bluuebunny/crossref_metadata_2025 huggingface dataset:
A putative glucose 6-phosphate isomerase has pleiotropic functions on virulence and other mechanisms in Acidovorax citrulli
Authors: Heo Lynn , Han Yoobin , Cho Yongmin , Choi Junhyeok , Lee Jeongwook , Han Sang-Wook
Published: November 2023

Acidovorax citrulli (Ac) is a causal agent of watermelon bacterial fruit blotch (BFB) disease. Because resistance cultivars/lines have not yet been developed, it is imperative to elucidate Ac’s virulence factors and their mechanisms to develop resistant cultivars/lines in different crops, including watermelon. The glucose-6-phosphate isomerase (GPI) is a reversible enzyme in both glycolysis and gluconeogenesis pathways in living organisms. However, the functions of GPI are not characterized in Ac. In this study, we determined the roles of GpiAc (GPI in Ac) by proteomic and phenotypic analyses of the mutant lacking GPI. The mutant displayed significantly reduced virulence to watermelon in two different virulence assays. The mutant’s growth patterns were comparable to the wild-type strain in rich medium and M9 with glucose but not with fructose. The comparative proteome analysis markedly identified proteins related to virulence, motility, and cell wall/membrane/envelope. In the mutant, biofilm formation and twitching halo production were reduced. We further demonstrated that the mutant was less tolerant to osmotic stress and lysozyme treatment than the wild-type strain. Interestingly, the tolerance to alkali conditions was remarkably enhanced in the mutant. These results reveal that GpiAc is involved not only in virulence and glycolysis/gluconeogenesis but also in biofilm formation, twitching motility, and tolerance to diverse external stresses suggesting the pleiotropic roles of GpiAc in Ac. Our study provides fundamental and valuable information on the functions of previously uncharacterized glucose 6-phosphate isomerase and its virulence mechanism in Ac.
Transitioning from junior to senior: a case study on elite judokas in South Korea
Authors: Hong Hee Jung , Hong Seung Han
Published: November 2023

Introduction

This study explores the experiences of Korean elite judokas during their junior to senior transition (JST), including both male and female participants, to provide empirical evidence for the development of tailored support services or programs for this target population.

Methods

We recruited 12 elite judokas for our study, comprising eight males and four females, all in their first year of university. Given their preferences and availability at the time of data collection, participants were divided into three focus groups: Focus Group 1 (FG1; four male participants), Focus Group 2 (FG2; four female participants), and Focus Group 3 (FG3; four male participants). Thematic analysis was applied to analyze the data from the focus group interviews.

Results

Five main themes were identified: (a) COVID-19-Induced Frustration, (b) From Big Fish in a Small Pond to Small Fish in a Big Pond, (c) Challenges in Academic Commitment, (d) Adapting to Transitions in Living Arrangements, and (e) Recognizing Support Needs for the JST.

Discussion

The findings of this study provide both theoretical and practical implications that could improve judokas’ experiences during the challenging physical and mental phase of JST, as well as inform the establishment of tailored support programs and schemes for successful and smooth JSTs for athletes.
Detecting and Processing Unsuspected Sensitive Variables for Robust Machine Learning
Authors: Risser Laurent , Picard Agustin Martin , Hervier Lucas , Loubes Jean-Michel
Published: November 2023

The problem of algorithmic bias in machine learning has recently gained a lot of attention due to its potentially strong impact on our societies. In much the same manner, algorithmic biases can alter industrial and safety-critical machine learning applications, where high-dimensional inputs are used. This issue has, however, been mostly left out of the spotlight in the machine learning literature. Contrary to societal applications, where a set of potentially sensitive variables, such as gender or race, can be defined by common sense or by regulations to draw attention to potential risks, the sensitive variables are often unsuspected in industrial and safety-critical applications. In addition, these unsuspected sensitive variables may be indirectly represented as a latent feature of the input data. For instance, the predictions of an image classifier may be altered by reconstruction artefacts in a small subset of the training images. This raises serious and well-founded concerns about the commercial deployment of AI-based solutions, especially in a context where new regulations address bias issues in AI. The purpose of our paper is, then, to first give a large overview of recent advances in robust machine learning. Then, we propose a new procedure to detect and to treat such unknown biases. As far as we know, no equivalent procedure has been proposed in the literature so far. The procedure is also generic enough to be used in a wide variety of industrial contexts. Its relevance is demonstrated on a set of satellite images used to train a classifier. In this illustration, our technique detects that a subset of the training images has reconstruction faults, leading to systematic prediction errors that would have been unsuspected using conventional cross-validation techniques.
A Bibliometric Analysis and Systematic Review of Dark Tourism: Trends, Impact, and Prospects
Authors: Mora Forero Jorge Alexander , Nieto Mejia Alvelayis , León-Gómez Ana
Published: November 2023

The main objective of this bibliometric review is to analyse and synthesise the evolution of the field of black tourism through an extensive analysis of the scientific literature. To this end, the article carries out a bibliometric analysis and systematic review that will provide information on the most studied topics, the most influential authors and publications, and the areas that require further research. The findings and conclusions of this study represent a valuable contribution to the literature on dark tourism by providing an intrinsic and comprehensive analysis of the field’s current state. The analysis provides a better understanding of the theoretical and conceptual framework of the articles published to date, which is essential to lay the groundwork for future research and enrich the academic debate on dark tourism.
Modification of Space Debris Trajectories through Lasers: Dependence of Thermal and Impulse Coupling on Material and Surface Properties
Authors: Keil Denise , Scharring Stefan , Klein Erik , Lorbeer Raoul-Amadeus , Schumacher Dennis , Seiz Frederic , Sharma Kush Kumar , Zwilich Michael , Schnörer Lukas , Roth Markus , Ben-Larbi Mohamed Khalil , Wiedemann Carsten , Riede Wolfgang , Dekorsy Thomas
Published: November 2023

Environmental pollution exists not only within our atmosphere but also in space. Space debris is a critical problem of modern and future space infrastructure. Congested orbits raise the question of spacecraft disposal. Therefore, state-of-the-art satellites come with a deorbit system in cases of low Earth orbit (LEO) and with thrusters for transferring into the graveyard orbit for geostationary and geosynchronous orbits. No practical solution is available for debris objects that stem from fragmentation events. The present study focuses on objects in LEO orbits with dimensions in the dangerous class of 1 to 10 cm. Our assumed method for the change of trajectories of space debris is laser ablation for collision avoidance or complete removal by ground-based laser systems. Thus, we executed an experimental feasibility study with focus on thermal and impulse coupling between laser and sample. Free-fall experiments with a 10 ns laser pulse at nominally 60 J and 1064 nm were conducted with GSI Darmstadt’s nhelix laser on various sample materials with different surfaces. Ablated mass, heating, and trajectory were recorded. Furthermore, we investigated the influence of the sample surface roughness on the laser-object interaction. We measured impulse coupling coefficients between 7 and 40 µNs/J and thermal coupling coefficients between 2% and 12.5% both depending on target fluence, surface roughness, and material. Ablated mass and changes in surface roughness were considered via simulation to discriminate their relevance for a multiple shot concept.
Effect of Two Biostimulants, Based on Ascophyllum nodosum Extracts, on Strawberry Performance under Mild Drought Stress
Authors: Shakya Rashmi , Capilla Elvira , Torres-Pagán Natalia , Muñoz Marta , Boscaiu Monica , Lupuţ Ioan , Vicente Oscar , Verdeguer Mercedes
Published: November 2023

The world’s population continues to grow while available natural resources, such as arable land, water, and quality soil, are decreasing. Therefore, it is essential to implement environmentally friendly crop management strategies, which include the use of biostimulants. This study analysed the effects on strawberry plants of ActyseiTM and Phylgreen®, two commercial biostimulants based on extracts of the seaweed Ascophyllum nodosum. The study was conducted under field capacity (regular irrigation) and at 50% field capacity (mild water stress conditions) for 12 weeks. Different growth parameters of the aerial parts of the plants were measured weekly, such as the number of leaves, length of the longest leaf, leaf area, and the number of flowers and fruits produced, as well as the chlorophyll content, determined with a single-photon avalanche diode (SPAD) detector. At the end of the experiment, the plant material was collected, and the roots and aerial parts were weighed separately to obtain the fresh and dry weight of the samples. Fruit quality was assessed by analysing morphological parameters (weight and size) and some biochemical variables (proline, total soluble sugars, and antioxidant compounds contents). ActyseiTM application generally enhanced plant growth in control plants and under mild water stress conditions, even though root weight was reduced. In contrast, no significant effect of Phylgreen® on vegetative growth was observed, except for stimulating the root growth of plants watered at field capacity. Both biostimulants, Phylgreen® to a greater extent, showed an impact on the plants already seven weeks after their initial application, stimulating flower and fruit production, especially at field capacity.
Impact of Agricultural Drought on Barley and Wheat Yield: A Comparative Case Study of Spain and Germany
Authors: Benito-Verdugo Pilar , Martínez-Fernández José , González-Zamora Ángel , Almendra-Martín Laura , Gaona Jaime , Herrero-Jiménez Carlos Miguel
Published: November 2023

Given the growing interest in drought impacts on crops, this work studied the impact of agricultural drought on wheat and barley during the period 2001–2020. The study was carried out in the Spanish regions of Castilla y León and Castilla–La Mancha, with approximate areas of 94,000 km2 and 79,000 km2, respectively, and in the German regions of Nordrhein-Westfalen, Niedersachsen and Bayern, with approximate areas of 34,000 km2, 48,000 km2 and 71,000 km2, respectively. These are the main cereal-growing regions of Spain and Germany. Soil moisture (SM) in the root zone was extracted from the LISFLOOD model database, and SM anomalies were used as the agricultural drought index. Gross primary productivity (GPP) and leaf area index (LAI) variables were obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS), and the month in which SM is most influential on these crop state variables was identified. Crop yields in Spain and Germany were obtained from the Spanish Ministry of Agriculture, Fisheries and Food and the German Federal Statistical Office, respectively. Agricultural drought years and their impact on cereal yields were determined on a regional scale using three approaches based on the critical month with different time periods. These approaches were the use of the critical month and the two (before or after) and the three months (before and after) around the critical month. Two different analyses were used to identify the critical month, depending on the different environmental conditions in each country. These two approaches consisted of a monthly correlation analysis between SM anomalies and cereal yield in Spain and a monthly trend analysis of SM anomalies in Germany. The results showed a dependence of crop variables on SM in spring months in both countries and in summer months in Germany. Differences were found depending on the environmental conditions. A considerable reduction in cereal yields was obtained in Spain which exceeded 30%. Similarly, a worrying sign was observed in Germany, with a positive agricultural drought trend and a yield reduction of almost 5% in cereal crops. In view of future forecasts of the negative impact of climate change on global food production, this study provides valuable information for water and agricultural management under climate change scenarios. Both in regions that are already threatened and in those that until recently were not affected, it is necessary to study adaptation measures to avoid aggravating the impact of agricultural drought on crops, which could improve water productivity and future food security.
Optimal Coverage Path Planning for Agricultural Vehicles with Curvature Constraints
Authors: Höffmann Maria , Patel Shruti , Büskens Christof
Published: November 2023

Complete coverage path planning (CCPP) is vital in mobile robot applications. Optimizing CCPP is particularly significant in precision agriculture, where it enhances resource utilization, reduces soil compaction, and boosts crop yields. This work offers a comprehensive approach to CCPP for agricultural vehicles with curvature constraints. Our methodology comprises four key stages. First, it decomposes complex agricultural areas into simpler cells, each equipped with guidance tracks, forming a fixed track system. The subsequent route planning and smooth path planning stages compute a path that adheres to path constraints, optimally traverses the cells, and aligns with the track system. We use the generalized traveling salesman problem (GTSP) to determine the optimal traversing sequence. Additionally, we introduce an algorithm for calculating paths that are both smooth and curvature-constrained within individual cells, as well as paths that enable seamless transitions between cells, resulting in a smooth, curvature-constraint coverage path. Our modular approach allows method flexibility at each step. We evaluate our method on real agricultural fields, demonstrating its effectiveness in minimizing path length, ensuring efficient coverage, and adhering to curvature constraints. This work establishes a strong foundation for precise and efficient agricultural coverage path planning, with potential for further real-world applications and enhancements.
Mapping Subchondral Bone Density Distribution in the Canine C6-C7 Vertebral Endplates: A CT-OAM Study
Authors: Kramer Vincenz , Böttcher Peter
Published: November 2023

Intervertebral cage subsidence is a common complication in treating disc-associated cervical spondylomyelopathy in dogs. The mechanical stability of the vertebral endplate in contact with the cage is crucial to preventing subsidence. This study aims to assess subchondral bone mineral density (sBMD) in the canine vertebral endplate (specifically, the C6-C7 vertebral motion unit) as a measure of its mechanical stability. The sBMD distribution was mapped for the C6 caudal and C7 cranial vertebral endplates in 15 middle- to large-breed dogs using computed tomography osteoabsorptiometry. The sBMD distribution in the canine C6 and C7 vertebral endplates exhibited a heterogeneous pattern, with lower density observed in the central and dorsal contact areas of the nucleus pulposus, where common subsidence occurs. Our results suggest a potential need to redesign intervertebral cages to ensure that contact areas align with regions of higher bone density. A broad-based design extending toward the lateral and dorsal aspects of the annulus fibrosus contact area may enhance stability.
Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle
Authors: He Guangxia , Chen Chao , Mei Shihui , Chen Ze , Zhang Rong , Zhang Tiantian , Xu Duhan , Zhu Mingming , Luo Xiaofen , Zeng Chengrong , Zhou Bijun , Wang Kaigong , Zhu Erpeng , Cheng Zhentao
Published: November 2023

Fermented distillers’ grains (FDG) are commonly used to enhance the health and metabolic processes of livestock and poultry by regulating the composition and activity of the intestinal microbiota. Nevertheless, there is a scarcity of research on the effects of the FDG diet on the gastrointestinal microbiota and its metabolites in cattle. This study examines the impact of FDG dietary supplements on the gastrointestinal flora and metabolic profile of Guanling cattle. Eighteen cattle were randomly assigned to three treatment groups with six replicates per group. The treatments included a basal diet (BD), a 15% concentrate replaced by FDG (15% FDG) in the basal diet, and a 30% concentrate replaced by FDG (30% FDG) in the basal diet. Each group was fed for a duration of 60 days. At the conclusion of the experimental period, three cattle were randomly chosen from each group for slaughter and the microbial community structure and metabolic mapping of their abomasal and cecal contents were analyzed, utilizing 16S rDNA sequencing and LC-MS technology, respectively. At the phylum level, there was a significant increase in Bacteroidetes in both the abomasum and cecum for the 30%FDG group (p < 0.05). Additionally, there was a significant reduction in potential pathogenic bacteria such as Spirochetes and Proteobacteria for both the 15%FDG and 30%FDG groups (p < 0.05). At the genus level, there was a significant increase (p < 0.05) in Ruminococcaceae_UCG-010, Prevotellaceae_UCG-001, and Ruminococcaceae_UCG-005 fiber degradation bacteria. Non-target metabolomics analysis indicated that the FDG diet significantly impacted primary bile acid biosynthesis, bile secretion, choline metabolism in cancer, and other metabolic pathways (p < 0.05). There is a noteworthy correlation between the diverse bacterial genera and metabolites found in the abomasal and cecal contents of Guanling cattle, as demonstrated by correlation analysis. In conclusion, our findings suggest that partially substituting FDG for conventional feed leads to beneficial effects on both the structure of the gastrointestinal microbial community and the metabolism of its contents in Guanling cattle. These findings offer a scientific point of reference for the further use of FDG as a cattle feed resource.
The Effects of Stocking Density and Food Deprivation on Mucous Cells and Lysozyme Activity in the Skin and Gills of Silver Catfish
Authors: Scherer Gabriela Pires , Zavaglia Isadora Maria Oliveira , Sutili Fernando Jonas , Silva Hugo Napoleão Pereira , Furquim Magale Dallaporta , da Veiga Marcelo Leite , Baldisserotto Bernardo , da Costa Sílvio Teixeira
Published: November 2023

This study aimed to identify the regions of the body surface of silver catfish (Rhamdia quelen) with a higher population of mucous cells in the skin. Additionally, the effects of stressful conditions, such as different stocking densities and food deprivation, on the proliferative response of mucous cells in the skin and gill epithelium and their impact on cutaneous mucous lysozyme activity were investigated. Silver catfish were divided into four experimental groups: high stocking density (32 kg/m3) and fed (HSD-F), high stocking density and fasted (HSD-FS), low stocking density (2.5 kg/m3) and fed (LSD-F), and low stocking density and fasted (LSD-FS). Fish in the fed groups received commercial feed twice a day, amounting to 1% of the tank biomass. After a 14-day experimental period, the fish were anesthetized and euthanized. Samples of cutaneous mucous and skin fragments from seven different points and the second left branchial arch were collected. Histological slides of the skin and gills were stained with PAS + Alcian Blue at pH 2.5, and the epidermal mucous lysozyme activity was assessed using the turbidimetric method. The ventral point in front of the ventral fin was found to be the optimal location for collecting cutaneous epithelia due to its higher density of mucous cells. The population of mucous cells in both the skin and gills varied based on the collection point and treatment applied. The highest lysozyme activity in the epidermal mucous was observed in fish from the HSD-F group. Overall, these findings suggest that stocking density and food deprivation create stressful conditions for silver catfish, which modulate their mucosal response to each situation.
Genotyping-by-Sequencing Strategy for Integrating Genomic Structure, Diversity and Performance of Various Japanese Quail (Coturnix japonica) Breeds
Authors: Volkova Natalia A. , Romanov Michael N. , Abdelmanova Alexandra S. , Larionova Polina V. , German Nadezhda Yu. , Vetokh Anastasia N. , Shakhin Alexey V. , Volkova Ludmila A. , Anshakov Dmitry V. , Fisinin Vladimir I. , Narushin Valeriy G. , Griffin Darren K. , Sölkner Johann , Brem Gottfried , McEwan John C. , Brauning Rudiger , Zinovieva Natalia A.
Published: November 2023

Traces of long-term artificial selection can be detected in genomes of domesticated birds via whole-genome screening using single-nucleotide polymorphism (SNP) markers. This study thus examined putative genomic regions under selection that are relevant to the development history, divergence and phylogeny among Japanese quails of various breeds and utility types. We sampled 99 birds from eight breeds (11% of the global gene pool) of egg (Japanese, English White, English Black, Tuxedo and Manchurian Golden), meat (Texas White and Pharaoh) and dual-purpose (Estonian) types. The genotyping-by-sequencing analysis was performed for the first time in domestic quails, providing 62,935 SNPs. Using principal component analysis, Neighbor-Net and Admixture algorithms, the studied breeds were characterized according to their genomic architecture, ancestry and direction of selective breeding. Japanese and Pharaoh breeds had the smallest number and length of homozygous segments indicating a lower selective pressure. Tuxedo and Texas White breeds showed the highest values of these indicators and genomic inbreeding suggesting a greater homozygosity. We revealed evidence for the integration of genomic and performance data, and our findings are applicable for elucidating the history of creation and genomic variability in quail breeds that, in turn, will be useful for future breeding improvement strategies.
Morphological and Ontogenetic Skin Color Changes in the American Alligator (Alligator mississippiensis)
Authors: Francis Cadre , Hale Amber , Berken Jennifer , Joanen Ted , Merchant Mark
Published: November 2023

To assess skin color change in alligators, we maintained animals in differently lighted environments and also measured skin colors in an ontogenetic series of wild animals. Juvenile alligators maintained in black enclosures exhibited a gradual lightening of skin color when shifted to white enclosures, and these observed changes were reversible. A histological examination of the skins of alligators maintained in dark tanks showed that the dermis exhibited a dense layer of pigmented cells, while samples from the same animals in light environments exhibited a more diffuse pigmented layer. As alligators grow, they exhibit an ontogenetic loss of stripes that may aid in crypsis and predation. Hatchlings have intense black and yellow vertical stripes that darken with age; adults are a more homogenous black/gray color. Since alligators live in temperate climates and adults have lower surface area/volume ratios, which can be detrimental for the absorption of radiant energy, the darker color of larger animals may also aid in thermoregulation. Alligators at the northern end of their range, with colder climates, exhibited darker skin tones, and the ontogenetic extinction of stripes occurred at a more accelerated rate compared to animals in southern, warmer regions, supporting the idea that latitude-dependent ontogenetic color shift has a role in thermoregulation.
Social Trend Mining: Lead or Lag
Authors: Hassani Hossein , Komendantova Nadejda , Rovenskaya Elena , Yeganegi Mohammad Reza
Published: November 2023

This research underscores the profound implications of Social Intelligence Mining, notably employing open access data and Google Search engine data for trend discernment. Utilizing advanced analytical methodologies, including wavelet coherence analysis and phase difference, hidden relationships and patterns within social data were revealed. These techniques furnish an enriched comprehension of social phenomena dynamics, bolstering decision-making processes. The study’s versatility extends across myriad domains, offering insights into public sentiment and the foresight for strategic approaches. The findings suggest immense potential in Social Intelligence Mining to influence strategies, foster innovation, and add value across diverse sectors.
Development of a Screening Method for Fluoroquinolones in Meat Samples Using Molecularly Imprinted Carbon Dots
Authors: Hakiem Ahmed Faried Abdel , Urriza-Arsuaga Idoia , Urraca Javier L.
Published: November 2023

An accurate and simple screening method has been developed for the determination of fluoroquinolone antibiotics. Carbon dots were synthesized by simple hydrothermal treatment as highly fluorescent nano-sensors. They were subsequently used in the synthesis of organic-based molecularly imprinted polymers to develop fluorescence-based polymeric composites using enoxacin as a representative dummy template molecule of fluoroquinolones. The method was optimized concerning the pH of the medium and composite concentration. The normalized fluorescence intensity showed efficient quenching under optimized conditions upon successive addition of the template, with an excellent correlation coefficient. The proposed method was applied to eight other fluoroquinolones, exhibiting, in all cases, good correlation coefficients (0.65–0.992) within the same linearity range (0.03–2.60 mg mL−1). Excellent detection and quantification limits were been obtained for the target analytes down to 0.062 and 0.186 mg L−1, respectively. All studied analytes showed no interference with enrofloxacin, the most commonly used veterinary fluoroquinolone, with a percentage of cross-reactivity varying from 89.00 to 540.00%. This method was applied successfully for the determination of enrofloxacin in three different types of meat samples: beef, pork, and chicken, with good recoveries varying from 70 to 100% at three levels. This new procedure is an easy analytical method that can be useful as a screening method for monitoring the environmental hazard of fluoroquinolones in quality control laboratories.
Optical Diffraction Tomography and Raman Confocal Microscopy for the Investigation of Vacuoles Associated with Cancer Senescent Engulfing Cells
Authors: Ghislanzoni Silvia , Kang Jeon Woong , Bresci Arianna , Masella Andrea , Kobayashi-Kirschvink Koseki J. , Polli Dario , Bongarzone Italia , So Peter T. C.
Published: November 2023

Wild-type p53 cancer therapy-induced senescent cells frequently engulf and degrade neighboring ones inside a massive vacuole in their cytoplasm. After clearance of the internalized cell, the vacuole persists, seemingly empty, for several hours. Despite large vacuoles being associated with cell death, this process is known to confer a survival advantage to cancer engulfing cells, leading to therapy resistance and tumor relapse. Previous attempts to resolve the vacuolar structure and visualize their content using dyes were unsatisfying for lack of known targets and ineffective dye penetration and/or retention. Here, we overcame this problem by applying optical diffraction tomography and Raman spectroscopy to MCF7 doxorubicin-induced engulfing cells. We demonstrated a real ability of cell tomography and Raman to phenotype complex microstructures, such as cell-in-cells and vacuoles, and detect chemical species in extremely low concentrations within live cells in a completely label-free fashion. We show that vacuoles had a density indistinguishable to the medium, but were not empty, instead contained diluted cell-derived macromolecules, and we could discern vacuoles from medium and cells using their Raman fingerprint. Our approach is useful for the noninvasive investigation of senescent engulfing (and other peculiar) cells in unperturbed conditions, crucial for a better understanding of complex biological processes.
Organic Electronics in Biosensing: A Promising Frontier for Medical and Environmental Applications
Authors: Kaushal Jyoti Bala , Raut Pratima , Kumar Sanjay
Published: November 2023

The promising field of organic electronics has ushered in a new era of biosensing technology, thus offering a promising frontier for applications in both medical diagnostics and environmental monitoring. This review paper provides a comprehensive overview of organic electronics’ remarkable progress and potential in biosensing applications. It explores the multifaceted aspects of organic materials and devices, thereby highlighting their unique advantages, such as flexibility, biocompatibility, and low-cost fabrication. The paper delves into the diverse range of biosensors enabled by organic electronics, including electrochemical, optical, piezoelectric, and thermal sensors, thus showcasing their versatility in detecting biomolecules, pathogens, and environmental pollutants. Furthermore, integrating organic biosensors into wearable devices and the Internet of Things (IoT) ecosystem is discussed, wherein they offer real-time, remote, and personalized monitoring solutions. The review also addresses the current challenges and future prospects of organic biosensing, thus emphasizing the potential for breakthroughs in personalized medicine, environmental sustainability, and the advancement of human health and well-being.
Nicotine, THC, and Dolutegravir Modulate E-Cigarette-Induced Changes in Addiction- and Inflammation-Associated Genes in Rat Brains and Astrocytes
Authors: Kulbe Jacqueline Renee , Nguyen Lauren , Le Alexandra Anh , Laird Anna Elizabeth , Taffe Michael A. , Nguyen Jacques D. , Fields Jerel Adam
Published: November 2023

E-cigarette use has been marketed as a safer alternative to traditional cigarettes, as a means of smoking cessation, and are used at a higher rate than the general population in people with HIV (PWH). Early growth receptor 2 (EGR2) and Activity-Regulated Cytoskeleton-Associated Protein (ARC) have a role in addiction, synaptic plasticity, inflammation, and neurodegeneration. This study showed that 10 days of exposure to e-cigarette vapor altered gene expression in the brains of 6-month-old, male, Sprague Dawley rats. Specifically, the e-cigarette solvent vapor propylene glycol (PG) downregulated EGR2 and ARC mRNA expression in frontal cortex, an effect which was reversed by nicotine (NIC) and THC, suggesting that PG could have a protective role against NIC and cannabis dependence. However, in vitro, PG upregulated EGR2 and ARC mRNA expression at 18 h in cultured C6 rat astrocytes suggesting that PG may have neuroinflammatory effects. PG-induced upregulation of EGR2 and ARC mRNA was reversed by NIC but not THC. The HIV antiretroviral DTG reversed the effect NIC had on decreasing PG-induced upregulation of EGR2, which is concerning because EGR2 has been implicated in HIV latency reversal, T-cell apoptosis, and neuroinflammation, a process that underlies the development of HIV-associated neurocognitive disorders.
Mental Pain Correlates with Mind Wandering, Self-Reflection, and Insight in Individuals with Psychotic Disorders: A Cross-Sectional Study
Authors: Rodolico Alessandro , Cutrufelli Pierfelice , Brondino Natascia , Caponnetto Pasquale , Catania Gaetano , Concerto Carmen , Fusar-Poli Laura , Mineo Ludovico , Sturiale Serena , Signorelli Maria Salvina , Petralia Antonino
Published: November 2023

Understanding the cognitive processes that contribute to mental pain in individuals with psychotic disorders is important for refining therapeutic strategies and improving patient outcomes. This study investigated the potential relationship between mental pain, mind wandering, and self-reflection and insight in individuals diagnosed with psychotic disorders. We included individuals diagnosed with a ‘schizophrenia spectrum disorder’ according to DSM-5 criteria. Patients in the study were between 18 and 65 years old, clinically stable, and able to provide informed consent. A total of 34 participants, comprising 25 males and 9 females with an average age of 41.5 years (SD 11.5) were evaluated. The Psychache Scale (PAS), the Mind Wandering Deliberate and Spontaneous Scale (MWDS), and the Self-Reflection and Insight Scale (SRIS) were administered. Statistical analyses involved Spearman’s rho correlations, controlled for potential confounders with partial correlations, and mediation and moderation analyses to understand the indirect effects of MWDS and SRIS on PAS and their potential interplay. Key findings revealed direct correlations between PAS and MWDS and inverse correlations between PAS and SRIS. The mediation effects on the relationship between the predictors and PAS ranged from 9.22% to 49.8%. The largest statistically significant mediation effect was observed with the SRIS-I subscale, suggesting that the self-reflection and insight component may play a role in the impact of mind wandering on mental pain. No evidence was found to suggest that any of the variables could function as relationship moderators for PAS. The results underscore the likely benefits of interventions aimed at reducing mind wandering and enhancing self-reflection in psychotic patients (e.g., metacognitive therapy, mindfulness). Further research will be essential to elucidate the underlying mechanisms.
Hippocampal Resting State Functional Connectivity Associated with Physical Activity in Periadolescent Children
Authors: Heller-Wight Abi , Phipps Connor , Sexton Jennifer , Ramirez Meghan , Warren David E.
Published: November 2023

Periadolescence is a neurodevelopmental period characterized by structural and functional brain changes that are associated with cognitive maturation. The development of the functional connectivity of the hippocampus contributes to cognitive maturation, especially memory processes. Notably, hippocampal development is influenced by lifestyle factors, including physical activity. Physical activity has been associated with individual variability in hippocampal functional connectivity. However, this relationship has not been characterized in a developmental cohort. In this study, we aimed to fill this gap by investigating the relationship between physical activity and the functional connectivity of the hippocampus in a cohort of periadolescents aged 8–13 years (N = 117). The participants completed a physical activity questionnaire, reporting the number of days per week they performed 60 min of physical activity; then, they completed a resting-state functional MRI scan. We observed that greater physical activity was significantly associated with differences in hippocampal functional connectivity in frontal and temporal regions. Greater physical activity was associated with decreased connectivity between the hippocampus and the right superior frontal gyrus and increased connectivity between the hippocampus and the left superior temporal sulcus. Capturing changes in hippocampal functional connectivity during key developmental periods may elucidate how lifestyle factors including physical activity influence brain network connectivity trajectories, cognitive development, and future disease risk.
Implications of Social Anxiety Symptoms in Adults with Autism Spectrum Disorder: Is There a Predictive Role of Interpersonal Sensitivity and Substance Abuse?
Authors: Carpita Barbara , Cremone Ivan Mirko , Nardi Benedetta , Amatori Giulia , Bonelli Chiara , Massimetti Enrico , Casagrande Danila , Pini Stefano , Dell’Osso Liliana
Published: November 2023

Social anxiety disorder (SAD) has been frequently reported by subjects with Autism Spectrum Disorder (ASD). However, interestingly, the overlap between social anxiety and autistic traits may sometimes impede ASD diagnosis in subjects without intellectual or language impairment. The aim of the present work was to evaluate the presence and correlates of social phobic features among subjects with ASD, with a specific focus on evaluating which social anxiety symptoms may be statistically predictive of an ASD diagnosis. With this purpose, 48 subjects with ASD and 48 gender- and age- matched healthy controls (HCs) were recruited and assessed with the SHY-SV and the AdAS Spectrum questionnaires. Results highlighted higher scores in all SHY-SV Spectrum domains and total scores for the ASD group. Moreover, AdAS Spectrum scores were significantly correlated with all SHY-SV domain and total scores. A logistic regression analysis highlighted the SHY-SV Interpersonal sensitivity and Substance Abuse domains scores as significant positive predictors of an ASD diagnosis. These results confirm the link between ASD and SAD. Because of this association, particular attention should be paid to subjects with high interpersonal sensitivity traits and substance abuse problems.
Cashew (Anacardium occidentale) Extract: Possible Effects on Hypothalamic–Pituitary–Adrenal (HPA) Axis in Modulating Chronic Stress
Authors: Désiré Guedang Nyayi Simon , Simplice Foyet Harquin , Guillaume Camdi Woumitna , Kamal Fatima Zahra , Parfait Bouvourné , Hermann Tchinda Defo Serge , Hervé Ngatanko Abaissou Hervé , Eglantine Keugong Wado , Linda Damo Kamda Jorelle , Roland Rebe Nhouma , Balbine Kamleu Nkwingwa , Blondelle Kenko Djoumessi Lea , Ciobica Alin , Romila Laura
Published: November 2023

Depression presents a significant global health burden, necessitating the search for effective and safe treatments. This investigation aims to assess the antidepressant effect of the hydroethanolic extract of Anacardium occidentale (AO) on depression-related behaviors in rats. The depression model involved 42 days of unpredictable chronic mild stress (UCMS) exposure and was assessed using the sucrose preference and the forced swimming (FST) test. Additionally, memory-related aspects were examined using the tests Y-maze and Morris water maze (MWM), following 21 days of treatment with varying doses of the AO extract (150, 300, and 450 mg/kg) and Imipramine (20 mg/kg), commencing on day 21. The monoamines (norepinephrine, serotonin, and dopamine), oxidative stress markers (MDA and SOD), and cytokines levels (IL-1β, IL-6, and TNF-α) within the brain were evaluated. Additionally, the concentration of blood corticosterone was measured. Treatment with AO significantly alleviated UCMS-induced and depressive-like behaviors in rats. This was evidenced by the ability of the extract to prevent further decreases in body mass, increase sucrose consumption, reduce immobility time in the test Forced Swimming, improve cognitive performance in both tests Y-maze and the Morris water maze by increasing the target quadrant dwelling time and spontaneous alternation percentage, and promote faster feeding behavior in the novelty-suppressed feeding test. It also decreased pro-inflammatory cytokines, corticosterone, and MDA levels, and increased monoamine levels and SOD activity. HPLC-MS analysis revealed the presence of triterpenoid compounds (ursolic acid, oleanolic acid, and lupane) and polyphenols (catechin quercetin and kaempferol). These results evidenced the antidepressant effects of the AO, which might involve corticosterone and monoaminergic regulation as antioxidant and anti-inflammatory activities.
Analysis of the Operational Outcomes of an Energy-Sharing System for Low-Carbon Energy Community in South Korea
Authors: Eum Jiyoung , Lee Hansol , Choi Gyeong-Seok
Published: November 2023

The transition to a net-zero energy system is being promoted in the energy sector, which has led to the creation of energy prosumers. These produce, consume, and trade energy using renewable energy systems installed in buildings or complexes. Here, a community was set as the target to apply the concept of an energy prosumer at the individual building and regional levels. Energy-sharing systems were divided into three categories: energy production, energy storage, and energy management. Energy-sharing systems centered on electrical energy—photovoltaic, battery energy storage, and energy management systems—were installed in two communities located in South Korea, and the energy-sharing effects of the system operation were reported. Monthly power consumption in spring and fall exhibited significant savings of approximately three times that of winter consumption, owing to the energy-sharing systems. Daily hourly power-consumption patterns differed on weekdays and weekends because of the weekday working and building-use hours of the communities. Energy could be shared between communities and buildings because of surplus energy. More surplus power was available for energy sharing on weekends because power consumption was lower. Because energy trading and sharing are restricted, the related laws are being revised. Therefore, a low-carbon community can be realized through surplus energy trading and sharing technology between communities and buildings as renewable energy systems spread owing to low carbonization.
In Situ X-ray Diffraction as a Basic Tool to Study Oxide and Metal Oxide Catalysts
Authors: Bulavchenko Olga A. , Vinokurov Zakhar S.
Published: November 2023

X-ray diffraction (XRD) is a standard technique that is widely applied in heterogeneous catalysis to determine phase composition, atomic structure, and size of crystallites. This review is focused on the application of in situ XRD for studying the catalysts during their “lifetime” (under synthesis, activation, operation, and deactivation conditions), limiting the objects of research to oxide and metal oxide catalysts. Also included is a brief overview of modern techniques and instruments and the latest works illustrating different aspects of this technique in catalyst research. The main conclusion is that the field of heterogeneous catalysis research would benefit substantially from the application of in situ XRD for the structural, phase, and morphological characterization of solid catalysts. Even more useful information can be obtained if XRD is combined with other techniques that are more sensitive at length scales different from that of XRD.
Catalytic CO2 Methanation Reactors and Processes
Authors: Ngo Son Ich , García-Bordejé Enrique
Published: November 2023

CO2 methanation is a chemical process that involves the conversion of carbon dioxide (CO2) and hydrogen (H2) gases into methane (CH4) and water (H2O) [...]
Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
Authors: Tachibana Ryota , Takeuchi Hiroki , Yoshikawa-Terada Kento , Maezawa Tadashi , Nishioka Mikiko , Takayama Erina , Tanaka Hiroaki , Tanaka Kayo , Hyon Suong-hyu , Gen Yuki , Kondo Eiji , Ikeda Tomoaki
Published: November 2023

Sperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced SDF after freeze-thawing compared with clinically popular cryopreservation reagents containing human serum albumin. However, it is unclear whether CPLL reduces SDF, as it differed from the constituents of the commercial cryopreservation reagents used for comparison. Therefore, here, we examined whether CPLL reduces the SDF of human sperm and evaluated reactive oxygen species (ROS) levels and lipid peroxidation (LPO), which are the causes of SDF; mitochondrial injury, ROS production; and impaired sperm motility. Furthermore, optimal antioxidants and their concentrations that could further enhance the reduction in SDF were determined for future clinical application in ART and underwent the same functional evaluations. CPLL can reduce SDF via inhibition of intracytoplasmic ROS and LPO. Furthermore, the addition of 0.1 mM resveratrol avoided the enhancement of SDF, which potentially affects mitochondrial and cytoplasmic ROS and LPO. This novel human sperm cryopreservation reagent containing CPLL and resveratrol has the potential to improve treatment outcomes in ART using frozen sperm.
Electrodeposition of Iron Selenide: A Review
Authors: Piperno Laura , Celentano Giuseppe , Sotgiu Giovanni
Published: November 2023

In recent years, metal selenide materials have attracted attention due to their wide application prospects. In this family of materials, FeSe is particularly studied since it is both a semiconductor used in solar cells and a superconductor with a critical transition temperature, Tc, of 8 K. For any envisaged application, the possibility of preparing large-area FeSe thin films at low cost is extremely appealing, and one possible technique suitable for this purpose is electrodeposition. Several groups have reported successful electrodeposition of FeSe, but the investigated systems are different in many aspects, and the results are difficult to compare. The aim of this review is to collect the available information on FeSe electrodeposition and the thermodynamic laws controlling this process; to catalog the literature pointing out the differences in the experimental procedure and how they influence the results; and to draw general conclusions, if any, on this topic.
Analysis of Surface and Physicochemical Properties of Novel Hydrogel Materials Supported with Magnetic Nanoparticles
Authors: Sala Katarzyna , Cholewa Krzysztof , Bańkosz Magdalena , Tyliszczak Bożena
Published: November 2023

Nanotechnology is a field of science that has been growing rapidly in recent times. The use of this science in medicine makes it possible to develop new innovative therapies and materials with therapeutic effects. The topic of controlled delivery of therapeutic substances using appropriate carriers is extremely important. Such carriers can be, among others, magnetic nanoparticles. In the present study, magnetic nanoparticles coated with nanosilver were obtained. This carrier was then placed in a hydrogel matrix. The study shows that the properties of the obtained materials indicate their high application potential as transdermal systems. In this work, hydrogel materials modified with magnetic nanoparticles with silver were subjected to a physicochemical analysis. The sorption capacity of these materials was determined, and they were subjected to infrared spectroscopy analysis and incubation tests in simulated body fluids. In addition, the surface of these materials was characterized in detail. The results indicated that all the materials exhibit excellent sorption capacities, and their surfaces are characterized with high roughness.