM) of bone leading to the development of osteocyte cell lines. Importantly, the most widely researched and utilized osteocyte cell line: the MLO-Y4, is limited by its inability to express sclerostin (Sost gene) in typical in-vitro culture. We theorised that culture in an environment closer to the in vivo osteocyte environment could impact on Sost expression. Therefore, this study investigated the role of composition and dimensionality in directing Sost expression in MLO-Y4 cells using collagen-based ECM analogues. A significant outcome of this study is that MLO-Y4 cells, when cultured on a hydroxyapatite (HA)-containing two-dimensional (2D) film analogue, expressed Sost. Moreover, three-dimensional (3D) culture within HA-containing collagen scaffolds significantly enhanced Sost expression, demonstrating the impact of ECM composition and dimensionality on MLO-Y4 behaviour. Importantly, in this bone mimetic ECM environment, Sost expression was found to be comparable to physiological levels. Lastly, MLO-Y4 cells cultured in these novel conditions responded accordingly to fluid flow stimulation with a decrease in expression. This study therefore presents a novel culture system for the MLO-Y4 osteocyte cell line, ensuring the expression of an important osteocyte specific gene, Sost, overcoming a major limitation of this model.
Flexible Strain-Sensitive Silicone-CNT Sensor for Human Motion Detection
Authors: Demidenko Natalia A. , Kuksin Artem V. , Molodykh Victoria V. , Pyankov Evgeny S. , Ichkitidze Levan P. , Zaborova Victoria A. , Tsymbal Alexandr A. , Tkachenko Svetlana A. , Shafaei Hassan , Diachkova Ekaterina , Gerasimenko Alexander Yu.
Published: January 2022

This article describes the manufacturing technology of biocompatible flexible strain-sensitive sensor based on Ecoflex silicone and multi-walled carbon nanotubes (MWCNT). The sensor demonstrates resistive behavior. Structural, electrical, and mechanical characteristics are compared. It is shown that laser radiation significantly reduces the resistance of the material. Through laser radiation, electrically conductive networks of MWCNT are formed in a silicone matrix. The developed sensor demonstrates highly sensitive characteristics: gauge factor at 100% elongation −4.9, gauge factor at 90° bending −0.9%/deg, stretchability up to 725%, tensile strength 0.7 MPa, modulus of elasticity at 100% 46 kPa, and the temperature coefficient of resistance in the range of 30–40 °C is −2 × 10−3. There is a linear sensor response (with 1 ms response time) with a low hysteresis of ≤3%. An electronic unit for reading and processing sensor signals based on the ATXMEGA8E5-AU microcontroller has been developed. The unit was set to operate the sensor in the range of electrical resistance 5–150 kOhm. The Bluetooth module made it possible to transfer the received data to a personal computer. Currently, in the field of wearable technologies and health monitoring, a vital need is the development of flexible sensors attached to the human body to track various indicators. By integrating the sensor with the joints of the human hand, effective movement sensing has been demonstrated.
Canthaxanthin Biofabrication, Loading in Green Phospholipid Vesicles and Evaluation of In Vitro Protection of Cells and Promotion of Their Monolayer Regeneration
Authors: Castangia Ines , Manca Maria Letizia , Razavi Seyed Hadi , Nácher Amparo , Díez-Sales Octavio , Peris José Esteban , Allaw Mohamad , Terencio Maria Carmen , Usach Iris , Manconi Maria
Published: January 2022

In the present study, canthaxanthin was produced by biofermentation from Dietzia natronolimnaea HS-1 (D. natronolimnaea) and was loaded in phospholipid vesicles prepared with natural component using an easy and low dissipative method. Indeed, glycerosomes, hyalurosomes, and glycerohyalurosomes were prepared by direct hydration of both phosphatidylcholine and the biotechnological canthaxanthin, avoiding the use of organic solvents. Vesicles were sized from 63 nm to 87 nm and highly negatively charged. They entrapped a high number of the biomolecules and were stable on storage. Canthaxanthin-loaded vesicles incubated with fibroblasts did not affect their viability, proving to be highly biocompatible and capable of inhibiting the death of fibroblasts stressed with hydrogen peroxide. They reduced the nitric oxide expression in macrophages treated with lipopolysaccharides. Moreover, they favoured the cell migration in an in vitro lesion model. Results confirmed the health-promoting potential of canthaxanthin in skin cells, which is potentiated by its suitable loading in phospholipid vesicles, thus suggesting the possible use of these natural bioformulations in both skin protection and regeneration, thanks to the potent antioxidant, anti-inflammatory and antiageing effects of canthaxanthin.
Phenotypic Heterogeneity among GBA p.R202X Carriers in Lewy Body Spectrum Disorders
Authors: Napolioni Valerio , Fredericks Carolyn A. , Kim Yongha , Channappa Divya , Khan Raiyan R. , Kim Lily H. , Zafar Faria , Couthouis Julien , Davidzon Guido A. , Mormino Elizabeth C. , Gitler Aaron D. , Montine Thomas J. , Schüle Birgitt , Greicius Michael D.
Published: January 2022

We describe the clinical and neuropathologic features of patients with Lewy body spectrum disorder (LBSD) carrying a nonsense variant, c.604C>T; p.R202X, in the glucocerebrosidase 1 (GBA) gene. While this GBA variant is causative for Gaucher’s disease, the pathogenic role of this mutation in LBSD is unclear. Detailed neuropathologic evaluation was performed for one index case and a structured literature review of other GBA p.R202X carriers was conducted. Through the systematic literature search, we identified three additional reported subjects carrying the same GBA mutation, including one Parkinson’s disease (PD) patient with early disease onset, one case with neuropathologically-verified LBSD, and one unaffected relative of a Gaucher’s disease patient. Among the affected subjects carrying the GBA p.R202X, all males were diagnosed with Lewy body dementia, while the two females presented as PD. The clinical penetrance of GBA p.R202X in LBSD patients and families argues strongly for a pathogenic role for this variant, although presenting with a striking phenotypic heterogeneity of clinical and pathological features.
From NAFLD to MAFLD: Aligning Translational In Vitro Research to Clinical Insights
Authors: Gatzios Alexandra , Rombaut Matthias , Buyl Karolien , De Kock Joery , Rodrigues Robim M. , Rogiers Vera , Vanhaecke Tamara , Boeckmans Joost
Published: January 2022

Although most same-stage non-alcoholic fatty liver disease (NAFLD) patients exhibit similar histologic sequelae, the underlying mechanisms appear to be highly heterogeneous. Therefore, it was recently proposed to redefine NAFLD to metabolic dysfunction-associated fatty liver disease (MAFLD) in which other known causes of liver disease such as alcohol consumption or viral hepatitis do not need to be excluded. Revised nomenclature envisions speeding up and facilitating anti-MAFLD drug development by means of patient stratification whereby each subgroup would benefit from distinct pharmacological interventions. As human-based in vitro research fulfils an irrefutable step in drug development, action should be taken as well in this stadium of the translational path. Indeed, most established in vitro NAFLD models rely on short-term exposure to fatty acids and use lipid accumulation as a phenotypic benchmark. This general approach to a seemingly ambiguous disease such as NAFLD therefore no longer seems applicable. Human-based in vitro models that accurately reflect distinct disease subgroups of MAFLD should thus be adopted in early preclinical disease modeling and drug testing. In this review article, we outline considerations for setting up translational in vitro experiments in the MAFLD era and allude to potential strategies to implement MAFLD heterogeneity into an in vitro setting so as to better align early drug development with future clinical trial designs.
Recent Developments in Clinical Plasma Proteomics—Applied to Cardiovascular Research
Authors: Palstrøm Nicolai Bjødstrup , Matthiesen Rune , Rasmussen Lars Melholt , Beck Hans Christian
Published: January 2022

The human plasma proteome mirrors the physiological state of the cardiovascular system, a fact that has been used to analyze plasma biomarkers in routine analysis for the diagnosis and monitoring of cardiovascular diseases for decades. These biomarkers address, however, only a very limited subset of cardiovascular diseases, such as acute myocardial infarct or acute deep vein thrombosis, and clinical plasma biomarkers for the diagnosis and stratification cardiovascular diseases that are growing in incidence, such as heart failure and abdominal aortic aneurysm, do not exist and are urgently needed. The discovery of novel biomarkers in plasma has been hindered by the complexity of the human plasma proteome that again transforms into an extreme analytical complexity when it comes to the discovery of novel plasma biomarkers. This complexity is, however, addressed by recent achievements in technologies for analyzing the human plasma proteome, thereby facilitating the possibility for novel biomarker discoveries. The aims of this article is to provide an overview of the recent achievements in technologies for proteomic analysis of the human plasma proteome and their applications in cardiovascular medicine.
Systemic Sclerosis: From Pathophysiology to Novel Therapeutic Approaches
Authors: Benfaremo Devis , Svegliati Silvia , Paolini Chiara , Agarbati Silvia , Moroncini Gianluca
Published: January 2022

Systemic sclerosis (SSc) is a systemic, immune-mediated chronic disorder characterized by small vessel alterations and progressive fibrosis of the skin and internal organs. The combination of a predisposing genetic background and triggering factors that causes a persistent activation of immune system at microvascular and tissue level is thought to be the pathogenetic driver of SSc. Endothelial alterations with subsequent myofibroblast activation, excessive extracellular matrix (ECM) deposition, and unrestrained tissue fibrosis are the pathogenetic steps responsible for the clinical manifestations of this disease, which can be highly heterogeneous according to the different entity of each pathogenic step in individual subjects. Although substantial progress has been made in the management of SSc in recent years, disease-modifying therapies are still lacking. Several molecular pathways involved in SSc pathogenesis are currently under evaluation as possible therapeutic targets in clinical trials. These include drugs targeting fibrotic and metabolic pathways (e.g., TGF-β, autotaxin/LPA, melanocortin, and mTOR), as well as molecules and cells involved in the persistent activation of the immune system (e.g., IL4/IL13, IL23, JAK/STAT, B cells, and plasma cells). In this review, we provide an overview of the most promising therapeutic targets that could improve the future clinical management of SSc.
Microtubule Affinity-Regulating Kinase 4 Promotes Oxidative Stress and Mitochondrial Dysfunction by Activating NF-κB and Inhibiting AMPK Pathways in Porcine Placental Trophoblasts
Authors: Tian Liang , Liu Guangfan , Kang Ziqi , Yan Peishi
Published: January 2022

The aim of this investigation was to evaluate the role of MARK4 in the regulation of oxidative stress and mitochondrial dysfunction in pig placental trophoblasts and analyze the signaling pathways involved. In this study, we found that enhanced MARK4 contributed to augmented oxidative stress in pig trophoblasts, as evidenced by decreased total antioxidant capacity (TAC); higher production of reactive oxygen species (ROS); elevated protein carbonylation; and reduced SOD, CAT, and GSH-PX activities. Further analyses revealed MARK4 impaired mitochondrial oxidative respiration in cultured trophoblasts, which was associated with reduced ATP content, decreased mitochondrial membrane potential, lower mitochondrial Complexes I and III activities, and down-regulated protein contents of subunits of complexes I, II, and V. At same time, mitochondrial biogenesis and structure were negatively altered by elevated MARK4. By antioxidant treatment with vitamin E (VE), oxidative stress along with impaired mitochondrial function induced by enhanced MARK4 were blocked. Furthermore, we found activation of AMPK signaling prevented MARK4 from blocking mitochondrial biogenesis and function in pig trophoblast cells. Finally, we demonstrated that the IKKα/NF-κB signal pathway was involved in MARK4 activated oxidative stress and mitochondrial dysfunction. Thus, these data suggest that MARK4 promotes oxidative stress and mitochondrial injury in porcine placental trophoblasts and can contribute to the developing of knowledge of pathological processes leading to mitochondrial dysfunction associated with excessive back-fat in the pig placenta and to the obesity-associated pregnant syndrome.
Comparison of Diagnostic Values of Maternal Arginine Concentration for Different Pregnancy Complications: A Systematic Review and Meta-Analysis
Authors: Xu Lianbin , Zeng Jia , Wang Huanan , Liu Hongyun
Published: January 2022

Abnormal arginine metabolism contributes to the development of intrauterine growth restriction (IUGR), preeclampsia (PE), and gestational diabetes mellitus (GDM), which increase the health burden of mothers and induce adverse birth outcomes. However, associations between maternal arginine concentration and different pregnancy complications have not been systematically compared. The PubMed, ScienceDirect, and Web of Science databases were searched for peer-reviewed publications to evaluate the diagnostic value of plasma arginine concentration in complicated pregnancies. Standardized mean difference (SMD) of the arginine concentration was pooled by a random effects model. The results show that increased maternal arginine concentrations were observed in IUGR (SMD: 0.48; 95% CI: 0.20, 0.76; I2 = 47.0%) and GDM (SMD: 0.46; 95% CI: 0.11, 0.81; I2 = 82.3%) cases but not in PE patients (SMD: 0.21; 95% CI: −0.04, 0.47; I2 = 80.3%) compared with the normal cohorts. Subgroup analyses indicated that the non-fasting circulating arginine concentration in third trimester was increased significantly in GDM and severe IUGR pregnancies, but the change mode was dependent on ethnicity. Additionally, only severe PE persons were accompanied by higher plasma arginine concentrations. These findings suggest that maternal arginine concentration is an important reference for assessing the development of pregnancy complications.
Recent Advances in Computational Modeling of Biomechanics and Biorheology of Red Blood Cells in Diabetes
Authors: Deng Yi-Xiang , Chang Hung-Yu , Li He
Published: January 2022

Diabetes mellitus, a metabolic disease characterized by chronically elevated blood glucose levels, affects about 29 million Americans and more than 422 million adults all over the world. Particularly, type 2 diabetes mellitus (T2DM) accounts for 90–95% of the cases of vascular disease and its prevalence is increasing due to the rising obesity rates in modern societies. Although multiple factors associated with diabetes, such as reduced red blood cell (RBC) deformability, enhanced RBC aggregation and adhesion to the endothelium, as well as elevated blood viscosity are thought to contribute to the hemodynamic impairment and vascular occlusion, clinical or experimental studies cannot directly quantify the contributions of these factors to the abnormal hematology in T2DM. Recently, computational modeling has been employed to dissect the impacts of the aberrant biomechanics of diabetic RBCs and their adverse effects on microcirculation. In this review, we summarize the recent advances in the developments and applications of computational models in investigating the abnormal properties of diabetic blood from the cellular level to the vascular level. We expect that this review will motivate and steer the development of new models in this area and shift the attention of the community from conventional laboratory studies to combined experimental and computational investigations, aiming to provide new inspirations for the development of advanced tools to improve our understanding of the pathogenesis and pathology of T2DM.
Presbycusis and the Aging of Eye Movement: Common Attention Mechanisms
Authors: Chavant Martin , Kapoula Zoï
Published: January 2022

Presbycusis, physiological age-related hearing loss, is a major health problem because it is the most common cause of hearing impairment, and its impact will grow in the coming years with the aging population. Besides auditory consequences, the literature recently found an association between hearing loss and cognitive decline over the last two decades, emphasizing the importance of the early detection of presbycusis. However, the current hearing tests are not sufficient to detect presbycusis in some cases. Furthermore, the underlying mechanisms of this association are still under discussion, calling for a new field of research on that topic. In that context, this study investigates for the first time the interaction between presbycusis, eye movement latency and Stroop scores for a normal aging population. Hearing abilities, eye movement latency and the Stroop Victoria test were measured for 69 elderly (mean 66.7 ± 8.4) and 30 young (mean 25.3 ± 2.7) participants. The results indicated a significant relationship between saccade latency and speech audiometry in the silence score, independently from age. These promising results suggest common attentional mechanisms between speech processing and saccade latency. The results are discussed regarding the relationship between hearing and cognition, and regarding the perspective of expanding new tools for presbycusis diagnosis.
Tfeb-Mediated Transcriptional Regulation of Autophagy Induces Autosis during Ischemia/Reperfusion in the Heart
Authors: Nah Jihoon , Sung Eun-Ah , Zhai Peiyong , Zablocki Daniela , Sadoshima Junichi
Published: January 2022

Autosis is a unique form of cell death with characteristic morphological and biochemical features caused by dysregulated autophagy. Autosis is observed in the heart during the late phase of ischemia/reperfusion (I/R), when marked accumulation of autophagosomes is induced. We previously showed that the excessive accumulation of autophagosomes promotes autosis in cardiomyocytes. Although the inhibition of autophagic flux via the upregulation of Rubicon induces the accumulation of autophagosomes during I/R, it appears that additional mechanisms exacerbating autophagosome accumulation are required for the induction of autosis. Here, we show that Tfeb contributes to the induction of autosis during the late phase of I/R in the heart. During myocardial reperfusion, Tfeb is activated and translocated into the nucleus, which in turn upregulates genes involved in autophagy and lysosomal function. The overexpression of Tfeb enhanced cardiomyocyte death induced by a high dose of TAT-Beclin 1, an effect that was inhibited by the downregulation of Atg7. Conversely, the knockdown of Tfeb attenuated high-dose TAT-Beclin1-induced death in cardiomyocytes. Although the downregulation of Tfeb in the heart significantly decreased the number of autophagic vacuoles and inhibited autosis during I/R, the activation of Tfeb activity via 3,4-dimethoxychalcone, an activator of Tfeb, aggravated myocardial injury during I/R. These findings suggest that Tfeb promotes cardiomyocyte autosis during the late phase of reperfusion in the heart.
Tackling the Behavior of Cancer Cells: Molecular Bases for Repurposing Antipsychotic Drugs in the Treatment of Glioblastoma
Authors: Persico Michele , Abbruzzese Claudia , Matteoni Silvia , Matarrese Paola , Campana Anna Maria , Villani Veronica , Pace Andrea , Paggi Marco G.
Published: January 2022

Glioblastoma (GBM) is associated with a very dismal prognosis, and current therapeutic options still retain an overall unsatisfactorily efficacy in clinical practice. Therefore, novel therapeutic approaches and effective medications are highly needed. Since the development of new drugs is an extremely long, complex and expensive process, researchers and clinicians are increasingly considering drug repositioning/repurposing as a valid alternative to the standard research process. Drug repurposing is also under active investigation in GBM therapy, since a wide range of noncancer and cancer therapeutics have been proposed or investigated in clinical trials. Among these, a remarkable role is played by the antipsychotic drugs, thanks to some still partially unexplored, interesting features of these agents. Indeed, antipsychotic drugs have been described to interfere at variable incisiveness with most hallmarks of cancer. In this review, we analyze the effects of antipsychotics in oncology and how these drugs can interfere with the hallmarks of cancer in GBM. Overall, according to available evidence, mostly at the preclinical level, it is possible to speculate that repurposing of antipsychotics in GBM therapy might contribute to providing potentially effective and inexpensive therapies for patients with this disease.
Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis
Authors: Qin Mengyao , Xin Yu , Bian Yong , Yang Xuan , Xi Tao , Xiong Jing
Published: January 2022

Pregnane X receptor (PXR) is a member of the nuclear receptor superfamily that is activated by a variety of endogenous metabolites or xenobiotics. Its downstream target genes are involved in metabolism, inflammation and processes closely related to cancer. However, the stability regulation of PXR protein resulting from post-translational modification is still largely undefined. In the present study, primary mouse hepatocytes, hepatoma HepG2 cells and HEK 293T cells were used to investigate gene expression and protein interactions. The role of kinases was evaluated by RNA interference and overexpression constructs with or without PXR phosphorylation site mutations. The activity of CYP3A4 and P-gp was determined by enzymatic and substrate accumulation assays. It was found that E3 ubiquitin ligase TRIM21 mediates the ubiquitination and degradation of PXR and plays an important role in regulating the activity of PXR. On this basis, PXR phosphorylation-associated kinases were evaluated regarding regulation of the stability of PXR. We found cyclin dependent kinase 2 (CDK2) exclusively phosphorylates PXR at Ser350, promotes its disassociation with Hsp90/DNAJC7, and leads to subsequent TRIM21-mediated PXR ubiquitination and degradation. As well-known CDK inhibitors, dinaciclib and kenpaullone stabilize PXR and result in elevated expression and activity of PXR-targeted DMETs, including carboxylesterases, CYP3A4 and P-gp. The suppressed degradation of PXR by CDK2 inhibitors denotes dinaciclib-induced promotion of PXR-targeted genes. The findings of CDK2-mediated PXR degradation indicate a wide range of potential drug–drug interactions during clinical cancer therapy using CDK inhibitors and imply an alternative direction for the development of novel PXR antagonists.
Selective Interactions of Mouse Melanocortin Receptor Accessory Proteins with Somatostatin Receptors
Authors: Wang Meng , Xu Jing , Lei Xiao-Wei , Zhang Cong , Liu Shang-Yun , Jin Li-Na , Zhang Chao
Published: January 2022

Somatostatin receptors (SSTRs) are G protein-coupled receptors (GPCRs) known to regulate exocrine secretion, neurotransmission, and inhibit endogenous cell proliferation. SSTR subtypes (SSTR1-SSTR5) exhibit homo- or heterodimerization with unique signaling characteristics. Melanocortin receptor accessory protein 1 (MRAP1) functions as an allosteric modulator of melanocortin receptors and some other GPCRs. In this study, we investigated the differential interaction of MRAP1 and SSTRs and examined the pharmacological modulation of MRAP1 on mouse SSTR2/SSTR3 and SSTR2/SSTR5 heterodimerization in vitro. Our results show that the mouse SSTR2 forms heterodimers with SSTR3 and SSTR5 and that MRAP1 selectively interacts with SSTR3 and SSTR5 but not SSTR2. The interactive binding sites of SSTR2/SSTR3 or SSTR2/SSTR5 with MRAP1 locate on SSTR3 and SSTR5 but not SSTR2. The binding sites of MRAP1 to SSTR3 are extensive, while the ones of SSTR5 are restricted on transmembrane region six and seven. The heterodimerization of mouse SSTR2, SSTR3, and SSTR5 can be modulated by binding protein in addition to an agonist. Upregulation of extracellular signal-regulated kinases phosphorylation, p27Kip1, and increased cell growth inhibition with the co-expression of SSTR2/SSTR3 or SSTR2/SSTR5 with MRAP1 suggest a regulatory effect of MRAP1 on anti-proliferative response of two SSTR heterodimers. Taken together, these results provide a new insight of MRAP1 on the maintenance and regulation of mouse SSTR dimers which might be helpful to better understand the molecular mechanism involving SSTRs in tumor biology or other human disorders.
A Practical Hydrazine-Carbothioamide-Based Fluorescent Probe for the Detection of Zn2+: Applications to Paper Strip, Zebrafish and Water Samples
Authors: Suh Boeon , Gil Dongkyun , Yoon Sojeong , Kim Ki-Tae , Kim Cheal
Published: January 2022

A practical hydrazine-carbothioamide-based fluorescent chemosensor TCC (N-(4-chlorophenyl)-2-(thiophene-2-carbonyl)hydrazine-1-carbothioamide) was applied for Zn2+ detection. TCC exhibited selective fluorescence emission for Zn2+ and did not show any interference with other metal ions. In particular, TCC was utilized for the detection of Zn2+ in paper strips, zebrafish and real water samples. TCC could detect Zn2+ down to 0.39 μM in the solution phase and 51.13 μM in zebrafish. The association ratio between TCC and Zn2+ was determined to be 2:1 by ESI-mass and Job plot. The sensing mechanism of TCC for Zn2+ was illustrated to be a chelation-enhanced fluorescence process through spectroscopic experiments and theoretical calculations.
Electrochemical Sensing of Idarubicin—DNA Interaction Using Electropolymerized Azure B and Methylene Blue Mediation
Authors: Goida Anastasia , Kuzin Yurii , Evtugyn Vladimir , Porfireva Anna , Evtugyn Gennady , Hianik Tibor
Published: January 2022

A highly sensitive electrochemical DNA sensor for detection of the chemotherapeutic drug idarubicin mediated by Methylene blue (MB) has been developed. DNA from fish sperm has been immobilized at the electropolymerized layers of Azure B. The incorporation of MB into the DNA layers substantially increased the sensor sensitivity. The concentration range for idarubicin determination by cyclic voltammetry was from 1 fM to 0.1 nM, with a limit of detection (LOD) of 0.3 fM. Electrochemical impedance spectroscopy (EIS) in the presence of a redox probe ([Fe(CN)6]3−/4−) allowed for the widening of a linear range of idarubicin detection from 1 fM to 100 nM, retaining LOD 0.3 fM. The DNA sensor has been tested in various real and artificial biological fluids with good recovery ranging between 90–110%. The sensor has been successfully used for impedimetric idarubicin detection in medical preparation Zavedos®. The developed DNA biosensor could be useful for the control of the level of idarubicin during cancer therapy as well as for pharmacokinetics studies.
Current Knowledge of Selected Cardiovascular Biomarkers in Pediatrics: Kidney Injury Molecule-1, Salusin-α and -β, Uromodulin, and Adropin
Authors: Močnik Mirjam , Marčun Varda Nataša
Published: January 2022

Cardiovascular diseases are the leading cause of morbidity and mortality in the modern world. Their common denominator is atherosclerosis, a process beginning in childhood. In pediatrics, the aim of preventive measures is to recognize children and adolescents at risk for accelerated atherosclerosis and possible premature cardiovascular events in adulthood. Several diagnostic procedures and biomarkers are available for cardiovascular risk assessment in adults. However, reliable markers in pediatrics are still insufficiently studied. In this contribution, we discuss five potential biomarkers of particular interest: kidney injury molecule-1, salusin-α and -β, uromodulin, and adropin. Studies regarding the pediatric population are scarce, but they support the evidence from studies in the adult population. These markers might entail both a prognostic and a therapeutic interest.
The Moderating Role of Surgency, Behavioral Inhibition, Negative Emotionality and Effortful Control in the Relationship between Parenting Style and Children’s Reactive and Proactive Aggression
Authors: del Puerto-Golzarri Nora , Azurmendi Aitziber , Carreras María Rosario , Muñoz José Manuel , Braza Paloma , Vegas Oscar , Pascual-Sagastizabal Eider
Published: January 2022

The principal aim of this study is to explore the moderating role of temperament in the relationship between parenting style and the reactive and proactive aggressive behavior of 8-year-old children. The participants are 279 children (154 boys and 125 girls). To measure reactive and proactive aggression, children completed the reactive and proactive questionnaire (RPQ). Child temperament and parenting styles were evaluated by both parents using the temperament in middle childhood questionnaire (TMCQ) and the parenting styles and dimensions questionnaire (PSDQ). The results revealed that boys with high surgency levels and authoritarian fathers displayed more reactive aggression, whereas behaviorally inhibited boys with mothers who scored low for authoritarian parenting displayed less reactive aggression. Finally, girls with high levels of effortful control and mothers who scored low for authoritative parenting displayed more proactive aggression. The results highlight the value of studying the moderating role of temperament in the relationship between children’s aggressive behavior and both mothers’ and fathers’ parenting styles, and underscores the importance of doing so separately for boys and girls.
An IoT System Using Deep Learning to Classify Camera Trap Images on the Edge
Authors: Zualkernan Imran , Dhou Salam , Judas Jacky , Sajun Ali Reza , Gomez Brylle Ryan , Hussain Lana Alhaj
Published: January 2022

Camera traps deployed in remote locations provide an effective method for ecologists to monitor and study wildlife in a non-invasive way. However, current camera traps suffer from two problems. First, the images are manually classified and counted, which is expensive. Second, due to manual coding, the results are often stale by the time they get to the ecologists. Using the Internet of Things (IoT) combined with deep learning represents a good solution for both these problems, as the images can be classified automatically, and the results immediately made available to ecologists. This paper proposes an IoT architecture that uses deep learning on edge devices to convey animal classification results to a mobile app using the LoRaWAN low-power, wide-area network. The primary goal of the proposed approach is to reduce the cost of the wildlife monitoring process for ecologists, and to provide real-time animal sightings data from the camera traps in the field. Camera trap image data consisting of 66,400 images were used to train the InceptionV3, MobileNetV2, ResNet18, EfficientNetB1, DenseNet121, and Xception neural network models. While performance of the trained models was statistically different (Kruskal–Wallis: Accuracy H(5) = 22.34, p < 0.05; F1-score H(5) = 13.82, p = 0.0168), there was only a 3% difference in the F1-score between the worst (MobileNet V2) and the best model (Xception). Moreover, the models made similar errors (Adjusted Rand Index (ARI) > 0.88 and Adjusted Mutual Information (AMU) > 0.82). Subsequently, the best model, Xception (Accuracy = 96.1%; F1-score = 0.87; F1-Score = 0.97 with oversampling), was optimized and deployed on the Raspberry Pi, Google Coral, and Nvidia Jetson edge devices using both TenorFlow Lite and TensorRT frameworks. Optimizing the models to run on edge devices reduced the average macro F1-Score to 0.7, and adversely affected the minority classes, reducing their F1-score to as low as 0.18. Upon stress testing, by processing 1000 images consecutively, Jetson Nano, running a TensorRT model, outperformed others with a latency of 0.276 s/image (s.d. = 0.002) while consuming an average current of 1665.21 mA. Raspberry Pi consumed the least average current (838.99 mA) with a ten times worse latency of 2.83 s/image (s.d. = 0.036). Nano was the only reasonable option as an edge device because it could capture most animals whose maximum speeds were below 80 km/h, including goats, lions, ostriches, etc. While the proposed architecture is viable, unbalanced data remain a challenge and the results can potentially be improved by using object detection to reduce imbalances and by exploring semi-supervised learning.
FPGA-Implemented Fractal Decoder with Forward Error Correction in Short-Reach Optical Interconnects
Authors: Matsenko Svitlana , Borysenko Oleksiy , Spolitis Sandis , Udalcovs Aleksejs , Gegere Lilita , Krotov Aleksandr , Ozolins Oskars , Bobrovs Vjaceslavs
Published: January 2022

Forward error correction (FEC) codes combined with high-order modulator formats, i.e., coded modulation (CM), are essential in optical communication networks to achieve highly efficient and reliable communication. The task of providing additional error control in the design of CM systems with high-performance requirements remains urgent. As an additional control of CM systems, we propose to use indivisible error detection codes based on a positional number system. In this work, we evaluated the indivisible code using the average probability method (APM) for the binary symmetric channel (BSC), which has the simplicity, versatility and reliability of the estimate, which is close to reality. The APM allows for evaluation and compares indivisible codes according to parameters of correct transmission, and detectable and undetectable errors. Indivisible codes allow for the end-to-end (E2E) control of the transmission and processing of information in digital systems and design devices with a regular structure and high speed. This study researched a fractal decoder device for additional error control, implemented in field-programmable gate array (FPGA) software with FEC for short-reach optical interconnects with multilevel pulse amplitude (PAM-M) modulated with Gray code mapping. Indivisible codes with natural redundancy require far fewer hardware costs to develop and implement encoding and decoding devices with a sufficiently high error detection efficiency. We achieved a reduction in hardware costs for a fractal decoder by using the fractal property of the indivisible code from 10% to 30% for different n while receiving the reciprocal of the golden ratio.
Spatial Market Integration: A Case Study of the Polish–Czech Milk Market
Authors: Roman Monika , Žáková Kroupová Zdeňka
Published: January 2022

Analyses of spatial market integration contributes to the knowledge about market efficiency and provides information to policymakers, as the spatial integration of markets contributes to competitiveness and economic development. Although the integration of agri-food markets is widely discussed in the economic literature, research on the dairy sector is relatively limited. This paper fulfils the research gap with an in-depth investigation of spatial milk and dairy product market integration between two neighboring countries—Poland and Czechia—using regional data, and including both production and processing levels. The econometric analysis of time series covering the period 2001–2021 reveals that only long-run milk and skimmed milk powder (SMP) price relationships are between the Czech Republic and Poland. The results of the study confirm that the factors influencing spatial price relationships between the Czech Republic and Poland are: strong trade ties, the common moment of accession to the EU, a close distance between markets, and region specialization.
Slug Flow Hydrodynamics Modeling for Gas–Liquid Two-Phase Flow in a Pipe
Authors: Liu Huishu , Duan Jimiao , Gu Kecheng , Li Jiang , Yan Hao , Wang Jian , Li Changjun
Published: January 2022

Gas–liquid flow in a pipeline is a very common. Slug two-phase flow is dominated in the case of slightly upward flow (+0.25°) and considered to be the comprehensive flow configuration, and can be in close contact with all the other flow patterns. The models of different flow patterns can be unified. Precise prediction of the slug flow is crucial for proper design and operation. In this paper, we develop hydrodynamics unified modeling for gas–liquid two-phase slug flow, and the bubble and droplet entrainment is optimized. For the important parameters (wall and interfacial friction factors, slug translational velocity and average slug length), the correlations of these parameters are optimized. Furthermore, the related parameters for liquid droplet and gas bubble entrainment are given. Accounting for the gas–liquid interface shape, hydrodynamics models, i.e., the flat interface model (FIM) and the double interface model (DIM), of liquid film in the slug body are applied and compared with the experimental data. The calculated results show that the predictions for the liquid holdup and pressure gradient of the DIM agree with experimental data better than those of the FIM. A comparison between the available experimental results and Zhang’s model calculations shows that the DIM model correctly describes the slug dynamics in gas–liquid pipe flow.
Genetic Algorithm Based Temperature-Queuing Method for Aggregated IAC Load Control
Authors: Chen Zexu , Shi Jing , Song Zhaofang , Yang Wangwang , Zhang Zitong
Published: January 2022

In recent years, demand response (DR) has played an increasingly important role in maintaining the safety, stability and economic operation of power grid. Due to the continuous running state and extremely fast speed of response, the aggregated inverter air conditioning (IAC) load is considered as the latest and most ideal object for DR. However, it is easy to cause load rebound when the aggregated IAC load participates in DR. Existing methods for controlling air conditioners to participate in DR cannot meet the following three requirements at the same time: basic DR target, load rebound suppression, and users’ comfort. Therefore, this paper has proposed a genetic algorithm based temperature-queuing control method for aggregated IAC load control, which could suppress load rebound under the premise of ensuring the DR target and take users’ comfort into account. Firstly, the model of the aggregated IAC load is established by the Monte Carlo method. Then the start and end time of DR are selected as the main solution variables. A genetic algorithm is used as the solving tool. The simulation results show that the proposed strategy shows better performance in suppressing load rebound. In the specific application scenario of adjusting the frequency fluctuation of the microgrid, the results of the case show that this strategy can effectively control the frequency fluctuation of the microgrid. The effectiveness of the strategy is verified.
Multi-Source Information Monitoring Test of Fractured Rock Mass Destruction Characteristics and Sensitivity Analysis of Precursor Phenomena
Authors: Zhang Qinghe , Zheng Tianle , Wang Xiaorui , Fang Zhiyuan
Published: January 2022

The accuracy of the monitoring information is particularly important for exploring fractured rock mass deformation and failure mechanisms and precursor characteristics. Appropriate monitoring methods can not only timely and effectively reflect the failure laws of fractured rock masses but also play an early warning role. To explore more reasonable monitoring methods, uniaxial compression experiments and real-time non-destructive monitoring on prefabricated fractured rock specimens through DIC, AE, and IRT were conducted; the strain field, temperature field, ringing frequency, standard deviation, etc. were analyzed; and correlation between the three methods in the information of audience was explored. The results show the following. (1) The failure evolution process of fractured rock mass can be divided into four stages. DIC can detect the initiation and propagation of cracks near the fractures of the specimen at the earliest stages. (2) The order of occurrence of precursor phenomena in multi-source monitoring information is different, which is vertical strain field > shear strain field > horizontal strain field > temperature field > ringing times. (3) The dispersion degree of standard deviation of each field is obviously different; the infrared temperature field is greater, but the strain field and temperature field show the same trend. (4) There are obvious precursors before the specimen is on the verge of instability; acoustic emission detected two consecutive increases in the cumulative number of ringing before destruction, which means the most obvious precursors. The research results can provide a theoretical basis for the precursory information capture and damage early warning of the fractured rock mass destruction process.
A Flexibility Market Platform for Electricity System Operators Using Blockchain Technology
Authors: Mladenov Valeri , Chobanov Vesselin , Seritan George Calin , Porumb Radu Florin , Enache Bogdan-Adrian , Vita Vasiliki , Stănculescu Marilena , Vu Van Thong , Bargiotas Dimitrios
Published: January 2022

The paper’s main objective is to demonstrate the trading and flexibility of services amongst TSOs, DSOs, and Prosumers in a transparent, secure, and cost-effective manner using Blockchain-based TSO-DSO flexibility marketplace (EFLEX). The aim is to look for ways to help DSOs/TSOs be more flexible and more directly engaged in managing energy flows on the network. EFLEX will streamline the needs of both TSO and DSO on the same platform. Based on the paper’s proposed services, the pilot service demonstration will be carried out in Bulgaria and Romania, and the main focus will be on congestion management, TSO-DSO Coordination, and Marketplace. The proposed objective is achieved by using Blockchain-based smart contracts and distributed ledger technology.
Electrochemically Enhanced Deposition of Scale from Chosen Formation Waters from the Norwegian Continental Shelf
Authors: Emmel Benjamin Udo , Gawel Kamila Maria , Bhuiyan Mohammad Hossain , Torsæter Malin , Edvardsen Laura
Published: January 2022

Reservoir formation waters typically contain scaling ions which can precipitate and form mineral deposits. Such mineral deposition can be accelerated electrochemically, whereby the application of potential between two electrodes results in oxygen reduction and water electrolysis. Both processes change the local pH near the electrodes and affect the surface deposition of pH-sensitive minerals. In the context of the plugging and abandonment of wells, electrochemically enhanced deposition could offer a cost-effective alternative to the established methods that rely on setting cement plugs. In this paper, we tested the scale electro-deposition ability of six different formation waters from selected reservoirs along the Norwegian continental shelf using two experimental setups, one containing CO2 and one without CO2. As the electrochemical deposition of scaling minerals relies on local pH changes near the cathode, geochemical modelling was performed to predict oversaturation with respect to the different mineral phases at different pH values. In a CO2-free environment, the formation waters are mainly oversaturated with portlandite at pH > 12. When CO2 was introduced to the system, the formation waters were oversaturated with calcite. The presence of mineral phases was confirmed by powder X-ray diffraction (XRD) analyses of the mineral deposits obtained in the laboratory experiments. The geochemical-modelling results indicate several oversaturated Mg-bearing minerals (e.g., brucite, dolomite, aragonite) in the formation waters but these, according to XRD results, were absent in the deposits, which is likely due to the significant domination of calcium-scaling ions in the solution. The amount of deposit was found to be proportional to the concentration of calcium present in the formation waters. Formation waters with a high concentration of Ca ions and a high conductivity yielded more precipitate.