bluuebunny/crossref_metadata_2025 huggingface dataset:
Infrared thermography as a non-invasive method for detecting fear-related responses of cattle to handling procedures
Authors: Stewart M , Schaefer AL , Haley DB , Colyn J , Cook NJ , Stafford KJ , Webster JR
Published: January 2023

Two experiments were conducted to determine whether maximum eye temperature, measured using infrared thermography (IRT), could be a non-invasive technique for detecting responses of cattle to handling procedures. Experiment one used six crossbred heifers randomly assigned to two groups in a crossover design and subjected to i) being hit with a plastic tube on the rump and ii) being startled by the sudden waving of a plastic bag. Experiment two used 32 crossbred bulls randomly assigned to three treatments: i) control, restraint only; ii) electric prod, two brief applications of an electric prod or, iii) startled, as in experiment one, accompanied by shouting. Exit speed (m s−1) was recorded on release from the restraint. Maximum eye temperature was recorded continuously pre- and post-treatment. In experiment one, eye temperature dropped rapidly between 20 and 40 s following both treatments and returned to baseline between 60 and 80 s following hitting and between 100 and 120 s following startling. In experiment two, eye temperature dropped between 0 and 20 s, following both treatments, and returned to baseline by 180 s, following startling plus shouting, but did not return to baseline for five minutes following electric prod. Exit speed tended to be faster following the electric prod. In conclusion, IRT detected responses that were due possibly to fear and/or pain associated with the procedures and may therefore be a useful, non-invasive method for assessing aversiveness of handling practices to cattle.
What causes crowding? Effects of space, facilities and group size on behaviour, with particular reference to furnished cages for hens
Authors: Appleby MC
Published: January 2023

Theoretical models are presented of the effects of space, facilities and group size on the behaviour of chickens at high stocking densities, with relevance for all animals. The appropriateness of each model is supported by published data, although such data are scant for some important variables. Freedom of movement is analysed by taking the area of a hen as 475 cm2 and finding the number of free bird spaces left at different space allowances. This provides support for current recommendations of a maximum of seven laying hens per m2 on deep litter, but suggests that a maximum for broilers of 34 kg/m2 unacceptably restricts freedom of movement. In cages, freedom of movement increases with space allowance per hen, and, for a given space allowance, with cage and group size. Nesting behaviour is analysed for synchrony, which decreases with group size. Perching and feeding are often synchronous and the space needed for these is determined by body width. Recommendations are derived for hens in furnished cages. The main part of the cage should be as large as possible; an absolute minimum of 600 cm2 per bird is suggested, but 675 cm2 per bird is probably the minimum practical. Perch and feeder space should be provided at 14 cm or more per bird, with a possible derogation for light hybrids to 12 cm. The number of nest spaces needed varies with the number of birds, with nest spaces being 300 cm2 each. These recommendations sum to a minimum of 800 cm2 per bird for groups of eight or more, 850 cm2 for groups of four to seven, and 900 cm2 for groups of three or fewer, plus litter area. Crowding is primarily caused by limited space allowance, but for a given space allowance it is worse in small enclosures and groups.
Habituation, capture and relocation of Sykes monkeys (Cercopithecus mitis albotorquatus) on the coast of Kenya
Authors: Moinde NN , Suleman MA , Higashi H , Hau J
Published: January 2023

The objective of this project was to collect scientific data to assist in the development of guidelines for the humane relocation of threatened and endangered arboreal non-human primate species. A troop of 31 Lowland Sykes monkeys (Cercopithecus mitis albotorquatus) was habituated to fruit bait for capture in a village and relocation to a previously selected suitable site in a protected forest reserve approximately 30 km away. Sixty-five percent (n = 20) of the animals were captured and relocated. This subgroup comprised one adult male, eight adult females, two subadult females, three juvenile males, four juvenile females and two infant males. Although the relocated group originated from one single group, post-translocation telemetry signals demonstrated that it split into two groups, which established themselves approximately 2–4 km apart in their new territory; the adult male eventually became solitary. The factors of importance for the successful capture and relocation of forest primates were found to include: proper understanding of troop home-range utilisation and of social bond organisation within the troop, method and period of habituation, method of release, suitability of the new habitat with respect to the ecological niche requirements of the species in question, and the period of post-relocation monitoring.
The ability of laying hens to negotiate perches of different materials with clean or dirty surfaces
Authors: Scott GB , MacAngus G
Published: January 2023

Increasingly, perches for laying hens are being made from metals and plastics. There is nothing in the literature regarding how easily birds jump between perches of different materials, or how their ability to do so changes with faecal contamination of the perches. Forty-four medium hybrid brown hens negotiated perches of wood (5 cm × 5 cm, rounded edges), metal (half-round section, diameter 4 cm) or poly-vinyl-chloride (PVC: circular section, diameter 4 cm), which were either clean or dirty (poultry manure 0.5–1.0 cm deep). The time to jump to the destination perch (0.75 m from the start perch), number of squats (pre-jumping behaviour), slips, failures to jump (in 300 s) and crashes were recorded. Compared to wood and metal perches, birds took significantly longer to jump from PVC perches when they were clean, but there was no difference when the perches were dirty. Birds slipped significantly more on clean metal or PVC perches compared to clean wood perches. The birds took significantly longer to jump from metal or wooden perches when they were dirty compared to when they were clean. These data may suggest that PVC is not a suitable material for perches. Slipperiness is important. The birds apparently found the metal and PVC perch more slippery than the wooden perch, although the metal perch did not cause the birds to delay jumping. A slippery perch may deter the birds from attempting to jump. Manure on the perches reduced the slipperiness of the metal and PVC perches. Once perches become dirty, any welfare issues concerning the risk of injury from slippery perches cease to be as important as the potential slipperiness of the manure itself.
The effect of blindfolding horses on heart rate and behaviour during handling and loading onto transport vehicles
Authors: Parker R , Watson R , Wells E , Brown SN , Nicol CJ , Knowles TG
Published: January 2023

Blindfolding is routinely used to aid the handling and loading of horses that are difficult to control. Fifteen relatively well-behaved horses of varying ages and disciplines were used to investigate the effects of blinkering and blindfolding on behaviour and heart rate in three situations: whilst stabled, when being led in a ménage, and during loading onto a lorry. Heart rate increased in all three situations when a blindfold was used, and when animals were handled by the least experienced of three handlers. The effects of blinkering on heart rate and behaviour were small compared with blindfolding. Overall, blindfolding appeared to make the horses more nervous and difficult to handle. However, the study does not discount the practical application that blindfolding may have for improving welfare and safety when handling certain individual horses. This work forms the basis for further studies involving animals less accustomed or disposed to being handled.
The effect of transport on cortisol, glucose, heart rate, leukocytes and body weight in captive-reared guanacos (Lama guanicoe)
Authors: Zapata B , Gimpel J , Bonacic C , González BA , Riveros JL , Ramírez AM , Bas F , Macdonald DW
Published: January 2023

Current procedures for ranching and sustainable use of guanacos necessitate their transport. Transportation is a risky process for animals, and is a particular concern for wild-caught or semi-domesticated species such as the guanaco — a wild South American camelid species increasingly being established on farms in Chile and Argentina. This study investigated the effect of transport on the physiological and behavioural responses of eight castrated adult male guanacos, transported on a single 2 h journey at a stocking density of 113.5 kg m-2(0.76 m2per animal). Plasma cortisol and blood glucose concentration, total and differential white blood cell (WBC) counts, heart rate, and body weight were measured one week before, immediately before, immediately after, 2 h after and one week after transport. Behavioural responses were recorded during handling prior to loading. Immediately after transport we found significant increases in plasma cortisol concentrations and neutrophil:lymphocyte (N:L) ratio, the latter peaking 2 h after transport. Heart rate increased significantly only during loading, while body weight remained constant throughout. Behavioural responses related to handling (jumping, vocalising, kicking, spitting and urinating) were not associated with the physiological response. All variables returned to pre-transport values within one week. Transport of guanacos under these conditions produced physiological changes similar to those associated with a mild and transient stress response in other species and which, we judge, fall comfortably within acceptable limits for their welfare.
Comparison of the severity of esophagogastric, lung and limb lesions at slaughter in pigs reared under standard and enriched conditions
Authors: Ramis G , Gómez S , Pallarés FJ , Muñoz A
Published: January 2023

Two hundred and ten pigs were reared in three groups (according to genotype) under enriched conditions of large open-front sawdust-bedded barns. Eight hundred and twenty pigs were reared under standard conditions of small 15-animal pens in a conventional barn with partially slatted floors and natural ventilation. Production parameters including percentage mortality, feed conversion rate and average daily weight gain were calculated at the end of the fattening period. Stomachs, limbs and lungs were examined at slaughter in order to compare the number and severity of lesions between the enriched environment groups and the standard environment group. There was a significantly greater number of esophagogastric lesions in the standard environment group than in the enriched environment groups. No esophagogastric ulcers were observed in any pigs from the enriched groups, while 17.5% of stomachs from pigs in the standard environment group displayed this condition. There were no significant differences in the number of lung lesions associated with enzootic pneumonia between pigs from the standard and enriched environment groups. The limbs of animals reared in the standard environment had more lesions, especially in floor-contact areas, than those reared in the enriched environment (23.84% versus 1.08%). The production parameters measured were improved in the pigs from the enriched environment. These results suggest that the welfare of fattening pigs may be improved by the provision of enrichment in their housing environment. Evaluation of esophagogastric ulceration could be a useful indicator of welfare in pigs.
Effect of supplementary ultraviolet lighting on the behaviour and corticosterone levels of Japanese quail chicks
Authors: Smith EL , Greenwood VJ , Goldsmith AR , Cuthill IC
Published: January 2023

Most birds have visual sensitivity to ultraviolet (UV) wavelengths, and this sensitivity appears to play a role in their colour vision. Artificial lighting is normally deficient in UV wavelengths. Hence, there may be welfare implications for captive birds kept under such lighting. We investigated whether the absence of UV wavelengths during rearing adversely affects Japanese quail (Coturnix coturnix japonica). We also investigated the short-term effect of switching from UV-containing to UV-deficient lighting, and vice versa. Stress was assessed by monitoring behaviour and plasma corticosterone levels. We did not detect any significant difference in these variables between birds reared either with or without UV. We conclude that rearing quail in an absence of UV does not appear to have a significant impact on their welfare, as measured using these indicators.
Bowhunting deer
Authors: Gregory NG
Published: January 2023

There are conflicting views about the humaneness of the handbow as a recreational hunting method for deer. Some claim that it is the most humane hunting method, whilst others report higher wounding rates and crippling losses than with the rifle. This commentary summarises the factors affecting the likelihood of a quick death, the types of equipment commonly used, the vital target areas, the influence of blood loss on blood pressure and brain function and the prevalence of wounding during routine bowhunting. Some requirements in Bowhunters Association Codes of Conduct are also described. It is concluded that where bowhunting is allowed, Codes of Conduct should emphasise the hunters' responsibility to track and despatch injured animals, and adherence to the Codes should be encouraged, if not enforced.
The influence of visual stimulation on the behaviour of dogs housed in a rescue shelter
Authors: Graham L , Wells DL , Hepper PG
Published: January 2023

This study explored the influence of five types of visual stimulation on the behaviour of 50 dogs housed in a rescue shelter. These conditions were: one control condition (no visual stimulation) and four experimental conditions (blank television screen, and moving televised images of conspecifics, interspecifics [ie unfamiliar animal species] and humans). The dogs were exposed to each condition for 4 h per day for five days, with an intervening period of two days between conditions. The dogs' behaviour was recorded on days 1, 3 and 5 during each condition. Dogs spent relatively little of the total observation time looking at the television monitors (10.8%). They spent significantly more of their time looking at the moving images of conspecifics, interspecifics and humans than at the blank screen, although their interest in all experimental conditions declined over time. Dogs spent more time at the front of their enclosures during all of the experimental conditions than during the control condition. Images of conspecifics were more likely to attract the dogs to the front of their kennels than the blank screen. The conspecific and human conditions of visual stimulation attracted slightly more attention from the dogs than the interspecific condition, although not significantly. All of the experimental conditions encouraged significantly less vocalisation and movement than the control condition. Overall, the findings suggest that the behaviour of kennelled dogs is influenced by visual stimulation in the form of television programmes. Such animals, however, may not benefit from this type of enrichment to the same degree as species with more well-developed visual systems. The addition of other types of enrichment strategy for dogs housed in rescue shelters is advocated.
Nest-building behaviour in male rats from three inbred strains: BN/HsdCpb, BDIX/Or1Ico and LEW/Mol
Authors: Jegstrup IM , Vestergaard R , Vach W , Ritskes-Hoitinga M
Published: January 2023

Three inbred strains of male laboratory rat (BN/HsdCpb, BDIX/Or1Ico and LEW/Mol) were provided with nest boxes and nest materials, and were observed for nest-building activity. After 7 days, each cage and nest box was examined. Each nest was weighed and scored for complexity, and returned to the cage. This was repeated after a further 7 days and the nest removed completely. This routine was repeated three times. All three strains of rat built nests inside the nest box and showed the same stages of construction. There was a significant increase in nest complexity between day 7 and day 14 in the strains BN and BDIX. Furthermore, BDIX rats used significantly more material for the nests, compared with the BN and LEW rats. In a second experiment, using the same rats, nest material was offered in four different ways. LEW rats used nesting material irrespective of where it was placed; BN rats only used straw placed on the top of the cage when no alternative was provided in the cage, and would not use the nest box roof when it was covered with bedding; whereas BDIX rats would only use nest material placed within the cage and would not use straw placed on the cage lid. BN rats also used nest material to cover the entrance to the nest box, a practice not carried out by the other strains. This study demonstrates that these rat strains have retained their natural nest-building behaviour. We suggest that the correct stimuli must be provided in order for this behaviour to be exhibited; in addition, the way in which the nest materials are provided must be in accordance with strain-specific characteristics.
The use of conjoint analysis to determine the importance of factors that affect on-farm welfare of the dairy cow
Authors: Angus LJ , Bowen H , Gill LAS , Knowles TG , Butterworth A
Published: January 2023

A large range of variables can affect the welfare of the dairy cow, making it difficult to assess the overall ‘level of welfare’ of the individual animal. Two groups of individuals completed a questionnaire based upon the ‘five freedoms’: 26 respondents had expertise either in the field of dairy cow welfare or as practicing veterinary surgeons, and 30 were veterinary students in their penultimate year of study. Conjoint analysis was used to calculate the average importance scores (AIS) for 34 variables presented to the respondents as 52 ‘model cows’ in the form of grouped questions, phrases and pictures. Conjoint analysis identified the most important factors for each ‘freedom’: access to forage, body condition score, foot conformation, hock lesions, and the encouragement required for a dairy cow to walk into the parlour. There was a significant difference between the expert and student groups for seven out of 34 factors, which may be attributed to individual variation of opinion, knowledge, experience and expectation. The factors were ranked within each ‘freedom’ using the experts' AIS but it was not assumed that each freedom had equal ‘weight’; therefore, the factors within each freedom were compared only with factors within the same freedom. These scores produced a weighting scale, which was applied on-farm, in a preliminary exercise comparing ‘model’ and ‘perceived’ welfare scores.
Meta-analysis — a systematic and quantitative review of animal experiments to maximise the information derived
Authors: Phillips CJC
Published: January 2023

Meta-analysis provides a tool to statistically aggregate data from existing randomised controlled animal experiments. The results can then be summarised across a range of conditions and an increased pool of experimental data can be subjected to statistical analysis. New information can be derived, but most frequently the results are a refinement of existing knowledge. By designing experiments and reporting protocols, so that they have the capability of being useful to meta-analyses, maximum benefit can be derived from individual randomised controlled experiments, which may individually have little statistical power, and new avenues for productive research identified. The methodology for meta-analysis is derived from clinical trials in the medical sciences. Now that there is substantial output from animal science experiments, there is an opportunity to apply the technique to these and reduce the need for further experimentation. This paper describes the contribution of meta-analysis to the reduction of animals in research and provides details on data collection, analysis, the models used, and on interpreting and reporting the results. Three applications of meta-analysis to the field of animal science are also briefly described. First, the impact of undernutrition on the production and composition of milk from dairy cows confirmed existing knowledge about partitioning scarce nutrients to milk yield and live weight. Second, increased absorption of cadmium — a widespread toxic element — from organic sources was detected in sheep, which was previously untested. Third, no significant relationships were found between common indicators of undernutrition and weight, and condition score in cattle suggesting that the common indicators used are not suitable as evidence of long term undernutrition. This paper concludes that opportunities exist to increase the information gained from animal experiments by subjecting the results to meta-analysis, particularly if this can be anticipated in advance of study protocols being constructed.
The use of databases, information centres and guidelines when planning research that may involve animals
Authors: Smith AJ , Allen T
Published: January 2023

In many countries scientists planning research that may involve the use of animals are required by law to examine the possibilities for replacement, reduction or refinement (the Three Rs) of these experiments. In addition to the large number of literature databases, there are now many specialist databases specifically addressing the Three Rs. Information centres, with a mandate to assist scientists and lay people locate information on the Three Rs, have also been established. Email discussion lists and their archives constitute another, although less quality-controlled, source of information. Furthermore, guidelines for the care and use of animals in research have been produced both by regulatory bodies and scientific organisations. The growth of the internet has put an enormous amount of data into the public domain, and the problems of accessing relevant information are discussed. Suggestions are also given for search strategies when using these information sources.
The effectiveness of environmental enrichment on reducing stereotypic behaviour in two captive vicugna (Vicugna vicugna)
Authors: Parker M , Goodwin D , Redhead E , Mitchell H
Published: January 2023

Environmental enrichment by increasing foraging behaviour and providing food item choice are widely practised and generally accepted as effective methods for reducing stereotypic behaviour in captive animals. In this study, the effectiveness of increasing foraging patch choice and food item choice on reducing motor stereotypy in two captive vicugna were examined. For the purposes of the study, first, browse was added to the vicugna's enclosure as an additional forage item and, second, the vicugna's normal feed was divided: half being provided in the indoor quarters and half in the outdoor yard. The results revealed that providing browse as an additional forage item increased the observed stereotypic behaviour; however, dividing the vicugna's feed, and therefore increasing forage patch choice, decreased stereotypy. This study was limited because of the small sample size and because the area in which the vicugna were performing stereotypic behaviour was partially visually obscured. However, this study has implications for animal welfare because it highlights the need to evaluate the suitability of foraging enrichment items, and suggests that more research into accommodating the adaptive foraging behaviour of this species in captivity may be necessary.
Animal welfare assurance programs in food production: a framework for assessing the options
Authors: Fraser D
Published: January 2023

Various animal welfare assurance programs are being used to encourage or require the adoption of animal welfare standards in food production, and to assure the public that such standards are followed. The programs involve five main formats. Non-mandatory codes/guidelines are relatively easy to institute and appear well-supported by the industry, but provide only minimal assurance to the public unless measures are taken to ensure compliance. Programs based on government regulations and inter-governmental agreements are more challenging to institute; they are likely to generate less industry acceptance, but may provide more public confidence if enforcement is adequate. Product differentiation programs, and retailer policies requiring products to meet certain standards, serve a range of functions; these may generate public confidence but only for products covered. The various programs include several types of requirements. Requirements that are designed to maintain animal health and functioning have a widely accepted scientific basis, are often easy to incorporate into existing production systems, and often provide economic benefits, but do not fully address public concerns over animal welfare in some cultures. Requirements that address pain, distress and other affective states, and those that accommodate certain natural behaviour, have a growing but less traditional scientific rationale and appear likely to generate public confidence; however, they sometimes require significant changes to existing practices. Requirements for more natural surroundings (outdoor, free-range) seem to generate public confidence, but appear most likely to increase costs, least likely to be supported by the existing industry, and may involve trade-offs with productivity and with other aspects of animal welfare. The various formats and requirements provide a range of policy options for addressing animal welfare concerns in different cultural, industry and market contexts.
A non-intrusive method of assessing plumage condition in commercial flocks of laying hens
Authors: Bright A , Jones TA , Dawkins MS
Published: January 2023

Standard techniques for assessing plumage damage to hens from feather pecking typically require capture and handling. Handling of individual birds for plumage assessment is relatively easy in experimental studies; however, close inspection of individual birds in commercial flocks is less feasible because catching birds is difficult, may compromise bird welfare and affect egg production. The aim of this study was to assess a non-intrusive method for scoring plumage damage in a commercial free-ranging flock of laying hens. Plumage damage was scored within a 2 m distance of the birds, without capture or handling, using a 5-point scale for 5 body regions. The feather scores, recorded at a distance, by two independent scorers were compared (distance scores), and were then compared with feather scores recorded by a scorer who caught and handled the birds to examine the plumage damage closely (capture scores). There was a significant and positive correlation between the distance scores and the capture scores, and the mean correlation coefficient for all plumage score traits was 0.89. There was also a significant and positive correlation between scorers, and the mean correlation coefficient for all plumage score traits was 0.84. The standard deviation of the residual mean difference between scorers and between methods was less than 1 point for individual body regions and less than 1.5 points for the total body score. Large variation in feather damage within a flock and small sample size increased the standard error of the mean total feather score. When feather damage variation within flocks is low (ie little observed feather damage), the current industry standard of scoring a sample of 100 birds is likely to provide a reliable estimate of flock feather damage; however, when there is large variation within birds of a flock (ie considerable observed feather damage) ≥200 birds should be inspected to accurately monitor changes in plumage condition. The non-intrusive method of feather scoring described in this paper may be useful for commercial-scale feather pecking studies or for farmers who need to assess the plumage damage of their flocks reliably, quickly and with minimal disturbance or stress to the birds.
Refinement of the use of non-human primates in scientific research. Part III: refinement of procedures
Authors: Rennie AE , Buchanan-Smith HM
Published: January 2023

There is an ethical and scientific need to minimise the harm experienced by animals used in scientific procedures and to maximise their well-being. Welfare can be improved by the refinement of practice, particularly if these refinements are applied to every aspect of the life of an animal used in the laboratory, from birth to death. Primates are considered likely to have a greater capacity for suffering than other sentient species and therefore refinement of their use is particularly important. The refinement of the human impact on laboratory-housed primates and of housing and husbandry practices are dealt with in parts I and II of this three-part review. In part III, methods of refinement that can be applied specifically to the use of primates in procedures, are summarised and discussed, together with a description of some current practices, and the scientific evidence that suggests that they should no longer be used. Methods of refinement of identification, capture and restraint, sampling, administration of substances, humane endpoints, and euthanasia are included. If these methods are used, taking into account species-specific differences and needs, it is concluded that harm can be minimised and primate welfare improved.
Training laboratory-housed non-human primates, part 1: a UK survey
Authors: Prescott MJ , Buchanan-Smith HM
Published: January 2023

Training using positive reinforcement is increasingly recognised as a valuable tool for the humane and effective management and use of laboratory-housed non-human primates. We utilised a mixed-mode questionnaire to survey use of training and other learning processes (socialisation, habituation and desensitisation) in over half of UK establishments using and breeding primates. The survey demonstrated that there is widespread awareness of training as a refinement technique and appreciation of its diverse benefits, but training is not used as widely or as fully as it might be. This is due to real constraints (principally staff and time and a lack of confidence in ability to train), and perceived constraints (such as a supposed lack of published information on how to train and assessment of the benefits, and an overestimation of the time investment needed). There is also considerable variation between establishments in the purposes of training and techniques used, with a reliance on negative reinforcement in some. We conclude that there is opportunity for refinement of common scientific, veterinary and husbandry procedures (such as blood and urine collection, injection, capture from the group and weighing) through use of positive reinforcement training, especially when combined with appropriate socialisation, habituation and desensitisation. We end this paper with recommendations on best practice, training techniques and staff education.
Physiological response of rabbits to heat, cold, noise and mixing in the context of transport
Authors: De la Fuente J , Díaz MT , Ibáñez M , de Chavarri E González
Published: January 2023

The effects on rabbits of four potential transport-related stressors (heat [HS], cold [CS], noise [NS] and mixing with unfamiliar animals [MS]) on certain physiological and meat quality parameters were studied. These are factors which may act to reduce the welfare of rabbits during their transport to the slaughterhouse. The rabbits were exposed to each potential stressor for four and a half hours prior to slaughter. HS groups showed the highest plasma concentrations of cortisol, lactate and glucose and greater packed cell volume (PCV) and osmolarity than the control group, and the meat exhibited a low initial pH as a direct consequence of lactic acid accumulation. The rabbits exposed to cold (CS) and noise (NS) showed physiological responses to the potential stressor, although to a lesser degree than rabbits exposed to heat. Cold stressed rabbits showed increased levels of creatine kinase (CK) and a higher PCV as well as decreased muscle glycogen concentration compared to the control. Rabbits exposed to noise showed muscular damage as demonstrated by increased levels of CK and lactate dehydrogenase (LDH) activity in the blood and a high final pH in meat. Mixing unfamiliar rabbits (MS) lead to higher CK activity, lower lactate and glucose concentration and the meat pH was slightly higher than the control group. In conclusion, these results suggested that rabbits exposed to heat were the most affected out of all three groups, although cold, noise and mixing with unfamiliar rabbits also had a detrimental effect on physiological and meat quality parameters.
Effect of beak length on feed intake in pigeons (Columba livia f. domestica)
Authors: Savas T , Konyali C , Das G , Yurtman IY
Published: January 2023

This study was undertaken to investigate the effect of beak length on feed intake with regard to animal welfare. The study involved two treatments groups; short beak pigeons (G-SB; n = 7) and normal beak pigeons (G-NB; n = 7) and was carried out in two consecutive trials. Daily feed consumption, meal length and behavioural traits such as aggressive pecking, preening, resting and locomotor activity were recorded under different feeding conditions. In Trial I the birds had free access to food material during a single feeding period each day, whereas in Trial II feeding was terminated when a pigeon from any of the groups that stopped eating first, moved towards water. It took a longer time for the pigeons in the G-SB group to consume the same amount of feed. Furthermore, the pigeons in G-SB were affected significantly in Trial II and lost bodyweight more compared to G-NB. Although no significant difference between the groups on frequency of aggressive pecking during feeding was found in Trial I, restricted feeding significantly increased the frequency of aggressive pecking in the G-SB group in Trial II. The results of the present study suggest that short beak, which is a side-effect of artificial selection for aesthetic purposes, can cause serious welfare problems under restricted environmental conditions.
Sickness behaviour and its relevance to animal welfare assessment at the group level
Authors: Millman ST
Published: January 2023

The inflammatory response evokes changes in behaviour including increased thermoregulatory activities and sleep, reduced social exploration and appetite, and altered food preferences. This sickness response also includes feelings of lethargy, depression, and pain, collectively referred to as ‘malaise’. Recent experiments involving laboratory rodents reveal information about proximate mechanisms of sickness behaviour, but scant information exists about how sickness behaviour is expressed by farmed species or within social environments. The behavioural needs of ill individuals differ from those of conspecifics, and failure to accommodate the needs of ill individuals may exacerbate suffering. Policy makers, industry and animal welfare certification programs recommend hospital pens to address the housing and handling needs of ill livestock and to reduce risks of disease transmission. However, a survey of swine farms in Ontario, Canada revealed deficiencies in the use of hospital pens and gaps in knowledge about best management practices for this vulnerable population. There is considerable scope to improve the welfare and husbandry of ill and at risk animals through effective use of hospital pens and supportive therapies.
Assessing the animals' relationship to humans in tied dairy cows: between-experimenter repeatability of measuring avoidance reactions
Authors: Waiblinger S , Mülleder C , Schmied C , Dembele I
Published: January 2023

Tests for assessing the animal-human relationship in tied dairy cows have been used in experimental research, but the reliability and feasibility of the measures in the on-farm context has not been studied yet. Therefore we investigated the between-experimenter repeatability of avoidance reactions in dairy cows housed in tie-stall systems. Nine farms in Austria with 15-60 tethered dairy cows were visited. Two to three out of four experimenters were testing the avoidance reaction of the individual cows in a balanced order. Cows were approached slowly from the front and the reactions recorded on an 11-point score. The avoidance reaction of each cow was assessed twice by each experimenter and averaged. Also, a farm value per experimenter was calculated (median). Repeatability between experimenters was assessed within farms and at farm level by calculating Spearman rank correlation coefficients. The average between-experimenter correlations within farms ranged from 0.37 to 0.88. At farm level, median of scores ranged from 2.5 to 6.75 and correlated moderately to highly between experimenters from 0.65 to 0.80. Testing the animals on all farms was relatively simple.

The between-experimenter repeatability within farms differed substantially and reasons for this merit further investigation. At farm level repeatability was moderate to sufficiently high. Due to the high feasibility, this measure seems to be promising and should be developed further.
Potential animal-centred indicators of dairy goat welfare
Authors: Mazurek M , Marie M , Desor D
Published: January 2023

This paper presents five tests based on behavioural and other animal-centred observations concerning dairy goat welfare. An emotional reactivity test (n = 40) classified the animals into different groups according to their behaviour in response to fear-eliciting stimuli, and identified anxious animals. Movement parameters and behaviour, as well as quantitative (number of cries) and qualitative (pitch, intensity, length) sound parameters were recorded. A dominance test (n = 35) based on antagonistic reactions resulted in three hierarchical groups (subordinate, n = 9; intermediate, n = 6; and dominant, n = 20). A test performed in the milking parlour (n = 108) showed that the order of passage was strongly preserved and linked to limb pathology and dominance index. Finally, a lameness test (12.5% were lame) and a standing up test (8.5% had problems getting up) showed that these two parameters were highly correlated. After some simplifications, these tests could form a goat welfare evaluation method.
Measuring fearfulness of hens in commercial organic egg production
Authors: Hegelund L , Sørensen JT
Published: January 2023

To evaluate agreement between flock-based fear tests used in welfare assessments of laying hens in commercial organic farms, three tests were applied in 27 flocks of layers. Tests were performed at 35 and 55 weeks of age and were based on the concepts of ‘novel object’, ‘sudden sound’ and two different measures of ‘approaching human’. In general, agreements between tests were poor. The two measures based on ‘approaching human’ had the highest agreement, while the agreement between all other flock-based tests were lower. There was low agreement between testing at age 35 and 55 weeks.

In order to evaluate the degree to which the flock-based fear tests reflect individual hens' underlying fearfulness; tonic immobility (TI) tests were also applied on individual hens in eight flocks. There was large individual variation in the duration of tonic immobility, but also significant difference between flocks regarding mean duration of immobilisation. However there was no statistically significant association between TI-tests and flock-based tests. These results emphasise the need for careful consideration of choice of test stimuli for flock-based fear tests, and indicate that the investigated flock-based fear tests do not reflect the individual hens' underlying fearfulness.
Welfare assessment on Japanese dairy farms using the Animal Needs Index
Authors: Seo T , Date K , Daigo T , Kashiwamura F , Sato S
Published: January 2023

The Animal Needs Index (ANI) is used for on-farm assessment of welfare in Austria. However, no synthetic welfare assessment system exists in Japan. We performed a welfare assessment of Japanese dairy farms using the ANI scoring system. On 25 farms, 36 dairy herds were assessed using the ANI35L/2000-cattle survey. On 10 dairy farms, 16 herds were housed in free-stall barns. The other 20 herds of 15 dairy farms were housed in tie-stall barns. This study was conducted between December 2004 and January 2005. The ANI scores ranged from 6.0 to 40.0 points (mean 17.8 points). The four high ranking herds on ANI value were housed in free-stalls and allowed the use of outside areas. The 13 low ranking herds were housed in tie-stalls and were not allowed the use of outside areas. The free-stall herds had significantly higher ANI scores than tie-stall herds. The ANI scores of herds having access to outside areas were significantly higher than those of herds having no outside access. This scoring system might be useful for Japanese dairy farms. However, some assessment parameters contain subjective criteria such as cleanliness, floor slipperiness, and animal health. Further studies are necessary to improve the scoring system, in particular clarifying criteria related to those parameters.
Leveraging expertise in animal welfare to create educational equity
Authors: Siegford JM , Zanella AJ , Bernardo T , Heleski CR , Wickens CL , Laughlin K , Malinowski R
Published: January 2023

Globally, veterinarians and professionals in animal-related industries are faced with growing public concern for the welfare of animals, particularly those in production. To prepare professionals, courses in animal welfare should be created to provide consistent education on a global scale in an efficient and cost-effective manner. However, a creative approach to welfare education is needed to reduce the disparity between supply and demand for instruction in animal welfare. Michigan State University (MSU) is piloting a graduate-level, online interactive course in animal welfare assessment to provide students with high-quality science-based education from renowned international animal welfare experts at numerous institutions. Innovative use of technology enables students to interact with material, each other and instructors. Students actively apply their accumulated skills to hypothetical scenarios, enhancing learning effectiveness. Student performance and opinion were examined to determine whether the course meets the stated objectives. Twenty-three students from four institutions enrolled in the pilot course. In the first month, the majority of students found the online course easy to use and material appropriate for a graduate-level course. The students agreed that scenarios helped them integrate lecture material. The MSU course will serve as a model for collaboration in content assembly and course delivery, using technology to leverage global expertise to create educational equity.
Rank correlated use of soft flooring by dairy cattle
Authors: Platz S , Ahrens F , Bendel J , Meyer H , Erhard MH
Published: January 2023

The present study covers rank correlated use of soft flooring during partial replacement (45% of the total floor space) of concrete slatted floor by rubber covering. For this purpose, the rank position of 19 cows within a 50 Brown Swiss dairy cattle herd was evaluated by calculating the individual dominance index. A clear rank affiliation could be found for 16 cows (eight cows with a rank index 0.67 – 1.0 (ie high ranking) and eight cows with a rank index 0.0 – 0.25 (ie low ranking). Using focal sampling, the 16 cows were observed with time-lapse video recording before and after floor replacement (8.5 hours per day for 10 days with each floor kind). After replacing 45% of the concrete floor surface with soft flooring in a test area far from the milking parlour, high ranking cows used this area significantly longer ie 105.5 ± 36.2 min more than before compared with lower ranking herd members, who did so 17.6 ± 24.6 min less. Before replacement, low ranking cows spent significantly more time on the concrete slatted floor of the test area (147.6 ± 13.2 min) than high ranking cows (80.9 ± 17.3 min). The results of the observation demonstrate that the extent of use of soft rubber flooring correlates to rank-order and illustrates the importance of barn designs in terms of meeting their specific needs in spite of an existing rank order.
Quality of life and the evolution of the brain
Authors: Kendrick KM
Published: January 2023

The dual problem of explaining brain evolution and the way in which it has led to wide species differences in behaviour and physiology has often appeared intractable to scientists. The main limiting factor is that we do not understand enough about how brains work to appreciate why gross or fine morphological differences can lead to the considerable across- as well as within-species differences in behaviour. Even at a molecular level, while two-thirds of our genes are involved in regulating brain function, there is a high degree of homology within different phyla. In the context of quality of life (QoL), arguably the most important consideration is that the brain you have evolved is adapted to the environment you are living in and is capable of generating ‘conscious’ experience. When that environment is radically altered, issues arise regarding whether there is sufficient adaptability to cope and the extent to which mental as well as physical suffering might be experienced as a consequence. At the other end of the spectrum there is the question of how enriched social and physical environments might enhance QoL through promoting positive affect. Here I will discuss potential functional contributions of differences in brain size and organisation and the impact of experience. I will mainly focus on mental functioning and show particularly that capacities for consciousness, emotional experience, social interaction and cognition and behavioural flexibility are likely to be widespread in other animal species, even if less developed than in humans.
How animals communicate quality of life: the qualitative assessment of behaviour
Authors: Wemelsfelder F
Published: January 2023

The notion ‘quality of life’ (QoL) suggests that welfare in animals encompasses more than just an absence of suffering; it concerns the quality of an animal's entire relationship with its environment, of how it lives its life. Judgements of such quality are based on the integration of perceived details of how animals behave over time in different contexts. The scientific status of such judgements has long been ambiguous, but in recent decades has begun to be addressed by animal scientists. This paper starts with a brief review of qualitative approaches to the study of animal behaviour, which tend to address characteristics such as individuality, personality, and emotionality. The question then arises whether such characteristics involve a subjective, experiential aspect, and identify animals as sentient beings. The second half of this paper argues that taking the integrative nature of qualitative judgements seriously enables a ‘whole animal’ perspective, through which it becomes possible to view behaviour as a dynamic, expressive body language that provides a basis for assessing the quality of an animal's experience (eg contented, anxious). Judging this quality is a skill that requires knowledge of species-specific behaviour, experience in observing and interacting with animals in different contexts, and a willingness to communicate with animals as sentient beings. A substantial body of research indicates that this skill can function reliably in a scientific context, and can be applied usefully as a practical welfare assessment tool. Thus qualitative approaches to the study of animal behaviour should make an important contribution to the growing interest in animal QoL.