bluuebunny/crossref_metadata_2025 huggingface dataset:
Multimodal Intervention to Improve Functional Status in Hypertensive Older Adults: A Pilot Randomized Controlled Trial
Authors: Baptista Liliana C. , Jaeger Byron C. , Anton Stephen D. , Bavry Anthony A. , Handberg Eileen M. , Gardner Anna K. , Harper Sara A. , Roberts Lisa M. , Sandesara Bhanuprasad , Carter Christy S. , Buford Thomas W.
Published: February 2019

This pilot randomized controlled trial (RCT) was designed to provide the preliminary data necessary to conduct a full-scale trial to compare the efficacy of differing first-line antihypertensive medications in improving functional status in older adults, when combined with exercise. The primary objectives were to assess study feasibility, safety, and protocol integrity. Dependent outcomes included gait speed, exercise capacity, body composition, and systemic cardiometabolic biomarkers. Thirty-one physically inactive older adults (70.6 ± 6.1 years) with hypertension and functional limitations were randomly assigned to (1) Perindopril (8 mg/day n = 10), (2) Losartan (100 mg/day; n = 13), or (3) Hydrochlorothiazide (HCTZ: 25 mg/day; n = 8). Participants were also assigned to a 24-week multimodal exercise intervention, separated into an aerobic and concurrent (aerobic + resistance) phase to evaluate potential mode effects. Retention was 84% (26/31), and compliance was >90% and >79% with medication and exercise, respectively. A total of 29 adverse events (Perindopril = 5; Losartan = 12; HCTZ = 11) and one unrelated serious adverse event were observed throughout the trial. Overall, this pilot RCT provided critical data and identified several challenges to ultimately designing and implementing a fully powered trial.
Oil Slick Characterization Using a Statistical Region-Based Classifier Applied to UAVSAR Data
Authors: Genovez Patrícia C. , Jones Cathleen E. , Sant’Anna Sidnei J. S. , Freitas Corina C.
Published: February 2019

During emergency responses to oil spills on the sea surface, quick detection and characterization of an oil slick is essential. The use of Synthetic Aperture Radar (SAR) in general and polarimetric SAR (PolSAR) in particular to detect and discriminate mineral oils from look-alikes is known. However, research exploring its potential to detect oil slick characteristics, e.g., thickness variations, is relatively new. Here a Multi-Source Image Processing System capable of processing optical, SAR and PolSAR data with proper statistical models was tested for the first time for oil slick characterization. An oil seep detected by NASA`s Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) in the Gulf of Mexico was used as a study case. This classifier uses a supervised approach to compare stochastic distances between different statistical distributions (fx) and hypothesis tests to associate confidence levels to the classification results. The classifier was able to detect zoning regions within the slick with high global accuracies and low uncertainties. Two different classes, likely associated with the thicker and thinner oil layers, were recognized. The best results, statistically equivalent, were obtained using different data formats: polarimetric, intensity pair and intensity single-channel. The presence of oceanic features in the form of oceanic fronts and internal waves created convergence zones that defined the shape, spreading and concentration of the thickest layers of oil. The statistical classifier was able to detect the thicker oil layers accumulated along these features. Identification of the relative thickness of spilled oils can increase the oil recovery efficiency, allowing better positioning of barriers and skimmers over the thickest layers. Decision makers can use this information to guide aerial surveillance, in situ oil samples collection and clean-up operations in order to minimize environmental impacts.
Microstructural Evolution of Post-Processed Hastelloy X Alloy Fabricated by Laser Powder Bed Fusion
Authors: Marchese Giulio , Bassini Emilio , Aversa Alberta , Lombardi Mariangela , Ugues Daniele , Fino Paolo , Biamino Sara
Published: February 2019

Hastelloy X (HX) is a Ni-based superalloy which is employed to produce gas turbine and gas-cooled reactor sectors due to its outstanding oxidation resistance and high tensile strength at high temperatures. This alloy can be processed by laser powder bed fusion (LPBF) fabricating complex geometries in a single step. However, post-processing thermal treatments must be applied to generate a suitable microstructure for high-temperature applications. The investigation reports the microstructure evolution of LPBF HX samples under specific post-processing treatments. A hot isostatic pressing (HIP) treatment can close the internal cracks and reduce the residual porosity (less than 0.1%). Moreover, the HIP-triggered recrystallization generated equiaxed grains, while the slow cooling rate generated a film of intergranular carbides (Mo-rich M6C and Cr-rich M23C6) and intragranular carbides (Mo-rich M6C carbides). Therefore, a solution annealing was performed to dissolve the film of carbides which may reduce the ductility. The post solution annealed material consisted of equiaxed grains with ASTM grain size number mainly 4.5-5.5 and inter/intragranular Mo-rich M6C carbides. The microstructure is highly comparable with solution annealed wrought HX alloy. Finally, after simulating short thermal exposure at 745 °C for 6 h, a significant formation of Cr-rich M23C6 carbides was observed strengthening the LPBF HX alloy.
Preliminary Assessment of Criticality Safety Constraints for Swiss Spent Nuclear Fuel Loading in Disposal Canisters
Authors: Vasiliev Alexander , Herrero Jose , Pecchia Marco , Rochman Dimitri , Ferroukhi Hakim , Caruso Stefano
Published: February 2019

This paper presents preliminary criticality safety assessments performed by the Paul Scherrer Institute (PSI) in cooperation with the Swiss National Cooperative for the Disposal of Radioactive Waste (Nagra) for spent nuclear fuel disposal canisters loaded with Swiss Pressurized Water Reactor (PWR) UO2 spent fuel assemblies. The burnup credit application is examined with respect to both existing concepts: taking into account actinides only and taking into account actinides plus fission products. The criticality safety calculations are integrated with uncertainty quantifications that are as detailed as possible, accounting for the uncertainties in the nuclear data used, fuel assembly and disposal canister design parameters and operating conditions, as well as the radiation-induced changes in the fuel assembly geometry. Furthermore, the most penalising axial and radial burnup profiles and the most reactive fuel loading configuration for the canisters were taken into account accordingly. The results of the study are presented with the help of loading curves showing what minimum average fuel assembly burnup is required for the given initial fuel enrichment of fresh fuel assemblies to ensure that the effective neutron multiplication factor, k e f f , of the canister would comply with the imposed criticality safety criterion.
Molecular Probes for Evaluation of Oxidative Stress by In Vivo EPR Spectroscopy and Imaging: State-of-the-Art and Limitations
Authors: Babić Nikola , Peyrot Fabienne
Published: February 2019

Oxidative stress, defined as a misbalance between the production of reactive oxygen species and the antioxidant defenses of the cell, appears as a critical factor either in the onset or in the etiology of many pathological conditions. Several methods of detection exist. However, they usually rely on ex vivo evaluation or reports on the status of living tissues only up to a few millimeters in depth, while a whole-body, real-time, non-invasive monitoring technique is required for early diagnosis or as an aid to therapy (to monitor the action of a drug). Methods based on electron paramagnetic resonance (EPR), in association with molecular probes based on aminoxyl radicals (nitroxides) or hydroxylamines especially, have emerged as very promising to meet these standards. The principles involve monitoring the rate of decrease or increase of the EPR signal in vivo after injection of the nitroxide or the hydroxylamine probe, respectively, in a pathological versus a control situation. There have been many successful applications in various rodent models. However, current limitations lie in both the field of the technical development of the spectrometers and the molecular probes. The scope of this review will mainly focus on the latter.
Desiderata for Fractional Derivatives and Integrals
Authors: Hilfer Rudolf , Luchko Yuri
Published: February 2019

The purpose of this brief article is to initiate discussions in this special issue by proposing desiderata for calling an operator a fractional derivative or a fractional integral. Our desiderata are neither axioms nor do they define fractional derivatives or integrals uniquely. Instead they intend to stimulate the field by providing guidelines based on a small number of time honoured and well established criteria.
New Acyclic Cytotoxic Jasplakinolide Derivative from the Marine Sponge Jaspis splendens
Authors: Ebada Sherif S. , Müller Werner E. G. , Lin Wenhan , Proksch Peter
Published: February 2019

A new acylic jasplakinolide congener (2), another acyclic derivative requiring revision (4), together with two jasplakinolide derivatives including the parent compound jasplakinolide (1) were isolated from the Indonesian marine sponge Jaspis splendens. The chemical structures of the new and known compounds were unambiguously elucidated based on HRESIMS and exhaustive 1D and 2D NMR spectral analysis as well as a comparison of their NMR data with those of jasplakinolide (1). The isolated jasplakinolides inhibited the growth of mouse lymphoma (L5178Y) cells in vitro with IC50 values in the low micromolar to nanomolar range.
Isolation and Structure-Activity Relationship of Subergorgic Acid and Synthesis of Its Derivatives as Antifouling Agent
Authors: Zhang Jun , Ling Wei , Yang Zhiqiang , Liang Yan , Zhang Linyan , Guo Can , Wang Kailing , Zhong Balian , Xu Shihai , Xu Ying
Published: February 2019

In this study, as part of our continuous search for environmentally-friendly antifoulants from natural resources, subergorgic acid (SA) was identified from the gorgonian coral Subergorgia suberosa, demonstrating non-toxic, significant inhibitory effects (EC50 1.25 μg/mL, LC50 > 25 μg/mL) against the settlement of Balanus amphitrite. To further explore the bioactive functional groups of SA and synthesize more potent antifouling compounds based on the lead SA, the structure-activity relationships of SA were studied, followed by rational design and synthesis of two series of SA derivatives (one being benzyl esters of SA and another being SA derivatives containing methylene chains of various lengths). Our results indicated that (1) both the double bond and ketone carbonyl are essential elements responsible for the antifouling effect of SA, while the acid group is not absolutely necessary for maintaining the antifouling effect; (2) all benzyl esters of SA displayed good antifouling effects (EC50 ranged from 0.30 to 2.50 μg/mL) with the most potent compound being 5 (EC50 0.30 μg/mL, LC50 > 25 μg/mL), which was over four-fold more potent than SA; and (3) the introduction of a methylene chain into SA reduces the antifouling potency while the length of the methylene chain may differently influence the antifouling effect, depending on the functional group at the opposite site of the methylene chain. Not only has this study successfully revealed the bioactive functional groups of SA, contributing to the mechanism of SA against the settlement of B. amphitrite, but it has also resulted in the identification of a more potent compound 5, which might represent a non-toxic, high-efficiency antifoulant.
Eosinophilic Upper Airway Inflammation in a Murine Model Using an Adoptive Transfer System Induces Hyposmia and Epithelial Layer Injury with Convex Lesions
Authors: Kanda Akira , Kondo Kenji , Hosaka Naoki , Kobayashi Yoshiki , Bui Dan Van , Yun Yasutaka , Suzuki Kensuke , Sawada Shunsuke , Asako Mikiya , Nakamura Akihiko , Tomoda Koichi , Sakata Yoshiko , Tsuta Koji , Dombrowicz David , Kawauchi Hideyuki , Fujieda Shigeharu , Iwai Hiroshi
Published: February 2019

Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a refractory upper airway disease, accompanied mainly by eosinophilia and/or asthma. In addition, the disease correlates with a high rate of hyposmia, following a marked infiltration of eosinophils into the inflamed site, the paranasal sinus. Although eosinophils are known to contribute to the development of hyposmia and CRSwNP pathology, the underlying mechanisms remain unclear. This study aimed to investigate whether eosinophilic upper airway inflammation induces hyposmia and CRSwNP in a murine model using an adoptive transfer system. Methods: To induce eosinophilic rhinosinusitis, splenocytes, including a high proportion (over 50%) of activated eosinophils (SPLhEos), were collected from interleukin-5 transgenic mice following double intraperitoneal injections of antigens, such as ovalbumin, house dust mite, or fungus. Activated SPLhEos with corresponding antigens were then transferred into the nasal cavity of recipient mice, which were sensitized and challenged by the corresponding antigen four times per week. Olfactory function, histopathological, and computed tomography (CT) analyses were performed 2 days after the final transfer of eosinophils. Results: Hyposmia was induced significantly in mice that received SPLhEos transfer compared with healthy and allergic mice, but it did not promote morphological alteration of the paranasal sinus. Pathological analysis revealed that epithelial layer injury and metaplasia similar to polyps, with prominent eosinophil infiltration, was induced in recipient tissue. However, there was no nasal polyp development with interstitial edema that was similar to those recognized in human chronic rhinosinusitis. Conclusions: This study supports the previously unsuspected contribution of eosinophils to CRS development in the murine model and suggests that murine-activated eosinophilic splenocytes contribute to the development of hyposmia due to more mucosal inflammation than physical airway obstruction and epithelial layer injury with convex lesions.
24th International Symposium on Infections in the Critically Ill Patient
Authors: Artigas Antonio , Carlet Jean , Garnacho José , Niederman Michael , Torres Antoni
Published: February 2019

This 24th International Symposium on Infections in the Critically Ill  [...]
Transglutaminase 2: The Maestro of the Oncogenic Mediators in Renal Cell Carcinoma
Authors: Nezir Ayca Ece , Ulukan Burge , Telci Dilek
Published: February 2019

Transglutaminase 2 (TG2) is a multifunctional crosslinking enzyme that displays transamidation, protein disulfide isomerase, protein kinase, as well as GTPase and ATPase activities. TG2 can also act as an adhesion molecule involved in the syndecan and integrin receptor signaling. In recent years, TG2 was implicated in cancer progression, survival, invasion, migration, and stemness of many cancer types, including renal cell carcinoma (RCC). Von Hippel-Lindau mutations leading to the subsequent activation of Hypoxia Inducible Factor (HIF)-1-mediated signaling pathways, survival signaling via the PI3K/Akt pathway resulting in Epithelial Mesenchymal Transition (EMT) metastasis and angiogenesis are the main factors in RCC progression. A number of studies have shown that TG2 was important in HIF-1- and PI3K-mediated signaling, VHL and p53 stabilization, glycolytic metabolism and migratory phenotype in RCC. This review focuses on the role of TG2 in the regulation of molecular pathways nurturing not only the development and propagation of RCC, but also drug-resistance and metastatic potential.
Treatment of Two-Phase Olive Mill Wastewater and Recovery of Phenolic Compounds Using Membrane Technology
Authors: Sygouni Varvara , Pantziaros Alexis G. , Iakovides Iakovos C. , Sfetsa Evangelia , Bogdou Polychronia I. , Christoforou Emilia A. , Paraskeva Christakis A.
Published: February 2019

The semi-solid wastes (pomace or alperujo) produced in the two-phase olive oil extraction process contains extremely high organic load and phenolic substances. Efficient treatment of such kinds of wastes using membrane filtration, should be sought to reduce the hazardous effects to the environment. On the other hand, phenolic compounds can be isolated and purified up to a level of commercial exploitation using the membrane technology. Firstly, the extraction process with mixtures of water and ethanol was optimized by testing extraction parameters (e.g., solvent’s mixture, duration, and temperature) at laboratory scale. Next, extraction was conducted using larger volumes and the treatment was continued in a pilot membrane filtration system, consisted of ultrafiltration (UF), nanofiltration (NF) and reverse osmosis (RO) membranes. The extracted solution from the olive oil pomace was fed to the pilot membrane filtration system, where all fat, lipids, and solids were removed while the phenolic compounds were concentrated in the retentate streams of NF and/or RO. Total phenolic content (TPC) at the RO’s concentrate stream was 225 mg/L and at the final effluent was lower than 10 mg/lt. The chemical oxygen demand (COD) value at the final effluent was much lower (~280 mg/L) than in the feed stream (>32,000 mg/L).
Direct Correlations among the Grain Size, Texture, and Indentation Behavior of Nanocrystalline Nickel Coatings
Authors: Feng Lu , Ren Yong-Yue , Zhang Yan-Heng , Wang Shibin , Li Linan
Published: February 2019

By using nc-Ni coatings as a model system, systematic experiments were designed to evaluate the interaction between the microstructural and mechanical properties tailored by electrodeposition conditions. A direct correlation between grain size and texture was established for the first time. The grain size of the (111) crystalline plane decreases with the texture coefficient (RTC) regardless of the process conditions, and that of the (220) plane has different trends. Then, a peculiar phenomenon is revealed that the dependence of hardness on grain size is accurately described by the Hall-Petch relationship when changing the temperature or pH, but with different slopes, while it deviates from such a relationship with changing current density, denoting more underlying mechanisms related to texture. Finally, a surprising degree of influence of texture on hardness and elastic modulus is also presented, with the overall trend of hardness increasing with texture; and when the RTC of (111) exceeds 40%, the elastic modulus increases with texture, implying a fundamental relationship between modulus and texture. Texture predominates over the other factors on the elastic modulus, revealing the importance of elastic anisotropy. Significantly, the present work suggests a useful tailoring routine to fabricate high quality nc-Ni coatings with the desired structure and mechanical properties.
Leaching Chalcopyrite Concentrate with Oxygen and Sulfuric Acid Using a Low-Pressure Reactor
Authors: Cháidez Josué , Parga José , Valenzuela Jesús , Carrillo Raúl , Almaguer Isaías
Published: February 2019

This article presents a copper leaching process from chalcopyrite concentrates using a low-pressure reactor. The experiments were carried out in a 30 L batch reactor at an oxygen pressure of 1 kg/cm2 and solid concentration of 100 g/L. The temperature, particle size and initial acid concentration were varied based on a Taguchi L9 experimental design. The initial and final samples of the study were characterized by chemical analysis, X-ray diffraction and particle size distribution. The mass balance showed that 98% of copper was extracted from the chalcopyrite concentrate in 3 h under the following experimental conditions: 130 g/L of initial sulfuric acid concentration, temperature of 100 °C, oxygen pressure of 1 kg/cm2, solid concentration of 100 g/L and particle size of −105 + 75 μm. The ANOVA demonstrated that temperature had the greatest influence on copper extraction. The activation energy was 61.93 kJ/mol. The best fit to a linear correlation was the chemical reaction equation that controls the kinetics for the leaching copper from chalcopyrite. The images obtained by SEM showed evidence of shrinking in the core model with the formation of a porous elemental sulfur product layer.
Fluid Flow Turbulence in the Proximities of the Metal-Slag Interface in Ladle Stirring Operations
Authors: Calderón-Hurtado Fabian Andrés , Morales Dávila Rodolfo , Chattopadhyay Kinnor , García-Hernández Saúl
Published: February 2019

Three-phase interactions (metal-slag-argon) in ladle stirring operations have strong effects on the metal-slag mass transfer processes. Specifically, the thickness of the slag controls the fluid turbulence to an extent that once trespassing a critical thickness, increases of stirring strength no longer effect the flow. To analyze these conditions, a physical model considering the three phases was built to study liquid turbulence in the proximities of the metal-slag interface. A velocity probe placed close to the interface permitted the continuous monitoring and statistical analyses of any turbulence. The slag eye opening was found to be strongly dependent on the stirring conditions, and the mixing times decreased with thin slag thicknesses. Slag entrainment was enhanced with thick slag layers and high flow rates of the gas phase. A multiphase model was developed to simulate these results and was found to be a good agreement between experimental and numerical results.
Current Developments in µMAS NMR Analysis for Metabolomics
Authors: Lucas-Torres Covadonga , Wong Alan
Published: February 2019

Analysis of microscopic specimens has emerged as a useful analytical application in metabolomics because of its capacity for characterizing a highly homogenous sample with a specific interest. The undeviating analysis helps to unfold the hidden activities in a bulk specimen and contributes to the understanding of the fundamental metabolisms in life. In NMR spectroscopy, micro(µ)-probe technology is well-established and -adopted to the microscopic level of biofluids. However, this is quite the contrary with specimens such as tissue, cell and organism. This is due to the substantial difficulty of developing a sufficient µ-size magic-angle spinning (MAS) probe for sub-milligram specimens with the capability of high-quality data acquisition. It was not until 2012; a µMAS probe had emerged and shown promises to µg analysis; since, a continuous advancement has been made striving for the possibility of µMAS to be an effective NMR spectroscopic analysis. Herein, the mini-review highlights the progress of µMAS development—from an impossible scenario to an attainable solution—and describes a few demonstrative metabolic profiling studies. The review will also discuss the current challenges in µMAS NMR analysis and its potential to metabolomics.
Towards an Ultra-Sensitive Temperature Sensor for Uncooled Infrared Sensing in CMOS–MEMS Technology
Authors: Göktaş Hasan
Published: February 2019

Microbolometers and photon detectors are two main technologies to address the needs in Infrared Sensing applications. While the microbolometers in both complementary metal-oxide semiconductor (CMOS) and Micro-Electro-Mechanical Systems (MEMS) technology offer many advantages over photon detectors, they still suffer from nonlinearity and relatively low temperature sensitivity. This paper not only offers a reliable solution to solve the nonlinearity problem but also demonstrate a noticeable potential to build ultra-sensitive CMOS–MEMS temperature sensor for infrared (IR) sensing applications. The possibility of a 31× improvement in the total absolute frequency shift with respect to ambient temperature change is verified via both COMSOL (multiphysics solver) and theory. Nonlinearity problem is resolved by an operating temperature sensor around the beam bending point. The effect of both pull-in force and dimensional change is analyzed in depth, and a drastic increase in performance is achieved when the applied pull-in force between adjacent beams is kept as small as possible. The optimum structure is derived with a length of 57 µm and a thickness of 1 µm while avoiding critical temperature and, consequently, device failure. Moreover, a good match between theory and COMSOL is demonstrated, and this can be used as a guidance to build state-of-the-art designs.
Phosphonium-based Ionic Liquid Modified Activated Carbon from Mixed Recyclable Waste for Mercury(II) Uptake
Authors: Habila Mohamed A. , AlOthman Zeid A. , Ghfar Ayman A. , Al-Zaben Maha I.M. , Alothman Ahmed A.S. , Abdeltawab Ahmed A. , El-Marghany Adel , Sheikh Mohamed
Published: February 2019

The contamination of water surfaces by mercury is a dangerous environmental problem due to its toxicity, which leads kidney damage. Activated carbon from mixed recyclable waste modified by phosphonium-based ionic liquid (IL-ACMRW) was therefore prepared and evaluated for Hg(II) remediation. The activated carbon used in this study was prepared from mixed waste, including cardboard, papers and palm wastes as cheap raw materials. The mixed Recyclable Waste Activated Carbon was combined with trihexyl(tetradecyl)phosphonium Bis2,4,4-(trimethylpentyl)phosphinate (Cyphos® IL 104) ionic liquid to form an adsorbent with organic-inorganic content, in order to improve the Hg(II) uptake from aqueous solutions. FTIR confirms the presence of P, C=O and OH after this modification. The adsorption process was investigated and the evaluated results showed that the capacity was 124 mg/g at pH 4, with a contact time of 90 min, an adsorbent dose of 0.4 g/L, and a Hg(II) concentration of 50 mg/L. This Hg(II) adsorption capacity is superior than that reported in the literature for modified multiwall carbon nanotubes. The adsorption of Hg(II) on the modified activated carbon from mixed recyclable waste was found to follow the pseudo second-order kinetics model. Isotherms of adsorption were analyzed via Freundlich and Langmuir models. The results indicated that Freundlich is the best model to describe the process, suggesting multilayer adsorption.
Fluorescent DNA Biosensor for Single-Base Mismatch Detection Assisted by Cationic Comb-Type Copolymer
Authors: Han Jialun , Wu Jincai , Du Jie
Published: February 2019

Simple and rapid detection of DNA single base mismatch or point mutation is of great significance for the diagnosis, treatment, and detection of single nucleotide polymorphism (SNP) in genetic diseases. Homogeneous mutation assays with fast hybridization kinetics and amplified discrimination signals facilitate the automatic detection. Herein we report a quick and cost-effective assay for SNP analysis with a fluorescent single-labeled DNA probe. This convenient strategy is based on the efficient quenching effect and the preferential binding of graphene oxide (GO) to ssDNA over dsDNA. Further, a cationic comb-type copolymer (CCC), poly(l-lysine)-graft-dextran (PLL-g-Dex), significantly accelerates DNA hybridization and strand-exchange reaction, amplifying the effective distinction of the kinetic barrier between a perfect matched DNA and a mismatched DNA. Moreover, in vitro experiments indicate that RAW 264.7 cells cultured on PLL-g-Dex exhibits excellent survival and proliferation ability, which makes this mismatch detection strategy highly sensitive and practical.
Elucidation of the Mechanism of Action for Metal Based Anticancer Drugs by Mass Spectrometry-Based Quantitative Proteomics
Authors: Jia Shuailong , Wang Runjing , Wu Kui , Jiang Hongliang , Du Zhifeng
Published: February 2019

The discovery of the anticancer activity of cisplatin and its clinical application has opened a new field for studying metal-coordinated anticancer drugs. Metal-based anticancer drugs, such as cisplatin, can be transported to cells after entering into the human body and form metal–DNA or metal–protein adducts. Then, responding proteins will recognize adducts and form stable complexes. The proteins that were binding with metal-based anticancer drugs were relevant to their mechanism of action. Herein, investigation of the recognition between metal-based anticancer drugs and its binding partners will further our understanding about the pharmacology of cytotoxic anticancer drugs and help optimize the structure of anticancer drugs. The “soft” ionization mass spectrometric methods have many advantages such as high sensitivity and low sample consumption, which are suitable for the analyses of complex biological samples. Thus, MS has become a powerful tool for the identification of proteins binding or responding to metal-based anticancer drugs. In this review, we focused on the mass spectrometry-based quantitative strategy for the identification of proteins specifically responding or binding to metal-based anticancer drugs, ultimately elucidating their mechanism of action.
Improved Efficiency of Perovskite Solar Cells by the Interfacial Modification of the Active Layer
Authors: Lu Zhen , Wang Shangzhi , Liu Huijun , Feng Feng , Li Wenhua
Published: February 2019

As the most promising material for thin-film solar cells nowadays, perovskite shine for its unique optical and electronic properties. Perovskite-based solar cells have already been demonstrated with high efficiencies. However, it is still very challenging to optimize the morphology of perovskite film. In this paper we proposed a smooth and continuous perovskite active layer by treating the poly (3, 4-ethylenedioxythiophene): poly (styrenesulphonate) (PEDOT:PSS) with pre-perovskite deposition and dimethylsulfoxide (DMSO) rinse. The scanning electron microscope (SEM) and atomic force microscope (AFM) images confirmed a perovskite active layer consisting of large crystal grains with less grain boundary area and enhanced crystallinity. The perovskite devices fabricated by this method feature a high power conversion efficiency (PCE) of 11.36% and a short-circuit current (Jsc) of 21.9 mA·cm−2.
A Microwave-Assisted Synthesis of Zinc Oxide Nanocrystals Finely Tuned for Biological Applications
Authors: Garino Nadia , Limongi Tania , Dumontel Bianca , Canta Marta , Racca Luisa , Laurenti Marco , Castellino Micaela , Casu Alberto , Falqui Andrea , Cauda Valentina
Published: February 2019

Herein we report a novel, easy, fast and reliable microwave-assisted synthesis procedure for the preparation of colloidal zinc oxide nanocrystals (ZnO NCs) optimized for biological applications. ZnO NCs are also prepared by a conventional solvo-thermal approach and the properties of the two families of NCs are compared and discussed. All of the NCs are fully characterized in terms of morphological analysis, crystalline structure, chemical composition and optical properties, both as pristine nanomaterials or after amino-propyl group functionalization. Compared to the conventional approach, the novel microwave-derived ZnO NCs demonstrate outstanding colloidal stability in ethanol and water with long shelf-life. Furthermore, together with their more uniform size, shape and chemical surface properties, this long-term colloidal stability also contributes to the highly reproducible data in terms of biocompatibility. Actually, a significantly different biological behavior of the microwave-synthesized ZnO NCs is reported with respect to NCs prepared by the conventional synthesis procedure. In particular, consistent cytotoxicity and highly reproducible cell uptake toward KB cancer cells are measured with the use of microwave-synthesized ZnO NCs, in contrast to the non-reproducible and scattered data obtained with the conventionally-synthesized ones. Thus, we demonstrate how the synthetic route and, as a consequence, the control over all the nanomaterial properties are prominent points to be considered when dealing with the biological world for the achievement of reproducible and reliable results, and how the use of commercially-available and under-characterized nanomaterials should be discouraged in this view.
In-Situ Fabrication of g-C3N4/ZnO Nanocomposites for Photocatalytic Degradation of Methylene Blue: Synthesis Procedure Does Matter
Authors: Zhang Shengqiang , Su Changsheng , Ren Hang , Li Mengli , Zhu Longfeng , Ge Shuang , Wang Min , Zhang Zulei , Li Lei , Cao Xuebo
Published: February 2019

The nanocomposite preparation procedure plays an important role in achieving a well-established heterostructured junction, and hence, an optimized photocatalytic activity. In this study, a series of g-C3N4/ZnO nanocomposites were prepared through two distinct procedures of a low-cost, environmentally-friendly, in-situ fabrication process, with urea and zinc acetate being the only precursor materials. The physicochemical properties of synthesized g-C3N4/ZnO composites were mainly characterized by XRD, UV–VIS diffuse reflectance spectroscopy (DRS), N2 adsorption-desorption, FTIR, TEM, and SEM. These nanocomposites’ photocatalytic properties were evaluated in methylene blue (MB) dye photodecomposition under UV and sunlight irradiation. Interestingly, compared with ZnO nanorods, g-C3N4/ZnO nanocomposites (x:1, obtained from urea and ZnO nanorods) exhibited weak photocatalytic activity likely due to a “shading effect”, while nanocomposites (x:1 CN, made from g-C3N4 and zinc acetate) showed enhanced photocatalytic activity that can be ascribed to the effective establishment of heterojunctions. A kinetics study showed that a maximum reaction rate constant of 0.1862 min-1 can be achieved under solar light illumination, which is two times higher than that of bare ZnO nanorods. The photocatalytic mechanism was revealed by determining reactive species through adding a series of scavengers. It suggested that reactive ●O2− and h+ radicals played a major role in promoting dye photodegradation.
Structural and Optical Characteristics of PVA:C-Dot Composites: Tuning the Absorption of Ultra Violet (UV) Region
Authors: Aziz Shujahadeen B. , Hassan Aso Q. , Mohammed Sewara J. , Karim Wrya O. , F. Z. Kadir M. , A. Tajuddin H. , N. M. Y. Chan N.
Published: February 2019

In this work the influence of carbon nano-dots (CNDs) on absorption of ultra violet (UV) spectra in hybrid PVA based composites was studied. The FTIR results reveal the complex formation between PVA and CNDs. The shifting was observed in XRD spectrum of PVA:CNDs composites compared to pure PVA. The Debye-Scherrer formula was used to calculate the crystallite size of CNDs and crystalline phases of pure PVA and PVA:CNDs composites. The FESEM images emphasized the presence and dispersion of C-dots on the surface of the composite samples. From the images, a strong and clear absorption was noticed in the spectra. The strong absorption that appeared peaks at 280 nm and 430 nm can be ascribed to the n-π* and π-π* transitions, respectively. The absorption edge shifted to lower photon energy sides with increasing CNDs. The luminescence behavior of PVA:CNDs composite was confirmed using digital and photo luminescence (PL) measurements. The optical dielectric constant which is related to the density of states was studied and the optical band gap was characterized accurately using optical dielectric loss parameter. The Taucs model was used to determine the type of electronic transition in the samples.
The Thermal, Electrical and Thermoelectric Properties of Graphene Nanomaterials
Authors: Wang Jingang , Mu Xijiao , Sun Mengtao
Published: February 2019

Graphene, as a typical two-dimensional nanometer material, has shown its unique application potential in electrical characteristics, thermal properties, and thermoelectric properties by virtue of its novel electronic structure. The field of traditional material modification mainly changes or enhances certain properties of materials by mixing a variety of materials (to form a heterostructure) and doping. For graphene as well, this paper specifically discusses the use of traditional modification methods to improve graphene’s electrical and thermoelectrical properties. More deeply, since graphene is an atomic-level thin film material, its shape and edge conformation (zigzag boundary and armchair boundary) have a great impact on performance. Therefore, this paper reviews the graphene modification field in recent years. Through the change in the shape of graphene, the change in the boundary structure configuration, the doping of other atoms, and the formation of a heterostructure, the electrical, thermal, and thermoelectric properties of graphene change, resulting in broader applications in more fields. Through studies of graphene’s electrical, thermal, and thermoelectric properties in recent years, progress has been made not only in experimental testing, but also in theoretical calculation. These aspects of graphene are reviewed in this paper.
Acute Effects of Hibiscus sabdariffa Calyces on Postprandial Blood Pressure, Vascular Function, Blood Lipids, Biomarkers of Insulin Resistance and Inflammation in Humans
Authors: Abubakar Salisu M. , Ukeyima Moses T. , Spencer Jeremy P. E. , Lovegrove Julie A.
Published: February 2019

Background/Objectives: The acute impact of Hibiscus sabdariffa calyces (HSC) extract on postprandial vascular function and other cardiometabolic risk factors have not been studied previously. This study investigated the acute impact of HSC extract consumption on blood pressure (BP), vascular function and other cardiometabolic risk markers. Subjects/Methods: Twenty-five men with 1% to 10% cardiovascular disease (CVD) risk (determined by QRISK®2) were randomised to consume either 250 mL of the aqueous extract of HSC or water with breakfast in a randomised, controlled, single-blinded, 2-meal cross-over study (ClinicalTrials.gov, NTC02165553) with a two weeks washout period between study days. BP was measured at baseline and hourly for 4 h. Flow mediated dilatation (FMD) of the branchial artery was measured at baseline, 2 and 4 h post intervention drink consumption. Results: Acute consumption of aqueous extract of HSC caused a significant increase in % FMD (p < 0.001), a non-significant decrease in systolic BP (SBP) and diastolic BP (DBP); non-significant increase in urinary and plasma nitric oxide (NOx) and reduced response of serum glucose, plasma insulin, serum triacylglycerol and C-reactive protein (CRP) levels; significant (p = 0.026) improvement in the area under systemic antioxidant response curve (0 to 2 h); no significant changes in arterial stiffness following the acute consumption of the extract of HSC. Gallic acid, 4-O-methylgallic acid, 3-O-methylgallic acid and hippuric acid reached a maximum plasma concentration at 1 to 2 h post consumption of the extract of HSC. Conclusion: The extract of HSC improved postprandial vascular function and may be a useful dietary strategy to reduce endothelial dysfunction and CVD risk, although this requires confirmation.
Vitamin D Supplementation and Physical Activity of Young Soccer Players during High-Intensity Training
Authors: Skalska Maria , Nikolaidis Pantelis Theo , Knechtle Beat , Rosemann Thomas Johannes , Radzimiński Łukasz , Jastrzębska Joanna , Kaczmarczyk Mariusz , Myśliwiec Artur , Dragos Paul , López-Sánchez Guillermo F. , Jastrzębski Zbigniew
Published: February 2019

The aim of this study was to confirm that vitamin D supplementation of young soccer players during eight-week high-intensity training would have a significant effect on their motion activity. The subjects were divided into two groups: the experimental one, which was supplemented with vitamin D (SG, n = 20), and the placebo group (PG, n = 16), which was not supplemented with vitamin D. All the players were subjected to the same soccer training, described as High-Intensity Interval Training (HIIT). The data of the vitamin D status, time motion parameters and heart rate were collected just before and after the intervention. A significant increase in 25(OH)D concentration (119%) was observed in the supplemented group, while the non-supplemented group showed a decrease of 8.4%. Based on the obtained results, it was found that physical activity indicators in the players were significantly improved during small-sided games at the last stage of the experiment. However, taking into account the effect of supplementation with vitamin D, there were no statistically significant differences between the placebo and the supplemented groups; thus, the effect size of the conducted experiment was trivial.
Comparative Study of Two Oxidizing Agents, Chloramine T and Iodo-Gen®, for the Radiolabeling of β-CIT with Iodine-131: Relevance for Parkinson’s Disease
Authors: R. Durante Ana Claudia , V. Sobral Danielle , C. Miranda Ana Claudia , V. de Almeida Érika , L. Fuscaldi Leonardo , R. F. F. de Barboza Marycel , Malavolta Luciana
Published: February 2019

Parkinson’s disease (PD) is a neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta, leading to alteration of the integrity of dopaminergic transporters (DATs). In recent years, some radiopharmaceuticals have been used in the clinic to evaluate the integrity of DATs. These include tropane derivatives such as radiolabeled β-CIT and FP-CIT with iodine-123 (123I), and TRODAT-1 with metastable technetium-99 (99mTc). Radiolabeling of β-CIT with radioactive iodine is based on electrophilic radioiodination using oxidizing agents, such as Chloramine T or Iodo-Gen®. For the first time, the present work performed a comparative study of the radiolabeling of β-CIT with iodine-131 (131I), using either Chloramine T or Iodo-Gen® as oxidizing agents, in order to improve the radiolabeling process of β-CIT and to choose the most advantageous oxidizing agent to be used in nuclear medicine. Both radiolabeling methods were similar and resulted in high radiochemical yield (> 95%), with suitable 131I-β-CIT stability up to 72 h. Although Chloramine T is a strong oxidizing agent, it was as effective as Iodo-Gen® for β-CIT radiolabeling with 131I, with the advantage of briefer reaction time and solubility in aqueous medium.
Optimal Operating Schedule for Energy Storage System: Focusing on Efficient Energy Management for Microgrid
Authors: Jung Sooyoung , Yoon Yong Tae
Published: February 2019

A microgrid is a group of many small-scale distributed energy resources, such as solar/wind energy sources, diesel generators, energy storage units, and electric loads. As a small-scale power grid, it can be operated independently or within an existing power grid(s). The microgrid energy management system is a system that controls these components to achieve optimized operation in terms of price by reducing costs and maximizing efficiency in energy consumption. A post-Industry-4.0 consumer requires an optimal design and control of energy storage based on a demand forecast, using big data to stably supply clean, new, and renewable energy when necessary while maintaining a consistent level of quality. Thus, this study focused on software technology through which an optimized operation schedule for energy storage in a microgrid is derived. This energy storage operation schedule minimizes the costs involved in electricity use. For this, an optimization technique is used that sets an objective function representing the information and costs pertaining to electricity use, while minimizing its value by using Mixed Integer Linear Programming or a Genetic Algorithm. The main feature of the software is that an optimal operation schedule derivation function has been implemented with MATLAB for the following circumstances: when the basic operation rules are applied, when operating with another grid, when the external operating conditions are applied, and when the internal operating conditions are applied.
Reflections on the Evolution of the State of the Art
Authors: Levine Daniel
Published: February 2019

Reflections on the evolution of the state of the art in the study of religion, society, and politics in Latin America over the last five decades begin with a critical assessment of the conventional wisdom of fifty years ago, as conveyed in texts and in graduate education. Stress was placed on modernization and secularization (with religion depicted as static and destined to decline) on consensus as a foundation for social life, and on drawing clear lines between religion and politics. These concepts were of little use when confronted in the late 1960s with a reality of continuous change, conflict, and efforts from left and right to assert a public role for religion. Working concepts of religion and politics had to be broadened well beyond church and state. Conceptual space had to be found for religious pluralism as the emergence of Pentecostal and evangelical churches was putting an end to centuries of Catholic monopoly: Latin America was becoming religiously plural. The state of the art is now much improved. Current and future research could usefully focus attention on issues like sexuality, gender, and identity, spirituality and encounters with charismatic power, and the new realities of religion and violence. Mid-range theories that give prominence to change and to the relation among social levels, and mixed methodologies that highlight meaning and significance will be central to any future state of the art that can make sense of a reality marked by continuing waves of creative change.
Devising Mineral Resource Supply Pathways to a Low-Carbon Electricity Generation by 2100
Authors: Boubault Antoine , Maïzi Nadia
Published: February 2019

Achieving a “carbon neutral” world by 2100 or earlier in a context of economic growth implies a drastic and profound transformation of the way energy is supplied and consumed in our societies. In this paper, we use life-cycle inventories of electricity-generating technologies and an integrated assessment model (TIMES Integrated Assessment Model) to project the global raw material requirements in two scenarios: a second shared socioeconomic pathway baseline, and a 2 °C scenario by 2100. Material usage reported in the life-cycle inventories is distributed into three phases, namely construction, operation, and decommissioning. Material supply dynamics and the impact of the 2 °C warming limit are quantified for three raw fossil fuels and forty-eight metallic and nonmetallic mineral resources. Depending on the time horizon, graphite, sand, sulfur, borates, aluminum, chromium, nickel, silver, gold, rare earth elements or their substitutes could face a sharp increase in usage as a result of a massive installation of low-carbon technologies. Ignoring nonfuel resource availability and value in deep decarbonation, circular economy, or decoupling scenarios can potentially generate misleading, contradictory, or unachievable climate policies.
A Systematic Review of Perception System and Simulators for Autonomous Vehicles Research
Authors: Rosique Francisca , Navarro Pedro J. , Fernández Carlos , Padilla Antonio
Published: February 2019

This paper presents a systematic review of the perception systems and simulators for autonomous vehicles (AV). This work has been divided into three parts. In the first part, perception systems are categorized as environment perception systems and positioning estimation systems. The paper presents the physical fundamentals, principle functioning, and electromagnetic spectrum used to operate the most common sensors used in perception systems (ultrasonic, RADAR, LiDAR, cameras, IMU, GNSS, RTK, etc.). Furthermore, their strengths and weaknesses are shown, and the quantification of their features using spider charts will allow proper selection of different sensors depending on 11 features. In the second part, the main elements to be taken into account in the simulation of a perception system of an AV are presented. For this purpose, the paper describes simulators for model-based development, the main game engines that can be used for simulation, simulators from the robotics field, and lastly simulators used specifically for AV. Finally, the current state of regulations that are being applied in different countries around the world on issues concerning the implementation of autonomous vehicles is presented.