bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental Study on the Mechanical Behaviors of Loess Reinforced with Randomly Distributed Basalt Fiber
Authors: Kou Honggang , Ma Qiang , Han Shunli
Published: September 2022

Loess has the structural characteristics of porous, weakly cemented and under compacted, leading to its collapsible, disintegrative and dissolute features. To study the mechanical behaviors of basalt fiber-reinforced loess, consolidated undrained triaxial tests were carried out to investigate the effects of fiber length (FL), fiber content (FC) and cell pressure (σ3) on the shear strength. Based on the test results, a constitutive model considering the effects of the σ3, FL and FC was established using regression analysis, and the estimation method for the model parameters was proposed. The results show that the stress–strain curve of the unreinforced loess exhibited a strain-softening type, while the reinforced loess displayed a strain-hardening type. The peak strength of the reinforced loess was significantly higher than that of the unreinforced soil, and increased with increasing of FL, FC and σ3. Compared with the peak strength when FL was 8 mm, the peak strength increased slightly when the FL was 12 and 16 mm, respectively. The anchoring effect and bridging effect between soil particles and fibers improved the cohesion and friction of reinforced soil, resulting in the increment in the shear strength. The experimental results are in good agreement with the model predictions, indicating that the established model and the parameter estimation method are suitable for describing the relationship between the stress and strain of basalt-fiber-reinforced loess. The research results can provide guidance of the design and construction of fiber-reinforced soil in loess areas.
The “Weekend Effect” in Lightning Activity during Winter Thunderstorms over the Tel-Aviv, Israel, Metropolitan Area
Authors: Yair Yoav Y. , Lynn Barry H. , Korzets Menahem , Jaffe Mordecai
Published: September 2022

We report the analysis of nine winter seasons (December, January, February (DJF)) aerosol and lightning data over the Tel-Aviv metropolitan area, Israel’s largest and densest urban region. Hourly averaged aerosol concentrations (PM2.5 and PM10) were obtained from 27 automatic air quality stations operated by the Ministry for Environmental Protection. Lightning data obtained from the Israeli Lightning Detection Network (ILDN) and by the Earth Network Total Lightning Network (ENTLN) for four overlapping seasons showed an irregular pattern, with lower activity during Sunday–Tuesday, and maximum activity on Wednesday and Thursday, but also on Saturday, when less pollution was present. The accepted explanation for the weekend effect is that increased amounts of particles tend to prolong the lifetime of storms and their total lightning amounts and change their intracloud/cloud-to-ground flash ratios. However, our results suggest that lightning is not directly related to the level of ambient pollution, but likely depends on the synergistic effects of desert dust and urban pollution particles in the area that affect the electrical structure of winter thunderstorms. Since the source of desert dust is independent of any regularity that can be attributed to anthropogenic activity (e.g., increased levels of pollution due to the traffic-load in Tel-Aviv), it obscures any weekend–weekday patterns that are often detected in other large metropolitan areas.
Graduation Towers Impact on the Concentration and Chemical Composition of Ambient Aerosol: A Case Study from Wieliczka Salt Mine in Poland
Authors: Rogula-Kozłowska Wioletta , Badyda Artur , Rachwał Marzena , Rogula-Kopiec Patrycja , Majder-Łopatka Małgorzata , Kostrzon Magdalena , Mathews Barbara
Published: September 2022

The aim of the research was to determine the effect of graduation towers on the aerosol concentration and its composition not only in the vicinity of the graduation tower itself, but also at a distance from it, on the example of the brine graduation tower in the Wieliczka Salt Mine health resort. Two measurement sites were selected for the research, one located inside the graduation tower and the other at the guard booth—at a considerable distance from the graduation tower. Total suspended particulate (TSP) and PM10 (particulate matter with a diameter that does not exceed 10 µm) samples were taken simultaneously using the aspiration method. The collected samples were subjected to analyses of TSP and PM10 concentrations, the content of organic carbon (OC), elemental carbon (EC) and selected ions. It was confirmed that the composition of the aerosol in the Wieliczka Salt Mine significantly differs from the typical aerosol composition in inland locations and is similar to the aerosol composition in coastal zones. The comparison of the aerosol composition at both measuring sites clearly indicates a very favorable influence of the brine graduation tower on the composition of the atmospheric aerosol, even at a certain distance from it.
Analysis of the Carbon Intensity of Container Shipping on Trunk Routes: Referring to the Decarbonization Trajectory of the Poseidon Principle
Authors: Tai Hui-Huang , Chang Yun-Hua , Chang Chin-Wei , Wang Yu-Meng
Published: September 2022

Container shipping industries are highly capital intensive. If shipping carriers want to execute international shipping financing, they must follow the IMO emission reduction targets and meet the decarbonization trajectory of the Poseidon Principle (PP). This article used an activity-based model to calculate container shipping industry carbon emissions. It was found that the carbon intensity per unit for each ship was decreased because of the upsizing of container vessels and route deployment based on the alliance strategy. On the Asia–Europe (A/E) trunk route, as the ship size increased from 11,300 to 24,000 TEU, the results showed that the carbon intensity ranged from 6.48 to 3.06 g/ton-nm. It is also proven that the mega-container deployment on the A/E trunk route followed the decarbonization trajectory proposed by PP, while the Asia–Pacific trunk route was not fully in line with the trajectory of EEOI/AER. It is worth noting that starting from 2020, due to the COVID-19 pandemic, shipping companies deployed a higher number of small-size vessels to boost revenues, resulting in more pollutants produced and a mismatch of the trajectory proposed by PP.
Using Molecular Lines to Determine Carbon and Nitrogen Abundances in the Atmospheres of Cool Stars
Authors: Ryabchikova Tatiana , Piskunov Nikolai , Pakhomov Yury
Published: September 2022

Simultaneous analysis of the C2 and CN molecular bands in the 5100–5200 and 7930–8100 Å spectral regions is a promising alternative for the accurate determination of the carbon (C) and nitrogen (N) abundance in the atmospheres of the solar-like stars. Practical implementation of this new method became possible after recent improvements of the molecular constants for both molecules. The new molecular data predicted the correct line strength and line positions; therefore, they were included in the Vienna Atomic Line Database (VALD), which is widely used by astronomers and spectroscopists. In this paper, we demonstrate that the molecular data analysis provides C and, in particular, N abundances consistent with those derived from the atomic lines. We illustrate this by performing the analysis for three stars. Our results provide strong arguments for using the combination of C2 and CN molecular lines for accurate nitrogen abundance determination keeping in mind the difficulties of using the N i lines in the observed spectra of the solar-like stars.
A New 3-Parameter Bounded Beta Distribution: Properties, Estimation, and Applications
Authors: Althubyani Faiza A. , Abd El-Bar Ahmed M. T. , Fawzy Mohamad A. , Gemeay Ahmed M.
Published: September 2022

This study presents a new three-parameter beta distribution defined on the unit interval, which can have increasing, decreasing, left-skewed, right-skewed, approximately symmetric, bathtub, and upside-down bathtub shaped densities, and increasing, U, and bathtub shaped hazard rates. This model can define well-known distributions with various parameters and supports, such as Kumaraswamy, beta exponential, exponential, exponentiated exponential, uniform, the generalized beta of the first kind, and beta power distributions. We present a comprehensive account of the mathematical features of the new model. Maximum likelihood methods and a Bayesian method under squared error and linear exponential loss functions are presented; also, approximate confidence intervals are obtained. We present a simulation study to compare all the results. Two real-world data sets are analyzed to demonstrate the utility and adaptability of the proposed model.
Spaces of Bounded Measurable Functions Invariant under a Group Action
Authors: Hokamp Samuel A.
Published: September 2022

In this paper, we characterize spaces of L∞-functions on a compact Hausdorff space that are invariant under a transitive and continuous group action. This work is analogous to established results concerning invariant spaces of continuous and measurable functions on a compact Hausdorff space. The case for L∞-functions cannot be proved in the same way when endowed with the norm-topology, but a similar argument can be used when the space of L∞-functions is given the weak*-topology, as we show in this paper.
Applications of Higher-Order q-Derivative to Meromorphic q-Starlike Function Related to Janowski Function
Authors: Liu Likai , Srivastava Rekha , Liu Jin-Lin
Published: September 2022

By making use of a higher-order q-derivative operator, certain families of meromorphic q-starlike functions and meromorphic q-convex functions are introduced and studied. Several sufficient conditions and coefficient inequalities for functions in these subclasses are derived. The results presented in this article extend and generalize a number of previous results.
Applications of the q-Sălăgean Differential Operator Involving Multivalent Functions
Authors: Alb Lupaş Alina
Published: September 2022

In this article we explore several applications of q-calculus in geometric function theory. Using the method of differential subordination, we obtain interesting univalence properties for the q-Sălăgean differential operator. Sharp subordination results are obtained by using functions with remarkable geometric properties as subordinating functions and considering the conditions of expressions involving the q-Sălăgean differential operator and a convex combination using it.
Graft Diameter Should Reflect the Size of the Native Anterior Cruciate Ligament (ACL) to Improve the Outcome of ACL Reconstruction: A Finite Element Analysis
Authors: Wang Huizhi , Tao Mingzhu , Shi Qinyi , He Kaixin , Cheng Cheng-Kung
Published: September 2022

The size of the anterior cruciate ligament (ACL) often varies between individuals, but such variation is not typically considered during ACL reconstruction (ACLR). This study aimed to explore how the size of the ACL affects the selection of a suitable graft diameter. A finite element model of a human knee was implanted with intact ACLs of different dimensions (0.95, 1 and 1.05 times the size of the original ACL) and with grafts of different diameters, to simulate ACLR (diameter 7.5–12 mm in 0.5 mm increments). The knee models were flexed to 30° and loaded with an anterior tibial load of 103 N, internal tibial moment of 7.5 Nm, and valgus tibial moment of 6.9 Nm. Knee kinematics (anterior tibial translation (ATT), internal tibial rotation (ITR) and valgus tibial rotation (VTR)) and ligament forces were recorded and compared among the different groups. The results showed that, compared with the intact knee, a graft diameter of 7.5 mm was found to increase the ATT and VTR, but reduce the graft force. Increasing the graft diameter reduced knee laxity and increased the graft force. A 10% increase in the size of the ACL corresponded to a 3 mm larger graft diameter required to restore knee stability and graft force after ACLR. It was concluded that the graft diameter should be selected according to the dimensions of the native ACL, for better restoration of knee functionality. This study may help to improve the clinical treatment of ACL ruptures.
Tail Wags Dog’s SINE: Retropositional Mechanisms of Can SINE Depend on Its A-Tail Structure
Authors: Kosushkin Sergei A. , Ustyantsev Ilia G. , Borodulina Olga R. , Vassetzky Nikita S. , Kramerov Dmitri A.
Published: September 2022

SINEs, non-autonomous short retrotransposons, are widespread in mammalian genomes. Their transcripts are generated by RNA polymerase III (pol III). Transcripts of certain SINEs can be polyadenylated, which requires polyadenylation and pol III termination signals in their sequences. Our sequence analysis divided Can SINEs in canids into four subfamilies, older a1 and a2 and younger b1 and b2. Can_b2 and to a lesser extent Can_b1 remained retrotranspositionally active, while the amplification of Can_a1 and Can_a2 ceased long ago. An extraordinarily high Can amplification was revealed in different dog breeds. Functional polyadenylation signals were analyzed in Can subfamilies, particularly in fractions of recently amplified, i.e., active copies. The transcription of various Can constructs transfected into HeLa cells proposed AATAAA and (TC)n as functional polyadenylation signals. Our analysis indicates that older Can subfamilies (a1, a2, and b1) with an active transcription terminator were amplified by the T+ mechanism (with polyadenylation of pol III transcripts). In the currently active Can_b2 subfamily, the amplification mechanisms with (T+) and without the polyadenylation of pol III transcripts (T−) irregularly alternate. The active transcription terminator tends to shorten, which renders it nonfunctional and favors a switch to the T− retrotransposition. The activity of a truncated terminator is occasionally restored by its elongation, which rehabilitates the T+ retrotransposition for a particular SINE copy.
Bioprospecting for Novel Probiotic Strains from Human Milk and Infants: Molecular, Biochemical, and Ultrastructural Evidence
Authors: Mahmoud Sabry Y. M. , Atallah Atallah A. , Badr Omnia A. , Moustafa Mahmoud M. A. , Esmael Ahmed , Ebrahim Nesrine , Aljeldah Mohammed , Al Shammari Basim , Alsafari Ibrahim A. , Mohamed Shereen A.
Published: September 2022

Human milk comprises a diverse array of microbial communities with health-promoting effects, including colonization and development of the infant’s gut. In this study, we characterized the bacterial communities in the Egyptian mother–infant pairs during the first year of life under normal breastfeeding conditions. Out of one hundred isolates, forty-one were chosen for their potential probiotic properties. The selected isolates were profiled in terms of morphological and biochemical properties. The taxonomic evidence of these isolates was investigated based on 16S rRNA gene sequence and phylogenetic trees between the isolates’ sequence and the nearest sequences in the database. The taxonomic and biochemical evidence displayed that the isolates were encompassed in three genera: Lactobacillus, Enterococcus, and Lactococcus. The Lactobacillus was the most common genus in human milk and feces samples with a high incidence of its different species (Lacticaseibacillus paracasei, Lactobacillus delbrueckii, Lactiplantibacillus plantarum, Lactobacillus gasseri, and Lacticaseibacillus casei). Interestingly, BlastN and Jalview alignment results evidenced a low identity ratio of six isolates (less than 95%) with database sequences. This divergence was supported by the unique physiological, biochemical, and probiotic features of these isolates. The isolate L. delbrueckii, ASO 100 exhibited the lowest identity ratio with brilliant probiotic and antibacterial features suggesting the high probability of being a new species. Nine isolates were chosen and subjected to probiotic tests and ultrastructural analysis; these isolates exhibited antibiotic resistance and antibacterial activity with high probiotic characteristics, and high potentiality to be used as prophylactic and therapeutic agents in controlling intestinal pathogens.
Galectin-10 as a Potential Biomarker for Eosinophilic Diseases
Authors: Tomizawa Hiroki , Yamada Yoshiyuki , Arima Misaki , Miyabe Yui , Fukuchi Mineyo , Hikichi Haruka , Melo Rossana C. N. , Yamada Takechiyo , Ueki Shigeharu
Published: September 2022

Galectin-10 is a member of the lectin family and one of the most abundant cytoplasmic proteins in human eosinophils. Except for some myeloid leukemia cells, basophils, and minor T cell populations, galectin-10 is exclusively present in eosinophils in the human body. Galectin-10 forms Charcot–Leyden crystals, which are observed in various eosinophilic diseases. Accumulating studies have indicated that galectin-10 acts as a new biomarker for disease activity, diagnosis, and treatment effectiveness in asthma, eosinophilic esophagitis, rhinitis, sinusitis, atopic dermatitis, and eosinophilic granulomatosis with polyangiitis. The extracellular release of galectin-10 is not mediated through conventional secretory processes (piecemeal degranulation or exocytosis), but rather by extracellular trap cell death (ETosis), which is an active cell death program. Eosinophils undergoing ETosis rapidly disintegrate their plasma membranes to release the majority of galectin-10. Therefore, elevated galectin-10 levels in serum and tissue suggest a high degree of eosinophil ETosis. To date, several studies have shown that galectin-10/Charcot–Leyden crystals are more than just markers for eosinophilic inflammation, but play functional roles in immunity. In this review, we focus on the close relationship between eosinophils and galectin-10, highlighting this protein as a potential new biomarker in eosinophilic diseases.
A Micro-CT Analysis of Initial and Long-Term Pores Volume and Porosity of Bioactive Endodontic Sealers
Authors: Radwanski Mateusz , Leski Michal , Puszkarz Adam K. , Sokolowski Jerzy , Hardan Louis , Bourgi Rim , Sauro Salvatore , Lukomska-Szymanska Monika
Published: September 2022

The evaluation of the porosities within the interface of root canals obturated with endodontics materials is extremely important for the long-term success of endodontic treatments. The aim of this study was to compare initial and long-term volume of pores (total, open, closed) and porosity (total, regional) of three bioactive endodontic sealers: GuttaFlow Bioseal, Total Fill BC Sealer, and BioRoot RCS. Root canals were obturated with three “bioactive” sealers using the single-cone technique. The volume of open and closed pores and porosity were calculated using a micro-computed tomography (MCT) method. The measurements were performed after 7 days (initial) and after 6 months (long-term) of incubation. Statistical significance was considered at p < 0.05. The total volume of pores remained unchanged after the 6-month storage. GuttaFlow Bioseal exhibited significantly higher long-term volume in open pores than Total Fill BC Sealer. The total porosity in all the tested sealers presented no statistically significant change after the 6-month storage, except for BioRoot RCS. The total porosity values of this latter material significantly increased after long-term incubation, especially in the apical region. In conclusion, the use of bioactive sealers with excessive tendency to create porosities both in shorth- and long-term periods of storage may compromise the long-term success of endodontic treatments.
High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood
Authors: Krueger Kimberly , Mayer Zsuzsanna , Gerckens Miriam , Boeck Stefan , Luppa Peter , Holdenrieder Stefan
Published: September 2022

Programmed death-1 receptor PD-1(CD279) and its corresponding ligands PD-L1(CD274, B7-H1) and PD-L2(CD273, B7-DC) play important roles in physiological immune tolerance and for immune escape in cancer disease. Hence, the establishment and analytical validation of a novel enzyme-linked immunosorbent assay (ELISA) to measure soluble PD-1, PD-L1 and PD-L2 in blood samples according to high quality standards is required. Antibody pairs were used to establish novel highly sensitive ELISAs for all three markers on an open electrochemiluminescence Quickplex platform. Analytical validation comprised intra- and interassay imprecision, limit of quantification, dilution linearity, material comparison and analytical selectivity testing. The methods demonstrated a broad dynamic range and precise measurements down to the pg/mL range. The coefficient of variation (CV) during the intra-assay imprecision measurements with three patient pools did not exceed 10% for all three assays (PD-1: 6.4%, 6.5%, 7.8%, PD-L1: 7.1%, 4.2%, 6.8%; PD-L2: 4.5%, 10.0%, 9.9%). Dilution linearity experiments in both buffer and heparin plasma displayed good linearity. Selectivity was shown for each marker in titration cross-reactivity experiments up to concentrations of at least 15 ng/mL of these, possibly confounding other markers. Soluble PD-1, PD-L1 and PD-L2 can be measured highly sensitively in serum and plasma and can safely be applied to clinical study settings.
Diagnostic Accuracy of Ultrasound in Predicting Extrathyroidal Extension and Its Relation to Body Mass Index in a North American Population
Authors: Omar Mahmoud , Attia Abdallah S. , Issa Peter P. , Christensen Bryce R. , Sugumar Kavin , Alnahla Ahmed , Hadedeya Deena , Shalaby Hosam , Gupta Neel , Shama Mohamed , Toraih Eman , Kandil Emad
Published: September 2022

Detection of extrathyroidal extension (ETE) in patients with papillary thyroid carcinoma (PTC) influences treatment plan and surgical aggressiveness. Ultrasound (US) is the long-standing preoperative imaging method of choice. Recent literature from Asia suggests US accuracy to be influenced by patient characteristics, such as body mass index (BMI). Here, we examine the effect of BMI on the accuracy of US at a North American tertiary referral center. A total of 204 PTC-confirmed patients were retrospectively read by a radiologist blinded to surgical pathology findings. The radiologist recorded multiple sonographic features, including ETE, loss of echogenic capsule, nodule vascularity, capsular abutment, and bulging of contour. When considering all patients, the ultrasonographic feature with the best overall performance was loss of echogenic capsule (diagnostic odds ratio (DOR) = 4.48, 95% confidence interval (CI) = 1.86–10.78). Sub-group analysis by patient BMI found that area under the curve (AUC) for sonographic features was greater in non-obese BMI patients (0.71 ± 0.06) when compared with obese patients (0.43 ± 0.05; p = 0.001). Overall, US diagnostic performance was significantly better in non-obese (DOR = 3.70, 95%CI = 1.53–8.94) patients when compared to those who were obese (DOR = 1.12, 95%CI = 0.62–2.03; p = 0.03). Loss of the echogenic capsule did not differ between the two cohorts with respect to DOR (p = 0.51), specificity (p = 0.52), or sensitivity (p = 0.09). Our work suggests that the diagnostic value of ETE detection by US is impaired in obese patients. Considering that loss of the echogenic capsule did not differ with respect to diagnostic performance, specificity, nor sensitivity between non-obese and obese patients, it could be considered the most important predictor of US-determined ETE.
Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex
Authors: Razza Lais Boralli , da Silva Pedro Henrique Rodrigues , Busatto Geraldo F. , Duran Fábio Luis de Souza , Pereira Juliana , De Smet Stefanie , Klein Izio , Zanão Tamires A. , Luethi Matthias S. , Baeken Chris , Vanderhasselt Marie-Anne , Buchpiguel Carlos Alberto , Brunoni André Russowsky
Published: September 2022

Non-invasive brain stimulation (NIBS) interventions are promising for the treatment of psychiatric disorders. Notwithstanding, the NIBS mechanisms of action over the dorsolateral prefrontal cortex (DLPFC), a hub that modulates affective and cognitive processes, have not been completely mapped. We aimed to investigate regional cerebral blood flow (rCBF) changes over the DLPFC and the subgenual anterior cingulate cortex (sgACC) of different NIBS protocols using Single-Photon Emission Computed Tomography (SPECT). A factorial, within-subjects, double-blinded study was performed. Twenty-three healthy subjects randomly underwent four sessions of NIBS applied once a week: transcranial direct current stimulation (tDCS), intermittent theta-burst stimulation (iTBS), combined tDCS + iTBS and placebo. The radiotracer 99m-Technetium-ethylene-cysteine-dimer was injected intravenously during the NIBS session, and SPECT neuroimages were acquired after the session. Results revealed that the combination of tDCS + iTBS increased right sgACC rCBF. Cathodal and anodal tDCS increased and decreased DLPFC rCBF, respectively, while iTBS showed no significant changes compared to the placebo. Our findings suggest that the combined protocol might optimize the activity in the right sgACC and encourage future trials with neuropsychiatric populations. Moreover, mechanistic studies to investigate the effects of tDCS and iTBS over the DLPFC are required.
A Retrospective Comparison of Three Patient-Controlled Analgesic Strategies: Intravenous Opioid Analgesia Plus Abdominal Wall Nerve Blocks versus Epidural Analgesia versus Intravenous Opioid Analgesia Alone in Open Liver Surgery
Authors: Tsai Hsin-I , Lu Yu-Chieh , Zheng Chih-Wen , Yu Ming-Chin , Chou An-Hsun , Lee Cheng-Han , Kou Hao-Wei , Lin Jr-Rung , Lai Yu-Hua , Chang Li-Ling , Lee Chao-Wei
Published: September 2022

Background: Adequate pain control is of crucial importance to patient recovery and satisfaction following abdominal surgeries. The optimal analgesia regimen remains controversial in liver resections. Methods: Three groups of patients undergoing open hepatectomies were retrospectively analyzed, reviewing intravenous patient-controlled analgesia (IV-PCA) versus IV-PCA in addition to bilateral rectus sheath and subcostal transversus abdominis plane nerve blocks (IV-PCA + NBs) versus patient-controlled thoracic epidural analgesia (TEA). Patient-reported pain scores and clinical data were extracted and correlated with the method of analgesia. Outcomes included total morphine consumption and numerical rating scale (NRS) at rest and on movement over the first three postoperative days, time to remove the nasogastric tube and urinary catheter, time to commence on fluid and soft diet, and length of hospital stay. Results: The TEA group required less morphine over the first three postoperative days than IV-PCA and IV-PCA + NBs groups (9.21 ± 4.91 mg, 83.53 ± 49.51 mg, and 64.17 ± 31.96 mg, respectively, p < 0.001). Even though no statistical difference was demonstrated in NRS scores on the first three postoperative days at rest and on movement, the IV-PCA group showed delayed removal of urinary catheter (removal on postoperative day 4.93 ± 5.08, 3.87 ± 1.31, and 3.70 ± 1.30, respectively) and prolonged length of hospital stay (discharged on postoperative day 12.71 ± 7.26, 11.79 ± 5.71, and 10.02 ± 4.52, respectively) as compared to IV-PCA + NBs and TEA groups. Conclusions: For postoperative pain management, it is expected that the TEA group required the least amount of opioid; however, IV-PCA + NBs and TEA demonstrated comparable postoperative outcomes, namely, the time to remove nasogastric tube/urinary catheter, to start the diet, and the length of hospital stay. IV-PCA with NBs could thus be a reliable analgesic modality for patients undergoing open liver resections.
Waist-to-Hip Ratio and Inflammatory Parameters Are Associated with Risk of Non-Alcoholic Fatty Liver Disease in Patients with Morbid Obesity
Authors: Borges-Canha Marta , Neves João , Silva Maria , Mendonça Fernando , Moreno Telma , Ribeiro Sara , Correa João , Vale Catarina , Gonçalves Juliana , Urbano Ferreira Helena , Gil-Santos Sara , Guerreiro Vanessa , Sande Ana , B. Souto Selma , Pedro Jorge , Freitas Paula , Carvalho Davide ,   CRIO Group
Published: September 2022

Non-alcoholic fatty liver disease (NAFLD) is associated with several other metabolic disorders, which are typically pro-inflammatory states. Body fat content is an important marker of metabolic health and abdominal fat is associated with harmful cardiometabolic outcomes. We aimed to evaluate the association between the risk of NAFLD (through Fatty Liver Index (FLI), and BMI, AST/ALT ratio, and presence of diabetes (BARD)), and anthropometric parameters, predictors of metabolic status, in patients with morbid obesity, and to evaluate the association of FLI and BARD scores with pro-inflammatory markers. We have retrospectively studied patients with morbid obesity followed in our center. In total, 2184 participants were included, with an average age of 42.8 ± 10.6 years, 84.5% being females. We report a positive association of FLI with waist circumference (β = 0.10 [0.09 to 0.11], p < 0.01) and waist-to-hip ratio (β = 8.68 [6.85 to 10.52, p < 0.01]), even after adjusting for age, sex, body mass index, diabetes, and dyslipidemia (p < 0.01 for both adjusted models). The associations of BARD with anthropometric measures were significant only in the non-adjusted model. There was a positive association between both FLI and BARD and C-reactive protein. Our results point towards a positive association between waist-to-hip ratio and the risk of hepatic steatosis, and between pro-inflammatory markers and both hepatic steatosis and fibrosis.
Advances in Musculoskeletal Imaging in Juvenile Idiopathic Arthritis
Authors: Sudoł-Szopińska Iwona , Herregods Nele , Doria Andrea S. , Taljanovic Mihra S. , Gietka Piotr , Tzaribachev Nikolay , Klauser Andrea Sabine
Published: September 2022

Over the past decade, imaging of inflammatory arthritis in juvenile arthropathies has significantly advanced due to technological improvements in the imaging modalities and elaboration of imaging recommendations and protocols through systematic international collaboration. This review presents the latest developments in ultrasound (US) and magnetic resonance imaging (MRI) of the peripheral and axial joints in juvenile idiopathic arthritis. In the field of US, the ultra-wideband and ultra-high-frequency transducers provide outstanding spatial resolution. The more sensitive Doppler options further improve the assessment and quantification of the vascularization of inflamed tissues, and shear wave elastography enables the diagnosis of tissue stiffness. Concerning MRI, substantial progress has been achieved due to technological improvements in combination with the development of semiquantitative scoring systems for the assessment of inflammation and the introduction of new definitions addressing the pediatric population. New solutions, such as superb microflow imaging, shear wave elastography, volume-interpolated breath-hold examination, and MRI-based synthetic computed tomography open new diagnostic possibilities and, at the same time, pose new challenges in terms of clinical applications and the interpretation of findings.
Association of Circular RNA and Long Non-Coding RNA Dysregulation with the Clinical Response to Immune Checkpoint Blockade in Cutaneous Metastatic Melanoma
Authors: Oliver Javier , Onieva Juan Luis , Garrido-Barros Maria , Berciano-Guerrero Miguel-Ángel , Sánchez-Muñoz Alfonso , José Lozano María , Farngren Angela , Álvarez Martina , Martínez-Gálvez Beatriz , Pérez-Ruiz Elisabeth , Alba Emilio , Cobo Manuel , Rueda-Domínguez Antonio , Barragán Isabel
Published: September 2022

Cutaneous melanoma (CM) is the most lethal form of skin cancer if it becomes metastatic, where treatment options and survival chances decrease dramatically. Immunotherapy treatments based on the immunologic checkpoint inhibitors programmed death cell protein 1 (PD-1) and cytotoxic T-lymphocyte antigen 4 (CTLA-4) constituted a main breakthrough in the treatment of metastatic CM, particularly for the achievement of long-term benefits. Even though it is a very promising therapy, resistance to primary immune checkpoint blockade (ICB) arises in about 70% of CM patients treated with a CTLA-4 inhibitor, and 40–65% of CM patients administered with a PD-1-targeting treatment. Some long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) are implicated in triggering pro- and anti-tumorigenic responses to various cancer treatments. The relationship between lncRNAs, circRNAs and ICB immunotherapy has not been explored in cutaneous metastatic melanoma (CMM). The aim of this pilot study is to evaluate the potential role of circRNA and lncRNA expression variability as pre-treatment predictor of the clinical response to immunotherapy in CMM patients. RNA-seq from 12 formalin-fixed paraffin-embedded (FFPE) samples from the metastatic biopsies of CMM patients treated with nivolumab was used to identify response-associated transcripts. Our findings indicate that specific lncRNAs and circRNAs, probably acting as competitive endogenous RNAs (ceRNAs), are involved in the regulatory networks of the immune response against metastatic melanoma that these patients have under treatment with nivolumab. Moreover, we established a risk score that yields predictions of the overall survival (OS) and progression-free survival (PFS) of CMM patients with high accuracy. This proof-of-principle work provides a possible insight into the function of ceRNAs, contributing to efforts to decipher the complex molecular mechanisms of ICB cancer treatment response.
Egret Swarm Optimization Algorithm: An Evolutionary Computation Approach for Model Free Optimization
Authors: Chen Zuyan , Francis Adam , Li Shuai , Liao Bolin , Xiao Dunhui , Ha Tran , Li Jianfeng , Ding Lei , Cao Xinwei
Published: September 2022

A novel meta-heuristic algorithm named Egret Swarm Optimization Algorithm (ESOA) is proposed in this paper, which is inspired by two egret species’ hunting behavior (Great Egret and Snowy Egret). ESOA consists of three primary components: a sit-and-wait strategy, aggressive strategy as well as discriminant conditions. The learnable sit-and-wait strategy guides the egret to the most probable solution by applying a pseudo gradient estimator. The aggressive strategy uses random wandering and encirclement mechanisms to allow for optimal solution exploration. The discriminant model is utilized to balance the two strategies. The proposed approach provides a parallel framework and a strategy for parameter learning through historical information that can be adapted to most scenarios and has well stability. The performance of ESOA on 36 benchmark functions as well as 3 engineering problems are compared with Particle Swarm Optimization (PSO), Genetic Algorithm (GA), Differential Evolution (DE), Grey Wolf Optimizer (GWO), and Harris Hawks Optimization (HHO). The result proves the superior effectiveness and robustness of ESOA. ESOA acquires the winner in all unimodal functions and reaches statistic scores all above 9.9, while the scores are better in complex functions as 10.96 and 11.92.
Scientific Research Conformity of University Teachers: Role of Incentives and Internal Attribution
Authors: Song Guandong , Xiao Bin , Wang Sihui
Published: September 2022

Background: Conformity is a process by which a person changes his original idea and attitude in response to group pressure and chooses to be consistent with the majority. The study was undertaken to explore university teachers’ scientific research conformity (SRC), the psychological process of information processing and the types of internal attribution in SRC, and the relationship between SRC incentives and types of internal attribution. Method: A survey of 349 teachers from seven universities was conducted. We employed the mixed-method approach; data was collected through in-depth interviews and were analyzed using AMOS. Results: In-depth interviews revealed that the basic principle of human organizational behavior is conformity with incentive, and conformity is a motivational behavior produced through psychological processing of social information. Factor analysis results revealed that teachers’ SRC is characterized by abidance, compliance, and obedience. The internal incentives mainly affect abidance, and have a significant impact on obedience and compliance; the environmental incentives mainly affect abidance, and have a significant impact on obedience. Family and social relationship incentives mainly affect compliance, but social relationship incentives also have a significant impact on obedience. Additionally, policy incentives have a highly significant impact on obedience. Conclusions: This study provides first-hand empirical data for studies pertaining to research motivation and SRC behavior of Chinese university teachers. It also provides a theoretical basis for subsequent research on conformity behavior.
The Role of Inflammation in Cognitive Impairment of Obstructive Sleep Apnea Syndrome
Authors: Yang Chunlan , Zhou Yuanqing , Liu Haijun , Xu Ping
Published: September 2022

Obstructive sleep apnea syndrome (OSAS) has become a major worldwide public health concern, given its global prevalence. It has clear links with multiple comorbidities and mortality. Cognitive impairment is one related comorbidity causing great pressure on individuals and society. The clinical manifestations of cognitive impairment in OSAS include decline in attention/vigilance, verbal–visual memory loss, visuospatial/structural ability impairment, and executive dysfunction. It has been proven that chronic intermittent hypoxia (CIH) may be a main cause of cognitive impairment in OSAS. Inflammation plays important roles in CIH-induced cognitive dysfunction. Furthermore, the nuclear factor kappa B and hypoxia-inducible factor 1 alpha pathways play significant roles in this inflammatory mechanism. Continuous positive airway pressure is an effective therapy for OSAS; however, its effect on cognitive impairment is suboptimal. Therefore, in this review, we address the role inflammation plays in the development of neuro-impairment in OSAS and the association between OSAS and cognitive impairment to provide an overview of its pathophysiology. We believe that furthering the understanding of the inflammatory mechanisms involved in OSAS-associated cognitive impairment could lead to the development of appropriate and effective therapy.
Exploring the Clinical Utility of the Music Therapy Assessment Tool for Awareness in Disorders of Consciousness (MATADOC) with People with End-Stage Dementia
Authors: Magee Wendy Louise , Lipe Anne Wheeler , Ikeda Takayoshi , Siegert Richard John
Published: September 2022

Dementia is a major health concern globally and cross-culturally with progressive decline in cognition, mobility and communication. There are few interventions for end-stage dementia (ESD) although music interventions have been observed to be accessible for people with mid to late-stage dementia. The lack of protocols and measures suited to ESD has limited research into the effects of music therapy. Measure sensitivity to minimal responsiveness is one limitation to the use of existing music intervention measures with ESD. This exploratory study examined the clinical utility of the Music Therapy Assessment Tool for Awareness in Disorders of Consciousness (MATADOC) for use with people with end-stage dementia, including preliminary reliability and validity. The MATADOC is a standardized assessment for minimally responsive patients with disorders of consciousness and may be useful for ESD. Using repeated measures with blinded MATADOC-trained raters, MATADOC data were collected with a small convenience sample of people with ESD in a residential care setting. Clinical utility data were collected from the raters and evaluated using a multidimensional model. To explore its functionality, MATADOC outcomes were compared to another measure for music interventions in dementia. The MATADOC may be useful for assessing functioning and responsiveness to music interventions for people with ESD without the risk of floor effects. Modifying the MATADOC protocol and assessment documentation prior to testing with a larger sample will enhance its sensitivity specific to ESD and age-related needs, providing a new music-based ESD assessment.
The Therapeutic Potential of Imidazole or Quinone-Based Compounds as Radiosensitisers in Combination with Radiotherapy for the Treatment of Head and Neck Squamous Cell Carcinoma
Authors: Azad Abul , Kong Anthony
Published: September 2022

The addition of platinum chemotherapy to primary radiotherapy (chemoradiation) improves survival outcomes for patients with head and neck squamous cell carcinoma (HNSCC), but it carries a high incidence of acute and long-term treatment-related complications, resulting in a poor quality of life. In addition, patients with significant co-morbidities, or older patients, cannot tolerate or do not benefit from concurrent chemoradiation. These patients are often treated with radiotherapy alone resulting in poor locoregional control and worse survival outcomes. Thus, there is an urgent need to assess other less toxic treatment modalities, which could become an alternative to chemoradiation in HNSCC. Currently, there are several promising anti-cancer drugs available, but there has been very limited success so far in replacing concurrent chemoradiation due to their low efficacy or increased toxicities. However, there is new hope that a treatment strategy that incorporates agents that act as radiosensitisers to improve the efficacy of conventional radiotherapy could be an alternative to more toxic chemotherapeutic agents. Recently, imidazole-based or quinone-based anti-malarial compounds have drawn considerable attention as potential radiosensitisers in several cancers. Here, we will discuss the possibility of using these compounds as radiosensitisers, which could be assessed as safe and effective alternatives to chemotherapy, particularly for patients with HNSCC that are not suitable for concurrent chemotherapy due to their age or co-morbidities or in metastatic settings. In addition, these agents could also be tested to assess their efficacy in combination with immunotherapy in recurrent and metastatic settings or in combination with radiotherapy and immunotherapy in curative settings.
The “Sweet Spot” of Targeting Tumor Metabolism in Ovarian Cancers
Authors: Tondo-Steele Katelyn , McLean Karen
Published: September 2022

The objective of this review is to explore the metabolomic environment of epithelial ovarian cancer that contributes to chemoresistance and to use this knowledge to identify possible targets for therapeutic intervention. The Warburg effect describes increased glucose uptake and lactate production in cancer cells. In ovarian cancer, we require a better understanding of how cancer cells reprogram their glycogen metabolism to overcome their nutrient deficient environment and become chemoresistant. Glucose metabolism in ovarian cancer cells has been proposed to be influenced by altered fatty acid metabolism, oxidative phosphorylation, and acidification of the tumor microenvironment. We investigate several markers of altered metabolism in ovarian cancer including hypoxia-induced factor 1, VEGF, leptin, insulin-like growth factors, and glucose transporters. We also discuss the signaling pathways involved with these biomarkers including PI3K/AKT/mTOR, JAK/STAT and OXPHOS. This review outlines potential metabolic targets to overcome chemoresistance in ovarian cancer. Continued research of the metabolic changes in ovarian cancer is needed to identify and target these alterations to improve treatment approaches.
Oncolytic Adenoviruses: The Cold War against Cancer Finally Turns Hot
Authors: Oronsky Bryan , Gastman Brian , Conley Anthony P. , Reid Christopher , Caroen Scott , Reid Tony
Published: September 2022

Oncolytic viruses, colloquially referred to as “living drugs”, amplify themselves and the therapeutic transgenes that they carry to stimulate an immune response both locally and systemically. Remarkable exceptions aside, such as the recent 14-patient trial with the PD-1 inhibitor, dostarlimab, in mismatch repair (MMR) deficient rectal cancer, where the complete response rate was 100%, checkpoint inhibitors are not cure-alls, which suggests the need for a combination partner like oncolytic viruses to prime and augment their activity. This review focuses on adenoviruses, the most clinically investigated of all the oncolytic viruses. It covers specific design features of clinical adenoviral candidates and highlights their potential both alone and in combination with checkpoint inhibitors in clinical trials to turn immunologically “cold” and unresponsive tumors into “hotter” and more responsive ones through a domino effect. Finally, a “mix-and-match” combination of therapies based on the paradigm of the cancer-immunity cycle is proposed to augment the immune responses of oncolytic adenoviruses.
Identification of Essential Tumor-Infiltrating Immune Cells and Relevant Genes in Left-Sided and Right-Sided Colon Cancers
Authors: Su Chen , Lin Zeyang , Cui Yongmei , Cai Jian-Chun , Hou Jingjing
Published: September 2022

Backgrounds: Colorectal cancer is the third most prevalent cancer worldwide. A right-sided colon cancer patient typically has a worse prognosis than one who has a left-sided colon cancer. There is an unclear understanding of how left-sided colon cancer differs from right-sided colon cancer in tumor-infiltrating immune cells (TIICs) and relevant genes. Methods: The Cancer Genome Atlas provided RNA-seq data and clinical information regarding colon adenocarcinoma. We conducted a single-sample gene set enrichment analysis (ssGSEA) to quantify the level of 24 immune cells infiltrating the tissues. Based on an analysis of univariate Cox regression, immune cell types associated with survival were identified. Weighted gene co-expression network analysis (WGCNA) was used to identify hub genes related to location and critical immune cells. Based on the Search Tool for the Retrieval of Interacting Genes (STRING), interaction potential was predicted among the hub genes. Hub genes that influence outcomes through immune infiltration were identified using the least absolute shrinkage and selection operator (LASSO). Then, we used the TISIDB database (a repository portal for tumor–immune system interactions) to validate the correlation between hub genes and immune cell infiltration. Finally, immunohistochemical assays were conducted to determine the levels of proteins expressed by critical TIICs and cancer cells. Results: Colon cancers on the right side of the body had higher levels of myeloid-derived suppressor cells (MDSCs) than on the left side. There were three key genes: LCP1, ITGB2, and IKZF1. It was found that their expression was linked to poor prognosis and an increased level of MDSC infiltration. An immunohistochemical study confirmed these findings. Conclusions: There is a higher rate of MDSC infiltration in right-sided colon cancer when compared with left-sided colon cancer. COAD outcomes are associated with changes in MDSC infiltration, and therefore LCP1, ITGB2, and IKZF1 may be novel targets for immunotherapy.