bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of B on hot ductility of high Al, TWIP steels
Authors: Kang S. E. , Banerjee J. R. , Tuling A. S. , Mintz B.
Published: October 2013

The influence of B on the hot ductility of high Al, Ti containing twinning induced plasticity (TWIP) steels has been examined. It was established that provided the B was fully protected by adding sufficient Ti to combine with all the N, then B could segregate to the austenite grain boundaries and improve ductility. This improvement was particularly marked for the temperature range of 700–900°C, the range in which the straightening operation often takes place in continuous casting. Of most importance in the present work has been the detection of B at the boundaries using a secondary ion mass spectrometry technique. The cooling rate from the reheating temperature of 1250°C to the tensile testing temperature range of 700–1200°C was 60 K min−1, but it is likely that slower cooling rates ≤25 K min−1, more in keeping with the secondary cooling rate on continuous casting, will give even better ductility. Ti additions in themselves are beneficial to the hot ductility of these steels as precipitation of AlN at the austenite boundaries is avoided, but only if the cooling rate is sufficiently slow to allow the TiN particles to coarsen. However, to ensure freedom from cracking, an addition of B is also required.
Microscale analysis of influence of wettability on flow behaviours of molten Cu infiltrating into W channel
Authors: Bai Y. X. , Liang S. H.
Published: October 2013

In order to better understand the effect of the wettability between Cu and W on the infiltration process during the fabrication of CuW alloy, the flow patterns of molten Cu in the W microchannel at four different contact angles between Cu and W were simulated by finite volume method based on the modified Navier–Stokes equation, and the effect of Co content in molten Cu on the flow front was studied as well. The results show that the less contact angle between Cu and W increases the differential and negative pressures generated at the flow front and thus increases the flow velocity and decreases the infiltration time during the fabrication process of CuW alloy. However, the less contact angle is easier to cause a larger negative pressure with the progress of the infiltration process, which is not favourable for the filling of molten Cu in the W channel. In addition, Co addition can accelerate the flow of molten Cu in the W channel due to the increased differential pressure.
Electrodeposition of SnBi coatings based on deep eutectic solvent
Authors: Gao Y. , Hu W. , Gao X. , Duan B.
Published: October 2013

Tin–bismuth (SnBi) alloy coatings were satisfactorily electrodeposited on copper electrodes from a choline chloride–ethylene glycol deep eutectic solvent containing BiCl3, ZnCl2 and H3BO3. The electrochemical feature of the electrolyte system was inspected via linear sweep voltammetry. The results indicated that the SnBi alloy coating was codeposited as the deposition potential was prolonged up to the potential range, leading to the inadequate deposition of zinc on the copper electrode. Dendrite formation, simple eutectics and diversification of grain size were studied through the results of the morphology and structure analyses. The X-ray diffraction results demonstrated an increasing grain size of Bi and an opposite trend of Sn when the potential increased. The relevance of the Bi at% of the codeposited BiSn on the deposition potential, temperature and Bi(II) molar ratio was investigated. The results indicated the possibility of electroplating the SnBi alloys with their composition around the eutectic point.
On deposition of Waspaloy coatings by cold spray
Authors: Vezzù S. , Rech S. , Vedelago E. , Zanon G. P. , Alfeo G. , Scialpi A. , Huang R.
Published: October 2013

The deposition of pore free and highly adhered Ni and Co superalloy coatings is of great interest for engine design and gas turbine applications, both in case of maintenance repair and overhaul operations as well for mechanical and chemical protection purposes in aeronautics and energy applications. This study would like to give a wide overview about the capability of cold spray technology on this topic: two different commercially available deposition systems, Sulzer-CGT Kinetiks 4000 and Plasma Giken PCS-1000, were compared, and deposition processes with both nitrogen and helium as carrier gas have been explored. Microstructural investigation, microhardness and adhesion results are reported to depict a preliminary scenario of coating properties. Fully dense coatings with thickness >1 mm and adhesion >50 MPa are obtained using helium, while quite porous, 20 MPa adhered coatings are obtained using nitrogen. Finally, general considerations about the potential applicability of those coatings for repair purpose in aerospace applications are pointed out.
Potential association of pulmonary tuberculosis with genetic polymorphisms of toll-like receptor 9 and interferon-gamma in a Chinese population
Authors: Yang Yu , Li Xiangwei , Cui Wei , Guan Ling , Shen Fei , Xu Jinsheng , Zhou Feng , Li Mufei , Gao Cong , Jin Qi , Liu Jianmin , Gao Lei
Published: October 2013

Background

Association studies have been employed to investigate the relationships between host single nucleotide polymorphisms (SNPs) and susceptibility to pulmonary Tuberculosis (PTB). However, such candidate genetic markers have not been widely studied in Chinese population, especially with respect to the disease development from latent M. tuberculosis infection (LTBI).

Methods

In this case–control study, 44 candidate SNPs were examined in a total of 600 participants (PTB patients, LTBI controls and healthy controls without M. tuberculosis infection) from Zhengzhou, China. The two groups of controls were frequency matched on gender and age with PTB patients. Genotyping was carried out by the Illumina Golden Gate assay.

Results

When comparing PTB patients with LTBI controls but not healthy controls without M. tuberculosis infection, significant associations with disease development were observed for TLR9 1174 A/G, TLR9 1635 A/G and IFNG 2109G/A. The two loci in TLR9 were in LD in our study population (r2=0.96, D’=1.00). A combined effect of the genotypes associated with increased risk of PTB (i.e. TLR9 1174G/G and IFNG 2109 A/A) was found when comparing PTB patients with LTBI controls (p=0.004) but not with healthy controls without infection (p=0.433).

Conclusions

Potential associations between TLR9 and IFN-γ genetic polymorphisms and PTB were observed in a Chinese population which supports further study of the roles played by TLR9/IFN-γ pathway during the development of PTB.
Oral versus intravenous antibiotics for patients with Klebsiella pneumoniae liver abscess: study protocol for a randomized controlled trial
Authors: Molton James , Phillips Rachel , Gandhi Mihir , Yoong Joanne , Lye David , Tan Thuan Tong , Fisher Dale , Archuleta Sophia
Published: October 2013

Background

Klebsiella pneumoniae liver abscess is the most common etiology of liver abscess in Singapore and much of Asia, and its incidence is increasing. Current management includes prolonged intravenous antibiotic therapy, but there is limited evidence to guide oral conversion. The implicated K1/K2 capsule strain of Klebsiella pneumoniae is almost universally susceptible to ciprofloxacin, an antibiotic with high oral bioavailability. Our primary aim is to compare the efficacy of early (< one week) step-down to oral antibiotics, to continuing four weeks of intravenous antibiotics, in patients with Klebsiella liver abscess.

Methods/design

The study is designed as a multi-center randomized open-label active comparator-controlled non-inferiority trial, with a non-inferiority margin of 12%. Eligible participants will be inpatients over the age of 21 with a CT or ultrasound scan suggestive of a liver abscess, and Klebsiella pneumoniae isolated from abscess fluid or blood. Randomization into intervention or active control arms will be performed with a 1:1 allocation ratio. Participants randomized to active control will receive IV ceftriaxone 2 grams daily to complete a total of four weeks of IV antibiotics. Participants randomized to intervention will be immediately converted to oral ciprofloxacin 750 mg twice daily. At Week four, all participants will undergo abdominal imaging and be assessed for clinical response (CRP < 20 mg/l, absence of fever, plus scan showing that the maximal diameter of the abscess has reduced). If criteria are met, antibiotics are stopped; if not, oral antibiotics are continued, with reassessment for clinical response fortnightly. If criteria for clinical response are met by Week 12, the primary endpoint of clinical cure is met. A cost analysis will be performed to assess the cost saving of early conversion to oral antibiotics, and a quality of life analysis will be performed to assess whether treatment with oral antibiotics is less burdensome than prolonged IV antibiotics.

Discussion

Our results would help inform local and international practice guidelines regarding the optimal antibiotic management of Klebsiella liver abscess. A finding of non-inferiority may translate to the wider adoption of a more cost-effective strategy that reduces hospital length of stay and improves patient-centered outcomes and satisfaction.

Trial registration

Clinical trials gov NCT01723150
Accelerometer measured daily physical activity and sedentary pursuits–comparison between two models of the Actigraph and the importance of data reduction
Authors: Tanha Tina , Tornberg Åsa , Dencker Magnus , Wollmer Per
Published: October 2013

Background

Very few validation studies have been performed between different generations of the commonly used Actigraph accelerometers. We compared daily physical activity data generated from the old generation Actigraph model 7164 with the new generation Actigraph GT1M accelerometer in 15 young females for eight consecutive days. We also investigated if different wear time thresholds had any impact on the findings. Minutes per day of moderate and vigorous physical activity (MVPA), vigorous physical activity (VPA) and very vigorous physical activity (VVPA) were calculated. Moreover, minutes of sedentary pursuits per day were calculated.

Findings

There were significant (P < 0.05) differences between the Actigraph 7164 and the GT1M concerning MVPA (61 ± 21vs. 56 ± 23 min/day), VPA (12 ± 8 vs. 9 ± 3 min/day) and VVPA (3.2 ± 3.0 vs. 0.3 ± 1.1 min/day). The different wear time thresholds had little impact on minutes per day in different intensities. Median minutes of sedentary pursuits per day ranged from 159 to 438 minutes depending on which wear time threshold was used (i.e. 10, 30 or 60 minutes), whereas very small differences were observed between the two different models.

Conclusions

Data from the old generation Actigraph 7164 and the new generation Actigraph GT1M accelerometers differ, where the Actigraph GT1M generates lower minutes spent in free living physical activity. Median minutes of sedentary pursuits per day are highly dependent on which wear time threshold that is used, and not by accelerometer model.
Detection and identification of the phytoplasma associated with China ixeris (Ixeridium chinense) fasciation
Authors: Li Zheng-Nan , Liu Ping , Zhang Lei , Wu Yun-Feng
Published: October 2013

Background

Phytoplasmas are always associated with symptoms in host plants such as stunting of stems, witches’-broom, yellowing of leaves, formation of sterile-deformed flowers, virescence and phyllody. Recently also symptom of fasciation was reported associated with phytoplasma presence. In the present work, China ixeris fasciation was observed associated with phytoplasmas in Guanzhong Area, Shaanxi, China.

Results

Phytoplasma-like bodies were observed under transmission electron microscope in stem tissues of symptomatic samples. The 16S rRNA operon and tuf genes from phytoplasmas were amplified by PCR assays. Phylogenetic trees were calculated respectively based on sequences data of these two genes. The pattern of restriction fragment length polymorphism (RFLP) was generated via digesting the PCR products of 16S rRNA gene with eight restriction enzymes.

Conclusion

The presence of phytoplasma in China ixeris exhibiting fasciation symptom was confirmed by the results of TEM observation and PCR testing. Based on sequence data, phylogeny analysis and actual restriction fragment length polymorphism (RFLP) analysis, the associated phytoplasma was classified as related to 16SrI-C subgroup. This was the first record of phytoplasmas in China ixeris.
Bone ultrasound velocity in pediatric intensive care unit: a pilot study
Authors: Zerem Ayelet , Mimouni Francis B , Picard Elie , Shahroor Sarit
Published: October 2013

Background

Bone loss has been documented in adults in intensive care wards. Children admitted to pediatric intensive care units (PICU) are also exposed to many potential risk factors for bone loss such as immobilization, catabolic state, and nutritional depletion. Quantitative ultrasound technique that measures speed of sound (SOS) correlates with bone mineral density (BMD) and strength. Herein is a clinical prospective longitudinal, observational pilot study to evaluate early bone changes that occur during the first few days of PICU admission.

Methods

Children are hospitalized in a pediatric intensive under general anesthesia and muscle paralysis. Bone SOS at the mid-shaft tibia was measured on the first day of hospitalization and on days 2 to 3 thereafter.

Results

Nineteen children were studied. Bone SOS decreased during the first 3 days of hospitalization from 3,297 ± 315 to 3,260 ± 311 m/min (p < 0.05). The decrease was approximately 1% of the original SOS over the first 2 to 3 days of admission.

Conclusion

There is a significant decrease in bone strength after 3 days in pediatric patients admitted to an intensive care department. Longitudinal studies of a larger group of children are necessary to determine the clinical meaning of the results and to possibly evaluate preventive approaches.
Obesity associated alterations in the biology of adipose stem cells mediate enhanced tumorigenesis by estrogen dependent pathways
Authors: Strong Amy L , Strong Thomas A , Rhodes Lyndsay V , Semon Julie A , Zhang Xiujuan , Shi Zhenzhen , Zhang Shijia , Gimble Jeffrey M , Burow Matthew E , Bunnell Bruce A
Published: October 2013

Introduction

Obesity has been associated with increased incidence and mortality of breast cancer. While the precise correlation between obesity and breast cancer remains to be determined, recent studies suggest that adipose tissue and adipose stem cells (ASCs) influence breast cancer tumorigenesis and tumor progression.

Methods

Breast cancer cells lines were co-cultured with ASCs (n = 24), categorized based on tissue site of origin and body mass index (BMI), and assessed for enhanced proliferation, alterations in gene expression profile with PCR arrays, and enhanced tumorigenesis in immunocompromised mice. The gene expression profile of ASCs was assess with PCR arrays and qRT-PCR and confirmed with Western blot analysis. Inhibitory studies were conducted by delivering estrogen antagonist ICI182,780, leptin neutralizing antibody, or aromatase inhibitor letrozole and assessing breast cancer cell proliferation. To assess the role of leptin in human breast cancers, Oncomine and Kaplan Meier plot analyses were conducted.

Results

ASCs derived from the abdominal subcutaneous adipose tissue of obese subjects (BMI > 30) enhanced breast cancer cell proliferation in vitro and tumorigenicity in vivo. These findings were correlated with changes in the gene expression profile of breast cancer cells after co-culturing with ASCs, particularly in estrogen receptor-alpha (ESR1) and progesterone receptor (PGR) expression. Analysis of the gene expression profile of the four groups of ASCs revealed obesity induced alterations in several key genes, including leptin (LEP). Blocking estrogen signaling with ICI182,780, leptin neutralizing antibody, or letrozole diminished the impact of ASCs derived from obese subjects. Women diagnosed with estrogen receptor/progesterone receptor positive (ER+/PR+) breast cancers that also expressed high levels of leptin had poorer prognosis than women with low leptin expression.

Conclusion

ASCs isolated from the abdomen of obese subjects demonstrated increased expression of leptin, through estrogen stimulation, which increased breast cancer cell proliferation. The results from this study demonstrate that abdominal obesity induces significant changes in the biological properties of ASCs and that these alterations enhance ER+/PR+ breast cancer tumorigenesis through estrogen dependent pathways.
Early diagnosis of sepsis-related hepatic dysfunction and its prognostic impact on survival: a prospective study with the LiMAx test
Authors: Kaffarnik Magnus F , Lock Johan F , Vetter Hannah , Ahmadi Navid , Lojewski Christian , Malinowski Maciej , Neuhaus Peter , Stockmann Martin
Published: October 2013

Introduction

Liver dysfunction can derive from severe sepsis and might be associated with poor prognosis. However, diagnosis of septic liver dysfunction is challenging due to a lack of appropriate tests. Measurement of maximal liver function capacity (LiMAx test) has been successfully evaluated as a new diagnostic test in liver resection and transplantation. The aim of this study was to evaluate the LiMAx test during sepsis in comparison to biochemical tests and the indocyanin green test (ICG-PDR).

Methods

We prospectively investigated 28 patients (8 female and 20 male, age range 35 to 80 years) suffering from sepsis on a surgical ICU. All patients received routine resuscitation from septic shock (surgery, fluids, catecholamines, antibiotic drugs). The first LiMAx test and ICG-PDR were carried out within the first 24 hours after onset of septic symptoms, followed by day 2, 5 and 10. Other biochemical parameters and scores determining the severity of illness were measured daily. Clinical outcome parameters were examined after 90 days or at the end of treatment. The population was divided into 2 groups (group A: non-survivors or ICU length of stay (ICU-LOS) >30 days versus group B: survivors and ICU-LOS <30 days) for analysis.

Results

Epidemiological baseline characteristics of both groups were similar. Group A patients had significant lower LiMAx and ICG-PDR values than patients in group B. Determination of ICG-PDR by finger probe failed in 14.3% of tests due to insufficient peripheral pulses. Respiratory, renal and hepatic dysfunction (LiMAx and ICG-PDR) were associated with prolonged ICU-LOS. Only LiMAx <100 μg/kg/h and respiratory dysfunction were associated with increased mortality. For LiMAx <100 μg/kg/h receiver operating characteristic-analysis revealed a 100% sensitivity and 77% specificity for death.

Conclusions

Sepsis-related hepatic dysfunction can be diagnosed early and effectively by the LiMAx test. The extent of LiMAx impairment is predictive for patient morbidity and mortality. The sensitivity and specificity of the LiMAx test was superior to that of ICG-PDR regarding the prediction of mortality.
Serum activin A and B levels predict outcome in patients with acute respiratory failure: a prospective cohort study
Authors: de Kretser David Morritz , Bensley Jonathan Guy , Pettilä Ville , Linko Rita , Hedger Mark Peter , Hayward Susan , Allan Carolyn Anne , McLachlan Robert Ian , Ludlow Helen , Phillips David James
Published: October 2013

Introduction

30 day mortality in patients with Acute Respiratory Failure (ARF) is approximately 30%, defined as patients requiring ventilator support for more than 6 hours. Novel biomarkers are needed to predict patient outcomes and to guide potential future therapies. The activins A and B, members of the Transforming Growth Factor β family of proteins, and their binding protein, follistatin, have recently been shown to be important regulators of inflammation and fibrosis but no substantial data are available concerning their roles in ARF.

Our objectives were to evaluate whether the serum levels of activin A, B and follistatin are elevated in 518 patients with ARF from the FINNALI study compared the concentrations in 138 normal subjects that form a reference range.

Methods

Specific assays for activin A, B and follistatin were used and the results analyzed according to diagnostic groups as well as according to standard measures in intensive care. Multivariable logistic regression was used to create a model to predict death at 90 days and 12 months from the onset of the ARF.

Results

Serum activin A and B were significantly elevated in most patients and in most of the diagnostic groups. Patients who had activin A and/or B concentrations above the reference maximum were significantly more likely to die in the 12 months following admission [either activin A or B above reference maximum: Positive Likelihood Ratio [LR+] 1.65 [95% CI 1.28-2.12, P = 0.00013]; both activin A and B above reference maximum: LR + 2.78 [95% CI 1.96-3.95, P < 0.00001]. The predictive model at 12 months had an overall accuracy of 80.2% [95% CI 76.6-83.3%].

Conclusions

The measurement of activin A and B levels in these patients with ARF would have assisted in predicting those at greatest risk of death. Given the existing data from animal studies linking high activin A levels to significant inflammatory challenges, the results from this study suggest that approaches to modulate activin A and B bioactivity should be explored as potential therapeutic agents.
Coupled Soil-Structure Interaction Effects of Building Clusters during Earthquakes
Authors: Isbiliroglu Yigit , Taborda Ricardo , Bielak Jacobo
Published: October 2013

This study addresses the responses of idealized building clusters during earthquakes, their effects on ground motion, and the ways individual buildings interact with the soil and with each other. We simulate the ground motion during the 1994 Northridge earthquake and focus on the coupled responses of multiple simplified building models located within the San Fernando Valley. Numerical results show that the soil-structure interaction (SSI) effects vary with the number and dynamic properties of the buildings, their separation, and their impedance with respect to the soil. These effects appear as: (i) an increased spatial variability of the ground motion; and (ii) significant reductions in the buildings’ base motion at high frequencies, changes in the higher natural frequencies of the building-foundation systems, and variations in the roof displacement, with respect to those of the corresponding rigid-base and single SSI models.
The vertebrate-specific Kinesin-6, Kif20b, is required for normal cytokinesis of polarized cortical stem cells and cerebral cortex size
Authors: Janisch Kerstin M. , Vock Vita M. , Fleming Michael S. , Shrestha Ayushma , Grimsley-Myers Cynthia M. , Rasoul Bareza A. , Neale Sarah A. , Cupp Timothy D. , Kinchen Jason M. , Liem Karel F. , Dwyer Noelle D.
Published: October 2013

Mammalian neuroepithelial stem cells divide using a polarized form of cytokinesis, which is not well understood. The cytokinetic furrow cleaves the cell by ingressing from basal to apical, forming the midbody at the apical membrane. The midbody mediates abscission by recruiting many factors, including the Kinesin-6 family member Kif20b. In developing embryos, Kif20b mRNA is most highly expressed in neural stem/progenitor cells. A loss-of-function mutant in Kif20b, magoo, was found in a forward genetic screen. magoo has a small cerebral cortex, with reduced production of progenitors and neurons, but preserved layering. In contrast to other microcephalic mouse mutants, mitosis and cleavage furrows of cortical stem cells appear normal in magoo. However, apical midbodies show changes in number, shape and positioning relative to the apical membrane. Interestingly, the disruption of abscission does not appear to result in binucleate cells, but in apoptosis. Thus, Kif20b is required for proper midbody organization and abscission in polarized cortical stem cells and has a crucial role in the regulation of cerebral cortex growth.
TheDrosophilaubiquitin-specific protease Puffyeye regulates dMyc-mediated growth
Authors: Li Ling , Anderson Sarah , Secombe Julie , Eisenman Robert N.
Published: October 2013

The essential and highly conserved role of Myc in organismal growth and development is dependent on the control of Myc protein abundance. It is now well established that Myc levels are in part regulated by ubiquitin-dependent proteasomal degradation. Using a genetic screen for modifiers of Drosophila Myc (dMyc)-induced growth, we identified and characterized a ubiquitin-specific protease (USP), Puffyeye (Puf), as a novel regulator of dMyc levels and function in vivo. We show that puf genetically and physically interacts with dMyc and the ubiquitin ligase archipelago (ago) to modulate a dMyc-dependent cell growth phenotype, and that varying Puf levels in both the eye and wing phenocopies the effects of altered dMyc abundance. Puf containing point mutations within its USP enzymatic domain failed to alter dMyc levels and displayed no detectable phenotype, indicating the importance of deubiquitylating activity for Puf function. We find that dMyc induces Ago, indicating that dMyc triggers a negative-feedback pathway that is modulated by Puf. In addition to its effects on dMyc, Puf regulates both Ago and its cell cycle substrate Cyclin E. Therefore, Puf influences cell growth by controlling the stability of key regulatory proteins.
Genome-wide analysis of the bHLH gene family in planarians identifies factors required for adult neurogenesis and neuronal regeneration
Authors: Cowles Martis W. , Brown David D. R. , Nisperos Sean V. , Stanley Brianna N. , Pearson Bret J. , Zayas Ricardo M.
Published: October 2013

In contrast to most well-studied model organisms, planarians have a remarkable ability to completely regenerate a functional nervous system from a pluripotent stem cell population. Thus, planarians provide a powerful model to identify genes required for adult neurogenesis in vivo. We analyzed the basic helix-loop-helix (bHLH) family of transcription factors, many of which are crucial for nervous system development and have been implicated in human diseases. However, their potential roles in adult neurogenesis or central nervous system (CNS) function are not well understood. We identified 44 planarian bHLH homologs, determined their patterns of expression in the animal and assessed their functions using RNAi. We found nine bHLHs expressed in stem cells and neurons that are required for CNS regeneration. Our analyses revealed that homologs of coe, hes (hesl-3) and sim label progenitors in intact planarians, and following amputation we observed an enrichment of coe+ and sim+ progenitors near the wound site. RNAi knockdown of coe, hesl-3 or sim led to defects in CNS regeneration, including failure of the cephalic ganglia to properly pattern and a loss of expression of distinct neuronal subtype markers. Together, these data indicate that coe, hesl-3 and sim label neural progenitor cells, which serve to generate new neurons in uninjured or regenerating animals. Our study demonstrates that this model will be useful to investigate how stem cells interpret and respond to genetic and environmental cues in the CNS and to examine the role of bHLH transcription factors in adult tissue regeneration.
Measuring the Environmental Benefits of Wind-Generated Electricity
Authors: Cullen Joseph
Published: October 2013

Production subsidies for renewable energy, such as solar or wind power, are rationalized by their environmental benefits. Subsidizing these projects allows clean, renewable technologies to produce electricity that otherwise would have been produced by dirtier, fossil-fuel power plants. In this paper, I quantify the emissions offset by wind power for a large electricity grid in Texas using the randomness inherent in wind power availability. When accounting for dynamics in the production process, the results indicate that only for high estimates of the social costs of pollution does the value of emissions offset by wind power exceed cost of renewable energy subsidies. (JEL L94, L98, Q42, Q48, Q51, Q53, Q54)
Quasiconvexity equals lamination convexity for isotropic sets of 2 × 2 matrices
Authors: Heinz Sebastian
Published: October 2013

Let K be a given compact set of real 2×2 matrices that is isotropic, meaning invariant under the left and right action of the special orthogonal group. Then we show that the quasiconvex hull of K coincides with the lamination convex hull of order 2. In particular, there is no difference between quasiconvexity, rank-one convexity and lamination convexity for K. This is a generalization of a known result for connected sets.
China and Japan in “ASEAN Plus” Multilateral Arrangements
Authors: Chung Chien-peng
Published: October 2013

Interactions between China and Japan in multilateral “ASEAN plus dialogue partners” forums––the economic groupings ASEAN Plus Three and East Asia Summit, and the security groupings ASEAN Regional Forum and ASEAN Defense Ministerial Meeting Plus––have moved from attempts at cooperation, to competition for influence, and thence to attentiveness or disinterest.
Analysis of the roles of mutations in thyroid hormone receptor-β by a bacterial biosensor system
Authors: Shi Changhua , Meng Qing , Wood David W
Published: October 2013

Mutations in thyroid hormone receptors (TRs) often lead to metabolic and developmental disorders, but patients with these mutations are difficult to treat with existing thyromimetic drugs. In this study, we analyzed six clinically observed mutations in the ligand-binding domain of the human TRβ using an engineered bacterial hormone biosensor. Six agonist compounds, including triiodothyronine (T3), thyroxine (T4), 3,5,3′-triiodothyroacetic acid (Triac), GC-1, KB-141, and CO-23, and the antagonist NH-3 were examined for their ability to bind to each of the TRβ mutants. The results indicate that some mutations lead to the loss of ability to bind to native ligands, ranging from several fold to several hundred fold, while other mutations completely abolish the ability to bind to any ligand. Notably, the effect of each ligand on each TRβ mutant in this bacterial system is highly dependent on both the mutation and the ligand; some ligands were bound well by a wide variety of mutants, while other ligands lost their affinity for all but the WT receptor. This study demonstrates the ability of our bacterial system to differentiate agonist compounds from antagonist compounds and shows that one of the TRβ mutations leads to an unexpected increase in antagonist ability relative to other mutations. These results indicate that this bacterial sensor can be used to rapidly determine ligand-binding ability and character for clinically relevant TRβ mutants.
Rapid Recombination Mapping for High-Throughput Genetic Screens in Drosophila
Authors: Sapiro Anne L , Ihry Robert J , Buhr Derek L , Konieczko Kevin M , Ives Sarah M , Engstrom Anna K , Wleklinski Nicholas P , Kopish Kristin J , Bashirullah Arash
Published: October 2013

Mutagenesis screens are a staple of classical genetics. Chemical-induced mutations, however, are often difficult and time-consuming to identify. Here, we report that recombination analysis with pairs of dominant visible markers provides a rapid and reliable strategy to map mutations in Drosophila melanogaster. This method requires only two generations and a total of six crosses in vials to estimate the genetic map position of the responsible lesion with high accuracy. This genetic map position can then be reliably used to identify the mutated gene through complementation testing with an average of nine deficiencies and Sanger sequencing. We have used this approach to successfully map a collection of mutations from an ethyl methanesulfonate−based mutagenesis screen on the third chromosome. We propose that this method also may be used in conjunction with whole-genome sequencing, particularly when multiple independent alleles of the mutated locus are not available. By facilitating the rapid identification of mutated genes, our mapping strategy removes a primary obstacle to the widespread use of powerful chemical mutagenesis screens to understand fundamental biological phenomena.
Inducible Protein Traps with Dominant Phenotypes for Functional Analysis of theDrosophilaGenome
Authors: Singari Swetha , Javeed Naureen , Tardi Nicholas J , Marada Suresh , Carlson Jeff C , Kirk Steven , Thorn Judith M , Edwards Kevin A
Published: October 2013

The Drosophila melanogaster genome has been extensively characterized, but there remains a pressing need to associate gene products with phenotypes, subcellular localizations, and interaction partners. A multifunctional, Minos transposon-based protein trapping system called Hostile takeover (Hto) was developed to facilitate in vivo analyses of endogenous genes, including live imaging, purification of protein complexes, and mutagenesis. The Hto transposon features a UAS enhancer with a basal promoter, followed by an artificial exon 1 and a standard 5′ splice site. Upon GAL4 induction, exon 1 can splice to the next exon downstream in the flanking genomic DNA, belonging to a random target gene. Exon 1 encodes a dual tag (FLAG epitope and mCherry red fluorescent protein), which becomes fused to the target protein. Hto was mobilized throughout the genome and then activated by eye-specific GAL4; an F1 screen for abnormal eye phenotypes was used to identify inserts that express disruptive fusion proteins. Approximately 1.7% of new inserts cause eye phenotypes. Of the first 23 verified target genes, 21 can be described as regulators of cell biology and development. Most are transcription factor genes, including AP-2, CG17181, cut, klu, mamo, Sox102F, and sv. Other target genes [l(1)G0232, nuf, pum, and Syt4] make cytoplasmic proteins, and these lines produce diverse fluorescence localization patterns. Hto permits the expression of stable carboxy-terminal subfragments of proteins, which are rarely tested in conventional genetic screens. Some of these may disrupt specific cell pathways, as exemplified by truncated forms of Mastermind and Nuf.
Crossover Heterogeneity in the Absence of Hotspots inCaenorhabditis elegans
Authors: Kaur Taniya , Rockman Matthew V
Published: October 2013

Crossovers play mechanical roles in meiotic chromosome segregation, generate genetic diversity by producing new allelic combinations, and facilitate evolution by decoupling linked alleles. In almost every species studied to date, crossover distributions are dramatically nonuniform, differing among sexes and across genomes, with spatial variation in crossover rates on scales from whole chromosomes to subkilobase hotspots. To understand the regulatory forces dictating these heterogeneous distributions a crucial first step is the fine-scale characterization of crossover distributions. Here we define the wild-type distribution of crossovers along a region of the C. elegans chromosome II at unprecedented resolution, using recombinant chromosomes of 243 hermaphrodites and 226 males. We find that well-characterized large-scale domains, with little fine-scale rate heterogeneity, dominate this region’s crossover landscape. Using the Gini coefficient as a summary statistic, we find that this region of the C. elegans genome has the least heterogeneous fine-scale crossover distribution yet observed among model organisms, and we show by simulation that the data are incompatible with a mammalian-type hotspot-rich landscape. The large-scale structural domains—the low-recombination center and the high-recombination arm—have a discrete boundary that we localize to a small region. This boundary coincides with the arm-center boundary defined both by nuclear-envelope attachment of DNA in somatic cells and GC content, consistent with proposals that these features of chromosome organization may be mechanical causes and evolutionary consequences of crossover recombination.
Assumptions, challenges, and future directions in cumulative impact analysis
Authors: Halpern Benjamin S. , Fujita Rod
Published: October 2013

Efforts to understand and map cumulative impacts of human activities on ecosystems have gained new interest and relevance as management moves towards ecosystem‐based approaches that require such assessments. The last five years have seen a proliferation of efforts to characterize and map cumulative impacts, providing insight into the strengths and limitations of these efforts and where opportunities lie for progress. Here we provide a review of the key assumptions that underlie most cumulative impact mapping efforts, describing the implications and rationales for the assumptions, and highlight the many challenges cumulative impact mapping efforts face. We end with a brief summary of several future research directions that will help greatly improve application of cumulative impact mapping to resource management and conservation planning efforts.
Microwave Effects on Soluble Substrate and Thermophilic Digestibility of Activated Sludge
Authors: Coelho Nuno Miguel Gabriel , Droste Ronald L. , Kennedy Kevin J.
Published: October 2013

Thickened waste‐activated sludge (TWAS) was subjected to microwave pretreatment and athermal irradiation. The soluble phase of each type of TWAS pretreatment was subject to ultrafiltration in series using progressively smaller pore‐size membranes (300, 100, 10, and 1 kDa) and biodegradability tests. Microwave pretreatment solubilizes a considerable amount of the suspended organic substrate, but athermal irradiation also causes solubilization of organic matter, although at a smaller scale than microwave. Proteins are particularly sensitive to athermal irradiation, and both microwave and athermal irradiation are capable of changing the size distribution of dissolved organic matter. Athermal irradiation and microwave have a substantially different effect on thermophilic anaerobic biodegradability of the various size fractions obtained after ultrafiltration. Slight inhibition and decrease in total biogas production was measured in some microwave tests. Athermal irradiation does not cause a decrease in maximum biogas production rate in any test and increases slightly biogas production.
The Use of Multiple Tracers to Evaluate the Impact of Sewered and Non‐Sewered Development on Coastal Water Quality in a Rural Area of Florida
Authors: Meeroff Daniel E. , Bloetscher Frederick , Long Sharon C. , Bocca Thais
Published: October 2013

When onsite wastewater treatment and disposal systems (OSTDS) are not sited appropriately or installed properly, wastewater constituents can be a source of adverse environmental impacts to soil and groundwater, which can lead to potential public health risks. A paired monitoring design developed to compare water quality in sewered and non‐sewered areas is presented here. It is suggested as a possible monitoring scheme for assessing the impact of sewer installation projects. As such, two sets of single‐family, rural residential Florida neighborhoods were evaluated over a two‐year period to gain insight into the effects of small‐community use of OSTDS on coastal water quality. One set of two neighborhoods were connected to the sanitary sewer network and the other set of two were served exclusively by OSTDS. Water quality sampling was conducted at the paired sites during seasonal high water table (SHWT) and seasonal low water table (SLWT) events. Measured surface water quality during the SHWT showed indications of environmental impacts from OSTDS in terms of nutrients, microbial pathogen indicators, and other water quality measures, such as turbidity and conductivity. However, during the SLWT events, no obvious impacts attributable to OSTDS were detected. The water quality results indicate that OSTDS impacts may be measureable in rural areas. Other factors, such as microbial indicator survival and regrowth potential, may confound the understanding of water quality impacts of sewer projects. For example, the microbial indicators Escherichia coli and enterococci were found to persist over time and therefore did not always represent true comparisons of OSTDS and sewered areas between seasons. The timeframe for evaluating the effects of sewer projects may be longer than anticipated because of this survival and regrowth phenomenon.
Evaluation of Activated Sludge for Biodegradation of Propylene Glycol as an Aircraft Deicing Fluid
Authors: Delorit Justin D. , Racz LeeAnn
Published: October 2013

Aircraft deicing fluid used at airport facilities is often collected for treatment or disposal in order to prevent serious ecological threats to nearby surface waters. This study investigated lab scale degradation of propylene glycol, the active ingredient in a common aircraft deicing fluid, by way of a laboratory‐scale sequencing batch reactor containing municipal waste water treatment facility activated sludge performing simultaneous organic carbon oxidation and nitrification. The ability of activated sludge to remove propylene glycol was evaluated by studying the biodegradation and sorption characteristics of propylene glycol in an activated sludge medium. The results indicate sorption may play a role in the fate of propylene glycol in AS, and the heterotrophic bacteria readily degrade this compound. Therefore, a field deployable bioreactor may be appropriate for use in flight line applications.
Coincidence problems for generalized contractions
Authors: Falset Jesús , Mleşniţe Oana
Published: October 2013

In this paper, we establish some new existence, uniqueness and Ulam-Hyers
stability theorems for coincidence problems for two single-valued mappings.
The main results of this paper extend the results presented in O.
Mle?ni?e: Existence and Ulam-Hyers stability results for coincidence
problems, J. Non-linear Sci. Appl., 6(2013), 108-116. In the last section
two examples of application of these results are also given.
Selections and countable compactness
Authors: Buhagiar David , Gutev Valentin
Published: October 2013

The present paper deals with continuous extreme-like selections for the Vietoris hyperspace of countably compact spaces. Several new results and applications are established, along with some known results which are obtained under minimal hypotheses. The paper contains also a number of examples clarifying the role of countable compactness.
Measurement of mechanical properties of snow for simulation of skiing
Authors: Mössner Martin , Innerhofer Gerhard , Schindelwig Kurt , Kaps Peter , Schretter Herwig , Nachbauer Werner
Published: October 2013

In the simulation of skiing the force between ski and snow is a decisive factor. We decompose the reaction force into a penetration force normal to the snow surface, a shear force and friction. Two portable measurement devices were developed to study the penetration and shear forces for compacted snow on groomed ski slopes. The penetration force was assessed by measuring the penetration depth of a ski-tool loaded normal to the snow surface. For the shear force the tangential load was measured when the snow began to fail. Overall 236 penetration and 108 shear experiments were conducted on different types of snow. The penetration force was proportional to the volume of snow displaced by the ski-tool. The failure shear force was proportional to the penetration depth multiplied by the length of the tool. The constants of proportionality, HV and Sf, are material parameters of snow. The snow hardness, HV, varied between 0.04 and 90 N mm–3 and the failure shear stress, Sf, between 0.04 and 0.40 N mm–2. In another investigation, skiing turns were simulated using the presented snow reaction forces. Maximum deviations between computed and real trajectories were <1 % of the overall length of the runs.
Spatial and seasonal effects of temperature variability in a positive degree-day glacier surface mass-balance model
Authors: Seguinot Julien
Published: October 2013

The positive degree-day model is a parameterization of surface melt widely used for its simplicity (Hock, 2003).
Synthesis and Biological Activity of Diastereomeric and Geometric Analogs of Calcipotriol, PRI-2202 and PRI-2205, Against Human HL-60 Leukemia and MCF-7 Breast Cancer Cells
Authors: Milczarek Magdalena , Chodyński Michał , Filip-Psurska Beata , Martowicz Agnieszka , Krupa Małgorzata , Krajewski Krzysztof , Kutner Andrzej , Wietrzyk Joanna
Published: October 2013

Diastereomeric and geometric analogs of calcipotriol, PRI-2202 and PRI-2205, were synthesized as advanced intermediates from vitamin D C-22 benzothiazoyl sulfones and side-chain aldehydes using our convergent strategy. Calcitriol, calcipotriol (PRI-2201) and tacalcitol (PRI-2191) were used as the reference compounds. Among a series of tested analogs the diastereomeric analog PRI-2202 showed the strongest antiproliferative activity on the human breast cancer cell line MCF-7, whereas the geometric analog PRI-2205 was the weakest. Both analogs were less potent in antiproliferative activity against HL-60 cells compared to the reference compounds. The ability to potentiate antiproliferative effect of cisplatin or doxorubicin against HL-60 cells or that of tamoxifen against the MCF-7 cell line was observed at higher doses of PRI-2202 or PRI-2205 than those of the reference compounds. The proapoptotic activity of tamoxifen, expressed as the diminished mitochondrial membrane potential, as well as the increased phosphatidylserine expression, was partially attenuated by calcitriol, PRI-2191, PRI-2201 and PRI-2205. The treatment of the MCF-7 cells with tamoxifen alone resulted in an increase in VDR expression. Moreover, a further increase in VDR expression was observed when the analogs PRI-2201 or PRI-2205, but not PRI-2191, were used in combination with tamoxifen. This observation could partially explain the potentiation of the antiproliferative effect of tamoxifen by vitamin D analogs.
Optimal Thermal Design of a Stacked Mini-Channel Heat Sink Cooled by a Low Flow Rate Coolant
Authors: Pang Liping , Wang Minxing , Wang Wei , Liu Meng , Wang Jun
Published: October 2013

Application requirements for avionics are often very strict. For example, the heat sinks of avionics need very good temperature uniformity, but the flow rate of coolant is very restricted. In addition, the use of micro-channels is not recommended due to the potential clogging issue. Considering these design requirements, we will discuss a multiple-objective optimal design method to obtain a good stacked mini-channel structure for avionics applications. In our thermal design, the design variables are the mini-channel geometry parameters. Temperature uniformity, entropy generation, max temperature of heat sink and pump work are chosen as the objective functions. A Multi Objective Genetic Algorithm (MOGA) and Fluent solver are used together to minimize multiple objective functions subject to constraints, and locate the Pareto front. By analyzing the multiple objective optimal results, we can draw the conclusion that the objective functions of Tmax and sg have same effect on the optimization, and the multiple optimal results are a set and not a single value. If mostly focusing on the temperature uniformity, we can recommend some optimal structures to design a stacked mini-channel heat sink.
Sending Safety Video over WiMAX in Vehicle Communications
Authors: Huang Jun , Yang Oliver , Lawal Funmilyo
Published: October 2013

This paper reports on the design of an OPNET simulation platform to test the performance of sending real-time safety video over VANET (Vehicular Adhoc NETwork) using the WiMAX technology. To provide a more realistic environment for streaming real-time video, a video model was created based on the study of video traffic traces captured from a realistic vehicular camera, and different design considerations were taken into account. A practical controller over real-time streaming protocol is implemented to control data traffic congestion for future road safety development. Our driving video model was then integrated with the WiMAX OPNET model along with a mobility model based on real road maps. Using this simulation platform, different mobility cases have been studied and the performance evaluated in terms of end-to-end delay, jitter and visual experience.