bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Classroom Culture and Psychological Safety in EFL Students' Engagement
Authors: Tu Xiaowei
Published: September 2021

Language learning is a complex phenomenon that is the outcome of an interplay of numerous inter/intra-personal variables. Out of these factors, emotions play a critical role in the whole process of learning. Research approves that positive emotions lead to positive outcomes. This is only obtainable in a positive classroom culture where students feel psychologically safe. If so, they actively engage in the classroom activities for a longer period. However, the macro-effect of classroom culture in EFL/ESL contexts has been limitedly explored. Against this shortcoming, the present article provides a brief account of the definition and conceptualization of classroom culture and its impact on two learner psychology variables (i.e., psychological safety, engagement). Moreover, the dimensions and factors influencing these variables are discussed. Finally, the study offers some implications for different stakeholders in EFL/ESL contexts and enumerates a number of research gaps and future directions for future scholars in this line of inquiry.
Examining Mental Health Differences Between Transgender, Gender Nonconforming, and Cisgender Young People in British Columbia
Authors: Pattison Rachal , Puyat Joseph H. , Giesbrecht Allison , Zenone Marco , Mathias Steve , Barbic Skye
Published: September 2021

Foundry is an integrated service network delivering services to young people across British Columbia, Canada. To better understand the needs of transgender and gender nonconforming young people accessing Foundry—this study compares rates of mental health distress between transgender and gender nonconforming young people and cisgender young people accessing services and examines the extent to which race may have amplified the association between transgender and gender nonconforming identity and mental health distress. We analyzed the difference using a two-sample t-test. We used stratified simple linear regression to test the association of race with transgender and gender nonconforming identity and mental health distress. Participants were recruited from a network of community health centers in British Columbia, Canada. The quantitative sample (n = 727) had a mean age of 21 years (SD = 2), 48% were non-white, 51% were white, and 77% were from Metro Vancouver. Compared to cisgender young people, transgender and gender nonconforming young people reported significantly higher levels of mental health distress. Transgender and gender nonconforming youth were more distressed than cisgender youth across both race strata but non-white transgender and gender nonconforming young people were not more distressed than white transgender and gender nonconforming young people. The findings from this study emphasize the need for increased education and understanding of transgender and gender nonconforming concepts and health concerns as well as on promoting intersectoral collaboration of social services organizations beyond simply health care.
Parent-Infant Interactions Among Infants With High Risk of Cerebral Palsy: A Protocol for an Observational Study of Infant and Parental Factors for Dyadic Reciprocity
Authors: Røhder Katrine , Willerslev-Olsen Maria , Nielsen Jens Bo , Greisen Gorm , Harder Susanne
Published: September 2021

Background: An early diagnosis of chronic disability, such as risk of Cerebral Palsy (CP), is likely to affect the quality of parent-infant interactions by affecting both infant and parental factors. Due to adverse perinatal events, infants at high risk of CP may exhibit less engagement in interactions, while parents may experience increased mental health problems and disrupted parental representations that can have a negative effect on parental sensitivity. Recent clinical guidelines on early intervention among families with infants at risk of CP recommends supporting parental sensitivity and mutual enjoyable interactions more research is needed to inform such interventions. This includes understanding how infant and parental risk as well as resilience factors impact parent-infant interactions and how existing parenting programs developed among typical developing infants should be adapted to families with infants at risk of CP. In addition, as majority of research on infant neurohabilitation focus on improving motor and cognitive outcomes research on infant emotional development is needed. The study aim is to assess the quality of early parent-infant interactions in families with high-risk infants, compared to families with low-risk infants, and to explore how interaction quality is affected by infant and parental factors. Three potential mediating factors explaining the association between CP risk and less optimal parent-infant interactions will be explored: infant interactional capacities, parental mental health and well-being, and parents' representations of their child.

Methods: The prospective, longitudinal design will follow infants at high risk for CP and their parents and a control group at three time points from 15 weeks to 15 months corrected infant age (CA). Measures comprise infant developmental assessments, questionnaires and interviews with both parents, and global ratings of video-recorded parent-infant interactions.

Discussion: Study results will enhance our understanding of how parent-infant interactions may be affected by perinatal neurological risk and identify potential important mechanisms for observed associations. This knowledge could assist in planning future early screening and intervention programs and identifying families who should be offered targeted psychological interventions in addition to neurohabilitation programs.
Social Sciences in One Health: Insights From Multiple Worlds Perspectives on the Dam Rupture in Brumadinho-Brazil
Authors: Brandão Ana Pérola Drulla , Sussai Stefanie , Germine Jéssica Alves de Lima , Eltz Diego Duarte , Araújo Aline
Published: September 2021

Concepts that integrate human, animal, and ecosystem health - such as One Health (OH) - have been highlighted in recent years and mobilized in transdisciplinary approaches. However, there is a lack of input from the social sciences in OH discussions. This is a gap to overcome, including in Latin America. Therefore, this paper incorporates recent studies from economics and anthropology to the debate, contributing to the opening of transdisciplinary dialogues for the elaboration of OH theory and practice. As a starting point, we explore the recent case of a tailings dam breach, making considerations about how and why this event was experienced in different ways by the affected Indigenous and non-Indigenous worlds. From economics, we show how different theories perceive and impact these different worlds, presenting some existing alternatives to the hegemonic thinking of domination and exploitation. From anthropology, we present the perspectivism concept, deriving from the field of relational ontologies, suggesting there are significant and inevitable disagreements-equivocations-among different worlds. Thus, we discuss how the social sciences can help address challenging factors that need to be considered in health approaches that intend to deal with complex global problems. In conclusion, OH should incorporate social science discussions, considering relating practice to the multiple realities in which a particular problem or conflict is inserted. Overcoming the barriers that hinder transdisciplinary dialogue is fundamental and urgent for an effective approach to the multiple and distinct interconnections among humans, animals and environments.
An Introduction to Topological Data Analysis: Fundamental and Practical Aspects for Data Scientists
Authors: Chazal Frédéric , Michel Bertrand
Published: September 2021

With the recent explosion in the amount, the variety, and the dimensionality of available data, identifying, extracting, and exploiting their underlying structure has become a problem of fundamental importance for data analysis and statistical learning. Topological data analysis (tda) is a recent and fast-growing field providing a set of new topological and geometric tools to infer relevant features for possibly complex data. It proposes new well-founded mathematical theories and computational tools that can be used independently or in combination with other data analysis and statistical learning techniques. This article is a brief introduction, through a few selected topics, to basic fundamental and practical aspects of tda for nonexperts.
Turning Neural Prosthetics Into Viable Products
Authors: Loeb Gerald E. , Richmond Frances J.
Published: September 2021

Academic researchers concentrate on the scientific and technological feasibility of novel treatments. Investors and commercial partners, however, understand that success depends even more on strategies for regulatory approval, reimbursement, marketing, intellectual property protection and risk management. These considerations are critical for technologically complex and highly invasive treatments that entail substantial costs and risks in small and heterogeneous patient populations. Most implanted neural prosthetic devices for novel applications will be in FDA Device Class III, for which guidance documents have been issued recently. Less invasive devices may be eligible for the recently simplified “de novo” submission routes. We discuss typical timelines and strategies for integrating the regulatory path with approval for reimbursement, securing intellectual property and funding the enterprise, particularly as they might apply to implantable brain-computer interfaces for sensorimotor disabilities that do not yet have a track record of approved products.
Drivers for Intermittent Water Supply in India: Critical Review and Perspectives
Authors: Ghorpade Anujkumar , Sinha Abhishek Kumar , Kalbar Pradip P.
Published: September 2021

Intermittent Water Supply (IWS) is prevalent in most developing countries. Specifically, in India, IWS is existent throughout the country. Many studies focus on documenting the effects of IWS, and rarely the drivers of the IWS regime are studied. In this study, a systematic literature review was conducted on IWS studies around the globe. The various causes for IWS were documented. Then, by studying India's typical water supply system (WSS) configuration, the vicious cycle of IWS in India is discussed. Further, the drivers of IWS were identified and elaborated with the causing mechanisms. This knowledge will help devise strategies and solutions for improving the IWS in India and other developing countries with similar socio-economic conditions.
A Clinical Study Investigating Whether the Tongue-Out Position Improves the Quality of the Anatomical Appearance of the Pharynx on CT Imaging
Authors: Wu Jin-Long , Ge Rui-Gang , Tang Guang-Jian
Published: September 2021

Objective: To evaluate the effect of using the tongue-out position on the quality of the anatomical appearance of the pharynx on computed tomography (CT) images.

Methods: The data from enhanced CT thin-section images of the head and neck in 119 cases scanned were retrospectively analyzed. The cases were divided into two groups based on the position of the tip of the tongue on the images: the tongue-out group (63 cases) and non-tongue-out group (56 cases). Two observers separately evaluated the anatomy of the soft palate, uvula, palatine tonsils, epiglottis, epiglottic fossa, pyriform fossa, arytenoid folds, and tongue on all images. The Kappa test was applied to assess the consistency of scores between the two observers. In the case of data that satisfied the normal distribution, the significance of the difference in the average scores between the two groups was tested using an independent samples t-test with a value of p > 0.05. In the case of data that did not satisfy the normal distribution, the Mann–Whitney U test was adopted to test the significance of the difference in the average scores between the two groups using a value of p < 0.05. The number of cases with swallowing artifacts on the CT images in both groups was statistically analyzed and the chi-square test was used to determine whether the difference in the incidence of artifacts between the two groups was significant.

Results: The Kappa test showed good consistency between the two observers scoring of the soft palate, uvula, epiglottis, epiglottic fossa, pyriform fossa, aryepiglottic folds, and tongue. The image scores of the soft palate, uvula, epiglottis, epiglottic fossa, and tongue in the tongue-out group vs. the non-tongue-out group did not satisfy the normal distribution. The Mann–Whitney U test showed that the differences in the image scores between the two groups were statistically significant in all cases (p < 0.05). The incidence of swallowing artifacts in the tongue-out group and the non-tongue-out group was 15 and 32%, respectively. The result of the chi-square test showed that the difference in the incidence of swallowing artifacts between the two groups was statistically significant (p = 0.037).

Conclusion: The tongue-out position facilitated an improvement in the CT appearance of pharyngeal anatomy and was associated with a reduction in the incidence of swallowing artifacts.
In Silico Assessment of Safety and Efficacy of Screw Placement for Pediatric Image-Guided Otologic Surgery
Authors: Hermann Jan , Mueller Fabian , Weber Stefan , Caversaccio Marco , O'Toole Bom Braga Gabriela
Published: September 2021

Introduction: Current high-accuracy image-guided systems for otologic surgery use fiducial screws for patient-to-image registration. Thus far, these systems have only been used in adults, and the safety and efficacy of the fiducial screw placement has not yet been investigated in the pediatric population.

Materials and Methods: In a retrospective study, CT image data of the temporal region from 11 subjects meeting inclusion criteria (8–48 months at the time of surgery) were selected, resulting in n = 20 sides. These datasets were investigated with respect to screw stability efficacy in terms of the cortical layer thickness, and safety in terms of the distance of potential fiducial screws to the dura mater or venous sinuses. All of these results are presented as distributions, thickness color maps, and with descriptive statistics. Seven regions within the temporal bone were analyzed individually. In addition, four fiducial screws per case with 4 mm thread-length were placed in an additively manufactured model according to the guidelines for robotic cochlear implantation surgery. For all these screws, the minimal distance to the dura mater or venous sinuses was measured, or if applicable how much they penetrated these structures.

Results: The cortical layer has been found to be mostly between 0.7–3.3 mm thick (from the 5th to the 95th percentile), while even thinner areas exist. The distance from the surface of the temporal bone to the dura mater or the venous sinuses varied considerably between the subjects and ranged mostly from 1.1–9.3 mm (from the 5th to the 95th percentile). From all 80 placed fiducial screws of 4 mm thread length in the pediatric subject younger than two years old, 22 touched or penetrated either the dura or the sigmoid sinus. The best regions for fiducial placement would be the mastoid area and along the petrous pyramid in terms of safety. In terms of efficacy, the parietal followed by the petrous pyramid, and retrosigmoid regions are most suited.

Conclusion: The current fiducial screws and the screw placement guidelines for adults are insufficiently safe or effective for pediatric patients.
Investigating the Efficacy of a Canine Rabies Vaccine Following Storage Outside of the Cold-Chain in a Passive Cooling Device
Authors: Lugelo Ahmed , Hampson Katie , Czupryna Anna , Bigambo Machunde , McElhinney Lorraine M. , Marston Denise A. , Kazwala Rudovick , Lankester Felix
Published: September 2021

Background: Thermostable vaccines greatly improved the reach and impact of large-scale programmes to eliminate infectious diseases such as smallpox, polio, and rinderpest. A study from 2015 demonstrated that the potency of the Nobivac® Rabies vaccine was not impacted following experimental storage at 30°C for 3 months. Whether the vaccine would remain efficacious following storage under more natural, fluctuating temperature conditions remains unknown. We carried out a randomised controlled non-inferiority trial to compare serological responses in dogs following vaccination with doses stored under cold chain conditions with those stored within a locally made Passive Cooling Device (“Zeepot”) under fluctuating temperature conditions.

Materials and Methods: Nobivac® Rabies vaccine was stored under either cold-chain conditions or within the Zeepot for 2 months. Daily ambient temperatures and temperatures within the Zeepot were recorded every 3 h. Following storage, 412 domestic dogs were randomly assigned to receive either cold-chain or Zeepot stored Nobivac® Rabies vaccine. Baseline and day 28-post vaccination blood samples were collected. Serological analysis using the Fluorescent Antibody Virus Neutralisation assay was carried out with a threshold of 0.5 IU/ml to determine seroconversion. In addition, the impact of dog Body Condition Score, sex, and age on seroconversion was examined.

Results: The serological response of dogs vaccinated using Nobivac® Rabies vaccine stored within the Zeepot was not inferior to the response of dogs vaccinated using cold-chain stored vaccine (z = 1.1, df = 313, p-value = 0.25). Indeed, the 28-day post-vaccination group geometric mean titre was 1.8 and 2.0 IU/ml for cold-chain vs. non-cold-chain storage, respectively. Moreover, the percentage of dogs that seroconverted in each arm was almost identical (85%). There was a positive linear trend between Body Condition Score (O.R. 2.2, 95% CI: 1.1–5.1) and seroconversion, suggesting dogs of poor condition may not respond as expected to vaccination.

Conclusions: Our study demonstrated the potency of Nobivac® Rabies vaccine is not impacted following storage under elevated fluctuating temperatures within a Zeepot. These results have potentially exciting applications for scaling up mass dog vaccination programmes in low-and-middle income countries, particularly for hard-to-reach populations with limited access to power and cold-chain vaccine storage.
Clinical Evaluation of Corridor Disease in Bos indicus (Boran) Cattle Naturally Infected With Buffalo-Derived Theileria parva
Authors: Cook Elizabeth A. J. , Sitt Tatjana , Poole E. Jane , Ndambuki Gideon , Mwaura Stephen , Chepkwony Maurine C. , Latre de Late Perle , Miyunga Antoinette A. , van Aardt Richard , Prettejohn Giles , Wragg David , Prendergast James G. D. , Morrison W. Ivan , Toye Philip
Published: September 2021

Corridor disease (CD) is a fatal condition of cattle caused by buffalo-derived Theileria parva. Unlike the related condition, East Coast fever, which results from infection with cattle-derived T. parva, CD has not been extensively studied. We describe in detail the clinical and laboratory findings in cattle naturally infected with buffalo-derived T. parva. Forty-six cattle were exposed to buffalo-derived T. parva under field conditions at the Ol Pejeta Conservancy, Kenya, between 2013 and 2018. The first signs of disease observed in all animals were nasal discharge (mean day of onset was 9 days post-exposure), enlarged lymph nodes (10 days post-exposure), and pyrexia (13.7 days post-exposure). Coughing and labored breathing were observed in more than 50% of animals (14 days post-exposure). Less commonly observed signs, corneal edema (22%) and diarrhea (11%), were observed later in the disease progression (19 days post-exposure). All infections were considered clinically severe, and 42 animals succumbed to infection. The mean time to death across all studies was 18.4 days. The mean time from onset of clinical signs to death was 9 days and from pyrexia to death was 4.8 days, indicating a relatively short duration of clinical illness. There were significant relationships between days to death and the days to first temperature (chi2 = 4.00, p = 0.046), and days to peak temperature (chi2 = 25.81, p = 0.001), animals with earlier onset pyrexia died sooner. These clinical indicators may be useful for assessing the severity of disease in the future. All infections were confirmed by the presence of macroschizonts in lymph node biopsies (mean time to parasitosis was 11 days). Piroplasms were detected in the blood of two animals (4%) and 20 (43%) animals seroconverted. In this study, we demonstrate the successful approach to an experimental field study for CD in cattle. We also describe the clinical progression of CD in naturally infected cattle, including the onset and severity of clinical signs and pathology. Laboratory diagnoses based on examination of blood samples are unreliable, and alternatives may not be available to cattle keepers. The rapid development of CD requires recognition of the clinical signs, which may be useful for early diagnosis of the disease and effective intervention for affected animals.
Bi-Level Positive Airway Pressure for Non-invasive Respiratory Support of Foals
Authors: Raidal Sharanne L. , Catanchin Chee Sum Melanie , Burgmeestre Lexi , Quinn Chris T.
Published: September 2021

Respiratory insufficiency and pulmonary health are important considerations in equine neonatal care. As the majority of foals are bred for athletic pursuits, strategies for respiratory support of compromised foals are of particular importance. The administration of supplementary oxygen is readily implemented in equine practice settings, but does not address respiratory insufficiency due to inadequate ventilation and is no longer considered optimal care for hypoxia in critical care settings. Non-invasive ventilatory strategies including continuous or bi-level positive airway pressure are effective in human and veterinary studies, and may offer improved respiratory support in equine clinical practice. The current study was conducted to investigate the use of a commercial bi-level positive airway pressure (BiPAP) ventilator, designed for home care of people with obstructive respiratory conditions, for respiratory support of healthy foals with pharmacologically induced respiratory insufficiency. A two sequence (administration of supplementary oxygen with, or without, BiPAP), two phase, cross-over experimental design was used in a prospective study with six foals. Gas exchange and mechanics of breathing (increased tidal volume, decreased respiratory rate and increased peak inspiratory flow) were improved during BiPAP relative to administration of supplementary oxygen alone or prior studies using continuous positive airway pressure, but modest hypercapnia was observed. Clinical observations, pulse oximetry and monitoring of expired carbon dioxide was of limited benefit in identification of foals responding inappropriately to BiPAP, and improved methods to assess and monitor respiratory function are required in foals.
Case Report: Corpus Callosotomy in a Cat With Drug-Resistant Epilepsy of Unknown Cause
Authors: Hasegawa Daisuke , Asada Rikako , Mizuno Satoshi , Yu Yoshihiko , Hamamoto Yuji , Kanazono Shinichi
Published: September 2021

A 2-month-old, intact male domestic shorthair cat with dullness, bilateral central blindness, and recurrent epileptic seizures was presented to a local clinic. Seizures were the generalized myoclonic and tonic-clonic type. Phenobarbital was initiated and maintained; however, seizures were not controlled. Other anti-seizure drugs, including levetiracetam, zonisamide, and diazepam, also provided insufficient seizure control with seizures occurring hourly to daily. By 8 months of age, the cat displayed non-ambulatory tetraparesis and deep somnolence. Magnetic resonance imaging (MRI), cerebrospinal fluid analysis, and pre- and post-prandial total bile acid analyses were unremarkable. Scalp electroencephalography (EEG) revealed central dominant but generally synchronized spikes and multiple spikes. The cat was diagnosed with drug-resistant epilepsy of unknown cause and was included in a clinical trial of epilepsy surgery. Given the unremarkable MRI and bilateral synchronized EEG abnormalities, a corpus callosotomy was performed at 12 months of age, and partial desynchronization of spikes was confirmed on EEG. Incomplete transection was found in the genu of the corpus callosum on postoperative MRI. After surgery, the mental status and ambulation clearly improved, and seizure frequency and duration were remarkably reduced. Recheck with follow-up EEG and MRI were performed at 3, 6, and 12 months after surgery. Scores of activities of daily living and visual analog scales including cat's and owner's quality of life had also improved considerably. This case report is the first documentation of the one-year clinical outcome of corpus callosotomy in a clinical feline case with drug-resistant epilepsy.
XGB4mcPred: Identification of DNA N4-Methylcytosine Sites in Multiple Species Based on an eXtreme Gradient Boosting Algorithm and DNA Sequence Information
Authors: Wang Xiao , Lin Xi , Wang Rong , Fan Kai-Qi , Han Li-Jun , Ding Zhao-Yuan
Published: September 2021

DNA N4-methylcytosine(4mC) plays an important role in numerous biological functions and is a mechanism of particular epigenetic importance. Therefore, accurate identification of the 4mC sites in DNA sequences is necessary to understand the functional mechanism. Although some effective calculation tools have been proposed to identifying DNA 4mC sites, it is still challenging to improve identification accuracy and generalization ability. Therefore, there is a great need to build a computational tool to accurately identify the position of DNA 4mC sites. Hence, this study proposed a novel predictor XGB4mcPred, a predictor for the identification of 4mC sites trained using an extreme gradient boosting algorithm (XGBoost) and DNA sequence information. Firstly, we used the One-Hot encoding on adjacent and spaced nucleotides, dinucleotides, and trinucleotides of the original 4mC site sequences as feature vectors. Then, the importance values of the feature vectors pre-trained by the XGBoost algorithm were used as a threshold to filter redundant features, resulting in a significant improvement in the identification accuracy of the constructed XGB4mcPred predictor to identify 4mC sites. The analysis shows that there is a clear preference for nucleotide sequences between 4mC sites and non-4mC site sequences in six datasets from multiple species, and the optimized features can better distinguish 4mC sites from non-4mC sites. The experimental results of cross-validation and independent tests from six different species show that our proposed predictor XGB4mcPred significantly outperformed other state-of-the-art predictors and was improved to varying degrees compared with other state-of-the-art predictors. Additionally, the user-friendly webserver we used to developed the XGB4mcPred predictor was made freely accessible.
Adoption and Diffusion of Agroecological Practices in the Horticulture of Catalonia
Authors: Polonio Punzano Adrián , Rahmani Djamel , Cabello Delgado Maria del Mar
Published: September 2021

The environmental impact of conventional food production systems imposes a rapid transition towards sustainable production systems through the adoption of agroecological practices. The barriers and accelerators of the adoption of agroecological practices were identified for horticultural crops in Catalonia. Eight interviews and thirty surveys were conducted with local producers. Results show that the loss of producer income and the lack of social awareness regarding organic products are among the important barriers to the adoption of agroecological practices, while information about the experience of other farmers is considered a motivational factor. Finally, the study concludes that the adoption of agroecological practices has economic, political, social, academic and agronomic components.
Prevalence of Varied Coat Coloration in a Yellow-Throated Marten (Martes flavigula) Population
Authors: Gong Yinan , Zhao Guojing , Yang Huixin , Li Yan , Tan Mengyu , Wang Ning , Ge Jianping , Yang Haitao , Feng Limin
Published: September 2021

Mammalian coat color is determined by heritable variations such as disease, nutrition, and hormone levels. Variation in animal coat color is also considered an environmental indicator and provides clues for the study of population genetics and biogeography. Records of abnormal coloration in the wild are rare, not only because it is often selected against, but also because of the difficulties in detection of the phenomenon. We used long-term camera-trapping data to first report abnormal coat coloration in yellow-throated marten (Martes flavigula) in China. Six types of abnormal coloration were found only in the Northeast Tiger and Leopard National Park, Northeast China, which were not reported in other regions in China. A total of 268 videos of Martes flavigula contained normal coloration, 455 videos of individuals of the species contained abnormal coloration, 437 contained the ‘gloving’ type (martens with de-pigmented front toes, paws or lower forelimbs), while the remaining other 18 videos contained five types (different degrees of white-spotting and dilution). The higher relative abundance index (0.428, ‘gloving’ to 0.329, normal) and wide distribution area of the ‘gloving’ type indicated that this abnormal coat coloration type is usual in Northeast China, which may reflect genetic variability in the local population. These records will contribute to further research on animal coat color and its corresponding adaptive strategy.
The Impact of Genetics on Gut Microbiota of Growing and Fattening Pigs under Moderate N Restriction
Authors: Sarri Laura , Costa-Roura Sandra , Balcells Joaquim , Seradj Ahmad Reza , de la Fuente Gabriel
Published: September 2021

Characterization of intestinal microbiota is of great interest due to its relevant impact on growth, feed efficiency and pig carcass quality. Microbial composition shifts along the gut, but it also depends on the host (i.e., age, genetic background), diet composition and environmental conditions. To simultaneously study the effects of producing type (PT), production phase (PP) and dietary crude protein (CP) content on microbial populations, 20 Duroc pigs and 16 crossbred pigs (F2), belonging to growing and fattening phases, were used. Half of the pigs of each PT were fed a moderate CP restriction (2%). After sacrifice, contents of ileum, cecum and distal colon were collected for sequencing procedure. Fattening pigs presented higher microbial richness than growing pigs because of higher maturity and stability of the community. The F2 pigs showed higher bacterial alpha diversity and microbial network complexity (cecum and colon), especially in the fattening phase, while Duroc pigs tended to have higher Firmicutes/Bacteroidetes ratio in cecum segment. Lactobacillus was the predominant genus, and along with Streptococcus and Clostridium, their relative abundance decreased throughout the intestine. Although low CP diet did not alter the microbial diversity, it increased interaction network complexity. These results have revealed that the moderate CP restriction had lower impact on intestinal microbiota than PP and PT of pigs.
An Experimental Model for Iron Deficiency Anemia in Sows and Offspring Induced by Blood Removal during Gestation
Authors: Rydal Martin Peter , Bhattarai Sheeva , Nielsen Jens Peter
Published: September 2021

Anemia is a common condition in sow herds. We aimed to study the effects of severe iron deficiency during gestation on sow and piglet health outcomes with an experimental model for blood-removal-induced iron deficiency anemia. In total, 18 multiparous sows (8 in trial I and 10 in trial II) were allocated to either a blood removal group or a control group. Hematologic parameters were monitored at regular intervals and the tissue iron concentrations were measured for the sows and newborn piglets after farrowing. In trial I, the mean liver iron content was reduced to 46.7 µg/g in the blood removal sows compared to 252.6 µg/g in the controls (p < 0.001). In trial II, sows in the blood removal group had lower iron content in the liver (147.8 µg/g), kidney (46.3 µg/g) and spleen (326.5 µg/g) compared to the control sows (323.2 µg/g, 81.3 µg/g and 728.9 µg/g, respectively; p = 0.009, 0.016, 0.01, respectively). In trial I, piglets from sows in the blood removal group had significantly decreased hematocrit (Hct), red blood cells (RBC) and a tendency for reduced hemoglobin (Hb) compared to the control piglets. We established a blood removal model that resulted in mild- to severe degrees of sow anemia and reduced tissue iron stores at farrowing.
Pterostilbene Attenuates Particulate Matter-Induced Oxidative Stress, Inflammation and Aging in Keratinocytes
Authors: Teng Wei-Lin , Huang Pao-Hsien , Wang Hui-Chun , Tseng Chih-Hua , Yen Feng-Lin
Published: September 2021

Particulate matter (PM) is the main indicator of air pollutants, and it may increase the level of reactive oxygen species (ROS) in keratinocytes, leading to skin inflammation, aging, and decreased moisturizing ability. Pterostilbene (PTS) is a dimethylated analog of resveratrol that has antioxidant effects. However, the molecular mechanisms of PTS in preventing PM-induced keratinocyte inflammation and aging have not been investigated yet. Therefore, we used PM-induced human keratinocytes to investigate the protective mechanisms of PTS. The results showed that 20 μM PTS had no toxicity to HaCaT keratinocytes and significantly reduced PM-induced intracellular ROS production. In addition, nuclear translocation of the aryl hydrocarbon receptor (AHR) was inhibited by PTS, leading to reduced expression of its downstream CYP1A1. PTS further inhibited PM-induced MAPKs, inflammation (COX-2), and aging (MMP-9) protein cascades, and rescued moisturizing (AQP-3) protein expression. We analyzed the PTS content in cells at different time points and compared the concentration required for PTS to inhibit the target proteins. Finally, we used the skin penetration assay to show that the PTS essence mainly exists in the epidermal layer and did not enter the system circulation. In conclusion, PTS could protect HaCaT keratinocytes from PM-induced damage and has the potential to become a cosmetic ingredient.
Optimization of Pulsed Electric Field as Standalone “Green” Extraction Procedure for the Recovery of High Value-Added Compounds from Fresh Olive Leaves
Authors: Pappas Vasileios M. , Lakka Achillia , Palaiogiannis Dimitrios , Athanasiadis Vassilis , Bozinou Eleni , Ntourtoglou George , Makris Dimitris P. , Dourtoglou Vassilis G. , Lalas Stavros I.
Published: September 2021

Olive leaves (OLL) are reported as a source of valuable antioxidants and as an agricultural by-product/waste. Thus, a twofold objective with multi-level cost and environmental benefits arises for a “green” standalone extraction technology. This study evaluates the OLL waste valorization through maximizing OLL extracts polyphenol concentration utilizing an emerging “green” non-thermal technology, Pulsed Electric Field (PEF). It also provides further insight into the PEF assistance span for static solid-liquid extraction of OLL by choosing and fine-tuning important PEF parameters such as the extraction chamber geometry, electric field strength, pulse duration, pulse period (and frequency), and extraction duration. The produced extracts were evaluated via comparison amongst them and against extracts obtained without the application of PEF. The Folin-Ciocalteu method, high-performance liquid chromatography, and differential scanning calorimetry were used to determine the extraction efficiency. The optimal PEF contribution on the total polyphenols extractability (38% increase with a 117% increase for specific metabolites) was presented for rectangular extraction chamber, 25% v/v ethanol:water solvent, pulse duration (tpulse) 2 μs, electric field strength (E) 0.85 kV cm−1, 100 μs period (Τ), and 15 min extraction duration (textraction), ascertaining a significant dependence of PEF assisting extraction performance to the parameters chosen.
Weighted Centrality and Retail Store Locations in Beijing, China: A Temporal Perspective from Dynamic Public Transport Flow Networks
Authors: Liao Cong , Dai Teqi , Zhao Pengfei , Ding Tiantian
Published: September 2021

The spatial relationship between transport networks and retail store locations is an important topic in studies related to commercial activities. Much effort has been made to study physical street networks, but they are seldom empirically discussed with considerations of transport flow networks from a temporal perspective. By using Beijing’s bus and subway smart card data (SCD) and point of interest (POI) data, this study examined the location patterns of various retail stores and their daily dynamic relationships with three weighted centrality indices in the networks of public transport flows: degree, betweenness, and closeness. The results indicate that most types of retail stores are highly correlated with weighted centrality indices. For the network constructed by total public transport flows in the week, supermarkets, convenience stores, electronics stores, and specialty stores had the highest weighted degree value. By contrast, building material stores and shopping malls had the weighted closeness and weighted betweenness values, respectively. From a temporal perspective, most retail types’ largest correlations on weekdays occurred during the after-work period of 19:00 to 21:00. On weekends, shopping malls and electronics stores changed their favorite periods to the daytime, while specialty stores favored the daytime on both weekdays and weekends. In general, the higher store type level of the shopping malls correlates more to weighted closeness or betweenness, and the lower-level store type of convenience stores correlates more to weighted degree. This study provides a temporal analysis that surpasses previous studies on street centrality and can help with urban commercial planning.
Simulation and Experimental Research on the Disturbance Behavior of a Sun-Tracking Solar Array Driven by a Stepping Motor
Authors: Yu Jisong , Guan Yongliang , Zhang Daowei , Chen Shanbo , Zhao Chunjuan , Zhang Lei
Published: September 2021

A sun-tracking solar array is an effective solution to the increasing energy demand of spacecrafts. However, the driving torque fluctuation of the solar array driving assembly (SADA) and its oscillatory motion lead to the micro-vibration problem of the spacecraft. In this article, a disturbance torque model of the sun-tracking solar array, which takes its friction and flexibility into consideration, is established. Furthermore, a test platform was built to measure the disturbance force/torque, and a solar array simulator (SAS) was designed to replace the solar array in the ground experiments. Finally, the disturbance torque of the SADA-driven SAS is simulated, and the model is validated by comparing the simulation results with the experimental results. These results show that the frequency error of the model is less than 0.648%, and the amplitude error of the corresponding frequency is less than 22.33%, which indicates that the proposed model can effectively predict the disturbance torque generated by the sun-tracking solar array in orbit. The research provides theoretical guidance for the system optimization design and micro-vibration suppression of spacecrafts.
Customized Approach to Greenhouse Gas Emissions Calculations in Railway Freight Transport
Authors: Chocholac Jan , Hruska Roman , Machalik Stanislav , Sommerauerova Dana , Krupka Jiri
Published: September 2021

The topic of global warming is and will continue to be a crucial topic of this millennium. Freight transport, as a producer of greenhouse gas (hereinafter GHG) emissions, makes a significant contribution to the greenhouse effect. Large supply chains and large volumes of freight transport, which imply the production of significant volumes of GHG emissions, characterize the automotive industry (hereinafter AI). Thanks to these premises, it is necessary to seek and develop tools for reducing the volume of GHG emissions produced from the logistic activities of the AI, while maintaining the required level of logistic services. The assumptions for the calculation of GHG emissions from railway freight transport (hereinafter RFT) in the AI were identified through the use of semi-structured interviewing. Available railway freight GHG emission calculators were identified and analyzed from the perspective of suitability for the AI using a comparative content analysis. The main result of this manuscript is the proposal of a fully customized approach to GHG emission calculations in RFT for the AI. This approach was proposed, applied, and verified in the form of an interpretative case study. The use of this approach can be expected in support of logistic planning and decision making.
An Evolutionary-Based Sentiment Analysis Approach for Enhancing Government Decisions during COVID-19 Pandemic: The Case of Jordan
Authors: Obiedat Ruba , Harfoushi Osama , Qaddoura Raneem , Al-Qaisi Laila , Al-Zoubi Ala’ M.
Published: September 2021

The world has witnessed recently a global outbreak of coronavirus disease (COVID-19). This pandemic has affected many countries and has resulted in worldwide health concerns, thus governments are attempting to reduce its spread and impact on different aspects of life such as health, economics, education, and politics by making emergent decisions and policies (e.g., lockdown and social distancing). These new regulations influenced people’s daily life and cast significant burdens, concerns, and disparities on various population groups. Taking the wrong actions and enforcing bad decisions by some countries result in increasing the contagion rate and more catastrophic results. People start to post their opinions and feelings about their government’s decisions on different social media networks, and the data received through these platforms present a very useful source of information that affects how governments perceive and cope with the current the pandemic. Jordan was one of the top affected countries. In this paper, we proposed a decision support system based on the sentiment analysis mechanism by combining support vector machines with a whale optimization algorithm for automatically tuning the hyperparameters and performing feature weighting. The work is based on a hybrid evolutionary approach that aims to perform sentiment analysis combined with a decision support system to study people’s posts on Facebook to investigate their attitudes and feelings toward the government’s decisions during the pandemic. The government regulations were divided into two periods: the first and latter regulations. Studying public sentiments during these periods allows decision-makers in the government to sense people’s feelings, alert them in case of possible threats, and help in making proactive actions if needed to better handle the current pandemic situation. Five different versions were generated for each of the two collected datasets. The results demonstrate the superiority of the proposed Whale Optimization Algorithm & Support Vector Machines (WOA-SVM) against other metaheuristic algorithms and standard classification models as WOA-SVM has achieved 78.78% in terms of accuracy and 84.64% in term of f-measure, while other standard classification models such as NB, k-NN, J84, and SVM achieved an accuracy of 69.25%, 69.78%, 70.17%, and 69.29%, respectively, with 64.15%, 62.90%, 60.51%, and 59.09% F-measure. Moreover, when comparing our proposed WOA-SVM approach with other metaheuristic algorithms, which are GA-SVM, PSO-SVM, and MVO-SVM, WOA-SVM proved to outperform the other approaches with results of 78.78% in terms of accuracy and 84.64% in terms of F-measure. Further, we investigate and analyze the most relevant features and their effect to improve the decision support system of government decisions.
Elastic Properties Estimation of Masonry Walls through the Propagation of Elastic Waves: An Experimental Investigation
Authors: Marazzani Jacopo , Cavalagli Nicola , Gusella Vittorio
Published: September 2021

Structural identification is one of the most important steps when dealing with historic buildings. Knowledge of the parameters, which define the mechanical properties of these kinds of structures, is fundamental in preparing interventions aimed at their restoration and strengthening, especially if they have suffered damage due to strong events. In particular, by using non-destructive techniques it is possible to estimate the mechanical characteristics of load-bearing structures without compromising the artistic value of the monumental buildings. In this paper, after recalling the main theoretical aspects, the use of elastic waves propagation through impact tests for the characterization of the masonry walls of a monumental building is described. The impact test allowed us to estimate the elastic characteristics of the homogeneous solid equivalent to masonry material. This confirms the great potential of the non-destructive diagnostics suitable for analyzing important structural parameters without affecting the preservation of historical masonry structures.
Fabrication and Characterization of Bio-Epoxy Eggshell Composites
Authors: Owuamanam Stephen , Soleimani Majid , Cree Duncan E.
Published: September 2021

In this study, an innovative composite was fabricated in which the matrix is partially derived from natural sources and the filler from undervalued eggshell waste material. The effect of coating eggshells and mineral limestone with 2 wt.% stearic acid on the mechanical properties of a bio-epoxy matrix was investigated. Eggshells and limestone (untreated and stearic acid-treated) fillers were added to the bio-epoxy matrix in quantities of 5, 10, and 20 wt.% loadings using a solution mixing technique. The CaCO3 content in eggshells was confirmed to be 88 wt.%, and the crystalline phase was found to be calcite. The stearic acid coating did not show any decrease in crystallinity of the fillers. Scanning electron microscopy (SEM) displayed changes in the fractured surfaces, which infers the fillers altered the bio-epoxy polymer. The mechanical property results showed enhancements in the composite tensile modulus and flexural modulus compared to the pure bio-epoxy, as expected. In contrast, despite the improvement in the tensile and flexural strengths of the stearic acid-treated fillers, the composite strength values were not higher than those of the unfilled bio-epoxy matrix. The energy absorbed by all composites in Charpy impact tests fell below that of the pure bio-epoxy and decreased with an increase in filler content for both untreated and stearic acid-treated fillers tested at 23 and −40 °C. Statistical analysis of the results was conducted using Statistical Analysis Software (SAS) with ranking based on Tukey’s method. The study identified that the addition of 5, 10, and 20 wt.% in a bio-epoxy matrix may be acceptable provided the end product requires lower tensile and flexural load requirements than those of the pure bio-epoxy. However, filler loadings below 5 wt.% would be a better choice.