bluuebunny/crossref_metadata_2025 huggingface dataset:
Dimethyl Fumarate Induces Apoptosis via Inhibition of NF-κB and Enhances the Effect of Paclitaxel and Adriamycin in Human TNBC Cells
Authors: Tsurushima Katsumasa , Tsubaki Masanobu , Takeda Tomoya , Matsuda Takuya , Kimura Akihiro , Takefuji Honoka , Okada Akane , Sakamoto Chiaki , Ishizaka Toshihiko , Nishida Shozo
Published: August 2022

Triple-negative breast cancer (TNBC) has the poorest prognosis of all breast cancer subtypes. Recently, the activation of NF-κB, which is involved in the growth and survival of malignant tumors, has been demonstrated in TNBC, suggesting that NF-κB may serve as a new therapeutic target. In the present study, we examined whether dimethyl fumarate (DMF), an NF-κB inhibitor, induces apoptosis in TNBC cells and enhances the apoptosis-inducing effect of paclitaxel and adriamycin. Cell survival was analyzed by the trypan blue assay and apoptosis assay. Protein detection was examined by immunoblotting. The activation of NF-κB p65 was correlated with poor prognosis in patients with TNBC. DMF induced apoptosis in MDA-MB-231 and BT-549 cells at concentrations that were non-cytotoxic to the normal mammary cell line MCF-10A. Furthermore, DMF inhibited NF-κB nuclear translocation and Survivin, XIAP, Bcl-xL, and Bcl-2 expression in MDA-MB-231 and BT-549 cells. Moreover, DMF enhanced the apoptosis-inducing effect of paclitaxel and adriamycin in MDA-MB-231 cells. These findings suggest that DMF may be an effective therapeutic agent for the treatment of TNBC, in which NF-κB is constitutively active. DMF may also be useful as an adjuvant therapy to conventional anticancer drugs.
Phloroglucinol Derivative Carbomer Hydrogel Accelerates MRSA-Infected Wounds’ Healing
Authors: Huang Xiaosu , Yang Junhua , Zhang Renyue , Ye Lianbao , Li Ming , Chen Weiqiang
Published: August 2022

Globally, wound infection is considered to be one of the major healthcare problems, with bacterial infections being the most critical threat, leading to poor and delayed wound healing, and even death. As a superbug, methicillin-resistant Staphylococcus aureus (MRSA) causes a profound hazard to public health safety, prompting us to search for alternative treatment approaches. Herein, the MTT test and Hoechst/propidium iodide (PI) staining demonstrated that PD was slightly less toxic to human fibroblasts including Human keratinocytes (HaCaT) cell line than Silver sulfadiazine (SSD), and Vancomycin (Van). In the MRSA-infected wound model, PD hydrogel (1%, 2.5%) was applied with for 14 days. The wound healing of PD hydrogel groups was superior to the SSD, Van, and control groups. Remarkably, the experimental results showed that PD reduced the number of skin bacteria, reduced inflammation, and upregulated the expression of PCNA (keratinocyte proliferation marker) and CD31 (angiogenesis manufacturer) at the wound site by histology (including hematoxylin–eosin (HE) staining, Masson staining) and immunohistochemistry. Additionally, no toxicity, hemocompatibility or histopathological changes to organs were observed. Altogether, these results suggested the potential of PD hydrogel as a safe, effective, and low toxicity hydrogel for the future clinical treatment of MRSA-infected wounds.
Bidirectional Control between Cholesterol Shuttle and Purine Signal at the Central Nervous System
Authors: Passarella Daniela , Ronci Maurizio , Di Liberto Valentina , Zuccarini Mariachiara , Mudò Giuseppa , Porcile Carola , Frinchi Monica , Di Iorio Patrizia , Ulrich Henning , Russo Claudio
Published: August 2022

Recent studies have highlighted the mechanisms controlling the formation of cerebral cholesterol, which is synthesized in situ primarily by astrocytes, where it is loaded onto apolipoproteins and delivered to neurons and oligodendrocytes through interactions with specific lipoprotein receptors. The “cholesterol shuttle” is influenced by numerous proteins or carbohydrates, which mainly modulate the lipoprotein receptor activity, function and signaling. These molecules, provided with enzymatic/proteolytic activity leading to the formation of peptide fragments of different sizes and specific sequences, could be also responsible for machinery malfunctions, which are associated with neurological, neurodegenerative and neurodevelopmental disorders. In this context, we have pointed out that purines, ancestral molecules acting as signal molecules and neuromodulators at the central nervous system, can influence the homeostatic machinery of the cerebral cholesterol turnover and vice versa. Evidence gathered so far indicates that purine receptors, mainly the subtypes P2Y2, P2X7 and A2A, are involved in the pathogenesis of neurodegenerative diseases, such as Alzheimer’s and Niemann–Pick C diseases, by controlling the brain cholesterol homeostasis; in addition, alterations in cholesterol turnover can hinder the purine receptor function. Although the precise mechanisms of these interactions are currently poorly understood, the results here collected on cholesterol–purine reciprocal control could hopefully promote further research.
Vitamin D-Related Single Nucleotide Polymorphisms as Risk Biomarker of Cardiovascular Disease
Authors: González Rojo Paula , Pérez Ramírez Cristina , Gálvez Navas José María , Pineda Lancheros Laura Elena , Rojo Tolosa Susana , Ramírez Tortosa María del Carmen , Jiménez Morales Alberto
Published: August 2022

Cardiovascular diseases (CVDs) are a group of disorders of the heart and blood vessels. In addition to environmental risk factors, genetic predisposition increases the risk; this includes alterations in the vitamin D receptor gene (VDR). These alterations play a key role in modifying vitamin D uptake, being able to modify its function and increasing susceptibility to cardiovascular disorders. The aim of this study was to evaluate the association of polymorphisms in the VDR gene and risk of CVD in a Caucasian population. A retrospective case-control study was conducted comprising 246 CVD patients and 246 controls of Caucasian origin from Southern Spain. The genetic polymorphisms BsmI (rs1544410), TaqI (rs731236), ApaI (rs7975232), FokI (rs2228570) and Cdx2 (rs11568820) were determined by means of real-time polymerase chain reaction (PCR) for allelic discrimination using TaqMan® probes. The logistic regression analysis adjusted for body mass index and diabetes revealed that the TT genotype was associated with a higher risk of CVD in both the genotypic model (p = 0.0430; OR = 2.30; 95% CI = 1.06–5.37; TT vs. CC) and the recessive model (p = 0.0099; OR = 2.71; 95% CI = 1.31–6.07; TT vs. C). Haplotype analysis revealed that the haplotype GAC (p = 0.047; OR = 0.34; 95% CI = 0.12–0.98) was associated with increased risk of CVD. The VDR polymorphisms FokI (rs2228570) was significantly associated with the development of CVD. No influence was observed of the VDR polymorphisms BsmI (rs1544410), TaqI (rs731236), ApaI (rs7975232) and Cdx2 (rs11568820) on the risk of developing CVD in the patients studied.
Roles of Focal Adhesion Kinase PTK2 and Integrin αIIbβ3 Signaling in Collagen- and GPVI-Dependent Thrombus Formation under Shear
Authors: Huang Jingnan , Jooss Natalie J. , Fernández Delia I. , Sickmann Albert , García Ángel , Wichapong Kanin , Dijkgraaf Ingrid , Heemskerk Johan W. M.
Published: August 2022

Glycoprotein (GP)VI and integrin αIIbβ3 are key signaling receptors in collagen-dependent platelet aggregation and in arterial thrombus formation under shear. The multiple downstream signaling pathways are still poorly understood. Here, we focused on disclosing the integrin-dependent roles of focal adhesion kinase (protein tyrosine kinase 2, PTK2), the shear-dependent collagen receptor GPR56 (ADGRG1 gene), and calcium and integrin-binding protein 1 (CIB1). We designed and synthetized peptides that interfered with integrin αIIb binding (pCIB and pCIBm) or mimicked the activation of GPR56 (pGRP). The results show that the combination of pGRP with PTK2 inhibition or of pGRP with pCIB > pCIBm in additive ways suppressed collagen- and GPVI-dependent platelet activation, thrombus buildup, and contraction. Microscopic thrombus formation was assessed by eight parameters (with script descriptions enclosed). The suppressive rather than activating effects of pGRP were confined to blood flow at a high shear rate. Blockage of PTK2 or interference of CIB1 no more than slightly affected thrombus formation at a low shear rate. Peptides did not influence GPVI-induced aggregation and Ca2+ signaling in the absence of shear. Together, these data reveal a shear-dependent signaling axis of PTK2, integrin αIIbβ3, and CIB1 in collagen- and GPVI-dependent thrombus formation, which is modulated by GPR56 and exclusively at high shear. This work thereby supports the role of PTK2 in integrin αIIbβ3 activation and signaling.
Crosstalk between Growth and Osmoregulation of GHRH-SST-GH-IGF Axis in Triploid Rainbow Trout (Oncorhynchus mykiss)
Authors: Xiang Kaiwen , Yang Qian , Liu Mengqun , Yang Xiaodong , Li Jifang , Hou Zhishuai , Wen Haishen
Published: August 2022

Smolting is an important development stage of salmonid, and an energy trade-off occurs between osmotic regulation and growth during smolting in rainbow trout (Oncorhynchus mykiss). Growth hormone releasing hormone, somatostatin, growth hormone and insulin-like growth factor (GHRH-SST-GH-IGF) axis exhibit pleiotropic effects in regulating growth and osmotic adaptation. Due to salmonid specific genome duplication, increased paralogs are identified in the ghrh-sst-gh-igf axis, however, their physiology in modulating osmoregulation has yet to be investigated. In this study, seven sst genes (sst1a, sst1b, sst2, sst3a, sst3b, sst5, sst6) were identified in trout. We further investigated the ghrh-sst-gh-igf axis of diploid and triploid trout in response to seawater challenge. Kidney sst (sst1b, sst2, sst5) and sstr (sstr1b1, sstr5a, sstr5b) expressions were changed (more than 2-fold increase (except for sstr5a with 1.99-fold increase) or less than 0.5-fold decrease) due to osmoregulation, suggesting a pleiotropic physiology of SSTs in modulating growth and smoltification. Triploid trout showed significantly down-regulated brain sstr1b1 and igfbp2a1 (p < 0.05), while diploid trout showed up-regulated brain igfbp1a1 (~2.61-fold, p = 0.057) and igfbp2a subtypes (~1.38-fold, p < 0.05), suggesting triploid trout exhibited a better acclimation to the seawater environment. The triploid trout showed up-regulated kidney igfbp5a subtypes (~6.62 and 7.25-fold, p = 0.099 and 0.078) and significantly down-regulated igfbp5b2 (~0.37-fold, p < 0.05), showing a conserved physiology of teleost IGFBP5a in regulating osmoregulation. The IGFBP6 subtypes are involved in energy and nutritional regulation. Distinctive igfbp6 subtypes patterns (p < 0.05) potentially indicated trout triggered energy redistribution in brain and kidney during osmoregulatory regulation. In conclusion, we showed that the GHRH-SST-GH-IGF axis exhibited pleiotropic effects in regulating growth and osmoregulatory regulation during trout smolting, which might provide new insights into seawater aquaculture of salmonid species.
Bioinformatics Analysis of the Periodicity in Proteins with Coiled-Coil Structure—Enumerating All Decompositions of Sequence Periods
Authors: Then Andre , Zhang Haotian , Ibrahim Bashar , Schuster Stefan
Published: August 2022

A coiled coil is a structural motif in proteins that consists of at least two α-helices wound around each other. For structural stabilization, these α-helices form interhelical contacts via their amino acid side chains. However, there are restrictions as to the distances along the amino acid sequence at which those contacts occur. As the spatial period of the α-helix is 3.6, the most frequent distances between hydrophobic contacts are 3, 4, and 7. Up to now, the multitude of possible decompositions of α-helices participating in coiled coils at these distances has not been explored systematically. Here, we present an algorithm that computes all non-redundant decompositions of sequence periods of hydrophobic amino acids into distances of 3, 4, and 7. Further, we examine which decompositions can be found in nature by analyzing the available data and taking a closer look at correlations between the properties of the coiled coil and its decomposition. We find that the availability of decompositions allowing for coiled-coil formation without putting too much strain on the α-helix geometry follows an oscillatory pattern in respect of period length. Our algorithm supplies the basis for exploring the possible decompositions of coiled coils of any period length.
Effective Optical Image Assessment of Cellulose Paper Immunostrips for Blood Typing
Authors: Ratajczak Katarzyna , Sklodowska-Jaros Karolina , Kalwarczyk Ewelina , Michalski Jacek A. , Jakiela Slawomir , Stobiecka Magdalena
Published: August 2022

Novel high-performance biosensing devices, based on a microporous cellulose matrix, have been of great interest due to their high sensitivity, low cost, and simple operation. Herein, we report on the design and testing of portable paper-based immunostrips (IMS) for in-field blood typing in emergencies requiring blood transfusion. Cellulose fibrils of a paper membrane were functionalized with antibodies via supramolecular interactions. The formation of hydrogen bonds between IgM pentamer and cellulose fibers was corroborated using quantum mechanical calculations with a model cellulose chain and a representative amino acid sequence. In the proposed immunostrips, paper with a pore size of 3 µm dia. was used to enable functionalization of its channels with antibody molecules while blocking the red blood cells (RBC) from channel entering. Under the optimized test conditions, all blood types of AB0 and Rh system could be determined by naked eye examination, requiring only a small blood sample (3.5 µL). The durability of IgM immunostrips against storing has been tested. A new method of statistical evaluation of digitized blood agglutination images, compatible with a clinical five-level system, has been proposed. Critical parameters of the agglutination process have been established to enable future development of automatic blood typing with machine vision and digital data processing.
Gene Variants Related to Cardiovascular and Pulmonary Diseases May Correlate with Severe Outcome of COVID-19
Authors: Sypniewski Mateusz , Król Zbigniew J. , Szyda Joanna , Kaja Elżbieta , Mroczek Magdalena , Suchocki Tomasz , Lejman Adrian , Stępień Maria , Topolski Piotr , Dąbrowski Maciej , Kotlarz Krzysztof , Aplas Angelika , Wasiak Michał , Wojtaszewska Marzena , Zawadzki Paweł , Pawlak Agnieszka , Gil Robert , Dobosz Paula , Stojak Joanna
Published: August 2022

Background: Severe outcomes of COVID-19 account for up to 15% of all cases. The study aims to check if any gene variants related to cardiovascular (CVD) and pulmonary diseases (PD) are correlated with a severe outcome of COVID-19 in a Polish cohort of COVID-19 patients. Methods: In this study, a subset of 747 samples from unrelated individuals collected across Poland in 2020 and 2021 was used and whole-genome sequencing was performed. Results: The GWAS analysis of SNPs and short indels located in genes related to CVD identified one variant significant in COVID-19 severe outcome in the HADHA gene, while for the PD gene panel, we found two significant variants in the DRC1 gene. In this study, both potentially protective and risk variants were identified, of which variants in the HADHA gene deserve the most attention. Conclusions: This is the first study reporting the association between the HADHA and DRC1 genetic variants and COVID-19 severe outcome based on the cohort WGS analysis. Although all the identified variants are localised in introns, they may be correlated and therefore inherited along with other risk variants, potentially causative to severe outcome of COVID-19 but not discovered yet.
A Pilot Study on Peptidylarginine Deiminases and Protein Deimination in Animal Cancers across Vertebrate Species
Authors: Inal Jameel M. , Hristova Mariya , Lange Sigrun
Published: August 2022

PADs are a group of calcium-dependent enzymes that play key roles in inflammatory pathologies and have diverse roles in cancers. PADs cause irreversible post-translational modification of arginine to citrulline, leading to changes in protein function in different cellular compartments. PAD isozyme diversity differs throughout phylogeny in chordates, with five PAD isozymes in mammals, three in birds, and one in fish. While the roles for PADs in various human cancers are mounting (both in regards to cancer progression and epigenetic regulation), investigations into animal cancers are scarce. The current pilot-study therefore aimed at assessing PAD isozymes in a range of animal cancers across the phylogeny tree. In addition, the tissue samples were assessed for total protein deimination and histone H3 deimination (CitH3), which is strongly associated with human cancers and also indicative of gene regulatory changes and neutrophil extracellular trap formation (NETosis). Cancers were selected from a range of vertebrate species: horse, cow, reindeer, sheep, pig, dog, cat, rabbit, mink, hamster, parrot, and duck. The cancers chosen included lymphoma, kidney, lung, testicular, neuroendocrine, anaplastic, papilloma, and granulosa cell tumour. Immunohistochemical analysis revealed that CitH3 was strongly detected in all of the cancers assessed, while pan-deimination detection was overall low. Both PAD2 and PAD3 were the most predominantly expressed PADs across all of the cancers assessed, while PAD1, PAD4, and PAD6 were overall expressed at lower, albeit varying, levels. The findings from this pilot study provide novel insights into PAD-mediated roles in different cancers across a range of vertebrate species and may aid in the understanding of cancer heterogeneity and cancer evolution.
c-Kit Induces Migration of Triple-Negative Breast Cancer Cells and Is a Promising Target for Tyrosine Kinase Inhibitor Treatment
Authors: López-Mejía José A. , Tallabs-Utrilla Luis F. , Salazar-Sojo Pablo , Mantilla-Ollarves Jessica C. , Sánchez-Carballido Manuel A. , Rocha-Zavaleta Leticia
Published: August 2022

Triple-negative breast cancer (TNBC) is associated with a poor prognosis and the absence of targeted therapy. c-Kit, a receptor tyrosine kinase (RTK), is considered a molecular target for anticancer drugs. Tyrosine kinase inhibitors (TKIs) recognizing c-Kit are used for the treatment of c-Kit-expressing tumors. However, the expression, function, and therapeutic potential of c-Kit have been little explored in TNBC. Here, we studied the expression and effects of c-Kit in TNBC through in vitro and in silico analysis, and evaluated the response to TKIs targeting c-Kit. Analysis of TNBC cells showed the expression of functional c-Kit at the cell membrane. The stimulation of c-Kit with its ligand induced the activation of STAT3, Akt, and ERK1/2, increasing cell migration, but had no effect on cell proliferation or response to Doxorubicin. Analysis of public datasets showed that the expression of c-Kit in tumors was not associated with patient survival. Finally, TNBC cells were susceptible to TKIs, in particular the effect of Nilotinib was stronger than Doxorubicin in all cell lines. In conclusion, TNBC cells express functional c-Kit, which is a targetable molecule, and show a strong response to Nilotinib that may be considered a candidate drug for the treatment of TNBC.
Four Cholesterol-Recognition Motifs in the Pore-Forming and Translocation Domains of Adenylate Cyclase Toxin Are Essential for Invasion of Eukaryotic Cells and Lysis of Erythrocytes
Authors: Amuategi Jone , Alonso Rocío , Ostolaza Helena
Published: August 2022

Adenylate Cyclase Toxin (ACT or CyaA) is one of the important virulence factors secreted by Bordetella pertussis, the bacterium causative of whooping cough. ACT debilitates host defenses by production of unregulated levels of cAMP into the cell cytosol upon delivery of its N-terminal domain with adenylate cyclase activity (AC domain) and by forming pores in the plasma membrane of macrophages. Binding of soluble toxin monomers to the plasma membrane of target cells and conversion into membrane-integrated proteins are the first and last step for these toxin activities; however, the molecular determinants in the protein or the target membrane that govern this conversion to an active toxin form are fully unknown. It was previously reported that cytotoxic and cytolytic activities of ACT depend on membrane cholesterol. Here we show that ACT specifically interacts with membrane cholesterol, and find in two membrane-interacting ACT domains, four cholesterol-binding motifs that are essential for AC domain translocation and lytic activities. We hypothesize that direct ACT interaction with membrane cholesterol through those four cholesterol-binding motifs drives insertion and stabilizes the transmembrane topology of several helical elements that ultimately build the ACT structure for AC delivery and pore-formation, thereby explaining the cholesterol-dependence of the ACT activities. The requirement for lipid-mediated stabilization of transmembrane helices appears to be a unifying mechanism to modulate toxicity in pore-forming toxins.
A New 360° Framework to Predict Customer Lifetime Value for Multi-Category E-Commerce Companies Using a Multi-Output Deep Neural Network and Explainable Artificial Intelligence
Authors: Yılmaz Benk Gülşah , Badur Bertan , Mardikyan Sona
Published: August 2022

Online purchasing has developed rapidly in recent years due to its efficiency, convenience, low cost, and product variety. This has increased the number of online multi-category e-commerce retailers that sell a variety of product categories. Due to the growth in the number of players, each company needs to optimize its own business strategy in order to compete. Customer lifetime value (CLV) is a common metric that multi-category e-commerce retailers usually consider for competition because it helps determine the most valuable customers for the retailers. However, in this paper, we introduce two additional novel factors in addition to CLV to determine which customers will bring in the highest revenue in the future: distinct product category (DPC) and trend in amount spent (TAS). Then, we propose a new framework. We utilized, for the first time in the relevant literature, a multi-output deep neural network (DNN) model to test our proposed framework while forecasting CLV, DPC, and TAS together. To make this outcome applicable in real life, we constructed customer clusters that allow the management of multi-category e-commerce companies to segment end-users based on the three variables. We compared the proposed framework (constructed with multiple outputs: CLV, DPC, and TAS) against a baseline single-output model to determine the combined effect of the multi-output model. In addition, we also compared the proposed model with multi-output Decision Tree (DT) and multi-output Random Forest (RF) algorithms on the same dataset. The results indicate that the multi-output DNN model outperforms the single-output DNN model, multi-output DT, and multi-output RF across all assessment measures, proving that the multi-output DNN model is more suitable for multi-category e-commerce retailers’ usage. Furthermore, Shapley values derived through the explainable artificial intelligence method are used to interpret the decisions of the DNN. This practice demonstrates which inputs contribute more to the outcomes (a significant novelty in interpreting the DNN model for the CLV).
Unexpected Previously Unknown Diversity of the Genus Microphor Macquart (Diptera: Dolichopodidae: Microphorinae) in the West Palaearctic
Authors: Barták Miroslav , Kubík Štěpán
Published: August 2022

Microphor baechlii sp. nov. (Switzerland, Turkey), M. chvalai sp. nov. (France), M. nevadensis sp. nov. (Spain), M. pallipes sp. nov. (Italy), and M. turcicus sp. nov. (Turkey) are described and illustrated. The neotype of M. anomalus (Meigen, 1824) is designated. Males of all known Palaearctic species of Microphor are keyed, genitalia are illustrated for new species and species previously inadequately illustrated, and main diagnostic characters are discussed. Microphorstrobli Chvála, 1986 is newly recorded from Bulgaria, M. anomalus (Meigen, 1824) is newly recorded from Turkey, and M. holosericeus (Meigen, 1804) is newly recorded from Turkey and Portugal.
Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)
Authors: Pan Xue , Pei Yanfang , Zhang Cuici , Huang Yaling , Chen Ling , Wei Liqiong , Li Chuanren , Dong Xiaolin , Chen Xiang
Published: August 2022

Insulin signaling can regulate various physiological functions, such as energy metabolism and reproduction and so on, in many insects, including mosquito and locust. However, the molecular mechanism of this physiological process remains elusive. The tobacco cutworm, Spodoptera litura, is one of the most important pests of agricultural crops around the world. In this study, phosphoinositide 3-kinase (SlPI3K), protein kinase B (SlAKT), target of rapamycin (SlTOR), ribosomal protein S6 kinase (SlS6K) and transcription factor cAMP-response element binding protein (SlCREB) genes, except transcription factor forkhead box class O (SlFoxO), can be activated by bovine insulin injection. Then, we studied the influence of the insulin receptor gene (SlInR) on the reproduction of S. litura using RNA interference technology. qRT-PCR analysis revealed that SlInR was most abundant in the head. The SlPI3K, SlAKT, SlTOR, SlS6K and SlCREB genes were decreased, except SlFoxO, after the SlInR gene knockdown. Further studies revealed that the expression of vitellogenin mRNA and protein, Methoprene-tolerant gene (SlMet), could be down-regulated by the injection of dsRNA of SlInR significantly. Furthermore, a depletion in the insulin receptor by RNAi significantly decreased the content of juvenile hormone III (JH-III), total proteins and triacylgycerol. These changes indicated that a lack of SlInR could impair ovarian development and decrease fecundity in S. litura. Our studies contribute to a comprehensive insight into reproduction, regulated by insulin and the juvenile hormone signaling pathway through nutrition, and a provide theoretical basis for the reproduction process in pest insects.
Predation Capacity of the Banded Thrips Aeolothrips intermedius for the Biological Control of the Onion Thrips Thrips tabaci
Authors: Abenaim Linda , Bedini Stefano , Greco Alessio , Giannotti Paolo , Conti Barbara
Published: August 2022

The onion thrips, Thrips tabaci, is a main insect pest for many field crops worldwide, with a particular preference for the species of the genus Allium. Aeolothrips intermedius is a banded thrips, whose larvae are considered the primary native predator of T. tabaci. Due of their predatory behaviour, A. intermedius larvae are considered a good candidate for biological control against thrips pests. However, limited information is available on the specific predation rate of A. intermedius against T. tabaci. The aim of our study was to evaluate the predatory efficiency of A. intermedius larvae against T. tabaci adults. Predation assays, performed under laboratory conditions, indicated that A. intermedius larvae begin to prey after an average of about 23 min, and the time taken by an A. intermedius larva to capture and subdue the prey until its death is about 26 min. Furthermore, the maximum number of prey that the A. intermedius larvae are able to kill in 12 h is up to eight adults of T. tabaci/A. intermedius larva.
Atmospheric and Geodesic Controls of Muon Rates: A Numerical Study for Muography Applications
Authors: Cohu Amélie , Tramontini Matias , Chevalier Antoine , Ianigro Jean-Christophe , Marteau Jacques
Published: August 2022

Muon tomography or muography is an innovative imaging technique using atmospheric muons. The technique is based on the detection of muons that have crossed a target and the measurement of their attenuation or deviation induced by the medium. Muon flux models are key ingredients to convert tomographic and calibration data into the 2D or 3D density maps of the target. Ideally, they should take into account all possible types of local effects, from geomagnetism to atmospheric conditions. Two approaches are commonly used: semi-empirical models or Monte Carlo simulations. The latter offers the advantage to tackle down many environmental and experimental parameters and also allows the optimization of the nearly horizontal muons flux, which remains a long-standing problem for many muography applications. The goal of this paper is to identify through a detailed simulation what kind of environmental and experimental effects may affect the muography imaging sensitivity and its monitoring performance. The results have been obtained within the CORSIKA simulation framework, which offers the possibility to tune various parameters. The paper presents the simulation’s configuration and the results obtained for the muon fluxes computed in various conditions.
Computer Flow Simulation and Verification for Turbine Blade Channel Formed by the C-90-22 A Profile
Authors: Osipov Sergey , Shcherbatov Ivan , Vegera Andrey , Bryzgunov Pavel , Makhmutov Bulat
Published: August 2022

Currently, software products for numerical simulation of fluid dynamics processes (Ansys, Star CCM+, Comsol) are widely used in the power engineering industry when designing new equipment. However, computer simulation methods embedded in proprietary software products make specialists choose grid settings, boundary conditions, and a solver providing the minimal deviation from experimental data with the maximal calculation speed. This paper analyzes the influence of the main grid settings and boundary conditions in the Ansys software package on the error in the computer simulation of flows in standard elements of power equipment and gives recommendations for their optimal choice. As standard elements were considered blade turbine channels formed by C-90-22 A profiles.
A Review on Analysis of Reinforced Recycled Rubber Composites
Authors: Kabakçi Gamze Cakir , Aslan Ozgur , Bayraktar Emin
Published: August 2022

Rubber recycling attracts considerable attention by a variety of industries around the world due to shrinking resources, increasing cost of raw materials, growing awareness of sustainable development, and environmental issues. Recycled rubber is commonly used in aeronautic, automotive, and transportation industries. In this study, recycled rubber composites designed with different reinforcements in the literature are scrutinized by means of toughening mechanisms, mechanical and physical properties, as well as microstructural and fracture surface analysis. Microscale reinforcements (glass bubbles, alumina fiber, etc.) and nanoscale reinforcements (nanosilica, graphene nanoplatelets, etc.) utilized as reinforcements in rubber composites are thoroughly reviewed. The general mechanical properties reported by previous studies, such as tensile, compressive, and flexural strength, are investigated with the main goal of optimizing the amount of reinforcement used. The majority of the studies on recycled rubber composites show that recycled rubber reinforced with microscale particles leads to the development of physical and mechanical properties of the structures and also provides low-cost and lightweight composites for several application areas. Moreover, recycled rubber containing composites can be suitable for applications where high toughness and high resistance to impact are desirable. The present review aims to demonstrate research on reinforced recycled rubber composites in the literature and prospective outcomes.
Comparative Proteomics Study on the Postharvest Senescence of Volvariella volvacea
Authors: Zha Lei , Chen Mingjie , Guo Qian , Tong Zongjun , Li Zhengpeng , Yu Changxia , Yang Huanling , Zhao Yan
Published: August 2022

Volvariella volvacea is difficult to store after harvest, which restricts the production and circulation of V. volvacea fruiting bodies. Low-temperature storage is the traditional storage method used for most edible fungi. However, V. volvacea undergoes autolysis at low temperatures. When fruiting bodies are stored at 15 °C (suitable temperature), V. volvacea achieves the best fresh-keeping effect. However, the molecular mechanism underlying the postharvest senescence of V. volvacea remains unclear. Based on this information, we stored V. volvacea fruiting bodies at 15 °C after harvest and then analyzed the texture and phenotype combined with the results of previous physiological research. Four time points (0, 24, 60, and 96 h) were selected for the comparative proteomics study of V. volvacea during storage at 15 °C. A variety of proteins showed differential expressions in postharvest V. volvacea at 15 °C. Further comparison of the gene ontology (GO) enrichment analysis and KEGG pathways performed at different sampling points revealed proteins that were significantly enriched at several time points. At the same time, we also analyzed differentially expressed proteins (DEPs) related to the RNA transport, fatty acid biosynthesis and metabolism, and amino acid biosynthesis and metabolism pathways, and discussed the molecular functions of the PAB1, RPG1, ACC1, ADH3, ADH2, ALD5, and SDH2 proteins in postharvest V. volvacea senescence. Our results showed that many biological processes of the postharvest senescence of V. volvacea changed. Most importantly, we found that most RNA transport-related proteins were down-regulated, which may lead to a decrease in related gene regulation. Our results also showed that the expression of other important proteins, such as the fatty acid metabolism related proteins increased; and changes in fatty acid composition affected the cell membrane, which may accelerate the ripening and perception of V. volvacea fruiting bodies. Therefore, our research provides a reference for further studies on the aging mechanism of V. volvacea.
Special Issue “Sporothrix and Sporotrichosis 2.0”
Authors: Mora-Montes Héctor M.
Published: August 2022

Sporotrichosis is a chronic fungal disease of humans and other mammals that often affects the skin and subcutaneous tissues and, rarely, deep-seated organs (most frequently in immunocompetent hosts) [...]
Land-Use Transformation and Landscape Ecological Risk Assessment in the Three Gorges Reservoir Region Based on the “Production–Living–Ecological Space” Perspective
Authors: Liang Tian , Yang Fei , Huang Dan , Luo Yinchen , Wu You , Wen Chuanhao
Published: August 2022

Rapid urbanization and land-use change cause risk in regional ecological security. It is very significance to explore the evolutionary trend of land-use change and landscape ecological risk (LER) in an ecologically fragile area, especially in terms of maintaining sustainable development in a regional ecological environment. We selected the Three Gorges Reservoir Region (TGRR) as the study area based on land-use and land-cover data for 2000, 2010, and 2020. The land-use classification system used here was constructed using the perspective of the production–living–ecological space (PLES). The GIS spatial-analysis technique and FRAGSTATS 4 software were used. We used the method of the land-use transfer matrix, the landscape ecological risk assessment model, the ecological contribution rate of land-use transfer, and spatial autocorrelation analysis. We performed quantitative analysis of the spatio-temporal pattern of PLES and its LER in the TGRR over the past 20 years. The results show that: (1) The area of human living space (HLS) has expanded significantly—by 1469.37 km2 (+326.66%), while the area of agricultural production space (APS) has been compressed by both the urban/rural living space (URLS) and the forestland ecological space (FES), particularly during the last 10 years; (2) The overall LER results were medium, but LER is increasing; (3) The LER in the northern area of the Yangtze River is higher than in the south. The Wanzhou district and the downstream areas had a lower LER; and (4) The transformation from agricultural production space to forestland ecological space and urban/rural living space has had a higher contribution rate to the LER compared to other events. These results can be used as a reference for land planning, sustainable development, and ecological civilization construction in ecologically fragile areas.
Land System Reform in Rural China: Path and Mechanism
Authors: Shi Xinjie , Gao Xuwen , Fang Shile
Published: August 2022

Changes in land policy not only determine agricultural growth, but also have bearing on issues, such as peasants’ rights and interests, industrial development, and urbanization. On account of China’s singular history and culture, its land systems are somewhat unique. This paper analyzes the institutional changes that took place before and after 1978 to show the journey of land system reform in rural China in the 20th century. We look closely at the policy performance and drawbacks of the reform that occurred after 1978, and analyze a series of “Central First Documents” that concern rural issues. Based on this analysis, some prospective land policy reforms are predicated. We conclude that at present, rural land ownership is unlikely to be completely privatized in the short-term. Creating a new agricultural management system with special functions or a land shareholding system, could be suitable measures to overcome the disorder of the existing land policy system in China.
Strain-Controlled Fatigue Behavior and Microevolution of 316L Stainless Steel under Cyclic Shear Path
Authors: Liu Xinna , Zhang Shuai , Bao Yanmei , Zhang Zhongran , Yue Zhenming
Published: August 2022

Based on the twin bridge shear specimen, the cyclic shear experiments were performed on 1.2 mm thin plates of 316L metastable austenitic stainless steel with different strain amplitudes from 1 to 5% at ambient temperature. The fatigue behavior of 316L stainless steel under the cyclic shear path was studied, and the microscopic evolution of the material was analyzed. The results show that the cyclic stress response of 316L stainless steel exhibited cyclic hardening, saturation and cyclic softening, and the fatigue life is negatively correlated with the strain amplitude. The microstructure was analyzed by using electron back-scattered diffraction (EBSD). It was found that grain refinement and martensitic transformation during the deformation process led to rapid crack expansion and reduced the fatigue life of 316L.
An Overview of Some Nonpiezoelectric Properties of BaTiO3 Ceramics Doped by Eu Ions
Authors: Krupska-Klimczak Magdalena , Gwizd Przemyslaw , Jankowska-Sumara Irena , Sitko Dorota , Jeleń Piotr
Published: August 2022

Ferroelectric ceramics BaTiO3:x%Eu (x = 0, 0.1, 1, 2, 3) were synthesized by a conventional method. Structural investigation confirmed that all ceramics possessed tetragonal (P4mm) symmetries at room temperature for the undoped ceramics as well as for the doped ceramics. Furthermore, a slight downshifting of the Curie temperature (TC) with an increasing Eu3+ doping amount has been noted. The Raman spectra unveiled the existence of new modes for higher-doped BaTiO3:x%Eu (BTEx) which are related to local disorders and defects. The ferroelectric properties were found to depend on both doping and the microstructure. The electrocaloric effect was also studied for those ceramics. It was observed that ΔT decreases with doping; however, the temperature range of its occurrence widens considerably.
Electrochemical Evaluation of Protective Coatings with Ti Additions on Mild Steel Substrate with Potential Application for PEM Fuel Cells
Authors: Avram Diana N. , Davidescu Corneliu M. , Dan Mircea L. , Mirza-Rosca Julia C. , Hulka Iosif , Pascu Alexandru , Stanciu Elena M.
Published: August 2022

In this work, the corrosion behavior of NiCr(Ti) protective coatings deposited on mild steel substrates through laser cladding technology is studied as an alternative new material for metallic bipolar plates used in PEMFC applications. For electrochemical testing, a solution consisting of 0.5 M H2SO4 + 2 ppm F− at room temperature is used as an electrolyte. The fluoride ions are added to simulate the conditions in the PEM fuel cell due to degradation of the proton exchange membrane and fluoride release. A saturated calomel electrode (SCE) is used as a reference electrode and a platinum mesh as the counter electrode. Scanning electron microscopy (SEM) and optical microscopy (OM) are used for studying the morphology of the protective coatings and the effect of Ti addition. The electrochemical evaluation consisted of measuring the open circuit potential (OCP), followed by electrochemical impedance spectroscopy measurements (EIS) and potentiodynamic polarization. It is found that the coatings with 5% Ti, 7% Ti and 10% Ti addition comply with the conditions of the US DOE regarding corrosion performance to be used as materials for the manufacture of the bipolar plates.
Influence of Structural Porosity and Martensite Evolution on Mechanical Characteristics of Nitinol via In-Silico Finite Element Approach
Authors: Chekotu Josiah Cherian , Kinahan David , Goodall Russell , Brabazon Dermot
Published: August 2022

Nitinol (NiTi) alloys are gaining extensive attention due to their excellent mechanical, superelasticity, and biocompatibility properties. It is difficult to model the complex mechanical behavior of NiTi alloys due to the solid-state diffusionless phase transformations, and the differing elasticity and plasticity presenting from these two phases. In this work, an Auricchio finite element (FE) model was used to model the mechanical behavior of superelastic NiTi and was validated with experimental data from literature. A Representative Volume Element (RVE) was used to simulate the NiTi microstructure, and a microscale study was performed to understand how the evolution of martensite phase from austenite affects the response of the material upon loading. Laser Powder Bed Fusion (L-PBF) is an effective way to build complex NiTi components. Porosity being one of the major defects in Laser Powder Bed Fusion (L-PBF) processes, the model was used to correlate the macroscale effect of porosity (1.4–83.4%) with structural stiffness, dissipated energy during phase transformations, and damping properties. The results collectively summarize the effectiveness of the Auricchio model and show that this model can aid engineers to plan NiTi processing and operational parameters, for example for heat pump, medical implant, actuator, and shock absorption applications.
Study on Pyrolysis–Mechanics–Seepage Behavior of Oil Shale in a Closed System Subject to Real-Time Temperature Variations
Authors: Wang Lei , Su Jianzheng , Yang Dong
Published: August 2022

In situ mining is a practical and feasible technology for extracting oil shale. However, the extracted oil shale is subject to formation stress. This study systematically investigates the pyrolysis–mechanics–seepage problems of oil shale exploitation, which are subject to thermomechanical coupling using a thermal simulation experimental device representing a closed system, high-temperature rock mechanics testing system, and high-temperature triaxial permeability testing device. The results reveal the following. (i) The yield of gaseous hydrocarbon in the closed system increases throughout the pyrolysis reaction. Due to secondary cracking, the production of light and heavy hydrocarbon components first increases, and then decreases during the pyrolysis reaction. The parallel first-order reaction kinetic model shows a good fit with the pyrolysis and hydrocarbon generation processes of oil shale. With increasing temperature, the hydrocarbon generation conversion rate gradually increases, and the uniaxial compressive strength of oil shale was found to initially decrease and then increase. The compressive strength was the lowest at 400 °C, and the conversion rate of hydrocarbon formation gradually increased. The transformation of kaolinite into metakaolinite at high temperatures is the primary reason for the increase in compressive strength of oil shale at 400–600 °C. (ii) When the temperature is between 20 and 400 °C, the magnitude of oil shale permeability under stress is small (~10−2 md). When the temperature exceeds 400 °C, the permeability of the oil shale is large, and it decreases approximately linearly with increasing pore pressure, which is attributed to the joint action of the gas slippage effect, adsorption effect, and effective stress. The results of this research provide a basis for high efficiency in situ exploitation of oil shale.
Numerical Assessment of Damage Parameters for a Hard Interface Model
Authors: Raffa Maria Letizia , Rizzoni Raffaella , Lebon Frédéric
Published: August 2022

Adhesive interfaces are suitable modelling tools to describe very thin elastic layers and the related occurring phenomena (such as damage, viscosity, friction, etc.), without using a volumetric description, which is often computationally prohibitive in a large-scale numerical simulation. A major drawback of these kinds of models is the identification of free parameters, because of the smallness of a direct observation scale. This paper proposes a numerical assessment of two model parameters, a damage energy threshold and a damage viscosity, of a hard interface model previously formulated by authors. The proposed assessment protocol uses macroscopic experimental data, available in the literature, on structural adhesives under standard characterization tests. The numerical results obtained give insights into the physical interpretation of these parameters.
Magnetite Nanoparticles In-Situ Grown and Clustered on Reduced Graphene Oxide for Supercapacitor Electrodes
Authors: Jiang Yue , Han Jinxun , Wei Xiaoqin , Zhang Hanzhuo , Zhang Zhihui , Ren Luquan
Published: August 2022

Fe3O4 nanoparticles with average sizes of 3–8 nm were in-situ grown and self-assembled as homogeneous clusters on reduced graphene oxide (RGO) via coprecipitation with some additives, where RGO sheets were expanded from restacking and an increased surface area was obtained. The crystallization, purity and growth evolution of as-prepared Fe3O4/RGO nanocomposites were examined and discussed. Supercapacitor performance was investigated in a series of electrochemical tests and compared with pure Fe3O4. In 1 M KOH electrolyte, a high specific capacitance of 317.4 F g−1 at current density of 0.5 A g−1 was achieved, with the cycling stability remaining at 86.9% after 5500 cycles. The improved electrochemical properties of Fe3O4/RGO nanocomposites can be attributed to high electron transport, increased interfaces and positive synergistic effects between Fe3O4 and RGO.