bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamically electrical/thermal-tunable perfect absorber for a high-performance terahertz modulation
Authors: Zeng Dongwen , Zong Shu , Liu Guiqiang , Yuan Wen , Liu Xiaoshan , Liu Zhengqi
Published: October 2022

We present a high-performance functional perfect absorber in a wide range of terahertz (THz) wave based on a hybrid structure of graphene and vanadium dioxide (VO2) resonators. Dynamically electrical and thermal tunable absorption is achieved due to the management on the resonant properties via the external surroundings. Multifunctional manipulations can be further realized within such absorber platform. For instance, a wide-frequency terahertz perfect absorber with the operation frequency range covering from 1.594 THz to 3.272 THz can be realized when the conductivity of VO2 is set to 100000 S/m (metal phase) and the Fermi level of graphene is 0.01 eV. The absorption can be dynamically changed from 0 to 99.98% and in verse by adjusting the conductivity of VO2. The impedance matching theory is introduced to analyze and elucidate the wideband absorption rate. In addition, the absorber can be changed from wideband absorption to dual-band absorption by adjusting the Fermi level of graphene from 0.01 eV to 0.7 eV when the conductivity of VO2 is fixed at 100000 S/m. Besides, the analysis of the chiral characteristics of the helical structure shows that the extinction cross-section has a circular dichroic response under the excitation of two different circularly polarized lights (CPL). Our study proposes approaches to manipulate the wide-band terahertz wave with multiple ways, paving the way for the development of technologies in the fields of switches, modulators, and imaging devices.
Millimeter-scale ultrathin suspended metasurface integrated high-finesse optomechanical cavity
Authors: Xu Jipeng , Liu Ken , Sang You , Tan Zhongqi , Guo Chucai , Zhu Zhihong
Published: October 2022

A typical optomechanical system is a cavity with one movable mirror and one fixed mirror. However, this configuration has been considered incapable of integrating sensitive mechanical elements while maintaining high cavity finesse. Although the membrane-in-the-middle solution seems to be able to overcome this contradiction, it introduces additional components that will lead to unexpected insertion loss, resulting in reduced cavity quality. Here we propose a Fabry–Perot optomechanical cavity composed of an ultrathin suspended Si3N4 metasurface and a fixed Bragg grating mirror, with a measured finesse up to 1100. Transmission loss of this cavity is very low as the reflectivity of this suspended metasurface tends to unity around 1550 nm. Meanwhile, the metasurface has a millimeter-scale transverse dimension and a thickness of only 110 nm, which guarantees a sensitive mechanical response and low cavity diffraction loss. Our metasurface-based high-finesse optomechanical cavity has a compact structure, which facilitates the development of quantum and integrated optomechanical devices.
High-energy Q switched Yb-doped fiber laser based on a ternary layered structured Ti2AlC saturable absorber
Authors: Zhang Kang , Feng Ming , Xie Jinyue , Sang Xu , Sun Guoqing , Tian Ye , Song Feng , Huang Wei
Published: October 2022

Ti2AlC is a kind of ternary layered structured ceramic metal compound, combining the advantages of both ceramic and metal. Herein, the saturable absorption performance of Ti2AlC at the 1-µm wave band is investigated. The Ti2AlC behaves with excellent saturable absorption, which has a modulation depth of 14.53% and a saturable intensity of 13.27 MW/cm2. An all-normal dispersion fiber laser based on the Ti2AlC saturable absorber (SA) is constructed. The repetition frequency of the Q switched pulses increased from 44 to 49 kHz as the pump power rose from 276 to 365 mW, and the corresponding pulse width decreased from 3.64 to 2.42 µs. The maximum output single Q switched pulse energy is as high as 169.8 nJ. Our experiments prove that the MAX phase Ti2AlC has potential as a low-cost, simple preparation, and broadband SA material. To the best of our knowledge, this is the first demonstration of Ti2AlC serving as a SA material achieving Q switched operation at the 1-µm wave band.
Spatial models of pattern formation during phagocytosis
Authors: Herron John Cody , Hu Shiqiong , Liu Bei , Watanabe Takashi , Hahn Klaus M. , Elston Timothy C.
Published: October 2022

Phagocytosis, the biological process in which cells ingest large particles such as bacteria, is a key component of the innate immune response. Fcγ receptor (FcγR)-mediated phagocytosis is initiated when these receptors are activated after binding immunoglobulin G (IgG). Receptor activation initiates a signaling cascade that leads to the formation of the phagocytic cup and culminates with ingestion of the foreign particle. In the experimental system termed “frustrated phagocytosis”, cells attempt to internalize micropatterned disks of IgG. Cells that engage in frustrated phagocytosis form “rosettes” of actin-enriched structures called podosomes around the IgG disk. The mechanism that generates the rosette pattern is unknown. We present data that supports the involvement of Cdc42, a member of the Rho family of GTPases, in pattern formation. Cdc42 acts downstream of receptor activation, upstream of actin polymerization, and is known to play a role in polarity establishment. Reaction-diffusion models for GTPase spatiotemporal dynamics exist. We demonstrate how the addition of negative feedback and minor changes to these models can generate the experimentally observed rosette pattern of podosomes. We show that this pattern formation can occur through two general mechanisms. In the first mechanism, an intermediate species forms a ring of high activity around the IgG disk, which then promotes rosette organization. The second mechanism does not require initial ring formation but relies on spatial gradients of intermediate chemical species that are selectively activated over the IgG patch. Finally, we analyze the models to suggest experiments to test their validity.
A fibrin enhanced thrombosis model for medical devices operating at low shear regimes or large surface areas
Authors: Méndez Rojano Rodrigo , Lai Angela , Zhussupbekov Mansur , Burgreen Greg W. , Cook Keith , Antaki James F.
Published: October 2022

Over the past decade, much of the development of computational models of device-related thrombosis has focused on platelet activity. While those models have been successful in predicting thrombus formation in medical devices operating at high shear rates (> 5000 s−1), they cannot be directly applied to low-shear devices, such as blood oxygenators and catheters, where emerging information suggest that fibrin formation is the predominant mechanism of clotting and platelet activity plays a secondary role. In the current work, we augment an existing platelet-based model of thrombosis with a partial model of the coagulation cascade that includes contact activation of factor XII and fibrin production. To calibrate the model, we simulate a backward-facing-step flow channel that has been extensively characterized in-vitro. Next, we perform blood perfusion experiments through a microfluidic chamber mimicking a hollow fiber membrane oxygenator and validate the model against these observations. The simulation results closely match the time evolution of the thrombus height and length in the backward-facing-step experiment. Application of the model to the microfluidic hollow fiber bundle chamber capture both gross features such as the increasing clotting trend towards the outlet of the chamber, as well as finer local features such as the structure of fibrin around individual hollow fibers. Our results are in line with recent findings that suggest fibrin production, through contact activation of factor XII, drives the thrombus formation in medical devices operating at low shear rates with large surface area to volume ratios.
HAL-X: Scalable hierarchical clustering for rapid and tunable single-cell analysis
Authors: Anibal James , Day Alexandre G. , Bahadiroglu Erol , O’Neil Liam , Phan Long , Peltekian Alec , Erez Amir , Kaplan Mariana , Altan-Bonnet Grégoire , Mehta Pankaj
Published: October 2022

Data clustering plays a significant role in biomedical sciences, particularly in single-cell data analysis. Researchers use clustering algorithms to group individual cells into populations that can be evaluated across different levels of disease progression, drug response, and other clinical statuses. In many cases, multiple sets of clusters must be generated to assess varying levels of cluster specificity. For example, there are many subtypes of leukocytes (e.g. T cells), whose individual preponderance and phenotype must be assessed for statistical/functional significance. In this report, we introduce a novel hierarchical density clustering algorithm (HAL-x) that uses supervised linkage methods to build a cluster hierarchy on raw single-cell data. With this new approach, HAL-x can quickly predict multiple sets of labels for immense datasets, achieving a considerable improvement in computational efficiency on large datasets compared to existing methods. We also show that cell clusters generated by HAL-x yield near-perfect F1-scores when classifying different clinical statuses based on single-cell profiles. Our hierarchical density clustering algorithm achieves high accuracy in single cell classification in a scalable, tunable and rapid manner.
The burden of catastrophic and impoverishing health expenditure in Armenia: An analysis of Integrated Living Conditions Surveys, 2014–2018
Authors: Kazungu Jacob , Meyer Christina L. , Sargsyan Kristine Gallagher , Qaiser Seemi , Chukwuma Adanna
Published: October 2022

Armenia’s health spending is characterized by low public spending and high out-of-pocket expenditure (OOP), which not only poses a financial barrier to accessing healthcare for Armenians but can also impoverish them. We analyzed Armenia’s Integrated Living Conditions Surveys 2014–2018 data to assess the incidence and correlates of catastrophic health expenditure (CHE) and impoverishment. Households were considered to have incurred CHE if their annual OOP exceeded 40 percent of the per capita annual household non-food expenditure. We assessed impoverishment using the US$1.90 per person per-day international poverty line and the US$5.50 per person per-day upper-middle-income country poverty line. Logistic regression models were fitted to assess the correlates of CHE and impoverishment. We found that the incidence of CHE peaked in 2017 before declining in 2018. Impoverishment decreased until 2017 before rising in 2018. After adjusting for sociodemographic factors, households were more likely to incur CHE if the household head was older than 34 years, located in urban areas, had at least one disabled member, and had at least one member with hypertension. Households with at least one hypertensive member or who resided in urban areas were more likely to be impoverished due to OOP. Paid employment and high socioeconomic status were protective against both CHE and impoverishment from OOP. This detailed analysis offers a nuanced insight into the trends in Armenia’s financial risk protection against catastrophic and impoverishing health expenditures, and the groups predominantly affected. The incidence of CHE and impoverishment in Armenia remains high with a higher incidence among vulnerable groups, including those living with chronic disease, disability, and the unemployed. Armenia should consider different mechanisms such as subsidizing medication and hospitalization costs for the poorest to alleviate the burden of OOP.
Targeting Echinococcus multilocularis PIM kinase for improving anti-parasitic chemotherapy
Authors: Koike Akito , Becker Frank , Sennhenn Peter , Kim Jason , Zhang Jenny , Hannus Stefan , Brehm Klaus
Published: October 2022

Background

The potentially lethal zoonosis alveolar echinococcosis (AE) is caused by the metacestode larval stage of the tapeworm Echinococcus multilocularis. Current AE treatment options are limited and rely on surgery as well as on chemotherapy involving benzimidazoles (BZ). BZ treatment, however, is mostly parasitostatic only, must be given for prolonged time periods, and is associated with adverse side effects. Novel treatment options are thus urgently needed.

Methodology/principal findings

By applying a broad range of kinase inhibitors to E. multilocularis stem cell cultures we identified the proto-oncogene PIM kinase as a promising target for anti-AE chemotherapy. The gene encoding the respective E. multilocularis ortholog, EmPim, was characterized and in situ hybridization assays indicated its expression in parasite stem cells. By yeast two-hybrid assays we demonstrate interaction of EmPim with E. multilocularis CDC25, indicating an involvement of EmPim in parasite cell cycle regulation. Small molecule compounds SGI-1776 and CX-6258, originally found to effectively inhibit human PIM kinases, exhibited detrimental effects on in vitro cultured parasite metacestode vesicles and prevented the formation of mature vesicles from parasite stem cell cultures. To improve compound specificity for EmPim, we applied a high throughput in silico modelling approach, leading to the identification of compound Z196138710. When applied to in vitro cultured metacestode vesicles and parasite cell cultures, Z196138710 proved equally detrimental as SGI-1776 and CX-6258 but displayed significantly reduced toxicity towards human HEK293T and HepG2 cells.

Conclusions/significance

Repurposing of kinase inhibitors initially designed to affect mammalian kinases for helminth disease treatment is often hampered by adverse side effects of respective compounds on human cells. Here we demonstrate the utility of high throughput in silico approaches to design small molecule compounds of higher specificity for parasite cells. We propose EmPim as a promising target for respective approaches towards AE treatment.
Comparison of variable selection procedures and investigation of the role of shrinkage in linear regression-protocol of a simulation study in low-dimensional data
Authors: Kipruto Edwin , Sauerbrei Willi
Published: October 2022

In low-dimensional data and within the framework of a classical linear regression model, we intend to compare variable selection methods and investigate the role of shrinkage of regression estimates in a simulation study. Our primary aim is to build descriptive models that capture the data structure parsimoniously, while our secondary aim is to derive a prediction model. Simulation studies are an important tool in statistical methodology research if they are well designed, executed, and reported. However, bias in favor of an “own” preferred method is prevalent in most simulation studies in which a new method is proposed and compared with existing methods. To overcome such bias, neutral comparison studies, which disregard the superiority or inferiority of a particular method, have been proposed. In this paper, we designed a simulation study with key principles of neutral comparison studies in mind, though certain unintentional biases cannot be ruled out. To improve the design and reporting of a simulation study, we followed the recently proposed ADEMP structure, which entails defining the aims (A), data-generating mechanisms (D), estimand/target of analysis (E), methods (M), and performance measures (P). To ensure the reproducibility of results, we published the protocol before conducting the study. In addition, we presented earlier versions of the design to several experts whose feedback influenced certain aspects of the design. We will compare popular penalized regression methods (lasso, adaptive lasso, relaxed lasso, and nonnegative garrote) that combine variable selection and shrinkage with classical variable selection methods (best subset selection and backward elimination) with and without post-estimation shrinkage of parameter estimates.
Patient specific approach to analysis of shear-induced platelet activation in haemodialysis arteriovenous fistula
Authors: Salikhova Tatiana Yu , Pushin Denis M. , Nesterenko Igor V. , Biryukova Lyudmila S. , Guria Georgy Th
Published: October 2022

Shear-induced platelet activation (SIPAct) is an important mechanism of thrombosis initiation under high blood flow. This mechanism relies on the interaction of platelets with the von Willebrand factor (VWF) capable of unfolding under high shear stress. High shear stress occurs in the arteriovenous fistula (AVF) commonly used for haemodialysis. A novel patient-specific approach for the modelling of SIPAct in the AVF was proposed. This enabled us to estimate the SIPAct level via computational fluid dynamics. The suggested approach was applied for the SIPAct analysis in AVF geometries reconstructed from medical images. The approach facilitates the determination of the SIPAct level dependence on both biomechanical (AVF flow rate) and biochemical factors (VWF multimer size). It was found that the dependence of the SIPAct level on the AVF flow rate can be approximated by a power law. The critical flow rate was a decreasing function of the VWF multimer size. Moreover, the critical AVF flow rate highly depended on patient-specific factors, e.g., the vessel geometry. This indicates that the approach may be adopted to elucidate patient-specific thrombosis risk factors in haemodialysis patients.
Co-creating safe spaces: Study protocol for translational research on innovative alternatives to the emergency department for people experiencing emotional distress and/or suicidal crisis
Authors: Banfield Michelle , Fitzpatrick Scott J. , Lamb Heather , Giugni Melanie , Calear Alison L. , Stewart Erin , Pavloudis Maree , Ellen Lucy , Sargent Ginny , Skeat Helen , Edwards Bronwen , Miller Benn , Gulliver Amelia , Ellis Louise A. , Bliokas Vida , Goj Purity , Lee Melissa , Stewart Kelly , Webb Glenda , Main Merkitta , Lumby Carrie , Wells Kelly , McKay Carolyn , Batterham Philip J. , Morse Alyssa R. , Shand Fiona ,   Stride Safe Space Team
Published: October 2022

Introduction

Safe spaces are an alternative to emergency departments, which are often unable to provide optimum care for people experiencing emotional distress and/or suicidal crisis. At present, there are several different safe space models being trialled in Australia. However, research examining the effectiveness of safe space models, especially in community settings, is rare. In this paper, we present a protocol for a study in which we will investigate the implementation, effectiveness, and sustainability of safe space models as genuine alternatives for people who might usually present to the emergency department or choose not to access help due to past negative experiences.

Material and methods

We will use a mixed methods, co-designed study design, conducted according to the principles of community-based participatory research to obtain deep insights into the benefits of different safe space models, potential challenges, and facilitators of effective practice. We developed the study plan and evaluation framework using the RE-AIM framework, and this will be used to assess key outcomes related toreach,effectiveness,adoption,implementation, andmaintenance. Data collection will comprise quantitative measures on access, use, satisfaction, (cost) effectiveness, distress, and suicidal ideation; and qualitative assessments of service implementation, experience, feasibility, acceptability, community awareness, and the fidelity of the models to service co-design. Data will be collected and analysed concurrently throughout the trial period of the initiatives.

Discussion

This study will enable an extensive investigation of safe spaces that will inform local delivery and provide a broader understanding of the key features of safe spaces as acceptable and effective alternatives to hospital-based care for people experiencing emotional distress and/or suicidal crisis. This study will also contribute to a growing body of research on the role and benefits of peer support and provide critical new knowledge on the successes and challenges of service co-design to inform future practice.
The realized efficacy of indoor residual spraying campaigns falls quickly below the recommended WHO threshold when coverage, pace of spraying and residual efficacy on different wall types are considered
Authors: Fernández Montoya Lucia , Máquina Mara , Martí-Soler Helena , Sherrard-Smith Ellie , Alafo Celso , Opiyo Mercy , Comiche Kiba , Galatas Beatriz , Huijben Silvie , Koekemoer Lizette L. , Oliver Shüné V. , Maartens Francois , Marrenjo Dulcisaria , Cuamba Nelson , Aide Pedro , Saúte Francisco , Paaijmans Krijn P.
Published: October 2022

Indoor residual spraying (IRS) has been and remains an important malaria control intervention in southern Mozambique, South Africa and Eswatini. A better understanding of the effectiveness of IRS campaigns is critical to guide future elimination efforts. We analyze the three IRS campaigns conducted during a malaria elimination demonstration project in southern Mozambique, the “Magude project”, and propose a new method to calculate the efficacy of IRS campaigns adjusting for IRS coverage, pace of house spraying and IRS residual efficacy on different wall types. Anopheles funestus sensu lato (s.l.) and An. gambiae s.l. were susceptible to pirimiphos-methyl and DDT. Anopheles funestus s.l. was resistant to pyrethroids, with 24h post-exposure mortality being lower for An. funestus sensu stricto (s.s.) than for An. parensis (collected indoors). The percentage of structures sprayed was above 90% and percentage of people covered above 86% in all three IRS campaigns. The percentage of households sprayed was above 83% in 2015 and 2016, but not assessed in 2017. Mosquito mortality 24h post-exposure stayed above 80% for 196 days after the 2016 IRS campaign and 222 days after the 2017 campaign and was 1.5 months longer on mud walls than on cement walls. This was extended by up to two months when 120h post-exposure mortality was considered. The district-level realized IRS efficacy was 113 days after the 2016 campaign. While the coverage of IRS campaigns in Magude were high, IRS protection did not remain optimal for the entire high malaria transmissions season. The use of a longer-lasting IRS product could have further supported the interruption of malaria transmission in the district. To better estimate the protection afforded by IRS campaigns, National Malaria Control Programs and partners are encouraged to adjust the calculation of IRS efficacy for IRS coverage, pace of house spraying during the campaign and IRS efficacy on different wall types combined with wall type distribution in the sprayed area.
Seasonal influenza, its complications and related healthcare resource utilization among people 60 years and older: A descriptive retrospective study in Japan
Authors: Hagiwara Yuriko , Harada Kazumasa , Nealon Joshua , Okumura Yasuyuki , Kimura Takeshi , Chaves Sandra S.
Published: October 2022

Evidence suggests that older people aged ≥65 years and those aged 60–64 years with chronic medical conditions are at higher risk of developing severe complications due to influenza virus infection when compared with young, healthy adults. Although seasonal influenza is monitored through a nationwide passive surveillance in Japan, influenza related outcomes and medical resource consumption have not been fully documented. This retrospective database study aimed to describe the epidemiological and clinical characteristics of medically attended influenza cases aged ≥60 years and the associated medical resource consumption in Japan. We used clinically diagnosed influenza (CDI) based on the international classification of disease codes, and laboratory-confirmed influenza (LCI) based on influenza test results, to identify the patient population during a total of nine seasons (2010/2011 to 2018/2019). A total of 372,356 CDI and 31,122 LCI cases were identified from 77 medical institutions. The highest numbers of medically-attended influenza episodes were in patients aged 65–74 years and 75–84 years. On average, across seasons, 5.9% of all-cause hospitalizations were attributable to CDI and 0.4% were LCI. Influenza viruses type A and B co-circulated annually in varying degree of intensity and were associated with similar level of complications, including cardiovascular-related. Oxygen therapy increased with age; by contrast, mechanical ventilation, dialysis, blood transfusion, and intensive care unit admission were higher in the younger groups. In-hospital mortality for inpatients aged ≥ 85 years with CDI and LCI were 18.6% and 15.5%, respectively. Considering the burden associated with medically-attended influenza in this population, influenza prevention, laboratory confirmation and clinical management should be emphasized by general practicians and specialists like cardiologists to protect this aging population.
A Genome-Wide Association study in Arabidopsis thaliana to decipher the adaptive genetics of quantitative disease resistance in a native heterogeneous environment
Authors: Roux Fabrice , Frachon Léa
Published: October 2022

Pathogens are often the main selective agents acting in plant communities, thereby influencing the distribution of polymorphism at loci affecting resistance within and among natural plant populations. In addition, the outcome of plant-pathogen interactions can be drastically affected by abiotic and biotic factors at different spatial and temporal grains. The characterization of the adaptive genetic architecture of disease resistance in native heterogeneous environments is however still missing. In this study, we conducted anin situGenome-Wide Association study in the spatially heterogeneous native habitat of a highly genetically polymorphic local mapping population ofArabidopsis thaliana, to unravel the adaptive genetic architecture of quantitative disease resistance. Disease resistance largely differed among three native soils and was affected by the presence of the grassPoa annua. The observation of strong crossing reactions norms among the 195A.thalianagenotypes for disease resistance among micro-habitats, combined with a negative fecundity-disease resistance relationship in each micro-habitat, suggest that alternative local genotypes ofA.thalianaare favored under contrasting environmental conditions at the scale of few meters. A complex genetic architecture was detected for disease resistance and fecundity. However, only few QTLs were common between these two traits. Heterogeneous selection in this local population should therefore promote the maintenance of polymorphism at only few candidate resistance genes.
Demographic and epidemiological characteristics of pregnant and postpartum women who died from severe acute respiratory syndrome in Brazil: A retrospective cohort study comparing COVID-19 and nonspecific etiologic causes
Authors: Franco Veridiana Freire , Rodrigues Agatha Sacramento , Junior Elias Ribeiro Rosa , de Godói Luciana Graziela , Monroy Nátaly Adriana Jimenez , da Costa Rafaela Alkmin , Francisco Rossana Pulcineli Vieira
Published: October 2022

The objective of this study is to compare the demographic characteristics and symptoms in pregnant and postpartum women who died from Severe Acute Respiratory Syndrome (SARS) caused by COVID-19 or by nonspecific cause in different states of Brazil. This is a retrospective cohort study and the analysis was conducted on SARS death records between 02/16/2020 and 04/17/2021, obtained from the Information System for the Epidemiological Surveillance of Influenza (Sistema de Informação da Vigilância Epidemiológica da Gripe, SIVEP-Gripe). Pregnant and postpartum women, aged between 10 and 55 years, who died from SARS, were included and classified into two groups: SARS due to confirmed COVID-19 or SARS due to nonspecific cause. The cases were analyzed according to the women’s demographic and epidemiological characteristics, clinical symptoms, risk factors and disease evolution. As results, 19,333 pregnant and postpartum women were identified. From these, 1,279 died (1,026 deaths from COVID-19 and 253 deaths from SARS with nonspecific cause). The groups showed significant differences in age, education, race, and occurrence of obesity and chronic lung disease. The group of women who died from confirmed COVID-19 presented a significantly higher frequency of symptoms of fever, cough, fatigue, loss of taste, and loss of smell, as well as a higher rate of admission to the intensive care unit (ICU). Data analysis draws attention to the high number of cases of SARS without a causal diagnosis, the low access to ICU and orotracheal intubation (OTI), which might be explained by the demographic and regional inequalities in the access to healthcare.
Maternal weight status and the composition of the human milk microbiome: A scoping review
Authors: Daiy Katherine , Harries Victoria , Nyhan Kate , Marcinkowska Urszula M.
Published: October 2022

The human milk microbiome is thought to partly contribute to the assembly of the infant gut microbiome, a microbial community with important implications for infant health and development. While obesity has well-established links with the adult gut microbiome, less is known about how it affects the human milk microbiome. In this scoping review, we synthesize the current literature on the microbial composition of human milk by maternal weight status, defined broadly as BMI (prepregnancy and postpartum) and gestational weight gain (GWG). This study followed thea prioriprotocol published in Prospero (registration #: CRD42020165633). We searched the following databases for studies reporting maternal weight status and a characterization of milk microbiota through culture-dependent and culture-independent methods: MEDLINE, Embase, Web of Science, CINAHL, and Scopus. After screening 6,365 studies, we found 20 longitudinal and cross-sectional studies investigating associations between maternal weight status and the composition of the milk microbiome. While some studies reported no associations, many others reported that women with a pre-pregnancy or postpartum BMI characterized as overweight or obese, or with excessive GWG, had higher abundances of the genusStaphylococcus, lowerBifidobacteriumabundance, and lower alpha diversity (within-sample diversity). This review suggests that maternal weight status is minorly associated with the composition of the milk microbiome in various ways. We offer potential explanations for these findings, as well as suggestions for future research.
Solid matrix priming improves cauliflower and broccoli seed germination and early growth under the suboptimal temperature conditions
Authors: Ling-Yun Wu , Jun Yan , Zhi-wu Huang , Yan-Hui Wan , Wei-Min Zhu
Published: October 2022

Seed priming is an effective method for imparting stress tolerance to plants. This study aimed to analyze the effects of solid matrix priming (SMP) on cauliflower and broccoli seed germination and early seedling growth under suboptimal temperature conditions. The SMP method used in this study included the following steps: (1) mixing seeds with vermiculite and water at a ratio of 2:3:2.5 (w/w/v) and incubating for 2 days in the dark at 20°C; (2) drying the SM-primed seed; (3) germinating the SM-primed and the nonprimed seeds at 10, 15, 20, and 25°C; (4) analyzing the antioxidant enzyme activities of SM-primed and nonprimed germinating broccoli and cauliflower seeds in the early germination stage at 10, 15, 20, and 25°C; and (5) testing the emergence of SM-primed and nonprimed control seeds in the early spring glasshouse. The results showed that the SMP improved seed germination vigor and early seedling growth and increased the activities of peroxidase and ascorbate peroxidase in the germinating cauliflower and broccoli seeds under the suboptimal temperature conditions in the early germination stage compared with nonprimed seeds. It was observed that the suboptimal temperature conditions (i.e., 10 and 15°C) suppressed SM-primed and nonprimed seed germination and early seedling growth of cauliflower and broccoli. Inside a greenhouse, the SMP improved the emergence of cauliflower and broccoli seeds during the early spring season. SMP is an effective method for improving seed germination and the emergence of cauliflower and broccoli under suboptimal temperature conditions.
Differential expression patterns of genes associated with metabolisms, muscle growth and repair in Pectoralis major muscles of fast- and medium-growing chickens
Authors: Malila Yuwares , Thanatsang Krittaporn V. , Sanpinit Pornnicha , Arayamethakorn Sopacha , Soglia Francesca , Zappaterra Martina , Bordini Martina , Sirri Federico , Rungrassamee Wanilada , Davoli Roberta , Petracci Massimiliano
Published: October 2022

The aim of this study was to investigate the expression of genes related to muscle growth, hypoxia and oxidative stress responses, a multi-substrate serine/threonine-protein kinase (AMPK) and AMPK-related kinases, carbohydrate metabolism, satellite cells activities and fibro- adipogenic progenitors (FAPs) in fast-growing (FG) (n = 30) and medium-growing (MG) chickens (n = 30). Pectoralis major muscles were collected at 7d, 14d, 21d, 28d, 35d and 42d of age. According to their macroscopic features, the samples from FG up to 21d of age were classified as unaffected, while all samples collected at an older age exhibited macroscopic features ascribable to white striping and/or wooden breast abnormalities. In contrast, MG samples did not show any feature associated to muscle disorders. The absolute transcript abundance of 33 target genes was examined by droplet digital polymerase chain reaction. The results showed differential gene expression profiles between FG and MG chickens at different ages. While most genes remained unchanged in MG chickens, the expression patterns of several genes in FG were significantly affected by age. Genes encoding alpha 1, alpha 2, beta 2 and gamma 3 isoforms of AMPK, as well as AMPK-related kinases, were identified as differentially expressed between the two strains. The results support the hypothesis of oxidative stress-induced muscle damage with metabolic alterations in FG chickens. An increased expression of ANXA2, DES, LITAF, MMP14, MYF5 and TGFB1 was observed in FG strain. The results suggest the occurrence of dysregulation of FAP proliferation and differentiation occurring during muscle repair. FAPs could play an important role in defining the proliferation of connective tissue (fibrosis) and deposition of intermuscular adipose tissue which represents distinctive traits of muscle abnormalities. Overall, these findings demonstrate that dysregulated molecular processes associated with myopathic lesions in chickens are strongly influenced by growth rate, and, to some extent, by age.
Low-dose versus high-dose dexamethasone for hospitalized patients with COVID-19 pneumonia: A randomized clinical trial
Authors: Wu Huimin , Daouk Salim , Kebbe Jad , Chaudry Fawad , Harper Jarrod , Brown Brent
Published: October 2022

Background

Dexamethasone 6 mg daily for 10 days is the recommended treatment for patients with severe or critical coronavirus disease 2019 (COVID-19). The evidence on the benefit of high-dose dexamethasone is limited. The goal of this study was to assess the effects of 6 mg daily vs. 20 mg daily of dexamethasone in hospitalized patients with COVID-19 pneumonia.

Methods

We conducted a single-center, randomized, clinical trial involving hospitalized patients with COVID-19 pneumonia. Participants were randomized 1:1 to dexamethasone 6 mg daily or dexamethasone 20 mg daily, and were stratified by the WHO-Ordinal Scale for Clinical Improvement (OSCI). The primary outcome was clinical improvement equal to or greater than 2 points by OSCI on day 28. Secondary outcomes were 28-day mortality, intensive care unit-free days, and ventilator-free days on day 28.

Results

Of the 107 patients who enrolled and completed the follow up, 55 patients enrolled in the low-dose group and 52 patients enrolled in the high-dose group. Treatment with dexamethasone 20 mg daily compared with dexamethasone 6 mg daily did not result in better clinical improvement based on OSCI on day 28 (71.2% vs. 78.2%; odds ratio, 1.45 [0.55–3.86]; p = 0.403). For participants who required high-flow oxygen or noninvasive ventilation at randomization, the 6-mg group had better survival than the 20-mg group on day 28 (100% vs. 57.1%; p = 0.025). Although more participants in the 6-mg group received immune modulators (40% vs. 21.2%; p = 0.035), 50% of death cases in the 20-mg group who required high-flow oxygen or noninvasive ventilation at randomization received immune modulators.

Conclusions

Dexamethasone 20 mg daily did not result in better clinical outcome improvement, and was probably associated with higher 28-day mortality in patients on high-flow oxygen or noninvasive ventilation, compared with dexamethasone 6 mg daily.

Trial registration

Clinialtrials.gov number, NCT04707534, registered January 13, 2021
Assessing distribution changes of selected native and alien invasive plant species under changing climatic conditions in Nyeri County, Kenya
Authors: Waititu Julius Maina , Mundia Charles Ndegwa , Sichangi Arthur W.
Published: October 2022

The role of climate change in enhancing bio-invasions in natural environments needs to be assessed to provide baseline information for effective species management and policy formulations. In this study, potential habitat suitability maps were generated through Ecological Niche Modeling for five problematic alien and native species in current and future climate simulations for the periods 2050s and 2070s under RCP2.6, RCP4.5, and RCP8.5 emission scenarios. Projected current binary suitability maps showed that 67%, 40%, 28%, 68%, and 54% of the total study area ~ 3318 Km2 is suitable for C. decapetala, L. camara, O. stricta, S. didymobotrya and S. campylacanthum species, respectively. Assuming unlimited species dispersal, two of these species, C. decapetala and S. didymobotrya, were observed to have consistent gradual increase in potential habitats and no habitat losses under the three RCPs by the end of the 2050 and 2070 future periods. The highest recorded relative potential habitat increase was observed for O. stricta at ~205% under RCP2.6 and ~223% under RCP8.5. Although L. camara and O. stricta were observed to have habitat losses, the losses will be very low as compared to that of S. campylacanthum. L. camara and O. stricta relative habitat losses were predicted to be between ~1% under RCP2.6 to ~4.5% under RCP8.5 by 2070 while that of S. campylacanthum was between ~50% under RCP2.6 to ~68% under RCP8.5 by the year 2070. From this study we conclude that the target study species are expected to remain a big threat to inhabited areas as well as biodiversity hotspot areas especially in the Mt. Kenya and the Aberdare forest and national park reserves under climate change. The information generated through this study can be used to inform policy on prioritizing management of these species and subsequent determination of their absolute distributions within the area.
Four common vitamin D receptor polymorphisms and coronary artery disease susceptibility: A trial sequential analysis
Authors: Yan Xiaofei , Wei Yuzhen , Wang Dan , Zhao Jiangtao , Zhu Kui , Liu Yuan , Tao Hailong
Published: October 2022

Background

Studies on the susceptibility of vitamin D receptor (VDR) polymorphisms to coronary artery disease (CAD) reached controversial results. We performed this study for a more accurate evaluation between the VDR polymorphisms and CAD susceptibility.

Methods

PubMed, Embase, CNKI, Wan Fang, and VIP databases were searched. The odds ratios (ORs) and 95% confidence intervals (95% CIs) were used to evaluate the associations. Trial sequential analysis (TSA) was introduced to estimate the positive associations. The potential functions of the VDR polymorphisms were analyzed based on the SNPinfo and ENSEMBL databases.

Results

Thirteen studies were finally included. In the overall analysis, increased CAD risks were observed in the VDR rs1544410 polymorphism and verified by the TSA; for the rs2228570 and rs731236 polymorphisms, significant associations with high heterogeneity were detected; decreased risk was remarkably observed for the rs7975232 polymorphism. In the subgroup analysis, wide associations with reduced heterogeneity were observed in the rs2228570, rs1544410, and rs731236 polymorphisms. The RNAfold analysis indicated the mutant G allele of the rs1544410 polymorphism was easier to disperse from the DNA double helix structure and may have a potential crucial role in the VDR transcription process.

Conclusions

Our analysis supports the role of the rs1544410 polymorphism in the VDR gene as a risk factor for CAD. The VDR rs2228570 and rs731236 polymorphisms were associated with increased CAD risks in the White population. Restrict decreased CAD risk was firstly discovered in the rs7975232 polymorphism.

Limitations

Firstly, the language was restricted to English and Chinese, which will cause the limited number of studies included; secondly, other unknown polymorphisms in VDR polymorphisms could also be associated the CAD susceptibility, and more case-control studies with comprehensive clinical outcomes and GWAS studies were required; thirdly, the rs1544410, rs7975232 and rs731236 polymorphism are in strong LD, haploid factors with CAD risk need to be considered; fourthly, the mechanisms of the VDR polymorphism on the VDR gene or RNA or protein were not discussed enough, further mechanistic studies are required; at last, genetic factor was the one side for CAD susceptibility, the interaction between environmental risk factors should be considered.
Cryo-electron structures of the extreme thermostable enzymes Sulfur Oxygenase Reductase and Lumazine Synthase
Authors: Sobhy Mohamed A. , Zhao Lingyun , Anjum Dalaver , Behzad Ali , Takahashi Masateru , Tehseen Muhammad , Biasio Alfredo De , Sougrat Rachid , Hamdan Samir
Published: October 2022

Thermostable enzymes have the potential for use in a wide variety of biotechnological applications. Cryo-electron microscopy (cryo-EM) enables the imaging of biomolecules in their native aqueous environment. Here, we present high resolution cryo-EM structures of two thermostable enzymes that exhibit multimeric cage-like structures arranged into two different point-group symmetries. First, we determined the structure of the Sulfur Oxygenase Reductase (SOR) enzyme that catalyzes both the oxygenation and disproportionation of elemental sulfur in Archea and is composed of 24 homomeric units each of MW ≃ 35 kDa arranged in octahedral symmetry. The structure of SOR fromAcidianus ambivalens(7X9W) was determined at 2.78 Å resolution. The active site of each subunit inside the central nanocompartment is composed of Fe3+coordinated to two water molecules and the three amino acids (H86, H90 and E114). Second, we determined the structure of Lumazine Synthase (LS) fromAquifex aeolicus(7X7M) at 2.33 Å resolution. LS forms a cage-like structure consisting of 60 identical subunits each of MW ≃ 15 kDa arranged in a strict icosahedral symmetry. The LS subunits are interconnected by ion-pair network. Due to their thermostability and relatively easy purification scheme, both SOR and LS can serve as a model for the catalytic and structural characterization of biocatalysts as well as a benchmark for cryo-EM sample preparation, optimization of the acquisition parameters and 3D reconstruction.
Open banking: A bibliometric analysis-driven definition
Authors: Briones de Araluze Gorka Koldobika , Cassinello Plaza Natalia
Published: October 2022

“Open banking,” as a concept, was initially developed by a UK regulation to foster competition in banking through sharing client data (with their consent) amongst competitors. Today, it is regulated in several most relevant banking jurisdictions. Despite its growing relevance, consensus about the definition of open banking is lacking. This study examines 282 articles on open banking using bibliometric clustering techniques. Moreover, within the 282 articles and applying discourse analysis, we analyze 47 idiosyncratic definitions of open banking to test an integral framework that supports our proposed definition of the concept. Our study contributes to the literature by providing a generalized multidisciplinary definition of open banking. It identifies four main drivers behind the concept: business model change, client data sharing, incorporation of technological companies (fintechs and others), and regulation. These four elements, which should be considered in new regulations in the globalized banking sector, foresee open banking as a critical enabler of a new strategic dynamic in banking.
Type 1 and Type 2 Epstein-Barr viruses induce proliferation, and inhibit differentiation, in infected telomerase-immortalized normal oral keratinocytes
Authors: Singh Deo R. , Nelson Scott E. , Pawelski Abigail S. , Cantres-Velez Juan A. , Kansra Alisha S. , Pauly Nicholas P. , Bristol Jillian A. , Hayes Mitchell , Ohashi Makoto , Casco Alejandro , Lee Denis , Fogarty Stuart A. , Lambert Paul F. , Johannsen Eric C. , Kenney Shannon C.
Published: October 2022

Differentiated epithelial cells are an important source of infectious EBV virions in human saliva, and latent Epstein-Barr virus (EBV) infection is strongly associated with the epithelial cell tumor, nasopharyngeal carcinoma (NPC). However, it has been difficult to model how EBV contributes to NPC, since EBV has not been shown to enhance proliferation of epithelial cells in monolayer culture in vitro and is not stably maintained in epithelial cells without antibiotic selection. In addition, although there are two major types of EBV (type 1 (T1) and type 2 (T2)), it is currently unknown whether T1 and T2 EBV behave differently in epithelial cells. Here we inserted a G418 resistance gene into the T2 EBV strain, AG876, allowing us to compare the phenotypes of T1 Akata virus versus T2 AG876 virus in a telomerase-immortalized normal oral keratinocyte cell line (NOKs) using a variety of different methods, including RNA-seq analysis, proliferation assays, immunoblot analyses, and air-liquid interface culture. We show that both T1 Akata virus infection and T2 AG876 virus infection of NOKs induce cellular proliferation, and inhibit spontaneous differentiation, in comparison to the uninfected cells when cells are grown without supplemental growth factors in monolayer culture. T1 EBV and T2 EBV also have a similar ability to induce epithelial-to-mesenchymal (EMT) transition and activate canonical and non-canonical NF-κB signaling in infected NOKs. In contrast to our recent results in EBV-infected lymphoblastoid cells (in which T2 EBV infection is much more lytic than T1 EBV infection), we find that NOKs infected with T1 and T2 EBV respond similarly to lytic inducing agents such as TPA treatment or differentiation. These results suggest that T1 and T2 EBV have similar phenotypes in infected epithelial cells, with both EBV types enhancing cellular proliferation and inhibiting differentiation when growth factors are limiting.