bluuebunny/crossref_metadata_2025 huggingface dataset:
Variability and trends of the tropical tropopause derived from a 1980–2021 multi-reanalysis assessment
Authors: Zou Ling , Hoffmann Lars , Müller Rolf , Spang Reinhold
Published: August 2023

As the tropopause plays a key role in regulating the entry of air from the troposphere into the stratosphere and in controlling stratosphere-troposphere exchange, variation of the tropopause impacts the atmospheric dynamics, circulation patterns, and the distribution of greenhouse gases in the upper troposphere and lower stratosphere (UTLS). Therefore, it is of particular interest to investigate the climatological characteristics and trends of the tropopause. Previous studies have investigated the tropopause characteristics using reanalyses and multi-source observations. This study extends the analysis of long-term variability and trends of tropical tropopause characteristics in earlier studies from 1980 up to 2021 using the modern ERA5 reanalysis and compares the results with those of other reanalyses, including ERA-Interim, MERRA-2, and NCEP1/2. Our analysis reveals a general rise and cooling of the tropical tropopause between 1980 and 2021. The geopotential height has increased by approximately 0.06 ± 0.01 km/decade (at a 95% confidence level), while the temperature has decreased by −0.09 ± 0.03 K/decade (at a 95% confidence level) for both the lapse rate tropopause and the cold point tropopause in ERA5. However, from 2006 to 2021, ERA5 shows a warming tropical tropopause (0.10 ± 0.11 K/decade) along with a slower rise in tropopause height (0.05 ± 0.02 km/decade) (at a 95% confidence level). Furthermore, our analysis demonstrates a decline in the rise and cooling of the tropical tropopause since the late 1990s, based on moving 20-year window trends in ERA5. Similar trends are observed in other investigated reanalyses. In addition, this study evaluated the variability of the width of the tropical belt based on tropopause height data from the reanalyses. The ERA5 data show a narrowing tropical belt (−0.16 ± 0.11°/decade) for the time period 1980–2021 according to the relative threshold method. It reveals a tropical widening (0.05 ± 0.22°/decade) for the period between 1980 and 2005, followed by a tropical narrowing (−0.17 ± 0.42°/decade) after 2006. However, the large uncertainties pose a challenge in drawing definitive conclusions on the change of tropical belt width. Despite the many challenges involved in deriving the characteristics and trends of the tropopause from reanalysis data, this study and the open reanalysistropopause data sets provided to the community will help to better inform future assessments of stratosphere-troposphere exchange and studies of chemistry and dynamics of the upper troposphere and lower stratosphere region.
Processing misconceptions: dynamic systems perspectives on thinking and learning
Authors: Svoboda Julia
Published: August 2023

The study of complex dynamic systems is central to biology. In this essay, I argue that thinking and learning can also be understood as phenomena that emerge from the continuous interactivity of dynamic systems. I first present and apply core concepts of dynamic systems theories to both biological and cognitive systems. I then use these ideas to explain how a dynamic systems perspective can recast the way we think about misconceptions, a central concept in the field of education research. Rather than model misconceptions as object-like entities that students either have or do not have, misconceptions can be modeled as patterns that emerge from continuous cognitive processes. I end by discussing how adopting a dynamic systems perspective suggests a need for research that uses methods designed to study processes in time and can inspire educators to embrace and value variation and fluctuation in students’ thinking and learning.
A three-month physical training program improves cardiovascular autonomic function in patients with metabolic syndrome with and without diabetes – a pilot study
Authors: Vágvölgyi Anna , Ábrahám Judit Erzsébet , Máthéné Köteles Éva , Korom Andrea , Barnai Mária , Szűcs Mónika , Orosz Andrea , Kempler Péter , Menyhárt Adrienn , Nemes Attila , Várkonyi Tamás , Baczkó István , Kósa István , Lengyel Csaba
Published: August 2023

Introduction

Vascular complications and neuropathy may develop in the presence of metabolic syndrome. The aim of our study was to measure the cardiovascular autonomic function following physical training in patients with metabolic syndrome with and without diabetes.

Subjects and methods

56 patients with metabolic syndrome (32 men/24 women, 40 non-diabetic patients (NDMetS)/16 diabetic patients (DMetS) [mean ± SD]: age: 50.35 ± 8.03 vs. 56.8 ± 9.30 years, p=0.023; baseline BMI: 32.2 ± 7.03 vs. 32.8 ± 5.94 kg/m2, p=0.739) were involved in our study. All tests and measurements were carried out before and following a 3-month physical training period. Autonomic function was assessed by means of five standard cardiovascular reflex tests. ECG repolarization parameters, including short-term QT variability and stress-ECG were also measured.

Results

In the whole population, Valsalva-ratio (VR) and the autonomic score (AS) improved following training (VR: 1.49 ± 0.24 vs. 1.64 ± 0.34, p=0.001; AS: 2.05 ± 1.73 vs. 1.41 ± 1.36, p=0.015) accompanied by the significant decrease of the systolic (150.3 ± 16.12 vs. 134.1 ± 16.67 mmHg, p<0.001) and diastolic (90.64 ± 12.8 vs. 82.79 ± 11.1 mmHg, p<0.001) blood pressure. An improvement in VR was detected in NDMetS patients following training (1.51 ± 0.24 vs. 1.67 ± 0.31, p= 0.002). No significant changes could be detected in autonomic tests’ results in the DMetS patient group following training. The applied exercise training program did not lead to significant changes in ECG repolarization. The stress-ECG test in the whole study population yielded a significant increase in the test duration (12.9 ± 3.76 vs. 15.1 ± 2.96 min, p<0.001) and in the test load (10.5 ± 2.78 vs. 11.6 ± 2.39 MET, p<0.001). The load capability improved significantly in both subgroups: 11.1 ± 2.04 vs. 12.1 ± 1.82, (p<0.001) and 9.0 ± 3.64 vs. 10.4 ± 3.05, (p=0.033) in subpopulations of NDMetS and DMetS, respectively. The DMetS patients achieved a significantly lower MET score at baseline (p=0.039) and following training (p=0.044) in comparison to the NDMetS patients.

Conclusion

The three-month exercise program improved the Valsalva-ratio and the AN score in the MetS patients, that is potentially protective against cardiovascular events. The training had some beneficial effect on blood pressure and the results of the stress-ECG tests in both groups. The absence of significant change in the reflex tests in DMetS group reflects an impaired adaptation compared to the NDMestS group.
Hormonal and non-hormonal oral contraceptives given long-term to pubertal rats differently affect bone mass, quality and metabolism
Authors: Porwal Konica , Sharma Shivani , Kumar Saroj , Tomar Manendra Singh , Sadhukhan Sreyanko , Rajput Swati , Kulkarni Chirag , Shrivastava Ashutosh , Kumar Navin , Chattopadhyay Naibedya
Published: August 2023

Introduction

We investigated the effects of hormonal and non-hormonal oral contraceptives (OCs) on bone mass, mineralization, composition, mechanical properties, and metabolites in pubertal female SD rats.

Methods

OCs were given for 3-, and 7 months at human equivalent doses. The combined hormonal contraceptive (CHC) was ethinyl estradiol and progestin, whereas the non-hormonal contraceptive (NHC) was ormeloxifene. MicroCT was used to assess bone microarchitecture and BMD. Bone formation and mineralization were assessed by static and dynamic histomorphometry. The 3-point bending test, nanoindentation, FTIR, and cyclic reference point indentation (cRPI) measured the changes in bone strength and material composition. Bone and serum metabolomes were studied to identify potential biomarkers of drug efficacy and safety and gain insight into the underlying mechanisms of action of the OCs.

Results

NHC increased bone mass in the femur metaphysis after 3 months, but the gain was lost after 7 months. After 7 months, both OCs decreased bone mass and deteriorated trabecular microarchitecture in the femur metaphysis and lumbar spine. Also, both OCs decreased the mineral: matrix ratio and increased the unmineralized matrix after 7 months. After 3 months, the OCs increased carbonate: phosphate and carbonate: amide I ratios, indicating a disordered hydroxyapatite crystal structure susceptible to resorption, but these changes mostly reversed after 7 months, indicating that the early changes contributed to demineralization at the later time. In the femur 3-point bending test, CHC reduced energy storage, resilience, and ultimate stress, indicating increased susceptibility to micro-damage and fracture, while NHC only decreased energy storage. In the cyclic loading test, both OCs decreased creep indentation distance, but CHC increased the average unloading slope, implying decreased microdamage risk and improved deformation resistance by the OCs. Thus, reduced bone mineralization by the OCs appears to affect bone mechanical properties under static loading, but not its cyclic loading ability. When compared to an age-matched control, after 7 months, CHC affected 24 metabolic pathways in bone and 9 in serum, whereas NHC altered 17 in bone and none in serum. 6 metabolites were common between the serum and bone of CHC rats, suggesting their potential as biomarkers of bone health in women taking CHC.

Conclusion

Both OCs have adverse effects on various skeletal parameters, with CHC having a greater negative impact on bone strength.
The effect of industrial structure adjustment and economic development quality on transitional China’s urban‒rural income inequity
Authors: Jiang Tianhe , Han Jun
Published: August 2023

Industrial structure adjustment is one of the key impetuses for China’s transition to high-quality economic development, influencing urban‒rural income equity through resource reallocation. This paper presents a theoretical analysis of the impact of industrial structure adjustment and economic development quality on the urban‒rural income gap. A spatial dynamic model is adopted to analyse the influence of the industrial structure adjustment range, adjustment quality and economic development quality on the urban‒rural income gap. The results based on full sample analysis suggest that the influence of the industrial structure adjustment range on the urban‒rural income gap is not significant, while the quality of industrial structure adjustment is significantly positively correlated with the urban‒rural income gap; the quality of economic development suppresses the income gap between urban and rural areas. The findings from regional analysis indicate that industrial structure adjustment and the quality of economic development have significantly heterogeneous effects in the eastern, central and western regions. Further analysis reveals threshold effects for both industrial structure adjustment and economic development quality on the urban‒rural income gap. Given this, policy implications for a fairer development transition are noted based on an interpretation of the empirical results.
Citizen science can add value to Phytophthora monitoring: five case studies from western North America
Authors: Lanning Kara K. , Kline Norma , Elliott Marianne , Stamm Elizabeth , Warnick Taylor , LeBoldus Jared M. , Garbelotto Matteo , Chastagner Gary , Hulbert Joseph M.
Published: August 2023

Phytophthora species are plant pathogens responsible for many notable biological invasions in agricultural, forests, and natural ecosystems. Detection and monitoring for invasive introductions of Phytophthora spp. is time and resource intensive. Development of citizen science detection and monitoring programs can aid in these efforts focused on reducing Phythophthora impacts. There are multiple methods for monitoring and detecting Phytophthora invasions suitable for citizen science approaches such as, leaf sampling, stream baiting or soil collections. Here we summarize five active projects in western North America where citizen scientists are aiding the monitoring and research efforts surrounding Phytophthora species and their impacts. Projects varied in scope, scale, methods, and capacity, but each project increased citizen scientists’ abilities for surveillance and advanced detection or knowledge of Phytophthora species. Some projects were integrated with school programs, others involved hands-on training with small groups, and another approach invited mass participation from interested citizens. Overall, all projects had positive outcomes multiplied across education, monitoring, and research. Together these case studies demonstrate how citizen scientists can amplify surveillance efforts, advance baseline knowledge, and reduce the impacts of biological invasions.
Multi-isotopic analysis reveals the early stem turtle Odontochelys as a nearshore herbivorous forager
Authors: Goedert Jean , Amiot Romain , Anquetin Jérémy , Séon Nicolas , Bourgeais Renaud , Bailly Gilles , Fourel François , Simon Laurent , Li Chun , Wang Wei , Lécuyer Christophe
Published: August 2023

Introduction

After decades of debate on the origin of turtles, it is now widely accepted that they are diapsid reptiles originating in the Permian from a terrestrial ancestor. It seems that the initial development of the structures that will later form the unique turtle bony shell took place as a response to a fossorial lifestyle. However, the earliest stem turtle with a fully complete plastron, Odontochelys semitestacea from the Late Triassic (lower Carnian) of China, is somewhat controversially interpreted as an aquatic or even a marine form, raising the question of the environment in which the completion of the plastron happened.

Methods

Here, we analyzed the stable carbon, oxygen and sulfur isotope compositions (δ13C, δ18O and δ34S) of bones from two specimens of Odontochelys along with bones and teeth of two associated specimens of the marine ichthyosaur Guizhouichthyosaurus tangae.

Results and discussion

We first show that δ18O values of Odontochelys are incompatible with a terrestrial lifestyle and imply a semi-aquatic to aquatic lifestyle. Isotopic results also demonstrate that the aquatic environment of Odontochelys was submitted to a strong marine influence, therefore excluding the possibility of a strict freshwater aquatic environment. Additionally, an unusual carbon isotope composition shows that O. semitestacea was herbivorous, probably consuming macrophytic algae in coastal zones like the extant green sea turtle (Chelonia mydas) or the marine iguana (Amblyrhynchus cristatus) do.
Patterns and consequences of invasion of tropical montane forests by Cestrum aurantiacum Lindl. in the Western Ghats
Authors: Das Arundhati A. , Ratnam Jayashree , Jathanna Devcharan
Published: August 2023

In the montane forest-grassland mosaics of the Western Ghats, land cover conversion to silviculture and agriculture over the last five decades has resulted in both loss of natural habitats and widespread invasion of remnant habitat patches. While invasion of the grassland habitats of the mosaic has been relatively well studied, there have been few attempts to understand the extent to which forest habitats (locally known as sholas) have been affected by the spread of exotic species. Here we examine the patterns and impacts of invasion of shola forest understoreys by Cestrum aurantiacum Lindl., an exotic shrub species. At the landscape scale, we demonstrate that the presence and abundance of this invasive in shola understories is negatively related to distance from tea plantations. Further, the intensity of invasion is higher in areas with greater seasonality of temperature and lower mean annual precipitation. At the patch scale, invasion is greatest at shola edges and away from stream courses. We find that C. aurantiacum abundance has negatively affected the regeneration of native shola tree species as well as the abundance of native shola understorey shrubs. Fifty three percent of invaded plots had no native shrubs present. In plots where both C. aurantiacum and native shrubs were present in large enough numbers, we found evidence of negative spatial dependence between stem locations of C. aurantiacum and native shrubs. Our findings have important implications for the management and conservation of these mosaics.
Going belowground: burying anthropomorphic biases on gustation and olfaction
Authors: Elmquist Dane C. , Eigenbrode Sanford D.
Published: August 2023

Chemical signaling underpins behavioral interactions among organisms in the soil. Understanding chemical communication in the soil requires a paradigm shift in methodology and perspectives compared to aboveground ecosystems because olfaction and gustation, accepted modalities of chemosensation aboveground, may not accurately represent chemical communication in the soil. To fully understand chemical communication in the soil, it is essential to consider how soil properties, such as moisture, pH, and adsorption, affect the transport and perception of semiochemicals. De-anthropomorphizing the study of chemosensation can avoid potential biases, particularly in soil systems, where distinctions between olfaction and gustation are confounded by the heterogeneity of the soil environment and its effects on the mobility of chemical signals. In this perspective, we first explore how soil heterogeneity confounds the dichotomy between olfaction and gustation with hypothetical but ecologically relevant examples. Then we examine how anthropomorphic biases in aboveground chemical ecology have influenced soil chemical ecology. Our examples and discussion are prepared primarily in reference to soil arthropods. We conclude by discussing seven future research directions and outstanding questions. The soil is a premier example of a system where investigators should avoid anthropomorphisms when studying behavioral and chemical ecology. Research in soil chemical ecology should further efforts towards developing a unified view of chemosensation that could apply to all environments where chemical communication occurs.
Landscape factors influencing bird nest site selection in urban green spaces
Authors: Ding Zhenhui , Guo Ao , Lian Meng , Wang Yan , Ying Wenjia , Jiang Hao , Zhou Xingyu , Qian Cheng , Lai Jiangshan , Cao Jiajie
Published: August 2023

Introduction

Understanding the birds’ breeding strategies in urban habitats is vital for ensuring their continued existence. Therefore, more research must be conducted on bird breeding and urban adaptation strategies in urban green spaces. This study aimed to address this gap by investigating the influence of landscape factors on the selection of bird nest sites.

Methods

Data on the presence and absence of magpie (Pica pica) and gray magpie (Cyanopica cyana) nests were collected through field surveys conducted in the campus of Nanjing Forestry University during the 2023 breeding season. Generalized additive models (GAMs) incorporating landscape variables were employed to assess the effects of these predictors on nest occurrence. The model with the lowest Akaike’s information criterion value was selected among the candidate GAMs.

Results

Below is a summary of the main results. Nest tree height (TH), distance from the central lawn (D), and tree coverage (TC) within the sampled area were identified as the primary landscape factors influencing nest site choice. Conversely, factors such as the shortest distance to the water source, herb coverage, shrub coverage, percentage of buildings, and percentage of hard pavement did not significantly impact on nest site selection. Furthermore, the nesting potential of magpies and grey magpies initially increased with tree height, reaching a maximum at ca. TH=25 meters after which it began to decline. The nesting occurrence rate showed an initial decrease tendency with increasing distance from the central lawn, reaching a minimum at D=400 meters, and then increased with further distance. Additionally, nesting potential decreased initially with increasing of TC in the range of 0–20%, fluctuated evenly between 20–60% TC, and decreased rapidly when TC exceeded 60%.

Discussion

This study provides valuable insights into the selection of nest sites by birds in urban habitats, specifically with respect to landscape factors. The understanding of the impact of urban green spaces on urban birds and the underlying mechanisms of their behavior contributes to the conservation of wild birds and promotes the harmonious development of urban areas.
Therapeutic potential of TNFR2 agonists: a mechanistic perspective
Authors: Chen Yibo , Jiang Mengmeng , Chen Xin
Published: August 2023

TNFR2 agonists have been investigated as potential therapies for inflammatory diseases due to their ability to activate and expand immunosuppressive CD4+Foxp3+ Treg cells and myeloid-derived suppressor cells (MDSCs). Despite TNFR2 being predominantly expressed in Treg cells at high levels, activated effector T cells also exhibit a certain degree of TNFR2 expression. Consequently, the role of TNFR2 signaling in coordinating immune or inflammatory responses under different pathological conditions is complex. In this review article, we analyze possible factors that may determine the therapeutic outcomes of TNFR2 agonism, including the levels of TNFR2 expression on different cell types, the biological properties of TNFR2 agonists, and disease status. Based on recent progress in the understanding of TNFR2 biology and the study of TNFR2 agonistic agents, we discuss the future direction of developing TNFR2 agonists as a therapeutic agents.
A novel cuproptosis pattern and tumor immune microenvironment characterization in urothelial carcinoma of the bladder
Authors: Feng Huan , Deng Zhiyao , Huang Yibao , Liu Zhuo , Ruan Yajun , Wang Tao , Liu Jihong
Published: August 2023

Background

Urothelial carcinoma of the bladder (UCB) is the most prevalent malignant tumor of the urinary system worldwide, which has a significant recurrence rate despite multiple treatment options available. As a unique and novel copper-dependent programmed cell death mechanism, the comprehensive impact of cuproptosis on the tumor immune microenvironment, clinicopathological characteristics and the prognosis of patients remains largely unclear.

Methods

A total of 568 UCB samples were thoroughly examined for cuproptosis patterns using data downloaded from TCGA and GEO, based on 10 cuproptosis-related genes reported previously. Then, the univariate COX regression analysis was performed on the genes that differed across the various patterns. To measure individual cuproptosis pattern, a cuproptosis score system was constructed using a principal component analysis algorithm. To validate the scoring system, immunohistochemical staining was performed on tumor tissues with different pathological grades, and experiments in vitro were conducted about the differentially expressed genes related to prognosis. Finally, the capacity of scoring system to predict the response to immunotherapy was verified by using data from IMvigor 210 cohort.

Results

Four unique cuproptosis clusters and two gene clusters were finally found by the investigation. The clinical features and prognosis of patients, as well as the mRNA transcriptome, pathway enrichment, and immune cell infiltration in TME, varied dramatically between various cuproptosis clusters and gene clusters. To identify individual cuproptosis patterns in UCB patients, we also established a cuproptosis scoring system. After validation with multiple methods, it was indicated that the score system could predict the prognosis of UCB patients and was significantly connected to clinical features such TNM category, tumor grade, molecular type and ultimate survival status. The clinical outcomes of UCB patients were predicted effectively according to the tumor mutation burden in conjunction with the scoring system. Furthermore, we found that the cuproptosis score had a significant correlation with the response to immunotherapy and the sensitivity to chemotherapy.

Conclusion

This study revealed the potential impact of cuproptosis on the UCB tumor immune microenvironment and clinical pathological characteristics. The cuproptosis score system could effectively predict the prognosis of patients and the response to chemotherapy and immunotherapy.
Human T cells loaded with superparamagnetic iron oxide nanoparticles retain antigen-specific TCR functionality
Authors: Pfister Felix , Dörrie Jan , Schaft Niels , Buchele Vera , Unterweger Harald , Carnell Lucas R. , Schreier Patrick , Stein Rene , Kubánková Markéta , Guck Jochen , Hackstein Holger , Alexiou Christoph , Janko Christina
Published: August 2023

Background

Immunotherapy of cancer is an emerging field with the potential to improve long-term survival. Thus far, adoptive transfer of tumor-specific T cells represents an effective treatment option for tumors of the hematological system such as lymphoma, leukemia or myeloma. However, in solid tumors, treatment efficacy is low owing to the immunosuppressive microenvironment, on-target/off-tumor toxicity, limited extravasation out of the blood vessel, or ineffective trafficking of T cells into the tumor region. Superparamagnetic iron oxide nanoparticles (SPIONs) can make cells magnetically controllable for the site-specific enrichment.

Methods

In this study, we investigated the influence of SPION-loading on primary human T cells for the magnetically targeted adoptive T cell therapy. For this, we analyzed cellular mechanics and the T cell response after stimulation via an exogenous T cell receptor (TCR) specific for the melanoma antigen MelanA or the endogenous TCR specific for the cytomegalovirus antigen pp65 and compared them to T cells that had not received SPIONs.

Results

SPION-loading of human T cells showed no influence on cellular mechanics, therefore retaining their ability to deform to external pressure. Additionally, SPION-loading did not impair the T cell proliferation, expression of activation markers, cytokine secretion, and tumor cell killing after antigen-specific activation mediated by the TCR.

Conclusion

In summary, we demonstrated that SPION-loading of T cells did not affect cellular mechanics or the functionality of the endogenous or an exogenous TCR, which allows future approaches using SPIONs for the magnetically enrichment of T cells in solid tumors.
Context matters when using climate model projections for aquaculture
Authors: Falconer Lynne , Ytteborg Elisabeth , Goris Nadine , Lauvset Siv K. , Sandø Anne Britt , Hjøllo Solfrid Sætre
Published: August 2023

At present, specific guidance on how to choose, assess and interpret climate model projections for the aquaculture sector is scarce. Since many aspects of aquaculture production are influenced by the local farm-level environment, there is a need to consider how climate model projections can be used to predict potential future farming conditions locally. This study comparedin-situmeasurements of temperature and salinity from Norwegian salmon farms and fixed monitoring stations to simulations from a regional ocean climate model for multiple locations and depths in southern Norway. For locations considered in this study, a similar seasonal cycle in terms of phasing was visible for modelled and measured temperatures. For some depths and times of the year the modelled and measured temperatures were similar, but for others there were differences. The model tended to underestimate temperature. On occasion there were differences between average modelled and measured temperatures of several degrees and aquaculture users would need to consider the implications of using the modelled temperatures. As for salinity, the model does not include localized freshwater inputs, so the model overestimated salinity for locations close to shore and was not able to represent more brackish water conditions in shallower depths. It was not possible to draw a general conclusion as to whether the model was suitable for aquaculture purposes, as the similarities and differences between the modelled and measured values varied by variable, area, depth, and time. These findings made it clear that aquaculture users would have to implement a process to determine whether they could use climate model outputs for their specific purpose. A model vetting framework is presented that can be used to support decisions on the use of climate model projections for aquaculture purposes. The vetting framework describes four stages that can be used to establish the necessary context regarding the aquaculture requirements and model capabilities, and then check how the model is simulating the conditions of interest at farm sites. Although the focus was aquaculture, the findings are relevant for other sectors and the framework can guide use of climate models for more local-scale assessment and management in coastal locations.
Ocean mixing during Hurricane Ida (2021): the impact of a freshwater barrier layer
Authors: Miles Travis N. , Coakley Samuel J. , Engdahl Julia M. , Rudzin Johna E. , Tsei Senam , Glenn Scott M.
Published: August 2023

Tropical cyclones are one of the costliest and deadliest natural disasters globally, and impacts are currently expected to worsen with a changing climate. Hurricane Ida (2021) made landfall as a category 4 storm on the US Gulf coast after intensifying over a Loop Current eddy and a freshwater barrier layer. This freshwater layer extended from the coast to the open ocean waters south of the shelf-break of the northern Gulf of Mexico (GoM). An autonomous underwater glider sampled this ocean feature ahead of Hurricane Ida operated through a partnership between NOAA, Navy, and academic institutions. In this study we evaluate hurricane upper ocean metrics ahead of and during the storm as well as carry out 1-D shear driven mixed layer model simulations to investigate the sensitivity of the upper ocean mixing to a barrier layer during Ida’s intensification period. In our simulations we find that the freshwater barrier layer inhibited cooling by as much as 57% and resulted in enhanced enthalpy flux to the atmosphere by as much as 11% and an increase in dynamic potential intensity (DPI) of 5 m s-1 (~9.72 knots) in the 16 hours leading up to landfall. This highlights the utility of new ocean observing systems in identifying localized ocean features that may impact storm intensity ahead of landfall. It also emphasizes the northern Gulf of Mexico and the associated Mississippi River plume as a region and feature where the details of upper ocean metrics need to be carefully considered ahead of landfalling storms.
Thermal conductivity performance in sodium alginate-based Casson nanofluid flow by a curved Riga surface
Authors: Nagaraja K. V. , Vinutha K. , Madhukesh J. K. , Khan Umair , Singh Chohan Jasgurpreet , Sherif El-Sayed M. , Sarris Ioannis E. , Hassan Ahmed M. , Shanker B.
Published: August 2023

This study examines the effects of a porous media and thermal radiation on Casson-based nano liquid movement over a curved extending surface. The governing equations are simplified into a system of ODEs (ordinary differential equations) using the appropriate similarity variables. The numerical outcomes are obtained using the shooting method and Runge-Kutta Fehlbergs fourth-fifth order (RKF-45). An analysis is conducted to discuss the impact of significant nondimensional constraints on the thermal and velocity profiles. The findings show that the rise in curvature constraint will improve the velocity but diminish the temperature. The increased values of the modified Hartmann number raise the velocity, but a reverse trend is seen for increased porosity parameter values. Thermal radiation raises the temperature, while modified Hartmann numbers and the Casson factor lower the velocity but raise the thermal profile. Moreover, the existence of porous and solid fractions minimizes the surface drag force, and radiation and solid fraction components enhance the rate of thermal dispersion. The findings of this research may have potential applications in the design of heat exchangers used in cooling electronic devices like CPUs and GPUs, as well as microscale engines such as microturbines and micro-heat engines.
No causal association between plasma cystatin C and cardiovascular diseases: Mendelian randomization analyses in UK biobank
Authors: Tu Jingjing , Xu Ying , Guo Xu , Zhang Jiayu , Xu Duo , Han Liyuan , Wang Yue , Zhang Boya , Sun Hongpeng
Published: August 2023

Background

We aimed to determine whether the plasma cystatin C is a causal risk factor for cardiovascular events, stroke, myocardial infarction (MI), and cardiovascular disease (CVD) mortality by conducting Mendelian randomization (MR) designs.

Methods

Our study included 277,057 individuals free of CVDs or cancer at baseline in the UK Biobank. The genetic scores of plasma cystatin C comprising 67 single-nucleotide polymorphisms were calculated on the basis of data from a large genome-wide association study. By stratifying the genetic score, we conducted cox regression to assess the relationship between plasma cystatin C and CVDs. In this study, linear MR analysis was used to estimate the causal association between plasma cystatin C and CVDs.

Results

Observational analyses showed that plasma cystatin C concentrations were associated with the risk of CVDs [hazard ratios (HR) per standard deviation (SD) 1.09, 95% confidence interval (CI); 1.07–1.10] and CVD mortality (1.14, 1.11–1.17). Among CVDs, plasma cystatin C were associated with stroke (1.10, 1.08–1.11) and MI (1.08, 1.07–1.10). Linear MR analysis did not provide evidence of a causal association between plasma cystatin C and the risk of CVDs [odds ratio (OR) per SD 0.96, 95% CI;0.90–1.03], stroke (0.96, 0.93–1.01), MI (0.97, 0.91–1.03), and CVD mortality (0.98, 0.96–1.01), with consistent estimates from sensitivity analyses.

Conclusion

Observational findings indicated that higher plasma cystatin C is associated with a higher risk of CVDs; According to MR studies, there is no causal association between plasma cystatin C and the risk of CVDs and CVD mortality.
Burden of disease and impact on quality of life in chronic back pain – a comparative cross-sectional study of 150 axial spondyloarthritis and 150 orthopedic back pain patients
Authors: Frede Natalie , Hiestand Sonja , Endres Dominique , van Elst Ludger Tebartz , Finzel Stephanie , Chevalier Nina , Schramm Markus A. , Rump Ina C. , Thiel Jens , Voll Reinhard , Herget Georg , Venhoff Nils
Published: August 2023

Objective

Chronic back pain (CBP) constitutes one of the most common complaints in primary care and a leading cause of disability worldwide. CBP may be of mechanical or inflammatory character and may lead to functional impairment and reduced quality of life. In this study, we aimed to assess and compare burden of disease, functional capacity, quality of life and depressive symptoms in axial spondyloarthritis (axSpA) patients with orthopedic chronic back pain patients (OBP). We further aimed to identify factors associated with quality of life.

Methods

Cross-sectional survey of a cohort of 300 CBP patients including 150 patients from a University Hospital Orthopedic Back Pain Outpatient Clinic with OBP and 150 patients with confirmed axSpA from a University Hospital Rheumatology Outpatient Clinic. Questionnaire-based assessment of pain character (Inflammatory Back Pain, MAIL-Scale), functional status (FFbH, BASFI), quality of life (WHOQOL-Bref) and depressive symptoms (Phq9) and retrospective medical chart analysis.

Results

Both, OBP and axSpA patients reported on average intermediate pain levels of mostly mixed pain character. Both groups demonstrated a reduced health-related quality of life and the presence of depressive symptoms. However, axSpA patients reported a significantly better subjective quality of life, more satisfaction with their health status and better functional capacity compared to OBP patients (all p < 0.001). In a multivariate regression model, depressive symptoms, mechanical back pain, pain level and age were negative predictors of subjective quality of life, whereas functional capacity was a positive predictor.

Conclusion

Chronic back pain was associated with a high morbidity and reduced quality of life regardless of pain character. We identified multiple factors associated with reduced quality of life. Awareness and addressing of these factors may help to overcome unmet needs and improve quality of life for these patients.
Remote patient monitoring strategies and wearable technology in chronic obstructive pulmonary disease
Authors: Coutu Felix-Antoine , Iorio Olivia C. , Ross Bryan A.
Published: August 2023

Chronic obstructive pulmonary disease (COPD) is highly prevalent and is associated with a heavy burden on patients and health systems alike. Exacerbations of COPD (ECOPDs) are a leading cause of acute hospitalization among all adult chronic diseases. There is currently a paradigm shift in the way that ECOPDs are conceptualized. For the first time, objective physiological parameters are being used to define/classify what an ECOPD is (including heart rate, respiratory rate, and oxygen saturation criteria) and therefore a mechanism to monitor and measure their changes, particularly in an outpatient ambulatory setting, are now of great value. In addition to pre-existing challenges on traditional ‘in-person’ health models such as geography and seasonal (ex. winter) impacts on the ability to deliver in-person visit-based care, the COVID-19 pandemic imposed additional stressors including lockdowns, social distancing, and the closure of pulmonary function labs. These health system stressors, combined with the new conceptualization of ECOPDs, rapid advances in sophistication of hardware and software, and a general openness by stakeholders to embrace this technology, have all influenced the propulsion of remote patient monitoring (RPM) and wearable technology in the modern care of COPD. The present article reviews the use of RPM and wearable technology in COPD. Context on the influences, factors and forces which have helped shape this health system innovation is provided. A focused summary of the literature of RPM in COPD is presented. Finally, the practical and ethical principles which must guide the transition of RPM in COPD into real-world clinical use are reviewed.
Nicotinamide enhances myelin production after demyelination through reduction of astrogliosis and microgliosis
Authors: Kaplanis Stefanos Ioannis , Kaffe Despoina , Ktena Niki , Lygeraki Andriani , Kolliniati Ourania , Savvaki Maria , Karagogeos Domna
Published: August 2023

Caloric restriction is the chronic reduction of total caloric intake without malnutrition and has attracted a lot of attention as, among multiple other effects, it attenuates demyelination and stimulates remyelination. In this study we have evaluated the effect of nicotinamide (NAM), a well-known caloric restriction mimetic, on myelin production upon demyelinating conditions. NAM is the derivative of nicotinic acid (vitamin B3) and a precursor of nicotinamide adenine dinucleotide (NAD+), a ubiquitous metabolic cofactor. Here, we use cortical slices ex vivo subjected to demyelination or cultured upon normal conditions, a lysolecithin (LPC)-induced focal demyelination mouse model as well as primary glial cultures. Our data show that NAM enhances both myelination and remyelination ex vivo, while it also induces myelin production after LPC-induced focal demyelination ex vivo and in vivo. The increased myelin production is accompanied by reduction in both astrogliosis and microgliosis in vivo. There is no direct effect of NAM on the oligodendrocyte lineage, as no differences are observed in oligodendrocyte precursor cell proliferation or differentiation or in the number of mature oligodendrocytes. On the other hand, NAM affects both microglia and astrocytes as it decreases the population of M1-activated microglia, while reducing the pro-inflammatory phenotype of astrocytes as assayed by the reduction of TNF-α. Overall, we show that the increased myelin production that follows NAM treatment in vivo is accompanied by a decrease in both astrocyte and microglia accumulation at the lesion site. Our data indicate that NAM influences astrocytes and microglia directly, in favor of the remyelination process by promoting a less inflammatory environment.
Nourishing the brain on deep space missions: nutritional psychiatry in promoting resilience
Authors: Pathare Nihar N. , Fayet-Moore Flavia , Fogarty Jennifer A. , Jacka Felice N. , Strandwitz Philip , Strangman Gary E. , Donoviel Dorit B.
Published: August 2023

The grueling psychological demands of a journey into deep space coupled with ever-increasing distances away from home pose a unique problem: how can we best take advantage of the benefits of fresh foods in a place that has none? Here, we consider the biggest challenges associated with our current spaceflight food system, highlight the importance of supporting optimal brain health on missions into deep space, and discuss evidence about food components that impact brain health. We propose a future food system that leverages the gut microbiota that can be individually tailored to best support the brain and mental health of crews on deep space long-duration missions. Working toward this goal, we will also be making investments in sustainable means to nourish the crew that remains here on spaceship Earth.
Linking neural circuits to the mechanics of animal behavior in Drosophila larval locomotion
Authors: Kohsaka Hiroshi
Published: August 2023

The motions that make up animal behavior arise from the interplay between neural circuits and the mechanical parts of the body. Therefore, in order to comprehend the operational mechanisms governing behavior, it is essential to examine not only the underlying neural network but also the mechanical characteristics of the animal’s body. The locomotor system of fly larvae serves as an ideal model for pursuing this integrative approach. By virtue of diverse investigation methods encompassing connectomics analysis and quantification of locomotion kinematics, research on larval locomotion has shed light on the underlying mechanisms of animal behavior. These studies have elucidated the roles of interneurons in coordinating muscle activities within and between segments, as well as the neural circuits responsible for exploration. This review aims to provide an overview of recent research on the neuromechanics of animal locomotion in fly larvae. We also briefly review interspecific diversity in fly larval locomotion and explore the latest advancements in soft robots inspired by larval locomotion. The integrative analysis of animal behavior using fly larvae could establish a practical framework for scrutinizing the behavior of other animal species.
Identification of immune-related biomarkers co-occurring in acute ischemic stroke and acute myocardial infarction
Authors: Wang Shan , Tan Shengjun , Chen Fangni , An Yihua
Published: August 2023

Background

Acute ischemic stroke (AIS) and acute myocardial infarction (AMI) share several features on multiple levels. These two events may occur in conjunction or in rapid succession, and the occurrence of one event may increase the risk of the other. Owing to their similar pathophysiologies, we aimed to identify immune-related biomarkers common to AIS and AMI as potential therapeutic targets.

Methods

We identified differentially expressed genes (DEGs) between the AIS and control groups, as well as AMI and control groups using microarray data (GSE16561 and GSE123342). A weighted gene co-expression network analysis (WGCNA) approach was used to identify hub genes associated with AIS and/or AMI progression. The intersection of the four gene sets identified key genes, which were subjected to functional enrichment and protein–protein interaction (PPI) network analyses. We confirmed the expression levels of hub genes using two sets of gene expression profiles (GSE58294 and GSE66360), and the ability of the genes to distinguish patients with AIS and/or AMI from control patients was assessed by calculating the receiver operating characteristic values. Finally, the investigation of transcription factor (TF)-, miRNA-, and drug–gene interactions led to the discovery of therapeutic candidates.

Results

We identified 477 and 440 DEGs between the AIS and control groups and between the AMI and control groups, respectively. Using WGCNA, 2,776 and 2,811 genes in the key modules were identified for AIS and AMI, respectively. Sixty key genes were obtained from the intersection of the four gene sets, which were used to identify the 10 hub genes with the highest connection scores through PPI network analysis. Functional enrichment analysis revealed that the key genes were primarily involved in immunity-related processes. Finally, the upregulation of five hub genes was confirmed using two other datasets, and immune infiltration analysis revealed their correlation with certain immune cells. Regulatory network analyses indicated that GATA2 and hsa-mir-27a-3p might be important regulators of these genes.

Conclusion

Using comprehensive bioinformatics analyses, we identified five immune-related biomarkers that significantly contributed to the pathophysiological mechanisms of both AIS and AMI. These biomarkers can be used to monitor and prevent AIS after AMI, or vice versa.
A network meta-analysis of therapeutic and prophylactic management of vasospasm on aneurysmal subarachnoid hemorrhage outcomes
Authors: Chousterman Benjamin , Leclère Brice , Morisson Louis , Eude Yannick , Gayat Etienne , Mebazaa Alexandre , Cinotti Raphael
Published: August 2023

Background

Vasospasm and cerebral ischemia after aneurysmal subarachnoid hemorrhage are associated with mortality and poor neurological outcomes. We studied the efficacy of all available strategies targeting vasospasm and cerebral ischemia on outcomes in a network meta-analysis.

Methods

We searched EMBASE and MEDLINE databases from 1 January 1990 and 28 November 2021 according to PRISMA guidelines. Randomized controlled trials and longitudinal studies were included. All curative or preventive strategies targeting vasospasm and/or cerebral ischemia were eligible. A network meta-analysis was performed to compare all interventions with one another in a primary (randomized controlled trials only) and a secondary analysis (both trials and longitudinal studies). Mortality by 3 months was the primary outcome. Secondary outcomes were vasospasm, neurological outcome by 3 months, and dichotomized as “good” or “poor” recovery according to each study definition.

Results

A total of 2,382 studies were screened which resulted in the selection of 192 clinical trials (92 (47.9%) and 100 cohorts (52.1%) and the inclusion of 41,299 patients. In randomized controlled studies, no strategy decreased mortality by 3 months. Statins (0.79 [0.62–1]), tirilazad (0.82 [0.69–0.97]), CSF drainage (0.47 [0.29–0.77]), and clazosentan (0.51 [0.36–0.71]) significantly decreased the incidence of vasospasm. Cilostazol was the only treatment associated with improved neurological outcomes by 3 months in the primary (OR 1.16, 95% CI [1.05–1.28]) and secondary analyses (OR 2.97, 95% CI [1.39–6.32]).

Discussion

In the modern era of subarachnoid hemorrhage, all strategies targeting vasospasm failed to decrease mortality. Cilostazol should be confirmed as a treatment to improve neurological outcomes. The link between vasospasm and neurological outcome appears questionable.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=116073, identifier: PROSPERO CRD42018116073.