bluuebunny/crossref_metadata_2025 huggingface dataset:
The Potential for the Development of Critical Thinking Tests : An Overview of Educators' Perceptions
Authors: Hidayati Nurkhairo , Suryanti Suryanti , Cahaya Nilam
Published: March 2023

This study aimed to investigate teachers' perceptions about the need to develop critical thinking tests. The research method used quantitative descriptive. Research involved 20 biology teachers who taught at the senior high school in Riau. The data collection instrument was a questionnaire accompanied by a discussion with the teacher. The questionnaire contained questions regarding the teacher's knowledge of critical thinking, the availability of critical thinking instruments, and their implementation in learning. Data analysis was carried out in a quantitative descriptive. The results of the study show that the teachers’ knowledge of critical thinking is developing. The teacher also explained that it is crucial for students to have critical thinking skills so that measurements need to be taken. The availability of instruments to measure critical thinking when viewed from the percentage of teachers who provide them is 70%. In its implementation, the teacher makes critical thinking questions sourced from the question bank, articles, and books. The questions prepared by the teacher are also able to measure critical thinking even though they do not yet cover all aspects of critical thinking. Based on the research results, it can be recommended that the development of critical thinking tests can be carried out by the aspects of critical thinking (basic clarification, bases for a decision, inference, advanced clarification, supposition and integration, and strategies and tactics) and in line with the material being studied at school.
An Analysis of the Quality of Education based on Education Indicators in West Sumatra Province
Authors: Manik Yuni Mariani , Prasetyo Novi Eko , Sulistyo Sulistyo
Published: March 2023

This research aims to analyze the Net Enrollment Ratio (NER), the number of school educational facilities at different levels of education, and the student-teacher ratio in West Sumatera Province. This research method used a descriptive with a quantitative approach and secondary data from Central Agency on Statistics for the period 2019-2021. The analytical technique used in this research is descriptive quantitative data analysis. The results of the analysis show that the Net Enrollment Ratio of each age group experienced an increase of 0.09-0.044%. The number of elementary schools in West Sumatera Province has fluctuated while Madrasah Ibtidaiyah has increased. The number of junior high school has increased, while the number of Madrasah Tsanawiyah has decreased. The number of upper-level education is dominated by senior high school, followed by Madrasah Aliyah, then vocational high school. The trend of changes in the elementary school student-teacher ratio has decreased, meanwhile both junior and senior high school teachers have increased. In general, the student-teacher ratio in Sumatera Barat is fairly good because it does not exceed the standards set by the government.
Education in Indonesia (Merdeka Curriculum) and Japan Curriculum : What’s the Difference?
Authors: Prakoso Albrian Fiky , Andriansyah Eka Hendi , Rafsanjani Mohamad Arief , Nurlaili Eka Indah , Arif Amirul
Published: March 2023

The purpose of this study is analyze of the differences in education between Indonesia, which has begun to develop an independent curriculum, and education in Japan. This study used the systematic literature review method. The population consists of 99 articles that discuss education in Indonesia and Japan, and were published between 2010 and 2022. From 99 articles, 29 articles were sampled. This study used documentation instruments in the form of articles published with criteria for selecting these 29 articles as samples, which were articles published in nationally accredited journals, indexed proceedings, and Scopus-indexed international journals and then analyzed using thematic analysis. The results obtained are that the  significant fundamental differences in education in Japan and Indonesia lie in international education, character education, ethics and discipline and fields of study. The implication of this research is that in the development of a new curriculum, elements of culture and values are still prioritized as the characteristics and profile characteristics of graduates.
Development of PowToon Animation Video on Joyful Learning Loaded Reaction Rate Material to Increase High School Students' Learning Motivation
Authors: Tafani Tafani , Kamaludin Agus
Published: March 2023

This research aims to produce animated PowToon videos on reaction rate material with Joyful Learning content and to analyze the quality of the product being developed. The method used in this study used a 4-D model consisting of define, design, development, and disseminate stages, but was limited to the developing stage. The instrument used in the study was in the form of video quality assessment sheets and student responses. The data were analyzed descriptively and quantitatively. The results of video evaluation by media experts, material experts, reviewers, and student responses were 90%, 95%, 93%, and 92%, which were included in the very good category. That animated video had the advantage of being fun for students because it could present factual information through engaging animations and learning experiences that are difficult for students to get outside the school environment. Based on the study results, the PowToon animation video on the developed reaction rate material can be used as an alternative learning medium in creating fun learning and increasing student motivation.
Examining Academic Self Efficacy to Achieve Student Academic Performance: Evidence from Computer-Based Training
Authors: Palapa Arika
Published: March 2023

This study aims to investigate the impact of mastery goal orientation (MGO) and self-directed learning (SDL) on academic performance via academic self-efficacy (ASE). As a learning medium, computer-based training (CBT) attempts to boost student confidence. The study used a quantitative approach, with 142 merchant marine school students serving as participants. The questionnaire was used as a research tool to collect data. To assess and measure variable relationships, research data was examined using structural equation modeling. According to the findings of the study, MGO and SDL have a direct impact on ASE and academic achievement. The study revealed that ASE mediated MGO and SDL in relation to academic achievement. Individual ambition to acquire information and competence results in ASE, which indicated students' confidence. CBT allows students to broaden their learning interests. SDL and CBT complement each other in a mutually beneficial way. CBT is a learning tool; nevertheless, without SDL, it will be ineffective in improving academic achievement. SDL without CBT is less effective since students are unable to explore a broader range of information and expertise. When students use CBT as a learning medium, they frequently study and practice, gaining more knowledge and expertise. CBT is thought to be an effective learning medium for increasing student academic confidence and performance.
Developing Basic Accounting E-Module Based on Scientific Approach in Vocational High Schools
Authors: Fahrurrozi Muh , Mohzana Mohzana , Mispandi Mispandi , Murcahyanto Hary
Published: March 2023

This research aims to produce a basic accounting economics e-module based on a scientific approach to increase learning motivation and understanding for students of Vocational High Schools. In addition, this study aims to analyze the feasibility and effectiveness of basic accounting e-modules based on a scientific approach developed based on the results of expert design, material, and student responses. This research applied the Thiagarajan 4-D model through the following steps: Define, Design, Develop, and Disseminate. Two model instruments were implemented upon expert judgements; assessment form of content (material) expert and assessment from media expert. To display, all data collected was processed qualitatively and quantitatively. It involved student responses and numerical data (via questionnaires and validation forms) from both experts. The results showed that (1) the development of the Basic Accounting E-Module Based on the Scientific Approach in vocational high school grade X implements the 4D model up to the development stage, (2) the assessment from media design experts obtained is 91%, which means '"very feasible" and the material expert assessment obtained is 92% which means '"very feasible," (3) The results of the responses of class X students of vocational high school in limited trials obtained an average score of 84% with the category "Very Feasible" and in the field, tests obtained an average overall score of 94% with the category "Very feasible." The module can widely impact better content of teaching basic accounting as a measurable guide for prospective school teachers.
Integrity of Discourse in the Radar Sulawesi Barat Daily Newspaper : The Use of Cohesion and Coherence in Learning Indonesian to Improve Students' Literacy and Knowledge Skills
Authors: Idris Idris , Fandi Al , Tahir Moh , Suputra Gusti Ketut Alit , Krida Syamsuddin
Published: March 2023

This study aims to analyze cohesion and coherence in forming the wholeness of the discourse contained in the Radar Sulawesi Barat daily. This research was qualitative. The data was collected using listening and note-taking techniques with several stages: reading, marking, and classifying. The data obtained in this study were written as elements that made up the wholeness of the discourse. The method and technique of data analysis used was the intralingual equivalent method with the comparison technique of equating the main points. Based on the study's results, cohesion and coherence shape the wholeness of discourse in the Radar Sulawesi Barat daily. Discourse cohesion found in this study was a marker of grammatical and lexical cohesion. Grammatical cohesion markers include (1) references, (2) substitutions, and (3) conjunctions. Lexical cohesion markers are repetition; the repetition obtained consists of (1) complete repetition, (2) repetition with changes in form, (3) repetition with changes, (4) repetition with synonym changes, and (5) repetition with hyponyms. In addition to repetition, a marker of lexical cohesion was also found, namely collocation. Furthermore, the element that forms the wholeness of discourse was coherence. This study had a meaning or semantic relationship between elements or sentences in the discourse, which can be used as a strategy for learning Indonesian.
The Extent of Silicone Oil Emulsification Revealed by Ultra-wide-field Fundus Photography and Optical Coherence Tomography
Authors: Hong Rim Kyung , Kim Du Roo , Hong Eun Hee , Kang Min Ho , Shin Yong Un , Cho Heeyoon
Published: March 2023

Purpose: We assessed the extent of silicone oil (SO) emulsification using ultra-wide-field fundus photography (wFP) and the reflective ratios of swept-source optical coherence tomography (SS-OCT).Methods: We retrospectively enrolled 51 eyes of 51 patients who underwent intravitreal SO tamponade with vitrectomy. Two weeks after SO tamponade and immediately before SO removal, ultra-wide-field fundus photography and SS-OCT were performed. Based on the numbers of emulsified droplets in the ultra-wide-field fundus photographs, SO emulsification was qualitatively graded from 0 to 4. Reflective ratios were calculated by dividing the OCT reflectivity of the optic cup by the OCT reflectivity of SO near the retinal surface. We analyzed the changes in the SO emulsification grade and the reflective ratio over time (RR2/RR1).Results: The SO emulsification grade revealed by ultra-wide-field fundus photography was 2.12 ± 1.29, and the mean SS-OCT RR2/RR1 value was 1.14 ± 0.22. A longer duration of SO tamponade was associated with a higher emulsification grade on ultra-wide-field fundus photography and an increase in the RR2/RR1 value (both p < 0.01). We found a significant correlation between the SO emulsification grade on ultra-wide-field fundus photography and the SS-OCT RR2/RR1 (p = 0.028).Conclusions: Ultra-wide-field fundus photography and SS-OCT can be used to determine objectively the extent of SO emulsification; this may indicate the appropriate SO removal time any complication.
Two Cases of Paraproteinemic Keratopathy
Authors: Yun Hee Jee , Won Yeo Kyoung , Shin Eun Hye , Chung Tae-Young
Published: March 2023

Purpose: To report two cases of paraproteinemic keratopathy (PK).Case summary: A 57-year-old man visited the ophthalmology clinic complaining of decreased visual acuity since 1 year earlier. White crystalline deposits were observed on both corneas and the best corrected visual acuity (BCVA) of both eyes was 1.0. After 3 months, the BCVA decreased to 0.8 in the right and 0.6 in the left. Increased corneal deposits were observed. To rule out PK, his medical records were checked. The patient had been diagnosed with lymphoplasmacytic lymphoma 3 years earlier. Diagnosing PK, the patient was referred back to the Hemato-Oncology department. After 3 months of chemotherapy, the corneal deposits decreased and BCVA returned to 1.0. A 69-year-old woman presented complaining of decreased visual acuity for 2 years. The uncorrected visual acuity (UCVA) was 0.4 in the right and finger count at 30 cm in the left. Brown deposits were observed on both corneas and lenses. The serum copper level was elevated. She was referred to the internal medicine department and diagnosed with multiple myeloma. After treatment, the laboratory results returned to normal, but the UCVA decreased to hand motion in the left. A left white cataract was noted and cataract surgery was performed. 1 month postoperatively, the BCVA was 0.9, with no change in the corneal deposits.Conclusions: When there are corneal deposits, we should consider PK in the differential diagnosis. In such cases, systemic treatment is the first line. Therefore, diagnosing and referring PK patients to the internal medicine department is crucial.
Müziği Konumlandıran Felsefe Algısından Felsefeye Çığır Açan Bir Müzik Anlayışına
Authors: DOĞRAMACI Halil İbrahim , YILDIZ Ömer
Published: March 2023

Felsefe ile müzik arasındaki ilişki felsefe tarihi boyunca farklı görünümler kazanmıştır. Biri diğerine indirgenemez karakterdeki bu iki farklı varoluş tarzının nasıl bir ilişki içinde olduğu sorusu da bizzat bu ilişkinin kendisindeki değişkenlik sebebiyle önemini daima korumuştur. Bu yüzden bu sorunun diğer bütün felsefi sorular gibi, belirli bir felsefi ekol ya da filozofun görüşleri lehine cevaplanarak karara bağlanabilir bir karakteri yoktur. Aksine o, felsefe tarihinin farklı dönemlerindeki perspektifler ortaya çıkarıldıkça farklı cevaplara imkân tanıyan bir istifham olmak durumundadır. Bu makalede bu sorunun cevabı aranırken bir zemin teşkil etmesi amacıyla öncelikle, Antik Yunan felsefesinin ve Klasik İslam felsefesinin müziğe yaklaşımları kısaca ele alınmıştır. Bir sınırlılık olarak Antik Yunan filozoflarından Pisagor, Platon ve Aristoteles’e; Klasik İslam filozoflarından ise Kindî, Fârâbî ve İbn Sînâ’ya referansta bulunulmuş, ardından felsefenin, kendi varoluş tarzından apayrı bir varoluş tarzına sahip olan müziği konumlandırma çabası sorunsallaştırılarak bu sorun “yer”, mekân”, “konumlandırma”, “iktidar”, “yabancılaşma” ve özellikle de “tevazu” kavramı ışığında irdelenmiştir. Felsefe ve müziğin ilişkiselliği vurgulanırken başlıkta dikkat çekilen “çığır açma” deyimi gündelik kullanımından çıkartılarak makalenin kendi felsefi gayesi lehinde kavramsallaştırılmıştır.
Análise dos riscos e prevenções da cubagem de espécies florestais da Amazônia Oriental Brasileira
Authors: Oliveira Williams Dias de , Carneiro Francimary da Silva , Bassi Natália Ribeiro , Oliveira Tamires Borges de , Oliveira Sonia Telma Silva , Maia Thayse Evanuele de Jesus Cordeiro , Cordeiro Denise de Fátima de Jesus , Frazão Alex da Silva , Pinheiro Klewton Adriano Oliveira
Published: March 2023

Neste trabalho foi feito um estudo sobre os riscos associados à atividade de cubagem de toras e resíduos florestal nas florestas do Estado do Pará, buscou-se identificar os riscos de acidente no trabalho e suas prevenções que podem ser elencadas sobre esta atividade através de consulta bibliográfica e atividades laborais. Como resultado obteve-se os seguintes fatores para a prevenção de acidentes no trabalho: medidas de controle, obedecer à época correta das atividades e o uso de Equipamentos de proteção Individual - EPI e Equipamento de Proteção Coletivo - EPC.
Recobrimento radicular em recessões dentais unitárias utilizando matriz colágena Mucograft® comparadas ao enxerto autógeno: revisão de literatura
Authors: Matos Flávia Gomes , Moura Guilherme Bianchine de
Published: March 2023

O tratamento de recessões gengivais além de proporcionar maior conforto ao paciente diminuindo a sensibilidade restabelece o posicionamento normal do tecido gengival melhorando a estética. Procedimentos cirúrgicos rápidos e com menor sintomatologia dolorosa seriam ideais na prática clínica para o recobrimento radicular. Dessa forma, biomateriais substitutos a enxertos autógenos estão cada vez mais sendo pesquisados. Assim, o objetivo deste estudo é verificar sob revisão da literatura científica atualizada, artigos clínicos que realizaram o recobrimento radicular em recessões dentais unitárias utilizando do Mucograft® comparativamente aos enxertos autógenos. Para isso, o termo Mucograft® foi utilizado para buscas no Pubmed e Google e 48 resultados foram obtidos. Desses, 9 artigos foram selecionados. 6 estudos avaliaram a porcentagem de recobrimento radicular, enquanto que 2 avaliaram o aumento da faixa de tecido queratinizado e 1 as mudanças histológicas. O uso de Mucograft® demonstrou resultados positivos em relação a capacidade de recobrimento radicular com boa estética e nível de dor pós operatória semelhante ao do obtido pelo enxerto autógeno. As evidências científicas demonstram que o a Matriz Mucograft® é um material promissor, mas comprovações com maior tempo de acompanhamento são necessárias para auxiliar o clínico nas suas escolhas.
Estágio supervisionado em tempos de Covid-19: narrativas e reflexões no contexto do ensino remoto
Authors: Moura Francisco Kássio Teixeira de
Published: March 2023

O estágio supervisionado como um componente curricular obrigatório nos cursos de licenciaturas é muito importante para formação inicial. O objetivo deste artigo consiste em destacar as contribuições, vivências e reflexões do estágio supervisionado remoto para formação inicial na escola de campo durante a pandemia de Covid-19, refletindo através das narrativas vividas. O presente artigo consiste em uma pesquisa de cunho qualitativa narrativa, essas narrativas em questão são das vivências e experiências do autor durante o estágio remoto no curso de Licenciatura em Ciências Biológicas, uma pesquisaformação realizada através da observação, os dados foram produzidos de forma descritiva, O campo da pesquisa foi uma Escola Pública Técnica da Rede Federal de Ensino, localizada em Teresina-PI, o estágio foi desenvolvido em uma turma do Primeiro ano do Ensino Médio na disciplina de Ciências Biológicas, os resultados narrados demonstram que as aulas remota propocionaram situações inovadoras, houve uma baixa adesão dos alunos as aulas remotas, os desafios e as situações imprevitas foram superados, diante disso fica claro que o estágio contribui para formação inicial e diante do cenário de pandemia o ensino remoto foi a única alternativa viável.
Epigenetic regulation of normal and aberrant myelopoiesis
Authors:   , Govers Anita Maria Antoinette Petronella
Published: March 2023

In the last two decades it has become increasingly evident that epigenetic changes are a contributing factor in aberrant myelopoiesis. Knowledge about epigenetic regulation of normal hematopoiesis is far from complete, however the use of chromatin modifying drugs has rapidly increased.
Work in this thesis is aimed to increase understanding of the effects of epigenetic regulation and epigenetic modifiers on both normal and aberrant hematopoiesis.
In Chapter 2, we investigated the role of acetylation of the transcription factor C/EPB in myelopoiesis. We found that acetylation of C/EBP is a prerequisite for normal neutrophil development. In Chapter 3, we investigated the effect of HDACi on megakaryocyte development. We found that manipulation of promotor acetylation by several HDACi (VPA and NAM) affected megakaryocyte development. Chapter 4 describes the effects of HDACi on normal hematopoiesis and especially neutrophil development. We investigated the transcriptomic, epigenomic, functional and morphological effects of two distinct HDACi, MS-275 and SAHA, in neutrophil development, providing insight into the regulatory mechanisms of HDACi. In Chapter 5, we performed a compound screen against epigenetic regulators to identify novel epigenetic drugs for the treatment of three types of pediatric AML; MLL-rearranged AML, CBF-arranged AML and acute megakaryoblastic AML in patients with Down syndrome (DS-AMKL). This identified potential therapeutic targets that could be of use in multiple pediatric AML subtypes. In Chapter 6, we have investigated the use of induced pluripotent stem cells (iPS) as an alternative source of human MSC, aiming to establish a standardised and easily replenishable source of MSC. Unfortunately, iPS-MSC did not prove to be a reproducible source of MSC for experimental use. Finally, Chapter 7, summarises the results and suggests future research that could address remaining and evolved questions from the work described in this thesis.
Taken together, this thesis provides novel insights into the epigenetic of regulation of normal and malignant hematopoiesis. This knowledge can contribute to the development of novel epigenetic drugs for the treatment of myeloid malignancies.
Risks and Safety in Paediatric Radiology
Authors: Koutsouki Evgenia
Published: March 2023

In a highly engaging session at the European Congress of Radiology (ECR)in Vienna, Austria, which took place 1st–5th March 2023 and was chaired by Hans-Joachim Mentzel, experts came together to discuss risks and safety measures, including different radiology practices in children.
The Role of Granulocyte-Colony Stimulating Factor Biosimilars for Supportive Cancer Care: A Year in Review
Authors: Moir Hannah
Published: March 2023

This year-in-review article provides insights into clinical updates relating to granulocyte-colony stimulating factor (G-CSF) biosimilars research presented at five key congresses in 2022. These include the American Society of Clinical Oncology (ASCO) 55th Annual Meeting (3rd–7th June 2022, Chicago, Illinois, USA), European Society for Medical Oncology (ESMO) Congress (9th–13th September 2022, Paris, France), ESMO Asia Congress (2nd–4th December 2022, Singapore), San Antonio Breast Cancer Symposium (SABCS; 6th–10th December 2022, Texas, USA), and American Society of Hematology (ASH) 64th Annual Meeting and Exposition (10th–13th December 2022, New Orleans, Louisiana, USA). Alongside reviewing the current research presented at these key congresses, with a focus on the use of G-CSF agents and biosimilars in patients undergoing treatment for breast, colorectal, and gynaecological cancers, this article provides an overview of current guidelines on the use of G-CSF in supportive cancer care to manage chemotherapy-induced febrile neutropenia and explores trends in G-CSF biosimilars research.
Why, When, and How? Optimising the Management of Patients with Severe Eosinophilic Asthma
Authors: Cahill Mia
Published: March 2023

The main objectives of this symposium were to explore the challenges faced when treating patients with severe eosinophilic asthma, to evaluate the key clinical assessments that are available for early disease recognition, and to discuss the latest personalised treatment options that could shape future management strategies. Prof Ian Pavord opened the symposium by introducing uncontrolled severe asthma, focussing on the challenges and unmet needs of patients. Prof Buhl then delved into the basics of eosinophilic asthma from a molecular and physiological point of view, discussing the clinical relevance and characterisation of eosinophilic patients. Prof Costello focussed on the key clinical assessments (diagnosis, adherence, and phenotyping) and management of patients. Prof Castro summarised the latest evidence from studies of mepolizumab, benralizumab, reslizumab, anti-interleukin (IL)-4, and anti-IL-13 therapies, and how this relates to clinical practice.
Failure Analysis of OLTC during Transition Resistor Test
Authors: Prabakaran T. , Wadhwani M. K. , Munshi Sumbul , Roy Himangshu , Pathak Saumitra
Published: March 2023

Emerging modern technologies and ever-changing demands compete with each other to bring new changes to the sociotechnical life of human beings. OLTC is one such equipment connected as an integral part of the transformer to change the turns ratio while the transformer is in energized condition, to adjust the output voltage. Changing times and technology brought many changes in the OLTC techniques, design and operation. Traditionally OLTC is a complex mechanical device, which has some deficiencies. OLTC design elements are checked during the transition resistor test. Thus, OLTC testing for transition resistors is a method to enhance the reliability of OLTC design. This paper discusses a typical failure case of OLTC during the transition resistor test conducted in CPRI.
Experimental Analysis of Ceramic Insulators with Different Profiles Under Very High Pollution Zone by Solid Layer Method
Authors: Agarwal Shyam , Babu V. Mohan , Gobinath G. , Pandian G.
Published: March 2023

The outdoor insulators in service are exposed to marine, agricultural waste and industrial pollution. These pollutants get deposited on the insulator surface and lead to flashovers under fog conditions. There has been an abnormal increase in pollution levels due to rapid industrialization in the past two decades. This exponential increase in pollution levels causes frequent flashovers of insulators, leading to the tripping of transmission lines consecutively may result in blackouts, which severely affects the reliability of the power systems. The ESDD and NSDD measurement on the insulator surface are critical parameters for determining the pollution severity levels. This paper discusses ESDD and NSDD measurement procedures. In this paper, field pollution severity has been simulated in the Artificial Pollution Lab. In addition, the Non-Soluble Deposit Density (NSDD) component of pollutants for designing the creepage distance of ceramic insulators under heavy pollution conditions has been emphasized.
Bekir Sıtkı Paşanın Tokat Mutasarrıflığı Dönemi İcraatlarına Karşı Şikâyetler
Authors: AYKUN İbrahim
Published: March 2023

Osmanlı Devleti’nin önemli şehirlerinden biri olan Tokat, XIX. yüzyılın sonlarına doğru yaşanan Ermeni olaylarında adından sıkça söz ettirmiştir. Ermeni olaylarının uluslararası bir mahiyet kazanması aynı zamanda Osmanlı Devleti üzerinde büyük devletlerin baskı kurmasına da neden olacaktır. Bu dönemde gerçekleşen hadiselerin en önemli merkezlerinden birisi de Tokat’tır. Yunanistan’ın Girit’i ilhak etme teşebbüsü, büyük devletlerin bunu kabul etmemesi ve Osmanlı Devleti’nin yanında yer aldıkları bir sırada yaşanan Tokat Ermeni olayları devleti güç durumda bırakmıştır. Osmanlı Devleti içine düştüğü zor şartlar karşısında ilk iş olarak Tokat Mutasarrıfı Nazım Paşa ile Polis Ser-Komiserini azledecektir. Dirayetli bir Mutasarrıf aranırken Bekir Sıtkı Paşa akıllara gelmiş ve Mutasarrıflığa atanmıştır. Çalışma azmi ile göreve başlayan Bekir Sıtkı Paşa Tokat’a hizmet etmek için faaliyette bulunmuş, hatta günümüzde hala çalışan Saat Kulesi de onun Mutasarrıflığı döneminde inşa edilmiştir. Ancak Bekir Sıtkı Paşa’nın Mutasarrıflık döneminde Tokat’ta bazı kişilerle ters düşmesi bir dizi şikâyetin ve soruşturmanın da konusu olacaktır. Nitekim Bekir Sıtkı Paşa bu şikâyetler karşısında kendisini aklamak için uğraşmak zorunda da kalmıştır. Diğer taraftan Sivas Valisiyle olan sorunları da bu uğraşını zorlaştırmıştır. Bu çalışma Bekir Sıtkı Paşa’nın Tokat Mutasarrıflığı döneminde kendisini aklama çalışmalarına odaklanmıştır. Çalışmamızın ana kaynağını arşiv belgeleri oluşturmaktadır. Ayrıca konuyla ilgili olarak yapılmış araştırma ve inceleme eserlerine de müracaat edilmiştir.
Miedo a hablar en público y aceptación psicológica: un protocolo de intervención mediante realidad virtual basado en la Terapia de Aceptación y Compromiso
Authors: Montesinos Marín Francisco , Calderón Christian , Lobato David
Published: March 2023

Se describe un protocolo de intervención de 5 sesiones basado en la Terapia de Aceptación y Compromiso (ACT) dirigido a estudiantes universitarios que presentan miedo a hablar en público (MHP) y promueve los procesos centrales de ACT para el fomento de la flexibilidad psicológica. Dichos procesos son abordados durante la intervención a través de un entrenamiento que combina la exposición en vivo o mediante realidad virtual (RV) desde la perspectiva de la aceptación psicológica. Se presentan los resultados de la aplicación de las dos modalidades del protocolo en sendas estudiantes universitarias que reflejaron reducciones clínicamente significativas del MHP y la ansiedad social. Se concluye que la adaptación de los métodos clínicos de ACT en el ámbito del MHP en combinación con la RV puede suponer un avance significativo en el abordaje de este problema en el ámbito académico.
A comparative study on various cell sources for constructing tissue-engineered meniscus
Authors: Zheng Rui , Song Daiying , Ding Yangfan , Sun Binbin , Lu Changrui , Mo Xiumei , Xu Hui , Liu Yu , Wu Jinglei
Published: March 2023

Injury to the meniscus is a common occurrence in the knee joint and its management remains a significant challenge in the clinic. Appropriate cell source is essential to cell-based tissue regeneration and cell therapy. Herein, three commonly used cell sources, namely, bone marrow mesenchymal stem cell (BMSC), adipose-derived stem cell (ADSC), and articular chondrocyte, were comparatively evaluated to determine their potential for engineered meniscus tissue in the absence of growth factor stimulus. Cells were seeded on electrospun nanofiber yarn scaffolds that share similar aligned fibrous configurations with native meniscus tissue for constructing meniscus tissue in vitro. Our results show that cells proliferated robustly along nanofiber yarns to form organized cell-scaffold constructs, which recapitulate the typical circumferential fiber bundles of native meniscus. Chondrocytes exhibited different proliferative characteristics and formed engineered tissues with distinct biochemical and biomechanical properties compared to BMSC and ADSC. Chondrocytes maintained good chondrogenesis gene expression profiles and produced significantly increased chondrogenic matrix and form mature cartilage-like tissue as revealed by typical cartilage lacunae. In contrast, stem cells underwent predominately fibroblastic differentiation and generated greater collagen, which contributes to improved tensile strengths of cell-scaffold constructs in comparison to the chondrocyte. ADSC showed greater proliferative activity and increased collagen production than BMSC. These findings indicate that chondrocytes are superior to stem cells for constructing chondrogenic tissues while the latter is feasible to form fibroblastic tissue. Combination of chondrocytes and stem cells might be a possible solution to construct fibrocartilage tissue and meniscus repair and regeneration.
A surface metal ion-modified 3D-printed Ti-6Al-4V implant with direct and immunoregulatory antibacterial and osteogenic activity
Authors: Wu Yipeng , Shi Xiangwen , Wang Jianjun , Li Yang , Wu Jiang , Jia Daqi , Bai Yan , Wu Xiaopei , Xu Yongqing
Published: March 2023

The high concentration of antibacterial metal ions may exhibit unavoidable toxicity to cells and normal tissues. The application of antibacterial metal ions to activate the immune response and induce macrophages to attack and phagocytose bacteria is a new antimicrobial strategy. Herein, 3D-printed Ti-6Al-4V implants modified by copper, and strontium ions combined with natural polymers were designed to treat implant-related infections and osseointegration disorders. The polymer-modified scaffolds rapidly released a large amount of copper and strontium ions. During the release process, copper ions were employed to promote the polarization of M1 macrophages, thus inducing a proinflammatory immune response to inhibit infection and achieve the immune antibacterial activity. Meanwhile, copper and strontium ions promoted the secretion of bone-promoting factors by macrophages, induced osteogenesis and showed immunomodulatory osteogenesis. This study proposed immunomodulatory strategies based on the immunological characteristics of target diseases and provided ideas for the design and synthesis of new immunoregulatory biomaterials.
A single-tube-braided stent for various airway structures
Authors: Tong Xin , Jiang Yongkang , Mo Fei , Sun Zhongqing , Wu Xiaojun , Li Yingtian
Published: March 2023

Background: Airway stent has been widely used in airway procedures. However, the metallic and silicone tubular stents are not customized designed for individual patients and cannot adapt to complicated obstruction structures. Other customized stents could not adapt to complex airway structures with easy and standardized manufacturing methods.

Object: This study aimed to design a series of novel stents with different shapes which can adapt to various airway structures, such as the “Y” shape structure at the tracheal carina, and to propose a standardized fabrication method to manufacture these customized stents in the same way.

Methods: We proposed a design strategy for the stents with different shapes and introduced a braiding method to prototype six types of single-tube-braided stents. Theoretical model was established to investigate the radial stiffness of the stents and deformation upon compression. We also characterized their mechanical properties by conducting compression tests and water tank tests. Finally, a series of benchtop experiments and ex vivo experiments were conducted to evaluate the functions of the stents.

Results: The theoretical model predicted similar results to the experimental results, and the proposed stents could bear a compression force of 5.79N. The results of water tank tests showed the stent was still functioning even if suffering from continuous water pressure at body temperature for a period of 30 days. The phantoms and ex-vivo experiments demonstrated that the proposed stents adapt well to different airway structures.

Conclusion: Our study offers a new perspective on the design of customized, adaptive, and easy-to-fabricate stents for airway stents which could meet the requirements of various airway illnesses.
Poly(I:C)-induced maternal immune activation causes elevated self-grooming in male rat offspring: Involvement of abnormal postpartum static nursing in dam
Authors: Lan Xing-Yu , Gu You-Yu , Li Ming-Juan , Song Tian-Jia , Zhai Fu-Jun , Zhang Yong , Zhan Jiang-Shan , Böckers Tobias M. , Yue Xiao-Nan , Wang Jia-Nan , Yuan Shuo , Jin Meng-Ying , Xie Yu-Fei , Dang Wan-Wen , Hong Hai-Heng , Guo Zi-Rui , Wang Xue-Wei , Zhang Rong
Published: March 2023

Introduction: Maternal immune activation (MIA) is closely related to the onset of autism-like behaviors in offspring, but the mechanism remains unclear. Maternal behaviors can influence offspring’s development and behaviors, as indicated in both human and animal studies. We hypothesized that abnormal maternal behaviors in MIA dams might be other factors leading to delayed development and abnormal behaviors in offspring.

Methods: To verify our hypothesis, we analyzed poly(I:C)-induced MIA dam’s postpartum maternal behavior and serum levels of several hormones related to maternal behavior. Pup’s developmental milestones and early social communication were recorded and evaluated in infancy. Other behavioral tests, including three-chamber test, self-grooming test, open field test, novel object recognition test, rotarod test and maximum grip test, were performed in adolescence of pups.

Results: Our results showed that MIA dams exhibit abnormal static nursing behavior but normal basic care and dynamic nursing behavior. The serum levels of testosterone and arginine vasopressin in MIA dams were significantly reduced compared with control dams. The developmental milestones, including pinna detachment, incisor eruption and eye opening, were significantly delayed in MIA offspring compared with control offspring, while the weight and early social communication showed no significant differences between the two groups. Behavioral tests performed in adolescence showed that only male MIA offspring display elevated self-grooming behaviors and reduced maximum grip.

Discussion: In conclusion, MIA dams display abnormal postpartum static nursing behavior concomitantly with reduced serum levels of testosterone and arginine vasopressin, possibly involving in the pathogenesis of delayed development and elevated self-grooming in male offspring. These findings hint that improving dam’s postpartum maternal behavior might be a potential regime to counteract delayed development and elevated self-grooming in male MIA offspring.
Proteomic analysis defines the interactome of telomerase in the protozoan parasite, Trypanosoma brucei
Authors: Davis Justin A. , Reyes Andres V. , Nitika  , Saha Arpita , Wolfgeher Donald J. , Xu Shou-Ling , Truman Andrew W. , Li Bibo , Chakrabarti Kausik
Published: March 2023

Telomerase is a ribonucleoprotein enzyme responsible for maintaining the telomeric end of the chromosome. The telomerase enzyme requires two main components to function: the telomerase reverse transcriptase (TERT) and the telomerase RNA (TR), which provides the template for telomeric DNA synthesis. TR is a long non-coding RNA, which forms the basis of a large structural scaffold upon which many accessory proteins can bind and form the complete telomerase holoenzyme. These accessory protein interactions are required for telomerase activity and regulation inside cells. The interacting partners of TERT have been well studied in yeast, human, and Tetrahymena models, but not in parasitic protozoa, including clinically relevant human parasites. Here, using the protozoan parasite, Trypanosoma brucei (T. brucei) as a model, we have identified the interactome of T. brucei TERT (TbTERT) using a mass spectrometry-based approach. We identified previously known and unknown interacting factors of TbTERT, highlighting unique features of T. brucei telomerase biology. These unique interactions with TbTERT, suggest mechanistic differences in telomere maintenance between T. brucei and other eukaryotes.
Integrated proteome sequencing, bulk RNA sequencing and single-cell RNA sequencing to identify potential biomarkers in different grades of intervertebral disc degeneration
Authors: Yang Xiao , Lu Yang , Zhou Hang , Jiang Hai-Tao , Chu Lei
Published: March 2023

Low back pain (LBP) is a prevalent health problem worldwide that affects over 80% of adults during their lifetime. Intervertebral disc degeneration (IDD) is a well-recognized leading cause of LBP. IDD is classified into five grades according to the Pfirrmann classification system. The purpose of this study was to identify potential biomarkers in different IDD grades through an integrated analysis of proteome sequencing (PRO-seq), bulk RNA sequencing (bRNA-seq) and single-cell RNA sequencing (scRNA-seq) data. Eight cases of grade I-IV IDD were obtained. Grades I and II were considered non-degenerative discs (relatively normal), whereas grades III and IV were considered degenerative discs. PRO-seq analysis was performed to identify differentially expressed proteins (DEPs) in various IDD grades. Variation analysis was performed on bRNA-seq data to differentiate expressed genes (DEGs) in normal and degenerated discs. In addition, scRNA-seq was performed to validate DEGs in degenerated and non-degenerated nucleus pulposus (NP). Machine learning (ML) algorithms were used to screen hub genes. The receiver operating characteristic (ROC) curve was used to validate the efficiency of the screened hub genes to predict IDD. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to analyze function enrichment and signaling pathways. Protein-protein interaction (PPI) network was used to prioritize disease-related proteins. SERPINA1, ORM2, FGG and COL1A1 were identified through PRO-seq as the hub proteins involved in regulating IDD. ML algorithms selected ten hub genes, including IBSP, COL6A2, MMP2, SERPINA1, ACAN, FBLN7, LAMB2, TTLL7, COL9A3, and THBS4 in bRNA-seq. Since serine protease inhibitor clade A member 1 (SERPINA1) was the only common gene, its accuracy in degenerated and non-degenerated NP cells was validated using scRNA-seq. Then, the rat degeneration model of caudal vertebra was established. The expression of SERPINA1 and ORM2 was detected using immunohistochemical staining of human and rat intervertebral discs. The results showed that SERPINA1 was poorly expressed in the degenerative group. We further explored the potential function of SERPINA1 by Gene Set Enrichment Analysis (GSEA) and cell-cell communication. Therefore, SERPINA1 can be used as a biomarker to regulate or predict the progress of disc degeneration.
Toad venom-derived bufadienolides and their therapeutic application in prostate cancers: Current status and future directions
Authors: Ye Qingmei , Zhou Xin , Han Fangxuan , Zheng Caijuan
Published: March 2023

Cancer is the second leading cause of death worldwide. Specially, the high incidence rate and prevalence of drug resistance have rendered prostate cancer (PCa) a great threat to men’s health. Novel modalities with different structures or mechanisms are in urgent need to overcome these two challenges. Traditional Chinese medicine toad venom-derived agents (TVAs) have shown to possess versatile bioactivities in treating certain diseases including PCa. In this work, we attempted to have an overview of bufadienolides, the major bioactive components in TVAs, in the treatment of PCa in the past decade, including their derivatives developed by medicinal chemists to antagonize certain drawbacks of bufadienolides such as innate toxic effect to normal cells. Generally, bufadienolides can effectively induce apoptosis and suppress PCa cells in-vitro and in-vivo, majorly mediated by regulating certain microRNAs/long non-coding RNAs, or by modulating key pro-survival and pro-metastasis players in PCa. Importantly, critical obstacles and challenges using TVAs will be discussed and possible solutions and future perspectives will also be presented in this review. Further in-depth studies are clearly needed to decipher the mechanisms, e.g., targets and pathways, toxic effects and fully reveal their application. The information collected in this work may help evoke more effects in developing bufadienolides as therapeutic agents in PCa.
Transition-metal-catalyzed synthesis of quinazolines: A review
Authors: Tamatam Rekha , Kim Seok-Ho , Shin Dongyun
Published: March 2023

Quinazolines are a class of nitrogen-containing heterocyclic compounds with broad-spectrum of pharmacological activities. Transition-metal-catalyzed reactions have emerged as reliable and indispensable tools for the synthesis of pharmaceuticals. These reactions provide new entries into pharmaceutical ingredients of continuously increasing complexity, and catalysis with these metals has streamlined the synthesis of several marketed drugs. The last few decades have witnessed a tremendous outburst of transition-metal-catalyzed reactions for the construction of quinazoline scaffolds. In this review, the progress achieved in the synthesis of quinazolines under transition metal-catalyzed conditions are summarized and reports from 2010 to date are covered. This is presented along with the mechanistic insights of each representative methodology. The advantages, limitations, and future perspectives of synthesis of quinazolines through such reactions are also discussed.
Studying the effects of booster shots and antibody responses to the SARS-CoV-2 vaccination over time in health personnel
Authors: Wu Jingjing , Mu Hanyou , Pan Xiaowan , Guo Wenzheng
Published: March 2023

Background

With the emergence of mutant versions that lead to continual spreading and recurrent infections of SARS-CoV-2, the COVID-19 vaccines can assist protection for high risk groups, particularly health workers. Even while booster shots have been widely used, longitude studies on immune responses in healthy subjects are uncommon.

Methods

Eighty-five healthcare workers who received the BBIBP-CorV vaccine were prospectively enrolled and monitored for up to ten months. Automated Pylon immunoassays were used to quantify total anti-SARS-CoV2 antibody levels (TAb), surrogate neutralization antibody levels (NAb), and antibody avidities over the course of the follow-up. Additionally, hematology analyses were performed.

Results

Pylon antibody testing revealed that every participant tested negative at the beginning, and 88.2% of them tested positive about 14 days after receiving their second dosage. The TAb levels and NAb levels peaked in 76.5% and 88.2% of the subjects, respectively, at the same time. Age was connected with the peak antibody levels, but not with gender, BMI, or baseline hematological factors. The positive rates and the antibody levels had already started to decline three months following the second injection. The antibody levels and avidities quickly increased following the booster doses to levels that were considerably greater than the peak antibody responses before to the booster shots. Hematology testing revealed no safety concerns with immunizations.

Conclusion

In healthy workers, the two doses of BBIBP-CorV were able to induce humoral immunity; however, 3 months following vaccination, the antibody levels started to decline. The BBIBP-CorV booster injections increase both the quantity and quality of antibodies, which gave support for utilizing booster doses to prolong the duration of the vaccine’s protective effects.
Horizontal seismic wave at ground surface from transfer function based on ambient noise
Authors: Lim Doyoon , Ahn Jae-Kwang
Published: March 2023

Earthquake detection can be improved by ensuring that seismometer sites experience little artificial noise in the surrounding environment. To minimize noise, seismological stations should be positioned in rocky mountainous areas without nearby valleys, away from significant human activity. However, such surface sites may be scarce when constructing dense monitoring networks, necessitating the use of underground sites to ensure low noise levels. The Korean Meteorological Administration is currently installing new underground seismometers to increase seismic monitoring capacity. However, seismic data on the ground surface are also required for engineering technological developments (to reduce damage to structural components). Therefore, borehole seismic stations without surface seismometers need to estimate ground surface motion from borehole record data. We propose a transfer function that converts motion within boreholes to surface seismic waves using ambient noise, thereby facilitating estimation of ground surface motions using borehole seismometers. As a result, predicting ground surface motion from borehole record data becomes possible.