bluuebunny/crossref_metadata_2025 huggingface dataset:
Increasing Temperature Facilitates Polyp Spreading and Medusa Appearance of the Invasive Hydrozoan Craspedacusta sowerbii
Authors: Marchessaux Guillaume , Lüskow Florian , Bejean Mickaël , Pakhomov Evgeny A.
Published: July 2022

The freshwater jellyfish Craspedacusta sowerbii is among the most widespread invasive species, observed across a wide temperature range. The aim of this study is to analyze the polyp and medusa stages response to different temperatures by using (i) an experimental study on the polyp colony growth at 19 and 29 °C, and (ii) prediction of the Thermal Habitat Suitability (THS) based on the thermal tolerance of the medusa stage. The total number of polyps and colonies was greater at high temperature. At 19 °C, colonies with 1 to 5 polyps were present, with colonies of 1 to 3 polyps numerically dominating. At 29 °C, colonies were 80% composed of 1- to 2-polyps. Based on the published medusa pulsation rhythm data, a Thermal Performance Curve (TPC) regression was performed and used to monthly predict the THS for current and future (2050 and 2100) scenarios. The southern hemisphere offered optimal conditions (THS > 0.6) year-round. In the northern hemisphere, the optimum period was predicted to be between June and September. The future THS were considerably larger than at present with an increase in optimal THS at higher latitudes (up to 60° N). The combination of experimental and modeling approaches allows to identify the optimal thermal conditions of the polyp and medusa stages and to predict their invasive capacities.
Corin Deficiency Alters Adipose Tissue Phenotype and Impairs Thermogenesis in Mice
Authors: Zhang Xianrui , Li Wenguo , Zhou Tiantian , Liu Meng , Wu Qingyu , Dong Ningzheng
Published: July 2022

Atrial natriuretic peptide (ANP) is a key regulator in body fluid balance and cardiovascular biology. In addition to its role in enhancing natriuresis and vasodilation, ANP increases lipolysis and thermogenesis in adipose tissue. Corin is a protease responsible for ANP activation. It remains unknown if corin has a role in regulating adipose tissue function. Here, we examined adipose tissue morphology and function in corin knockout (KO) mice. We observed increased weights and cell sizes in white adipose tissue (WAT), decreased levels of uncoupling protein 1 (Ucp1), a brown adipocyte marker in WAT and brown adipose tissue (BAT), and suppressed thermogenic gene expression in BAT from corin KO mice. At regular room temperature, corin KO and wild-type mice had similar metabolic rates. Upon cold exposure at 4 °C, corin KO mice exhibited impaired thermogenic responses and developed hypothermia. In BAT from corin KO mice, the signaling pathway of p38 mitogen-activated protein kinase, peroxisome proliferator-activated receptor c coactivator 1a, and Ucp1 was impaired. In cell culture, ANP treatment increased Ucp1 expression in BAT-derived adipocytes from corin KO mice. These data indicate that corin mediated-ANP activation is an important hormonal mechanism in regulating adipose tissue function and body temperature upon cold exposure in mice.
Science Revealing Ancient Magic: Phytolith Evidence from the Early Chalcolithic Site of Isaiia (Eastern Romania)
Authors: Tencariu Felix Adrian , Delhon Claire , Vornicu Diana Măriuca , Asăndulesei Andrei , Brașoveanu Casandra , Danu Mihaela
Published: July 2022

The article presents the palaeobotanical investigations of a remarkable discovery from the Early Chalcolithic settlement of Isaiia–Balta Popii (Romania), a multi-layered site. The excavation of a dwelling brought to light a rather rare finding, meaning a medium sized ceramic vessel having deposited inside two objects of burnt clay: an anthropomorphic figurine depicting pregnancy attributes and a small cone. Given the special character of the deposition, several samples from the vessel and near it were collected for phytolith analysis. Our results highlighted a ritual plant deposition: Elongate dendritic and Blocky morphotypes suggest that cereals and probably Artemisia seem to have been used for this purpose. These plants are known, aside from their practical uses, as powerful symbols, used through the ages in magic practices. All of these facts are strong arguments to interpret this find as a result of a ritual related to fertility involving both feminine and masculine symbols and plant use.
Application of Micro-Computed Tomography for the Estimation of the Post-Mortem Interval of Human Skeletal Remains
Authors: Schmidt Verena-Maria , Zelger Philipp , Woess Claudia , Pallua Anton K. , Arora Rohit , Degenhart Gerald , Brunner Andrea , Zelger Bettina , Schirmer Michael , Rabl Walter , Pallua Johannes D.
Published: July 2022

It is challenging to estimate the post-mortem interval (PMI) of skeletal remains within a forensic context. As a result of their interactions with the environment, bones undergo several chemical and physical changes after death. So far, multiple methods have been used to follow up on post-mortem changes. There is, however, no definitive way to estimate the PMI of skeletal remains. This research aimed to propose a methodology capable of estimating the PMI using micro-computed tomography measurements of 104 human skeletal remains with PMIs between one day and 2000 years. The present study indicates that micro-computed tomography could be considered an objective and precise method of PMI evaluation in forensic medicine. The measured parameters show a significant difference regarding the PMI for Cort Porosity p < 0.001, BV/TV p > 0.001, Mean1 p > 0.001 and Mean2 p > 0.005. Using a machine learning approach, the neural network showed an accuracy of 99% for distinguishing between samples with a PMI of less than 100 years and archaeological samples.
Integrative Organelle-Based Functional Proteomics: In Silico Prediction of Impaired Functional Annotations in SACS KO Cell Model
Authors: Morani Federica , Doccini Stefano , Galatolo Daniele , Pezzini Francesco , Soliymani Rabah , Simonati Alessandro , Lalowski Maciej M. , Gemignani Federica , Santorelli Filippo M.
Published: July 2022

Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is an inherited neurodegenerative disease characterized by early-onset spasticity in the lower limbs, axonal-demyelinating sensorimotor peripheral neuropathy, and cerebellar ataxia. Our understanding of ARSACS (genetic basis, protein function, and disease mechanisms) remains partial. The integrative use of organelle-based quantitative proteomics and whole-genome analysis proposed in the present study allowed identifying the affected disease-specific pathways, upstream regulators, and biological functions related to ARSACS, which exemplify a rationale for the development of improved early diagnostic strategies and alternative treatment options in this rare condition that currently lacks a cure. Our integrated results strengthen the evidence for disease-specific defects related to bioenergetics and protein quality control systems and reinforce the role of dysregulated cytoskeletal organization in the pathogenesis of ARSACS.
Lipopolysaccharide Exposure Differentially Alters Plasma and Brain Inflammatory Markers in Adult Male and Female Rats
Authors: Nonoguchi Hannah A. , Kouo Timothy Wee Shang , Kortagere Sandhya , Hillman Joshua , Boyle David L. , Mandyam Chitra D.
Published: July 2022

Humans and rodents have sexually dimorphic immune responses, which could influence the brain’s response to a systemic inflammatory insult. Lipopolysaccharide (LPS) is a stimulator of the innate immune system and is routinely used in animal models to study blood–brain barrier (BBB) dysfunction under inflammatory conditions. Therefore, we examined whether inflammatory response to LPS and the associated BBB disruption differed in male and female adult rats. Rats were treated with saline or two injections of 1 mg/kg LPS and studied 24 h after the second LPS injection. Plasma isolated from trunk blood and brain tissue homogenates of the prefrontal cortex (PFC), dorsal striatum (DS), hippocampus, and cerebellum were analyzed for cytokines and chemokines using a 9-plex panel from Meso Scale Discovery. BBB disruption was analyzed with tight junction proteins claudin-5 and VE-cadherin via Western blotting and VEGF by ELISA. This allowed us to compare sex differences in the levels of individual cytokines as well as associations among cytokines and expression of tight junction proteins between the plasma and specific brain regions. Higher levels of interferon-γ, interleukin-10 (IL-10), IL-13, IL-4, CXCL-1, and VEGF in the plasma were revealed compared to the brain homogenates, and higher levels of TNFα, IL-1β, IL-6, and IL-5 in the PFC were seen compared with plasma and other brain regions in males. Females showed higher levels of plasma CXCL1 and VEGF compared to males, and males showed higher levels of PFC TNFα, IL-6, IL-4, and VEGF compared to females. LPS induced significant increases in plasma cytokines and VEGF in both sexes. LPS did not significantly alter cytokines in brain tissue homogenates, however, it increased chemokines in the PFC, DS, and hippocampus. In the PFC, LPS produced BBB disruption, which is evident as reduced expression of claudin-5 in males and reduced expression of VE-cadherin in both sexes. Taken together, our results reveal significant sex differences in pro-inflammatory cytokine and chemokine levels in plasma and brain that were associated with BBB disruption after LPS, and validate the use of multiplex assay for plasma and brain tissue samples.
Investigating the Relationship between Facial Mimicry and Empathy
Authors: Kovalchuk Yevgeniya , Budini Elizabeta , Cook Robert M. , Walsh Andrew
Published: July 2022

Facial expressions play a key role in interpersonal communication when it comes to negotiating our emotions and intentions, as well as interpreting those of others. Research has shown that we can connect to other people better when we exhibit signs of empathy and facial mimicry. However, the relationship between empathy and facial mimicry is still debated. Among the factors contributing to the difference in results across existing studies is the use of different instruments for measuring both empathy and facial mimicry, as well as often ignoring the differences across various demographic groups. This study first looks at the differences in the empathetic abilities of people across different demographic groups based on gender, ethnicity and age. The empathetic ability is measured based on the Empathy Quotient, capturing a balanced representation of both emotional and cognitive empathy. Using statistical and machine learning methods, this study then investigates the correlation between the empathetic ability and facial mimicry of subjects in response to images portraying different emotions displayed on a computer screen. Unlike the existing studies measuring facial mimicry using electromyography, this study employs a technology detecting facial expressions based on video capture and deep learning. This choice was made in the context of increased online communication during and after the COVID-19 pandemic. The results of this study confirm the previously reported difference in the empathetic ability between females and males. However, no significant difference in empathetic ability was found across different age and ethnic groups. Furthermore, no strong correlation was found between empathy and facial reactions to faces portraying different emotions shown on a computer screen. Overall, the results of this study can be used to inform the design of online communication technologies and tools for training empathy team leaders, educators, social and healthcare providers.
Boredom Proneness and Rule-Breaking: A Persistent Relation One Year into the COVID-19 Pandemic
Authors: Drody Allison C. , Hicks Lydia J. , Danckert James
Published: July 2022

Research conducted within the first year of the pandemic demonstrated that boredom prone individuals were more likely to break rules (e.g., social distancing) aimed at preventing the spread of COVID-19. It is of interest whether this relation persisted deeper into the pandemic, given that initial results may have reflected the extraordinary nature of the early stages of the pandemic on one hand, or more stable dispositions on the other. Therefore, in the Summer of 2021, we administered an online survey to investigate whether boredom proneness predicted COVID-19 rule-breaking over one year into the pandemic (and approximately one year after the earlier studies). We found that boredom prone individuals remained more likely to engage in COVID-19 rule-breaking. Our results suggest that a trait disposition towards boredom exerts a persistent, long-term influence on behaviour, one that is detrimental to personal well-being during the pandemic. Adherence to public health measures might be improved by encouraging individuals to find adaptive ways of coping with boredom.
Emerging Roles of Chemokines in Cancer Immunotherapy
Authors: Moser Bernhard
Published: July 2022

This series of 15 articles (4 original articles and 11 reviews) is presented by international leaders in chemokine research [...]
Photochemical Internalization of siRNA for Cancer Therapy
Authors: Ali Lamiaa Mohamed Ahmed , Gary-Bobo Magali
Published: July 2022

In the race to design ever more effective therapy with ever more focused and controlled actions, nanomedicine and phototherapy seem to be two allies of choice. Indeed, the use of nanovectors making it possible to transport and protect genetic material is becoming increasingly important. In addition, the use of a method allowing the release of genetic material in a controlled way in space and time is also a strategy increasingly studied thanks to the use of lasers. In parallel, the use of interfering RNA and, more particularly, of small-interfering RNA (siRNA) has demonstrated significant potential for gene therapy. In this review, we focused on the design of the different nanovectors capable of transporting siRNAs and releasing them so that they can turn off the expression of deregulated genes in cancers through controlled photoexcitation with high precision. This mechanism, called photochemical internalization (PCI), corresponds to the lysosomal leakage of the cargo (siRNA in this case) after destabilization of the lysosomal membrane under light excitation.
Interactions between COVID-19 and Lung Cancer: Lessons Learned during the Pandemic
Authors: Bian David J. H. , Sabri Siham , Abdulkarim Bassam S.
Published: July 2022

Cancer patients, specifically lung cancer patients, show heightened vulnerability to severe COVID-19 outcomes. The immunological and inflammatory pathophysiological similarities between lung cancer and COVID-19-related ARDS might explain the predisposition of cancer patients to severe COVID-19, while multiple risk factors in lung cancer patients have been associated with worse COVID-19 outcomes, including smoking status, older age, etc. Recent cancer treatments have also been urgently evaluated during the pandemic as potential risk factors for severe COVID-19, with conflicting findings regarding systemic chemotherapy and radiation therapy, while other therapies were not associated with altered outcomes. Given this vulnerability of lung cancer patients for severe COVID-19, the delivery of cancer care was significantly modified during the pandemic to both proceed with cancer care and minimize SARS-CoV-2 infection risk. However, COVID-19-related delays and patients’ aversion to clinical settings have led to increased diagnosis of more advanced tumors, with an expected increase in cancer mortality. Waning immunity and vaccine breakthroughs related to novel variants of concern threaten to further impede the delivery of cancer services. Cancer patients have a high risk of severe COVID-19, despite being fully vaccinated. Numerous treatments for early COVID-19 have been developed to prevent disease progression and are crucial for infected cancer patients to minimize severe COVID-19 outcomes and resume cancer care. In this literature review, we will explore the lessons learned during the COVID-19 pandemic to specifically mitigate COVID-19 treatment decisions and the clinical management of lung cancer patients.
Comparison between Endoscopic Submucosal Dissection and Surgery in Patients with Early Gastric Cancer
Authors: Qian Meng , Sheng Yuan , Wu Min , Wang Song , Zhang Kaiguang
Published: July 2022

Background: Endoscopic submucosal dissection (ESD) has become a preferred treatment option for early gastric cancer (EGC). This study aimed to compare the clinical outcomes of ESD and surgical resection for EGC. Methods: This was a retrospective case–control study. Patients with a diagnosis of EGC who underwent ESD or surgery in our hospital from 2011 to 2020 were enrolled. We compared the clinical characteristics and treatment outcomes of these two groups according to propensity score-matching. The primary outcome comparison was overall survival (OS). Secondary outcomes were disease-specific survival (DSS), recurrence-free survival (RFS), and treatment-related events. Results: In the matched cohort, the ESD group showed comparable OS, RFS, and DSS with the surgery group. Statistical differences were shown in blood loss and adverse events. Furthermore, the ESD group showed lower hospital cost, as well as a shorter operative time and hospital duration than the surgery group. The R0 resection and recurrence rates were similar between the two groups. In Cox regression analysis, age, tumor size, poor differentiation, and lymphovascular invasion were regarded as independent factors of OS. Conclusions: With sufficient safety and advantages, ESD can be a first-line treatment of EGC. Preoperative evaluation is vital to the appropriate treatment and prognosis.
A Negative Regulatory Role for RKIP in Breast Cancer Immune Response
Authors: Bach Vu N. , Ding Jane , Yeung Miranda , Conrad Taylor , Odeh Hussain N. , Cubberly Paige , Figy Christopher , Ding Han-Fei , Trumbly Robert , Yeung Kam C.
Published: July 2022

Raf-1 kinase inhibitor protein was first identified as a negative regulator of the Raf signaling pathway. Subsequently, it was shown to have a causal role in containing cancer progression and metastasis. Early studies suggested that RKIP blocks cancer progression by inhibiting the Raf-1 pathway. However, it is not clear if the RKIP tumor and metastasis suppression function involve other targets. In addition to the Raf signaling pathway, RKIP has been found to modulate several other signaling pathways, affecting diverse biological functions including immune response. Recent advances in medicine have identified both positive and negative roles of immune response in cancer initiation, progression and metastasis. It is possible that one way that RKIP exerts its effect on cancer is by targeting an immune response mechanism. Here, we provide evidence supporting the causal role of tumor and metastasis suppressor RKIP in downregulating signaling pathways involved with immune response in breast cancer cells and discuss its potential ramification on cancer therapy.
Satisfaction with Long-Term Aesthetic and 10 Years Oncologic Outcome following Risk-Reducing Mastectomy and Implant-Based Breast Reconstruction with or without Nipple Preservation
Authors: O’Connell Rachel Louise , Tasoulis Marios Konstantinos , Hristova Evguenia , Teoh Victoria , Agusti Ana , Ward Ann , Montgomery Catherine , Mohammed Kabir , Self Janet , Rusby Jennifer E. , Gui Gerald
Published: July 2022

Incidence of bilateral risk-reducing mastectomies (RRMs) is increasing. The aim of this study was to compare satisfaction, aesthetic and oncological outcomes in women undergoing RRM with implant-based reconstruction comparing nipple-sparing mastectomy (NSM) with skin-sparing mastectomy (SSM) (sacrificing the nipple +/− nipple reconstruction). Women who had undergone bilateral RRM between 1997 and 2016 were invited. Aesthetic outcome and nipple symmetry were evaluated using standardized anthropometric measurements. The oncological outcome was assessed at last documented follow up. Ninety-three women (186 breasts) participated, 60 (64.5%) had NSM, 33 (35.5%) SSM. Median time between surgery and participation was 98.4 months (IQR: 61.7–133.9). Of the women, 23/33 (69.7%) who had SSM underwent nipple reconstruction. Nipple projection was shorter in the reconstructed SSM group than the maintained NSM group (p < 0.001). There was no significant difference in overall symmetry (p = 0.670), satisfaction regarding nipple preservation (p = 0.257) or overall nipple satisfaction (p = 0.074). There were no diagnoses of breast cancer at a median follow up of 129 months (IQR: 65–160.6). Women who undergo nipple-sparing RRM maintain long-term nipple symmetry. Nipple projection was less maintained after nipple reconstruction. Although satisfaction with the nipples was higher in the NSM group, this did not reach statistical significance. No breast cancers developed after RRM with long-term follow up.
Comparative Analysis for the Distinction of Chromophobe Renal Cell Carcinoma from Renal Oncocytoma in Computed Tomography Imaging Using Machine Learning Radiomics Analysis
Authors: Alhussaini Abeer J. , Steele J. Douglas , Nabi Ghulam
Published: July 2022

Background: ChRCC and RO are two types of rarely occurring renal tumors that are difficult to distinguish from one another based on morphological features alone. They differ in prognosis, with ChRCC capable of progressing and metastasizing, but RO is benign. This means discrimination of the two tumors is of crucial importance. Objectives: The purpose of this research was to develop and comprehensively evaluate predictive models that can discriminate between ChRCC and RO tumors using Computed Tomography (CT) scans and ML-Radiomics texture analysis methods. Methods: Data were obtained from 78 pathologically confirmed renal masses, scanned at two institutions. Data from the two institutions were combined to form a third set resulting in three data cohorts, i.e., cohort 1, 2 and combined. Contrast-enhanced scans were used and the axial cross-sectional slices of each tumor were extracted from the 3D data using a semi-automatic segmentation technique for both 2D and 3D scans. Radiomics features were extracted before and after applying filters and the dimensions of the radiomic features reduced using the least absolute shrinkage and selection operator (LASSO) method. Synthetic minority oversampling technique (SMOTE) was applied to avoid class imbalance. Five ML algorithms were used to train models for predictive classification and evaluated using 5-fold cross-validation. Results: The number of selected features with good model performance was 20, 40 and 6 for cohorts 1, 2 and combined, respectively. The best model performance in cohorts 1, 2 and combined had an excellent Area Under the Curve (AUC) of 1.00 ± 0.000, 1.00 ± 0.000 and 0.87 ± 0.073, respectively. Conclusions: ML-based radiomics signatures are potentially useful for distinguishing ChRCC and RO tumors, with a reliable level of performance for both 2D and 3D scanning.
Adsorption of Methylene Blue on Chestnut Shell-Based Activated Carbon: Calculation of Thermodynamic Parameters for Solid–Liquid Interface Adsorption
Authors: Kong Lingjian , Zhang Mingyang
Published: July 2022

Chestnut shell-based activated carbon was prepared with chestnut shell as the raw material and ZnCl2 as the activating agent. Based on thermodynamic parameters, the adsorption behavior of methylene blue (MB) on chestnut shell-based activated carbon was studied, and the effect of temperature on the thermodynamic parameters and adsorption behavior was investigated. The Langmuir equilibrium constant (KL) and the standard equilibrium constant (K0) were used to calculate the thermodynamic parameters, respectively. Comparative analysis showed that spontaneous adsorption (ΔG0 < 0) was more compatible with the standard equilibrium constant (K0). Furthermore, the thermodynamic parameters at 30, 40 and 50 °C were measured, and the adsorption potential was investigated and calculated. Eventually, the mechanism of the adsorption process was determined. It was concluded that the adsorption process mainly involved chemical adsorption, which indicated that MB adsorption was caused by the force of the chemical bond.
Canadian Contributions in Fibroblast Biology
Authors: Al-Hattab Danah S. , Chattopadhyaya Sikta , Czubryt Michael P.
Published: July 2022

Fibroblasts are stromal cells found in virtually every tissue and organ of the body. For many years, these cells were often considered to be secondary in functional importance to parenchymal cells. Over the past 2 decades, focused research into the roles of fibroblasts has revealed important roles for these cells in the homeostasis of healthy tissue, and has demonstrated that activation of fibroblasts to myofibroblasts is a key step in disease initiation and progression in many tissues, with fibrosis now recognized as not only an outcome of disease, but also a central contributor to tissue dysfunction, particularly in the heart and lungs. With a growing understanding of both fibroblast and myofibroblast heterogeneity, and the deciphering of the humoral and mechanical cues that impact the phenotype of these cells, fibroblast biology is rapidly becoming a major focus in biomedical research. In this review, we provide an overview of fibroblast and myofibroblast biology, particularly in the heart, and including a discussion of pathophysiological processes such as fibrosis and scarring. We then discuss the central role of Canadian researchers in moving this field forwards, particularly in cardiac fibrosis, and highlight some of the major contributions of these individuals to our understanding of fibroblast and myofibroblast biology in health and disease.
Synovial Fluid-Derived Extracellular Vesicles of Patients with Arthritides Contribute to Hippocampal Synaptic Dysfunctions and Increase with Mood Disorders Severity in Humans
Authors: Cambria Clara , Ingegnoli Francesca , Borzi Eleonora , Cantone Laura , Coletto Lavinia Agra , Rizzuto Alessandra Stefania , De Lucia Orazio , Briguglio Sabrina , Ruscica Massimiliano , Caporali Roberto , Bollati Valentina , Buoli Massimiliano , Antonucci Flavia
Published: July 2022

Arthritides are a highly heterogeneous group of disorders that include two major clinical entities, localized joint disorders such as osteoarthritis (OA) and systemic autoimmune-driven diseases such as rheumatoid arthritis (RA). Arthritides are characterized by chronic debilitating musculoskeletal conditions and systemic chronic inflammation. Poor mental health is also one of the most common comorbidities of arthritides. Depressive symptoms which are most prevalent, negatively impact patient global assessment diminishing the probability of achieving the target of clinical remission. Here, we investigated new insights into mechanisms that link different joint disorders to poor mental health, and to this issue, we explored the action of the synovial fluid-derived extracellular vesicles (EVs) on neuronal function. Our data show that the exposure of neurons to different concentrations of EVs derived from both RA and OA synovial fluids (RA-EVs and OA-EVs) leads to increased excitatory synaptic transmission but acts on specific modifications on excitatory or inhibitory synapses, as evidenced by electrophysiological and confocal experiments carried out in hippocampal cultures. The treatment of neurons with EVs membrane is also responsible for generating similar effects to those found with intact EVs suggesting that changes in neuronal ability arise upon EVs membrane molecules′ interactions with neurons. In humans with arthritides, we found that nearly half of patients (37.5%) showed clinically significant psychiatric symptoms (CGIs score ≥ 3), and at least mild anxiety (HAM-A ≥ 7) or depression (MADRS and HAM-D ≥ 7); interestingly, these individuals revealed an increased concentration of synovial EVs. In conclusion, our data showing opposite changes at the excitatory and inhibitory levels in neurons treated with OA- and RA-EVs, lay the scientific basis for personalized medicine in OA and RA patients, and identify EVs as new potential actionable biomarkers in patients with OA/RA with poor mental health.
Antioxidant Systems, lncRNAs, and Tunneling Nanotubes in Cell Death Rescue from Cigarette Smoke Exposure
Authors: Ferrer Jose Lorenzo M. , Garcia Reynaldo L.
Published: July 2022

Cigarette smoke is a rich source of carcinogens and reactive oxygen species (ROS) that can damage macromolecules including DNA. Repair systems can restore DNA integrity. Depending on the duration or intensity of stress signals, cells may utilize various survival and adaptive mechanisms. ROS levels are kept in check through redundant detoxification processes controlled largely by antioxidant systems. This review covers and expands on the mechanisms available to cigarette smoke-exposed cancer cells for restoring the redox balance. These include multiple layers of transcriptional control, each of which is posited to be activated upon reaching a particular stress threshold, among them the NRF2 pathway, the AP-1 and NF-kB pathways, and, finally, TP53, which triggers apoptosis if extreme toxicity is reached. The review also discusses long noncoding RNAs, which have been implicated recently in regulating oxidative stress—with roles in ROS detoxification, the inflammatory response, oxidative stress-induced apoptosis, and mitochondrial oxidative phosphorylation. Lastly, the emerging roles of tunneling nanotubes in providing additional mechanisms for metabolic rescue and the regulation of redox imbalance are considered, further highlighting the expanded redox reset arsenal available to cells.
Regulation of Life & Death by REGγ
Authors: Funderburk Keaton E. , Kang Jungseog , Li Henry J.
Published: July 2022

REGγ, a proteasome activator belonging to the 11S (otherwise known as REG, PA28, or PSME) proteasome activator family, is widely present in many eukaryotes. By binding to the 20S catalytic core particle, REGγ acts as a molecular sieve to selectively target proteins for degradation in an ATP- and ubiquitin-independent manner. This non-canonical proteasome pathway directly regulates seemingly unrelated cellular processes including cell growth and proliferation, apoptosis, DNA damage response, immune response, and metabolism. By affecting different pathways, REGγ plays a vital role in the regulation of cellular life and death through the maintenance of protein homeostasis. As a promoter of cellular growth and a key regulator of several tumor suppressors, many recent studies have linked REGγ overexpression with tumor formation and suggested the REGγ-proteasome as a potential target of new cancer-drug development. This review will present an overview of the major functions of REGγ as it relates to the regulation of cellular life and death, along with new mechanistic insights into the regulation of REGγ.
CYP1A1, VEGFA and Adipokine Responses of Human Adipocytes Co-exposed to PCB126 and Hypoxia
Authors: Amine Zeinab El , Mauger Jean-François , Imbeault Pascal
Published: July 2022

It is increasingly recognized that hypoxia may develop in adipose tissue as its mass expands. Adipose tissue is also the main reservoir of lipophilic pollutants, including polychlorinated biphenyls (PCBs). Both hypoxia and PCBs have been shown to alter adipose tissue functions. The signaling pathways induced by hypoxia and pollutants may crosstalk, as they share a common transcription factor: aryl hydrocarbon receptor nuclear translocator (ARNT). Whether hypoxia and PCBs crosstalk and affect adipokine secretion in human adipocytes remains to be explored. Using primary human adipocytes acutely co-exposed to different levels of hypoxia (24 h) and PCB126 (48 h), we observed that hypoxia significantly inhibits the PCB126 induction of cytochrome P450 (CYP1A1) transcription in a dose-response manner, and that Acriflavine (ACF)—an HIF1α inhibitor—partially restores the PCB126 induction of CYP1A1 under hypoxia. On the other hand, exposure to PCB126 did not affect the transcription of the vascular endothelial growth factor-A (VEGFA) under hypoxia. Exposure to hypoxia increased leptin and interleukin-6 (IL-6), and decreased adiponectin levels dose-dependently, while PCB126 increased IL-6 and IL-8 secretion in a dose-dependent manner. Co-exposure to PCB126 and hypoxia did not alter the adipokine secretion pattern observed under hypoxia and PCB126 exposure alone. In conclusion, our results indicate that (1) hypoxia inhibits PCB126-induced CYP1A1 expression at least partly through ARNT-dependent means, suggesting that hypoxia could affect PCB metabolism and toxicity in adipose tissue, and (2) hypoxia and PCB126 affect leptin, adiponectin, IL-6 and IL-8 secretion differently, with no apparent crosstalk between the two factors.
The Effects of Trauma Types at Pre-Migration, Transit, and Post-Migration Stages on Depression and PTSD among North Korean Refugees in South Korea
Authors: Lee Jin Hyuk
Published: July 2022

This study aimed to assess the interrelationship (comorbidity pattern) between depression and PTSD among North Korean (NK) refugees in South Korea and examine the effects of trauma types at pre-migration, transit, and post-migration stages on both depression and PTSD. Therefore, the study analyzed the data of the 2017 Survey on the Human Right Abuses and Trauma of NK refugees (N = 299) using the Seemingly Unrelated Regression model. The results showed that depression and PTSD have a comorbidity pattern among NK refugees. In addition, the predominant death threat of trauma type at the transit stage had a positive relationship with depression and PTSD simultaneously. Based on these findings, we suggested a transdiagnostic approach and trauma-informed care for NK refugees who suffered from various traumas in the migration stage.
Digital Media Influence on Adolescents’ Behavior during the COVID-19 Pandemic: Self-Intravenous Injection of Lactobacilli Drinking Yogurt
Authors: Koh Eunjung , Choi Insu , Choi Seul-A , Kang Yeo-Jin , Cho Hwa-Jin
Published: July 2022

During the coronavirus disease 2019 (COVID-19) pandemic, widened physical distance and social deprivation are being replaced with digital media use. The media’s social function has tremendously increased following the COVID-19 era and adolescents living in a media-saturated world are the most vulnerable subjects of all. Social media use can encourage risky behavior in adolescents. Posting videos related to risk behaviors on social media has been linked to jeopardizing not only mental health but also physical safety. Herein, we report a case of a 12-year-old boy who intravenously self-injected Lactobacilli drinking yogurt for the purpose of filming a video to post on social media. He was treated with antibiotics based on Lactobacilli sepsis that cured him without any complications. In order to reduce the risk-taking behavior of adolescents, social norms need to be straightened up, and the social responsibility of hosts is strongly recommended.
A Simplified Model for Estimating Household Air Pollution in Challenging Contexts: A Case Study from Ghana
Authors: Vinti Giovanni , Vaccari Mentore
Published: July 2022

Almost three billion people rely primarily on inefficient and polluting cooking systems worldwide. Household air pollution is a direct consequence of this practice, and it is annually associated with millions of premature deaths and diseases, mainly in low- and lower-middle-income countries. The use of improved cookstoves often represents an appropriate solution to reduce such health risks. However, in the distribution of such units, it can be necessary to prioritize the beneficiaries. Thus, in this study, we conducted field research involving five rural villages in the Northern part of Ghana, where using three-stone fires or rural stoves was common. Concentrations of PM2.5, PM10, and carbon monoxide (CO) were measured indoors and outdoors. Considering each field mission lasted less than 24 h, assumptions were made so as to calculate the average pollutant concentrations in 24 h through a new, simplified equation that combined efficiency and cost-savings by shortening field assessments. The obtained values were compared with international guidelines. The results showed that PM2.5 and PM10 limits were overstepped in two villages, which should thus be prioritized. However, further research will be necessary to strengthen and validate our proposed equation, which must be seen as a starting point.
Corrosion Behavior and Sacrificial Properties of Zn and Zn-Al Coatings in Conditions Simulating Deep Geological Disposal of Radioactive Waste at 80 °C
Authors: Martin Alice , Texier-Mandoki Nathalie , Crusset Didier , Sabot René , Creus Juan , Refait Philippe
Published: July 2022

In France, it is planned to manage high-level radioactive waste via deep geological disposal. The carbon steel overpacks containing the waste will face corrosion processes specific to the unusual environment of the disposal. To protect these overpacks against corrosion, the use of metallic sacrificial coatings was considered as a possible solution. Therefore, the corrosion behavior of thermally sprayed Zn and Zn-15wt.%Al coatings (500 µm thick) was studied in a solution simulating the specific environment of the disposal. Galvanic corrosion experiments were performed with a coated steel electrode connected to a bare steel one, which simulated a coating defect. First, it was observed that the Zn coating had lost its sacrificial properties after ~2 months of coupling. XRD and µ-Raman analysis carried out after the experiment demonstrated that the coating was covered with a protective hemimorphite (Zn4Si2O7(OH)2·H2O)-rich layer. In contrast, the Zn-15wt.%Al coating kept its sacrificial properties all along the 6-month experiment. It proved to be covered with a layer mainly composed of Zn and Zn-Al hydroxysulfates, which did not contain hemimorphite. However, SEM cross-sectional observations showed that the electrolyte had seeped into the coating and had even reached the steel surface. This infiltration led to the oxidation of the splats inside the coating, even close to the steel surface, and induced a large swelling of the coating. Its thickness was increased by 50%.
Effect of the Cooling Rate of Thermal Simulation on the Microstructure and Mechanical Properties of Low-Carbon Bainite Steel by Laser-Arc Hybrid Welding
Authors: Chen Jintao , Zhang Zhenlin , Zhang Zhiyi , Liu Yingzong , Zhao Xu , Chen Jingqing , Chen Hui
Published: July 2022

A new kind of low-carbon bainite steel with excellent strength and toughness was developed, serving as the bogie of the next-generation high-speed train. However, the softening of the heat-affected zone (HAZ) in laser-arc hybrid welding (LAHW) needs to be overcome. In this study, the effect of the cooling rate of the LAHW process on the microstructure and mechanical properties in the HAZ was explored via thermal simulation. The results showed that with increased cooling rate, the grain size increased, the content of lath martensite decreased, and the lath bainite gradually changed to a granular shape in the thermal simulation specimen. With the decrease in the cooling rate, i.e., with the increase of t8/5, the strength–toughness matching of the material showed a downward trend. The thermal simulation specimen with a t8/5 of 6~8 s had higher strength and good toughness, which can be considered a potential welding parameter reference. The content of martensitic austenite (M-A) constituents was the main factor that determined the strength and toughness of the joint. During the tensile test, the axial force caused the material to tighten, and the transverse stress as obvious in the part of the M-A constituents that are prone to microcracks and many defects, resulting in cracks, paths, and multi-component layers in the center. As a result, the thermal cycle specimens had mixed fracture characteristics.
COVID-19 Vaccine Acceptance: A Case Study from Nepal
Authors: Gaire Amrit , Panthee Bimala , Basyal Deepak , Paudel Atmika , Panthee Suresh
Published: July 2022

While vaccine acceptance changes over time, and factors determining vaccine acceptance differ depending on disease and region, limited studies have evaluated vaccine acceptance in Nepal. We conducted an online, cross-sectional study to assess COVID-19 vaccine acceptance among Nepalese. Data were collected before and after the vaccine approval in Nepal, during which 576 and 241 responses were obtained, respectively. We found that vaccine acceptance was generally high among Nepalese (93%) and increased after the safety of vaccine was examined by the regulatory bodies (98%). This indicated the role of the national drug regulatory authority to convey drug safety. In addition, we analyzed the predictor(s) of vaccine acceptance. We found that the people who believe that vaccine is an effective measure in preventing and controlling the disease were highly likely to accept vaccination. Given that Nepal had just passed the most devastating wave of COVID-19 during our post-approval data collection, we assume that this might have also played a role in the belief that vaccination is an appropriate approach to combat the pandemic. Likewise, the number of people willing to vaccinate as soon as possible increased from 43% to 86% after approval. Therefore, our results indicate that the government needs to focus on assuring the safety and effectiveness of a vaccine to enhance acceptance. Although fewer responses obtained after vaccine approval might have affected our results, overall, our findings indicate vaccine acceptance is likely to be affected by socio-demographic factors and the attitudes of respondents. This should be carefully considered in the rollout of the vaccination plans in Nepal and countries alike in future.
High-Pressure Solidification of Ternary Al-Ni-Sn Alloy
Authors: Yang Xiaohong , Lv Feng , Wang Xiaohong , Zhang Zhengzhong , Zhu Dongdong , Chen Yuan , Ge Jianya , Tang Jinhua
Published: July 2022

The microstructure, phase composition and mechanical properties of ternary Al-5.4Ni-2Sn (mass fraction) alloy solidified under different high pressures were researched. The results show that the phase composition of the alloy solidified at different pressures is Al, Al3Ni and β-Sn. The thermodynamic phase diagram of the ternary alloy Al-5.4Ni-2Sn was calculated under the equilibrium condition. The results demonstrate that the solidification process is as follows: L→Al3Ni→(α-Al + Al3Ni)eutectic→(α-Al + Al3Ni + β-Sn)eutectic. The hardness values of α-Al phase at ambient pressure, 2 GPa and 4 GPa are 1.5 GPa, 1.62 GPa and 1.99 GPa, respectively. This is an increase of 8% and 32.7%, respectively. The hardness of β-Sn phase decreases by about 31.2% at 4 GPa. When the deformation is 30%, the compressive strength at ambient pressure, 2 GPa and 4 GPa is 538.1 MPa, 1403.2 MPa and 1547.9 MPa, respectively. The compressive strength under high pressure increased by 160.85% and 187.7%, respectively.
Motility Suppression and Trapping Bacteria by ZnO Nanostructures
Authors: Yan Ningzhe , Luo Hao , Liu Yanan , Yu Haiping , Jing Guangyin
Published: July 2022

Regulating the swimming motility of bacteria near surfaces is essential to suppress or avoid bacterial contamination and infection in catheters and medical devices with wall surfaces. However, the motility of bacteria near walls strongly depends on the combination of the local physicochemical properties of the surfaces. To unravel how nanostructures and their local chemical microenvironment dynamically affect the bacterial motility near surfaces, here, we directly visualize the bacterial swimming and systematically analyze the motility of Escherichia coli swimming on ZnO nanoparticle films and nanowire arrays with further ultraviolet irradiation. The results show that the ZnO nanowire arrays reduce the swimming motility, thus significantly enhancing the trapping ability for motile bacteria. Additionally, thanks to the wide bandgap nature of a ZnO semiconductor, the ultraviolet irradiation rapidly reduces the bacteria locomotion due to the hydroxyl and singlet oxygen produced by the photodynamic effects of ZnO nanowire arrays in an aqueous solution. The findings quantitatively reveal how the combination of geometrical nanostructured surfaces and local tuning of the steric microenvironment are able to regulate the motility of swimming bacteria and suggest the efficient inhibition of bacterial translocation and infection by nanostructured coatings.
Distinguishing Long-Discussed Cryptic Species of the Epibiotic Goose-Neck Barnacle of the Genus Conchoderma (Thoracicalcarea: Lepadidae) with Integrative Taxonomy
Authors: Chan Benny K. K. , Chen Yu-Hsuan
Published: July 2022

Naked goose neck barnacles Conchoderma can grow on a wide variety of marine organisms. The taxonomic status of two of its species—C. virgatum and C. hunteri—are currently controversial. Some studies suggest that C. hunteri is a subspecies, variety or growth forms of C. virgatum, because both have great morphological variations, but other studies consider C. hunteri and C. virgatum to be distinct species. The present study examines the morphology and sequence divergence of the COI gene in C. virgatum, C. hunteri and other closely related species. There are consistent morphological differences between C. virgatum and C. hunteri in the tergum, carina and fifth teeth of the mandible. Phylogenetic analysis based on the divergence in the COI gene revealed that C. virgatum and C. hunteri form sister clades with high bootstrap values. The K2P distances within C. hunteri and C. virgatum are 0.034 ± 0.008 and 0.002 ± 0.001 for the COI sequences, respectively. The K2P distance between C. hunteri and C. virgatum is 0.097 ± 0.016. Morphological and molecular evidence confirm that C. hunteri is a valid species.
Isolated Convolutional-Neural-Network-Based Deep-Feature Extraction for Brain Tumor Classification Using Shallow Classifier
Authors: Almalki Yassir Edrees , Ali Muhammad Umair , Kallu Karam Dad , Masud Manzar , Zafar Amad , Alduraibi Sharifa Khalid , Irfan Muhammad , Basha Mohammad Abd Alkhalik , Alshamrani Hassan A. , Alduraibi Alaa Khalid , Aboualkheir Mervat
Published: July 2022

In today’s world, a brain tumor is one of the most serious diseases. If it is detected at an advanced stage, it might lead to a very limited survival rate. Therefore, brain tumor classification is crucial for appropriate therapeutic planning to improve patient life quality. This research investigates a deep-feature-trained brain tumor detection and differentiation model using classical/linear machine learning classifiers (MLCs). In this study, transfer learning is used to obtain deep brain magnetic resonance imaging (MRI) scan features from a constructed convolutional neural network (CNN). First, multiple layers (19, 22, and 25) of isolated CNNs are constructed and trained to evaluate the performance. The developed CNN models are then utilized for training the multiple MLCs by extracting deep features via transfer learning. The available brain MRI datasets are employed to validate the proposed approach. The deep features of pre-trained models are also extracted to evaluate and compare their performance with the proposed approach. The proposed CNN deep-feature-trained support vector machine model yielded higher accuracy than other commonly used pre-trained deep-feature MLC training models. The presented approach detects and distinguishes brain tumors with 98% accuracy. It also has a good classification rate (97.2%) for an unknown dataset not used to train the model. Following extensive testing and analysis, the suggested technique might be helpful in assisting doctors in diagnosing brain tumors.