bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychophysiological insights and user perspectives: enhancing police de-escalation skills through full-body VR training
Authors: Muñoz John E. , Lavoie Jennifer A. , Pope Alan T.
Published: September 2024

In recent years, Virtual Reality (VR) has emerged as a promising tool for enhancing training responses in high-stress professions, notably among police officers. This study investigates the psychophysiological responses and subjective user experience of active police officers undergoing Mental Health Crisis Response (MHCR) training using an immersive full-body VR system. A total of 10 active police officers with Special Weapons and Tactics (SWAT) training participated in our controlled study. Officers independently took part in one VR training session lasting 7–12 min involving an avatar in crisis portrayed by an actor. Officers wore integrated cardiovascular and electrodermal activity measurement devices for physiological monitoring. VR user experience aspects such as induced symptoms or game mechanics were investigated upon completing the training, aiming to evaluate the officer’s perceptions of the technology. We used the DePICT™ scale to evaluate the de-escalation skills of officers, coded by a research professional. Our findings revealed significant differences in heart rate and heart rate variability responses between baseline and VR scenario immersion, suggesting heightened stress regulation during the MHCR simulation using full-body VR. Arousal measurements also revealed measurable responses during the training in VR. Additionally, the user experience assessment indicated a positive reception to the VR training, with minimal VR-induced symptoms. A “Defensive-Dynamics-Dichotomy” was revealed highlighting dominant autonomic responses linked to defensive actions (e.g., officers who drew a weapon; those who kept their weapons holstered) and their respective implications for stress management and cognitive function. A unique constellation of de-escalation skills was revealed among officers who relied on weapons relative to those who did not, to resolve the scenario. The study highlighted the perceived utility of physiological monitoring technologies in enhancing police training outcomes. In conclusion, our research underscores the potential of VR as an effective tool for de-escalation training following MHCR simulated scenarios among active police officers, offering insights into its psychophysiological impact and user experience. The findings contribute to improving our understanding of the physiology associated with decision-making in police officers to draw a weapon, emphasizing the role of advanced simulation and physiological monitoring technology in developing evidence-based training programs for public safety.
“We call it Bokoa jwa tlhaloganyo”: Setswana parents’ perspective on autism spectrum disorder
Authors: Melamu Neo Jeanett , Tsabedze Wandile Fundo , Erasmus Petro , Schlebusch Liezl
Published: September 2024

Introduction

There is a dearth of knowledge in South Africa about the incidence, prevalence, and effect of autism spectrum disorder (ASD). Consequently, national autism data is outdated, and World Health Organization (WHO) prevalence rates are being used.

Methods

This study focused on Ngaka Modiri Molema District to explore the cultural perspective of ASD in the Setswana culture from a parental or caregiver perspective, specifically those who attended the World Health Organization Caregiver Skills Training (WHO-CST) on ASD. This qualitative study used a phenomenological design and purposively sampled 6 out of 12 participants who wererecipients of WHO-CST. Semi-structured interviews, audio recordings, and field notes were used to collect data.

Results

The study found five main themes: understanding autism, indigenous perceptions of ASD, ways of interacting with children living with autism spectrum disorder, creating a friendly environment and symptoms of ASD.

Discussion

It was concluded that there is a lack of knowledge in Setswana culture about what ASD entails, and there are still some superstitious beliefs regarding ASD, resulting in late diagnoses. ASD studies with larger sample sizes, including medical professionals and policymakers, are recommended.
Symptom vs context: lessons learned from a large-scale implementation of the Cultural Formulation Interview
Authors: Silvius Linda , Antezana J. Katrina V. , Ghane Samrad
Published: September 2024

Mental health services in multicultural societies require culturally sensitive approaches to reduce health disparities. The Cultural Formulation Interview (CFI) is thought to enhance shared decision making and to facilitate culturally and contextually informed treatment. There is, however, little known regarding its implementability in large-scale psychiatric services. The current paper reports on (a) efforts to implement the CFI in a large organization for mental health services in the Netherlands, and (b) two studies that evaluated this implementation process and identified barriers to CFI adoption in clinical practice. Implementation of the CFI was facilitated by developing an online course, an advanced training of “team coaches”, (digital) resources, and integrating the CFI questions into the intake format. A preliminary evaluation revealed that the CFI was administered in only 13.2% of all intakes across the entire organization, with minimal utilization of training resources. Study 1 aimed to investigate how the CFI was perceived by clinicians and stakeholders. A survey of 150 clinicians found a great lack of familiarity with the CFI and its purpose. While 67% reported partial CFI use, 50% saw no added value, and 61% deemed it relevant only for ethnic minorities. Study 2 examined which patient and clinician variables were associated with adequate CFI use (i.e., correct documentation of the CFI information in initial intake reports). The sample consisted of 112 intakes of patients conducted by ten clinicians. Regression analysis showed a significant association between clinicians’ cultural competences and adequate CFI use, meaning that more culturally competent clinicians tended to generate better cultural assessments using the CFI. In addition, the CFI information was documented more adequately among patients who were unemployed at the time of assessment. In conclusion, implementation of the CFI requires a fundamental rethinking of the entire intake assessment, shifting it from a symptom-oriented approach towards a context- and person-centered one. Future trainings may benefit from embedding the CFI within a broader cultural competency training, rather than solely focusing on the CFI, which is currently the common practice.
Analysis of the effect of BMI on depression and anxiety among older adults in China: the mediating role of ADL and IADL
Authors: Qiao Ziqing , Wang Zichun , Qiu Jiaping , Zhang Jie , Cao Weiyi
Published: September 2024

Background

Anxiety and depression are serious psychological and public health issues among the older adults. Currently, there is a lack of understanding of the relationship between Body Mass Index (BMI) and anxiety or depression symptoms in the older adult population in China. Therefore, the purpose of this study is to investigate the impact of BMI on anxiety and depression through correlation analysis in different subgroups and to examine the potential chain mediating effects of Activities of Daily Living (ADL) and Instrumental Activities of Daily Living (IADL) between BMI and symptoms of anxiety and depression.

Methods

From the CLHLS database conducted in 2017–2018, data regarding height, weight, anxiety symptoms, depression symptoms, as well as demographic, socioeconomic, behavioral, and health-related characteristics were collected. Multivariate logistic regression analysis was used to explore the impact of BMI on anxiety and depression symptoms. Finally, the SPSS macro process was utilized to test the multiple mediating effects of ADL and IADL between BMI and anxiety or depression symptoms.

Results

After screening, a final sample of 9,098 Chinese older adult individuals aged 65 and above was selected. Among them, 1,074 cases (11.8%) exhibited anxiety symptoms, 1,458 cases (16.0%) exhibited depressive symptoms. Compared to normal BMI, the adjusted analysis showed that underweight in Chinese older adult individuals was significantly associated with anxiety (OR = 1.316, p = 0.004) and depression (OR = 1.232, p = 0.013). This relationship was found to be more significant in males, individuals aged 80 and above, unmarried individuals, and those residing in town. ADL and IADL played a chain-mediated role between BMI and anxiety symptoms in the older adult. BMI not only had a direct effect on anxiety symptoms in the older adult (effect = −0.0159; SE = 0.0066; 95%CI: LL = −0.0288, UL = −0.0031), but also influenced them indirectly through two pathways: the independent mediating role of IADL (effect = −0.0010; SE = 0.0005; 95%CI: LL = −0.0018, UL = −0.0003) and the chain-mediated role of ADL and IADL (effect = −0.0012; SE = 0.0004; 95%CI: LL = −0.0020, UL = −0.0006).

Conclusion

In the older adult individuals in China, underweight is associated with an increased risk of anxiety and depression symptoms. Improving the underweight condition of Chinese older adult individuals can reduce their ADL and IADL limitations, thereby benefiting their mental health.
Tracking sero-molecular trends of swine brucellosis in Hawai‘i and the central Pacific
Authors: La Thi Hai Au , McMillan Ian A. , Dahal Prashant , Burger Andrew H. , Belcaid Mahdi , Phelps Darrin M. , Goldstein Samuel M. , Brown Vienna R. , Norris Michael H.
Published: September 2024

Introduction

Brucellosis is a zoonotic disease of mammals caused by bacterial species of the Brucella genus. The reservoir for disease is typically mammals, with species of Brucella found infecting amphibians, bats, and marine mammals. Brucella spp. can pass directly to humans through contact with infected animals or their products. Brucella spp. can cause chronic debilitating infections in mammals, including humans, and is associated with spontaneous abortions in infected animals, causing reduced fecundity. In Hawai‘i, terrestrial species that could harbor Brucella spp. include swine, cattle, horses, and axis deer among others. The numerous feral swine in Hawai‘i are known to carry Brucella suis, with evidence supporting infections in cattle. Brucella suis also poses infection risk to humans, dogs, and potentially horses across the state.

Methods

In this study, 3,274 feral swine serum samples collected from 5 of the 8 main islands over a 15-year span were analyzed for exposure to B. suis. Of the 558 watersheds in the state, 77 were sampled as part of this effort. Spatial analysis was used to identify watersheds of concern. MLVA and whole genome SNP analysis was used for molecular epidemiological analysis.

Results

Statewide seropositivity rates were triple that of feral swine found in the conterminous United States. Smoothed positivity rates were highest on Maui, followed by O‘ahu, and the island of Hawai‘i. Island-by-island analysis found high brucellosis positivity levels associated with specific watersheds and agricultural areas. Local spatial autocorrelation identified hot spots on O‘ahu and Hawai‘i. MLVA analysis of available B. suis from Hawai‘i found molecular epidemiological connections with B. suis found in French Polynesia and the mainland US while differing from those in Tonga, Western Polynesia. Strains from Hawai‘i are phylogenetically closest to strains from the United States. MLVA and SNP analysis found B. suis strains from Hawai‘i fell into the genetic group that contains biovar 1 B. suis.

Discussion

This work identified islands and watersheds of high brucellosis seropositivity in feral swine of Hawai‘i, highlighting the magnitude of the zoonotic risk. Introduction of strains in recent history is unlikely due to modern animal trade and disease control practices. Genomic analysis of strains in Hawai‘i and the Pacific area can provide hidden historical and local clues to brucellosis epidemiology in the state.
The influence of service dog partnerships on perceived and objective sleep quality for military veterans with PTSD
Authors: Bristol Stephanie , Leighton Sarah C. , Schwichtenberg A. J. , Campbell Rebecca L. , Ashbeck Erin L. , Taylor Daniel J. , Bedrick Edward J. , O'Haire Marguerite E.
Published: September 2024

Introduction

Sleep disturbances, fear of sleep, and nightmares are among the most reported symptoms for military service members and veterans (henceforth “veterans”) with posttraumatic stress disorder (PTSD), potentially contributing to treatment resistance and heightened suicidality. Despite available evidence-based interventions, many veterans still report difficulties. The complementary intervention of a psychiatric service dog may contribute to improvements in sleep quality for veterans with PTSD.

Methods

This preregistered, non-randomized clinical trial evaluated the association between service dog partnership and sleep at 3 month follow-up. Participants were N = 155 veterans with PTSD (81 in the service dog group and 74 waitlisted controls). Measures included self-report surveys measuring PTSD severity (PTSD Checklist for DSM-5, PCL-5) sleep quality (Pittsburgh Sleep Quality Index, PSQI), sleep disturbance (PROMIS Sleep Disturbance), and fear of sleep (Fear of Sleep Inventory-Short Form, FoSI-SF); morning sleep diaries measuring nightmares; and wrist-worn actigraphy. Regression models and mediation analyses were performed.

Results

Service dog partnership was significantly associated with better subjective sleep [PSQI: mean difference −2.2, 95% CI (−3.4, −1.1), p < 0.001; PROMIS Sleep Disturbance: −3.6, 95% CI (−6.3, −0.9), p = 0.004; FoSI-SF: −6.6, 95% CI (−9.7, −3.5), p < 0.001] and odds of nightmares [OR = 0.45, 95% CI (0.26, 0.76), p = 0.003]. Service dog partnership was not associated with a change in actigraphy-based measures of sleep [sleep onset: −4.5, 95% CI (−12.2, 3.2); sleep duration: −4.7, 95% CI (−25.9, 16.6); wake after sleep onset: 6.0, 95% CI (−2.1,14.1); sleep efficiency: −0.4, 95% CI (−3.4, 2.5)]. The estimated proportion of the effect of service dogs on PTSD severity mediated by fear of sleep was 0.26 [95% CI (0.10, 0.48)].

Discussion

Compared to the control group, veterans with service dogs for PTSD reported better sleep quality, less sleep disturbance, and less fear of sleep after 3 months. However, service dog partnership was not associated with differences in actigraphy-measured sleep. These findings demonstrate the impact of psychiatric service dog partnerships on sleep quality for veterans with PTSD.
Cesarian section and long-term outcomes for cownose rays (Rhinoptera bonasus)
Authors: Buckner Chris , George Robert H. , Bulman Frank , Durrett Jared , Handsel Tim , Wyffels Jennifer T.
Published: September 2024

Cownose rays (Rhinoptera bonasus) are schooling rays commonly displayed in large groups in public aquariums. They are long-lived, have an annual reproductive cycle, and readily breed in managed care with most pregnancies culminating with the unaided and successful birth of a single neonate. Occasionally, females are observed to have prolonged pregnancies or suffer dystocia during parturition and intervention via a cesarian section (C-section) is required to deliver the neonate. Monthly reproductive monitoring at Ripley’s Aquarium of the Smokies using ultrasound to stage pregnancies allows for the prediction of anticipated due dates and guides the decision to assist with delivery. Recognizing when to assist birth and best practices for performing C-section are important for the reproductive health, sustainability, and longevity of this species in managed care. This report describes a surgical technique for C-section in cownose rays and includes short-term complications and long-term outcomes for females.
A Diet Supplemented with Oil-Free Olive Pulp Flour (MOP-ManniOlivePowder®) Improves the Oxidative Status of Dogs
Authors: Minieri Sara , Altomonte Iolanda , Bellini Virginia , Casini Lucia , Gazzano Angelo
Published: September 2024

Olive oil coproducts and their phenolic extracts have shown beneficial effects when added to the diets of food-producing animals, whereas data on their effects on pets are scarce. The aim of this study was to evaluate the effects of dietary supplementation with olive flour (MOP®) on oxidative blood biomarkers in dogs. Thirty dogs were recruited and divided into two groups. Both groups were fed the same kibble feed twice daily. The treatment group (T) also received canned wet feed supplemented with 11.5 mg/kg of body weight of organic olive flour per day, whereas the control group (C) received the same wet feed without any supplementation. The findings showed that oil-free olive pulp flour supplementation led to a significant decrease in d-ROMs (p < 0.044) in the blood of the T group (from 101.26 to 86.67 U CARR), whereas no significant changes were observed in the C group. An increasing OXY trend was found in the blood of the T group. Polyphenols in olive flour at a dose of 11.5 mg/kg of body weight contributed to lowering the oxidative stress threshold in dogs, reducing the levels of d-ROMs in dogs and leading to increasing trends in the amount of blood antioxidants. The use of olive pulp flour in dog diets has proven to be beneficial for their health and could also reduce the waste associated with olive oil production.
Comparative Morphology of Skeletal Development in Homo sapiens and Raja asterias: Divergent Stiffening Patterns Due to Different Matrix Calcification Processes
Authors: Pazzaglia Ugo E. , Zecca Piero A. , Terova Genciana , Serena Fabrizio , Mancusi Cecilia , Raimondi Giovanni , Zarattini Guido , Raspanti Mario , Reguzzoni Marcella
Published: September 2024

Before calcification begins, the early embryonic and fetal skeletal development of both mammalian Homo sapiens and the chondrichthyan fish Raja asterias consists exclusively of cartilage. This cartilage is formed and shaped through processes involving tissue segmentation and the frequency, distribution, and orientation of chondrocyte mitoses. In the subsequent developmental phase, mineral deposition in the cartilage matrix conditions the development further. The stiffness and structural layout of the mineralized cartilage have a significant impact on the shape of the anlagen (early formative structure of a tissue, a scaffold on which the new bone is formed) and the mechanical properties of the skeletal segments. The fundamental difference between the two studied species lies in how calcified cartilage serves as a scaffold for osteoblasts to deposit bone matrix, which is then remodeled. In contrast, chondrichthyans retain the calcified cartilage as the definitive skeletal structure. This study documents the distinct mineral deposition pattern in the cartilage of the chondrichthyan R. asterias, in which calcification progresses with the formation of focal calcification nuclei or “tesserae”. These are arranged on the flat surface of the endo-skeleton (crustal pattern) or aligned in columns (catenated pattern) in the radials of the appendicular skeleton. This anatomical structure is well adapted to meet the mechanical requirements of locomotion in the water column. Conversely, in terrestrial mammals, endochondral ossification (associated with the remodeling of the calcified matrix) provides limb bones with the necessary stiffness to withstand the strong bending and twisting stresses of terrestrial locomotion. In this study, radiographs of marine mammals (reproduced from previously published studies) document how the endochondral ossification in dolphin flippers adapts to the mechanical demands of aquatic locomotion. This adaptation includes the reduction in the length of the stylopodium and zeugopodium and an increase in the number of elements in the autopodium’s central rays.
Addition of Chicken Litter Compost Changes Bacteriobiome in Fallow Soil
Authors: Naumova Natalia , Barsukov Pavel , Baturina Olga , Rusalimova Olga , Kabilov Marsel
Published: September 2024

Composting is an environmentally friendly process, turning animal waste into fertilizer. Chicken litter compost (CLC) improves soil properties, increasing crop yields. However, the CLC effect on the soil microbiome is understudied. This study aimed to compare bacteriobiome diversity in fallow arable Chernozem with and without CLC addition in a field experiment in the Novosibirsk region, Russia, using 16S rRNA gene metabarcoding. Pseudomonadota, Actinomycetota and Acidobacteriota were the most OTU-rich phyla, together accounting for >50% of the total number of sequence reads. CLC-related shifts in the bacteriobiome structure occurred at all taxonomic levels: the Bacillota abundance was 10-fold increased due to increased Bacilli, both being indicator taxa for the CLC-soil. The main Actinomycetota classes were the indicators for the CLC-soil (Actinobacteria) and no-CLC soil (Thermoleophilia, represented Gaiella). Both Bacillota and Actinomycetota phyla were the ultimate constituents of the CLC added, persisting in the soil for five months of fallowing. The no-CLC soil indicator phyla were Acidobacteriota (represented by Acidobacteria_Group3) and Verrucomicrobiota. Future metabarcoding studies of chicken litter application in agricultural soils, including cropped studies, should address the soil microbiome at the species/strain levels in more detail, as well as how it is affected by specific crops, preferably accompanied by a direct methodology revealing the microbiota functions.
A Patient-Centered Conceptual Model of AYA Cancer Survivorship Care Informed by a Qualitative Interview Study
Authors: Figueroa Gray Marlaine S. , Shapiro Lily , Dorsey Caitlin N. , Randall Sarah , Casperson Mallory , Chawla Neetu , Zebrack Brad , Fujii Monica M. , Hahn Erin E. , Keegan Theresa H. M. , Kirchhoff Anne C. , Kushi Lawrence H. , Nichols Hazel B. , Wernli Karen J. , Sauder Candice A. M. , Chubak Jessica
Published: September 2024

Purpose: Conceptual models provide frameworks to illustrate relationships among patient-, provider-, system-, and community-level factors that inform care delivery and research. Existing models of cancer survivorship care focus largely on pediatric or adult populations whose needs differ from adolescents and young adults (AYAs). We developed a patient-centered conceptual model of AYA survivorship care. Methods: We conducted a narrative literature review of current conceptual and theoretical models of care. We engaged AYA cancer survivors (n = 25) in semi-structured one-hour telephone interviews. Most participants were in their 20s and 30s, and the majority (84%) were women. Recruitment was stratified by age and time since cancer diagnosis. We conducted a thematic analysis of interview transcripts to identify themes that exemplified patient-centered care. Results: Most participants identified as white and female. Leukemia and breast cancer were the most common cancer types. Main themes included the need for (1) care coordination, (2) ongoing mental health support, (3) connection to AYA peer support, (4) support during fertility preservation efforts, (5) support with financial burden, (6) support for quality of life, (7) information about and support with side effects and late effects, and (8) attention to the unique needs of young adults. Conclusions: We present a patient-centered conceptual model of AYA survivorship care needs that can inform future cancer care delivery and research.
Improving the Clinical Utility of Platelet Count for Cancer Detection in Primary Care: A Cohort Study in England, Canada, and Australia
Authors: Mounce Luke T. A. , Calitri Raff , Hamilton Willie , Rafiq Meena , Emery Jon D. , Giannakeas Vasily , Kotsopoulos Joanne , Bailey Sarah E. R.
Published: September 2024

The platelet count, a component of the full blood count, has been identified as a useful diagnostic marker for cancer in primary care. The reference range for the platelet count is 150 to 400 or 450 × 109/L; this range does not account for natural variation in platelet count by age and sex. This study used three primary care cohorts from England, Canada, and Australia. Patients aged 40 years and over with a full blood count were included and stratified by age (in 10-year bands), sex, (male/female), and platelet count group. Cancer incidence within one year of the test date was estimated from linked registry data. In all three countries, there was a clear upwards trend in cancer incidence with increasing platelet count for both sexes and at all age groups. Lung and colorectal were the most common sites. These results have important implications for the international application of this work; analysis of local health datasets will be crucial to determining appropriate thresholds. Appropriate upper thresholds will depend on local populations, healthcare needs, and priorities. Further research is needed to assess the likely impact of new recommendations on the healthcare system, on cancer outcomes, and patient benefit.
Therapeutic Advances in Advanced Basal Cell Carcinoma
Authors: Alkassis Samer , Shatta Maya , Wong Deborah J.
Published: September 2024

Basal cell carcinoma (BCC) is the most common type of cancer with an estimated 3.6 million cases diagnosed annually in the US alone. While most cases are treatable with low recurrence rates, 1–10% progress to an advanced stage which can behave aggressively, leading to local destruction and posing substantial challenges in management. The pathogenesis often involves dysregulation of the patched/hedgehog protein family, a pivotal pathway targeted by recently approved therapies. Furthermore, the role of immunotherapy is evolving in this type of tumor as we learn more about tumor microenvironment dynamics. In recent years, there have been advancements in the therapeutic landscape of advanced BCC, offering patients new hope and options for managing this complex and potentially life-threatening condition. In this review, we aim to provide a comprehensive overview of this disease, including the risk factors, underlying pathogenesis, current treatment options of advanced disease, and the ongoing exploration and development of novel therapies.
The Impact of Melanoma Imaging Biomarker Cues on Detection Sensitivity and Specificity in Melanoma versus Clinically Atypical Nevi
Authors: Agüero Rosario , Buchanan Kendall L. , Navarrete-Dechent Cristián , Marghoob Ashfaq A. , Stein Jennifer A. , Landy Michael S. , Leachman Sancy A. , Linden Kenneth G. , Garcet Sandra , Krueger James G. , Gareau Daniel S.
Published: September 2024

Incorporation of dermoscopy and artificial intelligence (AI) is improving healthcare professionals’ ability to diagnose melanoma earlier, but these algorithms often suffer from a “black box” issue, where decision-making processes are not transparent, limiting their utility for training healthcare providers. To address this, an automated approach for generating melanoma imaging biomarker cues (IBCs), which mimics the screening cues used by expert dermoscopists, was developed. This study created a one-minute learning environment where dermatologists adopted a sensory cue integration algorithm to combine a single IBC with a risk score built on many IBCs, then immediately tested their performance in differentiating melanoma from benign nevi. Ten participants evaluated 78 dermoscopic images, comprised of 39 melanomas and 39 nevi, first without IBCs and then with IBCs. Participants classified each image as melanoma or nevus in both experimental conditions, enabling direct comparative analysis through paired data. With IBCs, average sensitivity improved significantly from 73.69% to 81.57% (p = 0.0051), and the average specificity improved from 60.50% to 67.25% (p = 0.059) for the diagnosis of melanoma. The index of discriminability (d′) increased significantly by 0.47 (p = 0.002). Therefore, the incorporation of IBCs can significantly improve physicians’ sensitivity in melanoma diagnosis. While more research is needed to validate this approach across other healthcare providers, its use may positively impact melanoma screening practices.
Simulating the Effect of Removing Circulating Tumor Cells (CTCs) from Blood Reveals That Only Implantable Devices Can Significantly Reduce Metastatic Burden of Patients
Authors: Baumgartner Werner , Aceto Nicola , Lifka Sebastian
Published: September 2024

Circulating tumor cells (CTCs) are cells that have separated from a solid cancerous lesion and entered the bloodstream. They play a crucial role in driving the metastatic spread to distant organs, which is the leading cause of cancer-related deaths. Various concepts for blood purification devices aiming to remove CTCs from the blood and prevent metastases have been developed. Until now, it is not clear if such devices can indeed reduce new metastasis formation in a significant way. Here, we present a simple theoretical model of CTCs in the bloodstream that can be used to predict a reduction in metastatic burden using an extracorporeal or intracorporeal blood purification device. The model consists of a system of ordinary differential equations that was numerically solved and simulated. Various simulations with different parameter settings of extracorporeal and intracorporeal devices revealed that only devices implanted directly in tumor-draining vessels can reduce the metastatic burden significantly. Even if an extracorporeal device is used permanently, the reduction in metastases is only 82%, while a permanently operating implanted device in the tumor-draining vessel would achieve a reduction of 99.8%. These results are mainly due to the fact that only a small fraction of CTCs reaches peripheral circulation, resulting in a proportionally small amount of purified blood in extracorporeal devices.
Supplementation with Fish Oil and Selenium Protects Lipolytic and Thermogenic Depletion of Adipose in Cachectic Mice Treated with an EGFR Inhibitor
Authors: Wang Hang , Chan Yi-Lin , Chiu Yi-Han , Wu Tsung-Han , Hsia Simon , Wu Chang-Jer
Published: September 2024

Lung cancer and cachexia are the leading causes of cancer-related deaths worldwide. Cachexia is manifested by weight loss and white adipose tissue (WAT) atrophy. Limited nutritional supplements are conducive to lung cancer patients, whereas the underlying mechanisms are poorly understood. In this study, we used a murine cancer cachexia model to investigate the effects of a nutritional formula (NuF) rich in fish oil and selenium yeast as an adjuvant to enhance the drug efficacy of an EGFR inhibitor (Tarceva). In contrast to the healthy control, tumor-bearing mice exhibited severe cachexia symptoms, including tissue wasting, hypoalbuminemia, and a lower food efficiency ratio. Experimentally, Tarceva reduced pEGFR and HIF-1α expression. NuF decreased the expression of pEGFR and HIF-2α, suggesting that Tarceva and NuF act differently in prohibiting tumor growth and subsequent metastasis. NuF blocked LLC tumor-induced PTHrP and expression of thermogenic factor UCP1 and lipolytic enzymes (ATGL and HSL) in WAT. NuF attenuated tumor progression, inhibited PTHrP-induced adipose tissue browning, and maintained adipose tissue integrity by modulating heat shock protein (HSP) 72. Added together, Tarceva in synergy with NuF favorably improves cancer cachexia as well as drug efficacy.
Modulation of Multidrug Resistance Transporters by Food Components and Dietary Supplements: Implications for Cancer Therapy Efficacy and Safety
Authors: Brodzicka Agnieszka , Galanty Agnieszka , Paśko Paweł
Published: September 2024

The aim of this review is to explore how diet and dietary supplements influence the activity of key multidrug resistance (MDR) transporters—MRP2, BCRP, and P-gp. These transporters play a crucial role in drug efflux from cancer cells and significantly affect chemotherapy outcomes. This review focuses on how dietary phytochemicals, such as catechins and quercetin, impact the expression and function of these transporters. Both in vitro and in vivo experiments were examined to assess changes in drug bioavailability and intracellular drug accumulation. The findings show that certain dietary components—such as catechins, flavonoids, resveratrol, curcumin, terpenoids, sterols, and alkaloids—can either inhibit or induce MDR transporter activity, thus influencing the effectiveness of chemotherapy. These results highlight the importance of understanding diet–drug interactions in cancer therapy to improve treatment outcomes and reduce side effects. In conclusion, dietary modifications and supplements should be carefully considered in cancer treatment plans to optimize therapeutic efficacy.
The Diterpene Isopimaric Acid Modulates the Phytohormone Pathway to Promote Oryza sativa L. Rice Seedling Growth
Authors: Huang Jiaqi , Hua Juan , Peng Luying , Bai Liping , Luo Shihong
Published: September 2024

Many plant secondary metabolites are active and important in the regulation of plant growth. Certain plant-derived diterpenes are known to promote plant growth, but the pathways by which this promotion occurs are still unknown. Activity screening revealed that the plant-derived diterpene isopimaric acid exhibits growth-promoting activity in rice (Oryza sativa L.) seedlings. Furthermore, 25 μg/mL of isopimaric acid promoted the growth of 15 self-incompatible associated populations from different rice lineages to different extents. Quantitative analyses revealed a significant decrease in the concentration of the defense-related phytohormone abscisic acid (ABA) following treatment with isopimaric acid. Correlation analysis of the phytohormone concentrations with growth characteristics revealed that the length of seedling shoots was significantly negatively correlated with concentrations of 3-indole-butyric acid (IBA). Moreover, the total root weight was not only negatively correlated with ABA concentrations but also negatively correlated with concentrations of isopentenyl adenine (iP). These data suggest that isopimaric acid is able to influence the phytohormone pathway to balance energy allocation between growth and defense in rice seedlings and also alter the correlation between the concentrations of phytohormones and traits such as shoot and root length and weight. We provide a theoretical basis for the development and utilization of isopimaric acid as a plant growth regulator for rice.
Analysis of Codon Usage Bias in the Plastid Genome of Diplandrorchis sinica (Orchidaceae)
Authors: Chen Xuhui , Zhao Yudi , Xu Shenghua , Zhou Yingze , Zhang Lijie , Qu Bo , Xu Yufeng
Published: September 2024

In order to understand the bias and main affecting factors of codon usage in the plastid genome of Diplandrorchis sinica, which is a rare and endangered plant species in the Orchidaceae family, the complete plastid genome sequence of D. sinica was downloaded from the GenBank database and 20 protein-coding sequences that met the analysis requirements were finally selected. The GC content, length of the amino acid (Laa), relative synonymous codon usage (RSCU), and effective number of codon (ENC) of each gene and codon were calculated using the CodonW and EMBOSS online programs. Neutral plot analysis, ENC-plot analysis, PR2-plot analysis, and correspondence analysis were performed using Origin Pro 2024 software, and correlation analysis between various indicators was performed using SPSS 23.0 software. The results showed that the third base of the codon in the plastid genome of D. sinica was rich in A and T, with a GC3 content of 27%, which was lower than that of GC1 (45%) and GC2 (39%). The ENC value ranged from 35 to 57, with an average of 47. The codon usage bias was relatively low, and there was a significant positive correlation between ENC and GC3. There were a total of 32 codons with RSCU values greater than 1, of which 30 ended with either A or U. There were a total of nine optimal codons identified, namely, UCU, UCC, UCA, GCA, UUG, AUA, CGU, CGA, and GGU. This study indicated that the dominant factor affecting codon usage bias in the plastid genome of D. sinica was natural selection pressure, while the impact of base mutations was limited. The codon usage patterns were not closely related to gene types, and the distribution of photosynthetic system genes and ribosomal protein-coding gene loci was relatively scattered, indicating significant differences in the usage patterns of these gene codons. In addition, the codon usage patterns may not be related to whether the plant is a photosynthetic autotrophic or heterotrophic nutritional type. The results of this study could provide scientific references for the genomic evolution and phylogenetic research of plant species in the family Orchidaceae.
Exploring the Role of the Processing Body in Plant Abiotic Stress Response
Authors: Huang Zhehao , Xu Zhi , Liu Xiuqing , Chen Gangmin , Hu Chensi , Chen Menglu , Liu Yun
Published: September 2024

The processing body (P-Body) is a membrane-less organelle with stress-resistant functions. Under stress conditions, cells preferentially translate mRNA that favors the stress response, resulting in a large number of transcripts unfavorable to the stress response in the cytoplasm. These non-translating mRNAs aggregate with specific proteins to form P-Bodies, where they are either stored or degraded. The protein composition of P-Bodies varies depending on cell type, developmental stage, and external environmental conditions. This review primarily elucidates the protein composition in plants and the assembly of P-Bodies, and focuses on the mechanisms by which various proteins within the P-Bodies of plants regulate mRNA decapping, degradation, translational repression, and storage at the post-transcriptional level in response to ethylene signaling and abiotic stresses such as drought, high salinity, or extreme temperatures. This overview provides insights into the role of the P-Body in plant abiotic stress responses.
Experimental Study on the Suitability of Waste Plastics and Glass as Partial Replacement of Fine Aggregate in Concrete Production
Authors: Legese Alemu Mosisa , Mitiku Degefe , Feyessa Fekadu Fufa , Urgessa Girum , Boru Yada Tesfaye
Published: September 2024

Solid waste management is a major environmental challenge, especially in developing countries, with increasing amounts of waste glass (WG) and waste plastic (WP) not being recycled. In Ethiopia, managing WG and WP requires innovative recycling techniques. This study examines concrete properties with WG and WP as partial replacements for fine aggregate. Tests were conducted on cement setting time, workability, compressive strength, splitting tensile strength, and flexural strength. Concrete of grade C-25, with a target compressive strength of 25 MPa, was prepared by partially replacing fine aggregate with WP and WG. The mechanical properties were evaluated after 7 and 28 days of curing. At a 20% replacement level, workability decreased at water–cement ratios of 0.5 and 0.6 but remained stable at 0.4, leading to the selection of the 0.4 ratio for further testing. A 10% replacement of fine aggregate, using a ratio of 3% WP and 7% WG, was found to be optimal, resulting in an increase in compressive strength by 12.55% and 6.44% at 7 and 28 days, respectively. In contrast, a 20% replacement led to a decrease in compressive strength by 14.35% and 0.73% at 7 and 28 days, respectively. On the 28th day, the splitting tensile strength at the optimal replacement level was 4.3 MPa, reflecting an 8.5% reduction compared to the control mix. However, flexural strength improved significantly by 19.7%, from 12.46 MPa to 15.52 MPa. Overall, the incorporation of WG and WP in concrete enhances flexural strength but slightly reduces splitting tensile strength.
Enhanced External Counterpulsation Improves Dyspnea, Fatigue, and Functional Capacity in Patients with Long COVID
Authors: Fox Jessie , Ali Farhan , Lopez Marielisa , Shah Sachin A. , Schmidt Christian W. , Quesada Odayme , Henry Timothy D. , Verduzco-Gutierrez Monica
Published: September 2024

Approximately 31% of patients previously infected with SARS-CoV-2 are living with symptoms of long COVID in the United States. Long COVID significantly reduces quality of life and increases morbidity and disability; however, treatment options are limited. Enhanced External Counterpulsation (EECP) is an FDA-approved, non-invasive treatment for the management of cardiovascular symptoms with a mechanism of action which stimulates pathways that induce endothelial homeostasis, improving microvascular function, inflammation, and immune regulation, thereby potentially targeting the underlying etiology of long COVID. We recently reported that EECP improved symptoms in 231 patients with long COVID. Previous studies assessing the effects of EECP for long COVID have lacked a control group. As such, this analysis is the first comparing outcomes in patients with long COVID undergoing EECP (n = 33) to a non-treated group (control, n = 33). The patients were matched for baseline characteristics, and all patients completed patient-reported outcome assessments, including PROMIS Fatigue, the Duke Activity Status Index (DASI), and the Rose Dyspnea Scale (RDS), two times within a specified time interval. When comparing the average change from baseline in both groups, the EECP-treated patients’ improvement was significantly greater than the improvement in the control group across all measured endpoints, including PROMIS Fatigue (−15.0 ± 8.9 vs. −2.8 ± 5.9, p < 0.001) and DASI (+17.8 (11.8, 26.8) vs. +1.8 (−3.5, 5.5), p < 0.001), and there was an improvement of ≥1 in the RDS class (75.8% vs. 33.3%, p < 0.001). This study’s limitations include the small sample size and lack of information regarding concurrent treatments or interventions in the non-treated group; however, these preliminary data support EECP as a potential low-risk treatment option for patients with long COVID.
Systemic Therapy for Metastatic Pancreatic Cancer—Current Landscape and Future Directions
Authors: Netto Daniel , Frizziero Melissa , Foy Victoria , McNamara Mairéad G. , Backen Alison , Hubner Richard A.
Published: September 2024

Pancreatic ductal adenocarcinoma (PDAC) is a significant cause of cancer-associated mortality, with a rising global incidence. A paucity of strong predictive risk factors mean screening programmes are difficult to implement. Historically, a lack of identifiable and actionable driver mutations, coupled with a relatively immunosuppressed tumour microenvironment, has led to a reliance on cytotoxic chemotherapy. The NAPOLI-3 trial has reported data supporting consideration of NALIRIFOX as a new first-line standard of care. Kirsten Rat Sarcoma Virus (KRAS) G12D mutations are present in >90% of all PDAC’s; exciting breakthroughs in small molecule inhibitors targeting KRAS G12D may open new modalities of treatment, and therapies targeting multiple KRAS mutations are also in early clinical trials. Although immunotherapy strategies to date have been disappointing, combination with chemotherapy and/or small molecule inhibitors hold promise and warrant further exploration.
Modeling the Present and Future Geographical Distribution Potential of Dipteronia dyeriana, a Critically Endangered Species from China
Authors: Yan Ming-Hui , Liu Bin-Wen , Tiamiyu Bashir B. , Zhang Yin , Ning Wang-Yang , Si Jie-Ying , Dong Nian-Ci , Lv Xin-Lan
Published: September 2024

Climate change will have various impacts on the survival and development of species, and it is important to explore whether plants can adapt to future climate conditions. Dipteronia dyeriana is an endangered species with a narrow distribution in Yunnan, characterized by a small population size. However, studies on its current distribution and the impact of climate change on its future survival and distribution are very limited. In this study, the current and future (2050 and 2090) potential habitats under the SSP1-2.6, SSP3-7.0, and SSP5-8.5 scenarios were predicted using both maximum entropy (MaxEnt) and random forest (RF) models based on the current range points of D. dyeriana, soil, topographical, land cover, and climate data. The results showed that the RF model demonstrated significantly higher AUC, TSS, and Kappa scores than the MaxEnt model, suggesting high accuracy of the RF model. Isothermality (bio_3), minimum temperature of the coldest month (bio_6), and annual precipitation (bio_12) are the main environmental factors affecting the distribution of D. dyeriana. At present, the high suitability area of D. dyeriana is mainly concentrated in the eastern part of Yunnan Province and part of southern Tibet, covering an area of 3.53 × 104 km2. Under future climate change scenarios, the total area suitable for D. dyeriana is expected to increase. Although, the highly suitable area has a tendency to decrease. With regards to land use, more than 77.53% of the suitable land area (29.67 × 104 km2) could be used for D. dyeriana planting under different SSP scenarios. In 2090, the centroid shifts of the two models exhibit a consistent trend. Under the SSP1-2.6 scenario, the centroids transfer to the southeast. However, under the SSP3-7.0 and SSP5-8.5 scenarios, the centroids of high suitability areas migrate toward the northwest. In summary, this study enhances our understanding of the influence of climate change on the geographic range of D. dyeriana and provides essential theoretical backing for efforts in its conservation and cultivation.
Stability of Breathers for a Periodic Klein–Gordon Equation
Authors: Chirilus-Bruckner Martina , Cuevas-Maraver Jesús , Kevrekidis Panayotis G.
Published: September 2024

The existence of breather-type solutions, i.e., solutions that are periodic in time and exponentially localized in space, is a very unusual feature for continuum, nonlinear wave-type equations. Following an earlier work establishing a theorem for the existence of such structures, we bring to bear a combination of analysis-inspired numerical tools that permit the construction of such waveforms to a desired numerical accuracy. In addition, this enables us to explore their numerical stability. Our computations show that for the spatially heterogeneous form of the ϕ4 model considered herein, the breather solutions are generically unstable. Their instability seems to generically favor the motion of the relevant structures. We expect that these results may inspire further studies towards the identification of stable continuous breathers in spatially heterogeneous, continuum nonlinear wave equation models.
Cooperative Jamming-Based Physical-Layer Group Secret and Private Key Generation
Authors: Fu Shiming , Ling Tong , Yang Jun , Li Yong
Published: September 2024

This paper explores physical layer group key generation in wireless relay networks with a star topology. In this setup, the relay node plays the role of either a trusted or untrusted central node, while one legitimate node (Alice) acts as the reference node. The channel between the relay and Alice serves as the reference channel. To enhance security during the channel measurement stage, a cooperative jamming-based scheme is proposed in this paper. This scheme allows the relay to obtain superimposed channel observations from both the reference channel and other relay channels. Then, a public discussion is utilized to enable all nodes to obtain estimates of the reference channel. Subsequently, the legitimate nodes can agree on a secret key (SK) that remains secret from the eavesdropper (Eve), or a private key (PK) that needs to be secret from both the relay and Eve. This paper also derives the lower and upper bounds of the SK/PK capacity. Notably, it demonstrates that there exists only a small constant difference between the SK/PK upper and lower bounds in the high signal-to-noise ratio (SNR) regime. Simulation results confirm the effectiveness of the proposed scheme for ensuring security and efficiency of group key generation.