bluuebunny/crossref_metadata_2025 huggingface dataset:
Immune Responses Are Differentially Regulated by Root, Stem, Leaf, and Flower Extracts of Female and Male CBD Hemp (Cannabis sativa L.) Plants
Authors: Esposito Laura G. A. , Overbaugh Ezekial , Xiong Jia , Rathinasabapathy Thirumurugan , Komarnytsky Slavko , da Silva Derly José Henriques , Esposito Debora A.
Published: October 2021

Industrial hemp (Cannabis sativa L.) has many applications, including the production of textiles, agricultural extracts, nutritional products, and botanicals enriched with cannabinoids and full-spectrum terpenes naturally present in the plant. In this study, the dynamics of distribution and accumulation of 10 main cannabinoids in hemp were quantified. Hemp bioactive compounds were evaluated for anti-inflammatory activity in lipopolysaccharide-induced RAW 264.7 macrophage cells. While all tissues of hemp showed moderate anti-inflammatory properties, female flowers demonstrated the highest activity. CBD showed the strongest anti-inflammatory activity with suppression of nitric oxide production at 2 μg/mL and the reduced expressions of the pro-inflammatory genes COX-2, IL-6, and TNF-α at as low as 2 ng/mL. The topical hemp inflorescences (1–50 μg/mL) and CBD alone (20–200 ng/mL) also improved mitochondrial respiration. These data contribute to the future development of agricultural and plant management techniques to produce hemp with specific metabolite profiles to selectively support immune health.
Accurate Absorption Energy Calculations in Solution Using the Reference Interaction Site Model Self-Consistent Field Including the Constrained Spatial Electron Density Distribution
Authors: Yokogawa Daisuke
Published: October 2021

The solvation effect is an important factor determining the properties of molecules in solution. The reference interaction site model (RISM) is a powerful method to treat the solvation effect with pair-correlation functions, such as a radial distribution function. This study developed a hybrid method between quantum mechanics and RISM using the spatial electron density distributions on each atomic site (RISM-SCF-cSED). Sophisticated quantum mechanical approaches can be used to consider the solvation effect because the computational cost of RISM-SCF-cSED is reasonable. In this study, the absorption energies of 5-(dimethylamino)-2,4-pentadienal in various solutions were calculated using RISM-SCF-cSED. The experimental data were well reproduced with an average errors of ∼0.06 eV, using multi-reference perturbation theory.
Non-Invasive Continuous Measurement of Haemodynamic Parameters—Clinical Utility
Authors: Bodys-Pełka Aleksandra , Kusztal Maciej , Boszko Maria , Główczyńska Renata , Grabowski Marcin
Published: October 2021

The evaluation and monitoring of patients’ haemodynamic parameters are essential in everyday clinical practice. The application of continuous, non-invasive measurement methods is a relatively recent solution. CNAP, ClearSight and many other technologies have been introduced to the market. The use of these techniques for assessing patient eligibility before cardiac procedures, as well as for intraoperative monitoring is currently being widely investigated. Their numerous advantages, including the simplicity of application, time- and cost-effectiveness, and the limited risk of infection, could enforce their further development and potential utility. However, some limitations and contradictions should also be discussed. The aim of this paper is to briefly describe the new findings, give practical examples of the clinical utility of these methods, compare them with invasive techniques, and review the literature on this subject.
COVID-19 Advanced Care
Authors: Fouka Evangelia , Kalomenidis Ioannis , Gianniou Niki , Gida Sofia , Steiropoulos Paschalis
Published: October 2021

The coronavirus disease 2019 (COVID-19) pandemic, related to the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused a worldwide sudden and substantial burden in public health due to an enormous increase in hospitalizations for pneumonia with the multiorgan disease. Treatment for individuals with COVID-19 includes best practices for supportive management of acute hypoxic respiratory failure. Emerging data indicate that dexamethasone therapy reduces 28-day mortality in patients requiring supplemental oxygen compared with usual care, and ongoing trials are testing the efficacy of antiviral therapies, immune modulators and anticoagulants in the prevention of disease progression and complications, while monoclonal antibodies and hyperimmune globulin may provide additional preventive strategies. Consensus suggestions can standardize care, thereby improving outcomes and facilitating future research. This review discusses current evidence regarding the pharmacotherapy of COVID-19.
The Effect of TNF-α Inhibitors on Nail Psoriasis and Psoriatic Arthritis—Real-World Data from Dermatology Practice
Authors: Kokolakis Georgios , Sabat Robert , Fischer Imma , Gomis-Kleindienst Susana , Fritz Björn , Burmester Gerd-Rüdiger , Ghoreschi Kamran , Ohrndorf Sarah
Published: October 2021

Patients with psoriatic arthritis (PsA) often develop joint symptoms years after their initial diagnosis of psoriasis disease; therefore, dermatologists should test for and detect PsA early. In this study, we focused on patients with psoriasis with both nail and joint disease being treated with tumor necrosis factor-α inhibitors by dermatologists. We performed a noninterventional, prospective, multicenter, and open-label study to evaluate the effectiveness of adalimumab, etanercept, or infliximab over 24 months of continuous therapy in patients with moderate to severe plaque-type psoriasis (Pso) and PsA. Disease assessments with the Psoriasis Area and Severity Index, Nail Psoriasis Severity Index (NAPSI), joint assessment, Dermatology Life Quality Index (DLQI), and Health Assessment Questionnaire (HAQ) instruments were performed every 3 months for the first year and twice annually thereafter. The cohort included 100 patients with Pso, nail psoriasis, and PsA. A significant reduction of NAPSI was observed 3 months after therapy initiation compared with the baseline (mean ± SD, 22.9 ± 17.8 vs. 33.8 ± 21.4; p < 0.001). Similarly, the mean ± SD number of both tender and swollen joints decreased significantly within the first 3 months of treatment, from 10.8 ± 11.5 to 6.4 ± 10.3 (p < 0.001) and from 6.4 ± 9.5 to 3.1 ± 7.2 (p < 0.001), respectively. Additionally, the distal interphalangeal joint involvement improved throughout the observation time, and DLQI and HAQ scores decreased. Improvements in control of skin, nail, and joint symptoms were seen, as well as in patients’ quality of life and functionality. Dermatologists have an important role not only in PsA diagnosis but also in PsA long-term care.
RETRACTED: Salhi (2021). The Relationship between CEO Psychological Biases, Corporate Governance and Corporate Social Responsibility. Journal of Risk and Financial Management 14: 317
Authors: Salhi Bassem
Published: October 2021

The journal retracts the article “The Relationship between CEO Psychological Biases, Corporate Governance and Corporate Social Responsibility” (Salhi 2021), cited above [...]
Impact of Urbanization and Industrial Structure on Carbon Emissions: Evidence from Huaihe River Eco-Economic Zone
Authors: Pang Qinghua , Zhou Weimo , Zhao Tianxin , Zhang Lina
Published: October 2021

Rapid urbanization in China greatly contributes to carbon emissions, while the industrial structure greatly contributes to changes in the variation of carbon emissions. This research addresses the impact of urbanization and industrial structure on carbon emissions from 2010 to 2018, by focusing on the Huaihe River Eco-economic Zone, which is an important economic corridor along the north–south division of China. Although many studies have focused on investigating the impact of urbanization or industrial structure on carbon emissions, few studies further addressed an analysis of the impact of both on carbon emissions, using multiple measurement models. This paper reveals the holistic and local impact of industrial structure and urbanization on carbon emissions, by integrating a threshold regression model with geographically weighted regression. The results are as follows: (1) From a holistic point of view, industrial structure and urbanization had both, single threshold, and double threshold effects on carbon emissions in the Huaihe River Eco-economic Zone. (2) From a regional perspective, the coefficients of industrial structure on carbon emissions were all positive, but the rate of increase gradually slowed down. The coefficients of urbanization on carbon emissions were all negative, reaching a maximum value of negative effect in 2013. Understanding the holistic and local impact of urbanization and industrial structure on carbon emissions provides governments with differentiated and forward-looking suggestions for mitigating carbon emissions in the Huaihe River Eco-economic Zone.
The Perceived Value and Future Behavioral Intentions in Ecotourism: A Study in the Mediterranean Natural Parks from Spain
Authors: Carvache-Franco Mauricio , Carrascosa-López Conrado , Carvache-Franco Wilmer
Published: October 2021

Ecotourism is becoming increasingly important in natural parks because it raises the value of the environment for the visitor. The present study aimed to (i) establish the factors of the perceived value in ecotourism and (ii) analyze the predictive relationships of the dimensions of the perceived value with the satisfaction and loyalty of ecotourists. The study was carried out in the Albufera Natural Park and the Serranía de Cuenca Natural Park, two protected areas in the Mediterranean area of Spain. The sample collected in situ consisted of 349 valid questionnaires. An exploratory factor analysis (EFA), a confirmatory factor analysis (CFA), and multiple regression techniques were performed for data analysis. The results established three dimensions in the perceived value: functional and emotional, economic, and social, being the “functional and emotional” value the most significant predictor of satisfaction and loyalty of ecotourists. The findings will allow institutions to have a management guide for protected areas.
Between Loss and Salvage: Kabyles and Syrian Christians Negotiate Heritage, Linguistic Authenticity and Identity in Europe
Authors: Armbruster Heidi , Belabbas Souhila
Published: October 2021

This paper brings together two different communities, Kabyles (Amazighs) and Syrian Christians, who are nevertheless marked by some commonalities: a strong diasporic dispersal as a historical experience, political, cultural and linguistic marginalization in their countries of origin, the deep association of collective identity with an “endangered” heritage language, a lived experience of multilingualism, and a post-emigration struggle of language maintenance and transmission. The Kabyles have roots in northern Algeria, and associate their language, Kabyle, with a pre-Arabized history of northern Africa, with claims to cultural authenticity and indigeneity. This paper focuses on research conducted in the UK, a relatively new immigrant setting for this community. The Syrian Christians originate from Turkey and have dispersed across different European countries since the 1960s. They make strong identity claims to Aramaic, “the language of Jesus”, yet have also found its preservation and intergenerational transmission challenging. This paper focuses on research conducted in the German speaking context. Drawing on ethnographic research with these communities, we bring their post-migration language preservation activisms into a dialogue. This shows the enduring significance of the heritage language for social, cultural and historical identity, despite considerable language decline. It also demonstrates that the current survival of the “mother tongue” hinges on multilingual and multi-sited language activisms which bear the hallmarks of both new creativities and diminishing fluencies.
Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells
Authors: Chen Chun , Yang Jai-Sing , Lu Chi-Cheng , Wu Yu-Tse , Chen Fu-An
Published: October 2021

Nonsteroidal anti-inflammatory drugs (NSAIDs) are used to treat inflammation and pain and even to prevent the progression of cardiovascular disease. They have become widely used because of their effectiveness, especially among athletes performing high-intensity training. Indomethacin is used for pain management in sports medicine and is highly effective and versatile. However, several clinical studies have reported that indomethacin induces acute renal damage. In the present study, we determined that indomethacin reduced human embryonic kidney 293 (HEK293) cell viability in a concentration-dependent manner by triggering apoptosis. In addition, we demonstrated the effect of quercetin on indomethacin-treated HEK293 cells by inactivating the caspase-3 and caspase-9 signals. Furthermore, quercetin reduced ROS production and increased mitochondrial membrane potential (ΔΨm) in indomethacin-treated HEK293 cells. Our results indicate that quercetin can interrupt the activated caspase and mitochondrial pathway induced by indomethacin in HEK293 cells and affect apoptotic mRNA expression. Quercetin can protect against indomethacin-induced HEK293 cell apoptosis by regulating abnormal ΔΨm and apoptotic mRNA expression.
A Hybrid Approach of ANFIS—Artificial Bee Colony Algorithm for Intelligent Modeling and Optimization of Plasma Arc Cutting on Monel™ 400 Alloy
Authors: Siva Kumar Mahalingam , Rajamani Devaraj , Abouel Nasr Emad , Balasubramanian Esakki , Mohamed Hussein , Astarita Antonello
Published: October 2021

This paper focusses on a hybrid approach based on genetic algorithm (GA) and an adaptive neuro fuzzy inference system (ANFIS) for modeling the correlation between plasma arc cutting (PAC) parameters and the response characteristics of machined Monel 400 alloy sheets. PAC experiments are performed based on box-behnken design methodology by considering cutting speed, gas pressure, arc current, and stand-off distance as input parameters, and surface roughness (Ra), kerf width (kw), and micro hardness (mh) as response characteristics. GA is efficaciously utilized as the training algorithm to optimize the ANFIS parameters. The training, testing errors, and statistical validation parameter results indicated that the ANFIS learned by GA outperforms in the forecasting of PAC responses compared with the results of multiple linear regression models. Besides that, to obtain the optimal combination PAC parameters, multi-response optimization was performed using a trained ANFIS network coupled with an artificial bee colony algorithm (ABC). The superlative responses, such as Ra of 1.5387 µm, kw of 1.2034 mm, and mh of 176.08, are used to forecast the optimum cutting conditions, such as a cutting speed of 2330.39 mm/min, gas pressure of 3.84 bar, arc current of 45 A, and stand-off distance of 2.01 mm, respectively. Furthermore, the ABC predicted results are validated by conducting confirmatory experiments, and it was found that the error between the predicted and the actual results are lower than 6.38%, indicating the adoptability of the proposed ABC in optimizing real-world complex machining processes.
Advanced Evaluation of the Freeze–Thaw Damage of Concrete Based on the Fracture Tests
Authors: Kucharczyková Barbara , Šimonová Hana , Kocáb Dalibor , Topolář Libor
Published: October 2021

This paper presents the results of an experimental program aimed at the assessment of the freeze–thaw (F–T) resistance of concrete based on the evaluation of fracture tests accompanied by acoustic emission measurements. Two concretes of similar mechanical characteristics were manufactured for the experiment. The main difference between the C1 and C2 concrete was in the total number of air voids and in the A300 parameter, where both parameters were higher for C1 by about 35% and 52%, respectively. The evaluation of the fracture characteristics was performed on the basis of experimentally recorded load–deflection and load–crack mouth opening displacement diagrams using two different approaches: linear fracture mechanics completed with the effective crack model and the double-K model. The results show that both approaches gave similar results, especially if the nonlinear behavior before the peak load was considered. According to the results, it can be stated that continuous AE measurement is beneficial for the assessment of the extent of concrete deterioration, and it suitably supplements the fracture test evaluation. A comparison of the results of fracture tests with the resonance method and splitting tensile strength test shows that all testing methods led to the same conclusion, i.e., the C1 concrete was more F–T-resistant than C2. However, the fracture test evaluation provided more detailed information about the internal structure deterioration due to the F–T exposure.
Combining Fiber Enzymatic Pretreatments and Coupling Agents to Improve Physical and Mechanical Properties of Hemp Hurd/Wood/Polypropylene Composite
Authors: Li Xiaoping , Qiang Mingli , Yang Mingwei , Morrell Jeffrey J. , Zhang Neng
Published: October 2021

Natural fiber/plastic composites combine the low density and excellent mechanical properties of the natural fiber with the flexibility and moisture resistance of the plastic to create materials tailored to specific applications in theory. Wood/plastic composites (WPC) are the most common products, but many other fibers are being explored for this purpose. Among the more common is hemp hurd. Natural fibers are hydrophilic materials and plastics are hydrophobic, therefore one problem with all of these products is the limited ability of the fiber to interact with the plastic to create a true composite. Thus, compatibilizers are often added to enhance interactions, but fiber pretreatments may also help improve compatibility. The effects of pectinase or cellulase pretreatment of wood/hemp fiber mixtures in combination with coupling agents were evaluated in polypropylene panels. Pretreatments with pectinase or cellulase were associated with reduced thickness swell (TS24h) as well as increased modulus of rupture and modulus of elasticity. Incorporation of 5.0% silane or 2.5% silane/2.5% titanate as a coupling agent further improved pectinase-treated panel properties, but was associated with diminished properties in cellulase treated fibers. Combinations of enzymatic pretreatment and coupling agents enhanced fiber/plastic interactions and improved flexural properties, but the effects varied with the enzyme or coupling agent employed. The results illustrate the potential for enhancing fiber/plastic interactions to produce improved composites.
Declining Performance of Silicone-Based Magnetorheological Elastomers after Accelerated Weathering
Authors: Wibowo Wibowo , Lenggana Bhre Wangsa , Ubaidillah Ubaidillah , Ariawan Dody , Imaduddin Fitrian , Mazlan Saiful Amri , Choi Seung-Bok
Published: October 2021

Magnetorheological elastomers (MRE)-based products are usually located in an area directly exposed to sunlight and rain. However, there is no specific research on the behavior of MRE after accelerated weathering. Therefore, in this study, the changes to the chemical and rheological properties of both isotropic and anisotropic MRE after accelerated weathering were examined. Treated and untreated specimens were compared. MRE specimens with 40% by weight CIP were prepared with no current excitation and another sample was prepared with 1.5 T of magnetic flux density. Each specimen was treated in an accelerated weathering machine, Q-Sun Xe-1 Xenon Test Chamber, under a UV light exposure cycle and water spray. A material characterization was carried out using FTIR and a rheometer to determine the changes to the chemical and rheological properties. The morphological analysis results showed that after the weather treatment, the surface was rough and more cavities occurred. The rheometer test results showed a significant decrease in the storage modulus of each treated MRE specimen, unlike the untreated MRE specimens. The decrease in the storage modulus value with currents of 0, 1, 2, and 3 Amperes was 66.67%, 78.9%, 85.2%, and 80.5%, respectively. Meanwhile, FTIR testing showed a change in the wave peak between the untreated and treated MRE specimens. Thermogravimetric analysis (TGA) also showed a decrease in MRE weight for each specimen. However, for both treated and untreated MRE specimens, the decrease in TGA was not significantly different. In all the tests carried out on the MRE samples, weather acceleration treatment caused significant changes. This is an important consideration for developers who choose silicone as the MRE matrix.
Influence of Material Parameters on the Contact Pressure Characteristics of a Multi-Disc Clutch
Authors: Liu Yujian , Chen Man , Yu Liang , Wang Liang , Feng Yuqing
Published: October 2021

As an essential part of the transmission, the life of the clutch directly affects the stability of the transmission. In this paper, a finite element model and a thermodynamic numerical model of a multi-disc clutch are established to investigate the influence of material parameters on the contact pressure distribution. The pressure distribution index (PDI) is firstly proposed to evaluate the pressure difference among friction pairs. Moreover, the correctness of the numerical model is verified by the clutch static pressure experiment. The results show that increasing the elastic modulus and Poisson’s ratio of the backplate can effectively improve the uniformity of the contact pressure. However, the variations in material parameters of other clutch components can not easily smooth the pressure difference. Therefore, optimized material parameters for the clutch are proposed, where the maximum pressure and temperature differences are reduced by about 27.2% and 10.3%, respectively.
Effect of Bonding Strength on Electromigration Failure in Cu–Cu Bumps
Authors: Shie Kai-Cheng , Hsu Po-Ning , Li Yu-Jin , Tu K. N. , Chen Chih
Published: October 2021

In microelectronic packaging technology for three-dimensional integrated circuits (3D ICs), Cu-to-Cu direct bonding appears to be the solution to solve the problems of Joule heating and electromigration (EM) in solder microbumps under 10 μm in diameter. However, EM will occur in Cu–Cu bumps when the current density is over 106 A/cm2. The surface, grain boundary, and the interface between the Cu and TiW adhesion layer are the three major diffusion paths in EM tests, and which one may lead to early failure is of interest. This study showed that bonding strength affects the outcome. First, if the bonding strength is not strong enough to sustain the thermal mismatch of materials during EM tests, the bonding interface will fracture and lead to an open circuit of early failure. Second, if the bonding strength can sustain the bonding structure, voids will form at the passivation contact area between the Cu–Cu bump and redistribution layer (RDL) due to current crowding. When the void grows along the passivation interface and separates the Cu–Cu bump and RDL, an open circuit can occur, especially when the current density and temperature are severe. Third, under excellent bonding, when the voids at the contact area between the Cu–Cu bump and RDL do not merge together, the EM lifetime can be more than 5000 h.
Trochoidal Milling Path with Variable Feed. Application to the Machining of a Ti-6Al-4V Part
Authors: García-Hernández César , Garde-Barace Juan-José , Valdivia-Sánchez Juan-Jesús , Ubieto-Artur Pedro , Bueno-Pérez José-Antonio , Cano-Álvarez Basilio , Alcázar-Sánchez Miguel-Ángel , Valdivia-Calvo Francisco , Ponz-Cuenca Rubén , Huertas-Talón José-Luis , Kyratsis Panagiotis
Published: October 2021

Trochoidal milling is a well-stablished machining strategy which still allows for the introduction of new approaches. This strategy can be applied to any kind of material, although it is usually associated to advanced materials, such as titanium and nickel alloys. This study is based on the adaptation of the feed speed of a milling tool with Ti-6Al-4V, so the chip width can be maintained constant without modifying the path geometry. A singularity in the experimental stage was to mill an Archimedes spiral groove instead of the conventional straight grooves. This made it possible to obtain a concave wall as well as a convex one and to optimize the amount of material used. The time efficiency compared to a constant feed, was slightly superior to 20%, reducing tool wear also. These techniques require milling machines with high mechanical and kinematic performance, as well as the absence of clearance between joints and a high acceleration capacity.
Causality Distance Measures for Multivariate Time Series with Applications
Authors: Anastasiou Achilleas , Hatzopoulos Peter , Karagrigoriou Alex , Mavridoglou George
Published: October 2021

In this work, we focus on the development of new distance measure algorithms, namely, the Causality Within Groups (CAWG), the Generalized Causality Within Groups (GCAWG) and the Causality Between Groups (CABG), all of which are based on the well-known Granger causality. The proposed distances together with the associated algorithms are suitable for multivariate statistical data analysis including unsupervised classification (clustering) purposes for the analysis of multivariate time series data with emphasis on financial and economic data where causal relationships are frequently present. For exploring the appropriateness of the proposed methodology, we implement, for illustrative purposes, the proposed algorithms to hierarchical clustering for the classification of 19 EU countries based on seven variables related to health resources in healthcare systems.
Oxygen: A Stimulus, Not “Only” a Drug
Authors: Balestra Costantino , Kot Jacek
Published: October 2021

Depending on the oxygen partial pressure in a tissue, the therapeutic effect of oxygenation can vary from simple substance substitution up to hyperbaric oxygenation when breathing hyperbaric oxygen at 2.5–3.0 ATA. Surprisingly, new data showed that it is not only the oxygen supply that matters as even a minimal increase in the partial pressure of oxygen is efficient in triggering cellular reactions by eliciting the production of hypoxia-inducible factors and heat-shock proteins. Moreover, it was shown that extreme environments could also interact with the genome; in fact, epigenetics appears to play a major role in extreme environments and exercise, especially when changes in oxygen partial pressure are involved. Hyperbaric oxygen therapy is, essentially, “intermittent oxygen” exposure. We must investigate hyperbaric oxygen with a new paradigm of treating oxygen as a potent stimulus of the molecular network of reactions.
On the Role of Electrostatic Repulsion in Topological Defect-Driven Membrane Fission
Authors: Gongadze Ekaterina , Mesarec Luka , Kralj Samo , Kralj-Iglič Veronika , Iglič Aleš
Published: October 2021

Within a modified Langevin Poisson–Boltzmann model of electric double layers, we derived an analytical expression for osmotic pressure between two charged surfaces. The orientational ordering of the water dipoles as well as the space dependencies of electric potentials, electric fields, and osmotic pressure between two charged spheres were taken into account in the model. Thus, we were able to capture the interaction between the parent cell and connected daughter vesicle or the interactions between neighbouring beads in necklace-like membrane protrusions. The predicted repulsion between them can facilitate the topological antidefect-driven fission of membrane daughter vesicles and the fission of beads of undulated membrane protrusions.
Developing a Thin Film Composite Membrane with Hydrophilic Sulfonated Substrate on Nonwoven Backing Fabric Support for Forward Osmosis
Authors: Sahebi Soleyman , Kahrizi Mohammad , Fadaie Nasim , Hadadpour Soheil , Ramavandi Bahman , Gonzales Ralph Rolly
Published: October 2021

This study describes the fabrication of sulfonated polyethersulfone (SPES) as a super-hydrophilic substrate for developing a composite forward osmosis (FO) membrane on a nonwoven backing fabric support. SPES was prepared through an indirect sulfonation procedure and then blended with PES at a certain ratio. Applying SPES as the substrate affected membrane properties, such as porosity, total thickness, morphology, and hydrophilicity. The PES-based FO membrane with a finger-like structure had lower performance in comparison with the SPES based FO membrane having a sponge-like structure. The finger-like morphology changed to a sponge-like morphology with the increase in the SPES concentration. The FO membrane based on a more hydrophilic substrate via sulfonation had a sponge morphology and showed better water flux results. Water flux of 26.1 L m−2 h−1 and specific reverse solute flux of 0.66 g L−1 were attained at a SPES blend ratio of 50 wt % when 3 M NaCl was used as the draw solution and DI water as feed solution under the FO mode. This work offers significant insights into understanding the factors affecting FO membrane performance, such as porosity and functionality.
Effect of Quenching and Partitioning Heat Treatment on the Fatigue Behavior of 42SiCr Steel
Authors: Thomä Marco , Wagner Guntram
Published: October 2021

The manufacturing of advanced high-strength steels with enhanced ductility is a persistent aim of research. The ability of a material to absorb high loads while showing a high deformation behavior is a major task for many industrial fields like the mobility sector. Therefore, the material properties of advanced high-strength steels are one of the most important impact factors on the resulting cyclic fatigue behavior. To adjust advanced material properties, resulting in high tensile strengths as well as an enhanced ductility, the heat treatment process of quenching and partitioning (QP) was developed. The quenching takes place in a field between martensite start and martensite finish temperature and the subsequent partitioning is executed at slightly elevated temperatures. Regarding the sparsely investigated field of fatigue research on quenched and partitioned steels, the present work investigates the influence of a QP heat treatment on the resulting microstructure by light and scanning electron microscopy as well as on the mechanical properties such as tensile strength and resistance against fatigue regarding two different heat treatment conditions (QP1, QP2) in comparison to the cold-rolled base material of 42SiCr steel. Therefore, the microscopic analysis proved the presence of a characteristic quenched and partitioned microstructure consisting of a martensitic matrix and partial areas of retained austenite, whereas carbides were also present. Differences in the amount of retained austenite could be observed by using X-ray diffraction (XRD) for the different QP routes, which influence the mechanical properties resulting in higher tensile strength of about 2000 MPa for QP1 compared to about 1600 MPa for QP2. Furthermore, the transition for the fatigue limit was approximated by using stepwise load increase tests (LIT) and afterwards verified by constant amplitude tests (CAT) in accordance with the staircase method, whereas the QP1 condition reached the highest fatigue strength of 900 MPa. Subsequent light and scanning electron microscopy of selected fractured surfaces and runouts showed a different behavior regarding the size of the fatigue fracture area and also differences in the microstructure of these runouts.
Ultrasensitive Detection of SARS-CoV-2 Spike Proteins Using the Thio-NAD Cycling Reaction: A Preliminary Study before Clinical Trials
Authors: Kyosei Yuta , Namba Mayuri , Makioka Daiki , Kokubun Ayumi , Watabe Satoshi , Yoshimura Teruki , Sasaki Tadahiro , Shioda Tatsuo , Ito Etsuro
Published: October 2021

To help control the global pandemic of coronavirus disease 2019 (COVID-19), we developed a diagnostic method targeting the spike protein of the virus that causes the infection, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We applied an ultrasensitive method by combining a sandwich enzyme-linked immunosorbent assay (ELISA) and the thio-nicotinamide adenine dinucleotide (thio-NAD) cycling reaction to quantify spike S1 proteins. The limit of detection (LOD) was 2.62 × 10−19 moles/assay for recombinant S1 proteins and 2.6 × 106 RNA copies/assay for ultraviolet B-inactivated viruses. We have already shown that the ultrasensitive ELISA for nucleocapsid proteins can detect ultraviolet B-inactivated viruses at the 104 RNA copies/assay level, whereas the nucleocapsid proteins of SARS-CoV-2 are difficult to distinguish from those in conventional coronaviruses and SARS-CoV. Thus, an antigen test for only the nucleocapsid proteins is insufficient for virus specificity. Therefore, the use of a combination of tests against both spike and nucleocapsid proteins is recommended to increase both the detection sensitivity and testing accuracy of the COVID-19 antigen test. Taken together, our present study, in which we incorporate S1 detection by combining the ultrasensitive ELISA for nucleocapsid proteins, offers an ultrasensitive, antigen-specific test for COVID-19.
Pilot Study on the Forehead Skin Microbiome and Short Chain Fatty Acids Depending on the SC Functional Index in Korean Cohorts
Authors: Park Haryung , Arellano Karina , Lee Yuri , Yeo Subin , Ji Yosep , Ko Joontae , Holzapfel Wilhelm
Published: October 2021

Dry skin is one of the indicators of a compromised skin barrier. An intact skin barrier is not only important to reserve the hydration within the epidermal tissue but also to protect our skin from environmental stressors and inhibit pathogen invasion; damage to the skin barrier may lead to inflammatory skin diseases. Some microbial metabolites such as short chain fatty acids may inhibit or destroy harmful bacteria and regulate the host immune system. The impact of the skin microbiome and short chain fatty acids on skin barrier function was studied in two groups of 75 participants each. The cohort was equally divided in dry and moist skin types, based on stratum corneum (SC) functionality index (SCFI), reflecting the ratio of transepidermal water loss (TEWL). A dry group represents a low SCFI and a moist group a high SCFI. Compared with the dry skin group, propionate and Cutibacterium levels (previously known as Propionibacterium acnes) were significantly higher (p < 0.001) in the moist group. Levels of Cutibacterium were negatively correlated with those of Staphylococcus (p < 0.0001) in both dry and moist groups. The moist group also had a significantly higher propionate concentration (p < 0.001). This study showed that the microbial community and short chain fatty acid concentration may be considered as significant determinants of the SCFI of the skin.
Understanding the Effect of Stepwise Irrigation on Liquid Holdup and Hysteresis Behavior of Unsaturated Ore Heap
Authors: Wang Leiming , Yin Shenghua , Deng Bona
Published: October 2021

Liquid is a crucial medium to contain soluble oxygen, valuable metal ions, and bacteria in unsaturated heap leaching. Liquid retention behavior is the first critical issue to be considered to efficiently extract low-grade minerals or wastes. In this study, the residual liquid holdup of an unsaturated packed bed was quantitatively discussed by liquid holdup (θ), residual liquid holdup (θresidual), relative liquid holdup (θ′), and relative porosity (n*) using the designed measuring device. The detailed liquid holdup and the hysteresis behavior under stepwise irrigation are indicated and discussed herein. The results show that relative porosity of the packed bed was negatively related to particle size, and intra-particle porosity was more developed in the −4.0 + 2.0 mm packed bed. The higher liquid retention of the unsaturated packed bed could be obtained by using stepwise irrigation (incrementally improved from 0.001 to 0.1 mm/s) instead of uniform irrigation (0.1 mm/s). It could be explained in that some of the immobile liquid could not flow out of the unsaturated packed bed, and this historical irrigation could have accelerated formation of flow paths. The θ was sensitive to superficial flow rate (or irrigation rate) in that it obviously increased if a higher superficial flow rate (u) was introduced, however, the θresidual was commonly affected by n* and θ′. Moreover, the liquid hysteresis easily performed under stepwise irrigation condition, where θ and θresidual were larger at u of the decreasing flow rate stage (DFRS) instead of u of the increasing flow rate stage (IFRS). These findings effectively quantify the liquid retention and the hysteresis behavior of ore heap, and the stepwise irrigation provides potential possibility to adjust liquid retention conditions.
Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales
Authors: Gonçalves Sandra , Mansinhos Inês , Rodríguez-Solana Raquel , Pereira-Caro Gema , Moreno-Rojas José Manuel , Romano Anabela
Published: October 2021

Nanoparticles (NPs) recently emerged as new chemical elicitors acting as signaling agents affecting several processes in plant metabolism. The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe3O4) NPs (<100 nm) at different concentrations (1, 5 and 10 mg/L) to the culture media on several morphological, physiological and -biochemical parameters of in vitro shoot cultures of Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales (Lamiaceae), as well as on phenolic profile and bioactivity (antioxidant and enzyme inhibition capacities). Although some decreases in shoot number and length were observed in response to NPs, biomass production was not affected or was improved in both species. Most NPs treatments decreased total chlorophyll and carotenoid contents and increased malondialdehyde levels, an indicator of lipid peroxidation, in both species. HPLC-HR-MS analysis led to the identification of thirteen and twelve phenolic compounds, respectively, in L. viridis and T. lotocephalus extracts, being rosmarinic acid the major compound found in all the extracts. ZnO and Fe3O4 NPs induced an increase in total phenolic and rosmarinic acid contents in T. lotocephalus extracts. Additionally, some NPs treatments also increased antioxidant activity in extracts from this species and the opposite was observed for L. viridis. The capacity of the extracts to inhibit tyrosinase, acetylcholinesterase and butyrylcholinesterase enzymes was not considerably affected. Overall, NPs had a significant impact on different parameters of L. viridis and T. lotocephalus in vitro shoot cultures, although the results varied with the species and NPs type.
Bioactive Compounds of Opuntia spp. Acid Fruits: Micro and Nano-Emulsified Extracts and Applications in Nutraceutical Foods
Authors: Fernández-Luqueño Fabián , Medina-Pérez Gabriela , Pérez-Soto Elizabeth , Espino-Manzano Salvador , Peralta-Adauto Laura , Pérez-Ríos Sergio , Campos-Montiel Rafael
Published: October 2021

The acid fruit of the "xoconostle" cactus belongs to the genus Opuntia family of cacti. It is used as a functional food for its bioactive compounds. Several studies reported that xoconostle fruits have a high amount of ascorbic acid, betalains, phenols, tannins, and flavonoids. These compounds confer antioxidant, antibacterial, anti-inflammatory, and hepatoprotective gastroprotective activity. Xoconostle fruit extracts were tested by in vitro assays where the digestion conditions were simulated to measure their stability. At the same time, the extracts were protected by encapsulation (microencapsulation, multiple emulsions, and nanoemulsions). Applications of encapsulated extracts were probed in various food matrices (edible films, meat products, dairy, and fruit coatings). The xoconostle is a natural source of nutraceutical compounds, and the use of this fruit in the new food could help improve consumers’ health.
Dual Catalytic Hairpin Assembly-Based Automatic Molecule Machine for Amplified Detection of Auxin Response Factor-Targeted MicroRNA-160
Authors: Wang Lei , Dai Xing , Feng Yujian , Zhao Qiyang , Liu Lin , Xue Chang , Xiao Langtao , Wang Ruozhong
Published: October 2021

MicroRNA160 plays a crucial role in plant development by negatively regulating the auxin response factors (ARFs). In this manuscript, we design an automatic molecule machine (AMM) based on the dual catalytic hairpin assembly (D-CHA) strategy for the signal amplification detection of miRNA160. The detection system contains four hairpin-shaped DNA probes (HP1, HP2, HP3, and HP4). For HP1, the loop is designed to be complementary to miRNA160. A fragment of DNA with the same sequences as miRNA160 is separated into two pieces that are connected at the 3′ end of HP2 and 5′ end of HP3, respectively. In the presence of the target, four HPs are successively dissolved by the first catalytic hairpin assembly (CHA1), forming a four-way DNA junction (F-DJ) that enables the rearrangement of separated DNA fragments at the end of HP2 and HP3 and serving as an integrated target analogue for initiating the second CHA reaction, generating an enhanced fluorescence signal. Assay experiments demonstrate that D-CHA has a better performance compared with traditional CHA, achieving the detection limit as low as 10 pM for miRNA160 as deduced from its corresponding DNA surrogates. Moreover, non-target miRNAs, as well as single-base mutation targets, can be detected. Overall, the D-CHA strategy provides a competitive method for plant miRNAs detection.
Synthesis and Toxicity Evaluation of New Pyrroles Obtained by the Reaction of Activated Alkynes with 1-Methyl-3-(cyanomethyl)benzimidazolium Bromide
Authors: Ivan Beatrice-Cristina , Dumitrascu Florea , Anghel Adriana Iuliana , Ancuceanu Robert Viorel , Shova Sergiu , Dumitrescu Denisa , Draghici Constantin , Olaru Octavian Tudorel , Nitulescu George Mihai , Dinu Mihaela , Barbuceanu Stefania-Felicia
Published: October 2021

A series of new pyrrole derivatives were designed as chemical analogs of the 1,4-dihydropyridines drugs in order to develop future new calcium channel blockers. The new tri- and tetra-substituted N-arylpyrroles were synthesized by the one-pot reaction of 1-methyl-3-cyanomethyl benzimidazolium bromide with substituted alkynes having at least one electron-withdrawing substituent, in 1,2-epoxybutane, acting both as the solvent and reagent to generate the corresponding benzimidazolium N3-ylide. The structural characterization of the new substituted pyrroles was based on IR, NMR spectroscopy as well as on single crystal X-ray analysis. The toxicity of the new compounds was assessed on the plant cell using Triticum aestivum L. species and on the animal cell using Artemia franciscana Kellogg and Daphnia magna Straus crustaceans. The compounds showed minimal phytotoxicity on Triticum rootlets and virtually no acute toxicity on Artemia nauplii, while on Daphnia magna, it induced moderate to high toxicity, similar to nifedipine. Our research indicates that the newly synthetized pyrrole derivatives are promising molecules with biological activity and low acute toxicity.
Structure and IR Spectroscopic Properties of HNCO Complexes with SO2 Isolated in Solid Argon
Authors: Krupa Justyna , Wierzejewska Maria , Lundell Jan
Published: October 2021

FTIR spectroscopy was combined with the matrix isolation technique and quantum chemical calculations with the aim of studying complexes of isocyanic acid with sulfur dioxide. The structures of the HNCO⋯SO2 complexes of 1:1, 1:2 and 2:1 stoichiometry were optimized at the MP2, B3LYPD3, B2PLYPD3 levels of theory with the 6-311++G(3df,3pd) basis set. Five stable 1:1 HNCO⋯SO2 complexes were found. Three of them contain a weak N-H⋯O hydrogen bond, whereas two other structures are stabilized by van der Waals interactions. The analysis of the HNCO/SO2/Ar spectra after deposition indicates that mostly the 1:1 hydrogen-bonded complexes are present in argon matrices, with a small amount of the van der Waals structures. Upon annealing, complexes of the 1:2 stoichiometry were detected, as well.
Plasmon Waveguide Resonance: Principles, Applications and Historical Perspectives on Instrument Development
Authors: Rascol Estelle , Villette Sandrine , Harté Etienne , Alves Isabel D.
Published: October 2021

Plasmon waveguide resonance (PWR) is a variant of surface plasmon resonance (SPR) that was invented about two decades ago at the University of Arizona. In addition to the characterization of the kinetics and affinity of molecular interactions, PWR possesses several advantages relative to SPR, namely, the ability to monitor both mass and structural changes. PWR allows anisotropy information to be obtained and is ideal for the investigation of molecular interactions occurring in anisotropic-oriented thin films. In this review, we will revisit main PWR applications, aiming at characterizing molecular interactions occurring (1) at lipid membranes deposited in the sensor and (2) in chemically modified sensors. Among the most widely used applications is the investigation of G-protein coupled receptor (GPCR) ligand activation and the study of the lipid environment’s impact on this process. Pioneering PWR studies on GPCRs were carried out thanks to the strong and effective collaboration between two laboratories in the University of Arizona leaded by Dr. Gordon Tollin and Dr. Victor J. Hruby. This review provides an overview of the main applications of PWR and provides a historical perspective on the development of instruments since the first prototype and continuous technological improvements to ongoing and future developments, aiming at broadening the information obtained and expanding the application portfolio.
Nanostructure of PMMA/MAM Blends Prepared by Out-of-Equilibrium (Extrusion) and Near-Equilibrium (Casting) Self-Assembly and Their Nanocellular or Microcellular Structure Obtained from CO2 Foaming
Authors: Barroso-Solares Suset , Bernardo Victoria , Cuadra-Rodriguez Daniel , Pinto Javier
Published: October 2021

Blends of poly(methyl methacrylate) (PMMA) and a triblock copolymer poly(methyl methacrylate)-b-poly(butyl acrylate)-b-poly(methyl methacrylate) (MAM) have been obtained following both out-of-equilibrium (extrusion) and near-equilibrium (solvent casting) production routes. The self-assembly capability and the achievable nanostructures of these blends are analyzed by transmission electron microscopy (TEM) regarding their production route and potential for the achievement of nanocellular foams by CO2 gas dissolution foaming. The influence of the initial nanostructure of the solids on the obtained cellular structure of bulk and film samples is determined by high-resolution scanning electron microscopy (HRSEM) for diverse foaming conditions (saturation pressure, saturation temperature, and post-foaming stage), taking into account the required use of a foaming mold to achieve foams from films. Moreover, the influence of the nanostructuration on the presence of solid outer layers, typical of the selected foaming process, is addressed. Finally, consideration of a qualitative model and the obtained results in terms of nanostructuration, cellular structure, and foaming behavior, allow proposing a detailed cell nucleation, growth, and stabilization scheme for these materials, providing the first direct evidence of the cell nucleation happening inside the poly(butyl acrylate) phase in the PMMA/MAM blends.