bluuebunny/crossref_metadata_2025 huggingface dataset:
Induced pluripotent stem cells derived brain endothelial cells from patients suffering from familial form of Alzheimer’s disease display impaired barrier function and cell metabolism
Authors: Patel Ronak , Raut Snehal , Al-Ahmad Abraham
Published: January 2020

Background: Alzheimer’s disease (AD) is the most common most common form of neurodegenerative of neurodegenerative disease. It is an irreversible condition marked by irreversible cognitive loss, commonly attributed by the loss of hippocampal neurons due to the formation of senile plaques and neurofibrillary tangles. Although the sporadic form is the most prevalent, the presence of familial form (involving several genes such as APP, PSEN1 and PSEN2) of the disease is commonly used as a model for understanding the pathophysiology of the disease. The aim of this study is to investigate the effect of mutation on PSEN1 and PSEN2 genes on the BBB function using induced pluripotent stem cells (iPSCs) .
Methods: iPSC lines from patients suffering from familial form of Alzheimer’s diseases and harboring mutations in PSEN1 or PSEN2 were used in this study and compared to a control iPSC line. Cells were differentiated into brain microvascular endothelial cells ( BMECs ) following existing established differentiation protocols. Barrier function was assessed by measuring TEER and fluorescein permeability, drug transporters activity was assessed by uptake assay, glucose uptake and metabolism assessed by cell flux analyzer, mitochondrial potential by JC-1 and lysosomal acidification by acridine orange.
Results: iPSC derived BMECs from the FAD patient presenting a mutation in PSEN1 gene PSEN1-BMECs, but not PSEN2-BMECs, showed impaired barrier function compared to the FAD patient harboring a mutation in PSEN2 and to control group. Such impaired barrier function correlated with poor tight junction complexes and reduced drug efflux pump activity. In addition, both PSEN1 and PSEN2 -BMECs displayed reduced glucose uptake and glycolysis, as well as impaired mitochondrial membrane potential and lysosomal acidification.
Conclusion: Our study reports evidence that PSEN1 and PSEN2 mutations, two genes commonly associated with familial form of Alzheimer’s disease that iPSC -derived BMECs obtained from FAD patients showed impaired barrier properties and BMECs metabolism. In particular, PSEN1 mutation was associated with a more detrimental phenotype than the PSEN2 mutation, as noted by a reduced barrier function, reduced drug efflux pump activity and diminished glucose metabolism . , can impair the development and the maintenance of the BBB, both by an impairement of the barrier function, vesicle trafficking and bioenergetics. Therefore, assessing the contribution of genetic mutations associated with Alzheimer’s disease will allow us to better understand the contribution of the BBB in dementia, but also in other neurodegenerative diseases.
Assessment of Physical Activity of Children and Youth in Podkarpackie Voivodeship
Authors: Krupa Sabina , Ozga Dorota , Stokłosa Patryk
Published: January 2020

The authors have withdrawn this preprint from Research Square
A Pilot Study Comparing Tools for Tracking Implementation Strategies and Treatment Adaptations
Authors: Walsh-Bailey Callie , Palazzo Lorella G. , Jones Salene M. W. , Mettert Kayne D. , Powell Byron J. , Stirman Shannon Wiltsey , Lyon Aaron R. , Rohde Paul , Lewis Cara C
Published: January 2020

Background Tailoring implementation strategies and adapting treatments to better fit the local context may improve their effectiveness. However, there is a dearth of valid, reliable, pragmatic measures that allow for the prospective tracking of strategies and adaptations according to reporting recommendations. This study describes the development and pilot testing of three tools to designed to serve this purpose.Methods Measure development was informed by two systematic reviews of the literature (implementation strategies, treatment adaptation). The three resulting tools vary with respect to the degree of structure (Brainstorming Log = low, Activity Log = moderate, Detailed Tracking Log = high). To prospectively track treatment adaptations and implementation strategies, three stakeholder groups (treatment developer, implementation practitioners, mental health providers) were randomly assigned one tool per week via an anonymous web-based survey for nine weeks and incentivized to participate. Three established implementation outcome measures, the Acceptability of Intervention Measure, Intervention Appropriateness Measure, and Feasibility of Intervention Measure were used to assess the tools. Semi-structured interviews were conducted to gather more nuanced information from stakeholders regarding their perceptions of the tools and the tracking process.Results The three tracking tools demonstrated moderate to good acceptability, appropriateness, and feasibility; the Activity Log was deemed the most feasible of the three tools. Implementation practitioners rated the tools the highest of the three stakeholder groups. The tools took an average of 15 minutes or less to complete.Conclusion This study sought to fill methodological gaps that prevent stakeholders and researchers from discerning which strategies are most important to deploy for promoting implementation and sustainment of evidence-based practices. These tools would allow researchers and practitioners to track whether activities were treatment adaptations or implementation strategies and what barrier(s) each targets. These tools could inform prospective tailoring of implementation strategies and treatment adaptations, which would promote scale out and spread.
Transcriptome analysis following EV-A71 and CV-A16 infection in respiratory epithelial cells
Authors: Song Jie , Li Weiyu , Hu Yajie , Li Hui , Zheng Huiwen , Chen Yanli , Dong Shaozhong , Liu Longding
Published: January 2020

Background: Enterovirus 71 (EV-A71) and coxsackievirus A16 (CV-A16) are the major pathogens responsible for hand, foot and mouth disease (HFMD), but the detailed mechanism caused by the two viruses remains unclear. Methods: In this study, we aimed to adopt transcriptome sequencing technology to investigate changes in the transcriptome profiles after infection with EV-A71 and CV-A16 in human bronchial epithelial (16HBE) cells. Results: Through systematic bioinformatics analysis, we then searched for useful clues regarding the pathogenesis of HFMD. As a result, a total of 111 common differentially expressed genes were present in both the EV-A71- and CV-A16-infected groups. A trend analysis of these 111 genes showed that there were 91 genes displaying the same trend in the EV-A71 and CV-A16 infection groups, including 49 upregulated genes and 42 downregulated genes. These 91 genes were further used to conduct GO, pathway, and coexpression network analyses. It was discovered that the enriched GO terms (such as Histone acetylation and positive regulation of phosphorylation) and pathways (such as glycosylphosphatidylinositol (GPI)-anchor biosynthesis and DNA replication) might be closely associated with the pathogenic mechanism of the two viruses, and key genes (such as TBCK and GPC) might be involved in the progression of HFMD. Finally, we randomly selected 10 differentially expressed genes for qRT-PCR to validate the transcriptome sequencing data. The experimental qRT-PCR results were roughly in agreement with the results of transcriptome sequencing. Conclusions: Collectively, our results provide clues for the pathogenesis of HFMD induced by EV-A71 and CV-A16.
Traditional Practices Influencing the Use of Maternal Health Services in Indonesia
Authors: Aryastami Ni Ketut , Mubasyiroh Rofingatul
Published: January 2020

Background Although infrequent, use of traditional birth attendants (TBA) for delivery still exists in Indonesia. The maternal mortality ratio (MMR) at 305 deaths per 100,000 live births in Indonesia is higher than that of the Southeast Asian region (240), and continued use of TBA is suspected to be related to this high MMR. The objective of this analysis is to study the influence of traditional practices, family structure, and TBA density on the use of maternal health services in Indonesia.Methods Secondary data analysis involved data from two national surveys— Riset Kesehatan Dasar (Riskesdas) 2010 and the Village Potential Survey 2008. The sample included 14,798 mothers whose last delivery was between January 2005 and August 2010. The independent variables were the use of traditional practices, type of family structure, and density of TBA. The dependent variables were utilization of maternal healthcare, including receiving antenatal care (ANC) and having a facility-based delivery. Multivariate logistic regression was conducted for the analysis by controlling all the covariates. Results Disregarding the costs for delivery, traditional practices and high TBA density significantly influenced mother’s access to maternal health utilization with a negative direction which is conversely to living in extended family structure. Mothers in villages with a high TBA density had significantly lower odds of receiving complete care and facility-based delivery compared to mothers in villages with low TBA density. Moreover, mothers who lived in an extended family had higher odds of using maternal health services. Conclusions Traditional practices significantly influenced the ideal utilization of maternal health care in Indonesia. Higher TBA density in the villages significantly decreased mother’s odds of using ANC, skilled birth attendance (SBA), and the complete of maternal health services. Maternal care utilization was positively influenced by living with extended family.
Postural control learning dynamics in Parkinson’s disease: early ‎improvement with plateau in stability, and continuous progression in ‎flexibility and mobility
Authors: Rahmati Zahra , Behzadipour Saeed , Schouten Alfred C. , Taghizadeh Ghorban , Firoozbakhsh Keikhosrow
Published: January 2020

Background: Balance training improves postural control in Parkinson’s disease (PD). However, a systematic approach for the development of individualized, optimal training programs is still lacking, as the learning dynamics of the postural control in PD, over a training program are poorly understood.Methods: We explored the learning dynamics of the postural control in PD, during a balance-training program, in terms of the clinical, posturographic, and model-based measures. Twenty patients with PD participated in a balance-training program, 3 days a week, for 6 weeks. Clinical tests assessed functional balance and mobility pre-training, mid-training, and post-training. Center-of-pressure (COP) was recorded at four time-points during the training (pre-, week 2, week 4, and post-training). COP was used to calculate the sway measures and to identify the parameters of a patient-specific postural control model, at each time-point (stability and flexibility degree). The posturographic and model-based measures constituted the two sets of stability- and flexibility-related measures.Results: Mobility- and flexibility-related measures showed a continuous improvement during the balance-training program. In particular, mobility improved at mid-training and continued to improve to the end of the training, whereas flexibility-related measures reached significance only at the end. The progression in the balance- and stability-related measures was characterized by early improvements over the first three to four weeks of training, and reached a plateau for the rest of the training. Conclusions: The progression in balance and postural stability is achieved earlier and susceptible to plateau out, while mobility and flexibility continues to improve during balance training.
The Impact of Fluid Resuscitation on Hemodynamic of Hemorrhagic Shock: An Animal Model Experimental Study
Authors: Pudjiadi Antonius Hocky , Firmansyah Agus , Soeyono Gunanti , Bardosono Saptawati , Jusman Sri Widia , Siagian Minarma , Lubis Munar
Published: January 2020

Background: To investigate the effect of fluid resuscitation on glycocalyx shedding, and extravascular lung water index (ELWI), mean arterial pressure (MAP) and oxygen delivery (DO2) changes.Methods: Male domestic piglets (Sus scrofa) 6-10 weeks old anesthetized and bled until mean arterial pressure drop to 20% of baseline and resuscitated with normal saline as much as blood drowned, followed with 40 mL/kg of normal saline after 30 minutes. Cardiac index (CI), ELWI, systemic vascular resistance index (SVRI), MAP, atrial natriuretic peptide (ANP) and syndecan-1 were measured before and after each fluid resuscitations. Results: Serum ANP was increased after normal volume fluid resuscitation (p= 0.043) and return its baseline value after hypervolemia fluid resuscitation. Serum Syndecan-1 levels did not increase. A small increase in ELWI only found 60 minutes after fluid resuscitation (p= 0.021). SVRI undergo a gradual decrease, until the lowest value at hypervolemia volume resuscitation. There was no difference between the MAP of the two groups (p= 0.105). Hemoglobin concentration significantly decreased from normal to hypervolemia volume resuscitation (p= 0.009). Oxygen delivery in hypervolemia resuscitation is higher than in normal volume resuscitation (p= 0.012), due to a significant increase in CI at hypervolemia volume resuscitation (p<0.001).Conclusions: Hypervolemia fluid resuscitation in the animal hemorrhage model is not induced glycocalyx shedding. Small increase ELWI was found in 60 minutes after fluid resuscitation. DO2 is maintained by increasing CI in spite of decreasing hemoglobin level due to hemodilution. Increasing CI is balanced by reducing SVRI to sustain stable MAP.
Investigation of de novo mutations in a schizophrenia case-parent trio by induced pluripotent stem cell based in vitro disease-modeling: Convergence of schizophrenia and autism-related cellular phenotypes
Authors: Hathy Edit , Varga Nóra , Erdei Zsuzsa , Szabó Eszter , Tordai Csongor , Czehlár Boróka , Baradits Máté , Jezsó Bálint , Koller Júlia , Nagy László , Molnár Mária Judit , Nemoda Zsófia , Homolya László , Apáti Ágota , Réthelyi János M
Published: January 2020

Background: De novo mutations (DNMs) have been implicated in the etiology of schizophrenia, a chronic debilitating psychiatric disorder characterized by hallucinations, delusions, cognitive dysfunction and poor community functioning. Several DNMs have been shown by examining schizophrenia cases and their unaffected parents, however in most cases the biological significance of these mutations remains elusive. To overcome this limitation we have developed an approach of using somatic cell reprogramming to generate induced pluripotent stem cell (iPSC) lines from each member of a schizophrenia case-parent trio, in order to investigate the effects of DNMs in cellular progenies of interest, particularly in dentate gyrus neuronal progenitors. Methods: We identified a male schizophrenia patient characterized by early disease onset and negative symptoms, who is a carrier of 3 non-synonymous DNMs in genes LRRC7, KHSRP, and KIR2DL1. iPSC lines were generated from his and his parents’ peripheral blood mononuclear cells using Sendai virus-based reprogramming. After characterization the iPSCs were differentiated into neuronal progenitor cells (NPCs) and hippocampal dentate gyrus granule cells for the investigation of various cellular phenotypes. We used RNASeq to investigate and transcriptomic differences, calcium (Ca 2+ ) imaging, cell proliferation, migration, oxidative stress, and mitochondrial assays to characterize the investigated NPC lines. Results: NPCs derived from the schizophrenia patient exhibited transcriptomic differences related to Wnt-signaling, axonal guidance and synapse formation, and decreased Ca 2+ reactivity to glutamate. Moreover we could observe increased cellular proliferation and migration, accelerated neurite outgrowth rates, and alterations in mitochondrial quantity and morphology. Conclusions: The approach of reprograming case-parent trios represents a possibility of investigating disease-causing mutations and comparing cell lines with reduced variation in genetic background. Our results are indicative of an overlap between schizophrenia and autism-related phenotypes in the investigated family.
Renal dysfunction decreases specificity and overturns cutoff value of serum CC16 while prohibiting 7-day mortality prediction in diagnosing acute respiratory distress syndrome in intensive care unit.
Authors: Lin Jinle , Tao Wuyuan , Wei Jian , Jian Wu , Zhang Wenwu , Ye Jianbing , Fu Xuan , Dou Qingli , Wang Lijun , Tian Fang
Published: January 2020

Background: A contradictory tendency between occurrence of acute respiratory distress syndrome (ARDS) and serum club cell protein 16 (CC16) level, However, renal dysfunction (RD) separately raised serum CC16 in our current observation. The purpose of this study was to find the limitation caused by renal dysfunction in the diagnostic performance of CC16 on ARDS in intensive care unit (ICU) patients. Method: We measured serum CC16 in 479 ICU patients. Patients were divided into six subgroups: control, acute kidney injury (AKI), chronic kidney dysfunction (CKD), ARDS, ARDS+AKI, and ARDS+CKD. The cutoff value, sensitivity and specificity of serum CC16 were assessed by receiver operating characteristic curves. Result: Serum CC16 increased among the ARDS group when compared to the control group, which helps identify ARDS and predicts the outcome in patients with normal renal function. However, level of serum CC16 was similar among ARDS+AKI, ARDS+CKD, AIK and CKD groups. Consequently, when compare to AKI and CKD, specificity for diagnosing whether ARDS or ARDS with renal failure decreased from 86.62% to 2.82% or 81.70% to 2.12%. Consistently, a cutoff value of 11.57 ng/mL was overturned from previously at 32.77 ng/mL or 33.72 ng/mL. Moreover, its predictive value for mortality is prohibited before 7 day but works after 28 day. Conclusion: Renal dysfunction limits the specificity, cutoff point, and predictive value at 7-day mortality of CC16 in diagnosing ARDS among ICU patients.
A multifactorial evaluation for the effects of air pollution and meteorologic factors on asthma exacerbation
Authors: Yu Hong-Ren , Lin Chun-Hung Richard , Tsai Jui-Hsiu , Hsieh Yun-Ting , Tsai Ti-An , Tsai Chang-Ku , Lee Yi-Chen , Liu Ta-Yu , Tsai Chih-Min , Chen Chih-Cheng , Chang Chih-Hao , Hsu Te-Yao , Niu Chen-Kuang
Published: January 2020

Background: Study identify individual effect of single pollutants on asthma acute exacerbation (AE) may overlook the health effect of the mixture overall. In real world, dynamic changes of air pollutants and meteorologic factors coexist simultaneously. A comprehensive study was carried out to examine the influence of air pollution and meteorologic factors on asthma AE. Materials and methods: The asthma AE data from emergency room visits were collected from the Taiwan National Health Insurance Research Database from 2005 to 2013. Complete monitoring data for the air pollutants (sulfur dioxide, SO2; nitrogen dioxide, NO2; ozone, O3; carbon monoxide, CO; PM2.5; and PM10) and meteorologic factors (relative humidity, rainfall, and daily average temperature) were collected from Environmental Protection Agency monitoring stations. A bi-directional case-crossover analysis was used to investigate the effects of air pollution and meteorological factors on asthma exacerbation. Result: Along age group divisions, a 1 ℃ temperature increase was a protective factor for asthma ER visits, with OR = 0.981 (95% CI 0.971–0.991) and 0.985 (95% CI, 0.975–0.994) for pediatric and young adult patients, respectively. Each 1 mg/m3 increase in the 48-h averages of PM2.5 was associated with asthma ER visit for patients older than 65 years of age (OR = 1.008 (95% CI, 1.003–1.014). Conclusion: In Taiwan, asthma AE is closely related to low temperature and certain air pollution. Patients can take appropriate protective strategies to minimize risk of asthma AE related to air pollution/meteorological factors according to relevant gender and age.
Comprehensive Analysis of Evolutionary, Characterization and Expression for Sugar Transporter Family Genes in Nelumbo nucifera 
Authors: Wu Peng , Zhang Yongyan , Li Liangjun
Published: January 2020

BackgroundThe sugar transporter , an important class of transporter for sugars function, play regulators of many processes associated with growth, maturation and senescence processes in plant.ResultsIn this study, a total of 35NNUSTs were identified in the Nelumbo nucifera genome. Furthermore, all NNUSTs genes identified were grouped according to conserved domains and phylogenetic analysis. Additionally, we identified 316 ST genes in other10 representative plants, and performed a comparative analysis with Nelumbo nucifera, including evolutionary trajectory, gene duplication, and expression pattern. A large-scale analysis across plants and alga suggested that the ST family could have been originated from STP and Glct, expanding to form STP and SFP by dispersed duplication. Finally, quantitative real-time polymerase chain reaction and cis-elements analysis showed that the NNUSTs were response to abiotic stresses. ConclusionsThis provides useful resources when exploring the molecular evolution and mechanisms of NNUSTs in plants.
Construction of a High-Density Genetic Map and Mapping of Major Flowering Time QTLs in Rapeseed (Brassica napus L.) Based on Whole-Genome Re-sequencing
Authors: Li Xin , Du Dezhi , Ma Qianru , Li Linfang , Zhao Zhi , Tang Guoyong , Liu Haidong , Xiao Lu
Published: January 2020

BackgroundFlowering time is an important trait impacting the performance of Brassica napus L. cultivars, but the interaction of all the loci controlling this trait in spring-type oilseed rape is not fully understood.ResultsIn this study, we conducted a genome-wide quantitative trait locus (QTL) analysis of a recombinant inbred line (RIL) population composed of 215 lines and constructed a high-density linkage map with 4523 recombination bin markers and of 92,627 single-nucleotide polymorphisms (SNPs). After phenotypic measurements across four different fields environments were collected for 2 years, at least 27 flowering time QTLs were identified; these were distributed mainly on linkage groups (LGs) A02 (11 QTLs), A10 (1 QTL), C02 (12 QTLs), C05 (2 QTLs) and C08 (1 QTL). Of these QTLs, five major QTLs for flowering time were detected, and three of them, sqFT-A02-3, sqFT-A02-4, and sqFT-A10-1, were identified only in winter ecological conditions; thus, these QTLs were environment specific. The QTL cqFT-C02-2 was detected in all 3 spring environments, showing strong spring environmental specificity. The major QTL cqFT-C02-3, which is an environmentally stable QTL, was detected in all 4 environments and was expressed both in spring and winter ecological conditions. The winter ecological condition-specific major QTL sqFT-A02-4 was delimited to a region of approximately 216 kb, between 6.302 Mb and 6.518 Mb on A02. In this region, the BnaA02g12130D/GSBRNA2T00090951001 gene was identified as a homologue of the Arabidopsis thaliana FLOWERING LOCUS T (FT) gene by a BLASTn analysis of the candidate region.ConclusionsFive major novel QTLs for flowering time were discovered in spring Brassica napus: 1 environmentally stable QTL and 4 environment-specific QTLs. The major QTL sqFT-A02-4 was delimited to a candidate region of approximately 216 kb, and BnaA02g12130D was determined to be a potential gene
Prediction key genes and the potential molecular mechanism for early recurrent pregnancy loss by Multi-Omics analysis
Authors: Huang Le , Wei Changqiang , Zhang Zhao , Cen Jiao , Zhu Zhiwei , Deng Lingjie , Pang Lihong
Published: January 2020

BackgroundThe potential mechanism of early recurrent pregnancy loss (ERPL) has not been fully elucidated, a multi-omics analysis can help us find key genes.ResultsWe download data from Gene Expression Omnibus (GEO), 90 hypermethylation-down regulated genes and 49 hypomethylation-up regulated genes were identified by intersection. Compared with the normal early pregnancy group, the expression of ERCC2 and MLH1 was lower in ERPL group, but the expression of PLEK and FOS was higher, and the expression of MLH1 was statistically significant (p<0.05). Compared with the anembryonic (empty sac) miscarriage group, the expression of MLH1, PLEK, FOS decreased, and that of ERCC2 increased in the embryonic miscarriage group. TF-MDEGs networks predicted SP1, POLR2A, YY1, CREM and CREB1 were involved in methylation regulation of DNA promoter with MDEGs. Among them, YY1, FOXP3 and p53 may be related to the mechanism of MLH1 in ERPL.ConclusionsOur study identified possible aberrant MDEGs, and TF- MDEGs regulatory networks may be related to its mechanism. MLH1 may play an important role in early embryonic development.
Prioritising key motivators and challenges influencing informal carers’ decisions for participating in randomised trials: a Study Within A Trial (SWAT-55)
Authors: Smith Valerie , Corry Margarita , Devane Declan , Treweek Shaun , Hunter Andrew , Grylka-Baeschlin Susanne , Hannon Kathleen
Published: January 2020

Background: Family members, or others, often assume the role of informal (unpaid) carers of people with chronic illnesses. Care-giving, however, can impact profoundly on the quality of life of caregivers and can cause carer worry, stress and guilt. Implementing interventions that positively affect the lives of carers is important, however, carers as a group are often difficult to reach. We embedded a study within a pilot-feasibility study of a mindfulness based intervention for carers of people with chronic illness to determine the key motivators and challenges influencing informal carers’ decisions for participating in a trial, and to prioritise these to inform future trial design and conduct. Methods: We conducted a Study Within A Trial using a multi-method approach involving interviews with participants from the ‘host trial’ and data from systematic reviews to develop a survey that was distributed to informal carers across Ireland. The survey consisted of 28 motivator and 17 challenge statements. Participants rated how important they thought each statement was when deciding to take part in a trial on a 5-point Likert Scale (1-very unimportant to 5-very important). Mean scores and standard deviations were calculated for each statement and arranged in descending order to provide the final priority lists. Results: Thirty-six carers responded to the survey. Helping to create awareness about carers was the top ranked motivator, followed by four study design statements related to the time at which the study occurs, the study location, format of delivery and venue. The least important motivator related to how carers were invited to take part in a study. Difficulties in planning due to the caring role emerged as the most important challenge for carers, followed by being unable to leave the care recipient on his/her own. Conclusions: Insight into decision-making for research participation will assist trial developers tailor trial processes for informal caregiver populations. We recommend that trialists consider these motivators and challenges when designing future trials involving informal caregivers so as to enhance trial feasibility and success.
Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicated with Different Serum Albumin Levels
Authors: Han Shuwen , Wu Wei , Da Miao , Zhou Qing , Xu Jiamin , Zhuang Jing , Liu Jin , Yang Xi
Published: January 2020

Background: Patients with colorectal cancer (CRC) are at risk of malnutrition. Gut microbes and microbial metabolites are involved in the initiation and development of CRC.Purpose:　To investigate serum protein levels in CRC patients and explore the role of gut microbes and microbial metabolites in CRCs complicated with different serum albumin levels.Methods: Overall, 398 CRC patients and same number of healthy volunteers in Huzhou Central Hospital from January 2016 to December 2018 were recruited to compare serum protein levels. The serological indicators were detected by Abbott Automatic Biochemical Analyzer(HCHL-YQ-SH-01). A total of 30 and 56 stool samples from CRC patients were used to detect intestinal microbes and microbial metabolites, respectively. Bacterial 16S V3-V4 and fungal ITS ribosomal DNA genes were sequenced and gas chromatography/mass spectrometry (GC/MS) was performed to detect microbial metabolites. Results: Some serum protein-related indicators in the CRC group were lower than those in the control group (p＜0.05). The total protein and albumin levels in colon cancer patients were lower than those in rectal cancer patients (p＜0.05). The higher abundance of Sutterella is correlated with lower serum albumin level in CRCs. There were statistically significant differences in the abundance of fungi including Agaricomycetes， Simplicillium, Sclerotiniaceae, and Exophiala among patients with different serum albumin levels. Multiple gut bacteria and fungi are closely related to serum albumin levels.We found some characteristic microbial metabolites in CRCs complicated with different serum protein levels.Conclusions: The different serum albumin levels were associated with the gut microbes and microbial metabolites in CRCs. It may provide novel ideas for basic research and clinical application.
Histological and immunohistochemical evaluation of epithelial and mesenchymal tumors of psittacines
Authors: BAMAC OZGE ERDOGAN , S. SECKIN ARUN
Published: January 2020

This study describes histological and immunohistochemical evaluation of 50 epithelial and mesenchymal tumors of psittacines that were found in the archives of the pathology departments of veterinary faculties at Utrecht University and Istanbul University Cerrahpasa. In each case, cellular morphological features, tumoral pattern and mitotic activity were evaluated histologically, and immunohistochemistry was performed by the streptavidin-biotin immunoperoxidase method using a panel of commercially available antibodies raised against pan-cytokeratin, vimentin, muscle-specific actin, desmin, smooth muscle actin and Factor VIII/von Willebrand Factor. The histological evaluation revealed that 30 epithelial tumors were squamous cell carcinoma (n: 20), renal adenocarcinoma (n: 3), basal cell carcinoma (n: 2), abdominal carcinoma (n: 2), uropygial gland carcinoma (n: 1), cloacal carcinoma (n: 1) and cholangiocarcinoma (n: 1). Out of the 50 tumors, 13 had a mesenchymal origin and were diagnosed as fibrosarcoma (n: 4), hemangioma (n: 3), hemangiosarcoma (n: 4) and leiomyosarcoma (n: 2). Histological diagnosis could not be made for 7 tumors, and they were evaluated as undifferentiated malignant tumors (UDMT). Two tumors diagnosed as fibrosarcoma and three tumors diagnosed as UDMT showed strong SMA staining and were re-diagnosed as leiomyosarcoma. In view of the IHC findings, two other UDMTs were re-diagnosed as fibrosarcoma and anaplastic carcinoma. Although only a limited number of antibodies were used in the control panel, they were useful in establishing a definite diagnosis in some cases
BURSA VAKIF KÜLTÜRÜ MÜZESİ’NDE BULUNAN KUMAŞ ÜZERİNE İŞLEMELİ HAT LEVHALARI
Authors: DAĞDELER Coşkun , YAVAŞ Doğan
Published: January 2020

Türk işleme sanatı, Osmanlı döneminde en parlak
devrini yaşamış ve bir saray sanatı olarak zirve noktasına ulaşmıştır. Kültür
ve sanatta özgün olan Türkler, İslâm kültür ve medeniyeti doğrultusunda Arap
yazısına bağlı olarak ortaya çıkmış güzel sanatların bir dalı olan hat sanatını
en üst düzeye çıkarmışlardır. Bu çalışmada, Bursa Büyükşehir Belediyesi Kültür
ve Sanat Dairesi Başkanlığı Müzeler Şube Müdürlüğü bünyesinde hizmet veren
Bursa Vakıf Kültürü Müzesi’nde bulunan kumaş üzerine işlemeli hat örnekleri
incelenmiştir. Bu incelenen eserler, Bursa Emir Sultan Camii ve Bursa Muradiye
Camii’ne bağışlanmış ve 31.03.2016 tarih E.58968 sayılı protokolün 14.04.2016
tarihinde onaylanmasıyla Vakıflar Genel Müdürlüğü tarafından Bursa Vakıf
Kültürü Müzesi’nde sergilenmesi için verilen eserlerden oluşmaktadır. Muhtelif renklerde
kumaş ve ipliklerle, çeşitli işleme teknikleriyle işlenen her bir eser için
fotoğraflar çekilmiş ve bilgi fişleri oluşturulmuştur. Hat levhalarının
okunuşları ve açıklamaları, dönemleri ve hattatları, ebatları, bezemelerinde
kullanılan işleme teknikleri ve süsleme özellikleri gibi bilgiler envanter
kayıtlarına dayanarak incelenmiştir.
BİLİNÇLİ FARKINDALIK VE BİLİŞSEL ESNEKLİĞİN PROBLEMLİ AKILLI TELEFON KULLANIMI ÜZERİNDEKİ ETKİSİ: ÖZ-KONTROLÜN ARACI ROLÜ
Authors: KAYMAZ Ekrem , ŞAKİROĞLU Mehmet
Published: January 2020

Bu çalışmanın temel amacı, üniversite öğrencileri örnekleminde bilinçli farkındalık ve bilişsel esnekliğin problemli akıllı telefon kullanımı ile ilişkisinde öz-kontrolün aracı rolünü ortaya koymaktır. Örneklem, üniversitede öğrenim gören 171’i (%57) kız, 125’i (%43) erkek olmak üzere toplam 296 üniversite öğrencisinden oluşmuştur. Araştırmada veri toplamak amacıyla “Kişisel Bilgi Formu”, “Kısa Öz-kontrol Ölçeği”, “Bilinçli Farkındalık Ölçeği”, “Akıllı Telefon Bağımlılığı Ölçeği” ve “Bilişsel Esneklik Envanteri” kullanılmıştır. Araştırmanın bulgularına göre, öz-kontrolün, bilişsel esneklik ve problemli akıllı telefon kullanımı arasında tam aracı rol gösterdiği; bilinçli farkındalık ve problemli akıllı telefon kullanımı arasında kısmi aracı rol gösterdiği bulunmuştur. Ayrıca, öz-kontrolün, bilişsel esnekliğin alt boyutlarından alternatif ve problemli akıllı telefon kullanımı arasında tam aracı rol gösterdiği; kontrol ve problemli akıllı telefon kullanımı arasında ise kısmi aracı rol gösterdiği bulunmuştur. Bulgular ilgili literatür çerçevesinde tartışılmıştır.
The Post-Discharge Growth of Very Low Birth Weight Preterm Infants
Authors: Ozdemir Tuba , Akcan Abdullah Baris , Turkmen Munevver Kaynak
Published: January 2020

OBJECTIVE: In the present study, we investigate the growth characteristics of very low birth weight premature infants of up to two years corrected age, considering the factors affecting growth and catch-up growth time.STUDY DESIGN: The demographic data, clinical features, and comorbidities of 77 preterm infants with birth weights of less than or equal to 1.500 g were examined, the infants’ growth statuses in the 40th gestational week (gw) and at 6, 12, 18 and 24 months the corrected age, including their weight, height and head circumference, were evaluated.RESULTS: The findings revealed that very low birth weight infants should be closely monitored either during their stay in the Neonatal Intensive Care Unit, or for up to 6 months corrected age, paying particular attention to growth data, and the appropriate supportive treatment should be administered. The applied support process is influential on the future somatic growth of preterm infants. It was noted in the study that bronchopulmonary dysplasia, proven sepsis, respiratory distress syndrome, steroid treatment for more than three days, patent ductus arteriosus, and ibuprofen treatment seemed to affect somatic growth negatively.CONCLUSION: Small for gestational age newborns were found to catch up with appropriate for gestational age newborns at 2 years corrected age in terms of growth, although the percentage of catch-up growth during follow-up at the 40thgw, and at the 6th, 12th and 18th months was lower than that of appropriate for gestational age newborns.
History of the Creation of the Tobolsk Diocese in the Official Correspondence of the 17th century
Authors: Shcherbich Sophia N.
Published: January 2020

This article studies the correspondence of the 17th century on the creation of the Tobolsk diocese in Siberia. The documentary complex has been preserved in the book of the Siberian Prikaz, published in 1994. It consists of two types of documents (letters by tsar, patriarch and archbishop, as well as the replies of the voivodes), which allow determining the mechanism for making managerial decisions and the circumstances of their implementation.
Based on the historical and anthropological approach, the author considers the process of creating diocese from the perspective of its participants, focuses on interpersonal interaction, a behavioral culture that has been consolidated in documents. The embodiment of the tsarist plan into reality is a challenge that each of the participants overcomes independently, setting priorities between state interests and personal ones. The dating of the letters allows identifying three stages of documenting the tsar’s decisions on the creation of the Tobolsk bishopric, which allowed revealing the specifics of the communication channels of the center and periphery in the Russian state in the 17th century. Based on the sources, the author tries to answer question: what could be the reason that prompted the first archbishop of Cyprian to agree with the tsar’s decision and to head the new diocese created (according to the documents) in September 1620?
Analyzing the correspondence, the author draws attention to the difficulties that the head of the new diocese faced on his way to Siberia and upon arriving in Tobolsk, as well as the details of the ceremonial that the Tobolsk voivodes had to observe during the construction of the bishop’s house and the organization of the meeting of the Siberian archbishop.
The Inner World of Homo in the light of Cultural Codes Represented in Russian and Kazakh Literary discourse
Authors: Rumyantseva Marina V.
Published: January 2020

The last several decades saw the growing relevance of studying culture through language because latter provides researchers with information about the world and a person’s place within it. All these different manifestations of human culture, reflected and fixed in the language, are studied by linguoculturology.
This article explores the linguocultural manifestations of different yet interrelated ethnic groups — Russian and Kazakh. The purpose of this study was to describe the inner world of a person, his or her feelings and emotions in the process of linguocultural analysis of cultural codes by which this world is represented. The study was performed on the material of fictitious texts of Russian and Kazakh authors.
The author defines the notion “cultural code” and analyzes figurative (metaphoric) com­parisons, which, along with metaphor and metonymy, are one of the ways to create a secondary world. The components of the figurative comparison belong to different orders, while the ability of human consciousness to correlate phenomena from different spheres of the surrounding world lies at the heart of the system of cultural codes.
The results show that the main cultural codes arising in the Russian and Kazakh fiction in describing the inner world of homo, belong to natural and biomorphic codes. The author concludes that the presence of natural tokens and biomorphic cultural tokens in comparisons of human feelings and emotions reflect the archetypal ideas of people about nature. This means that in the consciousness of a modern person there is still an inextricable link between the substances “the inner world of human” and “nature”. The inner world is an integral part of the nature, which is reflected in the language and culture.
Understanding the Molecular Origins of Pulmonary Hypertension: Role of MicroRNAs
Authors: Bonnet Sebastien
Published: January 2020

Pulmonary arterial hypertension (PAH) is a disease of the pulmonary vasculature, defined by an elevated pulmonary vascular resistance, leading to right heart failure and premature death. The cause remains unknown and available treatments are limited. PAH is characterized by enhanced pulmonary artery smooth muscle cell (PASMC) and pulmonary artery endothelial cell (PAEC) proliferation and suppressed apoptosis within the pulmonary artery wall. It has been shown that this phenotype is associated with mitochondrial hyperpolarization and enhanced glycolysis over glucose oxidation (Warburg effect), which are sustained over time by the activation of the transcription factors HIF-1 and NFAT. Nonetheless, the mechanisms accounting for these abnormalities remain unknown. A common feature to all vascular remodeling processes is that in early stages of the disease, a significant increase in oxidative stress and inflammatory processes are observed, causing irreversible DNA damage and cell death.
LOW ENERGY BACTERIA PRESERVATION OF EXTREMELY HALOPHILIC ARCHAEA HALOFERAX LUCENTENSE AND HALOFERAX CHUDINOVII IMMOBILIZED USING NATURAL ZEOLITE
Authors: Malik Rizal Awaludin , Nilawati Nilawati , Handayani Novarina Irnaning , Rame Rame , Djayanti Silvy , Pratiwi Ningsih Ika , Setianingsih Nanik Indah , Nasuka Nasuka
Published: January 2020

The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research was the type of the carrier which was raw zeolite, 110oC and 300oC heat-activated zeolite carrier, parameters measured in this study was physical and chemical of zeolite such as chemical content, Si/Al ratio, surface area and pore volume, and biochemical assay, bacterial cells numbers after immobilization and bacterial cells after preservation as bacterial response to the immobilization and preservation. Heat activation was significantly affecting the chemical composition, carrier surface area, and pore volume. Highest surface area, pore volume, and Si/Al ratio were obtained in 110oC pretreated zeolite followed by 300oC pretreated zeolite. The bacterial cells obtained after immobilization process was 1,8x107 cfu/g, 3,0 x 107 cfu/g, and 2,1x107 for raw zeolite, 110oC pretreated zeolite and 300oC zeolite respectively. After 4 months preservation, the slight reduction of the bacterial cells was observed. Immobilization halophilic archaeae using zeolite as carrier was proven as low cost and effective preservation method due to relatively simple process and unspecific preservation temperature requirements.
The Effect of Service Quality and Facilities against Satisfaction of Patients at Dental and Mouth Hospital Gusti Hasan Aman on Banjarmasin
Authors: Daud Ibrahim , - Dhelia
Published: January 2020

Human needs are unlimited and continue to evolve with the times, in this modern era people are not only focused on the hierarchical needs (hierarchy needs) but also the need for health services. Health services can be obtained by the community through hospitals, polyclinics, community health centers (puskesmas) and family doctors. This study aims to analyze the effect of service quality (X1) and facilities (X2) on patient satisfaction (Y) in RSGM-GHA. The study was conducted on 4,988 patients who came for treatment at RSGM-GHA and 100 patients were taken as samples. The analytical method used is multiple linear regression statistical analysis with the help of the SPSS program. The results showed that the variable services quality and facilities provided by the Gt. Hasan Aman to patient gives satisfaction with t test value: service quality (X1) of 0.679 with a significant 0.499> 0.05 and t-count is smaller than t table 0.679 <1.988 which means that there is no influence. While facilities (X2) of 13,242> 1,988 with a significant level of 0.00 showed a significant effect on patient satisfaction. F-test or simultaneous test shows that together service quality variables (X1) and Facility variables (X2) influence with a value of 215,812 significance level of 0,000 and R square value of 0.817 which means that both variables are very strong influence by 81.7 percent.
The Over-Searching Accidents Causative Factors in Ghana: The Role of Policyholders Education Levels
Authors: Jacob Azaare , Wu Zhao , Ahia Bright Nana Kwame , Amankwah Edward
Published: January 2020

Available statistics indicates that about 90% of all claims or accident in Ghana is caused by human behavior. Therefore, policyholders’ errors are categorized depending on the severity and extend of casualties caused as a result of misinterpretation of road traffic control devices based on their education levels. Hence, in order to ascertain all the possible causes within the human element to reduce the increasing trend of yearly claims, this study report on the influence of education levels on accident/claims frequency and severity drawing upon a purposive sample of 203 policyholders who have experienced at least one accident in a year using structural equation modeling (SEM). The findings from our regression weights gave enough evidence to reject most of our hypotheses with few ones being supported. This study provides enough evidence that education generally to perspective policyholders influence accidents/claims occurrence. However, in terms of education levels of policyholders, we did not have enough evidence in support of any of these levels either causing or reducing claims/accident frequency. Besides accident/claim frequency, we extended our regression analysis on claim severity and also included some well know auto insurance rating factors to ascertain their impacts on accident frequency. Consequently, it was revealed that most of the severe claims or accidents that results into deaths and serious injuries on yearly basis are caused by policyholders or drivers with medium level of education in Ghana with its frequency driving mostly by rating factors such as the vehicle’s age, cubic capacity, mileage, etc.
Letrozole before TESE in Non-Obstructive Azoospermia, Does It Affect Sperm Retrieval Rate, A Retrospective Study
Authors: Al Edwan Ghazi M. , Mansi Hammam H. , Al-Shalaby Ahmed Zuhier , Al-Tell Tareq Laith , Atta Omar Nabeeh M. , Al-Dmour Mohammad Hani , Yusof Nur Amalina , Fuzai Tuan Nur Aisyah Tuan , Mohamad Nor Nor Amalina , Abd aziz Nurul Fadzilah
Published: January 2020

Objective: This study was designed to evaluate the effect of letrozole 2.5 mg, an aromatase inhibitor, on the sperm retrieval rate (SRR) by the testicular sperm extraction (TESE) procedures that was done for the treatment of males with non-obstructive azoospermia (NOA).Materials and methods: Data was collected retrospectively from males diagnosed with non-obstructive azoospermia who underwent TESE procedure in the duration between May 2010 until June, 2018. The collected data includes the age of the patient, body mass index (BMI), testicular volume, hormonal profile (FSH LH, prolactin, testosterone), and the use of letrozole preoperatively. Logistic regression was done to address the association of these parameters to the sperm’s retrieval rate.Results: The study screaned 145 patients. Eighty patients fit the inclusion criteria and thus they were statistically analyzed. The use of letrozole was associated with negative TESE outcome (p=0.006), odd (0.154) CI 0.04-0.579. The other factors had no significant correlation to the TESE results.Conclusion: The evidence in this study showed an adverse effect of letrozole use on TESE results of those with high FSH.