bluuebunny/crossref_metadata_2025 huggingface dataset:
Dissecting the metal resistance genes contributed by virome from mining-affected metal contaminated soils
Authors: Zhang Hetian , Huang Jingyi , Zeng Weiai , Xiao Qinzhi , Zhu Yi , Kong Wuyuan , Zou Junliang , Liu Tianbo , Yin Huaqun
Published: May 2023

Virus-borne auxiliary genes have been demonstrated to influence their hosts’ characteristics and flexibility, resulting in an elevated ability to withstand diverse conditions and competition. However, the research on the soil viral community and its influencing factors is not extensive. The effects of bacterium-phage co-evolution in the metal contaminated environment have yet to be fully understood. In our research, numerous viral genes with conserved functional residues/motifs working on detoxifying heavy metals were pinpointed in the virome recovered from 58 contaminated soil metagenomic samples of mineral area, including tellurite resistance genes (e.g., terC, terD), copper resistance genes (e.g., copC, copA), and arsenate resistance genes (e.g., arsC). Moreover, viral proteins involved in key processes of nutrient uptake and metabolism, cellular function, polysaccharides production and biomineralization were also detected, which may also contribute to the response of microbial community to heavy metal stress. Our research showed that viruses have helped their hosts to acquire novel metal-resistance abilities through horizontal gene transfer (HGT) during the adaptation to challenging metal-rich environments. This was also evidenced by the high Codon adaption index (CAI) values across metal-resistance-related genes. This study has advanced our understanding on virus-host interplay within heavy metal contaminated soils.
Therapeutic approach of natural products that treat osteoporosis by targeting epigenetic modulation
Authors: Zhang Guokai , Liu Zhenying , Li Zihan , Zhang Bing , Yao Pengyu , Qiao Yun
Published: May 2023

Osteoporosis (OP) is a metabolic disease that affects bone, resulting in a progressive decrease in bone mass, quality, and micro-architectural degeneration. Natural products have become popular for managing OP in recent years due to their minimal adverse side effects and suitability for prolonged use compared to chemically synthesized products. These natural products are known to modulate multiple OP-related gene expressions, making epigenetics an important tool for optimal therapeutic development. In this study, we investigated the role of epigenetics in OP and reviewed existing research on using natural products for OP management. Our analysis identified around twenty natural products involved in epigenetics-based OP modulation, and we discussed potential mechanisms. These findings highlight the clinical significance of natural products and their potential as novel anti-OP therapeutics.
Flash heating boosts the potential for mechanochemical energy sources for subglacial ecosystems
Authors: Stone Jordan , Edgar John O. , Rutherford Johnny , Gill-Olivas Beatriz , Tranter Martyn , Gould Jamie A. , Xavier Cijo M. , Telling Jon
Published: May 2023

Subglacial environments harbour a diversity of microbial ecosystems capable of influencing biogeochemical cycles. However, the darkness and isolation of subglacial environments limit the energy sources available for microbial metabolism. A recently recognised energy source for these microbes in wet-based regions is the rock-water reactions that occur after the mechanical fracturing of glacial bedrock. These mechanochemical reactions produce H2 and H2O2 at 0°C from reactions with mineral surface defects (Si• and SiO•) and release Fe from within the mineral structures, providing electron donors and acceptors for microbial metabolism. However, the production of H2O2 and H2 may be underestimated as temperatures at rock abrasion sites can increase substantially above 0°C as glaciers “slip and grind” rocks, potentially accelerating the rates of mechanochemical reactions. Despite this, the effect of rapid heating on subsequent low-temperature mechanochemical reactions has yet to be examined. Here, we investigate H2, H2O2, and Fe production during low-temperature (0 °C) incubations of water with a range of ground rocks and minerals following “flash heating” to 30, 60, or 121 °C. We show that transient increases (as little as 5–10 min of heating) to moderate temperatures (30 or 60 °C) can significantly increase the rate of H2 production, while short-term heating to 121 °C generates larger bursts of H2. In addition, pyrite is easily crushed, potentially releasing large quantities of Fe2+ into subglacial systems and promoting mechanochemical reactions due to the resulting large surface area (10× larger than other materials). We provide the first evidence for H2 production from water reactions with crushed pyrite and suggest that crushed pyrite has a greater influence on subglacial H2O2 production than silicates. We conclude that electron donors in the form of Fe2+ and H2 bursts can be produced in subglacial ecosystems, which may be coupled to substantial concentrations of H2O2 produced from crushed pyrite. This suggests that rock–water mechanochemical reactions may be a greater source of energy for subglacial environments than previously recognised.
Risk prediction model construction for post myocardial infarction heart failure by blood immune B cells
Authors: Sun HouRong , Kong XiangJin , Wei KaiMing , Hao Jie , Xi Yue , Meng LingWei , Li GuanNan , Lv Xin , Zou Xin , Gu XingHua
Published: May 2023

Background

Myocardial infarction (MI) is a common cardiac condition with a high incidence of morbidity and mortality. Despite extensive medical treatment for MI, the development and outcomes of post-MI heart failure (HF) continue to be major factors contributing to poor post-MI prognosis. Currently, there are few predictors of post-MI heart failure.

Methods

In this study, we re-examined single-cell RNA sequencing and bulk RNA sequencing datasets derived from the peripheral blood samples of patients with myocardial infarction, including patients who developed heart failure and those who did not develop heart failure after myocardial infarction. Using marker genes of the relevant cell subtypes, a signature was generated and validated using relevant bulk datasets and human blood samples.

Results

We identified a subtype of immune-activated B cells that distinguished post-MI HF patients from non-HF patients. Polymerase chain reaction was used to confirm these findings in independent cohorts. By combining the specific marker genes of B cell subtypes, we developed a prediction model of 13 markers that can predict the risk of HF in patients after myocardial infarction, providing new ideas and tools for clinical diagnosis and treatment.

Conclusion

Sub-cluster B cells may play a significant role in post-MI HF. We found that the STING1, HSPB1, CCL5, ACTN1, and ITGB2 genes in patients with post-MI HF showed the same trend of increase as those without post-MI HF.
Exhaustion of CD8+ central memory responder T cell differentiation provokes non-melanoma skin cancer in elderly kidney transplant recipients
Authors: Leonhard Jonas , Schaier Matthias , Kälble Florian , Zeier Martin , Steinborn Andrea
Published: May 2023

Introduction

Immunosuppressive therapy prevents graft rejection but increases the risk of non-melanoma skin cancer (NMSC), especially in elderly kidney transplant recipients (KTR).

Methods

In this study, we separately investigated the differentiation of CD8+ regulatory T cells (Tregs) and responder T cells (Tresps) between healthy KTR without NMSC, KTR developing de-novo NMSC within two years after the enrolment, and KTR with NMSC at the time of enrolment. Antigen-unexperienced CCR7+CD45RA+CD31+ recent thymic emigrant (RTE) cells differentiate via CD45RA-CD31+ memory (CD31+ memory) cells, via resting mature naïve (MN) cells or via direct proliferation into CD45RA-CD31- memory (CD31- memory) cells, consisting of both CCR7+CD45RA- central memory (CM) and CCR7-CD45RA- effector memory (EM) cells.

Results

We found that both RTE Treg and Tresp differentiation via CD31+ memory Tregs/Tresps was age-independently increased in KTR, who developed de novo NMSC during the follow-up period, causing abundant CM Treg/Tresp production, which may be crucial for cancer immunity. These changes favored a strongly increased CD8+ Treg/Tresp ratio, suggesting this ratio as a reliable marker for de-novo NMSC development in KTR. However, with age, this differentiation was replaced by increased conversion of resting MN Tregs/Tresps into CM Tregs/Tresps, which exhausted for Tresps but not for Tregs. In KTR with already existing NMSC at enrolment, differentiation was maintained via conversion and proliferation of resting MN Tregs/Tresps, which however increasingly exhausted with age, especially for Tresps. This resulted in a strong accumulation of terminally differentiated effector memory (TEMRA) Tresps in elderly individuals. Patients with NMSC recurrence showed increased proliferation of resting MN Tregs/Tresps into EM Tregs/Tresps, which tended to exhaust more rapidly, particularly for Tresps, than in patients without NMSC recurrence.

Discussion

In conclusion, we provide evidence that immunosuppressive therapy inhibits differentiation of CD8+ Tregs more than that of CD8+ Tresps, resulting in an exhausted Tresp profile, thus providing a possible therapeutic approach to improve poor cancer immunity in elderly KTR.
Pneumatosis cystoides intestinalis in dermatomyositis: a case series report and literature review
Authors: Liu Jianwen , Zhang Ling , Chen Shuo , Lu Xin , Li Shanshan
Published: May 2023

Pneumatosis cystoides intestinalis (PCI) in adult dermatomyositis (DM) is rarely described. This report aimed to describe the clinical features and prognosis of PCI in six adult patients with DM (four with anti-MDA5 antibodies, one with anti-SAE antibodies, and one with anti-TIF-1γ antibodies). Except for one patient with transient abdominal pain, the remaining five patients were asymptomatic. PCI occurred in the ascending colon in all patients, of whom five had free gas in the abdominal cavity. No patients received excessive treatment, and PCI disappeared in four patients during the follow-up. Additionally, we reviewed previous studies on this complication.
Successful treatment of a refractory intestinal Behcet’s disease with an oncology history by Vedolizumab: a case report and literature review
Authors: Li Ruixia , Li Xiaofei , Zhou He , Shi Yanting , Wang Fang , Wu Tong , Liang Jie
Published: May 2023

Objective

Behçet’s Disease (BD) is an intractable systemic vasculitis. When accompanied by intestinal symptoms, the prognosis is usually poor. 5-Aminosalicylic acid (5-ASA), corticosteroids, immunosuppressive drugs, and anti-tumor necrosis factor-α (anti-TNF-α) biologics are standard therapies to induce or maintain remission for intestinal BD. However, they might not be effective in refractory cases. Safety should also be considered when patients have an oncology history. Regarding the pathogenesis of intestinal BD and the specific targeting effect of vedolizumab (VDZ) on the inflammation of the ileum tract, previous case reports suggested that VDZ might be a potential treatment for refractory intestinal BD.

Methods

We report a 50-year-old woman patient with intestinal BD who had oral and genital ulcers, joint pain, and intestinal involvement for about 20 years. The patient responds well to anti-TNF-α biologics but not to conventional drugs. However, biologics treatment was discontinued due to the occurrence of colon cancer.

Results

VDZ was intravenously administered at a dose of 300 mg at 0, 2, and 6 weeks and then every eight weeks. At the 6-month follow-up, the patient reported significant improvement in abdominal pain and arthralgia. We observed complete healing of intestinal mucosal ulcers under endoscopy. However, her oral and vulvar ulcers remained unresolved, which disappeared after adding thalidomide.

Conclusion

VDZ may be a safe and effective option for refractory intestinal BD patients who do not respond well to conventional treatments, especially those with an oncology history.
All roads lead to Rome: inter-origin variation in metabolomics reprogramming of the northern shrimp exposed to global changes leads to a comparable physiological status
Authors: Guscelli Ella , Chabot Denis , Vermandele Fanny , Madeira Diana , Calosi Piero
Published: May 2023

Impacts of global ocean changes on species have historically been investigated at the whole-organism level. However, acquiring an in-depth understanding of the organisms’ cellular metabolic responses is paramount to better define their sensitivity to environmental challenges. This is particularly relevant for species that experience highly different environmental conditions across their distribution range as local acclimatization or adaptation can influence their responses to rapid global ocean changes. We aimed at shedding light on the cellular mechanisms underpinning the sensitivity to combined ocean warming (OW) and acidification (OA) in the northern shrimp Pandalus borealis, from four different geographic origins defined by distinctive environmental regimes in the northwest Atlantic: i.e. St. Lawrence Estuary (SLE), Eastern Scotian Shelf (ESS), Esquiman Channel (EC) and Northeast Newfoundland Coast (NNC). We characterized targeted metabolomics profiles of the muscle of shrimp exposed to three temperatures (2, 6 or 10°C) and two pH levels (7.75 or 7.40). Overall, shrimp metabolomics profiles were modulated by a significant interaction between temperature, pH and origin. Temperature drove most of the metabolomics reprogramming, confirming that P. borealis is more sensitive to OW than OA. Inter-origin differences in metabolomics profiles were also observed, with temperature*pH interactions impacting only shrimp from SLE and ESS, pH affecting only shrimp from SLE and temperature impacting shrimp from all origins. Temperature influenced metabolomics pathways related to the tricarboxylic acid cycle (TCA) and amino acid metabolism, resulting mainly in an accumulation of TCA intermediates and tyrosine. Temperature*pH and pH in isolation only affected amino acid metabolism, leading to amino acids accumulation under low pH. However, the ratio of ATP : ADP remained constant across conditions in shrimp from all origins suggesting that their energetic status is not affected by OW and OA. Still, the accumulation of TCA intermediates and tyrosine suggests the possible enhancement of immune responses under future OW and OA conditions. Our findings suggest that shrimp from SLE are more sensitive at the molecular level, compared to others, to future complex environmental conditions. This underlines the importance of investigating intraspecific variation in mechanisms of responses to combined drivers when trying to define species’ sensitivity to global ocean changes.
Pancreatic cancer and exosomes: role in progression, diagnosis, monitoring, and treatment
Authors: Fang Xingliang , Lan Huanrong , Jin Ketao , Qian Jun
Published: May 2023

Pancreatic cancer (PC) is one of the most dangerous diseases that threaten human life, and investigating the details affecting its progression or regression is particularly important. Exosomes are one of the derivatives produced from different cells, including tumor cells and other cells such as Tregs, M2 macrophages, and MDSCs, and can help tumor growth. These exosomes perform their actions by affecting the cells in the tumor microenvironment, such as pancreatic stellate cells (PSCs) that produce extracellular matrix (ECM) components and immune cells that are responsible for killing tumor cells. It has also been shown that pancreatic cancer cell (PCC)-derived exosomes at different stages carry molecules. Checking the presence of these molecules in the blood and other body fluids can help us in the early stage diagnosis and monitoring of PC. However, immune system cell-derived exosomes (IEXs) and mesenchymal stem cell (MSC)-derived exosomes can contribute to PC treatment. Immune cells produce exosomes as part of the mechanisms involved in the immune surveillance and tumor cell-killing phenomenon. Exosomes can be modified in such a way that their antitumor properties are enhanced. One of these methods is drug loading in exosomes, which can significantly increase the effectiveness of chemotherapy drugs. In general, exosomes form a complex intercellular communication network that plays a role in developing, progressing, diagnosing, monitoring, and treating pancreatic cancer.
Mediating immunosuppressive functions: a new perspective on the complex immunological properties of SEMA4D in the tumor microenvironment
Authors: Zeng Shujie , Zhang Zihao , Ye Chunshui , Wang Jinshen , Jing Changqing , Li Leping
Published: May 2023

Semaphorin 4D (SEMA4D) is considered a new antitumor target closely related to immune cells. However, understanding the role of SEMA4D in the tumor microenvironment (TME) is limited. In this study, we explored the expression and immune cell infiltration patterns of SEMA4D using multiple bioinformatics datasets and analyzed the relationship between SEMA4D expression with immune checkpoints, tumor mutational load (TMB), microsatellite instability (MSI) and immune function. We detected that SEMA4D is overexpressed in many tumors types, widely enriched in immune cells, and closely associated with TILs, MSI, TMB, as well as T-cell exhaustion-associated immune checkpoints, and thus can broadly affect the immune microenvironment. We further verified the overexpression of SEMA4D in tumor and its distribution in TME by immunohistochemistry, RT-qPCR and flow cytometry, and confirmed that decreased expression of SEMA4D can lead to recovery of T cell exhaustion. In conlusion, this study provides a more comprehensive perspective of SEMA4D regulation of tumor immunity, which provide a new option for cancer immunotherapy.
Overall survival comparison between pediatric and adult Ewing sarcoma of bone and adult nomogram construction: a large population-based analysis
Authors: Hsu Chi-Jen , Ma Yongguang , Xiao Peilun , Hsu Chia-Chien , Wang Dawei , Fok Mei Na , Peng Rong , Xu Xianghe , Lu Huading
Published: May 2023

Background

Ewing sarcoma (ES) is a common primary bone tumor in children. Our study aimed to compare overall survival (OS) between pediatric and adult bone ES patients, identify independent prognostic factors and develop a nomogram for predicting OS in adult patients with ES of bone.

Methods

We retrospectively analyzed data for the 2004–2015 period from the Surveillance, Epidemiology, and End Results (SEER) database. To guarantee well-balanced characteristics between the comparison groups, propensity score matching (PSM) was used. Kaplan–Meier (KM) curves were used to compare OS between pediatric and adult patients with ES of bone. Univariate and multivariate Cox regression analyses were used to screen independent prognostic factors for ES of bone, and a prognostic nomogram was constructed by using the factors identified. The prediction accuracy and clinical benefit were evaluated using receiver operating characteristic (ROC) curves, areas under the curves (AUCs), calibration curves, and decision curve analysis (DCA).

Results

Our results showed that adult ES patients had lower OS than younger ES patients. Age, surgery, chemotherapy, and TNM stage were independent risk factors for bone ES in adults and were used to develop a nomogram. AUCs for 3-, 5-, and 10-year OS were 76.4 (67.5, 85.3), 77.3 (68.6, 85.9) and 76.6 (68.6, 84.5), respectively. Calibration curves and DCA results indicated excellent performance for our nomogram.

Conclusion

We found that ES pediatric patients have better OS than adult ES patients, and we constructed a practical nomogram to predict the 3-, 5- and 10-year OS of adult patients with ES of bone based on independent prognostic factors (age, surgery, chemotherapy, T stage, N stage and M stage).
Morphologic classification of tracheobronchial arborization in children with congenital tracheobronchial stenosis and the associated cardiovascular defects
Authors: Hu Jie , Wang Hao , Du Xinwei , Zhu Limin , Wang Shunmin , Zhang Haibo , Xu Zhiwei , Chen Hao
Published: May 2023

Background

We sought to classify patients with congenital tracheal stenosis (CTS) according to tracheobronchial morphology and determine anatomic features associated with tracheobronchial anomalies (TBAs) and concurrent cardiovascular defects (CVDs).

Methods

We enrolled 254 patients who underwent tracheoplasty between November 1, 2009 and December 30, 2018. The anatomic features of the tracheobronchial tree and cardiovascular system were abstracted from bronchoscopy, echocardiography, computerized tomography, and operative reports.

Results

Four types of tracheobronchial morphology were identified: Type-1, which included normal tracheobronchial arborization (Type-1A, n = 29) and tracheal bronchus (Type-1B, n = 22); Type-2 (tracheal trifurcation; n = 49), and Type-3 (typical bridging bronchus; n = 47). Type-4 (bronchus with an untypical bridging pattern) was divided into Type-4A (involving bronchial diverticulum; n = 52) and Type-4B (absent bronchus; n = 55). Carinal compression and tracheomalacia were significantly more frequent in Type-4 patients than in the other patients (P < 0.01). CVDs were common in patients with CTS, especially in patients with Type-3 and Type-4 (P < 0.01). Persistent left superior vena cava was most common among patients with Type-3 (P < 0.01), and pulmonary artery sling was most frequent among those with Type-4 (P < 0.01). Outflow tract defects were most likely to occur in Type-1B. Early mortality was detected in 12.2% of all patients, and young age (P = 0.02), operation in the early era (P < 0.01), and bronchial stenosis (P = 0.03) were proven to be risk factors.

Conclusions

We demonstrated a useful morphological classification for CTS. Bridging bronchus was most closely linked with vascular anomalies, while tracheal bronchus was frequently associated with outflow tract defects. These results may provide a clue to CTS pathogenesis.
The perception of pediatric sickle cell anemia patient's caregivers toward hematopoietic stem cell transplantation (single-center experience, Saudi Arabia)
Authors: Monagel Dania A. , Alemam Naglla , Betar Manar , Alnafisi Fay N. , Faidah Dania E. , Aloteibi Reema E. , Khan Muhammad , Malli Israa A.
Published: May 2023

Background

Sickle cell disease (SCD) is a relatively common genetic disorder in Saudi Arabia characterized by the predominance of sickle hemoglobin (HbS). Although multiple supportive care options exist for patients with SCD, hematopoietic stem cell transplantation (HSCT) is the only cure available and has become highly successful, with an almost 91% overall survival rate. However, pursuing this procedure is still restrained as a curative treatment option. Therefore, this study aimed to evaluate the perception of parents' caregivers at the National Guard Hospital pediatric hematology clinic regarding using HSCT as a curative approach for their children with SCD.

Methods

This is a cross-sectional study of the interviewer-administered survey distributed utilizing electronic devices to caregivers of pediatric patients with SCD. Subjects were recruited from Pediatric Hematology & Oncology clinics at National Guard Hospital Affairs in King Abdulaziz Medical City, Jeddah, Saudi Arabia. An estimated sample size of 100 was initially calculated out of 140 pediatric SCD patients; 72 responses were collected from participants. All study participants gave informed consent. All results were analyzed using SPSS; moreover, statistics were set at a CI of 95% and a p < 0.05. In addition, inferential and descriptive statistics were done.

Results

Of all respondents, 42 (67.8%) would accept HSCT if their hematologist recommended it. However, approximately 7 (11.3%) were not interested in the procedure, and the rest, 13 (21%), were uncertain. The most reported reasons for HSCT rejection among all respondents were attributed to side effects 31 (50.8%), lack of knowledge 8 (13.1%), and misconception toward the procedure 22 (36.1%).

Conclusion

The results of this study were consistent with the fact that most caregivers would follow along with HSCT if it seemed to be fit and was recommended by their hematologists. However, to the best of our knowledge, our study being the first of its kind in the region, further research in the kingdom on the perception of HSCT is needed. Nonetheless, further patient education, an increase in caregivers' knowledge, and enlightenment of the medical team on HSCT as a curative option for sickle cell disease are vital.
Schizandrin C regulates lipid metabolism and inflammation in liver fibrosis by NF-κB and p38/ERK MAPK signaling pathways
Authors: Chen Panpan , Wang Rong , Liu Fangbin , Li Shengnan , Gu Yanqiu , Wang Lei , Yuan Yongfang
Published: May 2023

Liver fibrosis is considered a sustained wound healing response and metabolic syndrome, and its therapy is of great significance for chronic liver disease. Schizandrin C, as one lignan from hepatic protectant Schisandra chinensis, can depress the oxidative effect and lipid peroxidation, and protect against liver injury. In this study, C57BL/6J mice were used to estimate a liver fibrosis model by CCl4, and Schizandrin C exerted an anti-hepatic fibrosis effect, as evidenced by decreased alanine aminotransferase, aspartate aminotransferase and total bilirubin activities in serum, lower hydroxyproline content, recuperative structure and less collagen accumulation in the liver. In addition, Schizandrin C reduced the expressions of alpha-smooth muscle actin and type Ι collagen in the liver. In vitro experiments also revealed that Schizandrin C attenuated hepatic stellate cell activation in both LX-2 and HSC-T6 cells. Furthermore, lipidomics and quantitative real-time PCR analysis revealed that Schizandrin C regulated the lipid profile and related metabolic enzymes in the liver. In addition, the mRNA levels of inflammation factors were downregulated by Schizandrin C treatment, accompanied by lower protein levels of IκB-Kinase-β, nuclear factor kappa-B p65, and phospho-nuclear factor kappa-B p65. Finally, Schizandrin C inhibited the phosphorylation of p38 MAP kinase and extracellular signal-regulated protein kinase, which were activated in the CCl4 fibrotic liver. Taken together, Schizandrin C can regulate lipid metabolism and inflammation to ameliorate liver fibrosis by nuclear factor kappa-B and p38/ERK MAPK signaling pathways. These findings supported Schizandrin C as a potential drug for liver fibrosis.
Ripretinib in combination with tyrosine kinase inhibitor as a late-line treatment option for refractory gastrointestinal stromal tumors: two case reports and literature review
Authors: Zhang Yefan , Huang Zhen
Published: May 2023

Background: This case report presents two clinical cases of metastatic refractory gastrointestinal stromal tumor (GIST) with treatment history of 6–14 years. The follow-up treatment of both cases comprised ripretinib dose escalation and its combination with other tyrosine kinase inhibitors (TKIs). To the best of our knowledge, this is the first report that explored ripretinib combination therapy in the late-line treatment of GISTs.

Case description: Case-1 represents a 57-year-old female patient who underwent surgical resection for retroperitoneal GIST in 2008. After tumor recurrence in 2009, imatinib was started with complete response for 8 years. Imatinib was followed by sunitinib and regorafenib treatment. In March 2021, due to progressive disease (PD), the patient started ripretinib (150 mg QD) and achieved partial response (PR). Six months later, the patient showed PD. Subsequently, ripretinib dose was increased (150 mg BID) followed by ripretinib (100 mg QD) and imatinib (200 mg QD) combination. CT performed in February 2022 revealed stable lesions with internal visible necrosis. Combination therapy achieved stable disease (SD) for 7 months. On further follow-up in July 2022, the patient showed PD and died in September 2022.

Case-2: represents a 73-year-old female patient diagnosed with unresectable duodenal GIST with liver, lung, and lymph node metastases in 2016. After treatment with imatinib, followed by sunitinib, regorafenib, and imatinib rechallenge, ripretinib (150 mg QD) was administered in May 2021, and SD was achieved. Ripretinib dose was increased (200 mg QD) due to PD in December 2021. The tumor showed heterogeneous manifestations, with overall size increase and regression in right posterior lobe. In February 2022, ripretinib (150 mg) plus sunitinib (25 mg) QD was commenced. On follow-up in April 2022, the patient showed slightly improved symptoms with stable hematologic parameters. Combination therapy achieved SD for 5 months and the patient showed PD in July 2022 and discontinued the treatment later. The patient was in poor general condition and was receiving nutritional therapy until last follow-up in October 2022.

Conclusion: This case report provides evidence that combination therapy of ripretinib with other TKIs could be an effective late-line treatment option for refractory GIST patients.
Morphologic and genic effects of waste pollution on the reproductive physiology of Paracentrotus lividus lmk: a mesocosm experiment
Authors: Glaviano Francesca , Federico Serena , Pinto Bruno , Gharbi Maissa , Russo Tania , Cosmo Anna Di , Polese Gianluca , Costantini Maria , Zupo Valerio
Published: May 2023

A considerable amount of coastal contamination is caused by wastes deriving from household and the degradation and the metabolism of plants and animals, even if our attention is commonly focused on industrial pollutants and contaminants. Waste pollutants are mainly represented by highly diluted soluble compounds and particles deriving from dead organisms. This complex combination, consisting of suspended particles and dissolved nutrients, has a significant impact on coastal planktonic and benthic organisms, also playing an active role in the global cycles of carbon. In addition, production practices are nowadays shifting towards recirculated aquaculture systems (RAS) and the genic responses of target organisms to the pollution deriving from animal metabolism are still scarcely addressed by scientific investigations. The reservoir of organic matter dissolved in the seawater is by far the least understood if compared to that on land, cause only a few compounds have been identified and their impacts on animals and plants are poorly understood. The tendency of these compounds to concentrate at interfaces facilitates the absorption of dissolved organic compound (DOC) onto suspended particles. Some DOC components are chemically combined with dissolved metals and form complexes, affecting the chemical properties of the seawater and the life of the coastal biota. In this research, we compared the reproductive performances of the common sea urchin Paracentrotus lividus cultured in open-cycle tanks to those cultured in a recirculating aquaculture system (RAS), where pollution progressively increased during the experiment due to animal escretions. Sea urchins were cultured for 7 months under these two conditions and their gametes were collected. Embryos resulting by in vitro fertilization were analyzed by Real Time qPCR to identify possible effects of pollution-induced stress. The fertility of sea urchins was evaluated, as well as the gonadosomatic indices and the histological features of gonads. Our results indicate that pollution due to excess of nutrients, event at sub-lethal concentrations, may hardly impact the reproductive potential of this key species and that chronic effects of stress are revealed by the analyses of survival rates and gene expression.
Exploring the neurobiology of Merge at a basic level: insights from a novel artificial grammar paradigm
Authors: Liu Yang , Gao Chenyang , Wang Peng , Friederici Angela D. , Zaccarella Emiliano , Chen Luyao
Published: May 2023

Introduction

Human language allows us to generate an infinite number of linguistic expressions. It’s proposed that this competence is based on a binary syntactic operation, Merge, combining two elements to form a new constituent. An increasing number of recent studies have shifted from complex syntactic structures to two-word constructions to investigate the neural representation of this operation at the most basic level.

Methods

This fMRI study aimed to develop a highly flexible artificial grammar paradigm for testing the neurobiology of human syntax at a basic level. During scanning, participants had to apply abstract syntactic rules to assess whether a given two-word artificial phrase could be further merged with a third word. To control for lower-level template-matching and working memory strategies, an additional non-mergeable word-list task was set up.

Results

Behavioral data indicated that participants complied with the experiment. Whole brain and region of interest (ROI) analyses were performed under the contrast of “structure > word-list.” Whole brain analysis confirmed significant involvement of the posterior inferior frontal gyrus [pIFG, corresponding to Brodmann area (BA) 44]. Furthermore, both the signal intensity in Broca’s area and the behavioral performance showed significant correlations with natural language performance in the same participants. ROI analysis within the language atlas and anatomically defined Broca’s area revealed that only the pIFG was reliably activated.

Discussion

Taken together, these results support the notion that Broca’s area, particularly BA 44, works as a combinatorial engine where words are merged together according to syntactic information. Furthermore, this study suggests that the present artificial grammar may serve as promising material for investigating the neurobiological basis of syntax, fostering future cross-species studies.
Research on the construction of the evaluation index system of game-based teaching
Authors: Wang Yunhong , Zhang Sujing , Hong Hetiao
Published: May 2023

This research aims to combine the pursuit of literacy cultivation with a focused investigation into the evidence of game-based teaching (GBT). To achieve this, the study employs a mixed-methods approach including the interview method, Delphi method, and network hierarchical analysis (ANP) to analysis Expert opinions and construct a comprehensive GBT evaluation index system. The results indicated that a comprehensive GBT evaluation index system is comprised of five primary indicators: teaching objectives, game-based teaching methods, teaching content, game-based teaching processes, and game-based teaching characteristics. Additionally, there are 19 secondary indicators, such as objective content, game presentation, context construction, and flow experience. This study expects to effectively capture the unique attributes of game-based classes and to assist teachers in improving the design of GBT activities in practical applications.
Deep space debris—Detection of potentially hazardous asteroids and objects from the southern hemisphere
Authors: Kruzins Ed , Benner Lance , Boyce Russell , Brown Melrose , Coward David , Darwell Sam , Edwards Phil , Elizabeth-Glina Lauren , Giorgini Jon , Horiuchi Shinji , Lambert Andrew , Lazio Joseph , Calves Guifre Molera , Moore John , Peters Edwin , Phillips Chris , Stevens Jamie , Verveer Arie
Published: May 2023

Space debris are composed of both natural and human made objects, some in near Earth orbits while others are passing through deep space. Asteroids may represent one form of near Earth and deep space debris. In this article we report on a set of asteroid observations from the southern hemisphere. We indicate that Apollo and Aten class asteroids represent another form of deep space debris of a potentially hazardous nature to orbiting spacecraft and/or Earth based locations. We also show some of the operational challenges, types of facilities and the importance of geographic diversity, that is, necessary for detecting, observing and characterising asteroids, especially PHA’s. For many years, space agencies and institutions have observed and monitored near Earth asteroids and objects (NEO’s) using high gain radio frequency antennas and optical telescopes in the northern hemisphere (GSSR, Arecibo, Catalina, Pan-STARRS, Atlas and Linear) 1) However a regular operational system to monitor the southern skies does not have the same level of maturity and is where a percentage of asteroids and various human made objects are not detected until they pass into northern skies. To fill that gap the Southern Hemisphere Asteroid Radar Program (SHARP) 2) located in Australia uses available antenna time on either a 70 or 34 m beam waveguide antenna located at the Canberra Deep Space Communication Complex (CDSCC) to transmit a Doppler compensated continuous radio wave at 2.114 GHz (14.2 cm) and 7.15945 GHz (4.2 cm) toward the NEO and receive its echoes at the 64 m Parkes or 6 m × 22 m Australia Telescope Compact Array (ATCA) antennas at Narrabri in Australia. This mode of NEO observation is termed a deep space bistatic radar. The southern hemisphere program has also recently been joined by the 12 m University of Tasmania antennas at Hobart (Tasmania) and Katherine (Northern Territory). Combining SHARPS bistatic radar with small optical apertures located at the University of New South Wales (UNSW) and University of Western Australia (UWA) allows combined optical/RF NEO detections. Whilst sub-metre class optical instruments have contributed independently to asteroid detection over decades, the use of coordinated small 0.3–0.5 m instruments synchronized to large asteroid radars offers an observational flexibility and adaptability when larger optical systems 3) are dedicated to other forms of professional optical astronomy. Since 2015, SHARP has illuminated and tracked over 30 NEO’s ranging in diameter from 7 to 5000 m at ranges of 0.1–18 lunar distances (LD) from Australia.
Re-expansion of vertebral compression fractures in patients with multiple myeloma with percutaneous vertebroplasty using spinejack implants: a preliminary and retrospective study
Authors: Pusceddu Claudio , Faiella Eliodoro , Derudas Daniele , Ballicu Nicola , Melis Luca , Zedda Stefano , Marsico Salvatore
Published: May 2023

Objective

To retrospectively evaluate the feasibility and effectiveness of vertebroplasty using Spinejack implantation for the treatment and stabilization of painful vertebral compression fractures, in patients diagnosed with Multiple Myeloma (MM), to allow both an effective pain reduction and a global structural spine stabilization.

Materials and Methods

From July 2017 and May 2022 thirty-nine patients diagnosed MM, with forty-nine vertebral compression fractures underwent percutaneous Vertebroplasty using Spinejack Implants. We analyzed the feasibility and complications of the procedure, the decrease in pain using visual analogue scale (VAS) and Functional Mobility Scale (FMS).

Results

The technical success rate was 100%. No procedure-related major complications or death occurred. In the 6-month follow-up, the mean VAS score decreased from 5.4 ± 1.0 to 0.2 ± 0.5 with a mean reduction of 96.3%. FMS decreased from 2.3 ± 0.5 vs. 1.2 ± 0.4 with a mean reduction of −47.8%. There were no major complications related to incorrect positioning of the Expandable Titanium SpineJack Implants. In five patients, a cement leak was observed with no associated clinical manifestations. The average length of hospital stay was 6–8 Hours6.6 ± 1.2 h. No new bone fractures or local disease recurrence occurred during a median contrast-enhanced CT follow-up of 6 months.

Conclusions

Our results suggest that vertebroplasty, using Spinejack implantation for the treatment and stabilization of painful vertebral compression fractures, secondary to Multiple Myeloma is a safe and effective procedure with long - term pain relief and restoration of vertebral height.
Innovation in gastrointestinal surgery: the evolution of minimally invasive surgery—a narrative review
Authors: Walshaw Josephine , Huo Bright , McClean Adam , Gajos Samantha , Kwan Jing Yi , Tomlinson James , Biyani Chandra Shekhar , Dimashki Safaa , Chetter Ian , Yiasemidou Marina
Published: May 2023

Background

Minimally invasive (MI) surgery has revolutionised surgery, becoming the standard of care in many countries around the globe. Observed benefits over traditional open surgery include reduced pain, shorter hospital stay, and decreased recovery time. Gastrointestinal surgery in particular was an early adaptor to both laparoscopic and robotic surgery. Within this review, we provide a comprehensive overview of the evolution of minimally invasive gastrointestinal surgery and a critical outlook on the evidence surrounding its effectiveness and safety.

Methods

A literature review was conducted to identify relevant articles for the topic of this review. The literature search was performed using Medical Subject Heading terms on PubMed. The methodology for evidence synthesis was in line with the four steps for narrative reviews outlined in current literature. The key words used were minimally invasive, robotic, laparoscopic colorectal, colon, rectal surgery.

Conclusion

The introduction of minimally surgery has revolutionised patient care. Despite the evidence supporting this technique in gastrointestinal surgery, several controversies remain. Here we discuss some of them; the lack of high level evidence regarding the oncological outcomes of TaTME and lack of supporting evidence for robotic colorectalrectal surgery and upper GI surgery. These controversies open pathways for future research opportunities with RCTs focusing on comparing robotic to laparoscopic with different primary outcomes including ergonomics and surgeon comfort.
Toponimy koreańskie w polszczyźnie – źródła
transpozycji, systemy transkrypcyjne i strategie asymilacji fonicznej
Authors: Gwangseok Kim , Pietrow Jarosław A.
Published: May 2023

The intensively developing Polish-Korean contacts and direct access to the knowledge of South Korea as one of the major East Asian countries obtained from Internet and media coverage have resulted in Korean toponyms being assimilated in the Polish language to an increasing extent. However, they are adapted in a number of ways, being mediated through two main systems of Latinising the Korean language, i.e. the McCune-Reischauer transcription and the modified MOE transcription, which is officially propagated by Korean authorities. This leads to a certain competition between the two systems, which are, moreover, often used in the situation of not only lack of command of the source language itself, but also ignorance of the conventional rules of the Latin transcription of Korean geographical names, which are different in both transcriptions. Moreover, toponyms must be assimilated based on various compromises between the phonological system of the source language and the system of the receiving language. Such a helpful compromise might be an additionally used Polonised transcription in the resource of letter symbols, but this requires substantial adjustments and additions as well as unavoidable concessions to make it easier for Polish recipients to decipher texts about Korea. From the perspective of historical standardisation of some characteristic Korean toponyms in Polish publications, various transcription tools, methods of their assimilation and adaptations, as well as the obvious cognitive barriers might become definitely more pronounced and informative for both text authors and recipients
Komunikacja z czytelnikiem w sytuacji kryzysu czytelnictwa końca XVII
i początku XVIII wieku
Authors: Rodek Ewa
Published: May 2023

This article is devoted to issues in the literary culture of the Polish–Lithuanian Commonwealth of the end of the 17th and the beginning of the 18th centuries. The material of prefaces to an anonymous recipient attached to works of 1675–1739 served as the basis for analysing the statements concerning the process of reception and the act of reading. The results of the analysis indicate that the authors of the prefaces perceived a crisis of readership and interest in books. To this end, they activated the audience for attentive and engaged reading. Moreover, the authors declared that they adapted the form and content of the works to the needs and tastes of readers. Going beyond the traditionally established convention of using topical motifs, they otten extolled their off erings in direct, concise but succinct messages, which alludes to the characteristics of
advertising language.
Mentee, czyli mentorowany – o recepcji zapożyczenia
w języku polskim
Authors: Grażul-Luft Agnieszka
Published: May 2023

The borrowing mentee occurs in the Polish language mainly in the area concerned with
professional development. The noun is semantically close e.g. to the word podopieczny, yet its emotional load seems to be more positive than that of other semantically similar lexemes. The borrowing mentee has not be recorded in dictionaries of Polish yet; its meaning has been discussed based on contextual usages.
Ciemności egipskie, kimeryjskie, zewnętrzne. Znaczenia jednostek
frazeologicznych na tle porównawczym
Authors: Breyvo Natalia
Published: May 2023

This article discusses the origin of Polish phraseological units for complete darkness (ciemności egipskie, ciemności kimeryjskie, ciemności zewnętrzne) compared to their Russian and English equivalents and presents the peculiarities of their meanings perceived from the angle of their sources and a texts of many varieties
Survey and Cost–Benefit Analysis of Sorting Technology for the Sweetpotato Packing Lines
Authors: Lu Yuzhen , Harvey Lorin , Shankle Mark
Published: May 2023

Supplying high-quality fresh sweetpotato roots to the consumer requires sorting the roots by quality and removing culls deemed unsuitable for fresh markets at packing facilities. The sorting operation is traditionally performed by manual labor. This study surveyed the sorting lines of seven commercial sweetpotato packinghouses in Mississippi during the packing season of 2021. Sorting for defects entirely relied on labor, which accounted for up to 50% of the total labor in packinghouses. A cost–benefit analysis was conducted to determine the cost-effectiveness of implementing automated sorting technology as an alternative to manual sorting. The net benefits of automated sorting depended on labor savings and equipment costs. Machines at or less than USD 100,000 were economically beneficial with payback periods of less than three years when four or more workers could be replaced, while machines of USD 350,000 and higher would be not justifiable when quick economical returns were sought. Automated sorting promises to increase the profitability and competitiveness of fresh market sweetpotato packing industries.