bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparison of Differential Gene Expression in Response to the Onset of Water Stress Between Three Hybrid Brachiaria Genotypes
Authors: Jones Charlotte , De Vega Jose , Worthington Margaret , Thomas Ann , Gasior Dagmara , Harper John , Doonan John , Fu Yuan , Bosch Maurice , Corke Fiona , Arango Jacobo , Cardoso Juan Andres , de la Cruz Jimenez Juan , Armstead Ian , Fernandez-Fuentes Narcis
Published: March 2021

Brachiaria (Trin.) Griseb. (syn. Urochloa P. Beauv.) is a C4 grass genus belonging to the Panicoideae. Native to Africa, these grasses are now widely grown as forages in tropical areas worldwide and are the subject of intensive breeding, particularly in South America. Tolerance to abiotic stresses such as aluminum and drought are major breeding objectives. In this study, we present the transcriptomic profiling of leaves and roots of three Brachiaria interspecific hybrid genotypes with the onset of water stress, Br12/3659-17 (gt-17), Br12/2360-9 (gt-9), and Br12/3868-18 (gt-18), previously characterized as having good, intermediate and poor tolerance to drought, respectively, in germplasm evaluation programs. RNA was extracted from leaf and root tissue of plants at estimated growing medium water contents (EWC) of 35, 15, and 5%. Differentially expressed genes (DEGs) were compared between different EWCs, 35/15, 15/5, and 35/5 using DESeq2. Overall, the proportions of DEGs enriched in all three genotypes varied in a genotype-dependent manner in relation to EWC comparison, with intermediate and sensitive gt-9 and gt-18 being more similar to each other than to drought tolerant gt-17. More specifically, GO terms relating to carbohydrate and cell wall metabolism in the leaves were enriched by up-regulated DEGs in gt-9 and gt-18, but by down-regulated DEGs in gt-17. Across all genotypes, analysis of DEG enzyme activities indicated an excess of down-regulated putative apoplastic peroxidases in the roots as water stress increased. This suggests that changes in root cell-wall architecture may be an important component of the response to water stress in Brachiaria.
Mood Disorder in Cancer Patients Undergoing Radiotherapy During the COVID-19 Outbreak
Authors: Nardone Valerio , Reginelli Alfonso , Vinciguerra Claudia , Correale Pierpaolo , Calvanese Maria Grazia , Falivene Sara , Sangiovanni Angelo , Grassi Roberta , Di Biase Angela , Polifrone Maria Angela , Caraglia Michele , Cappabianca Salvatore , Guida Cesare
Published: March 2021

Introduction: Novel coronavirus (COVID-19) is having a devastating psychological impact on patients, especially patients with cancer. This work aims to evaluate mood disorders of cancer patients undergoing radiation therapy during COVID-19 in comparison with cancer patients who underwent radiation therapy in 2019.

Materials and Methods: We included all the patients undergoing radiation therapy at our department in two-time points (once a week for a month in May 2019) and during the COVID-19 outbreak (in April 2020). All the patients were asked to fulfill a validated questionnaire (STAI-Y1, State trait anxiety inventory scale), the Symptom Distress thermometer (SDT) (from 0 to 10 score), and the Beck Depression Inventory v.2 (BDI-2). We took into account the COVID-19 outbreak and also sex, age, week of radiation treatment, and disease.

Results: We included 458 patients (220 males and 238 females), with a median age of 64 years. STAI-Y1 median score was 40 (mean 41,3, range 19–79), whereas the median score of SDT was five and BDI-2 median score was 11. STAI-Y1, SDT, and BDI-2 were significantly correlated with the COVID-19 outbreak (p < 0,001 for all the tests), sex (p: 0,016 for STAI-Y1, p < 0.001 for SDT, p:0.013 for BDI-2), week of treatment (p: 0.012 for STAI-Y1 and p: 0.031 for SDT), and disease (p:0.015 for STAI-Y1, p < 0.001 for SDT and p:0.020 for BDI-2).

Conclusions: The prevalence of mood disorders in patients undergoing radiation therapy is higher than expected and even higher during the COVID-19 outbreak. These measurements could be useful as a baseline to start medical humanities programs to decrease these scores.
Impact of Divergent Thinking Training on Teenagers’ Emotion and Self-Efficacy During the COVID-19 Pandemic
Authors: Zuo Bin , Wang Qi , Qiao Yalan , Ding Yu , Wen Fangfang
Published: March 2021

Currently due to the COVID-19 pandemic, young people are experiencing a decrease in self-efficacy and an increase in mental illness. Though previous studies have shown that self-efficacy and divergent thinking training are positively related, little is known about the impact of divergent thinking training on self-efficacy and emotions. Therefore, our study seeks this answer to support teenagers injured psychologically during disastrous periods. We randomly assigned 70 students to a 2 (time: pretest, post-test) × 2 (groups: divergent thinking training, controlled) mixed design. Participants in the experimental group were given a 9-day divergent thinking training with the theme of “writing down 10 novel functions of the mask,” while those in the control group spent 10 min each day recording what they ate. The self-efficacy, anxiety, depression, and stress of two groups were measured before and after training. Results showed that, compared to the control group, self-efficacy ceased decreasing while anxiety decreased for the experimental group. These findings confirm the positive effect of divergent thinking on teenagers. Implications and limitations are discussed.
Why Are No Animal Communication Systems Simple Languages?
Authors: Beecher Michael D.
Published: March 2021

Individuals of some animal species have been taught simple versions of human language despite their natural communication systems failing to rise to the level of a simple language. How is it, then, that some animals can master a version of language, yet none of them deploy this capacity in their own communication system? I first examine the key design features that are often used to evaluate language-like properties of natural animal communication systems. I then consider one candidate animal system, bird song, because it has several of the key design features or their precursors, including social learning and cultural transmission of their vocal signals. I conclude that although bird song communication is nuanced and complex, and has the acoustic potential for productivity, it is not productive – it cannot be used to say many different things. Finally, I discuss the debate over whether animal communication should be viewed as a cooperative information transmission process, as we typically view human language, or as a competitive process where signaler and receiver vie for control. The debate points to a necessary condition for the evolution of a simple language that has generally been overlooked: the degree of to which the interests of the signaler and receiver align. While strong cognitive and signal production mechanisms are necessary pre-adaptations for a simple language, they are not sufficient. Also necessary is the existence of identical or near-identical interests of signaler and receiver and a socio-ecology that requires high-level cooperation across a range of contexts. In the case of our hominid ancestors, these contexts included hunting, gathering, child care and, perhaps, warfare. I argue that the key condition for the evolution of human language was the extreme interdependency that existed among unrelated individuals in the hunter-gatherer societies of our hominid ancestors. This extreme interdependency produced multiple prosocial adaptations for effective intragroup cooperation, which in partnership with advanced cognitive abilities, set the stage for the evolution of language.
Home Learning Environments of Children in Mexico in Relation to Socioeconomic Status
Authors: Susperreguy María Inés , Jiménez Lira Carolina , Xu Chang , LeFevre Jo-Anne , Blanco Vega Humberto , Benavides Pando Elia Verónica , Ornelas Contreras Martha
Published: March 2021

We explored the home learning environments of 173 Mexican preschool children (aged 3–6 years) in relation to their numeracy performance. Parents indicated the frequency of their formal home numeracy and literacy activities, and their academic expectations for children’s numeracy and literacy performance. Children completed measures of early numeracy skills. Mexican parent–child dyads from families with either high- or low-socioeconomic status (SES) participated. Low-SES parents (n= 99) reported higher numeracy expectations than high-SES parents (n= 74), but similar frequency of home numeracy activities. In contrast, high-SES parents reported higher frequency of literacy activities. Path analyses showed that operational (i.e., advanced) numeracy activities were positively related to children’s numeracy skills in the high- but not in the low-SES group. These findings improve the understanding of the role of the home environment in different contexts and provide some insights into the sources of the variable patterns of relations between home learning activities and children’s numeracy outcomes. They also suggest that SES is a critical factor to consider in research on children’s home numeracy experiences.
Risk Perception, Unhealthy Behavior, and Anxiety Due to Viral Epidemic Among Healthcare Workers: The Relationships With Depressive and Insomnia Symptoms During COVID-19
Authors: Lee Jukab , Lee Hyuk Joo , Hong Youjin , Shin Yong-Wook , Chung Seockhoon , Park Jangho
Published: March 2021

We aimed to investigate the relationship between mental health problems and unhealthy behaviors among healthcare workers in response to the COVID-19 pandemic. Using an online survey, we collected data on healthcare workers' perception regarding COVID-19 exposure in a work unit. Workers' depression, insomnia, and anxiety symptoms were assessed using the Patient Health Questionnaire-9, Insomnia Severity Index, and Generalized Anxiety Disorder-7 scale, respectively. Work-related stress and anxiety in response to the viral epidemic were measured using the Stress and Anxiety to Viral Epidemic-9 (SAVE-9) scale. We found that work-related stress and anxiety in response to the viral epidemic was associated with female sex, perception of the workplace as being dangerous, and depressive symptoms. Unhealthy behaviors, such as smoking and drinking as coping behaviors during the pandemic, were associated with male sex, young age, depression, and insomnia. During the COVID-19 pandemic, it is necessary to closely observe the patterns of work-related stress and anxiety reactions among healthcare workers to reduce their burnout.
Clinical Efficacy and Cost-Effectiveness of Imagery Rescripting Only Compared to Imagery Rescripting and Schema Therapy in Adult Patients With PTSD and Comorbid Cluster C Personality Disorder: Study Design of a Randomized Controlled Trial
Authors: van den End Arne , Dekker Jack , Beekman Aartjan T. F. , Aarts Inga , Snoek Aishah , Blankers Matthijs , Vriend Chris , van den Heuvel Odile A. , Thomaes Kathleen
Published: March 2021

Background: Posttraumatic stress disorder (PTSD) is a serious and relatively common mental disorder causing a high burden of suffering. Whereas evidence-based treatments are available, dropout and non-response rates remain high. PTSD and Cluster C personality disorders (avoidant, dependent or obsessive-compulsive personality disorder; CPD) are highly comorbid and there is evidence for suboptimal treatment effects in this subgroup of patients. An integrated PTSD and CPD treatment may be needed to increase treatment efficacy. However, no studies directly comparing the efficacy of regular PTSD treatment and treatment tailored to PTSD and comorbid CPD are available. Whether integrated treatment is more effective than treatment focused on PTSD alone is important, since (1) no evidence-based guideline for PTSD and comorbid CPD treatment exists, and (2) treatment approaches to CPD are costly and time consuming. Present study design describes a randomized controlled trial (RCT) directly comparing trauma focused treatment with integrated trauma focused and personality focused treatment.

Methods: An RCT with two parallel groups design will be used to compare the clinical efficacy and cost-effectiveness of “standalone” imagery rescripting (n = 63) with integrated imagery rescripting and schema therapy (n = 63). This trial is part of a larger research project on PTSD and personality disorders. Predictors, mediators and outcome variables are measured at regular intervals over the course of 18 months. The main outcome is PTSD severity at 12 months. Additionally, machine-learning techniques will be used to predict treatment outcome using biopsychosocial variables.

Discussion: This study protocol outlines the first RCT aimed at directly comparing the clinical efficacy and cost-effectiveness of imagery rescripting and integrated imagery rescripting and schema therapy for treatment seeking adult patients with PTSD and comorbid cluster C personality pathology. Additionally, biopsychosocial variables will be used to predict treatment outcome. As such, the trial adds to the development of an empirically informed and individualized treatment indication process.

Clinical Trial registration:ClinicalTrials.gov, NCT03833531.
Prospective Long-Term Cohort Study of Subjects With First-Episode Psychosis Examining Eight Major Outcome Domains and Their Predictors: Study Protocol
Authors: Peralta Victor , Moreno-Izco Lucía , García de Jalón Elena , Sánchez-Torres Ana M. , Janda Lucía , Peralta David , Fañanás Lourdes , Cuesta Manuel J. ,   SEGPEPs Group
Published: March 2021

Background:Our current ability to predict the long-term course and outcome of subjects with a first-episode of psychosis (FEP) is limited. To improve our understanding of the long-term outcomes of psychotic disorders and their determinants, we designed a follow-up study using a well-characterized sample of FEP and a multidimensional approach to the outcomes. The main goals were to characterize the long-term outcomes of psychotic disorders from a multidimensional perspective, to address the commonalities and differential characteristics of the outcomes, and to examine the common and specific predictors of each outcome domain. This article describes the rationale, methods, and design of a longitudinal and naturalistic study of subjects with epidemiologically defined first-admission psychosis.

Methods:Eligible subjects were recruited from consecutive admissions between January 1990 and December 2009. Between January 2018 and June 2021, we sought to trace, re-contact, and re-interview the subjects to assess the clinical course, trajectories of symptoms and functioning, and the different outcomes of psychotic disorders. Since this is a naturalistic study, the research team will not interfere with the subjects' care and treatment. Predictors include antecedent variables, first-episode characteristics, and illness-related variables over the illness course. We assess eight outcome domains at follow-up: psychopathology, psychosocial functioning, self-rated personal recovery, self-rated quality of life, cognitive performance, neuromotor dysfunction, medical and psychiatric comorbidities, and mortality rate. The range of the follow-up period will be 10–31 years with an estimated mean of 20 years. We estimate that more than 50% of the baseline sample will be assessed at follow-up.

Discussion:The study design was driven by the increasing need to refine the ability to predict the different clinical outcomes in FEP, and it aims to close current gaps in knowledge, with a broad approach to both the definition of outcomes and their determinants. To the best of our knowledge, this study is one of the few attempting to characterize the very long-term outcome of FEP and the only study addressing eight major outcome domains. We hope that this study helps to better characterize the long-term outcomes and their determinants, enabling better risk stratification and individually tailored, person-based interventions.
Failure of Healthcare Provision for Attention-Deficit/Hyperactivity Disorder in the United Kingdom: A Consensus Statement
Authors: Young Susan , Asherson Philip , Lloyd Tony , Absoud Michael , Arif Muhammad , Colley William Andrew , Cortese Samuele , Cubbin Sally , Doyle Nancy , Morua Susan Dunn , Ferreira-Lay Philip , Gudjonsson Gisli , Ivens Valerie , Jarvis Christine , Lewis Alexandra , Mason Peter , Newlove-Delgado Tamsin , Pitts Mark , Read Helen , van Rensburg Kobus , Zoritch Bozhena , Skirrow Caroline
Published: March 2021

Background: Despite evidence-based national guidelines for ADHD in the United Kingdom (UK), ADHD is under-identified, under-diagnosed, and under-treated. Many seeking help for ADHD face prejudice, long waiting lists, and patchy or unavailable services, and are turning to service-user support groups and/or private healthcare for help.

Methods: A group of UK experts representing clinical and healthcare providers from public and private healthcare, academia, ADHD patient groups, educational, and occupational specialists, met to discuss shortfalls in ADHD service provision in the UK. Discussions explored causes of under-diagnosis, examined biases operating across referral, diagnosis and treatment, together with recommendations for resolving these matters.

Results: Cultural and structural barriers operate at all levels of the healthcare system, resulting in a de-prioritization of ADHD. Services for ADHD are insufficient in many regions, and problems with service provision have intensified as a result of the response to the COVID-19 pandemic. Research has established a range of adverse outcomes of untreated ADHD, and associated long-term personal, social, health and economic costs are high. The consensus group called for training of professionals who come into contact with people with ADHD, increased funding, commissioning and monitoring to improve service provision, and streamlined communication between health services to support better outcomes for people with ADHD.

Conclusions: Evidence-based national clinical guidelines for ADHD are not being met. People with ADHD should have access to healthcare free from discrimination, and in line with their legal rights. UK Governments and clinical and regulatory bodies must act urgently on this important public health issue.
The Science of Scale for Violence Prevention: A New Agenda for Family Strengthening in Low- and Middle-Income Countries
Authors: Shenderovich Yulia , Lachman Jamie M. , Ward Catherine L. , Wessels Inge , Gardner Frances , Tomlinson Mark , Oliver Daniel , Janowski Roselinde , Martin Mackenzie , Okop Kufre , Sacolo-Gwebu Hlengiwe , Ngcobo Lindokuhle L. , Fang Zuyi , Alampay Liane , Baban Adriana , Baumann Ana A. , de Barros Regina Benevides , Bojo Samuel , Butchart Alexander , Dippenaar Wilmi , Exavery Amon , Fang Xiangming , Ferdinandi Ida , Foran Heather M. , Heinrichs Nina , Hutchings Judy , Kisyombe Daisy , Massetti Greta , Mazak Jaromir , Mbuyi Henry , Singh Pratibha , Polsky Kenneth , Rakotomalala Sabine , Raleva Marija , Savo Richard , Cluver Lucie
Published: March 2021

Ending all violence against children by 2030 is a core part of Sustainable Development Goals 5 and 16. A number of promising violence reduction strategies have been identified in research studies. However, we lack an understanding of the implementation and impact of these programs in respect to their delivery at a large scale or within existing service systems, particularly in low- and middle-income countries (LMICs). We advocate for greater collaboration between researchers, policymakers, donors, governments, non-governmental organizations, and program managers and staff to study how violence prevention programs operate on a large scale. We describe a new initiative aiming to foster such collaborations in the field of family strengthening programs.
Psychological Stress Risk Factors, Concerns and Mental Health Support Among Health Care Workers in Vietnam During the Coronavirus Disease 2019 (COVID-19) Outbreak
Authors: Nguyen Phuong Thi Lan , Nguyen Tien Bao Le , Pham Anh Gia , Duong Khanh Ngoc Cong , Gloria Mac Ardy Junio , Vo Thanh Van , Vo Bay Van , Phung Toi Lam
Published: March 2021

Introduction: Coronavirus disease 2019 (COVID-19) has significantly affected health care workers (HCWs), including their mental health. However, there has been limited evidence on this topic in the Vietnamese context. Therefore, this study aimed to explore COVID-19-related, psychological stress risk factors among HCWs, their concerns and demands for mental health support during the pandemic period.

Methods: We employed a cross-sectional study design with convenience sampling. An online, self-administered questionnaire was used and distributed through social media among medical and non-medical HCWs from April 22 to May 12, 2020. HCWs were categorized either as frontline or non-frontline. We measured the prevalence of psychological stress using the Impact of Event Scale-Revised (IES-R) instrument. Multivariate binary logistic regression analysis was performed to identify risk factors associated with psychological stress among HCWs.

Results: Among the 774 enrolled participants, 761 (98.3%) eligible subjects were included in the analysis. Most respondents were females (58.2%), between 31 and 40 years of age (37.1%), lived in areas where confirmed COVID-19 cases had been reported (61.9%), medical HCWs (59.9%) and practiced being at the frontline (46.3%). The prevalence of stress was 34.3%. We identified significant risk factors such as being frontline HCWs (odds ratio [OR] = 1.77 [95% confidence interval [CI]: 1.17–2.67]), perceiving worse well-being as compared to those before the COVID-19 outbreak [OR = 4.06 (95% CI: 2.15–7.67)], and experiencing chronic diseases [OR = 1.67 (95% CI: (1.01–2.77)]. Majority (73.9%) were concerned about testing positive for COVID-19 and exposing the infection to their families. Web-based psychological interventions that could provide knowledge on managing mental distress and consulting services were highly demanded among HCWs.

Conclusion: The prevalence of psychological stress among HCWs in Vietnam during the COVID-19 pandemic was high. There were also significant risk factors associated with it. Psychological interventions involving web-based consulting services are highly recommended to provide mental health support among HCWs.
The Census of Exoplanets in Visual Binaries: Population Trends from a Volume-Limited Gaia DR2 and Literature Search
Authors: Fontanive Clémence , Bardalez Gagliuffi Daniella
Published: March 2021

We present results from an extensive search in the literature and Gaia DR2 for visual co-moving binary companions to stars hosting exoplanets and brown dwarfs within 200 pc. We found 218 planet hosts out of the 938 in our sample to be part of multiple-star systems, with 10 newly discovered binaries and 2 new tertiary stellar components. This represents an overall raw multiplicity rate of 23.2 ± 1.6 % for hosts to exoplanets across all spectral types, with multi-planet systems found to have a lower stellar duplicity frequency at the 2.2-σ level. We found that more massive hosts are more often in binary configurations, and that planet-bearing stars in multiple systems are predominantly observed to be the most massive component of stellar binaries. Investigations of the multiplicity of planetary systems as a function of planet mass and separation revealed that giant planets with masses above 0.1 MJup are more frequently seen in stellar binaries than small sub-Jovian planets with a 3.6-σ difference, a trend enhanced for the most massive (>7 MJup) short-period (<0.5 AU) planets and brown dwarf companions. Binarity was however found to have no significant effect on the demographics of low- mass planets (<0.1 MJup) or warm and cool gas giants (>0.5 AU). While stellar companion mass appears to have no impact on planet properties, binary separation seems to be an important factor in the resulting structure of planetary systems. Stellar companions on separations <1000 AU can play a role in the formation or evolution of massive, close-in planets, while planets in wider binaries show similar properties to planets orbiting single stars. Finally, our analyses indicate that numerous stellar companions on separations smaller than 1–3 arcsec likely remain undiscovered to this date. Continuous efforts to complete our knowledge of stellar multiplicity on separations of tens to hundreds of AU are essential to confirm the reported trends and further our understanding of the roles played by multiplicity on exoplanets.
Stemness-Associated Markers Are Expressed in Extracranial Arteriovenous Malformation
Authors: Luke Krishnan Claire S. , Brasch Helen D. , Patel Josie , Bockett Nicholas , Paterson Erin , Davis Paul F. , Tan Swee T.
Published: March 2021

Objectives: Arteriovenous malformation (AVM) consists of a nidus with poorly formed low-resistance vessels in place of a functional capillary network. The role of somatic mutations in embryonic stem cells (ESCs) and vascular anomalies and the presence of primitive populations in vascular anomalies led us to investigate the presence of a primitive population in extracranial AVM.

Methods: Extracranial AVM tissue samples from 12 patients were stained for stemness-associated markers OCT4, SOX2, NANOG, KLF4, and c-MYC using immunohistochemical staining. In situ hybridization (ISH) was performed on six tissue samples to determine transcript expression. Western blotting and RT-qPCR were performed on two AVM-derived primary cell lines to determine protein and transcript expression of these markers, respectively. Immunofluorescence staining was performed on two tissue samples to investigate marker co-localization.

Results: Immunohistochemical staining demonstrated the expression of OCT4, SOX2, KLF4, and c-MYC on the endothelium and media of lesional vessels and cells within the stroma of the nidus in all 12 AVM tissue samples. ISH and RT-qPCR confirmed transcript expression of all five markers. Western blotting showed protein expression of all markers except NANOG. Immunofluorescence staining demonstrated an OCT4+/SOX2+/KLF4+/c-MYC+ population within the endothelium and media of the lesional vessels and cells within the stroma of the AVM nidus.

Conclusions: Our findings may suggest the presence of a primitive population within the AVM nidus. Further investigation may lead to novel therapeutic targeting of this population.
Flow Cytometry in the Diagnosis of Canine B-Cell Lymphoma
Authors: Riondato Fulvio , Comazzi Stefano
Published: March 2021

B cell lymphoma (BCL) is a heterogeneous group of lymphoid malignancies which comprise the majority of canine lymphomas. Diffuse large B cell lymphoma is the most common lymphoma subtype in dogs but other subtypes (e.g., marginal zone lymphoma, follicular lymphoma, mantle cell lymphoma, and others) have been described. This review aims to explore the use of flow cytometry to refine the diagnosis of canine BCL. Particular emphasis will be given to the possible identification of peculiar immunotypes, putative prognostic markers, staging and minimal residual disease.
Identification of Dendritic Cell Maturation, TLR, and TREM1 Signaling Pathways in the Brucella canis Infected Canine Macrophage Cells, DH82, Through Transcriptomic Analysis
Authors: Park Woo Bin , Kim Suji , Shim Soojin , Yoo Han Sang
Published: March 2021

Research has been undertaken to understand the host immune response toBrucella canisinfection because of the importance of the disease in the public health field and the clinical field. However, the previous mechanisms governing this infection have not been elucidated. Therefore,in vitromodels, which mimic thein vivoinfection route using a canine epithelial cell line, D17, and a canine macrophage, DH82, were established to determine these mechanisms by performing an analysis of the transcriptomes in the cells. In this study, a coculture model was constructed by using the D17 cell line and DH82 cell line in a transwell plate. Also, a single cell line culture system using DH82 was performed. After the stimulation of the cells in the two different systems infected withB. canis, the gene expression in the macrophages of the two different systems was analyzed by using RNA-sequencing (RNA-seq), and a transcriptomic analysis was performed by using the Ingenuity Pathway Analysis (IPA). Gene expression patterns were analyzed in the DH82 cell line at 2, 12, and 24 h after the stimulation withB. canis. Changes in the upregulated or downregulated genes showing 2-fold or higher were identified at each time point by comparing with the non-stimulated group. Differentially expressed genes (DEGs) between the two culture models were identified by using the IPA program. Generally, the number of genes expressed in the single cell line culture was higher than the number of genes expressed in the coculture model for all-time points. The expression levels of those genes were higher in the single cell line culture (p< 0.05). This analysis indicated that the immune response-related pathways, especially, the dendritic cell maturation, Triggering receptor expression on myeloid cells 1 (TREM1) signaling, and Toll-like receptor (TLR) signaling pathway, were significantly induced in both the culture systems with higherp-values andz-scores. An increase in the expression level of genes related to the pathways was observed over time. All pathways are commonly associated with a manifestation of pro-inflammatory cytokines and early immune responses. However, the Peroxisome proliferator-activation receptor (PPAR) signaling and Liver X Receptor/Retinoid X Receptor (LXR/RXR) signaling associated with lipid metabolism were reduced. These results indicate that early immune responses might be highly activated inB. canisinfection. Therefore, these results might suggest clues to reveal the early immune response of the canine toB. canisinfection, particularly TLR signaling.
Dietary Soybean Oil Supplementation Affects Keel Bone Characters and Daily Feed Intake but Not Egg Production and Quality in Laying Hens Housed in Furnished Cages
Authors: Wei Haidong , Pan Lei , Li Chun , Zhao Peng , Li Jianhong , Zhang Runxiang , Bao Jun
Published: March 2021

To evaluate dietary soybean oil supplementation on production performance, egg quality, and keel bone health in laying hens. Two hundred and four laying hens at 20 weeks of age (WOA) were distributed into 12 cages containing 17 birds each. Birds were either fed a commercial diet (control group, CON) or a diet supplemented with 3% of soybean oil (SO group). Experiments lasted 17 weeks. Body weight, daily feed intake, production performance and egg quality were measured at 25, 29, 33, and 37 WOA. Birds were subsequently assessed for keel bone status by palpation, and keel was excised to measure bone length, microstructure, bone mineral density (BMD), elements contents, and the expression of osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL), collagen type II alpha 1 (COL2α1), periostin (POSTN), and sclerostin (SOST). The results showed that dietary SO supplementation did not affect production performance and egg quality (P > 0.05), but improved body weight of hens at 29 and 37 WOA (P < 0.05), and decreased daily feed intake at 33 and 37 WOA (P < 0.05). Incidence of keel bone damage (especially fracture) was higher in hens of SO group. Keel bone length in birds of SO group was significantly decreased compared to CON (P < 0.05). Keel bone of supplemented hens showed increased trabecular separation at 29 WOA and higher levels of V, Mn, Fe, Se, and Ba at 33 WOA (P < 0.05). Moreover, decreased BMD, trabecular number and thickness were observed in keel bone of laying hens receiving supplementation at 29 and 37 WOA (P < 0.05); decreased levels of Li, Ca, Hg, and TI at 33 WOA and trabecular thickness at 37 WOA (P < 0.05) were also identified. mRNA levels of SOST and RANKL and the ratio of RANKL/OPG mRNA levels were increased in birds fed a SO-supplemented diet (P < 0.05); COL2α1, OPG, and POSTN were downregulated at all sampling points (P < 0.05). Taken together, results indicate that feeding laying hens a diet supplemented with soybean oil can decrease daily feed intake and impair keel bone health but not influence production performance and egg quality.
Response of Apricot Fruit Quality to Protective Netting
Authors: Melgarejo Pablo , Legua Pilar , Martínez-Font Rafael , Martínez-Nicolás Juan José , Sánchez Soriano Joaquín , Carbonell-Barrachina Ángel A. , Hernández Francisca
Published: March 2021

The cultivation of fruit trees in protected environments is a technique that has been developed in recent years for peaches and nectarines, but not for apricots. This study was conducted to investigate the chemical composition of the fruits and their quality indices variations of the variety ‘Mikado’ as a function of its cultivation under a protective net or outdoors. As a practical agronomic assay, a homogeneous experimental plot was used in this study, where half of the apricot trees were cultivated under protective netting, and the other half without it. The data showed that for the total yield, no statistically significant differences were found with respect to ‘Mikado’ trees cultivated with or without a net. The trees cultivated under protective nets showed higher fruit weights. The results showed that the technique of using protective nets for the cultivation of extra-early apricot ‘Mikado’ is a profitable alternative for farmers, and their use does not affect fruit production or quality.
Impact of Rice Straw Mulch on Soil Physical Properties, Sunflower Root Distribution and Yield in a Salt-Affected Clay-Textured Soil
Authors: Paul Priya Lal Chandra , Bell Richard W , Barrett-Lennard Edward G. , Kabir Enamul
Published: March 2021

Puddling of clay soils for rice transplanting causes a loss of soil structure and vertical shrinkage cracks that are hypothesized to hamper sunflower root growth in the following dry season. To alleviate soil constraints for sunflower root growth and yield, we examined the effects of three levels of mulch and two irrigation regimes in the dry season on a clay-textured soil in the coastal zone of Bangladesh. These treatments were no-mulch, rice straw mulch at 5 t ha−1 and 10 t ha−1, irrigation applied to the field capacity (I1) and a water supply double that of the I1 treatment (I2). The rice straw mulch significantly increased soil water content by 3–9% and decreased soil penetration resistance by 28–77% and crack volume by 84–91% at A 0–30 cm soil depth relative to the no-mulch treatment. The better root development with the rice straw mulch increased sunflower yield by 23%. No benefit or further reduction in soil penetration resistance or yield improvement was obtained from increasing the level of mulch from 5 to 10 t ha−1 or the volume of irrigation water. It is concluded that ameliorating soil constraints by mulch application led to better root growth in the upper root zone and the increased yield in the clay soil.
HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1
Authors: Gramlich Marius , Hays Henry C. W. , Crichton Scott , Kaiser Philipp D. , Heine Anne , Schneiderhan-Marra Nicole , Rothbauer Ulrich , Stoll Dieter , Maier Sandra , Zeck Anne
Published: March 2021

Annexin-A1 (ANXA1) belongs to a class of highly homologous Ca2+-dependent phospholipid-binding proteins. Its structure consists of a core region composed of four homologous repeats arranged in a compact, hydrolysis-resistant structure and an N-terminal region with a Ca2+-dependent conformation. ANXA1 is involved in several processes, including cell proliferation, apoptosis, metastasis, and the inflammatory response. Therefore, the development of antibodies blocking selected regions on ANXA1 holds great potential for the development of novel therapeutics treating inflammatory and cancer diseases. Here, we report the interaction site between an ANXA1-specific antibody known to inhibit T cell activation without adverse cytotoxic effects and ANXA1 using amide hydrogen–deuterium exchange mass spectrometry (HDX-MS). For the epitope determination, we applied two bottom-up HDX-MS approaches with pepsin digestion in solution and immobilized on beads. Both strategies revealed the interaction region within domain III of ANXA1 in Ca2+-bound conformation. The antibody-binding region correlates with the hydrophobic binding pocket of the N-terminal domain formed in the absence of calcium. This study demonstrates that even cryptic and flexible binding regions can be studied by HDX-MS, allowing a fast and efficient determination of the binding sites of antibodies which will help to define a mode of action profile for their use in therapy.
Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review
Authors: Yan Yumei , Li Xing , Zhang Chunhong , Lv Lijuan , Gao Bing , Li Minhui
Published: March 2021

Alkaloids are nitrogen-containing heterocyclic compounds typically isolated from plants. They represent one of the most important types of natural products because of their large number and structural diversity and complexity. Based on their chemical core structures, alkaloids are classified as isoquinolines, quinolines, indoles, piperidine alkaloids, etc. In-depth analyses of alkaloids have revealed their antibacterial activities. To date, due to the widespread use of antibiotics, the problem of drug-resistant bacterial infections has been gradually increasing, which severely affects the clinical efficacy of antibacterial therapies and patient safety. Therefore, significant research efforts are focused on alkaloids because they represent a potentially new type of natural antibiotic with a wide antibacterial spectrum, rare adverse reactions, and a low tendency to produce drug resistance. Their main antibacterial mechanisms include inhibition of bacterial cell wall synthesis, change in cell membrane permeability, inhibition of bacterial metabolism, and inhibition of nucleic acid and protein synthesis. This article reviews recent reports about the chemical structures and the antibacterial activities and mechanisms of alkaloids. The purpose is to solve the problem of bacterial resistance and to provide a certain theoretical basis and research ideas for the development of new antibacterial drugs.
Antibiotic Resistance Patterns of Bacterial Isolates from Neonatal Sepsis Patients at University Hospital of Leipzig, Germany
Authors: Tessema Belay , Lippmann Norman , Knüpfer Matthias , Sack Ulrich , König Brigitte
Published: March 2021

Neonatal sepsis caused by resistant bacteria is a worldwide concern due to the associated high mortality and increased hospitals costs. Bacterial pathogens causing neonatal sepsis and their antibiotic resistance patterns vary among hospital settings and at different points in time. This study aimed to determine the antibiotic resistance patterns of pathogens causing neonatal sepsis and to assess trends in antibiotic resistance. The study was conducted among neonates with culture proven sepsis at the University Hospital of Leipzig between November 2012 and September 2020. Blood culture was performed by BacT/ALERT 3D system. Antimicrobial susceptibility testing was done with broth microdilution method based on ISO 20776-1 guideline. Data were analyzed by SPSS version 20 software. From 134 isolates, 99 (74%) were gram positive bacteria. The most common gram positive and gram negative bacteria were S. epidermidis, 51 (38%) and E. coli, 23 (17%), respectively. S. epidermidis showed the highest resistance to penicillin G and roxithromycin (90% each) followed by cefotaxime, cefuroxime, imipenem, oxacillin, and piperacillin-tazobactam (88% each), ampicillin-sulbactam (87%), meropenem (86%), and gentamicin (59%). Moreover, S. epidermidis showed raising levels of resistance to amikacin, gentamicin, ciprofloxacin, levofloxacin, moxifloxacin, and cotrimoxazol. Gram positive bacteria showed less or no resistance to daptomycin, linezolid, teicoplanin, and vancomycin. E. coli showed the highest resistance to ampicillin (74%) followed by ampicillin-sulbactam (52%) and piperacillin (48%). Furthermore, increasing levels in resistance to ampicillin, ampicillin-sulbactam, piperacillin, and cefuroxime were observed over the years. Encouragingly, E. coli showed significantly declining trends of resistance to ciprofloxacin and levofloxacin, and no resistance to amikacin, colistin, fosfomycin, gentamicin, imipenem, piperacillin-tazobactam, and tobramycin. In conclusion, this study demonstrates that gram positive bacteria were the leading causes of neonatal sepsis. Bacterial isolates were highly resistant to first and second-line empiric antibiotics used in this hospital. The high levels of antibiotic resistance patterns highlight the need for modifying empiric treatment regimens considering the most effective antibiotics. Periodic surveillance in hospital settings to monitor changes in pathogens, and antibiotic resistance patterns is crucial in order to implement optimal prevention and treatment strategies.
Automotive Body Shop Design Problems Using Meta-Models Considering Product-Mix Change and Reconfiguration Strategy
Authors: Moon Dug Hee , Kim Dong Ok , Shin Yang Woo
Published: March 2021

The estimation of production rate (or throughput) is important in manufacturing system design. Herein, we consider the manufacturing system of an automotive body shop in which two types of car are produced, and one car (engine car) is substituted by the other car (electric car) gradually. In this body shop, two different underbody lines are installed because the underbody structures of the two types of cars differ completely; however, the side body line and main body line are shared by the two cars. Furthermore, we assume that the underbody lines are reconfigurable based on an increase in the product mix of the electric car. A simulation-based meta-model, which is in the form of a quadratic polynomial function, is developed to estimate the production rate. In the meta-modelling process, we group some buffer locations and represent them as one variable to reduce the number of variables included in the meta-model. Subsequently, the meta-models have been used to optimize two types of buffer allocation problems, and optimal solutions are obtained easily.
Silhouettes from Real Objects Enable Realistic Interactions with a Virtual Human in Mobile Augmented Reality
Authors: Kim Hanseob , Ali Ghazanfar , Pastor Andréas , Lee Myungho , Kim Gerard J. , Hwang Jae-In
Published: March 2021

Realistic interactions with real objects (e.g., animals, toys, robots) in an augmented reality (AR) environment enhances the user experience. The common AR apps on the market achieve realistic interactions by superimposing pre-modeled virtual proxies on the real objects in the AR environment. This way user perceives the interaction with virtual proxies as interaction with real objects. However, catering to environment change, shape deformation, and view update is not a trivial task. Our proposed method uses the dynamic silhouette of a real object to enable realistic interactions. Our approach is practical, lightweight, and requires no additional hardware besides the device camera. For a case study, we designed a mobile AR application to interact with real animal dolls. Our scenario included a virtual human performing four types of realistic interactions. Results demonstrated our method’s stability that does not require pre-modeled virtual proxies in case of shape deformation and view update. We also conducted a pilot study using our approach and reported significant improvements in user perception of spatial awareness and presence for realistic interactions with a virtual human.
Environmental Impact of Energy Systems Integrated with Electrochemical Accumulators and Powered by Renewable Energy Sources in a Life-Cycle Perspective
Authors: Stoppato Anna , Benato Alberto , De Vanna Francesco
Published: March 2021

The aim of this study is to assess the environmental impact of storage systems integrated with energy plants powered by renewable sources. Stationary storage systems proved to be a valid solution for regulating networks, supporting frequency, and managing peaks in electricity supply and demand. Recently, their coupling with renewable energy sources has been considered a strategic means of exploiting their high potential since it permits them to overcome their intrinsic uncertainty. Therefore, the storage systems integration with distributed generation can improve the performance of the networks and decrease the costs associated with energy production. However, a question remains regarding the overall environmental sustainability of the final energy production. Focusing on electrochemical accumulators, the problems mainly concern the use of heavy metals and/or impacting chemical components of storage at the center of environmental hazard debates. In this paper, an environmental assessment from a life-cycle perspective of the hybrid energy systems powered by fossil and renewable sources located on two non-interconnected minor islands is presented. Existing configurations are compared with new ones obtained with the addition of batteries for the exploitation of renewable energy. The results show that, for batteries, the assembly phase, including raw material extraction, transport, and assembly, accounts for about 40% of the total, while the remaining part is related to end-of-life processes. The reuse and recycling of the materials have a positive effect on overall impacts. The results also show that the overall impact is strongly related to the actual energy mix of the place where batteries are installed, even if it is usually lower than that of the solution without the batteries. The importance of a proper definition of the functional unit in the analysis is also emphasized in this work.
Optimizing Support Locations in the Roof–Column Structural System
Authors: Meng Xianchuan , Lee Ting-Uei , Xiong Yulin , Huang Xiaodong , Xie Yi Min
Published: March 2021

The roof–column structural system is utilized for many engineering and architectural applications due to its structural efficiency. However, it typically requires column locations to be predetermined, and involves a tedious trial-and-error adjusting process to fulfil both engineering and architectural requirements. Finding efficient column distributions with the aid of computational methods, such as structural optimization, is an ongoing challenge. Existing methods are limited, with continuum methods involving the generation of undesired complex shapes, and discrete methods involving a time-consuming process for optimizing columns’ spatial order. This paper presents a new optimization method to design the distribution of a given number of vertical supporting columns under a roof structure. A computational algorithm was developed on the basis of the optimality-criterion (OC) method to preserve and removed candidate columns pre-embedded with design requirements. Three substrategies are presented to improve optimizer performance. The effectiveness of the new method was validated with a range of roof–column structural models. Treating column locations as design variables provides opportunities to significantly improve structural performance.