bluuebunny/crossref_metadata_2025 huggingface dataset:
Re St Thomas the Apostle, Killinghall
Authors: Willink David
Published: May 2024

The petitioners sought a faculty for the disposal by sale of two damp-affected paintings. The DAC recommended the proposal. The court, out of an abundance of caution, directed consultation with the CBC pursuant to rule 9.6 of the Faculty Jurisdiction Rules 2015 (under which consultation with the CBC is mandatory on a petition for the conservation, alteration or disposal of an article of special historic, architectural, archaeological or artistic interest). The CBC preferred to see the conservation and retention of the paintings, there being a strong presumption against the sale of church treasures. The petitioners described the CBC's position as unrealistic, and the DAC noted that the paintings were of limited significance; they would continue to deteriorate if kept in the church; and the petitioners had neither the funds nor the inclination to seek them to fund their restoration.
Re St Michael and All Angels, Bampton
Authors: Stuart Jack
Published: May 2024

This Grade 1-listed mediaeval church had a substantial Georgian reredos. It dominated the east end of the church, hiding three-quarters of the plain-glazed east window. In 2012 a faculty permitted its temporary removal to another location in the church while essential works were done to the chancel. Following this, the consequent increase in light coming from the windows was welcomed by petitioners, and fragments of medieval wall painting were discovered behind plasterwork which the reredos had previously protected. The petitioners now wished to make the temporary removal of the reredos permanent, relocating it to hang above the west door.
Re St Michael le Belfrey, York
Authors: Murray Philip
Published: May 2024

St Michael le Belfrey (‘the Belfrey’) is a 16th century parish church in the shadow of York Minster. It sits in the charismatic evangelical tradition of the Church of England. With a large, young and vibrant congregation, the Belfrey is a Resource Church and plays a significant role in the life of the Diocese of York, the Northern Province and, more broadly, the Church of England. Through a petition described as ‘of the highest quality’, it sought a faculty for a dramatic re-ordering of its interior, proposals that had been at least 14 years in the development.
When a doctrine is not a doctrine: understating the intersection of civil and canon law and the ‘doctrine’ of marriage in the Anglican Church of Australia
Authors: Barker Renae
Published: May 2024

This article explores the interpretation and application of the term ‘doctrine’ within the Anglican Church of Australia and its implications in Australian civil law, particularly anti-discrimination legislation. It examines the tension between (1) the constitutional definition in the Church's constitution and (2) broader interpretations found in General Synod resolutions. The anxiety evident in the General Synod resolutions underscores ongoing debates within the Church about same-sex marriage and relationships and the application of secular exemptions in anti-discrimination legislation. The article concludes that the civil law definition of the term ‘doctrine’ is wide enough to encompass both the Anglican Church of Australia's constitutional definition and the broader meaning found in General Synod resolutions. Nevertheless, care needs to be taken by the Church to avoid the risk of civil courts being called upon to engage in their own exegesis of scripture, and thereby come to conclusions which are at odds with the avowed beliefs of the Church.
Unified framework for the separation property in binary phase-segregation processes with singular entropy densities
Authors: Gal Ciprian G. , Poiatti Andrea
Published: May 2024

This paper investigates the separation property in binary phase-segregation processes modelled by Cahn-Hilliard type equations with constant mobility, singular entropy densities and different particle interactions. Under general assumptions on the entropy potential, we prove the strict separation property in both two and three-space dimensions. Namely, in 2D, we notably extend the minimal assumptions on the potential adopted so far in the literature, by only requiring a mild growth condition of its first derivative near the singular points
$\pm 1$
, without any pointwise additional assumption on its second derivative. For all cases, we provide a compact proof using De Giorgi’s iterations. In 3D, we also extend the validity of the asymptotic strict separation property to the case of fractional Cahn-Hilliard equation, as well as show the validity of the separation when the initial datum is close to an ‘energy minimizer’. Our framework offers insights into statistical factors like particle interactions, entropy choices and correlations governing separation, with broad applicability.
Tourism Diplomacy in Cold War Europe: Symbolic Gestures, Cultural Exchange and Human Rights
Authors: Bechmann Pedersen Sune , Stanoeva Elitza
Published: May 2024

The post-war boom in international travel made tourism a question for international diplomacy. Focusing on the growth of bilateral tourism agreements during the Cold War, this article shows how the meaning of tourism was negotiated by and between governments on either side of the East–West divide. While previous research on tourism in the Cold War has focused on the threat tourist traffic posed to national security in socialist states, the present study also considers the dilemmas it presented to liberal democracies. The article analyses the intersections of tourism with issues of foreign trade, cultural exchange and human contacts, which shaped the contestations over tourism throughout the Cold War.
Double-orifice tricuspid valve: case series and literature review
Authors: Haughian Brendan T. , Khoo Nee S. , Lin Lily Q.
Published: May 2024

The double-orifice tricuspid valve (DOTV) is a rare lesion. We present a series of three patients encountered at the Stollery Children’s Hospital with a diagnosis of DOTV on 2D and/or 3D echocardiography. The patient’s medical records are reviewed for presentation history, investigative findings, and clinical course. We discuss the cases in the context of a complete review of all literature documenting cases of DOTV. In the majority of cases, the lesion is relatively benign, with little change in valve function over the short to medium term, and outcomes are determined largely by significant concomitant heart defects.
Panic-agoraphobic symptoms in adults with ASD: the role of ruminative thinking and inflexibility
Authors: Dell’Osso Liliana , Amatori Giulia , Bonelli Chiara , Nardi Benedetta , Massimetti Enrico , Cremone Ivan Mirko , Carpita Barbara
Published: May 2024

Background

Scientific literature has highlighted the link between autism spectrum disorder (ASD) and anxiety disorders, but few studies have delved into the relationship between ASD and panic-agoraphobic disorders. The aim of this study is to investigate the relationship between autism spectrum and panic-agoraphobic symptoms, examining whether and which autistic domains are predictive of the presence of specific panic-agoraphobic symptoms.

Materials and methods

Forty-five adult subjects with ASD and 50 healthy controls (HCs) were evaluated through the Structured Clinical Interview for DSM-5, Research Version and assessed with the Adult Autism Subthreshold Spectrum (AdAS Spectrum) and the Panic-Agoraphobic – Short Version (PAS-SV) questionnaires. Statistical analyses included Mann–Whitney U test, chi-square test, and a set of linear and logistic regression analyses.

Results

The PAS-SV total and domain scores were significantly higher in the ASD group than in the HC group. A higher AdAS total score appeared to be predictive of a higher PAS-SV total score. The AdAS domain Restricted Interests and Rumination would increase the risk of obtaining higher PAS-SV total and domain scores. Conversely, the AdAS Spectrum domain Inflexibility and Adherence to Routine would predict lower total PAS-SV score.

Conclusion

This study revealed a greater representation of panic-agoraphobic symptoms in adults with ASD, as well as an increased risk of showing such symptoms in the presence of significant autistic traits. Restricted interests and ruminative thinking emerged as predominant risk factors for panic-agoraphobic manifestations.
General practitioner preferences for telehealth consultations in Australia: a pilot survey and discrete choice experiment
Authors: De Guzman Keshia R. , Smith Anthony C. , Snoswell Centaine L.
Published: May 2024

Aim:

To identify and quantify general practitioner (GP) preferences related to service attributes of clinical consultations, including telehealth consultations, in Australia.

Background:

GPs have been increasingly using telehealth to deliver patient care since the onset of the 2019 coronavirus disease (COVID-19) pandemic. GP preferences for telehealth service models will play an important role in the uptake and sustainability of telehealth services post-pandemic.

Methods:

An online survey was used to ask GPs general telehealth questions and have them complete a discrete choice experiment (DCE). The DCE elicited GP preferences for various service attributes of telehealth (telephone and videoconference) consultations. The DCE investigated five service attributes, including consultation mode, consultation purpose, consultation length, quality of care and rapport, and patient co-payment. Participants were presented with eight choice sets, each containing three options to choose from. Descriptive statistics was used, and mixed logit models were used to estimate and analyse the DCE data.

Findings:

A total of 60 GPs fully completed the survey. Previous telehealth experiences impacted direct preferences towards telehealth consultations across clinical presentations, although in-person modes were generally favoured (in approximately 70% of all scenarios). The DCE results lacked statistical significance which demonstrated undiscernible differences between GP preferences for some service attributes. However, it was found that GPs prefer to provide a consultation with good quality care and rapport (P < 002). GPs would also prefer to provide care to their patients rather than decline a consultation due to consultation mode, length or purpose (P < 0.0001). Based on the findings, GPs value the ability to provide high-quality care and develop rapport during a clinical consultation. This highlights the importance of recognising value-based care for future policy reforms, to ensure continued adoption and sustainability of GP telehealth services in Australia.
Transformative process theory
Authors: Fowkes James
Published: May 2024

Transformative constitutionalism and process theory are generally seen as worlds apart. But they may be more compatible than we think. A transformative understanding of process is very broad, but it represents a natural extension of the line already being taken by contemporary process accounts intent on expanding the theory to fit global practice. It can help us to understand why an expansion based on and including a wider set of justiciable process concerns has proved difficult to limit. Conversely, transformative constitutionalism badly needs a better account of judicial restraint to balance its preoccupation with judicial boldness. Since it shares with process theory a deep concern with democracy, it can naturally draw on process accounts to understand its own limits. Democracy-seeking review, in aiming to build as well as protect democratic capacity, needs to be as concerned with restraint as intervention, depending on the context. Working out a transformative process theory is therefore at least an exercise instructive to either side, and it can offer a way to overcome divides that hamper global engagement with these core constitutional issues.
The Moon’s Mantle Did a Flip—and Scientists May Now Have Evidence
Authors: Barbuzano Javier
Published: May 2024

For decades, a lunar whodunit has puzzled scientists: Did the Moon’s internal layers flip during its formation? Old data might hold the evidence to solve this cold case.
Reflecting on 5 Years at the Helm of JGR: Planets
Authors: Mont�si Laurent
Published: May 2024

The outgoing Editor-in-Chief of JGR: Planets reflects on the position of planetary sciences within AGU and the dedication of the planetary science community to supporting sound and insightful science.
Entangled photon pair generation in an integrated SiC platform
Authors: Rahmouni Anouar , Wang Ruixuan , Li Jingwei , Tang Xiao , Gerrits Thomas , Slattery Oliver , Li Qing , Ma Lijun
Published: May 2024

Entanglement plays a vital role in quantum information processing. Owing to its unique material properties, silicon carbide recently emerged as a promising candidate for the scalable implementation of advanced quantum information processing capabilities. To date, however, only entanglement of nuclear spins has been reported in silicon carbide, while an entangled photon source, whether it is based on bulk or chip-scale technologies, has remained elusive. Here, we report the demonstration of an entangled photon source in an integrated silicon carbide platform for the first time. Specifically, strongly correlated photon pairs are efficiently generated at the telecom C-band wavelength through implementing spontaneous four-wave mixing in a compact microring resonator in the 4H-silicon-carbide-on-insulator platform. The maximum coincidence-to-accidental ratio exceeds 600 at a pump power of 0.17 mW, corresponding to a pair generation rate of (9 ± 1) × 103 pairs/s. Energy-time entanglement is created and verified for such signal-idler photon pairs, with the two-photon interference fringes exhibiting a visibility larger than 99%. The heralded single-photon properties are also measured, with the heralded g(2)(0) on the order of 10−3, demonstrating the SiC platform as a prospective fully integrated, complementary metal-oxide-semiconductor compatible single-photon source for quantum applications.
Resolvin E1 improves efferocytosis and rescues severe aplastic anemia in mice
Authors: Grazda Rachel , Seyfried Allison N. , Maddipati Krishna Rao , Fredman Gabrielle , MacNamara Katherine C.
Published: May 2024

Severe aplastic anemia (SAA) is a rare, fatal disease characterized by severe cytopenias and loss of hematopoietic stem cells (HSCs). Immune-mediated destruction and inflammation are known drivers of SAA, however, the underlying mechanisms driving persistent inflammation are unknown. Current treatments for SAA rely on immunosuppressive therapies or HSC transplantation, however, these treatments are not always effective. Using an established mouse model of SAA, we observed a significant increase in apoptotic cells within the bone marrow (BM) and impaired efferocytosis in SAA mice, relative to radiation controls. Single-cell transcriptomic analysis revealed heterogeneity among BM monocytes and unique populations emerged during SAA characterized by increased inflammatory signatures and significantly increased expression of Sirpa and Cd47. CD47, a “don’t eat me” signal, was increased on both live and apoptotic BM cells, concurrent with markedly increased expression of signal regulatory protein alpha (SIRPα) on monocytes. Functionally, SIRPα blockade improved cell clearance and reduced accumulation of CD47-positive apoptotic cells. Lipidomic analysis revealed a reduction in the precursors of specialized pro-resolving lipid mediators (SPMs) and increased prostaglandins in the BM during SAA, indicative of impaired inflammation resolution. Specifically, 18-HEPE, a precursor of E-series resolvins, was significantly reduced in SAA-induced mice relative to radiation controls. Treatment of SAA mice with Resolvin E1 (RvE1) improved efferocytic function, BM cellularity, platelet output, and survival. Our data suggest that impaired efferocytosis and inflammation resolution contributes to SAA progression and demonstrate that SPMs, such as RvE1, offer new and/or complementary treatments for SAA that do not rely on immune suppression.
Effect of crystal facets in plasmonic catalysis
Authors: Kang Yicui , João Simão M. , Lin Rui , Liu Kang , Zhu Li , Fu Junwei , Cheong Weng-Chon , Lee Seunghoon , Frank Kilian , Nickel Bert , Liu Min , Lischner Johannes , Cortés Emiliano
Published: May 2024

While the role of crystal facets is well known in traditional heterogeneous catalysis, this effect has not yet been thoroughly studied in plasmon-assisted catalysis, where attention has primarily focused on plasmon-derived mechanisms. Here, we investigate plasmon-assisted electrocatalytic CO2 reduction using different shapes of plasmonic Au nanoparticles - nanocube (NC), rhombic dodecahedron (RD), and octahedron (OC) - exposing {100}, {110}, and {111} facets, respectively. Upon plasmon excitation, Au OCs doubled CO Faradaic efficiency (FECO) and tripled CO partial current density (jCO) compared to a dark condition, with NCs also improving under illumination. In contrast, Au RDs maintained consistent performance irrespective of light exposure, suggesting minimal influence of light on the reaction. Temperature experiments ruled out heat as the main factor to explain such differences. Atomistic simulations and electromagnetic modeling revealed higher hot carrier abundance and electric field enhancement on Au OCs and NCs than RDs. These effects now dominate the reaction landscape over the crystal facets, thus shifting the reaction sites when comparing dark and plasmon-activated processes. Plasmon-assisted H2 evolution reaction experiments also support these findings. The dominance of low-coordinated sites over facets in plasmonic catalysis suggests key insights for designing efficient photocatalysts for energy conversion and carbon neutralization.
Sputum culture reversion in longer treatments with bedaquiline, delamanid, and repurposed drugs for drug-resistant tuberculosis
Authors: Kho Sooyeon , Seung Kwonjune J. , Huerga Helena , Bastard Mathieu , Khan Palwasha Y. , Mitnick Carole D. , Rich Michael L. , Islam Shirajul , Zhizhilashvili Dali , Yeghiazaryan Lusine , Nikolenko Elena Nikolaevna , Zarli Khin , Adnan Sana , Salahuddin Naseem , Ahmed Saman , Vargas Zully Haydee Ruíz , Bekele Amsalu , Shaimerdenova Aiman , Tamirat Meseret , Gelin Alain , Vilbrun Stalz Charles , Hewison Catherine , Khan Uzma , Franke Molly
Published: May 2024

Sputum culture reversion after conversion is an indicator of tuberculosis (TB) treatment failure. We analyze data from the endTB multi-country prospective observational cohort (NCT03259269) to estimate the frequency (primary endpoint) among individuals receiving a longer (18-to-20 month) regimen for multidrug- or rifampicin-resistant (MDR/RR) TB who experienced culture conversion. We also conduct Cox proportional hazard regression analyses to identify factors associated with reversion, including comorbidities, previous treatment, cavitary disease at conversion, low body mass index (BMI) at conversion, time to conversion, and number of likely-effective drugs. Of 1,286 patients, 54 (4.2%) experienced reversion, a median of 173 days (97-306) after conversion. Cavitary disease, BMI < 18.5, hepatitis C, prior treatment with second-line drugs, and longer time to initial culture conversion were positively associated with reversion. Reversion was uncommon. Those with cavitary disease, low BMI, hepatitis C, prior treatment with second-line drugs, and in whom culture conversion is delayed may benefit from close monitoring following conversion.
ARTseq-FISH reveals position-dependent differences in gene expression of micropatterned mESCs
Authors: Hu Xinyu , van Sluijs Bob , García-Blay Óscar , Stepanov Yury , Rietrae Koen , Huck Wilhelm T. S. , Hansen Maike M. K.
Published: May 2024

Differences in gene-expression profiles between individual cells can give rise to distinct cell fate decisions. Yet how localisation on a micropattern impacts initial changes in mRNA, protein, and phosphoprotein abundance remains unclear. To identify the effect of cellular position on gene expression, we developed a scalable antibody and mRNA targeting sequential fluorescence in situ hybridisation (ARTseq-FISH) method capable of simultaneously profiling mRNAs, proteins, and phosphoproteins in single cells. We studied 67 (phospho-)protein and mRNA targets in individual mouse embryonic stem cells (mESCs) cultured on circular micropatterns. ARTseq-FISH reveals relative changes in both abundance and localisation of mRNAs and (phospho-)proteins during the first 48 hours of exit from pluripotency. We confirm these changes by conventional immunofluorescence and time-lapse microscopy. Chemical labelling, immunofluorescence, and single-cell time-lapse microscopy further show that cells closer to the edge of the micropattern exhibit increased proliferation compared to cells at the centre. Together these data suggest that while gene expression is still highly heterogeneous position-dependent differences in mRNA and protein levels emerge as early as 12 hours after LIF withdrawal.
Gas adsorption and framework flexibility of CALF-20 explored via experiments and simulations
Authors: Oktavian Rama , Goeminne Ruben , Glasby Lawson T. , Song Ping , Huynh Racheal , Qazvini Omid Taheri , Ghaffari-Nik Omid , Masoumifard Nima , Cordiner Joan L. , Hovington Pierre , Van Speybroeck Veronique , Moghadam Peyman Z.
Published: May 2024

In 2021, Svante, in collaboration with BASF, reported successful scale up of CALF-20 production, a stable MOF with high capacity for post-combustion CO2 capture which exhibits remarkable stability towards water. CALF-20’s success story in the MOF commercialisation space provides new thinking about appropriate structural and adsorptive metrics important for CO2 capture. Here, we combine atomistic-level simulations with experiments to study adsorptive properties of CALF-20 and shed light on its flexible crystal structure. We compare measured and predicted CO2 and water adsorption isotherms and explain the role of water-framework interactions and hydrogen bonding networks in CALF-20’s hydrophobic behaviour. Furthermore, regular and enhanced sampling molecular dynamics simulations are performed with both density-functional theory (DFT) and machine learning potentials (MLPs) trained to DFT energies and forces. From these simulations, the effects of adsorption-induced flexibility in CALF-20 are uncovered. We envisage this work would encourage development of other MOF materials useful for CO2 capture applications in humid conditions.
Chiral coordination polymer nanowires boost radiation-induced in situ tumor vaccination
Authors: Huang Zhusheng , Gu Rong , Huang Shiqian , Chen Qian , Yan Jing , Cui Xiaoya , Jiang Haojie , Yao Dan , Shen Chuang , Su Jiayue , Liu Tao , Wu Jinhui , Luo Zhimin , Hu Yiqiao , Yuan Ahu
Published: May 2024

Radiation-induced in situ tumor vaccination alone is very weak and insufficient to elicit robust antitumor immune responses. In this work, we address this issue by developing chiral vidarabine monophosphate-gadolinium nanowires (aAGd-NWs) through coordination-driven self-assembly. We elucidate the mechanism of aAGd-NW assembly and characterize their distinct features, which include a negative surface charge, ultrafine topography, and right-handed chirality. Additionally, aAGd-NWs not only enhance X-ray deposition but also inhibit DNA repair, thereby enhancing radiation-induced in situ vaccination. Consequently, the in situ vaccination induced by aAGd-NWs sensitizes radiation enhances CD8+ T-cell-dependent antitumor immunity and synergistically potentiates the efficacy immune checkpoint blockade therapies against both primary and metastatic tumors. The well-established aAGd-NWs exhibit exceptional therapeutic capacity and biocompatibility, offering a promising avenue for the development of radioimmunotherapy approaches.
Unraveling dispersion and buoyancy dynamics around radial A + B → C reaction fronts: microgravity experiments and numerical simulations
Authors: Stergiou Yorgos , Escala Darío M. , Papp Paszkál , Horváth Dezső , Hauser Marcus J. B. , Brau Fabian , De Wit Anne , Tóth Ágota , Eckert Kerstin , Schwarzenberger Karin
Published: May 2024

Radial Reaction–Diffusion–Advection (RDA) fronts for A + B → C reactions find wide applications in many natural and technological processes. In liquid solutions, their dynamics can be perturbed by buoyancy-driven convection due to concentration gradients across the front. In this context, we conducted microgravity experiments aboard a sounding rocket, in order to disentangle dispersion and buoyancy effects in such fronts. We studied experimentally the dynamics due to the radial injection of A in B at a constant flow rate, in absence of gravity. We compared the obtained results with numerical simulations using either radial one– (1D) or two–dimensional (2D) models. We showed that gravitational acceleration significantly distorts the RDA dynamics on ground, even if the vertical dimension of the reactor and density gradients are small. We further quantified the importance of such buoyant phenomena. Finally, we showed that 1D numerical models with radial symmetry fail to predict the dynamics of RDA fronts in thicker geometries, while 2D radial models are necessary to accurately describe RDA dynamics where Taylor–Aris dispersion is significant.
A quantum critical Bose gas of magnons in the quasi-two-dimensional antiferromagnet YbCl3 under magnetic fields
Authors: Matsumoto Yosuke , Schnierer Simon , Bruin Jan A. N. , Nuss Jürgen , Reiss Pascal , Jackeli George , Kitagawa Kentaro , Takagi Hidenori
Published: May 2024

Bose–Einstein condensation (BEC) is a quantum phenomenon in which a macroscopic number of bosons occupy the lowest energy state and acquire coherence at low temperatures. In three-dimensional antiferromagnets, a magnetic-field-induced transition has been successfully described as a magnon BEC. For a strictly two-dimensional (2D) system, it is known that BEC cannot take place due to the presence of a finite density of states at zero energy. However, in a realistic quasi-2D magnet consisting of stacked magnetic layers, a small but finite interlayer coupling stabilizes marginal BEC but such that 2D physics is still expected to dominate. This 2D-limit BEC behaviour has been reported in a few materials but only at very high magnetic fields that are difficult to access. The honeycomb S = 1/2 Heisenberg antiferromagnet YbCl3 exhibits a transition to a fully polarized state at a relatively low in-plane magnetic field. Here, we demonstrate the formation of a quantum critical 2D Bose gas at the transition field, which, with lowering the field, experiences a BEC marginally stabilized by an extremely small interlayer coupling. Our observations establish YbCl3, previously a Kitaev quantum spin liquid material, as a realization of a quantum critical BEC in the 2D limit.
Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies
Authors: Mastrantonio Valentina , Libro Pietro , Di Martino Jessica , Matera Michele , Bellini Romeo , Castrignanò Tiziana , Urbanelli Sandra , Porretta Daniele
Published: May 2024

We present a de novo transcriptome of the mosquito vector Culex pipiens, assembled by sequences of susceptible and insecticide resistant larvae. The high quality of the assembly was confirmed by TransRate and BUSCO. A mapping percentage until 94.8% was obtained by aligning contigs to Nr, SwissProt, and TrEMBL, with 27,281 sequences that simultaneously mapped on the three databases. A total of 14,966 ORFs were also functionally annotated by using the eggNOG database. Among them, we identified ORF sequences of the main gene families involved in insecticide resistance. Therefore, this resource stands as a valuable reference for further studies of differential gene expression as well as to identify genes of interest for genetic-based control tools.
Chromosomal-level genome assembly and annotation of the tropical sea cucumber Holothuria scabra
Authors: Zhong Shengping , Liu Xujia , Ma Xiaowan , Chen Xiuli , Jiang Yan , Zeng Mengqing , Zhao Longyan , Huang Lianghua , Huang Guoqiang , Zhao Yongzhen , Liu Hongtao , Qiao Ying
Published: May 2024

Holothuria scabra, a commercially valuable yet ecologically vulnerable tropical holothuroid, has experienced a severe decline in its wild populations, especially in China. Genomic resources are crucial for the development of effective genomic breeding projects and stock conservation strategies to restore these natural populations. Until now, a high-quality, chromosome-level reference genome for H. scabra has not been available. Here, we employed Oxford Nanopore and Hi-C sequencing technologies to assemble and annotate a high-quality, chromosome-level reference genome of H. scabra. The final genome comprised 31 scaffolds with a total length of 1.19 Gb and a scaffold N50 length of 53.52 Mb. Remarkably, 1,191.67 Mb (99.95%) of the sequences were anchored to 23 pseudo-chromosomes, with the longest one spanning 79.75 Mb. A total of 34,418 protein-coding genes were annotated in the final genome, with BUSCO analysis revealing 98.01% coverage of metazoa_odb10 genes, marking a significant improvement compared to the previous report. These chromosome-level sequences and annotations will provide an essential genomic basis for further investigation into molecular breeding and conservation management of H. scabra.
PVP/aprepitant microcapsules produced by supercritical antisolvent process
Authors: Zhang Zhuo , Hao Guizhou , Sun Xuemei , Wang Feibo , Zhang Dengbo , Hu Dedong
Published: May 2024

The supercritical antisolvent (SAS) process was a green alternative to improve the low bioavailability of insoluble drugs. However, it is difficult for SAS process to industrialize with limited production capacity. A coaxial annular nozzle was used to prepare the microcapsules of aprepitant (APR) and polyvinylpyrrolidone (PVP) by SAS with N, N-Dimethylformamide (DMF) as solvent. Meanwhile, the effects of polymer/drug ratio, operating pressure, operating temperature and overall concentration on particles morphology, mean particle diameter and size distribution were analyzed. Microcapsules with mean diameters ranging from 2.04 μm and 9.84 μm were successfully produced. The morphology, particle size, thermal behavior, crystallinity, drug content, drug dissolution and residual amount of DMF of samples were analyzed. The results revealed that the APR drug dissolution of the microcapsules by SAS process was faster than the unprocessed APR. Furthermore, the drug powder collected every hour is in the kilogram level, verifying the possibility to scale up the production of pharmaceuticals employing the SAS process from an industrial point of view.
Control of spontaneous charging of sliding water drops by plasma-surface treatment
Authors: Darvish Fahimeh , Shumaly Sajjad , Li Xiaomei , Dong Yun , Diaz Diego , Khani Mohammadreza , Vollmer Doris , Butt Hans-Jürgen
Published: May 2024

Slide electrification is the spontaneous separation of electric charges at the rear of water drops sliding over solid surfaces. This study delves into how surfaces treated with a low-pressure plasma impact water slide electrification. Ar, O2, and N2 plasma treatment reduced the drop charge and contact angles on glass, quartz, and SU-8 coated with 1H,1H,2H,2H-perfluoroctyltrichlorosilane (PFOTS), and polystyrene. Conversely, 64% higher drop charge was achieved using electrode-facing treatment in plasma chamber. Based on the zeta potential, Kelvin potential, and XPS measurements, the plasma effects were attributed to alterations of the topmost layer's chemistry, such as oxidation and etching, and superficially charge deposition. The surface top layer charges were less negative after electrode-facing and more negative after bulk plasma treatment. As a result, the zeta potential was less negative after electrode-facing and more negative after bulk plasma treatment. Although the fluorinated layer was applied after plasma activation, we observed a discernible impact of plasma-glass treatment on drop charging. Plasma surface modification offers a means to adjust drop charges: electrode-facing treatment of the fluorinated layer leads to an enhanced drop charge, while plasma treatment on the substrate prior to fluorination diminishes drop charges, all without affecting contact angles or surface roughness.
Widely-targeted metabolomics and transcriptomics identify metabolites associated with flowering regulation of Choy Sum
Authors: Huang Xinmin , Zhu Yunna , Su Wei , Song Shiwei , Chen Riyuan
Published: May 2024

Choy Sum, a stalk vegetable highly valued in East and Southeast Asia, is characterized by its rich flavor and nutritional profile. Metabolite accumulation is a key factor in Choy Sum stalk development; however, no research has focused on metabolic changes during the development of Choy Sum, especially in shoot tip metabolites, and their effects on growth and flowering. Therefore, in the present study, we used a widely targeted metabolomic approach to analyze metabolites in Choy Sum stalks at the seedling (S1), bolting (S3), and flowering (S5) stages. In total, we identified 493 metabolites in 31 chemical categories across all three developmental stages. We found that the levels of most carbohydrates and amino acids increased during stalk development and peaked at S5. Moreover, the accumulation of amino acids and their metabolites was closely related to G6P, whereas the expression of flowering genes was closely related to the content of T6P, which may promote flowering by upregulating the expressions of BcSOC1, BcAP1, and BcSPL5. The results of this study contribute to our understanding of the relationship between the accumulation of stem tip substances during development and flowering and of the regulatory mechanisms of stalk development in Choy Sum and other related species.
Regulation of cytokine and chemokine expression by histone lysine methyltransferase MLL1 in rheumatoid arthritis synovial fibroblasts
Authors: Okamoto Keita , Araki Yasuto , Aizaki Yoshimi , Tanaka Shinya , Kadono Yuho , Mimura Toshihide
Published: May 2024

Histone lysine methylation is thought to play a role in the pathogenesis of rheumatoid arthritis (RA). We previously reported aberrant expression of the gene encoding mixed-lineage leukemia 1 (MLL1), which catalyzes methylation of histone H3 lysine 4 (H3K4), in RA synovial fibroblasts (SFs). The aim of this study was to elucidate the involvement of MLL1 in the activated phenotype of RASFs. SFs were isolated from synovial tissues obtained from patients with RA or osteoarthritis (OA) during total knee joint replacement. MLL1 mRNA and protein levels were determined after stimulation with tumor necrosis factor α (TNFα). We also examined changes in trimethylation of H3K4 (H3K4me3) levels in the promoters of RA-associated genes (matrix-degrading enzymes, cytokines, and chemokines) and the mRNA levels upon small interfering RNA-mediated depletion of MLL1 in RASFs. We then determined the levels of H3K4me3 and mRNAs following treatment with the WD repeat domain 5 (WDR5)/MLL1 inhibitor MM-102. H3K4me3 levels in the gene promoters were also compared between RASFs and OASFs. After TNFα stimulation, MLL1 mRNA and protein levels were higher in RASFs than OASFs. Silencing of MLL1 significantly reduced H3K4me3 levels in the promoters of several cytokine (interleukin-6 [IL-6], IL-15) and chemokine (C–C motif chemokine ligand 2 [CCL2], CCL5, C-X-C motif chemokine ligand 9 [CXCL9], CXCL10, CXCL11, and C-X3-C motif chemokine ligand 1 [CX3CL1]) genes in RASFs. Correspondingly, the mRNA levels of these genes were significantly decreased. MM-102 significantly reduced the promoter H3K4me3 and mRNA levels of the CCL5, CXCL9, CXCL10, and CXCL11 genes in RASFs. In addition, H3K4me3 levels in the promoters of the IL-6, IL-15, CCL2, CCL5, CXCL9, CXCL10, CXCL11, and CX3CL1 genes were significantly higher in RASFs than OASFs. Our findings suggest that MLL1 regulates the expression of particular cytokines and chemokines in RASFs and is associated with the pathogenesis of RA. These results could lead to new therapies for RA.
Mammalian and avian species quantification in homogenized foods: real time PCR and digital PCR as tools for label compliance controls
Authors: Barbara Bertasi , Michela Tilola , Lucia Mangeri , Roberto Benevenia , Veronica Cappa , Alessandra Scaburri , Sonia Scaramagli , Raffaella Bergami , Pavoni Enrico , Marina Nadia Losio , Simone Peletto
Published: May 2024

Currently food fraud and authenticity of products composition are topics of great concern; ingredients quantification could allow to identify small amounts of contaminats or voluntary addition of improper components. Many molecular methods are available for species identification in foodstuffs but, for a better application, they should not be affected by the interference of other ingredients. The main purpose of this work was to verify the Real Time PCR and the Digital PCR (dPCR) quantification performances on baby food samples, specifically selected for their high miscibility to limit variability; chicken was selected as target to verify the performance of quantification of methods after having spiked the same quantity in different baby foods. The other aims were: (1) to verify a constant genome copies ratio existence between mammalian and avian species (2) to verify the dPCR performance, set up on housekeeping, to quantify mammalian and avian species in commercial products. Digital PCR showed fewer differences respect to Real Time PCR, at the same 15% w/w chicken spiking level. Despite the constant difference between mammalian and avian genome copies, in samples with the same spiking weight, the confidence intervals increasing towards the extreme values, made impossible to use genome copies ratio as a sort of correction factor between species. Finally, the dPCR system using the myostatin housekeeping gene to determine the chicken content seemed reliable to verify the labelling compliance in meat-based commercial products.
Prevalence and contributing factors of anemia in patients with gynecological cancer: a retrospective cohort study
Authors: Ning Kexue , Sun Xingyu , Liu Ling , He Lijuan
Published: May 2024

This retrospective cohort study aimed to determine the prevalence of anemia among patients with gynecological cancer prior to any treatment and to identify contributing factors associated with anemia in this group. We retrospectively analyzed data from female patients aged 18 and above, diagnosed with various forms of gynecological cancer at The Affiliated Hospital of Southwest Medical University between February 2016 and March 2021. Anemia was assessed based on the most recent CBC results before any cancer treatment. Eligibility was based on a definitive histopathological diagnosis. Key variables included demographic details, clinical characteristics, and blood counts, focusing on hemoglobin levels. Statistical analysis was conducted using logistic regression models, and anemia was defined as hemoglobin levels below 12 g/dL for women, according to WHO criteria. Of the 320 participants, a significant prevalence of anemia was found. Correlations between anemia and factors like age, educational level, and biological markers (iron, folic acid, and vitamin B12 levels) were identified. In our study, we found that the prevalence of anemia among patients with gynecological cancer prior to any treatment was 59.06%, indicating a significant health concern within this population. The study highlights a significant prevalence of anemia in patients with gynecological cancer, emphasizing the need for regular hemoglobin screening and individualized management. These findings suggest the importance of considering various characteristics and clinical variables in anemia management among this patient group. Further studies are needed to explore the long-term effects of these factors on patient outcomes and to develop targeted interventions.
Immature characteristics of the East Anatolian Fault Zone from SAR, GNSS and strong motion data of the 2023 Türkiye–Syria earthquake doublet
Authors: Liu Jiao , Huang Chuanchao , Zhang Guohong , Shan Xinjian , Korzhenkov Andrey , Taymaz Tuncay
Published: May 2024

On February 6, 2023, an Mw 7.9 earthquake occurred in the western section of the East Anatolia Fault Zone (EAFZ). It was subsequently followed by an Mw 7.7 earthquake on the northern branch of the EAFZ, known as the Sürgü Fault Zone. Coseismic deformation fields were derived for these earthquakes using joint evaluation of near-field strong motion data, Global Navigation Satellite System data, and Synthetic Aperture Radar datasets. The coseismic slip distribution model was determined through the joint kinematic finite fault inversion. The Mw 7.9 earthquake was a left-lateral strike-slip event, predominantly occurring at depths up to 20 km. The earthquake displayed three distinct asperities that correlate well with bends and stepovers along the EAFZ. The Mw 7.7 earthquake also exhibited left-lateral strike-slip characteristics, with a major asperity along the Çardak Fault featuring a maximum slip of approximately 9.5 m at depths between 0 and 24 km. The occurrence of this unanticipated large Mw 7.9 catastrophic seismic event on a fault with low-intermediate structural maturity is noteworthy. In the vicinity of immature faults with multiple jogs, stress tends to accumulate at barrier locations. When the accumulated stress near several adjacent barriers reaches a certain threshold, it may result in the transformation of multiple barriers into asperities, triggering cascading ruptures.
Exercise ameliorates muscular excessive mitochondrial fission, insulin resistance and inflammation in diabetic rats via irisin/AMPK activation
Authors: Lin Junjie , Zhang Xin , Sun Yu , Xu Haocheng , Li Nan , Wang Yuanxin , Tian Xin , Zhao Chen , Wang Bin , Zhu Baishu , Zhao Renqing
Published: May 2024

This study aimed to investigate the effects of exercise on excessive mitochondrial fission, insulin resistance, and inflammation in the muscles of diabetic rats. The role of the irisin/AMPK pathway in regulating exercise effects was also determined. Thirty-two 8-week-old male Wistar rats were randomly divided into four groups (n = 8 per group): one control group (Con) and three experimental groups. Type 2 diabetes mellitus (T2DM) was induced in the experimental groups via a high-fat diet followed by a single intraperitoneal injection of streptozotocin (STZ) at a dosage of 30 mg/kg body weight. After T2DM induction, groups were assigned as sedentary (DM), subjected to 8 weeks of treadmill exercise training (Ex), or exercise training combined with 8-week cycloRGDyk treatment (ExRg). Upon completion of the last training session, all rats were euthanized and samples of fasting blood and soleus muscle were collected for analysis using ELISA, immunofluorescence, RT-qPCR, and Western blotting. Statistical differences between groups were analyzed using one-way ANOVA, and differences between two groups were assessed using t-tests. Our findings demonstrate that exercise training markedly ameliorated hyperglycaemia, hyperlipidaemia, and insulin resistance in diabetic rats (p < 0.05). It also mitigated the disarranged morphology and inflammation of skeletal muscle associated with T2DM (p < 0.05). Crucially, exercise training suppressed muscular excessive mitochondrial fission in the soleus muscle of diabetic rats (p < 0.05), and enhanced irisin and p-AMPK levels significantly (p < 0.05). However, exercise-induced irisin and p-AMPK expression were inhibited by cycloRGDyk treatment (p < 0.05). Furthermore, the administration of CycloRGDyk blocked the effects of exercise training in reducing excessive mitochondrial fission and inflammation in the soleus muscle of diabetic rats, as well as the positive effects of exercise training on improving hyperlipidemia and insulin sensitivity in diabetic rats (p < 0.05). These results indicate that regular exercise training effectively ameliorates insulin resistance and glucolipid metabolic dysfunction, and reduces inflammation in skeletal muscle. These benefits are partially mediated by reductions in mitochondrial fission through the irisin/AMPK signalling pathway.
Multi-condition simulation analysis of a sliding hydraulic support system based on elastoplastic mechanics
Authors: Wang Changji , Wang Wanting , Guo Yu , Zhao Dewang
Published: May 2024

In response to the challenges of supporting fractured and weak surrounding rock in deep coal mines in the Huainan region of China, a self-moving hydraulic support system for roof support was designed and developed. This innovative solution addresses the difficulties encountered in providing continuous support to roof structures. Based on the theory of elastoplastic mechanics, a numerical analysis model was established to calculate the mechanical parameters such as the displacement, stress, and strain of hydraulic supports during the stepping process under multiple operating conditions. The results of the numerical simulation were compared and verified with those from an actual working site. The results show that the maximum equivalent stress is 245.33 MPa for operating condition 1, 246.82 MPa for operating condition 2, and 245.27 MPa for operating condition 3. The maximum stress values under the three working conditions do not exceed the yield strength of the material, satisfying the requirements for normal bracket support operations. These research findings can establish a theoretical framework for the comprehensive assessment of the reliability and stability of hydraulic supports and the optimization of construction processes.
Differential expression analysis identifies a prognostically significant extracellular matrix–enriched gene signature in hyaluronan-positive clear cell renal cell carcinoma
Authors: Jokelainen Otto , Rintala Teemu J. , Fortino Vittorio , Pasonen-Seppänen Sanna , Sironen Reijo , Nykopp Timo K.
Published: May 2024

Hyaluronan (HA) accumulation in clear cell renal cell carcinoma (ccRCC) is associated with poor prognosis; however, its biology and role in tumorigenesis are unknown. RNA sequencing of 48 HA-positive and 48 HA-negative formalin-fixed paraffin-embedded (FFPE) samples was performed to identify differentially expressed genes (DEG). The DEGs were subjected to pathway and gene enrichment analyses. The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma (TCGA-KIRC) data and DEGs were used for the cluster analysis. In total, 129 DEGs were identified. HA-positive tumors exhibited enhanced expression of genes related to extracellular matrix (ECM) organization and ECM receptor interaction pathways. Gene set enrichment analysis showed that epithelial–mesenchymal transition-associated genes were highly enriched in the HA-positive phenotype. A protein–protein interaction network was constructed, and 17 hub genes were discovered. Heatmap analysis of TCGA-KIRC data identified two prognostic clusters corresponding to HA-positive and HA-negative phenotypes. These clusters were used to verify the expression levels and conduct survival analysis of the hub genes, 11 of which were linked to poor prognosis. These findings enhance our understanding of hyaluronan in ccRCC.