bluuebunny/crossref_metadata_2025 huggingface dataset:
Aspirations and Worries: The Role of Parental Intrinsic Motivation in Establishing Oral Health Practices for Indigenous Children
Authors: Poirier Brianna F. , Hedges Joanne , Smithers Lisa G. , Moskos Megan , Jamieson Lisa M.
Published: November 2021

Aboriginal and Torres Strait Islander (respectfully, subsequently referred to as Indigenous) children in Australia experience oral disease at a higher rate than non-Indigenous children. A history of colonisation, government-enforced assimilation, racism, and cultural annihilation has had profound impacts on Indigenous health, reflected in oral health inequities sustained by Indigenous communities. Motivational interviewing was one of four components utilised in this project, which aimed to identify factors related to the increased occurrence of early childhood caries in Indigenous children. This qualitative analysis represents motivational interviews with 226 participants and explores parents’ motivations for establishing oral health and nutrition practices for their children. Findings suggest that parental aspirations and worries underscored motivations to establish oral health and nutrition behaviours for children in this project. Within aspirations, parents desired for children to ‘keep their teeth’ and avoid false teeth, have a positive appearance, and preserve self-esteem. Parental worries related to child pain, negative appearance, sugar consumption, poor community oral health and rotten teeth. A discussion of findings results in the following recommendations: (1) consideration of the whole self, including mental health, in future oral health programming and research; (2) implementation of community-wide oral health programming, beyond parent-child dyads; and (3) prioritisation of community knowledge and traditions in oral health programming.
Moving towards the Future of Radio-Immunotherapy: Could We “Tailor” the Abscopal Effect on Head and Neck Cancer Patients?
Authors: De Felice Marco , Tammaro Mariagrazia , Leopardo Davide , Ianniello Giovanni Pietro , Turitto Giacinto
Published: November 2021

The abscopal effect (AbE) is defined as radiation-induced shrinkage of distant, non-treated, neoplastic lesions and it is considered the best clinical picture of the efficient immune stimulation by irradiation. The first report about abscopal tumor regression upon radiotherapy dates back to the beginning of the 20th century. The growing preclinical and clinical synergism between radiation and immunotherapy gave birth the purpose to more easily reproduce the abscopal effect, nevertheless, it is still rare in clinical practice. In this review we summarize immunological modulation of radiotherapy, focusing on the well-balanced equilibrium of tumor microenvironment and how radio-immunotherapy combinations can perturb it, with particular attention on head and neck squamous cell cancer. Finally, we investigate future perspectives, with the aim to “tailor” the abscopal effect to the patient.
Morphological Adaptation of Cave-Dwelling Ground Beetles in China Revealed by Geometric Morphometry (Coleoptera, Carabidae, Trechini)
Authors: Chen Mengzhen , Guo Wanru , Huang Sunbin , Luo Xiaozhu , Tian Mingyi , Liu Weixin
Published: November 2021

Cave-dwelling ground beetles in China represent the most impressive specific diversity and morphological adaptations of the cavernicolous ground beetles in the world, but they have not been systematically examined in quantitative terms. The present study focuses on the application of geometric morphological methods to address the morphological adaptations of the tribe Trechini, the most representative group in China. We have employed a geometric morphometry analysis of the head, pronotum, and elytra of 53 genera of Trechini, including 132 hypogean and 8 epigean species. Our results showed that the overall morphological variation of cave carabids has gradually specialized from an anophthalmic to semi-aphaenopsian to aphaenopsian type. There were extremely significant differences (p < 0.01) among four different adaptive types including aphaenopsian, semi-aphaenopsian, anophthalmic, and surface-dwelling Trechini when their adaptability to a cave environment was used as the basis for grouping. Furthermore, there were differences in the phenotypic tree of the head, pronotum, and elytra, and an integrated morphology. To the best of our knowledge, this is the first report on the analysis of the head, pronotum, and elytra of four different adaptive types of ground beetles in order to clarify the morphological adaptations of cavernicolous carabids to the cave environment.
Design of Low Cost Carbon Fiber Composites via Examining the Micromechanical Stress Distributions in A42 Bean-Shaped versus T650 Circular Fibers
Authors: Hanhan Imad , Sangid Michael D.
Published: November 2021

Recent advancements have led to new polyacrylonitrile carbon fiber precursors which reduce production costs, yet lead to bean-shaped cross-sections. While these bean-shaped fibers have comparable stiffness and ultimate strength values to typical carbon fibers, their unique morphology results in varying in-plane orientations and different microstructural stress distributions under loading, which are not well understood and can limit failure strength under complex loading scenarios. Therefore, this work used finite element simulations to compare longitudinal stress distributions in A42 (bean-shaped) and T650 (circular) carbon fiber composite microstructures. Specifically, a microscopy image of an A42/P6300 microstructure was processed to instantiate a 3D model, while a Monte Carlo approach (which accounts for size and in-plane orientation distributions) was used to create statistically equivalent A42/P6300 and T650/P6300 microstructures. First, the results showed that the measured in-plane orientations of the A42 carbon fibers for the analyzed specimen had an orderly distribution with peaks at |ϕ|=0∘,180∘. Additionally, the results showed that under 1.5% elongation, the A42/P6300 microstructure reached simulated failure at approximately 2108 MPa, while the T650/P6300 microstructure did not reach failure. A single fiber model showed that this was due to the curvature of A42 fibers which was 3.18 μm−1 higher at the inner corner, yielding a matrix stress that was 7 MPa higher compared to the T650/P6300 microstructure. Overall, this analysis is valuable to engineers designing new components using lower cost carbon fiber composites, based on the micromechanical stress distributions and unique packing abilities resulting from the A42 fiber morphologies.
The Role of Linguistic Typology, Target Language Proficiency and Prototypes in Learning Aspectual Contrasts in Italian as Additional Language
Authors: Vallerossa Francesco
Published: November 2021

The study examines how prototypes and typological relationships between the L1, the L2 and the target language (TL) interact with TL proficiency in learning Italian as additional language. Low-proficiency and high-proficiency undergraduate learners of Italian (N = 25) with Swedish as L1 performed an oral retelling story test, aiming to elicit the Italian aspectual contrast perfective-imperfective. Their tense selection was analyzed considering the predicates’ lexical aspect and the learners’ knowledge of a Romance L2, or lack thereof. The findings show that the typological proximity between the L2 and the TL exerts a differential role depending on TL proficiency. Initially, it is beneficial for accelerating the overall emergence of the imperfetto as an aspectual marker. However, the prototype factor and, more specifically, the predicates’ dynamicity influences the selection of past inflectional morphology. At more advanced stages, knowledge of a Romance language helps learners move beyond prototypical associations with the passato prossimo, but it does not seem to influence the use of the imperfetto among high-proficiency learners. These results are discussed in the light of research on the second and additional language learning of aspectual contrasts in Romance languages.
Fructose Consumption and Hepatocellular Carcinoma Promotion
Authors: Chávez-Rodríguez Lisette , Escobedo-Calvario Alejandro , Salas-Silva Soraya , Miranda-Labra Roxana U. , Bucio Leticia , Souza Verónica , Gutiérrez-Ruiz María Concepción , Gomez-Quiroz Luis E.
Published: November 2021

Hepatocellular carcinoma (HCC) accounts for 85% of primary liver cancer, the third most common cause of cancer-related deaths worldwide. Its incidence has been increasing in both men and women. In Western countries, high-calorie diets, mainly rich in carbohydrates such as fructose, represent a significant concern due to their repercussions on the population’s health. A high-fructose diet is related to the development of Metabolic-Associated Fatty Liver Disease (MAFLD), formerly named Non-Alcoholic Fatty Liver Disease (NAFLD), and the progression of HCC as it potentiates the lipogenic pathway and the accumulation of lipids. However, fructose metabolism seems to be different between the stages of the disease, carrying out a metabolic reprogramming to favor the proliferation, inflammation, and metastatic properties of cancer cells in HCC. This review focuses on a better understanding of fructose metabolism in both scenarios: MAFLD and HCC.
Source for In Situ Positron Annihilation Spectroscopy of Thermal—And Hydrogen-Induced Defects Based on the Cu-64 Isotope
Authors: Bordulev Iurii , Laptev Roman , Kabanov Denis , Ushakov Ivan , Kudiiarov Viktor , Lider Andrey
Published: November 2021

This work aims to investigate the 64Cu isotope applicability for positron annihilation experiments in in situ mode. We determined appropriate characteristics of this isotope for defect studies and implemented them under aggressive conditions (i.e., elevated temperature, hydrogen environment) in situ to determine the sensitivity of this approach to thermal vacancies and hydrogen-induced defects investigation. Titanium samples were used as test materials. The source was obtained by the activation of copper foil in the thermal neutron flux of a research nuclear reactor. Main spectrometric characteristics (e.g., the total number of counts, fraction of good signals, peak-to-noise ratio) of this source, as well as line-shaped parameters of the Doppler broadening spectrum (DBS), were studied experimentally. These characteristics for 64Cu (in contrast to positron sources with longer half-life) were shown to vary strongly with time, owing to the rapidly changing activity. These changes are predictable and should be considered in the analysis of experimental data to reveal information about the defect structure. The investigation of samples with a controlled density of defects revealed the suitability of 64Cu positron source with an activity of 2–40 MBq for defects studies by DBS. However, greater isotope activity could also be applied. The results of testing this source at high temperatures and in hydrogen atmosphere showed its suitability to thermal vacancies and hydrogen-induced defects studies in situ. The greatest changes in the defect structure of titanium alloy during high-temperature hydrogen saturation occurred at the cooling stage, when the formation of hydrides began, and were associated with an increase in the dislocation density.
Effects of High-Frequency (HF) Repetitive Transcranial Magnetic Stimulation (rTMS) on Upper Extremity Motor Function in Stroke Patients: A Systematic Review
Authors: Vabalaite Birute , Petruseviciene Laura , Savickas Raimondas , Kubilius Raimondas , Ignatavicius Povilas , Lendraitiene Egle
Published: November 2021

Background and Objectives: Repetitive transcranial magnetic stimulation (rTMS) is being widely used for treating upper extremity paresis after stroke, however, evidence of applying high-frequency rTMS (HF-rTMS) on the ipsilesional hemisphere for upper extremity motor recovery remains limited. This systematic review aimed to investigate the effect of high-frequency repetitive transcranial magnetic stimulation for upper extremity motor function recovery after a first-time ischaemic stroke. Materials and Methods: This systematic review was prepared according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. A comprehensive literature search was performed to identify all studies published before 12 February 2021. The search was performed on the following databases: PubMed, Ovid, The Cochrane Library. Results: A total of 6440 studies were found in the databases and four trials were included in the review. Three of the studies were randomized control trials (RCT), and one was a pseudo-RCT. Three of the studies showed good methodological quality and one study was rated as excellent. Fugl-Meyer Assessment (FMA) was performed in three out of four studies and the score significantly increased in the HF-rTMS treatment group compared with sham stimulation in all trials. Other measures used in the studies were handgrip strength, shoulder abduction, Motricity Index, Wolf Motor Function Test (WMFT), and Box and Block, although these tests did not show unanimous results. Overall, all four studies conveyed significantly better results in at least one test that was performed for hand motor function evaluation in a 10 Hz stimulation group while none of the tests showed any advantage for sham stimulation groups. Two studies reported headache as an adverse event (six patients in total). Conclusion: The overall results showed that HF-rTMS may increase impaired upper extremity motor function better than sham stimulation in stroke patients.
Pilot Plant for the Capture of Ammonia from the Atmosphere of Pig and Poultry Farms Using Gas-Permeable Membrane Technology
Authors: Soto-Herranz María , Sánchez-Báscones Mercedes , Antolín-Rodríguez Juan Manuel , Martín-Ramos Pablo
Published: November 2021

Gas-permeable membrane (GPM) technology is a possible solution to reduce ammonia (NH3) emissions from livestock housing. This paper presents the results obtained with an NH3-capture prototype based on the use of expanded polytetrafluoroethylene (ePTFE) membranes in real conditions in a gestating sow house and a free-range laying hen house, comparing them with the results obtained in controlled laboratory conditions for the same type of waste. The NH3 present in the air of the livestock housing was captured by reaction with an acidic solution flowing inside the membranes. The periods of continuous operation of the pilot plant were 232 days at the pig farm and 256 days at the poultry farm. The NH3 recovery rate at the end of those periods was 2.3 and 0.4 g TAN·m−2·d−1 in the pig and the poultry farms, respectively. The limiting factor for the capture process was the NH3 concentration in the air, with the highest recovery occurring in the most concentrated atmosphere. Differences in NH3 capture were observed between seasons and farms, with capture efficiencies of 1.62 and 0.33 g·m−2·d−1 in summer and 3.85 and 1.20 g·m−2·d−1 in winter for pig and poultry farms, respectively. The observed differences were mainly due to the higher ventilation frequency in the summer months, which resulted in a lower NH3 concentration inside the houses compared to the winter months. This is especially important when considering the real applicability of this technology. The results obtained suggest that GPM technology holds promise for limiting NH3 emissions from livestock housing with NH3 ambient concentrations close to 20 ppm or as part of manure storage facilities, given that it allows for recovery of nitrogen in a stable and concentrated solution, which can be used as a fertilizer.
Hepatic Response of Magnesium-Restricted Wild Type Mice
Authors: Fengler Vera H. , Macheiner Tanja , Goessler Walter , Ratzer Maria , Haybaeck Johannes , Sargsyan Karine
Published: November 2021

Magnesium-deficiency is implicated in many metabolic disorders, e.g., type 2 diabetes and metabolic syndrome, representing risk factors for non-alcoholic fatty liver disease (NAFLD). This study aims to investigate the contribution of magnesium-restriction to the development of NAFLD. Magnesium-deficiency was induced in C57BL/6 mice by feeding a magnesium-deficient-diet. Metabolic markers as well as markers of inflammation and liver function were assessed. Furthermore, liver tissue was examined histopathologically and compared with specimens from high-fat-diet fed and control mice. Finally, the hepatic inflammatory response was quantified by determining hepatic IL-6, TNFα, and MCP-1. Magnesium-restriction resulted in at least a 2-fold significant reduction of serum magnesium levels compared to the high-fat-diet fed and control mice, whereas the hepatic magnesium content was decreased due to high-fat-diet feeding. No changes in metabolic markers in magnesium-restricted mice were observed, while the cholesterol content was elevated in high-fat-diet fed mice. Magnesium-restricted mice additionally featured inflammation and enlarged hepatocytes in liver histology. Furthermore, magnesium-restricted and high-fat-diet fed mice exhibited elevated hepatic TNFα levels compared to control mice. Accordingly, our data suggest that magnesium is involved in hepatic inflammatory processes and hepatocyte enlargement, key histological features of human NAFLD, and may therefore contribute to development and progression of the disease.
A Failure Modes and Effects Analysis Framework for Assessing Geotechnical Risks of Tailings Dam Closure
Authors: Schafer Haley L. , Beier Nicholas A. , Macciotta Renato
Published: November 2021

Tailings dams remain on site following mine closures and must be designed and reclaimed to meet long-term goals, which may include walk-away closure or long-term care and maintenance. The underperformance of these structures can result in significant risks to public and environmental safety, as well as impacts on the future land use and economic activities near the structure. In Alberta, Canada, the expectation is for a tailings dam to be reclaimed and closed so that it can undergo deregistration. To aid in assessing the risks of underperformance during and after closure, a Generalized Failure Modes and Effects Analysis (G-FMEA) framework was developed to assess the long-term geotechnical risks for tailings dams in Alberta, with the goal of assessing the potential success of a tailings dam closure strategy. The G-FMEA is part of an initiative to enhance closure evaluations in Alberta in a collaborative effort between industry, the regulator, and academia. The G-FMEA incorporates the element of time to account for the evolution of the system, which should be applied at the planning stage and updated continually throughout the life of the facility. This paper presents the developed G-FMEA framework for tailings dams in Alberta, including the developed risk matrix framework.
Experimental Advances in Nanoparticle-Driven Stabilization of Liquid-Crystalline Blue Phases and Twist-Grain Boundary Phases
Authors: Cordoyiannis George , Lavrič Marta , Tzitzios Vasileios , Trček Maja , Lelidis Ioannis , Nounesis George , Kralj Samo , Thoen Jan , Kutnjak Zdravko
Published: November 2021

Recent advances in experimental studies of nanoparticle-driven stabilization of chiral liquid-crystalline phases are highlighted. The stabilization is achieved via the nanoparticles’ assembly in the defect lattices of the soft liquid-crystalline hosts. This is of significant importance for understanding the interactions of nanoparticles with topological defects and for envisioned technological applications. We demonstrate that blue phases are stabilized and twist-grain boundary phases are induced by dispersing surface-functionalized CdSSe quantum dots, spherical Au nanoparticles, as well as MoS2 nanoplatelets and reduced-graphene oxide nanosheets in chiral liquid crystals. Phase diagrams are shown based on calorimetric and optical measurements. Our findings related to the role of the nanoparticle core composition, size, shape, and surface coating on the stabilization effect are presented, followed by an overview of and comparison with other related studies in the literature. Moreover, the key points of the underlying mechanisms are summarized and prospects in the field are briefly discussed.
Construction of Functional Materials in Various Material Forms from Cellulosic Cholesteric Liquid Crystals
Authors: Miyagi Kazuma , Teramoto Yoshikuni
Published: November 2021

Wide use of bio-based polymers could play a key role in facilitating a more sustainable society because such polymers are renewable and ecofriendly. Cellulose is a representative bio-based polymer and has been used in various materials. To further expand the application of cellulose, it is crucial to develop functional materials utilizing cellulosic physicochemical properties that are acknowledged but insufficiently applied. Cellulose derivatives and cellulose nanocrystals exhibit a cholesteric liquid crystal (ChLC) property based on rigidity and chirality, and this property is promising for constructing next-generation functional materials. The form of such materials is an important factor because material form is closely related with function. To date, researchers have reported cellulosic ChLC materials with a wide range of material forms—such as films, gels, mesoporous materials, and emulsions—for diverse functions. We first briefly review the fundamental aspects of cellulosic ChLCs. Then we comprehensively review research on cellulosic ChLC functional materials in terms of their material forms. Thus, this review provides insights into the creation of novel cellulosic ChLC functional materials based on material form designed toward the expanded application of cellulosics.
Iron in Hydroxyapatite: Interstitial or Substitution Sites?
Authors: Avakyan Leon , Paramonova Ekaterina , Bystrov Vladimir , Coutinho José , Gomes Sandrine , Renaudin Guillaume
Published: November 2021

Iron-doped hydroxyapatite (Fe-HAp) is regarded as a promising magnetic material with innate biocompatibility. Despite the many studies reported in the literature, a detailed theoretical description of Fe inclusions is still missing. There is even no consensual view on what kind of Fe defects take place in Fe-HAp—iron interstitial or calcium substitutions? In order to address these questions, we employ modern first-principles methodologies, including hybrid density functional theory, to find the geometry, electronic, magnetic and thermodynamic properties of iron impurities in Fe-HAp. We consider a total of 26 defect configurations, including substitutional (phosphorus and calcium sites) and interstitial defects. Formation energies are estimated considering the boundaries of chemical potentials in stable hydroxyapatite. We show that the most probable defect configurations are: Fe3+ and Fe2+ substitutions of Ca(I) and Ca(II) sites under Ca-poor conditions. Conversely, Fe interstitials near the edge of the hydroxyl channel are favored in Ca-rich material. Substitutional Fe on the P site is also a probable defect, and unlike the other forms of Fe, it adopts a low-spin state. The analysis of Fe K-XANES spectra available in the literature shows that Fe-HAp usually contains iron in different configurations.
Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
Authors: Rudi Ludmila , Zinicovscaia Inga , Cepoi Liliana , Chiriac Tatiana , Peshkova Alexandra , Cepoi Anastasia , Grozdov Dmitrii
Published: November 2021

The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8–20 nm can penetrate the blood–brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process.
Exploration of Copper Oxide Nanoneedle Electrosynthesis Applied in the Degradation of Methylene Blue
Authors: Oyarzún Diego P. , Tello Alejandra , Sánchez Julio , Boulett Andrés , Linarez Pérez Omar E. , Martin-Trasanco Rudy , Pizarro Guadalupe del C. , Flores Marcos , Zúñiga César
Published: November 2021

In this study, we report a low cost, fast and unexplored electrochemical synthesis strategy of copper oxide nanoneedles films as well as their morphological and chemical characterization. The nanostructured films were prepared using electrochemical anodization in alkaline electrolyte solutions of ethylene glycol, water and fluoride ions. The film morphology shows nanoneedle-shaped structures, with lengths up to 1–2 μm; meanwhile, high-resolution X-ray photoelectron spectroscopy (HRXPS) and spectroscopy Raman analyses indicate that a mixture of Cu(II) and Cu(I) oxides, or only Cu(I) oxide, is obtained as the percentage of water in the electrolyte solution decreases. A preliminary study was also carried out for the photocatalytic degradation of the methylene blue (MB) dye under irradiation with simulated sunlight in the presence of the nanoneedles obtained, presenting a maximum degradation value of 88% of MB and, thus, demonstrating the potential characteristics of the material investigated in the degradation of organic dyes.
Nonporous, Strong, Stretchable, and Transparent Electrospun Aromatic Polyurea Nanocomposites as Potential Anticorrosion Coating Films
Authors: Auckloo Sheik , Palaniandy Khanisya , Hung Yew , Lazzara Giuseppe , Chai Siang-Piao , Pasbakhsh Pooria
Published: November 2021

This study, for the first time, focused on the fabrication of nonporous polyurea thin films (~200 microns) using the electrospinning method as a novel approach for coating applications. Multi-walled carbon nanotubes (MWCNTs) and hydrophilic-fumed nanosilica (HFNS) were added separately into electrospun polyurea films as nano-reinforcing fillers for the enhancement of properties. Neat polyurea films demonstrated a tensile strength of 14 MPa with an elongation of 360%. At a loading of 0.2% of MWCNTs, the highest tensile strength of 21 MPa and elongation of 402% were obtained, while the water contact angle remained almost unchanged (89°). Surface morphology analysis indicated that the production of polyurea fibers during electrospinning bonded together upon curing, leading to a nonporous film. Neat polyurea exhibited high thermal resistance with a degradation temperature of 380 °C. Upon reinforcement with 0.2% of MWCNTs and 0.4% of HFNS, it increased by ~7 °C. The storage modulus increased by 42 MPa with the addition of 0.2% of MWCNTs, implying a superior viscoelasticity of polyurea nanocomposite films. The results were benchmarked with anti-corrosive polymer coatings from the literature, revealing that the production of nonporous polyurea coatings with robust strength, elasticity, and thermal properties was achieved. Electrospun polyurea coatings are promising candidates as flexible anti-corrosive coatings for heat exchanges and electrical wires.
Progress toward SHAPE Constrained Computational Prediction of Tertiary Interactions in RNA Structure
Authors: De Bisschop Grégoire , Allouche Delphine , Frezza Elisa , Masquida Benoît , Ponty Yann , Will Sebastian , Sargueil Bruno
Published: November 2021

As more sequencing data accumulate and novel puzzling genetic regulations are discovered, the need for accurate automated modeling of RNA structure increases. RNA structure modeling from chemical probing experiments has made tremendous progress, however accurately predicting large RNA structures is still challenging for several reasons: RNA are inherently flexible and often adopt many energetically similar structures, which are not reliably distinguished by the available, incomplete thermodynamic model. Moreover, computationally, the problem is aggravated by the relevance of pseudoknots and non-canonical base pairs, which are hardly predicted efficiently. To identify nucleotides involved in pseudoknots and non-canonical interactions, we scrutinized the SHAPE reactivity of each nucleotide of the 188 nt long lariat-capping ribozyme under multiple conditions. Reactivities analyzed in the light of the X-ray structure were shown to report accurately the nucleotide status. Those that seemed paradoxical were rationalized by the nucleotide behavior along molecular dynamic simulations. We show that valuable information on intricate interactions can be deduced from probing with different reagents, and in the presence or absence of Mg2+. Furthermore, probing at increasing temperature was remarkably efficient at pointing to non-canonical interactions and pseudoknot pairings. The possibilities of following such strategies to inform structure modeling software are discussed.
The Influence of Burst-Firing EMF on Forskolin-Induced Pheochromocytoma (PC12) Plasma Membrane Extensions
Authors: Carniello Trevor N. , Lafrenie Robert M. , Dotta Blake T.
Published: November 2021

Previous research has demonstrated that pheochromocytoma (PC12) cells treated with forskolin provides a model for the in vitro examination of neuritogenesis. Exposure to electromagnetic fields (EMFs), especially those which have been designed to mimic biological function, can influence the functions of various biological systems. We aimed to assess whether exposure of PC12 cells treated with forskolin to patterned EMF would produce more plasma membrane extensions (PME) as compared to PC12 cells treated with forskolin alone (i.e., no EMF exposure). In addition, we aimed to determine whether the differences observed between the proportion of PME of PC12 cells treated with forskolin and exposed to EMF were specific to the intensity, pattern, or timing of the applied EMF. Our results showed an overall increase in PME for PC12 cells treated with forskolin and exposed to Burst-firing EMF as compared to PC12 cells receiving forskolin alone. No other patterned EMF investigated were deemed to be effective. Furthermore, intensity and timing of the Burst-firing pattern did not significantly alter the proportion of PME of PC12 cells treated with forskolin and exposed to patterned EMF.
Parenting Fears and Concerns during Pregnancy: A Qualitative Survey
Authors: Wilska Alisa , Rantanen Anja , Botha Elina , Joronen Katja
Published: November 2021

Previous research on the fears and anxieties of expectant mothers has focused mostly on their fears about giving birth rather than parenting. This study aims to describe mothers’ fears and concerns about parenthood during pregnancy and to examine the similarities and differences in the perspectives of primiparous and multiparous mothers. The qualitative research for this study was conducted in three postpartum units in Finland and focused on the responses to an open-ended question about parenting fears and concerns that was part of a questionnaire given to 250 mothers after they had given birth. The responses from the 128 mothers who answered this question were subject to inductive content analysis. Fears and concerns on parenthood included worries about coping with the future and everyday life with their new baby, the psychological burden of parenthood, their maternal resources and self-efficacy, meeting their baby’s needs, their baby’s health, concerns about their relationship with their partner and financial issues. Primiparous and multiparous mothers shared many of the same concerns, but some differences emerged. The findings contribute an interesting perspective to the social debate about declining birth rates and their psychosocial causes. Further studies are needed to examine the fears and concerns of younger adults, and even teens, about parenthood.
Trypanosomatid Richness in Wild and Synanthropic Small Mammals from a Biological Station in Rio de Janeiro, Brazil
Authors: Berbigier Alice Pereira , Barros Juliana Helena da Silva , Pontes Edilene Sousa , Lisboa Cristiane Varella , Gentile Rosana , Xavier Samanta Cristina das Chagas , Jansen Ana Maria , Roque André Luiz Rodrigues
Published: November 2021

Trypanosomatids are diverse and can infect several host species, including small mammals (rodents and marsupials). Between 2012 and 2014, 91 small mammals were surveyed for trypanosomatid infection in the Estação Biológica FIOCRUZ Mata Atlântica (EFMA), an Atlantic Forest area in Rio de Janeiro that presents different levels of conserved and degraded areas. Blood, skin, liver, and spleen samples were submitted to parasitological, serological, and molecular assays to detect the infection and determine the taxonomic status of their parasites. Sixty-eight individuals (74.7%; n = 91) were infected by trypanosomatids, including fourteen mixed infected by different trypanosomatid parasites. These hosts were infected by: T. cruzi DTU TcI (n = 12), T. cruzi DTU TcIV (n = 2), T. janseni (n = 15), T. dionisii (n = 1), and T. rangeli A (n = 1) detected in blood or tissue cultures, in addition to T. cruzi DTU TcI (n = 9) and Leishmania sp. (n = 1) only by the molecular diagnosis. Serological diagnosis was positive in 38 (71.6%) individuals for T. cruzi, the same amount for Leishmania spp., and 23 (43.3%) individuals were mixed infected. These data indicate a remarkable richness of trypanosomatid species/genotypes infecting small mammals, even in a disturbed area with low mammal species diversity—as is the case of the EFMA—reinforcing the generalist aspect of these parasites.
In Vivo Biodistribution, Clearance, and Biocompatibility of Multiple Carbon Dots Containing Nanoparticles for Biomedical Application
Authors: Liao Jinfeng , Yao Yuan , Lee Cheng-Hao , Wu Yongzhi , Li Pei
Published: November 2021

Current research on the use of carbon dots for various biological systems mainly focuses on the single carbon dots, while particles that contain multiple carbon dots have scarcely been investigated. Here, we assessed multiple carbon dots-crosslinked polyethyleneimine nanoparticles (CDs@PEI) for their in vivo biodistribution, clearance, biocompatibility, and cellular uptake. The in vivo studies demonstrate three unique features of the CDs@PEI nanoparticles: (1) the nanoparticles possess tumor-targeting ability with steady and prolonged retention time in the tumor region. (2) The nanoparticles show hepatobiliary excretion and are clear from the intestine in feces. (3) The nanoparticles have much better biocompatibility than the polyethyleneimine passivated single carbon dots (PEI-CD). We also found that pegylated CDs@PEI nanoparticles can be effectively taken up by the cells, which the confocal laser scanning microscope can image under different excitation wavelengths (at 405, 488, and 800 nm). These prior studies provide invaluable information and new opportunities for this new type of intrinsic photoluminescence nanoparticles in carbon dot-based biomedical applications.
Natural Carbon Nanodots: Toxicity Assessment and Theranostic Biological Application
Authors: Chan Ming-Hsien , Chen Bo-Gu , Ngo Loan Thi , Huang Wen-Tse , Li Chien-Hsiu , Liu Ru-Shi , Hsiao Michael
Published: November 2021

This review outlines the methods for preparing carbon dots (CDs) from various natural resources to select the process to produce CDs with the best biological application efficacy. The oxidative activity of CDs mainly involves photo-induced cell damage and the destruction of biofilm matrices through the production of reactive oxygen species (ROS), thereby causing cell auto-apoptosis. Recent research has found that CDs derived from organic carbon sources can treat cancer cells as effectively as conventional drugs without causing damage to normal cells. CDs obtained by heating a natural carbon source inherit properties similar to the carbon source from which they are derived. Importantly, these characteristics can be exploited to perform non-invasive targeted therapy on human cancers, avoiding the harm caused to the human body by conventional treatments. CDs are attractive for large-scale clinical applications. Water, herbs, plants, and probiotics are ideal carbon-containing sources that can be used to synthesize therapeutic and diagnostic CDs that have become the focus of attention due to their excellent light stability, fluorescence, good biocompatibility, and low toxicity. They can be applied as biosensors, bioimaging, diagnosis, and treatment applications. These advantages make CDs attractive for large-scale clinical application, providing new technologies and methods for disease occurrence, diagnosis, and treatment research.
Physico-Chemical, In Vitro and Ex Vivo Characterization of Meloxicam Potassium-Cyclodextrin Nanospheres
Authors: Varga Patrícia , Ambrus Rita , Szabó-Révész Piroska , Kókai Dávid , Burián Katalin , Bella Zsolt , Fenyvesi Ferenc , Bartos Csilla
Published: November 2021

Nasal drug delivery has many beneficial properties, such as avoiding the first pass metabolism and rapid onset of action. However, the limited residence time on the mucosa and limited absorption of certain molecules make the use of various excipients necessary to achieve high bioavailability. The application of mucoadhesive polymers can increase the contact time with the nasal mucosa, and permeation enhancers can enhance the absorption of the drug. We aimed to produce nanoparticles containing meloxicam potassium (MEL-P) by spray drying intended for nasal application. Various cyclodextrins (hydroxypropyl-β-cyclodextrin, α-cyclodextrin) and biocompatible polymers (hyaluronic acid, poly(vinylalcohol)) were used as excipients to increase the permeation of the drug and to prepare mucoadhesive products. Physico-chemical, in vitro and ex vivo biopharmaceutical characterization of the formulations were performed. As a result of spray drying, mucoadhesive nanospheres (average particle size <1 µm) were prepared which contained amorphous MEL-P. Cyclodextrin-MEL-P complexes were formed and the applied excipients increased the in vitro and ex vivo permeability of MEL-P. The highest amount of MEL-P permeated from the α-cyclodextrin-based poly(vinylalcohol)-containing samples in vitro (209 μg/cm2) and ex vivo (1.47 μg/mm2) as well. After further optimization, the resulting formulations may be promising for eliciting a rapid analgesic effect through the nasal route.
Synthesis, Characterisation and Antibacterial Properties of Silicone–Silver Thin Film for the Potential of Medical Device Applications
Authors: Aizamddin Muhammad Faiz , Mahat Mohd Muzamir , Zainal Ariffin Zaidah Zainal , Samsudin Irwan , Razali Muhammad Syafiek Mohd , Amir Muhammad ‘Abid
Published: November 2021

Silver (Ag) particles have sparked considerable interest in industry and academia, particularly for health and medical applications. Here, we present the “green” and simple synthesis of an Ag particle-based silicone (Si) thin film for medical device applications. Drop-casting and peel-off techniques were used to create an Si thin film containing 10–50% (v/v) of Ag particles. Electro impedance spectroscopy (EIS), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and tensile tests were used to demonstrate the electrical conductivity, crystallinity, morphology-elemental, and mechanical properties, respectively. The oriented crystalline structure and excellent electronic migration explained the highest conductivity value (1.40 × 10−5 S cm−1) of the 50% Ag–Si thin film. The findings regarding the evolution of the conductive network were supported by the diameter and distribution of Ag particles in the Si film. However, the larger size of the Ag particles in the Si film resulted in a lower tensile stress of 68.23% and an elongation rate of 68.25% compared to the pristine Si film. The antibacterial activity of the Ag–Si film against methicillin-resistant Staphylococcus aureus (MRSA), Bacillus cereus (B. cereus), Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa (P. aeruginosa) was investigated. These findings support Si–Ag thin films’ ability to avoid infection in any medical device application.
Icephobic Performance of Combined Fluorine-Containing Composite Layers on Al-Mg-Mn–Si Alloy Surface
Authors: Egorkin Vladimir S. , Mashtalyar Dmitry V. , Gnedenkov Andrey S. , Filonina Valeriia S. , Vyaliy Igor E. , Nadaraia Konstantine V. , Imshinetskiy Igor M. , Belov Evgeny A. , Izotov Nikolaj V. , Sinebryukhov Sergey L. , Gnedenkov Sergey V.
Published: November 2021

This paper presents the results of an evaluation of anti-icing properties of samples obtained by plasma electrolytic oxidation (PEO) with a subsequent application of superdispersed polytetrafluoroethylene (SPTFE) and polyvinylidenefluoride (PVDF). A combined treatment of the samples with SPTFE and PVDF is also presented. It is revealed that impregnation of a PEO layer with fluoropolymer materials leads to a significant increase in surface relief uniformity. Combined PVDF–SPFTE layers with a ratio of PVDF to SPTFE of 1:4 reveal the best electrochemical characteristics, hydrophobicity and icephobic properties among all of the studied samples. It is shown that the decrease in corrosion current density Ic for PVDF–SPFTE coatings is higher by more than five orders of magnitude in comparison with uncoated aluminum alloy. The contact angle for PVDF–SPFTE coatings attain 160.5°, which allows us to classify the coating as superhydrophobic with promising anti-icing performance. A treatment of a PEO layer with PVDF–SPFTE leads to a decrease in ice adhesion strength by 22.1 times compared to an untreated PEO coating.
Inhomogeneities in PNIPAM Aqueous Solutions: The Inside View by Spin Probe EPR Spectroscopy
Authors: Zubanova Ekaterina M. , Kostjuk Sergei V. , Timashev Peter S. , Rochev Yury A. , Kokorin Alexander I. , Melnikov Mikhail Ya. , Golubeva Elena N.
Published: November 2021

Coil to globule transition in poly(N-isopropylacrylamide) aqueous solutions was studied using spin probe continuous-wave electronic paramagnetic resonance (CW EPR) spectroscopy with an amphiphilic TEMPO radical as a guest molecule. Using Cu(II) ions as the “quencher” for fast-moving radicals in the liquid phase allowed obtaining the individual spectra of TEMPO radicals in polymer globule and observing inhomogeneities in solutions before globule collapsing. EPR spectra simulations confirm the formation of molten globules at the first step with further collapsing and water molecules coming out of the globule, making it denser.
Polyvinyl Alcohol Polymer Functionalized Graphene Oxide Decorated with Gadolinium Oxide for Sequestration of Radionuclides from Aqueous Medium: Characterization, Mechanism, and Environmental Feasibility Studies
Authors: Lingamdinne Lakshmi Prasanna , Koduru Janardhan Reddy , Chang Yoon-Young , Naushad Mu. , Yang Jae-Kyu
Published: November 2021

Uranium (U(VI)) and thorium (Th(IV)) ions produced by the nuclear and mining industries cause water pollution, thereby harming the environment and human health. In this study, gadolinium oxide-decorated polyvinyl alcohol-graphene oxide composite (PGO–Gd) was developed using a simple hydrothermal process to treat U(VI) and Th(IV) ions in water. The developed material was structurally characterized by highly advanced spectroscopy and microscopy techniques. The effects of pH, equilibration time and temperature on both radionuclides (U(VI) and Th(IV)) adsorption by PGO–Gd were examined. The PGO–Gd composite adsorbed both metal ions satisfactorily, with adsorption capacities of 427.50 and 455.0 mg g−1 at pH 4.0, respectively. The adsorption properties of both metal ions were found to be compatible with the Langmuir and pseudo–second-order kinetic models. Additionally, based on the thermodynamic characteristics, the adsorption was endothermic and spontaneous. Furthermore, the environmental viability of PGO–Gd and its application was demonstrated by studying its reusability in treating spiked surface water. PGO–Gd shows promise as an adsorbent in effectively removing both radionuclides from aqueous solutions.
Effects of Water and Alkaline Solution on Durability of Carbon-Glass Hybrid Fiber Reinforced Polymer Bars
Authors: Yu Yixun , Pan Yunfeng , Zhou Ronggui , Miao Xinbo
Published: November 2021

The glass fiber reacts with the hydroxyl owing to the concrete pore solution. A thin coat of carbon fiber wraps around the internal GFRP bars to improve the durability of internal GFRP bars in harsh environments. This paper investigates the effect of a thin carbon fiber coat on the durability of the carbon–glass hybrid fiber reinforced polymer bars (HFRP bars) in water, and compares the performance of FRP bars in alkaline solution. To this end, the water absorption behavior, interlaminar shear strength of both the GFRP bars and the HFRP bars was characterized in water and alkaline solution. The results indicate that the diffusivity coefficient of the carbon fiber coat is higher than that of internal GFRP in water. Compared to the GFRP bars in water, the HFRP bars have a higher diffusivity coefficient and saturation water absorption. It caused that the interlaminar shear strength of the HFRP bars aged in water at a temperature of 60 °C for 140 days decreases more markedly than that of the GFRP bars aged under similar conditions. Finally, it was proved that the thin carbon fiber coat does not slow the deterioration of the GFRP bars in water, while the carbon fiber coat significantly improves the retention of the interlaminar shear strength of the HFRP bars in the alkaline solution owing to the prevention of internal glass fiber reactivated by alkali ions.