bluuebunny/crossref_metadata_2025 huggingface dataset:
Family history of PCOS, obesity, low fiber diet, and low physical activity increase the risk of PCOS
Authors: Wahyuni Alfaina , Supriyatiningsih  , Kusumawati Wiwik , Sedah Kirana Kamila , Mayayustika Cynthia Dwi
Published: July 2023

Background: Polycystic ovarian syndrome (PCOS) is a common endocrine disorder and leading cause of prolonged anovulation. PCOS has been linked to a variety of long-term health problems, including: heart disease; metabolic syndrome; and diabetes. It is interesting to know the risk factors for PCOS in local settings.Objective: The aim of this research is to identify PCOS risk factors in our own settings (Asri Medical Center in Yogyakarta), so that we could identify the specific preparation to avoid having disorders personalized in localcharacteristics.Methods: This is a descriptive-analytic cross-sectional study. The research was carried out at Asri Medical Center in Yogyakarta, Indonesia, with a total sample size of 92 people who met the inclusion and exclusion criteria. They were divided into two groups: non-PCOS and PCOS. Data were retrieved using the questionnaire. The variables evaluated were nutritional status, physical activity, carbohydrate diet, fiber diet, family history of diabetes, family history of PCOS, and age of menarche. The data was analyzed usingthe Chi-square test.Results: Family history of PCOS, obesity, low physical activity and a lowfiber diet proved to differ significantly between the two groups (p<0.05).Conclusion: Family history of PCOS, obesity, low physical activity, low fiber diet had a substantial impact on the occurrence of PCOS.
Hypokalemic periodic paralysis in subclinical hyperthyroidism: A case report
Authors: Christabel Eunike Vania , Williyanto Onggo
Published: July 2023

Hypokalemia is a common electrolyte abnormality often encountered in daily practice. While mild hypokalemia often asymptomatic, moderate-to-severe hypokalemia usually manifested as significant symptoms such as muscle weakness. In this paper, we presented a case of 23-years-old male coming to our emergency department because of muscle weakness in all his four limbs when he woke up in the morning. Physical examinations were all within normal limit, except that his muscle strength decreased in all four extremities. Laboratory measures showed severe hypokalemia (1.9 mmol/L), hence patient was treated with potassium infusions. During hospitalization, thyroid function tests revealed hyperthyroidism. Therefore, in patient with paralysis, in which laboratory finding showed hypokalemia, hyperthyroidism should be considered as one of the potential diagnosis especially in younger patient, even in patient without previous history of hyperthyroidism. Merely treating hypokalemia in patient with underlying hyperthyroidism could be a dangerous boomerang.
Effect of salat on blood pressure and pulse rate in stage one primary hypertension patients
Authors: Legiran Legiran , Febriani Ratika , Mubarak Muhammad Ridho , Pakpahan Sandra
Published: July 2023

Background: Hypertension is included in 15 major causes of death in the United States. Primary prevention and prompt treatment of hypertension can prevent mortality. Previous research on salat revealed that prayer provides a feeling of relaxation and activates parasympathetic activation, affecting the cardiovascular system. Objective: This study aims to investigate the effect of salat on blood pressure (BP) and pulse in patients with stage one primary hypertension.Methods: This is a quasi-experimental study with a non-equivalent pre- and post-test control group design. The sample was taken by purposive sampling method. Thirty-six outpatients with stage one primary hypertension who met the inclusion and exclusion criteria were included. The data were analysed by the Friedman and Wilcoxon post-hoc follow-up tests.Results: The statistical analysis showed significant differences in systolic BP (SBP) at 5 minutes after salat, 10 minutes after salat, and 5 minutes vs 10 minutes after salat, both in the treatment and control groups. For the diastolic BP (DBP), a significant difference was found at 5 minutes after salat and 10 minutes after salat in the treatment group, as well as at 10 minutes after salat and 5 minutes after salat vs 10 minutes after salat in the control group. For the pulse variable, a significant difference was found at 5 minutes after salat and 10 minutes after salat in the treatment group, and 5 minutes after salat in the control group.Conclusion: There is a decrease in BP and pulse rate in patients with stage one hypertension after salat.
Analysis of C-reactive protein/albumin ratio as a predictor of mortality in sepsis patients
Authors: Hasanah Uswatun , Handayani Irda , Nurulita Asvin
Published: July 2023

Background: Sepsis is a leading cause of death among critically ill patients with infections. Abnormal levels of C-reactive protein (CRP) and albumin in sepsis patients have been shown to predict mortality. Combining the two markers can increase the predictive value of mortality.Objective: The aim of this retrospective cohort study was to determine the cut-off value for the CRP/albumin ratio that could be used to predict mortality in patients with sepsis. Methods: Secondary data from 63 medical records of sepsis patients treated in the ICU of RSUP dr. Wahidin Sudirohusodo Makassar from January 2018 to December 2020 was used. Statistical analysis was performed using the Kolmogorov-Smirnov and Mann-Whitney tests. The prognostic value of the CRP/albumin ratio was analyzed using the ROC curve to obtain a cut-off value. A p-value < 0.05 was considered significant. Results: The sample included 43 deceased and 20 living patients with sepsis. The CRP/albumin ratio was significantly higher in the deceased patients (15.4) than in the living patients (2.4) (p<0.001). The ROC curve analysis revealed a CRP/albumin cut-off value of 4.3 with a sensitivity of 81.4%, specificity of 75.0%, positive predictive value (PPV) of 87.5%, negative predictive value (NPV) of 65.2%, and an accuracy of 79.4%. Conclusion: The study suggests a significant relationship between the CRP/albumin ratio and mortality in patients with sepsis. A CRP/albumin ratio of 4.3 can be used as a predictor of mortality in sepsis patients.
Optimization Method for Minimum Mass of Permanent Magnet Excited Hia with Air-Gap Winding
Authors: Liu Longjian , Yu Kexun , Xie Xianfei
Published: July 2023

This paper proposes the optimization method for the minimum mass of permanent magnet (PM) excited homopolar inductor alternator (HIA) with air-gap winding used for high-speed driving system. The calculation results show that there is an optimal value for shell diameter and unit number of HIA to make the mass to be smallest. A 1 MW at 22000 rpm PM excited HIA is designed. The analytical results are verified by the FEM simulations. The losses, temperature rise, rotor stress and modal of the PM excited HIA are also analyzed. The calculation results show that the solid rotor eddy-current losses and the temperature rise of PM excited HIA are acceptable. Also the rotor strength is adequate, and the rotor system is a stiffness rotor, and it can safely operate. Finally, the comparison between PM excited HIA and permanent magnet rotor machine is discussed.
Mixing renewable energy with pumped hydropower storage: Design optimization under uncertainty and other challenges
Authors: Zisos Athanasios , Sakki Georgia-Konstantina , Efstratiadis Andreas
Published: July 2023

Hybrid renewable energy systems (HRES), complemented by pumped hydropower storage (PHS), have become increasingly popular amidst the increase of renewable energy penetration. This con-figuration is even more prosperous in remote regions that are typically not connected to the mainland power grid, where the energy independence challenge intensifies. This research focuses on the design of such systems, from the perspective of establishing an optimal mix of renewable sources that takes advantage of their complementarities and synergies, combined with the versatility of PHS. However, this design is subject to substantial complexities, due to the multiple objectives and constraints to fulfill, on the one hand, and the inherent uncertainties as well, that span over all underlying processes, i.e., external, and internal. In this vein, we utilize a proposed HRES layout for the Aegean Island of Sifnos, Greece, to develop and evaluate a comprehensive simulation-optimization scheme in deterministic and, eventually, stochastic setting, revealing the design problem under the umbrella of uncertainty. In particular, we account for three major uncertain elements, namely the wind velocity (natural process), the energy demand (anthropogenic process), and the wind-to-power conversion (internal process, expressed in terms of a probabilistic power curve). Emphasis is also given to the decision-making procedure, which requires a thorough interpretation of the uncertainty-aware optimization outcomes. Finally, since the proposed PHS uses the sea as the lower reservoir, additional technical challenges are addressed.
Upper Airway Characteristics and Morphological Changes by Different Mads in OSA Adult Subjects Assessed by CBCT 3D Imaging
Authors: Venza Nicolò , Malara Arianna , Liguori Claudio , Laganà Giuseppina , Cozza Paola
Published: July 2023

MAD is the gold standard therapy for mild/moderate OSA and is a second-choice treatment in patients with severe OSA not responding to CPAP. The aim of this study was to analyze the upper airway characteristics of OSA patients and the morphological changes in the upper airway, inducted by four different types of mandibular advancement devices, using CBCT 3D imaging. Twenty-two patients were recruited after OSA diagnosis with polysomnography. Four different customized and titratable MAD were used and an initial CBCT scan was obtained for each patient. After 6 months to the end of the MAD titration phase, all the subjects showed an AHI of < 5 or a decrease of 50% in AHI with MAD when compared with initial AHI and a second CBCT scan with MAD in situ was performed. An important improvement of the airway was observed (+33.76%) and a significant difference in the enlargement ratio between the posterior soft palate (+32.41%) and the posterior tongue (+36.96%) region was also found. The greatest increase in airway volume was achieved in patients treated with the MAD Forward and TAP (respectively +42.77% and + 41.63%). MAD therapy is effective to treat mild/severe OSA with increased upper airway volume. The design of the MAD can influence the effectiveness of the treatment.
Exploring the Pathogenesis and Mechanism-Targeted Treatments of Rosacea: Previous Understanding and Updates
Authors: Chen Chengqian , Wang Peiru , Zhang Linglin , Liu Xiaojing , Zhang Haiyan , Cao Yajing , Wang Xiuli , Zeng Qingyu
Published: July 2023

Rosacea is a chronic inflammatory skin disease characterized by recurrent erythema, flushing, telangiectasia, papules, pustules, and phymatous changes in the central area of the face. Patients with this condition often experience a significant negative impact on their quality of life, self-esteem, and overall well-being. Despite its prevalence, the pathogenesis of rosacea is not yet fully understood. Recent research advances are reshaping our understanding of the underlying mechanisms of rosacea, and treatment options based on the pathophysiological perspective hold promise to improve patient outcomes and reduce incidence. In this comprehensive review, we investigate the pathogenesis of rosacea in-depth, with a focus on emerging and novel mechanisms, and provide an up-to-date overview of therapeutic strategies that target the diverse pathogenic mechanisms of rosacea. Lastly, we discuss potential future research directions aimed at enhancing our understanding of the condition and developing effective treatments.
Dynamical Behaviours of Matter Wave Positons in Bose-Einstein Condensates
Authors: Manikandan K. , Serikbayev N. , Vijayasree S.P. , Aravinthan D.
Published: July 2023

In this investigation, we explore the existence and intriguing features of matter wave positons in a nonautonomous one-dimensional Bose-Einstein condensate (BEC) system with attractive interatomic interactions. We focus on the Gross-Pitaevskii (GP) equation/nonlinear Schrödinger (NLS)-type equation with time-modulated nonlinearity and trap potential, governing nonlinear wave propagation in the BEC. Our approach involves constructing second- and third-order matter wave positons using a similarity transformation technique. We also identify the constraints on the time-modulated system parameters that give rise to these nonlinear localized profiles. The study considers three distinct forms of modulated nonlinearities: (i) kink-like, (ii) localized or -like, and (iii) periodic. By varying the parameters associated with the nonlinearity strengths, we observe a rich variety of evolution behaviors in the matter wave positon profiles. These behaviors include stretching, curving, oscillating, breathing, collapsing, amplification, and suppression. Our comprehensive studies shed light on the intricate dynamics of matter wave positons in BECs, providing valuable insights into their behavior and characteristics in the presence of time-modulated nonlinearity and trap potential effects.
An Analysis of CFD-DEM with Coarse Graining for Turbulent Particle-Laden Jet Flows
Authors: Weaver Dustin Steven , Miskovic Sanja
Published: July 2023

This paper presents the results of simulations of particle-laden air-solid jet flow in long straight tubes using CFD-DEM, along with an analysis of coarse-graining. Although previous studies have used CFD-DEM for similar flows, these have typically been in a dilute flow regime where uncoupled simulations are accurate. However, fully coupled simulations can introduce issues, necessitating validation studies to ensure that all coupling parameters are effectively used and that the physics is accurately represented. This paper validates the simulations against two different experimental studies, with fluid Reynolds numbers between 10,000 and 40,000 and Stokes numbers between 5.6 and 50. Interestingly, the profiles of mean particle velocity exhibit fewer discrepancies as the Stokes number increases but more discrepancies for the root mean square velocity compared to the experiments. Particle number flux is consistent with the experiments after the nozzle exit. Coarse-graining is also applied to the same simulations, achieving relatively accurate results. However, as expected, scaling of contact collision frequencies, forces, and stresses cannot be achieved, meaning that coarse-graining may be useful for comparing designs or operating parameters on an industrial scale but falls short when measuring the total energy dissipation of one experiment.
Thermodynamical versus Logical Irreversibility: A Concrete Objection to Landauer’s Principle
Authors: Lairez Didier
Published: July 2023

Landauer’s principle states that the logical irreversibility of an operation, such as erasing one bit, whatever its physical implementation, necessarily implies its thermodynamical irreversibility. In this paper, a very simple counterexample of physical implementation (that uses a two-to-one relation between logic and thermodynamic states) is given that allows to erase one bit in a thermodynamical quasistatic manner (i.e. that may tend to be reversible if slowed down enough).
Optimal Layout Methods of Deep Chamber for Coal-gangue Separation Based on the Horizon of the Weak Stratum
Authors: Zhu Cheng , Yuan Yong , Sun Hanqing , Chen Zhongshun , Wang Wenmiao
Published: July 2023

The layout of deep chamber for coal-gangue separation (DCCS) based on the horizon of the weak stratum can affect the stability of its surrounding rocks. First, the work summarized the main structural characteristics of DCCS. Similar simulation tests were performed to analyze the deformation and failure law of surrounding rocks of DCCS under different horizons of the weak stratum. Then, 60 numerical simulation schemes were designed by considering the lateral pressure coefficient and the thickness and horizon of the weak stratum. A comprehensive evaluation method was proposed for the stability of surrounding rocks. The optimal layout methods of the DCCS were determined based on the thickness and horizon of the weak stratum in different ground stress fields. If the thickness of the weak stratum was large and lateral pressure coefficient λ < 0.6 or λ > 1, the stability of surrounding rocks was optimal to arrange the side of DCCS along the weak stratum. When 0.6 ≤ λ ≤ 1, the optimal approach for controlling surrounding rocks was to position the floor of DCCS along the weak stratum. If the thickness of the weak stratum was small and λ<0.6 or λ>1, the stability of surrounding rocks was optimal to arrange the side of DCCS near roof along the weak stratum. When 0.6 ≤ λ ≤ 1, the optimal approach for controlling surrounding rocks was to arrange the side of DCCS near the floor along the weak stratum. The results can provide an important theoretical reference for the spatial layout and support design of DCCS.
A Reduced Order Model for Monitoring Aeroengines Condition in Real-Time
Authors: Rodrigo Jose , Sanchez de Leon Luis , Montañes Jose L. , Vega Jose M.
Published: July 2023

A reduced order model is developed to monitor aeroengines condition (defining their degradation from a baseline state) in real-time, by using data collected in specific sensors. This reduced model is constructed by applying higher order singular value decomposition plus interpolation to appropriate data, organized in tensor form. Such data are obtained using a detailed engine model that takes the engine physics into account. Thus, the method synergically combines the advantages of data-driven (fast online operation) and model-based (the engine physics is accounted for) condition monitoring methods. Using this reduced order model as surrogate of the engine model, two gradient-like condition monitoring tools are constructed. The first tool is extremely fast and able to precisely compute `on the fly’ the turbine inlet temperature, which is a paramount parameter for the engine performance, operation, and maintenance, and can only be roughly estimated by the engine instrumentation in civil aviation. The second tool is not so fast (but still reasonably inexpensive) and precisely computes both, the engine degradation and the turbine inlet temperature at which sensors data have been acquired. These tools are robust in connection with random noise added to the sensors data and can be straight forwardly applied to other mechanical systems.
Research on Improved GRU-based Stock Price Prediction Method
Authors: Chen Chi , Xue Lei , Xing Wanqi
Published: July 2023

The prediction of stock prices holds significant implications for researchers and investors in evaluating stock value and risk. In recent years, researchers have increasingly replaced traditional machine learning methods with deep learning approaches in this domain. However, the application of deep learning in forecasting stock prices is confronted with the challenge of overfitting. To address the issue of overfitting and enhance predictive accuracy, this study proposes a stock prediction model based on GRU (Gated Recurrent Unit) with reconstructed datasets. This model integrates data from other stocks within the same industry, thereby enriching the extracted features and mitigating the risk of overfitting. Additionally, an auxiliary module is employed to augment the volume of data through dataset reconstruction, thereby enhancing the model’s training comprehensiveness and generalization capabilities. Experimental results demonstrate a substantial improvement in prediction accuracy across various industries.
Modified Citrus Pectin Treatment in Non-Metastatic Biochemically Relapsed Prostate Cancer: Long-Term Results of a Prospective Phase II Study
Authors: KEIZMAN DANIEL , Frenkel Moshe , Peer Avivit , Rosenbaum Eli , Sarid David , Leibovitch Ilan , Mano Roy , Yossepowitch Ofer , Wolf Ido , Geva Ravit , Margel David , Rouvinov Keren , Stern Anat , Dresler Hadas , Kushnir Igal , Eliaz Isaac
Published: July 2023

The optimal therapy for patients with non-metastatic biochemically relapsed prostate cancer (BRPC-M0) after local therapy is elusive. Thus, the evaluation of new non-toxic compounds in BRPC-M0 patients is warranted. PectaSol® modified citrus pectin (P-MCP) is a food supplement generally recognized as safe (GRAS) by the Food and Drug Administration (FDA) and is a competitive inhibitor of galectin-3 protein, involved in cancer pathogenesis. P-MCP treatment of BRPC-M0 patients for 6 months led to a 75% prostate-specific antigen doubling time (PSADT) improvement. To determine the longer-term effects of P-MCP we investigated an additional 12 months of treatment (phase II) with oral P-MCP at 4.8 grams X 3/day in patients with no disease progression in the previous 6 months. Of the 46 patients that entered the second phase, 7 patients withdrew consent and continued therapy independently, and 39 received another 12 months of therapy. After a total of 18 months P-MCP treatment, 62% (n=24) had a decreased/stable PSA, 90% (n=35) had improved PSADT, and 85% (n=33) had a durable long-term response, with a decreased/stable PSA, and/or improvement of PSADT, and negative scans. No patient had grade 3/4 toxicity. In conclusion, P-MCP may have long term durable efficacy, and is safe, in BRPC-M0.
Autoantigens of Nervous System and Human Coronavirus Antigens: Is There a Possibility for Molecular Mimicry?
Authors: Gavrilova Natalia Y. , Normatov Muslimbek G. , Soprun Lidia A. , Utekhin Vladimir J. , Fedotkina Tamara V. , Churilov Leonid P.
Published: July 2023

In post-COVID-19 syndrome, clinical presentation of the nerve fiber dysfunction plays an important role. The possibility of autoantigen cross-mimicry of coronavirus infection and the peripheral nervous system need to be investigated. The bioinformatic analysis was applied to search for possible common protein sequences located in the immunological epitopes. Among the autoantigens of the human nervous system, fibroblast growth factor receptor protein 3, myelin protein P0, myelin protein P2, sodium channel protein type 9, alpha protein subunit, plexin-D1 protein and ubiquitin-carboxyl-terminal hydrolase protein of the L1 isoenzyme were selected. The “Alignmentaj” program was created. The UniProt database, Protein Data Bank and AlphaFold databases were used. The analysis of protein sequence similarities of spike glycoproteins in human coronaviruses revealed common pentapeptides of the MERS-CoV-2 virus with the fibroblast growth factor receptor 3 and myelin protein P2. Among seasonal coronaviruses, common peptide sequences were identified in HCoV-HKU-1 virus with sodium channel protein type 9 subunit alpha and Plexin-D1, HCoV-OС43 with Plexin-D1, as well as HCoV-NL63 with Plexin-D1 and Ubiquitin carboxyl-terminal hydrolase isozyme L1. The data obtained make it possible to identify new potential targets for the development of autoimmune reactions that occur against the background of the activity of highly pathogenic and seasonal coronaviruses.
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 01.07.2023 г. по 07.07.2023 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: July 2023

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19 за неделю с 01.07.2023 г. по 07.07.2023 г.
Unlearning As Learning? A Critical Analysis of Student Veteran Support at a Veteran-Friendly Campus
Authors: Carter Carrie , Lim Jae Hoon , Interiano-Shiverdecker Claudia , Dahlberg Jerry
Published: July 2023

This phenomenological case study critically examined how cultural conflict between student veterans and faculty/staff influenced the efficacy of strategies for supporting student veterans at a veteran-friendly campus. Through 29 interviews with faculty, student affairs professionals, and student veterans, we analyzed (a) how lack of knowledge about student veterans’ military experiences made inclusive and effective student veteran support challenging; (b) how diverging perspectives about higher education as a privilege or earned service, informed by often contrary norms in the military and higher education, led to cultural tension between faculty/staff and student veterans; and (c) how faculty/staff’s position as “gatekeepers” and members of the dominant cultural group in higher education led to an ironic and harmful expectation that student veterans unlearn their military cultural dispositions/identity in order to succeed. This study suggests a need for greater military cultural awareness among faculty/staff, continued critical analysis of the culture and power dynamics that student veterans face navigating higher education, and reconceptualization of what it means to be a veteran-friendly college campus.
Sentiment Analysis of the COVID-19 Booster Vaccine with the Naïve Bayes Algorithm
Authors: Utari Dyah Retno
Published: July 2023

In early 2020, a new deadly virus was discovered that can spread quickly, called SARS-CoV-2 or Coronavirus Disease 2019 (COVID-19). Indonesia is a country with a relatively high number of survivors of COVID-19. The success of the Indonesian government in providing massive COVID-19 vaccinations can minimize the risk of death. The World Health Organization (WHO) stated it would revoke the Public Health Emergency of International Concern (PHEIC) status for COVID-19 in May 2023. However, many positive cases of Covid-19 were still found in Indonesia. Most of them are survivors who have not carried out complete vaccinations until they get a booster vaccine. This study analyzes public sentiment about booster vaccines in Indonesia using the Naïve Bayes classification algorithm. The results showed that the classification modeling accuracy had an excellent value of 97.51%. In contrast, based on the analysis results, the number of words that frequently appeared in the twelve highest word cloud visualization results found tokens that had positive sentiment values.
Utilization of BCFS (Biomass Coal Fuel-Sludge) As Substitution of Energy Sources in Industrial Boilers
Authors: Arifin Danang Nor , Hidawati Hidawati
Published: July 2023

The utilization of coal as an energy source is still the main choice of the textile industry in Indonesia. The process of this utilization left 20% of FABA (fly ash-bottom ash) where 80% of them were bottom ashes which are considered hazardous and toxic waste. This study aims to obtain the technology and production process for treating bottom ash and biomass from urban waste as an alternative energy source and reducing environmental problems (zero waste: waste to energy and waste to material). Biomass Coal Fuel-Sludge (BCFS) Briquettes as a product derived from bottom ash, biomass, and biosludge had a composition of 50%: 30%: 20%. The briquettes utilization can reduce coal use by 16% and increase boiler temperature to 35°C - 75°C. The emission in the trial burning test which were the particulates, sulfur dioxide, and nitrogen dioxide met the quality standards required in the Technical Approval in the Sector of Hazardous Waste Management from the Ministry of Environment and Forestry. With this research, the BCFS briquette products can be a solution to the utilization of B3 waste and waste problems.
Design a Car Sales Business Intelligence Using the K-Means Algorithm With Holt-winter's Smoothing Method
Authors: Basri K Ilham , Abdilah David Fahmi , Galahartlambang Yanuangga , Rosid Mochammad Alfan
Published: July 2023

The use of Business Intelligence (BI) systems as decision support systems in the automotive environment, especially luxury cars, is still limited to support decision making regarding production and distribution policies. Forecasting is an important tool in planning for every organization and agency in need. The purpose of this study is to design BI that can be applied to luxury car show rooms as part of the automotive business organization by utilizing existing information systems. This research can also be used to see an overview of luxury car sales patterns using clustering techniques using K-Means algorithms. This study was designed by relating data on fuel increases from previous years. The results of this study are in the form of forecasting data using the smothing holtwinters method of luxury car sales in the next 5 years by adjusting to the conditions of fuel increase in the next 5 years for the process of making predictions of luxury car needs in Indonesia in the coming period.
PROTOCOL OF ANTICOAGULATION IN EXTRACORPOREAL PURIFICATION TECHNIQUES
Authors: González-Fernández María , Quílez-Trasobares Nerea , Barea-Mendoza Jesús A. , Molina-Collado Zaira , Arias-Verdú Dolores , Barrueco-Francioni Jesús , Seller-Pérez Gemma , Herrera-Gutiérrez Manuel E. , Riera José Ángel Sánchez-Izquierdo
Published: July 2023

The need for anticoagulation associated with the use of extracorporeal purification techniques is one of the main concerns regarding these techniques. Although we use sodium heparin (500 IU/h) as the main anticoagulant, we assess the presence of contraindications to it, mainly associated with bleeding risk (thrombocytopenia, coagulopathy). We consider alternatives such as the use of epoprostenol or citrate, or performing EPT without anticoagulation. Furthermore, when repeated hemofilter clotting occurs (>2 sets/day), we consider changing the anticoagulation system, preferably to citrate. While we need to monitor bleeding complications when using heparin or epoprostenol, these complications are minimized with citrate as it is a regional anticoagulation system. In the latter case, we need to monitor for ionic disturbances (mainly hypocalcemia and hypomagnesemia) and electrolyte imbalances (metabolic alkalosis or acidosis due to citrate accumulation) that may occur if adequate monitoring is not carried out.
Design and development of a novel Crystal ribcage for probing real-time lung function at cellular resolution in health and disease
Authors: Banerji Rohin , Grifno Gabrielle N. , Nia Hadi T.
Published: July 2023

Multiple diseases impact the mechanical, biological, and immune function of the lung at the cellular scale. Despite recent progress in intravital imaging, optical imaging of the lung during active respiration and circulation has remained challenging. We introduce here the crystal ribcage: a transparent ribcage that (i) allows truly multiscale optical imaging of the lung in health and disease from whole-organ to single cell, (ii) enables modulating the lung biophysics and immunity through intravascular, intrapulmonary, intraparenchymal, and optogenetic interventions, and (iii) preserves the 3-D architecture, air-liquid interface, cellular diversity, and respiratory-circulatory functions of the lung. We probed how diseases like cancer and pneumonia progression remodels the respiratory-circulatory functions at the single alveolus and capillary levels. The crystal ribcage and its broad applications will facilitate further studies of real-time remodeling of the alveoli and capillaries during pathogenesis of nearly any pulmonary disease, leading to the identification of new targets for treatment strategies.
In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Authors: Vatandaslar Hasan , Tuschl Tom , Stoffel Markus
Published: July 2023

Here, we describe viP-CLIP (in vivo PAR-CLIP) that is capable to identify endogenous RBP targets in mammalian tissues, thereby facilitating the functional analysis of RBP-regulatory networks in vivo. Briefly, mice are exposed to 4-Thiouridine that is incorporated into newly transcribed RNAs in the organism, enabling an efficient UV-crosslinking in multiple tissues crucial for a successful experiment. We report a comprehensive protocol of the critical steps to adapt the cell culture PAR-CLIP1 protocol to the viP-CLIP protocol carried out in mice.
Exploratory Data Analysis of Earthquake Moment Tensor Catalog using Non-linear Graph-based Dimensionality Reduction
Authors: Kubo Hisahiko , Kimura Takeshi , Shiomi Katsuhiko
Published: July 2023

The information on spatial location, origin time and fault mechanism type of earthquakes is contained in moment tensor solutions, which are routinely estimated. The catalog of moment tensor solutions has been interpreted by researchers based on a wealth of past knowledge; however, the long-term routine analyses have led to the accumulation of a huge amount of data in moment tensor catalog, and it is necessary to move away from the artisanal approach. In this study, using dimensionality reduction of unsupervised machine learning, we performed exploratory data analysis of the moment tensor catalog to objectively obtain images of seismic activity and to acquire knowledge on the spatial and temporal characteristics of the earthquake mechanism. Source parameters of the moment tensor catalog in Japan, spatial location (latitude, longitude, and depth) and source-mechanism diagram information, were embedded in two-dimensional space via a non-linear graph-based dimensionality-reduction method, Uniform Manifold Approximation and Projection. On the embedding map, earthquakes in eastern and western Japan are distributed separately and are further embedded to reflect their characteristic fault mechanism and depth in each region. The similarity degree of the earthquakes can be obtained as the distance on the embedding map. This study demonstrates that the data visualization using dimensionality reduction is useful for intuitively and objectively understanding the regional characteristics of earthquake mechanisms. The embedding map can also be employed to visualize temporal changes in regional seismic activity, and to perform a similarity search with a past event.
Optimization of sunlight photo-catalytic treatment coupled to a coagulation-flocculation process
Authors: Raissi Sahar , fakhfakh fatma , Hadrich bilel
Published: July 2023

The oleic industry is one of the most polluting in food industry sector. An easy treatment of this waste is Coagulation–flocculation. Photo-catalysis can be used as complementary treatment to improve the water quality and make it reused. We optimize this method to treat mill wastewater collected from the region of Mahdia, by varying the conditions and studying their effects. Organic pollutant were removed and the final quality of water were under the imposed norm NT 106-02.
Creation of a Favorable Antitumor Microenvironment by the Anti-Complement Factor H Antibody mGT103
Authors: Saxena Ruchi , Bushey Ryan T. , Campa Michael J. , Gottlin Elizabeth B. , Guo Jian , Patz Edward F. , He You-Wen
Published: July 2023

Complement factor H (CFH) is an abundant serum protein regulating complement activation and protecting host cells from attack by the alternative pathway of complement mediated cytotoxicity. We previously identified an anti-CFH autoantibody in early-stage NSCLC patients, and cloned from single peripheral B cells a lead therapeutic mAb, GT103, for cancer immunotherapy. Although GT103 has potent antitumor activities, the underlying mechanisms remain unknown. Herein we investigated GT103-mediated antitumor effects and demonstrate that murine GT103 (mGT103) enhances antitumor immunity through multiple pathways. It creates a favorable tumor microenvironment (TME) by decreasing immunosuppressive regulatory T cells and myeloid-derived suppressor cells and enhancing antigen-specific effector T cells, and has a synergistic antitumor effect with anti-PD-L1 mAb. mGT103, previously shown to activate complement in vitro and in vivo, requires complement for its activity in vivo. Furthermore, the immune landscape of tumors from early-stage patients expressing the anti-CFH autoantibody is associated with an immunologically active TME. Our results provide novel mechanistic insights into this promising human-derived immunotherapeutic agent, which is currently undergoing a phase Ib clinical trial as monotherapy and a phase II combination trial with Keytruda for advanced, refractory/relapsed NSCLC patients.
Spectroscopic properties of dissolved organic matter during the wet season in the Huma River Basin, China
Authors: Zhang Daoping , Meng Fansheng , Zhang Lingsong , Wang Yeyao , Xue Hao , Liang Zhuming , Zhang Jiasheng
Published: July 2023

Dissolved organic matter (DOM) is the largest organic matter pool in natural waters, which is critical for ecological and societal values. The Huma River Basin (HRB) in China during the wet season presented a high background value, owning to the unusually high CODMn concentrations. To explain the phenomenon, the spectroscopic properties of DOM were studied using spectroscopic techniques combined with statistical analysis. The variations of several UV-visible parameters showed that DOM predominantly consisted of aromatic and high-molecular-weight natural organic matter. Fluorescence excitation-emission matrixes (EEMs) spectroscopy-parallel factor analysis (PARAFAC) recognized four characteristic components representing humic-like substances (C1, C2 and C3) and protein-like substances (C4). Fluorescence parameters showed that the allochthonous terrestrially-derived DOM had important humic character. Fourier transform infrared (FT-IR) spectra characterized the structure of DOM, containing aromatic, aliphatic, carbohydrate and protein compounds. Principal component analysis (PCA) revealed that humic-like components explained approximately 93.8% of the total variance. Correlation coefficient matrix analysis indicated that DOC and CODMn were mainly derived from humic-like substances. The results demonstrated that the background value in the HRB during the wet season was mainly attributed to terrestrial humic-like substances. Such knowledge could assist in monitoring and managing the river with a high background value.
N6-Methylandenosine-Related lncRNAs are Potential Biomarkers for Predicting Prognosis and Immune Response in Kidney Renal Clear Cell Carcinoma
Authors: Meng Chang , Li Juan , Wang Xiang , Ying Yicen , Li Zhihua , Wang Aixiang , Li Xuesong
Published: July 2023

Both lncRNAs and N6-Methylandenosine (m6A) modification have been reported as key regulators in tumorigenesis and innate immunity. This study is aimed to develop a prognostic signature based on m6A-related lncRNAs in kidney renal clear cell carcinoma (KIRC). Differential expression analysis and Pearson correlation analysis were used to identify m6A-related lncRNAs in The Cancer Genome Atlas (TCGA) database. The least absolute shrinkage and selection operator (LASSO) Cox regression analysis was applied for further selection and the selected genes were inputted into stepwise regression to develop m6A-related lncRNA risk score (MRLrisk). According to our results, MRLrisk was established based on 6 m6A-related lncRNAs, NFE4, AL008729.2, AL139123.1, LINC02154, AC124854.1 and ARHGAP31-AS1. Higher MRLrisk was identified as a risk factor for patients' prognosis in TCGA dataset as well as in subgroup analysis with different clinicopathological characteristics. Furthermore, a MRLrisk-based nomogram was developed and demonstrated as a reliable tool for prognosis prediction in KIRC. MRLrisk-related biological phenotypes were analyzed in enrichment analysis and tumor mutation signature, providing us with novel insights for further functional studies. Additionally, patients' response to immunotherapy was inferred by the tumor immune dysfunction and exclusion (TIDE) score. Results showed that higher MRLrisk may indicate worse response to immunotherapy. pRRophetic R package was used to predict patient's response to certain chemodrugs and targeted drugs. In conclusion, we developed a MRLrisk model with robust prognostic value and ability to predict immunotherapy and targeted therapy response in KIRC, which may contribute to clinical patient stratification and treatment selection for KIRC.
Effect of low-dose esketamine combined with labour analgesia on postpartum depression in spontaneous labour: a prospective case-control study
Authors: Gao Jianxin , Dai Lan , Duan Baiqing , Peng Dan , Huang Xi , He Liyunjian , Zou Jingni , Zhou Qin , Miao Dan
Published: July 2023

Purpose To evaluate the effect of a single intravenous low-dose esketamine combined with labour analgesia on the occurrence of postpartum depression in patients with spontaneous labour.Methods Female patients in labour were divided into a group of esketamine combined with labour analgesia (group A, n = 116), a group with labour analgesia alone (group B, n = 132) and a control group (group C, n = 51) according to the mode of analgesia. The Edinburgh Postpartum Depression Scale (EPDS) and visual analog scale (VAS) scores were collected at different time points for all three groups. Serum was also collected from patients before and after delivery to detect serum sex hormone changes.Results This clinical study was a prospective, randomised, double-blind trial. A total of 299 patients were enrolled in the study. Cross-sectional analysis showed no significant differences in EPDS scores or incidence of depression between the three groups in postpartum period, or at 1, 7 or 42 days postpartum. There were no statistically significant differences in VAS scores at 2 hours postpartum, 1 day, 2 days and 7 days postpartum. No significant differences were seen in the levels of oestrogen, progesterone, 5hydroxytryptamine and serum cortisol before delivery between the three groups. After delivery, serum cortisol levels were higher in the labour analgesia alone group than in the esketamine combined with labour analgesia group and the control group (P < 0.001). Longitudinal analysis showed that EPDS and VAS scores improved significantly over time in postpartum period for both combined esketamine and labour analgesia alone (P < 0.001), but no significant change was seen in the control group. This improvement was possibly associated with a decrease in postpartum oestrogen levels and an increase in serum cortisol levels (P < 0.001).Conclusion In this study, the combination of esketamine with labour analgesia did not reduce the incidence of depression and VAS scores at 1, 7 or 42 days postpartum.
Physicians’ attitudes towards patients with substance-related disorders predict training needs in addiction medicine: challenges and opportunities for strengthening the global addiction medicine workforce
Authors: Pinxten W. J. Lucas , Fitriana Efi , Jokūbonis Darius , Adomaitiene Virginija , Leskauskas Darius , Jong Cornelis A.J. , Hutschemaekers Giel J.M.
Published: July 2023

Introduction
Substance use disorders (SUD) are increasing in prevalence, and most physicians will encounter patients with SUD (PWSUD). As a result, the training of the global addiction workforce requires expansion. Two primary barriers to improving the quality and quantity of the global addiction workforce are healthcare providers' negative attitudes toward PWSUD and a lack of qualified addiction physicians. The authors are unaware of any published research on the association between these two barriers: a study that could help inform us about how to strengthen global addiction services through targeted medical training.
Methods. The MCRS, measuring professionals' attitudes towards patients with SUD, and the AM-TNA, measuring perceived training needs and competence gaps, were used to study the association between attitudes and competence. We used Pearson correlation, multiple regression, and PROCESS path analysis in a cross-sectional study design in a convenience sample of addiction physicians trained in The Netherlands (Dutch)(N = 70) and in Lithuanian (Lithuania) (N = 75) to explore this association and a possible moderation effect of the Gender and Country of Origin variables among addiction caregivers.
Results. We found a significant positive correlation between the total score of both scales: R = 0.537, p < 0.001. The MCRS predicted the three AM-TNA subscales significantly p < 0.001, and Gender did not influence the association between the two scales. The Country of Origin variable and its combination with the MCRS total score did strengthen the association between the MCRS and two AM-TNA subscales: medical- ( p < 0.001) and practical aspects of perceived addiction competence ( p < 0.05 ).
Conclusions. Attitudes predict perceived training needs, meaning addiction physicians with positive attitudes are likelier to have and want better-developed addiction knowledge and skills. To improve addiction services, the in-tandem use of the MCRS and the AM-TNA could reveal specific SUD competence gaps and model the design of an integrated addiction training or intervention approach.
Future research should focus on the longitudinal use of the combined scales, assess individual professionals’ progress, and compare and combine self-reported competencies and attitudes with validated external assessments of learning, attitudes, and self-efficacy.
Indoor environment in relation to recurrent childhood asthma in China:A Hospital-based case-control study
Authors: Zhang Baoping , Xia Zhibing , Jiang Xu , Yin Chuntao , Yuan Yang , Chen Tianming
Published: July 2023

Background:
This investigation explored the association between indoor environmental factors and childhood asthma in Yancheng, China.
Methods:
Asthma case (201 children with recurrent asthma) and control cohorts (242 healthy subjects) were recruited from a Traditional Chinese Medical (TCM) Hospital in Yancheng city, based on the results of an ISAAC questionnaire. Questionnaires regarding environmental risk factors were completed by the child’s primary caregivers. To compare data on environmental VOCs and formaldehyde contents between asthma and control cohorts, we passively conducted a 10-day indoor and outdoor sampling.
Results:
Breastfeeding was a major protective indoor environmental factor for recurrent asthma (adjusted odds ratio [aOR]: 0.368, 95% confidence interval[CI]: 0.216-0.627). Our analysis revealed that childhood recurrent asthma was intricately linked to a family history of asthma. Recurrent asthma was also associated with passive smoking [aOR2.115 (95%-CI 1.275-3.508)]. Analogous correlations were observed between household renovation or new furniture introduction and recurrent asthma [aOR3.129(95%-CI1.542-6.347)]. Benzene and formaldehyde were present in all examined homes. Enhanced benzene and formaldehyde concentrations were strongly evident among asthma versus control cohorts, and they were strongly correlated with augmented recurrent asthma risk.
Conclusion:
Home environment heavily regulates incidences of childhood recurrent asthma. Hence, actions against the indoor environmental risk factors described in this study may assist in the prevention of recurrent asthma among children.
Inoculating exogenous bacterium Brevibacillus laterosporus ZR-11 in compost-maturing period could accelerate composting maturation by regulating physicochemical parameters and bacterial community succession
Authors: Ren Li , Li Jieming , Li Huifen , Guo Zhonghui , Li Ji , Lv Yizhong
Published: July 2023

In this experiment,Brevibacillus laterosporusZR-11, a bio-control strain with resistance to Fusarium wilt in cucumber, was inoculated in the mature manure prepared for maturing composting; conventional detection and Illumina Miseq high-throughput sequencing were adopted to investigate the changes of physicochemical factors and microbial communities in the composting process; correlation analysis was also conducted, with the purpose of providing some reference for improving the production technology of biological manure and upgrading the quality of products. According to experimental results, in the maturing period, bacterial community structures underwent substantial evolutions in both the inoculated group and the non-inoculated group Microbial diversity analysis revealed that Firmicutes, Proteobacteria, and Actinobacteria were dominant bacterial phyla in both the inoculated group and the non-inoculated group in different period, but their relative abundances were higher in the former. Through analyzing physicochemical indicators, it was found that ZR-11 could increase the temperature of maturing composting pile, accelerate compost maturation, and enhance the abundance of bacterial communities in the pile. The results of this study provided some reference for improving the maturing ING composting production technology of biological manure and upgrading the quality of products.