bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Advances in Biliopancreatic Endoscopy
Authors: Anderloni Andrea , Khalaf Kareem
Published: April 2022

In this Special Issue of Medicina entitled “Recent Advances in Biliopancreatic Endoscopy” in the Section “Gastroenterology & Hepatology”, nine articles pave new insights into the advances in the world of biliopancreatic endoscopy [...]
Neurophysiological Effects of a Singing Bowl Massage
Authors: Walter Nike , Hinterberger Thilo
Published: April 2022

Background and Objectives: In recent years, singing bowl sound interventions have been progressively implemented in the fields of well-being, therapy and education; however, the effectiveness has only scarcely been investigated. Therefore, this study was aimed at determining neurophysiological effects of a singing bowl massage. Materials and Methods: In this prospective cohort study 64-channel EEG, ECG and respiration was recorded from 34 participants (mean age 36.03 ± 13.43 years, 24 females/10 males) before, during and after a professional singing bowl massage. Further, subjective changes in well-being were assessed. EEG data were analyzed by determining the effect sizes of distinct frequency bands. Significant differences were calculated by a two-tailed t-test corrected for multiple comparisons. Heart rate variability metrics, heart rate and respiration rate were estimated and compared. Results: Overall EEG power decreased during the sound condition compared to a task-free resting state (d = −0.30, p = 0.002). After the intervention, global EEG power was further reduced (d = −0.46, p < 0.001), revealing a decrease in the beta 2 (d = −0.15, p = 0.002) and the gamma frequency band (d = −0.21, p = 0.004). The mean heart rate was significantly lower after the intervention (75.5 ± 19.8 vs. 71.5 ± 17.9, p < 0.001) and the respiration rate higher (13.5 ± 5.3 vs. 15.2 ± 6.3, p = 0.018). 91.2% of the participants felt more integrated, 97.1% more balanced and 76.5% more vitalized. Conclusions: The neurophysiological effects of a singing bowl sound massage may be interpreted as a shift towards a more mindful, meditative state of consciousness. The intervention was perceived as beneficial for the wellbeing.
Whole-Body Vibration Effects on Flexibility in Artistic Gymnastics—A Systematic Review
Authors: Đorđević Dušan , Paunović Miloš , Čular Dražen , Vlahović Tomislav , Franić Miljenko , Sajković Dubravka , Petrović Tadija , Sporiš Goran
Published: April 2022

It is well documented that whole body-vibration training has effects on muscle strength and flexibility, blood circulation, decreases pain perception and strengthens bone and tendon. Although whole body-vibration has benefits in athletes’ flexibility, we are not sure what its actual effects are in artistic gymnastics (since they already have stunning flexibility). Hence, the aim of this study was to analyse the studies on whole-body vibration in artistic gymnastics and to present the effects on flexibility. The search and analysis were carried out in accordance with PRISMA guidelines. The databases search (PubMed, Scopus, Google Scholar, Cochrane Library, ProQuest, EBSCOhost and Science Direct) yielded 18,057 potential studies. By the given inclusion criteria (studies from 2005 to 2022; full-text published in English; the study included male and female gymnasts as samples, and that participants were tested for evaluation of flexibility by whole-body vibration method), a total of 9 full-text studies were included, with a total of 210 participants, both male and female. As far as the measured flexibility tests conducted, front split, sit and reach and bridge were evaluated, while obtained results were 9.1–39.1%, 2.79–6.7%, 6.43–7.45%, respectively. All studies have conducted same vibration frequency (30 Hz) with same amplitude of displacements (2 mm), except for the one study who did not show the information of implemented amplitude. After analysing the obtained results, it can be concluded that the usage of whole-body vibration platform shows flexibility improvements in artistic gymnasts, both male and female. In addition, a combination of whole-body vibration and traditional static stretching may enhance the flexibility in artistic gymnasts. However, these results should be taken with caution. Since this review did not reveal the optimal vibrational protocol, it is necessary to invest time during the implementation of various vibrational experimental protocols, so future research is required.
Polypropylene Hollow Fiber Membrane by Dissolution-Inducing Pore Methods
Authors: Qiu Zhongyong , He Chunju
Published: April 2022

Plasma leakage limits the development of polypropylene membranes as oxygenated membranes. Here, a new method named the dissolution-induced pore method was adapted to prepare polypropylene hollow fiber membranes: after polypropylene and polyvinyl chloride were melt-blended and extruded, the polyvinyl chloride was removed by N, N-dimethylacetamide to obtain a porous polypropylene membrane material. The variation of membranes has been explored in detail with respect to the influence of different parameters on the flux and mechanical properties of membranes and the feasibility of the polyvinyl chloride recovery. The resulting polypropylene hollow fiber membrane shows that plasma penetration was zero within 6 h of test, gas flux can reach 189,000 L/(m2·h·0.1 MPa), and its strength at break reaches 65 MPa and the elongation at break is 890%; polyvinyl chloride recovery achieves more than 99%. This research has developed a promising and low-cost extracorporeal membrane oxygenation material, which provides benefits for patients with less capacity for medical expenditure.
Liver Graft Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver Transplantation
Authors: Lin Shu-Hsien , Wang Chih-Chi , Huang Kuang-Tzu , Chen Kuang-Den , Hsu Li-Wen , Eng Hock-Liew , Chiu King-Wah
Published: April 2022

Background: Most cases of advanced liver diseases are associated with low serum 25-hydroxyvitamin D and vitamin D deficiency. This phenomenon may occur in living donor liver transplantation (LDLT). Aims: We conducted this study to explore the interplay between VDR and CYP2R1 in liver graft and compared our findings with the pathological interpretation of serum 25(OH)D concentration. Methods: In total, 60 patients received liver graft biopsy after LDLT and were separated (1:1) into two groups: graft rejection group and graft non-rejection group. We extracted both of the recipients’ and donors’ serum DNA to investigate the vitamin D receptor (VDR) rs2228530 and CYP2R1 rs10741657 single nucleotide polymorphisms (SNPs) using real-time polymerase chain reaction. We also extracted DNA from liver graft tissues to explore the genetic alleles of VDR rs2228530 and CYP2R1 rs10741657 after LDLT. Serum biochemistry profile and 25(OH)D concentrations were measured before and after LDLT. Results: There were no significant differences in serum VDR rs2228530 and CYP2R1 rs10741657 genetic alleles between recipients and donors. The percentage of genetic modification was 33.4% (10/30) for the rejection and non-rejection groups in VDR rs2228530, and 66.7% (20/30) for both groups in CYP2R1 rs10741657. Serum 25(OH)D concentrations were significantly lower after LDLT D30 than that before LDLT in the rejection (p = 0.0001) and non-rejection graft pathology (p = 0.0017) groups. Conclusions: The presence of low serum 25(OH)D concentrations after LDLT suggested that post-transplant low serum 25(OH)D concentrations may develop with the homogenous phenomenon of VDR rs2228530 and CYP2R1 rs10741657 genetic modifications in recipients regardless of graft pathology.
The Differential Metabolic Response of Oral Squamous Cell Carcinoma Cells and Normal Oral Epithelial Cells to Cisplatin Exposure
Authors: Chen Xun , Kuang Sufang , He Yi , Li Hongyu , Yi Chen , Li Yiming , Wang Chao , Chen Guanhui , Chen Shangwu , Yu Dongsheng
Published: April 2022

Metabolic reprogramming is one of the hallmarks of a tumor. It not only promotes the development and progression of tumor but also contributes to the resistance of tumor cells to chemotherapeutics. The difference in the metabolism between drug-resistant and sensitive tumor cells indicates that drug-resistant tumor cells have experienced metabolic adaptation. The metabolic response induced by chemotherapy is dynamic, but the early metabolic response of tumor cells to anticancer drugs and the effect of an initial response on the development of drug resistance have not been well studied. Early metabolic intervention may prevent or slow down the development of drug resistance. The differential metabolic responses of normal cells and tumor cells to drugs are unclear. The specific metabolites or metabolic pathways of tumor cells to chemotherapeutic drugs can be used as the target of metabolic intervention in tumor therapy. In this study, we used comparative metabolomics to analyze the differential metabolic responses of oral cancer cells and normal oral epithelial cells to short-term cisplatin exposure, and to identify the marker metabolites of early response in oral cancer cells. Oral cancer cells showed a dynamic metabolic response to cisplatin. Seven and five metabolites were identified as specific response markers to cisplatin exposure in oral cancer cell SCC-9 and normal oral epithelial cell HOEC, respectively. Glyoxylate and dicarboxylate metabolism and fructose, malate, serine, alanine, sorbose and glutamate were considered as specific enriched metabolic pathways and biomarkers of SCC-9 cells in response to cisplatin, respectively. The existence of differential metabolic responses lays a foundation for tumor chemotherapy combined with metabolic intervention.
Culicoides segnis and Culicoides pictipennis Biting Midges (Diptera, Ceratopogonidae), New Reported Vectors of Haemoproteus Parasites
Authors: Žiegytė Rita , Bernotienė Rasa , Palinauskas Vaidas
Published: April 2022

As bloodsuckers of birds, Culicoides biting midges (Diptera, Ceratopogonidae) play an important role in the transmission of avian haemosporidian (Haemoproteus) parasites, which are prevalent in many bird populations and cause disease, pathology, or even mortality in their hosts. Information about the role of the various Culicoides species in the transmission of Haemoproteus parasites remains insufficient. This presents an obstacle for the better understanding of the epizootiology of haemoproteosis. The aim of this study was to determine new Culicoides species involved in the transmission of Haemoproteus parasites in the wild. Biting midges were collected using UV traps on the Curonian Spit, Lithuania. Only parous Culicoides females were investigated: they were identified and were diagnosed for the presence of Haemoproteus parasites using both microscopy and PCR-based methods. We collected and dissected 420 parous Culicoides females. PCR-based screening showed that 28 parous Culicoides biting midges were infected with avian Haemoproteus parasites. Haemoproteid DNA was detected in Culicoides kibunensis, Culicoides pictipennis, Culicoides festivipennis, Culicoides segnis, Culicoides pallidicornis, and Culicoides obsoletus biting midges. The DNA of Haemoproteus palloris, genetic lineage hWW1, was found for the first time in C. pallidicornis. Haemoproteus sporozoites were detected in the salivary glands of two Culicoides segnis biting midges. According to the PCR results, one female contained Haemoproteus tartakovskyi (genetic lineage hHAWF1) DNA and another Haemoproteus majoris (genetic lineage hCCF5) DNA. The sporozoites of Haemoproteus parasites were also detected in the salivary glands of four C. pictipennis biting midges using microscopy, and this finding was confirmed by PCR as Haemoproteus parabelopolskyi DNA (genetic lineage hSYAT02) was detected in three out of the four biting midges. The obtained results supplement existing information about Culicoides biting midges as natural vectors of Haemoproteus spp. and add two new Culicoides species to the vector list, showing the low specificity of these parasites for the invertebrate hosts.
Molecular Epidemiology and Evolutionary Dynamics of Human Influenza Type-A Viruses in Africa: A Systematic Review
Authors: Nabakooza Grace , Galiwango Ronald , Frost Simon D. W. , Kateete David P. , Kitayimbwa John M.
Published: April 2022

Genomic characterization of circulating influenza type-A viruses (IAVs) directs the selection of appropriate vaccine formulations and early detection of potentially pandemic virus strains. However, longitudinal data on the genomic evolution and transmission of IAVs in Africa are scarce, limiting Africa’s benefits from potential influenza control strategies. We searched seven databases: African Journals Online, Embase, Global Health, Google Scholar, PubMed, Scopus, and Web of Science according to the PRISMA guidelines for studies that sequenced and/or genomically characterized Africa IAVs. Our review highlights the emergence and diversification of IAVs in Africa since 1993. Circulating strains continuously acquired new amino acid substitutions at the major antigenic and potential N-linked glycosylation sites in their hemagglutinin proteins, which dramatically affected vaccine protectiveness. Africa IAVs phylogenetically mixed with global strains forming strong temporal and geographical evolution structures. Phylogeographic analyses confirmed that viral migration into Africa from abroad, especially South Asia, Europe, and North America, and extensive local viral mixing sustained the genomic diversity, antigenic drift, and persistence of IAVs in Africa. However, the role of reassortment and zoonosis remains unknown. Interestingly, we observed substitutions and clades and persistent viral lineages unique to Africa. Therefore, Africa’s contribution to the global influenza ecology may be understated. Our results were geographically biased, with data from 63% (34/54) of African countries. Thus, there is a need to expand influenza surveillance across Africa and prioritize routine whole-genome sequencing and genomic analysis to detect new strains early for effective viral control.
Microbiota Transplantation in an Antibiotic-Induced Bacterial Depletion Mouse Model: Reproducible Establishment, Analysis, and Application
Authors: Shang Lijun , Tu Jiayu , Dai Ziqi , Zeng Xiangfang , Qiao Shiyan
Published: April 2022

The fecal bacteria transplantation (FMT) technique is indispensable when exploring the pathogenesis and potential treatments for microbiota-related diseases. For FMT clinical treatments, there are already systematic guidelines for donor selection, fecal bacterial separation, FMT frequency, and infusion methods. However, only a few studies have demonstrated the use of standardized FMT procedures for animal models used in theoretical research, creating difficulties for many new researchers in this field. In the present paper, we provide a brief overview of FMT and discuss its contribution to the current understanding of disease mechanisms that relate to microbiota. This protocol can be used to generate a commonly used FMT mouse model and provides a literature reference of customizable steps.
Identification of a New Endo-β-1,4-xylanase Prospected from the Microbiota of the Termite Heterotermes tenuis
Authors: Alcobaça Olinda S. A. , Campanini Emeline B. , Ciancaglini Iara , Rocha Sâmara V. , Malavazi Iran , Freire Caio C. M. , Nunes Francis M. F. , Fuentes Andrea S. C. , Cunha Anderson F.
Published: April 2022

Xylanases are hemicellulases that break down xylan to soluble pentoses. They are used for industrial purposes, such as paper whitening, beverage clarification, and biofuel production. The second-generation bioethanol production is hindered by the enzymatic hydrolysis step of the lignocellulosic biomass, due to the complex arrangement established among its constituents. Xylanases can potentially increase the production yield by improving the action of the cellulolytic enzyme complex. We prospected endo-β-1,4-xylanases from meta-transcriptomes of the termite Heterotermes tenuis. In silico structural characterization and functional analysis of an endo-β-1,4-xylanase from a symbiotic protist of H. tenuis indicate two active sites and a substrate-binding groove needed for the catalytic activity. No N-glycosylation sites were found. This endo-β-1,4-xylanase was recombinantly expressed in Pichia pastoris and Escherichia coli cells, presenting a molecular mass of approximately 20 kDa. Enzymatic activity assay using recombinant endo-β-1,4-xylanase was also performed on 1% xylan agar stained with Congo red at 30 °C and 40 °C. The enzyme expressed in both systems was able to hydrolyze the substrate xylan, becoming a promising candidate for further analysis aiming to determine its potential for application in industrial xylan degradation processes.
Changes in Invasive Neisseria meningitidis and Haemophilus influenzae Infections in France during the COVID-19 Pandemic
Authors: Deghmane Ala-Eddine , Taha Muhamed-Kheir
Published: April 2022

BackgroundSince the appearance of COVID-19 in January 2020, invasive bacterial infections have decreased significantly worldwide. However, alterations in age and sex distributions, clinical forms, phenotypes, and genotypes of isolates have not been analyzed. Our goal is to present and discuss these data considering the current COVID-19 pandemic situation. Methods: The data of the national reference center for meningococci and Haemophilus influenzae in France were mined to examine the above aspects of invasive bacterial infection before (2018–2019) and after (2020–2021) the COVID-19 pandemic. Detailed epidemiological, clinical, and microbiological data were collected, and whole genome sequencing was carried out on meningococcal isolates (n = 1466). Results: In addition to the overall decline in the number of cases, various changes in age, sex, and phenotypes of isolates were also noted. As for N. meningitidis, more cases were observed in adults, as well as more invasive pneumopathies. Furthermore, fewer hyperinvasive meningococcal genotypes have circulated since COVID-19 emerged. The situation has been different for H. influenzae, as the number of invasive cases among adults decreased due to a reduction in non-typeable isolates. In contrast, cases due to serotypeable isolates, particularly serotypes a and b, increased in children <5 years-old. Conclusions: It is possible that measures implemented to stop COVID-19 may have reduced the circulation of N. meningitidis and H. influenzae isolates, but to a variable extent. This may be due to differences in circulation between these two species according to age groups. Vaccination schedules against these two species may have also influenced the evolution of these invasive bacterial infections since the emergence of the COVID-19 pandemic.
Evaluation of the In Vitro Antiparasitic Effect of the Essential Oil of Cymbopogon winterianus and Its Chemical Composition Analysis
Authors: Pereira Pedro Silvino , Oliveira Carlos Vinicius Barros , Maia Ana Josicleide , Vega-Gomez Maria Celeste , Rolón Miriam , Coronel Cathia , Duarte Antônia Eliene , Coutinho Henrique Douglas Melo , Siyadatpanah Abolghasem , Norouzi Roghayeh , Sadati Seyed Jafar Adnani , Wilairatana Polrat , Silva Teresinha Gonçalves
Published: April 2022

Cymbopogon winterianus, known as “citronella grass”, is an important aromatic and medicinal tropical herbaceous plant. The essential oil of C. winterianus (EOCw) is popularly used to play an important role in improving human health due to its potential as a bioactive component. The present study aimed to identify the components of the essential oil of C. winterianus and verify its leishmanicidal and trypanocidal potential, as well as the cytotoxicity in mammalian cells, in vitro. The EOCw had geraniol (42.13%), citronellal (17.31%), and citronellol (16.91%) as major constituents. The essential oil only exhibited significant cytotoxicity in mammalian fibroblasts at concentrations greater than 250 μg/mL, while regarding antipromastigote and antiepimastigote activities, they presented values considered clinically relevant, since both had LC50 < 62.5 μg/mL. It can be concluded that this is a pioneer study on the potential of the essential oil of C. winterianus and its use against the parasites T. cruzi and L. brasiliensis, and its importance is also based on this fact. Additionally, according to the results, C. winterianus was effective in presenting values of clinical relevance and low toxicity and, therefore, an indicator of popular use.
New Cysteine-Containing PEG-Glycerolipid Increases the Bloodstream Circulation Time of Upconverting Nanoparticles
Authors: Nikolaeva Maria E. , Nechaev Andrey V. , Shmendel Elena V. , Akasov Roman A. , Maslov Mikhail A. , Mironov Andrey F.
Published: April 2022

Upconverting nanoparticles have unique spectral and photophysical properties that make them suitable for development of theranostics for imaging and treating large and deep-seated tumors. Nanoparticles based on NaYF4 crystals doped with lanthanides Yb3+ and Er3+ were obtained by the high-temperature decomposition of trifluoroacetates in oleic acid and 1-octadecene. Such particles have pronounced hydrophobic properties. Therefore, to obtain stable dispersions in aqueous media for the study of their properties in vivo and in vitro, the polyethylene glycol (PEG)-glycerolipids of various structures were obtained. To increase the circulation time of PEG-lipid coated nanoparticles in the bloodstream, long-chain substituents are needed to be attached to the glycerol backbone using ether bonds. To prevent nanoparticle aggregation, an L-cysteine-derived negatively charged carboxy group should be included in the lipid molecule.
An Efficient Chromatin Immunoprecipitation Protocol for the Analysis of Histone Modification Distributions in the Brown Alga Ectocarpus
Authors: Bourdareau Simon , Godfroy Olivier , Gueno Josselin , Scornet Delphine , Coelho Susana M. , Tirichine Leila , Cock J. Mark
Published: April 2022

The brown algae are an important but understudied group of multicellular marine organisms. A number of genetic and genomic tools have been developed for the model brown alga Ectocarpus; this includes, most recently, chromatin immunoprecipitation methodology, which allows genome-wide detection and analysis of histone post-translational modifications. Post-translational modifications of histone molecules have been shown to play an important role in gene regulation in organisms from other major eukaryotic lineages, and this methodology will therefore be a very useful tool to investigate genome function in the brown algae. This article provides a detailed, step-by-step description of the Ectocarpus ChIP protocol, which effectively addresses the difficult problem of efficiently extracting chromatin from cells protected by a highly resistant cell wall. The protocol described here will be an essential tool for the future application of chromatin analysis methodologies in brown algal research.
Reactivity of Vanadium Nanoparticles with Oxygen and Tungsten
Authors: Morales Francisco Miguel , Escanciano Marta , Yeste María Pilar , Santos Antonio Jesús
Published: April 2022

A mechanistic study was carried out on the optimal methods of fabrication of products containing higher loads of thermochromic VO2(M1) fabricated by thermal treatments of V nanoparticles in air, that, once achieved, are more stable than other commercial products upon natural aging or reiterated reheating. At the best temperatures for single runs, 55% of VO2 can be attained by the reactions of a limited number of the species initially formed in a process, that, if not stopped, can degrade the product by solid state reactions of oxidations and reductions without O2 consumption. This fact supports the use of two-step treatments at lower temperatures and faster cooling rates that reach 65% of VO2; such reactions should, ideally, take place in the 550–625 °C temperature range. The impregnation of V with a tungstate salt is an ideal and simple doping platform that can decrease the energy of activation of the 2-cycle process, allowing higher yields and enthalpies of transformation (71% of VO2, 26 J/g) than undoped counterparts or trademarks. A good balance is reached for 1% at. of W, with a reduction in Tc of 20 °C not significantly resenting the enthalpy of the reversible metal-to-insulator transition. For higher W amounts, the appearance of tetragonal VO2, and W alloyed V3O7 and V2O5, decrease the fractions of increasingly and effectively doped M1-VO2 achieved till 2% of W, a concentration for which Tc attains the stimulating values of 35 °C on heating and 25 °C on cooling.
A Narrative Review about Autism Spectrum Disorders and Exclusion of Gluten and Casein from the Diet
Authors: González-Domenech Pablo José , Diaz-Atienza Francisco , Gutiérrez-Rojas Luis , Fernández-Soto María Luisa , González-Domenech Carmen María
Published: April 2022

Objective: Autism spectrum disorders (ASDs) appear in the early stages of neurodevelopment, and they remain constant throughout life. Currently, due to limitations in ASDs treatment, alternative approaches, such as nutritional interventions, have frequently been implemented. The aim of this narrative review is to gather the most relevant and updated studies about dietary interventions related to ASDs etiopathogenesis. Results: Our literature search focused on the gluten- and casein-free (GFCF) diet. The literature found shows the inexistence of enough scientific evidence to support a general recommendation of dietary intervention in children with ASD. Protocols and procedures for assessing risk and safety are also needed. Future lines: Prospective and controlled research studies with larger sample sizes and longer follow-up times are scarce and needed. In addition, studies considering an assessment of intestinal permeability, bacterial population, enzymatic, and inflammatory gastrointestinal activity are interesting to identify possible responders. Besides brain imaging techniques, genetic tests can also contribute as markers to evaluate the comorbidity of gastrointestinal symptoms.
Association of Cesarean Delivery with Trajectories of Growth and Body Composition in Preschool Children
Authors: Liao Zijun , Wang Jing , Chen Fangfang , Chen Yiren , Zhang Ting , Liu Gongshu , Xie Xianghui , Tai Jun
Published: April 2022

This study aimed to examine the association of cesarean delivery with trajectories of growth and body composition in preschool children. This ambidirectional cohort study was conducted between 2017 and 2020 in China. Information on the delivery mode, weight, and length/height of the children measured at routine healthcare visits was obtained from maternal and child health records. For three years while in kindergarten, children’s body mass index (BMI), fat mass index (FMI), fat-free mass index (FFMI), and percentage of body fat (FM%) were repeatedly measured. A BMI z score (zBMI) was calculated and standardized to WHO measures, and overweight and obesity were defined using the WHO reference. After adjustment for maternal age, maternal education, annual family income, prepregnancy BMI, gestational weight gain, gravidity, parity, gestational age, child sex, birthweight, breastfeeding duration, and the parent-reported dietary intake of the children, children born via cesarean delivery (n = 1992) versus those born vaginally (n = 1578) had higher zBMI growth rates beyond 36 months (β: 0.003; 95% CI: 0.001, 0.005 SD units/month) and elevated levels of FMI (β: 0.097; 95% CI: 0.026, 0.168 kg/m2), FM% (β: 0.402; 95% CI: 0.058, 0.745%) and zBMI (β: 0.073; 95% CI: 0.012, 0.133 units), but not FFMI (β: 0.022; 95% CI: −0.022, 0.066 kg/m2). The adjusted OR of overweight and obesity was 1.21 (95% CI: 1.04, 1.40). Cesarean delivery likely elevated zBMI growth rates and increased the risk of overweight and obesity in preschool children, with the elevation of fat mass but not fat-free mass.
Complementary Feeding and Malnutrition among Infants and Young Children Aged 6–23 Months in Rural Areas of China
Authors: Feng Jing , Gong Zhaolong , Wang Yongjun , Huo Junsheng , Zhuo Qin
Published: April 2022

This study investigated the nutrition and complementary feeding (CF) of infants and young children (IYC) aged 6–23 months in rural areas of China in 2018 and explored the relationship between CF and nutritional status. We measured the length and weight, calculated the z-scores, and detected micronutrients in the hair. The status of CF was obtained from the respondents by a 24-h dietary recall. IYC were classified into clusters using a two-step cluster analysis. The CF and nutritional status of each cluster were analyzed and compared. The prevalence of stunting, wasting, and overweight in the IYC in rural Chinese areas was 7.1%, 3.0%, and 3.7%, respectively. The median levels of Ca, Fe, and Zn in hair were 550.10 µg/g, 62.94 µg/g, and 132.86 µg/g, respectively. The prevalence of meeting the requirements of minimum dietary diversity (MDD), minimum meal frequency (MMF), and minimum acceptable diet (MAD) was 68.9%, 77.9%, and 46.4%, respectively. IYC with a higher prevalence of MDD, MMF, and MAD were more inclined to maintain a healthy status. The prevalence of undernutrition and overweight of 6- to 23-month-old IYC in rural areas of China was low. However, lack of trace elements was evident, and MAD prevalence remained low.
Chitosan Nanoparticles in Atherosclerosis—Development to Preclinical Testing
Authors: Sriamornsak Pornsak , Dass Crispin R.
Published: April 2022

Chitosan is a natural biopolymer that is present in an abundant supply in sources such as crustacean shells, mushrooms, and insect exoskeletons. It can be used to make a variety of types of drug formulations and is generally safe to use in vivo; plus, it has inherent cholesterol-reducing properties. While an abundance of papers has tested this biopolymer in nanoparticles in cancer and diabetes research, there is a lag of usage, and hence the paucity of information, in the area of cardiovascular research, specifically in atherosclerosis, the topic of this review. This review highlights some of the deficiencies in this niche area of research, examines the range of chitosan nanoparticles that have been researched to date, and proposes several ways forward to advance this field. Nanoparticles used for both diagnostic and therapeutic purposes are reviewed, with a discussion on how these nanoparticles could be better researched in future and what lays ahead as the field potentially moves towards clinical trials in future.
Significant Electromechanical Characteristic Enhancement of Coaxial Electrospinning Core–Shell Fibers
Authors: Nguyen Duc-Nam , Moon Wonkyu
Published: April 2022

Electrospinning is a low-cost and straightforward method for producing various types of polymers in micro/nanofiber form. Among the various types of polymers, electrospun piezoelectric polymers have many potential applications. In this study, a new type of functional microfiber composed of poly(γ-benzyl-α,L-glutamate) (PBLG) and poly(vinylidene fluoride) (PVDF) with significantly enhanced electromechanical properties has been reported. Recently reported electrospun PBLG fibers exhibit polarity along the axial direction, while electrospun PVDF fibers have the highest net dipole moment in the transverse direction. Hence, a combination of PBLG and PVDF as a core–shell structure has been investigated in the present work. On polarization under a high voltage, enhancement in the net dipole moment in each material and the intramolecular conformation was observed. The piezoelectric coefficient of the electrospun PBLG/PVDF core–shell fibers was measured to be up to 68 pC N−1 (d33), and the voltage generation under longitudinal extension was 400 mVpp (peak-to-peak) at a frequency of 60 Hz, which is better than that of the electrospun homopolymer fibers. Such new types of functional materials can be used in various applications, such as sensors, actuators, smart materials, implantable biosensors, biomedical engineering devices, and energy harvesting devices.
Punching Shear Strength of FRP-Reinforced Concrete Slabs without Shear Reinforcements: A Reliability Assessment
Authors: Alkhatib Soliman , Deifalla Ahmed
Published: April 2022

The recent failure of buildings because of punching shear has alerted researchers to assess the reliability of the punching shear design models. However, most of the current research studies focus on model uncertainty compared to experimentally measured strength, while very limited studies consider the variability of the basic variables included in the model and the experimental measurements. This paper discusses the reliability of FRP-reinforced concrete slabs’ existing punching shear models. First, more than 180 specimens were gathered. Second, available design codes and simplified models were selected and used in the calculation. Third, several reliability methods were conducted; therefore, three methods were implemented, including the mean-value first-order second moment (MVFOSM) method, the first-order second moment (FOSM) method, and the second-order reliability method (SORM). A comparison between the three methods showed that the reliability index calculated using the FOSM is quite similar to that using SORM. However, FOSM is simpler than SORM. Finally, the reliability and sensitivity of the existing strength models were assessed. At the same design point, the reliability index varied significantly. For example, the most reliable was the JSCE, with a reliability index value of 4.78, while the Elgendy-a was the least reliable, with a reliability index of 1.03. The model accuracy is the most significant parameter compared to other parameters, where the sensitivity factor varied between 67% and 80%. On the other hand, the column dimension and flexure reinforcement are the least significant parameters compared to other parameters where the sensitivity factor was 0.4% and 0.3%, respectively.
Open Tubular Column Immobilized with Covalent Organic Frameworks for Rapid Separation of Small Molecular Compounds by Capillary Electrochromatography
Authors: Gao Lidi , Dong Qing , Zhao Xuan , Hu Xingfang , Chu Hongtao , Lv Renjiang , Qin Shili
Published: April 2022

Preparation of a novel TpBD (synthesized from phloroglucinol and benzidine) covalent organic framework (COF) immobilized open-tubular (OT) capillary is described by in situ growth strategy. The stationary phase in the column was characterized by Fourier transform infrared (FTIR) spectra, X-ray diffraction (XRD), thermogravimetric analysis (TGA), nitrogen adsorption–desorption isotherms, scanning electron microscope (SEM), transmission electron microscopy (TEM) and energy-dispersive X-ray spectrum (EDS). Several families of compounds with different properties (alkylbenzenes, parabens, sulfonamides and benzoic acids) were selected to evaluate the performance of the TpBD COF immobilized capillary. The results showed that the stationary phase was uniform with about 6.0 μm thickness under the optimal preparation conditions, and the relative standard deviations (RSDs) were no more than 3.13% of alkylbenzenes on the TpBD COF immobilized capillary for 11 consecutive runs, which exhibited its excellent reproducibility and stability. A rapid baseline separation of each family of the analytes (neutral parabens, amphoteric sulfonamides and acidic benzoic acids) was obtained in less than 6 min with a resolution (Rs) of 2.79~9.30, which sufficiently verified the rapid separation, high resolution and wide application range of the TpBD COF immobilized capillary, and further revealed this strategy of fabricating COF to capillary column to show great promise in capillary electrochromatography.
Water Body Super-Resolution Mapping Based on Multiple Endmember Spectral Mixture Analysis and Multiscale Spatio-Temporal Dependence
Authors: Yang Xiaohong , Chu Qiannian , Wang Lizhe , Yu Menghui
Published: April 2022

Water body mapping is an effective way to monitor dynamic changes in surface water, which is of great significance for water resource management. Super-resolution mapping is a valid method to generate high-resolution dynamic water body maps from low-spatial-resolution images. However, the accuracy of existing super-resolution mapping methods is not high due to the low accuracy of fraction images and the insufficiency of spatial pattern information. To solve this problem, this paper proposes a spectral similarity scale-based multiple-endmember spectral mixture analysis (SSS-based MESMA) and a multiscale spatio-temporal dependence method based on super-resolution mapping (MESMA_MST_SRM) for water bodies. SSS-based MESMA allows different coarse pixels to have different endmember combinations, which can effectively improve the accuracy of spectral unmixing and then improve the accuracy of fraction images. Multiscale spatio-temporal dependence adopts both pixel-based and subpixel-based spatial dependence. In this study, eight different types of water body mappings derived from the Landsat 8 Operational Land Imager (OLI) and Google Earth images were employed to test the performance of the MESMA_MST_SRM method. The results of the eight experiments showed that compared with the other four tested methods, the overall accuracy (OA) value, as well as the overall distribution and detailed information of the water map generated by the MESMA_MST_SRM method, were the best, indicating the great potential and efficiency of the proposed method in water body mapping.
Vertical Ground Displacements and Its Impact on Erosion along the Karachi Coastline, Pakistan
Authors: Kanwal Shamsa , Ding Xiaoli , Wu Songbo , Sajjad Muhammad
Published: April 2022

This study employed remote sensing (optical and synthetic aperture radar) and data analysis techniques to quantify vertical ground displacements and assess their contribution to coastline erosion. To provide evidence from Pakistan, we selected the coast of Karachi—a mega-city located along the dynamic coastline of the Indus River Delta—which has been experiencing severe coastal erosion during the last few decades. Observations from the C-band Envisat/ASAR and Sentinel-1A sensors over the 2004–2010 and 2014–2016 periods, respectively, enabled us to study vertical ground displacements in the study area, providing a long-term assessment during 2004–2016. Results suggest that some areas along the Karachi coastline are subsiding at comparable rates to or even much higher than the relative sea-level rise (SLR, ~1.9 mm/yr), which may amplify the rates of relative SLR in coming years, along with accelerating coastal erosion. Various parts of the study area along the coast are unstable and undergoing displacement. Landsat images from 1989 to 2018 (10-year temporal resolution) were further used to examine the state of coastline erosion using three statistical approaches (i.e., End Point Rate (EPR), Linear Regression Rate (LRR), and Least Median of Squares (LMS)). While the erosion underlaid the majority of the eastern sections of the study area, the ground displacements were spatially heterogeneous across the study area and along the coastline. Erosion rates of ~2.4 m/yr spatially corresponded with ground displacement rates of up to ~−1.4 cm/yr, but not all the coastline segments with high annual mean erosion rates were associated with local mean subsidence. The causes of ground displacements and coastline erosion were analyzed, and results were interpreted by integrating spatial ancillary information. Results indicate that rapid urbanization, construction on reclaimed land, coastline erosion favoring seawater intrusion, failed drainage/sewerage networks, and soil liquefaction are contributing to the site-specific variations in the land displacement in Karachi.
Road Extraction Convolutional Neural Network with Embedded Attention Mechanism for Remote Sensing Imagery
Authors: Shao Shiwei , Xiao Lixia , Lin Liupeng , Ren Chang , Tian Jing
Published: April 2022

Roads are closely related to people’s lives, and road network extraction has become one of the most important remote sensing tasks. This study aimed to propose a road extraction network with an embedded attention mechanism to solve the problem of automatic extraction of road networks from a large number of remote sensing images. Channel attention mechanism and spatial attention mechanism were introduced to enhance the use of spectral information and spatial information based on the U-Net framework. Moreover, residual densely connected blocks were introduced to enhance feature reuse and information flow transfer, and a residual dilated convolution module was introduced to extract road network information at different scales. The experimental results showed that the method proposed in this study outperformed the compared algorithms in overall accuracy. This method had fewer false detections, and the extracted roads were closer to ground truth. Ablation experiments showed that the proposed modules could effectively improve road extraction accuracy.
Finite Mixture Models in the Evaluation of Positional Accuracy of Geospatial Data
Authors: Rodríguez-Avi José , Ariza-López Francisco Javier
Published: April 2022

Digital elevation models (DEMs) are highly relevant geospatial products, and their positional accuracy has demonstrated influence on elevation derivatives (e.g., slope, aspect, curvature, etc.) and GIS results (e.g., drainage network and watershed delineation, etc.). The accuracy assessment of the DEMs is usually based on analyzing the altimetric component by means of positional accuracy assessment methods that are based on the use of a normal distribution for error modeling but, unfortunately, the observed distribution of the altimetric errors is not always normal. This paper proposes the application of a finite mixture model (FMM) to model altimetric errors. The way to adjust the FMM is provided. Moreover, the behavior under sampling is analyzed when applying different positional accuracy assessment standards such as National Map Accuracy Standards (NMAS), Engineering Map Accuracy Standard (EMAS) and National Standard for Spatial Data Accuracy (NSSDA) under the consideration of the FMM and the traditional approach-based one-single normal distribution model (1NDM). For the NMAS, the FMM performs statistically much better than the 1NDM when considering all the tolerance values and sample sizes. For the EMAS, the type I error level is around 3.5 times higher in the case of the 1NDM than in the case of the FMM. In the case of the NSSDA, as it has been applied in this research (simple comparison of values, not hypothesis testing), there is no great difference in behavior. The conclusions are clear; the FMM offers results that are always more consistent with the real distribution of errors, and with the supposed statistical behavior of the positional accuracy assessment standard when based on hypothesis testing.
A New Orbiting Deployable System for Small Satellite Observations for Ecology and Earth Observation
Authors: Martellato Elena , Piccirillo Alice Maria , Ferraioli Giampaolo , Rotundi Alessandra , Della Corte Vincenzo , Palumbo Pasquale , Alcaras Emanuele , Appolloni Luca , Aulicino Giuseppe , Bertini Ivano , Capozzi Vincenzo , Catucci Elena , Dionnet Zelia , Di Palma Pasquale , Esposito Flavio , Ferrentino Emanuele , Innac Anna , Inno Laura , Pennino Silvia , Saviano Simona , Tirimberio Giuseppina , Campopiano Stefania , Chianese Elena , Franzese Pier Paolo , Fusco Giannetta , Gaglione Salvatore , Iadicicco Agostino , Nunziata Ferdinando , Parente Claudio , Piscopo Vincenzo , Riccio Angelo , Russo Giovanni Fulvio , Zambianchi Enrico
Published: April 2022

In this paper, we present several study cases focused on marine, oceanographic, and atmospheric environments, which would greatly benefit from the use of a deployable system for small satellite observations. As opposed to the large standard ones, small satellites have become an effective and affordable alternative access to space, owing to their lower costs, innovative design and technology, and higher revisiting times, when launched in a constellation configuration. One of the biggest challenges is created by the small satellite instrumentation working in the visible (VIS), infrared (IR), and microwave (MW) spectral ranges, for which the resolution of the acquired data depends on the physical dimension of the telescope and the antenna collecting the signal. In this respect, a deployable payload, fitting the limited size and mass imposed by the small satellite architecture, once unfolded in space, can reach performances similar to those of larger satellites. In this study, we show how ecology and Earth Observations can benefit from data acquired by small satellites, and how they can be further improved thanks to deployable payloads. We focus on DORA—Deployable Optics for Remote sensing Applications—in the VIS to TIR spectral range, and on a planned application in the MW spectral range, and we carry out a radiometric analysis to verify its performances for Earth Observation studies.
Characterization of Extra-Cellular Vesicle Dielectrophoresis and Estimation of Its Electric Properties
Authors: Chen Hao , Yamakawa Tsubasa , Inaba Masafumi , Nakano Michihiko , Suehiro Junya
Published: April 2022

Dielectrophoresis (DEP) refers to a type of electrical motion of dielectric particles. Because DEP is caused by particle polarization, it has been utilized to characterize particles. This study investigated the DEP of three types of exosomes, namely bovine milk, human breast milk, and human breast cancer exosomes. Exosomes are kinds of extracellular vesicles. The crossover frequencies of the exosomes were determined by direct observation of their DEPs. Consequently, bovine and human milk exosomes showed similar DEP properties, whereas the cancer exosomes were significantly different from the others. The membrane capacitance and conductivity of the exosomes were estimated using determined values. A significant difference was observed between bovine and human milk exosomes on their membrane capacitance. It was revealed that the membrane capacitances of human breast milk and human breast cancer exosomes were almost identical to those of their host cells and the conductivity of the exosomes were much lower than that of the host cell. Based on these results, DEP separation of the human breast milk and cancer exosomes was demonstrated. These results imply that DEP can be utilized to separate and identify cancer exosomes rapidly. Additionally, our method can be utilized to estimate the electric property of other types of extracellular vesicles.
Opening the Black Box: Bootstrapping Sensitivity Measures in Neural Networks for Interpretable Machine Learning
Authors: La Rocca Michele , Perna Cira
Published: April 2022

Artificial neural networks are powerful tools for data analysis, particularly in the context of highly nonlinear regression models. However, their utility is critically limited due to the lack of interpretation of the model given its black-box nature. To partially address the problem, the paper focuses on the important problem of feature selection. It proposes and discusses a statistical test procedure for selecting a set of input variables that are relevant to the model while taking into account the multiple testing nature of the problem. The approach is within the general framework of sensitivity analysis and uses the conditional expectation of functions of the partial derivatives of the output with respect to the inputs as a sensitivity measure. The proposed procedure extensively uses the bootstrap to approximate the test statistic distribution under the null while controlling the familywise error rate to correct for data snooping arising from multiple testing. In particular, a pair bootstrap scheme was implemented in order to obtain consistent results when using misspecified statistical models, a typical characteristic of neural networks. Numerical examples and a Monte Carlo simulation were carried out to verify the ability of the proposed test procedure to correctly identify the set of relevant features.