bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring temporary urban interventions through user activities: a case study of Abdali Boulevard, Amman, Jordan
Authors: Sharif Ahlam Ammar
Published: May 2023

Introduction: Urban studies have examined the potential of urban interventions to upgrade spaces. This is the focus of relational approaches that emphasize interactions between interventions and users. One approach, actor–network theory, views these interactions as temporary stabilized relational effects. Despite its usefulness in uncovering diverse interactions in urban spaces, the utilization of actor–network theory in unpacking these relations has been limited.

Methods: This study utilized an actor–network theory-inspired ethnography in a temporary urban intervention in Abdali Boulevard, in Jordan, to bridge this gap. It relied on desk research, interviews, and site observations to explore the different intervention–user interactions.

Results: The findings revealed various interactions and relational patterns occurring between interventions and their users through their presence and absence in space and time, where users attended to, engaged with, overlooked, bypassed, disturbed the intervention, or floated between different modes of interaction.

Discussion: Unpacking these diverse interactions and relations provides a nuanced perspective on the effects of urban interventions on spaces. This would be useful for designers in developing new ways of designing through offering interventions that engage the public.
Cartilage regeneration in zebrafish depends on Nrg1/ErbB signaling pathway
Authors: Sapède Dora , Bahraoui Sarah , Abou Nassif Léa , Barthelaix Audrey , Mathieu Marc , Jorgensen Christian , Djouad Farida
Published: May 2023

Objective: Cartilage, as the majority of adult mammalian tissues, has limited regeneration capacity. Cartilage degradation consecutive to joint injury or aging then leads to irreversible joint damage and diseases. In contrast, several vertebrate species such as the zebrafish have the remarkable capacity to spontaneously regenerate skeletal structures after severe injuries. The objective of our study was to test the regenerative capacity of Meckel’s cartilage (MC) upon mechanical injury in zebrafish and to identify the mechanisms underlying this process.

Methods and Results: Cartilage regenerative capacity in zebrafish larvae was investigated after mechanical injuries of the lower jaw MC in TgBAC(col2a1a:mCherry), to visualize the loss and recovery of cartilage. Confocal analysis revealed the formation of new chondrocytes and complete regeneration of MC at 14 days post-injury (dpi) via chondrocyte cell cycle re-entry and proliferation of pre-existing MC chondrocytes near the wound. Through expression analyses, we showed an increase of nrg1 expression in the regenerating lower jaw, which also expresses Nrg1 receptors, ErbB3 and ErbB2. Pharmacological inhibition of the ErbB pathway and specific knockdown of Nrg1 affected MC regeneration indicating the pivotal role of this pathway for cartilage regeneration. Finally, addition of exogenous NRG1 in an in vitro model of osteoarthritic (OA)-like chondrocytes induced by IL1β suggests that Nrg1/ErbB pathway is functional in mammalian chondrocytes and alleviates the increased expression of catabolic markers characteristic of OA-like chondrocytes.

Conclusion: Our results show that the Nrg1/ErbB pathway is required for spontaneous cartilage regeneration in zebrafish and is of interest to design new therapeutic approaches to promote cartilage regeneration in mammals.
Virtual monitoring in CF – the importance of continuous monitoring in a multi-organ chronic condition
Authors: Vagg Tamara , Deasy Kevin F. , Chapman Wendy W. , Ranganathan Sarath C. , Plant Barry J. , Shanthikumar Shivanthan
Published: May 2023

Cystic Fibrosis (CF) is a chronic life-limiting condition that affects multiple organs within the body. Patients must adhere to strict medication regimens, physiotherapy, diet, and attend regular clinic appointments to manage their condition effectively. This necessary but burdensome requirement has prompted investigations into how different digital health technologies can enhance current care by providing the opportunity to virtually monitor patients. This review explores how virtual monitoring has been harnessed for assessment or performance of physiotherapy/exercise, diet/nutrition, symptom monitoring, medication adherence, and wellbeing/mental-health in people with CF. This review will also briefly discuss the potential future of CF virtual monitoring and some common barriers to its current adoption and implementation within CF. Due to the multifaceted nature of CF, it is anticipated that this review will be relevant to not only the CF community, but also those investigating and developing digital health solutions for the management of other chronic diseases.
Gender-specific effects of oxidative balance score on the prevalence of diabetes in the US population from NHANES
Authors: Wu Cuiling , Ren Chenxia , Song Yingda , Gao Huifang , Pang Xin , Zhang Lianyun
Published: May 2023

Background

The relationship between oxidative balance score (OBS) and diabetes remains poorly understood and may be gender-specific. We conducted a cross-sectional study to investigate the complex association between OBS and diabetes among US adults.

Methods

Overall, 5,233 participants were included in this cross-sectional study. The exposure variable was OBS, composed of scores for 20 dietary and lifestyle factors. Multivariable logistic regression, subgroup analysis, and restricted cubic spline (RCS) regression were applied to examine the relationship between OBS and diabetes.

Results

Compared to the lowest OBS quartile group (Q1), the multivariable-adjusted odds ratio (OR) (95% confidence interval (CI) for the highest OBS quartile group (Q4) was 0.602 (0.372–0.974) (p for trend = 0.007), and for the highest lifestyle, the OBS quartile group was 0.386 (0.223–0.667) (p for trend < 0.001). Moreover, gender effects were found between OBS and diabetes (p for interaction = 0.044). RCS showed an inverted-U relationship between OBS and diabetes in women (p for non-linear = 6e−04) and a linear relationship between OBS and diabetes in men.

Conclusions

In summary, high OBS was negatively associated with diabetes risk in a gender-dependent manner.
Salvage decision-making based on carbon following an eastern spruce budworm outbreak
Authors: Scott Lisa N. , Smith Sean M. , Gunn John S. , Petrik Marek , Ducey Mark J. , Buchholz Thomas S. , Belair Ethan P.
Published: May 2023

Forest disturbances, such as an eastern spruce budworm (Choristoneura fumiferana) outbreak, impact the strength and persistence of forest carbon sinks. Salvage harvests are a typical management response to widespread tree mortality, but the decision to salvage mortality has large implications for the fate of carbon stocks (including forest carbon and harvested wood products) in the near and long terms. In this study, we created decision-support models for salvage harvesting based on carbon after an eastern spruce budworm outbreak. We used lasso regression to determine which stand characteristics (e.g., basal area) are the best predictors of carbon 40 years after an outbreak in both salvage and no salvage scenarios. We modeled carbon at year 40 for different treatment scenarios and discount rates. Treatment scenarios represent residual stand conditions that may be present when an outbreak occurs. Economic discount rates were applied to 40-year carbon values to account for near and long-term carbon storage aspects. We found that the volume and size of eastern spruce budworm host species are significant predictors of salvage preference based on carbon. We found overall that salvaging less volume is recommended to avoid major swings in carbon budgets and that discounting carbon values to apply weight to near or long-term sequestration greatly affects whether salvaging is preferred. Lasso models are constructed for the northeastern US, however, similar concepts may be applied beyond our study area and potentially for other insect outbreaks similar to spruce budworm, such as mountain pine beetle (Dendroctonus ponderosae) or hemlock woolly adelgid (Adelges tsugae). From a policy standpoint widespread salvaging could create a large carbon emissions deficit with the risk of not being fully replenished within a desired timeframe. Since salvaging is often financially driven, especially for private landowners, carbon market payments or incentives for not salvaging is a consideration for future policy.
Remission of social behavior impairment by oral administration of a precursor of NAD in CD157, but not in CD38, knockout mice
Authors: Gerasimenko Maria , Higashida Haruhiro
Published: May 2023

Nicotinamide adenine dinucleotide (NAD) is a substrate of adenosine diphosphate (ADP)-ribosyl cyclase and is catalyzed to cyclic ADP-ribose (cADPR) by CD38 and/or CD157. cADPR, a Ca2+ mobilizing second messenger, is critical in releasing oxytocin from the hypothalamus into the brain. Although NAD precursors effectively play a role in neurodegenerative disorders, muscular dystrophy, and senescence, the beneficial effects of elevating NAD by NAD precursor supplementation on brain function, especially social interaction, and whether CD38 is required in this response, has not been intensely studied. Here, we report that oral gavage administration of nicotinamide riboside, a perspective NAD precursor with high bioavailability, for 12 days did not show any suppressive or increasing effects on sociability (mouse’s interest in social targets compared to non-social targets) in both CD157KO and CD38KO male mice models in a three-chamber test. CD157KO and CD38KO mice displayed no social preference (that is, more interest towards a novel mouse than a familiar one) behavior. This defect was rescued after oral gavage administration of nicotinamide riboside for 12 days in CD157KO mice, but not in CD38KO mice. Social memory was not observed in CD157KO and CD38KO mice; subsequently, nicotinamide riboside administration had no effect on social memory. Together with the results that nicotinamide riboside had essentially no or little effect on body weight during treatment in CD157KO mice, nicotinamide riboside is less harmful and has beneficial effect on defects in recovery from social behavioral, for which CD38 is required in mice.
Multi-scale and cross-dimensional TMS mapping: A proof of principle in patients with Parkinson’s disease and deep brain stimulation
Authors: Passera Brice , Harquel Sylvain , Chauvin Alan , Gérard Pauline , Lai Lisa , Moro Elena , Meoni Sara , Fraix Valerie , David Olivier , Raffin Estelle
Published: May 2023

Introduction

Transcranial magnetic stimulation (TMS) mapping has become a critical tool for exploratory studies of the human corticomotor (M1) organization. Here, we propose to gather existing cutting-edge TMS-EMG and TMS-EEG approaches into a combined multi-dimensional TMS mapping that considers local and whole-brain excitability changes as well as state and time-specific changes in cortical activity. We applied this multi-dimensional TMS mapping approach to patients with Parkinson’s disease (PD) with Deep brain stimulation (DBS) of the sub-thalamic nucleus (STN) ON and OFF. Our goal was to identifying one or several TMS mapping-derived markers that could provide unprecedent new insights onto the mechanisms of DBS in movement disorders.

Methods

Six PD patients (1 female, mean age: 62.5 yo [59–65]) implanted with DBS-STN for 1 year, underwent a robotized sulcus-shaped TMS motor mapping to measure changes in muscle-specific corticomotor representations and a movement initiation task to probe state-dependent modulations of corticospinal excitability in the ON (using clinically relevant DBS parameters) and OFF DBS states. Cortical excitability and evoked dynamics of three cortical areas involved in the neural control of voluntary movements (M1, pre-supplementary motor area – preSMA and inferior frontal gyrus – IFG) were then mapped using TMS-EEG coupling in the ON and OFF state. Lastly, we investigated the timing and nature of the STN-to-M1 inputs using a paired pulse DBS-TMS-EEG protocol.

Results

In our sample of patients, DBS appeared to induce fast within-area somatotopic re-arrangements of motor finger representations in M1, as revealed by mediolateral shifts of corticomuscle representations. STN-DBS improved reaction times while up-regulating corticospinal excitability, especially during endogenous motor preparation. Evoked dynamics revealed marked increases in inhibitory circuits in the IFG and M1 with DBS ON. Finally, inhibitory conditioning effects of STN single pulses on corticomotor activity were found at timings relevant for the activation of inhibitory GABAergic receptors (4 and 20 ms).

Conclusion

Taken together, these results suggest a predominant role of some markers in explaining beneficial DBS effects, such as a context-dependent modulation of corticospinal excitability and the recruitment of distinct inhibitory circuits, involving long-range projections from higher level motor centers and local GABAergic neuronal populations. These combined measures might help to identify discriminative features of DBS mechanisms towards deep clinical phenotyping of DBS effects in Parkinson’s Disease and in other pathological conditions.
The acute adaptation of skin microcirculatory perfusion in vivo does not involve a local response but rather a centrally mediated adaptive reflex
Authors: Monteiro Rodrigues Luís , Rocha Clemente , Andrade Sérgio , Granja Tiago , Gregório João
Published: May 2023

Introduction: Cardiovascular homeostasis involves the interaction of multiple players to ensure a permanent adaptation to each organ’s needs. Our previous research suggested that changes in skin microcirculation—even if slight and distal—always evoke an immediate global rather than “local” response affecting hemodynamic homeostasis. These observations question our understanding of known reflexes used to explore vascular physiology, such as reactive hyperemia and the venoarteriolar reflex (VAR). Thus, our study was designed to further explore these responses in older healthy adults of both sexes and to potentially provide objective evidence of a centrally mediated mechanism governing each of these adaptive processes.

Methods: Participants (n = 22, 52.5 ± 6.2 years old) of both sexes were previously selected. Perfusion was recorded in both feet by laser Doppler flowmetry (LDF) and photoplethysmography (PPG). Two different maneuvers with opposite impacts on perfusion were applied as challengers to single limb reactive hyperemia evoked by massage and a single leg pending to generate a VAR. Measurements were taken at baseline (Phase I), during challenge (Phase II), and recovery (Phase III). A 95% confidence level was adopted. As proof of concept, six additional young healthy women were selected to provide video imaging by using optoacoustic tomography (OAT) of suprasystolic post-occlusive reactive hyperemia (PORH) in the upper limb.

Results: Modified perfusion was detected by LDF and PPG in both limbs with both hyperemia and VAR, with clear systemic hemodynamic changes in all participants. Comparison with data obtained under the same conditions in a younger cohort, previously published by our group, revealed that results were not statistically different between the groups.

Discussion: The OAT documentary and analysis showed that the suprasystolic pressure in the arm changed vasomotion in the forearm, displacing blood from the superficial to the deeper plexus vessels. Deflation allowed the blood to return and to be distributed in both plexuses. These responses were present in all individuals independent of their age. They appeared to be determined by the need to re-establish hemodynamics acutely modified by the challenger, which means that they were centrally mediated. Therefore, a new mechanistic interpretation of these exploratory maneuvers is required to better characterize in vivo cardiovascular physiology in humans.
Toward consistent reporting of sample characteristics in studies investigating the biological mechanisms of romantic love
Authors: Bode Adam , Kowal Marta
Published: May 2023

In this non-systematic review, we consider the sample reporting practices of 42 studies up to and including 2021 investigating the biological mechanisms of romantic love (i.e., 31 neuroimaging studies, nine endocrinological studies, one genetics study, and one combined neuroimaging and genetics study). We searched scientific databases using key terms and drew on our and other authors’ knowledge to identify studies that investigated the mechanisms associated with romantic love using neuroimaging, endocrinological, and genetic methods. Only studies with a group or entire sample experiencing romantic love were included. The aim was to collate all relevant studies and determine the comparability of studies and ability to assess the generalizability of findings. We summarize how these studies report sex/gender, age, romantic love, relationship duration/time in love, and sample descriptors. We then outline the case for promoting comparability and the ability to determine generalizability in future studies. The findings indicate a limited ability to compare studies’ samples or make an assessment of the generalizability of findings. Existing studies are not representative of the general population in a particular country or globally. We conclude by presenting ideas about how best to report sex, age, romantic love characteristics, relationship status, time in love, relationship duration, relationship satisfaction, type of unrequited love, sexual activity, cultural characteristics, socio-economic status, student status, and method-relevant descriptors. If our ideas are adopted, in part or in whole, we expect the comparability of studies to increase. Adopting our ideas will also make it easier to make an assessment of the generalizability of findings.
Non-violent resistance parental training versus treatment as usual for children and adolescents with severe tyrannical behavior: a randomized controlled trial
Authors: Fongaro Erica , Aouinti Safa , Picot Marie-Christine , Pupier Florence , Omer Haim , Franc Nathalie , Purper-Ouakil Diane
Published: May 2023

Objective

This single-blinded, randomized, parallel group superiority trial evaluates whether the Non-Violent Resistance (NVR) program, a 10-session parental-group intervention, was more effective in reducing stress in parents of children aged 6–20 years and displaying severe tyrannical behavior (STB) compared to a treatment as usual (TAU) intervention that provided supportive counseling and psychoeducation.

Methods

Eighty two parents of youth aged 6–20 years with STB were enrolled by the Child and Adolescent Psychiatry Department at the University Hospital of Montpellier (France). A random block and stratified by age (6–12 and 13–20 years) randomization, was performed. All participants were interviewed by independent, blinded to group assignments, research assistants, and completed their assessments at baseline and treatment completion (4 months from baseline). Since this program has not been previously evaluated in this population, the study primarily evaluated the efficacy, using the Parenting Stress Index/Short Form (PSI-SF). The primary outcome was the change from baseline to treatment completion of the PSI-SF total score.

Results

Seventy three participants completed the study and were available for analysis (36 NVR and 37 TAU). At completion, between-groups comparison of the change (completion minus baseline) in the total score of PSI-SF was not significant (NVR: −4.3 (± 13.9); TAU: −7.6 (± 19.6); two-sample t-test p = 0.43; effect size of −0.19 [−0.67, 0.28]).

Conclusion

Contrary to our expectation, NVR was not superior to TAU in reducing parental stress at completion for parents of children with STB. However, NVR showed positive outcomes in the follow-up, pointing to the importance to implement parental strategies and following this population over longer time periods in future projects.

Clinical trial registration: Clinicaltrials.gov, identifier NCT05567276.
Unlocking asylum seekers’ voices: protocol of a mixed-method clinical study on the use of the cultural formulation interview with asylum seekers in Belgium
Authors: Claus Lukas , Schouler-Ocak Meryam , Braakman Mario H. , Sabbe Bernard , Van Beuren Godfried , van den Ameele Seline
Published: May 2023

Background

Despite a high prevalence of mental disorders among asylum seekers, many barriers to mental healthcare exist. Cultural and contextual factors strongly influence the experience and expression of psychological distress, putting asylum seekers at greater risk of misdiagnosis and inappropriate treatment. The Cultural Formulation Interview (CFI) is a useful tool to map out cultural and contextual factors of mental disorders; however, to the best of our knowledge, it has not yet been investigated in asylum seekers specifically. The primary aim of this study is to evaluate the value of the CFI in the psychiatric assessment of asylum seekers. Second, we will describe the themes relevant to psychiatric distress in asylum seekers that are identified by the CFI. In addition, asylum seekers’ experience of the CFI will be evaluated.

Methods and analysis

This cross-sectional, mixed-method clinical study aims to recruit a group of 60–80 asylum seekers (age 15–29) with mental health symptoms. Data will be collected using structured (MINI, PCL-5, HDRS-17, WHOQoL-BREF & BSI) and semi-structured (CFI & CFI-debriefing) questionnaires to assess cultural background, contextual factors, and illness severity. Multidisciplinary case discussions will be held after the completion of interviews, following a methodological stepped approach. Combining qualitative and quantitative research techniques, this study aims to generate reliable knowledge on working with the CFI in asylum seekers. Based on the findings, recommendations for clinicians will be developed.

Discussion

This study addresses the knowledge gap on using the CFI in asylum seekers. Compared to prior studies, it will provide new insights into the use of the CFI in the specific context of working with asylum seekers.

Ethics and dissemination

Prior research on the CFI in asylum seekers is limited, partly because of their high vulnerability and low access to care. The study protocol has been tailored in close collaboration with several stakeholders and validated after piloting. Ethical approval has already been obtained. Together with the stakeholders, the results will be translated into guidelines and training materials. Recommendations to policymakers will also be provided.
Relationship between birth weight and ambient temperature during pregnancy in a cross-sectional study of the residents of Suzhou, China
Authors: Ding Yi , Zhou Hui , Tong Min , Chen Xiaofang , Zhao Qian , Ma Yuqin , Wu Lei
Published: May 2023

Objective

The association between birth weight and ambient temperature during pregnancy remains inconclusive, and data from Chinese populations are scarce. We conducted a cross-sectional study to investigate the association between birth weight and ambient temperature during pregnancy among the residents of Suzhou Industrial Park, Suzhou, China.

Methods

Information regarding 10,903 infants born between January 2018 and December 2018 who were born at the hospitals in Suzhou Industrial Park, Jiangsu province was obtained via public birth records.

Results

This study found that the ambient temperature during the first trimester of pregnancy was negatively correlated with birth weight, suggesting that elevated temperature may be related to lower birth weight. However, the ambient temperatures during the second and third trimesters of pregnancy were positively correlated with birth weight. Moreover, when the ambient temperature was below 15°C during the second trimester of pregnancy, the birth weight increased with temperature. However, when the temperature was higher than 15°C, the birth weight decreased with temperature. The relationship between ambient temperature in the third trimester and birth weight presented an inverted “U” curve. When the ambient temperature was lower than 20°C, the birth weight increased with ambient temperature, but when the ambient temperature was higher than 20°C, the increase of ambient temperature showed no significant relationship with the increase of birth weight.

Conclusion

The ambient temperature was correlated with birth weight. The ambient temperature during the first trimester of pregnancy was negatively correlated with birth weight. The relationship between ambient temperature in the third trimester and birth weight presented an inverted “U” curve.
Knowledge, attitude, and practice of menstrual blood-derived mesenchymal stem cells among female healthcare workers in India
Authors: Kaur Karuna Nidhi , Nandi Dhruva , Ramachandran Krithika , Mohanan Lita , Subhashini S. , Segan Mehak , Tripathy Saswaty , Janardhanan Rajiv
Published: May 2023

Introduction

Mesenchymal stem cells (MSCs) are pluripotent progenitor cells that can be differentiated into a variety of specialized cell types. Menstrual blood, such as umbilical cord blood and bone marrow stem cells, is a rich source of MSCs with proliferative properties. This research was conducted to understand the knowledge, attitude, and practice of menstrual blood donation regarding menstrual blood-derived mesenchymal stem cells (MenSCs) among female healthcare workers in India.

Methodology

A cross-sectional online and offline survey was conducted at the national level between 20 November 2021 and 10 March 2022. A self-constructed semi-structured questionnaire was distributed through Google Forms on various social media platforms. The questionnaire was self-administered, and data were collected using the purposive sampling technique.

Results

A total of 499 respondents completed the questionnaire. Nearly 49% of the respondents had adequate knowledge, 54% showed a positive attitude, and 45% reported adequate practices regarding menstrual blood donation and the usage of related products. The educational background, occupational status, and monthly income of the participants were found to be significantly associated with their attitude toward MenSCs.

Conclusion

There is a need to promote interactive sessions on MenSCs among healthcare professionals in order to bridge the gap between general populations and the healthcare setting. Enhancing knowledge and awareness regarding the potential benefits of MenSCs would help in dissipating the age-old myths associated with menstruation and will benefit society as a whole.
Associations of psychological wellbeing with COVID-19 hospitalization and mortality in adults aged 50 years or older from 25 European countries and Israel
Authors: Wang Wenjun , Wang Jingjing , Shi Juanjuan , Li Yaping , Zhang Xin , Wu Fengping , Wang Yikai , Li Jia , Hao Miao , Liu Xiongtao , Zhai Song , Wang Yuan , Gao Ning , Tian Yan , Lu Rui , Yeo Yee Hui , Jia Xiaoli , Ji Fanpu , Dang Shuangsuo
Published: May 2023

Background

Lower psychological wellbeing is associated with poor outcomes in a variety of diseases and healthy populations. However, no study has investigated whether psychological wellbeing is associated with the outcomes of COVID-19. This study aimed to determine whether individuals with lower psychological wellbeing are more at risk for poor outcomes of COVID-19.

Methods

Data were from the Survey of Health, Aging, and Retirement in Europe (SHARE) in 2017 and SHARE's two COVID-19 surveys in June–September 2020 and June–August 2021. Psychological wellbeing was measured using the CASP-12 scale in 2017. The associations of the CASP-12 score with COVID-19 hospitalization and mortality were assessed using logistic models adjusted for age, sex, body mass index, smoking, physical activity, household income, education level, and chronic conditions. Sensitivity analyses were performed by imputing missing data or excluding cases whose diagnosis of COVID-19 was solely based on symptoms. A confirmatory analysis was conducted using data from the English Longitudinal Study of Aging (ELSA). Data analysis took place in October 2022.

Results

In total, 3,886 individuals of 50 years of age or older with COVID-19 were included from 25 European countries and Israel, with 580 hospitalized (14.9%) and 100 deaths (2.6%). Compared with individuals in tertile 3 (highest) of the CASP-12 score, the adjusted odds ratios (ORs) of COVID-19 hospitalization were 1.81 (95% CI, 1.41–2.31) for those in tertile 1 (lowest) and 1.37 (95% CI, 1.07–1.75) for those in tertile 2. As for COVID-19 mortality, the adjusted ORs were 2.05 (95% CI, 1.12–3.77) for tertile 1 and 1.78 (95% CI, 0.98–3.23) for tertile 2, compared with tertile 3. The results were relatively robust to missing data or the exclusion of cases solely based on symptoms. This inverse association of the CASP-12 score with COVID-19 hospitalization risk was also observed in ELSA.

Conclusion

This study shows that lower psychological wellbeing is independently associated with increased risks of COVID-19 hospitalization and mortality in European adults aged 50 years or older. Further study is needed to validate these associations in recent and future waves of the COVID-19 pandemic and other populations.
Type 2 diabetes and the risk of synovitis-tenosynovitis: a two-sample Mendelian randomization study
Authors: Guo Jiale , Peng Caiju , He Qionghan , Li Yehai
Published: May 2023

Introduction

It has been shown that people with type 2 diabetes have a higher risk of synovitis and tenosynovitis, but previous studies were mainly observational, which may be biased and does not allow for a cause-and-effect relationship. Therefore, we conducted a two-sample Mendelian randomization (MR) study to investigate the causal relationship.

Method

We obtained data on “type 2 diabetes” and “synovitis, tenosynovitis” from published large-scale genome-wide association studies (GWAS). The data were obtained from the FinnGen consortium and UK Biobank, both from European population samples. We used three methods to perform a two-sample MR analysis and also performed sensitivity analysis.

Results

The results of all three MR methods we used for the analysis illustrated that T2DM increases the risk factor for the development of synovitis and tenosynovitis. Specifically, for the IVW method as the primary analysis outcome, OR = 1.0015 (95% CI, 1.0005 to 1.0026), P = 0.0047; for the MR Egger method as the supplementary analysis outcome, OR = 1.0032 (95% CI, 1.0007 to 1.0056), P = 0.0161; for the weighted median method, OR = 1.0022 (95% CI, 1.0008 to 1.0037), p = 0.0018. In addition, the results of our sensitivity analysis suggest the absence of heterogeneity and pleiotropy in our MR analysis.

Conclusion

In conclusion, the results of our MR analysis suggest that T2DM is an independent risk factor for increased synovitis and tenosynovitis.
Incidence, knowledge, attitude and practice toward needle stick injury among nursing students in Saudi Arabia
Authors: Al-Mugheed Khalid , Farghaly Sally Mohammed , Baghdadi Nadiah A. , Oweidat Islam , Alzoubi Majdi M.
Published: May 2023

Background

Needle stick injuries constitute the greatest threat to nursing students during clinical practice because of accidental exposure to body fluids and infected blood. The purpose of this study was to (1) determine the prevalence of needle stick injuries and (2) measure the level of knowledge, attitude and practice among nursing students about needle stick injuries.

Methods

Three hundred participants undergraduate nursing students at a private college in Saudi Arabia were included, of whom 281 participated, for an effective response rate of 82%.

Results

The participants showed good knowledge scores with a mean score of 6.4 (SD = 1.4), and results showed that students had positive attitudes (Mean = 27.1, SD = 4.12). Students reported a low level of needle stick practice (Mean = 14.1, SD = 2.0). The total prevalence of needle stick injuries in the sample was 14.1%. The majority, 65.1%, reported one incidence in the last year, while (24.4%) 15 students reported two incident of needle stick injuries. Recapping was the most prevalent (74.1%), followed by during injection (22.3%). Most students did not write a report (77.4%), and being worried and afraid were the main reasons for non-reports (91.2%). The results showed that female students and seniors scored higher level in all needle stick injuries domains (knowledge, attitude and practice) than male students and juniors. Students who had needle stick injuries more than three times last year reported a lower level of all needle stick injury domains than other groups (Mean = 1.5, SD =1.1; Mean = 19.5, SD =1.1; Mean = 9.5, SD =1.1, respectively).

Conclusion

Although the student’s showed good knowledge and positive attitudes in NSI, the students reported a low level of needle stick practice. Raising awareness among nursing students and conducting continuing education related to sharp devices and safety and how to write an incident reporting is highly recommended.
Impact of sudden stratospheric warmings on the neutral density, temperature and wind in the MLT region
Authors: Zhou Baozhu , Yi Wen , Xue Xianghui , Ye Hailun , Zeng Jie , Li Guozhu , Tsutsumi Masaki , Gulbrandsen Njål , Chen Tingdi , Dou Xiankang
Published: May 2023

In this study, the neutral density and horizontal wind observed by the four meteor radars, as well as the temperature measured by the Microwave Limb Sounder (MLS) onboard the Aura satellite are used to examine the response of neutral density, wind, and temperature in the MLT region to the stratospheric sudden warmings (SSWs) during 2005 to 2021 in the Northern Hemisphere. The four meteor radars include the Svalbard (78.3°N, 16°E) and Tromsø (69.6°N, 19.2°E) meteor radars at high latitudes and the Mohe (53.5°N, 122.3°E) and Beijing (40.3°N, 116.2°E) meteor radars at middle latitudes. The superposed epoch analysis results indicate that: 1) the neutral density over Svalbard and Tromsø at high latitude increased at the beginning of SSWs and decreased after the zonal mean stratospheric temperature reached the maximum. However, the neutral density over Mohe at midlatitudes decreased in neutral density at the beginning of SSW and increase after the zonal mean stratospheric temperature reached the maximum. 2) The zonal wind at high latitudes show a westward enhancement at the beginning of SSWs and then shows an eastward enhancement after the stratospheric temperature reaches maximum. However, the zonal wind at midlatitudes shows an opposite variation to at high latitudes, with an eastward enhancement at the onset and changing to westward enhancements after the stratospheric temperature maximum. The meridional winds at high and midlatitudes show a southward enhancement after the onset of SSW and then show a northward enhancement after the stratospheric temperature maximum. 3) In general, the temperature in the MLT region decreased throughout SSWs. However, as the latitudes decrease, the temperature cooling appears to lag a few days to the higher latitudes, and the degree of cooling will decrease relatively.
How sport changed my life? Description of the perceived effects of the experiences of young Colombians throughout a sport for development and peace program
Authors: Gadais Tegwen , Varela Pulido Natalia , Soto Victoria , Vinazco Sandra , Garzon Mauricio
Published: May 2023

Introduction

This study contributes to the advancement of the field of Sport for Development and Peace (SDP) research in Latin America and the Caribbean (LAC). There are still few studies on SDP programs in this region and it is important to document and understand the impacts of these programs on participants.

Methods

The present study is the result of a collaborative research that aims to describe the experiences and perceptions of Colombian youth and program managers who participated in an SDP program that took them from a local community sports club to the Olympic Games. Seven semi-structured interviews were conducted with key actors (administrators, coaches, and athletes) who participated in a triple and transversal (local, district and national) Olympic walking training program.

Results

The results provided a better understanding of the program dynamics in the local, regional, and national level, as well as of the short- and long-term effects perceived by the actors of the process on their development, education, health, and career. Recommendations are made for SDP organizations in LAC.

Discussion

Future studies should continue to investigate the SDP initiative in LAC to understand how sport can help development and peace building in this region.
Porcine circovirus type 3: immunohistochemical detection in lesions of naturally affected piglets
Authors: Molossi Franciéli Adriane , Albuquerque de Almeida Bruno , Santana de Cecco Bianca , Pissetti Caroline , Ventura Lauren , Brandalise Luciano , Simão Gustavo , Vanucci Fabio , Negrao Watababe Tatiane Terumi , Vaz Jr. Itabajara da Silva , Driemeier David
Published: May 2023

This study aimed to evaluate the relationship between porcine circovirus type 3 (PCV3) viral load and histopathological findings in perinatal piglet tissues and to develop an immunohistochemical method for detecting the virus in lesions. The quantitative polymerase chain reaction (qPCR) cycle threshold (Ct) when amplifying PCV3 DNA and the area of perivascular inflammatory infiltrates in different organs [central nervous system (CNS), lung, heart, liver, spleen, and lymph nodes] were compared. To develop an immunohistochemistry technique, rabbit sera were produced against PCV3-capsid protein peptides selected using bioinformatic analyses. The assay was initially implemented using a tissue sample previously tested using qPCR and in situ hybridization to optimize the procedure and reagent dilutions. To evaluate immunohistochemistry performance, tissue samples from another 17 cases were analyzed using standardized parameters. The most common microscopic lesion was multisystemic periarteritis, with associated vasculitis, as the mesenteric vascular plexus is one of the most affected organs. Other tissues, such as the heart, lung, CNS, and skeletal muscle, were also affected. Comparison of the Ct values for different tissues showed no significant difference, except in lymphoid organs (spleen and lymph nodes), which had significantly higher viral loads than the CNS tissues. There was no correlation between Ct values and perivascular inflammatory infiltrates. PCV3 immunohistochemistry revealed granular immunolabeling, mainly in the cytoplasm of cells in the vascular mesenteric plexus, heart, lung, kidney, and spleen.
Pregnancy Complications and Impact on Kidney Allograft After Kidney Transplantation in IgA Nephropathy
Authors: Oki Rikako , Unagami Kohei , Kakogawa Jun , Beppu Hiroko , Banno Taro , Yagisawa Takafumi , Kanzawa Taichi , Hirai Toshihito , Omoto Kazuya , Kitajima Kumiko , Shirakawa Hiroki , Hoshino Junichi , Takagi Toshio , Ishida Hideki
Published: May 2023

Pregnancy in kidney transplantation (KT) recipients has been challenging because of the high risk of maternal, fetal, and renal complications. Although patients with immunoglobulin A nephropathy (IgAN)-chronic kidney disease (CKD) are at a high risk for hypertension in pregnancy (HIP), the maternal risk in KT recipients with IgAN as the etiology remains unclear. We retrospectively reviewed the medical records of pregnant KT recipients who delivered at our hospital. The incidence of maternal and fetal complications and the impact on kidney allografts between the group with IgAN as the primary kidney disease and the group with other primary diseases were compared. The analysis included 73 pregnancies in 64 KT recipients. The IgAN group had a higher incidence of HIP than the non-IgAN group (69% vs. 40%, p = 0.02). IgAN as primary kidney disease and interval from transplantation to conception were associated with HIP (OR 3.33 [1.11–9.92], p = 0.03, OR 0.83 [0.72–0.96], p < 0.01, respectively). The 20-year graft survival or prevention of CKD stage 5 in group with IgAN was lower than that in the group with other primary disease (p < 0.01). KT recipients should be informed of the risk of HIP and possibility of long-term worsening of postpartum renal function.
Giants with Feet of Clay? An Inquiry into User Payment Patterns for Subscription Video-on-Demand Services
Authors: Redondo Ignacio , Serrano Diana
Published: May 2023

Subscription video-on-demand platforms such as Netflix and HBO Max are being increasingly challenged by the widespread practice of sharing accounts with individuals outside the household. Platforms face a massive loss of revenue due to the opportunistic behavior of many users who enjoy content without paying anything or paying only a part of the required subscription fees. This study explores which factors influence platform users to pay all, part, or none of the subscription fees. Using a cross-sectional survey from Spain, various demographic, attitudinal, and behavioral factors were identified as predictors of the patterns of full, partial, and non-payment. The findings may help platform managers tailor some interventions to monetize a larger number of actual users by deterring the opportunistic payment patterns without discouraging the full payment pattern.
Detection of Mannheimia haemolytica-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle
Authors: Poonsuk Korakrit , Kordik Carita , Hille Matthew , Cheng Ting-Yu , Crosby William B. , Woolums Amelia R. , Clawson Michael L. , Chitko-McKown Carol , Brodersen Bruce , Loy John Dustin
Published: May 2023

Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera (n = 145) collected from feedlot cattle. The test showed good inter-assay and intra-assay repeatability. Diagnostic sensitivity and specificity were estimated at 91% and 87% for IgG at a cutoff of S/P ≥ 0.8. IgM diagnostic sensitivity and specificity were 91% and 81% at a cutoff of sample to positive (S/P) ratio ≥ 0.8. IgA diagnostic sensitivity was 89% whereas specificity was 78% at a cutoff of S/P ≥ 0.2. ELISA results of all isotypes were related to the diagnosis of respiratory disease and isolation of M. haemolytica (p-value < 0.05). These data suggest that M. haemolytica ELISAs can be adapted to the detection and quantification of antibody in serum specimens and support the use of these tests for the disease surveillance and disease prevention research in feedlot cattle.
Could Insect Products Provide a Safe and Sustainable Feed Alternative for the Poultry Industry? A Comprehensive Review
Authors: Khalifah Ayman , Abdalla Sara , Rageb Mai , Maruccio Lucianna , Ciani Francesca , El-Sabrout Karim
Published: May 2023

The planet is home to more than 2000 species of edible insects, some of which have been consumed as food for many years. Recently, edible insect products have been gradually increasing in several countries, such as Italy and Egypt, as novel feed resources for humans and animals due to their availability, potential economic benefits, and high nutritive value. The insect industry can provide a new solution for livestock nutrition and offer many additional advantages, but there are obstacles to overcome, such as some nutritional organizations that forbid its usage. Nevertheless, previous research indicates that different insect species could be used safely as nutraceuticals in poultry farming to improve broiler growth performance (>3%) and layer egg production (>5%). Among these species, there are various products and extracts that can be used in poultry nutrition in a sustainable manner. This review provides an outline of insect composition, nutrient values, application in poultry feed, safety, and guidelines, and finally, the future perspectives of insects as an alternative feed source in poultry diets.
Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins
Authors: Tomaszewska Ewa , Rudyk Halyna , Dobrowolski Piotr , Arciszewski Marcin B. , Donaldson Janine , Kras Katarzyna , Abramowicz Beata , Kuc Damian , Muszyński Siemowit
Published: May 2023

Animal feed is very frequently contaminated with different types of mold, the metabolites of which are toxic to living organisms. Mold-contaminated cereal is rich in heat-resistant and harmful metabolites such as fumonisins (FBs). The amount of FBs consumed as part of animal feed, including livestock feed, is unknown. Therefore, this study aimed to evaluate the effects of maternal oral FB intoxication on basal duodenum morphology and the immunolocalization of gut hormones responsible for food intake (leptin and ghrelin), as well as their receptors, in newborn rat offspring. Pregnant Wistar rats were randomly allocated to one of three groups: a control group or one of two FB-intoxicated groups (60 or 90 mg FB/kg b.w., respectively). Basal morphological duodenal parameters changed in a dose- and sex-dependent manner. The intensity of the ghrelin immunoreaction was unchanged in females, while in males it increased after FB exposure (60 mg/kg b.w.), with a simultaneous decrease in expression of the ghrelin receptor. Leptin and its receptor immunoreaction intensity was decreased in both sexes following FB exposure. The current study highlighted the potential involvement of intestinal ghrelin and leptin in the metabolic disturbances observed later in life in offspring that were prenatally exposed to fumonisins.
Aortic Aneurysm: Finding the Right Target
Authors: Kaschina Elena
Published: May 2023

This Special Issue of Biomedicines highlights many important scientific findings in aneurysm research [...]
How Sample Size Impacts Probabilistic Stimulation Maps in Deep Brain Stimulation
Authors: Nordin Teresa , Blomstedt Patric , Hemm Simone , Wårdell Karin
Published: May 2023

Probabilistic stimulation maps of deep brain stimulation (DBS) effect based on voxel-wise statistics (p-maps) have increased in literature over the last decade. These p-maps require correction for Type-1 errors due to multiple testing based on the same data. Some analyses do not reach overall significance, and this study aims to evaluate the impact of sample size on p-map computation. A dataset of 61 essential tremor patients treated with DBS was used for the investigation. Each patient contributed with four stimulation settings, one for each contact. From the dataset, 5 to 61 patients were randomly sampled with replacement for computation of p-maps and extraction of high- and low-improvement volumes. For each sample size, the process was iterated 20 times with new samples generating in total 1140 maps. The overall p-value corrected for multiple comparisons, significance volumes, and dice coefficients (DC) of the volumes within each sample size were evaluated. With less than 30 patients (120 simulations) in the sample, the variation in overall significance was larger and the median significance volumes increased with sample size. Above 120 simulations, the trends stabilize but present some variations in cluster location, with a highest median DC of 0.73 for n = 57. The variation in location was mainly related to the region between the high- and low-improvement clusters. In conclusion, p-maps created with small sample sizes should be evaluated with caution, and above 120 simulations in single-center studies are probably required for stable results.