bluuebunny/crossref_metadata_2025 huggingface dataset:
BENTUK BAHASA GAUL PADA KOMENTAR STATUS DI MEDIA SOSIAL FACEBOOK
Authors: Mayangsari Wa Ode Sitti , Lindayani Lilik Rita
Published: September 2024

Bentuk Bahasa Gaul pada Komentar Status di Media Sosial Facebook, oleh Wa Ode Sitti Mayangsari. Judul ini dipilih dengan tujuan untuk menemukan dan mendeskripsikan bentuk bahasa gaul  yang terdapat dalam media sosial facebook, tepatnya pada grub Meme Indonesia. Subjek dalam penelitian ini adalah komentar status pada grub Meme Indonesia, dalam penelitian ini mengambil jumlah data sebanyak 40 data.  Penelitian ini merupakan penelitian deskriptif  kualitatif. Data penelitian merupakan data tertulis berupa ujaran atau tuturan yang terdapat pada komentar status pada grub Meme Indonesia di media facebook. Data diperoleh dengan menggunakan teknik baca, catat, dan dokumentasi. Analisis data dilakukan dengan menggunakan pendekatan sosiolinguistik. Hasil penelitian menunjukkan bahwa bentuk bahasa gaul pada komentar status di media sosial facebook, terdapat beberapa bentuk yaitu penggabungan, potongan, akronim, singkatan dan metatesis yang terdapat dalam interaksi di media sosial facebook pada grub Meme Indonesia berdasarkan konteksnya.
Kata kunci : Bentuk Bahasa Gaul, Media Sosial, Facebook.
A systematic review of the impact of therapeutical biophilic design on health and wellbeing of patients and care providers in healthcare services settings
Authors: Al Khatib Inas , Samara Fatin , Ndiaye Malick
Published: September 2024

Hospitals often evoke negative feelings due to their antiseptic architecture and personal memories. Biophilic hospital design can reduce stress and enhance health and wellness for patients and caregivers, creating sustainable therapeutic environments. This research paper explores these environments and presents case studies demonstrating the positive outcomes of biophilic design on health and wellbeing. This paper systematically reviews and critically assesses literature using secondary data from peer-reviewed journals and reliable sources on sustainable design from 2010–2023. The results show that biophilic design in hospitals reduces hospitalization time, patient mortality, pain levels, and stress for healthcare providers. It alleviates anxiety, improves experiences for patients, families, and staff, reduces patient harm, and supports faster recovery. Overall, it positively influences the psychological and physiological responses of patients and staff. Future research should analyze the impact of individual biophilic design elements separately and explore implementation challenges. It should also quantify benefits such as reduced staff absenteeism and increased productivity. This study fills the gap of the limitation of holistic studies on biophilic design’s impact on patient care and service delivery in hospital settings.
New molecular mechanisms of quercetin in improving recurrent spontaneous abortion based on in-depth network pharmacology and molecular docking
Authors: Wang Dan , Li Xuebing , Li Yifan , Wang Ruilin , Wang Chunxia , Li Yongwei
Published: September 2024

Introduction

The increasing prevalence of recurrent spontaneous abortion (RSA) poses significant physical and psychological challenges for affected individuals. Quercetin, a natural plant flavonoid, shows promise in reducing miscarriage rates, yet its precise mechanism remains elusive. This study uses network pharmacology, molecular docking, and experimental validation to explore the molecular pathways through which quercetin mitigates RSA.

Methods

Quercetin-related target genes were sourced from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), and RSA target genes were retrieved from the Comparative Toxicogenomics Database (CTD), with overlapping targets identified using Venn diagrams. All genes were visualized using the STRING database, and core targets were selected with Cytoscape 3.7.3. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted using the DAVID and Reactome online resources. Subsequently, HTR-8/SVneo cells were stimulated with lipopolysaccharide (LPS) and treated with varying concentrations of quercetin (1, 5, and 10 μM), then subjected to CCK-8, wound healing, transwell, and annexin V-FITC/PI apoptosis assays. Reverse-transcription quantitative PCR was used to determine the mRNA expression levels of IL-1β, TNF-α, and IL-6 in LPS-induced cells post-quercetin intervention, and western blotting was used to measure AKT1, MMP9, and caspase-3 protein levels.

Results

A total of 139 quercetin-associated target genes were identified from the TCMSP database, and 98 disease-associated target genes were obtained from the CTD, resulting in 25 shared target genes. Gene ontology enrichment highlighted the involvement of these targets in positive regulation of apoptosis, response to hypoxia, and intrinsic apoptotic signaling pathway in response to DNA damage. KEGG pathway analysis indicated enrichment in pathways related to interleukin-4 and interleukin-13 signaling, cytokine signaling in the immune system, and apoptosis. Molecular docking studies revealed robust binding of quercetin with MMP9, AKT1, IL-1β, TNF, and caspase-3. In vitro experiments demonstrated that quercetin enhanced LPS-induced cell activity, fostering proliferation, migration, and invasion, and reducing apoptosis. Moreover, quercetin reduced IL-1β, TNF-α, and IL-6 mRNA expression, increased AKT1 and MMP9 protein levels, and reduced caspase-3 expression.

Conclusion

Quercetin could mitigate the incidence of RSA by modulating inflammatory responses and apoptotic processes, through upregulation of AKT1 and MMP9, and downregulation of caspase-3, IL-1β, TNF-α, and IL-6. Quercetin opens up a new way of thinking about treating RSA.
Individual differences in visuo-spatial working memory capacity and prior knowledge during interrupted reading
Authors: Zermiani Francesca , Dhar Prajit , Strohm Florian , Baumbach Sibylle , Bulling Andreas , Wirzberger Maria
Published: September 2024

Interruptions are often pervasive and require attentional shifts from the primary task. Limited data are available on the factors influencing individuals' efficiency in resuming from interruptions during digital reading. The reported investigation—conducted using the InteRead dataset—examined whether individual differences in visuo-spatial working memory capacity (vsWMC) and prior knowledge could influence resumption lag times during interrupted reading. Participants' vsWMC capacity was assessed using the symmetry span (SSPAN) task, while a pre-test questionnaire targeted their background knowledge about the text. While reading an extract from a Sherlock Holmes story, they were interrupted six times and asked to answer an opinion question. Our analyses revealed that the interaction between vsWMC and prior knowledge significantly predicted the time needed to resume reading following an interruption. The results from our analyses are discussed in relation to theoretical frameworks of task resumption and current research in the field.
Accuracy and role of consumer facing wearable technology for continuous monitoring during endoscopic procedures
Authors: AbiMansour Jad P. , Kaur Jyotroop , Velaga Saran , Vatsavayi Priyanka , Vogt Matthew , Chandrasekhara Vinay
Published: September 2024

Background

Consumer facing wearable devices capture significant amounts of biometric data. The primary aim of this study is to determine the accuracy of consumer-facing wearable technology for continuous monitoring compared to standard anesthesia monitoring during endoscopic procedures. Secondary aims were to assess patient and provider perceptions of these devices in clinical settings.

Methods

Patients undergoing endoscopy with anesthesia support from June 2021 to June 2022 were provided a smartwatch (Apple Watch Series 7, Apple Inc., Cupertino, CA) and accessories including continuous ECG monitor and pulse oximeter (Qardio Inc., San Francisco, CA) for the duration of their procedure. Vital sign data from the wearable devices was compared to in-room anesthesia monitors. Concordance with anesthesia monitoring was assessed with interclass correlation coefficients (ICC). Surveys were then distributed to patients and clinicians to assess patient and provider preferences regarding the use of the wearable devices during procedures.

Results

292 unique procedures were enrolled with a median anesthesia duration of 34 min (IQR 25–47). High fidelity readings were successfully recorded with wearable devices for heart rate in 279 (95.5%) cases, oxygen in 203 (69.5%), and respiratory rate in 154 (52.7%). ICCs for watch and accessories were 0.54 (95% CI 0.46–0.62) for tachycardia, 0.03 (95% CI 0–0.14) for bradycardia, and 0.33 (0.22–0.43) for oxygen desaturation. Patients generally felt the devices were more accurate (56.3% vs. 20.0% agree, p < 0.001) and more permissible (53.9% vs. 33.3% agree, p < 0.001) to wear during a procedure than providers.

Conclusion

Smartwatches perform poorly for continuous data collection compared to gold standard anesthesia monitoring. Refinement in software development is required if these devices are to be used for continuous, intensive vital sign monitoring.
Equitable education for migrant students? Investigating the educational success of newly arrived migrants in Flanders
Authors: Seynhaeve Shauny , Vanbuel Marieke , Kavadias Dimokritos , Deygers Bart
Published: September 2024

Introduction

Previous research has uncovered various inequalities for immigrant students in education systems worldwide. Yet, the degree to which diverse background factors, such as socio-economic status and prior educational trajectory, contribute to these educational disadvantages remains uncertain.

Methods

Using data from the Flemish administrative database (2009–2019) on 48,340 mainstream students and 1,022 Newly Arrived Migrant Students (NAMS) across 685 schools, this study examines NAMS' passing and dropout rates in Flemish secondary education in comparison to the outcomes of their peers without a recent migration background. Data included demographic background variables, school variables, and indicators of educational outcomes.

Results

A cross-classified multilevel regression analysis showed that, after controlling for background variables, NAMS still show higher probabilities of failing a year and of dropping out. Moreover, the impact of SES is stronger for native students than for NAMS.

Discussion

These results suggest the existence of a migrant background effect disadvantaging NAMS in Flemish secondary education.
Influencing factors for postpartum depression in women with gestational diabetes mellitus
Authors: Tan Jinfeng , Xiong Yujing , Wang Xiaohui , Wei Shiyao , Luo Chunqi , Huang Shaoming , Yang Yan , Chen Jinxia , Chen Jiebing , Xu Min , Wu Fengchun
Published: September 2024

Objective

It remains undefined about the association between gestational diabetes mellitus (GDM) and postpartum depression (PPD). Hence, a cross-sectional study was conducted to evaluate the association between GDM and PPD among pregnant women and to investigate the influencing factors for PPD.

Methods

From June 2021 to June 2022, 205 parturients with GDM and 201 without GDM were included in the study as the GDM group and the control group, respectively. The collected data from the general information questionnaire and Self Rating Depression Scale (SDS) were statistically analyzed based on binomial logistic regression analyses and generalized linear mixed models (GLMMs).

Results

Age at delivery, gestational age, glycosylated hemoglobin, triglyceride, SDS, and proportions of women who had a history of induced abortion or GDM were significantly different between the GDM group and control group (P<0.05). The incidence of PPD in the GDM group was significantly higher than that in the control group. The neonatal body weight and triglyceride in GDM women with PPD were significantly lower than those in GDM women without PPD (P<0.001). The univariate logistic regression analysis demonstrated that educational age was a protective factor, while glycosylated hemoglobin and GDM were risk factors for PPD. The multiple linear regression analysis revealed that neonatal body weight (OR=-0.904, 95%CI: -1.657 to -0.152, P=0.019) and educational age (OR=-0.166, 95%CI: -0.306 to -0.025, P=0.021) were protective factor, while GDM (OR=1.854, 95%CI: 1.027-2.681, P<0.0001) was a risk factor for PPD.

Conclusion

GDM may be associated with PPD. Neonatal body weight and educational age were protective factors for PPD, and GDM was a risk factor for PPD. Therefore, more attention should be paid to the mental health status of women with GDM, especially those with lesser educational age and lower neonatal body weight.
Busbar fault diagnosis method based on multi-source information fusion
Authors: Jiang Xuebao , Cao Haiou , Zhou Chenbin , Ren Xuchao , Shen Jiaoxiao , Yu Jiayan
Published: September 2024

Against the backdrop of smart grid development, the electric power system demands higher accuracy and comprehensiveness in fault analysis. Establishing a digital twin platform for multiple equipment faults represents the future direction of power system development. Presently, while many researchers employ artificial intelligence algorithms to diagnose faults in key equipment such as transmission lines and transformers, intelligent diagnostic methods for busbar faults remain insufficient. Therefore, this paper proposes a busbar fault diagnosis method based on multi-source information fusion. Initially, the diagnostic method for busbar faults is explored, conducting both time-domain and frequency-domain analyses on simulated fault data. The data of this model are optimized using Dempster-Shafer evidence theory to enhance algorithm training speed. Subsequently, BP neural network training is implemented. Finally, validation testing of fault data demonstrates a fault recognition accuracy of 99.1% for this method. Experimental results illustrate the method’s feasibility and low computational costs, thereby advancing the development of digital twin platforms for power system fault diagnosis.
A first glimpse into the biogeographic affinities of the shallow benthic communities from the sub-Antarctic Crozet archipelago
Authors: Jossart Quentin , Lelièvre Yann , Kelch Andreas , Figuerola Blanca , Moreau Camille V. E. , Di Franco Davide , Maxwell Jamie , Verheye Marie L. , Mackenzie Melanie , Downey Rachel , Rosenfeld Sebastián , Hourdez Stéphane , Saucède Thomas
Published: September 2024

Sub-Antarctic islands are expected to show a high degree of endemicity due to their remoteness. However, biogeographic affinities in the sub-Antarctic remain poorly understood, especially in the marine realm. Sub-Antarctic islands being at the crossroads between Antarctic and cold temperate regions, biodiversity characterization and biogeographic analyses are a priority for monitoring and rapidly assessing variations associated with environmental changes. One underexplored sub-Antarctic area is Crozet, a protected archipelago located halfway between Antarctica and South Africa. In this study, we investigated the shallow-water Crozet macrofaunal diversity, distribution patterns and biogeographic affinities based on the examination of fieldwork specimens via a thorough morphological identification and a genetic characterisation. The resulting dataset provides an important baseline for further studies and conservation strategies, compiling the first genetic and taxonomic database for the Crozet archipelago. In total, 100 morphotypes were found, belonging to nine different phyla, among which arthropods (32), molluscs (18) and echinoderms (17) were the richest. Forty-seven morphotypes were identified to the species level, among which 20 were reported in Crozet for the first time. This confirms that Crozet is a poorly known region, even compared to other sub-Antarctic areas. A large proportion of species (62%) had circum Southern Ocean or circum sub-Antarctic distributions. These species were mostly shared with Kerguelen (72%), the Magellan Province (64%), and Prince Edward Islands (64%), confirming the patterns found in macroalgae and specific macrofaunal groups. However, this large-distribution statement needs to be counterbalanced by the detection (genetic data) of more restricted distributions than expected in four study cases (the tanaid Apseudes spectabilis, the nudibranch Doris kerguelenensis, the polychaete Neanthes kerguelensis and the chiton Hemiarthrum setulosum). Considering that most morphotypes had no genetic data available from other regions, the proportion of morphotypes with restricted distribution is likely to increase alongside future investigations. In addition, we also found a few cases of unrecognized diversity that might lead to the descriptions of new species, some likely to be endemic to Crozet (e.g., within the polychaete genus Harmothoe and the bryozoan genus Antarctothoa). Altogether, this stresses the need to maintain conservation efforts in Crozet and pursue integrative investigations in order to highlight and protect its unusual diversity.
Identification and validation of diagnostic genes associated with neutrophil extracellular traps of type 2 diabetes mellitus
Authors: He Meifang , Niu Jin , Cheng Haihua , Guo Chaoying
Published: September 2024

Background

Neutrophil extracellular traps (NETs) cause delayed wound closed up in type 2 diabetes mellitus (T2DM), but the specific regulatory mechanism of NETs-related genes (NETs-RGs) in T2DM is unclear.

Methods

We acquired GSE21321 and GSE15932 datasets from gene expression omnibus (GEO) database. First, differentially expressed genes (DEGs) between T2DM and control samples of GSE21321 dataset were sifted out by differential expression analysis. NETs scores were calculated for all samples in GSE21321 dataset, and key module genes associated with NETs scores were screened by constructing co-expression network. Then, DEGs and key module genes were intersected to yield intersection genes, and candidate genes were identified by constructing a protein protein interaction (PPI) network. Least absolute shrinkage and selection operator (LASSO) regression analysis was implemented on candidate genes to screen out diagnostic genes, and they were subjected to single sample gene set enrichment analysis (ssGSEA). Finally, immune characteristic analysis was carried out, and we constructed the gene-drug and transcription factor (TF)-miRNA-mRNA networks. Besides, we validated the expression of diagnostic genes by quantitative real-time polymerase chain reaction (qRT-PCR).

Results

In total, 23 candidate genes were gained by PPI analysis. The 5 diagnostic genes, namely, inter-trypsin inhibitor heavy chain 3 (ITIH3), fibroblast growth factor 1 (FGF1), neuron cell adhesion molecule (NRCAM), advanced glycosylation end-product-specific receptor (AGER), and calcium voltage-gated channel subunit alpha1 C (CACNA1C), were identified via LASSO analysis, and they were involved in carboxylic acid transport, axonogenesis, etc. M2 Macrophage, Monocyte, Natural killer (NK) cell, and Myeloid dendritic cells (DC) were remarkably different between T2DM and control samples. Diagnostic genes had the strongest and the most significant positive correlation with B cells. The gene-drug network included CACNA1C-Isradipine, CACNA1C-Benidipine and other relationship pairs. Totally 76 nodes and 44 edges constituted the TF-miRNA-mRNA network, including signal transducer and activator of transcription 1(STAT1) -hsa-miR-3170-AGER, CCCTC-binding factor (CTCF)-hsa-miR-455-5p-CACNA1C, etc. Moreover, qRT-PCR suggested that the expression trends of FGF1 and AGER were in keeping with the results of bioinformatic analysis. FGF1 and AGER were markedly regulated downwards in the T2DM group.

Conclusion

Through bioinformatic analysis, we identified NETs-related diagnostic genes (ITIH3, FGF1, NRCAM, AGER, CACNA1C) in T2DM, and explored their mechanism of action from different aspects, providing new ideas for the studies related to diagnosis and treatment of T2DM.
Genomic insight into COVID-19 severity in MAFLD patients: a single-center prospective cohort study
Authors: Buchynskyi Mykhailo , Oksenych Valentyn , Kamyshna Iryna , Budarna Olena , Halabitska Iryna , Petakh Pavlo , Kamyshnyi Oleksandr
Published: September 2024

This study investigated the influence of single nucleotide polymorphisms (SNPs) in genes associated with the interferon pathway (IFNAR2 rs2236757), antiviral response (OAS1 rs10774671, OAS3 rs10735079), and viral entry (ACE2 rs2074192) on COVID-19 severity and their association with nonalcoholic fatty liver disease (MAFLD). We did not observe a significant association between the investigated SNPs and COVID-19 severity. While the IFNAR2 rs2236757 A allele was correlated with higher creatinine levels upon admission and the G allele was correlated with lower band neutrophils upon discharge, these findings require further investigation. The distribution of OAS gene polymorphisms (rs10774671 and rs10735079) did not differ between MAFLD patients and non-MAFLD patients. Our study population’s distribution of ACE2 rs2074192 genotypes and alleles differed from that of the European reference population. Overall, our findings suggest that these specific SNPs may not be major contributors to COVID-19 severity in our patient population, highlighting the potential role of other genetic factors and environmental influences.
Microglia in physiological conditions and the importance of understanding their homeostatic functions in the arcuate nucleus
Authors: Guzmán-Ruíz Mara A. , Guerrero Vargas Natalí N. , Ramírez-Carreto Ricardo Jair , González-Orozco Juan Carlos , Torres-Hernández Bryan Adair , Valle-Rodríguez Michelle , Guevara-Guzmán Rosalinda , Chavarría Anahí
Published: September 2024

Microglia are highly dynamic cells that have been mainly studied under pathological conditions. The present review discusses the possible implication of microglia as modulators of neuronal electrical responses in physiological conditions and hypothesizes how these cells might modulate hypothalamic circuits in health and during obesity. Microglial cells studied under physiological conditions are highly diverse, depending on the developmental stage and brain region. The evidence also suggests that neuronal electrical activity modulates microglial motility to control neuronal excitability. Additionally, we show that the expression of genes associated with neuron-microglia interaction is down-regulated in obese mice compared to control-fed mice, suggesting an alteration in the contact-dependent mechanisms that sustain hypothalamic arcuate-median eminence neuronal function. We also discuss the possible implication of microglial-derived signals for the excitability of hypothalamic neurons during homeostasis and obesity. This review emphasizes the importance of studying the physiological interplay between microglia and neurons to maintain proper neuronal circuit function. It aims to elucidate how disruptions in the normal activities of microglia can adversely affect neuronal health.
Developing the script “degenerate primer 111” to enhance the coverage of universal primers for the small subunit rRNA gene on target microorganisms
Authors: Qin Zhihui , Xu Xin , Xu Fengjun , Zhang Yao , Su Peng , Shen Chaofeng
Published: September 2024

Amplifying small subunit (SSU) rRNA genes with universal primers in assessing microbial populations diversity, but target microorganisms are sometimes omitted due to inadequate primer coverage. Adding degenerate bases to primers can help, but existing methods are complex and time-consuming. This study introduces a user-friendly tool called “Degenerate primer 111” for adding degenerate bases to existing universal primers. By aligning one universal primer with one uncovered target microorganism’s SSU rRNA gene, this tool iteratively generates a new primer, maximizing coverage for the target microorganisms. The tool was used to modify eight pairs of universal primers (515F Parada–806R Apprill, S-D-Bact-0341-b-S-17/S-D-Bact-0785-a-A-21, OP_F114-KP_R013, 27F-1492R, 341F-806R, OP_F066-KP_R013, 515F Parada–926R Quince, 616*F-1132R), and generated 29 new universal primers with increased coverage of specific target microorganisms without increasing coverage of non-target microorganisms. To verify the effectiveness of the improved primers, one set of original and improved primers (BA-515F-806R and BA-515F-806R-M1) was used to amplify DNA from the same sample, and high-throughput sequencing of the amplicons confirmed that the improved primers detected more microbial species compared to the original primers. Future researchers can use this tool to develop more personalized primers to meet their diverse microorganism detection needs.
Nitrous oxide production and consumption by marine ammonia-oxidizing archaea under oxygen depletion
Authors: Hernández-Magaña Elisa , Kraft Beate
Published: September 2024

Ammonia-oxidizing archaea (AOA) are key players in the nitrogen cycle and among the most abundant microorganisms in the ocean, thriving even in oxygen-depleted ecosystems. AOA produce the greenhouse gas nitrous oxide (N2O) as a byproduct of ammonia oxidation. Additionally, the recent discovery of a nitric oxide dismutation pathway in the AOA isolate Nitrosopumilus maritimus points toward other N2O production and consumption pathways in AOA. AOA that perform NO dismutation when exposed to oxygen depletion, produce oxygen and dinitrogen as final products. Based on the transient accumulation of N2O coupled with oxygen accumulation, N2O has been proposed as an intermediate in this novel archaeal pathway. In this study, we spiked N2O to oxygen-depleted incubations with pure cultures of two marine AOA isolates that were performing NO dismutation. By using combinations of N compounds with different isotopic signatures (15NO2− pool +44N2O spike and 14NO2− pool +46N2O spike), we evaluated the N2O spike effects on the production of oxygen and the isotopic signature of N2 and N2O. The experiments confirmed that N2O is an intermediate in NO dismutation by AOA, distinguishing it from similar pathways in other microbial clades. Furthermore, we showed that AOA rapidly reduce high concentrations of spiked N2O to N2. These findings advance our understanding of microbial N2O production and consumption in oxygen-depleted settings and highlight AOA as potentially important key players in N2O turnover.
High casein concentration induces diarrhea through mTOR signal pathway inhibition in post-weaning piglets
Authors: Gao Jing , Ma Li , Yin Yulong , Chen Yongzhong , Li Tiejun
Published: September 2024

Weaning is one of the most challenging periods in a pig’s life, during which piglets suffer from nutrition and other issues. Post-weaning diarrhea is one of the major health problems in the pig industry, leading to high morbidity and mortality rates. Previous studies have demonstrated that both the source and concentration of proteins are closely associated with post-weaning diarrhea in piglets. This study was conducted to prevent and control post-weaning diarrhea by selecting different dietary protein concentrations. To eliminate interference from other protein sources, casein was used as the only protein source in this study. Fourteen piglets (weighing 8.43 ± 0.3 kg, weaned on the 28th day) were randomly assigned to two dietary protein groups: a low-protein group (LP, containing 17% casein) and a high-protein group (HP, containing 30% casein). The experiment lasted 2 weeks, during which all piglets had ad libitum access to food and water. Diarrhea was scored on a scale from 1 to 3 (where 1 indicates normal stools and 3 indicates watery diarrhea), and growth performance measurements were recorded daily. The results showed that the piglets in the HP group had persistent diarrhea during the whole study, whereas no diarrhea was observed among piglets in the control group. The body weights and feed intake were significantly lower in piglets in the HP group compared to those in the LP group (p < 0.05). The gastrointestinal pH was significantly higher in piglets in the HP group than those in the LP group (p < 0.05). The intestinal tract microorganisms of the piglets in both groups were significantly affected by the protein concentration of the diet. A diet with high casein concentration significantly reduced the microbiota diversity. Compared to the LP group, the 30% casein diet decreased the abundance of Firmicutes, Bacteroidetes, and Actinobacteria at the phylum level and the relative abundance of Ruminococcus at the genus level. Diarrhea-related mRNA abundances were analyzed by the real-time polymerase chain reaction (PCR) in the intestine of piglets, and the results showed that the HP concentration markedly decreased the expression of solute carriers (SLC, p < 0.05). The mammalian target of rapamycin-mTOR signaling pathway (p < 0.01) was activated in the HP group. In conclusion, a high-protein diet induced post-weaning diarrhea, decreased growth performance, increased gastrointestinal pH, and reduced expression of solute carrier proteins. However, the relationship between high dietary casein feed and post-weaning diarrhea remains unclear and needs to be explored further.
The effects of iron deficient and high iron diets on SARS-CoV-2 lung infection and disease
Authors: Carolin Agnes , Frazer David , Yan Kexin , Bishop Cameron R. , Tang Bing , Nguyen Wilson , Helman Sheridan L. , Horvat Jay , Larcher Thibaut , Rawle Daniel J. , Suhrbier Andreas
Published: September 2024

Introduction

The severity of Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is often dictated by a range of comorbidities. A considerable literature suggests iron deficiency and iron overload may contribute to increased infection, inflammation and disease severity, although direct causal relationships have been difficult to establish.

Methods

Here we generate iron deficient and iron loaded C57BL/6 J mice by feeding standard low and high iron diets, with mice on a normal iron diet representing controls. All mice were infected with a primary SARS-CoV-2 omicron XBB isolate and lung inflammatory responses were analyzed by histology, immunohistochemistry and RNA-Seq.

Results

Compared with controls, iron deficient mice showed no significant changes in lung viral loads or histopathology, whereas, iron loaded mice showed slightly, but significantly, reduced lung viral loads and histopathology. Transcriptional changes were modest, but illustrated widespread dysregulation of inflammation signatures for both iron deficient vs. controls, and iron loaded vs. controls. Some of these changes could be associated with detrimental outcomes, whereas others would be viewed as beneficial.

Discussion

Diet-associated iron deficiency or overload thus induced modest modulations of inflammatory signatures, but no significant histopathologically detectable disease exacerbations.
Deciphering the impact of exogenous fatty acids on Listeria monocytogenes at low temperature by transcriptome analysis
Authors: Quilleré Aurore , Darsonval Maud , Papadochristopoulos Angelos , Amoros Alban , Nicolas Pierre , Dubois-Brissonnet Florence
Published: September 2024

Listeria monocytogenes is a ubiquitous and psychrotrophic foodborne pathogen commonly found in raw materials, ready-to-eat products, and food environments. We previously demonstrated that L. monocytogenes can grow faster at low temperature when unsaturated fatty acids (UFA) are present in its environment. This could question the maintenance of food safety for refrigerated foods, especially those reformulated with a higher ratio of UFA versus saturated fatty acids (SFA) to fit with nutritional recommendations. In this study, we used transcriptomics to understand the impact of UFA on the behavior of L. monocytogenes at low temperature. We first demonstrated that fabK, a key gene in SFA synthesis, is up-regulated in the presence of UFA but not SFA at low temperature. L. monocytogenes can thus regulate the synthesis of SFA in its membrane according to the type of FA available in its environment. Interestingly, we also observed up-regulation of genes involved in chemotaxis and flagellar assembly (especially cheY and flaA) in the presence of UFA but not SFA at low temperature. TEM observations confirmed that L. monocytogenes acquired a remarkable phenotype with numerous and long-looped flagella only in the presence of UFA at 5°C but not at 37°C. As flagella are well known to be involved in biofilm formation, this new finding raises questions about the structure and persistence of biofilms settled in refrigerated environments using unsaturated lipid-rich products.
Biocontrol of citrus fungal pathogens by lipopeptides produced by Bacillus velezensis TZ01
Authors: An Baoju , Du Danchao , Huang Zhendong , Pu Zhanxu , Lv Jia , Zhu Li , Liu Shunmin , Zhang Liping , Chen Guoqing , Lu Lianming
Published: September 2024

Citrus diseases caused by fungal pathogens drastically decreased the yield and quality of citrus fruits, leading to huge economic losses. Given the threats of chemical pesticides on the environment and human health, biocontrol agents have received considerable attention worldwide as ecofriendly and sustainable alternative to chemical fungicides. In the present study, we isolated a Bacillus velezensis strain TZ01 with potent antagonistic effect against three citrus pathogenic fungi: Diaporthe citri, Colletotrichum gloeosporioides and Alternaria alternata. The culture supernatant of this strain exhibited remarkable antifungal activity on potato dextrose agar plates and detached leaves of five citrus varieties. Treatment with TZ01 culture supernatant obviously affected the hyphal morphology and caused nucleic acid leakage. The crude lipopeptides (LPs) extracted from the culture supernatant were found as the major active ingredients, and could maintain the activity under a wide range of temperature and pH and ultraviolet radiation. Furthermore, the type of LPs, produced in vitro, were explored. Whole-genome sequencing of TZ01 revealed secondary metabolite gene clusters encoding synthetases for non-ribosomal peptides and polyketide production, and gene clusters responsible for the synthesis of three important LPs (surfactin, iturin, and fengycin) were identified in the genome. The liquid chromatography-mass spectrometry analysis confirmed the presence of various homologs of surfactin A, bacillomycin D, and fengycin A in the extracted LPs. Taken together, these results contribute to the possible biocontrol mechanisms of B. velezensis strain TZ01, as well as providing a promising new candidate strain as a biological control agent for controlling citrus fungal pathogens.
Tongue exercise ameliorates structural and functional upper airway deficits in a rodent model of hypoglossal motor neuron loss
Authors: Keilholz Amy N. , Pathak Ishan , Smith Catherine L. , Osman Kate L. , Smith Lauren , Oti Grace , Golzy Mojgan , Ma Lixin , Lever Teresa E. , Nichols Nicole L.
Published: September 2024

Introduction

Tongue weakness and atrophy can lead to deficits in the vital functions of breathing and swallowing in patients with motor neuron diseases (MNDs; e.g., amyotrophic lateral sclerosis (ALS) and pseudobulbar palsy), often resulting in aspiration pneumonia, respiratory failure, and death. Available treatments for patients with MNDs are largely palliative; thus, there is a critical need for therapies targeting preservation of upper airway function and suggesting a role for tongue exercise in patients with MNDs. Here, we leveraged our inducible rodent model of hypoglossal (XII) motor neuron degeneration to investigate the effects of a strength endurance tongue exercise program on upper airway structure and function. Our model was created through intralingual injection of cholera toxin B conjugated to saporin (CTB-SAP) into the genioglossus muscle of the tongue to induce targeted death of XII motor neurons.

Methods

Rats in this study were allocated to 4 experimental groups that received intralingual injection of either CTB-SAP or unconjugated CTB + SAP (i.e., control) +/- tongue exercise. Following tongue exercise exposure, we evaluated the effect on respiratory function (via plethysmography), macrostructure [via magnetic resonance imaging (MRI) of the upper airway and tongue], and ultrafine structure [via ex vivo magnetic resonance spectroscopy (MRS) of the tongue] with a focus on lipid profiles.

Results

Results showed that sham exercise-treated CTB-SAP rats have evidence of upper airway restriction (i.e., reduced airflow) and structural changes present in the upper airway (i.e., airway compression) when compared to CTB-SAP + exercise rats and control rats +/- tongue exercise, which was ameliorated with tongue exercise. Additionally, CTB-SAP + sham exercise rats have evidence of increased lipid expression in the tongue consistent with previously observed tongue hypertrophy when compared to CTB-SAP + exercise rats or control rats +/- tongue exercise.

Conclusion

These findings provide further evidence that a strength endurance tongue exercise program may be a viable therapeutic treatment option in patients with XII motor neuron degeneration in MNDs such as ALS. Future directions will focus on investigating the underlying mechanism responsible for tongue exercise-induced plasticity in the hypoglossal-tongue axis, particularly inflammatory associated factors such as BDNF.
Associations between concussion and more severe TBIs, mild cognitive impairment, and early-onset dementia among military retirees over 40 years
Authors: Belding Jennifer N. , Bonkowski James , Englert Robyn , Grimes Stanfill Ansley , Tsao Jack W.
Published: September 2024

Background and objectives

As the population of U.S. service members (SMs) who have sustained concussions and more severe traumatic brain injuries (TBIs) during military service ages, understanding the long-term outcomes associated with such injuries will provide critical information that may promote long-term assessment, support, and rehabilitation following military service. The objective of this research was to examine whether concussion and more severe TBIs are associated with greater risk of precursors to dementia (i.e., mild cognitive impairment, memory loss), early-onset dementia, and any dementia.

Methods

This study used a retrospective cohort design wherein archival medical and career records from 1980 to 2020 identified U.S. military personnel who retired from military service and their corresponding Tricare-reimbursable medical encounters in inpatient and/or outpatient settings in military treatment facilities and/or purchased care settings both before and after retirement. All military personnel who served on active duty between 1980 and 2020 and were at least 45 years of age by 2020 were eligible for inclusion (N = 6,092,432). Those who were discharged from military service with a retirement designation, and were thus eligible for Tricare for Life, were included in the analytic sample (N = 1,211,972). Diagnoses of concussion and more severe TBI during active duty service recorded in inpatient settings between 1980 and 2020 and in outpatient settings from 2001 to 2020 were identified. Focal outcomes of interest included memory loss, mild cognitive impairment, Alzheimer’s, Lewy Body dementia, frontotemporal dementia, and vascular dementia. Dementia diagnoses before age 65 were labeled early-onset.

Results

Those with (vs. without) concussion diagnoses during military service were significantly more likely to be diagnosed with memory loss and mild cognitive impairment and any of the dementias examined. However, they were not at greater risk of being diagnosed with early-onset dementia.

Discussion

Military SMs diagnosed with concussion may be at elevated risk for long-term neurodegenerative outcomes including memory loss, mild cognitive impairment, and dementia. As the population of SMs who sustained TBI during the Global War on Terror continue to age, the prevalence of dementia will increase and may bring a unique burden to the VHA.
Is non-invasive neuromodulation a viable technique to improve neuroplasticity in individuals with acquired brain injury? A review
Authors: Eliason Michelle , Kalbande Prajakta Premchand , Saleem Ghazala T.
Published: September 2024

Objective

This study aimed to explore and evaluate the efficacy of non-invasive brain stimulation (NIBS) as a standalone or coupled intervention and understand its mechanisms to produce positive alterations in neuroplasticity and behavioral outcomes after acquired brain injury (ABI).

Data sources

Cochrane Library, Web of Science, PubMed, and Google Scholar databases were searched from January 2013 to January 2024.

Study selection

Using the PICO framework, transcranial magnetic stimulation (TMS) and transcranial direct current stimulation (tDCS) randomized controlled trials (RCTs), retrospective, pilot, open-label, and observational large group and single-participant case studies were included. Two authors reviewed articles according to pre-established inclusion criteria.

Data extraction

Data related to participant and intervention characteristics, mechanisms of change, methods, and outcomes were extracted by two authors. The two authors performed quality assessments using SORT.

Results

Twenty-two studies involving 657 participants diagnosed with ABIs were included. Two studies reported that NIBS was ineffective in producing positive alterations or behavioral outcomes. Twenty studies reported at least one, or a combination of, positively altered neuroplasticity and improved neuropsychological, neuropsychiatric, motor, or somatic symptoms. Twenty-eight current articles between 2020 and 2024 have been studied to elucidate potential mechanisms of change related to NIBS and other mediating or confounding variables.

Discussion

tDCS and TMS may be efficacious as standalone interventions or coupled with neurorehabilitation therapies to positively alter maladaptive brain physiology and improve behavioral symptomology resulting from ABI. Based on postintervention and follow-up results, evidence suggests NIBS may offer a direct or mediatory contribution to improving behavioral outcomes post-ABI.

Conclusion

More research is needed to better understand the extent of rTMS and tDCS application in affecting changes in symptoms after ABI.
Case report: Novel use of clinical brain-computer interfaces in recreation programming for an autistic adolescent with co-occurring attention deficit hyperactivity disorder
Authors: Van Damme Susannah , Mumford Leslie , Johnson Aleah , Chau Tom
Published: September 2024

Background

In recent years, several autistic1 children and youth have shown interest in Holland Bloorview Kids Rehabilitation Hospital’s clinical brain computer interface (BCI) program. Existing literature about BCI use among autistic individuals has focused solely on cognitive skill development and remediation of challenging behaviors. To date, the benefits of recreational BCI programming with autistic children and youth have not been documented.

Purpose

This case report summarizes the experiences of an autistic male adolescent with co-occurring attention deficit hyperactivity disorder using a BCI for recreation and considers possible benefits with this novel user population.

Methods

A single retrospective chart review was completed with parental guardian’s consent.

Findings

The participant demonstrated enjoyment in BCI sessions and requested continued opportunities to engage in BCI programming. This enjoyment correlated with improved Canadian Occupational Performance Measure (COPM) scores in BCI programming, outperforming scores from other recreational programs. Additionally, clinicians observed changes in social communication efforts and self-advocacy in this first autistic participant.

Conclusion

The use of brain computer interfaces in recreational programming provides a novel opportunity for engagement for autistic children and youth that may also support skill development.
Super-resolution reconstruction improves multishell diffusion: using radiomics to predict adult-type diffuse glioma IDH and grade
Authors: Zhang Chi , Wang Peng , He Jinlong , Wu Qiong , Xie Shenghui , Li Bo , Hao Xiangcheng , Wang Shaoyu , Zhang Huapeng , Hao Zhiyue , Gao Weilin , Liu Yanhao , Guo Jiahui , Hu Mingxue , Gao Yang
Published: September 2024

Objectives

Multishell diffusion scanning is limited by low spatial resolution. We sought to improve the resolution of multishell diffusion images through deep learning-based super-resolution reconstruction (SR) and subsequently develop and validate a prediction model for adult-type diffuse glioma, isocitrate dehydrogenase status and grade 2/3 tumors.

Materials and methods

A simple diffusion model (DTI) and three advanced diffusion models (DKI, MAP, and NODDI) were constructed based on multishell diffusion scanning. Migration was performed with a generative adversarial network based on deep residual channel attention networks, after which images with 2x and 4x resolution improvements were generated. Radiomic features were used as inputs, and diagnostic models were subsequently constructed via multiple pipelines.

Results

This prospective study included 90 instances (median age, 54.5 years; 39 men) diagnosed with adult-type diffuse glioma. Images with both 2x- and 4x-improved resolution were visually superior to the original images, and the 2x-improved images allowed better predictions than did the 4x-improved images (P<.001). A comparison of the areas under the curve among the multiple pipeline-constructed models revealed that the advanced diffusion models did not have greater diagnostic performance than the simple diffusion model (P>.05). The NODDI model constructed with 2x-improved images had the best performance in predicting isocitrate dehydrogenase status (AUC_validation=0.877; Brier score=0.132). The MAP model constructed with the original images performed best in classifying grade 2 and grade 3 tumors (AUC_validation=0.806; Brier score=0.168).

Conclusion

SR improves the resolution of multishell diffusion images and has different advantages in achieving different goals and creating different target diffusion models.
ColocZStats: a z-stack signal colocalization extension tool for 3D slicer
Authors: Chen Xiang , Thakur Teena , Jeyasekharan Anand D. , Benoukraf Touati , Meruvia-Pastor Oscar
Published: September 2024

Confocal microscopy has evolved to be a widely adopted imaging technique in molecular biology and is frequently utilized to achieve accurate subcellular localization of proteins. Applying colocalization analysis on image z-stacks obtained from confocal fluorescence microscopes is a dependable method of revealing the relationship between different molecules. In addition, despite the established advantages and growing adoption of 3D visualization software in various microscopy research domains, there have been few systems that can support colocalization analysis within a user-specified region of interest (ROI). In this context, several broadly employed biological image visualization platforms are meticulously explored in this study to understand the current landscape. It has been observed that while these applications can generate three-dimensional (3D) reconstructions for z-stacks, and in some cases transfer them into an immersive virtual reality (VR) scene, there is still little support for performing quantitative colocalization analysis on such images based on a user-defined ROI and thresholding levels. To address these issues, an extension called ColocZStats (pronounced Coloc-Zee-Stats) has been developed for 3D Slicer, a widely used free and open-source software package for image analysis and scientific visualization. With a custom-designed user-friendly interface, ColocZStats allows investigators to conduct intensity thresholding and ROI selection on imported 3D image stacks. It can deliver several essential colocalization metrics for structures of interest and produce reports in the form of diagrams and spreadsheets.