bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-contact detection of the interfacial microdefects in metal/CFRP hybrid composites using air-coupled laser ultrasound
Authors: Wang Baoding , Cheng Zhongwen , Liao Weisheng , Long Bainian , Wu Junwei , Zeng Lvming , Ji Xuanrong
Published: March 2024

Metal/CFRP (carbon fiber reinforced plastic) hybrid composites are crucial in aerospace applications, demanding non-contact, high-resolution inspection. Traditional non-destructive testing methods face daunting technical challenges due to varying densities and impedance between fiber and metal layers. Here, a hybrid air-coupled laser-ultrasound (ACLU) system was presented for non-contact detection of internal microdefects on the non-homogeneous metal/CFRP interface. The near-surface resolution of the system can reach as high as 80 µm on a long effective working distance of 50 mm. Three types of experiments, specifically ACLU, phased-array, and X-ray inspection, were performed in Al/CFRP composites with the interfacial microdefects located in Al, bonded, and CFRP layers respectively. Comparing with traditional methods, the proposed ACLU system can detect the microdefects of bonding failure, cohesive failure, and delamination at the Al/CFRP interface with higher contrast, resolution, and sensitivity. A significant advantage of the ACLU system is its ability to detect defects on curved surface structure materials, making it highly versatile for various composite configurations. Our eventual goal is to offer an affordable non-contact laser-ultrasound approach for quality and damage inspection of metal/CFRP hybrid composites.
Enhancing the physical properties of recycled low-density polyethylene and virgin low-density polyethylene blend using octanoate starch
Authors: Al Maamori Mohammed Hamza , Habeeb Salih Abbas , Moslem Mohammed Ali
Published: March 2024

Increasing global consumption of low-density polyethylene leads to more polymeric waste and challenges solid waste management. Therefore, the process of recycling or mixing recycled low-density polyethylene (RLDPE) with the same virgin low-density polyethylene (VLDPE) and enhancing the properties of this blend by adding different amounts of bioplastic such as Octanoate starch (OCST) is a good contribution to getting rid of plastic waste and obtaining new materials. This paper mixed the 20, 30 and 50 wt.% from RLDPE with VLDPE to determine the homogeneous blend. Also, 30, 40, and 50 wt. % from OCST were blended with homogeneous as a 20:80 RLDPE: VLDPE to obtain a new hybrid blend containing bioplastic. The scanning electron microscope (SEM) results showed the high surface roughness of the film RLDPE: VLDPE after adding the OCST. The tensile strength, elastic modulus, and hardness of the 20:80 RLDPE: VLDPE blend were 13.0 ± 1.467 MPa, 0.08 ± 0.016 MPa, and 42.3 ± 2.32 Shore A, while the tensile strength, elastic modulus, and hardness of the 12:48:40 RLDPE: VLDPE: OCST were 14.3 ± 1.626 MPa, 0.1 ± 0.032 MPa, and 50.1 ± 2.78 Shore A respectively. On the other hand, the Fourier-Transform Infrared (FTIR) indicated that the strong bands at 1746 cm−1, 2856 cm−1, and 2927 cm−1 indicated stretching deformation of the ester carbonyl group and the methyl and methylene groups of OCST with blend polymers, respectively. The thermal properties as melting point, heat energy consumption, and heat flow difference increased from 122.52 to 123.73°C, −389.41 to −144.43 (mJ), and −77.88 to −28.89 (J/g) when the OCST content increased from 20:80 RLDPE: VLDPE to 12:48:40 RLDPE: VLDPE: OCST. Thermal decomposition led to the formation of functional groups such as hydroxyl and carbonyl on LDPE baskets and an increase in the carbonyl index after adding OCST to the RLDPE: VLDPE blend. The best hybrid blend was 12:48:40 RLDPE: VLDPE: OCST.
When Anti-CRT Becomes Anti-Care: Navigating Curricular Controversies Amid Voucher-Induced Changes to a Private Religious School Landscape
Authors: Giunco Kierstin , Sellers Kathleen M.
Published: March 2024

As access to private religious education expands through vouchers, public discourse has positioned these schools as politically neutral spaces. Teachers who seek to ethically care for students are thus placed in a predicament. In this article, we present the fictive case study of a middle school teacher in a suburban Catholic school that has accepted vouchers and consequently undergone significant demographic and political shifts. When the teacher makes a curricular decision that responds in caring and critical ways to their students, they face a wave of parental opinions that call their instruction and ethical aims into question.
Intramedullary Screw Fixation of Proximal Phalangeal Fractures: Short- to Medium-term Outcomes
Authors: Abbot Hagen , George Adam R. , McCarron Luke , Graham David J. , Sivakumar Brahman
Published: March 2024

Background:

Intramedullary screw fixation of phalangeal and metacarpal fractures has gained popularity as a method of providing rigid internal fixation with minimum tendon disturbance, allowing early mobilization and rehabilitation. Despite this, the literature on outcomes using this technique is limited. Thus, the aim of this study was to assess the short-term to medium-term outcomes of intramedullary screw fixation for proximal phalangeal fracture fixation in an Australian setting.

Methods:

A retrospective analysis of consecutive patients with a fracture(s) of the proximal phalanx fixated using intramedullary screws between January 2020 and March 2023 was conducted via telehealth.

Results:

Forty-six phalangeal fractures from 37 patients were included in this study. Mean Quick Disabilities of the Arm, Shoulder, and Hand score of 6.2 (range: 0-61.4, median: 0), mean pain visual analogue scale score of 1.8 (range: 1-7, median: 1), and mean total active motion of 231° (range: 132-282) were noted. A total of 4.4% of fractures sustained a major complication, and 94.6% of patients reported willingness to undergo intramedullary screw fixation again.

Conclusion:

This study concurs with the prior literature that intramedullary screw fixation is a safe and effective method of stabilizing extra-articular proximal phalangeal fractures in the short to medium term. Superior clinical outcomes, as well as cost-effectiveness and time-effectiveness, render it a viable alternative to plate fixation in this setting.
Triceps Tendon Reattachment Using Mini Plates and Screws After Failure of Olecranon Avulsion Fracture Fixation in Osteoporotic Bone: A Case Report
Authors: Pohl Nicholas B. , Brush Parker L. , Russo James , Fras Sebastian I. , Beredjiklian Pedro K. , Fletcher Daniel J.
Published: March 2024

This is a case report of an 85-year-old woman with osteopenia who underwent olecranon avulsion fracture repair with supplemental triceps tendon repair following a fall on an outstretched arm. The initial procedure failed due to osteoporotic bone quality and an atraumatic disruption of the olecranon fracture fixation. The patient subsequently underwent further surgical intervention with an olecranon avulsion fracture excision and a novel triceps tendon repair technique using plate augmentation and fiber tape. Surgeons may consider this novel approach as an initial treatment for elderly patients with osteopenia or osteoporosis undergoing olecranon avulsion fracture fixation, to prevent the failure and consequent revision surgery.
Diagnosis and treatment of venous thromboembolism during pregnancy relate to genetic polymorphism
Authors: Yang Qingcheng , Wang Xuechang , Wang Rui , Li Aihua
Published: March 2024

Objectives

Previous research had shown that age, a positive family history, comorbidities, major surgical operations, gestation, and use of several medications could increase the incidence of venous thromboembolism (VTE). With the development of medical and clinical individualized treatment, many people exposed to above risk factors did not develop VTE, suggested that genetic factors are also involved in the development of VTE. In this review, we aim to summarize VTE diagnosis and treatment in pregnancy women related to gene polymorphism.

Methods

A comprehensive electronic search using PubMed, MEDLINE, EMBASE and Web of Science was conducted to find relevant journal articles with key search terms including: “pregnancy OR pregnant,” “venous thromboembolism OR VTE,” “deep vein thrombosis OR DVT,” “pulmonary embolism OR PE,” and “genetic OR gene.” Prominent publications from establishment of database till present were analysed to achieve a deeper understanding of VTE during pregnancy relate to genetic polymorphism, and the information was then collated to form this review.

Results

The literature review revealed that inherited thrombophilia significantly associated with the development of VTE, especially the factor V Leiden (FVL) and prothrombin gene mutation (PGM). Furthermore, the role of methylenetetrahydrofolate reductase (MTHFR) gene mutation in the development of pregnancy-related VTE remains controversial, further study is required. In the present study, Marburg I polymorphism (G511 E), c.1538 G>A and c.1601 G>A in Factor V (FV), JAK2V617 F mutation were reported as an independent risk factor for VTE, there is no sufficient evidence to confirm the gene mutation is related to VTE during pregnancy, these factors appearing as another promising potential diagnostic marker of VTE during pregnancy. Besides, the dosages of heparin in the treatment of VTE during pregnancy need be adjusted according to gene polymorphism of these population, particularly FVL or PGM carriers, and this area is not studied deeply, it is worth further study.

Conclusion

Inherited thrombophilia significantly associated with the development of VTE, especially the FVL and PGM, however the relation between MTHFR gene mutation and pregnancy-related VTE remains controversial, further study is needed. In addition, the dosages of heparin in the treatment of VTE during pregnancy suggested to adjusted based on gene polymorphism in FVL and PGM, and establish better prediction models is a direction of future research.
From research misconduct to disciplinary sanction: an empirical examination of French higher education case law
Authors: Leclerc Olivier , Klausser Nicolas
Published: March 2024

Reporting and investigating research misconduct can lead to disciplinary proceedings being initiated, and ultimately to disciplinary sanctions being imposed on convicted scientists. The conversion of research misconduct findings into disciplinary sanctions is poorly understood. This article analyses all the disciplinary decisions handed down on appeal by the Conseil national de l'enseignement supérieur et de la recherche (CNESER) between 1991 and 2023, concerning breaches of research integrity by academics and doctoral students ( n = 333). Three findings are highlighted. Firstly, the article describes how the CNESER sanctioned research misconduct even before the notion of research integrity became part of French law, by monitoring scientists’ compliance with “deontological rules”. Secondly, we show that assessing disciplinary fault involves evaluating a much broader set of circumstances than the mere existence of research misconduct, which can explain why the latter do not result in disciplinary sanctions or lighter sanctions. Thirdly, the research highlights situations where research misconduct is intertwined with other allegations, blurring the relative importance of these motives in the awarding of disciplinary sanctions. The article concludes with a call for greater accessibility to the disciplinary decisions handed down by universities in the first instance, as a key next step in gaining a better understanding of the disciplinary response to research misconduct.
Revisiting the influence of phonological similarity on cognate processing: Evidence from Cantonese–Japanese bilinguals
Authors: Wong Brian W.L. , Hemelstrand Shawn , Inoue Tomohiro
Published: March 2024

The influences of shared orthography, semantics, and phonology on bilingual cognate processing have been investigated extensively. However, mixed results have been found regarding the effects of phonological similarity on L2 cognate processing. In addition, most existing studies examining the influence of phonological similarity on cognate processing have been conducted on alphabetic scripts, in which phonology and orthography are always associated. Hence, in this study, we recruited Cantonese–Japanese bilinguals who used two logographic scripts, traditional Chinese and Japanese Kanji, to examine the influence of phonological similarity on L2 cognate lexical decision. Importantly, these scripts allow the manipulation of phonological similarity using identical characters across both languages. In addition, we examined how word frequency and L2 proficiency modulate cognate processing. Results showed that although word frequency and L2 proficiency played important roles in cognate processing, there was minimal overall influence of phonological similarity on cognate lexical decision. The latter finding suggests that theoretical models of bilingual word recognition may need to be refined to enhance our understanding of cognate processing regarding the role of phonology among diverse bilingual populations.
Reading ‘between the lines’: How implicit language helps liberal media survive in authoritarian regimes. The Kommersant Telegram posts case study
Authors: Tymbay Alexey
Published: March 2024

This case study demonstrates identification, explicitation, and validation of the implicatures found in the Kommersant (Russia) Telegram channel posts. It explores the primary reasons for Kommersant’s implicature use and the language means employed for the creation of the implicature. The contributors to the Kommersant Telegram channel use irony/sarcasm, creative neologisms, wordplay, metaphors, and legally imposed euphemisms for the expression of the implicit meaning. The use of implicatures is mainly motivated by the authors’ desire for self-protection and cooperation. Kommersant’s implicit language also contributes towards the creation of a circle of loyal readers who may enjoy explicitating the implicatures so as to feel they belong to a specific socio-political group. The multi-stage perceptual analysis substantiates the assumption that readers with prior knowledge of Kommersant’s style are more sensitive to its implicatures. The report also concludes that at times of rigid war-time media regulation in Russia, Kommersant’s reporters use a code of implicit expressions as a means to preserve a certain objectivity in their reporting and to maintain their loyal readership.
Hepatitis B and hepatitis C testing practices and observed seroconversions among dialysis facilities in Georgia
Authors: Butsashvili Maia , Kanchelashvili Giorgi , Aslanikashvili Ana , Kuchuloria Tinatin , Shadaker Shaun , Tskhomelidze Irina , Tsereteli Maia , Kamkamidze George , Handanagic Senad , Patel Priti R , Armstrong Paige A
Published: March 2024

Background

Individuals receiving hemodialysis for end-stage kidney disease are at increased risk of infection with hepatitis C virus (HCV) and hepatitis B virus (HBV) due to regular and frequent receipt of invasive medical treatment in a shared space.

Aim

This study assessed infection prevention and control practices in dialysis facilities, evaluated HCV and HBV testing practices, and estimated the number of cases of seroconversion for HCV and HBV infection in dialysis facilities in Georgia.

Methods

We invited all 27 dialysis centers that provide maintenance dialysis in Georgia to participate in a facility-based survey during April–June 2021.

Results

In total, 68.2% ( n = 15/22) of facilities performed anti-HCV screening upon admission to the center. At the majority of facilities ( n = 21/22, 95.5%), HBV screening was performed upon admission to the center. A total of 329 anti-HCV positive patients were reported from 20 of 22 facilities, 29.5% ( n = 97/329) were HCV RNA positive, 18.2% ( n = 60/329) were HCV RNA negative, and 52.3% ( n = 172/329) were not tested or their result was missing. Overall, 200 HBsAg-positive patients were reported from the same 20 facilities. At 10 facilities: 39 patients from seven facilities seroconverted for HCV infection, and 31 patients from eight dialysis facilities seroconverted for HBV infection.

Conclusions

We identified a high number of HBV and HCV seroconversions among dialysis patients in Georgia suggesting serious gaps in infection control practices. Strict adherence to infection prevention and control practices is essential to prevent transmission of HCV and HBV infections through contaminated equipment and surfaces.
A comparison of screw and suture button fixation in the management of adolescent ankle syndesmotic injuries
Authors: Verlinsky Luke , Heath David M , Momtaz David A , Christopher Boris , Singh Aaron , Gibbons Steven D
Published: March 2024

Purpose:

Ankle injuries involving the tibiofibular syndesmosis often necessitate operative fixation to restore stability to the ankle. Recent literature in the adult population has suggested that suture button fixation may be superior to screw fixation. There is little evidence as to which construct is preferable in the pediatric and adolescent population. This study investigates outcomes of suture button and screw fixation in adolescent ankle syndesmotic injuries.

Methods:

A retrospective matched cohort study over 10 years of pediatric patients who underwent ankle syndesmotic fixation at a large Level 1 Trauma Center was conducted. Both isolated syndesmotic injuries and ankle fractures with syndesmotic disruption were included. Preoperative variables collected include basic patient demographics, body mass index, and fracture type. Suture button and screw cohorts were matched based on age, race, sex, and open fracture utilizing propensity scores. Outcomes assessed include reoperation and implant failure.

Results:

A total of 44 cases of operative fixation of the ankle syndesmosis were identified with a mean age of 16 years. After matching cohorts based on age, sex, race, and open fracture status, there were 17 patients in the suture button and screw cohorts, respectively. Patients undergoing screw fixation had a six times greater risk of reoperation (p = 0.043) and 13 times greater risk of implant failure (p < 0.001). Out of six cases of reoperation in the screw cohort, five were unplanned.

Conclusion:

Our findings favor suture button fixation in operative management of adolescent tibiofibular syndesmotic injuries. Compared with screws, suture buttons are associated with lower risk of both reoperation and implant failure.

Level of evidence:

level III therapeutic.
Aneurysm of the Artery of Wollschlaeger and Wollschlaeger
Authors: Alkiswani Abdul-Rahman , Heskett Cody , Fry Lane , Gausper Adeesya , Le Kevin , Brake Aaron , Lei Catherine , De Stefano Frank A , Madarang Ernest J , Peterson Jeremy , Ebersole Koji
Published: March 2024

The Artery of Wollschlaeger and Wollschlaeger (AWW) is a non-eloquent, tentorial branch of the superior cerebellar artery (SCA). Coursing posteriorly from an intradural origin, the AWW passes through the ambient cistern and supplies the medial tentorium. Due to its small diameter, the AWW is often only identified in the context of secondary dilation from pathologies such as dural arteriovenous fistulas (DAVF). Herein, we report the first case, to our knowledge, of an aneurysm of the AWW associated with a posterior fossa DAVF Swift identification and diagnosis followed by cautious treatment of both the aneurysm and fistula were critical to avert rupture and optimize outcomes, avoiding potential hemorrhagic complications.
Identifying students’ perceptions, preferences and challenges of online learning during COVID-19 pandemic in Aligarh Muslim University, India
Authors: Munshi Shamim Aktar , Barsha Sayantoni
Published: March 2024

Due to the rapid spread of the COVID-19, educational institutes around the world, including India, were forced to close their doors and transition to an online mode of education. The purpose of this study was to analyse the perception and preference towards online learning among students of Aligarh Muslim University in India during this pandemic. Additionally, this study examines the level of awareness regarding various online learning platforms introduced by the University Grants Commission (UGC). A quantitative survey method was applied to achieve the goal of this study. An online questionnaire (Google form) was circulated among students via WhatsApp groups. A total of 234 students responded from different departments, analysis of the data was done with the help of SPSS. The findings of the study revealed that more than 90 percent of respondents preferred online learning to manage their course curriculum during the pandemic, as there were no other alternatives available. The study found that most of the respondents were familiar with various online learning platforms introduced by the UGC. However, a significant number of respondents reported issues with online learning, such as technological constraints, an inappropriate learning environment, various health issues, one-way communication, etc. These findings may be useful in constructing education policies and plans for online learning to make it more effective for students.
Advancing Community Care and Access to Follow-up After Acute Kidney Injury Hospitalization: Design of the AFTER AKI Randomized Controlled Trial
Authors: Bhatt Meha , Benterud Eleanor , Palechuk Taylor , Bignell Coralea , Ahmed Nasreen , McBrien Kerry , James Matthew T. , Pannu Neesh
Published: March 2024

Background:

Acute kidney injury (AKI) is a common complication among hospitalized patients with long-term implications including chronic kidney disease (CKD). Although models are available to predict the risk of advanced CKD after AKI, there is limited evidence regarding follow-up for patients with AKI after hospital discharge, resulting in variable follow-up care. A risk-stratified follow-up approach may improve appropriateness and efficiency of management for CKD among patients at risk of declining kidney function following AKI.

Objective:

The objective was to compare and evaluate the use of a risk-stratified approach to follow-up care vs usual care for patients with AKI after hospital discharge.

Design:

This study was a pragmatic randomized controlled trial.

Setting:

This study was conducted in 2 large urban hospitals in Alberta, Canada.

Patients:

Hospitalized patients with AKI (KDIGO stage 2 or 3) not previously under the care of a nephrologist, expected to survive greater than 90 days being discharged home.

Measurements:

We will evaluate whether guideline-recommended CKD care processes are initiated within 90 days, including statin use, angiotensin-converting enzyme inhibitor (ACEi)/angiotensin II receptor blocker (ARB) use in those with proteinuria or diabetes, and nephrologist follow-up if sustained eGFR <30 mL/min/1.73 m2. We will also assess the feasibility of recruitment and the proportion of patients completing the recommended blood and urine tests at 90 days.

Methods:

Patients with AKI will be enrolled and randomized near the time of hospital discharge. In the intervention group, low risk patients will receive information regarding AKI, medium risk patients will additionally receive follow-up guidance sent to their primary care physician, and high-risk patients will additionally receive follow-up with a nephrologist. Participants in the intervention and usual care group will receive a requisition for urine testing and bloodwork at 90 days following hospital discharge. Telephone follow-up will be conducted for all study participants at 90 days and 1 year after hospital discharge. Bivariate tests of association will be conducted to evaluate group differences at the follow-up time points.

Limitations:

We expect there may be challenges with recruitment due to the significant co-existence of comorbidity in this population.

Conclusions:

If the trial shows a positive effect on these processes for kidney care, it will inform larger-scale trial to determine whether this intervention reduces the incidence of long-term clinical adverse events, including CKD progression, cardiovascular events, and mortality following hospitalization with AKI.
Characteristics of Homeless Temporarily-Housed in Project RoomKey During the COVID-19 Pandemic
Authors: Sloan Kylie , Kogan Alexis Coulourides , Guller Jodie , Feldman Corinne T. , Feldman Brett J.
Published: March 2024

Introduction:

People experiencing unsheltered homelessness (PEUH) have higher disease burden yet limited access to healthcare. COVID-19 introduced even greater risk for PEUH aged 65+ years with an underlying chronic health condition and were temporarily housed in hotels/motels for Project RoomKey (PRK). This study aimed to characterize a PRK cohort who received primary care from a street medicine program.

Methods:

This observational case series study included a sample of 35 PRK participants receiving primary care from a street medicine team at a single site from July to September 2020. We used the HOUSED BEDS assessment tool for taking history on PEUH.

Results:

Participants were 63% male, 40% Hispanic/Latino/a, 40% white, 94% English-speaking, and 73% had chronic health conditions. Assessment revealed: average Homelessness (H) of 4 years; 76% had no prior social service Outreach (O); average Utilization (U) was 4 emergency department visits in prior 6-months; 68% received Salary (S) from government income; Food access or Eat (E) was commonly purchased (29%) or donated (26%); clean water to Drink (D) for 59% of participants; 86% had access to a Bathroom (B); Encampment (E) was varied and 38% reported safety concerns; Daily routine (D) showed 76% could access a telephone, 32% received social support from family; 79% reported past or current Substance use (S). No participants contracted COVID-19 during study period.

Conclusions:

This study describes health and demographic characteristics of PRK participants in Southern California. Findings inform policies to continue PRK that includes onsite healthcare such as via street medicine.
Show HBCU Referee Bias the Red Card: Testing Communication Theory in Division I Women’s College Soccer
Authors: Dix Andrew
Published: March 2024

The current study focused on the red cards and yellow cards that referees gave to historically Black colleges and universities (HBCUs) in Division I women’s college soccer for their in-game communicative actions. These cards are distributed to players who are perceived to have engaged in a reckless play or an action that involved excessive force. Within the United States, HBCUs are institutions of higher education that historically and primarily serve students who are of African American descent. Thus, the strong Black woman collective (SBWC) theory of communication was the theoretical frame because this study focused on small groups that were mostly comprised of African American women. Previous studies on referee bias were summarized in the literature review. Publicly available data on 19,360 soccer games were then analyzed. The findings indicated that referees gave more red cards to HBCU women’s college soccer teams relative to predominantly White institution (PWI) women’s college soccer teams at a statistically significant level. Yellow cards were also disproportionately distributed to HBCUs relative to PWIs. Implications for communication theory were noted in the study discussion as were interpretations on why referee bias against HBCUs continues to occur.
Evaluation of fatigue life of fiberglass reinforced polyester composite materials using Weibull analysis methods
Authors: Djeghader Djamel , Redjel Bachir
Published: March 2024

A composite material based on a polyester resin and fiberglass reinforcement was fabricated by the contact molding process with a fiber percentage of 40%. This material underwent a cyclic fatigue loading and 3-point bending at slow speed. The experimental results were used to plot the S–N curve based on a linear function, Hwang & Han’s relation and Basquin’s hypothesis. A probabilistic analysis based on the Weibull distribution with two and three parameters was used in order to adjust and predict the fatigue behavior of the material used in the study. This analysis made it possible to plot the fatigue curve for different levels of reliability (Ps = 99%, Ps = 90%, Ps = 50%, Ps = 36.8% and Ps = 10%), in order to identify first time to failure for material reliability and safety limits.
Integration of scRNA-seq data by disentangled representation learning with condition domain adaptation
Authors: Liu Renjing , Qian Kun , He Xinwei , Li Hongwei
Published: March 2024

Background

The integration of single-cell RNA sequencing data from multiple experimental batches and diverse biological conditions holds significant importance in the study of cellular heterogeneity.

Results

To expedite the exploration of systematic disparities under various biological contexts, we propose a scRNA-seq integration method called scDisco, which involves a domain-adaptive decoupling representation learning strategy for the integration of dissimilar single-cell RNA data. It constructs a condition-specific domain-adaptive network founded on variational autoencoders. scDisco not only effectively reduces batch effects but also successfully disentangles biological effects and condition-specific effects, and further augmenting condition-specific representations through the utilization of condition-specific Domain-Specific Batch Normalization layers. This enhancement enables the identification of genes specific to particular conditions. The effectiveness and robustness of scDisco as an integration method were analyzed using both simulated and real datasets, and the results demonstrate that scDisco can yield high-quality visualizations and quantitative outcomes. Furthermore, scDisco has been validated using real datasets, affirming its proficiency in cell clustering quality, retaining batch-specific cell types and identifying condition-specific genes.

Conclusion

scDisco is an effective integration method based on variational autoencoders, which improves analytical tasks of reducing batch effects, cell clustering, retaining batch-specific cell types and identifying condition-specific genes.
Using protein language models for protein interaction hot spot prediction with limited data
Authors: Sargsyan Karen , Lim Carmay
Published: March 2024

Background

Protein language models, inspired by the success of large language models in deciphering human language, have emerged as powerful tools for unraveling the intricate code of life inscribed within protein sequences. They have gained significant attention for their promising applications across various areas, including the sequence-based prediction of secondary and tertiary protein structure, the discovery of new functional protein sequences/folds, and the assessment of mutational impact on protein fitness. However, their utility in learning to predict protein residue properties based on scant datasets, such as protein–protein interaction (PPI)-hotspots whose mutations significantly impair PPIs, remained unclear. Here, we explore the feasibility of using protein language-learned representations as features for machine learning to predict PPI-hotspots using a dataset containing 414 experimentally confirmed PPI-hotspots and 504 PPI-nonhot spots.

Results

Our findings showcase the capacity of unsupervised learning with protein language models in capturing critical functional attributes of protein residues derived from the evolutionary information encoded within amino acid sequences. We show that methods relying on protein language models can compete with methods employing sequence and structure-based features to predict PPI-hotspots from the free protein structure. We observed an optimal number of features for model precision, suggesting a balance between information and overfitting.

Conclusions

This study underscores the potential of transformer-based protein language models to extract critical knowledge from sparse datasets, exemplified here by the challenging realm of predicting PPI-hotspots. These models offer a cost-effective and time-efficient alternative to traditional experimental methods for predicting certain residue properties. However, the challenge of explaining why specific features are important for determining certain residue properties remains.
A new sphenodontian (Diapsida: Lepidosauria) from the Upper Triassic (Norian) of Germany and its implications for the mode of sphenodontian evolution
Authors: Freisem Lisa S. , Müller Johannes , Sues Hans-Dieter , Sobral Gabriela
Published: March 2024

The Arnstadt Formation of Saxony-Anhalt, Germany has yielded some of Germany’s most substantial finds of Late Triassic tetrapods, including the sauropodomorph Plateosaurus and the stem-turtle Proganochelys quenstedti. Here, we describe an almost complete skull of a new sphenodontian taxon from this formation (Norian, 227–208 Ma), making it the oldest known articulated sphenodontian skull from Europe and one of the oldest in the world. The material is represented by the dermal skull roof and by the complete maxilla and temporal region, as well as parts of the palate, braincase, and lower jaw. A phylogenetic assessment recovers it as a basal sphenodontian closely related to Planocephalosaurus robinsonae and to Eusphenodontia, making it the earliest-diverging sphenodontian known with an articulated skull. Its cranial anatomy is generally similar to the well-known Diphydontosaurus avonis from the Rhaetian of England, showing that this successful phenotype was already established in the clade around 10 myr earlier than assumed. An analysis of evolutionary change rates recovers high rates of evolution in basal sphenodontians, with decreasing rates throughout the evolution of the group. However, contrary to previous studies, reversals in this trend were identified, indicating additional peaks of evolutionary change. These results improve our understanding of the early sphenodontian diversity in Europe, providing critical information on evolutionary trends throughout the history of the clade and sparking renewed interest in its evolution.
Serum electrolyte concentrations and risk of atrial fibrillation: an observational and mendelian randomization study
Authors: Wu Yang , Kong Xiang-Jun , Ji Ying-Ying , Fan Jun , Ji Cheng-Cheng , Chen Xu-Miao , Ma Yue-Dong , Tang An-Li , Cheng Yun-Jiu , Wu Su-Hua
Published: March 2024

Background

Atrial fibrillation (AF) is a prevalent arrhythmic condition resulting in increased stroke risk and is associated with high mortality. Electrolyte imbalance can increase the risk of AF, where the relationship between AF and serum electrolytes remains unclear.

Methods

A total of 15,792 individuals were included in the observational study, with incident AF ascertainment in the Atherosclerosis Risk in Communities (ARIC) study. The Cox regression models were applied to calculate the hazard ratio (HR) and 95% confidence interval (CI) for AF based on different serum electrolyte levels. Mendelian randomization (MR) analyses were performed to examine the causal association.

Results

In observational study, after a median 19.7 years of follow-up, a total of 2551 developed AF. After full adjustment, participants with serum potassium below the 5th percentile had a higher risk of AF relative to participants in the middle quintile. Serum magnesium was also inversely associated with the risk of AF. An increased incidence of AF was identified in individuals with higher serum phosphate percentiles. Serum calcium levels were not related to AF risk. Moreover, MR analysis indicated that genetically predicted serum electrolyte levels were not causally associated with AF risk. The odds ratio for AF were 0.999 for potassium, 1.044 for magnesium, 0.728 for phosphate, and 0.979 for calcium, respectively.

Conclusions

Serum electrolyte disorders such as hypokalemia, hypomagnesemia and hyperphosphatemia were associated with an increased risk of AF and may also serve to be prognostic factors. However, the present study did not support serum electrolytes as causal mediators for AF development.
Molecular adaptations underlying high-frequency hearing in the brain of CF bats species
Authors: Li Xintong , Wang Hui , Wang Xue , Bao Mingyue , Sun Ruyi , Dai Wentao , Sun Keping , Feng Jiang
Published: March 2024

Background

The majority of bat species have developed remarkable echolocation ability, especially for the laryngeally echolocating bats along with high-frequency hearing. Adaptive evolution has been widely detected for the cochleae in the laryngeally echolocating bats, however, limited understanding for the brain which is the central to echolocation signal processing in the auditory perception system, the laryngeally echolocating bats brain may also undergo adaptive changes.

Result

In order to uncover the molecular adaptations related with high-frequency hearing in the brain of laryngeally echolocating bats, the genes expressed in the brain ofRhinolophus ferrumequinum(CF bat) andMyotis pilosus(FM bat) were both detected and also compared. A total of 346,891 genes were detected and the signal transduction mechanisms were annotated by the most abundant genes, followed by the transcription. In hence, there were 3,088 DEGs were found between the two bat brains, with 1,426 highly expressed in the brain ofR. ferrumequinum, which were significantly enriched in the neuron and neurodevelopmental processes. Moreover, we found a key candidate hearing gene,ADCY1, playing an important role in theR. ferrumequinumbrain and undergoing adaptive evolution in CF bats.

Conclusions

Our study provides a new insight to the molecular bases of high-frequency hearing in two laryngeally echolocating bats brain and revealed different nervous system activities during auditory perception in the brain of CF bats.
High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China
Authors: Zhan Qing , Teng Gaoqin , Chen Weiwei , Yu Xiao
Published: March 2024

Objectives

Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging global health threat, resulting in significant morbidity and mortality worldwide. This study aims to determine the molecular characteristics and antimicrobial susceptibility of 263 MRSA isolates in Zhejiang Province, east China.

Methods

From 2014 to 2019, a total of 263 MRSA isolates from bloodstream infections (BSIs) were collected from 6 hospitals in 4 cities in Zhejiang province, east China. Antimicrobial susceptibility tests were conducted according to the guidelines set forth by the Clinical and Laboratory Standards Institute (CLSI). To characterize and analyze these isolates, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) typing and virulence genes gene profiles were performed.

Results

The most predominant clone was ST5-SCCmec II-t311, which accounted for 41.8% (110/263), followed by ST59 (44/263, 16.7%). Compared with non-ST5-II-t311 isolates, ST5-II-t311 isolates were more resistant to erythromycin, tetracycline, levofloxacin, moxifloxacin, and ciprofloxacin, but more susceptible to clindamycin. Moreover, the rates of multidrug resistance were higher in ST5-II-t311 isolates compared to the non-ST5-II-t311 isolates. In comparison to the non-ST5-II-t311 isolates, ST5-II-t311 isolates showed no significant difference in virulence genes detected.

Conclusions

MRSA ST5-II-t311 clone has become the most predominant clone in Zhejiang Province, east China and has higher rates of multidrug resistance than other isolates, that should be kept in mind when treating BSI. Moreover, MRSA ST59 clone shows an upward trend and has begun to spread into hospitals. Our findings highlight the importance of epidemiological studies of S. aureus carriage in the eastern region.
High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice
Authors: Li Fengjiao , Yang Shuo , Ma Ji , Zhao Xiaowen , Chen Meng , Wang Ye
Published: March 2024

Background

To investigate the differences in bacterial and fungal community structure and diversity in conjunctival tissue of healthy and diabetic mice.

Methods

RNA-seq assays and high-throughput sequencing of bacterial 16 S rDNA and fungal internal transcribed spacer (ITS) gene sequences were used to identify differentially expressed host genes and fungal composition profiles in conjunctival tissues of diabetic BKS-db/db mice and BKS (control) mice. Functional enrichment analysis of differentially expressed genes and the correlation between the relative abundance of bacterial and fungal taxa in the intestinal mucosa were also performed.

Results

Totally, 449 differential up-regulated genes and 1,006 down-regulated genes were identified in the conjunctival tissues of diabetic mice. The differentially expressed genes were mainly enriched in metabolism-related functions and pathways. A decrease in conjunctival bacterial species diversity and abundance in diabetic mice compared to control mice. In contrast, fungal species richness and diversity were not affected by diabetes. The microbial colonies were mainly associated with cellular process pathways regulating carbohydrate and lipid metabolism, as well as cell growth and death. Additionally, some interactions between bacteria and fungi at different taxonomic levels were also observed.

Conclusion

The present study revealed significant differences in the abundance and composition of bacterial and fungal communities in the conjunctival tissue of diabetic mice compared to control mice. The study also highlighted interactions between bacteria and fungi at different taxonomic levels. These findings may have implications for the diagnosis and treatment of diabetes.
Genome-wide systematic survey and analysis of the RNA helicase gene family and their response to abiotic stress in sweetpotato
Authors: Mu Fangfang , Zheng Hao , Zhao Qiaorui , Zhu Mingku , Dong Tingting , Kai Lei , Li Zongyun
Published: March 2024

Sweetpotato (Ipomoea batatas (L.) Lam.) holds a crucial position as one of the staple foods globally, however, its yields are frequently impacted by environmental stresses. In the realm of plant evolution and the response to abiotic stress, the RNA helicase family assumes a significant role. Despite this importance, a comprehensive understanding of the RNA helicase gene family in sweetpotato has been lacking. Therefore, we conducted a comprehensive genome-wide analysis of the sweetpotato RNA helicase family, encompassing aspects such as chromosome distribution, promoter elements, and motif compositions. This study aims to shed light on the intricate mechanisms underlying the stress responses and evolutionary adaptations in sweetpotato, thereby facilitating the development of strategies for enhancing its resilience and productivity. 300 RNA helicase genes were identified in sweetpotato and categorized into three subfamilies, namely IbDEAD, IbDEAH and IbDExDH. The collinearity relationship between the sweetpotato RNA helicase gene and 8 related homologous genes from other species was explored, providing a reliable foundation for further study of the sweetpotato RNA helicase gene family's evolution. Furthermore, through RNA-Seq analysis and qRT-PCR verification, it was observed that the expression of eight RNA helicase genes exhibited significant responsiveness to four abiotic stresses (cold, drought, heat, and salt) across various tissues of ten different sweetpotato varieties. Sweetpotato transgenic lines overexpressing the RNA helicase gene IbDExDH96 were generated using A.rhizogenes-mediated technology. This approach allowed for the preliminary investigation of the role of sweetpotato RNA helicase genes in the response to cold stress. Notably, the promoters of RNA helicase genes contained numerous cis-acting elements associated with temperature, hormone, and light response, highlighting their crucial role in sweetpotato abiotic stress response.
Effect of deep neuromuscular block on the quality of early recovery after sleeve gastrectomy in obese patients: a randomized controlled trial
Authors: Yang Wan-li , Wen Ya-ling , Xu Wen-mei , Xu Chi-liang , Yin Wen-qin , Lin Jing-yan
Published: March 2024

Background

Deep neuromuscular block (NMB) has been shown to improve surgical conditions and alleviate post-operative pain in bariatric surgery compared with moderate NMB. We hypothesized that deep NMB could also improve the quality of early recovery after laparoscopic sleeve gastrectomy (LSG).

Methods

Eighty patients were randomized to receive either deep (post-tetanic count 1–3) or moderate (train-of-four count 1–3) NMB. The QoR-15 questionnaire was used to evaluate the quality of early recovery at 1 day before surgery (T0), 24 and 48 h after surgery (T2, T3). Additionally, we recorded diaphragm excursion (DE), postoperative pain, surgical condition, cumulative dose of analgesics, time of first flatus and ambulation, post-operative nausea and vomiting, time of tracheal tube removal and hospitalization time.

Main results

The quality of recovery was significantly better 24 h after surgery in patients who received a deep versus moderate block (114.4 ± 12.9 versus 102.1 ± 18.1). Diaphragm excursion was significantly greater in the deep NMB group when patients performed maximal inspiration at T2 and T3 (P < 0.05). Patients who underwent deep NMB reported lower visceral pain scores 40 min after surgery; additionally, these patients experienced lower pain during movement at T3 (P < 0.05). Optimal surgical conditions were rated in 87.5% and 64.6% of all measurements during deep and moderate NMB respectively (P < 0.001). The time to tracheal tube removal was significantly longer in the deep NMB group (P = 0.001). There were no differences in other outcomes.

Conclusion

In obese patients receiving deep NMB during LSG, we observed improved QoR-15 scores, greater diaphragmatic excursions, improved surgical conditions, and visceral pain scores were lower. More evidence is needed to determine the effects of deep NMB on these outcomes.

Trial registration

ChiCTR2200065919. Date of retrospectively registered: 18/11/2022.