bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Degree of Milling on the Nutraceutical Content in Ecofriendly and Conventional Rice (Oryza sativa L.)
Authors: Kim Seung-Hyun , Yang Yu-Jin , Chung Ill-Min
Published: September 2020

We investigated the effects of the type of rice and degree of milling (DOM) on the nutraceutical content and antioxidant activity of rice (Oryza sativa L.). The fatty acid (FA), vitamin E homolog, and phenolic contents in organic (OR), pesticide-free (PFR), and conventional rice (CR) decreased significantly with an increase in the DOM of rice grains, particularly for a DOM of 7 and 9 (p < 0.05). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity also decreased with the DOM; particularly, this activity decreased significantly, by approximately 60%, in rice grains with a DOM between 7 and 11, as compared to that of brown rice (p < 0.05). α-Tocopherol (r = 0.854) and p-coumaric acid (r = 0.501) showed the strongest correlation with DPPH activity in each chemical group. Stepwise discriminant analysis enabled the correct original and cross-validated classification of 87.0% and 81.5% of rice types, respectively. Additionally, the original and cross-validated classification of rice DOM levels showed that, overall, 93.8% and 92.6% of rice samples were correctly classified. Our findings reveal variations in the nutraceutical levels and antioxidant activities in rice grains based on the rice type and DOM, which can help improve the nutritional evaluation of human-health-promoting rice grains.
The Impact of Plant-Based Coatings in “ROCHA” Pear Preservation during Cold Storage: A Metabolomic Approach
Authors: Fonseca Alexandre M. A. , Dias Cindy , Amaro Ana L. , Isidoro Nélson , Pintado Manuela , Silvestre Armando J. D. , Rocha Sílvia M.
Published: September 2020

Although new storage technologies have been emerging in recent years, preservation of pear (Pyrus communis L.) remains a challenge for suppliers. Maintenance of desired organoleptic properties throughout cold storage using non-chemical strategies has been investigated and the use of edible coatings has shown potential to delay fruit quality deterioration during cold storage. Thus, the objective of this study is to evaluate the impact of pectin coatings including plant extracts, in “Rocha” pear (Pyrus communis L. cv. Rocha) preservation. A four-month pilot scale assay was performed in both dynamic controlled atmosphere (DCA) (−0.5 °C, 0.5% O2, and 0.4% CO2) and normal atmospheric (NA) conditions (2 °C). For each storage condition, the following three coatings were tested: pectin (3% w/v) (PCT), pectin (3% w/v) + strawberry tree leaves extract (9.5 mg/mL) (CT1), and pectin (3% w/v) + apple pomace extract (16 mg/mL) (CT2). Volatile compounds, potentially related to aroma or ripening status of “Rocha” pear, were monitored alongside with conjugated trienols (CTs) and maturity parameters. The combination of DCA conditions and the application of pectin coatings were able to reduce the release of Rocha pear volatiles associated with ripening status, (particularly esters and sesquiterpenes), as well as reduce CTs, which could contribute to the preservation of Rocha pear for longer periods.
Non-Destructive Quality Assessment of Tomato Paste by Using Portable Mid-Infrared Spectroscopy and Multivariate Analysis
Authors: Aykas Didem Peren , Rodrigues Borba Karla , Rodriguez-Saona Luis E.
Published: September 2020

This research aims to provide simultaneous predictions of tomato paste’s multiple quality traits without any sample preparation by using a field-deployable portable infrared spectrometer. A total of 1843 tomato paste samples were supplied by four different leading tomato processors in California, USA, over the tomato seasons of 2015, 2016, 2017, and 2019. The reference levels of quality traits including, natural tomato soluble solids (NTSS), pH, Bostwick consistency, titratable acidity (TA), serum viscosity, lycopene, glucose, fructose, ascorbic acid, and citric acid were determined by official methods. A portable FT-IR spectrometer with a triple-reflection diamond ATR sampling system was used to directly collect mid-infrared spectra. The calibration and external validation models were developed by using partial least square regression (PLSR). The evaluation of models was conducted on a randomly selected external validation set. A high correlation (RCV = 0.85–0.99) between the reference values and FT-IR predicted values was observed from PLSR models. The standard errors of prediction were low (SEP = 0.04–35.11), and good predictive performances (RPD = 1.8–7.3) were achieved. Proposed FT-IR technology can be ideal for routine in-plant assessment of the tomato paste quality that would provide the tomato processors with accurate results in shorter time and lower cost.
Global Profiling of Dynamic Alternative Splicing Modulation in Arabidopsis Root upon Ralstonia solanacearum Infection
Authors: Qin Ning , Zhang Ruize , Zhang Mancang , Niu Yang , Fu Shouyang , Wang Yisa , Wang Dongdong , Chen Yue , Zhao Cuizhu , Chen Qin , Lu Haibin
Published: September 2020

Alternative splicing (AS) is an important mechanism by which eukaryotes regulate transcription and protein diversity. The dynamic changes in AS that occur on a genome-wide scale during interactions between plant roots and pathogens remain unknown. Here, we used the interaction between Arabidopsis and Ralstonia solanacearum as a model to explore the AS changes that take place during the response of roots to infection by means of high-throughput RNA-sequencing. We showed that dynamic changes in AS occur much earlier than changes at the level of transcription during R.solanacearum infection. Comparing genes that are regulated at the transcriptional and AS levels indicated that there are few common genes between differentially spliced genes (DSGs) and differentially expressed genes (DEGs). The functional gene ontology (GO) analysis identified that the enriched GO terms for the DSGs were different from those of the DEGs. The DSGs were over-represented in GO terms associated with post-transcriptional and translational regulations, suggesting that AS may act on RNA stability and during post-translation, thus affecting the output of plant defense molecules. Meanwhile, changes in DSGs were infection stage-specific. Furthermore, the nucleotide binding domain and leucine-rich repeat proteins and receptor-like kinases, key regulators in plant immunity, were shown to undergo dynamic changes in AS in response to R. solanacearum. Taken together, AS, along with transcription, modulates plant root defense to R. solanacearum through transcriptome reprogramming.
Evolutionary Models of the Cenozoic Basins of Central-Western Mediterranean Area: A Review of Methodological Approaches
Authors: Guerrera Francesco , Martín-Martín Manuel , Tramontana Mario
Published: September 2020

In the last 40 years, several models based on very different methodological approaches have been proposed to interpret the complex geodynamic evolution of the central-western Mediterranean area and, in particular, of the Cenozoic basins. The persistence of numerous interpretations and still-open problems resulted in the proliferation of very different models. The reconstructions presented are highly influenced by difficulties often encountered in considering constraints introduced by models built by means of completely different methodological approaches. For example, major difficulties can arise in integrating data from individual classical disciplines (i.e., geology, stratigraphy, geophysics, tectonics, magmatology and plate kinematics) with those resulting from the use of modern technologies (i.e., digital processing, uses of software, field observations using drones, etc.) and generally aimed to support specific topics. These considerations lead researchers to believe that a multidisciplinary approach would always be auspicious for these studies, because a greater control of the reconstruction of geologic and geodynamic events, and, therefore, for resulting models, would be ensured. After some considerations about different types of literature models based on specific investigation methodologies, the updating of a recently presented evolutionary model is proposed by attempting to integrate as much data as possible about the Cenozoic basins of the central-western Mediterranean area.
Identifying Predictive Characteristics of Opioid Medication Use among a Nationally Representative Sample of United States Older Adults with Pain and Comorbid Hypertension or Hypercholesterolemia
Authors: Axon David R. , Vaffis Shannon , Marupuru Srujitha
Published: September 2020

The prevalence of older adults with pain and comorbid cardiovascular conditions is increasing in the United States (U.S.). This retrospective, cross-sectional database study used 2017 Medical Expenditure Panel Survey data and hierarchical logistic regression models to identify predictive characteristics of opioid use among a nationally representative sample of older U.S. adults (aged ≥50 years) with pain in the past four weeks and comorbid hypertension (pain–hypertension group) or hypercholesterolemia (pain–hypercholesterolemia group). The pain–hypertension group included 2733 subjects (n = 803 opioid users) and the pain–hypercholesterolemia group included 2796 subjects (n = 795 opioid users). In both groups, predictors of opioid use included: White race versus others, Hispanic versus non-Hispanic ethnicity, 1 versus ≥5 chronic conditions, little/moderate versus quite a bit/extreme pain, good versus fair/poor perceived mental health, functional limitation versus no functional limitation, smoker versus non-smoker, and Northeast versus West census region. In addition, Midwest versus West census region was a predictor in the pain–hypertension group, and 4 versus ≥5 chronic conditions was a predictor in the pain–hypercholesterolemia group. In conclusion, several characteristics of older U.S. adults with pain and comorbid hypertension or hypercholesterolemia were predictive of opioid use. These characteristics could be addressed to optimize individuals’ pain management and help address the opioid overdose epidemic.
It Takes Time to Unravel the Ecology of War in Gaza, Palestine: Long-Term Changes in Maternal, Newborn and Toddlers’ Heavy Metal Loads, and Infant and Toddler Developmental Milestones in the Aftermath of the 2014 Military Attacks
Authors: Baraquoni Nabil al , Qouta Samir R. , Vänskä Mervi , Diab Safwat Y. , Punamäki Raija-Leena , Manduca Paola
Published: September 2020

Toxicant, teratogen and carcinogen metal war remnants negatively affect human health. The current study analyzes, first, the persistence of heavy metal contamination in newborn hair in four cohorts across time in Gaza Palestine; second, the change in mothers’ and infants’ heavy metal contamination from birth to toddlerhood; and third, the impact of heavy metal contamination on infants’ and toddlers’ growth and development. The hair of newborns was analyzed for twelve heavy metals by Inductively Coupled Plasma Mass Spectrometry (ICP/MS) in cohorts recruited at delivery in 2011, 2015, 2016, and 2018–2019. In the 2015 cohort, mothers’ hair samples were taken at delivery, and toddlers and mothers hair were also analyzed 18 months later. Growth levels of infants at six months and toddlers at 18 months were assessed according to World Health Organization (WHO) standards according to a mother report and pediatric check-up, respectively. 1. The level of metal contamination in utero was persistently high across 8 years, 2011, 2015, 2016, 2019, following three major military attacks (2009, 2012, 2014). 2. The 2015 cohort babies exposed in utero to attacks in 2014 at six months showed association of high load at birth in mother of arsenic and in newborn of barium with underweight, of barium and molybdenum in newborn with stunting. 3. Eighteen months after birth, toddlers had a higher level of metals in hairs than when they were born, while, in their mothers, such levels were similar to those at delivery, confirming persistence in the environment of war remnants. Underweight and stunting, both in infants and toddlers, were higher than reported for previous years, as well as being progressive within the cohort. Severe environmental factors, metal contamination and food insecurity put Gaza’s infant health at risk.
New Risk Situations Related to Low Noise from Electric Vehicles: Perception of Workers as Pedestrians and Other Vehicle Drivers
Authors: Pardo-Ferreira María Carmen , Torrecilla-García Juan Antonio , Heras-Rosas Carlos de las , Rubio-Romero Juan Carlos
Published: September 2020

Sales of electric and hybrid electric vehicles are increasing steadily worldwide, and consequently their presence increases in city areas. At low speeds, the low levels of noise produced by these vehicles could become a new risk factor for road users. However, the magnitude of the risk has not been accurately determined. In addition, its inclusion in the work environment could pose a new risk that should be managed. Thus, in relation to low noise levels of electric and hybrid vehicles, this study aimed to characterise the risk situations and determine the risk perception of workers as pedestrians and internal combustion engine vehicle drivers coming into contact with these vehicles. The data were extracted from 417 questionnaires filled out by the employees of public service companies who come into contact with electric and hybrid vehicles during their working day in the city of Málaga, in the region of Andalusia, Spain. According to the experiences reported, it seems that the risk due to the low noise levels of electric vehicles is moderate and does not reach alarming levels. These risk situations usually occurred in low speed urban areas, particularly when crossing the road, or in semi-pedestrian areas. Almost half the respondents considered that the electric vehicle poses a risk to other road users because it is more difficult to hear, and they believe it likely that other road users could be injured. Despite that risk, pedestrians did not change their way of walking or moving around the parking areas and other areas of the company. Electric and hybrid electric cars are now required to produce sound when travelling at low speeds. Nevertheless, the effectiveness of this measure should be assessed once implemented and future research should explore alternative non-acoustic measures.
Children in South Africa with and without Intellectual Disabilities’ Rating of Their Frequency of Participation in Everyday Activities
Authors: Samuels Alecia , Dada Shakila , Van Niekerk Karin , Arvidsson Patrik , Huus Karina
Published: September 2020

In a low-and middle-income country (LMIC) such as South Africa, not much is known about how children with intellectual disabilities (ID) participate in everyday activities, as no studies to date have compared their participation to peers without ID from the same background. Using a newly developed, contextually valid measure of participation, Picture my Participation (PmP), 106 children with (73) and without ID (33), rated their frequency of participation in activities of daily living. Previous international research has established that children with ID tend to participate less frequently than children without ID in everyday activities outside of the school setting. However, much of this research is based on proxy ratings from caregivers rather than children with ID themselves. There is a growing body of evidence that suggests children with disabilities have uniquely different views of their own participation than their caregivers. The existing research evidence is also delimited to studies conducted predominantly in high income contexts (HICSs). Since it is universally acknowledged that participation patterns are affected by the environment, it is important to evaluate the generalizability of the current evidence to LMICs. The current study found that there were many similar patterns of participation between the two groups although significant differences were noted in social, community, leisure and self-care activities. We compare these results to findings from studies conducted in HICs and find that there are similarities but also differences across contexts. This study highlights the importance of gaining a child’s perspective of participation and understanding how intellectual disability can affect participation relative to peers without ID in LMICS.
The Participation of Children with Intellectual Disabilities: Including the Voices of Children and Their Caregivers in India and South Africa
Authors: Dada Shakila , Bastable Kirsty , Schlebusch Liezl , Halder Santoshi
Published: September 2020

There is a shortage of research on the participation of children with intellectual disabilities from middle-income countries. Also, most child assessments measure either the child’s or the caregiver’s perceptions of participation. Participation, however, is an amalgamation of both perspectives, as caregivers play a significant role in both accessing and facilitating opportunities for children’s participation. This paper reports on both perceptions—those of children with intellectual disabilities and those of their caregiver, in India and South Africa. A quantitative group comparison was conducted using the Children’s Assessment of Participation and Enjoyment (CAPE) that was translated into Bengali and four South African languages. One hundred child–caregiver dyads from India and 123 pairs from South Africa participated in the study. The results revealed interesting similarities and differences in participation patterns, both between countries and between children and their caregivers. Differences between countries were mostly related to the intensity of participation, with whom, and where participation occurred. Caregiver and child reports differed significantly regarding participation and the enjoyment of activities. This study emphasises the need for consideration of cultural differences when examining participation and suggests that a combined caregiver-and-child-reported approach may provide the broadest perspective on children’s participation.
Associating COVID-19 Severity with Urban Factors: A Case Study of Wuhan
Authors: Li Xin , Zhou Lin , Jia Tao , Peng Ran , Fu Xiongwu , Zou Yuliang
Published: September 2020

Wuhan encountered a serious attack in the first round of the coronavirus disease 2019 (COVID-19) pandemic, which has resulted in a public health social impact, including public mental health. Based on the Weibo help data, we inferred the spatial distribution pattern of the epidemic situation and its impacts. Seven urban factors, i.e., urban growth, general hospital, commercial facilities, subway station, land-use mixture, aging ratio, and road density, were selected for validation with the ordinary linear model, in which the former six factors presented a globally significant association with epidemic severity. Then, the geographically weighted regression model (GWR) was adopted to identify their unevenly distributed effects in the urban space. Among the six factors, the distribution and density of major hospitals exerted significant effects on epidemic situation. Commercial facilities appear to be the most prevalently distributed significant factor on epidemic situation over the city. Urban growth, in particular the newly developed residential quarters with high-rise buildings around the waterfront area of Hanyang and Wuchang, face greater risk of the distribution. The influence of subway stations concentrates at the adjacency place where the three towns meet and some near-terminal locations. The aging ratio of the community dominantly affects the hinterland of Hankou to a broader extent than other areas in the city. Upon discovering the result, a series of managerial implications that coordinate various urban factors were proposed. This research may contribute toward developing specific planning and design responses for different areas in the city based on a better understanding of the occurrence, transmission, and diffusion of the COVID-19 epidemic in the metropolitan area.
Spatial-Temporal Analysis and Driving Factors Decomposition of (De)Coupling Condition of SO2 Emissions in China
Authors: Wang Yue , Shi Lei , Chen Di , Tan Xue
Published: September 2020

China has a fast-growing economy and is one of the top three sulfur dioxide (SO2) emitters in the world. This paper is committed to finding efficient ways for China to reduce SO2 emissions with little impact on its socio-economic development. Data of 30 provinces in China from 2000 to 2017 were collected to assess the decoupling relationship between economic growth and SO2 emissions. The Tapio method was used. Then, the temporal trend of decoupling was analyzed and the Moran Index was introduced to test spatial autocorrelation of the provinces. To concentrate resources and improve the reduction efficiency, a generalized logarithmic mean Divisia index improved by the Cobb–Douglas function was applied to decompose drivers of SO2 emissions and to identify the main drivers. Results showed that the overall relationship between SO2 emissions and economic growth had strong decoupling (SD) since 2012; provinces, except for Liaoning and Guizhou, have reached SD since 2015. The decoupling indexes of neighboring provinces had spatial dependence at more than 95% certainty. The main positive driver was the proportion of the secondary sector of the economy and the main negative drivers were related to energy consumption and investment in waste gas treatment. Then, corresponding suggestions for government and enterprises were made.
GIS-Based Emotional Computing: A Review of Quantitative Approaches to Measure the Emotion Layer of Human–Environment Relationships
Authors: Huang Yingjing , Fei Teng , Kwan Mei-Po , Kang Yuhao , Li Jun , Li Yizhuo , Li Xiang , Bian Meng
Published: September 2020

In recent years, with the growing accessibility of abundant contextual emotion information, which is benefited by the numerous georeferenced user-generated content and the maturity of artificial intelligence (AI)-based emotional computing technics, the emotion layer of human–environment relationship is proposed for enriching traditional methods of various related disciplines such as urban planning. This paper proposes the geographic information system (GIS)-based emotional computing concept, which is a novel framework for applying GIS methods to collective human emotion. The methodology presented in this paper consists of three key steps: (1) collecting georeferenced data containing emotion and environment information such as social media and official sites, (2) detecting emotions using AI-based emotional computing technics such as natural language processing (NLP) and computer vision (CV), and (3) visualizing and analyzing the spatiotemporal patterns with GIS tools. This methodology is a great synergy of multidisciplinary cutting-edge techniques, such as GIScience, sociology, and computer science. Moreover, it can effectively and deeply explore the connection between people and their surroundings with the help of GIS methods. Generally, the framework provides a standard workflow to calculate and analyze the new information layer for researchers, in which a measured human-centric perspective onto the environment is possible.
Random Forest-Based Landslide Susceptibility Mapping in Coastal Regions of Artvin, Turkey
Authors: Akinci Halil , Kilicoglu Cem , Dogan Sedat
Published: September 2020

Natural disasters such as landslides often occur in the Eastern Black Sea region of Turkey owing to its geological, topographical, and climatic characteristics. Landslide events occur nearly every year in the Arhavi, Hopa, and Kemalpaşa districts located on the Black Sea coast in the Artvin province. In this study, the landslide susceptibility map of the Arhavi, Hopa, and Kemalpaşa districts was produced using the random forest (RF) model, which is widely used in the literature and yields more accurate results compared with other machine learning techniques. A total of 10 landslide-conditioning factors were considered for the susceptibility analysis, i.e., lithology, land cover, slope, aspect, elevation, curvature, topographic wetness index, and distances from faults, drainage networks, and roads. Furthermore, 70% of the landslides on the landslide inventory map were used for training, and the remaining 30% were used for validation. The RF-based model was validated using the area under the receiver operating characteristic (ROC) curve. Evaluation results indicated that the success and prediction rates of the model were 98.3% and 97.7%, respectively. Moreover, it was determined that incorrect land-use decisions, such as transforming forest areas into tea and hazelnut cultivation areas, induce the occurrence of landslides.
Adenosine A2a Receptor Stimulation Attenuates Ischemia-Reperfusion Injury and Improves Survival in A Porcine Model of DCD Liver Transplantation
Authors: Czigany Zoltan , Craigie Eve Christiana , Lurje Georg , Song Shaowei , Yonezawa Kei , Yamamoto Yuzo , Minor Thomas , Tolba René Hany
Published: September 2020

Orthotopic liver transplantation (OLT) using allografts from donation after circulatory death (DCD) is potentially associated with compromised clinical outcomes due to ischemia-reperfusion injury (IRI)-induced organ damage and graft-related complications. The aim of this study was to provide in vivo data on the effects of adenosine A2a receptor stimulation in a clinically relevant large animal model of DCD liver transplantation. Cardiac arrest was induced in German Landrace pigs (n = 10; 20–25 kg). After 30 min of warm ischemia, the donor liver was retrieved following a cold flush with 3 L of histidine-tryptophan-ketoglutarate-HTK solution. Animals of the treatment group (n = 5/group) received a standard dose of the selective adenosine receptor agonist CGS 21680 added to the cold flush. All grafts were stored for 4.5 h at 4 °C in HTK-solution before OLT. Hepatocellular injury, apoptosis, protein kinase A-PKA activity, graft microcirculation, liver function, and animal survival were assessed. Compared to untreated livers, adenosine A2a receptor stimulation resulted in improved tissue microcirculation (103% ± 5% vs. 38% ± 4% compared to baseline; p < 0.05), accelerated functional recovery of the graft (indocyanine green-plasma disappearance rate (ICG-PDR) of 75% ± 18% vs. 40% ± 30% after 3 h), increased PKA activity ratio (56% ± 3% vs. 32% ± 3%; p < 0.001 after 1 h), and consequently reduced tissue necrosis and apoptosis. The potent protective effects were clinically manifested in significantly improved survival in the treatment group after 72 h (100% vs. 40%; p = 0.04). The ex vivo administration of adenosine A2a receptor agonist during the back-table flush mitigates IRI-mediated tissue damage and improves functional graft recovery and survival in a large animal model of DCD liver transplantation.
Protein Biomarkers for the Diagnosis of Alzheimer’s Disease at Different Stages of Neurodegeneration
Authors: Pérez Mar , Hernández Félix , Avila Jesús
Published: September 2020

Mainly obtained from familial Alzheimer’s disease patients’ data, we know that some features of the neurodegenerative start several years before the appearance of clinical symptoms. In this brief review, we comment on some molecular and cellular markers appearing at different stages of the disease, before or once the clinical symptoms are evident. These markers are present in biological fluids or could be identified by image techniques. The combined use of molecular and cellular markers will be of interest to determine the development of the different phases of the disease.
Circulating miRNAs in HER2-Positive and Triple Negative Breast Cancers: Potential Biomarkers and Therapeutic Targets
Authors: Gupta Ishita , Rizeq Balsam , Vranic Semir , Moustafa Ala-Eddin Al , Al Farsi Halema
Published: September 2020

Breast cancer is one of the most prevalent diseases among women worldwide and is highly associated with cancer-related mortality. Of the four major molecular subtypes, HER2-positive and triple-negative breast cancer (TNBC) comprise more than 30% of all breast cancers. While the HER2-positive subtype lacks estrogen and progesterone receptors and overexpresses HER2, the TNBC subtype lacks estrogen, progesterone and HER2 receptors. Although advances in molecular biology and genetics have substantially ameliorated breast cancer disease management, targeted therapies for the treatment of estrogen-receptor negative breast cancer patients are still restricted, particularly for TNBC. On the other hand, it has been demonstrated that microRNAs, miRNAs or small non-coding RNAs that regulate gene expression are involved in diverse biological processes, including carcinogenesis. Moreover, circulating miRNAs in serum/plasma are among the most promising diagnostic/therapeutic tools as they are stable and relatively easy to quantify. Various circulating miRNAs have been identified in several human cancers including specific breast cancer subtypes. This review aims to discuss the role of circulating miRNAs as potential diagnostic and prognostic biomarkers as well as therapeutic targets for estrogen-receptor negative breast cancers, HER2+ and triple negative.
Advancement of Nanobiomaterials to Deliver Natural Compounds for Tissue Engineering Applications
Authors: Kumar Sathish Sundar Dhilip , Abrahamse Heidi
Published: September 2020

Recent advancement in nanotechnology has provided a wide range of benefits in the biological sciences, especially in the field of tissue engineering and wound healing. Nanotechnology provides an easy process for designing nanocarrier-based biomaterials for the purpose and specific needs of tissue engineering applications. Naturally available medicinal compounds have unique clinical benefits, which can be incorporated into nanobiomaterials and enhance their applications in tissue engineering. The choice of using natural compounds in tissue engineering improves treatment modalities and can deal with side effects associated with synthetic drugs. In this review article, we focus on advances in the use of nanobiomaterials to deliver naturally available medicinal compounds for tissue engineering application, including the types of biomaterials, the potential role of nanocarriers, and the various effects of naturally available medicinal compounds incorporated scaffolds in tissue engineering.
Erythropoietin Mimetic Peptide (pHBSP) Corrects Endothelial Dysfunction in a Rat Model of Preeclampsia
Authors: Korokin Mikhail , Gureev Vladimir , Gudyrev Oleg , Golubev Ivan , Korokina Liliya , Peresypkina Anna , Pokrovskaia Tatiana , Lazareva Galina , Soldatov Vladislav , Zatolokina Mariya , Pobeda Anna , Avdeeva Elena , Beskhmelnitsyna Evgeniya , Denisyuk Tatyana , Avdeeva Natalia , Bushueva Olga , Pokrovskii Mikhail
Published: September 2020

Preeclampsia is a severe disease of late pregnancy. Etiological factors and a pathogenetic pattern of events still require significant clarification, but it is now recognized that a large role is played by placentation disorders and emerging endothelial dysfunction. The administration of short-chain peptides mimicking the spatial structure of the B erythropoietin chain may become one of the directions of searching for new drugs for preeclampsia prevention and therapy. Simulation of ADMA-like preeclampsia in Wistar rats was performed by the administration of a non-selective NOS blocker L-NAME from the 14th to 20th day of pregnancy. The administration of the pHBSP at the doses of 10 µg/kg and 250 µg/kg corrected the established morphofunctional disorders. The greatest effect was observed at a dose of 250 µg/kg. There was a decrease in systolic and diastolic blood pressure by 31.2 and 32.8%, respectively (p < 0.0001), a decrease in the coefficient of endothelial dysfunction by 48.6% (p = 0.0006), placental microcirculation increased by 82.8% (p < 0.0001), the NOx concentration was increased by 42,6% (p = 0.0003), the greater omentum edema decreased by 11.7% (p = 0.0005) and proteinuria decreased by 76.1% (p < 0.0002). In addition, there was an improvement in the morphological pattern of the fetoplacental complex and the ratio of BAX to Bcl-2 expression which characterizes the apoptotic orientation of the cells.
Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease
Authors: Miró Lluïsa , Amat Concepció , Rosell-Cardona Cristina , Campbell Joy M. , Polo Javier , Pérez-Bosque Anna , Moretó Miquel
Published: September 2020

Dietary supplementation with spray-dried porcine plasma (SDP) can modulate the immune response of gut-associated lymphoid tissue. SDP supplementation reduces acute mucosal inflammation, as well as chronic inflammation associated with aging. The aim of this study was to analyze if SDP supplementation could ameliorate colitis in a genetic mouse model of inflammatory bowel disease (IBD). Wild-type mice and Mdr1a knockout (KO) mice were administered a control diet or an SDP-supplemented diet from day 21 (weaning) until day 56. The histopathological index, epithelial barrier, and intestinal immune system were analyzed in the colonic mucosa. KO mice had higher epithelial permeability, increased Muc1 and Muc4 expression, and lower abundance of E-cadherin and Muc2 (all p < 0.001). SDP prevented these effects (all p < 0.05) and decreased the colonic inflammation observed in KO mice, reducing neutrophil and monocyte infiltration and activation and the percentage of activated T helper lymphocytes in the colonic mucosa (all p < 0.05). SDP also diminished proinflammatory cytokine expression and increased the anti-inflammatory IL-10 concentration in the colonic mucosa (all p < 0.05). In conclusion, dietary supplementation with SDP enhances colon barrier function and reduces mucosal inflammation in a mouse model of IBD.
Changes in Nuclear Shape and Gene Expression in Response to Simulated Microgravity Are LINC Complex-Dependent
Authors: Neelam Srujana , Richardson Brian , Barker Richard , Udave Ceasar , Gilroy Simon , Cameron Mark J. , Levine Howard G. , Zhang Ye
Published: September 2020

Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. In our study, we used a simulated microgravity device, a 3-D clinostat (Gravite), to investigate whether the LINC complex mediates cellular responses to the simulated microgravity environment. We show that nuclear shape and differential gene expression are both responsive to simulated microgravity in a LINC-dependent manner and that this response changes with the duration of exposure to simulated microgravity. These LINC-dependent genes likely represent elements normally regulated by the mechanical forces imposed by gravity on Earth.
Development of a High-Efficacy Reprogramming Method for Generating Human Induced Pluripotent Stem (iPS) Cells from Pathologic and Senescent Somatic Cells
Authors: Tanaka Naomichi , Kato Hidemasa , Tsuda Hiromi , Sato Yasunori , Muramatsu Toshihiro , Iguchi Atsushi , Nakajima Hiroyuki , Yoshitake Akihiro , Senbonmatsu Takaaki
Published: September 2020

Induced pluripotent stem (iPS) cells are a type of artificial pluripotent stem cell induced by the epigenetic silencing of somatic cells by the Yamanaka factors. Advances in iPS cell reprogramming technology will allow aging or damaged cells to be replaced by a patient’s own rejuvenated cells. However, tissue that is senescent or pathologic has a relatively low reprogramming efficiency as compared with juvenile or robust tissue, resulting in incomplete reprogramming; iPS cells generated from such tissue types do not have sufficient differentiation ability and are therefore difficult to apply clinically. Here, we develop a new reprogramming method and examine it using myofibroblasts, which are pathologic somatic cells, from patient skin tissue and from each of the four heart chambers of a recipient heart in heart transplant surgery. By adjusting the type and amount of vectors containing transcriptional factors for iPS cell reprogramming, as well as adjusting the transfection load and culture medium, the efficiency of iPS cell induction from aged patient skin-derived fibroblasts was increased, and we successfully induced iPS cells from myocardial fibroblasts isolated from the pathologic heart of a heart transplant recipient.
Comparative Effects of Particle Sizes of Cobalt Nanoparticles to Nine Biological Activities
Authors: Kong In Chul , Ko Kyung-Seok , Koh Dong-Chan , Chon Chul-Min
Published: September 2020

The differences in the toxicity of cobalt oxide nanoparticles (Co-NPs) of two different sizes were evaluated in the contexts of the activities of bacterial bioluminescence, xyl-lux gene, enzyme function and biosynthesis of β-galactosidase, bacterial gene mutation, algal growth, and plant seed germination and root/shoot growth. Each size of Co-NP exhibited a different level of toxicity (sensitivity) in each biological activity. No revertant mutagenic ratio (greater than 2.0) of Salmonella typhimurium TA 98 was observed under the test conditions in the case of gene-mutation experiments. Overall, the inhibitory effects on all five bacterial bioassays were greater than those on algal growth, seed germination, and root growth. However, in all cases, the small Co-NPs showed statistically greater (total average about two times) toxicity than the large Co-NPs, except in shoot growth, which showed no observable inhibition. These findings demonstrate that particle size may be an important physical factor determining the fate of Co-NPs in the environment. Moreover, combinations of results based on various biological activities and physicochemical properties, rather than only a single activity and property, would better facilitate accurate assessment of NPs’ toxicity in ecosystems.
Newborn Critical Congenital Heart Disease Screening Using Pulse Oximetry: Value and Unique Challenges in Developing Regions
Authors: Hom Lisa A. , Martin Gerard R.
Published: September 2020

Newborn screening for critical congenital heart disease (CCHD) is recommended for implementation in many developed countries as the standard of care. Efforts to implement this point of care screen in developing regions face unique barriers, and present important opportunities. The First Pan-African Newborn Screening Conference, held in Rabat in June 2019, incorporated a workshop dedicated specifically to identifying and discussing CCHD screening issues in the Middle East Northern Africa (MENA) region. The issues explored may be beneficial as part of the greater discussion of CCHD screening’s growing importance in developing regions around the world. Screening experts presented education and lessons learned from previous CCHD implementations, including a hands-on technical demonstration of CCHD screening. Children’s HeartLink, The Newborn Foundation, and Children’s National Hospital each presented on their experiences working with teams and pilot projects from around the world. Experience in implementation from Children’s Hospital Marrakesh was presented and highlighted some of the unique findings, challenges, and experiences of screening in Morocco. As developing regions investigate the implementation of CCHD screening using pulse oximetry either as part of research studies, pilots, regional studies, or as part of a nationally supported program, data to inform policymakers on the benefits of screening and specific needs for infrastructure development and resources are essential. This special issue contains initial lessons learned on newborn CCHD screening from a select number of developing countries, including Saudi Arabia and Morocco and regions such as Latin America.
A Fast Algorithm to Initialize Cluster Centroids in Fuzzy Clustering Applications
Authors: Cebeci Zeynel , Cebeci Cagatay
Published: September 2020

The goal of partitioning clustering analysis is to divide a dataset into a predetermined number of homogeneous clusters. The quality of final clusters from a prototype-based partitioning algorithm is highly affected by the initially chosen centroids. In this paper, we propose the InoFrep, a novel data-dependent initialization algorithm for improving computational efficiency and robustness in prototype-based hard and fuzzy clustering. The InoFrep is a single-pass algorithm using the frequency polygon data of the feature with the highest peaks count in a dataset. By using the Fuzzy C-means (FCM) clustering algorithm, we empirically compare the performance of the InoFrep on one synthetic and six real datasets to those of two common initialization methods: Random sampling of data points and K-means++. Our results show that the InoFrep algorithm significantly reduces the number of iterations and the computing time required by the FCM algorithm. Additionally, it can be applied to multidimensional large datasets because of its shorter initialization time and independence from dimensionality due to working with only one feature with the highest number of peaks.
Exploring Casual COVID-19 Data Visualizations on Twitter: Topics and Challenges
Authors: Trajkova Milka , Alhakamy A’aeshah , Cafaro Francesco , Vedak Sanika , Mallappa Rashmi , Kankara Sreekanth R.
Published: September 2020

Social networking sites such as Twitter have been a popular choice for people to express their opinions, report real-life events, and provide a perspective on what is happening around the world. In the outbreak of the COVID-19 pandemic, people have used Twitter to spontaneously share data visualizations from news outlets and government agencies and to post casual data visualizations that they individually crafted. We conducted a Twitter crawl of 5409 visualizations (from the period between 14 April 2020 and 9 May 2020) to capture what people are posting. Our study explores what people are posting, what they retweet the most, and the challenges that may arise when interpreting COVID-19 data visualization on Twitter. Our findings show that multiple factors, such as the source of the data, who created the chart (individual vs. organization), the type of visualization, and the variables on the chart influence the retweet count of the original post. We identify and discuss five challenges that arise when interpreting these casual data visualizations, and discuss recommendations that should be considered by Twitter users while designing COVID-19 data visualizations to facilitate data interpretation and to avoid the spread of misconceptions and confusion.
Long-Term Advantages of Ovarian Reserve Maintenance and Follicle Development Using Adipose Tissue-Derived Stem Cells in Ovarian Tissue Transplantation
Authors: Cacciottola Luciana , Nguyen Thu Y. T. , Chiti Maria C. , Camboni Alessandra , Amorim Christiani A. , Donnez Jacques , Dolmans Marie-Madeleine
Published: September 2020

(1) Background: Ovarian tissue transplantation with adipose tissue-derived stem cells (ASCs) has been shown to enhance graft vascularization and increase follicle survival after a short interval of 7 days. The aim of the present study was to investigate their long-term effects on primordial follicle pool maintenance and follicle development. (2) Methods: A total of 14 severe combined immunodeficient (SCID) mice were grafted with frozen-thawed human ovarian tissue with or without ASCs. Blood was taken monthly in order to quantify the anti-Müllerian hormone (AMH) and estradiol. After 6 months, all the grafts were retrieved and sent for histology and immunolabeling (AMH, AMH receptor II, estrogen receptors α and β, and c-kit/kit ligand). (3) Results: A significant upturn was observed in AMH and estradiol plasma levels 4 months after transplantation in both grafted groups. The primordial follicle pool was better preserved in the ASC group (41.86 ± 28.35) than in the standard transplantation group (9.65 ± 17.6, p < 0.05) compared to non-grafted controls (124.7 ± 140). (4) Conclusions: The use of ASCs prior to ovarian tissue transplantation yielded a larger primordial follicle pool and more physiological follicle distribution after long-term grafting. These findings suggested that ASC use might extend the ovarian tissue lifespan.
Bioaccumulation of Metals/Metalloids and Histological and Immunohistochemical Changes in the Tissue of the European Hake, Merluccius merluccius (Linnaeus, 1758) (Pisces: Gadiformes: Merlucciidae), for Environmental Pollution Assessment
Authors: Salvaggio Antonio , Pecoraro Roberta , Copat Chiara , Ferrante Margherita , Grasso Alfina , Scalisi Elena Maria , Ignoto Sara , Bonaccorsi Vincenza Serena , Messina Giuseppina , Lombardo Bianca Maria , Tiralongo Francesco , Brundo Maria Violetta
Published: September 2020

Pollution and other types of environmental stress do not spare marine environments, especially those affected by high industrial pressure. Fish, especially coastal species, are used for monitoring the marine environment because they are particularly efficient as bioindicators thanks to their ability to bioaccumulate and biomagnify along the trophic chain. The aim of this research is to evaluate the bioaccumulation and the indirect bioindication ability of the European Hake, Merluccius merluccius (Linnaeus, 1758), one of the most important commercial fish species of the Mediterranean Sea. Morphological and histological alterations of the main target organs, such as liver and gills, have been investigated and the results showed a steatosis in the hepatic tissue. The accumulation of heavy metals has been analyzed by inductively coupled plasma mass spectrometry and for several metals it was showed a different concentration in the two sexes. Moreover, the expression of metallothioneins 1 and Heat Shock Protein 70 has been assessed by immunohistochemistry and did not show high level of expression. We underline the importance of contamination evaluation in commercial fish species and the utilization of the ichthyofauna as bioindicator of environmental quality.
Funding Access and Innovation in Small Businesses
Authors: Harel Ronen , Schwartz Dafna , Kaufmann Dan
Published: September 2020

The study examined the extent to which lack of access to external funding constitutes a barrier to innovation for small businesses operating in traditional industries. The findings indicate that, these businesses do not view lack of access to funding as a barrier to innovation for any of the four types of innovation: product, process, marketing, or organizational. However, for most of the innovations they promoted, the levels of innovation were relatively low, and which naturally entails relatively low risk to businesses. The findings also indicate that, there is a relationship between product and marketing levels of innovation and lack of access to external funding. The study’s contribution lies in its focus on small businesses operating in traditional industries—businesses which though, essential to economic growth, have garnered less separate attention in the innovation sphere. The study points to a vicious circle in which these businesses do not promote innovation at high levels that would advance their own competitive advantage and require external funding. Because this funding is not within their reach, they continue promoting low-level innovation, and so on and so forth. The study may practically contribute by assisting policymakers as they draw plans dedicated to supporting innovation in small businesses.