Evaluation of Double Ovsynch Protocol in Acyclic Jaffarabadi Heifers and Buffaloes with Respect to Ovarian Dynamics, Hormonal Profile and Fertility
Authors: Raval R. J. , Vala K. B. , Padodara R. J. , Dhami A. J. , Kavani F. S. 
Published: November 2020

Background: Anestrus is one of the most commonly encountered infertility problems in cattle and buffalo in India. Jaffarabadi is one of the heaviest buffalo breeds of the world and is a native of Saurashtra region of Gujarat. The breed is known for poor reproductive efficiency. To improve its reproductive efficiency, this study was undertaken on acyclic Jaffarabadi animals employing double Ovsynch protocol and its assessment through ovarian dynamics and blood biochemical and endocrine profile on a farm.
Methods: The study included acyclic post-pubertal Jaffarabadi heifers (age 42±2 and 48±2 months; Gr-I and Gr-II, n=6 each) and the postpartum lactating acyclic buffaloes (Gr-III, n=6) using a double Ovsynch protocol, which consisted of i/m injections of 20 µg Buserelin acetate on days 0, 10, 17 and 26 and 500 µg Cloprostenol sodium on days 7 and 24, with a timed insemination on day 27. Ovarian dynamics was monitored by performing ultrasonography using real-time B-mode ultrasound scanner together with blood sampling for hormonal and biochemical profile on each day of hormone therapy and then on days 0, 12, 21 and 35 post-AI. The animals inseminated at induced estrus/FTAI were followed for return to estrus, if any. Pregnancy was confirmed in non-return cases on day 35 by ultrasonography and on day 70 by per rectal examination. The findings on ovarian dynamics, plasma endocrine and biochemical profile were compared statistically between groups and periods and fertility rates between groups.
Result: In animals of treatment group I and II, a significant (p less than 0.05) increase in numbers of small follicles was observed on day 26. Number of large sized follicles was significantly (p less than 0.05) higher on day 26 in comparison to day 0 and day 7 in group III animals. Large and subordinate follicular diameters increased gradually, but the differences between periods were significant (p less than 0.001) only in group III with the highest diameter of large follicle on day 17. Plasma FSH and LH concentrations differed significantly (p less than 0.001) among groups at all-time intervals. Plasma LH in group I and III differed significantly (p less than 0.001). Plasma estrogen level was significantly (p less than 0.05) higher in group II than group III. Plasma progesterone concentrations in group I and II animals were significantly higher on day 35 post-AI. Plasma insulin levels were significantly (p less than 0.01) lower on all days for group III animals than group I and II. Blood glucose level was significantly (p less than 0.001) higher in group I on day 17 as compared to group II and III. Plasma total cholesterol was significantly (p less than 0.05) higher in group III as compared to group I and II. The conception rates at first service/FTAI in double Ovsynch treated animals of group I, II and III were 66.66, 83.33 and 16.66 %, respectively. Thus it was concluded that ovarian structures and the plasma endocrine profile reflected the ovarian response to different hormonal injections and that double Ovsynch protocol could be a better choice for improving conception rate in post-pubertal acyclic Jaffarabadi buffalo heifers as compared to multiparous acyclic buffaloes.