bluuebunny/crossref_metadata_2025 huggingface dataset:
New Analytical Model for Determining the Roll Pitch Diameter in Three-Roll Continuous Retained Mandrel Rolling
Authors: Wei Zhaohui , Wu Chunjing
Published: February 2023

The continuous tube-rolling method has been widely used to manufacture high-quality seamless pipes and tubes. However, the analytical model for determining the roll pitch diameter in three-roll continuous retained mandrel rolling from first principles has not yet been presented, which has, thus, hindered the development of rolling control technology in tube manufacturing. In this work, a new analytical model has been established from the force–equilibrium principles. The modelling has taken the tube-roll contact geometry, roll pressure, mandrel pull forces, inter-stand tensions, and friction coefficients into account for its formulations. Seen from the experimental results of the rolling at the plant, the maximum deviation of the predicted projected contact area is less than 6% and the maximum deviation of the calculated roll speed from the satisfactory data in field operation is less than 3.9%. The proposed model has enabled the influence of the friction coefficients on the roll pitch diameter to be quantified in theoretical analysis, and it was found that the changing amplitude of the theoretical roll pitch diameter corresponding to the commonly used data range of the friction coefficients can be above 9%. Having overcome the shortcomings of the empirical model, this model has the required prediction accuracy and flexibility for being applied to flexible tube rolling. By building the key algorithms around physical models, this modelling has advanced not only the rolling control at the plant, but also our scientific understanding of the mechanics of the continuous tube-rolling process.
The Formation Mechanism of a Multilayer-Structure Oxide Film during the Oxidation of FeCrAl in Air at 700 °C
Authors: Gao Zichen , Wang Xinyu , Zhou Danna , Wu Qingsheng , Li Chunjing , Song Liangliang , Liu Shaojun
Published: February 2023

A protective oxide film is the key to the corrosion resistance of the FeCrAl alloy. The mechanism of the formation of the multilayer oxide film of the FeCrAl alloy in 700 °C air was explored by studying the structure evolution of the oxide film and the oxidation kinetics of FeCrAl. The results show that a multilayer oxide layer is formed on the surface of the FeCrAl alloy after 1344 h, with a (Fe,Cr)2O3 layer, an Al-rich oxide layer, an Al-depleted zone, and a new Al-rich oxide layer sequentially arranged from the surface to the matrix. This indicates that the Al element plays an important role in the formation of the oxide film. The Al in the matrix is depleted to form the Al-rich oxide layer, resulting in the Al-depleted zone. The new Al-rich oxide layer is formed under the Al-depleted zone by internal oxidation. It should be noted that the precipitation of the AlN phase in the matrix is observed, which might be a probable factor for the Al-depleted zone in the matrix.
A Method to Optimize Parameters Development in L-PBF Based on Single and Multitracks Analysis: A Case Study on Inconel 718 Alloy
Authors: Giorgetti Alessandro , Baldi Niccolò , Palladino Marco , Ceccanti Filippo , Arcidiacono Gabriele , Citti Paolo
Published: February 2023

In the context of the use of AM, particularly in the L-PBF technique, the printability characterization of material occurs through the identification of its printability map as a function of printing process parameters. The printability map identifies the region where the powder melting is optimal and ensures a dense and defect-free material. Identifying the zones affected by physical phenomena that occur during the printing process which lead to material defects such as keyhole, lack of fusion and balling mode is also possible. Classical methods for the characterization of material and the identification of its printability map require the printing of a large number of specimens. The analysis of the specimens is currently time-consuming and costly. This paper proposed a methodology to identify optimal process parameters in L-PBF using an integrated single and multi-tracks analyses embedded in an overall algorithm with detailed metrics and specific factors. The main scope is to speed up the identification of printability window and, consequently, material characterization, reducing the number of micrographic analyses. The method is validated through an experimental campaign assessing the material microstructure in terms of porosity and melt pool evaluation. The case study on IN718 superalloy shows how the application of the proposed method allows an important reduction of micrographic analysis. The results obtained in the case study are a reduction of 25% for the complete definition of the printability map and more than 90% for identifying the zone with a high productivity rate.
Transcriptome and Metabolome Analysis Reveal the Flavonoid Biosynthesis Mechanism of Abelmoschus manihot L. at Different Anthesis Stages
Authors: Hou Jiaqi , Zhou Yuhan , Ran Liping , Chen Yanzhu , Zhang Ting , Sun Bowei , Yang Yimo , Sang Qianzi , Cao Li
Published: February 2023

Abelmoschus manihot L. (HSK) is a rare and endangered species in the wild that grows on the cliffs of deep mountains. As a natural plant, the chemical composition of HSK is relatively complex, which mainly includes flavonoids, organic acids, polysaccharides, and various trace elements with good effects of clearing away heat, anti-inflammatory, analgesic, and calming nerves, and inhibiting tumor cells. In this experiment, different developmental stages of HSK flowers were used for optimization of the flavonoid extraction and determining method. The antioxidant activities, flavonoid accumulation pattern, and synthesis regulatory network were analyzed using biochemistry, RNA-seq, and UPLC-MS/MS. The total content of flavonoids, vitexin rhamnoside, hyperoside, and rutin in HSK flowers at T3 stage (flower wilting) was significantly higher than in T2 (full flowering) and T1 (bud) stages. Compared with T1 and T2, the antioxidant capacity of the T3 flower alcohol extract was also the strongest, including the total reducing ability, DPPH clearance, OH clearance, O2− clearance, and total antioxidant capacity. A total of 156 flavonoids and 47,179 unigenes were detected by UPLC-MS/MS and RNA-Seq, respectively. The candidate genes and key metabolites involved in flavonoid biosynthesis were identified and the regulatory networks were also analyzed in this study. qRT-PCR test further proved that the gene expression level was consistent with the results of RNA sequence data. The relationship between the gene expression and flavonoid accumulation network provides a theoretical basis for the mining and regulation of functional genes related to the flavonoid biosynthesis and metabolism in Abelmoschus manihot L.
Callyspongia spp.: Secondary Metabolites, Pharmacological Activities, and Mechanisms
Authors: Hadisaputri Yuni Elsa , Nurhaniefah Annida Adha , Sukmara Sendi , Zuhrotun Ade , Hendriani Rini , Sopyan Iyan
Published: February 2023

One of the most widespread biotas in the sea is the sponge. Callyspongia is a sponge genus found in the seas, making it easily available. In this review, the pharmacological activity and mechanism of action of the secondary metabolites of Callyspongia spp. are addressed, which may lead to the development of new drugs and targeted therapeutic approaches. Several scientific databases, such as Google Scholar, PubMed, ResearchGate, Science Direct, Springer Link, and Wiley Online Library, were mined to obtain relevant information. In the 41 articles reviewed, Callyspongia spp. was reported to possess pharmacological activities such as cytotoxicity against cancer cell lines (36%), antifungal (10%), anti-inflammatory (10%), immunomodulatory (10%), antidiabetic and antiobesity (6%), antimicrobial (8%), antioxidant (4%), antineurodegenerative (4%), antihypercholesterolemic (2%), antihypertensive (2%), antiparasitic (2%), antiallergic (2%), antiviral (2%), antiosteoporotic (2%), and antituberculosis (2%) activities. Of these, the antioxidant, antituberculosis, and anti-inflammatory activities of Callyspongia extract were weaker compared with that of the control drugs; however, other activities, particularly cytotoxicity, show promise, and the compounds responsible may be developed into new drugs.
Unsteady Pressure-Driven Electrokinetic Slip Flow and Heat Transfer of Power-Law Fluid through a Microannulus
Authors: Deng Shuyan , Bian Ruiqing , Liang Jiacheng
Published: February 2023

To guarantee the transporting efficiency of microdevices associated with fluid transportation, mixing, or separation and to promote the heat transfer performance of heat exchangers in microelectronics, the hydrodynamic behaviors at unsteady and steady states, as well as the thermal characteristics at the steady state in a pressure-driven electrokinetic slip flow of power-law fluid in a microannulus are studied. To present a more reliable prediction, the slip phenomenon at walls and nonlinear rheology of liquid are incorporated. The modified Cauchy momentum equation applicable to all time scales and energy equations, are analytically solved in the limiting case of a Newtonian fluid and numerically solved for power-law fluids. The transient velocity profile, time evolution of flow rate, temperature profile, and heat transfer rate are computed at different flow behavior indices, electrokinetic width, slip lengths, and Brinkman numbers, thereby, the coupling effect of nonlinear rheology, slip hydrodynamics, and annular geometry on flow and thermal behaviors is explored. The unsteady flow takes a longer time to achieve the steady state for shear thinning fluids or greater slip lengths. The flow behavior index and slip length play a significant role in the flow rate and heat transfer performance. The relevant discussion can serve as a theoretical guide for the operation and thermal management of annular geometry-related flow actuation systems.
Production of Lipid Constructs by Design via Three-Dimensional Nanoprinting
Authors: Huang Yuqi , Karsai Arpad , Sambre Pallavi D. , Su Wan-Chih , Faller Roland , Parikh Atul N. , Liu Gang-yu
Published: February 2023

Atomic force microscopy (AFM) in conjunction with microfluidic delivery was utilized to produce three-dimensional (3D) lipid structures following a custom design. While AFM is well-known for its spatial precision in imaging and 2D nanolithography, the development of AFM-based nanotechnology into 3D nanoprinting requires overcoming the technical challenges of controlling material delivery and interlayer registry. This work demonstrates the concept of 3D nanoprinting of amphiphilic molecules such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Various formulations of POPC solutions were tested to achieve point, line, and layer-by-layer material delivery. The produced structures include nanometer-thick disks, long linear spherical caps, stacking grids, and organizational chiral architectures. The POPC molecules formed stacking bilayers in these constructions, as revealed by high-resolution structural characterizations. The 3D printing reached nanometer spatial precision over a range of 0.5 mm. The outcomes reveal the promising potential of our designed technology and methodology in the production of 3D structures from nanometer to continuum, opening opportunities in biomaterial sciences and engineering, such as in the production of 3D nanodevices, chiral nanosensors, and scaffolds for tissue engineering and regeneration.
Liposome Deformation Induced by Membrane-Binding Peptides
Authors: Izumi Kayano , Saito Chihiro , Kawano Ryuji
Published: February 2023

This paper presents an investigation of liposome deformation and shape distortion using four membrane-binding peptides: TAT and C105Y as cell-penetrating peptides (CPPs), and melittin and ovispirin as antimicrobial peptides (AMPs). Liposome deformation was monitored utilizing fluorescent microscopy, while the binding of peptides to the DOPC membrane was estimated through capacitance measurements. The degree of liposome deformation and shape distortion was found to be higher for the CPPs compared to the AMPs. Additionally, it was observed that C105Y did not induce liposome rupture, unlike the other three peptides. We propose that these variations in liposome distortion may be attributed to differences in secondary structure, specifically the presence of an α-helix or random coil. Our studies offer insight into the use of peptides to elicit control of liposome architecture and may offer a promising approach for regulating the bodies of liposomal molecular robots.
Epsilon-Caprolactam- and Nylon Oligomer-Degrading Bacterium Brevibacterium epidermidis BS3: Characterization and Potential Use in Bioremediation
Authors: Esikova Tatiana Z. , Akatova Ekaterina V. , Solyanikova Inna P.
Published: February 2023

epsilon-Caprolactam (Caprolactam, CAP), a monomer of the synthetic non-degradable polymer nylon-6, is the major wastewater component in the production of caprolactam and nylon-6. Biological treatment of CAP, using microbes could be a potent alternative to the current waste utilization techniques. This work focuses on the characterization and potential use of caprolactam-degrading bacterial strain BS3 isolated from soils polluted by CAP production wastes. The strain was identified as Brevibacterium epidermidis based on the studies of its morphological, physiological, and biochemical properties and 16S rRNA gene sequence analysis. This study is the first to report the ability of Brevibacterium to utilize CAP. Strain BS3 is an alcalo- and halotolerant organism, that grows within a broad range of CAP concentrations, from 0.5 up to 22.0 g/L, optimally at 1.0–2.0 g/L. A caprolactam biodegradation experiment using gas chromatography showed BS3 to degrade 1.0 g/L CAP over 160 h. In contrast to earlier characterized narrow-specific CAP-degrading bacteria, strain BS3 is also capable of utilizing linear nylon oligomers (oligomers of 6-aminohexanoic acid), CAP polymerization by-products, as sole sources of carbon and energy. The broad range of utilized toxic pollutants, the tolerance for high CAP concentrations, as well as the physiological properties of B. epidermidis BS3, determine the prospects of its use for the biological cleanup of CAP and nylon-6 production wastes that contain CAP, 6-aminohexanoic acid, and low molecular weight oligomer fractions.
Phylodynamic Analysis Suggests That Deer Species May Be a True Reservoir for Hepatitis E Virus Genotypes 3 and 4
Authors: Karlsen Anastasia A. , Kichatova Vera S. , Kyuregyan Karen K. , Mikhailov Mikhail I.
Published: February 2023

Hepatitis E virus (HEV) genotypes 3 and 4 (HEV-3 and HEV-4) cause zoonotic infection in humans, with domestic pigs and wild boars being the main reservoirs of infection. Other than suids, HEV-3 and HEV-4 are found in ruminants, most frequently in deer species. However, it is still debatable, whether HEV infection in deer is a spillover, or indicates a stable virus circulation in these host species. To explore the patterns of HEV-3 and HEV-4 transmission in deer and other host species, we performed a Bayesian analysis of HEV sequences available in GenBank. A total of 27 HEV sequences from different deer species were found in GenBank. Sequences from wild boars collected in the same territories, as well as sequences from all mammals that were most similar to sequences from deer in blast search, were added to the dataset, comprising 617 in total sequences. Due to the presence of partial genomic sequences, they were divided into four subsets (two ORF1 fragments and two ORF2 fragments) and analyzed separately. European HEV-3 sequences and Asian HEV-4 sequences collected from deer species demonstrated two transmission patterns. The first pattern was spillover infection, and the second pattern was deer-to-deer transmission, indicating stable HEV circulation in these species. However, all geographic HEV clusters that contained both deer and swine sequences originated from ancestral swine strains. HEV-3 and HEV-4 transmission patterns in ungulates reconstructed by means of Bayesian analysis indicate that deer species are a true host for HEV. However, wild and domestic swine are often the primary source of infection for ruminants living in the same areas. Complete HEV genomic sequences from different parts of the world are crucial for further understanding the HEV-3 and HEV-4 circulation patterns in wildlife.
Molecular Simulation Study on Methane Adsorption in Amorphous Shale Structure
Authors: Mohd Aji Aminah Qayyimah , Mohshim Dzeti Farhah , Maulianda Belladonna , El-Raeis Khaled Abdalla
Published: February 2023

Gas adsorption in the porous shale matrix is critical for gas-in-place (GIP) evaluation and exploration. Adsorption investigations benefit significantly from the use of molecular simulation. However, modelling adsorption in a realistic shale topology remains a constraint, and there is a need to study the adsorption behaviour using molecular models containing both organic and inorganic nanopores. Most simulations use a single component, either kerogen (organic composition) and quartz or clay (inorganic composition), to represent the shale surface. In this work, the molecular dynamic (MD) and grand conical Monte Carlo (GCMC) simulations were utilised to provide insight into methane adsorption behaviour. Amorphous shale structures composed of kerogen and quartz were constructed. The kerogen content was varied to replicate the shale with 2 wt.% and 5 wt.% Total Organic Carbon (TOC) content with 5 nm pore size. The simulated densities of the shale structures showed consistent values with actual shale from the Montney, Antrim, and Eagle Ford formations, with 2.52 g/cm3 and 2.44 g/cm3, respectively. The Average Error Analysis (ARE) was used to assess the applicability of the proposed amorphous shale model to replicate the laboratory adsorption isotherm measurements of actual shale. The ARE function showed that the amorphous shale shows good agreement with experimental measurements of all Barnett shale samples with an average of 5.0% error and slightly higher for the Haynesville samples with 8.0% error. The differences between the experimental adsorption measurement and simulation resulted from the amorphous packing, and actual shales have more minerals than the simulated model.
Structural and Geochemical Assessment of the Coralline Alga Tethysphytum antarcticum from Terra Nova Bay, Ross Sea, Antarctica
Authors: López Correa Matthias , Teichert Sebastian , Ragazzola Federica , Cazorla Vázquez Salvador , Engel Felix B. , Hurle Katrin , Mazzoli Claudio , Kuklinski Piotr , Raiteri Giancarlo , Lombardi Chiara
Published: February 2023

Crustose coralline algae (CCA) occur from the tropics to the poles in photic benthic environments. Here, we report on some of the world’s southernmost and coldest CCA sites in Terra Nova, Ross Sea, Antarctica at 74°41′ S. The recently described red alga Tethysphytum antarticum is investigated for its skeletal architecture, its mineralogical and geochemical composition, as well as for its taxonomic classification. A phylogenetic analysis based on molecular genetics and the sequencing of the photosystem II protein D1 (psbA) gave a perfect match with T. antarcticum. Histological sections and micro-CT-scans provide new diagnostic details for the conceptacles (the reproductive organs of the alga). X-ray diffractometry and electron-microprobe measurements yielded a clear high-Mg calcite (~8 mol%) composition of the skeletal parts. Detailed back-scattered electron imaging of polished petrographic thin sections revealed a two-layered thallus (vegetative plant tissue), comprising an organic-rich irregularly calcified basal layer with rectangular cells, overlain by the main thallus. Elemental maps show relatively increased sulphur in the basal layer, clearly tied to organic cell walls. MgCO3 and SrCO3 were targeted with semiquantitative elemental mappings and in an ontogenetic quantitative spot transect. Compared with temperature (−1.95 °C to +1.08 °C), the MgCO3 (mol%) reflects this world’s coldest CCA site temperature with the lowest MgCO3 content of 7.9 ± 1.6 mol%. The along transect variability, however, shows with ~6 mol% a larger MgCO3 variability than expected for the 3 °C intra-annual temperature amplitude in Terra Nova Bay. This implies that in low amplitude settings the biomineralisation control on Mg/Ca ratios can outcompete its temperature sensitivity. Mark-recapture studies, next to the environmental logger station La Zecca are suggested, to perform a detailed growth rate and biomineralisation quantification.
Predicting the Release and Migration of Potentially Harmful Elements (PHEs) during the Lightweight Ceramsite Preparation from Carbide Slag
Authors: Jiang Qi , He Yongmei , Wu Yonglin , Li Tianguo , Li Chengxue , Liu Hongpan , Wang Zhonghua , Jiang Ming
Published: February 2023

When preparing lightweight ceramsite using carbide slag, trace amounts of toxic elements are released into the atmosphere due to high-temperature calcination, posing a significant risk to the environment. The real-time monitoring of the released gases is challenging under laboratory conditions while preparing large quantities of ceramsite. Therefore, heating was simulated using experimental data and the FactSage 7.0 thermochemical database to study the release of harmful Al-, C-, H-, S-, and F-containing elements when using carbide slag to prepare lightweight ceramsite. The results indicated that no Al, C, H, S, or F elements were evident in the high-temperature liquid products obtained in a 50 °C to 1150 °C calcination temperature range. Al was present in a solid state with no gaseous products. When the temperature reached 450 °C, CO gas was released and its level increased as the temperature rose. H and S mainly combined into H2S gas, starting at 250 °C and reaching a peak at 1050 °C. H and F primarily combined into HF, starting at 400 °C. Other F-containing gases mainly included SiF4 and TiF3, which began to release at 800 °C and 900 °C, respectively. The release trends of HF, SiF4, and TiF3 were consistent with those of CO. This study aimed to conduct an environmental impact and management assessment for the preparation of lightweight ceramsite using carbide slag. The use of raw material carbide slag for the low-cost treatment of tail gas was proposed, which provides theoretical and up-to-date support for greening the application of the process.
The Effect of Iron- and Calcium-Rich Waste Rock’s Acid Baking Conditions on the Rare-Earth Extraction
Authors: Gontijo Vitor L. , Teixeira Leandro Augusto Viana , Ciminelli Virgínia Sampaio Teixeira
Published: February 2023

The work investigates the effect of sulfuric acid baking on rare earth element (REE) extraction from two waste rock samples from a phosphate mine. The role of different mineralogical assemblages and the degree of alteration (i.e., weathering), and the behavior of the main impurities–iron, phosphorus, and calcium–on REE extraction are emphasized. For both samples, the sulfuric acid baking at 25 °C, during 15 min, H2SO4:sample (w/w) mass ratio of 0.45:1 is the best condition for achieving the selective leaching of REE. For the iron rich-sample, the increase in temperature reduces REE extraction and increases iron dissolution. The corresponding sulfuric acid consumed by goethite (α-FeOOH) from 25 °C to 160 °C acid baking is ten times higher than that required for the monazite (REEPO4) reaction. Conversely, higher REE and lower iron extraction are observed by increasing (sulfuric acid/sample) mass ratio (0.95:1). Due to the high sulfuric concentration during dissolution, a local saturation zone close to the dissolution front caused the precipitation of iron oxyhydroxides. The calcium-rich sample shows lower REE extraction by leaching (63% maximum) mainly due to the entrapment of REE-bearing minerals by a gypsum layer, and lanthanide’s uptake by calcium sulfate compounds formed during leaching. The results were discussed with the help of a detailed characterization of the residues.
Study on the Effect of Different Viscosity Reducers on Viscosity Reduction and Emulsification with Daqing Crude Oil
Authors: Zhang Fan , Zhang Qun , Zhou Zhaohui , Sun Lingling , Zhou Yawen
Published: February 2023

The urgent problem to be solved in heavy oil exploitation is to reduce viscosity and improve fluidity. Emulsification and viscosity reduction technology has been paid more and more attention and its developments applied. This paper studied the viscosity reduction performance of three types of viscosity reducers and obtained good results. The viscosity reduction rate, interfacial tension, and emulsification performance of three types of viscosity reducers including anionic sulfonate, non-ionic (polyether and amine oxide), and amphoteric betaine were compared with Daqing crude oil. The results showed that the viscosity reduction rate of petroleum sulfonate and betaine was 75–85%. The viscosity reduction rate increased as viscosity reducer concentration increased. An increase in the oil–water ratio and polymer decreased viscosity reduction. When the concentration of erucamide oxide was 0.2%, the ultra-low interfacial tension was 4.41 × 10−3 mN/m. When the oil–water ratio was 1:1, the maximum water separation rates of five viscosity reducers were different. With an increase in the oil–water ratio, the emulsion changed from o/w emulsion to w/o emulsion, and the stability was better. Erucamide oxide and erucic betaine had good viscosity reduction and emulsification effects on Daqing crude oil. This work can enrich knowledge of the viscosity reduction of heavy oil systems with low relative viscosity and enrich the application of viscosity reducer varieties.
Anti-Photoaging Effect of Phaseolus angularis L. Extract on UVB-Exposed HaCaT Keratinocytes and Possibilities as Cosmetic Materials
Authors: Oh Sarang , Zheng Shengdao , Fang Minzhe , Kim Myeongju , Bellere Arce Defeo , Jeong Jeehaeng , Yi Tae-Hoo
Published: February 2023

Phaseolus angularis L. is widely cultivated and is considered a superfood because of its nutritious protein and starch contents. Nevertheless, P. angularis’s effects on skin photoaging are unknown. The aim of this study was to research the effects of P. angularis seed extract (PASE) on photoaging in human keratinocytes (HaCaT) damaged by UVB radiation so as to find out whether PASE can be used as an effective anti-photoaging ingredient in cosmetic products. The antioxidant activities were assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging, and reactive oxygen species (ROS) assays. Enzyme-linked immunosorbent assay (ELISA) analysis was used to determine the change in matrix metalloproteinase (MMP)-1, and MMP-3. The protein levels of mitogen-activated protein kinase (MAPK)/activator protein (AP)-1, transforming growth factor beta (TGF)-β/suppressor of mothers against decapentaplegic (Smad), and NF-E2-related factor (Nrf)2/antioxidant response element (ARE) were measured by western blot. As a result, PASE increased DPPH and ABTS antioxidant activities in a dose-dependent manner. Additionally, PASE treatment (100 µg/mL) significantly reverted the damage induced by UVB (125 mJ/cm2) irradiation by downregulating ROS, matrix metalloproteinase (MMP)-1, and MMP-3 secretion and expression and increasing procollagen type I production. To suppress MMP-1 and MMP-3 secretion, PASE significantly decreased UVB-induced p38 and JNK phosphorylation and phosphorylated c-Fos and c-Jun nuclear translocation. PASE promoted collagen I production by inhibiting UVB-induced TGF-β activation and Smad7 overexpression; antioxidant properties also arose from the stimulation of the Nrf2-dependent expression of the antioxidant enzymes heme oxygenase (HO)-1 and quinone oxidoreductase (NQO)-1. Our data demonstrated that PASE has the potential to prevent ROS formation induced by UVB exposure by targeting specific pathways. Thus, PASE might be a potent anti-photoaging component to exploit in developing anti-aging products.
Authentication and Geographical Characterisation of Italian Grape Musts through Glucose and Fructose Carbon Isotopic Ratios Determined by LC-IRMS
Authors: Perini Matteo , Pianezze Silvia , Guardini Katia , Allari Letizia , Larcher Roberto
Published: February 2023

The authenticity of grape musts is normally checked through a time-consuming stable isotopic analysis of carbon (δ13C) after fermentation and distillation by following the official OIV MA AS-312-06 method. In this study, the alternative use of a technique based on δ13C isotopic analysis of the major sugars of the grape must by liquid chromatography coupled with isotope ratio mass spectrometry (LC-IRMS) is provided. It allows not only the detection of the fraudulent addition to grape must of exogenous glucose and fructose deriving from C4 plants but also the characterisation of it based on its geographical origin. In order to discriminate between musts from different areas of Italy, a preliminary dataset was considered; the δ13C isotopic ratios of glucose and fructose of around 100 authentic samples were analysed. The two analysed parameters, ranging from −29.8‰ to −21.9‰, are well correlated (R2 = 0.7802) and the northern regions showed significantly more negative δ13C values for both sugars than the rest of the dataset.
Towards Antibiotic Synthesis in Continuous-Flow Processes
Authors: Comito Marziale , Monguzzi Riccardo , Tagliapietra Silvia , Palmisano Giovanni , Cravotto Giancarlo
Published: February 2023

Continuous-flow chemistry has become a mainstream process and a notable trend among emerging technologies for drug synthesis. It is routinely used in academic and industrial laboratories to generate a wide variety of molecules and building blocks. The advantages it provides, in terms of safety, speed, cost efficiency and small-equipment footprint compared to analog batch processes, have been known for some time. What has become even more important in recent years is its compliance with the quality objectives that are required by drug-development protocols that integrate inline analysis and purification tools. There can be no doubt that worldwide government agencies have strongly encouraged the study and implementation of this innovative, sustainable and environmentally friendly technology. In this brief review, we list and evaluate the development and applications of continuous-flow processes for antibiotic synthesis. This work spans the period of 2012–2022 and highlights the main cases in which either active ingredients or their intermediates were produced under continuous flow. We hope that this manuscript will provide an overview of the field and a starting point for a deeper understanding of the impact of flow chemistry on the broad panorama of antibiotic synthesis.
In Vitro Binding Effects of the Ecdysone Receptor−Binding Domain and PonA in Plutella xylostella
Authors: Feng Yanjiao , Cui Jialin , Jin Binyan , Li Xiuzhen , Zhang Xiaoming , Liu Libing , Zhang Li
Published: February 2023

Both insect ecdysone receptors and ultraspiracle belong to the nuclear receptor family. They form a nanoscale self-assembling complex with ecdysteroids in cells, transit into the nucleus, bind with genes to initiate transcription, and perform specific biological functions to regulate the molting, metamorphosis, and growth processes of insects. Therefore, this complex is an important target for the development of eco-friendly insecticides. The diamondback moth (Plutella xylostella) is a devastating pest of cruciferous vegetable crops, wreaking havoc worldwide and causing severe economic losses, and this pest has developed resistance to most chemical insecticides. In this study, highly pure EcR and USP functional domains were obtained by constructing a prokaryotic expression system for the diamondback moth EcR and USP functional domain genes, and the differences between EcR and USP binding domain monomers and dimers were analyzed using transmission electron microscopy and zeta potential. Radioisotope experiments further confirmed that the binding affinity of PonA to the EcR/USP dimer was enhanced approximately 20-fold compared with the binding affinity to the PxGST−EcR monomer. The differences between PonA and tebufenozide in binding with EcR/USP were examined. Molecular simulations showed that the hydrogen bonding network formed by Glu307 and Arg382 on the EcR/USP dimer was a key factor in the affinity enhancement. This study provides a rapid and sensitive method for screening ecdysone agonists for ecdysone receptor studies in vitro.
The Stability of a Mixed-Phase Barium Cerium Iron Oxide under Reducing Conditions in the Presence of Hydrogen
Authors: Rosen Benjamin , Sohlberg Karl
Published: February 2023

Metal oxide perovskite materials show promise for use as hydrogen separation membranes, but metal oxides can dehydrate in the presence of hydrogen to the point of decomposition. The stability of a material in the presence of hydrogen is necessary for an effective hydrogen separation membrane. The stability of a mixed phase metal oxide perovskite (BaCe0.85Fe0.15O3-δ-BaCe0.15Fe0.85O3-δ) was investigated using first-principles thermodynamics calculations based on density functional theory to examine the possible reduction processes on the surface of the material. It was found that for either phase of the material, the loss of H2 becomes thermodynamically favorable over the formation of oxygen vacancies once oxygen vacancy defects exist on the surface. Additionally, both phases of the material become more stable with respect to the dehydration or loss of oxygen with increasing concentrations of surface oxygen vacancies. Under the conditions of commercial hydrogen production (~400–1100 K), it is more thermodynamically favorable for H2 to desorb from the BaCe0.85Fe0.15O3-δ phase. Examination of the atomic-scale structure indicates that the degree of coordination of surface metal atoms in this material may control the stability of the material in reducing environments.
Experimental FTIR-MI and Theoretical Studies of Isocyanic Acid Aggregates
Authors: Krupa Justyna , Wierzejewska Maria , Lundell Jan
Published: February 2023

Homoaggregates of isocyanic acid (HNCO) were studied using FTIR spectroscopy combined with a low-temperature matrix isolation technique and quantum chemical calculations. Computationally, the structures of the HNCO dimers and trimers were optimized at the MP2, B3LYPD3 and B2PLYPD3 levels of theory employing the 6-311++G(3df,3pd) basis set. Topological analysis of the electron density (AIM) was used to identify the type of non-covalent interactions in the studied aggregates. Five stable minima were located on the potential energy surface for (HNCO)2, and nine were located on the potential energy surface for (HNCO)3. The most stable dimer (D1) involves a weak, almost linear N-H⋯N hydrogen bond. Other structures are bound by a N-H⋯O hydrogen bond or by O⋯C or N⋯N van der Waals interactions. Similar types of interactions as in (HNCO)2 were found in the case of HNCO trimers. Among nine stable (HNCO)3 structures, five represent cyclic forms. The most stable T1 trimer structure is characterized by a six-membered ring formed by three N-H⋯N hydrogen bonds and representing high symmetry (C3h). The analysis of the HNCO/Ar spectra after deposition indicates that the N-H⋯O hydrogen-bonded dimers are especially prevalent. Upon annealing, HNCO trimers were observed as well. Identification of the experimentally observed species relied on previous experimental data on HNCO complexes as well as computed data on HNCO homoaggregates’ vibrational spectra.
Rapid Uniformity Analysis of Fully Printed SWCNT-Based Thin Film Transistor Arrays via Roll-to-Roll Gravure Process
Authors: Choi Yunhyok , Jung Younsu , Song Reem , Park Jinhwa , Parajuli Sajjan , Shrestha Sagar , Cho Gyoujin , Kim Byung-Sung
Published: February 2023

The roll-to-roll (R2R) gravure process has the potential for manufacturing single-wall carbon nanotubes (SWCNT)-based thin film transistor (TFT) arrays on a flexible plastic substrate. A significant hurdle toward the commercialization of the R2R-printed SWCNT-TFT array is the lack of a suitable, simple, and rapid method for measuring the uniformity of printed products. We developed a probing instrument for characterizing R2R gravure printed TFT, named PICR2R-TFT, for rapidly characterizing R2R-printed SWCNT-TFT array that can present a geographical distribution profile to pinpoint the failed devices in an SWCNT-TFT array. Using the newly developed PICR2R-TFT instrument, the current–voltage characteristics of the fabricated SWCNT-TFT devices could be correlated to various R2R-printing process parameters, such as channel length, roll printing length, and printing speed. Thus, by introducing a characterization tool that is reliable and fast, one can quickly optimize the R2R gravure printing conditions to enhance product uniformity, thereby maximizing the yield of printed SWCNT-TFT arrays.
Recent Advances in the Spintronic Application of Carbon-Based Nanomaterials
Authors: Pawar Shweta , Duadi Hamootal , Fixler Dror
Published: February 2023

The term “carbon-based spintronics” mostly refers to the spin applications in carbon materials such as graphene, fullerene, carbon nitride, and carbon nanotubes. Carbon-based spintronics and their devices have undergone extraordinary development recently. The causes of spin relaxation and the characteristics of spin transport in carbon materials, namely for graphene and carbon nanotubes, have been the subject of several theoretical and experimental studies. This article gives a summary of the present state of research and technological advancements for spintronic applications in carbon-based materials. We discuss the benefits and challenges of several spin-enabled, carbon-based applications. The advantages include the fact that they are significantly less volatile than charge-based electronics. The challenge is in being able to scale up to mass production.
Laser-Induced Ion Formation and Electron Emission from a Nanostructured Gold Surface at Laser Fluence below the Threshold for Plasma Formation
Authors: Pento Andrey , Kuzmin Ilya , Kozlovskiy Viacheslav , Li Lei , Laptinskaya Polina , Simanovsky Yaroslav , Sartakov Boris , Nikiforov Sergey
Published: February 2023

The laser formation of positive and negative ions on a nanostructured metal surface is observed at laser fluence below the plasma formation threshold. The laser radiation energy dependences of the yield of positive and negative Au ions and charged clusters as well as electrons from the laser-induced nanostructures on the surface of gold are obtained at laser fluence below the plasma formation threshold using a pulsed laser with a wavelength of 355 nm and a pulse duration of 0.37 ns. It is shown that the ratio of the signals of positive and negative ions is constant over the entire range of the laser radiation energies, while the ion signal dependence on the laser radiation energy is described by a power function with an exponent of 9. The role of gold nanoparticles with a size of less than 5 nm in the formation of Au ions and charged Au clusters is discussed.
Hydrocarbon Sorption in Flexible MOFs—Part II: Understanding Adsorption Kinetics
Authors: Preißler-Kurzhöfer Hannes , Kolesnikov Andrei , Lange Marcus , Möllmer Jens , Erhart Oliver , Kobalz Merten , Hwang Seungtaik , Chmelik Christian , Krautscheid Harald , Gläser Roger
Published: February 2023

The rate of sorption of n-butane on the structurally flexible metal-organic framework [Cu2(H-Me-trz-ia)2], including its complete structural transition between a narrow-pore phase and a large-pore phase, was studied by sorption gravimetry, IR spectroscopy, and powder X-ray diffraction at close to ambient temperature (283, 298, and 313 K). The uptake curves reveal complex interactions of adsorption on the outer surface of MOF particles, structural transition, of which the overall rate depends on several factors, including pressure step, temperature, as well as particle size, and the subsequent diffusion into newly opened pores. With the aid of a kinetic model based on the linear driving force (LDF) approach, both rates of diffusion and structural transition were studied independently of each other. It is shown that temperature and applied pressure steps have a strong effect on the rate of structural transition and thus, the overall velocity of gas uptake. For pressure steps close to the upper boundary of the gate-opening, the rate of structural transition is drastically reduced. This feature enables a fine-tuning of the overall velocity of sorption, which can even turn into anti-Arrhenius behavior.
Quasi-2D Mn3Si2Te6 Nanosheet for Ultrafast Photonics
Authors: Lu Yan , Zhou Zheng , Kan Xuefen , Yang Zixin , Deng Haiqin , Liu Bin , Wang Tongtong , Liu Fangqi , Liu Xueyu , Zhu Sicong , Yu Qiang , Wu Jian
Published: February 2023

The magnetic nanomaterial Mn3Si2Te6 is a promising option for spin-dependent electronic and magneto-optoelectronic devices. However, its application in nonlinear optics remains fanciful. Here, we demonstrate a pulsed Er-doped fiber laser (EDFL) based on a novel quasi-2D Mn3Si2Te6 saturable absorber (SA) with low pump power at 1.5 μm. The high-quality Mn3Si2Te6 crystals were synthesized by the self-flux method, and the ultrathin Mn3Si2Te6 nanoflakes were prepared by a simple mechanical exfoliation procedure. To the best of our knowledge, this is the first time laser pulses have been generated using quasi-2D Mn3Si2Te6. A stable pulsed laser at 1562 nm with a low threshold pump power of 60 mW was produced by integrating the Mn3Si2Te6 SA into an EDFL cavity. The maximum power of the output pulse is 783 μW. The repetition rate can vary from 24.16 to 44.44 kHz, with corresponding pulse durations of 5.64 to 3.41 µs. Our results indicate that the quasi-2D Mn3Si2Te6 is a promising material for application in ultrafast photonics.
Association between Cardiovascular Disease and Liver Disease, from a Clinically Pragmatic Perspective as a Cardiologist
Authors: Nakashima Mitsutaka , Nakamura Kazufumi , Nishihara Takahiro , Ichikawa Keishi , Nakayama Rie , Takaya Yoichi , Toh Norihisa , Akagi Satoshi , Miyoshi Toru , Akagi Teiji , Ito Hiroshi
Published: February 2023

Cardiovascular diseases and liver diseases are closely related. Non-alcoholic fatty liver disease has the same risk factors as those for atherosclerotic cardiovascular disease and may also be a risk factor for atherosclerotic cardiovascular disease on its own. Heart failure causes liver fibrosis, and liver fibrosis results in worsened cardiac preload and congestion. Although some previous reports regard the association between cardiovascular diseases and liver disease, the management strategy for liver disease in patients with cardiovascular diseases is not still established. This review summarized the association between cardiovascular diseases and liver disease. In patients with non-alcoholic fatty liver disease, the degree of liver fibrosis progresses with worsening cardiovascular prognosis. In patients with heart failure, liver fibrosis could be a prognostic marker. Liver stiffness assessed with shear wave elastography, the fibrosis-4 index, and non-alcoholic fatty liver disease fibrosis score is associated with both liver fibrosis in patients with liver diseases and worse prognosis in patients with heart failure. With the current population ageing, the importance of management for cardiovascular diseases and liver disease has been increasing. However, whether management and interventions for liver disease improve the prognosis of cardiovascular diseases has not been fully understood. Future investigations are needed.
Association between Ultra-Processed Food Consumption and Metabolic Syndrome among Adults in China—Results from the China Health and Nutrition Survey
Authors: Pan Feng , Wang Zhihong , Wang Huijun , Zhang Jiguo , Su Chang , Jia Xiaofang , Du Wenwen , Jiang Hongru , Li Weiyi , Wang Liusen , Hao Lixin , Zhang Bing , Ding Gangqiang
Published: February 2023

The prevalence of metabolic syndrome (MetS) is increasing and the relationship between ultra-processed food (UPF) consumption and MetS remains uncertain in Chinese adults. This study aimed to examine the longitudinal association of UPF consumption with the risk of MetS and its components in Chinese adults. Adults aged 18 years and above who participated in at least two waves of the China Health and Nutrition Survey (CHNS) in 2009, 2015, and 2018 were included in this analysis. Dietary intake data were collected by three consecutive 24 h dietary recalls and weighing household foods and condiments. Depending on the purpose and extent of food processing, UPFs were classified using the NOVA food classification system. A multivariate Cox proportional risk model was used to explore the association between UPF consumption (grouped by quartile: quartile 1 (Q1), quartile 2 (Q2), quartile 3 (Q3), and quartile 4 (Q4)) and risk of MetS and its components. A total of 5147 adults were included. During a median (IQR) 6.0 (3.0, 9.0) year follow-up with 31,878 person-years, 1712 MetS cases were identified, with an incidence of 33.26%. After multivariable adjustment, the risk of MetS was increased by 17% in the highest quartile with UPF consumption (HR: 1.17, 95% CI: 1.01–1.35, p trend: 0.047), with the lowest quartile as a reference. For the components of MetS, the risk of central obesity, raised triglycerides (TG), reduced high-density lipoprotein cholesterol (HDL-C), and raised blood pressure (BP) was increased by 33% (HR: 1.33, 95% CI: 1.18–1.51, p trend: <0.001), 26% (HR: 1.26, 95% CI: 1.08–1.48, p trend: 0.003), 25% (HR: 1.25, 95% CI: 1.07–1.46, p trend: 0.007), and 16% (HR: 1.16, 95% CI: 1.03–1.32, p trend: 0.018) in the highest quartile with UPF consumption, respectively. Adults aged 45–59 years and living in urban areas with higher UPF consumption had higher odds of MetS. These results indicate that higher long-term UPF consumption was associated with an increased risk of MetS in Chinese adults. Further studies such as intervention trials are needed to confirm the mechanism of correlation between UPF consumption and health-related outcomes. Nutritional education actions are warranted to promote a balanced diet and improve the overall dietary quality of residents to reduce the risk of MetS effectively.
Altered Expression of Antimicrobial Peptides in the Upper Gastrointestinal Tract of Patients with Diabetes Mellitus
Authors: Linn Oliver , Menges Bernhard , Lammert Frank , Weber Susanne N. , Krawczyk Marcin
Published: February 2023

Antimicrobial peptides (AMP) are essential components of innate immunity with a broad range of antimicrobial activities against bacteria, viruses, and fungi. The aim of this study was to investigate AMP expression in the upper gastrointestinal tract in normal and pathological metabolic states in humans. Furthermore, we examined the correlation between vitamin D levels and AMP expression in the same cohort. Serum concentrations of 25-hydroxyvitamin D3 were measured, and mRNA expression of β-defensins HBD-1, -2, -3, -4, α-defensins HD-5 and -6 and cathelicidin in the upper gastrointestinal tract epithelia were determined by quantitative RT-PCR in 31 individuals (10 with type 2 diabetes, 10 with insulin resistance, and 11 healthy controls). The majority of the cohort showed low vitamin D concentrations, which were negatively correlated with mRNA expression levels of HBD-3 in corpus mucosa. HBD-1 and HBD-3 mRNA were expressed in corpus mucosa, with the former significantly decreased in patients with diabetes. Hence, we conclude that type 2 diabetes is associated with reduced AMP expression in the upper gastrointestinal tract, which might contribute towards epithelial barrier dysfunction and increased bacterial translocation in these patients.