bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Different Rotation Systems on Production and Quality of Black Morel (Morchella importuna)
Authors: Su De-Wei , Song Fei-Fei , Luo Hai-Ling , Lin Hui , Lin Dong-Mei , Liu Peng-Hu , Lin Xing-Sheng , Lin Zhan-Xi , Zhang Li-Li , Lu Guo-Dong
Published: July 2022

Morchella importuna is a highly priced edible and medicinal mushroom. Crop rotation is an important management technique to improve soil health. In this study, the morphological characteristics, chemical composition, and nutritional quality of the M. importuna fruitbody under five different rotation systems (named RSA to RSE) were investigated. The results showed that the fruitbodies of M. importuna in rotation system C (RSC, Rice–Pea–M. importuna rotation) grew best (with the highest yield of 6804.90 kg/hm2) and were of higher quality, which showed significant increases in crude protein (37.32 g/100 g) and decreases in crude fat (4.04 g/100 g), crude fiber (10.06 g/100 g), and total ash (9.32 g/100 g). The heavy metal contents (Pb, Cd, and Hg) in the fruitbodies from all rotation systems were rare or none, which meets the standards of the Chinese Green Food Standard and the maximum limit in foodstuffs of the European Union Standard. In addition, the free amino acid compositions of morel under different rotation systems were analyzed. The ratio of essential amino acids to total amino acids (EAA/TAA) was highest in RSC (37.11%). The tested morels were abundant in umami, sweet, aromatic, and medicinal amino acids (UAA, SAA, AAA and MAA). Combining amino acid score, chemical score, and other nutrition indexes, RSC had the best impact on the yield and quality of morel. Our results demonstrate the feasibility and effectiveness of the cultivation model of rotating “Rice–Vegetables–Fungi” for the production of M. importuna in a way that develops high-quality agriculture.
Determination of the Metabolites and Metabolic Pathways for Three β-Receptor Agonists in Rats Based on LC-MS/MS
Authors: Liang Ying , Wang Lin , Zhang Ruipeng , Pan Jiadi , Wu Wenhong , Huang Yuanyuan , Zhang Zifan , Zhao Renbang
Published: July 2022

This paper developed a universal detection method by high-performance liquid chromatography-tandem mass spectrometry to detect three typical clenbuterols, CLB, SAL, and RAC, and to investigate the metabolism of β-agonists in vivo. The parent ions and daughter ions of the three β-receptor agonist standards and the residues in the muscle, liver, and blood samples of rats were obtained by Total Ions Scan mode. The metabolites produced in different tissues at a specific time were qualitatively and quantitatively analyzed, and the corresponding metabolic pathways were inferred. The results showed that the three β-receptor agonists mainly existed in the form of prototype drugs in rats, with a small amount of clenbuterol methyl compound and albuterol methyl compound. There were significant differences in residual metabolism between different tissues of the same species. In addition, different β-receptor agonists have different absorption and utilization rates in rats.
Shedding Kinetics of Infectious Hematopoietic Necrosis Virus (IHNV) in Juvenile Spring- and Fall-Run Chinook Salmon of the Columbia River Basin
Authors: Hernandez Daniel G. , Kurath Gael
Published: July 2022

This investigation sought to characterize the shedding of infectious hematopoietic necrosis virus (IHNV) in two populations of Columbia River Basin (CRB) Chinook salmon (Oncorhynchus tshawytscha). Juvenile spring- and fall-run Chinook salmon were exposed by immersion to each of three IHN virus strains from the UC, MD, and L subgroups, and then monitored for viral shedding from individual fish for 30 days. Detectable quantities of UC, MD and L IHN virus were shed by a subset of fish from each host population (1–9 out of 10 fish total in each treatment group). Viral shedding kinetics were consistent, with a rapid onset of shedding, peak shedding by 2–3 days, and then a rapid decline to below detectable levels by 7 days’ post-exposure to IHNV. Intraspecies variation was observed as spring Chinook salmon shed more UC virus than fall fish: spring Chinook salmon shed UC virus in greater numbers of fish, with 22-fold higher mean peak shedding magnitude, 33-fold higher mean total virus shed per fish, and 900-fold higher total virus shed per treatment group. The L and MD viruses had comparable shedding at intermediate levels in each host population. All viral shedding occurred well before host mortality began, and shedding magnitude did not correlate with virulence differences. Overall, the greater shedding of UC virus from spring Chinook salmon, combined with low virulence, indicates a uniquely high transmission potential that may explain the predominance of UC viruses in CRB Chinook salmon. This also suggests that spring-run fish may contribute more to the ecology of IHNV in the CRB than fall-run Chinook salmon.
A Lateral-Response Analyzed Model for Piles in Sloping Ground Considering Vertical Resistance Based on Transfer Matrix Method
Authors: Lin Tianjue , Liu Jianxin , Li Xiao
Published: July 2022

In order to analyze the lateral response of piles subjected to combinational loads located at sloping ground, a simplified physical model is proposed and developed based on the Timoshenko beam theory and tri-parameter elastic foundation beam model. In this model, the effects of vertical resistance, shear deformation and initial inclination of pile shafts are taken into account, and the semi-analytical solution to the lateral behavior of pile is obtained by utilizing the transfer matrix approach. Specifically, the relative transfer matrix coefficients in the free, passive, and active segment of the pile are analytically determined through Laplace transformation. In addition, the validation of this model is conducted by comparing with field observations and predictions obtained from other existing methods. Moreover, the influences of initial inclination angle, vertical resistance in passive segments and the vertical load on the lateral response of the pile are also discussed in depth. The results indicate that, although there is a significant reduction effect emerged in both the lateral displacement and bending moment with the increase in the vertical resistance, a growth trend for those responses can be obtained with an increase in the initial inclination angle and vertical load.
Public-Key Cryptography Based on Tropical Circular Matrices
Authors: Huang Huawei , Li Chunhua , Deng Lunzhi
Published: July 2022

Some public-key cryptosystems based on the tropical semiring have been proposed in recent years because of their increased efficiency, since the multiplication is actually an ordinary addition of numbers and there is no ordinary multiplication of numbers in the tropical semiring. However, most of these tropical cryptosystems have security defects because they adopt a public matrix to construct commutative semirings. This paper proposes new public-key cryptosystems based on tropical circular matrices. The security of the cryptosystems relies on the NP-hard problem of solving tropical nonlinear systems of integers. Since the used commutative semiring of circular matrices cannot be expressed by a known matrix, the cryptosystems can resist KU attacks. There is no tropical matrix addition operation in the cryptosystem, and it can resist RM attacks. The new cryptosystems can be considered as a potential post-quantum cryptosystem.
Application of Deep Learning Approach for the Classification of Buildings’ Degradation State in a BIM Methodology
Authors: Rodrigues Fernanda , Cotella Victoria , Rodrigues Hugo , Rocha Eugénio , Freitas Felipe , Matos Raquel
Published: July 2022

Currently, there is extensive research focused on automatic strategies for the segmentation and classification of 3D point clouds, which can accelerate the study of a landmark and integrate it with heterogeneous data and attributes, useful to facilitate the digital management of architectural heritage data. In this work, an automated image-based survey has been exploited a Region- Based Convolutional Neural Network. The training phase has been executed providing examples of images with the anomalies to be detected. At the same time, a laser scanning process was conducted to obtain a point cloud, which acts as a reference for the BIM process. In a final step, a process of projecting information from the images onto the BIM recreates the pathology shapes on the model’s objects, which generates a decision support system for the built environment. The innovation of this research concerns the development of a workflow in which it is possible to automatize the recognition and classification of defects in historical buildings, to finally interpolate this geometric and numerical information with a BIM methodology, obtaining a representation and quantification of the information adapted to the facility management process. The use of innovative techniques such as artificial intelligence algorithms and different plug-ins becomes the main strength of this project.
Evaluation of Salivary Mucin, Amylase, Protein Profile, and Periodontal Parameters among Hypertensive and Diabetic Patients
Authors: Anwar Madiha , Alam Beenish F. , Ali Saqib , Tariq Sahibzadi F. , Aali Khalid , Abrar Eisha , Alotaibi Dalal H. , Alsinaidi Aljoharah A. , Alrahlah Ali , Vohra Fahim
Published: July 2022

Systemic and localized oral disease instigates alterations in salivary composition and content. The objective of the study was to evaluate the levels of salivary amylase, mucin, total protein levels, and periodontal inflammatory parameters in Type 2 Diabetes mellitus (T2DM), hypertensive (HTN) patients, and healthy controls. The study sample was divided into three groups: healthy, T2DM, and HTN. Salivary samples were collected from the included participants and salivary flow rate (SFR) and pH were measured. The salivary levels of amylase, mucin, and total protein concentration were analyzed using an enzyme-linked immunosorbent assay. The effect of anti-hypertensive and hypoglycemic drugs on the salivary flow rate, salivary pH, mucin, amylase, and total protein concentrations was evaluated. The results were analyzed with Chi-squared and analysis of variance to compare the means and standard deviations of variables among the study groups. SFR was significantly (p < 0.01) lower among diabetics (0.78 ± 0.45 mL/min) in comparison to healthy (1.52 ± 0.62 mL/min) and hypertensive (1.07 ± 0.7 mL/min) subjects. PISA values were significantly higher in T2DM (1029 ± 234.6 mm2) and HTN (799.4 ± 155.05 mm2) subjects when compared to controls, indicating a high inflammatory burden of oral cavity caused by these conditions, and showed statistically significant difference between the groups (p-value < 0.001). Mucin levels were significantly higher (p-value < 0.05) in hypertensive patients (4.6 ± 1.17 units) compared to diabetics (3.59 ± 1.03 unit/mL) and healthy (2.26 ± 1.09 units/mL) subjects. Amylase levels were significantly higher among healthy subjects (1.76 ± 0.75 mg/mL) compared to both hypertensive (1.33 ± 1.0 mg/mL) and diabetic (0.88 ± 0.57 mg/mL) patients. Total protein concentration was significantly raised (p-value < 0.001) in diabetics (37.67 ± 3.12 mg/mL) compared to healthy (29.3 ± 3.22 mg/mL) subjects. Significant differences in BOP, CAL, and PPD was observed (p-value < 0.001). Use of antihypertensive and hypoglycemic drugs showed a significant influence on salivary flow rate, protein, mucin, and amylase levels. T2DM and HTN induced irregularities in salivary flow rate, pH, amylase, and mucin levels and showed an increased incidence of moderate to severe periodontitis in patients. UWS levels of SFR, mucin, amylase, and total protein can be used as diagnostic and therapeutic biomarkers in patients with T2DM and HTN with oral disease.
Surface Morphology, Roughness, and Structural Characteristics of Al1−xMgxSb (x = 0 and 0.1) Thin Films Deposited by Chemical Bath Deposition Technique
Authors: Zafar Sadia , Iqbal Muhammad Aamir , Malik Maria , Shahid Wajeehah , Irfan Shaheen , Shabir Muhammad Yasir , Pham Phuong V. , Ryeol Choi Jeong
Published: July 2022

Aluminum antimonide (AlSb), a semiconductor compound, finds its applications in optoelectronics because of its tunable bandgap and promising properties achieved by tailoring suitable dopants. To explore the effects of doping, thin films of pure AlSb and 10% magnesium (Mg)-doped aluminum antimonide (MgAlSb) were synthesized through compound solution deposition on a glass substrate using a low-cost chemical bath deposition (CBD) technique at varying bath temperatures with deposition time intervals ranging from 60 to 180 min. Optical microscopy was used to evaluate the surface roughness and morphology of the synthesized films, revealing the surface roughness and thin film uniformity at different deposition times. The structural characteristics of AlSb and Mg-doped AlSb thin films were further examined using the X-ray diffraction technique, which validated the formation of AlSb and Mg-doped AlSb thin films. This research enables large-scale low-temperature deposition for a variety of conceivable applications in the coatings, materials penetration, energy, and photonic sectors due to the novel properties of this material.
Research on Water Pressure Distribution Characteristics and Lining Safety Evaluation of Deep Shaft in Water-Rich, Large, Fractured Granite Stratum
Authors: Huang Mingli , Yao Xiayi , Tan Zhongsheng , Li Jiabin
Published: July 2022

Building deep shafts in water-rich granite formations with large fissures has difficulties, such as high-water pressure and high construction risks, and is prone to water inrush and shaft flooding. This paper relies on the No. 1 vertical auxiliary shaft project of Gaoligongshan tunnel and obtains the uneven distribution of water pressure on the outside of the lining in the horizontal direction through on-site monitoring data. In order to explain this phenomenon, based on the statistical parameters of actual fractures in the field and the Monte Carlo method, the DFN built in FLAC3D6.0 is used to generate a discrete fracture network, and a dual medium model, considering the distribution of large fractures, is established. The reason for the uneven distribution of water pressure is obtained through research: the large fissures in the surrounding rock make the hydraulic conductivity of each part of the stone body formed after grouting of the surrounding rock different. This results in different osmotic pressures from the hydrostatic pressure outside the grouting ring to the outside of the lining through the grouting ring. Based on the distribution characteristics of water pressure outside the lining, the safety of the lining under non-uniform pressure is studied. The lining safety factor is defined as the ratio of the lining’s normal service limit state load to the actual load. The normal service limit state load is the load when the RFPA software is used to establish a load-structure model to simulate the load when the lining has obvious cracks under the action of external load; the actual load is the monitoring load. The new method and mine design code method are used to evaluate the lining safety and make a comparative analysis. The results show that the new method can effectively calculate the lining safety factor and has a larger safety reserve.
(Bio)Tribocorrosion in Dental Implants: Principles and Techniques of Investigation
Authors: De Stefano Marco , Aliberti Silvana Mirella , Ruggiero Alessandro
Published: July 2022

Tribocorrosion is a current and very discussed theme in tribology and medicine for its impact on industrial applications. Currently, the phenomena are mainly oriented to the biological environment and, in particular, to medical devices such as hip prostheses, dental implants, knee joints, etc. The term tribocorrosion underlines the simultaneous action of wear and corrosion in a tribocouple. It has a non-negligible effect on the total loss of contact materials and the potential failure of the bio-couplings. This overview aims to focus firstly on the basic principles of prosthesis tribocorrosion and subsequently to describe the techniques and the analytical models developed to quantify this phenomenon, reporting the most relevant results achieved in the last 20 years, proposed in chronological order, in order to discuss and to depict the future research developments and tendencies. Despite considerable research efforts, from this investigation come many issues worthy of further investigation, such as how to prevent or minimize tribocorrosion in biological tribopairs, the development of a consolidated protocol for tribological experiments in corrosive environments joined with new biomaterials and composites, the possibility to achieve more and more accurate theoretical models, and how to be able to ensure the success of new implant designs by supporting research and development for the management of implant complications. The above issues certainly constitute a scientific challenge for the next years in the fields of tribology and medicine.
Revalorization of Posidonia oceanica Waste for the Thermochemical Production of Biochar
Authors: Moltó Julia , Montalbán Mercedes G. , Núñez Samuel S. , Jordá Juana D.
Published: July 2022

Every year, many tonnes of Posidonia oceanica are removed from Mediterranean beaches to maintain the quality and pleasure of use of the beaches. Most of this waste ends up in landfills, entailing removal costs. In this work, the Posidonia oceanica material was characterised, and a washing system was developed to obtain biochar. An adequate washing of the starting biomass was shown to play a key role as it led to an over 90% salt content reduction and, therefore, a decrease in conductivity values. The use of biochar as a soil remediator improves soil properties, carbon sequestration, and plant growth. However, not all types of biochars are suitable for this type of application. Therefore, the properties of biochar made from Posidonia oceanica at different temperatures (300, 400, and 500 °C) were studied. All the biochars obtained showed to exceed 10% organic carbon, which is the lower limit to be applied to soils, the maximum percentage having been obtained at 300 °C. In addition, all presented pH values (8.02, 10.32, and 10.38 for the temperatures of 300, 400, and 500 °C, respectively) that were similar to those of other effective biochars for the remediation of acid soils.
Metformin Serves as a Novel Drug Treatment for Arterial Thrombosis: Inhibitory Mechanisms on Collagen-Induced Human Platelet Activation
Authors: Chang Yi , Huang Wei-Chieh , Hsu Chia-Yuan , Hsia Chih-Wei , Jayakumar Thanasekaran , Hsieh Cheng-Ying , Lu Wan-Jung , Chang Chao-Chien
Published: July 2022

Metformin is widely used as first-line medication for type 2 diabetes (T2D), the main disease comorbid with kidney disease, cardiovascular diseases (CVDs), and retinopathy. Platelets are crucial in platelet-dependent arterial thrombosis, which causes CVDs and cerebrovascular diseases. Research indicates that metformin may improve these diseases; metformin reportedly reduced platelet activation in rats. However, no reports have included human platelets. We investigated the mechanisms underlying metformin’s effects on platelet activation by using human platelets and evaluated its in vivo effectiveness in experimental mice. Metformin inhibited platelet aggregation stimulated by collagen but not by arachidonic acid, U46619, or thrombin. Metformin suppressed ATP release, [Ca2+]i mobilization, and P-selectin expression, as well as phospholipase C (PLC)γ2/protein kinase C (PKC), p38 mitogen-activated protein kinase (MAPK), and phosphoinositide 3-kinase (PI3K)/Akt/glycogen synthase kinase-3β (GSK3β) phosphorylation. Metformin did not affect vasodilator-stimulated phosphoprotein (VASP) phosphorylation. In the animal studies, metformin reduced acute pulmonary thromboembolism mortality without increasing bleeding times. These results provide insights into the role and mechanisms of metformin in human platelet activation. Metformin decreased platelet activation by interfering with the PLCγ2/PKC, PI3K/Akt/GSK3β, and p38 MAPK pathways through a VASP-independent mechanism. Metformin demonstrates promise as a new class of antiplatelet agent that can inhibit platelet activation.
Simulation Study of a New Magnetorheological Polishing Fluid Collector Based on Air Seal
Authors: Li Mingchun , Chen Guanci , Zhang Wenbin , Peng Yunfeng , Cao Shuntao , He Jiakuan
Published: July 2022

Inverted magnetorheological (MR) polishing device mainly use a magnetic sealing ring to collect polishing fluid. This collection method wears the wheel surface of the polishing wheel, affects the surface accuracy of the polishing wheel, and introduces machining error. In order to reduce this wear and improve recovery efficiency, a new type of collector using an air seal is proposed in this paper. Furthermore, testing method using six factors and a three-level orthogonal test table is used to study the structural parameters of the new collector. The flow fields affected by the different structural parameters were simulated, and the corresponding collection efficiency was analyzed. The results show that the air nozzle diameter has the greatest impact on the fluctuation value of the collector outlet flow, followed by the airflow velocity and nozzle spacing. Moreover, the structural parameters obtained from the orthogonal test were optimized using the control variable method. The minimum flow fluctuation and maximum flow at the collector outlet can be obtained when the nozzle diameter is 2.5 mm and the nozzle airflow velocity is 31 m/s.
CFD and Energy Loss Model Analysis of High-Speed Centrifugal Pump with Low Specific Speed
Authors: Xue Rong , Lin Xinyi , Zhang Beile , Zhou Hong , Lai Tianwei , Hou Yu
Published: July 2022

High-speed centrifugal pumps with low specific speed have the characteristics of a small flowrate, a high head, and being compact and light weight, making them promising candidates for applications in the thermal management of aerospace and electronic devices. The energy loss in the low specific speed pump is critical and complex due to the large impeller diameter, the narrow and long flow channel, and the small outlet width. In this paper, an analysis method based on an energy loss model and computational fluid dynamics simulations (ELM/CFD) is proposed to analyze the performance of the low specific speed pump with a fully sealed structure. Experiments were carried out under variable water flowrates. The results show that the empirical correlation method failed to accurately predict the performance of high-speed centrifugal pumps, because the bearing clearance leakage and motor channel leakage are ignored. Moreover, the volume loss and hydraulic loss are calculated based on the empirical parameters of commonly used pumps that are different from the high-speed pump with the low specific speed in the complex flow channel structure. The ELM/CFD method calculates various loss power based on the simulation results and can predict the head and efficiency with deviations less than 2% and 5%, respectively. ELM/CFD can accurately analyze the optimization direction of the pump. The hydraulic loss and the volume loss of the impeller are the dominant factors that restrict the pump efficiency under the lower flowrates, while the hydraulic loss of subsequent flow channels becomes important under the larger flowrates.
Special Issue on Advanced Materials and Nanotechnology for Sustainable Energy and Environmental Applications
Authors: Malara Angela , Frontera Patrizia
Published: July 2022

Materials play a very important role in the technological development of a society, greatly impacting people’s daily lives [...]
Repeated Permafrost Formation and Degradation in Boreal Peatland Ecosystems in Relation to Climate Extremes, Fire, Ecological Shifts, and a Geomorphic Legacy
Authors: Jorgenson Mark Torre , Kanevskiy Mikhail , Roland Carl , Hill Kenneth , Schirokauer David , Stehn Sarah , Schroeder Britta , Shur Yuri
Published: July 2022

Permafrost formation and degradation creates a highly patchy mosaic of boreal peatland ecosystems in Alaska driven by climate, fire, and ecological changes. To assess the biophysical factors affecting permafrost dynamics, we monitored permafrost and ecological conditions in central Alaska from 2005 to 2021 by measuring weather, land cover, topography, thaw depths, hydrology, soil properties, soil thermal regimes, and vegetation cover between burned (1990 fire) and unburned terrain. Climate data show large variations among years with occasional, extremely warm–wet summers and cold–snowless winters that affect permafrost stability. Microtopography and thaw depth surveys revealed both permafrost degradation and aggradation. Thaw depths were deeper in post-fire scrub compared to unburned black spruce and increased moderately during the last year, but analysis of historical imagery (1954–2019) revealed no increase in thermokarst rates due to fire. Recent permafrost formation was observed in older bogs due to an extremely cold–snowless winter in 2007. Soil sampling found peat extended to depths of 1.5–2.8 m with basal radiocarbon dates of ~5–7 ka bp, newly accumulating post-thermokarst peat, and evidence of repeated episodes of permafrost formation and degradation. Soil surface temperatures in post-fire scrub bogs were ~1 °C warmer than in undisturbed black spruce bogs, and thermokarst bogs and lakes were 3–5 °C warmer than black spruce bogs. Vegetation showed modest change after fire and large transformations after thermokarst. We conclude that extreme seasonal weather, ecological succession, fire, and a legacy of earlier geomorphic processes all affect the repeated formation and degradation of permafrost, and thus create a highly patchy mosaic of ecotypes resulting from widely varying ecological trajectories within boreal peatland ecosystems.
Ultra-Small and Metabolizable Near-Infrared Au/Gd Nanoclusters for Targeted FL/MRI Imaging and Cancer Theranostics
Authors: Dong Xiawei , Ye Jing , Wang Yihan , Xiong Hongjie , Jiang Hui , Lu Hongbing , Liu Xiaohui , Wang Xuemei
Published: July 2022

Tumor accurate imaging can effectively guide tumor resection and accurate follow-up targeted therapy. The development of imaging-stable, safe, and metabolizable contrast agents is key to accurate tumor imaging. Herein, ultra-small and metabolizable dual-mode imaging probe Au/Gd@FA NCs is rationally engineered by a simple hydrothermal method to achieve accurate FL/MRI imaging of tumors. The probes exhibit ultra-small size (2.5–3.0 nm), near-infrared fluorescence (690 nm), high quantum yield (4.4%), and a better T1 nuclear magnetic signal compared to commercial MRI contrast agents. By modifying the folic acid (FA) molecules, the uptake and targeting of the probes are effectively improved, enabling specific fluorescence imaging of breast cancer. Au/Gd@FA NCs with good biosafety were found to be excreted in the feces after imaging without affecting the normal physiological metabolism of mice. Intracellular reactive oxygen species (ROS) increased significantly after incubation of Au/Gd@FA NCs with tumor cells under 660 nm laser irradiation, indicating that Au/Gd@FA NCs can promote intracellular ROS production and effectively induce cell apoptosis. Thus, metabolizable Au/Gd@FA NCs provide a potential candidate probe for multimodal imaging and tumor diagnosis in clinical basic research. Meanwhile, Au/Gd@FA NCs mediated excessive intracellular production of ROS that could help promote tumor cell death.
Thermally Drawn CNT-Based Hybrid Nanocomposite Fiber for Electrochemical Sensing
Authors: Nishimoto Rino , Sato Yuichi , Wu Jingxuan , Saizaki Tomoki , Kubo Mahiro , Wang Mengyun , Abe Hiroya , Richard Inès , Yoshinobu Tatsuo , Sorin Fabien , Guo Yuanyuan
Published: July 2022

Nowadays, bioelectronic devices are evolving from rigid to flexible materials and substrates, among which thermally-drawn-fiber-based bioelectronics represent promising technologies thanks to their inherent flexibility and seamless integration of multi-functionalities. However, electrochemical sensing within fibers remains a poorly explored area, as it imposes new demands for material properties—both the electrochemical sensitivity and the thermomechanical compatibility with the fiber drawing process. Here, we designed and fabricated microelectrode fibers made of carbon nanotube (CNT)-based hybrid nanocomposites and further evaluated their detailed electrochemical sensing performances. Carbon-black-impregnated polyethylene (CB-CPE) was chosen as the base material, into which CNT was loaded homogeneously in a concentration range of 3.8 to 10 wt%. First, electrical impedance characterization of CNT nanocomposites showed a remarkable decrease of the resistance with the increase in CNT loading ratio, suggesting that CNTs notably increased the effective electrical current pathways inside the composites. In addition, the proof-of-principle performance of fiber-based microelectrodes was characterized for the detection of ferrocenemethanol (FcMeOH) and dopamine (DA), exhibiting an ultra-high sensitivity. Additionally, we further examined the long-term stability of such composite-based electrode in exposure to the aqueous environment, mimicking the in vivo or in vitro settings. Later, we functionalized the surface of the microelectrode fiber with ion-sensitive membranes (ISM) for the selective sensing of Na+ ions. The miniature fiber-based electrochemical sensor developed here holds great potential for standalone point-of-care sensing applications. In the future, taking full advantage of the thermal drawing process, the electrical, optical, chemical, and electrochemical modalities can be all integrated together within a thin strand of fiber. This single fiber can be useful for fundamental multi-mechanistic studies for biological applications and the weaved fibers can be further applied for daily health monitoring as functional textiles.
A Review of the Measurement of the Neurology of Gait in Cognitive Dysfunction or Dementia, Focusing on the Application of fNIRS during Dual-Task Gait Assessment
Authors: Sui Sophia X. , Hendy Ashlee M. , Teo Wei-Peng , Moran Joshua T. , Nuzum Nathan D. , Pasco Julie A.
Published: July 2022

Poor motor function or physical performance is a predictor of cognitive decline. Additionally, slow gait speed is associated with poor cognitive performance, with gait disturbances being a risk factor for dementia. Parallel declines in muscular and cognitive performance (resulting in cognitive frailty) might be driven primarily by muscle deterioration, but bidirectional pathways involving muscle–brain crosstalk through the central and peripheral nervous systems are likely to exist. Following screening, early-stage parallel declines may be manageable and modifiable through simple interventions. Gait–brain relationships in dementia and the underlying mechanisms are not fully understood; therefore, the current authors critically reviewed the literature on the gait–brain relationship and the underlying mechanisms and the feasibility/accuracy of assessment tools in order to identify research gaps. The authors suggest that dual-task gait is involved in concurrent cognitive and motor activities, reflecting how the brain allocates resources when gait is challenged by an additional task and that poor performance on dual-task gait is a predictor of dementia onset. Thus, tools or protocols that allow the identification of subtle disease- or disorder-related changes in gait are highly desirable to improve diagnosis. Functional near-infrared spectroscopy (fNIRS) is a non-invasive, cost-effective, safe, simple, portable, and non-motion-sensitive neuroimaging technique, widely used in studies of clinical populations such as people suffering from Alzheimer’s disease, depression, and other chronic neurological disorders. If fNIRS can help researchers to better understand gait disturbance, then fNIRS could form the basis of a cost-effective means of identifying people at risk of cognitive dysfunction and dementia. The major research gap identified in this review relates to the role of the central/peripheral nervous system when performing dual tasks.
Institutional Trust as a Protective Factor during the COVID-19 Pandemic in China
Authors: Li Shuangshuang , Sun Yijia , Jing Jiaqi , Wang Enna
Published: July 2022

Previous research has demonstrated that institutional trust protects subjective well-being during pandemics. However, the potential mediation mechanisms underlying this linkage remain unclear. This study constructs a mediating model to investigate the effect of institutional trust on subjective well-being and the mediating roles of belief in a just world and fear of COVID-19 in the Chinese context. To this end, we survey a sample of 881 participants. The results show that institutional trust, belief in a just world, fear of COVID-19, and subjective well-being (i.e., life satisfaction, positive affect, and negative affect) are significantly interrelated. The results also indicate a significant impact of institutional trust on life satisfaction, positive affect, and negative affect. Belief in a just world and fear of COVID-19, independently and in sequence, mediate the relationship between institutional trust and subjective well-being.
Natural FRCM and Heritage Buildings: Experimental Approach to Innovative Interventions on “Wall Beams”
Authors: Liberotti Riccardo , Cluni Federico , Faralli Francesco , Gusella Vittorio
Published: July 2022

In this paper, an innovative strengthening system for masonry walls made of externally bonded Fabric-Reinforced Cementitious Matrix (FRCM) is presented. Due to the good mechanical properties and the compatibility with the architectural heritage, the FRCM is an adequate alternative to the use of Fiber-Reinforced Polymer (FRP) composites and other traditional techniques. The proposed system is applied to the strengthening of a classical architectural typology in cultural heritage architecture, which is the “in falso” masonry: a load-bearing wall built over a masonry vault, and hence without a direct load path to the ground. A research program, characterized by an experimental campaign, has been started in order to devise and verify an optimal strengthening system that assures for the masonry wall a structural behavior similar to a “wall beam”, so to prevent progressive collapses when the underlying masonry vault loses its carrier function. In particular, rather than the canonical application, consisting in widespread application to the whole surface of the masonry wall, an innovative intervention made of “Green Tape” of composites has been designed and verified by a specifically designed experimental set-up. The main objective of the research is to propose a reinforcement strategy not detrimental to unmovable artistic assets and tied to the safety and robustness of the architectural heritage.
Frequency of Positive Familial Criteria in Patients with Adenocarcinoma of the Esophageal-Gastric Junction and Stomach: First Prospective Data in a Caucasian Cohort
Authors: Schölzchen Jan , Treese Christoph , Thuss-Patience Peter , Mrózek Alicja , Rau Beate , Seeliger Hendrik , Hartmann Dirk , Estevéz-Schwarz Lope , Siegmund Britta , Horn Denise , Nassir Mani , Daum Severin
Published: July 2022

Objectives: Current prospective studies investigating the frequency of hereditary criteria in a Caucasian population for adenocarcinoma of the esophagogastric junction (AEG) and stomach (GC) are missing. Genetic testing criteria (screening criteria) for hereditary diffuse gastric cancer (HDGC) were updated in 2020, but do not address patients with intestinal histology (familial intestinal gastric cancer FIGC). Thus, we prospectively screened patients residing in Berlin newly diagnosed with AEG or GC for hereditary criteria to gain insights into the frequency of HDGC. Methods: Prospective documentation of familial/clinical parameters in patients residing in Berlin with AEG or GC over three years was conducted. Besides HDGC criteria from 2015 and revised 2020, we also documented patients fulfilling these criteria but with intestinal type gastric cancer (FIGC). Statistical analysis was performed using X2-test. Results: One hundred fifty-three patients were finally included (92 GC; male: 50 (n.s.); 61 AEG; male: 47; p = 0.007). Hereditary criteria for HDGC were detected in 9/92 (9.8%) (2015 criteria) and in 14/92 (15.2%) (2020 criteria) of GC patients (AEG: 2015 criteria 3/61 (4.9%) versus 4/61 according to 2020 criteria (6.5%)). Patients fulfilling hereditary criteria but with intestinal histology (FIGC) increased from 8.7% (2015) to 14.1%, respectively (2020) (AEG: 3.2% (2015) versus 6.6% (2020)). Hereditary criteria including intestinal histology were found in 29.3% (GC) and 13.1% (AEG) (p = 0.03) according to the 2020 criteria. Conclusions: HDGC criteria were found in 15.2% of GC patients according to the 2020 criteria. Percentage increased to 29.3% including patients with intestinal histology among the GC group, and was 13.1% in cases with AEG. These data indicate that family history seems to be of utmost importance in GC to further detect potential hereditary genetic risks. This equally applies for patients with intestinal subtype GC.
FXYD3 Expression Predicts Poor Prognosis in Renal Cell Carcinoma with Immunosuppressive Tumor Microenvironment
Authors: Yonekura Satoru , Ueda Kosuke
Published: July 2022

FXYD3 is a protein-coding gene, belonging to the FXYD protein family associated with Na+/K+-ATPase enzymes and chloride ion channels. Accumulating evidence suggests the biological role of FXYD3 in multiple cancers. However, the prognostic value of FXYD3 expression in clear renal cell carcinoma (KIRC) is unclear. Therefore, we evaluated the clinical data with tumor-infiltrating lymphocytes (TILs) and immunoinhibitory gene expression data using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) dataset (GSE29609). First, the FXYD3 high KIRC patients had distinct clinical characteristics, including age, sex, disease stage, histological grade, and hypoxia-related gene expressions. Next, FXYD3 gene expression was correlated with poor overall survival in both TCGA and GSE29609 cohorts. The ESTIMATE algorithm revealed that higher FXYD3 mRNA levels were associated with increased infiltration of immune cells and tumor purity. Moreover, the FXYD3 high KIRC tissue harbored increased TILs such as B cells, CD8+ T cells, and M1 macrophage, whereas NK cells and neutrophils were decreased. In addition, we showed FXYD3 was co-expressed with several immunoinhibitory genes related to T cell exhaustion such as LGALS9, CTLA4, BTLA, PDCD1, and LAG3. In conclusion, FXYD3 is an unfavorable prognostic biomarker associated with hypoxia, pro-tumor TILs, and T cell exhaustion.
Immunomodulatory Responses of Subcapsular Sinus Floor Lymphatic Endothelial Cells in Tumor-Draining Lymph Nodes
Authors: Sibler Eliane , He Yuliang , Ducoli Luca , Rihs Viviane , Sidler Patrick , Puig-Moreno Claudia , Frey Jasmin , Fujimoto Noriki , Detmar Michael , Dieterich Lothar C.
Published: July 2022

Tumor-draining lymph nodes (LNs), composed of lymphocytes, antigen-presenting cells, and stromal cells, are highly relevant for tumor immunity and the efficacy of immunotherapies. Lymphatic endothelial cells (LECs) represent an important stromal cell type within LNs, and several distinct subsets of LECs that interact with various immune cells and regulate immune responses have been identified. In this study, we used single-cell RNA sequencing (scRNA-seq) to characterize LECs from LNs draining B16F10 melanomas compared to non-tumor-draining LNs. Several upregulated genes with immune-regulatory potential, especially in LECs lining the subcapsular sinus floor (fLECs), were identified and validated. Interestingly, some of these genes, namely, podoplanin, CD200, and BST2, affected the adhesion of macrophages to LN LECs in vitro. Congruently, lymphatic-specific podoplanin deletion led to a decrease in medullary sinus macrophages in tumor-draining LNs in vivo. In summary, our data show that tumor-derived factors induce transcriptional changes in LECs of the draining LNs, especially the fLECs, and that these changes may affect tumor immunity. We also identified a new function of podoplanin, which is expressed on all LECs, in mediating macrophage adhesion to LECs and their correct localization in LN sinuses.
BzATP Activates Satellite Glial Cells and Increases the Excitability of Dorsal Root Ganglia Neurons In Vivo
Authors: Chen Zhiyong , Zhang Chi , Song Xiaodan , Cui Xiang , Liu Jing , Ford Neil C. , He Shaoqiu , Zhu Guangwu , Dong Xinzhong , Hanani Menachem , Guan Yun
Published: July 2022

The purinergic system plays an important role in pain transmission. Recent studies have suggested that activation of P2-purinergic receptors (P2Rs) may be involved in neuron-satellite glial cell (SGC) interactions in the dorsal root ganglia (DRG), but the details remain unclear. In DRG, P2X7R is selectively expressed in SGCs, which closely surround neurons, and is highly sensitive to 3’-O-(4-Benzoyl) benzoyl-ATP (BzATP). Using calcium imaging in intact mice to survey a large number of DRG neurons and SGCs, we examined how intra-ganglionic purinergic signaling initiated by BzATP affects neuronal activities in vivo. We developed GFAP-GCaMP6s and Pirt-GCaMP6s mice to express the genetically encoded calcium indicator GGCaM6s in SGCs and DRG neurons, respectively. The application of BzATP to the ganglion induced concentration-dependent activation of SGCs in GFAP-GCaMP6s mice. In Pirt-GCaMP6s mice, BzATP initially activated more large-size neurons than small-size ones. Both glial and neuronal responses to BzATP were blocked by A438079, a P2X7R-selective antagonist. Moreover, blockers to pannexin1 channels (probenecid) and P2X3R (A317491) also reduced the actions of BzATP, suggesting that P2X7R stimulation may induce the opening of pannexin1 channels, leading to paracrine ATP release, which could further excite neurons by acting on P2X3Rs. Importantly, BzATP increased the responses of small-size DRG neurons and wide-dynamic range spinal neurons to subsequent peripheral stimuli. Our findings suggest that intra-ganglionic purinergic signaling initiated by P2X7R activation could trigger SGC-neuron interaction in vivo and increase DRG neuron excitability.
ZnO Nanowire-Based Piezoelectric Nanogenerator Device Performance Tests
Authors: Serairi Linda , Leprince-Wang Yamin
Published: July 2022

Over the past two decades, the quick development of wireless sensor networks has required the sensor nodes being self-powered. Pushed by this goal, in this work, we demonstrated a ZnO nanowire-array-based piezoelectric nanogenerator (NG) prototype, which can convert mechanical energy into electricity. High-quality single crystalline ZnO nanowires, having an aspect ratio of about 15, grown on gold-coated silicon substrate, were obtained by using a low-cost and low-temperature hydrothermal method. The NG-device fabrication process has been presented in detail, and the NG’s performance has been tested in both compression and vibration modes. Peak power of 1.71 µW was observed across an optimal load resistance of 5 MΩ for the ZnO nanowires-based NG, with an effective area of 0.7 cm2, which was excited in compression mode, at 9 Hz, corresponding to ~38.47 mW/cm3 volume-normalized power output. The measured voltage between the top and bottom electrodes was 5.6 V. In vibration mode, at 500 Hz, the same device showed a potential of 1.4 V peak-to-peak value and an instantaneous power of 0.04 μW, corresponding to an output power density of ~0.9 mW/cm3.
Fluorosubstitution of the Molecular Core in Chiral Esters with Short Terminal Carbon Chains: Influence on Physical Properties
Authors: Deptuch Aleksandra , Piwowarczyk Marcin , Jasiurkowska-Delaporte Małgorzata , Kim Jungeun , Urbańska Magdalena , Skolarczyk Maciej , Jaworska-Gołąb Teresa , Marzec Monika
Published: July 2022

Comparative study of chiral liquid crystalline (S)-(1)-4’-(1-methylheptylcarbonyl) biphenyl-4-yl 4-[4-(2,2,3,3,4,4,4-heptafluorobutoxy)butyl-1-oxy] benzoate (4HH) and (S)-4’-(1-methylheptyloxycarbonyl)biphenyl-4-yl 4-[4-(2,2,3,3,4,4,4-heptafluorobutoxy) butyl-1-oxy]-2,3-difluorobenzoate (4FF) is performed by complementary methods. For 4HH melting of the low-temperature crystal phase and subsequent cold crystallization (from antiferroelectric smectic CA* phase to the high-temperature crystal phase) are reported, crystallization kinetics is examined and a monotropic hexatic SmXA* phase is observed on cooling. For 4FF rich polymorphism in the solid state is investigated mainly by simultaneous X-ray diffraction and differential scanning calorimetry measurements. Influence of fluorosubstitution on structural, electro-optic and dielectric properties of the smectic phases is reported. Unit cell parameters of crystal phases of 4HH and 4FF are determined. The reported results show that the double fluorosubstitution slows down the Goldstone mode and PH phason in the smectic phases and facilitates crystallization.
Costs of Next-Generation Sequencing Assays in Non-Small Cell Lung Cancer: A Micro-Costing Study
Authors: Kumar Srishti , Bennett Alexandria , Campbell Pearl A. , Palidwor Gareth , Lo Bryan , Perkins Theodore J. , Nochaiwong Surapon , Sekhon Harmanjatinder S. , Stewart David J. , Thavorn Kednapa
Published: July 2022

Background: Next-generation sequencing (NGS) of tumor genomes has changed and improved cancer treatment over the past few decades. It can inform clinicians on the optimal therapeutic approach in many of the solid and hematologic cancers, including non-small lung cancer (NSCLC). Our study aimed to determine the costs of NGS assays for NSCLC diagnostics. Methods: We performed a micro-costing study of four NGS assays (Trusight Tumor 170 Kit (Illumina), Oncomine Focus (Thermo Fisher), QIAseq Targeted DNA Custom Panel and QIASeq Targeted RNAscan Custom Panel (Qiagen), and KAPA HyperPlus/SeqCap EZ (Roche)) at the StemCore Laboratories, the Ottawa Hospital, Canada. We used a time-and-motion approach to measure personnel time and a pre-defined questionnaire to collect resource utilization. The unit costs were based on market prices. The cost data were reported in 2019 Canadian dollars. Results: Based on a case throughput of 500 cases per year, the per-sample cost for TruSight Tumor 170 Kit, QIASeq Targeted DNA Custom Panel and QIASeq Targeted RNAscan Custom Panel, Oncomine Focus, and HyperPlus/SeqCap EZ were CAD 1778, CAD 599, CAD 1100 and CAD 1270, respectively. The key cost drivers were library preparation (34–60%) and sequencing (31–51%), followed by data analysis (6–13%) and administrative support (2–7%). Conclusions: Trusight Tumor 170 Kit was the most expensive NGS assay for NSCLC diagnostics; however, an economic evaluation is required to identify the most cost-effective NGS assay. Our study results could help inform decisions to select a robust platform for NSCLC diagnostics from fine needle aspirates, and future economic evaluations of the NGS platforms to guide treatment selections for NSCLC patients.
High Diversity of Medicinal Uses of Thai Legumes (Fabaceae) and Their Potential in Public Herbal Medicine
Authors: Sutjaritjai Natcha , Panyadee Prateep , Phumthum Methee , Inta Angkhana , Balslev Henrik
Published: July 2022

Ethnomedicinal studies have demonstrated that Fabaceae include the highest number of medicinal species of all plant families in Thailand. To evaluate which legumes hold the greatest potential for development of public herbal medicines, we used ethnobotanical indices to screen traditional medicinal species from 152 literature sources published from 1986–2019. The homogeneity of each use category was evaluated using the informant consensus factor (ICF). The popularity of the used species was evaluated by percentage of fidelity level (FL) and use value (UV). A total of 261 species from 98 genera of legumes were recorded for their traditional medicinal uses. The uses included 5387 use reports that covered treatments of 420 disorders. The ethnobotanical indices pointed to 15 species with high use values (e.g., Biancaea sappan (L.) Tod., Cassia fistula L., Senna siamea (Lam.) H.S.Irwin & Barneby). Nine of these species are already used in public herbal medicine. The other six species should be studied in greater depth, especially to identify their bioactive compounds so they can be developed for use in public herbal medicine.