bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical characteristics and changing trajectories of esophageal cancer and gastric cancer in China from 2010 to 2019: An analysis of a hospital-based database of 24,327 patients
Authors: Wang Qiang , Zhang Xiaorui , Zhong Yuxin , Wei Shijing , Li Li , Wei Wenqiang , Liu Fen , Li Yong , Wang Shaoming
Published: March 2023

Purpose

This analysis aimed to investigate the clinical characteristics and changing trajectories of gastric cancer (GC) and esophageal cancer (EC).

Methods

We collected data from a large cancer hospital in Beijing, China, from 2010 to 2019. Joinpoint regression was used to analyze the trends of histological characteristics and comorbidities.

Results

From 2010 to 2019, there were a total of 10,083 EC patients and 14,244 GC patients. Patients were mainly men and diagnosed at 55-64 years old. Metabolic comorbidity was the most common comorbidity, with hypertension being predominant. The percentages of stage I showed significant increases for EC [average annual percent change (AAPC): 10.5%] and GC (AAPC: 9.7%) patients. We also observed an increasing trend of EC and GC patients over 65 years old. For EC patients, esophageal squamous cell carcinoma (93.1%) remained as the prioritized subtype, and the middle third of the esophagus was the most common site. EC patients with three or more comorbidities increased from 0.1% to 2.2% (AAPC, 27.7%; 95% CI, 14.7% to 42.2%). For GC patients, adenocarcinoma accounts for 86.9% of the total cases, and cardia was the most common site. The ulcerative comorbidity rate decreased from 2.0% to 1.2% (AAPC, −6.1%; 95% CI, −11.6% to −0.3%).

Conclusion

ESCC remained as the prioritized histological subtype, and the middle third of the esophagus was the most common site of EC. The majority of GC patients had adenocarcinoma, and the cardia was the most common site. There was an increasing trend of patients diagnosed at stage I. These findings provide scientific evidence to guide future treatment.
Research progress of traditional Chinese medicine as sensitizer in reversing chemoresistance of colorectal cancer
Authors: Lin Xiang , Yang Xinyu , Yang Yushang , Zhang Hangbin , Huang Xuan
Published: March 2023

In recent years, the incidences and mortalities from colorectal cancer (CRC) have been increasing; therefore, there is an urgent need to discover newer drugs that enhance drug sensitivity and reverse drug tolerance in CRC treatment. With this view, the current study focuses on understanding the mechanism of CRC chemoresistance to the drug as well as exploring the potential of different traditional Chinese medicine (TCM) in restoring the sensitivity of CRC to chemotherapeutic drugs. Moreover, the mechanism involved in restoring sensitivity, such as by acting on the target of traditional chemical drugs, assisting drug activation, increasing intracellular accumulation of anticancer drugs, improving tumor microenvironment, relieving immunosuppression, and erasing reversible modification like methylation, have been thoroughly discussed. Furthermore, the effect of TCM along with anticancer drugs in reducing toxicity, increasing efficiency, mediating new ways of cell death, and effectively blocking the drug resistance mechanism has been studied. We aimed to explore the potential of TCM as a sensitizer of anti-CRC drugs for the development of a new natural, less-toxic, and highly effective sensitizer to CRC chemoresistance.
Identification of IRAK1BP1 as a candidate prognostic factor in lung adenocarcinoma
Authors: Guo Lei , Zhou Weiping , Xu Ziwei , Cao Xiaoqing , Wan Shiya , Zhang Ying Yi , Zhang Jie , Lu Hezhe
Published: March 2023

Introduction

Lung cancer is one of the major causes of cancer-related mortality worldwide. High-throughput RNA sequencing (RNA-seq) of surgically removed tumors has been used to identify new biomarkers of lung cancer; however, contamination by non-tumor cells in the tumor microenvironment significantly interferes with the search for novel biomarkers. Tumor organoids, as a pre-clinical cancer model, exhibit similar molecular characteristics with tumor samples while minimizing the interference from other cells.

Methods and Results

Here we analyzed six RNA-seq datasets collected from different organoid models, in which cells with oncogenic mutations were reprogrammed to mimic lung adenocarcinoma (LUAD) tumorigenesis. We uncovered 9 LUAD-specific biomarker genes by integrating transcriptomic data from multiple sources, and identified IRAK1BP1 as a novel predictor of LUAD disease outcome. Validation with RNA-seq and microarray data collected from multiple patient cohorts, as well as patient-derived xenograft (PDX) and lung cancer cell line models confirmed that IRAK1BP1 expression was significantly lower in tumor cells, and had no correlation with known markers oflung cancer prognosis. In addition, loss of IRAK1BP1 correlated with the group of LUAD patients with worse survival; and gene-set enrichment analysis using tumor and cell line data implicated that high IRAK1BP1 expression was associated with suppression of oncogenic pathways.

Discussion

In conclusion, we demonstrate that IRAK1BP1 is a promising biomarker of LUAD prognosis.
Ginsenosides, potential TMPRSS2 inhibitors, a trade-off between the therapeutic combination for anti-PD-1 immunotherapy and the treatment of COVID-19 infection of LUAD patients
Authors: Meng Mei , Gao Rui , Liu Zixue , Liu Fengxiang , Du Shiyu , Song Yizhi , He Jian
Published: March 2023

Background: Acting as a viral entry for coronavirus to invade human cells, TMPRSS2 has become a target for the prevention and treatment of COVID-19 infection. Before this, TMPRSS2 has presented biological functions in cancer, but the roles remain controversial and the mechanism remains unelucidated. Some chemicals have been reported to be inhibitors of TMPRSS2 and also demonstrated other pharmacological properties. At this stage, it is important to discover more new compounds targeting TMPRSS2, especially from natural products, for the prevention and treatment of COVID-19 infection.

Methods: We analyzed the correlation between TMPRSS2 expression, methylation level, overall survival rate, clinical parameters, biological process, and determined the correlation between TMPRSS2 and tumor-infiltrating lymphocytes in the tumor and adjacent normal tissue of adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) respectively by using various types of bioinformatics approaches. Moreover, we determined the correlation between TMPRSS2 protein level and the prognosis of LUAD and LUSC cohorts by immunohistochemistry assay. Furthermore, the cancer immunome atlas (TCIA) database was used to predict the relationship between the expression of TMPRSS2 and response to programmed cell death protein 1 (PD-1) blocker immunotherapy in lung cancer patients. Finally, the putative binding site of ginsenosides bound to TMPRSS2 protein was built from homology modeling to screen high-potency TMPRSS2 inhibitors.

Results: We found that TMPRSS2 recruits various types of immunocytes, including CD8+, CD4+ T cells, B cells and DCs both in LUAD and LUSC patients, and the correlation between TMPRSS2 expression and CD8+ and CD4+ T cells are stronger in LUAD rather than in LUSC, but excludes macrophages and neutrophils in LUAD patient cohorts. These might be the reason that higher mRNA and protein levels of TMPRSS2 are associated with better prognosis in LUAD cohorts rather than in LUSC cohorts. Furthermore, we found that TMPRSS2 was positively correlated with the prognosis in patient nonresponse to anti-PD-1 therapy. Therefore, we made an inference that increasing the expression level of TMPRSS2 may improve the anti-PD-1 immunotherapy efficacy. Finally, five ginsenosides candidates with high inhibition potency were screened from the natural chemical library to be used as TMPRSS2 inhibitors.

Conclusion: All these may imply that TMPRSS2 might be a novel prognostic biomarker and serve as a potential immunomodulator target of immunotherapy combination therapies in LUAD patients nonresponse to anti-PD-1 therapy. Also, these findings may suggest we should pay more attention to LUAD patients, especially those infected with COVID-19, who should avoid medicating TMPRSS2 inhibitors, such as ginsenosides to gain prophylactic and therapeutic benefits against COVID-19.
Ginaton reduces M1-polarized macrophages in hypertensive cardiac remodeling via NF-κB signaling
Authors: Wang Jie , Cai Enze , An Xiangbo , Wang Junjie
Published: March 2023

Introduction: Macrophages play a critical role in cardiac remodeling, and dysregulated macrophage polarization between the proinflammatory M1 and anti-inflammatory M2 phenotypes promotes excessive inflammation and cardiac damage. Ginaton is a natural extract extracted from Ginkgo biloba. Because of its anti-inflammatory properties, it has long been used to treat a variety of diseases. However, the role of Ginaton in modulating the diverse macrophage functional phenotypes brought on by Ang II-induced hypertension and cardiac remodeling is unknown.

Methods: In the present study, we fed C57BL/6J mice in the age of eight weeks with Ginaton (300 mg/kg/day) or PBS control, and then injected Ang II (1000 ng/kg/min) or saline for 14 days to investigate the specific efficacy of Ginaton. Systolic blood pressure was recorded, cardiac function was detected by echocardiography, and pathological changes in cardiac tissue were assessed by histological staining. Different functional phenotypes of the macrophages were assessed by immunostaining. The mRNA expression of genes was assessed by qPCR analysis. Protein levels were detected by immunoblotting.

Results: Our results showed that Ang II infusion significantly enhanced the activation and infiltration of macrophages with hypertension, cardiac insufficiency, myocardial hypertrophy, fibrosis and M1 phenotype macrophages compared with the saline group. Instead, Ginaton attenuated these effects. In addition, in vitro experiments showed that Ginaton inhibited Ang II-induced activation, adhesion and migration of M1 phenotype macrophages.

Conclusion: Our study showed that Ginaton treatment inhibits Ang II-induced M1 phenotype macrophage activation, macrophage adhesion, and mitigation, as well as the inflammatory response leading to impaired and dysfunctional hypertension and cardiac remodeling. Gianton may be a powerful treatment for heart disease.
Efficacy and safety of PD-1/PD-L1 inhibitors in the treatment of recurrent and refractory ovarian cancer: A systematic review and a meta-analysis
Authors: Zeng Siyuan , Liu Daju , Yu Yongai , Zou Lei , Jin Xianyu , Liu Bing , Liu Lifeng
Published: March 2023

Objective: To explore the efficacy and safety of PD-1/PD-L1 inhibitors in treating recurrent/refractory ovarian cancer (OC).

Methods: The online databases, including PubMed, Embase and Cochrane Library, were searched for relevant literatures on exploring the efficacy and safety of PD-1/PD-L1 inhibitors in the treatment of recurrent/refractory OC. The keywords are as follows: Ovarian neoplasms, programmed death receptor, PD-1, PD-L1, immunotherapy, and immune checkpoint inhibitor. Furthermore, qualified studies were screened for further meta-analysis.

Results: In this study, 11 studies (990 patients) were analyzed to evaluate the efficacy of PD-1/PD-L1 inhibitors in the treatment of recurrent/refractory OC. The combined results proved that the objective response rate (ORR) was 6.7%, 95% CI (4.6%,9.2%), disease control rate (DCR) was 37.9%, 95% CI (33.0%, 42.8%), median overall survival (OS) was 10.70 months, 95% CI (9.23, 12.17), and median progression free survival (PFS) was 2.24 months, 95% CI (2.05, 2.43). In addition, in terms of the safety of patients suffering from recurrent/refractory OC and receiving PD-1/PD-L1 inhibitors, the combined treatment related adverse events (TRAEs) were 70.9% (61.7%–80.2%), and the combined immune related adverse events (iAEs) were 29%, 95% CI (14.7%, 43.3%).

Conclusion: In patients with recurrent/refractory OC, PD-1/PD-L1 inhibitors were used alone and there was no obvious evidence of improved efficacy and survival. As for safety, the incidences of TRAEs and iAEs are high, so PD1/PD-L1 inhibitors should be applied according to individual conditions.

Clinical Trial Registration:https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=367525, identifier CRD42022367525.
Cortex-specific transcriptome profiling reveals upregulation of interferon-regulated genes after deeper cerebral hypoperfusion in mice
Authors: Zhang Zengyu , Guo Zimin , Tu Zhilan , Yang Hualan , Li Chao , Hu Mengting , Zhang Yuan , Jin Pengpeng , Hou Shuangxing
Published: March 2023

Background: Chronic cerebral hypoperfusion (CCH) is commonly accompanied by brain injury and glial activation. In addition to white matter lesions, the intensity of CCH greatly affects the degree of gray matter damage. However, little is understood about the underlying molecular mechanisms related to cortical lesions and glial activation following hypoperfusion. Efforts to investigate the relationship between neuropathological alternations and gene expression changes support a role for identifying novel molecular pathways by transcriptomic mechanisms.

Methods: Chronic cerebral ischemic injury model was induced by the bilateral carotid artery stenosis (BCAS) using 0.16/0.18 mm microcoils. Cerebral blood flow (CBF) was evaluated using laser speckle contrast imaging (LSCI) system. Spatial learning and memory were assessed by Morris water maze test. Histological changes were evaluated by Hematoxylin staining. Microglial activation and neuronal loss were further examined by immunofluorescence staining. Cortex-specific gene expression profiling analysis was performed in sham and BCAS mice, and then validated by quantitative RT-PCR and immunohistochemistry (IHC).

Results: In our study, compared with the sham group, the right hemisphere CBF of BCAS mice decreased to 69% and the cognitive function became impaired at 4 weeks postoperation. Besides, the BCAS mice displayed profound gray matter damage, including atrophy and thinning of the cortex, accompanied by neuronal loss and increased activated microglia. Gene set enrichment analysis (GSEA) revealed that hypoperfusion-induced upregulated genes were significantly enriched in the pathways of interferon (IFN)-regulated signaling along with neuroinflammation signaling. Ingenuity pathway analysis (IPA) predicted the importance of type I IFN signaling in regulating the CCH gene network. The obtained RNA-seq data were validated by qRT-PCR in cerebral cortex, showing consistency with the RNA-seq results. Also, IHC staining revealed elevated expression of IFN-inducible protein in cerebral cortex following BCAS-hypoperfusion.

Conclusion: Overall, the activation of IFN-mediated signaling enhanced our understanding of the neuroimmune responses induced by CCH. The upregulation of IFN-regulated genes (IRGs) might exert a critical impact on the progression of cerebral hypoperfusion. Our improved understanding of cortex-specific transcriptional profiles will be helpful to explore potential targets for CCH.
Adaptations to the stressful combination of serpentine soils and Mediterranean climate drive plant functional groups and trait richness
Authors: Hidalgo-Triana Noelia , Pérez-Latorre Andrés V. , Adomou Aristide Cossi , Rudner Michael , Thorne James H.
Published: March 2023

Introduction

Plant functional traits (FTs) are important for understanding plant ecological strategies (e.g., drought avoidance), especially in the nutrient-poor soils of serpentine ecosystems. In the Mediterranean areas, such ecosystems are characterized by climatic factors (e.g., summer drought) that exert a filtering effect.

Material and Methods

In our study, we analyzed 24 species with varying serpentine affinity, from strictly serpentine plants to generalist plants, from two ultramafic shrublands in southern Spain, considering four FTs: plant height (H), leaf area (LA), specific leaf area (SLA), and stem specific density (SSD). Additionally, we also identified the species’ dominant strategies to avoid drought and those strategies’ relationship to serpentine affinity. We used principal component analysis to identify combinations of FTs, and cluster analysis to define Functional Groups (FGs).

Results and Discussion

We defined eight FGs, which suggests that such Mediterranean serpentine shrublands are composed of species with wide-ranging of FTs. Indicator traits explained 67–72% of the variability based on four strategies: (1) lower H than in other Mediterranean ecosystems; (2) middling SSD; (3) low LA; and (4) low SLA due to thick and/or dense leaves, which contribute to long leaf survival, nutrient retention, and protection from desiccation and herbivory. Generalist plants had higher SLA than obligate serpentine plants, whereas the obligate serpentine plants showed more drought avoidance mechanisms than the generalists. Although most plant species inhabiting Mediterranean serpentine ecosystems have shown similar ecological adaptations in response to the Mediterranean environment, our results suggest that serpentine obligate plant species could present greater resilience to climate change. Given greater number and more pronounced drought avoidance mechanisms in these species compared with generalists, and the high number of FGs identified, the serpentine plants have shown adaptation to severe drought.
Characterization of the USDA Cucurbita pepo, C. moschata, and C. maxima germplasm collections
Authors: Hernandez Christopher O. , Labate Joanne , Reitsma Kathleen , Fabrizio Jack , Bao Kan , Fei Zhangjun , Grumet Rebecca , Mazourek Michael
Published: March 2023

The Cucurbita genus is home to a number of economically and culturally important species. We present the analysis of genotype data generated through genotyping-by-sequencing of the USDA germplasm collections of Cucurbita pepo, C. moschata, and C. maxima. These collections include a mixture of wild, landrace, and cultivated specimens from all over the world. Roughly 1,500 - 32,000 high-quality single nucleotide polymorphisms (SNPs) were called in each of the collections, which ranged in size from 314 to 829 accessions. Genomic analyses were conducted to characterize the diversity in each of the species. Analysis revealed extensive structure corresponding to a combination of geographical origin and morphotype/market class. Genome-wide associate studies (GWAS) were conducted using both historical and contemporary data. Signals were observed for several traits, but the strongest was for the bush (Bu) gene in C. pepo. Analysis of genomic heritability, together with population structure and GWAS results, was used to demonstrate a close alignment of seed size in C. pepo, maturity in C. moschata, and plant habit in C. maxima with genetic subgroups. These data represent a large, valuable collection of sequenced Cucurbita that can be used to direct the maintenance of genetic diversity, for developing breeding resources, and to help prioritize whole-genome re-sequencing.
Genome-wide identification and comparative analyses of key genes involved in C4 photosynthesis in five main gramineous crops
Authors: Chen Liang , Yang Yang , Zhao Zhangchen , Lu Shan , Lu Qiumei , Cui Chunge , Parry Martin A. J. , Hu Yin-Gang
Published: March 2023

Compared to C3 species, C4 plants showed higher photosynthetic capacity as well as water and nitrogen use efficiency due to the presence of the C4 photosynthetic pathway. Previous studies have shown that all genes required for the C4 photosynthetic pathway exist in the genomes of C3 species and are expressed. In this study, the genes encoding six key C4 photosynthetic pathway enzymes (β-CA, PEPC, ME, MDH, RbcS, and PPDK) in the genomes of five important gramineous crops (C4: maize, foxtail millet, and sorghum; C3: rice and wheat) were systematically identified and compared. Based on sequence characteristics and evolutionary relationships, their C4 functional gene copies were distinguished from non-photosynthetic functional gene copies. Furthermore, multiple sequence alignment revealed important sites affecting the activities of PEPC and RbcS between the C3 and C4 species. Comparisons of expression characteristics confirmed that the expression patterns of non-photosynthetic gene copies were relatively conserved among species, while C4 gene copies in C4 species acquired new tissue expression patterns during evolution. Additionally, multiple sequence features that may affect C4 gene expression and subcellular localization were found in the coding and promoter regions. Our work emphasized the diversity of the evolution of different genes in the C4 photosynthetic pathway and confirmed that the specific high expression in the leaf and appropriate intracellular distribution were the keys to the evolution of C4 photosynthesis. The results of this study will help determine the evolutionary mechanism of the C4 photosynthetic pathway in Gramineae and provide references for the transformation of C4 photosynthetic pathways in wheat, rice, and other major C3 cereal crops.
Generation of new rice germplasms with low amylose content by CRISPR/CAS9-targeted mutagenesis of the FLOURY ENDOSPERM 2 gene
Authors: Song Xiaohong , Chen Zhihui , Du Xi , Li Bin , Fei Yunyan , Tao Yajun , Wang Fangquan , Xu Yang , Li Wenqi , Wang Jun , Liang Guohua , Zhou Yong , Tan Xiaoli , Li Yulong , Yang Jie
Published: March 2023

FLOURY ENDOSPERM 2 (FLO2), encoding a tetratricopeptide repeat domain (TPR)-containing protein located in the nucleus, is considered to be a regulatory protein that controls the biosynthesis of seed storage substances. The diversity of flo2 allele is attributable for the variations in grain appearance, amylose content (AC), and physicochemical properties, influencing the eating and cooking quality (ECQ) of rice. In this study, we used CRISPR/Cas9 to introduce loss-of-function mutations into the FLOURY ENDOSPERM 2 gene in Suken118 (SK118), a widely cultivated elite japonica rice variety in Jiangsu, China. Physiochemical analyses of the flo2 mutants were congruent with previous studies, exhibiting lowered AC and viscosity, risen gel consistency (GC) and gelatinization temperature (GT) values, which were all instrumental to the improvement of ECQ. However, the wrinkled opaque appearance and the decrease in grain width, grain thickness and grain weight imply trade-offs in grain yield. Despite the ex-ante estimation for low yielding, the superior ECQ in these novel genotypes generated by using genome editing approach may have the potential for formulating high value specialty food.
Eye movements of second language learners when reading spaced and unspaced Chinese texts
Authors: Cui Yaqiong
Published: March 2023

Unlike English, Chinese does not have interword spacing in written texts, which poses difficulties for Chinese-as-a-second-language (CSL) learners’ identification of word boundaries and affects their reading comprehension and vocabulary acquisition. The eye-movement literature has suggested that interword spacing is important in alphabetic languages; examining languages that lack interword spaces such as Chinese, thus, may help to inform theoretical accounts of eye-movement control and word identification during reading. Research investigating the interword spacing effect in reading Chinese showed that adding spacing facilitated CSL learners’ reading comprehension and speed as well as vocabulary learning. However, the bulk of this research mainly looked at the learning outcomes (off-line measures), with few studies focusing on L2 learners’ reading processes. Building on this background, this study seeks to provide a descriptive perspective of the eye movements of CSL learners. In this study, 24 CSL learners with intermediate Chinese proficiency were recruited as the experimental group, and 20 Chinese native speakers were recruited as the control group. The EyeLink 1,000 eye tracker was used to record their reading of four segmentation conditions of Chinese texts, namely, no space condition, word-spaced condition, non-word-spaced condition, and pinyin-spaced condition. Results show that: (1) CSL learners with intermediate Chinese proficiency generally spent less time reading Chinese texts with spaces between words, and they showed more gazes and regressions when reading texts without spaces; (2) Non-word-spaced texts and Pinyin-spaced texts interfere with CSL learners’ reading process; and (3) Intermediate CSL learners show consistent eye movement patterns in the normal no-space condition and word-spaced condition. I conclude that word boundary information can effectively guide CSL learners’ eye movement behaviors and eye saccade planning, thus improving reading efficiency.
Investigation on sleep-related cognition of Chinese health care workers during the first wave of COVID-19 pandemic
Authors: Wang Wei , Ji Xincan , Guo Hao-Yang , Tao Mengjun , Jin Lairun , Chen Miao , Yuan Hui , Peng Hui
Published: March 2023

Background

The COVID pandemic has brought tremendous negative effects on the mental health of health care workers, such as anxiety, depression, and sleep disorders. We conducted this study to evaluate the sleep-related cognition of Chinese health care workers (HCWs) during the first wave of COVID-19 pandemic and analyze its association with sleep quality, so as to provide scientific reference for improving sleep of HCWs.

Patients and methods

A total of 404 HCWs from Yijishan Hospital of Wuhu City, China were enrolled in the study, selected by randomized cluster sampling in May 2020. We made a questionnaire to collect the general demographic information of the participants. The Pittsburgh Sleep Quality Index (PSQI) and a brief version of Dysfunctional Beliefs and Attitudes about Sleep Scale (DBAS-16) were used to measure sleep quality and sleep-related cognition, respectively.

Results

The results showed that 312 HCWs (77.2%) had false beliefs and attitudes about sleep, while only 92 HCWs (22.8%) had correct beliefs about sleep. In addition, we found that those HCWs who were older, married, with a bachelor’s degree or higher, nurses, more daily working hours (> 8 h) and monthly night shifts (≥ 5 times), had higher DBAS-16 scores (all p < 0.05). However, we did not find significant differences between men and women in DBAS-16 scores. According to the definition of PSQI, a total of 1/4 of the HCWs are poor sleepers and their DBAS-16 score was higher than good sleepers (t = 7.622, p < 0.001). In the end, we confirmed a positive correlation between sleep cognition and sleep quality (r = 0.392, p < 0.01).

Conclusion

Our study revealed false beliefs and attitudes about sleep were prevalent among HCWs during the first wave of COVID-19 pandemic, and these false beliefs about sleep were closely correlated to sleep quality. We recommend fighting against these false beliefs about sleep.
The role of substance use in the lives of incarcerated older adults: A qualitative study
Authors: Avieli Hila
Published: March 2023

Background

The prevalence of drug abuse among older adults has grown over the last decade. Despite the expanding development of a body of research dedicated to studying this phenomenon, drug abuse by incarcerated older adults has been marginalized. Thus, the aim of the present study was to explore drug abuse patterns in the lives of incarcerated older adults.

Method

Semi-structured interviews were conducted with 28 incarcerated older adults, and an interpretive analysis was used to analyze the participants’ narratives.

Findings

Four themes emerged: (1) Growing up around drugs; (2) Prison onset; (3) Professionals, and (4) Lifelong substance abuse.

Conclusion

The study findings reveal a unique typology of drug-related themes in the lives of incarcerated older adults. This typology sheds light on the interplay between aging, drug use, and incarceration and the way these three socially marginalized positions may intersect.
Electroconvulsive therapy knowledge and attitudes among patients and caregivers in South China: A preliminary study
Authors: Deng Can-Jin , Nie Sha , Mai Jian-Xin , Huang Xiong , Huang Xing-Bing , Zheng Wei
Published: March 2023

Background

Electroconvulsive therapy (ECT) is a safe and effective therapy for individuals suffering from major psychiatric disorders, but attitudes towards ECT among patients and caregivers have not been well studied. This study was conducted to elucidate patient and caregiver knowledge and attitudes concerning ECT in South China.

Methods

The sample comprised 92 patients diagnosed with major psychiatric disorders and their caregivers (n = 92). Participants completed questionnaire measures of knowledge and attitudes related to ECT.

Results

Information before ECT was inadequately provided to both caregivers and patients (55.4% versus 37.0%, p < 0.05). Caregivers reported receiving more adequate information about the therapeutic effects (50.0% versus 44.6%), side effects (67.4% versus 41.3%), and risks (55.4% versus 20.7%) of ECT when compared to patients (all p < 0.05). However, less than half of patients and caregivers believed that ECT was effective (43.5% versus 46.7%, p > 0.05), while more than half of them believed that ECT was beneficial (53.3% versus 71.7%, p < 0.05), and approximately half of them believed that ECT was safe (50.0% versus 51.1%, p > 0.05). A total of 32.6% of patients and 55.4% of caregivers (p < 0.05) reported that ECT was used only for critically ill patients. A total of 62.0% of patients experienced side effects, with memory impairment being the most commonly reported.

Conclusion

Clinicians should develop a systematic health education program before ECT treatment and ensure that patients and caregivers have an accurate understanding of ECT, particularly the treatment process, its therapeutic effects and potential side effects prior to administering this treatment.
Preferences for HIV prevention strategies among newly arrived Asian-born men who have sex with men living in Australia: A discrete choice experiment
Authors: Ung Megan , Martin Sarah , Terris-Prestholt Fern , Quaife Matthew , Tieosapjaroen Warittha , Phillips Tiffany , Lee David , Chow Eric P. F. , Medland Nick , Bavinton Benjamin R. , Pan Stephen W. , Mao Limin , Ong Jason J.
Published: March 2023

The HIV epidemic in Australia is changing with higher risk for HIV among newly-arrived Asian-born men who have sex with men (MSM) compared to Australian-born MSM. We evaluated the preferences for HIV prevention strategies among 286 Asian-born MSM living in Australia for <5 years. A latent class analysis uncovered three classes of respondents who were defined by their preferences: “PrEP” (52%), “Consistent condoms” (31%), and “No strategy” (17%). Compared to the “No strategy” class, men in the “PrEP” class were less likely to be a student or ask their partner for their HIV status. Men in the “Consistent condoms” class were more likely to get information about HIV from online, and less likely to ask their partner for their HIV status. Overall, PrEP was the preferred HIV prevention strategy for newly arrived migrants. Removing structural barriers to access PrEP can accelerate progress toward ending HIV transmission.
How perceived risk influences college students' preventive behavior: Novel data of COVID-19 campus lockdown from Wuhan, China
Authors: Zhang Lanxing , Cheng Xiaoyu , Li Zhuangzhuang
Published: March 2023

Following preventive behaviors is a key measure to protect people from infectious diseases. Protection motivation theory (PMT) suggests that perceived risk motivates individuals to take protective measures. The COVID-19 pandemic has posed unprecedented stress to the public, and changes in perceived risk may be more pronounced among college students than among other groups due to the related campus lockdown. With 1,119 college students recruited as research subjects, a quantitative research was conducted in Wuhan, China, to deduce the relationship between the perceived risk and preventive behavior of college students, as well as between the mediation effect of individual affect and the moderating effect of physical exercise. The results showed that the preventive behavior of college students was significantly affected by perceived risk, and both positive affect and negative affect played a mediating role between perceived risk and preventive behavior. Specifically, positive affect aided the relationship between perceived risk and preventive behavior, negative affect was detrimental to their relationship, and the mediation effect of positive affect is significantly higher than that of negative affect. Furthermore, physical exercise played a moderating role in the mediation effects of positive affect and negative affect. Therefore, appropriate measures should be taken to strengthen Chinese college students' perceived risk and provide them with corresponding guidance. The importance of physical exercise should also be emphasized to help college students with low perceived risk reduce negative affect, increase positive affect, and promote their preventive behavior.
Network analysis of somatic symptoms in Chinese patients with depressive disorder
Authors: Li Yang , Jia Shoumei , Cao Baohua , Chen Li , Shi Zhongying , Zhang Hao
Published: March 2023

Introduction

Network theory conceptualizes somatic symptoms as a network of individual symptoms that are interconnected and influenced by each other. In this conceptualization, the network's central symptoms have the strongest effect on other symptoms. Clinical symptoms of patients with depressive disorders are largely determined by their sociocultural context. To our knowledge, no previous study has investigated the network structure of somatic symptoms among Chinese patients with depressive disorders. The aim of this study was to characterize the somatic symptoms network structure in patients with depressive disorders in Shanghai, China.

Method

A total of 177 participants were recruited between October 2018 and June 2019. The Chinese version of the Patient Health Questionnaire-15 was used to assess somatic symptoms. In order to quantify the somatic symptom network structure, indicators of “closeness,” “strength,” and “betweenness” were employed as identifiers for network-central symptoms.

Result

The symptoms of “feeling your heart pound or race,” “shortness of breath,” and “back pain” had the highest centrality values, indicating that these symptoms were central to the somatic symptom networks. Feeling tired or mentally ill had the strongest positive correlation with insomnia or other sleep problems (r = 0.419), followed by chest pain and breathlessness (r = 0.334), back pain, and limb or joint pain (r = 0.318).

Discussion

Psychological and neurobiological research that offers insights into somatic symptoms may focus on these central symptoms as targets for treatment and future research.
The effect of job insecurity on knowledge hiding behavior: The mediation of psychological safety and the moderation of servant leadership
Authors: Jeong Jeeyoon , Kim Byung-Jik , Lee Julak
Published: March 2023

As the global economy deteriorates because of the great shocks such as COVID-19 pandemic and wars among nations, the business environment is suffered from uncertainty and risk. To deal with it, several firms have attempted to maximize its efficiency via downsizing and restructuring to diminish costs. Thus, the degree of anxiety is increased among employees who worry about the loss of their job. The current research hypothesizes that job insecurity increases employees' knowledge hiding behavior by diminishing the degree of their psychological safety. In other words, psychological safety functions as the underlying process (i.e., mediator) in the job insecurity-knowledge hiding behavior link. Furthermore, this paper tries to examine the boundary condition of how to decrease the detrimental influence of job insecurity, focusing on the moderating effect of servant leadership. Utilizing a 3-wave time-lagged data from 365 Korean employees, we empirically demonstrated that employees who perceive job insecurity are less likely to perceive psychological safety, eventually increasing their knowledge hiding behavior. We also found that servant leadership functions as a positive moderator which buffers the negative impact of job insecurity on psychological safety. Theoretical and practical contributions are described.
Mobile Application to identify and recognize emotions for children with autism: A systematic review
Authors: Al-Saadi Abdelrahman Mohammed , Al-Thani Dena
Published: March 2023

Introduction

Emotions are a vital component of human interaction. Children with Autism Spectrum Disorder (ASD) face severe difficulties in sensing and interpreting the emotions of others, as well as responding emotionally appropriately. Developers are producing many mobile applications to assist ASD children in improving their facial expression detection and reaction abilities and increasing their independence.

Objective

This systematic review aims to explore the mobile application in helping children with ASD to identify and express their feeling.

Methods

The inclusion and exclusion articles for our analysis were mapped using the PRISMA Preferred Reporting Items for Systematic Reviews and Meta-Analysis diagram. The studies were retrieved from the following four databases: Google Scholar, Scopus, Association for Computing Machinery (ACM), and Institute of Electrical and Electronics Engineers (IEEE). Additionally, two screening processes were used to determine relevant literature. Reading the title and abstract was the initial step, followed by reading the complete content. Finally, the authors display the results using a narrative synthesis.

Results

From four electronic databases, we retrieved 659 articles. six studies that met our inclusion criteria were included in the systematic review. More details about inclusion and exclusion criteria can be found in the Eligibility criteria.

Conclusion

This systematic review sheds light on current research that employed mobile applications to improve emotion detection and expression in children with ASD. This smartphone application has the potential to empower autistic children by assisting them in expressing their emotions and enhancing their ability to recognize emotions. However, it is currently deemed essential to assess the effectiveness of mobile applications for remediation through more rigorous methodological research. For example, most included studies were quantitative and focused on statical measurements. However, there is an immediate need for more incredible research in this area to include qualitative research and to consider large samples, control groups and placebo, prolonged treatment durations, and follow-up to see whether improvements are sustainable and to ensure the effectiveness of applications.
From hinterland to heartland: Knowledge and market insecurity are barriers to crop farmers using sustainable soil management in Guyana
Authors: Melville Jonathan L. , Kuznesof Sharron , Franks Jeremy R.
Published: March 2023

In Guyana, the coastal plains dominate agricultural production, while the hinterland is an emerging agricultural frontier. The coastal and hinterland regions have differing agro-climatic conditions, but share immediate climate change and environmental degradation pressures, including soil degradation. Even though climate change adaptation is prioritized over greenhouse gas mitigation in Guyana, soil-focused farming, otherwise known as sustainable soil management (SSM), can provide a system that creates synergies between these two facets of climate-smart agriculture and, also, promotes soil security. This article proposes a bottom-up planning process for SSM in Guyana by assessing its underlying psycho-social and physical facilitators and barriers. The main questions addressed are: what are the attitudes of Guyanese farmers to climate change? What are their capabilities for SSM, in terms of education, technology and government support? In answering these questions, inductive-derived thematic analysis of transcripts derived from in-depth telephone interviews with seventeen (17) farmers, from coastal and hinterland regions, provides an initial basis for ground truthing on the local appropriateness of SSM. Results show that hinterland farmers are more emotive and value-driven about their environment, while coastal farmers, instead, prioritize access to markets and gaining favorable prices for their commodities. Additionally, the lack of education and training are identified as severe limitations to the capabilities of farmers to practice SSM. In conclusion, a weak marketing environment is seen as a binding constraint of sustainable intensification as surplus goods attract low prices. Stronger linkages to dynamic markets, as well as increased investment opportunities are needed for sustainable farming to become economically feasible. Therefore, psychosocial capital must be strengthened before any natural capital is improved under Guyana's various agro-environmental policies.
Evaluation of acoustic pulse technology as a non-antibiotic therapy for bovine intramammary infections: Assessing bacterial cure vs. recovery from inflammation
Authors: Blum Shlomo Eduardo , Krifuks Oleg , Weisblith Limor , Fleker Marcelo , Lavon Yaniv , Zuckerman Alon , Hefer Yochai , Goldhor Omri , Gilad Dani , Schcolnic Tal , Leitner Gabriel
Published: March 2023

Introduction

The spread of antimicrobial resistance (AMR) is a major threat to human and animal health. Therefore, new solutions are needed to prevent returning to a world without effective antibiotics. Mastitis in dairy cows is a major reason for antimicrobial use in food animal production, and mastitis-causing bacteria have the potential to develop AMR. In this study, acoustic pulse technology (APT) was explored as an alternative to antimicrobials for the treatment of mastitis in dairy cows. APT involves the local transmission of mechanical energy through soundwaves which stimulate anti-inflammatory and angiogenic responses in the udder. These responses promote udder recovery and enhance resistance to bacterial infections.

Methods

We examined 129 Israeli dairy cows with mastitis in this prospective, controlled study to assess the efficiency of APT treatment on cure and recovery rates. An accurate diagnosis of suspected or confirmed infectious mastitis was made from cows having clinical signs of mastitis and/or somatic cell count (SCC) of above 400,000 cells/mL. The cows were divided into three groups: Group 1 (n = 29), cows with no bacterial findings (NBF); Group 2 (n = 82), cows with clinical signs of mastitis or SCC >400,000 cells/mL in the most recent test; and Group 3 (n = 18), cows with chronic mastitis (two or more tests with SCC >400,000 cells/mL within 3 months). All the cows received APT treatment, which involved 400 pulses on two sides of the infected quarter, delivered in three phases over 3 days. The cure for the mammary gland was indicated by the absence of bacterial growth in post-treatment cultures and recovery by a decrease in SCC to < 250,000 cells/mL in two of three post-treatment tests.

Results and discussion

In Group 2, cure and recovery rates were 67.1 and 64.6%, respectively, and were not significantly different between Gram-negative and Gram-positive infections. A similar recovery rate was found in NBF cows. However, in cows with chronic mastitis, both the cure and recovery rates were significantly lower (22.2 and 27.8%, respectively). These results have important implications for dairy farmers, as APT treatment could lead to substantial savings of up to $15,106/year in a 100-cow herd, considering the national estimated prevalence of mastitis and the cost of individual treatment. APT should be further investigated as a viable and sustainable alternative to antimicrobial therapy for mastitis, offering economic benefits to dairy producers and the possibility of preventing AMR.
A Cognitive Model for Technology Adoption
Authors: Sobhanmanesh Fariborz , Beheshti Amin , Nouri Nicholas , Chapparo Natalia Monje , Raj Sandya , George Richard A.
Published: March 2023

The widespread adoption of advanced technologies, such as Artificial Intelligence (AI), Machine Learning, and Robotics, is rapidly increasing across the globe. This accelerated pace of change is drastically transforming various aspects of our lives and work, resulting in what is now known as Industry 4.0. As businesses integrate these technologies into their daily operations, it significantly impacts their work tasks and required skill sets. However, the approach to technological transformation varies depending on location, industry, and organization. However, there are no published methods that can adequately forecast the adoption of technology and its impact on society. It is essential to prepare for the future impact of Industry 4.0, and this requires policymakers and business leaders to be equipped with scientifically validated models and metrics. Data-driven scenario planning and decision-making can lead to better outcomes in every area of the business, from learning and development to technology investment. However, the current literature falls short in identifying effective and globally applicable strategies to predict the adoption rate of emerging technologies. Therefore, this paper proposes a novel parametric mathematical model for predicting the adoption rate of emerging technologies through a unique data-driven pipeline. This approach utilizes global indicators for countries to predict the technology adoption curves for each country and industry. The model is thoroughly validated, and the paper outlines highly promising evaluation results. The practical implications of this proposed approach are significant because it provides policymakers and business leaders with valuable insights for decision-making and scenario planning.
Efficiency of Combed Straw Harvesting Technology Involving Straw Decomposition in the Soil
Authors: Halko Serhii , Vershkov Oleksandr , Horák Jakub , Lezhenkin Oleksandr , Boltianska Larysa , Kucher Anatolii , Suprun Olena , Miroshnyk Oleksandr , Nitsenko Vitalii
Published: March 2023

This article deals with the problem of harvesting combed straw by mixing it with the soil and the process of combed straw decomposition in particular. The idea and purpose of the research are also analysed in terms of circular economy, which represents a closed cycle. Combed straw is seen as a by-product which is reused as fertilizer to increase soil fertility, thus reducing the negative impact on the environment and increasing the efficiency of organic matter use. To analyse the qualitative aspect of the process, the introduction of an indicator is proposed—the straw decomposition coefficient. Experimental studies of straw decomposition in the soil were carried out using the mathematical theory of experimental design, where the response function is represented by the functional dependence of the straw decomposition coefficient on the length of its cutting and nitrogen and phosphorus application doses. For experimental studies, Box–Behnken design was used, which made it possible to calculate the regression coefficients by known formulas. Verification of the obtained coefficients according to Student’s t-test showed that all of them were significant. According to Fisher’s test, it was established that the model is adequate and can be used for further research. As determined by the experimental study, shredded straw incorporation improves soil properties and increases its biological activity. Ultimately, this improves plant nutrition and increases crop yields. The experiment results showed that reduced amounts of nitrogen and phosphorus fertilizers can be applied, thus leading to a reduction in the direct production costs of growing cereals in the following year. The integration of several technological processes, such as straw cutting, shredding, and incorporating it into the soil with simultaneous application of nitrogen and phosphorus fertilizers, increases the economic efficiency of grain production and a shortens the payback period for investment.