bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolutionary Genomic Comparisons of Chelonid Herpesvirus 5 (chhv5) From Fibropapillomatosis-Afflicted Green (Chelonia Mydas), Olive Ridley (Lepidochelys Olivacea), And Kemp’s Ridley (Lepidochelys Kempii) Sea Turtles.
Authors: Whitmore Liam , Yetsko Kelsey , Farrell Jessica A. , Page-Karjian Annie , Daniel Whitney , Shaver Donna J. , Frandsen Hilary R. , Walker J. Shelby , Crowder Whitney , Bovery Caitlin , Rollinson Ramia Devon , Burkhalter Brooke , Ryan Elizabeth , Duffy David J.
Published: July 2021

The spreading global sea turtle fibropapillomatosis (FP) epizootic is threatening some of Earth’s ancient reptiles, adding to the plethora of threats faced by these keystone species. Understanding this neoplastic disease, and its likely aetiological pathogen, chelonid alphaherpesvirus 5 (ChHV5), is crucial to understand how the disease impacts sea turtle populations and species and the future trajectory of disease incidence. We generated 20 ChHV5 genomes, from three sea turtle species, to better understand the viral variant diversity and gene evolution of this oncogenic virus. We revealed previously underappreciated genetic diversity within this virus (with an average of 2,035 single nucleotide polymorphisms [SNPs], 1.54% of the ChHV5 genome) and identified genes under the strongest evolutionary pressure. Furthermore, we investigated the phylogeny of ChHV5 at both genome and gene level, confirming the propensity of the virus to be interspecific with related variants able to infect multiple sea turtle species. Finally, we revealed unexpected intra-host diversity, with up to 0.15% of the viral genome varying between ChHV5 genomes isolated from different tumours concurrently arising within the same individual. These findings offer important insights into ChHV5 biology and provide genomic resources for this oncogenic virus.
Assessment of a Nano-Docetaxel Combined Treatment for Head and Neck Cancer
Authors: Lee Gee Young , Mubasher Mohamed , McKenzie Tamra S. , Schmitt Nicole C. , Sebelik Merry E. , Flanagan Carrie E. , Cothran Maya B. , Green Hadiyah-Nicole
Published: July 2021

Objective: The combination of docetaxel (DTX) with Laser-Activated NanoTherapy (LANT), as a treatment for head and neck cancer (HNC) may enhance the therapeutic efficacy of lower doses of DTX, thereby minimizing the effective dosage, side effects and treatment times. Material and methods: Three HNSCC cell lines, Detroit 562, FaDu, and CAL 27, were treated with four combinations of DTX + LANT to evaluate DTX dose reduction and cell viability. Results: The 1 nM DTX + 5 nM LANT combination was the most effective treatment, increasing cell death over its corresponding DTX monotreatment with approximately 86.6%, 80.7%, and 92.1% cell death for Detroit 562, FaDu, and CAL 27, respectively. In Detroit 562, the 1 nM DTX + 5 nM LANT combination treatment resulted in the highest percentage of DTX dose reduction at 84.6%; in FaDu and CAL 27, the 0.5 nM DTX + 5 nM LANT combination treatment resulted in the highest percentage of DTX dose reduction at 78.2% and 82.4%, respectively. Conclusion: LANT may increase the therapeutic efficacy of DTX at significantly lower doses, which could improve patient outcomes.
Physical Activity, Mental Health and Wellbeing of Irish Adolescents during Covid-19 Restrictions. A Re-Issue of the Physical Activity and Wellbeing Study (PAWS)
Authors: Murphy John , McGrane Bronagh , Sweeney Mary Rose
Published: July 2021

Covid-19 restrictions impacted many people’s daily lives through infection, fear of infection and the implementation of restrictions on movement. Restrictions and fear of contamination impacted physical activity patterns activity and increased mental health issues globally across a variety of ages. This re-issue of a questionnaire sought to examine the impact of Covid-19 restrictions on frequency of physical activity, participation in sports, wellbeing and symptoms of anxiety and depression in Irish adolescents. 3,021 adolescents from 61 post-primary schools in the Republic of Ireland completed questionnaires. Consistent with a previous issue of the questionnaire, a minority of adolescents were found to meet the WHO’s physical activity guidelines (11.6% of males and 5.2% of females) although there were large decreases in 1st year males and females. Adolescents reporting elevated symptoms of depression increased from 39% to 46% with almost 3 in 5 females reporting symptoms of depression ranging from mild to extreme. Highest levels of wellbeing were found in adolescents who participated in 3 or more sports, although there was an 8% reduction in the amount of adolescents who participated in 3 or more sports. There were no changes in physical activity levels overall, despite changes within sub-groups and patterns of physical activity. There was a clear increase in symptoms of depression, with females impacted more than males. Previously active individuals were more likely to increase activity and therefore report higher levels of mental health while those who were less active were more likely to decrease activity and report lower mental health.
Module-Type Triboelectric Nanogenerators Capable of Harvesting Power from a Variety of Mechanical Energy Sources
Authors: Shin Jaehee , Ji Sungho , Yoon Jiyoung , Park Jinhyoung
Published: July 2021

In this study, we propose a module-type triboelectric nanogenerator (TENG) capable of harvesting power from a variety of mechanical energy sources. The potential energy and kinetic energy of water are used for the rotational motion of the generator module, and electricity is generated by the contact/separation generation mode between the two triboelectric surfaces inside the rotating TENG. Through the parametric design of the internal friction surface structure and mass ball, we optimized the output of the proposed structure. To magnify the power, experiments were conducted to optimize the electrical output of the series of TENG units. The electrical signal generated by the module-type TENG can be used as a sensor to recognize the strength and direction of various physical quantities, such as wind or earthquake vibrations.
Protective Effects of Xylopia aethiopica Fruit Ethanol Extract on Cadmium-Induced Inflammation and Dyslipidemia in Male Albino Rats
Authors: Folorunsho Ayodele Peter , Ibukun Akinloye Dorcas , Jaimiu Akamo Adio , Adejare Agboola David , Adeboye Akinloye Oluseyi
Published: July 2021

The wide use of cadmium (Cd) in industry causes great environmental health problems to humans and animals. The aim of this study was to investigate the protective effects of Xylopia aethiopica fruit ethanol extract (XAFEE) on cadmium-induced inflammation and dyslipidemia in male albino rats. Thirty albino rats weighing 120–180 g were randomly selected into six groups (n = 5): control rats (administered distilled water only), Cd alone group (10 mg/ kg bw), Cd + 150 mg/kgbw XAFEE, Cd + 300 mg/kgbw XAFEE, 150 mg/kgbw XAFEE and 300 mg /kgbw XAFEE groups after 2-week acclimatization. The experiment lasted for 21 days, after which blood sample was collected by cardiac puncture. Changes in tumor necrosis factor (TNF-α), interleukin 10 (IL-10), total cholesterol (TC), triacylglycerol (TAG), phospholipids and free fatty acids (FFAs) concentrations in serum were determined. The results of the present study indicated that Cd exposure remarkably increased (p < 0.05) the TC, TAG, phospholipids, FFAs and TNF-α concentrations, and significantly decreased IL-10 concentration (p < 0.05). These findings suggest that inflammatory changes and alterations in lipid metabolism might be one of the mechanisms underlying the subtle effects of Cd toxicity, “dyslipidemia.” XAFEE expressed protective role against toxic influence of Cd on affected parameters in rats. The results raised the possibility of Xylopia aethiopica fruit being considered as a condiment in soup, local drinks, supplements or herbs preparations in areas where there are chances of Cd exposure, occupationally or environmentally.
Green Housing or Green Gentrification?
Authors: Yeganeh Armin , McCoy Andrew , Agee Philip , Schenk Todd , Hankey Steve
Published: July 2021

Research on green-certified buildings has often been focused on the benefits of green standards, such as energy efficiency, smart growth, resource conservation, and health protection. Recent studies suggest the adoption of a reductionist sustainability planning language can turn green-certified houses into luxury goods, attracting White, prime-age, college-educated households with some pro-environmental attitudes who replace existing long-term, lower-income residents in core urban areas. While many factors may work together in driving neighborhood change and gentrification in cities, the question this study aims to address is to what extent the supply of green-certified units can affect neighborhood change and gentrification? We use Central Virginia’s Multiple Listing Service (MLS) housing market transactions data and the U.S. Census Bureau’s socioeconomic data to present the differential effect of new construction of market-rate, green-certified units in a natural experiment using difference-in-differences estimates. We find that neighborhoods that include new, green-certified units have experienced a statistically significant increase in population, supporting new construction and positively affecting house prices. We also detect some negative effects on minorities and minority owners, but these effects have not yet reached statistical significance. This study finds strong evidence of green housing providing the conditions that make areas ripe for gentrification, but more studies should follow up to better measure and generalize this finding.
Prioridade para futuras vacinações contra a Covid-19 no Brasil: os usuários de transporte público devem ser um grupo-alvo?
Authors: Sathler Douglas , Leiva Guilherme
Published: July 2021

Considerando o recente surgimento de vacinas eficazes contra a Covid-19 e os escassos recursos para atender imediatamente à maior parte da população mundial, as sociedades precisam definir a ordem em que os grupos de cidadãos terão acesso às novas vacinas. As campanhas de vacinação devem priorizar a imunização de indivíduos vulneráveis e encarregados de ajudar outras pessoas, reduzindo as perdas humanas e minimizando os danos sociais e econômicos. No Brasil, os usuários de transporte público nas grandes cidades apresentam altos níveis de vulnerabilidade, diante de fatores relacionados à configuração espacial, à organização dos sistemas de transporte e ao alto percentual de pessoas de baixo nível socioeconômico em grandes periferias que dependem exclusivamente do transporte público para acesso a empregos e serviços básicos. A imunização dos usuários do transporte público pode produzir efeitos práticos relevantes no combate à Covid-19 no Brasil, tais como economia de recursos públicos, redução do número de óbitos e maior eficiência no controle setorizado da doença nas cidades. Portanto, sugerimos que os formuladores de políticas devem considerar os usuários frequentes de transporte público das grandes cidades brasileiras como um grupo-alvo nas campanhas de vacinação, dando a esse grupo um certo nível de prioridade com base em um mapeamento de risco adequado em nível local.
Tracking Pandemic Severity Using Data on the Age Structure of Mortality: Lessons From the 1918 Influenza Pandemic in Michigan
Authors: Chandra Siddharth , Christensen Julia
Published: July 2021

Objectives. To test whether distortions in the age structure of mortality during the 1918 influenza pandemic in Michigan tracked the severity of the pandemic.

Methods. We calculated monthly excess deaths during the period of 1918 to 1920 by using monthly data on all-cause deaths for the period of 1912 to 1920 in Michigan. Next, we measured distortions in the age distribution of deaths by using the Kuiper goodness-of-fit test statistic comparing the monthly distribution of deaths by age in 1918 to 1920 with the baseline distribution for the corresponding month for 1912 to 1917.

Results. Monthly distortions in the age distribution of deaths were correlated with excess deaths for the period of 1918 to 1920 in Michigan (r = 0.83; P < .001).

Conclusions. Distortions in the age distribution of deaths tracked variations in the severity of the 1918 influenza pandemic.

Public Health Implications. It may be possible to track the severity of pandemic activity with age-at-death data by identifying distortions in the age distribution of deaths. Public health authorities should explore the application of this approach to tracking the COVID-19 pandemic in the absence of complete data coverage or accurate cause-of-death data.
Office of Management and Budget Racial/Ethnic Categories in Mortality Research: A Framework for Including the Voices of Racialized Communities
Authors: Hayes-Bautista David E. , Bryant Mara , Yudell Michael , Hayes-Bautista Teodocia Maria , Partlow Keosha , Popejoy Alice Beecher , Burchard Esteban , Hsu Paul
Published: July 2021

Since its founding, the US government has sorted people into racial/ethnic categories for the purpose of allowing or disallowing their access to social services and protections.

The current Office of Management and Budget racial/ethnic categories originated in a dominant racial narrative that assumed a binary biological difference between Whites and non-Whites, with a hard-edged separation between them. There is debate about their continued use in researching group differences in mortality profiles and health outcomes: should we use them with modifications, cease using them entirely, or develop a new epistemology of human similarities and differences?

This essay offers a research framework for including in these debates the daily lived experiences of the 110 million racialized non-White Americans whose lived experiences are the legacy of historically limited access to society’s services and protections. The experience of Latinos in California is used to illustrate the major elements of this framework that may have an effect on mortality and health outcomes: a subaltern fuzzy-edged multivalent racial narrative, agency, voice, and community and cultural resilience.
Volar Plate Fixation Versus Cast Immobilization in Acceptably Reduced Intra-Articular Distal Radial Fractures
Authors: Selles C.A. , Mulders M.A.M. , Winkelhagen J. , van Eerten P.V. , Goslings J.C. , Schep N.W.L. ,   the VIPAR Collaborators
Published: July 2021

Background:

The evidence for the treatment of acceptably reduced intra-articular distal radial fractures remains inconclusive. We therefore compared the functional outcomes of cast immobilization (nonoperative) and volar plate fixation (operative) for patients with these fractures.

Methods:

This multicenter randomized controlled trial enrolled patients between 18 and 75 years old with an acceptably reduced intra-articular distal radial fracture. Patients were randomized to nonoperative treatment or to operative treatment. The primary outcome measure was the Patient-Rated Wrist Evaluation (PRWE) score after 12 months. Secondary outcome measures were the Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire; the Short Form-36 (SF-36) questionnaire; a visual analog scale for pain; range of motion; grip strength; radiographic parameters; and complications. Analyses followed the intention-to-treat principle.

Results:

A total of 96 patients were randomized, and 90 (46 in the nonoperative group and 44 in the operative group) were included in the analysis. Patients treated in the operative group had significantly better functional outcomes measured with the PRWE at 6 weeks, 3 months, 6 months, and 1 year. Additionally, a 28% rate of subsequent surgery was identified in the nonoperative group.

Conclusions:

Adult patients with an acceptably reduced intra-articular distal radial fracture have better functional outcomes for 12 months when treated operatively instead of nonoperatively. We therefore recommend surgical treatment for patients with these fractures.

Level of Evidence:

Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
Capsular Repair During Hip Arthroscopy Demonstrates Restoration of Axial Distraction Resistance in an in Vivo Intraoperative Testing Model
Authors: O’Neill Dillon C. , Tomasevich Kelly M. , Mortensen Alexander J. , Featherall Joseph , Ohlsen Suzanna M. , Aoki Stephen K.
Published: July 2021

Background:

Cadaveric models demonstrate that failure of hip capsular repair is dependent on the robustness of the repair construct. In vivo data on capsular repair construct efficacy are limited. We investigated the effect of a figure-of-8 capsular repair on hip distraction resistance relative to native and post-capsulotomy states. We hypothesized that an unrepaired capsulotomy would demonstrate increased axial distraction compared with the native state and that capsular repair would restore distraction resistance to native levels.

Methods:

Patients undergoing primary hip arthroscopy by a single surgeon were prospectively enrolled between March 2020 and June 2020. Prior to any instrumentation, fluoroscopic images of the operative hip were obtained at 12.5-lbs (5.7-kg) traction intervals, up to 100 lbs (45.4 kg). Anterolateral, modified anterior, and distal anterolateral portals were established. Following interportal capsulotomy, labral repair, and osteochondroplasty, fluoroscopic images were reobtained at each traction interval. Capsular repair was performed with use of a figure-of-8 suture configuration. Traction was reapplied and fluoroscopic images were again obtained. Joint distraction distance was measured at each traction interval for all 3 capsular states. Anteroposterior pelvic radiographs were utilized to scale fluoroscopic images to obtain joint space measurements in millimeters.

Results:

A total of 31 hips in 31 patients were included. Capsulotomy resulted in significant increases in distraction distance from 25 (11.3 kg) to 100 lbs of traction compared with both native and capsular repair states (all comparisons, p ≤ 0.017). Capsular repair yielded a significantly greater distraction distance compared with the native state at 37.5 lbs (17.0 kg; 5.49 versus 4.98 mm, respectively; p = 0.012) and 50 lbs (22.7 kg; 6.08 versus 5.35 mm; p < 0.001). The mean difference in distraction distance between native and capsular repair states from 25 to 100 lbs of traction was 0.01 mm.

Conclusions:

This in vivo model demonstrates that an unrepaired interportal capsulotomy significantly increases axial distraction distance compared with the native, intact hip capsule. Performing a complete capsular closure reconstitutes resistance to axial distraction intraoperatively. Future research should evaluate the in vivo effects and associated clinical outcomes of other published capsular repair techniques and assess the durability of capsular repairs over time.
Physics calculator application with matlab as a learning media to thermodynamics concept
Authors: Utari Kintan , Mulyaningsih Neng Nenden , Astuti Irnin Agustina Dwi , Bhakti Yoga Budi , Zulherman Zulherman
Published: July 2021

In the material of thermodynamics, many physics equations make students abstract in understanding the material. It is necessary to have a learning media to be able to visualize the thermodynamics material. Matlab is usually used in numerical analysis. Currently, Matlab can be used as a medium for learning physics by students to understand the material well. This study aims to develop a physics calculator application on the subject of thermodynamics using Matlab software. The research method used in this research is the research development method with the development model of ADDIE (Analysis, Design, Development, Implementation and Evaluation). The instruments used in this study were a validation test questionnaire and a student response questionnaire. The analysis technique applied is the descriptive analysis and the average analysis techniques. The results developed in this study are in the form of a Physics calculator application that helps physics calculations on thermodynamic material. The average validation result by material experts and media experts is 83.1%, with the "Good" category. In other words, according to experts, this media is suitable for use in physics learning. This application also received a positive response from students, with an average student response score of 79.8%. This application is also equipped with materials, physics calculators, physics simulations and question exercises so that it is expected to help students understand physics learning in thermodynamics material.
Needs analysis of physics e-module based on hybrid-PBL model on critical thinking skills improvement
Authors: Rahmawati Fitri , Sarwanto Sarwanto , Budiawanti Sri
Published: July 2021

The purpose of this study was to determine the need for teaching materials in the form of physics e-module based on Hybrid-PBL model in schools. This research applied a descriptive method. Data were collected using a needs analysis questionnaire with 14 respondents of physics teachers spread across several regions in Indonesia and 36 respondents of class XI students in SMA Negeri 2 Sragen. Based on the results of the questionnaire, the teachers indeed need teaching materials in the form of e-module and all teachers also agree that the Hybrid-PBL model can be applied in physics learning. Students are also interested in learning physics using teaching materials that can display images, videos, animations, and simulations as well as learning by involving problems in the surrounding environment. So it can be concluded that teachers and students need a physics e-module based on Hybrid-PBL model.
A Hashing Power Allocation Game with and without Risk-free Asset
Authors: Cheng Yukun , Du Donglei , Han Qiaoming
Published: July 2021

Miners in various blockchain-backed cryptocurrency networks compete to maintain the validity of the underlying distributed ledgers to earn the bootstrapped cryptocurrencies. With limited hashing power, each miner needs to decide how to allocate their resource to different cryptocurrencies so as to achieve the best overall payoff. Together all the miners form a hashing power allocation game. We consider two settings of the game, depending on whether each miner can allocate their fund to a risk-free asset or not. We show that this game admits unique pure Nash equilibrium in closed-form for both settings.
The Depth to Magnetic Sources in the Arctic and ItsRelationshipwith Some Parameters of the Lithosphere
Authors: Seredkina A.I. , Filippov S.V.
Published: July 2021

––The depth to magnetic sources in twenty Arctic tectonic provinces is determined from azimuthally averaged Fourier power spectra of geomagnetic anomalies according to the EMAG2v3 and WDMAM 2.0 global models. The resulting depths to the centroid and bottom of the magnetic lithosphere are more reliable than the depth to the upper magnetic boundary. The depth to the bottom of magnetic sources, corresponding to the Curie point depth, varies from 25.3 to 38.1 km in different provinces. The Curie point depth estimates are correlated with several parameters of the lithosphere. They are directly proportional to the lithospheric thickness and inversely proportional to average upper mantle temperatures, but the relationship with the intensity of long-wavelength satellite magnetic anomalies and crustal thickness is poor. The magnetic sources are located at crustal depths in most of the provinces, but the upper mantle may be magnetic beneath deep-water oceanic basins and the Laptev Sea. The results for the Laptev Sea shelf support a passive mechanism of current lithospheric extension in the area.
Palinostratigraphy and Paleoenvironments in the Gorodishchi Section (Middle Volga River Region, Kimmeridgian–Hauterivian)
Authors: Pestchevitskaya E.B.
Published: July 2021

—Eight dinocyst-based and three spore- and pollen-based biostratigraphic units are defined in the Kimmeridgian, Volgian, and Hauterivian of the Gorodishchi section, based on a biostratigraphic analysis of the successions of marine and terrestrial palynomorphs. Algological assemblages are described in more detail, and additional criteria for the definition of dinocyst zones established by previous researches are given. A more detailed biostratigraphic subdivision of the middle part of the Volgian is proposed. A local dinocyst zone in the Hauterivian and a biostratigraphic succession of spore–pollen units in the entire section are described for the first time. The research results demonstrate that the boundaries of many palynostratigraphic units exhibit a considerable correlation potential. Based on a biofacies analysis of the microphytoplankton, the dynamics of transgressive–regressive events is studied in relation to the accompanying oxygen and trophic conditions. Possible relationships between marine paleoenvironments and climatic changes reconstructed on the basis of spore–pol­len data are discussed.
A Revised Gassmann Fluid Replacement Model Based on Neutron-Density Logs in Shaly Sandstone Reservoirs
Authors: Chakraborty Shantanu , Mondal Samit , Chatterjee Rima
Published: July 2021

Fluid-replacement modeling (FRM) is a fundamental step in rock physics scenario modeling. The results help to conduct forward modeling for prediction of seismic signatures. Further, the analysis of the results improves the accuracy of quantitative interpretation and leads to an updated reservoir characterization. While modeling for different possible reservoir pore fluid scenarios, the quality of the results largely depends on the accuracy of the FRM. Gassmann (1951)fluid-replacement modeling (GFRM) is one of the widely adopted methods across the oil and gas industry. However, the Gassmann method assumes the reservoir as clean sandstone with connected pores. This causes Gassmann fluid-replacement results to overestimate the fluid effect in shaly sandstones. This study uses neutron and density logs to correct the overestimated results in shaly sandstone reservoirs. Due to the nature of these recordings, both of these log readings have close dependencies on the presence of shale. When the logs are plotted in a justified scale, the differences between the logs provide an accurate measurement of shaliness within the reservoir. The study has formulated a weight factor using the logs, which has further been used to scale the overestimated Gassmann-modeled fluid effect. The results of the revised method are independent of type of clay presence and associated effective porosity. Moreover, the corrected FRM results from the revised Gassmann method shows good agreement with rock physical interpretation of shaly sandstone reservoirs.
Integration of Extended Reality and a High-fidelity Simulator in Team-based Simulations for Arrhythmia Scenarios Among Undergraduate Nursing Students
Authors: Lee Youngho , Kim Sun Kyung , Yoon Hyoseok , Choi Jongmyung , Kim Hyesun
Published: July 2021

Objective: The purpose of this study was to evaluate the usability and feasibility of smart glasses for team-based simulations constructed using a high-fidelity simulator. Methods: Two scenarios of patients with arrhythmia were developed to establish the process for interprofessional interaction via smart-glass in 15 hour simulation training. Three to four participants made a team and took roles of either remote supporter and bad-side trainee with smart-glass. Usability, attitudes towards interprofessional health care team and learning satisfaction were assessed. A scale for software evaluation was used to obtain quantitative data and essay questionnaire were used to assess usability and feasibility of current program. Using SPSS (version 25.0), statistical analysis was conducted. Results: Data were obtained from 31 participants. The current program was given an easy to use (3.61±0.95) rating, and the users reported feeling confident during use (3.90±0.87). Participants responded positively to long-term use (3.26±0.89) and low levels of physical discomfort (1.96±1.06). The likability of the smart glasses-based simulation was high, with a high rating for satisfaction (4.65±0.55). The majority (84%) of subjects provided favorable responses to their overall experience with high expectations for improving safety in future clinical practice. Key barriers and challenges of the current program include unstable internet connection, poor resolution and display, and physical discomfort of the smart glasses with accessories. Conclusion: We determined the feasibility and acceptability of smart glasses for interprofessional interaction within a team-based simulation environment. Participants responded favorably toward a smart glasses-based simulation learning environment that would be applicable in clinical settings.
Hybrid SDN Networks: A multi-parameter server load balancing scheme
Authors: Malbašić Teodor , Bojović Petar D. , Bojović Živko , Šuh Jelena , Vujošević Dušan
Published: July 2021

Software-defined networking (SDN) provides many benefits, including traffic programmability, agility, and network automation. However, budget constraints burdened with technical (e.g., scalability, fault tolerance, security issues) and, sometimes, business challenges (user acceptance and confidence of network operators) make providers indecisive for full SDN deployment. Therefore, incremental deployment of SDN functionality through the placement of a limited set of SDN devices among traditional devices represents a rational and efficient environment that can offer customers modern and more data-intensive services. However, while hybrid SDN provides many benefits, it also has specific challenges addressed in the literature. This paper answers one of these challenges by presenting the research and development of a new load balancing scheme in the hybrid SDN environment built with a minimal SDN device set (controller and one switch). We propose a novel load balancing scheme to monitor current server load indicators and apply multi-parameter metrics for scheduling connections to balance the load on the servers as efficiently as possible. The base of the new load balancing scheme is continuous monitoring of server load indicators and implementations of multi-parameter metrics (CPU load, I/O Read, I/O Write, Link Upload, Link Download) for scheduling connections. The testing performed on servers aims to balance the server's load as efficiently as possible. The obtained results have shown that this mechanism achieves better results than existing load balancing schemes in traditional and SDN networks. Moreover, a proposed load balancing scheme can be used with various services and applied in any client-server environment.
Comprehensive Analysis of the Autophagy-dependent Ferroptosis-related Gene FANCD2 in Lung Adenocarcinoma
Authors: Miao Huikai , Ren Qiannan , Li Hongmu , Zeng Mingyue , Chen Dongni , Chen Youfang , Wen Zhesheng
Published: July 2021

Background. The development of lung adenocarcinoma (LUAD) involves the interactions between cell proliferation and death. Autophagy-dependent ferroptosis, a distinctive cell death process was implicated in a multitude of diseases, whereas no research revealing the relationship between autophagy-dependent ferroptosis and LUAD pathogenesis was reported. Thus, the primary objective was to explore the role and potential function of the autophagy-dependent ferroptosis-related genes in LUAD.Methods. Clinical information and transcriptome profiling of patients with LUAD were retrieved and downloaded from open-source databases. Autophagy-dependent ferroptosis-related genes were screened by published articles. The key gene was identified as the intersection between the differentially expressed genes and prognosis-related genes. Patients were divided into high- and low-risk groups using the expression level of the key gene. The validity of the key gene prognosis model was verified by survival analysis. The correlation between the clinical characteristics of LUAD and the expression level of the key gene was analyzed to explore the clinical significance and prognosis value. And the roles of the key gene in response to chemotherapy, immune microenvironment and tumor mutation burden were predicted. The validation of key gene expression levels were further performed by quantitative real-time PCR and immunohistochemistry staining.Results. FANCD2, a key autophagy-dependent ferroptosis-related gene by searching database, was confirmed as an independent prognostic factor for LUAD occurrence. The high expression level of FANCD2 was associated with an advantaged TNM stage, a less chemotherapy sensitivity, a low ImmuneScore, which indicated a deactivation status in immune microenvironment, a high tumor mutation burden, and a poor survival for LUAD patients. Pathway enrichment analysis showed that FANCD2 were involved in response to oxidative stress and neutrophil mediated immunity. Quantitative real-time PCR and immunohistochemistry staining showed that the expression level of FANCD2 is higher in LUAD patients than normal tissue samples, which was in accordance with the database report.Conclusion. FANCD2, a key gene related to autophagy-dependent ferroptosis, could work as a biomarker, which predicts the survival, chemotherapy sensitivity, tumor immunity and mutation burden of LUAD. Research of autophagy-dependent ferroptosis and targeting the FANCD2 may offer a new perspective for the treatment and improvement of prognosis in LUAD.
Blood Circulatory Level of Seven Sirtuins in Alzheimer’s Disease: Potent Biomarker Based on Translational Research
Authors: Pradhan Rashmita , Singh Abhinay Kumar , Kumar Pramod , Bajpai Swati , Pathak Mona , Chatterjee Prasun , Dwivedi Sadanand , Dey A.B. , Dey Sharmistha
Published: July 2021

Alzheimer's disease (AD) is an accelerating neurodegenerative disorder. Dysfunction of mitochondria and oxidative stress contributes to the pathogenesis of AD. Sirtuins play a role in this pathway and can be a potential marker to study neurodegenerative changes. This study evaluated serum levels of all seven sirtuins (SIRT1 – SIRT7) proteins in three study groups: AD, Mild Cognitive Impairment (MCI), and Geriatric Control (GC) by Surface Plasmon Resonance (SPR) technique. Further, it was validated by the Western blot experiment. ROC analysis was performed to differentiate the study group based on the concentration of serum SIRT proteins. Out of seven sirtuins, serum SIRT1, SIRT3, and SIRT6 levels (mean+SD) were significantly decreased in AD (1.65±0.56, 3.15±0.28, 3.36±0.32ng/µl); compare to MCI (2.17±0.39, 3.60±0.51, 3.73±0.48ng/µl) and GC (2.84±0.47, 4.55±0.48, 4.65±0.55ng/µl). ROC analysis showed the cut-off value with high sensitivity and specificity for cognitive impairment (AD and MCI). The concentration declined significantly with the disease progression. No specific difference was observed in case of other SIRTS between the study groups. This study reveals an inverse relation of serum SIRT1, SIRT3, and SIRT6 concentration with AD. ROC analysis showed that these serum proteins have greater accuracy in diagnosing of AD. This is the first report of estimation of all seven serum sirtuins and the clinical relevance of SIRT3 and SIRT6 as serum protein markers for AD.
Amyloid PET Quantification Using Low-dose CT-guided Anatomic Standardization
Authors: Matsuda Hiroshi , Yamao Tensho , Shakado Mitsuru , Shigemoto Yoko , Okita Kyoji , Sato Noriko
Published: July 2021

BackgroundCentiloid (CL) scaling has become a standardized quantitative measure in amyloid PET because it facilitates the direct comparison of results across institutions, even when different analytical methods or tracers are used. Standard volumes of interest must be used to calculate the CL scale after the anatomic standardization of amyloid PET images using coregistered MRI; if the MRI is unavailable, the CL scale cannot be accurately calculated. This study sought to determine the substitutability of low-dose CT, which is used to correct PET attenuation in PET/CT equipment, by evaluating the measurement accuracy when low-dose CT is used as an alternative to MRI in the calculation of the CL scale. Amyloid PET images obtained using 18F-flutemetamol from 24 patients with possible or probable Alzheimer’s disease were processed to calculate the CL scale using 3D T1-weighted MRI and low-dose CT of PET/CT. CLMRI and CLCT were respectively defined as the use of MRI and CT for anatomic standardization and compared. Trial registration: Japan Registry of Clinical Trials, jRCTs031180321. Registered 18 March 2019, https://jrct.niph.go.jp/latest-detail/jRCTs031180321.ResultsA Bland–Altman plot showed that CLCT was slightly but significantly underestimated (mean ± standard deviation, −1.7 ± 2.4; p < 0.002) compared with CLMRI. The 95% limits of agreement ranged from −2.8 to −0.7. Pearson correlation analysis showed a highly significant correlation of r = 0.998 between CLCT and CLMRI (p < 0.001). The linear regression equation was CLMRI = 1.027 × CLCT + 0.762. In a Bland-Altman plot, Spearman correlation analysis did not identify a significant association between the CLMRI versus CLCT difference and the CL load (ρ = −0.389, p = 0.060). This slight underestimation of CLCT may derive from slightly higher uptake when the cerebellum is used as a reference area in CT-based anatomically standardized PET images versus MRI-based images.ConclusionsLow-dose CT of PET/CT can substitute for MRI in the anatomic standardization used to calculate the CL scale from amyloid PET, although a slight underestimation occurs.
Inverted ternary OPD based on PEIE
Authors: An Tao , Liu Suiyang
Published: July 2021

This paper proposes an inverted ternary organic photodetector (OPD), whose structure is ITO/PEIE/PC61BM/P3HT:PCPDTBT/MoO3 /Al. The use of PEIE as the cathode buffer layer avoids the influence of acidic PEDOT:PSS on the surface and life of the conventional device . The preparation of the ternary active layer ensures the photoelectric characteristics of the device in the visible-infrared broad spectrum range. In this experiment, the effect of PEIE thickness on the working mode of the device was studied by changing the concentration of the spin-coated PEIE solution. Finally, when the solution of PEIE is less than 0.45wt%, the device works in the diode mode, on the contrary, it works in the photoconductive mode. And under 550nm illumination (optical power 4.02mW/cm2) , the device achieves a responsivity of 1.64A/W and an external quantum efficiency of 370%.
Efficacy of Concurrent Chemoradiotherapy In Subgroups of Stage III Nasopharyngeal Carcinoma: An Analysis Based On 10-Year Follow-Up
Authors: Wang Lei , Zheng Wu , Cheng Wanqin , Xie Dehuan , Lin Feifei , Xia Liangping , Su Yong
Published: July 2021

PurposeTo evaluate the efficacy of concurrent chemoradiotherapy (CCRT) in subgroups of stage III nasopharyngeal carcinoma (NPC) in the context of intensity-modulated radiotherapy (IMRT).Methods272 patients with stage III NPC who underwent IMRT with or without concurrent chemotherapy (CCT) were retrospectively reviewed. Clinicopathological features were evaluated by a Cox regression model to identify independent prognostic factors. Survival outcomes were assessed using Kaplan-Meier method and log-rank test.ResultsThe median follow-up time was 108 months. The 10-year locoregional-free survival (LRFS), distant metastasis-free survival (DMFS), disease-free survival (DFS), and overall survival (OS) rates were 87.8%, 80.7%, 68.8%, and 74.9%, respectively. Multivariate analysis showed that the N classification was significantly associated with DMFS (hazard ratio [HR] 3.616, 95% confidence interval [CI] 1.387-9.428, P=0.009), DFS (HR 2.417, 95% CI 1.291-4.423, P=0.006), and OS (HR 3.024, 95% CI 1.385-6.602, P=0.005). In patients with T1-3N2 disease, CCRT was associated with improved 10-year LRFS (89.6% vs. 65.4%, P=0.005), DFS (71.9% vs. 39.4%, P=0.001) and OS (80.0% vs. 50.5%, P=0.004) compared with IMRT alone. However, in patients with T3N0-1 disease, no significant survival differences were observed between patients treated with IMRT alone and CCRT (P>0.05). ConclusionCCRT is an effective therapy in stage III NPC, especially for patients with N2 disease but N0-1 disease. Individualized treatment strategies are essential for patients with varying disease risks.
Cesarean Delivery on Maternal Request and Common Child Health Outcomes: A Prospective Cohort Study
Authors: Si Ke-yi , Li Hong-tian , Zhou Yu-bo , Li Zhi-wen , Zhang Le , Zhang Ya-li , Ye Rong-wei , Liu Jian-meng
Published: July 2021

Cesarean delivery (CD) versus vaginal delivery was reported to increase the risks of childhood obesity, pneumonia, anemia, and neurobehavioral disorders, but few studies were able to deal with the confounding biases associated with medical conditions indicating cesareans. This prospective cohort study aims to investigate the associations of non-medically indicated CD on maternal request (CDMR) with multiple child health outcomes. Among live-born infants whose mothers participated in a randomized controlled trial on micronutrient supplementation and pregnancy outcomes during 2006-2009 in 5 rural counties in Hebei Province, China, 6972 singletons born by full-term spontaneous vaginal delivery (SVD) and 3626 by CDMR were selected and followed up at 1.5-5 years in 2011. The primary outcome was obesity, defined as a weight-for-height z-score >3. The secondary outcomes included self-reported pneumonia, anemia defined as hemoglobin <110 g/L, and neurobehavioral disorders identified by Child Behavior Checklist and Bayley Scales of Infant Development. Compared with SVD, CDMR was associated with increased risks of obesity (adjusted odds ratio [aOR] 1.41; 95% confidence interval [CI] 1.14-1.75) and anemia (aOR 1.65; 95% CI 1.28-2.12), but not with the risk of pneumonia (aOR 1.16; 95% CI 0.94-1.45) or neurobehavioral disorders (aORs varied from 0.82 to 1.13) in childhood. Conclusion: CD, independent of cesarean indications, is likely associated with childhood obesity and anemia, indicating a need to keep pregnant women informed, especially those seeking CDMR, a need to explore possible improvement on obstetric service, and even a need for main stakeholders to reach a compromise in making a cesarean decision.
A Survey on SDN-based Intrusion Detection Systems on the Internet of Thing: Concepts, Issues, and Blockchain Applications
Authors: Hassan Heba A. , Hemdan Ezz E. , El-Shafai Walid , Shokair Mona , El-Samie Fathi E. Abd
Published: July 2021

With the accelerated development of computer networks utilization and the enormous growth of the number of applications running on top of it, network security becomes more significant. Intrusion Detection Systems (IDS) is considered as one of the essential tools utilized to protect computer networks and information systems. Software-defined network (SDN) architecture is used to provide network monitoring and analysis mechanism due to the programming environment of the SDN controller. On the other hand intrusion detection system is developed to monitor incoming traffic to the SDN network; hence it enables SDN to adjust security service insertion. This paper presents a survey study for SDN with the Internet of Things (IoT) and its improved versions like SDN-based IDS and SDN-based IoT. Likewise, discussing the IoT and its problems, especially the security aspects and solutions to overcome these problems. Finally, a brief description of the Blockchain concept and how it can be merged with an SDN-based IoT system to further enhance its security aspects is provided.
Study of Antimicrobial Susceptibility And Biofilm Formation of Cronobacter Sakazakii Isolates From Neonatal Sepsis In Southwest Iran
Authors: Moradi Melika , dezfuli Aram asareh zdegan , Dargahi Zahra , Amin Mansour , Ahmadkhosravi Nazanin , Motahar Moloudsadat , Jahangirimehr Fatemeh , Meghdadi Hossein , Bakhtiyariniya Pezhman , Heydari Reza , Emam Nasrin , Mehdipour Golshan
Published: July 2021

The authors have requested that this preprint be removed from Research Square.
Association between dietary fatty acids pattern and incidence of oral cancer: A case-control study from Southeast China
Authors: Fan Yi , Qiu Yu , Chen Qing , Wang Sijie , Xu Mingming , Li Yanni , Weng Yanfeng , Qian Jiawen , Wang Jing , Chen Fa , Wang Jing , Shi Bin , Pan Lizhen , Lin Lisong , He Baochang , Liu Fengqiong
Published: July 2021

ObjectiveTo investigate the relationship between dietary fatty acid pattern and the risk of oral cancer (OC).MethodIn 446 patients with primary oral cancer and 448 controls, we assessed prediagnosis consumption of 159 food items by food frequency questionnaires completed within 1 week of diagnosis. Fatty acid patterns were identified using principal component analysis. Odds ratio (OR) and 95% confidence (CI) interval was calculated.ResultsGeneral differences of fatty acid intake were observed between case and control, intake of saturated fatty acids such as C14:0, C16:0, C18:0 are higher in case than control group (p < 0.001), intake of monounsaturated fatty acid such as C18:1 is higher in case than control group (p < 0.001). We identified a fatty acid pattern which was characterized by saturated fatty acids and it explained 33.2% of the overall variability of the 32 fatty acids. The identified fatty acid pattern scores were positively associated with a higher risk of oral cancer [multivariable-adjusted OR comparing extreme quintiles, 3.325 (95%CI: 2.222, 4.975); Ptrend<0.001].ConclusionsGeneral differences of dietary fatty acids were observed between oral cancer patients and controls. A dietary fatty acids pattern, which was mainly composed of saturated fatty acids were positively correlated with the risk of oral cancer.
Diet Content of Non-Saturated Fatty Acids is Related to Lower Plasma Adiponectin in Patients with Metabolic Syndrome
Authors: Suárez-Cuenca Juan Antonio , Díaz-Jiménez Diana Elisa , Pineda-Juárez Juan Antonio , Mendoza-Mota Alondra Gissel , Valencia-Aldana Ofelia Dinora , Núñez-Angeles Said , Vera-Gómez Eduardo , Hernández-Patricio Alejandro , Escamilla-Tilch Mónica , Loeza-Magaña Pavel , Lara-Vargas Jorge Antonio , de Vaca Rebeca Pérez-Cabeza , Arteaga-Martínez José Rodolfo , Garduño-Pérez Ángel Alfonso , Barranco-Fragoso Beatriz , Montoya-Ramírez Jesús , Díaz-Aranda María Angélica , Chaparro-Hernández Roberto Carlos , Melchor-López Alberto , García Silvia , Gutiérrez-Salinas José , Mondragón-Terán Paul
Published: July 2021

Introduction. The metabolic syndrome (MS) has been related to the unbalance between pro- and anti-inflammatory cytokines; where adiponectin, an anti-inflammatory adipokine, is considered to play a key metabolic role. The consumption of certain micronutrients has been claimed to modify pro- and anti-inflammatory cytokines. Aim. To explore whether dietary micronutrients are related with plasma adiponectin in patients with MS. Methods. Cross-sectional analysis. Quartiles of dietary bioactive compounds were compared according to values of plasma adiponectin. Interquartile variation (IQV) and correlation analyses were performed. Results. There was a significant IQV of dietary unsaturated fatty acids (between -50% to -66% of change, p25 vs p75, p<0.05), particularly for trans-fatty acids, poly-unsaturated fatty acids, mono-unsaturated fatty acids and w-6 fatty acids, in relation to plasma adiponectin; as well as a negative correlation (rho= -0.53, -0.37, -0.29 and -0.34, respectively; p<0.05). Conclusion. Dietary amounts of unsaturated fatty acids inversely related to plasma adiponectin in patients with MS.