bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Review of the Legal Considerations Surrounding Medicines Management
Authors: Vaismoradi Mojtaba , Jordan Sue , Logan Patricia A. , Amaniyan Sara , Glarcher Manela
Published: January 2021

Background and Objectives: There is a paucity of integrated knowledge regarding legal considerations required to ensure patient safety through safe medicines management. This study explores the legal considerations surrounding medicines management, providing a synthesis of existing knowledge. An integrative systematic review of the current international knowledge was performed. Materials and Methods: The search encompassed the online databases of PubMed (including Medline), Scopus, CINAHL, and Web of Science using MeSH terms and relevant keywords relating to the legal considerations of medicines management in healthcare settings. Results: The search process led to the identification of 6051 studies published between 2010 and 2020, of which six articles were found to be appropriate for data analysis and synthesis based on inclusion criteria. Research methods were varied and included qualitative interviews, mixed-methods designs, retrospective case reports and cross-sectional interrupted time-series analysis. Their foci were on the delegation of medicines management, pharmacovigilance and reporting of adverse drug reactions (ADRs) before and after legislation by nurses, physicians and pharmacists, medico-legal litigation, use of forced medication and the prescription monitoring program. Given the heterogenicity of the studies in terms of aims and research methods, a meta-analysis could not be performed and, therefore, our review findings are presented narratively under the categories of ‘healthcare providers’ education and monitoring tasks’, ‘individual and shared responsibility’, and ‘patients’ rights’. Conclusion: This review identifies legal aspects surrounding medicines management, including supervision and monitoring of the effects of medicines; healthcare providers’ knowledge and attitudes; support and standardised tools for monitoring and reporting medicines’ adverse side effects/ADRs; electronic health record systems; individual and shared perceptions of responsibility; recognition of nurses’ roles; detection of sentinel medication errors; covert or non-voluntary administration of medication, and patient participation.
Effects of Hexane on Protein Profile, Solubility and Foaming Properties of Defatted Proteins Extracted from Tenebrio molitor Larvae
Authors: Gravel Alexia , Marciniak Alice , Couture Manon , Doyen Alain
Published: January 2021

Inclusion of edible insects in human diets is increasingly promoted as a sustainable source of proteins with high nutritional value. While consumer acceptability remains the main challenge to their integration into Western food culture, the use of edible insects as meal and protein concentrate could decrease neophobia. The defatting of edible insects, mostly done with hexane, is the first step in producing protein ingredients. However, its impact on protein profiles and techno-functionality is still unclear. Consequently, this study compares the protein profiles of hexane-defatted and non-hexane-defatted yellow mealworm (Tenebrio molitor) meals and protein extracts, and evaluates the impact of hexane on protein solubility and foaming properties. Results showed that profiles for major proteins were similar between hexane-defatted and non-defatted samples, however some specific content differences (e.g., hexamerin 2) were observed and characterized using proteomic tools. Protein solubility was markedly lower for T. molitor meals compared to protein extracts. A large increase in the foaming capacity was observed for defatted fractions, whereas foam stability decreased similarly in all fractions. Consequently, although the hexane-defatting step was largely studied to produce edible insect protein ingredients, it is necessary to precisely understand its impact on their techno-functional properties for the development of food formulations.
Catalysis by Hybrid Nanomaterials
Authors: Kustov Leonid M.
Published: January 2021

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Room Temperature Ionic Liquids in Asymmetric Hetero-Ene Type Reactions: Improving Organocatalyst Performance at Lower Temperatures
Authors: Bisogno Fabricio R. , Fernández Rosario , Lassaletta Jose María , de Gonzalo Gonzalo de
Published: January 2021

Room temperature ionic liquids (RTILs) have been widely used as (co)solvents in several catalytic processes modifying, in most of the cases, the catalyst activity and/or the selectivity for the studied reactions. However, there are just a few examples of their use in hydrogen bonding organocatalysis. In this paper, we show the positive effect of a set of imidazole-based ionic liquids ([bmim]BF4 and [hmim]PF6) in the enantioselective addition of formaldehyde tert-butylhydrazone to prochiral α-keto esters catalyzed by a sugar-based chiral thiourea. Reactions performed in the presence of low percentages of RTILs led to an increase of the catalyst activity, thereby making possible to work at lower temperatures. Thus, the chiral tert-butyl azomethyl tertiary alcohols could be obtained with moderate to good conversions and higher enantioselectivities for most of the studied substrates when using up to 30 vol% of [hmim]PF6 as a cosolvent in processes performed in toluene.
Cytokinin Plant Hormones Have Neuroprotective Activity in In Vitro Models of Parkinson’s Disease
Authors: Gonzalez Gabriel , Grúz Jiří , D’Acunto Cosimo Walter , Kaňovský Petr , Strnad Miroslav
Published: January 2021

Cytokinins are adenine-based phytohormones that regulate key processes in plants, such as cell division and differentiation, root and shoot growth, apical dominance, branching, and seed germination. In preliminary studies, they have also shown protective activities against human neurodegenerative diseases. To extend knowledge of the protection (protective activity) they offer, we investigated activities of natural cytokinins against salsolinol (SAL)-induced toxicity (a Parkinson’s disease model) and glutamate (Glu)-induced death of neuron-like dopaminergic SH-SY5Y cells. We found that kinetin-3-glucoside, cis-zeatin riboside, and N6-isopentenyladenosine were active in the SAL-induced PD model. In addition, trans-, cis-zeatin, and kinetin along with the iron chelator deferoxamine (DFO) and the necroptosis inhibitor necrostatin 1 (NEC-1) significantly reduced cell death rates in the Glu-induced model. Lactate dehydrogenase assays revealed that the cytokinins provided lower neuroprotective activity than DFO and NEC-1. Moreover, they reduced apoptotic caspase-3/7 activities less strongly than DFO. However, the cytokinins had very similar effects to DFO and NEC-1 on superoxide radical production. Overall, they showed protective activity in the SAL-induced model of parkinsonian neuronal cell death and Glu-induced model of oxidative damage mainly by reduction of oxidative stress.
Effects of Total Thermal Balance on the Thermal Energy Absorbed or Released by a High-Temperature Phase Change Material
Authors: Rodríguez-Alemán Suset , Hernández-Cooper Ernesto M. , Pérez-Álvarez Rolando , Otero José A.
Published: January 2021

Front tracking and enthalpy methods used to study phase change processes are based on a local thermal energy balance at the liquid–solid interface where mass accommodation methods are also used to account for the density change during the phase transition. Recently, it has been shown that a local thermal balance at the interface does not reproduce the thermodynamic equilibrium in adiabatic systems. Total thermal balance through the entire liquid–solid system can predict the correct thermodynamic equilibrium values of melted (solidified) mass, system size, and interface position. In this work, total thermal balance is applied to systems with isothermal–adiabatic boundary conditions to estimate the sensible and latent heat stored (released) by KNO3 and KNO3/NaNO3 salts which are used as high-temperature phase change materials. Relative percent differences between the solutions obtained with a local thermal balance at the interface and a total thermal balance for the thermal energy absorbed or released by high-temperature phase change materials are obtained. According to the total thermal balance proposed, a correction to the liquid–solid interface dynamics is introduced, which accounts for an extra amount of energy absorbed or released during the phase transition. It is shown that melting or solidification rates are modified by using a total thermal balance through the entire system. Finally, the numerical and semi-analytical methods illustrate that volume changes and the fraction of melted (solidified) solid (liquid) estimated through a local thermal balance at the interface are not invariant in adiabatic systems. The invariance of numerical and semi-analytical solutions in adiabatic systems is significantly improved through the proposed model.
An Efficient Synthesis of Optically Active [4-13C] Labelled Quorum Sensing Signal Autoinducer-2
Authors: Ascenso Osvaldo S. , Carrau Gonzalo , Xavier Karina B. , Ventura M. Rita , Maycock Christopher D.
Published: January 2021

A new synthetic route for the quorum sensing signal Autoinducer-2 (AI-2) is described and used for the preparation of [4-13C]-AI-2 starting from [1-13C]-bromoacetic acid. The key step in this process was the enantioselective reduction of an intermediate ketone. This synthesis provides, selectively, both enantiomers of the labelled or unlabelled parent compound, (R) or (S)-4,5-dihydroxypentane-2,3-dione (DPD) and was used for an improved synthesis of [1-13C]-AI-2.
Novel Fluorinated 7-Hydroxycoumarin Derivatives Containing an Oxime Ether Moiety: Design, Synthesis, Crystal Structure and Biological Evaluation
Authors: Wang Qing-Qing , Zhang Shu-Guang , Jiao Jian , Dai Peng , Zhang Wei-Hua
Published: January 2021

A series of fluorinated 7-hydroxycoumarin derivatives containing an oxime ether moiety have been designed, synthesized and evaluated for their antifungal activity. All the target compounds were determined by 1H-NMR, 13C-NMR, FTIR and HR-MS spectra. The single-crystal structures of compounds 4e, 4h, 5h and 6c were further confirmed using X-ray diffraction. The antifungal activities against Botrytis cinerea (B. cinerea), Alternariasolani (A. solani), Gibberella zeae (G. zeae), Rhizoctorzia solani (R. solani), Colletotrichum orbiculare (C. orbiculare) and Alternaria alternata (A. alternata) were evaluated in vitro. The preliminary bioassays showed that some of the designed compounds displayed the promising antifungal activities against the above tested fungi. Strikingly, the target compounds 5f and 6h exhibited outstanding antifungal activity against B. cinerea at 100 μg/mL, with the corresponding inhibition rates reached 90.1 and 85.0%, which were better than the positive control Osthole (83.6%) and Azoxystrobin (46.5%). The compound 5f was identified as the promising fungicide candidate against B. cinerea with the EC50 values of 5.75 μg/mL, which was obviously better than Osthole (33.20 μg/mL) and Azoxystrobin (64.95 μg/mL). Meanwhile, the compound 5f showed remarkable antifungal activities against R. solani with the EC50 values of 28.96 μg/mL, which was better than Osthole (67.18 μg/mL) and equivalent to Azoxystrobin (21.34 μg/mL). The results provide a significant foundation for the search of novel fluorinated 7-hydroxycoumarin derivatives with good antifungal activity.
Ellagitannin–Lipid Interaction by HR-MAS NMR Spectroscopy
Authors: Virtanen Valtteri , Räikkönen Susanna , Puljula Elina , Karonen Maarit
Published: January 2021

Ellagitannins have antimicrobial activity, which might be related to their interactions with membrane lipids. We studied the interactions of 12 different ellagitannins and pentagalloylglucose with a lipid extract of Escherichia coli by high-resolution magic angle spinning NMR spectroscopy. The nuclear Overhauser effect was utilized to measure the cross relaxation rates between ellagitannin and lipid protons. The shifting of lipid signals in 1H NMR spectra of ellagitannin–lipid mixture due to ring current effect was also observed. The ellagitannins that showed interaction with lipids had clear structural similarities. All ellagitannins that had interactions with lipids had glucopyranose cores. In addition to the central polyol, the most important structural feature affecting the interaction seemed to be the structural flexibility of the ellagitannin. Even dimeric and trimeric ellagitannins could penetrate to the lipid bilayers if their structures were flexible with free galloyl and hexahydroxydiphenoyl groups.
Improving the Cellular Selectivity of a Membrane-Disrupting Antimicrobial Agent by Monomer Control and by Taming
Authors: Regen Steven L.
Published: January 2021

Antimicrobial resistance represents a significant world-wide health threat that is looming. To meet this challenge, new classes of antimicrobial agents and the redesign of existing ones will be required. This review summarizes some of the studies that have been carried out in my own laboratories involving membrane-disrupting agents. A major discovery that we made, using a Triton X-100 as a prototypical membrane-disrupting molecule and cholesterol-rich liposomes as model systems, was that membrane disruption can occur by two distinct processes, depending on the state of aggregation of the attacking agent. Specifically, we found that monomers induced leakage, while attack by aggregates resulted in a catastrophic rupture of the membrane. This discovery led us to design of a series of derivatives of the clinically important antifungal agent, Amphotericin B, where we demonstrated the feasibility of separating antifungal from hemolytic activity by decreasing the molecule’s tendency to aggregate, i.e., by controlling its monomer concentration. Using an entirely different approach (i.e., a “taming” strategy), we found that by covalently attaching one or more facial amphiphiles (“floats”) to Amphotericin B, its aggregate forms were much less active in lysing red blood cells while maintaining high antifungal activity. The possibility of applying such “monomer control” and “taming” strategies to other membrane-disrupting antimicrobial agents is briefly discussed.
Berberine Inhibits Telomerase Activity and Induces Cell Cycle Arrest and Telomere Erosion in Colorectal Cancer Cell Line, HCT 116
Authors: Samad Muhammad Azizan , Saiman Mohd Zuwairi , Abdul Majid Nazia , Karsani Saiful Anuar , Yaacob Jamilah Syafawati
Published: January 2021

Colorectal cancer (CRC) is the most common cancer among males and females, which is associated with the increment of telomerase level and activity. Some plant-derived compounds are telomerase inhibitors that have the potential to decrease telomerase activity and/or level in various cancer cell lines. Unfortunately, a deeper understanding of the effects of telomerase inhibitor compound(s) on CRC cells is still lacking. Therefore, in this study, the aspects of telomerase inhibitors on a CRC cell line (HCT 116) were investigated. Screening on HCT 116 at 48 h showed that berberine (10.30 ± 0.89 µg/mL) is the most effective (lowest IC50 value) telomerase inhibitor compared to boldine (37.87 ± 3.12 µg/mL) and silymarin (>200 µg/mL). Further analyses exhibited that berberine treatment caused G0/G1 phase arrest at 48 h due to high cyclin D1 (CCND1) and low cyclin-dependent kinase 4 (CDK4) protein and mRNA levels, simultaneous downregulation of human telomerase reverse transcriptase (TERT) mRNA and human telomerase RNA component (TERC) levels, as well as a decrease in the TERT protein level and telomerase activity. The effect of berberine treatment on the cell cycle was time dependent as it resulted in a delayed cell cycle and doubling time by 2.18-fold. Telomerase activity and level was significantly decreased, and telomere erosion followed suit. In summary, our findings suggested that berberine could decrease telomerase activity and level of HCT 116, which in turn inhibits the proliferative ability of the cells.
The Application of NMR Spectroscopy and Chemometrics in Authentication of Spices
Authors: Pacholczyk-Sienicka Barbara , Ciepielowski Grzegorz , Albrecht Łukasz
Published: January 2021

Spices and herbs are among the most commonly adulterated food types. This is because spices are widely used to process food. Spices not only enhance the flavor and taste of food, but they are also sources of numerous bioactive compounds that are significantly beneficial for health. The healing effects of spices are connected with their antimicrobial, anti-inflammatory and carminative properties. However, regular consumption of adulterated spices may cause fatal damage to our system because adulterants in most cases are unhealthy. For that reason, the appropriate analytical methods are necessary for quality assurance and to ensure the authenticity of spices. Spectroscopic methods are gaining interest as they are fast, require little or no sample preparation, and provide rich structural information. This review provides an overview of the application of NMR spectroscopy combined with chemometric analysis to determine the quality and adulteration of spices.
Recent Advances in Nanomedicine for the Diagnosis and Treatment of Prostate Cancer Bone Metastasis
Authors: Hagaman Daniel E. , Damasco Jossana A. , Perez Joy Vanessa D. , Rojo Raniv D. , Melancon Marites P.
Published: January 2021

Patients with advanced prostate cancer can develop painful and debilitating bone metastases. Currently available interventions for prostate cancer bone metastases, including chemotherapy, bisphosphonates, and radiopharmaceuticals, are only palliative. They can relieve pain, reduce complications (e.g., bone fractures), and improve quality of life, but they do not significantly improve survival times. Therefore, additional strategies to enhance the diagnosis and treatment of prostate cancer bone metastases are needed. Nanotechnology is a versatile platform that has been used to increase the specificity and therapeutic efficacy of various treatments for prostate cancer bone metastases. In this review, we summarize preclinical research that utilizes nanotechnology to develop novel diagnostic imaging tools, translational models, and therapies to combat prostate cancer bone metastases.
Visible-Light-Curable Solvent-Free Acrylic Pressure-Sensitive Adhesives via Photoredox-Mediated Radical Polymerization
Authors: Back Jong-Ho , Kwon Yonghwan , Kim Hyun-Joong , Yu Youngchang , Lee Wonjoo , Kwon Min Sang
Published: January 2021

Owing to their excellent properties, such as transparency, resistance to oxidation, and high adhesivity, acrylic pressure-sensitive adhesives (PSAs) are widely used. Recently, solvent-free acrylic PSAs, which are typically prepared via photopolymerization, have attracted increasing attention because of the current strict environmental regulations. UV light is commonly used as an excitation source for photopolymerization, whereas visible light, which is safer for humans, is rarely utilized. In this study, we prepared solvent-free acrylic PSAs via visible light-driven photoredox-mediated radical polymerization. Three α-haloesters were used as additives to overcome critical shortcomings, such as the previously reported low film curing rate and poor transparency observed during additive-free photocatalytic polymerization. The film curing rate was greatly increased in the presence of α-haloesters, which lowered the photocatalyst loadings and, hence, improved the film transparency. These results confirmed that our method could be widely used to prepare general-purpose solvent-free PSAs—in particular, optically clear adhesives for electronics.
Controversy of Peptide Cyclization from Tripeptide
Authors: Lin Chung-Yin , Chakraborty Subrata , Wong Chia-Wei , Tai Dar-Fu
Published: January 2021

The present investigation reports an attempt to synthesize naturally occurring α-cyclic tripeptide cyclo(Gly-l-Pro-l-Glu) 1, [cyclo(GPE)], previously isolated from the Ruegeria strain of bacteria with marine sponge Suberites domuncula. Three linear precursors, Boc-GPE(OBn)2, Boc-PE(OBn)G and Boc-E(OBn)GP, were synthesized using a solution phase peptide coupling protocol. Although cyclo(GPE) 1 was our original target, all precursors were dimerized and cyclized at 0 °C with high dilution to form corresponding α-cyclic hexapeptide, cyclo(GPE(OBn))27, which was then converted to cyclic hexapeptide cyclo(GPE)22. Cyclization at higher temperature induced racemization and gave cyclic tripeptide cyclo(GPDE(OBn)) 9. Structure characteristics of the newly synthesized cyclopeptides were determined using 1H-NMR, 13C-NMR and high-resolution mass spectrometry. The chemical shift values of carbonyls of 2 and 7 are larger than 170 ppm, indicating the formation of a cyclic hexapeptide.
Design, Synthesis and Biological Evaluation of Arylpyridin-2-yl Guanidine Derivatives and Cyclic Mimetics as Novel MSK1 Inhibitors. An Application in an Asthma Model
Authors: Bollenbach Maud , Nemska Simona , Wagner Patrick , Camelin Guillaume , Daubeuf François , Obrecht Adeline , Villa Pascal , Rognan Didier , Bihel Frédéric , Bourguignon Jean-Jacques , Schmitt Martine , Frossard Nelly
Published: January 2021

Mitogen- and Stress-Activated Kinase 1 (MSK1) is a nuclear kinase, taking part in the activation pathway of the pro-inflammatory transcription factor NF-kB and is demonstrating a therapeutic target potential in inflammatory diseases such as asthma, psoriasis and atherosclerosis. To date, few MSK1 inhibitors were reported. In order to identify new MSK1 inhibitors, a screening of a library of low molecular weight compounds was performed, and the results highlighted the 6-phenylpyridin-2-yl guanidine (compound 1a, IC50~18 µM) as a starting hit for structure-activity relationship study. Derivatives, homologues and rigid mimetics of 1a were designed, and all synthesized compounds were evaluated for their inhibitory activity towards MSK1. Among them, the non-cytotoxic 2-aminobenzimidazole 49d was the most potent at inhibiting significantly: (i) MSK1 activity, (ii) the release of IL-6 in inflammatory conditions in vitro (IC50~2 µM) and (iii) the inflammatory cell recruitment to the airways in a mouse model of asthma.
Evaluation of the Interplay between the ADAR Editome and Immunotherapy in Melanoma
Authors: Tusup Marina , Cheng Phil , Picardi Ernesto , Raziunaite Austeja , Dummer Reinhard , Levesque Mitchell , French Lars , Guenova Emmanuella , Kundig Thomas , Pascolo Steve
Published: January 2021

Background: RNA editing is a highly conserved posttranscriptional mechanism that contributes to transcriptome diversity. In mammals, it includes nucleobase deaminations that convert cytidine (C) into uridine (U) and adenosine (A) into inosine (I). Evidence from cancer studies indicates that RNA-editing enzymes promote certain mechanisms of tumorigenesis. On the other hand, recoding editing in mRNA can generate mutations in proteins that can participate in the Major Histocompatibility Complex (MHC) ligandome and can therefore be recognized by the adaptive immune system. Anti-cancer treatment based on the administration of immune checkpoint inhibitors enhance these natural anti-cancer immune responses. Results: Based on RNA-Seq datasets, we evaluated the editome of melanoma cell lines generated from patients pre- and post-immunotherapy with immune checkpoint inhibitors. Our results reveal a differential editing in Arthrobacter luteus (Alu) sequences between samples pre-therapy and relapses during therapy with immune checkpoint inhibitors. Conclusion: These data pave the way towards the development of new diagnostics and therapies targeted to editing that could help in preventing relapses during immunotherapies.
Drought Effects on Nitrogen Provisioning in Different Agricultural Systems: Insights Gained and Lessons Learned from a Field Experiment
Authors: Kundel Dominika , Lori Martina , Fliessbach Andreas , van Kleunen Mark , Meyer Svenja , Mäder Paul
Published: January 2021

Most nitrogen (N) in organic fertilizers must be mineralized to become available to plants, a process in which microorganisms play crucial roles. Droughts may impact microorganisms associated with the N cycle, negatively affecting N mineralization and plant N supply. The effects of drought on N-related processes may further be shaped by the farming system. We buried 15N-enriched plant material and reduced precipitation in conventionally and organically (biodynamically) managed wheat fields. On two sampling dates, we evaluated the soil water content, plant parameters and the plants’ 15N isotope signature. We intended to study the microbial communities associated with the N cycle to link potential treatment effects on plant N provisioning with characteristics of the underlying microbial community. However, floods impaired the experiment after the first sampling date, and the molecular work on the microbial communities was not performed. Focusing on the pre-flooding sampling date, our data suggested that processes associated with N transformation are sensitive to drought, but the role of the farming system needs further investigation. Since the underlying research question, the set-up and the lessons learned from this study may guide future experiments, we presented improvements to the set-up and provided ideas for additional analyses, hoping to promote research on this topic.
Lower Levels of Transaminases but Higher Levels of Serum Creatinine in Patients with Acute Hepatitis E in Comparison to Patients with Hepatitis A
Authors: Brehm Thomas Theo , Mazaheri Omid , Horvatits Thomas , Lütgehetmann Marc , Schulze zur Wiesch Julian , Lohse Ansgar W. , Polywka Susanne , Pischke Sven
Published: January 2021

In patients with hepatitis E virus (HEV) infections, extrahepatic, particularly renal and hematological manifestations, are increasingly reported in the medical literature but have never been studied compared to a control cohort. We retrospectively analyzed medical records of consecutive patients that were diagnosed with acute hepatitis E (AHE) (n = 69) or acute hepatitis A (AHA) (n = 46) at the University Medical Center Hamburg Eppendorf from January 2009 to August 2019 for demographical, clinical, and laboratory information. Patients with AHE had significantly lower median levels of ALAT (798 U/L) and total bilirubin (1.8 mg/dL) compared to patients with AHA (2326 U/L; p < 0.001 and 5.2 mg/dL; p < 0.001), suggesting a generally less severe hepatitis. In contrast, patients with AHE had significantly higher median serum creatinine levels (0.9 mg/dL vs. 0.8 mg/dL; p = 0.002) and lower median estimated glomerular filtration rate (eGFR) (91 mL/min/1.73 m2 vs. 109 mL/min/1.73 m2; p < 0.001) than patients with AHA. Leucocyte, neutrophil and lymphocyte count, hemoglobin, platelets, red cell distribution width (RDW), neutrophil to lymphocyte ratio (NLR), and RDW to lymphocyte ratio (RLR) did not differ between patients with AHE and those with AHA. Our observations indicate that renal but not hematological interference presents an underrecognized extrahepatic feature of AHE, while inflammation of the liver seems to be more severe in AHA.
Assessment of the Current Status of Potyviruses in Watermelon and Pumpkin Crops in Spain: Epidemiological Impact of Cultivated Plants and Mixed Infections
Authors: De Moya-Ruiz Celia , Rabadán Pilar , Juárez Miguel , Gómez Pedro
Published: January 2021

Viral infections on cucurbit plants cause substantial quality and yield losses on their crops. The diseased plants can often be infected by multiple viruses, and their epidemiology may depend, in addition to the agro-ecological management practices, on the combination of these viral infections. Watermelon mosaic virus (WMV) is one of the most prevalent viruses in cucurbit crops, and Moroccan watermelon mosaic virus (MWMV) emerged as a related species that threatens these crops. The occurrence of WMV and MWMV was monitored in a total of 196 apical-leaf samples of watermelon and pumpkin plants that displayed mosaic symptoms. The samples were collected from 49 fields in three major cucurbit-producing areas in Spain (Castilla La-Mancha, Alicante, and Murcia) for three consecutive (2018–2020) seasons. A molecular hybridization dot-blot method revealed that WMV was mainly (53%) found in both cultivated plants, with an unadvertised occurrence of MWMV. To determine the extent of cultivated plant species and mixed infections on viral dynamics, two infectious cDNA clones were constructed from a WMV isolate (MeWM7), and an MWMV isolate (ZuM10). Based on the full-length genomes, both isolates were grouped phylogenetically with the Emergent and European clades, respectively. Five-cucurbit plant species were infected steadily with either WMV or MWMV cDNA clones, showing variations on symptom expressions. Furthermore, the viral load varied depending on the plant species and infection type. In single infections, the WMV isolate showed a higher viral load than the MWMV isolate in melon and pumpkin, and MWMV only showed higher viral load than the WMV isolate in zucchini plants. However, in mixed infections, the viral load of the WMV isolate was greater than MWMV isolate in melon, watermelon and zucchini, whereas MWMV isolate was markedly reduced in zucchini. These results suggest that the impaired distribution of MWMV in cucurbit crops may be due to the cultivated plant species, in addition to the high prevalence of WMV.
Investigation of Coalescence-Induced Droplet Jumping on Mixed-Wettability Superhydrophobic Surfaces
Authors: Liao Ming-Jun , Duan Li-Qiang
Published: January 2021

Coalescence-induced droplet jumping has received more attention recently, because of its potential applications in condensation heat transfer enhancement, anti-icing and self-cleaning, etc. In this paper, the molecular dynamics simulation method is applied to study the coalescence-induced jumping of two nanodroplets with equal size on the surfaces of periodic strip-like wettability patterns. The results show that the strip width, contact angle and relative position of the center of two droplets are all related to the jumping velocity, and the jumping velocity on the mixed-wettability superhydrophobic surfaces can exceed the one on the perfect surface with a 180° contact angle on appropriately designed surfaces. Moreover, the larger both the strip width and the difference of wettability are, the higher the jumping velocity is, and when the width of the hydrophilic strip is fixed, the jumping velocity becomes larger with the increase of the width of the hydrophobic strip, which is contrary to the trend of fixing the width of the hydrophobic strip and altering the other strip width.
The Assessment of Hydrologic- and Flood-Induced Land Deformation in Data-Sparse Regions Using GRACE/GRACE-FO Data Assimilation
Authors: Tangdamrongsub Natthachet , Šprlák Michal
Published: January 2021

The vertical motion of the Earth’s surface is dominated by the hydrologic cycle on a seasonal scale. Accurate land deformation measurements can provide constructive insight into the regional geophysical process. Although the Global Positioning System (GPS) delivers relatively accurate measurements, GPS networks are not uniformly distributed across the globe, posing a challenge to obtaining accurate deformation information in data-sparse regions, e.g., Central South-East Asia (CSEA). Model simulations and gravity data (from the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GRACE-FO)) have been successfully used to improve the spatial coverage. While combining model estimates and GRACE/GRACE-FO data via the GRACE/GRACE-FO data assimilation (DA) framework can potentially improve the accuracy and resolution of deformation estimates, the approach has rarely been considered or investigated thus far. This study assesses the performance of vertical displacement estimates from GRACE/GRACE-FO, the PCRaster Global Water Balance (PCR-GLOBWB) hydrology model, and the GRACE/GRACE-FO DA approach (assimilating GRACE/GRACE-FO into PCR-GLOBWB) in CSEA, where measurements from six GPS sites are available for validation. The results show that GRACE/GRACE-FO, PCR-GLOBWB, and GRACE/GRACE-FO DA accurately capture regional-scale hydrologic- and flood-induced vertical displacements, with the correlation value and RMS reduction relative to GPS measurements up to 0.89 and 53%, respectively. The analyses also confirm the GRACE/GRACE-FO DA’s effectiveness in providing vertical displacement estimates consistent with GRACE/GRACE-FO data while maintaining high-spatial details of the PCR-GLOBWB model, highlighting the benefits of GRACE/GRACE-FO DA in data-sparse regions.
Application of Ecosystem Service Bundles and Tour Experience in Land Use Management: A Case Study of Xiaohuangshan Mountain (China)
Authors: Zhao Qiqi , Chen Yanming , Cuan Yuda , Zhang Han , Li Wei , Wan Sida , Li Manchun
Published: January 2021

With the deterioration of human-terrestrial relations and the intensification of global warming, development in all countries is facing difficulties. Whether in highly urbanized countries or in rapidly urbanizing developing countries such as China, the research on ecosystem services (ES) and land use management has attracted increasing attention. The general management of land use unilaterally pursues economic benefits and neglects ecological benefits, which aggravates the disparity between ecological development and the economic benefits of land resources. How to strike up a balance between ecologic protection and economic development remains a difficult problem during urbanization. It may be a better choice to formulate regional development strategies by combining natural conditions with humanistic and social tendencies. Identifying regional cultural ecosystem services (CES) and other important ES while performing zoning planning for regional land use can be a viable approach in land use management. Here, our study quantitatively evaluates the tourism experience of Xiaohuangshan Mountain (XHSM) and various ES, including recreation, biodiversity, history, aesthetics, soil conservation, surface water regulation, and soil nutrition. All ES were classified into four bundles for XHSM. Different ES bundles generated are suitable for different land use management methods and development forms according to their outstanding ES. The results show that quantifying and mapping regional ES bundles can provide the necessary information to support a win-win solution and provide decision support for land and spatial planning in areas with different social and ecological characteristics.
Design and Analysis of Super Wideband Antenna for Microwave Applications
Authors: Balani Warsha , Sarvagya Mrinal , Samasgikar Ajit , Ali Tanweer , Kumar Pradeep
Published: January 2021

In this article, a compact concentric structured monopole patch antenna for super wideband (SWB) application is proposed and investigated. The essential characteristics of the designed antenna are: (i) to attain super-wide bandwidth characteristics, the proposed antenna is emerged from a traditional circular monopole antenna and has obtained an impedance bandwidth of 38.9:1 (ii) another important characteristic of the presented antenna is its larger bandwidth dimension ratio (BDR) value of 6596 that is accomplished by augmenting the electrical length of the patch. The electrical dimension of the proposed antenna is 0.18λ×0.16λ (λ corresponds to the lower end operating frequency). The designed antenna achieves a frequency range from 1.22 to 47.5 GHz with a fractional bandwidth of 190% and exhibiting S11 < −10 dB in simulation. For validating the simulated outcomes, the antenna model is fabricated and measured. Good conformity is established between measured and simulated results. Measured frequency ranges from 1.25 to 40 GHz with a fractional bandwidth of 188%, BDR of 6523 and S11 < −10 dB. Even though the presented antenna operates properly over the frequency range from 1.22 to 47.5 GHz, the results of the experiment are measured till 40 GHz because of the high-frequency constraint of the existing Vector Network Analyzer (VNA). The designed SWB antenna has the benefit of good gain, concise dimension, and wide bandwidth above the formerly reported antenna structures. Simulated gain varies from 0.5 to 10.3 dBi and measured gain varies from 0.2 to 9.7 dBi. Frequency domain, as well as time-domain characterization, has been realized to guide the relevance of the proposed antenna in SWB wireless applications. Furthermore, an equivalent circuit model of the proposed antenna is developed, and the response of the circuit is obtained. The presented antenna can be employed in L, S, C, X, Ka, K, Ku, and Q band wireless communication systems.
Vacuum Ultraviolet Absorption Spectroscopy Analysis of Breath Acetone Using a Hollow Optical Fiber Gas Cell
Authors: Kudo Yudai , Kino Saiko , Matsuura Yuji
Published: January 2021

Human breath is a biomarker of body fat metabolism and can be used to diagnose various diseases, such as diabetes. As such, in this paper, a vacuum ultraviolet (VUV) spectroscopy system is proposed to measure the acetone in exhaled human breath. A strong absorption acetone peak at 195 nm is detected using a simple system consisting of a deuterium lamp source, a hollow-core fiber gas cell, and a fiber-coupled compact spectrometer corresponding to the VUV region. The hollow-core fiber functions both as a long-path and an extremely small-volume gas cell; it enables us to sensitively measure the trace components of exhaled breath. For breath analysis, we apply multiple regression analysis using the absorption spectra of oxygen, water, and acetone standard gas as explanatory variables to quantitate the concentration of acetone in breath. Based on human breath, we apply the standard addition method to obtain the measurement accuracy. The results suggest that the standard deviation is 0.074 ppm for healthy human breath with an acetone concentration of around 0.8 ppm and a precision of 0.026 ppm. We also monitor body fat burn based on breath acetone and confirm that breath acetone increases after exercise because it is a volatile byproduct of lipolysis.
Standardized Extraction Techniques for Meat Analysis with the Electronic Tongue: A Case Study of Poultry and Red Meat Adulteration
Authors: Zaukuu John-Lewis Zinia , Gillay Zoltan , Kovacs Zoltan
Published: January 2021

The electronic tongue (e-tongue) is an advanced sensor-based device capable of detecting low concentration differences in solutions. It could have unparalleled advantages for meat quality control, but the challenges of standardized meat extraction methods represent a backdrop that has led to its scanty application in the meat industry. This study aimed to determine the optimal dilution level of meat extract for e-tongue evaluations and also to develop three standardized meat extraction methods. For practicality, the developed methods were applied to detect low levels of meat adulteration using beef and pork mixtures and turkey and chicken mixtures as case studies. Dilution factor of 1% w/v of liquid meat extract was determined to be the optimum for discriminating 1% w/w, 3% w/w, 5% w/w, 10% w/w, and 20% w/w chicken in turkey and pork in beef with linear discriminant analysis accuracies (LDA) of 78.13% (recognition) and 64.73% (validation). Even higher LDA accuracies of 89.62% (recognition) and 68.77% (validation) were achieved for discriminating 1% w/w, 3% w/w, 5% w/w, 10% w/w, and 20% w/w of pork in beef. Partial least square models could predict both sets of meat mixtures with good accuracies. Extraction by cooking was the best method for discriminating meat mixtures and can be applied for meat quality evaluations with the e-tongue.
BIONOTE as an Innovative Biosensor for Measuring Endocannabinoid Levels
Authors: Grasso Simone , Santonico Marco , Pennazza Giorgio , Zompanti Alessandro , Piccoli Alessandra , Bisogno Tiziana , Maccarrone Mauro
Published: January 2021

In this study, a novel approach was developed to quantify endocannabinoids (eCBs), and was based on the liquid biosensor BIONOTE. This device is composed of a probe that can be immersed in a solution, and an electronic interface that can record a current related to the oxy-reductive reactions occurring in the sample. The two most representative members of eCBs have been analysed in vitro by BIONOTE: anandamide (N-arachidonoylethanolamine, AEA) and 2-arachidonoylglycerol (2-AG). Bovine serum albumin was used to functionalize the probe and improve the sensibility of the whole analytical system. We show that BIONOTE is able to detect both AEA and 2-AG at concentrations in the low nanomolar range, and to discriminate between these eCBs and their moieties arachidonic acid, ethanolamine and glycerol. Notably, BIONOTE distinguished these five different molecules, and it was also able to quantify AEA in human plasma. Although this is just a proof-of-concept study, we suggest BIONOTE as a cheap and user-friendly prototype sensor for high throughput quantitation of eCB content in biological matrices, with an apparent diagnostic potential for tomorrow’s medicine.
Brain Temperature Measured by Magnetic Resonance Spectroscopy to Predict Clinical Outcome in Patients with Infarction
Authors: Ishida Tomohisa , Inoue Takashi , Inoue Tomoo , Endo Toshiki , Fujimura Miki , Niizuma Kuniyasu , Endo Hidenori , Tominaga Teiji
Published: January 2021

Acute ischemic stroke is characterized by dynamic changes in metabolism and hemodynamics, which can affect brain temperature. We used proton magnetic resonance (MR) spectroscopy under everyday clinical settings to measure brain temperature in seven patients with internal carotid artery occlusion to explore the relationship between lesion temperature and clinical course. Regions of interest were selected in the infarct area and the corresponding contralateral region. Single-voxel MR spectroscopy was performed using the following parameters: 2000-ms repetition time, 144-ms echo time, and 128 excitations. Brain temperature was calculated from the chemical shift between water and N-acetyl aspartate, choline-containing compounds, or creatine phosphate. Within 48 h of onset, compared with the contralateral region temperature, brain temperature in the ischemic lesion was lower in five patients and higher in two patients. Severe brain swelling occurred subsequently in three of the five patients with lower lesion temperatures, but in neither of the two patients with higher lesion temperatures. The use of proton MR spectroscopy to measure brain temperature in patients with internal carotid artery occlusion may predict brain swelling and subsequent motor deficits, allowing for more effective early surgical intervention. Moreover, our methodology allows for MR spectroscopy to be used in everyday clinical settings.
Sensor Node Network for Remote Moisture Measurement in Timber Based on Bluetooth Low Energy and Web-Based Monitoring System
Authors: Saban Mohamed , Medus Leandro Daniel , Casans Silvia , Aghzout Otman , Rosado Alfredo
Published: January 2021

This paper proposes an IoT system based on wireless BLE connectivity to monitor the moisture content of wood, using a compact and low-cost moisture device that relies on a resistance measurement method valid for an ultra-wide range of resistance values. This device is digitally controlled with a BLE-incorporated micro-controller characterized by its small size and low power consumption, providing long-life battery. The proposed system consists of two main parts: first, the BLE moisture device including the moisture content measurement and wireless capability (BLE); second, the cloud-based monitoring platform, providing remote visualization and control for all the sensor nodes of the network. The complete infrastructure shows how multiple nodes can read and transmit moisture content of timber in buildings using small and unattended devices, with data saved in a central database and monitored by multiple commercial devices such as PC, smartphone, tablet, etc. The proposed system is innovative, scalable and low cost, and it can be deployed in wooden buildings and the wood industry, providing a practical solution that will help to avoid rot and other damaging effects caused by the moisture content.
Design and Implementation of Intelligent Agent Training Systems for Virtual Vehicles
Authors: Urrea Claudio , Garrido Felipe , Kern John
Published: January 2021

This paper presents the results of the design, simulation, and implementation of a virtual vehicle. Such a process employs the Unity videogame platform and its Machine Learning-Agents library. The virtual vehicle is implemented in Unity considering mechanisms that represent accurately the dynamics of a real automobile, such as motor torque curve, suspension system, differential, and anti-roll bar, among others. Intelligent agents are designed and implemented to drive the virtual automobile, and they are trained using imitation or reinforcement. In the former method, learning by imitation, a human expert interacts with an intelligent agent through a control interface that simulates a real vehicle; in this way, the human expert receives motion signals and has stereoscopic vision, among other capabilities. In learning by reinforcement, a reward function that stimulates the intelligent agent to exert a soft control over the virtual automobile is designed. In the training stage, the intelligent agents are introduced into a scenario that simulates a four-lane highway. In the test stage, instead, they are located in unknown roads created based on random spline curves. Finally, graphs of the telemetric variables are presented, which are obtained from the automobile dynamics when the vehicle is controlled by the intelligent agents and their human counterpart, both in the training and the test track.
Strain Transfer Characteristics of Multi-Layer Optical Fiber Sensors with Temperature-Dependent Properties at Low Temperature
Authors: Yang Taolue , Wang Huaping , Wang Xingzhe
Published: January 2021

Optical fiber sensors have been potentially expected to apply in the extreme environment for their advantages of measurement in a large temperature range. The packaging measure which makes the strain sensing fiber survive in these harsh conditions will commonly introduce inevitable strain transfer errors. In this paper, the strain transfer characteristics of a multi-layer optical fiber sensing structure working at cryogenic environment with temperature gradients have been investigated theoretically. A generalized three-layer shear lag model incorporating with temperature-dependent properties of layers was developed. The strain transfer relationship between the optical fiber core and the matrix has been derived in form of a second-order ordinary differential equation (ODE) with variable coefficients, where the Young’s modulus and the coefficients of thermal expansion (CTE) are considered as functions of temperature. The strain transfer characteristics of the optical sensing structure were captured by solving the ODE boundary problems for cryogenic temperature loads. Case studies of the cooling process from room temperature to some certain low temperatures and gradient temperature loads for different low-temperature zones were addressed. The results showed that different temperature load configurations cause different strain transfer error features which can be described by the proposed model. The protective layer always plays a main role, and the optimization geometrical parameters should be carefully designed. To verify the theoretical predictions, an experiment study on the thermal strain measurement of an aluminum bar with optical fiber sensors was conducted. LUNA ODiSI 6100 integrator was used to measure the Rayleigh backscattering spectra shift of the optical fiber at a uniform temperature and a gradient temperature under liquid nitrogen temperature zone, and a reasonable agreement with the theory was presented.