bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISANDO AULAS E PRODUZINDO REFLEXÕES SOBRE O ENSINO DE LÍNGUA INGLESA NO COLÉGIO ESTADUAL JARDIM PAULISTA DURANTE A RESIDÊNCIA PEDAGÓGICA
Authors: Vieira Miliane Moreira Cardoso , Lourdes da Silva Santos Beatriz , Sousa Araújo Ana Maria , Martins Aguiar Monteiro Jaiara
Published: April 2020

No presente artigo, Analisando diários de aulas e produzindo narrativas sobre a disciplina de Língua Inglesa nas Escolas durante a Residência Pedagógica, será abordado sobre a prática em sala de aula nas aulas de língua inglesa nas turmas de ensino fundamental 7º ano, 8ºano e 3º ano de Ensino Médio, no Colégio Estadual Jardim Paulista (CEJAP) de Araguaína-To.
O PROGRAMA RESIDÊNCIA PEDAGÓGICA DA UFT/ARRAIAS E OS DESAFIOS DAS ESCOLAS DO CAMPO
Authors: De Jesus Soares Pereira Aparecida , Pereira da Silva Waldir , Ferreira da Cunha Mirian , Fernandes da Conceição Viviane
Published: April 2020

O período do residência pedagógica é na verdade uma reorganização do pensar e das reflexões adquiridas ao longo do Curso de Licenciatura em Educação do Campo – Códigos e Linguagens Artes Visuais e Música da Universidade Federal do Tocantins- Campus de Arraias em relação à prática educativa em sala de aula. Assim, propõe-se com este trabalho fazer um relato das experiências adquiridas no Programa.
SEGURANÇA DOS ALIMENTOS NO CONTEXTO DA PANDEMIA POR SARS-COV-2
Authors: Matos Silva Sousa Hermanny , Ibiapina Andréia , Ribeiro Lima Adriene , Aparecida de Souza Martins Glêndara
Published: April 2020

O mundo está enfrentando uma pandemia que teve como epicentro a cidade de Wuhan, na China, e se espalhou pelo mundo todo. Vários países estão em estado de alerta por se tratar de uma pandemia causada por um vírus com causas e consequências ainda desconhecidas. O vírus é o SARS-CoV2, da família Coronaviridae, onde o SARS-CoV, já ocasionou uma pandemia em 2003, deixando mais de 700 mortos ao redor do mundo. O novo coronavírus, causador da doença denominada COVID-19, afeta principalmente o sistema respiratório e tem uma taxa de transmissão muito alta. Alguns pacientes podem ser assintomáticos, culminando numa alta capacidade de transmissão. Dados científicos comprovam que esse vírus permanece em atividade durante horas, em alguns materiais, trazendo preocupação com a segurança dos alimentos e o questionamento quanto ao contágio através da alimentação. Por isso, é necessário o cuidado na manutenção e higienização das instalações, equipamentos e utensílios, atendendo às Boas Práticas para garantir a segurança sanitária dos alimentos.
Analysis and Forecasting of Viral Hepatitis A Morbidity in the Russian Federation Using the Wald’s Schedule
Authors: Burgasova O. A. , Sayapina L. V. , Volkova V. M. , Postupailo V. B. , Chulanov V. P.
Published: April 2020

Objective of the study was to conduct the analysis and develop the method of forecasting of viral hepatitis A (VHA) incidence using Wald’s schedule. Materials and methods. The work is based on official statistical data of the Rospotrebnadzor on the VHA morbidity rates in the Russian Federation and Moscow city between 2010 and 2016. Results and discussion. It is established that in the overall incidence of VHA cases in the Russian Federation over the period of 2010–2016, 67.7 % were registered among adults and 32.3 % – among children; as for the incidence among adults in Moscow, it accounted for 79.8 %, and for children – 20.2 %. To assess epidemiological situation on VHA, forecasting approach using Wald’s schedule was put forward. Based on the results of the analysis conducted, the threshold values for morbidity rates among adult population in Moscow stood at 38 cases, fluctuations in mean values ranged from 48 to 63 cases. It is shown that the total minimum and maximum levels of morbidity among adult population in 2017 would account for 180 and 624 cases, respectively. Forecast of incidence among children is determined on an accrual basis: minimum monthly level – 7 cases, maximum – 17. Monthly growth of infection is 0.9 cases. It is revealed that possible cumulative minimum and maximum morbidity rates among children would amount to 84 and 204 cases in 2017, respectively. The proposed method of Wald’s schedule for VHA incidence forecasting will allow for determining both monthly minimum and maximum rates of infection for the upcoming period and provide for timely planning of anti-epidemic measures.
Investigation of Live-Stock Animals Seroprevalence to West Nile Virus in the Territory of the Saratov Region
Authors: Kazorina E. V. , Krasovskaya T. Yu. , Kazantsev A. V. , Shcherbakova S. A. , Chastov A. A. , Kutyrev V. V.
Published: April 2020

Aim – to determine the intensity of West Nile virus circulation in anthropogenic biotopes in the territory of the Saratov Region between 2012 and 2017. Materials and methods. 1454 blood serum samples from animals of private households and agricultural cooperatives in 14 districts of the Region were investigated: 1111 samples from horses and 343 samples from the cattle. Sera were examined for the presence of total antibodies to WNV using a test system registered in the Russian Federation to detect antibodies to this virus by a competitive enzyme immunoassay «ID Screen West Nile Competition Multi-species» («ID. Vet», France). Results and discussion. The share of resistant to WNV cohort of live-stock animals in the territory of the Saratov Region over the whole period of study was (14.0±0.9) %, particularly in horses – (15.9±1.1) %, and in the cattle – (7.9±1.5) %. The highest values were observed for horses. High level of resistance in live-stock animals was registered in the districts located on the left bank of the river Volga: central ones – Engels (36.4±5.5), Fedorovsky (29.0±4.6), and Sovetsky (20.0±3.7); southern districts – Rovensky (74.3±5.3 %) and Krasnokutsky (15.7±4.4), which confirms the assumption about the initial introduction of the virus from neighboring territories of the Volgograd Region into the territory of the Left Bank where there are characteristic near-water biotopes and active circulation of the agent takes place. The data obtained on the detection of the resistant live-stock animal cohort testify to the spread of West Nile virus in the territory of the surveyed districts of the Saratov Region and, in particular, anthropogenic biotopes which further points to the formation of persistent natural and natural-anthropourgic foci of West Nile fever.
Efisiensi Teknis Pengelolaan Tanaman Terpadu Padi Sawah di Kabupaten Karanganyar, Provinsi Jawa Tengah
Authors: Novitaningrum Restie , Supardi Suprapti , Marwanti Sri
Published: April 2020

English
A way to increase rice productivity and production is to increase efficiency by technological innovation. Integrated Crop Management (ICM) is an approach to improve the efficiency of rice farming by integration of technological components that are applied according to local specific condition. This study aims to analyze technical efficiency and inefficiency of integrated crop management implementation in rice farming. The study was conducted in Jaten Subdistrict, Karanganyar Regency, Central Java Province, in 2017. Primary data was collected from samples of 51 farmers that implemented the ICM and 42 farmers that did not use ICM. Data were analyzed using stochastic frontier production function that was estimated with the Maximum Likelihood Estimation method. The results show that rice farms have been technically efficient in general. The technical efficiency of ICM adopters in the range of 71,41-98,14% with average 94,04%, higher than those of non ICM adopters in the range of 68,50-96,96% with average 91,72%. There is a room for increasing efficiency. Formal education and ICM adoption increase technical efficiency. Farmer's ability to implement PTT can be improved through extension and training programs.

Indonesian
Salah satu cara untuk meningkatkan produktivitas dan produksi padi adalah dengan meningkatkan efisiensi melalui inovasi teknologi. Pengelolaan Tanaman Terpadu (PTT) merupakan sebuah pendekatan untuk meningkatkan efisiensi usaha tani padi dengan integrasi komponen-komponen teknologi yang diterapkan sesuai spesifik lokasi. Penelitian ini bertujuan untuk menganalisis efisiensi dan sumber inefisiensi teknis pada penerapan PTT usaha tani padi sawah. Penelitian dilakukan di Kecamatan Jaten, Kabupaten Karanganyar, Jawa Tengah, pada 2017. Data primer dikumpulkan dari 51 petani yang menerapkan PTT dan 42 petani yang tidak menerapkan PTT. Analisis data menggunakan fungsi produksi stokastik frontier yang diduga dengan metode Maximum Likelihood Estimation (MLE). Hasil analisis menunjukkan bahwa usaha tani padi sawah umumnya sudah efisien secara teknis.  Nilai efisiensi teknis usaha tani dengan penerapan PTT berkisar 71,41-98,14% dengan rata-rata 94,04%, lebih tinggi daripada usaha tani tanpa penerapan PTT yang formal berkisar 68,50-96,96% dengan rata-rata 91,72%. Masih ada peluang untuk meningkatkan efisiensi teknis. Pendidikan dan penerapan PTT berpengaruh positif terhadap efisiensi teknis. Kemampuan petani dalam menerapkan PTT dapat ditingkatkan melalui program penyuluhan dan pelatihan.
Effect of Good Corporate Governance on Profitability
Authors: Subarnas Devina , Gunawan Yuliana
Published: April 2020

The research aims to decide the effect of good corporate governance on profitability in banking companies listed on Indonesia stock exchange from 2016 to 2017. This researchwas an explanatory research, using secondary data. The sample was selected using the purposive sampling method, which resulted in a total of 28 sample companies. The data analysis used was multiple linear regression. The results show that the board of directors significantly affect profitability and independent commissioners does not significantly affect profitability. Simultaneously, board of directors and independent commissioners significantly affect profitability.
Experiencia pedagógica para la enseñanza de la biomecánica mediada por software en escuelas de ingeniería biomédica
Authors: Martín-Perico Juana Yadira , Garibello-Suan Bernardo
Published: April 2020

El presente documento contiene la descripción de una experiencia de aula desarrollada en una escuela de ingeniería biomédica de Bogotá, cuya estrategia fue emplear el software de análisis de movimiento como mediación pedagógica a fin de articular al estudiante en el marco de la sociedad del conocimiento y sus recursos, lo que lo llevó a contrastar los conceptos de biomecánica con lo observado en la tecnología y así estar en capacidad de caracterizar el cuerpo humano en aplicaciones de clínica, deporte e investigación. El ejercicio didáctico propendió a minimizar la reprobación académica en el área; incrementó la capacidad descriptiva, de síntesis y de argumentación del estudiante; permitió que los discentes propusieran ideas para sus proyectos de grado y brindó la oportunidad a los docentes de articular lo trabajado en el aula con la práctica de laboratorio a fin de trabajar proyectos transdisciplinares. La contribución del software en el análisis de movimiento en situaciones biomecánicas patológicas es una oportunidad para que el estudiante comprenda las condiciones de adaptación del cuerpo humano, lo cual conduce al desarrollo de habilidades especiales que son de gran interés para proponer diseños de ayudas funcionales y la creación de dispositivos biomédicos para contribuir de esta manera en la mejoría de la calidad de vida de las personas a través del incremento de los desarrollos de ingeniería biomédica en campos como las ayudas funcionales, los dispositivos de rehabilitación y la protección industrial.
A Surgical Reduction Technique for Posterior Cruciate Ligament Avulsion Fracture in Total Knee Arthroplasty: A Comparison Study
Authors: Lin Wei , Niu Jinghui , Dai Yike , Zhang Huaxing , Zhu Jing , Wang Fei
Published: April 2020

Background: Posterior cruciate ligament (PCL) avulsion fracture of tibia is an uncommon but serious complication during primary cruciate retaining total knee arthroplasty (TKA). The first objective of this report was to conduct a retrospective cohort study to investigate the incidence and potential risk factors of PCL avulsion fracture in primary cruciate-retaining TKA. The second objective was to assess the functional outcomes of the knee after reduction of PCL avulsion fracture.Methods: From January 2014 to January 2016, 56 patients who experienced PCL avulsion fracture of tibia in primary cruciate-retaining TKA were included in study group. Patients in this group underwent reduction of avulsion fracture. In this period, we selected 224 patients (control group) for comparison. Patients in this group also underwent the same TKA but no PCL avulsion fracture occurred. Range of motion of the knee and Knee Society Scores were assessed. The forgotten joint score was used to analyze the ability to forget the joint. Differences were considered statistically significant at p < 0.05.Results: In our series, the incidence of PCL avulsion fracture was 4.6%. There was no significant differences (p > 0.05) with regard to preoperative or postoperative range of motion of the knee, final 4 year mean clinical score in study and control groups 92.4 ± 2.7 and 93.6 ± 1.9, respectively, and mean functional scores of 85.1 ± 1.8 and 87.1 ± 1.2, respectively.Conclusions: The incidence of PCL avulsion fracture of tibia is relatively high. Older age and female gender were two risk factors of fracture in primary cruciate-retaining TKA. Reduction of PCL avulsion fracture with high-strength line can achieve good stability and function of the knee.
Mentored training and its association with dissemination and implementation research output: a quasi-experimental evaluation
Authors: Jacob Rebekah R , Gacad Angeline , Padek Margaret , Colditz Graham A , Emmons Karen M , Kerner Jon F , Chambers David A , Brownson Ross C
Published: April 2020

Background There is continued need to evaluate training programs in dissemination and implementation (D&I) research. Scientific products yielded from trainees are an important and objective measure to understand capacity growth within the D&I field. This study evaluates our mentored training program in terms of scientific productivity among applicants.Methods Post-doctoral and early career cancer researchers were recruited and applied to the R25 Mentored Training for Dissemination and Implementation Research in Cancer (MT-DIRC) between 2014 and 2017. Using application details and publicly available bibliometric and funding data, we compared selected fellows with unsuccessful applicants (nonfellows). We extracted Scopus citations and US federal grant funding records for all applicants (N=102). Funding and publication abstracts were de-identified and coded for D&I focus and aggregated to the applicant level for analysis. Logistic regression models were explored separately for the odds of 1) a D&I publication and 2) US federal grant funding post year of application among fellows (N=55) and nonfellows (N=47). Additional models were constructed including independent variables that attenuated the program’s association by 5 percent or more. Only US-based applicants (N=87) were included in grant funding analysis.Results Fellows and nonfellows were similar across several demographic characteristics. Fellows were more than 3 times more likely than nonfellows to have grant funding after MT-DIRC application year (OR 3.2; 95% CI: 1.1-11.0) while controlling for time since application year; the association estimate was 3.1 (95% CI: 0.98-11.0) after adjusting for both cancer research area and previous grant funding. For publications, fellows were almost 4 times more likely to publish D&I focused work adjusting for time (OR 3.8; 95% CI: 1.7-9.0). This association lessened after adjusting for previous D&I publication and years since undergraduate degree (OR 2.9; 95% CI: 1.2-7.5).Conclusions We document the association of a mentored training approach with built-in networks of peers to yield productive D&I researchers. Future evaluation efforts could be expanded to include other terms of longer-term productivity such as policy or practice change as additional objective measures. D&I research trainings in the US and internationally should consider common evaluation measures.
Simulation of tennis serve behavior based on video image processing and wireless sensor technology
Authors: Li Xiao-zhong , Huang Ping-lin
Published: April 2020

Video-based human motion analysis is an important research direction in the field of computer vision. It detects moving objects from video sequences, extracts key parts of the human body, and obtains useful information for human movements. Further analysis and identification. In this paper, the joints of the teeing arm are first color-coded. The tennis teeing video is collected by a high-speed camera. The coordinates of the tacking points in each frame are used instead of the knuckles to study the trajectory of the teeing arm. In the process of video processing, after constructing a dictionary for a series of noise maps, the sparse representation idea was used to reconstruct an interference-free service diagram, and a mixture of Gaussian background modeling was used to extract the foreground of the motion. After obtaining the motion foreground, the marker points are extracted through the color features, and binarization operations are performed on the marker points. Next, the outline of the marker points is searched, the outline is surrounded by the minimum circle, and the returned circle center coordinates are used as the joint point coordinates. Taking the trajectory of the shoulder marking point as a research object, a tennis serving model based on an improved support vector machine was established.
Evaluation of the Efficacy and Safety of Intravenous Remdesivir in Adult Patients with Severe Pneumonia caused by COVID-19 virus Infection: study protocol for a Phase 3 Randomized, Double-blind, Placebo-controlled, Multicentre trial
Authors: Horby Peter W , Cao Bin , Wang Yeming , Wang Chen
Published: April 2020

Background: A novel coronavirus emerged in Wuhan, Hubei Province, China towards the end of 2019 (SARS-CoV-2 or COVID-19 virus). Large scale spread within China and internationally led the World Health Organisation to declare a Public Health Emergency of International Concern on 30th January 2020. The clinical manifestations of COVID-19 virus infection include asymptomatic infection, mild upper respiratory symptoms, severe viral pneumonia with respiratory failure and even death. There are no antivirals of proven clinical efficacy in coronavirus infections. Remdesivir (GS-5734), a nucleoside analogue, has inhibitory effects on animal and human highly pathogenic coronaviruses, including MERS-CoV and SARS-CoV, in in-vitro and in-vivo experiments. It is also inhibitory against the COVID-19 virus in-vitro. The aim of this study is to assess the efficacy and safety of remdesivir in adult patients with severe pneumonia caused by COVID-19 virus infection.Methods: The protocol is prepared in accordance with the SPIRIT (Standard Protocol Items: Recommendations for Interventional Trials) guidelines. This is a phase 3, randomized, double-blind, placebo-controlled, multicentre trial. Adults (≥18 years) with laboratory confirmed COVID-19 virus infection, and severe pneumonia signs or symptoms, and radiologically confirmed severe pneumonia are randomly assigned in a 2:1 ratio to intravenous remdesivir or placebo for 10 days. The primary endpoint is time to clinical improvement (censored at Day 28), defined as the time (in days) from randomization of study treatment (remdesivir or placebo) until a decline of two categories on a six-category ordinal scale of clinical status (1 ꞊ discharged; 6 ꞊ death) or live discharge from hospital. One interim analysis for efficacy and futility will be conducted once half of the total number of events required had been observed.Discussion: This is the first randomized, placebo-controlled trial in 2019-nCoV. Enrolment began in sites in Wuhan, Hubei Province, China on 6th February 2020.Trial registration: ClinicalTrials.gov, NCT04257656, 6th February 2020.
Identification and genetic analysis of qCL1.2 , a novel allele of the “green revolution” gene SD1 from wild rice ( Oryza rufipogon ) that enhances plant height
Authors: Zhang Lizhen , Huang Jingfen , Wang Yanyan , Xu Rui , Yang Ziyi , Zhao Zigang , Liu Shijia , Tian Yunlu , Zheng Xiaoming , Li Fei , Wang Junrui , Song Yue , Li Jiaqi , Cui Yongxia , Zhang Lifang , Cheng Yunlian , Lan Jinhao , qiao weihua , Yang Qingwen
Published: April 2020

Background: The exploitation of novel alleles from wild rice that were lost during rice cultivation could be very important for rice breeding and evolutionary studies. Plant height (PH) was a target of artificial selection during rice domestication and is still a target of modern breeding. The “green revolution” gene semi-dwarf 1 (SD1) were well documented and used in the past decades, allele from wild rice could provide new insights into the functions and evolution of this gene.Results: We identified a PH-related quantitative trait locus, qCL1.2, from wild rice using a set of chromosome segment substitution lines. qCL1.2 encodes a novel allele of SD1 gene. The wild allele of SD1 is a dominant locus that can significantly promote rice internode length by regulating the expression levels of genes involved in gibberellin biosynthesis and signal transduction. Nucleotide diversity and haplotype network analyses of the SD1 gene were performed using 2,822 rice landraces. Two previously reported functional nucleotide polymorphisms clearly differentiated japonica and indica rice; however, they were not associated with PH selection. Other new functional nucleotide polymorphisms in the coding, but not promoter, regions were involved in PH selection during rice domestication. Our study increases understanding of the rice SD1 gene and provides additional evidence of this gene’s selection during rice domestication.Conclusions: Our findings provide evidence that SD1 gene from wild rice enhances plant height and new functional nucleotide polymorphisms of this gene were artificially selected during cultivated rice differentiation.
Improvement in Circulating Endothelial Progenitor Cells Pool After Cardiac Resynchronization Therapy: Increasing the List of Benefits
Authors: Cristóvão Gonçalo , Milner James , Sousa Pedro , Ventura Miguel , Cristóvão João , Elvas Luís , Paiva Artur , Gonçalves Lino , Ribeiro Carlos Fontes , António Natália
Published: April 2020

Background Recent studies suggest that circulating endothelial progenitor cells (EPCs) may influence the response to cardiac resynchronization therapy (CRT). The aim of this study was to evaluate the effect of CRT on EPCs levels and to assess the impact of EPCs on long-term clinical outcomes. Population and methods Prospective study of 50 patients submitted to CRT. Two populations of circulating EPCs were quantified previously to CRT implantation: CD34 + KDR + and CD133 + KDR + cells. EPCs levels were reassessed 6 months after CRT. Endpoints during the long-term follow-up were all-cause mortality, heart transplantation and hospitalization for heart failure (HF) management. Results The proportion of non-responders to CRT was 42% and tended to be higher in patients with an ischemic vs non-ischemic etiology (64% vs 35%, p=0.098). Patients with ischemic cardiomyopathy (ICM) showed significantly lower CD34+KDR+ EPCs levels when compared to non-ischemic dilated cardiomyopathy patients (DCM) (0.0010 ± 0.0007 vs 0.0030 ± 0.0024 cells/100 leukocytes, p=0.032). There were no significant differences in baseline EPCs levels between survivors and non-survivors nor between patients who were rehospitalized for HF management during follow-up or not. At 6-month follow-up, circulating EPCs levels were significantly higher than baseline levels (0.0024 ± 0.0023 vs 0.0047 ± 0.0041 CD34 + KDR + cells/100 leukocytes, p=0.010 and 0.0007 ± 0.0004 vs 0.0016 vs 0.0013 CD133 + /KDR + cells/100 leukocytes, p=0.007). Conclusions Patients with ICM showed significantly lower levels of circulating EPCs when compared to their counterparts. CRT seems to improve the pool of endogenously circulating EPCs and reduced baseline EPCs levels seem not influence long-term outcomes after CRT.
Socio-demographic Determinants of Low Sexual Desire and Hypoactive Sexual Desire Disorder: A Population-based Study in Iran
Authors: Hamzehgardeshi Zeinab , Malary Mina , Moosazadeh Mahmood , Khani Soghra , Pourasghar Mehdi
Published: April 2020

Background: Various socio-demographic factors are determinants of Low Sexual Desire (LSD), but whether these are the determinants of Hypoactive Sexual Desire Disorder (HSDD) are unclear. The aim of this study is to evaluate the Socio-demographic determinants of LSD and HSDD in Iranian women of reproductive age.Methods: This was a population-based, cross-sectional study of 1000 Iranian women of reproductive age (15-49 years) who met the inclusion criteria and were chosen through systematic random sampling from all the healthcare centers in Sari, Iran. LSD was defined as a score no higher than 33 on the Sexual Interest and Desire Inventory-Female (SIDI-F); sexually related personal distress was defined as a score of at least 11.0 on the Female Sexual Distress Scale–Revised (FSDS-R); and HSDD was defined as a combination of these scores. Descriptive statistics were used to describe demographic characteristics while for analyzing grouped variables, chi-square test was applied. Multivariate regression test was also used to adjust the effect of confounding variables. Results: The mean score of sexual interest/desire among women who referred to healthcare centers in the city of Sari is estimated as 30.6±10.5. After adjusting the effect of the confounder variables by logistic regression multivariate analysis, the age at first intercourse, the length of time spent in marriage, and the level of satisfaction with income were variables significantly associated with LSD and HSDD (P < .01). Although increasing individual′s age (P < .001) and body mass index (P < .01) were predictors of LSD in women, HSDD was not statistically significant.Conclusion: There are some factors that are associated with LSD in women but are not related to HSDD. In other words, some factors associated with LSD do not cause personal distress which are one of the criteria necessary for HSDD.
Alleviating psychological distress associated with a positive cervical cancer screening result: a randomized control trial
Authors: Isaka Yukari , Hori Ai , Tanaka Rie , Ichikawa Masao
Published: April 2020

Background: The method of communicating a positive cancer screening result should seek to alleviate psychological distress associated with a positive result. We evaluated whether the provision of information through a leaflet would help reduce psychological distress.Methods: This study design was a simple randomized controlled trial that randomly assigned individual participants to the intervention and control groups and measured outcomes before and after the intervention. The analysis included 972 women. Women received hypothetical cervical cancer screening results, with a leaflet (intervention group) or without it (control group), randomly. Outcomes were psychological distress and intention to undergo further examination.Results: After the intervention, psychological distress appeared to be higher in the control group than in the intervention group among those who received a hypothetical positive screening result (odds ratio: 2.57, 95% confidence interval: 1.87–3.54), while 95% and 97% of those in the intervention and control groups, respectively, reported that they would undergo further examination.Conclusions: Information provision might help reduce psychological distress but not hinder further examination among women who screen positive for cervical cancer.Trial registration: UMIN Clinical Trials Registry UMIN000029894. Date of Registration: November 2017.
The Impact of Labor Stage Duration on Adverse Maternal and Neonatal Outcomes in Multiparous Women: A Retrospective Cohort Study
Authors: wang li , Wang hongxia , jia lu , qing wenjie , Li fan , Zhou Jie
Published: April 2020

Background The duration of first and second stages of labor was increased in the consensus that American College of Obstetricians and Gynecologists (ACOG) published 2014. Studies showed increased adverse maternal and neonatal outcomes in nulliparous women with prolonged second stage of labor. It is not very clear that the impact of labor stage duration on multiparous women.Methods A retrospective cohort study was performed. Cephalic, term, singleton multiparous women were included, who planned for vaginal delivery. Adverse maternal outcomes were defined as referral cesarean delivery, instrumental delivery, postpartum hemorrhage, perineal laceration (3rd and 4th degree), hospitalization stay ≥ 90%th, and adverse neonatal outcomes as NICU, shoulder dystocia, Apgar score ≤ 7(5 min), neonatal resuscitation, assisted ventilation required immediately after delivery. We defined the total stage included the first and second stage of labor.Results There were 7109 parturients included, The duration of first stage was 6.2(3.6–10.0) hours in multiparous women, the second stage was 0.3(0.2–0.7) hours, the total stage was 6.9(4.1–10.7) hours. In the first stage, the rate of overall adverse outcomes was 21%, 23.4%, 28.8%, 35.5%, 38.4% in < 6 h, 6-11.9 h, 12-17.9 h, 18-23.9 h, ≥ 24 h, increased significantly (X2 = 57.64, P๤0.001). Compared with < 6 h, ARR(95%CI) were 1.10(0.92,1.31), 1.33(1.04,1.70), 1.80(1.21,2.68), 2.57(1.60,4.15);In the second stage, the rate of overall adverse outcomes increased from 20.0%, 30.7%, 38.5%, to 61.2%, 69.6% in < 1 h, 1-1.9 h, 2-2.9 h, 3-3.9 h, ≥ 4 h (X2 = 349.70, P๤0.001). Compared with < 1 h, ARR (95% CI) were 1.89 (1.50, 2.39), 2.22 (1.55, 3.18), 10.64 (6.09, 18.59), 11.75 (6.55, 21.08); In the total stage, the rate of overall adverse outcomes were 21.5%, 30.8%, 42.4% in < 12 h, 12-23.9 h, ≥ 24 h (X2 = 84.90, P๤0.001). Compared with < 12 h, ARR (95% CI) were 1.41(1.16,1.72), 3.17 (2.10,4.80).Conclusions The prolonged labor stage may lead to increased adverse outcomes in multiparous women, even in the first stage. The duration of labor stage was an independent risk factor for adverse maternal and neonatal outcomes.
Intestinal barrier breakdown and mucosal microbiota disturbance in neuromyelitis optical spectrum disorder
Authors: Cui Chunping , Tan Sha , Tao Li , Gong Junli , Chang Yanyu , Wang Yuge , Fan Ping , He Dan , Ruan Yiwen , Qiu Wei
Published: April 2020

Background and purpose
The mechanism underlying the pathology of neuromyelitis optica spectrum disorder (NMOSD) remains unclear even though increased expression of the water channel protein aquaporin-4 (AQP4) on astrocytes plays an important role. Our previous study revealed that dysbiosis was detected in the faecal microbiota of NMOSD patients. In this study, we further investigated whether the intestinal barrier and mucosal flora balance were also interrupted in NMOSD patients.
Methods
Sigmoid mucosal biopsies were collected via endoscopy from six patients with NMOSD and compared with those from three patients with multiple sclerosis (MS) and five healthy controls (HCs). These samples were processed for electron microscopy and immunohistochemistry to investigate changes in ultrastructure and in the number and size of intestinal inflammatory cells. Changes in mucosal flora were also analysed by high-throughput 16S ribosomal RNA gene amplicon sequencing.
Results
The intercellular space between epithelia of the colonic mucosa became wider in MS and NMOSD patients compared to the HCs (P < 0.01), and the expression of tight junction proteins in MS and NMOSD patients significantly decreased compared to that in the HCs. Activation of microphages with many inclusions inside the cytoplasm and enlarged plasmocytes with more particles were found in the NMOSD group. Quantitative analysis showed that the percentage of small-size CD 38 + and CD138 + cells was lower but that of larger-size cells became higher in NMOSD patients, and 16S data showed that the abundance of Streptococcus and Granulicatella was dramatically increased in NMOSD patients.
Conclusions
NMOSD patients exhibited a disrupted intestinal barrier and intestinal dysbiosis and activation of intestinal inflammation, which suggested a potential pathophysiological mechanism of NMOSD underlying intestinal inflammation.
Differentially expressed circRNA and mRNA profiles of neural stem cells with radiation irradiation
Authors: Cai Zhaopeng , Wang Shan , Xie Zhongyu , Wang Peng , Shen Huiyong , Chen Keng
Published: April 2020

Background Spine metastasis is common but highly problematic in clinical oncology practice. Radiotherapy plays an important role in the treatment of spine metastasis, but it at the same time damages the nervous tissue, especially the neural stem cell (NSC), and leads to radiation induced myelopathy. Circular RNA (circRNA) is a kind of non-coding RNA which responses to external stimulus and regulates cellular functions. However, the mechanism of radiotherapy affecting NSC and the role of circRNA in this process are still unclear. Methods The circRNA and mRNA of NSC treated with radiation or not were detect using next-generation sequencing. RT-PCR assays were preformed to confirm the sequencing results and the feature of differentially expressed circRNA. Bioinformation analyses were conducted to identified the critical circRNA and mRNA, as well as the enriched functions and pathways. Moreover, a circRNA-miRNA-mRNA network was constructed to investigate the possible regulatory mechanism. Results A total of 421 differentially expressed circRNA and 1602 differentially expressed mRNA of NSC were identified after radiotherapy. The GO and KEGG analysis of the differentially expressed mRNA as well as the host genes of the differentially expressed circRNA were performed and several key signal pathways such as MAPK signal pathway were identified. Moreover, a circRNA-miRNA-mRNA network focusing on MAPK signal pathway was shown and predicted that chr5:127160496|127165240 could be the critical circRNA in the regulatory mechanism of radiation treated NSCs. Conclusion Our finding showed the differentially expressed circRNA and mRNA profiles of NSC after radiotherapy, suggesting that circRNA may contribute to the pathogenesis of radiation induced myelopathy.
The Value of p16INK4a Immunostaining for High-grade Squamous Intraepithelial Lesions in Human Papillomavirus–Negative Patients
Authors: Zhang Dai , Song Jie , Zhang Xiaosong , Bi Hui
Published: April 2020

Objective This study aims to evaluate the value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus–negative patients in Beijing, China.Methods In this study, we evaluated the value of p16INK4a immunostaining, as well as cytology and colposcopy, for predicting high-grade squamous intraepithelial lesions (HSIL) in human papillomavirus (HPV)-negative patients by comparing the methods with the haematoxylin and eosin (H&E) staining pathological diagnosis of HPV-negative patients.Results Of 122 patients negative for the high-risk HPV (HR-HPV) subtype, 26 (21.3%) underwent colposcopically directed multiple punch cervical biopsy with a H&E pathological diagnosis of HSIL and above (HSIL+), 11 patients (9.0%) had cervical intraepithelial neoplasia (CIN)2, nine patients (7.4%) had CIN3, and six patients (4.9%) had infiltrating carcinoma. Cytology, colposcopy, and p16INK4a immunostaining had 52.4%, 38.5%, and 92.3% sensitivity, respectively, and 76.2%, 94.8%, and 99% specificity, respectively. The positive predictive value of cytology, colposcopy, and p16INK4a immunostaining was 31.4%, 66.7%, and 96%, respectively, and the negative predictive value was 88.5%, 85.1%, and 97.9%, respectively. Compared with H&E staining, the kappa of cytology, colposcopy, and p16INK4a immunostaining was 0.327, 0.323, and 0.926, respectively.Conclusion Positive p16INK4a immunostaining is very strongly consistent with an H&E diagnosis of CIN2+, and it can be used as an objective detection index for HSIL + diagnosis of HPV-negative patients.
A new diagnostic work-up for defining anemia etiologies: a cohort study in general practices
Authors: Schop Annemarie , Stouten K. , Riedl J.A. , Houten R.J. van , Leening M.J.G. , Rosmalen J. van , Bindels P.J.E. , Levin M-D.
Published: April 2020

Purpose.
To study etiologies of anemia using an extensive laboratory analysis in general practices.
Method.
An extensive laboratory analysis was performed in blood of newly diagnosed anemia patients aged ≥ 50 years from the general population in the city of Dordrecht area, the Netherlands. Eight laboratory-orientated etiologies of anemia were defined. Patients were assigned one or more of these etiologies on the basis of their test results.
Results.
Blood of 4152 patients (median age 75 years; 49% male) was analyzed. The anemia etiology was unclear in 20%; a single etiology was established in 59%; and multiple etiologies in 22% of the patients. The most common etiologies were anemia of chronic disease (ACD) (54.5%), iron deficiency anemia (IDA) (19.1%) and renal anemia (13.8%). The most common single etiologies were IDA (82%) and ACD (68%), while the multiple etiologies most commonly included folic acid deficiency (94%) and suspected bone marrow disease (88%). Older age was associated with a lower incidence of IDA and a higher incidence of renal anemia. Mild anemia was more often associated with ACD and uncertain anemia, while severe anemia was mainly seen in patients with IDA.
Conclusion.
Extensive laboratory analysis in anemic patients from the general population helped clarify the etiology of anemia and revealed many various combinations of etiologies in a significant proportion of patients. Age, sex and the severity of anemia are predictive of the underlying etiology.
Validation of the Arabic Version of the Perinatal Anxiety Screening Scale (PASS) among Antenatal and Postnatal Women
Authors: Jr Hoda , Alfarhan Thikrayat
Published: April 2020

BackgroundAnxiety among women in the perinatal period is common. Assessing the severity of perinatal anxiety will help monitor the progress of the patient through the stages of anxiety and facilitated the treatment. This study assesses the validity and reliability of the “Perinatal Anxiety Screening Scale” (PASS) in the Arabic language.MethodsThe PASS was translated into Arabic. 217 women in the antenatal and postnatal phase participated (92 antenatal and 125 postnatal) answered to PASS, GHQ12, and EPDS. Content validity, factor analysis, internal consistency, and test retest reliability were assessed.ResultsContent Validity Index (CVI) and Content Validity Ratio (CVR) were .88 and 0.79; respectively. The scale loaded on four components: acute anxiety, social anxiety, and dissociation; specific fears and trauma; general anxiety and adjustment; and perfectionism and control. Cronbach’s Alpha value for the scale was 0.78 and test retest correlation coefficient was 0.94. PASS significantly correlated with EPDS (rho = 0.46) and GHQ12(rho = 0.58).ConclusionThe Arabic translated version of the PASS showed reasonably adequate validity and reliability and can be used to screen for anxiety disorder among women in the perinatal phase.
The plasma levels of mCRP is linked to cardiovascular disease in antineutrophil cytoplasmic antibody -associated vasculitis
Authors: Wu Kaili , Liang Qinghui , Ding Na , Li Bowei , Hao Jian
Published: April 2020

Background C-reactive protein (CRP) has 2 natural isomers, C-reactive protein pentamer (pCRP) and C-reactive protein monomer (mCRP). The levels of CRP are significantly elevated in patients with anti-neutrophil cytoplasmic antibody（ANCA)-associated vasculitis (AAV). mCRP not only can cause the activation of endothelial cells, platelets, leukocytes, and complements, but also have a proinflammatory structural subtype that can localise and deposit in inflammatory tissues, so it is an important mediator of a variety of clinical diseases, such as Ischemia/Reis perfusion (I/R) injury, Alzheimer's disease, age-related macular degeneration, and cardiovascular disease. Complications of myocardial infarction occurred（n=4) in 37 AAV patients we collected, so it is speculated that mCRP may be related to cardiovascular disease in patients. Methods Brain natriuretic peptide (BNP) and mCRP in plasma were tested by enzyme-linked immunosorbent assay (ELISA). The Acute ST-segment elevation myocardial infarction（STEMI) diagnosed by Coronary angiography and then calculated the Gensini score. Echocardiography: ejection fraction （EF%)，left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), left ventricular mass index (LVMI). Glomerular filtration rate (eGFR) was calculated based on serum creatinine, age, and gender. Results The plasma levels of mCRP in AAV was significantly higher than that in healthy volunteers (P<0.001). There were 4 STEMI complications in AAV. We compared mCRP and CRP levels with STEMI complication and without STEMI complications in AAV, the plasma levels of mCRP was higher, however the plasma levels of CRP was lower in STEMI. Plasma levels of mCRP correlated with Birmingham vasculitis activity score (BVAS), eGFR, BNP, EF%, LVEDV, LVESV, LVMI ( r =0.404, P =0.013; r =-0.341, P =0.039; r =0.349, P =0.034; r =-0.362, P =0.027; r =0.375, P =0.022; r =0.334, P =0.043; r =0.736, P <0.001) and STEMI complications’ Gensini score (r=0.977, P=0.023) in AAV, however CRP didn’t correlate with BNP, EF%, LVEDV, LVESV, LVMI and Gensini score. Conclusions The plasma levels of mCRP related to cardiovascular diseases in AAV patients.
Prognostic Genes of Breast Cancer Identified by Gene Co-expression Network Analysis
Authors: Tang Jianing , Luo Yongwen , Wu Gaosong
Published: April 2020

Background
Breast cancer is the mostly diagnosed malignance in female worldwide. However, the mechanisms of its pathogenesis remain largely unknown.
Methods
In this study, we used weighted gene co-expression network analysis (WGCNA) to identify novel biomarkers associated with the prognosis of breast cancer. Gene expression profiles were obtained from the Gene Expression Omnibus (GEO) database.
Results
A total of 5 modules were identified via the average linkage hierarchical clustering. And a module significantly with the pathological grade was screened out. 33 genes with high connectivity in the clinically significant module were identified as hub genes. Among them, CASC5 and RAD51 were negatively associated with the overall survival and disease-specific survival. Similar results were observed in the validation dataset. Protein levels of CACS5 and RAD51 were also significantly higher in tumor tissues compared with normal tissues based on the analysis of the Human Protein Atlas. Convincingly, qRT-PCR analysis of breast cancer tissues and matched paracancerous tissue demonstrated that CACS5 and RAD51 were significantly upregulated in in breast cancer compared to paracancerous tissues. Further cell proliferation assay indicated that CACS5 and RAD51 depletion decreased cell proliferation capability.
Conclusion
In conclusion, our findings suggested that CASC5 and RAD51 could serve as biomarkers related to the prognosis of breast cancer and may be helpful for revealing pathogenic mechanism and developing further research.
Chlorogenic acid alleviates sepsis-induced endothelial barrier dysfunction and the underlying mechanism
Authors: Zhang Zisen , Wu Yue , Zhu Yu , Peng Xiao-yong , Xue Ming-ying , Li Tao , Liu Liangming
Published: April 2020

Background

Chlorogenic acid (CGA) has been shown to improve inflammatory cytokines in patients with ulcerative colitis. Vascular endothelial barrier dysfunction is closely related to sepsis. We hypothesize that CGA plays an important role in treating sepsis by protecting the mesenteric endothelial barrier.
Methods

Severe sepsis was established by cecal ligation and puncture (CLP) in SD rats. Immediately after the induction of sepsis, CGA (20 mg/kg) was administered intravenously, and the effects of CGA on mesenteric vascular leakage, hemodynamics, liver and kidney blood flow, and the ultrastructure of the mesenteric endothelial cells were determined. The transendothelial electrical resistance and the Transwell permeability assays were used for observing endothelial barrier function in vascular endothelial cells (VECs). ZO-1 and VE-cadherin expression were observed by immunohistochemistry and western blotting.
Results

FITC-BSA leakage from mesenteric micro-vessels was significantly increased after septic shock, which was significantly improved by CGA. Liver and kidney blood flow were increased by 41.2% and 38.4%, respectively, after CGA administration compared with the septic shock group. Hemodynamic status was significantly decreased after septic shock, and significantly improved by CGA. The 72-h survival rate of septic shock rats in the CGA group (50%) was significantly higher than the septic shock group (6.25%). CGA improved the tight junction opening after septic shock and also significantly up-regulated the expression of ZO-1 and VE-cadherin. CGA also protected endothelial hyperpermeability against lipopolysaccharide-stimulated VEC injury by increasing the expression of ZO-1 and VE-cadherin in vitro.
Conclusion

CGA was beneficial for endothelial barrier function in rats with septic shock, which is the major contribution to CGA with respecting to improving hemodynamic status and organ perfusion. The underlying mechanism involved CGA up-regulation of ZO-1 and VE-cadherin.
Comprehensive assessment of rheumatoid hand using cluster analysis involving parameter analysis of three major hand deformities: a cross-sectional and longitudinal study
Authors: Toyama Shogo , Oda Ryo , Tokunaga Daisaku , Tsuchida Shinji , Kushida Rie , Kawahito Yutaka , Takahashi Kenji
Published: April 2020

Background
Although drug therapy in rheumatoid arthritis has recently improved, treating established rheumatoid hand consisting of three major deformities—thumb deformity, finger deformities, and ulnar drift—remains a challenge. Underlying complex pathophysiology makes it difficult to comprehensively understand these deformities, and comprehensive assessment methods require accumulated skill and long learning curves. We aimed to establish an easier composite method of understanding the pathophysiology using data from our cohort and cluster analysis.
Methods
We established a rheumatoid hand cohort in 2004, and clinically evaluated 134 hands (67 patients). We repeated the evaluations in 2009 and 2015, which provided data for 297 hands and 43 hands for cross-sectional and longitudinal analyses, respectively. Thumb deformities, finger deformities (swan-neck and boutonnière deformity), and ulnar drift were semi-quantified and entered as parameters into a two-step cluster (cross-sectional) analysis. Parameter distributions were considered to clarify each cluster’s characteristics. Next, hands with cluster change over the study period were reviewed to clarify deformity progression (longitudinal analysis). We also performed a stratified analysis between the clusters and the affected period to clarify whether long affected period plays an important role in deformity progression.
Results
We identified seven clusters: cluster 1: mild finger deformities; cluster 2: type 1 thumb deformity; cluster 3: type 2 thumb deformity and severe ulnar drift; cluster 4: type 3 or 4 thumb deformity and low or moderate swan-neck deformity; cluster 5: various thumb deformities and severe boutonnière deformity; cluster 6: type 1 thumb deformity and severe swan-neck deformity; and cluster 7: type 6 thumb deformity. The ulnar drift parameters were equally distributed among the clusters except for cluster 3. Larger cluster numbers generally indicated lower function. At the study endpoint, cluster 1 had changed mainly to cluster 2 or 4, cluster 2 changed to cluster 3, and cluster 7 was considered the final morphology with the lowest hand function. Patients affected for > 30 years had increased risk of rapid disability progression.
Conclusions
Our comprehensive assessment indicated seven deformity patterns and a progressive course in rheumatoid hand. Using patterns may provide rheumatologists with easier information for practical interventions and to determine functional prognosis.