bluuebunny/crossref_metadata_2025 huggingface dataset:
216 Effect of Spray Dried Plasma on the Standardized Ileal Digestibility of Crude Protein and Amino Acids in Diets Based on Different Ingredient Combinations Fed to Young Pigs
Authors: Bailey Hannah M , Stein Hans H , Campbell Joy
Published: May 2021

An experiment was conducted to test the hypothesis that spray dried plasma (SDP) increases the standardized ileal digestibility (SID) of CP and AA from other ingredients in diets for young pigs, which may result in a lack of additivity in such diets. Thirty ileal cannulated barrows (body weight: 9.30 ± 0.63 kg) were randomly allotted to a triplicated 10 × 3 Youden square design with 10 diets and three 7-d periods. Diets from four regions were formulated: the U.S.A. [corn-soybean meal (SBM)-based diet], the European Union (corn-wheat-barley-SBM-based diet), Canada (wheat-barley-SBM-fermented SBM-based diet), and Asia (corn-rice-SBM-fermented SBM-based diet). Diets from each region contained 0 or 6% SDP. Differences between measured and predicted SID of CP and AA in diets with SDP were calculated. Data were analyzed using PROC MIXED of SAS as a 2 × 4 factorial with 2 levels of SDP and 4 regions. There was an SDP by region interaction for the measured SID of CP and all AA, except Lys (Table 1). When SDP was added to the U.S.A, the European Union, and Asia diets, the SID of CP and AA was not increased. The SID of CP and AA, except Lys, was greater (P < 0.05) in the Canada diet with SDP than without SDP. The measured SID of CP and AA was consistent with predicted values for the European Union and Asia diets (Table 2). The measured SID of Trp was less (P < 0.05) than predicted for the U.S.A. diet, and the measured SID of CP and all indispensable AA was greater (P < 0.05) than predicted in the Canada diet. In conclusion, addition of 6% SDP to wheat-barley-based diets may increase SID of CP and AA, and therefore, the SID of CP and AA in diets containing SDP may be greater than predicted.
212 Conditioning and Expansion Increases Nutritional Value of Soybean Expellers
Authors: Espinosa Charmaine D , Oliveira Maryane S Sespere Faria , Limbach Joseph , Fanelli Natalia , Wiltafsky-Martin Markus , Stein Hans H
Published: May 2021

Two experiments were conducted to test the hypothesis that different combinations of conditioning and expansion of soybean expellers increases nutritional value. Non-heat-treated soybean expellers (L-1) and soybean expellers conditioned for 60 s at 90ºC followed by expansion at 110ºC (L-2) were used. Two additional sources of soybean expellers (L-3 and L-4) were processed as L-2 with the exception that the initial conditioning was followed by long-term conditioning for 12 or 48 min at 100ºC before expansion. Analyzed trypsin inhibitor activity in L-1, L-2, L-3, and L-4 was 34.0, 23.1, 4.2, and 2.4 mg/g, respectively. In experiment 1, 10 cannulated barrows (54.22 ± 4.54 kg) were allotted to a replicated 5 × 4 Youden square with 5 diets and 4 periods and 8 replicates per diet. Each source of soybean expellers was included in one diet, and a N-free diet was also used. Data were analyzed by ANOVA using the Mixed Procedure of SAS. The standardized ileal digestibility (SID) of all amino acids (AA) in L-1 was less (P < 0.01) compared with L-2, L-3, and L-4 (Table 1), and SID of all AA in L-2 was less (P < 0.01) than in L-3 or L-4. In experiment 2, 40 barrows (17.52 ± 1.63 kg) were housed in metabolism crates and fed a corn diet or 4 diets based on corn and each source of soybean expellers. Feces and urine were collected using the marker-to-marker approach with 5-d adaptation and 4-d collection periods. Data were analyzed as in Exp. 1. The metabolizable energy (ME) in L-1 was less (P < 0.01) than in L-2, L-3, and L-4 (Table 1). In conclusion, the SID of AA in soybean expellers was maximized if 12 or 48 min of conditioning at 100ºC was used before expansion, but long-term conditioning did not increase ME.
213 Increased Standardized Ileal Digestible Isoleucine to Lysine Ratio Improved Feed Efficiency in Pigs Fed Distillers Dried Grains with Solubles from 11 to 80 kg
Authors: Xue Pengcheng , Pilcher Chad M , Li Qingyun , Saddoris-Clemons Kari L
Published: May 2021

An experiment was conducted to determine the impact of dietary SID Ile to Lys ratio on the performance pigs from 11 to 80 kg of BW. A total of 1,092 pigs (initial BW = 11.1, SEM = 0.6 kg; 14 pens/treatment; 13 pigs/pen) were used in an 80d trial with a randomized complete block design. Dietary treatments were in a 2 x 3 factorial arrangement containing 2 levels of DDGS (0 and 25%) and 3 levels of SID Ile/Lys (0.54, 0.58, and 0.62). Energy and nutrient levels of all treatments were formulated at equal levels that met or exceeded NRC (2012) requirements. The SID Ile/Lys ratio was controlled by crystalline Ile. The MIXED procedures of SAS 9.4 were employed for statistical analysis. Orthogonal contrasts were used to test for main effects of DDGS and SID Ile/Lys. In the results, formulating with 25% DDGS decreased FBW, ADG, and ADFI (P < 0.05) of growing pigs. The average FBW of treatments without and with 25% DDGS were 81.7 and 78.6 kg, respectively. The ADG of diets without and with DDGS were 0.88 vs 0.84 kg/d, respectively. The ADFI of treatments without DDGS were 1.85 kg/day, while the counterparts of treatments fed diets containing 25% DDGS were 1.79 kg/d. Feeding diets containing 25% DDGS tended to decrease Gain:Feed (0.477 vs. 0.472; P < 0.10). Increasing the level of SID Ile/Lys (from 0.54 to 0.62) linearly increased Gain:Feed in pigs fed diets containing 25% DDGS (0.467, 0.471, 0.477, respectively; P < 0.05). In summary, this study demonstrated feeding diets containing 25% DDGS decreased the performance of growing pigs by reducing ADG and ADFI. Greater SID Ile/Lys in diets may help reduce the negative impact of DDGS diets in grow-finishing pigs by improving feed efficiency.
217 Effects of Dietary Protein Content and Crystalline Amino Acid Supplementation Patterns on Growth Performance of Weaned Pigs Raised Under Different Sanitary Conditions
Authors: Lee Jinyoung , González-Vega J Caroline , Htoo John K , Yang Chengbo , Nyachoti Martin
Published: May 2021

The objective of this experiment was to determine the effects of dietary crude protein (CP) content and crystalline amino acids (CAA) supplementation patterns on the growth performance of weaned pigs under clean (CSC) or unclean sanitary conditions (USC). One hundred forty-four piglets (6.35 ± 0.63 kg BW) were housed under CSC or USC for 3 wk and assigned to 1 of 3 diets: a high CP (HCP; 21%) and two low CP (LCP; 18%) diets supplemented with 10 crystalline indispensable amino acids (IAA) to meet all IAA requirements or only 6 IAA (Lys, Met, Thr, Trp, Val, and Ile) to meet IAA requirements except Leu, His, and Phe. Each treatment had 8 replicates (3 pigs per pen) per sanitary condition. The CSC room was cleaned and washed weekly. For the USC room, sow manure was spread and remained unwashed. Data were analyzed using the MIXED procedure of SAS with orthogonal polynomial contrasts. Pigs raised under USC had reduced (P < 0.05) ADG and G:F in wk 2, but overall, no difference was found between sanitary conditions due to contrary results in wk 3. Overall, ADG and ADFI were not affected but G:F tended (P < 0.10) to be lower for USC pigs. Also, G:F did not differ between HCP (0.79) and LCP (0.81) under CSC, however, LCP interactively decreased (P < 0.05) G:F to 0.75 compared to 0.83 in HCP under USC. The CAA supplementation patterns did not influence growth except reduced (P < 0.05) ADFI in wk 3. Pigs fed the HCP diet had higher (P < 0.05) fecal scores throughout the experiment than those fed LCP diets under both sanitary conditions. In conclusion, overall growth performance did not differ between HCP and LCP under CSC, but LCP diets reduced G:F under USC. The fecal score decreased in LCP diets regardless of sanitary conditions.
210 A Longer Adaptation Period to a Functional Amino Acid-supplemented Diet Improves Growth Performance and Attenuates Acute-phase Response in Salmonella Typhimurium-challenged Pigs
Authors: Rodrigues Lucas , Wellington Michael O , González-Vega J Caroline , Htoo John K , Van Kessel Andrew G , Columbus Daniel A
Published: May 2021

Functional amino acid supplementation during disease challenge enhances growth performance and immune status. The present study investigated the effect of duration of adaptation period to a functional amino acid (FAA)-supplemented diet on growth performance and immune status during a subsequent Salmonella challenge in pigs. Thirty-two mixed-sex weanling pigs (8 pigs/treatment; 11.6 ± 0.34 kg initial body weight) received either a basal diet without FAA supplementation throughout the experimental period (FAA-) or a diet containing a supplemented FAA profile (Thr, Met, and Trp at 120% of requirements) fed for either 0 (FAA+0), 1 (FAA+1) or 2 (FAA+2) wk pre- and 1 wk post-inoculation with Salmonella Typhimurium (ST). Pigs were orally inoculated with saline containing ST after the 2 wk pre-inoculation period and monitored for 1 wk post-inoculation. Pigs had ad libitum access to diets throughout the experiment. Performance parameters [average daily gain (ADG), feed intake, and gain:feed (GF)] were measured in the pre- and post-inoculation periods. Blood samples were collected on d 0, 4, and 7 post-inoculation for serum haptoglobin and albumin analysis. There was no effect of diet on pre-inoculation performance (P > 0.05). Post-inoculation, FAA+2 pigs had the highest ADG (0.46 kg/d) and GF (0.63 kg/kg), FAA- the lowest (0.21 kg/d; 0.29 kg/kg), with FAA+0 (0.33 kg/d; 0.46 kg/kg) and FAA+1 (0.40 kg/d; 0.53 kg/kg) being intermediate (ADG, P < 0.05, SEM=0.059; GF, P < 0.05, SEM=0.099). Overall, albumin was higher in FAA+2 (35.25 g/L) and FAA+1 (34.63 g/L) pigs compared to FAA+0 (30.38 g/L) and FAA- (29.67 g/L) pigs (P < 0.05, SEM=0.717). Furthermore, FAA+2 pigs had the lowest overall haptoglobin (0.90 g/L), FAA- the highest (1.54 g/L), with FAA+0 (1.32 g/L) and FAA+1 (1.06 g/L) being intermediate (P < 0.05, SEM=0.111). In conclusion, a longer adaptation period to FAA supplementation improved performance and attenuated the immune response of pigs when exposed to an enteric disease challenge.
211 A Corn Protein Product Has Greater Concentration of Digestible Amino Acids and Energy Than Low-oil Distillers Dried Grains with Solubles When Fed to Pigs and May Be Used in Diets for Weanling Pigs
Authors: Medellin Jessica P Acosta , Espinosa Charmaine D , Jaworski Neil , Stein Hans H
Published: May 2021

The hypothesis that the nutritional value of corn protein (approximately 50% protein) is greater than in two sources of distillers dried grains with solubles (DDGS1 and DDGS2) was tested. In Exp. 1, eight ileal-cannulated barrows (37.1 ± 2.4 kg) were allotted to a Latin square and fed a N-free diet or diets based on corn protein, DDGS1, or DDGS2. Data were analyzed by ANOVA using SAS with treatment and replicate being fixed and random effects, respectively. Concentrations of standardized ileal digestible (SID) AA were greater (P < 0.05) in corn protein than in DDGS (Table 1). In Exp. 2, 32 barrows (16.5 ± 0.9 kg) in metabolism crates received diets based on, corn, corn protein, DDGS1, or DDGS2. Data were analyzed as in Exp. 1. Corn protein had greater (P < 0.01) ME compared with DDGS1 and DDGS2 (Table 1). In Exp. 3, 160 pigs (6.02 ± 0.84 kg) were allotted to 4 treatments with 8 pens per treatment. Phase 1 and 2 diets contained 0, 5, 5, or 10% and 0, 2.5, 7.5, or 10% corn protein, respectively, which replaced enzyme-treated soybean meal (HP300) or plasma in phase 1 or HP300 in phase 2. Diets were formulated using ME and SID AA values from Exp. 1 and 2. A common phase 3 diet was used. Data were analyzed as in Exp. 1. At the end of phase 1, BW was reduced (P < 0.05) for pigs fed the diet with the greatest inclusion of corn protein, but there was no difference at the end of the experiment (Table 2). Fecal score tended (P = 0.066) to be reduced in phase 2 for pigs fed the greatest inclusion of corn protein. In conclusion, corn protein has greater ME and digestible AA than DDGS and may reduce fecal scores of pigs
184 Evaluation of Aviplus on Growth Performance of Nursery and Growing-finishing Pigs
Authors: Hutchens Wade M , Tokach Mike D , Woodworth Jason C , DeRouchey Joel M , Goodband Robert D , Calderon Hilda I , Keppy Kaylee , Stephens Kevin , Maynard Philip
Published: May 2021

A total of 1,215 pigs (L337×1050, PIC, Hendersonville, TN) were used in a 156-d wean-to-finish experiment. Pigs were weaned at 21 d of age and placed in pens based on initial body weight (BW) with 27 pigs/pen. There were three dietary treatments including a: 1) control; 2) pigs fed diets containing AviPlus (micro-encapsulated sorbic and citric acids and synthetic thymol and vanillin botanicals; Vetagro Inc., Chicago, IL) during the nursery and finisher phases; or 3) pigs fed AviPlus during the nursery but not the finishing phase. AviPlus was included at 2.72 kg/ton from d 0 to 21, 0.90 kg/ton from d 21 to 42, and 0.45 kg/ton from d 42 to 156. Thus, there were 15 control pens and 30 Aviplus pens in the nursery and 15 pens for the 3 treatments in the finisher phase. On d 42, pigs were transported as intact pens to the finishing facility. Data were analyzed as a RCBD with pen as experimental unit. For the overall nursery period (d 0 to 42), pigs fed AviPlus had improved (P < 0.05) G:F, with no evidence of difference (P > 0.05) for d 42 BW, ADG, or ADFI. For the overall finishing period (d 42 to 156) and overall experimental period (d 0 to 156), there was no evidence of difference (P > 0.05) for BW, ADG, ADFI, or G:F. There was no evidence of difference (P > 0.05) observed for mortality and removals during the nursery, finisher, or overall. In summary, providing AviPlus during the nursery improved nursery G:F, but there was no effect on overall wean-to-finish performance.
187 Effects of Oligosaccharide-based Polymer on Growth Performance, Diarrhea, and Fecal β-hemolytic Coliforms in Weanling Pigs Experimentally Infected with a Pathogenic E. coli
Authors: Kim Kwangwook , He Yijie , Jinno Cynthia , Yang Seijoo , Li Xunde , Bravo David , Cox Eric , Liu Yanhong
Published: May 2021

The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Growth performance was measured on d -7 to 0 before inoculation, and d 0 to 5 and 5 to 11 post-inoculation (PI). Diarrhea score (DS; 1, normal, to 5, watery diarrhea) was daily recorded for each pig. Fecal samples were collected on d 2, 5, 8, and 11 PI to test the percentage of β-hemolytic coliforms in total coliforms. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Inclusion of oligosaccharide-based polymer linearly increased (P < 0.05) ADFI on d 0 to 5 PI, and feed efficiency on d 0 to 5 PI and d 5 to 11 PI (P = 0.07), compared with the control. Supplementation of AGP or oligosaccharide-based polymer reduced (P < 0.01) frequency of diarrhea of pigs from d 0 to 11 PI. No differences were observed in overall growth performance and percentage of fecal β-hemolytic coliforms on d 8 PI among pigs in AGP and oligosaccharide-based polymer treatments. In conclusion, supplementation of oligosaccharide-based polymer enhanced feed efficiency and reduced diarrhea of weaned pigs infected with a pathogenic E. coli.
188 Evaluation of Alternative Summer Feeding Strategies to Optimize Performance in High Lean Genetics Using Energy, Narasin and Phytonutrient Blend Individually, Or, in Combination
Authors: Knopf Brandon , Hanson Andrea , Silva Gustavo , Peterson Beau A , Soltwedel Kevin , Bible Megan , Sandberg Fredrik
Published: May 2021

The objective of this study was to determine the effects of feeding medium energy diets together with feed ingredients (Narasin-Skycis, Elanco Animal Health, Greenfield, IN; Phytonutrient blend-LeanFuel, Furst-McNess Company, Freeport, IL) as compared to low and high energy diets in high lean genetics during the summer. A total of 1375 pigs (PIC 337×PIC 1050; average 32.92 kg) were used in the experiment and were randomly assigned within block to 1 of 5 treatments by weight and gender, and 5 diet phases were formulated to meet and exceed NRC 2012 requirements, and formulated to a constant Lysine:NE ratio using corn, soybean meal and fat as low-energy, 3307 kcal/kg ME (L), moderate-energy, 3362 kcal/kg (M) and high-energy, 3417 kcal/kg ME (H) diets, and the trial ran from 32.92 kg to slaughter. Treatments 1) L 2) H 3) M with 1135 g/ton of LeanFuel added from 81.65 kg onwards 4) M with 13.6 g/ton Narasin throughout 5) M with 13.6 g/ton Narasin throughout and LeanFuel added from 81.65 kg onwards. Data were analyzed using the PROC GLIMMIX of SAS with pen serving as the experimental unit and blocks included in random effects. There was no difference in removals (P = 0.934). There was no difference in ADG between L and H, but H improved G:F by 2.5% (P < 0.01) as expected. ADG was intermediate for M+LF and M+Nar, with M+Nar+LF having a significantly greater ADG than both L and H by 4.8% for the overall period (P < 0.01). Feed efficiency was significantly lower for H, M+LF, M+Nar and M+Nar+LF as compared to L, but there was no difference for G:F between H and M+LF, M+Nar and M+Nar+LF. Gain per pig was increased by M+Nar+LF by 5.1 kg and 4.5 kg over L and H, respectively (P < 0.01). The combined use of Nar and LF offer an approach to supporting growth rate during the summer.
247 The Effect of Feeding Low Complexity Diets Contaminated with Deoxynivalenol and Supplemented with Nutramixtm or Fish Oil on Nursery Pig Growth Performance
Authors: Lariviere Elise Lafleur , Huber Lee-Anne , Zhu Cuilan
Published: May 2021

Three hundred twenty newly weaned pigs (6.7±0.3 kg BW) were used to determine the effect of low complexity diets contaminated with deoxynivalenol (DON) and supplemented with NutraMixTM or fish oil on nursery pig growth performance. Pigs were randomly divided into 40 pens and assigned to 1 of 5 dietary treatments (n = 8): [1] high-complexity diet containing animal proteins (HC) or one of four low complexity diets with protein supplied only by corn and soybean meal with [2] no DON contamination (LC), or [3] DON contamination of 3 ppm without supplements (DON-), [4] with NutraMixTM supplementation (2 g/kg; DONNM), or [5] with fish oil supplementation (2.5%, as-fed; DONω3). Diets were fed over two phases (7 and 15 days, respectively) and a common phase III diet was fed to all pigs for 20 days. In phase I, ADG, ADFI, and G:F were not different between pigs fed the HC and LC diets, but were lower for pigs fed DONNM and DONω3(P < 0.05). In phase II, pigs fed the DON- and DONω3 diets had lower ADG than LC (375 vs. 410 g/d; P < 0.05) and lower ADFI than HC (452 vs. 519 g/d; P < 0.05), while pigs fed DON- and DONω3 had greater G:F than those fed HC (0.83 vs. 0.78; P < 0.05). The BW at the end of phase II were not different between HC and LC (13.0 kg), but tended to be less for DONω3 (12.6 kg; P = 0.084 and 0.079, respectively). In phase III and over the entire nursery period, there were no treatment effects on ADG, ADFI, G:F, or final BW (26.0±0.7 kg). Feeding low complexity diets contaminated with 3 ppm DON initially reduced growth performance, but pigs were still able to achieve BW not different from HC pigs at the end of the nursery period, regardless of supplementation.
264 Effects of Low Dietary Crude Protein Diets Containing Coarse Wheat Bran as an Alternative to Zinc Oxide in Nursery Pig Diets
Authors: Hammers Kelsey , Calderon Hilda I , Tokach Mike D , Woodworth Jason C , Goodband Robert D , Dritz Steve S , DeRouchey Joel M
Published: May 2021

An experiment was conducted to determine the effect of reduced crude protein (CP) in diets containing coarse wheat bran (CWB) without pharmacological levels of Zn (ZnO) on growth performance and fecal dry matter (DM) of nursery pigs. A total of 650 pigs (DNA 241′600; initially 6.6 kg), were used with 5 pigs/pen and 26 replicate pens/treatment. Pens were assigned to 1 of 5 treatments in a RCB design based on BW. Treatments were fed for 13-d, contained 4% CWB, and consisted of: 1) diet with pharmacological ZnO (2,000 mg/kg Zn) and 21% CP formulated to 1.35% standardized ileal digestible (SID) Lys; 2) a diet with 110 ppm added Zn and 21% CP (1.35% SID Lys); 3) a diet with 110 ppm added Zn formulated to 18% CP (1.20% SID Lys); 4) an 18% CP diet with 110 ppm added Zn formulated to 1.35% SID Lys by the addition of increased levels of feed grade amino acids; and 5) diet 4 with addition of non-essential amino acids (NEAA; glycine and glutamic acid). Data were analyzed using the lmer function in R. Overall, pigs fed 21% CP with ZnO had increased (P < 0.05) ADG compared to those fed 18% CP (1.35% SID Lys) with high levels of feed grade amino acids or those fed the reduced SID Lys (1.2%) diet. Gain-to-feed ratio was increased (P < 0.05) for pigs fed 21% CP diets and those fed the 18% CP diet with NEAA compared to pigs fed 1.2% SID Lys and pigs fed high levels of feed grade amino acids. Fecal DM was increased (P < 0.05) for pigs fed reduced SID Lys diet. In conclusion, reducing CP (subsequently SID Lys) in diets without ZnO decreased growth performance but increased fecal DM.
266 Growth Performance, Fecal Score, and Blood Immune Parameters of Nursery Pigs Challenged with Escherichia coli F18 Fed Canola Meal-based Diet
Authors: Hong Jinsu , Ariyibi Samuel , Scaria Joy , Dilberger-Lawson Steven , Francis David , Woyengo Tofuko A
Published: May 2021

Effects of dietary canola meal (CM) on growth performance, fecal score, and blood parameters of E. coli-challenged nursery pigs were investigated. Thirty-six pigs (initial body weight = 6.22 kg) weaned at 21 d of age were housed individually and fed 3 diets (12 replicates/diet) in randomized complete block design. The diets were corn-soybean meal-based diet without or with antibiotics (0.2% chlortetracycline and 0.2% tiamulin) or with CM (20%). The diets were fed in 2 phases; Phase 1: d 0 to 7, and Phase 2: d 7 to 21. Pigs were orally challenged with F18 strain of E. coli on d 7. Feed intake and body weight were measured on d 7, 14, and 21. Blood samples for measuring thyroid hormones were collected from each pig on d 7, 14, and 21. Blood samples for measuring white blood cells, immunoglobulins, and cytokine were collected on d 21. Fecal score was assessed daily. Dietary antibiotics increased (P < 0.05) ADG by 50%. Dietary SECM also increased (P < 0.05) ADG by 23%. Week 1 fecal score was unaffected by diet. However, during weeks 2 and 3, fecal score for diet with antibiotics was less (P < 0.05) than that for basal diet or diet with CM, which did not differ in fecal score. During weeks 1 and 2, serum tetraiodothyronine level for diet with antibiotics was less (P < 0.05) than that for basal diet or diet with CM, which did not differ in serum tetraiodothyronine level; week 3 serum tetraiodothyronine level was unaffected by diet. White blood cell count was reduced (P < 0.05) by dietary antibiotics, and tended to be reduced (P = 0.087) by dietary CM. In conclusion, dietary CM increased ADG and tended to reduce white blood cell count. Thus, dietary CM at 20% may improve the performance of pigs challenged with E. coli F18 partly by decreasing immune response.
108 A Veterinarian’s Perspective on How Health and Nutrition Intersect
Authors: Hough Sara D
Published: May 2021

Nutritionists and veterinarians have long recognized that the provision and consumption of all essential nutrients is critical to the health of pigs, in all production phases. Animals that are properly fed, and maintain adequate body stores, are better able to reach their full genetic potential and defend themselves from pathogen invasion. This may be due to better tissue integrity, proper antibody production, improved detoxifying ability, and many other factors (Shurson et al, 1998). The pathology of various infectious organisms can influence absorption and the metabolism of nutrients, and inadequate nutrition can worsen the frequency, duration or pathogenicity of infectious disease. Herd health programs can only be most effective if pigs have adequate nutrition. Although much attention has been given to diagnostics, immunology, epidemiology and surveillance of infectious challenges, there is still relatively little known about the interactions viruses have with digestibility, metabolism and tissue accretion (Gabler et al, 2017). There is increasing interest in discovering ways to support the immune system, as improved alternatives are necessary with evolving restrictions on antibiotic use, zinc and others. The future demands a holistic approach to create resilient, robust animals, and that we identify alternatives that improve the productivity and overall wellbeing of food animals. It is well established that the gastrointestinal tract is the largest interface between the external and internal environments of the pig, and that the pig gut microbiota plays a critical role in maintaining metabolic homeostasis in harmony with a myriad of other physiological functions. Knowing this, how do we “feed the gut” and support a proper microbiome? What considerations do we need to have for the wean pig, the growing pig, the often-forgotten replacement gilt, and the challenges and environments they encounter? In this presentation, we will describe the relationships of nutrition and health, and how they can influence productivity.
107 Raised Without Antibiotics, Lessons Learned
Authors: Lincoln Whitney
Published: May 2021

Smithfield Foods has initiated Raised Without Antibiotics (RWA) programs into its vertical integration model in the past. This opportunity to drive change to meet a growing demand provides an unique ability to analyze approaches to raising swine in a setting where multiple entities are tied to the success of a program and maintaining a focus of animal welfare, traceability and financial responsibilities are a must. There is a wide range of housing and resources used for industry RWA programs across those that implement such practices. Smithfield Foods program was performed in conjunction with its company-owned sow farms weaning to contracted grower bases in conventional wean-to-finish barn settings within the Midwest making it a multi-entity managed approach. Therefore, a full-package view is mandatory for the success of a RWA program and takes into account many current industry research approaches as well as fulfilling basic husbandry parameters. A focus too heavily on one will alter the ability to achieve program goals. The focus in the most recent success of the RWA program provided by Smithfield Foods included beginning with high health pig sources sourcing robust offspring, utilizing strategic vaccines and timing to combat potential obstructive issues, specific biosecurity plans, optimal environment uniformity, nutrition and water quality focuses and individual pig awareness throughout the entirety of the pig’s time in the program. Over 400,000 pigs were placed into Smithfield Foods RWA Program. The outcome of implementing the multi-faceted approach of securing the basic animal husbandry, along with newer industry knowledge and research, led to only 1.53% individual pigs needing to be taken off of the program due to requiring an antibiotic treatment and the remaining pigs within the program having high success in financial returns with competitive company growth performances.
103 The Role of Crude Protein in Reducing the Need for Antibiotics in the Post-weaning Period
Authors: Pluske John
Published: May 2021

For many decades, antimicrobial compounds such as antibiotics and some mineral compounds have been used in pork production to promote pig growth and survival after weaning through mitigation of subclinical and clinical diseases, such as enterotoxigenic strains of Escherichia coli. Bans and restrictions in the use of some antimicrobial compounds has led, often out of necessity, to the development of alternative feeding strategies to address these challenges. One tool to reduce the post-weaning malaise in the absence of specific antimicrobial compounds is manipulation of the protein content of diets offered to pigs. Numerous studies have shown that feeding higher crude protein diets in the post-weaning period, usually in the absence of specific antimicrobials, is generally related to decreased fecal consistency (looser stools) and an increased incidence of post-weaning diarrhea. In some cases, this causes more therapeutic antibiotic administrations, more animal care, and a greater mortality. Production indices are poorer as a consequence. Conversely, feeding diets of lower crude protein content after weaning has typically been associated with better health outcomes, but if diets are not correctly formulated with the addition of appropriate crystalline amino acids, then production outcomes can suffer. Increasing the quantity of fermentable protein entering the cecum and colon by feeding more dietary protein will stimulate changes in microbial composition, with the microbiota shifting to a more N-utilizing community resulting in greater protein catabolic activity and the increased production of products including ammonia, amines and branched-chain fatty acids. This, in turn, can be associated with increased diarrhea. Formulating starter diets that limit the flow of exogenous and endogenous proteinaceous compounds posteriorly to the ileo-caecal valve can be used to reduce the post-weaning malaise and may minimize the use of certain antimicrobial compounds. However, feeding lower-protein diets alone is not always the panacea to this production issue, and will likely need to be used in conjunction with other targeted nutritional and health measures specific to the farm in question.
109 When Too Many Feed Additives Is Not a Sustainable Approach to Replacing Feed Antibiotics
Authors: Bradley Casey L , Rosero David
Published: May 2021

Due to consumer and regulatory demands swine producers are being ever more pressured to remove feed- and water-grade medications from their production systems. Consequently, there is an ever-growing list of feeding strategies and feed additives that indicate their value to maintain growth performance in diets void of antibiotics, leaving a confusing landscape for producers to navigate their options. Non-antibiotic feed additives, as reviewed by Liu et al. (2018), of options include acidifiers, minerals, prebiotics, direct fed microbials or yeast, nucleotides, and plant extracts. Furthermore, enzymes such as phytase and xylanase when fed to pigs have shown indirect benefits to not only performance but also livability. But within this landscape of research there has been little evidence to support synergies of these different feed additive types as an opportunity to replace antibiotics, while providing relatively little on the economic implications of different strategies. A commercial example of this was a study conducted in the Hanor System (Enid, OK USA) in which they conducted a 3 different feed additives that could benefit gut health of nursery pigs: organic acids, phytochemicals, and direct-fed microbials. Of the three different products tested, the phytochemical additive was the only product to demonstrate a profit above diets without the addition of feed additives. Furthermore, another study in the Hanor System evaluating antibiotic alternatives in the nursery period using pigs that were positive with PRRSv (1-7-4) found an increase in mortality and reduction in full value pigs when antibiotics were removed. However, the beta-glucan product demonstrated a positive response to both mortality and full-value pigs, yet still did not return to full profitability as the antibiotic fed pigs. In conclusion, further investigation into the synergies of different categories of feed additives is necessary to not only find the ideal balance of performance benefits but also profitability.
262 Fiber and Co-product Utilization in Pigs
Authors: Zijlstra R T T , Beltranena E
Published: May 2021

Fiber is one of the four macronutrients that yield dietary energy for pigs (others are starch, fat, and protein). To yield energy, fiber must be fermented (primarily in the hindgut) by microbes producing volatile fatty acids (VFA). Price increases for traditional feedstuffs such as cereal grains and protein meals have stimulated the pork industry to consider dietary inclusion of fibrous co-products that are produced when grain is processed into human food, fuel, and bio-industrial products. High fiber co-products include distillers dried grains with solubles (DDGS), oilseed meal, expeller, and cake, and co-products from flour milling. As omnivores, pigs are ideally suited to convert these non-human edible co-products into high quality food animal protein. Thereby, co-products can partially offset increases in feed cost provided their price is competitive per unit of net energy or digestible lysine, but also present risks and feeding challenges. Effects of feeding high fiber co-product may depend on diets being balanced for energy value or not. In weaned pigs, high fiber diets were thought to reduce feed intake, and thereby hinder energy intake during the energy-dependent phase of growth. However, such a relation is not solid across the spectrum of dietary fiber. Fiber characteristics play a role in gut health and early development. High fiber diets have lower energy digestibility and concurrent lower feed efficiency, but increased feed intake may maintain growth. In growing-finishing pigs, high fiber diets increase viscera mass, and thereby reduce dressing percentage. In restricted-few sows, fiber and produced VFA play a role in reaching satiety responses. In conclusion, depending on the price of high fiber co-products, high fiber diets may be part of a range of solutions to reduce the feed cost, and may thereby support economically-sustainable pork production.
263 Review of Current Nutrition Knowledge and Practices for Gilt Development
Authors: Menegat Mariana , Tokach Mike D
Published: May 2021

Nutrition for replacement gilts aims to support the development of prepubertal gilts towards their physiological maturity in terms of body weight, body tissue composition, structural soundness, and reproductive development. A key concept of gilt nutrition for lifetime productivity is to maintain a positive prepubertal growth rate and reach a target body weight of 115 to 140 kg at puberty. The application of this concept commonly presents a challenge with fast-growing and highly efficient contemporary gilt lines, particularly considering the proportion of gilts exceeding the target body weight at puberty and the negative impacts of overweight gilts on lactational performance, structural soundness, and longevity. Thus, nutritional decisions regarding dietary levels of energy, amino acids, and minerals in the pre- and peripubertal stage can be conflicting. Typically, gilts are fed ad libitum with moderate levels of energy and amino acids because restrictions below the requirements can have a negative effect on puberty onset. In addition, a high plane of nutrition is offered after the pubertal estrus to set ovulation rate for breeding at the next estrus. Moreover, gilts are fed levels of dietary calcium, phosphorus, and trace minerals above the requirements to improve bone mineral density in preparation for fetal development and lactation mobilization. Although these nutritional practices improve gilt performance, they also typically increase weight gain. Thus, there is a need to review nutritional strategies to manage body weight of replacement gilts while attaining optimum reproductive success and lifetime productivity.
261 Dietary Fiber: Chemical and Physical Characteristics and Methods of Analysis
Authors: Fahey George C
Published: May 2021

The fiber component of the diet is the major food source for the intestinal microbiota of swine. Included are traditional insoluble (e.g., cellulose) and soluble (e.g., pectin) dietary fibers, resistant starches (four types), and oligosaccharides (some of which are “prebiotics”). Fiber constituents are found both in the primary and secondary cell walls of plants, but some are found in non-cell wall structures as well. In cereals, cell walls consist of a reinforced multi-component matrix of cross-linked polymers (acidic xylans, arabinoxylans, glucomannans) in which a network of cellulose microfibrils is embedded. Proteins form a second network in the matrix, and lignin and phenolic acids may be covalently linked to the matrix polysaccharides. In addition, whole grains have considerable amounts of resistant starch. Physical processing and cooking have a major effect on the chemical composition and subsequent utilization of the whole grain. Techniques are available to quantify both the insoluble and soluble fibers present in swine diets. Solubility often impacts the location of fermentation within the swine gastrointestinal tract, and solubility often, but not always, reflects fermentation potential. Other important attributes of fiber include its viscosity/gel-forming capacity/water-holding capacity, and its fermentability. On the analytical front, considerable progress has been and continues to be made, with many AOAC-approved techniques currently available. In summary, dietary fiber is perhaps the most chemically complicated of any swine feed constituent, making its analysis difficult. Fibers, whether they be intrinsic and intact, isolated, chemically and (or) enzymatically synthesized, alternative, etc.represent the key foods for the swine intestinal microbiota, so a good understanding of their properties is critical to fully understand how to optimize their role in swine nutrition.
79 Direct and Indirect Effects of Heat Stress on the Hepatic and Ovarian Proteome in Gilts
Authors: Studer Jamie M , Kiefer Zoe E , Gianluppi Rafael , Adur Malavika K , Schultz R Blythe , Horst Erin A , Mayorga Edith J , Keating Aileen F , Baumgard Lance H , Lents Clay A , Ross Jason W
Published: May 2021

Seasonal infertility (SI) caused by heat stress (HS) impacts the US swine industry by reducing litter size, farrowing rates, and production efficiency. Identifying the biological underpinnings of SI is a foundational step towards developing mitigation strategies to reduce the nearly $1 billion annual revenue losses to the swine industry. The study objective was to investigate the direct and indirect effects of HS via HS conditioned serum infusion in swine. We hypothesized gilts housed in thermoneutral (TN) conditions receiving gradual infusions of serum obtained from HS gilts would experience altered endocrine and metabolic function compared to gilts receiving serum from TN gilts. Prepubertal gilts (n = 18) were assigned to donor or recipient groups and donors were allocated to TN or HS environments. Blood was collected from HS donors and TN donors exposed to 24-hours of HS or TN conditions, respectively. Serum was infused into recipients housed in TN conditions via indwelling jugular catheters. Over a 24-hour period approximately 20% of the estimated recipient gilt serum volume from donors (pooled by treatment) was infused into recipients. After infusions were completed, gilts were euthanized and tissues collected. Increased rectal temperatures were observed in HS recipients compared to TN recipients (P ≤ 0.05). Protein extracts from liver and ovary underwent proteomic analysis via liquid-chromatography with tandem mass-spectrometry (LC-MS/MS) to assess protein abundance. In the liver and ovary, we identified 135 and 264 proteins, respectively, that were differentially abundant between TN and HS recipients (P < 0.10). Gene Ontology enrichment analysis identified alterations to pathways involved in hormone regulation, immune response, and apoptosis. Collectively, these data demonstrate gilts receiving HS serum experienced altered endocrine and metabolic function compared to gilts receiving TN serum. This project was supported in part by the Iowa Pork Producers Association. USDA is an equal opportunity provider and employer.
82 Methyl Donor Supplementation Alters Cytosine Methylation and Biological Processes of Cells Cultured in Divergent Glucose Media Reflecting Improvements in Mitochondrial Respiration and Cell Growth Rate
Authors: Crouse Matthew S , Da Silva Diniz Wellison Jarles , Caton Joel , Dahlen Carl R , Reynolds Lawrence P , Borowicz Pawel P , Ward Alison K
Published: May 2021

We hypothesized that supplementation of one-carbon metabolites (OCM: methionine, folate, choline, and vitamin B12) to bovine embryonic tracheal fibroblasts in divergent glucose media would alter cytosine methylation, and alterations in cytosine methylation will reflect biological processes matching previously improved mitochondrial respiration, cell proliferation, and cell growth rate data. Cells were cultured with 1g/L glucose (Low) or 4.5g/L glucose (High). Control medium (CON) contained basal concentrations of folate (0.001g/L), choline (0.001g/L), vitamin B12 (4µg/L), and methionine (0.015g/L). The OCM were supplemented at 2.5 and 5 times (2.5X and 5X, respectively) the CON media, except methionine was limited to 2X across all supplemented treatments. Cells were passaged three times in their treatment media before DNA extraction. Reduced representation bisulfite sequencing was adopted to analyze and compare the genomic methylation patterns within and across treatments using edgeR. Biological processes (BP) were retrieved based on the nearest genes of differentially methylated cytosines (P < 0.01) for each comparison between treatments. In both Low and High treatments, greater OCM increased the proportion of hypomethylated vs. hypermethylated cytosines. Functional analyses pointed out positive regulation of BP related to energy metabolism, except for the contrasts within the High group. Among the BP, we can highlight positive regulation of: GTPase activity, catalytic activity, molecular function, protein modification processes, phosphorylation, protein phosphorylation, cellular protein metabolic processes, MAPK cascade, and metabolic processes. These data support previously reported results from this experiment that showed increased mitochondrial respiration, cell proliferation, and growth rates with increasing OCM levels. We interpret these data to imply that when energy and OCM requirements are met for growth and basal methylation levels, DNA methylation levels decrease which may allow for greater transcription. Thus, OCM can be utilized for other functions such as polyamine synthesis, nucleotide synthesis, energetic metabolites, and phosphatidylcholine synthesis. USDA is an equal opportunity provider and employer.
259 Effects of Saccharomyces Cerevisiae Fermentation Products on Lactating Sow’s Blood and Uterine Cytokine Profiles
Authors: Garcia Ricardo M , Thayer Morgan T , Mills Kayla M , Richert Jacob A , Sharp Katharine G , Rulon Karissa N , Petry Derek B , Robertson Karen , Stewart Kara R , Radcliffe John S , Schinckel Allan P , Richert Brian T
Published: May 2021

This study evaluated the effects of a liquid prototype (LIQP) and dry (XPC®; Diamond V) Saccharomyces cerevisiae feed additives on blood and uterine cytokine profiles in sows (n = 40). On d 112 of pregnancy sows were allotted to dietary treatments: 1) Control diet (CON), 2) CON +15 mL of LIQP (LIQ), 3) CON +0.20% of XPC (DRY), and 4) DRY +15 mL of LIQP until d 7 post-farrowing (D+L). Interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were quantified from serum samples collected on d 112 of gestation, d 2 and 6 post-farrowing, and from uterine fluid collected on d 2, 4, and 6 post-farrowing. Serum C-Reactive protein (CRP) and haptoglobin concentrations were evaluated. No interactions between treatments and day of collection were observed (P > 0.13). LIQ and D+L sows had the greatest serum IL-10 concentration (P < 0.001) and sows fed CON tended to have lower concentration of IL-8 (P < 0.06) vs. other treatments. Serum CRP concentrations were greatest on d 2 (P < 0.001), serum IL-10 (P < 0.04) and IL-4 (P < 0.07) linearly decreased while serum haptoglobin (P < 0.02) and INF-γ (P < 0.001) linearly increased post-farrowing. In the uterine fluid, LIQ and D+L sows had greater INF-γ (P = 0.04) concentrations and CON tended to have the least concentration of TNF-α (P = 0.08). Uterine fluid IL-1 tended to linearly increase (P < 0.07) and IL-6 linearly decrease (P < 0.01) post-farrowing. No strong correlations were detected between cytokines in the serum and uterine fluid within day. LIQ sows had the greatest daily feed intake and CON the least during the first week of lactation (P = 0.04). Providing LIQP post-farrowing to sows modified immune response increasing both pro- and anti-inflammatory cytokines in serum and uterine fluid in an independent manner, allowing animals a quicker recovery and increased feed intake.
287 Effects of Number of Sperm and Site of Uterine Semen Deposition on Conception Rate and Number of Embryos in Weaned Sows Receiving a Single Fixed Time Insemination
Authors: Stewart Kara R , Belstra Brad , Willenburg Kilby L , Gómez-López Domingo , Knox Robert V
Published: May 2021

Induced ovulation with single fixed time artificial insemination (SFTAI), combined with uterine (IUI) or deep uterine insemination (DUI), could improve fertility with low numbers of sperm and allow greater use of high genetic merit boars. At weaning (0 h), sows (n = 534) were assigned by parity and estrus induction method (eCG or Control) to receive 1200 × 106 sperm by IUI, 600, 300, or 150 × 106 sperm by IUI or DUI, or 75 × 106 sperm by DUI. At 80 h post weaning, sows received OvuGel and 26 h later a pooled semen SFTAI. Ultrasound was performed to determine follicle size and time of ovulation (OV). Sows were slaughtered 27 d after AI to determine pregnancy and litter traits. There was no effect (P > 0.05) of eCG on estrus (93%) within 5 d of weaning or follicle size (6.1 mm) at OvuGel, but wean-to-estrus (3.8 vs. 4.0 d,P < 0.01) and AI-to-OV (15.9 vs. 17.0 h, P = 0.04) intervals and AI-to OV were slightly reduced. eCG did not affect pregnancy rate (78.6%), number of CL (21.7), or number of viable embryos (12.2). There was no effect of number of sperm or site of insemination (P > 0.05) on pregnancy rate (range: 80.9% to 70.5%), but AI occurring after ovulation reduced pregnancy rate (P < 0.02). Total number of embryos (range: 16.5 to 10.3) increased with CL number (P < 0.001) but was not affected by number of sperm or site of insemination (P > 0.05). Higher sperm treatments (1200 and 600 x 106) had more embryos compared to lower sperm treatments (P < 0.01), suggesting that lower sperm numbers effects litter size more than the pregnancy status. Acceptable fertility can be achieved with low sperm numbers when using SFTAI and uterine deposition, but AI-to-OV interval and ovulation rate influence final fertility.