bluuebunny/crossref_metadata_2025 huggingface dataset:
Sugarcane Mosaic
Authors: Rott Philippe , Comstock Jack C. , Gilbert Robert A. , Sandhu Hardev
Published: October 2022

Mosaic’s most distinctive symptom is a pattern of contrasting shades of green, often islands of normal green on a background of paler green or yellowish chlorotic areas on the leaf blade. It had not been a problem in Florida until 1996, when it was observed on CP72-2086, a major commercial cultivar, near the intersection of Hatton Highway and US 98. Presently, because of the limited acreage of CP72-2086, the disease is only a potential threat. This revised 3-page fact sheet was written by P. Rott, J. C. Comstock, R. A. Gilbert, and H. S. Sandhu, and published by the UF Department of Agronomy, March 2015. (Photo: Philippe Rott, UF/IFAS)
Effects of Harvest Method on Microclimate in Florida Sugarcane
Authors: Sandhu Hardev , Singh Maninder , Gilbert Robert , Morgan Kelly , Rice Ronald , Baucum Leslie , Shine James , Irey Mike
Published: October 2022

The production systems for sugarcane include either green cane or burnt cane harvesting operations. Sugarcane in Florida is typically harvested with burnt cane mechanical harvesting, but there is a growing interest to better understand the effects of green cane harvest residue “trash blankets” on microclimate conditions for sugarcane growing on both organic and mineral soils of Florida, so the authors conducted a three-year study to determine the effects of each harvest method on microclimate within the surface soil profile and at a 10 cm height from the soil surface. Results are presented in this 3-page fact sheet written by Hardev Sandhu, Maninder Singh, Robert Gilbert, Kelly Morgan, Ronald Rice, Leslie Baucum, James Shine Jr., and Mike Irey, and published by the UF Department of Agronomy, April 2015. (UF/IFAS Photo: Josh Wickham.) 
SS-AGR-385/SC100: Effects of Harvest Method on Microclimate in Florida Sugarcane (ufl.edu)
Understanding Nitrogen Availability from Applications of Anaerobically Digested Beef-Cattle Manure in Florida Sandy Soil
Authors: Prasad Rishi , Hochmuth George
Published: October 2022

Anaerobic digestion of livestock manure is a microbe-mediated process carried out in vessels or tanks, where the livestock wastes are digested slowly in environment absent of oxygen. The main products are biogas, anaerobically digested liquid (ADL), and solid (ADS), which can be land-applied as an organic soil amendment or a source of plant nutrients. This 4-page fact sheet provides research-based information about using anaerobically digested beef-cattle manure as an organic source of nitrogen for supplementing crop nutrition in Florida sandy soils, including initial N concentration, application timing, rate of application, and method of application. Written by Rishi Prasad and George Hochmuth, and published by the UF Department of Soil and Water Science, April 2015. (Photo: George Hochmuth, UF/IFAS)

SL424/SS637: Understanding Nitrogen Availability from Applications of Anaerobically Digested Beef-Cattle Manure in Florida Sandy Soil (ufl.edu)
Invasion Curve: A Tool for Understanding Invasive Species Management in South Florida
Authors: Harvey Rebecca G. , Mazzotti Frank J.
Published: October 2022

South Florida has more than its share of invasive plants, reptiles, amphibians, fish, mammals, birds, and invertebrates. Nonnative species often spread quickly. Research and monitoring can help managers anticipate which species will cause harm before it is too late to remove them. Identifying where a species is on the invasion curve is the first step to taking management action. The invasion curve shows that eradication of an invasive species becomes less likely and control costs increase as an invasive species spreads over time. Prevention is the most cost-effective solution, followed by eradication. If a species is not detected and removed early, intense and long-term control efforts will be unavoidable. This 4-page fact sheet was written by Rebecca G. Harvey and Frank J. Mazzotti, and published by the UF Department of Wildlife Ecology and Conservation, November 2014. 
WEC347/UW392: The Invasion Curve: A Tool for Understanding Invasive Species Management in South Florida (ufl.edu)
Building Impactful Extension Programs By Understanding How People Change
Authors: Warner Laura A. , Galindo-Gonzalez Sebastian , Gutter Michael S.
Published: October 2022

Extension is reported to be one of the world’s most successful change agencies, and the ability to encourage behavior change remains critical to its success. This 7-page fact sheet describes an approach to understanding how Extension audiences move through the process of change as a means of delivering meaningful programming at the most appropriate level. Written by Laura A. Warner, Sebastian Galindo-Gonzalez, and Michael S. Gutter, and published by the UF Department of Agricultural Education and Communication, December 2014. (Photo: iStock/Thinkstock.com)

AEC527/WC189: Building Impactful Extension Programs By Understanding How People Change (ufl.edu)
Алгоритми авторизації та персоніфікації користувачів освітніх онлайн ресурсів
Authors: Perederii O.S. , Osadchyi V.V.
Published: October 2022

У даній статті розглянуто проблеми аутентифікації користувачів і проблеми безпеки, пов'язані з визначенням особи. Проаналізовано проблеми авторизації за логіном і паролем користувача. Розглянуто можливі вирішення даної проблеми. Розглянуто існуючі алгоритми біометричних систем авторизації. Обґрунтовано необхідність використовувати біометричні параметри для аутентифікації користувачів.
Розробка електронного журналу куратора академічної групи студентів з використанням мови програмування С#
Authors: Chyrkova O.H.
Published: October 2022

Функціонування будь-якої системи управління пов’язано з обігом в ній інформації. Процес керування академічною групою студентів вузу, це перш за все, інформаційний процес, що передбачає виконання таких функцій, як:збір; передачу, обробку, аналіз інформації та прийняття відповідних рішень куратором групи. Забезпечення якісного інформаційного доступу куратора та інших педагогів шляхом видачі вірогідної, своєчасної та достатньої для прийняття управлінських рішень інформації у формі, яка є зручною для використання, є основним призначенням електронного журналу куратора академічної групи.
Аналіз програмних засобів для інформаційної підтримки тренувань з бодібілдингу
Authors: Sharov S.V. , Khromyshev O.V.
Published: October 2022

У статті наведено узагальнені дані з напрямків використання інформаційно-комунікаційних технологій у практиці спорту, зокрема у бодібілдингу. Розглянуті можливості наявних програмних засобів для інформаційного забезпечення тренувань з бодібілдингу.
PENGARUH KOMPENSASI, MOTIVASI, DAN LINGKUNGAN KERJA TERHADAP KINERJA KARYAWAN SWALAYAN LUWES NUSUKAN SURAKARTA
Authors: Lifia Novi , Suddin Alwi , Suddin Alwi , Susanti Retno , Susanti Retno
Published: October 2022

A company always have a lot ways to solve their problem for their employes especially at Luwes Nusukan Supermarket Surakarta the goals are for good impact and for the neatly of the company for the future to reach vision and mision. there are some factors that can influence the employe's performance: compensation, motivation and work environment. the purpose of this research are for: (1) analysis the significant compensation influence to the employes performance, (2) analysis the significant motivation influence to the employes performance, (3) to analysis the significant work environment influence to the employe performance. This research is done with survey method at Luwes Nusukan Supermarket Surakarta. this research is using qulitatif and quantitatif data, the source is using premier and sekunder data. the population of this research are the employes of Luwes Nusukan Supermarket Surakarta that is contain with 70 employes. the sample of the research are 70 employes with using sensus method. the data collection is using quisioner. technique analysis data is using classic asumtion, multiple linear aggresion analysis, desctiptive analysis, t test, f test, and coeficient of determation test. The result is showing that the compensation is have a good impact and not significant with the employes performance, the motivation is have a good impact and significant with the employes performance, the environment also have a good impact and significant to the employes performance.
Development of Educational Tool Game (APE) Scooter Board Sensory for Children With Special Needs
Authors: Selvi Atesya Kesumawati  , M. Haris Satria  , Desi Arisandy
Published: October 2022

This study aims to develop an educational game tool (APE) that can stimulate the sensor-motor abilities of children with special needs who have problems in motor and movement development. APE was developed in addition to playing but also aims to provide education and other experiences according to their developmental age and needs. The research method used is the ADDIE development research method (Analysis, Design, Development, Implementation and Evaluation). The subjects in this study were 20 person children with mild ID and autism, aged 8-12 years consisting of 12 girls and 8 boys. This study involved 4 experts, consisting of 2 learning media experts, 1 psychologist, and 1 Adaptive Physical Educator. Based on the analysis by experts using CVI and CVR, the sensory scooter board game tool obtained a mean score of 0.6 which means it has high validity, in other words the sensory scooter board APE developed is safe, inexpensive (low cost), and stimulates the motor skills of children with special needs.
Pengaruh Penyajian Laporan Keuangan, Aksesibilitas, dan Pengawasan Kualitas Laporan Keuangan terhadap Akuntabilitas Pengelolaan Keuangan Daerah
Authors: Paramayana Asep Nanda , Dewi Riana Rachmawati , Astungkara Agni
Published: October 2022

Government financial statements are intended to provide information useful for decision-making and represent the reporting entity's responsibility for entrusted resources. While financial reporting is required, access to and monitoring of financial reporting quality to enhance accountability in regional financial management. The purpose of this study was to determine the effect of the presentation of financial statements, accessibility, and supervision on the accountability of regional financial management of the Surakarta City DPRD. This research is quantitative. The population consists of 44 employee of the Surakarta City DPRD. The number of samples is 44 employee of the Surakarta City DPRD. The data analysis technique used multiple linear regression analysis. This research is indicated by the results of the presentation of financial statements that affect the accountability of regional financial management to the Surakarta City DPRD. Accessibility affects the accountability of regional financial management to the Surakarta City DPRD. Supervision of the quality of financial reports affects the accountability of regional financial management to the Surakarta City DPRD. The sample is only 44 employee of the Surakarta City DPRD. If the number of samples is increased, better results are likely to be obtained. The next researcher, can add the number of samples of other research objects.
Studi Empiris Kualitas Audit Pada Badan Pemeriksa Keuangan Republik Indonesia Perwakilan Lampung
Authors: Siregar Muhammad Ichsan , Hidayat Muhammad , Saggaf Abdullah , Maryati Sri , Kesuma Nilam
Published: October 2022

This study aims to obtain empirical evidence about the effect of competence, independence and objectivity on audit quality. The population in this study were all auditors who worked at the Supreme Audit Agency of the Republic of Indonesia. Researchers took a sample of 60 auditors who is still active. The data used in this study is primary data. The primary data used by the researcher is in the form of a questionnaire using a series of structured questions relating to the problem to be studied and related to the variables to be studied, namely: Competence, Independence, Objectivity and Audit Quality. Data collection techniques carried out in this study using a questionnaire technique. Researchers obtained data by sending a questionnaire via google form to the auditors of the Lampung Representative Audit Board. While the measuring instrument or research instrument used in this research is to use a Likert scale. Processing and analyzing data in this study using the SPSS program which is used to calculate statistical values in the form of descriptive statistical tests, data quality tests, multiple regression tests, classical assumption tests and hypothesis testing. Data analysis using multiple linear regression analysis is used to determine the direction and how much influence the independent variable has on the dependent variable. Based on the results of the study, it was found that competence and objectivity had a significant effect on audit quality, while independence had no significant effect on audit quality.
Peran Financial Technology Berbasis Digital Payment System E-Wallet dalam Meningkatkan Literasi Keuangan
Authors: Maulidah Hikmatul , Krisdiyawati Krisdiyawati , Utami Erni Unggul Sedya
Published: October 2022

This study aims to determine the role of financial technology based on the digital payment system e-wallet in improving financial literacy in MSMEs. Financial technology based on the digital payment system e-wallet is a financial service that can help MSME actors to make payment transactions easily and automatically provide financial recording facilities related to cash receipt transactions that occur in the business of MSME actors. There are 4 levels of financial literacy, namely not literate, less literate, sufficient literate, and well literate. Financial literacy in this study was measured by knowledge and understanding, risks and benefits, features, security and confidentiality, and skills in using e-wallet used by MSME owners. The study was conducted on 2 MSME coffee shops that use financial technology based on the digital payment system e-wallet, namely Wiji Kopi and Tradition Ngopi. The type of research in this study is qualitative with a case study approach. This research uses spiral data analysis technique. This study uses primary data and secondary data in the form of interviews, observations, literature studies, and documentation. Research results The application of financial technology based on the digital payment system e-wallet facilitates the business activities of MSME coffee shop actors, especially in terms of managing payment transactions in their business so as to increase the financial literacy of coffee shop MSME actors.
Kecerdasan Spiritual sebagai Variabel Moderating pada Pengaruh Narsisme terhadap Perilaku Keputusan Investasi
Authors: Abdillah M. Riduan , Putriana Astia , Anjaswari Gati
Published: October 2022

This research aimed to examine empirically the impact of narcissism on investment decision behavior with spiritual intelligence as a moderating variable. The method used in this research is quantitative. The population of this research is D3 college students of accounting program class of 2020 at Politeknik Negeri Tanah Laut as a State University (PTN) and S1 college students of accounting program class of 2019 and 2020 at Sekolah Tinggi Ilmu Ekonomi Nasional Banjarmasin as Private Universities (PTS). The samples of this research are 195 college students, obtained using the purposive sampling technique. This type of data uses primary data through distributing questionnaires to respondents while path analysis is used to test research hypotheses in order to formulate research results by means of statistical testing through SPSS software and WarpPLS. The result shows that narcissism affects investment decision behavior. This research also finds that spiritual intelligence can not moderate the effect of narcissism on investment decision behavior. These findings help to obtain insight regarding the special concern on approach that can minimize aggressive investment decision behavior which is narcissism. These empirical findings can also be used as benchmarks for similar research, especially in responding to the challenges of the times and the rise of socio-economic phenomena among millennials during the Covid-19 period for universities.
Peran Manajemen Risiko Dalam Memoderasi Rasio Keuangan Terhadap Nilai Perusahaan
Authors: Maychael Maychael , Pangestuti Dewi Cahyani
Published: October 2022

Penelitian ini merupakan penelitian kuantitatif yang ditujukan untuk mengetahui peranan manajemen risiko dalam memoderasi rasio keuangan terhadap nilai perusahaan, serta untuk mengetahui peranan rasio profitabilitas (return on equity ratio), peranan rasio solvabilitas (debt to equity ratio), dan peranan rasio likuiditas (current ratio) terhadap nilai perusahaan (Tobin’s Q). Populasi dalam penelitian ini adalah perusahaan sektor properti di Bursa Efek Indonesia (BEI) periode 2017-2020. Pemilihan sampel yang digunakan adalah purposive sampling, sehingga diperoleh 30 perusahaan dengan periode penelitian 4 tahun, serta memperoleh 120 data penelitian. Analisis data dalam penelitian menggunakan analisis regresi data panel dengan aplikasi E-Views 12 dan tingkat signifikansi sebesar 5%. Hasil dari penelitian secara parsial menunjukkan bahwa : profitabilitas berpengaruh terhadap nilai perusahaan, manajemen risiko tidak memoderasi profitabilitas terhadap nilai perusahaan, solvabilitas berpengaruh terhadap nilai perusahaan, manajemen risiko tidak memoderasi solvabilitas terhadap nilai perusahaan, likuiditas berpengaruh terhadap nilai perusahaan, dan manajemen risiko tidak memoderasi likuiditas terhadap nilai perusahaan.
Pengaruh pengetahuan investasi, manfaat motivasi, dan modal minimal investasi terhadap keputusan investasi di Pasar Modal
Authors: Fitriasuri Fitriasuri , Simanjuntak Rahayu Maharani Abhelia
Published: October 2022

This study aims to determine the effect of investment knowledge, investment benefits, investment motivation and minimal capital on investment decisions by students. The population in this study is data from students majoring in accounting for the 2018 class from several universities in the city of Palembang with the help of the slovin method. While the sample data used were 85 respondents who came from students majoring in accounting. The data collection method used in this study is a qualitative method in which researchers distribute questionnaires that will be filled out by respondents using SPSS version 25 test equipment, Investment Benefits and Investment Motivation have no effect on Investment Decisions. While the Minimum Investment Capital affects investment decisions
Pengaruh Lingkungan Yang Dimoderasi Oleh Ukuran Perusahaan Terhadap Nilai Perusahaan Pada Pandemi Covid-19
Authors: Wibawa Dimas Satria , Khomsiyah Khomsiyah
Published: October 2022

The objective of this study was to investigate the relationship between environmental performance and firm value as well as the relationship between environmental disclosure and firm value. The effect of each of these independent variables on the value of the company as well as the study's objective could also be moderated by the company size. There were 62 samples of companies that were listed on the Indonesia Stock Exchange between 2019 and 2021. Data analysis was examined by using multiple linear regression analysis with Moderate Regression Analysis method using SPSS 25 for further details. As a result, disclosure and environmental performance on firm value were significantly positive. Next, firm size as a moderator of firm value strengthens its effect, which was significant positive. Meanwhile, the effect of environmental performance did not moderate firm value.
Pengaruh Financial Distress, Profitabilitas, dan Leverage terhadap Tax Avoidance
Authors: Fadhila Nida , Andayani Sari
Published: October 2022

Although taxes are considered important, the realization of tax revenues has not been carried out optimally. This is due to the low compliance of taxpayers in Indonesia and there are taxpayers who do tax avoidance. This study aims to prove empirically the effect of financial distress, profitability, and leverage on tax avoidance. The empirical study used is multinational companies in the manufacturing industry and producers of raw materials listed on the Indonesia Stock Exchange from 2017 to 2021. This research is included in quantitative research, using secondary data in the form of an annual report obtained from the official website of the Indonesia Stock Exchange. (IDX) as well as the company's official website. The population in this study was 129 companies, including 92 multinational manufacturing companies and 37 multinational companies producing raw materials. Sampling using purposive sampling technique, namely the method of determining the sample using certain criteria determined by the researcher. After using the purposive sampling method, the sample size was 50 companies with a research period of 5 years, in order to obtain 250 observational data. The method used is multiple linear regression method. The analysis technique is descriptive statistical analysis, classical assumption test and hypothesis testing consisting of goodness of fit (F test), statistical hypothesis test (t test), and coefficient of determination (R2). Data processing was carried out with the help of SPSS version 25 software. The results showed that financial distress and leverage had a positive effect on tax avoidance, while profitability had a negative effect on tax avoidance.
Maximizing Correction of Infertility with Moderate to Marked Diminished Egg Reserve in Natural Cycles by Up-Regulating Follicle Stimulating Hormone Receptors
Authors: Check Jerome H. , Choe Jung K.
Published: October 2022

Many infertility specialists advise women with diminished oocyte reserve (DOR) that their remaining oocytes probably are of poor quality similar to women of advanced reproductive age. There have been studies, especially employing in vitro fertilization-embryo transfer (IVF-ET), showing very poor live delivered pregnancy rates despite the transfer of morphologically normal embryos in women even with mild DOR. However, other data suggests that the low pregnancy rates are related to the use of high dosages of follicle stimulating hormone (FSH) drugs which down-regulate some key FSH dependent enzymes, cytokines, or proteins required for proper embryo implantation. Some studies have shown that techniques that favor FSH receptor up-regulation, rather than down-regulation, can provide the chance of live delivery 80% as well in women ≤ 35 with DOR, 70% for women 36-39, and 50% for women 40-42. Though some infertility specialists will encourage women whose only infertility issue is DOR, who reject the initial suggestion to consider donor oocytes, to proceed immediately with IVF-ET to maximize success, pregnancies are quite possible with natural conception. Thus, it seems imprudent to make couples undergo the financial burden of IVF-ET in the absence of a significant tubal or male factor problem. Not only have live deliveries occurred in women with DOR, using the principle described to achieve a mature dominant follicle followed by proper luteal phase support, with serum FSH levels over 100 mIU/mL, but also serum Anti-Mullerian Hormone (AMH) levels that were undetectable. This even applies to women in overt menopause where FSH up-regulation was achieved by negative feedback to the pituitary using ethinyl estradiol inhibiting FSH release, or down-regulation of hypothalamic-pituitary stimulation of FSH production by using gonadotropin releasing hormone agonists or antagonists.
Sex-Related Differences in Symptoms Among Patients Presenting with Acute Stroke: A Systematic Review and Meta-analysis
Authors: Spooner Caitlin , Spooner Stephanie , Tierney Stephanie
Published: October 2022

Background: Recognising acute stroke symptoms is crucial in providing timely treatment. However, evidence suggests that females often experience unique symptoms compared with males, resulting in delays to seeking medical attention and treatment. This systematic review and meta-analysis evaluated whether sex is associated with differences in acute stroke symptoms.

Methods: Searches from 1946 to 7th September 2021 were carried out using MEDLINE, Embase, Cumulative Index to Nursing and Allied Health Literature (CINAHL), and the Cochrane Library. Studies reporting acute stroke symptoms in adult females and males were eligible for inclusion. Eleven observational studies met the inclusion criteria. Methodological quality was assessed using the Newcastle–Ottawa scale (NOS). Data were meta-analysed using a random-effects model.

Results: Compared with males, females had higher odds of experiencing headache (odds ratio [OR]: 1.27; 95% confidence interval [CI]: 1.01–1.59); change in level of consciousness (OR: 1.36; 95% CI: 1.13–1.63); fatigue (OR: 1.53; 95% CI: 1.04–2.25); and incontinence (OR: 1.44; 95% CI: 1.29–1.60). In contrast, females were at lower odds of experiencing trouble speaking (OR: 0.79; 95% CI: 0.64–0.96); trouble walking, loss of balance, or co-ordination (OR: 0.55; 95% CI: 0.39–0.76); and dizziness (OR: 0.77; 95% CI: 0.64–0.94) compared with males. No difference was found in confusion, difficulty understanding speech, trouble seeing in one or both eyes, mental status change, and nausea or vomiting.

Discussion: Sex differences do exist in some acute stroke symptoms. At the same time, the overlap in symptoms between sexes was substantial. Healthcare professionals and public health campaigns should continue to promote classic symptoms of acute stroke, whilst taking into account the less common symptoms and the potential differences in symptoms experienced by females and males.
Consumer attitudes towards egg production systems and hen welfare across the world
Authors: Sinclair Michelle , Lee Natasha Y. P. , Hötzel Maria José , de Luna Maria Catalina T. , Sharma Arvind , Idris Musadiq , Islam Mohammad Ariful , Iyasere Oluwaseun S. , Navarro Grisel , Ahmed Abdelkareem A. , Curry Michael , Burns Georgette Leah , Marchant Jeremy N.
Published: October 2022

Concurrent with the growth of the human population, global egg production has experienced exponential increase in the last 50 years. This exponential growth has been made possible through the refinement of intensive egg production systems, predominately, conventional cages. The significant amount of liberties conventional cages withhold from hens has also formed the basis of growing challenge to the industry in many countries, with some major regions such as the European Union making sweeping regulatory reform. These reforms were driven by well-organised animal advocacy groups, fuelled by aligning consumer attitudes and behaviours. In the absence of concerted hen welfare advocacy and cage-free movement in much of the rest of the world, it is difficult to know what consumer attitudes and knowledge may exist outside the European Union. This study aimed to contribute to this knowledge by surveying 4,292 members of the public at random across 14 geographically and culturally diverse countries (Australia, Bangladesh, Brazil, Chile, China, India, Malaysia, Nigeria, Pakistan, Philippines, Sudan, Thailand, UK and USA). The findings show that most participants across all countries eat eggs, most state that it matters to them that hens do not suffer in the process of producing the eggs they eat, and importantly, a majority of participants in most countries (except Nigeria) would prefer (to varying degrees) to purchase eggs from hens not kept in cages. Participant knowledge of the dominant system of egg production in their country varied greatly, with frequent uncertainty. This suggests a need for clarity and consistency in communication to consumers during campaigns, in order to mitigate confusion. Most importantly, although these similarities existed across the countries, important regional differences were also present. This underscores the importance of understanding animal and agricultural issues by geopolitical region, and of locally tailoring strategy. The findings of this research will be of strategic use to egg producers and animal welfare advocates alike in understanding and appropriately catering to consumers in the future.
Recent advances in hydrogels-based osteosarcoma therapy
Authors: Tian Hao , Wu Ronghui , Feng Na , Zhang Jinrui , Zuo Jianlin
Published: October 2022

Osteosarcoma (OS), as a typical kind of bone tumors, has a high incidence among adolescents. Traditional tumor eradication avenues for OS such as chemotherapy, surgical therapy and radiation therapy usually have their own drawbacks including recurrence and metastasis. In addition, another serious issue in the treatment of OS is bone repair because the bone after tumor invasion usually has difficulty in repairing itself. Hydrogels, as a synthetic or natural platform with a porous three-dimensional structure, can be applied as desirable platforms for OS treatment. They can not only be used as carriers for tumor therapeutic drugs but mimic the extracellular matrix for the growth and differentiation of mesenchymal stem cells (MSCs), thus providing tumor treatment and enhancing bone regeneration at the same time. This review focuses the application of hydrogels in OS suppression and bone regeneration, and give some suggests on future development.
A transgenic bacterial artificial chromosome approach to identify regulatory regions that direct Amhr2 and Osterix expression in Müllerian duct mesenchyme
Authors: Moses Malcolm M. , Mullen Rachel D. , Idowu Daniel I. , Maye Peter , Jamin Soazik P. , Behringer Richard R.
Published: October 2022

A transgenic mouse approach using bacterial artificial chromosomes (BAC) was used to identify regulatory regions that direct Müllerian duct expression for Amhr2 and Osterix (Osx, also known as Sp7). Amhr2 encodes the receptor that mediates anti-Müllerian hormone (AMH) signaling for Müllerian duct regression in male embryos. Amhr2 is expressed in the Müllerian duct mesenchyme of both male and female embryos. A ∼147-kb BAC clone containing the Amhr2 locus was used to generate transgenic mice. The transgene was able to rescue the block in Müllerian duct regression of Amhr2-null males, suggesting that the BAC clone contains regulatory sequences active in male embryos. Osx is expressed in the developing skeleton of male and female embryos but is also an AMH-induced gene that is expressed in the Müllerian duct mesenchyme exclusively in male embryos. Osx-Cre transgenic mice were previously generated using a ∼204-kb BAC clone. Crosses of Osx-Cre mice to Cre-dependent lacZ reporter mice resulted in reporter expression in the developing skeleton and in the Müllerian duct mesenchyme of male but not female embryos. Osx-Cherry transgenic mice were previously generated using a 39-kb genomic region surrounding the Osx locus. Osx-Cherry embryos expressed red fluorescence in the developing skeleton and Müllerian duct mesenchyme of male but not female embryos. In addition, female Osx-Cherry embryos ectopically expressing human AMH from an Mt1-AMH transgene activated red fluorescence in the Müllerian duct mesenchyme. These results suggest that the 39-kb region used to generate Osx-Cherry contains male-specific Müllerian duct mesenchyme regulatory sequences that are responsive to AMH signaling. These BAC transgenic mouse approaches identify two distinct regions that direct Müllerian duct mesenchyme expression and contribute fundamental knowledge to define a gene regulatory network for sex differentiation.
Umbilical cord mesenchymal stromal cells—from bench to bedside
Authors: Chetty Shashank , Yarani Reza , Swaminathan Ganesh , Primavera Rosita , Regmi Shobha , Rai Sravanthi , Zhong Jim , Ganguly Abantika , Thakor Avnesh S
Published: October 2022

In recent years, mesenchymal stromal cells (MSCs) have generated a lot of attention due to their paracrine and immuno-modulatory properties. mesenchymal stromal cells derived from the umbilical cord (UC) are becoming increasingly recognized as having increased therapeutic potential when compared to mesenchymal stromal cells from other sources. The purpose of this review is to provide an overview of the various compartments of umbilical cord tissue from which mesenchymal stromal cells can be isolated, the differences and similarities with respect to their regenerative and immuno-modulatory properties, as well as the single cell transcriptomic profiles of in vitro expanded and freshly isolated umbilical cord-mesenchymal stromal cells. In addition, we discuss the therapeutic potential and biodistribution of umbilical cord-mesenchymal stromal cells following systemic administration while providing an overview of pre-clinical and clinical trials involving umbilical cord-mesenchymal stromal cells and their associated secretome and extracellular vesicles (EVs). The clinical applications of umbilical cord-mesenchymal stromal cells are also discussed, especially in relation to obstacles and potential solutions for their effective translation from bench to bedside.
FOXO1 regulates pentose phosphate pathway-mediated induction of developmental erythropoiesis
Authors: Monsalve Anuntxi , Canals Isaac , Oburoglu Leal
Published: October 2022

Primitive, neonatal and adult erythroid cells have been previously shown to have an active pentose phosphate pathway (PPP) that fuels various processes. However, it is unclear whether the PPP plays a role during the emergence of erythroid progenitors from hemogenic endothelium (HE). In this study, we explored PPP and its genetic regulation in developmental erythropoiesis. We induced hematopoietic differentiation of human induced pluripotent stem cells (hiPSCs) to obtain HE cells. These cells were treated with lentiviral vectors harboring shRNAs against FOXO1, or with inhibitors against the PPP, NRF2 or AKT. Erythroid differentiation, proliferation and frequency were evaluated by flow cytometry. Gene expression was assessed by qPCR or by analysis of available RNAseq data. We found that PPP is indispensable for the erythroid differentiation of HE cells and it partially fuels nucleotide biosynthesis. Moreover, we showed that NRF2 and AKT are essential, while FOXO1 is detrimental, for HE-derived erythroid differentiation. In contrast, blocking FOXO1 expression did not affect erythroid differentiation of cord-blood HSPCs. Mechanistically, FOXO1 inhibition in HE cells led to an increase in the non-oxidative branch of the PPP. During developmental erythropoiesis, the gradual decrease in FOXO1 activates the PPP and fuels nucleotide biosynthesis and cell proliferation.
The prognostic marker FLVCR2 associated with tumor progression and immune infiltration for acute myeloid leukemia
Authors: Su Xiuhua , Ma Guangxin , Bai Xiaoran , Zhang Juan , Li Mingying , Zhang Fan , Sun Tao , Ma Daoxin , Lu Fei , Ji Chunyan
Published: October 2022

Acute myeloid leukemia (AML) is one of the most common hematopoietic malignancies in adults. The tumor microenvironment (TME) has a critical effect on AML occurrence, recurrence, and progression. The gene feline leukemia virus subgroup C cellular receptor family member 2 (FLVCR2) belongs to the major facilitator superfamily of transporter protein members, which is primarily involved in transporting small molecules. The potential role of FLVCR2 in the TME in AML has not been investigated. To clarify the expression and role of FLVCR2 in AML, we analyzed the Gene Expression Omnibus and The Cancer Genome Atlas databases and found that FLVCR2 mRNA expression significantly increased among patients with AML. Furthermore, based on an analysis of the Gene Expression Profiling Interactive Analysis database, FLVCR2 upregulation predicted dismal overall survival of patients with AML. Our validation analysis revealed the significant upregulation of FLVCR2 within the bone marrow of AML relative to healthy controls by western blotting and qPCR assays. Gene set enrichment analysis was conducted to explore FLVCR2’s related mechanism in AML. We found that high FLVCR2 expression was related to infiltration degrees of immune cells and immune scores among AML cases, indicating that FLVCR2 possibly had a crucial effect on AML progression through the immune response. Specifically, FLVCR2 upregulation was negatively related to the immune infiltration degrees of activated natural killer cells, activated memory CD4+ T cells, activated dendritic cells, and CD8+ T cells using CIBERSORT analysis. According to the in vitro research, FLVCR2 silencing suppressed AML cell growth and promoted their apoptosis. This study provides insights into FLVCR2’s effect on tumor immunity, indicating that it might serve as an independent prognostic biomarker and was related to immune infiltration within AML.
Flame-retardant properties and mechanism of LGF/PBT/DOPO-HQ-conjugated flame-retardant composites
Authors: Sun Junzhuo , Zhang Daohai , Shang Xiaoyu , Tan Fang , Bao Dongmei , Qin Shuhao
Published: October 2022

In this article, long fiber reinforced polybutylene terephthalate (LGF/PBT/DOPO-HQ) flame-retardant composites were prepared using 10-(2,5-dihydroxy phenyl)-10H-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO-HQ) as the conjugated flame-retardant. The effects of different flame-retardant contents on the combustion properties of the composites were investigated. The results showed that after adding 14% of DOPO-HQ, the flame-retardant effect of the composite reached the V-0 level of UL-94 fire rating with an ultimate oxygen index (LOI) of 26.4%. The average heat release rate (Av-HRR), peak heat release rate (PHRR), and total heat release rate (THR) decreased by 45.9, 56.5, and 32.6%, respectively. This shows that LGF/PBT/DOPO-HQ composite has good flame-retardant properties. Meanwhile, the flame-retardant mechanism of cohesive phase and gas-phase synergy during the combustion of flame retardants was analyzed by carbon layer morphology and dynamic thermal decomposition.
Triglyceride-glucose index and non-culprit coronary plaque characteristics assessed by optical coherence tomography in patients following acute coronary syndrome: A cross-sectional study
Authors: Zhao Zi-Wei , Liu Chi , Zhao Qi , Xu Ying-Kai , Cheng Yu-Jing , Sun Tie-Nan , Zhou Yu-Jie
Published: October 2022

Background

Triglyceride-glucose (TyG) index, a novel surrogate marker of insulin resistance, has been demonstrated to be significantly associated with cardiovascular disease. It remains indistinct regarding the association between TyG index and non-culprit coronary plaque characteristics in patients following acute coronary syndrome (ACS).

Methods

The present study retrospectively recruited patients who were diagnosed with ACS and underwent non-culprit optical coherence tomography (OCT) examination. The study population was divided into 2 groups based on the median of TyG index, which was calculated as Ln [fasting triglyceride (TG) (mg/dL) × fasting blood glucose (FBG) (mg/dL)/2]. The non-culprit plaque characteristics were determined by interpreting OCT images in accordance with the standard of previous consensus.

Results

110 patients (54.8 ± 12.1 years, 24.5% female) with 284 non-culprit plaques were included in the current analysis. TyG index was closely associated with high-risk plaque characteristics. Elevated TyG index was consistent to be an independent indicator for thin-cap fibroatheroma (TCFA) [odds ratio (OR) for per 1-unit increase 4.940, 95% confidence interval (CI) 1.652–14.767, P = 0.004; OR for taking lower median as reference 2.747, 95% CI 1.234–7.994, P = 0.011] and ruptured plaque (OR for per 1–unit increase 7.065, 95% CI 1.910–26.133, P = 0.003; OR for taking lower median as reference 4.407, 95% CI 1.208–16.047, P = 0.025) in fully adjusted model. The predictive value of TyG index for TCFA and ruptured plaque was moderate–to–high, with the area under the receiver operating characteristic curve (AUC) of 0.754 and 0.699 respectively. The addition of TyG index into a baseline model exhibited an incremental effect on the predictive value for TCFA, manifested as an increased AUC (0.681, 95% CI 0.570–0.793 vs. 0.782, 95% CI 0.688–0.877, P = 0.042), and significant continuous net reclassification improvement (0.346, 95% CI 0.235–0.458, P < 0.001) and integrated discrimination improvement (0.221, 95% CI 0.017–0.425, P = 0.034). TyG index failed to play an incremental effect on predicting ruptured plaque.

Conclusion

TyG index, which is simply calculated from fasting TG and FBG, can be served as an important and independent risk predictor for high-risk non-culprit coronary plaques in patients following ACS.
Comparison of machine learning and logistic regression as predictive models for adverse maternal and neonatal outcomes of preeclampsia: A retrospective study
Authors: Zheng Dongying , Hao Xinyu , Khan Muhanmmad , Wang Lixia , Li Fan , Xiang Ning , Kang Fuli , Hamalainen Timo , Cong Fengyu , Song Kedong , Qiao Chong
Published: October 2022

Introduction

Preeclampsia, one of the leading causes of maternal and fetal morbidity and mortality, demands accurate predictive models for the lack of effective treatment. Predictive models based on machine learning algorithms demonstrate promising potential, while there is a controversial discussion about whether machine learning methods should be recommended preferably, compared to traditional statistical models.

Methods

We employed both logistic regression and six machine learning methods as binary predictive models for a dataset containing 733 women diagnosed with preeclampsia. Participants were grouped by four different pregnancy outcomes. After the imputation of missing values, statistical description and comparison were conducted preliminarily to explore the characteristics of documented 73 variables. Sequentially, correlation analysis and feature selection were performed as preprocessing steps to filter contributing variables for developing models. The models were evaluated by multiple criteria.

Results

We first figured out that the influential variables screened by preprocessing steps did not overlap with those determined by statistical differences. Secondly, the most accurate imputation method is K-Nearest Neighbor, and the imputation process did not affect the performance of the developed models much. Finally, the performance of models was investigated. The random forest classifier, multi-layer perceptron, and support vector machine demonstrated better discriminative power for prediction evaluated by the area under the receiver operating characteristic curve, while the decision tree classifier, random forest, and logistic regression yielded better calibration ability verified, as by the calibration curve.

Conclusion

Machine learning algorithms can accomplish prediction modeling and demonstrate superior discrimination, while Logistic Regression can be calibrated well. Statistical analysis and machine learning are two scientific domains sharing similar themes. The predictive abilities of such developed models vary according to the characteristics of datasets, which still need larger sample sizes and more influential predictors to accumulate evidence.
Risk factors for left atrial thrombus in younger patients (aged < 65 years) with atrial fibrillation or atrial flutter: Data from the multicenter left atrial thrombus on transesophageal echocardiography (LATTEE) registry
Authors: Uziȩbło-Życzkowska Beata , Kapłon-Cieślicka Agnieszka , Gawałko Monika , Budnik Monika , Starzyk Katarzyna , Wożakowska-Kapłon Beata , Daniłowicz-Szymanowicz Ludmiła , Kaufmann Damian , Wójcik Maciej , Błaszczyk Robert , Hiczkiewicz Jarosław , Łojewska Katarzyna , Mizia-Stec Katarzyna , Wybraniec Maciej , Kosmalska Katarzyna , Fijałkowski Marcin , Szymańska Anna , Gos Aleksandra , Haberka Maciej , Kucio Michał , Michalski Błazej , Kupczyńska Karolina , Tomaszuk-Kazberuk Anna , Wilk-Śledziewska Katarzyna , Wachnicka-Truty Renata , Koziński Marek , Burchardt Paweł , Krzesiński Paweł
Published: October 2022

Background

Our aim was to assess the characteristics and to identify predictors of left atrial thrombus (LAT) in patients under age 65 with atrial fibrillation (AF) or atrial flutter (AFl).

Methods

We conducted a subanalysis of a multicenter, prospective, observational study [the LATTEE registry]. Consecutive AF/AFl patients referred for cardioversion or ablation were enrolled.

Results

Of the 3,109 patients included in the study, 1,276 were under age 65 (41%). Compared to non-LAT patients, those with LAT (n = 76) had higher CHA2DS2-VASc score (p < 0.001), more frequently had non-paroxysmal AF/AFl (p < 0.001), heart failure (p < 0.001), history of diabetes mellitus (p = 0.001), transient ischemic attack (p = 0.04), coronary artery disease (p = 0.02), and chronic kidney disease (p < 0.001). The LAT patients were also more often smokers (p = 0.004) and were more frequently treated with vitamin K antagonists (VKAs) (p < 0.001). Transthoracic echocardiography revealed a higher left atrial area (p < 0.001), lower left ventricular ejection fraction (LVEF) (p < 0.001), and lower value of LA appendage emptying volume in LAT than in non-LAT patients (p < 0.001). LVEF (OR 2.95; 95% CI: 1.32–6.59, p = 0.008), non-paroxysmal AF/AFl (OR 7.1; 95% CI: 2.05–24.63, p = 0.002) and treatment with VKAs (OR 4.92; 95% CI: 2.48–9.75, p < 0.001) were identified as independent predictors of LAT in younger patients.

Conclusions

Our study, which focused on younger patients with AF/AFl, indicated substantial clinical and echocardiographic differences between participants with and without LAT. In the AF/AFl patients younger than age 65, the independent predictors of LAT included non-paroxysmal AF/AFl, lower LVEF, and treatment with VKAs.
Right ventricular function in transcatheter mitral and tricuspid valve edge-to-edge repair
Authors: Stolz Lukas , Doldi Philipp M. , Weckbach Ludwig T. , Stocker Thomas J. , Braun Daniel , Orban Martin , Wild Mirjam G. , Hagl Christian , Massberg Steffen , Näbauer Michael , Hausleiter Jörg , Orban Mathias
Published: October 2022

Since transcatheter edge-to-edge repair (TEER) has become a valuable therapy in the treatment of both, mitral (MR) and tricuspid regurgitation (TR), the question of optimized patient selection has gained growing importance. After years of attributing rather little attention to the right ventricle (RV) and its function in the setting of valvular heart failure, this neglect has recently changed. The present review sought to summarize anatomy and function of the RV in a clinical context and aimed at presenting the current knowledge on how the RV influences outcomes after TEER for atrioventricular regurgitation. The anatomy of the RV is determined by its unique shape, which necessitates to use three-dimensional imaging methods for detailed and comprehensive characterization. Complex parameters such as RV to pulmonary artery coupling (RVPAc) have been developed to combine information of RV function and afterload which is primary determined by the pulmonary vasculature and LV filling pressure. Beyond that, TR, which is closely related to RV function also plays an important role in the setting of TEER. While mitral valve transcatheter edge-to-edge repair (M-TEER) leads to reduction of concomitant TR in some patients, the prognostic value of TR in the setting of M-TEER remains unclear. Overall, this review summarizes the current state of knowledge of the outstanding role of RV function and associated TR in the setting of TEER and outlines the unsolved questions associated with right-sided heart failure.