bluuebunny/crossref_metadata_2025 huggingface dataset:
Pitch and Plasticity: Insights from the Pitch Matching of Chords by Musicians with Absolute and Relative Pitch
Authors: McLachlan Neil , Marco David , Wilson Sarah
Published: December 2013

Absolute pitch (AP) is a form of sound recognition in which musical note names are associated with discrete musical pitch categories. The accuracy of pitch matching by non-AP musicians for chords has recently been shown to depend on stimulus familiarity, pointing to a role of spectral recognition mechanisms in the early stages of pitch processing. Here we show that pitch matching accuracy by AP musicians was also dependent on their familiarity with the chord stimulus. This suggests that the pitch matching abilities of both AP and non-AP musicians for concurrently presented pitches are dependent on initial recognition of the chord. The dual mechanism model of pitch perception previously proposed by the authors suggests that spectral processing associated with sound recognition primes waveform processing to extract stimulus periodicity and refine pitch perception. The findings presented in this paper are consistent with the dual mechanism model of pitch, and in the case of AP musicians, the formation of nominal pitch categories based on both spectral and periodicity information.
Research on Climate Change and Its Impacts Needs Freedom of Research
Authors: Mölders Nicole
Published: December 2013

Climate change captured my interest as a teenager when, at the dining table, my dad talked about potential anthropogenic climate changes. He brought up subjects such as “climate could change if the Siberian Rivers were to be deviated to the South for irrigation of the (semi) arid areas of the former Soviet Union”. Other subjects were afforestation in the Sahel to enhance precipitation recycling, deforestation in the Tropics that could have worldwide impacts on climate, the local climate impacts of the Merowe High Dam in its vicinity and downstream, Atlantropa, a new ice age, and the increase in days with sunshine after the introduction of the high-chimney policy in the Rhein-Ruhr area, just to mention a few. [...]
Patterns of Evolutionary Speed: In Search of a Causal Mechanism
Authors: Gillman Len , Wright Shane
Published: December 2013

The “integrated evolutionary speed hypothesis” proposes that the rate of genetic evolution influences all major biogeographical patterns of diversity including those associated with temperature, water availability, productivity, spatial heterogeneity and area. Consistent with this theory, rates of genetic evolution correspond with patterns of diversity and diversification. Here we review the mechanisms that have been proposed to explain these biogeographic patterns in rates of genetic evolution. Tests of several proposed mechanisms have produced equivocal results, whereas others such as those invoking annual metabolic activity, or a “Red Queen” effect, remain unexplored. However, rates of genetic evolution have been associated with both productivity mediated rates of germ cell division and active metabolic rates and these explanations therefore justify further empirical investigation.
Non-Equilibrium Statistical Mechanics Inspired by Modern Information Theory
Authors: Dahlsten Oscar
Published: December 2013

A collection of recent papers revisit how to quantify the relationship between information and work in the light of modern information theory, so-called single-shot information theory. This is an introduction to those papers, from the perspective of the author. Many of the results may be viewed as a quantification of how much work a generalized Maxwell’s daemon can extract as a function of its extra information. These expressions do not in general involve the Shannon/von Neumann entropy but rather quantities from single-shot information theory. In a limit of large systems composed of many identical and independent parts the Shannon/von Neumann entropy is recovered.
How Efficient are Agitators in Biogas Digesters? Determination of the Efficiency of Submersible Motor Mixers and Incline Agitators by Measuring Nutrient Distribution in Full-Scale Agricultural Biogas Digesters
Authors: Lemmer Andreas , Naegele Hans-Joachim , Sondermann Jana
Published: December 2013

The goal of this work was to evaluate the efficiency of two different agitation systems by measuring the nutrient distribution in a digester fed with renewable energy crops and animal manure. The study was carried out at the practical research biogas plant of Hohenheim University. A unique probe sampling system has been developed that allows probe sampling from the top of the concrete roof into different parts and heights of the digester. The samples were then analyzed in the laboratory for natural fatty acids concentrations. Three different agitation setups were chosen for evaluation at continuous stirring and feeding procedures. The results showed that the analysis approach for agitator optimization through direct measurement of the nutrients distribution in the digester is promising. The type of the agitators and the agitation regime showed significant differences on local concentrations of organic acids, which are not correlated to the dry matter content. Simultaneous measurements on electric energy consumption of the different agitator types verify that by using the slow-moving incline agitator with large propeller diameters in favor of the fast-moving submersible mixer with smaller propeller diameters, the savings potential rises up to 70% by maintaining the mixing quality.
A Methodology for Defining Electricity Demand in Energy Simulations Referred to the Italian Context
Authors: Caputo Paola , Gaia Costa , Zanotto Valentina
Published: December 2013

Electricity consumption in Europe is constantly increasing, despite the fact that in recent years, huge efforts in terms of programs and regulations have been made towards energy demand reduction and energy systems improvement. Since the electricity demand affects both the operation of the supply and distribution plants and the thermal loads of buildings, the importance of providing a proper definition of demand profiles is evident. The main aim of the paper is to provide a set of standard electricity profiles that can reasonably be adopted as input in energy simulations related to the built environment, with particular regards to the Italian context. The work presented in this paper originated within a wider long lasting research aimed at developing a platform for buildings’ energy simulations at district level, with particular reference to the Italian conditions. In this context, it was necessary to define hourly profiles regarding both occupancy and electricity use for lighting and appliances related to different building uses and typologies. For this purpose, the main methods and references for defining electricity loads in buildings were evaluated and average hourly profiles were accordingly developed for residential and commercial buildings. Then the related internal gains were determined and compared to the current Italian standards.
AC Power Local Network with Multiple Power Routers
Authors: Takahashi Ryo , Kitamori Yutaro , Hikihara Takashi
Published: December 2013

Controlling power flow and achieving appropriate matching between power sources and loads according to the quality of energy is expected to be one of the approaches to reduce wasted energy consumption. A power router, proposed recently, has the capability of realizing circuit switching in a power distribution network. This study focuses on the feasibility of an AC power routing network system composed of multiple power routers. To evaluate the feasibility, we experimentally confirm the circuit switching operation of the parallel and series configurations of the power routers, so that the network system can be designed by the combination of parallel and series configurations.
Recruiting Hard-to-Reach Subjects for Exercise Interventions: A Multi-Centre and Multi-Stage Approach Targeting General Practitioners and Their Community-Dwelling and Mobility-Limited Patients
Authors: Brach Michael , Moschny Anna , Bücker Bettina , Klaaßen-Mielke Renate , Trampisch Matthias , Wilm Stefan , Platen Petra , Hinrichs Timo
Published: December 2013

The general practitioner (GP)’s practice appears to be an ideal venue for recruiting community-dwelling older adults with limited mobility. This study (Current Controlled Trials ISRCTN17727272) aimed at evaluating the recruiting process used for a multi-centre exercise intervention (HOMEfit). Each of six steps resulted in an absolute number of patients (N1–N6). Sex and age (for N4–N6) and reasons for dropping out were assessed. Patient database screening (N1–N3) at 15 GP practices yielded N1 = 5,990 patients aged 70 and above who had visited their GP within the past 6 months, N2 = 5,467 after exclusion of institutionalised patients, N3 = 1,545 patients eligible. Using a pre-defined limitation algorithm in order to conserve the practices’ resources resulted in N4 = 1,214 patients (80.3 ± 5.6 years, 68% female), who were then officially invited to the final assessment of eligibility at the GP’s practice. N5 = 434 patients (79.5 ± 5.4 years, 69% female) attended the practice screening (n = 13 of whom had not received an official invitation). Finally, N6 = 209 (79.8 ± 5.2 years, 74% female) were randomised after they were judged eligible and had given their written informed consent to participate in the randomised controlled trial (overall recruitment rate: 4.4%). The general strategy of utilising a GP’s practice to recruit the target group proved beneficial. The data and experiences presented here can help planners of future exercise-intervention studies.
Indicators for Healthy Ageing — A Debate
Authors: Fuchs Judith , Scheidt-Nave Christa , Hinrichs Timo , Mergenthaler Andreas , Stein Janine , Riedel-Heller Steffi , Grill Eva
Published: December 2013

Definitions of healthy ageing include survival to a specific age, being free of chronic diseases, autonomy in activities of daily living, wellbeing, good quality of life, high social participation, only mild cognitive or functional impairment, and little or no disability. The working group Epidemiology of Ageing of the German Association of Epidemiology organized a workshop in 2012 with the aim to present different indicators used in German studies and to discuss their impact on health for an ageing middle-European population. Workshop presentations focused on prevalence of chronic diseases and multimorbidity, development of healthy life expectancy at the transition to oldest-age, physical activity, assessment of cognitive capability, and functioning and disability in old age. The communication describes the results regarding specific indicators for Germany, and hereby contributes to the further development of a set of indicators for the assessment of healthy ageing.
Associations of Job Stress Indicators with Oxidative Biomarkers in Japanese Men and Women
Authors: Takaki Jiro
Published: December 2013

Some researchers have suggested that oxidative damage may be one of the mechanisms linking job stress with coronary heart disease. The aim of this study was to investigate the association between job stress indicators and oxidative biomarkers. The study included 567 subjects (272 men, 295 women) who answered questionnaires related to their work and underwent a medical examination. Job stress evaluated using the demands-control-support model was measured using the Job Content Questionnaire. Effort-reward imbalance was measured using the Effort-Reward Imbalance Questionnaire. Urinary hydrogen peroxide (H2O2) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were measured by the modified ferrous ion oxidation xylenol orange version-1 method and enzyme-linked immunosorbent assay, respectively. In men, the changes in the odds ratios for high urinary H2O2 associated with a 1-standard-deviation (SD) increase in worksite social support were 0.69 (95% confidence interval (CI) 0.53, 0.91) univariately and 0.68 (95%CI 0.51, 0.90) after adjustment for covariates. The change in the odds ratio for high urinary H2O2 associated with a 1-SD increase in effort-reward ratio was 1.35 (95% CI 1.03, 1.78) after adjustment for covariates. In women, there were no significant associations of the two job stress indicators with urinary H2O2 and 8-OHdG levels after adjustment for covariates (p > 0.05).
Lead Ions Encapsulated in Liposomes and Their Effect on Staphylococcus aureus
Authors: Kensova Renata , Blazkova Iva , Konecna Marie , Kopel Pavel , Chudobova Dagmar , Zitka Ondrej , Vaculovicova Marketa , Hynek David , Adam Vojtech , Beklova Miroslava , Kizek Rene
Published: December 2013

The aim of the study was the preparation of a liposome complex with encapsulated lead ions, which were electrochemically detected. In particular, experiments were focused on the potential of using an electrochemical method for the determination of free and liposome-encapsulated lead and determination of the encapsulation efficiency preventing the lead toxicity. Primarily, encapsulation of lead ions in liposomes and confirmation of successful encapsulation by electrochemical methods was done. Further, the reduction effect of the liposome matrix on the detected electrochemical signal was monitored. Besides encapsulation itself, comparison of toxicity of free lead ions and lead ions encapsulated in liposome was tested. The calculated IC50 values for evaluating the lead cytotoxicity showed significant differences between the lead enclosed in liposomes (28 µM) and free lead ions (237 µM). From the cytotoxicity studies on the bacterial strain of S. aureus it was observed that the free lead ions are less toxic in comparison with lead encapsulated in liposomes. Liposomes appear to be a suitable carrier of various substances through the inner cavity. Due to the liposome structure the lead enclosed in the liposome is more easily accepted into the cell structure and the toxicity of the enclosed lead is higher in comparison to free lead ions.
A Microfluidic Device for Continuous Sensing of Systemic Acute Toxicants in Drinking Water
Authors: Zhao Xinyan , Dong Tao
Published: December 2013

A bioluminescent-cell-based microfluidic device for sensing toxicants in drinking water was designed and fabricated. The system employed Vibrio fischeri cells as broad-spectrum sensors to monitor potential systemic cell toxicants in water, such as heavy metal ions and phenol. Specifically, the chip was designed for continuous detection. The chip design included two counter-flow micromixers, a T-junction droplet generator and six spiral microchannels. The cell suspension and water sample were introduced into the micromixers and dispersed into droplets in the air flow. This guaranteed sufficient oxygen supply for the cell sensors. Copper (Cu2+), zinc (Zn2+), potassium dichromate and 3,5-dichlorophenol were selected as typical toxicants to validate the sensing system. Preliminary tests verified that the system was an effective screening tool for acute toxicants although it could not recognize or quantify specific toxicants. A distinct non-linear relationship was observed between the zinc ion concentration and the Relative Luminescence Units (RLU) obtained during testing. Thus, the concentration of simple toxic chemicals in water can be roughly estimated by this system. The proposed device shows great promise for an early warning system for water safety.
Drainage Structure Datasets and Effects on LiDAR-Derived Surface Flow Modeling
Authors: Li Ruopu , Tang Zhenghong , Li Xu , Winter Jessie
Published: December 2013

With extraordinary resolution and accuracy, Light Detection and Ranging (LiDAR)-derived digital elevation models (DEMs) have been increasingly used for watershed analyses and modeling by hydrologists, planners and engineers. Such high-accuracy DEMs have demonstrated their effectiveness in delineating watershed and drainage patterns at fine scales in low-relief terrains. However, these high-resolution datasets are usually only available as topographic DEMs rather than hydrologic DEMs, presenting greater land roughness that can affect natural flow accumulation. Specifically, locations of drainage structures such as road culverts and bridges were simulated as barriers to the passage of drainage. This paper proposed a geospatial method for producing LiDAR-derived hydrologic DEMs, which incorporates data collection of drainage structures (i.e., culverts and bridges), data preprocessing and burning of the drainage structures into DEMs. A case study of GIS-based watershed modeling in South Central Nebraska showed improved simulated surface water derivatives after the drainage structures were burned into the LiDAR-derived topographic DEMs. The paper culminates in a proposal and discussion of establishing a national or statewide drainage structure dataset.
Identification and Quantification of the Main Active Anticancer Alkaloids from the Root of Glaucium flavum
Authors: Bournine Lamine , Bensalem Sihem , Wauters Jean-Noël , Iguer-Ouada Mokrane , Maiza-Benabdesselam Fadila , Bedjou Fatiha , Castronovo Vincent , Bellahcène Akeila , Tits Monique , Frédérich Michel
Published: December 2013

Glaucium flavum is used in Algerian folk medicine to remove warts (benign tumors). Its local appellations are Cheqiq el-asfar and Qarn el-djedyane. We have recently reported the anti-tumoral activity of Glaucium flavum root alkaloid extract against human cancer cells, in vitro and in vivo. The principal identified alkaloid in the extract was protopine. This study aims to determine which component(s) of Glaucium flavum root extract might possess potent antitumor activity on human cancer cells. Quantitative estimation of Glaucium flavum alkaloids was realized by HPLC-DAD. Glaucium flavum effect on human normal and cancer cell viability was determined using WST-1 assay. Quantification of alkaloids in Glaucium flavum revealed that the dried root part contained 0.84% of protopine and 0.07% of bocconoline (w/w), while the dried aerial part contained only 0.08% of protopine, glaucine as the main alkaloid, and no bocconoline. In vitro evaluation of the growth inhibitory activity on breast cancer and normal cells demonstrated that purified protopine did not reproduce the full cytotoxic activity of the alkaloid root extract on cancer cell lines. On the other hand, bocconoline inhibited strongly the viability of cancer cells with an IC50 of 7.8 µM and only a low cytotoxic effect was observed against normal human cells. Our results showed for the first time that protopine is the major root alkaloid of Glaucium flavum. Finally, we are the first to demonstrate a specific anticancer effect of Glaucium flavum root extract against breast cancer cells, which can be attributed, at least in part, to bocconoline.
New Mononuclear Cu(II) Complexes and 1D Chains with 4-Amino-4H-1,2,4-triazole
Authors: Dîrtu Marinela , Boland Yves , Gillard Damien , Tinant Bernard , Robeyns Koen , Safin Damir , Devlin Eamonn , Sanakis Yiannis , Garcia Yann
Published: December 2013

The crystal structures of two mononuclear Cu(II) NH2trz complexes [Cu(NH2trz)4(H2O)](AsF6)2 (I) and [Cu(NH2trz)4(H2O)](PF6)2 (II) as well as two coordination polymers [Cu(μ2-NH2trz)2Cl]Cl∙H2O (III) and [Cu(μ2-NH2trz)2Cl] (SiF6)0.5∙1.5H2O (IV) are presented. Cationic 1D chains with bridging bis-monodentate μ2-coordinated NH2trz and bridging μ2-coordinated chloride ligands are present in III and IV. In these coordination polymers, the Cu(II) ions are strongly antiferromagnetically coupled with J = −128.4 cm−1 for III and J = −143 cm−1 for IV (H = −JΣSiSi+1), due to the nature of the bridges between spin centers. Inter-chain interactions present in the crystal structures were taken into consideration, as well as g factors, which were determined experimentally, for the quantitative modeling of their magnetic properties.
Assessing the Sustainability of Different Small-Scale Livestock Production Systems in the Afar Region, Ethiopia
Authors: Atanga Ngufor , Treydte Anna , Birner Regina
Published: December 2013

Livestock production is a key income source in eastern Africa, and 80% of the total agricultural land is used for livestock herding. Hence, ecological and socio-economically sustainable rangeland management is crucial. Our study aimed at selecting operational economic, environmental and social sustainability indicators for three main pastoral (P), agro-pastoral (AP), and landless intensive (LI) small scale livestock production systems for use in sustainability assessment in Ethiopia. Quantitative and qualitative data were collected through grey literature and semi-structured interviews, assessing livestock and feed resources, production technology, land tenure, financial and gender issues. Our results suggested that feed shortages (FS) are directly related to grazing pressure (G) and inversely related to grass recovery rates (R). According to our indicators, AP was the most sustainable while P and LI were only conditionally sustainable production systems. 93% of 82 interviewees claimed that private land ownership was the best land tenure incentive for efficient rangeland management. Farmers perceived Prosopis juliflora expansion, sporadic rainfall, and disease infestation as the most significant causes for decreasing livestock productivity. Landless intensive farmers had the highest equality in income distribution (Gini Index: GI = 0.4), followed by P and AP (each with a GI = 0.5). Neither educational background nor income seemed to determine grazing species conservation efforts. We claimed that sustainability indicators are valuable tools to highlight shortcomings and strengths of the three main livestock production systems and help with future livestock management in Ethiopia. Selecting suitable indicators, however, is crucial as data requirements and availability can vary across livestock systems.
New Sinularianin Sesquiterpenes from Soft Coral Sinularia sp.
Authors: Yang Bin , Liao Shengrong , Lin Xiuping , Wang Junfeng , Liu Juan , Zhou Xuefeng , Yang Xianwen , Liu Yonghong
Published: December 2013

Four new sesquiterpenes, sinularianins C–F (3–6), together with known sinularianins A (1) and B (2) were identified from a South China Sea soft coral Sinularia sp. Compounds 1–6 were evaluated for inhibition of NF-κB activation using the cell-based HEK293 NF-κB luciferase reporter gene assay. Compounds 1 and 4 were exhibited a potent effect with inhibitory rates of 41.3% and 43.0% at the concentration of 10 µg/mL, respectively.
A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata
Authors: Xu Jian , Chen Zhiyan , Song Liyan , Chen Lili , Zhu Jianhua , Lv Shuangshuang , Yu Rongmin
Published: December 2013

A new in vitro anti-tumor polypeptide, coded as J2-C3, was isolated from Arca inflata Reeve and purified by diethyl-aminoethanol (DEAE)-sepharose Fast Flow anion exchange and phenyl sepharose CL-4B hydrophobic chromatography. J2-C3 was identified to be a homogeneous compound by native polyacrylamide gel electrophoresis (Native-PAGE). The purity of J2-C3 was over 99% in reversed phase-high performance liquid chromatography (RP-HPLC). The molecular weight was determined as 20,538.0 Da by electrospray-ionization mass spectrometry (ESI-MS/MS). J2-C3 was rich in Glx (Gln + Glu), Lys, and Asx (Asp + Asn) according to amino acid analysis. Four partial amino acid sequences of this peptide were determined as L/ISMEDVEESR, KNGMHSI/LDVNHDGR, AMKI/LI/LNPKKGI/LVPR and AMGAHKPPKGNEL/IGHR via MALDI-TOF/TOF-MS and de novo sequencing. Secondary structural analysis by CD spectroscopy revealed that J2-C3 had the α-helix (45.2%), β-sheet (2.9%), β-turn (26.0%) and random coil (25.9%). The anti-tumor effect of J2-C3 against human tumor cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the IC50 values of J2-C3 were 65.57, 93.33 and 122.95 µg/mL against A549, HT-29 and HepG2 cell lines, respectively. Therefore, J2-C3 might be developed as a potential anti-tumor agent.
Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry
Authors: Yan Jing , Bauer Wolfgang-Andreas , Fischlechner Martin , Hollfelder Florian , Kaminski Clemens , Huck Wilhelm
Published: December 2013

Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
Authors: Banaeiyan Amin , Ahmadpour Doryaneh , Adiels Caroline , Goksör Mattias
Published: December 2013

The possibility to conduct complete cell assays under a precisely controlled environment while consuming minor amounts of chemicals and precious drugs have made microfluidics an interesting candidate for quantitative single-cell studies. Here, we present an application-specific microfluidic device, cellcomb, capable of conducting high-throughput single-cell experiments. The system employs pure hydrodynamic forces for easy cell trapping and is readily fabricated in polydimethylsiloxane (PDMS) using soft lithography techniques. The cell-trapping array consists of V-shaped pockets designed to accommodate up to six Saccharomyces cerevisiae (yeast cells) with the average diameter of 4 μm. We used this platform to monitor the impact of flow rate modulation on the arsenite (As(III)) uptake in yeast. Redistribution of a green fluorescent protein (GFP)-tagged version of the heat shock protein Hsp104 was followed over time as read out. Results showed a clear reverse correlation between the arsenite uptake and three different adjusted low = 25 nL min−1, moderate = 50 nL min−1, and high = 100 nL min−1 flow rates. We consider the presented device as the first building block of a future integrated application-specific cell-trapping array that can be used to conduct complete single cell experiments on different cell types.
Control of a Multi-Drug-Resistant Acinetobacter baumannii Outbreak after Orthopedics Department Relocation
Authors: Gogou Vasiliki , Meletis Georgios , Tsitouras Dimosthenis
Published: December 2013

Acinetobacter baumannii clinical isolates have the ability to survive in the hospital niche for prolonged time periods and to develop resistance against multiple antimicrobial agents. Therefore, A. baumannii has emerged as an important cause of nosocomial outbreaks worldwide, especially in critical-care environments such as intensive care units. In the present communication, we report a multi-drug-resistant A. baumannii outbreak that occurred in an orthopedics department in Greece after the admission of a patient previously hospitalized in the intensive care unit of a Greek tertiary care hospital. Despite the implementation of infection control measures, 29 patients were infected, significantly raising their hospitalization periods and treatment costs. Interestingly, the outbreak was put under control after the department’s previously programmed relocation.
Has Multiculturalism Really Failed? A Canadian Muslim Perspective
Authors: Nagra Baljit , Peng Ito
Published: December 2013

In recent years, claims that multiculturalism has created segregated communities, encouraged terrorism, and failed to foster shared national identities in western nations have gained popularity. In this paper, we use young Canadian Muslims’ lived experience of multiculturalism to reflect on this debate. Contrary to popular rhetoric, our interviews of 50 young Muslim adults show that many maintain a dual Canadian-Muslim identity by utilizing the ideology of multiculturalism, even though they are increasingly stigmatized for their religion. These findings lead us to problematize the discourse surrounding the ‘failure’ of multiculturalism and to highlight the contradictions within it.
Design and Simulation of Two Robotic Systems for Automatic Artichoke Harvesting
Authors: Longo Domenico , Muscato Giovanni
Published: December 2013

The target of this research project was a feasibility study for the development of a robot for automatic or semi-automatic artichoke harvesting. During this project, different solutions for the mechanical parts of the machine, its control system and the harvesting tools were investigated. Moreover, in cooperation with the department DISPA of University of Catania, different field structures with different kinds of artichoke cultivars were studied and tested. The results of this research could improve artichoke production for preserves industries. As a first step, an investigation on existing machines has been done. From this research, it has been shown that very few machines exist for this purpose. Based also on previous experiences, some proposals for different robotic systems have been done, while the mobile platform itself was developed within another research project. At the current stage, several different configurations of machines and harvesting end-effectors have been designed and simulated using a 3D CAD environment interfaced with Matlab®. Moreover, as support for one of the proposed machines, an artificial vision algorithm has been developed in order to locate the artichokes on the plant, with respect to the robot, using images taken with a standard webcam.
Spatial Quality Assessment of Pan-Sharpened High Resolution Satellite Imagery Based on an Automatically Estimated Edge Based Metric
Authors: Javan Farzaneh , Samadzadegan Farhad , Reinartz Peter
Published: December 2013

Most of the existing pan-sharpening quality assessment methods consider only the spectral quality and there are just few investigations, which concentrate on spatial characteristics. Spatial quality of pan-sharpened images is vital in elaborating the capability of object extraction, identification, or reconstruction, especially regarding man-made objects and their application for large scale mapping in urban areas. This paper presents an Edge based image Fusion Metric (EFM) for spatial quality evaluation of pan-sharpening in high resolution satellite imagery. Considering Modulation Transfer Function (MTF) as a precise measurement of edge response, MTFs of pan-sharpened images are assessed and compared to those obtained from the original multispectral or panchromatic images. Spatial quality assessment of pan-sharpening is done by comparison of MTF curves of the pan-sharpened and reference images. The capability of the proposed method is evaluated by quality assessment of two different residential and industrial urban areas of WorldView-2 pan-sharpened images. Obtained results clearly show the wide spatial discrepancy in quality of Pan-sharpened images, resulting from different fusion methods, and confirm the need for spatial quality assessment of fused products. The results also prove the capability of the proposed EFM as a powerful tool for evaluation and comparison of different image fusion techniques and products.
Orthogonal Equations of Multi-Spectral Satellite Imagery for the Identification of Un-Excavated Archaeological Sites
Authors: Agapiou Athos , Alexakis Dimitrios , Sarris Apostolos , Hadjimitsis Diofantos
Published: December 2013

This paper aims to introduce new linear orthogonal equations for different satellite data derived from QuickBird; IKONOS; WorldView-2; GeoEye-1, ASTER; Landsat 4 TM and Landsat 7 ETM+ sensors, in order to enhance the exposure of crop marks. The latest are of significant value for the detection of buried archaeological features using remote sensing techniques. The proposed transformations, re-projects the initial VNIR bands of the satellite image, into a new 3D coordinate system where the first component is the so called “crop mark”, the second component “vegetation” and the third component “soil”. For the purpose of this study, a large ground spectral signature database has been explored and analyzed separately for each different satellite image. The narrow band reflectance has been re-calculated using the Relative Spectral Response filters of each sensor, and then a PCA analysis was carried out. Subsequently, the first three PCA components were rotated in order to enhance the detection of crop marks. Finally, all proposed transformations have been successfully evaluated in different existing archaeological sites and some interesting crop marks have been exposed.
Temporal and Seasonal Variations of the Hot Spring Basin Hydrothermal System, Yellowstone National Park, USA
Authors: Jaworowski Cheryl , Heasler Henry , Neale Christopher , Saravanan Sivarajan , Masih Ashish
Published: December 2013

Monitoring Yellowstone National Park’s hydrothermal systems and establishing hydrothermal baselines are the main goals of an ongoing collaborative effort between Yellowstone National Park’s Geology program and Utah State University’s Remote Sensing Services Laboratory. During the first years of this research effort, improvements were made in image acquisition, processing and calibration. In 2007, a broad-band, forward looking infrared (FLIR) camera (8–12 microns) provided reliable airborne images for a hydrothermal baseline of the Hot Spring Basin hydrothermal system. From 2008 to 2011, night-time, airborne thermal infrared image acquisitions during September yielded temperature maps that established the temporal variability of the hydrothermal system. A March 2012 airborne image acquisition provided an initial assessment of seasonal variability. The consistent, high-spatial resolution imagery (~1 m) demonstrates that the technique is robust and repeatable for generating corrected (atmosphere and emissivity) and calibrated temperature maps of the Hot Spring Basin hydrothermal system. Atmospheric conditions before and at flight-time determine the usefulness of the thermal infrared imagery for geohydrologic applications, such as hydrothermal monitoring. Although these ground-surface temperature maps are easily understood, quantification of radiative heat from the Hot Spring Basin hydrothermal system is an estimate of the system’s total energy output. Area is a key parameter for calculating the hydrothermal system’s heat output. Preliminary heat calculations suggest a radiative heat output of ~56 MW to 62 MW for the central Hot Spring Basin hydrothermal system. Challenges still remain in removing the latent solar component within the calibrated, atmospherically adjusted, and emissivity corrected night-time imagery.
A Region Tracking-Based Vehicle Detection Algorithm in Nighttime Traffic Scenes
Authors: Wang Jianqiang , Sun Xiaoyan , Guo Junbin
Published: December 2013

The preceding vehicles detection technique in nighttime traffic scenes is an important part of the advanced driver assistance system (ADAS). This paper proposes a region tracking-based vehicle detection algorithm via the image processing technique. First, the brightness of the taillights during nighttime is used as the typical feature, and we use the existing global detection algorithm to detect and pair the taillights. When the vehicle is detected, a time series analysis model is introduced to predict vehicle positions and the possible region (PR) of the vehicle in the next frame. Then, the vehicle is only detected in the PR. This could reduce the detection time and avoid the false pairing between the bright spots in the PR and the bright spots out of the PR. Additionally, we present a thresholds updating method to make the thresholds adaptive. Finally, experimental studies are provided to demonstrate the application and substantiate the superiority of the proposed algorithm. The results show that the proposed algorithm can simultaneously reduce both the false negative detection rate and the false positive detection rate.
Detection of Driver Drowsiness Using Wavelet Analysis of Heart Rate Variability and a Support Vector Machine Classifier
Authors: Li Gang , Chung Wan-Young
Published: December 2013

Driving while fatigued is just as dangerous as drunk driving and may result in car accidents. Heart rate variability (HRV) analysis has been studied recently for the detection of driver drowsiness. However, the detection reliability has been lower than anticipated, because the HRV signals of drivers were always regarded as stationary signals. The wavelet transform method is a method for analyzing non-stationary signals. The aim of this study is to classify alert and drowsy driving events using the wavelet transform of HRV signals over short time periods and to compare the classification performance of this method with the conventional method that uses fast Fourier transform (FFT)-based features. Based on the standard shortest duration for FFT-based short-term HRV evaluation, the wavelet decomposition is performed on 2-min HRV samples, as well as 1-min and 3-min samples for reference purposes. A receiver operation curve (ROC) analysis and a support vector machine (SVM) classifier are used for feature selection and classification, respectively. The ROC analysis results show that the wavelet-based method performs better than the FFT-based method regardless of the duration of the HRV sample that is used. Finally, based on the real-time requirements for driver drowsiness detection, the SVM classifier is trained using eighty FFT and wavelet-based features that are extracted from 1-min HRV signals from four subjects. The averaged leave-one-out (LOO) classification performance using wavelet-based feature is 95% accuracy, 95% sensitivity, and 95% specificity. This is better than the FFT-based results that have 68.8% accuracy, 62.5% sensitivity, and 75% specificity. In addition, the proposed hardware platform is inexpensive and easy-to-use.
On the Importance of Strengthening Moderate Beliefs in Climate Science to Foster Support for Immediate Action
Authors: Wendling Zachary , Attari Shahzeen , Carley Sanya , Krause Rachel , Warren David , Rupp John , Graham John
Published: December 2013

Whereas many studies focus on climate skeptics to explain the lack of support for immediate action on climate change, this research examines the effect of moderate believers in climate science. Using data from a representative survey of 832 Indiana residents, we find that agreement with basic scientific conclusions about climate change is the strongest predictor of support for immediate action, and the strength of that agreement is an important characteristic of this association. Responses indicate widespread acceptance of climate change, moderate levels of risk perception, and limited support for immediate action. Half of the respondents (50%) preferred “more research” over “immediate action” (38%) and “no action” (12%) as a response to climate change. The probability of preferring immediate action is close to zero for those who strongly or somewhat disbelieve in climate change, but as belief in climate change grows from moderate to strong, the probability of preferring immediate action increases substantially; the strongest believers have a predicted probability of preferring immediate action of 71%. These findings suggest that, instead of simply engaging skeptics, increasing public support for immediate action might entail motivating those with moderate beliefs in climate change to hold their views with greater conviction.
Different Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates
Authors: Rocha Letícia , Ozaki Christiane , Horton Denise , Menezes Caroline , Silva Anderson , Fernandes Irene , Magnoli Fabio , Vaz Tania , Guth Beatriz , Piazza Roxane
Published: December 2013

Enterotoxigenic Escherichia coli (ETEC) produce heat-labile (LT) and/or heat-stable enterotoxins (ST). Despite that, the mechanism of action of both toxins are well known, there is great controversy in the literature concerning the in vitro production and release of LT and, for ST, no major concerns have been discussed. Furthermore, the majority of published papers describe the use of only one or a few ETEC isolates to define the production and release of these toxins, which hinders the detection of ETEC by phenotypic approaches. Thus, the present study was undertaken to obtain a better understanding of ST and LT toxin production and release under laboratory conditions. Accordingly, a collection of 90 LT-, ST-, and ST/LT-producing ETEC isolates was used to determine a protocol for toxin production and release aimed at ETEC detection. For this, we used previously raised anti-LT antibodies and the anti-ST monoclonal and polyclonal antibodies described herein. The presence of bile salts and the use of certain antibiotics improved ETEC toxin production/release. Triton X-100, as chemical treatment, proved to be an alternative method for toxin release. Consequently, a common protocol that can increase the production and release of LT and ST toxins could facilitate and enhance the sensitivity of diagnostic tests for ETEC using the raised and described antibodies in the present work.
Tenofovir or Zidovudine in Second-Line Antiretroviral Therapy after Stavudine Failure in Southern Africa
Authors: Wandeler Gilles , Gerber Florian , Rohr Julia , Chi Benjamin H , Orrell Catherine , Chimbetete Cleophas , Prozesky Hans , Boulle Andrew , Hoffmann Christopher J , Gsponer Thomas , Fox Matthew P , Zwahlen Marcel , Egger Matthias ,   Africa IeDEA Southern
Published: December 2013

Background

There is debate over using tenofovir or zidovudine alongside lamivudine in second-line anti-retroviral therapy (ART) following stavudine failure. We analysed outcomes in cohorts from South Africa, Zambia and Zimbabwe

Methods

Patients aged ≥16 years who switched from a first-line regimen including stavudine to a ritonavir-boosted lopinavir-based second-line regimen with lamivudine or emtricitabine and zidovudine or tenofovir in seven ART programmes in southern Africa were included. We estimated the causal effect of receiving tenofovir or zidovudine on mortality and virological failure using Cox proportional hazards marginal structural models. Its parameters were estimated using inverse probability of treatment weights. Baseline characteristics were age, sex, calendar year and country. CD4+ T-cell count, creatinine and haemoglobin levels were included as time-dependent confounders.

Results

A total of 1,256 patients on second-line ART, including 958 on tenofovir, were analysed. Patients on tenofovir were more likely to have switched to second-line ART in recent years, spent more time on first-line ART (33 versus 24 months) and had lower CD4+ T-cell counts (172 versus 341 cells/ml) at initiation of second-line ART. The adjusted hazard ratio comparing tenofovir with zidovudine was 1.00 (95% CI 0.59, 1.68) for virological failure and 1.40 (0.57, 3.41) for death.

Conclusions

We did not find any difference in treatment outcomes between patients on tenofovir or zidovudine; however, the precision of our estimates was limited. There is an urgent need for randomized trials to inform second-line ART strategies in resource-limited settings.
Presence of Anti-Interferon Antibodies is Not Associated with Non-Response to Pegylated Interferon Treatment in Chronic Hepatitis B
Authors: Arends Pauline , van der Eijk Annemiek A , Sonneveld Milan J , Hansen Bettina E , Janssen Harry LA , Haagmans Bart L
Published: December 2013

Background

Several factors have been related to response to pegylated interferon (PEG-IFN) in chronic hepatitis B (CHB). The occurrence of anti-IFN antibodies is associated with non-response to PEG-IFN in chronic hepatitis C. This study investigated the association between anti-IFN antibodies and response to PEG-IFN in CHB.

Methods

Presence of anti-IFN antibodies was assessed at baseline and at 3 and 6 months post-treatment in 323 CHB patients treated with PEG-IFN for 1 year.

Results

At baseline, anti-IFN antibodies were detected in 112 (35%) patients. Prevalence was higher in HBeAg-negative compared to HBeAg-positive CHB (43% versus 31%, respectively; P=0.03). Detection of anti-IFN antibodies was not associated with age, sex or HBV genotype. Presence of anti-IFN antibodies at baseline was associated with previous IFN therapy failure ( P=0.04), which remained after adjustment for HBeAg status (OR 2.0, 95% CI 1.1, 3.7; P=0.03). Presence of anti-IFN antibodies at baseline was not associated with response, nor with HBV DNA or HBsAg decline (all P-values >0.3). Overall, 56 of 211 (27%) patients without anti-IFN at baseline developed anti-IFN antibodies after PEG-IFN treatment. Response rates did not differ between patients who developed anti-IFN antibodies and patients who did not develop anti-IFN antibodies during treatment ( P=0.1).

Conclusions

Anti-IFN antibodies may frequently be detected in CHB patients, and presence is associated with previous IFN therapy. However, presence or development of anti-IFN antibodies after PEG-IFN therapy is not associated with non-response to PEG-IFN treatment in CHB. There appears to be no future role for anti-IFN antibodies in predicting response to PEG-IFN in CHB.
Inhibition of HBV replication in vivo using helper-dependent adenovirus vectors to deliver antiviral RNA interference expression cassettes
Authors: Crowther Carol , Mowa Mohube B , Ely Abdullah , Arbuthnot Patrick B
Published: December 2013

Background

HBV is hyperendemic to southern Africa and parts of Asia, but licensed antivirals have little effect on limiting life-threatening complications of the infection. Although RNA interference (RNAi)-based gene silencing has shown therapeutic potential, difficulties with delivery of anti-HBV RNAi effectors remain an obstacle to their clinical use. To address concerns about the transient nature of transgene expression and toxicity resulting from immunostimulation by recombinant adenovirus vectors (Ads), utility of RNAi-activating anti-HBV helper-dependent (HD) Ads were assessed in this study.

Methods

Following intravenous administration of 5x109 unmodified or pegylated HD Ad infectious particles to HBV transgenic mice, HBV viral loads and serum HBV surface antigen levels were monitored for 12 weeks. Immunostimulation of HD Ads was assessed by measuring inflammatory cytokines, hepatic function and immune response to the co-delivered LacZ reporter gene.

Results

Unmodified and pegylated HD Ads transduced 80–90% of hepatocytes and expressed short hairpin RNAs (shRNAs) were processed to generate intended HBV-targeting guides. Markers of HBV replication were decreased by approximately 95% and silencing was sustained for 8 weeks. Unmodified HD Ads induced release of proinflammatory cytokines and there was evidence of an adaptive immune response to β-galactosidase. However the HD Ad-induced innate immune response was minimal in preparations that were enriched with infectious particles.

Conclusions

HD Ads have potential utility for delivery of therapeutic HBV-silencing sequences and alterations of these vectors to attenuate their immune responses may further improve their efficacy.
Organisational drivers of capabilities for multi-stakeholder dialogue and knowledge integration
Authors: Veldhuizen M. , Blok V. , Dentoni D.
Published: December 2013

Business and policy actors increasingly make use of multi-stakeholder interactions (MSI) as a corporate social responsibility strategy to understand, influence, harmonise and meet stakeholders’ social, environmental and financial expectations and so to create value. While many researchers and practitioners have recently described the role of MSI for sustainable innovation and development, little is known about how organisations can develop a capability to effectively create and maintain a dialogue with stakeholders and learn from them. The paper explores the organisational characteristics driving two key capabilities needed for effective MSI: stakeholder dialogue and knowledge integration. Based on the empirical evidence from four business cases, the research follows an explorative approach building upon stakeholder and organisational learning theories. Findings indicate that the ‘involvement of senior management and employees’, ‘open culture’, ‘vision towards sustainability’ and ‘hierarchical structure’ are key drivers of stakeholder dialogue and knowledge integration capabilities.