bluuebunny/crossref_metadata_2025 huggingface dataset:
Polarimetric Radar Observations of Biological Scatterers in the Eye of Typhoon Lekima (2019)
Authors: Huang Kehui , Huang Hao , Gu Tiecheng , Wang Bo , Lou Chengwu
Published: September 2023

Tropical cyclones not only cause strong winds and heavy rainfall, but they can also facilitate the transport of birds and insects from tropical regions to areas along their paths. Before super Typhoon Lekima made landfall in 2019, an operational polarimetric radar in Wenzhou City observed biological scatterers in the typhoon's eye. These scatterers were likely birds and insects that were trapped in the calm center of the typhoon by strong winds and heavy rain. The polarimetric variables of these biological scatterers had specific characteristics: low reflectivity factor (ZH) values with a median of 7.5 dBZ, low cross‐correlation coefficient (ρhv) values with a median of 0.65, large differential reflectivity (ZDR) values with a median of 2.8 dB and a maximum of ∼7.8 dB, and widely‐distributed differential phase (ΦDP) values with 25th and 75th percentiles ranging from 1.8° to 36.0° and a median of 20.1°. When the edge of the eye reached the coastline, the birds and insects landed, with the biological scatterer signature changing from a circular shape to a band shape. We further compared Lekima with Atlantic Hurricane Irene which happened in 2011, and both storms had similar polarimetric characteristics contributed by both birds and insects. However, the biological echoes in Lekima better exhibited the characteristics of birds with a larger proportion of samples with ZDR lower than 5 dB as well as large ΦDP and low ρhv values. This finding could help to understand the role of typhoons in driving biological migration between oceans and/or islands and continents.
Local Topography and Streambed Hydraulic Conductivity Influence Riparian Groundwater Age and Groundwater‐Surface Water Connection
Authors: Warix S. R. , Navarre‐Sitchler A. , Manning A. H. , Singha K.
Published: September 2023

The western U.S. is experiencing increasing rain to snow ratios due to climate change, and scientists are uncertain how changing recharge patterns will affect future groundwater‐surface water connection. We examined how watershed topography and streambed hydraulic conductivity impact groundwater age and stream discharge at eight sites along a headwater stream within the Manitou Experimental Forest, CO USA. To do so, we measured: (a) continuous stream and groundwater discharge/level and specific conductivity from April to November 2021; (b) biweekly stream and groundwater chemistry; (c) groundwater chlorofluorocarbons and tritium in spring and fall; (d) streambed hydraulic conductivity; and (e) local slope. We used the chemistry data to calculate fluorite saturation states that were used to inform end‐member mixing analysis of streamflow source. We then combined chlorofluorocarbon and tritium data to estimate the age composition of riparian groundwater. Our data suggest that future stream drying is more probable where local slope is steep and streambed hydraulic conductivity is high. In these areas, groundwater source shifted seasonally, as indicated by age increases, and we observed a high fraction of groundwater in streamflow, primarily interflow from adjacent hillslopes. In contrast, where local slope is flat and streambed hydraulic conductivity is low, streamflow is more likely to persist as groundwater age was seasonally constant and buffered by storage in alluvial sediments. Groundwater age and streamflow paired with characterization of watershed topography and subsurface characteristics enabled identification of likely controls on future stream drying patterns.
Grip strength positively correlates with blood pressure in individuals with abnormal adiposity
Authors: Pratt Jedd , Paolocci Nazareno , Boreham Colin , De Vito Giuseppe
Published: September 2023

Although strong positive correlations exist between grip strength and cardiovascular health, the association between grip strength and blood pressure (BP) is less clear. In this regard, a more precise relationship between grip strength and BP may be revealed by considering adiposity. We examined the association between grip strength and BP in 9424 individuals aged 18–92 years, while controlling for or stratifying by body mass index (BMI) or body fat (BF)%. Grip strength, BP and BF% were determined using dynamometry, sphygmomanometry and dual-energy x-ray absorptiometry. Overall, those with elevated BP had greater grip strength than those with normal BP (39.17 kg vs 38.38 kg, p < 0.001); however, following stratification this was only observed in overweight or obese individuals (42.08 kg vs 41.10 kg, p = 0.003 and 41.34 kg vs 40.03 kg, p = 0.033), and those within the highest BF% tertile (37.95 kg vs 36.52 kg, p < 0.001). Overall, higher grip strength was associated with an increased odds for elevated BP (OR = 1.014, 95% CI = 1.004–1.024, p = 0.004); however, after stratification the increased odds was only observed in overweight or obese individuals (OR = 1.025, 95% CI = 1.010–1.039, p < 0.001 and OR = 1.018, 95% CI = 1.004–1.031, p = 0.010), and those within the highest BF% tertile (OR = 1.036, 95% CI = 1.022–1.051, p < 0.001). Individuals with low grip strength and high BF% had lower odds for elevated BP (OR = 0.514, 95% CI = 0.341–0.775, p = 0.002), whereas those with low grip strength and low BF% had higher odds for elevated BP (OR = 2.162, 95% CI = 1.026–4.555, p = 0.043). Our findings show that higher grip strength is related to higher BP in overweight or obese individuals, or those with a high BF%. Having a BMI < 25 kg/m2 or lower BF% may neutralise this association.
FBXO21 mediated degradation of p85α regulates proliferation and survival of acute myeloid leukemia
Authors: Dobish Kasidy K. , Wittorf Karli J. , Swenson Samantha A. , Bean Dalton C. , Gavile Catherine M. , Woods Nicholas T. , Ghosal Gargi , Hyde R. Katherine , Buckley Shannon M.
Published: September 2023

Acute myeloid leukemia (AML) is a heterogeneous disease characterized by clonal expansion of myeloid blasts in the bone marrow (BM). Despite advances in therapy, the prognosis for AML patients remains poor, and there is a need to identify novel molecular pathways regulating tumor cell survival and proliferation. F-box ubiquitin E3 ligase, FBXO21, has low expression in AML, but expression correlates with survival in AML patients and patients with higher expression have poorer outcomes. Silencing FBXO21 in human-derived AML cell lines and primary patient samples leads to differentiation, inhibition of tumor progression, and sensitization to chemotherapy agents. Additionally, knockdown of FBXO21 leads to up-regulation of cytokine signaling pathways. Through a mass spectrometry-based proteomic analysis of FBXO21 in AML, we identified that FBXO21 ubiquitylates p85α, a regulatory subunit of the phosphoinositide 3-kinase (PI3K) pathway, for degradation resulting in decreased PI3K signaling, dimerization of free p85α and ERK activation. These findings reveal the ubiquitin E3 ligase, FBXO21, plays a critical role in regulating AML pathogenesis, specifically through alterations in PI3K via regulation of p85α protein stability.
Quantized resistance revealed at the criticality of the quantum anomalous Hall phase transitions
Authors: Deng Peng , Zhang Peng , Eckberg Christopher , Chong Su Kong , Yin Gen , Emmanouilidou Eve , Che Xiaoyu , Ni Ni , Wang Kang L.
Published: September 2023

In multilayered magnetic topological insulator structures, magnetization reversal processes can drive topological phase transitions between quantum anomalous Hall, axion insulator, and normal insulator states. Here we report an examination of the critical behavior of two such transitions: the quantum anomalous Hall to normal insulator (QAH-NI), and quantum anomalous Hall to axion insulator (QAH-AXI) transitions. By introducing a new analysis protocol wherein temperature dependent variations in the magnetic coercivity are accounted for, the critical behavior of the QAH-NI and QAH-AXI transitions are evaluated over a wide range of temperature and magnetic field. Despite the uniqueness of these different transitions, quantized longitudinal resistance and Hall conductance are observed at criticality in both cases. Furthermore, critical exponents were extracted for QAH-AXI transitions occurring at magnetization reversals of two different magnetic layers. The observation of consistent critical exponents and resistances in each case, independent of the magnetic layer details, demonstrates critical behaviors in quantum anomalous Hall transitions to be of electronic rather than magnetic origin. Our finding offers a new avenue for studies of phase transition and criticality in QAH insulators.
The relationship between geographic range size and rates of species diversification
Authors: Smyčka Jan , Toszogyova Anna , Storch David
Published: September 2023

Range size is a universal characteristic of every biological species, and is often assumed to affect diversification rate. There are strong theoretical arguments that large-ranged species should have higher rates of diversification. On the other hand, the observation that small-ranged species are often phylogenetically clustered might indicate high diversification of small-ranged species. This discrepancy between theory and the data may be caused by the fact that typical methods of data analysis do not account for range size changes during speciation. Here we use a cladogenetic state-dependent diversification model applied to mammals to show that range size changes during speciation are ubiquitous and small-ranged species indeed diversify generally slower, as theoretically expected. However, both range size and diversification are strongly influenced by idiosyncratic and spatially localized events, such as colonization of an archipelago or a mountain system, which often override the general pattern of range size evolution.
A Bischler-Napieralski and homo-Mannich sequence enables diversified syntheses of sarpagine alkaloids and analogues
Authors: Qiu Hanyue , Fei Xinghai , Yang Jiaojiao , Qiao Zhen , Yuan Shan , Zhang Hu , He Ling , Zhang Min
Published: September 2023

Sarpagine alkaloids offer signicant opportunities in drug discovery, yet the efficient total syntheses and diverse structural modifications of these natural products remain highly challenging due to the architectural complexity. Here we show a homo-Mannich reaction of cyclopropanol with imines generated via a Bischler-Napieralski reaction enables a protecting-group-free, redox economic, four-step access to the tetracyclic sarpagine core from L-tryptophan esters. Based on this advancement, diversified syntheses of sarpagine alkaloids and analogues are achieved in a short synthetic route. The systematic anticancer evaluation indicates that natural products vellosimine and Na-methyl vellosimine possess modest anticancer activity. Intensive structural optimization of these lead molecules and exploration of the structure−activity relationship lead to the identification of analogue 15ai with an allene unit showing a tenfold improvement in anticancer activities. Further mechanism studies indicate compound 15ai exertes antiproliferation effects by inducing ferroptosis, which is an appealing non-apoptotic cell death form that may provide new solutions in future cancer therapies.
An anisotropic van der Waals dielectric for symmetry engineering in functionalized heterointerfaces
Authors: Li Zeya , Huang Junwei , Zhou Ling , Xu Zian , Qin Feng , Chen Peng , Sun Xiaojun , Liu Gan , Sui Chengqi , Qiu Caiyu , Lu Yangfan , Gou Huiyang , Xi Xiaoxiang , Ideue Toshiya , Tang Peizhe , Iwasa Yoshihiro , Yuan Hongtao
Published: September 2023

Van der Waals dielectrics are fundamental materials for condensed matter physics and advanced electronic applications. Most dielectrics host isotropic structures in crystalline or amorphous forms, and only a few studies have considered the role of anisotropic crystal symmetry in dielectrics as a delicate way to tune electronic properties of channel materials. Here, we demonstrate a layered anisotropic dielectric, SiP2, with non-symmorphic twofold-rotational C2 symmetry as a gate medium which can break the original threefold-rotational C3 symmetry of MoS2 to achieve unexpected linearly-polarized photoluminescence and anisotropic second harmonic generation at SiP2/MoS2 interfaces. In contrast to the isotropic behavior of pristine MoS2, a large conductance anisotropy with an anisotropy index up to 1000 can be achieved and modulated in SiP2-gated MoS2 transistors. Theoretical calculations reveal that the anisotropic moiré potential at such interfaces is responsible for the giant anisotropic conductance and optical response. Our results provide a strategy for generating exotic functionalities at dielectric/semiconductor interfaces via symmetry engineering.
NEUROD1 reinforces endocrine cell fate acquisition in pancreatic development
Authors: Bohuslavova Romana , Fabriciova Valeria , Smolik Ondrej , Lebrón-Mora Laura , Abaffy Pavel , Benesova Sarka , Zucha Daniel , Valihrach Lukas , Berkova Zuzana , Saudek Frantisek , Pavlinkova Gabriela
Published: September 2023

NEUROD1 is a transcription factor that helps maintain a mature phenotype of pancreatic β cells. Disruption ofNeurod1during pancreatic development causes severe neonatal diabetes; however, the exact role of NEUROD1 in the differentiation programs of endocrine cells is unknown. Here, we report a crucial role of the NEUROD1 regulatory network in endocrine lineage commitment and differentiation. Mechanistically, transcriptome and chromatin landscape analyses demonstrate thatNeurod1inactivation triggers a downregulation of endocrine differentiation transcription factors and upregulation of non-endocrine genes within theNeurod1-deficient endocrine cell population, disturbing endocrine identity acquisition.Neurod1deficiency altered the H3K27me3 histone modification pattern in promoter regions of differentially expressed genes, which resulted in gene regulatory network changes in the differentiation pathway of endocrine cells, compromising endocrine cell potential, differentiation, and functional properties.
High-dose alkylating chemotherapy in BRCA-altered triple-negative breast cancer: the randomized phase III NeoTN trial
Authors: Vliek Sonja , Hilbers Florentine S. , van Werkhoven Erik , Mandjes Ingrid , Kessels Rob , Kleiterp Sieta , Lips Esther H. , Mulder Lennart , Kayembe Mutamba T. , Loo Claudette E. , Russell Nicola S. , Vrancken Peeters Marie-Jeanne T. F. D. , Holtkamp Marjo J. , Schot Margaret , Baars Joke W. , Honkoop Aafke H. , Vulink Annelie J. E. , Imholz Alex L. T. , Vrijaldenhoven Suzan , van den Berkmortel Franchette W. P. J. , Meerum Terwogt Jetske M. , Schrama Jolanda G. , Kuijer Philomeen , Kroep Judith R. , van der Padt-Pruijsten Annemieke , Wesseling Jelle , Sonke Gabe S. , Gilhuijs Kenneth G. A. , Jager Agnes , Nederlof Petra , Linn Sabine C.
Published: September 2023

Exploratory analyses of high-dose alkylating chemotherapy trials have suggested that BRCA1 or BRCA2-pathway altered (BRCA-altered) breast cancer might be particularly sensitive to this type of treatment. In this study, patients with BRCA-altered tumors who had received three initial courses of dose-dense doxorubicin and cyclophosphamide (ddAC), were randomized between a fourth ddAC course followed by high-dose carboplatin-thiotepa-cyclophosphamide or conventional chemotherapy (initially ddAC only or ddAC-capecitabine/decetaxel [CD] depending on MRI response, after amendment ddAC-carboplatin/paclitaxel [CP] for everyone). The primary endpoint was the neoadjuvant response index (NRI). Secondary endpoints included recurrence-free survival (RFS) and overall survival (OS). In total, 122 patients were randomized. No difference in NRI-score distribution (p = 0.41) was found. A statistically non-significant RFS difference was found (HR 0.54; 95% CI 0.23–1.25; p = 0.15). Exploratory RFS analyses showed benefit in stage III (n = 35; HR 0.16; 95% CI 0.03–0.75), but not stage II (n = 86; HR 1.00; 95% CI 0.30–3.30) patients. For stage III, 4-year RFS was 46% (95% CI 24–87%), 71% (95% CI 48–100%) and 88% (95% CI 74–100%), for ddAC/ddAC-CD, ddAC-CP and high-dose chemotherapy, respectively. No significant differences were found between high-dose and conventional chemotherapy in stage II-III, triple-negative, BRCA-altered breast cancer patients. Further research is needed to establish if there are patients with stage III, triple negative BRCA-altered breast cancer for whom outcomes can be improved with high-dose alkylating chemotherapy or whether the current standard neoadjuvant therapy including carboplatin and an immune checkpoint inhibitor is sufficient. Trial Registration: NCT01057069.
Opposing roles for TGFβ- and BMP-signaling during nascent alveolar differentiation in the developing human lung
Authors: Frum Tristan , Hsu Peggy P. , Hein Renee F. C. , Conchola Ansley S. , Zhang Charles J. , Utter Olivia R. , Anand Abhinav , Zhang Yi , Clark Sydney G. , Glass Ian , Sexton Jonathan Z. , Spence Jason R.
Published: September 2023

Alveolar type 2 (AT2) cells function as stem cells in the adult lung and aid in repair after injury. The current study aimed to understand the signaling events that control differentiation of this therapeutically relevant cell type during human development. Using lung explant and organoid models, we identified opposing effects of TGFβ- and BMP-signaling, where inhibition of TGFβ- and activation of BMP-signaling in the context of high WNT- and FGF-signaling efficiently differentiated early lung progenitors into AT2-like cells in vitro. AT2-like cells differentiated in this manner exhibit surfactant processing and secretion capabilities, and long-term commitment to a mature AT2 phenotype when expanded in media optimized for primary AT2 culture. Comparing AT2-like cells differentiated with TGFβ-inhibition and BMP-activation to alternative differentiation approaches revealed improved specificity to the AT2 lineage and reduced off-target cell types. These findings reveal opposing roles for TGFβ- and BMP-signaling in AT2 differentiation and provide a new strategy to generate a therapeutically relevant cell type in vitro.
Nutrient footprint versus EPA + DHA security in land-locked regions—more of local pond farmed, imported marine fish or fish oil capsules?
Authors: Roy Koushik , Dvorak Petr , Machova Zdenka , Mraz Jan
Published: September 2023

EPA + DHA intake in land-locked central Europe (CE) is barely fulfilled. Imported marine fish/farmed salmonids are likely the backbone of an ailing EPA + DHA security. Supplementing with captured marine fish oil capsules (~0.5 g up to 1.6 g CO2-eq. mg EPA + DHA−1) could be comparable in GHG emissions with fish consumption itself (~1 g to as low as 0.6 g CO2-eq. mg EPA + DHA−1). But synergistic benefits of EPA + DHA intake by consuming fish protein need consideration too. Taking semi-intensive pond carp and intensively farmed salmon as models, we analyzed footprint, eco-services, and resource use efficiency perspectives of achieving EPA + DHA security in a CE region. Despite a lower production footprint, pond-farmed fish greatly lag in EPA + DHA supply (carp 101–181 mg 100 g−1 < salmon 750–1300 mg 100 g−1). It doubles-to-quadruples footprint ‘per mg’ of EPA + DHA: nitrogen (carp 18.3 > salmon 8.7 mg N), phosphorus (carp 6.8 > salmon 1.6 mg P), and climate change (carp 1.84 > salmon 0.8 g CO2-eq.). With enhancements in pond carp (>300 mg EPA + DHA 100 g−1), these differences may cease to exist. Harnessing EPA + DHA bioaccumulation pathways active in ponds, finishing feeding strategies, and polyculture, the EPA + DHA content in pond fish may be increased. Ecosystem services with EPA + DHA mining from pond food web or high EPA + DHA output-to-input ratio (pond carp 1–200 > RAS salmon 0.75) make ponds an eco-efficient system. As fish consumption in CE must improve, pond-farmed fish would be needed to complement (but not substitute) salmonid/marine fish/oil capsules consumption. Achieving EPA + DHA security with minimum pressure on the environment or global resources.
One high quality genome and two transcriptome datasets for new species of Mantamonas, a deep-branching eukaryote clade
Authors: Blaz Jazmin , Galindo Luis Javier , Heiss Aaron A. , Kaur Harpreet , Torruella Guifré , Yang Ashley , Alexa Thompson L. , Filbert Alexander , Warring Sally , Narechania Apurva , Shiratori Takashi , Ishida Ken-ichiro , Dacks Joel B. , López-García Purificación , Moreira David , Kim Eunsoo , Eme Laura
Published: September 2023

Mantamonads were long considered to represent an “orphan” lineage in the tree of eukaryotes, likely branching near the most frequently assumed position for the root of eukaryotes. Recent phylogenomic analyses have placed them as part of the “CRuMs” supergroup, along with collodictyonids and rigifilids. This supergroup appears to branch at the base of Amorphea, making it of special importance for understanding the deep evolutionary history of eukaryotes. However, the lack of representative species and complete genomic data associated with them has hampered the investigation of their biology and evolution. Here, we isolated and described two new species of mantamonads, Mantamonas vickermani sp. nov. and Mantamonas sphyraenae sp. nov., for each of which we generated transcriptomic sequence data, as well as a high-quality genome for the latter. The estimated size of the M. sphyraenae genome is 25 Mb; our de novo assembly appears to be highly contiguous and complete with 9,416 predicted protein-coding genes. This near-chromosome-scale genome assembly is the first described for the CRuMs supergroup.
Reservoir inventory for China in 2016 and 2021
Authors: Zhang Haiying , Chen Fang , Wang Lei , Wang Ning , Yu Bo
Published: September 2023

Reservoir inventories are essential for investigating the impact of climate change and anthropogenic activities on water scape changes. They provide fundamental data sources to explore the sustainability and management efficiency of water resources. However, publicly released reservoir inventories are currently limited to a single temporal domain. As a result, the effectiveness of governmental policy implementation on water resources remains to be explored due to the lack of multi-time datasets. In this study, we generated a reservoir inventory for China for the years 2016 and 2021 with an overall accuracy of 99.71%. The reservoirs were visually interpreted from annually composited Landsat images, and each reservoir is represented by a polygon with attributes of reservoir name, area and storage capacity. About 10.32% of the reservoirs have increased storage capacity from 2016 to 2021, while 22.73% have decreased. Most provinces and river basins in China have expanded their accumulated storage capacity from 2016 to 2021.
TIHM: An open dataset for remote healthcare monitoring in dementia
Authors: Palermo Francesca , Chen Yu , Capstick Alexander , Fletcher-Loyd Nan , Walsh Chloe , Kouchaki Samaneh , True Jessica , Balazikova Olga , Soreq Eyal , Scott Gregory , Rostill Helen , Nilforooshan Ramin , Barnaghi Payam
Published: September 2023

Dementia is a progressive condition that affects cognitive and functional abilities. There is a need for reliable and continuous health monitoring of People Living with Dementia (PLWD) to improve their quality of life and support their independent living. Healthcare services often focus on addressing and treating already established health conditions that affect PLWD. Managing these conditions continuously can inform better decision-making earlier for higher-quality care management for PLWD. The Technology Integrated Health Management (TIHM) project developed a new digital platform to routinely collect longitudinal, observational, and measurement data, within the home and apply machine learning and analytical models for the detection and prediction of adverse health events affecting the well-being of PLWD. This work describes the TIHM dataset collected during the second phase (i.e., feasibility study) of the TIHM project. The data was collected from homes of 56 PLWD and associated with events and clinical observations (daily activity, physiological monitoring, and labels for health-related conditions). The study recorded an average of 50 days of data per participant, totalling 2803 days.
Impact of fractionated cisplatin and radiation treatment on cell growth and accumulation of DNA damage in two normal cell types differing in origin
Authors: Akuwudike Pamela , López-Riego Milagrosa , Dehours Cloé , Lundholm Lovisa , Wojcik Andrzej
Published: September 2023

Evidence on the impact of chemotherapy on radiotherapy-induced second malignant neoplasms is controversial. We estimated how cisplatin modulates the in vitro response of two normal cell types to fractionated radiation. AHH-1 lymphoblasts and VH10 fibroblasts were irradiated at 1 Gy/fraction 5 and 3 times per week during 12 and 19 days, respectively, and simultaneously treated with 0.1, 0.2, 0.4, 0.8, 1.7 and 3.3 µM of cisplatin twice a week. Cell growth during treatment was monitored. Cell growth/cell death and endpoints related to accumulation of DNA damage and, thus, carcinogenesis, were studied up to 21 days post treatment in cells exposed to radiation and the lowest cisplatin doses. Radiation alone significantly reduced cell growth. The impact of cisplatin alone below 3.3 µM was minimal. Except the lowest dose of cisplatin in VH10 cells, cisplatin reduced the inhibitory effect of radiation on cell growth. Delayed cell death was highest in the combination groups while the accumulation of DNA damage did not reveal a clear pattern. In conclusion, fractionated, concomitant exposure to radiation and cisplatin reduces the inhibitory effect of radiation on cell proliferation of normal cells and does not potentiate delayed effects resulting from accumulation of DNA damage.
Learning and predicting the unknown class using evidential deep learning
Authors: Nagahama Akihito
Published: September 2023

In practical deep-learning applications, such as medical image analysis, autonomous driving, and traffic simulation, the uncertainty of a classification model’s output is critical. Evidential deep learning (EDL) can output this uncertainty for the prediction; however, its accuracy depends on a user-defined threshold, and it cannot handle training data with unknown classes that are unexpectedly contaminated or deliberately mixed for better classification of unknown class. To address these limitations, I propose a classification method called modified-EDL that extends classical EDL such that it outputs a prediction, i.e. an input belongs to a collective unknown class along with a probability. Although other methods handle unknown classes by creating new unknown classes and attempting to learn each class efficiently, the proposed m-EDL outputs, in a natural way, the “uncertainty of the prediction” of classical EDL and uses the output as the probability of an unknown class. Although classical EDL can also classify both known and unknown classes, experiments on three datasets from different domains demonstrated that m-EDL outperformed EDL on known classes when there were instances of unknown classes. Moreover, extensive experiments under different conditions established that m-EDL can predict unknown classes even when the unknown classes in the training and test data have different properties. If unknown class data are to be mixed intentionally during training to increase the discrimination accuracy of unknown classes, it is necessary to mix such data that the characteristics of the mixed data are as close as possible to those of known class data. This ability extends the range of practical applications that can benefit from deep learning-based classification and prediction models.
Evaluation of a polarization-enhanced laparoscopy prototype for improved intra-operative visualization of peritoneal metastases
Authors: Schnelldorfer Thomas , Gnanatheepam Einstein , Trout Robert , Gado Ahmed , Pelletier Joyce-Ellen , Dinh Long T. , Hunter Martin , Georgakoudi Irene
Published: September 2023

Despite careful staging, the accuracy for preoperative detection of small distant metastases remains poor, creating a clinical need for enhanced operative staging to detect occult peritoneal metastases. This study evaluates a polarization-enhanced laparoscopy (PEL) prototype and assesses its potential for label-free contrast enhancement of peritoneal metastases. This is a first-in-human feasibility study, including 10 adult patients who underwent standard staging laparoscopy (SSL) for gastrointestinal malignancy along with PEL. Image frames of all detectable peritoneal lesions underwent analysis. Using Monte Carlo simulations, contrast enhancement based on the color dependence of PEL (mPEL) was assessed. The prototype performed safely, yet with limitations in illumination, fogging of the distal window, and image co-registration. Sixty-five lesions (56 presumed benign and 9 presumed malignant) from 3 patients represented the study sample. While most lesions were visible under human examination of both SSL and PEL videos, more lesions were apparent using SSL. However, this was likely due to reduced illumination under PEL. When controlling for such effects through direct comparisons of integrated (WLL) vs differential (PEL) polarization laparoscopy images, we found that PEL imaging yielded an over twofold Weber contrast enhancement over WLL. Further, enhancements in the discrimination between malignant and benign lesions were achieved by exploiting the PEL color contrast to enhance sensitivity to tissue scattering, influenced primarily by collagen. In conclusion, PEL appears safe and easy to integrate into the operating room. When controlling for the degree of illumination, image analysis suggested a potential for mPEL to provide improved visualization of metastases.
Rodents as vehicle for delivery of transgenic bacteria to make paratransgenic sand fly vectors of cutaneous leishmaniasis in field condition
Authors: Ghassemi Marzieh , Akhavan Amir Ahmad , Zahraei-Ramazani Alireza , Yakhchali Bagher , Arandian Mohammad Hossein , Jafari Reza , Akhlaghi Maryam , Shirani-Bidabadi Leila , Azam Kamal , Koosha Mona , Oshaghi Mohammad Ali
Published: September 2023

Vector-borne diseases, among them leishmaniasis, cause more than 700,000 deaths annually. The lack of an effective vaccination and the increasing resistance of sand flies to insecticides require the urgent development of innovative approaches to contain the disease. The use of engineered bacteria that express anti-parasite molecules (paratransgenesis) shows much promise. However, a challenge for implementation of this strategy is to devise means to introduce modified bacteria into sand flies in the field. In this study, we use rodent food bait as a delivery strategy to introduce two mCherry-fluorescent bacteria, Serratia AS1 and Enterobacter cloacae, into adult sand flies in field settings. Bacteria-infected food was provided to Rhombomys opimus rodents. These bacteria transiently pass through the rodent alimentary tract and are delivered to larval habitats with the rodent feces. The feces are ingested by sand fly larvae and, in the case of Serratia AS1, are trans-stadially transmitted to adults. This is the first report of targeting delivery of Serratia AS1 in a paratransgenic system to control transmission of leishmaniasis under field condition. This novel strategy shows promise for delivering transgenic bacteria to Leishmania vectors in the field.
Multiscale relevance of natural images
Authors: Lakhal Samy , Darmon Alexandre , Mastromatteo Iacopo , Marsili Matteo , Benzaquen Michael
Published: September 2023

We use an agnostic information-theoretic approach to investigate the statistical properties of natural images. We introduce the Multiscale Relevance (MSR) measure to assess the robustness of images to compression at all scales. Starting in a controlled environment, we characterize the MSR of synthetic random textures as function of image roughness $$\text{ H }$$

H

and other relevant parameters. We then extend the analysis to natural images and find striking similarities with critical ($$\text {H}\approx 0$$

H
≈
0

) random textures. We show that the MSR is more robust and informative of image content than classical methods such as power spectrum analysis. Finally, we confront the MSR to classical measures for the calibration of common procedures such as color mapping and denoising. Overall, the MSR approach appears to be a good candidate for advanced image analysis and image processing, while providing a good level of physical interpretability.
Epidemiological and molecular characterization of HBV and HCV infections in HIV-1-infected inmate population in Italy: a 2017–2019 multicenter cross-sectional study
Authors: Maggiorella Maria Teresa , Sernicola L. , Picconi O. , Pizzi E. , Belli R. , Fulgenzi D. , Rovetto C. , Bruni R. , Costantino A. , Taffon S. , Chionne P. , Madonna E. , Pisani G. , Borsetti A. , Falvino C. , Ranieri R. , Baccalini R. , Pansera A. , Castelvedere F. , Babudieri S. , Madeddu G. , Starnini G. , Dell’Isola S. , Cervellini P. , Ciccaglione A. R. , Ensoli B. , Buttò S.
Published: September 2023

HBV/HCV co-infection is common in HIV-1-infected prisoners. To investigate the characteristics of HIV co-infections, and to evaluate the molecular heterogeneity of HIV, HBV and HCV in prisoners, we carried-out a multicenter cross-sectional study, including 65 HIV-1-infected inmates enrolled in 5 Italian detention centers during the period 2017–2019. HIV-1 subtyping showed that 77.1% of inmates were infected with B subtype and 22.9% with non-B subtypes. Italian nationals were all infected with subtype B (93.1%), except two individuals, one infected with the recombinant form CRF72_BF1, and the other with the HIV-1 sub-subtype A6, both previously not identified in inmates of Italian nationality. Non-Italian nationals were infected with subtype B (52.6%), CRFs (36.8%) and sub-subtypes A1 and A3 (5.2%). HIV variants carrying resistance mutations to NRTI, NNRTI, PI and InSTI were found in 7 inmates, 4 of which were never exposed to the relevant classes of drugs associated with these mutations. HBV and/or HCV co-infections markers were found in 49/65 (75.4%) inmates, while 27/65 (41.5%) showed markers of both HBV and HCV coinfection. Further, Italian nationals showed a significant higher presence of HCV markers as compared to non-Italian nationals (p = 0.0001). Finally, HCV phylogenetic analysis performed in 18 inmates revealed the presence of HCV subtypes 1a, 3a, 4d (66.6%, 16.7% and 16.7%, respectively). Our data suggest the need to monitor HIV, HBV and HCV infections in prisons in order to prevent spreading of these viruses both in jails and in the general population, and to implement effective public health programs that limit the circulation of different genetic forms as well as of viral variants with mutations conferring resistance to treatment.
Genome-wide DNA methylation patterns in bumble bee (Bombus vosnesenskii) populations from spatial-environmental range extremes
Authors: Rahman Sarthok Rasique , Lozier Jeffrey D.
Published: September 2023

Unraveling molecular mechanisms of adaptation to complex environments is crucial to understanding tolerance of abiotic pressures and responses to climatic change. Epigenetic variation is increasingly recognized as a mechanism that can facilitate rapid responses to changing environmental cues. To investigate variation in genetic and epigenetic diversity at spatial and thermal extremes, we use whole genome and methylome sequencing to generate a high-resolution map of DNA methylation in the bumble bee Bombus vosnesenskii. We sample two populations representing spatial and environmental range extremes (a warm southern low-elevation site and a cold northern high-elevation site) previously shown to exhibit differences in thermal tolerance and determine positions in the genome that are consistently and variably methylated across samples. Bisulfite sequencing reveals methylation characteristics similar to other arthropods, with low global CpG methylation but high methylation concentrated in gene bodies and in genome regions with low nucleotide diversity. Differentially methylated sites (n = 2066) were largely hypomethylated in the northern high-elevation population but not related to local sequence differentiation. The concentration of methylated and differentially methylated sites in exons and putative promoter regions suggests a possible role in gene regulation, and this high-resolution analysis of intraspecific epigenetic variation in wild Bombus suggests that the function of methylation in niche adaptation would be worth further investigation.
Inducing an LCST in hydrophilic polysaccharides via engineered macromolecular hydrophobicity
Authors: Bubli Saniya Yesmin , Smolag Matthew , Blackwell Ellen , Lin Yung-Chun , Tsavalas John G. , Li Linqing
Published: September 2023

Thermoresponsive polysaccharide-based materials with tunable transition temperatures regulating phase-separated microdomains offer substantial opportunities in tissue engineering and biomedical applications. To develop novel synthetic thermoresponsive polysaccharides, we employed versatile chemical routes to attach hydrophobic adducts to the backbone of hydrophilic dextran and gradually increased the hydrophobicity of the dextran chains to engineer phase separation. Conjugating methacrylate moieties to the dextran backbone yielded a continuous increase in macromolecular hydrophobicity that induced a reversible phase transition whose lower critical solution temperature can be modulated via variations in polysaccharide concentration, molecular weight, degree of methacrylation, ionic strength, surfactant, urea and Hofmeister salts. The phase separation is driven by increased hydrophobic interactions of methacrylate residues, where the addition of surfactant and urea disassociates hydrophobic interactions and eliminates phase transition. Morphological characterization of phase-separated dextran solutions via scanning electron and flow imaging microscopy revealed the formation of microdomains upon phase transition. These novel thermoresponsive dextrans exhibited promising cytocompatibility in cell culture where the phase transition exerted negligible effects on the attachment, spreading and proliferation of human dermal fibroblasts. Leveraging the conjugated methacrylate groups, we employed photo-initiated radical polymerization to generate phase-separated hydrogels with distinct microdomains. Our bottom-up approach to engineering macromolecular hydrophobicity of conventional hydrophilic, non-phase separating dextrans to induce robust phase transition and generate thermoresponsive phase-separated biomaterials will find applications in mechanobiology, tissue repair and regenerative medicine.
Consistency of spectral results in cardiac dual-source photon-counting CT
Authors: Liu Leening P. , Shapira Nadav , Sahbaee Pooyan , Gang Grace J. , Knollman Friedrich D. , Chen Marcus Y. , Litt Harold I. , Noël Peter B.
Published: September 2023

We evaluate stability of spectral results at different heart rates, acquisition modes, and cardiac phases in first-generation clinical dual-source photon-counting CT (PCCT). A cardiac motion simulator with a coronary stenosis mimicking a 50% eccentric calcium plaque was scanned at five different heart rates (0, 60–100 bpm) with the three available cardiac scan modes (high pitch prospectively ECG-triggered spiral, prospectively ECG-triggered axial, retrospectively ECG-gated spiral). Subsequently, full width half max (FWHM) of the stenosis, Dice score (DSC) for the stenosed region, and eccentricity of the non-stenosed region were calculated for virtual monoenergetic images (VMI) at 50, 70, and 150 keV and iodine density maps at both diastole and systole. FWHM averaged differences of − 0.20, − 0.28, and − 0.15 mm relative to static FWHM at VMI 150 keV across acquisition parameters for high pitch prospectively ECG-triggered spiral, prospectively ECG-triggered axial, and retrospectively ECG-gated spiral scans, respectively. Additionally, there was no effect of heart rate and acquisition mode on FWHM at diastole (p-values < 0.001). DSC demonstrated similarity among parameters with standard deviations of 0.08, 0.09, 0.11, and 0.08 for VMI 50, 70, and 150 keV, and iodine density maps, respectively, with insignificant differences at diastole (p-values < 0.01). Similarly, eccentricity illustrated small differences across heart rate and acquisition mode for each spectral result. Consistency of spectral results at different heart rates and acquisition modes for different cardiac phase demonstrates the added benefit of spectral results from PCCT to dual-source CT to further increase confidence in quantification and advance cardiovascular diagnostics.
In vitro hyperspectral biomarkers of human chondrosarcoma cells in nanoparticle-mediated radiosensitization using carbon ions
Authors: Tudor Mihaela , Popescu Roxana Cristina , Negoita Raluca D. , Gilbert Antoine , Ilisanu Mihaela A. , Temelie Mihaela , Dinischiotu Anca , Chevalier François , Mihailescu Mona , Savu Diana Iulia
Published: September 2023

New therapeutic approaches are needed for the management of the highly chemo- and radioresistant chondrosarcoma (CHS). In this work, we used polyethylene glycol-encapsulated iron oxide nanoparticles for the intracellular delivery of the chemotherapeutic doxorubicin (IONPDOX) to augment the cytotoxic effects of carbon ions in comparison to photon radiation therapy. The in vitro biological effects were investigated in SW1353 chondrosarcoma cells focusing on the following parameters: cell survival using clonogenic test, detection of micronuclei (MN) by cytokinesis blocked micronucleus assay and morphology together with spectral fingerprints of nuclei using enhanced dark-field microscopy (EDFM) assembled with a hyperspectral imaging (HI) module. The combination of IONPDOX with ion carbon or photon irradiation increased the lethal effects of irradiation alone in correlation with the induction of MN. Alterations in the hyperspectral images and spectral profiles of nuclei reflected the CHS cell biological modifications following the treatments, highlighting possible new spectroscopic markers of cancer therapy effects. These outcomes showed that the proposed combined treatment is promising in improving CHS radiotherapy.
ERG K+ channels mediate a major component of action potential repolarization in lymphatic muscle
Authors: Kim Hae Jin , Li Min , Erlich Emma C. , Randolph Gwendalyn J. , Davis Michael J.
Published: September 2023

Smooth muscle cells in the walls of collecting lymphatic vessels fire spontaneous action potentials (APs), which conduct rapidly over the muscle layer to initiate contractions that propel lymph. Several ion channels have been implicated in the currents underlying the AP spike and the preceding diastolic depolarization, but the molecular identities of K+ channels involved in AP repolarization are unknown. Based on previous studies of other rhythmically active smooth muscles, we hypothesized that ether-a-go-go related gene (ERG) K+ channels (Kv11) play an important role in repolarization of the AP in lymphatic muscle. Message for one or more ERG channel isoforms was detected by RT-PCR analysis of lymphatic vessels from mice, rats and humans. Membrane potential recordings in smooth muscle cells of rat and human lymphatics revealed that nanomolar concentrations of ERG-1 inhibitors (E-4031 and BeKm-1) prolonged the duration of the AP plateau (normally ~ 1 s in duration) and induced multiple spikes, whereas ERG-1 activators (ICA-105574 and RPR-260243) shortened the plateau and could completely inhibit spontaneous APs. At relatively high inhibitor concentrations, the AP plateau duration lasted as long as 24 s. ERG activators reversed the effects of ERG inhibitors and vice-versa. In pressure myograph studies, ERG channel inhibition prolonged the diastolic repolarization phase of the contraction cycle and reduced the frequency of spontaneous contractions. This is the first evidence for a specific K+ channel contributing to the AP in lymphatic muscle. Our results imply that lymphatic contractile dysfunction may occur in long QT type II patients with mutations that result in ERG channel loss-of-function or impaired trafficking of the channel to the cell membrane.
Effect of different garments on thermophysiological and psychological comfort properties of athletes in a wear trial test
Authors: Özkan Esra Taştan , Kaplangiray Binnaz , Şekir Ufuk , Şahin Şenay
Published: September 2023

This paper reports on an experimental investigation of thermophysiological and psychological responses during and after an incremental low- to high-intensity exercise at 27 °C and 45% humidity. Five t-shirt garments were produced from different yarn types, their weights and yarn counts were close to each other. During the wear trials, heat and humidity sensors were placed at four body locations (the chest, back, abdomen, and waist). In addition, dynamic comfort measurements of the upper body were examined using a datalogger and subjective rating scales. This study aimed to investigate the effects of garment type on aerobic performance, microclimate temperature and humidity values, and psychological comfort. It was observed that the relative humidity and temperature of the microclimate were low in fabrics with high air permeability and low thermal resistance values of the Tencel single jersey and polyester mesh knitted fabrics. There was a significant difference in microclimate temperature results of TS coded Tencel single jersey t-shirt sample and other t-shirt samples according to statistical analysis results. On the other hand, the statistical results of the PM coded fabric sample measured at lower humidity in the three body regions were found to be a significantly different from those of the other samples (except TS). Although not statistically significant, the VO2 values and heart rates of these fabrics were lower than those of other fabrics. It was concluded that garments made from Tencel single jersey (TS) and polyester mesh (PM) fabrics affected the performance of athletes positively. Athletes were less forced during the training, and the activity could be maintained more than the others when wearing these clothes.