bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Solvent Vapor Atmosphere on Photovoltaic Performance of Perovskite Solar Cells
Authors: He Miao , Chen Shuyan , Wang Taoran , Xu Gu , Liu Na , Xu Fan
Published: March 2023

Tremendous efforts have been devoted to facilitating the commercialization of perovskite solar cells (PSCs) in the past decade. However, the influence of solvent vapor atmosphere on PSC device performance during its fabrication still lacks related investigations. Here, by using three commonly employed solvent vapors during the perovskite annealing process, i.e., isopropanol, chlorobenzene and dimethylformamide, we reveal the effects of atmosphere on related perovskite film properties and device performance. The results indicate that perovskite films prepared under these external solvent vapors exhibit distinct crystalline phases, morphologies and optical properties from films under normal conditions (nitrogen gas), resulting in a significant drop in power conversion efficiency from the initial 20.01% to the lowest of only ~15%. Our work highlights the importance of atmospheric effects in preparing efficient PSCs for scalable fabrication and commercialization.
Citrate-Assisted One-Pot Hydrothermal Preparation of Carbonated Hydroxyapatite Microspheres
Authors: Qi Mei-li , Wu Yanling , Sun Cuicui , Zhang Haijun , Yao Shengkun
Published: March 2023

Carbonated hydroxyapatite (CHA) microspheres have aroused wide concern in biofields because of their excellent biological and surface properties. However, the facile preparation of CHA microspheres from organic compounds, especially the microstructural transformation during synthesis, has been rarely reported. In this work, CHA microspheres with an average diameter of 2.528 μm and a BET surface area of 51.0658 m2/g were synthesized via a one-pot hydrothermal method at 180 °C for 10 h by using calcium chloride, diammonium hydrogen phosphate, urea, and trisodium citrate (TSC) with a molar ratio of TSC to Ca of 1:2. The effects of hydrothermal treatment temperature and molar ratio of TSC to Ca on the morphology of the products were investigated. As a chelating agent, TSC is crucial to the formation of CHA microspheres during the hydrothermal homogeneous precipitation process. A possible mechanism of the microstructural transformation from bundle to dumbbell, dumbbell ball, and finally, microspheres regulated by TSC and urea was proposed. The CHA microspheres can be used as effective drug carriers for biomedical applications.
Parasites in Imported Edible Fish and a Systematic Review of the Pathophysiology of Infection and the Potential Threat to Australian Native Aquatic Species
Authors: Williams Michelle , Hernandez-Jover Marta , Shamsi Shokoofeh
Published: March 2023

Previous research has shown that certain types of edible fish imported into Australia are infected with Euclinostomum/Isoparorchis digenetic trematodes. In the present study, imported Channa fish were examined for parasites which were then morphologically identified to the lowest taxonomic unit possible. Here we provide the first Australian report of Pallisentis sp. Van Cleave, 1928 (Prevalence (P) 35.9%) of family Quadrigyridae; Genarchopsis sp. Ozaki, 1925 (P. 16.5%), family Derogenidae and Senga sp. Dollfus, 1934 (P. 4.8%) in edible imported Channa fish (n = 103). Pallisentis sp. and Senga sp. have invasive hold fast organs which cause significant mechanical damage to fish intestinal structures and Euclinostomum/Isoparorchis cause severe pathology and loss of marketability in infected fish. These exotic parasites, if introduced into Australia, have the potential to negatively impact the health, fecundity, resilience and marketability of native and commercial fish species. Biosecurity is a constant ontogenesis of novel hypothesis based on current scientific discoveries. To further increase understanding of how parasitism impacts fish health, a systematic literature review was conducted and the pathophysiology of infection described. Potential exposure pathways and parasite host associations in Australia are discussed.
Heterozygous Variants in FREM2 Are Associated with Mesiodens, Supernumerary Teeth, Oral Exostoses, and Odontomas
Authors: Kantaputra Piranit Nik , Tripuwabhrut Kanich , Anthonappa Robert P. , Chintakanon Kanoknart , Ngamphiw Chumpol , Adisornkanj Ploy , Porntrakulseree Nop , Olsen Bjorn , Intachai Worrachet , Hennekam Raoul C. , Vieira Alexandre R. , Tongsima Sissades
Published: March 2023

Background: Supernumerary teeth refer to extra teeth that exceed the usual number of dentitions. A mesiodens is a particular form of supernumerary tooth, which is located in the premaxilla region. The objective of the study was to investigate the genetic etiology of extra tooth phenotypes, including mesiodens and isolated supernumerary teeth. Methods: Oral and radiographic examinations and whole-exome sequencing were performed on every patient in our cohort of 122 patients, including 27 patients with isolated supernumerary teeth and 94 patients with mesiodens. A patient who had multiple supernumerary teeth also had odontomas. Results: We identified a novel (c.8498A>G; p.Asn2833Ser) and six recurrent (c.1603C>T; p.Arg535Cys, c.5852G>A; p.Arg1951His, c.6949A>T; p.Thr2317Ser; c.1549G>A; p.Val517Met, c.1921A>G; p.Thr641Ala, and c.850G>C; p.Val284Leu) heterozygous missense variants in FREM2 in eight patients with extra tooth phenotypes. Conclusions: Biallelic variants in FREM2 are implicated in autosomal recessive Fraser syndrome with or without dental anomalies. Here, we report for the first time that heterozygous carriers of FREM2 variants have phenotypes including oral exostoses, mesiodens, and isolated supernumerary teeth.
Whole-Body Magnetic Resonance Tomography and Whole-Body Computed Tomography in Pediatric Polytrauma Diagnostics—A Retrospective Long-Term Two-Center Study
Authors: Raimann Marnie , Ludwig Johanna , Heumann Peter , Rechenberg Ulrike , Goelz Leonie , Mutze Sven , Schellerer Vera , Ekkernkamp Axel , Bakir Mustafa Sinan
Published: March 2023

Although serious accidents remain the leading cause of pediatric mortality, protocols to orient diagnostic procedures towards a certain type of initial imaging are widely needed. Since 2007, we have performed whole-body magnetic resonance imaging (WBMR) and whole-body computed tomography (WBCT) for diagnoses of severely injured children. We retrospectively reviewed 134 WBMR and 158 WBCT in patients younger than 16 years that were performed at two trauma centers between 2007 and 2018. A higher Injury Severity Score (ISS) was found in WBCT vs. WBMR (10.6 vs. 5.8; p = 0.001), but without any significant difference in mortality. The WBMR was significantly preferred at younger ages (9.6 vs. 12.8 years; p < 0.001). The time between patient’s arrival until diagnosis was 2.5 times longer for WBCT (92.1 vs. 37.1 min; p < 0.001). More patients in the CT group received analgesic sedation and/or intubation at 37.3% vs. 21.6% in the MRI group. Of these patients, 86.4% (CT) and 27.6% (MRI) were already preclinically sedated (p < 0.001). Correspondingly, 72.4% of the patients were first sedated in-hospital for MRIs. In conclusion, WBMR is an alternative and radiation-free imaging method for high-energy-traumatized children. Although the selected diagnostics seemed appropriate, limitations regarding longer duration or additional analgesic sedation are present, and further studies are needed.
Aquatic Macroinvertebrates as Bioindicators of Water Quality: A Study of an Ecosystem Regulation Service in a Tropical River
Authors: Orozco-González Christopher E. , Ocasio-Torres María E.
Published: March 2023

In this study, aquatic macroinvertebrates were used as bioindicators to determine the ecological conditions of the Lapa River, located between the municipalities of Cayey and Salinas, Puerto Rico. The water quality of the river was evaluated through the calculation of the Puerto Rico Biological Monitoring Working Party (BMWP-PR), as well as its degree of contamination using the Puerto Rico Family Biotic Index (FBI-PR). Bimonthly sampling was conducted across four sampling sites for a period of 12 months. The sampling sites were positioned upstream and downstream within the nature reserve, and outside and downstream its borders. The BMWP-PR results showed that the site upstream-inside the nature reserve had good water quality, and the site downstream-inside the nature reserve had regular water quality, showing some areas with eutrophication. The water quality outside-downstream from the nature reserve was poor. The FBI-PR results showed that there was mild organic contamination inside the nature reserve, while there was substantial organic contamination in the site that was outside-downstream from the nature reserve. We concluded that the section of the river located within the nature reserve had better ecological conditions than the stretch of the river located outside-downstream of the nature reserve, because it is located within a protected area that has barely been impacted by human activity.
An Introduction to the Special Issue on Digital and Collaborative Higher Education: The Case of the Erasmus+ OpenU Project
Authors: Pina Stranger Alvaro , Varas German , Mobuchon Gaëlle
Published: March 2023

This Special Issue casts a scholarly lens on collaboration among higher education institutions (HEIs), promoted by digital transformation and “digitalization” [...]
Electrocatalytic Reduction of O2 by ITO-IrOx: Implication for Dissolved Oxygen Sensor in the Alkaline Medium
Authors: Siddika Munira , Hasan Md. Mahmudul , Oyshi Tahamida A. , Hasnat Mohammad A.
Published: March 2023

Water pollution has badly affected human health, aquatic life, and the ecosystem. The purity of surface water can be measured in terms of dissolved oxygen (DO) measurements. Hence, it is desirable to have a portable and simple-to-use dissolved oxygen sensor. One possible remedy is an electrochemical sensor. Thus, we proposed an ITO-IrOx electrocatalyst for an effective and interference-free DO sensor utilizing the principle of oxygen reduction reaction (ORR). The ITO-IrOx was characterized using cyclic voltammetry (CV), scanning electron microscopy (SEM), electrochemical impedance spectrometry (EIS), X-ray photoelectron spectroscopy (XPS), and reflectance spectroscopy-based techniques. Reflectance spectra of the ITO-IrOx electrode showed the photoresist capability. The EIS spectra revealed lower charge transfer resistance for the ITO-IrOx electrode in ORR. The IrOx film on ITO exhibited a quick (one electron, α = 1.00), and reversible electron transfer mechanism. The electrode demonstrated high stability for oxygen sensing, having a limit of detection (LOD) of 0.49 ppm and interference-free from some common ions (nitrate, sulphate, chloride etc.) found in water.
Fog Computing Resource-Scheduling Strategy in IoT Based on Artificial Bee Colony Algorithm
Authors: Liu Weimin , Li Chen , Zheng Aiyun , Zheng Zhi , Zhang Zhen , Xiao Yao
Published: March 2023

As the amount of data input increases, fog devices on IoT edge networks become increasingly inefficient. However, a well-designed fog computing resource-scheduling strategy can help to reduce excessive time delays and energy consumption. Therefore, in this paper, we propose an efficient fog computing resource-scheduling strategy. First, we used particle swarm optimization (PSO) to determine the optimal load balance among fog nodes and to obtain the optimal computation time and energy consumption in a single fog cluster. Second, we designed a particle swarm genetic joint optimization artificial bee colony algorithm (PGABC) to optimize the task scheduling among fog clusters based on the time and energy consumption obtained from load balancing. In addition, PGABC was used to optimize the task-scheduling model, which further reduced the delay and energy consumption of fog computing. The experimental results show that the time delay that was calculated using the proposed PGABC algorithm in the given model was reduced by 1.04%, 15.9%, and 28.5%, compared to GABC, ABC, and PSO, respectively, and the energy consumption was reduced by 3.9%, 6.6%, and 12.6%, respectively. The proposed resource-scheduling strategy reduced the delay by approximately 31.25%, 27.8%, 27.8%, and 25.4%, and the energy consumption by approximately 9.7%, 33.3%, 32%, and 29.6%, compared to SJF–PSO, PGABC-R, HSF.ABC&PSO, and MFO, respectively.
Research on Intelligent Disinfection-Vehicle System Design and Its Global Path Planning
Authors: Chen Lifang , Yang Huogen , Chen Zhichao , Feng Zhicheng
Published: March 2023

We aimed to research the design and path-planning methods of an intelligent disinfection-vehicle system. A ROS (robot operating system) system was utilized as the control platform, and SLAM (simultaneous localization and mapping) technology was used to establish an indoor scene map. On this basis, a new path-planning method combining the A* algorithm and the Floyd algorithm is proposed to ensure the safety, efficiency, and stability of the path. Simulation results show that with the average shortest distance between obstacles and paths of 0.463, this algorithm reduces the average numbers of redundant nodes and turns in the path by 70.43% and 31.1%, respectively, compared to the traditional A* algorithm. The algorithm has superior performance in terms of safety distance, path length, and redundant nodes and turns. Additionally, a mask recognition and pedestrian detection algorithm is utilized to ensure public safety. The results of the study indicate that the method has satisfactory performance. The intelligent disinfection-vehicle system operates stably, meets the indoor mapping requirements, and can recognize pedestrians and masks.
Comprehensive Review Based on the Impact of Integrating Electric Vehicle and Renewable Energy Sources to the Grid
Authors: Sinha Pampa , Paul Kaushik , Deb Sanchari , Sachan Sulabh
Published: March 2023

Global warming, pollution, and the depletion of fossil fuels have compelled human beings to explore alternate sources of energy and cleaner modes of transport. In recent years, renewable energy sources (RES) have been massively introduced to the grid. Furthermore, Electric Vehicles (EVs) are becoming popular as a cleaner mode of transport. However, the introduction of RESs and EVs to the grid has imposed additional challenges on the grid operators because of their random nature. This review aims to focus on the integration of RES and EVs to the grid, thereby presenting the global status of RESs and EVs, the impact of integrating RESs and EVs to the grid, the challenges of integrating RES and EV to the grid, optimization techniques for EV and RES integration to the grid, and mitigation techniques. A total of 153 research papers are meticulously reviewed, and the findings are put forward in this review. Thus, this review will put forward the latest developments in the area of EV and RES integration into the grid and will enlighten the researchers with the unsolved questions in the area that need investigation.
Comprehensive Review of Recent Advancements in Battery Technology, Propulsion, Power Interfaces, and Vehicle Network Systems for Intelligent Autonomous and Connected Electric Vehicles
Authors: Abro Ghulam E Mustafa , Zulkifli Saiful Azrin B. M. , Kumar Kundan , El Ouanjli Najib , Asirvadam Vijanth Sagayan , Mossa Mahmoud A.
Published: March 2023

Numerous recent innovations have been achieved with the goal of enhancing electric vehicles and the parts that go into them, particularly in the areas of managing energy, battery design and optimization, and autonomous driving. This promotes a more effective and sustainable eco-system and helps to build the next generation of electric car technology. This study offers insights into the most recent research and advancements in electric vehicles (EVs), as well as new, innovative, and promising technologies based on scientific data and facts associated with e-mobility from a technological standpoint, which may be achievable by 2030. Appropriate modeling and design strategies, including digital twins with connected Internet of Things (IoT), are discussed in this study. Vehicles with autonomous features have the potential to increase safety on roads, increase driving economy, and provide drivers more time to focus on other duties thanks to the Internet of Things idea. The enabling technology that entails a car moving out of a parking spot, traveling along a long highway, and then parking at the destination is also covered in this article. The development of autonomous vehicles depends on the data obtained for deployment in actual road conditions. There are also research gaps and proposals for autonomous, intelligent vehicles. One of the many social concerns that are described is the cause of an accident with an autonomous car. A smart device that can spot strange driving behavior and prevent accidents is briefly discussed. In addition, all EV-related fields are covered, including the likely technical challenges and knowledge gaps in each one, from in-depth battery material sciences through power electronics and powertrain engineering to market assessments and environmental assessments.
An Increase in the Energy Efficiency of a New Design of Pumps for Nuclear Power Plants
Authors: Pavlenko Ivan , Ciszak Olaf , Kondus Vladyslav , Ratushnyi Oleksandr , Ivchenko Oleksandr , Kolisnichenko Eduard , Kulikov Oleksandr , Ivanov Vitalii
Published: March 2023

The reliability of pumping units at nuclear power plants (NPPs) is critical in terms of their energy efficiency and safety. Remarkably, WWER-1000 reactors at Ukrainian NPPs are equipped with outdated pumping units that have already served their full-service life. This fact leads to an urgent need to develop a new, more efficient pump. In the article, a promising pump, ACNA 600-35, was developed. It was designed to increase the energy efficiency of pumps TX 800/70/8-K-2E, applied at the holding pool and the industrial circuit of the nuclear reactor. Since these pumps should be imported from the monopoly suppliers, this affects both the energy efficiency of pumping equipment and the energy independence of Eastern Europe. The proposed pump ACNA 600-35 is characterized by an increased efficiency of up to 0.12–0.13 compared with the TX 800/70/8-K-2E pump. In general, the life cycle cost of the proposed pump is 15–20% lower than for the analog TX 800/70/8-K-2E. The design of the developed pump ACNA 600-35 and the related pumping unit based on its production at industrial facilities allows for further development of the industrial and fuel-energy complex, increasing the state’s energy independence and employment. According to expert estimates, the average economic effect from supplying the developed pump can reach 10 mln USD/year.
The Potential of Green Schoolyards for Healthy Child Development: A Conceptual Framework
Authors: van den Bogerd Nicole , Hovinga Dieuwke , Hiemstra Jelle A. , Maas Jolanda
Published: March 2023

To provide children more opportunities to interact with nature, an increasing number of schools are ‘greening’ their schoolyards by including abiotic and biotic elements such as vegetation, sand, water, logs, and stones. Although the value of these green, nature-rich schoolyards is increasingly acknowledged, research has focused on a narrow set of child development outcomes. This paper presents a conceptual framework that gives insight into the potential short- and long-term benefits of green schoolyards related to children’s physical, cognitive, social-emotional, and moral development, and the pathways through which they may occur. We argue that a green schoolyard can facilitate diverse behaviors and activities, provide sensory and embodied nature experiences, provide a restorative environment, support biodiversity, and provide a resilient environment that supports climate resilience and mitigates environmental nuisance. These five functions of green schoolyards can act as pathways to help foster healthy child development. In doing so, the framework provides guidance for future research. Although more research is needed to validate the conceptual framework, it seems that through the proposed pathways, green schoolyards can be a promising nature-based intervention to promote healthy child development.
Analysis of Spectral Separability for Detecting Burned Areas Using Landsat-8 OLI/TIRS Images under Different Biomes in Brazil and Portugal
Authors: Pacheco Admilson da Penha , da Silva Junior Juarez Antonio , Ruiz-Armenteros Antonio Miguel , Henriques Renato Filipe Faria , de Oliveira Santos Ivaneide
Published: March 2023

Fire is one of the natural agents with the greatest impact on the terrestrial ecosystem and plays an important ecological role in a large part of the terrestrial surface. Remote sensing is an important technique applied in mapping and monitoring changes in forest landscapes affected by fires. This study presents a spectral separability analysis for the detection of burned areas using Landsat-8 OLI/TIRS images in the context of fires that occurred in different biomes of Brazil (dry ecosystem) and Portugal (temperate forest). The research is based on a fusion of spectral indices and automatic classification algorithms scientifically proven to be effective with as little human interaction as possible. The separability index (M) and the Reed–Xiaoli automatic anomaly detection classifier (RXD) allowed the evaluation of the spectral separability and the thematic accuracy of the burned areas for the different spectral indices tested (Burn Area Index (BAI), Normalized Burn Ratio (NBR), Mid-Infrared Burn Index (MIRBI), Normalized Burn Ratio 2 (NBR2), Normalized Burned Index (NBI), and Normalized Burn Ratio Thermal (NBRT)). The analysis parameters were based on spatial dispersion with validation data, commission error (CE), omission error (OE), and the Sørensen–Dice coefficient (DC). The results indicated that the indices based exclusively on the SWIR1 and SWIR2 bands showed a high degree of separability and were more suitable for detecting burned areas, although it was observed that the characteristics of the soil affected the performance of the indices. The classification method based on bitemporal anomalous changes using the RXD anomaly proved to be effective in increasing the burned area in terms of temporal alteration and performing unsupervised detection without relying on the ground truth. On the other hand, the main limitations of RXD were observed in non-abrupt changes, which is very common in fires with low spectral signal, especially in the context of using Landsat-8 images with a 16-day revisit period. The results obtained in this work were able to provide critical information for fire mapping algorithms and for an accurate post-fire spatial estimation in dry ecosystems and temperate forests. The study presents a new comparative approach to classify burned areas in dry ecosystems and temperate forests with the least possible human interference, thus helping investigations when there is little available data on fires in addition to favoring a reduction in fieldwork and gross errors in the classification of burned areas.
Simultaneous Analysis of Bergapten and Schinifoline in Zanthoxylum schinifolium Seeds Using HPLC and UPLC-MS/MS Systems
Authors: Seo Chang-Seob
Published: March 2023

Zanthoxylum schinifolium Siebold et Zuccarini belongs to the Rutaceae family and has been widely used as a spice in East Asian countries such as Korea, China, and Japan. The present study focused on developing and validating a simultaneous analytical method for marker substances (bergapten and schinifoline) in Z. schinifolium seeds. This was achieved using high-performance liquid chromatography with a photo-diode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) systems. In the regression equation, all markers showed a coefficient of determination of ≥0.9990. Marker recovery was 96.90–105.16% (relative standard deviation (RSD) ≤ 2.23), and the intra- and interday precision was RSD < 3.00. Bergapten and schinifoline were detected in the seeds at 1.70–2.85 mg/g and 0.19–0.94 mg/g, respectively. This analytical method will improve quality control of Z. schinifolium seeds. Additionally, this assay will provide basic data and quality assurance for future biological activity experiments or clinical applications.
A Case Series on Pain Accompanying Photoimmunotherapy for Head and Neck Cancer
Authors: Shibutani Yuma , Sato Haruna , Suzuki Shinya , Shinozaki Takeshi , Kamata Hayato , Sugisaki Kazuki , Kawanobe Atushi , Uozumi Shinya , Kawasaki Toshikatsu , Hayashi Ryuichi
Published: March 2023

One of the most severe side effects of photoimmunotherapy (PIT) for head and neck cancer is pain. As there are presently no detailed reports on pain and pain management in PIT, we conducted a retrospective case series study. We conducted a retrospective study of five patients who had received PIT at the National Cancer Center Hospital East between January 2021 and June 2022 using medical chart data. All patients experienced pain, evidenced by an increased numerical rating scale (NRS) after PIT, regardless of the illumination method. The daily change in mean NRS rating shows that the pain was highest on the day of PIT, with ratings of 6.8 and 7.8 for the frontal and cylindrical diffuser methods, respectively; it dropped the following day quickly. Four of the five patients received fentanyl injections for postoperative pain management beginning on postoperative day (POD) 0. All patients who underwent therapy using a cylindrical diffuser required postoperative pain management with opioid drugs. Pain after PIT tended to be most intense immediately after or one hour after illumination and declined the following day, suggesting the need to have a pain relief plan in place in advance.
Mortality Evaluation and Life Expectancy Prediction of Patients with Hepatocellular Carcinoma with Data Mining
Authors: Liu Che-Yu , Cheng Chen-Yang , Yang Szu-Ying , Chai Jyh-Wen , Chen Wei-Hao , Chang Pi-Yi
Published: March 2023

Background: The complexity of systemic variables and comorbidities makes it difficult to determine the best treatment for patients with hepatocellular carcinoma (HCC). It is impossible to perform a multidimensional evaluation of every patient, but the development of guidelines based on analyses of said complexities would be the next best option. Whereas conventional statistics are often inadequate for developing multivariate predictive models, data mining has proven more capable. Patients, methods and findings: Clinical profiles and treatment responses of 537 patients diagnosed with Barcelona Clinic Liver Cancer stages B and C from 2009 to 2019 were retrospectively analyzed using 4 decision tree algorithms. A combination of 19 treatments, 7 biomarkers, and 4 states of hepatitis was tested to determine which combinations would result in survival times greater than a year in duration. Just 2 of the algorithms produced complete models through single trees, which made them only the ones suitable for clinical judgement. A combination of alpha fetoprotein ≤210.5 mcg/L, glutamic oxaloacetic transaminase ≤1.13 µkat/L, and total bilirubin ≤ 0.0283 mmol/L was shown to be a good predictor of survival >1 year, and the most effective treatments for such patients were radio-frequency ablation (RFA) and transarterial chemoembolization (TACE) with radiation therapy (RT). In patients without this combination, the best treatments were RFA, TACE with RT and targeted drug therapy, and TACE with targeted drug therapy and immunotherapy. The main limitation of this study was its small sample. With a small sample size, we may have developed a less reliable model system, failing to produce any clinically important results or outcomes. Conclusion: Data mining can produce models to help clinicians predict survival time at the time of initial HCC diagnosis and then choose the most suitable treatment.
Association of Self-Reported Sleep Characteristics and Hip Fracture: Observational and Mendelian Randomization Studies
Authors: Wang Yan-Fei , Luo Yu-Feng , Mhalgi Asmi , Ren Wen-Yan , Wu Long-Fei
Published: March 2023

Previous observational studies on the relationship between sleep characteristics and fracture have yielded contradictory results. The goal of this study was to replicate the findings in a large longitudinal cohort and then conduct a Mendelian randomization (MR) analysis to infer the causality between sleep behaviors and fracture risk. Based on data from the China Health and Retirement Longitudinal Study (CHARLS) including 17,708 participants, we found that individuals with short sleep duration (<5 h) (OR [odds ratio] = 1.62, 95% CI: 1.07–2.44) or restless sleep (OR = 1.55, 95% CI: 1.10–2.19) have a higher risk of hip fracture. A U-shaped relationship between nighttime sleep duration and hip fracture risk (p-nonlinear = 0.01) was observed using restricted cubic spline regression analysis. Through joint effect analysis, we found that participants with short sleep duration (<5 h) combined with midday napping could significantly decrease hip fracture incidence. We further inferred the causal relationship between self-reported sleep behaviors and hip fracture using the MR approach. Among four sleep phenotypic parameters (sleep duration, daytime napping, chronotype, and insomnia), we found a modest causal relationship between sleep duration and fracture (OR = 0.69, 95% CI: 0.48 to 0.99, p = 0.04). However, no causal relationship was observed for other sleep traits. In conclusion, our findings suggest that short sleep duration has a potential detrimental effect on hip fracture. Improving sleep patterns is of significance for developing hip fracture preventive strategies in the middle-aged and the elderly populations.
Fluorescent Marker as a Tool to Improve Strategies to Control Contaminated Surfaces and Decrease Danger of Cross-Contamination in Dental Clinics, during and beyond the COVID-19 Pandemic
Authors: Dolev Eran , Eli Ilana , Mashkit Ester , Grinberg Naftali , Emodi-Perlman Alona
Published: March 2023

The COVID-19 pandemic posed an increased threat to dental personnel and patients. Close encounters with patients’ breath and saliva and the use of intraoral rotating instruments which disperse microscopic airborne particles both increase the possibility of environmental infection. In this study, fluorescent marker (FM) was used to assess and enhance surface cleanliness in the dental clinics and public areas of a major dental school. Initially, 574 surfaces in various areas of a dental school were marked with FM for 3 consecutive months to monitor the surface cleanliness. The initial evaluation results were visually presented to both students and para-dental and cleaning personnel during a designated educational session, and were used to stress the importance of preventing cross-contamination. Following educational intervention, 662 surfaces were re-examined for an additional 3 months, using the same method. A significant improvement in the surfaces’ cleanliness (ANOVA, F(1) = 10.89, p < 0.005) was observed post-intervention. The results were more prominent in students’ clinics, which were the students’ cleaning responsibility. The results show that fluorescent markers can serve as an educational tool to improve strategies to control contaminated surfaces in large clinics, such as dental schools. Their use can substantially decrease the hazard of cross-contamination during the pandemic and beyond.
Experience of Ethnic Discrimination, Anxiety, Perceived Risk of COVID-19, and Social Support among Polish and International Students during the Pandemic
Authors: Bokszczanin Anna , Gladysh Olga , Bronowicka Anna , Palace Marek
Published: March 2023

Background: Our research aimed to assess the experiences of ethnic discrimination among students in Poland (Polish and international) during the COVID-19 pandemic. We also tested the prevalence of anxiety symptoms and their relationship with perceived COVID-19 risk, the severity of discrimination, and social support. Methods: The data from Polish (n = 481) and international university students (n = 105) were collected online (November–January 2020). Participants completed measures of ethnic discrimination (GEDS), anxiety scale (GAD-7), COVID-19 risk perception index, and perceived social support scale (MSPSS) questionnaires. Results: The results showed that international students reported being much more discriminated than Polish students during the first year of the COVID-19 pandemic. Contrary to our expectation, a higher risk of anxiety disorders (GAD) was observed in 42% of Polish students compared to 31% of international students. The predictors of higher anxiety symptoms among both groups were the perceived risk of COVID-19 and the greater severity of ethnic discrimination. In both groups, the perceived social support had a protective role in anxiety symptomatology. Conclusions: The high prevalence of discrimination, especially among international students, simultaneously with high symptoms of anxiety, requires vigorous action involving preventive measures and psychological support.
Do CEO Attributes Spur Conservatism?
Authors: Atwa Rawan , Alsmadi Safaa , Kharabsheh Buthiena , Haddad Ruwaidah
Published: March 2023

This study examines the relationship between chief executive officers’ (CEOs’) characteristics (e.g., tenure, experience, education, age and compensation) and accounting conservatism for a sample of 672 yearly observations from both Jordanian industrial and service companies listed on the Amman Stock Exchange (ASE) during the period 2014–2021. Using feasible generalised least squares, the results show that CEOs with more experience and skills are positively and significantly related to accounting conservatism. Furthermore, consistent with upper-echelon-theory arguments, the findings reveal that CEO tenure is significantly and positively associated with the level of accounting conservatism. The results indicate that CEOs’ education, age and compensation are positively but insignificantly related to accounting conservatism. Overall, this study contributes to the literature by providing evidence of the importance of recognising the effects of CEOs’ characteristics on influencing accounting conservatism in Jordanian industrial and service companies.
Pre- and Post-COVID-19: The Impact of US, UK, and European Stock Markets on ASEAN-5 Stock Markets
Authors: Jamil Izaan , Kogid Mori , Lim Thien Sang , Lily Jaratin
Published: March 2023

This study investigates the relationship between closing–opening prices of stocks in the US, UK, and European markets and the prices of stocks in the five Association of Southeast Asian Nations (ASEAN-5) markets, a group consisting of five founding members, namely, Indonesia, Malaysia, the Philippines, Singapore, and Thailand. In particular, this study examines the impact of US, UK, and European stock market movements on ASEAN-5 stock markets before and during the COVID-19 pandemic. An autoregressive distributed lag (ARDL) bounds testing approach was employed on two independent data sets, representing prices of stocks before and during the COVID-19 pandemic. The results reveal that among the ASEAN-5 markets, only the Philippines had a cointegration relationship with the US, UK, and European markets before the crisis. However, almost all ASEAN-5 markets moved in tandem with the US, UK, and European markets during COVID-19, except for Thailand. These empirical findings also indicate that the stock markets in the two regions tended to co-move during the COVID-19 pandemic, implying a contagion effect. Further, the causality results also provide substantial evidence of contagion between markets during the pandemic. These results imply that the stock markets in ASEAN-5 are susceptible at the opening bell to the behaviour of US, UK, and European stocks. Therefore, investors or traders in ASEAN-5 should participate in foreign markets (other than the US, UK, and Europe) that do not exhibit cointegration relationships to better mitigate and manage risk at the opening bell, especially during a global crisis.
Spatial Analysis of the Suitability of Hass Avocado Cultivation in the Cauca Department, Colombia, Using Multi-Criteria Decision Analysis and Geographic Information Systems
Authors: Anacona Mopan Yesid Ediver , Solis Pino Andrés Felipe , Rubiano-Ovalle Oscar , Paz Helmer , Ramirez Mejia Isabel
Published: March 2023

Avocado is an important export and consumption product in Colombia, and its economic importance is expected to increase in the coming years. With its vast potential territory for avocado cultivation, the department of Cauca is a crucial area for producing this variety. However, small producers in the region often need more knowledge of the most suitable locations for planting. This study seeks to determine the ideal areas for Hass avocado cultivation in Cauca using geographic information tools and multi-criteria decision analysis, using a set of official data from different governmental entities and the hierarchical analytical process that allows determining the intensity of the interrelation of factors in the cultivation of Hass avocado. The results indicate that the municipalities near the Popayán plateau have the most significant potential for Hass avocado production, using the analytical hierarchy process. Approximately 9.2% of the administrative territory of the region is classified as highly suitable for Hass avocado cultivation, and an additional 14.2% is considered moderately suitable, constituting about 700,000 hectares of arable land. This research provides decision-makers and producers with valuable knowledge to support and improve Hass avocado agriculture in the region by implementing agricultural engineering practices.
Variation in Lipid Species Profiles among Leukemic Cells Significantly Impacts Their Sensitivity to the Drug Targeting of Lipid Metabolism and the Prognosis of AML Patients
Authors: Lo Presti Caroline , Yamaryo-Botté Yoshiki , Mondet Julie , Berthier Sylvie , Nutiu Denisa , Botté Cyrille , Mossuz Pascal
Published: March 2023

Several studies have linked bad prognoses of acute myeloid leukemia (AML) to the ability of leukemic cells to reprogram their metabolism and, in particular, their lipid metabolism. In this context, we performed “in-depth” characterization of fatty acids (FAs) and lipid species in leukemic cell lines and in plasma from AML patients. We firstly showed that leukemic cell lines harbored significant differences in their lipid profiles at steady state, and that under nutrient stress, they developed common mechanisms of protection that led to variation in the same lipid species; this highlights that the remodeling of lipid species is a major and shared mechanism of adaptation to stress in leukemic cells. We also showed that sensitivity to etomoxir, which blocks fatty acid oxidation (FAO), was dependent on the initial lipid profile of cell lines, suggesting that only a particular “lipidic phenotype” is sensitive to the drug targeting of FAO. We then showed that the lipid profiles of plasma samples from AML patients were significantly correlated with the prognosis of patients. In particular, we highlighted the impact of phosphocholine and phosphatidyl-choline metabolism on patients’ survival. In conclusion, our data show that balance between lipid species is a phenotypic marker of the diversity of leukemic cells that significantly influences their proliferation and resistance to stress, and thereby, the prognosis of AML patients.
Unraveling the Aquaporin-3 Inhibitory Effect of Rottlerin by Experimental and Computational Approaches
Authors: Paccetti-Alves Inês , Batista Marta S. P. , Pimpão Catarina , Victor Bruno L. , Soveral Graça
Published: March 2023

The natural polyphenolic compound Rottlerin (RoT) showed anticancer properties in a variety of human cancers through the inhibition of several target molecules implicated in tumorigenesis, revealing its potential as an anticancer agent. Aquaporins (AQPs) are found overexpressed in different types of cancers and have recently emerged as promising pharmacological targets. Increasing evidence suggests that the water/glycerol channel aquaporin-3 (AQP3) plays a key role in cancer and metastasis. Here, we report the ability of RoT to inhibit human AQP3 activity with an IC50 in the micromolar range (22.8 ± 5.82 µM for water and 6.7 ± 2.97 µM for glycerol permeability inhibition). Moreover, we have used molecular docking and molecular dynamics simulations to understand the structural determinants of RoT that explain its ability to inhibit AQP3. Our results show that RoT blocks AQP3-glycerol permeation by establishing strong and stable interactions at the extracellular region of AQP3 pores interacting with residues essential for glycerol permeation. Altogether, our multidisciplinary approach unveiled RoT as an anticancer drug against tumors where AQP3 is highly expressed providing new information to aquaporin research that may boost future drug design.
Tissue-Specific Transcriptome and Metabolome Analysis Reveals the Response Mechanism of Brassica napus to Waterlogging Stress
Authors: Hong Bo , Zhou Bingqian , Peng Zechuan , Yao Mingyao , Wu Junjie , Wu Xuepeng , Guan Chunyun , Guan Mei
Published: March 2023

During the growth period of rapeseed, if there is continuous rainfall, it will easily lead to waterlogging stress, which will seriously affect the growth of rapeseed. Currently, the mechanisms of rapeseed resistance to waterlogging stress are largely unknown. In this study, the rapeseed (Brassica napus) inbred lines G230 and G218 were identified as waterlogging-tolerant rapeseed and waterlogging-sensitive rapeseed, respectively, through a potted waterlogging stress simulation and field waterlogging stress experiments. After six days of waterlogging stress at the seedling stage, the degree of leaf aging and root damage of the waterlogging-tolerant rapeseed G230 were lower than those of the waterlogging-sensitive rapeseed G218. A physiological analysis showed that waterlogging stress significantly increased the contents of malondialdehyde, soluble sugar, and hydrogen peroxide in rape leaves and roots. The transcriptomic and metabolomic analysis showed that the differential genes and the differential metabolites of waterlogging-tolerant rapeseed G230 were mainly enriched in the metabolic pathways, biosynthesis of secondary metabolites, flavonoid biosynthesis, and vitamin B6 metabolism. Compared to G218, the expression levels of some genes associated with flavonoid biosynthesis and vitamin B metabolism were higher in G230, such as CHI, DRF, LDOX, PDX1.1, and PDX2. Furthermore, some metabolites involved in flavonoid biosynthesis and vitamin B6 metabolism, such as naringenin and epiafzelechin, were significantly up-regulated in leaves of G230, while pyridoxine phosphate was only significantly down-regulated in roots and leaves of G218. Furthermore, foliar spraying of vitamin B6 can effectively improve the tolerance to waterlogging of G218 in the short term. These results indicate that flavonoid biosynthesis and vitamin B6 metabolism pathways play a key role in the waterlogging tolerance and hypoxia stress resistance of Brassica napus and provide new insights for improving the waterlogging tolerance and cultivating waterlogging-tolerant rapeseed varieties.
Docking and Selectivity Studies of Covalently Bound Janus Kinase 3 Inhibitors
Authors: Zhong Haizhen A. , Almahmoud Suliman
Published: March 2023

The Janus kinases (JAKs) are a family of non-receptor cytosolic protein kinases critical for immune signaling. Many covalently bound ligands of JAK3 inhibitors have been reported. To help design selective JAK inhibitors, in this paper, we used five model proteins to study the subtype selectivity of and the mutational effects on inhibitor binding. We also compared the Covalent Dock programs from the Schrodinger software suite and the MOE software suite to determine which method to use for the drug design of covalent inhibitors. Our results showed that the docking affinity from 4Z16 (JAK3 wild-type model), 4E4N (JAK1), 4D1S (JAK2), and 7UYT (TYK2) from the Schrödinger software suite agreed well with the experimentally derived binding free energies with small predicted mean errors. However, the data from the mutant 5TTV model using the Schrödinger software suite yielded relatively large mean errors, whereas the MOE Covalent Dock program gave small mean errors in both the wild-type and mutant models for our model proteins. The docking data revealed that Leu905 of JAK3 and the hydrophobic residue at the same position in different subtypes (Leu959 of JAK1, Leu932 of JAK2, and Val981 of TYK2) is important for ligand binding to the JAK proteins. Arg911 and Asp912 of JAK3, Asp939 of JAK2, and Asp988 of TYK2 can be used for selective binding over JAK1, which contains Lys965 and Glu966 at the respective positions. Asp1021, Asp1039, and Asp1042 can be utilized for JAK1-selective ligand design, whereas Arg901 and Val981 may help guide TYK2-selective molecule design.
Genome-Wide Identification, Molecular Characterization, and Involvement in Response to Abiotic and Biotic Stresses of the HSP70 Gene Family in Turbot (Scophthalmus maximus)
Authors: Zheng Weiwei , Xu Xiwen , Chen Yadong , Wang Jing , Zhang Tingting , E Zechen , Chen Songlin , Liu Yingjie
Published: March 2023

Heat shock proteins 70 (HSP70s) are known to play essential roles in organisms’ response mechanisms to various environmental stresses. However, no systematic identification and functional analysis has been conducted for HSP70s in the turbot (Scophthalmus maximus), a commercially important worldwide flatfish. Herein, 16 HSP70 genes unevenly distributed on nine chromosomes were identified in the turbot at the genome-wide level. Analyses of gene structure, motif composition, and phylogenetic relationships provided valuable data on the HSP70s regarding their evolution, classification, and functional diversity. Expression profiles of the HSP70 genes under five different stresses were investigated by examining multiple RNA-seq datasets. Results showed that 10, 6, 8, 10, and 9 HSP70 genes showed significantly up- or downregulated expression after heat-induced, salinity-induced, and Enteromyxum scophthalmi, Vibrio anguillarum, and Megalocytivirus infection-induced stress, respectively. Among them, hsp70 (hspa1a), hspa1b, and hspa5 showed significant responses to each kind of induced stress, and qPCR analyses further validated their involvement in comprehensive anti-stress, indicating their involvement in organisms’ anti-stress mechanisms. These findings not only provide new insights into the biological function of HSP70s in turbot adapting to various environmental stresses, but also contribute to the development of molecular-based selective breeding programs for the production of stress-resistant turbot strains in the aquaculture industry.
Emerging Targeted Therapeutic Strategies to Overcome Imatinib Resistance of Gastrointestinal Stromal Tumors
Authors: Masucci Maria Teresa , Motti Maria Letizia , Minopoli Michele , Di Carluccio Gioconda , Carriero Maria Vincenza
Published: March 2023

Gastrointestinal stromal tumors (GISTs) are the most common malignant mesenchymal neoplasms of the gastrointestinal tract. The gold standard for the diagnosis of GISTs is morphologic analysis with an immunohistochemical evaluation plus genomic profiling to assess the mutational status of lesions. The majority of GISTs are driven by gain-of-function mutations in the proto-oncogene c-KIT encoding the tyrosine kinase receptor (TKR) known as KIT and in the platelet-derived growth factor-alpha receptor (PDGFRA) genes. Approved therapeutics are orally available as tyrosine kinase inhibitors (TKIs) targeting KIT and/or PDGFRA oncogenic activation. Among these, imatinib has changed the management of patients with unresectable or metastatic GISTs, improving their survival time and delaying disease progression. Nevertheless, the majority of patients with GISTs experience disease progression after 2–3 years of imatinib therapy due to the development of secondary KIT mutations. Today, based on the identification of new driving oncogenic mutations, targeted therapy and precision medicine are regarded as the new frontiers for GISTs. This article reviews the most important mutations in GISTs and highlights their importance in the current understanding and treatment options of GISTs, with an emphasis on the most recent clinical trials.