bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
Authors: Osaki Tomohiro , Yokoe Inoru , Sunden Yuji , Ota Urara , Ichikawa Tomoki , Imazato Hideo , Ishii Takuya , Takahashi Kiwamu , Ishizuka Masahiro , Tanaka Tohru , Li Liming , Yamashita Masamichi , Murahata Yusuke , Tsuka Takeshi , Azuma Kazuo , Ito Norihiko , Imagawa Tomohiro , Okamoto Yoshiharu
Published: April 2019

5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/−chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine.
Few-Layer Graphene from Mechanical Exfoliation of Graphite-Based Materials: Structure-Dependent Characteristics
Authors: Pirzado Azhar A. , Le Normand François , Romero Thierry , Paszkiewicz Sandra , Papaefthimiou Vasiliki , Ihiawakrim Dris , Janowska Izabela
Published: April 2019

We present a high-scale method to produce few-layer graphene (FLG) based on the mechanical exfoliation of graphite and compare the obtained FLG with the one reported earlier arising from pencil lead ablation. Several elements were modified and improved in the new approach. The purification and the ablation set-up were simplified, and the morphology of the FLG was modified and improved in view of some applications. The morphology-dependent properties of FLGs, lead-FLG, and graphite-FLG as conductive layers and in nanocomposites were investigated. The newly obtained FLG had a higher aspect ratio (high lateral size vs thickness/higher 2D aspect), which is reflected by enhanced transparency–conductivity features of the layer (film) and elongation-at-break behavior of the polymer composites. On the contrary, the nanocomposite containing lead-FLG showed, for instance, excellent gas barrier properties due to the multi-step structure of the lead-FLG flakes. Such structure exhibited less 2D and more 3D character, which can be highly suitable for applications where the presence of active/reactive edges is beneficial, e.g., in catalysis or supercapacitors’ electrodes. Nuclear reaction analysis was employed to investigate the morphology of graphite-FLG film.
Evaluation of a Neonatal Resuscitation Curriculum in Liberia
Authors: Chang Mary P. , Walters Camila B. , Tsai Carmelle , Aksamit Deborah , Kateh Francis , Sampson John
Published: April 2019

Neonatal mortality in Africa is among the highest in the world. In Liberia, providers face significant challenges due to lack of resources, and providers in referral centers need to be prepared to appropriately provide neonatal resuscitation. A team of American Heart Association health care providers taught a two-day neonatal resuscitation curriculum designed for low-resource settings at a regional hospital in Liberia. The goal of this study was to evaluate if the curriculum improved knowledge and comfort in participation. The curriculum included simulations and was based on the Neonatal Resuscitation Protocol (NRP). Students learned newborn airway management, quality chest compression skills, and resuscitation interventions through lectures and manikin-based simulation sessions. Seventy-five participants were trained. There was a 63% increase in knowledge scores post training (p < 0.00001). Prior cardiopulmonary resuscitation (CPR) training, age, occupation, and pre-intervention test score did not have a significant effect on post-intervention knowledge test scores. The median provider comfort score improved from a 4 to 5 (p < 0.00001). Factors such as age, sex, prior NRP education, occupation, and post-intervention test scores did not have a significant effect on the post-intervention comfort level score. A modified NRP and manikin simulation-based curriculum may be an effective way of teaching health care providers in resource-limited settings. Training of providers in limited-resource settings could potentially help decrease neonatal mortality in Liberia. Modification of protocols is sometimes necessary and an important part of providing context-specific training. The results of this study have no direct relation to decreasing neonatal mortality until proven. A general resuscitation curriculum with modified NRP training may be effective, and further work should focus on the effect of such interventions on neonatal mortality rates in the region.
Influence of an MgTiTaON Inserted Layer on Magnetic Properties and Microstructure of FePtAgC Films
Authors: Tsai Jai-Lin , Dai Cheng , Chen Jyun-you , Hsu Ting-Wei , Weng Shi-Min , Huang Lin-Chen
Published: April 2019

The FePt film above 10 nm critical lattice relaxation thickness was prepared and the ultrathin MgTiTaON layer was interleaved in between FePt film and the multilayer stack is FePt(6 nm)/[MgTiTaON(1 nm)/FePt(4 nm)]2. Next, the FePt films were co-sputtered with (Ag, C) segregants during deposition and the layer stacks is FePt(6 nm)(Ag, C)(x vol %)/[MgTiTaON (1 nm)/FePt(4 nm)(Ag, C) (x vol %)]2 (x = 0, 10, 20, 30, 40). After high temperature deposition at 470 °C, the granular FePt(Ag, C, MgTiTaON) film illustrated perpendicular magnetization and the out-of-plane coercivity (Hc) was increased with (Ag, C) segregants and the highest Hc is 18.3 kOe when x = 40. From cross-section images, the FePt layer are more continuous with 0 and 10 vol % (Ag, C) segregants and changed to an island structure when the (Ag, C) segregants increase to 20–40 vol %. The FePt grains were grown in separated islands in 20, 30 vol % (Ag, C) and changed to dense columnar-like morphology in 40 vol %. The second nucleated grains which contribute the in-plane magnetization are found in FePt (Ag, C) (40 vol %) film. The FePt islands are reached by inserting the ultrathin MgTiTaON layer and the island heights of FePt(Ag, C) (30, 40 vol %) are around 31–38 nm and the aspect ratios are 0.6–0.8.
Time-Reversal Symmetry and Arrow of Time in Quantum Mechanics of Open Systems
Authors: Hatano Naomichi , Ordonez Gonzalo
Published: April 2019

It is one of the most important and long-standing issues of physics to derive the irreversibility out of a time-reversal symmetric equation of motion. The present paper considers the breaking of the time-reversal symmetry in open quantum systems and the emergence of an arrow of time. We claim that the time-reversal symmetric Schrödinger equation can have eigenstates that break the time-reversal symmetry if the system is open in the sense that it has at least a countably infinite number of states. Such eigenstates, namely the resonant and anti-resonant states, have complex eigenvalues. We show that, although these states are often called “unphysical”, they observe the probability conservation in a particular way. We also comment that the seemingly Hermitian Hamiltonian is non-Hermitian in the functional space of the resonant and anti-resonant states, and hence there is no contradiction in the fact that it has complex eigenvalues. We finally show how the existence of the states that break the time-reversal symmetry affects the quantum dynamics. The dynamics that starts from a time-reversal symmetric initial state is dominated by the resonant states for t > 0 ; this explains the phenomenon of the arrow of time, in which the decay excels the growth. The time-reversal symmetry holds in that the dynamic ending at a time-reversal symmetric final state is dominated by the anti-resonant states for t < 0 .
Understanding Cellular Respiration through Simulation Using Lego® as a Concrete Dynamic Model
Authors: Dam Michiel , Ottenhof Koen , Van Boxtel Carla , Janssen Fred
Published: April 2019

Out of all the complex systems in science education curricula, cellular respiration is considered to be one of the most complex and abstract processes. Students are known to have low interest and difficulties in conceptual understanding of cellular respiration which provides a challenge for teaching and learning. In this study, we took literature about modelling and teaching and learning cellular respiration as a starting point for the design of a concrete dynamic model in which students (n = 126) use Lego® to simulate the process of cellular respiration. Students used the simulation embedded in the context of finding the efficiency of a sediment battery as a future source of green energy and we tested the effects on conceptual learning and situational interest in an experimental study. Results on conceptual learning show that both experimental and control groups had comparable results in the test. The questions that students in the experimental group asked during enactment, however, gave notice of a focus on both isolated component parts as well as modes of organization at higher organizational levels which is linked to how biologists mechanistically understand complex systems. Both groups report a similar high measure to which the topic is meaningful in real life (situational interest value), whereas the enjoyment (situational interest feeling) was significantly increased in the experimental group. Furthermore, students report specific advantages (e.g., I now understand that one acid chemically changes into another and they do not just transfer atoms) and disadvantages (e.g., time issues).
Electronic Circuit with Controllable Negative Differential Resistance and its Applications
Authors: Ulansky Vladimir , Raza Ahmed , Oun Hamza
Published: April 2019

Electronic devices and circuits with negative differential resistance (NDR) are widely used in oscillators, memory devices, frequency multipliers, mixers, etc. Such devices and circuits usually have an N-, S-, or Λ-type current-voltage characteristics. In the known NDR devices and circuits, it is practically impossible to increase the negative resistance without changing the type or the dimensions of transistors. Moreover, some of them have three terminals assuming two power supplies. In this paper, a new NDR circuit that comprises a combination of a field effect transistor (FET) and a simple bipolar junction transistor (BJT) current mirror (CM) with multiple outputs is proposed. A distinctive feature of the proposed circuit is the ability to change the magnitude of the NDR by increasing the number of outputs in the CM. Mathematical expressions are derived to calculate the threshold currents and voltages of the N-type current-voltage characteristics for various types of FET. The calculated current and voltage thresholds are compared with the simulation results. The possible applications of the proposed NDR circuit for designing single-frequency oscillators and voltage-controlled oscillators (VCO) are considered. The designed NDR VCO has a very low level of phase noise and has one of the best values of a standard figure of merit (FOM) among recently published VCOs. The effectiveness of the proposed oscillators is confirmed by the simulation results and the implemented prototype.
A 3D Thermal Network Model for Monitoring Imbalanced Thermal Distribution of Press-Pack IGBT Modules in MMC-HVDC Applications
Authors: Chang Yao , Li Wuhua , Luo Haoze , He Xiangning , Iannuzzo Francesco , Blaabjerg Frede , Lin Weixing
Published: April 2019

In this paper, the impact of a double-sided press-pack insulated-gate-bipolar-transistor (PP IGBT) cooling structure on its thermal impedance distribution is studied and explored. A matrix thermal impedance network model is built by considering the multi-chip thermal coupling effect for the collector side of the PP IGBT. Moreover, a verification has been made by comparing the proposed matrix thermal network model and the conventional lumped RC network model provided by the manufacturer. It is concluded that the collector side has lower thermal resistance and dissipates about 88% of the heat generated by the IGBT chips inside the module. Then, a modular-multilevel-converter high-voltage-direct-current (MMC-HVDC)-based type test setup composed of the press-pack IGBT stacks is established and the junction temperature is calculated with the proposed thermal model and verified by temperature measurements.
Mathematical Modeling of Unsteady Gas Transmission System Operating Conditions under Insufficient Loading
Authors: Zapukhliak Vasyl , Poberezhny Lyubomyr , Maruschak Pavlo , Grudz Jr. Volodymyr , Stasiuk Roman , Brezinová Janette , Guzanová Anna
Published: April 2019

Under insufficient loading of a main gas transmission system, high-amplitude fluctuations of pressure may occur in it. A mathematical model is proposed to estimate the amplitude of pressure fluctuations in a gas pipeline along its length. It has been revealed that the shutdown of compressor stations along the gas pipeline route has a significant impact on the parameters of the unsteady transient operating conditions. The possibility of minimizing oscillation processes by disconnecting compressor stations is substantiated for the “Soyuz” main gas pipeline.
Case Studies of Rock Bursts in Tectonic Areas with Facies Change
Authors: Guo Weiyao , Gu Qingheng , Tan Yunliang , Hu Shanchao
Published: April 2019

Although tectonic areas with facies change (i.e., variation of coal seam thickness, coal seam dip angle, or coal quality) are one of the three major geological structures that induce rock bursts, case studies of rock bursts in these tectonic structures are rare. The main objective of this study is to illustrate this issue and provide case studies that may inspire future research. Based on several typical cases of rock bursts induced by tectonic areas with facies change, the conditions conducive for these bursts are introduced and investigated in detail. Subsequently, numerical simulation is performed, showing that stress concentration exists in regions with variable coal seam thicknesses or dip angle. When stoping or tunnelling approaches this region, the peak stress increases rapidly. Thus, the burst occurs via a mechanism involving the superposition of high in-situ stress from tectonic areas with facies change and abutment pressure from stoping or tunnelling, leading to high stress concentration. Strategies for mitigating rock bursts are also provided. Rock bursts induced by tectonic areas with facies change can be mitigated by avoiding regions of high tectonic stress concentration and reducing mining induced stress.
Fractal and Multifractal Analysis of Pore Size Distribution in Low Permeability Reservoirs Based on Mercury Intrusion Porosimetry
Authors: Su Penghui , Xia Zhaohui , Wang Ping , Ding Wei , Hu Yunpeng , Zhang Wenqi , Peng Yujie
Published: April 2019

To quantitatively evaluate the complexities and heterogeneities of pore structures in sandstone reservoirs, we apply single fractal theory and multifractal theory to explore the fractal characteristics of pore size distributions based on mercury intrusion porosimetry. The fractal parameters were calculated and the relationships between the petrophysical parameters (permeability and entry pressure) and the fractal parameters were investigated. The results show that the single fractal curves exhibit two-stage characteristics and the corresponding fractal dimensions D1 and D2 can characterize the complexity of pore structure in different sizes. Favorable linear relationships between log(ε) and log(μ,(ε)) indicate that the samples satisfy multifractal characteristics and ε is the sub-intervals with size ε = J × 2−k. The multifractal singularity curves used in this study exhibit a right shape, indicating that the heterogeneity of the reservoir is mainly affected by pore size distributions in sparse regions. Multifractal parameters, D(0), D(1), and Δf, are positively correlated with permeability and entry pressure, while D(0), D(1), and Δf are negatively correlated with permeability and entry pressure. The ratio of larger pores volumes to total pore volumes acts as a control on the fractal dimension over a specific pore size range, while the range of the pore size distribution has a definite impact on the multifractal parameters. Results indicate that fractal analysis and multifractal analysis are feasible methods for characterizing the heterogeneity of pore structures in a reservoir. However, the single fractal models ignore the influence of microfractures, which could result in abnormal values for calculated fractal dimension. Compared to single fractal analysis, multifractal theory can better quantitatively characterize the heterogeneity of pore structure and establish favorable relationships with reservoir physical property parameters.
A Flower Pollination Method Based Global Maximum Power Point Tracking Strategy for Point-Absorbing Type Wave Energy Converters
Authors: Zhao Aqiang , Wu Weimin , Sun Zuoyao , Zhu Lixun , Lu Kaiyuan , Chung Henry , Blaabjerg Frede
Published: April 2019

To maximize the generated output power under random waves, the control strategy of maximum power point tracking (MPPT) is incorporated in point-absorbing type wave energy converter (WEC) system. However, due to the influence of mutative wave conditions, the curve of the maximum average power appears in multiple peaks, adding complexity to the tracking process. This paper proposes a new MPPT control technique for a wave power generation system, by using the flower pollination algorithm (FPA) instead of the conventional hill-climbing method. Compared with the hill-climbing method, this method has advantages of achieving a smaller peak-to-average ratio and capturing the more average power under the same sea state. The verification has been carried out through the simulations and the experimental results on a lab test bench.
Modeling the Relationship between Crude Oil and Agricultural Commodity Prices
Authors: Vo Duc Hong , Vu Tan Ngoc , Vo Anh The , McAleer Michael
Published: April 2019

The food-energy nexus has attracted great attention from policymakers, practitioners, and academia since the food price crisis during the 2007–2008 Global Financial Crisis (GFC), and new policies that aim to increase ethanol production. This paper incorporates aggregate demand and alternative oil shocks to investigate the causal relationship between agricultural products and oil markets. For the period January 2000–July 2018, monthly spot prices of 15 commodities are examined, including Brent crude oil, biofuel-related agricultural commodities, and other agricultural commodities. The sample is divided into three sub-periods, namely: (i) January 2000–July 2006, (ii) August 2006–April 2013, and (iii) May 2013–July 2018. The structural vector autoregressive (SVAR) model, impulse response functions, and variance decomposition technique are used to examine how the shocks to agricultural markets contribute to the variance of crude oil prices. The empirical findings from the paper indicate that not every oil shock contributes the same to agricultural price fluctuations, and similarly for the effects of aggregate demand shocks on the agricultural market. These results show that the crude oil market plays a major role in explaining fluctuations in the prices and associated volatility of agricultural commodities.
Analysis of Cable Screen Currents for Diagnostics Purposes
Authors: Lowczowski Krzysztof , Nadolny Zbigniew , Olejnik Bartosz
Published: April 2019

This paper presents current flow in cable screens and a cable screen earthing system. Moreover, the paper presents the methodology for the detection of problems in cable screens, such as open phase in a cable screen and high contact resistance of a cable screen in a cable joint. The is based on cable screen current measurements and allows for localization of the erroneous connection–high contact resistance or open phase. The phenomenon is simulated in PowerFactory Software. Moreover, exemplary cable screen current measurements are presented.
Using Deep Dive Methodology to Investigate an Increased Incidence of Hospital-Acquired Avoidable Category 2 and 3 Pressure Ulcers
Authors: Horton-Jones Martyne , Marsh Emma , Fumarola Sian , Wright-White Helen , McSherry Wilfred , Rowson Trish
Published: April 2019

Background: Between October 2017 and March 2018, the Trust experienced significant winter pressures and an increase in category 2 and 3 hospital-acquired avoidable pressure ulcers. This review aimed to investigate the causal factors of this increase. Methods: A ‘Deep Dive’ review of 37 cases was undertaken in three stages: (i) assurance; ensure the increase was not due to insufficient equipment; (ii) collation of relevant data, including age, length of time in A&E, bed surface, number of internal moves; (iii) analysis identifying factors that might account for the observed increase. Findings: Age combined with prolonged length of time in A&E, being nursed on a trolley followed by three or four internal moves were observed in patients who developed pressure ulcers. Patient age was observed as a key factor, with those over 80 years experiencing pressure ulcers more frequently. Conclusion: The small size of this data suggests a need for the greater awareness of frailty issues in older people, timely assessment and intervention to prevent a chain of detrimental factors might be key to reduce and prevent hospital-acquired avoidable pressure ulcers. Recommendations for immediate action, education and future research have been made to the Trust Quality and Safety Committee.
Use of Machine Learning in the Analysis of Indoor ELF MF Exposure in Children
Authors: Tognola Gabriella , Bonato Marta , Chiaramello Emma , Fiocchi Serena , Magne Isabelle , Souques Martine , Parazzini Marta , Ravazzani Paolo
Published: April 2019

Characterization of children exposure to extremely low frequency (ELF) magnetic fields is an important issue because of the possible correlation of leukemia onset with ELF exposure. Cluster analysis—a Machine Learning approach—was applied on personal exposure measurements from 977 children in France to characterize real-life ELF exposure scenarios. Electric networks near the child’s home or school were considered as environmental factors characterizing the exposure scenarios. The following clusters were identified: children with the highest exposure living 120–200 m from 225 kV/400 kV overhead lines; children with mid-to-high exposure living 70–100 m from 63 kV/150 kV overhead lines; children with mid-to-low exposure living 40 m from 400 V/20 kV substations and underground networks; children with the lowest exposure and the lowest number of electric networks in the vicinity. 63–225 kV underground networks within 20 m and 400 V/20 kV overhead lines within 40 m played a marginal role in differentiating exposure clusters. Cluster analysis is a viable approach to discovering variables best characterizing the exposure scenarios and thus it might be potentially useful to better tailor epidemiological studies. The present study did not assess the impact of indoor sources of exposure, which should be addressed in a further study.
WHO Thematic Platform for Health Emergency and Disaster Risk Management Research Network (TPRN): Report of the Kobe Expert Meeting
Authors: Kayano Ryoma , Chan Emily YY , Murray Virginia , Abrahams Jonathan , Barber Sarah Louise
Published: April 2019

The WHO Thematic Platform for Health Emergency and Disaster Risk Management Research Network (TPRN) was established in 2016 in response to the Sendai Framework for Disaster Risk Reduction 2015–2030. The TPRN facilitates global collaborative action for improving the scientific evidence base in health emergency and disaster risk management (Health EDRM). In 2018, the WHO convened a meeting to identify key research questions, bringing together leading experts from WHO, TPRN, World Association for Disaster and Emergency Medicine (WADEM), and the Japan International Cooperation Agency, and delegates to the Asia Pacific Conference on Disaster Medicine (APCDM). The meeting identified research questions in five major areas for Health EDRM: health data management, psychosocial management, community risk management, health workforce development, and research methods and ethics. Funding these key research questions is essential to accelerate evidence-based actions during emergencies and disasters.
The Role of Religion in Buffering the Impact of Stressful Life Events on Depressive Symptoms in Patients with Depressive Episodes or Adjustment Disorder
Authors: Lorenz Louisa , Doherty Anne , Casey Patricia
Published: April 2019

Most studies into the role of religiousness in relation to depression severity have mainly found an inverse relationship between greater religiousness and lower levels of depressive symptoms. There is reason to assume that religiousness has a buffering effect on the relationship between stressful life events and depressive symptoms. The aim of this study was to investigate the role of religiousness in moderating the impact of stressors on depressive symptoms. n = 348 patients with either a depressive episode or adjustment disorder were assessed at referral to the liaison psychiatry services in three Dublin hospitals and n = 132 patients were followed up six months later. We assessed depressive symptoms, life events, social support, and religiosity, and used hierarchical and multiple linear regression for data analysis. The interaction of organised religious activity and the amount of life events was significant (β = −0.19, p = 0.001) in the cross-sectional prediction of depressive symptoms while non-organised religious activity (β = −0.23, p = 0.001) and intrinsic religiousness (β = −0.15, p = 0.033) interacted significantly with life events in the longitudinal analysis. This study demonstrated that various dimensions of religiousness buffered the impact of life events on outcome.
Hydrophobic Amines and Their Guanidine Analogues Modulate Activation and Desensitization of ASIC3
Authors: Shteinikov Vasilii Y , Potapieva Natalia N , Gmiro Valery E , Tikhonov Denis B
Published: April 2019

Acid-sensing ion channel 3 (ASIC3) is an important member of the acid-sensing ion channels family, which is widely expressed in the peripheral nervous system and contributes to pain sensation. ASICs are targeted by various drugs and toxins. However, mechanisms and structural determinants of ligands’ action on ASIC3 are not completely understood. In the present work we studied ASIC3 modulation by a series of “hydrophobic monoamines” and their guanidine analogs, which were previously characterized to affect other ASIC channels via multiple mechanisms. Electrophysiological analysis of action via whole-cell patch clamp method was performed using rat ASIC3 expressed in Chinese hamster ovary (CHO) cells. We found that the compounds studied inhibited ASIC3 activation by inducing acidic shift of proton sensitivity and slowed channel desensitization, which was accompanied by a decrease of the equilibrium desensitization level. The total effect of the drugs on the sustained ASIC3-mediated currents was the sum of these opposite effects. It is demonstrated that drugs’ action on activation and desensitization differed in their structural requirements, kinetics of action, and concentration and state dependencies. Taken together, these findings suggest that effects on activation and desensitization are independent and are likely mediated by drugs binding to distinct sites in ASIC3.
Development and Palatability Assessment of Norvir® (Ritonavir) 100 mg Powder for Pediatric Population
Authors: Morris John B. , Tisi David A. , Tan David Cheng Thiam , Worthington Jeffrey H.
Published: April 2019

Norvir® (ritonavir) is a Biopharmaceutical Classification System Class IV compound with poor solubility in water (~5 µg/mL) and limited oral bioavailability. Early stage development efforts were focused on an oral solution (OS) which provided reasonable bioavailability but exhibited taste-masking challenges and required the use of solvents with potential pediatric toxicity. Norvir® oral powder, 100 mg (NOP) was developed to replace OS. The objective of this study is to provide an overview of the development of NOP and palatability assessment strategy. Palatability of NOP was assessed using the flavor profile method: (1) As an aqueous suspension dose/response and (2) evaluation with foods. The dose/response sensory analysis indicated that NOP has strong intensity bitterness and burnt aromatics (3 on the 0–3 flavor profile scale) at the clinical dose (100 mg/10 mL) and the recognition threshold was determined to be 0.3 mg/10 mL. To improve palatability, 100 mg/10 mL NOP aqueous suspension was evaluated with foods. Consuming foods high in fat and/or sugar content after NOP administration successfully reduced bitterness to a 1.5 intensity. In summary, NOP provides dose flexibility, enhanced stability, eliminated solvents, and maintains consistent bioavailability, with reduced bitterness and improved palatability via administration with common food products.
Comparison of Fatty Acid Profiles in a Group of Female Patients with Chronic Kidney Diseases (CKD) and Metabolic Syndrome (MetS)–Similar Trends of Changes, Different Pathophysiology
Authors: Szczuko Małgorzata , Kaczkan Małgorzata , Drozd Arleta , Maciejewska Dominika , Palma Joanna , Owczarzak Anna , Marczuk Natalia , Rutkowski Przemysław , Małgorzewicz Sylwia
Published: April 2019

Fatty acid (FA) profiles in the plasma of patients with metabolic syndrome and chronic kidney disease (CKD) seem to be identical despite their different etiology (dietary mistakes vs. cachexia). The aim of this study was to compare both profiles and to highlight the differences that could influence the improvement of the treatment of patients in both groups. The study involved 73 women, including 24 patients with chronic kidney disease treated with haemodialysis, 19 patients with metabolic syndrome (MetS), and 30 healthy women in the control group. A total of 35 fatty acids and derivatives were identified and quantified by gas chromatography. Intensified elongation processes from acid C10:0 to C16:0 were noted in both groups (more intense in MetS), as well as an increased synthesis of arachidonic acid (C20:4n6), which was more intense in CKD. Significant correlations of oleic acid (C18:1n9), gamma linoleic acid (C18:3n6), and docosatetraenoate acid (C22:4n6) with parameters of CKD patients were observed. In the MetS group, auxiliary metabolic pathways of oleic acid were activated, which simultaneously inhibited the synthesis of eicosapentanoic acid (EPA) and docosahexaenoic acid (DHA) from alpha lipoic acid (ALA). On the other hand, in the group of female patients with CKD, the synthesis of EPA and DHA was intensified. Activation of the synthesis of oleic acid (C18: 1n9 ct) and trans-vaccinic acid (C18:1) is a protective mechanism in kidney diseases and especially in MetS due to the increased concentration of saturated fatty acid (SFA) in plasma. The cause of the increased amount of all FAs in plasma in the CKD group, especially in the case of palmitic (C16:0) and derivatives stearic (C18:0) acids, may be the decomposition of adipose tissue and the progressing devastation of the organism, whereas, in the MetS group, dietary intake seems to be the main reason for the increase in SFA. Moreover, in MetS, auxiliary metabolic pathways are activated for oleic acid, which cause the simultaneous inhibition of EPA and DHA synthesis from ALA, whereas, in the CKD group, we observe an increased synthesis of EPA and DHA. The higher increase of nervonic acid (C24:1) in CKD suggests a higher degree of demyelination and loss of axons.
The Role of Fibrosis and Liver-Associated Fibroblasts in the Pathogenesis of Hepatocellular Carcinoma
Authors: Baglieri Jacopo , Brenner David A. , Kisseleva Tatiana
Published: April 2019

Hepatocellular carcinoma (HCC) is one of the most aggressive types of cancer and lacks effective therapeutic approaches. Most HCC develops in the setting of chronic liver injury, hepatic inflammation, and fibrosis. Hepatic stellate cells (HSCs) and cancer-associated fibroblasts (CAFs) are key players in liver fibrogenesis and hepatocarcinogenesis, respectively. CAFs, which probably derive from HSCs, activate into extracellular matrix (ECM)-producing myofibroblasts and crosstalk with cancer cells to affect tumor growth and invasion. In this review, we describe the different components which form the HCC premalignant microenvironment (PME) and the tumor microenvironment (TME), focusing on the liver fibrosis process and the biology of CAFs. We will describe the CAF-dependent mechanisms which have been suggested to promote hepatocarcinogenesis, such as the alteration of ECM, CAF-dependent production of cytokines and angiogenic factors, CAF-dependent reduction of immuno-surveillance, and CAF-dependent promotion of epithelial-mesenchymal transition (EMT). New knowledge of the fibrosis process and the role of CAFs in HCC may pave the way for new therapeutic strategies for liver cancer.
Identification and Expression Profiling of the Regulator of Chromosome Condensation 1 (RCC1) Gene Family in Gossypium Hirsutum L. under Abiotic Stress and Hormone Treatments
Authors: Liu Xiao , Wu Xingchen , Sun Chendong , Rong Junkang
Published: April 2019

The regulator of chromosome condensation 1 (RCC1) is the nucleotide exchange factor for a GTPase called the Ras-related nuclear protein, and it is important for nucleo-plasmic transport, mitosis, nuclear membrane assembly, and control of chromatin agglutination during the S phase of mitosis in animals. In plants, RCC1 molecules act mainly as regulating factors for a series of downstream genes during biological processes such as the ultraviolet-B radiation (UV-B) response and cold tolerance. In this study, 56 genes were identified in upland cotton by searching the associated reference genomes. The genes were found to be unevenly distributed on 26 chromosomes, except A06, A12, D03, and D12. Phylogenetic analysis by maximum-likelihood revealed that the genes were divided into five subgroups. The RCC1 genes within the same group shared similar exon/intron patterns and conserved motifs in their encoded proteins. Most genes of the RCC1 family are expressed differently under various hormone treatments and are negatively controlled by salt stress. Gh_A05G3028 and Gh_D10G2310, which encode two proteins located in the nucleus, were strongly induced under salt treatment, while mutants of their homoeologous gene (UVR8) in Arabidopsis and VIGS (virus induced gene silencing) lines of the two genes above in G. hirsutum exhibited a salt-sensitive phenotype indicating their potential role in salt resistance in cotton. These results provide valuable reference data for further study of RCC1 genes in cotton.
HvPAA1 Encodes a P-Type ATPase, a Novel Gene for Cadmium Accumulation and Tolerance in Barley (Hordeum vulgare L.)
Authors: Wang Xin-Ke , Gong Xue , Cao Fangbin , Wang Yizhou , Zhang Guoping , Wu Feibo
Published: April 2019

The identification of gene(s) that are involved in Cd accumulation/tolerance is vital in developing crop cultivars with low Cd accumulation. We developed a doubled haploid (DH) population that was derived from a cross of Suyinmai 2 (Cd-sensitive) × Weisuobuzhi (Cd-tolerant) to conduct quantitative trait loci (QTL) mapping studies. We assessed chlorophyll content, traits that are associated with development, metal concentration, and antioxidative enzyme activity in DH population lines and parents under control and Cd stress conditions. A single QTL, designated as qShCd7H, was identified on chromosome 7H that was linked to shoot Cd concentration; qShCd7H explained 17% of the phenotypic variation. Comparative genomics, map-based cloning, and gene silencing were used in isolation, cloning, and functional characterization of the candidate gene. A novel gene HvPAA1, being related to shoot Cd concentration, was identified from qShCd7H. Sequence comparison indicated that HvPAA1 carried seven domains with an N-glycosylation motif. HvPAA1 is predominantly expressed in shoots. Subcellular localization verified that HvPAA1 is located in plasma membrane. The silencing of HvPAA1 resulted in growth inhibition, greater Cd accumulation, and a significant decrease in Cd tolerance. We conclude HvPAA1 is a novel plasma membrane-localized ATPase that contributes to Cd tolerance and accumulation in barley. The results provide us with new insights that may aid in the screening and development of Cd-tolerant and low-Cd-accumulation crops.
The Role of Melatonin in Salt Stress Responses
Authors: Li Junpeng , Liu Jing , Zhu Tingting , Zhao Chen , Li Lingyu , Chen Min
Published: April 2019

Melatonin, an indoleamine widely found in animals and plants, is considered as a candidate phytohormone that affects responses to a variety of biotic and abiotic stresses. In plants, melatonin has a similar action to that of the auxin indole-3-acetic acid (IAA), and IAA and melatonin have the same biosynthetic precursor, tryptophan. Salt stress results in the rapid accumulation of melatonin in plants. Melatonin enhances plant resistance to salt stress in two ways: one is via direct pathways, such as the direct clearance of reactive oxygen species; the other is via an indirect pathway by enhancing antioxidant enzyme activity, photosynthetic efficiency, and metabolite content, and by regulating transcription factors associated with stress. In addition, melatonin can affect the performance of plants by affecting the expression of genes. Interestingly, other precursors and metabolite molecules associated with melatonin can also increase the tolerance of plants to salt stress. This paper explores the mechanisms by which melatonin alleviates salt stress by its actions on antioxidants, photosynthesis, ion regulation, and stress signaling.
Global MicroRNA Profiling Uncovers miR-206 as a Negative Regulator of Hematopoietic Commitment in Human Pluripotent Stem Cells
Authors: Flamant Stéphane , Chomel Jean-Claude , Desterke Christophe , Féraud Olivier , Gobbo Emilie , Mitjavila-Garcia Maria-Teresa , Foudi Adlen , Griscelli Frank , Turhan Ali G. , Bennaceur-Griscelli Annelise
Published: April 2019

Although human pluripotent stem cells (hPSCs) can theoretically differentiate into any cell type, their ability to produce hematopoietic cells is highly variable from one cell line to another. The underlying mechanisms of this heterogeneity are not clearly understood. Here, using a whole miRNome analysis approach in hPSCs, we discovered that their hematopoietic competency was associated with the expression of several miRNAs and conversely correlated to that of miR-206 specifically. Lentiviral-based miR-206 ectopic expression in H1 hematopoietic competent embryonic stem (ES) cells markedly impaired their differentiation toward the blood lineage. Integrative bioinformatics identified a potential miR-206 target gene network which included hematopoietic master regulators RUNX1 and TAL1. This work sheds light on the critical role of miR-206 in the generation of blood cells off hPSCs. Our results pave the way for future genetic manipulation of hPSCs aimed at increasing their blood regenerative potential and designing better protocols for the generation of bona fide hPSC-derived hematopoietic stem cells.
pH-Triggered Interfacial Interaction of Kaolinite/Chitosan Nanocomposites with Anionic Azo Dye
Authors: Dey Shaikat Chandra , Moztahida Mokrema , Sarker Mithun , Ashaduzzaman Md. , Shamsuddin Sayed Md.
Published: April 2019

Advanced engineering of naturally occurring materials opens new doors in nanoscience and nanotechnology for the separation and/or removal of environmental hazards. Here, a series of nanocomposites containing kaolinite and chitosan varying in the range of 20 to 80% (w/w) kaolinite were used for the adsorptive removal of a reactive textile dye, Remazol Red, from an aqueous solution. Batch experiments were carried out to investigate the effects of pH, contact time, and initial dye concentration on the adsorption capacity. Nanocomposites containing 80% kaolinite (w/w) and 20% chitosan (w/w), i.e., NK80C20, showed an equilibrium adsorption capacity of 371.8 mg/g at pH 2.5, which was 5.2 times higher than that of commercial activated charcoal. Moreover, NK80C20 was regenerated instantly up to 99.9% at pH 10. Therefore, NK80C20 can be effectively utilized as a potential adsorbent for the separation of Remazol Red and homologous azo dyes from industrial effluents. We expect that the findings from this study will play a vital role in environmental research leading to advanced applications in water purification.
Spillover Effects of US QE and QE Tapering on African and Middle Eastern Stock Indices
Authors: Papadamou Stephanos , Kyriazis Nikolaos , Tzeremes Panayiotis
Published: April 2019

In this paper, we study spillover effects on the stock markets of six African and nine Middle Eastern emerging economies before, during, and after the implementation of unconventional monetary policies by the United States Federal Reserve (US Fed). Weekly data covering the pre-quantitative easing (pre-QE) period, the three phases of QE, and the QE-tapering period were adopted. The methodologies employed here for detecting dual causality were as follows: classical, dynamic, and time-varying Granger causality tests. The results indicate that the Fed’s non-conventional actions weakened the Fed’s monetary policies’ impact on the stock indices of these emerging countries. Interestingly, there is evidence that two African and three Middle Eastern countries—all different according to each of the specifications used in the methodology—were affected by the tapering of unconventional US monetary practices.
Applications and Advances of Magnetoelastic Sensors in Biomedical Engineering: A Review
Authors: Ren Limin , Yu Kun , Tan Yisong
Published: April 2019

We present a comprehensive investigation into magnetoelastic sensors (MES) technology applied to biomedical engineering. This includes the working principles, detection methods, and application fields of MES technology. MES are made of amorphous metallic glass ribbons and are wireless and passive, meaning that it is convenient to monitor or measure the parameters related to biomedical engineering. MES are based on the inverse magnetoelastic (Villari) effect. When MES are subjected to mechanical stress, their magnetic susceptibility will change accordingly. And the susceptibility of MES is directly related to their magnetic permeability. The varying permeability can positively reflect the applied stress. The various detection methods that have been developed for different field applications include measurement of force, stress, and strain, monitoring of various chemical indexes, and consideration of different biomedical parameters such as the degradation rate and force conditions of artificial bone, as well as various physiological indexes including ammonia level, glucose concentration, bacteria growth, and blood coagulation.
Novel Ultralight-Weight Complex Concentrated Alloys with High Strength
Authors: Jia Yuefei , Jia Yandong , Wu Shiwei , Ma Xindi , Wang Gang
Published: April 2019

To explore a novel high strength and low modulus ultralight-weight complex concentrated alloys (ULW-CCAs), a series of light alloys are designed and explored based on some low-density and low modulus elements, such as Al, Li, Mg, Ca, Si, and Y. An Al19.9Li30Mg35Si10Ca5Y0.1 (at %) CCA with a high specific strength of 327 KPa·m−3 is successfully developed. After adjusting the composition, the Al15Li35Mg48Ca1Si1 CCA with the good compressive plasticity is successfully developed. The Al15Li38Mg45Ca0.5Si1.5 and Al15Li39Mg45Ca0.5Si0.5 CCAs exhibit good plasticity of >45%, and >60%, respectively. These ULW-CCAs show the high specific strength, good ductility, and low Young’s modulus, as compared with the previously reported CCAs.
Advanced Laguerre Tessellation for the Reconstruction of Ceramic Foams and Prediction of Transport Properties
Authors: Stiapis Christos S. , Skouras Eugene D. , Burganos Vasilis N.
Published: April 2019

Ceramic foams are promising, highly porous materials, with a wide range of specific surface area and low fluid flow resistance, which are well-suited for filtering applications. They are comprised mainly of macrovoids that are interconnected with struts. A branch-shaped reconstruction algorithm is introduced in the present work to reconstruct various ceramic foams from electron microscopy images using the Laguerre tessellation method. Subsequently, the reconstructed samples are used for the numerical calculation of pore structure and transport properties, including specific surface area, tortuosity, effective diffusivity, and flow permeability. Following comparison with experimental data, this reconstruction method is shown to be more reliable than typical analytical expressions that are suggested in the literature for the aforementioned structural and transport properties. Extracting the equivalent pore radius of the reconstructed domains offers improved accuracy of the analytical expressions for the permeability estimation.
Enhancing Thermal Oxidation Stability of Silver Nanowire Transparent Electrodes by Using a Cesium Carbonate-Incorporated Overcoating Layer
Authors: Jeong Yong-Chan , Nam Jiyoon , Kim Jongbok , Kim Chang Su , Jo Sungjin
Published: April 2019

Despite their excellent electrical and optical properties, Ag nanowires (NWs) suffer from oxidation when exposed to air for several days. In this study, we synthesized a Cs carbonate-incorporated overcoating layer by spin-coating and ultraviolet curing to prevent the thermal oxidation of Ag NWs. Cs incorporation increased the decomposition temperature of the overcoating layer, thus enhancing its thermal resistance. The effects of the Cs carbonate-incorporated overcoating layer on the optoelectrical properties and stability of Ag NWs were investigated in detail. The Ag NW electrode reinforced with the Cs carbonate-incorporated overcoating layer exhibited excellent thermal oxidation stability after exposure to air for 55 days at 85 °C and a relative humidity of 85%. The novel overcoating layer synthesized in this study is a promising passivation layer for Ag NWs against thermal oxidation under ambient conditions. This overcoating layer can be applied in large-area optoelectronic devices based on Ag NW electrodes.