bluuebunny/crossref_metadata_2025 huggingface dataset:
Training Plan for the Continuity of Non-Presential Education in Six Peruvian Universities during COVID-19
Authors: Pérez-Sánchez Lourdes , Lavandera-Ponce Silvia , Mora-Jaureguialde Begoña , Martín-Cuadrado Ana María
Published: January 2022

This article shows the response offered by the UTEC-UNED-TECSUP Consortium to six Peruvian public (national) universities aimed at strengthening the digital competences of their communities, made up of managers, teachers, students, and support technicians. The contextual and situational diagnosis, which covered organizational, technological, and competence dimensions, revealed a series of cross-cutting needs related to technological skills that prevented the training or mobilization of the digital competences necessary for progress in the other dimensions under study. The response was an online training plan, consisting of three training programs and eighty-three courses. The pedagogical strategy was based on the scaling of competence achievements that ended with problem-solving and applications in daily activities. The accompaniment was carried out through virtual tutorials, distributed via synchronous and asynchronous sessions. In total, 5034 were involved—347 were teachers and managers, 4932 were students, and 25 were technical staff. The pedagogical and socio-emotional limitations of the university community, as well as the scarcity of technological resources and poor connectivity, meant that the plan was only partially implemented. Moreover, the short and intense period of development to which the universities were subjected was also a factor.
A Scoping Review on the Prevalence and Determinants of Post-Traumatic Stress Disorder among Military Personnel and Firefighters: Implications for Public Policy and Practice
Authors: Obuobi-Donkor Gloria , Oluwasina Folajinmi , Nkire Nnamdi , Agyapong Vincent I.O.
Published: January 2022

Introduction: Firefighters and military personnel are public safety personnel who protect the safety of individuals and their properties. They are usually exposed to traumatic events leaving them at risk of developing mental health conditions such as post-traumatic stress disorder (PTSD). Increasing concern is being raised regarding the mental health impacts, specifically PTSD, among military personnel and firefighters. Objective: There is an increased exposure of firefighters and military personnel to traumatic events and the attendant risk of developing post-traumatic stress disorder. It is crucial to ascertain the level of PTSD amongst this cohort and determinants to formulate policies and practices that mitigate the risk and protect public safety personnel. This scoping review sought to determine the prevalence of PTSD among this cohort globally and to explore determinants of this mental health condition. Methods: A literature search in databases including MEDLINE, CINAHL, PubMed, PsycINFO, and EMBASE was conducted electronically from May 2021 to 31 July 2021. Two reviewers independently assessed full-text articles according to the predefined inclusion criteria and screening process undertaken to identify studies for the review. Articles were screened with a third reviewer, resolving conflicts where necessary and further assessing them for eligibility. During article selection, the PRISMA checklist was adopted, and with the Covidence software, a total of 32 articles were selected for the final examination. For the eligible studies, data extraction was conducted, information was collated and summarized, and the findings were reported. Original qualitative and quantitative data on the prevalence and predictors of PTSD among veterans, military, and firefighters were reported. Results: The prevalence of PTSD was 57% for firefighters and 37.8% for military personnel. Demographic factors, job factors, social support, injuries, physical and psychological factors, and individual traits were the main predictors of PTSD in this cohort. Conclusion: This information is vital for developing and implementing prevention and intervention strategies for PTSD in military personnel and firefighters. Recognizing and addressing factors that predict PTSD will help to improve mental wellbeing and increase productivity. More peer-reviewed studies are needed on the prevalence of PTSD amongst these cohorts.
How Does the COVID-19 Pandemic Affect People’s Willingness to Pay for Health in the Short and Long Term? A Longitudinal Study during and after the COVID-19 Pandemic in China
Authors: Song Wei , Zhao Taiyang , Huang Ershuai
Published: January 2022

The COVID-19 pandemic has posed a substantial threat to people’s lives and aroused health concerns. This study aims at exploring the following questions. First, how does the COVID-19 pandemic affect people’s willingness to pay for health (WPH) in the short and long term? Second, what is the psychological mechanism underlying such an effect? Finally, what are the boundary conditions for this effect? To answer these questions, we conducted three longitudinal surveys. The first survey was launched in February 2020—the time of the most serious outbreak of COVID-19 in China. Data were obtained from 1548 participants through questionnaires on an online survey platform. The sample covered 297 prefecture-level cities in 31 provincial administrative regions. Subsequently, we conducted two follow-up surveys in August 2020 and July 2021. The samples of these surveys were randomly selected from the sample of the first survey. The findings showed that the pandemic promoted people’s WPH in the outbreak period. The fear of death and self-esteem mediated and moderated this effect, respectively. Moreover, the effect persisted for six months after the COVID-19 pandemic had been brought under control (August 2020). However, the effect disappeared after a year and a half (July 2021). These results indicate that the COVID-19 pandemic promoted people’s WPH and that this effect was sustained in the short term after the pandemic had been brought under control but not in the long term.
Cervical Myofascial Pain Is Associated with an Imbalance of Masticatory Muscle Activity
Authors: Ginszt Michał , Szkutnik Jacek , Zieliński Grzegorz , Bakalczuk Magdalena , Stodółkiewicz Małgorzata , Litko-Rola Monika , Ginszt Apolinary , Rahnama Mansur , Majcher Piotr
Published: January 2022

This study aimed to assess the relationship between the occurrence of cervical myofascial pain with active myofascial trigger points (MTrPs) within the upper trapezius muscle and the electromyographic asymmetry index (AsI) of masticatory muscles: temporalis anterior (TA), superficial part of the masseter muscle (MM), and anterior belly of the digastric muscle (DA). The study group comprised 100 subjects (80 women and 20 men) aged 18 to 30 years (mean 23 ± 2.6 years) reporting pain in the neck muscles, diagnosed with myofascial pain with active MTrPs only within the upper trapezius muscle. The control group comprised 60 healthy, pain-free subjects (42 women and 18 men) aged 20 to 30 years (mean 22.8 ± 2.6 years) without MTrPs in the upper trapezius muscle. The palpation measurement, based on the diagnostic criteria of Travell and Simons, was used to diagnose active MTrPs. The masticatory muscle activity was recorded using an 8-channel device for surface electromyography—BioEMG IIITM. Significant differences in electromyographic patterns between the group with MTrPs in the right side of upper trapezius muscle and the control group were observed within resting activity for the AsI TA (MTrPs: 8.64 vs. controls: −3.22; p = 0.001) and AsI MM (MTrPs: 7.05 vs. controls: −2.09; p = 0.018). Controls presented different electromyographic patterns during maximum voluntary clenching with cotton rolls between teeth within masseter muscle compared to the MTrPs group (MTrPs: 9.27 vs. controls: −0.43 vs. p = 0.041). Participants with MTrPs in the left side of upper trapezius muscle presented predomination of left-sided electromyographic patterns at rest within temporalis anterior in comparison to controls (MTrPs: −19.22 vs. controls: −3.22; p = 0.001). MTrPs within the trapezius muscle may be related to asymmetry within the masticatory muscle activity, suggesting that the presence of myofascial pain within the cervical muscles plays a role in the imbalance of the stomatognathic system. A unilateral active MTrPs within the trapezius muscle may increase the sEMG activity on the same side of the temporalis anterior and masseter muscles.
Body Composition and Physical Fitness: Does This Relationship Change in 4 Years in Young Adults?
Authors: Kochman Maciej , Kasperek Wojciech , Guzik Agnieszka , Drużbicki Mariusz
Published: January 2022

(1) Background: There are few studies investigating the relationship between physical fitness and body composition in young adults and, to our knowledge, there are no such reports focusing specifically on physiotherapy students. This observational study aimed to assess the relationship between physical fitness and selected anthropometric measurements as well as body composition in Polish students in the first year and in the final year of a university course in physiotherapy. (2) Methods: A group of 100 randomly selected individuals were recruited among first- and fifth-year students of physiotherapy (50% women), who were assigned to two groups: A (aged 19 years) and B (aged 23 years). Body composition was assessed using a Tanita TBF-300 Analyzer, and physical fitness was measured using Zuchora’s Physical Fitness Index. (3) Results: A higher level of general fitness was identified in students from Group B (p = 0.0261), and lower values of the fat mass index was found in Group A (p = 0.0441). Group A was found with correlations between general level of physical fitness and the following indexes: fat% (R = −0.4; p = 0.0018), FM (R = −0.3; p = 0.0310), FFM (R = 0.3; p = 0.0229) and TBW (R = 0.4; p = 0.0024), whereas Group B was found with correlations between general physical fitness and BMI (R = 0.3; p = 0.0308) as well as FM index (R = 0.3; p = 0.0431). (4) Conclusions: The findings show significant differences between the groups in physical fitness, body composition and selected anthropometric measurements. Older students presented higher level of general fitness, whereas younger students were found with a lower mean value of fat index.
Trends and Causes of Raw Water Quality Indicators in the Five Most Famous Lakes of Jiangsu Province, China
Authors: Chang Yajun , Feng Zheyuan , Liu Jixiang , Sun Junfang , Sun Linhe , Tang Qiang , Yao Dongrui
Published: January 2022

Due to pollutants from industrial and agricultural activities, the lakes in China are faced with ecological and environmental problems. The five most famous lakes of Jiangsu Province, Taihu Lake, Gehu Lake, Gaobaoshaobo Lake, Hongze Lake, and Luoma Lake, have long-term fixed monitoring points for water body-related indicators. Over a five-year period, the monitoring showed that Gehu Lake had the highest average total nitrogen (TN) and total phosphorus (TP) concentrations among all lakes which were close to the Grade V critical value of the China’s Environmental Quality Standards for Surface Water (CEQSW). The NH3-N concentrations in all lakes were Grade IV according to the China’s Water Quality Standard for Drinking Water Sources (CWQSDWS) and Grade II according to the CEQSW. In addition, although TP concentrations in Taihu Lake did not exceed Grade V in the CEQSW, TP removal was the main factor controlling eutrophication. It was also found that the petroleum concentrations in all lakes were lower than the Grade I according to the CEQSW. Despite this relatively low petroleum pollution, the concentration of petroleum was negatively correlated with the phytoplankton densities in all lakes. This indicated that phytoplankton density was very sensitive to petroleum concentration. For heavy metals, the concentrations of Pb, Cu, As, and Cd in all lakes were significantly lower than Grade I (CEQSW) from 2013 to 2017. However, the accumulated heavy metals in sediments will remain an important pollution source affecting water quality and aquatic products in the future. The comprehensive pollution index analysis showed that the five lakes were often moderately polluted, indicating that the protection of lake resources in China should not be relaxed for a long time in the future.
Emission Characteristics of NOx and SO2 during the Combustion of Antibiotic Mycelial Residue
Authors: Ge Yaxin , Zhang Guangyi , Zhang Jianling , Zhang Wennan , Cui Lijie
Published: January 2022

The antibiotic mycelial residue (AMR) generated from cephalosporin C production is a hazardous organic waste, which is usually disposed of by landfilling that causes potential secondary environmental pollution. AMR combustion can be an effective method to treat AMR. In order to develop clean combustion technologies for safe disposal and energy recovery from various AMRs, the emission characteristics of NOx and SO2 from AMR combustion were studied experimentally in this work. It was found that the fuel-N is constituted by 85% protein nitrogen and 15% inorganic nitrogen, and the fuel-S by 78% inorganic sulfur and 22% organic sulfur. Nitrogen oxide emissions mainly occur at the volatile combustion stage when the temperature rises to 400 °C, while the primary sulfur oxide emission appears at the char combustion stage above 400 °C. Increasing the combustion temperature and airflow cause higher NOx emissions. High moisture content in AMR can significantly reduce the NOx emission by lowering the combustion temperature and generating more reducing gases such as CO. For the SO2 emission, the combustion temperature (700 to 900 °C), airflow and AMR water content do not seem to exhibit obvious effects. The presence of CaO significantly inhibits SO2 emission, especially for the SO2 produced during the AMR char combustion because of the good control effect on the direct emission of inorganic SO2. Employing air/fuel staging technologies in combination with in-situ desulfurization by calcium oxide/salts added in the combustor with operation temperatures lower than 900 °C should be a potential technology for the clean disposal of AMRs.
The Interaction Effects of Aeration and Plant on the Purification Performance of Horizontal Subsurface Flow Constructed Wetland
Authors: Chen Xinyi , Zhong Fei , Chen Yue , Wu Juan , Cheng Shuiping
Published: January 2022

Aeration and plants exhibit influence on the water purification performance in constructed wetlands (CWs). However, the interaction between aeration and plants on enhancing performance of domestic sewage treatment is unclear. Our study aims to optimize the combination of aeration position and plant species, promoting the extensive and effective application of CWs. Herein, six horizontal subsurface flow (HSSF) CWs small scale plots were established and divided into two groups according to the plant (i.e., Canna indica and Iris sibirica). To adjust the distribution of dissolved oxygen (DO) in CWs, each group had three plots of HSSF CWs. One plot was aerated at the bottom of the first quarter of the filtration chamber, one plot was aerated at the bottom of the inflow chamber, and the remaining plot was not aerated as a control. Results showed that aeration at the bottom of the first quarter filtration chamber could contribute to the highest removal efficiency of chemical oxygen demand (COD), ammonium nitrogen (NH4+-N) and total nitrogen (TN). The COD, NH4+-N, and TN removal percentages decreased with the drop in temperature. However, the plot aerated at the bottom of the first quarter filtration chamber with I. sibirica exhibited the best average CODCr, NH4+-N and TN removal percentages in both the warm season (83.6%, 82.7% and 76.8%) and the cool season (66.3%, 44.1% and 43.8%). Therefore, this study indicated that the combination of aerating at the bottom of the first quarter filtration chamber and planting with I. sibirica in the HSSF CWs would be a promising way forward for wastewater treatment, especially in low temperature seasons.
Rural–Urban Differences in Behavioral Outcomes among Adults with Lifetime History of Traumatic Brain Injury with Loss of Consciousness: 2016–2019 Ohio BRFSS
Authors: Feiss Robyn , Corrigan John D. , Ding Kele , Beaulieu Cynthia L. , Bogner Jennifer , Yang Jingzhen
Published: February 2022

This study examined if the associations between lifetime history of traumatic brain injury (TBI) with loss of consciousness (LOC) and unhealthy alcohol use or mental health problems differ by location of living (rural vs. urban). The lifetime history data of TBI with LOC, location of living, unhealthy alcohol use (binge drinking, heavy drinking), and mental health problems (depression diagnosis, number of poor mental health days) were sourced from the 2016, 2017, 2018, and 2019 Ohio Behavioral Risk Factory Surveillance Surveys, and the final sample included 16,941 respondents. We conducted multivariable logistic regressions to determine the odds ratios for each of the five outcomes between individuals living in rural vs. urban areas and between individuals with vs. without a lifetime history of TBI with LOC. No interaction between location of living and lifetime history of TBI with LOC was observed for any outcomes, indicating rurality did not modify these relationships. Living in a rural area was associated with decreased binge drinking or heavy drinking but not mental health outcomes. Lifetime history of TBI with LOC was associated with an increased risk of binge drinking, heavy drinking, depression diagnoses, and poor general mental health, regardless of location of living. Our findings support the need for TBI screenings as part of mental health intake evaluations and behavioral health screenings. Though rurality was not associated with mental health outcomes, rural areas may have limited access to quality mental health care. Therefore, future research should address access to mental health services following TBI among rural residents.
Occupational Health and Safety Statistics as an Indicator of Worker Physical Health in South African Industry
Authors: Rikhotso Oscar , Morodi Thabiso John , Masekameni Daniel Masilu
Published: February 2022

Operations in general industry, including manufacturing, expose employees to a myriad of occupational health hazards. To prevent exposure, occupational health and safety regulations were enacted, with both employers and workers instituting various risk reduction measures. The analysis of available occupational disease and injury statistics (indicators of worker physical health) can be used to infer the effectiveness of risk reduction measures and regulations in preventing exposure. Thus, using the READ approach, analyses of occupational disease and injury statistics from South African industry, derived from annual reports of the Compensation Fund, were conducted. The publicly available database of occupational disease and injury statistics from the South African general industry is unstructured, and the data are inconsistently reported. This data scarcity, symptomatic of an absence of a functional occupational disease surveillance system, complicates judgement making regarding the effectiveness of implemented risk reduction measures, enacted occupational health and safety regulations and the status of worker physical health from exposure to workplace hazards. The statistics, where available, indicate that workers continue to be exposed to occupational health impacts within general industry, notwithstanding risk reduction measures and enacted regulations. In particular, worker physical health continues to be impacted by occupational injuries and noise-induced hearing loss. This is suggestive of shortcomings and inefficiencies in industry-implemented preventive measures and the regulatory state. A robust national occupational disease surveillance system is a regulatory tool that should detect and direct policy responses to identified occupational health hazards.
Fiberglass and Other Flame-Resistant Fibers in Mattress Covers
Authors: Wagner Jeff , Fowles Jefferson , Barreau Tracy
Published: February 2022

Public complaints have raised concerns that some mattresses in the current marketplace may be potential sources of airborne fiberglass. Although mattress foam is often marketed as chemical-free, their cover compositions are not as well understood by the general public. To fill these basic information gaps, the covers of four newly purchased mattresses were sampled and analyzed using polarized light microscopy, SEM-EDS, and FTIR microspectroscopy. Two of the mattress covers contained over 50% fiberglass in their inner sock layers. Up to 1% of the fiberglass had migrated to adjacent fabric layers, representing a potential risk of consumer exposure if the zipper on the outer cover is opened. The observed fiberglass fragments had calculated aerodynamic diameters ranging between 30 and 50 µm, suggesting they are potentially inhalable into the nose, mouth, and throat, but are likely too large to penetrate deeper into the lungs. No fiberglass was observed on the brand new mattresses’ outer surfaces. Synthetic fibers also present in the sock layers were consistent with flame resistant modacrylic containing vinyl chloride and antimony. The use of fiberglass and other chemicals in mattress covers poses a potential health risk if these materials are not adequately contained. The apparent non-inclusion of mattress covers in chemical-free certifications suggests that further improvements are needed in mattress labeling and education of consumers.
GisGCN: A Visual Graph-Based Framework to Match Geographical Areas through Time
Authors: Khokhlova Margarita , Abadie Nathalie , Gouet-Brunet Valérie , Chen Liming
Published: January 2022

Historical visual sources are particularly useful for reconstructing the successive states of the territory in the past and for analysing its evolution. However, finding visual sources covering a given area within a large mass of archives can be very difficult if they are poorly documented. In the case of aerial photographs, most of the time, this task is carried out by solely relying on the visual content of the images. Convolutional Neural Networks are capable to capture the visual cues of the images and match them to each other given a sufficient amount of training data. However, over time and across seasons, the natural and man-made landscapes may evolve, making historical image-based retrieval a challenging task. We want to approach this cross-time aerial indexing and retrieval problem from a different novel point of view: by using geometrical and topological properties of geographic entities of the researched zone encoded as graph representations which are more robust to appearance changes than the pure image-based ones. Geographic entities in the vertical aerial images are thought of as nodes in a graph, linked to each other by edges representing their spatial relationships. To build such graphs, we propose to use instances from topographic vector databases and state-of-the-art spatial analysis methods. We demonstrate how these geospatial graphs can be successfully matched across time by means of the learned graph embedding.
The “Elastic Perspective” of SARS-CoV-2 Infection and the Role of Intrinsic and Extrinsic Factors
Authors: Boraldi Federica , Lofaro Francesco Demetrio , Cossarizza Andrea , Quaglino Daniela
Published: January 2022

Elastin represents the structural component of the extracellular matrix providing elastic recoil to tissues such as skin, blood vessels and lungs. Elastogenic cells secrete soluble tropoelastin monomers into the extracellular space where these monomers associate with other matrix proteins (e.g., microfibrils and glycoproteins) and are crosslinked by lysyl oxidase to form insoluble fibres. Once elastic fibres are formed, they are very stable, highly resistant to degradation and have an almost negligible turnover. However, there are circumstances, mainly related to inflammatory conditions, where increased proteolytic degradation of elastic fibres may lead to consequences of major clinical relevance. In severely affected COVID-19 patients, for instance, the massive recruitment and activation of neutrophils is responsible for the profuse release of elastases and other proteolytic enzymes which cause the irreversible degradation of elastic fibres. Within the lungs, destruction of the elastic network may lead to the permanent impairment of pulmonary function, thus suggesting that elastases can be a promising target to preserve the elastic component in COVID-19 patients. Moreover, intrinsic and extrinsic factors additionally contributing to damaging the elastic component and to increasing the spread and severity of SARS-CoV-2 infection are reviewed.
YdfD, a Lysis Protein of the Qin Prophage, Is a Specific Inhibitor of the IspG-Catalyzed Step in the MEP Pathway of Escherichia coli
Authors: Lu Zhifang , Wang Biying , Qiu Zhiyu , Zhang Ruiling , Zheng Jimin , Jia Zongchao
Published: January 2022

Bacterial cryptic prophage (defective prophage) genes are known to drastically influence host physiology, such as causing cell growth arrest or lysis, upon expression. Many phages encode lytic proteins to destroy the cell envelope. As natural antibiotics, only a few lysis target proteins were identified. ydfD is a lytic gene from the Qin cryptic prophage that encodes a 63-amino-acid protein, the ectopic expression of which in Escherichia coli can cause nearly complete cell lysis rapidly. The bacterial 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is responsible for synthesizing the isoprenoids uniquely required for sustaining bacterial growth. In this study, we provide evidence that YdfD can interact with IspG, a key enzyme involved in the MEP pathway, both in vivo and in vitro. We show that intact YdfD is required for the interaction with IspG to perform its lysis function and that the mRNA levels of ydfD increase significantly under certain stress conditions. Crucially, the cell lysis induced by YdfD can be abolished by the overexpression of ispG or the complementation of the IspG enzyme catalysis product methylerythritol 2,4-cyclodiphosphate. We propose that YdfD from the Qin cryptic prophage inhibits IspG to block the MEP pathway, leading to a compromised cell membrane and cell wall biosynthesis and eventual cell lysis.
Short-Term Immune Responses of Gilthead Seabream (Sparus aurata) Juveniles against Photobacterium damselae subsp. piscicida
Authors: Santos Paulo , Peixoto Diogo , Ferreira Inês , Passos Ricardo , Pires Pedro , Simões Marco , Pousão-Ferreira Pedro , Baptista Teresa , Costas Benjamín
Published: January 2022

Photobacteriosis is a septicaemic bacterial disease affecting several marine species around the globe, resulting in significant economic losses. Although many studies have been performed related to the pathogen virulence and resistance factors, information regarding the host defence mechanisms activated once an infection takes place is still scarce. The present study was designed to understand innate immune responses of farmed juvenile gilthead seabream (Sparus aurata) after Photobacterium damselae subsp. piscicida (Phdp) infection. Therefore, two groups of seabream juveniles were intraperitoneally injected with 100 µL of PBS (placebo) or 100 µL of exponentially growing Phdp (1 × 106 CFU/mL; infected). The blood, plasma, liver, and head kidney of six fish from each treatment were sampled immediately before infection and 3, 6, 9, 24 and 48 h after infection for the broad screening of fish immune and oxidative stress responses. Infected animals presented marked anaemia, neutrophilia and monocytosis, conditions that are correlated with an increased expression of genes related to inflammation and phagocytic activity. Similar studies with different fish species and bacteria can be useful for the definition of health biomarkers that might help fish farmers to prevent the occurrence of such diseases.
Proliferation and Apoptosis Pathways and Factors in Oral Squamous Cell Carcinoma
Authors: He Steven , Chakraborty Rajdeep , Ranganathan Shoba
Published: January 2022

Oral cancer is the most common form of head and neck squamous cell carcinoma (HNSCC) and most frequently presents as oral squamous cell carcinoma (OSCC), which is associated with an alarmingly high mortality rate. Internationally, a plethora of research to further our understanding of the molecular pathways related to oral cancer is performed. This research is of value for early diagnosis, prognosis, and the investigation of new drugs that can ameliorate the harmful effects of oral cancer and provide optimal patient outcomes with minimal long-term complications. Two pathways on which the progression of OSCC depends on are those of proliferation and apoptosis, which overlap at many junctions. Herein, we aim to review these pathways and factors related to OSCC progression. Publicly available search engines, PubMed and Google Scholar, were used with the following keywords to identify relevant literature: oral cancer, proliferation, proliferation factors, genes, mutations, and tumor suppressor. We anticipate that the use of information provided through this review will further progress translational cancer research work in the field of oral cancer.
Overview of First-Line and Second-Line Pharmacotherapies for Osteoarthritis with Special Focus on Intra-Articular Treatment
Authors: Nowaczyk Alicja , Szwedowski Dawid , Dallo Ignacio , Nowaczyk Jacek
Published: January 2022

Osteoarthritis (OA) can be defined as the result of pathological processes of various etiologies leading to damage to the articular structures. Although the mechanism of degenerative changes has become better understood due to the plethora of biochemical and genetic studies, the drug that could stop the degenerative cascade is still unknown. All available forms of OA therapy are based on symptomatic treatment. According to actual guidelines, comprehensive treatment of OA should always include a combination of various therapeutic options aimed at common goals, which are pain relief in the first place, and then the improvement of function. Local treatment has become more common practice, which takes place between rehabilitation and pharmacological treatment in the hierarchy of procedures. Only in the case of no improvement and the presence of advanced lesions visible in imaging tests, should surgery be considered. Currently, an increasing number of studies are being published suggesting that intra-articular injections may be as effective or even more effective than non-steroidal anti-inflammatory drugs (NSAIDs) and result in fewer systemic adverse events. The most commonly used preparations are hyaluronic acid (HA), glucocorticosteroids (GS), and also platelet-rich plasma (PRP) in recent years. This review aims to present the mechanism of action and clinical effectiveness of different pharmacological options in relieving pain and improving functions in OA as well as the emerging approach in intra-articular treatment with PRP.
Usher Syndrome Belongs to the Genetic Diseases Associated with Radiosensitivity: Influence of the ATM Protein Kinase
Authors: Al-Choboq Joëlle , Ferlazzo Mélanie L. , Sonzogni Laurène , Granzotto Adeline , El-Nachef Laura , Maalouf Mira , Berthel Elise , Foray Nicolas
Published: January 2022

Usher syndrome (USH) is a rare autosomal recessive disease characterized by the combination of hearing loss, visual impairment due to retinitis pigmentosa, and in some cases vestibular dysfunctions. Studies published in the 1980s reported that USH is associated with cellular radiosensitivity. However, the molecular basis of this particular phenotype has not yet been documented. The aim of this study was therefore to document the radiosensitivity of USH1—a subset of USH—by examining the radiation-induced nucleo-shuttling of ATM (RIANS), as well as the functionality of the repair and signaling pathways of the DNA double-strand breaks (DSBs) in three skin fibroblasts derived from USH1 patients. The clonogenic cell survival, the micronuclei, the nuclear foci formed by the phosphorylated forms of the X variant of the H2A histone (ɣH2AX), the phosphorylated forms of the ATM protein (pATM), and the meiotic recombination 11 nuclease (MRE11) were used as cellular and molecular endpoints. The interaction between the ATM and USH1 proteins was also examined by proximity ligation assay. The results showed that USH1 fibroblasts were associated with moderate but significant radiosensitivity, high yield of micronuclei, and impaired DSB recognition but normal DSB repair, likely caused by a delayed RIANS, suggesting a possible sequestration of ATM by some USH1 proteins overexpressed in the cytoplasm. To our knowledge, this report is the first radiobiological characterization of cells from USH1 patients at both molecular and cellular scales.
Non-TZF Transcriptional Activator AtC3H12 Negatively Affects Seed Germination and Seedling Development in Arabidopsis
Authors: Seok Hye-Yeon , Kim Taehyoung , Lee Sun-Young , Moon Yong-Hwan
Published: January 2022

CCCH zinc finger proteins are a large protein family and are classified as either tandem CCCH zinc finger (TZF) or non-TZF proteins. The roles of TZF genes in several plants have been well determined, whereas the functions of many non-TZF genes in plants remain uncharacterized. Herein, we describe biological and molecular functions of AtC3H12, an Arabidopsis non-TZF protein containing three CCCH zinc finger motifs. AtC3H12 has orthologs in several plant species but has no paralog in Arabidopsis. AtC3H12-overexpressing transgenic plants (OXs) germinated slower than wild-type (WT) plants, whereas atc3h12 mutants germinated faster than WT plants. The fresh weight (FW) and primary root lengths of AtC3H12 OX seedlings were lighter and shorter than those of WT seedlings, respectively. In contrast, FW and primary root lengths of atc3h12 seedlings were heavier and longer than those of WT seedlings, respectively. AtC3H12 was localized in the nucleus and displayed transactivation activity in both yeast and Arabidopsis. We found that the 97–197 aa region of AtC3H12 is an important part for its transactivation activity. Detection of expression levels and analysis of Arabidopsis transgenic plants harboring a PAtC3H12::GUS construct showed that AtC3H12 expression increases as the Arabidopsis seedlings develop. Taken together, our results demonstrate that AtC3H12 negatively affects seed germination and seedling development as a nuclear transcriptional activator in Arabidopsis. To our knowledge, this is the first report to show that non-TZF proteins negatively affect plant development as nuclear transcriptional activators.
Multi Platforms Strategies and Metabolomics Approaches for the Investigation of Comprehensive Metabolite Profile in Dogs with Babesia canis Infection
Authors: Rubić Ivana , Burchmore Richard , Weidt Stefan , Regnault Clement , Kuleš Josipa , Barić Rafaj Renata , Mašek Tomislav , Horvatić Anita , Crnogaj Martina , Eckersall Peter David , Novak Predrag , Mrljak Vladimir
Published: January 2022

Canine babesiosis is an important tick-borne disease worldwide, caused by parasites of the Babesia genus. Although the disease process primarily affects erythrocytes, it may also have multisystemic consequences. The goal of this study was to explore and characterize the serum metabolome, by identifying potential metabolites and metabolic pathways in dogs naturally infected with Babesia canis using liquid and gas chromatography coupled to mass spectrometry. The study included 12 dogs naturally infected with B. canis and 12 healthy dogs. By combining three different analytical platforms using untargeted and targeted approaches, 295 metabolites were detected. The untargeted ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) metabolomics approach identified 64 metabolites, the targeted UHPLC-MS/MS metabolomics approach identified 205 metabolites, and the GC-MS metabolomics approach identified 26 metabolites. Biological functions of differentially abundant metabolites indicate the involvement of various pathways in canine babesiosis including the following: glutathione metabolism; alanine, aspartate, and glutamate metabolism; glyoxylate and dicarboxylate metabolism; cysteine and methionine metabolism; and phenylalanine, tyrosine, and tryptophan biosynthesis. This study confirmed that host–pathogen interactions could be studied by metabolomics to assess chemical changes in the host, such that the differences in serum metabolome between dogs with B. canis infection and healthy dogs can be detected with liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) methods. Our study provides novel insight into pathophysiological mechanisms of B. canis infection.
Muscle Research: A Tour d’Horizon
Authors: Zancanaro Carlo
Published: January 2022

The Special Issue on the “Muscular Structure, Physiology and Metabolism” was proposed in order to maintain the referenced scientific community abreast with recent research advancements regarding the morphology, functionality, and metabolism of muscle tissue, including a total of eighteen published papers, of which twelve were original research manuscripts and six were review papers [...]
Anti-Thrombotic Effects of Artesunate through Regulation of cAMP and PI3K/MAPK Pathway on Human Platelets
Authors: Yoon Shin-Sook , Kwon Hyuk-Woo , Shin Jung-Hae , Rhee Man Hee , Park Chang-Eun , Lee Dong-Ha
Published: January 2022

Normal activation of platelets and their aggregation are crucial for proper hemostasis. It appears that excessive or abnormal aggregation of platelets may bring about cardiovascular diseases such as stroke, atherosclerosis, and thrombosis. For this reason, finding a substance that can regulate platelet aggregation or suppress aggregation will aid in the prevention and treatment of cardiovascular diseases. Artesunate is a compound extracted from the plant roots of Artemisia or Scopolia, and its effects have shown to be promising in areas of anticancer and Alzheimer’s disease. However, the role and mechanisms by which artesunate affects the aggregation of platelets and the formation of a thrombus are currently not understood. This study examines the ways artesunate affects the aggregation of platelets and the formation of a thrombus on platelets induced by U46619. As a result, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) production were increased significantly by artesunate relative to the doses, as well as phosphorylated vasodilator-stimulated phosphoprotein (VASP) and inositol 1,4,5-trisphosphate receptor (IP3R), substrates to cAMP-dependent kinase and cGMP-dependent kinase, in a significant manner. The Ca2+, normally mobilized from the dense tubular system, was inhibited due to IP3R phosphorylation from artesunate, and phosphorylated VASP aided in inhibiting platelet activity via αIIb/β3 platelet membrane inactivation and inhibiting fibrinogen binding. In addition, MAPK and PI3K/Akt phosphorylation was inhibited via artesunate in a significant manner, causing the production of TXA2 and intracellular granular secretion (serotonin and ATP release) to be reduced. Therefore, we suggest that artesunate has value as a substance that inhibits platelet aggregation and thrombus formation through an antiplatelet mechanism.
MHY2245, a Sirtuin Inhibitor, Induces Cell Cycle Arrest and Apoptosis in HCT116 Human Colorectal Cancer Cells
Authors: Kang Yong Jung , Jang Jung Yoon , Kwon Young Hoon , Lee Jun Ho , Lee Sanggwon , Park Yujin , Jung Young-Suk , Im Eunok , Moon Hyung Ryong , Chung Hae Young , Kim Nam Deuk
Published: January 2022

Sirtuins (SIRTs), which are nicotinamide adenine dinucleotide-dependent class III histone deacetylases, regulate cell division, survival, and senescence. Although sirtinol, a synthetic SIRT inhibitor, is known to exhibit antitumor effects, its mechanism of action is not well understood. Therefore, we aimed to assess the anticancer effects and underlying mechanism of MHY2245, a derivative of sirtinol, in HCT116 human colorectal cancer cells in vitro. Treatment with MHY2245 decreased SIRT1 activity and caused DNA damage, leading to the upregulation of p53 acetylation, and increased levels of p53, phosphorylation of H2A histone family member X, ataxia telangiectasia and Rad3-related kinase, checkpoint kinase 1 (Chk1), and Chk2. The level of the breast cancer type 1 susceptibility protein was also found to decrease. MHY2245 induced G2/M phase cell cycle arrest via the downregulation of cyclin B1, cell division cycle protein 2 (Cdc2), and Cdc25c. Further, MHY2245 induced HCT116 cell death via apoptosis, which was accompanied by internucleosomal DNA fragmentation, decreased B-cell lymphoma 2 (Bcl-2) levels, increased Bcl-2-asscociated X protein levels, cleavage of poly(ADP-ribose) polymerase, and activation of caspases -3, -8, and -9. Overall, MHY2245 induces cell cycle arrest, triggers apoptosis through caspase activation, and exhibits DNA damage response-associated anticancer effects.
Structural Insights into the Substrate Transport Mechanisms in GTR Transporters through Ensemble Docking
Authors: Peña-Varas Carlos , Kanstrup Christa , Vergara-Jaque Ariela , González-Avendaño Mariela , Crocoll Christoph , Mirza Osman , Dreyer Ingo , Nour-Eldin Hussam , Ramírez David
Published: January 2022

Glucosinolate transporters (GTRs) are part of the nitrate/peptide transporter (NPF) family, members of which also transport specialized secondary metabolites as substrates. Glucosinolates are defense compounds derived from amino acids. We selected 4-methylthiobutyl (4MTB) and indol-3-ylmethyl (I3M) glucosinolates to study how GTR1 from Arabidopsis thaliana transports these substrates in computational simulation approaches. The designed pipeline reported here includes massive docking of 4MTB and I3M in an ensemble of GTR1 conformations (in both inward and outward conformations) extracted from molecular dynamics simulations, followed by clustered and substrate–protein interactions profiling. The identified key residues were mutated, and their role in substrate transport was tested. We were able to identify key residues that integrate a major binding site of these substrates, which is critical for transport activity. In silico approaches employed here represent a breakthrough in the plant transportomics field, as the identification of key residues usually takes a long time if performed from a purely wet-lab experimental perspective. The inclusion of structural bioinformatics in the analyses of plant transporters significantly speeds up the knowledge-gaining process and optimizes valuable time and resources.
Catalytic Sulfation of Betulin with Sulfamic Acid: Experiment and DFT Calculation
Authors: Kazachenko Aleksandr S. , Akman Feride , Vasilieva Natalya Yu. , Issaoui Noureddine , Malyar Yuriy N. , Kondrasenko Aleksandr A. , Borovkova Valentina S. , Miroshnikova Angelina V. , Kazachenko Anna S. , Al-Dossary Omar , Wojcik Marek J. , Berezhnaya Yaroslava D. , Elsuf’ev Evgeniy V.
Published: January 2022

Betulin is an important triterpenoid substance isolated from birch bark, which, together with its sulfates, exhibits important bioactive properties. We report on a newly developed method of betulin sulfation with sulfamic acid in pyridine in the presence of an Amberlyst®15 solid acid catalyst. It has been shown that this catalyst remains stable when being repeatedly (up to four cycles) used and ensures obtaining of sulfated betulin with a sulfur content of ~10%. The introduction of the sulfate group into the betulin molecule has been proven by Fourier-transform infrared, ultraviolet-visible, and nuclear magnetic resonance spectroscopy. The Fourier-transform infrared (FTIR) spectra contain absorption bands at 1249 and 835–841 cm−1; in the UV spectra, the peak intensity decreases; and, in the nuclear magnetic resonance (NMR) spectra, of betulin disulfate, carbons С3 and С28 are completely shifted to the weak-field region (to 88.21 and 67.32 ppm, respectively) with respect to betulin. Using the potentiometric titration method, the product of acidity constants K1 and K2 of a solution of the betulin disulfate H+ form has been found to be 3.86 × 10–6 ± 0.004. It has been demonstrated by the thermal analysis that betulin and the betulin disulfate sodium salt are stable at temperatures of up to 240 and 220 °C, respectively. The density functional theory method has been used to obtain data on the most stable conformations, molecular electrostatic potential, frontier molecular orbitals, and mulliken atomic charges of betulin and betulin disulfate and to calculate the spectral characteristics of initial and sulfated betulin, which agree well with the experimental data.
A Comprehensive Evaluation of Salt Tolerance in Tomato (Var. Ailsa Craig): Responses of Physiological and Transcriptional Changes in RBOH’s and ABA Biosynthesis and Signalling Genes
Authors: Raziq Abdul , Wang Yu , Mohi Ud Din Atta , Sun Jin , Shu Sheng , Guo Shirong
Published: January 2022

Salinity is a ubiquitous stressor, depleting osmotic potential and affecting the tomato seedlings’ development and productivity. Considering this critical concern, we explored the salinity response in tomato seedlings by evaluating them under progressive salt stress duration (0, 3, 6, and 12 days). Intriguingly, besides the adverse effect of salt stress on tomato growth the findings exhibited a significant role of tomato antioxidative system, RBOH genes, ABA biosynthesis, and signaling transcription factor for establishing tolerance to salinity stress. For instance, the activities of enzymatic and non-enzymatic antioxidants continued to incline positively with the increased levels of reactive oxygen species (O2•−, H2O2), MDA, and cellular damage, suggesting the scavenging capacity of tomato seedlings against salt stress. Notably, the RBOH transcription factors activated the hydrogen peroxide-mediated signalling pathway that induced the detoxification mechanisms in tomato seedlings. Consequently, the increased gene expression of antioxidant enzymes and the corresponding ratio of non-enzymatic antioxidants AsA-GSH suggested the modulation of antioxidants to survive the salt-induced oxidative stress. In addition, the endogenous ABA level was enhanced under salinity stress, indicating higher ABA biosynthesis and signalling gene expression. Subsequently, the upregulated transcript abundance of ABA biosynthesis and signalling-related genes suggested the ABA-mediated capacity of tomato seedlings to regulate homeostasis under salt stress. The current findings have revealed fascinating responses of the tomato to survive the salt stress periods, in order to improve the abiotic stress tolerance in tomato.
A Bidirectional Context Embedding Transformer for Automatic Speech Recognition
Authors: Liao Lyuchao , Afedzie Kwofie Francis , Chen Zhifeng , Han Guangjie , Wang Yongqiang , Lin Yuyuan , Hu Dongmei
Published: January 2022

Transformers have become popular in building end-to-end automatic speech recognition (ASR) systems. However, transformer ASR systems are usually trained to give output sequences in the left-to-right order, disregarding the right-to-left context. Currently, the existing transformer-based ASR systems that employ two decoders for bidirectional decoding are complex in terms of computation and optimization. The existing ASR transformer with a single decoder for bidirectional decoding requires extra methods (such as a self-mask) to resolve the problem of information leakage in the attention mechanism This paper explores different options for the development of a speech transformer that utilizes a single decoder equipped with bidirectional context embedding (BCE) for bidirectional decoding. The decoding direction, which is set up at the input level, enables the model to attend to different directional contexts without extra decoders and also alleviates any information leakage. The effectiveness of this method was verified with a bidirectional beam search method that generates bidirectional output sequences and determines the best hypothesis according to the output score. We achieved a word error rate (WER) of 7.65%/18.97% on the clean/other LibriSpeech test set, outperforming the left-to-right decoding style in our work by 3.17%/3.47%. The results are also close to, or better than, other state-of-the-art end-to-end models.