bluuebunny/crossref_metadata_2025 huggingface dataset:
Impacts of Mineral Dust on Trace Element Concentrations (As, Cd, Cu, Ni and Pb) in Lichens and Soils at Lhù’ààn Mân’ (Yukon Territory, Canada)
Authors: Pouillé Sophie , Talbot Julie , Tamalavage Anne E. , Kessler‐Nadeau Max Émile , King James
Published: May 2024

Dust is a mineral aerosol of the atmosphere that often contains trace elements such as As, Cd, and Pb. Lhù’ààn Mân’ (Kluane Lake), located in southwestern Yukon, is a region of frequent dust activity. In 2016, the lake level fell due to a dramatic decrease in inflow from glacier meltwater, and the delta of the lake became an important source of dust to surrounding ecosystems. To determine the impacts of dust deposition on vegetation and soil trace element concentrations and characteristics, we sampled the lichen Peltigera canina and soil layers at 57 sites along a deposition gradient located 1.4–33.6 km downwind from the principal dust source. Arsenic, Cd, Cu, Ni and Pb in lichens were negatively correlated with the distance away from the dust source, with the highest correlations in Ni and Pb (r2 = 0.50 and 0.48, respectively). Lichen and tree abundances were negatively impacted by dust deposition, suggesting that dust can affect ecosystem vegetation composition. Starting 8 km away from the dust source, the concentrations of As, Ni, and Pb decreased by more than 50% per km, while Cd and Cu concentrations decreased by more than 40% per km. Overall, within the sampled ecosystems, soil pH is 1.4 times higher in the first 8 km from the dust source while carbon content and nutrients are lower, which implies changes in nutrient availability and cycling in dust‐affected ecosystems.
Carbon Cycle Response to Stratospheric Aerosol Injection With Multiple Temperature Stabilization Targets and Strategies
Authors: Zhao Mengying , Cao Long , Visioni Daniele , MacMartin Douglas G.
Published: May 2024

We analyze the global carbon cycle response to a set of stratospheric aerosol injection (SAI) simulations performed by the CESM2(WACCM6‐MA) model. The simulations are performed under the specified SSP2‐4.5 CO2 concentration pathway. It is found that both the temperature stabilization target and the SO2 injection strategy have important effects on the global carbon sink. Relative to the SSP2‐4.5 scenario, averaged over the last 20 years of our simulations (year 2050–2069), simultaneous multi‐location SO2 injection causes an increase in cumulative land carbon uptake of 45 and 23 PgC, and an increase in cumulative ocean carbon uptake of 6 and 2 PgC for temperature stabilization targets of 0.5°C and 1.5°C respectively. For a temperature stabilization target of 1.0°C, SO2 injections increase land and ocean carbon sinks by 22–42 PgC and 4–7 PgC, respectively, depending on the strategies of SO2 injections (low latitude, mid‐to‐high latitude, and multi‐objective injection). Relative to SSP2‐4.5, by year 2069, SAI increases diagnosed cumulative CO2 emissions by 25–53 PgC (3%–6%), implying a decrease in atmospheric CO2 if SO2 injections were performed under a prescribed CO2 emission pathway. Stratospheric SO2 injections slow permafrost thaw, but do not restore permafrost to the previous extent at the same warming level for all injection strategies. An abrupt termination of SO2 injection weakens both the ocean and land carbon sink, and causes a rapid decline of permafrost extent. A gradual phaseout of SO2 injection slows sharp decline of permafrost and delays the rebound of carbon sink.
Effects of vitamin D in pregnancy on maternal and offspring health-related outcomes: An umbrella review of systematic review and meta-analyses
Authors: Chien Mei-Chun , Huang Chueh-Yi , Wang Jie-Huei , Shih Chia-Lung , Wu Pensee
Published: May 2024

Background

Vitamin D deficiency has been linked with several adverse maternal and fetal outcomes.

Objective

To summarize systematic reviews and meta-analyses evaluating the effects of vitamin D deficiency and of vitamin D supplementation in pregnancy on maternal and offspring health-related outcomes.

Methods

Prior to conducting this umbrella review, we registered the protocol in PROSPERO (CRD42022368003). We conducted searches in PubMed, Embase, and Cochrane Library for systematic reviews and meta-analyses on vitamin D in pregnancy, from database inception to October 2, 2023. All outcomes related to vitamin D in pregnancy obtained from the systematic reviews and meta-analyses were extracted. Data Extraction: Two reviewers independently chose studies and collected information on health outcomes. The quality of the included articles’ methodology was assessed using AMSTAR 2 (A Measurement Tool to Assess Systematic Reviews–2).

Results

We identified 16 eligible systematic reviews and meta-analyses, which included 250,569 women. Our results demonstrated that vitamin D deficiency in pregnancy is associated with increased risk of preterm birth, small-for gestational age/low birth weight infants, recurrent miscarriage, bacterial vaginosis and gestational diabetes mellitus. Vitamin D supplementation in pregnancy increases birth weight, and reduces the risk of maternal pre-eclampsia, miscarriage, and vitamin D deficiency, fetal or neonatal mortality, as well as attention-deficit hyperactivity disorder, and autism spectrum disorder in childhood. In women with gestational diabetes mellitus, vitamin D supplementation in pregnancy can reduce the risk of maternal hyperbilirubinemia, polyhydramnios, macrosomia, fetal distress, and neonatal hospitalization.

Conclusion

Due to the association with adverse maternal and offspring health outcomes, we recommend the vitamin D status in pregnancy should be monitored, particularly in women at high risk of vitamin D deficiency. It is suggested that pregnant women take a dose of >400 IU/day of vitamin D supplementation during pregnancy to prevent certain adverse outcomes.
Evaluating the efficiency of two different over-the-counter tooth whitening systems: a randomised controlled clinical trial
Authors: Krayem Eenass , Banerjee Avijit , Milly Hussam
Published: May 2024

Objectives

To compare whitening efficiency and tooth sensitivity (TS) of two different over the counter (OTC) whitening systems in comparison to conventional at-home bleaching using 20% carbamide peroxide.

Materials and methods

A randomised controlled clinical trial was conducted with three parallel groups (n = 13): (A) at-home whitening using 20% carbamide peroxide (20% CP), (B) OTC ready-to-use gel trays and (C) OTC-paint on gel. Clinical colour change values (ΔE) were measured using spectrophotometry at T0: baseline, T1: 7-day and T2: 14-day from whitening start, T3: 2 weeks and T4: 6 months after whitening end. TS was recorded using a visual analogue scale (VAS). ΔE and TS values were statistically analysed. The level of significance for all tests was 5%.

Results

Significant differences in ∆E values were recorded between the experimental groups. ∆E values were significantly higher in the 20% CP conventional at-home whitening group. TS measurements were significantly higher in the 20% CP whitening group (p < 0.05).

Conclusion

Conventional at-home whitening revealed significantly improved colour change when compared to the OTC-paint on gel and OTC ready-to-use gel tray whitening systems. There was a significant colour relapse in OTC systems.

Clinical relevance

The use of tested OTC systems is not recommended as they are not effective in a clear and prolonged improved shade change. Conventional at-home whitening using 20% CP showed higher whitening efficiency and colour stability. This trial was registered with a International Standard Randomised Controlled Trial Number (ISRCTN23096480), Registration date: 12/04/2023.
Optimizing a self-solidifying hydrogel as an endoscopically deliverable hydrogel coating system: a proof-of-concept study on porcine endoscopic submucosal dissection-induced ulcers
Authors: Cho Rina , Kamata Hiroyuki , Tsuji Yosuke , Fujisawa Ayano , Miura Yuko , Ishikawa Shohei , Sato Ren , Katashima Takuya , Sakai Takamasa , Fujishiro Mitsuhiro
Published: May 2024

Endoscopic submucosal dissection (ESD) benefits patients in the early stages of cancer, but it poses various risks of complication. Strategies involving the application of clinically approved products to cover ulcers caused by ESD can reduce these complications, but the fixed nature of their properties limit the understanding of their effects on ulcer healing. This study was focused on Tetra–PEG gel, an innovative hydrogel with controllable physical properties made from a sulfhydryl–maleimide pair. The use of biocompatible polyethylene glycol (PEG) in Tetra–PEG gel may allow for its application as a biomaterial. The aims of our study were to identify the characteristics of a self-solidifying hydrogel for endoscopic application and to develop a new ulcer coating agent for post-ESD treatment. We developed a specialized double-lumen catheter and determined the optimal application conditions of the hydrogel. We examined the hydrodynamic properties of the gelling solutions and elucidated the pressure drop that occurred during device operation. Finally, by considering previous experimental results, we successfully applied the hydrogel to post-ESD ulcers in porcine stomachs. We believed that by further optimizing hydrogels with effectively controlled properties and by continuing to investigate them through animal experiments, we could expand our understanding of the relationships among material and ulcer healing properties and apply this knowledge to clinical applications.
Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint
Authors: Naschberger Andreas , Fadeeva Mariia , Klaiman Daniel , Borovikova-Sheinker Anna , Caspy Ido , Nelson Nathan , Amunts Alexey
Published: May 2024

Plant photosystem I (PSI) consists of at least 13 nuclear-encoded and 4 chloroplast-encoded subunits that together act as a sunlight-driven oxidoreductase. Here we report the structure of a PSI assembly intermediate that we isolated from greening oat seedlings. The assembly intermediate shows an absence of at least eight subunits, including PsaF and LHCI, and lacks photoreduction activity. The data show that PsaF is a regulatory checkpoint that promotes the assembly of LHCI, effectively coupling biogenesis to function.
Genome resources for three modern cotton lines guide future breeding efforts
Authors: Sreedasyam Avinash , Lovell John T. , Mamidi Sujan , Khanal Sameer , Jenkins Jerry W. , Plott Christopher , Bryan Kempton B. , Li Zhigang , Shu Shengqiang , Carlson Joseph , Goodstein David , De Santiago Luis , Kirkbride Ryan C. , Calleja Sebastian , Campbell Todd , Koebernick Jenny C. , Dever Jane K. , Scheffler Jodi A. , Pauli Duke , Jenkins Johnie N. , McCarty Jack C. , Williams Melissa , Boston LoriBeth , Webber Jenell , Udall Joshua A. , Chen Z. Jeffrey , Bourland Fred , Stiller Warwick N. , Saski Christopher A. , Grimwood Jane , Chee Peng W. , Jones Don C. , Schmutz Jeremy
Published: May 2024

Cotton (Gossypium hirsutum L.) is the key renewable fibre crop worldwide, yet its yield and fibre quality show high variability due to genotype-specific traits and complex interactions among cultivars, management practices and environmental factors. Modern breeding practices may limit future yield gains due to a narrow founding gene pool. Precision breeding and biotechnological approaches offer potential solutions, contingent on accurate cultivar-specific data. Here we address this need by generating high-quality reference genomes for three modern cotton cultivars (‘UGA230’, ‘UA48’ and ‘CSX8308’) and updating the ‘TM-1’ cotton genetic standard reference. Despite hypothesized genetic uniformity, considerable sequence and structural variation was observed among the four genomes, which overlap with ancient and ongoing genomic introgressions from ‘Pima’ cotton, gene regulatory mechanisms and phenotypic trait divergence. Differentially expressed genes across fibre development correlate with fibre production, potentially contributing to the distinctive fibre quality traits observed in modern cotton cultivars. These genomes and comparative analyses provide a valuable foundation for future genetic endeavours to enhance global cotton yield and sustainability.
Flexible yet impermeable composites with wrinkle structured BNNSs assembling for high-performance thermal management
Authors: Guo Guilei , Liu Yijie , Ding Yafei , Liu Wenjie , Zhu Guimei , Hao Xiaoli , Huang Xingyi , Xia Jianfei , Li Baowen , Zhang Tong-Yi , Sun Bin
Published: May 2024

Efficient thermal management has become one of the most critical issues of electronics because of the high heat flux generated from highly integrated, miniaturized, and increased power. Here we report highly flexible composites with aligned and overlapping interconnected boron nitride nanosheets (BNNSs) assembled in wrinkle structures. Besides high in-plane thermal conductivity of more than 26.58 W m−1 K−1, such structure rendered enhanced through-plane conduction along with increasing pre-stain. As thermal interface materials (TIMs) of both rigid and flexible devices, the composites revealed an outstanding thermal cooling capability outperforming some commercial TIMs. During a record-long bending process of more than 3000 cycles, the maximum temperature fluctuation of the flexible device with 100%-prestrained composite was only within 0.9 °C, less than one-third of that with commercial thermal pad. Moreover, the composite revealed a superior impermeability for flexible seals. Our results illustrate that the composites could be an ideal candidate for the thermal management of emerging flexible electronics.
Coupling conduction-band valleys in SiGe heterostructures via shear strain and Ge concentration oscillations
Authors: Woods Benjamin D. , Soomro Hudaiba , Joseph E. S. , Frink Collin C. D. , Joynt Robert , Eriksson M. A. , Friesen Mark
Published: May 2024

Engineering conduction-band valley couplings is a key challenge for Si-based spin qubits. Recent work has shown that the most reliable method for enhancing valley couplings entails adding Ge concentration oscillations to the quantum well. However, ultrashort oscillation periods are difficult to grow, while long oscillation periods do not provide useful improvements. Here, we show that the main benefits of short-wavelength oscillations can be achieved in long-wavelength structures through a second-order coupling process involving Brillouin-zone folding induced by shear strain. We finally show that such strain can be achieved through common fabrication techniques, making this an exceptionally promising system for scalable quantum computing.
Detection of atmospheric radon concentration anomalies and their potential for earthquake prediction using Random Forest analysis
Authors: Tsuchiya Mayu , Nagahama Hiroyuki , Muto Jun , Hirano Mitsuhiro , Yasuoka Yumi
Published: May 2024

Various anomalies occurring before earthquakes are currently being studied to predict seismic events, with one of them being the radioactive element radon (222Rn). Radon concentrations in the soil, water, and atmosphere fluctuate in response to crustal movement. Recent research has statistically detected anomalies by analyzing the fluctuations in radon concentrations before earthquakes and conducting quantitative evaluations of radon. However, the method used to determine the parameters in the analysis was problematic. Therefore, in this study, we compared observed atmospheric radon concentration data with predicted values based on typical annual patterns using Random Forest analysis. We conducted a more objective analysis by employing this method and statistically determining anomalies using thresholds. This analysis was conducted using atmospheric radon concentration observation data obtained at Kobe Pharmaceutical University (KPU) before the 1995 Kobe Earthquake, and ionization currents emitted when radon decays were obtained at Fukushima Medical University (FMU) before the 2011 Tohoku-oki Earthquake. Consequently, before the major earthquakes occurred at both locations, the difference between the predicted and observed values exceeded the standard deviation by a factor of three. These results indicate the potential of Random Forest analysis to identify anomalies in atmospheric radon concentrations before earthquakes occur.
Robust digital-twin airspace discretization and trajectory optimization for autonomous unmanned aerial vehicles
Authors: ElSayed Mo , Mohamed Moataz
Published: May 2024

The infiltration of heterogenous fleets of autonomous Unmanned Aerial Vehicles (UAVs) in smart cities is leading to the consumerization of city air space which includes infrastructure creation of roads, traffic design, capacity estimation, and trajectory optimization. This study proposes a novel autonomous Advanced Aerial Mobility (AAM) logistical system for high density city centers. First, we propose a real-time 3D geospatial mining framework for LiDAR data to create a dynamically updated digital twin model. This enables the identification of viable airspace volumes in densely populated 3D environments based on the airspace policy/regulations. Second, we propose a robust city airspace dynamic 4D discretization method (Skyroutes) for autonomous UAVs to incorporate the underlying real-time constraints coupled with externalities, legal, and optimal UAV operation based on kinematics. An hourly trip generation model was applied to create 1138 trips in two scenarios comparing the cartesian discretization to our proposed algorithm. The results show that the AAM enables a precise airspace capacity/cost estimation, due to its detailed 3D generation capabilities. The AAM increased the airspace capacity by up to 10%, the generated UAV trajectories are 50% more energy efficient, and significantly safer.
Prognostic subgroups of chronic pain patients using latent variable mixture modeling within a supervised machine learning framework
Authors: Zhao Xiang , Dannenberg Katharina , Repsilber Dirk , Gerdle Björn , Molander Peter , Hesser Hugo
Published: May 2024

The present study combined a supervised machine learning framework with an unsupervised method, finite mixture modeling, to identify prognostically meaningful subgroups of diverse chronic pain patients undergoing interdisciplinary treatment. Questionnaire data collected at pre-treatment and 1-year follow up from 11,995 patients from the Swedish Quality Registry for Pain Rehabilitation were used. Indicators measuring pain characteristics, psychological aspects, and social functioning and general health status were used to form subgroups, and pain interference at follow-up was used for the selection and the performance evaluation of models. A nested cross-validation procedure was used for determining the number of classes (inner cross-validation) and the prediction accuracy of the selected model among unseen cases (outer cross-validation). A four-class solution was identified as the optimal model. Identified subgroups were separable on indicators, predictive of long-term outcomes, and related to background characteristics. Results are discussed in relation to previous clustering attempts of patients with diverse chronic pain conditions. Our analytical approach, as the first to combine mixture modeling with supervised, targeted learning, provides a promising framework that can be further extended and optimized for improving accurate prognosis in pain treatment and identifying clinically meaningful subgroups among chronic pain patients.
Efficacy and safety of stem cell transplantation for multiple sclerosis: a systematic review and meta-analysis of randomized controlled trials
Authors: Nawar Asmaa Ahmed , Farid Aml Mostafa , Wally Rim , Tharwat Engy K. , Sameh Ahmed , Elkaramany Yomna , Asla Moamen Mostafa , Kamel Walaa A.
Published: May 2024

Multiple sclerosis (MS) is a common autoimmune neurological disease affecting patients’ motor, sensory, and visual performance. Stem Cell Transplantation (SCT) is a medical intervention where a patient is infused with healthy stem cells with the purpose of resetting their immune system. SCT shows remyelinating and immunomodulatory functions in MS patients, representing a potential therapeutic option. We conducted this systematic review and meta-analysis that included randomized control trials (RCTs) of SCT in MS patients to investigate its clinical efficacy and safety, excluding observational and non-English studies. After systematically searching PubMed, Web of Science, Scopus, and Cochrane Library until January 7, 2024, nine RCTs, including 422 patients, were eligible. We assessed the risk of bias (ROB) in these RCTs using Cochrane ROB Tool 1. Data were synthesized using Review Manager version 5.4 and OpenMeta Analyst software. We also conducted subgroup and sensitivity analyses. SCT significantly improved patients expanded disability status scale after 2 months (N = 39, MD =  − 0.57, 95% CI [− 1.08, − 0.06], p = 0.03). SCT also reduced brain lesion volume (N = 136, MD = − 7.05, 95% CI [− 10.69, − 3.4], p = 0.0002). The effect on EDSS at 6 and 12 months, timed 25-foot walk (T25-FW), and brain lesions number was nonsignificant. Significant adverse events (AEs) included local reactions at MSCs infusion site (N = 25, RR = 2.55, 95% CI [1.08, 6.03], p = 0.034) and hematological disorders in patients received immunosuppression and autologous hematopoietic SCT (AHSCT) (N = 16, RR = 2.33, 95% CI [1.23, 4.39], p = 0.009). SCT can improve the disability of MS patients and reduce their brain lesion volume. The transplantation was generally safe and tolerated, with no mortality or significant serious AEs, except for infusion site reactions after mesenchymal SCT and hematological AEs after AHSCT. However, generalizing our results is limited by the sparse number of RCTs conducted on AHSCT. Our protocol was registered on PROSPERO with a registration number: CRD42022324141.
Decoding intelligence via symmetry and asymmetry
Authors: Fu Jianjing , Hsiao Ching-an
Published: May 2024

Humans use pictures to model the world. The structure of a picture maps to mind space to form a concept. When an internal structure matches the corresponding external structure, an observation functions. Whether effective or not, the observation is self-consistent. In epistemology, people often differ from each other in terms of whether a concept is probabilistic or certain. Based on the effect of the presented IG and pull anti algorithm, we attempt to provide a comprehensive answer to this problem. Using the characters of hidden structures, we explain the difference between the macro and micro levels and the same difference between semantics and probability. In addition, the importance of attention is highlighted through the combination of symmetry and asymmetry included and the mechanism of chaos and collapse revealed in the presented model. Because the subject is involved in the expression of the object, representationalism is not complete. However, people undoubtedly reach a consensus based on the objectivity of the representation. Finally, we suggest that emotions could be used to regulate cognition.
Bio-synthesis, purification and structural analysis of Cyclosporine-A produced by Tolypocladium inflatum with valorization of agro-industrial wastes
Authors: Falah Fereshteh , Samie Ali , Mortazavi Seyed Ali , Danesh Abolghasem , Yazdi Farideh Tabatabaei , Ramezani Mohammad
Published: May 2024

Cyclosporine A (CyA) holds significant importance as a strategic immunosuppressive drug for organ transplant patients. In this study, we aimed to produce pure and cost-effective Cyclosporine A (CyA) by fermenting a culture medium containing dairy sludge, using Tolypocladium inflatum PTCC 5253. Following the fermentation stage, ethyl acetate extraction and fast protein liquid chromatography were employed for sample purification. The initial evaluation of the effectiveness of CyA obtained from these processes was performed through bioassay, wherein the antimicrobial clear zone diameter was found to be larger compared to the sample obtained from the fermentation culture. The concentration of CyA was determined using high-performance liquid chromatography, yielding values of 334 mg/L, 456 mg/L, and 578 mg/L for the fermented, extracted, and purified samples, respectively. Further analysis utilizing liquid chromatography tandem mass spectrometry (LC/MS/MS) confirmed a purity of 91.9% and proper agreement with the standard sample based on the ion intensity of Z/m 1205. To validate the structure of CyA, nuclear magnetic resonance spectroscopy, Fourier-transform infrared (FT-IR), and Raman spectroscopy were employed. X-ray diffraction and differential scanning calorimetry analyses demonstrated that the purified CyA exhibited a crystal structure similar to the standard sample, characterized by two broad peaks at 2θ = 9° and 20°, and comparable glass transition temperatures (57–68 °C for the purified sample; 53–64 °C for the standard sample). Dynamic light scattering analysis confirmed a uniform particle size distribution in both the purified and standard samples. The zeta potentials of the purified and standard samples were determined to be − 25.8 ± 0.16 and − 23.63 ± 0.12 mV, respectively. Our results demonstrate that dairy sludge can serve as a suitable culture medium for the production of (CyA).
Research on provincial water resources carrying capacity and coordinated development in China based on combined weighting TOPSIS model
Authors: Zhu Qianying , Cao Yi
Published: May 2024

With the continuous development of the economy and society, along with the sustained population growth, the issue of water resources carrying capacity in China has attracted increasing attention. This paper constructs a model for evaluating the provincial water resources carrying capacity in China from four dimensions: water, economy, society, and ecology. Utilizing this model, we analyze the spatiotemporal variations in water resources carrying capacity among 31 provinces in China from 2005 to 2021. Additionally, we delve into the coupling coordination and influencing factors of water resources carrying capacity. The study reveals an overall increasing trend in China’s water resources carrying capacity index, with the ecological indicator exhibiting the most significant growth while the water resources sub-indicator lags behind. There are notable regional differences, with higher water resources carrying capacity observed in the eastern coastal areas and relatively lower capacity in the western regions. The ecological criterion becomes a core factor constraining water resources carrying capacity from 2005 to 2015, gradually giving way to the prominence of the social criterion since 2015. The coordination degree is relatively higher in the eastern regions, more scattered in the western regions, and relatively stable in the central regions. Based on the research findings, a series of recommendations are proposed, including strengthening environmental protection policies, optimizing water resources management mechanisms, improving water use efficiency, and promoting economic structural diversification. These suggestions aim to facilitate the sustainable development of water resources carrying capacity in China.
Pulsed electric field induces exocytosis and overexpression of MAGE antigens in melanoma
Authors: Szlasa Wojciech , Sauer Natalia , Baczyńska Dagmara , Ziętek Marcin , Haczkiewicz-Leśniak Katarzyna , Karpiński Paweł , Fleszar Mariusz , Fortuna Paulina , Kulus Michał J. , Piotrowska Aleksandra , Kmiecik Alicja , Barańska Agnieszka , Michel Olga , Novickij Vitalij , Tarek Mounir , Kasperkiewicz Paulina , Dzięgiel Piotr , Podhorska-Okołów Marzenna , Saczko Jolanta , Kulbacka Julita
Published: May 2024

Nanosecond pulsed electric field (nsPEF) has emerged as a promising approach for inducing cell death in melanoma, either as a standalone treatment or in combination with chemotherapeutics. However, to date, there has been a shortage of studies exploring the impact of nsPEF on the expression of cancer-specific molecules. In this investigation, we sought to assess the effects of nsPEF on melanoma-specific MAGE (Melanoma Antigen Gene Protein Family) expression. To achieve this, melanoma cells were exposed to nsPEF with parameters set at 8 kV/cm, 200 ns duration, 100 pulses, and a frequency of 10 kHz. We also aimed to comprehensively describe the consequences of this electric field on melanoma cells' invasion and proliferation potential. Our findings reveal that following exposure to nsPEF, melanoma cells release microvesicles containing MAGE antigens, leading to a simultaneous increase in the expression and mRNA content of membrane-associated antigens such as MAGE-A1. Notably, we observed an unexpected increase in the expression of PD-1 as well. While we did not observe significant differences in the cells' proliferation or invasion potential, a remarkable alteration in the cells' metabolomic and lipidomic profiles towards a less aggressive phenotype was evident. Furthermore, we validated these results using ex vivo tissue cultures and 3D melanoma culture models. Our study demonstrates that nsPEF can elevate the expression of membrane-associated proteins, including melanoma-specific antigens. The mechanism underlying the overexpression of MAGE antigens involves the initial release of microvesicles containing MAGE antigens, followed by a gradual increase in mRNA levels, ultimately resulting in elevated expression of MAGE antigens post-experiment. These findings shed light on a novel method for modulating cancer cells to overexpress cancer-specific molecules, thereby potentially enhancing their sensitivity to targeted anticancer therapy.
Flow regimes identification of air water counter current flow in vertical annulus using differential pressure signals and machine learning
Authors: Cao Feng , Dang Ruirong , Dang Bo , Zheng Huifeng , Ji Anzhao , Chen Zhanjun , Zhao Jiaxuan , Sun Zhimeng
Published: May 2024

This study investigates the gas–liquid two-phase counter-current flow through a vertical annulus, a phenomenon prevalent across numerous industrial fields. The presence of an inner pipe and varying degrees of eccentricity between the inner and outer pipes often blur the clear demarcation of flow regime boundaries. To address this, we designed a vertical annulus with adjustable eccentricity (outer and inner diameters of 125 mm and 75 mm, respectively). We conducted gas–liquid counter-current flow experiments under specific conditions: gas superficial velocity ranging from 0.06 to 5.04 m/s, liquid superficial velocity from 0.01 to 0.25 m/s, and five levels of eccentricity (e = 0, 0.25, 0.5, 0.75, 1). We collected differential pressure data at two distinct height distances (DP1: 50 mm and DP2: 1000 mm). We used vectors, composed of both the probability density functions (PDFs) of the differential pressure signals and the power spectral density (PSD) reduced via Principal Component Analysis, as features. Using the CFDP clustering algorithm—based on local density—we clustered the flow regimes of the experimental data, thereby achieving an objective and consistent identification of the flow regime of gas–liquid two-phase counter-current flow in a vertical annulus. Our analysis reveals that for DP1, the main differences in the PSD of various flow regimes occur within the 0.5–1 Hz range. Among the three flow regimes involved, the slug flow exhibits the highest power intensity, followed by the bubbly flow, with the churn flow having the least. In terms of differential pressure distribution, the bubbly and churn flows have a concentrated distribution, while the slug flow is more dispersed. For DP2, the PSD differences primarily exist within the 0.5–2 Hz range. The churn flow has the highest power intensity, followed by the slug flow, with the bubbly flow being the weakest. Here, the bubbly flow's differential pressure distribution is concentrated, while the slug and churn flows are more dispersed. Based on the results of the flow regime classification, we generated a flow regime map and analyzed the influence of annulus eccentricity on the flow regime. We found that in most cases, pipe eccentricity does not significantly affect the flow regime. However, in the transition region—such as the bubbly to slug flow transition zone—flows with medium eccentricity values (e = 0.5, 0.75) are more likely to transition to slug flow. We compared the visual recognition results of flow regimes with the clustering results. 4.04% of the total samples showed different results from visual recognition and clustering, primarily located in the flow regime transition area. Since visually distinguishing flow regimes in these areas is typically challenging, our methodology offers an objective classification approach for gas–liquid two-phase counter-current flow in a vertical annulus.
Blood perfusion with polymyxin B immobilized columns in patients with COVID-19 requiring oxygen therapy
Authors: Katagiri Daisuke , Tsukada Akinari , Izumi Shinyu , Shimizu Yosuke , Terada-Hirashima Junko , Uemura Yukari , Kusaba Yusaku , Takasaki Jin , Takoi Hiroyuki , Tamura-Nakano Miwa , Hojo Masayuki , Takano Hideki , Noiri Eisei , Abe Shinji , Azuma Arata , Sugiyama Haruhito
Published: May 2024

Extracorporeal blood purification with polymyxin B immobilized fiber column direct hemoperfusion (PMX-DHP), is reported to be effective in treating COVID-19 pneumonitis with oxygen demand. This multicenter prospective study evaluated the efficacy and safety of PMX-DHP in oxygen-requiring patients with COVID-19 admitted between September 28, 2020, and March 31, 2022. The primary endpoint was the percentage of clinical improvement 15 days after treatment. The secondary endpoint was the percentage of worsened disease status. Data from the COVID-19 patient registry were used for the synthetic control group. The improvement rate on Day 15 did not differ between PMX-treated patients and controls; however, the deterioration rate was 0.38 times lower in the PMX-treated group, and the death rates on Day 29 were 0 and 11.1% in the PMX-treated and control groups, respectively. The PMX group showed a 0.73 times higher likelihood for reduced intensive care demand, as 16.7% of PMX-treated patients and 22.8% of controls worsened. After treatment blood oxygenation improved, urinary β2-microglobulin and liver-type fatty acid-binding protein showed significant decreases, and IL-6 decreased once during treatment but did not persist. In this study, PMX treatment effectively prevented the worsening of COVID-19 pathology, accompanied by improved oxygenation. PMX treatment to remove activated cells may effectively improve patient outcomes.
Construction of Vero cell-adapted rabies vaccine strain by five amino acid substitutions in HEP-Flury strain
Authors: Harada Michiko , Matsuu Aya , Park Eun-Sil , Inoue Yusuke , Uda Akihiko , Kaku Yoshihiro , Okutani Akiko , Posadas-Herrera Guillermo , Ishijima Keita , Inoue Satoshi , Maeda Ken
Published: May 2024

Rabies virus (RABV) causes fatal neurological disease. Pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP) using inactivated-virus vaccines are the most effective measures to prevent rabies. In Japan, HEP-Flury, the viral strain, used as a human rabies vaccine, has historically been propagated in primary fibroblast cells derived from chicken embryos. In the present study, to reduce the cost and labor of vaccine production, we sought to adapt the original HEP-Flury (HEP) to Vero cells. HEP was repeatedly passaged in Vero cells to generate ten- (HEP-10V) and thirty-passaged (HEP-30V) strains. Both HEP-10V and HEP-30V grew significantly better than HEP in Vero cells, with virulence and antigenicity similar to HEP. Comparison of the complete genomes with HEP revealed three non-synonymous mutations in HEP-10V and four additional non-synonymous mutations in HEP-30V. Comparison among 18 recombinant HEP strains constructed by reverse genetics and vesicular stomatitis viruses pseudotyped with RABV glycoproteins indicated that the substitution P(L115H) in the phosphoprotein and G(S15R) in the glycoprotein improved viral propagation in HEP-10V, while in HEP-30V, G(V164E), G(L183P), and G(A286V) in the glycoprotein enhanced entry into Vero cells. The obtained recombinant RABV strain, rHEP-PG4 strain, with these five substitutions, is a strong candidate for production of human rabies vaccine.
Perception of experience influences altruism and perception of agency influences trust in human–machine interactions
Authors: Oudah Mayada , Makovi Kinga , Gray Kurt , Battu Balaraju , Rahwan Talal
Published: May 2024

As robots become increasingly integrated into social economic interactions, it becomes crucial to understand how people perceive a robot’s mind. It has been argued that minds are perceived along two dimensions: experience, i.e., the ability to feel, and agency, i.e., the ability to act and take responsibility for one’s actions. However, the influence of these perceived dimensions on human–machine interactions, particularly those involving altruism and trust, remains unknown. We hypothesize that the perception of experience influences altruism, while the perception of agency influences trust. To test these hypotheses, we pair participants with bot partners in a dictator game (to measure altruism) and a trust game (to measure trust) while varying the bots’ perceived experience and agency, either by manipulating the degree to which the bot resembles humans, or by manipulating the description of the bots’ ability to feel and exercise self-control. The results demonstrate that the money transferred in the dictator game is influenced by the perceived experience, while the money transferred in the trust game is influenced by the perceived agency, thereby confirming our hypotheses. More broadly, our findings support the specificity of the mind hypothesis: Perceptions of different dimensions of the mind lead to different kinds of social behavior.
Microbiome dysbiosis in patients with chronic endometritis and Clostridium tyrobutyricum ameliorates chronic endometritis in mice
Authors: Liu Jiujiu , Tang Xiaorong , Chen Lei , Zhang Yue , Gao Jinfang , Wang Aiming
Published: May 2024

Chronic endometritis is associated with the imbalance of female reproductive tract microbiota and pathogenic microbial infection. This study aimed to identify the specific changes in the endometrial microbiome in patients with endometritis and to explore how Clostridium tyrobutyricum (C.t) influences the progression of endometritis in mice for further elucidating endometritis pathogenesis. For this purpose, endometrial tissues from 100 participants were collected and divided into positive, weakly positive, and negative groups based on CD138 levels, while endometrial microbiome differences were detected and analyzed using 16S rRNA gene sequencing. Staphylococcus aureus (S. aureus)-induced endometritis mouse model was established, followed by treatment with C.t, and inflammatory response, epithelial barrier, and TLR4/NF-κB pathway were evaluated. Results showed that α- and β-diversity was significantly lower in the positive group compared with the weakly positive or negative groups, where the negative group had more unique operational taxonomic units. The abundance of Proteobacteria was found to be increased, while that of Actinobacteria, Firmicutes, and Bacteroidetes was found to be reduced in the positive group, while the area under the curve value was found to be 0.664. Furthermore, C.t treatment resulted in the alleviation of S. aureus-induced inflammatory response, epithelial barrier damage, and activation of the TLR4/NF-κB pathway in mice. Clinical samples analysis revealed that the diversity and abundance of microbiota were altered in patients with endometritis having positive CD138 levels, while mechanistic investigations revealed C.t alleviated S. aureus-induced endometritis by inactivating TLR4/NF-κB pathway. The findings of this study are envisaged to provide a diagnostic and therapeutic potential of microbiota in endometritis.
Laparoscopic distal gastrectomy skill evaluation from video: a new artificial intelligence-based instrument identification system
Authors: Matsumoto Shiro , Kawahira Hiroshi , Fukata Kyohei , Doi Yasunori , Kobayashi Nao , Hosoya Yoshinori , Sata Naohiro
Published: May 2024

The advent of Artificial Intelligence (AI)-based object detection technology has made identification of position coordinates of surgical instruments from videos possible. This study aimed to find kinematic differences by surgical skill level. An AI algorithm was developed to identify X and Y coordinates of surgical instrument tips accurately from video. Kinematic analysis including fluctuation analysis was performed on 18 laparoscopic distal gastrectomy videos from three expert and three novice surgeons (3 videos/surgeon, 11.6 h, 1,254,010 frames). Analysis showed the expert surgeon cohort moved more efficiently and regularly, with significantly less operation time and total travel distance. Instrument tip movement did not differ in velocity, acceleration, or jerk between skill levels. The evaluation index of fluctuation β was significantly higher in experts. ROC curve cutoff value at 1.4 determined sensitivity and specificity of 77.8% for experts and novices. Despite the small sample, this study suggests AI-based object detection with fluctuation analysis is promising because skill evaluation can be calculated in real time with potential for peri-operational evaluation.
Regulation of TSC2 lysosome translocation and mitochondrial turnover by TSC2 acetylation status
Authors: Marqués Patricia , Burillo Jesús , González-Blanco Carlos , Jiménez Beatriz , García Gema , García-Aguilar Ana , Iglesias-Fortes Sarai , Lockwood Ángela , Guillén Carlos
Published: May 2024

Sirtuin1 (SIRT1) activity decreases the tuberous sclerosis complex 2 (TSC2) lysine acetylation status, inhibiting the mechanistic target of rapamycin complex 1 (mTORC1) signalling and concomitantly, activating autophagy. This study analyzes the role of TSC2 acetylation levels in its translocation to the lysosome and the mitochondrial turnover in both mouse embryonic fibroblast (MEF) and in mouse insulinoma cells (MIN6) as a model of pancreatic β cells. Resveratrol (RESV), an activator of SIRT1 activity, promotes TSC2 deacetylation and its translocation to the lysosome, inhibiting mTORC1 activity. An improvement in mitochondrial turnover was also observed in cells treated with RESV, associated with an increase in the fissioned mitochondria, positive autophagic and mitophagic fluxes and an enhancement of mitochondrial biogenesis. This study proves that TSC2 in its deacetylated form is essential for regulating mTORC1 signalling and the maintenance of the mitochondrial quality control, which is involved in the homeostasis of pancreatic beta cells and prevents from several metabolic disorders such as Type 2 Diabetes Mellitus.
Complexities of peroxyacetyl nitrate photochemistry and its control strategies in contrasting environments in the Pearl River Delta region
Authors: Liu Tao , Wang Yu , Cai Huang , Wang Hao , Zhang Chunlin , Chen Jun , Dai Yan , Zhao Wenlong , Li Jiangyong , Gong Daocheng , Chen Duohong , Zhai Yuhong , Zhou Yan , Liao Tong , Wang Boguang
Published: May 2024

Peroxyacetyl nitrate (PAN) is a hazardous secondary pollutant and a nitrogen reservoir in the troposphere, while comprehensive studies on its spatiotemporal distribution, chemical sources, and control strategies are limited. This study addressed these issues based on 1-year multi-site observations in the Pearl River Delta region, showing a pattern of higher PAN levels in the cold season, particularly in coastal areas. Moreover, in situ modeling indicated a net photochemical PAN formation in the warm season, while PAN was dominated by regional transport in the cold season (i.e., rural and coastal areas), releasing NO2 and PA radicals and promoting ozone (O3) formation. In addition, the feasibility of joint control of PAN and O3 was confirmed by their isopleth diagrams. C7–C9 aromatics and C4–C5 alkenes, largely from vehicle exhaust and solvent usage, were identified as the predominant contributors to PAN formation in this region. Overall, this study deepens our understanding of PAN chemistry and provides valuable insights into its control measures.
African food system and biodiversity mainly affected by urbanization via dietary shifts
Authors: De Vos Koen , Janssens Charlotte , Jacobs Liesbet , Campforts Benjamin , Boere Esther , Kozicka Marta , Leclère David , Havlík Petr , Hemerijckx Lisa-Marie , Van Rompaey Anton , Maertens Miet , Govers Gerard
Published: May 2024

The rapid urbanization in Africa profoundly affects local food and ecological systems. According to earlier research, urbanization may cause food production and biodiversity losses as agricultural or natural lands are absorbed by expanding cities. Land-use displacement effects may buffer agricultural production losses or may lead to additional biodiversity losses but are often overlooked. Moreover, impacts of dietary changes associated with urbanization are rarely considered. To address this, we combined spatially explicit projections of African urban area expansion with observed rice consumption shifts to inform a partial equilibrium model (the Global Biosphere Management Model). We demonstrate the importance of displacement effects to identify potential food production or biodiversity issues until 2050 and argue for their integration in land-use planning and policymaking across spatial scales. We identify that because of agricultural displacement, the impact of urban area expansion on food production losses is probably limited (<1%)—at the cost of additional losses of natural lands by 2050 (up to 2 Mt). We also show that considering dietary shifts associated with urbanization increases rice consumption, production (+8.0%), trade (up to +2 Mt of required import) and agricultural methane emissions (up to +12 MtCO2-equivalent yr–1), thereby underscoring the need for a systems approach in future sustainability studies.
Decidual natural killer cells dysfunction is caused by IDO downregulation in dMDSCs with Toxoplasma gondii infection
Authors: Wang Yu , Zhao Xiaoyue , Li Zhidan , Wang Wenxiao , Jiang Yuzhu , Zhang Haixia , Liu Xianbing , Ren Yushan , Xu Xiaoyan , Hu Xuemei
Published: May 2024

Myeloid-derived suppressor cells (MDSCs) play a crucial role in maintaining maternal-fetal tolerance by expressing some immune-suppressive molecules, such as indoleamine 2,3-dioxygenase (IDO). Toxoplasma gondii (T. gondii) infection can break the immune microenvironment of maternal-fetal interface, resulting in adverse pregnancy outcomes. However, whether T. gondii affects IDO expression in dMDSCs and the molecular mechanism of its effect are still unclear. Here we show, the mRNA level of IDO is increased but the protein level decreased in infected dMDSCs. Mechanistically, the upregulation of transcriptional levels of IDO in dMDSCs is regulated through STAT3/p52-RelB pathway and the decrease of IDO expression is due to its degradation caused by increased SOCS3 after T. gondii infection. In vivo, the adverse pregnancy outcomes of IDO−/− infected mice are more severe than those of wide-type infected mice and obviously improved after exogenous kynurenine treatment. Also, the reduction of IDO in dMDSCs induced by T. gondii infection results in the downregulation of TGF-β and IL-10 expression in dNK cells regulated through Kyn/AhR/SP1 signal pathway, eventually leading to the dysfunction of dNK cells and contributing the occurrence of adverse pregnancy outcomes. This study reveals a novel molecular mechanism in adverse pregnancy outcome induced by T. gondii infection.