bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydrothermal Carbonization of Sewage Sludge: New Improvements in Phosphatic Fertilizer Production and Process Water Treatment Using Freeze Concentration
Authors: Gerner Gabriel , Chung Jae Wook , Meyer Luca , Wanner Rahel , Heiniger Simon , Seiler Daniel , Krebs Rolf , Treichler Alexander , Kontic Roman , Kulli Beatrice
Published: October 2023

In recent years, promising developments in the hydrothermal carbonization (HTC) of sewage sludge, as well as the potential to reclaim phosphorus and nitrogen, have emerged. In this study, the HTC of digested sewage sludge (DSS) was investigated for the downstream production of heavy metal (HM)-free fertilizer and the use of freeze concentration (FC) as a novel technology for process water treatment. To obtain clean fertilizer, phosphatic acid extracts were first treated with ion-exchange resins to remove dissolved HM, as well as phosphorus precipitating agents (i.e., aluminum and iron). Over 98% of the aluminum (Al) and 97% of the iron (Fe) could be removed in a single treatment step. The purified extract was then used for the precipitation of HM-free struvite crystals, with P-recovery rates exceeding 89%. Process water (PW) makes up the largest share of the two main HTC-products (i.e., hydrochar and PW) and is very rich in organic compounds. Compared to evaporation or membrane separation, FC is a promising technology for concentrating solutes from PW. Separation experiments resulted in the recovery of over 90% of the dissolved compounds in the concentrate. In our study, the concentrate was later utilized as an ammonium source for struvite precipitation, and the subsequent aerobic digestion of the remaining ice water resulted in an 85% reduction in chemical oxygen demand (COD) in 15 days.
Analysis of the Impact of Photovoltaic Curtain Walls Replacing Glass Curtain Walls on the Whole Life Cycle Carbon Emission of Public Buildings Based on BIM Modeling Study
Authors: Liu Meijing , Liu Changqi , Xie Hao , Zhao Zhonghui , Zhu Chong , Lu Yangang , Bu Changsheng
Published: October 2023

The construction industry plays a crucial role in achieving global carbon neutrality. The purpose of this study is to explore the application of photovoltaic curtain walls in building models and analyze their impact on carbon emissions in order to find the best adaptation method that combines economy and carbon reduction. Through a carbon emissions calculation and economic analysis of replacing photovoltaic curtain walls on a large public building in Zhenjiang, China, the results showed that after replacing glass curtain walls with photovoltaic curtain walls, the carbon emissions during the construction operation stage decreased by 30.74%, but the carbon emissions during the production and transportation stage of building materials increased by 10.48%. The carbon emissions throughout the entire life cycle of the building have been reduced by 20.99%. This indicates that photovoltaic curtain wall technology has the potential to reduce building carbon emissions. Further promoting the development of production technology and sales routes for photovoltaic curtain walls and accelerating the improvement of carbon trading systems can further improve the carbon emission reduction effect of buildings. This study provides practical reference for public buildings in similar areas and guidance for reducing carbon emissions in the future.
Subdiffusion–Superdiffusion Random-Field Transition
Authors: Iomin Alexander
Published: October 2023

A contaminant spreading affected by a random field at boundaries in the comb geometry is considered. The physical effect of the random boundary conditions results in increasing a transport exponent such that the mean squared displacement increases with time from t12 to t12+5α/2 for real 0≤α≤1. This stochastic acceleration due to these space-time-dependent boundary conditions leads to a transition from subdiffusion to superdiffusion. Experimentally, it can be realized by controlling the boundary conditions of 2D diffusion in the comb geometry.
Effects of Artificial Intelligence on Surgical Patients’ Health Education
Authors: Huang Hsin-Shu , Fang Hsin-Yuan
Published: October 2023

Today, the various abilities that nurses require to meet patients’ healthcare needs adequately are all affected by AI-enabled systems. This research used an experimental study design in which 60 subjects were randomly assigned to either an experimental (AI image e-book guidance) group or a control (text paper guidance) group after meeting the admission conditions and agreeing to participate in the study. It was proven that providing AI image e-book guidance before surgery significantly changed the behavior of patients and promoted relief of urinary catheter discomfort through self-efficacy to reduce urinary catheter pain after surgery (p < 0.001). It was found that providing AI image e-book guidance can shorten the time for health education and provide patients with repeated medical education and familiarity with health guidance, which can help to address the important clinical service demand issue and the shortage of nursing staff.
QoL, CIs, QALYs, and Individualized Rehabilitation: The Clinical and Practical Benefits of Regularly Assessing the Quality of Life of Adult Cochlear Implant Recipients
Authors: Lassaletta Luis , Calvino Miryam , Sanchez-Cuadrado Isabel , Skarzynski Piotr Henryk , Cywka Katarzyna B. , Czajka Natalia , Kutyba Justyna , Tavora-Vieira Dayse , Van de Heyning Paul , Mertens Griet , Staecker Hinrich , Humphrey Bryan , Zernotti Mario , Zernotti Maximo , Magele Astrid , Ploder Marlene , Zabeu Julia Speranza
Published: October 2023

This study aimed to report quality of life (QoL) scores in unilateral cochlear implant (CI) users and to generate guidance for clinicians on using QoL measures to individualize CI counselling and rehabilitation and to increase access to CIs as a mode of rehabilitation. Participants (n = 101) were unilateral CI users with single-sided deafness (SSD; n = 17), asymmetrical hearing loss (AHL; n = 26), or bilateral hearing loss (Uni; n = 58). Generic QoL was assessed via the Health Utilities Index (HUI-3), and disease-specific QoL was assessed via the Speech, Spatial, and Qualities of Hearing scale (SSQ12) and Nijmegen CI Questionnaire (NCIQ) at preimplantation and at 6 and 12 months of CI use. All groups had significantly increased HUI-3 scores at both intervals. The SSD group showed significant benefit on the SSQ12 at visit 3, the AHL group showed significant benefit on the SSQ12 and most NCIQ subdomains at both intervals, and the Uni group showed significant benefit with both tests at both intervals. Unilateral CI recipients demonstrate improved QoL within the first 12 months of device use. Regular assessment with generic and disease-specific questionnaires has the potential to play an important role in personalizing treatment and possibly in increasing access to CI provision.
Moving beyond Inclusion to Belonging
Authors: Long Toby , Guo Jennifer
Published: October 2023

This paper explores the concepts of inclusion, participation, and belonging in the context of development for children with disabilities. The importance of creating an environment that embraces diversity, encourages active engagement, and nurtures a sense of belonging for children is discussed. The authors provide insights into the benefits of inclusive practices, strategies to enhance participation, and methods to foster a sense of belonging in children with disabilities. The authors argue that service providers and service systems must move beyond fostering social inclusion and inclusive education, although emphasized globally, and focus on promoting participation and ultimately belonging to ensure that children with disabilities are full members of their communities.
The Spatiotemporal Pattern Evolution and Driving Force of Tourism Information Flow in the Chengdu–Chongqing City Cluster
Authors: Zhao Yang , Wang Zegen , Yong Zhiwei , Xu Peng , Wang Qian , Du Xuemei
Published: October 2023

In recent years, the tourism industry has developed rapidly. However, traditional tourism information has the disadvantages of slow response speed and limited information content, which cannot reflect the evolution trend of spatial and temporal patterns of tourism information in time. Here, based on the Baidu Index, we construct an evaluation framework to analyse the spatial and temporal flow of tourism information in the Chengdu–Chongqing urban cluster from 2011 to 2021. Then, we analyse the urban links between different network levels from the evolution pattern. Finally, we use the geodetector model to analyse its driving mechanism. The results show that Chengdu and Chongqing are the most active cities in the study area in terms of tourism information. The unbalanced development of tourism information between Chengdu and Chongqing and other cities in the region gradually deepens during the period 2011–2019 (polarization effect), but the unbalanced development moderates after 2019. On the other hand, cities in the middle of the Chengdu–Chongqing cluster always have weak agglomeration effects of tourism information. Cities with high tourism information outflow rates in the Chengdu–Chongqing city cluster are mainly concentrated around Chengdu. The average outflow rate of Deyang is the highest, at 27.8%. Cities with low tourist information outflow rates are primarily located in the west, central and south. Ya’an is the city with the lowest outflow rate, with an average of −62.2%. Specifically, Chengdu is the dominant and most radiantly influential city. The tourism information of the Chengdu–Chongqing urban cluster shows a radial network with Chengdu and Chongqing as the core. The driving force analysis shows that the push factor of tourist source, such as the number of people buying pension insurance, is the core driving mechanism, while the pull factor of destination, such as the park green area, and resistance factors such as psychological distance, are in the secondary position. In general, this paper uses Internet tourism data to expand the traditional tourism information research of the Chengdu–Chongqing urban cluster, which can better respond to the changes and needs of the tourism market and provide reference for the spatial optimization of tourism destinations.
The Clinical and Molecular Profile of Lung Cancer Patients Harboring the TP53 R337H Germline Variant in a Brazilian Cancer Center: The Possible Mechanism of Carcinogenesis
Authors: Lopes Carlos D. H. , Antonacio Fernanda F. , Moraes Priscila M. G. , Asprino Paula F. , Galante Pedro A. F. , Jardim Denis L. , de Macedo Mariana P. , Sandoval Renata L. , Katz Artur , de Castro Gilberto , Achatz Maria Isabel
Published: October 2023

In southern and southeastern Brazil, the TP53 founder variant c.1010G>A (R337H) has been previously documented with a prevalence of 0.3% within the general population and linked to a heightened incidence of lung adenocarcinomas (LUADs). In the present investigation, we cover clinical and molecular characterizations of lung cancer patients from the Brazilian Li-Fraumeni Syndrome Study (BLISS) database. Among the 175 diagnosed malignant neoplasms, 28 (16%) were classified as LUADs, predominantly occurring in females (68%), aged above 50 years, and never-smokers (78.6%). Significantly, LUADs manifested as the initial clinical presentation of Li-Fraumeni Syndrome in 78.6% of cases. Molecular profiling was available for 20 patients, with 14 (70%) revealing EGFR family alterations. In total, 23 alterations in cancer driver genes were identified, comprising 7 actionable mutations and 4 linked to resistance against systemic treatments. In conclusion, the carriers of TP53 R337H demonstrate a predisposition to LUAD development. Furthermore, our results indicate that environmental pollution potentially impacts the carcinogenesis of lung tumors in the carriers of TP53 R337H.
Structural and Genetic Identification of the O-Antigen from an Escherichia coli Isolate, SD2019180, Representing a Novel Serogroup
Authors: Wang Jing , Qin Chunjun , Xu Yujuan , Yin Jian , Hu Jing , Guo Xi
Published: October 2023

The O-antigen is one of the outermost surface components of Gram-negative bacteria. Its large structural variation provides the molecular basis for bacterial serological diversity. Here, we established the structure of the O-antigen from an Escherichia coli strain, SD2019180, which appeared to be completely different from the known E. coli serogroups. The O-antigen tetrasaccharide biological repeating unit was identified as → 2)-[β-d-GlcpA-(1 → 4)]-[α-d-Galp-(1 → 3)]-α-l-Fucp-(1 → 3)-α-d-GlcpNAc-(1 →. Furthermore, we analyzed the O-antigen gene cluster of SD2019180 and confirmed its role in O-antigen synthesis by using deletion and complementation experiments. Our findings indicate that SD2019180 is a novel serogroup of Escherichia coli.
Experimental Validation of the Numerical Model for Oil–Gas Separation
Authors: Tomescu Sorin Gabriel , Mălăel Ion , Conțiu Rareș , Voicu Sebastian
Published: October 2023

The oil and gas sector is important to the global economy because it covers the exploration, production, processing, transportation, and distribution of oil and natural gas resources. Despite constant innovation and development of technologies to improve efficiency, reduce environmental impact, and optimize operations in the gas and oil industry over the last few decades, there is still room to increase the efficiency of the industry’s equipment in order to reduce its carbon footprint. The separation of gas from oil is a critical stage in the technological production chain, and it is carried out using high-performance multi-phase separators to limit greenhouse gas emissions and have a low impact on the environment. In this study, an improved gas–oil separator configuration was established utilizing CFD techniques. Two separator geometry characteristics were studied. Both cases have the same number of subdomains, two porous media, and four fluid zones, but with a difference in the pitch of the cyclone from the inlet subdomain. The streamlines in a cross-plan of the separator and the distribution of the oil volume fraction from the intake to the outlet were two of the numerical results that were shown as numeric outcomes. The validation of these results was performed using an experimental testing campaign that had the purpose of determining the amount of lubricating oil that is discharged together with the compressed gas at the separator outlet.
New Eco-Friendly, Biocompatible, Bactericidal, Fungicidal and Anticancer-Activity-Exhibiting Nanocomposites Based on Bimetallic TiO2@Cr2O3 Nanoparticle Core and Biopolymer Shells
Authors: Hasanin Mohamed S. , Elhenawy Yasser , Abdel-Hamid Shereen M. S. , Fouad Yasser , Monica Toderaș , Al-Qabandi O. A. , El Fray Miroslawa , Bassyouni Mohamed
Published: October 2023

Nanoparticles have attracted substantial attention for their diverse range of applications, particularly in biomedicine applications and drug delivery, owing to their unique properties. However, their tiny size facilitates easy cellular entry, which can also lead to interactions with cellular components, potentially resulting in toxicity and undesirable effects. In this study, a novel nanocomposite formulation was developed using biopolymers, specifically ethylcellulose and collagen, as capping and stabilizing agents to create bimetallic nanoparticles including TiO2@Cr2O3 nanoparticles. Physicochemical and morphological analyses were carried out to validate the formulation’s structure. The obtained characteristics emphasized the presence of a nanostructure involving bimetallic nanoparticles. This formulation exhibited excellent biological activity, including high biocompatibility with Vero and WI38 cells at concentrations of 40.4 and 52 µg/mL, respectively, as well as effective anticancer activity with significant selectivity. The IC50 values were determined to be 19 and 22 µg/mL for MCF7 and A549 cells, respectively. The antimicrobial assessment revealed the highest MIC value for A. niger at 50 µg/mL, while the lowest MIC value was observed for Gram-positive bacteria at 3.12 µg/mL. Additionally, the nanocomposite demonstrated antioxidant activity at a low concentration of 1.5 µg/mL.
Optimization of Agricultural Resource Allocation among Crops: A Portfolio Model Analysis
Authors: Miao Bao-Li , Liu Ying , Fan Yu-Bing , Niu Xue-Jiao , Jiang Xiu-Yun , Tang Zeng
Published: October 2023

The portfolio model provides a new framework that enables farmers to make tradeoffs between the returns and risks of agricultural production. However, it has only been used in cost allocation among resources and in farmland allocation among crops at present. Thus, we conducted a portfolio model analysis based on an independent, mixed cross-section of data accounting for 1650 rural households in Minqin county, China, over a timescale of three years; the goal was to explore the feasibility and actuating extent of the portfolio model in optimizing the allocation of agricultural resources among crops other than farmland. The results showed that, by optimizing resource allocation among crops using a portfolio model, the return per unit resource of farmland, water, and labor increased by CNY 4.03 thousand per hectare, CNY 0.38 per cubic meter, and CNY 39.75 per day, without increasing risk, and with corresponding increases in use efficiency of 14.64%, 9.25%, and 13.55%, respectively, in the study area. Therefore, the portfolio model is a good tool for agricultural resource allocation optimization. Policy makers can manage agricultural resources according to their risk preference using this model. This study provides new insights into agricultural resource allocation and critical empirical evidence for return and risk management, as well as resource use efficiency improvement in agricultural production.
Spatiotemporal Characteristics and Determinants of Rural Construction Land in China’s Developed Areas: A Case Study of the Yangtze River Delta
Authors: Niu Fangqu , Wang Lan , Sun Wei
Published: October 2023

Rural construction land (RCL) received less attention but played an important role to control rural land use. Studying the RCL of developed areas may provide valuable references for underdeveloped areas to optimize land use. The Yangtze River Delta (YRD) is the most economically developed region in China. The study is intended to explore the spatiotemporal characteristics and determinants of RCL in the YRD based on a period of data from 1990 to 2017. The results show that the RCL in the YRD increases at an average annual rate of 5.38% but the growth rate tends to decrease. There is a weak spatial linkage of the RCL growth between cities. Clear spatial differences exist in the effects of every determinant of RCL. The correlation between the rural population and the RCL is unstable, which proves the existence of hollow villages. There is no clear correlation between the RCL and the local economy and accessibility, as the rural population normally goes to few big cities for higher salary work but spends the money in their hometowns on building homes. These findings help optimize rural land use in the YRD and provide an important reference for planning land use in underdeveloped regions.
HPMCAS-Based Amorphous Solid Dispersions in Clinic: A Review on Manufacturing Techniques (Hot Melt Extrusion and Spray Drying), Marketed Products and Patents
Authors: Corrie Leander , Ajjarapu Srinivas , Banda Srikanth , Parvathaneni Madhukiran , Bolla Pradeep Kumar , Kommineni Nagavendra
Published: October 2023

Today, therapeutic candidates with low solubility have become increasingly common in pharmaceutical research pipelines. Several techniques such as hot melt extrusion, spray drying, supercritical fluid technology, electrospinning, KinetiSol, etc., have been devised to improve either or both the solubility and dissolution to enhance the bioavailability of these active substances belonging to BCS Class II and IV. The principle involved in all these preparation techniques is similar, where the crystal lattice of the drug is disrupted by either the application of heat or dissolving it in a solvent and the movement of the fine drug particles is arrested with the help of a polymer by either cooling or drying to remove the solvent. The dispersed drug particles in the polymer matrix have higher entropy and enthalpy and, thereby, higher free energy in comparison to the crystalline drug. Povidone, polymethaacrylate derivatives, hydroxypropyl methyl cellulose (HPMC) and hydroxypropyl methylcellulose acetate succinate derivatives are commonly used as polymers in the preparation of ASDs. Specifically, hydroxypropylmethylcellulose acetate succinate (HPMCAS)-based ASDs have become well established in commercially available products and are widely explored to improve the solubility of poorly soluble drugs. This article provides an analysis of two widely used manufacturing techniques for HPMCAS ASDs, namely, hot melt extrusion and spray drying. Additionally, details of HPMCAS-based ASD marketed products and patents have been discussed to emphasize the commercial aspect.
Development of Polyhydroxybutyrate-Based Packaging Films and Methods to Their Ultrasonic Welding
Authors: Talaniuk Viktoriia , Godzierz Marcin , Vashchuk Alina , Iurhenko Maksym , Chaber Paweł , Sikorska Wanda , Kobyliukh Anastasiia , Demchenko Valeriy , Rogalsky Sergiy , Szeluga Urszula , Adamus Grażyna
Published: October 2023

This study developed a technical task associated with the formation of welded joints based on biodegradable polymers and their subsequent physicochemical characterization. The primary objective was to establish the effect of the welding process and modification of natural poly(3-hydroxybutyrate) (PHB) with N,N-dibutylundecenoylamide (DBUA) as a plasticizing agent on the structure and properties of PHB-based biopolymer materials as well as the process and structure of welded joints formation using ultrasonic welding technique. The weldability of biodegradable layers based on PHB and PHB/DBUA mixture was ultrasonically welded and optimized using a standard Branson press-type installation. The effect of the DBUA plasticizer and welding process on the structure of PHB-based biodegradable material was investigated using scanning electron microscopy, X-ray diffraction, FT-IR spectroscopy, differential scanning calorimetry, and thermomechanical analysis. The results confirmed that the DBUA acted as an effective plasticizer of PHB, contributing to lower crystallinity of the PHB/DBUA mixture (63%) in relation to the crystallinity degree of pure PHB film (69%). Ultrasonic welding resulted in an additional increase (approximately 8.5%) in the degree of crystallinity in the PHB/DBUA in relation to the initial PHB/DBUA mixture. The significant shift toward lower temperatures of the crystallization and melting peaks of PHB modified with DBUA were observed using DSC concerning pure PHB. The melt crystallization process of PHB was affected by welding treatment, and a shift toward higher temperature was observed compared with the unwelded PHB/DBUA sample. The butt-welded joints of biodegradable PHB/DBUA materials made using the ultrasonic method tested for tensile strength have damaged the area immediately outside the joining surface.
Numerical Simulations of Carbon-Fibre Impregnation with a Polymer as an Anisotropic Permeability Medium
Authors: Gomes Daniel , Amorim Luís , Santos Raquel M. , Gonçalves Nelson D.
Published: October 2023

The impregnation process of carbon fibres with polymers is challenging to model due to the system’s complexity, particularly concerning the following aspects: the complex rheology of the polymeric matrices and the presence of solid, continuous fibres, both with anisotropic properties, and the interaction between solid and fluid, which can change the displacement of fibres into a cyclic dependence. In this work, an interesting approach was considered by setting the fibres as a porous medium whose properties were calculated with microscale/macroscale cycle modelling. In the microscale modelling stage, two main assumptions can be made: (i) a homogeneous distribution with a representative cell or (ii) a stochastic distribution of fibres. The solution to the abovementioned flow and fibre distribution problem can severely differ with only a slight change in a single parameter for a given set of processing parameters. Therefore, the influence of some of them during the fibre impregnation process was evaluated, allowing a shortcut for the polymer through a gap between fibres and the bottom wall of the extrusion die. The range of investigated values regarding the gap enables one to cover good impregnation conditions up to the occurrence of the shortcut and consequent poor impregnation quality. These studies were performed with numerical simulations with circa 126,000 degrees of freedom, considering the discretisation mesh elements and the unknowns (pressure and two velocity components).
Prognostic Value of Mitral Regurgitation in Patients with Primary Hypertrophic Cardiomyopathy
Authors: Tesic Milorad , Travica Lazar , Giga Vojislav , Jovanovic Ivana , Trifunovic Zamaklar Danijela , Popovic Dejana , Mladenovic Djordje , Radomirovic Marija , Vratonjic Jelena , Boskovic Nikola , Dedic Srdjan , Nedeljkovic Arsenovic Olga , Aleksandric Srdjan , Juricic Stefan , Beleslin Branko , Djordjevic Dikic Ana
Published: October 2023

Background and Objectives: Mitral valve pathology and mitral regurgitation (MR) are very common in patients with hypertrophic cardiomyopathy (HCM), and the evaluation of mitral valve anatomy and degree of MR is important in patients with HCM. The aim of our study was to examine the potential influence of moderate or moderately severe MR on the prognosis, clinical presentation, and structural characteristics of HCM patients. Materials and Methods: A prospective study examined 176 patients diagnosed with primary asymmetric HCM. According to the severity of the MR, the patients were divided into two groups: Group 1 (n = 116) with no/trace or mild MR and Group 2 (n = 60) with moderate or moderately severe MR. All patients had clinical and echocardiographic examinations, as well as a 24 h Holter ECG. Results: Group 2 had significantly more often the presence of the obstructive type of HCM (p < 0.001), syncope (p = 0.030), NYHA II class (p < 0.001), and atrial fibrillation (p = 0.023). Also, Group 2 had an enlarged left atrial dimension (p < 0.001), left atrial volume index (p < 0.001), and indirectly measured systolic pressure in the right ventricle (p < 0.001). Patients with a higher grade of MR had a significantly higher E/e′ (p < 0.001) and, as a result, higher values of Nt pro BNP values (p < 0.001) compared to Group 1. Kaplan–Meier analysis demonstrated that the event-free survival rate during a median follow-up of 88 (IQR 40–112) months was significantly higher in Group 1 compared to Group 2 (84% vs. 45% at 8 years; log-rank 20.4, p < 0.001). After adjustment for relevant confounders, the presence of moderate or moderately severe MR remained as an independent predictor of adverse outcomes (HR 2.788; 95% CI 1.221–6.364, p = 0.015). Conclusions: The presence of moderate or moderately severe MR was associated with unfavorable long-term outcomes in HCM patients.
The Prediction of IVF Outcomes with Autologous Oocytes and the Optimal MII Oocyte/Embryo Number for Live Birth at Advanced Maternal Age
Authors: Havrljenko Jelena , Kopitovic Vesna , Pjevic Aleksandra Trninic , Milatovic Stevan , Pavlica Tatjana , Andric Nebojsa , Pogrmic-Majkic Kristina
Published: October 2023

Background and Objectives: Delayed childbearing in advanced age might be associated with a low prognosis for achieving pregnancy. Therefore, it is important to establish a predictive tool that will optimize the likelihood of a live birth at advanced age. Material and Methods: The retrospective study was conducted at the Ferona Fertility Clinic in Novi Sad (Republic of Serbia), between January 2020 and May 2021. The survey included 491 women aged ≥35 who met the inclusion criteria and who were subjected to an IVF (in vitro fertilization) treatment cycle. Results: The average number of retrieved oocytes, MII (metaphase II) oocytes, and developed embryos significantly decreased in advanced age. Age was also found to have a significant adverse effect on pregnancy and live birth rates. In women aged ≥35, 10/12 MII oocytes or 10/11 embryos are required for reaching an optimal live birth rate/cumulative live birth rate. Optimal CLBR (cumulative live birth rate) per one oocyte was achieved when 9 MII oocyte were retrieved. Conclusions: The study indicates that the cut-off for increased risk is ≥42 year. However, despite low live birth rates, autologous IVF for these women is not futile. An increase in the number of retrieved mature oocytes and a generation of surplus cryopreserved embryos could reinforce LBR (live birth rate) and CLBR. Clinicians should be very cautious in counseling, as autologous IVF may only be applicable to women with good ovarian reserve.
Endometrial Osseous Metaplasia—A Rare Cause of Infertility with Unknown Etiology
Authors: Tica Vlad Iustin , Postolache Iulia , Boșoteanu Madalina , Aschie Mariana , Tica Irina , Orasanu Cristian Ionut , Penciu Roxana Cleopatra , Tica Andrei Adrian , Steriu Liliana , De Wilde Rudy Leon , Tica Oana Sorina
Published: October 2023

Background: Osseous tissue in the endometrium is a rare find, and it is most often discovered when the patient presents with infertility. It is frequently associated with dysmenorrhea and abnormal menstrual bleedings. Although its etiology remains unclear, in almost all described cases until now, the patient has an obstetrical history. Case report: In this report, we present a unique case of endometrial osseous metaplasia in a 27-year-old primary infertile patient. The transvaginal ultrasound revealed a 18/13/7 mm hyperechoic endometrial mass with posterior acoustic shadowing and no flow on color Doppler. A hysteroscopic examination found a polygonal calcification on the endometrial posterior face of the uterine cavity, in the corporeal isthmic region, which was extracted. The histopathological evaluation revealed microscopic elements compatible with endometrial calcification. The patient had a good postoperative course and the complex endocrinologic, immunologic and electrolytical investigation failed to prove any abnormality. Follow-up transvaginal ultrasound examinations revealed no modifications. Three years later, the patient conceived spontaneously, had an uneventful pregnancy and delivered a full-term fetus. Conclusion: We assumed that this entity can be a serious cause of infertility since the patient had a long history of (primary) infertility and its resection made the pregnancy’s occurrence possible. Finally, since neither history of abortion or chronic inflammation nor any abnormal laboratory test were noticed, we concluded that the etiology of this entity remained unclear.
Comparative Efficacy of Traditional Corticotomy and Flapless Piezotomy in Facilitating Orthodontic Tooth Movement: A Systematic Review and Meta-Analysis
Authors: Han Sung-Hoon , Park Won-Jong , Park Jun-Beom
Published: October 2023

Background and Objectives: This study aimed to perform a meta-analysis comparing the effects of corticotomy and flapless piezocision on accelerated tooth movement. Materials and Methods: A comprehensive search using a combination of controlled vocabulary (MeSH) and free-text terms was undertaken by two reviewers to identify published systematic reviews. Three major electronic databases (Medline via PubMed, Cochrane Database, and Embase) were searched up to 2 June 2023. Results: The results of the meta-analysis showed that the pooled standardized mean difference values of accumulative movement distances for flapless piezocision were 1.43 (95% CI, 0.38 to 2.48; p < 0.01), 1.09 (95% CI, −0.08 to 2.26; p = 0.07), and 0.73 (95% CI, −0.58 to 4.02; p = 0.14). The results of the meta-analysis demonstrated that the pooled SMD values of accumulative movement distances for the corticotomy were 2.76 (95% CI, 0.18 to 5.34; p = 0.04), 1.43 (95% CI, −1.10 to 3.96; p = 0.27), and 4.78 (95% CI, −4.54 to 14.10; p = 0.32). Although the test for overall effectiveness was significant for piezocision and corticotomy, there were no significant differences between piezocision and corticotomy. Conclusions: The study determined that both conventional corticotomy and flapless piezosurgery are effective as adjuncts to orthodontic treatment. Moreover, no significant difference was observed in the short-term effectiveness of canine retraction acceleration between conventional corticotomy and flapless piezocision. While piezocision may be a favorable option for orthodontic treatment, corticotomy can be considered in cases requiring additional procedures such as bone grafting.
Efficient Adsorption and Electrochemical Detection of Cd2+ with a Ternary MgZnFe-Layered Double Hydroxides Engineered Porous Biochar Composite
Authors: Yu Yongfang , Yang Wenting , Li Shujuan , Gao Yansha , Wang Linyu , Huang Guoqin
Published: October 2023

Their unique layered structure, large specific surface area, good stability, high negative charge density between layers, and customizable composition give layered double hydroxides (LDHs) excellent adsorption and detection performance for heavy metal ions (HMIs). However, their easy aggregation and low electrical conductivity limit the practical application of untreated LDHs. In this work, a ternary MgZnFe-LDHs engineered porous biochar (MgZnFe-LDHs/PBC) heterojunction was proposed as a sensing and adsorption material for the effective detection and removal of Cd2+ from wastewater. The growth of MgZnFe-LDHs in the PBC pores not only reduces the accumulation of MgZnFe-LDHs, but also improves the electrical conductivity of the composite. The synergistic effect between MgZnFe-LDHs and PBC enables the composite to achieve a maximum adsorption capacity of up to 293.4 mg/g for Cd2+ in wastewater. Meanwhile, the MgZnFe-LDHs/PBC-based electrochemical sensor shows excellent detection performance for Cd2+, presenting a wide linear range (0.01 ng/L–1 mg/L), low detection limit (3.0 pg/L), good selectivity, and stability. The results indicate that MgZnFe-LDHs/PBC would be a potential material for detecting and removing Cd2+ from wastewater.
Dark-Chocolate-Coated BRS Clara Raisins: Phenolic Composition and Sensory Attributes
Authors: Olivati Carolina , Nishiyama-Hortense Yara Paula , Soares Janzantti Natália , da Silva Roberto , Lago Vanzela Ellen Silva , Gómez-Alonso Sergio
Published: October 2023

Dark chocolate dragée confectionary was made with BRS Clara raisins pre-treated with extra virgin olive oil (EVOO). The evaluation of the changes in the phenolic composition (flavonols, hydrocinnamic acid derivatives (HCADs), stilbenes and flavan-3-ol monomers, dimers, and proanthocyanidins (PAs)) resulting from the covering process showed that the chocolate coating was responsible for an increase in the concentrations of flavan-3-ols and PAs when compared to just the raisins. For the flavonols and HCADs, a reduction in the total concentration of compounds was observed when comparing the dragées to the raisins. Furthermore, there was a strong influence of chocolate in the qualitative profile with the emergence of new compounds (quercetin-3-pentoside, kampfterol-3-rutinoside, p-coumaric acid, and caffeoyl-aspartate). The combination of these ingredients (raisins and chocolate) resulted in a dark chocolate coated raisin (DC) with good sensory acceptance and a more complex phenolic composition that may positively contribute to its functional quality.
Determination of Luteolin 7-Glucuronide in Perilla frutescens (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect
Authors: Wu Zhaoyang , Lee Sangyoun , Kang Beomgoo , Lee Sookyeong , Koo Kyochul , Lee Jaeyong , Lim Soonsung
Published: October 2023

Lowering blood cholesterol levels is crucial for reducing the risk of cardiovascular disease in patients with familial hypercholesterolemia. To develop Perilla frutescens (L.) Britt. leaves as a functional food with a cholesterol-lowering effect, in this study, we collected P. frutescens (L.) Britt. leaves from different regions of China and Republic of Korea. On the basis of the extraction yield (all components; g/kg), we selected P. frutescens (L.) Britt. leaves from Hebei Province, China with an extract yield of 60.9 g/kg. After evaluating different concentrations of ethanol/water solvent for P. frutescens (L.) Britt. leaves, with luteolin 7-glucuronide as the indicator component, we selected a 30% ethanol/water solvent with a high luteolin 7-glucuronide content of 0.548 mg/g in Perilla. frutescens (L.) Britt. leaves. Subsequently, we evaluated the cholesterol-lowering effects of P. frutescens (L.) Britt. leaf extract and luteolin 7-glucuronide by detecting total cholesterol in HepG2 cells. The 30% ethanol extract lowered cholesterol levels significantly by downregulating 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase expression. This suggests that P. frutescens (L.) Britt leaves have significant health benefits and can be explored as a potentially promising food additive for the prevention of hypercholesterolemia-related diseases.
Tunable Supramolecular Ag+-Host Interactions in Pillar[n]arene[m]quinones and Ensuing Specific Binding to 1-Alkynes
Authors: Zhu Yumei , Escorihuela Jorge , Wang Haiying , Sue Andrew C.-H. , Zuilhof Han
Published: October 2023

We developed an improved, robust synthesis of a series of pillar[6]arenes with a varying number (0–3) of quinone moieties in the ring. This easy-to-control variation yielded a gradually less electron-rich cavity in going from zero to three quinone units, as shown from the strength of host–guest interactions with silver ions. Such macrocycle-Ag2 complexes themselves were shown to display an unprecedented, sharp distinction between terminal alkynes, which strongly bound to such complexes, and internal alkynes, internal alkenes and terminal alkenes, which do hardly bind.
Development and Comparative Evaluation of Two Highly Sensitive Immunosensor Platforms for Trace Determination of Copper Ions in Drinking Water Using a Monoclonal Antibody Specific to Copper-EDTA Complex
Authors: Darwish Ibrahim A. , Wang Zongzhi , Darling Ryhan J.
Published: October 2023

This study describes the development of two highly sensitive immunosensor platforms for the trace determination of copper ions, Cu(II), in drinking water. These platforms were a microwell-based enzyme-linked immunosorbent assay (ELISA) and a kinetic exclusion assay (KinExA) with a KinExATM 3200 immunosensor. Both ELISA and KinExA were developed utilizing the same antibody and coating reagent. The antibody was a mouse monoclonal antibody, designated as 8D66, that specifically recognized Cu(II)-ethylenediamine tetraacetic acid complex (Cu(II)-EDTA) but did not recognize Cu(II)-free EDTA. The 8D66 monoclonal antibody was generated by the fusion of spleen cells of an immunized BALB/c mouse with SP2/0-Ag14 myeloma cells. The immunogen was a protein conjugate of Cu(II)-EDTA with keyhole limpet hemocyanin protein. The coating reagent was Cu(II)-EDTA covalently linked to bovine serum albumin protein (Cu(II)-EDTA-BSA). Both assays involved the competitive binding reaction between Cu(II)-EDTA complexes, formed in the sample solution, and Cu(II)-EDTA-BSA conjugate which has been immobilized onto ELISA plates (in ELISA) or polymethylmethacrylate beads (in KinExA) for a limited quantity of binding sites of the 8D66 antibody. In ELISA, color signals were generated by a peroxidase-labeled secondary antibody and 3,3′,5,5′-tetramethylbenzidine substrate. In KinExA, a fluorescein isothiocyanate-labeled secondary antibody was used to generate KinExAgram (trend-line fluorescence responses vs. time). The conditions of both ELISA and KinExA were investigated, and the optimum procedures were established. Both ELISA and KinExA were validated, and all validation parameters were acceptable. Many different metal ions that are commonly encountered in drinking water did not interfere with the Cu(II) analysis by both ELISA and KinExA. Both assays were applied to the determination of Cu(II) in drinking water with satisfactory accuracy and precision. Both assays were compared favorably with inductively coupled plasma atomic emission spectroscopy in terms of their abilities to accurately and precisely determine Cu(II) in drinking water samples. A comparative evaluation of ELISA and KinExA revealed that KinExA had a higher sensitivity and better precision than ELISA, whereas both assays had comparable accuracy. Both ELISA and KinExA were superior to the existing atomic spectrometric methods for Cu(II) in terms of sensitivity, convenience, and analysis throughputs. The proposed ELISA and KinExA are anticipated to effectively contribute to assessing Cu(II) concentrations and control the exposure of humans to its potential toxicities.
The Content of Bioactive Compounds and Technological Properties of Matcha Green Tea and Its Application in the Design of Functional Beverages
Authors: Najman Katarzyna , Sadowska Anna , Wolińska Monika , Starczewska Katarzyna , Buczak Krzysztof
Published: October 2023

Matcha is a powdered green tea obtained from the Camellia sinensis L. plant intended for both “hot” and “cold” consumption. It is a rich source of bioactive ingredients, thanks to which it has strong antioxidant properties. In this research, an organoleptic evaluation was carried out, and the physical characteristics (i.e., instrumental color measurement (L*a*b*), water activity, water solubility index (WSI), water holding capacity (WHC) of 10 powdered Matcha green teas, and in the 2.5% Matcha water solutions, pH, °Brix and osmolality were tested. Also, the content of phenolic ingredients, i.e., selected phenolic acids, flavonoids and total polyphenols, was assessed. The content of chlorophyll, vitamin C and antioxidant potential were also examined. Matcha M-4 was used to design two functional model beverages, in the form of ready-to-use powdered drinks, consisting of Matcha green tea, protein preparations, inulin, maltodextrin and sugar. The obtained powdered drink, when dissolved in the preferred liquid (water, milk, juice), is regenerative, high-protein and rich in bioactive ingredients from the Matcha drink, with prebiotic properties derived from the added inulin. The beverage is also characterized by low osmolality. It can be recommended as a regenerating beverage for a wide group of consumers, athletes and people with deficiencies, among others protein, and elderly people, as well as in the prevention and supportive treatment of bone and joint tissue diseases.
L-Arginine Enhances Oral Keratinocyte Proliferation under High-Glucose Conditions via Upregulation of CYP1A1, SKP2, and SRSF5
Authors: Shi Junhe , Leonardo Trevor R. , Han Chen , Bangash Hiba I. , Chen Dandan , Trivedi Harsh M. , Chen Lin
Published: October 2023

High glucose inhibits oral keratinocyte proliferation. Diabetes can lead to delayed oral wound healing and periodontal disease. L-Arginine, one of the most versatile amino acids, plays an important role in wound healing, organ maturation, and development. In this study, L-Arginine was found to enhance oral keratinocyte proliferation under high-glucose conditions. RNA sequencing analysis discovered a significant number of genes differentially upregulated following L-Arginine treatment under high-glucose conditions. Cytochrome P450 family 1 subfamily A member 1 (CYP1A1) was the most significantly upregulated gene at 24 and 48 h after L-Arginine treatment. Gene Ontology enrichment analysis found that cell proliferation- and mitosis-related biological processes, such as mitotic nuclear division, mRNA processing, and positive regulation of cell cycle processes, were significantly upregulated. Pathway enrichment analysis found that S-phase kinase-associated protein 2 (SKP2) and serine- and arginine-rich splicing factor 5 (SRSF5) were the top upregulated genes in cell cycle and spliceosome pathways, respectively. Indirect immunofluorescent cytochemistry confirmed increased protein levels of CYP1A1, SKP2, and SRSF5 after L-Arginine treatment. Knockdown of CYP1A1, SKP2, and SRSF5 abolished the enhanced proliferative effect of L-Arginine on oral keratinocytes under high-glucose conditions. In conclusion, L-Arginine enhances oral keratinocyte proliferation under high-glucose conditions via upregulation of CYP1A1, SKP2, and SRSF5, suggesting that supplemental L-Arginine in oral care products may be beneficial for oral tissue repair and regeneration.
Optimized Dropkey-Based Grad-CAM: Toward Accurate Image Feature Localization
Authors: Liu Yiwei , Tang Luping , Liao Chen , Zhang Chun , Guo Yingqing , Xia Yixuan , Zhang Yangyang , Yao Sisi
Published: October 2023

Regarding the interpretable techniques in the field of image recognition, Grad-CAM is widely used for feature localization in images to reflect the logical decision-making information behind the neural network due to its high applicability. However, extensive experimentation on a customized dataset revealed that the deep convolutional neural network (CNN) model based on Gradient-weighted Class Activation Mapping (Grad-CAM) technology cannot effectively resist the interference of large-scale noise. In this article, an optimization of the deep CNN model was proposed by incorporating the Dropkey and Dropout (as a comparison) algorithm. Compared with Grad-CAM, the improved Grad-CAM based on Dropkey applies an attention mechanism to the feature map before calculating the gradient, which can introduce randomness and eliminate some areas by applying a mask to the attention score. Experimental results show that the optimized Grad-CAM deep CNN model based on the Dropkey algorithm can effectively resist large-scale noise interference and achieve accurate localization of image features. For instance, under the interference of a noise variance of 0.6, the Dropkey-enhanced ResNet50 model achieves a confidence level of 0.878 in predicting results, while the other two models exhibit confidence levels of 0.766 and 0.481, respectively. Moreover, it exhibits excellent performance in visualizing tasks related to image features such as distortion, low contrast, and small object characteristics. Furthermore, it has promising prospects in practical computer vision applications. For instance, in the field of autonomous driving, it can assist in verifying whether deep learning models accurately understand and process crucial objects, road signs, pedestrians, or other elements in the environment.
Soft-Sensing Regression Model: From Sensor to Wafer Metrology Forecasting
Authors: Fan Angzhi , Huang Yu , Xu Fei , Bom Sthitie
Published: October 2023

The semiconductor industry is one of the most technology-evolving and capital-intensive market sectors. Effective inspection and metrology are necessary to improve product yield, increase product quality and reduce costs. In recent years, many types of semiconductor manufacturing equipments have been equipped with sensors to facilitate real-time monitoring of the production processes. These production-state and equipment-state sensor data provide an opportunity to practice machine-learning technologies in various domains, such as anomaly/fault detection, maintenance scheduling, quality prediction, etc. In this work, we focus on the soft-sensing regression problem in metrology systems, which uses sensor data collected during wafer processing steps to predict impending inspection measurements that used to be measured in wafer inspection and metrology systems. We proposed a regressor based on Long Short-term Memory network and devised two distinct loss functions for the purpose of the training model. Although the assessment of our prediction errors by engineers is subjective, a novel piece-wise evaluation metric was introduced to evaluate model accuracy in a mathematical way. Our experimental results showcased that the proposed model is capable of achieving both accurate and early prediction across various types of inspections in complicated manufacturing processes.