bluuebunny/crossref_metadata_2025 huggingface dataset:
Spectris™ treatment preserves corpus callosum structure in Alzheimer's disease
Authors: Da Xiao , Hempel Evan , Brickman Adam M. , Hajós Mihály , Kern Ralph , Cimenser Aylin
Published: October 2024

Objective

To examine the impact of 40Hz gamma stimulation on the preservation of the corpus callosum, a critical structure for interhemispheric connectivity, in people with mild cognitive impairment or Alzheimer's disease.

Methods

OVERTURE (NCT03556280) participants were randomized 2:1 (Active:Sham) to receive daily, 1-h, 40Hz gamma sensory stimulation or sham treatment for 6 months. Structural magnetic resonance imaging data were analyzed to assess changes in corpus callosum area (N = 50; 33 for active, 17 for sham). Bayesian linear mixed-effects modeling was used to assess differences in longitudinal changes of corpus callosum area between the two treatment groups.

Results

All observed differences in corpus callosum area favored the active treatment group. Differences were observed in the total corpus callosum area (2.28 ± 0.87%, p < 0.02) and its subregions, including genu/rostrum (2.36 ± 0.90%, p < 0.02), anterior-body (2.64 ± 1.26%, p < 0.04), mid-body (2.79 ± 1.18%, p < 0.03), posterior-body (2.87 ± 1.41%, p < 0.05), and splenium (1.58 ± 0.73%, p < 0.04). Total corpus callosum area and some of the sub-regional differences, such as genu/rostrum and splenium, were observed as early as 3 months after commencement of treatment.

Interpretation

The structural magnetic resonance imaging results from the OVERTURE Phase 2 study suggest that 6 months of non-invasive 40Hz stimulation reduces the rate of atrophy of the corpus callosum in individuals with Alzheimer's disease. The preservation of structural integrity in the corpus callosum, crucial for interhemispheric communication and cognitive function, may be achievable through this non-invasive approach, potentially providing a promising disease-modifying alternative in Alzheimer's disease management.
Study protocol: Cerebral autoregulation, brain perfusion, and neurocognitive outcomes after traumatic brain injury -CAPCOG-TBI
Authors: Caldas Juliana , Cardim Danilo , Edmundson Philip , Morales Jill , Feng Aaron , Ashley John Devin , Park Caroline , Valadka Alex , Foreman Michael , Cullum Munro , Sharma Kartavya , Liu Yulun , Zhu David , Zhang Rong , Ding Kan
Published: October 2024

Background

Moderate–severe traumatic brain injury (msTBI) stands as a prominent etiology of adult disability, with increased risk for cognitive impairment and dementia. Although some recovery often occurs within the first year post-injury, predicting long-term cognitive outcomes remains challenging, partly due to the significant pathophysiological heterogeneity of TBI, including acute cerebrovascular injury. The primary aim of our recently funded study, cerebral autoregulation, brain perfusion, and neurocognitive outcomes after traumatic brain injury (CAPCOG-TBI), is to determine if acute cerebrovascular dysfunction after msTBI measured using multimodal non-invasive neuromonitoring is associated with cognitive outcome at 1-year post-injury.

Methods

This longitudinal observational study will be conducted at two Level 1 trauma centers in Texas, USA, and will include adult patients with msTBI, and/or mild TBI with neuroimaging abnormalities. Multimodal cerebral vascular assessment using transcranial Doppler and cerebral near-infrared spectroscopy (NIRS) will be conducted within 7-days of onset of TBI. Longitudinal outcomes, including cognitive/functional assessments (Glasgow Outcome Scale and Patient-Reported Outcomes Measurement Information System), cerebral vascular assessment, and imaging will be performed at follow-ups 3-, 6-, and 12-months post-injury. We aim to recruit 100 subjects with msTBI along with 30 orthopedic trauma controls (OTC). This study is funded by National Institute of Neurological Disease and Stroke (NINDS) and is registered on Clinicaltrial.org (NCT06480838).

Expected results

We anticipate that msTBI patients will exhibit impaired cerebrovascular function in the acute phase compared to the OTC group. The severity of cerebrovascular dysfunction during this stage is expected to inversely correlate with cognitive and functional outcomes at 1-year post-injury. Additionally, recovery from cerebrovascular dysfunction is expected to be linked to cognitive recovery.

Conclusion

The results of this study could help to understand the contribution of cerebrovascular dysfunction to cognitive outcomes after TBI and pave the way for innovative vascular-focused interventions aimed at enhancing cognitive recovery and mitigating neurodegeneration following msTB. In addition, its focus toward personalized medicine to aid in the management and prognosis of TBI patients.
Effects of motor imagery training on generalization and retention for different task difficulties
Authors: Sato Yoichiro
Published: October 2024

Although previous studies have suggested that motor adaptation through motor imagery training of similar tasks can improve retention and generalization of motor learning, the benefits of mental and physical training remain unclear for different task difficulties. Two experiments were conducted in this study. The first experiment aimed to determine whether there were differences in movement time (MT) when drawing circles based on three conditions in accordance with Fitts’ law. The results showed significant differences in MT among the three conditions (p < 0.001), with MT becoming long as the width of the circle line (which indicated different difficulty level) narrowed. The second experiment aimed to determine whether the task difficulty influenced immediate generalization and retention at 24 h after mental vs. physical training. Participants in both training groups practiced the task with the medium-sized circle, which indicated medium difficulty. The posttest results revealed that mental training leads to considerable performance improvement than physical training, as demonstrated by a shorter MT regardless of the task difficulty level. Meanwhile, the retention test results showed no difference in generalization between mental and physical training. However, generalization of an easier task was more effectively retained than more difficult tasks. These results suggest that mental training can improve performance during the adaptation phase and that difficulty level can influence the degree of retention.
The multifaceted role of quaking protein in neuropsychiatric disorders and tumor progression
Authors: Guo Zeshang , Liu Bo , Wei Ying , Wang HeFei , Zhang Qingquan , Hong Xinyu
Published: October 2024

The Quaking protein (QKI) belongs to the STAR protein family and plays a significant role in the development of the nervous system. It serves as a crucial regulator in the processes of tumor progression and cardiovascular system development. Within the central nervous system, QKI has been associated with the onset and progression of numerous neuropsychiatric disorders, including schizophrenia, depression, ataxia, and Alzheimer’s disease. In malignant tumors, the methylation of the QKI promoter inhibits its expression. QKI primarily involves in the generation, stability, and selective splicing of non-coding RNA, as well as in mRNA translation. The role of QKI in the tumor microenvironment should not be overlooked. Especially in Glioblastoma Multiforme (GBM), although QKI is not the primary mutation, it still plays a vital role in maintaining the stemness of GBM. However, the mechanisms and further studies on this topic demand extensive basic and clinical trials.
Contribution of direct-drinking water to calcium and magnesium and the influence on the height in school-age children
Authors: Gu Hongru , Gong Yijing , Li Zhao , Zhang Yanqiu , Wu Jin , Wang Yi , Ni Min , Zhang Jun , Jiang Hai
Published: October 2024

Objective

To estimate the contribution of direct-drinking water provided in school to dietary intake (DI) and recommended nutrient intake (RNI) of calcium and magnesium, and to explore its influence on the height in school-age children.

Methods

Tap water and direct-drinking water samples were collected from schools in Taicang City to test the contents of calcium and magnesium, and compared by one-way ANOVA and post-hoc test. Contribution rates of direct-drinking water to DI and RNI were calculated by using the contents of calcium and magnesium and data from the Nutrition and Health Status Survey 2021. A retrospective cohort was conducted among 4,850 first-grade children consuming direct-drinking water in Taicang City from 24 primary schools in 2019. Group 1 (1,070 boys and 946 girls) consumed UF-process water with normal calcium and magnesium contents and Group 2 (1,548 boys and 1,286 girls) consumed NF/RO-process water with very low calcium and magnesium contents. During 2019–2023, the height and height growth were analyzed with the Student’s t-test.

Results

The highest calcium content was examined in tap water samples, followed by direct-drinking water samples supplied through a UF, NF, and RO system (F = 1,227.725, p < 0.001). The highest magnesium content was examined in water supplied through a UF system, followed by that through a tap, NF and RO system (F = 146.504, p < 0.001). Calcium and magnesium contents in direct-drinking water supplied through a UF system changed little compared with those in tap water, which were significantly reduced in direct-drinking water supplied through a NF and RO system. The contribution rates of direct-drinking water to DI of calcium and magnesium were 8.95 and 2.78%, respectively, and those to RNI of calcium and magnesium were 2.63 and 1.96%, respectively. There were no significant differences in the height and height growth of first-grade children drinking water supplied through the UF system vs. NF/RO system (p > 0.05).

Conclusion

Direct-drinking water processed through a NF or RO system should be cautiously adopted in primary and secondary schools. A UF system is preferred in schools where no health concerns are associated with water quality.
Low dietary magnesium and fiber intakes among women with metabolic syndrome in Kuwait
Authors: Alkazemi Dalal U. Z. , Zafar Tasleem A. , Alsouri Nourah Y. , Aljahdali Abeer A. , Kubow Stan
Published: October 2024

Introduction

Metabolic syndrome (MetS) is a growing health concern among Kuwaiti women. Dietary magnesium and fiber have been implicated in reducing the risk of MetS; however, their specific effects on this population remain underexplored. This study aimed to investigate the association of dietary magnesium and fiber intake with the prevalence of MetS and its components among women in Kuwait.

Methods

This study included 170 women aged 18–65 (years) recruited from AL-Adan Hospital, Mubarak Hospital, and Riqqa Polyclinic. Data were collected using a modified Semi-Quantitative Food Frequency Questionnaire (SFFQ) to assess dietary intake, and biochemical measurements were performed to evaluate serum magnesium and other metabolic markers. MetS was diagnosed according to International Diabetes Federation (IDF) and Adult Treatment Panel III (ATP III) criteria. Statistical analyses included Mann–Whitney U-tests, chi-square tests, Spearman correlations, logistic and linear regression models, and Cohen’s kappa statistics.

Results

The prevalence of MetS was 24 and 18% based on the IDF and ATP III criteria, respectively. Women with MetS had significantly lower dietary magnesium and fiber intakes than those in women without MetS (p < 0.001). A strong positive correlation was found among dietary magnesium intake, fiber intake, and serum magnesium levels (r = 0.957, p < 0.001 for magnesium; r = 0.917, p < 0.001 for fiber). Increased dietary magnesium and fiber intakes were linked to reduced odds of developing MetS and its components, except for blood pressure measurements. Cohen’s kappa demonstrated a strong agreement (K = 0.70, p < 0.001) between dietary and serum magnesium inadequacy.

Conclusion

Increased dietary intakes of magnesium and fiber are associated with reduced odds of developing MetS among Kuwaiti women. These findings support the promotion of magnesium- and fiber-rich diets as preventive strategies against MetS.
Food addiction in patients on weight loss treatment
Authors: Palacio Ana Cristina , Cuello Catalina , Díaz-Torrente Ximena
Published: October 2024

Background

Food addiction (FA) is defined as hedonic eating behavior involving the consumption of highly palatable foods (i.e., ultra processed foods in quantities beyond homeostatic energy requirements). FA is present in a subset of patients with overweight or obesity and could contribute to the response to weight loss treatments.

Aim

Determine FA in individuals that fulfil the criteria of FA as measured by the YFAS 2.0, and its association with anthropometric and body composition variables in a clinical sample of patients undergoing weight loss treatment. Also, to determine the most prevalent FA criteria.

Methods

An observational, analytical, and cross-sectional study was conducted. Sampling was non-probabilistic, convenience based. A total of 158 participants were recruited from two clinical centers (private and public) focused on managing non-communicable chronic diseases. The Chilean version of YFAS 2.0 was administrated through the online REDCap platform. Anthropometric measurements were measured, and body mass index (BMI) was calculated.

Results

The mean age was 47.8 (SD 14.9) and BMI 28.7 (SD 5.3) kg/m2. There were 12.7% patients who screened positive on the YFAS 2.0 Chilean version (3.2% for mild, 2.5% for moderate and 7.0% for severe), the mean symptom count was 2.2 (SD 2.6), with withdrawal being the most prevalent criterion (33.5%). FA patients had significantly higher body weight compared to non-FA subjects (p = 0.045). We observed a slight and significant correlation between FA symptom count and body weight (p = 0.020), waist circumference (p = 0.005), and BMI (p = 0.023).

Conclusion

This is the first study in Chile that showed that FA was present in patients undergoing weight loss treatment and was associated with anthropometric measurements. In addition, it showed that withdrawal was the most prevalent criterion. Future studies should investigate whether the presence of FA and the withdrawal criterion could contribute to suboptimal clinical response to weight loss treatment.
Association between composite dietary antioxidant index and fatty liver index among US adults
Authors: Zheng Meng , Li Chaochen , Fu Jia , Bai Long , Dong Jinghui
Published: October 2024

Background

The potential beneficial health effects of dietary antioxidants have been reported. However, the association of a composite dietary antioxidant index (CDAI) with fatty liver index (FLI) remains unclear. This study aims to assess whether CDAI (including its components) is associated with FLI among US adults.

Methods

This population-based cross-sectional study used data on US adults from the National Health and Nutrition Examination Survey (NHANES) 2007–2018 cycles. Weighted generalized linear regression models were used to analyze the association between CDAI (including vitamin A, C, E, zinc, selenium, and carotenoids) and FLI, which was calculated by using body mass index (BMI), waist circumference and levels of γ-glutamyl transferase and triglycerides.

Results

Weighted generalized linear regression models showed an inverse association between CDAI and FLI in the total population (β, −0.40; 95% CI, −0.59, −0.21), in women (β, −0.56; 95% CI, −0.94, −0.18), and in men (β, −0.32; 95% CI, −0.54, −0.10) after adjusting for various confounders. The restricted cubic splines showed the negative linear dose–response associations between CDAI and FLI (all P non_linear >0.05). The dietary selenium intake in women has an inverse U-shaped relationship with FLI, with an inflection point value of 110 μg. In model 3, intake of dietary antioxidants Vitamins A, C, E, and carotenoids were significantly negatively associated with FLI in female but only were vitamins A and E negatively associated with FLI in male. In subgroup analysis, CDAI showed a significantly negative relation to FLI among those aged 60 years or older (β, −0.57; 95% CI, −0.81, −0.33), among those who engaged in active physical activity (β, −0.46; 95% CI, −0.63, −0.29), among those without metabolic syndrome (β, −0.43; 95% CI, −0.62, −0.24), and those without hyperuricemia (β, −0.43; 95% CI, −0.60, −0.26). Additionally, CDAI was significantly negatively associated with male FLI, regardless of whether they had diabetes or not.

Conclusion

In conclusion, our results indicate that higher CDAI may be associated with a lower FLI.
A low-carbohydrate, high-fat diet leads to unfavorable changes in blood lipid profiles compared to carbohydrate-rich diets with different glycemic indices in recreationally active men
Authors: Kripp Anna Maria , Feichter Andreas , König Daniel
Published: October 2024

Objective

In addition to recent discussions of low-carbohydrate, high-fat diets (LCHF) from a performance perspective, there is a paucity of knowledge regarding influence of the combined effect of an exercise and nutritional intervention, which varies in carbohydrate (CHO) intake and glycemic indices, on blood lipid levels in recreationally active men.

Methods

A total of 65 male runners (VO2 peak = 55 ± 8 mL·min−1·kg−1) completed a 10-week ad libitum nutritional regimen (LOW-GI: ≥ 65% low GI CHO per day, n = 24; HIGH-GI: ≥ 65% high GI CHO per day, n = 20; LCHF: ≤ 50 g CHO daily, n = 21) with a concurrent prescribed endurance training intervention. Fasting total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were determined before and after the intervention. Additionally, 24-h dietary recalls were completed twice weekly.

Results

Following the intervention, TC was significantly higher in LCHF (196 ± 37 mg·dL−1) compared to both LOW-GI (171 ± 41 mg·dL−1) and HIGH-GI (152 ± 28 mg·dL−1, p < 0.001). Additionally, LDL-C levels increased in LCHF (+17 ± 21 mg·dL−1, p = 0.001), while they decreased in both CHO groups (p < 0.05, respectively). Only the HIGH-GI group demonstrated a significant reduction in HDL-C (−3 ± 9 mg·dL−1, p = 0.006), while a decrease in TG was only significant in LOW-GI (−18 ± 36 mg·dL−1, p = 0.008).

Conclusion

Although mean blood lipid levels remained within the normal range, the data indicate that a low-carbohydrate, high-fat (LCHF) diet leads to unfavorable changes in individual blood lipid profiles compared to carbohydrate-rich diets. Therefore, it is recommended that the impact of a low-carbohydrate diet on blood lipids be considered when counseling active and healthy individuals.
From trait affect to situated performance of change-oriented organizational citizenship behavior: the pathway through situational construal and emotion utilization
Authors: Zampetakis Leonidas A. , Arvanitis Alexios
Published: October 2024

Prior studies indicate that fostering change-oriented organizational citizenship behavior (OCB-CH) among employees, wherein they actively contribute to the collective welfare of modern organizations, correlates positively with their positive affect. However, the precise mechanisms linking trait positive affect with OCB-CH remain unclear. This study used aggregated 5-day daily diary data from a sample of 236 full-time employees in Greece to examine positive affectivity differences in the characteristics of situational experiences and how these differences relate to employee OCB-CH via the use of emotions to facilitate performance. Employing multilevel structural equation modeling, the findings revealed a positive association between trait positive affectivity and the situation characteristics of duty, intellect, and positivity, from the DIAMONDS taxonomy. Furthermore, it was determined that perceptions of situational characteristics and emotional utilization sequentially mediated the relationship between positive affectivity and OCB-CH. This study enhances our understanding of how employee trait positive affectivity influences the manifestation of positive change in the form of OCB-CH within organizations.
Rescue analgesia with a transversus abdominis plane block alleviates moderate-to-severe pain and improves oxygenation after abdominal surgery: a randomized controlled trial
Authors: He Jingxian , Qin Shuai , Wang Yuwen , Ye Qiuping , Wang Penglei , Zhang Ye , Wu Yun
Published: October 2024

Background

Abdominal surgery is a common surgical procedure that is frequently associated with substantial postoperative pain. However, rescue analgesia using opioids is associated with several adverse effects. The transversus abdominis plane block (TAPB) has been demonstrated to be effective as part of multimodal analgesia. This study aims to evaluate the effects of rescue analgesia using the TAPB following abdominal surgery.

Methods

Ninety patients undergoing abdominal surgery and reporting a numeric rating scale (NRS) score of cough pain ≥4 on the first postoperative day were randomized to receive either sufentanil or TAPB for rescue analgesia. Pain scores and arterial oxygen pressure (PaO2) were evaluated before and after the administration of rescue analgesia. Sleep quality and gastrointestinal function were assessed postoperatively. The primary outcome was the degree of pain relief on coughing 30 min after the administration of rescue analgesia.

Results

Patients of both groups reported a significantly reduced NRS score on coughing 30 min after receiving rescue analgesia (Ppaired < 0.001 for both groups). Notably, the degree of pain relief was significantly higher in the TAPB group than in the sufentanil group [median (interquartile range), −3 (−4 to −2) vs. −2 (−2 to −1), median difference = −1; 95% confidence interval, −2 to −1; P < 0.001]. Moreover, patients in the TAPB group experienced less pain than those in the sufentanil group during the following 24 h. When evaluated, PaO2 increased significantly after rescue analgesia was administered in the TAPB group (Ppaired < 0.001); however, there were no significant intragroup differences in the sufentanil group (Ppaired = 0.129). Patients receiving the TAPB experienced better quality of sleep than those receiving sufentanil (P = 0.008), while no statistical differences in gastrointestinal function were observed between the two groups.

Conclusion

Rescue analgesia with the TAPB on the first postoperative day alleviated pain, enhanced oxygenation, and improved sleep quality in patients undergoing abdominal surgery; however, its effect on gastrointestinal function requires further research.

Clinical Trial Registration

This study was registered in the Chinese Clinical Trial Registry (https://www.chictr.org.cn/showproj.html?proj=170983, ChiCTR2200060285) on 26 May 2022: Patients were recruited during the period between 30 May 2022 and 14 February 2023, and a follow-up of the last enrolled patient was completed on 16 March 2023.
45,X[2]/46,X,der(Y).ish Psu idic(Y)(q11.2)[38] mosaic karyotype in mixed gonadal dysgenesis: a case report and literature review
Authors: Zhang Qiang , Chen Xiaoxiao , Cao Yanyan , Zhou Yun , Liu Yingye , Liu Lijun , Liu Lei , Cui Xiaowei
Published: October 2024

Mixed gonadal dysgenesis is caused by a variety of chromosome abnormalities, most commonly Y chromosome mosaicism. An 8-year-old boy presented with short stature for possible treatment with recombinant growth hormone. He had a history of mixed gonadal dysgenesis (hypospadias, bilateral cryptorchidism, processus vaginalis, and dysplastic immature uterus) and a series of corrective surgeries. At 14 months of age, chromosomal karyotyping revealed 46,X,+mar. Upon presentation, lab testing was consistent with the male phenotype at prepuberty. Fluorescence in situ hybridization revealed 45,X[2]/46,X,der(Y).ish psu idic(Y)(q11.2)(SRY++,DYZ3++)[38] karyotype. A literature review identified eight case reports of mixed gonadal dysgenesis associated with 45,X/46,X,idic(Y)(q11.2). Neither sex phenotype nor short stature correlated with the 46,X,idic(Y)(q11.2) mosaic ratio.
Effectiveness of biologic therapies in achieving treatment targets in inflammatory bowel disease; real-world data from the Middle East (ENROLL study)
Authors: Shehab Mohammad , Alfadhli Ahmad , Abdullah Israa , Alostad Wrood , Marei Alaa , Alrashed Fatema
Published: October 2024

Background

Real-world data assessing the effectiveness of biologics in patients with inflammatory bowel disease (IBD) in the Middle East are not well-established. In our study, we evaluated the effectiveness of biologic therapies in achieving clinical and endoscopic outcomes in biologic-naïve patients with IBD.

Design

A retrospective chart review was conducted at two tertiary care gastroenterology centers using electronic medical records of patients with moderate-to-severe IBD. The study period was from October 2017 to October 2023. Patients who were on infliximab, adalimumab, ustekinumab, or vedolizumab for 12 months were included in the analysis. The primary outcomes were the percentage of IBD-related hospitalizations or surgeries, achieving steroid-free remission, and endoscopic remission.

Results

A total of 422 patients were included in the study, of whom 264 (62.5%) patients had Crohn’s disease (CD) and 158 (39%) had ulcerative colitis (UC). In patients with CD, endoscopic remission was attained in 51 (52%) of the patients on adalimumab, 38 (53%) of the patients on infliximab, 34 (56%) of the patients on ustekinumab, and 16 (51%) of the patients on vedolizumab. In patients with UC, endoscopic remission was attained in 40 (56%) of the patients on infliximab, 26 (61%) of the patients on adalimumab, 8 (55%) of the patients on ustekinumab, and 11 (53%) of the patients on vedolizumab.

Conclusion

adalimumab, infliximab, ustekinumab, and vedolizumab were all effective in achieving clinical and endoscopic clinical outcomes in IBD in both UC and CD. The findings of this study suggest that the efficacy of biologics in a Middle Eastern population is similar to that in a Western population.
Flavonoids against depression: a comprehensive review of literature
Authors: Alizadeh Alaleh , Pourfallah-Taft Yeganeh , Khoshnazar Maryam , Safdari Aysan , Komari Saba Vafadar , Zanganeh Mehrnaz , Sami Nafiseh , Valizadeh Maryam , Faridzadeh Arezoo , Alijanzadeh Dorsa , Mazhari Seyed Amirhossein , Khademi Reza , Kheirandish Ali , Naziri Mahdyieh
Published: October 2024

Background

Depression is a state of low mood and aversion to activity, which affects a person’s thoughts, behavior, motivation, feelings, and sense of wellbeing. Pharmacologic therapies are still the best effective treatment of depression. Still, most antidepressant drugs have low efficacy and delayed onset of therapeutic action, have different side effects, and even exacerbate depression. Such conditions make it possible to look for alternatives. Consequently, we decided to summarize the impact of flavonoids on depression in this review.

Methods

We searched scientific databases such as SCOPUS, PubMed, and Google Scholar to find relevant studies until July 2022.

Results

A wide variety of natural components have been shown to alleviate depression, one of which is flavonoids. Due to the growing tendency to use natural antidepressant drugs, scientific studies are increasingly being conducted on flavonoids. This study aims to review the latest scientific researches that indicate the antidepressant potential of flavonoids. Various mechanisms include neurotransmitter system modulation and dopaminergic, noradrenergic, and serotonergic pathways regulation in the central nervous system. Different compounds of flavonoids have antidepressant properties in vivo or in vitro experiments or clinical trials and can be used as alternative and complementary treatments for depression. In general, it was observed that there were no severe side effects.

Conclusion

Our study proves the antidepressant potential of flavonoids, and considering the limited side effects, they can be used as complementary medicine for depressed patients.
Proposed mechanisms of action participating in the hypoglycemic effect of the traditionally used Croton guatemalensis Lotsy and junceic acid, its main compound
Authors: Moreno-Vargas Angelina Daniela , Andrade-Cetto Adolfo , Espinoza-Hernández Fernanda Artemisa , Mata-Torres Gerardo
Published: October 2024

Croton guatemalensis Lotsy (Euphorbiaceae) is an important traditional medicine that is used by the Cakchiquels of Guatemala to control hyperglycemia in patients with type 2 diabetes. Previous studies have shown that administration of this plant induces an acute hypoglycemic effect during fasting and that the main compound is junceic acid, a diterpenoid with a clerodane skeleton; however, junceic acid has not been reported to have hypoglycemic activity in the literature. As the mechanisms involved in the hypoglycemic effect of C. guatemalensis remain unknown, the objective of the present investigation was to elucidate the hypoglycemic mechanisms of this species, as well as its major compound, junceic acid. The results indicated that, similar to complete extract, junceic acid exhibited a hypoglycemic effect in hyperglycemic rats. Both C. guatemalensis extract and junceic acid inhibited the activity of two rate-limiting enzymes involved in hepatic glucose production; however, compared with chlorogenic acid, junceic acid had a more potent effect on glucose-6-phosphatase levels than chlorogenic acid, which was used as a positive control. Furthermore, both fasting and postprandial insulin levels decreased in healthy and hyperglycemic rats despite reduced blood glucose levels in both metabolic states, suggesting a potential insulin-sensitizing effect. However, neither of these compounds potentiated the effect of insulin in insulin tolerance tests nor inhibited the enzyme activity of protein tyrosine phosphatase 1B, a negative regulator of the insulin pathway. Therefore, the insulin-sensitizing effect is thought to be independent of insulin and mediated by potential activation of the AMP-activated protein kinase pathway. The specific activation of this master regulator in β-cells results in the inhibition of insulin secretion in a healthy state and the restoration of the insulin response under conditions of glucotoxicity; these effects were observed after the administration of the extract and junceic acid in healthy and hyperglycemic rats. Overall, the main findings of this study establish a basis of the mechanisms of action of C. guatemalensis and its main compound, junceic acid, in terms of their hypoglycemic effect.
Retinoid X receptor heterodimers in hepatic function: structural insights and therapeutic potential
Authors: Xu Renjie , Zhang Linyue , Pan Hao , Zhang Yong
Published: October 2024

Nuclear receptors (NRs) are key regulators of multiple physiological functions and pathological changes in the liver in response to a variety of extracellular signaling changes. Retinoid X receptor (RXR) is a special member of the NRs, which not only responds to cellular signaling independently, but also regulates multiple signaling pathways by forming heterodimers with various other NR. Therefore, RXR is widely involved in hepatic glucose metabolism, lipid metabolism, cholesterol metabolism and bile acid homeostasis as well as hepatic fibrosis. Specific activation of particular dimers regulating physiological and pathological processes may serve as important pharmacological targets. So here we describe the basic information and structural features of the RXR protein and its heterodimers, focusing on the role of RXR heterodimers in a number of physiological processes and pathological imbalances in the liver, to provide a theoretical basis for RXR as a promising drug target.
Pharmacokinetics and bioequivalence of two formulations of mifepristone tablets in healthy Chinese subjects under fasting conditions: a single-center, open, randomized, single-dose, double-period, two-sequence, crossover trial
Authors: Yan Yufeng , Zhu Xiaoshan , Dong Ping , Liu Cheng , Lu Lingqing , Zeng Liyan , Chen Guiying , Meng Xianmin , Liu Min
Published: October 2024

Objective

A bioequivalence (BE) study was performed to evaluate the pharmacokinetics, safety, and bioequivalence of two formulations of mifepristone tablets in healthy Chinese volunteers under fasting conditions.

Methods

A single-center, open, randomized, single-dose, double-period, two-sequence, crossover study in healthy subjects under fasting conditions was performed. The subjects received a single fasting dose of mifepristone (10 mg/tablet) during the first and second periods, followed by a 14-day washout period, during which frequent pharmacokinetic (PK) sampling occurred up to 120 h. The pharmacokinetic parameters of mifepristone were calculated based on the plasma drug concentration–time profile. Primary endpoints were the BE of major pharmacokinetic parameters (AUC0-t and AUC0-∞) and the maximum observed serum concentration (Cmax). Secondary endpoints were safety parameters.

Results

Forty subjects (34 male and 6 female subjects) were randomly assigned to treatment, with 39 completing the two-period study. After the single administration of mifepristone tablets (test preparation vs. reference preparation) under fasting conditions, the geometric mean ratios (GMRs) of Cmax, AUC0-t, and AUC0-∞ were 98.76%, 104.28%, and 104.83%, respectively. The primary metabolites of mifepristone (RU42633 and RU42698),the GMRs of Cmax, AUC0–t, AUC0–∞ were 102.33% and 100.97%, 103.17% and 103.71%, 104.02% and 103.84%, respectively. Similarly, for another metabolite of mifepristone (RU42698), the GMRs of Cmax, AUC0-t, and AUC0-∞ were 100.97%, 103.71%, and 103.84%, respectively. All 90% confidence intervals (CIs) for the test/reference AUC ratio and Cmax ratio were within the acceptable range (80%–125%) for BE, which met the requirements of bioequivalence. No serious adverse events (AEs) occurred, and all AEs were classified as level 1 or 2.

Conclusion

The PK parameters of mifepristone and its metabolites (RU42633 and RU42698) were measured using the (GMRs) of AUC0-t, AUC0-∞, and Cmax and were similar between the test and reference drug. The two formulations of mifepristone showed good tolerability and a similar safety profile.

Clinical Trial Registration

chinadrugtrials.org.cn, identifier CTR20182413.
Modeling and characterization of pure and odorant mixture processing in the Drosophila mushroom body calyx
Authors: Lazar Aurel A. , Liu Tingkai , Yeh Chung-Heng , Zhou Yiyin
Published: October 2024

Associative memory in the Mushroom Body of the fruit fly brain depends on the encoding and processing of odorants in the first three stages of the Early Olfactory System: the Antenna, the Antennal Lobe and the Mushroom Body Calyx. The Kenyon Cells (KCs) of the Calyx provide the Mushroom Body compartments the identity of pure and odorant mixtures encoded as a train of spikes. Characterizing the code underlying the KC spike trains is a major challenge in neuroscience. To address this challenge we start by explicitly modeling the space of odorants using constructs of both semantic and syntactic information. Odorant semantics concerns the identity of odorants while odorant syntactics pertains to their concentration amplitude. These odorant attributes are multiplicatively coupled in the process of olfactory transduction. A key question that early olfactory systems must address is how to disentangle the odorant semantic information from the odorant syntactic information. To address the untanglement we devised an Odorant Encoding Machine (OEM) modeling the first three stages of early olfactory processing in the fruit fly brain. Each processing stage is modeled by Divisive Normalization Processors (DNPs). DNPs are spatio-temporal models of canonical computation of brain circuits. The end-to-end OEM is constructed as cascaded DNPs. By extensively modeling and characterizing the processing of pure and odorant mixtures in the Calyx, we seek to answer the question of its functional significance. We demonstrate that the DNP circuits in the OEM combinedly reduce the variability of the Calyx response to odorant concentration, thereby separating odorant semantic information from syntactic information. We then advance a code, called first spike sequence code, that the KCs make available at the output of the Calyx. We show that the semantics of odorants can be represented by this code in the spike domain and is ready for easy memory access in the Mushroom Body compartments.
Hydroponic lettuce in-situ water circulation evaluation via nondestructive mass measurement in controlled environment
Authors: Huang Yanhua , Ni Zheng , Chang Yanbin , Wang Lizhi
Published: October 2024

This study proposed a hydroponic system with the capacity to acquire high-resolution in situ mass data for non-destructive evaluation of water circulation in lettuce. The system customizes the watering profile, enables high-frequency in situ weight measurement, and monitors multidimensional environment changes. Key air, water, and light parameters were collected to evaluate the plant response, susceptibility, and adaptability to environmental conditions. Multiple physiological indices were defined to characterize the properties of two lettuce varieties in response to different environmental factors.
A multi-omics insight on the interplay between iron deficiency and N forms in tomato
Authors: Lodovici Arianna , Buoso Sara , Miras-Moreno Begoña , Lucini Luigi , Tomasi Nicola , García-Pérez Pascual , Pinton Roberto , Zanin Laura
Published: October 2024

Introduction

Nitrogen (N) and iron (Fe) are involved in several biochemical processes in living organisms, and their limited bioavailability is a strong constraint for plant growth and yield. This work investigated the interplay between Fe and N nutritional pathways in tomato plants kept under N and Fe deficiency and then resupplied with Fe and N (as nitrate, ammonium, or urea) through a physiological, metabolomics and gene expression study.

Results

After 24 hours of Fe resupply, the Fe concentration in Fe-deficient roots was dependent on the applied N form (following the pattern: nitrate > urea > ammonium > Fe-deficient control), and whereas in leaves of urea treated plants the Fe concentration was lower in comparison to the other N forms. Untargeted metabolomics pointed out distinctive modulations of plant metabolism in a treatment-dependent manner. Overall, N-containing metabolites were affected by the treatments in both leaves and roots, while N form significantly shaped the phytohormone profile. Moreover, the simultaneous application of Fe with N to Fe-deficient plants elicited secondary metabolites’ accumulation, such as phenylpropanoids, depending on the applied N form (mainly by urea, followed by nitrate and ammonium). After 4 hours of treatment, ammonium- and urea-treated roots showed a reduction of enzymatic activity of Fe(III)-chelate reductase (FCR), compared to nitrate or N-depleted plants (maintained in Fe deficiency, where FCR was maintained at high levels). The response of nitrate-treated plants leads to the improvement of Fe concentration in tomato roots and the increase of Fe(II) transporter (IRT1) gene expression in tomato roots.

Conclusions

Our results strengthen and improve the understanding about the interaction between N and Fe nutritional pathways, thinning the current knowledge gap.
Hyperspectral indices data fusion-based machine learning enhanced by MRMR algorithm for estimating maize chlorophyll content
Authors: Nagy Attila , Szabó Andrea , Elbeltagi Ahmed , Nxumalo Gift Siphiwe , Bódi Erika Budayné , Tamás János
Published: October 2024

Accurate estimation of chlorophyll is essential for monitoring maize health and growth, for which hyperspectral imaging provides rich data. In this context, this paper presents an innovative method to estimate maize chlorophyll by combining hyperspectral indices and advanced machine learning models. The methodology of this study focuses on the development of machine learning models using proprietary hyperspectral indices to estimate corn chlorophyll content. Six advanced machine learning models were used, including robust linear stepwise regression, support vector machines (SVM), fine Gaussian SVM, Matern 5/2 Gaussian stepwise regression, and three-layer neural network. The MRMR algorithm was integrated into the process to improve feature selection by identifying the most informative spectral bands, thereby reducing data redundancy and improving model performance. The results showed significant differences in the performance of the six machine learning models applied to chlorophyll estimation. Among the models, the Matern 5/2 Gaussian process regression model showed the highest prediction accuracy. The model achieved R2 = 0.71 for the training set, RMSE = 338.46 µg/g and MAE = 264.30 µg/g. In the case of the validation set, the Matern 5/2 Gaussian process regression model further improved its performance, reaching R2 =0.79, RMSE=296.37 µg/g, MAE=237.12 µg/g. These metrics show that Matern’s 5/2 Gaussian process regression model combined with the MRMR algorithm to select optimal traits is highly effective in predicting corn chlorophyll content. This research has important implications for precision agriculture, particularly for real-time monitoring and management of crop health. Accurate estimation of chlorophyll allows farmers to take timely and targeted action.
Relationship between root system-soil C:N:P and soil microbial diversity at different evolutionary stages of Caragana tibetica scrub in arid desert grassland, Northern China
Authors: Han Min , Liang Yumei , Gao Yong , Yang Wenyuan , Guo Yuefeng
Published: October 2024

Scrub root systems play a crucial role in preventing soil erosion and nutrient loss. However, the effects of the root system configuration on soil nutrient dynamics and microbial changes at various evolutionary stages remain poorly understood. In this study, we investigated the relationship between soil physical and chemical properties and the diversity of bacteria and fungi throughout the evolutionary stages of the scrub root systems. This was achieved through a combination of whole-root excavation and root tracing techniques. The results indicated that root diameter was the main factor contributing to the continuous increase in carbon (C): phosphorus (P) and nitrogen (N): P ratios as the scrub sand pile developed. The soil organic carbon (SOC), total soil nitrogen (TN), and total soil phosphorus (TP) contents in the soil in the four evolutionary stages were the highest during the developmental stage, but the change in TP content was not statistically significant (P > 0.05). Partial least squares path modeling and redundancy analysis (RDA) indicated that root system stoichiometric C and N contents were positively correlated with microbial diversity (R2 = 0.85). There was no correlation between the evolutionary stage and soil nutrient TN, whereas soil nutrient TN was negatively correlated with microbial diversity (R2 = −0.92). These findings elucidate the relationship between the evolutionary stage of root chemical measurement characteristics, soil elements and microorganisms, and their subsequent effects on root elemental composition and microbial diversity. This study enhances the current understanding of plant–soil interactions in desert steppe ecosystems.
Potato growth, nitrogen balance, quality, and productivity response to water-nitrogen regulation in a cold and arid environment
Authors: Su Dandan , Zhang Hengjia , Teng Anguo , Zhang Changlong , Lei Lian , Ba Yuchun , Zhou Chenli , Li Fuqiang , Chen Xietian , Wang Zeyi
Published: October 2024

Background

The pervasively imprudent practices of irrigation and nitrogen (N) application within Oasis Cool Irrigation zones have led to significant soil nitrogen loss and a marked decrease in water and nitrogen use efficiency.

Methods

To address this concern, a comprehensive field experiment was conducted from April to September in 2023 to investigate the impact of varying degrees of water and fertilization regulation strategies on pivotal parameters including potato yield, quality, nitrogen balance, and water-nitrogen use efficiency. The experimental design incorporated two water deficit degrees at potato seedling (W1, 55%-65% of Field Capacity (FC); W2, 45%-55% of FC), and four distinct nitrogen application gradients (N0, 0 kg ha-1 of N; N1, 130 kg ha-1 of N; N2, 185 kg ha-1 of N; N3, 240 kg ha-1 of N). A control was also included, comprising N0 nitrogen application and full irrigation (W0, 65%-75% of FC), totally eight treatments and one check.

Results

The results indicated that the tuber yield, plant dry matter accumulation, plant height, plant stem, and leaf area index increased with higher nitrogen fertilizer application and irrigation volume. However, tuber starch content, vitamin C, and protein content initially increased and then decreased, while reducing sugar content consistently decreased. Except for W1N2 treatment, the irrigation water use efficiency increased as the N application rate rose, while the nitrogen partial factor productivity, crop nitrogen use efficiency and soil nitrogen use efficiency decreased with an increase in N fertilizer application. The W1N2 treatment resulted in a higher yield (43.16 t ha-1), highest crop nitrogen use efficiency (0.95) and systematic nitrogen use efficiency (0.72),while maintaining moderate levels of soil nitrate and ammonium nitrogen.

Conclusion

Therefore, through the construction of an integrated evaluation index (IEI), the W1N2 treatment of mild water deficit (55%-65% of FC) at potato seedling combined with the medium nitrogen application (185 kg ha-1 of N) has the highest IEI (0.978), it was recommended as the optimal water-nitrogen regulation and management strategies to facilitate high-yield, high-efficiency, and environmentally sustainable potato production in the cold and arid oasis areas of northwest China.
Geographical variation and genetic diversity of Parashorea chinensis germplasm resources
Authors: Xu Yuanyuan , Liu Shinan , Finnegan Patrick M. , Liu Fang , Ali Izhar , Zhang Haidong , Yang Mei
Published: October 2024

Introduction

Parashorea chinensis is a rare monodominant species in southwest China known for its production of high-quality timber, is facing decline due to its narrow distribution, human interference and habitat destruction. However, there are no reports on genetic diversity and geographical variation of phenotypic traits of P. chinensis.

Methods

In this study, phenotypic characters and genetic diversity of 15 germplasms resources from five provenances in southwest China were investigated, and their relationships with geographical and environmental factors was discussed.

Results

Our results revealed a rich phenotypic diversity among the germplasms, with variation coefficients ranging from 3.63% to 45.49%. Among the studied germplasms, NP03 from Napo and ML02 from Mengla region exhibited superior phenotypic traits. Notably, NP03 also demonstrated the highest genetic diversity. Genetic differentiation analyses including genetic differentiation coefficient (0.6264) and gene flow (0.3736) illustrated that genetic variation was most prevalent among populations. Furthermore, redundancy analysis showed that temperature related factors (maximum air temperature, annual mean temperature and minimum air temperature) significantly affected phenotypic variation. Similarly, altitude, longitude, latitude, annual mean precipitation and the minimum air temperature significantly impacted the level of genetic diversity. The molecular variation of the natural population of P. chinensis followed a certain geographical pattern.

Discussion

Our finding indicated abundant phenotypic variation among P. chinensis germplasms. However, populations exhibited low levels of genetic diversity alongside high genetic differentiation, potentially contributing to the species' rarity. Based on our results, NP03 and ML02 germplasm could be used as the parents for breeding superior germplasm of P. chinensis. Overall, this study provides valuable insights into germplasm diversity and conservation, genetic improvement, and utilization of P. chinensis.