bluuebunny/crossref_metadata_2025 huggingface dataset:
Long Non-Coding RNA H19 Prevents Lens Fibrosis through Maintaining Lens Epithelial Cell Phenotypes
Authors: Xiong Lang , Sun Yan , Huang Jingqi , Ma Pengjuan , Wang Xiaoran , Wang Jiani , Chen Baoxin , Chen Jieping , Huang Mi , Huang Shan , Liu Yizhi
Published: August 2022

The integrity of lens epithelial cells (LECs) lays the foundation for lens function and transparency. By contrast, epithelial-mesenchymal transition (EMT) of LECs leads to lens fibrosis, such as anterior subcapsular cataracts (ASC) and fibrotic forms of posterior capsule opacification (PCO). However, the underlying mechanisms remain unclear. Here, we aimed to explore the role of long non-coding RNA (lncRNA) H19 in regulating TGF-β2-induced EMT during lens fibrosis, revealing a novel lncRNA-based regulatory mechanism. In this work, we identified that lncRNA H19 was highly expressed in LECs, but downregulated by exposure to TGF-β2. In both human lens epithelial explants and SRA01/04 cells, knockdown of H19 aggravated TGF-β2-induced EMT, while overexpressing H19 partially reversed EMT and restored lens epithelial phenotypes. Semi-in vivo whole lens culture and H19 knockout mice demonstrated the indispensable role of H19 in sustaining lens clarity through maintaining LEC features. Bioinformatic analyses further implied a potential H19-centered regulatory mechanism via Smad-dependent pathways, confirmed by in vitro experiments. In conclusion, we uncovered a novel role of H19 in inhibiting TGF-β2-induced EMT of the lens by suppressing Smad-dependent signaling, providing potential therapeutic targets for treating lens fibrosis.
A Comparison of Clinical Features of Youth with and without Rhinitis Signs and Symptoms Who Are Hospitalized for Headache
Authors: Tang Si-Jia , Lee Heejin , Cui Tiantian , Lee Jae Min , Ahn Ji Young , Lee Sua , Kim Saeyoon
Published: August 2022

Headache and allergic rhinitis (AR) are common in children and often co-occur. We investigated the clinical characteristics of pediatric headaches and the association of AR and chronic headaches. We retrospectively reviewed the medical records of patients admitted to our pediatric inpatient and outpatient clinics with complaints of headache between January 2017 and June 2020 for headache-specific history, AR signs and symptoms, allergy skin prick test, inhalant multiple allergen simultaneous test results, laboratory and imaging findings, and medication history. The patients were divided into three subgroups: AR, non-AR, and headache groups, reporting 45.7% patients with headache alone, 13.7% with additional AR, and 31.6% with abnormal imaging findings, suggesting that headache was combined with sinusitis (24.3%) or mastoiditis (7.3%). Furthermore, 6% of the patients had both AR and sinusitis. Body mass index (BMI) differed significantly between the AR and the non-AR and headache groups (p = 0.03). The BMI differed significantly according to headache severity (p ˂ 0.001). The most common allergen was “dust or mites” (41.1%). Acetaminophen (35.9%) was the most commonly used painkiller. The coexistence of AR and headache may indicate that these conditions share a similar pathophysiology. Better management of allergies may facilitate diagnosis, treatment, and prophylaxis of headaches.
Pediatric Open Long-Bone Fracture and Subsequent Deep Infection Risk: The Importance of Early Hospital Care
Authors: Kuhn Andrew W. , Troyer Stockton C. , Martus Jeffrey E.
Published: August 2022

The purpose of the current study was to identify risk factors for deep infection after an open long-bone fracture in pediatric patients. Systematic billing queries were utilized to identify pediatric patients who presented to a level I trauma center from 1998 to 2019 with open long-bone fractures. There were 303 open long-bone fractures, and 24 (7.9%) of these became infected. Fractures of the tibia/fibula (p = 0.022), higher revised Gustilo-Anderson type (p = 0.017), and a longer duration of time between the injury and hospital presentation (p = 0.008) were all associated with the presence of deep infection. Those who went on to have a deep infection also required more operative debridements (p = 0.022) and a total number of operative procedures (p = 0.026). The only factor that remained significant in multivariable regression was the duration between the injury and hospital presentation (OR 1.01 [95%CI 1.003–1.017]; p = 0.009), where the odds of deep infection increased by 1% for every minute of delayed presentation.
The Role of the Compressor Isentropic Efficiency in Non-Intrusive Refrigerant Side Characterization of Transcritical CO2 Heat Pump Water Heaters
Authors: Lamas Francisco B. , Costa Vítor A. F.
Published: August 2022

Characterizing the refrigerant side of heat pump water heaters (HPWHs) can be intrusive and expensive. On the other hand, direct external measurement techniques can be unfeasible, particularly in commercial HPWHs for residential applications. Non-intrusive in situ characterization methods have already been successfully implemented in subcritical heat pumps. They provide the refrigerant mass flowrate and the equipment energy performance, by using contact temperature sensors and electric power meters. Subcritical suction and discharge-specific enthalpies necessary to apply the method can be obtained from the measured temperatures and their corresponding saturation pressures. Nevertheless, this approach does not apply to the transcritical CO2 HPWHs. In the supercritical region, temperature and pressure are independent variables, and an iterative process regarding the compressor isentropic efficiency has to be considered. However, when isentropic efficiency data are not available, an additional procedure is required, using a validated gas cooler model to verify the physical reliability of the numerical solutions. This work aims at presenting base thermodynamic analysis of a novel methodology for non-intrusive refrigerant side characterization of transcritical CO2 HPWHs, exploring the influence of the compressor isentropic efficiency condition.
Novel Terahertz Properties of Nanostructured Mn3+0.53Sn Films with Different Crystalline Orientations Driven by Ostwald Ripening on (0001) c-Al2O3
Authors: Yang Shaopeng , Wang Xinyue , Wang Qiujin , Xu Tong , Wang Qian , Guo Jin , Zuo Jian , Zhang Cunlin , Xu Wen , Song Yujun , Liu Ying , Wang Jing , Wang Hai
Published: August 2022

The characteristic energies of elementary excitations and collective modes in many quantum materials lie mostly in the terahertz (THz) frequency range, which provides a wide space for the development of THz optical materials and devices. In particular, topological Weyl semimetal Mn3Sn is a noncollinear antiferromagnet with anomalous THz properties, which is strongly affected by thermal energy and external magnetic fields. Despite the explosive growth of the research on magnetic Weyl semimetals recently, its nanoscale structure for applications in THz optical devices remains to be explored. Here, we fabricated nanostructured Mn3+0.53Sn films with different crystal orientations, driven by Ostwald Ripening (OR) on (0001) c-Al2O3. A huge anisotropic THz response manifested a firm link between the optical properties of Weyl antiferromagnet Mn3Sn and its contrivable spin structures. The topological properties of Mn3Sn are robustly protected in its nanostructures. This work can provide a new horizon for the fabrication of a nanostructured magnetic Weyl alloy and for its potential applications in subwavelength high-performance THz devices.
Fabrication and Characterization of P-Type Semiconducting Copper Oxide-Based Thin-Film Photoelectrodes for Solar Water Splitting
Authors: Chang Chih-Jui , Lai Chih-Wei , Jiang Wei-Cheng , Li Yi-Syuan , Choi Changsik , Yu Hsin-Chieh , Chen Shean-Jen , Choi YongMan
Published: August 2022

Solar light-driven hydrogen by photocatalytic water splitting over a semiconductor photoelectrode has been considered a promising green energy carrier. P-type semiconducting copper oxides (Cu2O and CuO) have attracted remarkable attention as an efficient photocathode for photoelectrochemical (PEC) water splitting because of their high solar absorptivity and optical band gaps. In this study, CuO thin films were prepared using the sol-gel spin coating method to investigate the effects of aging time and layer dependency. Electrodeposition was also applied to fabricate Cu2O thin films. Cu2O thin films annealed at 300 °C are a hetero-phase system composed of Cu2O and CuO, while those at 400 °C are fully oxidized to CuO. Thin films are characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), ultraviolet-visible spectroscopy (UV-VIS), Fourier transform infrared spectroscopy (FTIR), spectroscopic ellipsometry (SE), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Raman microscopy. The hetero-phase thin films increase the photoconversion efficiency compared to Cu2O. Fully oxidized thin films annealed at 400 °C exhibit a higher efficiency than the hetero-phase thin film. We also verified that CuO thin films fabricated using electrodeposition show slightly higher efficiency than the spin coating method. The highest photocurrent of 1.1 mA/cm2 at 0.10 V versus RHE was measured for the fully oxidized CuO thin film under one-sun AM1.5G illumination. This study demonstrates a practical method to fabricate durable thin films with efficient optical and photocatalytic properties.
Analysis of Smoluchowski’s Coagulation Equation with Injection
Authors: Makoveeva Eugenya V. , Alexandrov Dmitri V. , Fedotov Sergei P.
Published: August 2022

The stationary solution of Smoluchowski’s coagulation equation with injection is found analytically with different exponentially decaying source terms. The latter involve a factor in the form of a power law function that plays a decisive role in forming the steady-state particle distribution shape. An unsteady analytical solution to the coagulation equation is obtained for the exponentially decaying initial distribution without injection. An approximate unsteady solution is constructed by stitching the initial and final (steady-state) distributions. The obtained solutions are in good agreement with experimental data for the distributions of endocytosed low-density lipoproteins.
Conservation Significance of the Rare and Endangered Tree Species, Trigonobalanus doichangensis (Fagaceae)
Authors: Hu Ling , Le Xin-Gui , Zhou Shi-Shun , Zhang Can-Yu , Tan Yun-Hong , Ren Qiang , Meng Hong-Hu , Cun Yupeng , Li Jie
Published: August 2022

Trigonobalanus doichangensis is a rare and endangered species with important evolutionary value and extremely small populations. We investigated the genetic diversity of T. doichangensis to provide information on its effective preservation. We used genotyping-by-sequencing (GBS) technology to assess the genetic diversity, genetic structure and gene flow of the six populations of T. doichangensis. Analysis of SNPs indicated that there was high genetic diversity in the ML and XSBN populations of T. doichangensis. FST values showed moderate genetic differentiation among the populations of T. doichangensis. Meanwhile, admixture, principal components and gene flow analyses indicated that the populations of T. doichangensis are not genetically separated in accordance with their geographical distributions. Habitat destruction and excessive exploitation may have led to a low gene flow, which has in turn resulted in the differences in seed and seedling morphological traits among populations. Based on these findings, we recommend that T. doichangensis be conserved through in situ approaches and artificial seedlings, including preservation of each extant population. Particularly, the ML and XSBN populations have high diversity and more ancestral information, so these two populations should be considered as conservation priorities, and seeds should be collected to obtain germplasm and increase the genetic diversity.
Diversity of Seed Flavan-3-Ols in Croatian Native Grapevine Cultivars (Vitis vinifera L.) Grown in Coastal Region
Authors: Andabaka Željko , Šikuten Iva , Tomaz Ivana , Stupić Domagoj , Marković Zvjezdana , Kontić Jasminka Karoglan , Maletić Edi , Preiner Darko
Published: August 2022

Seed extracts are becoming more important due to their beneficial biological activities. The main constituents of seed extracts are flavan-3-ols, compounds important in winemaking. The coastal region in Croatia is rich in native grapevine varieties, which are used in wine production. The aim of the research was to analyze the flavan-3-ol profiles of 20 native varieties, and to evaluate the potential use of grape seeds as a source of flavan-3-ols. The flavan-3-ols from seeds were analyzed by HPLC. The predicted yield of flavan-3-ols was calculated using the analyzed profiles. In total, eight compounds were identified, with the most abundant compounds being catechin, epicatechin, and procyanidin B2. In general, the red grape varieties had higher content of flavan-3-ols than the white varieties, which was confirmed by PCA. The coastal region could potentially yield up to 73.97 kg/ha of flavan-3-ols, depending on the variety. The results show the diversity of flavan-3-ol profiles among Croatian varieties and their potential usage as a source of valuable nutraceuticals.
Effects of Wood Distillate on Seedling Emergence and First-Stage Growth in Five Threatened Arable Plants
Authors: Fanfarillo Emanuele , Fedeli Riccardo , Fiaschi Tiberio , de Simone Leopoldo , Vannini Andrea , Angiolini Claudia , Loppi Stefano , Maccherini Simona
Published: August 2022

Wood distillate (WD) is an environmentally safe bio-based product stimulating plant growth and yield and allowed in Italy in organic farming. To the best of our knowledge, there are no studies on the effects of WD on spontaneous plants growing among crops, including their functional traits such as biomass. To test such effects, we carried out a lab experiment on artificially reconstructed arable plant communities composed of five species of conservation interest, which are specialist winter cereal crops: Bromus secalinus L., Centaurea cyanus L., Lathyrus aphaca L., Legousia speculum-veneris (L.) Chaix, and Scandix pecten-veneris L. After sowing 45 pots under controlled conditions, we applied WD at three concentrations (0%, 0.2%, and 0.5%) six times over 7 weeks. The number of emerged plants in each pot was counted every two weeks. Finally, we harvested all plants and measured the fresh and dry above-ground weight of each species in each pot. The resulting data were analyzed by Permutational Analysis of Variance. The application of 0.2% and 0.5% WD modified the community composition after two weeks, but such differences later disappeared. Both 0.2% and 0.5% WD had a positive effect on the dry weight of S. pecten-veneris and a negative effect on that of L. speculum-veneris. Moreover, 0.2% and 0.5% WD increased seedling emergence in L. aphaca, and 0.5% WD increased seedling emergence in S. pecten-veneris. Both 0.2% and 0.5% WD enhanced seedling emergence in the entire community. We suggest that the use of WD at low concentrations in winter cereals may be a sustainable agricultural practice that benefits crops without harming the associated plant diversity.
Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads
Authors: Bae Minju , Park Junsoo , Seong Hyeonah , Lee Hansol , Choi Wonsuk , Noh Jiyun , Kim Woojoo , Shin Sehyun
Published: August 2022

The complex and lengthy protocol of current viral nucleic acid extraction processes limits their use outside laboratory settings. Here, we describe a rapid and reliable method for extracting nucleic acids from viral samples using a rotating blade and magnetic beads. The viral membrane can be instantly lysed using a high-speed rotating blade, and nucleic acids can be immediately isolated using a silica magnetic surface. The process was completed within 60 s by this method. Routine washing and eluting processes were subsequently conducted within 5 min. The results achieved by this method were comparable to those of a commercially available method. When the blade-based lysis and magnetic bead adsorption processes were performed separately, the RNA recovery rate was very low, and the Ct value was delayed compared to simultaneous lysis and RNA adsorption. Overall, this method not only dramatically shortens the conventional extraction time but also allows for its convenient use outside the laboratory, such as at remote field sites and for point-of-care testing.
Domestic vs. External Economic Sectors and the Political Process: Insights from Greece
Authors: Petrakos George , Rontos Kostas , Salvati Luca , Vavoura Chara , Vavouras Ioannis
Published: August 2022

Building on the well-established relationship between economic dynamics and political processes, we focus on the most important element of the political process, namely, general (or national) elections, and look into their effects on public finance and total economic output. In this vein, the present study has three objectives: (i) to investigate political budget cycles in Greece during the period known as the ‘Third Hellenic Republic’ (in Greek, ‘Metapolitefsi’, hereafter THR) since 1974; (ii) to assess whether national elections affect total economic activity in a stabilizing or destabilizing way; and (iii) to examine the possible effects of the external sector of the economy on the budget balance. The empirical findings of our analysis document how the Greek economy was characterized by sharp political budget cycles in correspondence with the THR, exerting a destabilizing effect on the total output of the economy. Performances of the external sector of the economy have significantly affected budget balances in Greece.
Intra Complexity Control Algorithm for VVC
Authors: Shu Zhengjie , Li Junyi , Peng Zongju , Chen Fen , Yu Mei
Published: August 2022

Versatile Video Coding (VVC) has advantages over High Efficiency Video Coding (HEVC); it can save nearly half of the bit rate and significantly improve the compression efficiency, but VVC’s coding complexity is extremely high. Therefore, VVC encoders are difficult to implement in video devices with different computing capabilities and power constraints. In this paper, we apply texture information and propose a VVC intra complexity control algorithm. The algorithm assigns a different encoding time to each CU based on the corresponding texture entropy. Besides, the complexity reduction strategy at the CU level is designed to balance the complexity control while taking rate-distortion performance into consideration. Experiments in our paper show that the coding complexity can be accurately controlled from 90% to 70% with a slight loss of RD performance.
A Startup Control Method for Plasma Lamps by Using Fractional-N Phase-Locked Loop Microwave Driver
Authors: Xu Mengyao , Jia Hua , Huan Weiding , Shan Jiafang
Published: August 2022

The microwave-driven plasma light source has the feature of the full spectrum, similar to sunlight, and has broad application prospects. Startups of plasma lamps are very difficult since the plasma density and the resonator impedance are changing constantly with the warm-up process and the RF driver can hardly couple the power to the plasma efficiently. This paper presents a startup control method for plasma lamps by using an integrated fractional-N phase-locked loop (PLL) as the RF signal source. An RF control module including a 21 dBm RF source with a band of 430–460 MHz and detection circuits for the RF driver is developed, and the startup process of the plasma lamp is further studied by analyzing the return loss of the resonator under the plasma load. According to the test result, the maximum frequency deviation of the RF control module is 3.3 kHz over the working temperature range of −40–80 °C. The designed RF control module is used to drive a high-power amplifier to start the plasma lamp automatically. It takes 79 s to achieve the stable arc operation from the gas breakdown. The return loss of the resonator is −26 dB, with an incident power of 171 W and reflected power of 418 mW, indicating that the RF driver and the plasma achieve good coupling. Compared with continuous wave, the luminous flux of the lamp powered by RF pulse improves by 18% under the same electric power. This startup control method has stable performance, small temperature drift, and an effective control function for plasma lamps.
Numerical Investigation of the Adsorption Process of Zeolite/Water in a Thermochemical Reactor for Seasonal Heat Storage
Authors: Abohamzeh Elham , Frey Georg
Published: August 2022

Zeolite 13X molecular sieve with high sorption capacity and significant sorption rate has been considered a promising candidate for seasonal heat storage. In this study, a code is developed to simulate the adsorption process between zeolite and water in all ranges of partial pressures, temperatures, and sorbate loadings. The results from the proposed code were compared with experiments and good agreement was observed. After validation, the developed model was used to study the effective parameters involved in the adsorption process of binder-free Zeolite 13X. A parametric study considering various temperatures and water content in the inflow air was conducted and the influence of different factors on the outlet temperature and adsorption enthalpy has been studied. This parametric study gives a good insight into the measures which can be taken for achieving the desired released energy or having the outlet temperature in the preferred range. The simulations have been conducted in a variety of temperature ranges provided during the desorption process, the humidity amount, and the mass flow rate of the incoming air. The relative influence of each parameter in the specified ranges is presented. The results have demonstrated the direct relationship of the partial pressure of water vapor and the desorption temperature with the adsorbed water amount and adsorption enthalpy while changing the mass flow rate mostly influences the discharging time.
Recent Advances in Mechanochemical Pretreatment of Lignocellulosic Biomass
Authors: Pérez-Merchán Antonio Manuel , Rodríguez-Carballo Gabriela , Torres-Olea Benjamín , García-Sancho Cristina , Maireles-Torres Pedro Jesús , Mérida-Robles Josefa , Moreno-Tost Ramón
Published: August 2022

Biorefineries are industrial facilities where biomass is converted into chemicals, fuels and energy. The use of lignocellulose as raw material implies the development of pretreatments to reduce its recalcitrant character prior to the processes that lead to the synthesis of the products of interest. These treatments are based on physico-chemical processes where it is necessary to use acids, bases, oxidants, and high pressure and temperature conditions that lead to the depolymerization of lignocellulose at the expense of generating a series of streams that must be treated later or to the production of by-products. In recent years, mechanochemistry is becoming relevant in the design of processes that help in the depolymerization of lignocellulose. These mechanochemical processes are being used in combination with chemicals and/or enzymes, allowing the use of minor loads of reagents or enzymes. In this review, the advances achieved in the use of mechanochemistry for treating lignocellulosic biomass or cellulose will be presented, with special emphasis on how these mechanochemical processes modify the structure of lignocellulose and help subsequent treatments. It will focus on using ball milling or extrusion, ending with a section dedicated to future work needed to implement these technologies at the industrial level.
Repairing What Policy Is Missing Out on: A Constructive View on Prospects and Preconditions for Sustainable Biobased Economy Options to Mitigate and Adapt to Climate Change
Authors: Faaij André P. C.
Published: August 2022

Biomass use for energy and materials is, on the one hand, one of the key mitigation options to reach the 1.5 °C GMT target set in the Paris Agreement, as highlighted by the IPCC and many other key analyses. On the other hand, particularly in parts of the EU, a strong negative connotation has emerged in public debate and EC policy, with a particular emphasis on the (presumed) displacement effect in markets and land use. This is a remarkable contrast because the reasons to use sustainable biomass, on the one hand, and the possibilities and synergies for supplying sustainable biomass, on the other, are underpinned with strong evidence, also providing insights on how displacement issues can be avoided. Sustainable biomass supplies can contribute 20–30% of the future global and European energy supply, leading to reduced overall mitigation costs, including realizing the net CO2 removal from the atmosphere using BECCS concepts. This paper highlights which options, pathways and preconditions are key to achieving such a substantial contribution of sustainable biomass in future (2050) energy and material supply (with a focus on the European setting). By pinpointing how “biomass can be done right” and how important synergies can be achieved via better agricultural methods, the restoration of marginal and degraded lands and the adaptation of climate change, a different policy agenda emerges in sharp contrast to how a biobased economy has been framed in recent years. It is recommended that future policy priorities, particularly at the EU level, take a more integral view on the synergy between the role of biomass in the energy transition, climate adaptation and mitigation, better agriculture and the better use of land in general. Strategies to achieve such positive results typically require an alignment between renewable energy, and agricultural, environmental, mitigation and adaptation policies, which is a largely missing nexus in different policy arenas. Resolving this lack of alignment offers a major opportunity, globally, to contribute to the European Green deal and improve energy security.
Emission of Nitric Oxide during the Combustion of Various Forms of Solid Biofuels in a Low-Power Heating Device
Authors: Kraszkiewicz Artur , Przywara Artur , Parafiniuk Stanisław
Published: August 2022

In this study, in terms of the mechanisms of nitric oxide (NO) emissions, research was carried out to consider the impact of physical and chemical properties of wood and herbaceous biomass processed into pellets and briquettes in the course of the combustion process (in individual phases) in a low-power heating device. Combustion tests in the grate heating device showed statistically significant differences in the combustion process and thus carbon monoxide (CO), nitric oxide (NO), and sulfur dioxide (SO2) emissions in the fuel form and the combustion phase. In terms of assessing the ecological and energy parameters of the combustion process, the nitrogen content in biomass was not the most important factor indicating the formation of NO emissions. Usually, the strongest correlations were observed with the formation of NO emissions in the first phase of combustion, which was related to the emissions of CO and SO2. In the second and third flame phases, a significant reduction in NO emissions was observed, which was poorly positively dependent on the nitrogen contained in the fuel. In addition, it has been shown that the fuel geometric features greatly influence NO content in the exhaust gases in the first combustion phase. It is also indicated that further research is required, considering the possibility of reducing volatile flue gas fractions, which will lead to the development of low-emission and highly efficient biofuel combustion technologies in low-power heating devices.
Optimization Trends in Demand-Side Management
Authors: Gutiérrez Álvaro
Published: August 2022

The electrical system is undergoing a structural change, dissolving into a new concept of a distributed environment, based on renewable energies, demand management and resource sharing [...]
Impact of Time Resolution on Curtailment Losses in Hybrid Wind-Solar PV Plants
Authors: Meglic Antun , Goic Ranko
Published: August 2022

Curtailment losses for large-scale hybrid wind–solar photovoltaic (PV) plants with a single grid connection point are often calculated in 1 h time resolution, underestimating the actual curtailment losses due to the flattening of power peaks occurring in shorter time frames. This paper analyses the curtailment losses in hybrid wind–PV plants by utilising different time resolutions of wind and PV production while varying the grid cut-off power, wind/solar PV farm sizes, and shares of wind/PV capacity. Highly resolved 1 s measurements from the operational wind farm and pyranometer are used as an input to specialized wind and PV farm power production models that consider the smoothing effect. The results show that 15 min resolution is preferred over 1 h resolution for large-scale hybrid wind–PV plants if more accurate assessment of curtailment losses is required. Although 1 min resolution additionally increases the estimation accuracy over 15 min resolution, the improvement is not significant for wind and PV plants with capacity above approx. 10 MW/10 MWp. The resolutions shorter than 1 min do not additionally increase the estimation accuracy for large-scale wind and PV plants. More attention is required when estimating curtailment losses in wind/PV plants with capacity below approx. 10 MW/10 MWp, where higher underestimation can be expected if lower time resolutions are used.
Developing a Yield Table and Analyzing the Economic Feasibility for Acacia Hybrid Plantations in Achieving Carbon Neutrality in Southern Vietnam
Authors: Lee Sang-Hyun , Kim Dong-Hyuk , Jeong Jin-Heon , Han Seung-Hyun , Kim Seongjun , Park Hee-Jung , Kim Hyun-Jun
Published: August 2022

To achieve the goal of carbon neutrality, overseas plantation projects have been conducted in several countries, including Vietnam. In the present study, stand growth models and a yield table were developed and used to analyze the economic feasibility of Acacia hybrid plantations in southern Vietnam. Diameter at breast height, tree height, and number of trees were determined in the field; basal area, mean height, dominant tree height, stand density, and stand volume were estimated using in situ data. The initial number of trees increased for five years and reached 4947 trees ha−1; tree numbers then decreased to 1987 trees ha−1 until the stands reached ten years of age. The highest current annual increment of stand volume was shown to be 46.23 m3 ha−1 yr−1 when stands were 7 years old. For 7 years of stand age, the net present value was USD 1566/ha, and the internal rate of return was 13%, exceeding the market interest rate (4%). Therefore, an A. hybrid plantation is a reasonable proposition for an overseas plantation project in southern Vietnam and the growth models will be useful for the management of an A. hybrid plantation.
Operating Characteristics of a Timber Trailer with a Hybrid Drive
Authors: Zemánek Tomáš , Procházka Petr , Pazdera Ivo , Neruda Jindřich , Mergl Václav , Vítek Ondřej , Ulrich Radomír , Staněk Luboš
Published: August 2022

This paper deals with the design and operational evaluation of a timber tractor-trailer unit with a hybrid trailer drive. The source of electrical energy for the two induction motors driving the front wheels of the tandem trailer axle is a battery, which is recharged by an induction machine operating as a generator during periods of a lower demand for power from the tractor diesel engine. An electric drive was designed for the defined working cycle of the tractor-trailer unit, and its loading characteristics were tested in the laboratory. The parameters measured on the field tests during timber forwarding were battery voltage and power, and the energy balance. Three adjustment levels of the potentiometer controlling the trailer hybrid drive (50, 75 and 100%) were tested at three different forwarding distances of 100, 500 and 1000 m. Additionally, any slippage of the prime mover wheels and trailer was measured. The maximum peak power taken from the battery was ca. 33 kW during the field tests, whilst the drive was able to deliver a peak output of up to 72 kW for 10 s and permanently up to ca. 50 kW. Even in harsh terrain conditions, the electric drive assisted the combustion engine only when the loaded tractor-trailer unit was travelling uphill. The hybrid drive operation was sustainable for the whole working shift, without the need for recharging when the potentiometer was set to 50%. This appropriate setting of the potentiometer controlling the trailer’s hybrid drive reduced the slipping of the driven wheels of the tractor-trailer unit whilst travelling uphill.
Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast
Authors: Moguel Ignacio S. , Yamakawa Celina K. , Brumano Larissa P. , Pessoa Adalberto , Mussatto Solange I.
Published: August 2022

This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for the treatment of acute lymphoblastic leukemia, by Leucosporidiumscottii L115, a psychrotolerant yeast isolated from the Antarctic ecosystem. Focus was given to select the most appropriate medium components able to maximize the enzyme production by this yeast, as a first step for the development of a new process to produce ASNase. By combining knowledge in bioprocesses, statistical analysis and modeling, the medium composition that most favored enzyme production was established, which consisted of using a mixture of sucrose (28.34 g L−1) and glycerol (15.61 g L−1) as carbon sources, supplemented with proline (6.15 g L−1) and the following salts (g L−1): KCl, 0.52; MgSO4·7H2O, 0.52; CuNO3·3H2O, 0.001; ZnSO4·7H2O, 0.001; and FeSO4·7H2O, 0.001. By using this medium, enzyme production of 2850 U L−1 (productivity of 23.75 U L−1 h−1) was obtained, which represented a 28-fold increase in enzyme production per gram of cells (178 U gdcw−1) when compared to the control (non-optimized medium), and a 50-fold increase when compared to a reference medium used for ASNase production.
On the Potential of Enhancing Delay-Tolerant Routing Protocols via Age of Information
Authors: Kallitsis Georgios , Karyotis Vasileios , Papavassiliou Symeon
Published: August 2022

In this paper, we study the potential of using the metric of Age of Information (AoI) for enhancing delay-tolerant routing protocols. The latter have been proposed for alleviating the impact of long roundtrip time in networks operating in harsh environments, e.g., in distributed applications deployed in a desert/sparsely populated area without infrastructure, a space network, etc. Delay-tolerant routing protocols can prevent excessive packet timer expiration, but do not provide any packet delivery time guarantee. Thus, they are unsuitable for time-sensitive applications that are more intensely desired nowadays in the next generation networking applications. By incorporating AoI into the operation of delay-tolerant routing protocols, we aim at devising routing protocols that can cope with both long propagation times and challenges related to time-sensitivity in packet delivery. More specifically, in this work, we modify the operation of a well-known delay-tolerant routing protocol, namely FRESH, to make AoI-based packet forwarding decisions, aiming at achieving specific delay guarantees regarding the end-to-end delivery time. We investigate the advantages and disadvantages of such an approach compared to the traditional FRESH protocol. This work serves as a cornerstone for successfully demonstrating the potential of exploiting AoI in delay-tolerant routing and its applications.
Dual Modification of Starch by Physical Methods Based on Corona Electrical Discharge and Ionizing Radiation: Synergistic Impact on Rheological Behavior
Authors: Braşoveanu Mirela , Nemţanu Monica R.
Published: August 2022

The present paper focuses on evaluating the synergistic effects of dual modification with corona electric discharge (CED) and electron beam irradiation (EBI) on the rheological behavior of starch. Combined treatments were applied successively (CED/EBI and EBI/CED) and compared with single treatments. The outcomes showed that the rheological features of starch were altered by the dual modification in correlation with the irradiation dose mainly as a result of radiation-induced degradation. Decreases in apparent viscosity were described by exponential-like-models according to the order of application of the treatment sequences. The mathematical models allowed the estimation of the irradiation doses for which the viscosity decreased by e times for the dual modified starches (3.3 ± 1.3 kGy for CED/EBI and 5.6 ± 0.5 kGy for EBI/CED, respectively) and the fraction (f) of 0.47 ± 0.10 corresponding to starch granule considered to be affected by plasma. Both dual treatments yielded a synergistic effect, regardless of the order of application of the treatment sequences, being more effective in decreasing starch apparent viscosity than single EBI. However, synergism evaluation proved that the use of plasma as a pre-treatment to irradiation processing could provide benefits up to 20 kGy. These findings support the practical goals of technologists with valuable information that may facilitate or simplify the experimental design of starch dual modification with plasma and ionizing radiation.
Dietary Supplementation with Sea Buckthorn Berry Puree Alters Plasma Metabolomic Profile and Gut Microbiota Composition in Hypercholesterolemia Population
Authors: Chen Kang , Zhou Fangfei , Zhang Jian , Li Pin , Zhang Yumei , Yang Baoru
Published: August 2022

Sea buckthorn berries have been reported to have beneficial effects on plasma lipid profile and cardiovascular health. This study aimed to investigate the impact of intervention with sea buckthorn berry puree on plasma metabolomics profile and gut microbiota in hypercholesterolemic subjects. A total of 56 subjects with hypercholesterolemia consumed 90 g of sea buckthorn berry puree daily for 90 days, and plasma metabolomic profile was studied at 0 (baseline), 45, and 90 days of intervention by using proton nuclear magnetic resonance spectroscopy (1H NMR). Gut microbiota composition was analyzed at the baseline and after 90 days of supplementation by using high-throughput sequencing. The plasma metabolic profile was significantly altered after 45 days of intervention as compared to the baseline (day 0). A clear trend of returning to the baseline metabolomic profile was observed in plasma when the intervention extended from 45 days to 90 days. Despite this, the levels of several key plasma metabolites such as glucose, lactate, and creatine were lowered at day 90 compared to the baseline levels, suggesting an improved energy metabolism in those patients. In addition, intervention with sea buckthorn puree enriched butyrate-producing bacteria and other gut microbes linked to lipid metabolisms such as Prevotella and Faecalibacterium while depleting Parasutterella associated with increased risks of cardiovascular disease. These findings indicate that sea buckthorn berries have potential in modulating energy metabolism and the gut microbiota composition in hypercholesterolemic patients.
Metataxonomic Mapping of the Microbial Diversity of Irish and Eastern Mediterranean Cheeses
Authors: Kamilari Eleni , Tsaltas Dimitrios , Stanton Catherine , Ross R. Paul
Published: August 2022

The distinct sensorial characteristics of local cheeses influence consumer preferences, and make an essential contribution to the local economy. Microbial diversity in cheese is among the fundamental contributors to sensorial and qualitative characteristics. However, knowledge regarding the existence of microbial patterns associated with regional production practices in ripened cheeses remains limited. The present research was conducted to test the hypothesis that the background metagenome of cheeses could be used as a marker of their origin. We compared Irish versus Eastern Mediterranean cheeses—namely Greek and Cypriot—using High Throughput Sequencing (HTS). The study identified a significantly distinct separation among cheeses originating from the three different countries, in terms of the total microbial community composition. The use of machine learning and biomarkers discovery algorithms defined key microbes that differentiate each geographic region. Finally, the development of interaction networks revealed that the key species developed mostly negative interactions with the other members of the communities, highlighting their dominance in the community. The findings of the present research demonstrate that metagenome could indeed be used as a biological marker of the origin of mature cheeses, and could provide further insight into the dynamics of microbial community composition in ripened cheeses.
Central Composite Design (CCD) for the Optimisation of Ethosomal Gel Formulation of Punica granatum Extract: In Vitro and In Vivo Evaluations
Authors: Alam Prawez , Shakeel Faiyaz , Foudah Ahmed I. , Alshehri Sultan , Salfi Roshan , Alqarni Mohammed H. , Aljarba Tariq M.
Published: August 2022

This research manuscript’s objective was to develop the Punica granatum extract ethosome gel. The use of nanotechnology can improve transdermal drug delivery permeation of its major bioactive compound β-sitosterol. The optimised and developed formulations were further studied in vitro and in vivo. The assessment of the anti-inflammatory activity of the gel was performed in Albino rats. Methanolic extract was prepared and developed into an ethosome suspension and an ethosome gel. To optimise the formulation’s response in terms of particle size (nm) and entrapment efficiency (%), the central composite design (CCD) was used in 22 levels. The effects of factors such as lecithin (%) and ethanol (mL) in nine formulations were observed. Characterisation of ethosome gel was performed and the results showed the particle size (516.4 nm) and mean zeta potential (−45.4 mV). Evaluations of the gel formulation were performed. The results were good in terms of pH (7.1), viscosity (32,158 cps), spreadability (31.55 g cm/s), and no grittiness. In an in vitro study, the percentages of β-sitosterol release of ethosome gel (91.83%), suspension (82.74%), and extracts (68.15%) at 279 nm were recorded. The effects of the formulated gel on formalin-induced oedema in Albino rats showed good results in terms of anti-inflammatory activity. The comparative anti-inflammatory activity of Punica granatum extract and gel showed that the gel action was good for their topical application.
Insulin Receptor Substrate 1 Gly972Arg (rs1801278) Polymorphism Is Associated with Obesity and Insulin Resistance in Kashmiri Women with Polycystic Ovary Syndrome
Authors: Rasool Shayaq Ul Abeer , Nabi Mudasar , Ashraf Sairish , Amin Shajrul
Published: August 2022

Background: Polycystic ovary syndrome (PCOS) is commonly associated with metabolic abnormalities such as hyperinsulinemia, insulin resistance and obesity. The genetic variants of genes regulating insulin action, expression and regulation are suggested as possible factors involved in development and severity of clinical manifestations in PCOS. Aim: We investigated whether IRS-1Gly972Arg (rs1801278) polymorphism is associated with increased risk of PCOS in Kashmiri women. The correlation of various clinical, metabolic and hormonal markers with rs1801278 single nucleotide polymorphism was analyzed. The genotypic–phenotypic association of clinical manifestations of PCOS with the tested genetic variant was also assessed. Results: There were no significant differences in allele frequency (OR = 0.87, CI = 0.59–1.29, χ2 = 0.456, p = 0.499) or genotypic distribution (χ2 = 3.73, p = 0.15) between PCOS women and controls. No significant association was also found in the dominant (OR = 1.63, χ2 = 0.377, p = 0.53), recessive (OR = 0.79, χ2 = 1.01, p = 0.31) or heterozygote vs. homozygote (OR = 1.34, χ2 = 1.53, p = 0.22) genotype model analysis. The genotype–phenotype correlation analysis showed that the Arg allele was significantly associated with increased central adiposity markers hip circumference (p = 0.012), and body adiposity index BAI (p = 0.002) in the recessive model in PCOS women. The two-hour glucose (p = 0.04) and insulin resistance marker HOMA (p = 0.44) were significantly higher in Arg allele carriers. The androgen excess markers dehydroepiandrosterone sulfate DHEAS (p = 0.02), Ferriman–Gallwey score (p = 0.012), prevalence of acne, alopecia and hirsutism (all p < 0.01) were significantly elevated in the wild-type GG genotype. Conclusions:IRS-1Gly972Arg genetic variant does not increase the risk of PCOS in Kashmiri women. However, this polymorphism is associated with clinical manifestations of insulin resistance, obesity and hyperandrogenism, suggesting its possible role in variable phenotypic manifestations of PCOS.
Analysis of Protein Sequence Identity, Binding Sites, and 3D Structures Identifies Eight Pollen Species and Ten Fruit Species with High Risk of Cross-Reactive Allergies
Authors: Zhou Wei , Bias Kaylah , Lenczewski-Jowers Dylan , Henderson Jiliah , Cupp Victor , Ananga Anthony , Ochieng Joel Winyo , Tsolova Violeta
Published: August 2022

Fruit allergens are proteins from fruits or pollen that cause allergy in humans, an increasing food safety concern worldwide. With the globalization of food trade and changing lifestyles and dietary habits, characterization and identification of these allergens are urgently needed to inform public awareness, diagnosis and treatment of allergies, drug design, as well as food standards and regulations. This study conducted a phylogenetic reconstruction and protein clustering among 60 fruit and pollen allergens from 19 species, and analyzed the clusters, in silico, for cross-reactivity (IgE), 3D protein structure prediction, transmembrane and signal peptides, and conserved domains and motifs. Herein, we wanted to predict the likelihood of their interaction with antibodies, as well as cross-reactivity between the many allergens derived from the same protein families, as the potential for cross-reactivity complicates the management of fruit allergies. Phylogenetic analysis classified the allergens into four clusters. The first cluster (n = 9) comprising pollen allergens showed a high risk of cross-reactivity between eight allergens, with Bet v1 conserved domain, but lacked a transmembrane helix and signal peptide. The second (n = 10) cluster similarly suggested a high risk of cross-reactivity among allergens, with Prolifin conserved domain. However, the group lacked a transmembrane helix and signal peptide. The third (n = 13) and fourth (n = 29) clusters comprised allergens with significant sequence diversity, predicted low risk of cross-reactivity, and showed both a transmembrane helix and signal peptide. These results are critical for treatment and drug design that mostly use transmembrane proteins as targets. The prediction of high risk of cross-reactivity indicates that it may be possible to design a generic drug that will be effective against the wide range of allergens. Therefore, in the past, we may have avoided the array of fruit species if one was allergic to any one member of the cluster.