bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced Thermoelectric Performance of Na0.55CoO2 Ceramics Doped by Transition and Heavy Metal Oxides
Authors: Krasutskaya Natalie S. , Klyndyuk Andrei I. , Evseeva Lyudmila E. , Gundilovich Nikolai N. , Chizhova Ekaterina A. , Paspelau Andrei V.
Published: May 2024

Using the solid-state reactions method Na0.55(Co,M)O2 (M = Cr, Ni, Zn, W, and Bi) ceramics were prepared and their crystal structure, microstructure, electrophysical, thermophysical, and thermoelectric properties were studied. Doping of Na0.55CoO2 by transition or heavy metal oxides led to the increase in the grain size of ceramics, a decrease in electrical resistivity and thermal diffusivity values, and a sharp increase in the Seebeck coefficient, which resulted in essential enhancement of their thermoelectric properties. The largest power factor (1.04 mW/(m·K2) at 1073 K) and figure of merit (0.702 at 1073 K) among the studied samples possessed the Na0.55Co0.9Bi0.1O2 compound, which also demonstrated the highest values of the Seebeck coefficient (666 μV/K at 1073 K). The obtained results show that the doping of layered sodium cobaltite by different metal oxides allows for improving its stability, microstructure, and functional properties, which proves the effectiveness of the doping strategy for developing new thermoelectric oxides with enhanced thermoelectric performance.
Seasonal Data Cleaning for Sales with Chase Demand Strategy
Authors: Malindzakova Marcela , Malindzak Dušan , Kubik Andrzej
Published: May 2024

The intricate process of planning production, involving product life cycle management and the synthesis of manufacturing information, is crucial for coherence in manufacturing. Manufacturing companies, operating in a high-mix, low-volume production environment, integrate production planning with management to focus on production processes, emphasizing high-quality, rapid product delivery. This includes material item planning to anticipate future demands and ensure sufficient raw material and finished product quantities, considering purchasing, production, and sales capacities. This study explores the electro technical sector, specifically a manufacturing entity specializing in low-voltage plastic cable distribution boxes. It scrutinizes the vital role of seasonal data cleaning in optimizing production planning, with a targeted focus on three products. The implementation of a chase demand strategy is related to capacity planning, taking into account the change in production capacity linked to demand over time. The problem in implementing this strategy is related to the fluctuating level of quality due to changes in demand for specified products.
Application of Calcium Carbonate in the Pharmaceutical Removal Process
Authors: Zielińska Izabela , Polak Daniel , Jurkiewicz Aleksandra , Osełkowska Julia , Lorek Aleksandra , Stor Michał , Krasiński Andrzej , Gierycz Paweł , Szwast Maciej
Published: May 2024

One way to reduce the negative impact of human activity on the natural environment is to use natural, easily available and relatively cheap to produce compounds in industrial processes. One such compound is naturally occurring calcium carbonate (CaCO3). This compound has adsorption properties so that it can be an alternative to commonly used adsorbents. The aim of this work is to determine the possibility of using CaCO3 to remove pharmaceutical substances such as sulfadiazine and tetracycline from water. The CaCO3 used in this work was synthesised using our own method, which allows the production of CaCO3 particles with nanometric size. In the conducted research, calcium carbonate was used in the form of a suspension in purified solutions and as an inorganic filling of the developed membranes. The mass of pharmaceutical substances removed from their aqueous solutions was determined in the tests carried out. Based on the results obtained, it can be concluded that CaCO3 has the ability to adsorb both tetracycline and sulfadiazine. In suspension tests, the mass of the removed substances per unit mass of adsorbent was 1.52 mg/g and 6.85 mg/g, respectively. In turn, in the case of the integrated process using the developed membranes, the mass of the removed substances per unit mass of adsorbent was 109 mg/g and 97 mg/g.
The Influence of Organic Food Purchase Intention on Household Food Waste: Insights from Brazil
Authors: Deliberador Lucas Rodrigues , Santos Alexandre Borges , Queiroz Geandra Alves , César Aldara da Silva , Batalha Mário Otávio
Published: May 2024

An emerging trend among consumers is the pursuit of healthier eating habits while minimizing environmental damage, thus increasing the intention for consumption of organic foods. In this context, this study hypothesizes that household food waste is influenced by organic food purchase intention, which, in turn, is influenced by environmental concern, health consciousness, hedonic shopping, and negatively affected by price. Hence, the objective of this study is to propose and empirically test a theoretical framework with Brazilian consumers. Data from 240 respondents were electronically collected and analyzed through structural equation modeling. The results suggest that environmental concern, price consciousness, and health consciousness significantly influence organic food purchase intention, while hedonic shopping value does not. Furthermore, although organic food purchase intention reduced household food waste, the effect size of this relationship was found to be extremely low, suggesting it to be a poor predictor. This paper contributes to the existing literature on consumer behavior and waste management further by not only examining the relationship between two research streams but also by shedding light on unique aspects of an emerging country.
Assessing the Potential Climate Impacts and Benefits of Waste Prevention and Management: A Case Study of Sweden
Authors: Miliute-Plepiene Jurate , Sundqvist Jan-Olov
Published: May 2024

This study employs a life cycle perspective to analyze the carbon footprints of various waste streams, evaluating 52 cases across 26 types of household waste in Sweden, with a focus on waste prevention and management. It demonstrates that while recycling can reduce carbon emissions, prevention could significantly enhance these benefits, with savings ranging from −36.5 to −0.01 kg-CO2-eq per kg of waste. Notably, Waste Electrical and Electronic Equipment (WEEE), textiles, tires, residual household, and plastic waste are the top five fractions most amenable to prevention on a per mass basis. Further analysis, considering waste volumes, shows that targeted recycling of materials like WEEE, metals, and paper could account for over 80% of potential carbon savings. However, the majority of potential climate impact is attributed to the energy recovery of unsorted (mixed) waste, contributing to more than 90% of total impacts. Redirecting all mixed waste to recycling could triple carbon savings, but focusing on prevention could potentially increase benefits twenty-sevenfold, particularly for waste like WEEE, food, and textiles. This research provides a valuable tool for identifying key areas in waste management to optimize climate benefits and enhance public awareness. However, it advises using local data for precise planning due to inherent uncertainties.
Sustainability Science Communication: Case Study of a True Cost Campaign in Germany
Authors: Stein Lennart , Michalke Amelie , Gaugler Tobias , Stoll-Kleemann Susanne
Published: May 2024

The Anthropocene, marked by human-induced climate change, necessitates urgent action to address climate goals and respect planetary boundaries. While sustainability research provides knowledge, the first challenge lies in communicating the findings in an adequate manner to the public and several stakeholders, such as economic and political actors. Therefore, this study explores the significance of science communication in sustainability science, focusing on a case study—the True Cost Accounting (TCA) campaign by the University of Greifswald, Technical Institute of Nuremberg, and German retailer PENNY. TCA herein serves as a transparency tool, economic incentive, and discussion basis for sustainable consumption. This study investigates consumer perceptions of ecological prices of foods through a face-to-face survey during the 2023 PENNY campaign, comparing results to an informational campaign carried out in 2021. Findings indicate a high awareness of the true cost campaign in 2023, with 50.8% of participants hearing about it. Consumers’ willingness to pay true costs and potential behavior changes were explored. In comparison to results from the informational campaign of 2021, customers showed a decrease in this WTP when the true prices would actually impact their spending, indicating an attitude–behavior gap. In addition, a willingness to reduce the consumption of animal foods—if TCA was implemented—of 60.5% was determined, which suggests that TCA has the potential for sustainable behavior change. This study highlights factors that influence consumer attitudes and preferences regarding the inclusion of TCAs, such as environmental, social, and animal welfare costs. Customers’ understanding of increased prices—like, in this case, the compensation for environmental and social costs—is an argument in favor of true prices. The results emphasize the need for differentiated scientific communication strategies to bridge knowledge and action gaps in sustainability science.
The Effect of Varying Olive Mill Wastewater Concentrations on Soil Free-Living Nematode Communities and Lettuce Growth
Authors: Kekelis Panagiotis , Pantazi Cleopatra , Mourouzidou Snezhana , Theofilidou Aphrodite , Dimou Maria D. , Aschonitis Vassilis , Monokrousos Nikolaos
Published: May 2024

We assessed the impact of increasing olive mill waste (OMW) concentrations (10%, 35%, 70%, and 100% v/v) on soil free-living nematode communities and Lactuca sativa (lettuce) growth, 10 and 45 days after application (DAA). L. sativa plants showed a survival threshold at OMW10%, with higher concentrations proving fatal. Contrary to expectations, nematode abundance increased with OMW concentration. OMW10% induced a rapid surge in nematode abundance, stabilizing at 45 DAA, resembling control values. OMW35%, OMW70%, and OMW100% plots exhibited persistent, gradual increases, surpassing control values at 45 DAA. All treatments favored fungal feeders, resulting in the overdominance of the genus Aphelenchus both at 10 and 45 DAA. Even though OMW did not exert a toxic effect on nematode populations, this shift in the community structure towards the dominance of a single genus could suggest an imbalance in the soil community, which could have negative implications for soil health and ecosystem functioning. Overall, our study provides insights into the complex interactions between OMW, soil nematode communities, and plant growth, emphasizing the importance of understanding soil ecology for sustainable agricultural management.
Impact of Environmental Regulatory Types and Green Technological Innovation on Green Total Factor Productivity in Polluted Areas of China
Authors: Sun Mingzhe , Zhao Lingdi
Published: May 2024

Compared to regions with lighter pollution, the areas heavily affected by pollution in China face more severe environmental problems due to rapid economic growth, which creates a greater urgency for government and corporate environmental requirements. This study innovatively applies mediation and threshold models to explore the potential correlation between green technology innovation, types of environmental regulation, and provincial-level green total factor productivity (GTFP). Additionally, it examines inter-regional differences, determines threshold effects, and introduces regional heterogeneity and mediator variables. The research findings demonstrate that progress in green technology innovation significantly impacts the improvement of provincial-level GTFP. Further mechanism analysis reveals the crucial role of environmental regulation in facilitating sustained enhancement of GTFP through green technology innovation. The promotion of GTFP is more pronounced in eastern and central provinces compared to western regions, and the positive influence of green technology innovation varies significantly among provinces. By investigating the regional differences of polluted areas and introducing mediating variables, this paper explores the environmental regulation mechanism, which has important guiding significance for formulating more effective environmental regulation policies, promoting green technology innovation, and improving GTFP.
Water Banking as a Strategy for the Management and Conservation of a Critical Resource: A Case Study from Tunisia’s Medjerda River Basin (MRB)
Authors: Sawassi Aymen , Khadra Roula , Crookston Brian
Published: May 2024

The increasingly adverse impacts of climate change (e.g., rainfall patterns, droughts, and floods), coupled with the ever-increasing water demands, are often translated into a contingent liability for water users’ communities. Additional complexities arise due to competing priorities, water rights, and transboundary water sources. Therefore, conventional water management practices should shift toward more comprehensive and responsive integrative approaches, even for systems with limited data. Furthermore, water managers must prioritize dynamic and interactive management techniques for existing systems. One such management technique is water banking, which is the focus of this study. Herein, a dynamic interactive water allocation model, which encompasses the water managers and heterogeneous parties with competing demands, is developed. The voluntary sales of water shares between parties are illustrated through the specific case of the Medjerda River in Tunisia, an excellent example of a transboundary basin with limited hydrologic data and conflicting water use requirements between its upstream and downstream sectors. A set of scenarios is developed for the first analysis with this model: two management scenarios that include the no-water trade and the water banking option; three demand scenarios that include a combination of steady-, low-, and high-water demand conditions; and two hydrologic scenarios that include dry and wet conditions. Based on an economic model, the economic impacts of water banking are calculated using estimates of the costs of water shortages brought to users that illustrate the magnitude. The results show that the water banking technique can improve water resource availability by optimizing the management, operation, and conservation of natural and artificial water storage systems and water distribution infrastructure. Specifically, water banking can offset users’ profit losses during severe conditions (i.e., drought), even with limited hydrologic data. This water management technique would allow the Tunisian government to minimize the economic impacts on farmers from drought and to plan for future uncertainties by optimizing the water storage potential in years of abundant rainfall.
How Credible Is the 25-Year Photovoltaic (PV) Performance Warranty?—A Techno-Financial Evaluation and Implications for the Sustainable Development of the PV Industry
Authors: Hsi Pao-Hsiang , Shieh Joseph C. P.
Published: May 2024

To support the bankability of PV projects, PV manufacturers have been offering one of the longest warranties in the world, typically in the range of 25–30 years. During the warranty period, PV manufacturers guarantee that the degradation of PV modules will not exceed 0.4–0.6% each year, or the buyer can at any time make a claim to the manufacturer for replacement or compensation for the shortfall. Due to its popularity, the performance warranty terms have become more and more competitive each year. However, long-term PV operating data have been very limited and bankruptcy of PV manufacturers has been quite common. Without a proper methodology to assess the adequacy of PV manufacturer’s warranty fund (WF) reserve, the 25-year performance warranty can become empty promises. To ensure sustainable development of the PV industry, this study develops a probability-weighted expected value method to determine the necessary WF reserve based on benchmark field degradation data and prevailing degradation cap of 0.55% per year. The simulation result shows that, unless the manufacturer’s degradation pattern is significantly better than the benchmark degradation profile, 1.302% of the sales value is required for the WF reserve. To the best of our knowledge, this is the first study that provides WF reserve requirement estimation for 25-year PV performance warranty. The result will provide transparency for PV investors and motivation for PV manufacturers for continuous quality improvement as all such achievement can now be reflected in manufacturers’ annual report result.
On Multiple-Type Wave Solutions for the Nonlinear Coupled Time-Fractional Schrödinger Model
Authors: Mohammed Pshtiwan Othman , Agarwal Ravi P. , Brevik Iver , Abdelwahed Mohamed , Kashuri Artion , Yousif Majeed A.
Published: May 2024

Recently, nonlinear fractional models have become increasingly important for describing phenomena occurring in science and engineering fields, especially those including symmetric kernels. In the current article, we examine two reliable methods for solving fractional coupled nonlinear Schrödinger models. These methods are known as the Sardar-subequation technique (SSET) and the improved generalized tanh-function technique (IGTHFT). Numerous novel soliton solutions are computed using different formats, such as periodic, bell-shaped, dark, and combination single bright along with kink, periodic, and single soliton solutions. Additionally, single solitary wave, multi-wave, and periodic kink combined solutions are evaluated. The behavioral traits of the retrieved solutions are illustrated by certain distinctive two-dimensional, three-dimensional, and contour graphs. The results are encouraging, since they show that the suggested methods are trustworthy, consistent, and efficient in finding accurate solutions to the various challenging nonlinear problems that have recently surfaced in applied sciences, engineering, and nonlinear optics.
Construction of an Elastin-like Polypeptide Gene in a High Copy Number Plasmid Using a Modified Method of Recursive Directional Ligation
Authors: Nelson Derek W. , Connor Alexander , Shen Yu , Gilbert Ryan J.
Published: May 2024

Elastin-like polypeptides (ELPs) are popular biomaterials due to their reversible, temperature-dependent phase separation and their tunability, which is achievable by evolving procedures in recombinant technology. In particular, recursive direction ligation by plasmid reconstruction (PRe-RDL) is the predominant cloning technique used to generate ELPs of varying lengths. Pre-RDL provides precise control over the number of (VPGXG)n repeat units in an ELP due to the selection of type IIS restriction enzyme (REs) sites in the reconstructed pET expression plasmid, which is a low-to-medium copy number plasmid. While Pre-RDL can be used to seamlessly repeat essentially any gene sequence and overcome limitations of previous cloning practices, we modified the Pre-RDL technique, where a high copy number plasmid (pBluescript II SK(+)—using a new library of type IIS REs) was used instead of a pET plasmid. The modified technique successfully produced a diblock ELP gene of 240 pentapeptide repeats from 30 pentapeptide “monomers” composed of alanine, tyrosine, and leucine X residues. This study found that the large, GC-rich ELP gene compromised plasmid yields in pBluescript II SK(+) and favored higher plasmid yields in the pET19b expression plasmid. Additionally, the BL21 E. coli strain expression consistently provided a higher transformation efficiency and higher plasmid yield than the high cloning efficiency strain TOP10 E. coli. We hypothesize that the plasmid/high GC gene ratio may play a significant role in these observations, and not the total plasmid size or the total plasmid GC content. While expression of the final gene resulted in a diblock ELP with a phase separation temperature of 34.5 °C, future work will need to investigate RDL techniques in additional plasmids to understand the primary driving factors for improving yields of plasmids with large ELP-encoding genes.
Enhancing Bi2Te2.70Se0.30 Thermoelectric Module Performance through COMSOL Simulations
Authors: Hasan Md. Kamrul , Üstüner Mehmet Ali , Mamur Hayati , Bhuiyan Mohammad Ruhul Amin
Published: May 2024

This research employs the COMSOL Multiphysics software (COMSOL 6.2) to conduct rigorous simulations and assess the performance of a thermoelectric module (TEM) meticulously crafted with alumina (Al2O3), copper (Cu), and Bi2Te2.70Se0.30 thermoelectric (TE) materials. The specific focus is on evaluating diverse aspects of the Bi2Te2.70Se0.30 thermoelectric generator (TEG). The TEM design incorporates Bi2Te2.70Se0.30 for TE legs of the p- and n-type positioned among the Cu layers, Cu as the electrical conductor, and Al2O3 serving as an electrical insulator between the top and bottom layers. A thorough investigation is conducted into critical parameters within the TEM, which include arc length, electric potential, normalized current density, temperature gradient, total heat source, and total net energy rate. The geometric configuration of the square-shaped Bi2Te2.70Se0.30 TEM, measuring 1 mm × 1 mm × 2.5 mm with a 0.25 mm Al2O3 thickness and a 0.125 mm Cu thickness, is scrutinized. This study delves into the transport phenomena of TE devices, exploring the impacts of the Seebeck coefficient (S), thermal conductivity (k), and electrical conductivity (σ) on the temperature differential across the leg geometry. Modeling studies underscore the substantial influence of S = ±2.41 × 10−3 V/K, revealing improved thermal conductivity and decreased electrical conductivity at lower temperatures. The findings highlight the Bi2Te2.70Se0.30 TEM’s high potential for TEG applications, offering valuable insights into design and performance considerations crucial for advancing TE technology.
Independent and Joint Effects of Prenatal Incense-Burning Smoke Exposure and Children’s Early Outdoor Activity on Preschoolers’ Obesity
Authors: Chen Meimei , Strodl Esben , Yang Weikang , Yin Xiaona , Wen Guomin , Sun Dengli , Xian Danxia , Zhao Yafen , Chen Weiqing
Published: May 2024

Incense burning is a significant source of indoor air pollution in many Asian regions. There is emerging evidence that maternal prenatal exposure to incense-burning smoke may be a risk factor for childhood obesity. We aimed to extend this new line of research by investigating the independent and joint effect of incense-burning smoke exposure, and children’s outdoor activity in early life, on preschoolers’ obesity. A total of 69,637 mother–child dyads were recruited from all kindergartens in the Longhua District of Shenzhen, China. Information on sociodemographic characteristics, maternal exposure to incense-burning smoke (IBS) during pregnancy, and frequency and duration of outdoor activity at the age of 1–3 years was collected by a self-administered questionnaire. In addition, the heights and weights of the children were measured by the research team. Logistic regression models and cross-over analyses were conducted to investigate the independent and combined effects of maternal exposure to incense-burning smoke during pregnancy and children’s early outdoor activity on obesity in preschoolers. We found that prenatal exposure to incense-burning smoke increased the risk of the presence of obesity in preschoolers’ (AOR = 1.13, 95% CI = 1.03–1.23). Additionally, lower frequencies (<3 times/week) or shorter durations (<60 min/time) of outdoor activity from the age of 1–3 years were significantly associated with the presence of obesity, with AORs of 1.24 (95% CI =1.18–1.32) and 1.11 (95% CI = 1.05–1.17), respectively. Furthermore, the cross-over analysis showed that prenatal exposure to IBS combined with a lower frequency of early outdoor activity (AOR = 1.47, 95% CI = 1.31–1.66) or a shorter duration of outdoor activity during ages of 1–3 years (AOR = 1.22, 95% CI = 1.07–1.39) increased the risk of obesity in preschoolers. Finally, additive interactions between prenatal exposure to IBS and postnatal outdoor activity on obesity were identified. Our study indicates that maternal exposure to incense-burning smoke during pregnancy and early lower postanal outdoor activity may independently and jointly increase the risk of obesity among preschoolers.
Identification and Visualization of Polystyrene Microplastics/Nanoplastics in Flavored Yogurt by Raman Imaging
Authors: Ling Xin , Cheng Jun , Yao Weirong , Qian He , Ding Dazhi , Yu Zhilong , Xie Yunfei , Yang Fangwei
Published: May 2024

The contamination of food by microplastics has garnered widespread attention, particularly concerning the health risks associated with small-sized microplastics. However, detecting these smaller microplastics in food poses challenges attributed to the complexity of food matrices and instrumental and method limitations. Here, we employed Raman imaging for visualization and identification of polystyrene particles synthesized in polymerization reactions, ranging from 400 to 2600 nm. We successfully developed a quantitative model of particle size and concentration for polystyrene, exhibiting excellent fit (R2 of 0.9946). We established procedures for spiked flavored yogurt using synthesized polystyrene, providing fresh insights into microplastic extraction efficiency. Recovery rates calculated from models validated the method’s feasibility. In practical applications, the assessment of the size, type, shape, and quantity of microplastics in unspiked flavored yogurt was conducted. The most common polymers found were polystyrene, polypropylene, and polyethylene, with the smallest polystyrene sizes ranging from 1 to 10 μm. Additionally, we conducted exposure assessments of microplastics in branded flavored yogurt. This study established a foundation for developing a universal method to quantify microplastics in food, covering synthesis of standards, method development, validation, and application.
Exploring Viral Genome Profile in Mpox Patients during the 2022 Outbreak, in a North-Eastern Centre of Italy
Authors: Deiana Michela , Lavezzari Denise , Mori Antonio , Accordini Silvia , Pomari Elena , Piubelli Chiara , Malagò Simone , Cordioli Maddalena , Ronzoni Niccolò , Angheben Andrea , Tacconelli Evelina , Capobianchi Maria Rosaria , Gobbi Federico Giovanni , Castilletti Concetta
Published: May 2024

In 2022, an unprecedented outbreak of mpox raged in several nations. Sequences from the 2022 outbreak reveal a higher nucleotide substitution if compared with the estimated rate for orthopoxviruses. Recently, intra-lesion SNVs (single nucleotide variants) have been described, and these have been suggested as possible sources of genetic variation. Until now, it has not been clear if the presence of several SNVs could represents the result of local mutagenesis or a possible co-infection. We investigated the significance of SNVs through whole-genome sequencing analysis of four unrelated mpox cases. In addition to the known mutations harboured by the circulating strains of virus (MPXV), 7 novel mutations were identified, including SNVs located in genes that are involved in immune evasion mechanisms and/or viral fitness, six of these appeared to be APOBEC3-driven. Interestingly, three patients exhibited the coexistence of mutated and wild-type alleles for five non-synonymous variants. In addition, two patients, apparently unrelated, showed an analogous pattern for two novel mutations, albeit with divergent frequencies. The coexistence of mixed viral populations, harbouring non-synonymous mutations in patients, supports the hypothesis of possible co-infection. Additional investigations of larger clinical cohorts are essential to validating intra-patient viral genome heterogeneity and determining the possibility of co-presence events of slightly divergent MPXV strains.
Regional Variation of the CD4 and CD8 T Cell Epitopes Conserved in Circulating Dengue Viruses and Shared with Potential Vaccine Candidates
Authors: Chawla Yadya M. , Bajpai Prashant , Saini Keshav , Reddy Elluri Seetharami , Patel Ashok Kumar , Murali-Krishna Kaja , Chandele Anmol
Published: May 2024

As dengue expands globally and many vaccines are under trials, there is a growing recognition of the need for assessing T cell immunity in addition to assessing the functions of neutralizing antibodies during these endeavors. While several dengue-specific experimentally validated T cell epitopes are known, less is understood about which of these epitopes are conserved among circulating dengue viruses and also shared by potential vaccine candidates. As India emerges as the epicenter of the dengue disease burden and vaccine trials commence in this region, we have here aligned known dengue specific T cell epitopes, reported from other parts of the world with published polyprotein sequences of 107 dengue virus isolates available from India. Of the 1305 CD4 and 584 CD8 epitopes, we found that 24% and 41%, respectively, were conserved universally, whereas 27% and 13% were absent in any viral isolates. With these data, we catalogued epitopes conserved in circulating dengue viruses from India and matched them with each of the six vaccine candidates under consideration (TV003, TDEN, DPIV, CYD-TDV, DENVax and TVDV). Similar analyses with viruses from Thailand, Brazil and Mexico revealed regional overlaps and variations in these patterns. Thus, our study provides detailed and nuanced insights into regional variation that should be considered for itemization of T cell responses during dengue natural infection and vaccine design, testing and evaluation.
Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins
Authors: Kulanayake Shermila , Dar Faryal , Tikoo Suresh
Published: May 2024

The L 1 region of bovine adenovirus (BAdV)-3 encodes a multifunctional protein named protein VII. Anti-protein VII sera detected a protein of 26 kDa in transfected or BAdV-3-infected cells, which localizes to nucleus and nucleolus of infected/transfected cells. Analysis of mutant protein VII identified four redundant overlapping nuclear/nucleolar localization signals as deletion of all four potential nuclear/nucleolar localization signals localizes protein VII predominantly to the cytoplasm. The nuclear import of protein VII appears to use importin α (α-1), importin-β (β-1) and transportin-3 nuclear transport receptors. In addition, different nuclear transport receptors also require part of protein VII outside nuclear localization sequences for efficient interaction. Proteomic analysis of protein complexes purified from recombinant BAdV-3 expressing protein VII containing Strep Tag II identified potential viral and cellular proteins interacting with protein VII. Here, we confirm that protein VII interacts with IVa2 and protein VIII in BAdV-3-infected cells. Moreover, amino acids 91–101 and 126–137, parts of non-conserved region of protein VII, are required for interaction with IVa2 and protein VIII, respectively.
Anti-SARS-CoV-2 Antibodies Level and COVID-19 Vaccine Boosters among Healthcare Workers with the Highest SARS-CoV-2 Infection Risk—Follow Up Study
Authors: Lorent Dagny , Nowak Rafał , Figlerowicz Magdalena , Handschuh Luiza , Zmora Paweł
Published: May 2024

During the COVID-19 pandemic, several vaccines were developed to limit the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, due to SARS-CoV-2 mutations and uneven vaccination coverage among populations, a series of COVID-19 waves have been caused by different variants of concern (VOCs). Despite the updated vaccine formulations for the new VOC, the benefits of additional COVID-19 vaccine doses have raised many doubts, even among high-risk groups such as healthcare workers (HCWs). We examined the factors underlying hesitancy to receive COVID-19 booster vaccine doses and analysed the anti-SARS-CoV-2 IgG antibody response after booster vaccination among HCWs. Our study found that 42% of the HCWs were hesitant about the second booster dose, while 7% reported no intent to get vaccinated with any additional doses. As reasons for not vaccinating, participants most frequently highlighted lack of time, negative experiences with previous vaccinations, and immunity conferred by past infections. In addition, we found the lowest post-vaccination antibody titres among HCWs who did not receive any vaccine booster dose and the highest among HCWs vaccinated with two booster doses.
Serological Correlates of Protection Induced by COVID-19 Vaccination in the Working Age Population: A Systematic Review and Meta-Analysis
Authors: Rahmani Alborz , Montecucco Alfredo , Priano Luca , Mandolini Lucia , Dini Guglielmo , Durando Paolo
Published: May 2024

COVID-19 vaccines represent effective public health measures in contrasting the pandemic worldwide. However, protection at the individual-level, which is of crucial importance from an occupational health perspective, is commonly assessed by a serological correlate of protection (CoP) for SARS-CoV-2, which has not yet been determined. The emergence of variants of concern (VOCs) that have shown high rates of breakthrough infections has further complicated the understanding of immune protection against infection. To define a potential serological correlate of protection induced by the COVID-19 vaccination, a systematic review and meta-analysis was performed to summarize the evidence concerning the binding antibody concentration corresponding to a protective effect. Eighteen and four studies were included in the qualitative and quantitative analyses, respectively. The protection against infection was shown for anti-receptor-binding domain (RBD) titers ranging from 154 to 168.2 binding antibody units (BAU)/mL during the pre-Omicron period, while ranging from 1235 to 3035 BAU/mL in the Omicron period. Pooling the results from the studies concerning anti-RBD and anti-Spike antibody titer, we found a mean of 1341.5 BAU/mL and 1400.1 BAU/mL, respectively. These findings suggest that although a fixed serological threshold corresponding to protection against different SARS-CoV-2 variants is not yet definable, higher binding antibody concentrations are associated with increased protective effects.
The Individual Drive of a Wheelset and the Problematics of Its Electromechanical Phenomena
Authors: Dybala Vojtěch , Fridrichovský Tomáš , Kalivoda Jan
Published: May 2024

This paper deals with the phenomenon of torsion oscillations in railway vehicle drive systems. The main goal is to reduce the risk of presence of torsional oscillations in wheelset drive, eventually to propose systems to effectively identify and eliminate torsional oscillations of wheelsets. Therefore, a simulation wheelset drive model including a detailed model of the asynchronous traction motor control was built. The results of computer simulations show that the torsional oscillations can be effectively eliminated by avoiding the resonance states between the excitation frequencies given by pulse width modulation of the inverter and the eigenfrequencies of the mechanical part of the drive. Furthermore, it was found that the presence of torsional oscillations of the wheels can be detected based on the traction motor current ripple. The wheelset drive model was subsequently implemented in a simulation model of a four-axle locomotive. A new algorithm of an anti-slip protection system that utilizes motor currents was implemented in the model. Simulations show that such an anti-slip protection system can prevent the occurrence of undesired large amplitude of wheelset torsional oscillations. The models and simulation results are presented in detail in the paper.
The Establishment of a Novel γ-Interferon In Vitro Release Assay for the Differentiation of Mycobacterial Bovis-Infected and BCG-Vaccinated Cattle
Authors: Zhao Yuhao , Fei Wentao , Yang Li , Xiang Zhijie , Chen Xi , Chen Yingyu , Hu Changmin , Chen Jianguo , Guo Aizhen
Published: May 2024

BCG vaccination is increasingly reconsidered in the effective prevention of bovine tuberculosis (bTB). However, the primary challenge in BCG vaccination for cattle is the lack of a technique for differentiating between infected and vaccinated animals (DIVA). This study aimed to establish a novel DIVA diagnostic test based on an interferon-gamma in vitro release assay (IGRA). The plasmid encoding three differential antigens (Rv3872, CFP-10, and ESAT-6) absent in BCG genes but present in virulent M. bovis was previously constructed. Thus, a recombinant protein called RCE (Rv3872, CFP-10, and ESAT-6) was expressed, and an RCE-based DIVA IGRA (RCE-IGRA) was established. The RCE concentration was optimized at 4 μg/mL by evaluating 97 cattle (74 of which were bTB-positive, and 23 were negative) using a commercial IGRA bTB diagnostic kit. Further, 84 cattle were tested in parallel with the RCE-IGRA and commercial PPD-based IGRA (PPD-IGRA), and the results showed a high correlation with a kappa value of 0.83. The study included BCG-vaccinated calves (n = 6), bTB-positive cattle (n = 6), and bTB-negative non-vaccinated calves (n = 6). After 3 months post-vaccination, PPD-IGRA generated positive results in both vaccinated and infected calves. However, RCE-IGRA developed positive results in infected calves but negative results in vaccinated calves. In conclusion, this DIVA method has broad prospects in differentiating BCG vaccination from natural infection to prevent bTB.
Catchment-Scale Hydrologic Effectiveness of Residential Rain Gardens: A Case Study in Columbia, Maryland, USA
Authors: Daniels Benjamin J. , Yeakley Jon Alan
Published: May 2024

To mitigate the adverse impacts of urban stormwater on streams, watershed managers are increasingly using low-impact development and green infrastructure (LID-GI) stormwater control measures, such as rain gardens—vegetated depressional areas that collect and infiltrate runoff from rooftops and driveways. Their catchment-scale performance, however, can vary widely, and few studies have investigated the cumulative performance of residential rain gardens for event runoff control in intermediate-sized (i.e., 1–10 km2) suburban catchments. We modeled three years of continuous rainfall-runoff from a 3.1 km2 catchment in Columbia, MD, USA, using the Storm Water Management Model (SWMM). Various extents of rain garden implementation at residential houses were simulated (i.e., 25%, 50%, 75%, and 100% coverage) to determine the effects on peak flow, runoff volume, and lag time. On average, treating 100% of residential rooftops in the catchment reduced peak flows by 14.3%, reduced runoff volumes by 11.4%, and increased lag times by 3.2% for the 223 rainfall events during the simulation period. Peak flow reductions were greater for smaller storm events (p < 0.01). Our results show that residential rain gardens can significantly improve the runoff response of suburban catchments, and that they represent an effective and relatively low-cost option for urban watershed management and restoration.
Applicability of Single-Borehole Dilution Tests in Aquifers with Vertical Flow
Authors: Calvache Maria L. , López-Chicano Manuel , Blanco-Coronas Angela M. , de la Torre Beatriz , Duque Carlos
Published: May 2024

A set of experimental field single-borehole dilution tests were completed in the Motril–Salobreña detrital aquifer (Spain) in a sector with coarse material in four different moments under variable hydrological conditions. The comparative study of the tracer washing, and the temperature profile patterns for the tests carried out in two wells located hundreds of m from each other, revealed the presence of ascending vertical flows in one of the wells (not detected by other means) that compromises the reliability of the tracer test. The values of both the apparent horizontal velocity and hydraulic conductivity obtained in the affected well were less than half of those estimated in the well not affected by the upward vertical flows. The repetition of the test eight times during different seasons showed that the hydraulic conductivity calculated from the apparent horizontal velocity can vary; therefore, to approximate to a representative hydraulic conductivity value, using this method is recommended to carry out tests under different hydrological conditions and average the results. The difference generated by the changes in conditions for the specific setting of the study area was 25%. Taking this into account, it was considered that an approximation to the more representative value would be an average under variable hydrological conditions, resulting in a horizontal velocity of 6.7 m/d and hydraulic conductivity of 337 m/d. This information is critical for the management of the aquifer as it has strategic resources against droughts that are becoming more frequent in the Mediterranean area.
Effects of Different Reclamation Years and Modes on Soil Moisture Transport Pathways and Permeability Characteristics in an Open-Pit Mining Area in Guangxi
Authors: Wang Song , Gan Lei , Zhang Yu , Gao Zhibo , Luo Zhenhong , Zhou Haojie , Zhang Hang , Zhang Hongxia , Huang Taiqing
Published: May 2024

The microstructural characteristics of reclaimed soil in Guangxi’s bauxite mining area play a pivotal role in determining soil reclamation quality, yet they remain poorly understood. To explore the impact of varying reclamation years and modes on pore structure characteristics in this region, we selected four reclamation regions: grassland reclamation with 2 years (RG2a), grassland reclamation with 10 years (RG10a), bare ground reclamation with 2 years (RW2a) and bare ground reclamation with 10 years (RW10a). Utilizing X-ray CT technology, we scanned soil columns within a 30 cm depth to analyze pore distributions using Avizo 2020 software combined with ImageJ 1.53c and its plugins. The findings revealed a significant increase in the number of 2D and 3D macropores by 1.09% and 88.89% in RG10a compared to RG2a, as well as 39.01% and 13.33% in RG10a compared to RW10a, respectively. Furthermore, RG10a was observed to be more effective in enhancing the rounding rate of macropores and mesopores, as well as average branch length and density. Additionally, RG10a demonstrated a greater capacity to increase porosity and connectivity while reducing curvature and specific surface area among the three-dimensional parameters. The 3D reconstruction illustrated that RG modes exhibited a more abundant distribution of macropores compared to RW modes and 10a modes showed a higher presence of macropores than 2a modes, which displayed a tilted laminar pattern. Soil moisture movement simulations conducted through Avizo 2020 under various modes indicated that RG modes presented a more extensive capacity for vertical flow, while 10a modes displayed more abundant flow lines and a higher infiltration rate (K) compared to 2a modes. In conclusion, prolonged reclamation years favoring grassland reclamation modes could enhance water and nutrient transport pathways toward traditional agricultural soil.
Flexible Charging to Energy Saving—Strategies Assessment with Big Data Analysis for PHEVs Private Cars
Authors: Andrenacci Natascia , Giuli Giancarlo , Genovese Antonino , Pede Giovanni
Published: May 2024

In road transport, most vehicles today still rely on internal combustion engines. However, these engines have lower efficiency and generate higher pollution levels compared to electric motors. Consequently, there is a growing interest in the transition from conventional vehicles to electric ones. However, the transition to an electrified road transport system is not without challenges. Among these, the impact that electric vehicle charging will have on the electricity grid is of particular concern. This paper analyzes different charging scenarios for plug-in hybrid electric vehicles (PHEVs) and proposes charging strategies to minimize their impact on the electricity grid. The analysis is based on a large dataset of trips in urban areas in Italy. The study shows that smart charging of PHEVs can be implemented to minimize the impact on the electricity grid. The implementation of optimized charging strategies can contribute to making PHEVs a valid, eco-sustainable alternative to conventional vehicles while also promoting the stability and efficiency of the electricity grid. The study aims to verify the effectiveness and efficiency of the flexible charging strategy by comparing the common charging operation (first in–first out) with other, less impactful charging schemes.
Direct Torque Control of Dual Three-Phase Permanent Magnet Synchronous Motors Based on Master–Slave Virtual Vectors
Authors: Geng Qiang , Qin Ziteng , Jin Xuefeng , Zhang Guozheng , Zhou Zhanqing
Published: May 2024

In order to further reduce the torque, flux-linkage fluctuation, and current harmonic content of dual three-phase permanent magnet synchronous motors, this paper proposes a direct torque control strategy combined with a master–slave virtual vector duty cycle assignment. Two types of virtual voltage vectors with different amplitudes are used to form a harmonic suppression switching table. The virtual vectors are classified into master and slave virtual vectors according to the degree of influence on the torque and the flux-linkage. Then, the duty cycle of the master and slave virtual vectors is recalculated and allocated through the evaluation function to achieve accurate control of the torque and the flux-linkage. Finally, the switching sequences of the master and slave virtual vectors that act together in one control cycle are rearranged into a symmetrical waveform. It is experimentally verified that the phase current THD of the proposed strategy is reduced by 69.4%, the 5th and 7th current harmonics content is significantly reduced, and the torque fluctuation and flux-linkage fluctuation can also be effectively suppressed, which provides better dynamic performance and steady-state performance.
Improved Equivalent Strain Method for Fatigue Life of Automobile Aluminum Alloy
Authors: Zhi Shanjie , Liu Hejian , Liu Xintian
Published: May 2024

Automotive parts are usually subjected to random loads with large mean tensile/compressive stresses under working conditions. It is important for automotive parts to have a long fatigue life under mean stress in practical engineering applications. An equivalent strain model is established here to predict fatigue life considering the influence of mean strain and stress under asymmetric cycles. To predict the fatigue life more accurately, the coefficient of surface roughness and temperature correction is introduced in this model. The effectiveness of the improved equivalent strain (IES) model is verified by comparing it with multiple sets of experimental data. The IES is also compared with Smith–Watson–Topper (SWT), Manson–Coffin, and equivalent strain models. The results show that the developed model has a higher prediction accuracy than the other models. An improved fatigue strength exponent is introduced to modify the equivalent strain model, and the effectiveness of the model is verified by experimental data. The IES model demonstrates significantly reduced standard deviations under various strain ratios (−0.06, 0.06, 0.5), with measurements of 0.0936, 0.0721, and 0.0636, respectively. The method provides a certain reference for the life prediction of automotive parts.