bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics and Influencing Factors of Spatiotemporal Distribution of Rural Houses Construction Development in Mountainous Villages of China (1980–2019): A Case Study of Qingyuan Town
Authors: Liu Naifei , Zhang Huinan , Yue Kaijian , Shan Jun
Published: June 2024

Rural house is a fundamental component of rural settlements, and understanding its construction and development characteristics is crucial for rural land use and development planning. This paper focuses on the spatiotemporal characteristics and influencing factors of Rural Houses Construction Development (RHCD) from 1980 to 2019 with a case study of Qingyuan Town in China. Based on the literature review and filed research, a set of evaluation indicators for RHCD was established. The article calculates RHCD indicators from temporal and spatial dimensions, uses the location entropy method to demonstrate the spatial distribution of indicators, and classifies the RHCD type of 14 villages in Qingyuan Town using clustering algorithms. It also analyzes the influencing factors of spatiotemporal distribution. The results show that the RHCD in Qingyuan Town exhibits typical characteristics of mountainous areas and aligns with the development trends of rural society in China. Population growth, geographical location, and economic development are the primary driving factors for the quantity indicator (Qi), while economic growth, construction technology, industrial development, and policy adjustments are the key factors influencing the form indicator (Fi). In future policy-making, greater emphasis should be placed on optimizing development strategies, improving data and monitoring systems, and integrating administrative strength with actual development needs.
Exploring Trichoderma Species in Industrial Wastewater: Morphological and Molecular Insights from Isolates
Authors: Bint-e-Zahira Syeda , Khalid Abdul Nasir , Yousaf Nousheen , Iqbal Muhammad , Anwar Tauseef , Qureshi Huma , Salmen Saleh H. , Ansari Mohammad Javed
Published: June 2024

The genus Trichoderma holds economic significance due to its widespread distribution and diverse applications, including biological control, enzyme production, and various biotechnological uses. The accurate identification of Trichoderma species is crucial given their close association with human activities. Despite previous efforts in classification, a comprehensive analysis combining morphological and molecular approaches is necessary. This study focuses on the isolation of four Trichoderma species from industrial wastewater in Pakistan, expanding on the known diversity in the region; isolation involved collecting samples from industrial wastewater effluents at specific sites in Punjab, Pakistan. Trichoderma strains were cultured and purified on solid media, with subsequent biomass production for bisorptional activity. Morphological characterization included colony features and microscopic examinations. DNA extraction, polymerase chain reaction (PCR), and sequencing of the internal transcribed spacer (ITS) region were conducted for molecular analysis. Phylogenetic analysis was performed using the Maximum Likelihood Algorithm. The study identified three Trichoderma species, viz. T. citrinoviride, T. erinaceum, and T. longibrachiatum. Each species was characterized morphologically and supported by molecular–phylogenetic analysis. Illustrations of microscopic features and a phylogenetic tree based on the ITS-nrDNA region were recorded. T. citrinoviride and T. longibrachiatum, isolated from steel mill and tanneries wastewater, respectively, were differentiated based on morphological characteristics such as phialides and conidia. The combination of morphological and molecular techniques enhances the accuracy of species identification. The study highlights the significance of Trichoderma in industrial wastewater environments and underscores the need for continued research in this area. Future research should focus on exploring the ecological roles and potential applications of the newly identified Trichoderma species. Additionally, further investigations into the biotechnological potential of these species, including enzyme production and bioremediation capabilities, would contribute to their practical applications.
A Review of Advances in Molecular Imaging of Rheumatoid Arthritis: From In Vitro to Clinic Applications Using Radiolabeled Targeting Vectors with Technetium-99m
Authors: Ali Muhammad , Benfante Viviana , Di Raimondo Domenico , Laudicella Riccardo , Tuttolomondo Antonino , Comelli Albert
Published: June 2024

Rheumatoid arthritis (RA) is a systemic autoimmune disorder caused by inflammation of cartilaginous diarthrodial joints that destroys joints and cartilage, resulting in synovitis and pannus formation. Timely detection and effective management of RA are pivotal for mitigating inflammatory arthritis consequences, potentially influencing disease progression. Nuclear medicine using radiolabeled targeted vectors presents a promising avenue for RA diagnosis and response to treatment assessment. Radiopharmaceutical such as technetium-99m (99mTc), combined with single photon emission computed tomography (SPECT) combined with CT (SPECT/CT), introduces a more refined diagnostic approach, enhancing accuracy through precise anatomical localization, representing a notable advancement in hybrid molecular imaging for RA evaluation. This comprehensive review discusses existing research, encompassing in vitro, in vivo, and clinical studies to explore the application of 99mTc radiolabeled targeting vectors with SPECT imaging for RA diagnosis. The purpose of this review is to highlight the potential of this strategy to enhance patient outcomes by improving the early detection and management of RA.