bluuebunny/crossref_metadata_2025 huggingface dataset:
Artificial Intelligence Models for Predicting Ground Vibrations in Deep Underground Mines to Ensure the Safety of Their Surroundings
Authors: Tao Yunbo , Chen Qiusong , Xiao Chongchun , Zhu Min , Qiu Jianhui
Published: May 2024

Ground vibrations induced by underground mining blasting has a significant impact on the stability and safety of surface buildings near mines. Due to the thick rock layers overlying underground mines, there is presently limited accuracy in regard to predicting ground vibrations induced by underground mine blasting. Therefore, this study aims to improve the accuracy of predicting ground vibrations induced by underground blasting by comprehensively measuring the peak particle velocity (PPV) in all three directions and independently considering on the impact of vertical distance. Random forest regression (RFR), bagging regression (BR), and gradient boosting regression (GBR) were used to regress the X-axis PPV (X-PPV), Y-axis PPV (Y-PPV), and Z-axis PPV (Z-PPV) based on blasting records measured at an iron mine. In addition, a genetic algorithm, gray wolf optimizer (GWO), and a particle swarm optimization were used to optimize the parameters of the RFR, BR, and GBR. The comparison results show that GWO-GBR is the optimal model for the prediction of the X-PPV (R2 = 0.8072), Y-PPV (R2 = 0.9147), and Z-PPV (R2 = 0.9265), respectively. Thus, the GWO-GBR model proposed in this study is considered a highly reliable model for predicting ground vibrations induced by underground mine blasting to ensure the safety of the mines’ surroundings.
Virtual Reality-Powered Wrist Therapy: Developing a Therapist-Driven Exit-the-Room Serious Game with Hand Gesture Interactions
Authors: Baranyi René , Hirber Christoph , Roehrling Lukas , Aigner Christoph , Hoelbling Dominik , Hoerner Werner , Grechenig Thomas
Published: May 2024

Wrist injuries, driven by factors such as an aging population and the popularity of high-impact sports, have become increasingly prevalent. In response, this study focuses on developing a serious game for wrist injury rehabilitation within a virtual environment, aiming to enhance motivation and therapeutic adherence while highlighting the potential of virtual rehabilitation. Extensive literature research and the involvement of five experts during the game’s design led to the creation of “WristBreakout”, which successfully integrated 13 out of 15 recommended movements into gameplay. In addition, the technical feasibility of the VR headset “MetaQuest” within the domain of wrist rehabilitation was shown. A preliminary evaluation with six healthy participants showed positive results in terms of usability and acceptance (SUS average was 69; UES categories were between 3.97 and 4.77). This work contributes to the broader context of serious games and virtual reality (VR) applications in healthcare, exemplifying how technology can positively impact the rehabilitation experience.
The Fault Diagnosis of a Plunger Pump Based on the SMOTE + Tomek Link and Dual-Channel Feature Fusion
Authors: Yang Xiwang , Xu Xiaoyan , Wang Yarong , Liu Siyuan , Bai Xiong , Jing Licheng , Ma Jiancheng , Huang Jinying
Published: May 2024

Mechanical condition monitoring data in real engineering are often severely unbalanced, which can lead to a decrease in the stability and accuracy of intelligent diagnosis methods. In this paper, a fault diagnosis method based on the SMOTE + Tomek Link and dual-channel feature fusion is proposed to improve the performance of the sample imbalance fault diagnosis method, taking the piston pump of a turnout rutting machine as the research object. Combining the data undersampling method and the oversampling method to redistribute the collected normal data and fault data makes the diagnostic model have better diagnostic performance in the case of insufficient fault samples. And, in order to fully utilize the global features and local features, a global–local feature complementary module (GLFC) is proposed. Firstly, the generated data similar to the original data are constructed using the SMOTE + Tomek Link method; secondly, the generated data are input into a GLFC module and BiGRU at the same time, the GLFC module extracts the spatial global features and local features of the original vibration data, and BiGRU extracts the temporal information features of the original vibration data, and fuses the extracted feature information, and inputs the fused features into the attention layer; finally, a GLFC module is proposed by the SMOTE + Tomek Link method to make full use of the global features and local features. The extracted feature information is fused, and the fused features are input to the attention layer; finally, the fault classification is completed by the softmax classifier. In this paper, the accuracy and robustness of the proposed model are demonstrated through experiments.
The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions
Authors: Dyduch-Siemińska Magdalena , Gawroński Jacek
Published: May 2024

The efficiency and method of regeneration in in vitro culture conditions depend primarily on the plant growth regulators (PGRs) used. Even growth regulators belonging to one group may have different effects, stimulating the process of direct or indirect organogenesis, thus possibly disturbing the genetic stability among regenerants. The main aim of this study was to identify the genetic stability of Scutellaria baicalensis regenerates obtained by in vitro culture method using start codon targeted (ScoT) markers. S. baicalensis nodal explants were regenerated on MS medium supplemented with kinetin (KIN) at concentrations of 0.25, 0.5, 1.0, and 2.0 mg × dm−3 or benzylaminopurine (BAP)—0.25, 0.5, 1.0, and 2.0 mg × dm−3. The effects of the number of propagated shoots, length, number of nodes, and fresh mass of regenerants were assessed. Moreover, the genetic stability of the regenerants was analyzed using start codon targeted (SCoT) markers. Direct shoot organogenesis was observed on an MS medium containing kinetin, while indirect shoot induction occurred on an MS medium supplemented with BAP. The highest average number of shoots (3.6) was achieved for the MS + KIN medium at a concentration of 0.25 and 5.8 for the MS + BAP 1.0 medium. The average length and average number of nodes were the highest on the MS + BAP 0.25 medium (50.0 and 6.0, respectively), while the lowest values of these features were observed on the MS + KIN 2.0 medium (40.3 and 4.9, respectively). A total of 111 amplified bands were exhibited by SCoT primers. Three of the analyzed primers revealed four unique genotype-specific markers. The average percentage of polymorphism obtained was 36.7%. The analysis of genetic similarity revealed a high level of genetic similarity between the donor plant and regenerants obtained on MS “0” (medium without the addition of phytohormones). A slightly lower value of genetic similarity was observed for regenerants obtained by direct organogenesis (MS + KIN medium at all concentrations). Indirect shoot organogenesis observed on the MS + BAP medium (all concentrations) resulted in the highest differentiation, both in relation to the donor plant and MS “0” regenerants. The results of our work indicate that, in the case of S. baicalensis, the maintenance of genetic stability depends primarily on the presence of the cytokinin type in the medium.
Long-Term Changes in the Permafrost Temperature and Surface Frost Number in Northeast China
Authors: Shan Wei , Qiu Lisha , Guo Ying , Zhang Chengcheng , Liu Shuai
Published: May 2024

The permafrost in Northeast China is experiencing rapid degradation due to the influence of climate change and human activities, profoundly impacting the local ecological environment and engineering construction. Understanding the spatiotemporal dynamics of long-term permafrost in this region is crucial; however, systematic research on this topic remains scarce. This study combines meteorological station data, MODIS land surface temperature (LST) datasets, and borehole locations to apply the surface frost number (SFn) model. This approach enables the simulation and estimation of the spatial distribution and changes in the area of the surface frost number without vegetation effects (SFnv) and permafrost temperature (PT) in Northeast China from 1971 to 2020. The area of the SFnv > 0.49 within the permafrost region decreased substantially from approximately 44.353 × 104 km2 to 19.909 × 104 km2 between 1971 and 2020, with a notable change in 1988. The area of permafrost calculated using PT < 0 was slightly smaller, declining from 39.388 × 104 km2 to 29.852 × 104 km2. There was also a significant increase in the area with PT ranging from −1 °C to 0 °C, indicating a decline in permafrost stability. Approximately 10.926 × 104 km2 of stable permafrost has been transformed into semi-stable and unstable permafrost. Moreover, from 1982 to 2020, the NDVI was negatively correlated with the area of stable permafrost and positively correlated with the area of transitional or unstable permafrost. Vegetation cover decreased as transitional or unstable permafrost degraded. These findings provide valuable information for permafrost research and engineering development in cold regions, as well as for future planning and adaptation strategies.
Accelerating the Low-Carbon Energy Transition in Sub-Saharan Africa through Floating Photovoltaic Solar Farms
Authors: Ingo Tarelayefa Igedibor , Gyoh Louis , Sheng Yong , Kaymak Mustafa Kemal , Şahin Ahmet Duran , Pouran Hamid M.
Published: May 2024

Climate change has become a global issue and is predicted to impact less-developed regions, such as sub-Saharan Africa, severely. Innovative, sustainable renewable energy systems are essential to mitigate climate change’s effects and unlock the region’s potential, especially with the increasing energy demands and population growth. The region relies heavily on fossil fuels, which calls for urgent action towards energy security and expansion. Hybrid floating solar photovoltaic-hydropower (FPV-HEP) technology has emerged as a cost-effective and transformative solution to accelerate the low-carbon energy transition in sub-Saharan Africa. The technology combines solar panels with existing hydropower infrastructure, ensuring energy security while reducing carbon emissions. This technology offers several benefits over conventional ground-mounted solar systems, including efficient land utilization, energy generation, and water conservation. However, its adoption remains challenging due to technical complexities and evolving regulatory frameworks. Despite these challenges, Nigerian energy professionals have preferred renewable alternatives, mainly distributed solar PV and FPV-HEP plants. This collective embrace of FPV and renewables reflects a growing understanding of their critical role in mitigating climate change through sustainable energy practices. This research aims to contribute to the existing body of knowledge and assist policymakers in making informed decisions on adopting this technology. It also stimulates further research on this topic, offering a new potential solution to the ever-increasing demand for green energy in the region to meet their sustainable development needs.
Seasonal Cycle of Southern Hemisphere Explosive Growth and Decay of Storms
Authors: Osbrough Stacey L. , Frederiksen Jorgen S.
Published: May 2024

The seasonal variability of Southern Hemisphere (SH) synoptic-scale weather systems is analysed for the 20-year timespan 1997 to 2016. The relationships between the SH jet streams and storm tracks based on lower tropospheric circulation anomalies filtered into the high-pass (periods < 4 days) and band-pass (periods between 4 and 8 days) types are examined based on 6-hourly reanalysis data. Leading Empirical Orthogonal Functions (EOFs) and storm tracks based on all (growing and decaying) disturbances are determined. As well, the structure and standard deviations of streamfunction fluctuations are determined separately in three growth rate and three decay rate bins focusing on explosive growth and decay. In all cases, and in each season, the band-pass storm tracks are more zonally symmetric than the high-pass standard deviations and this is also reflected by the EOFs. Leading EOFs in both bands are monopole wavetrains of highs and lows located in the storm tracks with some band-pass disturbances having dipole structures consistent with blocking and northwest cloud bands. EOFs based on the bin with slow-growing fluctuations are structurally similar to the standard EOFs based on all disturbances. EOFs for moderately and explosively growing disturbances are increasingly displaced equatorward with a larger growth rate.
Assessment of Satellite Products in Estimating Tropical Cyclone Remote Precipitation over the Yangtze River Delta Region
Authors: Wu Xinyue , Liu Yebing , Liu Shulan , Jin Yubing , Xu Huiyan
Published: May 2024

Satellite products have shown great potential in estimating torrential rainfall due to their wide and consistent global coverage. This study assessed the monitoring capabilities of satellite products for the tropical cyclone remote precipitation (TRP) over the Yangtze River Delta region (YRDR) associated with severe typhoon Khanun (2017) and super-typhoon Mangkhut (2018). The satellite products include the CPC MORPHing technique (CMORPH) data, Tropical Rainfall Measuring Mission 3B42 Version 7 (TRMM 3B42), and Integrated Multi-satellite Retrievals for the Global Precipitation Measurement Mission (GPM IMERG). Eight precision evaluation indexes and statistical methods were used to analyze and evaluate the monitoring capabilities of CMORPH, TRMM 3B42, and GPM IMERG satellite precipitation products. The results indicated that the monitoring capability of TRMM satellite precipitation products was superior in capturing the spatial distribution, and GPM products captured the temporal distributions and different category precipitation observed from gauge stations. In contrast, the CMORPH products performed moderately during two heavy rainfall events, often underestimating or overestimating precipitation amounts and inaccurately detecting precipitation peaks. Overall, the three satellite precipitation products showed low POD, high FAR, low TS, and high FBIAS for heavy rainfall events, and the differences in monitoring torrential TRP may be related to satellite retrieval algorithms.
On the Use of Randomly Selected Partial Charges to Predict Battery State-of-Health
Authors: Vilsen Søren B. , Stroe Daniel-Ioan
Published: May 2024

As society becomes more reliant on Lithium-ion (Li-ion) batteries, state-of-health (SOH) estimation will need to become more accurate and reliable. Therefore, SOH modelling is in the process of shifting from using simple and continuous charge/discharge profiles to more dynamic profiles constructed to mimic real operation when ageing the Li-ion batteries. However, in most cases, when ageing the batteries, the same exact profile is just repeated until the battery reaches its end of life. Using data from batteries aged in this fashion to create a model, there is a very real possibility that the model will rely on the built-in repetitiveness of the profile. Therefore, this work will examine the dependence of the performance of a multiple linear regression on the number of charges used to train the model, and their location within the profile used to age the batteries. The investigation shows that it is possible to train models using randomly selected partial charges while still reaching errors as low as 0.5%. Furthermore, it shows that only one randomly sampled partial charge is needed to achieve errors smaller than 1%. Lastly, as the number of randomly sampled partial charges used to train the model increases, the dependence on particular partial charges tends to decrease.
Explainable Precision Medicine in Breast MRI: A Combined Radiomics and Deep Learning Approach for the Classification of Contrast Agent Uptake
Authors: Nowakowska Sylwia , Borkowski Karol , Ruppert Carlotta , Hejduk Patryk , Ciritsis Alexander , Landsmann Anna , Marcon Magda , Berger Nicole , Boss Andreas , Rossi Cristina
Published: May 2024

In DCE-MRI, the degree of contrast uptake in normal fibroglandular tissue, i.e., background parenchymal enhancement (BPE), is a crucial biomarker linked to breast cancer risk and treatment outcome. In accordance with the Breast Imaging Reporting & Data System (BI-RADS), it should be visually classified into four classes. The susceptibility of such an assessment to inter-reader variability highlights the urgent need for a standardized classification algorithm. In this retrospective study, the first post-contrast subtraction images for 27 healthy female subjects were included. The BPE was classified slice-wise by two expert radiologists. The extraction of radiomic features from segmented BPE was followed by dataset splitting and dimensionality reduction. The latent representations were then utilized as inputs to a deep neural network classifying BPE into BI-RADS classes. The network’s predictions were elucidated at the radiomic feature level with Shapley values. The deep neural network achieved a BPE classification accuracy of 84 ± 2% (p-value < 0.00001). Most of the misclassifications involved adjacent classes. Different radiomic features were decisive for the prediction of each BPE class underlying the complexity of the decision boundaries. A highly precise and explainable pipeline for BPE classification was achieved without user- or algorithm-dependent radiomic feature selection.
Design and Evaluation of ScanCap: A Low-Cost, Reusable Tethered Capsule Endoscope with Blue-Green Illumination Imaging for Unsedated Screening and Early Detection of Barrett’s Esophagus
Authors: Hicheri Cheima , Azimuddin Ahad M. , Kortum Alex , Bailey Joseph , Tang Yubo , Schwarz Richard A. , Rosen Daniel , Jain Shilpa , Mansour Nabil M. , Groth Shawn , Vasavada Shaleen , Rao Ashwin , Maliga Adrianna , Gallego Leslie , Carns Jennifer , Anandasabapathy Sharmila , Richards-Kortum Rebecca
Published: May 2024

Esophageal carcinoma is the sixth-leading cause of cancer death worldwide. A precursor to esophageal adenocarcinoma (EAC) is Barrett’s Esophagus (BE). Early-stage diagnosis and treatment of esophageal neoplasia (Barrett’s with high-grade dysplasia/intramucosal cancer) increase the five-year survival rate from 10% to 98%. BE is a global challenge; however, current endoscopes for early BE detection are costly and require extensive infrastructure for patient examination and sedation. We describe the design and evaluation of the first prototype of ScanCap, a high-resolution optical endoscopy system with a reusable, low-cost tethered capsule, designed to provide high-definition, blue-green illumination imaging for the early detection of BE in unsedated patients. The tethered capsule (12.8 mm diameter, 35.5 mm length) contains a color camera and rotating mirror and is designed to be swallowed; images are collected as the capsule is retracted manually via the tether. The tether provides electrical power and illumination at wavelengths of 415 nm and 565 nm and transmits data from the camera to a tablet. The ScanCap prototype capsule was used to image the oral mucosa in normal volunteers and ex vivo esophageal resections; images were compared to those obtained using an Olympus CV-180 endoscope. Images of superficial capillaries in intact oral mucosa were clearly visible in ScanCap images. Diagnostically relevant features of BE, including irregular Z-lines, distorted mucosa, and dilated vasculature, were clearly visible in ScanCap images of ex vivo esophageal specimens.
Irisin Ameliorate Acute Pancreatitis and Acinar Cell Viability through Modulation of the Unfolded Protein Response (UPR) and PPARγ-PGC1α-FNDC5 Pathways
Authors: Horwitz Avital , Birk Ruth
Published: May 2024

Acute pancreatitis (AP) entails pancreatic inflammation, tissue damage and dysregulated enzyme secretion, including pancreatic lipase (PL). The role of irisin, an anti-inflammatory and anti-apoptotic cytokine, in AP and exocrine pancreatic stress is unclear. We have previously shown that irisin regulates PL through the PPARγ-PGC1α-FNDC5 pathway. In this study, we investigated irisin and irisin’s pathway on AP in in vitro (AR42J-B13) and ex vivo (rat primary acinar) models using molecular, biochemical and immunohistochemistry methodology. Pancreatitis induction (cerulein (cer)) resulted in a significant up-regulation of the PPARγ-PGC1α-FNDC5 axis, PL expression and secretion and endoplasmic reticulum (ER) stress unfolded protein response (UPR) signal-transduction markers (CHOP, XBP-1 and ATF6). Irisin addition in the cer-pancreatitis state resulted in a significant down-regulation of the PPARγ-PGC1α-FNDC5 axis, PPARγ nucleus-translocation and inflammatory state (TNFα and IL-6) in parallel to diminished PL expression and secretion (in vitro and ex vivo models). Irisin addition up-regulated the expression of pro-survival UPR markers (ATF6 and XBP-1) and reduced UPR pro-apoptotic markers (CHOP) under cer-pancreatitis and induced ER stress (tunicamycin), consequently increasing cells viability. Irisin’s pro-survival effect under cer-pancreatitis state was abolished under PPARγ inhibition. Our findings suggest irisin as a potential therapeutic option for AP via its ability to up-regulate pro-survival UPR signals and activate the PPARγ-PGC1α-FNDC5 pathway.
Adding Genetics to the Risk Factors Model Improved Accuracy for Detecting Visual Field Progression in Newly Diagnosed Exfoliation Glaucoma Patients
Authors: Ayala Marcelo
Published: May 2024

Background: This study aims to determine whether including genetics as a risk factor for progression will improve the accuracy of the models used in newly diagnosed exfoliation glaucoma patients. Methods: This was a prospective cohort study. This study included only patients who were newly diagnosed with exfoliation glaucoma and received treatment upon inclusion. Blood samples were taken from all patients at inclusion to test for the single nucleotide polymorphisms (SNPs) LOXL-1 rs2165241 and rs1048661. Results: This study found that the frequency of SNPs, as well as intraocular pressure (IOP), mean deviation (MD), and visual field index (VFI) values at diagnosis, were significant predictors of visual field deterioration (p ≤ 0.001). This study showed that interaction terms, including SNPs, were highly significant (p ≤ 0.001). Furthermore, logistic regression analysis also showed highly significant results for interaction terms when SNPs were included (p ≤ 0.001). Finally, the area under the curve (AUC) analysis showed an increased value of around 10–20% when SNPs were included. Conclusions: Adding genetic factors to the well-known clinical risk factors can increase the accuracy of models for predicting visual field deterioration in exfoliation glaucoma patients. However, further studies are needed to investigate the role of other genes in this process.
Association of Circulating Markers of Microbial Translocation and Hepatic Inflammation with Liver Injury in Patients with Type 2 Diabetes
Authors: Gobejishvili Leila , Vatsalya Vatsalya , Avila Diana V. , Feygin Yana B. , McClain Craig J. , Mokshagundam Sriprakash , Barve Shirish
Published: May 2024

Background: Virtually the entire spectrum of liver disease is observed in association with type 2 diabetes mellitus (T2DM); indeed, T2DM is now the most common cause of liver disease in the U.S. We conducted a pilot study to investigate the relevance of increased microbial translocation and systemic inflammation in the development of liver injury in patients with T2DM. Methods: Patients with T2DM (n = 17) and non-diabetic controls (NDC; n = 11) aged 25–80 yrs. participated in this study. Serum levels of endotoxin, calprotectin, soluble CD14 and CD163, and several inflammatory cytokines were measured. In addition to standard liver injury markers, ALT and AST, novel serum markers of liver injury, keratin 18 (K-18) M30 (apoptosis-associated caspase-cleaved keratin 18), and M65 (soluble keratin 18) were evaluated. Statistical analyses were performed using the Mann–Whitney test to assess differences between study groups. Pearson’s correlation analysis was performed to determine the strength of association between two variables using GraphPad Prism 9.5.0 software. Results: Patients with T2DM had significantly higher levels of sCD14 in comparison to NDC, suggesting an increase in gut permeability, microbial translocation, and monocyte/macrophage activation. Importantly, relevant to the ensuing inflammatory responses, the increase in sCD14 in patients with T2DM was accompanied by a significant increase in sCD163, a marker of hepatic Kupffer cell activation and inflammation. Further, a positive correlation was observed between sCD163 and endotoxin and sCD14 in T2DM patients but not in NDC. In association with these changes, keratin 18 (K-18)-based serum markers (M65 and M30) that reflect hepatocyte death were significantly higher in the T2DM group indicating ongoing liver injury. Notably, both M65 and M30 levels correlated with sCD14 and sCD163, suggesting that immune cell activation and hepatic inflammation may be linked to the development of liver injury in T2DM. Conclusions: These findings suggest that the pathogenic changes in the gut–liver axis, marked by increased microbial translocation, may be a major component in the etiology of hepatocyte inflammation and injury in patients with T2DM. However, larger longitudinal studies, including histological evidence, are needed to confirm these observations.
Neuron Circuit Based on a Split-gate Transistor with Nonvolatile Memory for Homeostatic Functions of Biological Neurons
Authors: Kim Hansol , Woo Sung Yun , Kim Hyungjin
Published: May 2024

To mimic the homeostatic functionality of biological neurons, a split-gate field-effect transistor (S-G FET) with a charge trap layer is proposed within a neuron circuit. By adjusting the number of charges trapped in the Si3N4 layer, the threshold voltage (Vth) of the S-G FET changes. To prevent degradation of the gate dielectric due to program/erase pulses, the gates for read operation and Vth control were separated through the fin structure. A circuit that modulates the width and amplitude of the pulse was constructed to generate a Program/Erase pulse for the S-G FET as the output pulse of the neuron circuit. By adjusting the Vth of the neuron circuit, the firing rate can be lowered by increasing the Vth of the neuron circuit with a high firing rate. To verify the performance of the neural network based on S-G FET, a simulation of online unsupervised learning and classification in a 2-layer SNN is performed. The results show that the recognition rate was improved by 8% by increasing the threshold of the neuron circuit fired.
Spall Repair Patch Health Monitoring System Using BIM and IoT
Authors: Kim Chaehyeon , Cho Junhwi , Kim Jinhyo , Song Yooseob , Kang Julian , Yeon Jaeheum
Published: May 2024

Concrete infrastructure is vulnerable to damage such as spalling. Spalling leads to chloride penetration, which causes internal corrosion, weakens structural stability and durability, and increases the likelihood of additional cracks or damage, consequently necessitating repair. Existing repair methods do not consider the factors that affect damage in the repaired areas, so additional damage can occur, making monitoring necessary to identify these factors. Nevertheless, existing studies have not adequately addressed the monitoring of internal conditions within concrete, making it difficult to manage such damage effectively. Accordingly, in this study, a monitoring system was developed using building information modeling (BIM) and the Internet of Things (IoT) to better identify internal changes in concrete pavements. Employing embedded sensors to measure temperature, humidity, and stress within the concrete, our system uses Dynamo scripts for real-time data visualization within BIM. Validated against the ASTM D8292 standard, this system captures and analyzes environmental impacts on concrete. This integration facilitates the detection of internal changes, allowing for the real-time visualization of these impacts. This study can help establish repair plans by identifying factors affecting concrete, contributing to preventive maintenance, potentially reducing maintenance costs, and enhancing the sustainability of concrete infrastructures.
Therapeutic Targets in Advanced Penile Cancer: From Bench to Bedside
Authors: Pagliaro Lance C. , Tekin Burak , Gupta Sounak , Herrera Hernandez Loren
Published: May 2024

Discovery of effective systemic therapies for patients with advanced penile cancer has been slow to occur. Comprehensive genomic profiling from several studies shed light on the molecular oncogenesis of penile squamous cell carcinoma (PSCC) and differences between HPV-related and unrelated tumors. While these two subsets of PSCC appear distinct in their biology, there are not yet specific treatment strategies recommended on that basis. Cell surface proteins have been identified that may potentially serve as drug targets for monoclonal antibodies or small molecule inhibitors. Here, we review some of the new biological insights regarding PSCC that could lead to improved therapies, as well as the related clinical trials recently completed or in progress. We conclude that antibody-drug conjugates are especially promising, as are the combinations of immune checkpoint inhibitors with other types of drugs.
Stereotactic Body Radiotherapy as a Curative Treatment for De Novo Mucosal Carcinoma of the Head and Neck: A Feasible Alternative Option for Fragile Patients with Small Lesion: A Systematic Review
Authors: Strojan Primož , Kokalj Marko , Plavc Gaber , Ng Sweet Ping , Nuyts Sandra , Chiesa-Estomba Carlos M. , Eisbruch Avraham , de Bree Remco , Chow James C. H. , Mäkitie Antti A. , Lopez Fernando , Saba Nabil F. , Ferlito Alfio
Published: May 2024

Stereotactic body radiotherapy (SBRT) is characterized by a high dose per fraction, well-defined small targets, superior dose conformity, and a steep off-target dose gradient. A literature search was conducted to examine the experience with SBRT as a curative treatment for newly diagnosed mucosal carcinoma of the head and neck (MCHN). Four retrospective case series and one prospective phase I clinical trial published between 2012 and 2020 described 124 patients. SBRT was mainly performed in older patients with different tumor sites. The median size of the planning target volumes ranged from 5.3 to 41 cm3. Different approaches were used to create margins. In two studies, limited elective nodal irradiation was performed. The equivalent doses used were 60–83.33 Gy delivered in five fractions. Considerable heterogeneity was observed in the radiation dose specification. The incidence of grade ≥3 late toxicity was 0–8.3%, with local and regional control ranging from 73% to 100%. Improved or stable quality of life after SBRT was reported in two studies. Curative-intent SBRT for de novo MCHN appears to be an effective and relatively safe treatment for small tumor targets, preferably without concomitant elective tissue irradiation. Standardization of SBRT practice and well-designed prospective clinical trials are needed to better define the role of SBRT in this setting.
Spatial Distribution of Recurrence and Long-Term Toxicity Following Dose Escalation to the Dominant Intra-Prostatic Nodule for Intermediate–High-Risk Prostate Cancer: Insights from a Phase I/II Study
Authors: Cloître Minna , Benkhaled Sofian , Boughdad Sarah , Schaefer Niklaus , Prior John O. , Zeverino Michele , Berthold Dominik , Tawadros Thomas , Meuwly Jean-Yves , Martel Paul , Rohner Chantal , Heym Leonie , Duclos Frederic , Vallet Véronique , Valerio Massimo , Bourhis Jean , Herrera Fernanda
Published: May 2024

Objectives: We investigated spatial patterns between primary and recurrent tumor sites and assessed long-term toxicity after dose escalation stereotactic body radiation therapy (SBRT) to the dominant intra-prostatic nodule (DIN). Materials and methods: In 33 patients with intermediate–high-risk prostate cancer (PCa), doses up to 50 Gy were administered to the DIN. Recurrence sites were determined and compared to the original tumor development sites through multiparametric MRI and 68Ga-labeled prostate-specific membrane antigen (PSMA) positron emission tomography/computed tomography (68Ga-PSMA-PET/CT) images. Overlap rates, categorized as 75% or higher for full overlap, and 25–74% for partial overlap, were assessed. Long-term toxicity is reported. Results: All patients completed treatment, with only one receiving concomitant androgen deprivation therapy (ADT). Recurrences were diagnosed after a median of 33 months (range: 17–76 months), affecting 13 out of 33 patients (39.4%). Intra-prostatic recurrences occurred in 7 patients (21%), with ≥75% overlap in two, a partial overlap in another two, and no overlap in the remaining three patients. Notably, five patients with intra-prostatic recurrences had synchronous bone and/or lymph node metastases, while six patients had isolated bone or lymph node metastasis without intra-prostatic recurrences. Extended follow-up revealed late grade ≥ 2 GU and GI toxicity in 18% (n = 6) and 6% (n = 2) of the patients. Conclusions: Among patients with intermediate–high-risk PCa undergoing focal dose-escalated SBRT without ADT, DIN recurrences were infrequent. When present, these recurrences were typically located at the original site or adjacent to the initial tumor. Conversely, relapses beyond the DIN and in extra-prostatic (metastatic) sites were prevalent, underscoring the significance of systemic ADT in managing this patient population. Advances in knowledge: Focal dose-escalated prostate SBRT prevented recurrences in the dominant nodule; however, extra-prostatic recurrence sites were frequent.
Beam Position Projection Algorithms in Proton Pencil Beam Scanning
Authors: Nesteruk Konrad P. , Bradley Stephen G. , Kooy Hanne M. , Clasie Benjamin M.
Published: May 2024

Beam position uncertainties along the beam trajectory arise from the accelerator, beamline, and scanning magnets (SMs). They can be monitored in real time, e.g., through strip ionization chambers (ICs), and treatments can be paused if needed. Delivery is more reliable and accurate if the beam position is projected from monitored nozzle parameters to the isocenter, allowing for accurate online corrections to be performed. Beam position projection algorithms are also used in post-delivery log file analyses. In this paper, we investigate the four potential algorithms that can be applied to all pencil beam scanning (PBS) nozzles. For some combinations of nozzle configurations and algorithms, however, the projection uses beam properties determined offline (e.g., through beam tuning or technical commissioning). The best algorithm minimizes either the total uncertainty (i.e., offline and online) or the total offline uncertainty in the projection. Four beam position algorithms are analyzed (A1–A4). Two nozzle lengths are used as examples: a large nozzle (1.5 m length) and a small nozzle (0.4 m length). Three nozzle configurations are considered: IC after SM, IC before SM, and ICs on both sides. Default uncertainties are selected for ion chamber measurements, nozzle entrance beam position and angle, and scanning magnet angle. The results for other uncertainties can be determined by scaling these results or repeating the error propagation. We show the propagation of errors from two locations and the SM angle to the isocenter for all the algorithms. The best choice of algorithm depends on the nozzle length and is A1 and A3 for the large and small nozzles, respectively. If the total offline uncertainty is to be minimized (a better choice if the offline uncertainty is not stable), the best choice of algorithm changes to A1 for the small nozzle for some hardware configurations. Reducing the nozzle length can help to reduce the gantry size and make proton therapy more accessible. This work is important for designing smaller nozzles and, consequently, smaller gantries. This work is also important for log file analyses.
Implementation of HER2 Testing in Endometrial Cancer, a Summary of Real-World Initial Experience in a Large Tertiary Cancer Center
Authors: Plotkin Anna , Olkhov-Mitsel Ekaterina , Huang Weei-Yuarn , Nofech-Mozes Sharon
Published: May 2024

HER2-targeted therapies have transformed the management of advanced or recurrent serous endometrial cancer (EC), leading to an increased clinical demand for HER2 testing. Despite its adoption in select academic centers, the global extent of such tumor testing is unclear. In this study, we report on the initial two-year experience of HER2 testing at a major academic center with a reference gynecologic oncology service and biomarker reference laboratory. All patients who underwent HER2 testing based on physician discretion, reflex HER2 testing, and reference laboratory requests were included. From February 2021 to October 2023, HER2 testing was performed on 192 tumor tissue samples from 180 EC patients. Serous carcinoma constituted 52% of samples, reflecting diagnostic challenges and limited therapeutic options for advanced EC. HER2 positivity was found in 28% of all cases and 30% of p53-aberrant cases. An immunohistochemistry (IHC) score of 3+ was found in 15% of samples, while IHC 2+ was found in 45% (13% IHC 2+/ISH+ and 32% IHC 2+/ISH−). The newly identified ‘HER2-low’ category comprised 46% of the samples. Heterogeneity was noted in 42% of HER2-positive cases, with complex patterns in 3%. NGS and HER2 IHC-FISH showed a 24% discordance, attributed to intratumoral heterogeneity, tumor cellularity, a small number of amplified cells, and the HER2/CEP17 ratio near the cut-off. This study offers real-world insights into HER2 testing in EC, highlighting the challenges and underscoring the need for standardized guidelines in specimen handling, proficiency testing, and scoring criteria to enhance patient management and therapeutic decision-making.
Fertility-Preserving Treatments and Patient- and Parental Satisfaction on Fertility Counseling in a Cohort of Newly Diagnosed Boys and Girls with Childhood Hodgkin Lymphoma
Authors: Drechsel Katja C. E. , IJgosse Irene M. , Slaats Sofie , Raasen Lisanne , Stoutjesdijk Francis S. , van Dulmen-den Broeder Eline , Wallace W. Hamish , Beishuizen Auke , Körholz Dieter , Mauz-Körholz Christine , Cepelova Michaela , Uyttebroeck Anne , Ronceray Leila , Kaspers Gertjan J. L. , Broer Simone L. , Veening Margreet A.
Published: May 2024

Purpose: The purpose of this study is to evaluate the use of fertility-preserving (FP) treatments and fertility counseling that was offered in a cohort of newly diagnosed children with classical Hodgkin lymphoma (cHL). Methods: In this observational study, boys and girls with cHL aged ≤ 18 years with scheduled treatment according to the EuroNet-PHL-C2 protocol were recruited from 18 sites (5 countries), between January 2017 and September 2021. In 2023, a subset of Dutch participants (aged ≥ 12 years at time of diagnosis) and parents/guardians were surveyed regarding fertility counseling. Results: A total of 101 boys and 104 girls were included. Most post-pubertal boys opted for semen cryopreservation pre-treatment (85% of expected). Invasive FP treatments were occasionally chosen for patients at a relatively low risk of fertility based on scheduled alkylating agent exposure (4/5 testicular biopsy, 4/4 oocyte, and 11/11 ovarian tissue cryopreservation). A total of 17 post-menarchal girls (20%) received GnRH-analogue co-treatment. Furthermore, 33/84 parents and 26/63 patients responded to the questionnaire. Most reported receiving fertility counseling (97%/89%). Statements regarding the timing and content of counseling were generally positive. Parents and patients considered fertility counseling important (94%/87% (strongly agreed) and most expressed concerns about (their child’s) fertility (at diagnosis 69%/46%, at present: 59%/42%). Conclusion: Systematic fertility counseling is crucial for all pediatric cHL patients and their families. FP treatment should be considered depending on the anticipated risk and patient factors. We encourage the development of a decision aid for FP in pediatric oncology.
Effect of the Metal of a Metallic Ionic Liquid (-butyl-methylimidazolium tetrachloroferrate) on the Oxidation of Hydrazine
Authors: Brockmann Marcela , Navarro Freddy , Ibarra José , León Constanza , Armijo Francisco , Aguirre María Jesús , Ramírez Galo , Arce Roxana
Published: May 2024

This work investigates the electrocatalytic properties of carbon paste electrodes (CPEs) modified with ionic liquids (IL) and metallic ionic liquid (ILFe) for the hydrazine oxidation reaction (HzOR). The results indicate that ILFe significantly enhances the catalytic activity of the electrode, exhibiting catalysis towards hydrazine oxidation, reducing overpotential, and increasing reaction current. It is determined that the HzOR on the MWCNT/MO/ILFe electrode involves the transfer of four electrons, with high selectivity for nitrogen formation. Additionally, ILFe is observed to improve the wettability of the electrode surface, increasing its capacitance and reaction efficiency. This study highlights the advantages of ILFe-modified CPEs in terms of simplicity, cost-effectiveness, and improved performance for electrochemical applications, demonstrating how the ionic liquid catalyzes hydrazine oxidation despite its lower conductivity.
Structure-Based Discovery of Potential HPV E6 and EBNA1 Inhibitors: Implications for Cervical Cancer Treatment
Authors: Broni Emmanuel , Ashley Carolyn N. , Velazquez Miriam , Sakyi Patrick O. , Kwofie Samuel K. , Miller Whelton A.
Published: May 2024

Cervical cancer is the fourth most diagnosed cancer and the fourth leading cause of cancer death in women globally. Its onset and progression have been attributed to high-risk human papillomavirus (HPV) types, especially 16 and 18, while the Epstein–Barr virus (EBV) is believed to also significantly contribute to cervical cancer growth. The E6 protein associated with high-risk HPV strains, such as HPV16 and HPV18, is known for its role in promoting cervical cancer and other anogenital cancers. E6 proteins contribute to the malignant transformation of infected cells by targeting and degrading tumor suppressor proteins, especially p53. On the other hand, EBV nuclear antigen 1 (EBNA1) plays a crucial role in the maintenance and replication of the EBV genome in infected cells. EBNA1 is believed to increase HPV E6 and E7 levels, as well as c-MYC, and BIRC5 cellular genes in the HeLa cell line, implying that HPV/EBV co-infection accelerates cervical cancer onset and growth. Thus, the E6 and EBNA1 antigens of HPV and EBV, respectively, are attractive targets for cervical cancer immunotherapy. This study, therefore, virtually screened for potential drug candidates with good binding affinity to all three oncoviral proteins, HPV16 E6, HPV18 E6, and EBNA1. The compounds were further subjected to ADMET profiling, biological activity predictions, molecular dynamics (MD) simulations, and molecular mechanics Poisson–Boltzmann surface area (MM/PBSA) calculations. A total of six compounds comprising ZINC000013380012, ZINC000070454124, ZINC000014588133, ZINC000085568136, ZINC000095909247, and ZINC000085597263 demonstrated very strong affinity (≤−60 kJ/mol) to the three oncoviral proteins (EBNA1, HPV16 E6, and HPV18 E6) after being subjected to docking, MD, and MM/PBSA. These compounds demonstrated relatively stronger binding than the controls used, inhibitors of EBNA1 (VK-1727) and HPV E6 (baicalein and gossypetin). Biological activity predictions also corroborated their antineoplastic, p53-enhancing, Pin1 inhibitory, and JAK2 inhibitory activities. Further experimental testing is required to validate the ability of the shortlisted compounds to silence the insidious effects of HPV E6 and EBNA1 proteins in cervical cancers.
A Review of Cu3BiS3 Thin Films: A Sustainable and Cost-Effective Photovoltaic Material
Authors: Libório Maxwell Santana , Queiroz José César Augusto de , Sivasankar Sivabalan Maniam , Costa Thercio Henrique de Carvalho , Cunha António Ferreira da , Amorim Carlos de Oliveira
Published: May 2024

The demand for sustainable and cost-effective materials for photovoltaic technology has led to an increasing interest in Cu3BiS3 thin films as potential absorber layers. This review provides a comprehensive overview of the main physical properties, synthesis methods, and theoretical studies of Cu3BiS3 thin films for photovoltaic applications. The high optical absorption coefficient and band gap energy around the optimal 1.4 eV make Cu3BiS3 orthorhombic Wittichenite-phase a promising viable alternative to conventional thin film absorber materials such as CIGS, CZTS, and CdTe. Several synthesis techniques, including sputtering, thermal evaporation, spin coating, chemical bath deposition, and spray deposition, are discussed, highlighting their impact on film quality and photovoltaic performance. Density Functional Theory studies offer insights into the electronic structure and optical properties of Cu3BiS3, aiding in the understanding of its potential for photovoltaic applications. Additionally, theoretical modeling of Cu3BiS3-based photovoltaic cells suggests promising efficiencies, although experimental challenges remain to be addressed. Overall, this review underscores the potential of CBS thin films as sustainable and cost-effective materials for future PV technology while also outlining the ongoing research efforts and remaining challenges in this field.
Molecular Dynamics Study of the Deformation Behavior and Strengthening Mechanisms of Cu/Graphene Composites under Nanoindentation
Authors: Ren Guangan , Zhou Cong , Hu Yongle , Wang Li , Fang Jingzhong , Li Yejun , Wang Yi , Liu Jian , Zhang Mingjun , Tong Yonggang
Published: May 2024

The mechanical performance of pure copper can be significantly strengthened by adding graphene without greatly sacrificing its electrical and thermal conductivity. However, it is difficult to observe the deformation behavior of Cu/graphene composites efficiently and optically using experiments due to the extremely small graphene size. Herein, Cu/graphene composites with different graphene positions and layers were built to investigate the effect of these factors on the mechanical performance of the composites and the deformation mechanisms using molecular dynamics simulations. The results showed that the maximum indentation force and hardness of the composites decreased significantly with an increase in the distance from graphene to the indentation surface. Graphene strengthened the mechanical properties of Cu/graphene composites by hindering the slip of dislocations. As the graphene layers increased, the strengthening effect became more pronounced. With more graphene layers, dislocations within the Cu matrix were required to overcome higher stress to be released towards the surface; thus, they had to store enough energy to allow more crystalline surfaces to slip, resulting in more dislocations being generated.