bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic Recrystallization of Olivine During Simple Shear: Evolution of Microstructure and Crystallographic Preferred Orientation From Full‐Field Numerical Simulations
Authors: Yu Y. , Griera A. , Gomez‐Rivas E. , Bons P. D. , García‐Castellanos D. , Hao B. , Lebensohn R. A. , Llorens M.‐G.
Published: September 2024

Upper mantle deformation is mainly controlled by the mechanical behavior of olivine. Crystallographic preferred orientations (CPOs) develop in olivine due to crystal‐plastic deformation during mantle flow, where the a‐axes of olivine polycrystalline aggregates are aligned with the flow direction. Therefore, the observed CPO in olivine‐rich rocks is used as an indicator of the mantle flow direction. Experimental data show that olivine rheology is strongly controlled by the microstructure. While the influence of plastic deformation is in general well characterized, the role of dynamic recrystallization during deformation is not totally understood, limiting our ability to interpret the deformation history of naturally deformed rocks. This contribution presents microdynamic numerical simulations of olivine polycrystalline aggregates with different iron content (i.e., fayalite content) with the aim of exploring the CPO and grain size response to dynamic recrystallization. We use a full‐field approach with an explicit simulation of viscoplastic deformation and dynamic recrystallization processes under simple shear boundary conditions up to high strain. The simulations show that the CPOs are similar and practically reach the same maximum regardless of the iron content. CPOs are characterized by a single cluster of a‐axis and two‐clusters of b‐axis, reveling a joint activity of the easy glide [100](010) and the moderate strength [100](010) slip systems. High‐strain domains of our models are consistent with experimental results, showing an A‐type fabric with double maxima, and where the CPO is aligned with the shear direction. The model provides a deeper understanding of the dynamic recrystallization influence on olivine CPOs resulting from plastic deformation.
Reduction of the Tropical Atmospheric Dynamics Into Shallow‐Water Analogs: A Formulation Analysis
Authors: Yano Jun‐Ichi
Published: September 2024

The shallow‐water analogue models for the tropical atmosphere are examined from a formulational point of view. The normal‐mode approach provides a formal procedure to reduce the primitive equation system to a shallow‐water analogue, although approaches based on vertical integrals of the primitive equation system may be more intuitively appealing. Under a general framework of the latter, classical models by Gill (1980, https://doi.org/10.1256/smsqj.44904) and Lindzen and Nigam (1987, 2.0.co;2>https://doi.org/10.1175/1520‐0469(1987)044<2418:otross>2.0.co;2) are derived in a deductive manner, by elucidating their limitations, implications, as well physical processes assumed. Major advantage of shallow‐water analogue models is that after a vertical integral, the determination of convective heating rate simply reduces to that of a precipitation rate. Consequently, the question of representing convection also almost reduces to that of precipitation. This fact leads to confusions in literature about distinction between large‐scale precipitation and subgrid‐scale convection. This framework further supports a popular notion of the moisture as a key variable for describing convection. By reviewing the existing formulations, it is shown that convection can be parameterized without moisture under the limit of the parcel‐environment quasi‐equilibrium.
In‐Stream Nitrogen Dynamics in a Point Source Influenced Headwater Stream During Baseflow Conditions
Authors: Spill Caroline , Ditzel Lukas , Gassmann Matthias
Published: September 2024

Hydrochemical signatures are often traced back to their original sources using data collected at catchment outlets. However, this approach introduces uncertainties, as signals may add up, cancel each other out, or be subject to transformation processes. Specifically rural point sources, such as communal wastewater treatment plants (WWTPs), are often overlooked and remain poorly understood in terms of their (local) impact, on water quality and quantity dynamics. We equipped a point source‐influenced headwater catchment with a comprehensive measurement setup, to directly trace the different hydrochemical signals. Statistical approaches were used to address c‐Q relationships and hydrochemical drivers for nutrient export upstream, downstream and within the WWTP during baseflow conditions. Groundwater infiltration into the old and leaky sewer system as well as rainwater collected via the combined sewer system were found to significantly alter processes within the WWTP, resulting in highly variable effluent nutrient concentrations. Ammonium introduced by the WWTP is rapidly transformed in the stream, leading to increasing nitrate concentrations further downstream. The combination of processes introduced by the WWTP overlap the dilution and (non‐significant) chemostatic patterns of the upstream nitrate‐discharge relationship, leading to enrichment patterns shortly after, and mainly diluting patterns 290 m downstream of the WWTP. Regarding maximum nutrient concentrations, dry periods during autumn were particularly critical, as the WWTP introduced high ammonium concentrations, which coincided with high nitrate concentrations from the catchment and a minimal dilution potential of the stream. Our study demonstrates the importance of incorporating all nutrient sources into catchment analyses, to facilitate successful management decisions.
Deriving Flow Velocity and Initial Concentration From Liesegang‐Like Patterns
Authors: Liu Chong , Calo Victor M. , Regenauer‐Lieb Klaus , Hu Manman
Published: September 2024

Zebra rocks, characterized by their striking reddish‐brown stripes, rods, and spots of hematite (Fe‐oxide), showcase complex self‐organized patterns formed under far‐from‐equilibrium conditions. Despite their ease of recognition, the underlying mechanisms of pattern‐forming processes remain elusive. We introduce a novel advection‐dominated phase‐field model that effectively replicates the Liesegang‐like patterns observed in Zebra rocks. This numerical model leverages the concept of phase separation, a well‐established principle governing Liesegang phenomena in a two‐dimensional setting. Our findings reveal that initial solute concentration and fluid flow velocity are critical determinants in pattern morphologies. We quantitatively explain the spacing and width of a specific Liesegang‐like pattern category. Furthermore, the model demonstrates that vanishingly low initial concentrations promote the formation of oblique patterns, with inclination angles influenced by rock heterogeneity. Additionally, we establish a quantitative relationship between band thickness and geological parameters for orthogonal bands. This enables the characterization of critical geological parameters based solely on static patterns observed in Zebra rocks, providing valuable insights into their formation environments. The diverse patterns in Zebra rocks share similarities with morphologies observed on early Earth and Mars, such as banded iron formations and hematite spherules. Our model, therefore, offers a plausible explanation for the formation mechanisms of these patterns and presents a powerful tool for deciphering the geochemical environments of their origin.
Climate, Hydrology, and Nutrients Control the Seasonality of Si Concentrations in Rivers
Authors: Johnson Keira , Jankowski Kathi Jo , Carey Joanna C. , Sethna Lienne R. , Bush Sidney A. , McKnight Diane , McDowell William H. , Wymore Adam S. , Kortelainen Pirkko , Jones Jeremy B. , Lyon Nicholas J. , Laudon Hjalmar , Poste Amanda E. , Sullivan Pamela L.
Published: September 2024

The seasonal behavior of fluvial dissolved silica (DSi) concentrations, termed DSi regime, mediates the timing of DSi delivery to downstream waters and thus governs river biogeochemical function and aquatic community condition. Previous work identified five distinct DSi regimes across rivers spanning the Northern Hemisphere, with many rivers exhibiting multiple DSi regimes over time. Several potential drivers of DSi regime behavior have been identified at small scales, including climate, land cover, and lithology, and yet the large‐scale spatiotemporal controls on DSi regimes have not been identified. We evaluate the role of environmental variables on the behavior of DSi regimes in nearly 200 rivers across the Northern Hemisphere using random forest models. Our models aim to elucidate the controls that give rise to (a) average DSi regime behavior, (b) interannual variability in DSi regime behavior (i.e., Annual DSi regime), and (c) controls on DSi regime shape (i.e., minimum and maximum DSi concentrations). Average DSi regime behavior across the period of record was classified accurately 59% of the time, whereas Annual DSi regime behavior was classified accurately 80% of the time. Climate and primary productivity variables were important in predicting Average DSi regime behavior, whereas climate and hydrologic variables were important in predicting Annual DSi regime behavior. Median nitrogen and phosphorus concentrations were important drivers of minimum and maximum DSi concentrations, indicating that these macronutrients may be important for seasonal DSi drawdown and rebound. Our findings demonstrate that fluctuations in climate, hydrology, and nutrient availability of rivers shape the temporal availability of fluvial DSi.
Gestational urinary concentrations of glyphosate and aminomethylphosphonic acid in relation to preterm birth: the MIREC study
Authors: Ashley-Martin Jillian , Marro Leonora , Owen James , Borghese Michael M. , Arbuckle Tye , Bouchard Maryse F. , Lanphear Bruce , Walker Mark , Foster Warren , Fisher Mandy
Published: September 2024

Background

Few high-quality studies have evaluated associations between urinary glyphosate or its environmental degradate (aminomethylphosphonic acid (AMPA)] and preterm birth (PTB).

Objectives

To quantify associations between urinary glyphosate and AMPA and preterm birth in the pan-Canadian Maternal-Infant Research on Environmental Chemicals (MIREC) study and determine if associations differ by fetal sex.

Methods

We measured first trimester urinary glyphosate and AMPA concentrations in MIREC participants who were recruited between 2008–2011 from 10 Canadian cities. Of the 1880 participants whose first trimester urine samples were analyzed for glyphosate or AMPA, 1765 delivered a singleton, live birth. Our primary outcome was preterm birth (PTB) defined as births occurring between 20 and <37 weeks. Secondary outcomes were spontaneous preterm births (sPTB) and gestational age. We modelled the hazard of PTB and sPTB using discrete time survival analysis with multivariable logistic regression to calculate odds ratios (OR). We used multivariable linear regression models to quantify associations between analytes and gestational age. To assess effect modification by fetal sex, we stratified all models and calculated interaction terms. In the logistic regressions models we additionally calculated the relative excess risk due to interaction.

Results

Six percent (n = 106) of the study population delivered preterm, and 4.7% (n = 83) had a spontaneous preterm birth. Median specific-gravity standardized concentrations of glyphosate and AMPA were 0.25 and 0.21 µg/L. Associations between both glyphosate or AMPA and PTB, sPTB, and gestational age centered around the null value. The adjusted ORs of PTB for each doubling of glyphosate and AMPA concentrations were 0.98 (95% CI: 0.94, 1.03) and 0.99 (95% CI: 0.92, 1.06) respectively. We observed no evidence of differences by fetal sex.

Conclusions

In this Canadian pregnancy cohort, neither glyphosate nor AMPA urinary concentrations was associated with PTB or reduced gestational length.
Impaired emotion recognition in Cntnap2-deficient mice is associated with hyper-synchronous prefrontal cortex neuronal activity
Authors: Mohapatra Alok Nath , Jabarin Renad , Ray Natali , Netser Shai , Wagner Shlomo
Published: September 2024

Individuals diagnosed with autism spectrum disorder (ASD) show difficulty in recognizing emotions in others, a process termed emotion recognition. While human fMRI studies linked multiple brain areas to emotion recognition, the specific mechanisms underlying impaired emotion recognition in ASD are not clear. Here, we employed an emotional state preference (ESP) task to show that Cntnap2-knockout (KO) mice, an established ASD model, do not distinguish between conspecifics according to their emotional state. We assessed brain-wide local-field potential (LFP) signals during various social behavior tasks and found that Cntnap2-KO mice exhibited higher LFP theta and gamma rhythmicity than did C57BL/6J mice, even at rest. Specifically, Cntnap2-KO mice showed increased theta coherence, especially between the prelimbic cortex (PrL) and the hypothalamic paraventricular nucleus, during social behavior. Moreover, we observed significantly increased Granger causality of theta rhythmicity between these two brain areas, across several types of social behavior tasks. Finally, optogenetic stimulation of PrL pyramidal neurons in C57BL/6J mice impaired their social discrimination abilities, including in ESP. Together, these results suggest that increased rhythmicity of PrL pyramidal neuronal activity and its hyper-synchronization with specific brain regions are involved in the impaired emotion recognition exhibited by Cntnap2-KO mice.
Antibiotic exposure for culture-negative early-onset sepsis in late-preterm and term newborns: an international study
Authors: Dimopoulou Varvara , Klingenberg Claus , Navér Lars , Nordberg Viveka , Berardi Alberto , el Helou Salhab , Fusch Gerhard , Bliss Joseph M. , Lehnick Dirk , Guerina Nicholas , Seliga-Siwecka Joanna , Maton Pierre , Lagae Donatienne , Mari Judit , Janota Jan , Agyeman Philipp K. A. , Pfister Riccardo , Latorre Giuseppe , Maffei Gianfranco , Laforgia Nicola , Mózes Enikő , Størdal Ketil , Strunk Tobias , Stocker Martin , Giannoni Eric ,   the AENEAS Study Group, Grazia Capretti Maria , Martina Ceccoli , Morena De Angelis , Pietro Drimaco , Khalyane Eap , Zoe el Helou , Rana Esmaeilizand , Alessandra Foglianese , Carmelo Geraci , Bartłomiej Grochowski , Stellan Håkansson , Sharandeep Kaur , Anne-Louise Kollegger , Frida Oldendorff , Vittoria Rizzo , Rønnestad Arild E. , Damber Shrestha , Jørgen Stensvold Hans , Martin Trefny , Kristyna Zilinska , Aleksandra Zwijacz
Published: September 2024

Background

Early-life antibiotic exposure is disproportionately high compared to the burden of culture-proven early-onset sepsis (CP-EOS). We assessed the contribution of culture-negative cases to the overall antibiotic exposure in the first postnatal week.

Methods

We conducted a retrospective analysis across eleven countries in Europe, North America, and Australia. All late-preterm and term infants born between 2014 and 2018 who received intravenous antibiotics during the first postnatal week were classified as culture-negative cases treated for ≥5 days (CN ≥ 5d), culture-negative cases treated for <5 days (CN < 5d), or CP-EOS cases.

Results

Out of 757,979 infants, 21,703 (2.9%) received intravenous antibiotics. The number of infants classified as CN ≥ 5d, CN < 5d, and CP-EOS was 7996 (37%), 13,330 (61%), and 375 (1.7%). The incidence of CN ≥ 5d, CN < 5d, and CP-EOS was 10.6 (95% CI 10.3–10.8), 17.6 (95% CI 17.3–17.9), and 0.49 (95% CI 0.44–0.54) cases per 1000 livebirths. The median (IQR) number of antibiotic days administered for CN ≥ 5d, CN < 5d, and CP-EOS was 77 (77–78), 53 (52–53), and 5 (5-5) per 1000 livebirths.

Conclusions

CN ≥ 5d substantially contributed to the overall antibiotic exposure, and was 21-fold more frequent than CP-EOS. Antimicrobial stewardship programs should focus on shortening antibiotic treatment for culture-negative cases.

Impact

In a study of 757,979 infants born in high-income countries, we report a presumed culture-negative early-onset sepsis incidence of 10.6/1000 livebirths with an associated antibiotic exposure of 77 antibiotic days per 1000 livebirths.

This study sheds light on the major contribution of presumed culture-negative early-onset sepsis to early-life antibiotic exposure.

Given the diagnostic uncertainty surrounding culture-negative early-onset sepsis, the low mortality rate, and the disproportionate antibiotic exposure associated with this condition, our study emphasizes the importance of targeting culture-negative early-onset sepsis in antimicrobial stewardship programs.
Elevated cerebral perfusion in neonatal encephalopathy is associated with neurodevelopmental impairments
Authors: Tuura Ruth O’Gorman , Kottke Raimund , Brotschi Barbara , Sabandal Carola , Hagmann Cornelia , Latal Beatrice
Published: September 2024

Background

Neonatal encephalopathy (NE) represents a primary cause of neonatal death and neurodevelopmental impairments. In newborns with NE, cerebral hyperperfusion is related to an increased risk of severe adverse outcomes, but less is known about the link between perfusion and mild to moderate developmental impairments or developmental delay.

Methods

Using arterial spin labelling perfusion MRI, we investigated the link between perfusion in 36 newborns with NE and developmental outcome at 2 years.

Results

53% of the infants demonstrated a normal outcome at 24 months, while two had cerebral palsy with impairments in cognitive, motor, and language domains, and three infants died. The remaining infants showed mild or moderate delays in development in one or two domains. Hyperperfusion across the whole brain was associated with more adverse outcome, including an increased risk of death or severe disability such as cerebral palsy. Among the surviving infants, higher perfusion in the bilateral basal ganglia, thalamus, hippocampus and cerebellum during the neonatal period was related to a poorer cognitive outcome at 2 years.

Conclusion

Hyperperfusion in infants with NE was associated with a more adverse outcome and lower cognitive outcome scores. In addition to severe adverse outcomes, altered perfusion is also related to mild to moderate impairment following HIE.

Impact statement

Neonates with neonatal encephalopathy (NE) show increased cerebral perfusion globally, which is linked to a more adverse outcome.

Higher perfusion in the bilateral basal ganglia, thalamus, hippocampus and cerebellum during the neonatal period was related to a poorer cognitive outcome at 2 years.

In addition to severe adverse outcomes altered perfusion is related to mild to moderate impairment following NE.

To improve neurodevelopmental outcomes, it is important to improve our understanding of the factors influencing cerebral perfusion in infants with NE.
Recommended procedures for managing carious lesions in primary teeth with pulp involvement—a scoping review
Authors: Maldupa Ilze , Al-Yaseen Waraf , Giese Julius , Ahmed Elagami Rokaia , Raggio Daniela Prócida
Published: September 2024

Background

Managing dental caries in primary teeth with pulp involvement is a significant challenge. Clinical guidelines offer recommendations for effective management.

Aim

To identify and analyze policies, guidelines, and recommendations for treating primary teeth with pulp-involved carious lesions, highlighting existing research gaps and setting the foundation for future research.

Methods

A comprehensive search was conducted across databases (PubMed, Scopus, Embase, GIN, and LILACS) and grey literature sources (Trip and ProQuest) to identify guidelines, consensus, policy, and position statements on primary teeth pulp therapy and extraction thresholds. Two independent reviewers screened the abstracts and titles, followed by full-text screening.

Results

After removing duplication, of the 1098 records, 14 were selected for analysis. This review examined various treatments for deep caries lesions in primary teeth, including indirect/direct pulp capping, pulpotomy, pulpectomy, lesion sterilization/tissue restoration, and extraction. Time search was restricted to documents published from 30th January 2008 to 30th January 2024, offering insights into evolving clinical practices.

Conclusion

Treatment for carious lesions in primary teeth involving the pulp depends on clinical indications and may involve minimally invasive techniques. Recommended options are indirect pulp capping, pulpotomy, and pulpectomy, while direct capping and tooth removal are discouraged. Further research is needed to address gaps, improve guideline development, and enhance consistency of recommendations.
Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3tm1Flv mice
Authors: Weiss Felix D. , Alvarez Yubell , Shakeri Farhad , Sahu Anshupa , Leka Petro , Dernst Alesja , Rollheiser Jessika , Vasconcelos Matilde , Geraci Adriana , Duthie Fraser , Stahl Rainer , Lee Hye Eun , Gellner Anne-Kathrin , Buness Andreas , Latz Eicke , Meissner Felix
Published: September 2024

Immune response genes are highly polymorphic in humans and mice, with heterogeneity amongst loci driving strain-specific host defence responses. The inadvertent retention of polymorphic loci can introduce confounding phenotypes, leading to erroneous conclusions, and impeding scientific advancement. In this study, we employ a combination of RNAseq and variant calling analyses to identify a substantial region of 129S genome, including the highly polymorphic Nlrp1 locus, proximal to Nlrp3, in one of the most commonly used mouse models of NLRP3 deficiency (Nlrp3tm1Flv). We show that the presence of the Nlrp1129S locus leads to an increase in NLRP1B protein expression, and a sensitising of Nlrp3tm1Flv macrophages to NLRP1 inflammasome activation, independent of NLRP3 deficiency. Retention of 129S genome further leads to protein sequence differences and altered gene regulation across multiple cell types, including of the key tissue-resident macrophage marker, TIM4. Using alternative models of NLRP3 deficiency, including a previously undescribed conditional Nlrp3 allele enabling precise temporal and cell-type specific control over Nlrp3 deletion, we further show that NLRP3 contributes to Talabostat-driven IL-1β release. Our study also establishes a generic framework to identify functionally relevant SNPs and assess genomic contamination in transgenic mice using RNAseq data. This allows for unambiguous attribution of phenotypes to the target gene and advances the precision and reliability of research in the field of host defence responses.
Iron chelation as a new therapeutic approach to prevent senescence and liver fibrosis progression
Authors: Amengual Josep , Alay Ania , Vaquero Javier , Gonzalez-Sanchez Ester , Bertran Esther , Sánchez Aránzazu , Herrera Blanca , Meyer Kathleen , Maus Mate , Serrano Manuel , Martínez-Chantar María Luz , Fabregat Isabel
Published: September 2024

Iron overload and cellular senescence have been implicated in liver fibrosis, but their possible mechanistic connection has not been explored. To address this, we have delved into the role of iron and senescence in an experimental model of chronic liver injury, analyzing whether an iron chelator would prevent liver fibrosis by decreasing hepatocyte senescence. The model of carbon tetrachloride (CCl4) in mice was used as an experimental model of liver fibrosis. Results demonstrated that during the progression of liver fibrosis, accumulation of iron occurs, concomitant with the appearance of fibrotic areas and cells undergoing senescence. Isolated parenchymal hepatocytes from CCl4-treated mice present a gene transcriptomic signature compatible with iron accumulation and senescence, which correlates with induction of Reactive Oxygen Species (ROS)-related genes, activation of the Transforming Growth Factor-beta (TGF-β) pathway and inhibition of oxidative metabolism. Analysis of the iron-related gene signature in a published single-cell RNA-seq dataset from CCl4-treated livers showed iron accumulation correlating with senescence in other non-parenchymal liver cells. Treatment with deferiprone, an iron chelator, attenuated iron accumulation, fibrosis and senescence, concomitant with relevant changes in the senescent-associated secretome (SASP), which switched toward a more anti-inflammatory profile of cytokines. In vitro experiments in human hepatocyte HH4 cells demonstrated that iron accumulates in response to a senescence-inducing reagent, doxorubicin, being deferiprone able to prevent senescence and SASP, attenuating growth arrest and cell death. However, deferiprone did not significantly affect senescence induced by two different agents (doxorubicin and deoxycholic acid) or activation markers in human hepatic stellate LX-2 cells. Transcriptomic data from patients with different etiologies demonstrated the relevance of iron accumulation in the progression of liver chronic damage and fibrosis, correlating with a SASP-related gene signature and pivotal hallmarks of fibrotic changes. Altogether, our study establishes iron accumulation as a clinically exploitable driver to attenuate pathological senescence in hepatocytes.
Enhancers in T Cell development and malignant lesions
Authors: Zhang Tong , Zou Lin
Published: September 2024

Enhancers constitute a vital category of cis-regulatory elements with a Mediator complex within DNA sequences, orchestrating gene expression by activating promoters. In the development of T cells, some enhancers regulate the critical genes, which might also regulate T cell malignant lesions. This review is to comprehensively elucidate the contributions of enhancers in both normal T cell development and its malignant pathogenesis, proposing the idea that the precise subunits of the Mediator complex are the potential drug target for disrupting the specific gene enhancer for T cell malignant diseases.
in situ observation of reversible phase transitions in Gd-doped ceria driven by electron beam irradiation
Authors: Ran Ke , Zeng Fanlin , Jin Lei , Baumann Stefan , Meulenberg Wilhelm A. , Mayer Joachim
Published: September 2024

Ceria-based oxides are widely utilized in diverse energy-related applications, with attractive functionalities arising from a defective structure due to the formation of mobile oxygen vacancies ($${V}_{O}^{\cdot \cdot }$$

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). Notwithstanding its significance, behaviors of the defective structure and $${V}_{O}^{\cdot \cdot }$$

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in response to external stimuli remain incompletely explored. Taking the Gd-doped ceria (Ce0.88Gd0.12O2-δ) as a model system and leveraging state-of-the-art transmission electron microscopy techniques, reversible phase transitions associated with massive $${V}_{O}^{\cdot \cdot }$$

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rearrangement are stimulated and visualized in situ with sub-Å resolution. Electron dose rate is identified as a pivotal factor in modulating the phase transition, and both the $${V}_{O}^{\cdot \cdot }$$

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concentration and the orientation of the newly formed phase can be altered via electron beam. Our results provide indispensable insights for understanding and refining the microscopic pathways of phase transition as well as defect engineering, and could be applied to other similar functional oxides.
Understanding the origin of lithium dendrite branching in Li6.5La3Zr1.5Ta0.5O12 solid-state electrolyte via microscopy measurements
Authors: Yildirim Can , Flatscher Florian , Ganschow Steffen , Lassnig Alice , Gammer Christoph , Todt Juraj , Keckes Jozef , Rettenwander Daniel
Published: September 2024

Lithium dendrite growth in inorganic solid-state electrolytes acts as a main stumbling block for the commercial development of all-solid-state lithium batteries. Indeed, Li dendrites often lead to solid-state electrolyte fractures, undermining device integrity and safety. Despite the significance of these issues, the mechanisms driving the solid-state electrolyte fracture process at the microscopic level remain poorly understood. Here, via operando optical and ex situ dark field X-ray microscopy measurements of LiSn∣single-crystal Li6.5La3Zr1.5Ta0.5O12∣LiSn symmetric cells, we provide insights into solid-state electrolyte strain patterns and lattice orientation changes associated with dendrite growth. We report the observation of dislocations in the immediate vicinity of dendrite tips, including one instance where a dislocation is anchored directly to a tip. This latter occurrence in single-crystalline ceramics suggests an interplay between dendrite proliferation and dislocation formation. We speculate that the mechanical stress induced by dendrite expansion triggers dislocation generation. These dislocations seem to influence the fracture process, potentially affecting the directional growth and branching observed in lithium dendrites.
Frontoparietal network topology as a neural marker of musical perceptual abilities
Authors: Lumaca M. , Keller P. E. , Baggio G. , Pando-Naude V. , Bajada C. J. , Martinez M. A. , Hansen J. H. , Ravignani A. , Joe N. , Vuust P. , Vulić K. , Sandberg K.
Published: September 2024

Why are some individuals more musical than others? Neither cognitive testing nor classical localizationist neuroscience alone can provide a complete answer. Here, we test how the interplay of brain network organization and cognitive function delivers graded perceptual abilities in a distinctively human capacity. We analyze multimodal magnetic resonance imaging, cognitive, and behavioral data from 200+ participants, focusing on a canonical working memory network encompassing prefrontal and posterior parietal regions. Using graph theory, we examine structural and functional frontoparietal network organization in relation to assessments of musical aptitude and experience. Results reveal a positive correlation between perceptual abilities and the integration efficiency of key frontoparietal regions. The linkage between functional networks and musical abilities is mediated by working memory processes, whereas structural networks influence these abilities through sensory integration. Our work lays the foundation for future investigations into the neurobiological roots of individual differences in musicality.
Tracing the electron transport behavior in quantum-dot light-emitting diodes via single photon counting technique
Authors: Su Qiang , Chen Zinan , Chen Shuming
Published: September 2024

The electron injection and transport behavior are of vital importance to the performance of quantum-dot light-emitting diodes. By simultaneously measuring the electroluminescence-photoluminescence of the quantum-dot light-emitting diodes, we identify the presence of leakage electrons which leads to the discrepancy of the electroluminescence and the photoluminescence roll-off. To trace the transport paths of the leakage electrons, a single photon counting technique is developed. This technique enables us to detect the weak photon signals and thus provides a means to visualize the electron transport paths at different voltages. The results show that, the electrons, except those recombining within the quantum-dots, leak to the hole transport layer or recombine at the hole transport layer/quantum-dot interface, thus leading to the reduction of efficiency. By reducing the amount of leakage electrons, quantum-dot light-emitting diode with an internal power conversion efficiency of over 98% can be achieved.
Transparency and adaptability aid in realigning the complexity of objectives, approaches, and systems in human-wildlife coexistence research
Authors: Hoffmann Claire F. , Beck Jacalyn M. , Kaihula Roselyn W. , Montgomery Robert A.
Published: September 2024

Human-wildlife interactions are situated within dynamic systems, characterized by social and ecological complexity. Human-wildlife coexistence research, however, typically focuses on one component of these systems in isolation. We inadvertently followed this norm while carrying out semi-structured interviews of livestock-owners in Northern Tanzania. As existing literature highlighted that this area was a hotspot for livestock depredation, our research questions focused on human interactions with carnivores. Interestingly, almost three quarters (72%, n = 72 of 100) of study participants independently raised African elephants (Loxodonta africana) as presenting the greatest impediments to coexistence. By centering our interviews on carnivores, we omitted vital components of this complex system. To counteract the effects of this oversimplification, we changed our intended analytical process after data collection. Instead of conducting a quantitative analysis of rates of livestock depredation and perceptions of risk posed by a suite of sympatric carnivores, we applied a grounded theory approach to assess interactions across multiple dimensions of this complex system. Through this transparent effort to realign our approaches with the complexity of the study system, we highlight the importance of designing research approaches that effectively reflect the complexities inherent to human-wildlife coexistence.
How storage post sampling influences the stability of sebum when used for mass spectrometry metabolomics analysis?
Authors: Walton-Doyle Caitlin , Sinclair Eleanor , Begum Humayra , Hollywood Katherine A. , Trivedi Drupad K. , Barran Perdita
Published: September 2024

Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson’s Disease. Given that sebum sampling is facile and non-invasive, its potential for use in clinical biochemistry diagnostic assays should be explored including the parameters for standard operating procedures around collection, transport, and storage. To this aim we have here investigated the reproducibility of mass spectrometry data from sebum in relation to both storage temperature and length of storage. Sebum samples were collected from volunteers and stored for up to four weeks at a range of temperatures: ambient (circa 17 °C), −20 °C and −80 °C. Established extraction protocols were employed and samples were analysed by two chromatographic mass spectrometry techniques and data investigated using PCA, PLS-DA and ANOVA. We cannot discriminate samples as a function of storage temperature or time stored in unsupervised analysis using data acquired via TD–GC–MS and LC–IM–MS, although the sampling of volatiles was susceptible to batch effects. This study indicates that the requirements for storage and transport of sebum samples that may be used in clinical assays are less stringent than for liquid samples and indicate that sebum is suitable for remote and at home sampling prior to analysis.
Improved oxidation behavior of Hf0.11Al0.20B0.69 in comparison to Hf0.28B0.72 magnetron sputtered thin films
Authors: Kümmerl Pauline , Lellig Sebastian , Navidi Kashani Amir Hossein , Hans Marcus , Pöllmann Peter J. , Löfler Lukas , Nayak Ganesh Kumar , Holzapfel Damian M. , Kolozsvári Szilárd , Polcik Peter , Schweizer Peter , Primetzhofer Daniel , Michler Johann , Schneider Jochen M.
Published: September 2024

The oxidation resistance of Hf0.28B0.72 and Hf0.11Al0.20B0.69 thin films was investigated comparatively at 700 °C for up to 8 h. Single-phase solid solution thin films were co-sputtered from HfB2 and AlB2 compound targets. After oxidation at 700 °C for 8 h an oxide scale thickness of 31 $$\pm$$
±
 2 nm was formed on Hf0.11Al0.20B0.69 which corresponds to 14% of the scale thickness measured on Hf0.28B0.72. The improved oxidation resistance can be rationalized based on the chemical composition and the morphology of the formed oxide scales. On Hf0.28B0.72 the formation of a porous, O, Hf, and B-containing scale and the formation of crystalline HfO2 is observed. Whereas on Hf0.11Al0.20B0.69 a dense, primarily amorphous scale containing O, Al, B as well as approximately 3 at% of Hf forms, which reduces the oxidation kinetics significantly by passivation. Benchmarking Hf0.11Al0.20B0.69 with Ti–Al-based boride and nitride thin films with similar Al concentrations reveals superior oxidation behavior of the Hf-Al-based thin film. The incorporation of few at% of Hf in the oxide scale decelerates oxidation kinetics at 700 °C and leads to a reduction in oxide scale thickness of 21% and 47% compared to Ti0.12Al0.21B0.67 and Ti0.27Al0.21N0.52, respectively. Contrary to Ti–Al-based diborides, Hf0.11Al0.20B0.69 shows excellent oxidation behavior despite B-richness.
Exosomal fragment enclosed polyamine-salt nano-complex for co-delivery of docetaxel and mir-34a exhibits higher cytotoxicity and apoptosis in breast cancer cells
Authors: Basak Moumita , Kulkarni Mrunal , Narisepalli Saibhargav , Chitkara Deepak , Mittal Anupama
Published: September 2024

A novel core–shell nanocarrier system has been designed for co-delivery of a small anticancer drug, docetaxel (DTX) and tumor suppressor (TS) miR-34a named as Exo(PAN34a+DTX). The core is formed by pH dependent polyamine salt aggregates (PSA) containing both the payloads and the shell is formed by RAW 264.7 cell derived exosomal fragments. Herein, phosphate driven polyallylamine hydrochloride (PAH, MW:17,500 Da) PSA was formed in presence of miR-34a and DTX to form PAN34a+DTX. The formulation exhibited pH dependent DTX release with only 33.55 ± 2.12% DTX release at pH 7.2 and 75.21 ± 1.8% DTX release till 144 h at pH 5.5. At 1.21 molar ratio of phosphate to the amine (known as R value), efficient complexation of miR-34a (3.6 μM) in the PAN particles was obtained. PAN34a+DTX demonstrated particle size (163.86 ± 12.89 nm) and zeta-potential value of 17.53 ± 5.10 mV which upon exosomal fragment layering changed to − 7.23 ± 2.75 mV which is similar to the zeta-potential of the exosomal fragments, i.e., − 8.40 ± 1.79 mV. The final formulation Exo(PAN34a+DTX), loaded with 40 ng/mL DTX and 50 nM miR-34a exhibited 48.20 ± 4.59% cytotoxicity in triple negative breast cancer (TNBC) cells, 4T1. Co-localization of CM-DiI (red fluorescence) stained exosomal fragments and FAM-siRNA (green fluorescence) in the cytoplasm of 4T1 cells after 6 h of Exo(PANFAM) treatment confirmed the efficiency of the designed system to co-deliver two actives. Exo(PAN34a+DTX) also reduced BCL-2 expression (target gene for miR-34a) by 8.98 folds in comparison to free DTX confirming promising co-delivery and apoptosis inducing effect of Exo(PAN34a+DTX) in 4T1.
Predator exposure early in life shapes behavioral development and individual variation in a clonal fish
Authors: Scherer U. , Laskowski K. L. , Kressler M. M. , Ehlman S. M. , Wolf M. , Bierbach D.
Published: September 2024

Predation risk is one of the most important factors generating behavioral differences among populations. In addition, recent attention focusses on predation as a potential driver of patterns of individual behavioral variation within prey populations. Previous studies provide mixed results, reporting either increased or decreased among-individual variation in response to risk. Here, we take an explicit developmental approach to documenting how among-individual variation develops over time in response to predator exposure, controlling for both genetic and experiential differences among individuals. We reared juveniles of naturally clonal Amazon mollies, Poecilia formosa, either with or without a predator visible during feedings over 4 weeks and analyzed activity during feedings, time spent feeding and number of visits to the feeding spot. (I) Predator-exposed fish did not differ from control fish in average feeding behavior, but they were less active during feeding trials. (II) In the absence of the predator, substantial changes in among-individual variation over time were detected: among-individual differences in feeding duration increased whereas differences in activity decreased, but there were no changes in feeder visits. In contrast, in the presence of a predator, among-individual variation in all three behaviors was stable over time and often lower compared to control conditions. Our work suggests that predation risk may have an overall stabilizing effect on the development of individual variation and that differences in predation risk may well lead to population-wide differences in among-individual behavioral variation.
Screening of the Pandemic Response Box library identified promising compound candidate drug combinations against extensively drug-resistant Acinetobacter baumannii
Authors: Boonyalai Nonlawat , Peerapongpaisarn Dutsadee , Thamnurak Chatchadaporn , Oransathid Wilawan , Wongpatcharamongkol Nantanat , Oransathid Wirote , Lurchachaiwong Woradee , Griesenbeck John S. , Waters Norman C. , Demons Samandra T. , Ruamsap Nattaya , Vesely Brian A.
Published: September 2024

Infections caused by antimicrobial-resistant Acinetobacter baumannii pose a significant threat to human health, particularly in the context of hospital-acquired infections. As existing antibiotics lose efficacy against Acinetobacter isolates, there is an urgent need for the development of novel antimicrobial agents. In this study, we assessed 400 structurally diverse compounds from the Medicines for Malaria Pandemic Response Box for their activity against two clinical isolates of A. baumannii: A. baumannii 5075, known for its extensive drug resistance, and A. baumannii QS17-1084, obtained from an infected wound in a Thai patient. Among the compounds tested, seven from the Pathogen box exhibited inhibitory effects on the in vitro growth of A. baumannii isolates, with IC50s ≤ 48 µM for A. baumannii QS17-1084 and IC50s ≤ 17 µM for A. baumannii 5075. Notably, two of these compounds, MUT056399 and MMV1580854, shared chemical scaffolds resembling triclosan. Further investigations involving drug combinations identified five synergistic drug combinations, suggesting potential avenues for therapeutic development. The combination of MUT056399 and brilacidin against A. baumannii QS17-1084 and that of MUT056399 and eravacycline against A. baumannii 5075 showed bactericidal activity. These combinations significantly inhibited biofilm formation produced by both A. baumannii strains. Our findings highlight the drug combinations as promising candidates for further evaluation in murine wound infection models against multidrug-resistant A. baumannii. These compounds hold potential for addressing the critical need for effective antibiotics in the face of rising antimicrobial resistance.
Rice yield prediction through integration of biophysical parameters with SAR and optical remote sensing data using machine learning models
Authors: Sah Sonam , Haldar Dipanwita , Singh RN , Das B. , Nain Ajeet Singh
Published: September 2024

In an era marked by growing global population and climate variability, ensuring food security has become a paramount concern. Rice, being a staple crop for billions of people, requires accurate and timely yield prediction to ensure global food security. This study was undertaken across two rice crop seasons in the Udham Singh Nagar district of Uttarakhand state to predict rice yield at 45, 60 and 90 days after transplanting (DAT) through machine learning (ML) models, utilizing a combination of optical and Synthetic Aperture Radar (SAR) data in conjunction with crop biophysical parameters. Results revealed that the ML models were able to provide relatively accurate early yield estimates. For summer rice, eXtreme gradient boosting (XGB) was the best-performing model at all three stages (45, 60, and 90 DAT), while for kharif rice, the best-performing models at 45, 60, and 90 DAT were XGB, Neural network (NNET), and Cubist, respectively. The combined ranking of ML models showed that prediction accuracy improved as the prediction date approaches harvest, and the best prediction of yield was observed at 90 DAT for both summer and kharif rice. Overall rankings indicate that for summer rice, the top three models were XGB, NNET, and Support vector regression, while for kharif rice, these were Cubist, NNET, and Random Forest, respectively. The findings of this study offer valuable insights into the potential of the combined use of remote sensing and biophysical parameters using ML models, which enhances food security planning and resource management by enabling more informed decision-making by stakeholders such as farmers, policy planners as well as researchers.
An intranasal cationic liposomal polysaccharide vaccine elicits humoral immune responses against Streptococcus pneumoniae
Authors: Wei Peng , Romanò Cecilia , Li Chengxin , Clergeaud Gael , Andresen Thomas L. , Henriksen Jonas R. , Hansen Anders E. , Clausen Mads H.
Published: September 2024

Diseases caused by S. pneumoniae are the leading cause of child mortality. As antibiotic resistance of S. pneumoniae is rising, vaccination remains the most recommended solution. However, the existing pneumococcal polysaccharides vaccine (Pneumovax® 23) proved only to induce T-independent immunity, and strict cold chain dependence of the protein conjugate vaccine impedes its promotion in developing countries, where infections are most problematic. Affordable and efficient vaccines against pneumococcus are therefore in high demand. Here, we present an intranasal vaccine Lipo+CPS12F&αGC, containing the capsular polysaccharides of S. pneumoniae 12F and the iNKT agonist α-galactosylceramide in cationic liposomes. In BALB/cJRj mice, the vaccine effectively activates iNKT cells and promotes B cells maturation, stimulates affinity-matured IgA and IgG production in both the respiratory tract and systemic blood, and displays sufficient protection both in vivo and in vitro. The designed vaccine is a promising, cost-effective solution against pneumococcus, which can be expanded to cover more serotypes and pathogens.
Direct visualization of viscous dissipation and wetting ridge geometry on lubricant-infused surfaces
Authors: Naga Abhinav , Rennick Michael , Hauer Lukas , Wong William S. Y. , Sharifi-Aghili Azadeh , Vollmer Doris , Kusumaatmaja Halim
Published: September 2024

Drops are exceptionally mobile on lubricant-infused surfaces, yet they exhibit fundamentally different dynamics than on traditional superhydrophobic surfaces due to the formation of a wetting ridge around the drop. Despite the importance of the wetting ridge in controlling drop motion, it is unclear how it dissipates energy and changes shape during motion. Here, we use lattice Boltzmann simulations and confocal microscopy to image how the wetting ridge evolves with speed, and construct heatmaps to visualize where energy is dissipated on flat and rough lubricated surfaces. As speed increases, the wetting ridge height decreases according to a power law, and an asymmetry develops between the front and rear sides. Most of the dissipation in the lubricant ( >75%) occurs directly in front and behind the drop. The geometry of the underlying solid surface hardly affects the dissipation mechanism, implying that future designs should focus on optimizing the surface geometry to maximize lubricant retention.
Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis
Authors: Chaher Nadia , Lacerda Sara , Digilio Giuseppe , Padovan Sergio , Gao Ling , Lavin Begoña , Stefania Rachele , Velasco Carlos , Cruz Gastão , Prieto Claudia , Botnar René M. , Phinikaridou Alkystis
Published: September 2024

Heart failure (HF) affects 64 million people globally with enormous societal and healthcare costs. Myocardial fibrosis, characterised by changes in collagen content drives HF. Despite evidence that collagen type III (COL3) content changes during myocardial fibrosis, in vivo imaging of COL3 has not been achieved. Here, we discovered the first imaging probe that binds to COL3 with high affinity and specificity, by screening candidate peptide-based probes. Characterisation of the probe showed favourable magnetic and biodistribution properties. The probe’s potential for in vivo molecular cardiac magnetic resonance imaging was evaluated in a murine model of myocardial infarction. Using the new probe, we were able to map and quantify, previously undetectable, spatiotemporal changes in COL3 after myocardial infarction and monitor response to treatment. This innovative probe provides a promising tool to non-invasively study the unexplored roles of COL3 in cardiac fibrosis and other cardiovascular conditions marked by changes in COL3.