bluuebunny/crossref_metadata_2025 huggingface dataset:
The Learning Curve of Robotic Thyroid Surgery and the Avoidance of Temporary Hypoparathyroidism after Total Thyroidectomy and Concomitant Central Compartment Node Dissection: A Single Surgeon’s Experience
Authors: Park Jae , Lee Jun , Cho Jae Won , Yoon Jong
Published: June 2019

The aim of this study was to evaluate the learning curve of robotic thyroid surgery with regard to both operation time and temporary hypoparathyroidism using quantitative statistical analysis. A total of 194 patients who underwent total thyroidectomy and concomitant central compartment node dissection for papillary thyroid carcinoma by a single surgeon between December 2008 and September 2017 were enrolled. The learning curve for operation time was assessed using the cumulative sum (CUSUM) technique, and the number of procedures required to reduce the incidence of temporary hypoparathyroidism to less than 30% was determined using the CUSUM and risk-adjusted CUSUM (RA-CUSUM) techniques. The learning curve for operation time was divided into three phases: phase 1 (the initial learning period, 1st–19th cases), phase 2 (the challenging period, 20th–121st cases), and phase 3 (the competent phase, 122nd–194th cases). To reduce the incidence of temporary hypoparathyroidism to <30% required 119 cases, and after adjustment for potential risk factors by RA-CUSUM analysis this extended to 173 cases. Technical proficiency for robotic thyroid surgery with respect to the avoidance of surgical complications probably requires a longer learning period than that required for operation time.
Analytical Model for the Structural Behavior of Pipelines During Lowering-In
Authors: Hwang Woongik , Lee Jong Seh
Published: June 2019

Since pipelines experience the largest deformation during lowering-in, structural analysis for this construction sequence should be performed to ensure structural safety. In this study, a new analytical model named the “segmental pipeline model” was developed to predict the structural behavior of the pipeline. This analytical model consists of several segmental elements to represent various boundary and contact conditions. Therefore, the segmental pipeline model can consider the geometric configuration and characteristics of pipelines that appear during lowering-in. Adopting the Euler-Bernoulli beam and two-parameter beam on elastic foundation theory, the new model takes the effect of the soil and axial forces acting on the pipelines into account. This paper compares the displacements, sectional bending moments and shear forces of the pipeline obtained from the analytical model and finite element (FE) analysis, where good agreement was demonstrated. Also, the paper presents three examples to demonstrate the applicability of the analytical model.
Use of Anionic Polysaccharides in the Development of 3D Bioprinting Technology
Authors: Tai Chia , Bouissil Soukaina , Gantumur Enkhtuul , Carranza Mary Stephanie , Yoshii Ayano , Sakai Shinji , Pierre Guillaume , Michaud Philippe , Delattre Cédric
Published: June 2019

Three-dimensional (3D) bioprinting technology is now one of the best ways to generate new biomaterial for potential biomedical applications. Significant progress in this field since two decades ago has pointed the way toward use of natural biopolymers such as polysaccharides. Generally, these biopolymers such as alginate possess specific reactive groups such as carboxylate able to be chemically or enzymatically functionalized to generate very interesting hydrogel structures with biomedical applications in cell generation. This present review gives an overview of the main natural anionic polysaccharides and focuses on the description of the 3D bioprinting concept with the recent development of bioprinting processes using alginate as polysaccharide.
Special Issue on Novel Insights into Orbital Angular Momentum Beams: From Fundamentals, Devices to Applications
Authors: Yue Yang , Huang Hao , Ren Yongxiong , Pan Zhongqi , Willner Alan E.
Published: June 2019

It is well-known now that angular momentum carried by elementary particles can be categorized as spin angular momentum (SAM) and orbital angular momentum (OAM) [...]
A Holistic Framework for Forecasting Transformative AI
Authors: Gruetzemacher Ross
Published: June 2019

In this paper we describe a holistic AI forecasting framework which draws on a broad body of literature from disciplines such as forecasting, technological forecasting, futures studies and scenario planning. A review of this literature leads us to propose a new class of scenario planning techniques that we call scenario mapping techniques. These techniques include scenario network mapping, cognitive maps and fuzzy cognitive maps, as well as a new method we propose that we refer to as judgmental distillation mapping. This proposed technique is based on scenario mapping and judgmental forecasting techniques, and is intended to integrate a wide variety of forecasts into a technological map with probabilistic timelines. Judgmental distillation mapping is the centerpiece of the holistic forecasting framework in which it is used to inform a strategic planning process as well as for informing future iterations of the forecasting process. Together, the framework and new technique form a holistic rethinking of how we forecast AI. We also include a discussion of the strengths and weaknesses of the framework, its implications for practice and its implications on research priorities for AI forecasting researchers.
Isoform-Specific Role of Akt in Oral Squamous Cell Carcinoma
Authors: Roy Nand Kishor , Monisha Javadi , Padmavathi Ganesan , Lalhruaitluanga H. , Kumar Nachimuthu Senthil , Singh Anuj Kumar , Bordoloi Devivasha , Baruah Munindra Narayan , Ahmed Gazi Naseem , Longkumar Imliwati , Arfuso Frank , Kumar Alan Prem , Kunnumakkara Ajaikumar B.
Published: June 2019

Protein kinase B (Akt) plays a very significant role in various cancers including oral cancer. However, it has three isoforms (Akt1, Akt2, and Akt3) and they perform distinct functions and even play contrasting roles in different cancers. Therefore, it becomes essential to evaluate the isoform-specific role of Akt in oral cancer. In the present study, an attempt has been made to elucidate the isoform-specific role of Akt in oral cancer. The immunohistochemical analysis of oral cancer tissues showed an overexpression of Akt1 and 2 isoforms but not Akt3. Moreover, the dataset of “The Cancer Genome Atlas” for head and neck cancer has suggested the genetic alterations of Akt1 and 2 tend to be associated with the utmost poor clinical outcome in oral cancer. Further, treatment of oral cancer cells with tobacco and its components such as benzo(a)pyrene and nicotine caused increased mRNA levels of Akt1 and 2 isoforms and also enhanced the aggressiveness of oral cancer cells in terms of proliferation, and clonogenic and migration potential. Finally, silencing of Akt1 and 2 isoforms caused decreased cell survival and induced cell cycle arrest at the G2/M phase. Akt1/2 silencing also reduced tobacco-induced aggressiveness by decreasing the clonogenic and migration potential of oral cancer cells. Moreover, silencing of Akt1 and 2 isoforms was found to decrease the expression of proteins regulating cancer cell survival and proliferation such as cyclooxygenase-2, B-cell lymphoma 2 (Bcl-2), cyclin D1, and survivin. Thus, the important role of Akt1 and 2 isoforms have been elucidated in oral cancer with in-depth mechanistic analysis.
Aggregate Recycling in Construction: Analysis of the Gaps between the Chilean and Spanish Realities
Authors: Díaz Marcos , Almendro-Candel María Belén , Blanco David , Jordan Manuel Miguel
Published: June 2019

This study conducts a comparative analysis between Chilean and Spanish reality in regard to the recycling of aggregates and their reuse in road works and urban roads. The current situation of both countries was reviewed through different sources of information such as technical and legal regulation, projects and executed works, to then validate them in Chile by way of interviews to various professionals in the field of urban and interurban road construction, as well as others belonging to government bodies. Spain has extensive experience on this issue, as it has a culture of recycling and reusing aggregates that has produced excellent results, a situation which was taken into account to compare it to Chile’s reality. The conclusion is that currently, in Chile, although the existence of recycled aggregate use is known, this is not the case on a technical level for professionals in the sector. It lacks a specific government body that is in charge of this issue and does not have appropriate infrastructure for its treatment. The materials with a promising future within the construction sector are the production of crushed granular bases and the creation of low-resistance concretes, which cannot be used, as laws that regulate them are still being drafted.
B Cells as an Immune-Regulatory Signature in Ovarian Cancer
Authors: Gupta Prachi , Chen Changliang , Chaluvally-Raghavan Pradeep , Pradeep Sunila
Published: June 2019

Increasing evidence suggests that the immune system plays a dynamic role in the progression of ovarian cancer, the deadliest gynecological malignancy worldwide. Accumulation of tumor-infiltrating lymphocytes has been associated with increased survival in ovarian cancer patients, and diverse interactions among immune cells in the tumor microenvironment determine tumor progression. While the regulatory functions of T cells among tumor-infiltrating lymphocytes are well defined and also involve therapeutic interventions, the role of B cells in ovarian cancer progression is still limited to their impact on survival. Recent studies have identified both pro- and anti-tumor responses of B cells in solid tumors, as different subsets of B cells play diverse roles in progression. Thus, in-depth characterization of B cell subtypes in each disease stage is crucial for understanding the importance and therapeutic potential of these cells in ovarian cancer. In this review, we summarize current knowledge about B cells in ovarian cancer and discuss emerging therapeutic interventions that could harness B cells to combat this deadly disease.
P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules
Authors: Willot Sébastien J.-P. , Tieves Florian , Girhard Marco , Urlacher Vlada B. , Hollmann Frank , de Gonzalo Gonzalo
Published: June 2019

A set of dual functional small molecules (DFSMs) containing different amino acids has been synthesized and employed together with three different variants of the cytochrome P450 monooxygenase P450BM3 from Bacillus megaterium in H2O2-dependent oxidation reactions. These DFSMs enhance P450BM3 activity with hydrogen peroxide as an oxidant, converting these enzymes into formal peroxygenases. This system has been employed for the catalytic epoxidation of styrene and in the sulfoxidation of thioanisole. Various P450BM3 variants have been evaluated in terms of activity and selectivity of the peroxygenase reactions.
Uprighting an Impacted Permanent Mandibular First Molar Associated with a Dentigerous Cyst and a Missing Second Mandibular Molar—A Case Report
Authors: Tsironi Konstantina , Inglezos Emmanouil , Vardas Emmanouil , Mitsea Anastasia
Published: June 2019

The purpose of this paper is to present a case of an impacted mandibular first molar associated with a dentigerous cyst and a missing mandibular second molar in an 11-year-old girl that was treated with combined surgical and orthodontic procedures. After clinical and radiographic evaluation, marsupialization of the cyst was decided, and a molar attachment was bonded on the buccal side of the impacted molar as a part of a full orthodontic treatment with fixed appliances. After 18 months of orthodontic traction, the molar was moved to a more advantageous position, and new bone apposition was observed on the site of the cystic lesion. Histological examination confirmed a dentigerous cyst. The molar was left to erupt spontaneously for 14 more months. A functional occlusion was finally achieved. An interdisciplinary approach proved to be an effective modality in treating a large dentigerous cyst associated with a deeply impacted first mandibular molar, presenting many advantages, such as new bone apposition and patient comfort.
Impact of Different Irrigant Agitation Methods on Bacterial Elimination from Infected Root Canals
Authors: Hage Wajih , De Moor Roeland J. G. , Hajj Désirée , Sfeir Germain , Sarkis Dolla K. , Zogheib Carla
Published: June 2019

Activation techniques are essential for root canal disinfection but may result in incomplete removal of bacteria. The aim of our study was to assess the antibacterial action of sonically, ultrasonically and laser-activated irrigation and 5.25% sodium hypochlorite (NaOCl) on Enterococcus faecalis in an infected tooth. Forty-four extracted mandibular premolars were mechanically prepared, sterilized, and inoculated with E. faecalis for 1 week. Bacterial counts after inoculation were evaluated in 4 randomly chosen teeth, remaining root canals were divided into 4 groups. Group A: laser-activated irrigation by photon-induced photoacoustic streaming, Group B: the sonic irrigation by EDDY, Group C: ultrasonic irrigation by EndoUltra, and Group D: 5.25% NaOCl. Colony forming unit (CFU) counts were measured and Kolmogorov–Smirnov, Wilcoxon, Kruskal–Wallis and Mann–Whitney tests were used to determine differences. The mean of CFU was found to significantly decrease in group D, 2110 ± 1015.93 (p < 0.001). Changes in measurement levels followed the same trend over time in groups A 27.40 ± 30.15, B 81.3 ± 85.68 and C 44.40 ± 67.12 (p = 0.141). The average CFU after irrigation in all groups was significantly greater than 0. Within the limitations of this study, all activation techniques were superior to NaOCl 5.25% in reducing E. faecalis from the infected tooth model.
Prediction of MoRFs in Protein Sequences with MLPs Based on Sequence Properties and Evolution Information
Authors: He Hao , Zhao Jiaxiang , Sun Guiling
Published: June 2019

Molecular recognition features (MoRFs) are one important type of intrinsically disordered proteins functional regions that can undergo a disorder-to-order transition through binding to their interaction partners. Prediction of MoRFs is crucial, as the functions of MoRFs are associated with many diseases and can therefore become the potential drug targets. In this paper, a method of predicting MoRFs is developed based on the sequence properties and evolutionary information. To this end, we design two distinct multi-layer perceptron (MLP) neural networks and present a procedure to train them. We develop a preprocessing process which exploits different sizes of sliding windows to capture various properties related to MoRFs. We then use the Bayes rule together with the outputs of two trained MLP neural networks to predict MoRFs. In comparison to several state-of-the-art methods, the simulation results show that our method is competitive.
Feasibility Study of Passive Bistatic Radar Based on Phased Array Radar Signals
Authors: Pan Jiameng , Hu Panhe , Zhu Qian , Bao Qinglong , Chen Zengping
Published: June 2019

This paper presents the concept of a passive bistatic radar (PBR) system using existing phased array radar (PAR) as the source of illumination. Different from PBR based on common civil illuminators of opportunity, we develop an experimental PBR system using an high-power air surveillance PAR with abundant signal modulation forms as the transmitter. After the introduction of the PBR system and PAR signals, it can be concluded that the agility of the waveform parameters of PAR signal brings two problems to the signal processing of the PBR systems, which are not discussed in conventional PBR systems. The first problem is the time and frequency synchronization of the system, so we propose a direct wave parameter estimation method based on template matching to estimate the parameters of the transmitted signal in real time to achieve time and frequency synchronization of the system. The second problem is the coherent integration for moving target detection and weak target detection, so we propose a coherent integration method based on Radon–Nonuniform Fast Fourier Transform (Radon-NUFFT) to deal with the problems introduced by the agile waveform parameters. Preliminary results from the field experiment demonstrate the feasibility of the PBR system based on PAR signals, and the effectiveness of the proposed methods is verified.
A Minimum Mean-Square-Error (MMSE) Decoder for Quasi-Orthogonal Space–Time Block Code
Authors: Jeong Jae Jin
Published: June 2019

The quasi-orthogonal space–time block code (QO-STBC) was introduced to achieve a full transmission rate for the four antennas system. In this paper, a decoding method for the QO-STBC is proposed to improve the bit-error-rate (BER) and to solve a rank-deficient problem. The proposed algorithm is based on the minimum mean-square-error (MMSE) technique. To overcome the implementation problem from the MMSE, an estimation method of the noise variance is developed in this paper. The proposed algorithm is implemented without matrix inversion, therefore, the proposed algorithm achieves a better BER than the conventional algorithms, as it has a low computational complexity. The simulation results show the low BER of the proposed algorithm in a Rayleigh fading channel.
Power Generation Performance of Building-Integrated Photovoltaic Systems in a Zero Energy Building
Authors: Choi Won Jun , Joo Hong Jin , Park Jae-Wan , Kim Sang-kyun , Lee Jae-Bum
Published: June 2019

In this study, the long-term operational performance of building-integrated photovoltaic (BIPV) systems was analyzed in the Carbon Zero Building of the National Institute of Environmental Research (NIER) of South Korea, with a total area of 2449 m2. Three types of BIPV modules (glass to glass, glass to Tedlar/crystal, and amorphous) were installed in the building envelopes (roofs, walls, windows, atrium, and pergola) with a total capacity of 116.2 kWp. Over a five-year period, the average annual energy production was 855.6 kWh/kWp, the system loss ranged from 0.14 to 0.31 h/d, and the capture loss ranged from 0.21 to 1.81 h/d. The causes of capture losses were degradation of the power generation efficiency of the horizontal installation module due to the accumulation of dust and reduced energy production due to application of the same inverter for the crystal system module and amorphous module. As a result, the BIPV systems with an installation angle of 30° exhibited approximately 57% higher energy production than vertically (90°) installed systems under the same solar radiation. Moreover, horizontal (0°) BIPV systems exhibited up to 14% higher energy production than vertical BIPV systems.
A New Coupling Solution for G3-PLC Employment in MV Smart Grids
Authors: Artale Giovanni , Cataliotti Antonio , Cosentino Valentina , Di Cara Dario , Fiorelli Riccardo , Guaiana Salvatore , Panzavecchia Nicola , Tinè Giovanni
Published: June 2019

This paper proposes a new coupling solution for transmitting narrowband multicarrier power line communication (PLC) signals over medium voltage (MV) power lines. The proposed system is based on an innovative PLC coupling principle, patented by the authors, which exploits the capacitive divider embedded in voltage detecting systems (VDS) already installed inside the MV switchboard. Thus, no dedicated couplers have to be installed and no switchboard modifications or energy interruptions are needed. This allows a significant cost reduction of MV PLC implementation. A first prototype of the proposed coupling system was presented in previous papers: it had a 15 kHz bandwidth useful to couple single carrier PSK modulated PLC signals with a center frequency from 50–200 kHz. In this paper, a new prototype is developed with a larger bandwidth, up to 164 kHz, thus allowing to couple multicarrier G3-PLC signals using orthogonal frequency division multiplexing (OFDM) digital modulation. This modulation ensures a more robust communication even in harsh power line channels. In the paper, the new coupling system design is described in detail. A new procedure is presented for tuning the coupling system parameters at first installation in a generic MV switchboard. Finally, laboratory and in-field experimental test results are reported and discussed. The coupling performances are evaluated measuring the throughput and success rate in the case of both 18 and 36 subcarriers, in one of the different tone masks standardized for the FCC-above CENELEC band (that is, from 154.6875–487.5 kHz). The experimental results show an efficient behavior of the proposed coupler allowing a two-way communication of G3-PLC OFDM signals on MV networks.
Diagnosis of Static Eccentricity in 3-Phase Synchronous Machines using a Pseudo Zero-Sequence Current
Authors: Gyftakis Konstantinos N. , Platero Carlos A. , Zhang Yucheng , Bernal Santiago
Published: June 2019

Large synchronous generators are the heart of the modern world, while producing the vast majority of the electric power consumed globally. Although they are robust devices, they are prone to degradation and failure. If such failures are not detected at an early stage, then the negative impact may be catastrophic in terms of financial costs, repair times, human lives and quality of life. This is the reason for continuous research in the field of condition monitoring aiming towards the reliable operation of synchronous generators. This paper proposes a novel technique for the diagnosis of the static eccentricity in synchronous generators. The proposed approach is off-line and non-intrusive, allowing the estimation of the fault severity with stator current measurements only. The performed work has been carried out with Finite Element Simulations and extensive experimental testing.
Effects of the Current Direction on the Energy Production of a Tidal Farm: The Case of Raz Blanchard (France)
Authors: Nguyen Van Thinh , Santa Cruz Alina , Guillou Sylvain S. , Shiekh Elsouk Mohamad N. , Thiébot Jérôme
Published: June 2019

This study aims to investigate the influence of the current direction on the energy production of a tidal turbines array. It is based on a three-dimensional (3D) numerical simulation of the flow where the turbines are represented with actuator disks. The case study consists of modelling the energy extraction of a small array of turbines (staggered and aligned layouts) placed in the Raz Blanchard (Alderney Race, France). The simulations are performed with hydrodynamic data (current magnitude and direction) representative of a mean tide, with several resistance forces and ambient turbulence intensities. The influence of the current direction on the energy production is highlighted by comparing the simulations forced with the real current direction with those in which the angle of incidence between the incoming flow and the turbine’s axis is “switched off” (bi-directional flow). When the flow is aligned with the turbines’ axis (misalignment “switched off”), the staggered layout produces more than the aligned arrangement. Comparison of the two types of simulations (misalignment switched off or not) shows that the misalignment of the flow around a predominant direction reduces the energy produced by the staggered layout and increases the production of the aligned layout. Furthermore, it suggests that the mean energy produced per machine is almost the same for both layouts. Higher turbulence intensity reduces the positive effect of the directional spreading on the aligned layout production and limits the negative effect on the staggered layout production.
Divergent Last Century Tree Growth along An Altitudinal Gradient in A Pinus sylvestris L. Dry-edge Population
Authors: Fernández-Pérez Laura , Zavala Miguel , Villar -Salvador Pedro , Madrigal-González Jaime
Published: June 2019

Research Highlights: This research highlights the importance of environmental gradients in shaping tree growth responses to global change drivers and the difficulty of attributing impacts to a single directional driver. Background and Objectives: Temperature increases associated with climate change might strongly influence tree growth and forest productivity in temperate forest species. However, the direction and intensity of these effects at the dry edge of species range are still unclear, particularly given the interaction between local factors and other global change drivers such as land use change, atmospheric CO2 increase and nitrogen deposition. While recent studies suggest that tree growth in cool temperate forests has accelerated during the last decades of the 20th century, other studies suggest a prevalence of declining growth, especially in dry-edge populations. Materials and Methods: Using historical forest inventories, we analyzed last century tree growth trends (1930–2010) along an elevation gradient (1350–1900 meters above sea level (m a.s.l.)) in a dry edge scots pine (Pinus sylvestris L.) forest in Central Iberian Peninsula. Growth was estimated as decadal volume increments in harvested trees of different size classes from 1930 to 2010 (1930–1940, 1939–1949, 1949–1959, 1959–1968, 1989–1999, 2000–2010). Results: Our results showed opposite growth trends over time depending on elevation. While tree growth has accelerated in the low end of the altitudinal gradient, tree growth slowed down at higher elevations (1624–1895 m a.s.l.). Moreover, the magnitude of growth reduction along the altitudinal gradient increased with tree age. Conclusions: Throughout the last 80 years, growth trends in a rear-edge Pinus. sylvestris forest has shown divergent patterns along an altitudinal gradient. Specifically, environmental conditions or other factors, may have become more adverse for growth at high altitudes and have improved at low altitudes. This suggests that local factors such as topography can modulate the impact of climate change on forest ecosystems.
Linking Dendrometry and Dendrochronology in the Dominant Azorean Tree Laurus azorica (Seub.) Franco
Authors: Matos Bárbara , Borges Silva Lurdes , Camarinho Ricardo , Rodrigues Armindo S. , Rego Ruben , Câmara Mariana , Silva Luís
Published: June 2019

As in many archipelagos, the Azorean primary forest was largely cleared and replaced by secondary forest and grassland, the Azorean tree Laurus azorica (Seub.) Franco being one of the dominant trees in the remaining natural forests. Dendrochronological and dendrometric studies in the Azores mainly focused on non-indigenous trees, either used for timber (e.g., Cryptomeria japonica D. Don) or considered as invasive (Pittosporum undulatum Vent.). Therefore, this study aims to describe the growth ring anatomy of L. azorica, and to understand the relationship between dendrometric traits (e.g., trunk diameter at breast height; tree height), and the number of growth rings. Growth ring anatomy was accessed by wood anatomical preparation of microcore samples while tree age estimation was based on growth ring counts in wood cores taken at breast height and at base. A total of 145 trees were sampled, resulting in 262 increment cores, at six representative stands of laurel forest in São Miguel Island (Azores). The wood anatomical analysis confirmed the presence of clear annual ring boundaries, and a high structural similarity towards Laurus novocanariensis Rivas Mart., Lousã, Fern.Prieto, E.Días, J.C.Costa & C.Aguiar. Age at tree base averaged 33 years, with 60% of the trees between 25 and 50 years old, and only about 15% above 50 years old. This suggests the existence of a secondary forest that is more recent than expected, probably due to human disturbance. The allometric models showed best fit when calculated by stand, suggesting the effect of local environmental conditions on growth rate. Radial growth rate was estimated at 0.68 cm·year−1. Given the known dominance of this species and the threats affecting natural forests, this baseline study will allow a better understanding of forest distribution and dynamics, and support a more effective forest management approach.
Acrylamide Content of Experimental Flatbreads Prepared from Potato, Quinoa, and Wheat Flours with Added Fruit and Vegetable Peels and Mushroom Powders
Authors: Crawford Lauren M. , Kahlon Talwinder S. , Wang Selina C. , Friedman Mendel
Published: June 2019

Flatbreads are a major food consumed worldwide. To mitigate an undesirable safety aspect of flatbreads that might be associated with the potentially-toxic compound acrylamide, we recently developed recipes using a variety of grains that resulted in the production of low-acrylamide flatbreads. To further enhance the functionality of flatbreads, we have developed, in this work, new experimental flatbreads using potato, quinoa, and wheat flours supplemented with peel powders prepared from commercial nonorganic and organic fruits and vegetables (apples, cherry tomatoes, melons, oranges, pepino melons, sweet potato yams), potato peels, and mushroom powders (Lion’s Mane, Hericium erinaceus; Reishi, Ganoderma lucidum; and Turkey Tail, Trametes versicolor). These additives have all been reported to contain beneficial compositional and health properties. The results of fortification of the baked flatbreads showed either no effect or increases in acrylamide content by unknown mechanisms. Since the additives did not increase the acrylamide content of the quinoa flour flatbreads for the most part, such supplemented quinoa flatbreads have the potential to serve as a nutritional, gluten-free, low-acrylamide, health-promoting functional food. Mushroom powder-fortified wheat flatbreads with relatively low acrylamide content may also have health benefits.
Valorization of Tomato Surplus and Waste Fractions: A Case Study Using Norway, Belgium, Poland, and Turkey as Examples
Authors: Løvdal Trond , Van Droogenbroeck Bart , Eroglu Evren Caglar , Kaniszewski Stanislaw , Agati Giovanni , Verheul Michel , Skipnes Dagbjørn
Published: June 2019

There is a large potential in Europe for valorization in the vegetable food supply chain. For example, there is occasionally overproduction of tomatoes for fresh consumption, and a fraction of the production is unsuited for fresh consumption sale (unacceptable color, shape, maturity, lesions, etc.). In countries where the facilities and infrastructure for tomato processing is lacking, these tomatoes are normally destroyed, used as landfilling or animal feed, and represent an economic loss for producers and negative environmental impact. Likewise, there is also a potential in the tomato processing industry to valorize side streams and reduce waste. The present paper provides an overview of tomato production in Europe and the strategies employed for processing and valorization of tomato side streams and waste fractions. Special emphasis is put on the four tomato-producing countries Norway, Belgium, Poland, and Turkey. These countries are very different regards for example their climatic preconditions for tomato production and volumes produced, and represent the extremes among European tomato producing countries. Postharvest treatments and applications for optimized harvest time and improved storage for premium raw material quality are discussed, as well as novel, sustainable processing technologies for minimum waste and side stream valorization. Preservation and enrichment of lycopene, the primary health promoting agent and sales argument, is reviewed in detail. The European volume of tomato postharvest wastage is estimated at >3 million metric tons per year. Together, the optimization of harvesting time and preprocessing storage conditions and sustainable food processing technologies, coupled with stabilization and valorization of processing by-products and side streams, can significantly contribute to the valorization of this underutilized biomass.
Population Genetic Divergence and Environment Influence the Gut Microbiome in Oregon Threespine Stickleback
Authors: Steury Robert A. , Currey Mark C. , Cresko William A. , Bohannan Brendan J. M.
Published: June 2019

Much of animal-associated microbiome research has been conducted in species for which little is known of their natural ecology and evolution. Microbiome studies that combine population genetic, environment, and geographic data for wild organisms can be very informative, especially in situations where host genetic variation and the environment both influence microbiome variation. The few studies that have related population genetic and microbiome variation in wild populations have been constrained by observation-based kinship data or incomplete genomic information. Here we integrate population genomic and microbiome analyses in wild threespine stickleback fish distributed throughout western Oregon, USA. We found that gut microbiome diversity and composition partitioned more among than within wild host populations and was better explained by host population genetic divergence than by environment and geography. We also identified gut microbial taxa that were most differentially abundant across environments and across genetically divergent populations. Our findings highlight the benefits of studies that investigate host-associated microbiomes in wild organisms.
Short-Term Effects of Carbonaceous Components in PM2.5 on Pulmonary Function: A Panel Study of 37 Chinese Healthy Adults
Authors: Huang Shichun , Feng Huan , Zuo Shanshan , Liao Jingling , He Mingquan , Shima Masayuki , Tamura Kenji , Li Yang , Ma Lu
Published: June 2019

Objectives: To explore the health effects of indoor/outdoor carbonaceous compositions in PM2.5 on pulmonary function among healthy students living in the local university campus. Methods: Daily peak expiratory flow (PEF) and forced expiratory volume in 1 second (FEV1) were measured among 37 healthy students in the morning and evening for four two-week periods. Concurrent concentrations of indoor and outdoor PM2.5 (particulate matter with an aerodynamic diameter ≤ 2.5μm), carbonaceous components in PM2.5, ambient temperature, and relative humidity in the study area were also obtained. Mixed-effects model was applied to evaluate the associations between carbonaceous components and lung function. Different lags for the carbonaceous components were investigated. Results: In single-pollutant model, a 10 μg/m3 increase of indoor and outdoor EC (elemental carbon) associated with −3.93 (95%CI: −6.89, −0.97) L/min and −3.21 (95%CI: −5.67, −0.75) L/min change in evening PEF at lag 0 day, respectively. Also, a 10 μg/m3 increase of indoor and outdoor POC (primary organic carbon) concentration was significantly associated with −5.82 (95%CI: −10.82, −0.81) L/min and −7.32 (95%CI: −12.93, −1.71) L/min change of evening PEF at lag 0 day. After adjusting total mass of PM2.5, indoor EC consistently had a significant adverse impact on evening PEF and FEV1 at lag3 day and a cumulative effect at lag0-3 day. Conclusions: This study suggests that carbonaceous components in PM2.5 indeed have impacts on pulmonary function among healthy young adults especially on evening PEF. Thus, the local mitigation strategies on pollution are needed.
Experimental Method of Emission Generation Calibration Based on Reference Liquids Characterization
Authors: Soulet Sébastien , Duquesne Marie , Toutain Jean , Pairaud Charly , Lalo Hélène
Published: June 2019

This work focuses on an experimental study of the influence of e-liquid composition on the mass of vaporized e-liquid after standardized emission generation using a U-SAV (Universal System for Analysis of Vaping) vaping machine. All the experiments were based on the use of a Cubis 1Ω clearomiser and on the standard protocol for electronic cigarettes emission generation. Currently, there is no standardized method available to calibrate the emission generations of electronic cigarettes. Since the e-liquid compositions are not always known, we propose a simple, practical, effective, and fast method of emission generation calibration. Therefore, this paper examines a major issue in this new and constantly evolving field, allowing the validation of the emission generation results. To our knowledge, this method is a novelty in our discipline and could be easily developed in laboratories. Pure propylene-glycol, glycerol, ethanol, and water and their mixtures (20 e-liquids) were tested as reference materials, allowing an e-liquids benchmarking and the characterization of 800 commercial e-liquids (with known and unknown compositions) at a fixed power and for one inhalation profile (3 s puff duration and 55 mL of puff volume). The influence of ethanol and/or water addition in the e-liquid was characterized.
New 20 m Progressive Shuttle Test Protocol and Equation for Predicting the Maximal Oxygen Uptake of Korean Adolescents Aged 13–18 Years
Authors: Lee Sang-Hyun , Song Jung-Ran , Kim Yang-Jung , Kim Su-Jin , Park Hyuk , Kim Chang-Sun , Kwak Hyo-Bum , Kang Ju-Hee , Park Dong-Ho
Published: June 2019

Background: Although several equations for predicting VO2max in children and adolescents have been reported, the validity of application of these equations to the Korean population has not been verified. The purpose of study was to develop and validate regression models to estimate maximal oxygen uptake (VO2max) using a newly developed 20 m progressive shuttle test (20 m PST) protocol in Korean male (n = 80, 15.3 ± 1.86 years) and female (n = 81, 15.5 ± 1.73 years) adolescents aged 13–18 years. Methods: The modified 20 m PST was performed and the VO2max was assessed in a sample of 161 participants. The participants underwent a treadmill test (TT) in the laboratory and the modified 20 m PST in a gymnasium. For the validation study, the participants performed the TT with a stationary metabolic cart and the 20 m PST with a portable metabolic cart once. In addition, they performed the 20 m PST two more times to establish test–retest reliability. Results: The mean VO2max (49.6 ± 8.7 mL·kg−1·min−1) measured with the potable metabolic cart was significantly higher than that measured in the graded exercise test with the stationary metabolic cart (46.6 ± 8.9 mL·kg−1·min−1, p < 0.001) using the new 20 m PST protocol. The standard error of the estimate (SEE) between these two measurements was 1.35 mL·kg−1·min−1. However, the VO2max derived from the newly developed equation was 46.7 ± 7.3 mL·kg−1·min−1 (p > 0.05) and the SEE was 2.90 mL·kg−1·min−1. The test and retest trials of the 20 m PST yielded comparable results (laps, r = 0.96; last speed, r = 0.93). Conclusions: Our data suggest that the new 20 m PST protocol is valid and reliable and that the equation developed in this study provides a valid estimate of VO2max in Korean male and female adolescents aged 13–18 years.
Specializing Nurses as An Indirect Education Program for Stoma Patients
Authors: García-Goñi Manuel
Published: June 2019

Education programs are beneficial for patients with different chronic conditions. Prior studies have examined direct education, where information is transferred directly to patients. In contrast, in this program, information is transferred directly to nurses who become specialists and transfer education individually to patients. Hence, this paper evaluates the impact of having specialist nurses for stoma patients at hospitals, as those nurses provide healthcare to patients but also inform and educate patients about their condition and needs. The analysis uses an observational study with ostomized patients in Spain at hospitals with and without specialist nurses, and measures health service utilization and health-related quality of life (HRQL), besides performing a cost analysis and a cost-effectiveness analysis at both types of hospitals. The results show that patients with access to specialist nurses self-manage better, present lower adverse events and a better evolution of HRQL, and significantly demand more consultations with specialist nurses and less to A&E, primary care or specialists, resulting in important savings for the health system. Consequently, specializing or hiring nurses to provide indirect education to stoma patients is cost-effective and highly beneficial for patients. This type of indirect education strategy might be considered for specific conditions with low incidence or difficulties in identifying target patients or delivering information directly to them.
Mapping Variation in Breast Cancer Screening: Where to Intervene?
Authors: Padilla Cindy M. , Painblanc François , Soler-Michel Patricia , Vieira Veronica M.
Published: June 2019

Small geographic areas with lower mammography screening participation rates may reflect gaps in screening efforts. Our objective was to use spatial analyses to understand disparities in mammography screening use and to identify factors to increase its uptake in areas that need it in Lyon metropolitan area, France. Data for screened women between the ages of 50 and 74 were analyzed. Census blocks of screened and non screened women were extracted from the mammography screening programme 2015–2016 dataset. We used spatial regression models, within a generalized additive framework to determine clusters of census blocks with significantly higher prevalence of non-participation of mammography screening. Smoothed risk maps were crude and adjusted on the following covariates: deprivation index and opportunistic screening. Among 178,002 women aged 50 to 74, 49.9% received mammography screening. As hypothesized, women living in highly deprived census blocks had lower participation rates compared to less deprived blocks, 45.2% vs. 51.4% p < 0.001. Spatial analyses identified four clusters, one located in an urban area and three in suburban areas. Moreover, depending on the location of the cluster, the influence came from different variables. Knowing the impact of site-specific risk factors seems to be important for implementing an appropriate prevention intervention.
Methodology to Derive Objective Screen-State from Smartphones: A SMART Platform Study
Authors: Katapally Tarun Reddy , Chu Luan Manh
Published: June 2019

Time on screens (screen time) on multiple digital devices (computers, mobile phones, tablets, television screens, etc.) due to varied motivations (work, leisure, entertainment, gaming, etc.) has become an integral part of population behaviour. However, a significant evidence gap exists in screen time accumulated over ubiquitous mobile devices such as smartphones. This study aimed to develop an accurate, reliable and replicable methodology to derive objective screen time (i.e., screen-state) from all types of citizen-owned smartphones. A convenience sample of 538 adults (≥18 years) from two largest urban centres in Saskatchewan, Canada (Regina and Saskatoon) was recruited in 2017 and 2018. Participants used a custom-built smartphone application to provide objective and subjective data. A novel methodology was developed to derive objective screen-state, and these data were compared with subjective measures. The findings showed that objective screen-state from smartphones can be derived and assessed across a range of cut-points that take into consideration varied measurement errors. When objective measures were compared with subjective reporting, the results indicated that participants consistently underreported screen time. This study not only provides a methodology to derive objective screen-state from ubiquitous mobile devices such as smartphones but also emphasises the need to capture context via subjective measures.
E-Cigarettes are More Addictive than Traditional Cigarettes—A Study in Highly Educated Young People
Authors: Jankowski Mateusz , Krzystanek Marek , Zejda Jan Eugeniusz , Majek Paulina , Lubanski Jakub , Lawson Joshua Allan , Brozek Grzegorz
Published: June 2019

E-cigarettes are often considered less addictive than traditional cigarettes. This study aimed to assess patterns of e-cigarette use and to compare nicotine dependence among cigarette and e-cigarette users in a group of highly educated young adults. From 3002 healthy adults, a representative group of 30 cigarette smokers, 30 exclusive e-cigarette users, and 30 dual users were recruited. A 25-item questionnaire was used to collect information related to the patterns and attitudes towards the use of cigarettes and e-cigarettes. The Fagerström test for nicotine dependence (FTND) and its adapted version for e-cigarettes were used to analyze nicotine dependence in each of the groups. The nicotine dependence levels measured with FTND were over two times higher among e-cigarette users (mean 3.5) compared to traditional tobacco smokers (mean 1.6; p < 0.001). Similarly, among dual users, nicotine dependence levels were higher when using an e-cigarette (mean 4.7) compared to using traditional cigarettes (mean 3.2; p = 0.03). Habits and behaviors associated with the use of e-cigarettes did not differ significantly (p > 0.05) between exclusive e-cigarette users and dual users. The findings suggest that e-cigarettes may have a higher addictive potential than smoked cigarettes among young adults.
Automatic (Tactile) Map Generation—A Systematic Literature Review
Authors: Wabiński Jakub , Mościcka Albina
Published: June 2019

This paper presents a systematic literature review that reflects the current state of research in the field of algorithms and models for map generalization, the existing solutions for automatic (tactile) map generation, as well as good practices for designing spatial databases for the purposes of automatic map development. A total number of over 500 primary studies were screened in order to identify the most relevant research on automatic (tactile) map generation from the last decade. The reviewed papers revealed many existing solutions in the field of automatic map production, as well as algorithms (e.g., Douglas–Peucker, Visvalingam–Whyatt) and models (e.g., GAEL, CartACom) for data generalization that might be used to transform traditional spatial data into the haptic form, suitable for blind and visually impaired people. However, it turns out that a comprehensive solution for automatic tactile map generation does not exist.
Differences in Mutational Profile between Follicular Thyroid Carcinoma and Follicular Thyroid Adenoma Identified Using Next Generation Sequencing
Authors: Borowczyk Martyna , Szczepanek-Parulska Ewelina , Dębicki Szymon , Budny Bartłomiej , Verburg Frederik A. , Filipowicz Dorota , Więckowska Barbara , Janicka-Jedyńska Małgorzata , Gil Lidia , Ziemnicka Katarzyna , Ruchała Marek
Published: June 2019

We aimed to identify differences in mutational status between follicular thyroid adenoma (FTA) and follicular thyroid cancer (FTC). The study included 35 patients with FTA and 35 with FTC. DNA was extracted from formalin-fixed paraffin-embedded (FFPE) samples from thyroidectomy. Next-generation sequencing (NGS) was performed with the 50-gene Ion AmpliSeq Cancer Hotspot Panel v2. Potentially pathogenic mutations were found in 14 (40%) FTA and 24 (69%) FTC patients (OR (95%CI) = 3.27 (1.22−8.75)). The number of mutations was higher in patients with FTC than FTA (p-value = 0.03). SMAD4 and STK11 mutations were present only in patients with FTA, while defects in FBXW7, JAK3, KIT, NRAS, PIK3CA, SMARCB1, and TP53 were detected exclusively in FTC patients. TP53 mutations increased the risk of FTC; OR (95%CI) = 29.24 (1.64–522.00); p-value = 0.001. FLT3-positivity was higher in FTC than in the FTA group (51.4% vs. 28.6%; p-value = 0.051). The presence of FLT3 and TP53 with no RET mutations increased FTC detectability by 17.1%, whereas the absence of FLT3 and TP53 with a presence of RET mutations increased FTA detectability by 5.7%. TP53 and FLT3 are candidate markers for detecting malignancy in follicular lesions. The best model to predict FTA and FTC may consist of FLT3, TP53, and RET mutations considered together.