bluuebunny/crossref_metadata_2025 huggingface dataset:
The Diverse Piscidin Repertoire of the European Sea Bass (Dicentrarchus labrax): Molecular Characterization and Antimicrobial Activities
Authors: Barroso Carolina , Carvalho Pedro , Carvalho Carla , Santarém Nuno , Gonçalves José F. M. , Rodrigues Pedro N. S. , Neves João V.
Published: June 2020

Fish rely on their innate immune responses to cope with the challenging aquatic environment, with antimicrobial peptides (AMPs) being one of the first line of defenses. Piscidins are a group of fish specific AMPs isolated in several species. However, in the European sea bass (Dicentrarchus labrax), the piscidin family remains poorly understood. We identified six different piscidins in sea bass, performed an in-depth molecular characterization and evaluated their antimicrobial activities against several bacterial and parasitic pathogens. Sea bass piscidins present variable amino acid sequences and antimicrobial activities, and can be divided in different sub groups: group 1, formed by piscidins 1 and 4; group 2, constituted by piscidins 2 and 5, and group 3, formed by piscidins 6 and 7. Additionally, we demonstrate that piscidins 1 to 5 possess a broad effect on multiple microorganisms, including mammalian parasites, while piscidins 6 and 7 have poor antibacterial and antiparasitic activities. These results raise questions on the functions of these peptides, particularly piscidins 6 and 7. Considering their limited antimicrobial activity, these piscidins might have other functional roles, but further studies are necessary to better understand what roles might those be.
An Overview of Coumarin as a Versatile and Readily Accessible Scaffold with Broad-Ranging Biological Activities
Authors: Annunziata Francesca , Pinna Cecilia , Dallavalle Sabrina , Tamborini Lucia , Pinto Andrea
Published: June 2020

Privileged structures have been widely used as an effective template for the research and discovery of high value chemicals. Coumarin is a simple scaffold widespread in Nature and it can be found in a considerable number of plants as well as in some fungi and bacteria. In the last years, these natural compounds have been gaining an increasing attention from the scientific community for their wide range of biological activities, mainly due to their ability to interact with diverse enzymes and receptors in living organisms. In addition, coumarin nucleus has proved to be easily synthetized and decorated, giving the possibility of designing new coumarin-based compounds and investigating their potential in the treatment of various diseases. The versatility of coumarin scaffold finds applications not only in medicinal chemistry but also in the agrochemical field as well as in the cosmetic and fragrances industry. This review is intended to be a critical overview on coumarins, comprehensive of natural sources, metabolites, biological evaluations and synthetic approaches.
β-Arrestin 2 and ERK1/2 Are Important Mediators Engaged in Close Cooperation between TRPV1 and µ-Opioid Receptors in the Plasma Membrane
Authors: Melkes Barbora , Markova Vendula , Hejnova Lucie , Novotny Jiri
Published: June 2020

The interactions between TRPV1 and µ-opioid receptors (MOR) have recently attracted much attention because these two receptors play important roles in pain pathways and can apparently modulate each other’s functioning. However, the knowledge about signaling interactions and crosstalk between these two receptors is still limited. In this study, we investigated the mutual interactions between MOR and TRPV1 shortly after their activation in HEK293 cells expressing these two receptors. After activation of one receptor we observed significant changes in the other receptor’s lateral mobility and vice versa. However, the changes in receptor movement within the plasma membrane were not connected with activation of the other receptor. We also observed that plasma membrane β-arrestin 2 levels were altered after treatment with agonists of both these receptors. Knockdown of β-arrestin 2 blocked all changes in the lateral mobility of both receptors. Furthermore, we found that β-arrestin 2 can play an important role in modulating the effectiveness of ERK1/2 phosphorylation after activation of MOR in the presence of TRPV1. These data suggest that β-arrestin 2 and ERK1/2 are important mediators between these two receptors and their signaling pathways. Collectively, MOR and TRPV1 can mutually affect each other’s behavior and β-arrestin 2 apparently plays a key role in the bidirectional crosstalk between these two receptors in the plasma membrane.
Experimental Models in Neovascular Age Related Macular Degeneration
Authors: Rastoin Olivia , Pagès Gilles , Dufies Maeva
Published: June 2020

Neovascular age-related macular degeneration (vAMD), characterized by the neo-vascularization of the retro-foveolar choroid, leads to blindness within few years. This disease depends on angiogenesis mediated by the vascular endothelial growth factor A (VEGF) and to inflammation. The only available treatments consist of monthly intravitreal injections of antibodies directed against VEGF or VEGF/VEGFB/PlGF decoy receptors. Despite their relative efficacy, these drugs only delay progression to blindness and 30% of the patients are insensitive to these treatments. Hence, new therapeutic strategies are urgently needed. Experimental models of vAMD are essential to screen different innovative therapeutics. The currently used in vitro and in vivo models in ophthalmic translational research and their relevance are discussed in this review.
How Crucial is the Functional Pit Organ for the Varroa Mite?
Authors: Nganso Beatrice T. , Mani Kannan , Altman Yam , Rafaeli Ada , Soroker Victoria
Published: June 2020

Olfaction as well as gustation, are essential for animal survival, allowing behavioral modulation according to environmental input. We focused our study on an obligate ecto-parasitic mite of honey bees, the Varroa destructor Anderson and Trueman (Parasitiformes, Mesostigmata, Varroidae). By mechanically blocking the main olfactory organ on Varroa forelegs by varnishing with nail polish, we were able to show that other sensory organs cannot significantly compensate chemosensory abilities required for mite’s host selection, identification as well as reproduction. In fact, we found that mites with blocked forelegs had a significantly lower ability to reach a host bee than those with varnished idiosoma and unvarnished control. Furthermore, fewer foreleg blocked mites were feeding on the nurse bees and their reproduction in the brood cells was significantly impaired. The inhibition of reproduction was also reflected in altered expression levels of vitellogenin and vitellogenin receptor genes in foreleg-blocked mites.
Feeding Preference of Crapemyrtle Bark Scale (Acanthococcus lagerstroemiae) on Different Species
Authors: Xie Runshi , Wu Bin , Dou Haijie , Liu Cuiyu , Knox Gary W. , Qin Hongmin , Gu Mengmeng
Published: June 2020

Crapemyrtle bark scale (CMBS; Acanthococcus lagerstroemiae) is an exotic pest species that causes aesthetic and economic damage to crapemyrtles and poses potential threats to other horticultural crops in the United States. Although previous studies reported the infestation of CMBS on several alternative hosts across multiple families in Asia, its potential threats to other documented alternative hosts remain elusive and yet to be confirmed. In this study, feeding preference studies of CMBS were conducted on forty-nine plant species and cultivars in 2016 and 2019, in order to gain insight into the expansion of CMBS distribution in the United States, as well as other regions of the world. The infestations of CMBS were confirmed on apple (Malus domestica), Chaenomeles speciosa, Disopyros rhombifolia, Heimia salicifolia, Lagerstroemia ‘Spiced Plum’, M. angustifolia, and twelve out of thirty-five pomegranate cultivars. However, the levels of CMBS infestation on these test plant hosts in this study is very low compared to Lagerstroemia, and may not cause significant damage. No sign of CMBS infestation was observed on Rubus ‘Arapaho’, R. ‘Navaho’, R. idaeus ‘Dorman Red’, R. fruticosus, B. microphylla var. koreana × B. sempervirens, B. harlandii, or D. virginiana.
Environmental and Sex Effects on Bacterial Carriage by Adult House Flies (Musca domestica L.)
Authors: Neupane Saraswoti , White Kotie , Thomson Jessica L. , Zurek Ludek , Nayduch Dana
Published: June 2020

Adult house flies frequent microbe-rich sites such as urban dumpsters and animal facilities, and encounter and ingest bacteria during feeding and reproductive activities. Due to unique nutritional and reproductive needs, male and female flies demonstrate different interactions with microbe-rich substrates and therefore dissemination potential. We investigated culturable aerobic bacteria and coliform abundance in male and female flies (n = 107) collected from urban (restaurant dumpsters) and agricultural (dairy farm) sites. Whole-fly homogenate was aerobically cultured and enumerated on nonselective (tryptic soy agar; culturable bacteria) and selective (violet-red bile agar, VRBA; coliforms) media. Unique morphotypes from VRBA cultures of agricultural flies were identified and tested for susceptibility to 14 antimicrobials. Female flies harbored more bacteria than males and there was a sex by site interaction with sex effects on bacterial abundance at the urban site. Coliform abundance did not differ by sex, site or sex within site. Both male and female flies carried antimicrobial-resistant (AMR) bacteria: 36/38 isolates (95%) were resistant to ≥1 antimicrobial, 33/38 were multidrug-resistant (≥2), and 24/38 isolates were resistant to ≥4 antimicrobials. Our results emphasize the role of house flies in harboring bacteria including AMR strains that pose a risk to human and animal health.
Pituitary Disease in AIP Mutation-Positive Familial Isolated Pituitary Adenoma (FIPA): A Kindred-Based Overview
Authors: Bilbao Garay Ismene , Daly Adrian F. , Egaña Zunzunegi Nerea , Beckers Albert
Published: June 2020

Clinically-relevant pituitary adenomas occur in about 1:1000 of the general population, but only about 5% occur in a known genetic or familial setting. Familial isolated pituitary adenomas (FIPA) are one of the most important inherited settings for pituitary adenomas and the most frequent genetic cause is a germline mutation in the aryl hydrocarbon receptor-interacting protein (AIP) gene. AIP mutations lead to young-onset macroadenomas that are difficult to treat. Most are growth hormone secreting tumors, but all other secretory types can exist and the clinical profile of affected patients is variable. We present an overview of the current understanding of AIP mutation-related pituitary disease and illustrate various key clinical factors using examples from one of the largest AIP mutation-positive FIPA families identified to date, in which six mutation-affected members with pituitary disease have been diagnosed. We highlight various clinically significant features of FIPA and AIP mutations, including issues related to patients with acromegaly, prolactinoma, apoplexy and non-functioning pituitary adenomas. The challenges faced by these AIP mutation-positive patients due to their disease and the long-term outcomes in older patients are discussed. Similarly, the pitfalls encountered due to incomplete penetrance of pituitary adenomas in AIP-mutated kindreds are discussed.
2-(3,4-Dihydroxyphenyl)-4-(2-(4-nitrophenyl)hydrazono)-4H-chromene-3,5,7-triol
Authors: Gianoncelli Alessandra , Ongaro Alberto , Zagotto Giuseppe , Memo Maurizio , Ribaudo Giovanni
Published: June 2020

On the basis of the knowledge from traditional herbal and folk medicine, flavonoids are among the most studied chemical classes of natural compounds for their potential activity as phosphodiesterase 5 (PDE5) inhibitors. We here describe the preparation of a semi-synthetic hydrazone derivative of quercetin, 2-(3,4-dihydroxyphenyl)-4-(2-(4-nitrophenyl)hydrazono)-4H-chromene-3,5,7-triol, that was obtained via a single-step modification of the natural compound. The product was characterized by NMR, mass spectrometry and HPLC. Preliminary molecular modeling studies suggest that this compound could efficiently interact with PDE5.
Harmony Search Optimisation of Dispersed Laminated Composite Plates
Authors: Cakiroglu Celal , Bekdaş Gebrail , Geem Zong
Published: June 2020

One of the major goals in the process of designing structural components is to achieve the highest possible buckling load of the structural component while keeping the cost and weight at a minimum. This paper illustrates the application of the harmony search algorithm to the buckling load maximisation of dispersed laminated composite plates with rectangular geometry. The ply thicknesses and fiber orientation angles of the plies were chosen as the design variables. Besides the commonly used carbon fiber reinforced composites, boron/epoxy and glass/epoxy composite plates were also optimised using the harmony search algorithm. Furthermore, the optimisation algorithm was applied to plates with three different aspect ratios (ratio of the longer side length to the shorter side length of the plate). The buckling loads of the plates with optimised dispersed stacking sequences were compared to the buckling loads of plates with the commonly applied 0°, ±45°, and 90° fiber angle sequence and identical ply thicknesses. For all three aspect ratios and materials in this study, the dispersed stacking sequences performed better than the plates with regular stacking sequences.
Enhancement of Mechanical Properties and Porosity of Concrete Using Steel Slag Coarse Aggregate
Authors: Miah Md Jihad , Patoary Md. Munir Hossain , Paul Suvash Chandra , Babafemi Adewumi John , Panda Biranchi
Published: June 2020

This paper investigates the possibility of utilizing steel slags produced in the steelmaking industry as an alternative to burnt clay brick aggregate (BA) in concrete. Within this context, physical, mechanical (i.e., compressive and splitting tensile strength), length change, and durability (porosity) tests were conducted on concrete made with nine different percentage replacements (0%, 10%, 20%, 30%, 40%, 50%, 60%, 80%, and 100% by volume of BA) of BA by induction of furnace steel slag aggregate (SSA). In addition, the chemical composition of aggregate through X-ray fluorescence (XRF) analysis and microstructural analysis through scanning electron microscopy (SEM) of aggregates and concrete were performed. The experimental results show that the physical and mechanical properties of concrete made with SSA were significantly higher than that of concrete made with BA. The compressive and tensile strength increased by 73% when SSA fully replaced BA. The expansion of concrete made with SSA was a bit higher than the concrete made with BA. Furthermore, a significant lower porosity was observed for concrete made with SSA than BA, which decreased by 40% for 100% SSA concrete than 100% BA concrete. The relation between compressive and tensile strength with the porosity of concrete mixes are in agreement with the relationships presented in the literature. This study demonstrates that SSA can be used as a full replacement of BA, which is economical, conserves the natural aggregate, and is sustainable building material since burning brick produces a lot of CO2.
Modeling and Optimization of Bidirectional Clamping Forces in Drilling of Stacked Aluminum Alloy Plates
Authors: Liu Jintong , Zhao Anan , Wan Piao , Dong Huiyue , Bi Yunbo
Published: June 2020

Interlayer burrs formation during drilling of stacked plates is a common problem in the field of aircraft assembly. Burrs elimination requires extra deburring operations which is time-consuming and costly. An effective way to inhibit interlayer burrs is to reduce the interlayer gap by preloading clamping force. In this paper, based on the theory of plates and shells, a mathematical model of interlayer gap with bidirectional clamping forces was established. The relationship between the upper and lower clamping forces was investigated when the interlayer gap reaches zero. The optimization of the bidirectional clamping forces was performed to reduce the degree and non-uniformity of the deflections of the stacked plates. Then, the finite element simulation was conducted to verify the mathematical model. Finally, drilling experiments were carried out on 2024-T3 aluminum alloy stacked plates based on the dual-machine-based automatic drilling and riveting system. The experimental results show that the optimized bidirectional clamping forces can significantly reduce the burr heights. The work in this paper enables us to understand the effect of bidirectional clamping forces on the interlayer gap and paves the way for the practical application.
Parametric Study on Microwave-Assisted Pyrolysis Combined KOH Activation of Oil Palm Male Flowers Derived Nanoporous Carbons
Authors: Kaewtrakulchai Napat , Faungnawakij Kajornsak , Eiad-Ua Apiluck
Published: June 2020

Oil palm male flowers (PMFs), an abundant agricultural waste from oil palm plantation in Thailand, have been utilized as an alternative precursor to develop nanoporous carbons (NPCs) via microwave-assisted pyrolysis combined potassium hydroxide (KOH) activation. The influences of relevant processing variables, such as activating agent ratio, microwave power, and activation time on the specific pore characteristics, surface morphology, and surface chemistry of PMFs derived nanoporous carbons (PMFCs) have been investigated to explore the optimum preparation condition. The optimum condition under a microwave radiation power of 700 W, activation holding time of 6 min, and activating agent ratio of 2:1 obtained the PMFC with the highest Brunauer–Emmett–Teller (BET) surface area and total pore volume approximately of 991 m2/g and 0.49 cm3/g, composed of a carbon content of 74.56%. Meanwhile, PMFCs have a highly microporous structure of about 71.12%. Moreover, activating agent ratio and microwave radiation power indicated a significant influence on the surface characteristics of PMFCs. This study revealed the potential of oil palm male flowers for the NPCs’ production via microwave-assisted KOH activation with a short operating-time condition.
Deposition of Thin Alumina Films Containing 3D Ordered Network of Nanopores on Porous Substrates
Authors: Tkalčević Marija , Gotić Marijan , Basioli Lovro , Lihter Martina , Dražić Goran , Bernstorff Sigrid , Vuletić Tomislav , Mičetić Maja
Published: June 2020

Self-supporting thin films containing nanopores are very promising materials for use for multiple applications, especially in nanofiltration. Here, we present a method for the production of nanomembranes containing a 3D ordered network of nanopores in an alumina matrix, with a diameter of about 1 nm and a body centered tetragonal structure of the network nodes. The material is produced by the magnetron sputtering deposition of a 3D ordered network of Ge nanowires in an alumina matrix, followed by a specific annealing process resulting in the evaporation of Ge. We demonstrate that the films can be easily grown on commercially available alumina substrates containing larger pores with diameters between 20 and 400 nm. We have determined the minimal film thickness needed to entirely cover the larger pores. We believe that these films have the potential for applications in the fields of filtration, separation and sensing.
High-Performance Multilayer Radiative Cooling Films Designed with Flexible Hybrid Optimization Strategy
Authors: You Peng , Li Xiong , Huang Yijia , Ma Xiaoliang , Pu Mingbo , Guo Yinghui , Luo Xiangang
Published: June 2020

Despite their great potential for energy-saving applications, it is still challenging to design passive radiative cooling (RC) materials with simultaneous high performance and simple structures based on traditional design philosophy. To solve the contradiction between optimization speed and corresponding performance, we present a flexible hybrid optimization strategy based on a genetic algorithm (GA) in conjunction with the transfer matrix method and introducing the calculation of radiative cooling power density in the evaluation function of the GA. As a demonstration, an optimized coating with 1.5-μm-overlapping MgF2 and Si3N4 layers on top of a silver film was numerically designed. Based on a detailed analysis of the material’s electromagnetic properties and cooling performance, this coating achieved a radiative cooling power density of 62 W/m2 and a temperature reduction of 6.8 °C at an ambient temperature of 300 K. Our optimization strategy may have special significance in the design of high-performance RC materials or other multi-spectral engineering materials with simple structures.
An Experimental Study on Bubble Growth in Laponite RD as Thixotropic Yield Material
Authors: Zhang Yiping , Hu Mengxian , Zhou Yongchao
Published: June 2020

The growth and release of the leading major bubble at the tip of a needle in the thixotropic yield material Laponite RD was different from subsequent minor bubbles. The gas injection experiments combined with high-speed camera were conducted. The results showed that the shape of the major bubbles transformed from an inverted carrot shape to an inverted teardrop shape, while the shape of the minor bubbles tended to be elliptical. In addition, the pressure of bubble emergence consisted of hydrostatic pressure, capillary pressure, and cracking pressure. The major and minor bubbles differed only in the cracking pressure. The pressure when the minor bubble detached could be estimated from the lateral hydrostatic pressure. It can be deduced from dimensionless numbers that buoyancy and viscous forces were, respectively, the main driving force and resistance of bubble growth. The yield stress of Laponite RD and inertial force at the initial moment resulted in distinctive behavior of the major bubble. In addition to the viscosity resistance, surface tension, and hydrostatic pressure had a non-negligible influence on minor bubbles and still accounted for 10–20% of the total resistance in the later stage but less than 5% in major bubble growth.
Morphological and Mechanical Characterization of DNA SAMs Combining Nanolithography with AFM and Optical Methods
Authors: Pinto Giulia , Canepa Paolo , Canale Claudio , Canepa Maurizio , Cavalleri Ornella
Published: June 2020

The morphological and mechanical properties of thiolated ssDNA films self-assembled at different ionic strength on flat gold surfaces have been investigated using Atomic Force Microscopy (AFM). AFM nanoshaving experiments, performed in hard tapping mode, allowed selectively removing molecules from micro-sized regions. To image the shaved areas, in addition to the soft contact mode, we explored the use of the Quantitative Imaging (QI) mode. QI is a less perturbative imaging mode that allows obtaining quantitative information on both sample topography and mechanical properties. AFM analysis showed that DNA SAMs assembled at high ionic strength are thicker and less deformable than films prepared at low ionic strength. In the case of thicker films, the difference between film and substrate Young’s moduli could be assessed from the analysis of QI data. The AFM finding of thicker and denser films was confirmed by X-Ray Photoelectron Spectroscopy (XPS) and Spectroscopic Ellipsometry (SE) analysis. SE data allowed detecting the DNA UV absorption on dense monomolecular films. Moreover, feeding the SE analysis with the thickness data obtained by AFM, we could estimate the refractive index of dense DNA films.
Ilmenite Mud Waste as an Additive for Frost Resistance in Sustainable Concrete
Authors: Chyliński Filip , Kuczyński Krzysztof
Published: June 2020

Sustainable development leads to the production of building materials that are safer for the environment. One of the ways to achieve sustainability in materials is the addition of industrial wastes and by-products, especially to concrete. However, the addition of waste to concrete often decreases its durability and the scope of aggression of the environment in which the concrete is used has to be reduced. Making sustainable concrete, which is also durable in more aggressive environments, is rather difficult. This article presents the results of tests performed on concrete containing ilmenite mud waste from the production of titanium dioxide, which was exposed to frost aggression with and without de-icing salts. The results have shown that a sustainable and frost resistant concrete can be made. After 200 freeze–thaw cycles, the compressive strength of the tested concretes decreased by less than 4%. Concretes were highly resistant for scaling and after 112 freeze–thaw cycles in water with de-icing salt, the scaled mass was less than 0.02 kg/m2. The air void distribution has also been analyzed. The results suited the requirements for frost resistance concrete and were similar to those obtained for a reference concrete with fly ash. The examination of the microstructure using scanning electron microscopy (SEM) has not shown any potential risks that might affect the durability of concrete. Particles of waste were thoroughly combined in the binder and some of its constituents seem to be an active part of the cement matrix. Long-term tests of shrinkage (360 days) have not shown any excessive values that would differ from the reference concrete with fly ash. The presented results have shown that sustainable concrete containing ilmenite mud waste from the production of titanium dioxide might also be resistant to frost aggression.
Temperature Dependence of Anisotropy in Ti and Gd Doped NiMnGa-Based Multifunctional Ferromagnetic Shape Memory Alloys
Authors: Łaszcz Amadeusz , Hasiak Mariusz , Kaleta Jerzy
Published: June 2020

The temperature dependence of magnetocrystalline anisotropy was investigated in detail for the polycrystalline Ni50Mn25Ga25, Ni50Mn25Ga20Ti5 and Ni50Mn25Ga20Gd5 ferromagnetic shape memory alloys in the temperature range of 50–400 K. The effective anisotropy constant was estimated from a series of high field magnetization curves based on the fitting procedure according to the law of approach to magnetic saturation. The low temperature martensitic phase was found to have a significantly higher anisotropy energy in comparison to a high temperature austenitic phase, which was observed through a sudden, distinct drop of anisotropy energy. The calculated values of the effective anisotropy constant were comparable to the results published by other authors. Moreover, the strong influence of chemical composition on the first-order phase transition and the second-order ferromagnetic to the paramagnetic transition was revealed. Finally, the strong coupling between the temperature dependence of the coercive field and the temperature dependence of magnetocrystalline anisotropy was also shown and discussed in the present study.
Development of Self-Healing Cement Slurry through the Incorporation of Dual-Encapsulated Polyacrylamide for the Prevention of Water Ingress in Oil Well
Authors: Richhariya G. , Dora D.T.K. , Parmar K.R. , Pant K.K. , Singhal N. , Lal K. , Kundu P.P.
Published: June 2020

In the present work, a novel cross-linked polymer was synthesized though the anionic polymerization of cyanoacrylate with moisture as an initiator, methylene-bis-acrylamide as a cross-linker, and linseed oil as a spacer. Two layers of the synthesized polymer was coated over polyacrylamide for its homogenous impregnation in Class-G cement slurry for the synthesis of cement core. Fourier Transformation Infrared spectroscopy and X-Ray diffraction spectrum of the synthesized polymer and cement core were obtained to investigate the presence of different functional groups and phases. Moreover, the morphologies of the dual-encapsulated polyacrylamide was observed through scanning electron microscopy. Furthermore, the water-absorption capacity of the synthesized dual-encapsulated polyacrylamide in normal and saline conditions were tested. A cement core impregnated with 16% of dosage of dual-encapsulated polyacrylamide possesses an effective self-healing capability during the water-flow test. Moreover, the maximum linear expansion of the cement core was observed to be 26%. Thus, the impregnation of dual-encapsulated polyacrylamide in cement slurry can exhibit a superior self-healing behavior upon water absorption in an oil well.
Influence of CaF2@Al2O3 on Cutting Performance and Wear Mechanism of Al2O3/Ti(C,N)/CaF2@Al2O3 Self-Lubricating Ceramic Tools in Turning
Authors: Zhang Shuai , Xiao Guangchun , Chen Zhaoqiang , Xu Chonghai , Yi Mingdong , Li Qi , Zhang Jingjie
Published: June 2020

This study aimed at improving the cutting performance of a ceramic tool to which were added solid lubricant particles. We prepared the self-lubricating ceramic tool by adding CaF2@Al2O3 instead of CaF2, and the self-lubricating ceramic tool with Al2O3 as matrix phase, Ti(C,N) as reinforcement phase. The properties of the ceramic tool with different contents of CaF2@Al2O3 and CaF2 were studied by turning 40Cr. Compared with the ceramic tool with 10 vol.% CaF2, the main cutting force and the cutting temperature of the ceramic tool with 10 vol.% CaF2@Al2O3 decreased by 67.25% and 38.14% respectively. The wear resistance and machining surface quality of the ceramic tool with CaF2@Al2O3 were better than the ceramic tool to which were directly added CaF2. The optimal content of CaF2@Al2O3 particles was determined to be 10 vol.%. The addition of CaF2@Al2O3 particles effectively reduces the adverse effect of direct addition of CaF2 particles on the ceramic tool, and plays a role in improving the cutting performance of the ceramic tool.
Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products
Authors: Wu Tien-Chiu , Hong Yong-Han , Tsai Yung-Hsiang , Hsieh Shu-Ling , Huang Ren-Han , Kuo Chia-Hung , Huang Chun-Yung
Published: June 2020

Fucoidans possess multiple biological functions including anti-cancer activity. Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans. In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced. The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample. Among SC, SCA, SCH, and SCAH, the chemical compositions varied but structural features were similar. SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells. In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression. SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells. Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer. Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.
Astaxanthin Counteracts Excitotoxicity and Reduces the Ensuing Increases in Calcium Levels and Mitochondrial Reactive Oxygen Species Generation
Authors: García Francisca , Lobos Pedro , Ponce Alejandra , Cataldo Karla , Meza Daniela , Farías Patricio , Estay Carolina , Oyarzun-Ampuero Felipe , Herrera-Molina Rodrigo , Paula-Lima Andrea , Ardiles Álvaro O. , Hidalgo Cecilia , Adasme Tatiana , Muñoz Pablo
Published: June 2020

Astaxanthin (ASX) is a carotenoid pigment with strong antioxidant properties. We have reported previously that ASX protects neurons from the noxious effects of amyloid-β peptide oligomers, which promote excessive mitochondrial reactive oxygen species (mROS) production and induce a sustained increase in cytoplasmic Ca2+ concentration. These properties make ASX a promising therapeutic agent against pathological conditions that entail oxidative and Ca2+ dysregulation. Here, we studied whether ASX protects neurons from N-methyl-D-aspartate (NMDA)-induced excitotoxicity, a noxious process which decreases cellular viability, alters gene expression and promotes excessive mROS production. Incubation of the neuronal cell line SH-SY5Y with NMDA decreased cellular viability and increased mitochondrial superoxide production; pre-incubation with ASX prevented these effects. Additionally, incubation of SH-SY5Y cells with ASX effectively reduced the basal mROS production and prevented hydrogen peroxide-induced cell death. In primary hippocampal neurons, transfected with a genetically encoded cytoplasmic Ca2+ sensor, ASX also prevented the increase in intracellular Ca2+ concentration induced by NMDA. We suggest that, by preventing the noxious mROS and Ca2+ increases that occur under excitotoxic conditions, ASX could be useful as a therapeutic agent in neurodegenerative pathologies that involve alterations in Ca2+ homeostasis and ROS generation.
Targeted Isolation of Rubrolides from the New Zealand Marine Tunicate Synoicum kuranui
Authors: Bracegirdle Joe , Stevenson Luke J. , Page Michael J. , Owen Jeremy G. , Keyzers Robert A.
Published: June 2020

Global natural products social (GNPS) molecular networking is a useful tool to categorize chemical space within samples and streamline the discovery of new natural products. Here, we demonstrate its use in chemically profiling the extract of the marine tunicate Synoicum kuranui, comprised of many previously reported rubrolides, for new chemical entities. Within the rubrolide cluster, two masses that did not correspond to previously reported congeners were detected, and, following MS-guided fractionation, led to the isolation of new methylated rubrolides T (3) and (Z/E)–U (4). Both compounds showed strong growth inhibitory activity against the Gram-positive bacteria Bacillus subtilis, with minimum inhibitory concentration (MIC) values of 0.41 and 0.91 μM, respectively.
The Influence of Printing Orientation on Surface Texture Parameters in Powder Bed Fusion Technology with 316L Steel
Authors: Kozior Tomasz , Bochnia Jerzy
Published: June 2020

Laser technologies for fast prototyping using metal powder-based materials allow for faster production of prototype constructions actually used in the tooling industry. This paper presents the results of measurements on the surface texture of flat samples and the surface texture of a prototype of a reduced-mass lathe chuck, made with the additive technology—powder bed fusion. The paper presents an analysis of the impact of samples’ orientation on the building platform on the surface geometrical texture parameters (two-dimensional roughness profile parameters (Ra, Rz, Rv, and so on) and spatial parameters (Sa, Sz, and so on). The research results showed that the printing orientation has a very large impact on the quality of the surface texture and that it is possible to set digital models on the building platform (parallel—0° to the building platform plane), allowing for manufacturing models with low roughness parameters. This investigation is especially important for the design and 3D printing of microelectromechanical systems (MEMS) models, where surface texture quality and printable resolution are still a large problem.
Piezoelectric Performance of a Symmetrical Ring-Shaped Piezoelectric Energy Harvester Using PZT-5H under a Temperature Gradient
Authors: Zhou Nannan , Li Rongqi , Ao Hongrui , Zhang Chuanbing , Jiang Hongyuan
Published: June 2020

With the rapid development of microelectronics technology, low-power electronic sensors have been widely applied in many fields, such as Internet of Things, aerospace, and so on. In this paper, a symmetrical ring-shaped piezoelectric energy harvester (SR-PEH) is designed to provide energy for the sensor to detect the ambient temperature. The finite element method is used by utilizing software COMSOL 5.4, and the electromechanical coupling model of the piezoelectric cantilever is established. The output performance equations are proposed; the microelectromechanical system (MEMS) integration process of the SR-PEH, circuit, and sensor is stated; and the changing trend of the output power density is explained from an energy perspective. In the logarithmic coordinate system, the results indicate that the output voltage and output power are approximately linear with the temperature when the resistance is constant. In addition, the growth rate of the output voltage and output power decreases with an increase of resistance under the condition of constant temperature. In addition, with an increase of temperature, the growth rate of the output power is faster than that of the output voltage. Furthermore, resistance has a more dramatic effect on the output voltage, whereas temperature has a more significant effect on the output power. More importantly, the comparison with the conventional cantilever-shaped piezoelectric energy harvester (CC-PEH) shows that the SR-PEH can improve the output performance and broaden the frequency band.
Parameters Optimization of Catalytic Tubular Nanomembrane-Based Oxygen Microbubble Generator
Authors: Naeem Sumayyah , Naeem Farah , Zhang Jing , Mujtaba Jawayria , Xu Kailiang , Huang Gaoshan , Solovev Alexander A. , Mei Yongfeng
Published: June 2020

A controllable generation of oxygen gas during the decomposition of hydrogen peroxide by the microreactors made of tubular catalytic nanomembranes has recently attracted considerable attention. Catalytic microtubes play simultaneous roles of the oxygen bubble producing microreactors and oxygen bubble-driven micropumps. An autonomous pumping of peroxide fuel takes place through the microtubes by the recoiling microbubbles. Due to optimal reaction–diffusion processes, gas supersaturation, leading to favorable bubble nucleation conditions, strain-engineered catalytic microtubes with longer length produce oxygen microbubbles at concentrations of hydrogen peroxide in approximately ×1000 lower in comparison to shorter tubes. Dynamic regimes of tubular nanomembrane-based oxygen microbubble generators reveal that this depends on microtubes’ aspect ratio, hydrogen peroxide fuel concentration and fuel compositions. Different dynamic regimes exist, which produce specific bubble frequencies, bubble size and various amounts of oxygen. In this study, the rolled-up Ti/Cr/Pd microtubes integrated on silicon substrate are used to study oxygen evolution in different concentrations of hydrogen peroxide and surfactants. Addition of Sodium dodecyl sulfate (SDS) surfactants leads to a decrease of bubble diameter and an increase of frequencies of bubble recoil. Moreover, an increase of temperature (from 10 to 35 °C) leads to higher frequencies of oxygen bubbles and larger total volumes of produced oxygen.
Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients
Authors: Palma-Lara Icela , Sánchez-Aldana Ana Elena , Jiménez-Hernández Elva , Martínez-Villegas Octavio , Núñez-Enríquez Juan Carlos , Mejía-Aranguré Juan Manuel , Ochoa Sara A. , Xicohtencatl-Cortes Juan , Cruz-Córdova Ariadnna , Zavala-Vega Sergio , García-Jiménez Mariana , Contreras-Ramos Alejandra , Torres-Nava José Refugio , Mora-Ramiro Guillermo , Arellano-Galindo José
Published: June 2020

NOTCH1 and PAX5 participate in the proliferation and differentiation of B and T lymphocytes. Their expression can be modified by activation of NOTCH1, induced by the Epstein–Barr (EBV) viral proteins identified as LMP1 and LMP2. To identify whether PAX5, NOTCH1, and EBV latency genes participate in the oncogenic process of pediatric patients with classical Hodgkin lymphoma (cHL), the present study aimed to identify the variable expression of NOTCH1 among disease subtypes and to assess its effect on PAX5 expression. A total of 41 paraffin-embedded tissues from Mexican pediatric patients with cHL were analyzed. The expression of CD30, CD20, NOTCH1, PAX5, and LMP1 was evaluated by immunohistochemistry and immunofluorescence. EBV detection was performed by in situ hybridization. Out of all cases, 78% (32/41) of the cHL cases were EBV positive. NOTCH1 expression was detected in 78.1% (25/32) of EBV-positive cases, nodular sclerosis being the most frequent subtype (11/25, 44%). In cases where the expression of both genes was identified, double immunofluorescence assays were conducted, finding no colocalization. We found that Reed–Sternberg cells had aberrant expression compared to their cells of origin (B lymphocytes) due to the molecular mechanisms involved in the loss of expression of PAX5 and that the identification of NOTCH1 could be considered as a candidate diagnostic/prognostic marker and a therapeutic target in pediatric cHL.
Arsenic (III) Removal from a High-Concentration Arsenic (III) Solution by Forming Ferric Arsenite on Red Mud Surface
Authors: He Dongdong , Xiong Yuming , Wang Li , Sun Wei , Liu Runqing , Yue Tong
Published: June 2020

Arsenic (As) is considered one of the most serious inorganic pollutants, and the wastewater produced in some smelters contains a high concentration of arsenic. In this paper, we purified the high-concentration arsenic solution with red mud and Fe3+ synergistically. In this system, arsenite anions reacted with Fe(III) ions to form ferric arsenite, which attached on the surface of red mud particles. The generated red mud/Fe1−x(As)x(OH)3 showed a better sedimentation performance than the pure ferric arsenite, which is beneficial to the separation of arsenic from the solution. The red mud not only served as the carrier, but also as the alkaline agent and adsorbent for arsenic treatment. The effects of red mud dosage, dosing order, pH, and molar ratio of Fe/As on arsenic removal were investigated. The efficiency of arsenic removal increased from a pH of 2 to 6 and reached equilibrium at a pH of 7. At the Fe/As molar ratio of 3, the removal efficiency of arsenic ions with an initial concentration of 500 mg/L reached 98%. In addition, the crystal structure, chemical composition, and morphological properties of red mud and arsenic removal residues (red mud/Fe1−x(As)x(OH)3) were characterized by XRD, XPS, X-ray fluorescence (XRF), SEM-EDS, and Raman spectroscopy to study the mechanism of arsenic removal. The results indicated that most of the arsenic was removed from the solution by forming Fe1−x(As)x(OH)3 precipitates on the red mud surface, while the remaining arsenic was adsorbed by the red mud and ferric hydroxide.
Using Whole Rock and Zircon Geochemistry to Assess Porphyry Copper Potential of the Tonggou Copper Deposit, Eastern Tianshan
Authors: Zhang Xue-Bing , Chai Feng-Mei , Chen Chuan , Quan Hong-Yan , Wang Ke-Yong , Li Shun-Da , Wu Shi-Shan
Published: June 2020

Eastern Tianshan hosts a number of porphyry Cu deposits. However, these mainly formed in the Jueluotage Belt, in the middle part of Eastern Tianshan. The Tonggou porphyry Cu mineralization is an exception to this, since it is located in the Bogda Orogenic Belt, north of Eastern Tianshan. We obtained new zircon U-Pb ages, whole-rock geochemical data, zircon Hf isotope data, and zircon trace element compositions. LA-ICP-MS zircon U-Pb dating indicates a crystallization age of 302.2–303.0 Ma for the Tonggou mineralized granodiorite (TMG), which suggests that the Tonggou porphyry Cu mineralization formed in the Late Carboniferous period. εHf (t) data (1.8–14.1) for TMG suggests it was sourced from juvenile crustal melts, mixed with some mantle materials. TMG displays low ΣREE, compatible elements (Ba, Sr, Zr, and Hf), Zr/Hf and Nb/Ta ratios, as well as clearly negative Eu anomalies in whole rocks analyses. In addition, TMG is enriched in P, Hf and Th/U ratios in zircon, and has lower crystallization temperatures (734 to 735 °C) than the Daheyan barren granodiorite (DBG) (753 to 802 °C). Whole rock and zircon geochemical analyses show that the TMG was formed by fractional crystallization to a greater extent than the DBG in the Bogda Orogenic Belt. Moreover, zircon grains of the TMG show high Ce4+/Ce3+ ratios (159–286), which are consistent with related values from large porphyry deposits of the Central Asian Orogenic Belt (CAOB). High Ce4+/Ce3+ ratios reflect oxidizing magmas as a result of fractional crystallization, which indicates that the Tonggou deposit has potential to host a large porphyry Cu deposit.
Stochastic Open-Pit Mine Production Scheduling: A Case Study of an Iron Deposit
Authors: Maleki Mohammad , Jélvez Enrique , Emery Xavier , Morales Nelson
Published: June 2020

Production planning decisions in the mining industry are affected by geological, geometallurgical, economic and operational information. However, the traditional approach to address this problem often relies on simplified models that ignore the variability and uncertainty of these parameters. In this paper, two main sources of uncertainty are combined to obtain multiple simulated block models in an iron ore deposit that include the rock type and seven quantitative variables (grades of Fe, SiO2, S, P and K, magnetic ratio and specific gravity). To assess the effect of integrating these two sources of uncertainty in mine planning decision, stochastic and deterministic production scheduling models are applied based on the simulated block models. The results show the capacity of the stochastic mine planning model to identify and minimize risks, obtaining valuable information in ore content or quality at early stages of the project, and improving decision-making with respect to the deterministic production scheduling. Numerically speaking, the stochastic mine planning model improves 6% expected cumulative discounted cash flow and generates 16% more iron ore than deterministic model.
Synthesis, Cyclooxygenases Inhibition Activities and Interactions with BSA of N-substituted 1H-pyrrolo[3,4-c]pyridine-1,3(2H)-diones Derivatives
Authors: Krzyżak Edward , Szkatuła Dominika , Wiatrak Benita , Gębarowski Tomasz , Marciniak Aleksandra
Published: June 2020

Inhibition of cyclooxygenase is the way of therapeutic activities for anti-inflammatory pharmaceuticals. Serum albumins are the major soluble protein able to bind and transport a variety of exogenous and endogenous ligands, including hydrophobic pharmaceuticals. In this study, a novel N-substituted 1H-pyrrolo[3–c]pyridine-1,3(2H)-diones derivatives were synthesized and biologically evaluated for their inhibitory activity against cyclooxygenases and interactions with BSA. In vitro, COX-1 and COX-2 inhibition assays were performed. Interaction with BSA was studied by fluorescence spectroscopy and circular dichroism measurement. The molecular docking study was conducted to understand the binding interaction of compounds in the active site of cyclooxygenases and BSA. The result of the COX-1 and COX-2 inhibitory studies revealed that all the compounds potentially inhibited COX-1 and COX-2. The IC50 value was found similar to meloxicam. The intrinsic fluorescence of BSA was quenched by tested compounds due to the formation of A/E–BSA complex. The results of the experiment and molecular docking confirmed the main interaction forces between studied compounds and BSA were hydrogen bonding and van der Waals force.