bluuebunny/crossref_metadata_2025 huggingface dataset:
Family Comes First: An Investigation on Entrepreneurial Intention among Chinese Indonesian Gen Z
Authors: Kusumawardani Kunthi Afrilinda , Richard Richard
Published: September 2020

Family and education have often been studied by numerous researchers as the factors of entrepreneurs’ creation. The research discusses the influence of background of family and education in entrepreneurship on the entrepreneurial self-efficacy in shaping the entrepreneurial intention among Chinese Indonesian Gen Z. Through the application of the Theory of Planned Behaviour (TPB), this research employed quantitative method design. This current study uses Structural Equation Modelling (SEM) analysis of 202 individuals. The finding indicates that Family Background and Entrepreneurship Education affect Entrepreneurial Self-Efficacy, and Entrepreneurial Self-Efficacy mediates the relationship between Family Background and Entrepreneurship Education in Entrepreneurial Intention. However, Family Background and Entrepreneurship Education do not have direct influences on Entrepreneurial Intention. Based on the finding, it can be determined that Family Background and Entrepreneurship Education will stimulate a high Entrepreneurial Self-Efficacy which leads to Entrepreneurial Intention. Thus, this study suggests the stakeholder in education in Indonesia create an entrepreneurship-based system in the education curriculum to equip students with knowledge of entrepreneurship and develop the skills not only to discover the business opportunity, but also to create a sustainable one, and at the same time encouraging them to have self-confident in their capability in becoming thriving entrepreneurs. While the finding on Family Background signify that entrepreneurs are nurture, not nature since they need to be stimulated along with Entrepreneurship Education and Self-Efficacy. The limitation of this study lies in the size of the sample, thus future research is recommended to take a larger sample to get more representable data to envisage the Entrepreneurial Intention. This study provides an insight into the Entrepreneurial Intention among Chinese Indonesian Gen Z which has never been investigated in the past. Additionally, it contributes the empirical evidence of Entrepreneurial Self-Efficacy role in transforming Family Background and Entrepreneurship Education into Entrepreneurial Intention.
Family Business Sustainability through Community Orientation
Authors: Rahmiati Filda
Published: September 2020

This research aims to explore the sustainability of family business through the implementation of Corporate Social Responsibility (CSR) to the community surrounding the company. As many research studies mentioned that family businesses are often strongly anchored in the local community, the high commitment to the local community will leads to a high degree of socially responsible acting. Qualitative method is used in the form of a case of an Indonesian small business in Bogor. The data collection is done using direct methods like observation, interview, and going through the company documentations to comprehend the case situation. The result of the research shows that the CSR implementation, in case observed, was in accordance with the needs of surrounding community. The CSR activities were in the form of the education, religious activities, public facilities, employee and community empowerment. The community and the company were supporting each other in order to make the activity success. This study concludes that this symbiotic CSR could be new model for family business enterprises sustainability through CSR.
Krakowski katalog dobroczyńców zakonu bernardynów 1453–1602. Z dziejów księgozbiorów zakonnych
Authors: Szelińska Wacława
Published: September 2020

Autorka omówiła krakowski katalog dobroczyńców zakonu bernardynów z lat 1453–1602 znajdujący się w zbiorach rękopiśmiennych Archiwum Prowincji Bernardynów w Krakowie. Wykazała, że katalog jest cennym źródłem do badania procesu tworzenia i narastania księgozbioru krakowskich bernardynów oraz do poznania kręgu ludzi z tym związanych. Choć część darowanych ksiąg nie miała zbyt dużej wartości merytorycznej, to legaty na rzecz biblioteki zyskiwały na znaczeniu z uwagi na osoby darczyńców. Były to przede wszystkim osobistości naukowego Krakowa – profesorowie, rektorzy oraz inny uczeni.
Potrzeby badawcze w zakresie bibliotek szkolnych i pedagogicznych
Authors: Drzewiecki Marcin
Published: September 2020

Autor przedstawił wnioski z prowadzonych przez siebie analiz działalności bibliotek szkolnych i pedagogicznych w Polsce. Wskazał na konieczność prowadzenia badań w zakresie funkcjonowania tych bibliotek w procesie dydaktyczno-wychowawczym szkoły i ich miejsca w krajowej sieci biblioteczno-informacyjnej. Zaproponował też przeprowadzenie zmian organizacyjnych, strukturalnych i funkcjonalnych w sieci bibliotek szkolnych i pedagogicznych, a także zaapelował o zmiany w kształceniu nauczycieli i bibliotekarzy szkolnych.
Peran BPSK Dalam Menyelesaikan Sengketa Konsumen
Authors: Haryani Anik Tri , Sarjiyati Sarjiyati , Purwati Yuni
Published: September 2020

Abstract— The purpose of this study is to analyze and explain the role of BPSK in resolving consumer disputes and analyze the dispute resolution process BPSK. This research used normative juridical approach to the law (statute approach) and the conceptual approach (conceptual approach). The results showed that in the implementation of consumer protection in Indonesia, BPSK is spearheading role in the field to provide protection to consumers who have been harmed. The protection afforded by BPSK to consumers is through the settlement of disputes between consumers and businesses, and also through the inclusion of supervision of any agreement or document that outlines the standard clause. While the dispute settlement process is done for the sake of the stages of the stages are as follows: stage request of the applicant, ie the consumer as plaintiff; pretrial stages, namely the selection of completion method; settlement of disputes based on the decision of the parties, especially mediation, conciliation, arbitration and the decision of the Assembly. Keywords—: Role; BPSK; consumer disputes.
Akateemiset generalistit vuosituhannen vaihteen työmarkkinoilla
Authors: Rouhelo Anne , Rautakilpi Sirkku
Published: September 2020

Generalistialoilta valmistuneilla oman paikan etsiminen on ongelmallista, koska heidän tutkintonsa ei johda suoraan tiettyyn ammattiin, vaan koulutusta on osattava soveltaa eri alueille. Ennen 1990-luvun alun syvää lamaa akateemisten generalistien työura oli kuitenkin taattu. Artikkelissa kuvatun tutkimuksen perusteella on löydettävissä viisi erilaista generalistien työuratyyppiä: pysyvä, nousujohteinen, liikkuva, katkonainen ja yrittäjyyshenkinen työura.
Ammattikorkeakoulun oppimisverkostossa kehittyy uudenlaista oppimista
Authors: Lambert Pirjo , Reunanen Raija , Helle Merja
Published: September 2020

Verkostoitunut toiminta nostaa esille kysymyksen opitun siirtovaikutuksesta. Miten opitut tiedot ja taidot siirtyvät eri kulttuurikontekstien välillä? Artikkelissa sovelletaan kehittävän siirtovaikutuksen mallia, missä merkittävää on vaakasuuntainen liike toimijoiden välillä. Ammattikorkeakoulun kokeilussa siirtovaikutuksia on seurattu ja etsitty opettajien ja opiskelijain välisestä vuorovaikutuksesta sekä erilaisista opiskelijain kirjoitustöistä, kuten kirjallisista kuvauksista ja opinnäytetöistä.
Analyysi kehittämishankkeen keskusteluista
Authors: Korhonen Satu-Mari
Published: September 2020

Mitä enemmän asioita yritetään muuttaa, sitä enemmän ne pysyvät ennallaan (Seymour Sarason 1971, 2).
Perinteiset muutosta ja kehittämistä koskevat teoriat lähtevät liikkeelle ajatuksesta, että muutos toteutetaan ylhäältä-alas (ks. Kotter 1996). Tällaiselle käsitykselle on ollut ominaista tulkita työntekijöiden rooli muutoksen toteutumista estävänä tekijänä. Käsitys nojautuu perinteiseen vastarinta-metaforaan. Työyhteisöä ja työtä on mahdollista kehittää osallistavallakin tavalla, jolloin työntekijät itse toteuttavat kehittämistyötä. Osallistavassa kehittämismenetelmässä kohdataan kuitenkin haaste, sillä työntekijöiden tulisi suhtautua reflektoiden omaan työhönsä. Mikäli tällainen oman toiminnan ”kriittinen sivusta seuraaminen” ei onnistu, voi tämä estää kehittelyä ja aiheuttaa pitäytymisen vanhassa. Tarkoituksenani on tutkia muutoskeskusteluja myyteistä käsin. Millaisia myyttejä keskusteluissa esiintyy? Mitä puheessa oikeastaan tapahtuu kun yhteinen työn kyseenalaistava tutkiminen ”pysähtyy”.
Kuhinaa sienimetsässä
Authors: Valkama Hannu
Published: September 2020

Ekspansiivinen oppiminen ja yhteiskehittely työssä, Yrjö Engeström, Tampere (2004)
Itsereflektio ja kohentunut vointi haastattelupuheessa
Authors: Vehviläinen Sanna , Lindfors Olavi
Published: September 2020

Itsereflektio on oman kokemuksen, toiminnan, tiedon tai asennoitumisen erittelyä. Sitä tarvitaan osana oppimisprosesseja, asiantuntijuutta ja terapeuttista muutosta. Terapisoituva kulttuuri ja useilla elämänalueilla kasvava vaatimus yksilön suorittamaan itsen muokkaamiseen ja itseä koskevien tulkintojen esittämiseen tekee itsereflektiosta tärkeän tutkimuskohteen aikuiskasvatuksen, psykoterapiatutkimuksen sekä sosiologian kannalta.
Työsuojeluinterventio Turun telakalla
Authors: Hallikainen Petri
Published: September 2020

Työskentely laivanrakennusteollisuudessa osana metalliteollisuutta on tilastollisesti tarkastellen yksi vaarallisimmista tavoista tienata leipänsä. Suomen suurin laivanrakennusteollisuuden työnantaja Kvaerner Masa Yards (nykyinen Aker Finnyards) aloitti vuonna 2000 uudenlaisen panostuksen työsuojeluun. Mitä hyötyä interventiosta oli ja mitä lopulta jäi käteen?
Keskusteluyhteyttä rakentamassa?
Authors: Tomperi Tuukka
Published: September 2020

Konstruktivismi ja realismi, toim. Pekka Kalli ja Anita Malinen, Helsinki (2005)
Yliopiston arviointiprosessit moraalisena sääntelynä
Authors: Koski Leena
Published: September 2020

”25 vuotta sitten pidettiin mielettömänä ajatusta yliopistojen arvioimisesta määrien ja aikojen perusteella, koska siten olisi sivuutettu tai unohdettu yksi yliopiston tärkeimmistä tehtävistä, ajattelun kehittyminen. Tänään pidetään mielettömänä olla niin tekemättä, koska siten sivuutettaisiin yksi yliopiston tärkeimmistä tehtävistä, tuottaminen.” Professori Leena Koski arvioinnin analyysiin keskittyneessä virkaanastujaispuheessaan 15.5.2005.
Oman avun yhteisö syrjäytymistä estävänä tekijänä : tutkimus vertaistukeen perustuvista ratkaisuista ja tuloksista
Authors: Vilmi Veikko
Published: September 2020

Tarkoituksenani on selvittää niitä menettelyjä, joiden avulla oman avun yhteisö voi vaikuttaa syrjäytymiseen estävästi. Esimerkkinä on kansainvälistä huomiota saavuttanut Kuopion Sirkkulanpuiston toimintayhteisö. Yhteisön toiminta muistuttaa osuuskuntatyyppistä taloudellista yhteistyötä. Artikkelin tutkimustulokset kuvaavat pyrkimyksiä edistää syrjäytymisvaarassa olevien henkilöiden hyvinvointia vertaistuen ja yhteistoiminnan avulla. Koulutuksen merkitys on oleellinen seikka syrjäytymistä estävien tavoitteiden toteuttamisessa.
Kuurojen ja huonokuuloisten opiskelijoiden kaksi koulutussukupolvea
Authors: Lehtomäki Elina
Published: September 2020

”Ruotsalaisten ja yhdysvaltalaisten tutkimusten mukaan kuurojen ja huonokuuloisten aikuisten koulutustaso, työllisyystilanne ja sosiaalinen asema ovat muuttuneet sukupolvesta toiseen siirryttäessä. Koulutus on ollut tärkeä tekijä sosiaalisen aseman muutoksessa. Näin ei vielä ole tapahtunut oppimisyhteiskunnan mallimaassa, Suomessa”, aiheesta väitellyt Elina Lehtomäki kirjoittaa.
Mikä onkaan suhteeni brändeihin?
Authors: Siirilä Jani
Published: September 2020

Brändätyt : ostetaan ja myydään nuoria, Alissa Quart, Helsinki (2003)
Mitä on suomalainen aikuiskasvatustutkimus?
Authors: Salo Petri
Published: September 2020

On vierähtänyt vuosikymmen siitä, kun suomalaisen aikuiskasvatustutkimuksen suuntauksia ja sisältöjä pohdittiin Aikuiskasvatuslehden sivuilla. Vuoden 1995 viimeinen numero omistettiin suomalaisen aikuiskasvatustutkimuksen tuolloisen tilan tarkastelulle. Hahmotan kyseisen teemanumeron suomalaisen aikuiskasvatustutkimuksen toisen vaiheen tilinpäätökseksi. Mutta mitä kolmas vaihe ja sille tunnusomainen vahva ekspansio on merkinnyt aikuiskasvatustutkimuksen sisältöjä ajatellen? Tarkastelen artikkelissa Aikuiskasvatuksessa julkaistuja tieteellisiä artikkeleita vuosina 1996–2004.
Supertohtoreita – miten niitä tehdään?
Authors: Pyhältö Kirsi , Soini Tiina
Published: September 2020

Tutkijankoulutuksesta käytävää keskustelua hallitsevat yhä määrällisiin kysymyksiin ja muotoseikkoihin liittyvät kysymykset. Koulutusprosessia ei ole juurikaan tarkasteltu oppimisen ja tutkimuksen näkökulmista. Tohtorin osaamisen ”toivelistasta” puuttuu miltei kokonaan opettamiseen liittyvä, koulutuksellinen ja konsultatiivinen osaaminen. Tarkastelemme tieteellistä jatkokoulutusta opetussuunnitelmatyön näkökulmasta. Viitekehyksenämme on kasvatuspsykologinen oppimisen tutkimus.
Sukupuoli aikuiskasvatuksen tutkimuskohteeksi
Authors: Vuorikoski Marjo , Ojala Hanna
Published: September 2020

Seuraava keskustelunavaus sukupuolesta suomalaisessa aikuiskasvatustutkimuksessa lähti liikkeelle omakohtaisesta ihmettelystä. Tunnemme joskus olomme hiukan yksinäisiksi aikuiskasvatustutkijoiden joukossa. Ihmettelemme, miksi meidän lisäksemme vain harvoja aikuiskasvatustutkijoita näyttää kiinnostavan sukupuoli, vaikka sen merkitys on alkanut saada yhä enemmän sijaa suomalaisessa kasvatustieteen ja työelämän tutkimuksessa. Päätimme tutkia, miten sukupuoli näyttäytyy esimerkiksi aikuiskasvatustieteen viroissa, alan väitöksissä tai Aikuiskasvatuksen tiedeartikkeleissa?
On the Trigonometric and p-Trigonometric Functions of Elliptical Complex Variables
Authors: ÖZEN Kahraman Esen
Published: September 2020

In the early 2000s, the geometry of a one-parameter family of generalized complex number systems was studied (Math. Mag. 77(2)(2004)). This family is denoted by Cp. It is well known that Cp matches up with the elliptical complex number system when p is any negative real number. By using this system, Özen and Tosun expressed the elliptical complex valued trigonometric functions cosine, sine and p-trigonometric functions p-cosine, p-sine (Adv. Appl. Clifford Algebras 28(3)(2018)). In this study, we introduce the remained elliptical complex valued trigonometric and p-trigonometric functions. Also we define the corresponding single-valued principal values of the inverse trigonometric and p-trigonometric functions by following the similar steps given in the literature.
SOSIALISASI DAN PENDAMPINGAN PENCEGAHAN PENYEBARAN COVID-19 DI PERUM TANGGAP COVID (PTC) AMERTA RESIDENCE RT 08/RW 05 DESA SAPTORENGGO
Authors: Hidayati Nuril , Prayitno Trio Ageng , Riyanto Riyanto
Published: September 2020

Saptorenggo Village is declared as a Covid-19 red zone. However, the villagers, especially the residents of Perum Amerta Residence continue to engage in outdoor activities  even some of them still work out of town. This behavior is a source of major problem in the distribution of Covid-19 at the Amerta Residence housing estate. A mutual agreement between the service team and the head of RT 08 and residents of the Amerta Residence housing estate to overcome the problem is needed so that the socialization and assistance in preventing the spread of Covid-19 can be performed. This type of activity is community service at the Amerta Residence RT 08 housing estate from March to June 2020. The procedure for community service activities consists of four stages including socialization through deliberations, the formation of the Perum Tanggap Covid (PTC) team, periodic disinfectant spraying assistance, and evaluation of community service activities. The results of this community service show that the community service team has succeeded in carrying out community service activities  base on the planned targets. In addition, residents of Amerta Residence housing estate can follow all the service activities that have been designed by the service team
Tinjauan Sosiolinguistik Keigo dalam Manga de Manabu Nihongo Kaiwa Jutsu
Authors: Kurniawati Safitri Indah
Published: September 2020

This paper discusses the use of Japanese keigo respectful types in terms of sociolinguistics. The purpose of this study was to determine the use of Japanese language respect in Nihongo Jaanaru in the Manga de Manabu chapter Nihongo Kaiwa Jutsu. This study uses data sources in the form of Nihongo Jaanaru in the chapter Manga de Manabu Nihongo Kaiwa Jutsu. In this study the discussion of the use of Japanese respect in the Nihongo Jaanaru chapter of the Manga de Manabu Nihongo Kaiwa Jutsu is examined using the distance theory framework proposed by Sachiko's Idea. This distance by Sachiko's idea is used to explain the sociolinguistic aspects of the Japanese respectful variety in Nihongo Jaanaru in the chapter Manga de Manabu Nihongo Kaiwa Jutsu. Based on the findings in the data analysis, the use of keigo is more emphasized on the distance of formality and psychological distance and keigo is used as respect and strategy to achieve the intended purpose.
Analisis Skema Aktansial dan Model Fungsional Greimas pada Cerita Pendek Tsuru no Ongaeshi
Authors: Santoso Budi , Soelistyowati Diah
Published: September 2020

AbstractA short story is one of the literary works that is not very long and generally focuses on a single event with one or two characters. This paper tries to describe the narrative structure in the story ãƒ„ãƒ«ã®æ©è¿”ã—. Source of data uses short story ãƒ„ãƒ«ã®æ©è¿”ã—, taken fromÂ  https://www.wasabi-jpn.com. The research approach uses structuralism especially Greimasâ€™s actantial schemeÂ  and the functional narrative model. The results show that the short story ãƒ„ãƒ«ã®æ©è¿”ã—Â  formed by one functional narrative model and 6Â  actual schemes. The functional narrative model consists of initial situation, transformation stage (qualifying test, main test, glorifying test), and the final situation. From 6 actan schemes, there are 4 complete actantial schemes and 2 incomplete actantial schemes (actantial schemes without opponent). There are 6 acting roles that make up the short story ãƒ„ãƒ«ã®æ©è¿”ã—;sender, receiver, subjects, objects, Â helpers,Â  and opponent .
Analisis Semantik Stilistika Gaya Bahasa dalam Lirik Lagu Hikaru Nara Karya Goose House
Authors: Bryantoro Reynardo Aaron
Published: September 2020

The title of this research is style of language of song lyrics hikaru nara by Goose House: a study of stylistic semantics. This research is aimed to analyze what are the meanings and kind of language style used on song lyrics hikaru nara by Goose House. The researcher uses descriptive qualitative method to analyze the meanings and kind of language style on song lyrics hikaru nara by Goose House by using theorys of stylistic semantics proposed by Simpson, theorys of style of langauge proposed by Yamanashi and theorys of meaning proposed by Ogden. The results show that the figures of speech used  in the song lyrics of hikaru nara are metaphor, personification, hyperbole, metonymy, and paradoks. Metaphor is the most dominant figure of speech used by the song lyrics writer. The meanings of the lyrics is after all the troubles faced by the writer in his life, finally he can be a better person and he believes his own strength. It can be said that hope and worries go together, it depends on how we achieve our destiny.Keyword : Style of language, Song lyrics, Stylistic semantics, Hikaru Nara, Goose House
Characterization and Antimicrobial Susceptibility of Pathogens Associated with Periodontal Abscess
Authors: Irshad Muhammad , Alam Mohammad Khursheed , Alawneh Ahmad , Alhadi Mohammed Abdullah , Alhadi Ahmed Abdullah , Almunajem Yasser Saleh , Alanezi Fesal Farag , Al Sagoor Sharafi Abdullah , Bajawi Abdulrahman Mudaysh , Alfawzan Ahmed Ali , Kamal Mohammad Amjad
Published: September 2020

Knowledge of microbial composition and antimicrobials’ susceptibility to periodontal abscesses is vital for their successful treatment. The current study aims to provide a thorough overview of the clinical and microbial features of periodontal abscesses of the local community. The study was carried out at Rehman College of Dentistry, Peshawar, Pakistan between December 2019 to March 2020. Clinical measurements and microbial samples were collected from 45 subjects. Microbial samples were anaerobically cultured for the growth of selected bacterial species. E-test was used to assess the susceptibility of bacterial species grown from the patient samples to amoxicillin, azithromycin, metronidazole, and tetracycline. The majority of affected patients had preexisting chronic periodontitis. All abscesses clinically demonstrated bleeding on probing and suppuration. The periodontal abscess was most commonly associated with lower incisors and canines, followed by lower molars and then upper incisor and canine teeth. Fusobacterium spp. (73%) was the most frequently detected species followed by Prevotella intermedia/nigrescens (65%), Porphyromonas gingivalis (46%) and Aggregatibacter actinomycetemcomitans (24%). The detected clinical isolates of certain bacteria demonstrated resistance to all tested antibiotics except azithromycin. We conclude that Fusobacterium spp., P. intermedia/nigrescens, P. gingivalis, C. rectus, T. forsythia and A. actinomycetemcomitans are closely associated with periodontal abscess. Bacterial species associated with periodontal abscess demonstrated some level of antimicrobial resistance to amoxicillin, metronidazole and tetracycline while antibiotic resistance to azithromycin could not be demonstrated.
Active Control of Low-Frequency Noise through a Single Top-Hung Window in a Full-Sized Room
Authors: Lam Bhan , Shi Dongyuan , Belyi Valiantsin , Wen Shulin , Gan Woon-Seng , Li Kelvin , Lee Irene
Published: September 2020

The push for greater urban sustainability has increased the urgency of the search for noise mitigation solutions that allow for natural ventilation into buildings. Although a viable active noise control (ANC) solution with up to 10 dB of global attenuation between 100 Hz and 1000 Hz was previously developed for an open window, it had limited low-frequency performance below 300 Hz, owing to the small loudspeakers used. To improve the low-frequency attenuation, four passive radiator-based speakers were affixed around the opening of a top-hung ventilation window. The active control performance between 100 Hz and 700 Hz on a single top-hung window in a full-sized mock-up apartment room was examined. Active attenuation came close to the performance of the passive insulation provided by fully closing the window for expressway traffic and motorbike passing noise types. For a jet aircraft flyby, the performance of active attenuation with the window fully opened was similar to that of passive insulation with fully closed windows. In the case of low-frequency compressor noise, active attenuation’s performance was significantly better than the passive insulation. Overall, between 8 dB and 12 dB of active attenuation was achieved directly in front of the window opening, and up to 10.5 dB of attenuation was achieved across the entire room.
Analysis of the Phenomena Causing Weave and Wobble in Two-Wheelers
Authors: Passigato Francesco , Eisele Andreas , Wisselmann Dirk , Gordner Achim , Diermeyer Frank
Published: September 2020

The present work follows in the tracks of previous studies investigating the stability of motorcycles. Two principal oscillation modes of motorcycles are the well-known wobble and weave modes. The research in this field started about fifty years ago and showed how different motorcycle parameters influence the stability of the mentioned modes. However, there is sometimes a minor lack in the physical analysis of why a certain parameter influences the stability. The derived knowledge can be complemented by some mechanical momentum correlations. This work aims to ascertain, in depth, the physical phenomena that stand behind the influence of fork bending compliance on the wobble mode and behind the velocity dependence of the weave damping behaviour. After a summary of the relevant work in this field, this paper presents different rigid body simulation models with increasing complexity and discusses the related eigenvalue analysis and time behaviour. With these models, the mentioned modes are explained and the physical phenomena only partly covered by the literature are shown. Finally, the influence of the rider model on weave and wobble is presented.
Energy-Based Prognosis of the Remaining Useful Life of the Coating Segments in Hot Rolling Mill
Authors: Anagiannis Ioannis , Nikolakis Nikolaos , Alexopoulos Kosmas
Published: September 2020

The field of prognostic maintenance aims at predicting the remaining time for a system or component to continue being used under the desired performance. This time is usually named as Remaining Useful Life (RUL). The current study proposes a novel approach for the RUL estimation of coating segments placed on a hot rolling mill machine. A prediction method was developed, providing real-time updates of the RUL prediction during the rolling milling process. The proposed approach performs energy analysis on measurements of segment surface temperatures and hydraulic forces. It uses nonparametric statistical processes to update the predictions, within a prediction horizon/window, indicating the number of remaining products to be processed. To assess the probability of failure within the defined prediction window, Maximum Likelihood Estimation is used. The proposed methodology was implemented in a software prototype in the MATLAB environment and tested in an industrial use case coming from a steel parts manufacturer, facilitating testing and validation of the suggested approach. Real-world data were acquired from the operational machine, while the validation results support that the proposed methodology demonstrates reasonable performance and robustness against product type variations.
Extending Drag-and-Drop Actions-Based Model-to-Model Transformations with Natural Language Processing
Authors: Danenas Paulius , Skersys Tomas , Butleris Rimantas
Published: September 2020

Model-to-model (M2M) transformations are among the key components of model-driven development, enabling a certain level of automation in the process of developing models. The developed solution of using drag-and-drop actions-based M2M transformations contributes to this purpose by providing a flexible, reusable, customizable, and relatively easy-to-use transformation method and tool support. The solution uses model-based transformation specifications triggered by user-initiated drag-and-drop actions within the model deployed in a computer-aided software engineering (CASE) tool environment. The transformations are called partial M2M transformations, meaning that a specific user-defined fragment of the source model is being transformed into a specific fragment of the target model and not running the whole model-level transformation. In this paper, in particular, we present the main aspects of the developed extension to that M2M transformation method, delivering a set of natural language processing (NLP) techniques on both the conceptual and implementation level. The paper addresses relevant developments and topics in the field of natural language processing and presents a set of operators that can be used to satisfy the needs of advanced textual preprocessing in the scope of M2M transformations. Also in this paper, we describe the extensions to the previous M2M transformation metamodel necessary for enabling the solution’s NLP-related capabilities. The usability and actual benefits of the proposed extension are introduced by presenting a set of specific partial M2M transformation use cases where natural language processing provides actual solutions to previously unsolvable situations when using the previous M2M transformation development.
A Comparative Evaluation of Nature Inspired Algorithms for Telecommunication Network Design
Authors: Kozdrowski Stanisław , Żotkiewicz Mateusz , Wnuk Kacper , Sikorski Arkadiusz , Sujecki Sławomir
Published: September 2020

The subject of the study was an application of nature-inspired metaheuristic algorithms to node configuration optimization in optical networks. The main objective of the optimization was to minimize capital expenditure, which includes the costs of optical node resources, such as transponders and amplifiers used in a new generation of optical networks. For this purpose a model that takes into account the physical phenomena in the optical network is proposed. Selected nature-inspired metaheuristic algorithms were implemented and compared with a reference, deterministic algorithm, based on linear integer programming. For the cases studied the obtained results show that there is a large advantage in using metaheuristic algorithms. In particular, the evolutionary algorithm, the bees algorithm and the harmony search algorithm showed superior performance for the considered data-sets corresponding to large optical networks; the integer programming-based algorithm failed to find an acceptable sub-optimal solution within the assumed maximum computational time. All optimization methods were compared for selected instances of realistic teletransmission networks of different dimensions subject to traffic demand sets extracted from real traffic data.
An Experimentally Validated Numerical Model for the Near-Field Explosion of an Ammunition Storage Magazine
Authors: Hung Cheng-Wei , Tsai Ying-Kuan , Chen Tai-An , Wu Pin-Wen
Published: September 2020

The complexity of the explosions makes it difficult to evaluate a munition storage site’s safety. The peak overpressure associated with a blast wave that propagates from a blast is the governing factor that determines the damage to the buildings around the area. Current codes for predicting the blast pressure from an explosion are mostly applicable for a relatively long-range explosion instead of a near-field explosion. This study evaluated the rationale for the current criteria to assess limitations in the different methods and propose an alternative approach based on experimental and numerical results. This study used a small number of explosives and a small-sized ammunition storage magazine specimen to conduct explosion experiments inside an ammunition storage magazine. The ratio of the blast pressure outside the storage magazine to that at the portal of the storage magazine was compared with the empirical equations and experiments from the references, which were more conservative than the experimental values. The optimal exponential equation was proposed after a regression analysis; this equation is applicable to 1 to 653 times the portal diameter outside the ammunition storage magazine. In terms of the effect of a retaining wall on the blast inside the storage magazine, the longitudinal’s extreme value was reduced by 37–42%, while that of the transverse blast was increased by 8–20%. In terms of the numerical simulations, the extreme value of the external blast within one to five times the portal diameter range outside the ammunition storage magazine could be predicted effectively.
A Hierarchical Coordinative Control Strategy for Solid State Transformer Based DC Microgrids
Authors: Li Zheng , Zheng Tao , Wang Yani , Yang Chang
Published: September 2020

A solid state transformer (SST), as a kind of energy router in the Energy Internet, provides a unified access point for AC or DC distributed power subjects. However, the DC-link capacitors inside the SST will suffer huge voltage fluctuations when the output power of the microgrid changes dramatically. With respect to this problem, caused by the random and intermittent characteristics of distributed generation (DG), a hierarchical coordinative control strategy is proposed. Compared with the common independent control, the proposed method not only makes full use of the regulation capacity of super capacitors, but also enhances the dynamic power tracking speed and reduces the speed difference between different stages of an SST. The dynamic voltage response under the proposed method is analyzed in frequency domain and compared with the independent control. To validate the effectiveness of the coordinative control strategy, a simulation model of an SST-based grid-connected DC microgrid system is established, and the topology of the SST is improved. The voltage stability of the DC bus is compared under different control strategies, and the coordinative control strategy is also verified, effectively under transition conditions.
Detection of Airborne Biological Particles in Indoor Air Using a Real-Time Advanced Morphological Parameter UV-LIF Spectrometer and Gradient Boosting Ensemble Decision Tree Classifiers
Authors: Crawford Ian , Topping David , Gallagher Martin , Forde Elizabeth , Lloyd Jonathan R. , Foot Virginia , Stopford Chris , Kaye Paul
Published: September 2020

We present results from a study evaluating the utility of supervised machine learning to classify single particle ultraviolet laser-induced fluorescence (UV-LIF) signatures to investigate airborne primary biological aerosol particle (PBAP) concentrations in a busy, multifunctional building using a Multiparameter Bioaerosol Spectrometer. First we introduce and demonstrate a gradient boosting ensemble decision tree algorithm’s ability to accurately classify laboratory generated PBAP samples into broad taxonomic classes with a high level of accuracy. We then develop a framework to appraise the classification accuracy and performance using the Hellinger distance metric to compare product parameter probability density function similarity; this framework showed that key training classes were sufficiently different in terms of particle fluorescence and morphology to facilitate classification. We also demonstrate the utility of including advanced morphological parameters to minimise inter-class conflation and improve classification confidence, where relying on the fluorescent spectra alone would likely result in misattribution. Finally, we apply these methods to ambient data collected within a large multi-functional building where ambient bacterial- and fungal-like classes were identified to display trends corresponding to human activity; fungal-like classes displayed a consistent diurnal trend with a maximum at midday and hourly peaks correlating to movements within the building; bacteria-like aerosol displayed complex, episodic events during opening hours. All PBAP classes fell to low baseline concentrations when the building was unoccupied overnight and at weekends.
The c.*52 A/G and c.*773 A/G Genetic Variants in the UTR′3 of the LDLR Gene Are Associated with the Risk of Acute Coronary Syndrome and Lower Plasma HDL-Cholesterol Concentration
Authors: Vargas-Alarcon Gilberto , Perez-Mendez Oscar , Ramirez-Bello Julian , Posadas-Sanchez Rosalinda , Gonzalez-Pacheco Hector , Escobedo Galileo , Nieto-Lima Betzabe , Carreon-Torres Elizabeth , Fragoso Jose Manuel
Published: September 2020

Dyslipidemia has a substantial role in the development of acute coronary syndrome (ACS). Low-density lipoprotein receptor (LDLR) plays a critical role in plasma lipoprotein hemostasis, which is involved in the formation of atherosclerotic plaque. This study aimed to evaluate whether LDLR gene polymorphisms are significantly associated with ACS and the plasma lipids profile. Three LDLR gene polymorphisms located in the UTR′3 region (c.*52 A/G, c.*504 A/G, and c.* 773 A/G) were determined using TaqMan genotyping assays in a group of 618 ACS patients and 666 healthy controls. Plasma lipids profile concentrations were determined by enzymatic/colorimetric assays. Under co-dominant and recessive models, the c.*52 A allele of the c.*52 A/G polymorphism was associated with a higher risk of ACS (OR = 2.02, pCCo-dom = 0.033, and OR = 2.00, pCRes = 0.009, respectively). In the same way, under co-dominant and recessive models, the c.*773 G allele of the c.*773 A/G polymorphism was associated with a high risk of ACS (OR = 2.04, pCCo-dom = 0.027, and OR = 2.01, pCRes = 0.007, respectively). The “AAG” haplotype was associated with a high risk of ACS (OR = 1.22, pC = 0.016). The c.*52 AA genotype showed a lower HDL-C concentration than individuals with the GG genotype. In addition, carriers of c.*773 GG genotype carriers had a lower concentration of the high-density lipoprotein-cholesterol (HDL-C) than subjects with the AA genotype. Our data suggest the association of the LDLRc.*773 A/G and LDLR c.*52 A/G polymorphisms with both the risk of developing ACS and with a lower concentration of HDL-C in the study population.
Anti-BCMA Immunotoxins: Design, Production, and Preclinical Evaluation
Authors: Bera Tapan K.
Published: September 2020

Multiple myeloma (MM) is a B-cell malignancy that is incurable for a majority of patients. B-cell maturation antigen (BCMA) is a lineage-restricted differentiation protein highly expressed in multiple myeloma cells but not in other normal tissues except normal plasma B cells. Due to the restricted expression and being a cell surface membrane protein, BCMA is an ideal target for immunotherapy approaches in MM. Recombinant immunotoxins (RITs) are a novel class of protein therapeutics that are composed of the Fv or Fab portion of an antibody fused to a cytotoxic agent. RITs were produced by expressing plasmids encoding the components of the anti-BCMA RITs in E. coli followed by inclusion body preparation, solubilization, renaturation, and purification by column chromatography. The cytotoxic activity of RITs was tested in vitro by WST-8 assays using BCMA expressing cell lines and on cells isolated from MM patients. The in vivo efficacy of RITs was tested in a xenograft mouse model using BCMA expressing multiple myeloma cell lines. Anti-BCMA recombinant immunotoxins are very effective in killing myeloma cell lines and cells isolated from myeloma patients expressing BCMA. Two mouse models of myeloma showed that the anti-BCMA immunotoxins can produce a long-term complete response and warrant further preclinical development.
Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)
Authors: Yan Yan , Chang Clare , Su Junyi , Venø Morten T. , Kjems Jørgen
Published: September 2020

Multipotent stem cells (MSCs) are used in various therapeutic applications based on their paracrine secretion activity. Here, we set out to identify and characterize the paracrine factors released during osteoblastogenesis, with a special focus on small non-coding RNAs released in extracellular vesicles (EVs). Bone marrow stem cells (BMSCs) and adipose stem cells (ASCs) from healthy human donors were used as representatives of MSCs. We isolated EVs secreted before and after induction of osteoblastic differentiation and found that the EVs contained a specific subset of microRNAs (miRNAs) and tRNA-derived small RNAs (tsRNA) compared to their parental cells. Osteoblastic differentiation had a larger effect on the small RNA profile of BMSC-EVs relative to ASC-EVs. Our data showed that EVs from different MSC origin exhibited distinct expression profiles of small RNA profiles when undergoing osteoblastogenesis, a factor that should be taken into consideration for stem cell therapy.
Granzyme B Degraded Type IV Collagen Products in Serum Identify Melanoma Patients Responding to Immune Checkpoint Blockade
Authors: Jensen Christina , Sinkeviciute Dovile , Madsen Daniel Hargbøl , Önnerfjord Patrik , Hansen Morten , Schmidt Henrik , Karsdal Morten Asser , Svane Inge Marie , Willumsen Nicholas
Published: September 2020

A T-cell permissive tumor microenvironment, characterized by the presence of activated T cells and low fibrotic activity is crucial for response to immune checkpoint inhibitors (ICIs). Granzyme B has been shown to promote T-cell migration through the basement membrane by the degradation of type IV collagen. In this study, we evaluated the biomarker potential of measuring granzyme B-mediated degradation of type IV collagen (C4G) in combination with a fibroblast activation biomarker (PRO-C3) non-invasively for identifying metastatic melanoma patients responding to the ICI ipilimumab. A monoclonal antibody was generated against C4G and used to develop a competitive electro-chemiluminescence immunoassay. C4G and PRO-C3 were measured in pretreatment serum from metastatic melanoma patients (n = 54). The C4G assay was found specific for a granzyme B-generated neo-epitope on type IV collagen. The objective response rate (ORR) was 2.6-fold higher (18% vs. 7%) in patients with high C4G levels (>25th percentile) vs. low levels (≤25th percentile). Likewise, high C4G levels at baseline were associated with longer overall survival (OS) (log-rank, p = 0.040, and hazard ratio (HR) = 0.48, 95%CI: 0.24–0.98, p = 0.045). Combining high C4G with low PRO-C3 correlated with improved OS with a median OS of 796 days vs. 273 days (p = 0.0003) and an HR of 0.30 (95%CI: 0.15–0.60, p = 0.0006). In conclusion, these results suggest that high granzyme B degraded type IV collagen (C4G) combined with low PRO-C3 quantified non-invasively has the potential to identify the responders to ICI therapy.
Novel Model to Predict HCC Recurrence after Liver Transplantation Obtained Using Deep Learning: A Multicenter Study
Authors: Nam Joon Yeul , Lee Jeong-Hoon , Bae Junho , Chang Young , Cho Yuri , Sinn Dong Hyun , Kim Bo Hyun , Kim Seoung Hoon , Yi Nam-Joon , Lee Kwang-Woong , Kim Jong Man , Park Joong-Won , Kim Yoon Jun , Yoon Jung-Hwan , Joh Jae-Won , Suh Kyung-Suk
Published: September 2020

Several models have been developed using conventional regression approaches to extend the criteria for liver transplantation (LT) in hepatocellular carcinoma (HCC) beyond the Milan criteria. We aimed to develop a novel model to predict tumor recurrence after LT by adopting artificial intelligence (MoRAL-AI). This study included 563 patients who underwent LT for HCC at three large LT centers in Korea. Derivation (n = 349) and validation (n = 214) cohorts were independently established. The primary outcome was time-to-recurrence after LT. A MoRAL-AI was derived from the derivation cohort with a residual block-based deep neural network. The median follow-up duration was 74.7 months (interquartile-range, 18.5–107.4); 204 patients (36.2%) had HCC beyond the Milan criteria. The optimal model consisted of seven layers including two residual blocks. In the validation cohort, the MoRAL-AI showed significantly better discrimination function (c-index = 0.75) than the Milan (c-index = 0.64), MoRAL (c-index = 0.69), University of California San Francisco (c-index = 0.62), up-to-seven (c-index = 0.50), and Kyoto (c-index = 0.50) criteria (all p < 0.001). The largest weighted parameter in the MoRAL-AI was tumor diameter, followed by alpha-fetoprotein, age, and protein induced by vitamin K absence-II. The MoRAL-AI had better predictability of tumor recurrence after LT than conventional models. The MoRAL-AI can also evolve with further data.
Blocking Y-Box Binding Protein-1 through Simultaneous Targeting of PI3K and MAPK in Triple Negative Breast Cancers
Authors: Tiwari Aadhya , Iida Mari , Kosnopfel Corinna , Abbariki Mahyar , Menegakis Apostolos , Fehrenbacher Birgit , Maier Julia , Schaller Martin , Brucker Sara Y. , Wheeler Deric L. , Harari Paul M. , Rothbauer Ulrich , Schittek Birgit , Zips Daniel , Toulany Mahmoud
Published: September 2020

The multifunctional protein Y-box binding protein-1 (YB-1) regulates all the so far described cancer hallmarks including cell proliferation and survival. The MAPK/ERK and PI3K/Akt pathways are also the major pathways involved in cell growth, proliferation, and survival, and are the frequently hyperactivated pathways in human cancers. A gain of function mutation in KRAS mainly leads to the constitutive activation of the MAPK pathway, while the activation of the PI3K/Akt pathway occurs either through the loss of PTEN or a gain of function mutation of the catalytic subunit alpha of PI3K (PIK3CA). In this study, we investigated the underlying signaling pathway involved in YB-1 phosphorylation at serine 102 (S102) in KRAS(G13D)-mutated triple-negative breast cancer (TNBC) MDA-MB-231 cells versus PIK3CA(H1047R)/PTEN(E307K) mutated TNBC MDA-MB-453 cells. Our data demonstrate that S102 phosphorylation of YB-1 in KRAS-mutated cells is mainly dependent on the MAPK/ERK pathway, while in PIK3CA/PTEN-mutated cells, YB-1 S102 phosphorylation is entirely dependent on the PI3K/Akt pathway. Independent of the individual dominant pathway regulating YB-1 phosphorylation, dual targeting of MEK and PI3K efficiently inhibited YB-1 phosphorylation and blocked cell proliferation. This represents functional crosstalk between the two pathways. Our data obtained from the experiments, applying pharmacological inhibitors and genetic approaches, shows that YB-1 is a key player in cell proliferation, clonogenic activity, and tumor growth of TNBC cells through the MAPK and PI3K pathways. Therefore, dual inhibition of these two pathways or single targeting of YB-1 may be an effective strategy to treat TNBC.
When the Search for Stemness Genes Meets the Skin Substitute Bioengineering Field: KLF4 Transcription Factor under the Light
Authors: Fortunel Nicolas O. , Martin Michèle T.
Published: September 2020

The transcription factor “Kruppel-like factor 4” (KLF4) is a central player in the field of pluripotent stem cell biology. In particular, it was put under the spotlight as one of the four factors of the cocktail originally described for reprogramming into induced pluripotent stem cells (iPSCs). In contrast, its possible functions in native tissue stem cells remain largely unexplored. We recently published that KLF4 is a regulator of “stemness” in human keratinocytes. We show that reducing the level of expression of this transcription factor by RNA interference or pharmacological repression promotes the ex vivo amplification and regenerative capacity of two types of cells of interest for cutaneous cell therapy: native keratinocyte stem and progenitor cells from adult epidermis, which have been used for more than three decades in skin graft bioengineering, and keratinocytes generated by the lineage-oriented differentiation of embryonic stem cells (ESCs), which have potential for the development of skin bio-bandages. At the mechanistic level, KLF4 repression alters the expression of a large set of genes involved in TGF-β1 and WNT signaling pathways. Major regulators of TGF-β bioavailability and different TGF-β receptors were targeted, notably modulating the ALK1/Smad1/5/9 axis. At a functional level, KLF4 repression produced an antagonist effect on TGF-β1-induced keratinocyte differentiation.
Neurodegeneration Caused by S1P-Lyase Deficiency Involves Calcium-Dependent Tau Pathology and Abnormal Histone Acetylation
Authors: Alam Shah , Piazzesi Antonia , Abd El Fatah Mariam , Raucamp Maren , van Echten-Deckert Gerhild
Published: September 2020

We have shown that sphingosine 1-phosphate (S1P) generated by sphingosine kinase 2 (SK2) is toxic in neurons lacking S1P-lyase (SGPL1), the enzyme that catalyzes its irreversible cleavage. Interestingly, patients harboring mutations in the gene encoding this enzyme (SGPL1) often present with neurological pathologies. Studies in a mouse model with a developmental neural-specific ablation of SGPL1 (SGPL1fl/fl/Nes) confirmed the importance of S1P metabolism for the presynaptic architecture and neuronal autophagy, known to be essential for brain health. We now investigated in SGPL1-deficient murine brains two other factors involved in neurodegenerative processes, namely tau phosphorylation and histone acetylation. In hippocampal and cortical slices SGPL1 deficiency and hence S1P accumulation are accompanied by hyperphosphorylation of tau and an elevated acetylation of histone3 (H3) and histone4 (H4). Calcium chelation with BAPTA-AM rescued both tau hyperphosphorylation and histone acetylation, designating calcium as an essential mediator of these (patho)physiological functions of S1P in the brain. Studies in primary cultured neurons and astrocytes derived from SGPL1fl/fl/Nes mice revealed hyperphosphorylated tau only in SGPL1-deficient neurons and increased histone acetylation only in SGPL1-deficient astrocytes. Both could be reversed to control values with BAPTA-AM, indicating the close interdependence of S1P metabolism, calcium homeostasis, and brain health.