bluuebunny/crossref_metadata_2025 huggingface dataset:
An Online Training Intervention on Prehospital Stroke Codes in Catalonia to Improve the Knowledge, Pre-Notification Compliance and Time Performance of Emergency Medical Services Professionals
Authors: Gorchs-Molist Montse , Solà-Muñoz Silvia , Enjo-Perez Iago , Querol-Gil Marisol , Carrera-Giraldo David , Nicolàs-Arfelis Jose María , Jiménez-Fàbrega Francesc Xavier , Pérez de la Ossa Natalia
Published: August 2020

Strokes are a time-dependent medical emergency. The training of emergency medical service (EMS) professionals is essential to ensure the activation of stroke codes with pre-notification, as well as a rapid transfer to achieve early therapy. New assessment scales for the detection of patients with suspected large vessel occlusion ensures earlier access to endovascular therapy. The aim of this study was to evaluate the impact on an online training intervention focused on the Rapid Arterial oCclusion Evaluation (RACE) scoring of EMS professionals based on the prehospital stroke code in Catalonia from 2014 to 2018 in a pre–post intervention study. All Catalonian EMS professionals and the clinical records from primary stroke patients were included. The Kirkpatrick model guided the evaluation of the intervention. Data were collected on the knowledge on stroke recognition and management, pre-notification compliance, activated stroke codes and time performance of EMS professionals. Knowledge improved significatively in most items and across all categories, reaching a global achievement of 82%. Pre-notification compliance also improved significantly and remained high in the long-term. Increasingly higher notification of RACE scores were recorded from 60% at baseline to 96.3% in 2018, and increased on-site clinical care time and global time were also observed. Therefore, the online training intervention was effective for increasing EMS professionals’ knowledge and pre-notification compliance upon stroke code activation, and the wide adoption of a new prehospital scale for the assessment of stroke severity (i.e., the RACE scale) was achieved.
What Is the Best Practice Method for Quantifying the Health and Economic Benefits of Active Transport?
Authors: Möller Holger , Haigh Fiona , Hayek Rema , Veerman Lennert
Published: August 2020

The aim of this study was to identify a best practice method to cost the health benefits of active transport for use in infrastructure planning in New South Wales, Australia. We systematically reviewed the international literature covering the concept areas of active transport and cost and health benefits. Original publications describing a method to cost the health benefits of active transport, published in 2000–2019 were included. Studies meeting the inclusion criteria were assessed against criteria identified in interviews with key government stakeholders. A total of 2993 studies were identified, 53 were assessed for eligibility, and 19 were included in the review. The most commonly studied active transport modes were cycling (n = 8) and walking and cycling (n = 6). Exposures considered were physical activity, road transport related injuries and air pollution. The most often applied economic evaluation method was cost benefit analysis (n = 8), and costs were commonly calculated by monetising health outcomes. Based on evaluation of models against the criteria, a Multistate Life Table model was recommended as the best method currently available. There is strong and increasing interest in quantifying and costing the health benefits of active transport internationally. Incorporating health-related economic benefits into existing regulatory processes such as cost benefit analyses could provide an effective way to encourage the non-health sector to include health impacts in infrastructure measures.
Dark Triad Traits and Risky Behaviours: Identifying Risk Profiles from a Person-Centred Approach
Authors: Maneiro Lorena , Navas María Patricia , Van Geel Mitch , Cutrín Olalla , Vedder Paul
Published: August 2020

The relationship between Dark Triad traits and risky behaviours has been shown in recent years. However, few studies have attempted to disentangle this relationship using a person-centred approach. The goal of the current study was to identify subgroups of individuals on the basis of their scores on Machiavellianism, psychopathy, and narcissism and analyse the differences between them in a set of risky behaviours (i.e., frequency of substance use, reactive and proactive aggression, risk perception and risk engagement, and problematic internet use). The sample consisted of 317 undergraduates aged 18–34 (46% males). The results of the latent profile analysis showed five subgroups of individuals that were identified based on their scores on the Dark Triad traits: low-Dark Triad, narcissistic, Machiavellian/narcissistic, psychopathic, and Machiavellian/psychopathic. Overall, the Machiavellian/narcissistic and Machiavellian/psychopathic subgroups showed higher scores for most risky behaviours. The low-Dark Triad scored higher for risk perception. No significant differences between subgroups were found as regards frequency of alcohol, tobacco, and cannabis use. These findings suggest that the combination of the Dark Triad traits lead to more negative outcomes as regards risky behaviour than individual components. Moreover, they highlight the relevance of using a person-centred approach in the study of dark personalities.
Emotional and Cognitive Responses and Behavioral Coping of Chinese Medical Workers and General Population during the Pandemic of COVID-19
Authors: Cai Zemin , Zheng Shukai , Huang Yanhong , Zhang Xuanzhi , Qiu Zhaolong , Huang Anyan , Wu Kusheng
Published: August 2020

Background: The outbreak of Corona Virus Disease 2019 (COVID-19) might affect the psychological health of population, especially medical workers. We aimed to investigate the impact of the COVID-19 pandemic on emotional and cognitive responses and behavioral coping among Chinese residents. Methods: An online investigation was run from 5 February to 25 February 2020, which recruited a total of 616 Chinese residents. Self-designed questionnaires were used to collect demographic information, epidemic knowledge and prevention of COVID-19 and characteristics of medical workers. The emotional and cognitive responses were assessed via the Symptom Check List-30 (SCL-30) and Yale–Brown Obsessive Compulsive Scale (Y-BOCS). Behavioral coping was assessed via Simplified Coping Style Questionnaire (SCSQ). Results: In total, 131 (21.3%) medical workers and 485 (78.7%) members of the general public completed the structured online survey. The structural equation models showed that emotional response interacted with cognitive response, and both emotional response and cognitive response affected the behavioral coping. Multivariate regression showed that positive coping enhanced emotional and cognitive responses, while negative coping reduced emotional and cognitive responses. The emotional response (depression, anxiety and photic anxiety) scores of the participants were higher than the norm (all p < 0.001); in particular, the panic scores of members of the general public were higher than those of medical workers (p < 0.05), as well as the cognitive response (paranoia and compulsion). Both positive and negative coping scores of the participants were lower than the norm (p < 0.001), and the general public had higher negative coping than medical workers (p < 0.05). Conclusion: During the preliminary stage of COVID-19, our study confirmed the significance of emotional and cognitive responses, which were associated with behavioral coping and significantly influenced the medical workers and the general public’s cognition and level of public health emergency preparedness. These results emphasize the importance of psychological health at times of widespread crisis.
Efficacy of a Multi-component m-Health Weight-loss Intervention in Overweight and Obese Adults: A Randomised Controlled Trial
Authors: Duncan Mitch , Fenton Sasha , Brown Wendy , Collins Clare , Glozier Nicholas , Kolt Gregory , Holliday Elizabeth , Morgan Philip , Murawski Beatrice , Plotnikoff Ronald , Rayward Anna , Stamatakis Emmanuel , Vandelanotte Corneel , Burrows Tracy
Published: August 2020

Background: This study compared the efficacy of two multi-component m-health interventions with a wait-list control group on body weight (primary outcome), and secondary outcomes of cardiovascular risk factors, lifestyle behaviours, and mental health. Methods: Three-arm randomised controlled trial (Enhanced: physical activity, diet, sleep, Traditional: physical activity, diet, Control) with assessments conducted at baseline, 6 and 12 months. Participants (n = 116) were overweight or obese adults aged 19–65 (M = 44.5 [SD = 10.5]). The 6-month intervention was delivered via a smartphone app providing educational materials, goal-setting, self-monitoring and feedback, and also included one face-to-face dietary consultation, a Fitbit and scales. The trial was prospectively registered and conducted between May 2017 and September 2018. Group differences on primary and secondary outcomes were examined between the Pooled Intervention groups (Pooled Intervention = Enhanced and Traditional) and Control groups, and then between Enhanced and Traditional groups. Results: Nineteen participants (16.4%) formally withdrew from the trial. Compared with the Control group, average body weight of the Pooled Intervention group did not differ at 6 (between-group difference = −0.92, (95% CI −3.33, 1.48)) or 12 months (0.00, (95% CI −2.62, 2.62)). Compared with the Control group, the Pooled Intervention group significantly increased resistance training (OR = 7.83, (95% CI 1.08, 56.63)) and reduced energy intake at 6 months (−1037.03, (−2028.84, −45.22)), and improved insomnia symptoms at 12 months (−2.59, (−4.79, −0.39)). Compared with the Traditional group, the Enhanced group had increased waist circumferences (2.69, (0.20, 5.18)) and sedentary time at 6 months (105.66, (30.83, 180.48)), and improved bed time variability at 12 months (−1.08, (−1.86, −0.29)). No other significant differences were observed between groups. Conclusions: Relative to Controls, the Pooled Intervention groups did not differ on body weight but improved resistance training, and reduced energy intake and insomnia symptom severity. No additional weight loss was apparent when targeting improvements in physical activity, diet and sleep in combination compared with physical activity and diet.
Physical Activity, Step Counts, and Grip Strength in the Chinese Children and Families Cohort Study
Authors: Berrigan David , Liu Ailing , Belcher Britni R. , Chao Ann , Fang Liwen , Matthews Charles E. , Wang Baohua , Wang Linhong , Wang Ning , Wang Yu , Yang Lichen , Linet Martha S. , Potischman Nancy
Published: August 2020

Objectives: This paper describes the development of a physical activity questionnaire (PAQ) designed for Chinese adolescents and their mothers in urban and rural settings, and reports on results of the PAQ, pedometry, and hand grip dynamometry from the Chinese Children and Families Cohort Study pilot investigation (CFCS). Methods: As part of a pilot investigation to evaluate the feasibility to follow-up and obtain detailed nutrition, dietary, physical activity, and ultraviolet radiation (UVR) data from CFCS participants, data were collected in 2013 for 93 adolescent/mother pairs from a rural (n = 41) and an urban site (n = 52) in two provinces. Respondents were asked to wear a pedometer for seven days (Omron HJ-151), use a Takei Digital Grip Strength Dynamometer on (each hand; three trials; two separate days), and complete a 39 item, eight domain PAQ covering the past year. Self-reported physical activity (PA) was linked to metabolic equivalent of task (MET) scores in kcal/kg/hr and used to calculate METs for different domains of PA and intensity categories. Results: Compliance was high (95%) in this measurement protocol administered by health staff during a series of data collection efforts at home and local clinics or health centers. Step counts were highly variable, averaging between 5000 and 10000 per day with somewhat higher step counts in rural adolescent boys. Maximum grip strength (Kgs) was greater in children (Mean = 36.5, SE = 0.8) than mothers (Mean = 28.8, SE = 0.8) and similar in the urban (Mean = 29.6, SE = 0.6) compared to the rural (Mean = 29.6, SE = 0.5) communities overall. Grip strength, step counts, and measures of time spent in different activities or activity intensities were uncorrelated. Conclusion: Device and question-based measurement of PA and strength were readily accepted in these Chinese urban and rural populations. The PAQ on physical activity in the past year produced some plausible population averages, but individual responses suggested recall challenges. If data about specific activities are required, future studies should explore use of standardized survey questions concerning such fewer specific activities or instruments examining shorter time periods such as one, three, or seven day recalls.
Knowing My Village from the Sky: A Collaborative Spatial Learning Framework to Integrate Spatial Knowledge of Stakeholders in Achieving Sustainable Development Goals
Authors: Akbar Aulia , Flacke Johannes , Martinez Javier , Aguilar Rosa , van Maarseveen Martin
Published: August 2020

Geospatial data is urgently needed in decision-making processes to achieve Sustainable Development Goals (SDGs) at global, national, regional and local scales. While the advancement of geo-technologies to obtain or produce geospatial data has become faster and more affordable, many countries in the global south still experience a geospatial data scarcity at the rural level due to complex geographical terrains, weak coordination among institutions and a lack of knowledge and technologies to produce visualised geospatial data like maps. We proposed a collaborative spatial learning framework that integrates the spatial knowledge of stakeholders to obtain geospatial data. By conducting participatory mapping workshops in three villages in the Deli Serdang district in Indonesia, we tested the framework in terms of facilitating communication and collaboration of the village stakeholders while also supporting knowledge co-production and social learning among them. Satellite images were used in digital and non-digital mapping workshops to support village stakeholders to produce proper village maps while fulfilling the SDGs’ emphasis to make geospatial data available through a participatory approach.
Correction: Sunzini, F., et al. Hydrogen Sulfide as a Potential Regulatory Gasotransmitter in Arthritic Diseases. Int. J. Mol. Sci. 2020, 21, 1180; doi:10.3390/ijms21041180
Authors: Sunzini Flavia , De Stefano Susanna , Chimenti Maria Sole , Melino Sonia
Published: August 2020

The authors wish to make the following correction to this paper [...]
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus
Authors: Habib Syahir , Ahmad Siti Aqlima , Wan Johari Wan Lutfi , Abd Shukor Mohd Yunus , Alias Siti Aisyah , Smykla Jerzy , Saruni Nurul Hani , Abdul Razak Nur Syafiqah , Yasid Nur Adeela
Published: August 2020

Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research.
Bioactive Phenolics and Polyphenols: Current Advances and Future Trends
Authors: Jacobo-Velázquez Daniel A. , Cisneros-Zevallos Luis
Published: August 2020

Phenolic compounds are secondary plant metabolites with remarkable health-promoting properties [...]
Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium
Authors: Lee Changrim , Edman Maria C. , Laurie Gordon W. , Hamm-Alvarez Sarah F. , MacKay J. Andrew
Published: August 2020

Lacripep is a therapeutic peptide derived from the human tear protein, Lacritin. Lacripep interacts with syndecan-1 and induces mitogenesis upon the removal of heparan sulfates (HS) that are attached at the extracellular domain of syndecan-1. The presence of HS is a prerequisite for the syndecan-1 clustering that stimulates exosome biogenesis and release. Therefore, syndecan-1-mediated mitogenesis versus HS-mediated exosome biogenesis are assumed to be mutually exclusive. This study introduces a biosynthesized fusion between Lacripep and an elastin-like polypeptide named LP-A96, and evaluates its activity on cell motility enhancement versus exosome biogenesis. LP-A96 activates both downstream pathways in a dose-dependent manner. HCE-T cells at high confluence treated with 1 μM LP-A96 enhanced cell motility equipotent to Lacripep. However, cells at low density treated with 1 μM LP-A96 generated a 210-fold higher number of exosomes compared to those treated at low density with Lacripep. As monovalent Lacripep is capable of enhancing cell motility but not exosome biogenesis, activation of exosome biogenesis by LP-A96 not only suggests its utility as a novel molecular tool to study the Lacritin biology in the corneal epithelium but also implies activity as a potential therapeutic peptide that can further improve ocular surface health through the induction of exosomes.
Fluorescence Lifetime and Intensity of Thioflavin T as Reporters of Different Fibrillation Stages: Insights Obtained from Fluorescence Up-Conversion and Particle Size Distribution Measurements
Authors: Rovnyagina Nataliya R. , Budylin Gleb S. , Vainer Yuri G. , Tikhonova Tatiana N. , Vasin Sergey L. , Yakovlev Alexander A. , Kompanets Victor O. , Chekalin Sergey V. , Priezzhev Alexander V. , Shirshin Evgeny A.
Published: August 2020

Thioflavin T (ThT) assay is extensively used for studying fibrillation kinetics in vitro. However, the differences in the time course of ThT fluorescence intensity and lifetime and other physical parameters of the system, such as particle size distribution, raise questions about the correct interpretation of the aggregation kinetics. In this work, we focused on the investigation of the mechanisms, which underlay the difference in sensitivity of ThT fluorescence intensity and lifetime to the formation of protein aggregates during fibrillation by the example of insulin and during binding to globular proteins. The assessment of aggregate sizes and heterogeneity was performed using dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). Using the sub-nanosecond resolution measurements, it was shown that the ThT lifetime is sensitive to the appearance of as much as a few percent of ThT bound to the high-affinity sites that occur simultaneously with an abrupt increase of the average particle size, particles concentration, and size heterogeneity. The discrepancy between ThT fluorescence intensity and a lifetime can be explained as the consequence of a ThT molecule fraction with ultrafast decay and weak fluorescence. These ThT molecules can only be detected using time-resolved fluorescence measurements in the sub-picosecond time domain. The presence of a bound ThT subpopulation with similar photophysical properties was also demonstrated for globular proteins that were attributed to non-specifically bound ThT molecules with a non-rigid microenvironment.
CRISPR/Cas9 Directed Mutagenesis of OsGA20ox2 in High Yielding Basmati Rice (Oryza sativa L.) Line and Comparative Proteome Profiling of Unveiled Changes Triggered by Mutations
Authors: Nawaz Gul , Usman Babar , Zhao Neng , Han Yue , Li Zhihua , Wang Xin , Liu Yaoguang , Li Rongbai
Published: August 2020

In rice, semi-dwarfism is among the most required characteristics, as it facilitates better yields and offers lodging resistance. Here, semi-dwarf rice lines lacking any residual transgene-DNA and off-target effects were generated through CRISPR/Cas9-guided mutagenesis of the OsGA20ox2 gene in a high yielding Basmati rice line, and the isobaric tags for relative and absolute quantification (iTRAQ) strategy was utilized to elucidate the proteomic changes in mutants. The results indicated the reduced gibberellins (GA1 and GA4) levels, plant height (28.72%), and flag leaf length, while all the other traits remained unchanged. The OsGA20ox2 expression was highly suppressed, and the mutants exhibited decreased cell length, width, and restored their plant height by exogenous GA3 treatment. Comparative proteomics of the wild-type and homozygous mutant line (GXU43_9) showed an altered level of 588 proteins, 273 upregulated and 315 downregulated, respectively. The identified differentially expressed proteins (DEPs) were mainly enriched in the carbon metabolism and fixation, glycolysis/gluconeogenesis, photosynthesis, and oxidative phosphorylation pathways. The proteins (Q6AWY7, Q6AWY2, Q9FRG8, Q6EPP9, Q6AWX8) associated with growth-regulating factors (GRF2, GRF7, GRF9, GRF10, and GRF11) and GA (Q8RZ73, Q9AS97, Q69VG1, Q8LNJ6, Q0JH50, and Q5MQ85) were downregulated, while the abscisic stress-ripening protein 5 (ASR5) and abscisic acid receptor (PYL5) were upregulated in mutant lines. We integrated CRISPR/Cas9 with proteomic screening as the most reliable strategy for rapid assessment of the CRISPR experiments outcomes.
Multi-Fault Diagnosis Approach Based on Updated Interacting Multiple Model for Aviation Hydraulic Actuator
Authors: Sun Xiaozhe , Wang Xingjian , Lin Siru
Published: August 2020

The aviation hydraulic actuator (HA) is a key component of the flight control system in an aircraft. It is necessary to consider the occurrence of multiple faults under harsh conditions during a flight. This study designs a multi-fault diagnosis method based on the updated interacting multiple model (UIMM). The correspondence between the failure modes and the key physical parameters of HA is found by analyzing the fault mode and mechanism. The key physical parameters of HA can be estimated by employing a series of extended Kalman filters (EKF) related to the different modes of HA. The models in UIMM are updated once the fault is determined. UIMM can reduce the number of fault models and avoid combinatorial explosion in the case of multiple faults. Simulation results indicate that the multi-fault diagnosis method based on UIMM is effective for multi-fault diagnosis of electro-hydraulic servo actuation system.
Assessment of the Economic and Social Impact of Shared Parking in Residential Areas
Authors: Ye Xiaofei , Sui Xinliu , Xie Jin , Wang Tao , Yan Xingchen , Chen Jun
Published: August 2020

Shared parking schemes are not commonly implemented in residential areas due to the uncertainty and conflicts associated with the benefits of such schemes for stakeholders, namely, parking suppliers, parking managers, and the public. To evaluate the economic and social impacts of shared parking in residential areas on its stakeholders, the risk and benefit factors were determined through influential analysis and a questionnaire. A risk–benefit model was established to quantify the risks and benefits for stakeholders. The social return on investment and sensitivity analysis were applied to estimate the economic feasibility of shared parking in residential areas. The methodology combined the use of qualitative, quantitative, and financial information gathered and analyzed to estimate the “value” of shared parking, including its risks, benefits, management pressure, and social benefit. The model was calibrated using the survey data collected from the city of Ningbo in China. The results showed that: (1) The net present value was negative, indicating that the benefits of shared parking were lower than the risks, and thus this scheme would not be economically feasible in residential areas. (2) The cost of purchasing new equipment and rebuilding parking lots had the greatest impact on the benefits of shared parking in residential areas, with a sensitivity coefficient of 4.396, followed by the income from shared parking charges (3.885), and the salary of parking managers (3.619). (3) If the income from parking charges and the salary of parking managers were more than 69,408.5 and 31,091.1 yuan per month, respectively, and the cost of improving parking infrastructure was less than 14,003.2 yuan per month, residential areas could obtain additional benefits due to the acceptance of a shared parking scheme. This study provides theoretical support for the reasonable determination of the costs, risks, and benefits associated with participating in a shared parking scheme in a residential area.
Stannous Fluoride Preventive Effect on Enamel Erosion: An In Vitro Study
Authors: Lucchese Alessandra , Bertacci Angelica , Lo Giudice Antonino , Polizzi Elisabetta , Gherlone Enrico , Manuelli Maurizio , Chersoni Stefano , Moro Daniele , Valdrè Giovanni
Published: August 2020

The aim of this in vitro study was to evaluate the effects of a single dose application of two daily toothpastes on enamel exposed to acid attack. The research was conducted on human molars enamel fragments (n = 72). The two different toothpastes active ingredients were sodium fluoride (NaF) and stannous fluoride (SnF2). They were compared in protecting the surface of the enamel exposed to three acids: citric acid, lactic acid and hydrochloric acid. A spectrophotometer was used to measure the calcium ions and phosphate released in the solutions by the enamel specimens. Afterward, ionic concentrations were analyzed through the t-Student test, in order to estimate the significance level (p < 0.05) of the solubility differences obtained between the treatment and control groups. Finally, sample surfaces were analyzed with scanning electron microscopy and X-ray energy dispersive spectroscopy (SEM/EDX). The two analyzed toothpastes did not reveal any statistically significant variation in the release of calcium and phosphate (p > 0.05). Nevertheless, acid-resistant deposits were detected in samples treated with stannous fluoride and exposed to lactic acid, though the presence of tin ion deposits on samples treated with stannous fluoride was not shown. A single dose of a fluoride-based toothpaste before different acids attack, in simulated oral cavity conditions, did not show a significant preventive effect.
The Effect of Environmental Stressors on Tinnitus: A Prospective Longitudinal Study on the Impact of the COVID-19 Pandemic
Authors: Schlee Winfried , Hølleland Sondre , Bulla Jan , Simoes Jorge , Neff Patrick , Schoisswohl Stefan , Woelflick Stella , Schecklmann Martin , Schiller Axel , Staudinger Susanne , Probst Thomas , Langguth Berthold
Published: August 2020

Tinnitus, the perception of sound in the absence of a corresponding sound, and the distress caused by it, is rarely a static phenomenon. It rather fluctuates over time depending on endogenous and exogenous factors. The COVID-19 pandemic is a potential environmental stressor that might influence the individually perceived tinnitus distress. Since not all people are affected by the pandemic in the same way, the situation allows one to identify environmental factors and personality traits that impact tinnitus distress differently. In our study, 122 tinnitus patients were included at two time points: in the year 2018 and during the German lockdown in April 2020. We assessed tinnitus-related distress, depressive symptoms, personality characteristics and the individual perception of the pandemic situation. On average, there was only a small increase of tinnitus distress with heterogeneous changes during the lockdown. People perceiving the situation as generally stressful with increased grief, frustration, stress and nervousness reported the worsening of tinnitus distress. People with high values in neuroticism also reported the worsening of tinnitus distress, while the personality traits extraversion, conscientiousness and openness seemed to be a protection factor. The study identifies factors that influence tinnitus distress change during a pandemic and spots those patients that need specific help in the pandemic situation.
Understanding the Relationship between Glutathione, TGF-β, and Vitamin D in Combating Mycobacterium tuberculosis Infections
Authors: Singh Mohkam , Vaughn Charles , Sasaninia Kayvan , Yeh Christopher , Mehta Devanshi , Khieran Ibrahim , Venketaraman Vishwanath
Published: August 2020

Tuberculosis (TB) remains a pervasive global health threat. A significant proportion of the world’s population that is affected by latent tuberculosis infection (LTBI) is at risk for reactivation and subsequent transmission to close contacts. Despite sustained efforts in eradication, the rise of multidrug-resistant strains of Mycobacteriumtuberculosis (M. tb) has rendered traditional antibiotic therapy less effective at mitigating the morbidity and mortality of the disease. Management of TB is further complicated by medications with various off-target effects and poor compliance. Immunocompromised patients are the most at-risk in reactivation of a LTBI, due to impairment in effector immune responses. Our laboratory has previously reported that individuals suffering from Type 2 Diabetes Mellitus (T2DM) and HIV exhibited compromised levels of the antioxidant glutathione (GSH). Restoring the levels of GSH resulted in improved control of M. tb infection. The goal of this review is to provide insights on the diverse roles of TGF- β and vitamin D in altering the levels of GSH, granuloma formation, and clearance of M. tb infection. We propose that these pathways represent a potential avenue for future investigation and development of new TB treatment modalities.
Perinatal Outcome and Long-Term Infectious Morbidity of Offspring Born to Women with Known Tuberculosis
Authors: Sade Shanny , Wainstock Tamar , Sheiner Eyal , Pariente Gali
Published: August 2020

Objective: To evaluate the perinatal outcome of women with tuberculosis and to assess a possible association between maternal tuberculosis and long-term infectious morbidity of the offspring. Study design: Perinatal outcome and long-term infectious morbidity of offspring of mothers with and without tuberculosis were assessed. The study groups were followed until 18 years of age tracking infectious-related morbidity and infectious-related hospitalizations and then compared. For perinatal outcome, generalized estimation equation models were used. A Kaplan-Meier survival curve was used to compare cumulative incidence of long-term infectious morbidity. A Cox proportional hazards model was conducted to control for confounders. Results: During the study period, 243,682 deliveries were included, of which 46 (0.018%) occurred in women with tuberculosis. Maternal tuberculosis was found to be independently associated with placental abruption, cesarean deliveries, and very low birth weight. However, offspring born to mothers with tuberculosis did not demonstrate higher rates of infectious-related morbidity. Maternal tuberculosis was not noted as an independent risk factor for long-term infectious morbidity of the offspring. Conclusion: In our study, maternal tuberculosis was found to be independently associated with adverse perinatal outcomes. However, higher risk for long-term infectious morbidity of the offspring was not demonstrated. Careful surveillance of these women is required.
Increased Elastin Degradation in Pseudoxanthoma Elasticum Is Associated with Peripheral Arterial Disease Independent of Calcification
Authors: Bartstra Jonas W. , Spiering Wilko , van den Ouweland Jody M. W. , Mali Willem P. T. M. , Janssen Rob , de Jong Pim A.
Published: August 2020

Pseudoxanthoma elasticum (PXE) results in extensive fragmentation and calcification of elastin fibers in the peripheral arteries, which results in peripheral arterial disease (PAD). Current research focuses on the role of calcifications in the pathogenesis of PXE. Elastin degradation and calcification are shown to interact and may amplify each other. This study aims to compare plasma desmosines, a measure of elastin degradation, between PXE patients and controls and to investigate the association between desmosines and (1) arterial calcification, (2) PAD, and (3) PAD independent of arterial calcification in PXE. Plasma desmosines were quantified with liquid chromatography-tandem mass spectrometry in 93 PXE patients and 72 controls. In PXE patients, arterial calcification mass was quantified on CT scans. The ankle brachial index (ABI) after treadmill test was used to analyze PAD, defined as ABI < 0.9, and the Fontaine classification was used to distinguish symptomatic and asymptomatic PAD. Regression models were built to test the association between desmosines and arterial calcification and arterial functioning in PXE. PXE patients had higher desmosines than controls (350 (290–410) ng/L vs. 320 (280–360) ng/L, p = 0.02). After adjustment for age, sex, body mass index, smoking, type 2 diabetes mellitus, and pulmonary abnormalities, desmosines were associated with worse ABI (β (95%CI): −68 (−132; −3) ng/L), more PAD (β (95%CI): 40 (7; 73) ng/L), and higher Fontaine classification (β (95%CI): 30 (6; 53) ng/L), but not with arterial calcification mass. Lower ABI was associated with higher desmosines, independent from arterial calcification mass (β (95%CI): −0.71(−1.39; −0.01)). Elastin degradation is accelerated in PXE patients compared to controls. The association between desmosines and ABI emphasizes the role of elastin degradation in PAD in PXE. Our results suggest that both elastin degradation and arterial calcification independently contribute to PAD in PXE.
Implication of Aging Related Chronic Neuroinflammation on COVID-19 Pandemic
Authors: Bossù Paola , Toppi Elisa , Sterbini Valentina , Spalletta Gianfranco
Published: August 2020

SARS-CoV-2, the virus responsible for the COVID-19 pandemic, leads to a respiratory syndrome and other manifestations. Most affected people show no or mild symptoms, but the risk of severe disease and death increases in older people. Here, we report a narrative review on selected studies targeting aging-related chronic neuroinflammation in the COVID-19 pandemic. A hyperactivation of the innate immune system with elevated levels of pro-inflammatory cytokines occurs during severe COVID-19, pointing to an important role of the innate immune dysregulation in the disease outcome. Aging is characterized by a general condition of low-grade inflammation, also connected to chronic inflammation of the brain (neuroinflammation), which is involved in frailty syndrome and contributes to several age-associated diseases, including neurodegenerative and neuropsychiatric disorders. Since neuroinflammation can be induced or worsened by the virus infection itself, as well as by stressful conditions like those linked to the recent pandemic, the role of neuroinflammatory mechanisms could be central in a vicious circle leading to an increase in the mortality risk in aged COVID-19 patients. Furthermore, triggered neuroinflammatory pathways and consequent neurodegenerative and neuropsychiatric conditions might be potential long-term complications of COVID-19. In order to provide insights to help clinicians in identifying patients who progress to a more severe case of the disease, this review underlines the potential implications of aging-related neuroinflammation in COVID-19 pandemic.
Integrating Ecosystem Services and Disservices in Valuing Smallholder Livestock and Poultry Production in Three Villages in South Africa
Authors: Herd-Hoare Shannon C. , Shackleton Charlie M.
Published: August 2020

This study presents an integrated examination of both the ecosystem services (ES) and ecosystem disservices (EDS) associated with smallholder animal husbandry in rural livelihoods in three villages in southeast South Africa. It recognises the contribution of ES supporting and resulting from smallholder livestock and poultry production, but also details the limiting factors or EDS, such as tick-borne disease, birds of prey or unpalatable rangeland, produced by the same system. Using a mixed-methods approach, including focus group discussions with various Participatory Learning and Action (PLA) activities, key informant interviews, household surveys and land-use change mapping on GIS, we consider the relative value and benefits from ES after the effects of EDS, as well as the management and strategies that households adopt to minimise EDS. The effects of ES and EDS were expressed in economic terms to provide a common framework to assess the magnitude of their contribution or effect. Although animal husbandry made measurable contributions to households, with an average gross value of between R2605–R9753 across villages, EDS undermined the meaningful production of livestock and poultry goods and services. The average EDS-induced economic loss in households was between R8289–R22,426 per annum. Despite active management, often at substantial cost to the household, EDS undermined the health of livestock which resulted in a loss of the animal or potential goods and services produced. We emphasise the need to recognise both the positive and negative contributions of ecosystems to identify the complex feedbacks between ES and EDS which interact to determine local ways of doing things.
Ways Forward for Advancing Ecosystem Services in Municipal Planning—Experiences from Stockholm County
Authors: Khoshkar Sara , Hammer Monica , Borgström Sara , Balfors Berit
Published: August 2020

This case study from Stockholm County, Sweden, explores practitioners’ experiences of barriers and bridges in municipal planning practices to support actions for ecosystem services. This qualitative study is based on information gathered from a focus group, workshops, and semi-structured interviews, which aided in identifying key factors for integrating ecosystem services in municipal planning. We identified 10 key factors divided into three themes: (i) regulatory framework and political support, (ii) local organizational capacity, and (iii) local adaptation of tools and practices. In particular, the practitioners pointed to the need for the development of legal support and regulations for ecosystem services on the national and EU policy levels. Furthermore, the need for local capacity building and understanding of ecosystem services as well as increased regional support to enhance local knowledge exchange and learning was emphasized. Also, in a decentralized local governance system such as in Sweden, to fully implement ecosystem services in urban planning for sustainable development, locally adapted practical tools and monitoring procedures were considered important.
The Maintenance of Mitochondrial DNA Integrity and Dynamics by Mitochondrial Membranes
Authors: Chapman James , Ng Yi Shiau , Nicholls Thomas J.
Published: August 2020

Mitochondria are complex organelles that harbour their own genome. Mitochondrial DNA (mtDNA) exists in the form of a circular double-stranded DNA molecule that must be replicated, segregated and distributed around the mitochondrial network. Human cells typically possess between a few hundred and several thousand copies of the mitochondrial genome, located within the mitochondrial matrix in close association with the cristae ultrastructure. The organisation of mtDNA around the mitochondrial network requires mitochondria to be dynamic and undergo both fission and fusion events in coordination with the modulation of cristae architecture. The dysregulation of these processes has profound effects upon mtDNA replication, manifesting as a loss of mtDNA integrity and copy number, and upon the subsequent distribution of mtDNA around the mitochondrial network. Mutations within genes involved in mitochondrial dynamics or cristae modulation cause a wide range of neurological disorders frequently associated with defects in mtDNA maintenance. This review aims to provide an understanding of the biological mechanisms that link mitochondrial dynamics and mtDNA integrity, as well as examine the interplay that occurs between mtDNA, mitochondrial dynamics and cristae structure.
Milling of an Al/CFRP Sandwich Construction with Non-Coated and TiAlN-Coated Tools
Authors: Doluk Elżbieta , Rudawska Anna , Kuczmaszewski Józef , Pieśko Paweł
Published: August 2020

In this study, the effect of cutting parameters on the quality of an Al/CFRP sandwich structure (aluminium alloy–carbon fibre reinforced polymer) after milling with uncoated and TiAlN-coated tools was examined. The results of the cutting force were also investigated. The research was conducted in a VMC 800 HS vertical machining centre with a variable cutting speed and feed. The milling process was carried out using a non-coated, two-blade carbide milling cutter with a 35° helix angle and an analogous tool with a TiAlN coating. The surface quality was characterised in terms of the height deviation, which is one of the shape deviations after machining hybrid materials. The research showed that the maximum (77.60 µm) and minimum (1.78 µm) values of the height deviations were obtained using the tool with a TiAlN coating. It was found that the tested factors had significant effects on the height deviation, where the feed had the greatest influence and the cutting speed had the lowest influence on the surface quality. The tested factors were not statistically significant in terms of the cutting force.
Effect of Incorporated ZnO Nanoparticles on the Corrosion Performance of SiO2 Nanoparticle-Based Mechanically Robust Epoxy Coatings
Authors: Abdus Samad Ubair , Alam Mohammad Asif , Anis Arfat , Sherif El-Sayed M. , Al-Mayman Sulaiman I. , Al-Zahrani Saeed M.
Published: August 2020

This paper presents the studies of the development of a high-performance epoxy coating for steel substrates. To this end, it investigated the synergistic effect of incorporating zinc oxide (ZnO) nanoparticles into nanosilica containing epoxy formulations. The mechanical properties of the epoxy coating formulations were improved by modifying the surfaces of the silica nanoparticles (5 wt.%) with 3-glycidoxypropyl trimethoxysilane, which ensured their dispersal through the material. Next, the ZnO nanoparticles (1, 2, or 3 wt.%) were incorporated to improve the corrosion performance of the formulations. The anticorrosive properties of the coatings were examined by electrochemical impedance spectroscopy (EIS) of coated mild steel specimens immersed in 3.5% NaCl solution over different time intervals (1 h to 30 days). Incorporation of the ZnO nanoparticles and the nanosilica into the coating formulation improved the corrosion resistance of the epoxy coating even after long-term exposure to saline test solutions. Finally, to evaluate how the nanoparticles affected the chemical and morphological properties of the prepared coatings, the coatings were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD).
Influence of Laser Powder Bed Fusion Process Parameters on Voids, Cracks, and Microhardness of Nickel-Based Superalloy Alloy 247LC
Authors: Adegoke Olutayo , Andersson Joel , Brodin Håkan , Pederson Robert
Published: August 2020

The manufacturing of parts from nickel-based superalloy Alloy 247LC by laser powder bed fusion (L-PBF) is challenging, primarily owing to the alloy’s susceptibility to cracks. Apart from the cracks, voids created during the L-PBF process should also be minimized to produce dense parts. In this study, samples of Alloy 247LC were manufactured by L-PBF, several of which could be produced with voids and crack density close to zero. A statistical design of experiments was used to evaluate the influence of the process parameters, namely laser power, scanning speed, and hatch distance (inherent to the volumetric energy density) on void formation, crack density, and microhardness of the samples. The window of process parameters, in which minimum voids and/or cracks were present, was predicted. It was shown that the void content increased steeply at a volumetric energy density threshold below 81 J/mm3. The crack density, on the other hand, increased steeply at a volumetric energy density threshold above 163 J/mm3. The microhardness displayed a relatively low value in three samples which displayed the lowest volumetric energy density and highest void content. It was also observed that two samples, which displayed the highest volumetric energy density and crack density, demonstrated a relatively high microhardness; which could be a vital evidence in future investigations to determine the fundamental mechanism of cracking. The laser power was concluded to be the strongest and statistically most significant process parameter that influenced void formation and microhardness. The interaction of laser power and hatch distance was the strongest and most significant factor that influenced the crack density.
Poly(l-Lactic Acid)/Pine Wood Bio-Based Composites
Authors: Dobrzyńska-Mizera Monika , Knitter Monika , Woźniak-Braszak Aneta , Baranowski Mikołaj , Sterzyński Tomasz , Di Lorenzo Maria Laura
Published: August 2020

Bio-based composites made of poly(l-lactic acid) (PLLA) and pine wood were prepared by melt extrusion. The composites were compatibilized by impregnation of wood with γ-aminopropyltriethoxysilane (APE). Comparison with non-compatibilized formulation revealed that APE is an efficient compatibilizer for PLLA/wood composites. Pine wood particles dispersed within PLLA act as nucleating agents able to start the growth of PLLA crystals, resulting in a faster crystallization rate and increased crystal fraction. Moreover, the composites have a slightly lower thermal stability compared to PLLA, proportional to filler content, due to the lower thermal stability of wood. Molecular dynamics was investigated using the solid-state 1H NMR technique, which revealed restrictions in the mobility of polymer chains upon the addition of wood, as well as enhanced interfacial adhesion between the filler and matrix in the composites compatibilized with APE. The enhanced interfacial adhesion in silane-treated composites was also proved by scanning electron microscopy and resulted in slightly improved deformability and impact resistance of the composites.