bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Dinamika Atmosfer Pada Kejadian Banjir, Tanah Longsor, dan Angin Kencang Di Semarang dan Demak Tanggal 13 Maret 2024
Authors: Amri Sayful , Giarno Giarno
Published: November 2024

Pada tanggal 13 Maret 2024 terjadi bencana banjir, tanah longsor, dan angin kencang di wilayah Semarang dan Demak. Bencana ini disebabkan oleh cuaca ekstrim berupa hujan ekstrim disertai badai guntur dan angin kencang. Beberapa penelitian sebelumnya yang mengkaji analisis cuaca ekstrim masih belum detail menjelaskan seluruh faktor yang dapat menjadi penyebab cuaca ektrim tersebut. Oleh karena itu, kajian ini menganalisis seluruh faktor global, regional, hingga lokal, serta analisis fisis awan, sehingga dapat bermanfaat untuk operasional peringatan dini cuaca, terutama saat terjadi gangguan cuaca yang sejenis. Penelitian ini menggunakan pendekatan metode analisis deskriptif kuantitatif, terhadap analisis ENSO, DM, MJO, CENS, SST, MSLP, streamline angin gradien, LLMT serta divergensinya, labilitas udara atas, fisis awan, curah hujan dan pasang surut air laut. Hasil penelitian ini menunjukkan bahwa adanya MJO dan gelombang Rossby Ekuator meningkatkan moisture di wilayah Indonesia. Selain itu, adanya bibit siklon tropis 91S di perairan selatan Jawa memicu penguatan aliran monsun Asia dan menyebabkan cross-equatorial flow dan meningkatkan transpor kelembapan dari LCS ke wilayah pesisir Utara Jawa. SST yang hangat, udara atas yang labil, serta konvergensi LLMT di wilayah Semarang dan sekitarnya semakin memicu terbentuknya MCS yang menjadi penyebab hujan ekstrim yang disertai badai guntur dan angin kencang, sehingga memicu terjadinya bencana hidrometeorologi di Semarang dan Demak pada tanggal 13 Maret 2024.
Faktor Penyebab Terjadinya Gastritis pada Remaja Di Puskesmas Deket Kabupaten Lamongan
Authors: Khusna Siti Asmaul , Faridah Virgianti Nur , Lestari Trijati Puspita
Published: November 2024

Banyaknya masyarakat yang menganggap gastritis adalah penyakit ringan tidak berbahaya, sehingga jika tidak segera ditangani dapat bertambah parah dan dapat menurunkan kualitas hidup serta menjadikan hidup kurang produktif. Mencegah penyakit gastritis sebaiknya dengan cara memahami faktor-faktor yang mempengaruhi terjadinya gastritis. Tujuan penelitian ini adalah untuk mengetahui faktor penyebab terjadinya gastritis pada remaja di Puskesmas Deket Kabupaten Lamongan. Jenis penelitian yang digunakan adalah analisis deskriptif dengan pendekatan cross sectional menggunakan simple random sampling di dapatkan 96 responden. Data penelitian ini diambil dengan menggunakan lembar kuesioner. Setelah ditabulasi data akan dianalisis dengan menggunakan uji Spearman Rank (Rho) dengan tingkat kemaknaan p=<0,05. Hasil penelitian menununjukkan 62 responden (64,6%) mengalami gastritis akut, pola makan kurang 71 responden (74%), sering mengkonsumsi kopi 66 responden (68,8%), merokok ringan 60 responden (62,55%), stress normal 67 responden (59,8%). Sedangkan dari hasil uji analisis diperoleh hasil ada hubungan antara pola makan dengan frekuensi kejadian gastritis sebesar (P=0,000), konsumsi kopi (P=0,027), konsumsi rokok (P=0,022),  tingkat stress (P=0,045), yang memiliki kekuatan korelasi hubungan cukup kuat yaitu pada faktor pola makan. pola makan merupakan faktor utama dalam terjadinya kekambuhan penyakit gastritis. Karen secara alami lambung akan terus memproduksi asam lambung setiap waktu dalam jumlah yang kecil setelah 4-6 jam bila seseorang telat makan sampai 2-3 jam, maka asam lambung yang diproduksi dan berlebih dapat mengiritasi mukosa lambung serta menimbulkan rasa nyeri di sekitar epigastrium semakin banyak.
UNDERSTANDING THE CONTINUANCE OF ELECTRONIC PAYMENTS USAGE AFTER COVID-19: A SURVEY IN INDONESIA
Authors: Fadilla Maulyta Noer , Wilantika Nori , Gandhi Arfive
Published: November 2024

During the ongoing pandemic with elevated COVID-19 cases, efforts to minimize direct physical contact for virus prevention have been heightened. Consequently, there has been a strong emphasis on adopting non-cash transactions, particularly electronic payments. As the Indonesian government revoked the social restriction policy on December 30, 2022, people gradually resumed normal activities such as work, school, and shopping. The question arises whether the widespread adoption of electronic payments will persist after COVID-19. To understand this and the factors influencing the sustained use of electronic payments, this study utilized the UTAUT, Trust, and Perceived Security as the research model. The findings indicate that all 920 survey participants maintain their electronic payment usage after COVID-19. Through PLS-SEM analysis, key factors contributing to the sustained use of electronic payment after COVID-19 include the intention to use electronic payments, user trust, performance expectations, facilitating conditions, and perceived security. Additional variables proposed in this research, user trust and perceived security, are proven to have an influence on users' intentions to continue using electronic payments.
CRITICAL SUCCESS FACTORS OF AGILE SOFTWARE DEVELOPMENT IN WATERFALL PROJECT: A CASE STUDY APPROACH
Authors: Bayu Indra , Raharjo Teguh , Syahbuddin Bob Hardian
Published: November 2024

The evolution of software development methodologies has seen Agile rise in response to the limitations of traditional approaches like Waterfall, characterized by its iterative, collaborative, and adaptable nature. However, integrating Agile within the rigid, structured frameworks of organizations accustomed to Waterfall presents significant challenges. This study addresses how to effectively combine these methodologies to mitigate conflicts and achieve successful project outcomes by identifying and analyzing the Critical Success Factors (CSFs) that enable a harmonious integration of Agile into Waterfall environments. Conducted at PT ABC, a firm balancing formal client interactions and contract creation with internal adoption of Scrum, this research uses the Analytic Hierarchy Process (AHP) to systematically prioritize CSFs through literature review, questionnaire development, data collection, and pairwise comparison analysis. The findings reveal that "Communication and Team Environment" is the most influential factor, with a priority vector weight of 0.178, followed by "Project Management and Strategy," "Leadership and Management Support," and "User and Customer Engagement." These factors are pivotal in achieving a balance between control and flexibility in software development projects. The study's implications for PT ABC and other organizations, especially those handling multiple projects and requiring on-site presence while managing other projects, demonstrate how to leverage the strengths of both methodologies for optimal project outcomes. This research provides a model for other organizations striving for similar integrative efforts, showcasing practical strategies to enhance project flexibility and coordination.
SENTIMENT ANALYSIS ON RENEWABLE ENERGY ELECTRIC USING SUPPORT VECTOR MACHINE (SVM) BASED OPTIMIZATION
Authors: Subarkah Pungkas , Kusuma Bagus Adhi , Arsi Primandani
Published: November 2024

Government policy regarding the discourse on the use of renewable energy in electricity, this discourse is widely discussed in the community, especially on social media twitter. The public's response to the implementation of the use of renewable energy varies, there are positive, negative and neutral responses to this government policy. Sentiment analysis is part of Machine Learning which aims to identify responses in the form of text. The data used in this study amounted to 1,367 tweets.  The purpose of this study is to determine the sentiment analysis of government discourse related to the use of renewable energy using an optimisation-based Support Vector Machine (SVM) algorithm approach. This research involves several stages including data collection, data pre-processing, experiments and modelling and evaluation. The data is divided into 3 classes, 120 positive, 1221 neutral and 26 negative. In this research, there are five optimisation models used namely Forward Selection, Backward Elimination, Optimised Selection, Bagging and AdaBoost. The results obtained are the use of Optimised Selection (OS) optimisation with the Support Vector Machine (SVM) algorithm obtained an increase in accuracy from 93% to 96%. The increase in the use of SVM using selection optimization obtained the highest increase, because other optimization techniques only reached 1% and 2% of the original results using the SVM algorithm, namely the accuracy value of 93% to 96% (high accuracy). From the research that has been done, it is certainly important to understand public sentiment towards renewable energy policies, especially renewable energy electricity, the hope is that this research will become a reference for the government.
Pathways to Independence: Family and Individual Dynamics of Leaving Home in Emerging Adulthood
Authors:   , Işık Akın Rengin
Published: November 2024

Despite the increasing trend of young individuals postponing home-leaving over the past decade, leaving home after high school, particularly to attend university, remains a common pathway to independence. Many young individuals still take this opportunity to leave the parental home, and questions regarding the predictors of this significant life course event and its effect at the individual and family level remain important. How do individual and family-related factors influence the likelihood of leaving home in emerging adulthood? To what extent does adolescents’ motivation play a role in the home-leaving process? What is the impact of home-leaving on parent-child relationship quality and well-being? Can we say that leaving home after high school is good or bad for emerging adults’ well-being and their relationship with their parents, or can it be both? The present dissertation aimed to answer these questions by focusing on young individuals’ home-leaving experience starting from the time when they were living with their parents, and by investigating the first potential opportunity for adolescents to leave their parental home: attending university.

Four studies were conducted to address the two overarching aims of the present dissertation. The first aim was to provide insight into the multiple predictors influencing young individuals’ home leaving tendencies as they transitioned from late adolescence to emerging adulthood. The second aim was to investigate changes in parent-child relationship quality and young individuals’ well-being during the transition to university, and to what extent leaving home affected these changes. Given that home-leaving patterns are influenced by country-specific factors, cultural norms, and expectations, this dissertation examined the above-mentioned aims in two distinct cultural contexts: an individualistic (the Netherlands) and a family-oriented culture (Türkiye). Together, the findings of this dissertation offer novel insights into the home-leaving process of young individuals, particularly those attending university. They demonstrate the interplay between family-level and individual-level factors that shape this process in two cultures.
Optimizing Lower Extremity Reconstructions
Authors:   , Krijgh David Dielis
Published: November 2024

This thesis explores strategies to improve the outcomes of patients who need a lower leg reconstruction. Due to the fact that lower extremity reconstructions are multi-facetted, this thesis focuses on three sub-topics:
- What factors impact the outcomes, and how can we improve them?
- How can we provide postoperative care in a more efficient manner? Is dangling necessary?
- What is the prevalence of pain in patients who have had a lower extremity amputation, and what are the therapeutic options?

We found that pain and mental factors have a substantial impact on the outcomes of these patients, and we advocate that mental support should be offered to patients who have had severe lower extremity trauma. The prevalence of pain is underestimated in patients who have had a lower extremity amputation despite the fact that treatment options often still exist. Furthermore, we researched if a dangling protocol after a microvascular tissue transplantation is necessary, and our conclusion is that it is not. Therefore, patients will be able to exit the hospital earlier.
Alat Monitoring Kinerja Pompa Dosing PAC Berbasis IoT (Internet of Things)
Authors: Nurhaliza Susan Siti , Marliza Nana , Prayoga Fengky
Published: November 2024

Abstrak
 
Kemampuan serta perkembangan manusia di bidang teknologi bergerak begitu cepat, seiring berjalanya waktu dari abad ke abad. Berbagai macam teknologi yang manusia ciptakan untuk kepentingan individu maupun kelompok yang memiliki fungsi dan tujuan yang berbagai macam. Salah satunya yaitu Alat Monitoring Kinerja Pompa Dosing PAC Berbasis IoT(Internet of things). Alat monitoring ini tentunya sangat membantu dalam memudahkan operator produksi di instalasi pengolahan air. Tujuannya adalah memonitoring kinerja pompa dan proses koagulasi. Pada saat cairan pac dan air baku melakukan proses pembubuhan pada bak koagulasi yang ada di setiap gedung instalasi, yang nantinya akan menentukan dari hasil air baku yang di olah. Oleh sebab itu, penulis membuat Alat Monitoring Kinerja Pompa Dosing PAC Berbasis IoT(Internet of things), yang akan terhubung ke modul elektronik lainya seperti, colokan adaptor, nodemcu esp8266, modul step down, sensor relay, pompa air mini, sensor hujan, aplikasi blynk, dan device hp samsung A3 2016. Langkah selanjutnya adalah memprogram setiap komponen agar terkoneksi satu sama lainya, oleh nodemcu sebagai basis dari proses yang akan menghasilkan inputan berupa notifikasi kepada user berupa notifikasi pada device.
 
Kata Kunci: Internet Of Thing, Nodemcu, Sensor Relay, Pompa Air, Sensor hujan, Blynk.
 
Abstract
 
Capabilities and development in the field of technology move so fast, over time from century to century. Various kinds of technology that humans have created for the benefit of individuals and groups that have various functions and purposes. One of them is an IOT (Internet of things) based Dosing pump performance monitoring tool. This monitoring tool is of course very helpful in facilitating production operators in water treatment installations. Its purpose is to monitor pump performance and the coagulation process. When the pac liquid and raw water carry out the affixing process in the coagulant tanks in each installation building, which will later determine the results of the processed raw water. Therefore, the author created an IOT (Internet of things) Based Pac Dosing Pump Performance Monitoring Tool, which will be connected to other electronic modules such as adapter plugs, nodemcu esp8266, step down modules, relay sensors, mini water pumps, rain sensors, applications blynk, and the Samsung A3 2016 cellphone device. The next step is to program each component so that it is connected to each other, by nodemcu as the basis of the process which will produce input in the form of notifications to the user in the form of notifications on the device.
 
Keywords: Internet Of Thing, Nodemcu, Sensor Relay, Water Pump, Rain Sensor, Blynk.
Evaluasi Penggunaan Website Kampus Menggunakan Metode Usability Testing dan System Usability Scale
Authors: Ekawati Inna , Sumadyo Malikus , Whidhiasih Retno Nugroho
Published: November 2024

The campus website is the face of an institution that can be accessed online by everyone because the website is a central information service. When individuals such as potential students, the wider public, and those within the institution itself require information about the campus, they will primarily turn to the website as their main source. However, not all campus websites have a perfect role as information and service centers. The quality of a campus website can be seen from the perception and feedback of its users. Therefore, an assessment of online media usage, especially websites, is essential at all times to understand user perceptions of the ongoing website development. This research is intended to evaluate the usability of the website of Islamic University 45 Bekasi to obtain the best recommendations for improvement. In addition to the evaluation, this research also compares two methods of website usability evaluation. The methods to be used are usability testing and the System Usability Scale. Both methods are conducted simultaneously to obtain qualitative and quantitative data. This research yields recommendations for areas that need improvement or enhancement and also provides a comparison of the two methods used as references for usability evaluation. The research results reveal that with the first method, there is a significantly higher number of positive perceptions than negative ones, although there are many suggestions for improvement. On the other hand, when using the second method, the campus website exhibits the second least favorable usability score among a total of eleven levels.
STRATEGI GURU PENDIDIKAN AGAMA ISLAM DALAM MENGATASI KEJENUHAN BELAJAR PADA SISWA DI SMA NEGERI 10 GOWA
Authors: Novianti Irka , Malli Rusli , Muthahharah Sitti
Published: November 2024

In the education process, there are often cases, one of which is learning saturation.  The meaning of saturation is literally dense or very full, not only that, saturation can also mean boredom. If a learner in the educational process faces such a boredom incident, the matter may make the learner feel that he is wasting his time. Learning boredom is time spent studying, but there are no results. The purpose of this study was to determine and analyze the strategy of Islamic religious education teachers in overcoming learning saturation in students at SMA Negeri 10 Gowa. The research method applies a qualitative approach with the type of field research. This research uses primary sources derived from documentation and interviews with teachers and students of SMA Negeri 10 Gowa. The results showed that about the strategy of Islamic religious education teachers in overcoming learning boredom in students at SMA Negeri 10 Gowa through varied learning methods, doing ice breaking, building a conducive learning atmosphere, providing rewards, conducting learning outside the classroom and building family relationships between teachers and students
STRATEGI GURU PAI DALAM MENGATASI KESULITAN MEMBACA AL-QUR’AN PADA PESERTA DIDIK DI MTS ARIFAH GOWA
Authors: Saputra Syachrul Muh Ilham , Fattah Abdul , Rusyd Rajiah
Published: November 2024

The purpose of this study was to determine the learning of the Qur'an in students, the factors of students' difficulties in reading the Qur'an and the PAI teacher's strategy in overcoming difficulties in reading the Qur'an at MTs Arifah Gowa. This research method uses field research with a qualitative approach. The data needed in this study used the main instrument in the form of interviews, while observation and documentation were used to complement the data needed. All data collected were analysed in the form of data reduction, data presentation, and verification or conclusion drawing in a qualitative descriptive manner. The results showed that PAI teachers at MTs Arifah Gowa used the lecture method, assignment method, iqro method, practice method of reading al-qur'an habituation. The method of learning Islamic religious education, especially in the subject of al-qur'an which is often used by teachers is the method of demonstration and lecture. The factors that influence the difficulty of reading the Qur'an are students' self-awareness and motivation, lack of attention and education from parents, the negative influence of high technology so that students are carried away with things that are not useful compared to learning to read the Qur'an. Students' difficulties in reading the Qur'an are not recognising the hijaiyah letters, it is difficult to distinguish the short length of the Qur'anic recitation. The strategy is carried out to foster students, namely at the beginning of each lesson the teacher does tahsin qiraah or corrects the reading of the Qur'an.
El Himno de la Novia en Acta Thomae. Un nuevo acercamiento al texto griego
Authors: Lesage-Garriga Luisa
Published: November 2024

El Himno de la Novia (capítulos 6-7 de los Hechos de Tomás), cuenta con una historia del texto particular que lo distancia de la tradición de otras partes del texto. El presente estudio se hace eco de su compleja tradición textual y ofrece un análisis de 19 manuscritos que lo transmiten.
Use of Social Media in Adolescents as a Trigger for Bullying Behavior: A Study on High School Students in Bogor
Authors: Ratih Siti Aminah  , Permana Restiawan
Published: November 2024

Bullying that occurs at the high school level is very urgent to get an immediate solution. This is considering that high school students are potential successors to development. Indonesia will enter the golden period, in 2045. Adolescents who are free from bullying will be ready to participate in development. Internet media is one of the triggers for bullying. Therefore, it is very important to know the use of adolescent internet media in relation to bullying.This study analyzes the relationship between adolescent social media use and bullying behavior among high school students in Bogor City. Data was collected from 100 respondents with an age range of 17-19 years, consisting of 57% women and 43% men. The analysis showed a significant relationship between adolescent characteristics and communication with peers (β = 3.225), as well as a relationship between adolescent characteristics and social media use (β = 1.835). In addition, a relationship was also found between adolescent characteristics and bullying behavior (β = 0.780). These results underscore the importance of paying attention to factors such as adolescent characteristics and interaction with social media in understanding bullying behavior among adolescents. These findings highlight the need for a holistic approach to the prevention and management of bullying behaviors that includes parental supervision as well as a better understanding of adolescents' social media use. Social media has a significant role in shaping adolescents' attitudes regarding bullying, family communication contributes to the occurrence of bullying behavior in adolescents.
Influence of Electronic Word of Mouth on Labuan Bajo Content and Celebrity Endorser on TikTok Account @tassy.sy on Generation Z's Visiting Interest
Authors: Putri Irawan Ayra Sekar , Intyaswati Drina
Published: November 2024

The recovery of the tourism sector in Indonesia, especially Labuan Bajo, can be overcome by utilizing technological developments and social media such as TikTok. Tassy is one of the traveling content creators who often shares traveling content on TikTok. This study aims to determine how much influence electronic word of mouth (variable X1 ) Labuan Bajo content and celebrity endorsers (variable X2 ) on the TikTok @tassy.sy account on visiting interest (variable Y) Generation Z. The theory used in this research is Source Credibility Theory. The research method used is explanatory quantitative. The sample technique uses Purposive Sampling by distributing questionnaires through TikTok. The research population is followers of the TikTok account @tassy.sy and obtained a sample size of 100 results from the Taro Yamane formula. The results of multiple linear regression analysis show that E-WOM and celebrity endorsers each have a positive and significant effect on visiting interest, with the magnitude of the influence of celebrity endorsers (0.827) greater than E-WOM (0.729). Meanwhile, the interaction between E-WOM and celebrity endorsers has a negative effect on interest in visiting, meaning that the knowledge gained through celebrity endorser accounts on TikTok elaborated with E-WOM related to Labuan Bajo does not increase interest in visiting. The coefficient of determination shows that the contribution of E-WOM on Labuan Bajo content and celebrity endorsers on the @tassy.sy TikTok account to visiting interest is 92.3%.
Caring for calves: Canadian public perspectives of calf handling methods during spring processing
Authors: Goldhawk Christy , Adams Cindy , Pajor Ed
Published: November 2024

In North American beef production, handling and restraint of young calves is integral to animal welfare and management practices. This study used a mixed-method approach to gather public perceptions of three handling and restraint methods common in western Canada during spring processing (TT—tilt table, RW—roping and wrestling, and NF—roping and NordFork). Canadians (n = 551) participated in an online survey that included videos of each handling method to ascertain preferences and acceptability. Participants were given industry information about handling and restraint or generic information regarding hay as a control information statement within the topic of agriculture. The survey also collected information about knowledge of the beef industry, animal welfare, and empathy toward animals. The reasons for preferences for specific handling methods were described as the presence of a perceived positive attribute and the absence of a perceived negative for most preferred methods, and inversely when explaining the least preferred method. The main themes focused on the calf’s experience, perception of handler actions, and pragmatic balancing of needs for a good life for the calf. All methods were rated as more acceptable for participants that ate meat consistently, knew more about the beef industry, and, to a lesser extent, if the individual had a lower animal empathy score. Acceptability was not affected by providing information about the practices; however, information did elicit more pragmatic reasoning. Most participants preferred TT over NF and RW (p < 0.001) and found TT more acceptable as well (p < 0.001). The TT was the most preferred method due to calf experience and human handling—notably the absence of dragging a calf, which was predominant in why participants selected NF or RW as their least preferred method. Consistency of themes highlights that regardless of method or acceptability, the fundamental expectation of the public focuses on the perception of the calf’s quality of life, humane handling, and pragmatism, which are values aligned with beef sustainability initiatives.
Investigating the efficacy of uncrosslinked porcine collagen coated vascular grafts for neointima formation and endothelialization
Authors: Yang Chao , Su Chao , Zou Jie , Zhong Binru , Wang Lin , Chen Bailang , Li Jianmo , Wei Minxin
Published: November 2024

Introduction

This study evaluates the efficacy of uncrosslinked porcine collagen coated vascular grafts (UPCCVG) in facilitating neointima formation and endothelialization.

Methods

Prior to coating, the uncrosslinked porcine collagen underwent comprehensive characterization employing SDS-PAGE, image analysis, circular dichroism and immunogenicity. The PET substrate of the vascular graft was coated with collagen solution utilizing the dip-coating method. Water permeability, blood leakage resistance, radial compliance, hemolysis, cytotoxicity and cell proliferation of UPCCVG in vitro were studied. Subsequent in vivo evaluation involved the implantation of UPCCVG as a substitute for the porcine abdominal aorta. Digital subtraction angiography (DSA) was employed to evaluate UPCCVG patency post-implantation, while histology, immunohistochemistry, and scanning electron microscopy were utilized to assess neointima formation and endothelialization. The in vivo thrombosis of UPCCVG was analyzed simultaneously to further characterize its blood compatibility.

Results

The uncrosslinked collagen demonstrated high purity, maintaining its triple helix structure and molecular weight akin to the type I bovine collagen standard substrate, indicative of preserved biological activity and low immunogenicity. UPCCVG exhibited water permeability, blood leakage resistance, radial compliance and blood compatibility comparable to commercial grafts. DSA revealed satisfactory patency of UPCCVG without evidence of stenosis or swelling at the 3-week post-implantation mark. Histological analysis illustrated well-developed neointima with appropriate thickness and controlled proliferation. Immunohistochemistry confirmed the presence of endothelial cells (VWF positive) and smooth muscle cells (α-SMA positive) within the neointima, indicating successful endothelialization. Moreover, the morphology of the neointima surface closely resembled that of the natural artery tunica intima, oriented along the direction of blood flow.

Discussion

UPCCVG, composed of uncrosslinked porcine collagen, demonstrates promising potential in fostering neointima formation and endothelialization while mitigating intimal hyperplasia. This biocompatible uncrosslinked porcine collagen merits further investigation for its clinical applications in vascular reconstruction.
POCOP-Ni(II) pincer compounds derived from phloroglucinol. Cytotoxic and antioxidant evaluation
Authors: Amaya-Flórez Andrés , Serrano-García Juan S. , Ruiz-Galindo Jordi , Arenaza-Corona Antonino , Cruz-Navarro J. Antonio , Orjuela Adrian L. , Alí-Torres Jorge , Flores-Alamo Marcos , Cano-Sanchez Patricia , Reyes-Márquez Viviana , Morales-Morales David
Published: November 2024

POCOP-Ni(II) pincer compounds have primarily been explored as catalysts, but their potential biological activity has been scarcely studied. To address this gap, we evaluated the anticancer and antioxidant potential of four POCOP-Ni(II) complexes derived from phloroglucinol. A comprehensive supramolecular analysis, based on single-crystal X-ray diffraction (DRX) structures, was conducted using Hirshfeld surfaces and non-covalent interaction analysis. The cytotoxicity of all complexes was systematically assessed against various cancerous cell lines, as well as a non-cancerous cell line (COS-7). The results revealed that complexes 1b and 1c exhibited remarkable antiproliferative activity, with IC50 values ranging from 2.43 to 7.85 μM against cancerous cell lines U251, K562, HCT-15, MCF-7, and SK-LU-1. To further elucidate their mechanism of action, a competitive fluorescence displacement assay with ethidium bromide (EB) suggested that these complexes possess the ability to intercalate with DNA. This multifaceted investigation not only enhances our understanding of the biological potential of POCOP-Ni complexes but also provides valuable insights into their structural features and interactions, paving the way for future exploration in both catalytic and therapeutic domains.
Victim impact statements limit the effects of perspective taking on juror decisions
Authors: Skorinko Jeanine Lee McHugh , Laurent Sean , Bendremer Emily , Schneider Kaitlyn , Hale Valicia , Iannacchione Alisionna , Paris Melissa , Demircioglu Gizem , Wesley Kyla , LaValley Julia , DeFreitas Angelica , Blumenthal Jeremy
Published: November 2024

Past work suggests that victim impact statements (VISs) encourage jurors to take victims' perspectives, but this has not been explored empirically. Across four experiments (total N = 881), the present research examines the effects of perspective taking and the impact of the crime expressed in VISs on juror perceptions of defendants. In Experiment 1, mock jurors read a capital murder case that prompted them to perspective take (or not) and included VISs that were high or low in impact of the crime on the victims. Results indicate that the impact of the crime expressed in the VISs influenced perceptions of culpability for the defendant, but perspective taking did not. Experiment 2 used an armed robbery case, and the results again showed that the higher impact of the VIS led to seeing the defendant as more culpable, but perspective taking did not. Experiment 3 examined whether the type of perspective taking mattered (imagining self vs. defendant) when VISs were also presented. Those using the self during perspective taking found the defendant less culpable compared to non-perspective takers. Experiment 4 examined whether priming perspective taking influenced decisions. While high-impact VISs resulted in more death penalty sentences than low-impact VISs, priming perspective taking did not. Overall, the impact of the crime expressed in VIS typically influenced the perceptions of the defendants. In contrast, perspective taking had limited effects. These findings contribute to our understanding of VISs in the courtroom and may be useful for attorneys when presenting cases and advising clients.
Efficacy and safety of sacubitril/valsartan on postoperative atrial fibrillation in adult patients undergoing cardiac surgery: a real-world observational study
Authors: Chen Xiaodong , Liu Pengxin , Zhu Fengzheng , Wang Dong , Yang Sumin , Yan Wenlong
Published: November 2024

Background

The mechanism underlying new-onset postoperative atrial fibrillation (POAF) in adult cardiac surgery is not well understood. However, efficient pharmacological methods to prevent and treat arrhythmic complications are still lacking. In the present study, we explored the efficacy and safety of sacubitril/valsartan (sac/val) in the control of POAF in adult cardiac surgery patients.

Methods

Between January 2021 and December 2021, 667 eligible adult patients who underwent cardiac surgery at the Affiliated Hospital of Qingdao University were enrolled. The participants were divided into two groups according to whether sac/val was used: the sac/val group (N = 101) and the control group (N = 566). The main observational endpoints were the incidence of POAF, left ventricular ejection fraction (LVEF) recovery, in-hospital mortality, and short-term mortality.

Results

Patients in the sac/val group had a lower incidence of POAF than those in the control group (26/101 vs. 204/566, P = 0.045). Patients in the sac/val group also showed a higher communicative risk for POAF incidence using the Kaplan–Meier survival analysis. In addition, patients in the sac/val group showed better LVEF recovery, with dynamic changes in LVEF superior to that of the control group. The change in LVEF in the sac/val group was 1.78 ± 5.41, compared with −1.19 ± 10.92 in the control group (P = 0.008).

Conclusions

This is the first observational study to evaluate the efficacy and safety of sac/val in the prevention and treatment of POAF after cardiac surgery. The results demonstrated that compared with patients who did not receive sac/val treatment, those who received Sac/val treatment showed better POAF control and LVEF recovery. These results should be cautiously interpreted and further confirmed using larger sample sizes and prospective randomized controlled trials.
Case Report: Diverticulum of the left ventricular outflow tract: multimodal imaging
Authors: Chen Qiqing , Liu Lili , Cao Yiqiu , Chen Pian , Zhong Wenjin , Jing Xiangxiang
Published: November 2024

The left ventricular diverticulum (LVD) is a rare congenital heart disease commonly discovered during examinations. Herein, we report a LVD on the lateral wall of left ventricular outflow tract. A case of a 22-year-old female patient who visited our hospital with complaints of shortness of breath, accompanied by vomiting, cough, and lower limb and facial edema and was diagnosed with “aortopulmonary septal defect” using transthoracic echocardiography. After cardiac surgery and postoperative pathology, she was confirmed with a LVD. The patient's clinical symptoms, multimodal imaging examination, and treatment methods were used to assess this case.
Practical application of precision oncology in adult onset craniopharyngiomas
Authors: Biswas Chandrima , Mansur Guilherme , Wu Kyle C. , Prevedello Daniel M. , Ghalib Luma
Published: November 2024

Introduction

Craniopharyngiomas (CPs) are benign and rare tumors found in adults. Their location close to vital neurovascular structures makes traditional treatment modalities (surgery and radiation) challenging and potentially fraught with morbidity. The 2021 WHO classification has divided what was previously considered two subtypes of craniopharyngioma into separate entities. Identification of specific molecular driver mutations in each type- BRAF V600E in papillary craniopharyngiomas (PCP) and CTNNB1 in adamantinomatous craniopharyngiomas (ACP) has resulted in a paradigm shift in the management of adult CPs.

Methods

In this study, we describe our experience in treating PCPs with targeted therapy and highlight nuances in management accounting for current evidence. This review also explores the current scope and application of precision oncology in adult CPs including the experience with ongoing trials and prospects for future research.

Results

The high prevalence of targetable mutation in cases of PCP and the efficacy of BRAF inhibitors alone or in combination with MEK inhibitors has improved the disease control in these patients. In the current scenario, while surgery is warranted to obtain histopathological diagnosis, radical resection and its associated risks can be avoided. In case of ACPs, dysregulation of multiple pathways has been implicated. This has prompted the use of a variety of targeted therapies with inconsistent outcomes. The results of ongoing and future trials may define its role in management.

Conclusion

Precision oncology is a promising addition to the treatment armamentarium of adult CPs.
Optimal dispatch of an electricity-heat virtual power plant based on Benders decomposition
Authors: Wu Yunxia , Yang Shenhao , Zhang Su , Li Longyuan , Yan Xiaobin , Li Jingxuan
Published: November 2024

Recently, wind energy has been developed as an important technology to address the energy crisis. However, due to an unreasonable energy structure, wind power curtailment is becoming increasingly severe. Combined heat and power dispatch (CHPD) provided a solution for wind accommodation by utilizing the flexibility resources of district heating systems. Because of the imperfect dispatch methods and CHPD platforms, many wind power heating projects have not effectively linked the use of abandoned wind and heating. The virtual power plant (VPP) achieves the reasonable combination of controllable power sources, distributed energy, controllable loads, and energy storage systems within a certain area. Thus, we propose a VPP model based on combined dispatch of wind power and heat energy, which integrates wind turbines, thermal turbines, CHP units, etc., into a whole to join in the grid operation. Besides, to preserve the privacy of energy agents, Benders decomposition algorithm is adopted to solve the proposed model in this paper. The validity and efficiency of the proposed VPP model and Benders decomposition algorithm are verified via numerical cases.
Structure-adaptive canonical correlation analysis for microbiome multi-omics data
Authors: Deng Linsui , Tang Yanlin , Zhang Xianyang , Chen Jun
Published: November 2024

Sparse canonical correlation analysis (sCCA) has been a useful approach for integrating different high-dimensional datasets by finding a subset of correlated features that explain the most correlation in the data. In the context of microbiome studies, investigators are always interested in knowing how the microbiome interacts with the host at different molecular levels such as genome, methylol, transcriptome, metabolome and proteome. sCCA provides a simple approach for exploiting the correlation structure among multiple omics data and finding a set of correlated omics features, which could contribute to understanding the host-microbiome interaction. However, existing sCCA methods do not address compositionality, and its application to microbiome data is thus not optimal. This paper proposes a new sCCA framework for integrating microbiome data with other high-dimensional omics data, accounting for the compositional nature of microbiome sequencing data. It also allows integrating prior structure information such as the grouping structure among bacterial taxa by imposing a “soft” constraint on the coefficients through varying penalization strength. As a result, the method provides significant improvement when the structure is informative while maintaining robustness against a misspecified structure. Through extensive simulation studies and real data analysis, we demonstrate the superiority of the proposed framework over the state-of-the-art approaches.
Perspectives in newborn screening for SCID in Japan. Case report: newborn screening identified X-linked severe combined immunodeficiency with a novel IL2RG variant
Authors: Beppu Shiro , Nishikawa Takuro , Tomomasa Dan , Hijikata Atsushi , Kasabata Hiroshi , Terazono Hideyuki , Ikawa Kazuro , Nakamura Tatsuro , Horikawa Shogo , Nagahama Jun , Nakamura Aki , Abematsu Takanari , Nakagawa Shunsuke , Oketani Kaoru , Kanegane Hirokazu , Okamoto Yasuhiro
Published: November 2024

Background

Newborn screening (NBS) for severe combined immunodeficiency (SCID) has improved the prognosis of SCID. In Japan, NBS testing (measurement of the T-cell receptor excision circles (TREC) and kappa-deleting recombination excision circles (KREC)) was launched in 2017 and has expanded nationwide in recent years. In this study, we report a Japanese patient with X-linked SCID with a novel IL2RG variant identified through NBS. The patient underwent cord blood transplantation (CBT).

Case

The patient had no siblings or family history of inborn errors of immunity. He was born at 38 weeks of gestation and weighed 3,072 g. His NBS results revealed TREC 0 copies/105 cells (normal value: >565 copies/105 cells), which was considered suggestive of SCID. The patient was referred to our hospital. Although his lymphocyte count was 1,402/μL, naïve T cells and CD56+ natural killer (NK) cells were decreased to 0% and 0.05% of the total lymphocytes, respectively. Flow cytometric measurement testing revealed a decrease in γc protein expression in the B lymphocytes and NK lymphocytes. We identified a hemizygous novel missense variant (c.256A>C, p.Thr86Pro) of IL2RG. Both in silico and structural analyses revealed that this variant is likely pathogenic. At 3 months of age, he underwent CBT from a human leukocyte antigen-full-matched unrelated donor. The conditioning regimen included fludarabine (180 mg/m2) and targeted busulfan (35 mg×h/L). The patient achieved high-level donor chimerism and immune reconstitution, including B-cell function, at 13 months.

Conclusion

Using NBS, the patient was diagnosed as having X-linked SCID with a novel missense variant of IL2RG. Early diagnosis using NBS tests enables safe hematopoietic stem cell transplantation without complications such as infection. We also found that even SCID with novel variants can be accurately diagnosed using the NBS program. In Japan, the test uptake rate is approximately 80% due to the high number of self-funded screening tests, and it is hoped that the uptake rate will increase in the future.
Discovery and preclinical evaluation of BPB-101: a novel triple functional bispecific antibody targeting GARP-TGF-β complex/SLC, free TGF-β and PD-L1
Authors: Xu Wenxin , Xu Jieying , Li Pingcui , Xu Deyu , Cheng Hongjie , Zheng Huan , Zhang Li , Liu Mengmeng , Ye Siyuan , Jiang Mengshi , Yu Wenqi , Wang Jiabing , Ding Lieming
Published: November 2024

Background

In the tumor microenvironment (TME), the transforming growth factor-β (TGF-β) and programmed cell death receptor 1 (PD-1)/programmed death ligand 1 (PD-L1) signaling axes are complementary, nonredundant immunosuppressive signaling pathways. Studies have revealed that active TGF-β is mainly released from the glycoprotein A repetitions predominant (GARP)-TGF-β complex on the surface of activated regulatory T cells (Tregs), B cells, natural killer (NK) cells, and tumor cells. The currently available antibodies or fusion proteins that target TGF-β are limited in their abilities to simultaneously block TGF-β release and neutralize active TGF-β in the TME, thus limiting their antitumor effects.

Methods

We designed and constructed a bispecific, trifunctional antibody, namely, BPB-101, that specifically targets the GARP-TGF-β complex and/or small latent complex (SLC), active TGF-β, and PD-L1. The binding ability of BPB-101 to the different antigens was determined by ELISA, FACS, and biolayer interferometry (BLI). The blocking ability of BPB-101 to the TGF-β and PD-1/PD-L1 signaling axes was determined by reporter gene assay (RGA). The antitumor effect and biosafety of BPB-101 were determined in a transgenic mouse tumor model and cynomolgus monkeys, respectively. Stability assessments, including stability in serum, after exposure to light, after repeated freeze-thaw cycles, and after high-temperature stress tests had been completed to evaluate the stability of BPB-101.

Results

BPB-101 bound efficiently to different antigenic proteins: the GARP-TGF-β complex and/or SLC, active TGF-β, and PD-L1. Data showed that BPB-101 not only effectively inhibited the release of TGF-β from human Tregs, but also blocked both the TGF-β and PD-1/PD-L1 signaling pathways. In an MC38-hPD-L1 tumor-bearing C57BL/6-hGARP mouse model, BPB-101 at a dose of 5 mg/kg significantly inhibited tumor growth, with a complete elimination rate of 50%. Stability assessments confirmed the robustness of BPB-101. Furthermore, BPB-101 showed a favorable safety profile in nonhuman primate (NHP) toxicity studies.

Conclusion

BPB-101 is a potentially promising therapeutic candidate that may address unmet clinical needs in cancer immunotherapy, thus, BPB-101 warrants further clinical investigation.
Analysis of immunogenicity and purification methods in conjugated polysaccharide vaccines: a new approach in fighting pathogenic bacteria
Authors: Sheikhi Arya , Shirmohammadpour Mina , Mahdei Nasirmahalleh Nima , Mirzaei Bahman
Published: November 2024

Carbohydrates are commonly found in conjunction with lipids or proteins, resulting in the formation of glycoconjugates such as glycoproteins, glycolipids, and proteoglycans. These glycoconjugates are essential in various biological activities, including inflammation, cell-cell recognition, bacterial infections, and immune response. Nonetheless, the isolation of naturally occurring glycoconjugates presents challenges due to their typically heterogeneous nature, resulting in variations between batches in structure and function, impeding a comprehensive understanding of their mechanisms of action. Consequently, there is a strong need for the efficient synthesis of artificial glycoconjugates with precisely described compositions and consistent biological properties. The chemical and enzymatic approaches discussed in this paper present numerous research opportunities to develop customised glycoconjugate vaccines.