bluuebunny/crossref_metadata_2025 huggingface dataset:
LANGERHANS CELL HISTIOCYTOSIS: PENEGAKAN DIAGNOSIS
Authors: Wahyuanggradewi Elliana , Yuliasis Widiasri Kharisma , Giantoro Jeffrey , Indrastuti Niken , Trisnowati Niken
Published: June 2022

Langerhans cell histiocytosisÂ (LCH) merupakan suatu penyakit neoplasia inflamatif sel dendritik mieloidÂ langka dengan manifestasi klinis yang bervariasi, ditandai dengan adanya sel mononuklear positif terhadapÂ CD1a/S100/CD207 yang menginfiltrasi sistem organ termasuk kulit. Kejadian LCH di RSUP Dr Sardjito dariÂ tahun 2014â€“2019 sebanyak 11 kasus. Makalah ini melaporkan satu kasus LCH pada anak berusia 1,5 tahun dengan keluhan utama bintik-bintik merah di seluruh tubuh dan kulit kepala berkerak sejak 6 bulan yang lalu. Pemeriksaan status dermatovenereologis menunjukkan pada kepala, wajah, dada, punggung, kedua tangan dan kaki, serta punggung kaki nampak papul purpurik multipel tersebar, kepala tertutup krusta kekuningan, kuku tangan dan kaki tampak hiperkeratosis. Pada abdomen didapatkan hepatosplenomegali. Pada pasien ini dilakukan pemeriksaan histopatologis dan imunohistokimia S-100 didapatkan positif pada sitoplasma sel tumor dan CD1a positif pada sitoplasma dan membran sel tumor. Diagnosis LCH ditegakkan berdasarkan kondisi klinis dan histopatologis. Pasien mendapatkan terapi pelembab dan regimen kemoterapi.Kata kunci :Â Langerhans cell histiocytosis, diagnosis, imunohistokimia
PERANAN EDUKASI, TERAPI OKUPASI DAN FISIOTERAPI PADA MORBUS HANSEN TIPE MULTIBASILER DENGAN KECACATAN DERAJAT DUA
Authors: Widasmara Dhelya , Rasyidi Faradiani , Nugroho Muhammad Barlian
Published: June 2022

Kerusakan saraf pada kusta dapat menyebabkan cacat dan berdampak negatif pada hidup. RehabilitasiÂ medis penting pada pasien kusta, meliputi edukasi pemeliharaan kulit, proteksi tubuh, fisioterapi, bidai,Â orthoses, dan terapi okupasi. Dilaporkan satu kasus pria usia 35 tahun kontrol ke Poliklinik Kulit dan Kelamin Rumah Sakit Umum Daerah dr. Saiful Anwar (RSSA) Malang dengan tangan kanan kaku dan luka di ibu jari dan jari manis tangan kanan. Pasien didiagnosis Morbus Hansen Tipe Multibasiler (MHMB) dan kecacatanÂ derajat II (claw hand dextra) sejak Februari 2018. Pemeriksaan fisis menunjukkan claw hand dextra danÂ pemendekan ibu jari tangan kiri. Pemeriksaan dermatologis menunjukkan ulkus, batas tegas, jumlah dua dengan ukuran masing-masing 1 x 0,5 cm pada palmar manus dekstra. Pasien diterapi melanjutkan MDT MB dan edukasi rawat luka. Pasien dikonsultasikan ke Poliklinik Rehabilitasi Medik RSSA dan diterapi okupasiÂ dan fisioterapi selama lima kali. Didapatkan peningkatan kekuatan fungsi dan ketangkasan tangan kanan, sertaÂ peningkatan kekuatan otot abduksi jari kelingking kanan. Tidak ditemukan efek samping pada pasien.Kata kunci :Â Kecacatan derajat II, morbus hansen tipe multibasiler, rehabilitasi medik
HUBUNGAN ANTARA PEKERJAAN SEBAGAI TENAGA KESEHATAN DENGAN KEJADIAN DERMATITIS KONTAK AKIBAT KERJA
Authors: Batubara Irwan Saputra , Hardjodipuro Andira , Widaty Sandra
Published: June 2022

Latar belakang:Â Sebanyak 95% penyakit kulit akibat kerja merupakan dermatitis kontak, yang mencakup dermatitis kontak alergi dan iritan. Beberapa jenis pekerjaan tertentu memiliki frekuensi lebih tinggi untuk terpajan dengan bahan atau aktivitas yang meningkatkan risiko kejadian dermatitis kontak akibat kerja, termasuk tenaga kesehatan.Â Tujuan:Â Menilai hubungan antara pekerjaan sebagai tenaga kesehatan dengan kejadian dermatitis kontak akibat kerja melalui pencarian informasi berbasis bukti.Â Metode:Â Pencarian artikel dilakukan menggunakan PubMed, Cochrane Library, Proquest dan Scopus dengan kata kunci yang sesuai dengan pertanyaan klinis. Artikel yang diperoleh diseleksi menurut kriteria inklusi dan eksklusi yang kemudian ditelaah kegunaannya berdasarkan nilai validity, importance, dan applicability.Â Hasil:Â Didapatkan dua artikel berupa studi kohort retrospektif dan studi kasus-kontrol. Pada studi kohort retrospektif didapatkan bahwa pekerjaan sebagai tenaga kesehatan memiliki risiko 1,17 kali lebih besar untuk mengalami dermatitis kontak akibat kerja dibandingkan dengan pekerjaan selain tenaga kesehatan (RR 1,17, nilai p <0,001, NNH 3). Hal ini didukung oleh hasil studi kasus-kontrol yang memberikan hasil OR sebesar 2,5, CI 95% 2,08 â€“ 3,02 dan NNH 5.Â Kesimpulan:Â Risiko terjadinya dermatitis kontak pada tenaga kesehatan lebih tinggi dibandingkan dengan kelompok pekerja lainnya sehingga diperlukan upaya untuk menurunkan angka kejadian tersebut.Kata kunci :Â Tenaga kesehatan, perawat, dermatitis kontak akibat kerja, dermatitis kontak alergi, dermatitis kontak iritan
DERMOSKOPI PADA PENYAKIT INFLAMASI KULIT
Authors: Andardewi Melody Febriana , Krisanti Roro Inge Ade , Budianti Windy Keumala , Effendi Evita Halim
Published: June 2022

Penegakkan diagnosis penyakit inflamasi kulit dilakukan berdasarkan anamnesis, gambaran morfologi,Â serta distribusi dari lesi. Namun, terkadang klinisi kesulitan untuk menyingkirkan diagnosis banding penyakit yang dialami oleh pasien. Dermoskopi merupakan alat diagnostik non-invasif yang dapat digunakan untuk membantu diagnosis kelainan kulit. Dermoskopi dapat membantu visualisasi struktur di bawah permukaanÂ kulit hingga ke dermis superfisialis dan memperlihatkan morfologi lesi yang sulit teramati secara kasatÂ mata. Pada awalnya dermoskopi dipakai sebagai pemeriksaan penunjang untuk tumor jinak dan tumorÂ ganas kulit. Saat ini, dermoskopi digunakan secara luas dalam berbagai penyakit kulit antara lain penyakit infeksi dan infestasi kulit (entomodermoscopy), kelainan kuku dan lipat kuku (onychoscopy), kelainan rambut (trichoscopy), penyakit inflamasi kulit (inflammoscopy), serta membantu pengambilan keputusan dan evaluasi terapi. Pemeriksaan dermoskopi pada penyakit inflamasi kulit meliputi pengamatan morfologi dan distribusi pembuluh darah, warna dan distribusi skuama, gambaran folikuler, struktur lain, serta tanda spesifik yang dapat ditemukan pada penyakit tertentu. Dengan menggabungkan hasil anamnesis, pemeriksaan fisik, serta pemeriksaan dermoskopi, diagnosis penyakit inflamasi kulit menjadi lebih akurat.Kata kunci :Â dermoskopi, inflammoscopy, penyakit inflamasi kulit
TERAPI CONDITIONED MEDIUM-WHARTONâ€™S JELLY-MESENCHYMAL STEM CELL PADA ULKUS TROFIK KUSTA TIPE MULTIBASILAR LEPROMATOSA
Authors: Nathasia Nathasia , Tan Sukmawati Tansil
Published: June 2022

Ulkus trofik adalah luka akibat trauma kecil, sering ditemukan di daerah plantar dan titik penekanan yang dialami pasien kusta. Conditioned medium-Whartonâ€™s jelly-mesenchymal stem cells (CM-WJ-MSCs) atau secretome, selanjutnya disebut Conditioned medium dihasilkan dari media pertumbuhan sel induk yang digunakan. Conditioned medium dapat mempengaruhi seluruh fase penyembuhan luka, mempunyai efek imunomodulasi, antiinflamasi, anti-apoptosis, neuroprotektif dan neurotropik, regulasi angiogenesis, antitumor, dan antimikroba, sehingga mempercepat penyembuhan luka dan perbaikan jaringan. Conditioned medium dianggap sebagai â€œcell free therapeuticsâ€. Dilaporkan 2 kasus pasien kusta tipe MB kasus pertama seorang laki-laki berusia 53 tahun, dengan luka telapak kaki kiri sejak 10 tahun, ulkus berukuran 4 x 2 x 0,6cm3 dan berbatas tegas; Kasus kedua, pria berusia 52 tahun, dengan luka jari telunjuk tangan kanan sejak tiga bulan, ulkus berukuran 1,5 x 1,5 x 0,5cm3 dan berbatas tegas. Kedua pasien pernah menderita kusta tipe multibacillar lepromatous Leprosy (MBLL) dan telah menyelesaikan pengobatan selama 12 bulan. Tata laksana ulkus pada kedua pasien adalah dengan pemberian conditioned medium-WJ-MSCs gel 10% dan dipantau selama tiga minggu. Pengamatan hari ke-10, ukuran ulkus mulai mengecil. Pada hari ke-20, ulkus semakin menutup. Pada minggu ke-tiga pengobatan dihentikan dan pasien diberikan sejumlah gel untuk dilanjutkan terapi mandiri. Berdasarkan laporan terapi hasil pada kedua kasus tersebut conditioned medium-WJ-MSCs merupakan terapi yang efektif untuk penyembuhan ulkus trofik.
PERBANDINGAN PENCAPAIAN PASI-75 NARROWBAND ULTRAVIOLET B DENGAN METOTREKSAT ORAL PADA PSORIASIS VULGARIS DERAJAT SEDANG-BERAT
Authors: Giantoro Jeffrey , Waskito Fajar , Radiono Sunardi
Published: June 2022

Latar belakang: Psoriasis merupakan penyakit inflamatorik kronis kambuhan yang mengganggu kualitas hidup. Fototerapi narrowband ultraviolet B (NBUVB) dan metotreksat (MTX) adalah pilihan terapi psoriasis vulgaris (PV) derajat sedang-berat. Tujuan: Membandingkan efektivitas NBUVB dengan MTX oral sebagai terapi PV. Metode: Penelitian analitik retrospektif terhadap 19 pasien yang didapat datanya dari rekam medis tentang efektivitas terapi berdasarkan skor Psoriasis Area and Severity Index (PASI) sejak Maret 2019 hingga Agustus 2019 di RSUP dr. Sardjito Yogyakarta. Pasien dibagi menjadi kelompok NBUVB (8) dan MTX (11). Penurunan PASI sebesar 75% (PASI-75) merupakan indikator keberhasilan. Hasil: PASI-75 dicapai 6 (75%) orang pada kelompok NBUVB, sementara kelompok MTX 9 (81,82%) orang. Rata-rata jumlah minggu pencapaian PASI-75 adalah 11,67 Â± 2,67 pada kelompok NBUVB serta 17,33 Â± 4,89 pada kelompok MTX (p=0.023). Diskusi: Pencapaian PASI-75 pada MTX lebih lama karena waktu paruh obat sangat panjang disertai frekuensi pemberian per minggu sehingga onset lebih lambat. Kepatuhan berobat dan pemantauan berkala menjadi kunci sukses terapi. Sampel yang besar dan waktu observasi yang panjang diperlukan untuk penelitian yang lebih baik. Kesimpulan: NBUVB memberikan penurunan PASI lebih cepat, sedangkan MTX memberikan hasil maksimal dalam jangka panjang. Keduanya memiliki anti mitotik, anti proliferasi, serta anti inflamasi.Kata kunci: psoriasis, metotreksat, ultraviolet, fototerapi, pasi
SEROLOGI SIFILIS PADA INFEKSI HUMAN IMMUNODEFICIENCY VIRUS (HIV)
Authors: Priyanto Mufqi Handaru , Marissa Melani , Indriatmi Wresti
Published: June 2022

ABSTRAKSifilis merupakan infeksi menular seksual (IMS) yang diketahui berhubungan dengan peningkatan risiko infeksiÂ human immunodeficiency virus(HIV) dan begitupun sebaliknya. Hingga saat ini, deteksi sifilis dengan atau tanpa koinfeksi HIV menggunakan tes serologi masih menjadi pilihan utama, terutama di negara-negara berkembang. Tes serologi yang rutin digunakan meliputi tes nontreponema dan treponema. Dalam menegakkan diagnosis, klinisi seringkali merujuk pada tes serologi tersebut. Hasil tersebut perlu menjadi perhatian karena manifestasi klinis dan tes serologi sifilis pada HIV sering tidak biasa. Hal ini diduga akibat respons imun pada infeksi HIV yang dapat mengubah perjalanan alamiah sifilis. Beberapa hasil tes serologi sifilis pada koinfeksi HIV dapat menunjukkan hasil negatif palsu, terjadinya serokonversi, dan adanya fenomenaÂ serofast. Selain itu, perlu juga dilakukan tes penapisan serologi sifilis secara rutin pada koinfeksi HIV dan untuk mengevaluasi efektivitas terapi. Berbagai pola serologi sifilis pada koinfeksi HIV yang tidak biasa tersebut perlu diantisipasi dengan baik sehingga tata laksana dapat berjalan efektif, dan diharapkan mengurangi risiko penularan terutama pada kelompok dengan risiko tinggi.
APLIKASI TERAPI OKSIGEN HIPERBARIK DALAM PENYEMBUHAN LUKA KRONIS
Authors: Sinambela Duma wenty irene , Ginting Oratna
Published: June 2022

ABSTRAKLuka kronis adalah luka yang membutuhkan waktu lama untuk sembuh, tidak sembuh, atau berulang. Luka kronis sebagian besar terkait dengan hipoksia dan iskemia yang dapat merusak sintesis kolagen dan dapat menyebabkan akumulasi metabolit seperti amonia yang menyebabkan pembengkakan sel dan mengganggu penyembuhan luka. Â Penyembuhan luka melibatkan banyak tipe sel yang berinteraksi, yang terdiri dari beberapa fase, yaitu fase hemostasis atau koagulasi, inflamasi, proliferasi, dan remodeling. Oksigen adalah nutrisi penting untuk luka dan memainkan peran penting dalam setiap tahap proses penyembuhan luka. Terapi oksigen hiperbarik ditujukan untuk mengatasi masalah mendasar dari hipoksia luka dengan menyediakan oksigen ke jaringan iskemik. Terapi oksigen hiperbarik merupakan penggunaan O2 100% pada tekanan lebih besar dari tekanan atmosfir. Pasien menghirup O2 100% secara intermiten sementara tekanan dari ruang perawatan ditingkatkan menjadi lebih dari 1 atmosfer absolut (ATA). Terapi oksigen hiperbarik mengurangi cedera reperfusi iskemia, memobilisasi Stem progenitor cell (SPC) yang telah diidentifikasi berperan dalam vaskulogenesis, meningkatkan aktivitas neutrofil untuk membunuh bakteri, menghasilkan reactive oxygen species (ROS) dan reactive nitrogen species (RNS), dan merangsang berbagai faktor pertumbuhan yang mendukung penyembuhan luka.Kata Kunci: luka kronis, HBOT, penyembuhan luka kronis, terapi oksigen hiperbarik
Sifilis Maligna Pada Pasien Human Immunodeficiency Virus
Authors: Setyowatie Lita , Puspitasari Galuh Dyah , Basuki Santosa
Published: June 2022

Sifilis maligna merupakan bentuk varian berat sifilis sekunder. Pada Pasien human immunodeficiency virus (HIV), perjalanan sifilis dapat menjadi atipikal dan lebih agresif, oleh karena itu varian ini sering ditemukan pada Pasien HIV. Mekanisme pasti perkembangan sifilis maligna hingga saat ini masih belum jelas, namun diduga berhubungan dengan imunosupresi, respon imun host yang tidak tepat, atau strain virulen Treponema pallidum. Ruam berupa papula dan plak berkrusta atau bersisik yang dapat berkembang menjadi ulkus atau lesi nekrotik (lesi rupioid), yang sering dikaitkan dengan tingginya titer nontreponemal dan disertai gejala sistemik. Berikut kami laporkan seorang Pasien HIV dengan plak nodul yang disertai ulseronekrotik tersebar di seluruh tubuh, serta memiliki hasil titer serologi sifilis yang tinggi. Berdasarkan gambaran klinis, serologi, dan patologi, Pasien didiagnosis sifilis maligna. Resolusi lesi kulit tampak signifikan setelah pemberian terapi Benzathine Penicillin. Dengan meningkatnya kasus koinfeksi sifilis dan HIV, penting untuk mengenali dan mendiagnosis sifilis maligna secara dini dan memberikan pengobatan yang tepat.Kata kunci : Sifilis maligna, Lues maligna, HIV, koinfeksi
GENERALIZED FIXED DRUG ERUPTION: SEBUAH LAPORAN KASUS JARANG
Authors: Saputra Joses , Rihatmadja Rahadi , Fitri Eyleny Meisyah , Novianto Endi , Dewi Cinthia Christina
Published: June 2022

Generalized fixed drug eruption (GFDE) merupakan varian klinis fixed drug eruption (FDE) yang muncul berupa lesi multifokal dengan pola sebaran lebih luas. Faktor pemicunya adalah pajanan berulang terhadap obat-obatan tertentu. Seorang laki-laki, 60 tahun, berobat untuk keluhan bercak merah gelap keunguan yang meluas pada sejumlah lokasi tubuh beberapa jam setelah minum obat bebas yang mengandung metampiron, tiamin, piridoksin, sianokobalamin, dan trimetilxantin. Konsumsi obat yang sama sebelumnya tidak menimbulkan keluhan, namun pasien mengalami ruam serupa terbatas pada beberapa lokasi tubuh enam tahun yang lalu setelah minum obat herbal, menyisakan bercak hiperpigmentasi. Pada pemeriksaan fisis didapatkan makula-plak merah gelap keunguan, makula hiperpigmentasi pekat, multipel, diskoid sampai oval, sirkumskrip-difus yang tersebar di seluruh tubuh. Pemeriksaan histopatologis menunjukkan interface dermatitis dan nekrosis epidermis, sesuai dengan FDE. Pemberian kortikosteroid oral setara prednison 0,5 mg/kgBB/hari menghilangkan ruam secara cepat. Temuan bercak merah gelap keunguan multifokal dengan pola sebaran luas saat ini dan hiperpigmentasi akibat reaksi di masa lalu, serta keterangan konsumsi obat tersangka sebelumnya, umumnya cukup untuk menegakkan diagnosis GFDE., Meskipun metampiron sangat dicurigai berdasarkan beberapa laporan kasus terdahulu substansi penyebab sebenarnya pada pasien belum dapat ditentukan hingga dibuktikan dengan hasil positif uji tempel obat.Kata kunci: generalized fixed drug eruption, hipersensitivitas obatÂ kortikosteroid, metampiron
ETIOPATOGENESIS KELAINAN KULIT KERING PADA DIABETES MELITUS TIPE 2
Authors: Legiawati Lili
Published: June 2022

Diabetes melitus tipe 2 (DMT2) merupakan penyakit metabolik yang disebabkan oleh peningkatan resistensi insulin, penurunan produksi insulin, atau kombinasi keduanya yang ditandai dengan hiperglikemia. Kelainan kulit kering termasuk salah satu manifestasi DMT2 dengan prevalensi tertinggi, yaitu 69%. Kondisi kulit kering, terutama pada kaki, berpotensi menjadi derajat yang lebih berat dan dapat berkembang menjadi ulkus kaki diabetes. Penanganan dini diperlukan untuk mencegah terjadinya penyulit dan peningkatan morbiditas pada penyandang DM. Kelainan kulit kering berkorelasi erat dengan pengontrolan glikemik yang buruk. Pada kondisi hiperglikemia dapat terjadi berbagai gangguan homeostasis kulit. Selain itu, faktor yang berkontribusi pada kelainan kulit kering adalah akumulasi advanced glycation end product (AGEs), kerusakan vaskular yang progresif, faktor neural, dan sistem imun. Etiopatogenesis kulit kering pada DMT2 meliputi berbagai hal, yaitu mekanisme terjadinya kulit kering secara sistemik, terdapatnya kondisi neuropati pada DMT2, dan perubahan biofisika pada stratum korneum. Di samping itu, terjadi penurunan hidrasi stratum korneum dan peningkatan TEWL, perubahan asam amino, lipid, dan pH kulit, serta penurunan proliferasi dan diferensiasi epidermis. Hal tersebut menyebabkan penumpukan lapisan korneosit dan penurunan konsentrasi lipid yang diduga menjadi faktor penyebab kulit kering pada penyandang DMT2. Â Â Kata kunci: AGEs, diabetes melitus tipe 2, hidrasi, hiperglikemia,Â  kulit kering
PERAN UJI TEMPEL OBAT NEVIRAPIN DALAM PELACAKAN PENYEBAB ERUPSI OBAT PADA PASIEN HIV
Authors: Handriani Risna , Indrastuti Niken , Pudjiati Satiti Retno
Published: June 2022

Erupsi obat (EO) saat ini masih menjadi masalah kesehatan karena kasusnya cukup tinggi. Penderita infeksi Human Immunodeficiency Virus (HIV) merupakan kelompok yang rentan mengalami EO. Salah satu pilihan terapi lini pertama HIV adalah nevirapin (NVP) yang diketahui paling sering menyebabkan EO. Manifestasi EO akibat nevirapin terbanyak, yaitu erupsi makulopapular, merupakan reaksi hipersensitivitas tipe IV. Cara menentukan obat penyebab EO seringkali hanya berdasarkan dugaan dari anamnesis. Uji tempel obat (UTO) merupakan uji kulit in vivo yang relative aman dan murah untuk mengidentifikasi penyebab EO yang didasari reaksi hipersensitivitas tipe IV.Tujuan penelitian ini untuk mengetahui persentase nilai positif UTO pada penderita HIV yang diduga mengalami EO karena nevirapin, serta menguji konsentrasi terbaik dan keamanan Â nevirapin yang dapat digunakan sebagai alergen UTO.Penelitian ini merupakan suatu penelitian deskriptif non-analitik. Uji tempel obat nevirapin dilakukan pada 25 subjek HIV dengan EO yang diduga karena nevirapin. Alergen nevirapin menggunakan pelarut vaselin album dengan konsentrasi 10%, 20%, dan 30%. Proporsi nilai positif UTO nevirapin tertinggi pada konsentrasi 30%, didapatkan pada 32% subjek, diikuti konsentrasi 20% pada 24% subjek dan konsentrasi terendah, yaitu 10% pada 20% subjek. Tidak ditemukan efek samping pada UTO nevirapin. Uji tempel obat nevirapin dengan konsentrasi 30% pelarut vaselin album berguna dan aman untuk mengidentifikasi alergi obat yang diduga karena nevirapin pada penderita HIV.Â Kata kunci: erupsi obat, Â uji tempel obat, nevirapin, HIV
KELAINAN IMUNODEFISIENSI PRIMER DI BIDANG DERMATOLOGI
Authors: Amalia Rhida Sarly , Budianty Windy Keumala , Kurniati Nia , Effendi Evita Halim
Published: June 2022

Kelainan imunodefisiensi primer merupakan kelompok penyakit heterogen dengan gangguan sistem imun yang disebabkan oleh defek genetik. Kelainan tersebut diklasifikasikan berdasarkan defek imunitas adaptif dan bawaan. Meskipun manifestasi klinisnya bervariasi, sebanyak 40-70% pasien menunjukkan gejala di kulit sehingga temuannya dapat membantu menegakkan diagnosis. Kelainan imunodefisiensi primer paling banyak terjadi pada bayi baru lahir hingga usia kurang dari satu tahun, namun dapat juga pada usia yang lebih tua. Kelainan imunodefisiensi primer dapat menyebabkan komplikasi infeksi rekuren dan berat, ataupun komplikasi non-infeksi. Dermatitis atopik merupakan temuan paling sering di kulit. Pasien dengan infeksi telinga, sinus, paru yang berulang dalam satu tahun, gagal tumbuh, respons buruk terhadap antibotik, sariawan persisten, abses kulit yang sulit sembuh, riwayat keluarga dan penyakit autoimun multipel harus dicurigai menderita kelainan imunodefisiensi primer. Pemeriksaan lymphocyte proliferation assay, flow cytometry, pengukuran kadar imunoglobulin serum, neutrophil function assay, dan uji komplemen dibutuhkan untuk mengonfirmasi diagnosis. Berbagai manifestasi kulit pada kelainan imunodefisiensi primer penting diketahui untuk membantu deteksi dini dan tata laksana lebih awal serta komprehensif pada pasien.Kata kunci: autoimun, dermatologi, genetik, kelainan imunodefisiensi primer
BEBERAPA JENIS FITOESTROGEN SEBAGAI TERAPI UNTUK PENUAAN KULIT PADA PEREMPUAN PASCAMENOPAUSE
Authors: Ramadhiani Mutiara , Yusharyahya Shannaz Nadia , Astriningrum Rinadewi , Hestiantoro Andon
Published: June 2022

Penuaan merupakan proses perubahan dinamis yang ditandai oleh kemunduran progresif berbagai sistem tubuh disertai penurunan kapasitas fisiologis. Salah satu faktor intrinsik penuaan kulit pada perempuan adalah penurunan kadar hormon estrogen di dalam tubuh saat memasuki masa pascamenopause. Estrogen merupakan hormon yang memiliki peran penting dalam regulasi fisiologis kulit. Berkurangnya kadar estrogen dapat mempercepat proses penuaan kulit yang ditandai dengan penurunan ketebalan, elastisitas, dan hidrasi kulit. Selama ini pemberian terapi hormon estrogen terbukti dapat mengurangi gejala penuaan kulit pada perempuan pascamenopause, namun terapi ini berpotensi meningkatkan risiko terjadinya efek samping berat contohnyaÂ kanker payudara, kanker endometrium, dan kanker ovarium. Fitoestrogen merupakan senyawa nonsteroid berasal dari tanaman yang memiliki aktivitas biologik serupa dengan estrogen. Setiap jenis fitoestrogen memiliki aktivitas biologik yang berbeda satu sama lain. Studi terkini menunjukkan bahwa fitoestrogen dapat menjadi terapi alternatif untuk mengatasi penuaan kulit pada perempuan pascamenopause. Fitoestrogen yang diberikan baik secara oral maupun topikal terbukti dapat meningkatkan elastisitas, ketebalan, dan kelembaban kulit serta mengurangi pigmentasi dan proses inflamasi pada kulit.Â
CAULIFLOWER-LIKE APPEARANCE CUTANEOUS PAPILLOMA
Authors: Sinambela Duma wenty irene , Siregar Remenda
Published: June 2022

ABSTRAKCutaneous papilloma (CP) adalah neoplasma jinak jaringan ikat dermis, terdiri dari jaringan fibrosa longgar, dan tampak sebagai papula bertangkai sewarna kulit hingga hiperpigmentasi yang biasanya bersifat asimptomatis apabila tidak disertai adanya peradangan dan iritasi. Terdapat 3 tipe CP yang umum ditemui, yaitu furrowed papule CP, Â filiform CP dan bag-like CP. Pada tulisan ini dilaporkan suatu kasus cauliflower-like appearance CP yang merupakan gambaran klinis tidak biasa dari CP.Dilaporkan suatu kasus pada perempuan berusia 45 tahun dengan keluhan timbul benjolan yang berjonjot-jonjot pada kelopak mata bawah sebelah kanan sejak 3 tahun lalu. Benjolan tersebut berjonjot-jonjot, lunak, tidak terasa nyeri, tidak gatal dan tidak berdarah.Â  Pada pemeriksaan dermatologis pada regio orbita dextra (lower eyelid) tampak nodul hiperpigmentasi, soliter, permukaannya verukosus, dan teraba lunak. Ukuran 1cm x 1cm. Â Pada pemeriksaan dermoskopi polarized dan non polarized didapatkan gambaran cerebriform (brain-like) pattern dan comedo like opening. Hasil pemeriksaan histopatologi menunjukkan sediaan jaringan dengan pelapis epitel skuamous berlapis yang mengalami hiperkeratosis, akantosis dan papilomatosis. Stroma terdiri dari jaringan ikat fibrous yang diinfiltrasi sel-sel radang limfosit yang minimal dan tampak struktur adneksa kulit dalam batas normal dengan kesimpulan suatu cutaneous papilloma. Pasien selanjutnya ditatalaksana dengan bedah eksisi.Kata Kunci: Cauliflower-like appearance, Cutaneous papilloma, Skin tag
Mediterranean Diet Adherence in Adolescents of Different Cultures and Geographical Proveniences: A Pilot Study
Authors: Intorre Federica , Foddai Maria Stella , Venneria Eugenia
Published: June 2022

The objective of the present research was to evaluate if and to what extent the encounter between different cultures in a context of globalization may have influenced the adherence to the Mediterranean diet (MD) in adolescents. The study comprised a structured questionnaire packet that inquired about anthropometric data, demographic information and both dietary and lifestyle habits. The KIDMED questionnaire was used to evaluate the adherence to the MD. A total of 103 respondents, aged 10–24 years (39.8% born in Italy or first-generation immigrants from Mediterranean countries) were included in the study. The results showed the highest percentage of adolescents having a medium adherence to the MD (44.7%), without differences according to geographical area of origin. Furthermore, the study proved that a higher adherence to the MD was significantly associated with daily breakfast (p = 0.008) and the consumption of fruit and vegetables (p = 0.002 and p = 0.000, respectively). Volunteers representing some of the different cultures and geographical proveniences currently present in Italy were perfectly integrated regarding diet and lifestyle, aspects considered in this study. Our investigation suggests the existence of incorrect dietary habits. It is certainly necessary to implement public health policies targeting adolescents to promote healthier lifestyle choices; the nutritional patterns of the MD should be among these choices.
Vibro-Acoustic Modelling of Aeronautical Panels Reinforced by Unconventional Stiffeners
Authors: Fasulo Giovanni , Vitiello Pasquale , Federico Luigi , Citarella Roberto
Published: June 2022

The purpose of this work is to characterise the vibro-acoustic behaviour of rectangular flat panels reinforced by “unconventional” stiffeners. Such panels are being increasingly employed in the aircraft industry in the case of composite fuselage, so that the assessment of the most efficient and accurate numerical techniques and modelling procedures to correctly predict their dynamic and acoustic behaviour is required. To this end, an analytical method, available from literature, has been initially employed to investigate on the main attributes of sound transmission loss properties of stiffened panels driven by an acoustic diffuse field excitation. Based on existing commercial codes, different numerical techniques have been implemented and deeply examined to assess their potentiality and restrictions. Among them, the Hybrid method has been eventually identified as the best compromise in terms of accuracy and computational effort. The drawbacks of deterministic and even Hybrid numerical approaches for medium–high frequency vibro-acoustic analysis when dealing with large structures, make use of the pure SEA approach compulsory. In particular, a refined tuning of a specific feature made available within the employed SEA analysis environment when dealing with reinforced shells is implemented as a potential solution to overcome the complexity in correctly modelling the examined unconventionally stiffened panels.
Effect of Lifting Gas Diffusion on the Station-Keeping Performance of a Near-Space Aerostat
Authors: Li Jun , Ling Linyu , Liao Jun , Chen Zheng , Luo Shibin
Published: June 2022

During the long-endurance flight of a near-space aerostat, the characteristics of lifting gas diffusion have a great influence on the flight altitude adjustment and station-keeping performance. Thus, in this study, a lifting gas diffusion model and a dynamic model that consider thermal effects, which had not been studied in similar models before, were developed. The dynamic model and thermal model were validated by historic flight data, and the calculated lifting gas diffusion results were compared with the experimental data of other researchers. The variations in the flight endurance, flight altitude, lifting gas diffusion rate, and diffusion coefficient of a near-space aerostat were analyzed. The effects of the ratio of porosity to tortuosity and envelope radiation properties on the mass of the lifting gas and flight altitude were considered in detail. To analyze the effect mechanism of the ratio of porosity to tortuosity and the envelope radiation properties, the envelope and gas temperature, as well as the gas pressure, were studied. The results show that the lifting gas diffusion rate and diffusion coefficient are very sensitive to the change in the ratio of porosity to tortuosity and envelope temperature. The results obtained from the analysis of the lifting gas diffusion can lay a solid foundation for improving the flight performance of near-space aerostats and for providing improved design considerations for aerostats.
Friction and Wear Properties of Wheat Straw Powder Third Body on Steel-Steel Friction Pair
Authors: Lian Chenglong , Zhao Wei , Wang Shumei , Zhang Peng , Dong Xiaoying , Li Yongfeng
Published: June 2022

Third body plant fibers have an important influence on the friction and wear properties of metal friction pairs. In this study, wheat straw powder with different particle sizes was prepared, and a pin-on-disk friction tester was employed to evaluate the friction and wear properties of the wheat straw powder third body on steel friction pairs under various conditions. The results show that the straw powder played an isolation role in the steel–steel friction pair, which reduced the contact area between the pin and the disc and thus reduced the friction coefficient. Compared with the large particle size powder, the small particle size powder became embedded in the friction interface or in the groove caused by wear, buffered the friction stress, and reduced the friction coefficient and wear. When lubricants such as lubricating oil or lubricating grease were added, the friction coefficient was significantly reduced. Under the influence of lubricant, the particle size of straw powder had no significant effect on the wear. The results of this study can provide a reference for the third body friction and wear properties of straw powder and the design of friction pairs for metal parts.
Comparing a New Non-Invasive Vineyard Yield Estimation Approach Based on Image Analysis with Manual Sample-Based Methods
Authors: Victorino Gonçalo , Braga Ricardo P. , Santos-Victor José , Lopes Carlos M.
Published: June 2022

Manual vineyard yield estimation approaches are easy to use and can provide relevant information at early stages of plant development. However, such methods are subject to spatial and temporal variability as they are sample-based and dependent on historical data. The present work aims at comparing the accuracy of a new non-invasive and multicultivar, image-based yield estimation approach with a manual method. Non-disturbed grapevine images were collected from six cultivars, at three vineyard plots in Portugal, at the very beginning of veraison, in a total of 213 images. A stepwise regression model was used to select the most appropriate set of variables to predict the yield. A combination of derived variables was obtained that included visible bunch area, estimated total bunch area, perimeter, visible berry number and bunch compactness. The model achieved an R2 = 0.86 on the validation set. The image-based yield estimates outperformed manual ones on five out of six cultivar data sets, with most estimates achieving absolute errors below 10%. Higher errors were observed on vines with denser canopies. The studied approach has the potential to be fully automated and used across whole vineyards while being able to surpass most bunch occlusions by leaves.
Evaluating Sensor-Based Mechanical Weeding Combined with Pre- and Post-Emergence Herbicides for Integrated Weed Management in Cereals
Authors: Saile Marcus , Spaeth Michael , Gerhards Roland
Published: June 2022

Due to the increasing number of herbicide-resistant weed populations and the resulting yield losses, weed control must be given high priority to ensure food security. Integrated weed management (IWM) strategies, including reduced herbicide application, sensor-guided mechanical weed control and combinations thereof are indispensable to achieve this goal. Therefore, this study examined combinations of pre- and post-emergence herbicide applications with sensor-based harrowing and hoeing in cereals by conducting five field experiments at two locations in Southwestern Germany from 2019 to 2021. Each experiment contained an untreated control and a single post-emergence herbicide treatment as a comparison to these IWM treatments. The effects of the different IWM approaches on weed control efficacy (WCE), crop density, and grain yield were recorded. All experiments were set up in a randomized complete block design with four repetitions. Pre-emergence herbicide application combined with one-time harrowing and subsequent hoeing (Pre-Herb + Harr + Hoe) achieved the highest WCE (100%), followed by an approach of WCE (95%) for two-times hoeing. In contrast, a single pre-emergence herbicide application achieved the worst result with an average WCE of 25%. Grain yield was equal between all treatments in between 6 t ha−1 and 10 t ha−1, except for a single pre-emergence herbicide application, which achieved a 2.5 t ha−1 higher grain yield in winter wheat in 2021 that averaged 11 t ha−1, compared to the combination of Pre-Herb + Harr + Hoe that averaged 8.5 t ha−1. The results showed that it is possible to reduce and replace herbicides while achieving equivalent yield and WCE.
Overexpression of a Thioredoxin-Protein-Encoding Gene, MsTRX, from Medicago sativa Enhances Salt Tolerance to Transgenic Tobacco
Authors: Duan Xinhang , Wang Zhaoyu , Zhang Yu , Li Han , Yang Mei , Yin Hang , Cui Jing , Chai Hua , Gao Yonghang , Hu Guofu , Zhang Pan
Published: June 2022

Thioredoxin (TRX) is a small molecule protein that participates in the redox process and plays a decisive role in various functions of plants. However, the role of TRX in Medicago sativa (alfalfa), a widely cultivated perennial herb of legume, is still poorly understood. Here, we isolated MsTRX from alfalfa and determined the characteristics in improving salt tolerance by assaying the phenotype and physiological changes and the expression of stress-response genes in transgenic tobacco. The expression of MsTRX was similar in alfalfa roots, leaves, and inflorescences, and was downregulated in response to cold, drought, and salt treatment. The overexpression of MsTRX in tobacco promoted the accumulation of soluble sugar (SS) and proline; enhanced the activity of peroxidase (POD); and induced the upregulation of beta-amylase 1 (BAM1), lipid-transfer protein 1 (LTP1), candidate signal molecules/sensor relay proteins (CBSX3), superoxide dismutase [Cu-Zn] (Cu/Zn-SOD), superoxide dismutase [Mn] (Mn-SOD), protein gamma response 1 (GR1), dehydrin DHN1-like (ERD10B), and serine/threonine-protein kinase (SnRK2), as well as the downregulation of phyB activation-tagged suppressor1 (BAS1) and serine/threonine-protein kinase that phosphorylates LHCII protein 7 (STN7) under salt stress. These results indicated that MsTRX improves salt tolerance via maintaining osmotic homeostasis, scavenging reactive oxygen species (ROS), and regulating the transcription of stress-response genes in plants. In our study, we provided a new understanding of how MsTRX improves salt stress in plants and how MsTRX can be included in future breeding programs to improve salt tolerance in alfalfa.
Yield, Flower Quality, and Photo-Physiological Responses of Cut Rose Flowers Grafted onto Three Different Rootstocks in Summer Season
Authors: Kwon O-Hyeon , Choi Hyo-Gil
Published: June 2022

The thermal stress caused by high temperatures on cut rose flowers grown in greenhouses is a major environmental impact that reduces the yield of growing cut rose flowers during summer. To confirm the resistance of grafted cut rose flowers to high-temperature stress, roses were grown in a greenhouse during the summer season and analyzed for yield, quality, root activity, and photo-physiological characteristics. A morphological change was observed in the stomata of the grafted cut rose flowers, which were larger in size than the scion or rootstocks. As a result of cultivating cut rose flowers by lowering the temperature of the greenhouse through shading in summer, it was confirmed that all of the scions, rootstocks, and grafted cut rose flowers were not in a stressed state by observing the maximal quantum yield of primary photochemistry (FV/FM) values on the chlorophyll-a fluorescence. However, the rate of electron transport flux from the primary acceptor (QA) to the secondary acceptor (QB) per the photosystem II reaction center (ET0/RC) value was found to be significantly higher on grafted cut rose flowers, compared with that of the scions. The efficiencies of the photosynthesis rate, the transpiration rate, and the stomatal conductance were increased when grafted compared with non-grafted. When the root activity was confirmed by the formazan content, it was found that the root activity was improved grafting. Furthermore, when grafted, morphological changes such as flower size and the number of petals on spray roses were also observed. Although there was a difference depending on the type of rootstock, the yield of the grafted cut rose flowers increased by 11–20%, compared with the scion rose. Therefore, grafting cultivation during the summer season with high temperatures is an effective method in terms of photo-physiological response and yield.
Economic Assessment of Irrigation with Desalinated Seawater in Greenhouse Tomato Production in SE Spain
Authors: Martínez-Granados David , Marín-Membrive Patricia , Calatrava Javier
Published: June 2022

This study assesses the impact of irrigating with desalinated seawater (DSW) on the profitability of greenhouse tomato in south-eastern Spain, comparing different water-quality sources in both traditional sanding cultivation and soilless hydroponic production. The assessment is based on the combination of partial crop budgeting techniques with field data from the LIFE DESEACROP Project experimental activities. Our results show that the exclusive use of DSW for tomato production increases fertilization costs by 20% in soilless systems and by 34% in traditional sanding cultivation, and water costs by 30% in soilless systems and by 48% in traditional soil cultivation. As a result, production costs increase by 5% in soilless cultivation and 3% in soil cultivation, increases that are reduced when DSW is blended with brackish water. However, the lower salinity of DSW, compared with conventional water resources in the area, increases both crop yield and profitability. Soilless cultivation would also increase tomato profitability but only if good quality water is available. The materialization of the potential benefits of soilless production requires improving water quality through the increased use of DSW. Otherwise, the traditional sanding production system, better adapted to the area’s poor soils and bad quality water, would be more profitable.
Effect of Foliar Treatment with Aqueous Dispersions of Silver Nanoparticles on Legume-Rhizobium Symbiosis and Yield of Soybean (Glycine max L. Merr.)
Authors: Krutyakov Yurii A. , Mukhina Maria T. , Shapoval Olga A. , Zargar Meisam
Published: June 2022

Interest in the use of silver as a component of plant protection products and growth regulators appeared relatively recently with the development of methods for the effective stabilization of colloidal systems containing nanoparticles of this metal. In the present work, we studied the effect of foliar treatments with aqueous dispersions of silver nanoparticles stabilized by polyhexamethylene biguanide hydrochloride with an average diameter of 6 ± 1 nm and a zeta-potential of +47.4 ± 1.3 mV on legume-Rhizobium symbiosis, which largely determines the efficiency of soil nitrogen assimilation and the yield of soybean (Glycine max L. Merr.). Based on the results of a two-year field experiment, it was shown that treatments with low doses of silver nanoparticles lead to a significant increase in the number of root nodules and an increase in soybean yield. The observed biological effectiveness of silver nanoparticles dispersions is explained by an increase in the enzymatic activity of peroxidases and polyphenol oxidases in the terrestrial part of plants. It is very likely that the treatment with silver nanoparticles and the increase in peroxidase activity in non-infected parts of the plant lead to a more effective prevention of the penetration of rhizobacteria into the aboveground soybean organs, which, in turn, may be the reason for the observed decrease in the activity of peroxidase and polyphenol oxidase in parts of plant roots susceptible to rhizobia. The latter, as is known, contributes to an easier flow of the nodulation process and the development of legume–Rhizobium symbiosis.
The Mitochondrial Genome of Cylicocyclus elongatus (Strongylida: Strongylidae) and Its Comparative Analysis with Other Cylicocyclus Species
Authors: Gao Yuan , Zhang Zhonghuai , Wang Chunren , Zhao Kai
Published: June 2022

Cylicocyclus elongatus (C. elongatus) is one of the species in Cylicocyclus, subfamily Cyathostominae, but its taxonomic status in Cylicocyclus is controversial. Mitochondrial (mt) genome is an excellent gene marker which could be used to address the taxonomy controversy. In the present study, the complete mt genome of C. elongatus was determined, and sequence and phylogenetic analyses were performed based on mtDNA data to determine the classification of C. elongatus. The circular complete mt genome of C.elongatus was 13875 bp in size, containing 12 protein-coding genes (12 PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 non-coding regions (NCRs). The A + T content of C. elongatus complete mt genome was 76.64%. There were 19 intergenic spacers with lengths of 2–53 bp and 2 overlaps with lengths of 1–2 bp in the impact complete mt genome. ATT and TAA were the most common start and termination codons of 12 PCGs, respectively. Comparative analyses of mt genomes nucleotide sequence and amino acid sequence showed that there were higher identities between C. elongatus and five other Cylicocyclus, rather than with P. imparidentatum. Phylogenetic analyses based on concatenated nucleotide sequences of 12 PCGs of 23 species in the family Strongylidae showed that C. elongatus was closely related to Cylicocyclus species, rather than P. imparidentatum. We concluded that C. elongatus was a member in Cylicocyclus based on comparative and phylogenetic analyses of mt genome sequences. The data of the complete mt genome sequence of C. elongatus provide a new and useful genetic marker for further research on Cyathostominae nematodes.
Pet dogs’ Behavioural Reaction to Their Caregiver’s Interactions with a Third Party: Join in or Interrupt?
Authors: Karl Sabrina , Anderle Kristina , Völter Christoph J. , Virányi Zsófia
Published: June 2022

Pet dogs are promising candidates to study attachment-related and potentially jealousy-like behaviours in non-human animals, as they form a strong and stable bond with their human caregivers who often engage in affiliative interactions with diverse social partners. Nevertheless, it is still debated whether non-human animals are capable of experiencing such complex emotions. Even though caregivers frequently report observations of jealousy-like behaviours in dogs, behavioural studies in dogs have thus far led to contradictory results. Adding to this complexity, dogs appear extraordinarily skilled in understanding humans’ communicative behaviour and can flexibly and diversely interact with them in social contexts. Here, we aimed at investigating (1) whether dogs indeed respond in a jealousy-consistent manner when seeing their caregiver interact in an affiliative way with a remotely controlled, realistic-looking fake dog, or (2) whether they would rather synchronize their reaction to the fake dog with the caregiver’s behaviour, or (3) whether they respond directly to the caregiver without paying much attention to the third party. To address what drives the dogs’ behaviours in this triadic situation, we compared four groups of dogs who first observed and then joined the interaction of either the caregiver or a stranger greeting or medically examining the fake dog. We found that the dogs initially responded negatively or neutrally when the fake dog entered the room but changed to more positive reactions when the caregiver approached the fake dog, especially if initiating a positive interaction. When being released, more dogs showed friendly behaviours towards the fake dog when the caregiver—rather than the stranger—was interacting with it. At the same time, however, the dogs tried to block the interaction of the caregiver with the fake dog more often than the one of the stranger. In conclusion, we did not find clear evidence for jealousy-like behaviours in dogs during the human–fake dog interactions, but we observed indicators of behavioural synchronization with the caregivers, suggesting that the caregivers’ affiliative behaviours directed at a third party may more often facilitate positive than negative interactions in dogs.
Urinary Protein/Creatinine Ratio in Feline Medicine: Reasons to Perform It and Its Role in Clinical Practice—A Retrospective Study
Authors: Fidalgo Maria Ana , Leal Rodolfo Oliveira , Duarte-Correia José Henrique
Published: June 2022

This study aimed at understanding the reasons veterinarians conduct a urinary protein/creatinine ratio (UPCR) in cats, correlating it with signalment, dipstick proteinuria tests, and urine specific gravity (USG) and assessing its role in chronic kidney disease (CKD) diagnosis and monitoring. A retrospective study was conducted, including medical data from cats consulted between 2016 and 2018 in a veterinary teaching hospital and submitted to at least one UPCR measurement. A total of 140 cats were included: 35% non-proteinuric (UPCR < 0.2), 25% borderline proteinuric (0.2 < UPCR < 0.4), and 40% overtly proteinuric (UPCR > 0.4). In contrast to other studies, there was no association between UPCR and male reproductive status. UPCR was mainly requested for CKD diagnosis and monitoring. Correlation between UPCR and combined results from dipstick tests and USG was low and inconsistent. Proteinuric CKD cats had a worse outcome at both 6 (odds ratio (OR 4.04) and 12 months (OR 4.36)), and this finding was more pronounced for severely proteinuric cases in which the OR for death was 4.36 and 6.00 at 6 and at 12 months, respectively. In addition to reinforcing the negative prognostic value of proteinuria, this study stresses the low and the inconsistent agreement between UPCR and the combined results of dipstick tests and USG in cats.
Biogenic Amines in Poultry Meat and Poultry Products: Formation, Appearance, and Methods of Reduction
Authors: Wójcik Wojciech , Łukasiewicz-Mierzejewska Monika , Damaziak Krzysztof , Bień Damian
Published: June 2022

Poultry meat is a source of many important nutrients, micro- and macro-elements, and biologically active substances. During meat storage, many physicochemical changes take place, also affecting the content of biologically active substances, including biogenic amines.They are formed as a result of three processes: decarboxylation of amino acids by microorganisms, reductive amination, and transamination of aldehydes and ketones, and as a result of activity of body tissues. Excessive consumption of biogenic amines shows toxic properties. The increasing consumption of poultry meat and the lack of established limits for biogenic amine content is a major challenge for scientists, producers, and consumer organisations, which have not yet established limits for biogenic amine content in meat (including poultry meat). Analyses of biogenic amine content in meat account for less than 10% of scientific papers, which raises the scope of the problem of limiting biogenic amines in meat. Among the methods of amine reduction are methods of destroying or reducing microorganisms’ high hydrostatic pressure (HHP), ozonisation, radiation, or the use of essential oils.