bluuebunny/crossref_metadata_2025 huggingface dataset:
Gravity currents with large salinity in a gravel bed valley: an experimental study
Authors: Keramaris E. , Vasileiou C. , Sagiannis K.
Published: March 2022

In the current study experiments in a valley (a composite cross-sectional tank consisting of a rectangular upper cross section and a lower trapezoidal) were performed. The purpose of this research is to study the gravel bed in gravity currents with large salinity. The tank is separated in two parts by a vertical gate, which separates the fresh water with the gravel bed from the salt water with the impermeable bed. When the gate opens, a flow is created due to density difference. The salinity densities are large (from 1,050 to 1,090 kg/m3). These specific densities have been chosen to simulate liquid wastes that affect the environment. Compared to experiments in the same tank without a gravel bed, it is observed that the gravel bed reduces the propagation of currents and mainly reduces the current with the smallest density difference at the lowest height. The main conclusion of this study is that the kind of channel bed plays a key role in the propagation of gravity currents. The gravel bed affects significantly the velocity and the distance traveled by the currents in comparison with impermeable bed.
Festas Religiosas no Espaço Virtual
Authors: ALVES MARIA LUCIA , ALVES MARIA LUCIA , Azevedo Lima de Barros Aline Gisele , Melo Santos Adriana , Melo Santos Adriana , Marques da Silvia Sylvana Kelly
Published: March 2022

O artigo resulta da análise de duas festas religiosas no estado do Rio Grande do Norte: Santa Rita de Cássia e Protomártires do Brasil que, já estruturadas em suas dinâmicas, re-organizaram seus espaços, deslocando as celebrações para os meios digitais - devido ao isolamento social provocado pela pandemia do Coronavírus (SARS-CoV-2).  O objetivo é analisar as festividades no espaço virtual ligadas à cultura religiosa transmitida de geração em geração. Trata-se de um estudo de caso múltiplo com abordagem qualitativa resultante da observação, questionários via comunicador instantâneo (messenger), participação em live, eventos online e discussões no meio virtual. Questiona-se, quais alterações as festas religiosas do catolicismo popular sofrem ao se deslocar para o ambiente virtual?
Probing the Interaction of Selonsertib with Human Serum Albumin: In
silico and In vitro Approaches
Authors: Baig Mohammad Hassan , Gupta Preeti , Khan Mohd. Imran , Alajmi Mohamed F. , Hussain Afzal , Hassan Md. Imtaiyaz , Dong Jae-June
Published: March 2022

Introduction:

Selonsertib, the most recently developed selective inhibitor of apoptosis signal-regulating kinase 1. We elucidated the binding characteristics, mechanism of interaction, and dynamic behaviors of selonsertib with human serum albumin (HSA), a major circulatory transport protein.

Method:

Different biophysical approaches (fluorescence quenching and isothermal titration calo-rimetry (ITC) were combined with various in silico techniques to examine the binding of selonsertib to HSA. Molecular docking results, analysis of molecular dynamics trajectories, and essential dy-namics investigations indicated the stable binding of selonsertib to HSA. Further in vitro studies were performed to validate the observed interaction.

Result:

ITC results confirmed the robust binding and high affinity of selonsertib and HSA. Like-wise, the fluorescence quenching results highlighted the binding affinity of selonsertib and HSA. Collectively, our findings offer deeper insight into the binding mechanism of selonsertib and HSA, emphasizing the selonsertib-mediated structural changes within HSA, along with a comprehensive rationale for the biological transport and accumulation of selonsertib in the blood plasma.

Conclusion:

Therefore, considering the bioavailability and effectiveness of selonsertib, assessing the interactions of this inhibitor with carrier proteins is crucial to elucidate its biological processes at the molecular level. This evidence carries the considerable scientific potential for future drug de-sign.
Recent Developments of Nanomaterials and Sensor Performance for
Electronic Skin
Authors: Xu Ke , Su Rong
Published: March 2022

Throughout the research of flexible nanomaterials and sensing technology in recent years,
electronic skin has been widely developed as well as applied in many fields. As a bionic flexible
tactile sensor, electronic skin can simulate the touching of human skin with external signals as well
as collect and detect dynamic information of the physical surface. This paper reviews the flexible
substrate materials and electrode nanomaterials of electronic skin. The stable support of the flexible
substrate largely determines the mechanical properties of the electronic skin. At the outset, this article
introduces the flexible substrate materials commonly used in electronic skins. PDMS, PI, and
PET are typical representatives of flexible substrate materials. Then, the nanomaterials used for electrodes
are discussed, including one-dimensional and two-dimensional nanomaterials, especially emphasizing
the innovation of the sensor performance about the advanced electronic skin along with the
use of different nanomaterials under the integrated application background. In addition, these electrode
nanomaterials need to be appropriately embedded in flexible substrate materials. The response
time, sensitivity, detection limit, response range, and the cycle of electronic skin are selected for
comparison. Finally, the opportunities and challenges of electronic skin in nanomaterials and sensing
technology are summarized.
Black Fungus: An Alarming Infection During Novel Coronavirus: A Review
Authors: Das Raneet , Roy Jayita , Ashique Sumel
Published: March 2022

Mucormycosis is the most emerging angioinvasive fungal infection of filamentous fungi of the
Zygomycetes class, which, when neglected, causes severe disseminated infection along with significant
chances of morbidity and mortality. The diagnosis and treatment remain challenging for the doctors. It
has been observed that people who have been suffering from different diseases, such as hematological
malignancies and uncontrolled diabetes, or who have gone through different surgeries, such as hematopoietic
stem cell transplant, and solid transplantation, are the most affected ones. On the other hand, people
who have recovered from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) typically
show the symptoms of mucormycosis after 1-2 weeks of successful recovery. Standard treatment of mucormycosis
is traditionally considered an amphotericin B intravenous (IV) drug as initial therapy, although
posaconazole and isavuconazole are also used. The core objective of the review is to typically
focus on the area of the sudden cause of mucormycosis in the patients who have already recovered from
SARS-CoV-2.
Morphological Characterization and Antioxidant Activity of Actinobacteria from Xylocarpus granatum Growing in Mangrove Habitat
Authors: Ratte Marina , Batubara Irmanida , Lestari Yulin
Published: March 2022

Xylocarpus granatum produces various bioactive compounds with diverse biological activities, one of them as an antioxidant. Endophytic actinobacteria can also produce bioactive compounds. This study aimed to explore the existence of the endophytic actinobacteria of X. granatum fruit and determine its antioxidant activity. The research method was carried out by isolating the endophytic actinobacteria from the fruit using selective media Humic Acid (HV). Furthermore, characterizing the obtained isolates using four different media, namely YSA, ISP2, ISP3, and ISP4, measured antioxidant activity for actinobacterial supernatants and actinobacterial ethyl acetate extracts using the DPPH method. The results showed that from X. granatum, 15 isolates of endophytic actinobacteria were obtained from fruit, seeds, and leaves. Endophytic actinobacteria isolates from X. granatum showed different colors of aerial mycelium, substrate mycelium, and spore chain type. XGF11, XGF12, and XGF4 isolates produced high antioxidant activity of the supernatant; 57.30%, 49.77%, and 41.90% inhibition, respectively. The antioxidant capacity of XGF12 ethyl acetate extract was 501.60 μg AEAC/mg extract and 465.47 μg AEAC/mg extract for XGF11 ethyl acetate extract. Based on 16S rRNA gene analysis, isolates XGF12 and XGF11 showed similarities with Streptomyces xylanilyticus, and isolated XGF4 showed similarities with Brevibacterium sediminis.
Intestinal Microbiota and Sclerosis Lateral Amyotrophic
Authors: Vilar Mariana Dantas de Carvalho , Vale Sancha Helena de Lima , Rosado Eliane Lopes , Dourado Júnior Mário Emílio Teixeira , Brandão-Neto José , Leite-Lais Lúcia
Published: March 2022

The human gastrointestinal tract contains numerous microorganisms. This intestinal microbiota (IM) has a mutualistic relationship with the human organism, and it plays a fundamental role in regulating metabolic, endocrine, and immunological functions. Intestinal dysbiosis is associated with phenotypes of many chronic and inflammatory diseases. This association is explained by the functions of the IM and the existing bi-directional communication of the microbiota-intestine-brain axis. Studies have uncovered new evidence between the IM and neurodegenerative diseases recently, including amyotrophic lateral sclerosis (ALS). Given this, the present narrative review discusses didactically about IM, its functions, its relationship with the neuroimmune-endocrine system, and its association with neurodegenerative diseases, with emphasis on ALS.
Dietary Protein and Its Associations with Cardiovascular Health
Authors: Reddy Koushik , Freeman Andrew M. , Ostfeld Robert , Aspry Karen , Allen Kathleen , O'Keefe James , Ornish Dean , Batts Travis , Aggarwal Monica , White Beth , Patel Hena , Klodas Elizabeth , Levin Susan , Lee Dae Hyun , Williams Kim A.
Published: March 2022

An adequate intake of dietary protein is critical to human health and, as a result, protein is the only macronutrient with a recommended daily allowance. Yet, compared to fat and carbohydrate sources, the impact of dietary protein sources on human and cardiovascular health has received far less attention. However, consistent data from large prospective cohort studies from the United States, Europe, and Asia have consistently documented reduced risk of ischemic heart disease (IHD) in individuals with the highest consumption of plant proteins, and an increased risk of IHD in those with the highest intake of animal proteins. Which cardiovascular disease (CVD) risk factors are most impacted is less clear. Mechanistic studies are limited by the fact that, like other macronutrients, protein in the human diet comes from a myriad of sources with varying proportions of non-protein micro and macronutrients that have independent influences on the development of atherosclerotic cardiovascular disease (ASCVD). However, growing evidence suggests that both non-protein and protein components of plant vs. animal protein sources may confer ASCVD protection or risk, and that inflammation and the gut microbiome play crucial roles.
A Resistência Institucional pelos Direitos Humanos: a atuação da Associação Brasileira de Imprensa e a Ordem dos Advogados do Brasil no Conselho de Defesa dos Direitos da Pessoa Humana (1968-1985)
Authors: Da Silva Leonardo Fetter
Published: March 2022

Criado em 1964, o Conselho de Defesa dos Direitos da Pessoa Humana foi instalado em 1968, tendo como objetivo promover e defender os direitos humanos no Brasil e possuindo uma composição que incluía representantes do governo e entidades civis. O presente artigo propõe discutir e analisar a atuação da Associação Brasileira de Imprensa e a Ordem dos Advogados do Brasil dentro do Conselho e suas posturas perante a ditadura civil-militar, por meio de fontes oficiais e jornalísticas. Assim, destaca-se como as entidades passaram a incorporar na década de 1970 a luta e defesa dos direitos humanos como forma de resistência contra a ditadura e praticar uma resistência institucional por meio do Conselho.
Elites políticas e gestão de diretórios partidários no Brasil Monárquico: a reorganização administrativa do Partido Conservador (1867)
Authors: Gomes Sandro Aramis Richter
Published: March 2022

Neste artigo é desenvolvida uma investigação sobre o projeto de reorganização administrativa do Partido Conservador, o qual data de 1867. Há três argumentos fundamentos neste trabalho. Primeiro, trata-se de demonstrar que na época anterior a essa reorganização existiam marcantes diferenças quanto à estrutura e ao funcionamento das unidades regionais do Partido Conservador. Segundo, é evidenciado que, em relação à reestruturação do Partido Liberal, a reorganização do Partido Conservador foi concebida por grupo politicamente menos homogêneo. Nesse contexto, houve menor interferência dos senadores conservadores nas mudanças administrativas efetuadas na agremiação. Terceiro, cumpre mencionar que tais mudanças não reduziram os poderes das elites provinciais para gerirem localmente o partido.
MULHERES NO FIO DA NAVALHA: FEMINICÍDIO, BARBÁRIE E PEDAGOGIA DA CRUELDADE
Authors: De Sá Pereira da Silva Rosária de Fatima
Published: March 2022

Este artigo pretende elucidar o feminicídio enquanto um fenômeno estrutural e atinente ao movimento histórico do capital, no qual para sua compreensão, incorporaremos a análise, a partir dos seguintes eixos: 1)  Apresentação do debate conceitual sobre as relações de gênero e violência; 2) Exame do processo de constituição do patriarcado moderno e problematização e reconhecimento do feminicídio enquanto um fenômeno sustentado pelo capitalismo e pelo patriarcado; 3) Análise dos dados sobre mortalidade violenta de mulheres no Brasil.
DE BRUCE A CAITLYN JENNER: ESPORTE, CELEBRIDADE, TRANSGENERIDADE
Authors: Vaz Alexandre Fernandez , Camargo Wagner Xavier de , Silva Keo , Silvestrin Julian Pegoraro
Published: March 2022

Políticas identitárias e identidades transgênero têm mobilizadoopinião pública, mídias e mundo científico. Afinal, o que um corpo transgênerodiz sobre si? Partindo disso, tomamos a trajetória de Bruce/Caitlyn Jennercomo mote analítico. Para além da construção imagética da celebridade e desua trajetória como ex-atleta, Caitlyn postula novas possibilidades de repensaras relações com o próprio corpo transgênero e outros corpos. Entendendo quecorpo, política e esporte não estão descolados, ao atrelarmos performanceesportiva à performatividade de gênero discorremos acerca de impedimentose regulações sobre o corpo transgênero no esporte. Sintetizamos, finalmente,questões para o esporte e a sociedade.
Persepsi dan Sikap Calon Guru Biologi Terhadap Potensi Instagram Sebagai Sarana Edutainment dalam Pembelajaran Biologi
Authors: Utami Aprillyana Dwi , Kurniasiih Mike Dewi
Published: March 2022

The purpose of this study was to examine prospective biology teachers’ perceptions and attitudes towards Instagram as a potential edutainment tool in biology learning. The study involved 87 prospective biology teachers from 4 public universities in Indonesia. Participants answered 15 closed questions and three open questions. Then data were analysed quantitatively and qualitatively. The results demonstrated that prospective biology teachers had highly positive perceptions of Instagram as an edutainment tool in biology learning. Respondents strongly agree that Instagram is easier to use than other learning platforms to create and upload learning content. Prospective biology teachers’ attitudes show that they agree to use Instagram as an edutainment tool in biology learning. The use of Instagram also has the potential to increase motivation, interaction, and creativity and is enjoyable in learning biology.
Key words: Instagram, edutainment, perceptions, prospective biology teacher
 
Abstrak. Tujuan dari penelitian ini adalah untuk menguji persepsi dan sikap calon guru biologi terhadap Instagram sebagai sarana edutainment yang potensial dalam pembelajaran biologi. Penelitian ini melibatkan 87 calon guru biologi dari 4 perguruan tinggi negeri di Indonesia. Partisipan menjawab 15 pertanyaan tertutup dan tiga pertanyaan terbuka. Kemudian data dianalisis secara kuantitatif dan kualitatif. Hasil penelitian menunjukkan bahwa calon guru biologi memiliki persepsi yang sangat positif terhadap Instagram sebagai sarana edutainment dalam pembelajaran biologi. Responden sangat setuju bahwa Instagram lebih mudah digunakan dibandingkan platform pembelajaran lain untuk membuat dan mengunggah konten pembelajaran. Sikap calon guru biologi menunjukkan bahwa mereka setuju untuk menggunakan Instagram sebagai sarana edutainment dalam pembelajaran biologi. Penggunaan Instagram juga berpotensi untuk meningkatkan motivasi, interaksi, dan kreativitas serta menyenangkan dalam pembelajaran biologi.
Kata kunci: Instagram, edutainment, persepsi, calon guru biologi,
Hubungan Tingkat Pengetahuan dan Perilaku Siswa-Siswi SMA tentang 3 M Dalam Upaya Mencegah Penyebaran COVID-19 di Kabupaten Kepulauan Anambas Tahun 2021
Authors: Martias Indra , Aldy Diaz , Idris Fadhil
Published: March 2022

Dalam upaya memutus mata rantai penyebaran COVID-19 diperlukan tingkat pengetahuan dan perilaku mengenai 3M. 3M adalah tindakan preventif yang dianjurkan oleh Kementerian Kesehatan antara lain : mencuci tangan, Menjaga jarak, dan Memakai masker. Penelitian ini merupakan penelitian kuantitatif dengan metode survei. Instrumen dalam penelitian ini adalah kuesioner online dengan menggunakan google form. Perhitungan besar sampel dilakukan menggunakan rumus survei Lemeshow dengan jumlah populasi sebesar 1.409 siswa/siswi SMA di Kepulauan Anambas. Besar sampel yang diperlukan adalah sebanyak 93 siswa/siswi. Untuk melihat hubungan antara pengetahuan dan perilaku siswa/i dianalisis dengan menggunakan Uji Chi Square. Hasil analisis univariat didapatkan tingkat pengetahuan siswa/i dengan kategori baik sebesar 77 orang (82,8%), cukup 15 orang (16,1%) dan kurang 1 orang (1,1%). sedangkan perilaku siswa/i dengan kategori baik berjumlah 80 orang (86,1%), cukup 11 orang (11,9%) dan kurang 2 orang (2,2%). Analisis Bivariat menunjukkan hubungan yang signifikan antara pengetahuan dengan prilaku siswa/i SMA se Kabupaten Anambas dalam menerapkan 3 M (P value 0,00 < 0,05). Kesimpulan: rata-rata tingkat pengetahuan dan perilaku siswa/i SMA di Kepulauan Anambas dalam kategori baik, pengetahuan berbanding lurus dengan perilaku siswa dalam menerapkan 3 M. Dibutuhkan kesiapan sekolah dalam menghadapi pembelajaran tatap muka sehingga penyebaran virus covid 19 tidak terjadi di sekolah.
Pengetahuan Mengenai Bahaya Merokok dengan Keinginan Berhenti Merokok Masyarakat Desa Pakel
Authors: Iriyanti Yudha Nur , Mandagi Ayik Mirayanti
Published: March 2022

Merokok merupakan kebiasaan yang memiliki dampak buruk bagi kesehatan maupun lingkungan. Prevalensi merokok di Indonesia terus mengalami peningkatan setiap tahunnya, baik pada laki-laki ataupun perempuan. Tujuan dari penelitian ini untuk mengetahui promosi kesehatan mengenai bahaya merokok yang akan menimbulkan keinginan untuk berhenti merokok. Penelitian ini merupakan penelitian deskriptif dengan menggunakan pendekatan kuantitatif. Penelitian ini menggunakan total populasi sebanyak 30 responden masyarakat Desa Pakel. Penelitian ini menggunakan instrumen berupa kuesioner penelitian. Pada usia <15 tahun responden sudah mulai merokok dengan persentase sebesar 86,7%. Sebagian besar keadaan yang membuat merokok pada responden adalah saat santai sebesar 66,7%. Masyarakat memiliki tingkat pengetahuan yang tinggi mengenai dampak dari bahaya merokok dengan persentase sebesar 73,3%. Masyarakat memiliki keinginan yang tinggi untuk berhenti merokok hal ini didapatkan dengan persentase sebesar 86,7%. Kesimpulan dalam penelitian ini tingkat pengetahuan masyarakat yang tinggi mengenai bahaya merokok mendorong keinginan seseorang untuk berhenti merokok
Pengaruh Pengetahuan Dan Sikap Terhadap Kesiapsiagaan Sumber Daya Manusia Puskesmas Batumarmar Dalam Menghadapi Bencana Kekeringan
Authors: Aini Siti Nurul
Published: March 2022

Indonesia merupakan negara yang penuh dengan ancaman bencana, salah satunya ialah bencana kekeringan. Tahun 2018 Kabupaten Pamekasan dinyatakan termasuk dalam daerah dengan indeks resiko bencana cuaca ekstrim berkategori tinggi yakni sebesar 13,6. Dampak jangka panjang yang dapat ditimbulkan dari bencana kekeringan ialah masalah gizi buruk hingga stunting. Wilayah kerja Puskesmas Batumarmar menjadi wilayah dengan desa kering kritis terbanyak di Pamekasan. Kondisi tersebut menuntut SDM Puskesmas Batumarmar untuk meningkatkan kesiapsiagaan dalam menghadapi bencana kekeringan. Penelitian ini bertujuan untuk mengetahui adanya pengaruh pengetahuan dan sikap terhadap kesiapsiagaan SDM Puskesmas dalam menghadapi bencana kekeringan. Penelitian ini menggunakan metode penelitian kuantitatif dengan pendekatan cross sectional. Analisis data pada penelitian ini menggunakan uji Regresi Logistik sederhana yang ditujukan untuk dapat melihat adanya pengaruh antara variabel independen terhadap variabel dependen. Penelitian dilakukan pada SDM Puskesmas Batumarmar Kabupaten Pameksan dengan jumlah sample sebanyak 51 orang. Hasil menunjukkan bahwa, tingkat kesiapsiagaan SDM Puskesmas Batumarmar dalam menghadapi bencana kekeringan dominan masuk dalam kategori belum siap yakni sebesar 78,4%. Tingkat pengetahuan SDM terhadap bencana kekeringan didominasi buruk yakni sebesar 82,4%. Termasuk hasil sikap terhadap kesiapsiagaan bencana kekeringan yang lebih didominasi oleh sikap negative sebesar 82,4%. Kesimpulan dari penelitian ini ialah, terdapat pengaruh antara pengetahuan dan sikap terhadap kesiapsiagaan SDM Puskesmas Batumarmar dalam menghadapi bencana kekeringan.
Gambaran Pelaksanaan Program Pengendalian dan Pemberantasan COVID-19 di Wilayah Kerja Puskesmas Keputih Surabaya 2020
Authors: Lukitaruna Devinafarica , Hendrati Lucia Yovita , K.N Arik
Published: March 2022

Kota Surabaya sebagai Ibukota Provinsi Jawa Timur menjadi penyumbang angka terbesar kasus COVID-19 di Jawa Timur. Penularan virus terjadi sangat cepat di masyarakat. Puskesmas merupakan fasilitas pelayanan kesehatan tingkat pertama yang menjadi perantara antara instansi kesehatan dan masyarakat. Puskesmas Keputih merupakan salah satu Puskesmas dengan angka kasus COVID-19 yang tinggi di Kota Surabaya. Maka dari itu perlu dilakukan penelitian berupa analisis gambaran program pengendalian COVID-19 di wilayah kerja Puskesmas Keputih sebagai Puskesmas dengan angka kasus COVID-19 yang tinggi di Surabaya. Tujuan dari penelitian ini adalah mengidentifikasi dan menganalisis gambaran pelaksanaan program pengendalian dan pemberantasan COVID-19 di wilayah kerja Puskesmas Keputih Kota Surabaya periode bulan Maret – Desember 2020. Metode penelitian ini adalah kuantitatif dengan jenis penelitian deskriptif dan menggunakan desain penelitian cross sectional. Hasil penelitian ini yaitu Puskesmas Keputih melakukan tindakan pengendalian dan pemberantasan COVID-19 menggunakan 5 level pencegahan yaitu promosi kesehatan, perlindungan khusus, deteksi dini dan tindakan tepat, pembatasan kecacatan, dan rehabilitasi. Kesimpulan dari penelitian ini adalah program-program kerja Puskesmas Keputih seluruhnya telah sesuai dengan himbauan dan anjuran dari pemerintah yang disampaikan melalui buku panduan pengendalian COVID-19 yang diterbitkan oleh Kementerian Kesehatan. Selain itu program kerja Puskesmas Keputih juga sejalan dengan beberapa hasil penelitian yang diunggah di jurnal internasional.
Caracterización clínico-epidemiológica del tromboembolismo pulmonar en autopsias del Instituto de Medicina Legal de Medellín 2010-2020
Authors: Pareja Pineda Jorge Iván , Londoño Betancur Sara Vanessa , Cardona Arias Jaiberth Antonio
Published: March 2022

Introducción: A pesar de disponer de métodos diagnósticos y terapéuticos eficaces, el tromboembolismo pulmonar (TEP) es una afección que a lo largo de décadas ha causado una elevada morbilidad y mortalidad.

Objetivo: Describir las características clínico-epidemiológicas y anatomo-patológicas de los diagnósticos de TEP en cadáveres que ingresaron por muerte súbita al Instituto Nacional de Medicina Legal y Ciencias Forenses de Medellín 2010-2020.

Métodos: Estudio descriptivo de una cohorte retrospectiva, formada por los casos que ingresaron al Instituto de Medicina Legal por muerte súbita con causa de muerte por establecer, diagnosticados  con TEP. Los análisis se realizaron con frecuencias y medidas de resumen en SPSS 27.0.

Resultados: Entre 46097 necropsias médico legales realizadas en el período estudiado, 164 casos (0,36 %) corresponden a TEP. La edad media fue 57,0±19,3 años y 51,2% mujeres. El 57,9% fue muerte natural, en 37,2% se presentó trauma y 36,0% otro evento circulatorio. En la mayoría de los casos, el deceso ocurrió en la vivienda (47,0%) y la vía pública (25,6%). En la trombosis, 45,1% fue de la arteria pulmonar (trombo cabalgado); 34,8% de arterias pulmonares intraparenquimatosas y 52,4% de los fallecidos presentaron trombosis venosa profunda de miembros inferiores.

Conclusión: El TEP es una importante causa de muerte en pacientes intra y extrahospitalarios, a pesar de los métodos diagnósticos, farmacológicos y no farmacológicos existentes para su prevención. La realización de un mayor número de autopsias clínicas permitirá revelar el verdadero impacto en nuestro medio de esta complicación y mejorar la calidad en el cuidado de los pacientes.
Excessive phytochelatin and metallothione enhance reactive oxygen species generation in absence of heavy metal
Authors: Huda A.K.M. Nazmul , Hossain Muktar , Mukta Rumana Haque , Khatun Mossammad Rima , Haque Md. Anwarul
Published: March 2022

Ethylene diamine tetraacetic acid (EDTA) is a chelating agent used
worldwide for the phytoextraction of heavy metals as well as household
and industrial purposes and persists in the cultivated land for a long
time. In this study, the effect of EDTA in the presence and in absence
of heavy metal (Chromium) was evaluated on non-hyper accumulating plant
rice (Oryza sativa L.) on the hydroponic solution at the cellular and
molecular level. EDTA application enhanced phytochelatin (PC) and
metallothionein (MT) synthesis, which was assured by up regulation of
phytochelatin and metallothionein gene in rice root. However, lower
quantity of EDTA (25µM) along with chromium enhanced the chromium (Cr)
uptake in root but inhibited its translocation to shoot pointing
vacuolar sequestration of excessive chromium in root. One the other
hand, in absence of chromium, H2O2 concentration is found to be
increased which significantly enhanced electrolyte leakage and lipid
peroxidation compared with control plants. The findings of this
investigation exhibited that excessive PCs and MTs owing to EDTA enhance
reactive oxygen species (ROS) generation in absence of heavy metal
(Chromium).
Rapid differential detection of H1 and H3 subtype swine influenza viruses by a TaqMan-MGB based duplex one-step real time RT-PCR assay
Authors: Chen Kaibiao , Kong Ming , Liu Jiao , Jiao Jun , Zeng Zixiong , Bu Xinxin , Yan Yayao , Chen Yu , Gao Ruyi , Liu Xiaowen , Wang Xiaoquan , Hu Jiao , Hu Shunlin , Jiao Xinan , Gu Min , Liu Xiufan
Published: March 2022

Background: Swine influenza is not only an economically important
respiratory disease in swine, but also constantly poses a threat to
human health. Hence, developing a rapid, sensitive and efficient
detection method of swine influenza virus (SIV) is highly essential.
Method: By aligning the HA gene sequences of SIV circulating in China in
recent 10 years, a H1 primer-probe set targeting both Eurasian
avian-like H1N1 (EA H1N1) and Pandemic 2009 H1N1 (Pdm09 H1N1) lineages
plus a H3 prime-probe set targeting the prevalent human-like H3N2 (HL
H3N2) subtype were designed, respectively. Further, a TaqMan-MGB based
duplex one-step real time RT-PCR (RRT-PCR) assay was established, and
the sensitivity, specificity and repeatability were evaluated. Results:
The duplex RRT-PCR exhibited good sensitivity with the detection limit
of 5 copies/μL HA plasmid for each of the EA H1N1, Pdm09 H1N1 and HL
H3N2 subtype SIVs, and matched a detection accuracy of 94.4% (17/18)
with traditional virus isolation through chicken embryo inoculation
using experimentally infected mice lung samples. Besides, the method
showed high repeatability both within-run and between-runs, and no
cross-reactivity against some commonly circulated porcine viruses in
China. Furthermore, the duplex RRT-PCR method revealed a relatively
higher prevalent rate of H1 than H3 subtype SIV in 166 nasal swabs from
pigs in some slaughterhouse during October~December,
2019. Conclusions: This developed assay could be very helpful for rapid
differential detection and routine surveillance of EA H1N1, Pdm H1N1 and
HL H3N2 subtype SIVs in China.
A GIANT CONGENITAL LEFT ATRIAL APPENDAGE ANEURYSM : A RARE ANOMALY
Authors: Zor Ayşegül Karahan , Beşikçi Resmiye , Arslan Ahmet , Aydıner Ömer , Çiçek Sertaç
Published: March 2022

Left atrial appendage aneurysm (LAAA) is a rare congenital anomaly which
can be associated with cardiovascular morbidity and mortality by
predisposing to atrial tachyarrhythmias, thromboembolism and cardiac
failure. In this case report a patient presenting with supraventricular
arrythmias and chest pain due to LAAA is presented. Surgical treatment
was performed through median sternotomy with cardiopulmonary bypass
through which the giant aneurysm of the left atrial appendage, extending
to the apex of the left ventricle was surgically managed successfully.
Surgical treatment is curative and proposed as the main treatment
modality regardless of the presence or absence of symptoms.
Subsurface soil carbon and nitrogen losses offset surface accumulation in abandoned agricultural fields
Authors: Yang Yi , Knops Johannes
Published: March 2022

Abandoned agricultural fields are thought to accumulate soil organic
matter after cultivation cessation. Most research on soil C and N
sequestration has focused on the surface and overlooked their dynamics
below 30 cm. With 13-year long-term surveys of 21 old field, we found
soil C and N accumulated by 30.5 ± 6.5 g C m⁻² yr⁻¹ and 2.41 ± 0.49 g N
m⁻² yr⁻¹ in the surface (0-20 cm) yet decreased by 64.6 ± 12.5 g C m⁻²
yr⁻¹ and 6.83 ± 1.34 g N m⁻² yr⁻¹ in the subsurface (20-100 cm). Such C
and N losses in subsurface soil can be attributed to the shallow root
distribution, which is likely caused by the continuing dominance of
non-native and shallow rooted C3 grasses and the lack of legumes after
abandonment. Thus, vegetation changes are a legacy effect of agriculture
that leads to ongoing soil C losses in abandoned agricultural fields.
Blocking IgE with L-glutamic acid analogs as an alternative approach to allergy treatment
Authors: Campos-Mesquita Debora , Souza Giliane , Carrera Marinete , Giraldi-Guimarães Arthur , Machado Olga
Published: March 2022

Background: IgE–mediated allergic diseases have increased in the last
decades. The most prevalent allergens from castor seeds are Ric c1 and
Ric c3, isoforms of 2S albumin. These allergenic proteins cross-react
with allergens from peanut, shrimp, fish, corn, gramineous, house dust,
and tobacco. The usual allergy treatment employs antihistaminic and,
immunotherapies. We propose an alternative immunotherapeutic approach,
denoted “IgE-blockage” by L-Glutamic acid or modified-glutamic acid.
Methods: Six compounds, D-glutamic acid, L-glutamic acid,
N-methyl-L-glutamic acid, N-acetyl-L-glutamic acid, N- (4-nitrobenzoyl)
-L-glutamic acid, and N-carbamyl-L-glutamic, were tested as a blocker.
To evaluate motor coordination and the sedative/hypnotic activity of
L-glutamic acid, a rota-rod test and a thiopental sodium-induced
sleeping test were used. Results: the compounds, L- Glutamic acid and
L-Nitrobenzoyl glutamic acid, were the most active compounds to block
the interaction of castor allergens with IgE. These compounds also
prevent cross-responses with allergens from food sources and inhalants
that cross-react with them. In the sleeping test, the groups that
received L-glutamic acid at doses of 10 and 30 mg/kg had a sleeping time
similar to the vehicle control group. No changes in the animals’
behavior were observed and there was no difference between the
L-glutamic acid groups and the vehicle control groups in the rota-rod
test. Conclusion: L-glutamic acid and L-Nitrobenzoyl glutamic acid could
be used as IgE blockers to prevent allergic diseases.
Proteomic profile of extracellular vesicles in anaphylaxis and their role in vascular permeability
Authors: Nuñez-Borque Emilio , Fernandez-Bravo Sergio , Pastor-Vargas Carlos , Alvarez-Llama Gloria , Gutierrez-Blazquez Maria , Alwashali Ebrahim , Laguna José , Dionicio Javier , Betancor Diana , Villalobos Victoria , Tome-Amat Jaime , Herranz Javier Cuesta , Benito-Martin Alberto , Esteban Vanesa
Published: March 2022

Background: Anaphylaxis is the most severe manifestation of allergic
disorders. Diagnostic and understanding of molecular mechanisms need to
improve. Extracellular vesicles (EVs) play a key role in cellular
communication offering new possibilities to unravel patient-based
particularities. The aim of this study is to analyze the protein profile
of anaphylaxis-derived EVs providing a resource of potential markers for
anaphylactic reactions, and to characterize their molecular mechanisms.
Methods: EVs were isolated from 86 plasma samples (collected from 43
patients) during the acute phase of anaphylaxis (AnEVs) and at their
baseline (BEVs). For comparison, EVs were characterized and their
protein patterns were analyzed by mass spectrometry-based quantitative
proteomics (LC-MS/MS). System Biology Analysis (SBA) was applied to
identify main canonical pathways and molecules involved. In addition, in
vitro permeability assays based on EVs-endothelial cells (ECs) were
performed. Results: Differential proteomic analysis performed in 10 EVs
paired patients’ samples identified 1206 proteins of which 99 were
modulated in the AnEVs signature. CDC42, Ficolin-2 and S100A9 enrichment
was confirmed in a larger cohort of patients. SBA revealed diverse group
of immune proteins as the main canonical pathways altered in AnEVs.
Thus, leukocyte extravasation and granulocyte adhesion-diapedesis
processes stand out. In addition, marked-EVs from anaphylactic patients
were captured by ECs decreasing the resistance of human endothelial
monolayers. Conclusion: Our findings identify for the first time a
differential EVs pattern signature in anaphylaxis revealing a source of
potential biomarkers. Furthermore, these vesicles could participate in
altered immune molecular mechanisms and present a role increasing
vascular permeability.
Distinct roles of PI3Kδ and PI3Kγ in a toluene diisocyanate-induced steroid insensitive murine asthma model
Authors: Xu Caiyun , Chen Shuyu , Deng Yao , Song Jiafu , Li Jiahui , Chen Xin , Chang Ping , Yao Lihong , Tang Haixiong
Published: March 2022

Background: TDI-induced asthma is characterized by neutrophil-dominated
airway inflammation and often associated with poor responsiveness to
steroid treatment. Both PI3Kδ and PI3Kγ have been demonstrated to play
important proinflammatory roles in ovalbumin-induced asthma. We’ve
already reported that blocking pan PI3K effectively attenuated
TDI-induced allergic airway inflammation. Yet the specific functions of
PI3Kδ and PI3Kγ in TDI-induced asthma are still unclear. Methods: Male
BALB/c mice were first dermally sensitized and then challenged with TDI
to generate an asthma model. Sellective inhibitors of PI3Kδ (IC-87114,
AMG319) and PI3Kγ (AS252424, AS605240) as well as prednisone were
respectively given to the mice after each airway challenge. Results:
Treatment with IC-87114 or AMG319 after TDI exposure led to
significantly decreased airway hyperresponsiveness (AHR), less
neutrophil and eosinophil accumulation, attenuated airway smooth muscle
(ASM) thickening, less M1 and M2 macrophages in lung, as well as lower
levels of IL-4, IL-5, IL-6 and IL-18 in bronchoalveolar lavage fluid
(BALF) and recovered IL-10 production. While mice treated with AS252424
or AS605240 had increased AHR, more severe ASM thickening, larger
numbers of neutrophils and eosinophils, more M1 but less M2 macrophages,
and higher BALF levels of IL-4, IL-5, IL-6, IL-10, IL-12, IL-18 when
compared with those treated with vehicle. Conclusion: These data
revealed that pharmacological inhibition of PI3Kδ attenuates TDI-induced
steroid insensitive airway inflammation while PI3Kγ inhibition
exacerbates TDI-induced asthma, indicating distinct biological functions
of PI3Kδ and PI3Kγ in TDI-induced asthma.
Real-world experience of selexipag titration in pulmonary arterial hypertension
Authors: Cullivan Sarah , McCormack Ciara , Natarajan Anandan , Gaine Sean , McCullagh Brian
Published: March 2022

Selexipag is an oral selective prostacyclin receptor agonist, that was
approved for use in patients with NYHA functional class II-III pulmonary
arterial hypertension (PAH). In the GRIPHON study, selexipag
demonstrated consistent efficacy for individualised doses in low, medium
and high dose stratums. In order to better understand the real world
approach to selexipag titration and to establish the individualised
maintenance regimens used in our centre, we performed this retrospective
study of the first 20 patients prescribed selexipag. Baseline
characteristics differed from the GRIPHON study, with more combination
therapy and comorbidities at baseline; however. Maintenance doses were
stratified as low-dose in 10% (n=2), medium-dose in 70% (n=14) and
high-dose in 20% (n=4). Furthermore, two of these patients were
successfully transitioned from inhaled iloprost. This study demonstrates
that selexipag can be safely initiated, titrated and transitioned in an
outpatient setting to achieve an individualised dosing regimen.
Maternal Fear of COVID-19 and prevalence of postnatal depression symptoms: Risk and protective factors
Authors: Gluska Hadar , Shiffman Noga , Mayer Yael , Elyasyan Lior , Elia Nofar , Daher Rawan , Sharon-Weiner Maya , Miremberg Hadas , Kovo Michal , Biron-Shental Tal , Gabbay-Benziv Rinat
Published: March 2022

Objective: To evaluate the relations between Fear of COVID-19 and
postpartum depression (PPD) symptoms. Design: A multicenter prospective
observational study. Setting and Population: A cohort of women who
delivered during COVID-19 pandemic between 03-05/2020. Methods:
Participants were virtually approached after delivery and asked to
complete an online questionnaire. Data was verified with each center’s
perinatal database. The validated Fear of COVID-19 Scale was in use. PPD
was evaluated using the EPDS questionnaire as a categorical (≥10) and as
a continuous scale. Pre-existing maternal disability was defined as any
prior physiological/psychological chronic health condition.
Stress-contributing complications during pregnancy or at birth included
pregnancy and labor related complications. Regression analysis and ROC
statistics were utilized to evaluate associations and control for
confounders. Main Outcome Measure: PPD symptoms. Results: Overall, 421
women completed the questionnaires. Of them, 99(23.5%) had a high EPDS
score. Fear of COVID-19 was positively correlated with PPD symptoms
(r=0.35,p=0.000),ROC-AUC 0.67, 95%CI 0.61-0.74. Following adjustment to
confounders (maternal age, nulliparity, ethnicity, marital status,
financial difficulties, maternal disability, accessibility to medical
services, and stress-contributing complications during pregnancy (, the
most important factor that correlated with depression was maternal
disability (aOR3,95%CI 1.3-6.9) followed by Fear of COVID-19
(aOR1.1,95%CI 1.05-1.15). High accessibility to medical services
(aOR0.59,95% CI 0.45-0.77) and stress-contributing complications during
pregnancy (aOR0.2, 95% CI 0.11-0.82) were both protective for PPD
symptoms. Conclusions: During the COVID-19 pandemic, maternal disability
and Fear of COVID-19 are positively associated with a high EPDS score.
High medical accessibility was found as a protective factor for PPD.
Heat wave event facilitates defensive responses in invasive C3 plant under elevated CO2 concentration to the detriment of insect herbivores
Authors: Tian Zhenya , Ma Chao , Zhao Chenchen , Zhang Yang , Gao Xuyuan , Tian Zhenqi , Chen Hongsong , Guo Jianying , Zhou Zhongshi
Published: March 2022

To predict and mitigate the effects of climate change on communities and
ecosystems, the joint effects ofextreme climatic events on species
interactions need to be understood.Using the common ragweed (Ambrosia
artemisiifolia L.)—leaf beetle (Ophraella communa) system, we
investigated the effects of heat wave and elevated CO2 on common ragweed
growth, secondary metabolism, and the consequent impacts on the beetle.
The results showed that elevated CO2 and heat wave facilitated A.
Artemisiifolia growth; further, A. artemisiifolia accumulated large
amounts of defensive secondary metabolites. Being fed on A.
artemisiifolia grown under elevated CO2 and heat wave conditions
resulted in the poor performance of O. communa (high mortality, long
development period, low reproduction). Overall, under elevated CO2, heat
wave strengthened the defensive ability of A. artemisiifolia to
herbivores. Super adaptability to climatic changes may aggravate
invasive plant distribution, posing a challenge to the control of
invasive plants in the future.
A NOVEL GENETIC ALGORITHM APPROACH TO THE MAXIMUM INDEPENDENT SET PROBLEM
Authors: Gencer Mehmet , BERBERLER MURAT
Published: March 2022

A NOVEL GENETIC ALGORITHM APPROACH TO THE MAXIMUM INDEPENDENT SET
PROBLEM
Long-acting opioids and cardiovascular diseases: Help or hindrance!
Authors: Mehta Ankita , Patel Bhoomika
Published: March 2022

Opioids are widely being used for chronic pain management, cough and
diarrhea suppressants, anesthetic agents, and opioid de-addiction
therapy. Opioid receptors, present in the central nervous system and
peripheral tissues, are documented to regulate several cardiac functions
through different signaling pathways. Long-acting opioids (LAO) have
been successfully evaluated for their beneficial effects in various
cardiovascular diseases viz. myocardial infarction, ischemic reperfusion
injuries, atherosclerosis etc. However, on the other hand, several
research studies pointed towards the harmful effects of LAOs which are
mainly associated with QTc prolongation, torsade de pointers,
ventricular arrhythmias, and cardiac arrest. This review shall
familiarize readers with the benefits as well as the harmful effects of
long-acting opioids in cardiovascular diseases. We have also provided an
overview of cardiac opioid receptors, endogenous cardiac opioid
peptides, and regulation of cardiovascular functions by central and
cardiac opioid receptors.
Monotonicity and Symmetry of Positive Solution for 1-Laplace Equation
Authors: Zhao Lin
Published: March 2022

In this paper we deal with a Dirichlet problem for an elliptic equation
involving the 1-Laplace operator. Under suitable assumptions on the
nonlinearity we show that there exists a symmetrical, monotonic and
positive solution via the moving plane method. We show the a priori
estimates for the positive solution.
Clinical study on the treatment of pelvic organ prolapse by sacrospinous ligament fixation
Authors: Xia Jingxian , yang jian , Wang Wenyan , Wei Bing , Fu Liutao , Zhang Lili , Song Enxue , Li Congqing
Published: March 2022

Objective To study clinical efficacy and quality of life of sacrospinous
ligament fixation in the treatment of pelvic organ prolapse. Design
Retrospective analysis of clinical cases Setting China Population or
sample 124 cases of patients with pelvic organ prolapse of The second
affiliated hospital of anhui medical university were retrospectively
analyzed from January 2013 to December 2019. Methods Perioperative
information, postoperative complications, postoperative subjective
recovery and postoperative quality of life questionnaire were observed
and compared. According to different postoperative follow-up time,
patients were divided into the <12 months group, the 12-24
months group, the 24-60 months group and the ≥60 months group, and the
subjective postoperative recovery of patients in different groups was
counted respectively. Main outcome measures POP-Q stages were in normal
position after 3 months of postoperation, and the values of genital
hole, perineal body, and total vaginal length were significantly
different from those before operation (P<0.001). The results
of the pelvic floor dysfunction questionnaire for 19 patients after 12
months of postoperation showed that the total PFDI-20 score was
significantly different from that before surgery (P<0.001),
the preoperative and postoperative scores of UDI-6(urogenital distress
inventory) and POPDI-6(pelvic organ prolapse distress inventory) were
significantly different (P<0.001). Preoperative and
postoperative hemoglobin difference was statistically significant (P
<0.001), while preoperative and postoperative residual urine
values were not statistically significant (P >0.05).
Conclusion Sacrospinous ligament fixation (SSLF) has a significant
effect on the treatment of moderate and severe pelvic organs prolapse,
and can effectively restore its anatomical structure.
NLRP3 Inflammasome: Mechanism of Action, Role, and Therapeutics in Liver Diseases
Authors: Ye Wen-Chu , Huang Shi-Feng , Hou Lian-Jie , Jiang Ting , Lin Shu-Yun , Hu Ching Yuan , Zhao Guo-Jun
Published: March 2022

Liver inflammation is a universal characteristic of chronic liver
diseases. NLRP3 is an intracellular sensor that recognizes various
endogenous danger signals and environmental irritants, contributing to
the formation and activation of the NLRP3 inflammasome. NLRP3
inflammasome is closely related to the progression of various liver
diseases and is strongly associated with replicating COVID-19, which is
still spreading globally. The assembly and activation of NLRP3
inflammasome in the liver diseases aggravate inflammation and subsequent
fibrosis, and this effect is abolished by genetic or pharmacologic
deletion of NLRP3 inflammasome. Here, we summarized the latest advances
in the critical regulatory role of NLRP3 inflammasome in a variety of
liver diseases, including COVID-19 induced liver diseases, NAFLD, ALD,
and ischemia-reperfusion (I/R) injury. Additionally, we also discuss
small-molecule inhibitors identifying the NLRP3 inflammasome signaling
are novel therapeutic targets in treating liver diseases. Our review
provides novel insights into the underlying mechanisms of NLRP3
inflammasome in liver diseases and may offer a potential therapeutic
strategy for treating liver diseases by targeting NLRP3 inflammasome.
Hybrid Numbers with Fibonacci and Lucas Hybrid Number Coefficients
Authors: Polatlı Emrah
Published: March 2022

In this paper, we introduce hybrid numbers with Fibonacci and Lucas
hybrid number coefficients. We give the Binet formulas, generating
functions, exponential generating functions for these numbers. Then we
define an associate matrix for these numbers. In addition, using this
matrix, we present two different versions of Cassini identitiy of these
numbers.