bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanohybrid Antifungals for Control of Plant Diseases: Current Status and Future Perspectives
Authors: Alghuthaymi Mousa A. , C. Rajkuberan , P. Rajiv , Kalia Anu , Bhardwaj Kanchan , Bhardwaj Prerna , Abd-Elsalam Kamel A. , Valis Martin , Kuca Kamil
Published: January 2021

The changing climatic conditions have led to the concurrent emergence of virulent microbial pathogens that attack crop plants and exhibit yield and quality deterring impacts on the affected crop. To counteract, the widespread infections of fungal pathogens and post-harvest diseases it is highly warranted to develop sustainable techniques and tools bypassing traditional agriculture practices. Nanotechnology offers a solution to the problems in disease management in a simple lucid way. These technologies are revolutionizing the scientific/industrial sectors. Likewise, in agriculture, the nano-based tools are of great promise particularly for the development of potent formulations ensuring proper delivery of agrochemicals, nutrients, pesticides/insecticides, and even growth regulators for enhanced use efficiency. The development of novel nanocomposites for improved management of fungal diseases can mitigate the emergence of resilient and persistent fungal pathogens and the loss of crop produce due to diseases they cause. Therefore, in this review, we collectively manifest the role of nanocomposites for the management of fungal diseases.
Antifungal Activity of a Medical-Grade Honey Formulation against Candida auris
Authors: de Groot Theun , Janssen Tom , Faro Dirk , Cremers Niels A. J. , Chowdhary Anuradha , Meis Jacques F.
Published: January 2021

Candida auris is a pathogenic yeast causing outbreaks in intensive care units with high mortality rates. The treatment of C. auris colonization is challenging due to high resistance rates. A potential alternative antifungal treatment is medical-grade honey. In this study the susceptibility of C. auris and other Candida species to the medical-grade honey-based formulation L-Mesitran® Soft was investigated. The medical-grade honey formulation reduced the growth of C. auris and other Candida species in a dose-dependent manner. This inhibition was not only due to the honey component, as treatment with an identical concentration of this component only was less effective and even stimulated the growth of C. albicans and C. glabrata, supporting the interpretation that supplements in the medical-grade honey formulation enhanced the antimicrobial activity. Increasing the concentration of the honey component to 40%, as is also present in an undiluted medical-grade honey formulation, lead to a 1- to 4-log inhibition of all Candida species. Unprocessed local honey reduced the growth of nearly all Candida species more strongly than medical-grade honey. C. auris’ susceptibility to the medical-grade honey formulation did not depend on geographic origin or resistance profile, although the multiresistant isolates tended to be more susceptible. Altogether, medical-grade honey formulation has a strong antifungal activity against C. auris and other Candida species. Future studies should demonstrate whether the treatment of open wounds or skin colonized with C. auris is feasible and effective in the clinical setting.
Laboratory-Based Resources for COVID-19 Diagnostics: Traditional Tools and Novel Technologies. A Perspective of Personalized Medicine
Authors: Andryukov Boris G. , Besednova Natalya N. , Kuznetsova Tatyana A. , Fedyanina Ludmila N.
Published: January 2021

The coronavirus infection 2019 (COVID-19) pandemic, caused by the highly contagious SARS-CoV-2 virus, has provoked a global healthcare and economic crisis. The control over the spread of the disease requires an efficient and scalable laboratory-based strategy for testing the population based on multiple platforms to provide rapid and accurate diagnosis. With the onset of the pandemic, the reverse transcription polymerase chain reaction (RT-PCR) method has become a standard diagnostic tool, which has received wide clinical use. In large-scale and repeated examinations, these tests can identify infected patients with COVID-19, with their accuracy, however, dependent on many factors, while the entire process takes up to 6–8 h. Here we also describe a number of serological systems for detecting antibodies against SARS-CoV-2. These are used to assess the level of population immunity in various categories of people, as well as for retrospective diagnosis of asymptomatic and mild COVID-19 in patients. However, the widespread use of traditional diagnostic tools in the context of the rapid spread of COVID-19 is hampered by a number of limitations. Therefore, the sharp increase in the number of patients with COVID-19 necessitates creation of new rapid, inexpensive, sensitive, and specific tests. In this regard, we focus on new laboratory technologies such as loop mediated isothermal amplification (LAMP) and lateral flow immunoassay (LFIA), which have proven to work well in the COVID-19 diagnostics and can become a worthy alternative to traditional laboratory-based diagnostics resources. To cope with the COVID-19 pandemic, the healthcare system requires a combination of various types of laboratory diagnostic testing techniques, whodse sensitivity and specificity increases with the progress in the SARS-CoV-2 research. The testing strategy should be designed in such a way to provide, depending on the timing of examination and the severity of the infection in patients, large-scale and repeated examinations based on the principle: screening–monitoring–control. The search and development of new methods for rapid diagnostics of COVID-19 in laboratory, based on new analytical platforms, is still a highly important and urgent healthcare issue. In the final part of the review, special emphasis is made on the relevance of the concept of personalized medicine to combat the COVID-19 pandemic in the light of the recent studies carried out to identify the causes of variation in individual susceptibility to SARS-CoV-2 and increase the efficiency and cost-effectiveness of treatment.
Evaluation of Soundscapes in Urban Parks in Olsztyn (Poland) for Improvement of Landscape Design and Management
Authors: Jaszczak Agnieszka , Małkowska Natalia , Kristianova Katarina , Bernat Sebastian , Pochodyła Ewelina
Published: January 2021

Soundscape analyses and noise measurements should be a part of pre-design works involved in planning green areas in city centers. The aim of the study was to conduct a multi-criteria analysis of the soundscape of three parks in Olsztyn (Poland) as a part of the landscape planning process to determine the directions of re-design of places most exposed to noise. The research included: 1. functional and spatial analysis of the park surroundings in reference to the city environment, 2. analysis of the acoustic map, 3. measurements of sound pressure levels (SPL) at selected points in two periods (leafless and leafy), 4. analysis of characteristic sounds, 5. interview with park users and preparation of a mental map. The results of research regarding the perception of the soundscape of all three parks by respondents differ slightly from the results of both the acoustic map and SPL measurements. The results also confirm the difference between SPL in the leafless and leafy period. Places most exposed to noise are located at the park boundaries along the main access roads, and at park entrances. Recommendations and sample solutions are proposed, based on two suggested design activities, namely the reduction of undesirable sounds, and introduction of desirable sounds to the parks.
Noncoding RNA: An Insight into Chloroplast and Mitochondrial Gene Expressions
Authors: Anand Asha , Pandi Gopal
Published: January 2021

Regulation of gene expression in any biological system is a complex process with many checkpoints at the transcriptional, post-transcriptional and translational levels. The control mechanism is mediated by various protein factors, secondary metabolites and a newly included regulatory member, i.e., noncoding RNAs (ncRNAs). It is known that ncRNAs modulate the mRNA or protein profiles of the cell depending on the degree of complementary and context of the microenvironment. In plants, ncRNAs are essential for growth and development in normal conditions by controlling various gene expressions and have emerged as a key player to guard plants during adverse conditions. In order to have smooth functioning of the plants under any environmental pressure, two very important DNA-harboring semi-autonomous organelles, namely, chloroplasts and mitochondria, are considered as main players. These organelles conduct the most crucial metabolic pathways that are required to maintain cell homeostasis. Thus, it is imperative to explore and envisage the molecular machineries responsible for gene regulation within the organelles and their coordination with nuclear transcripts. Therefore, the present review mainly focuses on ncRNAs origination and their gene regulation in chloroplasts and plant mitochondria.
Enrichment of Rabbit Primitive Hematopoietic Cells via MACS Depletion of CD45+ Bone Marrow Cells
Authors: Vašíček Jaromír , Baláži Andrej , Bauer Miroslav , Svoradová Andrea , Tirpáková Mária , Ondruška Ľubomír , Parkányi Vladimír , V. Makarevich Alexander , Chrenek Peter
Published: January 2021

Hematopoietic stem and progenitor cells (HSC/HPCs) of human or few animal species have been studied for over 30 years. However, there is no information about rabbit HSC/HPCs, although they might be a valuable animal model for studying human hematopoietic disorders or could serve as genetic resource for the preservation of animal biodiversity. CD34 marker is commonly used to isolate HSC/HPCs. Due to unavailability of specific anti-rabbit CD34 antibodies, a novel strategy for the isolation and enrichment of rabbit HSC/HPCs was used in this study. Briefly, rabbit bone marrow mononuclear cells (BMMCs) were sorted immunomagnetically in order to remove all mature (CD45+) cells. The cells were depleted with overall purity about 60–70% and then cultured in a special medium designed for the expansion of CD34+ cells. Quantitative Polymerase Chain Reaction (qPCR) analysis confirmed the enrichment of primitive hematopoietic cells, as the expression of CD34 and CD49f increased (p < 0.05) and CD45 decreased (p < 0.001) at the end of culture in comparison to fresh BMMCs. However, cell culture still exhibited the presence of CD45+ cells, as identified by flow cytometry. After gating on CD45− cells the MHCI+MHCII−CD38+CD49f+CD90−CD117− phenotype was observed. In conclusion, rabbit HSC/HPCs might be isolated and enriched by the presented method. However, further optimization is still required.
New Mononuclear Mn(III) Complexes with Hydroxyl-Substituted Hexadentate Schiff Base Ligands
Authors: Xu Peng-Yu , Wang Yu-Ting , Yu Zong-Mei , Li Yong-Hua , Wang Shi
Published: January 2021

This paper reports the syntheses, crystal structures and magnetic properties of Mn(III) hexadentate Schiff base complexes [Mn(4-OH-sal-N-1,5,8,12)]NO3(1) and [Mn(4-OH-sal-N-1,5,8,12)]ClO4(2), where (4-OH-sal-N-1,5,8,12)2− (4,4′-((1E,13E)-2,6,9,13-tetraazatetradeca-1,13-diene-1,14-diyl)bis(3-methoxyphenol) is a new hydroxyl-substituted hexadentate Schiff base ligand. The introduction of the (4-OH-sal-N-1,5,8,12)2− ligand induces more hydrogen bonding interactions, in addition to promoting the formation of intermolecular interactions among the cations. However, the close-packing structures of both complexes lead to their stabilization in the high-spin state in the temperature range of 2−300 K.
Microstructural and Mechanical Assessment of Camshafts Produced by Ductile Cast Iron Low Alloyed with Vanadium
Authors: Colin García Eduardo , Cruz Ramírez Alejandro , Reyes Castellanos Guillermo , Téllez Ramírez Jaime , Magaña Hernández Antonio
Published: January 2021

In the present study, ductile iron camshafts low alloyed with 0.2 and 0.3 wt % vanadium were produced to study the microstructural and mechanical evaluation of lobes and camshaft. For this purpose, camshafts were produced in one of the largest manufacturers of the ductile iron camshaft in México by the phenolic urethane no-bake sand mold casting method. The microstructure of the lobes was studied in three zones located at the top, middle, and bottom of the lobes by optical microscopy, and mechanical tests were performed on lobes and camshafts. A homogeneous distribution of spheroidal graphite with high nodularity for both castings was obtained from the regions of the lobes analyzed. The high cooling rate on the lobe surfaces enabled us to obtain a high nodule count of a smaller size instead of the middle region where big nodules with a low nodule count are presented. An inverse chill behavior was found in the middle region of the lobes where there is an increase in the concentration of carbide-forming elements, leading to the highest micro-hardness values in this region. The tensile properties were increased when the vanadium contents were increased; however, the toughness and ductility of the as-cast alloys were decreased as a result of the increase of the volume fraction of carbide particles.
Graphene Coating as an Effective Barrier to Prevent Bacteria-Mediated Dissolution of Gold
Authors: Parra Carolina , Aristizabal Juliet , Arce Bárbara , Montero-Silva Francisco , Lascano Sheila , Henriquez Ricardo , Lazcano Paola , Giraldo-Gallo Paula , Ramírez Cristian , Henrique Rodrigues da Cunha Thiago , Barrera de Brito Angela
Published: January 2021

The interaction of biofilms with metallic surfaces produces two biologically induced degradation processes of materials: microbial induced corrosion and bioleaching. Both phenomena affect most metallic materials, but in the case of noble metals such as gold, which is inert to corrosion, metallophilic bacteria can cause its direct or in direct dissolution. When this process is controlled, it can be used for hydrometallurgical applications, such as the recovery of precious metals from electronic waste. However, the presence of unwanted bioleaching-producing bacteria can be detrimental to metallic materials in specific environments. In this work, we propose the use of single-layer graphene as a protective coating to reduce Au bioleaching by Cupriavidus metallidurans, a strain adapted to metal contaminated environments and capable of dissolving Au. By means of Scanning Tunneling Microscopy, we demonstrate that graphene coatings are an effective barrier to prevent the complex interactions responsible for Au dissolution. This behavior can be understood in terms of graphene pore size, which creates an impermeable barrier that prevents the pass of Au-complexing ligands produced by C.metallidurans through graphene coating. In addition, changes in surface energy and electrostatic interaction are presumably reducing bacterial adhesion to graphene-coated Au surfaces. Our findings provide a novel approach to reduce the deterioration of metallic materials in devices in environments where biofilms have been found to cause unwanted bioleaching.
Development of Coronavirus Treatments Using Neutralizing Antibodies
Authors: Fouladirad Saman , Bach Horacio
Published: January 2021

The Coronavirus disease 2019 (COVID-19), caused by the novel coronavirus SARS-CoV-2, was first reported in December 2019 in Wuhan, Hubei province, China. This virus has led to 61.8 million cases worldwide being reported as of December 1st, 2020. Currently, there are no definite approved therapies endorsed by the World Health Organization for COVID-19, focusing only on supportive care. Treatment centers around symptom management, including oxygen therapy or invasive mechanical ventilation. Immunotherapy has the potential to play a role in the treatment of SARS-CoV-2. Monoclonal antibodies (mAbs), in particular, is a relatively new approach in the world of infectious diseases and has the benefit of overcoming challenges with serum therapy and intravenous immunoglobulins preparations. Here, we reviewed the articles published in PubMed with the purpose of summarizing the currently available evidence for the use of neutralizing antibodies as a potential treatment for coronaviruses. Studies reporting in vivo results were summarized and analyzed. Despite promising data from some studies, none of them progressed to clinical trials. It is expected that neutralizing antibodies might offer an alternative for COVID-19 treatment. Thus, there is a need for randomized trials to understand the potential use of this treatment.
Design of PEGylated Three Ligands Silica Nanoparticles for Multi-Receptor Targeting
Authors: Maurel Manon , Montheil Titouan , Martin Julie , Chaar Line , Guzman-Gonzalez Veronica , Couvet Morgane , Jacquet Thibault , Jia Tao , Eymin Beatrice , Parra Karine , Dumy Pascal , Martinez Jean , Ruggiero Florence , Vaganay Elisabeth , Mehdi Ahmad , Coll Jean-Luc , Subra Gilles
Published: January 2021

The synthesis of silica nanoparticles (SiNPs) decorated on their surface with a range of various elements (e.g., ligands, drugs, fluorophores, vectors, etc.) in a controlled ratio remains a big challenge. We have previously developed an efficient strategy to obtain in one-step, well-defined multifunctional fluorescent SiNPs displaying fluorophores and two peptides ligands as targeting elements, allowing selective detection of cancer cells. In this paper, we demonstrate that additional level of controlled multifunctionality can be achieved, getting even closer to the original concept of “magic bullet”, using solely sol–gel chemistry to achieve conjugation of PEG chains for stealth, along with three different ligands. In addition, we have answered the recurrent question of the surface ungrafting by investigating the stability of different siloxane linkages with the ERETIC Method (Electronic Reference to Access In Vivo Concentrations) by 19F NMR quantification. We also compared the efficiency of the hybrid silylated fluorophore covalent linkage in the core of the SiNP to conventional methods. Finally, the tumor-cell-targeting efficiency of these multi-ligand NPs on human endothelial cells (HUVEC or HDMEC) and mixed spheroids of human melanoma cells and HUVEC displaying different types of receptors were evaluated in vitro.
Influence of N-glycosylation on Expression and Function of Pseudorabies Virus Glycoprotein gB
Authors: Vallbracht Melina , Klupp Barbara G. , Mettenleiter Thomas C.
Published: January 2021

Envelope glycoprotein (g)B is conserved throughout the Herpesviridae and mediates fusion of the viral envelope with cellular membranes for infectious entry and spread. Like all viral envelope fusion proteins, gB is modified by asparagine (N)-linked glycosylation. Glycans can contribute to protein function, intracellular transport, trafficking, structure and immune evasion. gB of the alphaherpesvirus pseudorabies virus (PrV) contains six consensus sites for N-linked glycosylation, but their functional relevance is unknown. Here, we investigated the occupancy and functional relevance of N-glycosylation sites in PrV gB. To this end, all predicted N-glycosylation sites were inactivated either singly or in combination by the introduction of conservative mutations (N➔Q). The resulting proteins were tested for expression, fusion activity in cell–cell fusion assays and complementation of a gB-deficient PrV mutant. Our results indicate that all six sites are indeed modified. However, while glycosylation at most sites was dispensable for gB expression and fusogenicity, inactivation of N154 and N700 affected gB processing by furin cleavage and surface localization. Although all single mutants were functional in cell–cell fusion and viral entry, simultaneous inactivation of all six N-glycosylation sites severely impaired fusion activity and viral entry, suggesting a critical role of N-glycans for maintaining gB structure and function.
Psychophysical Evaluation of the Olfactory Function: European Multicenter Study on 774 COVID-19 Patients
Authors: Vaira Luigi Angelo , Lechien Jerome R. , Khalife Mohamad , Petrocelli Marzia , Hans Stephane , Distinguin Lea , Salzano Giovanni , Cucurullo Marco , Doneddu Piero , Salzano Francesco Antonio , Biglioli Federico , Journe Fabrice , Piana Andrea Fausto , De Riu Giacomo , Saussez Sven
Published: January 2021

Background: The objective evaluation of the olfactory function of coronavirus disease 2019 patients is difficult because of logistical and operator-safety problems. For this reason, in the literature, the data obtained from psychophysical tests are few and based on small case series. Methods: A multicenter, cohort study conducted in seven European hospitals between March 22 and August 20, 2020. The Sniffin-Sticks test and the Connecticut Chemosensory Clinical Research Center orthonasal olfaction test were used to objectively evaluate the olfactory function. Results: This study included 774 patients, of these 481 (62.1%) presented olfactory dysfunction (OD): 280 were hyposmic and 201 were anosmic. There was a significant difference between self-reported anosmia/hyposmia and psychophysical test results (p = 0.006). Patients with gastroesophageal disorders reported a significantly higher probability of presenting hyposmia (OR 1.86; p = 0.015) and anosmia (OR 2.425; p < 0.001). Fever, chest pain, and phlegm significantly increased the likelihood of having hyposmia but not anosmia or an olfactory disturbance. In contrast, patients with dyspnea, dysphonia, and severe-to-critical COVID-19 were significantly more likely to have no anosmia, while these symptoms had no effect on the risk of developing hyposmia or an OD. Conclusions: Psychophysical assessment represents a significantly more accurate assessment tool for olfactory function than patient self-reported clinical outcomes. Olfactory disturbances appear to be largely independent from the epidemiological and clinical characteristics of the patients. The non-association with rhinitis symptoms and the high prevalence as a presenting symptom make olfactory disturbances an important symptom in the differential diagnosis between COVID-19 and common flu.
Plant Health and Sound Vibration: Analyzing Implications of the Microbiome in Grape Wine Leaves
Authors: Wassermann Birgit , Korsten Lise , Berg Gabriele
Published: January 2021

Understanding the plant microbiome is a key for plant health and controlling pathogens. Recent studies have shown that plants are responsive towards natural and synthetic sound vibration (SV) by perception and signal transduction, which resulted in resistance towards plant pathogens. However, whether or not native plant microbiomes respond to SV and the underlying mechanism thereof remains unknown. Within the present study we compared grapevine-associated microbiota that was perpetually exposed to classical music with a non-exposed control group from the same vineyard in Stellenbosch, South Africa. By analyzing the 16S rRNA gene and ITS fragment amplicon libraries we found differences between the core microbiome of SV-exposed leaves and the control group. For several of these different genera, e.g., Bacillus, Kocuria and Sphingomonas, a host-beneficial or pathogen-antagonistic effect has been well studied. Moreover, abundances of taxa identified as potential producers of volatile organic compounds that contribute to sensory characteristics of wines, e.g., Methylobacterium, Sphingomonas, Bacillus and Sporobolomyces roseus, were either increased or even unique within the core music-exposed phyllosphere population. Results show an as yet unexplored avenue for improved plant health and the terroir of wine, which are important for environmentally friendly horticulture and consumer appreciation. Although our findings explain one detail of the long-term positive experience to improve grapevine’s resilience by this unusual but innovative technique, more mechanistic studies are necessary to understand the whole interplay.
Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions
Authors: Stojković Dejan , Drakulić Danijela , Schwirtlich Marija , Rajčević Nemanja , Stevanović Milena , Soković Marina D. , Gašić Uroš
Published: January 2021

Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.
Leading a Digital Transformation in Pharmacy Education with a Pandemic as the Accelerant
Authors: Mirzaian Edith , Franson Kari L.
Published: January 2021

The global COVID-19 pandemic has not only posed a challenge to education but created an opportunity to spearhead a digital transformation and the novel delivery of a Pharm.D. curriculum. The process to transform the curriculum in a sustainable and iterative manner involved multiple steps including: (1) Communication, (2) Maintaining faculty engagement, (3) Allowing outside the box thinking, (4) Providing resources and tools and (5) Creating accountability and timelines. At our institution, we have been interested in digital transformation since completing our interview of global leaders. We began our journey using the current COVID-19 pandemic as an accelerant for change. Digital transformation in any industry is not a simple undertaking. However, with planning, aligned organizational interests, consistent and regular communication, provision of resources and tools, engaging faculty and creating accountability and timelines with deliverables the implementation can be successful. When the global pandemic wanes and educational institutions commence in-person classes, having undergone the stages of digital transformation, we will be able to embrace these changes and transform education, not having to reproduce pre-pandemic educational systems.
Cytinus under the Microscope: Disclosing the Secrets of a Parasitic Plant
Authors: Sanjust Enrico , Rinaldi Andrea C.
Published: January 2021

Well over 1% of all flowering plants are parasites, obtaining all or part of the nutrients they need from other plants. Among this extremely heterogeneous assemblage, the Cytinaceae form a small group of holoparasites, with Cytinus as the main representative genus. Despite the small number of known species and the fact that it doesn’t attack crops or plants of economic importance, Cytinus is paradigmatic among parasitic plants. Recent research has indeed disclosed many aspects of host–parasite interactions and reproductive biology, the latter displaying a vast array of adaptive traits to lure a range of animal pollinators. Furthermore, analysis of biological activities of extracts of the most common species of Cytinus has provided evidence that this plant could be a valuable source of compounds with high potential in key applicative areas, namely food production (nutraceuticals) and the development of antimicrobial therapeutics. This article offers a complete overview of our current knowledge of Cytinus.
Corn Starch (Zea mays) Biopolymer Plastic Reaction in Combination with Sorbitol and Glycerol
Authors: Hazrol M.D. , Sapuan S.M. , Zainudin E.S. , Zuhri M.Y.M. , Abdul Wahab N.I.
Published: January 2021

The research included corn starch (CS) films using sorbitol (S), glycerol (G), and their combination (SG) as plasticizers at 30, 45, and 60 wt %, with a traditional solution casting technique. The introduction of plasticizer to CS film-forming solutions led to solving the fragility and brittleness of CS films. The increased concentration of plasticizers contributed to an improvement in film thickness, weight, and humidity. Conversely, plasticized films reduced their density and water absorption, with increasing plasticizer concentrations. The increase in the amount of the plasticizer from 30 to 60% showed a lower impact on the moisture content and water absorption of S-plasticized films. The S30-plasticized films also showed outstanding mechanical properties with 13.62 MPa and 495.97 MPa, for tensile stress and tensile modulus, respectively. Glycerol and-sorbitol/glycerol plasticizer (G and SG) films showed higher moisture content and water absorption relative to S-plasticized films. This study has shown that the amount and type of plasticizers significantly affect the appearances, physical, morphological, and mechanical properties of the corn starch biopolymer plastic.
Nanocellulose/Fullerene Hybrid Films Assembled at the Air/Water Interface as Promising Functional Materials for Photo-electrocatalysis
Authors: Milano Francesco , Guascito Maria Rachele , Semeraro Paola , Sawalha Shadi , Da Ros Tatiana , Operamolla Alessandra , Giotta Livia , Prato Maurizio , Valli Ludovico
Published: January 2021

Cellulose nanomaterials have been widely investigated in the last decade, unveiling attractive properties for emerging applications. The ability of sulfated cellulose nanocrystals (CNCs) to guide the supramolecular organization of amphiphilic fullerene derivatives at the air/water interface has been recently highlighted. Here, we further investigated the assembly of Langmuir hybrid films that are based on the electrostatic interaction between cationic fulleropyrrolidines deposited at the air/water interface and anionic CNCs dispersed in the subphase, assessing the influence of additional negatively charged species that are dissolved in the water phase. By means of isotherm acquisition and spectroscopic measurements, we demonstrated that a tetra-sulfonated porphyrin, which was introduced in the subphase as anionic competitor, strongly inhibited the binding of CNCs to the floating fullerene layer. Nevertheless, despite the strong inhibition by anionic molecules, the mutual interaction between fulleropyrrolidines at the interface and the CNCs led to the assembly of robust hybrid films, which could be efficiently transferred onto solid substrates. Interestingly, ITO-electrodes that were modified with five-layer hybrid films exhibited enhanced electrical capacitance and produced anodic photocurrents at 0.4 V vs Ag/AgCl, whose intensity (230 nA/cm2) proved to be four times higher than the one that was observed with the sole fullerene derivative (60 nA/cm2).
Noncatalytic Biodiesel Synthesis under Supercritical Conditions
Authors: Makareviciene Violeta , Sendzikiene Egle
Published: January 2021

The constant increase in greenhouse gases in the environment is forcing people to look for different ways to reduce such pollution. One of these ways is the use of biodiesel for road transport. Conventional biodiesel production involves the catalytic triglyceride transesterification process. When using homogeneous two-stage catalysis, it is difficult to purify the resulting product from the by-products formed, and the catalysts cannot be reused. In the case of heterogeneous catalysis, the process costs are increased due to separation and regeneration of the catalysts. To solve these problems of catalytic synthesis, a noncatalytic process has been recently studied that which takes place under supercritical conditions for an alcohol or other acyl receptor. In such biodiesel production, fatty feedstocks and alcohols are used as raw materials, with the synthesis taking place at supercritical conditions for alcohol, i.e., high temperature and pressure, thus making the process quite simple. This paper reviews the results obtained from biodiesel synthesis using a noncatalytic supercritical process for transesterification using both alcohols and carboxylate esters of low molecular weight, evaluating the optimal conditions for these processes and biofuel stability at high temperatures.
Iterative Method for Tuning Multiloop PID Controllers Based on Single Loop Robustness Specifications in the Frequency Domain
Authors: Garrido Juan , Ruz Mario , Morilla Fernando , Vázquez Francisco
Published: January 2021

Multiloop proportional-integral-derivative (PID) controllers are widely used for controlling multivariable processes due to their understandability, simplicity and other practical advantages. The main difficulty of the methodologies using this approach is the fact that the controllers of different loops interact each other. Thus, the knowledge of the controllers in the other loops is necessary for the evaluation of one loop. This work proposes an iterative design methodology of multiloop PID controllers for stable multivariable systems. The controllers in each step are tuned using single-input single-output (SISO) methods for the corresponding effective open loop process (EOP), which considers the interaction of the other loops closed with the controllers of the previous step. The methodology uses a frequency response matrix representation of the system to avoid process approximations in the case of elements with time delays or complicated EOPs. Consequently, different robustness margins on the frequency domain are proposed as specifications: phase margin, gain margin, phase and gain margin combination, sensitivity margin and linear margin. For each case, a PID tuning method is described and detailed for the iterative methodology. The proposals are exemplified with two simulations systems where the obtained performance is similar or better than that achieved by other authors.
Optimal Investment in Cyber-Security under Cyber Insurance for a Multi-Branch Firm
Authors: Mazzoccoli Alessandro , Naldi Maurizio
Published: January 2021

Investments in security and cyber-insurance are two cyber-risk management strategies that can be employed together to optimize the overall security expense. In this paper, we provide a closed form for the optimal investment under a full set of insurance liability scenarios (full liability, limited liability, and limited liability with deductibles) when we consider a multi-branch firm with correlated vulnerability. The insurance component results to be the major expense. It ends up being the only recommended approach (i.e., setting zero investments in security) when the intrinsic vulnerability is either very low or very high. We also study the robustness of the investment choices when our knowledge of vulnerability and correlation is uncertain, concluding that the uncertainty induced on investment by either uncertain correlation or uncertain vulnerability is not significant.
Analyzing Urban Spatial Patterns and Functional Zones Using Sina Weibo POI Data: A Case Study of Beijing
Authors: Miao Ruomu , Wang Yuxia , Li Shuang
Published: January 2021

With the development of Web2.0 and mobile Internet, urban residents, a new type of “sensor”, provide us with massive amounts of volunteered geographic information (VGI). Quantifying the spatial patterns of VGI plays an increasingly important role in the understanding and development of urban spatial functions. Using VGI and social media activity data, this article developed a method to automatically extract and identify urban spatial patterns and functional zones. The method is put forward based on the case of Beijing, China, and includes the following three steps: (1) Obtain multi-source urban spatial data, such as Weibo data (equivalent to Twitter in Chinese), OpenStreetMap, population data, etc.; (2) Use the hierarchical clustering algorithm, term frequency-inverse document frequency (TF-IDF) method, and improved k-means clustering algorithms to identify functional zones; (3) Compare the identified results with the actual urban land uses and verify its accuracy. The experiment results proved that our method can effectively identify urban functional zones, and the results provide new ideas for the study of urban spatial patterns and have great significance in optimizing urban spatial planning.
The Innovative Model of Runway Sustainable Management on Smaller Regional Airports
Authors: Kovačič Boštjan , Doler Damjan , Sever Drago
Published: January 2021

Airport runways are exposed to large traffic loads and other external factors which cause their transformation. Monitoring the state of the unevenness and determining the runway defectiveness is a complex procedure which includes fast and qualitative measurements of the execution of flatness, their evaluation and the determination of the level of runway defectiveness. For this purpose, an improved process of determining airport runway defectiveness is proposed, based on geodetic survey and the management information system prototype, which enables evaluating the results of the executed survey and suggesting the runway maintenance measures. A similar analysis of the considered data on the example of a smaller, regional airport in Maribor, Slovenia, was performed with the help of our own algorithm, which, on various platforms, enables the tabular and graphical display of surface irregularities. The algorithm prototype allows the automated monitoring and evaluating of the unevenness or runway states, determining their defectiveness and advising adequate action. With the help of the suggested method and developed information system prototypes, it is possible to confirm the demand for professionally adequate and on-time planning of necessary maintenance works, with which it is surely possible to prevent an eventual unexpected closing of the runway and related costs. The suggested method is also usable in evaluating the state of other traffic infrastructure.
Matching Demolition and Construction Material Flows, an Urban Mining Case Study
Authors: Verhagen Teun Johannes , Sauer Marijn Louise , van der Voet Ester , Sprecher Benjamin
Published: January 2021

The recycling of demolition waste is essential to lower the construction sectors primary material demand, responsible for 50% of the global primary material consumption. Almost all demolition waste is used as filler material for the construction of roads, preventing further reuse or recycling after this application. The built environment generates considerable annual material in-and outflows. However, there has been little discussion on the availability and further application of this potential supply of secondary materials as a replacement for primary materials. In this study, we quantify the percentage of demolition waste that can be repurposed as secondary materials in the Dutch construction sector. We analyzed the yearly building material flows for the municipality of Leiden using municipal data on demolition and construction to explore the viability of the Dutch government’s policy goal to reduce primary materials consumption by 50% before 2030. From this analysis, we find that the recycling of demolition waste has a sizable potential but just falls short of the stated policy goal. Even in a situation with more construction than demolition, there will remain a considerable mismatch in the yearly construction material demand and available supply of demolition waste for our municipal-wide case study. More importantly, the current processing of demolition waste in the Netherlands will require significant improvements to achieve this goal. New governmental policies are required to focus on maintaining material quality and allowing further use of recycled materials as buildings materials.
Towards an Inclusive Museum Management Strategy. An Exploratory Study of Consumption Experience in Visitors with Disabilities. The Case of the CosmoCaixa Science Museum
Authors: Cerdan Chiscano Monica , Jiménez-Zarco Ana Isabel
Published: January 2021

In the field of cultural tourism, museums have been pioneers in focusing on visitors with disabilities. They have used inclusive orientation to offer the kind of memorable and satisfying experiences that are so beneficial to a group at constant risk of social exclusion. Their task is made more difficult, however, by visitors’ perception of barriers to inclusion, and identification therefore becomes a priority at the strategic level. Based on this idea, the present study analyzes experiences from two tours of the CosmoCaixa Barcelona museum by 32 people with disabilities (PwD). The use of ethnographic techniques and post-experience interviews shows how, despite the legal framework in relation to people with disabilities, hospitality managers find it difficult to put this framework into practice and cater to the needs of this segment of the population. Two factors become particularly clear: the difficulties experienced by museum staff in identifying people with disability, and museum managers’ lack of training and knowledge of the wants and needs of people with disabilities. Academic and strategic recommendations for museum managers are provided at the end of the article.
Knowledge Integration in the Politics and Policy of Rapid Transitions to Net Zero Carbon: A Typology and Mapping Method for Climate Actors in the UK
Authors: Smith Steven R. , Christie Ian
Published: January 2021

The types of political and policy knowledge required to guide rapid transition to low-carbon economies remain largely disconnected in the fields of political science, psychology, and sociology. The composition and key features of the main actors involved, and their relationships have also not been systematically described. This paper attempts to address these knowledge gaps by proposing a new, integrative typology of actors involved in climate change mitigation policy in the United Kingdom (UK) and a method for mapping these actors and selecting their typological descriptors onto a 2D space. The mapping method enables stakeholders to visualise and evaluate the strength of support for the UK government’s net-zero-by-2050 “green growth” strategy and the tensions, challenges, and strategic opportunities potentially facing more radical alternatives. The methodology could be replicated for climate actors in other countries and, in principle, for any geographical scale or level of governance.
Impact on Myanmar’s Logistics Flow of the East–West and Southern Corridor Development of the Greater Mekong Subregion—A Global Logistics Intermodal Network Simulation
Authors: Yamaguchi Takuya , Shibasaki Ryuichi , Samizo Hiroyuki , Ushirooka Hisanari
Published: January 2021

This study focuses on container shipping in Myanmar, which is expected to grow manifold in the near future, given the country’s rapid economic growth rates. This study simulates the impact of Myanmar’s logistics policies on container shipping. These initiatives include the improvement of the East–West Corridor of the Greater Mekong Subregion and the development of the Southern Corridor and Dawei port. The global logistics intermodal network simulation model including both maritime shipping and land transport, is applied to the land-based southeast Asia (ASEAN) region. The estimated results obtained for several different scenarios are crosschecked and compared with the available information on observed flows. Based on the simulation results, the authors conclude that policies that reduce cross-border barriers and improve service levels in Dawei port would result in Thailand using Myanmar’s ports for their cargo as well.
Analysis of Non-Structural Carbohydrate in Relation with Shoot Elongation of Rice under Complete Submergence
Authors: Nurrahma Arinal Haq Izzawati , Yabuta Shin , Junaedi Ahmad , Sakagami Jun-Ichi
Published: January 2021

Regulation of non-structural carbohydrates (NSCs) are important for plants in response to submergence. In this study, the difference in non-structural carbohydrates in relation with shoot elongation between Sub1A and non-Sub1A rice genotypes was investigated. Two rice genotypes, namely Inpari30 (Sub1A genotype) and IR72442 (non-Sub1A genotype), were submerged completely for 6 days and re-aerated by lowering water level up to stem base for 6 days of post submergence. In addition, non-submerged plants (control) was treated with water level up to stem base during the experiment. Photosynthesis rate decreased in both submerged Inpari30 and IR72442 genotypes 71% and 96% lower than their control, respectively. Submerged IR72442 declined Fv/Fm 15.6% lowest than its control and both control and submerged Inpari30. Investigation of the distribution of starch and soluble sugar content in plant organs suggested that shoot elongation of non-Sub1A genotype led to starch and sugar consumption that distributed faster to the new developed organ during submergence. In contrast, Sub1A genotype of Inpari30, which did not exhibit shoot elongation and showed slower NSCs distribution during submergence, performed better on post submergence by maintaining NSCs and distributing to the new developed organ faster than IR72442. These results suggest that Sub1A genotype managed elongation and NSCs during submergence more efficiently than non-Sub1A genotype.
Proposing Alternative Solutions to Enhance Natural Ventilation Rates in Residential Buildings in the Cfa Climate Zone of Rasht
Authors: Aeinehvand Roya , Darvish Amiraslan , Baghaei Daemei Abdollah , Barati Shima , Jamali Asma , Malekpour Ravasjan Vahid
Published: January 2021

Today, renewable resources and the crucial role of passive strategies in energy efficiency in the building sector toward the sustainable development goals are more indispensable than ever. Natural ventilation has traditionally been considered as one of the most fundamental techniques to decrease energy usage by building dwellers and designers. The main purpose of the present study is to enhance the natural ventilation rates in an existing six-story residential building situated in the humid climate of Rasht during the summertime. On this basis, two types of ventilation systems, the Double-Skin Facade Twin Face System (DSF-TFS) and Single-Sided Wind Tower (SSWT), were simulated through DesignBuilder version 4.5. Then, two types of additional ventilation systems were proposed in order to accelerate the airflow, including four-sided as well as multi-opening wind towers. The wind foldable directions were at about 45 degrees (northwest to southeast). The simulation results show that SSWT could have a better performance than the aforementioned systems by about 38%. Therefore, the multi-opening system was able to enhance the ventilation rate by approximately 10% during the summertime.