bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Plasma-Activated Water Treatment on the Inactivation of Microorganisms Present on Cherry Tomatoes and in Used Wash Solution
Authors: Lee Gaeul , Choi Sung-Wook , Yoo Miyoung , Chang Hyun-Joo , Lee Nari
Published: June 2023

Herein, we investigated the potential of plasma-activated water (PAW) as a wash solution for the microbial decontamination of cherry tomatoes. We analyzed the efficacy of PAW as a bactericidal agent based on reactive species and pH. Immersion for 5 min in PAW15 (generated via plasma activation for 15 min) was determined as optimal for microbial decontamination of fresh produce. The decontamination efficacy of PAW15 exceeded those of mimic solutions with equivalent reactive species concentrations and pH (3.0 vs. 1.7 log reduction), suggesting that the entire range of plasma-derived reactive species participates in decontamination rather than a few reactive species. PAW15-washing treatment achieved reductions of 6.89 ± 0.36, 7.49 ± 0.40, and 5.60 ± 0.05 log10 CFU/g in the counts of Bacillus cereus, Salmonella sp., and Escherichia coli O157:H7, respectively, inoculated on the surface of cherry tomatoes, with none of these strains detected in the wash solution. During 6 days of 25 °C storage post-washing, the counts of aerobic bacteria, yeasts, and molds were below the detection limit. However, PAW15 did not significantly affect the viability of RAW264.7 cells. These results demonstrate that PAW effectively inactivates microbes and foodborne pathogens on the surface of cherry tomatoes and in the wash solution. Thus, PAW could be used as an alternative wash solution in the fresh produce industry without cross-contamination during washing and environmental contamination by foodborne pathogens or potential risks to human health.
Isolation and Characterization of Yeast with Benzenemethanethiol Synthesis Ability Isolated from Baijiu Daqu
Authors: Zhang Guihu , Xiao Peng , Xu Youqiang , Li Honghua , Li Hehe , Sun Jinyuan , Sun Baoguo
Published: June 2023

Baijiu, a prevalent alcoholic beverage, boasts over 2000 aroma compounds, with sulfur-containing compounds being the most influential in shaping its flavor. Benzenemethanethiol, a distinctive odorant in baijiu, is known to enhance the holistic flavor profile of baijiu. Despite its importance, there is very little literature on the biotransformation mechanism of benzenemethanethiol. Thus, extensive research efforts have been made to elucidate the formation mechanism of this compound in order to improve baijiu production. In this study, 12 yeast strains capable of generating benzenemethanethiol were isolated from baijiu daqu, and the Saccharomyces cerevisiae strain J14 was selected for further investigation. The fermentation conditions were optimized, and it was found that the optimal conditions for producing benzenemethanethiol were at 28 °C for 24 h with a 4% (v/v) inoculum of 3.025 g/L L-cysteine. This is the first time that yeast has been shown to produce benzenemethanethiol isolated from the baijiu fermentation system. These findings also suggest that benzenemethanethiol can be metabolized by yeast using L-cysteine and benzaldehyde as precursor substrates.
Effect of Biopolymer Dip-Coating Pretreatments as a Non-Thermal Green Technology on Physicochemical Characteristics, Drying, and Rehydration Kinetics of Santa Maria Pears
Authors: Kian-Pour Nasim
Published: June 2023

This research was conducted to determine the influences of biopolymer dip-coating pretreatments as a non-thermal green technology on the drying behavior, retention of bioactive compounds, and quality properties of pears. The fresh pears were washed, peeled, and diced into cubes of 5 × 5 mm with a 2 mm thickness and were dipped into 0.3% (w/v) solutions of sodium alginate (SA), pectin (PC), xanthan gum (XG), Arabic gum (AG), and gelatin (GE) before hot air drying (70 °C, 2.0 m/s). The weight loss of samples during drying was recorded online, and the moisture ratio (MR) and drying rate were plotted against drying time. Biopolymers significantly decreased the drying time (maximum 33.33% by SA) compared with uncoated samples except for XG. Moisture diffusion coefficients were determined according to Fick’s second law of diffusion by plotting LnMR against drying time, and a linear regression analysis was applied to the data for the determination of moisture diffusion coefficients which ranged from 2.332 to 3.256 × 10−9 m2/s. The molecular transport of momentum, heat, and mass were determined from Newton’s law of viscosity, Fourier’s law, and Fick’s law, respectively. The results indicated that the friction drag force, convective heat, and mass transfer coefficients were 6.104 × 10−6 N, 76.55 W/m2·K, and 0.0636 m/s, respectively. Mathematical modeling showed the suitability of the Midilli and Kucuk and the Peleg models for the prediction of drying and rehydration processes, respectively. Thermal conductivity, specific heat, and density of coated samples ranged from 0.559–0.579 (W/m·K), 3735–3859 (J/kg·K), and 850.90–883.26 (Kg/m3), respectively. The porosity was reduced due to the penetration of biopolymers into the cellular matrix of samples. The highest total polyphenol content and antioxidant activity belonged to the AG samples. The biopolymers covering the surface of samples produced a protection layer against the loss of bioactive compounds. Biopolymers can be successfully used as a non-thermal green process for improving the drying and quality characteristics of pears at the industrial level.
Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum)
Authors: Lučić Milica , Potkonjak Nebojša , Sredović Ignjatović Ivana , Lević Steva , Dajić-Stevanović Zora , Kolašinac Stefan , Belović Miona , Torbica Aleksandra , Zlatanović Ivan , Pavlović Vladimir , Onjia Antonije
Published: June 2023

This study investigates the effects of ultrasound, in combination with chemical pretreatments, on the quality attributes (total phenolic and carotenoid content, antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl assay (DPPH)), ferric-reducing ability (FRAP), CIE L* a* b* color, non-enzymatic browning, rehydration ratio, textural and morphological properties) of red pepper subjected to drying (hot air drying or freeze drying). The fractional factorial design was used to assess the impact of factors. The global Derringer desirability function was used to determine the optimal conditions for the best quality attributes of dried pepper. The drying method influenced total phenolic content, a* (redness), and initial rehydration ratio; pretreatment time significantly affected FRAP antiradical activity, a*, chroma and non-browning index, while pH-value had a significant effect on the texture of dried pepper. Non-enzymatic browning was reduced to 72.6%, while the DPPH antioxidant capacity of freeze-dried peppers was enhanced from 4.2% to 71.9%. Ultrasonic pretreatment led to changes in the pepper morphology, while potassium metabisulfite (KMS) was a more effective additive than citric acid.
Texture Feature Extraction from 1H NMR Spectra for the Geographical Origin Traceability of Chinese Yam
Authors: Hu Zhongyi , Luo Zhenzhen , Wang Yanli , Zhou Qiuju , Liu Shuangyan , Wang Qiang
Published: June 2023

Adulteration is widespread in the herbal and food industry and seriously restricts traditional Chinese medicine development. Accurate identification of geo-authentic herbs ensures drug safety and effectiveness. In this study, 1H NMR combined intelligent “rotation-invariant uniform local binary pattern” identification was implemented for the geographical origin confirmation of geo-authentic Chinese yam (grown in Jiaozuo, Henan province) from Chinese yams grown in other locations. Our results showed that the texture feature of 1H NMR image extracted with rotation-invariant uniform local binary pattern for identification is far superior compared to the original NMR data. Furthermore, data preprocessing is necessary. Moreover, the model combining a feature extraction algorithm and support vector machine (SVM) classifier demonstrated good robustness. This approach is advantageous, as it is accurate, rapid, simple, and inexpensive. It is also suitable for the geographical origin traceability of other geographical indication agricultural products.
Optimization of Exopolysaccharide Produced by Lactobacillus plantarum R301 and Its Antioxidant and Anti-Inflammatory Activities
Authors: Wang Junyong , Zhang Jing , Guo Henan , Cheng Qiang , Abbas Zaheer , Tong Yucui , Yang Tiantian , Zhou Yichen , Zhang Haosen , Wei Xubiao , Si Dayong , Zhang Rijun
Published: June 2023

In this study, the yield of exopolysaccharide (EPS) from Lactobacillus plantarum R301 was optimized using a single-factor experiment and response surface methodology (RSM). After optimization, the EPS yield was increased with a fold-change of 0.85. The significant factors affecting EPS production, as determined through a Plackett–Burman design and Central Composite Design (CCD), were MgSO4 concentration, initial pH, and inoculation size. The maximum yield was 97.85 mg/mL under the condition of 0.01% MgSO4, an initial pH 7.4, and 6.4% of the inoculation size. In addition, the EPS exhibited strong antioxidant activity, as demonstrated by its ability to scavenge DPPH, ABTS, and hydroxyl radicals. The scavenging rate was up to 100% at concentrations of 4 mg/mL, 1 mg/mL, and 2 mg/mL, respectively. Moreover, the EPS also exhibited reducing power, which was about 30% that of ascorbic acid when both tended to be stable with the increased concentration. These results suggest that L. plantarum R301 EPS possesses different antioxidant mechanisms and warrants further investigation. In addition to its antioxidant activity, the EPS also demonstrated good anti-inflammatory activity by inhibiting the inflammation induced by lipopolysaccharide (LPS) in RAW 264.7 cells, which could decrease nitric oxide (NO) production and expression of the proinflammatory cytokine Il-6. These findings suggest that L. plantarum R301 EPS could be used as a potential multifunctional food additive in the food industry.
Influence of Proteins on Bioaccessibility of α-Tocopherol Encapsulation within High Diacylglycerol-Based Emulsions
Authors: Zou Qian , Wang Weifei , Xu Qingqing , Yan Menglei , Lan Dongming , Wang Yonghua
Published: June 2023

α-Tocopherol has been widely used in medicine, cosmetics, and food industry as a nutritional supplement and antioxidant. However, α-tocopherol showed low bioaccessibility, and there is a widespread α-tocopherol deficiency in society today. The preparation of oil-in-water emulsions with high safety and low-calorie property is necessary. The aim of this research was to investigate the effects of different protein emulsifiers (whey protein isolate (WPI), soy protein isolate (SPI), and sodium casein (SC)) on the properties of emulsions delivery system, and diacylglycerol (DAG) was picked as a low-accumulated lipid. The interfacial changes, microstructural alterations, and possible interactions of the protein-stabilized DAG emulsions were investigated during the in vitro digestion. The results show that different proteins affect the degree of digestibility and α-tocopherol bioaccessibility of the emulsions. Both WPI- and SPI-coated emulsions showed good digestibility and α-tocopherol bioaccessibility (77.64 ± 2.93%). This might be due to the strong hydrolysis resistance of WPI (β-lactoglobulin) and the good emulsification ability of SPI. The SC-coated emulsion showed the lowest digestibility and α-tocopherol bioaccessibility, this might be due to the emulsification property of hydrolysis products of SC and the potential interaction with calcium ions. This study provides new possibilities for the application of DAG emulsions in delivery systems.
Detection of Specific Volatile Organic Compounds in Tribolium castaneum (Herbst) by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry
Authors: Han Shaoyun , He Ke , An Jing , Qiao Mengmeng , Ke Runhui , Wang Xiao , Xu Yang , Tang Xiuying
Published: June 2023

The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is a major storage pest that could lead to a wide range of damage. Its secretions have a significant impact on the quality of stored grain and food, leading to serious food safety problems such as grain spoilage and food carcinogenesis. This study investigates new detection techniques for grain storage pests to improve grain insect detection in China. The primary volatile organic chemicals (VOCs) in these secretions are identified using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The specific VOCs that are unique to T. castaneum are selected as criteria for determining the presence of T. castaneum in the granary. To obtain more specific VOCs, experiments were designed for the analysis of T. castaneum samples under different extraction times, two types of SPME fibers and two GC-MS devices of different manufacturers. The experimental results showed that 12 VOCs were detected at relatively high levels, seven of which were common and which were not detected in other grains and grain insects. The seven compounds are 1-pentadecene, 2-methyl-p-benzoquinone, 2-ethyl-p-benzoquinone, 1-hexadecene, cis-9-tetradecen-1-ol, m-cresol and paeonol. These seven compounds can be used as volatile markers to identify the presence of T. castaneum, which could serve as a research foundation for the creation of new techniques for T. castaneum monitoring.
A Turn-On Fluorescence Sensor Based on Nitrogen-Doped Carbon Dots and Cu2+ for Sensitively and Selectively Sensing Glyphosate
Authors: Li Ziqiang , Liang Shuang , Zhou Li , Luo Fengjian , Lou Zhengyun , Chen Zongmao , Zhang Xinzhong , Yang Mei
Published: June 2023

Glyphosate has excellent herbicidal activity, and its extensive use may induce residue in the environment and enter into humans living through the food chain, causing negative impact. Here, water-soluble 1.55 nm size nitrogen-doped carbon quantum dots (NCDs) with strong blue fluorescence were synthesized using sodium citrate and adenine. The maximum excitation and emission wavelengths of NCDs were 380 nm and 440 nm, respectively. The above synthesized NCDs were first used for the construction of a fluorescence sensor for glyphosate detection. It was found that Cu2+ could quench the fluorescence of NCDs effectively through the photoinduced electron transfer (PET) process, which was confirmed using fluorescence lifetime measurements. Additionally, the fluorescence was restored with the addition of glyphosate. Hence, a sensitive turn-on fluorescence sensor based on NCDs/Cu2+ for glyphosate analysis was developed. The LODs of glyphosate for water and rice samples were recorded as 0.021 μg/mL and 0.049 μg/mL, respectively. The sensor was applied successfully for ultrasensitive and selective detection of glyphosate in environmental water and rice samples with satisfied recoveries from 82.1% to 113.0% using a simple sample pretreatment technique. The proposed strategy can provide a significant potential for monitoring glyphosate residue in water and agricultural product samples.
Selection of Reliable Reference Genes for Gene Expression Normalization in Sagittaria trifolia
Authors: Tang Jing , Li Enjiao , Liu Jiexia , Zhang Zhiping , Hua Bing , Jiang Jiezeng , Miao Minmin
Published: June 2023

Real-time quantitative PCR (RT-qPCR) is a method with high sensitivity and convenience that has been extensively used to analyze the expression level of target genes. A reference gene with a highly stable expression is required to ensure the accuracy of experimental results. However, the report on appropriate reference genes in arrowheads (Sagittaria trifolia) is still limited. In this study, eight candidate reference genes (ACT5, UBQ, GAPDH, CYP, NAC, IDH, SLEEPER and PLA) were selected. The candidate genes were employed in a RT-qPCR assay in different tissues at different developmental stages of the same tissue (including corm, leaf and leafstalk) in arrowheads. Five statistical algorithms, GeNorm, NormFinder, BestKeeper, delta cycle threshold (ΔCt) and RefFinder, were used to evaluate the stability of these genes’ expressions in order to identify the appropriate reference genes. The results showed that UBQ was the optimum reference gene in leaf, leafstalk, root, stolon and corm, IDH exhibited the most stable expression during the expansion of corm, UBQ and PLA were the most stable reference genes in developmental stages of leaf and leafstalk, respectively. Finally, the reliability of reference genes was further confirmed by the normalization of PDS and EXP1 genes under different arrowhead tissues and developmental stages of corm, respectively. This study constitutes important guidance for the selection of reliable reference genes for analyzing the tissue- and developmental-stage-specific expression of genes in arrowheads.
Genome-Wide Epistasis Study of Cerebrospinal Fluid Hyperphosphorylated Tau in ADNI Cohort
Authors: Chen Dandan , Li Jin , Liu Hongwei , Liu Xiaolong , Zhang Chenghao , Luo Haoran , Wei Yiming , Xi Yang , Liang Hong , Zhang Qiushi
Published: June 2023

Alzheimer’s disease (AD) is the main cause of dementia worldwide, and the genetic mechanism of which is not yet fully understood. Much evidence has accumulated over the past decade to suggest that after the first large-scale genome-wide association studies (GWAS) were conducted, the problem of “missing heritability” in AD is still a great challenge. Epistasis has been considered as one of the main causes of “missing heritability” in AD, which has been largely ignored in human genetics. The focus of current genome-wide epistasis studies is usually on single nucleotide polymorphisms (SNPs) that have significant individual effects, and the amount of heritability explained by which was very low. Moreover, AD is characterized by progressive cognitive decline and neuronal damage, and some studies have suggested that hyperphosphorylated tau (P-tau) mediates neuronal death by inducing necroptosis and inflammation in AD. Therefore, this study focused on identifying epistasis between two-marker interactions at marginal main effects across the whole genome using cerebrospinal fluid (CSF) P-tau as quantitative trait (QT). We sought to detect interactions between SNPs in a multi-GPU based linear regression method by using age, gender, and clinical diagnostic status (cds) as covariates. We then used the STRING online tool to perform the PPI network and identify two-marker epistasis at the level of gene–gene interaction. A total of 758 SNP pairs were found to be statistically significant. Particularly, between the marginal main effect SNP pairs, highly significant SNP–SNP interactions were identified, which explained a relatively high variance at the P-tau level. In addition, 331 AD-related genes were identified, 10 gene–gene interaction pairs were replicated in the PPI network. The identified gene-gene interactions and genes showed associations with AD in terms of neuroinflammation and neurodegeneration, neuronal cells activation and brain development, thereby leading to cognitive decline in AD, which is indirectly associated with the P-tau pathological feature of AD and in turn supports the results of this study. Thus, the results of our study might be beneficial for explaining part of the “missing heritability” of AD.
A Porcine DNMT1 Variant: Molecular Cloning and Generation of Specific Polyclonal Antibody
Authors: Zhu Lin , Wang Jiayun , Zhang Yanbing , Xiang Xiao , Liu Ke , Wei Jianchao , Li Zongjie , Shao Donghua , Li Beibei , Ma Zhiyong , Qiu Yafeng
Published: June 2023

DNA methyltransferase 1 (DNMT1), the first-identified DNA methyltransferase in mammals, has been well studied in the control of embryo development and somatic homeostasis in mice and humans. Accumulating reports have demonstrated that DNMT1 plays an important role in the regulation of differentiation and the activation of immune cells. However, little is known about the effects of porcine DNMT1 on such functional regulation, especially the regulation of the biological functions of immune cells. In this study, we report the cloning of DNMT1 (4833 bp in length) from porcine alveolar macrophages (PAMs). According to the sequence of the cloned DNMT1 gene, the deduced protein sequence contains a total of 1611 amino acids with a 2 amino acid insertion, a 1 amino acid deletion, and 12 single amino acid mutations in comparison to the reported DNMT1 protein. A polyclonal antibody based on a synthetic peptide was generated to study the expression of the porcine DNMT1. The polyclonal antibody only recognized the cloned porcine DNMT1 and not the previously reported protein due to a single amino acid difference in the antigenic peptide region. However, the polyclonal antibody recognized the endogenous DNMT1 in several porcine cells (PAM, PK15, ST, and PIEC) and the cells of other species (HEK-293T, Marc-145, MDBK, and MDCK cells). Moreover, our results demonstrated that all the detected tissues of piglet express DNMT1, which is the same as that in porcine alveolar macrophages. In summary, we have identified a porcine DNMT1 variant with sequence and expression analyses.
Hypertrophic Cardiomyopathy Complicated by Post-COVID-19 Myopericarditis in Patient with ANO5-Related Distal Myopathy
Authors: Blagova Olga , Lutokhina Yulia , Vukolova Marina , Pirozhkov Sergey , Sarkisova Natalia , Ainetdinova Dilara , Das Anushree , Krot Marina , Smolyannikova Vera , Litvitsky Petr , Zaklyazminskaya Elena , Kogan Evgeniya
Published: June 2023

A 60-year-old male with hypertrophic cardiomyopathy, conduction disorders, post-COVID-19 myopericarditis and heart failure was admitted to the hospital’s cardiology department. Blood tests revealed an increase in CPK activity, troponin T elevation and high titers of anticardiac antibodies. Whole exome sequencing showed the presence of the pathogenic variant NM_213599:c.2272C>T of the ANO5 gene. Results of the skeletal muscle biopsy excluded the diagnosis of systemic amyloidosis. Microscopy of the muscle fragment demonstrated sclerosis of the perimysium, moderate lymphoid infiltration, sclerosis of the microvessels, dystrophic changes and a lack of cross striations in the muscle fibers. Hypertrophy of the LV with a low contractile ability, atrial fibrillation, weakness of the distal skeletal muscles and increased plasma CPK activity and the results of the skeletal muscle biopsy suggested a diagnosis of a late form of distal myopathy (Miyoshi-like distal myopathy, MMD3). Post-COVID-19 myopericarditis, for which genetically modified myocardium could serve as a favorable background, caused heart failure decompensation.
Vitamin B12 Auxotrophy in Isolates from the Deep Subsurface of the Iberian Pyrite Belt
Authors: Mateos Guillermo , Martínez-Bonilla Adrián , Martínez José M. , Amils Ricardo
Published: June 2023

Vitamin B12 is an enzymatic cofactor that is essential for both eukaryotes and prokaryotes. The development of life in extreme environments depends on cofactors such as vitamin B12 as well. The genomes of twelve microorganisms isolated from the deep subsurface of the Iberian Pyrite Belt have been analyzed in search of enzymatic activities that require vitamin B12 or are involved in its synthesis and import. Results have revealed that vitamin B12 is needed by these microorganisms for several essential enzymes such as ribonucleotide reductase, methionine synthase and epoxyqueosine reductase. Isolate Desulfosporosinus sp. DEEP is the only analyzed genome that holds a set core of proteins that could lead to the production of vitamin B12. The rest are dependent on obtaining it from the subsurface oligotrophic environment in which they grow. Sought proteins involved in the import of vitamin B12 are not widespread in the sample. The dependence found in the genomes of these microorganisms is supported by the production of vitamin B12 by microorganisms such as Desulfosporosinus sp. DEEP, showing that the operation of deep subsurface biogeochemical cycles is dependent on cofactors such as vitamin B12.
Risk of Lymphedema and Death after Lymph Node Dissection with Neoadjuvant and Adjuvant Treatments in Patients with Breast Cancer: An Eight-Year Nationwide Cohort Study
Authors: Lee Ye-Seul , Lim Yu-Cheol , Yeo Jiyoon , Kim Song-Yi , Lee Yoon Jae , Ha In-Hyuk
Published: June 2023

Knowledge on the impact of neoadjuvant and adjuvant treatments on post-surgery lymphedema (LE) in patients with breast cancer is limited due to methodological limitations and an insufficient sample size. We investigated the risk of LE in patients going through long-term anticancer treatment regimens using a national cohort from the Korean National Health Insurance Service database from 2011–2013. Incidence rate ratio, Kaplan–Meier analysis, and Cox proportional regression analysis were performed. A total of 39,791 patients were included. While minimal lymph node dissection (SLNB) reduced the risk of LE (hazard ratio [HR] 0.51) as expected, neoadjuvant chemotherapy (NAC) followed by SLNB did not reduce the risk. Adjusting for adjuvant chemotherapy (AC) as time-varying exposure decreased the risk of LE in the SLNB group (HR 0.51), but not the mortality risk (HR 0.861). A longer duration of NAC, especially taxane-based, combined with SLNB reversed the effect and increased risk of LE. The findings highlight the importance of not only early surveillance before and after surgery, but also long-term surveillance during adjuvant treatment by surgeons and oncologists in order to reduce the risk of LE.
Effects of Diamond Steps Exercises on Balance Improvement in Healthy Young and Older Adults: A Protocol Proposal
Authors: Shao Shuangyan , Mitsutake Tsubasa , Maruyama Hitoshi
Published: June 2023

Diamond step (DS) exercises are associated with multiple components of postural control and, thus, have the potential to efficiently improve balance ability. This study aimed to verify whether DS exercises contribute to improving balance ability. This study included 35 healthy young people and 29 older adults. DS exercises were performed continuously for 3 min, four times a week, for 1 month. Balance ability was assessed at baseline and after 1 and 2 months; eight items in total were examined: 30 s chair stand test, functional reach test, standing on one leg with eyes closed, time required for five rounds of DS, left–right DS, Y balance test, open–close stepping test, and finger-to-floor distance. The difficulty, achievement, and lightness/enjoyment of DS exercises were measured after the first practice and 1 month after beginning the exercises as subjective evaluations. Older adults showed improvement in seven of the eight items, with the exception being the one-legged stance with closed eyes. The subjective evaluation showed a decrease in the level of difficulty of DS exercises for older adults. DS exercises may improve balance by effectively utilizing various postural control strategies. These exercises can be effective and easy to implement, given their moderate difficulty level and self-efficacy.
Factors Affecting the Sexual Behavior of Korean University Students
Authors: Cho Mi-Kyoung , Kim Mi Young
Published: June 2023

Recent incidents in Room n, sexual harassment by social leaders, and the #MeToo movement showed Korea’s immature and distorted sexual culture. This cross-sectional descriptive study investigated the factors affecting the sexual behavior of Korean university students. The participants comprised 258 university students from S and C. The data collection period was from 29 November 2021 to 3 December 2021, and an online survey was conducted on sexual behavior, sexual attitudes, and subject characteristics. The collected data were analyzed using PASW Statistics 25.0. The average age of the participants was 21.38 ± 1.62 years old; the average age when they first watched a pornographic video on YouTube was 14.25 ± 2.55 years old. Sexual behavior was statistically significantly higher for men over 21 and under 14 when they first watched a pornographic video. As the age of the subjects increased, the younger the age of viewing pornographic videos and the thumbnail viewing path of the pornographic videos affected sexual behavior, with an explanatory power of 11.0% (F = 6.27, p < 0.001). Higher sexual attitudes in the communion and permissiveness domains showed greater influence on sexual behavior; the explanatory power was 24.0% (F = 10.02, p < 0.001). Korean university students must be educated on sex early to develop correct sexual attitudes and engage in correct and responsible sexual behaviors in their youth.
Mindfulness, Self-Compassion, and Acceptance as Predictors of Sexual Satisfaction in Cisgender Heterosexual Men and Women
Authors: Peixoto Maria Manuela
Published: June 2023

Sexual satisfaction is a relevant indicator of sexual health, and psychotherapeutic interventions for sexual dysfunction also promote sexual satisfaction in men and women. Cognitive-behavioral psychotherapies for sexual dysfunction, including third-wave approaches, are effective in treating sexual dysfunction. Thus, third-wave cognitive-behavioral constructs may play a significant role in sexual satisfaction. This study intends to examine the predictive role of mindfulness awareness and attention, self-compassion and acceptance, and action constructs on cisgender heterosexual men’s and women’s sexual satisfaction. A web survey including self-report measures for assessing mindfulness awareness and attention (MAAS), self-compassion (SCS), acceptance and action (AAQ), and sexual satisfaction (GMSEX) was disseminated during 2022, and a sample of 420 participants was collected (n = 238 women; 56.7%; n = 182 men; 43.3%). No statistically significant differences were found between cisgender heterosexual men and women on mindfulness awareness and attention, self-compassion, acceptance and action, and sexual satisfaction. In addition, all variables account for 6.5% of cisgender heterosexual men’s and women’s sexual satisfaction variance, and mindfulness awareness and attention, self-compassion, and acceptance and action positively predicted sexual satisfaction. Overall, mindfulness awareness and attention, self-compassion, and acceptance and action play a significant predictive role in cisgender heterosexual men’s and women’s sexual satisfaction.
Community Pharmacies’ Promotion of Smoking Cessation Support Services in Saudi Arabia: Examining Current Practice and Barriers
Authors: Alzahrani Fahad , Sandaqji Yazeed , Alharrah Abdullah , Alblowi Ramzi , Alrehaili Samer , Mohammed-Saeid Waleed
Published: June 2023

Background: Community pharmacists’ interventions have been found to be highly effective. However, to date, there has been little information about pharmacists’ potential roles and perceived barriers to providing smoking cessation. This study aimed to assess the potential role of community pharmacists in supporting their patients to quit smoking by summarizing their self-reported level of current activities, confidence, and perceived barriers; Methods: A self-administered questionnaire was used for a cross-sectional study in Saudi Arabia. As part of the questionnaire, demographic characteristics were measured, as well as confidence, perceived berries, and level of smoking cessation activities (asking, advising, assessing, assisting, and arranging, including follow-up).; Results: 370 community pharmacists practicing responded to the survey. Pharmacists indicated high activity rates about advising and assessing patients in quitting smoking, with lower rates of assisting and arranging, including following up. The rate of recording smoking status was very low. There were significant differences between gender, source of education, years practicing as a licensed pharmacist, attended an education or training program on smoking cessation, and interest in providing smoking and perceived practice of smoking cessation. Pharmacists are confident about providing smoking cessation activities. Barriers to providing smoking cessation services include unable to follow up, lack of counseling space, lack of educational materials, and lack of time.; Conclusions: Saudi Arabian community pharmacists actively offer smoking cessation services, which may reduce smoking-related health issues. It appears that pharmacists need to be empowered by team-based, systematic, comprehensive approaches to overcome barriers and enhance their confidence.
Effects of Rearfoot Eversion on Foot Plantar Pressure and Spatiotemporal Gait Parameters in Adolescent Athletes
Authors: Fujishita Hironori , Ikuta Yasunari , Maeda Noriaki , Komiya Makoto , Morikawa Masanori , Arima Satoshi , Sakamitsu Tetsuhiko , Obayashi Hiromune , Fukuhara Kouki , Ushio Kai , Adachi Nobuo
Published: June 2023

Background: Foot malalignment can augment the risk of lower-extremity injuries and lead to musculoskeletal disorders. This study aimed to clarify the contribution of rearfoot alignment to plantar pressure distribution and spatiotemporal parameters during gait in healthy adolescent athletes. Methods: This retrospective study included 39 adolescent athletes who were divided into the rearfoot eversion and control groups according to a leg heel angle of 7°. A total of 78 legs were analyzed (45 and 33 legs in the rearfoot eversion [women, 53.3%] and control groups [women, 48.5%], respectively). Gait was assessed using an in-shoe plantar pressure measuring system and a wearable inertial sensor. Results: The foot plantar pressure distribution in the hallux was higher in the rearfoot eversion group than that in the control group (p = 0.034). Spatiotemporal parameters showed that the foot pitch angle at heel strike was significantly larger in the rearfoot eversion group than that in the control group (24.5° vs. 21.7°; p = 0.015). Total sagittal range of motion of the ankle during the stance phase of gait was significantly larger in the rearfoot eversion group than that in the control group (102.5 ± 7.1° vs. 95.6 ± 15.8°; p = 0.020). Logistic regression analysis revealed that plantar pressure at the hallux and medial heel and foot pitch angle at heel strike were significantly associated with rearfoot eversion. Conclusions: Our findings suggest that rearfoot eversion affects the gait patterns of adolescent athletes. Notably, leg heel angle assessment, which is a simple and quick procedure, should be considered as an alternative screening tool for estimating plantar pressure and spatiotemporal gait parameters to prevent sports-related and overuse injuries in adolescent athletes.
New Insight into Liu Kang’s Village Scene (1931): A Non-Invasive Investigation by Technical Imaging
Authors: Lizun Damian
Published: June 2023

This study examines the intriguing peculiarities of the surface paint layer found in the painting Village scene (1931) by renowned Singapore artist Liu Kang (1911–2004). The incorporation of non-invasive visible light (VIS) and near-infrared (NIR) photography techniques, combined with high-power digital microscopy, revealed unusual features on the surface paint layer. Flattened impastos, clusters of incrusted foreign paint unrelated to the existing paint scheme, and fragments of paper with printed traditional Chinese characters were identified on the painting’s surface. The results of the analyses cross-referenced with the archival photographs enabled the consideration of the specified features of the paint layer as unintentional damage caused by the artist due to inadequate storage and transportation conditions—paradoxically, in his attempt to protect the painting. As these damaged areas pose potential display and conservation problems, three conservation strategies were proposed based on ethical guidelines formulated by various governing bodies for the conservation profession. This study demonstrates that there is no universal conservation solution that can satisfy conflicting aesthetic and ethical opinions. The damage to the paint layer affects the visual properties of the artwork but also provides evidence of its complex history. In light of the above, there may be valid arguments both for returning the painting to its original state and for preserving its current condition. Therefore, good practice would require balanced judgments from conservators and curators, considering Village scene in the broader context of Liu Kang’s early painting practice and the existing archival information about the artist.
Specific Design Approach of Croatian Architect Dinko Kovačić: The Coexistence of Modernism and Tradition in the Second Half of the 20th Century
Authors: Perković Jović Vesna , Mrinjek Kliska Neda
Published: June 2023

Dinko Kovačić is a prominent Croatian architect and university professor. His design approach is characterised by exceptional empathy that results in architectural works of intense connection with the environment as well as with that of their future users. Although many of Kovačić’s works have been published in the daily press and professional publications, the complete oeuvre of this architect has so far not been the subject of scientific research. The aim of this scientific work is to look at his specific design approach based on the analysis of representative examples. Research methods in this paper include the analysis of primary and secondary sources and on-site observation. The article gives a systematic presentation of Dinko Kovačić’s work as related to his specific approach, which integrates the modernism of the second half of the 20th century and the Mediterranean tradition.
Identification of a Locus Controlling Seed Pigment Leaching in Cowpea [Vigna unguiculata (L.) Walp]
Authors: Bowman Christian S. , Huynh Bao Lam , Roberts Philip , Santos Jansen R. P. , Paul Kaylee , Close Timothy J.
Published: June 2023

Consumer preferences for cooking-related traits are a deciding factor in the success of new cowpea [Vigna unguiculata (L.) Walp] cultivars. Pigment leaching is an undesirable trait for both consumers and producers alike that occurs during the cooking or canning process and has been a goal for improvement efforts through breeding. This study leverages the power of bulked segregant analysis to identify a locus segregating for the pigment-leaching trait in an F2 population of blackeye seed-type cowpea. A single major locus was identified on Vu06 spanning 1.27 Mb, and SNP haplotypes were identified for low and high pigment-leaching bulks. However, further evaluation of accessions that are unrelated to the F2 population or its progenitors suggests that the trait is polygenic, such that low or high leakage is not determined solely by this locus. Parallels were drawn between cowpea and a close relative, black bean (Phaseolus vulgaris L.), to suggest that additional seed coat or cooking-related traits may also be involved in the pigment-leaching trait.
Benefit Information’s Impact on Ornamental Plant Value
Authors: Rihn Alicia L. , Knuth Melinda J. , Behe Bridget K. , Hall Charles R.
Published: June 2023

Ornamental plants provide many benefits to humans, including those that affect emotional/mental health, physiological health, social interactions, and economic/financial conditions. However, research related to consumers’ perceived value of ornamental plants has focused primarily on production methods that were used to produce plants, while consumers’ perceived intrinsic value of postpurchase benefits has not been addressed. There is potential for benefit information to generate value for consumers. Knowing consumers’ value for different plant benefits serves to inform green industry stakeholders as they consider their marketing strategies and whether to include benefit information as a part of their plant promotions. An online survey was conducted to elicit U.S. consumers’ willingness to pay (WTP) for lavender plants while displaying different plant benefit information. Participants randomly viewed one of eight plant benefit messages and indicated the maximum they were willing to pay for a lavender plant displaying that benefit. The benefits messages were categorized as cognitive, social, physical, emotional, environmental, aesthetic, educational, and economic based on prior literature. Tobit models were used to analyze the data. Compared to economic benefit information, the social benefit generated the largest premium (USD 6.75). Plant spending in 2020 and household income increased participants’ WTP for the lavender plants. Age had a negative impact on WTP.
Plant Physiological Assessments on Promising New HLB-Tolerant Citrus Rootstocks after Inoculation with the Phytopathogenic Ascomycete Rosellinia necatrix
Authors: Arjona-López Juan , Gmitter Frederick , Romero-Rodríguez Estefanía , Grosser Jude , Cantero-Sánchez José , López-Herrera Carlos , Arenas-Arenas Francisco
Published: June 2023

Citriculture is considered the most important fruit industry and involves the cultivation of several fruit varieties, which are susceptible to many plant pathogens. In this sense, soil-borne pathogenic fungi, such Rosellinia necatrix, threaten citrus fruit production worldwide because they can cause fruit loss. Therefore, we assayed the physiological reaction of novel citrus rootstocks against white root rot disease during long-term management. Data from above-ground symptoms and chlorophyll content were periodically obtained during the experimental process. In addition, plant leaf area and percentage of biomass reduction were determined for each rootstock when the experiment finished. The behavior of the inoculated tolerant rootstocks was as follows: the lowest symptom rate of SAUDPC was achieved by B11R5T25 and N40R3T25; AMB+CZO manifested the highest disease incidence; B11R5T25 and A+VOLK × Orange 19-11-8 displayed the highest and the lowest chlorophyll content, respectively; AMB+CZO and A+VOLK × Orange 19-11-8 showed the highest biomass reduction, and the lowest was detected in B11R5T25 and N40R2T19; concerning the leaf area, N40R1T18, N40R3T25 and N40R2T19 showed the lowest response, and 2247 × 2075-01-2 achieved the highest rate. In summary, B11R5T25 and N40R3T25 displayed the lowest disease rate.
Access to Information and Communication Technology, Digital Skills, and Perceived Well-Being among Older Adults in Hong Kong
Authors: Fung Kwok-kin , Hung Shirley Suet-lin , Lai Daniel W. L. , Shum Michelle H. Y. , Fung Hong-wang , He Langjie
Published: June 2023

Population aging is a major concern worldwide. Active aging should be promoted by increasing the social participation of older adults and enabling them to remain involved in the community. Research has demonstrated the utility of digital resources for addressing the needs of older adults, which include networking, entertaining, and seeking health-related information. However, the digital divide among older adults (i.e., the “gray digital divide”) is increasingly being recognized as a social problem that may be related to poor well-being. To obtain updated local data on the prevalence of Internet access and usage and their relationship with perceived well-being, we conducted telephone interviews with a random sample of 1018 older adults in Hong Kong from January to July 2021 (This research has received funding support from the Interdisciplinary Research Matching Scheme, Hong Kong Baptist University). We found that only 76.5% of the participants had Internet access at home, a mobile phone data plan, or both, whereas 36.3% had never used Internet services and 18.2% had no digital devices. A younger age, male gender, higher education level, living with others, and higher self-perceived social class were associated with material access to digital devices and everyday use of Internet services. Participants who accessed the Internet every day had higher levels of life satisfaction and self-rated physical and mental health than those who rarely or never used the Internet. Hierarchical multiple regression analysis revealed that digital skills were significantly associated with self-rated mental health even when controlling for demographic variables (including age, gender, education level, and self-perceived social class). This study contributes to the limited body of literature on the relationship between Internet use, digital skills, and perceived well-being. Our findings highlight the importance of addressing the digital divide even in cities with high penetration of information and communication technology, such as Hong Kong. We also discuss our insights into the use of timely interventions for older adults to address the gray digital divide.
Global Health Perspectives on Race in Research: Neocolonial Extraction and Local Marginalization
Authors: Tankwanchi Akhenaten Siankam , Asabor Emmanuella N. , Vermund Sten H.
Published: June 2023

Best practices in global health training prioritize leadership and engagement from investigators from low- and middle-income countries (LMICs), along with conscientious community consultation and research that benefits local participants and autochthonous communities. However, well into the 20th century, international research and clinical care remain rife with paternalism, extractive practices, and racist ideation, with race presumed to explain vulnerability or protection from various diseases, despite scientific evidence for far more precise mechanisms for infectious disease. We highlight experiences in global research on health and illness among indigenous populations in LMICs, seeking to clarify what is both scientifically essential and ethically desirable in research with human subjects; we apply a critical view towards race and racism as historically distorting elements that must be acknowledged and overcome.
Changes in Quantity Measures of Various Forms of Cannabis Consumption among Emerging Adults in Canada in Relation to Policy and Public Health Developments
Authors: Yousufzai Susan J. , Cole Adam G. , Nonoyama Mika , Barakat Caroline
Published: June 2023

Limited research examines changes in quantities of various forms of smoked/vaped cannabis among regular consumers, including emerging adults (EAs; 18 to 29) in Canada. This information is particularly relevant in the current context of emerging cannabis behaviors among EAs related to political amendments (legalization of cannabis), vaping-related lung illnesses (EVALI), and unprecedented pandemics (COVID-19). This study investigated the impact of legalizing recreational cannabis use in Canada, the EVALI epidemic, and the COVID-19 pandemic on the quantity of smoked/vaped forms of cannabis in relation to gender differences. EAs retrospectively self-reported the quantity of herb, hash, concentrates, joint size, and the number of joints and vaping cartridges in relation to three consecutive developments: pre-legalization, post-legalization; pre-EVALI, post-EVALI, pre-COVID-19, and during COVID-19. The quantity of herb use significantly increased among heavy users, and vaping quantity significantly increased among light users. Overall, an increasing incremental trend was observed in the average quantity of cannabis forms used over time. Males consumed higher quantities of all cannabis forms than females. More males than females reported using concentrates (p < 0.05). These findings reveal unique aspects of the amount of various cannabis forms smoked/vaped in relation to gender and provides preliminary evidence of cannabis consumption behaviors in relation to changing social and cultural contexts.