bluuebunny/crossref_metadata_2025 huggingface dataset:
Vaccine Breakthrough Infections by SARS-CoV-2 Variants after ChAdOx1 nCoV-19 Vaccination in Healthcare Workers
Authors: Kale Pratibha , Gupta Ekta , Bihari Chhagan , Patel Niharika , Rooge Sheetalnath , Pandey Amit , Bajpai Meenu , Khillan Vikas , Chattopadhyay Partha , Devi Priti , Maurya Ranjeet , Jha Neha , Mehta Priyanka , Kumar Manish , Sharma Pooja , Saifi Sheeba , Swaminathan Aparna , Alam Sarfaraz , Uppili Bharathram , Faruq Mohammed , Agrawal Anurag , Pandey Rajesh , Sarin Shiv Kumar
Published: January 2022

This study elucidated the clinical, humoral immune response and genomic analysis of vaccine breakthrough (VBT) infections after ChAdOx1 nCoV-19/Covishield vaccine in healthcare workers (HCWs). Amongst 1858 HCWs, 1639 had received either two doses (1346) or a single dose (293) of ChAdOx1 nCoV-19 vaccine. SARS-CoV-2 IgG antibodies and neutralizing antibodies were measured in the vaccinated group and the development of SARS-CoV-2 infection was monitored.Forty-six RT-PCR positive samples from the 203 positive samples were subjected to whole genome sequencing (WGS). Of the 203 (10.92%) infected HCWs, 21.46% (47/219) were non-vaccinated, which was significantly more than 9.52% (156/1639) who were vaccinated and infection was higher in doctors and nurses. Unvaccinated HCWs had 1.57 times higher risk compared to partially vaccinated HCWs and 2.49 times higher risk than those who were fully vaccinated.The partially vaccinated were at higher risk than the fully vaccinated (RR 1.58). Antibody non-response was seen in 3.44% (4/116), low antibody levels in 15.51% (18/116) and medium levels were found in 81.03% (94/116). Fully vaccinated HCWs had a higher antibody response at day 42 than those who were partially vaccinated (8.96 + 4.00 vs. 7.17 + 3.82). Whole genome sequencing of 46 samples revealed that the Delta variant (B.1.617.2) was predominant (69.5%). HCWs who had received two doses of vaccine showed better protection from mild, moderate, or severe infection, with a higher humoral immune response than those who had received a single dose. The genomic analysis revealed the predominance of the Delta variant (B.1.617.2) in the VBT infections.
Predictors of COVID-19 Vaccine Confidence: Findings from Slums of Four Major Metro Cities of India
Authors: Tamysetty Sathyanarayana , Babu Giridhara R. , Sahu Biswamitra , Shapeti Suresh , Ravi Deepa , Lobo Eunice , Varughese Chinnu Sara , Bhide Amita , Madhale Avinash , Manyal Mukta , Kamble Mahesh , Konar Asokananda , Sarkar Pabak , Das Dipesh Kumar , Mukherjee Partha Sarathi , Singh Kultar , Chauhan Ankur Singh , Naskar Aditya , Bhatia Rajesh , Khetrapal Sonalini
Published: January 2022

There are limited studies on COVID vaccine confidence at the household level in urban slums, which are at high risk of COVID-19 transmission due to overcrowding and poor living conditions. The objective was to understand the reasons influencing COVID-19 vaccine confidence, in terms of barriers and enablers faced by communities in urban slums and informal settlements in four major metro cities in India. A mixed method approach was adopted, where in field studies were conducted during April–May 2021. First, a survey of at least 50 subjects was conducted among residents of informal urban settlements who had not taken any dose of the COVID-19 vaccine in Mumbai, Bengaluru, Kolkata and Delhi; second, a short interview with five subjects who had taken at least one dose of the vaccine in each of the four cities to understand the factors that contributed to positive behaviour and, finally, an in-depth interview of at least 3 key informants in each city to ascertain the vaccination pattern in the communities. The reasons were grouped under contextual, individual/group and vaccine/vaccination specific issues. The most frequent reason (27.7%) was the uncertainty of getting the vaccine. The findings show the need for increasing effectiveness of awareness campaigns, accessibility and the convenience of vaccination, especially among vulnerable groups, to increase the uptake.
A Narrative Review of COVID-19 Vaccines
Authors: Eroglu Barbaros , Nuwarda Rina Fajri , Ramzan Iqbal , Kayser Veysel
Published: January 2022

The COVID-19 pandemic has shaken the world since early 2020 and its health, social, economic, and societal negative impacts at the global scale have been catastrophic. Since the early days of the pandemic, development of safe and effective vaccines was judged to be the best possible tool to minimize the effects of this pandemic. Drastic public health measures were put into place to stop the spread of the virus, with the hope that vaccines would be available soon. Thanks to the extraordinary commitments of many organizations and individuals from around the globe and the collaborative effort of many international scientists, vaccines against COVID-19 received regulatory approval for emergency human use in many jurisdictions in less than a year after the identification of the viral sequence. Several of these vaccines have been in use for some time; however, the pandemic is still ongoing and likely to persist for the foreseeable future. This is due to many reasons including reduced compliance with public health restrictions, limited vaccine manufacturing/distribution capacity, high rates of vaccine hesitancy, and the emergence of new variants with the capacity to spread more easily and to evade current vaccines. Here we discuss the discovery and availability of COVID-19 vaccines and evolving issues around mass vaccination programs.
COVID-19 Vaccination Acceptance among Healthcare Workers and General Population at the Very Beginning of the National Vaccination Program in Poland: A Cross-Sectional, Exploratory Study
Authors: Lindner-Pawłowicz Karolina , Mydlikowska-Śmigórska Agnieszka , Łampika Kamila , Sobieszczańska Małgorzata
Published: January 2022

The aim of the study was to assess the acceptance level of COVID-19 vaccination among healthcare workers (HCW) and the general population in Poland at the start of the national COVID-19 vaccination program from 18–31 December 2020. A cross-sectional anonymous survey was conducted in a group of 1976 people: 1042 health professionals and 934 non-medical professionals using an on-line questionnaire. The most skeptical about the COVID-19 vaccine were students of non-medical faculties, non-medical professions, and administrative–technical health service staff (26.2%, 38.7% and 41.2%, respectively). The most positive attitude to vaccination was reported by doctors, medical students and pharmacists (80.6%, 76.9% and 65.7%, respectively). Doctors (64.7%) and medical students (63.7%) most often declared confidence in vaccines compared to nurses (34.5%). Distrust about vaccine safety was declared by nurses (46.6%) and pharmacists (40.0%). HCW encouraged others to vaccinate more eagerly (65.8%) than non-medical professions (28.3%). Thus, a considerable proportion of HCW in Poland expressed concern about vaccines just prior to the beginning of the COVID-19 immunization program. The significant decrease in the willingness to vaccinate observed in Poland towards the end of 2021 must be considered in the light of the serious COVID-19 vaccination hesitancy in the Polish population.
Development of an Oral Salmonella-Based Vaccine Platform against SARS-CoV-2
Authors: Yoon Wonsuck , Park Yongsung , Kim Seunghyun , Bang Iel Soo
Published: January 2022

Effective vaccine development for global outbreaks, such as the coronavirus disease 2019 (COVID-19), has been successful in the short run. However, the currently available vaccines have been associated with a higher frequency of adverse effects compared with other general vaccines. In this study, the possibility of an oral bacteria-based vaccine that can be safely used as a platform for large-scale, long-term immunization was evaluated. A well-known Salmonella strain that was previously considered as a vaccine delivery candidate was used. Recombinant Salmonella cells expressing engineered viral proteins related with COVID-19 pathogenesis were engineered, and the formulation of the oral vaccine candidate strain was evaluated by in vitro and in vivo experiments. First, engineered S proteins were synthesized and cloned into expression vectors, which were than transformed into Salmonella cells. In addition, when orally administrated to mice, the vaccine promoted antigen-specific antibody production and cellular immunity was induced with no significant toxicity effects. These results suggest that Salmonella strains may represent a valuable platform for the development of an oral vaccine for COVID-19 as an alternative to tackle the outbreak of various mutated coronavirus strains and new infectious diseases in the future.
Design and Immunological Properties of the Novel Subunit Virus-like Vaccine against SARS-CoV-2
Authors: Krasilnikov Igor V. , Kudriavtsev Aleksandr V. , Vakhrusheva Anna V. , Frolova Maria E. , Ivanov Aleksandr V. , Stukova Marina A. , Romanovskaya-Romanko Ekaterina A. , Vasilyev Kirill A. , Mushenkova Nataliya V. , Isaev Artur A.
Published: January 2022

The COVID-19 pandemic is ongoing, and the need for safe and effective vaccines to prevent infection and to control spread of the virus remains urgent. Here, we report the development of a SARS-CoV-2 subunit vaccine candidate (Betuvax-CoV-2) based on RBD and SD1 domains of the spike (S) protein fused to a human IgG1 Fc fragment. The antigen is adsorbed on betulin adjuvant, forming spherical particles with a size of 100–180 nm, mimicking the size of viral particles. Here we confirm the potent immunostimulatory activity of betulin adjuvant, and demonstrate that two immunizations of mice with Betuvax-CoV-2 elicited high titers of RBD-specific antibodies. The candidate vaccine was also effective in stimulating a neutralizing antibody response and T cell immunity. The results indicate that Betuvax-CoV-2 has good potential for further development as an effective vaccine against SARS-CoV-2.
Antitumor Peptide-Based Vaccine in the Limelight
Authors: Kumai Takumi , Yamaki Hidekiyo , Kono Michihisa , Hayashi Ryusuke , Wakisaka Risa , Komatsuda Hiroki
Published: January 2022

The success of the immune checkpoint blockade has provided a proof of concept that immune cells are capable of attacking tumors in the clinic. However, clinical benefit is only observed in less than 20% of the patients due to the non-specific activation of immune cells by the immune checkpoint blockade. Developing tumor-specific immune responses is a challenging task that can be achieved by targeting tumor antigens to generate tumor-specific T-cell responses. The recent advancements in peptide-based immunotherapy have encouraged clinicians and patients who are struggling with cancer that is otherwise non-treatable with current therapeutics. By selecting appropriate epitopes from tumor antigens with suitable adjuvants, peptides can elicit robust antitumor responses in both mice and humans. Although recent experimental data and clinical trials suggest the potency of tumor reduction by peptide-based vaccines, earlier clinical trials based on the inadequate hypothesis have misled that peptide vaccines are not efficient in eliminating tumor cells. In this review, we highlighted the recent evidence that supports the rationale of peptide-based antitumor vaccines. We also discussed the strategies to select the optimal epitope for vaccines and the mechanism of how adjuvants increase the efficacy of this promising approach to treat cancer.
Immunogenicity of a Heterologous Prime-Boost COVID-19 Vaccination with mRNA and Inactivated Virus Vaccines Compared with Homologous Vaccination Strategy against SARS-CoV-2 Variants
Authors: Zhang Ruiqi , Liu Danlei , Leung Ka-Yi , Fan Yujing , Lu Lu , Chan Pui-Chun , To Kelvin Kai-Wang , Chen Honglin , Yuen Kwok-Yung , Chan Kwok-Hung , Hung Ivan Fan-Ngai
Published: January 2022

The emergence of SARS-CoV-2 variants may impact the effectiveness of vaccines, while heterologous vaccine strategy is considered to provide better protection. The immunogenicity of an mRNA-inactivated virus vaccine against the SARS-CoV-2 wild-type (WT) and variants was evaluated in the study. SARS-CoV-2 naïve adults (n = 123) were recruited and placed in the following groups: BNT162b2, CoronaVac or BNT162b2-CoronaVac (Combo) Group. Blood samples were collected to measure neutralization antibodies (NAb) by a live virus microneutralization assay (vMN) and surrogate NAb test. The day 56 vMN geometric mean titre (GMT) was 26.2 [95% confident interval (CI), [22.3–30.9] for Combo, 136.9 (95% CI, 104.2–179.7) for BNT162b2, and 14.7 (95% CI, 11.6–18.6) for CoronaVac groups. At 6 months post-first dose, the GMT declined to 8.0, 28.8 and 7.1 in the Combo, BNT162b2 and CoronaVac groups, respectively. Three groups showed reduced neutralizing activity against D614G, beta, theta and delta variants. At day 56 GMT (74.6) and month 6 GMT (22.7), the delta variant in the BNT162b2 group was higher than that in the Combo (day 56, 7.4; month 6, 5.5) and CoronaVac groups (day 56, 8.0; month 6, 5) (p < 0.0001). Furthermore, the mean surrogate NAb value on day 56 in the BNT162b2 group was 594.7 AU/mL and higher than 40.5 AU/mL in Combo and 38.8 AU/mL in CoronaVac groups (p < 0.0001). None of the participants developed severe adverse events, and all other adverse events were self-limiting. The Combo vaccination strategy was safe. The overall vaccine immunogenicity at day 56 and 6 months were comparable to the homologous CoronaVac group but inferior to the homologous BNT162b2 group, against both the WT and all variants. Furthermore, the antibody response of vaccines waned at 6 months and thereby, a third dose of the vaccine is needed for these vaccines.
Rapid Weight Loss, Central Obesity Improvement and Blood Glucose Reduction Are Associated with a Stronger Adaptive Immune Response Following COVID-19 mRNA Vaccine
Authors: Watanabe Mikiko , Balena Angela , Masi Davide , Tozzi Rossella , Risi Renata , Caputi Alessandra , Rossetti Rebecca , Spoltore Maria Elena , Biagi Filippo , Anastasi Emanuela , Angeloni Antonio , Mariani Stefania , Lubrano Carla , Tuccinardi Dario , Gnessi Lucio
Published: January 2022

Obesity is associated with a poor COVID-19 prognosis, and it seems associated with reduced humoral response to vaccination. Public health campaigns have advocated for weight loss in subjects with obesity, hoping to eliminate this risk. However, no evidence proves that weight loss leads to a better prognosis or a stronger immune response to vaccination. We aimed to investigate the impact of rapid weight loss on the adaptive immune response in subjects with morbid obesity. Twenty-one patients followed a hypocaloric, very-low-carbohydrate diet one week before to one week after the two mRNA vaccine doses. The diet’s safety and efficacy were assessed, and the adaptive humoral (anti-SARS CoV-2 S antibodies, Abs) and cell-mediated responses (IFNγ secretion on stimulation with two different SARS CoV-2 peptide mixes, IFNγ-1 and IFNγ-2) were evaluated. The patients lost ~10% of their body weight with metabolic improvement. A high baseline BMI correlated with a poor immune response (R −0.558, p = 0.013 for IFNγ-1; R −0.581, p = 0.009 for IFNγ-2; R −0.512, p = 0.018 for Abs). Furthermore, there was a correlation between weight loss and higher IFNγ-2 (R 0.471, p = 0.042), and between blood glucose reduction and higher IFNγ-1 (R 0.534, p = 0.019), maintained after weight loss and waist circumference reduction adjustment. Urate reduction correlated with higher Abs (R 0.552, p = 0.033). In conclusion, obesity is associated with a reduced adaptive response to a COVID-19 mRNA vaccine, and weight loss and metabolic improvement may reverse the effect.
The Chimeric Binjari-Zika Vaccine Provides Long-Term Protection against ZIKA Virus Challenge
Authors: Hazlewood Jessamine E. , Tang Bing , Yan Kexin , Rawle Daniel J. , Harrison Jessica J. , Hall Roy A. , Hobson-Peters Jody , Suhrbier Andreas
Published: January 2022

We recently developed a chimeric flavivirus vaccine technology based on the novel insect-specific Binjari virus (BinJV) and used this to generate a chimeric ZIKV vaccine (BinJ/ZIKA-prME) that protected IFNAR-/- dams and fetuses from infection. Herein, we show that a single vaccination of IFNAR-/- mice with unadjuvanted BinJ/ZIKA-prME generated neutralizing antibody responses that were retained for 14 months. At 15 months post vaccination, mice were also completely protected against detectable viremia and substantial body weight loss after challenge with ZIKVPRVABC59. BinJ/ZIKA-prME vaccination thus provided long-term protective immunity without the need for adjuvant or replication of the vaccine in the vaccine recipient, both attractive features for a ZIKV vaccine.
Factors That Impact Acceptance of COVID-19 Vaccination in Different Community-Dwelling Populations in China
Authors: Pan Jinhua , A Kezhong , Liu Zhixi , Zhang Peng , Xu Zhiyin , Guo Xiaoqin , Liu Guangtao , Xu Ao , Wang Jing , Wang Xinyu , Wang Weibing
Published: January 2022

(1) Background: It is important to improve vaccination strategies and immunization programs to achieve herd immunity to infectious diseases. (2) Methods: To assess the acceptance of COVID-19 vaccination, we conducted face-to-face surveys and online surveys in Shanghai, Zhejiang, and Qinghai provinces. A fixed-effect model and a random effects model were used to analyze factors associated with the acceptance of COVID-19 vaccination. (3) Findings: We initially recruited 3173 participants, 3172 participants completed the full questionnaire (the response rate was nearly 100%), of which 2169 were valid questionnaires, with an effective rate of 87.3%. The results indicated that 82.6% of participants were willing to receive vaccination when it was available in the community, and 57.2% of deliverymen, 43.3% of medical workers, 78.2% of parents of primary and secondary school children, and 72.2% of parents of preschool children were willing to receive vaccination. The models showed that participants who were male (female vs. male: OR = 1.49, 95% CI (1.12, 1.98)), 60 to 69 years-old (60–69 vs. <30: OR = 0.52, 95% CI (0.29, 0.92)), had less education (medium vs. low: OR = 1.50, 95% CI (1.05, 2.23)), had good health status (good vs. low: OR = 0.36, 95% CI (0.15, 0.88)), and had positive attitudes and trust (OR = 0.14, 95% CI (0.10, 0.20)) in vaccines approved by the National Health Commission were more likely to accept vaccination. Participants also had an increased vaccination acceptance if it was recommended by government sources, doctors, relatives, or friends. Most participants learned about COVID-19 vaccination from television, radio, and newspapers, followed by community or hospital campaigns and the internet. (4) Conclusions: Government sources and doctors could increase the acceptance of vaccination by promoting the efficacy and safety of COVID-19 vaccination by the use of mass media and emphasizing the necessity of vaccination for everyone.
Safety and Immunogenicity of Standard and Double Doses of Hepatitis B Vaccine in Children after Liver Transplantation: An Open-Label, Randomised Controlled Trial
Authors: Sintusek Palittiya , Buranapraditkun Supranee , Wanawongsawad Piyaporn , Posuwan Nawarat , Thantiworasit Pattarawat , Wanlapakorn Nasamon , Klaewsongkram Jettanong , Suratannon Narissara , Chaijitraruch Nataruks , Chongsrisawat Voranush , Poovorawan Yong
Published: January 2022

A high prevalence of hepatitis B (HepB) antibody loss after liver transplantation (LT) and de novo HepB infection (DNH) was documented, hence revaccination to prevent DNH is crucial. This study aimed to compare the safety and immunogenicity of two HepB vaccine regimens in liver-transplanted children. Liver-transplanted children who were previously immunised but showed HepB surface antibodies (anti-HBs) ≤ 100 mIU/mL were randomised to receive a standard three-dose (SD) and double three-dose (DD) vaccine intramuscularly in months 0–1–6. Anti-HBs and T-cell-specific response to the HepB antigen were assessed. A total of 61 children (54.1% male, aged 1.32 ± 1.02 years) completed the study without any serious adverse reaction. The seroprotective rate was 69.6% vs. 60% (p = 0.368) and 91.3% vs. 85% (p = 0.431) in SD and DD after the first and third 3-dose vaccinations, respectively. The geometric mean titre (95% confidence interval) of anti-HBs in SD and DD were 443.33 (200.75–979.07) vs. 446.17 (155.58–1279.50) mIU/mL, respectively, at completion. Numbers of interferon-γ-secreting cells were higher in hyporesponders/responders than in nonresponders (p = 0.003). The significant factors for the immunologic response to HepB vaccination were anti-HB levels prevaccination, tacrolimus trough levels, and time from LT to revaccination. SD and DD had comparative immunogenicity and were safe for liver-transplanted children who were previously immunised.
Initial Mix-and-Match COVID-19 Vaccination Perceptions, Concerns, and Side Effects across Canadians
Authors: Palanica Adam , Jeon Jouhyun
Published: January 2022

Research indicates that mixing the first two doses of COVID-19 vaccine types (i.e., adenoviral vector and mRNA) produces potent immune responses against the coronavirus, but it is unclear how individuals may perceive these benefits, or whether there are different concerns compared to individuals who received two doses of the same vaccine. This research examines the demographic characteristics, psychological perceptions, and vaccination-related opinions and experiences of a large Canadian sample (N = 1002) who had received two initial doses of any COVID-19 vaccine combination. Participants included 791 (78.9%) who received two doses of the exact same brand and type of vaccine, 164 (16.4%) who received two doses of the same type of vaccine (i.e., either mRNA or adenoviral vector) but from different brands (e.g., Pfizer-BioNTech + Moderna), and 47 (4.7%) who received two doses from different types and brands of vaccine (e.g., Oxford-AstraZeneca + Pfizer-BioNTech). Results showed that, after the first vaccine dose, participants who received an adenoviral vector vaccine (e.g., Oxford-AstraZeneca) experienced the highest number of common side effects, and more severe levels of each side effect compared to those who received an mRNA vaccine (e.g., Pfizer-BioNTech or Moderna). After the second dose, participants who received Moderna as their second vaccine experienced the highest number of and most severe side effects, regardless of whether they received Moderna, Pfizer-BioNTech, or Oxford-AstraZeneca as their first dose. Real-world implications of these findings are discussed.
Efficacy of High-Dose Polyclonal Intravenous Immunoglobulin in COVID-19: A Systematic Review
Authors: Focosi Daniele , Franchini Massimo , Tuccori Marco , Cruciani Mario
Published: January 2022

Background: Although several therapeutic strategies have been investigated, the optimal treatment approach for patients with coronavirus disease (COVID-19) remains to be elucidated. This systematic review and meta-analysis aimed to evaluate the efficacy and safety of polyclonal intravenous immunoglobulin (IVIG) therapy in COVID-19. Methods: A systematic literature search using appropriate medical subject heading (MeSH) terms was performed through Medline (PubMed), EMBASE, SCOPUS, OVID and Cochrane Library electronic databases. The main outcomes considered were mortality and safety of IVIG versus placebo/standard of care. This review was carried out in accordance with Cochrane methodology including the risk bias assessment and grading of the quality of evidence. Measures of treatment effect were mean differences (MD) together with 95% confidence intervals (CIs) for continuous outcome measures and risk ratio (RR) or MD for binary outcomes. Two reviewers independently extracted data from individual studies, and disagreements were resolved by a third reviewer. Results: A total of 2401 COVID-19 patients from 10 studies (four randomized controlled trials (RCT) and six non-randomized controlled trials (non-RCTs)) were included in the analysis. Participants received IVIG or placebo/standard of care. The use of IVIG was not associated with a significantly reduced risk of death (RR 0.50, 95% CIs 0.18–1.36, p = 0.17 for RCTs; RR 0.95, 95% CIs 0.61–1.58, p = 0.94 for non-RCTs; low certainty of evidence). IVIG significantly reduced the length of hospital stay (MD −2.24, 95% CIs −3.20/−1.27; p = 0.00001; low certainty of evidence), although this difference was significant only for studies evaluating moderate COVID-19 patients. No significant difference was observed in the incidence of overall and serious adverse events between IVIG recipients and controls (very low certainty of evidence). Conclusions: The current evidence from the literature does not support the use of IVIG in COVID-19 patients.
Comparative Immunogenicity of the Recombinant Receptor-Binding Domain of Protein S SARS-CoV-2 Obtained in Prokaryotic and Mammalian Expression Systems
Authors: Merkuleva Iuliia A. , Shcherbakov Dmitry N. , Borgoyakova Mariya B. , Shanshin Daniil V. , Rudometov Andrey P. , Karpenko Larisa I. , Belenkaya Svetlana V. , Isaeva Anastasiya A. , Nesmeyanova Valentina S. , Kazachinskaia Elena I. , Volosnikova Ekaterina A. , Esina Tatiana I. , Zaykovskaya Anna V. , Pyankov Oleg V. , Borisevich Sophia S. , Shelemba Arseniya A. , Chikaev Anton N. , Ilyichev Alexander A.
Published: January 2022

The receptor-binding domain (RBD) of the protein S SARS-CoV-2 is considered to be one of the appealing targets for developing a vaccine against COVID-19. The choice of an expression system is essential when developing subunit vaccines, as it ensures the effective synthesis of the correctly folded target protein, and maintains its antigenic and immunogenic properties. Here, we describe the production of a recombinant RBD protein using prokaryotic (pRBD) and mammalian (mRBD) expression systems, and compare the immunogenicity of prokaryotic and mammalian-expressed RBD using a BALB/c mice model. An analysis of the sera from mice immunized with both variants of the protein revealed that the mRBD expressed in CHO cells provides a significantly stronger humoral immune response compared with the RBD expressed in E.coli cells. A specific antibody titer of sera from mice immunized with mRBD was ten-fold higher than the sera from the mice that received pRBD in ELISA, and about 100-fold higher in a neutralization test. The data obtained suggests that mRBD is capable of inducing neutralizing antibodies against SARS-CoV-2.
Comparison of Feed Digestibility between Ponies, Standardbreds and Andalusian Horses Fed Three Different Diets
Authors: Potter Samantha , Bamford Nicholas , Baskerville Courtnay , Harris Patricia , Bailey Simon
Published: January 2022

Ponies and some horse breeds such as Andalusians exhibit an ‘easy keeper’ phenotype and tend to become obese more readily than other breeds such as Standardbreds. Various hypotheses have been proposed, including differences in appetite or metabolic efficiency. This study aimed to investigate the effect of breed on nutrient digestibility. Ponies, Standardbreds and Andalusian horses were adapted to consuming either a control fibre-based diet (n = 9), a hypercaloric cereal-rich diet (n = 12) or a hypercaloric fat-rich diet (n = 12) over 20 weeks. Total faecal collection was performed over 24 h to determine apparent total tract digestibility of gross energy, dry matter (DM), neutral detergent fibre (NDF), starch, crude protein and crude fat. There was no effect of breed on apparent digestibility for any of the nutrients studied (all p > 0.05). However, there was a significant effect of diet, with animals consuming the cereal-rich or fat-rich diets demonstrating higher digestibility of gross energy, DM, NDF and crude protein compared with those consuming the control diet (all p < 0.05). Animals adapted to the cereal-rich diet demonstrated higher digestibility of starch (p < 0.001) and animals adapted to the fat-rich diet demonstrated higher digestibility of fat (p < 0.001). This study found that horses and ponies had similar nutrient digestibility when adapted to the same diets and management conditions. Limitations included the relatively small number of animals from each breed per diet group and the short period of total faecal collection. The tendency towards increased adiposity in ponies and Andalusian-type horse breeds is more likely to reflect differences in metabolism, rather than differences in feed digestibility.
Groundwater Radon Precursor Anomalies Identification by EMD-LSTM Model
Authors: Feng Xiaobo , Zhong Jun , Yan Rui , Zhou Zhihua , Tian Lei , Zhao Jing , Yuan Zhengyi
Published: January 2022

Groundwater radon concentrations can reflect the changes of crustal stress and strain. Scholars and scientific institutions have also recorded groundwater radon precursor anomalies before earthquakes. Therefore, groundwater radon monitoring is an effective means of predicting seismic activities. However, the variation of radon concentrations within groundwater is not only affected by structural factors, but also by environmental factors, such as air pressure, temperature, and rainfall. This causes difficulty in identifying the possible precursor anomalies. Therefore, the EMD-LSTM model is proposed to identify the radon anomalies. This study investigated the time series data of groundwater radon from well #32 located in Sichuan province. Three models (including the LSTM (Long Short-Term Memory) model with auxiliary data, the EMD-LSTM (Empirical Mode Decomposition Long Short-Term Memory) model with auxiliary data, and the EMD-LSTM model without auxiliary data) were developed in order to predict groundwater radon variations. The results indicated that the prediction accuracy of the EMD-LSTM model was much higher than that of the LSTM model, and the EMD-LSTM model without auxiliary data also can obtain an ideal prediction result. Furthermore, the different durations of seismic activities T (T = ±10, ±30, ±50, and ±100) were also investigated by comparing the identification results. The identification rate of the precursor anomalies was the highest when T = ±30. The EMD-LSTM model identified five possible radon anomalies among the seven selected earthquakes. Taking well #32 as an example, we provided a promising method, that was the EMD-LSTM model, to detect the groundwater radon anomalies. It also suggested that the EMD-LSTM model can be used to identify the possible precursor anomalies within future studies.
Proximity to Riparian Wetlands Increases Mercury Burden in Fish in the Upper St. Lawrence River
Authors: Osgood Autumn , Brahmstedt Evie S. , Windle Matthew J.S. , Holsen Thomas M. , Twiss Michael R.
Published: January 2022

Mercury deposited in the Upper St. Lawrence River watershed by atmospheric deposition accumulated in riparian wetlands and is at risk of remobilization due to water level fluctuations. To examine if riparian wetlands are a source of mercury to fish, 174 yellow perch (Perca flavescens) and 145 round gobies (Neogobius melanostomus) were collected in 2019 from eight wetland and seven non-wetland habitats throughout the Upper St. Lawrence River. Mercury levels were significantly (p < 0.01) higher in fish collected from wetlands than those collected from non-wetland habitats for both yellow perch and round goby. Perch had mercury concentrations of 74.5 ± 35.4 ng/g dry wt in wetlands compared to 59.9 ± 23.0 ng/g dry wt in non-wetlands. Goby had mercury concentrations of 55.4 ± 13.8 ng/g dry wt in wetlands and non-wetland concentrations of 41.0 ± 14.0 ng/g dry wt. Riparian wetlands are areas of elevated mercury methylation and mobilization in the Upper St. Lawrence River and consequences to predators should be considered from the perspective of both wildlife preservation as well as fish consumption advisories for public health concerns.
Object-Based Multigrained Cascade Forest Method for Wetland Classification Using Sentinel-2 and Radarsat-2 Imagery
Authors: Liu Huaxin , Jiang Qigang , Ma Yue , Yang Qian , Shi Pengfei , Zhang Sen , Tan Yang , Xi Jing , Zhang Yibo , Liu Bin , Gao Xin
Published: January 2022

The development of advanced and efficient methods for mapping and monitoring wetland regions is essential for wetland resources conservation, management, and sustainable development. Although remote sensing technology has been widely used for detecting wetlands information, it remains a challenge for wetlands classification due to the extremely complex spatial patterns and fuzzy boundaries. This study aims to implement a comprehensive and effective classification scheme for wetland land covers. To achieve this goal, a novel object-based multigrained cascade forest (OGCF) method with multisensor data (including Sentinel-2 and Radarsat-2 remote sensing imagery) was proposed to classify the wetlands and their adjacent land cover classes in the wetland National Natural Reserve. Moreover, a hybrid selection method (ReliefF-RF) was proposed to optimize the feature set in which the spectral and polarimetric decomposition features are contained. We obtained six spectral features from visible and shortwave infrared bands and 10 polarimetric decomposition features from the H/A/Alpha, Pauli, and Krogager decomposition methods. The experimental results showed that the OGCF method with multisource features for land cover classification in wetland regions achieved the overall accuracy and kappa coefficient of 88.20% and 0.86, respectively, which outperformed the support vector machine (SVM), extreme gradient boosting (XGBoost), random forest (RF), and deep neural network (DNN). The accuracy of the wetland classes ranged from 75.00% to 97.53%. The proposed OGCF method exhibits a good application potential for wetland land cover classification. The classification scheme in this study will make a positive contribution to wetland inventory and monitoring and be able to provide technical support for protecting and developing natural resources.
Influence of Particle Size of River Sand on the Decontamination Process in the Slow Sand Filter Treatment of Micro-Polluted Water
Authors: Ji Xuemei , Zhao Cui , Lv Yufeng , Yang Jifu , Li Bin
Published: January 2022

Slow sand filters (SSFs) have been widely used in the construction of water plants in rural areas. It is necessary to find river sand of suitable particle size to improve SSF treatment of micro-polluted water so as to ensure the effective and long-term operation of these plants. In this study, SSF1# (particle size of 0.1–0.5 mm), SSF2# (particle size of 0.5–1 mm), and SSF3# (particle size of 1–1.5 mm) were selected. The physical absorption, CODMn and NH4+-N removal effect, and microbial community were analyzed. According to Langmuir and Freundlich adsorption model fitting, the smaller the particle size of the river sand, the more pollutants are adsorbed under the same conditions. SSF1# has the shortest membrane-forming time, highest CODMn and NH4+-N removal rate, and highest Shannon estimator, indicating that there are more abundant microbial species in the biofilm. Mesorhizobium, Pannonibacter, Pseudoxanthomonas, Aquabacterium, Devosia, and other bacteria have different proportions in each system, each forming its own stable biological chain system. The effluent quality of the three SSFs can meet drinking water standards. However, river sand with a particle size range of 0.1–0.5 mm is easily blocked, and thus the recommended size range for SSF is 0.5–1 mm.
Inka Hydraulic Engineering at the Tipon Royal Compound (Peru)
Authors: Ortloff Charles R.
Published: January 2022

The Inka site of Tipon had many unique hydraulic engineering features that have modern hydraulic theory counterparts. For example, the Tipon channel system providing water to the Principal Fountain had a channel contraction inducing critical flow as determined by CFD analysis- this feature designed to induce flow stability and preserve the aesthetic display of the downstream Waterfall. The Main Aqueduct channel sourced by the Pukara River had a given flow rate to limit channel overbank spillage induced by a hydraulic jump at the steep-mild slope transition channel location as determined by use of modern CFD methods- this flow rate corresponds to the duplication of the actual flow rate used in the modern restoration using flow blockage plates placed in the channel to limit over-bank spillage. Additional hydraulic features governing the water supply to agricultural terraces for specialty crops constitute further sophisticated water management control systems discussed in detail in the text.
A SEEC Model Based on the DPSIR Framework Approach for Watershed Ecological Security Risk Assessment: A Case Study in Northwest China
Authors: Wang Bin , Yu Fang , Teng Yanguo , Cao Guozhi , Zhao Dan , Zhao Mingyan
Published: January 2022

The DPSIR model is a conceptual model established by the European Environment Agency to solve environmental problems. It provides an overall framework for analysis of environmental problems from five aspects: driving force (D), pressure (P), state (S), impact (I), and response (R). Through use of the DPSIR model framework, this paper presents the SEEC model approach for evaluating watershed ecological security. The SEEC model considers four aspects: socioeconomic impact (S), ecological health (E), ecosystem services function (E), and control management (C). Through screening, 38 evaluation indicators of the SEEC model were determined. The evaluation results showed that the ecological security index of the study area was >80, indicating a generally safe level. The lowest score was mainly attributable to the low rate of treatment of rural domestic sewage. The water quality status was used to evaluate the applicability of the SEEC model, and the calculation results indicated that the higher the score of the ecological security evaluation results, the better the water quality status. The findings show that the SEEC model demonstrates satisfactory applicability to evaluation of watershed ecological security.
A First Glimpse on Cold-Adapted PCB-Oxidizing Bacteria in Edmonson Point Lakes (Northern Victoria Land, Antarctica)
Authors: Papale Maria , Lo Giudice Angelina , Rappazzo Alessandro Ciro , Azzaro Maurizio , Rizzo Carmen
Published: January 2022

Antarctic freshwater ecosystems are especially vulnerable to human impacts. Polychlorobiphenyls (PCBs) are persistent organic pollutants that have a long lifetime in the environment. Despite their use having either been phased out or restricted, they are still found in nature, also in remote areas. Once in the environment, the fate of PCBs is strictly linked to bacteria which represent the first step in the transfer of toxic compounds to higher trophic levels. Data on PCB-oxidizing bacteria from polar areas are still scarce and fragmented. In this study, the occurrence of PCB-oxidizing cold-adapted bacteria was evaluated in water and sediment of four coastal lakes at Edmonson Point (Northern Victoria Land, Antarctica). After enrichment with biphenyl, 192 isolates were obtained with 57 of them that were able to grow in the presence of the PCB mixture Aroclor 1242, as the sole carbon source. The catabolic gene bphA, as a proxy for PCB degradation potential, was harbored by 37 isolates (out of 57), mainly affiliated to the genera Salinibacterium, Arthrobacter (among Actinobacteria) and Pusillimonas (among Betaproteobacteria). Obtained results enlarge our current knowledge on cold-adapted PCB-oxidizing bacteria and pose the basis for their potential application as a valuable eco-friendly tool for the recovery of PCB-contaminated cold sites.
Four Years of Sediment and Phosphorus Monitoring in the Kraichbach River Using Large-Volume Samplers
Authors: Allion Katharina , Kiemle Lisa , Fuchs Stephan
Published: January 2022

Various sampling strategies come into operation to monitor water quality in rivers. Most frequently, grab samples are taken, but they are not suitable for recording the highly dynamic transport of solids and solid-bound pollutants. Composite samples reduce the influence of input and transport dynamics and are better suited to determine the annual river loads. Large-volume samplers (LVSs) produce both a composite sample over a long period of time and an amount of solids which allows for further analyses. In the small sub-catchment area of the Kraichbach river in Baden-Wuerttemberg (Germany) two LVSs have been installed to sample the river flow. The concentration of solids and phosphorus in the supernatant water and the settled sediment in the sampler have been determined and mean concentrations have been derived. Annual river loads were calculated in combination with discharge data from the nearby gauging station. Two sampling strategies of the LVS were tested and compared. For the first strategy, the LVS was used to collect quasi-continuous composite samples throughout the year, whereas, with the second strategy, samples were taken specifically for different flow conditions (low, mean and high flow). This study compares the advantages and constraints of both strategies. Results indicate that the first strategy is better suited to determine annual river loads. Quasi-continuous large-volume composite sampling is recommended for further monitoring campaigns.
Financial and Economic Investment Evaluation of Wastewater Treatment Plant
Authors: Ćetković Jasmina , Knežević Miloš , Lakić Slobodan , Žarković Miloš , Vujadinović Radoje , Živković Angelina , Cvijović Jelena
Published: January 2022

Improved Cost-Benefit Analysis (CBA) analysis requires a broader analytical framework, in order to perceive each project individually from the perspective of potentially measurable and significant effects on the environment and society as a whole. The main goal of our paper is to assess the financial and economic justification for variant V3 (as the most technically optimal) of the wastewater treatment plant (WWTP) construction project in Nov Dojran, North Macedonia, with the purpose of advancing municipal infrastructure and environmental benefits from improved water treatment. Based on the economic analysis conducted, we conclude that the investment in the WWTP project is justified, because the economic internal rate of return is higher than the opportunity cost of capital (EIRR = 16.38%), the economic net present value is higher than 0, and EBCR (benefit-cost ratio) is greater than 1 (EBCR = 2.11). The highest environmental benefit of 49.2% in total environmental benefits is associated with nitrogen, while phosphorus is the next pollutant in the structure of environmental benefits at 46.1%. The environmental benefits of removing biological oxygen demand (BOD) and chemical oxygen demand (COD) are significantly less important, despite the removal of significant amounts of these pollutants during treatment. The situation is similar with suspended particles.
Degradation of Azo Dyes with Different Functional Groups in Simulated Wastewater by Electrocoagulation
Authors: Liu Yang , Li Chenglong , Bao Jia , Wang Xin , Yu Wenjing , Shao Lixin
Published: January 2022

Increasing attention has been paid to the widespread contamination of azo dyes in water bodies globally. These chemicals can present high toxicity, possibly causing severe irritation of the respiratory tract and even carcinogenic effects. The present study focuses on the periodically reverse electrocoagulation (PREC) treatment of two typical azo dyes with different functional groups, involving methyl orange (MO) and alizarin yellow (AY), using Fe-Fe electrodes. Based upon the comparative analysis of three main parameters, including current intensity, pH, and electrolyte, the optimal color removal rates for MO and AY could be achieved at a rate of up to 98.7% and 98.6%, respectively, when the current intensity is set to 0.6 A, the pH is set at 6.0, and the electrolyte is selected as NaCl. An accurate predicted method of response surface methodology (RSM) was established to optimize the PREC process involving the three parameters above. The reaction time was the main influence for both azo dyes, while the condition of PREC treatment for AY simulated wastewater was time-saving and energy conserving. According to the further UV–Vis spectrophotometry analysis throughout the procedure of the PREC process, the removal efficiency for AY was better than that of MO, potentially because hydroxyl groups might donate electrons to iron flocs or electrolyze out hydroxyl free radicals. The present study revealed that the functional groups might pose a vital influence on the removal efficiencies of the PREC treatment for those two azo dyes.
Identification of Hydrodynamic Dispersion Tensor by Optimization Algorithm Based LBM/CMA-ES Combination
Authors: Smaoui Hassan , Zouhri Lahcen , Kaidi Sami
Published: January 2022

The hydrodynamic dispersion tensor (HDT) of a porous medium is a key parameter in engineering and environmental sciences. Its knowledge allows for example, to accurately predict the propagation of a pollution front induced by a surface (or subsurface) flow. This paper proposes a new mathematical model based on inverse problem-solving techniques to identify the HDT (noted D=) of the studied porous medium. We then showed that in practice, this new model can be written in the form of an integrated optimization algorithm (IOA). The IOA is based on the numerical solution of the direct problem (which solves the convection–diffusion type transport equation) and the optimization of the error function between the simulated concentration field and that observed at the application site. The partial differential equations of the direct model were solved by high resolution of (Δx=Δy=1 m) Lattice Boltzmann Method (LBM) whose computational code is named HYDRODISP-LBM (HYDRO-DISpersion by LBM). As for the optimization step, we opted for the CMA-ES (Covariance Matrix Adaptation-Evolution Strategy) algorithm. Our choice for these two methods was motivated by their excellent performance proven in the abundant literature. The paper describes in detail the operation of the coupling of the two computer codes forming the IOA that we have named HYDRODISP-LBM/CMA-ES. Finally, the IOA was applied at the Beauvais experimental site to identify the HDT D=. The geological analyzes of this site showed that the tensor identified by the IOA is in perfect agreement with the characteristics of the geological formation of the site which are connected with the mixing processes of the latter.
Estimation of Groundwater Depletion in Iran’s Catchments Using Well Data
Authors: Safdari Zohreh , Nahavandchi Hossein , Joodaki Gholamreza
Published: January 2022

Iran is experiencing significant water challenges that have now turned water security into a national priority. By estimating secular trend groundwater storage in Iran between 2002 and 2017, we see that there is an intensive negative trend, even −4400 Mm3 in some areas. These estimations show shifting in the climate and extra extraction from aquifers for agricultural use in some areas in Iran. The secular trend of groundwater storage changes across the whole of Iran inferred from observation well data is −20.08 GT/yr. The secular trends of GWS changes based on observation well data are: −11.55 GT/yr for the Central Plateau basin, −3.60 GT/yr for the Caspian Sea basin, −3.0 GT/yr for the Persian Gulf and Oman Sea basin, −0.53 GT/yr for the Urmieh Lake basin, −0.57 GT/yr for the Eastern Boundary basin, and −0.83 GT/yr for the Gharaghom basin. The most depleted sub-basin (Kavir Markazi) has secular trends of GWS changes of −4.503 GT/yr. This study suggests that groundwater depletion is the largest single contributor to the observed negative trend of groundwater storage changes in Iran, the majority of which occurred after the drought in 2007. The groundwater loss that has been accrued during the study period is particularly alarming for Iran, which is already facing severe water scarcity.