bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementation of a Savvy Mobile ECG Sensor for Heart Rhythm Disorder Screening at the Primary Healthcare Level: An Observational Prospective Study
Authors: Vodička Staša , Susič Antonija Poplas , Zelko Erika
Published: January 2021

Introduction: The Jozef Stefan Institute developed a personal portable electrocardiogram (ECG) sensor Savvy that works with a smartphone, and this was used in our study. This study aimed to analyze the usefulness of telecardiology at the primary healthcare level using an ECG personal sensor. Methods: We included 400 patients with a history of suspected rhythm disturbance who visited their family physician at the Healthcare Center Ljubljana and Healthcare Center Murska Sobota from October 2016 to January 2018. Results: The study found that there was no statistically significant difference between the test and control groups in the number of present rhythm disorders and actions taken to treat patients with either observation or administration of a new drug. However, in the test group, there were significantly fewer patients being referred to a cardiologist than in the control group (p < 0.001). Discussion: The use of an ECG sensor helps family physicians to distinguish between patients who need to be referred to a cardiologist and those who can be treated by them. This method is useful for both physicians and patients because it shortens the time taken to start treatment, can be used during pandemics such as COVID-19, and reduces unnecessary cost.
Capacitive Field-Effect Biosensor Studying Adsorption of Tobacco Mosaic Virus Particles
Authors: Jablonski Melanie , Poghossian Arshak , Severins Robin , Keusgen Michael , Wege Christina , Schöning Michael
Published: January 2021

Plant virus-like particles, and in particular, tobacco mosaic virus (TMV) particles, are increasingly being used in nano- and biotechnology as well as for biochemical sensing purposes as nanoscaffolds for the high-density immobilization of receptor molecules. The sensitive parameters of TMV-assisted biosensors depend, among others, on the density of adsorbed TMV particles on the sensor surface, which is affected by both the adsorption conditions and surface properties of the sensor. In this work, Ta2O5-gate field-effect capacitive sensors have been applied for the label-free electrical detection of TMV adsorption. The impact of the TMV concentration on both the sensor signal and the density of TMV particles adsorbed onto the Ta2O5-gate surface has been studied systematically by means of field-effect and scanning electron microscopy methods. In addition, the surface density of TMV particles loaded under different incubation times has been investigated. Finally, the field-effect sensor also demonstrates the label-free detection of penicillinase immobilization as model bioreceptor on TMV particles.
Kinetic Modeling and Meta-Analysis of the Bacillus subtilis SigB Regulon during Spore Germination and Outgrowth
Authors: Vohradsky Jiri , Schwarz Marek , Ramaniuk Olga , Ruiz-Larrabeiti Olatz , Vaňková Hausnerová Viola , Šanderová Hana , Krásný Libor
Published: January 2021

The exponential increase in the number of conducted studies combined with the development of sequencing methods have led to an enormous accumulation of partially processed experimental data in the past two decades. Here, we present an approach using literature-mined data complemented with gene expression kinetic modeling and promoter sequence analysis. This approach allowed us to identify the regulon of Bacillus subtilis sigma factor SigB of RNA polymerase (RNAP) specifically expressed during germination and outgrowth. SigB is critical for the cell’s response to general stress but is also expressed during spore germination and outgrowth, and this specific regulon is not known. This approach allowed us to (i) define a subset of the known SigB regulon controlled by SigB specifically during spore germination and outgrowth, (ii) identify the influence of the promoter sequence binding motif organization on the expression of the SigB-regulated genes, and (iii) suggest additional sigma factors co-controlling other SigB-dependent genes. Experiments then validated promoter sequence characteristics necessary for direct RNAP–SigB binding. In summary, this work documents the potential of computational approaches to unravel new information even for a well-studied system; moreover, the study specifically identifies the subset of the SigB regulon, which is activated during germination and outgrowth.
Metagenomics and Culture Dependent Insights into the Distribution of Firmicutes across Two Different Sample Types Located in the Black Hills Region of South Dakota, USA
Authors: Govil Tanvi , Paste Manasi , Samanta Dipayan , David Aditi , Goh Kian Mau , Li Xiangkai , Salem David R. , Sani Rajesh K.
Published: January 2021

Firmicutes is almost a ubiquitous phylum. Several genera of this group, for instance, Geobacillus, are recognized for decomposing plant organic matter and for producing thermostable ligninolytic enzymes. Amplicon sequencing was used in this study to determine the prevalence and genetic diversity of the Firmicutes in two distinctly related environmental samples—South Dakota Landfill Compost (SDLC, 60 °C), and Sanford Underground Research Facility sediments (SURF, 45 °C). Although distinct microbial community compositions were observed, there was a dominance of Firmicutes in both the SDLC and SURF samples, followed by Proteobacteria. The abundant classes of bacteria in the SDLC site, within the phylum Firmicutes, were Bacilli (83.2%), and Clostridia (2.9%). In comparison, the sample from the SURF mine was dominated by the Clostridia (45.8%) and then Bacilli (20.1%). Within the class Bacilli, the SDLC sample had more diversity (a total of 11 genera with more than 1% operational taxonomic unit, OTU). On the other hand, SURF samples had just three genera, about 1% of the total population: Bacilli, Paenibacillus, and Solibacillus. With specific regard to Geobacillus, it was found to be present at a level of 0.07% and 2.5% in SURF and SDLC, respectively. Subsequently, culture isolations of endospore-forming Firmicutes members from these samples led to the isolation of a total of 117 isolates. According to colony morphologies, and identification based upon 16S rRNA and gyrB gene sequence analysis, we obtained 58 taxonomically distinct strains. Depending on the similarity indexes, a gyrB sequence comparison appeared more useful than 16S rRNA sequence analysis for inferring intra- and some intergeneric relationships between the isolates.
Unearthing Antibiotic Resistance Associated with Disturbance-Induced Permafrost Thaw in Interior Alaska
Authors: Haan Tracie J. , Drown Devin M.
Published: January 2021

Monitoring antibiotic resistance genes (ARGs) across ecological niches is critical for assessing the impacts distinct microbial communities have on the global spread of resistance. In permafrost-associated soils, climate and human driven disturbances augment near-surface thaw shifting the predominant bacteria that shape the resistome in overlying active layer soils. This thaw is of concern in Alaska, because 85% of land is underlain by permafrost, making soils especially vulnerable to disturbances. The goal of this study is to assess how soil disturbance, and the subsequent shift in community composition, will affect the types, abundance, and mobility of ARGs that compose the active layer resistome. We address this goal through the following aims: (1) assess resistance phenotypes through antibiotic susceptibility testing, and (2) analyze types, abundance, and mobility of ARGs through whole genome analyses of bacteria isolated from a disturbance-induced thaw gradient in Interior Alaska. We found a high proportion of isolates resistant to at least one of the antibiotics tested with the highest prevalence of resistance to ampicillin. The abundance of ARGs and proportion of resistant isolates increased with disturbance; however, the number of ARGs per isolate was explained more by phylogeny than isolation site. When compared to a global database of soil bacteria, RefSoil+, our isolates from the same genera had distinct ARGs with a higher proportion on plasmids. These results emphasize the hypothesis that both phylogeny and ecology shape the resistome and suggest that a shift in community composition as a result of disturbance-induced thaw will be reflected in the predominant ARGs comprising the active layer resistome.
Higher Sensitivity of Soil Microbial Network Than Community Structure under Acid Rain
Authors: Liu Ziqiang , Wei Hui , Zhang Jiaen , Saleem Muhammad , He Yanan , Zhong Jiawen , Ma Rui
Published: January 2021

Acid rain (AR), as a global environmental threat, has profoundly adverse effects on natural soil ecosystems. Microorganisms involved in the nitrogen (N) cycle regulate the global N balance and climate stabilization, but little is known whether and how AR influences the structure and complexity of these microbial communities. Herein, we conducted an intact soil core experiment by manipulating the acidity of simulated rain (pH 7.5 (control, CK) vs. pH 4.0 (AR)) in subtropical agricultural soil, to reveal the differences in the structure and complexity of soil nitrifying and denitrifying microbiota using Illumina amplicon sequencing of functional genes (amoA, nirS, and nosZ). Networks of ammonia-oxidizing archaea (AOA) and nirS-carrying denitrifiers in AR treatment were less complex with fewer nodes and lower connectivity, while network of nosZ-carrying denitrifiers in AR treatment had higher complexity and connectivity relative to CK. Supporting this, AR reduced the abundance of keystone taxa in networks of AOA and nirS-carrying denitrifiers, but increased the abundance of keystone taxa in nosZ-carrying denitrifiers network. However, AR did not alter the community structure of AOA, ammonia-oxidizing bacteria (AOB), nirS-, and nosZ-carrying denitrifiers. Moreover, AR did not change soil N2O emissions during the experimental period. AOB community structure significantly correlated with content of soil available phosphorus (P), while the community structures of nirS- and nosZ-carrying denitrifiers both correlated with soil pH and available P content. Soil N2O emission was mainly driven by the nirS-carrying denitrifiers. Our results present new perspective on the impacts of AR on soil N-cycle microbial network complexity and keystone taxa in the context of global changes.
A Review of Composite Phase Change Materials Based on Porous Silica Nanomaterials for Latent Heat Storage Applications
Authors: Mitran Raul-Augustin , Ioniţǎ Simona , Lincu Daniel , Berger Daniela , Matei Cristian
Published: January 2021

Phase change materials (PCMs) can store thermal energy as latent heat through phase transitions. PCMs using the solid-liquid phase transition offer high 100–300 J g−1 enthalpy at constant temperature. However, pure compounds suffer from leakage, incongruent melting and crystallization, phase separation, and supercooling, which limit their heat storage capacity and reliability during multiple heating-cooling cycles. An appropriate approach to mitigating these drawbacks is the construction of composites as shape-stabilized phase change materials which retain their macroscopic solid shape even at temperatures above the melting point of the active heat storage compound. Shape-stabilized materials can be obtained by PCMs impregnation into porous matrices. Porous silica nanomaterials are promising matrices due to their high porosity and adsorption capacity, chemical and thermal stability and possibility of changing their structure through chemical synthesis. This review offers a first in-depth look at the various methods for obtaining composite PCMs using porous silica nanomaterials, their properties, and applications. The synthesis and properties of porous silica composites are presented based on the main classes of compounds which can act as heat storage materials (paraffins, fatty acids, polymers, small organic molecules, hydrated salts, molten salts and metals). The physico-chemical phenomena arising from the nanoconfinement of phase change materials into the silica pores are discussed from both theoretical and practical standpoints. The lessons learned so far in designing efficient composite PCMs using porous silica matrices are presented, as well as the future perspectives on improving the heat storage materials.
Antibacterial Activity and Mechanism of Linalool against Shewanella putrefaciens
Authors: Guo Fengyu , Liang Qiong , Zhang Ming , Chen Wenxue , Chen Haiming , Yun Yonghuan , Zhong Qiuping , Chen Weijun
Published: January 2021

The demand for reduced chemical preservative usage is currently growing, and natural preservatives are being developed to protect seafood. With its excellent antibacterial properties, linalool has been utilized widely in industries. However, its antibacterial mechanisms remain poorly studied. Here, untargeted metabolomics was applied to explore the mechanism of Shewanella putrefaciens cells treated with linalool. Results showed that linalool exhibited remarkable antibacterial activity against S. putrefaciens, with 1.5 µL/mL minimum inhibitory concentration (MIC). The growth of S. putrefaciens was suppressed completely at 1/2 MIC and 1 MIC levels. Linalool treatment reduced the membrane potential (MP); caused the leakage of alkaline phosphatase (AKP); and released the DNA, RNA, and proteins of S. putrefaciens, thus destroying the cell structure and expelling the cytoplasmic content. A total of 170 differential metabolites (DMs) were screened using metabolomics analysis, among which 81 species were upregulated and 89 species were downregulated after linalool treatment. These DMs are closely related to the tricarboxylic acid (TCA) cycle, glycolysis, amino acid metabolism, pantothenate and CoA biosynthesis, aminoacyl-tRNA biosynthesis, and glycerophospholipid metabolism. In addition, linalool substantially affected the activity of key enzymes, such as succinate dehydrogenase (SDH), pyruvate kinase (PK), ATPase, and respiratory chain dehydrogenase. The results provided some insights into the antibacterial mechanism of linalool against S. putrefaciens and are important for the development and application of linalool in seafood preservation.
Anti-Inflammatory Activity and Mechanism of Isookanin, Isolated by Bioassay-Guided Fractionation from Bidens pilosa L.
Authors: Xin Ying-Ji , Choi Soojung , Roh Kyung-Baeg , Cho Eunae , Ji Hyanggi , Weon Jin Bae , Park Deokhoon , Whang Wan Kyunn , Jung Eunsun
Published: January 2021

Bidens pilosa L. (Asteraceae) has been used historically in traditional Asian medicine and is known to have a variety of biological effects. However, the specific active compounds responsible for the individual pharmacological effects of Bidens pilosa L. (B. pilosa) extract have not yet been made clear. This study aimed to investigate the anti-inflammatory phytochemicals obtained from B. pilosa. We isolated a flavonoids-type phytochemical, isookanin, from B. pilosa through bioassay-guided fractionation based on its capacity to inhibit inflammation. Some of isookanin’s biological properties have been reported; however, the anti-inflammatory mechanism of isookanin has not yet been studied. In the present study, we evaluated the anti-inflammatory activities of isookanin using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We have shown that isookanin reduces the production of proinflammatory mediators (nitric oxide, prostaglandin E2) by inhibiting the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated macrophages. Isookanin also inhibited the expression of activator protein 1 (AP-1) and downregulated the LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-jun NH2-terminal kinase (JNK) in the MAPK signaling pathway. Additionally, isookanin inhibited proinflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-1β (IL-1β)) in LPS-induced THP-1 cells. These results demonstrate that isookanin could be a potential therapeutic candidate for inflammatory disease.
Structural Characterization of Act c 10.0101 and Pun g 1.0101—Allergens from the Non-Specific Lipid Transfer Protein Family
Authors: O’Malley Andrea , Pote Swanandi , Giangrieco Ivana , Tuppo Lisa , Gawlicka-Chruszcz Anna , Kowal Krzysztof , Ciardiello Maria Antonietta , Chruszcz Maksymilian
Published: January 2021

(1) Background: Non-specific lipid transfer proteins (nsLTPs), which belong to the prolamin superfamily, are potent allergens. While the biological role of LTPs is still not well understood, it is known that these proteins bind lipids. Allergen nsLTPs are characterized by significant stability and resistance to digestion. (2) Methods: nsLTPs from gold kiwifruit (Act c 10.0101) and pomegranate (Pun g 1.0101) were isolated from their natural sources and structurally characterized using X-ray crystallography (3) Results: Both proteins crystallized and their crystal structures were determined. The proteins have a very similar overall fold with characteristic compact, mainly α-helical structures. The C-terminal sequence of Act c 10.0101 was updated based on our structural and mass spectrometry analysis. Information on proteins’ sequences and structures was used to estimate the risk of cross-reactive reactions between Act c 10.0101 or Pun g 1.0101 and other allergens from this family of proteins. (4) Conclusions: Structural studies indicate a conformational flexibility of allergens from the nsLTP family and suggest that immunoglobulin E binding to some surface regions of these allergens may depend on ligand binding. Both Act c 10.0101 and Pun g 1.0101 are likely to be involved in cross-reactive reactions involving other proteins from the nsLTP family.
Preparation of High-Performance Activated Carbon from Coffee Grounds after Extraction of Bio-Oil
Authors: Ren Jie , Chen Nanwei , Wan Li , Li Guojian , Chen Tao , Yang Fan , Sun Shuiyu
Published: January 2021

In this study, a new method for economical utilization of coffee grounds was developed and tested. The resulting materials were characterized by proximate and elemental analyses, thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and N2 adsorption–desorption at 77 K. The experimental data show bio-oil yields reaching 42.3%. The optimal activated carbon was obtained under vacuum pyrolysis self-activation at an operating temperature of 450 °C, an activation temperature of 600 °C, an activation time of 30 min, and an impregnation ratio with phosphoric acid of 150 wt.%. Under these conditions, the yield of activated carbon reached 27.4% with a BET surface area of 1420 m2·g−1, an average pore size of 2.1 nm, a total pore volume of 0.747 cm3·g−1, and a t-Plot micropore volume of 0.428 cm3·g−1. In addition, the surface of activated carbon looked relatively rough, containing mesopores and micropores with large amounts of corrosion pits.
The Influence of the Structural and Morphological Properties of WO3 Thin Films Obtained by PLD on the Photoelectrochemical Water-Splitting Reaction Efficiency
Authors: Andrei Florin , Andrei Andreea , Birjega Ruxandra , Sirjita Eduard Nicolae , Radu Alina Irina , Dinescu Maria , Ion Valentin , Maraloiu Valentin-Adrian , Teodorescu Valentin Şerban , Scarisoreanu Nicu Doinel
Published: January 2021

Due to its physical and chemical properties, the n-type tungsten oxide (WO3) semiconductor is a suitable photoanode for water decomposition reaction. The responses of the photoelectrochemical PEC water-splitting properties as an effect of structural and optical changes of WO3 thin films, as well as the nature of electrolyte solutions, were studied in this work. The WO3 thins films have been obtained by pulsed laser deposition (PLD) on silicon (Si(001)) covered with platinum substrates using three different laser wavelengths. As the XRD (X-ray diffraction) and XTEM (cross-section transmission electron microscopy) analysis shows, the formation of highly crystalline monocline WO3 phase is formed for the film deposited at 1064 nm wavelength and poor crystalline phases with a large ordering anisotropy, characteristic of 2D structures for the films deposited at 355 nm and 193 nm wavelengths, respectively. The photogenerated current densities Jph depend on the laser wavelength, in both alkaline and acidic electrolyte. The maximum values of the photocurrent density have been obtained for the sample prepared with laser emitting at 355 nm. This behavior can be correlated with the coherent crystallized atomic ordering that appear for long distances (10–15 nm) in the (001) plane of the monoclinic WO3 phase structure films obtained at 355 nm laser wavelength. All the samples show poor current density in dark conditions and they are very stable in both acidic and alkaline solutions. The highest photocurrent density value is obtained in acidic solution for the WO3 thin film prepared by 355 nm laser (29 mA/cm2 at 1.6 V vs. RHE (1.35 V vs. Ag/AgCl)).
Regulation of Postabsorptive and Postprandial Glucose Metabolism by Insulin-Dependent and Insulin-Independent Mechanisms: An Integrative Approach
Authors: Dimitriadis George D. , Maratou Eirini , Kountouri Aikaterini , Board Mary , Lambadiari Vaia
Published: January 2021

Glucose levels in blood must be constantly maintained within a tight physiological range to sustain anabolism. Insulin regulates glucose homeostasis via its effects on glucose production from the liver and kidneys and glucose disposal in peripheral tissues (mainly skeletal muscle). Blood levels of glucose are regulated simultaneously by insulin-mediated rates of glucose production from the liver (and kidneys) and removal from muscle; adipose tissue is a key partner in this scenario, providing nonesterified fatty acids (NEFA) as an alternative fuel for skeletal muscle and liver when blood glucose levels are depleted. During sleep at night, the gradual development of insulin resistance, due to growth hormone and cortisol surges, ensures that blood glucose levels will be maintained within normal levels by: (a) switching from glucose to NEFA oxidation in muscle; (b) modulating glucose production from the liver/kidneys. After meals, several mechanisms (sequence/composition of meals, gastric emptying/intestinal glucose absorption, gastrointestinal hormones, hyperglycemia mass action effects, insulin/glucagon secretion/action, de novo lipogenesis and glucose disposal) operate in concert for optimal regulation of postprandial glucose fluctuations. The contribution of the liver in postprandial glucose homeostasis is critical. The liver is preferentially used to dispose over 50% of the ingested glucose and restrict the acute increases of glucose and insulin in the bloodstream after meals, thus protecting the circulation and tissues from the adverse effects of marked hyperglycemia and hyperinsulinemia.
Harbour Seals: Population Structure, Status, and Threats in a Rapidly Changing Environment
Authors: Blanchet Marie-Anne , Vincent Cécile , Womble Jamie N. , Steingass Sheanna M. , Desportes Geneviève
Published: January 2021

The harbour seal (Phoca vitulina) is the world’s most widely distributed pinniped species ranging from temperate to Arctic regions (30–78.5° N in the Atlantic, 28–61.2° N in the Pacific), but no detailed overview of the species status exists. The aims of this review are to (i) provide current information on the genetic structure, population status, and threats; (ii) review potential consequences of a changing climate; and (iii) identify knowledge gaps to guide future research and monitoring. Although the species is globally abundant, wide differences exist across the species’ broad range. As climate warms, populations at the edges of the species’ distributional range are likely to be more affected. The primary climate-related drivers include: (i) changes in weather patterns, which can affect thermoregulation; (ii) decrease in availability of haul-out substrates; (iii) large-scale changes in prey availability and inter-specific competition; (iv) shifts in the range of pathogens; (v) increase in temperature favouring the biotransformation of contaminants; and (vi) increased exposure to pollutant from increased freshwater run-off. Multiple anthropogenic stressors may collectively impact some populations. Coordinated monitoring efforts across and within regions is needed. This would allow for a spatially explicit management approach including population-specific responses to known stressors.
Development and Evaluation of a Web-Based Paediatric Drug Information System for Germany
Authors: Zahn Julia , Wimmer Stefan , Rödle Wolfgang , Toni Irmgard , Sedlmayr Brita , Prokosch Hans-Ulrich , Rascher Wolfgang , Neubert Antje
Published: January 2021

Background: Off-label use is frequent in paediatrics but that does not necessarily mean that the risk-benefit ratio is negative. Nevertheless, evidence-based data is essential for safe drug therapy. In Germany, there is no publicly available compendium providing transparent, evidence-based information for paediatric pharmacotherapy to date. This work describes the development of a web-based paediatric drug information system (PDIS) for Germany and its evaluation by health care professionals (HCP). Methods: Since 2012, a PDIS is being developed by the authors and is supported by the Federal Ministry of Health since 2016. Dosing recommendations were established based on systematic literature reviews and subsequent evaluation by clinical experts. The prototype was evaluated by HCP. Based on the results, the further development was concluded. Results: 92% of HCP believed that the PDIS could improve the quality of prescribing, as currently available information is deficient. Besides the license and formulations, dosing recommendations were the most relevant modules. A dosage calculator was the most wanted improvement. To facilitate sustainability of future development, a collaboration with the Dutch Kinderformularium was established. As of 2021, the database will be available to German HCP. Conclusion: The fundamentals for a German PDIS were established, and vital steps were taken towards successful continuation.
Effectiveness of Music Interventions to Reduce Test Anxiety in Pharmacy Students
Authors: Galal Suzanne , Vyas Deepti , Hackett Rachelle Kisst , Rogan Ed , Nguyen Chloe
Published: January 2021

Background: The main objective of this pilot study was to evaluate the impact of a classroom activity involving music on anxiety associated with preparing for and taking an assessment. Methods: Two hundred and two (202) pharmacy students were randomly assigned to one of two conditions of the experimental study: active music playing (n = 103) versus passive music listening (n = 99). All students completed a pre-test, a mid-test, and a post-test including: an “Attitudes and Perceptions” survey, State-Trait Anxiety Inventory for Adults (STAI Survey), and a knowledge assessment. Data were analyzed to determine the impact each of the music interventions had on students’ test anxiety. Results: Both types of musical interventions produced similar results in terms of anxiety reduction. Faced with an upcoming test prior to the musical intervention, average state-trait anxiety scores increased; after the musical intervention, state-trait anxiety scores decreased. Conclusions: The use of music helped to reduce test anxiety, even after one brief musical intervention, regardless of whether students passively listened to music or actively played music.
Impact of a Medication Adherence Packaging Service on Patient-Centered Outcomes at an Independent Community Pharmacy
Authors: Phi Catherine , Berenbrok Lucas A. , Carroll Joni C. , Firm Ashley , McGivney Melissa Somma , Coley Kim C.
Published: January 2021

The purpose of this project was to evaluate the impact of a comprehensive medication adherence packaging (RxMAP) service on patient medication-taking behaviors and patient-centered outcomes. Adult patients who utilized a single independent community pharmacy, enrolled in the RxMAP service for at least two consecutive cycles, and managed their own medications were eligible. The RxMAP service consists of multi-dose blister packaging in 28-day cycles, medication synchronization, monthly touchpoint calls, and delivery/mailing. A 13-item telephonic survey was administered, and patients’ verbal responses were captured by audio-recording and detailed note taking. Descriptive statistics were used to quantify the results and illustrative quotes representing the interview domains were selected. There were 42 patients who completed the survey: 88% reported they missed fewer doses compared to before using RxMAP; 71% were more likely to take their medications on time each day; 86% were more confident with managing their medications; and 74% were more independent. Finally, 64% of patients stated their overall quality of life was better now compared to before using the packaging service. These results demonstrate that medication adherence packaging services can positively impact patients’ medication-taking behaviors, increase their confidence in medication management, and improve perceived quality of life.
Attending a Blended In-Service Management Training in a Public Health System: Constraints and Opportunities for Pharmacists and Health Services
Authors: Lorenzoni Andrigo Antonio , Manzini Fernanda , da Trindade Monica Cristina Nunes , Storb Bernd Heinrich , Rech Norberto , Farias Mareni Rocha , Leite Silvana Nair
Published: January 2021

Management and public health are important domains of competency for pharmacists. In about 90% of Brazilian health departments, pharmacists manage the selection and purchase of medicines. The Pharmaceutical Services and Access to Medicines Management Course (PSAMM) was offered to pharmacists working in the public health system. The aim of this study is to analyze the impacts of the course as perceived by the students (pharmacists). Two thousand five hundred pharmacists completed the course. It is a mixed-methods study, including subscribing forms (n = 2500), evaluation questionnaire (n = 1500), focus groups (n = 10), and semi-structured interviews (n = 31). Participants reported a high level of satisfaction with the course; they considered to have developed competencies related to leadership and management, competencies needed to enhance and sustain their practices in health services. Data analyses showed important barriers to complete the course: high course workload, poor quality of Internet access, lack of support from the health services. Participants highlighted crucial features of the course that helped them develop key competencies: practical in-service activities, useful and realistic contents, tutoring. These features helped participants overcome some important constraints described by them. The educational model described in this study was perceived as having a long-term impact on their behaviors and management practices in health services.
Next Chapter in the Legend of Silphion: Preliminary Morphological, Chemical, Biological and Pharmacological Evaluations, Initial Conservation Studies, and Reassessment of the Regional Extinction Event
Authors: Miski Mahmut
Published: January 2021

Silphion was an ancient medicinal gum-resin; most likely obtained from a Ferula species growing in the Cyrene region of Libya ca. 2500 years ago. Due to its therapeutic properties and culinary value, silphion became the main economic commodity of the Cyrene region. It is generally believed that the source of silphion became extinct in the first century AD. However, there are a few references in the literature about the cultivated silphion plant and its existence up to the fifth century. Recently, a rare and endemic Ferula species that produces a pleasant-smelling gum-resin was found in three locations near formerly Greek villages in Anatolia. Morphologic features of this species closely resemble silphion, as it appears in the numismatic figures of antique Cyrenaic coins, and conform to descriptions by ancient authors. Initial chemical and pharmacological investigations of this species have confirmed the medicinal and spice-like quality of its gum-resin supporting a connection with the long-lost silphion. A preliminary conservation study has been initiated at the growth site of this rare endemic Ferula species. The results of this study and their implications on the regional extinction event, and future development of this species will be discussed.
Metabolome Analysis Revealed the Mechanism of Exogenous Glutathione to Alleviate Cadmium Stress in Maize (Zea mays L.) Seedlings
Authors: Wang Runfeng , Lin Kaina , Chen Huabin , Qi Zhenyu , Liu Bohan , Cao Fangbin , Chen Hao , Wu Feibo
Published: January 2021

Cadmium (Cd) is one of the major heavy metal pollutants in the environment and imposes severe limitations on crop growth and production. Glutathione (GSH) plays an important role in plant Cd tolerance which is able to scavenge stresses-induced reactive oxygen species (ROS) and is involved in the biosynthesis of phytochelatins (PCs). Our previous study revealed that Cd stress affects maize growth, and the GSH treatment could relieve Cd stress in maize seedlings. In this study, we attempted to characterize the metabolomics changes in maize leaves and roots under Cd stress and exogenous GSH conditions. We identified 145 and 133 metabolites in the leaves and roots, respectively. Cd stress decreased the tricarboxylic acid cycle (TCA cycle) metabolism and increased the amino acid contents in the leaves, while it decreased the amino acid contents, increased the TCA cycle metabolism, the sugar contents, and shikimic acid metabolism in the roots. On the other hand, exogenous GSH increased the GSH content, changed the production of metabolites related to antioxidant systems (such as ascorbic acid-related metabolites and flavonoid-related metabolites), and alleviated lipid peroxidation, thereby alleviating the toxic effect of Cd stress on maize. These findings support the idea that GSH alleviates Cd-induced stress in maize and may help to elucidate the mechanism governing Cd-induced stress and the GSH-driven alleviation effect.
Preparation, Properties and Mechanisms of Carbon Fiber/Polymer Composites for Thermal Management Applications
Authors: Ali Zulfiqar , Gao Yuan , Tang Bo , Wu Xinfeng , Wang Ying , Li Maohua , Hou Xiao , Li Linhong , Jiang Nan , Yu Jinhong
Published: January 2021

With the increasing integration and miniaturization of electronic devices, heat dissipation has become a major challenge. The traditional printed polymer circuit board can no longer meet the heat dissipation demands of microelectronic equipment. If the heat cannot be removed quickly and effectively, the efficiency of the devices will be decreased and their lifetime will be shortened. In addition, the development of the aerospace, automobiles, light emitting diode (LED{ TA \1 “LED; lightemitting diode” \s “LED” \c 1 }) and energy harvesting and conversion has gradually increased the demand for low-density and high thermal conductive materials. In recent years, carbon fiber (CF{ TA \1 “CF; carbon fiber” \c 1 }) has been widely used for the preparation of polymer composites due to its good mechanical property and ultra-high thermal conductivity. CF materials easily form thermal conduction paths through polymer composites to improve the thermal conductivity. This paper describes the research progress, thermal conductivity mechanisms, preparation methods, factors influencing thermal conductivity and provides relevant suggestions for the development of CF composites for thermal management.
Sensors for Structural Health Monitoring of Agricultural Structures
Authors: Maraveas Chrysanthos , Bartzanas Thomas
Published: January 2021

The health diagnosis of agricultural structures is critical to detecting damages such as cracks in concrete, corrosion, spalling, and delamination. Agricultural structures are susceptible to environmental degradation due to frequent exposure to water, organic effluent, farm chemicals, structural loading, and unloading. Various sensors have been employed for accurate and real-time monitoring of agricultural building structures, including electrochemical, ultrasonic, fiber-optic, piezoelectric, wireless, fiber Bragg grating sensors, and self-sensing concrete. The cost–benefits of each type of sensor and utility in a farm environment are explored in the review. Current literature suggests that the functionality of sensors has improved with progress in technology. Notable improvements made with the progress in technology include better accuracy of the measurements, reduction of signal-to-noise ratio, and transmission speed, and the deployment of machine learning, deep learning, and artificial intelligence in smart IoT-based agriculture. Key challenges include inconsistent installation of sensors in farm structures, technical constraints, and lack of support infrastructure, awareness, and preference for traditional inspection methods.
Do Work–Life Measures Really Matter? The Impact of Flexible Working Hours and Home-Based Teleworking in Preventing Voluntary Employee Exits
Authors: Marx Charlotte K. , Reimann Mareike , Diewald Martin
Published: January 2021

Numerous studies have demonstrated the importance of work–life measures, which are designed to contribute to job quality and help reconcile employees’ work and personal lives. In our study, we asked whether such measures can also work as inducements to prevent employees from voluntarily leaving a firm. We considered flexible working hours and home-based teleworking as flexibility measures that are potentially attractive to all employees. To address the possible bias caused by sketchy implementation and their actual selective use, we chose to examine employees’ perceptions of the offer of these measures. We investigated the moderation of the effect by organizational culture and supervisor and coworker support. We controlled for several indicators of job quality, such as job satisfaction and perceived fairness, to isolate specific ways in which work–life measures contributed to voluntary employee exit, and checked for a selective attractiveness of work–life measures to parents and women as the main caregivers. Using a three-wave panel employer–employee survey, we estimated multilevel mixed-effects logistic regression models for 5452 employees at 127 large German establishments. Our results confirmed that both types of flexibility measures were associated with a lower probability of voluntarily exit. This applied more to men than to women, and the probability was reduced by a demanding organizational culture. Both measures seemed not to be specifically designed to accommodate main caregivers but were attractive to the whole workforce.
International Trade in Transport Services between Poland and the European Union
Authors: Malkowska Agnieszka , Malkowski Arkadiusz
Published: January 2021

The structure of international trade in today’s global economy is undergoing transformation. The increase in the importance of services is a clear trend. In Polish foreign trade with the European Union countries, it is transport services that to the greatest extent create the trade surplus. These services significantly affect the environment, which makes it necessary to take measures aimed at the implementation of the green transport principles. The aim of the study was to provide a detailed description of transport services in Polish foreign trade with European Union (EU) countries and to assess the role of these services in Polish economy, including in terms of sustainable development. The study concerned transport services, consisting of four separate types: maritime transport, air transport, other transport services (except maritime and air) and postal and courier services. The time span of the study was 2010–2018. The latest secondary data from the official statistics of the National Bank of Poland and the Central Statistical Office were used for the purpose of the analysis. The research methods included: critical analysis of the source literature, analysis of secondary data (desk-research) and graphic methods (tables and diagrams), by means of which the results of the study were presented. The conclusions drawn are inductive and deductive in nature. The article describes in detail Polish trade in transport services with EU countries. It focuses on export, import and balance. The role of the examined services in foreign trade and Polish economy was assessed based on the analysis of the structure of export and import and the rate of export and import, geographical orientation of trade, as well as the activity of enterprises from the transport service sector, registered in Poland, including joint ventures. Research has shown that between 2010 and 2018 Polish export and import of transport services as part of trade relations with EU countries increased considerably. The conducted research indicates that transport services are a crucial element of Polish trade. The role of EU countries in the Polish trade in transport services was recognised as significant and growing. This has a positive impact on the country’s economy. The increasing role of road transport in the structure of the Polish trade indicates that Polish entities providing transport services are thriving in this demanding market. However, the analysis of the source literature indicated changes in the preferences of consumers, who increasingly often expect the implementation of sustainable development principles in the distribution of goods. Green marketing or green transport are concepts more and more frequently used to gain competitive advantage in the market. The transport sector in Poland is facing a major trend towards the implementation of sustainable development principles. In order to maintain sales growth internationally, the entities will have to implement sustainable development principles.
Influence of Design Parameters of Idler Bearing Units on the Energy Consumption of a Belt Conveyor
Authors: Kulinowski Piotr , Kasza Piotr , Zarzycki Jacek
Published: January 2021

This publication presents the results of laboratory tests of idler rolling resistance under operational loads. Operational loads are understood as radial and axial forces acting on the idler, with values corresponding to those that occur in the conditions of its operation in copper ore mines. Knowing the rolling resistance is important not only at the stage of conveyor design, selection of the drive power or calculations of the necessary belt strength, but also when improving and searching for new idler design solutions. The idlers adopted for this research were differentiated in terms of bearings and idler axial clearance. The investigations were carried out on a unique test stand designed and built by the authors. The construction of the stand enables simulating operational loads while measuring the rolling resistance. The test rig measures idler bearing losses and rolling drag, not belt indentation rolling resistance. The object of the research were ø133×465 idlers, which are most commonly used in the raw materials industry. The results show the possibility of reducing the belt conveyor energy consumption by appropriate selection of the design features of the idler bearing unit.
Promoting Reviewer-Related Attribution: Moderately Complex Presentation of Mixed Opinions Activates the Analytic Process
Authors: Xie Guangming , Du Wenbo , Yuan Hongping , Jiang Yushi
Published: January 2021

Using metacognition and dual process theories, this paper studied the role of types of presentation of mixed opinions in mitigating negative impacts of online word of mouth (WOM) dispersion on consumer’s purchasing decisions. Two studies were implemented, respectively. By employing an eye-tracking approach, study 1 recorded consumer’s attention to WOM dispersion. The results show that the activation of the analytic system can improve reviewer-related attribution options. In study 2, three kinds of presentation of mixed opinions originating from China’s leading online platform were compared. The results demonstrated that mixed opinions expressed in moderately complex form, integrating average ratings and reviewers’ impressions of products, was effective in promoting reviewer-related attribution choices. However, too-complicated presentation types of WOM dispersion can impose excessively on consumers’ cognitive load and eventually fail to activate the analytic system for promoting reviewer-related attribution choices. The main contribution of this paper lies in that consumer attribution-related choices are supplemented, which provides new insights into information consistency in consumer research. The managerial and theoretical significance of this paper are discussed in order to better understand the purchasing decisions of consumers.
A importância dos movimentos em massa na dinâmica de vertentes no triângulo Mineiro/Alto Paranaíba: o emprego da análise multifatorial para a sua compreensão. Ensaio metodológico
Authors:   Universidade Federal de Uberlândia, Bombonatto Danelon Jean Roger , Sousa Pedrosa António de ,   Universidade Federal de Uberlândia
Published: January 2021

A aplicação do método de análise multifatorial para a determinação da susceptibilidade aos movimentos em massa no Triângulo Mineiro/Alto Paranaíba tem como objetivo principal a sua espacialização e também a compreensão dos fatores mais influentes no seu desencadeamento. Para além de se compreender a sua distribuição espacial, o resultado do modelo pode ser importante em termos de ordenamento do território, pois permite a adoção de medidas de prevenção e mitigação para evitar o seu desencadeamento e, ao mesmo tempo, para minimizar as suas consequências. Para aplicação de tal ensaio foram utilizadas técnicas de geoprocessamento em ambiente SIG (Sistema de Informação Geográfica. Dentre os vários materiais cartográficos gerados pelo estudo, se destaca o mapa de Susceptibilidade aos Movimentos em Massa no Triângulo Minero/Alto Paranaíba, que espacializa as informações apresentadas no trabalho à respeito do tema movimentos em massa.
Percepção de riscos socioambientais em comunidades pesqueiras: uma análise no litoral da Bahia, Brasil
Authors:   Universidade Estadual de Feira de Santana, Vieira Serbeto Erika Silva , Ramos Almeida Raiza Rabelo ,   Universidade Estadual de Feira de Santana, de Ferreira Bandeira Fábio Pedro S. ,   Universidade Estadual de Feira de Santana
Published: January 2021

A Baía de Todos Santos (BTS) abrange comunidades ribeirinhas que são impactadas pela forte atividade industrial e petroquímica na região, ao tempo que existe uma carência de estudos que abordem a percepção de riscos socioambientais dessas comunidades. Objetivou-se analisar as percepções de risco socioambientais nas comunidades pesqueiras de Madre de Deus. No desenvolvimento, buscou-se um reconhecimento prévio da localidade e estabelecimento de contatos, após isso, conseguiu-se realizar oficinas onde a comunidade pôde listar os riscos socioambientais percebidos, bem como, sua gravidade e confeccionar uma linha do tempo. Foram citados 15 riscos. A maioria dos riscos (93,33%) foram classificados como muito graves. Na linha do tempo foram evidenciados elementos relevantes como a instalação da Petrobras no município, a carteira de pesca e o defeso. Os resultados apontam que a percepção de riscos socioambientais deve ser considerada em planos de gerenciamento. Faz-se necessária a compensação de danos por parte da indústria petrolífera para com essas comunidades e que o Estado cumpra seu papel de garantir os direitos socioambientais desses pescadores(as).