bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of the Transmutation Operator Method to the Solution of an Initial Boundary Value Problem for the Equation of Multidimensional Free Transverse Vibration of a Thin Elastic Plate Containing the Bessel Operator
Authors: Karimov Shakhobiddin , Tulasheva Yorkinoy
Published: June 2024

In this paper, the transmutation operator method is used to construct an exact solution to the initial boundary value problem for the equation of multidimensional free transverse vibration of a thin elastic plate with a singular Bessel operator acting on geometric variables. We emphasize that multidimensional Erdélyi–Kober operators of fractional order have the property of a transmutation operator, allowing one to transform more complex multidimensional partial differential equations with singular coefficients acting over all variables into simpler ones. If formulas for solutions are known for a simple equation, then we also obtain representations for solutions to the first complex partial differential equation with singular coefficients. In particular, it is successfully applied to the singular differential equations, especially with Bessel-type operators. Using this operator, the considered problem is reduced to a similar problem without the Bessel operator. Based on the solution to the auxiliary problem, an exact solution to the original problem is constructed and analyzed.
Indolizidines from Actinomycetes: An Overview over Producers, Biosynthesis and Bioactivities
Authors: Krause Janina
Published: June 2024

Indolizidines have long been recognized for their valuable bioactivities, their common feature being a bicyclic structure connected via a nitrogen atom. Traditionally, plants have been identified as the primary producers. However, recent discoveries have revealed that certain bacterial strains belonging to the genus of actinomycetes also possess the ability to synthesize various indolizidine-based compounds. Among these strains, Streptomyces sp. HNA39, Saccharopolyspora sp. RL78, and Streptomyces NCIB 11649 have been identified as producers of cyclizidines, characterized by their distinctive cyclopropyl moiety. Additionally, Streptomyces griseus OS-3601 synthesizes a unique class of indolizidine derivatives known as iminimycines, distinguished by their rare imine-cation structure. Protoplast fusion of a Streptomyces griseus strain with Streptomyces tenjimariensis resulted in a new indolizidine named in-dolizomycin. This review aims to provide an overview of known bacterial indolizidine producers, summarize current knowledge regarding the biosynthesis of cyclizidines and iminimycines, and assess their bioactivities.
YOLOv10 to Its Genesis: A Decadal and Comprehensive Review of The You Only Look Once Series
Authors: Sapkota Ranjan , Qureshi Rizwan , Flores-Calero Marco , Hussain Muhammad , Badjugar Chetan , Nepal Upesh , Poulose Alwin , Zeno Peter , Bhanu Prakash Vaddevolu Uday , Yan Hong , Karkee Manoj
Published: June 2024

This review systematically examines the progression of the You Only Look Once (YOLO) object detection algorithms from YOLOv1 to the recently unveiled YOLOv10. Employing a reverse chronological analysis, this study examines the advancements introduced by YOLO algorithms, beginning with YOLOv10 and progressing through YOLOv9, YOLOv8, and subsequent versions to explore each version's contributions to enhancing speed, accuracy, and computational efficiency in real-time object detection. The study highlights the transformative impact of YOLO across five critical application areas: automotive safety, healthcare, industrial manufacturing, surveillance, and agriculture. By detailing the incremental technological advancements that each iteration brought, this review not only chronicles the evolution of YOLO but also discusses the challenges and limitations observed in each earlier versions. The evolution signifies a path towards integrating YOLO with multimodal, context-aware, and General Artificial Intelligence (AGI) systems for the next YOLO decade, promising significant implications for future developments in AI-driven applications.
Emergent Combining Factual Penile Girth Enhancement and Erection Restoration Based on Revolutionary Penile Fibro-vascular Assembly
Authors: Chang Ko-Shih , Chung Cho-Hsing , Chang Yi-Kai , Hsu Geng-Long , Tsai Mang-Hung , Chueh Jeff SC
Published: June 2024

ABSTRACT
Introduction: Traditional penile venous surgery and enhancement have been controversial. Based on De Novo penile fibrovascular assembly, we report on penile venous stripping (PVS) and factual penile girth enhancement (FPGE). Methods: From 2013 to 2023, 18 men underwent PVS and FPGE, all confirmed with veno-occlusive dysfunction (VOD). PVS involves the stripping of erection-related veins. FPGE was performed along the tunica albuginea bilaterally; then, the two tunic defects were repaired with a 70.0x30.0 mm venous stripe and covered with either autologous venous walls (AVW, n=10) or Surgiform (SF, n=8). The penile girth was measured, and radio-opaque contrast was used. The abridged 5-item version of the International Index of Erectile Function (IIEF-5) score and the Erection Hardness Scale (EHS) were used yearly during follow-up via the Internet. Results: The follow-up period was 5.3±1.5 years. Radiopacity was consistently enhanced. Although there was indifference between AVW and SF, there was a significant improvement (both P＜0.01) in IIEF-5 and EHS scores (9.7±2.8 vs. 20.8±2.3; 1.7±0.6 vs. 3.2±0.2, respectively). Both the diameters of the glans and penile shaft increased (28.0±2.3mm and 28.3±2.1mm vs 35.3±2.2mm and 36.3±2.1mm respectively). The satisfaction rate was 81.3% (13/16). Conclusions: This novel combination of PVS and FPGE is promising, although a larger sample size is needed for further validation.
Implementation of Online Path Planning and Obstacle Avoidance Using Yolo for Autonomous Mobile Robots
Authors: Anand Ajay , Suresh Devika , AJ Megha , S Praseetha , Zaheer Shyba
Published: June 2024

Robotic path planning and navigation algorithms enable autonomous mobile robots to navigate complex environments. These algorithms allow robots to be deployed in search and rescue missions, where they can locate victims in hazardous areas inaccessible to humans. Additionally, autonomous driving algorithms hold the potential to revolutionize transportation by enabling self-driving cars to navigate roads safely and efficiently. This paper focuses on developing an algorithm for autonomous navigation of the mobile robot (Tortoise bot) using robot operating system (ROS), based on publish-subscribe communication facilitating seamless interaction between various nodes. Tortoise Bot is a differential wheeled robot whose movement is based on two separately driven wheels placed on either side of the robot body. The Raspberry Pi acts as the controller. A 360-degree LiDAR and RGB-D camera are used for creating a 3D map of the environment. Various state-of-the art software tools, such as Gazebo, Ubuntu, MATLAB and RPI imager are used. Initially, a simulation based on simple obstacle avoidance is done using gazebo and MATLAB. Another simulation based on trajectory tracking is implemented using MATLAB to study the kinematic model. Online navigation and path planning are executed with the help of teleoperation and Dynamic Window Approach algorithm. A novel Wander algorithm is created that avoids obstacles on its path. Object recognition is also implemented during autonomous navigation using YOLO.
K-means++ for Critical Component Identification: Power Grid Case Study Using Measurement-Based Analysis
Authors: SaeedKandezy Reza , Jiang John
Published: June 2024

The inherent capabilities of the K-means++ algorithm to approximate system dynamics within complex systems are subjected by constructing a network structure that captures interconnections among identified components, extending its original purpose as a data clustering method and transforming it into a tool for systems analysis. Leveraging advanced measurement technologies and sophisticated data collection systems, the K-means++ algorithm unveils hidden relationships among components and identifies critical elements.
This study explored the algorithm's potential, facilitating the identification of critical components to enhance system operation, control, optimization, and decision-making and examining the practicality and resiliency of the method in real-world application with noisy and limited data. A case study conducted on power systems (IEEE 39-bus and IEEE 300-bus systems) exemplifies K-means++'s capacity to accurately identify critical components and approximate system dynamics, supported by performance metrics affirming its effectiveness and robustness in system analysis through measurements of bus similarity within clusters based on standard deviation and comparison of net tie-line flows in equivalent networks with the original network across scenarios.
Performance metrics, including the Silhouette Score, Davies-Bouldin Index, and Variation of Information (VI) score, further validated K-means++'s performance, yielding reliable and consistent results.
A Brief Report on the Caregiver Burden: Exploring the Risk and Psychological Factors in Caregivers of Patients with Parkinson’s Disease
Authors: Carpinelli Luna , Savarese Giulia , Luisi Ettore , Pellegrino Francesca , Pellecchia Maria Teresa
Published: June 2024

Background: Parkinson's disease (PD) is a neurodegenerative disorder that affects both patients and their caregivers, particularly parents who often become informal caregivers. Caring for someone with PD is linked to increased psychiatric morbidity and persistent anxiety-depressive distress. Methods: This study investigates the burden on caregivers of PD patients using multi-dimensional scales and identifies the personological and environmental factors contributing to this burden. Results: 20.6% of participants reported a severe burden according to the Caregiver Burden Inventory (CBI), while 13.8% had a moderate to severe burden according to the Zarit Caregiver Burden Inventory (ZBI). The Family Strain Questionnaire Short Form (FSQ-SF) high-lighted a minority of caregivers needing psychological support, with some cases requiring ur-gent attention, particularly in stress-related symptoms (SR) and urgent support (U). The Depres-sion Anxiety Stress Scales Short Version (DASS-21) results showed a higher prevalence of stress-related symptoms compared to anxiety and depression. Retired caregivers reported higher ZBI scores and lower life satisfaction levels compared to working caregivers. Additionally, years of schooling negatively correlated with the ZBI score. T-test analysis indicated that caregivers of patients with cognitive impairment were at greater risk compared to caregivers of patients with-out cognitive impairment. Conclusions: The results underscore the need for early recognition of risky situations and adequate support for caregivers.
Validity and Reliability of the GAITWell®: A Low-Cost Modular System for Gait Analysis
Authors: Dias Wellingtânia Domingos , Kirkwood Renata , Brito Iury Cardoso , Capanema Ivo Oliveira , Sesselmann Meinhard , Vimieiro Claysson Bruno Santos , Huebner Rudolf
Published: June 2024

Background: Gait analysis systems offer invaluable insights for rehabilitation, yet their expense limits clinician access. We developed GAITWell®, a low-cost modular system for capturing spatiotemporal gait variables, and evaluated its measurement properties. Methods: The GAITWell® uses discreet binary sensors on interconnected boards to collect gait data, which is then analyzed using the DBSCAN algorithm on the Cartesian points from sensor outputs. Reliability was assessed using ICC2,1, standard error of the mean (SEM) and Bland-Altman plots. Validity was determined by comparing GAITWell® with the Qualisys Pro-Reflex system. Results: Participated 38 healthy adults, with an average age of 33.2 years (SD 13.0). Correlations between GAITWell® and the Qualisys system ranged from moderate to very high for most gait variables, with the exception of stride length, which demonstrated a low but significant correlation (r = .360, p < .05). The ICC2,1 indicated moderate to good agreement for most gait variables. Stance and double support times, cadence, and base of support exhibited poor reliability, characterized by larger SEM and limits of agreement. Conclusions: Our findings indicate that the GAITWell® system is a promising tool for gait analysis. Future research will enhance sensor accuracy and refine algorithms to improve reliability.
Immunotherapeutic Potential of Mutated NPM1 for the Treatment of AML
Authors: Greiner Jochen , Mohamed Eithar , Fletcher Daniel M , Schuler Patrick J. , Schrezenmeier Hubert , Götz Marlies , Guinn Barbara-ann
Published: June 2024

Acute myeloid leukemia (AML) is a malignant disease of the blood and bone marrow that is characterized by uncontrolled clonal proliferation of abnormal myeloid progenitor cells. Nucleophosmin 1 (NPM1) gene mutations are the most common genetic abnormality in AML, detectable in blast cells from about one third of adults with AML. AML NPM1mut is recognized as a separate entity in the World Health Organization (WHO) classification of AML. Clinical and survival data suggest that patients with this form of AML often have a more favorable prognosis, which may be due to the immunogenicity created by the mutations in the NPM1 protein. Consequently, AML with NPM1mut can be considered an immunogenic subtype of AML. However, the underlying mechanisms of this immunogenicity and associated favorable survival outcomes need to be further investigated. Immune checkpoint molecules, such as the programmed cell death-1 (PD-1) protein and its ligand, PD-L1, play important roles in leukemogenesis through their maintenance of an immunosuppressive tumor microenvironment. Preclinical trials have shown that the use of PD-1/PD-L1 (PDx) checkpoint inhibitors in solid tumors and lymphoma work best in novel therapy combinations. Patients with NPM1mut AML may be better suited to immunogenic strategies that are based on the inhibition of the immune checkpoint pathway of PDx than patients without this mutation, suggesting the genetic landscape of patients may also inform best practice for the use of PDx inhibitors.
Hyperglycemia Stimulates the Irreversible Catabolism of Branched-Chain Amino Acids and Generation of Ketone Bodies by Cultured Human Astrocytes
Authors: Gondas Eduard , Baranovicova Eva , Šofranko Jakub , Murin Radovan
Published: June 2024

Astrocytes are considered to possess a noticeable role in brain metabolism and as a partners in neuron-glia cooperation contribute to synthesis, bioconversions and regulation of the flux of substrates for neuronal metabolism. With the aim to investigate to which extend are human astrocytes metabolizing amino acids and by which compounds are they enriching their surrounding we employed the metabolomics analysis of their culture media by 1H-NMR. In addition, we compered the composition of media with either 5 mM or 25 mM glucose. The quantitative analysis of culture media by 1H-NMR revealed that astrocytes readely dispose from their milieu glutamine, branched-chain amino acids, and pyruvate with significantly high rate, while they enrich the culture media with lactate, branched-chain keto acids, citrate, acetate, ketone bodies, and alanine. The hyperglycemia suppressed the capacity of astrocytes to release branched-chain amino acids while stimulating the generation of ketone bodies. Our results highlight the active involvement of astrocytes in metabolism of several amino acids, and the regulation of key metabolic intermediates. The observed metabolic activities of astrocytes provide valuable insights into their roles in supporting neuronal function, brain metabolism, and intercellular metabolic interactions within the brain. Understanding the complex metabolic interactions between astrocytes and neurons is essential for elucidating brain homeostasis and the pathophysiology of neurological disorders.
Molecular Characterization and Genomic Surveillance of SARS-CoV-2 Lineages in Central India
Authors: Dwivedi Purna , Sharma Mukul , Ansari Afzal , Ghosh Arup , Bishwal Subasa Chandra , Ray Suman Kumar , Katiyar Manish , Subbiah Kombiah , Sahare Lalit , Ukey Mahendra , Barde Pradip , Das Aparup , Singh Pushpendra
Published: June 2024

Since the first reported case of COVID-19 in December 2019, several SARS-CoV-2 variants has evolved and some of them have shown higher transmissibility becoming the prevalent strains. Genomic epidemiological investigations into strains from different time points including the early stages of the pandemic are very crucial for understanding the evolution and transmission patterns. Using whole genome sequences, our study describes the early landscape of SARS-CoV-2 variants in central India retrospectively (including the first known occurrence of SARS-CoV-2 in Madhya Pradesh). We performed amplicon based whole genome sequencing of randomly selected SARS-CoV-2 isolates (n=38) collected between 2020-2022 at state level VRDL, ICMR-NIRTH, Jabalpur from 11899 RT-qPCR positive samples. We observed the presence of five lineages namely B.1, B.1.1, B.1.36.8, B.1.195 and B.6 in 19 genomes from the first wave cases and variants of concern (VOC) lineages i.e. B.1.617.2 (Delta), and BA.2.10 (Omicron) in the second wave cases. There was a shift in mutational pattern of SRAS-CoV-2 strains from the second wave in contrast to the first wave. We have identified five immune escape variants in the S gene: P681R, P681H, L452R, Q57H, and N501Y in the isolates collected during the second wave. Furthermore, these genomes were compared with 2160 complete genome sequences reported from central India that encompass 109 different SARS-CoV-2 lineages. Among them, VOC lineages Delta (28.93%), and Omicron (56.11%) were circulating predominantly in this region. This study provides useful insights into the genetic diversity of SARS-CoV-2 strains over the initial course of the COVID-19 pandemic in central India.
Herpes Simplex Virus Type 1 Infection of Human Periodontal Ligament.
Authors: Chevalier Marlène , Ortis Morgane , Olivieri Charles-Vivien , Vitale Sébastien , Paul Adrien , Tonoyan Lilit , Doglio Alain , Marsault Robert
Published: June 2024

The periodontal ligament (PDL) is a complex connective tissue that connects the tooth root to the dental alveolar bone and plays crucial mechanical roles. PDL also exhibits regenerative roles and regulatory functions to maintain per-iodontium integrity and homeostasis. While PDL exposure to oral microbial pathogens is common, virtually nothing is known regarding viral infections of PDL. In particular, human herpes simplex virus type 1 (HSV-1) persistently infects the oral cavity through infections of the oral epithelium, connective tis-sue and neurons. While the oral spread of HSV-1 is generally asymptomatic, this virus has also been implicated in various oral pathologies. In this study, using a primary cell model derived from PDL (PDL-cells), and whole surgical fragments of PDL, we provide evidence supporting the efficient infection of PDL by HSV-1 and the promotions of cytopathic effects. Infection of PDL by HSV-1 was also associated with an acute innate inflammatory response, as il-lustrated by the production of the antiviral interferons and pro-inflammatory cytokines. Furthermore, this inflammatory response to HSV-1 was exacerbated in the presence of bacterial-derived products, such as peptidoglycans. This work therefore highlights the ability of HSV-1 to infect mesenchymal cells from PDL, suggesting that PDL may serve as a viral reservoir for the perio-dontal spread of HSV-1. Moreover, this raises questions about HSV-1 oral pathogenesis, as HSV-1-associated cytopathic and inflammatory effects may contribute to profound alterations of PDL integrity and functioning.
Internal Structure of Dietary Habits as a Restriction on Healthy Eating Policy in Japan
Authors: Hazama Makoto , Satoh Kouji , Maeda-Yamamoto Mari , Nishihira Jun
Published: June 2024

Although promoting healthy eating is a policy objective, the controllability of dietary habits remains uncertain. The personal dietary patterns reflect many factors, of which some are relatively controllable for individual, and others are not. In this article, assuming that some sort of information about controllability of dietary habits is contained in the observed pattern of food consumptions, we focused on dietary pattern on its own. We introduced a statistical descriptive model for data on food frequency questionnaire, estimated strength of pairwise linkage between foodstuffs, and grouped foodstuffs by applying community detection to the networks of the estimated inter-food linkages. Those linkages represent co-movement of pairs of foods consumption. Furthermore, we demonstrated an analysis on the relationship between health of mind and dietary habits considering the aspect of controllability of dietary habits. Using an observational study in Japan, we obtained the following results: 115 foodstuffs were divided into 3 groups for both of genders, but the compositions were different by gender; in the analysis of mental health, some stress response items were associated with the dependence on some of those food groupings. As the grouping of foodstuffs based on our estimation depicted the internal structure of dietary habit that a healthy eating policy should regard as constraint, it follows that we should design the policy along the line with that grouping.
Study Protocol for a Prospective Self-Controlled Trial on Success in Meeting Comprehensive Genomic Profiling Analysis Criteria for Specimens Obtained by Endoscopic Ultrasound-Guided Tissue Acquisition Using a 19G Needle from Primary and Metastatic Lesions
Authors: Takeshita Kotaro , Hijioka Susumu , Nagashio Yoshikuni , Hara Hidenobu , Agarie Daiki , Kawasaki Yuki , Takasaki Tetsuro , Yagi Shin , Hagiwara Yuya , Okamoto Kohei , Yamashige Daiki , Fukuda Soma , Kuwada Masaru , Komori Yasuhiro , Okada Mao , Maruki Yuta , Morizane Chigusa , Ueno Hideki , Yatabe Yasushi , Okusaka Takuji
Published: June 2024

EUS–TA in unresectable pancreatic cancer requires not only a tissue diagnosis but also tissue collection in anticipation of comprehensive genomic profiling. However, the optimal puncture target remains controversial. Therefore, the Primary and Metastatic Lesions in Pancreatic Cancer (PRIMATE) study was designed to clarify the optimal target by comparing the success rates for meeting OncoGuide NCC Oncopanel (NOP) analysis criteria on pre-check primary and metastatic lesion specimens obtained during the same EUS–TA session in patients with invasive pancreatic ductal adenocarcinoma. In this ongoing prospective study, two specimens, each from primary and metastatic lesions, are obtained by EUS–TA (typically using a 19G fine-needle biopsy needle) in patients with invasive pancreatic ductal adenocarcinoma. The primary endpoint is the proportion of EUS–TA specimens that meet NOP analysis criteria during pre-check (i.e., tumor cellularity ≥ 20% and tissue area ≥ 4 mm2), which are then compared between primary and metastatic lesions. This study has been approved by the National Cancer Center Institutional Review Board (Research No. 2022-168). The results of this study will be reported at an international conference and published in an international peer-reviewed journal. The trial registration number is UMIN 000048966.
Does Farm wage Influence Gender gap in Household Welfare? A Microlevel Evidence from Nigeria
Authors: Daudu Abdulrazaq Kamal , Karakara Alhassan Abdul-Wakeel , Kareem Oyedola Waheed , Olatinwo Latifat Kehinde , Dolapo Tawakalitu Abiola , Egbewole Halimah Olayinka , Adefalu Lateef Lawal , Abdulwahab Sidiqat Adeyemi
Published: June 2024

Few studies have explored the gender wage gap among farmworkers, and there is a notable absence of research on how farm wages impact household welfare differently based on gender. This study aims to fill this gap by examining how farm wages affect gender disparities in household welfare among farmworkers in Nigeria. Using survey data from 720 farmworkers, we investigated gender-based differences in household welfare. Our approach combined parametric methods (Blinder-Oaxaca decomposition) to assess gender gaps in household welfare and non-parametric techniques (Propensity Score Matching, PSM) to estimate the effects of farm wages. The analysis reveals that female farmworkers experience significantly lower per capita food consumption expenditure (₦11,486.51 less) compared to their male counterparts. These disparities partly stem from differences in socioeconomic factors, job characteristics, and enterprise attributes between male and female farmworkers. The study underscores the role of farm wages in mitigating gender gaps in household welfare, demonstrating that farm wages have increased per capita food consumption expenditure for female farmworkers by ₦6,640.698. This finding highlights the potential of wage increases to enhance livelihoods, underscoring the importance of equitable wage policies in farming. Furthermore, our findings emphasize that the impact of farm wages on welfare varies with household characteristics, suggesting the need for targeted interventions tailored to enhance identified household attributes. Addressing these factors could effectively reduce gender inequalities in farm work and contribute to overall welfare improvements.
Changes in RANKL, OPG, and 25(OH)D Levels in Children with Leukemia from Diagnosis to Remission
Authors: Atilano-Miguel Salvador , Barbosa-Cortés Lourdes , Ortiz-Muñiz Rocío , Maldonado-Hernández Jorge , Martin-Trejo Jorge A , Rodríguez-Cruz Maricela , Balcázar-Hernández Lourdes , Solís-Labastida Karina A , Bautista-Martínez Benito A , Juárez-Moya Azalia , Hernández-Piñón Zayra , Galindo-Rodríguez Raeline A , Chávez Anaya Adriana , Valdez-Avilez Rosa E , Domínguez-Salgado Juan M , Villa-Morales Judith , Rodríguez-Palacios María E
Published: June 2024

Background: The receptor activator of the nuclear factor-kB (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) pathway is a determining pathway in the balance between bone formation and resorption; disruptions in this complex can affect bone metabolism. Methods: This study analyzed the changes in RANKL, OPG and 25(OH)D levels, the RANKL/OPG ratio, and other bone turnover markers (BTM) from diagnosis to complete remission in children with acute lym-phoblastic leukemia (ALL). This is a prospective observational cohort study. This study was carried out in the Instituto Mexicano del Seguro Social in Mexico City. Thirty-three patients (4-17 years) with newly diagnosed B-cell ALL were included. The patients were treated with the HP09 chemotherapy protocol. Children who had previously been treated with corticosteroids were excluded. A pe-ripheral blood sample at diagnosis and remission was collected to determine the 25(OH)D and BTM concentrations. Results: Increased RANKL (p=0.001) and osteocalcin (p
Culture of Bovine Aortic Endothelial Cells in Galactose Media Enhances Mitochondrial Plasticity and Changes Redox Sensing Altering Nrf2 and FOXO3 Levels
Authors: Galant Leticia Selinger , Doblado Laura , Radi Rafael , Bem Andreza Fabro de , Monsalve Maria
Published: June 2024

Understanding complex biological processes of cells in culture, particularly those related to metabolism, can be biased by culture conditions, since the choice of energy substrate impacts all main metabolic pathways. When glucose is replaced by galactose cells decrease their glycolytic flux, working as an in vitro model of limited nutrient availability. However, the effect of these changes on related physiological processes such as REDOX control is not well documented, particularly in endothelial cells where mitochondrial oxidation is considered to be low. We evaluated the differences in mitochondrial dynamics and function in endothelial cells exposed to galactose or glucose culture medium. We observed that cells maintained in galactose-containing medium show higher mitochondrial oxidative capacity, more fused mitochondrial network, and higher intercellular coupling. These factors are documented to impact the cellular response to oxidative stress. Therefore, we analyzed the levels of two main REDOX regulators and found that Bovine Aortic Endothelial Cells (BAEC) in galactose media had higher levels of FOXO3 and lower levels of Nrf2 than those with glucose in the media. Thus, culture of endothelial cells in galactose containing medium may provide a more suitable condition to study in vitro mitochondrial related processes than glucose media, and deeply influence REDOX signaling in these cells.
Development and Content Validation of a Clinical Screening Scale to Identify Attention Deficit Hyperactivity Disorder Considering the Gender Perspective: An eDelphi Study
Authors: Perez-Beltran Meritxell , Roldán-Merino Juan Francisco , Russi Maria Eugenia , Garau Maria , Colome Roura Roser , Sampaio Francisco , Dominguez Marta , Farrés Tarafa Mariona , Hurtado Barbara , Alda José Ángel
Published: June 2024

Background: Although many studies analyse gender differences in the clinical expression of Attention Deficit Hyperactivity Disorder (ADHD) and prevalence studies show that girls with ADHD are underdiagnosed, there are no instruments that are sensitive to the detection of girls with ADHD.
Objective: The objective of this study is to develop a self-report early detection instrument for ADHD for boys and girls aged 7 to 16, which includes the gender perspective and is sensitive to the detection of girls with ADHD.
Methods: The development of the scale and the items that comprise it were created from the thematic analysis of ADHD and its evaluation in children, based on the diagnostic criteria of the DSM-5-TR. A modified e-Delphi method involving a three-round web survey was used to reach a consensus on the content of the scale. Ten experts were recruited into a professional panel. The panel members were asked to assess the differential symptomatology of ADHD in boys and girls, the dimensions to be evaluated, and the importance of the scale items to evaluate the content.
Results: Consensus was reached regarding 13 total items distributed in three dimensions: Inattention, Hyperactivity/Impulsivity, and a third dimension, Internalisation, which includes symptoms most present in the expression of ADHD in girls.
Conclusions: To the best of our knowledge, the development of this scale using the Delphi method is the first specific scale for identifying ADHD that addresses the gender perspective and the differential symptomatology between boys and girls. Although we must proceed to the analysis of the psychometric properties, we can anticipate that this scale will provide relevant and reliable information for the identification of ADHD in both boys and girls.
Differences of Energy Balance and GHG Emissions in Legume Intercropped Maize Agroecosystem as a Result of Climate Change
Authors: Romaneckas Kęstutis , Švereikaitė Austėja , Kimbirauskienė Rasa , Sinkevičienė Aušra , Adamavičienė Aida , Jasinskas Algirdas
Published: June 2024

Multicropping can solve the energy and GHG balance problems, but the emergence, development and productivity of such mixed crops are at risk due to uneven distribution of precipitation. For this reason, investigations were performed at the Experimental Station of Vytautas Magnus University, Lithuania. Single maize crop was compared with Crimson/red clover, Persian clover, and alfalfa intercropped maize cultivations. Results showed that under arid conditions, the intercrops biomass was about 4 times less than under humid. Humid conditions were less suitable for maize and when growing with intercrops they produced about 3-5 t ha-1 less dried biomass, and when growing sole - about 6 t ha-1 less biomass than in arid conditions. Due to the higher yield of maize biomass in arid season, better energy indicators of the crop were obtained in arid conditions than in humid. The difference between net energy was about 122-123 MJ ha-1 in all treatments, except for the maize crop with intercropped alfalfa, where the difference was 62 MJ ha-1. All tested technologies were environmentally friendly, CO2 equivalent varied between treatments from 804 to 884 kg ha-1. Uneven distribution of precipitation during vegetative season makes us think about the improvement of intercropping technologies. Sowing intercrops at the same time with maize could improve its germination but increase the problem of weed spread.
Gambling and Gaming: A Comparative Study of Professional Footballers Versus the General Population
Authors: González-Moret Rafael , Almodóvar-Fernández Isabel , Gimeno María , Blanco Ana , Sánchez-Thevenet Paula , Usó Héctor , Haro Gonzalo , Real-Fernández Antonio
Published: June 2024

(1) Background: Elite sport can increase vulnerability to developing mental health pathologies. The purpose of the study is to determine the frequency at which these behavioural disorders appear in elite footballers and evaluate their relationship with other addictions. (2) Methods: A cross-sectional study was conducted between November 2020 and January 2022 on 306 participants. The variables of gambling and gaming were studied. The different groups were compared using Chi-squared tests. Probabilities exceeding 95% (p-values < 0.05) and residuals results greater than 2 or less than −2 were considered significant. (3) Results: There were significant differences between the two groups in terms of alcohol (p < 0.001), tobacco (p < 0.001), and cannabis (p < 0.018) consumption. We also found differences between those who had a history of a nervous disease (p < 0.015). 6.6% of the of football players had a probable diagnosis of gambling disorder compared to 1% in the general population (p < 0.007). Among the pathological and non-pathological cases of gambling in the football population, significant differences were found between those with a salary of €900–1,500/month (p < 0.033) or a history of a nervous pathology (p < 0.001). (4) Conclusions: This study showed that professional football players were vulnerable to mental health pathologies related to gaming.
Effect of Different Slow‐Release Urea on the Production Performance, Rumen Fermentation and Blood Parameter of Angus Heifer
Authors: Fan Caiyun , Li Hongguang , Li Shuaihong , Zhong Gang , Jia Wenbin , Zhuo Zhao , Xue Yanfeng , Koontz Anne F. , Cheng Jianbo
Published: June 2024

This study investigated the effect of replacing part of the dietary soybean meal with either polymer-coated urea or gelatinized starch urea on the performance, nutrient digestibility, blood biochemical indexes, ruminal fermentation and rumen microflora of Angus heifers. The urea release profiles of two kinds of slow-release urea were evaluated in vitro and indicated a superior nitrogen retention with the polymer-coated urea. 210 purebred Angus cattle (BW = 314.26 kg) were divided into three groups: no urea group (CON), polymer-coated urea group (PCU), gelatinized starch urea group (GSU), 20g/kg polymer-coated urea or 25g/kg gelatinized starch urea was used to replace part of soybean meal in the concentrate feed respectively according to the principle of iso-metabolic energy and iso-nitrogenous. During the whole experiment, the ADG of PCU group was higher than both the GSU group and the CON group. The feed-weight ratio of the PCU group was significantly lower than both the GSU group and the CON group during the 90-day feeding period. The ADF apparent digestibility in the PCU group was significantly higher than that of the CON group, and extremely significantly higher than that of the GSU group. On the 86th day, the ALB content in the PCU group was significantly higher than that in the CON group. The contents of acetic acid and butyric acid, total volatile fatty acid and propionic acid were significantly higher in the PCU group than in the CON group. Bacterial diversity analysis found that the abundance of Firmicutes was higher in the PCU group than that in the GSU group and the CON group at the phylum level, and inverse result was observed in Bacteroidetes. The abundance of Paraprevotella was higher in the PCU group, whereas higher abundance of Prevotella was found in the GSU group at the genus level. These results indicate that both polymer-coated urea and gelatinized starch urea can replace part of soybean meal in the diet, and the amount of substitution in this trial had no diverse effect on the performance of Angus heifers, and the effect and economic benefits brought by polymer-coated urea were better than gelatinized starch urea.
STAT4 Mediates IL-6 Trans-signaling Ventricular Arrhythmias in High Fat Diet Guinea Pig Heart
Authors: Corbin Andrea , Aromolaran Kelly Ann , Aromolaran Ademuyiwa S
Published: June 2024

Obesity is a major risk factor for the development of life-threatening malignant ventricular tachyarrhythmias (VT) and sudden cardiac death (SCD). Risks may be highest for patients with high levels of the proinflammatory cytokine interleukin (IL)-6. We used our guinea pig model of high-fat diet (HFD) induced Ventricular arrhythmias, that exhibit heightened proinflammatory-like pathology which is also observed in human obesity VT, as well as immunofluorescence, and confocal microscopy approaches to evaluate the pathological IL-6 trans-signaling function and explore the underlying mechanisms. Using blind-stick and electrocardiogram (ECG) techniques, we tested the hypothesis that heightened IL-6 trans-signaling exhibit enhanced Ventricular arrhythmias /SCD incidence and underlying arrhythmia substrates. Remarkably, compared to low-fat (LFD) diet fed controls, HFD promotes phosphorylation of the IL-6 signal transducer and activator of transcription 4 (STAT4) leading to its activation and enhanced nuclear translocation of pSTAT4/STAT4 more than LFD controls and pSTAT3/STAT3 nuclear expression. Overactivation of IL-6 trans-signaling in guinea pigs prolonged the QT interval that resulted in greater susceptibility to VT/SCD with isoproterenol challenge, as also observed with the downstream Janus kinase (JAK) 2 activator. These findings may have potentially profound implications for more effective VT therapy in the vulnerable obese patient population.
The Modified RoPE Score is Associated with Patent Foramen Ovale in Patients with Ischemic Stroke: A Nationwide US Analysis
Authors: Kwok Chun Shing , Alisiddiq Zaheer , Will Maximilian , Schwarz Konstantin , Khoo Chee , Large Adrian , Butler Robert , Lip Gregory YH , Qureshi Adnan I. , Borovac Josip Andelo
Published: June 2024

Background: The Risk of Paradoxical Embolism (RoPE) score was developed to identify stroke-related patent foramen ovale (PFO) in patients with cryptogenic stroke. Methods: We conducted a retrospective analysis of the 2016 to 2020 National Inpatient Sample to determine the performance of modified RoPE score for PFO presence in patients with acute ischemic stroke (AIS). Results: A total of 3,338,805 hospital admissions for AIS were analysed and 3.0% had PFO. Patients with PFO were younger compared to those without PFO (median 63 years vs. 71 years, p<0.001) and fewer were female (46.1% vs. 49.7%, p<0.001). The patients with PFO had greater mean modified RoPE score (4.0 vs. 3.3, p<0.001). The area under the curve for the RoPE score in predicting PFO was 0.625 (95%CI 0.620-0.629). The best diagnostic power of the RoPE score was achieved with a cut point of ≥4 where the sensitivity was 55% and the specificity was 64.2%. A cut point of ≥5 increased specificity (83.1%) at the expense of sensitivity (35.8%). The strongest predictor of PFO was deep vein thrombosis (OR 3.97, 95%CI 3.76-4.20). Conclusions: The modified RoPE score had modest predictive value in identifying patients with PFO among patients admitted with AIS.
The Impacts of Frozen Material-Other-Than-Grapes (MOG) on Aroma Compounds of Cabernet Franc and Cabernet Sauvignon
Authors: LAN YIBIN , Xu Xiaoyu , Wang Jiaming , Aubie Emily , Crombleholme Marnie , Reynolds Andrew
Published: June 2024

An undesirable sensory attribute (“floral taint”) has been recently detected in red wines in some winegrowing jurisdictions in North America (e.g. Ontario, British Columbia, Washington), caused by introduction of frost-killed leaves and petioles [materials-other-than-grapes (MOG)] during mechanical harvest and winemaking. It was hypothesized that terpenes, norisoprenoids, and higher alcohols would be the main responsible compounds. Objectives were to investigate causative volatile compounds for floral taint, and explore threshold concentrations for this problem. Commercial wines displaying varying intensities of floral taints were subjected to GC-MS and sensory analysis. Several odor-active compounds were higher in floral-tainted wines, including terpenes (geraniol, citronellol, cis- and trans-rose oxide), norisoprenoids (β-damascenone, β-ionone), five ethyl esters, and three alcohols. Thereafter, fermentations of Cabernet franc (CF) and Cabernet Sauvignon (CS) (2016, 2017) were conducted. MOG treatments were (wt/wt): 0, 0.5%, 1%, 2% and 5% petioles, and 0, 0.25%, 0.5%, 1%, and 2% leaf blades. Terpenes (linalool, geraniol, nerol, nerolidol, citronellol, citral, cis- and trans-rose oxides, eugenol, myrcene), norisoprenoids (α- and β-ionone), and others (e.g. hexanol, octanol, methyl and ethyl salicylate) increased linearly/quadratically with increasing MOG levels in both cultivars. Principal components analysis separated MOG treatments from the controls with 5% petioles and 2% leaves as extremes. Increasing MOG levels in CF wines increased floral aroma intensity, and were primarily associated with terpenes, higher alcohols, and salicylates. Increased leaf levels in CF were associated with higher vegetal and earthy attributes. Increased petioles in CS were not correlated with floral aromas, but increased leaves increased floral, vegetal and herbaceous attributes. Overall, petioles contributed more to floral taint than leaves through increased terpenes and salicylates (floral notes), while leaves contributed norisoprenoids and C6 alcohols (green notes).
Bubble dynamics in the Polyakov quark-meson model
Authors: Wang Junrong , Jin Jinshuang , Mao Hong
Published: June 2024

Based on the two-flavors Polyakov quark-meson model, we study the bubble dynamics of a first-order phase transition in chiral phase diagram at high density but low temperature in the framework of the homogeneous thermal nucleation theory. Under the mean-field approximation, after obtaining the effective potential with inclusion of the fermionic vacuum term, we build a geometric approach to probe two possibly existing minima, which can actually tunnel through a bounce interpolated between a metastable vacuum to an adjacent true vacuum. By separating our discussions into a weak first-order hadron quark phase transition and strong ones, the bubble profiles, the surface tension, the typical radius of the bounce and the saddle point action in the presence of a nucleation bubble as a function of temperature are numerically calculated in detail as the chemical potentials are fixed at $\mu=306 \mathrm{MeV}$ and $\mu=310 \mathrm{MeV}$. our results show that the surface tension remains a small value even when the density is very high and phase boundaries for hadron and quark matter should be resized properly according to the saddle point action evaluated on the bounce. It is also noticed that the inclusion of the deconfinement phase transition in term of the Polyakov loop does not change chiral phase transition dramatically for light quarks.
Examining How Diet and Lifestyle Influence Skin Appearance Through a Common Risk Factor: Excess Iron—A Comprehensive Review
Authors: Yang Tianshu , Chen Lung-Chi , Huang Xi
Published: June 2024

In the contemporary era, youthful and healthy skin is a pivotal determinant of beauty. Choices pertaining to one's dietary and lifestyle practices wield substantial influence over skin health. Currently, the focal point of attention lies in strategies to delay skin aging and maintain skin quality. Remarkably, the skin, the body's largest organ, serves as the primary defense barrier against external elements. Skin aging encompasses intrinsic and extrinsic categories, both susceptible to genetic, lifestyle, and environmental factors. Given the strides in science and technology, the pursuit of effective and safe interventions for skin aging assumes paramount importance. Thus, this review delves into the intricate relationship between diet, lifestyle, and skin aging, culminating in an exploration of the crucial role played by excess iron in this intricate nexus. Understanding these dynamics holds promise for advancing our knowledge of skincare and the quest for timeless vitality.
Phage Therapy: An Alternative Approach to Combat Multi-drug Resistant Infections in Cystic Fibrosis
Authors: Cocorullo Mario , Stelitano Giovanni , Chiarelli Laurent Robert
Published: June 2024

In this review we aim to address the current status of phage therapy in the management of multidrug-resistant infections, from compassionate use cases to ongoing clinical trials, as well as the challenges this approach presents in the particular context of CF patients.
Controlled Hypoxia Acutely Prevents Physical Inactivity-Induced Peripheral BDNF Decline
Authors: Duderstadt Yves , Schreiber Stefanie , Burtscher Johannes , Schega Lutz , Müller Notger G. , Brigadski Tanja , Braun-Dullaeus Rüdiger , Leßmann Volkmar , Müller Patrick
Published: June 2024

: Background: Brain-derived neurotrophic factor (BDNF) is a crucial mediator of neuronal plasticity. Here, we investigated the effects of controlled normobaric hypoxia (NH) combined with physical inactivity on BDNF blood levels and executive functions. Methods: Twenty-five healthy adults (25.8±3.3 years, 15 female) were analyzed in a randomized, controlled cross-over study. Each intervention began with a 30 min resting phase under normoxia (NOR), followed by a 90 min continuation of NOR or NH (peripheral oxygen saturation [SpO2] 85-80%). Serum and plasma samples were collected every 15 min. Heart rate and SpO2 were continuously measured. Before and after each exposure, cognitive tests were performed and after 24 h another follow-up blood sample was taken. Results: NH decreased SpO2 (p<.001, ηp²=.747) and increased heart rate (p=.006, ηp²=.116) significantly. The 30-min resting phase under NOR led to a significant BDNF reduction in serum (p <.001, ηp²=.581) and plasma (p <.001, ηp²=.362). Continuation of NOR further significantly reduced BDNF after another 45 min (p=.018) in serum and after 30 min (p=.040) and 90 min (p=.005) in plasma. There was no significant BDNF decline under NH. 24 h Follow-up examination showed a significant decline of serum BDNF, both after NH and NOR. Conclusion: Our results show that NH has the potential to counteract physical inactivity-induced BDNF decline. Therefore, our study emphasizes the need for a physically active lifestyle and its positive effects on BDNF. This study also demonstrates the need for a standardized protocol for future studies to determine BDNF in serum and plasma.