bluuebunny/crossref_metadata_2025 huggingface dataset:
Konsep Kepemimpinan Kepala Sekolah
Authors: Julaiha Siti
Published: June 2020

Sekolah merupakan suatu lembaga yang mempunyai berbagai komponen pendidikan yang harus dikelola oleh seorang manajer sekaligus leader dalam lembaga pendidikan.  Kepala sekolah sebagai seorang pemimpin mempunyai peran aktif dan senantiasa berpengaruh dalam segala masalah yang berkaitan denngan kebutuhan staff, guru dan siswa di sekolah. Kepala sekolah bertanggung jawab terhadap keberhasilan penyelenggaraan pendidikan dengan cara melaksanakan administrasi sekolah denngan seluruh substansinya, disamping itu kepala sekolah bertanggung jawab terhadap kualitas sumber daya yang ada agar mereka mampu menjalankan tugas-tugas sesuai dengan tugas dan fingsi masing-masing. Begitu beratnya tugas seorang kepala sekolah yang ditugaskan untuk mengkoordinir seluruh kegiatan di sekolah ditambah dengan kewajiban mengajarnya, namun dengan adanya PP No 19 tahun 2017 kepala sekolah dapat bernapas lega karena mereka tidak diwajibkan mengajar lagi. Namun demikian,  kompetensi dan standar untuk menjadi kepala sekolah harus tetap diperhatikan dan menjadi syarat ketentuan yang berlaku untuk menjadi kepala sekolah.
Pengembangan Media Pembelajaran Tematik Berbasis Macromedia Flash untuk Meningkatkan Hasil Belajar Siswa Madrasah Ibtidaiyah
Authors: Syabrina Muhammad , Sulistyowati Sulistyowati
Published: June 2020

Penelitian pengembangan ini bertujuan untuk 1) mengembangkan media pembelajaran tematik berbasis Macromedia Flash untuk meningkatkan hasil belajar siswa Madrasah Ibtidaiyah, 2) mengetahui efektifitas dan kemenarikan media pembelajaran, 3) mengetahui peningkatan hasil belajar siswa setelah menggunakan media pembelajaran. Penelitian pengembangan  ini mengadopsi  model desain pengembangan ADDIE (Analysis, Design, Development, Implementation, and Evaluation). Pengembangan ini menghasilkan produk berupa media pembelajaran tematik Berbasis Macromedia Flash pada tema “Indahnya Kebersamaan” subtema “Keberagaman Budaya Bangsaku” di Madrasah Ibtidaiyah. Hasil penelitian menunjukkan bahwa media pembelajaran yang dihasilkan memiliki tingkat efektivitas dan kemenarikan yang tinggi. Hal ini ditunjukkan dengan hasil uji coba berada pada kategori baik menurut skala 5. Adapun hasil validasi dan uji coba yang telah dilakukan adalah sebagai berikut: (1) Validasi ahli materi 94,00%, (2) Validasi ahli media pembelajaran 87,00%, (3) Validasi guru wali kelas IV 92,00%, (4) Uji coba perorangan 78,78%, (5) Uji coba kelompok kecil 84,54%, (6) Uji coba lapangan 85,78%. Nilai hasil belajar siswa yang dilihat dari peningkatan hasil pre-test dan post-test dengan nilai rata-rata siswa sebesar 55,13 dan nilai rata-rata pada tahap post-tets sebesar 91,71. Nilai rata-rata siswa mengalami kenaikan sebesar 36,58.
Pengaruh Suasana Keagamaan dan Kegiatan Rohis Nurul Aulad Terhadap Perilaku Siswa SMA Negeri 2 Samarinda
Authors: Albania Putri Rosyidatul , Saleh Khairul , Razak Abdul
Published: June 2020

Suasana keagamaan memiliki peran dalam meningkatkan perilaku baik siswa. Begitu pula dengan kegiatan Rohis yang diadakan di sekolah sebagai bentuk pembiasaan pada diri sendiri. Adanya suasana keagamaan tanpa kegiatan Rohis, maka perilaku siswa tidak akan menjadi budaya yang dapat diterapkan dalam kehidupan sehari-hari. Begitu sebaliknya, adanya kegiatan Rohis tanpa suasana keagamaan, maka perilaku siswa hanya akan menjadi pembiasaan tanpa penghayatan dan transinternalisasi nilai-nilai agama atau moral dalam diri. Oleh karena itu, penelitian ini bertujuan untuk mengetahui ada atau tidak adanya pengaruh suasana keagamaan terhadap perilaku siswa, ada atau tidak adanya pengaruh kegiatan Rohis Nurul Aulad terhadap perilaku siswa, dan ada atau tidak adanya pengaruh suasana keagamaan dan kegiatan Rohis Nurul Aulad secara bersama-sama terhadap perilaku siswa di SMA Negeri 2 Samarinda. Adapun jenis penelitian ini adalah jenis penelitian kuantitatif dengan teknik pengumpulan data berupa angket, observasi, dan dokumentasi. Teknik analisis data yang digunakan oleh peneliti yaitu regresi linear ganda. Populasi dalam penelitian ini seluruh siswa yang beragama Islam di SMA Negeri 2 Samarinda yang berjumlah 970 siswa. Teknik pengambilan sampel yang digunakan oleh peneliti adalah teknik probability sampling dengan fokus pengambilan sampel pada teknik proportionate stratified random sampling menggunakan rumus Slovin sehingga diperoleh sampel sebanyak 91 siswa. Hasil penelitian menunjukkan bahwa nilai  yaitu 0,623 dengan intensitas kuat dan nilai  = 27,895  = 3,10 sehingga Ha yang menyatakan bahwa adanya pengaruh suasana keagamaan dan kegiatan Rohis Nurul Aulad secara bersama-sama terhadap perilaku siswa dapat diterima.
Strategi Berbasis Motivasi dalam Pengajaran Tahfidzul Quran si SMP IT Imam Syafi'i Samarinda
Authors: Camma Alimuddin , Arnani Arnani , Ramdana Ramdana , Pambudi Suratman
Published: June 2020

Program menghafal al-qur’an SMP IT Imam Syafi’i Samarinda yang sudah empat tahun berjalan membawa semangat tersendiri bagi sekolah dalam upaya mencetak generasi yang qur’ani. Salah satu usaha yang dilakukan oleh yayasan, sekolah dan pembimbing ialah dengan melakukan strategi dalam pengajaran menghafal atau tahfidz qur’an. Memotivasi murid atau calon penghafal dalam upaya untuk menghafal al-qur’an adalah merupakan satu dari banyaknya strategi pembelajaran yang digunakan oleh pembimbing yang melibatkan motivasi dari yayasan, sekolah dan tentunya orang tua peserta didik. Penelitian ini merupakan penelitian lapangan (field work research) yang bertujuan untuk mengetahui strategi berbasis motivasi di SMP IT Imam Syafi’I Samarinda dalam memberikan semangat kepada peserta didik dalam menghafal al-qur’an. Sumber data dalam penelitian ini ialah kepala sekolah, waka kurikulum dan beberapa guru. Dalam pengumpulan data penulis menggunakan beberapa teknik yaitu observasi, wawancara dan dokumentasi. Dengan menggunakan analisis miles dan huberman dengan reduksi data, penyusunan data dan penarikan kesimpulan. Temuan penulis dalam penelitian ini yaitu : Pertama, motivasi yang diberikan kepada peserta didik meliputi motivasi material dan non- material, motivasi material yang diberikan ialah berupa penghargaan dari sekolah berupa beasiswa dan hadiah, adapun motivasi non- material berupa pujian, dorongan semangat dan muhasabah diri pada peserta didik. Kedua, adanya semangat peserta didik dalam menghafal al-qur’an yang didukung oleh yayasan, sekolah, guru serta orang tua menambah motivasi peserta didik dan mereka juga mampu mengikuti kegiatan pembelajaran baik itu formal dan non- formal dengan sangat baik.
Neonatal hand, foot, and mouth disease due to Coxsackievirus A6 in Shanghai
Authors: Xu Shanshan , Li Huajun , Qiao Peng , Xu Guofeng , Zhao Dongying , Lin Xiaoyan , Qin Yu , Yu Huiju , Zhang Xi , Zhang Wanju , Huang Lisu
Published: June 2020

Background: Evidence of hand, foot, and mouth disease (HFMD) in neonates is limited. The aim of this study was to evaluate the clinical symptoms, pathogens, possible transmission routes, and prognosis of neonatal HFMD in Shanghai. Methods: This was a case-control study based on the HFMD registry surveillance system. All neonates and infected family members were enrolled between 2016 and 2017 in Shanghai. Neonates with HFMD were followed for at least half a year. Detailed questionnaires, medical history, and physical examination were recorded. Routine blood examination, liver and renal function, immunophenotypes of peripheral blood lymphocytes (CD3, CD4, and CD8 T-cells; NK cells), immunoglobulin (Ig) M, IgG, and IgA, and cytokine interleukin (IL-1β, IL-2R, IL-6, IL-8, IL-10, and TNF-α) levels were measured. All rectal swab specimens were collected and genotyped for enterovirus, and phylogenetic analysis based on the VP1 sequences of coxsackievirus A6 (CV-A6) was performed to investigate molecular and evolutionary characteristics. T-test or nonparametric test was used to evaluate the differences. Logistic analysis was applied to calculate the risk of clinical manifestations in the group of HFMD neonates and their paired siblings. Results: There were 16 neonates among the 12608 diagnosed patients with HFMD, accounting for 0.13%. All neonatal infections were transmitted by other members of the family, mainly the elder siblings, and were caused by CV-A6. CV-A6 was the emerging and predominant causative agent of HFMD in Shanghai. None of the neonates with HFMD experienced fever, onychomadesis, or severe complications. However, two elder sibling patients showed lethargy, and one developed hypoperfusion. In the elder siblings with HFMD, the proportion of white blood cells was generally higher than in neonates with HFMD. The immunologic function of the neonates with HFMD was basically normal. The levels of inflammatory markers were higher in both neonates and elder siblings with HFMD compared to age-matched controls. The clinical symptoms receded about one week after onset. None of the neonates had sequelae. Conclusions: In our study, CV-A6 infection in neonates was benign, but had the character of family clustering. Due to the two-child policy in China, elder siblings may be the main route of HFMD transmission.
MicroRNA-200b reduced ZEB1 to inhibit cell proliferation and migration in human gastric cancer
Authors: Chen He , Liu Pengcheng , Wang Lei , Yu Yanxia , Zhao Yan , Cai Yihong , Wang Zhongxin , Zhang Min
Published: June 2020

BACKGROUND: Gastric cancer (GC) is one of the most common malignant cancers, with high morbidity and mortality rates worldwide. The present study was to explore whether miR-200b is a tumor suppressor in GC and to unveil the potential mechanisms. METHODS: Levels of c-Myc, Cyclin D1, MMP-3 and MMP-9 expression were detected respectively by qRT-PCR and Western blot assay. BrdU proliferation assay, Cell cycle analysis, Wound-healing and Transwell assays were used to study the role of miR-200b with inhibitor, mimics or ZEB1-RNAi in TGF-β1-treated SGC-7901/DDP cells. The xenograft model with nude mice was established to unveil the role of miR-200b in vivo.RESULTS: Compared with the paracancerous tissues, miR-200b was decreased in GC patients and SGC-7901/DDP cells. Lower level of miR-200b induced by its inhibitor promoted TGF-β1-treated SGC-7901/DDP cells proliferation and migration, and increased the levels of c-Myc, Cyclin D1, MMP-3, MMP-9, β-catenin and APC. Interestingly, miR-200b mimics and ZEB1-RNAi were able to reduce the proliferation and migration of TGF-β1-induced SGC-7901/DDP cells as well as their levels of c-Myc, Cyclin D1, MMP-3 MMP-9, β-catenin and APC. In addition, ZEB1 was indeed the potential target of miR-200b identified by dual luciferase reporter gene assay. Xenograft model also suggested that over-expression of miR-200b suppressed the growth of tumor in vivo. CONCLUSION: Taken together, our findings suggest that miR-200b be likely to play an important role in activating TGF-β1-induced SGC-7901/DDP cells and perform as a tumor suppressor by targeting ZEB1 in GC. What’s more, miR-200b may modulate Wnt/β-catenin signaling pathway in TGF-β1-induced SGC-7901/DDP cells.
Rotation and torsion of the left ventricle with cardiovascular magnetic resonance tagging: comparison of two analysis methods
Authors: Lehmonen Lauri , Jalanko Mikko , Tarkiainen Mika , Kaasalainen Touko , Kuusisto Johanna , Lauerma Kirsi , Savolainen Sauli
Published: June 2020

Background: Left ventricle rotation and torsion are fundamental components of myocardial function, and several software packages have been developed for analysis of these components. The purpose of this study was to compare the suitability of two software packages with different technical principles for analysis of rotation and torsion of the left ventricle during systole.Methods: A group of hypertrophic cardiomyopathy (HCM) patients (N = 14, age 43 ± 11 years), mutation carriers without hypertrophy (N = 10, age 34 ± 13 years), and healthy relatives (N = 12, age 43 ± 17 years) underwent a cardiovascular magnetic resonance examination, including spatial modulation of magnetization tagging sequences in basal and apical planes of the left ventricle. The tagging images were analyzed offline using a harmonic phase image analysis method with Gabor filtering and a non-rigid registration-based free-form deformation technique. Left-ventricle rotation and torsion scores were obtained from end-diastole to end-systole with both software. Results: Analysis was successful in all cases with both software applications. End-systolic torsion values between the study groups were not statistically different with either software. End-systolic apical rotation, end-systolic basal rotation, and end-systolic torsion were consistently higher when analyzed with non-rigid registration than with harmonic phase-based analysis (p < 0.0001). End-systolic rotation and torsion values had significant correlations between the two software (p < 0.0001), most significant in the apical plane.Conclusions: When comparing absolute values of rotation and torsion between different individuals, software-specific reference values are required. Harmonic phase flow with Gabor filtering and non-rigid registration-based methods can both be used reliably in the analysis of systolic rotation and torsion patterns of the left ventricle.
Prevalence and phylogeny of Chlamydiae and hemotropic mycoplasma species in captive and free-living bats
Authors: Fritschi Janine , Marti Hanna , Seth-Smith Helena M.B. , Aeby Sébastien , Greub Gilbert , Meli Marina L. , Hofmann-Lehmann Regina , Mühldorfer Kristin , Stokar-Regenscheit Nadine , Wiederkehr Danja , Pilo Paola , Broek Peggy Van Den , Borel Nicole
Published: June 2020

Background: Bats are hosts for a variety of microorganisms, however, little is known about the presence of Chlamydiales and hemotropic mycoplasmas. This study investigated 475 captive and free-living bats from Switzerland, Germany, and Costa Rica for Chlamydiales and hemotropic mycoplasmas by PCR to determine the prevalence and phylogeny of these organisms.Results: Screening for Chlamydiales resulted in a total prevalence of 31.4%. Positive samples originated from captive and free-living bats from all three countries. Sequencing of 15 samples allowed the detection of two phylogenetically distinct groups. These groups share sequence identities to Chlamydiaceae, and to Chlamydia-like organisms including Rhabdochlamydiaceae and unclassified Chlamydiales from environmental samples, respectively.PCR analysis for the presence of hemotropic mycoplasmas resulted in a total prevalence of 0.7%, comprising free-living bats from Germany and Costa Rica. Phylogenetic analysis revealed three sequences related to other unidentified mycoplasmas found in vampire bats and Chilean bats.Conclusions: Bats can harbor Chlamydiales and hemotropic mycoplasmas and the newly described sequences in this study indicate that the diversity of these bacteria in bats is much larger than previously thought. Both, Chlamydiales and hemotropic mycoplasmas are not restricted to certain bat species or countries and captive and free-living bats can be colonized. In conclusion, bats represent another potential host or vector for novel, previously unidentified, Chlamydiales and hemotropic mycoplasmas.
Comparison of the effect of rapeseed oil or amaranth seed oil supplementation on weight loss, body composition, and changes in the metabolic profile of obese patients following 3-week body mass reduction program: A randomized clinical trial
Authors: Moszak Małgorzata , Zawada Agnieszka , Juchacz Aldona , Grzymisławski Marian , Bogdański Paweł
Published: June 2020

Background: Amaranth seed oil (ASO) and rapeseed oil (RSO) are functional foods that display antioxidant and hepatoprotective properties. These oils are also known to lower glucose and cholesterol levels. The current study compared the effects exerted by RSO and ASO on weight loss and metabolic parameters during a 3-week body mass reduction program.Methods: Eighty-one obese subjects (BMI > 30 kg/m2), aged 25-70 years, were enrolled in a 3-week body mass reduction program based on a calorie-restricted diet and physical activity. Participants were randomly categorized into an AO group (administered 20 mL/d of ASO), a RO group (administered 20 mL/d of RSO), and a C group (control; untreated). Anthropometric and metabolic parameters were measured at baseline and endpoint. Results: Significant decreases in weight, body mass index (BMI), waist circumference (WC), hip circumference (HC), fat mass (FM), lean body mass (LBM), visceral fat mass (VFM), and total body water (TBW%) were observed in all groups (P < 0.05). No significant improvements were observed in the clinical parameters of group C. Fasting insulin (Δ -5.9, and Δ -5.7) and homeostatic model assessment of insulin resistance (HOMA-IR) (Δ -1.1 and Δ -0.5) were decreased in both RO and AO groups, respectively. Fasting glucose (Δ -8.5; P = 0.034), total cholesterol (Δ -14.6; P = 0.03), non-HDL cholesterol (Δ 15.9; P = 0.01), TG/HDL ratio (Δ -0.6; P = 0.032), LDL cholesterol (Δ -12.3; P = 0.042), and triglycerides (Δ -6.5; P = 0.000) were significantly improved in the AO group, compared to the RO group. Conclusions: The 3-week body mass reduction intervention caused a significant reduction in the weight, BMI, WC, HC, FM, and VFM of all groups. Except for HOMA-IR, there were no statistical differences between the clinical parameters of all groups. However, a trend toward improved insulin levels and HDL% was noticeable in AO and RO. Therapies involving edible oils with high nutritional value, such as RSO and ASO, show potential for improving metabolic measurements during body mass reduction programs. Thus, obese patients undertaking weight reduction programs may benefit from RSO and ASO supplementation.Trial registration: retrospectively registered, DRKS00017708
Health information systems in developing countries: Case of African countries
Authors: KOUMAMBA Aimé Patrice , BISVIGOU Ulrick Jolhy , NGOUNGOU Edgard Brice , DIALLO Gayo
Published: June 2020

Background: In developing countries, health information systems (HIS) are experiencing more and more difficulties to produce quality data. The lack of reliable health related information makes it difficult to develop effective health policies. In order to understand the organization of HIS in African countries, we undertook a literature review.Methods: Our study was conducted using the PubMed and Scopus bibliographic search engines. Our inclusion criteria were: (i) all articles published between 2005 and 2019, (ii) including in their titles the keywords "health", "information", "systems", "system", "africa", "developing countries", "santé", "pays en développement", "Afrique", (iii) articles that are written in English or French, (iv) which deals with organizational and technical issues about HIS in African countries.Results: Fourteen retrieved articles out of 2492 were included in the study, of which 13 (92.9%) were qualitative. All of them dealt with issues related to HIS in 12 African countries. All 12 countries (100.0%) had opted for a data warehouse approach to improve their HIS. This approach, supported by the DHIS2 application, has enabled providing reliable data. However, 11 of the 12 countries (92.0%) were aligned with donors’ strategies and lacked any national strategy. Conclusion: This study suggests that the lack of a national health information management strategy will always be a threat to HIS performance in African countries. Ideally, rigorous upstream thinking to strengthen HIS governance should be undertaken by defining and proposing a coherent conceptual framework to analyze and guide the development and integration of digital applications into HIS over the long term.
Blood Neutrophil Counts are Associated with Exacerbation Frequency and Mortality in COPD
Authors: Lonergan Mike , Dicker Alison J , Crichton Megan L , Keir Holly R , Dyke Melissa K. Van , Mullerova Hana , Miller Bruce E , Tal-Singer Ruth , Chalmers James
Published: June 2020

Background: Identifying patients with COPD at increased risk of poor outcomes is challenging due to disease heterogeneity. Potential biomarkers need to be readily available in real-life clinical practice. Blood eosinophil counts are widely studied but few studies have examined the prognostic value of blood neutrophil counts (BNC).Methods: In a large population-based COPD registry in the East of Scotland (TARDIS: Tayside Allergic and Respiratory Disease Information System), BNC were compared to measures of disease severity and mortality for up to 15 years follow-up. Potential mechanisms of disease modification by BNC were explored in a nested microbiome substudy. Results: 178120 neutrophil counts were obtained from 7220 people (mean follow up 9 years) during stable disease periods. Median BNC was 5200cells/µL (IQR 4000-7000cells/µL). Mortality rates among the 34% of patients with elevated BNCs (defined as 6000-15000cells/µL) at the study start were 80% higher (14.0/100 person years v 7.8/100py, P<0.001) than those with BNC in the normal range (2000-6000cells/µL). People with elevated BNC were more likely to be classified as GOLD D (46% v 33% P<0.001), have more exacerbations (mean 2.3 v 1.3/year, P<0.001), and were more likely to have severe exacerbations (13% vs. 5%, P<0.001) in the following year. Eosinophil counts were much less predictive of these outcomes. In a sub-cohort (N=276), patients with elevated BNC had increased relative abundance of Proteobacteria and reduced microbiome diversity.Conclusion: High BNC may provide a useful indicator of risk of exacerbations and mortality in COPD patients.
Healthy aging through the lens of community-based practitioners: a focus group study
Authors: Dev Rubee , Zaslavsky Oleg , Cochrane Barbara , Eagen Thomas , Woods Nancy F
Published: June 2020

Background: Nearly one in every seven Americans is 65 years and older, facing day-to-day challenge of aging. Although interest in healthy aging is growing, most of the efforts are directed towards understanding the perceptions of older adults. Little is known about the perspectives of community-based practitioners who work with older adults and deliver programs to promote healthy aging. The purpose of this project was to expand knowledge on healthy aging by exploring the perspectives of community-based practitioners working directly with older adults. Methods: We purposively sampled community-based practitioners (n=12, including nurses, physician, social workers, and other community services professionals) working with older adults, who then participated in one of three in-depth focus group discussions conducted between March and June 2016. Each focus group discussion lasted for about two hours. Verbatim transcript data were analyzed in Atlas.ti 7 using a conventional content analysis with an inductive approach, and consensual validation of coding was achieved.Results: Three core categories of healthy aging were identified: (1) characteristics of healthy aging; (2) healthy aging attainment; and (3) programs and activities for healthy aging. Practitioners identified a number of characteristics of healthy aging under person-specific (physiological, basic, psych-emotional, and cognitive needs), social aspects (creating community and contributing to the community), and spiritual dimensions (cultural views and beliefs) of healthy aging. Healthy aging attainment was represented as facilitators and barriers both with respect to care recipients and care providers, and programs and activities through promoting fitness and wellness. Conclusions: The rapidly changing demographics and aging population in the United States and their various needs suggest the implications for recognizing opportunities and developing and implementing programs to promote healthy aging. Although practitioners’ perspectives had some overlap with traditional research and medical views on healthy aging, the unique and holistic conceptual framework derived in the study might provide a more refined foundation for delivering appropriate health care services to the American aging population.
Educational Initiatives and Implementation of Electroencephalography into the Acute Care Environment: a protocol of a Systematic Review
Authors: Taran Shaurya , Ahmed Wael , Bui Esther , Prisco Lara , Hahn Cecil , McCredie Victoria A.
Published: June 2020

Background: Use of electroencephalography (EEG) is currently recommended by the American Clinical Neurophysiology Society for a wide range of indications, including diagnosis of nonconvulsive status epilepticus and evaluation of unexplained disorders of consciousness. Data interpretation usually occurs by expert personnel (e.g. epileptologists, neurophysiologists), with information relayed to the primary care team. However, data cannot always be read in time-sensitive fashion, leading to potential delays in EEG interpretation and patient management. Multiple training programs have recently been described to enable non-experts to rapidly interpret EEG at the bedside. A comprehensive review of these training programs, including the tools used, outcomes obtained, and potential pitfalls, is currently lacking. Therefore, the optimum training program and implementation strategy remain unknown. Methods: We will conduct a systematic review of descriptive studies, case series, cohort studies and randomized controlled trials assessing training programs for EEG interpretation by non-experts. Our primary objective is to comprehensively review educational programs in this domain and report their structure, patterns of implementation, limitations, and trainee feedback. Our secondary objective will be to compare the performance of non-experts for EEG interpretation with a gold standard (e.g. interpretation by a certified electroencephalographers). Studies will be limited to those performed in acute care settings in both adult and paediatric populations (intensive care unit, emergency department, or post-anesthesia care units). Comprehensive search strategies will be developed for MEDLINE, EMBASE, WoS, CINAHL, and CENTRAL to identify studies for review. The gray literature will be scanned for further eligible studies. Two reviewers will independently screen the search results to identify studies for inclusion. A standardized data extraction form will be used to collect important data from each study. If possible, we will attempt to meta-analyse the quantitative data. If heterogeneity between studies is too high, we will present meaningful quantitative comparisons of secondary outcomes as per the synthesis without meta-analysis (SWiM) reporting guidelines. Discussion: We will aim to summarize the current literature in this domain to understand the structure, patterns, and pitfalls of EEG training programs for non-experts. This review is undertaken with a view to inform future education designs, potentially enabling rapid detection of EEG abnormalities and timely intervention by the treating physician. PROSPERO registration: Submitted and undergoing review (URL currently unavailable).
Health-related quality of life of people living with HIV/AIDS: the role of social inequalities and disease-related factors
Authors: Nigusso Fikadu Tadesse , Mavhandu-Mudzusi Azwihangwisi Helen
Published: June 2020

Background: Health-related quality of life (HRQoL) serves as a direct measure of individuals’ health, life expectancy and the impact that the utilization of health care has on quality of life. The purpose of this study is to assess the HRQoL of people living with HIV/AIDS (PLWHA), and to ascertain its association with the social inequalities and clinical determinants among people living with HIV in Benishangul Gumuz Regional State, Ethiopia.Methods: A cross-sectional study was conducted between December 2016 and February 2017; 390 people at two referral hospitals and three health centers participated in the study. The Patient-Reported Outcomes Measurement Information System Global Health Scale (PROMIS Global 10) was used to measure key HRQoL domains. Physical Health Summary (PHS) and Mental Health Summary (MHS) scores were employed. PHS and MHS scores below 50 (the standardized mean score) were determined to be poor and above 50 to be good. Bivariate and multivariate logistic regression analyses were used to identify factors associated with PHS and MHS scores. Results: This study included 259 (66.4%) females and 131 (33.6%) males. The PHS scores ranged from 16.2 to 67.7 with a mean of 48.8 (SD = 8.9). Almost 44.6% of the study population has a PHS score of below 50; the MHS scores ranged from 28.4 to 67.6 with a mean of 50.8 (SD = 8.1). About 41.8% of the study population has an MHS score of below 50. Unemployment, household food insecurity and comorbidities with HIV were associated with both poor PHS and poor MHS scores. Age below 25 years and being a member of Christian fellowship were inversely associated with poor PHS. The least wealth index score and CD4 count below 350 cells/mL were also associated with poor MHS. Conclusion: Overall, socioeconomic inequalities and HIV-related clinical factors play an important role in improving the HRQoL of PLWHA. Many of these determinants are alterable risk factors. Appropriate strategies can improve the holistic management of chronic HIV care and maximize PLWHAs’ HRQoL. Such strategies require the adoption of comprehensive interventions, including policies and programmes that would improve the health, wellbeing and livelihood of PLWHA.
Associations of participation in organized sports and physical activity in preschool children: a cross-sectional study
Authors: Chen Chu , Sellberg Fanny , Ahlqvist Viktor H. , Neovius Martin , Christiansen Filip , Berglind Daniel
Published: June 2020

Background Participation in organized sports is associated with higher physical activity (PA) levels in school-aged-children. Yet, little is known about PA determinants in preschool-aged-children. We examined associations between organized sports participation and preschoolers’ daily PA. Methods The study comprised 290 3-5 years old children and PA was measured for 1 week via accelerometers. Organized sports participation was parent-reported and preschool arrival and departure time was teacher-recorded. The preschool duration reported by teachers was matched with time-stamped accelerometer data to distinguish PA during preschool time and PA outside preschool time. Linear mixed models, nested on preschool level, were used to examine associations between organized sports participation and children’s PA outside preschool time, during preschool time and throughout the day. Results In total, 146 children (50.3%) participated in organized sports at least 1 hour/week. Participation in organized sports was associated with 6.0 more minutes of moderate-to-vigorous PA (MVPA) (95% CI: 0.6, 11.3) throughout the day and 5.7 more minutes of MVPA (95% CI: 1.6, 9.7) outside preschool time after adjustment. There was no association between organized sports participation and PA during preschool time. Conclusions This is the first study to show positive associations between organized sports participation and preschoolers’ PA levels outside preschool time and throughout the day. In addition, findings from this study do not support PA compensation. Therefore, targeting organized sports may be successful in improving PA, even among preschoolers.
Deep Sequencing Reveals New Roles for MuB in Transposition Immunity and Target-capture, and Redefines the Insular Ter Region of E. coli
Authors: Walker David M , Harshey Rasika
Published: June 2020

Background The target capture protein MuB is responsible for the high efficiency of phage Mu transposition within the E. coli genome. However, some targets are off-limits, such as regions immediately outside the Mu ends (cis-immunity) as well as the entire ~37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is responsible for cis-immunity and is also implicated in Mu genome immunity, but via different mechanisms. This study was undertaken to dissect the role of MuB in target choice in vivo.Results We tracked Mu transposition from six different starting locations on the E. coli genome, in the presence and absence of MuB. The data reveal that Mu’s ability to sample the entire genome during a single hop in a clonal population is independent of MuB, and that MuB is responsible for cis-immunity, plays a minor role in Mu genome immunity, and facilitates insertions into transcriptionally active regions. Unexpectedly, transposition patterns in the absence of MuB have helped extend the boundaries of the insular Ter segment of the E. coli genome.Conclusions The results in this study demonstrate unambiguously the operation of two distinct mechanisms of Mu target immunity, only one of which is wholly dependent on MuB. The study also reveals several interesting and hitherto unknown aspects of Mu target choice in vivo, particularly the role of MuB in facilitating the capture of promoter and translation start site targets, likely by displacing macromolecular complexes engaged in gene expression. So also, MuB facilitates transposition into the restricted Ter region of the genome.
Abnormal higher order aberrations in anisometropic amblyopia
Authors: Zhang Lingyi , Liao Meng , Zhang Wenqiu , Liu Longqian
Published: June 2020

Background

The study compared ocular higher-order aberrations (HOAs) between monocular anisometropic amblyopia children and similar anisometropia children with normal best-corrected visual acuity (BCVA). The amblyopia eyes could not reach the standard BCVA level of their age even after several months' regular treatment of amblyopia. We tried to find if these intractable amblyopia eyes have abnormal HOAs.
Methods

Fifty school-aged children (5–9 years) with hyperopic anisometropia were recruited at West China hospital clinic. Each subject shall be reexamined once every three months, four consecutive reexaminations for 12 months, of which only 25 subjects with normal BCVA after wearing glasses shall be included in the control group. The rest 25 subjects were treated by glasses and six months’ patching. Their interocular difference of visual acuity was still more than or equal to LogMAR 0.2, and the eyes with poor visual acuity did not reach the normal level in the twelfth month. These subjects were included in the amblyopia group. The BCVA, HOAs (5 mm pupil diameter), and axial length were recorded for all subjects.
Results

There were significant differences of higher order aberrations in C (3, -3) between the amblyopia eyes and the other three groups of eyes with normal BCVA (all p < 0.05). There were significant differences of higher-order aberrations in third-order root-mean-square aberrations(RMS) between the amblyopia eyes and the low-diopter eyes from two subject groups(both p < 0.05). Compared to high-diopter eyes in the control group, there were a significantly higher C(3,-3) in all amblyopia eyes and a significantly higher third-order RMS (p = 0.040) in the moderate-to-severe amblyopia group. In the Pearson correlation test, the C(3,-3) and third order RMS demonstrated statistically significant correlations with BCVA ( (r=-0.19, p = 0.04; r = 0.37, p < 0.01).
Conclusions

The anisometropic amblyopia eyes had different HOAs from the eyes with normal visual acuity. The third-order aberrations were the primary abnormal higher-order aberration in amblyopia eyes.
Development of Molecular Rapid Detection for Vibrio cholerae and Escherichia coli
Authors: Waturangi Diana Elizabeth , PETRUS JASON , KOSASIH RICO , RAISSA GLORIA
Published: June 2020

Background: Vibrio cholerae and Escherichia coli were main causative agent foodborne diseases, especially in many developing countries, such as Indonesia. Thereby, rapid detection of these pathogenic bacteria is necessary to quickly detect infection that occurred so it can be treated immediately. In this case, multiplex PCR allows multiple genes amplification in one reaction thereby enable to perform rapid detection of these pathogenic bacteria. The objective of this study is to develop rapid molecular detection of V. Cholerae and E. coli and analyze the sensitivity and specificity of this assay.Result: In this study, we used various virulence genes in each pathogenic bacteria as marker to develop rapid molecular detection. Based on this research, optimum results of V. cholerae and E. coli rapid detection were obtained with a primer concentration of 16 µM for ctxA and ompU, 30 µM for ace, and 50 µM for zot, and toxR; 2 µM for elt and 5 µM for stx, respectively. Finally, based on the method standardization by ISO/TS 20836 these assays had 0% false positive, 0% false negative, 100% specificity, and 100% sensitivity; 0% false positive, 4% false negative, 100% specificity, and 96% sensitivity for V. cholerae and E. coli respectively. Conclusion: The optimized method was qualified to be used as a detection method for V. cholerae and E. coli detection according to ISO/TS 20836 (2017) and EHEC and ETEC contamination in drinking water samples.
Metformin inhibits cervical cancer cell proliferation by modulating PI3K/Akt-induced major histocompatibility complex class I-related chain A gene expression
Authors: Xia Chenglai , Liu Chang , He Zhihong , Cai Yantao , Chen Jinman
Published: June 2020

Background: Recent studies have shown that the classic hypoglycemic drug metformin inhibits tumor growth; however, the underlying mechanism remains unclear. We previously showed that metformin disrupts the sponge effect of long non-coding RNA MALAT1/miR-142-3p to inhibit cervical cancer cell proliferation. In this study, we interrogated the ability of metformin to modulate the anti-tumor immune response in cervical cancer. Methods:The cell counting kit-8 assay was used to detect the viability of cervical cancer cells. Flow cytometry assays were performed to measure cell apoptosis and cell cycle. Lactate dehydrogenase (LDH) cytotoxicity assay was used to detect NK Cell Cytotoxicity. Relative protein levels were determined by immunoblotting and relative gene levels were determined by quantitative real-time PCR. Tumor Xenograft Modeling was used to evaluate the effect of metformin in vivo.Results: Metformin inhibited cervical cancer cell proliferation, cervical cancer xenograft growth, expression of PCNA, p-PI3K and p-Akt. Moreover metformin induced cervical cancer cell apoptosis and caused cancer cell cycle arrest. In addition, metformin upregulated the expression of DDR-1 and p53 in human cervical cancer cells. Furthermore, metformin also regulated the mRNA and protein expression of MICA and HSP70 on the surface of human cervical cancer cells via the PI3K/Akt pathway, enhancing NK cell cytotoxicity. Conclusions: In conclusion, our results suggest that metformin may be used as immunopotentiator to inhibit cervical cancer progression and may be considered a viable candidate for combination therapy with immunotherapy.
Job preferences for medical and nursing students to work in rural Guizhou Province, China: a discrete choice experiment
Authors: Bao Meiling , Huang Cunrui
Published: June 2020

Background Maldistribution of health workers between urban and rural areas has been a critical difficulty in China. The shortage of health workers in disadvantaged areas reduces access to essential health services delivery, and adversely affects the population health. Policies on attracting health workers to locate in rural areas are needed to be explored. In order to identify the appropriate incentives, we conducted a discrete choice experiment to determine how specific job attributes might be valued by final year students in medical university in Guizhou Province, China. Methods Attributes of potential job were developed through literature review, in-depth semi-structured interviews and pretest. Salary, education opportunity, transportation, job location, workload, essential equipment, medical order, and identification ('bianzhi') were included. The questionnaire was formulated through a fractional factorial experiment design using %MktRuns macros of SAS 9.4. All medical and nursing students in the final year at Guizhou Medical University were invited to participate in the study. Mixed logit model was used to estimate stated preferences of attributes. Willingness to pay and uptake rates for a defined job were also calculated based on the mixed logit estimates. Results The final sample comprised 787 respondents, including 388 medical students and 399 nursing students. Attributes were statistically significant (with the exception of once every two years for education opportunity) and had expected signs. The results indicate that physical conflict between doctors and patients and identification ('bianzhi') were two of the most important non-monetary job characteristics for both medical and nursing students. And nursing students placed more value on identification ('bianzhi'). Policy simulation suggests that as for the individual incentive respondents were most sensitive to salary increasing. Incentive packages effects were stronger for students from rural background. Conclusions Strategies on medical order, identification ('bianzhi') and salary should be considered to attract final year medical and nursing students to work in rural areas. In addition, specific recruitment policy design tailored for subgroups should be taken into account.
Analyzing the Impact of a State Concussion Law using an Autoregressive Integrated Moving Average Intervention Analysis
Authors: Huang Lihong , Sullivan Lindsay , Yang Jingzhen (Ginger)
Published: June 2020

Background: Existing studies analyzing the impact of state concussion laws have found an increase in concussion-related healthcare utilization post-law, in some instances, such increases were observed during the pre-law period due to a potential “spillover” effect. However, few studies have accounted for such an effect when assessing the impact of concussion laws. This study aimed to assess the effects of Ohio’s concussion law on monthly rates of concussion-related medical encounters in Medicaid insured children, using autoregressive integrated moving average (ARIMA) analysis. Methods: We analyzed claim data obtained from the Partners For Kids database, a pediatric accountable care organization in Ohio. Concussion-related medical encounters for Medicaid-insured children (ages 0-18 years) treated between April 1, 2008 to December 31, 2016 were selected and analyzed. The pre- and post-law trends in concussion-related medical encounters were assessed using an ARIMA intervention model. Results: A total of 16,943 concussion-related medical encounters sustained by 15,545 unique patients were identified. Three upward breaks in the monthly rates of concussion-related medical encounters were observed during the study period, with two breaks observed during the pre-law period. A seasonal trend was observed with the highest rates in September and October of each year. Conclusions: Using an ARIMA intervention model, we identified three upward breaks in the monthly rate of concussion-related medical encounters between 2009 and 2016 and an associated seasonal trend. Further research is needed to investigate the effect of state concussion laws on concussion-related medical encounters in children with other types of medical insurance.
Genome-wide Characterization, Evolution and Expression Profiling of Udp-glycosyltransferase Family in Pomelo (Citrus Grandis) Fruit
Authors: Wu Boping , Liu Xiaohong , xu kai , Zhang Bo
Published: June 2020

Background: Pomelo is one of the three major species of citrus. The fruit accumulates a variety of abundant secondary metabolites that affect the flavor. UDP-glycosyltransferases (UGTs) are involved in the glycosylation of secondary metabolites.Results: In the present study, we performed a genome-wide analysis of pomelo UGT family, a total of 145 UGTs was identified based on the conserved plant secondary product glycosyltransferase (PSPG) motif. These UGT genes were clustered into 16 major groups through phylogenetic analysis of these genes with other plant UGTs (A-P). Pomelo UGTs were distributed unevenly among the chromosomes. At least 10 intron insertion events were observed in these UGT genome sequences, and I-5 was identified to be the highest conserved one. The expression profile analysis of pomelo UGT genes in different fruit tissues during development and ripening was carried out by RNA-seq.Conclusions: We identified 145 UGTs in pomelo fruit through transcriptome data and citrus genome database. Our research provides available information on UGTs studies in pomelo, and provides an important research foundation for screening and identification of functional UGT genes.
Risk Perception and Behavioral Responses Related to COVID-19 Among the Iranian General Population: An Application of the Extended Parallel Process Model
Authors: Jahangiry Leila , Bakhtari Fatemeh , Sohrabi Zahara , Reihani Parvin , Samei Sirous , Ponnet Koen , Montazeri Ali
Published: June 2020

Background: The novel coronavirus disease 2019 (COVID-19) has emerged as a major global public health challenge. Psychosocial and cultural factors affect adherence to health advice. This study aims to investigate how people have perceived the COVID-19 outbreak using the components of EPPM (i.e., recommended response efficacy, self-efficacy, susceptibility, and severity) and how their behavioral responses contributed to the prevention and control of the disease.Methods: This cross-sectional study was conducted online in Iran in March and April. Data were collected using an electronic questionnaire via Porsline. Participants were recruited using online applications and posts on platforms such as Telegram, WhatsApp, and Instagram asking people to take part. The posts asked people aged 15 years and over to take part in a study investigating the control and prevention of COVID-19. EPPM was used to develop a questionnaire measuring the risk perception of and behavioral responses to COVID-19. We used a 29-item, standardized, structured format. Analysis of variance (ANOVA) and t-tests were used to compare groups. In all tests, a value of p < 0.05 was considered statistically significant.Results: A total of 3,727 individuals with a mean age (SD) of 37.0 (11.1) years participated in the study. The results by age category revealed significant progressive increases in perceived susceptibility, perceived self-efficacy, and avoidance response scores, particularly among those aged 60 and over. A total of 56.4% of participants were engaging in danger control processes and 43.6% in fear control processes. Women had significantly higher scores than men for self-efficacy, reactance, and avoidance defensive responses; men had higher perceived susceptibility scores for COVID-19 than women. We also found significantly higher scores for behavioral responses among people with advanced educational backgrounds. Conclusion: more than half of all participants motivated by danger control. This indicates that more than half of participants had high perceived efficacy (i.e., self-efficacy and response efficacy) scores. Self-efficacy scores were significantly higher among participants who were older, female, single, lived in rural areas, or had good economic status. The results of our study suggest that socioeconomic and demographic factors are the main determinants of psychological responses to controlling the COVID-19 pandemic.