bluuebunny/crossref_metadata_2025 huggingface dataset:
Autistic adults exhibit holistic face processing: evidence from inversion and composite face effects
Authors: Hsiung En-Yun , Chien Sarina Hui-Lin
Published: November 2024

Holistic processing is commonly measured by the face inversion effect (FIE) and the composite face effect (CFE). Previous studies examining whether individuals with autism spectrum disorder (ASD) employ holistic processing using either FIE or CFE have reported inconclusive results. By adopting a customized composite face paradigm, the present study aims to simultaneously assess both the inversion and the composite effects of holistic processing in autistic and neurotypical adults. We tested 24 adults with ASD and 24 neurotypical (NT) adults matched in age, gender, and years of education. Participants viewed sequentially presented composite faces in three Presentation Modes (aligned, inverted, and misaligned) with three Stimuli Conditions (same, composite, and different) and judged whether the top half was the same. For the dependent variables, we calculated a “performance index” in the form of the accuracy/response time of each stimuli condition in each presentation mode. The FIE and CFE were computed to index the magnitude of holistic processing. Our results showed that the NT group responded more accurately in less time than the ASD group across task conditions. Notably, both the NT and the ASD groups exhibited a significant FIE with similar magnitude. Likewise, both the NT and the ASD groups showed a greater-than-zero CFE. Moreover, individuals’ CFE positively correlated with FIE and negatively correlated with the AQ scores for all participants. In summary, individuals with ASD exhibit holistic processing when viewing faces, evidenced by the presence of both FIE and CFE and the positive correlations between the two effects.
Real-time multicompartment Hodgkin-Huxley neuron emulation on SoC FPGA
Authors: Beaubois Romain , Cheslet Jérémy , Ikeuchi Yoshiho , Branchereau Pascal , Levi Timothee
Published: November 2024

Advanced computational models and simulations to unravel the complexities of brain function have known a growing interest in recent years in the field of neurosciences, driven by significant technological progress in computing platforms. Multicompartment models, which capture the detailed morphological and functional properties of neural circuits, represent a significant advancement in this area providing more biological coherence than single compartment modeling. These models serve as a cornerstone for exploring the neural basis of sensory processing, learning paradigms, adaptive behaviors, and neurological disorders. Yet, the high complexity of these models presents a challenge for their real-time implementation, which is essential for exploring alternative therapies for neurological disorders such as electroceutics that rely on biohybrid interaction. Here, we present an accessible, user-friendly, and real-time emulator for multicompartment Hodgkin-Huxley neurons on SoC FPGA. Our system enables real-time emulation of multicompartment neurons while emphasizing cost-efficiency, flexibility, and ease of use. We showcase an implementation utilizing a technology that remains underrepresented in the current literature for this specific application. We anticipate that our system will contribute to the enhancement of computation platforms by presenting an alternative architecture for multicompartment computation. Additionally, it constitutes a step toward developing neuromorphic-based neuroprostheses for bioelectrical therapeutics through an embedded real-time platform running at a similar timescale to biological networks.
A three-classification model for identifying migraine with right-to-left shunt using lateralization of functional connectivity and brain network topology: a resting-state fMRI study
Authors: Nie Weifang , Zeng Weiming , Yang Jiajun , Wang Lei , Shi Yuhu
Published: November 2024

Introduction

Right-to-left shunting has been significantly associated with migraine, although the neural mechanisms remain complex and not fully elucidated. The aim of this study was to investigate the variability of brain asymmetry in individuals with migraine with right-to-left shunting, migraine without right-to-left shunting and normal controls using resting-state fMRI technology and to construct a three-classification model.

Methods

Firstly, asymmetries in functional connectivity and brain network topology were quantified to laterality indices. Secondly, the laterality indices were employed to construct a three-classification model using decision tree and random forest algorithms. Ultimately, through a feature score analysis, the key brain regions that contributed significantly to the classification were extracted, and the associations between these brain regions and clinical features were investigated.

Results

Our experimental results showed that the initial classification accuracy reached 0.8961. Subsequently, validation using an independent sample set resulted in a classification accuracy of 0.8874. Further, after expanding the samples by the segmentation strategy, the classification accuracies were improved to 0.9103 and 0.9099. Additionally, the third sample set yielded a classification accuracy of 0.8745. Finally, 9 pivotal brain regions were identified and distributed in the default network, the control network, the visual network, the limbic network, the somatomotor network and the salience/ventral attention network.

Discussion

The results revealed distinct lateralization features in the brains of the three groups, which were closely linked to migraine and right-to-left shunting symptoms and could serve as potential imaging biomarkers for clinical diagnosis. Our findings enhanced our understanding of migraine and right-to-left shunting mechanisms and offered insights into assisting clinical diagnosis.
Efficacy and safety of Latilactobacillus curvatus LB-P9 on hair health: a randomized, double-blind, placebo-controlled clinical trial
Authors: Choi Sun Young , Ko Eun Jung , Seok Joon , Han Hye Sung , Yoo Kwang Ho , Song MiKyung , Song Kyoungsub , Kim Beom Joon
Published: November 2024

Introduction

Numerous factors influence hair health, including genetic predisposition, hormonal changes, stress, nutritional deficiencies, medical conditions, or medications. With the rising interest in maintaining hair health, alternative approaches such as functional cosmetics and food products are gaining attention. Probiotics, health-beneficial live microorganisms, are emerging as potential candidates for improving hair health. Therefore, this study aimed to evaluate the effects and safety of oral intake of Latilactobacillus curvatus LB-P9 on hair health.

Methods

The study was a randomized, double-blind, placebo-controlled clinical trial involving participants (aged 18–60 years old) with mild to moderate hair damage. Participants were randomly assigned to the test (receiving LB-P9 supplements) or control (receiving a placebo) groups, respectively. Efficacy was assessed using measures such as hair luster, elasticity, and participant satisfaction. Safety evaluations comprised physical examinations, vital sign measurements, laboratory tests, and observation of adverse reactions.

Results

Overall, 80 participants were enrolled in the trial. Significant improvements were observed in hair luster, elasticity, and participant satisfaction in the test group compared to the control group. In the test group, the hair luster parameter increased by 1.65 ± 2.30 (LBNT) at 24 weeks (p < 0.001), indicating a 19% improvement over the control group. Subgroup analysis revealed significant improvement in hair luster among females with short hair. Additionally, hair tensile strength, reflecting hair elasticity and participant satisfaction are increased by 10.27 ± 16.40 (gf/mm2) at 24 weeks (p = 0.001) in the test group. The subjective indicator of participant satisfaction, which improves as survey scores decrease, significantly decreased in the test group by −17.81 ± 14.35 points (p < 0.001) after 24 weeks of consuming the test food than before consuming it. No significant adverse reactions were reported, and safety evaluations indicated no adverse effects linked to LB-P9 consumption.

Conclusion

Probiotics, including LB-P9, may serve as an alternative in the management of hair health. The findings of this study support the possible benefits of LB-P9 supplementation in enhancing hair luster and elasticity.
Association of phenylalanine and tyrosine metabolism with mortality and response to nutritional support among patients at nutritional risk: a secondary analysis of the randomized clinical trial EFFORT
Authors: Buchmueller Lena C. , Wunderle Carla , Laager Rahel , Bernasconi Luca , Neyer Peter J. , Tribolet Pascal , Stanga Zeno , Mueller Beat , Schuetz Philipp
Published: November 2024

Background

Elevated phenylalanine serum level is a surrogate marker of whole-body proteolysis and has been associated with increased mortality in critically ill patients. Tyrosine is a metabolite of phenylalanine and serves as a precursor of thyroid hormones and catecholamines with important functions in the oxidative stress response among others. Herein, we examined the prognostic significance of phenylalanine, tyrosine, as well as its metabolites nitrotyrosine, L-3,4-dihydroxyphenylalanine (DOPA), and dopamine regarding clinical outcomes and response to nutritional therapy in patients at nutritional risk.

Methods

This is a secondary analysis of the Effect of Early Nutritional Support on Frailty, Functional Outcomes, and Recovery of Malnourished Medical Inpatients Trial (EFFORT), a randomized controlled trial investigating individualized nutritional support compared to standard care in patients at risk of malnutrition. The primary outcome was 30-day all-cause mortality.

Results

We analyzed data of 238 patients and found a significant association between low plasma levels of phenylalanine [adjusted HR 2.27 (95% CI 1.29 to 3.00)] and tyrosine [adjusted HR 1.91 (95% CI 1.11 to 3.28)] with increased 30-day mortality. This association persisted over a longer period, extending to 5 years. Additionally, trends indicated elevated mortality rates among patients with low nitrotyrosine and high DOPA and dopamine levels. Patients with high tyrosine levels showed a more pronounced response to nutritional support compared to patients with low tyrosine levels (HR 0.45 versus 1.46, p for interaction = 0.02).

Conclusion

In medical inpatients at nutritional risk, low phenylalanine and tyrosine levels were associated with increased short-and long-term mortality and patients with high tyrosine levels showed a more pronounced response to nutritional support. Further research is warranted to gain a deeper understanding of phenylalanine and tyrosine pathways, their association with clinical outcomes in patients at nutritional risk, as well as their response to nutritional therapy.

Clinical trial registration

www.clinicaltrials.gov, identifier NCT02517476.
Efficacy and safety of first-line immune checkpoint inhibitor combination therapies in patients with advanced esophageal squamous cell carcinoma: a network meta-analysis
Authors: Wang Chenglong , Cai Tongze , Wei Jiangcun , Huang Ying , Xiao Lin , Li Tong , Qin Zujie
Published: November 2024

Background

We performed a network meta-analysis of phase III trials to compare the efficacy and safety of first-line regimens for patients with advanced esophageal squamous cell carcinoma (ESCC).

Methods

A systematic review and Bayesian network meta-analysis were conducted by retrieving relevant literature from PubMed, Embase, the Cochrane Library, and the Web of Science. We included published sources of randomized clinical trials comparing immunotherapy combinations for treating advanced ESCC.

Results

We analyzed seven studies involving eight immunotherapy combinations and 4688 patients. For patients without programmed death-ligand 1 (PD-L1) selection, it was found that the combination of toripalimab and chemotherapy provided better overall survival than chemotherapy alone (hazard ratio = 0.58, 95% confidence interval (CI) 0.43-0.78). Compared with chemotherapy alone, Sintilimab or camrelizumab plus chemotherapy seemed to achieve the best progression-free survival (hazard ratio = 0.56, 95% CI 0.46-0.68). Nivolumab plus chemotherapy appeared to provide the best objective response rate, with significant differences versus chemotherapy alone (odds ratio = 0.49, 95% CI 0.38-0.64). Nivolumab plus ipilimumab resulted in a relatively lower incidence of adverse events of grade ≥3 than other regimens.

Conclusions

The combination of immune checkpoint inhibitors (ICIs) with chemotherapy provided a high probability of more effective treatment in comparison with chemotherapy alone for patients with advanced ESCC. Toripalimab and sintilimab plus chemotherapy were ranked as providing the highest OS and PFS benefit in the first-line setting, respectively.
Efficacy and safety of regorafenib for the treatment of metastatic colorectal cancer in routine clinical practice: results from a Spanish hospital
Authors: Sotoca Rubio Pilar , Barrill Corpa Ana María , Alia Navarro Víctor , Pérez de Aguado Rodríguez Patricia , Moreno Doval Jaime , Calvo Pérez Juan Carlos , Guerrero Serrano Patricia , García Merino Carlos , García de Quevedo Suero Coral , Fernández-Fradejas Jorge , Serrano Domingo Juan José , Martínez Delfrade Íñigo , Morón García Blanca Isabel , Ferreiro Monteagudo María Reyes , de Frutos González Belén
Published: November 2024

Introduction

Regorafenib is indicated as treatment in third-line and beyond in patients with metastatic colorectal cancer.

Methods

This is a retrospective study of a cohort of patients with mCRC treated with regorafenib in Hospital Universitario Ramón y Cajal, in Madrid, Spain.

Results

With the aim to assess the efficacy and safety of regorafenib, 91 patients treated between 2013 and 2023 were included. Only 1.1% of patients achieved disease control. Median progression free survival was 2.40 months and median overall survival was 4.76 months. The most frequent adverse events were fatigue and hand-foot skin reaction (59.34% and 28.57%, respectively).

Discussion

Our results confirm the safety of regorafenib as treatment of mCRC in real clinical practice. Although our population is less pretreated than in the CORRECT trial, our disease control rate was inferior. This difference may be due to a worse baseline status and a high percentage of hepatic disease showed in our patients.
A real−world pharmacovigilance study of FDA Adverse Event Reporting System events for pralsetinib
Authors: Yin Yi , Sun Fengli , Jin Youpeng
Published: November 2024

Background

Pralsetinib, a selective oral inhibitor of rearranged during transfection (RET) fusion proteins and oncogenic RET mutants, has shown significant efficacy in treating RET fusion-positive non-small cell lung cancer and thyroid cancer. However, since pralsetinib was approved in the United States in September 2020, there have been limited reports of post-marketing adverse events (AEs). In this study, we aimed to analyze the AE signals with pralsetinib on the basis of the United States Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) to provide instructions in clinical practice.

Methods

All AE reports were obtained from the FAERS database from the first quarter (Q3) of 2020 to the second quarter (Q2) of 2024. Various signal quantification techniques were used for analysis, including reporting odds ratios, proportional reporting ratios, Bayesian confidence propagation neural network, and multi-item gamma Poisson shrinker (MGPS)-based empirical Bayesian geometric mean.

Results

Out of 8,341,673 case reports in the FAERS database, 1,064 reports of pralsetinib as the “primary suspected (PS)” AEs were recorded, covering 26 system organ classes and 256 preferred terms. Of the reports, 62.5% were from consumers rather than healthcare professionals. The most common systems were general disorders and administration site conditions (n = 704), investigations (n = 516), and gastrointestinal disorders (n = 405). A total of 95 significant disproportionality preferred terms (PTs) conformed to the four algorithms simultaneously. AEs that ranked the top three at the PT level were hypertension (n = 80), asthenia (n = 79), and anemia (n = 65). Of the 95 PTs with significant disproportionation, unexpected significant AEs such as increased blood calcitonin, increased myocardial necrosis marker, and bacterial cystitis were observed, which were not mentioned in the drug’s instructions. The median onset time of pralsetinib-associated AEs was 41 days [interquartile range (IQR) 14–86 days]. The majority of the AEs occurred in 30 days (42.86%).

Conclusion

Our pharmacovigilance analysis of real-world data from the FEARS database revealed the safety signals and potential risks of pralsetinib usage. These results can provide valuable evidence for further clinical application of pralsetinib and are important in enhancing clinical medication safety.
Effect of NLRP3 inflammasome induced astrocyte phenotype alteration in morphine tolerance
Authors: Yuan Zhenyu , Lu Boxuan , Zhang Meiling , Lu Yinxiao , Wang Zhihui , Zhang Wenhao , Cheng Hao , Wu Zhifang , Ji Qing
Published: November 2024

Introduction

Morphine is a widely used analgesic, but its prolonged use often leads to tolerance, limiting its therapeutic efficacy. Research implicates the NLRP3 inflammasome and reactive astrocytes in the development of morphine tolerance, with reactive astrocytes classified into A1 neurotoxic and A2 neuroprotective phenotypes. This study explores the role of the NLRP3 inflammasome and the transformation of astrocyte phenotypes in the progression of morphine tolerance.

Methods

A model of morphine tolerance was established by administering morphine intrathecally for seven consecutive days. To inhibit NLRP3 inflammasome activation, we coadministered MCC950, a selective NLRP3 inhibitor. Thermal withdrawal latency was used to assess tolerance development. Protein and mRNA levels of GFAP, IL-18, NLRP3, C3 (A1 marker), and S100A10 (A2 marker) in the spinal cord were measured using Western blotting (WB) and real-time quantitative polymerase chain reaction (RT-qPCR). Immunofluorescence was employed to assess the colocalization of C3 and GFAP.

Results

Seven days of morphine administration induced tolerance, which was associated with increased levels of GFAP, IL-18, NLRP3, and C3, and a decreased level of S100A10. Coadministration of morphine and MCC950 significantly slowed the development of morphine tolerance and reversed changes in NLRP3, IL-18, GFAP, C3, and S100A10 protein levels.

Discussion

Our findings indicate a significant link between NLRP3 inflammasome activation and morphine tolerance, suggesting that NLRP3 contributes to the transformation of astrocytes to the A1 phenotype. Inhibiting NLRP3 inflammasome activation holds promise in reversing astrocyte phenotype changes, potentially mitigating morphine tolerance.
Preparation and anti-colon cancer effect of a novel curcumin analogue (CA8): in vivo and in vitro evaluation
Authors: Wen Jie , Zhao Lingmao , Li Zhuohan , Pi Chao , Feng Xianhu , Shi Peng , Yang Hongru , Chen Ligang , Wang Xiaodong , Liu Furong , Wei Yumeng , Zhao Ling
Published: November 2024

Chemotherapy remains the first choice of treatment for colon cancer despite the inevitable adverse effects. Curcumin (CU) possesses antitumor activity but has poor aqueous solubility, low bioavailability, and weak activity. To address this, nine novel monocarbonyl CU analogues were designed, synthesized, and evaluated in the present study. Among them, CA8 exhibited the highest water solubility, which was approximately 2.37 × 106 times that of CU. In addition, compared with CU, its cytotoxicity on Caco-2 cells (19.2 times/48 h) was stronger. Of note, CA8 arrestedthe cell cycle of Caco-2 cells at the G2/M phase and induced apoptosis. Meanwhile, acute toxicity experiments indicated that KM mice tolerated CA8 for up to 300 mg/kg CA8 (oral administration) and 50 mg/kg CA8 (intraperitoneal injection). The oral administration of CA8 to Sprague Dawley rats exhibited higher AUC (0-t) (6.23-fold) and longer MRT (0-t) (3.35-fold) than that of CU. CA8 also inhibited the proliferation and angiogenesis of tumor cells more than CU and tegafur. Finally, CA8 may exert anti-tumor effects through the activation of JNK pathway and inhibition of AKT pathway. These results suggest that CA8 is a safe and highly effective new drug for colon cancer treatment.
Effects of loneliness on short video addiction among college students: the chain mediating role of social support and physical activity
Authors: Zhao Zhe , Kou Yali
Published: November 2024

Loneliness is a common public health problem that affects physical and mental health. Prior research has demonstrated a connection between internet addiction and loneliness. Short video addiction is a novel internet addiction. It is necessary to study the potential psychological mechanisms between loneliness and short video addiction. This study investigated the associations between loneliness and short video addiction, as well as the mediating roles played by social support and physical activity.

Methods

A sample of 388 college students was selected, and the questionnaires included the Loneliness Scale Short Version, the Short Video Addiction Scale, the Social Support Scale, and the Physical Activity Scale. The data were analyzed using SPSS for correlation analysis and PROCESS macros for mediation effect analysis.

Results

(1) Loneliness significantly positively affected short video addiction. (2) The association between loneliness and short video addiction was independently mediated by social support. (3) Physical activity independently mediated loneliness and short video addiction. (4) Social support and physical activity play a chain mediating role in the association between loneliness and short video addiction. Our research improves the literature on loneliness and short video addiction, enhances comprehension of the impacts, and offers college students effective ways to combat the addiction.
How can robots facilitate physical, cognitive, and social engagement in skilled nursing facilities?
Authors: Preston Rhian C. , Shippy Madison R. , Aldwin Carolyn M. , Fitter Naomi T.
Published: November 2024

As people live longer, the population of older adults in need of support continues to expand relative to the available workforce of caregivers, necessitating new solutions to supplement caregiver availability for the physical, cognitive, and social needs of older adults. Robotics and automation present strong possible solutions. Past solutions have typically supported short-term rehabilitation and aging in place, yet many older adults live in skilled nursing facilities (SNFs), a setting reached by relatively little research to date. In this paper, we examine the unique needs of staff and residents at SNFs, after which we begin an iterative design process of robot-mediated wellness activities for the SNF space. We worked closely with domain experts in exercise science and physical therapy for older adults and a local SNF to design and test a series of robot-mediated activity prototypes with residents, visitors, and staff. We found that while both residents and staff highly value physical activity, there are nuanced challenges associated with supporting resident activity (one important element of overall wellbeing). As a result, we considered and tested a wide range of intervention options from usual approaches (e.g., mirroring movements) to creative approaches (e.g., social engagement via lewd humor). Our final design insights can inform practitioners who wish to use robots to support resident wellbeing in SNFs.
Persistent elevation of plasma markers of cellular senescence after hip fracture: a pilot longitudinal study
Authors: Lenze Eric J. , Nicol Ginger E. , Kuchel George A. , Avidan Michael S. , Diniz Breno S.
Published: November 2024

Introduction

Hip fractures may result from and contribute to accelerated biological aging. We aimed to evaluate the impact of hip fracture and its surgery on the senescence-associated secretory phenotype (SASP) index, a composite of peripheral protein markers where higher scores are thought to indicate greater levels of cellular senescence and accelerated aging.

Methods

We examined the SASP index in plasma over 12 weeks post-surgery and its prediction of long-term post-surgical functional outcomes. We included 60 older adults: 20 recruited immediately after hip fracture surgery, and 40 comparison individuals who were either healthy or suffering chronic psychosocial stress (caregiving). We assessed 22 SASP biomarkers and calculated the SASP index score for each hip fracture participant immediately following fracture surgery and 4 and 12 weeks later. Functional recovery was assessed at 12, 26, and 52 weeks after hip replacement surgery.

Results

The hip fracture group had higher SASP index scores than the comparison groups, after adjusting for potential confounding variables (p = 0.021). SASP index scores in hip fracture patients increased further by week 4 after surgery (p < 0.001), declining by week 12 but remaining elevated overall. However, the SASP index scores were not significantly associated with functional recovery after hip replacement surgery at 26 or 52 weeks after surgery. In conclusion, after hip fracture surgery SASP scores are elevated, continue to rise over time, and do not return to normal by 12 weeks post-surgery.

Discussion

Our findings support the need to investigate this phenomenon of post-operative senescence, including whether novel interventions such as senolytics would help older adults facing major surgery.
Progressing a river health assessment framework to tropical waters
Authors: Campos Camila Aida , Tonin Alan M. , Sena Guilherme , Gonçalves Júnior José Francisco
Published: November 2024

Diverse anthropogenic activities have threatened the valuable tropical biodiversity and freshwater reserves. Despite that, there is little concern in the Brazilian legislation about the ecological integrity of aquatic ecosystems. We proposed a framework based on two joint indices, allowing the creation of a management tool. The first is the Tropical Water Healthy Index (TWHI), a river index that integrates pressures on aquatic ecosystems, their conditions, and societal/governmental response. The second is the Suitability of the Class of Use (SCU), which measures whether rivers’ conditions are in accordance with their classes of use proposed for the legal Brazilian water framework. Our results showed that most sites had good river health conditions, with the worst conditions occurring in areas with high urban occupations followed by agricultural areas. The second index indicated that few streams were poorly or incompatible with their classes of use, suggesting their classifications were based on current conditions but not future intentions. The force of these joint analyses was shown in the Melchior River stretch, which was classified as compatible with SCU, but the TWHI was critical. This is good to show that we simultaneously have a problem with the legislation and to indicate something that needs to be done quickly for managers. Therefore, we built a management model that can assess the real situation of aquatic ecosystems and define efficient management strategies, producing alerts for the decision-maker.
Large, but short-term, increase in fecal indicator bacteria following extreme flooding from Hurricane Harvey in Houston, TX
Authors: Mukherjee Maitreyee , Hossain Md Shakhawat , Boswell John , Zhang Yan , Allen Michael S. , LaMontagne Michael G. , Gentry Terry J.
Published: November 2024

Hurricane Harvey caused widespread flooding along the Texas Gulf Coast in August 2017; some areas of Houston received >150 cm of rainfall within a few days. Due to concerns over fecal contamination of floodwaters, surface water samples were collected at six locations in the southeastern Houston area immediately before and after the hurricane and then every 1 to 2 weeks thereafter over a 2-month period. Total E. coli was enumerated using the IDEXX Quanti-Tray/2000 system. DNA extracted from water samples was analyzed via quantitative real-time PCR (qPCR) for general and source-specific total Bacteroidales and human Bacteroidales markers, and digital PCR (dPCR) for antibiotic resistance genes (ARG) and a plasmid (pBI143) associated with human waste. SourceTracker2 was used to determine human source contributions based on metagenomic analysis of PCR-amplified 16S rRNA gene fragments. Samples collected immediately after the hurricane had elevated levels of E. coli, ranging from 488 to 1,733 CFU 100 ml−1. After 1 week, E. coli levels decreased to <100 MPN 100 ml−1. Total Bacteroidales numbers were elevated immediately following the hurricane and remained high for 12 days. Human-source contributions, as assessed by PCR methods and metagenomic analysis, peaked within 12 days after the hurricane consistently across all sampling sites. Multiple regression analysis of environmental parameters, copies of ARG and pBI143, and metagenomic data confirmed that human waste caused the dramatic, short-term, high levels of fecal contamination of floodwaters generated by Hurricane Harvey. Fecal indicators approached normal background levels approximately 3 weeks after the rainfall ended.
Why Early Chatter Matters: The Role of Language in Shaping Futures
Authors: McArthur Matthew , Friend Margaret
Published: November 2024

The words we learn early in life are building blocks for our brains, helping them grow and helping us understand the world better. As we learn new words and the concepts behind them, we support the foundation on which our future learning, relationships, and achievements are built. A rich early vocabulary opens the door to understanding complex ideas, solving problems, and expressing thoughts and emotions more clearly. Early language can even support distant future outcomes such as academic success in high school and employment as an adult. This article will discuss why early chatter is so powerful, how it supports future learning, and what factors are the most important contributors to developing vocabulary in the first few years of life.
Theories of change: navigating diverse expert perceptions and preferences for global food system transformation
Authors: Loring Philip A. , Loken Brent , Bhalla Iqbal S. , Daniel Adil , De La Torre Ana , Friedman Suzy , Melo-Rijk Melody , del Rosario Liezl Stuart , Tardiou Ariane , van Dooren Corné , Upadhyay Gargi
Published: November 2024

Introduction

Efforts are underway to transform food systems in light of their contributions to global challenges like climate change. However, food systems are highly complex, involve noteworthy place-based challenges, and there is often debate and disagreement among experts over appropriate technologies or interventions to prioritize. Tracking progress, and understanding these differences, is thus a critical need.

Methods

We surveyed food systems experts in eight countries about their preferences for 20 different food system transformation strategies and their sentiment regarding whether current initiatives are sufficient to meet 2030 goals for climate and biodiversity.

Results

Expert sentiment is overwhelmingly negative, and experts are concerned about multiple “transformation gaps,” including gaps in ambition, strategy, and implementation. Expert rankings for 20 strategies vary notably among countries and in ways that do not match those same experts’ rankings for the strength of the science behind each lever. Factor analysis reveals four distinct theories of change informing experts’ subjective biases: transformation via technical optimization, via smallholder support, via nature-positive solutions, and via supply chain enabling conditions.

Discussion

These findings provide insights for navigating the complexities of food system transformation and illustrate the influence on our strategies of preconceptions and biases in how we have come to understand the nature of the challenge.
Case report: Complications after using the “blind-stitch” method in a dairy cow with a left displaced abomasum: treatment, outcome, and economic evaluation
Authors: Schären-Bannert Melanie , Bittner-Schwerda Lilli , Rachidi Fanny , Starke Alexander
Published: November 2024

A fourth lactation dairy cow that was 35 days in milk was referred to the clinic for treatment after undergoing unsuccessful treatment of a left displaced abomasum (LDA). The physical examination revealed complications after using the “blind-stitch” method for correction of the LDA; the cow had an abnormal general demeanor, decreased gastrointestinal motility, and local inflammation of the abdominal wall at the site of the suture. Systemic antibiotics, anti-inflammatory drugs, and intravenous fluids were administered, and a right flank laparotomy and omentopexy according to Dirksen were performed after cutting the suture and breaking down the adhesions of the localized peritonitis between the abdominal wall and the abomasal puncture site. The cow was monitored clinically and discharged 2 weeks after referral. The cow was milked for another two lactations producing a total of 18,000 kg of milk, with a lifetime production of 59,141 kg. The total cost for the case was 310 € for the first intervention using the “blind-stitch” method and 897 € for the second laparotomic intervention. The costs (excluding tax) of both procedures including physical examination, surgery, medications, diagnostics, and labor were calculated. The lost revenue associated with the withdrawal period and lower milk production was 4,168 €. Percutaneous LDA fixation techniques, such as the “blind-stitch” and “toggle-pin” methods, have gained popularity because they are quick and cost-effective and involve minimal labor. However, many buiatricians are critical of these techniques because of the high risk of complications. The following four factors require careful consideration when choosing a method for LDA correction: (1) Percutaneous methods require precision and adherence to the described inclusion, exclusion, and cancelation criteria; (2) Operator skill is essential, and therefore regularly performing laparotomies increases surgical experience and enables the veterinarian to better manage different and more complex abdominal disorders; (3) By performing a laparotomy, other underlying abdominal disorders such as reticuloperitonitis and abomasal ulcers may be detected; (4) Postoperative husbandry and treatment are important factors affecting the outcome. The cost calculated for this case underlines the potential benefit and necessity of successful animal health management and the importance of a highly skilled veterinarian and farm workforce.
Pain Control and Opioid Consumption in Patients Undergoing Total Hip or Knee Arthroplasty Receiving a Preoperative Low Dose of Gabapentin
Authors: Fioccola Antonio , Pinto Ana Marta , Nolan Rachel , Free Ross , Tariq Wajeeha , Pozzi Tommaso , Villa Gianluca , Di Filippo Alessandro , Romagnoli Stefano , Tujjar Omar
Published: November 2024

Background: Meta-analyses and randomized controlled trials were inconclusive regarding the role of gabapentinoids in patients undergoing joint arthroplasties. The aim of the present study was to investigate the effect of a preoperative low dose of gabapentin in patients undergoing total hip (THA) and knee arthroplasties (TKA). Methods: A retrospective observational study was conducted on 135 patients undergoing THA and TKA at the National Orthopedic Hospital Cappagh, Dublin, from July to December 2022. The primary outcome was the assessment of numerical rating scores (NRS) for postoperative pain at various time intervals. Results: During the observation period, 55 patients received a preoperative dose of gabapentin, while 80 patients did not. Statistically significant differences in numerical rating scores (NRS) were found at 6 (3 vs. 0, p < 0.001), 12 (4 vs. 2, p < 0.001), 18 (4 vs. 3, p < 0.001), and 24 h (4 vs. 3, p = 0.010) after surgery, in favor of the group receiving gabapentin. A reduction in opioid consumption, measured as morphine equivalents, was also noted in the gabapentin group (40 vs. 30 mg, p = 0.040). Conclusions: A low preoperative dose of gabapentin was associated with reduced postoperative pain and opioid consumption in patients undergoing TKA and THA, without impacting hospital stay. Prospectively designed trials are encouraged to assess the safety and effect on pain control of a preoperative low dose of gabapentin.
Morphological and Performance Biomechanics Profiles of Draft Preparation American-Style Football Players
Authors: Mokha Monique , Berrocales Maria , Rohman Aidan , Schafer Andrew , Stensland Jack , Petruzzelli Joseph , Nasri Ahmad , Thompson Talia , Taha Easa , Bommarito Pete
Published: November 2024

Background/Objectives: Using advanced methodologies may enhance athlete profiling. This study profiled morphological and laboratory-derived performance biomechanics by position of American-style football players training for the draft. Methods: Fifty-five players were categorized into three groups: Big (e.g., lineman; n = 17), Big–skill (e.g., tight end; n = 11), and Skill (e.g., receiver; n = 27). Body fat (BF%), lean body mass (LBM), and total body mass were measured using a bioelectrical impedance device. Running ground reaction force (GRF) and ground contact time (GCT) were obtained using an instrumented treadmill synchronized with a motion capture system. Dual uniaxial force plates captured countermovement jump height (CMJ-JH), normalized peak power (CMJ-NPP), and reactive strength. Asymmetry was calculated for running force, GCT, and CMJ eccentric and concentric impulse (IMP). MANOVA determined between-group differences, and radar plots for morphological and performance characteristics were created using Z-scores. Results: There was a between-group difference (F(26,80) = 5.70, p < 0.001; Wilk’s Λ = 0.123, partial η2 = 0.649). Fisher’s least squares difference post hoc analyses showed that participants in the Skill group had greater JH, CMJ-NPP, reactive strength, and running GRF values versus Big players but not Big–skill players (p < 0.05). Big athletes had greater BF%, LBM, total body mass, and GCT values than Skill and Big–skill athletes (p < 0.05). Big–skill players had greater GCT asymmetry than Skill and Big players (p < 0.05). Asymmetries in running forces, CMJ eccentric, and concentric IMP were not different (p > 0.05). Morphological and performance biomechanics differences are pronounced between Skill and Big players. Big–skill players possess characteristics from both groups. Laboratory-derived metrics offer precise values of running and jumping force strategies and body composition that can aid sports science researchers and practitioners in refining draft trainee profiles.
Molecular Insights into Radiation Effects and Protective Mechanisms: A Focus on Cellular Damage and Radioprotectors
Authors: Ibáñez Blanca , Melero Ana , Montoro Alegría , San Onofre Nadia , Soriano Jose M.
Published: November 2024

Ionizing radiation has been a critical tool in various fields, such as medicine, agriculture, and energy production, since its discovery in 1895. While its applications—particularly in cancer treatment and diagnostics—offer significant benefits, ionizing radiation also poses risks due to its potential to cause molecular and cellular damage. This damage can occur through the direct ionization of biological macromolecules, such as deoxyribonucleic acid (DNA), or indirectly through the radiolysis of water, which generates reactive oxygen species (ROS) that further damage cellular components. Radioprotectors, compounds that protect against radiation-induced damage, have been extensively researched since World War II. These agents work by enhancing DNA repair, scavenging free radicals, and boosting antioxidant defenses, thereby protecting healthy tissues. Furthermore, some radioprotective agents also stimulate DNA repair mechanisms even after radiation exposure, aiding in recovery from radiation-induced damage. This article explores the molecular mechanisms of radiation-induced damage, focusing on both direct and indirect effects on DNA, and discusses the role of radioprotectors, their mechanisms of action, and recent advancements in the field. The findings underscore the importance of developing effective radioprotective strategies, particularly in medical and industrial settings, where radiation exposure is prevalent.
The Power of WNT5A and FZD3 Gene Expression and Methylation Status in the Diagnosis–Treatment–Cause Triangle in Tension-Type Headache
Authors: Kılıçaslan Ferhat , Geyik Sırma , Görücü Yılmaz Şenay
Published: November 2024

DNA methylation is the epigenetic pathway controlling cellular gene expression. Methylation is a natural and cellular epigenetic mechanism for gene silencing. The fact that the genes that the cell decides to be silent do not speak or begin to speak may coincide with diseases. For explanatory evidence, changes at the DNA level can provide realistic information. Wnt/β-catenin signaling has an important role in the pain process. For this purpose, we investigated the relationship between clinical data, wingless-type MMTV integration site family, member 5A (WNT5A), and Frizzled Class Receptor 3 (FZD3) gene methylation and expression in a cohort of tension-type headache (TTH) patients (N = 130) and healthy control (N = 117) individuals. Comorbidities were evaluated. Methylation profiling was performed using Real-Time PCR with a TaqMan primer-probe. The diagnostic power (receiver operating characteristic—ROC) was determined according to the expression and methylation status. Ultimately, WNT5A was found to be upregulated and hypermethylated, and FZD3 was found to be upregulated and hypomethylated. Finally, the area under the curve (AUC) data for FZD3 upregulation (0.983) and hypomethylation (0.866) showed diagnostic values. WNT5A and FZD3 may contribute to the pathogenesis of the disease depending on their expression and methylation profile during the TTH process. At the same time, diagnostic powers have the potential to be a resource for early treatment and new therapeutic approaches.
Perceived Risk Assessment Criteria for Public–Private Partnership Projects in the Water and Sewage Sector: Comparison of Perspectives from Iranian Public and Private Sectors
Authors: Moradi Shahdadi Leila , Aminnejad Babak , Sarvari Hadi
Published: November 2024

This research used the SWARA approach to analyze risk assessment criteria for public–private partnership (PPP) projects in Iran’s water and sewage sectors to identify and prioritize the most significant elements influencing project success from public and private viewpoints. Key results show that the public sector considers “risk probability” to be the most important aspect, highlighting the requirement for stability and predictability in project outcomes. In contrast, the private sector prioritizes the “ability to predict and discover risk”, emphasizing efficiently anticipating and managing uncertainty. Furthermore, this study revealed five common major risk characteristics, including “risk manageability” and “uncertainty of risk”; however, their rankings differ per industry, demonstrating various risk prioritizing methodologies. This study is unique in that it focuses only on Iran’s water and sewage infrastructure, an area historically neglected in PPP research, providing a rare investigation of sector-specific hazards as well as the interaction between public and private interests in a developing country environment. The paper makes specific suggestions, calling for more openness, improved communication, and the use of sophisticated risk management techniques to bridge the gap across sectors. These findings not only add to the scholarly knowledge of PPP dynamics in emerging countries but also provide practical recommendations for governments and private investors navigating Iran’s infrastructure issues.
Facial Gold Reinforcement: 28 Years of Experience in the Use of Gold Threads
Authors: Avdeev Alexey E. , Sharobaro Valentin I. , Penaev Arslan A. , Borisenko Anastasia S. , Mitish Elena V. , Bairamova Anna S.
Published: November 2024

Introduction: Gold threads became widely used in esthetic surgery in the early 1990s. Produced in Spain, these threads consisted of a gold thread (5/0) with a diameter of 0.1 mm, composed of 99.99% pure gold, which was combined with a polyglycolic thread. Since then, discussions about their effectiveness have continued, which is the focus of our study. Patients and Methods: Gold thread implantation was performed on 11,062 patients in four clinics in Moscow over the course of 28 years. This study used a comparative analysis of photo collages created for the visual representation of data and changes before and after the procedure, as well as patient satisfaction assessments using the Patient Satisfaction Scale (PSS). We examined the results of 492 patients who underwent gold thread implantation in the face between 1996 and 2024. Of these patients, 86% were women aged 30 to 60 years, 11% were women aged 20 to 30 years, and 3% were men aged 25 to 60 years. Results: One year after thread implantation, the PSS assessment showed a 91% success rate with minimal complications. Patient satisfaction was high, with an average score of 4.8 out of 5 after one month, 4.7 after six months, and 4.6 after one-year post-procedure. Discussion: Gold thread implantation is a minimally invasive procedure that has demonstrated a high level of safety, making it an effective option for facial rejuvenation. Histological studies have shown that gold threads stimulate the production of collagen and elastin and activate angiogenesis, thereby improving skin nourishment and hydration, as well as enhancing the skin tone, elasticity, and turgor. To improve the qualitative characteristics of the skin, it is necessary to work in the subcutaneous layer. Conclusions: Gold thread implantation strengthens the connective tissue framework at the implantation site, thus improving skin nourishment and hydration. Gold threads provide a long-term rejuvenating effect, slowing the ptosis of the soft tissue of the face and neck. Gold thread implantation does not interfere with tissue dissection during surgical interventions or the performance of any cosmetic procedures, including hardware-based treatments.
Median Meld at Transplant Minus 3 Reduces the Mortality of Non-Hepatocellular Carcinoma Patients on the Liver Transplant Waitlist
Authors: Pouramin Panthea , Allen Susan E. , Silburt Joseph L. , Gala-Lopez Boris L.
Published: November 2024

Liver transplants (LTs) are prioritized by mortality risk, which is estimated by MELD scores. Since hepatocellular carcinoma (HCC) patients present with lower MELD scores, they are allocated MELD exception points. Concerns persist that HCC recipients are over-prioritized, resulting in disproportionate waitlist mortality among non-HCC patients. We assessed whether the Median Meld at Transplant minus 3 (MMaT-3) scoring system would balance waitlist mortality and transplantation rates between HCC and non-HCC patients. We reviewed 266 patient charts listed for an LT from 2015 to 2023; 46.2% were listed in the MMaT-3 era. Amongst non-HCC patients, MMaT-3 implementation significantly increased 1-year transplant rate and reduced 1-year waitlist mortality among non-HCC patients (p = 0.003). Pre-MMaT-3 gaps in transplantation (p = 0.004) and waitlist dropout (p = 0.01) were eliminated post-implementation (p > 0.05). Amongst HCC patients, MMaT-3 implementation had no impact on the 1-year transplant rate (p = 0.92) or 1-year waitlist mortality (p = 0.66). Fine-gray proportional hazard multivariable analysis revealed that MMaT-3 significantly reduced waitlist mortality among non-HCC patients (asHR: 0.44, 95% CI [0.23, 0.83], p = 0.01) and limited impact on HCC patients (p = 0.31). MMaT-3 allocation did not significantly alter 2-year post-transplant survival for both populations. We show that the MMaT-3 system decreased the waitlist mortality of non-HCC patients with limited impacts on outcomes for HCC patients listed for an LT.