bluuebunny/crossref_metadata_2025 huggingface dataset:
Construction of CRISPR/Cas9 vector for editing IaVQ9 gene in Ipomoea aquatica
Authors: Hue Huynh Thi Thu , Ngoc Nguyen Thi Bich , Thanh Le Tat , Hoa Nguyen Thi , Tri Le Minh , Thuy Le Thi Bich
Published: November 2024

The group of proteins containing the VQ motif (named VQ proteins) is a family of plant-specific proteins with a FxxhVQxhTG conservative VQ-motif region. VQ proteins regulate many developmental processes, including responses to biotic and abiotic stresses, and seed development. The VQ9 protein has an interaction with the WRKY8 factor, when this interaction occurs, it causes a decrease in the DNA binding ability of WRKY8 to DNA, which plays a role in the regulation function of the plant to stress. Some mutations in the VQ9 gene increase salt tolerance in plants, suggesting that VQ9 acts antagonistically to regulate responses to salt conditions. This antagonism is consistent with an increase or decrease in the Na+/K+ ratio. Ipomoea aquatica is commonly grown and used as a vegetable in Southeast Asia. The research involved RNA extraction from I. aquatica leaves, followed by PCR sequencing to confirm the presence of the IaVQ9 gene. Subsequently, a specific guide RNA (gRNA) was designed using CRISPR-P ver.2.0 and inserted into the pRGEB31 vector, optimized for CRISPR/Cas9 applications. The gRNA-inserted vector was successfully transformed into E. coli DH10B and then into Agrobacterium tumefaciens EHA105, verified through colony PCR and restriction enzyme analysis. This process created a delivery system capable of editing the VQ9 gene in I. aquatica. This research represents a significant step towards improving crop resilience to salinity, addressing a critical challenge for agriculture in salt-affected regions. Future studies will focus on transferring the construct back into I. aquatica plants to assess its impact on enhancing salt tolerance, potentially contributing to sustainable crop production in adverse environmental conditions.
A colorimetric multiplex RPA approach for on-site dual monitoring of Vibrio parahaemolyticus and White Spot Syndrome Virus in Pacific Whiteleg shrimp
Authors: Tran Diem Hong , Giang Vo Ngoc Truc , Phung Huong Thi Thu , Bui Le Minh
Published: November 2024

White spot syndrome virus (WSSV) and Vibrio parahaemolyticus represent the most prevalent and serious pathogens in Viet Nam's shrimp industry, annually inflicting major production losses. With no existing therapeutic measures, timely and accurate diagnosis is imperative for curbing outbreaks and limiting economic impacts through isolation and culling protocols. However, conventional techniques like polymerase chain reaction (PCR) and quantitative PCR require advanced laboratory infrastructure that is incompatible with rapid on-site pathogen surveillance. This work details the development of a multiplex recombinase polymerase amplification (RPA) assay for the simultaneous detection of WSSV and V. parahaemolyticus using a visual colorimetric readout amenable to field deployment. Primers targeting unique WSSV and V. parahaemolyticus sequences were designed for selective amplification. Specificity screening verified exclusive pathogen detection against common shrimp microbiota and human-handling contaminants with no cross-reactivity. Singleplex reactions identified 39 °C for 35 minutes as optimal conditions; hence, these parameters were subsequently applied in multiplex format. Incorporation of the Mg2+-sensitive eriochrome black T (EBT) dye enabled clear discrimination between positive blue and negative violet reactions by the naked eye. The multiplex assay demonstrated high analytical sensitivity, down to 1 copy of DNA template per reaction. Clinical validation of the multiplex RPA method using shrimp samples versus quantitative PCR showed 100% agreement. By coupling rapid isothermal amplification with visual indicator-based detection in a multiplexed format, this assay provides simple, robust, and user-friendly identification of major shrimp pathogens in resource-limited settings in less than one hour to facilitate prompt on-site disease control decisions. The field-suitable platform aims to mitigate outbreak magnitude and economic consequences through timely response.
Antimicrobial activity of chitosan and combination with antibiotics against mastitis-causing pathogens
Authors: Huynh Thi Kieu Oanh , Pham Thi Minh Khanh , Huynh Thuc Quyen , Tran Van Ty , Nguyen Quynh Thuong , Ngo Hong Phuong , Nguyen Phuong Thao , Nguyen Thi Thu Hoai
Published: November 2024

Bovine mastitis (BM), primarily caused by bacterial pathogens infecting mammary glands, stands as the most prevalent disease in dairy cattle. Traditionally, antibiotics have been the primary choice of treatment, yet their overuse has led to widespread resistance and the presence of antibiotic residues in dairy products. Today, chitosan has emerged as a promising alternative in dairy farming. In this study, we systematically screened and assessed the antibacterial efficacy of five chitosan preparations of different viscosities and components. Additionally, we explored the synergistic antimicrobial potential of the most potent chitosan sample in combination with commonly employed antibiotics, including ampicillin, amoxicillin, oxacillin, and levofloxacin against four prevalent BM-causing pathogens: Staphylococcus epidermidis, Streptococcus agalactiae, Streptococcus uberis and Pseudomonas sp. Agar well diffusion, micro-dilution, and checkerboard techniques were applied to assess the antimicrobial activity and interaction effect. Results indicated that, at a concentration of 1%, low and medium viscosity samples (samples 1, 2, 3) exhibited relatively low activity, compared to very low viscosity ones (samples 4, 5). Notably, sample 5, a combination of chitosan sample 1 with orange and grapefruit essential oils, demonstrated the most potent antibacterial activity with a minimal inhibitory concentration (MIC) of 19.53 mg/L against S. agalactiae, S. uberis and S. epidermidis and 78.13 mg/L against Pseudomonas sp.. Furthermore, the combination of this chitosan sample and antibiotics exhibited some synergistic interactions against BM-causing pathogens, as indicated by the fractional inhibitory concentration (FIC) values ranging from ≥ 0.5 to ≤ 1. While these effects were notable, they did not reach the threshold for strong synergism (FIC < 0.5). In summary, our study highlighted the high antibacterial activity of low viscosity chitosan, particularly in combination with essential oils. Although there were observed synergistic effects with antibiotics against BM-causing pathogens, the strength of these interactions was not robust enough to conclusively categorize them as strongly synergistic. Chitosan, however, emerges as a promising agent in the ongoing exploration of alternatives to antibiotics in the management of BM in dairy farming.
Codon optimization, expression, and purification of the reverse transcriptase enzyme variant MMLV RT2 in Escherichia coli
Authors: Sang Nguyen Van , My Le Tra , Uyen Nguyen Thi , Anh Dang Thi Lan
Published: November 2024

Moloney leukemia virus reverse transcriptase (MMLV RT) is an important enzyme widely utilized in molecular biology research and various diagnostic testing kits. A previous study demonstrated that variants of MMLV RT containing specific mutations contribute to enhanced stability and increased capability for RNA detection. However, the optimization and increased expression level of MMLV RT variants for large-scale production have not been extensively studied. In this research, the gene segment encoding the MMLV RT variant (E286R/E302K/L435R/D524A) was codon optimized and subcloned into pET-M expression vector. The obtained pET-M MMLV RT2 was transformed into E. coli BL21(DE3) host cell system for over-expression of the MMLV RT2 enzyme. The recombinant enzyme was then purified using nickel affinity chromatography and quantified using the Bradford method. The results revealed significant increase in recombinant enzyme yield, achieving 0.25 g/L in the culture medium. This represents a 1.4-fold increase compared to the 0.175 g/L yield reported in the previous study. Enzyme MMLV RT2 was also evaluated for its activity and was found to have comparable activity to the commercial enzyme Promega and another variant, enzyme MMLV RT1, which carries the D524A mutation and has previously been evaluated for activity. This result suggests that a high expression level of a stable MMLV-RT enzyme variant can be achieved which is beneficial for further improving the production of this important enzyme. The high yield and sustained activity of the MMLV RT2 variant indicate its potential as a reliable and cost-effective alternative for various applications in research and diagnostics. The successful optimization and expression in E. coli highlight the feasibility of large-scale production, potentially lowering costs and improving accessibility for molecular biology applications.
Polymorphism of growth hormone 1 gene in co and Bach Thao breed goats: Is the recessive homozygous BB genotype lethal?
Authors: Dang Minh Lam , Le Quang , Nguyen Van Hop , Bui Anh Phu Nam
Published: November 2024

Goat is one of the most popular species in Vietnamese animal husbandry. However, phenotypic selection in goats is still popular in breeding programs. Thanks to the introduction of marker-assisted selection, genetics is currently playing a vital role in choosing the elite animals for breeding. One of the molecular markers that has been widely applied in animal husbandry is the GH1 gene (growth hormone 1), which encodes for the GH protein. GH has been known for its essential role in the growth and development of animals. In goats, GH1 polymorphism is significantly associated with performance traits. In this study, we aim to investigate the GH1 frequency in two indigenous goat breeds in Vietnam: the Co and Bach Thao breeds. Our results showed that there were only two genotypes AA and AB detected in Co and Bach Thao breeds. In the Co goat breed, the frequency of AA and AB genotypes was 0.1 and 0.9, respectively. The frequency of A and B alleles was 0.55 and 0.45, respectively. In the Bach Thao goat breed, the frequency of AA and AB genotypes was 0.16 and 0.84, respectively. The frequency of the A and B alleles was 0.58 and 0.42, respectively. Chi-square values obtained from the Co and Bach Thao goat breeds revealed that there was a significant deviation from the Hardy-Weinberg Equilibrium in both breeds (33.47 and 32.31, respectively, with P<0.001). The lack of BB genotype led us to hypothesize that there was overdominance in three genotypes of GH1 and that the BB homozygous genotype is lethal. We suggested that phenotypic observation would be recommended to measure the superiority of the AB genotype goat compared to the AA genotype goats. The results from this study would be beneficial to goat breeders in improving the goat breeding program in Vietnam.
Biocontrol potentiality of Burkholderia vietnamiensis nrv12 against the rice blast fungus magnaporthe oryzae
Authors: Loi Nguyen Thi Thanh , Anh Nguyen Tran Mai , Tuong Ho Manh , Nguyen Vu Thi Hanh , Tung Quach Ngoc , An Nguyen Thi Thu , Phat Do Tien , Ha Chu Hoang , Tien Phi Quyet
Published: November 2024

Rice blast disease, caused by the pathogenic fungus Magnaporthe oryzae, is a widespread infection leading to serious crop loss worldwide. In order to achieve sustainable agriculture, root-associated bacteria have been applied to manage fungal diseases and promote growth. The present study aimed to evaluate in vitro the growth-promoting ability and in vivo biocontrol activity against M. oryzae of rice rhizosphere bacterium. Out of sixty-eight isolates recovered from the rhizosphere of blast-infected rice plants, isolate NRV12 exhibited the highest antifungal activity against M. oryzae SH, with an inhibition percentage of 72.7±3.44%. By analysis of 16S rRNA sequence associated with morphology, physiological and biochemical tests, the strain was identified as Burkholderia vietnamiensis. In addition, NRV12 produced hydrolytic enzymes (amylase, cellulase, protease), indole acetic acid (IAA), exhibited nitrogen-fixing potential and the ability to solubilize phosphate and zinc. Innoculation with NRV12 significantly promoted in vivo rice seedling growth to 23.3% as compared to the non-bacteria-treated seedlings. Importantly, infected rice seedlings treated with NRV12 led to a 40% disease reduction in rice blast. These findings suggest that NRV12 is a valuable and promising isolate with biocontrol potential against rice blast caused by M. oryzae.
Research on isolation, identification, and determination of suitable conditions for the cultivation of albino Cordyceps militaris SHBTD fruiting bodies
Authors: Anh Dao Ngoc , Chien Truong Thi , Trang Do Thi Kim , Tram Tran Bao , Mai Nguyen Thi Thanh , Dung Thai Hanh , Tuan Tran Van , Tao Vu Xuan
Published: November 2024

Cordyceps militaris is a valuable fungus that is well-known in many Asian countries. The fruiting bodies of C. militaris are commonly used in traditional medicine and the production of functional foods. Currently, C. militaris mushrooms are being cultivated on a large scale in Vietnam, yielding the characteristic yellow-orange fruiting bodies typical of C. militaris. In the mushroom cultivation industry, C. militaris is one of the most highly valued species. The yield and quality of the fruiting bodies are greatly dependant on the strain source. Therefore, the search for new strains of C. militaris with high productivity, quality, and uniqueness is always of interest to scientists. This study has isolated and identified the albino strain C. militaris SHBTD based on morphological characteristics and the rDNA ITS region gene sequence. The optimal conditions for fruiting body formation of the SHBTD strain are MT6 medium, a temperature of 22°C, a humidity of 90%, a light intensity of 500 lux (12 hours/day), and 65 days of cultivation. Additionally, this study determined that the albino strain C. militaris SHBDT carrying the single Mating-type (MAT) gene (MAT1-1-1), could provide stable yield and quality of fruiting bodies over 5 consecutive cultivation generations. The obtained results demonstrate the potential applications of the C. militaris SHBTD in the industrial-scale production of unique and high-value mushroom products.
Discovery of Brevibacterium predominating in fecal samples from three children with persistent diarrhea negative for common pathogens
Authors: Quy Nguyen Thi , Khoa Dao Trong , Nga Pham Thi Thanh , Thuy Phung Thi Bich , Hang Nguyen Minh , Ha Nguyen Thi Viet , Huyen Do Thi
Published: November 2024

Acute diarrhea is a common disease in children under 5 years old and can develop into persistent diarrhea, greatly affecting the children's health. Although advanced techniques had been used to diagnose and detect common pathogens in hospitals, however, 40% of cases are negative for the pathogens. In this study, to investigate dominant bacteria in stool samples of three persistent-diarrheal children with unidentified pathogenic agents, the V3 and V68 regions of the 16S rRNA gene were amplified from fecal bacterial metagenomic DNA, separated on DGGE gel, and the dominant DNA bands were sequenced. As a result, the V3 and V68 regions of bacteria in persistent diarrheal children were less diverse and different from the corresponding DNA bands of the indicator strains. Sequence analysis and similarity comparison of six DNA bands of V3 regions and seven DNA bands of V68 regions showed that two V3 sequences (of 160 bp) derived from two samples were novel and did not match any genes from the non-redundant database, but they shared 93.75% similarity to each other. The four V3 sequences left derived from all three samples were the most similar (94.53-100%) with the corresponding genes of Brevibacterium. Six of the seven V68 sequences derived from dominant DNA bands of all three samples were the most similar (from 99.4% to 100%) to the corresponding genes of referent strains belonging to the genus Enterococcus. In sample D3, a sequence of the V68 region possessed 100% identity to the E. faecalis ATCC 19433 strain. This is the first study report that Brevibacterium was the dominant bacteria in the gastrointestinal microflora of children with persistent diarrhea although the bacterial genus has been reported to cause dangerous diseases in humans with immunodeficiency.
Isolation and characterization of beneficial bacteria from Apis Cerana honeybees from Hanoi, Vietnam
Authors: Quyen Dong Van , Lanh Pham Thi , Ha Thi Thu
Published: November 2024

Beneficial bacteria are vital for maintaining honeybee health by outcompeting pathogenic microorganisms, boosting immunity, and enhancing resilience to diseases. Identifying the specific bacterial strains associated with honeybees enables the development of targeted probiotics that can improve the health of bees and humans. The present study describes the isolation and identification of bacterial strains from Apis cerana honeybees in Hanoi, Vietnam, utilizing a culture-based method, Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry (MALDI-TOF) analysis, and 16S rRNA sequencing. MALDI-TOF analysis revealed several beneficial bacterial species, including Lactobacillus kunkeei, Lactobacillus plantarum, Pediococcus pentosaceus, Leuconostoc mesenteroides, Leuconostoc citreum, Bacillus subtilis, and Bacillus megaterium. Antimicrobial spectrum analysis showed that 16 out of the 23 identified isolates exhibited inhibitory effects against tested bacteria. Selected isolates with broad antimicrobial spectra, including L. kunkeei, L. plantarum, P. pentosaceus, L. mesenteroides, L. citreum, and B. subtilis, were further validated through 16S rRNA gene sequencing. The results confirmed the identity of these strains, emphasizing the probiotic potential of L. kunkeei, L. plantarum, L. mesenteroides, L. citreum, P. pentosaceus, and B. subtilis for honeybee health. Our findings provide valuable insights into the bacterial diversity and antimicrobial properties associated with honeybees, suggesting their use as probiotics in beekeeping and beyond.
Dichotomies of a Comic Text Analysis
Authors:   Perm State University, Rogotnev Ilya Yu.
Published: November 2024

The article deals with the poetics of the comic, the main focus is on the categories of analysis of individual comic segments. The material for the study was the prose of Nikolai Gogol. According to scientific literature, a comic work can be described not only as an aesthetic phenomenon but also as a cognitive construction and a special symbolic practice, which makes it possible to differentiate comic elements according to various criteria. A close reading of a comic fragment shows that a relatively small amount of text can contain many comic components, between which structural, content, and pragmatic differences can be detected. The comic text is considered through the prism of a set of dichotomies: active laughter – reduced laughter, satire – humor, comic deviation – nonsense, a simple comic element – a complex comic element. As shows an analysis of examples of reduced laughter (the term coined by M. M. Bakhtin), reduction of the comic is often associated with the loss (or incomplete realization) in the segment of special interdiscursivity. Grasping the dichotomy of satire and humor requires taking into account the diversity of intentions realized in satire: it can not only ridicule anomalous objects but also destroy the norm itself. Among the anomalies underlying the comic game, it is possible to distinguish deviations (shifts from the norm) and nonsense. A comic text based on nonsense requires special interpretive scenarios (scripts), in particular, it is proposed to distinguish between nonsense as such and absurdity. Distinguishing comic routines that are complex (in terms of their structure of expression) significantly complicates the issue of quantitative analysis of the comic: the volume of comic segments and the volume of comic effects are not fundamentally equivalent.
Representation of Values in Social Advertising
Authors:   Moscow City University, Kazachenko Oksana V. ,   Financial University under the Government of the Russian Federation
Published: November 2024

Values play an important role in human life, determining a person’s perception of the world, development, and behavior. The present research is in line with current trends: a search for sources of value formation as well as social construction of values at the state and municipal levels. These are necessary conditions for producing effective advertising that serves state policy on the preservation and strengthening of traditional Russian spiritual and moral values, which is especially important in the context of the collapse of value-based cultural stereotypes in the European space. The material for the study was collected from various media banks on the Internet by a continuous sampling method and further analyzed with the use of descriptive-analytical and descriptive-comparative research methods. The author notes the prevalence of personal values over public ones, which must be considered in the creation of social advertising. The novelty of the approach lies in the analysis of social advertising as one of the factors in the formation of a personal value system. The main purpose of social advertising is to identify social problems and ways to solve them, often using the contrast of values and anti-values. Social advertising includes many types, such as anti-terrorist, family, intergenerational, health and healthy lifestyle, security advertising. The features of social advertising are a combination of verbal and nonverbal (audiovisual) components as well as the use of antithesis and rhyming slogans, which is realized in the opposition ‘value – anti-value’. The use of a suggestive potential of poetic text makes it possible to construct a ‘correct’ model of behavior in socially significant situations. The use of an antithesis corresponds to two types of fragments of the represented reality: problematic and ideal. The author concludes that social advertising contributes to the formation of a certain value sphere, which is part of the image of the world as perceived by a person.
Titelei/Inhaltsverzeichnis
Authors:   Autor(en)
Published: November 2024

Abstract
«Auf keinen Fall ein Violett!»
Authors: Hartwig: Ina
Published: November 2024

Es dauert eine ganze Weile, bis Siegfried Unselds Wunsch in Erfüllung geht. Er will Ingeborg Bachmann vom Piper Verlag weglocken und zu seiner Autorin machen. Ihren ersten Auftritt bei Suhrkamp hat sie 1961 zunächst als Übersetzerin von Ungarettis Gedichten. Richtig glücklich aber wird Unseld erst sein, wenn mit Malina ihr erster eigener literarischer Titel in seinem Programm erscheint. Der Roman kommt im März 1971 auf den Markt, der Verleger schaltet mehrere überregionale Anzeigen, darunter erstmals eine im Spiegel, was ihm 7500,– DM wert ist. Und wie sich zeigen wird, zahlt sich das Geschäft aus.
Der unbedingte Unseld
Authors: Grünbein Durs
Published: November 2024

Unselds Faust Unseld ballte die Faust eine rechte hatte er und eine linke Faust selten trug er sie war er aufgebracht offen geballt Unseld haßte Gewalt er war Unternehmer sein Produkt war das Buch unbändig aktiv war er Unselds Ungeduld war legendär man unkte er habe oft in den Taschen die Fäuste geballt alle beide
Venedig, Herbst 1989
Authors: Cammann: Alexander
Published: November 2024

1989 war das große Partyjahr der geistigen Bundesrepublik. Denn ihr stilprägender Jahrgang 1929 feierte seinen 60. Geburtstag, und das nicht gerade bescheiden. Der 29-jährige Frank Schirrmacher, als Nachfolger Marcel Reich-Ranickis frischgebackener Literaturchef der F.A.Z. , hatte gleich am 2. Januar programmatisch zur Amtseinführung die kommenden Jubilare, von Walter Kempowski, Christa Wolf, Heiner Müller, Peter Rühmkorf, Michael Ende bis zu den Suhrkamp-Autoren Hans Magnus Enzensberger und Jürgen Habermas, hochleben lassen und sie zugleich frech in die Vergangenheit geschoben.
Das Geisterhaus. Phänomenologie einer Villa im Westend
Authors: Maak Niklas
Published: November 2024

Im Juni dieses Jahres verschickte das internationale Maklerbüro Engel & Völkers ein Exposé, mit dem eine «repräsentative Villa im Frankfurter Holzhausenviertel» beworben wurde. Für 4,15 Millionen Euro sei die «renommierte Villa Unseld» mit 13 Räumen und 572 qm Nutzfläche zu kaufen; einige Zimmer seien «bis zur Decke mit Bücherregalen ausgestattet». Der Pfeil im mitgelieferten Energieausweis des Gebäudes steht bei H im roten Bereich. Ein Detail schien den Maklern offenbar zeigenswert: An einer Wand pinnt Adornos Satz «Es gibt kein richtiges Leben im falschen», jemand hat «Es gibt kein falsches Leben im richtigen» darüber geklebt, was den Übergang schon vorwegzunehmen scheint von der kritischen Theorie, die hier eines ihrer Zuhause fand, zum robust-pragmatischen Maklerhumor einer Stadtgesellschaft, die Räume vor allem als Real Estate Assets wahrnimmt. Wie begann das, was auf der Schlachtbank des Immobilienmarkts endet und wofür stand dieser Ort, der jetzt nur noch ein Haus mit schlechten Energiewerten ist?
Rheinischer Katholizismus und ästhetische Form. Fleckhaus’ Kunst: Wie Suhrkamp schön wurde
Authors: Illies Florian
Published: November 2024

Violett ist die Farbe, mit der der Regenbogen beginnt. Violett ist die Farbe, mit der das Kirchenjahr beginnt. Violett musste also auch die Farbe sein, mit der jener Regenbogen seinen Anfang nimmt, den Willy Fleckhaus über das geistige Firmament eines transzendental obdachlos gewordenen Nachkriegsdeutschlands spannte. Zum Trost und als Verheißung. Als der gläubige Katholik Fleckhaus, der seine ersten journalistischen Erfahrungen 1947 beim christlichen Magazin Der Fährmann in Freiburg sammelte, im Jahre 1963 die neue säkulare Religion des katholischen Verlegers Siegfried Unseld erstmals in sattem, vorösterlichem Violett entfaltete, dann natürlich als Umschlag eines Buches, in dem es um den existenziellen Kampf um die Lufthoheit über den Beichtstühlen und Schreibtischen geht: mit Brechts Leben des Galilei.
Formulación de proyectos sociales y atributos para el liderazgo. Miradas desde dirigentes sociales chilenos
Authors: Cárcamo Vásquez Héctor , Gallegos Fuentes Marcelo Andrés , Jarpa Carmen Gloria , Leaman Hasbún Sady David Alejandro , Vargas Muñoz Héctor Agustín
Published: November 2024

Objetivo. El trabajo busca describir las valoraciones que dirigentes sociales chilenos otorgan a tres dimensiones relevantes para la formulación y ejecución de proyectos sociales (conocimientos, la comunidad y los agentes implicados). Asimismo, se busca identificar los atributos que consideran relevantes para el ejercicio del liderazgo en este tipo de iniciativas. El colectivo participante corresponde a 741 dirigentes sociales de la Región de Ñuble, emplazada en la zona centro sur de Chile. Metodología. El estudio es cuantitativo de alcance descriptivo. La información fue recogida por medio de una encuesta ad hoc. Resultados. Los resultados evidencian una alta valoración en la dimensión conocimientos conceptuales, procedimentales y prácticos. En la dimensión participación de la comunidad se aprecia la valoración en todos los niveles (informativo, participativo, colaborativo y comprometido). En la dimensión agentes implicados, la valoración se expresa en orden descendente (Estado, comunidad, municipio, expertos y empresa privada). En cuanto a los atributos considerados relevantes para el ejercicio del liderazgo, destacan los de carácter democrático tales como el respeto por las opiniones y el establecimiento del dialogo para llegar a acuerdos. Conclusiones. Finalmente, se evidencia la necesidad de realizar estudios que aborden desde una perspectiva cualitativa los atributos que los dirigentes consideran relevantes para el ejercicio del liderazgo.
Reflexões preliminares sobre a apreensão do trabalho na categoria profissional de assistentes sociais
Authors: Zacarias Inez Rocha , Gomes de Carvalho Aquino Isaura , Escurra María Fernanda
Published: November 2024

O artigo em questão apresenta os resultados preliminares de uma pesquisa em desenvolvimento, sendo executada no âmbito de uma pesquisa internacional. A análise é situada na área dos fundamentos históricos e teórico-metodológicos do Serviço Social e propõe recuperar a leitura e o debate histórico-crítico sobre o trabalho profissional, inaugurado por Marilda Villela Iamamoto. O texto concentra sua atenção na identificação de como a categoria trabalho é apreendida pela categoria de assistentes sociais, considerando o debate sobre o duplo caráter que o trabalho historicamente assume sob o capital: trabalho concreto e trabalho abstrato. Para isso, com base na definição prévia de descritores para a coleta de dados, são apresentados avanços da pesquisa que resultam da análise do conteúdo de trabalhos publicados nos Anais do XVII Congresso Brasileiro de Assistentes Sociais (CBAS), evento de referência para a categoria profissional no Brasil, realizado virtualmente em 2022.
Aproximações entre o Serviço Social do Reino Unido e do Brasil na contestação ao Serviço Social Tradicional
Authors: Defilippo Antoniana , De O. Ferreira Camila Caroline , Canêo Giovanna , Silva Pedro Gabriel , Pereira de Souza Oliveira Shirleny
Published: November 2024

Este artigo objetiva apresentar algumas aproximações entre o Serviço Social do Brasil e do Reino Unido - especialmente no que diz respeito à contestação ao Serviço Social Tradicional. Para tanto, dialoga com as principais produções bibliográficas que versam sobre os determinantes históricos que compõem a emergência da perspectiva contestatória ao Serviço Social Tradicional nestes dois países, no contexto das relações sociais capitalistas entre as décadas de 1970 e 1990, destacando as dimensões teórico-metodológica, técnico-operativa e ético-política. Conclui-se que o diálogo entre as vertentes críticas do Serviço Social dos dois países mostra-se promissor e, por isso, merece ser adensado.
El anticapitalismo y sus aproximaciones al trabajo social brasileño: anunciando algunas controversias y concepciones
Authors: Rodrigues Melatti Kelly , De Fátima Rodrigues Terezinha , Yazbek Maria Carmelita
Published: November 2024

Este artículo aborda algunas controversias teóricas sobre las concepciones que involucran al anticapitalismo en sus múltiples formas de expresión y significados ideopolíticos en la teoría social crítica, y presenta –de manera aproximada– la particularidad del trabajo social brasileño en el contexto de las luchas anticapitalistas. El texto se desarrolló a partir de estudios y reflexiones teóricas basadas en autores (as) que problematizan el anticapitalismo en la dimensión teórico-crítica. El anticapitalismo está presente en el trabajo social y en el proyecto profesional de esta profesión en Brasil, articulado a un proyecto societal en el campo de la emancipación humana. Son expresiones de esta presencia el vínculo orgánico con los movimientos y las luchas sociales de la sociedad brasileña, extremadamente desigual, marcada por el capitalismo tardío, dependiente y subordinado; y el esclavismo colonial que se ha reconfigurado en prácticas racistas y patriarcales. Este es el terreno fértil de las luchas anticapitalistas, y el trabajo social en Brasil no se ha apartado de esta inserción ético-política, a pesar de estar permeado por tensiones y contradicciones.
The Mediating Role of Collaborative Climate in the Relationship between Shared Instructional Leadership and School Effectiveness: A Structural Equation Modeling
Authors: Demir Muhammed , Dilekçi Ümit
Published: November 2024

This study investigated the mediator role of collaborative climate in the relationship between shared instructional leadership and perceived school effectiveness based on teachers’ perceptions adopting a structural equation model. The research universe included 402 physical education teachers working in Batman (Türkiye) in the 2022-2023 academic year. Since the researchers aimed to reach all the teachers in the population (N=402), they did not employ a sampling method. The participants were 272 physical education teachers, with a response rate of 67.66%. Pearson correlation coefficients were calculated to reveal the associations among study variables. The findings revealed statistically significant, positive, and high-level correlations among variables. On the other hand, a structural equation model was used to test the hypotheses. The findings suggested that shared instructional leadership had a significant effect on collaborative climate and but not on perceived school effectiveness, and the effect of collaborative climate on perceived school effectiveness was also statistically significant. Lastly, collaborative climate mediated the relationship between shared instructional leadership and perceived school effectiveness. The findings were discussed based on the previous literature, and suggestions were brought for practitioners and researchers.
Inductive and Deductive Reasoning in Byrom vs. Johns Hopkins Bayview Hospital
Authors: Howard Smith
Published: November 2024

The status quo that results from medical malpractice litigation is 85,000 medical malpractice lawsuits filed per year, of which 66% are potentially frivolous. There are also 3 million claims but only 85,000 are represented. There is something wrong with this status quo, which prompts questions about traditional decision-making Traditional decision-making is inductive reasoning. Deductive reasoning is hypothesis testing. The objective evidence and the burden of proof are the same in both; except hypothesis testing has a greater level of confidence. The differences are examined in detail. Of the parties involved in dispute resolution, medical experts are essential, medical experts are doctors. Doctors are familiar with hypothesis testing and threats to validity. Doctors, who are medical experts, are duty-bound to be objective. Hypothesis testing best satisfies this duty. Nothing prohibits medical experts from using hypothesis testing when they review a case to arrive at an opinion; although, until now, none do. Yet, as doctors, it is expected of them and they can never be prohibited from doing so. In the final analysis, traditional decision-making subjectively infers a departure from the standard of care; however, hypothesis testing objectively proves it.
Pensiero giuridico e linguaggio cinematografico. Il falso capitano di Köpenick e lo sguardo dell'Altro
Authors: Gambino Francesco
Published: November 2024

Il saggio, che intende indagare gli intrecci e le commistioni tra pensiero giuridico, filosofia e linguaggio cinematografico, si propone di ridurre a modelli descrittivi i vari fenomeni trattati allo scopo di rilevare similarità strutturali tra di essi. È una ricerca interdisciplinare volta ad evidenziare l’idea che l’attività umana, che si manifesti in costruzioni teoriche, pratiche o artistiche, muove sempre dall’uni-totalità della persona ed è atto di invenzione e di produzione di forme. Cinema, diritto, filosofia si ritrovano sulla stessa strada perché hanno di mira il medesimo oggetto: il comportamento umano. Può mostrarsi in ragione della narrazione di una storia, di uno studio di psicologia o di una terapia, delle indagini sulla intersoggettività o in funzione di una regola giuridica. In questo scenario, cinema e diritto condividono tra loro e con altre pratiche il destino empirico della nostra evoluzione culturale.
Endüstri 5.0'a Geçişte Siber Güvenlik: Yeni Sanayileşen Ülkeler Üzerine Bir İnceleme
Authors: Duran Zafer
Published: November 2024

Bu çalışmanın amacı, yeni sanayileşen ülkelerin siber güvenlik düzeylerini derinlemesine değerlendirerek, bu ülkelerin Endüstri 5.0'a geçişlerini güvenli bir biçimde gerçekleştirmelerine yönelik içgörüler sunmaktır. Endüstri 5.0, kişiselleştirilmiş ürün ve hizmetlerin sürdürülebilir bir biçimde sunulmasını sağlayarak dijital dönüşümde yeni bir aşamayı temsil etmekte, ancak bu süreçte ortaya çıkan siber güvenlik riskleri işletmeler için önemli tehditler oluşturmaktadır. İşletmeler bu risklere karşı gerekli önlemleri alsalar da siber güvenliğin etkin bir şekilde sağlanabilmesi ülkelerin benimsediği politikalarla yakından ilişkilidir. Bu nedene ülkelerin siber güvenliğe ilişkin yaklaşımları, Endüstri 5.0’a geçiş sürecini önemli ölçüde şekillendirmektedir. Bu bağlamda, Entropi tabanlı MABAC yöntemiyle yapılan değerlendirme, organizasyonel önlemlerin yeni sanayileşen ülkeler için en kritik siber güvenlik göstergesi olduğunu ve Malezya'nın bu ülkeler arasında siber güvenlik düzeyi bakımından lider konumda olduğunu ortaya koymuştur.
XIX. Yüzyılda Avrupalı Seyyahların Gözünden Tebriz
Authors: Bal Gülten , Soofizadeh Abdolvahid
Published: November 2024

XIX. yüzyılda Kaçar Devleti hâkimiyeti altındaki İran toprakları ve dolayısıyla Azerbaycan Eyaleti, siyasî, askerî ve ekonomik gelişmeler etkisiyle Rusya ve Avrupa’nın dikkatini fazlasıyla cezbetmiştir. Tarihî İpek Yolunun güzergâhında bulunan ve ticarî malların Asya, Osmanlı toprakları ve Avrupa arasında taşındığı yolun bir durağı konumundaki Tebriz şehri, diğer yandan veliaht makamı olması nedeniyle siyasî olarak da bir merkez konumundaydı. Bu yüzyılda siyasî, ekonomik, askerî, ticarî vb. sebeplerle birçok yabancı İran topraklarına seyahatler düzenlemiş ve bunun sonucunda edindikleri izlenimleri aktarmışlardır. Çalışmamızda, XIX. yüzyılda çeşitli gerekçelerle İran ve Azerbaycan coğrafyasını ziyaret eden ve bu geziler esnasında gördüklerini, yaşadıklarını kaleme alan bazı yabancı seyyahların anlatımlarında, İran’ın metropollerinden biri olan Tebriz şehrinin profilini çizmek amaçlanmıştır.
İstanbul’da Kent Tarımını Tartışmak: Sermaye ve Topluluk Temelli Yaklaşımlar
Authors: Yıldızcan Cemil
Published: November 2024

Bu makale, “gıdayı engelleyen” (Tornaghi, 2017) bir megakent olan İstanbul’daki kent tarımı uygulamalarının dönüştürücü ve güçlendirici boyutlarını eleştirel bir perspektifle tartışıyor. Çalışma, kent tarımının içsel çelişkilerini ve çatışmalarını (McClintock, 2014) neoliberal kentsel bağlamda yerlerinden edilen geleneksel tarım aktörleri ve ilişkileri üzerindeki etkileri bağlamında tartışıyor. Ayrıca, giderek daha fazla kamusal ve özel teşviklerden yararlanan topraksız ve dikey tarım uygulamalarının, toplumsal olarak gömülü geleneksel tarım uygulamalarını ve gıdayı müşterekleştirme imkanlarını yerinden edip etmediğini sorguluyor. Bu bağlamda, sermaye yoğun yeni kent tarımı uygulamalarının, neoliberal bireyselleşme ve piyasalaştırmaya meydan okuma potansiyelinin kısıtlı olduğunu iddia ederken, kentsel gıda müşterekleri söylemi içinde yeniden konumlandırılabilecek, kolektif kaynaklar ve topluluk temelli sorumluluk aracılığıyla neoliberal ilişkilerin sömürgeleştirdiği alanlarda toplulukları özgürleştirici ve güçlendirici bazı yanlar barındırdığının da altı çiziliyor.
Una Reseña del libro Queering the Museum, de Nikki Sullivan y Craig Middleton
Authors:   Universidad de Filipinas Diliman, Tiñana Banquiles Venes Carmelo
Published: November 2024

Queering the Museum de Nikki Sullivan y Craig Middleton critica los marcos heteronormativos en los museos y propone una ética queer que trascienda la inclusividad. Las autoras argumentan que los museos deben ser espacios dinámicos de discurso crítico y cambio social, influidos por sus contextos socioculturales. Usando las teorías posestructuralistas y posthumanistas, destacan la importancia de descentralizar la agencia humana, viendo los objetos del museo como agentes interconectados. El libro critica la baja representación LGBTQI+ y ofrece estrategias para crear prácticas museísticas más inclusivas, transformando los museos en espacios colaborativos y socialmente transformadores.
APPLICATION OF THE BACTERIAL OPTIMIZATION METHOD TO MINIMIZE THE COST OF CONDUCTING A MULTIFACTOR EXPERIMENT
Authors:   National Aerospace University ", Koshevoy N.D. ,   Kharkiv Aviation Institute",   , Kharkiv, Ukraine, Pylypenko O.T. ,   National Aerospace University "Kharkiv Aviation Institute", Kharkiv, Ukraine
Published: November 2024

Experiment planning allows you to get a mathematical model in a time and resource restricted conditions. When conducting experimental research in industry, methods of optimizing production processes are used. The order of the levels’ alternation of production processes’ change has a strong influence on the time of the experiment. Most often, experiments are multifactorial and associated with the search for optimal conditions for the experiment, the selection of the most rational equipment. At the same time, there is an urgent need to improve the efficiency of experimental research. There are many factors to consider when implementing a project. The main tasks are to use the initial data, carefully study them with a minimum amount of observations and information, study the 31 influence of individual factors and calculate the most effective procedure for performing the experiment. One of the main tasks is to obtain the maximum amount of information that is useful to us, so that when we re-examine, we can take into account the previous results and use this data to increase efficiency and reduce the time spent in subsequent experiments. One of the advantages is the versatility of the method and the possibility of applying it in a large number of research areas. The purpose of this article is to further develop the methodology of time-optimal experiment planning. Therefore, there is a need to increase the level of research efficiency with a minimum investment of time and resources. From the reasons listed above, we see that there is an urgency to automate and optimize experiments. Based on this, a bacterial optimization method was developed for matrix optimization. The analysis of known methods of synthesis of time-optimal experimental plans was carried out. The effectiveness of the bacterial method is shown in comparison with the original experimental outline, analysis of permutations and random search. Object of research: planning processes of multifactor experiments in conditions of limited time and resources. Subject of research: application of the bacterial optimization method to build a mathematical model with the optimal order of the experiment.
FEATURES OF SIMULATION OF A RAMAN AMPLIFIER BASED ON A DISPERSION COMPENSATING FIBER WITH UNIFORM GAIN BAND IN C+L TELECOMMUNICATION WINDOWS
Authors:   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, Korchak O.V. , Krutin Y.V. ,   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
Published: November 2024

The results of the modeling of a broadband optical fiber Raman amplifier (FRA) with a multi-wavelength pumping and a uniform gain band covering C+L telecommunication windows in an active fiber with compensation of dispersion type DCF are presented. It has been demonstrated that the SRS gain profile, that is, the distribution of the optical radiation gain over the frequency range of the Stokes shift in Raman scattering, is the main characteristic of the FRA. Optimum values of the parameters of the pump sources are presented, namely spectral distribution of wavelengths and relative power of all pump sources, at which it is possible to obtain a minimum non-uniformity of the gain band. Based on the results of the calculations, a minimum non-uniformity of the SRS gain profile on almost the whole operating range was obtained for 6 pumping sources. It is shown that the analytical form of the gain profile greatly simplifies the procedure of modeling of the main gain parameters for FRA with multi-wavelength pumping for 3-6 wavelengths of pump sources. Furthermore, the proposed simplified analytical model makes it possible to quickly obtain theoretical estimates of the main parameters of the operating wavelength band of FRA with an arbitrary number of pumping lasers in the multiwave pump source.
Multiple morcelloma of the abdominal cavity: a case report
Authors: Tikhomirov A. L. , Kazenashev V. V. , Maminova M. V. , Davydenko N. L. , Rurua N. V. , Alieva Z. G.
Published: November 2024

Introduction. Uterine fibroids are benign monoclonal hormone-sensitive tumors arising from smooth muscle cells of the cervix or body uterus. Myomectomy has become a treatment «golden standard» upon transition to the strategy of organ-preserving surgery. The introduction of morcellation into wide clinical practice allowed to expand the indications for laparoscopic myomectomy, which is usually much better tolerated by patients due to lower invasiveness, short period of rehabilitation and low blood loss. However, the use of morcellators along with the obvious advantages has also specific complications particularly emergence of iatrogenic parasitic fibroids of the abdominal cavity upon improper surgery technique.

Aim: analysis of a clinical case of multiple morcelloma.

Case presentation. Female patient I., 57 years old, underwent laparoscopic myomectomy in 2003. In 2023 during a routine examination at the Central Clinical Hospital «RZD-Medicine» multiple asymptomatic iatrogenic fibroids of the abdominal cavity were revealed. The patient underwent planned surgical intervention to remove all abnormal masses.

Results. During the revision upon surgical intervention, a node of 1.5x1.5 cm was found on the parietal peritoneum along the left anterior wall, as well as a node of 1.5x1.5 cm on the parietal peritoneum along the right anterior wall. There was also observed a 2x3 cm node at the edge of the omentum. In the left and right side of peritoneum of the sacro-uterine ligament, there were found nodes of up to 1.0 cm in size. In the area of the small intestinal mesentery, a dense node of 4.5 cm was observed 70 cm away from the ileocecal valve. Due to small size, all the fibroids were extirpated and removed from the abdominal cavity without morcellation, complete hemostasis was performed. No complications were noted during postoperative period.

Conclusion. Iatrogenic fibroids are quite rare but nevertheless a potential complication of laparoscopic myomectomy. Gynecologists should pay special attention to follow proper technique upon surgical intervention. Morcellation and subsequent extraction of fibroids should be performed strictly with use of airtight container to avoid ingress of myomatous tissue into the abdominal cavity followed by emergence of iatrogenic fibroids.
Özel Nitelikli Uluslararası Sermaye Hareketleri ile Finansal Gelişmişlik Arasındaki İlişki: Türki Cumhuriyetler İncelemesi
Authors: Saka Ilgın Kübra
Published: November 2024

Doğrudan yabancı sermaye yatırımları ve portföy yatırımlarını içeren uluslararası sermaye hareketleri, gelişmekte olan ülkeler için önemli bir yabancı sermaye kaynağı olmaya devam etmektedir. Bu çalışma, Türki Cumhuriyetlerden Türk Devlet Teşkilatı üye ülkelerinin doğrudan yabancı sermaye yatırımları ile portföy yatırımlarının finansal gelişmişilikleri ile ilişkisinin incelenmesini amaçlamaktadır. Değişkenlerin yapısı analiz yöntemi olarak zaman serisi analizlerinden ARDL Sınır testi, Johansen-Juselius Eşbütünleşme testi, Toda-Yamamoto Nedensellik testlerini kullanmayı gerekli kılmıştır. Uygulanan analizler sonrasında bu ülkelerin genelinde doğrudan yabancı sermaye yatırımları ve/veya portföy yatırımları ile ülkelerin yerli finansal gelişmişlikleri arasında uzun dönemde pozitif anlamlı ilişkiler olduğu gözlenmiştir. Kısa dönemli sapmaların ise uzun dönemde dengeye ulaştığı belirlenmiştir. Ayrıca finansal gelişmişlikten doğrudan yabancı sermaye yatırımları ve/veya portföy yatırımlarına doğru tek yönlü nedensellik ilişkisi olduğu dolayısıyla da finansal gelişmişliğin uluslararası sermaye hareketlerini olumlu yönde etkilediği ifade edilebilmektedir. Çalışmanın hem yatırımcılar hem de politika yapıcılara fayda sağlayabileceği düşünülmektedir.