bluuebunny/crossref_metadata_2025 huggingface dataset:
Medicinal Chemistry Friendliness of Pigments from Monascus-Fermented Rice and the Molecular Docking Analysis of Their Anti-Hyperlipidemia Properties
Authors: Sun Nina , Agyei Dominic , Ji Dawei
Published: November 2020

In this study, the physicochemical properties, pharmacokinetics properties, and drug-likeness of pigments from Monascus-fermented rice (Monascus pigments, MPs) were predicted in silico using SwissADME tool. In silico prediction of physicochemical properties showed that MPs had desirable lipophilic drug-like physicochemical properties including molecular weight (236 to 543), TPSA (44.76 to 179.77), lipophilicity (−0.81 to 4.14), and water solubility (−4.94 to −0.77). The pharmacokinetic properties of MPs (i.e., GIA, P-glycoprotein substrate, and CYP3A4 inhibitor) illustrated that most MPs had high intestinal absorption and bioavailability, but some MPs might cause pharmacokinetics-related drug–drug interactions. Following this, six main well-known MPs (monascin, ankaflavin, rubropunctatin, monascorubrin, rubropunctamine, monascorubramine) were selected for molecular docking with some enzyme receptors. The docking results were shown with the best molecular docking poses, and the interacting residues, number and distance of hydrogen bonds of the MPs and monacolin K (for docking with 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase)), or MPs and oleic acid (for docking with lipase). Dissociation constants showed that MPs had lower inhibitory potential for HMGR (compared with Monacolin K), and higher inhibitory potential for lipase. Individual pigments from Monascus-fermented rice, therefore, have the potential to be developed as drug candidates for controlling hyperlipidemia.
Hes5.9 Coordinate FGF and Notch Signaling to Modulate Gastrulation via Regulating Cell Fate Specification and Cell Migration in Xenopus tropicalis
Authors: Huang Xiao , Zhang Liyue , Yang Shanshan , Zhang Yongpu , Wu Mingjiang , Chen Peichao
Published: November 2020

Gastrulation drives the establishment of three germ layers and embryonic axes during frog embryonic development. Mesodermal cell fate specification and morphogenetic movements are vital factors coordinating gastrulation, which are regulated by numerous signaling pathways, such as the Wnt (Wingless/Integrated), Notch, and FGF (Fibroblast growth factor) pathways. However, the coordination of the Notch and FGF signaling pathways during gastrulation remains unclear. We identified a novel helix–loop–helix DNA binding domain gene (Hes5.9), which was regulated by the FGF and Notch signaling pathways during gastrulation. Furthermore, gain- and loss-of-function of Hes5.9 led to defective cell migration and disturbed the expression patterns of mesodermal and endodermal marker genes, thus interfering with gastrulation. Collectively, these results suggest that Hes5.9 plays a crucial role in cell fate decisions and cell migration during gastrulation, which is modulated by the FGF and Notch signaling pathways.
Mapping of Diabetes Susceptibility Loci in a Domestic Cat Breed with an Unusually High Incidence of Diabetes Mellitus
Authors: Balmer Lois , O’Leary Caroline Ann , Menotti-Raymond Marilyn , David Victor , O’Brien Stephen , Penglis Belinda , Hendrickson Sher , Reeves-Johnson Mia , Gottlieb Susan , Fleeman Linda , Vankan Dianne , Rand Jacquie , Morahan Grant
Published: November 2020

Genetic variants that are associated with susceptibility to type 2 diabetes (T2D) are important for identification of individuals at risk and can provide insights into the molecular basis of disease. Analysis of T2D in domestic animals provides both the opportunity to improve veterinary management and breeding programs as well as to identify novel T2D risk genes. Australian-bred Burmese (ABB) cats have a 4-fold increased incidence of type 2 diabetes (T2D) compared to Burmese cats bred in the United States. This is likely attributable to a genetic founder effect. We investigated this by performing a genome-wide association scan on ABB cats. Four SNPs were associated with the ABB T2D phenotype with p values <0.005. All exons and splice junctions of candidate genes near significant single-nucleotide polymorphisms (SNPs) were sequenced, including the genes DGKG, IFG2BP2, SLC8A1, E2F6, ETV5, TRA2B and LIPH. Six candidate polymorphisms were followed up in a larger cohort of ABB cats with or without T2D and also in Burmese cats bred in America, which exhibit low T2D incidence. The original SNPs were confirmed in this cohort as associated with the T2D phenotype, although no novel coding SNPs in any of the seven candidate genes showed association with T2D. The identification of genetic markers associated with T2D susceptibility in ABB cats will enable preventative health strategies and guide breeding programs to reduce the prevalence of T2D in these cats.
Assessment of the Dietary Intake of High-Rank Professional Male Football Players during a Preseason Training Week
Authors: Książek Anna , Zagrodna Aleksandra , Słowińska-Lisowska Małgorzata
Published: November 2020

A well-balanced diet is one of the main factors that may play a supportive role in enhancing acute training stimuli in optimal training adaptation. The aim of the present study was to examine the energy and macro- and micronutrient intake including and excluding supplements among top-level Polish football players during one week of the general preparatory period. In addition, the study looked at whether athletes consume carbohydrates in recommended amounts, depending on the completed training sessions. A total of 26 professional football players were included in the study. The preseason dietary intake was assessed using a 7-day estimated food record. The energy value of the diet and the amounts of the dietary ingredients were assessed using the software Dieta 6.0. The average consumption of energy, vitamin B2, vitamin C, vitamin E, folate, and calcium was lower than recommendations, and average intake of sodium and potassium was higher than the norm in the diets of the athletes. The results of this study do not confirm the justification for adding protein preparations to diets of the studied players. Furthermore, football players dietary carbohydrate intake was relatively low in comparison to requirements based on training loads. Based on our results we conclude that further work is necessary to reinforce education about nutritional habits and adjust nutritional strategies to individual needs to enhance athletic performance.
Erratum: Paolo Lauriola et al., Advancing Global Health through Environmental and Public Health Tracking, Int. J. Environ. Res. Public Health 2020, 17, 1976
Authors: Lauriola Paolo , Crabbe Helen , Behbod Behrooz , Yip Fuyuen , Medina Sylvia , Semenza Jan C. , Vardoulakis Sotiris , Kass Dan , Zeka Ariana , Khonelidze Irma , Ashworth Matthew , de Hoogh Kees , Shi Xiaoming , Staatsen Brigit , Knudsen Lisbeth E. , Fletcher Tony , Houthuijs Danny , Leonardi Giovanni S.
Published: November 2020

Due to an error during production, some contents of Table 1 are missing in the published paper [...]
An Improved Passive CR-39-Based Direct 222Rn/220Rn Progeny Detector
Authors: Hu Jun , Yang Guosheng , Kranrod Chutima , Iwaoka Kazuki , Hosoda Masahiro , Tokonami Shinji
Published: November 2020

An improved passive CR-39-based direct 222Rn/220Rn progeny detector with 3 detection channels was designed and tested in this study to measure and calculate equilibrium equivalent concentration (EEC) of both 222Rn and 220Rn without the equilibrium factor. A theoretical model was established to calculate the EEC with optimization. Subsequently, an exposure experiment was carried out to test the performance of this detector, and we compared the chamber experiment and the theoretical model by estimating and measuring various parameters. The deposition flux of progeny derived from the prediction agreed well with the value measured in the exposure chamber. The energy-weighted net track density (NTD) measured by this detector is much more reliable to reflect the linear relation between NTD and time-integrated EEC. Since the detector is sensitive to the exposure environmental condition, it is recommended to apply the detector to measure the EEC after its calibration in a typical indoor environment.
Effects of Cigarette Smoking on Cardiac Autonomic Responses: A Cross-Sectional Study
Authors: Makhoul Nadeen , Avivi Ishay , Barak Lanciano Sapir , Haber Kaptsenel Ella , Bishara Hana , Palacci Hagar , Chaiat Chen , Jacob Giris , Nussinovitch Udi
Published: November 2020

It has been suggested that some of the adverse, long-term cardiovascular outcomes of smoking are mediated by impaired autonomic nervous system (ANS) activity. Yet, this association is currently inconclusive. Heart rate variability (HRV) and the deep breathing test (DBT) represent common quantitative markers of ANS activity due to their simplicity and reliability. This large cross-sectional study was designed to assess the effect of active smoking on ANS function as manifested by HRV or DBT abnormalities. Electrocardiograms were recorded at rest for 5 min and during forced metronomic breathing. HRV and DBT were calculated according to accepted standards. Participants were divided into two groups based on current smoking status. The study included 242 healthy volunteers (196 nonsmokers and 46 smokers). There were no significant differences in age, sex, and BMI between groups. Cumulative smoking exposure burden (CSEB) for the study group was 5.3 ± 1.3 pack-years. Comparative analysis of HRV and DBT parameters according to smoking status revealed no significant differences between groups. Significant (p < 0.05), yet weak or moderate correlations (r < 0.7) were found between CSEB and abnormal change in HRV parameters consistent with sympathetic overactivity and decreased parasympathetic tone. In conclusion, smoking for a relatively short period in healthy adults does not seem to lead to significant impairment in ANS function. Yet, the consequences of smoking seem to be amplified when cumulative exposure burden increases.
Teachers’ Psychological Needs Satisfaction and Thwarting: Can They Explain Students’ Behavioural Engagement in Physical Education? A Multi-Level Analysis
Authors: Coterón Javier , Franco Evelia , Ocete Carmen , Pérez-Tejero Javier
Published: November 2020

Students’ engagement in Physical Education has been linked to several adaptive consequences. Even though the existing literature suggests that perceived autonomy support can predict engagement, research is scarce on how teachers’ antecedents might influence this behavioural outcome. This study sought to compare the influence of teachers’ basic psychological needs’ satisfaction and basic psychological needs’ thwarting on students’ behavioural engagement and on the relationship between perceived autonomy-support and the students’ behavioural engagement. The sample included 29 Physical Education teachers and 644 students who were taught by the participants teachers. Data were collected using both paper and online surveys and they were analysed using multilevel modelling techniques. The results revealed that, while teachers’ autonomy satisfaction might be significant in the explanation of students’ engagement (β = 0.33, p < 0.01), it seems that needs thwarting could be a better predictor of this outcome (β autonomy thwarting = −0.17, p < 0.01; β competence thwarting = −0.06, p < 0.05). These findings suggest the impact of certain external pressures on teachers’ practices which, in turn, might affect students’ behavioural outcomes.
Grappling with Gulf War Illness: Perspectives of Gulf War Providers
Authors: Kaimal Girija , Dieterich-Hartwell Rebekka
Published: November 2020

Background: Although the Gulf War occurred almost 30 years ago, the chronic symptoms of Gulf War illness (GWI), which include respiratory, gastrointestinal, and skin problems, as well as fatigue, pain, and mood alterations, currently affect over 200,000 veterans. Meanwhile, healthcare providers lack clear guidelines about how to best treat this illness. The objective in this study was to learn about the perceptions and experiences of healthcare providers of GWI veterans in terms of medical symptoms, resources for treatment, and quality of care. Methods: We interviewed 10 healthcare providers across the United States and subsequently conducted a qualitative grounded theory study which entailed both systematic data analysis and generating a grounded theory framework. Results: Our findings indicated multiple challenges for providers of veterans with GWI, including gaps in knowledge about GWI, lack of treatment options, absence of consistent communication within the Department of Veterans Affairs (VA) system, and personalized care that was limited to validation. Conclusion: While this study had several limitations, it supported the notion that healthcare providers have inadequate knowledge and awareness about GWI, which leads to continued uncertainty about how to best care for GWI veterans. This could be remedied by the creation of a comprehensive curriculum for a Massive Open Online Course (MOOC) to serve as an educational tool for those attending to this largely overlooked veteran population.
The Psychological Consequences of COVID-19 and Lockdown in the Spanish Population: An Exploratory Sequential Design
Authors: Hidalgo María Dolores , Balluerka Nekane , Gorostiaga Arantxa , Espada José Pedro , Santed Miguel Ángel , Padilla José Luis , Gómez-Benito Juana
Published: November 2020

The objectives of this study were to analyze the psychological impact of the COVID-19 pandemic and the lockdown in the Spanish population and to identify what population profiles were most affected. The study used a sequential exploratory design. In the qualitative phase, 40 participants were recruited based on theoretically relevant criteria and the saturation of the information provided by the interviews. In the quantitative phase, a large representative sample was applied. The universe considered was the adult population of Spain. A total of 6789 surveys were conducted. Both the analysis of the narratives of the interviews and the responses to the panel survey showed relevant changes in attitudes and mood swings compared to the period prior to lockdown. These changes include dysphoric moods (i.e., experiences of distress such as sadness/depression, anxiety, rage, feeling of unreality, worry, etc.) and also some euphoric moods (i.e., feelings of well-being, happiness, etc.). A higher number of women were affected than men and a greater increase was observed in younger people. The findings of the study may serve as a basis for detecting needs and providing psychological support, as the symptoms detected as the most common are key for the processes of screening at-risk individuals.
Improvement of Ficin-Based Inhibitive Enzyme Assay for Toxic Metals Using Response Surface Methodology and Its Application for Near Real-Time Monitoring of Mercury in Marine Waters
Authors: Uba Garba , Manogaran Motharasan , Gunasekaran Baskaran , Halmi Mohd Izuan Effendi , Shukor Mohd Yunus Abd
Published: November 2020

Potentially toxic metals pollution in the Straits of Malacca warrants the development of rapid, simple and sensitive assays. Enzyme-based assays are excellent preliminary screening tools with near real-time potential. The heavy-metal assay based on the protease ficin was optimized for mercury detection using response surface methodology. The inhibitive assay is based on ficin action on the substrate casein and residual casein is determined using the Coomassie dye-binding assay. Toxic metals strongly inhibit this hydrolysis. A central composite design (CCD) was utilized to optimize the detection of toxic metals. The results show a marked improvement for the concentration causing 50% inhibition (IC50) for mercury, silver and copper. Compared to one-factor-at-a-time (OFAT) optimization, RSM gave an improvement of IC50 (mg/L) from 0.060 (95% CI, 0.030–0.080) to 0.017 (95% CI, 0.016–0.019), from 0.098 (95% CI, 0.077–0.127) to 0.028 (95% CI, 0.022–0.037) and from 0.040 (95% CI, 0.035–0.045) to 0.023 (95% CI, 0.020–0.027), for mercury, silver and copper, respectively. A near-real time monitoring of mercury concentration in the Straits of Malacca at one location in Port Klang was carried out over a 4 h interval for a total of 24 h and validated by instrumental analysis, with the result revealing an absence of mercury pollution in the sampling site.
Sleep Disordered Breathing: An Epidemiological Study among Albanian Children and Adolescents
Authors: Abazi Yllka , Cenko Fabian , Cardella Marianna , Tafa Gjergji , Laganà Giuseppina
Published: November 2020

Sleep Disordered Breathing (SDB) comprises a group of diseases characterized by alterations in the frequency and/or depth of breathing during sleep. The aim of this study was to investigate the frequency of SDB in a group of Albanian children and adolescents and to describe its social, physiological, psychological, sleep-related, and anthropometric risk factors, in relation to the sociodemographic situation. A total of 6087 participants (mean age: 10.42 years, range: 6 to 15 years, 52.3% females and 47.7% males) attending public schools all over Albania took part in the cross-sectional study. On a sample of 6087 questionnaires distributed, 4702 (77.25% of the original sample) were returned and included in the study. High risk status for SDB was assessed using the Paediatric Sleep Questionnaire (PSQ). The prevalence of SDB was 7.9%. No statistically significant difference was found for gender at high risk for SBD. Compared to participants living in urban aeras (7.3%), participants living in rural areas (10.4%) reported significantly higher SDB prevalence rates. No other significant correlations were detected between the high-risk subjects and the age. The prevalence of the subjects at high risk of SBD obese participants (20.8%) was statistically higher than among nonobese ones (6.3%). SDB is highly prevalent in Albanian growing population and further prevalence studies are recommended.
Livestock Wastewater Treatment in Constructed Wetlands for Agriculture Reuse
Authors: Dias Sofia , Mucha Ana P. , Duarte Crespo Rute , Rodrigues Pedro , Almeida C. Marisa R.
Published: November 2020

The aim of this study focused on the evaluation of constructed wetlands (CWs) microcosms, on a laboratory scale, for the removal of metals from a pig industry effluent while maintaining effluent organic matter and nutrients levels for its later used as a fertilizer. CWs with different macrophytes (Phragmites australis and Typha latifolia) and different substrates (light expanded clay aggregate and lava rock) were tested. Results showed high removals of metals during CWs treatment, with removal rates reaching >80% for Cd, Cr, Cu, Fe, Mn, and Zn after 2 days of treatment in CWs planted with T. latifolia and >60% in CWs planted with P. australis. Significant differences were only found between substrates for Fe and Mn in CWs with P. australis. Removal of organic matter (through chemical oxygen demand (COD)) was >77%, with no significant differences between substrates or plants. Removals of ammonium and phosphate ions ranged between 59–84% and 32–92%, respectively, in CWs with P. australis and 62–75% and 7–68% in CWs with T. latifolia, with no significant differences between substrates. Overall, CWs showed potential to be efficient in removing toxic contaminants, as metals, while maintaining moderated levels of nutrients, allowing the use of reclaimed water in agriculture, namely as fertilizer. If one aims for a short CW treatment, CW planted with T. latifolia and expanded clay as substrate could be the more suitable choice.
Optimization of UHF RFID Five-Slotted Patch Tag Design Using PSO Algorithm for Biomedical Sensing Systems
Authors: Bouhassoune Ibtissame , Chehri Abdellah , Saadane Rachid , Minaoui Khalid
Published: November 2020

In this paper, a new flexible wearable radio frequency identification (RFID) five-shaped slot patch tag placed on the human arm is designed for ultra-high frequency (UHF) healthcare sensing applications. The compact proposed tag consists of a patch structure provided with five shaped slot radiators and a flexible substrate, which minimize the human body’s impact on the antenna radiation performance. We have optimized our designed tag using the particle swarm optimization (PSO) method with curve fitting within MATLAB to minimize antenna parameters to achieve a good return loss and an attractive radiation performance in the operating band. The PSO-optimized tag’s performance has been examined over the specific placement in some parts of the human body, such as wrist and chest, to evaluate the tag response and enable our tag antenna conception in wearable biomedical sensing applications. Finally, we have tested the robustness of this tag by evaluating its sensitivity as a function of the antenna radiator placement over the ground plane or by shaping the ground plane substrate for the tag’s position from the human body. Our numerical results show an optimal tag size with good matching features and promising read ranges near the human body.
Factors Associated with Dental Plaque, Gingivitis, and Caries in a Pediatric Population: A Records-Based Cross-Sectional Study
Authors: Davidovich Esti , Grender Julie , Zini Avi
Published: November 2020

This retrospective, cross-sectional study evaluated dental records of 1000 healthy children to determine factors associated with plaque, gingivitis, and caries. A logistic model for plaque and gingivitis (mild versus moderate/severe) and caries (yes/no) was carried out separately for each variable using the following potential factors: Age, Gender, Brush Type, Starting Age of Brushing, Brushing Frequency, and Bite Type. Data from 998 children (median age: 4 years, 10 months (range: 2.5–7 years)) were analyzed. Sixty-four percent were manual toothbrush users; 36% were oscillating-rotating electric toothbrush users. For plaque and gingivitis, but not caries, Brush Type was more impactful than Brushing Frequency. Age influenced the severity of plaque and gingivitis, with increases in the odds of having moderate/severe plaque or gingivitis associated with increasing age. The probability of caries increased until approximately age 5 and then decreased until age 7. Oscillating-rotating brush users were more likely to present with less plaque, gingivitis, and caries, with 6.0, 5.1, and 1.4 times greater odds of having mild (versus moderate/severe) plaque, less severe gingivitis, and being caries-free, respectively, than manual brush users. Similarly, brushing twice daily and starting brushing at an earlier age were associated with better oral health outcomes. Children with anterior bite abnormalities had increased odds of developing moderate/severe plaque and gingivitis than children with normal anterior bites. Gender was not a statistically significant factor associated with plaque, gingivitis, or caries. Children’s oral health is influenced by toothbrush type, starting age of brushing, compliance with twice-daily brushing, and bite abnormalities.
Impact of Matrix Metalloproteinase-11 Gene Polymorphisms on Biochemical Recurrence and Clinicopathological Characteristics of Prostate Cancer
Authors: Hsieh Chun-Yu , Chou Ying-Erh , Lin Chia-Yen , Wang Shian-Shiang , Chien Ming-Hsien , Tang Chih-Hsin , Lin Jian-Cheng , Wen Yu-Ching , Yang Shun-Fa
Published: November 2020

Prostate cancer is among the most common malignant tumors worldwide. Matrix metalloproteinase (MMP)-11 is involved in extracellular matrix degradation and remodeling and plays an essential role in cancer development and metastasis. This study investigated the association of MMP-11 polymorphisms with the clinicopathological characteristics and biochemical recurrence of prostate cancer. Five single-nucleotide polymorphisms (SNPs) of the MMP-11 were analyzed in 578 patients with prostate cancer through real-time polymerase chain reaction analysis. A prostate-specific antigen level of >10 ng/mL, Gleason grade groups 4 + 5, advanced tumor stage, lymph node metastasis, invasion, and high-risk D’Amico classification were significantly associated with biochemical recurrence in the patients (p < 0.001). MMP-11 rs131451 “TC + CC” polymorphic variants were associated with advanced clinical stage (T stage; p = 0.007) and high-risk D’Amico classification (p = 0.015) in patients with biochemical recurrence. These findings demonstrate that MMP-11 polymorphisms were not associated with prostate cancer susceptibility; however, the rs131451 polymorphic variant was associated with late-stage tumors and high-risk D’Amico classification in prostate cancer patients with biochemical recurrence. Thus, the MMP-11 SNP rs131451 may contribute to the tumor development in prostate cancer patients with biochemical recurrence.
Content Validation of an Instrument for the Assessment of School Teachers’ Levels of Knowledge of Diabetes through Expert Judgment
Authors: Luque-Vara Trinidad , Linares-Manrique Marta , Fernández-Gómez Elisabet , Martín-Salvador Adelina , Sánchez-Ojeda María Angustias , Enrique-Mirón Carmen
Published: November 2020

The objective of this study was to describe the content validation, through expert judgment, of a questionnaire for determining the level of knowledge that school teachers have of diabetes in order to design relevant educational interventions to improve the health of school-aged children. This psychometric study involved 15 experts who assessed each of the items in the instrument. The results revealed that the strength of agreement shown by the questionnaire ranged from substantial to almost perfect in its four dimensions, with the characteristics of “sufficiency” and “relevance” having the highest scores (0.982 and 0.903, respectively) based on the judgments made by the participating experts. Regarding statistical significance, the characteristics “sufficiency”, with p < 0.001, and “relevance”, with p = 0.001, were particularly relevant. The overall degree of understandability for the new version of the instrument was high (91.54%). The psychometric results obtained from validation of the “grado de conocimientos sobre diabetes en docentes del ámbito escolar” (Spanish for “level of knowledge of diabetes in school teachers”)—GCDDaE questionnaire through expert judgment and the pre-test indicate that it is recommended for use as it is both relevant and quick and easy to administer.
Higher Utilization of Social Services Is Associated with Higher Language Scores in Children from Deeply Impoverished Urban Families
Authors: Finkel Morgan A. , Troller-Renfree Sonya V. , Noble Kimberly G.
Published: November 2020

Language development has been consistently linked with socioeconomic status (SES), with children from lower SES backgrounds at higher risk for language delays. The objective of this study is to investigate the relationship between familial social service use and language development during the first year of life. Thirty-one low-income mothers and their infants were recruited from the New York metropolitan area. Mothers provided information about demographics and utilization of social services (Women, Infants, and Children (WIC), food stamps, Medicaid, and public housing). Infant language skills were assessed using the Preschool Language Scale. Multiple linear regressions were used to investigate the relationship between social service use and language skills. We found that the number of social services utilized was not an overall significant linear predictor of language skills. However, social service use interacted with poverty level to predict language skills. Specifically, for families living in deep poverty, higher service use significantly predicted higher infant language scores (β = 3.4, p = 0.005). These results suggest that social services may be an appropriate target to help narrow socioeconomic disparities in language development.
Cost-Effectiveness Analysis from a Randomized Controlled Trial of Tailored Exercise Prescription for Women with Breast Cancer with 8-Year Follow-Up
Authors: Gordon Louisa G. , Eakin Elizabeth G. , Spence Rosalind R. , Pyke Christopher , Bashford John , Saunders Christobel , Hayes Sandra C.
Published: November 2020

Studies show conflicting results on whether exercise interventions to improve outcomes for women with breast cancer are cost-effective. We modelled the long-term cost-effectiveness of the Exercise for Health intervention compared with usual care. A lifetime Markov cohort model for women with early breast cancer was constructed taking a societal perspective. Data were obtained from trial, epidemiological, quality of life, and healthcare cost reports. Outcomes were calculated from 5000 Monte Carlo simulations, and one-way and probabilistic sensitivity analyses. Over the cohort’s remaining life, the incremental cost for the exercise versus usual care groups were $7409 and quality-adjusted life years (QALYs) gained were 0.35 resulting in an incremental cost per QALY ratio of AU$21,247 (95% Uncertainty Interval (UI): Dominant, AU$31,398). The likelihood that the exercise intervention was cost-effective at acceptable levels was 93.0%. The incremental cost per life year gained was AU$8894 (95% UI Dominant, AU$11,769) with a 99.4% probability of being cost effective. Findings were most sensitive to the probability of recurrence in the exercise and usual care groups, followed by the costs of out-of-pocket expenses and the model starting age. This exercise intervention for women after early-stage breast cancer is cost-effective and would be a sound investment of healthcare resources.
Can Healthy Checkout Counters Improve Food Purchases? Two Real-Life Experiments in Dutch Supermarkets
Authors: Huitink Marlijn , Poelman Maartje P. , Seidell Jacob C. , Kuijper Lothar D. J. , Hoekstsra Trynke , Dijkstra Coosje
Published: November 2020

Most snacks displayed at supermarket checkouts do not contribute to a healthy diet. We investigated the effects of introducing healthier snack alternatives at checkouts in supermarkets on purchasing behavior. In Study 1, we investigated the effect of completely substituting less healthy with healthier snacks (one supermarket). In Study 2, we investigated the effect of placing and discounting healthier snacks while the less healthy snacks remain in place (two supermarkets). In both studies, the number of purchased snacks (per 1000 customers) was used as the outcome variable. Results for Study 1 showed that the absolute number of purchased checkout snacks was 2.4 times lower (95% confidence interval (CI): 1.9–2.7) when healthier snacks instead of less healthy snacks were placed at the supermarket checkouts. Results for Study 2 showed that when additional healthier snacks were placed near the checkouts, the absolute number of healthier purchased snacks increased by a factor of 2.1 (95% CI: 1.3–3.3). When additional healthier snacks were placed near the checkouts and discounted, the absolute number of healthier purchased snacks increased by a factor of 2.7 (95% CI: 2.0–3.6), although this was not statistically significant higher than placement only (ratio: 1.1, 95% CI: 0.7–1.9). Purchases of less healthy snacks did not decline, and even slightly increased, during the intervention period (ratio: 1.3, 95% CI: 1.1–1.5). If supermarkets want to promote healthier snack purchases, additional healthier products can be positioned near the checkouts. However, this does not discourages the purchase of less healthy snacks. Therefore, to discourage unhealthy snack purchases at supermarket checkouts, a total substitution of less healthy snacks with healthier alternatives is most effective.
Encouraging Patient Engagement Behaviors from the Perspective of Functional Quality
Authors: Mei Yi , Xu Xiaoyan , Li Xiaodong
Published: November 2020

Despite extensive research on how patient engagement behaviors (PEBs) are facilitated though explicit technical interventions in medical services, research on the encouragement of PEBs from the perspective of the service process is lacking. This study explores how functional quality dimensions (responsiveness, empathy, surroundings, and access) affect PEBs (compliance and loyalty) through a two-channel psychological mechanism (trust and satisfaction). This study tests the proposed model using survey data from two public hospitals in southeastern China and employs the partial least square (PLS) technique of structural equation modeling (SEM) to analyze the data. The results show that service providers’ responsiveness, empathy, and access affect patient compliance and loyalty through patient satisfaction; however, the effect of surroundings is not significant. The responsiveness and empathy of service staff affect PEBs through patient trust. Considering the high-contact professional nature of medical services, we call for more efforts toward improving service processes rather than simply relying on technical interventions. Specifically, hospitals and contact employees should devote time and effort to functional quality management in three dimensions, namely responsiveness, empathy, and access, to secure patient trust and satisfaction.
The Relative Importance of Globalization and Public Expenditure on Life Expectancy in Europe: An Approach Based on MARS Methodology
Authors: Martín Cervantes Pedro Antonio , Rueda López Nuria , Cruz Rambaud Salvador
Published: November 2020

Background: There has been a widespread debate about the overall impact of globalization on population, not just economically, but also in terms of health status. Moreover, the current health crisis is going to force governments to review the structure of the public budget to most effectively alleviate the negative economic and health effects on the population. Objective: The aim of this paper is to analyze the relative importance of globalization and the public budget composition—specifically the participation of public expenditure on healthcare, social services and environment in gross domestic product (GDP)—on life expectancy at birth in European countries during the period 1995–2017. Methods: The Multivariate Adaptive Regression Splines (MARS) methodology was applied to analyze the socioeconomic determinants of life expectancy at birth. Results: Our findings show that globalization has no relative importance as an explanatory variable of life expectancy in European countries, while government expenditure on social protection is the most relevant followed by public expenditure on health, gross national income per capita, education level of the population and public expenditure on environmental protection. Conclusion: European strategies intended to impact on health outcome should spend more attention to the composition of public budget.
Panoramic Mapping with Information Technologies for Supporting Engineering Education: A Preliminary Exploration
Authors: Lai Jhe-Syuan , Peng Yu-Chi , Chang Min-Jhen , Huang Jun-Yi
Published: November 2020

The present researchers took multistation-based panoramic images and imported the processed images into a virtual tour platform to create webpages and a virtual reality environment. The integrated multimedia platform aims to assist students in a surveying practice course. A questionnaire survey was conducted to evaluate the platform’s usefulness to students, and its design was modified according to respondents’ feedback. Panoramic photos were taken using a full-frame digital single-lens reflex camera with an ultra-wide-angle zoom lens mounted on a panoramic instrument. The camera took photos at various angles, generating a visual field with horizontal and vertical viewing angles close to 360°. Multiple overlapping images were stitched to form a complete panoramic image for each capturing station. Image stitching entails extracting feature points to verify the correspondence between the same feature point in different images (i.e., tie points). By calculating the root mean square error of a stitched image, we determined the stitching quality and modified the tie point location when necessary. The root mean square errors of nearly all panoramas were lower than 5 pixels, meeting the recommended stitching standard. Additionally, 92% of the respondents (n = 62) considered the platform helpful for their surveying practice course. We also discussed and provided suggestions for the improvement of panoramic image quality, camera parameter settings, and panoramic image processing.
Identification of Mitochondrial DNA (NUMTs) in the Nuclear Genome of Daphnia magna
Authors: Kowal Krzysztof , Tkaczyk Angelika , Pierzchała Mariusz , Bownik Adam , Ślaska Brygida
Published: November 2020

This is the first study in which the Daphnia magna (D. magna) nuclear genome (nDNA) obtained from the GenBank database was analyzed for pseudogene sequences of mitochondrial origin. To date, there is no information about pseudogenes localized in D. magna genome. This study aimed to identify NUMTs, their length, homology, and location for potential use in evolutionary studies and to check whether their occurrence causes co-amplification during mitochondrial genome (mtDNA) analyses. Bioinformatic analysis showed 1909 fragments of the mtDNA of D. magna, of which 1630 were located in ten linkage groups (LG) of the nDNA. The best-matched NUMTs covering >90% of the gene sequence have been identified for two mt-tRNA genes, and they may be functional nuclear RNA molecules. Isolating the total DNA in mtDNA studies, co-amplification of nDNA fragments is unlikely in the case of amplification of the whole tRNA genes as well as fragments of other genes. It was observed that TRNA-MET fragments had the highest level of sequence homology, thus they could be evolutionarily the youngest. The lowest homology was found in the D-loop-derived pseudogene. It may probably be the oldest NUMT incorporated into the nDNA; however, further analysis is necessary.
In the Right Place at the Right Time: miRNAs as Key Regulators in Developing Axons
Authors: Corradi Eloina , Baudet Marie-Laure
Published: November 2020

During neuronal circuit formation, axons progressively develop into a presynaptic compartment aided by extracellular signals. Axons display a remarkably high degree of autonomy supported in part by a local translation machinery that permits the subcellular production of proteins required for their development. Here, we review the latest findings showing that microRNAs (miRNAs) are critical regulators of this machinery, orchestrating the spatiotemporal regulation of local translation in response to cues. We first survey the current efforts toward unraveling the axonal miRNA repertoire through miRNA profiling, and we reveal the presence of a putative axonal miRNA signature. We also provide an overview of the molecular underpinnings of miRNA action. Our review of the available experimental evidence delineates two broad paradigms: cue-induced relief of miRNA-mediated inhibition, leading to bursts of protein translation, and cue-induced miRNA activation, which results in reduced protein production. Overall, this review highlights how a decade of intense investigation has led to a new appreciation of miRNAs as key elements of the local translation regulatory network controlling axon development.
Age-Dependent Decline in Synaptic Mitochondrial Function Is Exacerbated in Vulnerable Brain Regions of Female 3xTg-AD Mice
Authors: Espino de la Fuente-Muñoz César , Rosas-Lemus Mónica , Moreno-Castilla Perla , Bermúdez-Rattoni Federico , Uribe-Carvajal Salvador , Arias Clorinda
Published: November 2020

Synaptic aging has been associated with neuronal circuit dysfunction and cognitive decline. Reduced mitochondrial function may be an early event that compromises synaptic integrity and neurotransmission in vulnerable brain regions during physiological and pathological aging. Thus, we aimed to measure mitochondrial function in synapses from three brain regions at two different ages in the 3xTg-AD mouse model and in wild mice. We found that aging is the main factor associated with the decline in synaptic mitochondrial function, particularly in synapses isolated from the cerebellum. Accumulation of toxic compounds, such as tau and Aβ, that occurred in the 3xTg-AD mouse model seemed to participate in the worsening of this decline in the hippocampus. The changes in synaptic bioenergetics were also associated with increased activation of the mitochondrial fission protein Drp1. These results suggest the presence of altered mechanisms of synaptic mitochondrial dynamics and their quality control during aging and in the 3xTg-AD mouse model; they also point to bioenergetic restoration as a useful therapeutic strategy to preserve synaptic function during aging and at the early stages of Alzheimer’s disease (AD).
The Influence of a Stressful Microenvironment on Tumor Exosomes: A Focus on the DNA Cargo
Authors: Domenis Rossana , Cifù Adriana , Curcio Francesco
Published: November 2020

Exosomes secreted by tumor cells, through the transport of bioactive molecules, reprogram the surroundings, building a microenvironment to support the development of the tumor. The discovery that exosomes carry genomic DNA reflecting that of the tumor cell of origin has encouraged studies to use them as non-invasive biomarkers. The exosome-mediated transfer of oncogenes suggested a new mechanism of malignant transformation that could play a role in the formation of metastases. Several studies have examined the role of tumor exosomes on the modulation of the tumor microenvironment, but relatively few have been directed to assess how stressful stimuli can influence their production and cargo. Understanding the changes in exosome loads and the production pattern of the stressed tumor cell may uncover actionable mechanisms responsible for tumor progression.
Mutual Interplay of Host Immune System and Gut Microbiota in the Immunopathology of Atherosclerosis
Authors: Yeh Chih-Fan , Chen Ying-Hsien , Liu Sheng-Fu , Kao Hsien-Li , Wu Ming-Shiang , Yang Kai-Chien , Wu Wei-Kai
Published: November 2020

Inflammation is the key for the initiation and progression of atherosclerosis. Accumulating evidence has revealed that an altered gut microbiome (dysbiosis) triggers both local and systemic inflammation to cause chronic inflammatory diseases, including atherosclerosis. There have been some microbiome-relevant pro-inflammatory mechanisms proposed to link the relationships between dysbiosis and atherosclerosis such as gut permeability disruption, trigger of innate immunity from lipopolysaccharide (LPS), and generation of proatherogenic metabolites, such as trimethylamine N-oxide (TMAO). Meanwhile, immune responses, such as inflammasome activation and cytokine production, could reshape both composition and function of the microbiota. In fact, the immune system delicately modulates the interplay between microbiota and atherogenesis. Recent clinical trials have suggested the potential of immunomodulation as a treatment strategy of atherosclerosis. Here in this review, we present current knowledge regarding to the roles of microbiota in contributing atherosclerotic pathogenesis and highlight translational perspectives by discussing the mutual interplay between microbiota and immune system on atherogenesis.
A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response
Authors: Dumková Jana , Smutná Tereza , Vrlíková Lucie , Dočekal Bohumil , Kristeková Daniela , Večeřa Zbyněk , Husáková Zuzana , Jakešová Veronika , Jedličková Adriena , Mikuška Pavel , Alexa Lukáš , Coufalík Pavel , Tvrdoňová Michaela , Křůmal Kamil , Vaculovič Tomáš , Kanický Viktor , Hampl Aleš , Buchtová Marcela
Published: November 2020

The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)2 NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO3)2 NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO3)2 NP inhalation; conversely, abundance of alpha-smooth muscle actin (α-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor κB (NF-κB) and selected cytokines, such as tumor necrosis factor alpha (TNFα), transforming growth factor beta 1 (TGFβ1), interleukin 6(IL-6), IL-1α and IL-1β , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO3)2 NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period.
Wheat Breeding through Genetic and Physical Mapping
Authors: Gadaleta Agata
Published: November 2020

The Special Issue of “Wheat breeding through genetic and physical mapping” aimed to collect recent advances in research on the genetic and physical mapping of quantitative trait loci (QTLs), candidate genes and regulatory sequences involved in the control of wheat’s important agronomic traits, such as grain yield and quality, biotic and abiotic stress resistance [...]
Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens
Authors: Chlebek Daria , Pinski Artur , Żur Joanna , Michalska Justyna , Hupert-Kocurek Katarzyna
Published: November 2020

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.