bluuebunny/crossref_metadata_2025 huggingface dataset:
Unveiling the Electrocatalytic Performances of the Pd-MoS2 Catalyst for Methanol-Mediated Overall Water Splitting
Authors: Teli Aviraj M. , Mane Sagar M. , Mishra Rajneesh Kumar , Jeon Wookhee , Shin Jae Cheol
Published: January 2025

Herein, this work elucidates the synthesis of the Pd-MoS2 catalyst for application in methanol-mediated overall water splitting. The scanning electron microscope (SEM) and transmission electron microscope (TEM) pictures offer an exciting nanostructured shape of the Pd-MoS2, depicting a high surface area. Further, high-resolution TEM (HRTEM) pictures confirm the lattice plane (100), lattice spacing (0.26 nm), and hexagonal crystal structure of the Pd-MoS2. Moreover, high-angle annular dark-field (HAADF) images and related color maps disclose the Mo, S, and Pd elements of the Pd-MoS2. The Pd-MoS2 catalyst exhibits lower overpotentials of 224.6 mV [methanol-mediated hydrogen evolution reaction (MM-HER)] at −10 mA cm−2 and 133 mV [methanol-mediated oxygen evolution reaction (MM-OER)] at 10 mA cm−2. Further, the Pd-MoS2 illustrates noteworthy stability for 15.5 h for MM-HER and 18 h for MM-OER by chronopotentiometry test. Excitingly, the Pd-MoS2∥Pd-MoS2 cell reveals a small potential of 1.581 V compared to the MoS2∥MoS2 cell (1.648 V) in methanol-mediated overall water splitting. In addition, the Pd-MoS2∥Pd-MoS2 combination reveals brilliant durability over 18 h at 10 mA cm−2.
New Species of Empidinae (Diptera) from San Rossore National Park, Italy, with the First Report on Leg Polymorphism in the Genus Hilara Meigen and Their DNA Barcoding Evidence
Authors: Barták Miroslav , Kozánek Milan , Belcari Antonio , Tóthová Andrea Š.
Published: January 2025

Altogether three species of Empidinae are described from San Rossore National Park, Italy: Empis (Euempis) sanrossorensis Barták sp. nov., Hilara polymorpha Barták sp. nov., and Rhamphomyia (Megacyttarus) sanrossorensis Barták sp. nov. Polymorphism in the shape of foreleg in Hilara is reported for the first time. The COI sequences for barcoding purposes and upcoming studies are provided.
Comparative Efficacy of Extracorporeal Versus Conventional Cardiopulmonary Resuscitation in Adult Refractory Out-of-Hospital Cardiac Arrest: A Retrospective Study at a Single Center
Authors: Lee Juncheol , Jeong Yong Ho , Kim Yun Jin , Cho Yongil , Oh Jaehoon , Jang Hyo Jun , Shin Yonghoon , Kim Ji Eon , Kim Hee Jung , Cho Yang Hyun , Jung Jae Seung , Lee Jun Ho
Published: January 2025

Background: Extracorporeal cardiopulmonary resuscitation (ECPR) has the potential to improve neurological outcomes in patients with refractory out-of-hospital cardiac arrest (OHCA), offering an alternative to conventional cardiopulmonary resuscitation (CCPR). However, its effectiveness in OHCA remains controversial despite advancements in resuscitation techniques. Methods: This retrospective single-center study compared neurological outcomes and 30-day survival between ECPR and CCPR patients from January 2014 to January 2022. Patients aged 18–75 with witnessed OHCA, minimal no flow and low flow times, and cardiac arrests occurring at home or in public places were included. All patients were transported directly to our institution, a tertiary medical center serving the southeastern region of Seoul, where extracorporeal membrane oxygenation implantation was consistently performed in the emergency department. Neurological outcomes were assessed using Cerebral Performance Category scores, with good outcomes defined as scores of 1–2. Statistical analyses included logistic regression models and Kaplan–Meier survival curves, adjusted for confounders using inverse probability of treatment weighting. Results: ECPR was associated with significantly better neurological outcomes than CCPR (p < 0.001). Factors predicting poor outcomes included older age and longer low flow times, while male sex and shockable rhythms were protective. No significant difference was found in 30-day survival between the ECPR and CCPR groups, although a trend toward better survival was noted with ECPR. Conclusions: ECPR may improve neurological outcomes in patients with refractory OHCA compared to CCPR, although it does not significantly affect 30-day survival. Further studies are necessary to validate these findings and explore the long-term impacts of ECPR.
Sleep Quality Among Patients and Healthcare Providers in the Primary Healthcare Setting
Authors: Krasnik Rastislava , Kolundžić Mirjana , Mikov Aleksandra , Zvekić-Svorcan Jelena , Vukliš Dragana , Kovačević Milena , Mikić Andrijana , Mikov Igor , Dedić Novaković Dajana , Stanić Milica
Published: January 2025

Background/Objectives: As adults spend about 30% of each day asleep, having a sleep disorder can negatively affect their functioning. The study objective was to determine the factors that influence sleep quality among patients and healthcare providers in the primary healthcare setting. Methods: This study included respondents of both sexes aged 18–90 years, comprising outpatients and the healthcare providers working in the General Medicine Service at the “Liman” Department of the “Novi Sad” Health Center in Novi Sad, Republic of Serbia. Demographic data along with factors related to lifestyle and sleep habits were collected using a demographic questionnaire specifically designed for this research. The standardized Pittsburgh Sleep Quality Questionnaire, Serbian version (PSQI), was used to assess sleep quality. Results: The study sample comprised 92 respondents (42 healthcare providers and 50 patients), 73.9% of whom were female, with an average age of 50.0 years. Although 50% of healthcare providers reported sleeping only 3–6 h the previous night, according to the PSQI results, patients had lower overall sleep quality (54.0% vs. 33.3%; p = 0.046) and achieved lower scores in the DISTB (sleep disturbances, p = 0.001), SLPQUAL (subjective sleep quality, p = 0.013) and MEDS (use of sleeping medication, p = 0.003) PSQI domains. Conclusions: Sleep quality is impaired in more than half of patients and more than a third of healthcare providers. By detecting and acting on the factors in the home and work environment that affect quality of sleep, and by changing lifestyle habits, sleep quality in adults can be improved.
Analysis of the Feasibility of the OrthoNail Hybrid Intramedullary Implant in the Human Body with Respect to Material Durability
Authors: Grygier Dominika , Kowalewski Piotr , Opałka Mariusz , Słowiński Jakub J. , Dziubek Mateusz , Pyka Dariusz
Published: January 2025

This study focuses on the development and evaluation of the OrthoNail hybrid intramedullary implant for lower limb lengthening in patients requiring significant skeletal reconstruction. The implant addresses the challenges in load-bearing during rehabilitation, providing a robust solution that is capable of supporting physiological loads. Mechanical tests, including axial compression, tension, torsion, and 3,4-point bending, determined the implant’s load capacity and fatigue resistance, while finite element analysis assessed stress distributions in bone tissue and around screw holes during single-leg stance, with boundary conditions derived from Orthoload database data. The OrthoNail implant demonstrated excellent mechanical stability, sustaining torsional loads of up to 19.36 Nm at maximum elongation (80 mm) and 17.16 Nm at zero elongation. Under axial compression, it withstood forces of up to 1400 N, maintaining structural integrity. Fatigue testing revealed resilience under dynamic loading conditions for over 1,000,000 cycles at a load of 500 N, with no mechanical failure or material degradation observed. Stress concentrations near screw holes indicate areas for potential optimization. The findings indicate that the OrthoNail implant demonstrates excellent mechanical stability and is well-suited for clinical application, enabling early full weight-bearing during rehabilitation.
Investigation of Digital Light Processing-Based 3D Printing for Optimized Tooling in Automotive and Electronics Sheet Metal Forming
Authors: Szalai Szabolcs , Szívós Brigitta Fruzsina , Nemes Vivien , Szabó György , Kurhan Dmytro , Sysyn Mykola , Fischer Szabolcs
Published: January 2025

This study addresses the emerging need for efficient and cost-effective solutions in low-volume production by exploring the mechanical performance and industrial feasibility of cutting tools that are fabricated using stereolithography apparatus (SLA) technology. SLA’s high-resolution capabilities make it suitable for creating precise cutting dies, which were tested on aluminum sheets (Al99.5, 0.3 mm, and AlMg3, 1.0 mm) under a 60-ton hydraulic press. Measurements using digital image correlation (DIC) revealed minimal wear and deformation, with tolerances consistently within IT 0.1 mm. The results demonstrated that SLA-printed tools perform comparably to conventional metal tools in cutting and bending operations, achieving similar surface quality and edge precision while significantly reducing the production time and cost. Despite some limitations in wear resistance, the findings highlight SLA technology’s potential for rapid prototyping and short-run manufacturing in the automotive and electronics sectors. This research fills a critical gap in understanding SLA-based tooling applications, offering insights into process optimization to enhance tool durability and broaden material compatibility. These advancements position SLA technology as a transformative tool-making technology for flexible manufacturing.
Codesigning More-than-Human Ecosystems with Social and Environmental Systems: The Gamification of NetWall and BioDiveIn
Authors: Davidová Marie , Valverde Rojas María Claudia , Behnam Hanane
Published: January 2025

This study explores the integration of gamification into social and environmental systems to enhance urban biodiversity and foster the co-creation of ecosystems. It focuses on two key contributions: the development of tangible ecosystemic interventions, such as habitat extensions and edible landscapes, and the gamification of these interventions to engage communities. The interventions were codesigned using systems-oriented design methods, including gigamapping and prototyping, to produce scalable DIY solutions that empower communities to replicate these practices on their own. Additionally, urban games were created to incentivize participation by rewarding individuals for their contributions to biodiversity restoration.
A New Pyrrole Alkaloid from Capsicum annuum L. var. palmera Grown in La Palma (Canary Islands, Spain)
Authors: Díaz Jesús G. , de Buergo María A. , Díaz David Díaz
Published: January 2025

Capsicum annuum, commonly known as pepper, is a widely cultivated crop valued for its nutritional and economic significance. Among its varieties, the small red bell pepper, locally known as “pimienta palmera”, is a unique cultivar from La Palma in the Canary Islands, renowned for its distinctive flavor and use in traditional dishes. Despite its cultural importance, limited research exists on the chemical composition of this pepper variety. This study investigates the chemical constituents of dichloromethane and n-butanol fractions extracted from the pericarp of Capsicum annuum var. palmera. Isolation and structural elucidation of compounds were achieved using high-resolution mass spectrometry (HREIMS), nuclear magnetic resonance spectroscopy (NMR), and chromatographic techniques. A previously unreported pyrrolo-lactone compound, 2-(6-formyl-3-oxo-3,4-dihydro-1H-pyrrolo[2,1-c][1,4]oxazin-4-yl)acetic acid, was identified alongside other metabolites, including α-tocopherol, β-sitosterol, and oleic acid. This alkaloid adds to the chemical diversity of Capsicum species and highlights the unique bioactive potential of this regional pepper. These findings enhance the understanding of pimienta palmera’s composition and suggest potential applications in nutraceuticals and pharmacology due to the biological activity associated with pyrrole alkaloids. The characterization of this compound underscores the need to further explore the phytochemical richness of traditional crops with limited prior research.
3-Benzoyl-1′,3′,6-trimethyl-2′H,3H,4H-spiro[furo[3,2-c]pyran-2,5′-pyrimidine]-2′,4,4′,6′(1′H,3′H)-tetraone
Authors: Elinson Michail N. , Kalashnikova Varvara M. , Ryzhkova Yuliya E. , Rakitin Oleg A.
Published: January 2025

We describe an approach towards the synthesis of previously unknown 3-benzoyl-1′,3′,6-trimethyl-2′H,3H,4H-spiro[furo[3,2-c]pyran-2,5′-pyrimidine]-2′,4,4′,6′(1′H,3′H)-tetraone. The presented method is based on the cyclization of 5-(1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)-2-oxo-2-phenylethyl)-1,3-dimethylpyrimidine-2,4,6(1H,3H,5H)-trione. It was shown that the presented reaction proceeds through the stage of bromination of the starting compound followed by O-nucleophilic attack. The structures of the obtained compound were established by 1H, 13C NMR and IR spectroscopy, and high-resolution mass spectrometry.
Improving Electrical Conductivity of Commercially Pure Aluminium: The Synergistic Effect of AlB8 Master Alloy and Heat Treatment
Authors: Zeybek Yusuf , Kayış Cemile , Diler Ege Anıl
Published: January 2025

This study aims to enhance the electrical conductivity of commercially pure aluminium by minimizing impurities and grain boundaries in its microstructure, ultimately improving the efficiency of electric motors constructed from rotors with squirrel cages made from this material. For this purpose, an aluminium–boron (AlB8) master alloy was added to aluminium with a purity of 99.7%, followed by the application of a grain-coarsening heat treatment to the rotors. To obtain commercially pure aluminium with boron additions of 0.05% and 0.1% by weight, specific amounts of the AlB8 master alloy were added into aluminium with a purity of 99.7%. Using these materials, squirrel cage components of rotors were produced via the high-pressure die-casting method. Subsequently, a grain-coarsening heat treatment of the rotors was performed at temperatures of 450 °C, 500 °C, and 550 °C, with holding times of 2, 6, and 10 h. The Box–Behnken design, which is based on statistical experimental design and response surface methodology, was employed to investigate the effects of adding boron and varying the heat treatment temperature and holding time on the electrical conductivity of commercially pure aluminium. The results showed that the synergistic effect of adding boron at 0.05 wt.% and applying the grain-coarsening heat treatment at a temperature of 550 °C for a holding time of 10 h significantly enhanced the electrical conductivity of commercially pure aluminium, increasing it from 60.62% IACS to 63.1% IACS. Correspondingly, the efficiency of the electric motor increased from 90.35% to 91.53%. These findings suggest that this hybrid method not only enhances the electrical conductivity of commercially pure aluminium but also has strong potential to improve its other properties, such as thermal conductivity. This will lead to products composed of components manufactured from the materials exhibiting better performance characteristics, such as increased efficiency and extended service life. Consequently, this innovative method will contribute economically and environmentally by facilitating the manufacture of high-performance products.
Numerical Study to Analyze the Influence of Process Parameters on Temperature and Stress Field in Powder Bed Fusion of Ti-6Al-4V
Authors: Mohammadkamal Helia , Caiazzo Fabrizia
Published: January 2025

This paper presents a comprehensive numerical investigation to simulate heat transfer and residual stress formation of Ti-6Al-4V alloy during the Laser Powder Bed Fusion process, using a finite element model (FEM). The FEM was developed with a focus on the effects of key process parameters, including laser scanning velocity, laser power, hatch space, and scanning pattern in single-layer scanning. The model was validated against experimental data, demonstrating good agreement in terms of temperature profiles and melt pool dimensions. The study elucidates the significant impact of process parameters on thermal gradients, melt pool characteristics, and residual stress distribution. An increase in laser velocity, from 600 mm/s to 1500 mm/s, resulted in a smaller melt pool area and faster cooling rate. Similarly, the magnitude of residual stress initially decreased and subsequently increased with increasing laser velocity. Higher laser power led to an increase in melt pool size, maximum temperature, and thermal residual stress. Hatch spacing also exhibited an inverse relationship with thermal gradient and residual stress, as maximum residual stress decreased by about 30% by increasing the hatch space from 25 µm to 75 µm. The laser scanning pattern also influenced the thermal gradient and residual stress distribution after the cooling stage.
HTTD: A Hierarchical Transformer for Accurate Table Detection in Document Images
Authors: Kasem Mahmoud SalahEldin , Mahmoud Mohamed , Yagoub Bilel , Senussi Mostafa Farouk , Abdalla Mahmoud , Kang Hyun-Soo
Published: January 2025

Table detection in document images is a challenging problem due to diverse layouts, irregular structures, and embedded graphical elements. In this study, we present HTTD (Hierarchical Transformer for Table Detection), a cutting-edge model that combines a Swin-L Transformer backbone with advanced Transformer-based mechanisms to achieve superior performance. HTTD addresses three key challenges: handling diverse document layouts, including historical and modern structures; improving computational efficiency and training convergence; and demonstrating adaptability to non-standard tasks like medical imaging and receipt key detection. Evaluated on benchmark datasets, HTTD achieves state-of-the-art results, with precision rates of 96.98% on ICDAR-2019 cTDaR, 96.43% on TNCR, and 93.14% on TabRecSet. These results validate its effectiveness and efficiency, paving the way for advanced document analysis and data digitization tasks.
The Impact of Targeted Therapies on Red Blood Cell Aggregation in Patients with Chronic Lymphocytic Leukemia Evaluated Using Software Image Flow Analysis
Authors: Alexandrova-Watanabe Anika , Abadjieva Emilia , Gartcheva Lidia , Langari Ariana , Ivanova Miroslava , Guenova Margarita , Tiankov Tihomir , Strijkova Velichka , Krumova Sashka , Todinova Svetla
Published: January 2025

Chronic lymphocytic leukemia (CLL), the most common type of leukemia, remains incurable with conventional therapy. Despite advances in therapies targeting Bruton’s tyrosine kinase and anti-apoptotic protein BCL-2, little is known about their effect on red blood cell (RBC) aggregation in blood flow. In this study, we applied a microfluidic device and a newly developed Software Image Flow Analysis to assess the extent of RBC aggregation in CLL patients and to elucidate the hemorheological effects of the commonly applied therapeutics Obinutuzumab/Venetoclax and Ibrutinib. The results revealed that, in RBC samples from untreated CLL patients, complex 3D clusters of large RBC aggregates are formed, and their number is significantly increased compared to healthy control samples. The application of the Obinutuzumab/Venetoclax combination did not affect this aspect of RBCs’ rheological behavior. In contrast, targeted therapy with Ibrutinib preserves the aggregation state of CLL RBCs to levels seen in healthy controls, demonstrating that Ibrutinib mitigates the alterations in the rheological properties of RBCs associated with CLL. Our findings highlight the alterations in RBC aggregation in CLL and the impact of different targeted therapies on RBCs’ rheological properties, which is critical for predicting the potential complications and side effects of CLL treatments, particularly concerning blood flow dynamics.
Exploring Metal Ions as Potential Antimicrobial Agents to Combat Future Drug Resistance in Mycoplasma bovis
Authors: Alkhallawi Mauida F. Hasoon , Mohammed Majed H. , Hemmatzadeh Farhid , Petrovski Kiro
Published: January 2025

The rise in antimicrobial resistance (AMR) in Mycoplasma bovis underscores the urgent need for alternative treatments. This study evaluated the minimal inhibitory concentrations (MICs) of four metal ions (cobalt, copper, silver, and zinc) and colloidal silver against 15 clinical M. bovis isolates, alongside conventional antimicrobials (florfenicol, tetracycline, tulathromycin, and tylosin). Colloidal silver demonstrated the most effective antimicrobial activity, inhibiting 81.25% of isolates at 1.5 mg/L, while silver inhibited 93.7% of isolates at concentrations above 1.5 mg/L. Copper exhibited notable efficacy, inhibiting 37.5% of isolates at 1.5 mg/L, with a small proportion responding at 0.1 mg/L. Cobalt and zinc displayed variable activity, with MIC values ranging from 0.7 to 12.5 mg/L. In contrast, conventional antimicrobials showed limited effectiveness: tetracycline inhibited 31.25% of isolates at ≥16 mg/L, tylosin inhibited 25% at 16 mg/L, and tulathromycin MICs ranged from 0.5 to 8 mg/L. Time–kill assays revealed a reduction in M. bovis viability after eight hours of exposure to silver and colloidal silver, though higher concentrations (4×–8× MIC) were required for complete eradication. These findings highlight the significant potential of colloidal silver and copper as alternatives for treating M. bovis infections and combating AMR. Further research is essential to explore their standalone and synergistic applications for therapeutic use.
The Role and the Regulation of NLRP3 Inflammasome in Irritable Bowel Syndrome: A Narrative Review
Authors: Kasti Arezina , Katsas Konstantinos , Nikolaki Maroulla D. , Triantafyllou Konstantinos
Published: January 2025

Irritable bowel syndrome (IBS) is a chronic disorder of the gastrointestinal tract. Its pathogenesis involves multiple factors, including visceral hypersensitivity and immune activation. NLRP3 inflammasome is part of the nucleotide-binding oligomerization domain-like receptor (NLR) family, a crucial component of the innate immune system. Preclinical studies have demonstrated that inhibiting NLRP3 reduces visceral sensitivity and IBS symptoms, like abdominal pain, and diarrhea, suggesting that targeting the NLRP3 might represent a novel therapeutic approach for IBS. This review aims to assess the NLRP3 inhibitors (tranilast, β-hydroxybutyrate, Chang-Kang-fang, paeoniflorin, coptisine, BAY 11-7082, and Bifidobacterium longum), highlighting the signaling pathways, and their potential role in IBS symptoms management was assessed. Although premature, knowledge of the action of synthetic small molecules, phytochemicals, organic compounds, and probiotics might make NLRP3 a new therapeutic target in the quiver of physicians’ therapeutic choices for IBS symptoms management.
Halophilic Fungi—Features and Potential Applications
Authors: Yovchevska Lyudmila , Gocheva Yana , Stoyancheva Galina , Miteva-Staleva Jeny , Dishliyska Vladislava , Abrashev Radoslav , Stamenova Tsvetomira , Angelova Maria , Krumova Ekaterina
Published: January 2025

Extremophiles are of significant scientific interest due to their unique adaptation to harsh environmental conditions and their potential for diverse biotechnological applications. Among these extremophiles, filamentous fungi adapted to high-salt environments represent a new and valuable source of enzymes, biomolecules, and biomaterials. While most studies on halophiles have focused on bacteria, reports on filamentous fungi remain limited. This review compiles information about salt-adapted fungi and details their distribution, adaptation mechanisms, and potential applications in various societal areas. Understanding the adaptive mechanisms of halophilic fungi not only sheds light on the biology of extremophilic fungi but also leads to promising biotechnological applications, including the development of salt-tolerant enzymes and strategies for bioremediation of saline habitats. To fully realize this potential, a comprehensive understanding of their ecology, diversity and physiology is crucial. In addition, understanding their survival mechanisms in saline environments is important for the development of astrobiology. The significant potential of applications of halophilic fungi is highlighted.
Synthesis and Biological Evaluation of New cis-Restricted Triazole Analogues of Combretastatin A-4
Authors: Prieto Lidia , Gaviña Daniel , Escolano Marcos , Cánovas-Belchí María , Sánchez-Roselló María , del Pozo Carlos , Falomir Eva , Díaz-Oltra Santiago
Published: January 2025

The natural products combretastatins A-1 and A-4 are potent antimitotic and vascular-disrupting agents through their binding at the colchicine site in tubulin. However, these compounds suffer from a low water solubility and a tendency to isomerize to the inactive trans stilbenes. In this study, we have prepared a series of 18 cis-restricted triazole analogues of combretastatin A-4 (CA-4), maintaining, in all cases, the 3,4,5-trimethoxy phenyl ring A, with the aim of investigating the substitution pattern on the B-ring in a systematic way. To this end, cytotoxic activities of the cis-restricted analogues of CA-4 prepared were determined in two tumor cell lines, namely, HT-29 and A-549, as well as in the non-tumor cell line HEK-293, to pre-evaluate the selectivity profile of the compounds for the tumor cell lines. The main conclusion was the essential presence of methoxyl or ethoxyl groups at the para position of the B-ring in order to obtain good antitumor activities. Thus, the more active compounds in our study displayed IC50 values in the nanomolar range for the tumor cell lines but not for the normal cells. Consequently, these triazole analogues of CA-4 could serve as promising alternatives to the natural product, although further studies about their biological activity are essential in order to fully determine their viability as therapeutic agents in the treatment of cancer.
A Guinea Pig Model of Pediatric Metabolic Dysfunction-Associated Steatohepatitis: Poor Vitamin C Status May Advance Disease
Authors: Pedersen Kamilla , Poojari Ankita , Colberg Simone Frederikke , Mechernsee Stine Marguerite , Iversen Jo Frøkjær , Barrès Romain , Lykkesfeldt Jens , Tveden-Nyborg Pernille
Published: January 2025

Background/Objectives: Children and teenagers display a distinct metabolic dysfunction-associated steatohepatitis (MASH) phenotype, yet studies of childhood MASH are scarce and validated animal models lacking, limiting the development of treatments. Poor vitamin C (VitC) status may affect MASH progression and often co-occurs with high-fat diets and related metabolic imbalances. As a regulator of DNA methylation, poor VitC status may further contribute to MASH by regulating gene expression This study investigated guinea pigs—a species that, like humans, depends on vitC in the diet—as a model of pediatric MASH, examining the effects of poor VitC status on MASH hallmarks and global DNA methylation levels. Methods: Sixty-two juvenile guinea pigs were exposed to a high-fat diet for 16 weeks. Results: Juvenile guinea pigs exhibited hepatic histopathology representative of pediatric MASH, confirmed by portal inflammation and fibrosis. Consistent with pediatric MASH, juvenile guinea pigs displayed increased lobular and portal inflammation (p < 0.05 and p < 0.0001, respectively) but less steatosis (p < 0.001) compared to adults. Compared to the controls, the guinea pigs deprived in VitC showed lower body weight (p < 0.01), higher expression of hepatic inflammatory genes (p < 0.05), and a lower global hydroxymethylcytosine to methylcytosine ratio in the high-fat groups (p < 0.05). Conclusions: Our study validates guinea pigs as a model of pediatric MASH and suggests that VitC contributes to an altered gene expression signature through the regulation of DNA hydroxymethylation. We postulate that nutritional co-deficiencies in MASH, such as low VitC, may accelerate disease progression and deserve further attention.
The Anthelmintic Activity of Nepeta racemosa Lam. Against Gastrointestinal Nematodes of Sheep: Rosmarinic Acid Quantification and In Silico Tubulin-Binding Studies
Authors: Ağören Büşra Karpuz , Erez Mahmut Sinan , Kozan Esma , Dağyaran Aydın , Akdağ Mevlüt , Sobarzo-Sánchez Eduardo , Küpeli Akkol Esra
Published: January 2025

Gastrointestinal nematodes (GINs) inflict significant economic losses on sheep and goat farming globally due to reduced productivity and the development of anthelmintic resistance. Sustainable control strategies are urgently needed including the exploration of medicinal plants as safer alternatives to chemical anthelmintics. This genus of plants is used for anti-inflammatory, antioxidant, and antimicrobial activities. In this study, we aimed to evaluate the anthelmintic activities of Nepeta racemosa Lam. MeOH extract, n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc), n-buthanol (n-BuOH) and aqueous (H2O) subextracts, and quantify rosmarinic acid in the active extract by the HPLC method, and perform in silico molecular docking studies of rosmarinic acid to examine its binding interactions with tubulin. The anthelmintic activity of the plant extracts on gastrointestinal nematode eggs and larvae (L3) of the sheep was assessed using in vitro test methods such as the egg hatch assay and larval motility assay, conducted over a 24 h period (1, 2, 3, 4, 6, 8, 24). All extracts exhibited 100% effectiveness in the egg hatch inhibition assay, regardless of concentration (50–1.5625 mg/mL). The EtOAc subextract shows the highest effectiveness at 79.66%, followed by the MeOH extract at 74.00%, water at 64.00%, n-hexane at 67.00%, and DCM at 61.00%, and the lowest effectiveness is observed with n-BuOH at 51.66% in the larval motility assay. The major compound of EtOAc extract, the most active extract of N. racemosa, was determined as rosmarinic acid and its amount in the extract was determined as 14.50 mg/100 mg dry extract. The amount of rosmarinic acid in the MeOH extract was found to be 0.21 mg/100 mg dry extract. n-Hexane, DCM, n-BuOH, and H2O extracts’ rosmarinic acid content was lower than the LOQ value. As tubulin plays an important role in the mechanism of anthelmintics, the major compound of the most active extract (NR-EtOAc) rosmarinic acid was docked onto the colchicine-binding site of the tubulin (5OV7) protein. Rosmarinic acid showed a similar activity spectrum to the anthelmintic drug albendazole. The discovery of low-cost and low-toxicity anthelmintic compounds is very important.
Antibiotic Susceptibility Patterns and Virulence Profiles of Classical and Hypervirulent Klebsiella pneumoniae Strains Isolated from Clinical Samples in Khyber Pakhtunkhwa, Pakistan
Authors: Azra  , Khan Taj Ali , Ul Haq Ihtisham , Hinthong Woranich , Campino Susana , Gohar Aisha , Khan Noman , Kashif Muhammad , Ullah Ihsan , Clark Taane G.
Published: January 2025

The emergence of hypervirulent and carbapenem-resistant hypermucoviscous Klebsiella pneumoniae strains presents a significant public health challenge due to their increased virulence and resistance to multiple antibiotics. This study evaluates the antibiotic susceptibility patterns and virulence profiles of classical and hypervirulent K. pneumoniae strains isolated from various clinical samples. A total of 500 clinical samples were collected from patients at the Mardan Medical Complex and Ayub Medical Complex in KPK between July 2022 and June 2024. Among these, 64 K. pneumoniae strains were isolated and subsequently subjected to antimicrobial susceptibility testing (AST) and phenotypic virulence detection. Among the 64 isolates, 21 (32.8%) exhibited hypermucoviscosity, a characteristic associated with increased pathogenicity. Hemagglutination was observed in 35 (54.1%) of the isolates, indicating the presence of surface adhesins that facilitate bacterial adherence to host tissues. A high prevalence of biofilm formation was noted, with 54 (84%) isolates capable of forming biofilms, which are known to protect bacteria from antibiotics and the host immune response. Most isolates (59/64, 92.1%) were resistant against ampicillin, highlighting its limited efficacy against these strains. Conversely, the lowest resistance was observed for tigecycline, with only 15% (10/64) of the isolates showing resistance, indicating its potential utility as a treatment option. The study also found that 38 (59.3%) of the isolates were extended-spectrum beta-lactamase (ESBL) producers, 42 (65.6%) were multidrug-resistant (MDR), 32 (50%) were extensively drug-resistant (XDR), and 13 (20.3%) were resistant to carbapenems. The genetic study revealed biofilm producer and enhancer genes (mrkD, pgaABCD, fimH, treC, wzc, pilQ, and luxS) mainly in the hypervirulent strains. These hypervirulent strains also show a high number of resistance genes. The findings of this study underscore the critical need for the active surveillance of antimicrobial resistance and virulence determinants in K. pneumoniae. The coexistence of high levels of antibiotic resistance and virulence factors in these isolates poses a severe threat to public health, as it can lead to difficult-to-treat infections and increased morbidity and mortality.
Nano-Zinc Oxide Can Enhance the Tolerance of Apple Rootstock M9-T337 Seedlings to Saline Alkali Stress by Initiating a Variety of Physiological and Biochemical Pathways
Authors: Zhai Jietao , Xian Xulin , Zhang Zhongxing , Wang Yanxiu
Published: January 2025

Soil salinization severely restricts the growth and development of crops globally, especially in the northwest Loess Plateau, where apples constitute a pillar industry. Nanomaterials, leveraging their unique properties, can facilitate the transport of nutrients to crops, thereby enhancing plant growth and development under stress conditions. To investigate the effects of nano zinc oxide (ZnO NP) on the growth and physiological characteristics of apple self-rooted rootstock M9-T337 seedlings under saline alkali stress, one-year-old M9-T337 seedlings were used as experimental materials and ZnO NPs were used as donors for pot experiment. Six treatments were set up: CK (normal growth), SA (saline alkali stress,100 mmol/L NaCl + NaHCO3), T1 (saline alkali stress + 50 mg/L ZnO NPs), T2 (saline alkali stress + 100 mg/L ZnO NPs), T3 (saline alkali stress + 150 mg/L ZnO NPs) and T4 (saline alkali stress + 200 mg/L ZnO NPs). The results were found to show that saline alkali stress could significantly inhibit the growth and development of M9-T337 seedlings, reduce photosynthetic characteristics, and cause ion accumulation to trigger osmotic regulation system, endogenous hormone and antioxidant system imbalances. However, the biomass, plant height, stem diameter, total leaf area and leaf perimeter of M9-T337 seedlings were significantly increased after ZnO NP treatment. Specifically speaking, ZnO NPs can improve the photosynthetic capacity of M9-T337 by increasing the content of photosynthetic pigment, regulating photosynthetic intensity and chlorophyll fluorescence parameters. ZnO NPs can balance the osmotic adjustment system by increasing the contents of soluble protein (SP), soluble sugar (SS), proline (Pro) and starch, and can also enhance the activities of enzymatic (SOD, POD, and CAT) and non-enzymatic antioxidant enzymes (APX, AAO, GR, and MDHAR) to enhance the scavenging ability of reactive oxygen species (H2O2, O2•−), ultimately reducing oxidative damage; ZnO NPs promoted the growth of M9-T337 seedlings under saline alkali stress by synergistically responding to auxin (IAA), gibberellin (GA3), zeatin (ZT) and abscisic acid (ABA). Additionally, the Na+/K+ ratio was reduced by upregulating the expression of Na+ transporter genes (MdCAX5, MdCHX15, MdSOS1, and MdALT1) and downregulating the expression of K+ transporter genes (MdSKOR and MdNHX4). After comprehensive analysis of principal components and correlation, T3 (150 mg/L ZnO NPs) treatment possessed the best mitigation effect. In summary, 150 mg/L ZnO NPs(T3) can effectively maintain the hormone balance, osmotic balance and ion balance of plant cells by promoting the photosynthetic capacity of M9-T337 seedlings, and enhance the antioxidant defense mechanism, thereby improving the saline alkaline tolerance of M9-T337 seedlings.
A Combination of Classification Robust Adaptive Kalman Filter with PPP-RTK to Improve Fault Detection for Integrity Monitoring of Autonomous Vehicles
Authors: Elsayed Hassan , El-Mowafy Ahmed , Allahvirdi-Zadeh Amir , Wang Kan , Mi Xiaolong
Published: January 2025

Real-time integrity monitoring (IM) is essential for autonomous vehicle positioning, requiring high availability and manageable computational load. This research proposes using precise point positioning real-time kinematic (PPP-RTK) as the positioning method, combined with an improved classification adaptive Kalman filter (CAKF) for processing. PPP-RTK enhances IM availability by allowing undifferenced and uncombined observations, enabling individual observation exclusion during fault detection and exclusion (FDE). The CAKF reduces FDE computational load by using a robustness test instead of traditional FDE methods, improving precision and availability in protection level estimation. Epoch-wise weighting adjustments in the robustness test create a more accurate stochastic model, aided by an adaptive unit weight variance (UWV) calculated with a sliding window, achieving a 7–28% UWV reduction. Three test scenarios with up to four simultaneous faults in code and phase observations, ranging from 1 to 200 m and 0.4 to 20 m, respectively, demonstrated successful identification and de-weighting of faults, resulting in maximum positioning errors of 6 mm (horizontal) and 11 mm (vertical). The method reduced FDE computational load by 50–99.999% compared to other approaches.
SVDDD: SAR Vehicle Target Detection Dataset Augmentation Based on Diffusion Model
Authors: Wang Keao , Pan Zongxu , Wen Zixiao
Published: January 2025

In the field of target detection using synthetic aperture radar (SAR) images, deep learning-based supervised learning methods have demonstrated outstanding performance. However, the effectiveness of deep learning methods is largely influenced by the quantity and diversity of samples in the dataset. Unfortunately, due to various constraints, the availability of labeled image data for training SAR vehicle detection networks is quite limited. This scarcity of data has become one of the main obstacles hindering the further development of SAR vehicle detection. In response to this issue, this paper collects SAR images of the Ka, Ku, and X bands to construct a labeled dataset for training Stable Diffusion and then propose a framework for data augmentation for SAR vehicle detection based on the Diffusion model, which consists of a fine-tuned Stable Diffusion model, a ControlNet, and a series of methods for processing and filtering images based on image clarity, histogram, and an influence function to enhance the diversity of the original dataset, thereby improving the performance of deep learning detection models. In the experiment, the samples we generated and screened achieved an average improvement of 2.32%, with a maximum of 6.6% in mAP75 on five different strong baseline detectors.
Surveying Nearshore Bathymetry Using Multispectral and Hyperspectral Satellite Imagery and Machine Learning
Authors: Hartmann David , Gravey Mathieu , Price Timothy David , Nijland Wiebe , de Jong Steven Michael
Published: January 2025

Nearshore bathymetric data are essential for assessing coastal hazards, studying benthic habitats and for coastal engineering. Traditional bathymetry mapping techniques of ship-sounding and airborne LiDAR are laborious, expensive and not always efficient. Multispectral and hyperspectral remote sensing, in combination with machine learning techniques, are gaining interest. Here, the nearshore bathymetry of southwest Puerto Rico is estimated with multispectral Sentinel-2 and hyperspectral PRISMA imagery using conventional spectral band ratio models and more advanced XGBoost models and convolutional neural networks. The U-Net, trained on 49 Sentinel-2 images, and the 2D-3D CNN, trained on PRISMA imagery, had a Mean Absolute Error (MAE) of approximately 1 m for depths up to 20 m and were superior to band ratio models by ~40%. Problems with underprediction remain for turbid waters. Sentinel-2 showed higher performance than PRISMA up to 20 m (~18% lower MAE), attributed to training with a larger number of images and employing an ensemble prediction, while PRISMA outperformed Sentinel-2 for depths between 25 m and 30 m (~19% lower MAE). Sentinel-2 imagery is recommended over PRISMA imagery for estimating shallow bathymetry given its similar performance, much higher image availability and easier handling. Future studies are recommended to train neural networks with images from various regions to increase generalization and method portability. Models are preferably trained by area-segregated splits to ensure independence between the training and testing set. Using a random train test split for bathymetry is not recommended due to spatial autocorrelation of sea depth, resulting in data leakage. This study demonstrates the high potential of machine learning models for assessing the bathymetry of optically shallow waters using optical satellite imagery.
Nutritional and Industrial Insights into Hemp Seed Oil: A Value-Added Product of Cannabis sativa L.
Authors: Mygdalia Aggeliki , Panoras Ioannis , Vazanelli Eirini , Tsaliki Eleni
Published: January 2025

Industrial hemp is mainly cultivated for its fibers aimed at the production of textiles, paper, and cordage; the inflorescences for medicinal purposes; and the seeds are used by the food industry due to their high nutritional and functional matrix of protein, fiber, lipids, and microelements. Hemp seed oil (HsO) is a unique source of polyunsaturated fatty acids, with a phenomenal ω6:ω3 ratio of 2.5–3.0, significantly enhancing human health when consumed daily. HsO is mostly obtained through cold pressing due to minimal thermal treatment, and although of lower yield compared to solvent extraction, it presents higher quality lipid fractions and organoleptic characteristics such as color, taste, flavor, and density. Although HsO is a powerful source of polyunsaturated fatty acids, antioxidants, and phytosterols, its production lacks standardized quality control parameters, except for THC, which is subject to EU legislation. Therefore, it is essential to build up a quality protocol system for standardizing seed conservation, oil extraction methods, and quality parameters. This review aims to display an overall nutritional framework of the HsO and encourage further research into its use in the food value chain.
Enhanced Ciprofloxacin Ozonation Degradation by an Aqueous Zn-Cu-Ni Composite Silicate: Degradation Performance and Surface Mechanism
Authors: Liu Yue , Guo Rong , Li Jie , Cheng Yizhen , Wang Congmin , Wang Weiqiang , Zheng Huifan
Published: January 2025

This study investigates the environmental significance of ciprofloxacin as an emerging contaminant and the need for effective degradation methods. The chemical coprecipitation method was used in this study to prepare the Zn-Cu-Ni composite silicate, serving as a heterogeneous ozonation catalyst. The catalytic activity was then evaluated by degrading ciprofloxacin (CIP). Scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, nitrogen adsorption–desorption, and Fourier transform infrared analysis (FTIR) were used to characterize the Zn-Cu-Ni composite silicate. The catalyst had a high surface area (308.137 m2/g), no regular morphology, and a particle size of 7.6 µm and contained Si-O-Si, Ni-O-Si, and Zn-O-Si. The results showed that the CIP degradation and mineralization rates (pH 7.0, CIP 3.0 mg/L, Ozone 1.5 mg/L) were significantly enhanced in the presence of the Zn-Cu-Ni composite silicate. The CIP and total organic carbon (TOC) removal rates were increased by 51.09% and 18.72%, respectively, under optimal conditions, compared with ozonation alone. The adsorption of Zn-Cu-Ni composite silicate, ozone oxidation, and ·OH oxidation synergistically promoted the efficient removal of CIP. This study provides valuable catalytic ozone technology for degradation of antibiotics in wastewater to reduce environmental pollution with potential practical applications.
Perceptions of Fairness of Support Between Older Parents and Adult Children
Authors: Willems Anna , Mortelmans Dimitri , Vercruyssen Anina
Published: January 2025

Increased life expectancy and reduced fertility mean more generations are living simultaneously but with fewer members. There is also a growing group of older people (aged 80 and over) who need care and support. This impacts mutual support within families and the care provided by public or private care organisations. Across OECD countries, on average, 60% of people aged 65 years and older in 2020 reported receiving support from family members, friends and people in their social network, living inside or outside their household but not care organisations. European research shows that when older persons do not have a partner (anymore), they rely on their adult children for care and support. Given that adult children frequently serve as primary providers of informal care, our study examines their perspectives and motivations to provide future care alongside the demands and expectations of their old parents. Our study adopts a multi-actor interview approach and simultaneously looks at the perspective of 40 adult children and one of their older aged parents (65 years or older). We apply the distributive justice theory to understand how children and parents assess the expectation and fairness of support. This paper contributes to the existing literature about support behaviour between parents and children, expanding insights about the fairness of support, expectations and willingness from a multi-actor approach. Through the lens of child–parent dyads, it is seen that the principles of the distributive justice theory can be perceived as not so strict, and within family relationships, one or more principles can coexist and have underlying mechanisms. This study shows the complexity and often ambivalence of family solidarity by adopting a multi-actor approach. One of the main findings is that contrasting dyads who reject the reciprocal act of support experience feelings of guilt or misunderstanding, resulting in stress and worry. A child may not follow the expected support pattern from the parent due to competing demands such as work or the prioritisation of young children, which can reduce the support given to the older parent. Besides general contrasts and similarities between child–parent support perspectives, the analysis looked into differences regarding gender and legal relationships. Our findings only found gendered care expectations. Future research should entangle this by looking into feelings of closeness, emotional connection and considering the dynamic character of filial support over time, especially between siblings.