bluuebunny/crossref_metadata_2025 huggingface dataset:
Intracellular Localization during Blood–Brain Barrier Crossing Influences Extracellular Release and Uptake of Fluorescent Nanoprobes
Authors: Muscetti Ornella , Blal Naym , Mollo Valentina , Netti Paolo Antonio , Guarnieri Daniela
Published: July 2023

To improve the efficacy of nanoparticles (NPs) and boost their theragnostic potential for brain diseases, it is key to understand the mechanisms controlling blood–brain barrier (BBB) crossing. Here, the capability of 100 nm carboxylated polystyrene NPs, used as a nanoprobe model, to cross the human brain endothelial hCMEC/D3 cell layer, as well as to be consequently internalized by human brain tumor U87 cells, is investigated as a function of NPs’ different intracellular localization. We compared NPs confined in the endo-lysosomal compartment, delivered to the cells through endocytosis, with free NPs in the cytoplasm, delivered by the gene gun method. The results indicate that the intracellular behavior of NPs changed as a function of their entrance mechanism. Moreover, by bypassing endo-lysosomal accumulation, free NPs were released from cells more efficiently than endocytosed NPs. Most importantly, once excreted by the endothelial cells, free NPs were released in the cell culture medium as aggregates smaller than endocytosed NPs and, consequently, they entered the human glioblastoma U87 cells more efficiently. These findings prove that intracellular localization influences NPs’ long-term fate, improving their cellular release and consequent cellular uptake once in the brain parenchyma. This study represents a step forward in designing nanomaterials that are able to reach the brain effectively.
Women in Selected Communities of Punjab, India Have a High Prevalence of Iron, Zinc, Vitamin B12, and Folate Deficiencies: Implications for a Multiply-Fortified Salt Intervention
Authors: Goh Yvonne E. , Manger Mari S. , Duggal Mona , Das Reena , Saklani Shipra , Agarwal Surbhi , Budhija Deepmala , Jamwal Manu , Singh Bidhi L. , Dahiya Neha , Luo Hanqi , Long Julie M. , Westcott Jamie , Krebs Nancy F. , Gibson Rosalind S. , Brown Kenneth H. , McDonald Christine M.
Published: July 2023

Dietary intake and biomarkers of micronutrient status of 100 non-pregnant women of reproductive age (NPWRA) were assessed to determine optimal levels of iron, zinc, vitamin B12, and folic acid to include in multiply-fortified salt (MFS) that will be evaluated in an upcoming trial. Weighed food records were obtained from participants to measure intake of micronutrients and discretionary salt, and to assess adequacy using Indian Nutrient Reference Values (NRVs). Statistical modeling was used to determine optimal fortification levels to reduce inadequate micronutrient intake while limiting intake above the upper limit. Fasting blood samples were obtained to assess iron, zinc, vitamin B12, and folate status. In usual diets, inadequate intake of iron (46%), zinc (95%), vitamin B12 (83%), and folate (36%) was high. Mean intake of discretionary salt was 4.7 g/day. Prevalence estimates of anemia (37%), iron deficiency (67%), zinc deficiency (34%), vitamin B12 insufficiency (37%), and folate insufficiency (70%) were also high. Simulating the addition of optimized MFS to usual diets resulted in percentage point (pp) reductions in inadequate intake by 29 pp for iron, 76 pp for zinc, 81 pp for vitamin B12, and 36 pp for folate. MFS holds potential to reduce the burden of micronutrient deficiencies in this setting.
Prevalence of Vitamin B12 and Folate Deficiencies in Indian Children and Adolescents
Authors: Shalini Tattari , Pullakhandam Raghu , Ghosh Santu , Kulkarni Bharati , Rajkumar Hemalatha , Sachdev Harshpal S. , Kurpad Anura V. , Reddy Geereddy Bhanuprakash
Published: July 2023

Deficiencies of vitamin B12 (B12) and folate (FA) are of particular interest due to their pleiotropic role in 1-carbon metabolism. In addition to adverse birth outcomes, deficiencies of B12 and FA, or an imbalance in FA/B12 status, are linked to metabolic disorders. Indian diets that are predominantly plant food-based could be deficient in these vitamins, but there are no national estimates of the prevalence of B12 and FA deficiency in Indian children and adolescents, nor their associations with age, sex and growth indicators. The recent Comprehensive National Nutrition Survey (CNNS-2016-18) provided estimates of the prevalence of B12 and FA deficiency at the national and state levels among preschool (1–4 years: 9976 and 11,004 children, respectively), school-age children (5–9 years: 12,156 and 14,125) and adolescents (10–19 years: 11,748 and 13,621). Serum B12 and erythrocyte FA were measured by the direct chemiluminescence method and their deficiency was defined using WHO cut-offs. The prevalence of B12 and FA deficiency was high among adolescents (31.0%, CI: 28.7–33.5 and 35.6%, CI: 33.1–8.2) compared to school-age (17.3%, CI: 15.4–19.3 and 27.6%, CI: 25.5–29.9) and preschool children (13.8%, CI: 11.7–16.2 and 22.8%, CI: 20.5–25.2, respectively). The prevalence of both B12 and FA deficiency was significantly higher by 8% and 5%, respectively, in adolescent boys compared to girls. There was no association between anthropometric undernutrition and B12 and FA deficiency. There was wide regional variation in the prevalence of B12 and FA deficiency, but no rural–urban differences were observed across all age groups. The national prevalence of B12 deficiency among preschool or school-age children was <20% (the cut-off that indicates a public health problem). However, FA deficiency in these age groups and both FA and B12 deficiencies in adolescents were >20%, warranting further investigation.
Characterization of an Escherichia coli Isolate Coharboring the Virulence Gene astA and Tigecycline Resistance Gene tet(X4) from a Dead Piglet
Authors: Wang Jianmei , Huang Yuting , Guan Chunjiu , Li Jie , Yang Hua , Zhao Guoping , Liu Canying , Ma Jiangang , Tang Biao
Published: July 2023

tet(X4) is the critical resistance gene for tigecycline degradation that has been continually reported in recent years. In particular, pathogenic bacteria carrying tet(X4) are a severe threat to human health. However, information describing Escherichia coli coharboring tet(X4) with virulence genes is limited. Here, we isolated an E. coli strain coharboring tet(X4) and the heat-stable toxin gene astA from a dead piglet. The strain named 812A1-131 belongs to ST10. The genome was sequenced using the Nanopore and Illumina platforms. The virulence genes astA and tet(X4) are located on the chromosome and in the IncHI1-type plasmid p812A1-tetX4-193K, respectively. The plasmid could be conjugatively transferred to recipient E. coli J53 with high frequency. In vivo experiments showed that strain 812A1-131 is pathogenic to Galleria mellonella and could colonize the intestines of mice. In summary, pathogenic E. coli could receive a plasmid harboring the tet(X4) gene, which can increase the difficulty of treatment. The prevalence and transmission mechanisms of pathogenic bacteria coharboring the tet(X4) gene need more attention.
Oxygen Availability on the Application of Antimicrobial Photodynamic Therapy against Multi-Species Biofilms
Authors: Nie Min , Yang Jingmei , Rastelli Alessandra , Shen Yuqin , Deng Dongmei
Published: July 2023

Methylene blue (MB)- or Curcumin (Cur)-based photodynamic therapy (PDT) has been used as an adjunctive treatment for periodontitis. Its actual clinical efficacy is still in question because the lack of oxygen in a deep periodontal pocket might reduce the PDT efficacy. We aim to investigate the effect of oxygen on PDT efficacy and to examine if the addition of hydrogen peroxide (HP) could improve PDT performance anaerobically. To this end, we cultured 48 h saliva-derived multi-species biofilms and treated the biofilms with 25 µM MB or 40 µM Cur, HP (0.001%, 0.01% and 0.1%), light (L-450 nm or L-660 nm), or combinations thereof under ambient air or strictly anaerobic conditions. MB- and Cur-PDTs significantly reduced biofilm viability in air but not under anaerobic conditions. HP at 0.1% significantly enhanced the killing efficacies of both MB- and Cur-PDTs anaerobically. The killing efficacy of Cur-PDT combined with 0.1% HP was higher anaerobically than in air. However, this was not the case for MB-PDT combined with 0.1% HP. In conclusion, this study demonstrated that the biofilm killing efficacies of MB- and Cur-PDTs diminished when there was no oxygen. HP at 0.1% can enhance the efficacy of PDT performed anaerobically, but the level of enhancement is photosensitizer-dependent.
CYP2D6 Genotype and Pharmacovigilance Impact on Autism Spectrum Disorder: A Naturalistic Study with Extreme Phenotype Analysis
Authors: Ballester Pura , Espadas Cristina , Almenara Susana , Barrachina Jordi , Muriel Javier , Ramos Enrique , Toral Natalia , Belda César , Peiró Ana M.
Published: July 2023

The long-term use of psychopharmacology medications in autism spectrum disorder (ASD) hitherto remains controversial due to a lack of evidence about safety and tolerability. In this regard, genotyping the metabolizing enzyme cytochrome P450 (CYP) 2D6, especially its extreme phenotypes, could help to prevent drug-related adverse reactions or adverse events (AEs). There are several medications warranting CYP2D6 screening that are consumed by people with ASD, such as risperidone and aripiprazole to name a few. A naturalistic observational study was carried out in participants with ASD to analyze the influence of the CYP2D6 phenotype in drug tolerability using a local pharmacovigilance system created for this study. In this case, AEs were identified from participants’ electronic health records (EHRs) and paper registries. Other variables were collected: socio-demographic information, comorbidities, and psychopharmacology prescriptions (polypharmacy defined as ≥4 simultaneous prescriptions) and doses. The genetic analysis included allelic discrimination (CYP2D6*1, *2, *3, *4, *5, *6, *10, *17, and *41) and copy number variations. All of these were used to determine theoretical phenotypes of the metabolic profiles: poor (PM); intermediate (IM); normal (NM); and ultra-rapid (UM). Sex differences were analyzed. A total of 71 participants (30 ± 10 years old, 82% male, 45% CYP2D6 NM phenotype (32 participants)) with a median of 3 (IQR 2–4) comorbidities per person, mainly urinary incontinence (32%) and constipation (22%), were included. CYP2D6 UM showed the highest rate of polypharmacy, whilst, IM participants had the highest rates of neurological and psychiatric AEs, even worse if a CYP2D6 inhibitor drug was prescribed simultaneously. CYP2D6 pharmacogenomics and the monitoring of new antipsychotic prescriptions may make a difference in medication safety in adults with ASD. Particularly in those with psychopharmacology polymedication, it can help with AE avoidance and understanding.
Dose Optimization and Targeting Strategies of Anti-Infective Agents
Authors: Šíma Martin , Slanař Ondřej
Published: July 2023

Most drugs are currently developed, approved and marketed based on their effect on the majority of the population [...]
E. coli Cell Lysis Induced by Lys394 Enzyme Assisted by Magnetic Nanoparticles Exposed to Non-Heating Low-Frequency Magnetic Field
Authors: Usvaliev Azizbek D. , Belogurova Natalia G. , Pokholok Konstantin V. , Finko Alexander V. , Prusov Andrey N. , Golovin Dmitry Yu. , Miroshnikov Konstantin A. , Golovin Yuri I. , Klyachko Natalia L.
Published: July 2023

The spreading of microbial pathogens with more and more resistance to traditional low-molecular antibiotic agents demands new approaches to antibacterial therapy. The employment of bacteriophage enzymes capable of breaking bacterial cell walls has attracted much interest within this context. The specific features of the morphology of Gram-negative bacteria prevent the effective direct usage of lytic enzymes and require assistance from additional helpers to facilitate cell lysis. The current work is devoted to the study of boosting the lysis of Escherichia coli (E. coli) JM 109 and MH 1 strains induced by Lys394 bacteriophage endolysin by means of rod-like (56 × 13 nm) magnetic nanoparticles (MNPs) activated by a non-heating low-frequency magnetic field (LF MF) with a frequency of 50 Hz and a flux density of 68.5 mT in a pulse–pause mode (1 s on and 0.3 s off). According to theoretical assumptions, the mechanism of MNP assistance is presumably based upon the disordering of the outer membrane that facilitates enzyme permeation into peptidoglycans to its substrate. It is found that the effect of the LF MF reaches an almost a twofold acceleration of the enzyme reaction, resulting in almost 80 and 70%, respectively, of lysed E. coli JM 109 and MH 1 cells in 21 min. An increase in the membrane permeability was proven by two independent experiments employing β-lactamase periplasmic enzyme leakage and Nile Red (NR) hydrophobic dye fluorescence. It is shown that the outer membrane disordering of E. coli caused by exposure to LF MF nanoparticle movement leads to almost complete (more than 80%) β-lactamase release out of the cells’ periplasm to the buffer suspension. Experiments with NR (displaying fluorescence in a non-polar medium only) reveal a drastic reduction in NR fluorescence intensity, reaching a change of an order of magnitude when exposed to LF MF. The data obtained provide evidence of changes in the bacterial cell wall structure. The result shown open up the prospects of non-heating LF MF application in enhancing enzyme activity against Gram-negative pathogens.
Development of Novel Paclitaxel-Loaded ZIF-8 Metal-Organic Framework Nanoparticles Modified with Peptide Dimers and an Evaluation of Its Inhibitory Effect against Prostate Cancer Cells
Authors: Zhao Heming , Gong Liming , Wu Hao , Liu Chao , Liu Yanhong , Xiao Congcong , Liu Chenfei , Chen Liqing , Jin Mingji , Gao Zhonggao , Guan Youyan , Huang Wei
Published: July 2023

Prostate cancer (PC) is one of the common malignant tumors of the male genitourinary system. Here, we constructed PTX@ZIF-8, which is a metal-organic-framework-encapsulated drug delivery nanoparticle with paclitaxel (PTX) as a model drug, and further modified the synthesized peptide dimer (Di-PEG2000-COOH) onto the surface of PTX@ZIF-8 to prepare a nanotargeted drug delivery system (Di-PEG@PTX@ZIF-8) for the treatment of prostate cancer. This study investigated the morphology, particle size distribution, zeta potential, drug loading, encapsulation rate, stability, in vitro release behavior, and cytotoxicity of this targeted drug delivery system, and explored the uptake of Di-PEG@PTX@ZIF-8 by human prostate cancer Lncap cells at the in vitro cellular level, as well as the proliferation inhibition and promotion of apoptosis of Lncap cells by the composite nanoparticles. The results suggest that Di-PEG@PTX@ZIF-8, as a zeolitic imidazolate frameworks-8-loaded paclitaxel nanoparticle, has promising potential for the treatment of prostate cancer, which may provide a novel strategy for the delivery system targeting prostate cancer.
Anticancer Tungstenocenes with a Diverse Set of (O,O–), (O,S–) and (O,N–) Chelates—A Detailed Biological Study Using an Improved Evaluation via 3D Spheroid Models
Authors: Cseh Klaudia , Berasaluce Iker , Fuchs Valentin , Banc Alexandra , Schweikert Andreas , Prado-Roller Alexander , Hejl Michaela , Wernitznig Debora , Koellensperger Gunda , Jakupec Michael A. , Kandioller Wolfgang , Malarek Michael S. , Keppler Bernhard K.
Published: July 2023

The synthesis, characterization and biological activity of tungstenocenes with varying biologically active (O,O–), (S,O–) and (N,O–) chelates are described. Complexes were characterized by 1H and 13C NMR, elemental analysis, ESI-mass spectrometry, FT-IR spectroscopy and X-ray diffraction analysis. The aqueous stability was studied by UV/Vis spectroscopy and the WIV to WV process by cyclic voltammetry. The cytotoxicity was determined by the MTT assay in A549, CH1/PA-1 and SW480 cancer cells as well as in IMR-90 human fibroblasts. Extensive biological evaluation was performed in three other human cancer cell lines (HCT116, HT29 and MCF-7) in monolayer and multicellular tumor spheroid cultures to better understand the mode of action. Lead compounds showed promising in vitro anticancer activity in all cancer cell lines. Further studies yielded important insights into apoptosis induction, ROS generation, different patterns in metal distribution (detected by LA-ICP-TOF-MS), changes in KI67 (proliferation marker) expression and DNA interactions. The results based on qualitative and quantitative research designs show that complexes containing (S,O–) chelates are more active than their (O,O–) and (N,O–) counterparts. The most striking results in spheroid models are the high antiproliferative capacity and the different distribution pattern of two complexes differing only in a W–S or W–O bond.
Ciprofloxacin-Loaded Polyvinylpyrrolidone Foils for the Topical Treatment of Wound Infections with Methicillin-Resistant Staphylococcus aureus (MRSA)
Authors: Rancan Fiorenza , Jurisch Jana , Hadam Sabrina , Vogt Annika , Blume-Peytavi Ulrike , Bayer Ilker S. , Contardi Marco , Schaudinn Christoph
Published: July 2023

Bacterial infections are a constant challenge in the management of acute and chronic wounds. Chronic wounds, such as diabetic foot ulcers, have increased significantly in the last few years due to the rise of an aging population. A better understanding of the infectious pathophysiological mechanisms is urgently needed along with new options for the treatment of wound infections and wound-healing disorders. New advances in the preparation of biocompatible dressing materials that can be loaded with antimicrobial drugs may improve the topical treatment of infected wounds. In this study, we investigated the antimicrobial activity of polyvinylpyrrolidone (PVP) foils loaded with ciprofloxacin (Cipro-foils) in the presence of acetic acid as a co-solvent. We used ex vivo human wounds that were infected with two bacterial strains: methicillin-resistant Staphylococcus aureus (MRSA) or Pseudomonas aeruginosa (PAO1). The effectiveness of the treatment was demonstrated by the quantification of the living bacteria extracted from the wound and the detection of released immunological mediators in skin extracts and in the skin culture media. We found that Cipro-foils effectively treated the infection with both PAO1 and MRSA. Other than PAO1, MRSA had no lytic activity toward skin proteins. MRSA infections increased cytokines’ expression and release. Interestingly, treatment with Cipro-foils could partially counteract these effects.
Angle-Dependent Transport Theory-Based Ray Transfer Function for Non-Contact Diffuse Optical Tomographic Imaging
Authors: Kim Stephen Hyunkeol , Jia Jingfei , Hielscher Andreas H.
Published: July 2023

This work presents a generalized angle-dependent ray transfer function that can accurately map the angular and spatial distribution of light intensities on the tissue surface onto a camera image plane in a non-contact camera-based imaging system. The method developed here goes beyond existing ray transfer models that apply to angle-averaged tomographic data alone. The angle-dependent ray transfer operator was constructed using backward ray tracing based on radiation surface theory. The proposed method was validated using numerical phantoms and experimental data from an actual non-contact imaging system.
Post Optical Freeform Compensation Technique for Machining Errors of Large-Aperture Primary Mirror
Authors: Huang Cheng , Li Jinpeng , Cai Zhaohan , Yu Jiadong , Xie Yongjun , Mao Xianglong
Published: July 2023

Large-aperture space optical systems are important tools for observing our planet and conducting deep-space scientific research. More requirements have been put forward for large-aperture optical systems as the depth and breadth of related applications continue to increase. However, achieving the desired surface accuracy on lightweight materials for large-aperture mirror brings higher demands on relevant processing technologies, which increases the processing difficulty, cycle, and cost of large-aperture optical systems. Therefore, it is necessary to develop a new optical system technique with high tolerance for primary mirror machining errors to significantly reduce its machining accuracy requirements. This paper proposes a new optical system technique which introduces a small-aperture free-form surface into the large-aperture optical system’s post optical path. By combining the freeform correction and its misalignment on the system’s aberrations, the system’s wavefront can be adjusted to correct the wavefront distortion caused by the primary mirror’s machining errors. This reduced the machining accuracy requirements of the large-aperture primary mirror by about one order of magnitude, and high-quality imaging with a low-precision primary mirror is achieved.
Copper Sulfide Small Nanoparticles as Efficient Contrast Agent for Photoacoustic Imaging
Authors: Gellini Cristina , Ricci Marilena , Feis Alessandro
Published: July 2023

An experimental study on an innovative contrast agent is presented. This work demonstrates that copper sulfide in the form of small-sized nanoparticles can be exploited in photoacoustic imaging. An advantage of this material is strong light absorption in the near-infrared range, especially in the transparency windows of biological tissues. In order to yield a proper contrast, light absorption must be followed by heat release with high efficiency. Therefore, it is important to evaluate the photochemical conversion efficiency of the material. We applied a method that is strictly related to photoacoustic applications. The nanoparticles were produced according to a well-established synthesis. Subsequently, they were diluted in pure water to obtain an extinction <0.2/cm at 1064 nm. The photoacoustic signals, generated by 1064 nm laser excitation, were analyzed as a function of concentration and incident laser energy below 70 μJ /pulse. The signals were carefully compared with those of a reference aqueous solution, containing a light-absorbing ionic solute. Data analysis yielded a light-to-heat conversion efficiency 1.0 (±0.1). We discuss this result by comparison with related studies on other types of copper sulfide nanoparticles, where the conversion efficiency reportedly varied from 33% to 93%. The high value determined in the present study possibly indicates that resonant light scattering and luminescence are negligible for our material system.
Comparative Evaluation of Antioxidant Status and Mineral Composition of Diploschistes ocellatus, Calvatia candida (rostk.) Hollós, Battarrea phalloides and Artemisia lerchiana in Conditions of High Soil Salinity
Authors: Golubkina Nadezhda , Tolpysheva Tatiana , Lapchenko Vladimir , Lapchenko Helene , Pirogov Nikolay , Zaitsev Viacheslav , Sękara Agnieszka , Tallarita Alessio , Stoleru Vasile , Murariu Otilia Cristina , Caruso Gianluca
Published: July 2023

Natural reserves play a fundamental role in maintaining flora and fauna biodiversity, but the biochemical characteristics of such ecosystems have been studied in an extremely fragmentary way. For the first time, mineral composition and antioxidant status of three systematic groups of organisms, lichens (Diplischistes ocellatus), mushrooms (Calvatia candida and Battarrea phalloides) and wormwood (Artemisia lerchiana) have been described at the territory of Bogdinsko-Baskunchak Nature Reserve (Astrakhan region, Russia), characterized by high salinity and solar radiation, and water deficiency. Through ICP-MS, it was determined that scale lichen D. ocellatus accumulated up to 10–15% Ca, 0.5% Fe, 15 mg kg−1 d.w. iodine (I), 54.5 mg kg−1 Cr. Battarrea phalloides demonstrated anomalously high concentrations of B, Cu, Fe, Mn Se, Zn, Sr and low Na levels, contrary to Calvatia candida mushrooms accumulating up to 10,850 mg kg−1 Na and only 3 mg kg−1 Sr. The peculiarity of A. lerchiana plants was the high accumulation of B (22.23 mg kg−1 d.w.), Mn (57.48 mg kg−1 d.w.), and antioxidants (total antioxidant activity: 68.6 mg GAE g−1 d.w.; polyphenols: 21.0 mg GAE g−1 d.w.; and proline: 5.45 mg g−1 d.w.). Diploschistes ocellatus and Calvatia candida demonstrated the lowest antioxidant status: 3.6–3.8 mg GAE g−1 d.w. total antioxidant activity, 1.73–2.10 mg GAE g−1 d.w. polyphenols and 2.0–5.3 mg g−1 d.w. proline. Overall, according to the elemental analysis of lichen from Baskunchak Nature Reserve compared to the Southern Crimean seashore, the vicinity of Baskunchak Salty Lake elicited increased environmental levels of Cr, Si, Li, Fe, Co, Ni and Ca.
The Growth Oscillator and Plant Stomata: An Open and Shut Case
Authors: Lamport Derek T. A.
Published: July 2023

Since Darwin’s “Power of Movement in Plants” the precise mechanism of oscillatory plant growth remains elusive. Hence the search continues for the hypothetical growth oscillator that regulates a huge range of growth phenomena ranging from circumnutation to pollen tube tip growth and stomatal movements. Oscillators are essentially simple devices with few components. A universal growth oscillator with only four major components became apparent recently with the discovery of a missing component, notably arabinogalactan glycoproteins (AGPs) that store dynamic Ca2+ at the cell surface. Demonstrably, auxin-activated proton pumps, AGPs, Ca2+ channels, and auxin efflux “PIN” proteins, embedded in the plasma membrane, combine to generate cytosolic Ca2+ oscillations that ultimately regulate oscillatory growth: Hechtian adhesion of the plasma membrane to the cell wall and auxin-activated proton pumps trigger the release of dynamic Ca2+ stored in periplasmic AGP monolayers. These four major components represent a molecular PINball machine a strong visual metaphor that also recognises auxin efflux “PIN” proteins as an essential component. Proton “pinballs” dissociate Ca2+ ions bound by paired glucuronic acid residues of AGP glycomodules, hence reassessing the role of proton pumps. It shifts the prevalent paradigm away from the recalcitrant “acid growth” theory that proposes direct action on cell wall properties, with an alternative explanation that connects proton pumps to Ca2+ signalling with dynamic Ca2+ storage by AGPs, auxin transport by auxin-efflux PIN proteins and Ca2+ channels. The extensive Ca2+ signalling literature of plants ignores arabinogalactan proteins (AGPs). Such scepticism leads us to reconsider the validity of the universal growth oscillator proposed here with some exceptions that involve marine plants and perhaps the most complex stress test, stomatal regulation.
The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel
Authors: Zamree Nur Diyanah , Puasa Nurul Aini , Lim Zheng Syuen , Wong Chiew-Yen , Shaharuddin Noor Azmi , Zakaria Nur Nadhirah , Merican Faradina , Convey Peter , Ahmad Syahida , Shaari Hasrizal , Azmi Alyza Azzura , Ahmad Siti Aqlima , Zulkharnain Azham
Published: July 2023

Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degraders. Therefore, this study focused on the utilisation of an Antarctic microalga in the bioremediation of diesel. The studied microalgal strain was originally obtained from a freshwater ecosystem in Paradise Bay, western Antarctic Peninsula. When analysed in systems with and without aeration, this microalgal strain achieved a higher growth rate under aeration. To maintain the growth of this microalga optimally, a conventional one-factor-at a-time (OFAT) analysis was also conducted. Based on the optimized parameters, algal growth and diesel degradation performance was highest at pH 7.5 with 0.5 mg/L NaCl concentration and 0.5 g/L of NaNO3 as a nitrogen source. This currently unidentified microalga flourished in the presence of diesel, with maximum algal cell numbers on day 7 of incubation in the presence of 1% v/v diesel. Chlorophyll a, b and carotenoid contents of the culture were greatest on day 9 of incubation. The diesel degradation achieved was 64.5% of the original concentration after 9 days. Gas chromatography analysis showed the complete mineralisation of C7–C13 hydrocarbon chains. Fourier transform infrared spectroscopy analysis confirmed that strain WCY_AQ5_3 fully degraded the hydrocarbon with bioabsorption of the products. Morphological and molecular analyses suggested that this spherical, single-celled green microalga was a member of the genus Micractinium. The data obtained confirm that this microalga is a suitable candidate for further research into the degradation of diesel in Antarctica.
Effect of Soil and Root Extracts on the Innate Immune Response of American Ginseng (Panax quinquefolius) to Root Rot Caused by Ilyonectria mors-panacis
Authors: Behdarvandi Behrang , Goodwin Paul H.
Published: July 2023

Panax quinquefolius shows much higher mortality to Ilyonectria mors-panacis root rot when grown in soil previously planted with ginseng than in soil not previously planted with ginseng, which is known as ginseng replant disease. Treatment of ginseng roots with methanol extracts of previous ginseng soils significantly increased root lesion sizes due to I. mors-panacis compared to roots treated with water or methanol extracts of ginseng roots or non-ginseng soils. Inoculation of water-treated roots with I. mors-panacis increased expression of a basic chitinase 1 gene (PqChi-1), neutral pathogenesis-related protein 5 gene (PqPR5) and pathogenesis-related protein 10-2 gene (PqPR10-2), which are related to jasmonic acid (JA), ethylene (ET) or necrotrophic infection, and also increased expression of an acidic β-1-3-glucanase gene (PqGlu), which is related to salicylic acid (SA). Infection did not affect expression of a cysteine protease inhibitor gene (PqCPI). Following infection, roots treated with ginseng root extract mostly showed similar expression patterns as roots treated with water, but roots treated with previous ginseng soil extract showed reduced expression of PqChi-1, PqPR5, PqPR10-2 and PqCPI, but increased expression of PqGlu. Methanol-soluble compound(s) in soil previously planted with ginseng are able to increase root lesion size, suppress JA/ET-related gene expression and trigger SA-related gene expression in ginseng roots during I. mors-panacis infection, and may be a factor contributing to ginseng replant disease.
Bisphenol A Diglycidyl Ether-Primary Amine Cooligomer-poly(ε-caprolactone) Networks: Synthesis and Characterization
Authors: Czifrák Katalin , Lakatos Csilla , Szabó Gabriella , Vadkerti Bence , Daróczi Lajos , Zsuga Miklós , Kéki Sándor
Published: July 2023

In this work, the preparation and systematic investigation of cross-linked polyurethane-epoxy (PU-EP) polymer systems are reported. The PU-EP polymers were prepared using a reaction of isocyanate (NCO)-terminated PU-prepolymer with diglycidyl ether of bisphenol A (DGEBA)-amine cooligomer. The oligomerization of DGEBA was carried out by adding furfurylamine (FA) or ethanolamine (EA), resulting in DGEBA-amine cooligomers. For the synthesis of NCO-terminated PU-prepolymer, poly(ε-caprolactone)diol (PCD) (Mn = 2 kg/mol) and 1,6-hexamethylene diisocyanate (HDI) were used. The cross-linking was achieved by adding DGEBA-amine cooligomer to PU-prepolymer, in which the obtained urethane bonds, due to the presence of free hydroxil groups in the activated DGEBA, served as netpoints. During cross-linking, ethanolamine provides an additional free hydroxyl group for the formation of a new urethane bond, while furfurylamine can serve as a thermoreversible coupling element (e.g., Diels–Alder adduct). The PU-EP networks were characterized using attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), dynamical mechanical analysis (DMA) and scanning electron microscopy (SEM). The DMA curves of some PU-EPs (depending on the compositions and the synthetic method) revealed a plateau-like region above the melting temperature (Tm) of PCD, confirming the presence of a cross-linked structure. This property resulted in a shape memory (SM) behavior for these samples, which can be fine-tuned in the presence of furfurylamine through the formation of additional thermoreversible bonds (e.g., Diels–Alder adduct).
The Development of Environmentally Sustainable Poly(vinyl chloride) Composite from Waste Non-Metallic Printed Circuit Board with Interfacial Agents
Authors: Moe Aung Kyaw , Chungprempree Jirasuta , Preechawong Jitima , Sapsrithong Pornsri , Nithitanakul Manit
Published: July 2023

The recycling of non-metallic printed circuit boards (NMPCB) as a filler in poly(vinyl chloride) (PVC) composite would help to encourage the use of waste NMPCB, thus, reducing some environmental concerns with regard to e-waste. The objective of this study was to comprehensively evaluate the effect of different interfacial agents, namely polypropylene grafted maleic anhydride (PP-g-MAH) and ϒ-aminopropyltriethoxy silane (ATPS) on the morphology and properties of PVC/NMPCB composites. A PVC/NMPCB composite was prepared by melt compounding with varying amounts of NMPCB ranging between 10, 20 and 30 wt.%. Fourier transform infrared spectroscopy–attenuated total reflectance (FTIR–ATR) analysis revealed the interactions between PVC and NMPCB when using both PP-g-MAH and ATPS interfacial agent. The properties and morphology of PVC/NMPCB composites were significantly dependent on the interfacial agent treated on the NMPCB surface. The phase morphology and mechanical properties of PVC/NMPCB composites (30 wt.% of NMPCB) were improved and the result also indicated that the higher compatibility of composites with ATPS as an interfacial agent led to our obtaining the maximum Young’s modulus of 484 MPa. The dynamic mechanical analysis revealed the interaction at the interface, with the Tg shifting to a lower temperature in the presence of PP-g-MAH and strong interfacial adhesion noted with the improved Tg in the presence of the ATPS interfacial agent. Further evidence of the improved interaction was observed with the increment in density in the presence of ATPS when compared with PP-g-MAH in PVC/NMPCB composite. Hence, of the two interfacial agents, ATPS showed itself to be more effective when employed as an interfacial agent for NMPCB in PVC composite for industry.
Diving into Fish Valorisation: Review Opportunities and Analyzing Azorean Fish Data
Authors: Valério Nádia , Soares Margarida , Vilarinho Cândida , Correia Manuela , Carvalho Joana
Published: July 2023

In response to the exponential growth in world population, there has been a striking surge in the volume of discarded fish worldwide. This surge is particularly evident in the fish processing industry, where a substantial amount of waste is generated, posing significant environmental concerns. Consequently, the repurposing and utilisation of these waste materials have emerged as pivotal processes for the preservation of marine resources. By employing innovative strategies, valuable products can be extracted from these fish by-products, offering not only economic advantages but also contributing to mitigating environmental impacts. This comprehensive literature review focuses on exploring diverse avenues for using fish waste and extracting high-value materials such as bioactive peptides, collagen, and enzymes, elucidating their potential applications across various industries. The literature review also demonstrates the possibility of extracting various bio-compounds from highly diverse fish waste. It has been observed that there is a need for optimisation of extraction protocols, as the variation in extraction methods and respective conditions significantly affects the extraction yields of the products. Moreover, considering our specific interest in the fish species endemic to The Azores, a meticulous characterisation will be conducted, as there is limited knowledge about waste utilisation processes specific to this archipelago.
Green Supply Chain Circular Economy Evaluation System Based on Industrial Internet of Things and Blockchain Technology under ESG Concept
Authors: Qian Cheng , Gao Yuying , Chen Lifeng
Published: July 2023

A green supply chain economy considering environmental, social, and governance (ESG) factors improves the chances of functional growth through minimal risk factors. The implication of sophisticated technologies such as the Industrial Internet of Things (IIoT) and the blockchain improves the optimization and evaluation of ESG performance. An IIoT-Blockchain-based Supply Chain Economy Evaluation (IB-SCEE) model is introduced to identify and reduce functional growth risk factors. The proposed model uses green blockchain technology to identify distinct transactions’ economic demands and supply distribution. The flaws and demands in the circular economy process are validated using the IIoT forecast systems relying on ESG convenience. The minimal and maximum risks are identified based on economic and distribution outcomes. The present investigation highlights the significance of ongoing ESG-conceptualized research into blockchain-based supply chain economics. Companies who recognize the blockchain’s potential can improve corporate governance, environmental impact, and social good by increasing transparency, traceability, and accountability. A more sustainable and responsible future for global supply chains can be shaped through further research and development in this field, which will make a substantial contribution to the scientific world. This information is individually held in the green blockchain for individual risk factor analysis. The proposed model improves the recommendation and evaluation rate and reduces the risk factors with controlled evaluation time.
Characteristics and Geological Impact Factors of Coalbed Methane Production in the Taiyuan Formation of the Gujiao Block
Authors: Wang Gang , Xie Yiwei , Chang Huizhen , Du Liqiang , Wang Qi , He Tao , Zhang Shuaiyi
Published: July 2023

The coalbed methane resources of the Gujiao Block are abundant, and the exploration degree is high. The gas production and water production of different CBM (coalbed methane) wells vary greatly. The average gas production of CBM wells in the study area is mostly less than 1000 m3/d, while the average water production is mostly less than 5 m3/d. The gas production of CBM wells near the core of the Malan syncline is relatively high. A series of large faults exist in the central and eastern parts of the study area, and CBM wells nearby produce more water but less gas. The salinity of water discharged from CBM wells ranges from 810.34 to 3115.48 mg/L, which is consistent with the trend of a gradual increase from north to south. The gas content distribution follows this same gradually increasing north to south trend. Coal thickness and buried depth have little effect on gas production, but have some effects on water production. The endogenous fracture system in the coal reservoir is extremely developed and the porosity and permeability of the reservoirs are low, which is not conducive to the migration and recovery of coalbed methane. The adsorption capacity of the coal sample is strong. However, the continuous uplift and denudation of the stratum from the middle Yanshanian to the Himalayan region are not conducive to the preservation and enrichment of coalbed methane. In addition, a series of large faults exist in this area, and the coal structures are broken. A large amount of coalbed methane is continually being released. Generally, structural and hydrological conditions affect the porosity, permeability, and gas content of coal reservoirs, thereby affecting the productivity of coalbed methane wells. The comprehensive analysis shows that the Xingjiashe well field in the southern part of the study area is a favorable area for CBM exploration and development.
A New Fast Calculating Method for Meshing Stiffness of Faulty Gears Based on Loaded Tooth Contact Analysis
Authors: Liu Zhe , Wang Haiwei , Lu Fengxia , Wang Cheng , Zhang Jiachi , Qin Mingjian
Published: July 2023

Gear transmission systems are widely used in various fields. The occurrence of gear cracks, tooth pitting, and other faults will lead to the dynamic characteristics deterioration of the transmission system. In order to calculate the meshing stiffness of faulty gear pairs more effectively and precisely, this article improves the loaded tooth contact analysis (LTCA) method by analyzing the influence of different fault types on gear deformation, including bending-shearing deformation and contact deformation, which combines the accuracy of the finite element method (FEM) and the rapidity of the analytical method (AM). The improved LTCA method can model the fault areas accurately and optimize the deformation coordination equation under the actual meshing situation of the faulty gear tooth, making it suitable for calculating the meshing stiffness of faulty gears. Based on the calculation results of the finite element method, the accuracy of the improved meshing stiffness calculation method has been verified, and the sensitivity of different fault type parameters on meshing stiffness has been studied.
Deciphering the Inscriptions on “The Adoration of the Magi” by Bartholomäus Zeitblom on the High Altar of Blaubeuren
Authors: Jara-Vera Vicente , Sánchez-Ávila Carmen
Published: July 2023

The Blaubeuren monastery (Germany) is home to one of the most beautiful religious artistic expressions of Swabian art from the end of the 15th century, a collaborative work of various artists and workshops, including Jörg Syrlin the Younger, Michel Erhart, Hans Schüchlin, Bartholomäus Zeitblom, and Bernhard Strigel. It is a high altar triptych with a double opening. On its right inner bas-relief is “The Adoration of the Magi”, a work by Bartholomäus Zeitblom (ca. 1455–1520), a prominent member of the Ulm School. In the scene, on King Balthazar’s leg, there is a short literary text and a number, “DIER ZVO LIEB 100”, as well as a crowned monogram among floral elements. In this decipherment study, we propose meanings for all of these inscriptions, based on a collection of various previous solutions. The conclusions lead us to a pious expression toward the Child God based on an old Tyrolean Christmas carol compiled in the 19th century, which we trace back to at least the 15th century, an author’s signature by Zeitblom, and an acknowledgment of the royalty of the Virgin Mother Mary.
Solidarity as a Challenge and a Task
Authors: Jagiełło Jarosław
Published: July 2023

Towards the end of the 20th century, the word “solidarity” became one of the most important and famous words, not only in the sphere of Euro–Atlantic civilization but the word was also readily used in political milieus. In the religious sphere, and especially in the Judeo–Christian tradition, the anthropological, ethical as well as biblical sense of this important concept was emphasized. This sense was recalled in the postulate: Bear one another’s burdens. Never one against the other, but always one and the other, one together with the other. In this day and age, solidarity as a source of inter-human hope poses a real challenge and task for us because we are experiencing such great migrations of people who—for a variety of reasons, frequently very painful ones, and among them the devastating ravages of war—leave behind their places of residence and go into exile. In my study, I will try to show how, in the contemporary world of philosophy and socio-political changes, a modern sense of the concept of “solidarity” has been generated. In order to achieve this goal, I will refer to the anthropological and ethical thoughts of two Polish philosophers—Karol Wojtyła and Józef Tischner. It was these two thinkers who introduced us to the contemporary school of solidarity, reminding us that the fulfillment of solidarity still lies ahead of us.
An Open PLC-Based Robot Control System for 3D Concrete Printing
Authors: Kajzr Daniel , Myslivec Tomas , Cernohorsky Josef
Published: July 2023

Three-dimensional concrete printing technology is currently a very topical and developing subject. There is a large number of applications worldwide where this technology can be used. In connection with this technology, the development of custom industrial robotic systems and their control is essential. Conventional closed-loop control system platforms do not provide sufficiently flexible solutions. This paper presents a control system for a unique printing robot that, thanks to its openness and unified platform, will enable simple and fast analysis and testing of key aspects in terms of control and guidance of the printing robot for additive manufacturing applications in the construction industry. The aim of this paper is to introduce the concept of an open PLC-based control system and to demonstrate its usefulness in the task of designing and implementing model-based control. All steps, from the analysis of the printing robot itself and identification of inertial parameters to the actual design and implementation of the control, can be executed in a unified Matlab/Simulink environment using various add-ons and toolboxes thanks to the open control system platform. This solution brings significant savings in terms of programming and prototyping time. The open control system was used to control an experimental model of a printing robot, serving as a test bed for the final version of the printing robot, and the results obtained were evaluated.
PVL-Cartographer: Panoramic Vision-Aided LiDAR Cartographer-Based SLAM for Maverick Mobile Mapping System
Authors: Zhang Yujia , Kang Jungwon , Sohn Gunho
Published: July 2023

The Mobile Mapping System (MMS) plays a crucial role in generating accurate 3D maps for a wide range of applications. However, traditional MMS that utilizes tilted LiDAR (light detection and ranging) faces limitations in capturing comprehensive environmental data. We propose the “PVL-Cartographer” SLAM (Simultaneous Localization And Mapping) approach for MMS to address these limitations. This proposed system incorporates multiple sensors to yield dependable and precise mapping and localization. It consists of two subsystems: early fusion and intermediate fusion. In early fusion, range maps are created from LiDAR points within a panoramic image space, simplifying the integration of visual features. The SLAM system accommodates both visual features with and without augmented ranges. In intermediate fusion, camera and LiDAR nodes are merged using a pose graph, with constraints between nodes derived from IMU (Inertial Measurement Unit) data. Comprehensive testing in challenging outdoor settings demonstrates that the proposed SLAM system can generate trustworthy outcomes even in feature-scarce environments. Ultimately, our suggested PVL-Cartographer system effectively and accurately addresses the MMS localization and mapping challenge.