bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum
Authors: Mazzi Esquinca Maria Eduarda , Correa Claudia Neves , Marques de Barros Gabriel , Montenegro Horácio , Mantovani de Castro Leandro
Published: March 2023

Sea anemones are sessile invertebrates of the phylum Cnidaria and their survival and evolutive success are highly related to the ability to produce and quickly inoculate venom, with the presence of potent toxins. In this study, a multi-omics approach was applied to characterize the protein composition of the tentacles and mucus of Bunodosoma caissarum, a species of sea anemone from the Brazilian coast. The tentacles transcriptome resulted in 23,444 annotated genes, of which 1% showed similarity with toxins or proteins related to toxin activity. In the proteome analysis, 430 polypeptides were consistently identified: 316 of them were more abundant in the tentacles while 114 were enriched in the mucus. Tentacle proteins were mostly enzymes, followed by DNA- and RNA-associated proteins, while in the mucus most proteins were toxins. In addition, peptidomics allowed the identification of large and small fragments of mature toxins, neuropeptides, and intracellular peptides. In conclusion, integrated omics identified previously unknown or uncharacterized genes in addition to 23 toxin-like proteins of therapeutic potential, improving the understanding of tentacle and mucus composition of sea anemones.
Evaluation of Lipid Extracts from the Marine Fungi Emericellopsis cladophorae and Zalerion maritima as a Source of Anti-Inflammatory, Antioxidant and Antibacterial Compounds
Authors: Abraúl Mariana , Alves Artur , Hilário Sandra , Melo Tânia , Conde Tiago , Domingues Maria Rosário , Rey Felisa
Published: March 2023

Marine environments occupy more than 70% of the earth’s surface, integrating very diverse habitats with specific characteristics. This heterogeneity of environments is reflected in the biochemical composition of the organisms that inhabit them. Marine organisms are a source of bioactive compounds, being increasingly studied due to their health-beneficial properties, such as antioxidant, anti-inflammatory, antibacterial, antiviral, or anticancer. In the last decades, marine fungi have stood out for their potential to produce compounds with therapeutic properties. The objective of this study was to determine the fatty acid profile of isolates from the fungi Emericellopsis cladophorae and Zalerion maritima and assess the anti-inflammatory, antioxidant, and antibacterial potential of their lipid extracts. The analysis of the fatty acid profile, using GC-MS, showed that E. cladophorae and Z. maritima possess high contents of polyunsaturated fatty acids, 50% and 34%, respectively, including the omega-3 fatty acid 18:3 n-3. Emericellopsis cladophorae and Z. maritima lipid extracts showed anti-inflammatory activity expressed by the capacity of their COX-2 inhibition which was 92% and 88% of inhibition at 200 µg lipid mL−1, respectively. Emericellopsis cladophorae lipid extracts showed a high percentage of inhibition of COX -2 activity even at low concentrations of lipids (54% of inhibition using 20 µg lipid mL−1), while a dose-dependent behaviour was observed in Z. maritima. The antioxidant activity assays of total lipid extracts demonstrated that the lipid extract from E. cladophorae did not show antioxidant activity, while Z. maritima gave an IC20 value of 116.6 ± 6.2 µg mL−1 equivalent to 92.1 ± 4.8 µmol Trolox g−1 of lipid extract in the DPPH• assay, and 101.3 ± 14.4 µg mL−1 equivalent to 106.6 ± 14.8 µmol Trolox g−1 of lipid extract in the ABTS•+ assay. The lipid extract of both fungal species did not show antibacterial properties at the concentrations tested. This study is the first step in the biochemical characterization of these marine organisms and demonstrates the bioactive potential of lipid extracts from marine fungi for biotechnological applications.
Retrospective Analysis of Factors Associated with the Treatment Outcomes of Intradiscal Platelet-Rich Plasma-Releasate Injection Therapy for Patients with Discogenic Low Back Pain
Authors: Akeda Koji , Fujiwara Tatsuhiko , Takegami Norihiko , Yamada Junichi , Sudo Akihiro
Published: March 2023

Background and Objectives: Recently, the clinical application of platelet-rich plasma (PRP) has gained popularity for the treatment of degenerative disc diseases. However, the regenerative effects and factors associated with treatment outcomes after intradiscal injection of PRP remain unknown. This study aimed to evaluate time-dependent changes in imaging findings related to intervertebral disc (IVD) degeneration and to identify factors associated with the outcomes of PRP injection therapy. Materials and Methods: A retrospective analysis of a previous randomized clinical trial of intradiscal injection of the releasate isolated from PRP (PRPr) in patients with discogenic low back pain (LBP) was performed. Radiographic parameters (segmental angulation and lumbar lordosis) and MRI phenotypes, including Modic changes, disc bulge, and high-intensity zones (HIZs), were evaluated at baseline and 6 and 12 months post-injection. Treatment outcomes were evaluated based on the degree of LBP and LBP-related disability at 12 months post-injection. Results: A total of 15 patients (mean age: 33.9 ± 9.5 years) were included in this study. Radiographic parameters showed no significant changes after the PRPr injection. There were no remarkable changes in the prevalence or type of MRI phenotype. Treatment outcomes were significantly improved after treatment; however, the number of targeted discs and the presence of posterior HIZs at baseline were significantly but negatively associated with treatment outcomes. Conclusions: Intradiscal injection of PRPr significantly improved LBP and LBP-related disability 12 months post-injection; however, patients with multiple target lesions or posterior HIZs at baseline were significantly associated with poor treatment outcomes.
On the Plasticity and Deformation Mechanisms in Magnesium Crystals
Authors: Molodov Konstantin D. , Al-Samman Talal , Molodov Dmitri A.
Published: March 2023

This work presents an overview of the mechanical response and microstructure evolution of specifically oriented pure magnesium single crystals under plane strain compression at room temperature. Crystals of ‘hard’ orientations compressed along the c-axis exhibited limited room temperature ductility, although pyramidal ⟨c + a⟩ slip was readily activated, fracturing along crystallographic 112¯4 planes as a result of highly localized shear. Profuse 101¯2 extension twinning was the primary mode of incipient deformation in the case of orientations favorably aligned for c-axis extension. In both cases of compression along ⟨112¯0⟩ and ⟨101¯0⟩ directions, 101¯2 extension twins completely converted the starting orientations into twin orientations; the subsequent deformation behavior of the differently oriented crystals, however, was remarkably different. The formation of 101¯2 extension twins could not be prevented by the channel-die constraints when c-axis extension was confined. The presence of high angle grain boundaries and, in particular, 101¯2 twin boundaries was found to be a prerequisite for the activation of 101¯1 contraction twinning by providing nucleation sites for the latter. Prismatic slip was not found to operate at room temperature in the case of starting orientations most favorably aligned for prismatic slip; instead, cooperative 101¯2 extension and 101¯1 contraction twinning was activated. A two-stage work hardening behavior was observed in ‘soft’ Mg crystals aligned for single or coplanar basal slip. The higher work hardening in the second stage was attributed to changes in the microstructure rather than the interaction of primary dislocations with forest dislocations.
A Multi-Label Detection Deep Learning Model with Attention-Guided Image Enhancement for Retinal Images
Authors: Li Zhenwei , Xu Mengying , Yang Xiaoli , Han Yanqi , Wang Jiawen
Published: March 2023

At present, multi-disease fundus image classification tasks still have the problems of small data volumes, uneven distributions, and low classification accuracy. In order to solve the problem of large data demand of deep learning models, a multi-disease fundus image classification ensemble model based on gradient-weighted class activation mapping (Grad-CAM) is proposed. The model uses VGG19 and ResNet50 as the classification networks. Grad-CAM is a data augmentation module used to obtain a network convolutional layer output activation map. Both the augmented and the original data are used as the input of the model to achieve the classification goal. The data augmentation module can guide the model to learn the feature differences of lesions in the fundus and enhance the robustness of the classification model. Model fine tuning and transfer learning are used to improve the accuracy of multiple classifiers. The proposed method is based on the RFMiD (Retinal Fundus Multi-Disease Image Dataset) dataset, and an ablation experiment was performed. Compared with other methods, the accuracy, precision, and recall of this model are 97%, 92%, and 81%, respectively. The resulting activation graph shows the areas of interest for model classification, making it easier to understand the classification network.
The Impact of Early Life Experiences and Gut Microbiota on Neurobehavioral Development in Preterm Infants: A Longitudinal Cohort Study
Authors: Chen Jie , Li Hongfei , Zhao Tingting , Chen Kun , Chen Ming-Hui , Sun Zhe , Xu Wanli , Maas Kendra , Lester Barry M. , Cong Xiaomei S.
Published: March 2023

Objectives: The objective of this study is to investigate the impact of early life experiences and gut microbiota on neurobehavioral development in preterm infants during neonatal intensive care unit (NICU) hospitalization. Methods: Preterm infants were followed from NICU admission until their 28th postnatal day or until discharge. Daily stool samples, painful/stressful experiences, feeding patterns, and other clinical and demographic data were collected. Gut microbiota was profiled using 16S rRNA sequencing, and operational taxonomic units (OTUs) were selected to predict the neurobehaviors. The neurobehavioral development was assessed by the Neonatal Neurobehavioral Scale (NNNS) at 36 to 38 weeks of post-menstrual age (PMA). Fifty-five infants who had NNNS measurements were included in the sparse log-contrast regression analysis. Results: Preterm infants who experienced a high level of pain/stress during the NICU hospitalization had higher NNNS stress/abstinence scores. Eight operational taxonomic units (OTUs) were identified to be associated with NNNS subscales after controlling demographic and clinical features, feeding patterns, and painful/stressful experiences. These OTUs and taxa belonging to seven genera, i.e., Enterobacteriaceae_unclassified, Escherichia-Shigella, Incertae_Sedis, Veillonella, Enterococcus, Clostridium_sensu_stricto_1, and Streptococcus with five belonging to Firmicutes and two belonging to Proteobacteria phylum. The enriched abundance of Enterobacteriaceae_unclassified (OTU17) and Streptococcus (OTU28) were consistently associated with less optimal neurobehavioral outcomes. The other six OTUs were also associated with infant neurobehavioral responses depending on days at NICU stay. Conclusions: This study explored the dynamic impact of specific OTUs on neurobehavioral development in preterm infants after controlling for early life experiences, i.e., acute and chronic pain/stress and feeding in the NICU. The gut microbiota and acute pain/stressful experiences dynamically impact the neurobehavioral development in preterm infants during their NICU hospitalization.
Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF
Authors: Racić Gordana , Vukelić Igor , Kordić Branko , Radić Danka , Lazović Milana , Nešić Ljiljana , Panković Dejana
Published: March 2023

Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecological and biotechnological roles due to their production of a wide range of secondary metabolites, thus regulating plant growth and development or inducing resistance to plant pathogens. In this work we used indigenous Trichoderma strains that were previously isolated from different soil types to determine their tolerance to increased copper and nickel concentrations as well as mechanisms of metal removal. The concentrations of bioavailable metal concentrations were determined after extraction with diethylene-triamine pentaacetate (DTPA)-extractable metals (Cd, Cr, Co, Cu, Pb, Mn, Ni, and Zn) from the soil samples by inductively coupled plasma-optical emission spectrometry (ICP-OES). Two indigenous T. harzianum strains were selected for copper tolerance, and three indigenous T. longibrachiatum strains were selected for nickel tolerance tests. Strains were isolated from the soils with the highest and among the lowest DTPA-extractable metal concentrations to determine whether the adaptation to different concentrations of metals affects the mechanisms of remediation. Mechanisms of metal removal were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence spectroscopy (XRF), non-destructive methods characterized by high measurement speed with little or no need for sample preparation and very low costs. Increased DTPA-extractable metal content for nickel and copper was detected in the soil samples above the target value (TV), and for nickel above the soil remediation intervention values (SRIVs), for total metal concentrations which were previously determined. The SRIV is a threshold of metal concentrations indicating a serious soil contamination, thus confirming the need for soil remediation. The use of FTIR and XRF methods revealed that the presence of both biosorption and accumulation of metals in the Trichoderma cells, providing good bioremediation potential for Ni and Cu.
Candida spp. DNA Extraction in the Age of Molecular Diagnosis
Authors: Codreanu Smaranda Ioana , Ciurea Cristina Nicoleta
Published: March 2023

The standard procedure for the detection of candidemia is blood culture, a method that might require 3–5 days for a positive result. Compared with culturing, molecular diagnosis techniques can provide faster diagnosis. The current paper aimed to present the main strengths and constraints of current molecular techniques for Candida spp. DNA extraction, analyzing their efficiency from a time, price, and ease of usage point of view. A comprehensive search was conducted using the PubMed NIH database for peer-reviewed full-text articles published before October 2022. The studies provided adequate data on the diagnosis of the infection with the Candida spp. DNA extraction is a relevant step in yielding pure qualitative DNA to be amplified in molecular diagnostic techniques. The most used fungal DNA extraction strategies are: mechanical (bead beating, ultrasonication, steel-bullet beating), enzymatic (proteinase K, lysozyme, lyticase), and chemical extraction (formic acid, liquid nitrogen, ammonium chloride). More clinical studies are needed to formulate adequate guidelines for fungal DNA extraction as the current paper highlighted discrepancies in the reported outcome.
Coxiella burnetii Seroprevalence and Associated Risk Factors in Cattle, Sheep, and Goats in Estonia
Authors: Neare Kädi , Tummeleht Lea , Lassen Brian , Viltrop Arvo
Published: March 2023

Q fever, a disease caused by Coxiella burnetii (CB), is an emerging zoonotic health problem. The prevalence data from potential sources are valuable for assessing the risk to human and animal health. To estimate the prevalence of CB antibodies in Estonian ruminants, pooled milk and serum samples from cattle (Bos taurus) and pooled serum samples from sheep (Ovis aries) and goats (Capra hircus) were analyzed. Additionally, bulk tank milk samples (BTM; n = 72) were analyzed for the presence of CB DNA. Questionnaires and herd-level datasets were used to identify the risk factors for exposure using binary logistic regression analysis. The prevalence of CB-positive dairy cattle herds (27.16%) was significantly higher than that in beef cattle herds (6.67%) and sheep flocks (2.35%). No CB antibodies were detected in the goat flocks. CB DNA was found in 11.36% of the BTM samples. The odds of seropositivity were higher in dairy cattle herds, with an increasing number of cattle in the herd, and with location in southwestern, northeastern and northwestern Estonia. Dairy cattle herds had higher odds of testing positive for CB in BTM if the dairy cows were kept loose and lower odds if the herd was located in northwestern Estonia.
The Protective Effects of Goitrin on LPS-Induced Septic Shock in C57BL/6J Mice via Caspase-11 Non-Canonical Inflammasome Inhibition
Authors: Ruan Deqing , Yang Jingyi , Luo Qianfei , Shi Yanhong , Ding Lili , Wang Zhengtao , Wang Rui , Yang Li
Published: March 2023

Septic shock is defined as a subset of sepsis, which is associated with a considerably high mortality risk. The caspase-11 non-canonical inflammasome is sensed and activated by intracellular lipopolysaccharide (LPS) leading to pyroptosis, it plays a critical role in septic shock. However, there are few known drugs that can control caspase-11 non-canonical inflammasome activation. We report here that goitrin, an alkaloid from Radix Isatidis, shows protective effects in LPS-induced septic shock and significant inhibitory effect in caspase-11 non-canonical inflammasome pathway. Male C57BL/6J were injected intraperitoneally with LPS (20 mg/kg) to induce experimental septic shock. The results demonstrated that the survival rates of mice pretreated with goitrin or Toll-like receptor 4 (TLR4) inhibitor TKA-242 increased, and LPS-induced hypothermia and lung damage improved by inhibiting inflammatory response. Elucidating the detailed mechanism, we surprisingly found goitrin is really different from TAK-242, it independent of the TLR4 signal activation, but significantly inhibited the activation of caspase-11 non-canonical inflammasome, including cleaved caspase-11 and N-terminal fragment of gasdermin D (GSDMD-NT). Furthermore, with a nonlethal dose of the TLR3 agonist poly(I:C)-primed and subsequently challenged with LPS to induce caspase-11-mediated lethal septic shock, the efficacy of goitrin had been verified. Those results revealed the effect of goitrin in protective against LPS-induced septic shock via inhibiting caspase-11 non-canonical inflammasome, which provided a new therapeutic strategy for clinical treatment of septic shock.
Evaluating Social Impact of Smart City Technologies and Services: Methods, Challenges, Future Directions
Authors: Hodson Elise , Vainio Teija , Sayún Michel Nader , Tomitsch Martin , Jones Ana , Jalonen Meri , Börütecene Ahmet , Hasan Md Tanvir , Paraschivoiu Irina , Wolff Annika , Yavo-Ayalon Sharon , Yli-Kauhaluoma Sari , Young Gareth W.
Published: March 2023

This study examines motivations, definitions, methods and challenges of evaluating the social impacts of smart city technologies and services. It outlines concepts of social impact assessment and discusses how social impact has been included in smart city evaluation frameworks. Thematic analysis is used to investigate how social impact is addressed in eight smart city projects that prioritise human-centred design across a variety of contexts and development phases, from design research and prototyping to completed and speculative projects. These projects are notable for their emphasis on human, organisational and natural stakeholders; inclusion, participation and empowerment; new methods of citizen engagement; and relationships between sustainability and social impact. At the same time, there are gaps in the evaluation of social impact in both the smart city indexes and the eight projects. Based on our analysis, we contend that more coherent, consistent and analytical approaches are needed to build narratives of change and to comprehend impacts before, during and after smart city projects. We propose criteria for social impact evaluation in smart cities and identify new directions for research. This is of interest for smart city developers, researchers, funders and policymakers establishing protocols and frameworks for evaluation, particularly as smart city concepts and complex technologies evolve in the context of equitable and sustainable development.
Skeletal Muscle Ultrasonography and Muscle Fitness Relationships: Effects of Scanning Plane and Echogenicity Correction
Authors: Voskuil Caleb , Dudar Monique , Zhang Yan , Carr Joshua
Published: March 2023

This study examines the relationships between ultrasonography measurements of skeletal muscle size and echo intensity (EI) with muscle strength and local muscle endurance in a habitually resistance-trained population. Twenty young, healthy participants underwent imaging of the biceps brachii in the sagittal and transverse planes and with the extended field of view (EFOV) technique. Linear regression was used to examine measures of muscle thickness (MT), muscle cross-sectional area (mCSA), EI, and corrected EI (cEI) in each scanning plane for their associations with strength (1RM biceps curl) and local muscle endurance (4x failure @ 50%1RM). The strongest predictor of 1RM strength and local muscle endurance was sagittal MT (adj. R2 = 0.682) and sagittal cEI (adj. R2 = 0.449), respectively. Strength and transverse MT (R2 = 0.661) and the EFOV mCSA (R2 = 0.643) demonstrated a positive relationship. Local muscle endurance and cEI in the transverse plane (R2 = 0.265) and the EFOV scan (R2 = 0.309) demonstrated a negative relationship. No associations were shown with uncorrected EI. While each scanning plane supports the muscle size-strength and echogenicity-endurance relationships, sagittal plane imaging demonstrated the strongest associations with muscle fitness. These findings provide important methodological insights regarding ultrasound imaging and muscle fitness relationships.
Anti-Icing Property of Superhydrophobic Nanostructured Brass via Deposition of Silica Nanoparticles and Nanolaser Treatment
Authors: Hussain Saqib , Muangnapoh Tanyakorn , Traipattanakul Bhawat , Lekmuenwai Milin
Published: March 2023

Ice accumulation on brass surfaces can lead to heat transfer inefficiency, equipment degradation, and potential accidents. To address this issue, superhydrophobic surface technology is utilized. This work aims to develop superhydrophobic nanostructured brass surfaces using the combination of nanolaser ablation and the deposition of silica nanoparticles to achieve the anti-icing property. Four distinct types of brass surfaces namely, the bare surface (BS), the lasered surface (LS), the coated surface (CS), and the coated-lasered surface (CLS) were prepared. The anti-icing performances of the fabricated samples including the effects of the surface structure, the droplet size, and the surface temperature were investigated and evaluated. The results showed that the delayed icing time increased with the increases in the apparent contact angle, the droplet size, and the surface temperature. When the apparent contact angle increased, the contact area between the droplet and the cooling substrate reduced, leading to the longer delayed icing time. With the deposition of silica nanoparticles and nanolaser treatment, CLS achieved the greatest apparent contact angle of 164.5°, resulting in the longest delayed icing time under all experimental conditions. The longest delayed icing time on CLS recorded in this study was 2584 s, which was 575%, 356%, and 27% greater than those on BS, LS, and CS, respectively. The study also revealed that the surface structure played a more crucial role in achieving the anti-icing property when compared to the surface temperature or the droplet size. The shortest delayed icing time on CLS at the lowest surface temperature and at the smallest droplet size was longer than those on BS and LS at all conditions. The results were also discussed in relation to a heat transfer model. The findings of this research can serve as an avenue for advancing knowledge on heat transfer enhancement and energy efficiency.
Rosmarinus officinalis and Mentha piperita Oils Supplementation Enhances Memory in a Rat Model of Scopolamine-Induced Alzheimer’s Disease-like Condition
Authors: Al-Tawarah Nafe M. , Al-dmour Rawand H. , Abu Hajleh Maha N. , Khleifat Khaled M. , Alqaraleh Moath , Al-Saraireh Yousef M. , Jaradat Ahmad Q. , Al-Dujaili Emad A. S.
Published: March 2023

Alzheimer’s disease is regarded as a common neurodegenerative disease that may lead to dementia and the loss of memory. We report here the nootropic and anti-amnesic effects of both peppermint and rosemary oils using a rat model of scopolamine-induced amnesia-like AD. Rats were administered orally with two doses (50 and 100 mg/kg) of each single oil and combined oils. The positive group used donepezil (1 mg/kg). In the therapeutic phase, rats were administered scopolamine (1 mg/kg) through the oral administration of oils. During the nootropic phase, both oils showed a significant (p < 0.05) decrease in radial arm maze latency times, working memory, and reference memory errors compared with the normal group, along with significant (p < 0.05) enhancements of long-term memory during the passive avoidance test. Therapeutic phase results revealed significant enhancements of memory processing compared with the positive groups. In the hippocampus, oils exhibited an elevation of BDNF levels in a dose-dependent manner. Immunohistochemistry findings showed increased hippocampal neurogenesis suppressed by scopolamine in the sub-granular zone, and the anti-amnesic activity of single oil was enhanced when the two oils combined. Gas chromatography–mass spectrometry (GCMS) of the two oils revealed sufficient compounds (1,8-Cineole, α-Pinene, menthol and menthone) with potential efficacy in the memory process and cognitive defects. Our work suggests that both oils could enhance the performance of working and spatial memory, and when combined, more anti-amnesic activity was produced. A potential enhancement of hippocampal growth and neural plasticity was apparent with possible therapeutic activity to boost memory in AD patients.
Fair Competition and Inclusion in Sport: Avoiding the Marginalisation of Intersex and Trans Women Athletes
Authors: Cooper Jonathan
Published: March 2023

Despite the reality of intersex individuals whose biological markers do not necessarily all point towards a traditional binary understanding of either male or female, the vast majority of sports divide competition into categories based on a binary notion of biological sex and develop policies and regulations to police the divide. In so doing, sports governing bodies (SGBs) adopt an imperfect model of biological sex in order to serve their particular purposes, which, typically, will include protecting the fundamental sporting value of fair competition. Yet, one potential consequence of enforcing such an approach would seem to be the exclusion or marginalization of individuals whose biological development does not fit within the binary model, whether due to genetics or through a choice to undergo medical intervention to better represent a chosen gender identity. Any such exclusion or marginalization will inevitably tend to undermine another fundamental value of sport, that of inclusivity. In the context of those with differences of sex development, SGBs appear to be faced with a difficult problem: dealing with a conflict between two fundamental values of sport. Different approaches to this problem have been suggested, with some academics proposing that sports organisations, in general, ought to prioritise fairness above, while others suggest the need to ‘balance’ competing values or even to prioritise inclusion over fairness. However, it is argued that any of these approaches are, in principle, justifiable as any sport should be free to prioritise or balance its own values. What seems more important for any SGB is the need for a rational and transparent justification of regulations that pursue fair competition at the expense of inclusivity. Furthermore, where fair competition and inclusivity are fundamental values, any such justification would seem to demand adherence to some basic norms. First, the regulations should be built on (and articulate) a consistent and principled basis of what ‘fair competition’ means in a particular sport. Second, any regulations should only exclude or restrict participation to the minimum degree necessary to achieve the sense of fair competition so articulated. Third, SGBs should be transparent about what their values are and where their priorities lie so that participants and other stakeholders are able to make an informed choice as to whether they wish to participate in, support, or be associated with a particular sport. With reference to the adoption of the Eligibility Regulations for Athletes with Differences of Sex Development (the ‘DSD Regulations’) by the International Association of Athletic Federations (IAAF), this article seeks to evaluate whether the approach of the IAAF satisfied these basic requirements.
Influence of Off-Axis Noncanonical Vortex on the Dynamics of Energy Flux
Authors: Zhao Xinying , Liang Huijian , Wu Gaofeng , Pang Xiaoyan
Published: March 2023

In this article, we analyze the impact of the off-axis noncanonical vortex on the dynamics of 3D energy flux. The results reveal that the properties of energy flux are significantly influenced by the anisotropy parameter σc of the off-axis noncanonical vortex. It is demonstrated that by adjusting the anisotropy parameter, we can control the transverse energy flux, from the position of the transverse energy tornado and the distribution of the transverse energy strength to the rotation direction of the transverse energy flux as the beam propagates. The interesting phenomenon, the reversed energy flux, is also closely related to the anisotropy parameter. The position and size of the reversed energy flux region, as well as the production of two energy tornadoes with opposite rotating directions, resembling a Chinese “Taiji” pattern, can be controlled by varying the anisotropy parameter. This result will potentially provide a new freedom for tailoring the 3D optical field and be useful in optical manipulation and processing.
High-Power, Narrow-Linewidth, Continuous-Wave, Thulium-Doped Fiber Laser Based on MOPA
Authors: Guan Biao , Yan Fengping , Han Wenguo , Qin Qi , Yang Dandan , Li Ting , Yu Chenhao , Wang Xiangdong , Kumamoto Kazuo , Suo Yuping
Published: March 2023

A high-power, narrow-linewidth, continuous-wave, thulium-doped fiber laser (TDFL) based on a master-oscillator power-amplifier (MOPA) was experimentally demonstrated. The main oscillator (seed source) yielded 0.64 W of narrow-linewidth laser output at a central wavelength of 1940.32 nm and a 3 dB spectral bandwidth of 0.05 nm. The output narrow-linewidth laser from the main oscillator was amplified by two-stage, cladding-pumped, thulium-doped, all-fiber amplifiers. The main amplifier yielded 26 W of narrow-linewidth laser at a central wavelength of 1940.33 nm. The slope efficiency of the main amplifier was approximately 55.6%. Significant residual pumping light component in the output laser was not observed. During the amplification process, no stimulated Brillouin scattering (SBS) effect, strong amplified spontaneous emission (ASE) effect, and parasitic lasers were observed at the reverse monitoring end. Moreover, the output power was only limited by the incident pump power and the output power had a good stability in a 50 min monitoring period.
Optical Diagnostics in Herpetic Keratitis
Authors: Di Staso Federico , Rullo Daria , Di Pippo Mariachiara , Di Staso Silvio , Abdolrahimzadeh Solmaz
Published: March 2023

One of the leading global causes of vision impairment due to anterior segment disease is herpes simplex keratitis (HSK). The routine clinical method in the diagnosis of HSK is examination of the eye using slit lamp biomicroscopy; nevertheless, this is a subjective examination and can potentially lead to an erroneous diagnosis. Optical devices such as in vivo confocal microscopy and anterior segment optical coherence tomography are among the additional diagnostic tools that provide a valuable resource in the diagnosis and management of the condition. In research settings, these technologies have already enhanced our understanding of the microscopic causes of numerous common in vivo observations. This review aims to highlight the multiple emerging clinical and research applications for optical imaging devices in HSK.
Effect of Surface Nonlinearity Distribution on Second Harmonic Generation under Tightly Focused Beams
Authors: Scherbak Sergey , Reshetov Ilya , Kan Gennadiy , Lipovskii Andrey
Published: March 2023

A thorough integral formulation describing the second harmonic generation under tightly focused linearly and radially polarized beams for an arbitrary distribution of the nonlinear susceptibility over the surface of isotropic media was presented. We numerically simulated effects caused by the straight edge of the nonlinear region. In particular, we demonstrated that the second harmonic radiation in the normal direction, which is forbidden in common cases of highly symmetrical nonlinear surfaces, is allowed in the presence of the edge. This is provided by χ(2)xzx-component of the second-order susceptibility.
Lithography Alignment Techniques Based on Moiré Fringe
Authors: Jiang Wenbo , Wang Huaran , Xie Wenda , Qu Zhefei
Published: March 2023

In Moiré fringe lithography alignment technology, alignment is realized by monitoring the grating interference fringe image in real-time. The technique exhibits excellent sensitivity to displacement changes and is not easily affected by the gap changes between the mask and silicon wafer. Therefore, this technique is widely used in conventional proximity and contact lithography and new-generation micro- and nanolithography systems. The rapid development of semiconductor and integrated circuit industries, as well as the increasing requirements for the resolution of various nanodevices and systems, have posed new challenges in Moiré fringe lithography alignment technology, which are mainly reflected in alignment accuracy, alignment range, and scheme complexity. In this study, the development history, alignment principle, and overall process of Moiré fringe lithography alignment technology are reviewed; the main factors affecting alignment accuracy are analyzed, and corresponding optimization schemes are provided; and finally, the development trend and research focus of Moiré fringe lithography alignment technology are predicted from the marking structure, alignment scheme, and algorithm processing.
Triple-Wavelength Thulium-Doped Fiber Random Laser Based on Random Fiber Grating
Authors: Zhou Lewen , Hu Yaozong , Zheng Wenlong , Xu Pengbai , Gao Zhensen , Dong Xinyong
Published: March 2023

We demonstrate a triple-wavelength thulium-doped fiber random laser using a 10 cm long random fiber grating to provide random distributed feedback and a superimposed fiber Bragg grating as the wavelength-selective mirror. The random fiber grating inscribed in single-mode fibers using a femtosecond laser provides strong random distributed feedback that avoids the use of long distance fibers and leads to a relatively low threshold power. Triple-wavelength random laser output at wavelengths of 1943.6, 1945.0 and 1946.3 nm was achieved with a relatively low threshold power of 2.01 W, a slope efficiency of 7.86% and a maximum output power of 151.8 mW when it was pumped using a 793 nm laser diode. The 3 dB linewidth was less than 0.1 nm and the optical signal-to-noise ratio was up to 45.6 dB. Good wavelength stability was achieved, which was attributed to the narrow band and stable reflection of the superimposed fiber Bragg grating. The time-domain characteristics of the laser output were also measured and analyzed, and some random self-pulsing caused by relaxation oscillations were observed.
Strategy for Attacking the Key Parameters of Electro-Optic Self-Feedback Phase Encryption System
Authors: Zhang Lihong , Huang Wenkun , Tang Bin , Luo Ying , An Yuehua , Wang Yuncai , Qin Yuwen , Gao Zhensen
Published: March 2023

In this paper, we propose a method for cracking the key parameters of an electro-optic self-feedback temporal optical phase encryption system and experimentally demonstrate the feasibility of the scheme. By scanning a tunable dispersion compensation (TDC) module at the receiver, the time delay signature (TDS) of an encrypted signal can be exposed, making it possible to extract other key parameters of the system and reconstruct a decryption setup. The TDS characteristics for three typical modulation formats are investigated, revealing that while such an encryption system is secure against power detection attack, there is a risk of TDS leakage. The findings can guide the design of advanced optical encryption schemes with TDS suppression for security enhancement.
Analysis of Compounding and Broadband Extinction Properties of Novel Bioaerosols
Authors: Chen Xi , Hu Yihua , Gu Youlin , Wang Xinyu , Wang Peng
Published: March 2023

Artificially prepared microbial spores have excellent electromagnetic attenuation properties due to their special composition and structure. At present, studies on the optical properties of microbial spores have mainly focused on those with a single band or a single germplasm, which has limitations and cannot reveal the optical properties comprehensively. In this paper, 3 kinds of laboratory-prepared microbial spores were selected for compounding, and the spectral reflectivities of single-germplasm biospores and compound biospores were measured in the wavebands of 0.25–2.4 and 3–15 μm. The complex refractive indices (CRIs) were calculated in combination with the Kramers–Kronig (K-K) algorithm. Relying on the smoke box broadband test system, the transmittance of single-germplasm bioaerosols and compound bioaerosols from the ultraviolet (UV) band to the far-infrared (FIR) band was measured, and the mass extinction coefficients were calculated. The results indicate that the trend of the complex refractive indices of the compound spores is consistent with that of the single-germplasm spores with a larger particle size. For the single-germplasm bioaerosols, the lowest transmittance values were 2.21, 5.70 and 6.27% in the visible (VIS), near-infrared (NIR) and middle-infrared (FIR) bands, and the mass extinction coefficients reached 1.15, 0.87 and 0.84 m2/g, respectively. When AO and BB spores were compounded at 4:1, the extinction performance of the bioaerosols somewhat improved in all wavebands. These results can help to comprehensively analyze the optical properties of bioaerosols and provide ideas for the development of new extinction materials.
Association of High and Low Molecular Weight Glutenin Subunits with Gluten Strength in Tetraploid Durum Wheat (Triticum turgidum spp. Durum L.)
Authors: Al-Khayri Jameel , Alshegaihi Rana , Mahgoub ELsayed , Mansour Elsayed , Atallah Osama , Sattar Muhammad , Al-Mssallem Muneera , Alessa Fatima , Aldaej Mohammed , Hassanin Abdallah
Published: March 2023

The gluten strength and the composition of high- and low-molecular-weight glutenin subunits (HMWGSs and LMWGSs) of fifty-one durum wheat genotypes were evaluated using sodium dodecyl sulfate (SDS) sedimentation testing and SDS polyacrylamide gel electrophoresis (SDS-PAGE). This study examined the allelic variability and the composition of HMWGSs and LMWGSs in T. durum wheat genotypes. SDS-PAGE was proven to be a successful method for identifying HMWGS and LMWGS alleles and their importance in determining the dough quality. The evaluated durum wheat genotypes with HMWGS alleles 7+8, 7+9, 13+16, and 17+18 were highly correlated with improved dough strength. The genotypes containing the LMW-2 allele displayed stronger gluten than those with the LMW-1 allele. The comparative in silico analysis indicated that Glu-A1, Glu-B1, and Glu-B3 possessed a typical primary structure. The study also revealed that the lower content of glutamine, proline, glycine, and tyrosineand the higher content of serine and valine in the Glu-A1 and Glu-B1 glutenin subunits, and the higher cysteine residues in Glu-B1 and lower arginine, isoleucine, and leucine in the Glu-B3 glutenin, are associated with the suitability of durum wheat for pasta making and the suitability of bread wheat with good bread-making quality. The phylogeny analysis reported that both Glu-B1 and Glu-B3 had a closer evolutionary relationship in bread and durum wheat, while the Glu-A1 was highly distinct. The results of the current research may help breeders to manage the quality of durum wheat genotypes by exploiting the allelic variation in glutenin. Computational analysis showed the presence of higher proportions of glutamine, glycine, proline, serine, and tyrosine than the other residues in both HMWGSs and LMWGSs. Thus, durum wheat genotype selection according to the presence of a few protein components effectively distinguishes the strongest from the weakest types of gluten.
Phylogeny and Systematics of Sassafras (Lauraceae), an Interesting Genus with Disjunct Distributions in Eastern North America and East Asia
Authors: Zhang Yunyan , Zhou Jingbo , Tng David Y. P. , Wang Shuang , Wang Ying , Peng Ye , Liu Hong , Wang Zhongsheng
Published: March 2023

The Lauraceae is a family of the order Laurales, with 2500–3000 species comprising 50 genera, mainly distributed in tropical and subtropical evergreen broad-leaved forests. Up to two decades ago, the systematic classification of the Lauraceae was based on floral morphology, but molecular phylogenetic approaches have made tremendous advances in elucidating tribe- and genus-level relationships within the family in recent decades. Our review focused on the phylogeny and systematics of Sassafras, a genus of three species with highly disjunct distributions in eastern North America and East Asia, whose tribe affiliation within the Lauraceae has long been controversial. By synthesizing information on the floral biology and molecular phylogeny of Sassafras, this review aimed to explore the position of Sassafras within the Lauraceae, and to provide suggestions and implications for future phylogenetic studies. Our synthesis highlighted Sassafras as a transitional type between Cinnamomeae and Laureae with a closer genetic relationship with Cinnamomeae, as revealed by molecular phylogenetic evidence, while it shares many similar characteristics with Laureae in morphology. We therefore discovered that several molecular and morphological methods should be concurrently considered to illuminate the phylogeny and systematics of Sassafras in Lauraceae.
Numerical Simulation of Pre-Reduction for a New Process of Acid Production from Phosphogypsum by Gas Sulfur Reduction
Authors: Chen Yanxin , Fan Xuyang , Zhao Bo , Zhang Leilei
Published: March 2023

The gas sulfur reduction of phosphogypsum in the acid co-production of sulfoaluminate cement clinker is a new process for treating phosphogypsum. The reduction furnace of this system was studied and analyzed by combining computational fluid dynamics (CFD) and experimental validation. The effects of n(CaSO4)/n(S2), particle residence time, and kiln tail flue gas temperature on the performance of the reduction furnace were obtained. A second-order response model based on the response surface methodology was developed using a three-factor Box–Behnken design (BBD). The results show that the comparison error between the simulation and test data of the reduction furnace is acceptable. The above three conditions arranged in order of significance in terms of their effect on the performance of the reduction furnace is n(CaSO4)/n(S2) > particle residence time > kiln tail gas temperature. Finally, by optimizing the response surface model, the predicted optimal operation parameter combination is n(CaSO4)/n(S2) = 3.04, with the particle residence time and flue gas temperature at the kiln end given as 8.90 s and 1265.39 K, respectively.
A Novel Dead Time Design Method for Full-Bridge LLC Resonant Converters with SiC Semiconductors
Authors: Wang Longxiang , Luo Wenguang , Wang Yuewu , Lan Hongli
Published: March 2023

As third-generation semiconductors become commercial, SiC semiconductors are gradually becoming more widely used in LLC resonant converters. The efficiency of the LLC resonant converter is improved by employing soft switching. However, when designing LLC resonant converters, semiconductors are usually regarded as ideal devices, and their turn-on and turn-off times are neglected. Furthermore, the method of designing the dead time relies on engineering experience and lacks precise theoretical foundations. In order to overcome the shortcomings of the current empirical method and to improve the generality and practicality of the dead time design method, a novel method for calculating the dead time of full-bridge LLC converters is proposed through theoretical research based on the operating principle of full-bridge LLC converters and the conditions for implementing soft switching. The method takes into account the switching characteristics of semiconductors and the on-state delay time of their body diodes, stray inductance, drive circuits, and errors arising from the first harmonic approximation (FHA) and improves the accuracy of the dead time calculation. It can implement good soft switching with full-bridge LLC converters, reduce switching losses, and improve system efficiency. Finally, the simulation experiment and the 2 kW experimental prototype are built to verify the effectiveness of the proposed method.
The Beneficial Effect of Salicornia herbacea Extract and Isorhamnetin-3-O-Glucoside on Obesity
Authors: Lee Ji Hwan , Lee Sanghyun , Park Jun Yeon , Park Il-Ho , Kang Ki Sung , Shin Myoung-Sook
Published: March 2023

Salicornia herbacea is a halophyte indigenous to marine coastal areas and salt fields and has been used as a traditional remedy for diarrhea, abdominal pain, constipation, and indigestion. Its component isorhamnetin-3-O-glucoside (IR3G) may have antioxidant, anti-inflammatory, and anti-adipogenic properties. In the present study, we aimed to investigate the anti-obesity effect of S. herbacea extract and IR3G on mouse 3T3-L1 adipocytes and db/db obesity mice. S. herbacea extract and IR3G inhibited lipase in a concentration-dependent manner. Oil Red O staining disclosed that S. herbacea extract and IR3G significantly suppressed lipid accumulation and adipogenesis and also inhibited the expression of the C/EBPα in the 3T3-L1 adipocytes. In experiments using db/db mice, administering of S. herbacea extract limited body weight gain and significantly reduced feed efficiency and adipose tissue weight. Moreover, analyzing blood triglycerides, total cholesterol, high-density lipoprotein, and low-density lipoprotein, it was confirmed that LDL was significantly decreased and total cholesterol slightly reduced by S. herbacea extract. However, there was no significant change by S. herbacea extract in the changes in blood levels of leptin and adiponectin. Taken together, these results suggest that S. herbacea extract and IR3G inhibit adipogenesis by suppressing the pro-adipogenic transcription factors in 3T3-L1 preadipocytes and prevent obesity by regulating the blood lipid profile as well as the weight of adipose tissue.