bluuebunny/crossref_metadata_2025 huggingface dataset:
COWPEA (VIGNA UNGUICULATA) CROPS IN AFRICA CAN RESPOND TO INOCULATION WITH RHIZOBIUM
Authors: BODDEY ROBERT M. , FOSU MATHIAS , ATAKORA WILLIAMS K. , MIRANDA CESAR H. B. , BODDEY LUCIA H. , GUIMARAES ANA PAULA , AHIABOR BENJAMIN D. K.
Published: October 2016

Cowpea (Vigna unguiculata L. Walp) is the most important food grain legume in Africa. Cowpea is nodulated by rhizobium bacteria in almost all soils of the tropics, but studies performed in the 1970s and 1980s in Nigeria suggested only modest responses of grain yield in the field to inoculation of selected rhizobium strains. More recently, experiments performed in Brazil have shown that cowpea responded to inoculation of rhizobium selected locally and grain yields increased by up to 30%. We tested some of the Brazilian strains on cowpea at a site in northern Mozambique and at several sites in Northern Ghana. At all sites phosphorus fertilizer (26 kg P ha−1) was added to all plots. At the site in Mozambique despite considerable damage to the crop by the parasitic yellow witchweed (Alectra vogelii), grain yields were more than doubled by inoculation of one of the Brazilian strains and reached 1.4 Mg ha−1. In on-station experiments conducted in 2012 in June and August in northern Ghana using the local cowpea variety Padi-Tuya as the test crop, nodule weight at 35 days after planting (dap) tripled with rhizobium strain BR 3299 (530 mg plant−1) in August with the other inoculants (BR 3267 and a mixture of BR 3267 and BR 3299) also increased nodule weight to over 300 mg plant−1. In the first on-station experiment, grain yields were doubled by the inoculation of any of the three rhizobium strains, and in the second experiment, significant increases in grain yield ranged from 39% to 57% and reached over 2.0 Mg ha−1. Similar increases in nodulation and grain yield due to inoculation were observed in 22 on-farm trials. Nitrogen fertilizer application promoted vegetative growth but did not increase grain yield and nodulation. Inoculating cowpea with highly effective rhizobium strains can therefore enhance grain yield of smallholder farmers in Africa.
PERCEPTIONS OF STIGMA AND DISCRIMINATION IN HEALTH CARE SETTINGS TOWARDS SUB-SAHARAN AFRICAN MIGRANT WOMEN LIVING WITH HIV/AIDS IN BELGIUM: A QUALITATIVE STUDY
Authors: Arrey Agnes Ebotabe , Bilsen Johan , Lacor Patrick , Deschepper Reginald
Published: October 2016

Stigma and discrimination within health care settings remain a public health challenge across diverse cultural environments and may have deleterious effects on mental and physical health. This study explores the causes, forms and consequences of HIV-related stigma and discrimination among migrant sub-Saharan African women living with HIV in Belgium. A qualitative study was conducted with 44 HIV-positive sub-Saharan African migrant women between April 2013 and December 2014 in health care settings in Belgium. Data were analysed using thematic content analysis. Twenty-five of the women reported overt stigma and discrimination and fifteen reported witnessing behaviours that they perceived to be stigmatizing and discriminatory in health care settings. The themes that emerged as to the causes of stigma and discrimination were: public perceptions of migrants and HIV, fear of contamination and institutional policies on HIV management. Reported forms of stigma and discrimination included: delayed or denied care, excessive precautions, blame and humiliation. The consequences of stigma and discrimination were: emotional stress, inconsistent health-care-seeking behaviour and non-disclosure to non-HIV treating personnel. Stigma and discrimination in health care settings towards people with HIV, and more specifically towards HIV-positive sub-Saharan African migrant women, impedes sustainable preventive measures. Specialized education and training programmes for non-HIV health care providers require in-depth investigation.
MATERNAL HEIGHT AND PRE-PREGNANCY WEIGHT STATUS ARE ASSOCIATED WITH FETAL GROWTH PATTERNS AND NEWBORN SIZE
Authors: Pölzlberger Eva , Hartmann Beda , Hafner Erich , Stümpflein Ingrid , Kirchengast Sylvia
Published: October 2016

The impact of maternal height, pre-pregnancy weight status and gestational weight gain on fetal growth patterns and newborn size was analysed using a dataset of 4261 singleton term births taking place at the Viennese Danube Hospital between 2005 and 2013. Fetal growth patterns were reconstructed from three ultrasound examinations carried out at the 11th/12th, 20th/21th and 32th/33th weeks of gestation. Crown–rump length, biparietal diameter, fronto-occipital diameter, head circumference, abdominal transverse diameter, abdominal anterior–posterior diameter, abdominal circumference and femur length were determined. Birth weight, birth length and head circumference were measured immediately after birth. The vast majority of newborns were of normal weight, i.e. between 2500 and 4000 g. Maternal height showed a just-significant but weak positive association (r=0.03: p=0.039) with crown–rump length at the first trimester and with the majority of fetal parameters at the second trimester (r>0.06; p<0.001) and third trimester (r>0.09; p<0.001). Pre-pregnancy weight status was significantly positively associated with nearly all fetal dimensions at the third trimester (r>0.08; p<0.001). Maternal height (r>0.17; p<0.001) and pre-pregnancy weight status (r>0.13; p<0.001), but also gestational weight gain (r>0.13; p<0.001), were significantly positively associated with newborn size. Some of these associations were quite weak and the statistical significance was mainly due to the large sample size. The association patterns between maternal height and pre-pregnancy weight status with fetal growth patterns (p<0.001), as well as newborn size (p<0.001), were independent of maternal age, nicotine consumption and fetal sex. In general, taller and heavier women gave birth to larger infants. This association between maternal size and fetal growth patterns was detectable from the first trimester onwards.
Acetoacetic acid induces oxidative stress to inhibit the assembly of very low density lipoprotein in bovine hepatocytes
Authors: Shi Xiaoxia , Li Dangdang , Deng Qinghua , Peng Zhicheng , Zhao Chenxu , Li Xiaobing , Wang Zhe , Li Xinwei , Liu Guowen
Published: October 2016

Dairy cows with fatty liver or ketosis exhibit hyperketonemia, oxidative stress, and a low rate of very low density lipoprotein (VLDL) assembly, and there may be a potential link among these characteristics. Therefore, the objective of this study was to determine the effect of acetoacetic acid (AcAc) on the assembly of VLDL in cow hepatocytes. Cultured cow hepatocytes were treated with different concentrations of AcAc with or without N-acetylcysteine (NAC, an antioxidant). AcAc treatment decreased the mRNA expression and activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), and significantly increased malondialdehyde (MDA) content, indicative of oxidative stress. Furthermore, AcAc treatment significantly down-regulated the mRNA expression of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), and low density lipoprotein receptor (LDLR), which thus decreased VLDL assembly and increased triglyceride (TG) accumulation in these bovine hepatocytes. Importantly, NAC relieved AcAc-induced oxidative stress and increased VLDL assembly. In summary, these results suggest that AcAc-induced oxidative stress affects the assembly of VLDL, which increases TG accumulation in bovine hepatocytes.
Variation of milk coagulation properties, cheese yield, and nutrients recovery in curd of cows of different breeds before, during and after transhumance to highland summer pastures
Authors: Zendri Francesco , Ramanzin Maurizio , Cipolat-Gotet Claudio , Sturaro Enrico
Published: October 2016

This paper aimed at evaluating the effect of summer transhumance to mountain pastures of dairy cows of different breeds on cheese-making ability of milk. Data were from 649 dairy cows of specialized (Holstein Friesian and Brown Swiss) dual purpose (Simmental) and local (mostly Rendena and Alpine Grey) breeds. The Fourier-Transform Infra-Red Spectra (FTIRS) of their milk samples were collected before and after transhumance in 109 permanent dairy farms, and during transhumance in 14 summer farms (with multi-breeds herds) of the Trento Province, north-eastern Italy. A variety of 18 traits describing milk coagulation, curd firming, cheese yield and nutrients recovery in curd/loss in whey were predicted on the basis of FTIRS collected at the individual cow level. Moving the cows to summer farms improved curd firming traits but reduced cheese yields because of an increase of water and fat lost in the whey. During summer grazing, most of cheese-making traits improved, often non-linearly. The milk from summer farms supplementing cows with more concentrates showed better curd firming and cheese yield, because of lower fat lost in the whey. The breed of cows affected almost all the traits with a worst cheese-making ability for milk samples of Holsteins through all the trial, and interacted with concentrate supplementation because increasing compound feed tended to improve cheese-making traits for all breed, with the exception of local breeds for coagulation time and of Brown Swiss for curd firming time. In general, summer transhumance caused a favourable effect on cheese-making aptitude of milk, even though with some difference according to parity, initial days in milk, breed and concentrate supplementation of cows.
Effects of anthropogenic noise on the acoustic behaviour ofSotalia guianensis(Van Bénéden, 1864) in Pipa, North-eastern Brazil
Authors: Leão Martins Dalila T. , Rossi-Santos Marcos R. , Lima Silva Flávio José De
Published: October 2016

This study investigated the emission of subaquatic noise from recreational tourism motorboats, schooners and a sea-bottom mounted water pump. Analyses demonstrated alterations in several whistle (IF:t = 2.42,P = 0.015; FF:t = −2.22,P = 0.025) and calls patterns (MIF:t = −3.13,P = 0.001; MAF:t = −3.49,P = 0.0005; FD:t = −2.21,P = 0.027; D:t = 2.89,P = 0.004), caused primarily by motorboats. Duration of clicks was also modified (D:t = −3.85,P = 0.0001), mainly by the water pump. The frequency range of all noises (0.43–35.8 kHz) overlaps that used by dolphins (1–48 kHz), causing sound emissions changes, with a considerable increase in number of whistles and a reduction in clicks trains. These changes may be a strategy developed by these dolphins to overcome the noise band. Mitigation measures, such as boating regulations and environmental education for the local community, boaters and tourists are needed to conserve the species. The Guiana dolphin population is apparently already suffering, evidenced by diminished residence time and reduced number of individuals entering the inlet during the presence of pleasure craft.
QUALITY OF HEALTH TECHNOLOGY ASSESSMENT REPORTS PREPARED FOR THE MEDICAL SERVICES ADVISORY COMMITTEE
Authors: Hua Martin , Boonstra Tristan , Kelly Patrick J. , Wilson Andrew , Craig Jonathan C. , Webster Angela C.
Published: October 2016

Objectives:The Medical Services Advisory Committee (MSAC) makes recommendations to the Australian Government for funding health technologies under the Medicare Benefits Schedule (MBS). Differences in public, clinical, commercial, and political opinions on health expenditure emphasize the importance of defensible funding decisions. We aimed to evaluate the quality of health technology assessment (HTA) reports over time and among health technologies assessed for MSAC.

Main Outcome Measures:A cohort study was performed of HTA reports prepared for MSAC between 1998 and 2013. We measured the quality of HTA reports using reporting guidelines proposed by the European Collaboration for Assessment of Health Interventions. Individual component scores across eleven domains were calculated, and summed for an overall aggregate score. We used linear regression to investigate any change in quality over time and among the types of technologies assessed.

Results:We included 110 HTA reports. The safety (80 percent), effectiveness (84 percent), economic (74 percent), and organizational (99 percent) domains were better reported than the psychological, social, and ethical considerations (34 percent). The basic (75 percent), methodological (62 percent), background (82 percent), contextual (46 percent), status quo (54 percent), and technical information (66 percent) that framed each assessment were inconsistently reported. On average, overall quality scores increased by 2 percent (p< 0.001) per year, from approximately 60 percent to 80 percent over the 15-year period, with no significant difference among surgical, diagnostic or other nonpharmaceutical health technologies (p= 0.22).

Conclusions:HTA reports prepared for MSAC are a key tool in allocating scarce health resources. The overall quality of these reports has improved, but the reporting of specific domains and subthemes therein could be better addressed.
ASYMPTOTIC SIZE OF KLEIBERGEN’S LM AND CONDITIONAL LR TESTS FOR MOMENT CONDITION MODELS
Authors: Andrews Donald W.K. , Guggenberger Patrik
Published: October 2016

An influential paper by Kleibergen (2005,Econometrica73, 1103–1123) introduces Lagrange multiplier (LM) and conditional likelihood ratio-like (CLR) tests for nonlinear moment condition models. These procedures aim to have good size performance even when the parameters are unidentified or poorly identified. However, the asymptotic size and similarity (in a uniform sense) of these procedures have not been determined in the literature. This paper does so.

This paper shows that the LM test has correct asymptotic size and is asymptotically similar for a suitably chosen parameter space of null distributions. It shows that the CLR tests also have these properties when the dimensionpof the unknown parameterθequals 1. Whenp≥ 2, however, the asymptotic size properties are found to depend on how the conditioning statistic, upon which the CLR tests depend, is weighted. Two weighting methods have been suggested in the literature. The paper shows that the CLR tests are guaranteed to have correct asymptotic size whenp≥ 2 when the weighting is based on an estimator of the variance of the sample moments, i.e., moment-variance weighting, combined with the Robin and Smith (2000,Econometric Theory16, 151–175) rank statistic. The paper also determines a formula for the asymptotic size of the CLR test when the weighting is based on an estimator of the variance of the sample Jacobian. However, the results of the paper do not guarantee correct asymptotic size whenp≥ 2 with the Jacobian-variance weighting, combined with the Robin and Smith (2000,Econometric Theory16, 151–175) rank statistic, because two key sample quantities are not necessarily asymptotically independent under some identification scenarios.

Analogous results for confidence sets are provided. Even for the special case of a linear instrumental variable regression model with two or more right-hand side endogenous variables, the results of the paper are new to the literature.
Determinants of Engagement in Leisure-time Physical Activity – Dialogue with Senior Athletes
Authors: Yamada Naoko , Heo Jinmoo
Published: October 2016

Cet article explore, en examinant leurs croyances saillants, les facteurs qui déterminent si les personnes âgées participent aux Jeux des aînés et de l’activité physique durant les loisirs connexes. Nous avons mené 10 entrevues en profondeur avec des personnes âgées qui ont participé aux Jeux des aînés. Nous avons exploré trois types de croyance sous-tendus par le cadre théorique de comportement prévu: les avantages et les inconvénients (croyances comportementales), le soutien social et la pression (croyances normatives), et les facilitateurs et les obstacles (croyances de contrôle). Les répondants aux entrevues se sont trouvés à s’engager aux Jeux des aînés et à l’activité physique dans la mesure où ils ont associé divers avantages intangibles avec les Jeux et en ont valorisé la satisfaction psychologique. Ils considéraient leurs pairs et les familles comme soutenant et approuvant leur engagement, tout en reconnaissant les capacités physiques et les contraintes structurelles nécessaires pour se livrer aux Jeux et les activités connexes. Les résultats indiquent comment les croyances pertinentes peuvent être combinées avec des interventions pour encourager pour les personnes âgées des activités physiques des loisirs.
Hydrography and dynamics of Port Foster, Deception Island, Antarctica
Authors: Flexas Mar M. , Arias Mariano R. , Ojeda Miguel A.
Published: October 2016

The circulation and water masses of Port Foster, Deception Island, were studied using conductivity-temperature-depth stations inside and outside the semi-enclosed bay and an array of bottom temperature sensors moored around the perimeter of the bay over two weeks in the summer of 2012. Inside Port Foster, the water column is divided into two layers separated by a temperature-forced, seasonal pycnocline at ~40–60 m. The circulation of the upper layer is in an anticlockwise direction, with mean geostrophic currents of ~0.04–0.10 ms-1. The lower layer, from ~60 m to the seabed, shows coastal-trapped waves travelling in a clockwise direction, possibly triggered by local wind gusts. Local sea ice melt in areas surrounding the underwater hot springs of Pendulum Cove appears as a fresh, warm anomaly down to 30 m.
Childhood adversity and epigenetic regulation of glucocorticoid signaling genes: Associations in children and adults
Authors: Tyrka Audrey R. , Ridout Kathryn K. , Parade Stephanie H.
Published: October 2016

Early childhood experiences have lasting effects on development, including the risk for psychiatric disorders. Research examining the biologic underpinnings of these associations has revealed the impact of childhood maltreatment on the physiologic stress response and activity of the hypothalamus–pituitary–adrenal axis. A growing body of literature supports the hypothesis that environmental exposures mediate their biological effects via epigenetic mechanisms. Methylation, which is thought to be the most stable form of epigenetic change, is a likely mechanism by which early life exposures have lasting effects. We present recent evidence related to epigenetic regulation of genes involved in hypothalamus–pituitary–adrenal axis regulation, namely, the glucocorticoid receptor gene (nuclear receptor subfamily 3, group C, member 1 [NR3C1]) and FK506 binding protein 51 gene (FKBP5), after childhood adversity and associations with risk for psychiatric disorders. Implications for the development of interventions and future research are discussed.
What Was Socialist Food and What Comes Next?
Authors: MASTEROVOY ANTON
Published: October 2016

Food in the former Soviet Union remains serious political business. In the summer of 2014, in retaliation against Western sanctions imposed in response to the annexation of Crimea, Vladimir Putin's government decreed an odd brand of ‘self-sanctions’ by forbidding the importation of many foodstuffs from the United States and the European Union. Conservative supporters of President Putin sprang into action, exhorting Russian consumers to embrace the opportunity to develop Russian agriculture while Putin's opponents raised the spectre of late Soviet food shortages. Though starvation does not seem like a genuine threat to modern Russia, the fact that these questions are raised at all requires scholars of food to pay attention to Russia and scholars of Russia to view food as an important aspect of the country's history. Serious studies of food in the Soviet Union and under other socialist regimes are particularly worthy of attention since these socio-economic systems, paradoxically, were best known both for proclaiming an end to hunger and for presiding over chronic shortages if not outright famines.
The urban community in fifteenth-century Scotland: language, law and political practice
Authors: HAWES CLAIRE
Published: October 2016

The political culture of Scotland's late medieval towns has been neglected in recent scholarship. This article seeks to provoke discussion through an analysis of communitarian language and its use by urban elites in the fifteenth century. The Scottish urban community, as elsewhere, could be positioned as a location, a legal construct and a group of people. This provided the burgh council with a variety of political tools which could be employed – consciously or otherwise – in order to legitimize its authority.
Addition of granulosa cell mass to the culture medium of oocytes derived from early antral follicles increases oocyte growth, ATP content, and acetylation of H4K12
Authors: Sugiyama Miyako , Sumiya Mei , Shirasuna Koumei , Kuwayama Takehito , Iwata Hisataka
Published: October 2016

The main aim of the present study was to examine the hypothesis that an increase in the number of granulosa cells surrounding developing bovine oocytes results in both high ATP levels and an increase in the acetylation level of H4K12 in oocytes grown in vitro. Oocyte–granulosa cell complexes (OGCs) were collected from early antral follicles (EAFs, 0.4–0.7 mm in diameter), and individually cultured on 96-well plates with or without additional granulosa cell mass that had been prepared from other OGCs. After 16 days of culture, we examined: (i) the rate of antrum formation of the OGCs; (ii) the diameter, maturation, and fertilization rate of the oocytes; and (iii) the ATP content and acetylation level of H4K12 in the oocytes grown in vitro. Granulosa cell mass added to the culture medium contributed to the development of OGCs with a higher rate of antrum formation and oocyte growth. Furthermore, the addition of granulosa cells increased the ATP content and acetylation level of H4K12 in oocytes grown in vitro compared with those developed without addition of granulosa cells. In addition, there was a positive correlation between the ATP content in oocytes grown in vitro and the number of granulosa cells in the corresponding OGCs. The results suggest that granulosa cells play a role not only in the development of OGCs and the growth of oocytes, but also in the determination of ATP content and the acetylation of H4K12 in the oocytes developed in vitro.
The Posthuman in the Anthropocene: A Look through the Aesthetic Field
Authors: Wamberg Jacob , Thomsen Mads Rosendahl
Published: October 2016

The posthuman summons up a complex of both tangible challenges for humanity and a potential shift to a larger, more comprehensive historical perspective on humankind. In this article we will first examine the posthuman in relation to the macro-historical framework of the Anthropocene. Adopting key notions from complexity theory, we argue that the earlier counter-figures of environmental catastrophe (Anthropocene entropy) and corporeal enhancement (transhuman negentropy) should be juxtaposed and blended. Furthermore, we argue for the relevance of a comprehensive aesthetical perspective in a discussion of posthuman challenges. Whereas popular visual culture and many novels illustrate posthuman dilemmas (e.g. the superhero’s oscillation between superhuman and human) in a respect for humanist naturalist norms, avant-garde art performs a posthuman alienation of the earlier negentropic centres of art, a problematization of the human body and mind, that is structurally equivalent to the environmental modification of negentropic rise taking place in the Anthropocene. In a spatial sprawl from immaterial information to material immersion, the autonomous human body and mind, the double apex of organic negentropy, are thus undermined through a dialectics of entropy and order, from abstraction’s indeterminacy to Surrealism’s fragmentation of the body and its interlacing with inorganic things.
Practices, predictors and consequences of expressed breast-milk feeding in healthy full-term infants
Authors: Bai Dorothy Li , Fong Daniel Yee Tak , Lok Kris Yuet Wan , Wong Janet Yuen Ha , Tarrant Marie
Published: October 2016

Objective

To investigate the prevalence and predictors of expressed breast-milk feeding in healthy full-term infants and its association with total duration of breast-milk feeding.

Design

Prospective cohort study.

Setting

In-patient postnatal units of four public hospitals in Hong Kong.

Subjects

A total of 2450 mother–infant pairs were recruited in 2006–2007 and 2011–2012 and followed up prospectively for 12 months or until breast-milk feeding had stopped.

Results

Across the first 6 months postpartum, the rate of exclusive expressed breast-milk feeding ranged from 5·1 to 8·0 % in 2006–2007 and from 18·0 to 19·8 % in 2011–2012. Factors associated with higher rate of exclusive expressed breast-milk feeding included supplementation with infant formula, lack of previous breast-milk feeding experience, having a planned caesarean section delivery and returning to work postpartum. Exclusive expressed breast-milk feeding was associated with an increased risk of early breast-milk feeding cessation when compared with direct feeding at the breast. The hazard ratio (95 % CI) ranged from 1·25 (1·04, 1·51) to 1·91 (1·34, 2·73) across the first 6 months.

Conclusions

Mothers of healthy term infants should be encouraged and supported to feed directly at the breast. Exclusive expressed breast-milk feeding should be recommended only when medically necessary and not as a substitute for feeding directly at the breast. Further research is required to explore mothers’ reasons for exclusive expressed breast-milk feeding and to identify the health outcomes associated with this practice.
Development and validation of the patient evaluation scale (PES) for primary health care in Nigeria
Authors: Ogaji Daprim S. , Giles Sally , Daker-White Gavin , Bower Peter
Published: October 2016

Background

Questionnaires developed for patient evaluation of the quality of primary care are often focussed on primary care systems in developed countries.

Aim

To report the development and validation of the patient evaluation scale (PES) designed for use in the Nigerian primary health care context.

Methods

An iterative process was used to develop and validate the questionnaire using patients attending 28 primary health centres across eight states in Nigeria. The development involved literature review, patient interviews, expert reviews, cognitive testing with patients and waves of quantitative cross-sectional surveys. The questionnaire’s content validity, internal structures, acceptability, reliability and construct validity are reported.

Findings

The full and shortened version of PES with 27 and 18 items, respectively, were developed through these process. The low item non-response from the serial cross-sectional surveys depicts questionnaire’s acceptability among the local population. PES-short form (SF) has Cronbach’s α of 0.87 and three domains (codenamed ‘facility’, ‘organisation’ and ‘health care’) with Cronbach’s αs of 0.78, 0.79 and 0.81, respectively. Items in the multi-dimensional questionnaire demonstrated adequate convergent and discriminant properties. PES-SF scores show significant positive correlation with scores of the full PES and also discriminated population groups in support of a priori hypotheses.

Conclusion

The PES and PES-SF contain items that are relevant to the needs of patients in Nigeria. The good measurement properties of the questionnaire demonstrates its potential usefulness for patient-focussed quality improvement activities in Nigeria. There is still need to translate these questionnaires into major languages in Nigeria and assess their validity against external quality criteria.
How to support teenagers who are losing a parent to cancer: Bereaved young adults' advice to healthcare professionals—A nationwide survey
Authors: Alvariza Anette , Lövgren Malin , Bylund-Grenklo Tove , Hakola Pia , Fürst Carl Johan , Kreicbergs Ulrika
Published: October 2016

Objective:

The loss of a parent to cancer is considered one of the most traumatic events a teenager can experience. Studies have shown that teenagers, from the time of diagnosis, are already extremely worried about the consequences of a parent's cancer but tend to be left to manage these concerns on their own. The present study aimed to explore young adults' advice to healthcare professionals on how to support teenagers who are losing a parent to cancer.

Methods:

This work derives from a Swedish nationwide survey and employs a qualitative approach with a descriptive/interpretive design to obtain answers to an open-ended question concerning advice to healthcare professionals. Of the 851 eligible young adults who had lost a parent to cancer when they were 13–16 years of age within the previous 6 to 9 years, 622 participated in our survey (response rate = 73%). Of these 622 young adults, 481 responded to the open-ended question about what advice to give healthcare professionals.

Results:

Four themes emerged: (1) to be seen and acknowledged; (2) to understand and prepare for illness, treatment, and the impending death; (3) to spend time with the ill parent, and (4) to receive support tailored to the individual teenager's needs.

Significance of Results:

This nationwide study contributes hands-on suggestions to healthcare staff regarding attitudes, communication, and support from the perspective of young adults who, in their teenage years, lost a parent to cancer. Teenagers may feel better supported during a parent's illness if healthcare professionals take this manageable advice forward into practice and see each teenager as individuals; explain the disease, its treatments, and consequences; encourage teenagers to spend time with their ill parent; and recommend sources of support.
Immune-modulating effects of bevacizumab in metastatic non-small-cell lung cancer patients
Authors: Martino EC , Misso G , Pastina P , Costantini S , Vanni F , Gandolfo C , Botta C , Capone F , Lombardi A , Pirtoli L , Tassone P , Ulivieri C , Tagliaferri P , Cusi MG , Caraglia M , Correale P
Published: October 2016

The mPEBev is an anticancer regimen which combines a chemotherapy doublet, based on cisplatin and oral etoposide (mPE), with bevacizumab (mPEBev), a mAb targeting the vasculo-endothelial growth factor (VEGF). In previous studies, this regimen showed powerful anti-angiogenetic effects and significant antitumor activity in metastatic non-small-cell lung cancer (mNSCLC) patients. We also recorded the best benefit in patients exhibiting low-systemic inflammatory profile at baseline. On these bases, we hypothesized that mPEBev antitumor activity could be partially related to bevacizumab-associated immunological effects. For this reason, we performed an immunological monitoring in 59 out of 120 stage IIIb-IV NSCLC patients enrolled in the BEVA2007 phase II trial, who received fractioned cisplatin (30 mg/sqm days 1-3q21) and oral etoposide (50 mg, days 1-15q21) (mPE doublet) ±bevacizumab. In this group of patients, 12 received the mPE doublet alone and 47 the doublet in combination with bevacizumab (5 mg/kg on the day 3q21; mPEBev regimen). Blood cell counts, serum analysis, multiplex cytokine assay and immunocytofluorimetric analysis, performed on baseline and post-treatment on blood samples from these patients, revealed that bevacizumab addition to the doublet decreased levels of pro-angiogenic (VEGF, Angiostatin-1 and Follistatin) and inflammatory cytokines (interferon (IFN)γ, IL4 and IL17), improved in vivo and in vitro cytotoxic T-lymphocytes (CTL) response and promoted dendritic cell activation. These results suggest that the mPEBev regimen improve the micro-environmental conditions for an efficient antigen-specific CTL response, making it a feasible candidate regimen to be assessed in combination with immune-checkpoint inhibitors in NSCLC patients.
FoxO1 interacts with transcription factor EB and differentially regulates mitochondrial uncoupling proteins via autophagy in adipocytes
Authors: Liu Longhua , Tao Zhipeng , Zheng Louise D , Brooke Joseph P , Smith Cayleen M , Liu Dongmin , Long Yun Chau , Cheng Zhiyong
Published: October 2016

Mitochondrial uncoupling proteins (UCPs) are inducible and play an important role in metabolic and redox homeostasis. Recent studies have suggested that FoxO1 controls mitochondrial biogenesis and morphology, but it remains largely unknown how FoxO1 may regulate mitochondrial UCPs. Here we show that FoxO1 interacted with transcription factor EB (Tfeb), a key regulator of autophagosome and lysosome, and mediated the expression of UCP1, UCP2 and UCP3 differentially via autophagy in adipocytes. UCP1 was down-regulated but UCP2 and UCP3 were upregulated during adipocyte differentiation, which was associated with increased Tfeb and autophagy activity. However, inhibition of FoxO1 suppressed Tfeb and autophagy, attenuating UCP2 and UCP3 but increasing UCP1 expression. Pharmacological blockade of autophagy recapitulated the effects of FoxO1 inhibition on UCPs. Chromatin immunoprecipitation assay demonstrated that FoxO1 interacted with Tfeb by directly binding to its promoter, and silencing FoxO1 led to drastic decrease in Tfeb transcript and protein levels. These data provide the first line of evidence that FoxO1 interacts with Tfeb to regulate autophagy and UCP expression in adipocytes. Dysregulation of FoxO1→autophagy→UCP pathway may account for metabolic changes in obesity.
Protein arginylation regulates cellular stress response by stabilizing HSP70 and HSP40 transcripts
Authors: Deka Kamalakshi , Singh Archana , Chakraborty Surajit , Mukhopadhyay Rupak , Saha Sougata
Published: October 2016

ATE1-mediated post-translational addition of arginine to a protein has been shown to regulate activity, interaction, and stability of the protein substrates. Arginylation has been linked to many different stress conditions, namely ER stress, cytosolic misfolded protein stress, and nitrosative stress. However, clear understanding about the effect of arginylation in cellular stress responses is yet to emerge. In this study, we investigated the role of arginylation in heat-stress response. Our findings suggest that Ate1 knock out (KO) cells are more susceptible to heat stress compared with its wild-type counterparts due to the induction of apoptosis in KO cells. Gene expression analysis of inducible heat-shock proteins (HSP70.1, HSP70.3, and HSP40) showed induction of these genes in KO cells early in the heat shock, but were drastically diminished at the later period of heat shock. Further analysis revealed that loss of ATE1 drastically reduced the stability of all three HSP mRNAs. These phenotypes were greatly restored by overexpression of Ate1 in KO cells. Our findings show that arginylation plays a protective role during heat stress by regulating HSP gene expression and mRNA stability.
Glatiramer acetate treatment normalized the monocyte activation profile in MS patients to that of healthy controls
Authors: Chuluundorj Delgertsetseg , Harding Scott A , Abernethy David , La Flamme Anne Camille
Published: October 2016

Multiple sclerosis (MS) is an immune‐mediated disease of the central nervous system, and monocytes contribute to MS‐associated neuroinflammation. While classically activated monocytes promote inflammation, type II‐activated monocytes improve the course of MS. This study investigated type II activation of monocytes and their two main subsets, namely CD14+ (CD14++CD16− subset) and CD16+ monocytes (CD14+CD16+ subset), by glatiramer acetate (GA) or intravenous immunoglobulin‐associated immune complexes (IC), both of which are known MS treatments. Total monocytes and subsets were isolated from peripheral blood mononuclear cells (PBMC) of healthy controls, untreated MS patients (MS) and GA‐treated MS patients (GA‐MS). In contrast to the more activated ex vivo profile of monocytes from the MS group, monocytes from the GA‐MS group resembled those from healthy controls. In vitro type II activation with GA primarily reduced CD40, CD86 and IL‐12p40 whereas type II activation with IC consistently reduced CD40 but increased interleukin‐10 (IL‐10), suggesting that the GA and IC activation pathways are distinct. Moreover, while GA treatment reduced IL‐12p40 by both CD14+ and CD16+ subsets, IC treatment only enhanced IL‐10 by the CD16+ subset. Further analysis of the CD16+ subset revealed that MS patients had a greatly expanded CD14+CD16int population while both CD14+CD16int and CD14lowCD16high monocyte populations were expanded in GA‐MS patients. Finally, a global analysis of the ex vivo monocyte data indicated that GA treatment distinctly altered the monocyte profile of MS patients, further supporting the idea that GA directly targets monocytes.
A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors
Authors: Averaimo Stefania , Assali Ahlem , Ros Oriol , Couvet Sandrine , Zagar Yvrick , Genescu Ioana , Rebsam Alexandra , Nicol Xavier
Published: October 2016

The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains of the plasma membrane termed lipid rafts. Whether such domains enable local intracellular signalling at the submicrometre scale in developing neurons and are required for shaping the nervous system connectivity in vivo remains controversial. Here, we report a role for lipid rafts in generating domains of local cAMP signalling in axonal growth cones downstream of ephrin-A repulsive guidance cues. Ephrin-A-dependent retraction of retinal ganglion cell axons involves cAMP signalling restricted to the vicinity of lipid rafts and is independent of cAMP modulation outside of this microdomain. cAMP modulation near lipid rafts controls the pruning of ectopic axonal branches of retinal ganglion cells in vivo, a process requiring intact ephrin-A signalling. Together, our findings indicate that lipid rafts structure the subcellular organization of intracellular cAMP signalling shaping axonal arbors during the nervous system development.
Localized force application reveals mechanically sensitive domains of Piezo1
Authors: Wu Jason , Goyal Raman , Grandl Jörg
Published: October 2016

Piezos are mechanically activated ion channels that function as sensors of touch and pressure in various cell types. However, the precise mechanism and structures mediating mechanical activation and subsequent inactivation have not yet been identified. Here we use magnetic nanoparticles as localized transducers of mechanical force in combination with pressure-clamp electrophysiology to identify mechanically sensitive domains important for activation and inactivation.
Noise reduction facilitated by dosage compensation in gene networks
Authors: Peng Weilin , Song Ruijie , Acar Murat
Published: October 2016

Genetic noise together with genome duplication and volume changes during cell cycle are significant contributors to cell-to-cell heterogeneity. How can cells buffer the effects of these unavoidable epigenetic and genetic variations on phenotypes that are sensitive to such variations? Here we show that a simple network motif that is essential for network-dosage compensation can reduce the effects of extrinsic noise on the network output. Using natural and synthetic gene networks with and without the network motif, we measure gene network activity in single yeast cells and find that the activity of the compensated network is significantly lower in noise compared with the non-compensated network. A mathematical analysis provides intuitive insights into these results and a novel stochastic model tracking cell-volume and cell-cycle predicts the experimental results. Our work implies that noise is a selectable trait tunable by evolution.
The evolution of antimicrobial peptide resistance in Pseudomonas aeruginosa is shaped by strong epistatic interactions
Authors: Jochumsen Nicholas , Marvig Rasmus L. , Damkiær Søren , Jensen Rune Lyngklip , Paulander Wilhelm , Molin Søren , Jelsbak Lars , Folkesson Anders
Published: October 2016

Colistin is an antimicrobial peptide that has become the only remaining alternative for the treatment of multidrug-resistant Gram-negative bacterial infections, but little is known of how clinical levels of colistin resistance evolve. We use in vitro experimental evolution and whole-genome sequencing of colistin-resistant Pseudomonas aeruginosa isolates from cystic fibrosis patients to reconstruct the molecular evolutionary pathways open for high-level colistin resistance. We show that the evolution of resistance is a complex, multistep process that requires mutation in at least five independent loci that synergistically create the phenotype. Strong intergenic epistasis limits the number of possible evolutionary pathways to resistance. Mutations in transcriptional regulators are essential for resistance evolution and function as nodes that potentiate further evolution towards higher resistance by functionalizing and increasing the effect of the other mutations. These results add to our understanding of clinical antimicrobial peptide resistance and the prediction of resistance evolution.
Organization of olfactory centres in the malaria mosquito Anopheles gambiae
Authors: Riabinina Olena , Task Darya , Marr Elizabeth , Lin Chun-Chieh , Alford Robert , O'Brochta David A. , Potter Christopher J.
Published: October 2016

Mosquitoes are vectors for multiple infectious human diseases and use a variety of sensory cues (olfactory, temperature, humidity and visual) to locate a human host. A comprehensive understanding of the circuitry underlying sensory signalling in the mosquito brain is lacking. Here we used the Q-system of binary gene expression to develop transgenic lines of Anopheles gambiae in which olfactory receptor neurons expressing the odorant receptor co-receptor (Orco) gene are labelled with GFP. These neurons project from the antennae and maxillary palps to the antennal lobe (AL) and from the labella on the proboscis to the suboesophageal zone (SEZ), suggesting integration of olfactory and gustatory signals occurs in this brain region. We present detailed anatomical maps of olfactory innervations in the AL and the SEZ, identifying glomeruli that may respond to human body odours or carbon dioxide. Our results pave the way for anatomical and functional neurogenetic studies of sensory processing in mosquitoes.
Large Fizeau’s light-dragging effect in a moving electromagnetically induced transparent medium
Authors: Kuan Pei-Chen , Huang Chang , Chan Wei Sheng , Kosen Sandoko , Lan Shau-Yu
Published: October 2016

As one of the most influential experiments on the development of modern macroscopic theory from Newtonian mechanics to Einstein’s special theory of relativity, the phenomenon of light dragging in a moving medium has been discussed and observed extensively in different types of systems. To have a significant dragging effect, the long duration of light travelling in the medium is preferred. Here we demonstrate a light-dragging experiment in an electromagnetically induced transparent cold atomic ensemble and enhance the dragging effect by at least three orders of magnitude compared with the previous experiments. With a large enhancement of the dragging effect, we realize an atom-based velocimeter that has a sensitivity two orders of magnitude higher than the velocity width of the atomic medium used. Such a demonstration could pave the way for motional sensing using the collective state of atoms in a room temperature vapour cell or solid state material.
Methylotrophic methanogenesis discovered in the archaeal phylum Verstraetearchaeota
Authors: Vanwonterghem Inka , Evans Paul N. , Parks Donovan H. , Jensen Paul D. , Woodcroft Ben J. , Hugenholtz Philip , Tyson Gene W.
Published: October 2016

Methanogenesis is the primary biogenic source of methane in the atmosphere and a key contributor to climate change. The long-standing dogma that methanogenesis originated within the Euryarchaeota was recently challenged by the discovery of putative methane-metabolizing genes in members of the Bathyarchaeota, suggesting that methanogenesis may be more phylogenetically widespread than currently appreciated. Here, we present the discovery of divergent methyl-coenzyme M reductase genes in population genomes recovered from anoxic environments with high methane flux that belong to a new archaeal phylum, the Verstraetearchaeota. These archaea encode the genes required for methylotrophic methanogenesis, and may conserve energy using a mechanism similar to that proposed for the obligate H2-dependent methylotrophic Methanomassiliicoccales and recently described Candidatus ‘Methanofastidiosa’. Our findings indicate that we are only beginning to understand methanogen diversity and support an ancient origin for methane metabolism in the Archaea, which is changing our understanding of the global carbon cycle.
Neural Activities Underlying the Feedback Express Salience Prediction Errors for Appetitive and Aversive Stimuli
Authors: Gu Yan , Hu Xueping , Pan Weigang , Yang Chun , Wang Lijun , Li Yiyuan , Chen Antao
Published: October 2016

Feedback information is essential for us to adapt appropriately to the environment. The feedback-related negativity (FRN), a frontocentral negative deflection after the delivery of feedback, has been found to be larger for outcomes that are worse than expected and it reflects a reward prediction error derived from the midbrain dopaminergic projections to the anterior cingulate cortex (ACC), as stated in reinforcement learning theory. In contrast, the prediction of response-outcome (PRO) model claims that the neural activity in the mediofrontal cortex (mPFC), especially the ACC, is sensitive to the violation of expectancy, irrespective of the valence of feedback. Additionally, increasing evidence has demonstrated significant activities in the striatum, anterior insula and occipital lobe for unexpected outcomes independently of their valence. Thus, the neural mechanism of the feedback remains under dispute. Here, we investigated the feedback with monetary reward and electrical pain shock in one task via functional magnetic resonance imaging. The results revealed significant prediction-error-related activities in the bilateral fusiform gyrus, right middle frontal gyrus and left cingulate gyrus for both money and pain. This implies that some regions underlying the feedback may signal a salience prediction error rather than a reward prediction error.
Time-resolved metabolomics analysis of individual differences during the early stage of lipopolysaccharide-treated rats
Authors: Dai Die , Gao Yiqiao , Chen Jiaqing , Huang Yin , Zhang Zunjian , Xu Fengguo
Published: October 2016

Lipopolysaccharide (LPS) can lead to uncontrollable cytokine production and eventually cause fatal sepsis syndrome. Individual toxicity difference of LPS has been widely reported. In our study we observed that two thirds of the rats (24/36) died at a given dose of LPS, while the rest (12/36) survived. Tracking the dynamic metabolic change in survival and non-survival rats in the early stage may reveal new system information to understand the inter-individual variation in response to LPS. As the time-resolved datasets are very complex and no single method can elucidate the problem clearly and comprehensively, the static and dynamic metabolomics methods were employed in combination as cross-validation. Intriguingly, some common results have been observed. Lipids were the main different metabolites between survival and non-survival rats in pre-dose serum and in the early stage of infection with LPS. The LPS treatment led to S-adenosly-methionine and total cysteine individual difference in early stage, and subsequent significant perturbations in energy metabolism and oxidative stress. Furthermore, cytokine profiles were analyzed to identify potential biological associations between cytokines and specific metabolites. Our collective findings may provide some heuristic guidance for elucidating the underlying mechanism of individual difference in LPS-mediated disease.