bluuebunny/crossref_metadata_2025 huggingface dataset:
Design Matching and Dynamic Performance Test for an HST-Based Drive System of a Hillside Crawler Tractor
Authors: Liu Zhijie , Zhang Guoqiang , Chu Guoping , Niu Hanlin , Zhang Yazhou , Yang Fuzeng
Published: May 2021

In recent years, research into and development of hillside tractors has become a popular topic in the field of agricultural engineering in China. To solve the main problems associated with a low adjustment range of the working speed, complex operation, and low safety for slope operation of medium-sized crawler tractors, a hydrostatic drive system that can be used for hillside crawler tractors was designed. According to the operation requirements of a hillside crawler tractor, the parameters of the three-cylinder diesel engine, hydrostatic transmission (HST), drive rear axle, and other key components of the drive system were matched after the force and motion analyses of the tractor, and then the main performance indicators, including the traction performance, system pressure and working speed of the drive system were verified. On this basis, a drive system performance test bench was built, and the traction performance and starting acceleration performance of the drive system was tested. The results of the traction bench test show that when the engine was at the maximum torque point of 1700 r/min, the maximum theoretical tractive force outputted by the tractor in Gear I was 114,563 N, and the maximum theoretical tractive force outputted by tractor in Gear II was 10,959.2 N, which were both larger than the traction resistance of 9550.6 N experienced by the hillside tractor ploughing on the slope. The results of the initial acceleration bench test show that the tractor driving speed can gradually increase with increasing output of the variable pump and can reach the maximum in 3 s. When the tractor was driving on flat ground, the maximum driving speeds of Gear I, Gear II, and Gear III were 4.65 km/h, 6.58 km/h, and 8.57 km/h, respectively, which are close to the theoretical values. When the tractor was driving on a 15° slope, the maximum driving speeds of Gear I, Gear II, and Gear III were 4.55 km/h, 6.25 km/h, and 8.28 km/h, respectively. It can be concluded that the design matching of the drive system is reasonable, the speed consistency is good and there is enough power reserve, which can meet the requirements for a large workload.
Nocturnal Bees as Crop Pollinators
Authors: Cordeiro Guaraci D. , Liporoni Rodolfo , Caetano Carolina A. , Krug Cristiane , Martínez-Martínez Carlos A. , Martins Herbeson O. J. , Cardoso Renan K. O. A. , Araujo Fernanda F. , Araújo Priscila C. S. , Oliveira Reisla , Schlindwein Clemens , Warrant Eric J. , Dötterl Stefan , Alves-dos-Santos Isabel
Published: May 2021

Bees are typically diurnal but around 1% of described species have nocturnal activity. Nocturnal bees are still poorly studied due to bias towards studying diurnal insects. However, knowledge concerning their biology and role as crop pollinators has increased. We review the literature on nocturnal bees’ traits and their host plants, and assess the crop pollination effectiveness of this neglected group. Nocturnal bees have visual adaptations to cope with low light intensities, and floral scents are a key sensory cue used to find their host flowers. Nocturnal bees generally show high flower constancy, the ability to vibrate flowers, and high transfer rates of pollen grains to stigmas. The flowers visited by nocturnal bees range from small radial and zygomorphic flowers to large brush blossoms; moreover, they visit plants with different flowering strategies. Nocturnal bees are effective pollinators of regional fruit crops in Brazil, such as cambuci (Campomanesia phaea), guaraná (Paullinia cupana), cajá (Spondias mombin), and in North America of cultivated pumpkins (Cucurbita species). However, they most likely are pollinators of several other crops. Strategies to host high numbers of nocturnal bees around cropping areas should be taken, such as preserving adjacent native forests, restricting soil management, providing food resources beyond crop flowers, and avoiding light pollution.
Educational Video Improves Knowledge about Outpatients’ Usage of Antibiotics in Two Public Hospitals in Indonesia
Authors: Herawati Fauna , Yulia Rika , Arifin Bustanul , Frasetyo Ikhwan , Setiasih  , Woerdenbag Herman J. , Avanti Christina , Andrajati Retnosari
Published: May 2021

The inappropriate use or misuse of antibiotics, particularly by outpatients, increases antibiotic resistance. A lack of public knowledge about “Responsible use of antibiotics” and “How to obtain antibiotics” is a major cause of this. This study aimed to assess the effectiveness of an educational video about antibiotics and antibiotic use to increase outpatients’ knowledge shown in two public hospitals in East Java, Indonesia. A quasi-experimental research setting was used with a one-group pre-test—post-test design, carried out from November 2018 to January 2019. The study population consisted of outpatients to whom antibiotics were prescribed. Participants were selected using a purposive sampling technique; 98 outpatients at MZ General Hospital in the S regency and 96 at SG General Hospital in the L regency were included. A questionnaire was used to measure the respondents’ knowledge, and consisted of five domains, i.e., the definition of infections and antibiotics, obtaining the antibiotics, directions for use, storage instructions, and antibiotic resistance. The knowledge test score was the total score of the Guttman scale (a dichotomous “yes” or “no” answer). To determine the significance of the difference in knowledge before and after providing the educational video and in the knowledge score between hospitals, the (paired) Student’s t-test was applied. The educational videos significantly improved outpatients’ knowledge, which increased by 41% in MZ General Hospital, and by 42% in SG General Hospital. It was concluded that an educational video provides a useful method to improve the knowledge of the outpatients regarding antibiotics.
Sensitivity of Staphylococcal Biofilm to Selected Compounds of Plant Origin
Authors: Swolana Denis , Kępa Małgorzata , Kabała-Dzik Agata , Dzik Radosław , Wojtyczka Robert D.
Published: May 2021

Staphylococcus epidermidis is a bacterium that belongs to the human microbiota. It is most plentiful on the skin, in the respiratory system, and in the human digestive tract. Moreover, it is the most frequently isolated microorganism belonging to the group of Coagulase Negative Staphylococci (CoNS). In recent years, it has been recognized as an important etiological factor of mainly nosocomial infections and infections related to the cardiovascular system. On the other hand, Staphylococcus aureus, responsible for in-hospital and out-of-hospital infections, is posing an increasing problem for clinicians due to its growing resistance to antibiotics. Biofilm produced by both of these staphylococcal species in the course of infection significantly impedes therapy. The ability to produce biofilm hinders the activity of chemotherapeutic agents—the only currently available antimicrobial therapy. This also causes the observed significant increase in bacterial resistance. For this reason, we are constantly looking for new substances that can neutralize microbial cells. In the present review, 58 substances of plant origin with antimicrobial activity against staphylococcal biofilm were replaced. Variable antimicrobial efficacy of the substances was demonstrated, depending on the age of the biofilm. An increase in the activity of the compounds occurred in proportion to increasing their concentration. Appropriate use of the potential of plant-derived compounds as an alternative to antibiotics may represent an important direction of change in the support of antimicrobial therapy.
Solid-State Lithium Battery Cycle Life Prediction Using Machine Learning
Authors: Cheng Danpeng , Sha Wuxin , Wang Linna , Tang Shun , Ma Aijun , Chen Yongwei , Wang Huawei , Lou Ping , Lu Songfeng , Cao Yuan-Cheng
Published: May 2021

Battery lifetime prediction is a promising direction for the development of next-generation smart energy storage systems. However, complicated degradation mechanisms, different assembly processes, and various operation conditions of the batteries bring tremendous challenges to battery life prediction. In this work, charge/discharge data of 12 solid-state lithium polymer batteries were collected with cycle lives ranging from 71 to 213 cycles. The remaining useful life of these batteries was predicted by using a machine learning algorithm, called symbolic regression. After populations of breed, mutation, and evolution training, the test accuracy of the quantitative prediction of cycle life reached 87.9%. This study shows the great prospect of a data-driven machine learning algorithm in the prediction of solid-state battery lifetimes, and it provides a new approach for the batch classification, echelon utilization, and recycling of batteries.
Validation of a TAM Extension in Agriculture: Exploring the Determinants of Acceptance of an e-Learning Platform
Authors: Castiblanco Jimenez Ivonne Angelica , Cepeda García Laura Cristina , Marcolin Federica , Violante Maria Grazia , Vezzetti Enrico
Published: May 2021

Supporting education and training initiatives has been identified as an effective way to address Sustainable Development Challenges. In this sense, e-learning stands out as one of the most viable alternatives considering its advantages in terms of resources, time management, and geographical location. Understanding the reasons that move users to adopt these technologies is critical for achieving the desired social objectives. The Technology Acceptance Model (TAM) provides valuable guidelines to identify the variables shaping users’ acceptance of innovations. The present study aims to validate a TAM extension designed for FARMER 4.0, an e-learning application in the agricultural sector. Findings suggest that content quality (CQ) is the primary determinant of farmers’ and agricultural entrepreneurs’ perception of the tool’s usefulness (PU). Furthermore, experience (EXP) and self-efficacy (SE) shape potential users’ perceptions about ease of use (PEOU). This study offers helpful insight into the design and development of e-learning applications in the farming sector and provides empirical evidence of TAM’s validity to assess technology acceptance.
Antibacterial Activity of Moroccan Zantaz Honey and the Influence of Its Physicochemical Parameters Using Chemometric Tools
Authors: Elamine Youssef , Imtara Hamada , Miguel Maria Graça , Anjos Ofélia , Estevinho Letícia M. , Alaiz Manuel , Girón-Calle Julio , Vioque Javier , Martín Jesús , Lyoussi Badiâa
Published: May 2021

The emergence of multidrug-resistant bacteria has prompted the development of alternative therapies, including the use of natural products with antibacterial properties. The antibacterial properties of Zantaz honey produced in the Moroccan Atlas Mountains against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was evaluated and analyzed using chemometric tools. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) against S. aureus were the lowest (112.5 ± 54.5 mg/mL), revealing that this species was most sensitive to Zantaz honey. P. aeruginosa showed an intermediate sensitivity (MIC= 118.75 ± 51.9 mg/mL), while E. coli was the most resistant to treatment (MIC = 175 ± 61.2 mg/mL). Content of monosaccharides, certain minerals, and phenolic compounds correlated with antibacterial activity (p < 0.05). Principal component analysis of physicochemical characteristics and antibacterial activity indicated that the parameters most associated with antibacterial activity were color, acidity, and content of melanoidins, fructose, epicatechin, methyl syringate, 4-coumaric acid, and 3-coumaric acid.
Phase Change Material Integration in Building Envelopes in Different Building Types and Climates: Modeling the Benefits of Active and Passive Strategies
Authors: Carlucci Francesco , Cannavale Alessandro , Triggiano Angela Alessia , Squicciarini Amalia , Fiorito Francesco
Published: May 2021

Among the adaptive solutions, phase change material (PCM) technology is one of the most developed, thanks to its capability to mitigate the effects of air temperature fluctuations using thermal energy storage (TES). PCMs belong to the category of passive systems that operate on heat modulation, thanks to latent heat storage (LHS) that can lead to a reduction of heating ventilation air conditioning (HVAC) consumption in traditional buildings and to an improvement of indoor thermal comfort in buildings devoid of HVAC systems. The aim of this work is to numerically analyze and compare the benefits of the implementation of PCMs on the building envelope in both active and passive strategies. To generalize the results, two different EnergyPlus calibrated reference models—the small office and the midrise apartment—were considered, and 25 different European cities in different climatic zones were selected. For these analyses, a PCM plasterboard with a 23 °C melting point was considered in four different thicknesses—12.5, 25, 37.5, and 50 mm. The results obtained highlighted a strong logarithmic correlation between PCM thickness and energy reduction in all the climatic zones, with higher benefits in office buildings and in warmer climates for both strategies.
A Novel Maneuver-Based Driving Envelope Generation Approach for Driving Safety Assessment
Authors: Jiang Bohan , Li Xiaohui , Zeng Yujun , Liu Daxue
Published: May 2021

It is of utmost importance for advanced driver assistance systems to evaluate the risk of the current situation and make continuous decisions about what kind of evasive maneuver can be initiated. The purpose of this paper is to establish efficient indicators to evaluate the risk of candidate driving maneuvers for a human-in-the-loop vehicle. A novel safe driving envelope generation method is proposed, which takes various constraints into consideration, including the human operation, vehicle motion limits, and collision avoidance with road boundary and obstacles. The efficiency of the proposed method is validated by simulation experiments and real vehicle tests. The results show that the feasibility of candidate driving maneuvers can be efficiently determined by computing the driving envelope, and the proposed driving envelope method can be easily implemented for real-time applications.
Improvement of a Stitching Operation in the Stitching Linear-Scan Method for Measurement of Cylinders in a Small Dimension
Authors: Li Qiaolin , Shimizu Yuki , Saito Toshiki , Matsukuma Hiraku , Cai Yindi , Gao Wei
Published: May 2021

Attempts are made in this paper to improve the quality of the stitching between adjacent arc-profiles in the stitching linear-scan method for the roundness measurement of a cylinder in a small dimension. The data in the edge region of an arc-profile, which could be influenced by the pressure angle of the measurement probe of a linear-scan stylus profiler, are eliminated in the stitching process to improve the quality of stitching. The effectiveness of the elimination of the edge region of an arc-profile is evaluated by employing the cross-correlation coefficient of two adjacent arc-profiles as an evaluation index. Furthermore, a modification is made to the experimental setup to reduce the misalignment of a workpiece along its axial direction with respect to the scanning probe. Experiments are carried out by using the modified setup to demonstrate the feasibility of the stitching linear-scan method for the roundness measurement of a small cylinder, which is difficult to measure by the conventional rotary-scan method.
On the Estimation of the Moving Mass of a TMD Installed on a Lively Structure
Authors: Magdaleno Alvaro , Pelaez Cesar , Iglesias-Pordomingo Alvaro , Lorenzana Antolin
Published: May 2021

Tuned Mass Dampers are devices which can be assimilated to single-degree-of-freedom systems with a certain amount of moving mass, a natural frequency and a damping ratio intended to be installed on lively structures to reduce the contribution of a certain mode to their response. Once placed on the structure, the movement of the mass damper couples to the structural response and determines its properties as an isolated system becomes challenging. The authors have previously presented a methodology to estimate the natural frequency and damping ratio of an SDOF system installed on a structure and not necessarily tuned to a certain mode. It was based on a transmissibility function and, thus, the moving mass could not be estimated. With this work, the authors go one step further and present a novel procedure to estimate the moving mass value by means of the same transmissibility function and two well selected frequency response functions. The methodology is applied to estimate the properties of a real single-degree-of-freedom system placed on a lively timber platform. The results are compared with the mass modification technique to show that the proposed methodology provides better estimations in a more efficient way.
Transient Receptor Potential (TRP) Channels in Haematological Malignancies: An Update
Authors: Maggi Federica , Morelli Maria Beatrice , Nabissi Massimo , Marinelli Oliviero , Zeppa Laura , Aguzzi Cristina , Santoni Giorgio , Amantini Consuelo
Published: May 2021

Transient receptor potential (TRP) channels are improving their importance in different cancers, becoming suitable as promising candidates for precision medicine. Their important contribution in calcium trafficking inside and outside cells is coming to light from many papers published so far. Encouraging results on the correlation between TRP and overall survival (OS) and progression-free survival (PFS) in cancer patients are available, and there are as many promising data from in vitro studies. For what concerns haematological malignancy, the role of TRPs is still not elucidated, and data regarding TRP channel expression have demonstrated great variability throughout blood cancer so far. Thus, the aim of this review is to highlight the most recent findings on TRP channels in leukaemia and lymphoma, demonstrating their important contribution in the perspective of personalised therapies.
Survival Rates of Patients with Non-Small Cell Lung Cancer Depending on Lymph Node Metastasis: A Focus on Saliva
Authors: Bel’skaya Lyudmila V. , Sarf Elena A. , Kosenok Victor K.
Published: May 2021

The aim of this study was to compare overall survival (OS) rates at different pN stages of NSCLC depending on tumor characteristics and to assess the applicability of saliva biochemical markers as prognostic signs. The study included 239 patients with NSCLC (pN0-120, pN1-51, pN2-68). Saliva was analyzed for 34 biochemical indicators before the start of treatment. For pN0, the tumor size does not have a prognostic effect, but the histological type should be taken into account. For pN1 and pN2, long-term results are significantly worse in squamous cell cancer with a large tumor size. A larger volume of surgical treatment reduces the differences between OS. The statistically significant factors of an unfavorable prognosis at pN0 are the lactate dehydrogenase activity <1294 U/L and the level of diene conjugates >3.97 c.u. (HR = 3.48, 95% CI 1.21–9.85, p = 0.01541); at pN1, the content of imidazole compounds >0.296 mmol/L (HR = 6.75, 95% CI 1.28–34.57, p = 0.00822); at pN2 levels of protein <0.583 g/L and Schiff bases >0.602 c.u., as well as protein >0.583 g/L and Schiff bases <0.602 c.u. (HR = 2.07, 95% CI 1.47–8.93, p = 0.04351). Using salivary biochemical indicators, it is possible to carry out stratification into prognostic groups depending on the lymph node metastasis.
Transfer of the German Vocational Education and Training System—Success Factors and Hindrances with the Example of Tunisia
Authors: Oeben Melanie , Klumpp Matthias
Published: May 2021

As the German vocational education and training (VET) system, with its dual learning environment in both firms and vocational schools, is an international benchmark, many concepts and projects are aiming to transfer this approach to other countries and education systems. In such contexts, it is important to consider the specific concepts and requirements of educational transfer. This paper outlines success factors and hindrances for such endeavors. Therefore, a literature review is combined with empirical survey results from Germany. This is directed at a specific project regarding a transfer of the dual VET system from Germany to Tunisia, but it also exemplifies general factors for any such concept transfer. As important success factors, “quality assurance” and “perspectives for graduates”, for example, are identified.
Integral Sliding Control Approach for Generalized Cyclic Pursuit Formation Maintenance
Authors: Ansart Antoine , Juang Jyh-Ching
Published: May 2021

This paper is concerned with the formation maintenance of a group of autonomous agents under generalized cyclic pursuit (GCP) law. The described pattern for agents under such formation is epicycle-like. For a network of agents to achieve such a formation, marginal stability of the overall network is required. The desired marginal stability of the network relies on each agents’ gain values, and uncertainties in these gains can occur. Previous studies have used fixed gains, we enhance the stability of the gains via a dynamic approach using an integral sliding controller (ISC). An ISC can ensure sliding behavior of the gains throughout the entire response, and it is shown that the gains are robust toward variations and thus make the network keep its marginal stability and its formation.
Relationships among Consumer Liking, Lipid and Volatile Compounds from New Zealand Commercial Lamb Loins
Authors: Pavan Enrique , Ye Yangfan , Eyres Graham T. , Guerrero Luis , G. Reis Mariza , Silcock Patrick , Johnson Patricia L. , Realini Carolina E.
Published: May 2021

Loin sections (m. Longissimus lumborum) were collected at slaughter from forty-eight lamb carcasses to evaluate consumer-liking scores of six types of typical New Zealand commercial lamb and to understand the possible underlying reasons for those ratings. A consumer panel (n = 160) evaluated tenderness, juiciness, flavor liking, and overall liking of the different types of lamb loins. Consumer scores differed among the types of lamb meat for all the evaluated attributes (p < 0.05). Further segmentation based on overall liking scores showed two consumer clusters with distinct ratings. Correlation and external preference map analyses indicated that one consumer cluster (n = 75) liked lamb types that had lower total lipid content, a lower proportion of branched-chain fatty acids, oleic and heptadecanoic acids; and a higher proportion of polyunsaturated fatty acids and volatile compounds (green and fruity descriptors). Consumer liking of the other segment (n = 85) was less influenced by fatty acids and volatiles, except hexanoic, heptanoic and octanoic acids (rancid, fatty, and sweaty descriptors). Thus, the fatty acid profile and the volatile compounds derived from their oxidation upon cooking seem to be a stronger driver of consumer liking of lamb for some consumers than others.
Pulsed Electric Field (PEF) Processing of Chilled and Frozen-Thawed Lamb Meat Cuts: Relationships between Sensory Characteristics and Chemical Composition of Meat
Authors: Kantono Kevin , Hamid Nazimah , Chadha Diksha , Ma Qianli , Oey Indrawati , Farouk Mustafa M.
Published: May 2021

The effect of PEF processing and chilled storage on the volatile composition and sensory properties of chilled and frozen lamb cuts was investigated in this study. Results showed that PEF-treated chilled and frozen lamb cuts varied in temporal flavour attributes with storage. Storage for 7 days resulted in oxidized flavour, while PEF treatments for all chilled and frozen cuts were associated with browned and livery flavour attributes. Partial least squares regression (PLSR) was applied to determine the predictive relationships between the volatile composition, fatty acid and amino acid profiles, and sensory responses for PEF treated lamb cuts. The results showed that some volatile compounds (2-nonanone, 2-pentylfuran, pyrrole, methyl pyrazine, 2-ethyl-3-methyl pyrazine, and thiophene) correlated well with the meaty and juicy flavour of PEF treated frozen lamb cuts. In PEF treated chilled lamb cuts, meaty and juicy flavours were associated with the presence of fatty acids (C18:0, SFA, 20:5(n-3), and n-3). In contrast, livery and browned perception of both PEF processed chilled and frozen lamb cuts were associated with the presence of amino acids (threonine, phenylalanine, isoleucine, tyrosine, and methionine), and some volatile compounds (heptanal, 2-ethylfuran, pyridine, dimethyl disulphide, dimethyl trisulphide, and 3,5-diethyl-2-methyl pyrazine). Overall, these results imply that careful consideration of type of meat cuts, PEF pre-treatment, and storage are important when subjecting lamb meat to PEF processing.
Analysis of Built-Up Areas of Small Polish Cities with the Use of Deep Learning and Geographically Weighted Regression
Authors: Adamiak Maciej , Jażdżewska Iwona , Nalej Marta
Published: May 2021

Small cities are an important part of the settlement system, a link between rural areas and large cities. Although they perform important functions, research focuses on large cities and metropolises while marginalizing small cities, the study of which is of great importance to progress in social sciences, geography, and urban planning. The main goal of this paper was to verify the impact of selected socio-economic factors on the share of built-up areas in 665 small Polish cities in 2019. Data from the Database of Topographic Objects (BDOT), Sentinel-2 satellite imagery from 2015 and 2019, and Local Data Bank by Statistics Poland form 2019 were used in the research. A machine learning segmentation procedure was used to obtain the data on the occurrence of built-up areas. Hot Spot (Getis-Ord Gi*) analysis and geographically weighted regression (GWR) was applied to explain spatially varying impact of factors related to population, spatial and economic development, and living standards on the share of built-up areas in the area of small cities. Significant association was found between the population density and the share of built-up areas in the area of the cities studied. The influence of the other socio-economic factors examined, related to the spatial and economic development of the cities and the quality of life of the inhabitants, showed great regional variation. The results also indicated that the share of built-up areas in the area of the cities under study is a result of the conditions under which they were established and developed throughout their existence, and not only of the socio-economic factors affecting them at present.
Material Overconsumption as Ecological Polemics in Allen Ginsberg’s “Plutonian Ode” and Gary Snyder’s “Smokey the Bear Sutra”: Re-Envisioning Beat Critiques of Anthropocentric Materialism
Authors: Yulianto Henrikus Joko
Published: May 2021

Beat poetry, since its origination in the American milieu in the 1950s until its further maturation in the late 1960s and 1970s, has embodied ecological visions. Allen Ginsberg’s and Gary Snyder’s Buddhist poetics of the emptiness of material phenomena evoke one’s awareness ofthe true nature of material goods. This ecological awarenessenlightensanyoneto not overconsume the goods in fulfilling his/her daily necessities. In this recent era, Ginsberg’s “Plutonian Ode” and Snyder’s “Smokey the Bear Sutra” memorialize this Beat green poetics against anthropocentric materialism and its potential detrimental impacts on the natural environment. These poems view human’s material attachment as a recurring melancholia even in today’s digital technology era. Their ecological criticisms through the Buddhist poetics pave the way for anyone to cherish rather than objectify any material thing in living the biotic community.
Legal yet Illegitimate Governance in Italy
Authors: Pardo Italo
Published: May 2021

For several decades Naples has exemplified the deficit of legitimacy that now outstandingly mars public life in Italy and across many democracies. Drawing on ethnographic evidence from extended anthropological field research, this article examines the increasing gap between rulers and the ruled, which jeopardizes the authority, therefore legitimacy, of governance. With a focus on this major Italian city, the discussion leads to the conclusion that this gap and its ramifications are a substantial threat to democracy that urgently needs to be understood in depth and comprehensively, eschewing both conceptual superimposition and ideological bias organic to vested interests.
The Interactions between COVID-19 Cases in the USA, the VIX Index and Major Stock Markets
Authors: Grima Simon , Özdemir Letife , Özen Ercan , Romānova Inna
Published: May 2021

With this study, we aimed to determine (1) the effect of the daily new cases and deaths due to the COVID-19 pandemic in the United States on the CBOE volatility index (VIX index) and (2) the effect of the VIX index on the major stock markets during the early stage of the pandemic period. To do this, we collected and analysed the daily new cases and death numbers during the COVID-19 pandemic period in the United States and the country indexes of the USA (DJI), Germany (DAX), France (CAC40), England (FTSE100), Italy (MIB), China (SSEC) and Japan (Nikkei225) to determine the impact of the VIX index on the major stock markets. We then subjected this data to the Johansen co-integration test and the fully modified least-squares (FMOLS) method. The results indicated that there was co-integration between the VIX and the COVID-19 pandemic and that there was co-integration between the VIX index and major indexes, except for the CAC 40 and MIB. Moreover, the results showed that the new COVID-19 cases in the USA had a higher impact on the VIX than cases of deaths during the same period.
Automatic Delineation of Urban Growth Boundaries Based on Topographic Data Using Germany as a Case Study
Authors: Harig Oliver , Hecht Robert , Burghardt Dirk , Meinel Gotthard
Published: May 2021

Urban Growth Boundary (UGB) is a growth management policy that designates specific areas where growth should be concentrated in order to avoid urban sprawl. The objective of such a boundary is to protect agricultural land, open spaces and the natural environment, as well as to use existing infrastructure and public services more efficiently. Due to the inherent heterogeneity and complexity of settlements, UGBs in Germany are currently created manually by experts. Therefore, every dataset is linked to a specific area, investigation period and dedicated use. Clearly, up-to-date, homogeneous, meaningful and cost-efficient delineations created automatically are needed to avoid this reliance on manually or semi-automatically generated delineations. Here, we present an aggregative method to produce UGBs using building footprints and generally available topographic data as inputs. It was applied to study areas in Frankfurt/Main, the Hanover region and rural Brandenburg while taking full account of Germany’s planning and legal framework for spatial development. Our method is able to compensate for most of the weaknesses of available UGB data and to significantly raise the accuracy of UGBs in Germany. Therefore, it represents a valuable tool for generating basic data for future studies. Application elsewhere is also conceivable by regionalising the employed parameters.
Clinical Manifestations of COVID-19 in the Feet: A Review of Reviews
Authors: Jimenez-Cebrian Ana Maria , Castro-Mendez Aurora , García-Podadera Blanca , Romero-Galisteo Rita , Medina-Alcántara Miguel , Garcia-Paya Irene , Páez-Moguer Joaquín , Córdoba-Fernández Antonio
Published: May 2021

There is a lack of scientific evidence about the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The clinical manifestations are not thoroughly understood; classically, the virus manifests itself at the pulmonary level but can manifest at other levels. To the best of our knowledge, systematic reviews and non-systematic reviews about COVID-19 symptoms in the feet have not been published. The aim of this review of reviews was to analyze and synthesize the published reviews on manifestations of COVID-19 at the foot level. Methods: a review of reviews was conducted; the eligibility criteria included studies published in English or Spanish, involving children and adults with COVID-19, and reporting foot manifestations. PubMed, SciELO, Science Direct, Cochrane Database of Systematic Review, and Google Scholar were analyzed. Two authors independently performed the screening and quality assessment of the studies with AMSTAR 1, and finally, nine reviews were analyzed (one systematic and eight narratives studies). The main clinical manifestations at the foot level in patients with COVID-19 were vascular (edema, exanthems, chilblains, ischemia, and distal necrosis), dermatological (vesicular, maculopapular, papulosquamous, urticarial skin breakouts, and recurrent herpes), and neurological (muscular weakness in lower limbs, paresis, areflexias, ataxia, and difficulty walking). Erythema pernio or “COVID toes” was shown as the most characteristic lesion of this disease, especially in asymptomatic children and young people, so this typical manifestation may be considered important in patients who are positive for COVID-19. This finding does not allow for strong conclusions due to the scarce literature and methodological quality in this regard. Future studies are necessary.
Biomarkers to Guide the Timing of Surgery: Neutrophil and Monocyte L-Selectin Predict Postoperative Sepsis in Orthopaedic Trauma Patients
Authors: Briggs Gabrielle Daisy , Lemmert Karla , Lott Natalie Jane , de Malmanche Theo , Balogh Zsolt Janos
Published: May 2021

Deciding whether to delay non-lifesaving orthopaedic trauma surgery to prevent multiple organ failure (MOF) or sepsis is frequently disputed and largely based on expert opinion. We hypothesise that neutrophils and monocytes differentially express activation markers prior to patients developing these complications. Peripheral blood from 20 healthy controls and 162 patients requiring major orthopaedic intervention was collected perioperatively. Neutrophil and monocyte L-selectin, CD64, CD11, CD18, and CXCR1 expression were measured using flow cytometry. The predictive ability for MOF and sepsis was assessed using the Receiver Operating Characteristic (ROC) comparing to C-reactive protein (CRP). Neutrophil and monocyte L-selectin were significantly higher in patients who developed sepsis. Neutrophil L-selectin (AUC 0.692 [95%CI 0.574–0.810]) and monocyte L-selectin (AUC 0.761 [95%CI 0.632–0.891]) were significant predictors of sepsis and were not significantly different to CRP (AUC 0.772 [95%CI 0.650–0.853]). Monocyte L-selectin was predictive of MOF preoperatively and postoperatively (preop AUC 0.790 [95%CI 0.622–0.958]). CD64 and CRP were predictive of MOF at one-day postop (AUC 0.808 [95%CI 0.643–0.974] and AUC 0.809 [95%CI 0.662–0.956], respectively). In the perioperative period, elevated neutrophil and monocyte L-selectin are predictors of postoperative sepsis. Larger validation studies should focus on these biomarkers for deciding the timing of long bone/pelvic fracture fixation.
Arrhythmogenic Left Ventricular Cardiomyopathy: Genotype-Phenotype Correlations and New Diagnostic Criteria
Authors: Mattesi Giulia , Cipriani Alberto , Bauce Barbara , Rigato Ilaria , Zorzi Alessandro , Corrado Domenico
Published: May 2021

Arrhythmogenic cardiomyopathy (ACM) is an inherited heart muscle disease characterized by loss of ventricular myocardium and fibrofatty replacement, which predisposes to scar-related ventricular arrhythmias and sudden cardiac death, particularly in the young and athletes. Although in its original description the disease was characterized by an exclusive or at least predominant right ventricle (RV) involvement, it has been demonstrated that the fibrofatty scar can also localize in the left ventricle (LV), with the LV lesion that can equalize or even overcome that of the RV. While the right-dominant form is typically associated with mutations in genes encoding for desmosomal proteins, other (non-desmosomal) mutations have been showed to cause the biventricular and left-dominant variants. This has led to a critical evaluation of the 2010 International Task Force criteria, which exclusively addressed the right phenotypic manifestations of ACM. An International Expert consensus document has been recently developed to provide upgraded criteria (“the Padua Criteria”) for the diagnosis of the whole spectrum of ACM phenotypes, particularly left-dominant forms, highlighting the use of cardiac magnetic resonance. This review aims to offer an overview of the current knowledge on the genetic basis, the phenotypic expressions, and the diagnosis of left-sided variants, both biventricular and left-dominant, of ACM.
Development of Metabolic Syndrome Decreases Bone Mineral Density T-Score of Calcaneus in Foot in a Large Taiwanese Population Follow-Up Study
Authors: Chiu Hsuan , Lee Mei-Yueh , Wu Pei-Yu , Huang Jiun-Chi , Chen Szu-Chia
Published: May 2021

Studies have suggested that there may be common pathogenic pathways linking osteoporosis and metabolic syndrome (MetS) due to the multiple risk factors for atherosclerotic cardiovascular disease caused by MetS. However, results on the association between MetS and bone health are inconsistent and sometimes contradictory. In this study, we aimed to investigate the associations between the effects of MetS risk factors and bone mineral density (BMD) T-score in a longitudinal study of 27,033 participants from the Taiwan Biobank with a follow-up period of 4 years. BMD of the calcaneus was measured in the non-dominant foot using ultrasound in the Taiwanese population. The overall prevalence rates of MetS were 16.7% (baseline) and 21.2% (follow-up). The participants were stratified into four groups according to the status of MetS (no/yes at baseline and follow-up). We investigated associations between MetS and its five components (baseline, follow-up) with BMD ΔT-score and found that the (no, yes) MetS group, (no, yes) abdominal obesity group, (no, yes) hypertriglyceridemia group, and (no, yes) low high-density lipoprotein (HDL) cholesterol group had the lowest ΔT-score. Furthermore, in the (no, yes) MetS group, high Δwaist circumference (p = 0.009), high Δtriglycerides (p = 0.004), low ΔHDL cholesterol (p = 0.034), and low Δsystolic blood pressure (p = 0.020) were significantly associated with low ΔT-score, but Δfasting glucose was not. In conclusion, in this large population-based cohort study, our data provide evidence that the development of MetS is strongly associated with increased rates of BMD loss in the Taiwanese population. This suggests that the prevention of MetS should be taken into consideration in the prevention of osteoporosis in the Taiwanese population.
Iguratimod: Novel Molecular Insights and a New csDMARD for Rheumatoid Arthritis, from Japan to the World
Authors: Nozaki Yuji
Published: May 2021

Iguratimod (IGU) is a conventional synthetic disease-modifying anti-rheumatic drug (csDMARD) routinely prescribed in Japan since 2012 to patients with rheumatoid arthritis (RA). Iguratimod acts directly on B cells by inhibiting the production of inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-8, IL-17), thereby suppressing the production of immunoglobulin and inhibiting the activity of nuclear factor kappa-light chain enhancer of activated B cells. In Japan, it is one of the most used csDMARDs in daily practice, but it is not recommended as a treatment for RA due to the lack of large-scale evidence established overseas. However, recent reports on the novel pharmacological effects of IGU on lymphocytes and synovial fibroblasts, as well as its efficacy in daily practice, have increased its importance as a drug for the treatment of RA. In this review, we highlighted the basic and clinical studies in IGU and discuss its potential as a new therapeutic agent for the treatment of RA.
Comparing Endovascular Treatment Methods in Acute Ischemic Stroke Due to Tandem Occlusion Focusing on Clinical Aspects
Authors: Kalmar Peter Janos , Tarkanyi Gabor , Nagy Csaba Balazs , Csecsei Peter , Lenzser Gabor , Bosnyak Edit , Karadi Zsofia Nozomi , Annus Adam , Szegedi Istvan , Buki Andras , Szapary Laszlo
Published: May 2021

Introduction: Acute ischemic strokes (AIS) due to tandem occlusion (TO) of intracranial anterior large vessel and concomitant extracranial internal carotid artery (EICA) are represent in 15–20% of all ischemic strokes. The endovascular treatment (EVT) strategy for those patients is still unclear. Although the intracranial mechanical thrombectomy (MT) is considered as a standard treatment approach, the EICA lesion stent necessity remains a matter of debate. We sought to assess the efficacy and safety of EVT in tandem lesions, particularly the EICA stenting management. Methods: We retrospectively analyzed all patients with anterior circulation stroke associated with EICA lesion and receiving EVT in the three participated stroke centers between November 2017 and December 2020. Patients’ data were collected from our prospective stroke registry (STAY ALIVE). Patients enrolled in our study were divided into two groups depending on whether acute carotid stenting (ACS) or balloon angioplasty only (BAO) technique was used. Our primary outcome was the 90-day functional outcome assessed by modified Rankin scale (mRS). Mortality at 90 days and symptomatic intracranial hemorrhage (sICH) were considered as secondary outcomes. Results: A total of 101 patients (age: 67 ± 10 years, 38.6% female) were enrolled in our study, including 29 (28.3%) BAO cases, and 72 (71.3%) patients treated with ACS. Patients in the BAO group were slightly older (70 ± 9 years vs. 66 ± 10 years, p = 0.054), and had higher prevalence of comorbidities such as hypertension (100.0% vs. 59.4%, p < 0.001). There was no significant difference in favorable outcomes (51.7% vs. 54.4%, p = 0.808) between the groups. However, we observed a trend towards higher rates of sICH (8.3% vs. 3.4%, p = 0.382) and 90-day mortality (23.5% vs. 13.8%, p = 0.278) with significantly higher frequency of distal embolization (39.1% vs. 17.9%, p = 0.043) in patients with ACS. In the overall population age (p = 0.013), atrial fibrillation (AF) (p = 0.008), National Institutes of Health Stroke Scale (NIHSS) baseline (p = 0.029), and successful recanalization (p = 0.023) were associated with favorable outcome. Conclusion: Endovascular approach of EICA in addition to MT was safe and effective in tandem occlusion of anterior circulation. Furthermore, our results suggest that balloon angioplasty technique without acute stenting shows a comparable favorable outcome rate to ACS with moderately less hemorrhagic events and mortality rates.
Contrasting Roles of Ang II and ACEA in the Regulation of IL10 and IL1β Gene Expression in Primary SHR Astroglial Cultures
Authors: Haspula Dhanush , Clark Michelle A.
Published: May 2021

Angiotensin (Ang) II is well-known to have potent pro-oxidant and pro-inflammatory effects in the brain. Extensive crosstalk between the primary Ang II receptor, Ang type 1 receptor (AT1R), and the cannabinoid type 1 receptor (CB1R) has been demonstrated by various groups in the last decade. Since activation of glial CB1R has been demonstrated to play a key role in the resolution of inflammatory states, we investigated the role of Ang II (100 nM) and/or ACEA (10 nM), a potent CB1R-specific agonist in the regulation of inflammatory markers in astrocytes from spontaneously hypertensive rats (SHR) and Wistar rats. Astrocytes were cultured from brainstems and cerebellums of SHR and Wistar rats and assayed for IL1β and IL10 gene expression and secreted fraction, in treated and non-treated cells, by employing qPCR and ELISA, respectively. mRNA expression of both IL10 and IL1β were significantly elevated in untreated brainstem and cerebellar astrocytes isolated from SHR when compared to Wistar astrocytes. No changes were observed in the secreted fraction. While ACEA-treatment resulted in a significant increase in IL10 gene expression in Wistar brainstem astrocytes (Log2FC ≥ 1, p < 0.05), its effect in SHR brainstem astrocytes was diminished. Ang II treatment resulted in a strong inhibitory effect on IL10 gene expression in astrocytes from both brain regions of SHR and Wistar rats (Log2FC ≤ −1, p < 0.05), and an increase in IL1β gene expression in brainstem astrocytes from both strains (Log2FC ≥ 1, p < 0.05). Co-treatment of Ang II and ACEA resulted in neutralization of Ang II-mediated effect in Wistar brainstem and cerebellar astrocytes, but not SHR astrocytes. Neither Ang II nor ACEA resulted in any significant changes in IL10 or IL1β secreted proteins. These data suggest that Ang II and ACEA have opposing roles in the regulation of inflammatory gene signature in astrocytes isolated from SHR and Wistar rats. This however does not translate into changes in their secreted fractions.
Characterization of the CYP3A4 Enzyme Inhibition Potential of Selected Flavonoids
Authors: Kondža Martin , Bojić Mirza , Tomić Ivona , Maleš Željan , Rezić Valentina , Ćavar Ivan
Published: May 2021

Acacetin, apigenin, chrysin, and pinocembrin are flavonoid aglycones found in foods such as parsley, honey, celery, and chamomile tea. Flavonoids can act as substrates and inhibitors of the CYP3A4 enzyme, a heme containing enzyme responsible for the metabolism of one third of drugs on the market. The aim of this study was to investigate the inhibitory effect of selected flavonoids on the CYP3A4 enzyme, the kinetics of inhibition, the possible covalent binding of the inhibitor to the enzyme, and whether flavonoids can act as pseudo-irreversible inhibitors. For the determination of inhibition kinetics, nifedipine oxidation was used as a marker reaction. A hemochromopyridine test was used to assess the possible covalent binding to the heme, and incubation with dialysis was used in order to assess the reversibility of the inhibition. All the tested flavonoids inhibited the CYP3A4 enzyme activity. Chrysin was the most potent inhibitor: IC50 = 2.5 ± 0.6 µM, Ki = 2.4 ± 1.0 µM, kinact = 0.07 ± 0.01 min−1, kinact/Ki = 0.03 min−1 µM−1. Chrysin caused the highest reduction of heme (94.5 ± 0.5% residual concentration). None of the tested flavonoids showed pseudo-irreversible inhibition. Although the inactivation of the CYP3A4 enzyme is caused by interaction with heme, inhibitor-heme adducts could not be trapped. These results indicate that flavonoids have the potential to inhibit the CYP3A4 enzyme and interact with other drugs and medications. However, possible food–drug interactions have to be assessed clinically.