bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon
Authors: Suriyani Irma , Kadriah Ince Ayu Khairana , Kuruseng Ilmiah
Published: February 2017

ABSTRACT This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 106 cfu/mL of V. harveyi by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA Vibrio were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of Vibrio was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on Vibrio pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of V. harveyi. Keywords: specific primer, luminous Vibrio bacteria, pathogenic, PCR method, hemolysin gene  ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan atau sensitivitas primer hemolisin dalam mendeteksi Vibrio patogen dengan lama pemaparan berbeda. Penelitian ini dilakukan dengan menginfeksikan Vibrio harveyi pada benur udang dengan metode perendaman pada konsentrasi 106 cfu/mL. Pengambilan sampel dilakukan pada waktu tiga, enam, 12, 24, 48, dan 72 jam pascainfeksi. Keberadaan gen hemolisin pada bakteri V. harveyi dideteksi menggunakan teknik polymerase chain reaction (PCR). Hasil elektroforesis memperlihatkan bahwa pada pemaparan tiga dan enam jam keberadaan gen hemolisin dari bakteri Vibrio patogen yang diinfeksikan sudah dapat terdeteksi pada benur walaupun masih terlihat tipis. Pada pemaparan 12 jam terlihat sangat jelas pita-pita DNA dari bakteri patogen. Sedangkan pada pemaparan 24, 48, dan 72 jam sudah tidak terdeteksi lagi gen hemolisin dari bakteri Vibrio. Hal ini diduga disebabkan terjadinya penurunan populasi bakteri Vibrio yang hidup dalam tubuh dan media pemeliharaan udang. Pentingnya deteksi cepat diawal udang terinfeksi bakteri (3–12 jam) karena setelah 12 jam infeksi sudah sulit untuk mendeteksi keberadaan bakteri patogen di dalam tubuh udang. Kata kunci: primer spesifik, bakteri Vibrio berpendar, patogenik, metode PCR, gen hemolisin
GIFT Nile tilapia Oreochromis niloticus fed on diet containing probiotic
Authors: Soedibya Petrus Hari Tjahja
Published: February 2017

ABSTRACT This research was conducted to evaluate the effect of the diet containing probiotic on Nile tilapia, Oreochromis niloticus. Fish with an initial body weight of 3.62±1.33 g were fed on diet supplemented with either 0%, 10%, 15%, 20% of probiotic. Results showed that fish fed on the diet supplemented with 15% of probiotic had the highest protein retention and specific growth rate, which were16.48±0.49% and 17.65±0.9%/day respectively. Keywords: probiotic, Azola meal, protein retention, specific growth rate  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efek pemberian pakan mengandung probiotik pada ikan nila, Oreochromis niloticus. Ikan dengan bobot awal 3,62±1,33 g diberi pakan mengandung 0%, 10%, 15%, 20% probiotic. Hasil penelitian menunjukkan bahwa ikan yang diberi pakan mengandung 15% probiotik memiliki retensi protein dan laju pertumbuhan tertinggi masing-masing dengan nilai 16,48±0,49% dan 17,65±0,9%/ hari. Kata kunci: probiotik, tepung Azola, retensi protein, laju pertumbuhan harian
Survival and growth of catfish Pangasionodon sp. larvae fed on vitamin C-enriched Artemia
Authors: Setiawati Mia , Putri Darina , Jusadi Dedi
Published: February 2017

ABSTRACT The research was conducted to evaluate effect of the administration of Artemia enriched with vitamin C on the survival, total body length, and average body weight of Pangasionodon sp. larvae. Artemia were enriched with either 0, 50, 100, or 150 mg/L vitamin C immersed in the culture medium for 13 hours. Two days old larvae were fed on enriched Artemia 12 times/day for seven days. At the end of feeding experiment, it was found that fish fed on Artemia enriched with 100 mg/L culture medium had the highest survival, total length and body weight, respectively 76.17±12.78%, 1.63±0.34 cm, and 20.6±3.8 mg. Keywords: survival, growth, Artemia, vitamin C, catfish, Pangasionodon sp.  ABSTRAK Penelitian ini dilakukan untuk mengetahui pengaruh Artemia yang diperkaya dengan vitamin C dosis berbeda terhadap kelangsungan hidup, panjang total, dan bobot tubuh larva ikan patin Pangasionodon sp. Pada penelitian ini, Artemia diperkaya dengan vitamin C dosis 0, 50, 100, atau 150 mg/L media pengayaan. Larva yang berumur dua hari diberi pakan Artemia hasil pengayaan sebanyak 12 kali sehari selama tujuh hari masa pemeliharaan. Pada akhir percobaan, didapatkan bahwa larva ikan yang diberi Artemia diperkaya vitamin C dosis 100 mg/L media pengayaan memberikan kelangsungan hidup 76,17±12,78%, panjang total 1,63±0,34 cm, dan bobot tubuh paling tinggi 20,6±3,8 mg. Kata kunci: kelangsungan hidup, pertumbuhan, Artemia, vitamin C, ikan patin, Pangasionodon sp.
Digestibility of pre-treated cassava peel as feed ingredient for Nile tilapia
Authors: Mulyasari , , Kurniawati Feri , Setiawati Mia
Published: February 2017

ABSTRACT This research aimed at determining digestibility coefficient of cassava peel(Manihot utilissima) after immersion in 3% (w/v) NaOH for three days, fermentation using combined fungi of 10% Trichoderma viride and Phanerochaete chrysosporium for seven days, and fermentation using 15% (w/w) Bacillus megaterium for five days as feed ingredients for Nile tilapia Oreochromis niloticus. Total digestibility test was conducted by mixing 30% of cassava peel and 70% of reference diet. Nile tilapia at the average weight of 16.6 g were used as experimental fish. Fish was held for 28 days in aquarium (50x50x50 cm3) at the density of 10 fish/aquarium. Fish were fed twice daily to satiation. Feces collection started after five days of adaptation to chromium oxide diets. The results showed that the three treatments had significant effects compared to control (P<0.05), protein digestibility of were improved 5%, 15%, and 10%, energy digestibilitiy were 20%, 18%, 16%, and total digestibility of test cassava peel were 174%, 151%, and 164%, respectively. Cassava peel fermented with combined 10% mold showed the highest protein digestibility impliying it potency as feed ingredient for Nile tilapia diet. Keywords: Nile tilapia, cassava peel, NaOH, mold, bacteria, digestibility  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kecernaan kulit ubi kayu (Manihot utilissima) setelah perendaman dengan NaOH 3% (w/v) selama tiga hari, fermentasi kapang Trichoderma viride dan Phanerochaete chrysosporium 10% (w/w) selama tujuh hari, dan fermentasi bakteri Bacillus megaterium 15% (w/w) selama lima hari sebagai bahan baku pakan ikan nila. Uji kecernaan total bahan dilakukan dengan mencampurkan 30% kulit ubi kayu dengan 70% pakan acuan. Ikan uji yang digunakan adalah ikan nila dengan bobot rata-rata 16,6 g. Ikan dipelihara selama 28 hari dengan kepadatan 10 ekor/akuarium berukuran 50x50x50 cm3. Pemberian pakan dilakukan dua kali sehari secara at satiation. Pengumpulan feses dimulai setelah lima hari adaptasi pakan uji yang diberi indikator kromium oksida. Hasil penelitian menunjukkan bahwa ketiga perlakuan memberikan pengaruh yang berbeda nyata terhadap kontrol (P<0,05) dan meningkatkan nilai kecernaan protein pakan berturut-turut sebesar 5%, 15%, dan 10%, nilai kecernaan energi sebesar 20%, 18%, dan 16%, serta nilai kecernaan total bahan sebesar 174%, 151%, dan 164%. Perlakuan kulit ubi kayu yang difermentasi dengan kapang menunjukkan nilai kecernaan protein pakan yang tertinggi sehingga berpotensi sebagai bahan baku pakan ikan nila. Kata kunci: ikan nila, kulit ubi kayu, NaOH, kapang, bakteri, kecernaan
Prevention of Aeromonas hydrophila on catfish juvenile using garlic and shatterstone herb
Authors: Wahjuningrum Dinamella , Astrini Retno , Setiawati Mia
Published: February 2017

ABSTRACT

 

This study aimed to determine the dose of garlic and shatterstone herb mixed into the feed in 11-day old catfish juvenile (body length 1.53±0.26 cm, body weight of 40±16 mg). The study was divided into two steps, the first step was dose determination and the second step was the dose testing. The treatment was carried out for 21 days, then the challenge test was conducted by immersion method for 60 minutes in A. hydrophila density of 104 cfu/mL. Parameters of observation were survival, feed intake, relative growth rate, morphology of the liver, and water quality. The result showed that garlic at 25 g/kg and shatterstone herb at 5 g/kg provided higher fish viability (81.11±3.85%) compared with the control (23.00±5.77%). Feed intake and relative growth rate were not different among the treatments. Whereas the liver condition in positive control was paler than other treatments. Thus, prevention of A. hydrophila infection on catfish juvenile was effectively achieved by feeding with diet supplemented by garlic 25 g/kg and shatterstone herb 5 g/kg.

 

Keywords: garlic, shatterstone herb, Aeromonas hydrophila, juvenile, catfish

 

 

ABSTRAK

 

 Penelitian ini bertujuan untuk menentukan dosis bawang putih dan meniran yang dicampur ke pakan dalam bentuk tepung pada benih lele berumur 11 hari (berukuran panjang 1,53±0,26 cm dan bobot 40±16 mg). Penelitian ini terbagi menjadi dua tahap, pertama adalah tahap penentuan dosis (dosis 20 ppt bawang putih+5 ppt meniran dan 25 ppt bawang putih+5 ppt meniran) dan kedua adalah tahap pengujian dosis. Perlakuan diberikan selama 21 hari, kemudian uji tantang dilakukan dengan metode perendaman selama 60 menit dengan kepadatan bakteri A. hydrophila 104 cfu/mL. Parameter yang diamati adalah kelangsungan hidup, jumlah konsumsi pakan, pertumbuhan relatif, morfologi organ hati, dan kualitas air. Hasil penelitian membuktikan bahwa perlakuan bawang putih 25 g/kg dan meniran 5 g/kg memberikan kelangsungan hidup benih lele sebesar 81,11±3,85%, sedangkan kontrol positif 23,00±5,77%. Jumlah konsumsi pakan dan pertumbuhan relatif tidak berbeda antarperlakuan, sedangkan organ hati berwarna pucat pada kontrol positif. Pencegahan infeksi bakteri A. hydrophila dengan pakan campuran bawang putih 25 g/kg dan meniran 5 g/kg efektif dilakukan pada benih ikan lele.

 

Kata kunci: bawang putih, meniran, Aeromonas hydrophila, benih, ikan lele

 
The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.
Authors: Wahjuningrum Dinamella , Ikhsan Muharram Nur , Sukenda , , Evan Yan
Published: February 2017

ABSTRACT  Target production of catfish in aquaculture can be reached by suppressing the pathogenic bacterial infection. Previous works had shown that turmeric Curcuma longa has antibacterial activity. The objectives of this research were to determine the best method of extraction and to evaluate the efficacy of turmeric extract as feed additive to control Edwardsiella tarda disease in catfish culture. Briefly, the objective was achieved through in vitro assay based on inhibition ability of extraction method against E. tarda, while the following objective was obtained through in vivo assay based on their survival during challenge test either as preventive or curative measurement. A complete randomized design with three replications was used for each assay. Furthermore, challenge test was done by mean intraperitoneal injection at concentration 106 cfu/mL of E. tarda (LD50). The results showed that 15 minutes decoction method allowed the best inhibition with diameter 7.42 mm of clear zone and then curative measurement using turmeric extract could be the best application against E. tarda since it gave 86.67% of survival. Clinical signs such as swelling, hemoraghic, body ulcer, gastroentritis and gaseous captivity were observed on challenged fish. However, there were no significant different among treatments for specific growth, body weight, and absolute length parameters.  Keywords: Edwardsiella tarda, extraction, turmeric, catfish, inhibition zone  ABSTRAK Peningkatan produksi ikan lele dapat dicapai melalui pencegahan infeksi penyakit bakterial. Hasil beberapa penelitian membuktikan bahwa kunyit Curcuma longa terbukti memiliki zat aktif yang bersifat antibakteri. Penelitian ini bertujuan untuk mencari metode ekstraksi terbaik dan mengevaluasi efektivitas penambahan ekstrak kunyit pada pakan untuk pengendalian patogen Edwardsiella tarda pada ikan lele. Metoda ekstraksi kunyit diuji secara in vitro dengan metoda zona hambat, sedangkan efikasi diuji secara in vivo melalui perlakuan pencegahan dan pengobatan. Penelitian didesain dalam rancangan acak lengkap dengan tiga ulangan. Efikasi ekstrak kunyit diketahui dari nilai sintasan ikan lele hasil uji tantang E. Tarda melalui injeksi peritoneal dengan dosis 106 cfu/ mL (LD50). Hasil uji in vitro menunjukkan bahwa metode dekoksi selama 15 menit memberikan zona hambat terbaik yaitu sebesar 7,42 mm. Hasil uji in vivo menunjukkan bahwa ekstrak kunyit sebagai tindak pengobatan memberikan nilai sintasan terbaik, yaitu sebesar 86,67%. Ikan lele yang diuji tantang menunjukkan gejala klinis berupa pembengkakan, luka, gastroentritis, dan gas pada perut. Tidak terdapat perbedaan nyata di antara perlakuan untuk parameter laju pertumbuhan harian, bobot harian, dan pertumbuhan panjang mutlak. Kata kunci: Edwardsiella tarda, ekstraksi, kunyit, ikan lele, zona hambat
Acute and sublethal toxicity of copper in Siam-catfish juvenile Pangasianodon hypophthalmus
Authors: Sihono Dody , Supriyono\ Eddy , Setiawati Mia
Published: February 2017

ABSTRACT This study was aimed to determine the level of acute toxicity (LC50) and to analyze the effect of Cu on the survival, growth, haematological, and bioaccumulation of Cu in Pangasianodon hypophthalmus juvenile at sublethal conditions. The experiment trial used completely randomized design with five concentration treatments and two replications to determine LC50. The Cu concentration used were 0, 0.2, 0.7, 1.2, and 1.7 ppm. Sublethal treatment used four Cu concentrations with three replicates. The concentrations were 0, 0.167, 0.334 and 0.667 ppm. Experimental fish used had an average total length 11.0±1.7 cm and weight 13.00±1.72 g. Results showed that Cu was highly toxic to P. hypophthalmus with a LC50-96 hours value of 0.667 ppm (0.539–0.805 ppm). At sublethal concentrations, Cu significantly decreased survival and growth, and increased Cu accumulation started from concentration of 0.167 ppm. Bioaccumulation of Cu started from the highest were in liver, gills, skin and flesh, respectively. Decreased of erythrocytes, haemoglobin and hematocrit indicated anemia, while increased of leukocytes indicated infection and physical stress on the body tissues. Keywords: copper toxicity, haematological, bioaccumulation, Siam-catfish juvenile  ABSTRAK Penelitian ini bertujuan untuk menentukan tingkat toksisitas akut (LC50) dan menganalisis pengaruh Cu terhadap sintasan, pertumbuhan, kondisi hematologi, dan akumulasi Cu pada juvenil ikan patin siam (Pangasianodon hypophthalmus) pada kondisi subletal. Rancangan penelitian yang digunakan adalah lima perlakuan konsentrasi dengan dua ulangan untuk menentukan LC50. Konsentrasi Cu yang digunakan adalah 0; 0,2; 0,7; 1,2; dan 1,7 ppm. Pemeliharaan subletal menggunakan empat variasi konsentrasi dengan tiga ulangan. Konsentrasi Cu yang digunakan adalah 0; 0,167; 0,334; dan 0,667 ppm. Ikan uji yang digunakan memiliki panjang total rata-rata 11,0±1,7 cm dan berat 13,00±1,72 g. Hasil menunjukkan bahwa Cu sangat toksik terhadap juvenil P. hypophthalmus dengan nilai LC50-96 jam sebesar 0,667 ppm (0,539–0,805 ppm). Pada konsentrasi subletal, Cu berpengaruh nyata terhadap penurunan sintasan dan pertumbuhan serta menyebabkan peningkatan akumulasi Cu mulai pada konsentrasi 0,167 ppm. Bioakumulasi Cu secara berurutan mulai dari yang tertinggi yaitu pada hati, insang, kulit dan daging. Penurunan eritrosit, hemoglobin dan hematokrit menunjukkan terjadinya anemia, sementara peningkatan jumlah leukosit menunjukkan infeksi dan stres fisik pada jaringan tubuh. Kata kunci: toksisitas tembaga, hematologi, bioakumulasi, bioakumulasi, sintasan, juvenil patin siam
Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method
Authors: Sukenda , , Febriansyah Trian Rizky , Nuryati Sri
Published: February 2017

ABSTRACT The study was aimed to evaluate the efficacy of formalin-killed non-hemolytic Streptococcus agalactiae N14G and NK1 isolates whole-killed vaccine to prevent streptococcosis in tilapia. Ten fishes were reared in a tank 60x30x35 cm3 with an average body weight at 10.79±0.99 g. Fish was vaccinated through bath immersion at a concentration of 109 cfu/mL. Fish was subsequently challenged by intraperitonial injection of Streptococcus agalactiae 105 cfu/mL at 11 days post-vaccination. Parameters observed were survival, relative percent survival (RPS), total leukocyte, phagocytic activity, antibody titer, total erythrocyte, haemoglobin level, haematocrit level, dan water quality. Samplings were performed in day-0, 20, and 30 after vaccination. Both vaccines have shown higher survival (60%) and RPS (40%) when challenged with pathogenic Streptococcus N14G isolates than other treatments. Based on RPS percentage observed, those vaccine were still not sufficiently effective to combat S. agalactiae infection. Keywords: tilapia, bath immersion, Streptococcus agalactiae, whole-cell vaccine ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efikasi vaksin formalin-killed cell Streptococcus agalactiae tipe isolat nonhemolitik N14G dan NK1 se utuh yang diberikan melalui perendaman dalam mencegah penyakit streptococcosis pada ikan nila. Ikan nila yang digunakan memiliki bobot 10,79±0,99 g, dipelihara sebanyak sepuluh ekor dalam akuarium ukuran 60x30x35 cm3. Ikan divaksinasi dengan metode perendaman dengan dosis 109 cfu/mL. Uji tantang dilakukan pada hari ke-11 pascavaksinasi dengan dosis 105 cfu/mL. Parameter yang diamati meliputi sintasan (SR), sintasan relatif/relative percent survival (RPS), total leukosit, aktivitas fagositik, titer antibodi, total eritrosit, kadar hemoglobin, kadar hematokrit, dan kualitas air. Pengamatan parameter dilakukan pada hari ke-0, ke-10, ke-20, dan ke-30. Hasil penelitian menunjukkan perlakuan kedua vaksin yang diinfeksi bakteri patogen isolat N14G memberikan nilai sintasan dan nilai RPS tertinggi dibanding perlakuan lainnya. Nilai sintasan dan RPS kedua perlakuan tersebut adalah 60% dan 40%. Nilai RPS yang cukup kecil menunjukkan vaksin yang diberikan masih kurang efektif untuk mencegah infeksi bakteri S. agalactiae. Kata kunci: ikan nila, perendaman, Streptococcus agalactiae, vaksin sel utuh
The growth and meat quality of Siamese catfish fed different level of protein
Authors: Purnomo Novieanto , Utomo Nur Bambang Priyo , Azwar Zafril Imran
Published: February 2017

ABSTRACT This study was to evaluate growth performance and meat quality of Siamese catfish Pangasianodon hypophthalmus fed on commercial diets with different protein levels. Fish with average initial body weight of 33,61 g were reared in hapa (2×2×1 m3) at density of 30 ind/hapa. Fish were hand-fed with experimental diet to satiety twice daily for 60 days. Experimental design was set in completely randomized design. Each treatment was done in triplicates. Experimental diets were a commercial diet to contain four differennt levels of protein; i.e. diet A (18%), diet B (23%), diet C (28%), and diet D (32%). Specific growth rate (SGR), feed conversion ratio (FCR), protein (RP) and lipid (RL) retention, hepatosomatic index (HSI), lipid and glycogen content of liver, protein and lipid content of meat, edible portion, and fillet textural were calculated. The results of the experiment showed that the highest weight gain and SGR, lowest FCR and meat protein were obtained in the group of fish fed 23%–32% protein diets. In conclusion, 23% protein diets gave the best growth performance and meat quality of Siamese catfish. Keywords : growth performance, meat quality, commercial diet, Pangasianodon hypophthalmus  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan kualitas daging ikan patin siam Pangasianodon hypophthalmus yang diberi pakan komersial dengan kandungan protein berbeda. Ikan patin yang digunakan dengan bobot awal 33,61 g ditebar sebanyak 30 ekor/hapa ukuran 2×1×1 m3 dan ikan diberi pakan percobaan dua kali sehari secara at satiation selama 60 hari. Penelitian ini didesain dalam rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Pakan yang digunakan adalah pakan komersial dengan empat kandungan protein yang berbeda: pakan A (18%), pakan B (23%), pakan C (28%), dan pakan D (32%). Parameter uji pada penelitian ini adalah laju pertumbuhan spesifik (SGR), rasio konversi pakan (FCR), retensi protein (RP), retensi lemak (RL), indeks hepatosomatik (HSI), lemak dan glikogen hati, protein dan lemak daging, serta tekstur daging. Hasil penelitian menunjukan bahwa penambahan bobot tubuh, SGR tertinggi, FCR terendah, dan protein daging diperoleh pada perlakuan protein pakan 23%–32%. Hasil penelitian ini menunjukkan bahwa perlakuan protein pakan 23% memberikan kinerja pertumbuhan dan kualitas daging ikan terbaik. Kata kunci: kinerja pertumbuhan, kualitas daging, pakan komersial, Pangasianodon hypophthalmus
Induction of gonadal maturation of eel using PMSG, antidopamine, and estradiol-17β
Authors: Tomasoa Aprelia Martina , Sudrajat Agus Oman , Junior Muhammad Zairin
Published: February 2017

ABSTRACT The study was aimed to induce gonadal maturation of eel Anguilla bicolor bicolor by hormonal treatment using pregnant mare serum gonadotropin (PMSG), antidopamine (AD), dan estradiol-17β (E2). The research used complete randomized design with five hormone combination treatments consisted of PK (NaCl 0.95%) as control, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), and P20BE (PMSG 20 IU + AD 10 ppm + E2 150 µg), with three individual replications for each treatment. Hormonal induction was applied through intramuscular injection weekly during eight weeks at initial body weight of 200 g. The result showed that P10BE treatment has obtained highest level on E2 (0.43 ng/mL), FSH (2.68 mIU/mL) has increased in week-4 and level on T (1.2 ng/mL), LH (2.80 mIU/mL) has increased in week-8. P10BE has affected spermatogenesis and the increased of GSI (2.46%) in fourth and sixth week compared to PK (1.28%), P10A (1.58%), P20A (1.34%), and P20BE (2.12%). In conclusion, combination of PMSG, AD, and E2 hormones could stimulate the gonadal maturation of eel at the size of 200 g into male. Keywords: Anguilla bicolor bicolor, gonadal growth, hormone, maturation  ABSTRAK Penelitian ini dilakukan untuk menginduksi pematangan gonad ikan sidat Anguilla bicolor bicolor secara hormonal dengan menggunakan pregnant mare serum gonadothropin (PMSG), antidopamin (AD), dan estradiol-17β (E2). Metode penelitian ini menggunakan metode eksperimen rancangan acak lengkap dengan lima perlakuan kombinasi hormon, yaitu PK (larutan NaCl 0,95%) sebagai kontrol, P10A (PMSG 10 IU + AD 10 ppm), P20A (PMSG 20 IU + AD 10 ppm), P10BE (PMSG 10 IU + AD 10 ppm + E2 150 µg), dan P20BE (PMSG 10 IU+AD 10 ppm+E2 150 µg), dengan tiga ulangan individu pada masing-masing perlakuan. Induksi hormonal dilakukan dengan metode penyutikan secara intramuskuler setiap minggu selama delapan minggu dengan bobot ikan yang berukuran 200 g. Hasil penelitian menunjukkan bahwa analisis hormon pada perlakuan P10BE memberikan nilai tertinggi masing-masing; kadar E2 (0,43 ng/mL), kadar FSH (2,68 mIU/mL) meningkat di minggu keempat dan kadar T (1,2 ng/mL), kadar LH (2,80 mIU/mL) mengalami peningkatan pada minggu kedelapan. P10BE memberikan efek pada spermatogenesis dan peningkatan nilai GSI (2,46%) pada minggu keempat sampai keenam selama penyuntikkan dibandingkan dengan PK (1,28%), P10A (1,58%), P20A (1,34%) dan P20BE (2,12%). Dengan demikian, kombinasi hormon PMSG, AD, dan E2 dapat merangsang perkembangan dan mempercepat pematangan gonad ikan sidat ukuran 200 g menjadi jantan. Kata kunci: Anguilla bicolor bicolor, pertumbuhan gonad, hormon, maturasi
Effectivity of Musa paradisiaca extract to control Saprolegnia sp. infection on giant gourami larvae
Authors: Nuryati Sri , Aulia Nadia , Rahman ,
Published: February 2017

ABSTRACT Larval stage of giant gourami is a critical period due to fungal infection, such as Saprolegnia sp. infection. There are some plants which have antiseptic compound like banana Musa paradisiaca. This research was aimed to examine the effectiveness of the banana stem extract M. paradisiaca to control Saprolegnia sp. infection on giant gurami larvae through immersion. Eight-day old gorami larvae (at the initial of 0.5±0.03 cm) was reared in an aquarium sized 25×25×25 cm3 at the density of 8 fry/L. Culture media were added banana stem extract at the dose of 0; 0.08; 0.12; and 0.16 g/L during 21 days of rearing period. Challenge test was performed for 14 days by giving Saprolegnia sp. spores at the density of 104 cells/mL and banana stem extract. The treatment dose of 0.16 g/L has showen survival 100% than positive control  after the challenge test. Keywords: giant gourami, Musa paradisiaca, Saprolegnia sp., fry  ABSTRAK Fase larva ikan gurami merupakan masa kritis terhadap infeksi cendawan, seperti jenis Saprolegnia sp. Beberapa tanaman memiliki daya antiseptik seperti tanaman pisang ambon Musa paradisiaca. Penelitian ini bertujuan untuk menguji efektivitas ekstrak batang pisang ambon M. paradisiaca dalam mengurangi infeksi Saprolegnia sp. pada larva ikan gurami melalui media pemeliharaan. Larva gurami umur delapan hari (panjang larva 0,5+0,03 cm) dipelihara pada akuarium berukuran 25×25×25 cm3 dengan padat tebar 8 ekor/L. Media pemeliharaan diberi ekstrak batang pisang ambon dosis 0; 0,08; 0,12; dan 0,16 g/L selama 21 hari. Uji tantang dilakukan selama 14 hari dengan pemberian spora Saprolegnia sp. kepadatan 104 sel/mL dan ekstrak batang pisang ambon. Perlakuan dosis 0,16 g/L memberikan kelangsungan hidup sebesar 100% yang lebih tinggi dibandingkan perlakuan kontrol positif setelah uji tantang. Kata kunci: giant gourami, Musa paradisiaca, Saprolegnia sp., larva
Growth and survival of Kryptopterus lais treated with thyroxine hormone
Authors: Agusnimar , , Rosyadi ,
Published: February 2017

ABSTRACT This experiment was conducted to evaluate the effect of different doses of thyroxine supplemented in commercial feed (pellet 781-1, produced by Choroend Pokhpand Indonesia) on growth and survival of Kryptopterus lais. Adult K. lais with initial body weight of 43−99 g were used as samples. A completely randomized designed with four treatments and three replications were used in this experiment. Four doses of thyroxine used as treatment were 0,0 mg/kg (P1); 0.08 mg/kg (P2); 0.10 mg/kg (P3); and 0.12 mg/kg (P4). Fish were fed 10% of body weight daily, given twice per day. The results showed that the treatement of P2 provided the best growth of absolute body weight and length of K. lais, the growth of absolute weight was 14.8 mg while the growth of absolute length was 1.1 cm.The best daily growth of body weight founded at P4 (0.27 %), while the best daily growth of body length were founded at P2 and P4 (0.06%). The survival of fish were 100% in all treatments. Keywords: Kryptopterus lais, thyroxine hormone, growth, survival  ABSTRAK Penelitian ini dilakukan dengan tujuan untuk mengevaluasi pengaruh dosis hormon tiroksin dalam pakan ikan terhadap pertumbuhan dan kelangsungan hidup calon induk ikan selais. Ikan uji yang digunakan dalam penelitian ini memiliki bobot 43−99 g. Rancangan penelitian yang digunakan adalah rancangan acak lengkap satu faktor dengan empat perlakuan dan tiga ulangan. Dosis hormon tiroksin yang digunakan pada masing-masing perlakuan adalah 0,0 mg/kg (P1); 0,08 mg/kg (P2); 0,10 mg/kg (P3); dan 0,12 mg/kg (P4). Pakan yang diberikan pada ikan uji adalah pelet 781-1 yang diproduksi oleh Charoen Pokphand Indonesia. Setelah dilakukan penelitan diperoleh data bahwa pertumbuhan bobot mutlak dan panjang mutlak tertinggi ditemui pada perlakuan P2, masing-masing 14,8 g dan 1,1 cm. Laju pertumbuhan bobot harian tertinggi terdapat pada perlakuan P4 (0,27%) dan laju pertumbuhan  panjang harian tertinggi terdapat pada perlakuan P2 dan P4 (0,06%). Tingkat kelangsungan hidup ikan uji selama penelitian 100%. Kata kunci: Kryptopterus lais, hormon tiroksin, pertumbuhan, kelangsungan hidup
Optimum salinity for growth of mangrove crab Scylla serrata seed in recirculation systems
Authors: Hastuti Yuni Puji , Affandi Ridwan , Safrina Mafatih Devi , Faturrohman Kurnia , Nurussalam Wildan
Published: February 2017

ABSTRACT One of the abiotic factors that affects the growth and the survival of crabs is salinity. The optimum salinity media will give maximum impact on mangrove crab Scylla serrata due to the osmoregulation process. This study aimed to examine the effect of salinity on the survival rate (SR) and spesific growth rate (SGR) of mangrove crab through the reaction of physiological condition. The treatments were rearing mangrove crab at the salinity medium of 15 ppt (A), 20 ppt (B), 25 ppt (C), and 30 ppt (D). Result showed that different salinity performed a significant effect (P<0.05) on the survival rate and specific growth rate of the crabs. The low level of stress, shown by the high value of total hemocyte and the low osmotic pressure, has made salinity of 25 ppt was the optimum condition for the mangrove crab rearing. Keywords: salinity, survival, specific growth rate, mangrove crab  ABSTRAK Salah satu faktor abiotik yang memengaruhi pertumbuhan dan kelangsungan hidup kepiting adalah salinitas. Salinitas media optimum akan memberikan efek yang maksimal pada kepiting bakau Scylla serrata sehubungan dengan proses osmoregulasi tubuhnya. Penelitian ini bertujuan untuk menguji pengaruh salinitas pada kelangsungan hidup dan laju pertumbuhan spesifik kepiting bakau melalui reaksi kondisi fisiologis. Penelitian ini terdiri atas perlakuan salinitas media 15 ppt (A), 20 ppt (B), 25 ppt (C), dan 30 ppt (D). Hasil penelitian menunjukkan bahwa perbedaan salinitas media pemeliharaan kepiting bakau memberikan perbedaan nyata (P<0,05) pada kelangsungan hidup dan laju pertumbuhan spesifik kepiting bakau. Rendahnya tingkat stres pada salinitas 25 ppt dijelaskan dengan tingginya jumlah total hemosit dan rendahnya tekanan osmotik sehingga salinitas 25 ppt merupakan kondisi optimum bagi pemeliharaan kepiting bakau. Keywords: salinitas, kelangsungan hidup, laju pertumbuhan spesifik, kepiting bakau
Sex Reversal of Red Tilapia (Oreochromis sp.) by Larval Immersion using Aromatase Inhibitor
Authors: Sudrajat M. Agus , Astutik I.D. , Arfah H.
Published: February 2017

This study was performed to verify the effect of red Nile tilapia (Oreochromis sp.) larval immersion using aromatase inhibitor (1,3-Diaza-2,4-Cyclopentadience) on percentage of male fish.  Nine-day-old of Nile tilapia larva was immersed in aromatase inhibitor at the dose of 0, 10, 20 and 30 mg/L water for 10 hours.  Number of larva immersed was 100 fish per treatment.  The results of study indicated that immersing of 9-day-old larva for 10 hours was ineffective in producing male fish. The highest male percentage was 59.5% at the dose of 20 mg/L, and statistically similar with other treatments including control.  However, this treatment has possibility to produce hermaphrodite, and had no effect on survival of fish.

Keywords: red Nile tilapia, Oreochromis, Aromatase Inhibitor, immersion

 

ABSTRAK

Penelitian ini dilakukan untuk mengetahui efek perendaman ikan nila merah (Oreochromis sp.) menggunakan aromatase inhibitor (1,3-Diaza-2,4-Cyclopentadience) terhadap persentase ikan jantan.  Larva ikan nila umur 9 hari direndam dengan aromatase inhibitor dengan dosis 0, 10, 20 dan 30 mg/L air selama 10 jam.  Jumlah larva yang direndam sebanyak 100 ekor per perlakuan.  Hasil penelitian menunjukkan bahwa perendaman pada fase larva umur 9 hari selama 10 jam terbukti kurang efektif untuk menghasilkan ikan jantan. Persentase jantan tertinggi hanya mencapai 59,5% dengan dosis 20 mg/L aromatase inhibitor dan tidak berbeda nyata dengan perlakuan lainnya termasuk kontrol. Namun perlakuan tersebut berpeluang menghasilkan individu hermaprodit, dan tidak berpengaruh terhadap kelangsungan hidup ikan.

Kata kunci: ikan nila merah, Oreochromis, Aromatase Inhibitor, perendaman
Requirement of n-6 and n-3 Fatty Acid in Broodstock Diets on Reproductive Performance of Green catfish, Hemibagrus nemurus Blkr.
Authors: Utiah Adharto , Junior M. Zairin , Mokoginta I. , Affandi R. , Sumantadinata K.
Published: February 2017

This experiment was conducted to determine the optimum dietary level of n-6 and n-3 fatty acids on reproduction performance of green catfish. Four experimental diets with different level of n-6 and n-3 fatty acid (diet A: 0.77% n-6 fatty acid, 0.07% n-3 fatty acid; diet B: 1.56%,0.10 %; diet C: 1.74%, 0.13 % and diet D: 2%, 0.28%) were used in this experiment during seven month. Diets given twice every day in the morning and evening. The 28 broodstock used in this research with density every waring seven broodstock. Samples of eggs were analyzed for fatty acid composition. The diameter of eggs, fecundity, hatching rate of the eggs, survival rate and percentage of abnormal larvae were determined. Results showed that different dietary level of n-6 and n-3 fatty acids of the broodstock significantly affect the reproductive performance especially fecundity and hatching rate of eggs. The maximum fecundity, egg diameter, and hatching rate were produced broodstock fed on 1.56% n-6 fatty acid and 0.10 % n-3 fatty acid in diet by at the level of 12.29% lipid.

Keywords : n-6 and n-3 fatty acids, reproduction performance, green catfish, Hemibagrus nemurus.

 

Abstrak

Penelitian ini dilakukan pada berbagai level asam lemak n-6 dan n-3 dalam pakan untuk melihat pengaruhnya terhadap penampilan reproduksi dari ikan baung. Penelitian dilaksanakan selama 7 bulan.  Empat jenis pakan  digunakan dalam percobaan ini dengan perbedaan kandungan asam lemak n-6 dan n-3 (pakan A: 0,77% asam lemak n-6, 0,56% asam lemak n-3; pakan B: 1,56%, 0,78%; pakan C: 1,74%, 1,00% and pakan D: 2,03%, 1,82%). Dalam penelitian ini 28 ekor induk digunakan dan ditebarkan kedalam waring dengan kepadatan 7 ekor tiap waring. Pakan diberikan pagi dan sore hari secara at satiation.  Sampel telur dan larva dianalisa komposisi asam lemak. Penampilan reproduksi yang diamati adalah diameter telur, fekunditas, derajat penetasan telur, derajat kelangsungan hidup larva dan persentase larva abnormal. Hasil penilitian menunjukan bahwa perbedaan kandungan asam lemak n-6 dan n-3 dalam pakan berpengaruh pada komposisi asam lemak n-6 dan n-3 telur dan juga memberikan pengaruh yang berbeda nyata terhadap penampilan reproduksinya khusus pada fekunditas dan derajat penetasan telur. Lama waktu matang yang dicapai oleh tiap induk relatif sama antar perlakuan.  Fekunditas, diameter telur, derajat penetasan telur dan derajat kelangsungan hidup larva tertinggi diperoleh pada induk yang memperoleh pakan yang mengandung 1,56% asam lemak n-6 dan 0,78% asam lemak n-3.

Kata kunci:  Asam lemak n-6 and n-3, penampilan reproduksi, ikan baung, Hemibagrus nemurus.
Assessing the behavior of machine learning methods to predict the activity of antimicrobial peptides
Authors: Camacho Francy Liliana , Torres-Sáez Rodrigo , Ramos-Pollán Raúl
Published: February 2017

This study demonstrates the importance of obtaining statistically stable results when using machine learning methods to predict the activity of antimicrobial peptides, due to the cost and complexity of the chemical processes involved in cases where datasets are particularly small (less than a few hundred instances). Like in other fields with similar problems, this results in large variability in the performance of predictive models, hindering any attempt to transfer them to lab practice. Rather than targeting good peak performance obtained from very particular experimental setups, as reported in related literature, we focused on characterizing the behavior of the machine learning methods, as a preliminary step to obtain reproducible results across experimental setups, and, ultimately, good performance. We propose a methodology that integrates feature learning (autoencoders) and selection methods (genetic algorithms) thorough the exhaustive use of performance metrics (permutation tests and bootstrapping), which provide stronger statistical evidence to support investment decisions with the lab resources at hand. We show evidence for the usefulness of 1) the extensive use of computational resources, and 2) adopting a wider range of metrics than those reported in the literature to assess method performance. This approach allowed us to guide our quest for finding suitable machine learning methods, and to obtain results comparable to those in the literature with strong statistical stability.
Citizen: An American Lyric - A Creative Rupture of Citizenship
Authors: Pinkoski Mary
Published: February 2017

This book review of Rankine's (2014) Citizen: An American Lyric explores how the book acts as a disruptive force for the reader, thereby calling to the surface and then rupturing deeply imbedded notions of what it means to be a citizen.
Temperature and Precipitation Trend Analysis over the Last 30 Years in Southern Tigray Regional State, Ethiopia
Authors: Hayelom Birhanu , Chen Yingjun , Marsie Zinabu , Negash Miseker
Published: February 2017

Long term Precipitation and temperature variations are one of the main determinants of climate variability of one’s area. The aim of this study is to determine trends variation in climatic elements of temperature and precipitation in the southern zone of Tigray regional state, Ethiopia. The station is assumed for the study of climatic records over southern zone of the region in detection for probable trends. The daily, monthly and annual precipitation totals and temperature observed at korem meteorological station were used for the period of 1981-2010 for Precipitation and 1985 – 2010 for minimum and maximum temperature. Summary of descriptive statistics and Mann Kendall test methods were employed for the observed data analysis to demonstrate any existence of possible trends. The main findings of the study indicated that the mean and maximum temperature had a general increasing trend; however, minimum temperature showed decreasing trend. In general annual temperature from 1985 – 2010 of the area showed a warming trend. Moreover analysis of the 30 years (1981-2010) annual precipitation showed a coefficient of variation ranging from 33.77 – 233 %. It indicated that the precipitation dissemination is not normal with large year to year variances.
Pengembangan Kelembagaan Pembibitan Ternak Sapi Melalui Pola Integrasi Tanaman-Ternak
Authors: Setiani Cahyati , Prasetyo Teguh
Published: February 2017

Pengkajian mengenai Pengembangan Pembibitan Ternak Sapi dilakukan di Desa Karangjambe, Kecamatan Wanadadi, Kabupaten Banjarnegara dan Desa Kalipoh, Kecamatan Ayah, Kabupaten Kebumen tahun 2004. Tujuan pengkajian adalah untuk mendapatkan model pembibitan ternak sapi yang sesuai dengan kondisi sumberdaya alam, sumberdaya manusia dan budaya yang berkembang di masyarakat perdesaan. Metode pengkajian yang digunakan adalah survey baik melalui wawancara dengan responden dan key informan  maupun observasi lapang.  Responden adalah anggota kelompok tani Bumi Mulyo (Kabupaten Banjarnegara) dan Becik Asih (Kabupaten Kebumen). Key informan adalah pengurus kelompok tani, aparat desa, serta petugas yang terkait dengan pengembangan pembibitan ternak sapi. Pola pengembangan usaha pembibitan dilakukan melalui bantuan pinjaman langsung masyarakat (BPLM) dan integrasi tanaman-ternak.  Sumberdaya alam di Desa Karangjambe didominasi tanah alluvial dan grumosol yang lahan pertanian utamanya berupa lahan sawah dengan pola tanam padi-padi-palawija, sedangkan di Desa Kalipoh didominasi tanah alluvial, lahan kering dengan pola tanam jagung-jagung+ketela pohon. Kelembagaan pembibitan sapi yang berkembang di kelompok tani Bumi Mulyo adalah: jangka waktu pelunasan 4 tahun, mengembalikan  pinjaman pokok (ternak bibit+konsentrat) tanpa bunga. Apabila  pedet yang dihasilkan betina, pada umur 15 sampai 18 bulan digulirkan kepada anggota lain, namun  jika yang dihasilkan pedet jantan, hasil penjualan dibagi 50% : 50%, masing-masing untuk penggaduh dan kelompok, sedangkan di kelompok tani Becik Asih jangka waktu pelunasan 3 tahun, peternak berhak atas ternak pokok dan  70% hasil penjualan pedet yang dihasilkan,  berkewajiban mengembalikan  pinjaman pokok untuk pembelian ternak bibit dengan jumlah sesuai dengan akad kredit.

 

Kata kunci: kelembagaan, pembibitan ternak sapi, integrasi tanaman-ternak
Nilai Lokal Krik Salamat sebagai Asas dalam Pembangunan Hukum
Authors: Zuhri Lahmuddin
Published: February 2017

Perpaduan nilai kearifan Lokal dan dan  Pancasila sebagai acuan pembangunan hukum yang krakter, mengingat begitu besar dan maje­muknya negara Indonesia. Tiap-tiap daerah mempunyai budaya dan agama yang terefleksikan pada kebijakan pembangunan. Kebijakan pembangunan yang terbentuk dari aspirasi masyarakat (botton up) akan memberikan kenyamanan bagi masyarakat dalam menyelenggarakan kehidupan yang berlandaskan nilai-nilai moralitas berkeadilan. Pembangunan hukum merupakan alat untuk melakukan perubahan sosial, yang mengedepankan nilai lokal yang dilaksanakan secara demokrasi dengan mekanisme permusyawaratan dengan asas rasionalitas dan keadilan sosial, asas berkelanjutan, serta asas persamaan hak. Nilai Kerik Salamat masyarakat Sumbawa, memandang bahwa kekuasaan itu datang dari Tuhan yang harus dipertangungjawabkan secara transparan, kepada Tuhan dan manusia.Kata Kunci: Nilai Lokal, Asas hukum, Pembangunan Hukum
Physiological Responses to Soil Drying by Warm‐Season Turfgrass Species
Authors: Zhang Jing , Kenworthy Kevin , Unruh J. Bryan , Poudel Bishow , Erickson John E. , Rowland Diane , Kruse Jason
Published: February 2017

A study describing the overall physiological responses to drought and exploring the underlying mechanisms in multiple turfgrass species and genotypes is needed to make improvements in breeding for turfgrass species that are tolerant to water‐limiting conditions. The objective of this study was to compare the differential canopy and physiological responses of 14 genotypes of warm‐season turfgrasses during a controlled water withdrawal experiment in a greenhouse. Fourteen genotypes from St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntz], Japanese lawngrass (Zoysia japonica Steud.), manillagrass [Zoysia matrella (L.) Merr.], and bermudagrass [Cynodon dactylon (L.) Pers.] were planted in acrylic tubes. Their responses in transpiration, gas exchange rate, and leaf firing to fraction of transpirable soil water were characterized through threshold and midpoint values. Threshold and midpoint variables are promising traits that can be exploited to breed for improved drought responses. Bermudagrass had the lowest threshold for leaf firing compared with other species, indicating that the initiation of leaf firing happened later in the drying cycle. This response may be associated with a lower threshold for relative gas exchange rate. ‘Zeon’ manilagrass exhibited better drought tolerance, which was indicated by its lower midpoint for leaf firing and similar characteristics in transpiration and gas exchange rates compared with ‘Taccoa Green’ manilagrass. Further study is needed to elucidate the underlying mechanism of leaf senescence delay in Zeon. No consistent drought mechanisms were found to be universal among the four warm‐season species, suggesting that there are likely several different mechanisms that may be species dependent.
Utilization of Sunflower Crop Wild Relatives for Cultivated Sunflower Improvement
Authors: Seiler Gerald J. , Qi Lili L. , Marek Laura F.
Published: February 2017

Sunflower (Helianthus annuus L.) is one of the few crops native to the United States. The current USDA–ARS National Plant Germplasm System (NPGS) crop wild relatives sunflower collection is the largest extant collection in the world, containing 2519 accessions comprising 53 species—39 perennial and 14 annual. To fully utilize gene bank collections, however, researchers need more detailed information about the amount and distribution of genetic diversity present within the collection. The wild species are adapted to a wide range of habitats and possess considerable variability for most biotic and abiotic traits. This represents a substantial amount of genetic diversity available for many agronomic traits for cultivated sunflower, which has a very narrow genetic base. Sunflower ranked fifth highest among 13 crops of major importance to global food security surveyed from the mid‐1980s to 2005 in the use of traits from crop wild relatives. The estimated annual economic contribution of the wild species for cultivated sunflower is between US$267 to 384 million. Most of the value is derived from the PET1 cytoplasm from wild H. petiolaris, disease resistance genes, abiotic salt tolerance, and resistance to imidazolinone and sulfonylurea herbicides. Crop wild relatives provide a wide range of valuable attributes for traditional and molecular breeding, as well as for ecological experimentation, and have enabled rapid advances in ecological and evolutionary genetics. The wild species of Helianthus continue to contribute specific traits to combat emerging pests and environmental challenges and, at the same time, are preserved for future generations.
New Algorithm for Detecting Target on the Clutter Background Using Polarimetric Parameter
Authors: Pham Hung Trong , Pham Nghia Minh , Nguyen Thanh Trung
Published: February 2017

This paper proposes a new algorithm for detecting radar targets on the clutter background using a nonenergy polarimetry parameter, the ellipticity coefficient. The algorithm ultilises two-level threshold dectector (is used in this algorithm). Probability density function of ellipticity coefficient is calculated for two classes of target: target in clutter and only clutter. The optimum detection threshold is calculated based on the Neyman-Pearson criteria. In this paper, the detection threshold and the probability of detection are calculated based on a given false alarm, different signal to background clutter ratios, and with different polarimetric features of the background clutter. Proposed algorithm shows the efficiency of using ellipticity coefficient in detecting target on the background clutter.
Baseflow recession characterization and groundwater storage trends in northern Taiwan
Authors: Lin Kun-Ta , Yeh Hsin-Fu
Published: February 2017

Groundwater is a critical component of the terrestrial water budget and acts as a relatively stable water source in Taiwan. In the present study, river basins' characterization and groundwater storage trends in northern Taiwan are analyzed using the Brutsaert method. As groundwater storage sustains baseflows in a water system during dry periods, it can be assessed directly from the streamflow record. The characteristic drainage time scale value, K, varied between 34 and 84 days, with a mean value of 54 days and a standard deviation of 16 days. From correlation analysis, K is strongly correlated with the main channel slope. Based on annual values of groundwater storage over the period of record, five subbasins showed downward trends, ranging from −0.053 to −0.950 mm/year, and three subbasins exhibited upward trends, ranging from 0.111 to 0.141 mm/year. During the period of 2000–2014, the groundwater storage trends in northern Taiwan had an obvious spatial distribution. River basins with significant negative trends (mean value of −2.729 mm/year) are located in the northeast part of the study area. In contrast, the subbasins in the northwest part all showed positive trends (mean value of 0.944 mm/year) in groundwater storage.