bluuebunny/crossref_metadata_2025 huggingface dataset:
Long Term Retention of Gingival Sealing around Titanium Implants with CaCl2 Hydrothermal Treatment: A Rodent Study
Authors: Ayukawa Yasunori , Oshiro Wakana , Atsuta Ikiru , Furuhashi Akihiro , Kondo Ryosuke , Jinno Yohei , Koyano Kiyoshi
Published: September 2019

We previously reported that CaCl2 hydrothermal-treated (Ca-HT) titanium (Ti) implants induced a tight sealing at the interface between the implant and peri-implant epithelium (PIE) after implantation. However, it is not clear how long this improved epithelium sealing can be maintained. We subsequently investigated whether the positive effect of Ca-HT to promote sealing between the PIE and implant was sustained longer term. Maxillary molars were extracted from rats and replaced with either Ca-HT implants (Ca-HT group), distilled water-HT implants (DW-HT group) or non-treated implants (control group). After 16 weeks, the majority of implants in the Ca-HT group remained at the maxillary with no apical extension of the PIE. Conversely, half the number of control implants was lost following down-growth of the PIE. The effect of Ca-HT on migration and proliferation of rat oral epithelial cells (OECs) was also investigated. In OECs cultured on Ca-HT Ti plates, protein expression in relation to cell migration decreased, and proliferation was higher than other groups. Surface analysis indicated HT enhanced the formation of surface TiO2 layer without altering surface topography. Consequently, Ca-HT of Ti reduced PIE down-growth via tight epithelial attachment to the surface, which may enhance implant capability for a longer time post-implantation.
Preclinical Study in Vivo for New Pharmacological Approaches in Inflammatory Bowel Disease: A Systematic Review of Chronic Model of TNBS-Induced Colitis
Authors: Silva Inês , Pinto Rui , Mateus Vanessa
Published: October 2019

The preclinical studies in vivo provide means of characterizing physiologic interactions when our understanding of such processes is insufficient to allow replacement with in vitro systems and play a pivotal role in the development of a novel therapeutic drug cure. Chemically induced colitis models are relatively easy and rapid to develop. The 2,4,6-trinitrobenzenesulfonic acid (TNBS) colitis model is one of the main models in the experimental studies of inflammatory bowel disease (IBD) since inflammation induced by TNBS mimics several features of Crohn’s disease. This review aims to summarize the existing literature and discuss different protocols for the induction of chronic model of TNBS-induced colitis. We searched MEDLINE via Pubmed platform for studies published through December 2018, using MeSH terms (Crohn Disease.kw) OR (Inflammatory Bowel Diseases.kw) OR (Colitis, Ulcerative.kw) AND (trinitrobenzenesulfonic acid.kw) AND (disease models, animal.kw) AND (mice.all). The inclusion criteria were original articles, preclinical studies in vivo using mice, chronic model of colitis, and TNBS as the inducer of colitis and articles published in English. Chronic TNBS-induced colitis is made with multiple TNBS intrarectal administrations in an average dose of 1.2 mg using a volume lower than 150 μL in 50% ethanol. The strains mostly used are Balb/c and C57BL/6 with 5–6 weeks. To characterize the preclinical model the parameters more used include body weight, stool consistency and morbidity, inflammatory biomarkers like interferon (IFN)-γ, myeloperoxidase (MPO), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10, presence of ulcers, thickness or hyperemia in the colon, and histological evaluation of the inflammation. Experimental chronic colitis is induced by multiple rectal instillations of TNBS increasing doses in ethanol using Balb/c and C57BL/6 mice.
BRAF Mutations and Dysregulation of the MAP Kinase Pathway Associated to Sinonasal Mucosal Melanomas
Authors: Colombino Maria , Paliogiannis Panagiotis , Cossu Antonio , De Re Valli , Miolo Gianmaria , Botti Gerardo , Scognamiglio Giosuè , Ascierto Paolo , Santeufemia Davide , Fraggetta Filippo , Manca Antonella , Sini Maria , Casula Milena , Palomba Grazia , Pisano Marina , Doneddu Valentina , Lissia Amelia , Fedeli Maria , Palmieri Giuseppe
Published: October 2019

Sinonasal mucosal melanoma (SNM) is a rare and aggressive type of melanoma, and because of this, we currently have a limited understanding of its genetic and molecular constitution. The incidence among SNMs of somatic mutations in the genes involved in the main molecular pathways, which have been largely associated with cutaneous melanoma, is not yet fully understood. Through a next-generation sequencing (NGS) approach using a panel of 25 genes involved in melanoma pathogenesis customized by our group, we performed a mutation analysis in a cohort of 25 SNM patients. Results showed that pathogenic mutations were found in more than 60% of SNM cases at a somatic level, with strikingly 32% of them carrying deleterious mutations in the BRAF gene. The identified mutations mostly lack the typical UV signature associated with cutaneous melanomas and showed no significant association with any histopathological parameter. Oncogenic activation of the BRAF-depending pathway, which may induce immune tolerance into the tumour microenvironment (i.e., by increasing the VEGF production) was poorly associated with mutations in genes that have been related to diminished clinical benefit of the treatment with BRAF inhibitors. Screening for mutations in BRAF and other MAPK genes should be included in the routine diagnostic test for a better classification of SNM patients.
An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review
Authors: Diogo Patrícia , F. Faustino M. Amparo , P. M. S. Neves M. Graça , Palma Paulo J. , P. Baptista Isabel , Gonçalves Teresa , Santos João Miguel
Published: September 2019

Apical periodontitis is a biofilm-mediated disease; therefore, an antimicrobial approach is essential to cure or prevent its development. In the quest for efficient strategies to achieve this objective, antimicrobial photodynamic therapy (aPDT) has emerged as an alternative to classical endodontic irrigation solutions and antibiotics. The aim of the present critical review is to summarize the available evidence on photosensitizers (PSs) which has been confirmed in numerous studies from diverse areas combined with several antimicrobial strategies, as well as emerging options in order to optimize their properties and effects that might be translational and useful in the near future in basic endodontic research. Published data notably support the need for continuing the search for an ideal endodontic photosensitizer, that is, one which acts as an excellent antimicrobial agent without causing toxicity to the human host cells or presenting the risk of tooth discoloration. The current literature on experimental studies mainly relies on assessment of mixed disinfection protocols, combining approaches which are already available with aPDT as an adjunct therapy. In this review, several approaches concerning aPDT efficiency are appraised, such as the use of bacteriophages, biopolymers, drug and light delivery systems, efflux pump inhibitors, negative pressure systems, and peptides. The authors also analyzed their combination with other approaches for aPDT improvement, such as sonodynamic therapy. All of the aforementioned techniques have already been tested, and we highlight the biological challenges of each formulation, predicting that the collected information may encourage the development of other effective photoactive materials, in addition to being useful in endodontic basic research. Moreover, special attention is dedicated to studies on detailed conditions, aPDT features with a focus on PS enhancer strategies, and the respective final antimicrobial outcomes. From all the mentioned approaches, the two which are most widely discussed and which show the most promising outcomes for endodontic purposes are drug delivery systems (with strong development in nanoparticles) and PS solubilizers.
An Effective Multiscale Methodology for the Analysis of Marine Flexible Risers
Authors: Edmans B. D. , Pham D. C. , Zhang Z.-Q. , Guo T. F. , Sridhar N. , Stewart G.
Published: September 2019

Life extension is an attractive option for subsea flexible risers nearing the end of their design lives. However, techniques for assessing accumulated fatigue damage in flexible risers are often associated with large uncertainties due to the simplified calculation approaches typically used. One approach to reducing uncertainties is the inclusion of nonlinearities in riser structural response and consistent linking between global and local models. In this article, we present the elements of a numerical multiscale procedure capable of predicting the stresses that lead to fatigue damage in flexible pipes, namely: a nonlinear beam element, a nonlinear section response model and a detailed finite element model; the consistent integration of models developed for different length scales; and finally a validation of the flexible riser large-scale model.
Identification of Mycoses in Developing Countries
Authors: Arastehfar Amir , Wickes Brian L. , Ilkit Macit , Pincus David H. , Daneshnia Farnaz , Pan Weihua , Fang Wenjie , Boekhout Teun
Published: September 2019

Extensive advances in technology offer a vast variety of diagnostic methods that save time and costs, but identification of fungal species causing human infections remains challenging in developing countries. Since the echinocandins, antifungals widely used to treat invasive mycoses, are still unavailable in developing countries where a considerable number of problematic fungal species are present, rapid and reliable identification is of paramount importance. Unaffordability, large footprints, lack of skilled personnel, and high costs associated with maintenance and infrastructure are the main factors precluding the establishment of high-precision technologies that can replace inexpensive yet time-consuming and inaccurate phenotypic methods. In addition, point-of-care lateral flow assay tests are available for the diagnosis of Aspergillus and Cryptococcus and are highly relevant for developing countries. An Aspergillus galactomannan lateral flow assay is also now available. Real-time PCR remains difficult to standardize and is not widespread in countries with limited resources. Isothermal and conventional PCR-based amplification assays may be alternative solutions. The combination of real-time PCR and serological assays can significantly increase diagnostic efficiency. However, this approach is too expensive for medical institutions in developing countries. Further advances in next-generation sequencing and other innovative technologies such as clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic tools may lead to efficient, alternate methods that can be used in point-of-care assays, which may supplement or replace some of the current technologies and improve the diagnostics of fungal infections in developing countries.
The First Two Cases of Candida auris in The Netherlands
Authors: Vogelzang Erik H. , Weersink Annemarie J.L. , van Mansfeld Rosa , Chow Nancy A. , Meis Jacques F. , van Dijk Karin
Published: September 2019

Candida auris is a rapidly emerging multidrug-resistant pathogenic yeast. In recent years, an increasing number of C. auris invasive infections and colonized patients have been reported, and C. auris has been associated with hospital outbreaks worldwide, mainly in intensive care units (ICUs). Here, we describe the first two cases of C. auris in The Netherlands. Both cases were treated in a healthcare facility in India prior to admission. The patients were routinely placed in contact precautions in a single room after admission, which is common practice in The Netherlands for patients with hospitalization outside The Netherlands. No transmission of C. auris was noticed in both hospitals. Routine admission screening both for multidrug-resistant (MDR) bacteria and MDR yeasts should be considered for patients admitted from foreign hospitals or countries with reported C. auris transmission.
The Evaluation of Laser Weldability of the Third-Generation Advanced High Strength Steel
Authors: Pereira António B. , Santos Rafael O. , Carvalho Bruno S. , Butuc Marilena C. , Vincze Gabriela , Moreira Luciano P.
Published: September 2019

To meet the demands of vehicular safety and greenhouse gas emission reduction, the automotive industry is increasingly using advanced high strength steels (AHSS) in the production of the components. With the development of the new generation of AHSS, it is essential to study their behavior towards manufacturing processes used in the automotive industry. For this purpose, the welding capability of newly developed third-generation Gen3 980T steel was investigated using the Nd:YAG (Neodymium:Yittrium Aluminum Garnet) laser-welding with different parameter conditions. The analysis was made by uniaxial tensile tests, micro-hardness, Scanning Electron Microscopy (SEM) and X-Ray Diffraction (XRD). The criteria used to evaluate the quality of the weld were the distance between the fracture and the weld bead and the surface finish. A relationship between the quality of the weld and the energy density was observed, expressed by a partial penetration for values below the optimal, and by irregularities in the weld bead and a high number of spatters for the values above the optimal.
Development of Micropatterns on Curved Surfaces Using Two-Step Ultrasonic Forming
Authors: Park Jong-Han , Park Keun
Published: September 2019

Nanoimprint lithography (NIL) is a micro/nanoscale patterning technology on thermoplastic polymer films, and has been widely used to fabricate functional micro/nanoscale patterns. NIL was also used to develop micro/nanoscale patterns on curved surfaces by employing flexible polymer stamps or micropatterned metal molds with macroscopic curvatures. In this study, two-step ultrasonic forming was used to develop micropatterns on a curved surface out of a flat metal stamp, by connecting ultrasonic imprinting and stretching processes. Ultrasonic imprinting was used to replicate functional micropatterns on a flat polymer film, using a flat ultrasonic horn and micropatterned metal stamps with prism and dot micropatterns. An ultrasonic stretching process was then used to form a curvature on the patterned film using a curved ultrasonic horn and a soft mold insert, to avoid damage to the pre-developed micropatterns. The ultrasonic horn was designed to have three different tip radii, and the resulting forming depth and curvature formation were investigated experimentally. As a result, three different curved surfaces containing two different micropatterns were obtained. The developed curved films containing micropatterns were then evaluated optically, and showed different optical diffusion and illumination characteristics according to the film curvature and micropattern type. These results indicate that the proposed technology can extend the functionality of conventional micropatterned products by imposing appropriate curvatures.
Fabrication and Characterization of Flexible Thermoelectric Generators Using Micromachining and Electroplating Techniques
Authors: Lee Wnag-Lin , Shih Po-Jen , Hsu Cheng-Chih , Dai Ching-Liang
Published: September 2019

This study involves the fabrication and measurement of a flexible thermoelectric generator (FTG) using micromachining and electroplating processes. The area of the FTG is 46 × 17 mm2, and it is composed of 39 thermocouples in series. The thermoelectric materials that are used for the FTG are copper and nickel. The fabrication process involves patterning a silver seed layer on the polymethyl methacrylate (PMMA) substrate using a computer numerical control (CNC) micro-milling machine. Thermoelectric materials, copper and nickel, are deposited on the PMMA substrate using an electroplating process. An epoxy polymer is then coated onto the PMMA substrate. Acetone solution is then used to etch the PMMA substrate and to transfer the thermocouples to the flexible epoxy film. The FTG generates an output voltage (OV) as the thermocouples have a temperature difference (ΔT) between the cold and hot parts. The experiments show that the OV of the FTG is 4.2 mV at ΔT of 5.3 K and the output power is 429 nW at ΔT of 5.3 K. The FTG has a voltage factor of 1 μV/mm2K and a power factor of 19.5 pW/mm2K2. The FTG reaches a curvature of 20 m−1.
Quasi Single Point Calibration Method for High-Speed Measurements of Resistive Sensors
Authors: Botín-Córdoba Jesús A. , Oballe-Peinado Óscar , Sánchez-Durán José A. , Hidalgo-López José A.
Published: September 2019

Direct interface circuits are a simple, inexpensive alternative for the digital conversion of a sensor reading, and in some of these circuits only passive calibration elements are required in order to carry out this conversion. In the case of resistive sensors, the most accurate methods of calibration, namely two-point calibration method (TPCM) and fast calibration methods I and II (FCMs I and II), require two calibration resistors to estimate the value of a sensor. However, although FCMs I and II considerably reduce the time necessary to estimate the value of the sensor, this may still be excessive in certain applications, such as when making repetitive readings of a sensor or readings of a large series of sensors. For these situations, this paper proposes a series of calibration methods that decrease the mean estimation time. Some of the proposed methods (quasi single-point calibration methods) are based on the TPCM, while others (fast quasi single-point calibration methods) make the most of the advantages of FCM. In general, the proposed methods significantly reduce estimation times in exchange for a small increase in errors. To validate the proposal, a circuit with a Xilinx XC3S50AN-4TQG144C FPGA has been designed and resistors in the range (267.56 Ω, 7464.5 Ω) have been measured. For 20 repetitive measurements, the proposed methods achieve time reductions of up to 61% with a relative error increase of only 0.1%.
Dendritic Cells/Macrophages-Targeting Feature of Ebola Glycoprotein and its Potential as Immunological Facilitator for Antiviral Vaccine Approach
Authors: Olukitibi Titus Abiola , Ao Zhujun , Mahmoudi Mona , Kobinger Gary A. , Yao Xiaojian
Published: September 2019

In the prevention of epidemic and pandemic viral infection, the use of the antiviral vaccine has been the most successful biotechnological and biomedical approach. In recent times, vaccine development studies have focused on recruiting and targeting immunogens to dendritic cells (DCs) and macrophages to induce innate and adaptive immune responses. Interestingly, Ebola virus (EBOV) glycoprotein (GP) has a strong binding affinity with DCs and macrophages. Shreds of evidence have also shown that the interaction between EBOV GP with DCs and macrophages leads to massive recruitment of DCs and macrophages capable of regulating innate and adaptive immune responses. Therefore, studies for the development of vaccine can utilize the affinity between EBOV GP and DCs/macrophages as a novel immunological approach to induce both innate and acquired immune responses. In this review, we will discuss the unique features of EBOV GP to target the DC, and its potential to elicit strong immune responses while targeting DCs/macrophages. This review hopes to suggest and stimulate thoughts of developing a stronger and effective DC-targeting vaccine for diverse virus infection using EBOV GP.
Volatile Composition and Sensory Properties of Mead
Authors: Pereira Ana Paula , Mendes-Ferreira Ana , Dias Luís G. , Oliveira José M. , Estevinho Leticia M. , Mendes-Faia Arlete
Published: September 2019

Mead is a traditional beverage that results from the alcoholic fermentation of diluted honey performed by yeasts. Although the process of mead production has been optimized in recent years, studies focused on its sensory properties are still scarce. Therefore, the aim of this work was to analyse the sensory attributes of mead produced with free or immobilized cells of the Saccharomyces cerevisiae strains QA23 and ICV D47, and to establish potential correlations with its volatile composition. In the volatile composition of mead, the effect of yeast condition was more important than the strain. In respect to sensory analysis, the most pleasant aroma descriptors were correlated with mead obtained with free yeast cells, independently of the strain. Both sensory analysis and volatile composition indicates that the most pleasant mead was produced by free yeast cells. Although this study has provided a significant contribution, further research on the sensory quality of mead is still needed.
Opinion: Are Organoids the End of Model Evolution for Studying Host Intestinal Epithelium/Microbe Interactions?
Authors: George Michelle M. , Rahman Mushfiqur , Connors Jessica , Stadnyk Andrew W.
Published: September 2019

In the pursuit to understand intestinal epithelial cell biology in health and disease, researchers have established various model systems, from whole animals (typically rodents) with experimentally induced disease to transformed human carcinomas. The obvious limitation to the ex vivo or in vitro cell systems was enriching, maintaining, and expanding differentiated intestinal epithelial cell types. The popular concession was human and rodent transformed cells of mainly undifferentiated cells, with a few select lines differentiating along the path to becoming goblet cells. Paneth cells, in particular, remained unculturable. The breakthrough came in the last decade with the report of conditions to grow mouse intestinal organoids. Organoids are 3-dimensional ex vivo “mini-organs” of the organ from which the stem cells were derived. Intestinal organoids contain fully differentiated epithelial cells in the same spatial organization as in the native epithelium. The cells are suitably polarized and produce and secrete mucus onto the apical surface. This review introduces intestinal organoids and provide some thoughts on strengths and weaknesses in the application of organoids to further advance our understanding of the intestinal epithelial–microbe relationship.
Numerical Simulation Based Targeting of the Magushan Skarn Cu–Mo Deposit, Middle-Lower Yangtze Metallogenic Belt, China
Authors: Hu Xunyu , Li Xiaohui , Yuan Feng , Ord Alison , Jowitt Simon M. , Li Yue , Dai Wenqiang , Ye Rui , Zhou Taofa
Published: September 2019

The Magushan Cu–Mo deposit is a skarn deposit within the Nanling–Xuancheng mining district of the Middle-Lower Yangtze River Metallogenic Belt (MLYRMB), China. This study presents the results of a new numerical simulation that models the ore-forming processes that generated the Magushan deposit and enables the identification of unexplored areas that have significant exploration potential under areas covered by thick sedimentary sequences that cannot be easily explored using traditional methods. This study outlines the practical value of numerical simulation in determining the processes that operate during mineral deposit formation and how this knowledge can be used to enhance exploration targeting in areas of known mineralization. Our simulation also links multiple subdisciplines such as heat transfer, pressure, fluid flow, chemical reactions, and material migration. Our simulation allows the modeling of the formation and distribution of garnet, a gangue mineral commonly found within skarn deposits (including within the Magushan deposit). The modeled distribution of garnet matches the distribution of known mineralization as well as delineating areas that may well contain high garnet abundances within and around a concealed intrusion, indicating this area should be considered a prospective target during future mineral exploration. Overall, our study indicates that this type of numerical simulation-based approach to prospectivity modeling is both effective and economical and should be considered an additional tool for future mineral exploration to reduce exploration risks when targeting mineralization in areas with thick and unprospective sedimentary cover sequences.
On the Chemical Identification and Classification of Minerals
Authors: Bosi Ferdinando , Biagioni Cristian , Oberti Roberta
Published: September 2019

To univocally identify mineral species on the basis of their formula, the IMA-CNMNC recommends the use of the dominant-valency rule and/or the site-total-charge approach, which can be considered two procedures complementary to each other for mineral identification. In this regard, several worked examples are provided in this study along with some simple suggestions for a more consistent terminology and a straightforward use of mineral formulae. IMA-CNMNC guidelines subordinate the mineral structure to the mineral chemistry in the hierarchical scheme adopted for classification. Indeed, a contradiction appears when we first classify mineral species to form classes (based on their chemistry) and subsequently we group together them to form supergroups (based on their structure topology): To date, more than half of recognized mineral supergroups include species with different anions or anionic complexes. This observation is in contrast to the current use of chemical composition as the distinguishing factor at the highest level of mineral classification.
Comprehensive Recovery Technology for Te, Au, and Ag from a Telluride-Type Refractory Gold Mine
Authors: Yang Wei , Wang Gang , Wang Qian , Dong Ping , Cao Huan , Zhang Kai
Published: September 2019

While extracting gold and silver from telluride-type gold deposits, it is beneficial to develop a comprehensive recovery technology for tellurium. In this paper, we report process mineralogy based on the backward processing technology and the low comprehensive utilization rate of typical telluride-type gold deposits in Xiaoqinling, China. The findings show that tellurium, gold, and silver are the most valuable elements in the ore fissures and gangue minerals and are encapsulated in metallic sulfur ore in the form of altaite, hessite, calaverite, antamokite and natural gold. The flotation method was innovatively applied in this study to comprehensively recover Te, Au and Ag. The results show that when the ore particle size was −0.074 mm (70%), the flotation pulp density was 33%, the pulp pH was 8, and the combined collector (isoamyl xanthate + ethyl thio- carbamate (1:1)) was 120 g/t, in the process involving one rough flotation step, two cleaning flotations and two scavenging flotations as well as a continuous 8 d industrial test, the recovery degree was stable and the average grades of Te, Au, and Ag were 241.61, 90.30, and 92.74 g/t with 95.42%, 97.28%, and 94.65% recovery rates, respectively; thus, excellent recovery degrees were obtained. Compared with the original flotation process, the recovery rates of Te, Au, and Ag were increased by 19.91%, 6.93%, and 5.67%, which boosted the effective enrichment of all valuable elements in the telluride-type gold mine and achieved technological progress.
Synthesis, Biological Activities and Docking Studies of Novel 4-(Arylaminomethyl)benzamide Derivatives as Potential Tyrosine Kinase Inhibitors
Authors: Kalinichenko Elena , Faryna Aliaksandr , Kondrateva Viktoria , Vlasova Alena , Shevchenko Valentina , Melnik Alla , Avdoshko Olga , Belko Alla
Published: September 2019

A number of new compounds containing the 4-(aminomethyl)benzamide fragment as a linker were designed and synthesized, and their biological activities were evaluated as potential anticancer agents. The cytotoxicity activity of the designed compounds was studied in two hematological and five solid cell lines in comparison with the reference drugs. Targeted structures against eight receptor tyrosine kinases including EGFR, HER-2, HER-4, IGF1R, InsR, KDR, PDGFRa, and PDGFRb were investigated. The majority of the compounds showed a potent inhibitory activity against the tested kinases. The analogues 11 and 13 with the (trifluoromethyl)benzene ring in the amide or amine moiety of the molecule were proven to be highly potent against EGFR, with 91% and 92% inhibition at 10 nM, respectively. The docking of synthesized target compounds for nine protein kinases contained in the Protein Data Bank (PDB) database was carried out. The molecular modeling results for analogue 10 showed that the use of the 4-(aminomethyl)benzamide as a flexible linker leads to a favorable overall geometry of the molecule, which allows one to bypass the bulk isoleucine residue and provides the necessary binding to the active center of the T315I-mutant Abl (PDB: 3QRJ).
Water in Mesoporous Confinement: Glass-To-Liquid Transition or Freezing of Molecular Reorientation Dynamics?
Authors: Schranz Wilfried , Soprunyuk Viktor
Published: October 2019

The first mechanical relaxation measurements (f = 400 Hz) of water confined in micro-porous silica were performed more than 40 years ago. The authors reported a so called “capillary transition” (here denoted as P3) of water in the core of the pores and a second one at a lower temperature, which they called the “adsorbate transition” (P1 in present work) related to water near the surface of the pores. The capillary transition was identified with the freezing of water in the centre of the pores. However, even 40 years later, the origin of the adsorbate transition is not yet clear. One study relates it to the liquid-to-glass transition of the supercooled water in the pores, and another study to the freezing of the proton reorientations at the lattice defects. The present work shows the data from extensive dynamic mechanical analysis (DMA) measurements (f = 0.1 Hz–70 Hz) of water confined in mesoporous silica (d = 2.5, 5 and 10 nm), which are in favour of a liquid-to-glass scenario.
New Evaluation of Isoflavone Exposure in the French Population
Authors: Lee Alexandre , Beaubernard Laetitia , Lamothe Valérie , Bennetau-Pelissero Catherine
Published: September 2019

The study relates the present evaluation of exposure to estrogenic isoflavones of French consumers through two approaches: (1) identification of the isoflavone sources in the French food offering, (2) a consumption-survey on premenopausal women. For the foodstuff approach 150 food-items were analysed for genistein and daidzein. Additionally, 12,707 labels of processed-foods from French supermarket websites and a restaurant-supplier website were screened, and 1616 foodstuffs of interest were retained. The sources of phytoestrogens considered were soy, pea, broad bean and lupine. A price analysis was performed. A total of 270 premenopausal women from the French metropolitan territory were interviewed for their global diet habits and soy consumption and perception. In supermarkets, there were significantly less selected foodstuffs containing soy than in restaurant (11.76% vs. 25.71%, p < 0.01). There was significantly more soy in low price-foodstuff in supermarket (p < 0.01). Isoflavone levels ranged from 81 to 123,871 µg per portion of the analyzed soy containing foodstuff. Among the women inquired 46.3% claimed to have soy regularly. Isoflavone intake >45 mg/day is associated to vegan-diet (p < 0.01). In total, 11.9% of soy-consumers had a calculated isoflavone intake >50 mg/day. This dose can lengthen the menstrual cycles. The actual exposure to phytoestrogen is likely to have an effect in a part of the French population.
Intakes of Total, Free, and Naturally Occurring Sugars in the French-Speaking Adult Population of the Province of Québec, Canada: The PREDISE Study
Authors: Bergeron Amélie , Labonté Marie-Ève , Brassard Didier , Bédard Alexandra , Laramée Catherine , Robitaille Julie , Desroches Sophie , Provencher Véronique , Couillard Charles , Vohl Marie-Claude , Lamarche Benoît , Lemieux Simone
Published: September 2019

The objective of this study was to characterize the intakes of different types of sugars in an age- and sex-representative sample of French-speaking adults from five regions of the Province of Québec, Canada, enrolled in the cross-sectional PREDISE (PRÉDicteurs Individuels, Sociaux et Environnementaux) study (n = 1147, 18–65 years old; 50.2% women). Because only total sugar content of foods and beverages is available in the Canadian Nutrient File (CNF) 2015, the initial step of this study was thus to build a database of free and naturally occurring sugars content of each food item and recipe included in the R24W, which is an automated, self-administered, web-based, 24-h dietary recall validated to estimate nutrient intakes in French-speaking adults of the Province of Québec. Total sugars were manually differentiated into free and naturally occurring sugars using a systematic algorithm based on previously published systematic algorithms. The World Health Organization (WHO)’s free sugar definition was used to differentiate total sugars into free and naturally occurring sugars. Dietary intake estimates were assessed using three 24-h dietary recalls completed with the R24W. Mean total, free, and naturally occurring sugar intakes were 116.4 g (19.3% of daily energy intake (%E)), 72.5 g (11.7%E), and 44.0 g (7.5%E), respectively. Over half (57.3%) of the overall sample did not meet the WHO’s recommendation to consume less than 10%E from free sugars. Women had a higher %E from naturally occurring sugars than men and being younger was associated with a greater %E from free sugars. Sugar intakes among French-speaking adults from the Province of Québec were mainly in the form of free sugars, with the majority of the population exceeding the WHO recommendation regarding free sugar intake. This suggests that public health efforts towards reducing free sugar intake in this population are relevant and necessary, considering that overconsumption of free sugars negatively influences health outcomes.
Importance of Zika Virus NS5 Protein for Viral Replication
Authors: Elshahawi Hesham , Syed Hassan Sharifah , Balasubramaniam Vinod
Published: September 2019

Zika virus is the latest addition to an ever-growing list of arboviruses that are causing outbreaks with serious consequences. A few mild cases were recorded between 1960 and 1980 until the first major outbreak in 2007 on Yap Island. This was followed by more severe outbreaks in French Polynesia (2013) and Brazil (2015), which significantly increased both Guillain-Barre syndrome and microcephaly cases. No current vaccines or treatments are available, however, recent studies have taken interest in the NS5 protein which encodes both the viral methyltransferase and RNA-dependent RNA polymerase. This makes it important for viral replication alongside other important functions such as inhibiting the innate immune system thus ensuring virus survival and replication. Structural studies can help design inhibitors, while biochemical studies can help understand the various mechanisms utilized by NS5 thus counteracting them might inhibit or abolish the viral infection. Drug repurposing targeting the NS5 protein has also proven to be an effective tool since hundreds of thousands of compounds can be screened therefore saving time and resources, moreover information on these compounds might already be available especially if they are used to treat other ailments.
Development of an Aryloxazole Derivative as a Brain-Permeable Anti-Glioblastoma Agent
Authors: Shin Seulgi , Lim Sungsu , Song Ji Yeon , Kim Dohee , Choi Min Jeong , Gadhe Changdev G. , Park A Young , Pae Ae Nim , Kim Yun Kyung
Published: September 2019

Glioblastoma drug development has been difficult due to the extremely low blood brain barrier (BBB) penetration of conventional anti-cancer agents. P-glycoprotein, an efflux membrane transporter, is responsible for the poor brain uptake of small and hydrophobic drug substances. To develop brain-penetrable anti-tumor agents, we designed colchicine derivatives containing an aryloxazole moiety, which is known to inhibit P-glycoprotein. Among those tested, an aryloxazole derivative named KIST-G1 showed the strongest anti-glioblastoma cell proliferation activity (IC50 = 3.2 ± 0.8 nM). Compared to colchicine, KIST-G1 showed dramatically increased BBB-permeable properties presenting 51.7 ± 0.5 (10−6 cm/s) parallel artificial membrane permeability assay (PAMPA) permeability and 45.0 ± 6.0% of P-gp inhibition. Aid by the BBB-permeable properties, KIST-G1 (5 mg/kg) suppressed glioblastoma cell growth and migration almost completely in the brain of glioblastoma xenograft models by showing 98.2 ± 0.1% reduced tumor area compared with phosphate buffered saline (PBS)-injected control. In comparison, temozolomide, which is the most widely used drug for glioblastoma, showed only moderate effects. Our results demonstrate the effectiveness of an aryloxazole moiety in targeting brain tumors and suggest KIST-G1 as a potent anti-glioblastoma agent.
Identification of Anther Length QTL and Construction of Chromosome Segment Substitution Lines of Oryza longistaminata
Authors: Ogami Takayuki , Yasui Hideshi , Yoshimura Atsushi , Yamagata Yoshiyuki
Published: September 2019

Life histories and breeding systems strongly affect the genetic diversity of seed plants, but the genetic architectures that promote outcrossing in Oryza longistaminata, a perennial wild species in Africa, are not understood. We conducted a genetic analysis of the anther length of O. longistaminata accession W1508 using advanced backcross quantitative trait locus (QTL) analysis and chromosomal segment substitution lines (CSSLs) in the genetic background of O. sativa Taichung 65 (T65), with simple sequence repeat markers. QTL analysis of the BC3F1 population (n = 100) revealed that four main QTL regions on chromosomes 3, 5, and 6 were associated to anther length. We selected a minimum set of BC3F2 plants for the development of CSSLs to cover as much of the W1508 genome as possible. The additional minor QTLs were suggested in the regional QTL analysis, using 21 to 24 plants in each of the selected BC3F2 population. The main QTLs found on chromosomes 3, 5, and 6 were validated and designated qATL3, qATL5, qATL6.1, and qATL6.2, as novel QTLs identified in O. longistaminata in the mapping populations of 94, 88, 70, and 95 BC3F4 plants. qATL3, qATL5, and qATL6.1 likely contributed to anther length by cell elongation, whereas qATL6.2 likely contributed by cell multiplication. The QTLs were confirmed again in an evaluation of the W1508ILs. In several chromosome segment substitution lines without the four validated QTLs, the anthers were also longer than those of T65, suggesting that other QTLs also increase anther length in W1508. The cloning and diversity analyses of genes conferring anther length QTLs promotes utilization of the genetic resources of wild species, and the understanding of haplotype evolution on the differentiation of annuality and perenniality in the genus Oryza.
Efficient and Precise Micro-Injection Molding of Micro-Structured Polymer Parts Using Micro-Machined Mold Core by WEDM
Authors: Liao Qianghua , Zhou Chaolan , Lu Yanjun , Wu Xiaoyu , Chen Fumin , Lou Yan
Published: September 2019

In this paper, micro-structured polymer parts were efficiently and accurately fabricated by micro-injection molding using a micro-structured mold core machined by wire electrical discharge machining (WEDM). The objective was to realize low-cost mass production and manufacturing of micro-structured polymer products. The regular micro-structured mold core was manufactured by precise WEDM. The micro-structured polymer workpieces were rapidly fabricated by micro-injection molding and the effects of the micro-injection molding process parameters on replication rate and surface roughness of micro-structured polymers were systematically investigated and analyzed. It is shown that the micro-structured polymer can be rapidly and precisely fabricated by the proposed method. The experimental results show the minimum size machining error of the micro-structured mold core and the maximum replication rate of micro-formed polymer were 0.394% and 99.12%, respectively. Meanwhile, the optimal micro-injection molding parameters, namely, jet temperature, melt temperature, injection velocity, holding pressure and holding time were 195 °C, 210 °C, 40 mm/min, 7 Mpa and 5 s, respectively. The surface roughness Ra at the groove bottom and top of the micro-structured polymer workpieces achieved minimum values of 0.805 µm and 0.972 µm, respectively.
Preparation of Immunotherapy Liposomal-Loaded Thermal-Responsive Hydrogel Carrier in the Local Treatment of Breast Cancer
Authors: Tsai Hsieh-Chih , Chou Hsiao-Ying , Chuang Shun-Hao , Lai Juin-Yih , Chen Yi-Shu , Wen Yu-Han , Yu Lu-Yi , Lo Chun-Liang
Published: September 2019

To reduce the side effects of immune drugs and the sustainable release of immune drugs on local parts, we have designed an injectable thermal-sensitive hydrogel containing an imiquimod-loaded liposome system. In the extracellular environment of tumor tissues (pH 6.4), 50% of the drug was released from the carrier, which could be a result of the morphological changes of the liposomal microstructure in the acidic environment. According to the results in animals, the drug-containing liposomes combined with hydrogel can be effectively applied in breast cancer therapy to delay the growth of tumors as well as to dramatically reduce the death rate of mice.
Computational Analysis of Imbalance-Based Irregularity Indices of Boron Nanotubes
Authors: Yang Bin , Munir Mobeen , Rafique Shazia , Ahmad Haseeb , Liu Jia-Bao
Published: October 2019

Molecular topology provides a basis for the correlation of physical as well as chemical properties of a certain molecule. Irregularity indices are used as functions in the statistical analysis of the topological properties of certain molecular graphs and complex networks, and hence help us to correlate properties like enthalpy, heats of vaporization, and boiling points etc. with the molecular structure. In this article we are interested in formulating closed forms of imbalance-based irregularity measures of boron nanotubes. These tubes are known as α-boron nanotube, triangular boron nanotubes, and tri-hexagonal boron nanotubes. We also compare our results graphically and come up with the conclusion that alpha boron tubes are the most irregular with respect to most of the irregularity indices.
The Use of Plant Macrofossils for Paleoenvironmental Reconstructions in Southern European Peatlands
Authors: Souto Martin , Castro Daniel , García-Rodeja Eduardo , Pontevedra-Pombal Xabier
Published: October 2019

The analysis of plant macrofossils in peatland ecosystems has been widely used for the climatic and ecological reconstruction of the Holocene in the high latitudes of the northern hemisphere. By contrast, perhaps associated with rarity of these ecosystems, this proxy has barely been explored for southern Europe. In this work, a compilation and review of existing knowledge on the study of plant macrofossils of peatlands in southern Europe has been carried out, both from a paleoenvironmental perspective and in terms of biodiversity dynamics. Although small in surface area, the peatlands of southern Europe stand out for their diversity (botanical, edaphogenic, morphological, etc.), which has allowed the recovery of a large number of macrofossils from both vascular plants and bryophytes. The southern zone of Europe contains refuge zones with a high plant diversity that have not suffered the intense glaciation of the northern zones, this allows a continuous record since the beginning of the Holocene and the detection of climatic events in lower latitudes, where the ice recession was earlier.
Online and Offline Religion in China: A Protestant WeChat “Alter-Public” through the Bible Handcopying Movement
Authors: Vala Carsten , Huang Jianbo
Published: September 2019

Studies of digital religion frequently take democratic regime settings and developed economic contexts for granted, leaving regime and economic development levels as background factors (Campbell 2013). However, in China, the role of the authoritarian state, restrictions on religion, and rapid social change mean that online and offline religious practices will develop in distinct ways. This article analyzes the 2019 Bible handcopying movement promoted through China’s most popular social media WeChat as a way to commemorate the hundredth anniversary of the publication of China’s most widely used translation of the Bible. Drawing on interviews by and communication with the movement’s founder, the co-authors participated in and collected postings from a 500-member WeChat group from March to August 2019. We argue that while offline handcopying is an innovation in religious practice due to Chinese cultural and historical traditions, the online group constitutes a micro-scale “alter-public” (Chen 2015; Warner 2002), a site for religious discussion, prayer, and devotion that strengthens an “alternative” Protestant identity alongside that of Chinese citizen of the People’s Republic of China.
Leafing Patterns and Drivers across Seasonally Dry Tropical Communities
Authors: Alberton Bruna , da Silva Torres Ricardo , Sanna Freire Silva Thiago , Rocha Humberto , S. B. Moura Magna , Morellato Leonor
Published: September 2019

Investigating the timing of key phenological events across environments with variable seasonality is crucial to understand the drivers of ecosystem dynamics. Leaf production in the tropics is mainly constrained by water and light availability. Identifying the factors regulating leaf phenology patterns allows efficiently forecasting of climate change impacts. We conducted a novel phenological monitoring study across four Neotropical vegetation sites using leaf phenology time series obtained from digital repeated photographs (phenocameras). Seasonality differed among sites, from very seasonally dry climate in the caatinga dry scrubland with an eight-month long dry season to the less restrictive Cerrado vegetation with a six-month dry season. To unravel the main drivers of leaf phenology and understand how they influence seasonal dynamics (represented by the green color channel (Gcc) vegetation index), we applied Generalized Additive Mixed Models (GAMMs) to estimate the growing seasons, using water deficit and day length as covariates. Our results indicated that plant-water relationships are more important in the caatinga, while light (measured as day-length) was more relevant in explaining leafing patterns in Cerrado communities. Leafing behaviors and predictor-response relationships (distinct smooth functions) were more variable at the less seasonal Cerrado sites, suggesting that different life-forms (grasses, herbs, shrubs, and trees) are capable of overcoming drought through specific phenological strategies and associated functional traits, such as deep root systems in trees.
Covariance Analysis of Real-Time Precise GPS Orbit Estimated from Double-Differenced Carrier Phase Observations
Authors: Yu Sunkyoung , Kim Donguk , Song Junesol , Kee Changdon
Published: September 2019

The covariance of real-time global positioning system (GPS) orbits has been drawing attention in various fields such as user integrity, navigation performance improvement, and fault detection. The international global navigation satellite system (GNSS) service (IGS) provides real-time orbit standard deviations without correlations between the axes. However, without correlation information, the provided covariance cannot assure the performance of the orbit product, which would, in turn, causes significant problems in fault detection and user integrity. Therefore, we studied real-time GPS orbit covariance characteristics along various coordinates to effectively provide conservative covariance. To this end, the covariance and precise orbits are estimated by means of an extended Kalman filter using double-differenced carrier phase observations of 61 IGS reference stations. Furthermore, we propose a new method for providing covariance to minimize loss of correlation. The method adopted by the IGS, which neglects correlation, requires 4.5 times the size of the covariance to bind orbit errors. By comparison, our proposed method reduces this size from 4.5 to 1.3 using only one additional parameter. In conclusion, the proposed method effectively provides covariance to users.