bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Aerobic Exercise on Oxidative Stress and Inflammatory Response During Particulate Matter Exposure in Mouse Lungs
Authors: So Byunghun , Park Jinhan , Jang Junho , Lim Wonchung , Imdad Saba , Kang Chounghun
Published: February 2022

Regular exercise provides several health benefits that can improve the cardiovascular and musculoskeletal systems, but clear evidence on the effect of exercise-induced hyperventilation in particulate matter (PM) exposure is still lacking. This study aimed to investigate the effects of exercise in PM exposure on reactive oxygen species (ROS) generation, inflammatory response, and mitochondrial integrity in human lung epithelial cells (A549), as well as in mouse lung tissue. In in vitro experiments, PM treatment was shown to significantly increased ROS production, and reduced cell viability and mitochondrial function in A549 cells. The mice were divided into four groups for an in vivo exercise experiment: control (CON), PM inhalation (PI), PM inhalation during exercise (PIE), and exercise (EX) groups. The PI and PIE groups were exposed to 100 μg/m3 of PM for 1 h per day for a week. The PIE and EX groups performed treadmill exercises every day for 1 h at 20 m/min for a week. The levels of pro-inflammatory markers (IL-6 and TNF-α) were significantly higher in the PI group than in the CON group (P < 0.001 and P < 0.01, respectively). The carbonyl protein level was decreased in EX vs. PI (P < 0.001). Mitochondrial fission (Drp1) content was significantly decreased in the EX vs. CON group (P < 0.01), but anti-mitochondrial fission (P-Drp1 Ser637) was increased in the EX vs. PI group (P < 0.05). Mitochondrial autophagy (mitophagy), which is an assessment of mitochondrial integrity, was markedly increased in PI vs. CON (P < 0.001), but the level was reversed in PIE (P < 0.05). Lung fibrosis was increased in PI vs. CON group (P < 0.001), however, the cells were rescued in the PIE (P < 0.001). The number of apoptotic cells was remarkably increased in the PI vs. CON group (P < 0.001), whereas the level was decreased in the PIE (P < 0.001). Taken together, these results showed that short-term exposure to PM triggers oxidative stress, pro-inflammatory responses, and apoptosis in the lungs, but the PM-induced adverse effects on the lung tissue are not exacerbated by exercise-induced PM hyperventilation but rather has a protective effect.
Maternal and Fetal-Placental Effects of Etanercept Treatment During Rats’ Pregnancy
Authors: Araujo Gabriel Gomes , dos Passos Junior Rinaldo Rodrigues , Lunardi Rosaline Rocha , Volpato Gustavo Tadeu , Soares Thaigra Sousa , Giachini Fernanda Regina , Lima Victor Vitorino
Published: February 2022

Etanercept is a tumor necrosis factor alpha (TNF-α) inhibitor chronically used to treat autoimmune diseases. However, the use of etanercept during pregnancy still needs to be further investigated. The aim of this study is to evaluate the etanercept treatment during pregnancy, analyzing maternal reproductive performance, fetal outcomes, and placental repercussions. Wistar rats (200–250 g) were mated and randomly distributed into two experimental groups: control and etanercept (n = 10 animals/group). Treatments with etanercept (0.8 mg/kg, s.c.), or saline (control group) were carried out on days 0, 6, 12, and 18 of gestation. On the morning of the 21st day of pregnancy, rats were euthanized in a CO2 chamber and submitted to laparotomy to remove the fetuses, placentas, ovaries, and maternal organs. There were no differences between groups in the following parameters: water and food consumption; placental efficiency; reproductive parameters, including number of corpora lutea and implants, reabsorption, and pre- and post-implantation losses. However, etanercept treatment increased liver weight, reduced fetal and placental weight, decreased the placental junction zone, reduced the percentage of normal fetuses, and increased visceral or skeletal fetal abnormalities. Therefore, etanercept resulted in damages more related to fetus and placenta. However, more studies with different doses are required to better predict possible injuries elicited using etanercept during pregnancy.
The Roles of Transient Receptor Potential Ion Channels in Pathologies of Glaucoma
Authors: Ma Lin , Liu Xin , Liu Qing , Jin Sen , Chang Heng , Liu Haixia
Published: February 2022

Transient receptor ion potential (TRP) channels are a cluster of non-selective cation channels present on cell membranes. They are important mediators of sensory signals to regulate cellular functions and signaling pathways. Alterations and dysfunction of these channels could disrupt physiological processes, thus leading to a broad array of disorders, such as cardiovascular, renal and nervous system diseases. These effects position them as potential targets for drug design and treatment. Because TRP channels can mediate processes such as mechanical conduction, osmotic pressure, and oxidative stress, they have been studied in the context of glaucoma. Glaucoma is an irreversible blinding eye disease caused by an intermittent or sustained increase in intraocular pressure (IOP), which results in the apoptosis of retinal ganglion cells (RGCs), optic nerve atrophy and eventually visual field defects. An increasing number of studies have documented that various TRP subfamilies are abundantly expressed in ocular structures, including the cornea, lens, ciliary body (CB), trabecular meshwork (TM) and retina. In alignment with these findings, there is also mounting evidence supporting the potential role of the TRP family in glaucoma progression. Therefore, it is of great interest and clinical significance to gain an increased understanding of these channels, which in turn could shed more light on the identification of new therapeutic targets for glaucoma. Moreover, this role is not understood completely to date, and whether the activation of TRP channels contributes to glaucoma, or instead aggravates progression, needs to be explored. In this manuscript, we aim to provide a comprehensive overview of recent research on TRP channels in glaucoma and to suggest novel targets for future therapeutic interventions in glaucoma.
Comparison of Different Recovery Strategies After High-Intensity Functional Training: A Crossover Randomized Controlled Trial
Authors: Martínez-Gómez Rafael , Valenzuela Pedro L. , Lucia Alejandro , Barranco-Gil David
Published: February 2022

We aimed to determine whether voluntary exercise or surface neuromuscular electrical stimulation (NMES) could enhance recovery after a high-intensity functional training (HIFT) session compared with total rest. The study followed a crossover design. Fifteen male recreational CrossFit athletes (29 ± 8 years) performed a HIFT session and were randomized to recover for 15 min with either low-intensity leg pedaling (“Exercise”), NMES to the lower limbs (“NMES”), or total rest (“Control”). Perceptual [rating of perceived exertion (RPE) and delayed-onset muscle soreness (DOMS) of the lower-limb muscles], physiological (heart rate, blood lactate and muscle oxygen saturation) and performance (jump ability) indicators of recovery were assessed at baseline and at different time points during recovery up to 24 h post-exercise. A significant interaction effect was found for RPE (p = 0.035), and although post hoc analyses revealed no significant differences across conditions, there was a quasi-significant (p = 0.061) trend toward a lower RPE with NMES compared with Control immediately after the 15-min recovery. No significant interaction effect was found for the remainder of outcomes (all p > 0.05). Except for a trend toward an improved perceived recovery with NMES compared with Control, low-intensity exercise, NMES, and total rest seem to promote a comparable recovery after a HIFT session.
Differential Influence of Physical Activity on Cardiopulmonary Performance and Stroke Volume Assessed at Cardiopulmonary Exercise Test in Pectus Excavatum: A Pilot Study
Authors: Casatori Lorenzo , Pellegrino Alessio , Messineo Antonio , Ghionzoli Marco , Facchini Flavio , Modesti Alessandra , Modesti Pietro Amedeo
Published: February 2022

Background

Exercise training increases muscle VO2 by increasing O2 transport and O2 uptake while cardiac output increase might be limited by the conformation of the chest in subjects with pectus excavatum (PE).

Aims

The aim of the present study was to investigate the influence of physical activity (PA) on functional parameters of cardiopulmonary performance and stroke volume obtained at Cardiopulmonary Exercise Test (CPET) in PE.

Methods and Procedures

A cohort of adolescents (15 with PE and 15 age- and sex-matched healthy controls, HC) underwent Cardiopulmonary Exercise Test (CPET) and administration of the International Physical Activity Questionnaire – Short Form (IPAQ-SF) with estimation of weekly PA (METs h–1⋅week–1). Determinants of CPET parameters were investigated with multivariable linear regression analysis.

Results

As expected, when compared to HC, PE had lower VO2 max (37.2 ± 6.6 vs. 45.4 ± 6.4 mL⋅kg–1⋅min–1, p < 0.05), and VO2/HR max (O2 pulse, 12.1 ± 2.4 vs. 16.2 ± 3.6 mL⋅min–1⋅bpm–1, p < 0.05). Importantly, physical activity level was a predictor of VO2 max (adjusted for sex, body mass index, FEV1%, and presence of PE, β = 0.085; 95% Cl 0.010 to 0.160, p = 0.029) whereas O2 pulse was independent from PA level (β = 0.035; 95% Cl −0.004 to 0.074).

Conclusion

Physical activity is a determinant of VO2 max (cardiopulmonary performance), whereas it appears not to affect O2 pulse (a measure of stroke volume at peak exercise) related to constrained diastolic filling in PE.
Determinants of Peroxisome Membrane Dynamics
Authors: Carmichael Ruth E. , Schrader Michael
Published: February 2022

Organelles within the cell are highly dynamic entities, requiring dramatic morphological changes to support their function and maintenance. As a result, organelle membranes are also highly dynamic, adapting to a range of topologies as the organelle changes shape. In particular, peroxisomes—small, ubiquitous organelles involved in lipid metabolism and reactive oxygen species homeostasis—display a striking plasticity, for example, during the growth and division process by which they proliferate. During this process, the membrane of an existing peroxisome elongates to form a tubule, which then constricts and ultimately undergoes scission to generate new peroxisomes. Dysfunction of this plasticity leads to diseases with developmental and neurological phenotypes, highlighting the importance of peroxisome dynamics for healthy cell function. What controls the dynamics of peroxisomal membranes, and how this influences the dynamics of the peroxisomes themselves, is just beginning to be understood. In this review, we consider how the composition, biophysical properties, and protein-lipid interactions of peroxisomal membranes impacts on their dynamics, and in turn on the biogenesis and function of peroxisomes. In particular, we focus on the effect of the peroxin PEX11 on the peroxisome membrane, and its function as a major regulator of growth and division. Understanding the roles and regulation of peroxisomal membrane dynamics necessitates a multidisciplinary approach, encompassing knowledge across a range of model species and a number of fields including lipid biochemistry, biophysics and computational biology. Here, we present an integrated overview of our current understanding of the determinants of peroxisome membrane dynamics, and reflect on the outstanding questions still remaining to be solved.
Muscle Adaptations to Heavy-Load and Blood Flow Restriction Resistance Training Methods
Authors: May Anthony K. , Russell Aaron P. , Della Gatta Paul A. , Warmington Stuart A.
Published: February 2022

Resistance-based blood flow restriction training (BFRT) improves skeletal muscle strength and size. Unlike heavy-load resistance training (HLRT), there is debate as to whether strength adaptations following BFRT interventions can be primarily attributed to concurrent muscle hypertrophy, as the magnitude of hypertrophy is often minor. The present study aimed to investigate the effect of 7 weeks of BFRT and HLRT on muscle strength and hypertrophy. The expression of protein growth markers from muscle biopsy samples was also measured. Male participants were allocated to moderately heavy-load training (HL; n = 9), low-load BFRT (LL + BFR; n = 8), or a control (CON; n = 9) group to control for the effect of time. HL and LL + BFR completed 21 training sessions (3 d.week−1) comprising bilateral knee extension and knee flexion exercises (HL = 70% one-repetition maximum (1-RM), LL + BFR = 20% 1-RM + blood flow restriction). Bilateral knee extension and flexion 1-RM strength were assessed, and leg muscle CSA was measured via peripheral quantitative computed tomography. Protein growth markers were measured in vastus lateralis biopsy samples taken pre- and post the first and last training sessions. Biopsy samples were also taken from CON at the same time intervals as HL and LL + BFR. Knee extension 1-RM strength increased in HL (19%) and LL + BFR (19%) but not CON (2%; p < 0.05). Knee flexion 1-RM strength increased similarly between all groups, as did muscle CSA (50% femur length; HL = 2.2%, LL + BFR = 3.0%, CON = 2.1%; TIME main effects). 4E-BP1 (Thr37/46) phosphorylation was lower in HL and LL + BFR immediately post-exercise compared with CON in both sessions (p < 0.05). Expression of other growth markers was similar between groups (p > 0.05). Overall, BFRT and HLRT improved muscle strength and size similarly, with comparable changes in intramuscular protein growth marker expression, both acutely and chronically, suggesting the activation of similar anabolic pathways. However, the low magnitude of muscle hypertrophy was not significantly different to the non-training control suggesting that strength adaptation following 7 weeks of BFRT is not driven by hypertrophy, but rather neurological adaptation.
Differentiation of Cold Tolerance in an Artificial Population of a Mangrove Species, Kandelia obovata, Is Associated With Geographic Origins
Authors: Lu Wen-Xun , Zhang Bing-Huang , Zhang Yuan-Ye , Yang Sheng-Chang
Published: February 2022

Temperature is one of the climatic factors that shape the geographic distribution of plant populations. Mangroves are temperature-sensitive plants, and their distributions are severely limited by low temperatures. It is unknown, however, to what extent temperature contributes to their population differentiation and evolution. Kandelia obovata (Rhizophoraceae) is a mangrove species with high cold tolerance in the Northern Hemisphere. We investigated the phenotypic responses of an artificial population of K. obovata, with plants transplanted from different source populations, to extremely low temperatures during winter of 2015–2016 in Yueqing County (28°20′N), Zhejiang Province of China. Using two binary traits, “with/without leaves alive on the branches” and “with/without alive buds on the tips of branches,” we classified plants in this artificial population into strong, moderate and poor cold resistance groups. We further assessed the genetic diversity, structure and differentiation of these three groups, as well as five natural populations along a latitudinal gradient using ten nuclear and six plastid microsatellite markers. Microsatellite data revealed genetic differentiation among the natural populations along the latitudinal gradient. Molecular data indicated that the cold tolerance of three groups in the artificial population was associated with their geographic origins, and that the most cold-tolerant group came from the northernmost natural population. Our study thus indicates that natural populations of K. obovata may have evolved divergent capacity of cold tolerance.
Effect of Stomatal Morphology on Leaf Photosynthetic Induction Under Fluctuating Light in Rice
Authors: Xiong Zhuang , Dun Zhigang , Wang Yucheng , Yang Desheng , Xiong Dongliang , Cui Kehui , Peng Shaobing , Huang Jianliang
Published: February 2022

Plants are often confronted with light fluctuations from seconds to minutes due to altering sun angles, mutual shading, and clouds under natural conditions, which causes a massive carbon loss and water waste. The effect of stomatal morphology on the response of leaf gas exchange to fluctuating light remains disputable. In this study, we investigated the differences in leaf stomatal morphology and photosynthetic induction across twelve rice genotypes after a stepwise increase in light intensity. A negative correlation was observed between stomatal size and density across rice genotypes. Smaller and denser stomata contributed to a faster stomatal response under fluctuating light. Plants with faster stomatal opening also showed faster photosynthetic induction and higher biomass accumulation but lower intrinsic water use efficiency (iWUE) under fluctuating light. Moreover, stomatal morphology seemed to have less effect on the initial and final stomatal conductance, and there was a minimal correlation between steady-state and non-steady-state stomatal conductance among different rice genotypes. These results highlight the important role of stomatal morphology in regulating photosynthetic efficiency and plant growth under fluctuating light conditions. To simultaneously enhance leaf iWUE when improving the photosynthetic efficiency under fluctuating light, it may be necessary to take biochemical processes into account in the future.
Synergism Between Water Management and Phosphorus Supply Enhances the Nodulation and Root Growth and Development of Chinese Milk Vetch (Astragalus sinicus L.)
Authors: Sun Zhengguo , Yi Mingxuan , Liu Xinbao , Yixin Shen , Li Jianlong
Published: February 2022

The response of root development and nodule formation of the manure crop Chinese milk vetch to different levels of soil moisture and phosphorous (P) fertilizer remains unclear. In this study, a pot experiment was performed to trace the root growth and nodule formation of Chinese milk vetch at the seedling, branching and full-flowering stages, under various soil moisture gradients [25% (W1), 50% (W2), 75% (W3), and 100% (W4) of the maximum field water-holding capacity] and P levels [0 (P0), 30 (P1), 60 (P2), and 90 (P3) kg hm–2]. The root/shoot ratio, root vitality, number of nodules, nodule weight, and nitrogenase activity were affected remarkably by soil moisture or the level of added P across the whole stage. Differences were found in the interaction effect between soil moisture and added P on the characteristic indices of the root and nodule at the different growth stages. There were obvious differences in root activity and nitrogenase activity at seedling stage, but no evident differences were found in other indices. Certain differences were also found in the indicators mentioned above at the branching stage. W1P0 and W2P0 showed the highest root/shoot ratio, W2P2 and W3P2 resulted in the highest root activity; W3P3 and W3P2 had the highest number and weight of nodules; and W3P2, W2P2, and W3P1 had higher nitrogenase activity than the other treatments at the full-flowering stage. The application of P at 60 kg hm–2and the relative soil moisture of 75% was the best P-water combination suitable for the root development, nodule formation, and nitrogen fixation of Chinese milk vetch. This study will provide a theoretical basis for the production of this plant by managing the synergistic interaction between P fertilizer and soil moisture.
The FtsH-Inactive Protein FtsHi5 Is Required for Chloroplast Development and Protein Accumulation in Chloroplasts at Low Ambient Temperature in Arabidopsis
Authors: Li Jin-Yu , Sun Jing-Liang , Tian Ying-Ying , Liu Jian-Xiang
Published: February 2022

Chloroplasts are indispensable for higher plants. The growth and development of plants are very sensitive to environmental temperature changes, and chloroplast development is also regulated by adverse environmental temperatures. However, the molecular mechanism of how plants coordinate chloroplast development and environmental temperature changes remains largely unknown. Here, a temperature-conditioned chloroplast development defective mutant thermo-sensitive mutant in leaf color 2 (tsl2) of Arabidopsis was obtained through a forward genetic screening. The tsl2 mutant showed a weak yellowish phenotype at normal growth temperature (22°C), and the phenotype was more pronounced at low growth temperature (16°C) and largely rescued at high growth temperature (29°C). Bulk Segregant Analysis (BSA) revealed that TSL2 encodes FtsH-Inactive Protein 5 (FtsHi5). Genetic complementation analysis confirmed that complemented expression of FtsHi5 rescued the chlorophyll content and thylakoid development defects observed in tsl2 mutants at 16°C. Quantitative mass spectrometry analysis with Tandem Mass Tag (TMT) isobaric labeling revealed broad changes in the chloroplast proteome of tsl2 mutant plants at low temperature, which is agreed with the impaired chloroplast biogenesis and function in tsl2 plants. Together, our data demonstrates that FtsHi5/TSL2 plays an important role in chloroplast development and protein accumulation in chloroplasts, especially at low environmental temperatures in Arabidopsis.
Background-Aware Domain Adaptation for Plant Counting
Authors: Shi Min , Li Xing-Yi , Lu Hao , Cao Zhi-Guo
Published: February 2022

Deep learning-based object counting models have recently been considered preferable choices for plant counting. However, the performance of these data-driven methods would probably deteriorate when a discrepancy exists between the training and testing data. Such a discrepancy is also known as the domain gap. One way to mitigate the performance drop is to use unlabeled data sampled from the testing environment to correct the model behavior. This problem setting is also called unsupervised domain adaptation (UDA). Despite UDA has been a long-standing topic in machine learning society, UDA methods are less studied for plant counting. In this paper, we first evaluate some frequently-used UDA methods on the plant counting task, including feature-level and image-level methods. By analyzing the failure patterns of these methods, we propose a novel background-aware domain adaptation (BADA) module to address the drawbacks. We show that BADA can easily fit into object counting models to improve the cross-domain plant counting performance, especially on background areas. Benefiting from learning where to count, background counting errors are reduced. We also show that BADA can work with adversarial training strategies to further enhance the robustness of counting models against the domain gap. We evaluated our method on 7 different domain adaptation settings, including different camera views, cultivars, locations, and image acquisition devices. Results demonstrate that our method achieved the lowest Mean Absolute Error on 6 out of the 7 settings. The usefulness of BADA is also supported by controlled ablation studies and visualizations.
Abundance of Major Cell Wall Components in Natural Variants and Pedigrees of Populus trichocarpa
Authors: Harman-Ware Anne E. , Happs Renee M. , Macaya-Sanz David , Doeppke Crissa , Muchero Wellington , DiFazio Stephen P.
Published: February 2022

The rapid analysis of biopolymers including lignin and sugars in lignocellulosic biomass cell walls is essential for the analysis of the large sample populations needed for identifying heritable genetic variation in biomass feedstocks for biofuels and bioproducts. In this study, we reported the analysis of cell wall lignin content, syringyl/guaiacyl (S/G) ratio, as well as glucose and xylose content by high-throughput pyrolysis-molecular beam mass spectrometry (py-MBMS) for >3,600 samples derived from hundreds of accessions of Populus trichocarpa from natural populations, as well as pedigrees constructed from 14 parents (7 × 7). Partial Least Squares (PLS) regression models were built from the samples of known sugar composition previously determined by hydrolysis followed by nuclear magnetic resonance (NMR) analysis. Key spectral features positively correlated with glucose content consisted of m/z 126, 98, and 69, among others, deriving from pyrolyzates such as hydroxymethylfurfural, maltol, and other sugar-derived species. Xylose content positively correlated primarily with many lignin-derived ions and to a lesser degree with m/z 114, deriving from a lactone produced from xylose pyrolysis. Models were capable of predicting glucose and xylose contents with an average error of less than 4%, and accuracy was significantly improved over previously used methods. The differences in the models constructed from the two sample sets varied in training sample number, but the genetic and compositional uniformity of the pedigree set could be a potential driver in the slightly better performance of that model in comparison with the natural variants. Broad-sense heritability of glucose and xylose composition using these data was 0.32 and 0.34, respectively. In summary, we have demonstrated the use of a single high-throughput method to predict sugar and lignin composition in thousands of poplar samples to estimate the heritability and phenotypic plasticity of traits necessary to develop optimized feedstocks for bioenergy applications.
Diversification Slowdown in the Cirrhopetalum Alliance (Bulbophyllum, Orchidaceae): Insights From the Evolutionary Dynamics of Crassulacean Acid Metabolism
Authors: Hu Ai-Qun , Gale Stephan W. , Liu Zhong-Jian , Fischer Gunter A. , Saunders Richard M. K.
Published: February 2022

Evolutionary slowdowns in diversification have been inferred in various plant and animal lineages. Investigation based on diversification models integrated with environmental factors and key characters could provide critical insights into this diversification trend. We evaluate diversification rates in the Cirrhopetalum alliance (Bulbophyllum, Orchidaceae subfam. Epidendroideae) using a time-calibrated phylogeny and assess the role of Crassulacean acid metabolism (CAM) as a hypothesised key innovation promoting the spectacular diversity of orchids, especially those with an epiphytic habit. An explosive early speciation in the Cirrhopetalum alliance is evident, with the origin of CAM providing a short-term advantage under the low atmospheric CO2 concentrations (pCO2) associated with cooling and aridification in the late Miocene. A subsequent slowdown of diversification in the Cirrhopetalum alliance is possibly explained by a failure to keep pace with pCO2 dynamics. We further demonstrate that extinction rates in strong CAM lineages are ten times higher than those of C3 lineages, with CAM not as evolutionarily labile as previously assumed. These results challenge the role of CAM as a “key innovation” in the diversification of epiphytic orchids.
GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
Authors: Mu Ruolan , Lyu Xiangguang , Ji Ronghuan , Liu Jun , Zhao Tao , Li Hongyu , Liu Bin
Published: February 2022

Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered mutations targeting the six GmBIC genes in soybean. We showed that the GmBICs overexpression (GmBICs-OX) lines strongly promoted stem elongation, while the single, double, and quadruple mutations in the GmBIC genes resulted in incremental dwarfing phenotypes. Furthermore, overexpression of GmBIC2a abolished the low blue light (LBL)-induced stem elongation, demonstrating the involvement of GmBICs in regulating cryptochrome-mediated LBL-induced shade avoidance syndrome (SAS). The Gmbic1a1b2a2b quadruple mutant displayed reduced stem elongation under LBL conditions, which was reminiscent of the GmCRY1b-OX lines. Taken together, this study provided essential genetic resources for elucidating GmBICs functional mechanisms and breeding of shade-tolerant soybean cultivars in future.
Simulation of Wheat Response to Future Climate Change Based on Coupled Model Inter-Comparison Project Phase 6 Multi-Model Ensemble Projections in the North China Plain
Authors: Bai Huizi , Xiao Dengpan , Wang Bin , Liu De Li , Tang Jianzhao
Published: February 2022

Global climate change results in more extreme temperature events, which poses a serious threat to wheat production in the North China Plain (NCP). Assessing the potential impact of temperature extremes on crop growth and yield is an important prerequisite for exploring crop adaptation measures to deal with changing climate. In this study, we evaluated the effects of heat and frost stress during wheat sensitive period on grain yield at four representative sites over the NCP using Agricultural Production System Simulator (APSIM)-wheat model driven by the climate projections from 20 Global Climate Models (GCMs) in the Coupled Model Inter-comparison Project phase 6 (CMIP6) during two future periods of 2031–2060 (2040S) and 2071–2100 (2080S) under societal development pathway (SSP) 245 and SSP585 scenarios. We found that extreme temperature stress had significantly negative impacts on wheat yield. However, increased rainfall and the elevated atmospheric CO2 concentration could partly compensate for the yield loss caused by extreme temperature events. Under future climate scenarios, the risk of exposure to heat stress around flowering had no great change but frost risk in spring increased slightly mainly due to warming climate accelerating wheat development and advancing the flowering time to a cooler period of growing season. Wheat yield loss caused by heat and frost stress increased by −0.6 to 4.2 and 1.9–12.8% under SSP585_2080S, respectively. We also found that late sowing and selecting cultivars with a long vegetative growth phase (VGP) could significantly compensate for the negative impact of extreme temperature on wheat yields in the south of NCP. However, selecting heat resistant cultivars in the north NCP and both heat and frost resistant cultivars in the central NCP may be a more effective way to alleviate the negative effect of extreme temperature on wheat yields. Our findings showed that not only heat risk should be concerned under climate warming, but also frost risk should not be ignored.
Cell Death Signaling From Endoplasmic Reticulum Stress: Plant-Specific and Conserved Features
Authors: Simoni Eduardo B. , Oliveira Célio C. , Fraga Otto T. , Reis Pedro A. B. , Fontes Elizabeth P. B.
Published: February 2022

The endoplasmic reticulum (ER) stress response is triggered by any condition that disrupts protein folding and promotes the accumulation of unfolded proteins in the lumen of the organelle. In eukaryotic cells, the evolutionarily conserved unfolded protein response is activated to clear unfolded proteins and restore ER homeostasis. The recovery from ER stress is accomplished by decreasing protein translation and loading into the organelle, increasing the ER protein processing capacity and ER-associated protein degradation activity. However, if the ER stress persists and cannot be reversed, the chronically prolonged stress leads to cellular dysfunction that activates cell death signaling as an ultimate attempt to survive. Accumulating evidence implicates ER stress-induced cell death signaling pathways as significant contributors for stress adaptation in plants, making modulators of ER stress pathways potentially attractive targets for stress tolerance engineering. Here, we summarize recent advances in understanding plant-specific molecular mechanisms that elicit cell death signaling from ER stress. We also highlight the conserved features of ER stress-induced cell death signaling in plants shared by eukaryotic cells.
Governance Strategies in Precolonial Central Mexico
Authors: Carballo David M.
Published: February 2022

Among the Indigenous polities of precolonial Mesoamerica, the Aztec empire, headed by a confederation of three city-states, was the largest recorded and remains the best understood, due to its chronicling in Spanish and Nahuatl texts following the Spanish-Aztec war and colonial transformation to New Spain. Yet its political organization is routinely mischaracterized in popular media, and lesser-known contemporaries and predecessors in central Mexico exhibit variability in governing strategies over time and space of interest to comparatively oriented scholars of premodern polities. Common themes in governance tended to draw from certain socio-technological realities and shared ontologies of religion and governing ideologies. Points of divergence can be seen in the particular entanglements between political economies and the settings and scales of collective action. In this paper, I review how governance varied synchronically and diachronically in central Mexico across these axes, and especially in relation to resource dilemmas, fiscal financing, the relative strength of corporate groups versus patron-client networks, and how rulership was legitimated.
Laterality in Emotional Language Processing in First and Second Language
Authors: Heyrani Raheleh , Nejati Vahid , Abbasi Sara , Hartwigsen Gesa
Published: February 2022

Language is a cognitive function that is asymmetrically distributed across both hemispheres, with left dominance for most linguistic operations. One key question of interest in cognitive neuroscience studies is related to the contribution of both hemispheres in bilingualism. Previous work shows a difference of both hemispheres for auditory processing of emotional and non-emotional words in bilinguals and monolinguals. In this study, we examined the differences between both hemispheres in the processing of emotional and non-emotional words of mother tongue language and foreign language. Sixty university students with Persian mother tongue and English as their second language were included. Differences between hemispheres were compared using the dichotic listening test. We tested the effect of hemisphere, language and emotion and their interaction. The right ear (associated with the left hemisphere) showed an advantage for the processing of all words in the first language, and positive words in the second language. Overall, our findings support previous studies reporting left-hemispheric dominance in late bilinguals for processing auditory stimuli.
The Values Supporting the Creativity of Employees
Authors: Balková Miluše , Lejsková Pavla , Ližbetinová Lenka
Published: February 2022

In Industry 4.0 completely new production worlds are emerging, where robots are becoming a key element and where common human skill activities and thinking are commonly surpassed. The growing degree of automation and the interconnection of the digital and the real-world create an environment that requires a set of interdisciplinary skills. For the sustainability of enterprises in this environment, human creativity acquires an irreplaceable role. The aim is to compare the application of selected values in corporate culture, which creates a space for increasing the creativity of employees from the perspective of different sizes of enterprises. The research sample consists of 1,716 companies of the Czech and Slovakia that participated in the questionnaire survey. The results of the independentT-test confirmed significant differences in the applied values between the countries being compared. Differences in terms of business size have been further explored through the ANOVA test and the Tukey HSD test. The results subsequently confirmed that the values: work meaningfulness, passion for work, and trust are applied more significantly to microenterprises in both the countries, creating an environment that supports creativity.
Resilience and Stress as Mediators in the Relationship of Mindfulness and Happiness
Authors: Bajaj Badri , Khoury Bassam , Sengupta Santoshi
Published: February 2022

The aim of the present study was to examine the mediation effects of resilience and stress, two perceived opposite constructs, in the relationship between mindfulness and happiness. Mindful Attention Awareness Scale, Connor–Davidson Resilience Scale, Subjective Happiness Scale, Depression Anxiety Stress Scales short version-21 were administered to 523 undergraduate university students in India. Structural Equation Modeling with bootstrapping was applied to test the mediating effects of resilience and stress. Results showed that resilience and stress partially mediated the mindfulness-happiness relationship. In addition, resilience partially mediated the relationship of mindfulness to stress. Findings suggest that mindfulness may play an influential role in enhancing happiness through the mediating effects of resilience and stress.
Perceived Information Overload and Unverified Information Sharing on WeChat Amid the COVID-19 Pandemic: A Moderated Mediation Model of Anxiety and Perceived Herd
Authors: Huang Qing , Lei Sihan , Ni Binbin
Published: February 2022

Individuals’ unverified information sharing on social media, namely, sharing information without verification, is a major cause of the widespread misinformation amid the COVID-19 pandemic. The association between perceived information overload and unverified information sharing has been well documented in the cognitive overload approach. However, little is known about the underlying mechanism of this process. This study aims to explore the mediating role of anxiety and the moderating role of perceived herd between perceived information overload and unverified information sharing on WeChat. Anxiety demonstrates people’s emotional response to the pandemic, whereas perceived herd describes a willingness to share certain information if it has been shared by many. The results of an online survey in China (N = 525) showed that perceived information overload was positively associated with unverified information sharing. In addition, this relationship was partially mediated by anxiety. Moreover, perceived herd positively moderated the link between anxiety and unverified information sharing, such that the indirect effect of perceived information overload on unverified information sharing via anxiety was significant in conditions where the level of perceived herd was high, whereas the indirect effect was not significant in conditions where the level of perceived herd was low. The moderated mediation model extends the cognitive overload approach and indicates that unverified information sharing is not only an individual strategy to cope with information overload but also a herding behavior to manage anxiety. Practical implications for curbing people’s tendencies toward unverified information sharing on social media are discussed.
Trajectories of Mental Health Status Among Police Recruits in Sweden
Authors: Emsing Mikael , Padyab Mojgan , Ghazinour Mehdi , Hurtig Anna-Karin
Published: February 2022

Background

The stressful and complex nature of police work and its adverse effects on mental health are well-documented in police research. The mental health of police students however, has not been given the same attention. To the best of our knowledge, studies on the mental health of Swedish police recruits have not been undertaken since 2010.

Objectives

The present study aims to examine whether there are differences in the mental health between two cohorts (2009 and 2020) of Swedish police recruits, as well as to compare the mental health of both cohorts with the general population data collected in 2002.

Methods

Data was collected using the SCL-90-R survey. Data was analyzed using multivariate analysis of variance (MANOVA) and independent sample T-tests. Bi-variate analyses including t-test and chi-square were used to examine differences in sociodemographic variables between the two cohorts.

Results

A total of 376 police recruits participated in the study. Results indicated no significant differences between the cohorts with regards to the three global indices of the SCL-90-R: Global Severity Index (GSI), Positive Symptom Total (PST), and Positive Symptom Distress Index (PSDI). Recruits with a college degree had lower scores on GSI and PSDI, similar to respondents that where in a relationship vs. singles. A total of 15 (four female) recruits had GSI scores above the Swedish patient mean. Compared with the general population, males and females from the 2009, as well as females from the 2020 cohorts had lower or insignificantly different mean scores on all global indices, with males from the 2020 cohort having a significantly lower PST score.

Conclusions

While the vast majority of recruits had results that where indicative of a low prevalence and intensity with regards to mental health disorders, some recruits did score above the Swedish patient mean. While mental preparedness is part of the curriculum for Swedish police recruits, interventions targeting the stigmas of poor mental health could be of value. The fact that educational attainment appears to have a positive impact on the mental health of police recruits, could be taken in to consideration when recruiting future police officers.
The Gut-Brain-Immune Axis in Autism Spectrum Disorders: A State-of-Art Report
Authors: Puricelli Chiara , Rolla Roberta , Gigliotti Luca , Boggio Elena , Beltrami Eleonora , Dianzani Umberto , Keller Roberto
Published: February 2022

The interest elicited by the large microbial population colonizing the human gut has ancient origins and has gone through a long evolution during history. However, it is only in the last decades that the introduction of high-throughput technologies has allowed to broaden this research field and to disentangle the numerous implications that gut microbiota has in health and disease. This comprehensive ecosystem, constituted mainly by bacteria but also by fungi, parasites, and viruses, is proven to be involved in several physiological and pathological processes that transcend the intestinal homeostasis and are deeply intertwined with apparently unrelated body systems, such as the immune and the nervous ones. In this regard, a novel speculation is the relationship between the intestinal microbial flora and the pathogenesis of some neurological and neurodevelopmental disorders, including the clinical entities defined under the umbrella term of autism spectrum disorders. The bidirectional interplay has led researchers to coin the term gut-brain-immune system axis, subverting the theory of the brain as an immune-privileged site and underscoring the importance of this reciprocal influence already from fetal life and especially during the pre- and post-natal neurodevelopmental process. This revolutionary theory has also unveiled the possibility to modify the gut microbiota as a way to treat and even to prevent different kinds of pathologies. In this sense, some attempts have been made, ranging from probiotic administration to fecal microbiota transplantation, with promising results that need further elaboration. This state-of-art report will describe the main aspects regarding the human gut microbiome and its specific role in the pathogenesis of autism and its related disorders, with a final discussion on the therapeutic and preventive strategies aiming at creating a healthy intestinal microbial environment, as well as their safety and ethical implications.
Association Between Childhood Maltreatment, FKBP5 Gene Methylation, and Anxiety Symptoms Among Chinese Adolescents: A Nested Case-Control Study
Authors: Lai Wenjian , Li Wenyan , Du Xueying , Guo Yangfeng , Wang Wanxin , Guo Lan , Lu Ciyong
Published: February 2022

Background

Anxiety symptoms are common mental health problems among adolescents worldwide. This study aimed to explore (1) the longitudinal association between childhood maltreatment and anxiety symptoms, (2) the association between childhood maltreatment and DNA methylation of the FKBP5 gene, and (3) the association of DNA methylation of the FKBP5 gene with anxiety symptoms at follow-up.

Methods

A nested case-control design was conducted to identify a case group and control group from a longitudinal study of adolescents aged 13–18 years in Guangzhou from 2019 to 2020. Adolescents with anxiety symptoms at baseline and follow-up were considered the case group, while those without anxiety symptoms at baseline and follow-up were considered the control group. The case and control groups were matched according to age and sex. Our study finally included 97 cases and 141 controls.

Results

After adjusting for significant covariates, childhood emotional abuse was associated with subsequent anxiety symptoms (β = 0.146, 95% CI = 0.010~0.283); students with physical and emotional neglect were more likely to get a lower level of DNA methylation at most CpG units of FKBP5 gene (P < 0.05); FKBP5-12 CpG 15 methylation was associated with anxiety symptoms at follow-up (β = −0.263, 95% CI = −0.458~-0.069). However, after multiple hypothesis testing, childhood maltreatment was not associated with FKBP5 DNA methylation (q > 0.10); FKBP5 DNA methylation did not show an association with subsequent anxiety symptoms (q > 0.10).

Conclusions

Childhood emotional abuse was associated with an increased risk of anxiety symptoms among Chinese adolescents. After multiple hypothesis testing, childhood maltreatment was not significantly associated with FKBP5 DNA methylation. DNA methylation of the promoter region of the FKBP5 gene was not a significant predictor of anxiety symptoms. More attention should be paid to the mental health of adolescents with childhood maltreatment.
Self-Medication of ADHD Symptoms: Does Caffeine Have a Role?
Authors: Ágoston Csilla , Urbán Róbert , Horváth Zsolt , van den Brink Wim , Demetrovics Zsolt
Published: February 2022

Objective

Stimulants are the most effective treatment for Attention Deficit/ Hyperactivity disorder (ADHD). In addition, studies have shown that nicotine dependence in patients with ADHD is probably best explained by self-medication. The question is whether this is also true for caffeine use and caffeine dependence. The aim of our study was, therefore, to examine the relationship of ADHD symptoms, caffeine consumption, caffeine use disorder (CUD) and well-being. We hypothesized that those who have more ADHD symptoms and regularly consume caffeine have higher psychological well-being than those who have more ADHD symptoms, but do not consume caffeine.

Methods

A general population sample (N = 2,259, 70.5% male, mean age 34.0) filled out the 10-item Caffeine Use Disorder Questionnaire (CUDQ), the Adult ADHD Self-report Scale (ASRS) and the WHO-5 Well-Being Index (WHO-5) and were asked about their caffeine consumption habits in an online survey.

Results

There were no associations between ADHD and coffee, tea, energy drink or cola consumption or daily caffeine consumption. However, the results of the path analysis showed that the level of ADHD symptoms was positively associated with the level of CUD (β = 0.350) and negatively with the WHO-5 (β = −0.259).

Conclusions

Caffeine consumption was not associated with ADHD symptom severity and thus not likely to represent self-medication. On the contrary, caffeine use disorder severity is associated with more ADHD symptoms and both caffeine use disorder and ADHD are associated with lower well-being.