bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel CDKL5 Mutations in Czech Patients with Phenotypes of Atypical Rett Syndrome and Early-Onset Epileptic Encephalopathy
Authors: Záhoráková Daniela , Langová M. , Brožová K. , Laštůvková J. , Kalina Z. , Rennerová L. , Martásek P.
Published: April 2024

The X-linked CDKL5 gene, which encodes cyclin-dependent kinase-like 5 protein, has been implicated in early-onset encephalopathy and atypical Rett syndrome with early-onset seizures. The CDKL5 protein is a kinase required for neuronal development and morphogenesis, but its precise functions are still largely unexplored. Individuals with CDKL5 mutations present with severe global developmental delay, intractable epilepsy, and Rett-like features. A clear genotype-phenotype correlation has not been established due to an insufficient number of reported cases. The aim of this study was to analyse the CDKL5 gene in Czech patients with early-onset seizures and Rett-like features. We performed mutation screening in a cohort of 83 individuals using high-resolution melting analysis, DNA sequencing and multiplex ligation- dependent probe amplification. Molecular analyses revealed heterozygous pathogenic mutations in three girls with severe intellectual disability and intractable epilepsy starting at the age of two months. All three identified mutations, c.637G>A, c.902_977+29del105, and c.1757_1758delCT, are novel, thus significantly extending the growing spectrum of known pathogenic CDKL5 sequence variants. Our results support the importance of genetic testing of the CDKL5 gene in patients with early-onset epileptic encephalopathy and Rett-like features with early-onset seizures. This is the first study referring to molecular defects of CDKL5 in Czech cases.
X-Chromosome Inactivation Analysis in Different Cell Types and Induced Pluripotent Stem Cells Elucidates the Disease Mechanism in a Rare Case of Mucopolysaccharidosis Type II in a Female
Authors: Řeboun M. , Rybová J. , Dobrovolný R. , Včelák J. , Veselková T. , Štorkánová G. , Mušálková D. , Hřebíček M. , Ledvinová J. , Magner M. , Zeman J. , Pešková K. , Dvořáková Lenka
Published: April 2024

Mucopolysaccharidosis type II (MPS II) is an X-linked lysosomal storage disorder resulting from deficiency of iduronate-2-sulphatase activity. The disease manifests almost exclusively in males; only 16 symptomatic heterozygote girls have been reported so far. We describe the results of X-chromosome inactivation analysis in a 5-year-old girl with clinically severe disease and heterozygous mutation p.Arg468Gln in the IDS gene. X inactivation analysed at three X-chromosome loci showed extreme skewing (96/4 to 99/1) in two patient’s cell types. This finding correlated with exclusive expression of the mutated allele. Induced pluripotent stem cells (iPSC) generated from the patient’s peripheral blood demonstrated characteristic pluripotency markers, deficiency of enzyme activity, and mutation in the IDS gene. These cells were capable of differentiation into other cell types (cardiomyocytes, neurons). In MPS II iPSC clones, the X inactivation ratio remained highly skewed in culture conditions that led to partial X inactivation reset in Fabry disease iPSC clones. Our data, in accordance with the literature, suggest that extremely skewed X inactivation favouring the mutated allele is a crucial condition for manifestation of MPS II in females. This suggests that the X inactivation status and enzyme activity have a prognostic value and should be used to evaluate MPS II in females. For the first time, we show generation of iPSC from a symptomatic MPS II female patient that can serve as a cellular model for further research of the pathogenesis and treatment of this disease.
Efectividad de una intervención psicosocial con víctimas de violencia política
Authors: Santamaría-Uribe Laura Sofía , Rey-Gómez Nathalia , Pulido-Escobar Paola Andrea , Martínez-Parra Miguel Ángel , Acosta-Peña María Camila , Rodríguez-Prieto Astrid Carolina , Barreto-Zambrano María Luisa , Aguilar-Pardo David Ricardo , Barreto-Galeano María Idaly
Published: April 2024

La realidad política y social de Colombia ha sido permeada por estructuras de poder y dominación de modo permanente e histórico con la presencia de distintas formas de violencia. En el ámbito político, ese uso de la violencia como medio para imponer, cambiar o preservar determinado orden social se conoce como violencia política (Barreto & Borja, 2007) y se constituye en un eje de investigación que aborda, entre otros, las consecuencias que impactan en la vida de las personas en los ámbitos individual y colectivo (Cancimance, 2013). Al respecto, Díaz Gómez (2007) incluye en las agendas de investigación de la psicología política en Latinoamérica, la psicología y los derechos humanos con una particularidad que destaca la importancia de acompañar a las víctimas de violencia política mediante “procesos terapéuticos o de restitución psicológica” (p. 12), lo cual es de gran relevancia en un país como Colombia, cuyas afectaciones a la salud mental y psicosocial de las personas provienen de hechos victimizantes asociados a hostigamientos, atentados, combates y enfrentamientos (97.913), situaciones de amenaza (627.301), delitos contra la libertad (37.896), desplazamiento forzado (9.483.468), desaparición forzada (204.198), homicidios (1.180.892), secuestros (39.089), tortura (10.595), actividades de vinculación de niñas, niños y adolescentes (9.959), lesiones personales físicas (15.257) y de violencia psicológica (15.216), entre otros (Sistema Nacional de Atención y Reparación Integral a las Víctimas [SNARIV], 2022).
Ciudades para la vida digna: apuestas desde la conciencia política para la apropiación y defensa del territorio. El caso de Bucaramanga y su Área Metropolitana
Authors: Cortés Millán Germán Andrés
Published: April 2024

El texto presente, cuya finalidad radica en exponer la experiencia investigativa que tuvo como escenario la ciudad de Bucaramanga y su Área Metropolitana, y como protagonistas a organizaciones y a procesos colectivos vinculados a la problematización crítica de las políticas de gestión urbana, tiene la siguiente estructura organizativa. En primera instancia se hace una referencia problémica del asunto, incluyendo cada uno de los aspectos sociales, económicos y políticos asociados, de manera directa, a la implementación histórica del modelo neoliberal de la gestión y la planeación urbana, que van desde la concepción misma del espacio habitado, hasta la formalización de políticas sociourbanísticas desplegadas e instrumentalizadas sistemáticamente. Posterior a esto, se hace alusión a los elementos conceptuales que soportan la reflexión crítica y el análisis que, para este caso, se relacionan con el campo de la psicología política como son la construcción social del territorio, el derecho a la ciudad, la acción colectiva y la conciencia política. Para el tercer apartado, se describe el marco metodológico del proyecto, el cual se soporta desde una tipología cualitativa, descriptiva e interpretativa en las que se entrecruzan las versiones narrativas de los participantes, con las expresiones cartográficas realizadas en terreno, junto a las comunidades. Posteriormente, se presenta el análisis de la información recogida, en la que se triangulan los componentes categoriales propios del modelo de conciencia política (identidad colectiva para el derecho a la ciudad, valores y creencias sobre el territorio y reconocimiento del adversario), las narrativas en contexto y los referentes conceptuales considerados. Ya como último apartado del capítulo, se incluyen las discusiones y conclusiones, en las que finalmente se articulan y se contrastan las distintas versiones teóricas, con las apuestas comunitarias sobre los conflictos territoriales que, para el caso de Bucaramanga, representan un referente respecto a las formas de significación, interlocución y uso de la ciudad.
Using analytical performance specifications in a medical laboratory
Authors: Jones Graham Ross Dallas
Published: April 2024

Analytical performance specifications (APS) are used for the quantitative assessment of assay analytical performance, with the aim of providing information appropriate for clinical care of patients. One of the major locations where APS are used is in the routine clinical laboratory. These may be used to assess and monitor assays in a range of settings including method selection, method verification or validation, external quality assurance, internal quality control and assessment of measurement uncertainty. The aspects of assays that may be assessed include imprecision, bias, selectivity, sample type, analyte stability and interferences. This paper reviews the practical use of APS in a routine clinical laboratory, using the laboratory I supervise as an example.
The distributional properties of long nominal compounds in scientific articles: an investigation based on the uniform information density hypothesis
Authors: Gamboa John , Braun Kristina , Järvikivi Juhani , Allen Shanley E. M.
Published: April 2024

Nominal compounds are a structure commonly used in scientific texts. Despite their commonality, very little is known about how they are distributed in scientific articles. Based on the Uniform Information Density hypothesis, which states that speakers communicate information at a constant rate, avoiding peaks and troughs of information transmission, we predict that nominal compounds should cluster toward the end of scientific texts, be preceded by supporting text that facilitates their understanding, and be repeated often after their first use. In this paper, we examine these predictions through a quantitative and a qualitative analysis of a corpus of scientific papers from the fields of Biology, Economics and Linguistics. While our investigation did not reveal definitive findings for the first and third predictions above, it did produce supporting evidence in favor of our second prediction, thus advancing our understanding of NC use and the choices speakers make when transmitting information.
Residual, sequential extraction, and ecological risk assessment of some metals in ash from municipal solid waste incineration, Vietnam
Authors: Mai Ngo Tra , Thi Tham Trinh , Thuy Hang Nguyen Thi , Thi Hong Khuat , Lien Nghiem Thi Ha , Nghia Nguyen Trong , Hang Phan Thi Thanh , Toan Vu Duc , Tap Van Huu , Van Thang Duong , Ly Do Thao
Published: April 2024

Incineration plants for electricity generation have offered a solution to the challenges of domestic solid waste treatment in many countries. However, their emissions, fly ash (FA) and bottom ash (BA), have had a detrimental impact on environmental quality. This study investigated the morphology, element composition, and concentration of metals in FA and BA from municipal solid waste incineration plants using scanning electronic microscopy/energy-dispersive X-ray and inductively coupled plasma mass spectrometry techniques. It also evaluated the distribution ratios of metal species across five fractions: exchangeable, carbonate-bound, oxide-bound, organic-bound, and sulfide-bound, and residual. The total metal content in both FA and BA was comparable, with calcium having the highest concentration (28,170–135,130 mg·kg−1 dry weight) and silver having the lowest (5.26–19.3 mg·kg−1 dry weight). However, the percentage proportion of metals differed between the extracted fractions. Except for cadmium in FA, ecological risk assessment indicated low direct bioavailability and potential risk of metals in both FA and BA. These findings contribute to the hazard assessment of FA and BA generated from waste incinerators and provide a scientific basis for developing treatment techniques for this type of waste.
Enhanced sonocatalytic degradation of Acid Red 27 with Fe2O3 catalyst: a kinetic study
Authors: Abad Suha , Aziz Ruqaiya , Khalil Mohd. Junaid , Rahman Muhammad Muhitur , Hossain Mohammad M.
Published: April 2024

This study is focused on elucidating the potential effectiveness of degradation as a method to eliminate dyes from aqueous systems. Specifically, it delves into the influence of ultrasound energy on the degradation kinetics of a dye. The research findings underscore the notable impact of ultrasound energy in accelerating the reaction rate constant (k
p), with the degradation kinetics exhibiting a conformity to first-order kinetics. An integral aspect of the study involves the establishment of a robust relationship between time and concentration by integrating the equation governing the degradation of the dye. Additionally, the determination of the rate constant, derived from the gradients of the graphs, attests to the model’s fitting accuracy. Intriguingly, the outcomes of this analysis reveal no discernible structural changes in the dye. The accuracy of the model is further underscored by the establishment of linear relations derived from experimental data. Summarily, this kinetic study provides invaluable insights into the multifaceted impact of ultrasound energy and the Fe2O3 catalytic influence on both the degradation kinetics of the dye. The comprehensive nature of the investigation enhances our understanding of the intricate processes involved, contributing significantly to the broader field of water treatment and dye removal from aqueous environments.
Far-field mapping and efficient beaming of second harmonic by a plasmonic metagrating
Authors: Verneuil Augustin , Di Francescantonio Agostino , Zilli Attilio , Proust Julien , Béal Jérémie , Petti Daniela , Finazzi Marco , Celebrano Michele , Baudrion Anne-Laure
Published: April 2024

We study numerically and experimentally the second-harmonic generation (SHG) from rectangular metagratings of V-shaped gold nanoantennas. We show that by carefully engineering the array pitch to steer the diffraction orders toward the single antenna emission, the extracted signal is maximized. This enhancement is attributed to the angular overlap between the radiation pattern and array factor and is comparable to the improvement yielded by the coupling of surface lattice resonances (SLRs) with local modes. Moreover, we demonstrate a simple technique to experimentally reconstruct the emission diagram of an antenna from measurements of the collective grating response as a function of the excitation angle. Excellent agreement is obtained with simulations when the sample is immersed either in air or in water, which is crucial in view of future sensing application. Thanks to the high signal-to-noise ratio and low dependence on the statistical particle dispersity, this method constitutes an effective alternative to back-focal plane imaging when very weak signals such as SHG are involved.
Influence of spent liquor obtained from SO2–ethanol–water (SEW) fractionation of Eucalyptus on drifting sands stabilization
Authors: Firouzabadi Mohammadreza Dehghani , Tatari Aliasghar
Published: April 2024

The utilization of mulch stands as a paramount approach in the management of wind erosion and the stabilization of soil and drifting sands. This study aimed to explore the impact of various concentrations of spent liquor (20 %, 30 %, and 50 % v/v) derived from SO2–ethanol–water (SEW) fractionation of Eucalyptus wood on the physical and mechanical properties of sand. These properties encompassed moisture content, thickness, temperature, electrical conductivity (EC), wind erodibility, penetration resistance, and seed germination. The findings revealed that the highest compressive strength (0.76 MPa) was attained with mulch consisting of 50 % SEW spent liquor, resulting in a 3.3-fold increase in penetration resistance compared to the control treatment. Furthermore, the 20 % concentration of spent liquor did not adversely affect the germination of black saxaul (Haloxylon ammodendron), whereas the lowest seed germination rate was associated with the 50 % concentration. Based on the measured parameters, the optimal mulch treatment for stabilizing drifting sands was identified as mulch with a 50 % (v/v) concentration. This study underscores the efficacy of SEW spent liquor in dust control and mitigating its environmental impacts, thus highlighting its potential in sustainable soil management practices.
Green synthesis of CdS/flaxseed mucilage nanocomposite films using gamma irradiation for packaging applications
Authors: Sokary Rehab , Raslan Heba A. , Fathy Rasha Mohammad
Published: April 2024

The most common ways to produce nanoparticles are through chemical and physical processes, which can be expensive and environmentally hazardous. Using plant extracts (green synthesis) as reducing and capping agents is a simple, cost-effective, and environmentally friendly method of lowering the usage of dangerous chemicals in the synthesis of metal nanoparticles. This study covers the environmentally friendly synthesis of cadmium sulphide nanoparticles (CdS NPs) using a blend of flaxseed extracts (FM), polyvinyl alcohol (PVA), and chitosan (Cs). The composites are then exposed to gamma irradiation at doses of 20 kGy and 40 kGy. UV–VIS absorption spectroscopy, SEM, HRTEM, EDX, and FTIR were used to analyse the produced nanocomposite films. UV–Vis absorption spectra showed considerable surface Plasmon resonance (SPR) bands at 396–440 nm, indicating that CdS NPs had been successfully synthesized. A progressive red shift in wavelength was noted, along with the broadening of the absorption band as the irradiation dose increased. Transmission electron microscopy pictures revealed that the generated CdS nanostructures were dispersed as spherical nanoparticles with remarkable structural homogeneity. Tensile strength and elongation measurements of the films revealed that the inclusion of CdS NPs improved their mechanical properties. The addition of CdS NPs to the current blends limits biodegradation in soil. Thermal gravimetric analysis findings showed that CdS NPs included in FM/PVA films had improved thermal stability. The antimicrobial activities of the tested films were performed against Staphylococcus aureus, Escherichia coli, and Candida albicans. The results revealed that all of the films exhibited more antibacterial activity against S. aureus than the two others, with the highest activity observed in nanocomposites with a high concentration of CdS.
A new uranyl silicate sheet derived from phosphuranylite topology in the structure of Cs4[(UO2)5(SiO3OH)2O2F4]
Authors: Nazarchuk Evgeny V. , Siidra Oleg I. , Charkin Dmitri O. , Tagirova Yana G.
Published: April 2024

A new uranyl silicate Cs4[(UO2)5(SiO3OH)2O2F4] (1), was obtained via a hydrothermal route. The new compound is monoclinic, P21/n, a = 8.3870(2), b = 13.4612(2), c = 10.9503(2) Å, β = 91.223(2)°, V = 1236.00(4) Å3; the structure has been solved and refined down to R
1 = 0.022. Therein, the phosphouranylite units (
PU
s) associate into a new type of uranyl-silicate layers, [(UO2)5(SiO3OH)2O2F4]4−, which interleave with the Cs+ cations. Topological analysis of
PU
based structures indicates that these layers in 1 provide a unique example of complexes constructed only via association of the
PU
and not involving other building units.
Low-temperature heat capacity and absolute entropy of yttrium(III) bromide (YBr3)
Authors: Burkmann Konrad , Habermann Franziska , Gumeniuk Roman , Mertens Florian
Published: April 2024

The heat capacity of yttrium(III) bromide (YBr3) in the temperature range from 2 K to 300 K has been determined using a Physical Property Measurement System (PPMS). From these data the absolute entropy of the compound at 298.15 K has been derived.
The Influence of Age and Work Experience on Lumbar Spine Pain among the Nursing Staff
Authors: Antczak-Komoterska Anna , Nowakowska Lilla , Haor Beata , Kochman Dorota
Published: April 2024

Introduction. Currently, back pain syndromes are considered to be widely known problems in the medical, but above all, social and economic aspects. The specific nature of the profession performed by nurses puts them among the people most at risk of spine dysfunction.

Aim. The aim of this study is to assess the occurrence of pain in the lumbar spine pain among nurses working at the Provincial Specialist Hospital in Włocławek.

Material and Methods. The research was conducted among 120 nurses working in hospital wards of the Provincial Specialist Hospital in Włocławek. The research used an own survey questionnaire and the Revised Oswestry Low Back Pain Disability Scale (ODI) — the Polish version. Consent to conduct the research was obtained from the Management of the Provincial Specialist Hospital in Włocławek and the Bioethics Committee of the State Academy of Applied Sciences in Włocławek.

Results. The majority of respondents indicated that they connect back pain with work — 104 people (86.7%) and received the results of slight limitations — 60 people (50.0%). Most respondents indicated that they had experienced pain for 1–5 years — 42 people (35.0%). Respondents aged up to 45 years indicated that they had been experiencing pain for 1–5 years — 15 people (48.4%). In the group with 30–40 years of experience, the most respondents indicated that they had more than 10 years of experience — 19 people (34.5%). In the group with 30–40 years of experience, the majority indicated that often several times a month — 21 people (38.2%). The highest degree of disability was recorded at the age of 56–62, serious disruptions — 8 people (33.3%), and at the age of 51–55, serious disruptions — 6 people (14.03%).

Conclusions. The work performed affects the occurrence of pain in the lumbar spine. Age groups up to 45 years and 46–50 years have been experiencing pain for 5–10 years. Pain is most often experienced by respondents with 30–40 years of work experience. The vast majority of respondents experience slight limitations. The highest rate of disability is experienced by nurses aged 51–55. (JNNN 2023;12(4):164–169)
Seed Science and Technology. Volume 52 Issue 1 (2024)
Authors: Hay Fiona R.
Published: April 2024

As the International Seed Testing Association (ISTA) celebrates its 100-year anniversary in 2024, we can also commend its specific role in supporting research on seeds. Indeed, one of ISTA's objectives is "To actively promote research and dissemination of knowledge in seed science and technology, for the sampling, testing, storing, processing and distribution of seeds". One of the main ways in which scientific knowledge of seeds is disseminated by ISTA is through its scientific journal,Seed Science and Technology, which itself celebrated its 50thvolume in 2022 with a special issue of review articles and editorial (Jones, 2022). The review articles covered some of the history of research on particular aspects of seed testing, and gave up-to-date summaries of our current understanding of topics such as vigour, seed storage, tetrazolium testing, etc. Collectively, they cite many original research papers published inSeed Science and Technology. Since 2003,Seed Science and Technologyhas been available electronically at https://www.ingentaconnect.com/content/ista/sst, and articles published since 2018 are open-access, meaning that they are available for anyone to read for free. The 1973 to 2002 back issues of SST are available from ISTA at publication. sales@ista.ch. The articles in this latest issue of SST will similarly each advance our understanding of seed processes and responses, continuing to contribute to the body of knowledge on seeds. The authors of the articles in this issue cover different regions of the world, emphasising the global nature of seed science and the ISTA community.
qualitative study of the experiences of individuals who did not complete the NHS Low Calorie Diet Programme Pilot
Authors: Drew Kevin J , Homer Catherine , Radley Duncan , Bakhai Chirag , Ells Louisa
Published: April 2024

Background: Attrition remains a significant public health challenge as individuals who do not complete programmes are likely to have poorer programme outcomes. On calorie- restricted diets, including the NHS Low Calorie Diet (LCD) Programme pilot, approximately 50% of people are discharged prematurely, and thus do not complete the programme. Reducing attrition therefore has the potential to improve programme efficacy, impact and cost-effectiveness.
Methods: Ten semi-structured interviews were conducted with purposively sampled individuals who did not complete the NHS LCD programme. Interviews explored service user experiences of the programme and experiences of being discharged. Interview data were analysed thematically.
Results: Four core themes were identified: 1) the pre- programme struggles of service users and their route to LCD; 2) a positive and impactful programme; 3) life gets in the way; and 4) a perceived lack of support from the provider. These findings show that individuals had pre-programme struggles and a series of life events that constrained their good intentions, and whilst they were positive about the programme, they were critical of the support they received from providers to deal with their life circumstances.
Conclusions: Policy makers and providers can act proportionately to ensure that programmes, such as the NHS LCD Programme pilot, recognise the circumstances and context of people’s lives, and take a more person-centred approach.
A Chinese survey of clinical practice on the management of thyroid eye disease
Authors: Chen Jingyue , Li Chenyan , Teng Weiping , Shan Zhongyan , Jin Jun , Wei Yining , Sun Jing , Li Yushu , Zhou Huifang
Published: April 2024

Objective

The management of thyroid eye disease (TED) has undergone significant changes for decades. The study sought to investigate current clinical practice on the management of TED in China.

Methods

An online questionnaire survey was conducted from April to May 2023. The questionnaire involved diagnostic criteria for TED, multidisciplinary treatment (MDT) collaboration, and treatment preference for mild, moderate, and severe TED.

Results

A total of 289 questionnaires were collected, with 165 from endocrinologists and 124 from ophthalmologists. Only 36.7% of participants claimed there was an MDT clinical pattern for TED in their institutions. The coverage of biological agents was around 10% or lower. These were distinctly lower than in Western countries. About 62.6% of participants believed the incidence of TED has increased in recent years. Imaging techniques were used widely to assist in the diagnosis of TED. However, there was still controversy regarding the definition of proptosis in the Chinese population. Most doctors managed risk factors and provided orbital supportive treatments of artificial tears and glasses. For mild active TED, endocrinologists (39.4%) were inclined to recommend therapy for hyperthyroidism alone, while ophthalmologists (43.6%) preferred orbital corticosteroid injections. Currently, the most widely used treatment for moderate to severe active TED was high-dose intravenous corticosteroid (94.8%), while orbital radiotherapy combined with immunosuppressive agents was the most recognized second-line therapy (43.6%).

Conclusion

The study documented the consistency and differences between current clinical practices in the management of TED in China and the recently updated guidelines. There was a remarkable difference between ophthalmology and endocrinology departments, warranting management optimization.
Typenatlas der römischen Reichsprägung von Augustus bis Aemilianus, Dritter Band
Authors: Schmidt-Dick Franziska
Published: April 2024

Ziel des Typenatlas der römischen Reichsprägung (TAR) ist die systematische Bearbeitung der Reverstypologie von Münzen der römischen Prinzipatsepoche. Im abschließenden dritten Band des Werkes werden Tiere, Fabeltiere, Pflanzen und Gegenstände untersucht, und zwar nicht nur als Hauptmotiv, sondern auch als Attribut. Die Darbietung geht vom Typ aus und nicht von der Legende, denn diese erklärt oft nicht das Bild. Auf eine Beschreibung jedes Typs folgen Kurzangaben, wann und in welchen Nominalien er ausgeprägt wurde. Daran schließen sich jeweils Tabellen mit den entsprechenden Legenden und Nachweisen an. Auf  86 Tafeln sind alle besprochenen Typen abgebildet. Die neuartige Analyse der Reverstypologie wendet sich nicht nur an Numismatiker:innen, sondern ermöglicht auch den Vertreter:innen der Nachbardisziplinen einen leichteren Zugang zu einer der zentralen zeitgenössischen Primärquellen.
APPROACH TO RESEARCHING BUSINESS ORGANIZATIONS’ COMPETITIVENESS
Authors: Milusheva V.
Published: April 2024

The relevance of the issue presented in the paper stems from the trends in current economic development. They pose multiple challenges to researchers when it comes to methods for assessing and analyzing competitiveness of business organizations. The aim of this paper is to present an approach to researching business organization's competitiveness based on highly versatile and adaptive competitiveness evaluation technology. Part of the methods on which the said approach is based are analysis, summary, logical approach, and includes quantitative assessment of indicators used to make comparisons with competitors. The results of the implementation of this approach by researches are associated with a more precise diagnosis of the causes leading to one level of business organizations’ competitiveness or another. A conclusion can be drawn that the accuracy and effectiveness of any research depends on the preparation of a proper set of methods. When researching business organizations’ competitiveness, it is crucial to take account of the extreme dynamics of the developing business environment.
Does the Outcome of Graft Materials at Dental Implant Sites Differ Between Patients With Normal and Compromised Bone Health?
Authors: Muttanahally Kavya Shankar , Yadav Sumit , Freilich Martin A. , Tadinada Aditya
Published: April 2024

The objective of this paper was to assess the outcome of bone graft material at alveolar bone augmentation sites combined with dental implants in postmenopausal women with compromised bone health by evaluating cone beam computerized tomography (CBCT) scans at multiple time points.CBCT scans were analyzed on 55 postmenopausal women with compromised bone health status to determine the fate of alveolar bone augmentation. CBCT scans were taken immediately after surgery and 9 and 24 months postoperatively. The patient’s medication regimens and durations were recorded, and the pixel intensity value (PIV) was measured and standardized using scoring criteria and visual assessment. Statistical analyses included 2-sample t tests for continuous variables and Fisher’s exact tests for categorical variables.Among the normal patients, 73% received a grade 2 visual score, and 27% received a grade 1 visual score. After 24 months, 45% of patients received a grade 2 score, and 27% received a grade 3 score. In the osteoporotic group receiving medication, 77% of participants received a grade 1 visual score at the 9-month postoperative evaluation, while 23% received a grade 2 score. At the 24-month assessment, 55% of patients received a grade 1 score, 41% received a grade 2 score, and only 5% received a grade 3 score. Notably, although the graft material did not remodel into native bone, it was a scaffold for implants in controlled osteoporotic patients. The study’s results show that the pixel intensity values of particulate graft materials are similar across the three different time points, suggesting that the graft material’s pixel intensity value remains constant in postmenopausal women with osteoporosis. The study’s limitations include a small sample size and a restricted 24-month follow-up period. This limited time frame may need to capture long-term changes or variations in graft materials adequately. Future research should include a larger sample size and have a longer follow-up duration to provide a more comprehensive understanding of the change in graft materials between patients with normal and compromised bone health.
Horizontal Alveolar Ridge Splitting and Expansion
Authors: Flanagan Dennis
Published: April 2024

When considering placing dental implants in atrophic edentulous sites, there may be inadequate site width and little or no vertical bone loss. Any of several surgical procedures can augment these sites. Extracortical augmentation is done by applying graft material against the cortical bone. This technique expects progenitor cells to migrate outside the bony ridge’s confines and form new bone. Another method entails ridge splitting and expansion to create space for osteogenesis and, when possible, implant placement. This may be a better method for horizontal ridge augmentation. The ridge is split, separating the facial and lingual cortices for a complete bone fracture. The patient’s osseous cells can then migrate into the created space from the exposed medullary bone to form bone. The technique can be preferably performed flapless so the intact periosteum maintains a blood supply to ensure appropriate healing.
Perceptions and feelings of pregnant women undergoing outpatient follow-up for toxoplasmosis
Authors:   Universidade Estadual de Maringá, Maringá, Brasil, Nakahara da Silva Viviane Yumi , Vinícius de Lima Lucas ,   Universidade Estadual de Maringá, Maringá, Brasil., Pavinati Gabriel ,   Universidade Estadual de Maringá, Maringá, Brasil, Tavares Magnabosco Gabriela ,   Universidade Estadual de Maringá, Maringá, Brasil, Lopes de Moraes Gil Nelly ,   Universidade Estadual de Maringá, Maringá, Brasil, Machado Cruz Shibukawa Bianca ,   Universidade Federal do Mato Grosso do Sul, Três Lagoas, Brasil.
Published: April 2024

Introduction: Toxoplasmosis persists as a neglected disease and poses a challenge to public health, especially due to the risk of vertical transmission, which can lead to countless biological complications for the newborn and to psychological and emotional repercussions for the mother. Objective: To understand the perceptions and feelings of pregnant women affected by toxoplasmosis undergoing outpatient follow-up. Materials and Methods: A qualitative and exploratory study developed with 12 women with gestational toxoplasmosis undergoing specialized outpatient follow-up in a municipality from the state of Paraná, Brazil. The data were collected through semi-structured individual interviews and subjected to content analysis, supported by descending hierarchical classification. Results: The pregnant women experienced situations ranging from diagnosis and treatment to preventing the disease in the child and family. These experiences generated fear, distress and uncertainty about the disease, which were not adequately addressed during prenatal assistance in primary care. However, the pregnant women emphasized the importance of the multiprofessional team at the secondary level in monitoring and health education. Discussion: Although the pregnant women felt confident about the treatment and its implications for the child's health, discovering the diagnosis impacted their everyday lives and those of their families, especially due to lack of reliable information about toxoplasmosis and to the absence of emotional support at the primary level. Conclusions: There was a temporary scenario of disinformation among these women, who were not properly guided and supported. However, the guidelines offered in secondary health care were essential for improving knowledge and practices in health.
EARLY RESULTS OF SHOULDER ARTHRODESIS WITH 3D-TITANIUM IMPLANTS FOR TREATMENT OF SEVERE GUNSHOT WOUNDS OF THE SHOULDER GIRDLE
Authors: Bondarenko Stanislav , Fomin Oleksandr , Lazarenko Iurii
Published: April 2024

As a result of large-scale war in Ukraine, the frequency of gunshot wounds of the upper extremities has increased dramatically, accompanied by massive damage to soft tissue, neurovascular plexuses, and significant bone deficiency, so their treatment with traditional methods is risky. This leads to the development of new treatment methods, in particular, techniques for shoulder arthrodesis. Objective. To investigate the effectiveness of shoulder arthrodesis using an individual 3D-titanium implant and or a locked compression plate (LCP) with bone autoplasty for the treatment of severe combat trauma of the upper extremity. Methods. In 2022–2023, 19 men aged 36.2 (24–52) years with severe combat trauma of the upper extremity underwent shoulder arthrodesis using individual 3D-titanium implants (n = 9) or LCP with bone autoplasty (n = 10). The follow-up period was 18 months. Individual 3D-implants were created in the CAD program Autodesk Fusion 360 and made of Ti6AI4V alloy by three- dimensional metal 3D-printing. The functional status of the shoulder joint was assessed by the Oxford Shoulder Score, VAS at 6 and 12 months after surgery. Fusion was checked radiographically at 1, 3, 6 and 12 months. Results. The average follow-up period was 12 months. Ankylosis of the shoulder joint was formed in 18 (95 %) patients, and clinical consolidation without final restructuring with a positive tendency to bone fusion was detected in one patient (5 %). Radiologically confirmed fusion in 8.5 months (6–12). After 12 months, a decrease in pain (VAS: 5 to 1 points; p < 0.001) and improvement in the condition of the shoulder (Oxford Shoulder Score: 25 to 40 points, p < 0.001) were found compared with 6 months. Conclusions. Treatment of severe combat trauma of the upper extremity by shoulder arthrodesis allows to eliminate pain and restore sufficient function to perform daily tasks one year after surgery. The combination of shoulder arthrodesis with individual 3D-implants resulted in the restoration of upper limb function in all 9 patients with massive bone and muscle defects.
Proposal of classes for umbu commercialization
Authors: Donato Sérgio Luiz Rodrigues , Castricini Ariane , Rodrigues Maria Geralda Vilela , Deus Joel da Silva de , Arantes Alessandro de Magalhães , Pires Ednei de Souza
Published: April 2024

Abstract: The commercial classification of fruits organizes commercialization and promotes compliance. The aim was to propose classes for umbu fruits based on fresh mass. A total of 4,140 fruits from four harvests of the BRS-68 umbu cultivar (EPAMIG-C01) with average mass of 87.64 g ± 13.99 were used, individually weighed on analytical precision scale, and, the mean and standard deviation of 3,717 fruits within the mean confidence interval were considered for class stratification. Five size classes were determined, medium (= 50 and < 65 g), large (= 65 and < 75 g), giant (= 75 and < 100 g), extra (= 100 and < 110 g) and premium (= 110 g), however, three classes are recommended, large (= 50 and < 75 g), giant (= 75 and < 100 g) and premium (= 100 g), due to their easy adoption and use.
Preparation of Nile tilapia (Oreochromis niloticus) waste meal for human consumption
Authors: Souza Maria Luiza Rodrigues de , Chambo Ana Paula Sartorio , Bronharo Bruna Albino , Vasconcellos Ricardo Souza , Khatlab Angélica de Souza , Matiucci Marcos Antonio , Gasparino Eliane
Published: April 2024

ABSTRACT: This study aimed to prepare and characterize Nile tilapia (Oreochromis niloticus) waste meal. The experimental design consisted of three treatments (carcass meal, mechanically separated meat meal - MSM, and head meal) and five replications of each treatment. Yield, microbiological quality, pH, water activity, proximate composition, energy value, mineral composition, fatty acid profile, amino acid composition, color, and particle size fractions and the geometric mean particle diameter (GMD) analyses were performed. Data were subjected to ANOVA, and means were compared by Tukey’s test at P < 0.05. Carcass, mechanically separated meat, and head meals had a protein content of 56.45%, 78.60%, and 50.33%, respectively, a lipid content of 7.16%, 13.15%, and 4.58%, respectively, and an ash content of 32.61%, 4.26%, and 38.41%, respectively. The mean yield of fish waste meals was 13%. Oleic, palmitic, and linoleic acids were the major fatty acids in the three fish waste meals, and glutamic acid, leucine, lysine, and glycine were the major amino acids. Color analysis showed that lightness (L*) ranged from 55.32 to 77.19, redness (a*) from 4.44 to 4.88, and yellowness (b*) from 4.13 to 9.51. The GMD ranged from 0.37 to 0.99 mm. The results indicate that Nile tilapia waste meals have high nutritional value and adequate pH, water activity, and microbiological quality for use in human nutrition.
Antifungal activity and mechanism of action of monoterpenes against Botrytis cinerea
Authors: Pedroso Marília Brandão , Scariot Fernando Joel , Rocha Ronaldo Kaue Mattos , Echeverrigaray Sergio , Delamare Ana Paula Longaray
Published: April 2024

ABSTRACT Botrytis cinerea is considered one of the most important post-harvest pathogens being the causative agent of gray rot. To reduce the use of synthetic fungicides, it is important to explore alternative products with antifungal properties. Among these alternative products are essential oils, which present monoterpenes as major compounds. The objective of this study was to evaluate the effects of eight monoterpenes (1,8-cineole, carvacrol, citral, citronellal, citronellol, geraniol, linalool, and thymol) on the control of B. cinerea. The mycelial growth of B. cinerea was assessed after treating it with the monoterpenes at a concentration of 1,000 mg/L. Subsequently, the minimal inhibitory concentrations (IC90) of the monoterpenes that showed the greatest antifungal potential were determined. Carvacrol and thymol were tested on B. cinerea cell membrane integrity, intracellular accumulation of reactive oxygen species (ROS), and mitochondrial membrane potential of the conidia. Among the tested monoterpenes carvacrol, citral, citronellal, citronellol, geraniol, and thymol demonstrated complete inhibition of mycelial growth at a concentration of 1,000 mg/L. Carvacrol and thymol exhibited the lowest IC90 values against B. cinerea, with an IC90 of 125 mg/L. Furthermore, carvacrol and thymol induced conidial death in a dose-dependent manner, resulting in the disruption of cell membrane integrity, increased intracellular ROS levels, and decreased mitochondrial membrane potential. These findings highlight the potential of carvacrol and thymol as alternative means of controlling B. cinerea.
A intencionalidade da ação docente em aulas de Ciências do Ensino Fundamental
Authors: Arruda Sérgio de Mello , Meneguete Hemilyn da Silva , Passos Marinez Meneghello , Turke Nathália Hernandes
Published: April 2024

Resumo: O presente artigo descreve uma investigação sobre a intencionalidade da ação docente em aulas de Ciências do Ensino Fundamental. Assumindo que a intencionalidade tem duas componentes, plano de ação e objetivo, analisamos três episódios de uma aula de Ciências, ministrada por uma professora, sobre o conteúdo rochas, do sexto ano do Ensino Fundamental. Os dados demonstram que o objetivo geral nos três episódios correspondeu a uma abordagem metodológica diversificada que propunha: (i) descobrir como pensam os alunos, (ii) expor o conteúdo e (iii) realizar um experimento demonstrativo que auxiliaria na compreensão do conteúdo. O Plano Geral de Ação para os mesmos episódios, que consideramos compatível com seu Objetivo Geral, envolveu: fazer perguntas gerais e específicas; procurar entender as ideias dos alunos; gerenciar a ação dos alunos; explicar o conteúdo e o experimento; escrever o conteúdo na lousa; realizar um experimento demonstrativo.
Atendimento às vítimas de violência no serviço de emergência na perspectiva da enfermagem forense
Authors: Citolin Morgana Oliveira , Vargas Mara Ambrosina de Oliveira , Santos Davydson Gouveia , Hilleshein Andreza Goulart , Brasil Gisele , Ramos Flavia Regina Souza
Published: April 2024

Objetivo: analisar a atuação dos enfermeiros no recolhimento, identificação e preservação de vestígios no atendimento de emergência à vítima de violência, na perspectiva desses profissionais. Método: estudo qualitativo, com abordagem descritiva exploratória. Desenvolvido por meio de entrevista semiestruturada com 21 enfermeiros de hospitais que integram o fluxo intersetorial para atendimentos às vítimas de violência de dois hospitais de referência neste atendimento, em uma capital do sul do Brasil. Foram incluídos enfermeiros membros da equipe multiprofissional que atuam na emergência nos respectivos hospitais, e o critério de exclusão foi profissionais realocados na emergência durante a pandemia. A análise de dados foi realizada conforme análise de conteúdo temático. Resultados: os dados foram discutidos em cinco categorias: 1) Qualificação Profissional; 2) Protocolo Institucional e Materiais; 3) Percepções dos profissionais; 4) Ações dos profissionais e 5) Estrutura da equipe. Conclusão: as competências dos profissionais de enfermagem no recolhimento, identificação e preservação de vestígios no atendimento de emergência à vítima de violência precisam ser melhor organizadas, estruturadas e padronizadas. É incontestável a presença de profissionais enfermeiros nos atendimentos às vítimas de violência nos serviços de emergência, mas sua importância ainda é subestimada e sua potencial contribuição para a abordagem pericial é subutilizada.
Avaliação de diferentes métodos de mensuração da temperatura corporal de pacientes no período intraoperatório
Authors: Nascimento Ariane Souza do , Lemos Cassiane de Santana , Biachi Fernanda Baratojo , Lyra Fernanda Ribeiro Silva de , Gnatta Juliana Rizzo , Poveda Vanessa de Brito
Published: April 2024

Objetivos: este estudo teve como objetivo estimar e comparar a confiabilidade das medições de temperatura obtidas com um termômetro temporal infravermelho periférico, um termômetro cutâneo central (“ Zero-Heat-Flux ”) e um termômetro esofágico ou nasofaríngeo entre pacientes cirúrgicos eletivos no período intraoperatório. Método: estudo longitudinal com medidas repetidas realizado por amostragem de conveniência de 99 pacientes, com 18 anos ou mais, submetidos a cirurgia eletiva de câncer abdominal, com duração de anestesia de pelo menos uma hora, com cada paciente tendo sua temperatura medida pelos três métodos. Resultados: o coeficiente de correlação intraclasse mostrou uma baixa correlação entre as medições usando o termômetro temporal periférico e os termômetros cutâneo central (0,0324) e esofágico/nasofaríngeo (-0,138). Houve uma alta correlação (0,744) entre os termômetros centrais avaliados. Conclusão: os dados do presente estudo não recomendam o uso de um termômetro infravermelho temporal como estratégia para medir a temperatura corporal de pacientes submetidos a procedimentos anestésico-cirúrgicos. O termômetro cutâneo central e o termômetro esofágico/nasofaríngeo são equivalentes para detectar hipotermia intraoperatória.
Streptococcus lutetiensis and Streptococcus equinus as potential emerging bovine mastitis pathogens
Authors: Crippa Bruna L. , Rodrigues Marjory X. , Tomazi Tiago , Bicalho Rodrigo C. , Silva Nathália C.C.
Published: April 2024

ABSTRACT: The current study characterizes the genetic distribution of virulence and antimicrobial resistance of Streptococcus lutetiensis and Streptococcus equinus isolated from cows with clinical mastitis using whole genome sequencing (WGS). Although they are not the protagonist species within the genus Streptococcus, recent studies have isolated these species associated with bovine mastitis. In addition, these species are reported and isolated from humans and other animals. A total of four strains of S. lutetiensis and one of S. equinus were isolated from five cows with identified cases of clinical mastitis at a dairy farm near Ithaca, New York. Nineteen genes associated with antimicrobial resistance and 20 genes associated with virulence were identified in the analyzed strains. All strains presented genes associated with resistance: alr, ddl, gdpD, kasA, murA, lsa(E), msr(D), mef(A), gidB, and LiaF. Resistance genes associated with several different classes of antibiotics have also been reported. Sixteen virulence-associated genes were identified in all strains. Based on our findings, we conclude that the studied species have the potential to cause mastitis in cattle, and further studies are important to elucidate their role.