bluuebunny/crossref_metadata_2025 huggingface dataset:
The sex-specific metabolic signature of C57BL/6NRj mice during aging
Authors: Bresilla Doruntina , Habisch Hansjoerg , Pritišanac Iva , Zarse Kim , Parichatikanond Warisara , Ristow Michael , Madl Tobias , Madreiter-Sokolowski Corina T.
Published: December 2022

Due to intact reactive oxygen species homeostasis and glucose metabolism, C57BL/6NRj mice are especially suitable to study cellular alterations in metabolism. We applied Nuclear Magnetic resonance spectroscopy to analyze five different tissues of this mouse strain during aging and included female and male mice aged 3, 6, 12, and 24 months. Metabolite signatures allowed separation between the age groups in all tissues, and we identified the most prominently changing metabolites in female and male tissues. A refined analysis of individual metabolite levels during aging revealed an early onset of age-related changes at 6 months, sex-specific differences in the liver, and a biphasic pattern for various metabolites in the brain, heart, liver, and lung. In contrast, a linear decrease of amino acids was apparent in muscle tissues. Based on these results, we assume that age-related metabolic alterations happen at a comparably early aging state and are potentially associated with a metabolic switch. Moreover, identified differences between female and male tissues stress the importance of distinguishing between sexes when studying age-related changes and developing new treatment approaches. Besides, metabolomic features seem to be highly dependent on the genetic background of mouse strains.
1H NMR metabolomics analysis of oil palm stem tissue infected by Ganoderma boninense based on field severity Indices
Authors: Pancoro Adi , Karima Elfina , Apriyanto Ardha , Effendi Yunus
Published: December 2022

Basal stem rot disease (BSR) caused by G. boninense affects most oil palm plants in Southeast Asia. This disease can be fatal to palm oil production. BSR shows no signs on the tree in the early stages of infection. Therefore, it is essential to find an approach that can detect BSR disease in oil palm, especially at any level of disease severity in the field. This study aims to identify biomarkers of BSR disease in oil palm stem tissue based on various disease severity indices in the field using 1H NMR-based metabolomics analysis. The crude extract of oil palm stem tissue with four disease severity indices was analyzed by 1H NMR metabolomics. Approximately 90 metabolites from oil palm stem tissue were identified.Twenty of these were identified as metabolites that significantly differentiated the four disease severity indices. These metabolites include the organic acid group, the carbohydrate group, the organoheterocyclic compound group, and the benzoid group. In addition, different tentative biomarkers for different disease severity indices were also identified. These tentative biomarkers consist of groups of organic acids, carbohydrates, organoheterocyclic compounds, nitrogenous organic compounds, and benzene. There are five pathways in oil palm that are potentially affected by BSR disease.
A machine learning method for improving the accuracy of radiation biodosimetry by combining data from the dicentric chromosomes and micronucleus assays
Authors: Shuryak Igor , Royba Ekaterina , Repin Mikhail , Turner Helen C. , Garty Guy , Deoli Naresh , Brenner David J.
Published: December 2022

A large-scale malicious or accidental radiological event can expose vast numbers of people to ionizing radiation. The dicentric chromosome (DCA) and cytokinesis-block micronucleus (CBMN) assays are well-established biodosimetry methods for estimating individual absorbed doses after radiation exposure. Here we used machine learning (ML) to test the hypothesis that combining automated DCA and CBMN assays will improve dose reconstruction accuracy, compared with using either cytogenetic assay alone. We analyzed 1349 blood sample aliquots from 155 donors of different ages (3–69 years) and sexes (49.1% males), ex vivo irradiated with 0–8 Gy at dose rates from 0.08 Gy/day to ≥ 600 Gy/s. We compared the performances of several state-of-the-art ensemble ML methods and found that random forest generated the best results, with R2 for actual vs. reconstructed doses on a testing data subset = 0.845, and mean absolute error = 0.628 Gy. The most important predictor variables were CBMN and DCA frequencies, and age. Removing CBMN or DCA data from the model significantly increased squared errors on testing data (p-values 3.4 × 10–8 and 1.1 × 10–6, respectively). These findings demonstrate the promising potential of combining CBMN and DCA assay data to reconstruct radiation doses in realistic scenarios of heterogeneous populations exposed to a mass-casualty radiological event.
Lightweight cost-effective hybrid materials for energy absorption applications
Authors: El-baky Marwa A. Abd , Allah Mahmoud M. Awd , Kamel Madeha , Abd-Elaziem Walaa
Published: December 2022

The present paper experimentally explores the influence of the fiber hybridization and layering sequence on crashworthiness behavior and deformation history of polymer/metal thin-walled pipes. Jute (J)/glass (G) reinforced epoxy over wrapped aluminum (Al) pipes were prepared via hand wet wrapping then subjected to axial quasi-static compressive loads. The load versus displacement plots and crashing indicators, i.e. peak crushing load ($${\mathrm{F}}_{\mathrm{ip}}$$

F
ip

), mean crushing load ($${\mathrm{F}}_{\mathrm{m}}$$

F
m

), total energy absorption ($$\mathrm{U})$$

U
)

, specific energy absorption $$\left(\mathrm{SEA}\right)$$

SEA

, and crush force efficiency $$\left(\mathrm{CFE}\right)$$

CFE

were determined. Experimental results revealed that the maximum $$\left(\mathrm{SEA}\right)$$

SEA

was recorded for Al/2J/4G/2J pipe with a value of about 42.92 kJ/g, with an enhancement of 20.56% in $$\left(\mathrm{SEA}\right)$$

SEA

compared with pure Al-pipes. Al/2J/4G/2J specimens display the maximum ($$\mathrm{U})$$

U
)

, $$\left(\mathrm{SEA}\right)$$

SEA

, and $$\left(\mathrm{CFE}\right)$$

CFE

and could be employed as energy absorbing members in automobiles.
AS-CMC: a pan-cancer database of alternative splicing for molecular classification of cancer
Authors: Park Jiyeon , Lee Jin-Ok , Lee Minho , Chung Yeun-Jun
Published: December 2022

Alternative splicing (AS) is a post-transcriptional regulation that leads to the complexity of the transcriptome. Despite the growing importance of AS in cancer research, the role of AS has not been systematically studied, especially in understanding cancer molecular classification. Herein, we analyzed the molecular subtype-specific regulation of AS using The Cancer Genome Atlas data and constructed a web-based database, named Alternative Splicing for Cancer Molecular Classification (AS-CMC). Our system harbors three analysis modules for exploring subtype-specific AS events, evaluating their phenotype association, and performing pan-cancer comparison. The number of subtype-specific AS events was found to be diverse across cancer types, and some differentially regulated AS events were recurrently found in multiple cancer types. We analyzed a subtype-specific AS in exon 11 of mitogen-activated protein kinase kinase 7 (MAP3K7) as an example of a pan-cancer AS biomarker. This AS marker showed significant association with the survival of patients with stomach adenocarcinoma. Our analysis revealed AS as an important determinant for cancer molecular classification. AS-CMC is the first web-based resource that provides a comprehensive tool to explore the biological implications of AS events, facilitating the discovery of novel AS biomarkers.
Influencing factors and prediction methods of radiotherapy and chemotherapy in patients with lung cancer based on logistic regression analysis
Authors: Liu Yuxia , Xu Chang , Xing Chengyan , Chen Mingwei
Published: December 2022

Logistic regression analysis has widespread applications in clinical disease diagnosis, but it has not yet been applied to assess the acceptance of radiotherapy and chemotherapy in patients with lung cancer. A prediction model was established to investigate the influencing factors of radiotherapy and chemotherapy in lung cancer patients in order to provide useful information for clinicians to develop targeted and effective treatment. A sample was admitted of lung cancer patients to Binzhou Medical University Hospital stays from January 2020 to June 2021. After investigating doctors, nurses, patients, managers and conducting expert demonstration, the questionnaire was formed. The questionnaire was filled out by the patient or the patient's family members. The factors in the questionnaire data of patients accepting and not accepting radiotherapy and chemotherapy were compared for univariate analysis, and the significantly different single factor were analyzed by multifactor logistic regression analysis, explored the influencing factors of radiotherapy and chemotherapy in lung cancer patients established a predictive model and drew the receiver operating characteristic curve (ROC curve). The factors of two groups had statistically significant differences or no statistically significant differences. After multifactor logistic regression analysis was conducted, own personality, self-care ability, disease course classification, own attitude towards disease treatment, and family attitude towards disease treatment were included in the influencing factors of radiotherapy and chemotherapy in patients with lung cancer. Then, a predictive model was established. The area under the ROC curve of the predicted model was 0.973, the 95% confidence interval was 0.952–0.995, the optimal critical value was 0.832, the sensitivity was 91.84%, the specificity was 89.09%, and the accuracy was 90.85%. Based on logistic regression analysis, the prediction model could predict the extent of accepting radiotherapy and chemotherapy in patients with lung cancer. Understanding the factors related to patients with lung cancer accepting radiotherapy and chemotherapy could provide useful information for the targeted and effective treatment by clinicians.
Role of cardiac mitofusins in cardiac conduction following simulated ischemia–reperfusion
Authors: Kwek Xiu-Yi , Hall Andrew R. , Lim Wei-Wen , Katwadi Khairunnisa , Soong Poh Loong , Grishina Elina , Lin Kun-Han , Crespo-Avilan Gustavo , Yap En Ping , Ismail Nur Izzah , Chinda Kroekkiat , Chung Ying Ying , Wei Heming , Shim Winston , Montaigne David , Tinker Andrew , Ong Sang-Bing , Hausenloy Derek J.
Published: December 2022

Mitochondrial dysfunction induced by acute cardiac ischemia–reperfusion (IR), may increase susceptibility to arrhythmias by perturbing energetics, oxidative stress production and calcium homeostasis. Although changes in mitochondrial morphology are known to impact on mitochondrial function, their role in cardiac arrhythmogenesis is not known. To assess action potential duration (APD) in cardiomyocytes from the Mitofusins-1/2 (Mfn1/Mfn2)-double-knockout (Mfn-DKO) compared to wild-type (WT) mice, optical-electrophysiology was conducted. To measure conduction velocity (CV) in atrial and ventricular tissue from the Mfn-DKO and WT mice, at both baseline and following simulated acute IR, multi-electrode array (MEA) was employed. Intracellular localization of connexin-43 (Cx43) at baseline was evaluated by immunohistochemistry, while Cx-43 phosphorylation was assessed by Western-blotting. Mfn-DKO cardiomyocytes demonstrated an increased APD. At baseline, CV was significantly lower in the left ventricle of the Mfn-DKO mice. CV decreased with simulated-ischemia and returned to baseline levels during simulated-reperfusion in WT but not in atria of Mfn-DKO mice. Mfn-DKO hearts displayed increased Cx43 lateralization, although phosphorylation of Cx43 at Ser-368 did not differ. In summary, Mfn-DKO mice have increased APD and reduced CV at baseline and impaired alterations in CV following cardiac IR. These findings were associated with increased Cx43 lateralization, suggesting that the mitofusins may impact on post-MI cardiac-arrhythmogenesis.
Social dilemma in the excess use of antimicrobials incurring antimicrobial resistance
Authors: Ito Hiromu , Wada Takayuki , Ichinose Genki , Tanimoto Jun , Yoshimura Jin , Yamamoto Taro , Morita Satoru
Published: December 2022

The emergence of antimicrobial resistance (AMR) caused by the excess use of antimicrobials has come to be recognized as a global threat to public health. There is a ‘tragedy of the commons’ type social dilemma behind this excess use of antimicrobials, which should be recognized by all stakeholders. To address this global threat, we thus surveyed eight countries/areas to determine whether people recognize this dilemma and showed that although more than half of the population pays little, if any, attention to it, almost 20% recognize this social dilemma, and 15–30% of those have a positive attitude toward solving that dilemma. We suspect that increasing individual awareness of this social dilemma contributes to decreasing the frequency of AMR emergencies.
Broad host range may be a key to long-term persistence of bacteriophages infecting intestinal Bacteroidaceae species
Authors: Hedžet Stina , Rupnik Maja , Accetto Tomaž
Published: December 2022

The longitudinal studies have found that the human gut microbiota is stable over time with some major bacterial lineages or even strains persisting for years. This was recently extended to gut bacteriophages using the metagenomic data. Here, we focused on cultivation of the major Bacteroidetes of human gut, the Bacteroides and Phocaeicola strains, and their bacteriophages from two healthy donors. The persistence of Bacteroides and Phocaeicola species and strains was confirmed. We isolated 28 genetically different phages grouped into seven distinct clusters, two of these were new. Moreover, the bacteriophages from several groups, although being genetically quite homogeneous, had the ability to infect the strains belonging to different species isolated from several sampling time-points and different donors. We propose that the ability to infect several host species, which differ in their nutritional niches, may promote long-term persistence of dominant gut bacteriophage groups.
Overexpression of the AGL42 gene in cotton delayed leaf senescence through downregulation of NAC transcription factors
Authors: Latif Ayesha , Azam Saira , Shahid Naila , Javed Muhammad R. , Haider Zeshan , Yasmeen Aneela , Sadaqat Sahar , Shad Mohsin , Husnain Tayyab , Rao Abdul Q.
Published: December 2022

Premature leaf senescence negatively influences the physiology and yield of cotton plants. The conserved IDLNL sequence in the C-terminal region of AGL42 MADS-box determines its repressor potential for the down regulation of senescence-related genes. To determine the delay in premature leaf senescence, Arabidopsis AGL42 gene was overexpressed in cotton plants. The absolute quantification of transgenic cotton plants revealed higher mRNA expression of AGL42 compared to that of the non-transgenic control. The spatial expression of GUS fused with AGL42 and the mRNA level was highest in the petals, abscission zone (flower and bud), 8 days post anthesis (DPA) fiber, fresh mature leaves, and senescenced leaves. The mRNA levels of different NAC senescence-promoting genes were significantly downregulated in AGL42 transgenic cotton lines than those in the non-transgenic control. The photosynthetic rate and chlorophyll content were higher in AGL42 transgenic cotton lines than those in the non-transgenic control. Fluorescence in situ hybridization of the AG3 transgenic cotton line revealed a fluorescent signal on chromosome 1 in the hemizygous form. Moreover, the average number of bolls in the transgenic cotton lines was significantly higher than that in the non-transgenic control because of the higher retention of floral buds and squares, which has the potential to improve cotton fiber yield.
Hand function following accidental automatic animal syringe injector injuries
Authors: Rubin Guy , Feldman Guy , Shtawe Shtawe , Rozen Nimrod
Published: December 2022

Accidental self-injection injury is a common occurrence among veterinary and farm workers handling automatic syringe injectors. Most of the time, these injuries are asymptomatic or cause self-resolving mild symptoms, but these injuries may lead to significant morbidity. The aim of the study was to evaluate hand function after inadvertent injection of a poultry influenza or cholera vaccine in patients admitted to our department with infection. We retrospectively gathered data from admission to last follow-up. Functional assessment and physical exam of the hand were done at each stage by either an orthopedic resident or a fellowship-trained hand surgeon. The exam included evaluation of sensation using monofilament, joint range of motion using a goniometer, and a Quick DASH questionnaire. The study included 21 patients, all men, with a mean age of 33.4 years (range 23–44). Of the 21 patients only eight had attended all follow-ups. All patients had injury to the non-dominant hand. Seventeen of 21 of the cases had finger injuries, out of which 11 involved the thumb. The mean hospitalization time was 3.75 days (1–10). Of the 21 patients, seven underwent surgery to drain a collection during hospitalization. Seven out of eight patients had lowest disability scores on Quick Dash questionnaire. Three out of eight patients lost superficial sensation at the tip of the finger. The largest loss of range of motion was found in the distal interphalangeal joint in the finger or interphalangeal joint in the thumb, especially following surgical drainage. Of the eight patients presenting for follow-up, most had returned to the same job. Hand function was normal, as expressed in a DASH questionnaire. Sensory examination demonstrated that the sensation was almost unaffected over the injured finger. Range of motion of the joint closest to the injection site was usually the most impaired. Patients who underwent surgical drainage had a reduced range of motion.
Energy absorption characteristics of E-glass/epoxy over-wrapped aluminum pipes with induced holes: an experimental research
Authors: El-baky Marwa A. Abd , Allah Mahmoud M. Awd , kamel Madeha , Abd-Elaziem Walaa
Published: December 2022

The effect of inducing circular holes into aluminum wrapped with glass/epoxy (Al/GFRP) pipes was investigated. Intact and holed specimens were evaluated in quasi-static axial compression after being constructed using the wet wrapping procedure. The effect of the induced holes' parameters i.e., hole diameter (d), number of holes (n), the hole position to specimen height ratio (L/H), on the crashworthiness of Al/GFRP structures was investigated. Results indicated that the existence of the cutouts visibly affects the values of crushing parameters. Increasing (d) remarkably reduces the total absorbed energy (U) and enhances the crushing force efficiency (CFE) of Al/GFRP pipes. Introducing two holes in two opposite faces with keeping their location constant reduces initial peak crush force $${(\mathrm{F}}_{\mathrm{ip}})$$

(
F

ip

)

, mean crush force $${(\mathrm{F}}_{\mathrm{m}})$$

(
F

m

)

, and U of Al/GFRP pipes but increases CFE. As the hole location goes to the lower edge of the pipe i.e., L/H increases, U obviously increases. The specimens with 4 mm holes in one side at medium height and specimens with 12 mm holes on one side at L/H = 0.8 had the best crushing parameters, respectively. The adequate design of the induced cutouts in thin-walled constructions is valuable to enhance the crashworthy performance of engineering structures.
Influence of iron ore properties on dioxin emissions during iron ore sintering
Authors: Zhou Xiaoteng , Strezov Vladimir , Evans Tim , Salian Khushbu , Taylor Mark Patrick
Published: December 2022

Iron ores are principal input materials for iron and steel-making industries. Quality of iron ores is one of the critical parameters for formation of environmental pollutants related to the steel-making process. Dioxins are identified as one of the most toxic pollutants emitted during ironmaking, specifically during the sintering process. This study applied four types of iron ores and analyzed their moisture, density, particle size distribution and element concentrations to investigate their effect on the dioxin formation during sintering. Each type of iron ore was processed in a sinter pot grate. During each processing route, exhausted dust and generated sinter products were collected and subjected to PCDD/F and PCB analysis. Statistical analysis was applied to assess correlations between properties of iron ores and exhausted dioxin emissions, identifying key contributors to dioxin formation during sintering process. Results showed that Fe in iron ores was positively and significantly related to PCB 114 formation in dust and confirmed its co-catalytic effect on dioxin formation. Concentrations of Al, Ti and Cl in iron ores greatly increased PCDD/F and PCB emissions in the sintered products compared to dioxins in dust samples. The S levels and density of iron ores were highly related to the increasing PCDD/F and PCB emissions in both sinter and dust samples. By contrast, concentrations of Si in iron ores played a significant role in decreasing PCDD/F and PCB emissions in both sinter and dust samples. This study also confirmed the optimum size (< 1 mm–2.59 mm) for iron ores, which helps reduce dioxin emissions without affecting the quality of iron and steel-making products.
Proton transfer and conformational changes along the hydrogen bond network in heliorhodopsin
Authors: Tsujimura Masaki , Chiba Yoshihiro , Saito Keisuke , Ishikita Hiroshi
Published: December 2022

Heliorhodopsin releases a proton from the Schiff base during the L-state to M-state transition but not toward the protein bulk surface. Here we investigate proton transfer and induced structural changes along the H-bond network in heliorhodopsin using a quantum mechanical/molecular mechanical approach and molecular dynamics simulations. Light-induced proton transfer could occur from the Schiff base toward Glu107, reorienting Ser76, followed by subsequent proton transfer toward His80. His80 protonation induces the reorientation of Trp246 on the extracellular surface, originating from the electrostatic interaction that propagates along the transmembrane H-bond network [His80…His23…H2O[H23/Q26]…Gln26…Trp246] over a distance of 15 Å. Furthermore, it induces structural fluctuation on the intracellular side in the H-bond network [His80…Asn16…Tyr92…Glu230…Arg104…Glu149], opening the inner cavity at the Tyr92 moiety. These may be a basis of how light-induced proton transfer causes conformational changes during the M-state to O-state transition.
Present-day warm pool constrains future tropical precipitation
Authors: Park In-Hong , Yeh Sang-Wook , Min Seung-Ki , Ham Yoo-Geun , Kirtman Ben P.
Published: December 2022

Future changes in tropical precipitation affect the livelihood of the world’s human population and ecosystems. Climate models project an increased rainfall intensification under anthropogenic warming, but uncertainties in the distribution and magnitude of the changes remain large. Here, we identify a strong positive relationship between the present-day Indo-Pacific warm pool size and projected precipitation changes in the central-to-eastern tropical Pacific using multi-model simulations. Models with larger present-day warm pool size project excessive future warming in the eastern tropical Pacific due to intensified ocean stratification which reduces the zonal sea surface temperature gradient of the tropical Pacific, resulting in the weakening of Walker circulation and precipitation increases in the central-to-eastern tropical Pacific. Based on this relationship, uncertainty in the projected precipitation in the central-to-eastern tropical Pacific can be reduced by approximately 25%, which demonstrates that an improved simulation of present-day Indo-Pacific warm pool size is important for reliable tropical precipitation projections.
An integrated framework of open-source tools for designing and evaluating green hydrogen production opportunities
Authors: Khan Muhammad Haider Ali , Heywood Phoebe , Kuswara Aaron , Daiyan Rahman , MacGill Iain , Amal Rose
Published: December 2022

Extensive scaling of green hydrogen to meet net-zero targets would need the integration of suitable resources, high renewable energy potential and achievement of supporting techno-economic parameters to establish viable hydrogen projects. Herein, we propose a comprehensive four-tier framework based on specially designed open-source tools that build upon existing knowledge by providing (i) zoning filters to identify potential green hydrogen hubs, (ii) Multi-Criteria Analysis to compare and rank the selected sites, (iii) a production cost tool that allows analysis of 24 different electrolyzer – powerplant design scenarios and (iv) a python based algorithm that establishes the capacity mixes of electrolyzer, powerplant and battery energy storage system required to achieve cost or operational capacity factor targets. The framework is then applied to Australia, where 41 potential sites are used as a case study for comparing their green hydrogen generation potential and costs, benchmarked against international targets of $2 kg−1.
Performance assessment and economic analysis of a human Liver-Chip for predictive toxicology
Authors: Ewart Lorna , Apostolou Athanasia , Briggs Skyler A. , Carman Christopher V. , Chaff Jake T. , Heng Anthony R. , Jadalannagari Sushma , Janardhanan Jeshina , Jang Kyung-Jin , Joshipura Sannidhi R. , Kadam Mahika M. , Kanellias Marianne , Kujala Ville J. , Kulkarni Gauri , Le Christopher Y. , Lucchesi Carolina , Manatakis Dimitris V. , Maniar Kairav K. , Quinn Meaghan E. , Ravan Joseph S. , Rizos Ann Catherine , Sauld John F. K. , Sliz Josiah D. , Tien-Street William , Trinidad Dennis Ramos , Velez James , Wendell Max , Irrechukwu Onyi , Mahalingaiah Prathap Kumar , Ingber Donald E. , Scannell Jack W. , Levner Daniel
Published: December 2022

Background

Conventional preclinical models often miss drug toxicities, meaning the harm these drugs pose to humans is only realized in clinical trials or when they make it to market. This has caused the pharmaceutical industry to waste considerable time and resources developing drugs destined to fail. Organ-on-a-Chip technology has the potential to improve success in drug development pipelines, as it can recapitulate organ-level pathophysiology and clinical responses; however, systematic and quantitative evaluations of Organ-Chips’ predictive value have not yet been reported.

Methods

870 Liver-Chips were analyzed to determine their ability to predict drug-induced liver injury caused by small molecules identified as benchmarks by the Innovation and Quality consortium, who has published guidelines defining criteria for qualifying preclinical models. An economic analysis was also performed to measure the value Liver-Chips could offer if they were broadly adopted in supporting toxicity-related decisions as part of preclinical development workflows.

Results

Here, we show that the Liver-Chip met the qualification guidelines across a blinded set of 27 known hepatotoxic and non-toxic drugs with a sensitivity of 87% and a specificity of 100%. We also show that this level of performance could generate over $3 billion annually for the pharmaceutical industry through increased small-molecule R&D productivity.

Conclusions

The results of this study show how incorporating predictive Organ-Chips into drug development workflows could substantially improve drug discovery and development, allowing manufacturers to bring safer, more effective medicines to market in less time and at lower costs.
Baicalein-functionalized collagen scaffolds direct neuronal differentiation toward enhancing spinal cord injury repair
Authors: Qian Lin , Yang Keni , Liu Xiru , Zhang Lulu , Zhao Haitao , Qiu Lin-Zi , Chu Yun , Hao Wangping , Zhuang Yan , Chen Yanyan , Dai Jianwu
Published: December 2022

Baicalein was discovered to direct neuronal differentiation. Based on this, a baicalein functionalized collagen scaffold (BFCS) was developed for spinal cord injury (SCI) repair, which enhanced neurogenesis and motor functional recovery of SCI rats.
Nanocarriers for the delivery of antibiotics into cells against intracellular bacterial infection
Authors: Wang Chao , Yang Yi , Cao Yuanyuan , Liu Kaixin , Shi Hua , Guo Xudong , Liu Wanying , Hao Rongzhang , Song Hongbin , Zhao Rongtao
Published: December 2022

Nanocarrier systems provide a strategy to efficiently deliver antibiotics into cells against intracellular bacterial infections.
Chiral emissive porous organic cages
Authors: Sun Yu-Ling , Wang Zhen , Ma Hui , Zhang Qing-Pu , Yang Bin-Bin , Meng Xianggao , Zhang Yaohua , Zhang Chun
Published: December 2022

A pair of chiral, emissive and porous tubular multi-functional organic molecular cages were synthesized easily by imine chemistry of 4,4′,4′′,4′′′-(ethene-1,1,2,2-tetrayl)-tetrabenzaldehyde (ETTBA) with (R,R)- or (S,S)-diaminocyclohexane (CHDA).
Edge engineering on layered WS2 toward the electrocatalytic reduction of CO2: a first principles study
Authors: Tong Likai , Zhang Bo , Zhang Yu , Peng Zhijian , Fu Xiuli
Published: December 2022

Transition metal dichalcogenides (TMDCs) have been modified to show excellent electrocatalytic performance for the CO2 reduction reaction (CO2RR).
Hydration-induced protomer switching in p-aminobenzoic acid studied by cold double ion trap infrared spectroscopy
Authors: Akasaka Kyota , Hirata Keisuke , Haddad Fuad , Dopfer Otto , Ishiuchi Shun-ichi , Fujii Masaaki
Published: December 2022

Infrared spectroscopy in a double cryogenic ion trap tandem mass spectromer reveals that the correct threshold number of water molecules to trigger the hydration-induced intracluster proton transfer in protonated p-aminobenzoic acid is indeed five.
Processive binding mechanism of Cel9G from Clostridium cellulovorans: molecular dynamics and free energy landscape investigations
Authors: Li Penghui , Wang Xin , Zhang Chunchun , Xu Dingguo
Published: December 2022

The processive binding mechanism of cellulose by Cel9G from C. cellulovorans was investigated by MD and metadynamics simulations.
Edge-grafting carbon nitride with aromatic rings for highly-efficient charge separation and enhanced photocatalytic hydrogen evolution
Authors: Liu Zhang-Jie , Zhang Wei-De , Yu Yu-Xiang
Published: December 2022

Edge-modification of g-C3N4induces highly-efficient charge separation through directional transfer of electrons from the center to the edge of the framework.
Visible light-induced direct and highly selective C–H functionalization of quinoxalin-2(1H)-one without orientating group
Authors: Hou Huiqing , Wang Changsheng , Cheng Xin , Chen Houzheng , Sun Weiming , Zheng Zhiqiang , Ke Fang
Published: December 2022

Using eosin Y and KI as photocatalysts to facilitate the conversion of quinoxaline-2(1H)-one and phenylhydrazine hydrochloride compounds into corresponding 3-arylated quinoxaline-2(1H)-one, a simple and efficient synthesis method has been developed.
Uncertainty-aware and explainable machine learning for early prediction of battery degradation trajectory
Authors: Rieger Laura Hannemose , Flores Eibar , Nielsen Kristian Frellesen , Norby Poul , Ayerbe Elixabete , Winther Ole , Vegge Tejs , Bhowmik Arghya
Published: December 2022

We present an interpretable uncertainty-aware machine learning model to predict battery degradation trajectories. Using LSTM Recurrent Neural Networks, we reach an RMSE of 106 and MAPE of 10.6%.
Visualizing morphological structures of rice grains in precooked products using synchrotron radiation X-ray phase-contrast computed tomography
Authors: Miki Hiromi , Yoneyama Akio , Hirano Keiichi
Published: December 2022

The morphology and internal structure of precooked rice products were successfully visualized without contrast agents by synchrotron radiation X-ray phase-contrast computed tomography (CT).
Pericytes and shear stress each alter the shape of a self-assembled vascular network
Authors: Fujimoto Kazuya , Erickson Scott , Nakayama Masamune , Ihara Hiroki , Sugihara Kei , Nashimoto Yuji , Nishiyama Koichi , Miura Takashi , Yokokawa Ryuji
Published: December 2022

Shape changes of a vascular network in a microfluidic device with shear stress and/or pericytes were examined. The results showed maximum sprouting frequency within a specific range of shear stress regardless of flow rate, while pericytes constrained vessel diameter.
Biodegradable porous FeMn(–xAg) alloys: assessment of cytocompatibility, mechanical, magnetic and antibiofilm properties
Authors: Bartkowska Aleksandra , Careta Oriol , Turner Adam Benedict , Blanquer Andreu , Ibañez Elena , Trobos Margarita , Nogués Carme , Pellicer Eva , Sort Jordi
Published: December 2022

Equiatomic FeMn alloys with varying Ag content (1–5 wt%) and hierarchical porosity show low ferromagnetic response during biodegradation and reduction in the total biofilm biomass upon interaction withS. aureusfor 5 wt% Ag containing materials.
Water in the ball-milling process affects the dispersion of vanadia species on V2O5/TiO2 catalysts for NH3-SCR
Authors: Lian Zhihua , Zheng Mingji , Wang Qiang , Li Yingjie , Deng Feng , Shan Wenpo , He Hong
Published: December 2022

Water in the ball-milling process increased polymeric vanadyl species and enhanced the redox capability, resulting in high NH3-SCR activity of V2O5/TiO2.
Correction: AgNO3-catalyzed decarboxylative cross-coupling reaction: an approach to coenzyme Q
Authors: Wang Jin , Lu Bin , Luo Wan-Yue , Qiu Yong-Fu , Zhou Rong-Ye , He Yong-Jing
Published: December 2022

Correction for ‘AgNO3-catalyzed decarboxylative cross-coupling reaction: an approach to coenzyme Q’ by Wan-Yue Luo et al., New J. Chem., 2020, 44, 8702–8704, https://doi.org/10.1039/D0NJ01497D.
Maximizing spin Hall magnetoresistance in heavy metal/crystalline metallic ferromagnet multilayers with opposite spin Hall angles
Authors: Hui Yajuan , Jiang Hui , Xie Fei , Lin Weinan , Dong Chao , Dong Kaifeng , He Qiang , Miao Xiangshui
Published: December 2022

With the cooperative interaction of choosing the proper Ta's thickness and annealing of NiFe, a maximum SMR is obtained in Ta/Pt/NiFe trilayers. The ISOC and its reciprocal effects dominates the bulk effects.
Regulating the thermal conductivity of monolayer MnPS3 by a magnetic phase transition
Authors: Zhang Dingbo , Wang Ke , Chen Shuai , Zhang Lifa , Ni Yuxiang , Zhang Gang
Published: December 2022

In this study, based on ab initio calculations and the phonon Boltzmann transport equation, we found that magnetic phase transitions can lead to a significant change in the thermal conductivity of monolayer MnPS3.
The effect of biomolecular corona on adsorption onto and desorption from a model lipid membrane
Authors: Richards Ceri J. , Ahmadi Majid , Stuart Marc C. A. , Kooi Bart J. , Åberg Christoffer , Roos Wouter H.
Published: December 2022

Probing nanoparticle adsorption onto model lipid bilayers as a function of biomolecular corona, particle size, flow conditions and membrane composition.
Orange/cyan emissive sensors of Sb3+ for probing water via reversible phase transformation in rare-earth-based perovskite crystals
Authors: Huang Yexin , Pan Yuexiao , Peng Chengdong , Ding Yihong , Lian Hongzhou , Li Liyi , Lin Jun
Published: December 2022

A new strategy to probe water content based on orange/cyan emissive sensors of Sb3+ through the reversible phase transformation between Rb2ScCl5·H2O and Rb3ScCl6 is developed. In-depth analysis suggests that the luminescence of Sb3+ in the two crystals originates from STEs.
Alkylcarbene mediated intramolecular alkene cyclopropanation to construct aza[3.1.0] bicycles
Authors: Fu Zhen , Wang Chengming , Zhou Cong-Ying
Published: December 2022

A Rh-alkylcarbene mediated intramolecular alkene cyclopropanation has been developed. This reaction provides a rapid access to azabicyclo[3.1.0]hexanes in good to high yields.
Iron-catalyzed hydroarylation of 1,3-dienes with indoles
Authors: Huang Jie , Chen Ling-Ling , Ding Tong-Mei , Chen Zhi-Min
Published: December 2022

An efficient iron-catalyzed hydroarylation of 1,3-dienes with indoles has been developed for the first time.
Advances in electrospun nanofibrous membrane sensors for ion detection
Authors: Wu Liangqiang , Song Yan , Xing Shuo , Li Yapeng , Xu Hai , Yang Qingbiao , Li Yaoxian
Published: December 2022

Harmful metal ions and toxic anions produced in industrial processes cause serious damage to the environment and human health.
Engineering NiCo2S4 nanoparticles anchored on carbon nanotubes as superior energy-storage materials for supercapacitors
Authors: Chen Junming , Du Zhiling , Cheng Kun , Bao Jusheng , Wang Guiling , Yao Yue , Song Jiayi , Yue Jing , Xu Kun , Xie Weicheng , Qiang Wei , Liu You , Wang Xuchun
Published: December 2022

A one-step hydrothermal method was used to successfully synthesize NiCo2S4 nanocomposites anchored on carbon nanotubes as excellent energy storage materials for supercapacitors.
Two-stage one-pot synthetic strategy for the key triazone-triazole intermediate of ensitrelvir (S-217622), an oral clinical candidate for treating COVID-19
Authors: Hu Wei , Zhang Xiang , Liu Yuanchang , Liu Teng , Wen Jiale , Peng Xiaopeng , Xie Xin , Chen Weiming
Published: December 2022

The key triazone-triazole intermediate of ensitrelvir (S-217622), an oral clinical candidate for treating COVID-19, was synthesized via two-stage one-pot strategy with high efficiency.
Time- and angle-resolved photoemission spectroscopy (TR-ARPES) of TMDC monolayers and bilayers
Authors: Liu Fang
Published: December 2022

Many unique properties in two-dimensional (2D) materials and their heterostructures rely on charge excitation, scattering, transfer and relaxation dynamics across different points in the momentum space.
Electronic structure comparisons of isostructural early d- and f-block metal(iii) bis(cyclopentadienyl) silanide complexes
Authors: Gransbury Gemma K. , Réant Benjamin L. L. , Wooles Ashley J. , Emerson-King Jack , Chilton Nicholas F. , Liddle Stephen T. , Mills David P.
Published: December 2022

We report an isostructural series of early metal complexes containing cyclopentadienyl (Cp)-supported M–Si bonds. Differences in the extent of metal–ligand covalency causes a magnetic anisotropy switch between d- and f-block metals.
Fluorescent organometallic dyads and triads: establishing spatial relationships
Authors: Shinozaki Yoshinao , Popov Stepan , Plenio Herbert
Published: December 2022

Bodipy-based organometallic dyads and triads are synthesized and FRET is shown to provide information on the formation of various gold complexes.
The kinked structure and interchain van der Waals interaction of carbyne nanocrystals
Authors: Cao Weiwei , Xu Huakai , Liu Pu , He Yan , Yang Guowei
Published: December 2022

Carbyne with one-dimensional sp-hybridized carbon atoms is the third form of carbon following diamond and graphite.
A crystal growth kinetics guided Cu aerogel for highly efficient CO2electrolysis to C2+alcohols
Authors: Li Pengsong , Bi Jiahui , Liu Jiyuan , Zhu Qinggong , Chen Chunjun , Sun Xiaofu , Zhang Jianling , Liu Zhimin , Han Buxing
Published: December 2022

Controlling crystal growth kinetics using different reductants is an efficient strategy to modulate the surficial chemistry micro-circumstance of Cu aerogels, enabling the creation of efficient surface sites for increasing C2+product selectivity.
Polymeric encapsulation of a ruthenium(ii) polypyridyl complex: from synthesis to in vivo studies against high-grade epithelial ovarian cancer
Authors: António João P. M. , Gandioso Albert , Nemati Fariba , Soliman Nancy , Vinck Robin , Sun Fan , Robert Carine , Burckel Pierre , Decaudin Didier , Thomas Christophe M. , Gasser Gilles
Published: December 2022

A simple, one-pot procedure for the preparation of covalently-attached Ru–polylactide nanoparticles is reported.
Label-free quantification of passive membrane permeability of cyclic peptides across lipid bilayers: penetration speed of cyclosporin A across lipid bilayers
Authors: Ono Takahiro , Tabata Kazuhito V. , Goto Yuki , Saito Yutaro , Suga Hiroaki , Noji Hiroyuki , Morimoto Jumpei , Sando Shinsuke
Published: December 2022

We developed a label-free lipid bilayer permeability assay, which realized the measurement of the penetration speed of cyclosporin A.
Surfactant-free synthesis of metal and metal oxide nanomaterials: a perspective
Authors: Iravani Siavash
Published: December 2022

Recent developments pertaining to the surfactant-free synthesis of metal and metal oxide nanomaterials are deliberated, with a focus on important challenges, opportunities, and future perspectives.
Thermocatalytic CO2conversion by siliceous matter: a review
Authors: Kosari Mohammadreza , Lim Alvin M. H. , Shao Yu , Li Bowen , Kwok Kelvin M. , Seayad Abdul Majeed , Borgna Armando , Zeng Hua Chun
Published: December 2022

Silica/silicate-based nanocatalysts have shown great potential for catalytic hydrogenation of CO2to various value-added chemicals. This review presents the recent development of siliceous matter in this important field of heterogeneous catalysis.
Li+ storage and transport in high-voltage spinel-type LiNi0.5Mn1.5O4 codoped with F− and Cu2+
Authors: Kim Dae-wook , Shiiba Hiromasa , Teshima Katsuya , Zettsu Nobuyuki
Published: December 2022

Material design based on the F−/Cu2+-codoping into the spinel lattice of LiNi0.5Mn1.5O4 enable to attain high voltage durability and high reversible discharge capacity of >200 mA h g−1 at cut-off voltages of 4.8–2.0 V (vs. Li+/Li).
Nanofibrillar hydrogels outperform Pt/C for hydrogen evolution reactions under high-current conditions
Authors: Park Jinwoo , Jeon Dasom , Kang Yunseok , Ryu Jungki , Lee Dong Woog
Published: December 2022

A nanofibrous hydrogel-embedded Ni foam (NiF) electrode outperforms those modified with commercial Pt/C catalysts under practically meaningful high-current conditions by lowering the adhesion force and facilitating the removal of adsorbed gas bubbles.
Mesoporous bioglass capsule composite injectable hydrogels with antibacterial and vascularization promotion properties for chronic wound repair
Authors: Wei Xuejie , Zhuang Pengzhen , Liu Kun , Hou Wen , Zhao Yanan , Wei Wenying , Tu Rong , Li Haiwen , Dai Honglian
Published: December 2022

MBG@PDA composite hydrogels repair chronic wounds by establishing a microenvironment for angiogenesis and inhibiting wound infection.
Tissue-engineered vascular graft based on a bioresorbable tubular knit scaffold with flexibility, durability, and suturability for implantation
Authors: Lee Dong Yeop , Jang Yongwoo , Kim Eunyoung , Li Tao , Kim Seon Jeong
Published: December 2022

The tissue-engineered vascular graft (TEVG) is a technology used to recreate a blood vessel by using vascular cells and scaffolds, which is a promising approach as a feasible alternative for small-diameter blood vessel replacement.
Rapid high-contrast reversible coloration of Ba3MgSi2O8:Pr3+ photochromic materials for rewritable light-printing
Authors: Tang Wei , Zuo Chuandong , Ma Chaoyang , Chang Chen , Dang Fan , Liu Hao , Li Yingkui , Yuan Xuanyi , Wen Zicheng , Wu Lijun , Cao Yongge
Published: December 2022

A “rewritable paper” based on Ba3MgSi2O8 inorganic photochromic material presents a series of advantages such as large photochromic contrast (77.1%), fast response time (3 s), strong fatigue resistance and stable physical/chemical properties.
A cysteamine-functionalized biomimetic chromotropic hydrogel for naked-eye detection and adsorption of mercury ions
Authors: Liu Bing , Zhao Feng , Qiu Yang , Liu Wenzhao , Wu Zhaoyang
Published: December 2022

Herein, inspired by chameleons, a cysteamine-functionalized biomimetic chromotropic hydrogel (Cys-BCH) capable of simultaneous naked-eye detection and adsorption of Hg2+ is ingeniously constructed.
Use and Perceived Effectiveness of Communication Modes Reported by Persons With Primary Progressive Aphasia
Authors: Mooney Aimee R. , Bravo Megan , Roberts Angela , Salley Elizabeth , Blaze Erin , Esparza Marissa , Fried-Oken Melanie , Khayum Becky , Rao Leela , Rademaker Alfred , Rogalski Emily
Published: December 2022

Purpose:

Primary progressive aphasia (PPA) is a clinical neurodegenerative dementia syndrome characterized by early, selective, and progressive language impairment. PPA onset is gradual, providing time to potentially identify additional or alternative expressive communication modes; however, reports of communication mode use and effectiveness by persons with PPA have not been described. This study characterized the use, frequency, and perceived effectiveness of communication modes reported by individuals with PPA.

Method:

Forty-one participants with mild-to-moderate PPA completed a structured interview detailing the type, frequency, and perceived effectiveness of 12 potential communication modes, categorized by technology required (no-tech, low-tech, and high-tech). The ratio of modes used was compared across technology categories with a repeated-measures generalized linear model assuming a binomial distribution with an overall Wald chi-square statistic, followed by pairwise post hoc
t
-test comparisons.

Results:

Of the 12 communication modes assessed, participants reported using a median of eight (range: 5–10). All participants affirmed using speech, facial expressions, and talking on the phone. Frequency and perceived effectiveness ratings for these three modes were endorsed at the “some/most of the time” level for more than 80% of the participants. No-tech mode use was significantly higher than reported high-tech and low-tech modes (
p
= .004 and
p
< .0001, respectively). Even so, while some high-tech modes (apps) and some low-tech modes (nonelectronic augmentative and alternative communication) had fewer users, effectiveness ratings were moderate to high for all but one user.

Conclusions:

Persons with mild-to-moderate language impairment due to PPA report using a range of communication modes with moderate-to-high frequency and perceived effectiveness. These outcomes provide practical information when considering mode refinement or expansion during intervention to maximize communication participation. Barriers to modality use may include low awareness or access, which could be queried by future studies and supported by speech and language interventions.

Supplemental Material:

https://doi.org/10.23641/asha.21614262
Minimum inertia demand estimation of new power system considering diverse inertial resources based on deep neural network
Authors: Zicheng Liu , Zhou Tao , Chen Zhong , Wang Yi , Wang Yalun
Published: December 2022

With the high‐proportion integration of renewable energy and power electronic equipment, the inertia supporting ability of new power system continues to decline, which seriously threatens the frequency stability of power grids. In order to clarify the operation boundary, and realise the rapid analysis and prediction of the minimum inertia demand of new power systems, this study proposes a minimum inertia demand estimation method based on deep neural network (DNN). Firstly, this study establishes the system frequency response model of new power systems containing diverse inertia resources including renewable energy, induction machine and so on. Considering the constraints of rate of change of frequency and maximum frequency deviation, the minimum inertia demand estimation model is established to ensure the system frequency stability. DNN is introduced to effectively map non‐linear relations in complex situations, which can quickly estimate and predict the minimum inertia of new power systems. Adam algorithm is utilised to optimise the input weight matrix and hidden layer feature vector of the network to improve accuracy. Finally, the simulations and analysis are conducted in IEEE‐39 system to verify the accuracy and generalisation ability of the proposed method in this paper.