bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity of Bacteria Associated with Guts and Gonads in Three Spider Species and Potential Transmission Pathways of Microbes within the Same Spider Host
Authors: Liu Yue , Liu Jia , Zhang Xiaopan , Yun Yueli
Published: September 2023

Microbial symbiosis plays a crucial role in the ecological and evolutionary processes of animals. It is well known that spiders, with their unique and diverse predatory adaptations, assume an indispensable role in maintaining ecological balance and the food chain. However, our current understanding of spider microbiomes remains relatively limited. The gut microbiota and gonad microbiota of spiders can both potentially influence their physiology, ecology, and behavior, including aspects such as digestion, immunity, reproductive health, and reproductive behavior. In the current study, based on high-throughput sequencing of the 16S rRNA V3 and V4 regions, we detected the gut and gonad microbiota communities of three spider species captured from the same habitat, namely, Eriovixia cavaleriei, Larinioides cornutus, and Pardosa pseudoannulata. In these three species, we observed that, at the phylum level classification, the gut and gonad of E. cavaleriei are primarily composed of Proteobacteria, while those of L. cornutus and P. pseudoannulata are primarily composed of Firmicutes. At the genus level of classification, we identified 372 and 360 genera from the gut and gonad bacterial communities. It is noteworthy that the gut and gonad bacterial flora of E. cavaleriei and L. cornutus were dominated by Wolbachia and Spiroplasma. Results show that there were no differences in microbial communities between females and males of the same spider species. Furthermore, there is similarity between the gut and ovary microbial communities of female spiders, implying a potential avenue for microbial transmission between the gut and gonad within female spiders. By comprehensively studying these two microbial communities, we can establish the theoretical foundation for exploring the relationship between gut and gonad microbiota and their host, as well as the mechanisms through which microbes exert their effects.
Screening and Functional Analyses of Novel Cecropins from Insect Transcriptome
Authors: Guo Lizhen , Tang Min , Luo Shiqi , Zhou Xin
Published: September 2023

Antibiotic resistance is a significant and growing threat to global public health. However, antimicrobial peptides (AMPs) have shown promise as they exhibit a broad spectrum of antibacterial activities with low potential for resistance development. Insects, which inhabit a wide range of environments and are incredibly diverse, remain largely unexplored as a source of novel AMPs. To address this, we conducted a screening of the representative transcriptomes from the 1000 Insect Transcriptome Evolution (1KITE) dataset, focusing on the homologous reference genes of Cecropins, the first identified AMPs in insects known for its high efficiency. Our analysis identified 108 Cecropin genes from 105 insect transcriptomes, covering all major hexapod lineages. We validated the gene sequences and synthesized mature peptides for three identified Cecropin genes. Through minimal inhibition concentration and agar diffusion assays, we confirmed that these peptides exhibited antimicrobial activities against Gram-negative bacteria. Similar to the known Cecropin, the three Cecropins adopted an alpha-helical conformation in membrane-like environments, efficiently disrupting bacterial membranes through permeabilization. Importantly, none of the three Cecropins demonstrated cytotoxicity in erythrocyte hemolysis tests, suggesting their safety in real-world applications. Overall, this newly developed methodology provides a high-throughput bioinformatic pipeline for the discovery of AMP, taking advantage of the expanding genomic resources available for diverse organisms.
Consumptive and Non-Consumptive Uses of Water Beetles (Aquatic coleopterans) in Sub-Saharan Traditional Rituals
Authors: Mnisi Lucky Nhlanhla , Zondi Nompumelelo , Pikirayi Innocent
Published: September 2023

The use of wild animals in customary rituals and as a sustenance resource is a longstanding tradition within sub-Saharan Africa. The emergence of commercial trade, has, however, created unattainable demands and has led to the overexploitation of animals. These demands are threatening the conservation of animal species exploited in this trade. Comparatively little research effort has been dedicated to invertebrate species, and, specifically, their non-commercial uses. We explored the uses of water beetles in traditional rituals. We investigate the extent to which each of the non-commercial uses of water beetles exhibits consumptive and non-consumptive use features. The concepts are contested as their application for describing human–animal interactions has been challenged because of insufficient physiological and conservation data on the implications for animals of such interactions. The inadequacy of the available data pertaining to the use of animal resources was particularly pronounced. Most research efforts are skewed towards vertebrates at the expense of invertebrates. Regardless, the study shows that most non-commercial exploitation and uses of water beetles were mainly non-destructive and, if consumptive, the uses could be described as mainly non-lethal consumptive or sub-lethal consumptive. Rituals that could be described as lethal-consumptive comprised a smaller fraction of the uses of water beetles.
Cysteine-Altering NOTCH3 Variants Are Associated with an Increased Risk of Autoimmune Diseases
Authors: Rieder Emily , Li Jiang , Rodriguez-Flores Juan L. , Taimur Malik Muhammad , Abedi Vida , Zand Ramin
Published: September 2023

Autoimmune conditions have been reported among patients with cysteine-altering NOTCH3 variants and CADASIL. This study aimed to investigate the occurrence of autoimmune illnesses and markers of inflammation in such populations. Cases were identified who had a NOTCH3 cysteine-altering variant from the Geisinger MyCode® Community Health Initiative (MyCode®). We further performed external validation using the UK Biobank cohort. A cohort of 121 individuals with a NOTCH3 cysteine-altering variant from MyCode® was compared to a control group with no non-synonymous variation in NOTCH3 (n = 184). Medical records were evaluated for inflammatory markers and autoimmune conditions, which were grouped by the organ systems involved. A similar analysis was conducted using data from the UK Biobank (n~450,000). An overall increase in inflammatory markers among participants with a NOTCH3 cysteine-altering variant was observed when compared to an age- and sex-matched MyCode® control group (out of participants with laboratory testing: 50.9% versus 26.7%; p = 0.0047; out of total participants: 23.1% versus 10.9%; p = 0.004). Analysis of UK Biobank data indicated any autoimmune diagnosis (1.63 [1.14, 2.09], p= 2.665 × 10−3) and multiple sclerosis (3.42 [1.67, 6.02], p = 9.681 × 10−4) are associated with a NOTCH3 cysteine-altering variant in any domain. Our findings suggest a possible association between NOTCH3 cysteine-altering variants and autoimmune conditions.
Foot Pain and Disability in Women with Rheumatoid Arthritis, Ehlers–Danlos Syndrome and Systemic Lupus Erythematosus: Relationship with Quality of Life
Authors: Palomo-Toucedo Inmaculada C. , Domínguez-Maldonado Gabriel , Reina-Bueno María , Vázquez-Bautista María del Carmen , Ramos-Ortega Javier , Castillo-López José Manuel , Munuera-Martínez Pedro V.
Published: September 2023

The aim of this work was to investigate the relationship foot pain and foot disability have with HRQoL in groups of women with RA, SLE and EDS, in comparison with a control group. A cross-sectional study was carried out with females with one of these conditions and a control group. The SF-12 questionnaire was used to collect data about quality of life. The type of foot was classified according to the footprint and the foot posture index. A total of 156 patients and 47 controls participated in the study (N = 203). Neither pain nor foot posture were different between groups. The physical and mental components of SF-12 were worse in rheumatoid arthritis and Ehlers–Danlos syndrome patients, and the physical component was worse in systemic lupus erythematosus patients, compared to controls. A significant difference was also observed in the mental component between systemic lupus erythematosus and Ehlers–Danlos syndrome patients, the latter having the lowest values among the groups. We can conclude that women with rheumatoid arthritis, Ehlers–Danlos syndrome, systemic lupus erythematosus and foot pain perceive a worse quality of life. There are no significant changes in foot posture. Pain and health-related quality of life are independent of foot posture.
Patterns of Cough Medication Prescribing among Patients with Chronic Cough in Florida: 2012–2021
Authors: Yang Seonkyeong , Huang Shu , Hincapie-Castillo Juan M. , Ke Xuehua , Ding Helen , Schelfhout Jonathan , Sher Mandel R. , Jones Bobby , Wilson Debbie L. , Lo-Ciganic Wei-Hsuan
Published: September 2023

Among patients with chronic cough (CC) in the 2012–2021 statewide OneFlorida Clinical Research Consortium database, we examined trends in cough medication (CM) prescribing prevalence over time in repeated cross-sectional analyses and identified distinct CM utilization trajectories using group-based trajectory modeling (GBTM) in a retrospective cohort study. Among eligible adults (≥18 years) without cancer/benign respiratory tumor diagnoses, we identified CC patients and non-CC patients with any cough-related diagnosis. In the GBTM analysis, we calculated the number of monthly prescriptions for any CMs (excluding gabapentinoids) during the 12 months from the first qualifying cough event to identify distinct utilization trajectories. From 2012 to 2021, benzonatate (9.6% to 26.1%), dextromethorphan (5.2% to 8.6%), and gabapentinoid (5.3% to 14.4%) use increased among CC patients, while opioid antitussive use increased from 2012 to 2015 and decreased thereafter (8.4% in 2012, 14.7% in 2015, 6.7% in 2021; all p < 0.001). Of 15,566 CC patients and 655,250 non-CC patients identified in the GBTM analysis, CC patients had substantial burdens of respiratory/non-respiratory comorbidities and healthcare service and concomitant medication use compared to non-CC patients. Among CC patients, GBTM identified three distinct CM utilization trajectories: (1) no CM use (n = 11,222; 72.1%); (2) declining CM use (n = 4105; 26.4%); and (3) chronic CM use (n = 239; 1.5%). CC patients in Florida had limited CM use with increasing trends in use of benzonatate, dextromethorphan, and gabapentinoids and a decreasing trend in opioid antitussive use. CC patients, particularly with chronic prescription CM use, experienced substantial disease burden.
Multimodal Imaging of Cancer Therapy-Related Cardiac Dysfunction in Breast Cancer—A State-of-the-Art Review
Authors: Cronin Michael , Seher Mehreen , Arsang-Jang Shahram , Lowery Aoife , Kerin Michael , Wijns William , Soliman Osama
Published: September 2023

Background: This review focuses on multimodality imaging of cardiotoxicity in cancer patients, with the aim of evaluating the effectiveness of different techniques in detecting and monitoring cardiac changes associated with cancer therapy. Methods: Eight studies were included in the review, covering various imaging modalities such as cardiac magnetic resonance imaging, echocardiography, and multigated acquisition scanning. Results: Cardiac magnetic resonance imaging emerged as the most definitive modality, offering real-time detection, comprehensive assessment of cardiac function, the ability to detect early myocardial changes, and superior detection of cardiotoxicity when compared to the other imaging modalities. The studies also emphasize the importance of parameters such as left ventricular ejection fraction and global longitudinal strain in assessing cardiac function and predicting cardiotoxicity. Conclusion: Due to the common use of HER2 agents and anthracyclines within the breast cancer population, the LVEF as a critical prognostic measurement for assessing heart health and estimating the severity of left-sided cardiac malfunction is a commonly used endpoint. CTRCD rates differed between imaging modalities, with cardiac MRI the most sensitive. The use of multimodal cardiac imaging remains a nuanced area, influenced by local availability, the clinical question at hand, body habits, and medical comorbidities. All of the imaging modalities listed have a role to play in current care; however, focus should be given to increasing the provision of cardiac MRI for breast cancer patients in the future to optimize the detection of CTRCD and patient outcomes thereafter.
Influence of Aortic Valve Stenosis and Wall Shear Stress on Platelets Function
Authors: Bańka Paweł , Wybraniec Maciej , Bochenek Tomasz , Gruchlik Bartosz , Burchacka Aleksandra , Swinarew Andrzej , Mizia-Stec Katarzyna
Published: September 2023

Aortic valve stenosis (AS) is a common heart valve disease in the elderly population, and its pathogenesis remains an interesting area of research. The degeneration of the aortic valve leaflets gradually progresses to valve sclerosis. The advanced phase is marked by the presence of extracellular fibrosis and calcification. Turbulent, accelerated blood flow generated by the stenotic valve causes excessive damage to the aortic wall. Elevated shear stress due to AS leads to the degradation of high-molecular weight multimers of von Willebrand factor, which may involve bleeding in the mucosal tissues. Conversely, elevated shear stress has been associated with the release of thrombin and the activation of platelets, even in individuals with acquired von Willebrand syndrome. Moreover, turbulent blood flow in the aorta may activate the endothelium and promote platelet adhesion and activation on the aortic valve surface. Platelets release a wide range of mediators, including lysophosphatidic acid, which have pro-osteogenic effects in AS. All of these interactions result in blood coagulation, fibrinolysis, and the hemostatic process. This review summarizes the current knowledge on high shear stress-induced hemostatic disorders, the influence of AS on platelets and antiplatelet therapy.
HIV/Tuberculosis Coinfection in Pregnancy and the Postpartum Period
Authors: Yilma Addis , Bailey Hannah , Karakousis Petros , Karanika Styliani
Published: September 2023

The convergence of Human Immunodeficiency Virus (HIV) and tuberculosis (TB) represents a considerable global public health challenge. The concurrent infection of HIV and TB in pregnant women not only intensifies the transmission of HIV from mother to fetus but also engenders adverse outcomes for maternal health, pregnancy, and infant well-being, necessitating the implementation of integrated strategies to effectively address and manage both diseases. In this article, we review the pathophysiology, clinical presentation, treatment, and management of HIV/TB coinfection during pregnancy, the postpartum period, and lactation and highlight the differences compared to the general population.
Exploring the Accuracy and Consistency of a School Readiness Assessment Tool for Preschoolers: Reliability, Validity and Measurement Invariance Analysis
Authors: Józsa Krisztián , Oo Tun Zaw , Borbélyová Diana , Zentai Gabriella
Published: September 2023

This study focuses on examining the psychometric properties of the DIFER test, a widely used assessment tool for measuring school readiness. DIFER, which stands for Diagnostic Assessment Systems for Development, has gained prominence in Hungary and some European countries as an effective means of evaluating children’s readiness for school. By investigating the reliability and validity of the DIFER test, this study aims to enhance the understanding of the suitability of the DIFER test for cross-cultural and longitudinal studies in assessing school readiness. Conducted as a survey study, the research involved 3050 Hungarian students from Slovakia and Hungary. Employing Rasch analysis and multi-group confirmatory factor analysis (MG-CFA) aid in verifying the precision of the DIFER test as a valuable assessment instrument for determining school readiness. The results revealed a strong alignment between the difficulty level of the test and students’ actual abilities, demonstrating its reliability and validity. Importantly, the analysis found measurement invariance across various factors, including country, gender, and age. This indicates the consistent performance of the DIFER test in assessing school readiness across diverse groups. However, mean differences in latent abilities were observed among different age groups, indicating that older students exhibited notably higher proficiency in pre-mathematical skills compared to their younger counterparts. The findings offer valuable insights to educators, providing a reliable tool for assessing school readiness and identifying areas for improvement.
Correlations between Petroleum Reservoir Fluid Properties and Amount of Evolved and Dissolved Natural Gas: Case Study of Transgressive–Regressive-Sequence Sedimentary Rocks
Authors: Kamal Ibtisam , Salih Namam M. , Martyushev Dmitriy A.
Published: September 2023

It is well recognized that PVT data are essential in oil and gas production facilities as well as in the determination of the reservoir fluid composition in reservoir engineering calculations. In the current work, the studied borehole is located in Tawke oilfield in the High Folded Zone. The structural geology and lithological facies of rocks are studied and found to comprise fine crystalline dolomite and anhydrite interbedded with claystone and dolomite. In addition, the practical PVT data of black oil from Tawke oilfield, Zakho, from reservoirs to transgressive–regressive cycles, are studied. The PVT data are investigated to derive the empirical models that rule and correlate the properties of the reservoir fluids in terms of the amount of natural gas (methane, ethane, and propane) dissolved in reservoir fluids and evolving from the wells. The characteristics of the reservoir fluid, including °API, viscosity at reservoir pressure and bubble-point pressure, reservoir pressure and temperature, gas–oil ratio (GOR), coefficient of compressibility at reservoir pressure, gross heating value, and sample depth, are correlated. The lithological part reveals that the carbonate and some clastic rock facies are conducive to enhancing natural gas adsorption. The reservoir fluid properties show adverse effects on the amount of natural gas constituents evolving from the wells, while it shows positive effects on the dissolved reservoir fluids. The estimated empirical correlations can help indicate the quantity of natural gas that is dissolved in reservoir fluids and liberated from the wells depending on the characteristics of the reservoir. In addition, they can be used in numerical simulators to predict oil well performance.
Experimental Investigation of Surface Waves Effect on a Ducted Twin Vertical Axis Tidal Turbine
Authors: Moreau Martin , Germain Grégory , Maurice Guillaume
Published: September 2023

The cost effective design of tidal turbines requires a good estimation of the loading cycles and their extrema that are related to the unsteady fluctuation of the current velocity. Apart from the ambient turbulence, the main source of velocity fluctuation is the presence of surface waves. In the present study, we analyse the effect of waves propagating against the current on the performance and the loads of a twin vertical axis tidal turbine by an experimental approach at a 1/20 scale. Overall, the results show little or no effect of the waves on the average power and loads compared to the conditions with current only, but a significant impact on their standard deviation that rises linearly with the amplitude of the waves. The drag, lift, and pitching moment show extended ranges up to 7.5 times higher and extreme values exceedance by 60 to 100% with irregular waves compared to the conditions without waves. That load and power fluctuation increase is totally due to the presence of waves as the coherence function between the rotor torque or the loads and the velocity spectra exceed 0.5 on the whole wave frequency bandwidth. The results also reveal a rotational sampling of the waves by the rotors that had also been observed on horizontal axis turbines. From a structural design point of view, the authors recommend conducting tests in irregular wave conditions as both the load ranges and extreme values are 1.5 to 2 times higher than those encountered with regular waves of the same significant height and period.
U-Net-LSTM: Time Series-Enhanced Lake Boundary Prediction Model
Authors: Yin Lirong , Wang Lei , Li Tingqiao , Lu Siyu , Tian Jiawei , Yin Zhengtong , Li Xiaolu , Zheng Wenfeng
Published: September 2023

Change detection of natural lake boundaries is one of the important tasks in remote sensing image interpretation. In an ordinary fully connected network, or CNN, the signal of neurons in each layer can only be propagated to the upper layer, and the processing of samples is independent at each moment. However, for time-series data with transferability, the learned change information needs to be recorded and utilized. To solve the above problems, we propose a lake boundary change prediction model combining U-Net and LSTM. The ensemble of LSTMs helps to improve the overall accuracy and robustness of the model by capturing the spatial and temporal nuances in the data, resulting in more precise predictions. This study selected Lake Urmia as the research area and used the annual panoramic remote sensing images from 1996 to 2014 (Lat: 37°00′ N to 38°15′ N, Lon: 46°10′ E to 44°50′ E) obtained by Google Earth Professional Edition 7.3 software as the research data set. This model uses the U-Net network to extract multi-level change features and analyze the change trend of lake boundaries. The LSTM module is introduced after U-Net to optimize the predictive model using historical data storage and forgetting as well as current input data. This method enables the model to automatically fit the trend of time series data and mine the deep information of lake boundary changes. Through experimental verification, the model’s prediction accuracy for lake boundary changes after training can reach 89.43%. Comparative experiments with the existing U-Net-STN model show that the U-Net-LSTM model used in this study has higher prediction accuracy and lower mean square error.
Similarity Transformations and Nonlocal Reduced Integrable Nonlinear Schrödinger Type Equations
Authors: Cheng Li , Ma Wen-Xiu
Published: September 2023

We present three reduced integrable hierarchies of nonlocal integrable nonlinear Schrödinger-type equations, starting from a given vector integrable hierarchy generated from a matrix Lie algebra of B type. The basic tool is the zero curvature formulation. Three similarity transformations are taken to keep the invariance of the involved zero curvature equations. The key is to formulate a matrix solution to a reduced stationary zero curvature equation such that the zero curvature formulation works for a reduced case.
On the Properties of λ-Prolongations and λ-Symmetries
Authors: Li Wenjin , Li Xiuling , Pang Yanni
Published: September 2023

In this paper, (1) We show that if there are not enough symmetries and λ-symmetries, some first integrals can still be obtained. And we give two examples to illustrate this theorem. (2) We prove that when X is a λ-symmetry of differential equation field Γ, by multiplying Γ a function μ defineded on Jn−1M, the vector fields μΓ can pass to quotient manifold Q by a group action of λ-symmetry X. (3) If there are some λ-symmetries of equation considered, we show that the vector fields from their linear combination are symmetries of the equation under some conditions. And if we have vector field X defined on Jn−1M with first-order λ-prolongation Y and first-order standard prolongations Z of X defined on JnM, we prove that gY cannot be first-order λ-prolonged vector field of vector field gX if g is not a constant function. (4) We provide a complete set of functionally independent (n−1) order invariants for V(n−1) which are n−1th prolongation of λ-symmetry of V and get an explicit n−1 order reduced equation of explicit n order ordinary equation considered. (5) Assume there is a set of vector fields Xi,i=1,...,n that are in involution, We claim that under some conditions, their λ-prolongations also in involution.
Gibbs Distribution and the Repairman Problem
Authors: Chetouani Hassan , Limnios Nikolaos
Published: September 2023

In this paper, we obtain weak convergence results for a family of Gibbs measures depending on the parameter θ>0 in the following form dPθ(x)=Zθexp−Hθ(x)/θdQ(x), where we show that the limit distribution is concentrated in the set of the global minima of the limit Gibbs potential. We also give an explicit calculus for the limit distribution. Here, we use the above as an alternative to Lyapunov’s function or to direct methods for stationary probability convergence and apply it to the repairman problem. Finally, we illustrate this method with a numerical example.
A Boundary-Element Analysis of Crack Problems in Multilayered Elastic Media: A Review
Authors: Lan Lei , Zhou Jiaqi , Xu Wanrong , Long Gongbo , Xiao Boqi , Xu Guanshui
Published: September 2023

Crack problems in multilayered elastic media have attracted extensive attention for years due to their wide applications in both a theoretical analysis and practical industry. The boundary element method (BEM) is widely chosen among various numerical methods to solve the crack problems. Compared to other numerical methods, such as the phase field method (PFM) or the finite element method (FEM), the BEM ensures satisfying accuracy, broad applicability, and satisfactory efficiency. Therefore, this paper reviews the state-of-the-art progress in a boundary-element analysis of the crack problems in multilayered elastic media by concentrating on implementations of the two branches of the BEM: the displacement discontinuity method (DDM) and the direct method (DM). The review shows limitation of the DDM in applicability at first and subsequently reveals the inapplicability of the conventional DM for the crack problems. After that, the review outlines a pre-treatment that makes the DM applicable for the crack problems and presents a DM-based method that solves the crack problems more efficiently than the conventional DM but still more slowly than the DDM. Then, the review highlights a method that combines the DDM and the DM so that it shares both the efficiency of the DDM and broad applicability of the DM after the pre-treatment, making it a promising candidate for an analysis of the crack problems. In addition, the paper presents numerical examples to demonstrate an even faster approximation with the combined method for a thin layer, which is one of the challenges for hydraulic-fracturing simulation. Finally, the review concludes with a comprehensive summary and an outlook for future study.
Random Forest Regression in Predicting Students’ Achievements and Fuzzy Grades
Authors: Doz Daniel , Cotič Mara , Felda Darjo
Published: September 2023

The use of fuzzy logic to assess students’ knowledge is not a completely new concept. However, despite dealing with a large quantity of data, traditional statistical methods have typically been the preferred approach. Many studies have argued that machine learning methods could offer a viable alternative for analyzing big data. Therefore, this study presents findings from a Random Forest (RF) regression analysis to understand the influence of demographic factors on students’ achievements, i.e., teacher-given grades, students’ outcomes on the national assessment, and fuzzy grades, which were obtained as a combination of the two. RF analysis showed that demographic factors have limited predictive power for teacher-assigned grades, unlike INVALSI scores and fuzzy grades. School type, macroregion, and ESCS are influential predictors, whereas gender and origin have a lesser impact. The study highlights regional and socio-economic disparities, influencing both student outcomes and fuzzy grades, underscoring the need for equitable education. Unexpectedly, gender’s impact on achievements is minor, possibly due to gender-focused policies. Although the study acknowledges limitations, its integration of fuzzy logic and machine learning sets the foundation for future research and policy recommendations, advocating for diversified assessment approaches and data-driven policymaking.
Evaluation of Changes in Circulating Cell-Free DNA as an Early Predictor of Response to Chemoradiation in Rectal Cancer—A Pilot Study
Authors: Ong Wee Liam , Lunca Sorinel , Morarasu Stefan , Musina Ana-Maria , Puscasu Alina , Iacob Stefan , Iftincai Irina , Marinca Andreea , Ivanov Iuliu , Roata Cristian Ena , Velenciuc Natalia , Dimofte Gabriel
Published: September 2023

Background and Objectives: The objective of this study was to investigate quantitative changes in cell-free DNA (cfDNA) found in the bloodstream of patients with locally advanced rectal cancer who received neoadjuvant long-course chemoradiation, assuming a change in DNA fragments release during therapeutic stress. Materials and Methods: This was a prospective observational study that involved 49 patients who had three distinct pathologies requiring neoadjuvant chemoradiation: 18 patients with breast cancer, 18 patients with cervical cancer, and 13 patients with rectal cancer. Both breast cancer and cervical cancer patients were used as a control groups. Breast cancer patients were used as a control group as irradiation targeted healthy tissue after the tumor resection (R0), while cervical cancer patients were used as a control group to evaluate the effect of chemoradiation regarding cfDNA in a different setting (squamous cell carcinomas) and a different tumor burden. Rectal cancer patients were the study group, and were prospectively evaluated for a correlation between fragmentation of cfDNA and late response to chemoradiation. Blood samples were collected before the initiation of treatment and after the fifth radiation dose delivery. cfDNA was quantified in peripheral blood and compared with the patients’ clinicopathological characteristics and tumor volume. Conclusion: Thirteen patients with locally advanced rectal cancer (T3/T4/N+/M0) were included in the study, and all of them had their samples analyzed. Eight were male (61.54%) and five were female (38.46%), with an average age of 70.85 years. Most of the patients had cT3 (53.85%) or cT4 (46.15%) tumors, and 92.31% had positive lymph nodes (N2–3). Of the thirteen patients, only six underwent surgery, and one of them achieved a pathological complete response (pCR). The mean size of the tumor was 122.60 mm3 [35.33–662.60 mm3]. No significant correlation was found between cfDNA, tumor volume, and tumor regression grade. cfDNA does not seem to predict response to neoadjuvant chemoradiotherapy and it is not correlated to tumor volume or tumor regression grade.
Curcumin Nanoemulsion: Unveiling Cardioprotective Effects via ACE Inhibition and Antioxidant Properties in Hypertensive Rats
Authors: Ishaq Mohd , Khan Mohemmed Faraz , Verma Garima , Rathi Akshoo , Adil Mohammad , Faizan Mohammad , Najmi Abul Kalam , Akhtar Mohd , Al kamaly Omkulthom , Alshawwa Samar Zuhair , Shahat Abdelaaty A. , Alhalmi Abdulsalam
Published: September 2023

Background and Objectives: Curcumin, derived from Curcuma longa, is a well-known traditional medicinal compound recognized for its therapeutic attributes. Nevertheless, its efficacy is hampered by limited bioavailability, prompting researchers to explore the application of nanoemulsion as a potential alternative. Materials and Methods: This study delves into the antihypertensive effects of curcumin nanoemulsion (SNEC) by targeting the renin-angiotensin-aldosterone system (RAAS) and oxidative stress in deoxycorticosterone acetate (DOCA) salt-induced hypertensive rats. To gauge the cardio-protective impact of SNEC in DOCA salt-induced hypertension, molecular docking was undertaken, uncovering curcumin’s high affinity and adept binding capabilities to the active site of angiotensin-converting enzyme (ACE). Additionally, the investigation employed uninephrectomized rats to assess hemodynamic parameters via an AD instrument. Serum ACE, angiotensin II, blood urea nitrogen (BUN), and creatinine levels were quantified using ELISA kits, while antioxidant parameters were evaluated through chemical assays. Result: The outcomes of the molecular docking analysis revealed robust binding of curcumin to the ACE active site. Furthermore, oral administration of SNEC significantly mitigated systolic, diastolic, and mean arterial blood pressure in contrast to the DOCA-induced hypertensive group. SNEC administration also led to a reduction in left ventricular end-diastolic pressure (LVEDP) and an elevation in the maximum rate of left ventricular pressure rise (LV (dP/dt) max). Moreover, SNEC administration distinctly lowered serum levels of ACE and angiotensin II compared to the hypertensive DOCA group. Renal markers, including serum creatinine and BUN, displayed a shift toward normalized levels with SNEC treatment. Additionally, SNEC showcased potent antioxidant characteristics by elevating reduced glutathione, catalase, and superoxide dismutase levels, while decreasing the concentration of thiobarbituric acid reactive substances. Conclusions: Collectively, these findings underscore that curcumin nanoemulsion exerts noteworthy cardio-protective effects through ACE activity inhibition and remarkable antioxidant properties.
A Fast Transient Adaptive On-Time Controlled BUCK Converter with Dual Modulation
Authors: Sun Mengyuan , Chen Chufan , Wang Leiyi , Xie Xinling , Wang Yuhang , Xu Min
Published: September 2023

This paper proposed a fully integrated adaptive on-time (AOT) controlled buck converter with fast load transient. An adaptive on-time generator is presented to stabilize the output frequency. To enhance the light load efficiency, the converter could transfer from the pulse width modulation (PWM) to pulse skip modulation (PSM) as the load current decreases. The buck converter can switch between these two modulation modes adaptively with the assistance of a zero current detection circuit. Implemented in the TSMC 0.18 µm BCD (BiCMOS/DMOS) process, the proposed buck converter works with an input voltage ranging from 5.5 to 15 V, an output voltage ranging from 0.5 to 5 V, and an output load ranging up to 5 A. The experimental results show that based on the dual modulation adaptive on-time controlled mode, the transient recovery time from light to heavy load and from heavy load to light load is 13 µs and 15 µs, respectively. An overshot voltage of 57 mV and an undershot voltage of 53 mV are also achieved.
Biodeterioration and Chemical Corrosion of Concrete in the Marine Environment: Too Complex for Prediction
Authors: Gaylarde Christine C. , Ortega-Morales Benjamin Otto
Published: September 2023

Concrete is the most utilized construction material worldwide. In the marine environment, it is subject to chemical degradation through reactions with chloride (the most important ion), and sulfate and magnesium ions in seawater, and to biodeterioration resulting from biological (initially microbiological) activities, principally acid production. These two types of corrosions are reviewed and the failure of attempts to predict the degree of deterioration resulting from each is noted. Chemical (abiotic) corrosion is greatest in the splash zone of coastal constructions, while phenomenological evidence suggests that biodeterioration is greatest in tidal zones. There have been no comparative experiments to determine the rates and types of microbial biofilm formation in these zones. Both chemical and microbiological concrete deteriorations are complex and have not been successfully modeled. The interaction between abiotic corrosion and biofilm formation is considered. EPS can maintain surface hydration, potentially reducing abiotic corrosion. The early marine biofilm contains relatively specific bacterial colonizers, including cyanobacteria and proteobacteria; these change over time, producing a generic concrete biofilm, but the adhesion of microorganisms to concrete in the oceans has been little investigated. The colonization of artificial reefs is briefly discussed. Concrete appears to be a relatively prescriptive substrate, with modifications necessary to increase colonization for the required goal of increasing biological diversity.
Thermal Inactivation of Hepatitis E Virus in Pork Products Estimated with a Semiquantitative Infectivity Assay
Authors: Stunnenberg Melissa , Huizen Suzanne C. van , Swart Arno , Lodder Willemijn J. , Boxman Ingeborg L. A. , Rutjes Saskia A.
Published: September 2023

Hepatitis E virus genotype 3 (HEV-3) is a food-borne pathogen causative of hepatitis E infections in humans. In Europe, HEV-3 is mainly transmitted through the consumption of raw or undercooked pork. In order to determine the effectiveness of control measures that can be taken in the industry or by the consumer, it is pivotal to determine the infectivity of HEV present in pork products after thermal food-processing steps. First, we implemented a method for the detection of infectious HEV-3c and HEV-3e in a cell culture medium and in extracts from inoculated pork products. Next, we investigated the effect of the thermal inactivation of HEV by mimicking food-processing steps specific for dried sausage and liver homogenate matrices. After four weeks, HEV-inoculated dried sausage subjected to 21 °C or lower temperatures was still infectious. For the liver homogenate, the highest HEV-3c/e inactivation of the conditions tested was observed at 71 °C for five min or longer. Finally, our method was able to successfully detect and estimate viral loads of infectious HEV in naturally infected pig livers. Our data provide a basis for the future use of the quantitative microbial risk assessment of infectious HEV in pork products that are subjected to thermal food processing steps.
One-Pot Synthesis of 3,4-Dihydrocoumarins via C-H Oxidation/Conjugate Addition/Cyclization Cascade Reaction
Authors: Kim Dae Young
Published: September 2023

The 3,4-dihydrocoumarin derivatives were obtained from 2-alkyl phenols and oxazolones via C–H oxidation and cyclization cascade in the presence of silver oxide (Ag2O) and p-toluenesulfonic acid as a Brønsted acid catalyst. This approach provides a one-pot strategy to synthesize the multisubstituted 3,4-dihydrocoumarins with moderate to high yields (64–81%) and excellent diastereoselectivity (>20:1).
Fingerprint Analysis of Volatile Flavor Compounds in Crassostrea gigas of Different Ploidy and Gender under High-Temperature Incubation
Authors: Sun Youmei , Fu Jingjing , Zhang Enshuo , Dong Luyao , Cui Xuebo , Sun Yanan , Wang Zhizhong , Feng Yanwei , Li Bin , Xu Xiaohui , Luo Qihao , Wang Weijun , Yang Jianmin
Published: September 2023

In this study, diploid, triploid, and tetraploid Crassostrea gigas samples were subjected to gas chromatography and ion mobility (GC-IMS) to identify and analyze volatile compounds and flavor fingerprints under conditions of high-temperature incubation. The GC-IMS technology identified a total of 54 volatile components in C. gigas. The contents of 1-octen-3-ol, butyl pentanoate, p-methyl anisole, and 2-methyl-2-hepten-6-one in male oysters were significantly higher than in females, while the contents of phenylacetaldehyde, benzaldehyde, 2-ethyl-3-methylpyrazine, 2-ethylfuran, and 2,4-hexadienal in female oysters were significantly higher than in males. The contents of non-3-en-2-one-M and 1-pentanol in diploids were significantly higher than in triploids and tetraploids, while the content of 2,4-hexadienal in tetraploids was significantly higher than in diploids and tetraploids. The contents of ethyl acetate, ethyl-2-butenoate, and butanal in tetraploids were significantly higher than those in diploids and triploids. The results of a principal components analysis showed that different samples were relatively independently clustered, allowing the ability to distinguish different oyster samples. The chemical fingerprints of volatile compounds of C. gigas with different ploidy and gender under high-temperature incubation were established, and the volatile substance contours of C. gigas were visualized. The results provide a reference for distinguishing the ploidy and gender of C. gigas under conditions of high-temperature incubation.
The Nitric Oxide (NO) Donor Molsidomine Attenuates Memory Impairments Induced by the D1/D2 Dopaminergic Receptor Agonist Apomorphine in the Rat
Authors: Vartzoka Foteini , Ozenoglu Elif , Pitsikas Nikolaos
Published: September 2023

Several lines of evidence suggest that scarcity of the gaseous molecule nitric oxide (NO) is associated with the pathogenesis of schizophrenia. Therefore, compounds, such as NO donors, that can normalize NO levels might be of utility for the treatment of this pathology. It has been previously shown that the NO donor molsidomine attenuated schizophrenia-like behavioral deficits caused by glutamate hypofunction in rats. The aim of the current study was to investigate the efficacy of molsidomine and that of the joint administration of this NO donor with sub-effective doses of the non-typical antipsychotics clozapine and risperidone to counteract memory deficits associated with dysregulation of the brain dopaminergic system in rats. Molsidomine (2 and 4 mg/kg) attenuated spatial recognition and emotional memory deficits induced by the mixed dopamine (DA) D1/D2 receptor agonist apomorphine (0.5 mg/kg). Further, the joint administration of sub-effective doses of molsidomine (1 mg/kg) with those of clozapine (0.1 mg/kg) or risperidone (0.03 mg/kg) counteracted non-spatial recognition memory impairments caused by apomorphine. The present findings propose that molsidomine is sensitive to DA dysregulation since it attenuates memory deficits induced by apomorphine. Further, the current findings reinforce the potential of molsidomine as a complementary molecule for the treatment of schizophrenia.
Qualitative and Quantitative Comparison of Aromatic Oil Components and Antifungal Effects of Cymbopogon flexuosus Obtained with Supercritical CO2, Microwave–Ultrasonic, Steam Distillation, and Hydrodistillation Extraction Techniques
Authors: Jaradat Nidal
Published: September 2023

Cymbopogon flexuosus is a highly valued botanical species with significant applications in the food and food supplement industries, medicine, and cosmetics. The effects of four extraction techniques, supercritical CO2, microwave–ultrasonic, steam distillation, and hydrodistillation techniques, on the yield, phytochemical constituents, and antifungal activity against nine fungal species of Cymbopogon flexuosus aromatic oil (AO) were explored in this investigation. Gas chromatography connected with a mass spectrometry apparatus was employed for the qualitative and quantitative analyses of the investigated plant AOs. In addition, using the broth microdilution method, minimum inhibitory concentrations (MICs) were calculated for several fungi species. The supercritical CO2 method gave the highest yield of AO (11.62 ± 0.03 (w/w)) followed by the microwave–ultrasonic method (1.55 ± 0.05% (w/w)) and the steam distillation method (1.24 ± 0.04% (w/w)), while the hydrodistillation methods gave the lowest yield (1.17 ± 0.01 (w/w)). In addition, eighteen molecules were specified in the AOs obtained with the supercritical CO2, microwave–ultrasonic, steam distillation, and hydrodistillation techniques, which constituted 99.36, 98.6, 98.21, and 98.31% (v/v) of the total oils, respectively. Additionally, linalyl acetate was the trending molecule in the microwave–ultrasonic and steam distillation methods, representing 24.61 and 24.34% (v/v), respectively, while geranial was the dominant molecule in the AOs extracted with the hydrodistillation and supercritical CO2 extraction techniques (27.01 and 25.6% (v/v), respectively). The antifungal screening results revealed that the tested C. flexuosus AOs have potential antifungal effects against all the screened fungi species. The antifungal effect of the AOs extracted with the steam distillation and microwave–ultrasonic methods was remarkable compared with that of the commercial antifungal drug Fluconazole. However, the AOs extracted with these two methods have a more potent antifungal effect against Candida parapsilosis than that of Fluconazole with MICs of 3.13 ± 0.01, 3.13 ± 0.01, and 6.25 ± 0.91 µg/mL, respectively. The same effects were also observed against Trichophyton rubrum with MICs of 6.25 ± 0.91 µg/mL, respectively. The results of this investigation demonstrated that the steam distillation and microwave–ultrasonic methods are promising processes for the extraction of C. flexuosus AO with a potent antifungal effect. This may be an advantage for the utilization of C. flexuosus AO over some antifungal synthetic agents commonly utilized as medicines, preservatives, food additives, cosmetics, and nutrient supplements.
Exploring Natural Alkaloids from Brazilian Biodiversity as Potential Inhibitors of the Aedes aegypti Juvenile Hormone Enzyme: A Computational Approach for Vector Mosquito Control
Authors: Costa Renato Araújo da , Costa Andréia do Socorro Silva da , Rocha João Augusto Pereira da , Lima Marlon Ramires da Costa , Rocha Elaine Cristina Medeiros da , Nascimento Fabiana Cristina de Araújo , Gomes Anderson José Baia , Rego José de Arimatéia Rodrigues do , Brasil Davi do Socorro Barros
Published: September 2023

This study explores the potential inhibitory activity of alkaloids, a class of natural compounds isolated from Brazilian biodiversity, against the mJHBP enzyme of the Aedes aegypti mosquito. This mosquito is a significant vector of diseases such as dengue, zika, and chikungunya. The interactions between the ligands and the enzyme at the molecular level were evaluated using computational techniques such as molecular docking, molecular dynamics (MD), and molecular mechanics with generalized Born surface area (MMGBSA) free energy calculation. The findings suggest that these compounds exhibit a high binding affinity with the enzyme, as confirmed by the binding free energies obtained in the simulation. Furthermore, the specific enzyme residues that contribute the most to the stability of the complex with the compounds were identified: specifically, Tyr33, Trp53, Tyr64, and Tyr129. Notably, Tyr129 residues were previously identified as crucial in the enzyme inhibition process. This observation underscores the significance of the research findings and the potential of the evaluated compounds as natural insecticides against Aedes aegypti mosquitoes. These results could stimulate the development of new vector control agents that are more efficient and environmentally friendly.