bluuebunny/crossref_metadata_2025 huggingface dataset:
A Case Report on Dysgraphia in a Patient Receiving Blinatumomab: Complex Characters Are Easy to Find in a Handwriting Test
Authors: Yamamoto Yasuto , Shimasaki Takeo , Ishigaki Yasuhito , Fujimoto Shino , Takahashi Yoshimitsu , Kimura Shiori , Aijo Keiko , Takayanagi Mami , Mizuta Shuichi , Masauji Togen , Masaki Yasufumi
Published: May 2022

Recent advances in chemotherapy have led to the emergence of new types of anticancer agents. With these advances, cases of side effects that have not been witnessed in the past have emerged. The systems of side effect evaluation and their grading have been based on the existing knowledge, such as the CTCAE (Common Terminology Standard for Adverse Events) for evaluating adverse drug reactions in cancer chemotherapy clinical trials. Therefore, new types of side effects may be overlooked or underestimated. Blinatumomab is a bispecific T-cell–engager (BiTE) antibody with specificity for CD19 on B cells and CD3 on T cells. Neurological events, such as neuropathy and encephalopathy, are serious side effects of BiTE antibodies. We encountered a case of a 62-year-old woman who experienced short-term memory impairment and dysgraphia after the first blinatumomab administration for Philadelphia chromosome negative (Ph−) B-cell acute lymphoblastic leukemia (ALL). The CTCAE does not include dysgraphia as a classifier for antibody therapies, such as blinatumomab, and immune effector cell-associated neurotoxicity syndrome, which is defined as a Chimeric antigen receptor T cell therapy-related toxicity; dysgraphia is included in the list of symptoms but is not graded. In this case, the severity of dysgraphia differed depending on the complexity of the letters examined. There is no report that the severity of dysgraphia depends on the letters’ complexity, and therefore, it may be overlooked when using simple letters. We have reported the characteristics of dysgraphia in this case and the differences observed when judging different letters.
Clinical Manifestations and Laboratory Findings of Kawasaki Disease: Beyond the Classic Diagnostic Features
Authors: Lee Wendy , Cheah Chooi San , Suhaini Siti Aisyah , Azidin Abdullah Harith , Khoo Mohammad Shukri , Ismail Noor Akmal Shareela , Ali Adli
Published: May 2022

Kawasaki disease (KD) has shown a marked increase in trend over the globe, especially within the last two decades. Kawasaki disease is often seen in the paediatric population below five years old, while it is rare for those who are beyond that age. Up to this date, no exact causes has been identified although KD was found more than half a century ago. The underlying pathogenesis of the disease is still unelucidated, and researchers are trying to unlock the mystery of KD. To further complicate the diagnosis and the prompt management, a specific biomarker for the diagnosis of KD is yet to be discovered, making it hard to differentiate between KD and other diseases with a similar presentation. Nonetheless, since its discovery, clinicians and scientists alike had known more about the different clinical aspects of typical KD. Thus, this article intends to revisit and review the various clinical manifestations and laboratory characteristics of KD in order to guide the diagnosis of KD.
An Evaluation of Jaw Tracking Movements in Patients with Total Joint Replacements versus a Control Group
Authors: Rahman Farhana , Femiano Felice , Louis Patrick J , Kau Chung How
Published: May 2022

Background and Objectives: One form of treatment for degenerative temporomandibular joint diseases such as osteoarthritis, rheumatic arthritis, TMJ ankylosis, and condylar resorption is total joint replacement. The aim of this study was to examine the function of the temporomandibular joint after prosthetic joint replacement. Materials and methods: Fifteen patients with unilateral or bilateral TMJ total joint replacements and 15 healthy controls were evaluated via a SICAT JMT+ device. This non-invasive system measures 3D position and linear movements in all degrees of freedom and allows undisturbed functional mandibular movements to provide a quantitative evaluation. In addition, a TMJ questionnaire consisting of the subjective symptoms was also obtained. To date, no similar studies have been cited in the literature. Results: Mandibular movements after prosthetic joint replacement were recorded during opening, closing, protrusion, and lateral excursive movements and were all significantly decreased compared to those of controls. In the treatment group, the maximum incisal opening was 33.46 ± 5.47 mm, left lateral movement was 1.91 ± 2.7 mm, right lateral movement was 1.74 ± 1.74 mm, and protrusive movement was 2.83 ± 2.05 mm. The p-value comparison study and control group indicated significant difference (p < 0.0001) between the two groups. The study group stated a high level of satisfaction with the total joint replacement. Conclusion: Within the limitations of the study, the following conclusions can be drawn: (1) TMJ replacement patients showed significantly limited jaw movements compared to the control group; (2) a small percentage of TMJ replacement patients still present low levels of pain but improved chewing ability and quality of life.
Comment on Mankowska et al. Critical Flicker Fusion Frequency: A Narrative Review. Medicina 2021, 57, 1096
Authors: Vrijdag Xavier C. E. , van Waart Hanna , Sleigh Jamie W. , Mitchell Simon J.
Published: May 2022

We have read with great interest the review by Mankowska et al. [...]
Effect of Prehabilitation in Form of Exercise and/or Education in Patients Undergoing Total Hip Arthroplasty on Postoperative Outcomes—A Systematic Review
Authors: Widmer Patrick , Oesch Peter , Bachmann Stefan
Published: May 2022

Background and Objectives: The aim of this systematic review was to determine whether prehabilitation before total hip arthroplasty, in the form of exercise therapy, education alone, or both together, improves postoperative outcomes, such as physical functioning, compared with no intervention. Materials and Methods: A systematic literature search was performed in the online databases PubMed, PEDro and Cochrane Library using the following search keywords: “prehabilitation”, “preoperative care”, and “total hip replacement”. Results: A total of 400 potentially relevant studies were identified. After title, abstract and full-text screening, 14 studies fulfilled all inclusion criteria and were included in this systematic review. Patients who completed exercise-based prehabilitation before their operation showed significant postoperative improvements compared with no intervention in the following tests: six-minute walk test, Timed Up and Go test, chair-rise test, and stair climbing. For various other assessments, such as the widely used Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Hip disability and Osteoarthritis Outcome Score (HOOS), 36-item Short Form Survey (SF-36) and Barthel Index, no significant differences in outcomes regarding exercise therapy were reported in the included studies. Education alone had no effect on postoperative outcomes. Conclusions: Prehabilitation in the form of a prehabilitation exercise therapy is an effective prehabilitation measure with regard to postoperative physical functioning, while prehabilitation in the form of education has no significant effects. No negative effects of prehabilitation on the outcomes examined were reported.
Three-Dimensional Planning and Patient-Specific Instrumentation for the Fixation of Distal Radius Fractures
Authors: Pastor Tatjana , Nagy Ladislav , Fürnstahl Philipp , Roner Simon , Pastor Torsten , Schweizer Andreas
Published: May 2022

Background and Objectives: Three-dimensional planning and guided osteotomy utilizing patient-specific instrumentation (PSI) with the contralateral side used as a reference have been proven as effective in the treatment of malunions following complex fractures of the distal radius. However, this approach has not yet been described in relation to fracture reduction of the distal radius. The aim of this study was to assess the technical and logistical feasibility of computer-assisted surgery in a clinical setting using PSI for fracture reduction and fixation. Materials and Methods: Five patients with varied fracture patterns of the distal radius underwent operative treatment with using PSI. The first applied PSI guide allowed specific and accurate placement of Kirschner wires inside the multiple fragments, with subsequent concurrent reduction using a second guide. Results: Planning, printing of the guides, and operations were performed within 5.6 days on average (range of 1–10 days). All patients could be treated within a reasonable period of time, demonstrating good outcomes, and were able to return to work after a follow-up of three months. Mean wrist movements (°) were 58 (standard deviation (SD) 21) in flexion, 62 (SD 15) in extension, 73 (SD 4) in pronation and 74 (SD 10) in supination at a minimum follow-up of 6 months. Conclusions: Three-dimensional planned osteosynthesis using PSI for treatment of distal radius fractures is feasible and facilitates reduction of multiple fracture fragments. However, higher costs must be taken into consideration for this treatment.
Pectin Films with Recovered Sunflower Waxes Produced by Electrospraying
Authors: Chalapud Mayra C. , Baümler Erica R. , Carelli Amalia A. , Salgado-Cruz Ma. de la Paz , Morales-Sánchez Eduardo , Rentería-Ortega Minerva , Calderón-Domínguez Georgina
Published: May 2022

Valorization of by-products obtained from food processing has achieved an important environmental impact. In this research, sunflower wax recovered from oil refining process was incorporated to low and high-methoxyl pectin films produced by electrospraying. Film-forming solutions and wax-added electrosprayed films were physical and structurally evaluated. The addition of sunflower wax to the film-forming solutions reduces conductivity while raising surface tension and density, whereas the type of pectin had a larger impact on viscosity, with the low-methoxyl solution having the highest value. These changes in physical solution properties influenced the film characteristics, observing thicker films with lower water vapor transmission rate (WVTR) when adding wax. Micrographs obtained by scanning electron microscopy (SEM) revealed the presence of wax particles as small spherical shapes, having a good distribution through the sectional area of films. According to X-ray diffraction (XRD), atomic force microscopy (AFM) and mechanical properties analyses, the presence of wax had an impact on the degree of crystallinity, producing a more amorphous and rougher film’s structure, without affecting the elongation percentage and the tensile stress (p>0.05). These results showed that wax addition improves the physical properties of films, while the suitability of using both pectins and the electrospraying technique was demonstrated.
Biopolymer-Based Mixed Matrix Membranes (MMMs) for CO2/CH4 Separation: Experimental and Modeling Evaluation
Authors: Torre-Celeizabal Andrea , Casado-Coterillo Clara , Garea Aurora
Published: May 2022

Alternative materials are needed to tackle the sustainability of membrane fabrication in light of the circular economy, so that membrane technology keeps playing a role as sustainable technology in CO2 separation processes. In this work, chitosan (CS)-based mixed matrix thin layers have been coated onto commercial polyethersulfone (PES) supports. The CS matrix was loaded by non-toxic 1-Ethyl-3-methylimidazolium acetate ionic liquid (IL) and/or laminar nanoporous AM-4 and UZAR-S3 silicates prepared without costly organic surfactants to improve CO2 permselectivity and mechanical robustness. The CO2/CH4 separation behavior of these membranes was evaluated experimentally at different feed gas composition (CO2/CH4 feed mixture from 20:80 to 70:30%), covering different separation applications associated with this separation. A cross-flow membrane cell model built using Aspen Custom Modeler was used to validate the process performance and relate the membrane properties with the target objectives of CO2 and CH4 recovery and purity in the permeate and retentate streams, respectively. The purely organic IL-CS and mixed matrix AM-4:IL-CS composite membranes showed the most promising results in terms of CO2 and CH4 purity and recovery. This is correlated with their higher hydrophilicity and CO2 adsorption and lower swelling degree, i.e., mechanical robustness, than UZAR-S3 loaded composite membranes. The purity and recovery of the 10 wt.% AM-4:IL-CS/PES composite membrane were close or even surpassed those of the hydrophobic commercial membrane used as reference. This work provides scope for membranes fabricated from renewable or biodegradable polymers and non-toxic fillers that show at least comparable CO2/CH4 separation as existing membranes, as well as the simultaneous feedback on membrane development by the simultaneous correlation of the process requirements with the membrane properties to achieve those process targets.
Ibuprofen Removal by Graphene Oxide and Reduced Graphene Oxide Coated Polysulfone Nanofiltration Membranes
Authors: Hidalgo Asunción M. , Gómez María , Murcia María D. , León Gerardo , Miguel Beatriz , Gago Israel , Martínez Pilar M.
Published: May 2022

The presence of pharmaceutical products, and their metabolites, in wastewater has become a focus of growing environmental concern. Among these pharmaceutical products, ibuprofen (IBU) is one of the most consumed non-steroidal anti-inflammatory drugs and it can enter the environment though both human and animal consumption, because it is not entirely absorbed by the body, and the pharmaceutical industry wastewater. Nanofiltration has been described as an attractive process for the treatment of wastewater containing pharmaceutical products. In this paper, the modification of a polysulfone nanofiltration membrane by coating with graphene oxide (GO) and reduced graphene oxide (RGO) has been carried out. The morphology and elemental composition of the active layer of unmodified and modified membranes were analyzed by scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX), respectively. Initial characterization membranes was carried out, studying their water permeability coefficient and their permeate flux and rejection coefficients, at different applied pressures, using magnesium chloride solutions. The behavior of both pristine and coated membranes against ibuprofen solutions were analyzed by studying the permeate fluxes and the rejection coefficients at different pressures and at different contaminant concentrations. The results have shown that both GO and RGO coated membranes lead to higher values of ibuprofene rejection than that of uncoated membrane, the latter being the one that presents better results in the studies of permeability, selectivity, and fouling.
Evidence for a Physiological Role of T-Type Ca Channels in Ventricular Cardiomyocytes of Adult Mice
Authors: Marksteiner Jessica , Ebner Janine , Salzer Isabella , Lilliu Elena , Hackl Benjamin , Todt Hannes , Kubista Helmut , Hallström Seth , Koenig Xaver , Hilber Karlheinz
Published: May 2022

T-type Ca channels are strongly expressed and important in the developing heart. In the adult heart, these channels play a significant role in pacemaker tissues, but there is uncertainty about their presence and physiological relevance in the working myocardium. Here, we show that the T-type Ca channel isoforms Cav3.1 and Cav3.2 are expressed at a protein level in ventricular cardiomyocytes from healthy adult C57/BL6 mice. Myocytes isolated from adult wild-type and Cav3.2 KO mice showed considerable whole cell T-type Ca currents under beta-adrenergic stimulation with isoprenaline. We further show that the detectability of basal T-type Ca currents in murine wild-type cardiomyocytes depends on the applied experimental conditions. Together, these findings reveal the presence of functional T-type Ca channels in the membrane of ventricular myocytes. In addition, electrically evoked Ca release from the sarcoplasmic reticulum was significantly impaired in Cav3.2 KO compared to wild-type cardiomyocytes. Our work implies a physiological role of T-type Ca channels in the healthy adult murine ventricular working myocardium.
Conversion Kinetics and Ionic Conductivity in Na-β”-Alumina + YSZ (Naβ”AY) Sodium Solid Electrolyte via Vapor Phase Conversion Process
Authors: Zhu Liangzhu , Virkar Anil V.
Published: May 2022

Sodium ion batteries have been receiving increasing attention and may see potential revival in the near future, particularly in large-scale grid energy storage coupling with wind and solar power generation, due to the abundant sodium resources, low cost, and sufficiently high energy density. Among the known sodium ion conductors, the Na-β”-alumina electrolyte remains highly attractive because of its high ionic conductivity. This study focuses on the vapor phase synthesis of a Na-β”-Alumina + YSZ (Naβ”AY) composite sodium electrolyte, which has higher mechanical strength and stability than conventional single phase β”-Alumina. The objectives are the measurement of conversion kinetics through a newly developed weight-gain based model and the determination of sodium ionic conductivity in the composite electrolyte. Starting samples contained ~70 vol% α-Alumina and ~30 vol% YSZ (3 mol% Y2O3 stabilized Zirconia) with and without a thin alumina surface layer made by sintering in air at 1600 °C. The sintered samples were placed in a powder of Na-β”-alumina and heat-treated at 1250 °C for various periods. Sample dimensions and weight were measured as a function of heat treatment time. The conversion of α-Alumina in the α-Alumina + YSZ composite into Naβ”AY occurred by coupled diffusion of sodium ions through Na-β”-alumina and of oxygen ions through YSZ, effectively diffusing Na2O. From the analysis of the time dependence of sample mass and dimensions, the effective diffusion coefficient of Na2O through the sample, Deff, was estimated to be 1.74 × 10−7 cm2 s−1, and the effective interface transfer parameter, keff, was estimated as 2.33 × 10−6 cm s−1. By depositing a thin alumina coating layer on top of the bulk composite, the chemical diffusion coefficient of oxygen through single phase Na-β”-alumina was estimated as 4.35 × 10−10 cm2 s−1. An AC impedance measurement was performed on a fully converted Naβ”AY composite, and the conductivity of the composite electrolyte was 1.3 × 10−1 S cm−1 at 300 °C and 1.6 × 10−3 S cm−1 at 25 °C, indicating promising applications in solid state or molten salt batteries at low to intermediate temperatures.
Removal of Contaminants from Water by Membrane Filtration: A Review
Authors: Cevallos-Mendoza Jaime , Amorim Célia , Rodríguez-Díaz Joan , Montenegro Maria
Published: May 2022

Drinking water sources are increasingly subject to various types of contamination due to anthropogenic factors and require proper treatment to remove disease-causing agents. Public drinking water systems use different treatment methods to provide safe and quality drinking water to populations. However, they are ineffective in removing contaminants that are considered a danger to the environment and therefore to humans. Several alternative treatment processes have been proposed, such as membrane filtration, as final purification methods. This paper aims to summarize the type of pollutant compounds, filtration processes, and membranes that have been most studied in this area with particular emphasis on how the modification of membranes, either the manufacturing process or the incorporation of nanomaterials, influences their performance.
Stress Response Behavior, Microstructure Evolution and Constitutive Modeling of 22MnB5 Boron Steel under Isothermal Tensile Load
Authors: Zhou Qian , Guo Pengcheng , Qin Feng
Published: May 2022

22MnB5 boron steel has become one of the main choices for lightweight vehicles due to its extremely high mechanical properties. To explore the intrinsic relationship between the thermoforming process and thermo-mechanical behavior for constitutive modeling and thermoforming of vehicle structure, thermal tensile tests in wide ranges of deformation temperature (500 °C to 950 °C) and strain rate (0.01 s−1 to 10 s−1) were performed using a Gleeble-1500D thermal simulator with hot-rolled 22MnB5 boron steel. With increasing applied strain and strain rate, the flow stress increases gradually and then tends to saturation after reaching peak stress, except for that at 0.01 s−1 and 500 °C. With increasing deformation temperature, the microstructure transforms from a mixture of bainite, ferrite and pearlite to lath-shaped martensite accompanied with some residual austenite. At 950 °C, the average size of martensite decreases with increasing applied strain rate. After thermoforming with austenitizing temperature of 950 °C, lath-shaped martensite accompanied with some residual austenite is obtained in a thermoformed U-shaped structural part, resulting in a dramatical increase in tensile strength. In contrast, the tensile strength of sidewall is slightly higher than that of bottom. Based on the Arrhenius-type constitutive model, a modified constitutive model is constructed with a relative error of less than 5%, which can well describe the flow stress behavior of the studied 22MnB5 boron steel.
Microstructure and Mechanical Properties of Low-Density, B2-Ordered AlNbZrTix Multi-Principal Element Alloys
Authors: Tang Qunhua , Su Honghong , Peng Shilong , Chen Wei , Dai Pinqiang
Published: May 2022

Low-density multi-principal element alloys (MPEAs) combining a high specific strength and considerable ductility have remained a research hotspot, due to their promising prospects for energy-saving industrial applications. Light Ti-containing AlNbZrTix (x = 1−3) MPEAs were designed and prepared by induction melting and annealing. As the Ti content increases, the microstructure of these MPEAs evolves from dual phase (B2-ordered and Zr5Al3-type structure) into a single-phase B2-ordered structure, while the density reduces by ~8.7%, from ~5.85 g·cm−3 (x = 1) to ~5.34 g·cm−3 (x = 3). Unexpectedly, the AlNbZrTix (x = 1, 2, 3) alloys possess high specific yield strengths of ~270 kPa·m3·kg−1, ~221 kPa·m3·kg−1, >208 kPa·m3·kg−1, along with excellent fracture strains of ~17.8%, 21.8%, and >50%, respectively. These combined compressive properties are superior to the reported data of most BCC/B2-dominant MPEAs. The deformation mechanism of the B2-ordered structure is explained as a dislocation-based mechanism, accompanied by antiphase domains. Here, the effect of Ti on the microstructure and compressive properties of AlNbZrTix MPEAs was investigated, providing scientific support for the development of advanced low-density materials.
Cross-Scale Simulation Research on the Macro/Microstructure of TC4 Alloy Wire Laser Additive Manufacturing
Authors: Wang Yongbiao , Chen Cong , Liu Xintian , Wang Jiaxin , Zhang Yang , Long Weimin , Guan Shaokang , Peng Liming
Published: May 2022

A cross-scale model of macro-micro coupling is established for the wire laser additive manufacturing process of the TC4 titanium alloy. The model reproduces the dynamic evolution process of the molten pool shape, reveals the temperature change law in the molten pool, and simulates the microstructure and morphology of different regions of the molten pool. Finally, the model is used to quantitatively analyze the effects of process parameters (laser power, scanning speed) on the growth morphology of dendrites during solidification. The research shows that with the increase in laser power and the decrease in scanning speed, the peak temperature of the molten pool increases rapidly, and the size of the molten pool increases gradually. When the laser scanning speed is greater than 5 mm/s, the molten pool length decreases significantly. After solidification, an asymmetrically distributed equiaxed grain structure is formed at the upper part of the molten pool, the bottom of the molten pool is made up of slender columnar crystals, and the columnar-to-equiaxed transition (CET) occurs in the middle of the molten pool. With the decrease in laser power and the increase in scanning speed, the growth rate of dendrites becomes faster, the arm spacing and the overall morphology of dendrites become smaller, and the arrangement of columnar crystals have a tighter microstructure.
Experimental Research on the Thermal-Consolidation Compound Forming of Thermosetting Fiber Metal Laminates Design for Complex Structures with Variable Curvature
Authors: Zhang Quanda , Sun Fuzhen , Ji Rigele , Liu Zizhi , Li Huiyu , Wang Yao
Published: May 2022

The glass fiber-reinforced metal laminates (GLARE) cannot be used to form complex laminate structures in the aerospace industry, because there is substantial variation in the plasticity of the heterogeneous materials. Hence, a compound process for composite materials based on the thermoforming technology for aluminum alloy and fiber-reinforced metal laminates (FMLs)-forming technology was proposed; it contains solution heat treatment, thermoforming, quick cold die quenching, artificial aging integrated process (HFQ), and the thermal consolidation of fiber-reinforced metal laminates, and it is named the HFQ-FMLs forming process. In order to test and judge the effect of the heat treatment on the properties of the materials obtained by the new technology, the pure metal sheet and the three kinds of HFQ-FMLs composite laminates fabricated with the different layup method were assessed with the Vickers hardness test and the Charpy impact test at the same time, and they were labeled #1, #2, #3, #4, respectively. In the Charpy impact test, in order to obtain accurate data, the shape and fixing position of the specimen was optimized so that the gap direction was parallel to the loading direction. After the heat treatment, the properties of the aluminum alloy were improved, the hardness will affect the energy absorption of the laminates, and the relationship between the thickness, hardness and impact properties will be analyzed. The hardness test results are 39.9 HV, 37.5 HV, 37.4 HV, 37.1 HV which indicates the pure metal sheet had the greatest hardness, and the greater the thickness of fiber layer, the lower was the hardness of the HFQ-FMLs composite laminate. The impact resistance of the HFQ-FMLs composite laminates was about two times of the pure metal sheet for the same thickness, and the values are 2.3 J, 4.8 J, 4.8 J, 4.8 J, respectively. In addition, the method of laying the fiber layer had no effect on the impact resistance of the composite laminates. For the novel composite laminates subjected to different cutting processes, the scanning electron microscope (SEM) results for the incision morphology suggest that the water cutting process ensures the structural integrity of the composite laminates after the edges’ and holes’ cutting procedures, and the performance maintains continuity.
A Review of Signal Processing Techniques for Ultrasonic Guided Wave Testing
Authors: Diogo Ana Rita , Moreira Bruno , Gouveia Carlos A. J. , Tavares João Manuel R. S.
Published: May 2022

Ultrasonic guided wave testing (UGWT) is a non-destructive testing (NDT) technique commonly used in structural health monitoring to perform wide-range inspection from a single point, thus reducing the time and effort required for NDT. However, the multi-modal and dispersive nature of guided waves makes the extraction of essential information that leads to defect detection an extremely challenging task. The purpose of this article is to give an overview of signal processing techniques used for filtering signals, isolating modes and identifying and localising defects in UGWT. The techniques are summarised and grouped according to the geometry of the studied structures. Although the reviewed techniques have led to satisfactory results, the identification of defects through signal processing remains challenging with space for improvement, particularly by combining signal processing techniques and integrating machine learning algorithms.
Experimental and Numerical Analysis of Steel Beams’ Efficiency with Different Shapes of Corrugated Webs under Free Vibrations
Authors: Sayed Ahmed Mohamed , Elaraki Yassir G. , Elalaoui Oussama
Published: May 2022

The use of corrugated webs in steel beams has become one of the most practical ways to increase the efficiency of beams in resisting loads, natural frequencies, and their resulting displacements. Such a practice has the benefit of achieving the same efficiency as beams with a flat web with a greater thickness. Consequently, the effect of the types of corrugated webs on the flexure mode shapes and natural frequencies in the steel beams has been investigated by analyzing 23 beams with flat and corrugated webs using the finite element (FE) numerical analysis technique. Hence, two flat web steel beams with and without stiffeners were experimentally tested and compared to FE numerical modeling results. Moreover, three types (trapezoidal, rectangular, triangular) of corrugated webs were tested and studied in addition to other parameters such as thickness, width, length, and angle. The investigation results show that FE numerical analysis is very highly accurate in predicting the natural frequencies and flexure mode shape changes of beams with corrugated webs. Accordingly, a saving of at least 33% in thickness could be realized as compared to the flat type. The triangular corrugated web is more efficient in the 1st mode shape, while rectangular is better for the other modes. The effectiveness of the angle for the triangular type in resisting mode shape and frequencies was clearly noticed, and decreases of 0.60 and 0.78, respectively, were recorded. It is also perceived that the effect of thickness and angle is linear for specified cases.
Influence of Y Nano-Oxide and Its Secondary Phase on Microstructure, Mechanical Properties, and Wear Behavior of the Stainless Steel Coatings Fabricated by Plasma Transfer Arc
Authors: Yue Junyu , Sui Yi , Yang Lifeng , Lu Fei , Chen Weidong , Liu Xiaoyu , Sun Xiaohua
Published: May 2022

Rare-earth is an efficient refiner for surface modification of steel material. This study presents the synergistic influence of Y2O3 nanoparticles (YNPs) and Mn-oxide secondary phase on the microstructure and mechanical properties of 14CrSiMnV coating fabricated by plasma transfer arc cladding process. The results indicated that the residual Y accumulated with Mn, forming a secondary phase oxide particle instead of inclusions or slags during rapid cooling solidification of the coating. Due to enlarged equiaxed grains, declining long-range dendritic grains, and secondary phase strengthening, steel coatings present hybrid-type fracture mechanism, less plastic deformation, and third-body interaction. With an optimal addition of YNPs (0.4 wt.%), the mechanical properties of the steel coating are improved, as indicated by the increase of 92.0% in the tensile strength, increase of 55.6% in the elongation, increase of 11.3% in the microhardness, decrease of 22.2% in wear weight loss, and increase of 28.3% in relative wear resistance.
Simplified Elastoplastic Fatigue Correction Factor Analysis Approach Based on Minimum Conservative Margin
Authors: Shao Xuejiao , Du Juan , Fu Xiaolong , Xiong Furui , Li Hui , Tian Jun , Lu Xifeng , Xie Hai
Published: May 2022

ASME and RCC-M codes specify an elastoplastic fatigue analysis technique: a simplified elastoplastic fatigue analysis method based on linear elastic analysis. In this method, the elastic strain range is multiplied by the elastoplastic correction factor (Ke) to envelope the actual plastic strain range for fatigue evaluation. The ASME or RCC-M provide the Ke parameters of typical materials, such as austenitic stainless steel and low alloy steel. However, how can the parameters of the material not included in the codes be determined? Based on the existing material Z2CND18.12 (nitrogen control) in the codes and taking into account various sensitive factors, the minimum conservative margin of Ke for this material is calculated, and then the parameters of nonstandard materials are determined iteratively based on the conservative margin. The sensitive factors include the different structure model, load types, the loading control mode, temperature value and the material constitutive model. Based this approach, the Ke parameters of TA16 are determined and verified by the transient with drastic change in temperature and pressure. The results of the case show that the simplified elastoplastic fatigue analysis can envelope the results of cyclic plastic fatigue analysis. The minimum margin approach established in this paper can reasonably determine the Ke value of materials beyond the ASME and RCC-M codes.
Thermodynamics Analysis and Pilot Study of Reusing Medium and High Alloy Steel Scrap Using Induction Melting and Electroslag Remelting Process
Authors: Wu Liushun , Liu Kunlong , Mei Haiqing , Bao Guangda , Zhou Yun , Wang Haichuan
Published: May 2022

The annual cumulative quantity of high and medium alloy steel scrap has exceeded 10 million tons. Using the traditional smelting process involving electric arc refining in a smelting furnace for these scraps causes high percentages of alloy losses, which decreases the value of the alloy steel scrap and poses environmental threats. Existing studies have rarely focused on separate smelting of the scrap and oxidation behaviors of the alloying elements. Therefore, this study proposes an induction melting and electroslag remelting scheme to process the scrap. Based on this scheme, the effects of the temperature, oxygen content, and element contents on the recovery percentages of the alloying elements were investigated using pilot experiment and thermodynamic analysis. The experimental results showed that the alloying elements (tungsten, chromium, nickel, molybdenum, and vanadium) exhibited recovery percentages of 97.36%, 94.62%, 97.63%, 95.09%, and 89.49%, respectively; furthermore, the impurity content did not increase during smelting. The thermodynamic analysis indicated that an increase in carbon content improved the oxidation resistance of the alloying elements except for nickel, whereas the increases in the contents of oxygen and alloying elements increase their oxidation. Steam partial pressure and air suction dramatically increase the concentrations of nitrogen, hydrogen and oxygen. This scheme is an alternative for smelting medium and high alloy steel scrap, and the thermodynamic analysis provides a theoretical understanding of the oxidation behaviors of the alloying elements in the steel scrap and the control of impurity.
Use of Low Melting Point Metals and Alloys (Tm < 420 °C) as Phase Change Materials: A Review
Authors: Noohi Zohrehsadat , Nosouhian Simin , Niroumand Behzad , Timelli Giulio
Published: May 2022

Phase Change Materials (PCMs) are materials that release or absorb sufficient latent heat at a constant temperature or a relatively narrow temperature range during their solid/liquid transformation to be used for heating or cooling purposes. Although the use of PCMs has increased significantly in recent years, their major applications are limited to Latent Heat Storage (LHS) applications, especially in solar energy systems and buildings. PCMs can be classified according to their composition, working temperature and application. Metallic PCMs appear to be the best alternative to salts and organic materials due to their high conductivity, high latent heat storage capacity and wide-ranging phase change temperature, i.e., melting temperature and chemical compatibility with their containers. This paper reviews the latest achievements in the field of low-melting point metallic PCMs (LMPM-PCMs), i.e., those with melting temperatures of less than 420 °C, based on Zn, Ga, Bi, In and Sn. Pure LMPM-PCMs, alloy LMPM-PCMs and Miscibility Gap Alloy (MGA) LMPM-PCMs are considered. Criteria for the selection of PCMs and their containers are evaluated. The physical properties and chemical stability of metallic PCMs, as well as their applications, are listed, and new application potentials are presented or suggested. In particular, the novel application of metallic PCMs in casting design is demonstrated and suggested.
The Kynurenine Pathway in Obese Middle-Aged Women with Normoglycemia and Type 2 Diabetes
Authors: Kubacka Justyna , Staniszewska Magdalena , Sadok Ilona , Sypniewska Grazyna , Stefanska Anna
Published: May 2022

We examined the relationships of tryptophan (Trp) and the metabolites of the kynurenine pathway (KP) to the occurrence of type 2 diabetes (T2D) and metabolic risk factors in obese middle-aged women. The study included 128 obese women divided into two subgroups: a normoglycemic group (NG, n = 65) and a T2D group (n = 63). The concentrations of serum tryptophan (Trp), kynurenine (Kyn), 3-hydroxykynurenine (3HKyn), quinolinic acid (QA), and kynurenic acid (Kyna) were analyzed using ultra-high-performance liquid chromatography coupled with electrospray ionization/triple quadrupole mass spectrometry. Blood biochemical parameters and anthropometric parameters were measured. The women with T2D had significantly higher Trp, Kyna, Kyna/QA ratio, and Kyna/3HKyn ratio values than the NG women. Logistic regression analysis showed that the concentrations of Trp and Kyna and the values of the Kyna/3HKyn ratio were most strongly associated with T2D occurrence, even after controlling for confounding factors. The model with Trp level and Kyna/3HKyn ratio accounted for 20% of the variation in the presence of T2D. We also showed a different pattern of correlations between kynurenines and metabolic factors in the NG and T2D women, which was mostly reflected in the stronger relationship between BMI and KP metabolites in the NG obese women. An increase in Trp and Kyna levels with an accompanying increase in Kyna/3HKyn ratio value is associated with the occurrence of T2D in obese middle-aged women.
Rifampicin-Mediated Metabolic Changes in Mycobacterium tuberculosis
Authors: Yelamanchi Soujanya D. , Mishra Archita , Behra Santosh Kumar , Karthikkeyan Gayathree , Keshava Prasad Thottethodi Subrahmanya , Surolia Avadhesha
Published: May 2022

Mycobacterium tuberculosis (Mtb) is considered to be a devastating pathogen worldwide, affecting millions of people globally. Several drugs targeting distinct pathways are utilized for the treatment of tuberculosis. Despite the monumental efforts being directed at the discovery of drugs for Mtb, the pathogen has also developed mechanisms to evade the drug action and host processes. Rifampicin was an early anti-tuberculosis drug, and is still being used as the first line of treatment. This study was carried out in order to characterize the in-depth rifampicin-mediated metabolic changes in Mtb, facilitating a better understanding of the physiological processes based on the metabolic pathways and predicted protein interactors associated with the dysregulated metabolome. Although there are various metabolomic studies that have been carried out on rifampicin mutants, this is the first study that reports a large number of significantly altered metabolites in wild type Mtb upon rifampicin treatment. In this study, a total of 173 metabolites, associated with pyrimidine, purine, arginine, phenylalanine, tyrosine, and tryptophan metabolic pathways, were significantly altered by rifampicin. The predicted host protein interactors of the rifampicin-dysregulated Mtb metabolome were implicated in transcription, inflammation, apoptosis, proteolysis, and DNA replication. Further, tricarboxylic acidcycle metabolites, arginine, and phosphoenolpyruvate were validated by multiple-reaction monitoring. This study provides a comprehensive list of altered metabolites that serves as a basis for understanding the rifampicin-mediated metabolic changes, and associated functional processes, in Mtb, which holds therapeutic potential for the treatment of Mtb.
Impact of Skin Tissue Collection Method on Downstream MALDI-Imaging
Authors: Yadav Manoj , Chaudhary Prem Prashant , D’Souza Brandon N. , Spathies Jacquelyn , Myles Ian A.
Published: May 2022

MALDI imaging is a novel technique with which to study the pathophysiologies of diseases. Advancements in the field of metabolomics and lipidomics have been instrumental in mapping the signaling pathways involved in various diseases, such as cancer and neurodegenerative diseases (Parkinson’s). MALDI imaging is flexible and can handle many sample types. Researchers primarily use either formalin-fixed paraffin-embedded (FFPE) or fresh frozen tissue samples to answer their scientific questions. FFPE samples allow for easy long-term storage, but the requirement for extensive sample processing may limit the ability to provide a clear picture of metabolite distribution in biological tissue. Frozen samples require less handling, but present logistical challenges for collection and storage. A few studies, mostly focused on cancer cell lines, have directly compared the results of MALDI imaging using these two tissue fixation approaches. Herein, we directly compared FFPE and fresh frozen sample preparation for murine skin samples, and performed detailed pathway analysis to understand how differences in processing impact MALDI results from otherwise identical tissues. Our results indicate that FFPE and fresh frozen methods differ significantly in the putative identified metabolite content and distribution. The fixation methods shared only 2037 metabolites in positive mode and only 4079 metabolites in negative ion mode. However, both fixation approaches allowed for downstream fluorescent staining, which may save time and resources for samples that are clinically precious. This work represents a direct comparison of the impacts of the two main tissue processing methods on subsequent MALDI-MSI. While our results are similar to previous work in cancer tissue, they provide novel insights for those using MALDI-MSI in skin.
Fibroblast Growth Factor 23 and Cardiovascular Risk in Diabetes Patients—Cardiologists Be Aware
Authors: Kurpas Anna , Supel Karolina , Wieczorkiewicz Paulina , Bodalska Duleba Joanna , Zielinska Marzenna
Published: May 2022

Numerous clinical studies have indicated that elevated FGF23 (fibroblast growth factor 23) levels may be associated with cardiovascular (CV) mortality, especially in patients with chronic kidney disease. The purpose of this study was to examine the hypothesis that FGF23 may be a potent CV risk factor among patients with long-standing type 2 diabetes mellitus (T2DM). Research was performed utilizing patients with T2DM and regular outpatient follow-up care. Baseline characteristics determined by laboratory tests were recorded. Serum FGF23 levels were detected using a sandwich enzyme-linked immunosorbent assay. All patients underwent echocardiograms and 12-lead electrocardiograms. Data records of 102 patients (males: 57%) with a median age of 69 years (interquartile range (IQR) 66.0–74.0) were analyzed. Baseline characteristics indicated that one-third (33%) of patients suffered from ischemic heart disease (IHD), and the median time elapsed since diagnosis with T2DM was 19 years (IQR 14.0–25.0). The hemoglobin A1c, estimated glomerular filtration rate, and FGF23 values were, respectively, as follows: 6.85% (IQR 6.5–7.7), 80 mL/min/1.73 m2 (IQR 70.0–94.0), and 253.0 pg/mL (IQR 218.0–531.0). The study revealed that FGF23 was elevated in all patients, regardless of IHD status. Thus, the role of FGF23 as a CV risk factor should not be overestimated among patients with T2DM and good glycemic control.
Design and Joint Position Control of Bionic Jumping Leg Driven by Pneumatic Artificial Muscles
Authors: Dai Zhenhao , Rao Jinjun , Xu Zili , Lei Jingtao
Published: May 2022

Using the skeletal structure and muscle distribution of the hind limbs of a jumping kangaroo as inspiration, a bionic jumping leg was designed with pneumatic artificial muscles (PAMs) as actuators. Referring to the position of biarticular muscles in kangaroos, we constructed a bionic joint using biarticular and monoarticular muscle arrangements. At the same time, the problem of the joint rotation angle limitations caused by PAM shrinkage was solved, and the range of motion of the bionic joint was improved. Based on the output force model of the PAM, we established a dynamic model of the bionic leg using the Lagrange method. In view of the coupling problem caused by the arrangement of the biarticular muscle, an extended state observer was used for decoupling. The system was decoupled into two single-input and single-output systems, and angle tracking control was carried out using active disturbance rejection control (ADRC). The simulation and experimental results showed that the ADRC algorithm had a better decoupling effect and shorter adjustment time than PID control. The jumping experiments showed that the bionic leg could jump with a horizontal displacement of 320 mm and a vertical displacement of 150 mm.
Automated Open-Hardware Multiwell Imaging Station for Microorganisms Observation
Authors: Gervasi Alain , Cardol Pierre , Meyer Patrick E.
Published: May 2022

Bright field microscopes are particularly useful tools for biologists for cell and tissue observation, phenotyping, cell counting, and so on. Direct cell observation provides a wealth of information on cells’ nature and physiological condition. Microscopic analyses are, however, time-consuming and usually not easy to parallelize. We describe the fabrication of a stand-alone microscope able to automatically collect samples with 3D printed pumps, and capture images at up to 50× optical magnification with a digital camera at a good throughput (up to 24 different samples can be collected and scanned in less than 10 min). Furthermore, the proposed device can store and analyze pictures using computer vision algorithms running on a low power integrated single board computer. Our device can perform a large set of tasks, with minimal human intervention, that no single commercially available machine can perform. The proposed open-hardware device has a modular design and can be freely reproduced at a very competitive price with the use of widely documented and user-friendly components such as Arduino, Raspberry pi, and 3D printers.
Efficient Cell Impedance Measurement by Dielectrophoretic Cell Accumulation and Evaluation of Chondrogenic Phenotypes
Authors: Nakata Natsumi , Ishibashi Yuko , Miyata Shogo
Published: May 2022

The quantitative and functional analyses of cells are important for cell-based therapies. In this study, to establish the quantitative cell analysis method, we propose an impedance measurement method supported by dielectrophoretic cell accumulation. An impedance measurement and dielectrophoresis device was constructed using opposing comb-shaped electrodes. Using dielectrophoresis, cells were accumulated to form chain-like aggregates on the electrodes to improve the measurement sensitivity of the electrical impedance device. To validate the proposed method, the electrical impedance and capacitance of primary and de-differentiated chondrocytes were measured. As a result, the impedance of the chondrocytes decreased with an increase in the passage number, whereas the capacitance increased. Therefore, the impedance measurement method proposed in this study has the potential to identify chondrocyte phenotypes.