bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating Poor Sleep Quality and Associated Factors During the COVID-19 Pandemic: A Population-Based Survey in Bangladesh
Authors: Islam Md. Saiful , Rahman Md. Estiar , Zubayer Abdullah Al , Bhuiyan Md. Rifat Al Mazid , Khan Md. Kamrul Ahsan , Hossain Liakat , Sujon Md. Monjurul Ahasan
Published: November 2021

Background: The coronavirus disease 2019 (COVID-19) pandemic has adversely affected the sleep quality of individuals, and is a poorly investigated area. This study aimed to investigate the prevalence estimate of poor sleep quality and its associated factors among Bangladeshi residents during the COVID-19 pandemic.

Methods: An online cross-sectional survey was carried out from July 20 to August 5, 2020, involving 975 Bangladeshi residents (male: 54.2%; mean age: 26.7 ± 9.4 years; age range: 18–75 years). A self-reported questionnaire was answered by the respondents, covering information on demographic characteristics, perceived physical health status, COVID-19-related factors, COVID-19-induced anxiety assessment, and sleep quality. To assess sleep quality, the Bangla version of the Pittsburgh Sleep Quality Index was used. Logistic regression models were performed to analyze the factors associated with sleep quality.

Results: The prevalence estimate of poor sleep quality was 55.1% among the Bangladeshi people during the COVID-19 pandemic. As per the multiple regression analysis, poor sleep quality was significantly higher among respondents who reported female gender, moderate/poor health status, indirect contact with COVID-19 infected patients, decreased household income due to the COVID-19 pandemic, fear of infection, and COVID-19-induced anxiety.

Conclusion: Poor sleep quality was slightly prevalent among general people in Bangladesh during the COVID-19 pandemic. The findings indicate an immediate response for this vulnerable group to improve the sleep quality during the public health emergency of COVID-19.
Frailty in Indigenous Populations: A Scoping Review
Authors: Lewis Ebony T. , Howard Leanne , Cardona Magnolia , Radford Kylie , Withall Adrienne , Howie Adam , Rockwood Kenneth , Peters Ruth
Published: November 2021

Background: Indigenous populations experience high rates of age-related illness when compared to their non-Indigenous counterparts. Frailty is a challenging expression of aging and an important public health priority. The purpose of this review was to map what the existing literature reports around frailty in Indigenous populations and to highlight the current gaps in frailty research within the Indigenous landscape.

Method: Scoping review of English language original research articles focusing on frailty within Indigenous adult populations in settler colonial countries (Australia, Canada, New Zealand and USA). Ten electronic databases and eight relevant institutional websites were searched from inception to October 2020.

Results: Nine articles met our inclusion criteria, finding this population having a higher prevalence of frailty and frailty occurring at younger ages when compared to their non-Indigenous counterparts, but two did not use a formal frailty tool. Females presented with higher levels of frailty. No culturally specific frailty tool was identified, and the included articles did not assess strategies or interventions to manage or prevent frailty in Indigenous peoples.

Conclusions: There was little definitive evidence of the true frailty prevalence, approaches to frailty screening and of potential points of intervention to manage or prevent the onset of frailty. Improvements in the quality of evidence are urgently needed, along with further research to determine the factors contributing to higher rates of frailty within Indigenous populations. Incorporation of Indigenous views of frailty, and instruments and programs that are led and designed by Indigenous communities, are crucial to address this public health priority.
Export-Driven, Extensive Coastal Aquaculture Can Benefit Nutritionally Vulnerable People
Authors: Mamun Abdullah-Al , Murray Francis J. , Sprague Matthew , McAdam Bruce J. , Roos Nanna , de Roos Baukje , Pounds Alexandra , Little David C.
Published: November 2021

Export-orientated shrimp and prawn farming in coastal ghers has been associated with negative environmental, social, and nutritional impacts. This study challenges these perceptions based on field observations from four communities in South West Bangladesh. Most households observed (>60%) were either directly involved in seafood farming or engaged elsewhere in the seafood value chain. Our study set out to establish how the type and location of aquaculture impacted on access to and consumption of aquatic animals. Additionally, we assessed the effects of both household socioeconomic status and intra-household food allocation on individual diet and nutritional outcomes. We used a blended approach, including a 24-h consumption recall on two occasions, analysis of the proximate composition of aquatic animals and biomarkers from whole blood from a sample of the target population. The diverse polyculture systems generated broad social benefits, where “export-oriented” production actually supplied more food locally than to global markets. Key findings: (1) worse-off households achieved higher productivity of farmed aquatic animals on smaller landholding than better-off households with larger landholdings; (2) vegetable production on gher dikes was a significant source of nutrition and income in lower saline gradients; (3) more fish was eaten in lower saline gradients although fish consumption was highly variable within and between households; (4) intra-household allocation of specific foods within diets were similar across communities; (5) recommended nutrient intakes of protein and zinc exceeded daily requirements for adolescent females, but energy, calcium, and iron were below recommended intake levels; (6) n-3 LC-PUFA, expressed as percentage of total fatty acids, in whole blood samples of adolescent females declined with ambient salinity level regardless of household socioeconomic status; (7) analysis of aquatic animals consumed found that mangrove species and tilapia harvested from higher saline ghers contained high levels of desirable PUFAs. These findings suggest that export-driven, extensive coastal aquaculture can be nutrition sensitive when co-products are retained for local consumption.
Effects of FecB Mutation on Estrus, Ovulation, and Endocrine Characteristics in Small Tail Han Sheep
Authors: Wang Xiangyu , Guo Xiaofei , He Xiaoyun , Liu Qiuyue , Di Ran , Hu Wenping , Cao Xiaohan , Zhang Xiaosheng , Zhang Jinlong , Chu Mingxing
Published: November 2021

The Booroola fecundity gene (FecB) has a mutation that was found to increase the ovulation rate and litter size in Booroola Merino sheep. This mutation is also associated with the fecundity of small-tail han (STH) sheep, an important maternal breed used to produce hybrid offspring for mutton production in China. Previous research showed that the FecB gene affects reproduction in STH sheep, based on litter size records. However, the effects of this gene on estrus, ovulation, and endocrine characteristics in these sheep remain unclear. Here, we analyzed the traits mentioned earlier and compared them among the three FecB genotypes of STH ewes using estrus synchronization. Overall, 53 pluriparous ewes were selected from among 890 STH ewes and subjected to FecB genotyping for experiments to characterize estrous and ovulation rates. FecB heterozygous (+B) ewes presented an earlier onset of estrus (42.9 ± 2.2 h) and a shorter estrous cycle (17.2 ± 0.2 days) (P ≤ 0.05). The ovulation rates increased with the increasing copy number of the B allele (P ≤ 0.01). Ovulation time showed no significant differences among the three FecB genotypes. The serum concentrations of follicle-stimulating hormone (FSH), luteinizing hormone, estrogen (E2), and progesterone (P4) were measured in 19 of the ewes. Serum concentrations of E2 and FSH dramatically varied around the time of behavioral estrus. In FecB mutant homozygous (BB) ewes, E2 concentration had two peaks, which were higher (P ≤ 0.05) than those of ++ genotypes. FSH concentration of BB ewes was higher (P ≤ 0.05) than that of the ++ ewes just after estrus. The expression of the estrogen receptor 1 (ESR1) gene in the +B genotype was higher than in the other genotypes. Based on the data for the reproductive performance of STH ewes with the three FecB genotypes, our study suggests that the development of follicles in ewes with the B allele is dependent on the response to FSH regulated by E2 in the early stage. +B ewes, exhibiting moderate ovulation and litter size and a shorter estrous cycle, can be highly recommended in sheep crossbreeding systems for commercial mutton production. Moreover, this study provides useful information to conserve better and use the genetic resources of STH sheep in China.
Predefined-Time Control of Full-Scale 4D Model of Permanent-Magnet Synchronous Motor with Deterministic Disturbances and Stochastic Noises
Authors: de la Cruz Nain , Basin Michael
Published: November 2021

This paper presents a predefined-time convergent robust control algorithm that allows the control designer to set the convergence time in advance, independently of initial conditions, deterministic disturbances, and stochastic noises. The control law is consequently designed and verified by simulations for a full-scale 4-degrees-of-freedom (4D) permanent-magnet synchronous motor (PMSM) system in cases of a disturbance-free system with completely measurable states, a disturbance-free system with incompletely measurable states, a system with incompletely measurable states in the presence of deterministic disturbances, and a system with incompletely measurable states in the presence of both deterministic disturbances and stochastic noises. Numerical simulations are provided for the full-scale 4D PMSM system in order to validate the obtained theoretical results in each of the considered cases. To the best of our knowledge, this is the first attempt to design a predefined-time convergent control law for multi-dimensional systems with incompletely measurable states in the presence of both deterministic disturbances and stochastic noises.
SMART4Pain: Feasibility of a Two-Arm Pilot Study of an Integrated Rehabilitation Program for Adolescents and Their Parents for Improving Pain Management
Authors: Failo Alessandro , Venuti Paola , Villotti Patrizia , Schmalholz Stefan , Chistè Nicola , Raffeiner Bernd , Salandin Michela , Pellegrin Serena , Pescollderungg Lydia , Mazzoldi Mariantonietta
Published: November 2021

Chronic pain in youth has an unsung etiology and limited treatment options. Affected adolescents show difficulties in different functioning domains, and their parents can develop associated distress, which negatively influences the adolescent’s capacity to adjust to pain. The aims of this study are the following: (1) to develop an internet-delivered (online) pain intervention (SMART4Pain) program for adolescents and their parents, and to test its feasibility and acceptability; (2) to evaluate, in adolescents, the impact of the face-to-face, randomized, two-armed (i.e., CBT or biofeedback), open-label pilot study, developed together with the online program. The overall program consisted of six sessions scheduled over six weeks. Twenty adolescents (N = 20) and their parents (N = 20) completed the entire program and are included in this study. The results showed that all interventions were feasible and acceptable, as well as potentially effective in improving quality of life. Only the group receiving the biofeedback intervention showed some improvements in psychological indicators of stress. In conclusion, more research is needed to better understand and develop new, multimodal rehabilitation programs in outpatient settings.
Estimation and Separation of Longitudinal Dynamic Stability Derivatives with Forced Oscillation Method Using Computational Fluid Dynamics
Authors: Juliawan Nadhie , Chung Hyoung-Seog , Lee Jae-Woo , Kim Sangho
Published: November 2021

This paper focuses on estimating dynamic stability derivatives using a computational fluid dynamics (CFD)-based force oscillation method, and on separating the coupled dynamic derivatives terms obtained from the method. A transient RANS solver is used to calculate the time history of aerodynamic moments for a test model oscillating about the center of gravity, from which the coupled dynamic derivatives are estimated. The separation of the coupled derivatives term is carried out by simulating simple harmonic oscillation motions such as plunging motion and flapping motion which can isolate the pitching moment due to AOA rate (Cmα˙) and the pitching moment due to pitch rate (Cmq), respectively. The periodic motions are implemented using a CFD dynamic mesh technique with user-defined function (UDF). For the validation test, steady and unsteady simulations are performed on the Army-Navy Finner Missile model. The static aerodynamic moments and pressure distribution, as well as the coupled dynamic derivative results from the pitching oscillation mode, show good agreement with the previously published wind tunnel tests and CFD analysis data. In order to separate the coupled derivative terms, two additional harmonic oscillation modes of plunging and flapping motions are tested with the angle of attack variations from 0 to 85 degrees at a supersonic speed to provide real insight on the missile maneuverability. The cross-validation study between the three oscillation modes indicates the summation of the individual plunging and flapping results becoming nearly identical to the coupled derivative results from the pitching motion, which implies the entire set of coupled and separated dynamic derivative terms can be effectively estimated with only two out of three modes. The advantages and disadvantages of each method are discussed to determine the efficient approach of estimating the dynamic stability derivatives using the forced oscillation method.
Development of a 6-DOF Testing Platform for Multirotor Flying Vehicles with Suspended Loads
Authors: Mukras Saad M. S. , Omar Hanafy M.
Published: November 2021

The development of multirotor vehicles can often be a dangerous and costly undertaking due to the possibility of crashes resulting from faulty controllers. The matter of safety in such activities has primarily been addressed through the use of testbeds. However, testbeds for testing multirotor vehicles with suspended loads have previously not been reported. In this study, a simple yet novel testing platform was designed and built to aid in testing and evaluating the performances of multirotor flying vehicles, including vehicles with suspended loads. The platform allows the flying vehicle to move with all six degrees of freedom (DOF). Single or three-DOF motions can also be performed. Moreover, the platform was designed to enable the determination of the mass properties (center of mass and moments of inertia) of small multirotor vehicles (which are usually required in the development of new control systems). The applicability of the test platform for the in-flight performance testing of a multirotor vehicle was successfully demonstrated using a Holybro X500 quadcopter with a suspended load. The test platform was also successfully used to determine the mass properties of the vehicle.
Exercise Cuts Both Ways with ROS in Remodifying Innate and Adaptive Responses: Rewiring the Redox Mechanism of the Immune System during Exercise
Authors: Thirupathi Anand , Gu Yaodong , Pinho Ricardo Aurino
Published: November 2021

Nearly all cellular functions depend on redox reactions, including those of immune cells. However, how redox reactions are rearranged to induce an immune response to the entry of pathogens into the host is a complex process. Understanding this scenario will facilitate identification of the roles of specific types of reactive oxygen species (ROS) in the immune system. Although the detrimental effect of ROS could support the innate immune system, the adaptive immune system also requires a low level of ROS in order to stimulate various molecular functions. The requirements and functions of ROS vary in different cells, including immune cells. Thus, it is difficult to understand the specific ROS types and their targeting functions. Incomplete transfer of electrons to a specific target, along with failure of the antioxidant response, could result in oxidative-damage-related diseases, and oxidative damage is a common phenomenon in most immune disorders. Exercise is a noninvasive means of regulating ROS levels and antioxidant responses. Several studies have shown that exercise alone boosts immune functions independent of redox reactions. Here, we summarize how ROS target various signaling pathways of the immune system and its functions, along with the possible role of exercise in interfering with immune system signaling.
Analyzing the Neutron Parameters in the Accelerator Driven Subcritical Reactor Using the Mixture of Molten Pb-Bi as Both Target and Coolant
Authors: Tran Minh Tien
Published: November 2021

In this paper, the Accelerator Driven Subcritical Reactor (ADSR) was simulated based on the structure of the TRIGA-Mark II reactor by the MCNPX program. The proton beam interacts on the Pb-Bi molten target with various energy levels from 0.5 GeV to 2.0 GeV. The important neutron parameters to evaluate the operability of ADSR were calculated as: the neutron yields according to various thicknesses of the target and according to the energy of the incident proton beam; the effective neutron multiplication factor for various fuel mixtures, along with its stability for some fuel mixtures; the axial and radial distributions of the neutron flux along with the height and radius of the core. The obtained results had shown a good agreement in using Pb-Bi molten as the interaction target and coolant for ADSR.
Special delEVery: Extracellular Vesicles as Promising Delivery Platform to the Brain
Authors: Pauwels Marie J. , Vandendriessche Charysse , Vandenbroucke Roosmarijn E.
Published: November 2021

The treatment of central nervous system (CNS) pathologies is severely hampered by the presence of tightly regulated CNS barriers that restrict drug delivery to the brain. An increasing amount of data suggests that extracellular vesicles (EVs), i.e., membrane derived vesicles that inherently protect and transfer biological cargoes between cells, naturally cross the CNS barriers. Moreover, EVs can be engineered with targeting ligands to obtain enriched tissue targeting and delivery capacities. In this review, we provide a detailed overview of the literature describing a natural and engineered CNS targeting and therapeutic efficiency of different cell type derived EVs. Hereby, we specifically focus on peripheral administration routes in a broad range of CNS diseases. Furthermore, we underline the potential of research aimed at elucidating the vesicular transport mechanisms across the different CNS barriers. Finally, we elaborate on the practical considerations towards the application of EVs as a brain drug delivery system.
VR and AR Restoration of Urban Heritage: A Virtual Platform Mediating Disagreement from Spatial Conflicts in Korea
Authors: Youn Hyun-Chul , Ryoo Seong-Lyong
Published: November 2021

This study sought to uncover (1) the disagreement of spatial conflict between urban heritage and surrounding urban structure using two case studies from Korea—the main gate of the royal palace (Gwanghwamun) and the urban park containing celebrity graves (Hyoch’ang Park)—and (2) whether digital heritage restoration may mediate spatial conflict. A historical literature review and field surveys were conducted, with three main findings. First, the place identity of Gwanghwamun and Hyoch’ang Park, rooted in the Josŏn Dynasty, was seriously damaged during the Japanese colonial period. Although there were national attempts to recover the place identities of these sites during the modern period, limitations existed. Second, the restoration of Gwanghwamun’s Wŏltae (podium) and the relocation of Ŭiyŏlsa (the shrine of Hyoch’ang Park), which involved spatial transformation based on heritage, emerged in conflict with their surrounding urban structures—we identify a spatial conflict between local residents and stakeholders’ memories and the histories of these sites. Third, Donŭimun (the west gate of the city wall of the Josŏn Dynasty) digital restoration is a case mediating the conflict by restoring a sense of place in a virtual space and activating the cultural memory of the public by showcasing properties.
Premade Nanoparticle Films for the Synthesis of Vertically Aligned Carbon Nanotubes
Authors: Hoque Abdul , Ullah Ahamed , Guiton Beth S. , Alvarez Noe T.
Published: November 2021

Carbon nanotubes (CNTs) offer unique properties that have the potential to address multiple issues in industry and material sciences. Although many synthesis methods have been developed, it remains difficult to control CNT characteristics. Here, with the goal of achieving such control, we report a bottom-up process for CNT synthesis in which monolayers of premade aluminum oxide (Al2O3) and iron oxide (Fe3O4) nanoparticles were anchored on a flat silicon oxide (SiO2) substrate. The nanoparticle dispersion and monolayer assembly of the oleic-acid-stabilized Al2O3 nanoparticles were achieved using 11-phosphonoundecanoic acid as a bifunctional linker, with the phosphonate group binding to the SiO2 substrate and the terminal carboxylate group binding to the nanoparticles. Subsequently, an Fe3O4 monolayer was formed over the Al2O3 layer using the same approach. The assembled Al2O3 and Fe3O4 nanoparticle monolayers acted as a catalyst support and catalyst, respectively, for the growth of vertically aligned CNTs. The CNTs were successfully synthesized using a conventional atmospheric pressure-chemical vapor deposition method with acetylene as the carbon precursor. Thus, these nanoparticle films provide a facile and inexpensive approach for producing homogenous CNTs.
Microtubule-Based Mitochondrial Dynamics as a Valuable Therapeutic Target in Cancer
Authors: Vona Rosa , Mileo Anna Maria , Matarrese Paola
Published: November 2021

Mitochondria constitute an ever-reorganizing dynamic network that plays a key role in several fundamental cellular functions, including the regulation of metabolism, energy production, calcium homeostasis, production of reactive oxygen species, and programmed cell death. Each of these activities can be found to be impaired in cancer cells. It has been reported that mitochondrial dynamics are actively involved in both tumorigenesis and metabolic plasticity, allowing cancer cells to adapt to unfavorable environmental conditions and, thus, contributing to tumor progression. The mitochondrial dynamics include fusion, fragmentation, intracellular trafficking responsible for redistributing the organelle within the cell, biogenesis, and mitophagy. Although the mitochondrial dynamics are driven by the cytoskeleton—particularly by the microtubules and the microtubule-associated motor proteins dynein and kinesin—the molecular mechanisms regulating these complex processes are not yet fully understood. More recently, an exchange of mitochondria between stromal and cancer cells has also been described. The advantage of mitochondrial transfer in tumor cells results in benefits to cell survival, proliferation, and spreading. Therefore, understanding the molecular mechanisms that regulate mitochondrial trafficking can potentially be important for identifying new molecular targets in cancer therapy to interfere specifically with tumor dissemination processes.
Vaccines as Priming Tools for T Cell Therapy for Epithelial Cancers
Authors: Kandalaft Lana E. , Harari Alexandre
Published: November 2021

Impressive progress has recently been made in the field of cancer immunotherapy with the adoptive transfer of T cells, a successful personalized strategy, and checkpoint inhibitors (CPI) having extended the survival of numerous patients. However, not all patients have been able to benefit from these innovations. A key determinant of the responsiveness to cancer immunotherapies is the presence of T cells within the tumors. These tumor-infiltrating lymphocytes (TILs) are crucial in controlling tumor growth and their activity is being potentiated by immunotherapies. Although some epithelial cancers are associated with spontaneous T-cell and B-cell responses, which makes them good candidates for immunotherapies, it remains to create strategies that would promote lymphocyte infiltration and enable sustained immune responses in immune-resistant tumors. Therapeutic cancer vaccines hold the potential of being able to render “cold”, poorly infiltrated tumors into “hot” tumors that would be receptive to cellular immunotherapies. In this review, we elaborate on the obstacles that need to be overcome and the strategies that are being explored to that end, including various types of antigen repertoires and different vaccine platforms and combinations with other available treatments.
A Comprehensive Prospective Comparison of Acute Skin Toxicity after Hypofractionated and Normofractionated Radiation Therapy in Breast Cancer
Authors: Borm Kai J. , Kleine Vennekate Johanne , Vagedes Jan , Islam Mohammad O. A. , Duma Marciana N. , Loos Maximilian , Combs Stephanie E. , Schiller Kilian , Klusen Sophie , Paepke Stefan , Kiechle Marion B. , Paepke Daniela
Published: November 2021

The current study aims to determine whether hypofractionated radiotherapy (HF) leads to lower rates of acute radiodermatitis compared to conventional normofractionated radiotherapy (CF). A total of 166 patients with invasive breast cancer or DCIS were included in a prospective cohort study. Evaluation of acute radiodermatitis was obtained before radiotherapy, at the end of the treatment (T1), and 6 weeks after the treatment (T2) using CTCAE (v5.0) scores, the Skindex-16 questionnaire, and ultrasound measurement of the skin. CTCAE and Skindex-16 scores in the CF-group were significantly higher compared to the HF group indicating more pronounced side effects at the end of the treatment (CTCAE: CF-RT 1.0 (IQR: 0.0) vs. HF-RT 0.0 (0.25); p = 0.03; Skindex-16: CF: 20.8 (IQR: 25.8); HF: 8.3 (27.1); p = 0.04). At 6 weeks after the treatment, no significant differences between the two fractionation schemes were observed. Ultrasound based assessment showed that the skin thickness in the treated breast was higher compared to the healthy breast at all time-points. However, no significant difference between HF and CF was seen either at T1 or T2. The current study complements and confirms pre-existing evidence that HF leads to a lower degree of acute radiodermatitis and better patient reported outcome compared to CF at the end of treatment. This should be considered whenever fractionation of adjuvant breast cancer treatment is being discussed.
Real-World Data from a Refractory Triple-Negative Breast Cancer Cohort Selected Using a Clinical Data Warehouse Approach
Authors: Kim Hana , Kim Hyo Jung , Kim Hongsik , Kim Hye Ryeon , Jo Hyunji , Hong Joohyun , Kim Ryul , Kim Ji-Yeon , Ahn Jin Seok , Im Young-Hyuck , Lee Se Kyung , Kim Haeyoung , Shin Soo-Yong , Park Yeon Hee
Published: November 2021

Purpose: Triple-negative breast cancer (TNBC) is well known for its aggressive course and poor prognosis. In this study, we sought to investigate clinical, demographic, and pathologic characteristics and treatment outcomes of patients with refractory, metastatic TNBC selected by a clinical data warehouse (CDW) approach. Patients and methods: Data were extracted from the real-time breast cancer registry integrated into the Data Analytics and Research Window for Integrated Knowledge C (DARWIN-C), the CDW of Samsung Medical Center. Between January 1997 and December 2019, a TNBC cohort was searched for in the breast cancer registry, which includes records from more than 40,000 patients. Among them, cases of pathologically confirmed metastatic TNBC (mTNBC) were selected as the cohort group (n = 451). The extracted data from the registry via the CDW platform included clinical, pathological, laboratory, and chemotherapy information. Refractory TNBC was defined as confirmed distant metastasis within one year after adjuvant treatment. Results: This study comprised a total of 451 patients with mTNBC, including 69 patients with de novo mTNBC, 131 patients in the nonrefractory TNBC group with confirmed stage IV disease after one year of adjuvant treatment, and 251 patients with refractory mTNBC, whose disease recurred as stage IV within one year after completing adjuvant treatment. The refractory mTNBC cohort was composed of patients with disease that recurred at stage IV after surgery (refractory mTNBC after surgery) (n = 207) and patients in whom metastasis was confirmed during neoadjuvant chemotherapy (unresectable TNBC due to progression during neoadjuvant chemotherapy) (n = 44). Patients in the refractory mTNBC group were younger than those in the nonrefractory group (median age 46 vs. 51 years; p < 0.001). Considering the pathological findings, the refractory group had a greater proportion of cases with Ki-67 ≥ 3+ than did the nonrefractory group (71% vs. 47%; p = 0.004). During a median 8.4 years of follow-up, the overall survival was 24.8 months in the nonrefractory mTNBC group and 14.3 months in the refractory mTNBC group (p < 0.001), and the median progression-free survival periods were 6.2 months and 4.2 months, respectively (p < 0.001). The median disease-free survival period was 30.1 months in the nonrefractory mTNBC group and only 7.6 months in the refractory mTNBC group. Factors related to metastatic sites affecting overall survival were liver metastasis at diagnosis (p < 0.001) and leptomeningeal involvement (p = 0.001). Conclusions: We revealed that patients with refractory mTNBC had a much poorer prognosis among all mTNBC cases and described the characteristics of this patient group.
The Role of Trastuzumab in Patients with HER2 Positive Small (pT1mi/a) Breast Cancers, a Multicenter Retrospective Study
Authors: Villasco Andrea , Actis Silvia , Bounous Valentina Elisabetta , Borella Fulvio , D’Alonzo Marta , Ponzone Riccardo , De Sanctis Corrado , Benedetto Chiara , Biglia Nicoletta
Published: November 2021

The treatment with adjuvant Trastuzumab in patients diagnosed with HER2+ small breast cancers is controversial: limited prospective data from randomized trials is available. This study aims to measure the effect of Trastuzumab in the early stages of breast cancer (pT1mic/a pN0/1mi) in terms of disease recurrence and to identify which are the factors that most affect the prognosis of small HER2+ tumors. One hundred HER2+ pT1mic-pT1a breast cancer patients who were treated in three Turin Breast Units between January 1998 and December 2018 were retrospectively selected and reviewed. Trastuzumab was administered to 23 patients. Clinicopathological features and disease-free survival (DFS) were compared between different subgroups. The primary outcome was the disease recurrence rate. Median follow-up time was 86 months. Compared to pT1a tumors, pT1mic lesions had a higher tumor grade (84% of pT1mic vs. 55% of pT1a; p = 0.003), a higher Ki-67 index (81% vs. 46%; p = 0.007) and were more frequently hormone receptor (HR) negative (69% vs. 36%, p = 0.001). Disease recurrence rate was significantly lower among patients who received adjuvant Trastuzumab (p = 0.02), with this therapy conferring an 85% reduction in the risk of relapse (HR 0.15; p = 0.02). Among the patients who did not receive adjuvant Trastuzumab, the only factor significantly associated with an increased risk of developing a recurrence was the immunohistochemical (IHC) subtype: in fact, HR− HER2+ tumors showed a risk seven times higher of relapsing (HR 7.29; p = 0.003). Adjuvant Trastuzumab appears to reduce the risk of disease recurrence even in small HER2+ tumors. The adjuvant targeted therapy should be considered in patients with HR− HER2+ tumors since they have the highest risk of recurrence, independently from size and grade.
Clinicopathological Determinants of Recurrence Risk and Survival in Mucinous Ovarian Carcinoma
Authors: Hollis Robert L. , Stillie Lorna J. , Hopkins Samantha , Bartos Clare , Churchman Michael , Rye Tzyvia , Nussey Fiona , Fegan Scott , Nirsimloo Rachel , Inman Gareth J. , Herrington C. Simon , Gourley Charlie
Published: November 2021

Mucinous ovarian carcinoma (MOC) is a unique form of ovarian cancer. MOC typically presents at early stage but demonstrates intrinsic chemoresistance; treatment of advanced-stage and relapsed disease is therefore challenging. We harness a large retrospective MOC cohort to identify factors associated with recurrence risk and survival. A total of 151 MOC patients were included. The 5 year disease-specific survival (DSS) was 84.5%. Risk of subsequent recurrence after a disease-free period of 2 and 5 years was low (8.3% and 5.6% over the next 10 years). The majority of cases were FIGO stage I (35.6% IA, 43.0% IC). Multivariable analysis identified stage and pathological grade as independently associated with DSS (p < 0.001 and p < 0.001). Grade 1 stage I patients represented the majority of cases (53.0%) and demonstrated exceptional survival (10 year DSS 95.3%); survival was comparable between grade I stage IA and stage IC patients, and between grade I stage IC patients who did and did not receive adjuvant chemotherapy. At 5 years following diagnosis, the proportion of grade 1, 2 and 3 patients remaining disease free was 89.5%, 74.9% and 41.7%; the corresponding proportions for FIGO stage I, II and III/IV patients were 91.1%, 76.7% and 19.8%. Median post-relapse survival was 5.0 months. Most MOC patients present with low-grade early-stage disease and are at low risk of recurrence. New treatment options are urgently needed to improve survival following relapse, which is associated with extremely poor prognosis.
Quantitative Analysis of Cell Aggregation Dynamics Identifies HDAC Inhibitors as Potential Regulators of Cancer Cell Clustering
Authors: Gava Fabien , Pignolet Julie , Déjean Sébastien , Mondésert Odile , Morin Renaud , Agossa Joseph , Ducommun Bernard , Lobjois Valérie
Published: November 2021

Characterization of the molecular mechanisms involved in tumor cell clustering could open the way to new therapeutic strategies. Towards this aim, we used an in vitro quantitative procedure to monitor the anchorage-independent cell aggregation kinetics in a panel of 25 cancer cell lines. The analysis of the relationship between selected aggregation dynamic parameters and the gene expression data for these cell lines from the CCLE database allowed identifying genes with expression significantly associated with aggregation parameter variations. Comparison of these transcripts with the perturbagen signatures from the Connectivity Map resource highlighted that they were strongly correlated with the transcriptional signature of most histone deacetylase (HDAC) inhibitors. Experimental evaluation of two HDAC inhibitors (SAHA and ISOX) showed that they inhibited the initial step of in vitro tumor cell aggregation. This validates our findings and reinforces the potential interest of HDCA inhibitors to prevent metastasis spreading.
Surveillance for Patients with Oral Squamous Cell Carcinoma after Complete Surgical Resection as Primary Treatment: A Single-Center Retrospective Cohort Study
Authors: Fukumoto Chonji , Oshima Ryo , Sawatani Yuta , Shiraishi Ryo , Hyodo Toshiki , Kamimura Ryouta , Hasegawa Tomonori , Komiyama Yuske , Izumi Sayaka , Fujita Atsushi , Wakui Takahiro , Kawamata Hitoshi
Published: November 2021

Background: The surveillance methods oral squamous cell carcinoma (OSCC) patients may be chosen by considering the risk for recurrence, and it is important to establish appropriate methods during the period in which latent/dormant cancer cells become more apparent. To investigate the appropriate surveillance of patients with OSCC based on the individual risk for recurrence and/or metastasis, we performed a retrospective cohort study after the complete surgical resection of OSCC as the primary treatment. Methods: The study was performed in 324 patients with OSCC who had been primarily treated with surgery from 2007 to 2020 at our hospital. We investigated the period, timing, and methods (visual examination, palpation and imaging using FDG-PET/CT or CECT) for surveillance in each case that comprised postsurgical treatment. Results: Regarding the time to occurrence of postsurgical events, we found that half of cases of local recurrence, cervical lymph node metastasis, and distant metastasis occurred within 200 days, and 75% of all of these events occurred within 400 days. However, the mean time for second primary cancer was 1589 days. The postsurgical events were detected earlier by imaging examinations than they were by visual examination and palpation. Conclusions: For the surveillance of patients with OSCC after primary surgery, it is desirable to perform FDG-PET/CT within 3–6 months and at 1 year after surgery and to consider CECT as an option in between FDG-PET/CT, while continuing history and physical examinations for about 5 years based on individual risk assessment.
The Magnetic Response of Starphenes
Authors: Orozco-Ic Mesías , Merino Gabriel
Published: November 2021

The aromaticity of [n]starphenes (n = 1, 4, 7, 10, 13, 16), as well as starphene-based [19]dendriphene, is addressed by calculating the magnetically induced current density and the induced magnetic field, using the pseudo-π model. When an external magnetic field is applied, these systems create diatropic currents that split into a global peripheral current surrounding the starphene skeleton and several local currents in the acene-based arms, resulting in large shielding cones above the arms. In particular, the arm currents are smaller than their linear analogs, and in general, the strengths of the ring currents tend to weaken as the starphene get larger.
Plasmonic Biosensors for the Detection of Lung Cancer Biomarkers: A Review
Authors: Usman Fahad , Dennis John Ojur , Aljameel A.I. , Ali M.K.M. , Aldaghri O. , Ibnaouf K.H. , Zango Zakariyya Uba , Beygisangchin Mahnoush , Alsadig Ahmed , Meriaudeau Fabrice
Published: November 2021

Lung cancer is the most common and deadliest cancer type globally. Its early diagnosis can guarantee a five-year survival rate. Unfortunately, application of the available diagnosis methods such as computed tomography, chest radiograph, magnetic resonance imaging (MRI), ultrasound, low-dose CT scan, bone scans, positron emission tomography (PET), and biopsy is hindered due to one or more problems, such as phenotypic properties of tumours that prevent early detection, invasiveness, expensiveness, and time consumption. Detection of lung cancer biomarkers using a biosensor is reported to solve the problems. Among biosensors, optical biosensors attract greater attention due to being ultra-sensitive, free from electromagnetic interference, capable of wide dynamic range detection, free from the requirement of a reference electrode, free from electrical hazards, highly stable, capable of multiplexing detection, and having the potential for more information content than electrical transducers. Inspired by promising features of plasmonic sensors, including surface plasmon resonance (SPR), localised surface plasmon resonance (LSPR), and surface enhanced Raman scattering (SERS) such as ultra-sensitivity, single particle/molecular level detection capability, multiplexing capability, photostability, real-time measurement, label-free measurement, room temperature operation, naked-eye readability, and the ease of miniaturisation without sophisticated sensor chip fabrication and instrumentation, numerous plasmonic sensors for the detection of lung cancer biomarkers have been investigated. In this review, the principle plasmonic sensor is explained. In addition, novel strategies and modifications adopted for the detection of lung cancer biomarkers such as miRNA, carcinoembryonic antigen (CEA), cytokeratins, and volatile organic compounds (VOCs) using plasmonic sensors are also reported. Furthermore, the challenges and prospects of the plasmonic biosensors for the detection of lung cancer biomarkers are highlighted.
Interventions for Parental Anxiety in Preparation for Pediatric Surgery: A Narrative Review
Authors: Santapuram Pooja , Stone Amanda L. , Walden Rachel Lane , Alexander Louise
Published: November 2021

The preoperative experience can cause significant anxiety for both pediatric patients and their parents in the lead up to a surgical procedure. Pediatric anxiety in a preoperative setting has been shown to have significant negative downstream effects on the clinical outcomes of children and the healthcare system as a whole. Studies have found that preoperative parental anxiety has significant negative effects on children, regarding anxiety and emotional response. Therefore, interventions for parental preoperative anxiety are important to reduce the child’s anxiety. This review provides a brief overview of a broad range of strategies used to alleviate parental anxiety in a preoperative setting. Preoperative education, play-based interventions, music therapy, the presence of parents at induction of anesthesia, and integrative preoperative preparation programs have all demonstrated some evidence for reducing parental preoperative anxiety. The ultimate goal of using interventions for parental preoperative anxiety is to equip healthcare systems to better support families and optimize the perioperative outcomes of children.
Decreased Incidence of Pediatric Intussusception during COVID-19
Authors: Park Jun Sung , Byun Young-Hoon , Choi Seung Jun , Lee Jong Seung , Ryu Jeong-Min , Lee Jeong-Yong
Published: November 2021

Coronavirus disease 2019 (COVID-19) changed the epidemiology of various diseases. The present study retrospectively investigates the epidemiologic and clinical changes in pediatric intussusception for ages ≤ 7 years before (February 2019–January 2020) and after (February 2020–January 2021) the COVID-19 outbreak in a single pediatric emergency department of a university-affiliated tertiary hospital. The incidence of communicable diseases—defined as infectious diseases with the potential for human-to-human transmission via all methods, non-communicable diseases, and intussusception were decreased following the COVID-19 outbreak (15,932 to 3880 (24.4%), 12,994 to 8050 (62.0%), and 87 to 27 (31.0%), respectively). The incidence of intussusception correlated significantly with the change in incidence of communicable diseases (Poisson log-linear regression, odds ratio = 2.15, 95% CI = 1.08–4.26, and p = 0.029). Compared with the pre-pandemic period, patients of the pandemic period showed higher proportions of pathologic leading point (PLP) and hospitalization (14.8% vs. 2.3% and 18.5% vs. 4.6%, respectively), lower base excesses (−4.8 mmol/L vs. −3.6 mmol/L), and higher lactate concentrations (1.7 mmol/L vs. 1.5 mmol/L). The incidence of pediatric intussusception decreased after the COVID-19 pandemic. This reduced incidence may be related to the reduced incidence of communicable diseases. However, the proportions of more severe diseases and PLPs were higher after the COVID-19 pandemic.
Community-Level Impacts of Climate-Smart Agriculture Interventions on Food Security and Dietary Diversity in Climate-Smart Villages in Myanmar
Authors: Hanley Andrew , Brychkova Galina , Barbon Wilson John , Noe Su Myat , Myae Chan , Thant Phyu Sin , McKeown Peter C. , Gonsalves Julian , Spillane Charles
Published: November 2021

Diversification of production to strengthen resilience is a key tenet of climate-smart agriculture (CSA), which can help to address the complex vulnerabilities of agriculture-dependent rural communities. In this study, we investigated the relationship between the promotion of different CSA practices across four climate-smart villages (CSVs) in Myanmar. To determine the impact of the CSA practices on livelihoods and health, survey data were collected from agricultural households (n = 527) over three years. Within the time period studied, the results indicate that some the CSA practices and technologies adopted were significantly associated with changes in household dietary diversity scores (HDDS), but, in the short-term, these were not associated with improvements in the households’ food insecurity scores (HFIAS). Based on the survey responses, we examined how pathways of CSA practice adoption tailored to different contexts of Myanmar’s four agroecologies could contribute to the observed changes, including possible resulting trade-offs. We highlight that understanding the impacts of CSA adoption on household food security in CSVs will require longer-term monitoring, as most CSA options are medium- to long-cycle interventions. Our further analysis of knowledge, attitudes and practices (KAPs) amongst the households indicated a poor understanding of the household knowledge, attitudes and practices in relation to nutrition, food choices, food preparation, sanitation and hygiene. Our KAP findings indicate that current nutrition education interventions in the Myanmar CSVs are inadequate and will need further improvement for health and nutrition outcomes from the portfolio of CSA interventions.
Bacteria Cell Hydrophobicity and Interfacial Properties Relationships: A New MEOR Approach
Authors: Ganji-Azad Ehsan , Javadi Aliyar , Jahanbani Veshareh Moein , Ayatollahi Shahab , Miller Reinhard
Published: November 2021

For microbial enhanced oil recovery (MEOR), different mechanisms have been introduced. In some of these papers, the phenomena and mechanisms related to biosurfactants produced by certain microorganisms were discussed, while others studied the direct impacts of the properties of microorganisms on the related mechanisms. However, there are only very few papers dealing with the direct impacts of microorganisms on interfacial properties. In the present work, the interfacial properties of three bacteria MJ02 (Bacillus Subtilis type), MJ03 (Pseudomonas Aeruginosa type), and RAG1 (Acinetobacter Calcoaceticus type) with the hydrophobicity factors 2, 34, and 79% were studied, along with their direct impact on the water/heptane interfacial tension (IFT), dilational interfacial visco-elasticity, and emulsion stability. A relationship between the adsorption dynamics and IFT reduction with the hydrophobicity of the bacteria cells is found. The cells with highest hydrophobicity (79%) exhibit a very fast dynamic of adsorption and lead to relatively large interfacial elasticity values at short adsorption time. The maximum elasticity values (at the studied frequencies) are observed for bacteria cells with the intermediate hydrophobicity factor (34%); however, at longer adsorption times. The emulsification studies show that among the three bacteria, just RAG1 provides a good capability to stabilize crude oil in brine emulsions, which correlates with the observed fast dynamics of adsorption and high elasticity values at short times. The salinity of the aqueous phase is also discussed as an important factor for the emulsion formation and stabilization.
Systematics and Biogeography of the New World Genus Plumolepilius (Coleoptera: Curculionidae)
Authors: Barrios-Izás Manuel A. , Morrone Juan J.
Published: November 2021

Plumolepilius Barrios-Izás & Anderson, 2016 is a leaf litter weevil genus that inhabits montane broadleaf forests from southern Mexico to northern Panama. The genus consists of 27 species, 22 distributed in the Chiapas Highlands province (Mexican Transition Zone) and 5 found in the Pacific dominion (Neotropical region) in Costa Rica and Panamá. Here, we analyze the phylogenetic relationships of the species of Plumolepilius based on 20 external body characters and 9 characters from the genitalia. The first dichotomy of the cladogram separates two species from the Pacific dominion from the remaining species of Plumolepilius from the Chiapas Highlands province and three species restricted to the Pacific dominion. We hypothesize that redundant distributions in the taxon-area cladogram of the genus may be due to dispersal events, probably during the Pleistocene glaciations.