bluuebunny/crossref_metadata_2025 huggingface dataset:
Malignant Transformation and Metastatic Spread of Dumbbell-Shaped Meningeal Melanocytoma of the Cervical Spine: A Case Report and Literature Review
Authors: Deng Shuang-lin , Wang Yu-bo , Wang Dan-hua , Zhan Shuang , Jing Yi , Guan Yi
Published: March 2022

Background

Meningeal melanocytoma is a rare disease that originates from leptomeningeal melanocytes in the central nervous system. Meningeal melanocytoma is generally considered benign, and has a good prognosis following complete surgical resection. Reports of the malignant transformation and spread of these tumors are scarce.

Case Presentation

A 19 year old female presented with headache, progressive limb weakness, and dyspnea. Magnetic resonance imaging showed a dumbbell-shaped lesion at C1–C2 that was hyperintense on T1 weighted images and showed strong contrast enhancement. Total resection was achieved using a posterior midline approach. Post-operative pathology showed meningeal melanocytoma. The tumor recurred 9 months later with intracranial spread. Resection of the lesion revealed malignant transformation to meningeal melanoma.

Conclusion

Meningeal melanocytoma harbors malignant potential even with total resection. Radiotherapy could be considered to prevent disease recurrence and progression.
Impact of COVID-19 on Global Kidney Transplantation Service Delivery: Interim Report
Authors: Papalois Vassilios , Kotton Camille N. , Budde Klemens , Torre-Cisneros Julian , Croce Davide , Halleck Fabian , Roze Stéphane , Grossi Paolo
Published: March 2022

This article gives a personal, historical, account of the impact of the COVID-19 pandemic on transplantation services. The content is based on discussions held at two webinars in November 2020, at which kidney transplantation experts from prestigious institutions in Europe and the United States reflected on how the pandemic affected working practices. The group discussed adaptations to clinical care (i.e., ceasing, maintaining and re-starting kidney transplantations, and cytomegalovirus infection management) across the early course of the pandemic. Discussants were re-contacted in October 2021 and asked to comment on how transplantation services had evolved, given the widespread access to COVID-19 testing and the roll-out of vaccination and booster programs. By October 2021, near-normal life and service delivery was resuming, despite substantial ongoing cases of COVID-19 infection. However, transplant recipients remained at heightened risk of COVID-19 infection despite vaccination, given their limited response to mRNA vaccines and booster dosing: further risk-reduction strategies required exploration. This article provides a contemporaneous account of these different phases of the pandemic from the transplant clinician’s perspective, and provides constructive suggestions for clinical practice and research.
Transformation of Chromium Speciation during High Hexavalent Chromium-Contaminated Soil Remediation by CPS and Biostimulation
Authors: Wu Qing , Wan Ruihan , Li Qiannan , Mo Wenjun , Liu Jieqiong , Zhao Caiyun , Peng Sen
Published: March 2022

To address the secondary pollution problem of chemical reduction and the defects of a long bioremediation period for the soil around a chromium (Cr) salt plant in China, calcium polysulfide (CPS) combined with biostimulation (adding nutrient solution with glucose and urea) was used to reduce and stabilize hexavalent chromium [Cr(VI)] in the soil. The results showed that the remediation effect of adding CPS and nutrient solution was better than that of CPS alone for Cr(VI)-contaminated soil. An amount of 2.15% CPS with the Cr(VI) concentration reduced to approximately 330.0 mg/kg was selected as the optimal reducing agent dosage. Following the addition of 11 g/kg glucose and 13 g/kg urea, the degradation rate of Cr(VI) reached over 92% after 3 days, and the water-soluble fraction decreased by 95.6% after 40 days. The stability of the Cr was significantly enhanced. The correlation and regression analyses of Cr speciation indicated that the water-soluble fraction had significant negative correlations with the iron-manganese (Fe-Mn) oxide-bound fraction and the organic matter-bound fraction. There were significant negative correlations between the exchangeable fraction and the carbonate-bound fraction as well as extremely significant positive correlations between the Fe-Mn oxide-bound fraction and the organic matter-bound fraction. It was speculated that both the Fe-Mn oxide-bound fraction and the organic matter-bound fraction were transformed from the water-soluble fraction, and the exchangeable fraction and the carbonate-bound fraction were mutually transformed. This study enables us to acquire more knowledge about the speciation transformation of Cr in soil and provides an efficient, low-cost, and low-risk technology for the remediation of high Cr(VI)-contaminated soil.
Response in Physiological Traits and Antioxidant Capacity of Two Cotton Cultivars under Water Limitations
Authors: Eid Mohamed A. M. , El-hady Mohamed A. Abd , Abdelkader Mohamed A. , Abd-Elkrem Yasser M. , El-Gabry Yasser A. , El-temsah Mohamed E. , El-Areed Sherif R. M. , Rady Mostafa M. , Alamer Khalid H. , Alqubaie Ahmad I. , Ali Esmat F.
Published: March 2022

Deficit irrigation water (DW) is one of the main stress factors that negatively affect cotton cultivation. Hence, the identification of cotton cultivars tolerant to DW and sandy soil conditions is particularly needed. Understanding the response of cultivars to DW is essential for estimating water needs. Besides, by understanding the physiological and antioxidant status, reflecting distinct growth, yield, and fiber quality traits under DW, the cultivar tolerant to DW can be identified in the early stage of plant growth. Therefore, two cotton cultivars (Giza 86 and Giza 92, selected for their suitability to the climatic conditions of the study area) were evaluated in this study under two DW regimes (80% or 60% of crop evapotranspiration; ETc) vs. complete irrigation water (CW; 100% of ETc as a control). These regimes amounted to 1228 or 922 vs. 1536 mm season−1, respectively, for field trials conducted during the 2019 and 2020 summer seasons. DW (80% or 60% of ETc) significantly decreased relative water content, membrane stability index, chlorophyll content, plant height, yield components, and fiber quality traits. Otherwise, phenolic compounds, proline contents, as well as antioxidant enzyme activities increased in concomitance with an increase in electrolyte leakage and malondialdehyde content. The harmful effects of the higher DW (60% of ETc) were more pronounced in both cultivars. However, compared to Giza 86, Giza 92 showed higher performance under both CW and DW regimes, accounting for higher values for all studied traits in the blooming stage. The correlation coefficient showed that most of the physiological traits and antioxidants under study were effective criteria in identifying a high-yielding cultivar under DW in the cotton blooming stage and therefore can be used to select the cotton cultivar more suitable for the conditions of the study area. Biplot analysis was used to study the relationship between DW and all evaluated traits, as it was found that the most prominent traits were elongation (%) with Giza 92 + 100% ETc, yellowness degree with Giza 86 + 100% ETc, and SOD with Giza 92 + 60% ETc.
Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.)
Authors: Kim JangChol , An Xin , Yang Ke , Miao Si , Qin Yushi , Hu Yinxia , Du Bo , Zhu Lili , He Guangcun , Chen Rongzhi
Published: March 2022

Brown planthopper (BPH) has become the most devastating insect pests of rice and a serious threat to rice production. To combat newly occurring virulent BPH populations, it is still urgent to explore more new broad-spectrum BPH resistance genes and integrate them into rice cultivars. In the present study, we explored the genetic basis of BPH resistance in IRGC 8678. We identified and mapped a new resistance gene Bph43 to a region of ~380 kb on chromosome 11. Genes encoding nucleotide-binding domain leucine-rich repeat-containing (NBS-LRR)-type disease resistance proteins or Leucine Rich Repeat family proteins annotated in this region were predicted as the possible candidates for Bph43. Meanwhile, we developed near isogenic lines of Bph43 (NIL-Bph43-9311) in an elite restorer line 9311 background using marker-assisted selection (MAS). The further characterization of NIL-Bph43-9311 demonstrated that Bph43 confers strong antibiosis and antixenosis effects on BPH. Comparative transcriptome analysis revealed that genes related to the defense response and resistance gene-dependent signaling pathway were significantly and uniquely enriched in BPH-infested NIL-Bph43-9311. Our work demonstrated that Bph43 can be deployed as a valuable donor in BPH resistance breeding programs.
Association between Temperature and Reproductive Fitness of Diaphorina citri Infected with Candidatus Liberibacter Asiaticus
Authors: Hussain Mubasher , Bano Summyya , Qasim Muhammad , Gao Jing , Arthurs Steven P. , Wang Liande , Qiu Dongliang , Mao Runqian
Published: March 2022

Diaphorina citri is a serious insect pest of citrus and an insect vector of Candidatus Liberibacter asiaticus (CLas) that causes Huanglongbing disease (citrus greening). In this study, we investigated the effect of the CLas pathogen on the life history parameters of D. citri at different temperature regimes. Our results demonstrated that the survival rate of first to fifth instar CLas-positive and CLas-negative D. citri fluctuate with the change in temperature over the range of 16–35 °C. Meanwhile, the mean developmental time (52.5 d) (d = day(s)) and adult longevity (5.2 d) of the CLas-positive psyllids was longer as compared to CLas-negative psyllids mean developmental time (32.81 d) and adult longevity (3.50 d) at the low- and high-temperature regimes (16 and 35 °C). However, at high temperature regimes, the significant effect of CLas-bacteria on D. citri fecundity was higher than the corresponding non-significant effect on their survivorship when compared to non-vectored psyllids. These results indicate a long-term, stable evolutionary relationship among vector-pathogen and climate change.
Effects of Dietary Chromium Picolinate on Gut Microbiota, Gastrointestinal Peptides, Glucose Homeostasis, and Performance of Heat-Stressed Broilers
Authors: Wang Guangju , Li Xiumei , Zhou Ying , Feng Jinghai , Zhang Minhong
Published: March 2022

The current research was devoted to evaluating the effects on gut microbiota, gastrointestinal peptides, and glucose homeostasis of chromium picolinate applied to heat-stressed broilers. In a 14 d experiment, 220 28-day-old AA broilers were randomly assigned into one thermal-neutral and three high-temperature groups dietary-supplemented with 0, 0.4, or 0.8 mg/kg of chromium as chromium picolinate. The temperature for the thermal-neutral group was set at 21 °C, while that for the other three groups (high temperature) was set at 31 °C. The results showed that the average daily gain and average daily feed intake of the 0.4 mg/kg chromium-supplemented group significantly increased compared with the high-temperature groups (p < 0.05). The content of cholecystokinin in the 0.4 mg/kg group significantly decreased, and the gastric inhibitory polypeptide level was significantly elevated in jejunum (p < 0.05). The cecal microbiota of heat-stressed broilers was substantially different from that of the thermal-neutral group. After diet-supplemented chromium, compared to the high-temperature groups, the 0.4 mg/kg chromium supplemented group was characterized by a reduction of Actinobacteriota and Proteobacteria at the phylum level. The Bacilli were elevated, while proportions of Coriobacteria and Gammaproteobacteria were reduced significantly at the class level. The proportions of Lactobacillaceae, Christensenellaceae, and Erysipelotrichaceae were elevated significantly, while that of Clostridiaceae was reduced significantly at the family level. The proportion of Turicibacter was elevated significantly and the proportions of Olsenella and Ruminococcus were reduced significantly at the genus level (p < 0.05). Compared to the high-temperature groups, in the 0.4 mg/kg chromium-supplemented group, the insulin concentration and insulin resistance index were reduced (p < 0.05), and sodium-glucose transporter 1 expression was up-regulated in jejunum (p < 0.05). Performance, microbiota, gastrointestinal peptides, or serum parameters of the 0.8 mg/kg group were almost unaffected by chromium compared with the high-temperature groups. In conclusion, diet supplemented with 0.4 mg/kg Cr improved performance, insulin resistance and sodium-glucose transporter 1 expression and altered gut microflora structure and secretion of gastrointestinal peptides, thus showing that supplementation with chromium is beneficial to maintain glucose homeostasis and alleviate heat stress.
The Effectiveness of Isoplumbagin and Plumbagin in Regulating Amplitude, Gating Kinetics, and Voltage-Dependent Hysteresis of erg-mediated K+ Currents
Authors: Chen Linyi , Cho Hsin-Yen , Chuang Tzu-Hsien , Ke Ting-Ling , Wu Sheng-Nan
Published: March 2022

Isoplumbagin (isoPLB, 5-hydroxy-3-methyl-1,4-naphthoquinone), a naturally occurring quinone, has been observed to exercise anti-inflammatory, antimicrobial, and antineoplastic activities. Notably, whether and how isoPLB, plumbagin (PLB), or other related compounds impact transmembrane ionic currents is not entirely clear. In this study, during GH3-cell exposure to isoPLB, the peak and sustained components of an erg (ether-à-go-go related gene)-mediated K+ current (IK(erg)) evoked with long-lasting-step hyperpolarization were concentration-dependently decreased, with a concomitant increase in the decaying time constant of the deactivating current. The presence of isoPLB led to a differential reduction in the peak and sustained components of deactivating IK(erg) with effective IC50 values of 18.3 and 2.4 μM, respectively, while the KD value according to the minimum binding scheme was estimated to be 2.58 μM. Inhibition by isoPLB of IK(erg) was not reversed by diazoxide; however, further addition of isoPLB, during the continued exposure to 4,4′-dithiopyridine, did not suppress IK(erg) further. The recovery of IK(erg) by a two-step voltage pulse with a geometric progression was slowed in the presence of isoPLB, and the decaying rate of IK(erg) activated by the envelope-of-tail method was increased in its presence. The strength of the IK(erg) hysteresis in response to an inverted isosceles-triangular ramp pulse was diminished by adding isoPLB. A mild inhibition of the delayed-rectifier K+ current (IK(DR)) produced by the presence of isoPLB was seen in GH3 cells, while minimal changes in the magnitude of the voltage-gated Na+ current were demonstrated in its presence. Moreover, the IK(erg) identified in MA-10 Leydig tumor cells was blocked by adding isoPLB. Therefore, the effects of isoPLB or PLB on ionic currents (e.g., IK(erg) and IK(DR)) demonstrated herein would be upstream of our previously reported perturbations on mitochondrial morphogenesis or respiration. Taken together, the perturbations of ionic currents by isoPLB or PLB demonstrated herein are likely to contribute to the underlying mechanism through which they, or other structurally similar compounds, result in adjustments in the functional activities of different neoplastic cells (e.g., GH3 and MA-10 cells), presuming that similar in vivo observations occur.
Mycobacterium tuberculosis Affects Protein and Lipid Content of Circulating Exosomes in Infected Patients Depending on Tuberculosis Disease State
Authors: Biadglegne Fantahun , Schmidt Johannes R. , Engel Kathrin M. , Lehmann Jörg , Lehmann Robert T. , Reinert Anja , König Brigitte , Schiller Jürgen , Kalkhof Stefan , Sack Ulrich
Published: March 2022

Tuberculosis (TB), which is caused by the bacterium Mycobacterium tuberculosis (Mtb), is still one of the deadliest infectious diseases. Understanding how the host and pathogen interact in active TB will have a significant impact on global TB control efforts. Exosomes are increasingly recognized as a means of cell-to-cell contact and exchange of soluble mediators. In the case of TB, exosomes are released from the bacillus and infected cells. In the present study, a comprehensive lipidomics and proteomics analysis of size exclusion chromatography-isolated plasma-derived exosomes from patients with TB lymphadenitis (TBL) and treated as well as untreated pulmonary TB (PTB) was performed to elucidate the possibility to utilize exosomes in diagnostics and knowledge building. According to our findings, exosome-derived lipids and proteins originate from both the host and Mtb in the plasma of active TB patients. Exosomes from all patients are mostly composed of sphingomyelins (SM), phosphatidylcholines, phosphatidylinositols, free fatty acids, triacylglycerols (TAG), and cholesterylesters. Relative proportions of, e.g., SMs and TAGs, vary depending on the disease or treatment state and could be linked to Mtb pathogenesis and dormancy. We identified three proteins of Mtb origin: DNA-directed RNA polymerase subunit beta (RpoC), Diacyglycerol O-acyltransferase (Rv2285), and Formate hydrogenase (HycE), the latter of which was discovered to be differently expressed in TBL patients. Furthermore, we discovered that Mtb infection alters the host protein composition of circulating exosomes, significantly affecting a total of 37 proteins. All TB patients had low levels of apolipoproteins, as well as the antibacterial proteins cathelicidin, Scavenger Receptor Cysteine Rich Family Member (SSC5D), and Ficolin 3 (FCN3). When compared to healthy controls, the protein profiles of PTB and TBL were substantially linked, with 14 proteins being co-regulated. However, adhesion proteins (integrins, Intercellular adhesion molecule 2 (ICAM2), CD151, Proteoglycan 4 (PRG4)) were shown to be more prevalent in PTB patients, while immunoglobulins, Complement component 1r (C1R), and Glutamate receptor-interacting protein 1 (GRIP1) were found to be more abundant in TBL patients, respectively. This study could confirm findings from previous reports and uncover novel molecular profiles not previously in focus of TB research. However, we applied a minimally invasive sampling and analysis of circulating exosomes in TB patients. Based on the findings given here, future studies into host–pathogen interactions could pave the way for the development of new vaccines and therapies.
Effect of Thickness of InP Nucleation Layer on the Two-Step Growth of InP on Si(001)
Authors: Yan Silu , Lv Hongliang , Zhang Yuming , Yang Shizheng
Published: March 2022

InP nucleation layers with different thicknesses were grown on Si(001) substrates by gas-source molecular beam epitaxy (GSMBE), and the two-step growth technique was used to overcome the large lattice mismatch (8%) between the InP nucleation layer and Si substrate. The surface morphology and microstructure were investigated by using an atomic force microscope (AFM) and transmission electron microscopy (TEM). High-resolution X-ray diffraction (HR-XRD) measurements were carried out to characterize the crystal quality. It was found that a too thin nucleation layer will lead to an uneven distribution of atoms on the surface, resulting in a poor crystalline quality of the InP epitaxial layer. The thicker the low-temperature nucleation layer is, the better the crystallization quality of the InP high-temperature layer will be.
Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran
Authors: Madani Arghavan , Esfandiari Zahra , Shoaei Parisa , Ataei Behrooz
Published: March 2022

Diarrheagenic E. coli (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of 200 samples, including traditional and pasteurized dairy products, were analyzed. In 200 samples, 54 E. coli isolates, including (48/110) and (6/90) positive samples of traditional and pasteurized dairy products, were detected. Furthermore, pathogenic strains were isolated from 30% of traditional dairy products and 5.55% of pasteurized dairy products. Most isolates were classified as enteropathogenic E. coli (EPEC). Moreover, antibiotic resistance was evaluated using the disk diffusion method for pathogenic E. coli. Overall, 73.68% of contaminated samples by pathogenic strains were resistant to at least one antibiotic. The highest resistance was observed against streptomycin (57.9%), followed by tetracycline (50%). Additionally, all isolates were sensitive to amikacin. For evaluating biofilm formation, the violet crystal assay was applied on a polystyrene microplate well for pathogenic isolates. In total, 68.42% of isolates were able to form biofilms. The presence of E. coli in dairy products indicates potential health risks for Iranian consumers. Serious measures are needed to control and prevent the spread of this pathogen.
Raman and near Infrared Spectroscopy for Quantification of Fatty Acids in Muscle Tissue—A Salmon Case Study
Authors: Afseth Nils Kristian , Dankel Katinka , Andersen Petter Vejle , Difford Gareth Frank , Horn Siri Storteig , Sonesson Anna , Hillestad Borghild , Wold Jens Petter , Tengstrand Erik
Published: March 2022

The aim of the present study was to critically evaluate the potential of using NIR and Raman spectroscopy for prediction of fatty acid features and single fatty acids in salmon muscle. The study was based on 618 homogenized salmon muscle samples acquired from Atlantic salmon representing a one year-class nucleus, fed the same high fish oil feed. NIR and Raman spectra were used to make regression models for fatty acid features and single fatty acids measured by gas chromatography. The predictive performance of both NIR and Raman was good for most fatty acids, with R2 above 0.6. Overall, Raman performed marginally better than NIR, and since the Raman models generally required fewer components than respective NIR models to reach high and optimal performance, Raman is likely more robust for measuring fatty acids compared to NIR. The fatty acids of the salmon samples co-varied to a large extent, a feature that was exacerbated by the overlapping peaks in NIR and Raman spectra. Thus, the fatty acid related variation of the spectroscopic data of the present study can be explained by only a few independent principal components. For the Raman spectra, this variation was dominated by functional groups originating from long-chain polyunsaturated FAs like EPA and DHA. By exploring the independent EPA and DHA Raman models, spectral signatures similar to the respective pure fatty acids could be seen. This proves the potential of Raman spectroscopy for single fatty acid prediction in muscle tissue.
Emerging Pattern of Post-COVID-19 Parosmia and Its Effect on Food Perception
Authors: Parker Jane K. , Methven Lisa , Pellegrino Robert , Smith Barry C. , Gane Simon , Kelly Christine E.
Published: March 2022

Olfactory dysfunction is amongst the many symptoms of Long COVID. Whilst most people that experience smell loss post COVID-19 recover their sense of smell and taste within a few weeks, about 10% of cases experience long-term problems, and their smell recovery journey often begins a few months later when everyday items start to smell distorted. This is known as parosmia. The aim of this study was to identify the key food triggers of parosmic distortions and investigate the relationship between distortion and disgust in order to establish the impact of parosmia on diet and quality of life. In this cross-sectional study (n = 727), respondents experiencing smell distortions completed a questionnaire covering aspects of smell loss, parosmia and the associated change in valence of everyday items. There was a significant correlation between strength and disgust (p < 0.0001), and when the selected items were reported as distorted, they were described as either unpleasant or gag-inducing 84% of the time. This change in valence associated with loss of expected pleasure and the presence of strange tastes and burning sensations must certainly lead to changes in eating behaviours and serious longer-term consequences for mental health and quality of life.
Association of Physical Activity and Quality of Life with Work-Related Musculoskeletal Disorders in the UAE Young Adults
Authors: Alseminy Mennatallah Adel Mohamed Mohmoud , Chandrasekaran Baskaran , Bairapareddy Kalyana Chakravarthy
Published: March 2022

Background: Work-related musculoskeletal disorders (WRMSDs) pose threat to the global economy and work productivity. Though growing evidence shows physical activity and quality of life are major determinants for WRMSDs, the association between physical activity and the quality of life among the young adults of the United Arab Emirates (UAE) remains unclear. Methods: In a cross-sectional study, a total of 507 young adults who were between the ages 18–35 years were administered an interviewer-based survey on musculoskeletal disorders, physical activity, and quality of life. The association between the potential determinants and the WRMSDs was analyzed using linear and logistic regression models. Results: High prevalence (75%) of WRMSDs was found among the UAE young adults. Participants with low leisure-time physical activity had few WRMSDs. There was no significant association between physical activity or quality-of-life scores with the incidence of WRMSDs although physical activity time was highly associated with the quality of life, especially the social domain. Conclusion: Though a high prevalence of WRMSDs among UAE men and women was found, neither physical activity nor the quality-of-life scores determined the incidence of WRMSDs.
Association of Physical Fitness, Screen Time, and Sleep Hygiene According to the Waist-to-Height Ratio in Children and Adolescents from the Extreme South of Chile
Authors: Albornoz-Guerrero Javier , Carrasco-Marín Fernanda , Zapata-Lamana Rafael , Cigarroa Igor , Reyes-Molina Daniel , Barceló Olga , García-Pérez-de-Sevilla Guillermo , García-Merino Sonia
Published: March 2022

Objective: To analyze the perception of physical fitness, screen time, and self-reported sleep hygiene in children and adolescents (CA) from the extreme south of Chile and its associations with waist-to-height ratio (WtHr). Material and methods: An observational cross-sectional study was conducted in a sample of 594 schoolchildren from 5th to 8th grade of primary education, belonging to municipal educational establishments in the Magallanes region, Chile. Cardiorespiratory fitness was assessed through the 20-m shuttle run test, muscle strength through handgrip and the standing broad jump test, physical fitness perception through the International Fitness Scale, and central obesity through the waist-to-height index. In addition, sleep hygiene and screen time were measured. Results: More than 92% of CA spent more than two hours a day watching or using screens. In addition, CA with excess central adiposity had a lower perception of physical fitness, and lower muscle strength and cardiorespiratory fitness compared to CA with normal values of adiposity. Conclusions: CA of the present study spent a high number of hours watching or using screens and had poor sleep quality. In addition, excessive central adiposity was associated with lower physical fitness.
Patient Safety Attitudes among Doctors and Nurses: Associations with Workload, Adverse Events, Experience
Authors: AL-Mugheed Khaild , Bayraktar Nurhan , Al-Bsheish Mohammad , AlSyouf Adi , Jarrar Mu’taman , AlBaker Waleed , Aldhmadi Badr K.
Published: March 2022

Patient safety concept has achieved more attention from healthcare organizations to improve the safety culture. This study aimed to investigate patient safety attitudes among doctors and nurses and explore associations between workload, adverse events, and experience with patient safety attitudes. The study used a descriptive cross-sectional design and the Turkish version of the Safety Attitudes Questionnaire. Participants included 73 doctors and 246 nurses working in two private hospitals in Northern Cyprus. The participants had negative perceptions in all patient safety domains. The work conditions domain received the highest positive perception rate, and the safety climate domain received the lowest perception rate among the participants. Nurses showed a higher positive perception than doctors regarding job satisfaction, stress recognition, and perceptions of management domains. There were statistically significant differences between experiences, workloads, adverse events, and total mean scores of patient safety attitudes. Policymakers and directors can improve the quality of care of patients and patient safety by boosting the decision-making of health care providers on several domains of safety attitudes. Patient safety needs to be improved in hospitals through in-service education, management support, and institutional regulations.
Risk Determination versus Risk Perception: A New Model of Reality for Human–Machine Autonomy
Authors: Lawless William
Published: March 2022

We review the progress in developing a science of interdependence applied to the determinations and perceptions of risk for autonomous human–machine systems based on a case study of the Department of Defense’s (DoD) faulty determination of risk in a drone strike in Afghanistan; the DoD’s assessment was rushed, suppressing alternative risk perceptions. We begin by contrasting the lack of success found in a case study from the commercial sphere (Facebook’s use of machine intelligence to find and categorize “hate speech”). Then, after the DoD case study, we draw a comparison with the Department of Energy’s (DOE) mismanagement of its military nuclear wastes that created health risks to the public, DOE employees, and the environment. The DOE recovered by defending its risk determinations and challenging risk perceptions in public. We apply this process to autonomous human–machine systems. The result from this review is a major discovery about the costly suppression of risk perceptions to best determine actual risks, whether for the military, business, or politics. For autonomous systems, we conclude that the determinations of actual risks need to be limited in scope as much as feasible; and that a process of free and open debate needs to be adopted that challenges the risk perceptions arising in situations facing uncertainty as the best, and possibly the only, path forward to a solution.
Acute-Phase Initiation of Cardiac Rehabilitation for Short-Term Improvement in Activities of Daily Living in Patients Hospitalized for Acute Heart Failure
Authors: Ueno Kensuke , Kamiya Kentaro , Kaneko Hidehiro , Okada Akira , Itoh Hidetaka , Fujiu Katsuhito , Takeda Norifumi , Morita Hiroyuki , Michihata Nobuaki , Jo Taisuke , Yasunaga Hideo , Komuro Issei
Published: March 2022

Background: Whether acute-phase cardiac rehabilitation (CR) is beneficial for short-term improvement in activities of daily living (ADL) in patients hospitalized for acute heart failure (AHF) remains unclear. Aim: To investigate the association of acute-phase initiation of CR with short-term improvement in ADL in patients hospitalized for AHF. Methods: We retrospectively analyze data from the Diagnosis Procedure Combination Database, a nationwide inpatient database. Patients hospitalized for HF between January 2010 and March 2018 are included. Propensity score matching and generalized linear models are built to examine the association between improvement in ADL and acute-phase CR initiation, defined as the initiation of CR within two days of admission. Results: Among 306,826 eligible patients, CR is initiated in 45,428 patients (14.8%) within two days of hospital admission. Propensity score matching creates 45,427 pairs. CR initiation within two days of hospital admission is associated with ADL improvement (risk ratio: 1.018; 95% confidence interval: 1.004–1.032), particularly in elderly patients, females, and individuals with low ADL at admission, body mass index of 18.5–24.9 kg/m2, and New York Heart Association class IV. Conclusions: Our analyses highlight the possibility that acute-phase CR initiation may result in short-term improvement in ADL in patients hospitalized for AHF.
Mid-Term Clinical Outcomes Following Drug-Coated Balloons in Coronary Artery Disease
Authors: Sella Gal , Gandelman Gera , Teodorovich Nicholay , Tuvali Ortal , Ayyad Omar , Abu Khadija Haitham , Haberman Dan , Poles Lion , Jonas Michael , Volodarsky Igor , George Jacob , Blatt Alex
Published: March 2022

Objective: The aim of this study was to evaluate the mid-term efficacy of drug-coated balloons (DCB) in percutaneous coronary intervention (PCI) in two different pathophysiologic scenarios. Background: There are different underlying pathological processes in coronary artery disease. Mid-term safety and efficacy of DCB approach is still limited. Methods: Medical records of all consecutive patients undergoing DCB were evaluated. The primary endpoint was the rate of clinically driven target lesion revascularization (TLR) after 24 months. Results: Between January 2011 and December 2017, 442 patients were included, representing 4.4% of all PCIs in our institution. A total of 460 DCB lesions were treated, of which 328 (71.3%) were de novo and 132 (28.7%) were combined bare metal or drug-eluting stents with in-stent restenosis (ISR). The patients’ mean age was 66.2 ± 11.7 years with a diabetes prevalence of 45.3%. The TLR rate was lower in the de novo group (5.3%) compared to the ISR group (9.4%) (p = 0.04). No differences were observed in major adverse cardiovascular events (MACE) between the de novo group (38.9%) and ISR group (42.5%) (p = 0.47). No significant differences were detected in the TLR occurrence in the subgroup analysis. Conclusion: Our extended experience demonstrates that the mid-term DCB approach in these two pathophysiologic settings represent a reasonable option, with low TLR rate.
Impacts of an Altimetric Wave Data Assimilation Scheme and Currents-Wave Coupling in an Operational Wave System: The New Copernicus Marine IBI Wave Forecast Service
Authors: Toledano Cristina , Ghantous Malek , Lorente Pablo , Dalphinet Alice , Aouf Lotfi , Sotillo Marcos G.
Published: March 2022

The Copernicus Marine IBI-MFC (Iberia–Biscay–Ireland Monitoring and Forecasting Centre) has delivered operational wave forecasts since 2017. The operational application is based on a MFWAM model (Meteo-France WAve Model) set-up, running at a 1/20º grid (5-km). The research presented here was conducted to improve the accuracy of the IBI-MFC wave model products, by means of (i) including a new wave data assimilation scheme and (ii) developing a new coupled ocean-wave modelling framework. Evaluation of these set-up upgrades, in terms of improvements in IBI wave model system capabilities, is here presented. All the model sensitivity test runs, performed for the year 2018, are assessed over the whole IBI domain, using the available in-situ (from 49 mooring buoys) and independent satellite wave observation. The results show that the most relevant improvement is due to the data assimilation, while the impact of surface ocean currents, although less significant, also improves the wave model qualification over the IBI area. The demonstrated benefit, related to the herein proposed upgrades, supported the IBI-MFC decision to evolve its operational wave system, using (since the March 2020 Copernicus Marine Release) the resulting wave model set-up, with data assimilation and currents-wave coupling for operational purposes.
Development of Numerical Modelling Techniques for Composite Cylindrical Structures under External Pressure
Authors: Sohn Jung Min , Hirdaris Spyros , Romanoff Jani , Kim Sang Jin
Published: March 2022

Submarine hulls are pressure vessels for which excellent structural integrity under underwater pressure loads is essential. The use of light-weight materials contributes to reduced fuel consumption, improved speed, and increased payload while strength properties are retained. The focus of this paper is on the collapse behavior of a filament-wound cylindrical structure that serves as the main hull of a submarine subject to hydrostatic pressure loads. This paper presents a computational modelling approach for the prediction of the collapse behavior mechanism using a commercial finite element (FE) solver. The collapse strength obtained from the numerical model corresponded closely to available experimental data. The composite and aluminum material models were compared and the effects of stacking angle and thickness portion in the ply sequence on collapse strength were investigated. The advantages and disadvantages of available design codes (i.e., American Society of Mechanical Engineers (ASME) BPVC-X and National Aeronautics and Space Administration (NASA) SP-8007) were reviewed by direct comparison with numerical results. It is concluded that the application of effective engineering constants for the prediction of the collapse pressure of submarine hulls may be feasible.
Constructal Design of an Overtopping Wave Energy Converter Incorporated in a Breakwater
Authors: Martins Jaifer Corrêa , Fragassa Cristiano , Goulart Marcelo Moraes , dos Santos Elizaldo Domingues , Isoldi Liércio André , das Neves Gomes Mateus , Rocha Luiz Alberto Oliveira
Published: March 2022

A numerical study was performed in the present work about an Overtopping Device Wave Energy Converter (OTD-WEC) with one and two ramps incorporated in a real breakwater. The Constructal Design method was applied to evaluate the effects on the average dimensionless overtopping flow of the degrees of freedom of the device with one and two ramps. In addition, a comparison was carried out among the different geometry configurations of the OTD-WEC to determine which one presents the best hydrodynamic performance. The work used the JONSWAP spectrum and the multiphase Volume of Fluid model. It also solved the conservation equations for mass, momentum, and an equation for the transport of the volume fraction using the Finite Volume Method. Results showed that a device with a two-ramps configuration presented an average dimensionless overtopping flow 6.48% higher than those obtained for the one ramp. Present results obtained using Constructal Design theoretical recommendations about the influence of a complex configuration with four degrees of freedom over the performance of an OTD-WEC integrated into the east breakwater of the city of São José do Norte, State of Rio Grande do Sul (RS), Brazil.
Experimental and Statistical Study of the Fracture Mechanism of Sn96.5Ag3Cu0.5 Solder Joints via Ball Shear Test
Authors: Liang Kun , Wang Yuexing , He Zhigang
Published: March 2022

Ball shear testing is an efficient approach to investigate the mechanical reliability of solder joints at the structural level. In the present study, a series of low-speed ball shear tests were conducted to study the deformation and fracture characteristics of Sn96.5Ag3Cu0.5 solder joints at continuous speeds from 10 μm/s to 200 μm/s. In order to account for randomness, the quantity of tests was repeated for each shear rate. The relationship between mechanical properties and shear speeds was calculated in detail via effective statistical analysis. In addition, by utilizing SEM imaging and ingredient analysis the interfacial effect and fracture mechanism of solder balls were obtained and their fracture mode classified into two types, viz., bulk fracture and interface fracture. Furthermore, by means of statistical analysis and approximate calculation it was proven that bulk fracture balls have greater adhesive powers and reliability compared with interface fracture balls.
Scale and Background Aware Asymmetric Bilateral Network for Unconstrained Image Crowd Counting
Authors: Lv Gang , Xu Yushan , Ma Zuchang , Sun Yining , Nian Fudong
Published: March 2022

This paper attacks the two challenging problems of image-based crowd counting, that is, scale variation and complex background. To that end, we present a novel crowd counting method, called the Scale and Background aware Asymmetric Bilateral Network (SBAB-Net), which is able to handle scale variation and background noise in a unified framework. Specifically, the proposed SBAB-Net contains three main components, a pre-trained backbone convolutional neural network (CNN) as the feature extractor and two asymmetric branches to generate a density map. These two asymmetric branches have different structures and use features from different semantic layers. One branch is densely connected stacked dilated convolution (DCSDC) sub-network with different dilation rates, which relies on one deep feature layer and can handle scale variation. The other branch is parameter-free densely connected stacked pooling (DCSP) sub-network with various pooling kernels and strides, which relies on shallow feature and can fuse features with several receptive fields to reduce the impact of background noise. Two sub-networks are fused by the attention mechanism to generate the final density map. Extensive experimental results on three widely-used benchmark datasets have demonstrated the effectiveness and superiority of our proposed method: (1) We achieve competitive counting performance compared to state-of-the-art methods; (2) Compared with baseline, the MAE and MSE are decreased by at least 6.3% and 11.3%, respectively.
Complexity of Solutions Combination for the Three-Index Axial Assignment Problem
Authors: Afraimovich Lev G. , Emelin Maxim D.
Published: March 2022

In this work we consider the NP-hard three-index axial assignment problem. We formulate and investigate a problem of combining feasible solutions. Such combination can be applied in a wide range of heuristic and approximate algorithms for solving the assignment problem, instead of the commonly used strategy of selecting the best solution among the found feasible solutions. We discuss approaches to a solution of the combination problem and prove that it becomes NP-hard already in the case of combining four solutions.
Anomalous Areas Detection in Rocks Using Time-Difference Adjoint Tomography
Authors: Wang Feiyue , Xie Xin , Pei Zhongwei , Dong Longjun
Published: March 2022

Detecting anomalous areas (such as caves, faults, and weathered layers) in rocks is essential for the safety of facilities and personnel in subsurface engineering. Seismic tomography has been proved to be an effective exploration technology in engineering geophysics. However, the complexity, anisotropy, and uncertainty in rock environments pose challenges to the resolution and robustness of tomography methods. Traditional tomography methods have difficulty balancing reliability and efficiency. Therefore, we developed a time-difference adjoint tomography method combining the arrival-time difference and the adjoint state method. The effectiveness was verified by numerical experiments and a laboratory-scale acoustic experiment. The effectiveness of the proposed method was demonstrated by the experimental results. The adjoint scheme avoids additional ray tracing and improves the efficiency of the inversion, which allows the use of finer forward grids in practice. By considering the differential arrivals of receiver pairs, the proposed method is robust in the face of systematic errors and relatively stable against large random noises. Moreover, the velocity contrast obtained by the proposed method is sharper than for first-arrival tomography in the areas where the rays are not dense, resulting in a clearer indication of the anomalous areas in the tomographic image.
First Record of Hepatozoon spp. in Alpine Wild Rodents: Implications and Perspectives for Transmission Dynamics across the Food Web
Authors: Ferrari Giulia , Girardi Matteo , Cagnacci Francesca , Devineau Olivier , Tagliapietra Valentina
Published: March 2022

Among the Apicomplexa parasites, Hepatozoon spp. have been mainly studied in domestic animals and peri-urban areas. The epidemiology of Hepatozoon spp. is poorly investigated in natural systems and wild hosts because of their scarce veterinary and economic relevance. For most habitats, the occurrence of these parasites is unknown, despite their high ecosystemic role. To fill this gap for alpine small mammals, we applied molecular PCR-based methods and sequencing to determine the Hepatozoon spp. in 830 ear samples from 11 small mammal species (i.e., Apodemus, Myodes, Chionomys, Microtus, Crocidura and Sorex genera) live-trapped during a cross-sectional study along an altitudinal gradient in the North-Eastern Italian Alps. We detected Hepatozoon spp. with an overall prevalence of 35.9%. Two species ranging from 500 m a.s.l. to 2500 m a.s.l. were the most infected: My. glareolus, followed by Apodemus spp. Additionally, we detected the parasite for the first time in another alpine species: C. nivalis at 2000–2500 m a.s.l. Our findings suggest that several rodent species maintain Hepatozoon spp. along the alpine altitudinal gradient of habitats. The transmission pathway of this group of parasites and their role within the alpine mammal community need further investigation, especially in consideration of the rapidly occurring environmental and climatic changes.
Identification and Characterization of Marine Microorganisms by Tandem Mass Spectrometry Proteotyping
Authors: Lozano Clément , Kielbasa Mélodie , Gaillard Jean-Charles , Miotello Guylaine , Pible Olivier , Armengaud Jean
Published: March 2022

The vast majority of marine microorganisms and their functions are yet to be explored. The considerable diversity they encompass is an endless source of knowledge and wealth that can be valued on an industrial scale, emphasizing the need to develop rapid and efficient identification and characterization techniques. In this study, we identified 26 microbial isolates from coastal water of the NW Mediterranean Sea, using phylopeptidomics, a cutting-edge tandem mass spectrometry proteotyping technique. Taxonomical identification at the species level was successfully conducted for all isolates. The presence of strains belonging to the newly described Balneolaeota phylum, yet uncharacterized at the proteomics scale, was noted. The very first proteomics-based investigation of a representative of the Balneolaeota phylum, Balneola vulgaris, is proposed, demonstrating the use of our proteotyping workflow for the rapid identification and in-depth molecular characterization, in a single MS/MS analytical run. Tandem mass spectrometry proteotyping is a valuable asset for culturomic programs as the methodology is able to quickly classify the most atypical isolates.
Antifungal Sesquiterpenoids from Michelia formosana Leaf Essential Oil against Wood-Rotting Fungi
Authors: Wu Chia-Che , Huang Shou-Ling , Ko Chun-Han , Chang Hui-Ting
Published: March 2022

Michelia formosana (Kanehira) Masamune is a broad-leaved species widespread in East Asia; the wood extract and its constituents possess antifungal activity against wood-decay fungi. Antifungal activities of leaf essential oil and its constituents from M. formosana were investigated in the present study. Bioassay-guided isolation was applied to isolate the phytochemicals from leaf essential oil. 1D and 2D NMR, FTIR, and MS spectroscopic analyses were applied to elucidate the chemical structures of isolated compounds. Leaf essential oil displayed antifungal activity against wood decay fungi and was further separated into 11 fractions by column chromatography. Four sesquiterpenoids were isolated and identified from the active fractions of leaf essential oil through bioassay-guided isolation. Among these sesquiterpenoids, guaiol, bulnesol, and β-elemol have higher antifungal activity against brown-rot fungus Laetiporus sulphureus and white-rot fungus Lenzites betulina. Leaf essential oil and active compounds showed better antifungal activity against L. sulphureus than against L. betulina. The molecular structure of active sesquiterpenoids all contain the hydroxyisopropyl group. Antifungal sesquiterpenoids from M. formosana leaf essential oil show potential as natural fungicides for decay control of lignocellulosic materials.