bluuebunny/crossref_metadata_2025 huggingface dataset:
Hot Deformation Behavior and Workability of In-Situ TiB2/7050Al Composites Fabricated by Powder Metallurgy
Authors: Zhu Haofei , Liu Jun , Wu Yi , Zhang Qing , Shi Qiwei , Chen Zhe , Wang Lei , Zhang Fengguo , Wang Haowei
Published: November 2020

Isothermal compression tests of in-situ TiB2/7050Al composites fabricated by powder metallurgy were performed at 300–460 °C with the strain rate varying from 0.001 s−1 to 1 s−1. The Arrhenius constitutive equation and hot processing map of composites were established, presenting excellent hot workability with low activation energies and broad processing windows. Dramatic discontinuous/continuous dynamic recrystallization (DDRX/CDRX) and grain boundary sliding (GBS) take place in composites during deformation, depending on the Zener-Hollomon parameter (Z) values. It is found that initially uniform TiB2 particles and fine grain structures are beneficial to the DDRX, which is the major softening mechanism in composites at high Z values. With the Z value decreasing, dynamic recovery and CDRX around particles are enhanced, preventing the occurrence of DDRX. In addition, fine grain structures in composites are stable at elevated temperature thanks to the pinning of dense nanoparticles, which triggers the occurrence of GBS and ensures good workability at low Z values.
Estimating the Academic Performance of Secondary Education Mathematics Students: A Gain Lift Predictive Model
Authors: Trujillo-Torres Juan-Manuel , Hossein-Mohand Hassan , Gómez-García Melchor , Hossein-Mohand Hossein , Hinojo-Lucena Francisco-Javier
Published: November 2020

Several socioeconomic, environmental, ethnic, family, and educational factors influence an individual’s academic performance and can determine their school performance in mathematics. Mathematical competence is one of the skills that allow students to build visions of the future from performance in the present. However, the perception that students have of mathematics, in addition to the teacher–student relationship, the classroom, gender, teaching–learning, and motivation are crucial factors for achieving an optimal academic performance and preventing school failure. The aim of the present study was: (1) to examine which variables of the dimensions “Learning Mathematics” and “School Environment” significantly contribute to the marks in the second quarter and quantify their relative importance; (2) to determine the optimal algorithm model for predicting the maximum gain in students’ marks in the second quarter and quantifying it; and (3) to analyze the maximum gain in terms of gender. A total of 2018 high school students in Melilla were included in this cross-sectional study. Mathematical learning and the school environment were assessed using a validated 14-item questionnaire. Gain lift was employed to quantify the improvement in students’ performance. The role of the classroom and teacher–student relationship had a greater influence on mathematics scores than affinity indicators, teaching, study time, teaching resources used, study aids, and motivation.
The (Patho)Biology of SRC Kinase in Platelets and Megakaryocytes
Authors: De Kock Lore , Freson Kathleen
Published: November 2020

Proto-oncogene tyrosine-protein kinase SRC (SRC), as other members of the SRC family kinases (SFK), plays an important role in regulating signal transduction by different cell surface receptors after changes in the cellular environment. Here, we reviewed the role of SRC in platelets and megakaryocytes (MK). In platelets, inactive closed SRC is coupled to the β subunit of integrin αIIbβ3 while upon fibrinogen binding during platelet activation, αIIbβ3-mediated outside-in signaling is initiated by activation of SRC. Active open SRC now further stimulates many downstream effectors via tyrosine phosphorylation of enzymes, adaptors, and especially cytoskeletal components. Functional platelet studies using SRC knockout mice or broad spectrum SFK inhibitors pointed out that SRC mediates their spreading on fibrinogen. On the other hand, an activating pathological SRC missense variant E527K in humans that causes bleeding inhibits collagen-induced platelet activation while stimulating platelet spreading. The role of SRC in megakaryopoiesis is much less studied. SRC knockout mice have a normal platelet count though studies with SFK inhibitors point out that SRC could interfere with MK polyploidization and proplatelet formation but these inhibitors are not specific. Patients with the SRC E527K variant have thrombocytopenia due to hyperactive SRC that inhibits proplatelet formation after increased spreading of MK on fibrinogen and enhanced formation of podosomes. Studies in humans have contributed significantly to our understanding of SRC signaling in platelets and MK.
Cut-off Values of the Respiratory Muscle Power and Peak Cough Flow in Post-Stroke Dysphagia
Authors: Han Yeon Jae , Lee Jungjae , Sohn Dong Gyun , Park Geun-Young , Kim Youngkook , Park Hae-Yeon , Jung Sang-A , Im Sun
Published: November 2020

Background and objectives: This study aimed to determine the cut-off values of the following three respiratory pressure meters; the voluntary peak cough flow (PCF), maximal expiratory pressure (MEP) and maximal inspiratory pressure (MIP); associated with post-stroke dysphagia and assess which of these parameters show good diagnostic properties associated with post-stroke dysphagia. Materials and Methods: Retrospective analysis of a prospectively maintained database. Records of patients with first-ever diagnosed dysphagia attributable to cerebrovascular disease, who had performed spirometry measurements for the PCF, MIP and MEP. Results: From a total of 237 stroke patients, 163 patients were diagnosed with dysphagia. Those with dysphagia had significantly lower PCF values than those without dysphagia (116.3 ± 75.3 vs. 219.4 ± 91.8 L/min, p < 0.001). In addition, the former group also had lower MIP (30.5 ± 24.7 vs. 41.6 ± 25.7 cmH2O, p = 0.0002) and MEP (41.0 ± 27.9 vs. 62.8 ± 32.3 cmH2O, p < 0.001) values than the latter group. The receiver operating characteristic curve analysis showed that the PCF cut-off value of 151 L/min (area under the receiver operating characteristic curve [AUC] 0.81; sensitivity 72%; specificity 78.8%) was associated with post-stroke dysphagia. The optimum MEP and MIP cut-off were 38 cmH2O (AUC 0.70, sensitivity 58%; specificity 77.7%) and 20 cmH2O (AUC 0.65, sensitivity 49%; specificity 84%). PCF showed the highest AUC results. Results from the univariate analysis indicated that PCF values of ≤151 L/min increased risk of dysphagia by 9.51-fold (4.96–18.23). Multivariable analysis showed that after controlling of other clinical factor, the PCFs at this cut-off value still showed increased risk of by 4.19 (2.02–83.69) but this was not observed with the MIPs or MEPs. Conclusions: Our study has provided cut-off values that are associated with increased risk of dysphagia. Among the three parameters, PCF showed increased association with post-stroke dysphagia.
Tempforming as an Advanced Processing Method for Carbon Steels
Authors: Dolzhenko Anastasiya , Kaibyshev Rustam , Belyakov Andrey
Published: November 2020

The microstructural mechanisms providing delamination toughness in high-strength low-alloyed steels are briefly reviewed. Thermo-mechanical processing methods improving both the strength and impact toughness are described, with a close relation to the microstructures and textures developed. The effect of processing conditions on the microstructure evolution in steels with different carbon content is discussed. Particular attention is paid to tempforming treatment, which has been recently introduced as a promising processing method for high-strength low-alloyed steel semi-products with beneficial combination of strength and impact toughness. Tempforming consists of large strain warm rolling following tempering. In contrast to ausforming, the steels subjected to tempforming may exhibit an unusual increase in the impact toughness with a decrease in test temperature below room temperature. This phenomenon is attributed to the notch blunting owing to easy splitting (delamination) crosswise to the principle crack propagation. The relationships between the crack propagation mode, the delamination fracture, and the load-displacement curve are presented and discussed. Further perspectives of tempforming applications and promising research directions are outlined.
Wearing Behavior of the α-Al9FeMnSi Intermetallic Compound Formed by Reactive Sintering onto AISI 304L Stainless Steel
Authors: Salazar Ibarra Martín , Flores Valdés Alfredo , Escobedo Bocardo José Concepción , Flores Saldívar Alfredo Alan
Published: November 2020

In this study, using mixtures of pure Al, Si, Mn, and Fe powders, the α-Al9FeMnSi intermetallic compound was formed onto AISI 304L stainless steel samples by reactive sintering. The processing parameters were temperature (800, 850, and 900 °C) and applied pressure (15 and 20 MPa), using a constant holding time of 7200 s. In this paper, the influence of pressure and temperature on the microstructure, microhardness, and wear resistance of the formed layers was studied. Using X-ray diffraction (XRD), scanning electron microscopy (SEM), microhardness testing, and wearing measurements (pin on disc tests), the cross-section and top side of the coatings were observed and analyzed. We were able to determine the phase composition of cladded layers and interfaces as well as their morphology. The results indicated that several layers were formed during reactive sintering, i.e., an Al-diffusion layer on the top of the substrate, an interface, and the α-Al9FeMnSi coating itself. The microhardness values of the different layers formed were determined, ranging from 400 to 500 HV for the intermetallic coating, to 120 HV for the substrate. In this way, it was found that the formed intermetallic coating is suitable to increase the corrosion resistance of stainless steel. Additionally, all the coating showed high adherence to the substrate, exhibiting high microhardness and wear resistance. Pin on disc wearing tests showed the wearing mechanisms are predominantly delamination and ablation of the cladded layers and substrate.
Application of Differential Network Enrichment Analysis for Deciphering Metabolic Alterations
Authors: Iyer Gayatri R. , Wigginton Janis , Duren William , LaBarre Jennifer L. , Brandenburg Marci , Burant Charles , Michailidis George , Karnovsky Alla
Published: November 2020

Modern analytical methods allow for the simultaneous detection of hundreds of metabolites, generating increasingly large and complex data sets. The analysis of metabolomics data is a multi-step process that involves data processing and normalization, followed by statistical analysis. One of the biggest challenges in metabolomics is linking alterations in metabolite levels to specific biological processes that are disrupted, contributing to the development of disease or reflecting the disease state. A common approach to accomplishing this goal involves pathway mapping and enrichment analysis, which assesses the relative importance of predefined metabolic pathways or other biological categories. However, traditional knowledge-based enrichment analysis has limitations when it comes to the analysis of metabolomics and lipidomics data. We present a Java-based, user-friendly bioinformatics tool named Filigree that provides a primarily data-driven alternative to the existing knowledge-based enrichment analysis methods. Filigree is based on our previously published differential network enrichment analysis (DNEA) methodology. To demonstrate the utility of the tool, we applied it to previously published studies analyzing the metabolome in the context of metabolic disorders (type 1 and 2 diabetes) and the maternal and infant lipidome during pregnancy.
Five-Dimensional Optical Data Storage Based on Ellipse Orientation and Fluorescence Intensity in a Silver-Sensitized Commercial Glass
Authors: Park Chang-Hyun , Petit Yannick , Canioni Lionel , Park Seung-Han
Published: November 2020

Five-dimensional (5D) recording and decoding is demonstrated by using femtosecond direct laser writing in a silver-containing commercial glass. In particular, laser intensities and ellipse orientations generated by anamorphic focusing are employed to produce 5D data storage unit (3D for XYZ, 1D for the orientation of the elliptically-shaped data storage unit and 1D for its fluorescence intensity). In the recording process, two different images of a 4-bit bitmap format were simultaneously embedded in the medium by multiplexing the elliptical orientation of the laser focus and its intensity so as to access oriented elliptical patterns with independent fluorescence intensity. In the decoding process, two merged original images were successfully reconstructed by comparing each data storage unit with a fabricated calibration matrix of 16 × 16 levels for elliptic orientations and fluorescence intensities. We believe this technique can be applied to semi-permanent high-density data storage device.
A Method to Determine the Minimum Chip Thickness during Longitudinal Turning
Authors: Skrzyniarz Michal
Published: November 2020

Micromachining, which is used for various industrial purposes, requires the depth of cut and feed to be expressed in micrometers. Appropriate stock allowance and cutting conditions need to be selected to ensure that excess material is removed in the form of chips. To calculate the allowance, it is essential to take into account the tool nose radius, as this cutting parameter affects the minimum chip thickness. Theoretical and numerical studies on the topic predominate over experimental ones. This article describes a method and a test setup for determining the minimum chip thickness during turning. The workpiece was ground before turning to prevent radial runout and easily identify the transition zone. Contact and non-contact profilometers were used to measure surface profiles. The main aim of this study was to determine the tool–workpiece interaction stages and the cutting conditions under which material was removed as chips. Additionally, it was necessary to analyze how the feed, cutting speed, and edge radius influenced the minimum chip thickness. This parameter was found to be dependent on the depth of cut and feed. Elastic and plastic deformation and ploughing were observed when the feed rate was lower than the cutting edge radius.
Sensitivity Analysis of Single-Drive, 3-axis MEMS Gyroscope Using COMSOL Multiphysics
Authors: Din Hussamud , Iqbal Faisal , Lee Byeungleul
Published: November 2020

In this paper, a COMSOL Multiphysics-based methodology is presented for evaluation of the microelectromechanical systems (MEMS) gyroscope. The established finite element analysis (FEA) model was successfully validated through a comparison with analytical and Matlab/Simulink analysis results. A simplified single-drive, 3-axis MEMS gyroscope was analyzed using a mode split approach, having a drive resonant frequency of 24,918 Hz, with the x-sense, y-sense, and z-sense being 25,625, 25,886, and 25,806 Hz, respectively. Drive-mode analysis was carried out and a maximum drive-displacement of 4.0 μm was computed for a 0.378 μN harmonic drive force. Mechanical sensitivity was computed at 2000 degrees per second (dps) input angular rate while the scale factor for roll, pitch, and yaw was computed to be 0.014, 0.011, and 0.013 nm/dps, respectively.
Combined Magnetic, Transient Electromagnetic, and Magnetotelluric Methods to Detect a BIF-Type Concealed Iron Ore Body: A Case Study in Gongchangling Iron Ore Concentration Area, Southern Liaoning Province, China
Authors: Fu Jianfei , Jia Sanshi , Wang Ende
Published: November 2020

The detection and evaluation of concealed mineral resources deep in metallic mines and in the surrounding areas remain technically difficult. In particular, due to the complex topographic and geomorphic conditions on the surface, the detection environments in these areas limit the choices of detection equipment and data collection devices. In this study, based on metallogenic theory and the metallogenic geological characteristics of banded iron formation (BIF)-type iron ores, equipment for surface geophysical surveys (i.e., the high-precision ground magnetic survey method, the transient electromagnetic method, and the magnetotelluric method) and data collection devices capable of taking single-point continuous measurements were employed to detect the concealed iron ore bodies in the transition zone CID-1 between the Hejia and Dumu iron deposits in the Gongchangling iron ore concentration area in the Anshan-Benxi area (Liaoyang, China), a representative area of BIF-type iron ores. The results showed that an optimal combination of these geophysical survey methods accurately determined the anomalous planar spatial locations and anomalous profile morphologies of the concealed iron ore bodies. On this basis, we determined their locations, burial depths, and scales. Two anomalous zones induced by concealed iron ore bodies, YC-1 and YC-2, were discovered in zone CID-1. Two concealed iron-bearing zones, one shallow (0–150 m) and one deep (300–450 m), were found in YC-1. A 100 m scale drilling test showed that the cumulative thickness of the shallow iron-bearing zone was over 23.6 m.
Chemometric Modeling of Trace Element Data for Origin Determination of Demantoid Garnets
Authors: Bindereif Stefan , Rüll Felix , Schwarzinger Stephan , Schwarzinger Clemens
Published: November 2020

The determination of country of origin poses a common problem in the appraisal of gemstones and is in many cases still based on the observation of inclusions and growth features of a gem, whereas chemical analysis is only done by major labs. We have used Laser Ablation Inductively Coupled Plasma Mass Spectrometry to analyze the trace element profiles of demantoid garnets from six different countries and identified a set of six elements, which are magnesium, aluminum, titanium, vanadium, chromium, and manganese, that are necessary to assign the mining regions with a good certainty. By using the logarithms of the trace element concentrations and subjecting them to chemometric modeling, we were able to separate the demantoids originating from Russia, Pakistan, Namibia, Iran, and Madagascar very well, leaving only Italy with some uncertainty. Results are presented for an “all origins” model as well as pair-wise comparison of two locations at a time, which lead to even better results.
The Dynamics of the Neuropeptide Y Receptor Type 1 Investigated by Solid-State NMR and Molecular Dynamics Simulation
Authors: Vogel Alexander , Bosse Mathias , Gauglitz Marcel , Wistuba Sarah , Schmidt Peter , Kaiser Anette , Gurevich Vsevolod V. , Beck-Sickinger Annette G. , Hildebrand Peter W. , Huster Daniel
Published: November 2020

We report data on the structural dynamics of the neuropeptide Y (NPY) G-protein-coupled receptor (GPCR) type 1 (Y1R), a typical representative of class A peptide ligand GPCRs, using a combination of solid-state NMR and molecular dynamics (MD) simulation. First, the equilibrium dynamics of Y1R were studied using 15N-NMR and quantitative determination of 1H-13C order parameters through the measurement of dipolar couplings in separated-local-field NMR experiments. Order parameters reporting the amplitudes of the molecular motions of the C-H bond vectors of Y1R in DMPC membranes are 0.57 for the Cα sites and lower in the side chains (0.37 for the CH2 and 0.18 for the CH3 groups). Different NMR excitation schemes identify relatively rigid and also dynamic segments of the molecule. In monounsaturated membranes composed of longer lipid chains, Y1R is more rigid, attributed to a higher hydrophobic thickness of the lipid membrane. The presence of an antagonist or NPY has little influence on the amplitude of motions, whereas the addition of agonist and arrestin led to a pronounced rigidization. To investigate Y1R dynamics with site resolution, we conducted extensive all-atom MD simulations of the apo and antagonist-bound state. In each state, three replicas with a length of 20 μs (with one exception, where the trajectory length was 10 μs) were conducted. In these simulations, order parameters of each residue were determined and showed high values in the transmembrane helices, whereas the loops and termini exhibit much lower order. The extracellular helix segments undergo larger amplitude motions than their intracellular counterparts, whereas the opposite is observed for the loops, Helix 8, and termini. Only minor differences in order were observed between the apo and antagonist-bound state, whereas the time scale of the motions is shorter for the apo state. Although these relatively fast motions occurring with correlation times of ns up to a few µs have no direct relevance for receptor activation, it is believed that they represent the prerequisite for larger conformational transitions in proteins.
Preferred Endocytosis of Amyloid Precursor Protein from Cholesterol-Enriched Lipid Raft Microdomains
Authors: Cho Yoon Young , Kwon Oh-Hoon , Chung Sungkwon
Published: November 2020

Amyloid precursor protein (APP) at the plasma membrane is internalized via endocytosis and delivered to endo/lysosomes, where neurotoxic amyloid-β (Aβ) is produced via β-, γ-secretases. Hence, endocytosis plays a key role in the processing of APP and subsequent Aβ generation. β-, γ-secretases as well as APP are localized in cholesterol-enriched lipid raft microdomains. However, it is still unclear whether lipid rafts are the site where APP undergoes endocytosis and whether cholesterol levels affect this process. In this study, we found that localization of APP in lipid rafts was increased by elevated cholesterol level. We also showed that increasing or decreasing cholesterol levels increased or decreased APP endocytosis, respectively. When we labeled cell surface APP, APP localized in lipid rafts preferentially underwent endocytosis compared to nonraft-localized APP. In addition, APP endocytosis from lipid rafts was regulated by cholesterol levels. Our results demonstrate for the first time that cholesterol levels regulate the localization of APP in lipid rafts affecting raft-dependent APP endocytosis. Thus, regulating the microdomain localization of APP could offer a new therapeutic strategy for Alzheimer’s disease.
Medicinal Potential of Isoflavonoids: Polyphenols That May Cure Diabetes
Authors: Ahmed Qamar Uddin , Ali Abdul Hasib Mohd , Mukhtar Sayeed , Alsharif Meshari A. , Parveen Humaira , Sabere Awis Sukarni Mohmad , Nawi Mohamed Sufian Mohd. , Khatib Alfi , Siddiqui Mohammad Jamshed , Umar Abdulrashid , Alhassan Alhassan Muhammad
Published: November 2020

In recent years, there is emerging evidence that isoflavonoids, either dietary or obtained from traditional medicinal plants, could play an important role as a supplementary drug in the management of type 2 diabetes mellitus (T2DM) due to their reported pronounced biological effects in relation to multiple metabolic factors associated with diabetes. Hence, in this regard, we have comprehensively reviewed the potential biological effects of isoflavonoids, particularly biochanin A, genistein, daidzein, glycitein, and formononetin on metabolic disorders and long-term complications induced by T2DM in order to understand whether they can be future candidates as a safe antidiabetic agent. Based on in-depth in vitro and in vivo studies evaluations, isoflavonoids have been found to activate gene expression through the stimulation of peroxisome proliferator-activated receptors (PPARs) (α, γ), modulate carbohydrate metabolism, regulate hyperglycemia, induce dyslipidemia, lessen insulin resistance, and modify adipocyte differentiation and tissue metabolism. Moreover, these natural compounds have also been found to attenuate oxidative stress through the oxidative signaling process and inflammatory mechanism. Hence, isoflavonoids have been envisioned to be able to prevent and slow down the progression of long-term diabetes complications including cardiovascular disease, nephropathy, neuropathy, and retinopathy. Further thoroughgoing investigations in human clinical studies are strongly recommended to obtain the optimum and specific dose and regimen required for supplementation with isoflavonoids and derivatives in diabetic patients.
An Effective Hybrid Heterogeneous Catalyst to Desulfurize Diesel: Peroxotungstate@Metal–Organic Framework
Authors: Gao Yan , Mirante Fátima , de Castro Baltazar , Zhao Jianshe , Cunha-Silva Luís , Balula Salete S.
Published: November 2020

A peroxotungstate composite comprising the chromium terephthalate metal–organic framework MIL-101(Cr) and the Venturello peroxotungstate [PO4{WO(O2)2}4]3− (PW4) has been prepared by the impregnation method. The PW4@MIL-101(Cr) composite presents high catalytic efficiency for oxidative desulfurization of a multicomponent model diesel containing the most refractory sulfur compounds present in real fuels (2000 ppm of total S). The catalytic performance of this heterogeneous catalyst is similar to the corresponding homogeneous PW4 active center. Desulfurization efficiency of 99.7% was achieved after only 40 min at 70 °C using H2O2 as an oxidant and an ionic liquid as an extraction solvent ([BMIM]PF6, 2:1 model diesel/[BMIM]PF6). High recycling and reusing capacity was also found for PW4@MIL-101(Cr), maintaining its activity for consecutive oxidative desulfurization cycles. A comparison of the catalytic performance of this peroxotungstate composite with others previously reported tungstate@MIL-101(Cr) catalysts indicates that the presence of active oxygen atoms from the peroxo groups promotes a higher oxidative catalytic efficiency in a shorter reaction time.
Macrooxazoles A–D, New 2,5-Disubstituted Oxazole-4-Carboxylic Acid Derivatives from the Plant Pathogenic Fungus Phoma macrostoma
Authors: Matio Kemkuignou Blondelle , Treiber Laura , Zeng Haoxuan , Schrey Hedda , Schobert Rainer , Stadler Marc
Published: November 2020

In our ongoing search for new bioactive fungal metabolites, four previously undescribed oxazole carboxylic acid derivatives (1–4) for which we proposed the trivial names macrooxazoles A–D together with two known tetramic acids (5–6) were isolated from the plant pathogenic fungus Phoma macrostoma. Their structures were elucidated based on high-resolution mass spectrometry (HR-MS) and nuclear magnetic resonance (NMR) spectroscopy. The hitherto unclear structure of macrocidin Z (6) was also confirmed by its first total synthesis. The isolated compounds were evaluated for their antimicrobial activities against a panel of bacteria and fungi. Cytotoxic and anti-biofilm activities of the isolates are also reported herein. The new compound 3 exhibited weak-to-moderate antimicrobial activity as well as the known macrocidins 5 and 6. Only the mixture of compounds 2 and 4 (ratio 1:2) showed weak cytotoxic activity against the tested cancer cell lines with an IC50 of 23 µg/mL. Moreover, the new compounds 2 and 3, as well as the known compounds 5 and 6, interfered with the biofilm formation of Staphylococcus aureus, inhibiting 65%, 75%, 79%, and 76% of biofilm at 250 µg/mL, respectively. Compounds 5 and 6 also exhibited moderate activity against S. aureus preformed biofilm with the highest inhibition percentage of 75% and 73% at 250 µg/mL, respectively.
Characterization of Beet Root Extract (Beta vulgaris) Encapsulated with Maltodextrin and Inulin
Authors: Flores-Mancha Martha A. , Ruíz-Gutiérrez Martha G. , Sánchez-Vega Rogelio , Santellano-Estrada Eduardo , Chávez-Martínez América
Published: November 2020

Betalains are powerful antioxidants contained in beets. These are divided into betacyanins (red-violet) and betaxanthins (yellow-orange), and they can be used as natural colorants in the food industry. The effects of freeze-drying pure beet juice (B) and the encapsulation of beet juice with a dextrose equivalent (DE) 10 maltodextrin (M) and agave inulin (I) as carrier agents were evaluated. The powders showed significant differences (p < 0.05) in all the variables analyzed: water absorption index (WAI), water solubility index (WSI), glass transition temperature (Tg), total betalains (TB), betacyanins (BC), betaxanthins (BX), total polyphenols (TP), antioxidant activity (AA, via 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH)) and total protein concentration (TPC). The highest values of antioxidant activity were found in the non-encapsulated beet powder, followed by the powder encapsulated with maltodextrin and, to a lesser extent, the powder encapsulated with inulin. The glass transition temperature was 61.63 °C for M and 27.59 °C for I. However, for B it was less than 18.34 °C, which makes handling difficult. Encapsulation of beet extract with maltodextrin and inulin by lyophilization turned out to be an efficient method to increase solubility and diminish hygroscopicity.
Novel Small-Molecule Scaffolds as Candidates against the SARS Coronavirus 2 Main Protease: A Fragment-Guided in Silico Approach
Authors: Augustin Teresa L. , Hajbabaie Roxanna , Harper Matthew T. , Rahman Taufiq
Published: November 2020

The ongoing pandemic caused by the novel coronavirus has been the greatest global health crisis since the Spanish flu pandemic of 1918. Thus far, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in over 1 million deaths, and there is no cure or vaccine to date. The recently solved crystal structure of the SARS-CoV-2 main protease has been a major focus for drug-discovery efforts. Here, we present a fragment-guided approach using ZINCPharmer, where 17 active fragments known to bind to the catalytic centre of the SARS-CoV-2 main protease (SARS-CoV-2 Mpro) were used as pharmacophore queries to search the ZINC databases of natural compounds and natural derivatives. This search yielded 134 hits that were then subjected to multiple rounds of in silico analyses, including blind and focused docking against the 3D structure of the main protease. We scrutinised the poses, scores, and protein–ligand interactions of 15 hits and selected 7. The scaffolds of the seven hits were structurally distinct from known inhibitor scaffolds, thus indicating scaffold novelty. Our work presents several novel scaffolds as potential candidates for experimental validation against SARS-CoV-2 Mpro.
Physical and Sensory Properties of Japanese Quince Chips Obtained by Osmotic Dehydration in Fruit Juice Concentrates and Hybrid Drying
Authors: Kowalska Hanna , Marzec Agata , Domian Ewa , Masiarz Ewelina , Ciurzyńska Agnieszka , Galus Sabina , Małkiewicz Aleksandra , Lenart Andrzej , Kowalska Jolanta
Published: November 2020

Japanese quince has high health value, but due to its taste and texture, it is difficult to eat raw. The use of innovative drying methods to produce dried snack foods from these fruits may be of interest to producers and consumers. The physicochemical and sensory properties of 3 mm slices of Japanese quince fruit (with skin, without seeds) obtained by osmotic pre-treatment in chokeberry and apple juice concentrates, and with the use of convection (convective drying, C-D), freeze-drying (F-D), and convection-microwave-vacuum drying (hybrid) are assessed. The methods of drying osmo-dehydrated slices do not affect the dry matter content. In most dried quince, the water activity is 0.40 or lower. Pre-osmotic dehydration and drying have a significant impact on the mechanical and acoustic properties of quince chips. Sensory attractive chips emit loud acoustic emission (AE) during the breaking test. Chips that are osmo-dehydrated in a mixture of chokeberry juice concentrate and sucrose and dried by a hybrid method are attractive. They have a dark red color given by chokeberry concentrate and a slight sweet (with a slight sour-bitter) taste. The sensory evaluation was useful for determining the quality of the chips in terms of their texture (crispness) tested by mechanical methods. Their sensory ratings (overall desirability as weight of color, taste, crispness, and flavor) are high and similar (from 3.8 to 4.1). The use of innovative drying methods with pre-osmotic treatment allows obtaining dried material with properties comparable to those obtained by the F-D method, but in a much shorter time, i.e., with lower energy and using a simple method.
Design for Breathtaking Experiences: An Exploration of Design Strategies to Evoke Awe in Human–Product Interactions
Authors: Ke Jialin , Yoon JungKyoon
Published: November 2020

From looking up at a skyscraper to the Grand Canyon’s vastness, you may have experienced awe in one way or another. Awe is experienced when one encounters something greater or more powerful than themselves and is associated with prosocial behavior through a diminishment of self-importance. In design research, most studies on awe have been conducted in lab conditions by using technologies such as virtual reality because of its efficiency to simulate typical awe-stimulating conditions (e.g., nature scenes). While useful in inducing awe and assessing its effects on users, they give little guidance about how design can deliberately evoke awe. Most attempts focus on the response of awe instead of its eliciting conditions. With an aim to support designers to facilitate awe, this paper explores design strategies to evoke awe. Based on appraisal theory, the cause of awe was formulated, and its relevance to designing for awe was investigated. The conditions that underlie awe in design were explored through a survey in which participants reported 150 awe experiences, resulting in six design strategies. The paper describes these strategies and discusses how they can be used in a design process, giving attention to addressing the experiential value of awe.
Magnetic Ionic Liquid Nanocatalyst to Improve Mechanical and Thermal Properties of Epoxy Nanocomposites
Authors: Atta Ayman M. , Al-Lohedan Hamad A. , Tawfeek Ahmed M. , Sabeela Nourah I.
Published: November 2020

New magnetic imidazolium ionic liquid (IIL) was synthesized to improve the curing, mechanical, and thermal characteristics of the epoxy/polyamine system. In this respect, 2-(4-minophenyl)-1.3-bis(triethoxysilyl)-1H-imidazol-3-ium acetate as IIL was synthesized and characterized by different spectroscopy tools. The IIL was used as capping to prepare Fe3O4 nanoparticles (NPs) as new Fe3O4-IIL NPs. The thermal stability, morphology, crystal lattice structures, and magnetic properties were evaluated to confirm the formation of uniform, thermal, stable, and superparamagnetic Fe3O4-IIL NPs. The prepared Fe3O4-IIL NPs were mixed with an epoxy/polyamine system to improve the curing, thermal, and mechanical properties of epoxy through chemical reactions. The dynamic mechanical analyzer and differential scanning calorimeter were used to investigate the flexibility and storage modulus of the cured epoxy/polyamine system in the absence and presence of Fe3O4-IIL NPs. The atomic force microscope and scanning electron microscope were used to evaluate the dispersion and embedding of Fe3O4-IIL NPs into epoxy matrix. The thermal, mechanical, and surface morphologies data confirmed that the incorporation of Fe3O4-IIL NPs using 3 wt. % during the curing of an epoxy/polyamine system produces superior epoxy films without cracks, holes, and NPs agglomeration.
Validation and Reliability of the Alzheimer’s Disease-Commonwealth Scientific and Industrial Research Organisation Food Frequency Questionnaire
Authors: Gardener Samantha L. , Lyons-Wall Philippa , Martins Ralph N. , Rainey-Smith Stephanie R.
Published: November 2020

Accuracy in measuring intake of dietary constituents is an important issue in studies reporting the associations between diet and chronic diseases. We modified a Commonwealth Scientific and Industrial Research Organisation (CSIRO) food frequency questionnaire (FFQ) to include foods of interest in the field of Alzheimer’s disease (AD) research. The aim of the current study was to determine the reliability and validity of the AD-CSIROFFQ in 148 cognitively normal older adults. The AD-CSIROFFQ was completed before and after completion of a four-day weighed food record. Of the 508 food and beverage items reported, 309 had sufficient consumption levels for analysis of reliability. Of the 309 items, over 78% were significantly correlated between the two questionnaire administrations (Spearman’s rank correlations). We used two additional methods to assess absolute nutrient intake agreement between the AD-CSIROFFQ and the weighed food records (Pearson’s correlation coefficients and Bland–Altman plots) and quintile rankings to measure group level agreement. The adequate correlations observed between questionnaire responses suggest that the AD-CSIROFFQ is reliable. All nutrient intakes were acceptable for ranking of individuals on a group level, whilst the agreement levels with respect to the weighed food records for 11 of the 46 nutrients show validity in terms of their individual level absolute intake. The AD-CSIROFFQ makes an important contribution to the tools available for assessing usual dietary intake in groups of older adults with respect to AD research.
Probiotic Bacillus Spores Together with Amino Acids and Immunoglobulins Exert Protective Effects on a Rat Model of Ulcerative Colitis
Authors: Catinean Adrian , Neag Maria Adriana , Krishnan Kiran , Muntean Dana Maria , Bocsan Corina Ioana , Pop Raluca Maria , Mitre Andrei Otto , Melincovici Carmen Stanca , Buzoianu Anca Dana
Published: November 2020

In inflammatory bowel disease (IBD), experimental models have proven to be important tools for evaluating potential therapeutic agents and for investigating the mechanisms of pathogenesis. Oxidative stress and the immune response have been associated with acetic acid (AA)-induced ulcerative colitis (UC). Our study aimed to evaluate, for the first time, the ability of a spore-based probiotic and an amino acid and immunoglobulin supplement in reducing tissue damage and inflammatory responses in an experimental animal model of UC. Forty-two Wistar rats were divided into six groups, receiving 1% carboxymethylcellulose, 4% AA, MegaSporeBiotic™ (MSB; 1 × 109 colony forming units/day) and MegaMucosa™ (MM; 70 mg/100 g/day). Pretreatment with MSB or MM alone and in combination significantly lowered inflammation and reduced damage to the colonic mucosa. Pretreatment with these agents resulted in levels of proinflammatory cytokines, vascular tight junction proteins, and measures of oxidative stress similar to those reported for methylprednisolone, one of the first-line therapies for moderate to severe activity of UC. The protection was further confirmed by histologic analysis of the colon tissue. In conclusion, pretreatment with probiotic spore-forming Bacillus strains and a supplement of amino acids in combination with immunoglobulins exhibited anti-inflammatory and antioxidant effects in an AA-induced rat model of UC.
Tofacitinib Loaded Squalenyl Nanoparticles for Targeted Follicular Delivery in Inflammatory Skin Diseases
Authors: Christmann Rebekka , Ho Duy-Khiet , Wilzopolski Jenny , Lee Sangeun , Koch Marcus , Loretz Brigitta , Vogt Thomas , Bäumer Wolfgang , Schaefer Ulrich F. , Lehr Claus-Michael
Published: November 2020

Tofacitinib (TFB), a Janus kinase inhibitor, has shown excellent success off-label in treating various dermatological diseases, especially alopecia areata (AA). However, TFB’s safe and targeted delivery into hair follicles (HFs) is highly desirable due to its systemic adverse effects. Nanoparticles (NPs) can enhance targeted follicular drug delivery and minimize interfollicular permeation and thereby reduce systemic drug exposure. In this study, we report a facile method to assemble the stable and uniform 240 nm TFB loaded squalenyl derivative (SqD) nanoparticles (TFB SqD NPs) in aqueous solution, which allowed an excellent loading capacity (LC) of 20%. The SqD NPs showed an enhanced TFB delivery into HFs compared to the aqueous formulations of plain drug in an ex vivo pig ear model. Furthermore, the therapeutic efficacy of the TFB SqD NPs was studied in a mouse model of allergic dermatitis by ear swelling reduction and compared to TFB dissolved in a non-aqueous mixture of acetone and DMSO (7:1 v/v). Whereas such formulation would not be acceptable for use in the clinic, the TFB SqD NPs dispersed in water illustrated a better reduction in inflammatory effects than plain TFB’s aqueous formulation, implying both encouraging good in vivo efficacy and safety. These findings support the potential of TFB SqD NPs for developing a long-term topical therapy of AA.
Solid Lipid Nanoparticles as Formulative Strategy to Increase Oral Permeation of a Molecule Active in Multidrug-Resistant Tuberculosis Management
Authors: Obinu Antonella , Porcu Elena Piera , Piras Sandra , Ibba Roberta , Carta Antonio , Molicotti Paola , Migheli Rossana , Dalpiaz Alessandro , Ferraro Luca , Rassu Giovanna , Gavini Elisabetta , Giunchedi Paolo
Published: November 2020

The role of mycobacterial efflux pumps in drug-resistant tuberculosis has been widely reported. Recently, a new compound, named SS13, has been synthesized, and its activity as a potential efflux inhibitor has been demonstrated. In this work, the chemical–physical properties of the SS13 were investigated; furthermore, a formulative study aimed to develop a formulation suitable for oral administration was performed. SS13 shows nonintrinsic antitubercular activity, but it increases the antitubercular activity of all the tested drugs on several strains. SS13 is insoluble in different simulated gastrointestinal media; thus, its oral absorption could be limited. Solid lipid nanoparticles (SLNs) were, therefore, developed by using two different lipids, Witepsol and/or Gelucire. Nanoparticles, having a particle size (range of 200–450 nm with regards to the formulation composition) suitable for intestinal absorption, are able to load SS13 and to improve its permeation through the intestinal mucosa compared to the pure compound. The cytotoxicity is influenced by the concentration of nanoparticles administered. These promising results support the potential application of these nanocarriers for increasing the oral permeation of SS13 in multidrug-resistant tuberculosis management.
Repurposing of Fluvastatin as an Anticancer Agent against Breast Cancer Stem Cells via Encapsulation in a Hyaluronan-Conjugated Liposome
Authors: Yu Ji , Shin Dae , Kim Jin-Seok
Published: November 2020

Fluvastatin (FLUVA), which is a common anti-hypercholesterolemia drug, exhibits potential anticancer activity as it suppresses the proliferation, angiogenesis, and metastasis of breast cancer cells via inhibiting 3-hydroxy-methyl glutaryl-coenzyme A (HMG-CoA) reductase. In this study, hyaluronan-conjugated FLUVA-encapsulating liposomes (HA-L-FLUVA) were evaluated for their anticancer efficacy in vitro and in vivo. The particle size, zeta potential, and encapsulation efficiency of HA-L-FLUVA were 158.36 ± 1.78 nm, −24.85 ± 6.26 mV, and 35%, respectively. Growth inhibition of breast cancer stem cells (BCSCs) by HA-L-FLUVA was more effective than that by free FLUVA. The half maximal inhibitory concentration (IC50) values of FLUVA, L-FLVUA, and HA-L-FLUVA were 0.16, 0.17, and 0.09 μM, respectively. The in vivo anticancer effect of HA-L-FLUVA in combination with doxorubicin (DOX) was more effective than that of free FLUVA, free DOX, and HA-L-FLUVA. The longest survival of mice was achieved by treatment with FLUVA (15 mg/kg) and HA-L-FLUVA (15 mg/kg) + DOX (3 mg/kg), followed by HA-L-FLUVA (15 mg/kg), Dulbecco’s phosphate buffered saline, and DOX (3 mg/kg). No more than 10% body weight loss was observed in the mice injected with FLUVA, indicating that the drug was not toxic. Taken together, these results indicate that HA-L-FLUVA could serve as an effective anticancer drug by inhibiting the growth of both breast cancer cells and cancer stem cells.
Exogenous ACC Deaminase Is Key to Improving the Performance of Pasture Legume-Rhizobial Symbioses in the Presence of a High Manganese Concentration
Authors: Paço Ana , da-Silva José Rodrigo , Torres Denise Pereira , Glick Bernard R. , Brígido Clarisse
Published: November 2020

Manganese (Mn) toxicity is a very common soil stress around the world, which is responsible for low soil fertility. This manuscript evaluates the effect of the endophytic bacterium Pseudomonas sp. Q1 on different rhizobial-legume symbioses in the absence and presence of Mn toxicity. Three legume species, Cicer arietinum (chickpea), Trifolium subterraneum (subterranean clover), and Medicago polymorpha (burr medic) were used. To evaluate the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase produced by strain Q1 in these interactions, an ACC deaminase knockout mutant of this strain was constructed and used in those trials. The Q1 strain only promoted the symbiotic performance of Rhizobium leguminosarum bv. trifolii ATCC 14480T and Ensifer meliloti ATCC 9930T, leading to an increase of the growth of their hosts in both conditions. Notably, the acdS gene disruption of strain Q1 abolished the beneficial effect of this bacterium as well as causing this mutant strain to act deleteriously in those specific symbioses. This study suggests that the addition of non-rhizobia with functional ACC deaminase may be a strategy to improve the pasture legume–rhizobial symbioses, particularly when the use of rhizobial strains alone does not yield the expected results due to their difficulty in competing with native strains or in adapting to inhibitory soil conditions.
Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen
Authors: Panis Bart , Nagel Manuela , Van den houwe Ines
Published: November 2020

The conservation of crop genetic resources, including their wild relatives, is of utmost importance for the future of mankind. Most crops produce orthodox seeds and can, therefore, be stored in seed genebanks. However, this is not an option for crops and species that produce recalcitrant (non-storable) seeds such as cacao, coffee and avocado, for crops that do not produce seeds at all; therefore, they are inevitably vegetatively propagated such as bananas, or crops that are predominantly clonally propagated as their seeds are not true to type, such as potato, cassava and many fruit trees. Field, in vitro and cryopreserved collections provide an alternative in such cases. In this paper, an overview is given on how to manage and setup a field, in vitro and cryopreserved collections, as well as advantages and associated problems taking into account the practical, financial and safety issues in the long-term. In addition, the need for identification of unique accessions and elimination of duplicates is discussed. The different conservation methods are illustrated with practical examples and experiences from national and international genebanks. Finally, the importance of establishing safe and long-term conservation methods and associated backup possibilities is highlighted in the frame of the global COVID-19 pandemic.
Characterization of Polyvinylidene Fluoride (PVDF) Electrospun Fibers Doped by Carbon Flakes
Authors: Kaspar Pavel , Sobola Dinara , Částková Klára , Knápek Alexandr , Burda Daniel , Orudzhev Farid , Dallaev Rashid , Tofel Pavel , Trčka Tomáš , Grmela Lubomír , Hadaš Zdeněk
Published: November 2020

Polyvinylidene fluoride (PVDF) is a modern polymer material used in a wide variety of ways. Thanks to its excellent resistance to chemical or thermal degradation and low reactivity, it finds use in biology, chemistry, and electronics as well. By enriching the polymer with an easily accessible and cheap variant of graphite, it is possible to affect the ratio of crystalline phases. A correlation between the ratios of crystalline phases and different properties, like dielectric constant as well as piezo- and triboelectric properties, has been found, but the relationship between them is highly complex. These changes have been observed by a number of methods from structural, chemical and electrical points of view. Results of these methods have been documented to create a basis for further research and experimentation on the usability of this combined material in more complex structures and devices.