bluuebunny/crossref_metadata_2025 huggingface dataset:
Applying a Deep Learning Model for Total Kidney Volume Measurement in Autosomal Dominant Polycystic Kidney Disease
Authors: Hsu Jia-Lien , Singaravelan Anandakumar , Lai Chih-Yun , Li Zhi-Lin , Lin Chia-Nan , Wu Wen-Shuo , Kao Tze-Wah , Chu Pei-Lun
Published: September 2024

Background: Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary renal disease leading to end-stage renal disease. Total kidney volume (TKV) measurement has been considered as a surrogate in the evaluation of disease severity and prognostic predictor of ADPKD. However, the traditional manual measurement of TKV by medical professionals is labor-intensive, time-consuming, and human error prone. Materials and methods: In this investigation, we conducted TKV measurements utilizing magnetic resonance imaging (MRI) data. The dataset consisted of 30 patients with ADPKD and 10 healthy individuals. To calculate TKV, we trained models using both coronal- and axial-section MRI images. The process involved extracting images in Digital Imaging and Communications in Medicine (DICOM) format, followed by augmentation and labeling. We employed a U-net model for image segmentation, generating mask images of the target areas. Subsequent post-processing steps and TKV estimation were performed based on the outputs obtained from these mask images. Results: The average TKV, as assessed by medical professionals from the testing dataset, was 1501.84 ± 965.85 mL with axial-section images and 1740.31 ± 1172.21 mL with coronal-section images, respectively (p = 0.73). Utilizing the deep learning model, the mean TKV derived from axial- and coronal-section images was 1536.33 ± 958.68 mL and 1636.25 ± 964.67 mL, respectively (p = 0.85). The discrepancy in mean TKV between medical professionals and the deep learning model was 44.23 ± 58.69 mL with axial-section images (p = 0.8) and 329.12 ± 352.56 mL with coronal-section images (p = 0.9), respectively. The average variability in TKV measurement was 21.6% with the coronal-section model and 3.95% with the axial-section model. The axial-section model demonstrated a mean Dice Similarity Coefficient (DSC) of 0.89 ± 0.27 and an average patient-wise Jaccard coefficient of 0.86 ± 0.27, while the mean DSC and Jaccard coefficient of the coronal-section model were 0.82 ± 0.29 and 0.77 ± 0.31, respectively. Conclusion: The integration of deep learning into image processing and interpretation is becoming increasingly prevalent in clinical practice. In our pilot study, we conducted a comparative analysis of the performance of a deep learning model alongside corresponding axial- and coronal-section models, a comparison that has been less explored in prior research. Our findings suggest that our deep learning model for TKV measurement performs comparably to medical professionals. However, we observed that varying image orientations could introduce measurement bias. Specifically, our AI model exhibited superior performance with axial-section images compared to coronal-section images.
Investigating the Interplay between Cardiovascular and Neurodegenerative Disease
Authors: Cousineau Jason Patrick , Dawe Aimee Maria , Alpaugh Melanie
Published: September 2024

Neurological diseases, including neurodegenerative diseases (NDDs), are the primary cause of disability worldwide and the second leading cause of death. The chronic nature of these conditions and the lack of disease-modifying therapies highlight the urgent need for developing effective therapies. To accomplish this, effective models of NDDs are required to increase our understanding of underlying pathophysiology and for evaluating treatment efficacy. Traditionally, models of NDDs have focused on the central nervous system (CNS). However, evidence points to a relationship between systemic factors and the development of NDDs. Cardiovascular disease and related risk factors have been shown to modify the cerebral vasculature and the risk of developing Alzheimer’s disease. These findings, combined with reports of changes to vascular density and blood–brain barrier integrity in other NDDs, such as Huntington’s disease and Parkinson’s disease, suggest that cardiovascular health may be predictive of brain function. To evaluate this, we explore evidence for disruptions to the circulatory system in murine models of NDDs, evidence of disruptions to the CNS in cardiovascular disease models and summarize models combining cardiovascular disruption with models of NDDs. In this study, we aim to increase our understanding of cardiovascular disease and neurodegeneration interactions across multiple disease states and evaluate the utility of combining model systems.
Evaluation of Cyfluthrin and Etofenprox Resistance in House Fly Musca domestica Populations in Antalya, Türkiye
Authors: Oz Emre
Published: September 2024

The house fly, Musca domestica L. (Diptera: Muscidae), is a significant vector for many pathogens and parasites. Presence of this vector causes economic losses due to decreased feeding activity in livestock, resulting in reduced yields of products. The repeated and unconscious application of chemical insecticides has resulted in the development of resistance in the majority of house fly populations worldwide. The aim of this research was to determine the susceptibility levels of two field M. domestica populations to cyfluthrin and etofenprox in Antalya, Türkiye. The LD50 values, resistance ratios, and resistance status were determined by exposing adult house flies to the test chemicals for one hour. The Kemer population exhibited very low resistance to cyfluthrin, with a 5-fold increase, while the Serik population, in contrast, showed moderate resistance with a 29.67-fold increase. The resistance ratios of field populations to etofenprox were 2.33-fold (no resistance) for Kemer and 6.44-fold (very low resistance) for Serik, respectively. This is the first study to determine the resistance levels of house flies against cyfluthrin and etofenprox insecticides in Türkiye. To prevent or reduce the development of resistance to insecticides in house flies, integrated control methods and resistance management programs should be employed. Furthermore, it is advised that regular monitoring tests be conducted to observe the level of resistance.
Endemic Radiation of African Moonfish, Selene dorsalis (Gill 1863), in the Eastern Atlantic: Mitogenomic Characterization and Phylogenetic Implications of Carangids (Teleostei: Carangiformes)
Authors: Ewusi Emmanuel Ofosu Mireku , Lee Soo Rin , Kim Ah Ran , Go Yunji , Htoo Hsu , Chung Sangdeok , Amin Muhammad Hilman Fu’adil , Andriyono Sapto , Kim Hyun-Woo , Kundu Shantanu
Published: September 2024

This study offers an in-depth analysis of the mitochondrial genome of Selene dorsalis (Gill 1863), a species native to the Eastern Atlantic Ocean. The circular mitochondrial DNA molecule measures 16,541 base pairs and comprises 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes, and a control region (CR). The nucleotide composition exhibits a notable adenine-thymine (AT) bias, accounting for 53.13%, which aligns with other species in the Carangidae family. Most PCGs initiate with the ATG codon, with the exception of Cytochrome C oxidase subunit I, which starts with GTG. Analysis of relative synonymous codon usage reveals that leucine and serine are the most prevalent amino acids in the mitochondrial genome of S. dorsalis and its congeners (S. vomer and S. setapinnis). All tRNAs display the typical cloverleaf structure, though tRNA Serine (S1) lacks a dihydrouracil arm. Pairwise comparisons of synonymous and nonsynonymous substitutions for all PCGs yielded values below ‘1’, indicating strong purifying selection. The CR spans 847 bp, representing 5.12% of the mitochondrial genome, and is characterized by high AT content (62.81%). It is situated between tRNA-Pro (TGG) and tRNA-Phe (GAA). The CR contains conserved sequence blocks, with CSB-1 being the longest at 22 bp and CSB-D the shortest at 18 bp. Phylogenetic analysis, using Bayesian and Maximum-likelihood trees constructed from concatenated PCGs across 72 species, successfully differentiates S. dorsalis from other carangids. This study also explores how ocean currents and gyres might influence lineage diversification and parapatric speciation of Selene species between the Atlantic and Pacific Oceans. These results highlight the importance of the mitochondrial genome in elucidating the structural organization and evolutionary dynamics of S. dorsalis and its relatives within marine ecosystems.
Immunoglobulin G and Complement as Major Players in the Neurodegeneration of Multiple Sclerosis
Authors: Kennedy Peter G. E. , Fultz Matthew , Phares Jeremiah , Yu Xiaoli
Published: September 2024

Multiple Sclerosis (MS) is an inflammatory, demyelinating, and neurodegenerative disease of the central nervous system (CNS) and is termed as one of the most common causes of neurological disability in young adults. Axonal loss and neuronal cell damage are the primary causes of disease progression and disability. Yet, little is known about the mechanism of neurodegeneration in the disease, a limitation that impairs the development of more effective treatments for progressive MS. MS is characterized by the presence of oligoclonal bands and raised levels of immunoglobulins in the CNS. The role of complement in the demyelinating process has been detected in both experimental animal models of MS and within the CNS of affected MS patients. Furthermore, both IgG antibodies and complement activation can be detected in the demyelinating plaques and cortical gray matter lesions. We propose here that both immunoglobulins and complement play an active role in the neurodegenerative process of MS. We hypothesize that the increased CNS IgG antibodies form IgG aggregates and bind complement C1q with high affinity, activating the classical complement pathway. This results in neuronal cell damage, which leads to neurodegeneration and demyelination in MS.
The Imbalance of Homocysteine, Vitamin B12 and Folic Acid in Parkinson Plus Syndromes: A Review beyond Parkinson Disease
Authors: Poulidou Vasiliki , Liampas Ioannis , Arnaoutoglou Marianthi , Dardiotis Efthimios , Siokas Vasileios
Published: September 2024

While there is a link between homocysteine (Hcy), B12 and folic acid and neurodegeneration, especially in disorders like Parkinson’s and Alzheimer’s diseases, its role in Parkinson plus syndromes (PPS) has only been partially investigated. It appears that elevated Hcy, along with an imbalance of its essential vitamin cofactors, are both implicated in the development and progression of parkinsonian syndromes, which represent different disease pathologies, namely alpha-synucleinopathies and tauopathies. Attributing a potential pathogenetic role in hyperhomocysteinemia would be crucial in terms of improving the diagnostic and prognostic accuracy of these syndromes and also for providing a new target for possible therapeutic intervention. The scope of this review is to focus on vitamin imbalance in PPS, with a special emphasis on the role of Hcy, B12 and folic acid in the neurodegenerative process and their implication in the therapeutic approach of these disorders.
Effects of Hyaluronic Acid (HA) and Platelet-Rich Plasma (PRP) on Mandibular Mobility in Temporomandibular Joint Disorders: A Controlled Clinical Trial
Authors: Chęciński Maciej , Chlubek Dariusz , Sikora Maciej
Published: September 2024

Hyaluronic acid (HA) is a glycosaminoglycan composed of D-glucuronic acid and N-acetylglucosamine with an up-to-several-million-Daltons chain-length responsible for the lubricating properties of the temporomandibular joint (TMJ) synovial fluid. Arthritis results in the predominance of HA degradation over synthesis leading to temporomandibular disorders (TMDs). TMD injection treatments are divided into HA supplementation and platelet-rich plasma (PRP) inflammation suppression. We questioned whether either approach lubricated the TMJ better and answered it in a two-arm equal-allocation trial with a non-concurrent active treatment control (two groups of 39 patients each). HA statistically significantly improved (p < 0.01) and PRP did not statistically significantly change (0.06 ≤ p ≤ 0.53) articular mobility compared to baselines in 128 TMJs. Statistically significant inter-group discrepancies were observed for abduction (MD = −4.05 mm; SE = 1.08; p = 0.00; d = −0.85) and protrusion (MD = −0.97 mm; SE = 0.43; p = 0.03; d = −0.51) but not for rightward (MD = −0.21; SE = 0.43; p = 0.63; d = −0.11) and leftward (MD = −0.30; SE = 0.42; p = 0.47; d = −0.16) movements. HA supplementation proved superior to PRP autografting in ad hoc TMJ lubrication and hence is more appropriate in hypomobile TMD cases of symptomatic treatment.
The Internalization Pathways of Liposomes, PLGA, and Magnetic Nanoparticles in Neutrophils
Authors: Garanina Anastasiia , Vishnevskiy Daniil , Chernysheva Anastasia , Malinovskaya Julia , Lazareva Polina , Semkina Alevtina , Abakumov Maxim , Naumenko Victor
Published: September 2024

Background/Objectives: Neutrophils are emerging as promising candidates for cell-based nanodrug delivery to tumors due to their unique biological properties. This study aims to investigate the mechanisms of nanoparticle internalization by neutrophils, specifically focusing on liposomes, poly(lactic-co-glycolic acid) (PLGA), and magnetite nanoparticles. Understanding these mechanisms could enhance the efficiency of neutrophil-based nanodrug delivery for cancer treatment. Methods: Neutrophils were isolated from the peripheral blood of mice bearing 4T1 mammary adenocarcinoma. Confocal microscopy, transmission electron microscopy, and flow cytometry were employed to evaluate the uptake of liposomes, PLGA, and magnetite nanoparticles by neutrophils. The effects of cultivation conditions, such as the presence or absence of plasma in the growth medium, were also examined. Additionally, the roles of immunoglobulins (IgG/IgM) and cell surface receptors (Fc and scavenger receptors) in nanoparticle internalization were explored. Results: All types of nanoparticles were successfully internalized by neutrophils, though the mechanisms of uptake varied. Plasma presence in the medium significantly influenced nanoparticle binding, particularly for PLGA nanoparticles. Internalization of PLGA nanoparticles was found to depend on the presence of IgG/IgM in the medium and Fc receptors on neutrophil surfaces, while scavenger receptors were not involved. Conclusions: Understanding the distinct endocytosis pathways for different nanoparticles can improve the efficacy of neutrophil loading with nanodrugs, potentially advancing the development of neutrophil-based cancer therapies. The findings underscore the importance of the extracellular environment in modulating nanoparticle uptake.
Investigating the Epigenetic Landscape of Major Depressive Disorder: A Genome-Wide Meta-Analysis of DNA Methylation Data, Including New Insights into Stochastic Epigenetic Mutations and Epivariations
Authors: Baldrighi Giulia Nicole , Cavagnola Rebecca , Calzari Luciano , Sacco Davide , Costantino Lucy , Ferrara Fulvio , Gentilini Davide
Published: September 2024

Background/Objectives: Major depressive disorder (MDD) is a mental health condition that can severely impact patients’ social lives, leading to withdrawal and difficulty in maintaining relationships. Environmental factors such as trauma and stress can worsen MDD by interacting with genetic predispositions. Epigenetics, which examines changes in gene expression influenced by the environment, may help identify patterns linked to depression. This study aimed to explore the epigenetic mechanisms behind MDD by analysing six public datasets (n = 1125 MDD cases, 398 controls in blood; n = 95 MDD cases, 96 controls in brain tissues) from the Gene Expression Omnibus. Methods: As an innovative approach, two meta-analyses of DNA methylation patterns were conducted alongside an investigation of stochastic epigenetic mutations (SEMs), epigenetic age acceleration, and rare epivariations. Results: While no significant global methylation differences were observed between MDD cases and controls, hypomethylation near the SHF gene (brain-specific probe cg25801113) was consistently found in MDD cases. SEMs revealed a gene-level burden in MDD, though epigenetic age acceleration was not central to the disorder. Additionally, 51 rare epivariations were identified in blood tissue and 1 in brain tissue linked to MDD. Conclusions: The study emphasises the potential role of rare epivariations in MDD’s epigenetic regulation but calls for further research with larger, more diverse cohorts to confirm these findings.
Melatonin Improves Vasogenic Edema via Inhibition to Water Channel Aquaporin-4 (AQP4) and Metalloproteinase-9 (MMP-9) Following Permanent Focal Cerebral Ischemia
Authors: Lee Ai-Hua , Tai Shih-Huang , Huang Sheng-Yang , Chang Li-Der , Chen Liang-Yi , Chen Yu-Ning , Hsu Hao-Hsiang , Lee E-Jian
Published: September 2024

Background: The efficacy of melatonin in reducing vasogenic and cytotoxic edema was investigated using a model of permanent middle cerebral artery occlusion (pMCAO). Methods: Rats underwent pMCAO, followed by intravenous administration of either melatonin (5 mg/kg) or a vehicle 10 min post-insult. Brain infarction and edema were assessed, and Western blot analyses were conducted to examine the expression levels of aquaporin-4 (AQP4), metalloproteinase-9 (MMP-9), and the neurovascular tight-junction protein ZO-1 upon sacrifice. The permeability of the blood–brain barrier (BBB) was measured using spectrophotometric quantification of Evans blue dye leakage. Results: Compared to controls, melatonin-treated rats exhibited a significant reduction in infarct volume by 26.9% and showed improved neurobehavioral outcomes (p < 0.05 for both). Melatonin treatment also led to decreased Evans blue dye extravasation and brain edema (p < 0.05 for both), along with lower expression levels of AQP4 and MMP-9 proteins and better preservation of ZO-1 protein (p < 0.05 for all). Conclusions: Therefore, melatonin offers neuroprotection against brain swelling induced by ischemia, possibly through its modulation of AQP4 and MMP-9 activities in glial cells and the extracellular matrix (ECM) during the early phase of ischemic injury.
Editorial for the Special Issue “Gut Dysbiosis: Molecular Mechanisms and Therapies 2.0”
Authors: Stolfi Carmine , Laudisi Federica
Published: September 2024

Gut homeostasis depends on maintaining a fine equilibrium between the intestinal epithelial barrier, the microbiota, and the host’s immune system [...]
MPO-DNA Complexes and cf-DNA in Patients with Sepsis and Their Clinical Value
Authors: Zhang Danmei , Guo Jin , Shi Chunxia , Wang Yukun , Zhang Yanqiong , Zhang Xiaoya , Gong Zuojiong
Published: September 2024

Background/Objectives: Neutrophils, as the first line of defense in the immune response, produce neutrophil extracellular traps (NETs) upon activation, which are significant in the pathogenesis and organ damage in sepsis. This study aims to explore the clinical value of myeloperoxidase-DNA (MPO-DNA) and cell-free DNA (cf-DNA) in sepsis patients. Methods: Clinical data were collected from 106 sepsis patients, 25 non-sepsis patients, and 51 healthy controls. Sequential Organ Failure Assessment (SOFA) scores were calculated, and levels of MPO-DNA) complexes and cf-DNA were measured using specific kits. Correlation analyses assessed relationships between indicators, while logistic regression identified independent risk factors. Receiver operating characteristic (ROC) curves calculated the area under the curve (AUC) to evaluate the diagnostic value of the biomarkers. Results: Sepsis patients exhibited significantly elevated levels of MPO-DNA and cf-DNA compared to non-sepsis patients and healthy controls. In sepsis patients, MPO-DNA and cf-DNA levels correlated with inflammation, coagulation, and organ damage indicators, as well as procalcitonin (PCT) levels and SOFA scores. Both C-reactive protein (CRP) and cf-DNA were identified as independent risk factors for sepsis, demonstrating moderate diagnostic value. ROC analysis showed that the combination of MPO-DNA and CRP (AUC: 0.837) enhances the AUC value of CRP (0.777). Conclusions: In summary, elevated serum levels of MPO-DNA and cf-DNA in sepsis patients correlate with SOFA scores and PCT levels, providing reference value for sepsis diagnosis in clinical settings.
Food Addiction
Authors: Krupa Haley , Gearhardt Ashley N. , Lewandowski Anne , Avena Nicole M.
Published: September 2024

In this review, we aim to draw a connection between drug addiction and overconsumption of highly palatable food (OHPF) by discussing common behaviors and neurochemical pathways shared by these two states. OHPF can stimulate reward pathways in the brain that parallel those triggered by drug use, increasing the risk of dependency. Behavioral similarities between food and drug addiction can be addressed by tracking their stages: loss of control when eating (bingeing), withdrawal, craving, sensitization, and cross-sensitization. The brain adapts to addiction by way of the mesolimbic dopamine system, endogenous opioids and receptors, acetylcholine and dopamine balance, and adaptations of serotonin in neuroanatomy. Studies from the current literature are reviewed to determine how various neurological chemicals contribute to the reinforcement of drug addiction and OHPF. Finally, protocols for treating food addiction are discussed, including both clinical and pharmacological modalities. There is consistent evidence that OHPF changes brain chemistry and leads to addiction in similar ways to drugs. However, more long-term research is needed on food addiction, binge eating, and their neurobiological effects.
From the Bio-Psycho-Social Model to the Development of a Clinical–Forensic Assessment Tool for Chronic Pain in Victims of Violence: A Research Protocol
Authors: Uvelli Allison , Pugliese Erica , Masti Alessandra , Salvo Giulia , Duranti Cristina , Gualtieri Giacomo , Ferretti Fabio
Published: September 2024

Violence against women impacts a minimum of 35% of the global female population, encompassing sexual, physical, and psychological forms. Perpetrators of this violence include partners, family members, or strangers. Its ramifications are substantial, evident in the prevalence of chronic pain reported by between 48% and 84% of women who have experienced abuse, with an odds ratio of 2.08. Notably associated diagnoses include pelvic/vaginal pain, fibromyalgia, irritable bowel syndrome/bowel symptoms, abdominal pain, migraine/headache, and back and neck pain. These diagnoses significantly limit a woman’s ability to participate in daily activities, such as exercising or working, leading to genuine disability. Despite substantial evidence, the precise cause and etiology of these conditions remain unclear. Adhering to the bio-psycho-social model, it is conceivable that chronic pain in victims of violence cannot be attributed to a single factor alone, but rather to a combination of all three: biological, psychological, and social factors. Uncovering these factors could have significant clinical and legal implications. On one hand, it would be possible to conduct screenings to avoid developing chronic pain. and guide individuals toward the correct treatment. On the other hand, victims could seek compensation for chronic pain resulting from violence. Considering the limited knowledge about the causes of chronic pain and the absence of tools to identify risk factors or a set of tests for evaluating victims of violence, the goal of the research described in this project protocol is to pinpoint the specific contributing factors for chronic pain due to violence victimization. Additionally, it aims to devise a comprehensive protocol for assessing these factors in forensic science.
Dissociable Effects of Endogenous and Exogenous Attention on Crowding: Evidence from Event-Related Potentials
Authors: Gong Mingliang , Liu Tingyu , Chen Yingbing , Sun Yingying
Published: September 2024

Background/Objectives: Crowding is a common visual phenomenon that can significantly impair the recognition of objects in peripheral vision. Two recent behavioral studies have revealed that both exogenous and endogenous attention can alleviate crowding, but exogenous attention seems to be more effective. Methods: The present study employed the event-related potential (ERP) technique to explore the electrophysiological characteristics of the influence of these two types of attention on crowding. In the experiment, participants were required to judge whether the letter “T” was upright or inverted, which may be preceded by an exogenous cue or an endogenous cue indicating the location of the target letter. Results: The behavioral results showed that while exogenous cues reduced crowding in all stimulus onset asynchronies (SOAs), endogenous attention took effects only in long SOA. The ERP results indicated that both endogenous and exogenous cues significantly alleviated the inhibition of visual crowding on the N1 component. However, the endogenous cue was effective only under long SOA, while the exogenous cue was effective only under short SOA conditions. In addition, invalid exogenous cues induced a larger P3 wave amplitude than valid ones in the short SOA condition, but endogenous attention did not show such a difference. Conclusions: These results indicate that both endogenous and exogenous attention can alleviate the effects of visual crowding, but they differ in effect size and temporal dynamics.
Cerebrospinal Fluid Neurofilaments Light-Chain Differentiate Patients Affected by Alzheimer’s Disease with Different Rate of Progression (RoP): A Preliminary Study
Authors: Blandino Valeria , Colletti Tiziana , Ribisi Paolo , Tarantino Domenico , Mosca Viviana , Agnello Luisa , Ciaccio Marcello , Piccoli Tommaso
Published: September 2024

Alzheimer’s disease (AD) is the most common neurodegenerative disorder and a leading cause of dementia. One major challenge for clinicians is accurately assessing the rate of disease progression (RoP) early in the diagnostic process, which is crucial for patient management and clinical trial stratification. This study evaluated the role of cerebrospinal fluid biomarkers—Aβ42, t-Tau, pTau, Neurogranin (Ng), and Neurofilament light-chain (NF-L)—in predicting RoP at the time of AD diagnosis. We included 56 AD patients and monitored cognitive impairment using MMSE scores at diagnosis and during six-month follow-up visits. RoP scores were calculated based on these assessments. Our correlation analyses revealed significant associations between RoP and pTau, Aβ42/Ng ratio, and NF-L levels. When patients were stratified by median RoP values into low-to-moderate (L-M: <2) and upper-moderate (U-M: >2) groups, those in the U-M group had notably higher CSF NF-L levels compared to the L-M group. Logistic regression analysis further demonstrated that elevated CSF NF-L levels were predictive of a faster RoP. These findings highlight the potential of CSF NF-L as a prognostic biomarker for rapid disease progression in AD. By identifying patients at risk for accelerated cognitive decline, CSF NF-L could significantly enhance early intervention strategies and improve patient management in clinical settings.
The Impact of Cues on Joint Attention in Children with Autism Spectrum Disorder: An Eye-Tracking Study in Virtual Games
Authors: Liu Lili , Li Shuang , Tian Lin , Yao Xinyu , Ling Yutao , Chen Jingying , Wang Guangshuai , Yang Yang
Published: September 2024

Joint attention (JA), a core deficit in children with autism spectrum disorder (ASD), is crucial for social interaction, emotional understanding, and cognitive development. This study aims to compare and analyze the eye-tracking data of ASD and typically developing children (TDC) during virtual games, exploring how different cue types affect JA performance in ASD children. A total of 31 TDC and 40 ASD children participated in the study. Using eye-tracking devices, we recorded the children’s eye movements as they played virtual games, selecting the correct target based on cues provided by virtual characters. Our findings revealed that different cue types significantly impacted the game scores of ASD children but had no significant effect on TDC, highlighting a notable disparity between the two groups. ASD children showed a lower fixation frequency, irregular fixation paths, and increased attention to non-target objects compared to TDC. Interestingly, among the three cue types, ASD children exhibited a preference for the third type, leading to longer fixation on the region of interest and higher game scores. These results underscore the importance of cue selection in enhancing JA in ASD children. This study provides novel insights into the JA deficits in ASD children and offers a scientific basis for the development of targeted and individualized intervention programs.
Improved Projection Pursuit Model to Evaluate the Maturity of Healthy Building Technology in China
Authors: Zhou Peng , Peng Chenyang , Gan Bin , Wang Zhou , Liu Xueren
Published: September 2024

The development of healthy building technology has become a major trend in the global construction industry, especially in China, owing to accelerating urbanization and increasing health awareness among residents. However, an effective evaluation framework to quantify and evaluate the maturity of healthy building technology is lacking. This paper proposes a novel maturity evaluation model for healthy building technology. After analyzing the Driver–Pressure–State–Impact–Response (DPSIR) framework for asserting the maturity of healthy building in China, it constructs an evaluation indicator system, comprising five and twenty-seven first- and second-class indicators, respectively. Subsequently, this paper constructs an improved projection pursuit model based on border collie optimization. The model obtains evaluation results by mining evaluation data, thus overcoming the limitations of traditional evaluation models in dealing with complex data. The empirical research results demonstrate that China is in the optimization stage in terms of the level of maturity of healthy building technology. The weight of impact is as high as 0.2743, which is the most important first-level indicator. Strict green energy utilization policy requirements are the most important secondary indicator, with a weight of 0.0513. Notably, the model is more advanced than other algorithms. In addition, this paper offers some countermeasures and suggestions to promote healthy building in China. Developing and applying this model can promote and popularize healthy building technology in China and even the globe and contribute to a healthier and more sustainable living environment.
Whether Behavioral Guidance Policies of Construction Waste Resource Utilization Are Effective for Construction Contractors: Evidence from China
Authors: Zhao Na , Liu Qin , Zhang Zhigang , Gao Ke
Published: September 2024

This research endeavors to delve comprehensively into the ramifications of behavioral guidance policies pertaining to construction waste resource utilization (CWRU) on the behavioral awareness and attitudes of Chinese construction contractors. The implicit attitudes of 98 construction contractor workers toward construction waste collection, sorting, and reuse were quantitatively assessed by simulating different types of behavior-guided policies using the External Affective Simon Task (EAST) method with unconscious reaction time as an indicator. At the same time, the questionnaire survey was combined to collect and analyze the scores of these workers’ exogenous attitudes toward CWRU, with a view to revealing the contractors’ intrinsic behavioral awareness and exogenous attitudinal tendencies. The study reveals that economic incentive behavioral guidance policies are pivotal in fostering the collection, sorting, and reuse of construction waste. Furthermore, it novelly uncovers the distinct advantages of laws and regulations frameworks in safeguarding the fundamental aspects of construction waste collection. Notably, publicity and education behavioral policies emerge as a cornerstone in elevating the significance of construction waste collection and sorting, highlighting the cruciality of enhancing public awareness and knowledge to propel CWRU. The results of the study not only provide empirical evidence to understand the inherent attitudes of construction contractor workers toward CWRU behavior but also provide practical support for future policymakers. By advocating for economic incentives, strengthening regulations, and leading education, the multi-pronged approach promotes China’s CWRU accelerates the green transformation of the construction industry.
A Thermal Model for Rural Housing in Mexico: Towards the Construction of an Internal Temperature Assessment System Using Aerial Thermography
Authors: Moctezuma-Sánchez Miguel , Espinoza Gómez David , López-Sosa Luis Bernardo , Golpour Iman , Morales-Máximo Mario , González-Carabes Ricardo
Published: September 2024

Estimating energy flows that affect temperature increases inside houses is crucial for optimizing building design and enhancing the comfort of living spaces. In this study, a thermal model has been developed to estimate the internal temperature of rural houses in Mexico using aerial thermography. The methodology used in this study considered three stages: (a) generating a semi-experimental thermal model of heat transfer through roofs for houses with high infiltration, (b) validating the model using contact thermometers in rural community houses, and (c) integrating the developed model using aerial thermography and Python 3.11.4 into user-friendly software. The results demonstrate that the thermal model is effective, as it was tested on two rural house configurations and achieved an error margin of less than 10% when predicting both maximum and minimum temperatures compared to actual measurements. The model consistently estimates the internal house temperatures using aerial thermography by measuring the roof temperatures. Experimental comparisons of internal temperatures in houses with concrete and asbestos roofs and the model’s projections showed deviations of less than 3 °C. The developed software for this purpose relies solely on the fundamental thermal properties of the roofing materials, along with the maximum roof temperature and ambient temperature, making it both efficient and user-friendly for rural community management systems. Additionally, the model identified areas with comfortable temperatures within different sections of a rural community, demonstrating its effectiveness when integrated with aerial thermography. These findings suggest the potential to estimate comfortable temperature ranges in both rural and urban dwellings, while also encouraging the development of public policies aimed at improving rural housing.
Enhancing Energy Efficiency in Moroccan Construction through Innovative Materials: A Case Study in a Semiarid Climate
Authors: Imghoure Oumaima , Belouaggadia Naoual , Zaite Abdelkabir , Ezzine Mohammed , Lbibb Rachid , Sebaibi Nassim
Published: September 2024

Rising global energy demand has intensified the need for sustainable building practices and reduced energy consumption in the construction sector. This study investigates the energy-saving potential of integrating innovative materials into building wall structures in semiarid climates. Specifically, we examine the combination of thermal insulation made from recycled textile waste and phase change materials (PCMs) in exterior walls. Using the dynamic simulation tool TRNSYS, we analyzed heat transfer through the modified wall assembly under semiarid climate conditions typical of Marrakech, Morocco. Our results show that this “bioclimatic” design significantly impacts cooling loads more than heating demands. The modified building achieved a 52% reduction in summer energy usage compared to a conventional reference building. This energy saving translates to a 39% decrease in greenhouse gas emissions. Importantly, this study confirms that this configuration maintains thermal comfort for occupants, with particular effectiveness during the hot summer months when cooling demands are highest.
Dynamic Compaction of High Groundwater Level Subgrade of Buildings: A Model Test-Based Analysis of Stress Distribution and Reinforcement Mechanisms
Authors: Sun Jingyuan , Li Peixuan , Ge Xinsheng , Wang Jingyue , Liu Yasheng , Tian Shiyu
Published: September 2024

Dynamic compaction (DC) represents a cost-effective method for reinforcing subgrade and is particularly suited to treating large-scale building subgrade. Nevertheless, the effect of DC reinforcement on high groundwater level (HGL) subgrade remains uncertain due to the lack of clarity surrounding the energy transfer mechanism of DC in HGL subgrade. In this paper, an outdoor model test of HGL subgrade was conducted based on the DC method. The temporal evolution of the internal transverse and vertical dynamic stresses in soil under different water levels, energy levels, and tamper weight conditions was monitored, and the DC mechanism of HGL subgrade was described from the perspectives of the dynamic stress waveform, peak development, and attenuation. On this basis, a novel methodology for assessing the extent of subgrade reinforcement through the utilization of impulses was put forth, thereby facilitating a more precise evaluation. The results showed that the HGL is obstructive in DC energy transfer. The peak dynamic stress, depth of impact and maximum impulse per unit area were markedly diminished when tamping the water surface. The study results also recommend that construction could expand the application range of the DC method and provide engineering suggestions for the selection of construction parameters and subsequent building construction.
Proteomic Profiling Identifies Predictive Signatures for Progression Risk in Patients with Advanced-Stage Follicular Lymphoma
Authors: Hemmingsen Jonas Klejs , Enemark Marie Hairing , Sørensen Emma Frasez , Lauridsen Kristina Lystlund , Hamilton-Dutoit Stephen Jacques , Kridel Robert , Honoré Bent , Ludvigsen Maja
Published: September 2024

Background: Follicular lymphoma (FL) is characterized by an indolent nature and generally favorable prognosis, yet poses a particular clinical challenge, since disease progression is observed in a notable subset of patients. Currently, it is not possible to anticipate which patients will be at risk of progression, highlighting the need for reliable predictive biomarkers that can be detected early in the disease. Methods: We applied tandem-mass-tag labelled nano-liquid chromatography tandem mass spectrometry (nLC-MS/MS) on 48 diagnostic formalin-fixed, paraffin-embedded tumor samples from patients with advanced-stage FL. Of these, 17 experienced subsequent progression (subsequently-progressing, sp-FL) while 31 did not (non-progressing, np-FL). Results: We identified 99 proteins that were significantly differentially expressed between sp-FL samples and np-FL samples (p < 0.05; log2-fold changes between 0.2 and −1.3). Based on this subset of proteins, we classified patients into high-risk and low-risk subgroups using unsupervised machine learning techniques. Pathway analyses of the identified proteins revealed aberrancies within the immune system and cellular energy metabolism. In addition, two proteins were selected for immunohistochemical evaluation, namely stimulator of interferon genes 1 (STING1) and isocitrate dehydrogenase 2 (IDH2). Notably, IDH2 retained significantly lower expression levels in sp-FL samples compared with np-FL samples (p = 0.034). Low IDH2 expression correlated with shorter progression-free survival (PFS, p = 0.020). Conclusions: This study provides evidence for some of the biological mechanisms likely to be involved in FL progression and, importantly, identifies potential predictive biomarkers for improvement of risk stratification up-front at time of FL diagnosis.
AAMP and MTSS1 Are Novel Negative Regulators of Endothelial Barrier Function Identified in a Proteomics Screen
Authors: Podieh Fabienne , Overboom Max C. , Knol Jaco C. , Piersma Sander R. , Goeij-de Haas Richard , Pham Thang V. , Jimenez Connie R. , Hordijk Peter L.
Published: September 2024

Cell–cell adhesion in endothelial monolayers is tightly controlled and crucial for vascular integrity. Recently, we reported on the importance of fast protein turnover for maintenance of endothelial barrier function. Specifically, continuous ubiquitination and degradation of the Rho GTPase RhoB is crucial to preserve quiescent endothelial integrity. Here, we sought to identify other barrier regulators, which are characterized by a short half-life, using a proteomics approach. Following short-term inhibition of ubiquitination with E1 ligase inhibitor MLN7243 or Cullin E3 ligase inhibitor MLN4924 in primary human endothelial cells, we identified sixty significantly differentially expressed proteins. Intriguingly, our data showed that AAMP and MTSS1 are novel negative regulators of endothelial barrier function and that their turnover is tightly controlled by ubiquitination. Mechanistically, AAMP regulates the stability and activity of RhoA and RhoB, and colocalizes with F-actin and cortactin at membrane ruffles, possibly regulating F-actin dynamics. Taken together, these findings demonstrate the critical role of protein turnover of specific proteins in the regulation of endothelial barrier function, contributing to our options to target dysregulation of vascular permeability.
The Immune Response in Two Models of Traumatic Injury of the Immature Brain
Authors: Al-Khateeb Zahra F. , Henson Siân M. , Tremoleda Jordi L. , Michael-Titus Adina T.
Published: September 2024

Traumatic brain injury (TBI) can cause major disability and increases the risk of neurodegeneration. Post-TBI, there is infiltration of peripheral myeloid and lymphoid cells; there is limited information on the peripheral immune response post-TBI in the immature brain—where injury may interfere with neurodevelopment. We carried out two injury types in juvenile mice: invasive TBI with a controlled cortical impact (CCI) and repetitive mild TBI (rmTBI) using weight drop injury and analysed the response at 5- and 35-days post-injury. In the two models, we detected the brain infiltration of immune cells (e.g., neutrophils, monocytes, dendritic cells, CD4+ T cells, and NK cells). There were increases in macrophages, neutrophils, and dendritic cells in the spleen, increases in dendritic cells in blood, and increases in CD8+ T cells and B cells in lymph nodes. These results indicate a complex peripheral immune response post-TBI in the immature brain, with differences between an invasive injury and a repetitive mild injury.
A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression
Authors: Park Hyun , Lee Sang Min , Jeong Su Ji , Kweon Yeong Cheon , Shin Go Woon , Kim Whi Young , Lee-Kwon Whaseon , Park Chan Young , Kwon Hyug Moo
Published: September 2024

Human coronaviruses (HCoVs) modify host proteins to evade the antiviral defense and sustain viral expansion. Here, we report tonicity-responsive enhancer (TonE) binding protein (TonEBP) as a cellular target of HCoVs. TonEBP was cleaved into N-terminal and C-terminal fragments (TonEBP NT and TonEBP CT, respectively) by NSP5 from all the HCoVs tested. This cleavage resulted in the loss of TonEBP’s ability to stimulate the TonE-driven transcription. On the other hand, TonEBP NT promoted viral expansion in association with the suppression of IFN-β expression. TonEBP NT competed away NF-κB binding to the PRD II domain on the IFN-β promoter. A TonEBP mutant resistant to the cleavage by NSP5 did not promote the viral expansion nor suppress the IFN-β expression. These results demonstrate that HCoVs use a common strategy of targeting TonEBP to suppress the host immune defense.
Effect of Volume Fraction of Carbon Nanotubes on Structure Formation in Polyacrylonitrile Nascent Fibers: Mesoscale Simulations
Authors: Komarov Pavel , Malyshev Maxim , Baburkin Pavel , Guseva Daria
Published: September 2024

We present a mesoscale model and the simulation results of a system composed of polyacrylonitrile (PAN), carbon nanotubes (CNTs), and a mixed solvent of dimethylsulfoxide (DMSO) and water. The model describes a fragment of a nascent PAN/CNT composite fiber during coagulation. This process represents one of the stages in the production of PAN composite fibers, which are considered as precursors for carbon fibers with improved properties. All calculations are based on dynamic density functional theory. The results obtained show that the greatest structural heterogeneity of the system is observed when water dominates in the composition of the mixed solvent, which is identified with the conditions of a non-solvent coagulation bath. The model also predicts that the introduction of CNTs can lead to an increase in structural heterogeneity in the polymer matrix with increasing water content in the system. In addition, it is shown that the presence of a surface modifier on the CNT surface, which increases the affinity of the filler to the polymer, can sufficiently reduce the inhomogeneity of the nascent fiber structure.
Cyclic Loading of Metallic Glasses Prepared Using Cryogenic Treatments
Authors: Amigo Nicolás
Published: September 2024

This study investigates the degradation of mechanical properties in CuZr metallic glasses (MGs) under cyclic loading using molecular dynamics simulations. Both as-cast (AC) and cryogenically thermal-cycled (TC) samples with Cu36Zr64 and Cu64Zr36 compositions were analyzed. Results show that cyclic loading significantly degrades Young’s modulus, ultimate tensile stress, and toughness, with most pronounced reductions occurring in the initial cycles. TC samples exhibit improved ductility and stability compared to AC samples. In contrast, AC samples demonstrate greater initial strength but faster degradation. Cu-rich samples maintain higher strength but degrade similarly to Cu-poor samples. The AC Cu-rich sample experiences more localized deformation and delayed degradation, while TC and AC Cu-poor samples quickly reach a steady state. These findings highlight the influence of atomic composition and thermal treatments on the mechanical performance and degradation behavior of MGs.
The Effects of Rice Husk Ash as Bio-Cementitious Material in Concrete
Authors: Alsaed Mays Mahmoud , Al Mufti Rafal Latif
Published: September 2024

Concrete is one of the most commonly used materials in civil engineering construction, and it continues to have increased production. This puts pressure on the consumption of its constituent materials, including Portland cement and aggregates. There are environmental consequences related to the increased emission of CO2 that are associated with the production process of Portland cement. This has led to the development and use of alternative cementitious materials, mainly in the form of condensed silica fume, pulverised fuel ash, and ground granulated blast furnace slag. All of these are by-products of the silicon, electrical power generation, and iron production industries, respectively. In recent years, attention has turned to the possible use of sustainable bio-waste materials that might contribute to the replacement of Portland cement in concrete. This research investigates the effects of using rice husk ash as cement replacement material on the 1 to 28-day concrete properties, including the compressive strength, workability, and durability of concrete. The findings indicate that including rice husk ash in concrete can improve its strength at 3–28 days for percentage replacements of 5% to 20% (ranging from 2.4% to 18.7% increase) and improvements from 1 day for 20% replacement (with 11.1% increase). Any percentage replacement with rice husk ash also reduced the air permeability by 21.4% and therefore improved the durability, while there was a small reduction in the workability with increased replacement.