bluuebunny/crossref_metadata_2025 huggingface dataset:
Post-Anthesis Photosynthetic Properties Provide Insights into Yield Potential of Tartary Buckwheat Cultivars
Authors: Xiang Dabing , Ma Chengrui , Song Yue , Wu Qi , Wu Xiaoyong , Sun Yanxia , Zhao Gang , Wan Yan
Published: March 2019

Photosynthesis is the basis for plant productivity, and improvement of photosynthetic efficiency is an important way to improve crop yield. However, the relationship between photosynthetic parameters and the yield of Tartary buckwheat (Fagopyrum tataricum) under rainfed conditions is unclear. A two-year field trial was conducted during 2016 and 2017 to assess the photosynthetic capacity of different leaves, dry matter accumulation, and yield of four Tartary buckwheat cultivars from flowering to maturity. The leaves of all cultivars aged gradually after flowering, and the leaf chlorophyll (Chl) and soluble protein (SP) contents, net photosynthetic rates (Pn), transpiration rates (Tr), and stomatal conductance (Gs) tended to decline. The Chl, SP, Pn, Tr, and Gs of cultivars (cvs.) XiQiao2 and QianKu3 were significantly higher than those of LiuKu3 and JiuJiang at each sampling time from 18 days after anthesis to maturity, but the intercellular CO2 content (Ci) showed the opposite trend. Cultivars XiQiao2 and QianKu3 produced more total dry matter (mean 17.1% higher), had higher harvest index (HI, mean 16.4% higher), and yield (mean 29.0% higher) than cvs. LiuKu3 and JiuJiang at maturity, and the difference was remarkably consistent. The yield of all the cultivars was positively correlated with leaf Chl, SP, Pn, Tr, and Gs, but negatively correlated with Ci. At late growth stages, the high-yielding cultivars maintained higher Chl, SP contents, Pn, Tr, and Gs, and showed higher dry matter accumulation and lower Ci than the low-yielding cultivars, consistent with their higher leaf photosynthetic capacity. The important factors determining the yield of Tartary buckwheat were maintaining higher leaf Chl and SP content and photosynthetic capacity and delaying aging during the grain formation stage. Enhanced rates of photosynthesis and dry matter accumulation led to higher post-anthesis accumulation of biomass with a positive impact on grain number and higher yield.
Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages
Authors: Li Taotao , Wang Xia , Zhang Hongyu , Chen Zhili , Zhao Xingxu , Ma Youji
Published: March 2019

BOLL is implicated in mammalian testicular function maintenance and spermatogenesis. To understand the expression patterns and biological functions of sheep BOLL, we examined the expression and immunolocalization of BOLL in the developing testes of Small-Tail Han sheep aged 0 days (D0), 2 months (2M), 5 months (5M), 1 year (1Y), and 2 years (2Y), by qPCR, Western blot, and immunohistochemistry methods. Firstly, morphological studies revealed that, in addition to spermatogonia, ordered and clear spermatocytes, as well as round and elongated spermatids and sperm, were found in the 1Y and 2Y testicular seminiferous tubules of the sheep testes, compared with the D0, 2M, and 5M testes, as analyzed by hematoxylin and eosin (H&E) staining. The diameter and area of the seminiferous tubules, epithelial thickness, and the area and perimeter of the tubule lumens gradually increased with age. BOLL was specifically expressed in testes and upregulation of BOLL transcript expression was higher in the testes of the 1Y and 2Y groups than in those of the D0, 2M, and 5M groups. Similarly, BOLL protein was expressed mainly in the 1Y and 2Y testes, ranging from primary spermatocytes to round spermatids, as well as in the spermatozoa. This study is the first demonstration that sheep BOLL might serve as a key regulator of the spermiogenesis involved in sperm maturity, in addition to its role as a crucial meiotic regulator.
Optimization of In Vitro Culture Conditions of Sturgeon Germ Cells for Purpose of Surrogate Production
Authors: Xie Xuan , Li Ping , Pšenička Martin , Ye Huan , Steinbach Christoph , Li Chuangju , Wei Qiwei
Published: March 2019

To expand germ cell populations and provide a consistent supply for transplantation, we established basal culture conditions for sturgeon germ cells and subsequently increased their mitotic activity by eliminating gonad somatic cells, supplementing with growth factor, and replacing fetal bovine serum (FBS). The initial basal culture conditions were Leibovitz’s L-15 medium (pH 8.0) supplemented with 5% FBS (p < 0.001) at 21 °C. Proliferation of germ cells was significantly enhanced and maintained for longer periods by elimination of gonad somatic cells and culture under feeder-cell free conditions, with addition of leukemia inhibitory factor and glial-cell-derived neurotrophic factor (p < 0.001). A serum-free culture medium improved germ cell proliferation compared to the L-15 with FBS (p < 0.05). Morphology remained similar to that of fresh germ cells for at least 40 d culture. Germline-specific gene expression analysis revealed no significant changes to germ cells before and after culture. Sterlet Acipenser ruthenus germ cells cultured more than 40 days showed development after transplant into Russian sturgeon Acipenser gueldenstaedtii. Polymerase chain reaction showed 33.3% of recipient gonads to contain sterlet cells after four months. This study developed optimal culture condition for sturgeon germ cells. Germ cells after 40 d culture developed in recipient gonads. This study provided useful information for culture of sturgeon germ cells.
A Mini-Review on Ceftaroline in Bacteremia Patients with Methicillin-Resistant Staphylococcus aureus (MRSA) Infections
Authors: Lounsbury Nicole , Reeber Mary G. , Mina Georges , Chbib Christiane
Published: March 2019

Objective: The objective of this review is to describe the outcomes of patients treated with ceftaroline in the non-Food and Drug Administration (FDA) approved indication of methicillin-resistant Staphylococcus aureus (MRSA) infections in both pediatric and adult populations. Data sources: A systematic overview was conducted by searching PubMed, Medline, and The Cochrane Library up to January 2019. Study selection and data extraction: All English-language clinical trials and case reports related to the efficacy of ceftaroline in new, not-yet-approved FDA indications in MRSA infections in pediatric or adult populations. Data synthesis: In the case of MRSA bacteremia (MRSAB) infections, three different randomized studies in pediatric patients showed effectiveness of ceftaroline. When used in the case of adult populations with MRSA bacteremia, a small trial of 16 patients showed 50% clinical success in patients with acute bacterial skin and skin structure infections versus 63% clinical success in patients with community-acquired bacterial pneumonia. Another case series of six refractory case reports showed 50% clinical success of ceftaroline in patients with MRSA. Conclusions: Although there are few case reports and limited data to date, ceftaroline fosamil should continue to be studied as an alternative therapy in MRSA infections in both pediatric and adult populations. Clinical success rates of ceftaroline were, in most cases, considered high when treating patients with MRSA infection. More clinical trials need to be studied. In the specific case of MRSA bacteremia, the treatment options remain few and ceftaroline should be extensively studied for the salvage treatment of MRSAB.
Investigation on Characteristics of Microwave Treatment of Organic Matter in Municipal Dewatered Sludge
Authors: Wang Huafang , Sun Jie , Xu Yuzhe , Feng Haowen , Duan Ling , He Xin
Published: March 2019

This study aimed to utilize a microwave technology to degrade active organic matters of the municipal dewatered sludge in a high-temperature environment. The effects of extraction agent, nanomaterial assistants, and microwave-absorbing agents and activating agents on the degradation efficiency were investigated. Dimethyl carbonate was used as the extraction agent. Nanostructured titanium oxide (TiO2) and zinc oxide (ZnO) exhibited effective assistance in the process of microwave treatment. We also developed a kind of microwave-absorbing agent, which was the sludge-based biological carbon. The sodium sulfate (Na2SO4), calcium hydroxide (Ca(OH)2), and magnesium chloride (MgCl2) were selected as activating agents to facilitate the organic matter discharging from the sludge. Through optimizing the experimental factors, it was confirmed that 0.1 wt% TiO2, 0.1 wt% ZnO, 2 wt% dimethyl carbonate, 10 wt% sludge-based biological carbon, 7.5 wt% Ca(OH)2, 0.5 wt% MgCl2, and 6 wt% Na2SO4 were the most appropriate addition amounts in the municipal dewatered sludge to make the organic matter decrease from 42.17% to 22.45%, and the moisture content reduce from 82.98% to 0.48% after the microwave treatment. By comparison, the organic matter degradation is almost zero, and the moisture content decreases to 8.69% without any additives. Moreover, the residual inert organic matter and sludge can be further solidified to lightweight construction materials by using liquid sodium silicate as the curing agent. The research provides a significant reference for the effective, fast, and low-cost treatment of the organic matter in the municipal sludge.
The Effect of Pharmaceutical Excipients for Applying to Spray-Dried Omega-3 Powder
Authors: Hwang Chan-Joo , Na Young-Guk , Huh Hyun , Kim MinKi , Lee Hong-Ki , Cho Cheong-Weon
Published: March 2019

Omega-3 fatty acid plays a role in protecting cells in the human body, maintaining the structure of the cell, and helping smooth metabolism. Also, it inhibits the formation of blood clotting and is effective in enhancing the formation of bone. However, the instability due to fatty acid oxidation and a fishy smell are the reasons it is avoided by people. In this study, we tried to obtain the omega-3 powder through spray-drying method using a variety of binders and surfactants for improving the limit of omega-3 fatty acid. First of all, an olive oil was used instead of omega-3 for optimization of the preparation of spray-dried omega-3 powder. Through the screening of binders and surfactants, γ-cyclodextrin and hydrogenated lecithin were chosen as a binder and a surfactant, respectively. Omega-3-loaded spray-dried powder was obtained, eventually. The morphology of omega-3-loaded spray-dried powder was spherical of 310 nm and the DHA amount was 98%. This study suggested that the transformation of omega-3 fatty acid into solid state by spray-drying using a binder and a surfactant was successively performed.
A New Hybrid Reciprocating Compressor Model Coupled with Acoustic FEM Characterization and Gas Dynamics
Authors: Liu Zhan , Lan Zhujun , Guo Jianzhang , Zhang Junmei , Xie Yushen , Cao Xing , Duan Zhenya
Published: March 2019

Accurate comprehension of thermodynamic demeanor and pressure pulsation propagation is of great attractiveness in a reciprocating compressor system. To consider the reciprocal interaction between compressor and pipelines, a hybrid numerical model is thus built by coupling the in-cylinder lumped parameter approach, in-pipe 1D gas dynamics and 3D acoustic characteristics of chambers. The transmission and reflection coefficients of a geometrically complex chamber are achieved by the definition of an acoustic characterization method based on acoustic FEM simulation data, with a high level of accuracy. Numerical results of this new hybrid model are compared with predictions from the traditional hybrid model with in-pipe 1D gas dynamics, together with experimental data. Through comparison analysis, the advantages are highlighted in using the acoustic FEM characterization for complex elements since the new model performs numerical solution without introducing any simplifications to the geometry of fluid domain.
Holographic Three-Dimensional Virtual Reality and Augmented Reality Display Based on 4K-Spatial Light Modulators
Authors: Gao Hongyue , Xu Fan , Liu Jicheng , Dai Zehang , Zhou Wen , Li Suna , Yu Yingjie , Zheng Huadong
Published: March 2019

In this paper, we propose a holographic three-dimensional (3D) head-mounted display based on 4K-spatial light modulators (SLMs). This work is to overcome the limitation of stereoscopic 3D virtual reality and augmented reality head-mounted display. We build and compare two systems using 2K and 4K SLMs with pixel pitches 8.1 μm and 3.74 μm, respectively. One is a monocular system for each eye, and the other is a binocular system using two tiled SLMs for two eyes. The viewing angle of the holographic head-mounted 3D display is enlarged from 3.8 ∘ to 16.4 ∘ by SLM tiling, which demonstrates potential applications of true 3D displays in virtual reality and augmented reality.
A Nonlinear Method for Characterizing Discrete Defects in Thick Multilayer Composites
Authors: Teng Guoyang , Zhou Xiaojun , Yang Chenlong , Zeng Xiang
Published: March 2019

Discrete defects in thick composites are difficult to detect for the small size and the structure noise that appears in multilayer composites. In this paper, a nonlinear method, called recurrence analysis, has been used for characterizing discrete defects in thick section Carbon Fiber Reinforced Polymer (CFRP) with complex lay-up. A 10 mm thick CFRP specimen with nearly zero porosity was selected, and blind holes with different diameters were artificially constructed in the specimen. The second half of the backscattered signal was analyzed by recurrence analysis for areas with or without a defect. The recurrence plot (RP) visualized the chaotic behavior of the ultrasonic pulse, and the statistical results of recurrence quantification analysis (RQA) characterized the instability of the signal and the effect of defects. The results show that the RQA variable differences are related to the size of blind holes, which give a probable detection of discrete geometric changes in thick multilayer composites.
Optimal Power Dispatch of an Offshore Wind Farm under Generator Fault
Authors: Ma Kuichao , Zhu Jiangsheng , Soltani Mohsen , Hajizadeh Amin , Chen Zhe
Published: March 2019

For offshore wind farms, the power loss caused by the wake effect is large due to the large capacity of the wind turbine. At the same time, the operating environment of the offshore wind farm is very harsh, and the cost of maintenance is higher than that of the onshore wind farm. Therefore, it is worthwhile to study through reasonable control how to reduce the wake loss of the wind farm and minimize the losses caused by the fault. In this paper, the Particle Swarm Optimization (PSO) algorithm is used to optimize the active power dispatch of wind farms under generator cooling system faults. The optimization objectives include avoiding the further deterioration of the generator fault, reducing unnecessary power loss of the faulty wind turbine, tracking the power demand from the Transmission System Operator (TSO), and reducing the power fluctuation caused by the PSO algorithm. The proposed optimal power dispatch strategy was compared with the two generally-used fault-handling methods and the proportional dispatch strategy in simulation. The result shows that the proposed strategy can improve the power generation capacity of the wind farm and achieve an efficient trade-off between power generation and fault protection.
Analysis of Seven Biogenic Amines and Two Amino Acids in Wines Using Micellar Electrokinetic Chromatography
Authors: Huang Chih-Ying , Wang Yu-Xian , Wang Xin-Zhi , Hu Cho-Chun , Chiu Tai-Chia
Published: March 2019

A low-cost, simple, and fast method utilizing micellar electrokinetic chromatography for the simultaneous determination of seven biogenic amines and two amino acids was developed. A background electrolyte containing 5 mM phosphate buffer (pH 3.7) and 20 mM sodium dodecyl sulfate was used. The optimal separation of nine investigated analytes was achieved in 11 min, with limits of detection (S/N = 3) ranging from 0.11 to 0.61 µM. The linear ranges for all analytes were observed between 0.55 and 10.0 μM (R2 > 0.990). The developed approach was extended to the analysis of analytes in commercial wine and beer samples. The recoveries of the proposed method ranged from 98.8% to 115.6%.
Inundation Risk Assessment of Underground Space Using Consequence-Probability Matrix
Authors: Han Yong-sik , Shin Eun Taek , Eum Tae Soo , Song Chang Geun
Published: March 2019

Increases in the frequency and severity of extreme rainfall might cause catastrophic submergence of underground spaces. Therefore, it is essential to predict the flood risk for proactive design. This study presents the methodology of acquiring risk level considering both flood intensity and evacuation difficulty. The flood momentum was computed by a 2D hydraulic flow model, and the flood intensity (FI) was employed to evaluate the consequence of flooding. To investigate the level of evacuation difficulty, the spatial layout of rooms, together with walking speed, were considered in the risk analysis process. If stormwater runoff enters an underground space, zones far away from the inlet usually have low risk levels. However, when the level of evacuation difficulty was considered, the risk level was dependent on the evacuation distance and location of the inlet and exit. If people are in zones with a risk level of 4 or 5, a rapid evacuation is necessary for preventing human casualties. The proposed methodology incorporated with the inundation model can be applied to any underground space regardless of the location of stairs, the number of exits, shape of rooms, or layout of the floor. Consequently, it will contribute to mitigating flood damage in an underground space.
Sustainable Agri-Food Supply Chain Performance Measurement Model for GMO and Non-GMO Using Data Envelopment Analysis Method
Authors: Saputri Virda Hersy Lutviana , Sutopo Wahyudi , Hisjam Muhammad , Ma’aram Azanizawati
Published: March 2019

The increase in food demand in Indonesia is one of the consequences of the imbalance between population growth and declining food products. One of alternative technologies that can be used in plant breeding programs to increase agricultural production, in order to meet food demands, is genetically modified organism (GMO) technology. This technology presents a lot of pros and cons among the public-related impacts that will be accepted by consumers. The purpose of this study was to determine the level of sustainability between GMO and non-GMO foods. The performance measurement model for GMO and non-GMO foods was considered according to the seven issues of sustainability that represented environmental, social, and economic aspects. The assessment method was conducted by using Adjusted Profit (AP) with Total Price Recovery (TPR) indicators and Total Factor Productivity (TFP) by utilizing the Data Envelopment Analysis (DEA) Method. Assessments made on each supply chain component included agriculture, processing, and transport to wholesalers/retailers. This study used numerical examples of rice production in Indonesia. The research results found that the performance of non-GMO rice chain is better than GMO rice. It indicates that non-GMO rice is more sustainable. The results show that the proposed model can be applied to measure the sustainability of GMO and Non-GMO agri-food supply chain performance.
The Ethics of Representation in Light of Minamata Disease: Tsuchimoto Noriaki and His Minamata Documentaries
Authors: Inoue Miyo
Published: March 2019

In this paper, I will examine how Japanese documentary filmmaker Tsuchimoto Noriaki (1928–2008) tackled the issue of visual ethics through the representation of Matsunaga Kumiko and Kamimura Tomoko—two young female patients known for the symbolic roles they each played in the history of Minamata disease. I will introduce the ethical challenge Tsuchimoto encountered upon his first visit to Minamata in 1965—especially how he grappled with the question of filming subjects (shutai) who were unconscious and/or unable to express whether they approved the act of filming or not—and how such conundrums were reflected into his representation of Kumiko in her hospital bed. For the analysis of the representation of Tomoko as seen in Tsuchimoto’s documentary, I will bring in W. Eugene Smith’s photograph “Tomoko and Mother in the Bath” as a point of comparison to explore what could be an ethical representation of Minamata disease patients, including the issue of photographs that seem to beautify the tragedy. Based on the above examinations, I will argue that the challenges Tsuchimoto faced upon representing unresponsive subjects and the very struggle to find a way to capture them as humans, not as patients or victims, altered his manner of artistic and political involvement with Minamata disease. And in the current post-Fukushima era, the issue of ethical representation that he kept exploring carries even more significance upon representing disasters.
Radiative Transition Parameters in Atomic Lanthanum from Pseudo-Relativistic Hartree–Fock and Fully Relativistic Dirac–Hartree–Fock Calculations
Authors: Gamrath Sébastien , Palmeri Patrick , Quinet Pascal
Published: March 2019

Calculated radiative transition probabilities and oscillator strengths are reported for 392 lines of neutral lanthanum (La I) atom in the spectral range from the near ultraviolet to the mid infrared. They were obtained using two different theoretical methods based on the pseudo-relativistic Hartree–Fock (HFR) and the fully relativistic multiconfiguration Dirac–Hartree–Fock (MCDHF) approaches, both including the most important intravalence and core-valence electron correlations. The quality of these radiative parameters was assessed through detailed comparisons between the results obtained using different physical models and between our theoretical results and the experimental data, where available. Of the total number of La I lines listed in the present work, about 60% have gf- and gA-values determined for the first time.
An Overview of Bioinspired and Biomimetic Self-Repairing Materials
Authors: Speck Olga , Speck Thomas
Published: March 2019

During the 3.8 billion years of biological evolution, a multitude of functional principles has been developed in all kingdoms of life enabling the sealing and healing of diverse types of damage. Inspired by this treasure trove, biologists and engineers have become increasingly interested in learning from biological insights for the development of self-repairing materials. In this review, particular attention is paid to the systematic transfer of knowledge from wound reactions in biological role models to technical applications with self-repair function. This knowledge transfer includes bioinspiration in terms of the conscious implementation of an idea from nature or biomimetics in the form of a systematic transfer of underlying functional principles found in selected biological role models. The current overview presents a selection of breakthroughs regarding bioinspired or biomimetic self-repairing materials, including the initial basic publications and the recent publications of the last eight years. Each reviewed publication is presented with reference to three key criteria: (i) self-repair mechanisms in plants or animals as role models; (ii) knowledge transfer from living nature to technology; and (iii) bioinspired or biomimetic materials with self-repair function. Finally, damage control is discussed with a focus on damage prevention and damage management.
Electrochemical Response of Saccharomyces cerevisiae Corresponds to Cell Viability upon Exposure to Dioclea reflexa Seed Extracts and Antifungal Drugs
Authors: Arthur Patrick Kobina , Yeboah Anthony Boadi , Issah Ibrahim , Balapangu Srinivasan , Kwofie Samuel K. , Asimeng Bernard O. , Foster E. Johan , Tiburu Elvis K.
Published: March 2019

Dioclea reflexa bioactive compounds have been shown to contain antioxidant properties. The extracts from the same plant are used in traditional medical practices to treat various diseases with impressive outcomes. In this study, ionic mobility in Saccharomyces cerevisiae cells in the presence of D. reflexa seed extracts was monitored using electrochemical detection methods to link cell death to ionic imbalance. Cells treated with ethanol, methanol, and water extracts were studied using cyclic voltammetry and cell counting to correlate electrochemical behavior and cell viability, respectively. The results were compared with cells treated with pore-forming Amphotericin b (Amp b), as well as Fluconazole (Flu) and the antimicrobial drug Rifampicin (Rif). The D. reflexa seed water extract (SWE) revealed higher anodic peak current with 58% cell death. Seed methanol extract (SME) and seed ethanol extract (SEE) recorded 31% and 22% cell death, respectively. Among the three control drugs, Flu revealed the highest cell death of about 64%, whereas Amp b and Rif exhibited cell deaths of 35% and 16%, respectively, after 8 h of cell growth. It was observed that similar to SWE, there was an increase in the anodic peak current in the presence of different concentrations of Amp b, which also correlated with enhanced cell death. It was concluded from this observation that Amp b and SWE might follow similar mechanisms to inhibit cell growth. Thus, the individual bioactive compounds from the water extracts of D. reflexa seeds could further be purified and tested to validate their potential therapeutic application. The strategy to link electrochemical behavior to biochemical responses could be a simple, fast, and robust screening technique for new drug targets and to understand the mechanism of action of such drugs against disease models.
Ayres Theories of Autism and Sensory Integration Revisited: What Contemporary Neuroscience Has to Say
Authors: Kilroy Emily , Aziz-Zadeh Lisa , Cermak Sharon
Published: March 2019

Abnormal sensory-based behaviors are a defining feature of autism spectrum disorders (ASD). Dr. A. Jean Ayres was the first occupational therapist to conceptualize Sensory Integration (SI) theories and therapies to address these deficits. Her work was based on neurological knowledge of the 1970’s. Since then, advancements in neuroimaging techniques make it possible to better understand the brain areas that may underlie sensory processing deficits in ASD. In this article, we explore the postulates proposed by Ayres (i.e., registration, modulation, motivation) through current neuroimaging literature. To this end, we review the neural underpinnings of sensory processing and integration in ASD by examining the literature on neurophysiological responses to sensory stimuli in individuals with ASD as well as structural and network organization using a variety of neuroimaging techniques. Many aspects of Ayres’ hypotheses about the nature of the disorder were found to be highly consistent with current literature on sensory processing in children with ASD but there are some discrepancies across various methodological techniques and ASD development. With additional characterization, neurophysiological profiles of sensory processing in ASD may serve as valuable biomarkers for diagnosis and monitoring of therapeutic interventions, such as SI therapy.
On the Role of Acoustical Improvement and Surface Morphology of Seashell Composite Panel for Interior Applications in Buildings
Authors: Setyowati Erni , Hardiman Gagoek , Purwanto  , Budihardjo Mochamad Arief
Published: March 2019

This manuscript focuses on the acoustical behaviors and surface morphology of seashell waste filler reinforced polyester (SFRP) coverings Anadara granosa Linn, Perna viridis Linn, and Placuna placenta Linn and applications in buildings. Their acoustical performances were observed using an impedance tube using a technique with two and four microphones based on ASTM E1050-98 and ASTM E2611-09. The improvements of acoustical performance were conducted by a coupled resonator inclusion with addition of a fibrous dacron layer and back cavity. The experimental results showed that the resonators and back cavity on the material structure were able to shift the absorption ability at low frequency. The promising wide broadband frequencies performance occurred when the 15 mm Placuna placenta FRP treated with front-tailed cavity without any additional fibrous layer and air gap started from 0.2 at 2.0 kHz. The combination of resonators and fibrous layer on the material structure was able to stabilize the sound transmission loss (STL) in 52–56 dB at a high frequency. On the observation of the simple surface morphology material, it was found that Placuna placenta Linn had the highest damping performances due to the smallest pores and the most carbon compound compared to the others. Therefore, this finding is very useful for building applications.
Integrated Parametric Shaping of Curvilinear Steel Bar Structures of Canopy Roofs
Authors: Dzwierzynska Jolanta
Published: March 2019

Shaping building objects is conditioned by many interrelated factors, both architectural and structural. Modern tools for shaping structures working in the environment of Rhinoceros 3D such as Grasshopper and Karamba 3D enable algorithmic-aided shaping structures, while allowing the free flow of information between the geometric model and structural model. The aim of the research is to use these tools to test the curvilinear steel bar roofs’ structures shaped based on Catalan surfaces as well as to select the most efficient structure. Three types of roof structures were analyzed: cylindroid shape, conoid shape, and hyperbolic paraboloid shape. In order to find the most preferred structural form, evolutionary structural optimization was carried out, which allowed, among others, to determine optimal discretization of the base surface, as well as optimal positions of supporting columns. As the optimization criterion, the minimum mass of the structure was assumed. The most effective structure turned out to be a structure based on hyperbolic paraboloid supported by multi-branch columns. The use of a roof with the above structure is beneficial not only because of the low weight of the structure compared to the analyzed structures, but also due to the possibility of using flat panels on the roof.
A Systematic Pan-Cancer Analysis of Genetic Heterogeneity Reveals Associations with Epigenetic Modifiers
Authors: de Matos Mafalda Ramos , Posa Ioana , Carvalho Filipa Sofia , Morais Vanessa Alexandra , Grosso Ana Rita , de Almeida Sérgio Fernandes
Published: March 2019

Intratumor genetic heterogeneity (ITH) is the main obstacle to effective cancer treatment and a major mechanism of drug resistance. It results from the continuous evolution of different clones of a tumor over time. However, the molecular features underlying the emergence of genetically-distinct subclonal cell populations remain elusive. Here, we conducted an exhaustive characterization of ITH across 2807 tumor samples from 16 cancer types. Integration of ITH scores and somatic variants detected in each tumor sample revealed that mutations in epigenetic modifier genes are associated with higher ITH levels. In particular, genes that regulate genome-wide histone and DNA methylation emerged as being determinant of high ITH. Indeed, the knockout of histone methyltransferase SETD2 or DNA methyltransferase DNMT3A using the CRISPR/Cas9 system on cancer cells led to significant expansion of genetically-distinct clones and culminated in highly heterogeneous cell populations. The ITH scores observed in knockout cells recapitulated the heterogeneity levels observed in patient tumor samples and correlated with a better mitochondrial bioenergetic performance under stress conditions. Our work provides new insights into tumor development, and discloses new drivers of ITH, which may be useful as either predictive biomarkers or therapeutic targets to improve cancer treatment.
Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres
Authors: Pettee Krista M. , Becker Kathryn N. , Alberts Arthur S. , Reinard Kevin A. , Schroeder Jason L. , Eisenmann Kathryn M.
Published: March 2019

High-grade glioma (HGG, WHO Grade III–IV) accounts for the majority of adult primary malignant brain tumors. Failure of current therapies to target invasive glioma cells partly explains the minimal survival advantages: invasive tumors lack easily-defined surgical margins, and are inherently more chemo- and radioresistant. Much work centers upon Rho GTPase-mediated glioma invasion, yet downstream Rho effector roles are poorly understood and represent potential therapeutic targets. The roles for the mammalian Diaphanous (mDia)-related formin family of Rho effectors have emerged in invasive/metastatic disease. mDias assemble linear F-actin to promote protrusive cytoskeletal structures underlying tumor cell invasion. Small molecule mDia intramimic (IMM) agonists induced mDia functional activities including F-actin polymerization. mDia agonism inhibited polarized migration in Glioblastoma (WHO Grade IV) cells in three-dimensional (3D) in vitro and rat brain slice models. Here, we evaluate whether clinically-relevant high-grade glioma patient-derived neuro-sphere invasion is sensitive to formin agonism. Surgical HGG samples were dissociated, briefly grown as monolayers, and spontaneously formed non-adherent neuro-spheres. IMM treatment dramatically inhibited HGG patient neuro-sphere invasion, both at neuro-sphere embedding and mid-invasion assay, inducing an amoeboid morphology in neuro-sphere edge cells, while inhibiting actin- and tubulin-enriched tumor microtube formation. Thus, mDia agonism effectively disrupts multiple aspects of patient-derived HGG neuro-sphere invasion.
The Role of MicroRNAs in Recurrence and Metastasis of Head and Neck Squamous Cell Carcinoma
Authors: Yang Chris X. , Sedhom Wafik , Song John , Lu Shi-Long
Published: March 2019

Head and neck squamous cell carcinoma (HNSCC) affects 650,000 people worldwide and has a dismal 50% 5-year survival rate. Recurrence and metastasis are believed the two most important factors causing this high mortality. Understanding the biological process and the underlying mechanisms of recurrence and metastasis is critical to develop novel and effective treatment, which is expected to improve patients’ survival of HNSCC. MicroRNAs are small, non-coding nucleotides that regulate gene expression at the transcriptional and post-transcriptional level. Oncogenic and tumor-suppressive microRNAs have shown to regulate nearly every step of recurrence and metastasis, ranging from migration and invasion, epithelial-mesenchymal transition (EMT), anoikis, to gain of cancer stem cell property. This review encompasses an overview of microRNAs involved in these processes. The recent advances of utilizing microRNA as biomarkers and targets for treatment, particularly on controlling recurrence and metastasis are also reviewed.
Perioperative Treatment in Resectable Gastric Cancer: Current Perspectives and Future Directions
Authors: Petrillo Angelica , Pompella Luca , Tirino Giuseppe , Pappalardo Annalisa , Laterza Maria Maddalena , Caterino Marianna , Orditura Michele , Ciardiello Fortunato , Lieto Eva , Galizia Gennaro , Castoro Carlo , De Vita Ferdinando
Published: March 2019

Gastric cancer (GC) is the fifth-most common cancer worldwide and an important cause of cancer-related-death. The growing knowledge of its molecular pathogenesis has shown that GC is not a single entity, but a constellation of different diseases, each with its own molecular and clinical characteristics. Currently, surgery represents the only curative approach for localized GC, but only 20% of patients (pts) showed resectable disease at diagnosis and, even in case of curative resection, the prognosis remains poor due to the high rate of disease relapse. In this context, multimodal perioperative approaches were developed in western and eastern countries in order to decrease relapse rates and improve survival. However, there is little consensus about the optimal treatment for non-metastatic GC. In this review, we summarize the current status and future developments of perioperative chemotherapy in resectable GC, attempting to find clear answers to the real problems in clinical practice.
Genes Located on 18q23 Are Epigenetic Markers and Have Prognostic Significance for Patients with Head and Neck Cancer
Authors: Misawa Kiyoshi , Kanazawa Takeharu , Mochizuki Daiki , Imai Atsushi , Mima Masato , Yamada Satoshi , Morita Kotaro , Misawa Yuki , Shinmura Kazuya , Mineta Hiroyuki
Published: March 2019

Loss of heterozygosity (LOH) on chromosome 18q23 is associated with significantly decreased survival in head and neck cancer. In agreement with such tumor suppressive roles, the loss of function of genes located in this region can be achieved through LOH and promotor hypermethylation. In this study, the methylation status of promoters of 18q23 genes in 243 head and neck cancer patients was assessed by quantitative methylation-specific PCR. Promoter methylation was then compared to various clinical characteristics and patient survival. GALR1 and SALL3 promoter methylation correlated with reduced disease-free survival (log-rank test, p = 0.018 and p = 0.013, respectively). Furthermore, based on multivariate Cox proportional hazards analysis, these methylation events were associated with poor disease-free survival, with hazard ratios of 1.600 (95% confidence interval: CI, 1.027–2.493; p = 0.038) and 1.911 (95% CI, 1.155–3.162; p = 0.012), respectively. By comparison, GALR1 and SALL3 methylation were not prognostic for overall survival in The Cancer Genome Atlas (TCGA) cohort. Our findings suggest that the methylation status of 18q23 genes could serve as important biomarkers for the prediction of clinical outcomes in well-annotated head and neck squamous cell carcinoma cohorts. GALR1 and SALL3 methylation could thus help to facilitate risk stratification for individualized treatment.
Photocatalytic Hydrogen Evolution Using Bi-Metallic (Ni/Pt) Na2Ti3O7 Whiskers: Effect of the Deposition Order
Authors: Garay-Rodríguez Luis F. , Murcia-López S. , Andreu T. , Moctezuma Edgar , Torres-Martínez Leticia M. , Morante J. R.
Published: March 2019

Photocatalytic hydrogen production through ethanol photo-reforming using Na2Ti3O7 whiskers increases if the sodium titanate is decorated with well-known metallic catalysts such as Ni and Pt. Whereas wet impregnation with nickel gives only a slight increase in the activity, photo-deposition of Pt increased the H2 production by more than one order of magnitude. Through the combination of both co-catalysts (Ni and Pt) a superior performance in terms of H2 production is further observed. However, hydrogen yield is largely enhanced (almost three-fold), up to 778 μmol·g−1·h−1, if the Pt is photo-deposited on the surface of the catalyst before wet impregnation with Ni species (NTO/Pt/Ni) compared to H2 yield (283 μmol·g−1·h−1) achieved with the catalyst prepared in the reverse order (NTO/Ni/Pt). Structural, morphological, optical, and chemical characterization was carried out in order to correlate physicochemical properties with their photocatalytic activity. The X-ray photoelectron spectroscopy (XPS) results show a higher concentration of Pt2+ species if this metallic layer is under the nickel oxide layer. Moreover, X-ray diffraction patterns (XRD) show that Na2Ti3O7 surface is modified for both metal decoration processes.
The Deubiquitylase USP4 Interacts with the Water Channel AQP2 to Modulate Its Apical Membrane Accumulation and Cellular Abundance
Authors: Murali Sathish K. , Aroankins Takwa S. , Moeller Hanne B. , Fenton Robert A.
Published: March 2019

Aquaporin 2 (AQP2) mediates the osmotic water permeability of the kidney collecting duct in response to arginine vasopressin (VP) and is essential for body water homeostasis. VP effects on AQP2 occur via long-term alterations in AQP2 abundance and short-term changes in AQP2 localization. Several of the effects of VP on AQP2 are dependent on AQP2 phosphorylation and ubiquitylation; post-translational modifications (PTM) that modulate AQP2 subcellular distribution and function. Although several protein kinases, phosphatases, and ubiquitin E3 ligases have been implicated in AQP2 PTM, how AQP2 is deubiquitylated or the role of deubiquitylases (DUBS) in AQP2 function is unknown. Here, we report a novel role of the ubiquitin-specific protease USP4 in modulating AQP2 function. USP4 co-localized with AQP2 in the mouse kidney, and in mpkCCD14 cells USP4 and AQP2 abundance are increased by VP. AQP2 and USP4 co-immunoprecipitated from mpkCCD14 cells and mouse kidney, and in vitro, USP4 can deubiquitylate AQP2. In mpkCCD14 cells, shRNA mediated knockdown of USP4 decreased AQP2 protein abundance, whereas no changes in AQP2 mRNA levels or VP-induced cAMP production were detected. VP-induced AQP2 membrane accumulation in knockdown cells was significantly reduced, which was associated with higher levels of ubiquitylated AQP2. AQP2 protein half-life was also significantly reduced in USP4 knockdown cells. Taken together, the data suggest that USP4 is a key regulator of AQP2 deubiquitylation and that loss of USP4 leads to increased AQP2 ubiquitylation, decreased AQP2 levels, and decreased cell surface AQP2 accumulation upon VP treatment. These studies have implications for understanding body water homeostasis.
Why is NMNAT Protective against Neuronal Cell Death and Axon Degeneration, but Inhibitory of Axon Regeneration?
Authors: Tang Bor Luen
Published: March 2019

Nicotinamide mononucleotide adenylyltransferase (NMNAT), a key enzyme for NAD+ synthesis, is well known for its activity in neuronal survival and attenuation of Wallerian degeneration. Recent investigations in invertebrate models have, however, revealed that NMNAT activity negatively impacts upon axon regeneration. Overexpression of Nmnat in laser-severed Drosophila sensory neurons reduced axon regeneration, while axon regeneration was enhanced in injured mechanosensory axons in C. elegans nmat-2 null mutants. These diametrically opposite effects of NMNAT orthologues on neuroprotection and axon regeneration appear counterintuitive as there are many examples of neuroprotective factors that also promote neurite outgrowth, and enhanced neuronal survival would logically facilitate regeneration. We suggest here that while NMNAT activity and NAD+ production activate neuroprotective mechanisms such as SIRT1-mediated deacetylation, the same mechanisms may also activate a key axonal regeneration inhibitor, namely phosphatase and tensin homolog (PTEN). SIRT1 is known to deacetylate and activate PTEN which could, in turn, suppress PI3 kinase–mTORC1-mediated induction of localized axonal protein translation, an important process that determines successful regeneration. Strategic tuning of Nmnat activity and NAD+ production in axotomized neurons may thus be necessary to promote initial survival without inhibiting subsequent regeneration.
Promethin Is a Conserved Seipin Partner Protein
Authors: Castro Inês , Eisenberg-Bord Michal , Persiani Elisa , Rochford Justin , Schuldiner Maya , Bohnert Maria
Published: March 2019

Seipin (BSCL2/SPG17) is a key factor in lipid droplet (LD) biology, and its dysfunction results in severe pathologies, including the fat storage disease Berardinelli-Seip congenital lipodystrophy type 2, as well as several neurological seipinopathies. Despite its importance for human health, the molecular role of seipin is still enigmatic. Seipin is evolutionarily conserved from yeast to humans. In yeast, seipin was recently found to cooperate with the lipid droplet organization (LDO) proteins, Ldo16 and Ldo45, two structurally-related proteins involved in LD function and identity that display remote homology to the human protein promethin/TMEM159. In this study, we show that promethin is indeed an LD-associated protein that forms a complex with seipin, and its localization to the LD surface can be modulated by seipin expression levels. We thus identify promethin as a novel seipin partner protein.
Two Birds, One Stone: Double Hits on Tumor Growth and Lymphangiogenesis by Targeting Vascular Endothelial Growth Factor Receptor 3
Authors: Hsu Ming-Chuan , Pan Mei-Ren , Hung Wen-Chun
Published: March 2019

Vascular endothelial growth factor receptor 3 (VEGFR3) has been known for its involvement in tumor-associated lymphangiogenesis and lymphatic metastasis. The VEGFR3 signaling is stimulated by its main cognate ligand, vascular endothelial growth factor C (VEGF-C), which in turn promotes tumor progression. Activation of VEGF-C/VEGFR3 signaling in lymphatic endothelial cells (LECs) was shown to enhance the proliferation of LECs and the formation of lymphatic vessels, leading to increased lymphatic metastasis of tumor cells. In the past decade, the expression and pathological roles of VEGFR3 in tumor cells have been described. Moreover, the VEGF-C/VEGFR3 axis has been implicated in regulating immune tolerance and suppression. Therefore, the inhibition of the VEGF-C/VEGFR3 axis has emerged as an important therapeutic strategy for the treatment of cancer. In this review, we discuss the current findings related to VEGF-C/VEGFR3 signaling in cancer progression and recent advances in the development of therapeutic drugs targeting VEGF-C/VEGFR3.
Multiomics and Systems Biology Are Needed to Unravel the Complex Origins of Chronic Disease
Authors: Martino David , Ben-Othman Rym , Harbeson Danny , Bosco Anthony
Published: March 2019

Modernization has now been linked to poor developmental experience, the onset of immune dysregulation and rising rates of chronic diseases in many parts of the world. Research across the epidemiological, clinical, and basic science domains supports the concept that poor developmental experience, particularly during prenatal life, can increase the risk of chronic disease, with enduring effects on long-term health. Single ‘omics’ approaches are ill-suited to dealing with the level of complexity that underpins immune dysregulation in early life. A more comprehensive systems-level view is afforded by combining multiple ‘omics’ datasets in order to delineate correlations across multiple resolutions of the genome, and of the genomes of the microorganisms that inhabit us. In this concept paper, we discuss multiomic approaches to studying immune dysregulation and highlight some of the challenges and opportunities afforded by this new domain of medical science.
Preparation of Hierarchically Structured Polystyrene Surfaces with Superhydrophobic Properties by Plasma-Assisted Fluorination
Authors: Minařík Martin , Wrzecionko Erik , Minařík Antonín , Grulich Ondřej , Smolka Petr , Musilová Lenka , Junkar Ita , Primc Gregor , Ptošková Barbora , Mozetič Miran , Mráček Aleš
Published: March 2019

The nanotexturing of microstructured polystyrene surfaces through CF4 plasma chemical fluorination is presented in this study. It is demonstrated that the parameters of a surface micropore-generation process, together with the setup of subsequent plasma-chemical modifications, allows for the creation of a long-term (weeks) surface-stable micro- and nanotexture with high hydrophobicity (water contact angle >150°). Surface micropores were generated initially via the time-sequenced dosing of mixed solvents onto a polystyrene surface (Petri dish) in a spin-coater. In the second step, tetrafluoromethane (CF4) plasma fluorination was used for the generation of a specific surface nanotexture and the modulation of the surface chemical composition. Experimental results of microscopic, goniometric, and spectroscopic measurements have shown that a single combination of phase separation methods and plasma processes enables the facile preparation of a wide spectrum of hierarchically structured surfaces differing in their wetting properties and application potentials.