bluuebunny/crossref_metadata_2025 huggingface dataset:
Proximal Abduction Ulnar Osteotomy (PAUL): Short- and Long-Term Evaluation in Dogs Presenting Medial Compartment Disease
Authors: Ballester Carolina Oliver , Canet Carme Soler , García José Ignacio Redondo , Salesa Nuria Fernández , Canet Vicente Sifre , Aguado Claudio Iván Serra
Published: February 2022

The aim of the study is to report the results obtained from performing a Proximal Abduction Ulnar Osteotomy (PAUL) technique in a cohort of dogs with medial compartment disease with short- and long-term follow-up, assessing the perception of the owners and describing the long-term complications associated with the technique. This is a clinical prospective study, including dogs diagnosed with medial compartment disease treated with elbow arthroscopy and PAUL between 2013 and 2020. Long-term follow-up data and postoperative complications were registered, and a questionnaire adapted from Fitzpatrick et al. 2009 was collected from owners. Thirty-three elbows in 26 dogs were included. The duration of follow-up ranged from 4 to 61 months (median: 24.76 months). At the end of the study, 73.1% of the owners reported excellent satisfaction and 74.1% of the owners would repeat the surgery in the same circumstances. The owner questionnaire showed a greater capacity to walk and run, without lameness and pain, and climb up and down stairs a year after surgery, being constant at the end of the study. Major postoperative complications were documented in 4/33 elbows (12.12%), including delayed union, implant failure, and osteophytosis of the medial aspect of the coronoid process. In conclusion, elbow arthroscopy and the PAUL technique achieved an evident improvement in the quality of life of patients with great satisfaction for most of the owners.
Associations of Apoptotic and Anti-Apoptotic Factors with Beef Quality, Histochemical Characteristics, and Palatability of Hanwoo Longissimus thoracis Muscle
Authors: Lee Boin , Kim Jae-Yeong , Choi Young-Min
Published: February 2022

This study compared the meat quality, histochemical traits, palatability, and expression levels of apoptotic (cytochrome c and caspases) and anti-apoptotic (small heat shock proteins) factors at 45 min and 24 h post-mortem of Hanwoo Longissimus thoracis muscles in groups categorized by Warner-Bratzler shear force (WBS) values to investigate the association between beef tenderness variation and apoptosis-related molecules. There were no differences in marbling scores, meat quality traits, or histochemical characteristics among the WBS groups (p > 0.05) indicating no significant effect on the tenderness variation in the current study. On the other hand, the low group exhibited higher levels of apoptotic and anti-apoptotic factors (except for αβ-crystallin) at 45 min post-mortem compared to the high WBS group, resulting in higher scores of tenderness attributes (p < 0.05). However, the level of αβ-crystallin at 45 min post-mortem was lower in the low and medium WBS groups compared to the high WBS group (p < 0.0106). At 24 h post-mortem, no significant differences were observed in the expression levels of apoptosis-related factors among the WBS groups (p > 0.05) except for heat shock protein 27 (p < 0.05).
Consumers’ Expectations about Meat from Surgical Castrated or Immunocastrated Male and Female Iberian Pigs
Authors: Font-i-Furnols Maria , Claret Anna , Guerrero Luis , Dalmau Antoni
Published: February 2022

A common practice in Iberian pigs is the castration of both males and females, and it can be carried out surgically or by immunization against gonadotropin-releasing factor (GnRF). The aim of this work was to determine consumers’ overall liking and expectations towards Iberian pork from five different sex types (castrated females, entire females, GnRF-vaccinated females, castrated males and GnRF-vaccinated males), as well as to know the attitudes and beliefs of consumers towards castration and immunocastration. Loins from 83 Iberian pigs were collected and evaluated by 252 consumers in Barcelona and Madrid. Consumers evaluated the five types of meat in three situations: blind condition (tasting the product), expectations (without tasting) and informed condition. Finally, attitudes and beliefs towards castration and immunocastration were also determined. Results distinguished three segments of consumers labeled as “Indifferent”, “Against castration and immunocastration” and “Against immunocastration”. Meat from castrated males had higher overall liking scores in the blind condition. Expectations towards pork depending on its sex type affect consumer-informed acceptability; thus, it is important to consider marketing strategies to avoid or direct the effect of the information provided on the acceptability of the pork.
Dairy Cows’ Udder Pathogens and Occurrence of Virulence Factors in Staphylococci
Authors: Zigo František , Farkašová Zuzana , Výrostková Jana , Regecová Ivana , Ondrašovičová Silvia , Vargová Mária , Sasáková Naďa , Pecka-Kielb Ewa , Bursová Šárka , Kiss David Sandor
Published: February 2022

This study investigated 960 Slovak and Czech spotted cattle from four different conventional (non-organic) dairy herds located in Eastern Slovakia and Czechia during early lactation (14–100 days after calving). Dairy cows were examined clinically; milk from fore-stripping of each udder quarter was subjected to sensory examination and assessed by the California mastitis test (CMT), and laboratory analyses of bacterial pathogens in milk, including virulence factors, were conducted. Positive CMT scores (1–3) for one or more quarters were detected in 271 (28.2%) of the examined animals. Out of 230 infected milk samples, representing 24.0% of all dairy cows, staphylococci (59.1% of positive findings) were the most commonly isolated organisms, followed by E. coli (11.3%), streptococci Str. uberis (9.1%) and Str. agalactiae (3.4%), and enterococci (6.1%). From 136 isolates of S. aureus (38 isolates) and non-aureus staphylococci (NAS; 98 isolates), virulence factors and their resistance to 14 antimicrobials were detected using the disk diffusion method, with PCR detection of the methicillin resistance gene, mecA. An increased incidence of clinical and chronic forms of mastitis has been reported in mastitic cows in which staphylococci, especially S. aureus and NAS (S. chromogenes, S. warneri, and S. xylosus), have been detected and compared to other isolated udder pathogens. From those species, S. aureus and isolates of NAS mentioned above showed multiple virulence factors that are more likely to hydrolyze DNA, hemolysis, produce gelatinase and biofilm, and have multi-drug resistance as compared to other less virulent staphylococci. Generally, the isolated staphylococci showed 77.2% resistance to one or more antimicrobials, in particular to aminoglycosides, β-lactams, macrolides, or cephalosporins. Isolates that showed the ability to form a biofilm were more resistant to more than one antimicrobial than isolates without biofilm production. Multi-drug resistance to three or more antimicrobial classes was recorded in 16 isolates (11.7%), and the presence of the mecA gene was also confirmed in two isolates of S. aureus and two species of NAS.
Symmetry-Breaking Stabilities in Carapace Curvature on Testudo (Reptilia, Testudinidae)
Authors: Parés-Casanova Pere M. , Soler Joaquim , Buisán Tania , Martínez-Silvestre Albert
Published: February 2022

The aim of this research was to contribute to the study of the doming geometry of Testudo carapace as an unstable point of equilibrium when animals are overturned. We performed this research using geometric morphometric using a sample of 64 Testudo individuals belonging to different species (T. hermannin = 30, T. graecan = 3, T. marginata n = 13 and T. horsfieldii n = 18), sexes and ages. A set of four sagittal landmarks (discrete homologous points) and 15 pairs of semi-landmarks, on the frontal doming of the carapace, were digitized on individual carapace pictures. Significative fluctuating asymmetry was detected, defined as small, completely random departures from bilateral symmetry, but much less than directional asymmetry, which appeared highly significative. Anti-symmetry did not appear. Carapace asymmetry was dominated by a clear right directionality. A possible biological speculation could be that this asymmetry more that easing the self-righting potential (“kinematic instability”, understood as the ability to self-right without effort), makes stable ventral turning difficult (“static stability”, understood as the ability to resist passively turning the body produced by destabilizing forces). This asymmetry is present among both sexes but more marked among males. An explanation for this sexually differentiated pattern could be the higher locomotion and the fight for mating in males, making them consequently more prone to losing their balance and falling on their back. These data may be useful in studying adaptative traits in Testudo species as well as establishing a seminal base for future studies. This research is the first attempt to explore a suitable method to assess doming asymmetry which could be useful in future, more extensive investigations, on a larger interspecific sample.
Applying Bipartite Network Analysis and Ordination Technique to Evaluate Long-Term Data from Veterinary–Sanitary Examination of Slaughtered Pigs
Authors: Majewski Michał , Dylewski Łukasz , Grabowski Sebastian , Racewicz Przemysław , Tryjanowski Piotr
Published: February 2022

Animal and meat inspections in abattoirs are important in the surveillance of zoonotic diseases. Veterinary inspections in abattoirs can provide useful data for the management of health and welfare issues of humans and animals. Using the network analysis and ordination technique, in this study, we analyzed the data from 11 years of veterinary inspections in pig slaughterhouses from 16 regions in Poland. Based on the huge data set of 80,187,639 cases of diseases and welfare issues of pigs, the most frequent livestock diseases were identified to be abscesses, soiling, faecal or other contaminations, and congestions, which together accounted for 77.6% of the total condemnations. Spatial and temporal differences in swine diseases between the Polish regions were recognized using the above-mentioned statistical approaches. Moreover, with the use of a quite novel method, not used yet in preventive veterinary medicine, called a heatmap, the most problematic disease and welfare issues in each region in Poland were identified. The use of statistical approaches such as network analysis and ordination technique allow for identification of the health and welfare issues in slaughterhouses when dealing with long-term inspection data based on a very large number of cases, and then have to be adopted in current veterinary medicine.
Novel Plant-Based Metabolites as Disinfectants against Acanthamoeba castellanii
Authors: Siddiqui Ruqaiyyah , Akbar Noor , Khatoon Bushra , Kawish Muhammad , Ali Muhammad Shaiq , Shah Muhammad Raza , Khan Naveed Ahmed
Published: February 2022

Due to global warming, coupled with global water shortages and the reliance of the public on household water tanks, especially in developing countries, it is anticipated that infections caused by free-living amoebae such as Acanthamoeba will rise. Thus, the development of novel disinfectant(s) which can target pathogenic free-living amoebae effectively is warranted. Herein, we extracted and isolated several plant-based secondary metabolites as novel disinfectants for use against pathogenic Acanthamoeba. The identity of the compounds was confirmed by nuclear magnetic resonance and tested for antiamoebic activities against clinical isolate of A. castellanii, belonging to the T4 genotype. Amoebicidal assays revealed that the compounds tested showed antiamoebic properties. Betulinic acid and betulin exhibited parasite killing of more than 65%. When tested against the cyst stage, betulinic acid, betulin, and vanillic acid inhibited both encystation and excystation processes. Furthermore, the plant-based metabolites significantly inhibited the binding capability of A. castellanii to host cells. Finally, most of the tested compounds displayed minimal cytotoxic activities against human cells and noticeably perturbed amoeba-mediated host cell cytotoxicity. Notably, both alkaloid and betulinic acid showed 20% cytotoxic effects, whereas betulin and lupeol had cytotoxic effects of 24% and 30%, respectively. Overall, our findings indicate that plant-based natural compounds demonstrate anti-Acanthamoebic properties, and they have potential candidates for water disinfectants or contact lens disinfecting solutions, as well as possible therapeutic drugs against Acanthamoeba infections.
HNO Protects the Myocardium against Reperfusion Injury, Inhibiting the mPTP Opening via PKCε Activation
Authors: Mancardi Daniele , Pagliaro Pasquale , Ridnour Lisa A. , Tocchetti Carlo G. , Miranda Katrina , Juhaszova Magdalena , Sollott Steven J. , Wink David A. , Paolocci Nazareno
Published: February 2022

Donors of nitroxyl (HNO), the one electron-reduction product of nitric oxide (NO.), positively modulate cardiac contractility/relaxation while limiting ischemia-reperfusion (I/R) injury. The mechanisms underpinning HNO anti-ischemic effects remain poorly understood. Using isolated perfused rat hearts subjected to 30 min global ischemia/1 or 2 h reperfusion, here we tested whether, in analogy to NO., HNO protection requires PKCε translocation to mitochondria and KATP channels activation. To this end, we compared the benefits afforded by ischemic preconditioning (IPC; 3 cycles of I/R) with those eventually granted by the NO. donor, diethylamine/NO, DEA/NO, and two chemically unrelated HNO donors: Angeli’s salt (AS, a prototypic donor) and isopropylamine/NO (IPA/NO, a new HNO releaser). All donors were given for 19 min before I/R injury. In control I/R hearts (1 h reperfusion), infarct size (IS) measured via tetrazolium salt staining was 66 ± 5.5% of the area at risk. Both AS and IPA/NO were as effective as IPC in reducing IS [30.7 ± 2.2 (AS), 31 ± 2.9 (IPA/NO), and 31 ± 0.8 (IPC), respectively)], whereas DEA/NO was significantly less so (36.2 ± 2.6%, p < 0.001 vs. AS, IPA/NO, or IPC). IPA/NO protection was still present after 120 min of reperfusion, and the co-infusion with the PKCε inhibitor (PKCV1-2500 nM) prevented it (IS = 30 ± 0.5 vs. 61 ± 1.8% with IPA/NO alone, p < 0.01). Irrespective of the donor, HNO anti-ischemic effects were insensitive to the KATP channel inhibitor, 5-OH decanoate (5HD, 100 μM), that, in contrast, abrogated DEA/NO protection. Finally, both HNO donors markedly enhanced the mitochondrial permeability transition pore (mPTP) ROS threshold over control levels (≅35–40%), an action again insensitive to 5HD. Our study shows that HNO donors inhibit mPTP opening, thus limiting myocyte loss at reperfusion, a beneficial effect that requires PKCε translocation to the mitochondria but not mitochondrial K+ channels activation.
Comparison of Various Solvent Extracts and Major Bioactive Components from Unsalt-Fried and Salt-Fried Rhizomes of Anemarrhena asphodeloides for Antioxidant, Anti-α-Glucosidase, and Anti-Acetylcholinesterase Activities
Authors: Chu Yi-Cheng , Yang Chang-Syun , Cheng Ming-Jen , Fu Shu-Ling , Chen Jih-Jung
Published: February 2022

The rhizome of Anemarrhena asphodeloides Bunge (AA, family Liliaceae) is a famous and frequently used herbal drug in the traditional medicine of Northeast Asia, under vernacular name “zhimu”. A. asphodeloides has been used as an anti-inflammatory, antipyretic, anti-platelet aggregation, anti-depressant, and anti-diabetic agent in traditional Chinese medicine. We examined the antioxidant, anti-acetylcholinesterase (AChE), and anti-α-glucosidase activities of various solvent extracts and the main bioactive compounds from the rhizome of A. asphodeloides. Acetone extract exhibited comparatively high antioxidant activities by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric-reducing antioxidant power (FRAP) assays. A water extract exhibited relatively strong antioxidant activity by superoxide radical scavenging test. Furthermore, dichloromethane, chloroform, and n-hexane extracts showed significant anti-α-glucosidase activities. Finally, ethanol and dichloromethane extracts exhibited relatively strong AChE inhibitory activity. HPLC analysis was used to examine and compare various solvent extracts for their compositions of isolates. We isolated four major chemical constituents and analyzed their antioxidant, anti-α-glucosidase, and AChE inhibitory activities. The bioactivity assays showed that mangiferin displayed the most potential antioxidant activities via FRAP, ABTS, DPPH, and superoxide assays and also exhibited the most effective anti-AChE and anti-α-glucosidase activities among all the isolates. The present study suggests that A. asphodeloides and its active extracts and components are worth further investigation and might be expected to develop as a candidate for the treatment or prevention of oxidative stress-related diseases, AChE inhibition, and hyperglycemia.
Joint Hamming Coding for High Capacity Lossless Image Encryption and Embedding Scheme
Authors: Chen Yi-Hui , Lin Pei-Yu , Wu Hsin-Pei , Chen Shih-Hsin
Published: February 2022

Encryption is a widely used solution to prevent privacy leakage and illegal spread when sensitive images are uploaded to cloud storage. Hiding technology also allows confidential data to be embedded into encrypted images for secret communication. As image accuracy without distortion is essential within certain fields (such as medicine and the military), sensitive images must be completely decrypted back into the original images. However, an encrypted image is a noise-like pattern that is meaningless to a user; thus, it is difficult for a user to find the accurate image they desire. Take keywords as search indexes and embed them in encrypted images for encrypted image retrieval as an example. This idea has been extended by Chen and Line’s scheme to achieve higher capacity with reversibility. The proposed scheme adjusts the coding results according to smooth and complex images to increase its hiding capacity. In addition, two thresholds are designed to adjust the predicted pixel value to be close to the original one. Experiments show that compared with the other schemes, the proposed method achieves superior results. In addition, a hidden encrypted image can be extracted from the cover image. Afterward, the hidden secrets can be completely extracted, and sensitive images can also be perfectly restored.
Simulation of the Influence of Wing Angle Blades on the Performance of Counter-Rotating Axial Fan
Authors: Gao Guijun , You Qingshan , Kou Ziming , Zhang Xin , Gao Xinqi
Published: February 2022

We took the mining counter-rotating fan FBD No.8.0 as the research object, used orthogonal test and numerical simulation to study the influence of wing angle blade on fan performance, and simulated and analyzed its aerodynamic noise. The results show that the pressure distribution of the optimal blade angle blade fan on the pressure surface of the secondary blade is stronger than that of the prototype blade, and the maximum pressure at the blade height of 25%, 50%, and 75% is increased by 2.3%, 9.3%, and 8.1%, respectively, than original blade. Compared with the prototype blade; wing angle blades can effectively reduce the generation of shedding vortices at the trailing edge of the blade, and reduce the strength of shedding vortices, so that the entropy production of the optimal wing angle blade fan is 1.55% lower than that of the prototype fan. Compared with the prototype fan, the full pressure and efficiency of the angle blade fan under the rated flow have increased by 7.24% and 1.76%, and the average increase of 11.32% and 3.88%, respectively, under the full flow condition. Compared with the prototype fan, the maximum sound power of the wing blade fan in the first and second blade trailing edge regions is reduced by 0.17% and 1.62%, respectively.
A THz Passive Image Generation Method Based on Generative Adversarial Networks
Authors: Yang Guan , Li Chao , Liu Xiaojun , Fang Guangyou
Published: February 2022

A terahertz (THz) passive imager with automatic target detection is an effective solution in the field of security inspection. The high-quality training datasets always play a key role in the high-precision target detection applications. However, due to the difficulty of passive image data acquisition and the lack of public dataset resources, the high-quality training datasets are often insufficient. The generative adversarial network (GAN) is an effective method for data augmentation. To enrich the dataset with the generated images, it is necessary to ensure that the generated images have high quality, good diversity, and correct category information. In this paper, a GAN-based generation model is proposed to generate terahertz passive images. By applying different residual connection structures in the generator and discriminator, the models have strong feature extracting ability. Additionally, the Wasserstein loss function with gradient penalty is used to maintain training stability. The self-developed 0.2 THz band passive imager is used to carry out imaging experiments, and the imaging results are collected as a dataset to verify the proposed method. Finally, a quality evaluation method suitable for THz passive image generation task is proposed, and classification tests are performed on the generated images. The results show that the proposed method can provide high-quality images as supplementary.
Long-Range Correlations and Natural Time Series Analyses from Acoustic Emission Signals
Authors: Friedrich Leandro Ferreira , Cezar Édiblu Silva , Colpo Angélica Bordin , Tanzi Boris Nahuel Rojo , Sobczyk Mario , Lacidogna Giuseppe , Niccolini Gianni , Kosteski Luis Eduardo , Iturrioz Ignacio
Published: February 2022

This work focuses on analyzing acoustic emission (AE) signals as a means to predict failure in structures. There are two main approaches that are considered: (i) long-range correlation analysis using both the Hurst (H) and the detrended fluctuation analysis (DFA) exponents, and (ii) natural time domain (NT) analysis. These methodologies are applied to the data that were collected from two application examples: a glass fiber-reinforced polymeric plate and a spaghetti bridge model, where both structures were subjected to increasing loads until collapse. A traditional (AE) signal analysis was also performed to reference the study of the other methods. The results indicate that the proposed methods yield reliable indication of failure in the studied structures.
White Grape Pomace Valorization for Remediating Purposes
Authors: Gavrilaș Simona , Calinovici Ioan , Chiș Sabin , Ursachi Claudiu-Ștefan , Raț Mirabela , Munteanu Florentina-Daniela
Published: February 2022

The present investigation focuses on a possible alternative to reduce grape pomace’s negative impact on the environment by exploiting its capacities for wastewater remediation. Therefore, three directions are followed: (1) the characterization of white grape byproducts’ antioxidant and antiradical properties; (2) the determination of organophosphorus compound concentration that might be present in the samples; and (3) the evaluation of the possible use for wastewater remediation. The grape pomace was used for obtaining different extracts considering different extraction conditions. The hydroalcoholic extracts have an increased amount of total phenolic content. The lyophilized hydroalcoholic extract showed high total phenolic content (159.52 mg/100 g) and low organophosphorus content (0.257 ± 0.015 nmol). The lowest DPPH antiradical-scavenging activities were obtained for the extracts in ethanol obtained from refrigerated pomace (0.055) and for dried pomace (0.045).
Spatio-Temporal Variability in Hydroclimate over the Upper Yangtze River Basin, China
Authors: Yang Ruting , Xing Bing
Published: February 2022

As global warming produces dramatic climate changes, water management is facing increasingly serious challenges. Given to the process of climate change and its complex effects on watershed hydrology, this paper investigates the spatial and temporal variation characteristics of major climatic factors (i.e., precipitation and temperature) over the upper Yangtze River basin (UYRB), China. The statistical analyses are based on annual and seasonal scales during 1951–2020 with a recorded period of seven decades. The Mann–Kendall nonparametric test and R/S analysis are used to record the temporal trends (past and future) of climate variables; the Pettitt test, standard normal homogeneity test and Buishand test are used to detect the homogeneity in climate series. The sensitivities of the streamflow to climatic parameters are assessed at the watershed scale, especially considering the Three Gorges Dam’s (TGD) effect on changing runoff. The results of the study indicate that the annual precipitation of 29 out of 34 series indicate homogeneity, while 31 out of 34 annual mean temperature series show heterogeneity, with jump points around 1997 in the mean temperature of 20 sites. Detectable changes in precipitation were not observed during 1951–2020; however, the temperature increased significantly in the whole basin on annual and seasonal scales, except for several stations in the eastern part. The magnitude of increase in air temperature in high altitudes (Tibet Plateau) is higher than that in low altitudes (Sichuan Plain) over the last seven decades, and future temperatures continue to sharply increase in high altitudes. The TGD plays an important role in explaining the seasonal variations in streamflow at Yichang station, with streamflow experiencing a sharp increase in winter and spring (dry season) and a decrease in summer and autumn (rainy season) compared to the pre-TGD period. The streamflow variation at an annual scale is mainly regulated by climate fluctuation (variation in precipitation). During the last seven decades, increasing air temperature and decreases in rainfall and runoff signify reduced water resources availability, and the climate tends to be warmer and drier over the basin. The sensitivity of the streamflow to watershed precipitation is higher than that to temperature, with variation in annual rainfall explaining 71% of annual runoff variability.
High-Aspect-Ratio Microfluidic Channel with Parallelogram Cross-Section for Monodisperse Droplet Generation
Authors: Ji Hyeonyeong , Lee Jaehun , Park Jaewon , Kim Jungwoo , Kim Hyun Soo , Cho Younghak
Published: February 2022

Droplet-based microfluidics has been widely used as a potent high-throughput platform due to various advantages, such as a small volume of reagent consumption, massive production of droplets, fast reaction time, and independent control of each droplet. Therefore, droplet microfluidic systems demand the reliable generation of droplets with precise and effective control over their size and distribution, which is critically important for various applications in the fields of chemical analysis, material synthesis, lab-on-a-chip, cell research, diagnostic test, and so on. In this study, we propose a microfluidic device with a high-aspect-ratio (HAR) channel, which has a parallelogram cross-section, for generating monodisperse droplets. The HAR channel was fabricated using simple and cheap MEMS processes, such as photolithography, anisotropic wet etching, and PDMS molding, without expensive equipment. In addition, the parallelogram cross-section channel structure, regarded as a difficult shape to implement in previous fabrication methods, was easily formed by the self-alignment between the silicon channel and the PDMS mold, both of which were created from a single crystal silicon through an anisotropic etching process. We investigated the effects of the cross-sectional shape (parallelogram vs. rectangle) and height-to-width ratio of microfluidic channels on the size and uniformity of generated droplets. Using the developed HAR channel with the parallelogram cross-section, we successfully obtained smaller monodisperse droplets for a wider range of flow rates, compared with a previously reported HAR channel with a rectangular cross-section.
Enhanced Blood Plasma Extraction Utilising Viscoelastic Effects in a Serpentine Microchannel
Authors: Dai Yuchen , Cha Haotian , Simmonds Michael J. , Fallahi Hedieh , An Hongjie , Ta Hang T. , Nguyen Nam-Trung , Zhang Jun , McNamee Antony P.
Published: February 2022

Plasma extraction from blood is essential for diagnosis of many diseases. The critical process of plasma extraction requires removal of blood cells from whole blood. Fluid viscoelasticity promotes cell migration towards the central axis of flow due to differences in normal stress and physical properties of cells. We investigated the effects of altering fluid viscoelasticity on blood plasma extraction in a serpentine microchannel. Poly (ethylene oxide) (PEO) was dissolved into blood to increase its viscoelasticity. The influences of PEO concentration, blood dilution, and flow rate on the performance of cell focusing were examined. We found that focusing performance can be significantly enhanced by adding PEO into blood. The optimal PEO concentration ranged from 100 to 200 ppm with respect to effective blood cell focusing. An optimal flow rate from 1 to 15 µL/min was determined, at least for our experimental setup. Given less than 1% haemolysis was detected at the outlets in all experimental combinations, the proposed microfluidic methodology appears suitable for applications sensitive to haemocompatibility.
A Social Business Model of Early Intervention and Rehabilitation for People with Disability in Rural Bangladesh
Authors: Al Imam Mahmudul Hassan , Das Manik Chandra , Jahan Israt , Muhit Mohammad , Akbar Delwar , Badawi Nadia , Khandaker Gulam
Published: February 2022

Background: Despite the high burden of childhood disability in low-and middle-income countries (LMICs), the opportunity for early intervention and rehabilitation is very limited. Studies have found that community-based rehabilitation service is effective for children with cerebral palsy (CP); however, such services are not readily available in LMICs, and services run by non-profit organisations on external funding are often not sustainable. In this study, we report the lesson learnt in establishing a social business model of early intervention and rehabilitation services for children with CP and adults with disabilities in a rural subdistrict of Bangladesh. Methods: Case study of a rural early intervention and rehabilitation centre (i.e., the model centre) implemented between May 2018 and September 2019. An economic evaluation incorporating gross margin analysis along with descriptive statistics was performed to assess the social business potentials of the model centre. Results: The establishment of this model centre cost ~5955 USD with an average monthly running cost of ~994 USD. During the 17 months study period, 7038 therapy sessions (average eight sessions per patient) were offered to 862 patients with musculoskeletal and neurological disorders. The most common clinical presentations were low back pain (35.6%; n = 307). Six percent (n = 52) of the attendees were children with CP (mean (SD) age 6.3 (4.0) years; 35.7% (n = 19) were female), who received 1392 sessions, on average 27 sessions per child. The centre reached the break-even point at the 13th month and remained profitable for the next 4 months of the study period. An average session fee of 2.2 USD resulted in a gross margin of -1458 USD and 1940 USD in 2018 and 2019, respectively. Revenue to cost ratios for the 2 years were 0.27:1 and 0.51:1 while average rates of return were −41.4% and 10.1%, respectively. Sensitivity analysis revealed that session numbers including 5000, 6000, 7000, 8000, 9000, and 10,000 were required to break even at the session fees of 3.0, 2.50, 2.0, 2.0, 1.5, and 1.5 USD, respectively. Conclusion: Our social business model of an early intervention and rehabilitation service provides evidence of enhancing access to services for children with CP as well as adults with disabilities while ensuring the sustainability of the services in rural Bangladesh.
Psychopathology and Integrity of the Superior Longitudinal Fasciculus in Deficit and Nondeficit Schizophrenia
Authors: Podwalski Piotr , Tyburski Ernest , Szczygieł Krzysztof , Rudkowski Krzysztof , Waszczuk Katarzyna , Andrusewicz Wojciech , Kucharska-Mazur Jolanta , Michalczyk Anna , Mak Monika , Cyranka Katarzyna , Misiak Błażej , Sagan Leszek , Samochowiec Jerzy
Published: February 2022

The superior longitudinal fasciculus (SLF) is a white matter bundle that connects the frontal areas with the parietal areas. As part of the visuospatial attentional network, it may be involved in the development of schizophrenia. Deficit syndrome (DS) is characterized by primary and enduring negative symptoms. The present study assessed SLF integrity in DS and nondeficit schizophrenia (NDS) patients and examined possible relationships between it and psychopathology. Twenty-six DS patients, 42 NDS patients, and 36 healthy controls (HC) underwent psychiatric evaluation and diffusion tensor imaging (DTI). After post-processing, fractional anisotropy (FA) values within the SLF were analyzed. Psychopathology was assessed with the Positive and Negative Syndrome Scale, Brief Negative Symptom Scale, and Self-evaluation of Negative Symptoms. The PANSS proxy for the deficit syndrome was used to diagnose DS. NDS patients had lower FA values than HC. DS patients had greater negative symptoms than NDS patients. After differentiating clinical groups and HC, we found no significant correlations between DTI measures and psychopathological dimensions. These results suggest that changes in SLF integrity are related to schizophrenia, and frontoparietal dysconnection plays a role in its etiopathogenesis. We confirmed that DS patients have greater negative psychopathology than NDS patients. These results are preliminary; further studies are needed.
“The Best Laid Plans”: Do Individual Differences in Planfulness Moderate Effects of Implementation Intention Interventions?
Authors: Sas Sabryna V. , Hamilton Kyra , Hagger Martin S.
Published: February 2022

While there is good evidence supporting the positive effect of planning strategies like implementation intentions on the relationship between intention and behavior, there is less evidence on the moderating role of individual differences in planning capacity on this effect. This study aimed to examine the role of individual differences in planfulness on the effect of planning strategies on the intention–behavior gap. Specifically, this study investigated the influence of planfulness on the effectiveness of implementation intentions on goal-directed behavior using an experimental design. Undergraduate university students (N = 142) with high and low levels of planfulness based on a priori scores on a planfulness measure were randomized to either a planning (implementation intention) or familiarization (control) condition prior to completing a computerized go no-go task. We predicted that individuals reporting low levels of planfulness would be more effective in executing goal-directed behavior when prompted to form an implementation intention compared to individuals who do not receive a prompt. Additionally, we predicted that individuals reporting high planfulness would be equally effective in enacting goal-directed behaviour regardless of whether they formed an implementation intention. The results revealed no main or interaction effects of implementation intention manipulation and planfulness on task reaction times. The current results do not provide support for the moderating effect of planfulness on the implementation effect. The findings of this study were inconsistent with previous literature. This research has implications for the effectiveness of implementation intentions, as well as opportunities for further replication in a novel research area.
Critical Experiments for Structural Members of Micro Image Strain Sensing Sensor Based on Smartphone and Microscope
Authors: Chen Xixian , Zhang Lixiao , Xie Botao , Zhou Guangyi , Zhao Xuefeng
Published: February 2022

Strain is one of the key concerns of structural health monitoring (SHM), and effective measurement of structural strain is very important for structural health condition assessment. The purpose of this paper is to investigate the application of the micro image strain sensing (MISS) sensor based on smartphone and microscope for measuring strain parameters of structural members. Firstly, metal tensile tests were performed to verify the effectiveness of the MISS sensor, and the strain results measured by the fiber Bragg grating (FBG) sensor and the MISS sensor were compared. The results showed that the mean error between the MISS and FBG sensor measured results is 7.1 με in the metal tensile test. Then, a concrete beam bending test was carried out; the mean relative error of strain values between the FBG sensor and the MISS sensor is 2.5%. Overall, the strain measurements of the MISS sensor matched well with those from the FBG sensor in each experiment. This study validates the reliability and accuracy of the MISS sensor for strain detection and the MISS sensors can play a more powerful role in SHM due to their robustness, simplicity, and low cost.
Metabolic Syndrome and Physical Inactivity May Be Shared Etiological Agents of Prostate Cancer and Coronary Heart Diseases
Authors: Cicione Antonio , Brassetti Aldo , Lombardo Riccardo , Franco Antonio , Turchi Beatrice , D’Annunzio Simone , Nacchia Antonio , Tubaro Andrea , Simone Giuseppe , De Nunzio Cosimo
Published: February 2022

As metabolic syndrome (MetS) and a sedentary lifestyle have been associated with an increased risk of developing both prostate cancer (PCa) and cardiovascular diseases (CVDs), the 2 conditions may share a common etiology. We aimed at investigating the association between CVDs and PCa. A retrospective analysis was performed. Our dataset on patients undergone systematic prostate biopsy was searched for histopathologic and clinical data. The physical activity (PA) scale for the elderly (PASE) was collected. Coronary heart diseases (CHDs) were recorded. Prognostic Grade Group ≥3 tumors were defined as high-grade (HG). The association between MetS, PA, CHDs and PCa was assessed using logistic regression analyses. Data on 955 patients were collected; 209 (22%) presented with MetS, 79 (8%) with CHDs. PCa was diagnosed in 395 (41.3%) men and 60% (n = 238) presented with an high-grade tumor. CHDs were more common among PCa-patients (9.4% vs. 7.5%; p = 0.302) but the difference was not statistically significant. No difference was observed between low- and high-grade subgroups (9.5% vs. 9.2%; p = 0.874). PASE independently predicted PCa diagnosis (OR: 0.287; p = 0.001) and HG-PCa (OR: 0.165; p = 0.001). MetS was an independent predictor of HG-PCa only (OR: 1.50; 95% CI: 1.100–2.560; p = 0.023). CHDs were not associated with tumor diagnosis and aggressiveness.
Current Status of CRISPR/Cas9 Application in Clinical Cancer Research: Opportunities and Challenges
Authors: Rafii Saeed , Tashkandi Emad , Bukhari Nedal , Al-Shamsi Humaid O.
Published: February 2022

Cancer is considered by not only multiple genetic but also epigenetic amendments that drive malignant cell propagation and consult chemo-resistance. The ability to correct or ablate such mutations holds enormous promise for battling cancer. Recently, because of its great efficiency and feasibility, the CRISPR-Cas9 advanced genome editing technique has been extensively considered for therapeutic investigations of cancers. Several studies have used the CRISPR-Cas9 technique for editing cancer cell genomic DNA in cells and animal cancer models and have shown therapeutic potential in intensifying anti-cancer protocols. Moreover, CRISPR-Cas9 may be used to correct oncogenic mutations, discover anticancer drugs, and engineer immune cells and oncolytic viruses for immunotherapeutic treatment of cancer. We herein discuss the challenges and opportunities for translating therapeutic methods with CRISPR-Cas9 for clinical use and suggest potential directions of the CRISPR-Cas9 system for future cancer therapy.
Minimally Invasive Endoscopic and Surgical Management of Rectal Neoplasia
Authors: Al Ghamdi Sarah , Leeds Ira , Fang Sandy , Ngamruengphong Saowanee
Published: February 2022

Rectal cancer demonstrates a characteristic natural history in which benign rectal neoplasia precedes malignancy. The worldwide burden of rectal cancer is significant, with rectal cancer accounting for one-third of colorectal cancer cases annually. The importance of early detection and successful management is essential in decreasing its clinical burden. Minimally invasive treatment of rectal neoplasia has evolved over the past several decades, which has led to reduced local recurrence rates and improved survival outcomes. The approach to diagnosis, staging, and selection of appropriate treatment modalities is a multidisciplinary effort combining interventional endoscopy, surgery, and radiology tools. This review examines the currently available minimally invasive endoscopic and surgical management options of rectal neoplasia.
Partial Methane Oxidation in Fuel Cell-Type Reactors for Co-Generation of Energy and Chemicals: A Short Review
Authors: de Souza Rodrigo F. B. , Florio Daniel Z. , Antolini Ermete , Neto Almir O.
Published: February 2022

The conversion of methane into chemicals is of interest to achieve a decarbonized future. Fuel cells are electrochemical devices commonly used to obtain electrical energy but can be utilized either for chemicals’ production or both energy and chemicals cogeneration. In this work, the partial oxidation of methane in fuel cells for electricity generation and valuable chemicals production at the same time is reviewed. For this purpose, we compile different types of methane-fed fuel cells, both low- and high-temperature fuel cells. Despite the fact that few studies have been conducted on this subject, promising results are driving the development of fuel cells that use methane as a fuel source for the cogeneration of power and valuable chemicals.
Evaluation of Au/ZrO2 Catalysts Prepared via Postsynthesis Methods in CO2 Hydrogenation to Methanol
Authors: Sagar Tatiparthi Vikram , Zavašnik Janez , Finšgar Matjaž , Novak Tušar Nataša , Pintar Albin
Published: February 2022

Au nanoparticles supported on ZrO2 enhance its surface acidic/basic properties to produce a high yield of methanol via the hydrogenation of CO2. Amorphous ZrO2-supported 0.5–1 wt.% Au catalysts were synthesized by two methods, namely deposition precipitation (DP) and impregnation (IMP), characterized by a variety of techniques, and evaluated in the process of CO2 hydrogenation to methanol. The DP-method catalysts were highly advantageous over the IMP-method catalyst. The DP method delivered samples with a large surface area, along with the control of the Au particle size. The strength and number of acidic and basic sites was enhanced on the catalyst surface. These surface changes attributed to the DP method greatly improved the catalytic activity when compared to the IMP method. The variations in the surface sites due to different preparation methods exhibited a huge impact on the formation of important intermediates (formate, dioxymethylene and methoxy) and their rapid hydrogenation to methanol via the formate route, as revealed by means of in situ DRIFTS (diffuse reflectance infrared Fourier transform spectroscopy) analysis. Finally, the rate of formation of methanol was enhanced by the increased synergy between the metal and the support.
The Function of Glial Cells in the Neuroinflammatory and Neuroimmunological Responses
Authors: Afridi Ruqayya , Tsuda Makoto , Ryu Hoon , Suk Kyoungho
Published: February 2022

The historical concept of glia just as the glue of brain tissue has been challenged by the accumulation of concrete evidence showing the multifunctional role of these cells during development and in the adult brain [...]
Treatment of Experimental Autoimmune Encephalomyelitis with an Inhibitor of Phosphodiesterase-8 (PDE8)
Authors: Basole Chaitali P. , Nguyen Rebecca K. , Lamothe Katie , Billis Puja , Fujiwara Mai , Vang Amanda G. , Clark Robert B. , Epstein Paul M. , Brocke Stefan
Published: February 2022

After decades of development, inhibitors targeting cyclic nucleotide phosphodiesterases (PDEs) expressed in leukocytes have entered clinical practice for the treatment of inflammatory disorders, with three PDE4 inhibitors being in clinical use as therapeutics for psoriasis, psoriatic arthritis, chronic obstructive pulmonary disease and atopic dermatitis. In contrast, the PDE8 family that is upregulated in pro-inflammatory T cells is a largely unexplored therapeutic target. We have previously demonstrated a role for the PDE8A-Raf-1 kinase complex in the regulation of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) activated CD4+ effector T cell adhesion and locomotion by a mechanism that differs from PDE4 activity. In this study, we explored the in vivo treatment of experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis (MS) induced in mice immunized with MOG using the PDE8-selective inhibitor PF-04957325. For treatment in vivo, mice with EAE were either subcutaneously (s.c.) injected three times daily (10 mg/kg/dose), or were implanted subcutaneously with Alzet mini-osmotic pumps to deliver the PDE8 inhibitor (15.5 mg/kg/day). The mice were scored daily for clinical signs of paresis and paralysis which were characteristic of EAE. We observed the suppression of the clinical signs of EAE and a reduction of inflammatory lesion formation in the CNS by histopathological analysis through the determination of the numbers of mononuclear cells isolated from the spinal cord of mice with EAE. The PDE8 inhibitor treatment reduces the accumulation of both encephalitogenic Th1 and Th17 T cells in the CNS. Our study demonstrates the efficacy of targeting PDE8 as a treatment of autoimmune inflammation in vivo by reducing the inflammatory lesion load.
Subacromial Bursa: A Neglected Tissue Is Gaining More and More Attention in Clinical and Experimental Research
Authors: Klatte-Schulz Franka , Thiele Kathi , Scheibel Markus , Duda Georg N. , Wildemann Britt
Published: February 2022

The subacromial bursa has long been demolded as friction-reducing tissue, which is often linked to shoulder pain and, therefore, partially removed during shoulder surgery. Currently, the discovery of the stem cell potential of resident bursa-derived cells shed a new light on the subacromial bursa. In the meanwhile, this neglected tissue is gaining more attention as to how it can augment the regenerative properties of adjacent tissues such as rotator cuff tendons. Specifically, the tight fibrovascular network, a high growth factor content, and the large progenitor potential of bursa-derived cells could complement the deficits that a nearby rotator cuff injury might experience due to the fact of its low endogenous regeneration potential. This review deals with the question of whether bursal inflammation is only a pain generator or could also be an initiator of healing. Furthermore, several experimental models highlight potential therapeutic targets to overcome bursal inflammation and, thus, pain. More evidence is needed to fully elucidate a direct interplay between subacromial bursa and rotator cuff tendons. Increasing attention to tendon repair will help to guide future research and answer open questions such that novel treatment strategies could harvest the subacromial bursa’s potential to support healing of nearby rotator cuff injuries.