bluuebunny/crossref_metadata_2025 huggingface dataset:
Nepenthes Ethyl Acetate Extract Provides Oxidative Stress-Dependent Anti-Leukemia Effects
Authors: Liu Wangta , Lin Li-Ching , Wang Pei-Ju , Chen Yan-Ning , Wang Sheng-Chieh , Chuang Ya-Ting , Tsai I-Hsuan , Yu Szu-Yin , Chang Fang-Rong , Cheng Yuan-Bin , Huang Li-Chen , Huang Ming-Yii , Chang Hsueh-Wei
Published: September 2021

Several kinds of solvents have been applied to Nepenthes extractions exhibiting antioxidant and anticancer effects. However, they were rarely investigated for Nepenthes ethyl acetate extract (EANT), especially leukemia cells. The purpose of the present study was to evaluate the antioxidant properties and explore the antiproliferation impact and mechanism of EANT in leukemia cells. Five standard assays demonstrated that EANT exhibits antioxidant capability. In the cell line model, EANT dose-responsively inhibited cell viabilities of three leukemia cell lines (HL-60, K-562, and MOLT-4) based on 24 h MTS assays, which were reverted by pretreating oxidative stress and apoptosis inhibitors (N-acetylcysteine and Z-VAD-FMK). Due to similar sensitivities among the three cell lines, leukemia HL-60 cells were chosen for exploring antiproliferation mechanisms. EANT caused subG1 and G1 cumulations, triggered annexin V-detected apoptosis, activated apoptotic caspase 3/7 activity, and induced poly ADP-ribose polymerase expression. Moreover, reactive oxygen species, mitochondrial superoxide, and mitochondrial membrane depolarization were generated by EANT, which was reverted by N-acetylcysteine. The antioxidant response to oxidative stress showed that EANT upregulated mRNA expressions for nuclear factor erythroid 2-like 2 (NFE2L2), catalase (CAT), thioredoxin (TXN), heme oxygenase 1 (HMOX1), and NAD(P)H quinone dehydrogenase 1 (NQO1) genes. Moreover, these oxidative stresses led to DNA damage (γH2AX and 8-hydroxy-2-deoxyguanosine) and were alleviated by N-acetylcysteine. Taken together, EANT demonstrated oxidative stress-dependent anti-leukemia ability to HL-60 cells associated with apoptosis and DNA damage.
Inhibition of Breast Tumour Growth with Intravenously Administered PRKCA siRNA- and PTEN Tumour Suppressor Gene-Loaded Carbonate Apatite Nanoparticles
Authors: Ibnat Nabilah , Islam Rowshan Ara , Chowdhury Ezharul Hoque
Published: September 2021

Gene therapy aims to silence an oncogene through RNA interference, or replace an abnormal tumour suppressor via gene augmentation. In this study, we intended RNA interference for PRKCA oncogene and gene augmentation for PTEN tumour suppressor with a view to reduce tumour growth in a mouse model of breast cancer. Inorganic carbonate apatite nanoparticles (CA NPs) were utilized to deliver the synthetic siRNA and the purified gene-carrying plasmid DNA both in vitro and in vivo. Effects of PRKCA siRNA- and PTEN plasmid-loaded NPs on viability of MCF-7, MDA-MB-231 and 4T1 breast cancer cells were assessed by MTT assay. The cell viability data in MCF-7 cell line demonstrated that combined delivery of PRKCA specific siRNA and PTEN plasmid with CA NPs had an additive effect to significantly decrease cellular growth compared to individual treatments. In addition, we observed a similar pattern of cumulative influence for combined treatment in triple negative MDA-MB-231 breast cancer cell line. Upon treatment with PRKCA siRNA+PTEN plasmid-loaded NPs, a remarkable decrease in the phosphorylated form of AKT protein of PI3K/AKT pathway was observed in Western blot, indicative of diminished proliferative signal. Moreover, in vivo study in MCF-7 xenograft breast cancer mouse model demonstrated that the rate of growth and final tumour volume were reduced significantly in the mouse group that received intravenous treatment of PRKCA siRNA+NPs, and PTEN plasmid+NPs. Our findings demonstrated that PRKCA siRNA and PTEN plasmid loaded into CA NPs attenuated breast tumour growth, suggesting their therapeutic potential in the treatment of breast cancer.
Numerical Analyses on the Behavior of Geosynthetic-Reinforced Soil: Integral Bridge and Integrated Bridge System
Authors: Won Myoung-Soo , Langcuyan Christine Patinga
Published: September 2021

Geosynthetic-reinforced soil (GRS) technology has been used worldwide since the 1970s. An extension to its development is the application as a bridge abutment, which was initially developed by the Federal Highway Administration (FHWA) in the United States, called the GRS—integrated bridge system (GRS-IBS). Now, there are several variations of this technology, which includes the GRS Integral Bridge (GRS-IB) developed in Japan in the 2000s. In this study, the GRS-IB and GRS-IBS are examined. The former uses a GRS bridge abutment with a staged-construction full height rigid (FHR) facing integrated to a continuous girder on top of the FHR facings. The latter uses a block-faced GRS bridge abutment that supports the girders without bearings. In addition, a conventional integral bridge (IB) is considered for comparison. The numerical analyses of the three bridges using Plaxis 2D under static and dynamic loadings are presented. The results showed that the GRS-IB exhibited the least lateral displacement (almost zero) at wall facing and vertical displacements increments at the top of the abutment compared to those of the GRS-IBS and IB. The presence of the reinforcements (GRS-IB) reduced the vertical displacement increments by 4.7 and 1.3 times (max) compared to IB after the applied general traffic and railway loads, respectively. In addition, the numerical results revealed that the GRS-IB showed the least displacement curves in response to the dynamic load. Generally, the results revealed that the GRS-IB performed ahead of both the GRS-IBS and IB considering the internal and external behavior under static and dynamic loading.
Logistic Wavelets and Their Application to Model the Spread of COVID-19 Pandemic
Authors: Rza̧dkowski Grzegorz , Figlia Giuseppe
Published: September 2021

In the present paper, we model the cumulative number of persons, reported to be infected with COVID-19 virus, by a sum of several logistic functions (the so-called multilogistic function). We introduce logistic wavelets and describe their properties in terms of Eulerian numbers. Moreover, we implement the logistic wavelets into Matlab’s Wavelet Toolbox and then we use the continuous wavelet transform (CWT) to estimate the parameters of the approximating multilogistic function. Using the examples of several countries, we show that this method is effective as a method of fitting a curve to existing data. However, it also has a predictive value, and, in particular, allows for an early assessment of the size of the emerging new wave of the epidemic, thus it can be used as an early warning method.
Open Localization in 3D Package with TSV Daisy Chain Using Magnetic Field Imaging and High-Resolution Three-Dimensional X-ray Microscopy
Authors: Chen Yuan , Lai Ping , Huang Hong-Zhong , Zhang Peng , Lin Xiaoling
Published: September 2021

With the development of 3D integrated packaging technology, failure analysis is facing more and more challenges. Defect localization in a 3D package is a key step of failure analysis. The complex structure and materials of 3D package devices demand non-destructive defect localization technology for full packages. Magnetic field imaging and three-dimensional X-ray technology are not affected by package material or form. They are effective methods to realize defect localization on 3D packages. In this paper, magnetic field imaging and high-resolution three-dimensional X-ray microscopy were used to localize the open defect in a 3D package with a TSV daisy chain. A two-probe RF method in magnetic field imaging was performed to resolve isolation of the defect difficulties resulting from many different branches of TSV daisy chains. Additionally, a linear decay method was used to target sub-micron resolution at a long working distance. Multiple partition scans from a high-resolution 3D X-ray microscopy with a two-stage magnification structure were used to achieve sub-micron resolution. The open location identified by magnetic field imaging was consistent with that identified by a three-dimensional X-ray microscope. The opening was located on the top metal in the proximity of the fifth via. Physical failure analysis revealed the presence of a crack in the top metal at the opening location.
A Novel Principal Component Analysis Integrating Long Short-Term Memory Network and Its Application in Productivity Prediction of Cutter Suction Dredgers
Authors: Yang Ke , Yuan Jun-Lang , Xiong Ting , Wang Bin , Fan Shi-Dong
Published: September 2021

Dredging is a basic construction for waterway improvement, harbor basin maintenance, land reclamation, environmental protection dredging, and deep-sea mining. The dredging process of cutter suction dredgers is so complex that the operational data show strong characteristics of dynamic, nonlinearity, and time delay, which make it difficult to predict the productivity accurately via basic principles models. In this paper, we propose a novel integrating PCA-LSTM model to improve the productivity prediction of cutter suction dredger. Firstly, multiple variables are reduced in dimension and selected by PCA method based on the working mechanism of cutter suction dredger. Then the productivity is predicted via mud concentration in long short-term memory network with relevant operational time-series data. Finally, the proposed method is successfully applied to an actual case study in China. Also, it performs well in the cross-validation and comparative study for several important characteristics: (i) it involves the operational parameters based on the mechanism analysis; and (ii) it is a deep-learning-based approach that can deal with operation series data with a special memory mechanism. This study provides a heuristic idea for integrating the data-driven method and supervision of human knowledge for application in practical engineering.
Hemodynamic Analysis on the Anastomosis Angle in Arteriovenous Graft Using Multiphase Blood Model
Authors: Kim Ji Tae , Kim Hyangkyoung , Ryou Hong Sun
Published: September 2021

Numerical analysis was performed for the effect of the venous anastomosis angle in a forearm arteriovenous graft for hemodialysis using a multiphase blood model. The geometry of the blood vessel was generated based on the patient-computed tomography data. The anastomosis angles were set at 15°, 30°, and 45°. The hematocrit was set at 34%, 45%, and 58%. The larger anastomosis angle, high wall shear stress area >11 Pa, increases to the side of the vein wall away from the anastomosis site. Further, the relatively low wall shear stress area, <3 Pa, occurs near the anastomosis site in larger anastomosis angles. Therefore, the effect of high wall shear stress has advantages in the vicinity of the anastomosis, as the anastomosis angle is larger, but disadvantages as the distance from the anastomosis increases. Moreover, patients with low hematocrit are advantageous for WSS area.
Valuation of Local Demand for Improved Air Quality: The Case of the Mae Moh Coal Mine Site in Thailand
Authors: Srisawasdi Worawat , Tsusaka Takuji W. , Winijkul Ekbordin , Sasaki Nophea
Published: September 2021

While the district of Mae Moh, Thailand has been well known for its atmospheric pollution associated with coal power production, economic assessment of demand for improved air quality has not been conducted to date. This study estimated local residents’ individual and aggregate willingness to pay (WTP) for mitigation of atmospheric pollution in Mae Moh using the contingent valuation method (CVM), and analyzed the factors associated with the individual WTP using the bivariate tobit and double-hurdle regression techniques. Primary data were collected through face-to-face interviews with a stratified sample of 200 residents. The hypothetical scenarios used in the CVM module were 50% and 80% mitigation of atmospheric concentrations of major pollutants. The weighted average WTP was found to be THB 251.3 and 307.9 per annum (USD 8.4 and 10.3) for the 50% and 80% reduction scenarios, respectively. The aggregate WTP for the entire population of Mae Moh was THB 10,008,733 and 12,264,761 per annum (USD 336,294 and 412,096), respectively. Education, occupation type, income, expenses, satisfaction with ambient quality, and perceived sources of pollution had significant associations with the individual WTP. The paper concludes by discussing policy implications for atmospheric pollution management and avenues for future research.
Repurposing Small Molecules to Target PPAR-γ as New Therapies for Peripheral Nerve Injuries
Authors: Rayner Melissa L. D. , Healy Jess , Phillips James B.
Published: September 2021

The slow rate of neuronal regeneration that follows peripheral nerve repair results in poor recovery, particularly where reinnervation of muscles is delayed, leading to atrophy and permanent loss of function. There is a clear clinical need to develop drug treatments that can accelerate nerve regeneration safely, restoring connections before the target tissues deteriorate irreversibly. The identification that the Rho/Rho-associated kinase (ROCK) pathway acts to limit neuronal growth rate is a promising advancement towards the development of drugs. Targeting Rho or ROCK directly can act to suppress the activity of this pathway; however, the pathway can also be modulated through the activation of upstream receptors; one of particular interest being peroxisome proliferator-activated receptor gamma (PPAR-γ). The connection between the PPAR-γ receptor and the Rho/ROCK pathway is the suppression of the conversion of inactive guanosine diphosphate (GDP)-Rho to active guanosine triphosphate GTP-Rho, resulting in the suppression of Rho/ROCK activity. PPAR-γ is known for its role in cellular metabolism that leads to cell growth and differentiation. However, more recently there has been a growing interest in targeting PPAR-γ in peripheral nerve injury (PNI). The localisation and expression of PPAR-γ in neural cells following a PNI has been reported and further in vitro and in vivo studies have shown that delivering PPAR-γ agonists following injury promotes nerve regeneration, leading to improvements in functional recovery. This review explores the potential of repurposing PPAR-γ agonists to treat PNI and their prospective translation to the clinic.
Revealing the Mutation Patterns of Drug-Resistant Reverse Transcriptase Variants of Human Immunodeficiency Virus through Proteochemometric Modeling
Authors: Qiu Jingxuan , Tian Xinxin , Liu Jiangru , Qin Yulong , Zhu Junjie , Xu Dongpo , Qiu Tianyi
Published: September 2021

Drug-resistant cases of human immunodeficiency virus (HIV) nucleoside reverse transcriptase inhibitors (NRTI) are constantly accumulating due to the frequent mutations of the reverse transcriptase (RT). Predicting the potential drug resistance of HIV-1 NRTIs could provide instructions for the proper clinical use of available drugs. In this study, a novel proteochemometric (PCM) model was constructed to predict the drug resistance between six NRTIs against different variants of RT. Forty-seven dominant mutation sites were screened using the whole protein of HIV-1 RT. Thereafter, the physicochemical properties of the dominant mutation sites can be derived to generate the protein descriptors of RT. Furthermore, by combining the molecular descriptors of NRTIs, PCM modeling can be constructed to predict the inhibition ability between RT variants and NRTIs. The results indicated that our PCM model could achieve a mean AUC value of 0.946 and a mean accuracy of 0.873 on the external validation set. Finally, based on PCM modeling, the importance of features was calculated to reveal the dominant amino acid distribution and mutation patterns on RT, to reflect the characteristics of drug-resistant sequences.
Small Extracellular Vesicle Enrichment of a Retrotransposon-Derived Double-Stranded RNA: A Means to Avoid Autoinflammation?
Authors: Barrios Marilou H. , Garnham Alexandra L. , Foers Andrew D. , Cheng-Sim Lesley , Masters Seth L. , Pang Ken C.
Published: September 2021

Small extracellular vesicles (SEVs) such as exosomes are released by multiple cell types. Originally believed to be a mechanism for selectively removing unwanted cellular components, SEVs have received increased attention in recent years for their ability to mediate intercellular communication. Apart from proteins and lipids, SEVs contain RNAs, but how RNAs are selectively loaded into SEVs remains poorly understood. To address this question, we profiled SEV RNAs from mouse dendritic cells using RNA-Seq and identified a long noncoding RNA of retroviral origin, VL30, which is highly enriched (>200-fold) in SEVs compared to parental cells. Bioinformatic analysis revealed that exosome-enriched isoforms of VL30 RNA contain a repetitive 26-nucleotide motif. This repeated motif is itself efficiently incorporated into SEVs, suggesting the likelihood that it directly promotes SEV loading. RNA folding analyses indicate that the motif is likely to form a long double-stranded RNA hairpin and, consistent with this, its overexpression was associated with induction of a potent type I interferon response. Taken together, we propose that preferential loading into SEVs of the VL30 RNA containing this immunostimulatory motif enables cells to remove a potentially toxic RNA and avoid autoinflammation. In this way, the original notion of SEVs as a cellular garbage bin should not be entirely discounted.
Rutaecarpine Increases Anticancer Drug Sensitivity in Drug-Resistant Cells through MARCH8-Dependent ABCB1 Degradation
Authors: Zou Tingting , Zeng Cheng , Qu Junyan , Yan Xiaohua , Lin Zhenghong
Published: September 2021

The overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) subfamily B member 1 (ABCB1; P-glycoprotein; MDR1) in some types of cancer cells is one of the mechanisms responsible for the development of multidrug resistance (MDR), which leads to the failure of chemotherapy. Therefore, it is important to inhibit the activity or reduce the expression level of ABCB1 to maintain an effective intracellular level of chemotherapeutic drugs. In this study, we found that rutaecarpine, a bioactive alkaloid isolated from Evodia Rutaecarpa, has the capacity to reverse ABCB1-mediated MDR. Our data indicated that the reversal effect of rutaecarpine was related to the attenuation of the protein level of ABCB1. Mechanistically, we demonstrated that ABCB1 is a newly discovered substrate of E3 ubiquitin ligase membrane-associated RING-CH 8 (MARCH8). MARCH8 can interact with ABCB1 and promote its ubiquitination and degradation. In short, rutaecarpine increased the degradation of ABCB1 protein by upregulating the protein level of MARCH8, thereby antagonizing ABCB1-mediated MDR. Notably, the treatment of rutaecarpine combined with other anticancer drugs exhibits a therapeutic effect on transplanted tumors. Therefore, our study provides a potential chemotherapeutic strategy of co-administrating rutaecarpine with other conventional chemotherapeutic agents to overcome MDR and improve therapeutic effect.
MRI-Determined Psoas Muscle Fat Infiltration Correlates with Severity of Weight Loss during Cancer Cachexia
Authors: Patzelt Lisa , Junker Daniela , Syväri Jan , Burian Egon , Wu Mingming , Prokopchuk Olga , Nitsche Ulrich , Makowski Marcus R. , Braren Rickmer F. , Herzig Stephan , Diaz Mauricio Berriel , Karampinos Dimitrios C.
Published: September 2021

Purpose: To evaluate the suitability of psoas and erector spinae muscle proton density fat fraction (PDFF) and fat volume as biomarkers for monitoring cachexia severity in an oncological cohort, and to evaluate regional variances in muscle parameters over time. Methods: In this prospective study, 58 oncological patients were examined by a 3 T MRI receiving between one and five scans. Muscle volume and PDFF were measured, segmentation masks were divided into proximal, middle and distal muscle section. Results: A regional variation of fat distribution in erector spinae muscle at baseline was found (p < 0.01). During follow-ups significant relative change of muscle parameters was observed. Relative maximum change of erector spinae muscle showed a significant regional variation. Correlation testing with age as a covariate revealed significant correlations for baseline psoas fat volume (r = −0.55, p < 0.01) and baseline psoas PDFF (r = −0.52, p = 0.02) with maximum BMI change during the course of the disease. Conclusion: In erector spinae muscles, a regional variation of fat distribution at baseline and relative maximum change of muscle parameters was observed. Our results indicate that psoas muscle PDFF and fat volume could serve as MRI-determined biomarkers for early risk stratification and disease monitoring regarding progression and severity of weight loss in cancer cachexia.
Cauliflower mosaic virus P6 Dysfunctions Histone Deacetylase HD2C to Promote Virus Infection
Authors: Li Shun , Lyu Shanwu , Liu Yujuan , Luo Ming , Shi Suhua , Deng Shulin
Published: September 2021

Histone deacetylases (HDACs) are vital epigenetic modifiers not only in regulating plant development but also in abiotic- and biotic-stress responses. Though to date, the functions of HD2C—an HD2-type HDAC—In plant development and abiotic stress have been intensively explored, its function in biotic stress remains unknown. In this study, we have identified HD2C as an interaction partner of the Cauliflower mosaic virus (CaMV) P6 protein. It functions as a positive regulator in defending against CaMV infection. The hd2c mutants show enhanced susceptibility to CaMV infection. In support, the accumulation of viral DNA, viral transcripts, and the deposition of histone acetylation on the viral minichromosomes are increased in hd2c mutants. P6 interferes with the interaction between HD2C and HDA6, and P6 overexpression lines have similar phenotypes with hd2c mutants. In further investigations, P6 overexpression lines, together with CaMV infection plants, are more sensitive to ABA and NaCl with a concomitant increasing expression of ABA/NaCl-regulated genes. Moreover, the global levels of histone acetylation are increased in P6 overexpression lines and CaMV infection plants. Collectively, our results suggest that P6 dysfunctions histone deacetylase HD2C by physical interaction to promote CaMV infection.
Apical Sperm Hook Morphology Is Linked to Sperm Swimming Performance and Sperm Aggregation in Peromyscus Mice
Authors: Hook Kristin A. , Wilke Lauren M. , Fisher Heidi S.
Published: September 2021

Mammals exhibit a tremendous amount of variation in sperm morphology and despite the acknowledgement of sperm structural diversity across taxa, its functional significance remains poorly understood. Of particular interest is the sperm of rodents. While most Eutherian mammal spermatozoa are relatively simple cells with round or paddle-shaped heads, rodent sperm are often more complex and, in many species, display a striking apical hook. The function of the sperm hook remains largely unknown, but it has been hypothesized to have evolved as an adaptation to inter-male sperm competition and thus has been implicated in increased swimming efficiency or in the formation of collective sperm movements. Here we empirically test these hypotheses within a single lineage of Peromyscus rodents, in which closely related species naturally vary in their mating systems, sperm head shapes, and propensity to form sperm aggregates of varying sizes. We performed sperm morphological analyses as well as in vitro analyses of sperm aggregation and motility to examine whether the sperm hook (i) morphologically varies across these species and (ii) associates with sperm competition, aggregation, or motility. We demonstrate inter-specific variation in the sperm hook and then show that hook width negatively associates with sperm aggregation and sperm swimming speed, signifying that larger hooks may be a hindrance to sperm movement within this group of mice. Finally, we confirmed that the sperm hook hinders motility within a subset of Peromyscus leucopus mice that spontaneously produced sperm with no or highly abnormal hooks. Taken together, our findings suggest that any adaptive value of the sperm hook is likely associated with a function other than inter-male sperm competition, such as interaction with ova or cumulous cells during fertilization, or migration through the complex female reproductive tract.
Schisandrae chinensis Fructus Extract Ameliorates Muscle Atrophy in Streptozotocin-Induced Diabetic Mice by Downregulation of the CREB-KLF15 and Autophagy–Lysosomal Pathways
Authors: Choi Ho-Jung , Yeon Myeong-Hoon , Jun Hee-Sook
Published: September 2021

Type 1 diabetes mellitus is an autoimmune disease caused by the destruction of pancreatic beta cells. Many patients with type 1 diabetes experience skeletal muscle wasting. Although the link between type 1 diabetes and muscle wasting is not clearly known, insulin insufficiency and hyperglycemia may contribute to decreased muscle mass. In this study, we investigated the therapeutic effect of the ethanolic extract of Schisandrae chinensis Fructus (SFe) on muscle wasting in streptozotocin (STZ)-induced diabetic mice. STZ-diabetic C57BL/6 mice (blood glucose level ≥300 mg/dL) were orally administered SFe (250 or 500 mg/kg/day) for 6 weeks. We observed that SFe administration did not change blood glucose levels but increased gastrocnemius muscle weight, cross-sectional area, and grip strength in STZ-induced diabetic mice. Administration of SFe (500 mg/kg) decreased the expression of atrophic factors, such as MuRF1 and atrogin-1, but did not alter the expression of muscle synthetic factors. Further studies showed that SFe administration decreased the expression of KLF15 and p-CREB, which are upstream molecules of atrophic factors. Examination of the expression of molecules involved in autophagy–lysosomal pathways (e.g., p62/SQSTM1, Atg7, Beclin-1, ULK-1, LC3-I, and LC3-II) revealed that SFe administration significantly decreased the expression of p62/SQSTM1, LC3-I, and LC3-II; however, no changes were observed in the expression of Atg7, Beclin-1, or ULK-1. Our results suggest that SFe ameliorated muscle wasting in STZ-induced diabetic mice by decreasing protein degradation via downregulation of the CREB-KLF15-mediated UPS system and the p62/SQSTM1-mediated autophagy–lysosomal pathway.
Role of TCTP in Cell Biological and Disease Processes
Authors: Bommer Ulrich-Axel , Kawakami Toshiaki
Published: September 2021

Translationally controlled tumor protein (TCTP), also referred to as histamine-releasing factor (HRF) or fortilin, is a multifunctional protein, expressed in essentially all eukaryotic organisms [...]
Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes
Authors: Gomez-Perosanz Marta , Fiyouzi Tara , Fernandez-Arquero Miguel , Sidney John , Sette Alessandro , Reinherz Ellis L. , Lafuente Esther M. , Reche Pedro A.
Published: September 2021

Human rhinovirus (RV) is the most common cause of upper respiratory infections and exacerbations of asthma. In this work, we selected 14 peptides (6 from RV A and 8 from RV C) encompassing potential CD4 T cell epitopes. Peptides were selected for being highly conserved in RV A and C serotypes and predicted to bind to multiple human leukocyte antigen class II (HLA II) molecules. We found positive T cell recall responses by interferon gamma (IFNγ)-ELISPOT assays to eight peptides, validating seven of them (three from RV A and four from RV C) as CD4 T cell epitopes through intracellular cytokine staining assays. Additionally, we verified their promiscuous binding to multiple HLA II molecules by quantitative binding assays. According to their experimental HLA II binding profile, the combination of all these seven epitopes could be recognized by >95% of the world population. We actually determined IFNγ responses to a pool encompassing these CD4 T cell epitopes by intracellular cytokine staining, finding positive responses in 29 out of 30 donors. The CD4 T cell epitopes identified in this study could be key to monitor RV infections and to develop peptide-based vaccines against most RV A and C serotypes.
Zinc Oxide Zinc Sulfate versus Zinc Oxide Eugenol as Pulp Chamber Filling Materials in Primary Molar Pulpotomies
Authors: Moskovitz Moti , Tickotsky Nili , Dassa Maayan , Fux-Noy Avia , Shmueli Aviv , Halperson Elinor , Ram Diana
Published: September 2021

The long-term effect of Zinc oxide zinc sulfate (Coltosol®) dressing material on pulpotomy success and tooth survival has not yet been studied. This study compared the success rates of Zinc oxide zinc sulfate and zinc oxide eugenol as coronal dressing materials post radicular pulp amputation in primary teeth pulpotomies. This study included healthy two- to ten-year-old children who had pulpotomies on primary molars between 2012 and 2018 at the Pediatric Dentistry Clinic of the School of Dental Medicine. Data were analyzed at several follow-ups of up to 60 months. Kaplan-Meier survival curves were used to estimate survival probabilities of Zinc oxide zinc sulfate versus zinc oxide eugenol. In the 107 children included in this study, 54 teeth were filled with Zinc oxide zinc sulfate and 53 were filled with zinc oxide eugenol. Follow-up ranged from 12.2 to 73.3 months. Overall survival of Coltosol® vs. IRM filled teeth was 87.1% and 79.3%, respectively. Overall survival probabilities for Coltosol®-filled teeth at 15.5, 24 and 45 months were 95%, 89.8% and 79.7%, respectively, while for IRM they were 93.7%, 83% and 67.7%, respectively. Treatment failure rates and type of treated teeth did not differ between boys and girls (p-value = 0.77 and 0.87, respectively). Zinc oxide zinc sulfate and zinc oxide eugenol exhibited comparable high long-term success rates of up to five years (p = 0.16).
Drought Coincided with, but Does Not Explain, Late Holocene Megafauna Extinctions in SW Madagascar
Authors: Hixon Sean W. , Curtis Jason H. , Brenner Mark , Douglass Kristina G. , Domic Alejandra I. , Culleton Brendan J. , Ivory Sarah J. , Kennett Douglas J.
Published: September 2021

Climate drying could have transformed ecosystems in southern Madagascar during recent millennia by contributing to the extinction of endemic megafauna. However, the extent of regional aridification during the past 2000 years is poorly known, as are the responses of endemic animals and economically important livestock to drying. We inferred ~1600 years of climate change around Lake Ranobe, SW Madagascar, using oxygen isotope analyses of monospecific freshwater ostracods (Bradleystrandesia cf. fuscata) and elemental analyses of lake core sediment. We inferred past changes in habitat and diet of introduced and extinct endemic megaherbivores using bone collagen stable isotope and 14C datasets (n = 63). Extinct pygmy hippos and multiple giant lemur species disappeared from the vicinity of Ranobe during a dry interval ~1000–700 cal yr BP, but the simultaneous appearance of introduced cattle, high charcoal concentrations, and other evidence of human activity confound inference of drought-driven extirpations. Unlike the endemic megafauna, relatively low collagen stable nitrogen isotope values among cattle suggest they survived dry intervals by exploiting patches of wet habitat. Although megafaunal extirpations coincided with drought in SW Madagascar, coupled data from bone and lake sediments do not support the hypothesis that extinct megafauna populations collapsed solely because of drought. Given that the reliance of livestock on mesic patches will become more important in the face of projected climate drying, we argue that sustainable conservation of spiny forests in SW Madagascar should support local livelihoods by ensuring that zebu have access to mesic habitat. Additionally, the current interactions between pastoralism and riparian habitats should be studied to help conserve the island’s biodiversity.
Diagnostic Value of Contrast-Enhanced Ultrasound for Evaluation of Transjugular Intrahepatic Portosystemic Shunt Perfusion
Authors: Marschner Constantin A. , Geyer Thomas , Froelich Matthias F. , Rübenthaler Johannes , Schwarze Vincent , Clevert Dirk-André
Published: September 2021

Background: In patients with liver cirrhosis, transjugular intrahepatic portosystemic shunt (TIPS) displays an effective method for treating portal hypertension. Main indications include refractory ascites and secondary prevention of esophageal bleeding. Color Doppler ultrasound (CDUS) plays a leading role in the follow-up management, whereas contrast-enhanced ultrasound (CEUS) is not routinely considered. We compared the efficacy of CEUS to CDUS and highlighted differences compared to findings of corresponding computed tomography (CT) and magnetic resonance imaging (MRI). (2) Methods: On a retrospective basis, 106 patients with CEUS examination after TIPS were included. The enrollment period was 12 years (between 2008 and 2020) and the age group ranged from 23.3 to 82.1 years. In addition, 92 CDUS, 43 CT and 58 MRI scans were evaluated for intermodal comparison. (3) Results: Intermodal analysis and comparison revealed a high level of concordance between CDUS, CT and MRI in the vast majority of cases. In comparison to CDUS, the correlation of the relevant findings was 92.5%, 95.3% for CT and 87.9% for MRI. In some cases, however, additional information was provided by CEUS (4) Conclusions: CEUS depicts a safe and effective imaging modality for follow-up after TIPS. In addition to CDUS, CEUS enables specific assessment of stent pathologies and stent dysfunction due to its capacity to dynamically visualize single microbubbles at high spatial and temporal resolution. Due to the low number of adverse events regarding the application of contrast agents, CEUS can be administered to a very broad patient population, thus avoiding additional radiation exposure compared to CT angiography in cases with divergent findings during follow-up.
Accuracy of Pretreatment Ultrasonography Assessment of Intra-Abdominal Spread in Epithelial Ovarian Cancer: A Prospective Study
Authors: Tomasińska Agnieszka , Stukan Maciej , Badocha Michał , Myszewska Aleksandra
Published: September 2021

The aim of this study was to test the accuracy of ultrasonography performed by gynecological oncologists for the preoperative assessment of epithelial ovarian cancer (EOC) spread in the pelvis and abdominal cavity. A prospective, observational cohort study was performed at a single tertiary cancer care unit. Patients with suspected EOC were recruited and underwent comprehensive transvaginal and abdominal ultrasonography performed by a gynecological oncologist. Sixteen intra-abdominal localizations and parameters were assessed using ultrasonography and compared with surgical-pathological status (reference standard). Sensitivity, specificity, positive and negative predictive values, and overall accuracy were calculated. Differences were analyzed using Fisher’s exact and chi-square tests. Ultimately, we included 132 patients (median age 62 years), of whom 67% were in stage IIIC–IVB and 72% had serous cancer. Overall prediction accuracies for the involvement of the omentum, small bowel mesentery root, and frozen pelvis, and detecting ascites were >90%. Detecting the involvement of the pelvis peritoneum, liver and spleen hilum, and rectosigmoid colon, and predictions of disease stage and residual disease had overall accuracies of 80–90%. The lowest accuracy was for involvement of the abdominal peritoneum (69%) and diaphragm peritoneum (right 71%; left 75%) and surgical complexity prediction (77%). Stratification of results by presence or absence of ascites revealed significantly higher specificity of ultrasonography (clinically meaningful) for assessments of the abdominal/pelvic peritoneum, spleen hilum, and rectum wall, if there were ascites. A gynecological oncologist, experienced in surgery and sonology, performing comprehensive ultrasonography on patients with EOC can accurately detect intraperitoneal lesions and recognize critical disease manifestations and predict stage, surgical complexity, and residual disease, which allow accurate qualification of patients for primary debulking surgery or neoadjuvant chemotherapy.
Perfusion Patterns of Peripheral Organizing Pneumonia (POP) Using Contrast-Enhanced Ultrasound (CEUS) and Their Correlation with Immunohistochemically Detected Vascularization Patterns
Authors: Safai Zadeh Ehsan , Westhoff Christina Carolin , Keber Corinna Ulrike , Trenker Corinna , Dietrich Christoph Frank , Alhyari Amjad , Mohr Charlotte Gabriele Luise , Görg Christian
Published: September 2021

Purpose: To describe the perfusion patterns of peripheral organizing pneumonia (POP) by contrast-enhanced ultrasound (CEUS) and their correlation with vascularization patterns (VPs) represented by immunohistochemical CD34 endothelial staining. Methods: From October 2006 until December 2020, 38 consecutive patients with histologically confirmed POPs were standardized-examined by CEUS. The time to enhancement (TE; classified as an early pulmonary-arterial [PA] pattern of enhancement vs. delayed bronchial-arterial [BA] pattern of enhancement), the extent of enhancement (EE; classified as marked or reduced), the homogeneity of enhancement (HE; classified as homogeneous or inhomogeneous), and the decrease of enhancement (DE; classified as rapid washout [<120s] or late washout [≥120s]) were evaluated retrospectively. Furthermore, tissue samples from the study patients were immunohistochemically stained with CD34 antibody. The presence of avascular areas (AAs) and the VPs were evaluated in all tissue samples. Results: The majority of POPs showed a BA pattern of enhancement (71.1%), an isoechoic marked enhancement (76.3%), and an inhomogeneous enhancement (81.6%). A rapid DE was observed in 50.0% of cases. On CD34 staining, all POPs had a chaotic VP, indicating BA neoangiogenesis. AAs (abscess, necrosis, hemorrhage) were identified in (41.9%) cases with an inhomogeneous enhancement on CEUS. Conclusion: On CEUS, POPs predominantly revealed a marked inhomogeneous BA pattern of enhancement with a rapid washout in 50% of cases. Furthermore, we demonstrated that the presence of a PA pattern of enhancement, found in 28.9% of POPs, did not exclude a BA neoangiogenesis as an important feature of chronic inflammatory and malignant processes.
Calculus and Digital Natives in Rendezvous: wxMaxima Impact
Authors: Karjanto Natanael
Published: September 2021

This article covers how a computer algebra system (CAS) wxMaxima can be explored for teaching single-variable and multivariable calculus to Korean digital natives. We present several examples where wxMaxima can handle calculus problems easily, not straightforwardly but still successfully with some human intervention, and unsuccessfully. By soliciting qualitative feedback on students’ experience in exploiting the CAS, we gathered a mixed reaction. Although some students commented positively, the majority seemed to be resistant to embracing a new technological tool.
Review on Energy Storage Systems in Microgrids
Authors: Georgious Ramy , Refaat Rovan , Garcia Jorge , Daoud Ahmed A.
Published: September 2021

Energy storage systems (ESSs) are gaining a lot of interest due to the trend of increasing the use of renewable energies. This paper reviews the different ESSs in power systems, especially microgrids showing their essential role in enhancing the performance of electrical systems. Therefore, The ESSs classified into various technologies as a function of the energy storage form and the main relevant technical parameters. In this review paper, the most common classifications are presented, summarized, and compared according to their characteristics. A specific interest in electrochemical ESSs, especially battery energy storage systems, focusing on their classifications due to their importance in the residential sector. Besides that, the benefits and drawbacks of Lithium-Ion (Li-Ion) batteries are discussed due to their significance. Finally, the environmental impact of these ESSs is discussed.
Adjusting the Block Interval in PoW Consensus by Block Interval Process Improvement
Authors: Kim Heesang , Kim Dohoon
Published: September 2021

Blockchain is not widely applied in various fields due to the critical issue of scalability as part of the blockchain trilemma. This issue arises during consensus among the nodes in a public blockchain. To address the issue of low scalability with proof-of-work (PoW) consensus, various methods have been proposed for transaction per second (TPS) improvement. However, no such methods include an improvement in the consensus step. Therefore, to improve PoW public blockchain scalability, it is important to shorten the time required for PoW consensus. This paper proposes a method for minimizing the block intervals that occur during consensus over a PoW blockchain network. A shortened block interval leads to an increase in the probability of three different attacks: selfish mining, double-spending, and eclipse attacks. According to an experiment using Ethereum, with a typical PoW blockchain, it is inevitable to provide rewards for stable block mining in competition between mining pools. To find an optimal block interval in the PoW consensus algorithm, we conducted a four-step experiment. The purpose of this experiment was to verify the difficulty level and issues with Mainnet security. Therefore, considering stale block mining rewards, an optimal block interval is proposed. The Ethereum TPS was improved by at least 200%. Given this finding, it is considered possible to achieve a similar improvement in a different PoW blockchain. On balance, even if the block interval is shorter than that of the PoW Mainnet, network security falls by only 1.21% in Testnet, even with a rise in the stale block rate, while performance is increased at up to 120 TPS, which is three times higher than that in Mainnet.
Broadband Dual-Polarized 2 × 2 MIMO Antenna for a 5G Wireless Communication System
Authors: Cha Junghoon , Leem Choon-Seong , Kim Ikhwan , Lee Hakyoung , Lee Hojun
Published: September 2021

In this study, we proposed an indoor broadband dual-polarized 2 × 2 MIMO (multiple-input and multiple-output) antenna having dimensions of 240 mm × 200 mm × 40 mm, for application in 5G wireless communication systems. The proposed antenna comprised two vertically polarized circular monopole antennas (CMAs), two horizontally polarized modified rectangular dipole antennas (MRDAs), and a ground plane. The distance between the two MRDAs (MRDA1 and MRDA2) was 70.5 mm and 109.5 mm in the horizontal (x-direction) and 109.5 mm vertical (y-direction) directions, respectively. Conversely, the distance between the two CMAs (CMA1 and CMA2) was 109.5 mm and 70.5 mm in the horizontal (x-direction) and vertical (y-direction) directions, respectively. While the CMAs achieved broadband characteristics owing to the optimal gap between the dielectric and the driven radiator using a parasitic element, the MRDAs achieved broadband owing to the optimal distance between the dipole antennas. The observations in this experiment confirmed that the proposed could operate in the 5G NR n46 (5.15–5.925 GHz), n47 (5.855–5.925 GHz), n77 (3.3–4.2 GHz), n78 (3.3–3.8 GHz), and the n79 (4.4–5 GHz) bands. Moreover, it exhibited a wide impedance bandwidth (dB magnitude of S11) of 101% in the 2.3–7 GHz frequency range, high isolation (dB magnitude of S21), low envelope coefficient correlation (ECC), gain of over 5 dB, and average radiation efficiency of 87.19%, which verified its suitability for application in sub-6 GHz 5G wireless communication systems.
Soil Microbial Functional Diversity under the Single-Season Influence of Traditional Forest Management in a Sessile Oak Forest of Central Europe
Authors: Kučera Aleš , Holík Ladislav , Rosíková Jana , Volařík Daniel , Kneifl Michal , Vichta Tomáš , Knott Robert , Friedl Michal , Uherková Barbora , Kadavý Jan
Published: September 2021

This one-year study focuses on the responses of a soil environment to the implementation of traditional forest management practices in oak–hornbeam stands with the following treatments: cut (C), cut + litter raking (CR), cut + grazing (CG), cut + litter raking + grazing (CRG) and control (Ctrl). The cut was conducted in 2018 through extremely heavy thinning. In autumn of 2017 and 2018, we sampled the soils, focusing on microbial functional diversity (FD) assessments using BIOLOG EcoplateTM. After one season, the FD was the highest in the Ctrl stand and the lowest in the CRG stand. Furthermore, we detected significant seasonal differences in soil reaction, nitrate nitrogen content, phosphatase activity and microbial biomass among the treatments. In particular, the Ctrl stand was defined via FD indices and biochemical and biological soil properties that contrasted mainly with those of the CRG stand defined by the content of mineral nitrogen forms. The soil properties did not differ substantially in the remaining treatments. Of the 31 carbon sources defining FD, 6 were treatment-specific (putrescine, L-arginine, L-serine, L-threonine, D-cellobiose and glycogen), while the remaining carbon sources mainly displayed either uniform high or low activity across the treatments.
Antioxidant and Antibacterial Properties of Norway Spruce (Picea abies H. Karst.) and Eastern Hemlock (Tsuga canadensis (L.) Carrière) Cone Extracts
Authors: Hofmann Tamás , Albert Levente , Németh László , Vršanská Martina , Schlosserová Nikola , Voběrková Stanislava , Visi-Rajczi Eszter
Published: September 2021

According to recent studies, Norway spruce cones and eastern hemlock cones possess exceptionally high antioxidant capacities; however, the antioxidant efficiency of extractives and their bioactive effects have yet to be investigated in detail. The present article utilizes state-of-the art analytical chemical methodology and chemometric evaluation to reveal cone extractives with the highest antioxidant potential, accounting for possible bioactive effects. Antioxidant properties were determined by the Ferric reducing antioxidant power (FRAP) assay and the Folin–Ciocalteu’s total phenol content (TPC) assay. Structure and relative polyphenol concentrations were determined using liquid chromatography/diode array detection/tandem mass spectrometry. The antibacterial testing was conducted using Staphylococcus aureus and Escherichia coli strains. Using correlation analysis and factor analysis, a scoring evaluation was implemented to determine and compare the antioxidant efficiency of extractives. In Norway spruce, piceatannol-O-hexoside and coumaric acid derivatives were found to be the most powerful antioxidants, while in eastern hemlock, kaempferol glycosides were the most powerful. The antibacterial test did not show any promising antimicrobial activity against E. coli, although a visible inhibitory effect on S. aureus strain was observed.
Monitoring of Carriageway Cross Section Profiles on Forest Roads: Assessment of an Ultrasound Data Based Road Scanner with TLS Data Reference
Authors: Starke Michael , Kunneke Anton , Ziesak Martin
Published: September 2021

Forest roads are an important element in forest management as they provide infrastructure for different forest stakeholder groups. Over time, a variety of road assessment concepts for better planning were initiated. The monitoring of the surface cross-section profile of forest roads particularly offers the possibility to take early action in restoring a road segment and avoiding higher future costs. One vehicle-based monitoring system that relies on ultrasound sensors addresses this topic. With advantages in its dirt influence tolerance and high temporal resolution, but shortcomings in horizontal and vertical measuring accuracy, the system was tested against high resolution terrestrial laser scanner (TLS) data to find and assess working scenarios that fit the low- resolution measuring principle. In a related field test, we found low correct road geometry interpretation rates of 54.3% but rising to 91.2% under distinctive geometric properties. The further applied line- and segment-based method used to transform the TLS data to fit the road scanner measuring method allows the transfer of the road scanner evaluation principle to point-cloud or raster data of different origins.