bluuebunny/crossref_metadata_2025 huggingface dataset:
Laser Additively Manufactured Magnetic Core Design and Process for Electrical Machine Applications
Authors: Tiismus Hans , Kallaste Ants , Vaimann Toomas , Lind Liina , Virro Indrek , Rassõlkin Anton , Dedova Tatjana
Published: May 2022

Additive manufacturing (AM) is considered the enabling technology for topology optimized components, with its unparalleled, almost free-form design freedom. Over the past decade, AM of electromagnetic materials has evolved into a promising new area of research. Considerable efforts have also been invested by the electrical machine (EM) research community to develop and integrate novel additive components. Several challenges remain, however, in printing soft magnetic flux guides—most prominently, reducing the induced eddy currents to achieve competitive AM core efficiency. This paper demonstrates the workflow of laser additive manufacturing magnetic cores with superior magnetic properties to soft magnetic composites (at 50 Hz excitation): describing the workflow, parameter tuning for both printing and annealing, and shape optimization. Process optimization yielded the optimal energy density of 77 J/mm3 and annealing temperature of 1200 °C, applied to prepare the samples with the highest relative density (99.86%), lowest surface roughness Rz (0.041 mm), minimal hysteresis losses (0.8 W/kg at 1.0 T, 50 Hz), and ultimate yield strength of 420 MPa. For Eddy current suppression, the sample (5 × 5 × 60 mm toroid) with bi-directional grading reached specific core losses as low as 1.8 W/kg (W10,50). Based on the findings, the advantages and disadvantages of AM graded cores are discussed in detail.
Landowner Acceptability of Silvicultural Treatments to Restore an Open Forest Landscape
Authors: Arnberger Arne , Gobster Paul H. , Schneider Ingrid E. , Floress Kristin M. , Haines Anna L. , Eder Renate
Published: May 2022

This study examined the acceptability of different silvicultural treatments to restore pine barrens, an open, fire-dependent forest landscape type globally imperiled across the northern Great Lakes region of the United States and Canada. In an online survey, we asked family-forest owners (N = 466) in Northeastern Wisconsin about the acceptability of pine barrens restoration treatments through ratings of both verbal descriptions and visual scenarios. An informational statement about pine barrens restoration purposes and goals preceded ratings for half the sample. Across the entire sample, acceptability ratings for eleven verbally-described treatments generally declined as treatments became more intensive, creating greater openness on the landscape. Information recipients found two groups of treatments identified by factor analysis (selective openings, fire) more acceptable than non-recipients, and cluster analysis identified four respondent subgroups, each with varying levels of acceptability. The respondents also rated the acceptability of visual scenarios, with treatment attribute combinations portraying a range of likely restoration alternatives. While we generally found correspondence between verbal and visual acceptability ratings across the entire sample, the groups distinguished by their verbal acceptability ratings did not substantially differ in how they rated the acceptability of the visual scenarios. Implications are discussed for designing and communicating the purpose and value of restoration treatments to stakeholder groups.
Biomass Assessment and Carbon Sequestration in Post-Fire Shrublands by Means of Sentinel-2 and Gaussian Processes
Authors: Vinué-Visús David , Ruiz-Peinado Ricardo , Fuente David , Oliver-Villanueva Jose-Vicente , Coll-Aliaga Eloína , Lerma-Arce Victoria
Published: May 2022

In this contribution, we assessed the biomass and carbon stock of a post-fire area covered by a young oak coppice of Quercus pyrenaica Willd. associated with shrubs, mainly of Cistus laurifolius L. This area was burned during the fire event of Chequilla (Guadalajara, Spain) in 2012. Sentinel-2 imagery was used together with our own forest inventories in 2020 and machine learning methods to assess the total biomass of the area. The inventory includes plots of total dry weight ranging between 6 and 14 Mg·ha−1 with individuals up to 8 years old. Nonlinear, nonparametric Gaussian process regression methods were applied to link reflectance values from Sentinel-2 imagery with total shrub biomass. With a reduced inventory of only 32 plots covering 136 ha, the total biomass could be assessed with a root-mean-square error of 1.36 Mg·ha−1 and a bias of −0.04 Mg·ha−1, getting a relative error between 9.8% and 20.4% for the gathered biomass. This is a rather good estimation considering the little effort and time invested; thus, the suggested methodology is very suitable for forest monitoring and management.
Effects of Nitrogen and Phosphorus Additions on Soil N2O Emissions and CH4 Uptake in a Phosphorus-Limited Subtropical Chinese Fir Plantation
Authors: Li Binjie , Chen Guangsheng , Lu Xiaochen , Jiao Hongbo
Published: May 2022

Increased nitrogen (N) inputs in subtropical forest ecosystems were widely reported. Extra N additions were reported to cause nutrient imbalance and phosphorus (P) limitation in many tropical and subtropical forests, and further result in changes in soil nitrous oxide (N2O) and methane (CH4) fluxes. Here, we conducted experiments with N (high N addition: 15 g N/m2, HN), P (low: 5 g P/m2, LP; high: 15 g P/m2, HP) and their interactive (HNLP and HNHP) treatments to investigate how N and P additions affected CH4 and N2O exchanges in an N-rich Chinese fir plantation (Cunninghamia lanceolata), and further explored the underlying mechanisms through the structural equation model (SEM) analysis. The results indicated that N addition alone (HN) significantly (p < 0.05) increased the soil N2O emissions by 30.15% and 80.47% over annual and 4-month periods, mainly owing to the elevated NH4+-N content. P addition alone (LP and HP) did not significantly affect the soil N2O emissions as compared with the control. The SEM analysis indicated that increased N2O emissions under N addition were primarily explained by the increase in available N and contributed more to the stimulated NH4+-N contents. N and P interactive additions slightly (not significant) stimulated the N2O emissions as compared with that under the N addition alone treatment. High-dose P addition significantly increased the soil CH4 uptake by 15.80% and 16.23% under the HP and HNHP treatments, respectively, while N addition alone and low P addition (LP and HNLP) did not significantly affect CH4 uptake as compared with the control. The increased water-soluble organic carbon and microbial biomass carbon explained the increased CH4 uptake under high P addition. The fertilization effects on N2O emissions and CH4 uptake mainly occurred in the first 4 months and diminished after that. Our results suggested that the direction, magnitude and timing of the N and P addition effects on N2O emissions and CH4 uptake would depend on the soil nutrient status and plant–microbial competition for N and P in subtropical forests.
Cofactor Self-Sufficient Whole-Cell Biocatalysts for the Relay-Race Synthesis of Shikimic Acid
Authors: Wang Xiaoshuang , Wu Fengli , Zhou Dan , Song Guotian , Chen Wujiu , Zhang Cuiying , Wang Qinhong
Published: May 2022

Shikimic acid (SA) is a key intermediate in the aromatic amino-acid biosynthetic pathway, as well as an important precursor for synthesizing many valuable antiviral drugs. The asymmetric reduction of 3-dehydroshikimic acid (DHS) to SA is catalyzed by shikimate dehydrogenase (AroE) using NADPH as the cofactor; however, the intracellular NADPH supply limits the biosynthetic capability of SA. Glucose dehydrogenase (GDH) is an efficient enzyme which is typically used for NAD(P)H regeneration in biocatalytic processes. In this study, a series of NADPH self-sufficient whole-cell biocatalysts were constructed, and the biocatalyst co-expressing Bmgdh–aroE showed the highest conversion rate for the reduction of DHS to SA. Then, the preparation of whole-cell biocatalysts by fed-batch fermentation without supplementing antibiotics was developed on the basis of the growth-coupled l-serine auxotroph. After optimizing the whole-cell biocatalytic conditions, a titer of 81.6 g/L SA was obtained from the supernatant of fermentative broth in 98.4% yield (mol/mol) from DHS with a productivity of 40.8 g/L/h, and cofactor NADP+ or NADPH was not exogenously supplemented during the whole biocatalytic process. The efficient relay-race synthesis of SA from glucose by coupling microbial fermentation with a biocatalytic process was finally achieved. This work provides an effective strategy for the biosynthesis of fine chemicals that are difficult to obtain through de novo biosynthesis from renewable feedstocks, as well as for biocatalytic studies that strictly rely on NAD(P)H regeneration.
Regulation of β-Disaccharide Accumulation by β-Glucosidase Inhibitors to Enhance Cellulase Production in Trichoderma reesei
Authors: Long Tingting , Zhang Peng , Yu Jingze , Gao Yushan , Ran Xiaoqin , Li Yonghao
Published: May 2022

Trichoderma reesei is a high-yield producer of cellulase for applications in lignocellulosic biomass conversion, but its cellulase production requires induction. A mixture of glucose and β-disaccharide has been demonstrated to achieve high-level cellulase production. However, as inducers, β-disaccharides are prone to be hydrolyzed by β-glucosidase (BGL) during fermentation, therefore β-disaccharides need to be supplemented through feeding to overcome this problem. Here, miglitol, an α-glucosidase inhibitor, was investigated as a BGL inhibitor, and exhibited an IC50 value of 2.93 μg/mL. The cellulase titer was more than two-fold when miglitol was added to the fermentation medium of T. reesei. This method was similar to the prokaryotic expression system using unmetabolized isopropyl-β-D-thiogalactopyranoside (IPTG) as the inducer instead of lactose to continuously induce gene expression. However, cellulase activity was not enhanced with BGL inhibition when lactose or cellulose was used as an inducer, which demonstrated that the transglycosidase activity of BGL is important for the inducible activity of lactose and cellulose. This novel method demonstrates potential in stimulating cellulase production and provides a promising system for T. reesei protein expression.
Evaluation of the Biocontrol Potential of a Commercial Yeast Starter against Fuel-Ethanol Fermentation Contaminants
Authors: Branco Patrícia , Diniz Mário , Albergaria Helena
Published: May 2022

Lactic acid bacteria (LAB) and Brettanomyces bruxellensis are the main contaminants of bioethanol fermentations. Those contaminations affect Saccharomyces cerevisiae performance and reduce ethanol yields and productivity, leading to important economic losses. Currently, chemical treatments such as acid washing and/or antibiotics are used to control those contaminants. However, these control measures carry environmental risks, and more environmentally friendly methods are required. Several S. cerevisiae wine strains were found to secrete antimicrobial peptides (AMPs) during alcoholic fermentation that are active against LAB and B. bruxellensis strains. Thus, in the present study, we investigated if the fuel-ethanol commercial starter S. cerevisiae Ethanol Red (ER) also secretes those AMPs and evaluated its biocontrol potential by performing alcoholic fermentations with mixed-cultures of ER and B. bruxellensis strains and growth assays of LAB in ER pre-fermented supernatants. Results showed that all B. bruxellensis strains were significantly inhibited by the presence of ER, although LAB strains were less sensitive to ER fermentation metabolites. Peptides secreted by ER during alcoholic fermentation were purified by gel-filtration chromatography, and a bioactive fraction was analyzed by ELISA and mass spectrometry. Results confirmed that ER secretes the AMPs previously identified. That bioactive fraction was used to determine minimal inhibitory concentrations (MICs) against several LAB and B. bruxellensis strains. MICs of 1–2 mg/mL were found for B. bruxellensis strains and above 2 mg/mL for LAB. Our study demonstrates that the AMPs secreted by ER can be used as a natural preservative in fuel-ethanol fermentations.
Substrate Selectivity of a Novel Amylo-α-1,6-glucosidase from Thermococcus gammatolerans STB12
Authors: Wang Yamei , Tian Yixiong , Ban Xiaofeng , Li Caiming , Hong Yan , Cheng Li , Gu Zhengbiao , Li Zhaofeng
Published: May 2022

Amylo-α-1,6-glucosidase (EC 3.2.1.33, AMY) exhibits hydrolytic activity towards α-1,6-glycosidic bonds of branched substrates. The debranching products of maltodextrin, waxy corn starch and cassava starch treated with AMY, pullulanase (EC 3.2.1.41, PUL) and isoamylase (EC 3.2.1.68, ISO), were investigated and their differences in substrate selectivity and debranching efficiency were compared. AMY had a preference for the branched structure with medium-length chains, and the optimal debranching length was DP 13–24. Its optimum debranching length was shorter than ISO (DP 25–36). In addition, the debranching rate of maltodextrin treated by AMY for 6 h was 80%, which was 20% higher than that of ISO. AMY could decompose most of the polymerized amylopectin in maltodextrin into short amylose and oligosaccharides, while it could only decompose the polymerized amylopectin in starch into branched glucan chains and long amylose. Furthermore, the successive use of AMY and β-amylase increased the hydrolysis rate of maltodextrin from 68% to 86%. Therefore, AMY with high substrate selectivity and a high catalytic capacity could be used synergistically with other enzyme preparations to improve substrate utilization and reduce reaction time. Importantly, the development of a novel AMY provides an effective choice to meet different production requirements.
HOG-SVM Impurity Detection Method for Chinese Liquor (Baijiu) Based on Adaptive GMM Fusion Frame Difference
Authors: Shi Xiaoshi , Tang Zuoliang , Wang Yihan , Xie Hong , Xu Lijia
Published: May 2022

Chinese liquor (Baijiu) is one of the four major distilled spirits in the world. At present, liquor products containing impurities still exist on the market, which not only damage corporate image but also endanger consumer health. Due to the production process and packaging technologies, impurities usually appear in products of Baijiu before entering the market, such as glass debris, mosquitoes, aluminium scraps, hair, and fibres. In this paper, a novel method for detecting impurities in bottled Baijiu is proposed. Firstly, the region of interest (ROI) is cropped by analysing the histogram projection of the original image to eliminate redundant information. Secondly, to adjust the number of distributions in the Gaussian mixture model (GMM) dynamically, multiple unmatched distributions are removed and distributions with similar means are merged in the process of modelling the GMM background. Then, to adaptively change the learning rates of the front and background pixels, the learning rate of the pixel model is created by combining the frame difference results of the sequence images. Finally, a histogram of oriented gradient (HOG) features of the moving targets is extracted, and the Support Vector Machine (SVM) model is chosen to exclude bubble interference. The experimental results show that this impurity detection method for bottled Baijiu controls the missed rate by within 1% and the false detection rate by around 3% of impurities. Its speed is five times faster than manual inspection and its repeatability index is good, indicating that the overall performance of the proposed method is better than manual inspection with a lamp. This method is not only efficient and fast, but also provides practical, theoretical, and technical support for impurity detection of bottled Baijiu that has broad application prospects.
Antioxidant Activity and Multi-Elemental Analysis of Dark Chocolate
Authors: Jaćimović Simona , Popović-Djordjević Jelena , Sarić Beka , Krstić Aleksandar , Mickovski-Stefanović Violeta , Pantelić Nebojša Đ.
Published: May 2022

Cocoa beans are part of the cocoa plant fruit (Theobroma cacao L.) used to prepare various products such as chocolate, cocoa butter, jelly, liqueurs, cosmetics, etc. Dark chocolate is consumed worldwide by different populations and is known for its good taste, making it one of the most favoured food products. This work aimed to determine the content of total polyphenols (TPC), total flavonoids (TFC), and the antioxidant potential measured through the ability to scavenge DPPH free radicals (DPPH), ferric reducing power (FRAP), and total antioxidant capacity (TAC), as well as major and trace elements contained in twelve commercially available dark chocolate samples, with cocoa content ranging from 40% to 99%. The total polyphenols content ranged between 10.55 and 39.82 mg/g GAE, while the total flavonoid content was from 10.04 to 37.85 mg/g CE. All applied antioxidant assays indicate that the sample with the highest cocoa percentage shows the greatest antioxidant activity (DPPH: 48.34% of inhibition; FRAP: 89.00 mg/g GAE; TAC: 83.86 mg/g AAE). Statistical methods were applied to establish the differences between the samples concerning TPC, TFC, DPPH, FRAP and TAC, as well as to differentiate the samples according to the mineral content. The results indicated that the differences in TPC and TFC between different samples depended on the cocoa content and the addition of dried fruit pieces. A good correlation between antioxidant potency composite index (ACI) and declared cocoa content was noticed (R2 = 0.8034), indicating that the declared percentage of cocoa is a reliable indicator for antioxidant activity of analysed dark chocolate samples. The nutritional evaluation proved that the studied chocolate samples were an excellent source of Mg, Fe, Mn and Cu.
Glucosinolates as Markers of the Origin and Harvesting Period for Discrimination of Bee Pollen by UPLC-MS/MS
Authors: Ares Ana M. , Tapia Jesús A. , González-Porto Amelia V. , Higes Mariano , Martín-Hernández Raquel , Bernal José
Published: May 2022

Bee pollen is currently one of the most commonly consumed food supplements, as it is considered to be a good source of bioactive substances and energy. It contains various health-promoting compounds, such as proteins, amino acids, lipids, as well as glucosinolates. In the present study, the glucosinolate content was determined, by means of ultra-performance liquid chromatography coupled to a quadrupole time-of-flight mass detector, in 72 bee pollen samples from four different apiaries in Guadalajara (Spain), harvested in three different periods. In addition, 11 commercial multifloral samples from different Spanish regions were also analyzed. The aim was to verify the suitability of these compounds as biomarkers of their geographical origin, and to test their potential for distinguishing the harvesting period. By means of a canonical discriminant analysis, it was possible to differentiate the apiary of origin of most of the samples, and these could also be clearly differentiated from the commercial ones, simply as a result of the glucosinolate content. In addition, it was also demonstrated for the first time that bee pollen samples were capable of being differentiated according to the time of harvesting and their glucosinolate content.
“Food Village”: An Innovative Alternative Food Network Based on Human Scale Development Economic Model
Authors: Stella Giordano , Torquati Biancamaria , Paffarini Chiara , Giordani Giorgia , Cecchini Lucio , Poletti Roberto
Published: May 2022

Although the different alternative food networks (AFNs) have experienced increases worldwide for the last thirty years, they are still unable to provide an alternative capable of spreading on a large scale. They in fact remain niche experiments due to some limitations on their structure and governance. Thus, this study proposes and applies a design method to build a new sustainable food supply chain model capable of realizing a “jumping scale”. Based on the theoretical and value framework of the Civil Economy (CE), the Economy for the Common Good (ECG), and the Development on a Human Scale (H-SD), the proposed design model aims to satisfy the needs of all stakeholders in the supply chain. Max-Neef’s Needs Matrix and Design Thinking (DT) tools were used to develop the design model. Applying the design method to the food chain has allowed us to develop the concept of the “Food Village”, an innovative food supply network far from the current economic mechanisms and based on the community and eco-sustainability.
Electrochemical Sensing of Vanillin Based on Fluorine-Doped Reduced Graphene Oxide Decorated with Gold Nanoparticles
Authors: Manikandan Venkatesh S. , Boateng Emmanuel , Durairaj Sharmila , Chen Aicheng
Published: May 2022

4-hydroxy-3-methoxybenzaldehyde (vanillin) is a biophenol compound that is relatively abundant in the world’s most popular flavoring ingredient, natural vanilla. As a powerful antioxidant chemical with beneficial antimicrobial properties, vanillin is not only used as a flavoring agent in food, beverages, perfumery, and pharmaceutical products, it may also be employed as a food-preserving agent, and to fight against yeast and molds. The widespread use of vanilla in major industries warrants the need to develop simple and cost-effective strategies for the quantitative determination of its major component, vanillin. Herein, we explore the applications of a selective and sensitive electrochemical sensor (Au electrodeposited on a fluorine-doped reduced-graphene-oxide-modified glassy-carbon electrode (Au/F-rGO/GCE)) for the detection of vanillin. The electrochemical performance and analytical capabilities of this novel electrochemical sensor were investigated using electrochemical techniques including cyclic voltammetry and differential pulse voltammetry. The excellent sensitivity, selectivity, and reproducibility of the proposed electrochemical sensor may be attributed to the high conductivity and surface area of the formed nanocomposite. The high performance of the sensor developed in the present study was further demonstrated with real-sample analysis.
Starch-Rich Microalgae as an Active Ingredient in Beer Brewing
Authors: Carnovale Giorgia , Leivers Shaun , Rosa Filipa , Norli Hans-Ragnar , Hortemo Edvard , Wicklund Trude , Horn Svein Jarle , Skjånes Kari
Published: May 2022

Microalgal biomass is widely studied for its possible application in food and human nutrition due to its multiple potential health benefits, and to address raising sustainability concerns. An interesting field whereby to further explore the application of microalgae is that of beer brewing, due to the capacity of some species to accumulate large amounts of starch under specific growth conditions. The marine species Tetraselmis chui is a well-known starch producer, and was selected in this study for the production of biomass to be explored as an active ingredient in beer brewing. Cultivation was performed under nitrogen deprivation in 250 L tubular photobioreactors, producing a biomass containing 50% starch. The properties of high-starch microalgal biomass in a traditional mashing process were then assessed to identify critical steps and challenges, test the efficiency of fermentable sugar release, and develop a protocol for small-scale brewing trials. Finally, T. chui was successfully integrated at a small scale into the brewing process as an active ingredient, producing microalgae-enriched beer containing up to 20% algal biomass. The addition of microalgae had a noticeable effect on the beer properties, resulting in a product with distinct sensory properties. Regulation of pH proved to be a key parameter in the process.
Lactiplantibacillus plantarum Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice
Authors: Zhang Lijuan , Meng Yuan , Li Jiayi , Yu Jiang , Mu Guangqing , Tuo Yanfeng
Published: May 2022

In our previous study, Lactiplantibacillus plantarum Y42 showed some potential probiotic functions and the ability to form biofilm. The aim of this study was to compare the similarities and differences in the probiotic and physiological traits of L. plantarum Y42 in the biofilm and planktonic states. L. plantarum Y42 in the biofilm state was proven to have higher survival after passing through mimic gastrointestinal fluid, as well as excellent adhesion properties on the HT-29 cell monolayers, than those in the planktonic state. The expression of tight junction proteins (TJ proteins) of HT-29 cell monolayers treated by L. plantarum Y42 in the planktonic state increased, while similar changes were not observed in the HT-29 cells treated by the strain in the biofilm state. Furthermore, Balb/c mice were orally administered L. plantarum Y42 in the biofilm and planktonic states, respectively. Compared to the planktonic state, the oral administration of L. plantarum Y42 in the biofilm state significantly boosted IgA levels and improved the immunity of the mice. High-throughput sequencing showed that the diversity and structure of the intestinal flora of the mice were changed after the oral administration of L. plantarum Y42, including the up-regulated relative abundance of Lactobacillus in the intestinal tract of the mice, with no difference between the biofilm and planktonic states. Moreover, oral administration of L. plantarum Y42 in biofilm and planktonic states reduced the release of proinflammatory factors, to a certain extent, in the serum of the mice. The similarities and differences in the probiotic and physiological properties of L. plantarum Y42 in the biofilm and planktonic states can be contributed to the reasonable application of the strain.
Application of a Rapid and Simple Technological Process to Increase Levels and Bioccessibility of Free Phenolic Compounds in Annurca Apple Nutraceutical Product
Authors: Maisto Maria , Schiano Elisabetta , Novellino Ettore , Piccolo Vincenzo , Iannuzzo Fortuna , Salviati Emanuela , Summa Vincenzo , Annunziata Giuseppe , Tenore Gian Carlo
Published: May 2022

Insoluble bound polyphenols (ISBP) are polyphenolic compounds linked to the food matrix with different interactions limiting both their water extractability and consequent bioaccessibility. The health-promoting potential of polyphenols is historically known and well-demonstrated; specifically, Annurca apple polyphenols were studied both in vitro and in vivo for their effect in controlling cholesterol plasma levels. The aim of the study was the preparation of nutraceutical products based on Annurca apple polyphenolic fraction through the application of a technological process (acid treatment) able to release the ISBP from Annurca apple food matrix and increase polyphenol bioaccessibility. Lyophilized annurca apple (LAA) underwent acid treatment (ATLAA), and differences in released polyphenol levels were analysed by DAD-HPLC. Free-polyphenol levels in samples treated under acid conditions were higher than in untreated ones; in particular, for oligomeric flavan-3-ols (+168% procyanidin B2, +42.97% procyanidin B1 and B2, +156.99% procyanidin C1), catechin (+512.20%), and gallic acid (+707.77%). Furthermore, ATLAA underwent an in vitro gastrointestinal digestion to evaluate the bioaccessibility of contained polyphenols, in comparison to the untreated Annurca apple. The bioaccessibility study indicates a valuable preservation of polyphenolic fraction compared to the control.
New Insights into the Neuromyogenic Spectrum of a Gain of Function Mutation in SPTLC1
Authors: Kölbel Heike , Kraft Florian , Hentschel Andreas , Czech Artur , Gangfuss Andrea , Mohassel Payam , Nguyen Chi , Stenzel Werner , Schara-Schmidt Ulrike , Preuße Corinna , Roos Andreas
Published: May 2022

Serine palmitoyltransferase long chain base subunit 1 (SPTLC1) encodes a serine palmitoyltransferase (SPT) resident in the endoplasmic reticulum (ER). Pathological SPTLC1 variants cause a form of hereditary sensory and autonomic neuropathy (HSAN1A), and have recently been linked to unrestrained sphingoid base synthesis, causing a monogenic form of amyotrophic lateral sclerosis (ALS). It was postulated that the phenotypes associated with dominant variants in SPTLC1 may represent a continuum between neuropathy and ALS in some cases, complicated by additional symptoms such as cognitive impairment. A biochemical explanation for this clinical observation does not exist. By performing proteomic profiling on immortalized lymphoblastoid cells derived from one patient harbouring an alanine to serine amino acid substitution at position 20, we identified a subset of dysregulated proteins playing significant roles in neuronal homeostasis and might have a potential impact on the manifestation of symptoms. Notably, the identified p.(A20S)-SPTLC1 variant is associated with decrease of transcript and protein level. Moreover, we describe associated muscle pathology findings, including signs of mild inflammation accompanied by dysregulation of respective markers on both the protein and transcript levels. By performing coherent anti-Stokes Raman scattering microscopy, presence of protein and lipid aggregates could be excluded.
Interleukin 6 SNP rs1818879 Regulates Radiological and Inflammatory Activity in Multiple Sclerosis
Authors: Bruno Antonio , Dolcetti Ettore , Azzolini Federica , Moscatelli Alessandro , Gambardella Stefano , Ferese Rosangela , Rizzo Francesca Romana , Gilio Luana , Iezzi Ennio , Galifi Giovanni , Borrelli Angela , Buttari Fabio , Furlan Roberto , Finardi Annamaria , De Vito Francesca , Musella Alessandra , Guadalupi Livia , Mandolesi Georgia , Centonze Diego , Stampanoni Bassi Mario
Published: May 2022

(1) Background: The clinical course of multiple sclerosis (MS) is critically influenced by the expression of different pro-inflammatory and anti-inflammatory cytokines. Interleukin 6 (IL-6) represents a major inflammatory molecule previously associated with exacerbated disease activity in relapsing remitting MS (RR-MS); however, the role of single-nucleotide polymorphisms (SNPs) in the IL-6 gene has not been fully elucidated in MS. (2) Methods: We explored in a cohort of 171 RR-MS patients, at the time of diagnosis, the associations between four IL-6 SNPs (rs1818879, rs1554606, rs1800797, and rs1474347), CSF inflammation, and clinical presentation. (3) Results: Using principal component analysis and logistic regression analysis we identified an association between rs1818879, radiological activity, and a set of cytokines, including the IL-1β, IL-9, IL-10, and IL-13. No significant associations were found between other SNPs and clinical or inflammatory parameters. (4) Conclusions: The association between the rs1818879 polymorphism and subclinical neuroinflammatory activity suggests that interindividual differences in the IL-6 gene might influence the immune activation profile in MS.
Pectin-Based Edible Coating Combined with Chemical Dips Containing Antimicrobials and Antibrowning Agents to Maintain Quality of Fresh-Cut Pears
Authors: Pleșoianu Alina Mădălina , Nour Violeta
Published: May 2022

The aim of this study was to assess the effects of pectin coating alone (PE) or combined with chemical dips containing potassium sorbate (PS) or sodium benzoate (SB) as antimicrobials, and N-acetyl cysteine (N-AC) or ascorbic acid (AA) + citric acid (CA) as antibrowning agents, on weight loss, color values, browning index, firmness, titratable acidity, soluble solids content, total phenolic content, antioxidant activity and sensory attributes of fresh-cut pears during 15-day storage at 8 °C. Pectin coating delayed weight loss and improved firmness of fresh-cut pears as compared to control samples. Addition of either 1% N-AC or 1% CA + 1% AA in the formulation of the chemical dip protected the phenolic compounds and enhanced the antioxidant activity of fresh-cut pears during storage. PE + 0.2% SB + 1% N-AC and PE + 0.2% PS + 1% N-AC were the most efficient treatments in preserving color and reducing the browning index of fresh-cut pears during 15-day storage at 8 °C and received the highest scores for all sensory attributes throughout 12 days of storage. The results demonstrate the feasibility of PE + 0.2% SB + 1% N-AC and PE + 0.2% PS + 1% N-AC for extending the shelf life of fresh-cut pears.
The Mediating Role of Dispositional Optimism in the Relationship between Health Locus of Control and Self-Efficacy in Pregnant Women at Risk of Preterm Delivery
Authors: Niewiadomska Iwona , Bień Agnieszka , Rzońca Ewa , Jurek Krzysztof
Published: May 2022

Difficult situations during pregnancy, such as threatened preterm labor, trigger negative experiences in women. The levels of stress experienced and the way individuals cope with it depend on their personal resources, such as optimism, internal health locus of control, and self-efficacy, among other factors. The purpose of this paper was to determine the role of dispositional optimism in the relationship between health locus of control and self-efficacy in pregnant women with threatened preterm labor. Dispositional optimism plays the role of mediator in relationships between: (1) internal health control and self-efficacy; and (2) impact of random events on one’s health and self-efficacy. Dispositional optimism does not mediate the relationship between the perceived impact of others on one’s health and self-efficacy. For women with a high-risk pregnancy, dispositional optimism is a significant resource for coping with the problems they encounter. It changes the direction (from negative to positive) of the association between experiencing the impact of external factors (random events) on one’s health and perceived self-efficacy. It prevents the cycle of loss caused by the interpretation of random events as having an impact on one’s health, and acts as a mediator to initiate a cycle of gains that leads to greater perceived self-efficacy. Optimistic pregnant women maintain a positive outlook, even when confronted with difficult, negative experiences such as threatened preterm labor.
Blood Lead Monitoring in a Former Mining Area in Euskirchen, Germany—Volunteers across the Entire Population
Authors: Bertram Jens , Ramolla Christian , Esser André , Schettgen Thomas , Fohn Nina , Kraus Thomas
Published: May 2022

After centuries of mining in the district of Euskirchen, that is, in the communities of Mechernich and Kall, the lead concentration in the soil remains high, often exceeding regulatory guidelines. To clarify the lead body burden among residents in the region, a human biomonitoring study on a voluntary basis was initiated in which the blood lead level (BLL) was assessed. A questionnaire was distributed to evaluate lead exposure routes and confounders. Overall, 506 volunteers participated in the study, of whom 7.5% were children and adolescents, 71.9% were adults from 18 to 69 years, and 19.4% were residents 70 years or older. While the BLLs in the adult population were inconspicuous, among the children and adolescents investigated, 16.7% of the children between 3 and 17 years had BLLs above the recently revised German reference values for BLL in children. These results point towards a higher lead exposure in children living in the region. The hierarchical regression analysis based on the BLL and the questionnaire revealed the significant influence of the factors age, sex, smoking, construction age of the real estate, occupancy, and intensive contact with soil on the BLL. Measures to reduce lead exposure include a focus on improved personal and domestic hygiene to minimize lead intake.
Trauma-Directed Interaction (TDI): An Adaptation to Parent-Child Interaction Therapy for Families with a History of Trauma
Authors: Gurwitch Robin H. , Warner-Metzger Christina M.
Published: May 2022

Parent-Child Interaction Therapy (PCIT) is one of the strongest evidence-based treatments available for young children and their families. Research has supported the use of PCIT for children with a history of trauma; however, the treatment does not directly address trauma in the child. PCIT is a dyadic treatment; yet, the impact of the carer’s trauma on the carer-child relationship is not assessed or incorporated into treatment. For these reasons, therapists, families, agencies, and funders tend to view PCIT as a trauma treatment with skepticism. PCIT therapists who currently address trauma within the intervention do so without a standardized approach. Trauma-Directed Interaction (TDI) is an adaptation developed to directly address these concerns. TDI maintains the key elements and theoretical underpinnings of PCIT while adding sessions to cover psychoeducation about trauma, carer response to a child’s trauma reactions (SAFE skills), and coping skills to aid both the child and the carer to manage trauma activators (COPE skills). The TDI module creates a consistent strategy for PCIT therapists to address trauma, thus allowing research and replication which will advance the dual fields of PCIT and family trauma. The theoretical conceptualization of TDI is presented along with next steps in its evaluation.
Adipocytokines and Insulin Resistance: Their Role as Benign Breast Disease and Breast Cancer Risk Factors in a High-Prevalence Overweight-Obesity Group of Women over 40 Years Old
Authors: Sat-Muñoz Daniel , Martínez-Herrera Brenda-Eugenia , Quiroga-Morales Luis-Aarón , Trujillo-Hernández Benjamín , González-Rodríguez Javier-Andrés , Gutiérrez-Rodríguez Leonardo-Xicotencatl , Leal-Cortés Caridad-Aurea , Portilla-de-Buen Eliseo , Rubio-Jurado Benjamín , Salazar-Páramo Mario , Gómez-Sánchez Eduardo , Delgadillo-Cristerna Raúl , Carrillo-Nuñez Gabriela-Guadalupe , Nava-Zavala Arnulfo-Hernán , Balderas-Peña Luz-Ma-Adriana
Published: May 2022

Insulin levels, adipocytokines, and inflammatory mediators trigger benign breast disease (BBD) and breast cancer (BC). The relationship between serum adipocytokines levels, overweight-obesity, metabolic disturbs, and BC is unclear. Methods: To analyze the serum levels of the adipocytokines, insulin, and the HOMA IR in women without breast disease, with BBD or BC, and the role of these as risk factors for benign breast disease or breast cancer. Results: Adipsin values > 0.91 and visfatin levels > 1.18 ng/mL represent a risk factor to develop BBD in NBD lean women (OR = 18; and OR = 12). Data in overweight-obese women groups confirm the observation due to insulin levels > 2.6 mU/mL and HOMA IR > 0.78, with OR = 60.2 and 18, respectively; adipsin OR = 26.4, visfatin OR = 12. Breast cancer risk showed a similar behavior: Adipsin risk, adjusted by insulin and visfatin OR = 56 or HOMA IR and visfatin OR = 22.7. Conclusion: Adipose tissue is crucial for premalignant and malignant tissue transformation in women with overweight-obesity. The adipocyte–breast epithelium interaction could trigger a malignant transformation in a continuum, starting with BBD as premalignant disease, especially in overweight-obese women.
Factors Associated with Screening Mammogram Uptake among Women Attending an Urban University Primary Care Clinic in Malaysia
Authors: Abdullah Nasturah , Baharudin Noorhida , Mohamad Mariam , Mohamed-Yassin Mohamed-Syarif
Published: May 2022

Screening mammograms have resulted in a reduction in breast cancer mortality, yet the uptake in Malaysia was low. This study aimed to determine the prevalence and factors associated with screening mammogram uptake among women attending a Malaysian primary care clinic. A cross-sectional study was conducted among 200 women aged 40 to 74 attending the clinic. The data was collected using questionnaires assessing sociodemographic, clinical characteristics, knowledge and health beliefs. Multiple logistic regression was used to identify factors associated with mammogram uptake. The prevalence of screening mammograms was 46.0%. About 45.5% of women with high breast cancer risk had never undergone a mammogram. Older participants, aged 50 to 74 (OR = 2.57, 95% CI: 1.05, 6.29, p-value = 0.039) and those who received a physician’s recommendation (OR = 7.61, 95% CI: 3.81, 15.20, p-value < 0.001) were more likely to undergo screening mammography. Significant health beliefs associated with mammogram uptake were perceived barriers (OR = 0.81, 95% CI: 0.67, 0.97, p-value = 0.019) and cues to action (OR = 1.30, 95% CI: 1.06, 1.59, p-value = 0.012). Approximately half of the participants and those in the high-risk group had never undergone a mammogram. Older age, physician recommendation, perceived barriers and cues to action were significantly associated with mammogram uptake. Physicians need to play an active role in promoting breast cancer screening and addressing the barriers.
Role of Biliverdin Reductase A in the Regulation of Insulin Signaling in Metabolic and Neurodegenerative Diseases: An Update
Authors: Cimini Flavia Agata , Perluigi Marzia , Barchetta Ilaria , Cavallo Maria Gisella , Barone Eugenio
Published: May 2022

Insulin signaling is a conserved pathway that orchestrates glucose and lipid metabolism, energy balance, and inflammation, and its dysregulation compromises the homeostasis of multiple systems. Insulin resistance is a shared hallmark of several metabolic diseases, including obesity, metabolic syndrome, and type 2 diabetes, and has been associated with cognitive decline during aging and dementia. Numerous mechanisms promoting the development of peripheral and central insulin resistance have been described, although most of them were not completely clarified. In the last decades, several studies have highlighted that biliverdin reductase-A (BVR-A), over its canonical role in the degradation of heme, acts as a regulator of insulin signaling. Evidence from human and animal studies show that BVR-A alterations are associated with the aberrant activation of insulin signaling, metabolic syndrome, liver steatosis, and visceral adipose tissue inflammation in obese and diabetic individuals. In addition, recent findings demonstrated that reduced BVR-A levels or impaired BVR-A activation contribute to the development of brain insulin resistance and metabolic alterations in Alzheimer’s disease. In this narrative review, we will provide an overview on the literature by focusing on the role of BVR-A in the regulation of insulin signaling and how BVR-A alterations impact on cell dysfunctions in both metabolic and neurodegenerative disorders.
Regulation of Translation, Translocation, and Degradation of Proteins at the Membrane of the Endoplasmic Reticulum
Authors: Daverkausen-Fischer Lea , Draga Margarethe , Pröls Felicitas
Published: May 2022

The endoplasmic reticulum (ER) of mammalian cells is the central organelle for the maturation and folding of transmembrane proteins and for proteins destined to be secreted into the extracellular space. The proper folding of target proteins is achieved and supervised by a complex endogenous chaperone machinery. BiP, a member of the Hsp70 protein family, is the central chaperone in the ER. The chaperoning activity of BiP is assisted by ER-resident DnaJ (ERdj) proteins due to their ability to stimulate the low, intrinsic ATPase activity of BiP. Besides their co-chaperoning activity, ERdj proteins also regulate and tightly control the translation, translocation, and degradation of proteins. Disturbances in the luminal homeostasis result in the accumulation of unfolded proteins, thereby eliciting a stress response, the so-called unfolded protein response (UPR). Accumulated proteins are either deleterious due to the functional loss of the respective protein and/or due to their deposition as intra- or extracellular protein aggregates. A variety of metabolic diseases are known to date, which are associated with the dysfunction of components of the chaperone machinery. In this review, we will delineate the impact of ERdj proteins in controlling protein synthesis and translocation under physiological and under stress conditions. A second aspect of this review is dedicated to the role of ERdj proteins in the ER-associated degradation pathway, by which unfolded or misfolded proteins are discharged from the ER. We will refer to some of the most prominent diseases known to be based on the dysfunction of ERdj proteins.
The PagKNAT2/6b-PagBOP1/2a Regulatory Module Controls Leaf Morphogenesis in Populus
Authors: Zhao Yanqiu , Zhang Yifan , Zhang Weilin , Shi Yangxin , Jiang Cheng , Song Xueqin , Tuskan Gerald A. , Zeng Wei , Zhang Jin , Lu Mengzhu
Published: May 2022

Leaf morphogenesis requires precise regulation of gene expression to achieve organ separation and flat-leaf form. The poplar KNOTTED-like homeobox gene PagKNAT2/6b could change plant architecture, especially leaf shape, in response to drought stress. However, its regulatory mechanism in leaf development remains unclear. In this work, gene expression analyses of PagKNAT2/6b suggested that PagKNAT2/6b was highly expressed during leaf development. Moreover, the leaf shape changes along the adaxial-abaxial, medial-lateral, and proximal-distal axes caused by the mis-expression of PagKNAT2/6b demonstrated that its overexpression (PagKNAT2/6b OE) and SRDX dominant repression (PagKNAT2/6b SRDX) poplars had an impact on the leaf axial development. The crinkle leaf of PagKNAT2/6b OE was consistent with the differential expression gene PagBOP1/2a (BLADE-ON-PETIOLE), which was the critical gene for regulating leaf development. Further study showed that PagBOP1/2a was directly activated by PagKNAT2/6b through a novel cis-acting element “CTCTT”. Together, the PagKNAT2/6b-PagBOP1/2a module regulates poplar leaf morphology by affecting axial development, which provides insights aimed at leaf shape modification for further improving the drought tolerance of woody plants.
Genetic Elements Orchestrating Lactobacillus crispatus Glycogen Metabolism in the Vagina
Authors: Hertzberger Rosanne , May Ali , Kramer Gertjan , van Vondelen Isabelle , Molenaar Douwe , Kort Remco
Published: May 2022

Glycogen in the female lower reproductive tract is a major carbon source for colonization and acidification by common vaginal Lactobacillus species, such as Lactobacillus crispatus. Previously, we identified the amylopullulanase encoding gene pulA of Lactobacillus crispatus to correlate with the ability to autonomously utilize glycogen for growth. Here, we further characterize genetic variation and differential regulation of pulA affecting the presence of its gene product on the outer surface layer. We show that alpha-glucan degrading activity dissipates when Lactobacillus crispatus is grown on glucose, maltose and maltotriose, in agreement with carbon catabolite repression elements flanking the pulA gene. Proteome analysis of the S-layer confirmed that the amylopullulanase protein is highly abundant in an S-layer enriched fraction, but not in a strain with a defective amylopullulanase variant or in an amylopullulanase-sufficient strain grown on glucose. In addition, we provide evidence that Lactobacillus crispatus pulA mutants are relevant in vivo, as they are commonly observed in metagenome datasets of human vaginal microbial communities. Analysis of the largest publicly available dataset of 1507 human vaginal metagenomes indicates that among the 270 samples that contain a Lactobacillus crispatuspulA gene, 62 samples (23%) had a defective variant of this gene. Taken together, these results demonstrate that both environmental, as well as genetic factors explain the variation of Lactobacillus crispatus alpha-glucosidases in the vaginal environment.