bluuebunny/crossref_metadata_2025 huggingface dataset:
A plesiosaurian autopodial element (Plesiosauria indet.) with remarkable articular subchondral surfaces from the Maastrichtian type area (south-east Netherlands)
Authors: Barten Lars P.J. , Mulder Eric W.A. , Jagt John W.M.
Published: April 2024

The first isolated and well-preserved phalanx (autopodial element) of a generically and specifically indeterminate (probably xenopsarian) plesiosaur from the Maastrichtian type area (south-east Netherlands) is described and illustrated. Morphological features of this bone, such as the articular subchondral surfaces, allow it to be distinguished from phalanges of co-occurring mosasaurs (Mosasauridae, Squamata) and could imply rapid growth in plesiosaurs, similar to that observed in extant leatherback turtles. The large size of the phalanx indicates that it originates either from a large juvenile or from a paedomorphic individual. The paucity of plesiosaur remains in biocalcarenites of late Maastrichtian age in the Maastrichtian type area could be explained by the shallow settings, in which these strata were deposited, which may not have offered suitable ecological resources conducive to abundant plesiosaur inhabitation. Presumably, the lack of steep continental slopes in the type Maastrichtian, at which oceanic upwelling of cold, nutrient-rich water supported a higher prey density for pelagic predators such as plesiosaurs, may have inhibited plesiosaur occupation. Rather, decomposing floating carcasses may have been the source of dissociated elements of plesiosaurs in this area, such as isolated teeth, vertebrae and the autopodial element described here.
Are We All Alright? The Influence of Socioeconomic Status on Black Women’s Political Beliefs and Policy Preferences by Region
Authors: Stewart Jessica Lynn , Scott Jamil S.
Published: April 2024

Black women have come to be seen as a dominant force in American politics—particularly in support of the Democratic party. However, this dominance in the political sphere has not translated to dominance in the economic sphere. Despite Black women’s outperformance of their Black male peers in higher education outcomes and overrepresentation in the labor force, there is still an economic gap between Black women and their male counterparts. In addition, regional differences in cost of living have led to diverging local conditions for Black women as well. What do Black women’s socioeconomic outcomes mean for their political ideology and political preferences? Few studies capture intra-group variation among Black women and how the context in which they live may shape their economic and sociopolitical outlook. Using the 2016 Collaborative Multiracial Post-Election Survey, we examine how the relationship between Black women’s socioeconomic status and their political beliefs and the relationship between Black women’s socioeconomic status and political preferences are conditioned by region. We capture the individual factors and regional context that shape differences among Black women in their political beliefs and policy attitudes. This research furthers our understanding of differences in Black women’s politics.
Evaluation of the introduction of a single-lead ECG device and digital cardiologist consultation platform among general practitioners in the Netherlands
Authors: Karregat Evert P.M. , de Koning Marlou A. , Himmelreich Jelle C.L. , Koetsier David W. , de Jong Jonas S.S.G. , Moll van Charante Eric P. , Harskamp Ralf E. , Lucassen Wim A.M.
Published: April 2024

Aim:

To evaluate the use of a single-lead electrocardiography (1L-ECG) device and digital cardiologist consultation platform in diagnosing arrhythmias among general practitioners (GPs).

Background:

Handheld 1L-ECG offers a user-friendly alternative to conventional 12-lead ECG in primary care. While GPs can safely rule out arrhythmias on 1L-ECG recordings, expert consultation is required to confirm suspected arrhythmias. Little is known about GPs’ experiences with both a 1L-ECG device and digital consultation platform for daily practice.

Methods:

We used two distinct methods in this study. First, in an observational study, we collected and described all cases shared by GPs within a digital cardiologist consultation platform initiated by a local GP cooperative. This GP cooperative distributed KardiaMobile 1L-ECG devices among all affiliated GPs (n = 203) and invited them to this consultation platform. In the second part, we used an online questionnaire to evaluate the experiences of these GPs using the KardiaMobile and consultation platform.

Findings:

In total, 98 (48%) GPs participated in this project, of whom 48 (49%) shared 156 cases. The expert panel was able to provide a definitive rhythm interpretation in 130 (83.3%) shared cases and answered in a median of 4 min (IQR: 2–18). GPs responding to the questionnaire (n = 43; 44%) thought the KardiaMobile was of added value for rhythm diagnostics in primary care (n = 42; 98%) and easy to use (n = 41; 95%). Most GPs (n = 36; 84%) valued the feedback from the cardiologists in the consultation platform. GPs experienced this project to have a positive impact on both the quality of care and diagnostic efficiency for patients with (suspected) cardiac arrhythmias. Although we lack a comprehensive picture of experienced impediments by GPs, solving technical issues was mentioned to be helpful for further implementation. More research is needed to explore reasons of GPs not motivated using these tools and to assess real-life clinical impact.
Statistics of Traveling Ionospheric Disturbances at High Latitudes Using a Rapid‐Run Ionosonde
Authors: Moges Samson T. , Sherstyukov Ruslan O. , Kozlovsky Alexander , Ulich Thomas , Lester Mark
Published: April 2024

The potential of deep learning for the investigation of medium scale traveling ionospheric disturbances (MSTIDs) has been exploited through the Sodankylä rapid‐run ionosonde in this statistical study. The complementing observations of the Sodankylä ionosonde with those of the Sodankylä meteor radar reveals the diurnal and seasonal occurrence rate of high‐latitude MSTIDs in the recent low solar activity period, 2018–2020. In our results, the daytime, nighttime and dusk MSTIDs are predominantly identified during winter, summer, and equinoctial months, respectively. The winter daytime higher (lower) occurrence rate is well correlated with the lower (higher) altitude of the height of the F2‐layer peak (hmF2), and the low occurrence rate of the summer daytime is well correlated with the mesosphere‐lower‐thermosphere wind shear and higher gradient of temperature. Relatively high occurrence rate (>0.4) of summer nighttime MSTIDs has a general—but not one‐to‐one agreement—with post‐noon to evening IU (eastward auroral current index) inferred ionospheric conductivity. Rather, we see a one‐to‐one relationship between the summer nighttime MSTIDs and zonal wind shear suggesting that the wind shear‐induced electrodynamic processes could play significant roles for higher occurrence rate of MSTIDs. Furthermore, significant MSTIDs with ∼0.4 occurrence rate are so far revealed during spring and autumn transition periods. The enhanced nighttime MSTID amplitudes during the equinox are observed to be well correlated with IL index (westward auroral current indicator) suggesting that the particle precipitation during substorms could be the primary cause.
Role of Anomalous Ocean Warming on the Intensification of Pre‐Monsoon Tropical Cyclones Over the Northern Bay of Bengal
Authors: Ray Arkaprava , Das Sudeep , Sil Sourav
Published: April 2024

The northern Indian Ocean, typically the Bay of Bengal (BoB), has experienced several stronger tropical cyclones during the pre‐monsoon season in the last few years. Similarly, in 2021, cyclone Yaas reached the Very Severe Cyclonic Storm (VSCS) stage despite forming above 14°N. In comparison to the other three cyclones that formed in 2007 (Akash), 2009 (Aila), and 2017 (Mora) with a similar cyclogenesis location and track, the pre‐cyclonic condition in 2021 is more favorable. It was found that from 2007 to 2021, the area associated with Sea Surface Temperature greater than 31°C increased from 0.1 percent to 29 percent. The Research Moored Array for African‐Asian‐Australian Monsoon Analysis and Prediction buoy at 15°N also recorded an increasing trend of 0.1°C per year in both average and maximum temperatures during May. The warming in 2021 is primarily linked with Marine Heat Wave events, which extensively cover the north BoB for a longer duration starting from the first week of May. Such warming extends even to the subsurface, providing the heat required to convert the depression into VSCS within 2 days. The weaker western boundary current during 2021 made the northern BoB fresher and favorable for cyclone, intensification. The associated anticyclonic eddy along the track extended the abnormal distribution of low saline water even up to the subsurface (∼100m), which is vital in increasing the ocean heat content. These simultaneous pre‐cyclonic oceanic conditions can be incorporated into the weather model to understand and predict the rapid intensification within a shorter duration.
Field Observations Reveal How Plunging Mixing and Sediment Resuspension Affect the Pathway of a Dense River Inflow Into a Deep Stratified Lake
Authors: Blanckaert Koen , Vinnå Love Råman , Bouffard Damien , Lemmin Ulrich , Barry David Andrew
Published: April 2024

The pathway of dense river inflows into lakes, which affects the lake water quality, is not accurately predicted by existing models. The pathway of a dense riverine inflow in a lake with a submerged canyon is analyzed based on measurements during a 4‐month period of weakening lake stratification and weakening density excess between river and epilimnion. In line with models, the dense riverine inflow plunges upon entering the lake, continues as an underflow on the sloping lake bottom, and finally intrudes at its level of neutral buoyancy. Underflow and interflow velocities are O(0.1 m s−1). The river inflow is finally trapped in the pycnocline most of the time, even when the river's density excess and the lake's stratification are marginal. This trapping in the pycnocline is explained by the reduction of the inflow density excess due to the intense plunging mixing, which is an order of magnitude larger than that obtained in confined laboratory flumes. The pathway of the dense riverine inflow is affected by interactions of the underflow with the lake bottom and sedimentary processes. A canyon carved by the underflows confines and accelerates the underflow, which enhances its capacity to entrain and carry sediment. The entrainment of sediment that was previously deposited on the canyon bottom accelerates the underflow. Due to both effects, the underflow can temporarily break through the pycnocline and reach the hypolimnion. Existing models explain these observations qualitatively, but a quantitative prediction would require better parameterizations of the plunging mixing and the sedimentary processes.
How Are Deep Soils Responding to Warming?
Authors: Protti S�nchez Fabrizzio , Malhotra Avni , Schmidt Michael , Rumpel Cornelia , Torn Margaret
Published: April 2024

Scientists aim to integrate observations from deep-soil-warming experiments worldwide to better understand how ecosystems vital to food security and environmental health will react to climate change.
Assessment of Hg Speciation Changes in the Sedimentary Rock Record From Thermal Desorption Characteristics
Authors: Frieling J. , Fendley I. M. , Nawaz M. A. , Mather T. A.
Published: April 2024

Sedimentary mercury (Hg) has become a widely used proxy for paleo‐volcanic activity. However, scavenging and drawdown of Hg by organic‐matter (OM) and sulfides are important non‐volcanic factors determining variability in such records. Most studies, therefore, normalize total Hg (HgT) to a Hg “host‐phase” proxy (e.g., HgT/TOC for OM, HgT/TS for sulfides), with the dominant host‐phase determined based on the strongest observed (linear) correlations. This approach suffers from various non‐linearities in Hg‐host‐phase behavior and does not account for succession‐level, let alone sample‐level, Hg speciation changes. Thermal desorption characteristics or “profiles” (TDPs) for many Hg species during pyrolysis analysis are well‐established with applications including distinguishing between OM‐bound Hg and different Hg sulfides and oxides in (sub‐)recent sediments. We explore the use of TDPs for geological sediment (rock) samples and illustrate the presence of multiple release phases (Hg species)—correlated to geochemical host‐phase—in (almost) all the 65 analyzed Tithonian (146–145 Ma) silt and mudrock samples. By quantifying the Hg in each release phase for every sample, we find TOC concentration may determine ∼60% of the variability in the first (lower temperature) Hg TDP release phase: a stark difference with the total Hg released from these samples, where ∼20% of variation is explained by TOC variability. TDPs provide insight on sample‐level Hg speciation and demonstrate that, while the common assumption of single‐phase Hg speciation in sedimentary rocks is problematic, differences in Hg speciation can be detected, quantified, and accounted for using commonly applied techniques—opening potential for routine assessment.
Dynamics of clonal hematopoiesis under DNA-damaging treatment in patients with ovarian cancer
Authors: Arends Christopher Maximilian , Kopp Klara , Hablesreiter Raphael , Estrada Natalia , Christen Friederike , Moll Ute Martha , Zeillinger Robert , Schmitt Wolfgang Daniel , Sehouli Jalid , Kulbe Hagen , Fleischmann Maximilian , Ray-Coquard Isabelle , Zeimet Alain , Raspagliesi Francesco , Zamagni Claudio , Vergote Ignace , Lorusso Domenica , Concin Nicole , Bullinger Lars , Braicu Elena Ioana , Damm Frederik
Published: April 2024

Clonal hematopoiesis (CH) driven by mutations in the DNA damage response (DDR) pathway is frequent in patients with cancer and is associated with a higher risk of therapy-related myeloid neoplasms (t-MNs). Here, we analyzed 423 serial whole blood and plasma samples from 103 patients with relapsed high-grade ovarian cancer receiving carboplatin, poly(ADP-ribose) polymerase inhibitor (PARPi) and heat shock protein 90 inhibitor (HSP90i) treatment within the phase II EUDARIO trial using error-corrected sequencing of 72 genes. DDR-driven CH was detected in 35% of patients and was associated with longer duration of prior PARPi treatment. TP53- and PPM1D-mutated clones exhibited substantially higher clonal expansion rates than DNMT3A- or TET2-mutated clones during treatment. Expansion of DDR clones correlated with HSP90i exposure across the three study arms and was partially abrogated by the presence of germline mutations related to homologous recombination deficiency. Single-cell DNA sequencing of selected samples revealed clonal exclusivity of DDR mutations, and identified DDR-mutated clones as the origin of t-MN in two investigated cases. Together, these results provide unique insights into the architecture and the preferential selection of DDR-mutated hematopoietic clones under intense DNA-damaging treatment. Specifically, PARPi and HSP90i therapies pose an independent risk for the expansion of DDR-CH in a dose-dependent manner.
Induction of NK cell reactivity against acute myeloid leukemia by Fc-optimized CD276 (B7-H3) antibody
Authors: Stefańczyk Sylwia A. , Hagelstein Ilona , Lutz Martina S. , Müller Stefanie , Holzmayer Samuel J. , Jarjour Grace , Zekri Latifa , Heitmann Jonas S. , Salih Helmut R. , Märklin Melanie
Published: April 2024

Acute myeloid leukemia (AML) remains a therapeutic challenge despite recent therapeutic advances. Although monoclonal antibodies (mAbs) engaging natural killer (NK) cells via antibody-dependent cellular cytotoxicity (ADCC) hold promise in cancer therapy, almost none have received clinical approval for AML, so far. Recently, CD276 (B7-H3) has emerged as a promising target for AML immunotherapy, due to its high expression on leukemic blasts of AML patients. Here, we present the preclinical development of the Fc-optimized CD276 mAb 8H8_SDIE with enhanced CD16 affinity. We demonstrate that 8H8_SDIE specifically binds to CD276 on AML cell lines and primary AML cells and induces pronounced NK cell activation and degranulation as measured by CD69, CD25, and CD107a. Secretion of IFNγ, TNF, granzyme B, granulysin, and perforin, which mediate NK cell effector functions, was induced by 8H8_SDIE. A pronounced target cell-restricted lysis of AML cell lines and primary AML cells was observed in cytotoxicity assays using 8H8_SDIE. Finally, xenograft models with 8H8_SDIE did not cause off-target immune activation and effectively inhibited leukemia growth in vivo. We here present a novel attractive immunotherapeutic compound that potently induces anti-leukemic NK cell reactivity in vitro and in vivo as treatment option for AML.
Role of RB1 in neurodegenerative diseases: inhibition of post-mitotic neuronal apoptosis via Kmt5b
Authors: Zhao Shuang , Mo Guiling , Wang Qiang , Xu Jin , Yu Shihui , Huang Zhibin , Liu Wei , Zhang Wenqing
Published: April 2024

During the development of the vertebrate nervous system, 50% of the nerve cells undergo apoptosis shortly after formation. This process is important for sculpting tissue during morphogenesis and removing transiently functional cells that are no longer needed, ensuring the appropriate number of neurons in each region. Dysregulation of neuronal apoptosis can lead to neurodegenerative diseases. However, the molecular events involved in activating and regulating the neuronal apoptosis program are not fully understood. In this study, we identified several RB1 mutations in patients with neurodegenerative diseases. Then, we used a zebrafish model to investigate the role of Rb1 in neuronal apoptosis. We showed that Rb1-deficient mutants exhibit a significant hindbrain neuronal apoptosis, resulting in increased microglia infiltration. We further revealed that the apoptotic neurons in Rb1-deficient zebrafish were post-mitotic neurons, and Rb1 inhibits the apoptosis of these neurons by regulating bcl2/caspase through binding to Kmt5b. Moreover, using this zebrafish mutant, we verified the pathogenicity of the R621S and L819V mutations of human RB1 in neuronal apoptosis. Collectively, our data indicate that the Rb1-Kmt5b-caspase/bcl2 axis is crucial for protecting post-mitotic neurons from apoptosis and provides an explanation for the pathogenesis of clinically relevant mutations.
Materials design for hypersonics
Authors: Peters Adam B. , Zhang Dajie , Chen Samuel , Ott Catherine , Oses Corey , Curtarolo Stefano , McCue Ian , Pollock Tresa M. , Eswarappa Prameela Suhas
Published: April 2024

Hypersonic vehicles must withstand extreme conditions during flights that exceed five times the speed of sound. These systems have the potential to facilitate rapid access to space, bolster defense capabilities, and create a new paradigm for transcontinental earth-to-earth travel. However, extreme aerothermal environments create significant challenges for vehicle materials and structures. This work addresses the critical need to develop resilient refractory alloys, composites, and ceramics. We will highlight key design principles for critical vehicle areas such as primary structures, thermal protection, and propulsion systems; the role of theory and computation; and strategies for advancing laboratory-scale materials to manufacturable flight-ready components.
Massively parallel screen uncovers many rare 3′ UTR variants regulating mRNA abundance of cancer driver genes
Authors: Fu Ting , Amoah Kofi , Chan Tracey W. , Bahn Jae Hoon , Lee Jae-Hyung , Terrazas Sari , Chong Rockie , Kosuri Sriram , Xiao Xinshu
Published: April 2024

Understanding the function of rare non-coding variants represents a significant challenge. Using MapUTR, a screening method, we studied the function of rare 3′ UTR variants affecting mRNA abundance post-transcriptionally. Among 17,301 rare gnomAD variants, an average of 24.5% were functional, with 70% in cancer-related genes, many in critical cancer pathways. This observation motivated an interrogation of 11,929 somatic mutations, uncovering 3928 (33%) functional mutations in 155 cancer driver genes. Functional MapUTR variants were enriched in microRNA- or protein-binding sites and may underlie outlier gene expression in tumors. Further, we introduce untranslated tumor mutational burden (uTMB), a metric reflecting the amount of somatic functional MapUTR variants of a tumor and show its potential in predicting patient survival. Through prime editing, we characterized three variants in cancer-relevant genes (MFN2, FOSL2, and IRAK1), demonstrating their cancer-driving potential. Our study elucidates the function of tens of thousands of non-coding variants, nominates non-coding cancer driver mutations, and demonstrates their potential contributions to cancer.
Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus
Authors: Herdman Matthew , Isbilir Buse , von Kügelgen Andriko , Schulze Ulrike , Wainman Alan , Bharat Tanmay A. M.
Published: April 2024

Surface layers (S-layers) are proteinaceous, two-dimensional paracrystalline arrays that constitute a major component of the cell envelope in many prokaryotic species. In this study, we investigated S-layer biogenesis in the bacterial model organism Caulobacter crescentus. Fluorescence microscopy revealed localised incorporation of new S-layer at the poles and mid-cell, consistent with regions of cell growth in the cell cycle. Light microscopy and electron cryotomography investigations of drug-treated bacteria revealed that localised S-layer insertion is retained when cell division is inhibited, but is disrupted upon dysregulation of MreB or lipopolysaccharide. We further uncovered that S-layer biogenesis follows new peptidoglycan synthesis and localises to regions of high cell wall turnover. Finally, correlated cryo-light microscopy and electron cryotomographic analysis of regions of S-layer insertion showed the presence of discontinuities in the hexagonal S-layer lattice, contrasting with other S-layers completed by defined symmetric defects. Our findings present insights into how C. crescentus cells form an ordered S-layer on their surface in coordination with the biogenesis of other cell envelope components.
Long-baseline quantum sensor network as dark matter haloscope
Authors: Jiang Min , Hong Taizhou , Hu Dongdong , Chen Yifan , Yang Fengwei , Hu Tao , Yang Xiaodong , Shu Jing , Zhao Yue , Peng Xinhua , Du Jiangfeng
Published: April 2024

Ultralight dark photons constitute a well-motivated candidate for dark matter. A coherent electromagnetic wave is expected to be induced by dark photons when coupled with Standard-Model photons through kinetic mixing mechanism, and should be spatially correlated within the de Broglie wavelength of dark photons. Here we report the first search for correlated dark-photon signals using a long-baseline network of 15 atomic magnetometers, which are situated in two separated meter-scale shield rooms with a distance of about 1700 km. Both the network’s multiple sensors and the shields large size significantly enhance the expected dark-photon electromagnetic signals, and long-baseline measurements confidently reduce many local noise sources. Using this network, we constrain the kinetic mixing coefficient of dark photon dark matter over the mass range 4.1 feV-2.1 peV, which represents the most stringent constraints derived from any terrestrial experiments operating over the aforementioned mass range. Our prospect indicates that future data releases may go beyond the astrophysical constraints from the cosmic microwave background and the plasma heating.
Cdk8/CDK19 promotes mitochondrial fission through Drp1 phosphorylation and can phenotypically suppress pink1 deficiency in Drosophila
Authors: Liao Jenny Zhe , Chung Hyung-lok , Shih Claire , Wong Kenneth Kin Lam , Dutta Debdeep , Nil Zelha , Burns Catherine Grace , Kanca Oguz , Park Ye-Jin , Zuo Zhongyuan , Marcogliese Paul C. , Sew Katherine , Bellen Hugo J. , Verheyen Esther M.
Published: April 2024

Cdk8 in Drosophila is the orthologue of vertebrate CDK8 and CDK19. These proteins have been shown to modulate transcriptional control by RNA polymerase II. We found that neuronal loss of Cdk8 severely reduces fly lifespan and causes bang sensitivity. Remarkably, these defects can be rescued by expression of human CDK19, found in the cytoplasm of neurons, suggesting a non-nuclear function of CDK19/Cdk8. Here we show that Cdk8 plays a critical role in the cytoplasm, with its loss causing elongated mitochondria in both muscles and neurons. We find that endogenous GFP-tagged Cdk8 can be found in both the cytoplasm and nucleus. We show that Cdk8 promotes the phosphorylation of Drp1 at S616, a protein required for mitochondrial fission. Interestingly, Pink1, a mitochondrial kinase implicated in Parkinson’s disease, also phosphorylates Drp1 at the same residue. Indeed, overexpression of Cdk8 significantly suppresses the phenotypes observed in flies with low levels of Pink1, including elevated levels of ROS, mitochondrial dysmorphology, and behavioral defects. In summary, we propose that Pink1 and Cdk8 perform similar functions to promote Drp1-mediated fission.
A CMOS-compatible oscillation-based VO2 Ising machine solver
Authors: Maher Olivier , Jiménez Manuel , Delacour Corentin , Harnack Nele , Núñez Juan , Avedillo María J. , Linares-Barranco Bernabé , Todri-Sanial Aida , Indiveri Giacomo , Karg Siegfried
Published: April 2024

Phase-encoded oscillating neural networks offer compelling advantages over metal-oxide-semiconductor-based technology for tackling complex optimization problems, with promising potential for ultralow power consumption and exceptionally rapid computational performance. In this work, we investigate the ability of these networks to solve optimization problems belonging to the nondeterministic polynomial time complexity class using nanoscale vanadium-dioxide-based oscillators integrated onto a Silicon platform. Specifically, we demonstrate how the dynamic behavior of coupled vanadium dioxide devices can effectively solve combinatorial optimization problems, including Graph Coloring, Max-cut, and Max-3SAT problems. The electrical mappings of these problems are derived from the equivalent Ising Hamiltonian formulation to design circuits with up to nine crossbar vanadium dioxide oscillators. Using sub-harmonic injection locking techniques, we binarize the solution space provided by the oscillators and demonstrate that graphs with high connection density (η > 0.4) converge more easily towards the optimal solution due to the small spectral radius of the problem’s equivalent adjacency matrix. Our findings indicate that these systems achieve stability within 25 oscillation cycles and exhibit power efficiency and potential for scaling that surpasses available commercial options and other technologies under study. These results pave the way for accelerated parallel computing enabled by large-scale networks of interconnected oscillators.
In-situ low-temperature sulfur CVD on metal sulfides with SO2 to realize self-sustained adsorption of mercury
Authors: Hong Qinyuan , Xu Haomiao , Sun Xiaoming , Li Jiaxing , Huang Wenjun , Qu Zan , Zhang Lizhi , Yan Naiqiang
Published: April 2024

Capturing gaseous mercury (Hg0) from sulfur dioxide (SO2)-containing flue gases remains a common yet persistently challenge. Here we introduce a low-temperature sulfur chemical vapor deposition (S-CVD) technique that effectively converts SO2, with intermittently introduced H2S, into deposited sulfur (Sd0) on metal sulfides (MS), facilitating self-sustained adsorption of Hg0. ZnS, as a representative MS model, undergoes a decrease in the coordination number of Zn–S from 3.9 to 3.5 after Sd0 deposition, accompanied by the generation of unsaturated-coordinated polysulfide species (Sn2–, named Sd*) with significantly enhanced Hg0 adsorption performance. Surprisingly, the adsorption product, HgS (ZnS@HgS), can serve as a fresh interface for the activation of Sd0 to Sd* through the S-CVD method, thereby achieving a self-sustained Hg0 adsorption capacity exceeding 300 mg g−1 without saturation limitations. Theoretical calculations substantiate the self-sustained adsorption mechanism that S8 ring on both ZnS and ZnS@HgS can be activated to chemical bond S4 chain, exhibiting a stronger Hg0 adsorption energy than pristine ones. Importantly, this S-CVD strategy is applicable to the in-situ activation of synthetic or natural MS containing chalcophile metal elements for Hg0 removal and also holds potential applications for various purposes requiring MS adsorbents.
Fouling-resistant reverse osmosis membranes grafted with 2-aminoethanethiol having a low interaction energy with charged foulants
Authors: Xiao Jun , Hao Shuang , Qin Yiwen , Qi Pengfei , Zhang Zhaoqian , Hu Yunxia
Published: April 2024

Many fouling-resistant materials have been grafted or coated on the RO membrane surface for fouling-resistance. However, these modified RO membranes still exhibit a fast flux drop towards small charged organic foulants. Herein, we creatively use the quantum chemistry method to screen the thiol group having a close to zero interaction energy with small charged organic foulants. Thus, we selected a small molecule of 2-aminoethanethiol (AET) having a fouling-resistant thiol group and a reactive amine group for RO membrane surface modification. The water permeance of the AET-grafted RO membrane increases from 2.6 ± 0.1 L m−2 h−1 bar−1 to 3.2 ± 0.05 L m−2 h−1 bar−1, 23% higher than that of the pristine membrane. Moreover, the AET-grafted RO membrane exhibits excellent fouling resistance against charged surfactants. Our study offers insights on the design of fouling-resistant molecules for antifouling surface modification of RO membranes towards small charged organic foulants.
Largest known madtsoiid snake from warm Eocene period of India suggests intercontinental Gondwana dispersal
Authors: Datta Debajit , Bajpai Sunil
Published: April 2024

Here we report the discovery of fossils representing partial vertebral column of a giant madtsoiid snake from an early Middle Eocene (Lutetian, ~ 47 Ma) lignite-bearing succession in Kutch, western India. The estimated body length of ~ 11–15 m makes this new taxon (Vasuki indicus gen et sp. nov.) the largest known madtsoiid snake, which thrived during a warm geological interval with average temperatures estimated at ~ 28 °C. Phylogenetically, Vasuki forms a distinct clade with the Indian Late Cretaceous taxon Madtsoia pisdurensis and the North African Late Eocene Gigantophis garstini. Biogeographic considerations, seen in conjunction with its inter-relationship with other Indian and North African madtsoiids, suggest that Vasuki represents a relic lineage that originated in India. Subsequent India-Asia collision at ~ 50 Ma led to intercontinental dispersal of this lineage from the subcontinent into North Africa through southern Eurasia.
Physicochemical stability of bortezomib solutions for subcutaneous administration
Authors: Gómez Ángela , Benéitez García Mª Cristina , Barrueco Nélida , Lucena-Campillo Mª Amparo , López-Lunar Elena , García-Díaz Benito , Vicario-de-la-Torre Marta , Escobar-Rodríguez Ismael , Gil-Alegre María Esther
Published: April 2024

For the majority of cytotoxic drug preparations, such as bortezomib, the unit dose information is not available. In addition, there is a lack of information on the physicochemical stability of the pharmaceutical preparation after opening; this information is crucial for its administration to patients in successive visits, and the per-patient cost can be affected. The purpose of our proposed physicochemical stability study is to determine the shelf life of the reconstituted liquid product under refrigeration and clinical practice conditions. This evaluation was extended to both vials and ready-to-use syringes prefilled with the contents of the open vial. The stability test design includes the specified storage conditions and the critical physicochemical parameters of reconstituted injectable bortezomib. Furthermore, this approach includes the determination of impurities, the monitoring of the purity of the mean peak using a photodiode array, the control of the mass balance, the monitoring of subvisible particles using a laser diffraction analyser, and the setting of stability specifications. For the chemical stability study, the amount of bortezomib and its degradation products were determined using a stability-indicating HPLC method. The physical inspection of the samples was performed throughout the stability study, and their pH values were also monitored. Bortezomib (2.5 mg/mL) in 0.9% sodium chloride remained stable for 7 days when stored in both polypropylene syringes and vials at 5 ± 3 °C (refrigeration) and shielded from light. Additionally, it exhibits stability for 24 h under storage conditions simulating clinical use (20–30 °C and protected from light). The proposed protocol provides the stability in the vials once reconstituted and in prefilled refrigerated syringes; this protocol can be used to reduce waste and increase cost savings.
A data-driven global flood forecasting system for medium to large rivers
Authors: Palash Wahid , Akanda Ali S. , Islam Shafiqul
Published: April 2024

Losses from catastrophic floods are driving intense efforts to increase preparedness and improve response to disastrous flood events by providing early warnings. Yet accurate flood forecasting remains a challenge due to uncertainty in modeling, calibrating, and validating a useful early warning system. This paper presents the Requisitely Simple (ReqSim) flood forecasting system that includes key variables and processes of basin hydrology and atmospheric forcing in a data-driven modeling framework. The simplicity of the modeling structure and data requirements of the system allows for customization and implementation in any medium to large rain-fed river basin globally, provided there are water level or discharge measurements at the forecast locations. The proposed system's efficacy is demonstrated in this paper through providing useful forecasts for various river basins around the world. This include 3–10-day forecasts for the Ganges and Brahmaputra rivers in South Asia, 2–3-day forecast for the Amur and Yangtze rivers in East Asia, 5–10-day forecasts for the Niger, Congo and Zambezi rivers in West and Central Africa, 6–8-day forecasts for the Danube River in Europe, 2–5-day forecasts for the Parana River in South America, and 2–7-day forecasts for the Mississippi, Missouri, Ohio, and Arkansas rivers in the USA. The study also quantifies the effect of basin size, topography, hydrometeorology, and river flow controls on forecast accuracy and lead times. Results indicate that ReqSim's forecasts perform better in river systems with moderate slopes, high flow persistence, and less flow controls. The simple structure, minimal data requirements, ease of operation, and useful operational accuracy make ReqSim an attractive option for effective real-time flood forecasting in medium and large river basins worldwide.
Ambulatory electrocardiographic longitudinal monitoring in a canine model for Duchenne muscular dystrophy identifies decreased very low frequency power as a hallmark of impaired heart rate variability
Authors: Barthélémy Inès , Su Jin Bo , Cauchois Xavier , Relaix Frédéric , Ghaleh Bijan , Blot Stéphane
Published: April 2024

Duchenne muscular dystrophy (DMD) patients exhibit a late left ventricular systolic dysfunction preceded by an occult phase, during which myocardial fibrosis progresses and some early functional impairments can be detected. These latter include electrocardiographic (ECG) and heart rate variability (HRV) abnormalities. This longitudinal study aimed at describing the sequence of ECG and HRV abnormalities, using Holter ECG in the GRMD (Golden retriever muscular dystrophy) dog model, known to develop a DMD-like disease, including cardiomyopathy. Most of the known ECG abnormalities described in DMD patients were also found in GRMD dogs, including increased heart rate, prolonged QT and shortened PR intervals, ventricular arrhythmias, and several of them could be detected months before the decrease of fractional shortening. The HRV was impaired like in DMD patients, one of the earliest evidenced abnormalities being a decrease in the very low frequency (VLF) component of the power spectrum. This decrease was correlated with the further reduction of fractional shortening. Such decreased VLF probably reflects impaired autonomic function and abnormal vasomotor tone. This study provides new insights into the knowledge of the GRMD dog model and DMD cardiomyopathy and emphasizes the interest to monitor the VLF power in DMD patients, still unexplored in this disease, whilst it is highly predictive of deleterious clinical events in many other pathological conditions.
Somatic mutations of esophageal adenocarcinoma: a comparison between Black and White patients
Authors: Lim Hyeyeun , Gingras Marie-Claude , Zhao Jing , Byun Jinyoung , Castro Patricia D. , Tsavachidis Spiridon , Hu Jianhong , Doddapaneni Harshavardhan , Han Yi , Muzny Donna M. , Gibbs Richard A. , Amos Christopher I. , Thrift Aaron P.
Published: April 2024

Esophageal adenocarcinoma is the most common histological subtype of esophageal cancer in Western countries and shows poor prognosis with rapid growth. EAC is characterized by a strong male predominance and racial disparity. EAC is up to fivefold more common among Whites than Blacks, yet Black patients with EAC have poorer survival rates. The racial disparity remains largely unknown, and there is limited knowledge of mutations in EAC regarding racial disparities. We used whole-exome sequencing to show somatic mutation profiles derived from tumor samples from 18 EAC male patients. We identified three molecular subgroups based on the pre-defined esophageal cancer-specific mutational signatures. Group 1 is associated with age and NTHL1 deficiency-related signatures. Group 2 occurs primarily in Black patients and is associated with signatures related to DNA damage from oxidative stress and NTHL1 deficiency-related signatures. Group 3 is associated with defective homologous recombination-based DNA often caused by BRCA mutation in White patients. We observed significantly mutated race related genes (LCE2B in Black, SDR39U1 in White) were (q-value < 0.1). Our findings underscore the possibility of distinct molecular mutation patterns in EAC among different races. Further studies are needed to validate our findings, which could contribute to precision medicine in EAC.
Genetic architecture of white striping in turkeys (Meleagris gallopavo)
Authors: Vanderhout Ryley J. , Abdalla Emhimad A. , Leishman Emily M. , Barbut Shai , Wood Benjamin J. , Baes Christine F.
Published: April 2024

White striping (WS) is a myopathy of growing concern to the turkey industry. It is rising in prevalence and has negative consequences for consumer acceptance and the functional properties of turkey meat. The objective of this study was to conduct a genome-wide association study (GWAS) and functional analysis on WS severity. Phenotypic data consisted of white striping scored on turkey breast fillets (N = 8422) by trained observers on a 0–3 scale (none to severe). Of the phenotyped birds, 4667 genotypic records were available using a proprietary 65 K single nucleotide polymorphism (SNP) chip. The SNP effects were estimated using a linear mixed model with a 30-SNP sliding window approach used to express the percentage genetic variance explained. Positional candidate genes were those located within 50 kb of the top 1% of SNP windows explaining the most genetic variance. Of the 95 positional candidate genes, seven were further classified as functional candidate genes because of their association with both a significant gene ontology and molecular function term. The results of the GWAS emphasize the polygenic nature of the trait with no specific genomic region contributing a large portion to the overall genetic variance. Significant pathways relating to growth, muscle development, collagen formation, circulatory system development, cell response to stimulus, and cytokine production were identified. These results help to support published biological associations between WS and hypoxia and oxidative stress and provide information that may be useful for future-omics studies in understanding the biological associations with WS development in turkeys.
Temporal dynamics of public transportation ridership in Seoul before, during, and after COVID-19 from urban resilience perspective
Authors: Lee Sangwan , Kim Jooae , Cho Kuk
Published: April 2024

We delve into the temporal dynamics of public transportation (PT) ridership in Seoul, South Korea, navigating the periods before, during, and after the COVID-19 pandemic through a spatial difference-in-difference model (SDID). Rooted in urban resilience theory, the study employs micro-level public transportation card data spanning January 2019 to December 2023. Major findings indicate a substantial ridership decline during the severe COVID impact phase, followed by a period in the stable and post-COVID phases. Specifically, compared to the pre-COVID phase, PT ridership experienced a 32.1% decrease in Severe, followed by a reduced magnitude of 21.8% in Stable and 13.5% in post-COVID phase. Interestingly, the observed decrease implies a certain level of adaptability, preventing a complete collapse. Also, contrasting with findings in previous literature, our study reveals a less severe impact, with reductions ranging from 27.0 to 34.9%. Moreover, while the ridership in the post-COVID phase exhibits recovery, the ratio (Post/Pre) staying below 1.0 suggests that the system has not fully returned to its pre-pandemic state. This study contributes to the urban resilience discourse, illustrating how PT system adjusts to COVID, offering insights for transportation planning.
Convolutional neural networks develop major organizational principles of early visual cortex when enhanced with retinal sampling
Authors: da Costa Danny , Kornemann Lukas , Goebel Rainer , Senden Mario
Published: April 2024

Primate visual cortex exhibits key organizational principles: cortical magnification, eccentricity-dependent receptive field size and spatial frequency tuning as well as radial bias. We provide compelling evidence that these principles arise from the interplay of the non-uniform distribution of retinal ganglion cells, and a quasi-uniform convergence rate from the retina to the cortex. We show that convolutional neural networks outfitted with a retinal sampling layer, which resamples images according to retinal ganglion cell density, develop these organizational principles. Surprisingly, our results indicate that radial bias is spatial-frequency dependent and only manifests for high spatial frequencies. For low spatial frequencies, the bias shifts towards orthogonal orientations. These findings introduce a novel hypothesis about the origin of radial bias. Quasi-uniform convergence limits the range of spatial frequencies (in retinal space) that can be resolved, while retinal sampling determines the spatial frequency content throughout the retina.
Statine-based peptidomimetic compounds as inhibitors for SARS-CoV-2 main protease (SARS-CoV‑2 Mpro)
Authors: Azevedo Pedro Henrique R. de A. , Camargo Priscila G. , Constant Larissa E. C. , Costa Stephany da S. , Silva Celimar Sinézia , Rosa Alice S. , Souza Daniel D. C. , Tucci Amanda R. , Ferreira Vivian N. S. , Oliveira Thamara Kelcya F. , Borba Nathalia R. R. , Rodrigues Carlos R. , Albuquerque Magaly G. , Dias Luiza R. S. , Garrett Rafael , Miranda Milene D. , Allonso Diego , Lima Camilo Henrique da S. , Muri Estela Maris F.
Published: April 2024

COVID-19 is a multisystemic disease caused by the SARS-CoV-2 airborne virus, a member of the Coronaviridae family. It has a positive sense single-stranded RNA genome and encodes two non-structural proteins through viral cysteine-proteases processing. Blocking this step is crucial to control virus replication. In this work, we reported the synthesis of 23 statine-based peptidomimetics to determine their ability to inhibit the main protease (Mpro) activity of SARS-CoV-2. Among the 23 peptidomimetics, 15 compounds effectively inhibited Mpro activity by 50% or more, while three compounds (7d, 8e, and 9g) exhibited maximum inhibition above 70% and IC50 < 1 µM. Compounds 7d, 8e, and 9g inhibited roughly 80% of SARS-CoV-2 replication and proved no cytotoxicity. Molecular docking simulations show putative hydrogen bond and hydrophobic interactions between specific amino acids and these inhibitors. Molecular dynamics simulations further confirmed the stability and persisting interactions in Mpro's subsites, exhibiting favorable free energy binding (ΔGbind) values. These findings suggest the statine-based peptidomimetics as potential therapeutic agents against SARS-CoV-2 by targeting Mpro.
Imaging the top of the Earth’s inner core: a present-day flow model
Authors: Tkalčić Hrvoje , Belonoshko Anatoly B. , Muir Jack B. , Mattesini Maurizio , Moresi Louis , Waszek Lauren
Published: April 2024

Despite considerable progress in seismology, mineral physics, geodynamics, paleomagnetism, and mathematical geophysics, Earth’s inner core structure and evolution remain enigmatic. One of the most significant issues is its thermal history and the current thermal state. Several hypotheses involving a thermally-convecting inner core have been proposed: a simple, high-viscosity, translational mode, or a classical, lower-viscosity, plume-style convection. Here, we use state-of-the-art seismic imaging to probe the outermost shell of the inner core for its isotropic compressional speed and compare it with recently developed attenuation maps. The pattern emerging in the resulting tomograms is interpreted with recent data on the viscosity of iron as the inner core surface manifestation of a thermally-driven flow, with a positive correlation among compressional speed and attenuation and temperature. Although the outer-core convection controls the heat flux across the inner core boundary, the internally driven inner-core convection is a plausible model that explains a range of observations for the inner core, including distinct anisotropy in the innermost inner core.
Adaptive immune responses to two-dose COVID-19 vaccine series in healthy Canadian adults ≥ 50 years: a prospective, observational cohort study
Authors: Gaultier Gabrielle N. , McMillan Brynn , Poloni Chad , Lo Mandy , Cai Bing , Zheng Jean J. , Baer Hannah M. , Shulha Hennady P. , Simmons Karen , Márquez Ana Citlali , Bartlett Sofia R. , Cook Laura , Levings Megan K. , Steiner Theodore , Sekirov Inna , Zlosnik James E. A. , Morshed Muhammad , Skowronski Danuta M. , Krajden Mel , Jassem Agatha N. , Sadarangani Manish
Published: April 2024

To evaluate immune responses to COVID-19 vaccines in adults aged 50 years and older, spike protein (S)-specific antibody concentration, avidity, and function (via angiotensin-converting enzyme 2 (ACE2) inhibition surrogate neutralization and antibody dependent cellular phagocytosis (ADCP)), as well as S-specific T cells were quantified via activation induced marker (AIM) assay in response to two-dose series. Eighty-four adults were vaccinated with either: mRNA/mRNA (mRNA-1273 and/or BNT162b2); ChAdOx1-S/mRNA; or ChAdOx1-S/ChAdOx1-S. Anti-S IgG concentrations, ADCP scores and ACE2 inhibiting antibody concentrations were highest at one-month post-second dose and declined by four-months post-second dose for all groups. mRNA/mRNA and ChAdOx1-S/mRNA schedules had significantly higher antibody responses than ChAdOx1-S/ChAdOx1-S. CD8+ T-cell responses one-month post-second dose were associated with increased ACE2 surrogate neutralization. Antibody avidity (total relative avidity index) did not change between one-month and four-months post-second dose and did not significantly differ between groups by four-months post-second dose. In determining COVID-19 correlates of protection, a measure that considers both antibody concentration and avidity should be considered.