bluuebunny/crossref_metadata_2025 huggingface dataset:
Reconceptualising Behavioral and Psychological Symptoms of Dementia: Views of People Living With Dementia and Families/Care Partners
Authors: Burley Claire V. , Casey Anne-Nicole , Chenoweth Lynn , Brodaty Henry
Published: August 2021

Background: Behavioral and psychological symptoms of dementia (BPSD, also known as neuropsychiatric symptoms (NPS), changed behaviors and responsive behaviors), occur in up to 90 percent of people living with dementia (PLWD). These symptoms and behaviors strongly correlate with functional and cognitive impairment and contribute to ~30% of overall dementia costs. As decisions regarding care and strategies for BPSD are generally based on professional frames of reference, this study investigates whether the perspectives of PLWD and families/care partner on BPSD terminology can inform a more nuanced conceptualization of BPSD.

Methods: PLWD and families/care partners participated in one-on-one semi-structured interviews. A thematic iterative approach was used to code the data and identify common themes until theoretical saturation was reached. Themes were compared between groups. Data were analyzed deductively in relation to pre-existing terminology regarding BPSD, and inductively to discover new ideas on use of such terminology as perceived by PLWD and others.

Results: Forty-one volunteers were interviewed: 21 PLWD, mean age 71 yrs, mean Mini-Mental State Examination score 25, and 20 family members/care partners. Three main themes emerged from the data: (1) descriptions of BPSD from people with lived experience compared to clinical terms, (2) viewpoints on interpreting causes, and (3) experiences of concurrent BPSD. The experiences described and terms used by PLWD and families/care partners differed from terms used in existing professional frameworks (e.g., “disinhibition” described as ‘loss of filter') and there were differences between PLWD and family members' interpretations of BPSD causes.

Discussion/Conclusion: Reports from PLWD and families/carers describing their experiences of BPSD suggest a reconceptualization of BPSD terminology is needed to understand and de-stigmatize these symptoms and behaviors. For example, the term “agitated/hard to handle” would benefit by clearer, contextualized description, such as “frustrated with cognitive decline, discriminatory behavior and inadequate support systems.” In better understanding individual expressions of BPSD, families, professionals and societies will be able to respond in ways that are helpful for PLWD. An informed, integrated understanding of BPSD and improved terminology use will have the potential to improve the quality of care and support for PLWD.
Marsh Madness: Restoring Tidal Wetlands in Our Estuaries
Authors: Sherman Stacy , Hartman Rosemary
Published: August 2021

Just like people, fish need a safe place to find food and grow up. For fish that travel between fresh water and the ocean, tidal wetlands are a perfect neighborhood, with lots of habitat and food. Tidal wetlands are areas of shallow water where tides from the ocean cover the land with water every day. Besides providing a home for fish and water-loving plants, tidal wetlands also help protect people and their property from natural disasters like storms, and from sea-level rise. People have not always understood the value of wetlands, so billions of acres of them have been filled to farm or build on. In the San Francisco Estuary, more than 90% of wetlands were converted to other uses in <150 years! People now understand why wetlands are important, so protecting and restoring wetlands is a top priority.
Prevalence, Characterization, and Pathogenicity of Salmonella enterica Subspecies enterica Serovar Derby from Yaks in the Aba Tibetan Autonomous Prefecture, China
Authors: Fu Xue , Feng Lan , Kong Linghan , Li Chun , Zhao Xiaodong , Li Huade , Cui Pengfei , Yan Wenjun , Zhai Yaru , Zhang Lan , Li Hao , Wang Hongning , Yang Xin
Published: August 2021

Salmonella enterica subsp. enterica serovar Derby (S. Derby) is one of the numerous non-typhoidal Salmonella serovars and has been recognized as a food-borne pathogen. In 2019, outbreaks of salmonellosis were reported in 13 yak farms in the Aba Tibetan Autonomous Prefecture, China. A total of 32 salmonella strains were isolated from 162 fecal samples of yaks with diarrhea as well as from drinking water samples. The isolates were subjected to serovar identification, animal experiments, and whole-genome sequencing (WGS) analyses. The serovar of all the isolates was S. Derby, and the sequence types (STs) were ST40. The analysis of the differences of single-nucleotide polymorphisms (SNPs) showed that the salmonella strains isolated from 13 farms were clonally related. Animal experiments showed that the lethal dose (LD50) was 4.57 × 107 CFU (colony-forming units); the shedding time of S. Derby in mice was 24 days; the bacterial loads in spleen were higher than those in other organs (ileum, liver, and cecum). Pathological analyses by hematoxylin and eosin (H&E) staining revealed obvious damage in the spleen, liver, and intestine. These results indicate that the S. Derby from yaks can cause infection in mice.
A Novel Miniaturized Biosensor for Monitoring Atlantic Salmon Swimming Activity and Respiratory Frequency
Authors: Kolarevic Jelena , Calduch-Giner Josep , Espmark Åsa M. , Evensen Tor , Sosa Javier , Pérez-Sánchez Jaume
Published: August 2021

The advanced development of sensor technologies has led to the emergence of fish biosensors that are currently used for research and commercial purposes. AEFishBIT is a miniaturized biosensor attached to fish operculum that measures physical activity and respiration frequencies. In this study, we determined the effect of the tagging method and evaluated the use of this biosensor to monitor post-smolt Atlantic salmon in a tank-based system. The use of piercing fish tag had a negative impact on the gills and operculum, unlike the identical protocols used in gilthead sea bream and European sea bass. In contrast, a surgical thread did not show any apparent tissue damage. Two data recording schedules were considered to monitor immediate early reaction to fish handling and light regime changes (records every 15 min over 2 days) or adaptation to new light conditions (records every 30 min over 4 days). Data showed stabilization of physical activity 8 h post-tagging, with different steady states for the activity/respiratory ratio after changes in light intensity that reflected a different time course adaptation to new light conditions. High correlations were observed between AEFishBIT and video recording data. These findings supported the use of AEFishBIT as a promising tool for smart sensing of Atlantic salmon.
Pilot Investigation of Anti-Salmonella Antibodies in Oral Fluids from Salmonella Typhimurium Vaccinated and Unvaccinated Swine Herds
Authors: De Lucia Alessia , Cawthraw Shaun A. , Smith Richard Piers , Davies Rob , Bianco Carlo , Ostanello Fabio , Martelli Francesca
Published: August 2021

Oral fluid (OF) can be a simple, cheap and non-invasive alternative to serum or meat juice for the diagnosis and surveillance of important pathogens in pigs. This study was conducted on four Salmonella Typhimurium-positive farrow-to-finish pig farms: two Salmonella-vaccinated (V) and two non-vaccinated (NV). Gilts and sows in the V farms were vaccinated with a live, attenuated vaccine prior to farrowing. Pooled faecal and OF samples were collected from the sows and their offspring. Salmonella was isolated according to ISO6579–1:2017. In parallel, IgG and IgA levels were assessed in OF samples using a commercial ELISA assay. Salmonella was detected in 90.9% of the pooled faecal samples from the NV farms and in 35.1% of the pooled faecal samples from the V farms. Overall, a higher prevalence was observed in the pooled faecal samples from the offspring (76.3%) compared to the sows (36.4%). IgG antibodies measured in V farms are likely to be related to vaccination, as well as exposure to Salmonella field strains. The detection of IgA antibodies in OF was unreliable with the method used. The results of this study show that IgG is the most reliable isotype for monitoring Salmonella-specific antibody immunity in vaccinated/infected animals via OF.
Environmental Surveillance and Characterization of Antibiotic Resistant Staphylococcus aureus at Coastal Beaches and Rivers on the Island of Hawaiʻi
Authors: Gerken Tyler J. , Roberts Marilyn C. , Dykema Philip , Melly Geoff , Lucas Darren , De Los Santos Vanessa , Gonzalez Joenice , Butaye Patrick , Wiegner Tracy N.
Published: August 2021

Staphylococcus aureus are human facultative pathogenic bacteria and can be found as contaminants in the environment. The aim of our study was to determine whether methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) isolated from coastal beach and river waters, anchialine pools, sand, and wastewater on the island of Hawaiʻi, Hawaiʻi, are a potential health risk. Samples were collected from three regions on Hawaiʻi Island from July to December 2020 during the COVID-19 pandemic and were characterized using whole-genome sequencing (WGS). From WGS data, multilocus sequence typing (MLST), SCCmec type, antimicrobial resistance genes, virulence factors, and plasmids were identified. Of the 361 samples, 98.1% were positive for Staphylococcus spp. and 7.2% were S. aureus positive (n = 26); nine MRSA and 27 MSSA strains were characterized; multiple isolates were chosen from the same sample in two sand and seven coastal beach water samples. The nine MRSA isolates were multi-drug resistant (6–9 genes) sequence type (ST) 8, clonal complex (CC) 8, SCCmec type IVa (USA300 clone), and were clonally related (0–16 SNP differences), and carried 16–19 virulence factors. The 27 MSSA isolates were grouped into eight CCs and 12 STs. Seventy-eight percent of the MSSA isolates carried 1–5 different antibiotic resistance genes and carried 5–19 virulence factors. We found S. aureus in coastal beach and river waters, anchialine pools, and sand at locations with limited human activity on the island of Hawaiʻi. This may be a public health hazard.
Clinical Findings in SMR Neurofeedback Protocol Training in Women with Fibromyalgia Syndrome
Authors: Barbosa-Torres Carlos , Cubo-Delgado Sixto
Published: August 2021

Fibromyalgia is related to central sensitization syndrome (CSS) and is associated with chronic pain and a decrease in general health. The aim of this study was to explore how changes in brain patterns of female fibromyalgia patients are shaped by neurofeedback therapy and how it affects pain perception and general health. A quasi-experimental study with pre- and post-tests was carried out with 37 female fibromyalgia patients referred by the Pain Unit of the National Health Service of Spain. The method involved applying a sensorimotor rhythm (SMR) protocol to monitor changes in brain waves under different conditions, taking pre-/post-test measurements of perceived pain, general health and the impact on fibromyalgia. Measures included the Fibromyalgia Impact Questionnaire Revised (FIQR), the Visual Analogue Scale (VAS), the General Health Questionnaire (GHQ-28) and EEG (SMR, theta waves). During therapy, the SMR/theta wave ratio increased significantly and after application of therapy, significant results were observed for the FIQR, VAS and GHQ-28. In conclusion, neurofeedback therapy increases the SMR/theta wave ratio in fibromyalgia, helping to maintain a balance between brain functions. This is associated with the activation of inhibitory processes, which is related to the perceived improvement of pain in fibromyalgia patients.
The Impact of Induction Regimes on Immune Responses in Patients with Multiple Myeloma
Authors: Firer Michael A. , Shapira Michael Y. , Luboshits Galia
Published: August 2021

Current standard frontline therapy for newly diagnosed patients with multiple myeloma (NDMM) involves induction therapy, autologous stem cell transplantation (ASCT), and maintenance therapy. Major efforts are underway to understand the biological and the clinical impacts of each stage of the treatment protocols on overall survival statistics. The most routinely used drugs in the pre-ASCT “induction” regime have different mechanisms of action and are employed either as monotherapies or in various combinations. Aside from their direct effects on cancer cell mortality, these drugs are also known to have varying effects on immune cell functionality. The question remains as to how induction therapy impacts post-ASCT immune reconstitution and anti-tumor immune responses. This review provides an update on the known immune effects of melphalan, dexamethasone, lenalidomide, and bortezomib commonly used in the induction phase of MM therapy. By analyzing the actions of each individual drug on the immune system, we suggest it might be possible to leverage their effects to rationally devise more effective induction regimes. Given the genetic heterogeneity between myeloma patients, it may also be possible to identify subgroups of patients for whom particular induction drug combinations would be more appropriate.
Experimental and Clinical Evidence Supports the Use of Urokinase Plasminogen Activation System Components as Clinically Relevant Biomarkers in Gastroesophageal Adenocarcinoma
Authors: Tincknell Gary , Piper Ann-Katrin , Aghmesheh Morteza , Becker Therese , Vine Kara Lea , Brungs Daniel , Ranson Marie
Published: August 2021

Gastric and oesophageal cancers (GOCs) are lethal cancers which metastasise early and recur frequently, even after definitive surgery. The urokinase plasminogen activator system (uPAS) is strongly implicated in the invasion and metastasis of many aggressive tumours including GOCs. Urokinase plasminogen activator (uPA) interaction with its receptor, urokinase plasminogen activator receptor (uPAR), leads to proteolytic activation of plasminogen to plasmin, a broad-spectrum protease which enables tumour cell invasion and dissemination to distant sites. uPA, uPAR and the plasminogen activator inhibitor type 1 (PAI-1) are overexpressed in some GOCs. Accumulating evidence points to a causal role of activated receptor tyrosine kinase pathways enhancing uPAS expression in GOCs. Expression of these components are associated with poorer clinicopathological features and patient survival. Stromal cells, including tumour-associated macrophages and myofibroblasts, also express the key uPAS proteins, supporting the argument of stromal involvement in GOC progression and adverse effect on patient survival. uPAS proteins can be detected on circulating leucocytes, circulating tumour cells and within the serum; all have the potential to be developed into circulating biomarkers of GOC. Herein, we review the experimental and clinical evidence supporting uPAS expression as clinical biomarker in GOC, with the goal of developing targeted therapeutics against the uPAS.
Clinicopathological and Prognostic Significance of Inhibitor of Apoptosis Protein (IAP) Family Members in Lung Cancer: A Meta-Analysis
Authors: Fung Stephen , Knoefel Wolfram Trudo , Krieg Andreas
Published: August 2021

Lung cancer is the most common cause of cancer-related death worldwide. Approximately 85% is non-small-cell and 15% is small-cell lung cancer. The inhibitor of apoptosis proteins (IAPs) represent a heterogeneous family of anti-apoptotic proteins, some members of which have been reported to correlate with clinical outcome in lung cancer. We screened PubMed, Web of Science, and Scopus for studies that investigated the prognostic value and clinicopathological features of IAPs in lung cancer. Forty-five eligible studies with 4428 patients assessed the expression of the IAPs survivin, XIAP, livin, and BRUCE. The pooled hazard ratio (HR) of 33 studies that analyzed overall survival (OS) revealed a positive correlation between survivin expression and poor prognosis. Seven studies displayed a strong association between survivin and disease recurrence. Two studies that assessed the expression of XIAP and livin, respectively, proved a significant relationship of these IAPs with poor OS. Meta-analyses of clinicopathological variables revealed a significant association between survivin and T stage, UICC stage, the presence of lymph node metastasis, and grade of differentiation. In conclusion, high expression of distinct IAPs significantly correlates with prognosis in lung cancer. Therefore, lung cancer patients might benefit from a targeted therapy against specific IAPs.
Molecular Changes Induced in Melanoma by Cell Culturing in 3D Alginate Hydrogels
Authors: Kappelmann-Fenzl Melanie , Schmidt Sonja K. , Fischer Stefan , Schmid Rafael , Lämmerhirt Lisa , Fischer Lena , Schrüfer Stefan , Thievessen Ingo , Schubert Dirk W. , Matthies Alexander , Detsch Rainer , Boccaccini Aldo R. , Arkudas Andreas , Kengelbach-Weigand Annika , Bosserhoff Anja K.
Published: August 2021

Alginate hydrogels have been used as a biomaterial for 3D culturing for several years. Here, gene expression patterns in melanoma cells cultivated in 3D alginate are compared to 2D cultures. It is well-known that 2D cell culture is not resembling the complex in vivo situation well. However, the use of very intricate 3D models does not allow performing high-throughput screening and analysis is highly complex. 3D cell culture strategies in hydrogels will better mimic the in vivo situation while they maintain feasibility for large-scale analysis. As alginate is an easy-to-use material and due to its favorable properties, it is commonly applied as a bioink component in the growing field of cell encapsulation and biofabrication. Yet, only a little information about the transcriptome in 3D cultures in hydrogels like alginate is available. In this study, changes in the transcriptome based on RNA-Seq data by cultivating melanoma cells in 3D alginate are analyzed and reveal marked changes compared to cells cultured on usual 2D tissue culture plastic. Deregulated genes represent valuable cues to signaling pathways and molecules affected by the culture method. Using this as a model system for tumor cell plasticity and heterogeneity, EGR1 is determined to play an important role in melanoma progression.
Interaction of Fibromodulin and Myostatin to Regulate Skeletal Muscle Aging: An Opposite Regulation in Muscle Aging, Diabetes, and Intracellular Lipid Accumulation
Authors: Lee Eun Ju , Ahmad Syed Sayeed , Lim Jeong Ho , Ahmad Khurshid , Shaikh Sibhghatulla , Lee Yun-Sil , Park Sang Joon , Jin Jun O. , Lee Yong-Ho , Choi Inho
Published: August 2021

The objective of this study was to investigate fibromodulin (FMOD) and myostatin (MSTN) gene expressions during skeletal muscle aging and to understand their involvements in this process. The expressions of genes related to muscle aging (Atrogin 1 and Glb1), diabetes (RAGE and CD163), and lipid accumulation (CD36 and PPARγ) and those of FMOD and MSTN were examined in CTX-injected, aged, MSTN−/−, and high-fat diet (HFD) mice and in C2C12 myoblasts treated with ceramide or grown under adipogenic conditions. Results from CTX-injected mice and gene knockdown experiments in C2C12 cells suggested the involvement of FMOD during muscle regeneration and myoblast proliferation and differentiation. Downregulation of the FMOD gene in MSTN−/− mice, and MSTN upregulation and FMOD downregulation in FMOD and MSTN knockdown C2C12 cells, respectively, during their differentiation, suggested FMOD negatively regulates MSTN gene expression, and MSTN positively regulates FMOD gene expression. The results of our in vivo and in vitro experiments indicate FMOD inhibits muscle aging by negatively regulating MSTN gene expression or by suppressing the action of MSTN protein, and that MSTN promotes muscle aging by positively regulating the expressions of Atrogin1, CD36, and PPARγ genes in muscle.
Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies
Authors: Hoppel Florian , Garcia-Souza Luiz Felipe , Kantner-Rumplmair Wilhelm , Burtscher Martin , Gnaiger Erich , Pesta Dominik , Calabria Elisa
Published: August 2021

Human blood cells may offer a minimally invasive strategy to study systemic alterations of mitochondrial function. Here we tested the reliability of a protocol designed to study mitochondrial respiratory control in human platelets (PLTs) in field studies, using high-resolution respirometry (HRR). Several factors may trigger PLT aggregation during the assay, altering the homogeneity of the cell suspension and distorting the number of cells added to the two chambers (A, B) of the Oroboros Oxygraph-2k (O2k). Thus, inter-chamber variability (∆ab) was calculated by normalizing oxygen consumption to chamber volume (JO2) or to a specific respiratory control state (flux control ratio, FCR) as a reliable parameter of experimental quality. The method’s reliability was tested by comparing the ∆ab of laboratory-performed experiments (LAB, N = 9) to those of an ultramarathon field study (three sampling time-points: before competition (PRE, N = 7), immediately after (POST, N = 10) and 24 h after competition (REC; N = 10)). Our results show that ∆ab JO2 changed PRE-POST, but also for LAB-POST and LAB-REC, while all ∆ab FCR remained unchanged. Thus, we conclude that our method is reliable for assessing PLT mitochondrial function in LAB and field studies and after systemic stress conditions.
Comprehensive Integrative Analysis Reveals the Association of KLF4 with Macrophage Infiltration and Polarization in Lung Cancer Microenvironment
Authors: Arora Shweta , Singh Prithvi , Ahmad Shaniya , Ahmad Tanveer , Dohare Ravins , Almatroodi Saleh A. , Alrumaihi Faris , Rahmani Arshad Husain , Syed Mansoor Ali
Published: August 2021

Macrophage polarization and infiltration to the tumor microenvironment (TME) is a critical determining factor for tumor progression. Macrophages are polarized into two states—M1 (pro-inflammatory, anti-tumorigenic and stimulated by LPS or IFN-γ) and M2 (anti-inflammatory pro-tumorigenic and stimulated by IL-4) phenotypes. Specifically, M2 macrophages enhance tumor cell growth and survival. Recent evidences suggest the pivotal role of microRNAs in macrophage polarization during the development of Non-small cell lung cancer (NSCLC), thus proposing a new therapeutic option to target lung cancer. In silico analysis determined cogent upregulation of KLF4, downregulation of IL-1β and miR-34a-5p in NSCLC tissues, consequently worsening the overall survival of NSCLC patients. We observed a significant association of KLF4 with macrophage infiltration and polarization in NSCLC. We found that KLF4 is critically implicated in M2 polarization of macrophages, which, in turn, promotes tumorigenesis. KLF4 expression correlated with miR-34a-5p and IL-1β in a feed-forward loop (FFL), both of which are implicated in immune regulation. Mechanistic overexpression of miR-34a-5p in macrophages (IL-4 stimulated) inhibits KLF4, along with downregulation of ARG1, REL-1MB (M2 macrophage specific markers), and upregulation of IL-1β, IL-6, (M1 macrophage specific markers), demonstrating macrophage polarization switch from M2 to M1 phenotype. Moreover, co-culture of these macrophages with NSCLC cells reduces their proliferation, wound healing, clonogenic capacity and enhanced NO-mediated apoptosis. Further, transfection of miR-34a-5p in NSCLC cells, also degrades KLF4, but enhances the expression of KLF4 regulated genes—IL-1β, IL-6 (pro-inflammatory mediators), which is further enhanced upon co-culture with IL-4 stimulated macrophages. Additionally, we observed a significant increase in i-NOS/NO content upon co-culture, suggesting polarization reversion of macrophages from M2 to M1, and eventually leading to anti-tumor effects. Our findings thus show a significant role of KLF4 in tumorigenesis and TAM polarization of NSCLC. However, miR-34a-5p mediated targeting of these molecular networks will provide a better therapeutic intervention for NSCLC.
The Influence of Battery Acid on the Destruction of Acrylic Coatings of Car Bodies
Authors: Kotnarowska Danuta
Published: August 2021

Renovation coatings of car bodies undergo destruction under the influence of operational factors. Like ultraviolet radiation, erosion, and aggressive media (among others, battery acid). This article concerns the evaluation of the influence of battery acid on the destruction of acrylic coatings previously non-aged, as well as aged climatically for 2 years. Ageing of the coatings with battery acid contributed to a degradation increase of their chemical structure. It was supported by a considerable increase in the polar component of surface free energy (SFE) of the coatings. In the case of prior climate ageing, the increase in the polar component was even higher. Moreover, the coating’s ability to absorb battery acid increased, which induced blistering. The DSC method revealed that the action of battery acid caused more intense oxidation of coating material, and as a result, the brittleness increased, leading to chipping of the coating surface layers. This led to the increase in surface roughness, measured using an interferometric method. The coatings previously climatically aged for 2 years presented higher values of surface roughness parameters than the non-aged ones. The increase in the surface roughness contributed to a substantial decrease in the gloss of coatings. A sharp difference in colour escalating with the lengthening of the ageing period was also observed using the spectrophotometric method.
The Local Distribution of Temperatures and Entropy Generation Rate in an Ideal Counterflow Heat Exchanger
Authors: Dong Zhimin , Du Qinglin
Published: August 2021

The process of heat exchange between two fluids of different temperatures and separated by a solid wall occurs in many engineering applications. Log mean temperature difference and effectiveness-NTU methods are widely used to assist in the design of heat exchangers. However, the two methods focus on overall analysis and cannot show the local temperature distributions. This paper obtains the mathematical solutions to the temperature profiles in an ideal counterflow heat exchanger. The aim of this research is to explain the phenomenon called the “entropy generation paradox”, which indicates a discrepancy between effectiveness and optimal entropy generation. The theoretical analysis reveals that the temperature curves are exponential functions when the heat capacity rates of the two streams are different; otherwise, the curves are linear functions. A heat exchanger is demonstrated to draw the temperature profiles under different working conditions. Local entropy generation rates are determined by the ratio of local stream temperatures in the form of a hook function. To realize a certain heat duty, there are many stream flow rate couples, and each couple results in a different entropy generation profile and obtains a corresponding total entropy generation. The helical steam generator of a high-temperature gas-cooled reactor is analyzed in this article and the principle of equipartition of entropy generation is confirmed. This principle indicates that, among the many working conditions to achieve a certain heat duty, a heat exchanger characterized by a nearly constant entropy production gives the best second law efficiency possible in order to achieve the best energy conversion.
Unraveling the Biosynthesis of Quinolizidine Alkaloids Using the Genetic and Chemical Diversity of Mexican Lupins
Authors: Ramírez-Betancourt Astrid , Hernández-Sánchez Arianna Michelle , Salcedo-Morales Guadalupe , Ventura-Zapata Elsa , Robledo Norma , Wink Michael , Bermúdez-Torres Kalina
Published: August 2021

Quinolizidine alkaloids (QAs) are synthesized by the genus Lupinus as a defense against herbivores. Synthesis of QAs in lupins is species- and organ-specific. Knowledge about their biosynthesis and their corresponding pathways are still fragmentary, in part because lupins of commercial importance were mainly investigated, representing a small sample of the chemodiversity of the genus. Here, we explore the use of three Mexican lupins: Lupinus aschenbornii, Lupinus montanus, and Lupinus bilineatus as a model to study the physiology of QA biosynthesis. The corresponding QA patterns cover widely and narrowly distributed tetracyclic QAs. Quinolizidine alkaloid patterns of seeds and plantlets at different developmental stages were determined by GLC–MS and compared to identify the onset of de novo QA synthesis and to gain insight into specific and common biosynthesis trends. Onset of de novo QA biosynthesis occurred after the metabolization of seed QA during germination and was species-specific, as expected. A common QA pattern, from which the diversity of QA observed in these species is generated, was not found; however, lupanine and 3β-lupanine were found in the three specieswhile sparteine was not found in Lupinus bilineatus, suggesting that this simplest tetracyclic QA is not the precursor of more complex QAs. Similar patterns of metabolization and biosynthesis of structurally related QAs were observed, suggesting a common regulation.
Complex Component of Oncocytic and Non-Oncocytic Lipoadenomas in the Parotid Gland: A Case Report
Authors: Sato Fuyuki , Nakajima Takashi , Sugino Takashi
Published: August 2021

Oncocytic lipoadenoma of the salivary gland is a rare tumor that develops mainly in the parotid gland. We report a case of oncocytic lipoadenoma of the parotid gland in a 70-year-old woman. The tumor measured 30 × 20 mm and had a well-circumscribed tan-brown surface. The tumor was histologically composed of oncocytic and lipomatous lesions without atypia. In addition to the oncocytic lipoadenoma, a small lipomatous tumor, measuring 10 × 7 mm, was found in the resected parotid gland. Macroscopically, this tumor was yellow and indistinguishable from the parotid gland. Microscopically, the tumor was rich in fats and contained an area of conglomerated duct-like proliferation and salivary gland components. Therefore, the tumor was diagnosed as a non-oncocytic lipoadenoma with a sialoadenoma component. We report the first case of double component oncocytic and non-oncocytic lipoadenomas of the salivary gland.
Biometric Identification Systems with Noisy Enrollment for Gaussian Sources and Channels
Authors: Yachongka Vamoua , Yagi Hideki , Oohama Yasutada
Published: August 2021

In the present paper, we investigate the fundamental trade-off of identification, secret-key, storage, and privacy-leakage rates in biometric identification systems for remote or hidden Gaussian sources. We use a technique of converting the system to one where the data flow is in one-way direction to derive the capacity region of these rates. Also, we provide numerical calculations of three different examples for the system. The numerical results imply that it seems hard to achieve both high secret-key and small privacy-leakage rates simultaneously.
Hold-Up Problems in International Gas Trade: A Case Study
Authors: Nuryyev Guych , Korol Tomasz , Tetin Ilia
Published: August 2021

The infrastructure required for international natural gas trade is considerable, which often leads to hold-up problems and supply disruptions. This study discusses disruptions of gas supply from Algeria, Indonesia, Russia, and Turkmenistan since the early 1980s. The novelty of this study is its focus on the issues related to transit countries, which are rarely considered in the literature. The results of the study classify supply disruptions into six types, show the evolution of supply disruptions over time, and discuss mitigation strategies. The six types of disruptions include political change, price demands, debts, technical issues, transit fees, theft of gas. The evolution of the disruptions shows that the issues related to transit countries have become more frequent in the last two decades. Mitigation strategies tailored to transit countries include using an international organisation, designing contracts with price mechanisms that might reduce the possibility of disputes and reducing the number of parties involved in the trade.
Control Strategy of 1 kV Hybrid Active Power Filter for Mining Applications
Authors: Buła Dawid , Michalak Jarosław , Zygmanowski Marcin , Adrikowski Tomasz , Jarek Grzegorz , Jeleń Michał
Published: August 2021

The paper presents a shunt hybrid active power filter for 1 kV mining applications in configuration with a single-tuned passive filter. The focus is on the method of controlling the filter, with particular emphasis on the influence of network voltage distortion and time delays in control on the effectiveness of harmonic reduction, which is one of the most important aspect of power quality. The low-power loss configuration of a hybrid filter with SiC transistors is shown, as well as the control algorithm which limits the influence of voltage distortion. Theoretical considerations are verified by results obtained from simulations and tests of the hybrid active power filter prototype.
The Use of 3D Numerical Modeling in Conceptual Design: A Case Study
Authors: Michalak Hanna , Przybysz Paweł
Published: August 2021

This article describes the construction of a building with four aboveground floors and one underground floor as part of the ongoing development of Warsaw’s city center. A 3D numerical model was developed to reflect the spatial and structural solutions of the new building based on the design documentation with regard to the outcomes of geotechnical tests, the actual phases of work completed, the results of the geodetic measurements carried out in individual phases of the building implementation, and the characteristics of the existing adjacent buildings. The 3D numerical model was calibrated taking into account the results of the geodetic measurements of the benchmarks stabilized on the adjacent buildings. The numerical models of the building were used to analyze a number of multiple-step variants, taking into account the increase in the number of aboveground floors (from 1 to 4) and underground floors (by 1), as well as the increase in the projected area of the underground part compared to the area of the site designated for development. The paper presents the conclusions of our analyses, which may be helpful to others designing buildings in intensively urbanized areas and guide them in selecting the best solution.
Generalized Peukert Equations Use for Finding the Remaining Capacity of Lithium-Ion Cells of Any Format
Authors: Yazvinskaya Nataliya N. , Galushkin Nikolay E. , Ruslyakov Dmitriy V. , Galushkin Dmitriy N.
Published: August 2021

In many studies, for predicting the remaining capacity of batteries belonging to different electrochemical systems, various analytical models based on the Peukert equation are used. This paper evaluates the advantages and disadvantages of the most famous generalized Peukert equations. For lithium-ion batteries, the Peukert equation cannot be used for estimation of their remaining capacity over the entire range of discharge currents. However, this paper proves that the generalized Peukert equations enable estimation of the capacity released by lithium-ion batteries with high accuracy. Special attention is paid to two generalized Peukert equations: C = Cm/(1 + (i/i0)n) and C = Cmerfc((i-i0)/n))/erfc(-i0/n). It is shown that they correspond to the experimental data the best.
Electrodeposited Copper Nanocatalysts for CO2 Electroreduction: Effect of Electrodeposition Conditions on Catalysts’ Morphology and Selectivity
Authors: Zanellato Gianluca , Schiavi Pier Giorgio , Zanoni Robertino , Rubino Antonio , Altimari Pietro , Pagnanelli Francesca
Published: August 2021

Catalytic electroreduction of carbon dioxide represents a promising technology both to reduce CO2 emissions and to store electrical energy from discontinuous sources. In this work, electrochemical deposition of copper on to a gas-diffusion support was tested as a scalable and versatile nanosynthesis technique for the production of catalytic electrodes for CO2 electroreduction. The effect of deposition current density and additives (DAT, DTAB, PEG) on the catalysts’ structure was evaluated. The selectivity of the synthesized catalysts towards the production of CO was evaluated by analyzing the gaseous products obtained using the catalysts as cathodes in electroreduction tests. Catalyst morphology was deeply influenced by the deposition additives. Copper nanospheres, hemispherical microaggregates of nanowires, and shapeless structures were electrodeposited in the presence of dodecyltrimethylammonium bromide (DTAB), 3,5-diamino-1,2,4-triazole (DAT) and polyethylene glycol (PEG), respectively. The effect of the deposition current density on catalyst morphology was also observed and it was found to be additive-specific. DTAB nanostructured electrodes showed the highest selectivity towards CO production, probably attributable to a higher specific surface area. EDX and XPS analysis disclosed the presence of residual DAT and DTAB uniformly distributed onto the catalysts structure. No significant effects of electrodeposition current density and Cu(I)/Cu(II) ratio on the selectivity towards CO were found. In particular, DTAB and DAT electrodes yielded comparable selectivity, although they were characterized by the highest and lowest Cu(I)/Cu(II) ratio, respectively.
Red Oak (Quercus rubra L.) Fruits as Potential Alternative for Cocoa Powder: Optimization of Roasting Conditions, Antioxidant, and Biological Properties
Authors: Marc (Vlaic) Romina Alina , Niculae Mihaela , Páll Emőke , Mureșan Vlad , Mureșan Andruţa , Tanislav Anda , Pușcaș Andreea , Mureșan Crina Carmen , Cerbu Constantin
Published: August 2021

Cocoa powder is a basic ingredient in the manufacture of chocolate, one of the most appreciated sweet products in the world for its sensory and nutritional properties. Furthermore, it displays a central nervous system stimulant effect. This study aimed to investigate acorn-derived powder as an alternative to cocoa powder, in order to obtain a chocolate that does not contain stimulants of the nervous system. Both the chocolate technological process and acorns roasting process (180 °C/25 min, 200 °C/20 min and 220 °C/15 min) were optimized to obtain acorn powder with an organoleptic profile as close as possible to that of cocoa powder. The chocolate sensory evaluation was performed by means of the hedonic test. Furthermore, the aqueous extracts obtained from the resulting powder were evaluated for total polyphenol content, and in vitro antimicrobial and antiproliferative properties. The results point out a high content of phenolic compounds (500.78–524.01 mg GAE/100 g); protection against microbial contamination based on the ability to inhibit a Gram-positive bacterium (Bacillus cereus) was also noticed. The aqueous acorn extracts were also able to reduce the cell viability of HFL-1 (human fetal lung fibroblast) and DLD-1 (colorectal adenocarcinoma) lines. This study suggests red oak (Quercus rubra) fruits as a potential alternative to cocoa powder in the manufacture of chocolate.
Effect of Feller-Buncher Model, Slope Class and Cutting Area on the Productivity and Costs of Whole Tree Harvesting in Brazilian Eucalyptus Stands
Authors: Miyajima Ricardo Hideaki , Fenner Paulo Torres , Batistela Gislaine Cristina , Simões Danilo
Published: August 2021

The operational productivity and costs of tree felling operations can be influenced by several factors, among which, the machine characteristics, slope class, the cutting area and the individual volume of the trees stand out. Thus, the main objective of the present study was to analyze the productivity and production cost for two feller-bunchers with different technical characteristics operating in a eucalyptus forest. The productivity was calculated from a time study and the factors analyzed were two feller-buncher models, two slope classes, and two cutting areas. The machine cost per scheduled hour was based on the methodology of the Food and Agriculture Organization of the United Nations. Analysis of the results showed that the felling and turn operational elements occupied the most time in the operational cycle of feller-bunchers. The machine cost per scheduled hour was USD 69.69 h−1 for feller-buncher 1 and USD 102.03 h−1 for feller-buncher 2. In conclusion, the distinct technical characteristics of feller-bunchers were found to influence the productivity and, consequently, the cost, of the felling operation during the harvesting of whole eucalyptus trees.
Transcriptome Analysis of Post-Mortem Brain Tissue Reveals Up-Regulation of the Complement Cascade in a Subgroup of Schizophrenia Patients
Authors: Lindholm Carlström Eva , Niazi Adnan , Etemadikhah Mitra , Halvardson Jonatan , Enroth Stefan , Stockmeier Craig A. , Rajkowska Grazyna , Nilsson Bo , Feuk Lars
Published: August 2021

Schizophrenia is a genetically complex neuropsychiatric disorder with largely unresolved mechanisms of pathology. Identification of genes and pathways associated with schizophrenia is important for understanding the development, progression and treatment of schizophrenia. In this study, pathways associated with schizophrenia were explored at the level of gene expression. The study included post-mortem brain tissue samples from 68 schizophrenia patients and 44 age and sex-matched control subjects. Whole transcriptome poly-A selected paired-end RNA sequencing was performed on tissue from the prefrontal cortex and orbitofrontal cortex. RNA expression differences were detected between case and control individuals, focusing both on single genes and pathways. The results were validated with RT-qPCR. Significant differential expression between patient and controls groups was found for 71 genes. Gene ontology analysis of differentially expressed genes revealed an up-regulation of multiple genes in immune response among the patients (corrected p-value = 0.004). Several genes in the category belong to the complement system, including C1R, C1S, C7, FCN3, SERPING1, C4A and CFI. The increased complement expression is primarily driven by a subgroup of patients with increased expression of immune/inflammatory response genes, pointing to important differences in disease etiology within the patient group. Weighted gene co-expression network analysis highlighted networks associated with both synaptic transmission and activation of the immune response. Our results demonstrate the importance of immune-related pathways in schizophrenia and provide evidence for elevated expression of the complement cascade as an important pathway in schizophrenia pathology.
Genome-Wide Analysis for Early Growth-Related Traits of the Locally Adapted Egyptian Barki Sheep
Authors: Abousoliman Ibrahim , Reyer Henry , Oster Michael , Murani Eduard , Mohamed Ismail , Wimmers Klaus
Published: August 2021

Sheep play a critical role in the agricultural and livestock sector in Egypt. For sheep meat production, growth traits such as birth and weaning weights are very important and determine the supply and income of local farmers. The Barki sheep originates from the northeastern coastal zone of Africa, and due to its good adaptation to the harsh environmental conditions, it contributes significantly to the meat production in these semi-arid regions. This study aimed to use a genome-wide SNP panel to identify genomic regions that are diversified between groups of individuals of Egyptian Barki sheep with high and low growth performance traits. In this context, from a phenotyped population of 140 lambs of Barki sheep, 69 lambs were considered for a genome-wide scan with the Illumina OvineSNP50 V2 BeadChip. The selected lambs were grouped into divergent subsets with significantly different performance for birth weight and weaning weight. After quality control, 63 animals and 40,383 SNPs were used for analysis. The fixation index (FST) for each SNP was calculated between the groups. The results verified genomic regions harboring some previously proposed candidate genes for traits related to body growth, i.e., EYA2, GDF2, GDF10, MEF2B, SLC16A7, TBX15, TFAP2B, and TNNC2. Moreover, novel candidate genes were proposed with known functional implications on growth processes such as CPXM2 and LRIG3. Subsequent association analysis showed significant effects of the considered SNPs on birth and weaning weights. Results highlight the genetic diversity associated with performance traits and thus the potential to improve growth traits in the Barki sheep breed.
Psychological Impact of the COVID-19 Pandemic on Licensed Full-Time Practicing Nurses Undertaking Part-Time Studies in Higher Education: A Cross-Sectional Study
Authors: Chan Siu-Ling , Takemura Naomi , Chau Pui-Hing , Lin Chia-Chin , Wang Man-Ping
Published: August 2021

Frontline nurses face an unpreceded situation with the coronavirus disease (COVID-19) pandemic, and many report suffering from physical and psychological stress. This online, cross-sectional survey used questionnaires, such as the Generalized Anxiety Disorder (GAD-7) questionnaire, the Patient Health Questionnaire (PHQ-2), the Connor–Davidson Resilience Scale, stress-related questions, and Brief Coping Orientation to Problems Experienced (Brief-COPE), to determine the psychological impact of COVID-19 on licensed full-time practicing nurses undertaking part-time studies in higher education. Recruitment commenced from August to September 2020; 385 students were approached, and 124 completed the survey (response rate: 32%). Most of the respondents were frontline nurses working in public sectors (89.5%), 29% of whom reported symptoms of depression, and 61.3% reported mild to severe levels of anxiety. The GAD-7 was significantly associated with the resilience score (β = −0.188; p = 0.008) and exhaustion (β = 0.612; p < 0.001). The PHQ-2 was significantly associated with ‘anxiety about infection’ (β = 0.071; p = 0.048). A lower anxiety level was significantly associated with a higher resilience level and a lower level of exhaustion, and a lower depression level was significantly associated with a lower anxiety about infection. Nursing programs incorporating resilience building may mitigate psychological distress of the study population.
Knowledge, Experience and Perception of Gender-Based Violence Health Services: A Mixed Methods Study on Adolescent Girls and Young Women in Tanzania
Authors: Mtaita Caroline , Likindikoki Samuel , McGowan Maureen , Mpembeni Rose , Safary Elvis , Jahn Albrecht
Published: August 2021

Many adolescent girls and young women (AGYW) experience gender-based violence (GBV) in Tanzania and only few seek GBV health services following violence. The objectives of our study are (1) to evaluate knowledge of gender-based violence among AGYW, (2) to explore their perceptions of and experiences with GBV health service quality and (3) to evaluate access to comprehensive GBV services. This study employed an explanatory, sequential mixed methods design in two districts of Dar es Salaam, Tanzania (Kinondoni and Temeke). A quantitative cross-sectional survey among AGYW (n = 403) between 15–24 years old was performed to assess their knowledge of GBV as well as perceptions of and experiences with GBV health services. The quantitative data was complemented by 20 semi-structured in-depth interviews with participants. Out of 403 study participants, more than three quarters (77.9%) had moderate to good knowledge of how GBV is defined and what constitutes gender-based violence. However, few participants (30.7%, n = 124) demonstrated knowledge of GBV health services offered at local health facilities. For example, only 10.7% (n = 43) of participants reported knowledge of forensic evidence collection. Additionally, of 374 participants (93% of total participants) who reported to have received GBV education sessions, only 66% accessed GBV health services (n = 247) and about half of these (52.7%, n = 130) were satisfied with these services. The study indicated that—despite good knowledge about what constituted GBV—knowledge about the roles and availability of GBV health services was limited and utilization of GBV health services remained low. Coordinated actions need to be strengthened to reach AGYW who remain unaware of GBV health services offered at health facilities by improving GBV service quality, bettering interventions aimed at reducing GBV among AGYW in Tanzania, and scaling-up integrated service models, such as GBV one-stop centers.