bluuebunny/crossref_metadata_2025 huggingface dataset:
The Double Face of miR-708: A Pan-Cancer Player with Dissociative Identity Disorder
Authors: Carvalho de Oliveira Jaqueline , Mathias Carolina , Oliveira Verônica Cristina , Pezuk Julia Alejandra , Brassesco María Sol
Published: December 2022

Over the last decades, accumulating evidence has shown tumor-dependent profiles of miR-708, being either up- or downregulated, and thus, acting as a “Janus” regulator of oncogenic pathways. Herein, its functional duality was assessed through a thorough review of the literature and further validation in silico using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. In the literature, miR-708 was found with an oncogenic role in eight tumor types, while a suppressor tumor role was described in seven cancers. This double profile was also found in TCGA and GEO databases, with some tumor types having a high expression of miR-708 and others with low expression compared with non-tumor counterparts. The investigation of validated targets using miRBase, miRTarBase, and miRecords platforms, identified a total of 572 genes that appeared enriched for PI3K-Akt signaling, followed by cell cycle control, p53, Apellin and Hippo signaling, endocrine resistance, focal adhesion, and cell senescence regulations, which are all recognized contributors of tumoral phenotypes. Among these targets, a set of 15 genes shared by at least two platforms was identified, most of which have important roles in cancer cells that influence either tumor suppression or progression. In a clinical scenario, miR-708 has shown to be a good diagnostic and prognosis marker. However, its multitarget nature and opposing roles in diverse human tumors, aligned with insufficient experimental data and the lack of proper delivery strategies, hamper its potential as a sequence-directed therapeutic.
Dual Molecular Diagnoses of Recessive Disorders in a Child from Consanguineous Parents: Case Report and Literature Review
Authors: Correia-Costa Gabriela Roldão , dos Santos Ana Mondadori , de Leeuw Nicole , Rigatto Sumara Zuanazi Pinto , Belangero Vera Maria Santoro , Steiner Carlos Eduardo , Gil-da-Silva-Lopes Vera Lúcia , Vieira Társis Paiva
Published: December 2022

The widespread use of whole exome sequencing (WES) resulted in the discovery of multilocus pathogenic variations (MPV), defined as two or more distinct or overlapping Mendelian disorders occurring in a patient, leading to a blended phenotype. In this study, we report on a child with autosomal recessive primary microcephaly-5 (MCPH5) and nephropathic cystinosis. The proband is the first child of consanguineous parents, presenting a complex phenotype including neurodevelopmental delay, microcephaly, growth restriction, significant delay of bone maturation, lissencephaly, and abnormality of neuronal migration, photophobia, and renal tubular acidosis. WES revealed two pathogenic and homozygous variants: a c.4174C>T variant in the ASPM gene and a c.382C>T variant in the CTNS gene, explaining the complex phenotype. The literature review showed that most of the patients harboring two variants in recessive disease genes are born to consanguineous parents. To the best of our knowledge, the patient herein described is the first one harboring pathogenic variants in both the ASPM and CTNS genes. These findings highlight the importance of searching for MPV in patients with complex phenotypes investigated by genome-wide testing methods, especially for those patients born to consanguineous parents.
Identification of Novel Genes Associated with Fish Skeletal Muscle Adaptation during Fasting and Refeeding Based on a Meta-Analysis
Authors: Perez Érika , Cury Sarah , Zanella Bruna , Carvalho Robson , Duran Bruno , Dal-Pai-Silva Maeli
Published: December 2022

The regulation of the fish phenotype and muscle growth is influenced by fasting and refeeding periods, which occur in nature and are commonly applied in fish farming. However, the regulators associated with the muscle responses to these manipulations of food availability have not been fully characterized. We aimed to identify novel genes associated with fish skeletal muscle adaptation during fasting and refeeding based on a meta-analysis. Genes related to translational and proliferative machinery were investigated in pacus (Piaractus mesopotamicus) subjected to fasting (four and fifteen days) and refeeding (six hours, three and fifteen days). Our results showed that different fasting and refeeding periods modulate the expression of the genes mtor, rps27a, eef1a2, and cdkn1a. These alterations can indicate the possible protection of the muscle phenotype, in addition to adaptive responses that prioritize energy and substrate savings over cell division, a process regulated by ccnd1. Our study reveals the potential of meta-analysis for the identification of muscle growth regulators and provides new information on muscle responses to fasting and refeeding in fish that are of economic importance to aquaculture.
Roles of Polycomb Complexes in the Reconstruction of 3D Genome Architecture during Preimplantation Embryonic Development
Authors: Yu Longtao , Shen Hengxiang , Lyu Xiaowen
Published: December 2022

The appropriate deployment of developmental programs depends on complex genetic information encoded by genomic DNA sequences and their positioning and contacts in the three-dimensional (3D) space within the nucleus. Current studies using novel techniques including, but not limited to, Hi-C, ChIA-PET, and Hi-ChIP reveal that regulatory elements (Res), such as enhancers and promoters, may participate in the precise regulation of expression of tissue-specific genes important for both embryogenesis and organogenesis by recruiting Polycomb Group (PcG) complexes. PcG complexes usually poise the transcription of developmental genes by forming Polycomb bodies to compact poised enhancers and promoters marked by H3K27me3 in the 3D space. Additionally, recent studies have also uncovered their roles in transcriptional activation. To better understand the full complexities in the mechanisms of how PcG complexes regulate transcription and long-range 3D contacts of enhancers and promoters during developmental programs, we outline novel insights regarding PcG-associated dramatic changes in the 3D chromatin conformation in developmental programs of early embryos and naïve-ground-state transitions of pluripotent embryonic stem cells (ESCs), and highlight the distinct roles of unique and common subunits of canonical and non-canonical PcG complexes in shaping genome architectures and transcriptional programs.
Identification of Genetic Effects of ACADVL and IRF6 Genes with Milk Production Traits of Holstein Cattle in China
Authors: Peng Peng , Liu Yanan , Zheng Weijie , Han Bo , Wang Kun , Sun Dongxiao
Published: December 2022

With the development of high-throughput sequencing, RNA sequencing has been widely used in the identification of candidate genes for complex traits in livestock, and the functional genes and mutations with large genetic effects on milk production traits can provide molecular information for marker-assisted selection to increase the selection accuracy and accelerate genetic gain in dairy cattle. Our previous study on the liver transcriptome of Holstein cows found that acyl-CoA dehydrogenase (ACADVL) and interferon regulatory factor 6 (IRF6) are differentially expressed between dry and peak lactation periods, as well as that they are involved in lipid metabolism and the proliferation and differentiation of mammary epithelial cells. Thus, the two genes were considered candidates for milk traits. Hence, this study further collected 1186 Holstein cows from 110 sire families to investigate their genetic associations with milk yield and composition traits. By resequencing the entire exons and 2000 bp of the 5′ and 3′ flanking regions of the two genes, we identified eight SNPs in ACADVL and eight SNPs in IRF6. Subsequent single-locus association analyses showed that the eight SNPs in ACADVL were all significantly associated with milk fat yield, fat percentage, and protein yield (p values ≤ 0.0001–0.0414), and the eight SNPs in IRF6 were associated with milk, fat, and protein yields in the first or second lactation (p values ≤ 0.0001–0.0467). Using Haploview 4.2, one haplotype block with eight of the SNPs in ACADVL (D’ = 0.99–1.00) and two haplotype blocks in IRF6 with three of the SNPs in each were observed (D’ = 0.98–1.00). Similarly, the haplotype combinations of ACADVL were significantly associated with milk yield, fat percentage, fat yield, and protein yield in the two lactations (p values ≤ 0.0001–0.0125), and those of IRF6 were associated with five milk traits (p values ≤ 0.0001–0.0263). Furthermore, with the JASPAR software, it was predicted that the SNPs 19:g.26933503T>C in ACADVL and 16:g.73501985G>A in IRF6 changed the transcription factor binding sites of ZEB1, PLAGL2, and RHOXF1, implying their impacts on the expressions of the corresponding genes. Our findings demonstrated that the ACADVL and IRF6 genes have significant genetic effects on milk yield and composition traits, and the valuable SNPs might be used as genetic markers for genomic selection programs in dairy cattle.
MDSN: A Module Detection Method for Identifying High-Order Epistatic Interactions
Authors: Sun Yan , Gu Yijun , Ren Qianqian , Li Yiting , Shang Junliang , Liu Jin-Xing , Guan Boxin
Published: December 2022

Epistatic interactions are referred to as SNPs (single nucleotide polymorphisms) that affect disease development and trait expression nonlinearly, and hence identifying epistatic interactions plays a great role in explaining the pathogenesis and genetic heterogeneity of complex diseases. Many methods have been proposed for epistasis detection; nevertheless, they mainly focus on low-order epistatic interactions, two-order or three-order for instance, and often ignore high-order interactions due to computational burden. In this paper, a module detection method called MDSN is proposed for identifying high-order epistatic interactions. First, an SNP network is constructed by a construction strategy of interaction complementary, which consists of low-order SNP interactions that can be obtained from fast computations. Then, a node evaluation measure that integrates multi-topological features is proposed to improve the node expansion algorithm, where the importance of a node is comprehensively evaluated by the topological characteristics of the neighborhood. Finally, modules are detected in the constructed SNP network, which have high-order epistatic interactions associated with the disease. The MDSN was compared with four state-of-the-art methods on simulation datasets and a real Age-related Macular Degeneration dataset. The results demonstrate that MDSN has higher performance on detecting high-order interactions.
Morphological Characteristics and Comparative Chloroplast Genome Analyses between Red and White Flower Phenotypes of Pyracantha fortuneana (Maxim.) Li (Rosaceae), with Implications for Taxonomy and Phylogeny
Authors: Ding Shi-Xiong , Li Jia-Chen , Hu Ke , Huang Zi-Jian , Lu Rui-Sen
Published: December 2022

Pyracantha fortuneana (Maxim.) Li (Rosaceae), commonly known as Chinese firethorn, is an evergreen shrub with high nutritional, medicinal, and horticultural importance. This species typically has white flowers, but a rare red flower phenotype has been found in very few wild populations in western Hubei, China, showing great ornamental potential. In this study, the complete chloroplast genome of the red flower phenotype of P. fortuneana was reported for the first time, using high-throughput sequencing technology. The complete chloroplast genome was 160,361 bp in length and showed a typical quadripartite structure with a pair of inverted repeat (IR) regions (26,350 bp) separated by a large single-copy (LSC) region (88,316 bp) and a small single-copy (SSC) region (19,345 bp). A total of 131 functional genes were annotated in this chloroplast genome, including 86 protein-coding genes (PCGs), eight rRNA genes, and 37 tRNA genes. Comparative chloroplast genome analyses revealed that high genome similarity existed not only between red and white flower phenotypes of P. fortuneana, but also among Pyracantha species. No evidence for positive selection was found in any PCG, suggesting the evolutionary conservation of Pyracantha chloroplast genomes. Furthermore, four mutational hotspots (trnG-trnR-atpA, psbZ-trnG-trnfM-rps14, ycf3-trnS-rps4, and ndhF-rpl32) with π > 0.004 were identified as potential molecular markers for Pyracantha species. Phylogenomic analysis strongly supported that the red flower phenotype of P. fortuneana was nested within the common white flower phenotype. Based on both morphological and molecular evidence, we suggest that the red flower phenotype of P. fortuneana could be considered as a new forma. Overall, the availability of these genetic resources will not only offer valuable information for further studies on molecular taxonomy, phylogeny, and population genetics of Pyracantha species but also could be used as potential genetic resources for Chinese firethorn breeding.
Spatial Distribution of Benthic Foraminifera in the Neretva Channel (Croatia Coast): Faunal Response to Environmental Parameters
Authors: Capotondi Lucilla , Bonomo Sergio , Graiani Andrea , Innangi Michele , Innangi Sara , Giglio Federico , Ravaioli Mariangela , Ferraro Luciana
Published: December 2022

Benthic foraminiferal assemblages have been studied at 11 sediment surface samples located in the Neretva Channel covering the delta habitat and the adjacent open sea areas. The major objective of the investigation was to explore the main environmental parameters affecting the benthic foraminifera compositional changes. To this end, a statistical approach was applied that integrates micropaleontological data with physical, geochemical and sedimentological parameters (total organic carbon and grain size). Statistical analyses identified four distinct groups (cluster A1, A2, B1, B2) corresponding to different environmental settings. Cluster A1 groups samples under Neretva river influence and is characterized by Aubignyna perlucida, Nonionella turgida, Eggerelloides scaber and Rectuvigerina sp.; species able to live in organic-matter-rich sediments and in a wide range of oxygen content. Cluster A2 includes samples distant from the fluvial outlet and samples along the NW coast partially influenced by the Neretva river plume. In these environmental conditions, Ammonia beccarii, Bulimina marginata, Nonionella turgida and Textularia sp. resulted as the most characteristic taxa. Cluster B1 distinguishes the deepest stations which are in connection with the open Adriatic Sea. Here Asterigerinata mamilla, Buccella granulata, Cibicides group, Reussella spinulosa and Textularia sp. reach their maximum abundance associated with coarse-grained sediments. Cluster B2 groups samples collected in the inner bay of the southernmost sector of the studied area characterized by silt and clay and a negligible influence by river inputs. The benthic microfauna is principally composed of Miliolids, Porosononion granosum and Textularia sp.
What Quality-of-Life Dimensions Are Most Important to Older Adults from Culturally and Linguistically Diverse Backgrounds Receiving Aged Care Services? An Exploratory Study
Authors: Hutchinson Claire , Cleland Jenny , Walker Ruth , Ratcliffe Julie
Published: December 2022

There is little research on what aspects of quality of life (QoL) are most important to culturally and linguistically diverse (CALD) older adults. This study aimed to identify what QoL dimensions were most important to CALD older adults receiving aged care services, and therefore, how relevant a new six dimensions QoL instrument developed for use in aged care is to this population. A three-stage, mixed-methods study was undertaken. Stage 1: n = 3 focus groups with aged care providers. Stage 2: n = 30 semi-structured interviews with Italian-born older adults in ethno-specific residential aged care. Stage 3: survey of n = 63 older adults from mixed CALD backgrounds receiving community aged care services. Overall, older adults asserted the importance of the six dimensions of the new QoL instrument. The importance of ‘identity’ and ‘purpose and meaning’ were identified via the focus groups; however, the community-based CALD older adults identified these aspects of quality of life as more important than older Italians in residential care. Being in ethno-specific residential aged care where needs relating to language, food, and religion were met and they continued to live with others from their community may have meant that the meeting of cultural needs was more taken for granted.
Consumers’ Channel Preference for Fresh Foods and Its Determinants during COVID-19—Evidence from China
Authors: Pu Xujin , Chai Jingyi , Qi Rongtao
Published: December 2022

The public has been experiencing unprecedented challenges during the COVID-19 pandemic for the past two years. Government measures, such as improvements in offline markets and the encouragement of contactless e-commerce use, have been taken to abate the spread of infection. This study explored whether public channel preferences for fresh foods have changed and aimed to identify potential determinants. Data from 10,708 consumers were obtained by issuing questionnaires, and the binary logic measurement model was used for the empirical analysis to study the core factors that determine consumers’ choice of online and offline purchase channels for fresh food. The results show that, from the perspective of consumers’ personal behavior, consumers who do not pay attention to online evaluations and consumers who do not buy products based on their purchase experience have increased the frequency of online fresh food purchases during the epidemic. Food safety also significantly affects consumers’ choices of purchase channels. Consumers who believe that online fresh foods are safer prefer to purchase fresh food online. Among the factors affecting the performance of online fresh food, consumers concerned about food safety increased the frequency of online purchases, while consumers concerned about the reputation of the platform decreased the frequency of online purchases. These findings can help online and offline retailers better understand consumer needs and then determine their marketing strategies.
Monitoring Stem Water Potential with an Embedded Microtensiometer to Inform Irrigation Scheduling in Fruit Crops
Authors: Lakso Alan N. , Santiago Michael , Stroock Abraham D.
Published: December 2022

The water status of fruit and nut crops is critical to the high productivity, quality and value of these crops. Water status is often estimated and managed with indirect measurements of soil moisture and models of evapotranspiration. However, cultivated trees and vines have characteristics and associated cultural practices that complicate such methods, particularly variable discontinuous canopies, and extensive but low-density, variable root systems with relatively high hydraulic resistance. Direct and continuous measurement of plant water status is desirable in these crops as the plant integrates its unique combination of weather, soil and cultural factors. To measure plant water potential with high temporal sampling rates, a stem-embedded microchip microtensiometer sensor has been developed and tested in several fruit crops for long-term continuous monitoring of stem water potential. Results on several fruit crops in orchards and vineyards have been good to excellent, with very good correlations to the pressure chamber standard method. The primary challenge has been establishing and maintaining the intimate contact with the xylem for long periods of time, with variable stem anatomies, stem growth and wound reactions. Sources of variability in the measurements and utilization of the continuous data stream, in relation to irrigation scheduling, are discussed. Direct continuous and long-term field measurements are possible and provide unique opportunities for both research and farming.
Preharvest Elicitors Spray Improves Antioxidant Activity, Alleviates Chilling Injury, and Maintains Quality in Harvested Fruit
Authors: Gong Di , Bi Yang , Li Yongcai , Wang Yi , Prusky Dov , Alkan Noam
Published: December 2022

Antioxidant activity is an important feature for evaluating fruit quality and tolerance to biotic or abiotic stresses. Moreover, antioxidant activity is involved in chilling injury (CI) response and postharvest quality in fruit. Chemical elicitors can induce systemic acquired resistance in fruit against pathogens, which could partially replace synthetic fungicides. Recently, researchers have found that preharvest sprays with chemical elicitors can improve antioxidant activity, reduce CI, and maintain quality in harvested fruit. In this review, we summarize that preharvest elicitors spray improve antioxidant activity in harvested fruit by promoting antioxidant components biosynthesis as well as antioxidant ability in vitro. Moreover, preharvest elicitors spray alleviates CI in fruit by regulation of membrane lipid metabolism and reactive oxygen species metabolism. In addition, preharvest elicitors spray maintains fruit quality by modulation of respiration and ethylene release. Finally, this review points out the issues existing and proposes an outlook on preharvest elicitors spray to maintain postharvest fruit quality.
Decontamination of Tomato Brown Rugose Fruit Virus-Contaminated Shoe Soles under Practical Conditions
Authors: Ehlers Jens , Nourinejhad Zarghani Shaheen , Kroschewski Bärbel , Büttner Carmen , Bandte Martina
Published: December 2022

Due to its global spread, easy mechanical transmissibility inside greenhouses, and detrimental effects on marketability, Tomato brown rugose fruit virus (ToBRFV) is considered to be one of the biggest threats to tomato production. Regarding such crop epidemics, it is essential to identify all conceivable transmission routes and to interrupt them with effective decontamination strategies. We analyzed the potential efficacy of reliable shoe sole cleaning in combination with a disinfection measure. For this purpose, first, a suspension test was undertaken that involved applying different disinfectants to an infected plant homogenate. This was followed by a simulated carrier test. Finally, shoe-sole decontamination was tested under practical conditions. The extent of decontamination was determined by bioassays of the infectivity of the initial load remaining after treatment. Thereby, necrotic local lesions on the susceptible indicator plant Nicotiana tabacum cv. Xanthi NN were counted. Recommendations for practical applications, based on suspension or simulated carrier tests, are limited in their applicability, since very short contact times between ToBRFV and disinfectants reduce efficacy. Under practical conditions, the approved disinfectant MENNO Florades was able to achieve complete inactivation of the virus in the disinfection mat following mechanical depletion from the shoe soles.
Transcriptional Effects of Rootstock on Scion after Drought: A Case Study of Using MdGH3 RNAi as the Rootstock
Authors: He Jieqiang , Guo Junxing , Jiang Lijuan , An Wenjing , Ma Fengwang , Guan Qingmei , Niu Chundong
Published: December 2022

Drought stress is an important environmental factor limiting apple yield and fruit quality. Previously, we identified GRETCHEN HAGEN3.6 (GH3.6) as a negative regulator of drought stress in apple trees. Using transgenic MdGH3 RNAi (knocking down MdGH3.6 and its five homologs) plants as rootstock can increase drought tolerance, water use efficiency, flowering, and fruit quality of the Fuji scion after drought stress. However, the molecular mechanism behind this phenomenon is still unknown. Here, we performed transcriptome sequencing of the grafted plants (Fuji/GL-3 where Fuji was used as the scion and non-transgenic GL-3 was used as the rootstock, and Fuji/MdGH3 RNAi where MdGH3 RNAi was used as the rootstock) under control and drought conditions. Under control conditions, 667 up-regulated genes and 176 down-regulated genes were identified in the scion of Fuji/MdGH3 RNAi, as compared to the scion of Fuji/GL-3. Moreover, 941 up-regulated genes and 2226 down-regulated genes were identified in the rootstock of MdGH3 RNAi plants relative to GL-3. GO terms of these differentially expressed genes (DEGs) in scion and rootstock showed associations with plant growth, fruit development, and stress responses. After drought stress, 220 up-regulated and 452 down-regulated genes were identified in MdGH3 RNAi rootstock, as compared to GL-3. Significantly enriched GO terms included response to abiotic stimulus, cell division, microtubule-based process, metabolic and biosynthetic process of flavonoid, pigment, and lignin. The comparison between the scion of Fuji/MdGH3 RNAi and Fuji/GL-3 yielded a smaller number of DEGs; however, all of them were significantly enriched in stress-related GO terms. Furthermore, 365 and 300 mRNAs could potentially move from MdGH3 RNAi rootstock to scion under control and drought conditions, respectively, including FIDDLEHEAD (FDH), RESPONSIVE TO DESICCATION 26 (RD26), ARS-binding factor 2 (ABF2), WRKY75, and ferritin (FER). Overall, our work demonstrates the effects of rootstock on scion at the transcriptional level after drought stress and provides theoretical support for further understanding and utilization of MdGH3 RNAi plants.
Program Evaluation of a Workshop on Prairie Strips for Farm Advisors: Framing the Co-Occurring Outcomes of Low Knowledge Acquisition and High Confidence
Authors: Whitehair Rachael , Grudens-Schuck Nancy , Schulte Lisa A.
Published: December 2022

The agricultural conservation practice of prairie strips is new and novel. Prairie strips planted in row crop fields warrants greater adoption because the application decreases erosion; protects water quality; and supports habitat for wildlife and biodiversity, including pollinators. Prairie strips are a vegetative practice composed of diverse, native, and mostly perennial species that, as a community, follow principles of ecological succession; however, they must be managed for success. Farm advisor comprehension of practice characteristics is key for adoption by producers and landowners. This article reports on a developmental evaluation of workshops intended to change farm advisor knowledge, skills, and confidence related to prairie strips management for use in consulting with farmers and landowners. The study used pre-post instruments of knowledge and skill focused on prairie species identification and age of prairie strips planing; pre-then post-end of session questions were asked in a survey to report change in knowledge, skill, and confidence, as well as farm advisor situation. Advisors reported increased confidence, but acquisition of prairie knowledge and skills resulted in flat to lower scores. The paper explores the discrepancy of lower cognitive scores (knowledge and skills) compared to higher confidence. Explanations explore the phenomena of satisficing and perceived self-efficacy to explain the differential.
Damage Caused by Bacchisa medioviolacea Breuning (Coleoptera: Cerambycidae) in Wild Apple (Docynia indica) Orchards in Northwest Vietnam
Authors: Quang Dao Ngoc , Thu Pham Quang , Binh Le Van , Pham Duy Long , Thang Tran Viet , Tong Trang A. , Thu Nguyen Hoai , Thanh Nguyen Van , Thong Nguyen Quoc , Tiep Bui Quang , Dell Bernard
Published: December 2022

The wood-borer Bacchisa medioviolacea Breuning (Coleoptera: Cerambycidae) is identified as a major new pest of Docynia indica (Rosales: Rosaceae) orchards in the northwest mountainous provinces of Vietnam. The life cycle extends over two years (721.7 days ± 17.6 days), with overwintering as larvae. Adults emerge and disperse in summer. Females lay 6–12 eggs during an oviposition period of 2–3 days, and the incubation period ranges from 27 to 38 days. The larval and pupal periods take 554–701 days and 40–59 days, respectively. Adults survive for 12–23 days. In 2019, the damage incidence (P%) and the damage index (DI) in Yen Bai, Lao Cai, Lai Chau, and Dien Bien provinces ranged from 43.5% to 71.6% and 0.80 to 1.78, respectively. Further research on the distribution and host range of B. medioviolacea is required to help formulate a management strategy for this new orchard pest.
Rethinking Horticulture to Meet Sustainable Development Goals—The Case Study of Novi Sad, Serbia
Authors: Ljubojević Mirjana , Narandžić Tijana , Ostojić Jovana , Božanić Tanjga Biljana , Grubač Milica , Kolarov Radenka , Greksa Amela , Pušić Magdalena
Published: December 2022

With the aim of being a part of global change and providing an example to other researchers throughout the world, this paper details how breeding goals of horticultural plants and their application have shifted in Novi Sad (Serbia) in the last 10–15 years. Contemporary cities/citizens strive to incorporate nature into all of their important life segments and activities, thus requiring an interdisciplinary approach to solving challenges that the 21st century brings. Early research in Novi Sad (Serbia) was focused on the basic genetic, physiological, biochemical and botanical aspects of plant functioning and development in a challenging and changing urban environment abundant in abiotic stressors, as well as biotic and abiotic stressors, that affect the production of horticultural plant in this field. Recently, research interest has shifted towards the sustainable usage of plant genetic resources (roses, autochthonous terrestrial orchids, sweet and sour cherry eco-types, and allochthonous oil-rich species), as well as sustainable practices and nature-based solutions (urban-derived biodiesel, rain gardens, green roofs, green walls, constructed wetlands, water ponds, bioswales and permeable surfaces on a different scale of urban planning). This case study aimed to illustrate how plant selection and breeding strategies can satisfy urban growth demands, whereas urban planning must include sustainable genetic resources suitable for urban ecosystems. Available ornamental plant genetic resources (with pronounced tolerance/resistance to abiotic and/or biotic stressors) associated with the novel approach of their application in green city infrastructure provide the opportunity to implement multiple nature-based solutions leading to numerous ecosystem services. Combined, these contribute to the globally defined goals for sustainable development.
An Integrated Quantitative Risk Assessment Method for Underground Engineering Fires
Authors: Yuan Qi , Zhu Hongqinq , Zhang Xiaolei , Zhang Baozhen , Zhang Xingkai
Published: December 2022

Fires are one of the main disasters in underground engineering. In order to comprehensively describe and evaluate the risk of underground engineering fires, this study proposes a UEF risk assessment method based on EPB-FBN. Firstly, based on the EPB model, the static and dynamic information of the fire, such as the cause, occurrence, hazard, product, consequence, and emergency rescue, was analyzed. An EPB model of underground engineering fires was established, and the EPB model was transformed into a BN structure through the conversion rules. Secondly, a fuzzy number was used to describe the state of UEF variable nodes, and a fuzzy conditional probability table was established to describe the uncertain logical relationship between UEF nodes. In order to make full use of the expert knowledge and empirical data, the probability was divided into intervals, and a triangulated fuzzy number was used to represent the linguistic variables judged by experts. The α-weighted valuation method was used for de-fuzzification, and the exact conditional probability table parameters were obtained. Through fuzzy Bayesian inference, the key risk factors can be identified, the sensitivity value of key factors can be calculated, and the maximum risk chain can be found in the case of known evidence. Finally, the method was applied to the deductive analysis of three scenarios. The results show that the model can provide realistic analysis ideas for fire safety evaluation and emergency management of underground engineering. The proposed EPB risk assessment model provides a new perspective for the analysis of UEF accidents and contributes to the ongoing development of UEF research.
Examining Psychotherapeutic Processes with Depressed Adolescents: A Comparative Study of Two Psychodynamic Therapies
Authors: Calderon Ana , Storeide Knut Arne Hooper , Elvejord Cecilie , Nissen-Lie Helene Amundsen , Ulberg Randi , Dahl Hanne-Sofie Johnsen
Published: December 2022

To understand processes associated with better or poorer psychotherapy outcomes is vital. This study examined and contrasted interaction patterns between one therapist and two depressed 17-year-old girls, Johanna (good outcome) and Sonja (poor outcome), in short-term psychoanalytic therapies selected from an RCT. Outcome data were collected regarding level of inter- and intra-personal functioning and symptoms of depression. Process data were obtained using the Adolescent Psychotherapy Q-Set on all available sessions. Analyses yielded five relational patterns or “interaction structures” in the two therapy processes; Three explained most of the variance in sessions with Johanna (i.e., ‘positive working alliance’, ‘therapist’s active use of psychodynamic techniques’, and ‘a receptive patient’) and two explained more of the variance in sessions with Sonja (i.e., ‘therapist using a more problem-solving and symptom-oriented approach’ and ‘patient displaying limited capacity for mentalization’). The processes in the two cases presented differences related to mentalization, psychological mindedness, and attachment style of the patients. The therapist used different therapeutic approaches, favouring more psychodynamic interventions in the good outcome case and a more problem-solving and symptom-oriented approach with the poor outcome case. In the latter case, the relationship seemed to be more of a struggle.
Novel Insight into Non-Genetic Risk Factors of Graves’ Orbitopathy
Authors: Zawadzka-Starczewska Katarzyna , Stasiak Bartłomiej , Wojciechowska-Durczyńska Katarzyna , Lewiński Andrzej , Stasiak Magdalena
Published: December 2022

An assessment of the risk of Graves’ orbitopathy (GO) is an important challenge in Graves’ disease (GD) management. The purpose of this study was to compare non-genetic parameters in GD patients with and without GO in order to find novel risk factors and to verify the factors already reported. A total number of 161 people, 70 with GO and 91 non-GO patients were included in this study. GO was confirmed to be associated with smoking, older age, higher TSH receptor antibodies (TRAb) and lower thyroglobulin antibody (TgAb) levels and hypercholesterolemia. We demonstrated the latter correlation even for only a mild increase in LDL cholesterol. Importantly, our study provides novel potential GO risk factors, including higher serum creatinine levels, higher MCV and lower PLT. If further confirmed, these new, simple and easily accessible potential GO markers may constitute valuable auxiliary markers in GO risk assessments. We additionally proved that in moderate to severe GO, gender-related differences attenuate. No impact of vitamin D deficiency in GO development in patients with 25-hydroxyvitamin D [25(OH)D] > 20 ng/mL was found. The present report provides a set of GO risk factors, which can be used as a precise tool for an individual GO risk assessment.
Controversy in Electromagnetic Safety
Authors: Chou Chung-Kwang
Published: December 2022

The dramatic increase in electromagnetic fields (EMFs) in the environment has led to public health concerns around the world. Based on over 70 years of research in this field, the World Health Organization (WHO) has concluded that scientific knowledge in this area is now more extensive than for most chemicals and that current evidence does not confirm the existence of any health consequences from exposure to low-level electromagnetic fields. However, controversy on electromagnetic safety continues. Two international groups, the International Committee on Electromagnetic Safety of the Institute of Electrical and Electronics Engineers (IEEE) and the International Commission on Non-Ionizing Radiation Protection, have been addressing this issue for decades. While the goal of both groups is to provide human exposure limits that protect against established or substantiated adverse health effects, there are groups that advocate more stringent exposure limits, based on possible biological effects. Both biological and engineering complexities make the validity of many EMF studies questionable. Controversies in research, publication, standards, regulations and risk communication concerning electromagnetic safety will be addressed in this article. The WHO is conducting systematic reviews on the RF biological effects literature. If scientists would discuss the safety issues of EMFs based on validated scientific facts and not on unreproducible possible effects and opinions, the controversy would be minimized or resolved.
Public Health Interventions to Address Housing and Mental Health amongst Migrants from Culturally and Linguistically Diverse Backgrounds Living in High-Income Countries: A Scoping Review
Authors: Crawford Gemma , Connor Elizabeth , McCausland Kahlia , Reeves Karina , Blackford Krysten
Published: December 2022

Migrants from culturally and linguistically diverse (CaLD) backgrounds experience factors that may increase health inequities related to a range of determinants of health including housing and mental health. However, the intersection between mental health and housing for migrants is poorly understood. A scoping review searched four academic databases for concepts related to cultural and linguistic diversity, housing conditions, and public health interventions to address homelessness. A total of 49 articles were included and seven key themes identified: housing provision; mental health intersections and interventions; complexity and needs beyond housing; substance use; service provider and policy issues; the role of cultural and linguistic diversity; and consumer experience. The intersection of ethnicity with other social determinants of health and housing was highlighted though there were limited interventions tailored for migrants. Studies generally pointed to the positive impacts of Housing First. Other sub-themes emerged: social connection and community; shame, stigma, and discrimination; health and support requirements; and employment, financial assistance, and income. Consumer choice was identified as vital, along with the need for systemic anti-racism work and interventions. To support secure housing for migrants and mitigate mental health impacts, closer attention is required towards migration factors along with broader, tailored services complementing housing provision.
Developing and Validating an Individual-Level Deprivation Index for Children’s Health in France
Authors: Laporte Remi , Babe Philippe , Jouve Elisabeth , Daguzan Alexandre , Mazoue Franck , Minodier Philippe , Noel Guilhem , Urbina Diego , Gentile Stephanie
Published: December 2022

Background: Deprivation generates many health inequalities. This has to be taken in account to enhance appropriate access to care. This study aimed to develop and validate a pediatric individual-level index measuring deprivation, usable in clinical practice and in public health. Methods: The French Individual Child Deprivation Index (FrenChILD-Index) was designed in four phases: item generation then reduction using the literature review and expert opinions, and index derivation then validation using a cross-sectional study in two emergency departments. During these last two phases, concordance with a blinded evaluation by an expert enabled us to determine thresholds for two levels of moderate and severe deprivation. Results: The generation and reduction phases retained 13 items. These were administered to 986 children for the derivation and validation phases. In the validation phase, the final 12 items of the FrenChILD-Index showed for moderate deprivation (requiring single specific care for deprived children) a sensitivity of 96.0% [92.6; 98.7] and specificity of 68.3% [65.2; 71.4]. For severe deprivation (requiring a multidisciplinary level of care), the sensitivity was 96.3% [92.7; 100] and specificity was 91.1% [89.2; 92.9]. Conclusions: The FrenChILD-Index is the first pediatric individual-level index of deprivation validated in Europe. It enables clinical practice to address the social determinants of health and meet public health goals.
Student Presenteeism in Digital Times—A Mixed Methods Approach
Authors: Komp Rebecca , Kauffeld Simone , Ianiro-Dahm Patrizia
Published: December 2022

In young adulthood, important foundations are laid for health later in life. Hence, more attention should be paid to the health measures concerning students. A research field that is relevant to health but hitherto somewhat neglected in the student context is the phenomenon of presenteeism. Presenteeism refers to working despite illness and is associated with negative health and work-related effects. The study attempts to bridge the research gap regarding students and examines the effects of and reasons for this behavior. The consequences of digital learning on presenteeism behavior are moreover considered. A student survey (N = 1036) and qualitative interviews (N = 11) were conducted. The results of the quantitative study show significant negative relationships between presenteeism and health status, well-being, and ability to study. An increased experience of stress and a low level of detachment as characteristics of digital learning also show significant relationships with presenteeism. The qualitative interviews highlighted the aspect of not wanting to miss anything as the most important reason for presenteeism. The results provide useful insights for developing countermeasures to be easily integrated into university life, such as establishing fixed learning partners or the use of additional digital learning material.
Wildlife Undergrads Spread Their Wings in Citizen Science Research Experience
Authors: Ortiz Janel L. , Torres Conkey April A. , Brennan Leonard A. , Fedynich LaVonne , Green Marybeth
Published: December 2022

Citizen science has become a valuable tool for natural resource professionals; however, many undergraduate students are not aware of its use as a means of collecting data for scientific analysis. To address this, we introduced a bird-focused research experience into an undergraduate Wildlife Management Techniques course. The objective of this course is to provide practical experiences in wildlife science by learning and using tools and techniques applied in the field. Students designed, implemented, and presented the results of a wild bird observation survey that contributed data to an existing e-Bird-based program. Pre-post surveys were collected to assess student learning and behavioral changes. We observed increased student awareness of citizen science. Ninety-two percent of students correctly defined citizen science following the experience. However, only sixteen percent of students stated they would continue participation in STWB, fifty percent were unsure, and thirty-four percent would not continue involvement. Improvements are discussed to promote participation in citizen science, connections with the community, and communication skill development for future employment.
Iron Deficiency and Deranged Myocardial Energetics in Heart Failure
Authors: Tkaczyszyn Michał , Górniak Krzysztof Michał , Lis Weronika Hanna , Ponikowski Piotr , Jankowska Ewa Anita
Published: December 2022

Among different pathomechanisms involved in the development of heart failure, adverse metabolic myocardial remodeling closely related to ineffective energy production, constitutes the fundamental feature of the disease and translates into further progression of both cardiac dysfunction and maladaptations occurring within other organs. Being the component of key enzymatic machineries, iron plays a vital role in energy generation and utilization, hence the interest in whether, by correcting systemic and/or cellular deficiency of this micronutrient, we can influence the energetic efficiency of tissues, including the heart. In this review we summarize current knowledge on disturbed energy metabolism in failing hearts as well as we analyze experimental evidence linking iron deficiency with deranged myocardial energetics.
Dietary Behaviors, Serum 25(OH)D Levels and Quality of Life in Women with Osteoporotic Disorders
Authors: Godala Małgorzata , Sewerynek Ewa , Gaszyńska Ewelina
Published: December 2022

Data obtained in recent years clearly demonstrate the aging process of European populations. Consequently, the incidence of osteoporosis has been rising. The aim of this study is to assess the quality of life (QoL) of women with osteoporosis. A total of 260 women participated in this study. The patient group consisted of 170 women with osteoporotic disorders. The control group consisted of 90 healthy women. Participants’ quality of life was measured with the Qualeffo-41 Questionnaire. The total 25(OH)D concentration level was assessed with an assay using the chemiluminescent immunoassay. To assess the pain level, the Visual Analogue Scale (VAS) was used. To assess dietary behaviors, data were obtained by a 13-item Food Frequency Questionnaire. To assess the nutrition knowledge of participants, the Beliefs and Eating Habits Questionnaire was used. Based on the frequency of food intake, participants were classified into three patterns of behavior, i.e., Prudent, Western, and Not Prudent-Not Western. The patients assessed their quality of life as average (36.6 ± 19.9 points). The most favorable scores were obtained in the domains of “Ability to do jobs around the house” and “Mobility”. The worst rated domain among the respondents was “Mental function”. There were significant differences identified in quality of life depending on diet, nutritional knowledge, comorbidities and occurrence of fractures in the subjects. The individuals in the “Prudent” group reported a significantly higher quality of life as compared to the “Not Prudent-Not Western” and “Western” groups and those with high nutritional knowledge as compared to those with moderate and low. Lower quality of life was also observed among women with comorbidities and with bone fractures. Depending on serum 25(OH)D levels, poorer quality of life was characterized women with vitamin D deficiency. Patient education, implementation of effective methods aimed at alleviating pain and maintaining the optimal concentration of vitamin D can help improve the quality of life in patients with osteoporotic disorders.
Theoretical Basis for a Group Intervention Aimed at Preventing High School Dropout: The Case of ‘Guttas Campus’
Authors: Ramsdal Gro Hilde , Wynn Rolf
Published: December 2022

School dropout may have important negative consequences for the individual as well as for society. It is therefore important to help students stay in school. Group interventions have been developed to reduce dropout, but the theoretical underpinnings of such programs are not always obvious. This study focuses on the Norwegian dropout-prevention program named ‘Guttas Campus’ (The Boys’ Camp). We draw on published and unpublished research, other sources of written information, discussions with stakeholders, and direct observation of the intervention, to identify central theories that form the basis of the intervention. These theories are briefly presented, and the impact of the ideas on the intervention is analysed.