bluuebunny/crossref_metadata_2025 huggingface dataset:
Can We Treat Neuroinflammation in Alzheimer’s Disease?
Authors: Sánchez-Sarasúa Sandra , Fernández-Pérez Iván , Espinosa-Fernández Verónica , Sánchez-Pérez Ana María , Ledesma Juan Carlos
Published: November 2020

Alzheimer’s disease (AD), considered the most common type of dementia, is characterized by a progressive loss of memory, visuospatial, language and complex cognitive abilities. In addition, patients often show comorbid depression and aggressiveness. Aging is the major factor contributing to AD; however, the initial cause that triggers the disease is yet unknown. Scientific evidence demonstrates that AD, especially the late onset of AD, is not the result of a single event, but rather it appears because of a combination of risk elements with the lack of protective ones. A major risk factor underlying the disease is neuroinflammation, which can be activated by different situations, including chronic pathogenic infections, prolonged stress and metabolic syndrome. Consequently, many therapeutic strategies against AD have been designed to reduce neuro-inflammation, with very promising results improving cognitive function in preclinical models of the disease. The literature is massive; thus, in this review we will revise the translational evidence of these early strategies focusing in anti-diabetic and anti-inflammatory molecules and discuss their therapeutic application in humans. Furthermore, we review the preclinical and clinical data of nutraceutical application against AD symptoms. Finally, we introduce new players underlying neuroinflammation in AD: the activity of the endocannabinoid system and the intestinal microbiota as neuroprotectors. This review highlights the importance of a broad multimodal approach to treat successfully the neuroinflammation underlying AD.
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants
Authors: Steiner Philip , Buchner Othmar , Andosch Ancuela , Wanner Gerhard , Neuner Gilbert , Lütz-Meindl Ursula
Published: November 2020

Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses coincide with distinct ultrastructural alterations. Three plants from different evolutionary levels and habitats were investigated: The freshwater alga Micrasterias denticulata, the aquatic plant Lemna sp., and the nival plant Ranunculus glacialis. Ultrastructural alterations during low temperature stress were determined by the employment of 2-D transmission electron microscopy and 3-D reconstructions from focused ion beam–scanning electron microscopic series. With decreasing temperatures, increasing numbers of organelle contacts and particularly the fusion of mitochondria to 3-dimensional networks were observed. We assume that the increase or at least maintenance of respiration during low temperature stress is likely to be based on these mitochondrial interconnections. Moreover, it is shown that autophagy and degeneration processes accompany freezing stress in Lemna and R. glacialis. This might be an essential mechanism to recycle damaged cytoplasmic constituents to maintain the cellular metabolism during freezing stress.
Nutrients, Genetic Factors, and Their Interaction in Non-Alcoholic Fatty Liver Disease and Cardiovascular Disease
Authors: Lombardi Rosa , Iuculano Federica , Pallini Giada , Fargion Silvia , Fracanzani Anna Ludovica
Published: November 2020

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in Western countries and expose patients to increased risk of hepatic and cardiovascular (CV) morbidity and mortality. Both environmental factors and genetic predisposition contribute to the risk. An inappropriate diet, rich in refined carbohydrates, especially fructose, and saturated fats, and poor in fibers, polyunsaturated fats, and vitamins is one of the main key factors, as well as the polymorphism of patatin-like phospholipase domain containing 3 (PNPLA3 gene) for NAFLD and the apolipoproteins and the peroxisome proliferator-activated receptor (PPAR) family for the cardiovascular damage. Beyond genetic influence, also epigenetics modifications are responsible for various clinical manifestations of both hepatic and CV disease. Interestingly, data are accumulating on the interplay between diet and genetic and epigenetic modifications, modulating pathogenetic pathways in NAFLD and CV disease. We report the main evidence from literature on the influence of both macro and micronutrients in NAFLD and CV damage and the role of genetics either alone or combined with diet in increasing the risk of developing both diseases. Understanding the interaction between metabolic alterations, genetics and diet are essential to treat the diseases and tailoring nutritional therapy to control NAFLD and CV risk.
You Talking to Me? Says the Enteric Nervous System (ENS) to the Microbe. How Intestinal Microbes Interact with the ENS
Authors: Giuffrè Mauro , Moretti Rita , Campisciano Giuseppina , da Silveira Alexandre Barcelos Morais , Monda Vincenzo Maria , Comar Manola , Di Bella Stefano , Antonello Roberta Maria , Luzzati Roberto , Crocè Lory Saveria
Published: November 2020

Mammalian organisms form intimate interfaces with commensal and pathogenic gut microorganisms. Increasing evidence suggests a close interaction between gut microorganisms and the enteric nervous system (ENS), as the first interface to the central nervous system. Each microorganism can exert a different effect on the ENS, including phenotypical neuronal changes or the induction of chemical transmitters that interact with ENS neurons. Some pathogenic bacteria take advantage of the ENS to create a more suitable environment for their growth or to promote the effects of their toxins. In addition, some commensal bacteria can affect the central nervous system (CNS) by locally interacting with the ENS. From the current knowledge emerges an interesting field that may shape future concepts on the pathogen–host synergic interaction. The aim of this narrative review is to report the current findings regarding the inter-relationships between bacteria, viruses, and parasites and the ENS.
The Differences between Gluten Sensitivity, Intestinal Biomarkers and Immune Biomarkers in Patients with First-Episode and Chronic Schizophrenia
Authors: Dzikowski Michał , Juchnowicz Dariusz , Dzikowska Izabela , Rog Joanna , Próchnicki Michał , Kozioł Małgorzata , Karakula-Juchnowicz Hanna
Published: November 2020

Schizophrenia is a heterogeneous disorder without a fully elucidated etiology and mechanisms. One likely explanation for the development of schizophrenia is low-grade inflammation, possibly caused by processes in the gastrointestinal tract related to gluten sensitivity. The aims of this study were to: (1) compare levels of markers of gluten sensitivity, inflammation and gut permeability, and (2) determine associations between gluten sensitivity, inflammation, and intestinal permeability in patients with first-episode/chronic (FS/CS) schizophrenia and healthy individuals (HC). The total sample comprised 162 individuals (52 FS; 50 CS, and 60 HC). The examination included clinical variables, nutritional assessment, and serum concentrations of: high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), soluble CD14 (sCD14), anti-Saccharomyces cerevisiae antibody (ASCA), antigliadin antibodies (AGA) IgA/IgG, antibodies against tissue transglutaminase 2 (anti-tTG) IgA, anti-deamidated gliadin peptides (anti-DGP) IgG. A significant difference between groups was found in sCD14, ASCA, hs-CRP, IL-6 and AGA IgA levels. AGA IgG/IgA levels were higher in the FS (11.54%; 30.77%) and CS (26%; 20%) groups compared to HC. The association between intestinal permeability and inflammation in the schizophrenic patients only was noted. The risk for developing schizophrenia was odds ratio (OR) = 4.35 (95% confidence interval (CI 1.23–15.39) for AGA IgA and 3.08 (95% CI 1.19–7.99) for positive AGA IgG. Inflammation and food hypersensitivity reactions initiated by increased intestinal permeability may contribute to the pathophysiology of schizophrenia. The immune response to gluten in FS differs from that found in CS.
Mental Health, Sense of Coherence, and Interpersonal Violence during the COVID-19 Pandemic Lockdown in Germany
Authors: Jung Stefanie , Kneer Jonas , Krüger Tillmann H. C.
Published: November 2020

Preliminary data indicates that the Coronavirus SARS-CoV-2 disease (COVID-19) pandemic may have a substantial impact on mental health and well-being. We assessed mental health in response to the lockdown in Germany between 1 April 2020 and 15 April 2020 using a cross-sectional online survey (n = 3545) with a mixed-methods approach. We found increased levels of psychosocial distress (Patient Health Questionnaire (PHQ) stress module), anxiety, depressive symptoms (PHQ-4), irritability, and a decrease in overall well-being (WHO-Five Well-Being Index (WHO-5)), sense of coherence (Short Form of the Sense of Coherence Scale (SOC-L9)), sexual contentment, and sleep quality. The four-week-prevalence of interpersonal violence was yet at 5% and included verbal, physical, and sexual violence. Participants reported finding comfort in family, friends, conversation, exercise, and activity. Findings are also in line with research showing that women seem to have more trouble coping with the pandemic and lockdown measures. Our observations demonstrate that the COVID-19 pandemic and related measures lead to a mental health burden even in a highly developed Western country and should, therefore, be taken seriously. The findings for interpersonal violence are alarming. Thus, we should sharpen our focus on the matter and activate and enhance supporting systems to help protect those affected.
Compression Therapy Is Not Contraindicated in Diabetic Patients with Venous or Mixed Leg Ulcer
Authors: Mosti Giovanni , Cavezzi Attilio , Bastiani Luca , Partsch Hugo
Published: November 2020

The aim of this study was to investigate if compression therapy (CT) can be safely applied in diabetic patients with Venous Leg Ulcers (VLU), even when a moderate arterial impairment (defined by an Ankle-Brachial Pressure Index 0.5–0.8) occurs as in mixed leg ulcers (MLU). Materials and methods: in one of our previous publications we compared the outcomes of two groups of patients with recalcitrant leg ulcers. Seventy-one patients were affected by mixed venous and arterial impairment and 109 by isolated venous disease. Both groups were treated by tailored inelastic CT (with compression pressure <40 mm Hg in patients with MLU and >60 mm Hg in patients with VLU) and ultrasound guided foam sclerotherapy (UGFS) of the superficial incompetent veins with the reflux directed to the ulcer bed. In the present sub analysis of the same patients we compared the healing time of 107 non-diabetic patients (NDP), 69 with VLU and 38 with MLU) with the healing time of 73 diabetic patients (DP), 40 with VLU and 33 with MLU. Results: Twenty-five patients were lost at follow up. The results refer to 155 patients who completed the treatment protocol. In the VLU group median healing time was 25 weeks for NDP and 28 weeks in DP (p = 0.09). In the MLU group median healing time was 27 weeks for NDP and 29 weeks for DP (p = −0.19). Conclusions: when providing leg ulcer treatment by means of tailored compression regimen and foam sclerotherapy for superficial venous refluxes, diabetes has only a minor or no effect on the healing time of recalcitrant VLU or MLU.
A One-Year Radiographic Healing Assessment after Endodontic Microsurgery Using Cone-Beam Computed Tomographic Scans
Authors: Kang Sumi , Ha Se-Won , Kim Ukseong , Kim Sunil , Kim Euiseong
Published: November 2020

This study aimed to evaluate one-year radiographic healing after endodontic microsurgery using CBCT with modified PENN 3D criteria and to compare the outcome with results evaluated using Molven’s criteria. A total of 107 teeth from 96 patients were evaluated one year after endodontic microsurgery by using CBCT scans with modified PENN 3D criteria and periapical radiographs with Molven’s criteria. Both preoperative and postoperative lesion volumes were calculated using ITK-SNAP (free software). Radiographic healing assessment using periapical radiographs and CBCT images, and preoperative and postoperative lesion volume measurements were performed independently by two examiners. The assessment using Molven’s criteria resulted in 75 complete healings, 18 incomplete healings, eight uncertain healings, and six unsatisfactory healings. Based on modified PENN 3D criteria, 64 teeth were categorized as complete healing, 29 teeth as limited healing, six teeth as uncertain healing, and eight teeth as unsatisfactory healing. With the one-year follow-up, CBCT scans showed a lower healing tendency than did periapical radiography. The volumes of apical radiolucency after the surgery were reduced by 77.7% on average at one-year follow up.
Communicating Intelligence Research
Authors: Wai Jonathan
Published: November 2020

Despite intelligence research being among the most replicable bodies of empirical findings—a Rosetta stone across the social sciences—the communication of intelligence research with non-intelligence researchers and the public remains a challenge, especially given ongoing public controversies throughout the history of the field. Hunt argued that “we have a communication problem.” This article is a call for intelligence researchers to consider communication at multiple levels—communication with other intelligence researchers, communication with non-intelligence researchers, and communication with the public, defined here as policymakers, practitioners, students, and general readers. It discusses ongoing tensions between academic freedom and social responsibility and provides suggestions for thinking about communication and effective research translation and implementation of intelligence research from the frameworks of science and policy research communication. It concludes with some recommendations for effective communication and stresses the importance of incentivizing more scholars to responsibly seek to educate and engage with multiple publics about the science of intelligence.
Multi-Omics Identifies Circulating miRNA and Protein Biomarkers for Facioscapulohumeral Dystrophy
Authors: Heier Christopher , Zhang Aiping , Nguyen Nhu , Tully Christopher , Panigrahi Aswini , Gordish-Dressman Heather , Pandey Sachchida , Guglieri Michela , Ryan Monique , Clemens Paula , Thangarajh Mathula , Webster Richard , Smith Edward , Connolly Anne , McDonald Craig , Karachunski Peter , Tulinius Mar , Harper Amy , Mah Jean , Fiorillo Alyson , Chen Yi-Wen ,   Cooperative International Neuromuscular Research Group (CINRG) Investigators
Published: November 2020

The development of therapeutics for muscle diseases such as facioscapulohumeral dystrophy (FSHD) is impeded by a lack of objective, minimally invasive biomarkers. Here we identify circulating miRNAs and proteins that are dysregulated in early-onset FSHD patients to develop blood-based molecular biomarkers. Plasma samples from clinically characterized individuals with early-onset FSHD provide a discovery group and are compared to healthy control volunteers. Low-density quantitative polymerase chain reaction (PCR)-based arrays identify 19 candidate miRNAs, while mass spectrometry proteomic analysis identifies 13 candidate proteins. Bioinformatic analysis of chromatin immunoprecipitation (ChIP)-seq data shows that the FSHD-dysregulated DUX4 transcription factor binds to regulatory regions of several candidate miRNAs. This panel of miRNAs also shows ChIP signatures consistent with regulation by additional transcription factors which are up-regulated in FSHD (FOS, EGR1, MYC, and YY1). Validation studies in a separate group of patients with FSHD show consistent up-regulation of miR-100, miR-103, miR-146b, miR-29b, miR-34a, miR-454, miR-505, and miR-576. An increase in the expression of S100A8 protein, an inflammatory regulatory factor and subunit of calprotectin, is validated by Enzyme-Linked Immunosorbent Assay (ELISA). Bioinformatic analyses of proteomics and miRNA data further support a model of calprotectin and toll-like receptor 4 (TLR4) pathway dysregulation in FSHD. Moving forward, this panel of miRNAs, along with S100A8 and calprotectin, merit further investigation as monitoring and pharmacodynamic biomarkers for FSHD.
The Road so Far in Colorectal Cancer Pharmacogenomics: Are We Closer to Individualised Treatment?
Authors: Simões Ana Rita , Fernández-Rozadilla Ceres , Maroñas Olalla , Carracedo Ángel
Published: November 2020

In recent decades, survival rates in colorectal cancer have improved greatly due to pharmacological treatment. However, many patients end up developing adverse drug reactions that can be severe or even life threatening, and that affect their quality of life. These remain a limitation, as they may force dose reduction or treatment discontinuation, diminishing treatment efficacy. From candidate gene approaches to genome-wide analysis, pharmacogenomic knowledge has advanced greatly, yet there is still huge and unexploited potential in the use of novel technologies such as next-generation sequencing strategies. This review summarises the road of colorectal cancer pharmacogenomics so far, presents considerations and directions to be taken for further works and discusses the path towards implementation into clinical practice.
The Contribution of Local Management to Biodiversity Conservation: An Analysis of Specific Cases in the Region of Madrid (Spain)
Authors: Molina-Holgado Pedro , López-Estébanez Nieves , Berrocal-Menárguez Ana-Belén , Allende-Álvarez Fernando , del Corro-Toro Miguel
Published: November 2020

In line with the Urban Agenda for the EU, this article highlights the importance of local actions in the conservation of biodiversity, both through specific activities and by increasing the availability of information. As such, the policies and projects related to the conservation of biodiversity have been analyzed here at different levels and, in particular, the initiatives undertaken in the Madrid Region, Spain. Consequently, two cases are presented that demonstrate the role that local administrations can play in improving the biodiversity database, and hence, in the effective protection of areas of significant environmental value. First, we will examine the effects that creating an environmental inventory of vegetation, flora and landscape has had in Torrelodones. Second, among the more recent environmental policies implemented in the municipality of Madrid are those that resulted in the environmental recovery of the urban section of the Manzanares River. Both these actions demonstrate how local authorities can contribute to the conservation of biodiversity at relatively low expense and in line with EU guidelines. Notably, this occurred despite the fact that competences in environmental matters in Spain are not municipal. In this context, the paper reflects on the untapped potential of the General Urban Planning Plans (PGOU) in deep knowledge and sustainable and responsible management of municipal environmental values.
Keeping α-Synuclein at Bay: A More Active Role of Molecular Chaperones in Preventing Mitochondrial Interactions and Transition to Pathological States?
Authors: Aspholm Emelie E. , Matečko-Burmann Irena , Burmann Björn M.
Published: November 2020

The property of molecular chaperones to dissolve protein aggregates of Parkinson-related α-synuclein has been known for some time. Recent findings point to an even more active role of molecular chaperones preventing the transformation of α-synuclein into pathological states subsequently leading to the formation of Lewy bodies, intracellular inclusions containing protein aggregates as well as broken organelles found in the brains of Parkinson’s patients. In parallel, a short motif around Tyr39 was identified as being crucial for the aggregation of α-synuclein. Interestingly, this region is also one of the main segments in contact with a diverse pool of molecular chaperones. Further, it could be shown that the inhibition of the chaperone:α-synuclein interaction leads to a binding of α-synuclein to mitochondria, which could also be shown to lead to mitochondrial membrane disruption as well as the possible proteolytic processing of α-synuclein by mitochondrial proteases. Here, we will review the current knowledge on the role of molecular chaperones in the regulation of physiological functions as well as the direct consequences of impairing these interactions—i.e., leading to enhanced mitochondrial interaction and consequential mitochondrial breakage, which might mark the initial stages of the structural transition of α-synuclein towards its pathological states.
Anti-Inflammatory Effects of Fermented Lotus Root and Linoleic Acid in Lipopolysaccharide-Induced RAW 264.7 Cells
Authors: Kim Sung Min , Park Eun-Jung , Kim Jong-Yeon , Choi Jihee , Lee Hae-Jeung
Published: November 2020

Inflammation is a protective response of the innate immune system. However, aberrant inflammatory responses lead to various diseases. Lotus root, the edible rhizome of Nelumbo nucifera, is a popular traditional herbal medicine in East Asia. In a previous study, we reported that fermented lotus root (FLR) alleviated ethanol/HCl-induced gastric ulcers in rats by modulating inflammation-related genes. However, the mechanisms underlying the anti-inflammatory effects of FLR and its major constituent, linoleic acid (LA), are still largely unknown. In this study, we investigated the anti-inflammatory effects of FLR and LA on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 murine macrophages. We found that FLR inhibited LPS-induced expression of inflammatory mediators through down-regulation of NF-κB activity. Similarly, LA also attenuated LPS-induced inflammatory responses and reduced LPS-induced phosphorylation of proteins associated with NF-κB signaling, such as ERK, JNK, and p38. Overall, our results suggested that FLR and LA may effectively ameliorate inflammatory diseases.
A Study on the Utilization of Coal Fly Ash Derived Grog in Clay Ceramics
Authors: Choo Thye , Mohd Salleh Mohamad , Kok Kuan , Matori Khamirul , Abdul Rashid Suraya
Published: November 2020

Grog is an additive material that plays important roles in ceramic making. It improves the fabrication process of green bodies as well as the physical properties of fired bodies. Few low-cost materials and wastes have found their application as grog in recent years, thus encouraging the replacement of commercial grogs with cost-saving materials. Coal fly ash, a combustion waste produced by coal-fired power plant, has the potential to be converted into grog owing to its small particle sizes and high content of silica and alumina. In this study, grog was derived from coal fly ash and mixed with kaolin clay to produce ceramics. Effects of the grog addition on the resultant ceramics were investigated. It was found that, to a certain extent, the grog addition reduced the firing shrinkage and increased the total porosity of the ceramics. The dimensional stability of the ceramics at a firing temperature of 1200 °C was also not noticeably affected by the grog. However, the grog addition in general had negative effects on the biaxial flexural strength and refractoriness of the ceramics.
Pillared Graphene Structures Supported by Vertically Aligned Carbon Nanotubes as the Potential Recognition Element for DNA Biosensors
Authors: Shunaev Vladislav V. , Glukhova Olga E.
Published: November 2020

The development of electrochemical biosensors is an important challenge in modern biomedicine since they allow detecting femto- and pico-molar concentrations of molecules. During this study, pillared graphene structures supported by vertically aligned carbon nanotubes (VACNT-graphene) are examined as the potential recognition element of DNA biosensors. Using mathematical modeling methods, the atomic supercells of different (VACNT-graphene) configurations and the energy profiles of its growth are found. Regarding the VACNT(12,6)-graphene doped with DNA nitrogenous bases, calculated band structure and conductivity parameters are used. The obtained results show the presence of adenine, cytosine, thymine, and guanine on the surface of VACNT(12,6)-graphene significantly changes its conductivity so the considered object could be the prospective element for DNA biosensing.
Prediction of Mechanical Properties by Artificial Neural Networks to Characterize the Plastic Behavior of Aluminum Alloys
Authors: Merayo David , Rodríguez-Prieto Alvaro , Camacho Ana María
Published: November 2020

In metal forming, the plastic behavior of metallic alloys is directly related to their formability, and it has been traditionally characterized by simplified models of the flow curves, especially in the analysis by finite element simulation and analytical methods. Tools based on artificial neural networks have shown high potential for predicting the behavior and properties of industrial components. Aluminum alloys are among the most broadly used materials in challenging industries such as aerospace, automotive, or food packaging. In this study, a computer-aided tool is developed to predict two of the most useful mechanical properties of metallic materials to characterize the plastic behavior, yield strength and ultimate tensile strength. These prognostics are based on the alloy chemical composition, tempers, and Brinell hardness. In this study, a material database is employed to train an artificial neural network that is able to make predictions with a confidence greater than 95%. It is also shown that this methodology achieves a performance similar to that of empirical equations developed expressly for a specific material, but it provides greater generality since it can approximate the properties of any aluminum alloy. The methodology is based on the usage of artificial neural networks supported by a big data collection about the properties of thousands of commercial materials. Thus, the input data go above 2000 entries. When the relevant information has been collected and organized, an artificial neural network is defined, and after the training, the artificial intelligence is able to make predictions about the material properties with an average confidence greater than 95%.
Fire-Temperature Influence on Portland and Calcium Sulfoaluminate Blend Composites
Authors: Sodol Konrad A. , Kaczmarek Łukasz , Szer Jacek
Published: November 2020

This paper presents the research data of the fire-temperature influence on Portland CEM I (OPC) and calcium sulfoaluminate (CSA) types of cement blend composites as cooling materials dedicated for infill and covers in fire systems. The data present the material responses for four types at high-temperature elevation times (0, 15, 30, 60 min), such as core heat curves, differences in specimens color, flexural and compressive strength parameters. Materials were tested using the DSC method to collect information about enthalpies. The differences between cement blend composites were compared with commonly used cooling materials such as gypsum blends. It is shown that modifications to Portland cement composites by calcium sulfoaluminate cement have a significant influence on the cooling performance during high-temperature, even for 60 min of exposure. The temperature increase rates in the material core were slower in composites with regards to additionally containing calcium sulfoaluminate in 100–150 °C range. After 60 min of high-temperature elevation, the highest flexural and compressive strength was 75% OPC/25% CSA cement composition. The influence on cooling properties was not related to strength properties. The presented solution may have a significant influence as a passive extinguisher solution of future fire resistance systems in civil engineering.
Abrasive Sensitivity of Engineering Polymers and a Bio-Composite under Different Abrasive Conditions
Authors: Muhandes Hasan , Kalácska Ádám , Székely László , Keresztes Róbert , Kalácska Gábor
Published: November 2020

Two different test systems were designed to evaluate the tribological behavior of five engineering plastics (Polyamide—PA grades and Ultra High Molecular Weight Polyethylene—UHMW-PE) and a fully degradable bio-composite (Polylactic Acid—PLA/hemp fibers) targeted to agricultural machinery abrasive conditions. Pin-on-plate tests were performed with different loads, sliding velocity and abrasive particles. The material response was further investigated in a slurry containing abrasive test system with different sliding velocities and distances, abrasive media compositions and impact angles. The abrasive wear, the change of the 3D surface roughness parameters, the friction force and contact temperature evolution were also analyzed as a function of the materials’ mechanical properties (H,E,σy,σc,εB,σF,σM) and the dimensionless numbers derived from them. Using the IBM SPSS 25 software, multiple linear regression models were used to statistically evaluate the measured data and to examine the sensitivity of the material properties and test system characteristics on the tribological behavior. For both test setups, the system and material characteristics influencing the dependent variables (wear, friction, heat generation) and the dimensionless numbers formed from the material properties were ranked using standardized regression coefficients derived from the regression models. The abrasion sensitivity of the tested materials were evaluated taking into account a wide range of influencing parameters.
MEA Preparation for Direct Formate/Formic Acid Fuel Cell—Comparison of Palladium Black and Palladium Supported on Activated Carbon Performance on Power Generation in Passive Fuel Cell
Authors: Nogalska Adrianna , Navarro Andreu Bonet , Garcia-Valls Ricard
Published: November 2020

Membrane electrode assemblies (MEAs) with palladium catalysts were successfully prepared by using a home-made manual pressing system with Nafion glue application that contributed to a decrease of additional energy consumption. The catalyst coated membranes were prepared with supported palladium on activated carbon (PdC) and unsupported palladium black (PdB) for comparison. The performance of passive, air breathing, functioning under ambient conditions and with low concentration (1 M) formate/formic acid fuel cell was evaluated. Based on polarization curves, the best result was obtained with carbon supported catalyst and HCOOK fuel, achieving 21.01 mW/mgPd. Still, constant current discharge with PdC showed an energy generation efficiency of 14% with HCOOH over 3% with HCOOK caused by lower potassium ion conductivity and its permeability through the proton exchange membrane. The faradic efficiency of conversion in the cell is equal to the overall energy efficiency and makes the cell self-sufficient.
Influence of Sodium Phosphate Salts with Different Chain Length on the Flotation Behavior of Magnesite and Dolomite
Authors: Bai Junzhi , Wang Jizhen , Yin Wanzhong , Chen Xiangxiang
Published: November 2020

This paper analyzes the influence of sodium phosphate salts with different chain lengths as depressants on the flotation behavior of magnesite and dolomite through single mineral flotation test, contact angle test, and theoretical analysis. Flotation tests show that depressants should be added for the flotation separation of magnesite and dolomite. The inhibition of sodium phosphate salts on dolomite is significantly stronger than magnesite, and the flotation difference of minerals is affected by the chain length of phosphate depressants. The order of flotation separation enhancement of different sodium phosphate depressants is sodium hexametaphosphate ≈ sodium tetrapolyphosphate > sodium tripolyphosphate > sodium pyrophosphate. This result could also be supported by the contact angle measurement.
Flavonoid Preparations from Taraxacum officinale L. Fruits—A Phytochemical, Antioxidant and Hemostasis Studies
Authors: Lis Bernadetta , Jedrejek Dariusz , Rywaniak Joanna , Soluch Agata , Stochmal Anna , Olas Beata
Published: November 2020

Dandelion (Taraxacum officinale L.) roots, leaves, and flowers have a long history of use in traditional medicine. Compared to the above organs, dandelion fruits are the least known and used. Hence, the present paper was aimed at the phytochemical analysis of T. officinale fruit extract and estimating its antiradical, antiplatelet, and antioxidant properties related to hemostasis. Methanolic extract of fruits (E1), enriched with polyphenols (188 mg gallic acid equivalents (GAE)/g), was successfully separated into cinnamic acids (E2; 448 mg GAE/g) and flavonoids (E3; 377 mg GAE/g) extracts. Flavonoid extract was further divided into four fractions characterized by individual content: A (luteolin fraction; 880 mg GAE/g), B (philonotisflavone fraction; 516 mg GAE/g), C (flavonolignans fraction; 384 mg GAE/g), and D (flavone aglycones fraction; 632 mg GAE/g). High DPPH radical scavenging activity was evaluated for fractions A and B (A > B > Trolox), medium for extracts (Trolox > E3 > E2 > E1), and low for fractions C and D. No simple correlation between polyphenol content and antiradical activity was observed, indicating a significant influence of qualitative factor, including higher anti-oxidative effect of flavonoids with B-ring catechol system compared to hydroxycinnamic acids. No cytotoxic effect on platelets was observed for any dandelion preparation tested. In experiments on plasma and platelets, using several different parameters (lipid peroxidation, protein carbonylation, oxidation of thiols, and platelet adhesion), the highest antioxidant and antiplatelet potential was demonstrated by three fruit preparations–hydroxycinnamic acids extract (E2), flavonoid extract (E3), and luteolin fraction (A). The results of this paper provide new information on dandelion metabolites, as well as their biological potential and possible use concerning cardiovascular diseases.
Critical Review of Existing MHC I Immunopeptidome Isolation Methods
Authors: Kuznetsov Alexandr , Voronina Alice , Govorun Vadim , Arapidi Georgij
Published: November 2020

Major histocompatibility complex class I (MHC I) plays a crucial role in the development of adaptive immune response in vertebrates. MHC molecules are cell surface protein complexes loaded with short peptides and recognized by the T-cell receptors (TCR). Peptides associated with MHC are named immunopeptidome. The MHC I immunopeptidome is produced by the proteasome degradation of intracellular proteins. The knowledge of the immunopeptidome repertoire facilitates the creation of personalized antitumor or antiviral vaccines. A huge number of publications on the immunopeptidome diversity of different human and mouse biological samples—plasma, peripheral blood mononuclear cells (PBMCs), and solid tissues, including tumors—appeared in the scientific journals in the last decade. Significant immunopeptidome identification efficiency was achieved by advances in technology: the immunoprecipitation of MHC and mass spectrometry-based approaches. Researchers optimized common strategies to isolate MHC-associated peptides for individual tasks. They published many protocols with differences in the amount and type of biological sample, amount of antibodies, type and amount of insoluble support, methods of post-fractionation and purification, and approaches to LC-MS/MS identification of immunopeptidome. These parameters have a large impact on the final repertoire of isolated immunopeptidome. In this review, we summarize and compare immunopeptidome isolation techniques with an emphasis on the results obtained.
Fluorinated Organic Paramagnetic Building Blocks for Cross-Coupling Reactions
Authors: Politanskaya Larisa V. , Fedyushin Pavel A. , Rybalova Tatyana V. , Bogomyakov Artem S. , Asanbaeva Nargiz B. , Tretyakov Evgeny V.
Published: November 2020

New stable polyfluorinated nitroxide radicals for use in cross-coupling reactions, namely, N-tert-butyl-N-oxyamino-2,3,5,6-tetrafluoro-4-iodobenzene and N-tert-butyl-N-oxyamino-2,3,5,6-tetrafluoro-4-ethynylbenzene, were prepared from perfluoroiodobenzene. The reaction of the polyfluoro derivative with tert-butylamine under autoclaving conditions leading to the formation of N-tert-butyl-2,3,5,6-tetrafluoro-4-iodoaniline proved to be the key stage of the whole process. The fluorinated tert-butyl iodophenyl nitroxide was found to form in a solid state via N–O···I halogen bonds, a one-dimensional assembly of the radicals. The acceptor role of the nitroxide group in the halogen bonding changes to a donor role when the nitroxide reacts with Cu(hfac)2. In the last case, zero-dimensional assembly prevails, giving a three-spin complex with axial coordinated nitroxide groups and, as a consequence, causing ferromagnetic intramolecular exchange interactions between Cu(II) and radical spins.
Clinical Symptoms of Arboviruses in Mexico
Authors: Ananth Sushmitha , Shrestha Nistha , Treviño C. Jesús A. , Nguyen Uyen-sa , Haque Ubydul , Angulo-Molina Aracely , Lopez-Lemus Uriel A. , Lubinda Jailos , Sharif Rashed Md. , Zaki Rafdzah Ahmad , Sánchez Casas Rosa María , Cervantes Diana , Nandy Rajesh
Published: November 2020

Arboviruses such as Chikungunya (CHIKV), Dengue (DENV), and Zika virus (ZIKV) have emerged as a significant public health concern in Mexico. The existing literature lacks evidence regarding the dispersion of arboviruses, thereby limiting public health policy’s ability to integrate the diagnosis, management, and prevention. This study seeks to reveal the clinical symptoms of CHIK, DENV, and ZIKV by age group, region, sex, and time across Mexico. The confirmed cases of CHIKV, DENV, and ZIKV were compiled from January 2012 to March 2020. Demographic characteristics analyzed significant clinical symptoms of confirmed cases. Multinomial logistic regression was used to assess the association between clinical symptoms and geographical regions. Females and individuals aged 15 and older had higher rates of reported significant symptoms across all three arboviruses. DENV showed a temporal variation of symptoms by regions 3 and 5, whereas ZIKV presented temporal variables in regions 2 and 4. This study revealed unique and overlapping symptoms between CHIKV, DENV, and ZIKV. However, the differentiation of CHIKV, DENV, and ZIKV is difficult, and diagnostic facilities are not available in rural areas. There is a need for adequately trained healthcare staff alongside well-equipped lab facilities, including hematological tests and imaging facilities.
Evaluation of Five International HBV Treatment Guidelines: Recommendation for Resource-Limited Developing Countries Based on the National Study in Nepal
Authors: Khadka Sundar , Pandit Roshan , Dhital Subhash , Baniya Jagat Bahadur , Tiwari Surendra , Shrestha Bimal , Pandit Sanjeet , Sato Fumitaka , Fujita Mitsugu , Sharma Mukunda , Tsunoda Ikuo , Mishra Shravan Kumar
Published: November 2020

Hepatitis B virus (HBV) infects the liver, causing cirrhosis and cancer. In developed countries, five international guidelines have been used to make a decision for the management of patients with chronic HBV infection. In this review, since the guidelines were established by clinical and epidemiological data of developed countries, we aimed to evaluate whether (1) HBV patient profiles of developing countries are similar to developed countries, and (2) which guideline can be applicable to resource-limited developing countries. First, as an example of the most recent data of HBV infections among developing countries, we evaluated the national HBV viral load study in Nepal, which were compared with the data from other developing countries. In Nepal, the highest number of patients had viral loads of 20–2000 IU/mL (36.7%) and belonged to the age group of 21–30 years; HBV epidemiology in Nepal, based on the viral loads, gender, and age groups was similar to those of not only other developing countries but also developed countries. Next, we reviewed five international HBV treatment guidelines of the World Health Organization (WHO), American Association for the Study of Liver Diseases (AASLD), National Institute for Health and Care Excellence (NICE), European Association for the Study of the Liver (EASL), and Asian Pacific Association for the Study of the Liver (APASL). All guidelines require the viral load and alanine aminotransferase (ALT) levels for decision making. Although four guidelines recommend elastography to assess liver cirrhosis, the WHO guideline alternatively recommends using the aspartate aminotransferase (AST)-to-platelet ratio index (APRI), which is inexpensive and conducted routinely in most hospitals. Therefore, in resource-limited developing countries like Nepal, we recommend the WHO guideline for HBV treatment based on the viral load, ALT, and APRI information.
Synthesis and Biological Evaluation of Carvacrol-Based Derivatives as Dual Inhibitors of H. pylori Strains and AGS Cell Proliferation
Authors: Sisto Francesca , Carradori Simone , Guglielmi Paolo , Traversi Carmen Beatrice , Spano Mattia , Sobolev Anatoly P. , Secci Daniela , Di Marcantonio Maria Carmela , Haloci Entela , Grande Rossella , Mincione Gabriella
Published: November 2020

This study reports on the synthesis, structural assessment, microbiological screening against several strains of H. pylori and antiproliferative activity against human gastric adenocarcinoma (AGS) cells of a large series of carvacrol-based compounds. Structural analyses consisted of elemental analysis, 1H/13C/19F NMR spectra and crystallographic studies. The structure-activity relationships evidenced that among ether derivatives the substitution with specific electron-withdrawing groups (CF3 and NO2) especially in the para position of the benzyl ring led to an improvement of the antimicrobial activity, whereas electron-donating groups on the benzyl ring and ethereal alkyl chains were not tolerated with respect to the parent compound (MIC/MBC = 64/64 µg/mL). Ester derivatives (coumarin-carvacrol hybrids) displayed a slight enhancement of the inhibitory activity up to MIC values of 8–16 µg/mL. The most interesting compounds exhibiting the lowest MIC/MBC activity against H. pylori (among others, compounds 16 and 39 endowed with MIC/MBC values ranging between 2/2 to 32/32 µg/mL against all the evaluated strains) were also assayed for their ability to reduce AGS cell growth with respect to 5-Fluorouracil. Some derivatives can be regarded as new lead compounds able to reduce H. pylori growth and to counteract the proliferation of AGS cells, both contributing to the occurrence of gastric cancer.
Optimising PLGA-PEG Nanoparticle Size and Distribution for Enhanced Drug Targeting to the Inflamed Intestinal Barrier
Authors: Mohan Lauren J. , McDonald Lauren , Daly Jacqueline S. , Ramtoola Zebunnissa
Published: November 2020

Oral nanomedicines are being investigated as an innovative strategy for targeted drug delivery to treat inflammatory bowel diseases. Preclinical studies have shown that nanoparticles (NPs) can preferentially penetrate inflamed intestinal tissues, allowing for targeted drug delivery. NP size is a critical factor affecting their interaction with the inflamed intestinal barrier and this remains poorly defined. In this study we aimed to assess the impact of NP particle size (PS) and polydispersity (PDI) on cell interaction and uptake in an inflamed epithelial cell model. Using 10, 55 and 100 mg/mL poly(lactic-co-glycolic acid)-polyethylene glycol (PLGA-PEG), NPs of 131, 312 and 630 nm PS, respectively, were formulated by solvent dispersion. NP recovery was optimised by differential centrifugation to yield NPs of decreased and unimodal size distribution. NP-cell interaction was assessed in healthy and inflamed caco-2 cell monolayers. Results show that NP interaction with caco-2 cells increased with increasing PS and PDI and was significantly enhanced in inflamed cells. Trypan blue quenching revealed that a significant proportion of multimodal NPs were primarily membrane bound, while monomodal NPs were internalized within cells. These results are interesting as the PS and PDI of NPs can be optimised to allow targeting of therapeutic agents to the epithelial membrane and/or intracellular targets in the inflamed intestinal epithelium.
Mechanisms of Antidiabetic Activity of Methanolic Extract of Punica granatum Leaves in Nicotinamide/Streptozotocin-Induced Type 2 Diabetes in Rats
Authors: Pottathil Shinu , Nain Parminder , Morsy Mohamed A. , Kaur Jaspreet , Al-Dhubiab Bandar E. , Jaiswal Sandhya , Nair Anroop B.
Published: November 2020

The current study aimed to establish the mechanisms of antidiabetic activity of methanolic extract of Punica granatum leaves (MEPGL) in nicotinamide/streptozotocin-induced type 2 diabetes in rats. Phytochemical screening, HPLC analysis, and acute toxicity study of MEPGL were carried out. Various concentrations of MEPGL (100, 200, 400, and 600 mg/kg) were administered orally to diabetic rats for 45 days on a daily basis. The antidiabetic effect of MEPGL was examined by measuring blood glucose, plasma insulin, and glycated hemoglobin (HbA1c) levels, as well as with an oral glucose tolerance test. The antioxidant effect of MEPGL was determined by analyzing hepatic and renal antioxidant markers, namely superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and lipid peroxidation. The other biochemical markers alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), urea, and creatinine, as well as total cholesterol, triglycerides, and high-density lipoprotein (HDL) were also studied. Type 2 diabetes significantly altered these parameters, while oral administration of the MEPGL significantly ameliorated them. Moreover, the pancreatic histopathological changes were attenuated with MEPGL treatment. In a nutshell, oral MEPGL administration in diabetic rats showed antidiabetic activity due to its antioxidant activity, most probably due to the gallic acid, ellagic acid, and apigenin found in MEPGL.