bluuebunny/crossref_metadata_2025 huggingface dataset:
The Type of Conservative Management Could Be Related to the Strength of the Inspiratory Muscles of Adolescents with Idiopathic Scoliosis—A Case Series
Authors: León-Morillas Felipe , de Oliveira-Sousa Silvana Loana , Andrade-Ortega Juan Alfonso , Ibáñez-Vera Alfonso Javier , Lomas-Vega Rafael , Zagalaz-Anula Noelia
Published: November 2021

Adolescent idiopathic scoliosis (AIS) is a lateral curvature of the spine with a Cobb angle of at least 10° with an unknown etiology. It is recognized that AIS may affect respiratory function. This study aims to describe and compare respiratory function in a case series of patients with scoliosis who underwent different types of therapeutic management: no intervention, orthotic brace, and global postural reeducation (GPR). Fifteen AIS patients were included in this study (seven no intervention, four orthotic brace and four GPR). Lung function and inspiratory muscle strength were measured and analyzed, as well as sociodemographic, clinical, and anthropometric variables. Significant correlations were observed between height (cm) and maximum inspiratory pressure (MIP) reference (cmH2O) and forced vital capacity (FVC) (liters) (r = 0.650 and r = 0.673, respectively; p < 0.01); weight (Kg) and MIP reference (cmH2O) (r = 0.727; p < 0.01); and Main curve degrees (Cobb angle) and FVC% (r = −0.648; p < 0.01). The AIS cases that underwent GPR treatment presented a greater MIP (% predictive) compared to the no intervention and brace cases (201.1% versus 126.1% and 78.4%, respectively; p < 0.05). The results of this case series show a possible relation whereby patients undergoing treatment with the GPR method have greater inspiratory muscle strength compared to the no intervention and brace cases. Studies with larger samples and prospective designs must be performed to corroborate these results.
The Association between the Concentration of Heavy Metals in the Indoor Atmosphere and Atopic Dermatitis Symptoms in Children Aged between 4 and 13 Years: A Pilot Study
Authors: Choi Hyun Seung , Suh Michelle J. , Hong Sung Chul , Kang Ju Wan
Published: November 2021

Background: A correlation between the harmful effects of air pollutants and atopic dermatitis has been reported. There are few studies on the correlation between the concentration of heavy metals in the indoor atmosphere and symptoms of atopic dermatitis. Methods: Twenty-two homes of children showing atopic dermatitis symptoms were enrolled, and eighteen homes with similarly aged children without symptoms or a history of atopic dermatitis participated as a control group. We measured the concentrations of various air pollutants (particulate matter 10, carbon dioxide, carbon monoxide, formaldehyde, nitrogen dioxide, volatile organic compounds (VOCs), ozone, radon, bacterial aerosols, and mold) as well as various heavy metals, such as lead, cadmium, and mercury, in the living room and children’s bedroom of each home. Results: Lead was more commonly detected in the indoor air in houses of children with atopic dermatitis (15/22) as compared to in the control group (3/18) (chi square test, p = 0.002). In adjusted logistic regression analysis, VOCs and lead were significantly associated with atopic dermatitis (p < 0.05). Conclusion: Our study shows that lead in indoor air might be associated with atopic dermatitis, even if the concentrations of airborne lead are below the safety levels suggested by health guidelines.
GI-SleepNet: A Highly Versatile Image-Based Sleep Classification Using a Deep Learning Algorithm
Authors: Gao Tianxiang , Li Jiayi , Watanabe Yuji , Hung Chijung , Yamanaka Akihiro , Horie Kazumasa , Yanagisawa Masashi , Ohsawa Masahiro , Kume Kazuhiko
Published: November 2021

Sleep-stage classification is essential for sleep research. Various automatic judgment programs, including deep learning algorithms using artificial intelligence (AI), have been developed, but have limitations with regard to data format compatibility, human interpretability, cost, and technical requirements. We developed a novel program called GI-SleepNet, generative adversarial network (GAN)-assisted image-based sleep staging for mice that is accurate, versatile, compact, and easy to use. In this program, electroencephalogram and electromyography data are first visualized as images, and then classified into three stages (wake, NREM, and REM) by a supervised image learning algorithm. To increase its accuracy, we adopted GAN and artificially generated fake REM sleep data to equalize the number of stages. This resulted in improved accuracy, and as little as one mouse’s data yielded significant accuracy. Due to its image-based nature, the program is easy to apply to data of different formats, different species of animals, and even outside sleep research. Image data can be easily understood; thus, confirmation by experts is easily obtained, even when there are prediction anomalies. As deep learning in image processing is one of the leading fields in AI, numerous algorithms are also available.
Special Issue “Current and Evolving Practices in the Quality Control of Cosmetics”
Authors: Dodou Kalliopi
Published: November 2021

Quality Control (QC) testing of Cosmetic personal care and fragrance products is a key part of the products’ launch to the market [...]
Adsorption and Release Characteristics of Purified and Non-Purified Clinoptilolite Tuffs towards Health-Relevant Heavy Metals
Authors: Haemmerle Mona M. , Fendrych Joachim , Matiasek Elisabeth , Tschegg Cornelius
Published: November 2021

The occurrence of health-relevant contaminants in water has become a severe global problem. For treating heavy-metal-polluted water, the use of zeolite materials has been extended over the last decades, due to their excellent features of high ion exchange capacity and absorbency. The aim of this study was to assess the effect of heavy metal uptake of one purified (PCT) and two non-purified clinoptilolite tuffs (NPCT1 and NPCT2) in aqueous solutions on monovalent ions Ni+, Cd+, Cs+, Ba+, Tl+, and Pb+. Experiments were furthermore carried out in artificial gastric and intestinal fluids to mimic human digestion and compare removal efficiencies of the adsorbent materials as well as release characteristics in synthetic gastric (SGF) and intestinal fluids (SIF). Batch experiments show low sorption capacities for Ni+ and Cd+ for all studied materials; highest affinities were found for Ba+ (99–100%), Pb+ (98–100%), Cs+ (97–98%), and Tl+ (96%), depending on the experimental setup for the PCT. For the adsorption experiments with SGF, highest adsorption was observed for the PCT for Pb+, with an uptake of 99% of the lead content. During artificial digestion, it was proven that the PCT did not release Ba+ cations into solution, whereas 13,574 ng·g−1 and 4839 ng·g−1 of Ba+ were measured in the solutions with NPCT1 and NPCT2, respectively. It was demonstrated that the purified clinoptilolite tuff is most effective in remediating heavy-metal-polluted water, particularly during artificial digestion (99% of Pb+, 95% of Tl+, 93% of Ba+). In addition, it was shown that the released amount of bound heavy metal ions (e.g., barium) from the non-purified clinoptilolite tuffs into the intestinal fluids was significantly higher compared to the purified product.
The Role of an Anti-Inflammatory Diet in Conjunction to COVID-19
Authors: Ling Victoria , Zabetakis Ioannis
Published: November 2021

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), otherwise known as COVID-19, has challenged healthcare systems at an international level. COVID-19 suppresses the immune system by causing a systemic inflammatory response, also known as cytokine release syndrome, leaving COVID-19 patients with high levels of proinflammatory cytokines and chemokines. Nutrition’s function in the respiratory and immune systems has been investigated in much research, and its significance cannot be overstated, as the nutritional status of patients has been shown to be directly connected with the severity of the disease. Key dietary components such as vitamin C, D, omega-3 fatty acids, and zinc have shown potential in their anti-inflammatory effects, as well as the famous Mediterranean diet. This review aims to discuss the use of anti-inflammatory dietary approaches to prevent Sars-CoV-2 or lessen COVID-19 effects.
Stem Cell Therapy for Post-Traumatic Stress Disorder: A Novel Therapeutic Approach
Authors: Gala Dhir , Gurusamy Vikram , Patel Krishna , Damodar Sreedevi , Swaminath Girish , Ullal Gautam
Published: November 2021

Stem cell therapy is a rapidly evolving field of regenerative medicine being employed for the management of various central nervous system disorders. The ability to self-renew, differentiate into specialized cells, and integrate into neuronal networks has positioned stem cells as an ideal mechanism for the treatment of epilepsy. Epilepsy is characterized by repetitive seizures caused by imbalance in the GABA and glutamate neurotransmission following neuronal damage. Stem cells provide benefit by reducing the glutamate excitotoxicity and strengthening the GABAergic inter-neuron connections. Similar to the abnormal neuroanatomic location in epilepsy, post-traumatic stress disorder (PTSD) is caused by hyperarousal in the amygdala and decreased activity of the hippocampus and medial prefrontal cortex. Thus, stem cells could be used to modulate neuronal interconnectivity. In this review, we provide a rationale for the use of stem cell therapy in the treatment of PTSD.
MYCN in Neuroblastoma: “Old Wine into New Wineskins”
Authors: Braoudaki Maria , Hatziagapiou Kyriaki , Zaravinos Apostolos , Lambrou George I.
Published: November 2021

MYCN Proto-Oncogene, BHLH Transcription Factor (MYCN) has been one of the most studied genes in neuroblastoma. It is known for its oncogenetic mechanisms, as well as its role in the prognosis of the disease and it is considered one of the prominent targets for neuroblastoma therapy. In the present work, we attempted to review the literature, on the relation between MYCN and neuroblastoma from all possible mechanistic sites. We have searched the literature for the role of MYCN in neuroblastoma based on the following topics: the references of MYCN in the literature, the gene’s anatomy, along with its transcripts, the protein’s anatomy, the epigenetic mechanisms regulating MYCN expression and function, as well as MYCN amplification. MYCN plays a significant role in neuroblastoma biology. Its functions and properties range from the forming of G-quadraplexes, to the interaction with miRNAs, as well as the regulation of gene methylation and histone acetylation and deacetylation. Although MYCN is one of the most primary genes studied in neuroblastoma, there is still a lot to be learned. Our knowledge on the exact mechanisms of MYCN amplification, etiology and potential interventions is still limited. The knowledge on the molecular mechanisms of MYCN in neuroblastoma, could have potential prognostic and therapeutic advantages.
Vitamin D and Its Role in Oral Diseases Development. Scoping Review
Authors: Diachkova Ekaterina , Trifonova Daria , Morozova Elena , Runova Gyuzel , Ashurko Igor , Ibadulaeva Maria , Fadeev Valentin , Tarasenko Svetlana
Published: November 2021

Vitamin D is a fat-soluble secosteroid that plays a significant role in the whole body, including the maxillofacial region. The discovery of its receptors in many cells and organs made it possible to reveal the participation of vitamin D not only in the regulation of calcium phosphate metabolism, but also in immune processes, in providing anti-inflammatory and antimicrobial effects, slowing down cell proliferation and stimulating differentiation. In this literature review, we demonstrate the association between low vitamin D levels and the development of recurrent aphthous stomatitis, the course and response to treatment of squamous cell carcinoma of the oral cavity, the severity of periodontal diseases, and the processes of osseointegration and bone remodeling during dental implantation and guided tissue regeneration. The aim of our article was to demonstate a possible connection between vitamin D level and the oral diseases that can be presented at an oral surgery appointment, which will help clinicians to reduce the risk of early dental implant failure, ensure favorable outcomes of augmentative operations, as well as decrease the destructive effects of severe periodontitis and other conditions throug knowledge and timely lab tests and endocrinologist prescriptions.
Epidermal Protein C Levels Correspond to Local Injury Severity and Increased Clinical Support in Burn Patients
Authors: Zhao Ruilong , Wang Duo , Lang Thomas Charles , Kim Albert , Wijewardana Aruna , Vandervord John , McGrath Rachel , Fulcher Gregory , Lin Haiyan , Xue Meilang , Jackson Christopher John
Published: November 2021

The protein C (PC) system has proven to be a crucial cascade in systemic inflammatory and coagulopathic disorders such as severe sepsis and, more recently, in severe burns. We aimed to conflate our recent systemic findings with further investigations in the local tissue effects of a severe burn injury on the expression of PC and its main receptor endothelial protein C receptor (EPCR). Of the 86 patients enrolled in our recent study, 34 consented to biopsies of both normal and burn edge tissue. These were examined histologically and immunostained for PC, EPCR, and CD68. The burn samples expressed lower PC (p = 0.0027) and higher EPCR (p = 0.0253) than the normal samples in a histological severity-dependent manner. There was also a negative association between PC expression and CD68 positive macrophage infiltration (τb = −0.214, p = 0.020), which was expectedly higher in burn edge samples (p < 0.0005). Interestingly, while there were no correlations between tissue and plasma PC or EPCR, local PC expression was also prognostic of our previously established outcome of a patient requiring increased medical support (OR 0.217 (95%CI 0.052 to 0.901), p = 0.035). The results suggest that local PC cascade changes from a burn injury may be a separate process to the systemic effects and that the local levels may provide useful information in addition to the diagnostic and prognostic abilities we previously found in the circulating PC system.
Profitability and Firm Value: Does Dividend Policy Matter for Indonesian Sustainable and Responsible Investment (SRI)-KEHATI Listed Firms?
Authors: Akhmadi Akhmadi , Januarsi Yeni
Published: November 2021

This study investigates the potential factors explaining the inconsistent relationship between profitability and firm value. Specifically, it examines whether dividend policy contributes to the profitability–firm value relationship and determines the form of the moderating variables. We use a unique data set from the Indonesian capital market, with sustainable and responsible investment (SRI)-KEHATI-listed firms from 2010 to 2019. Adopting hierarchy moderating analysis, the results show a positive direct relationship between profitability and firm value. Moreover, the profitability–firm value relationship becomes stronger with a higher dividend policy ratio. We complement this with evidence that the dividend policy plays a role as a pure moderator in more sustainable and responsible firms. A sensitivity battery analysis and the endogeneity concern show consistent results as the baseline model, implying that the model is robust to different conditions. Additional tests revealed that the dividend policy is more prominent in low-leverage enterprises, low-intensity advertising firms, and during the financial service authority’s post-dividend policy regulation phase. The implication of our study is that corporate policy and country regulation play a role as a potential competitive strategy to increase shareholder value for SRI-KEHATI-listed firms.
Production and Maturation of Soaps with Non-Edible Fermented Olive Oil and Comparison with Classic Olive Oil Soaps
Authors: Ferracane Antonio , Tropea Alessia , Salafia Fabio
Published: November 2021

The study reports the alternative use of non-edible fermented olives for the production of high-quality natural soaps with a fast production process, low environmental impact, and without preliminary treatments for the raw material. Damaged olives, not used as food, were fermented naturally and the oil was extracted by mechanical extraction. The product obtained was not for human consumption due to its high acidity, but it had a low content of peroxides. The non-edible olive oil obtained and an extra virgin olive oil, produced from the same olive cultivar, were subjected to saponification with sodium hydroxide. The soaps were produced with complete (0% of non-neutralized fatty acids) and incomplete (5% of non-neutralized fatty acids) saponification; the amount of sodium hydroxide to be used was determined with the saponification index. The soaps were aged for six months by monitoring pH, color, and behavior in an aqueous solution. The results show that the olives’ fermentation improves and speeds up the soap production and maturation process since the oil obtained from fermented non-edible olives is more suitable for the saponification process than the oil obtained from non-fermented edible olives. Non-edible fermented olives can be used for obtaining natural and high-quality soaps, reusing drupes classified as food waste.
Sourdough Fermentation as a Tool to Improve the Nutritional and Health-Promoting Properties of Its Derived-Products
Authors: Graça Carla , Lima Ana , Raymundo Anabela , Sousa Isabel
Published: November 2021

Cereal products are staple foods highly appreciated and consumed worldwide. Nonetheless, due to the presence of gluten proteins, and other co-existing compounds such as amylase-trypsin inhibitors and fermentable short-chain carbohydrates in those products, their preference by consumers has substantially decreased. Gluten affects the small gut of people with celiac disease, triggering a gut inflammation condition via auto-immune response, causing a cascade of health disorders. Amylase-trypsin inhibitors and fermentable short-chain carbohydrate compounds that co-exists with gluten in the cereal-based foods matrix have been associated with several gastrointestinal symptoms in non-celiac gluten sensitivity. Since the symptoms are somewhat overlapped, the relation between celiac disease and irritable bowel syndrome has recently received marked interest by researchers. Sourdough fermentation is one of the oldest ways of bread leavening, by lactic acid bacteria and yeasts population, converting cereal flour into attractive, tastier, and more digestible end-products. Lactic acid bacteria acidification in situ is a key factor to activate several cereal enzymes as well as the synthesis of microbial active metabolites, to positively influence the nutritional/functional and health-promoting benefits of the derived products. This review aims to explore and highlight the potential of sourdough fermentation in the Food Science and Technology field.
Enhancing Efficiency of Anaerobic Digestion by Optimization of Mixing Regimes Using Helical Ribbon Impeller
Authors: Singh Buta , Kovács Kornél L. , Bagi Zoltán , Nyári József , Szepesi Gábor L. , Petrik Máté , Siménfalvi Zoltán , Szamosi Zoltán
Published: November 2021

The appropriate mixing system and approach to effective management can provide favorable conditions for the highly sensitive microbial community, which can ensure process stability and efficiency in an anaerobic digester. In this study, the effect of mixing intensity on biogas production in a lab-scale anaerobic digester has been investigated experimentally and via modeling. Considering high mixing efficiency and unique feature of producing axial flow, helical ribbon (HR) impeller is used for mixing the slurry in this experiment under various conditions. Three parallel digesters were analyzed under identical operating conditions for comparative study and high accuracy. Effects of different mixing speeds (10, 30, and 67 rpm for 5 min h−1) on biogas production rate were determined in 5-L lab-scale digesters. The results demonstrated 15–18% higher biogas production at higher mixing speed (67 rpm) as compared to 10 rpm and 30 rpm and the results proved statistically significant (p < 0.05). Biogas production at 10, 30, and 67 rpm were 45.6, 48.6, and 52.5 L, respectively. Higher VFA concentrations (7.67 g L−1) were recorded at lower mixing intensity but there was no significant difference in pH and ammonia at different speeds whereas the better mixing efficiency at higher speeds was also the main reason for increase in biogas production. Furthermore, model simulation calculations revealed the reduction of dead zones and better homogeneous mixing at higher mixing speeds. Reduction of dead zones from 18% at 10 rpm to 2% at 67 rpm was observed, which can be the major factor in significant difference in biogas production rates at various mixing intensities. Optimization of digester and impeller geometry should be a prime focus to scale-up digesters and to optimize mixing in full-scale digesters.
Anti-Oxidative and Anti-Inflammatory Activities of Astragalus membranaceus Fermented by Lactiplantibacillus plantarum on LPS-Induced RAW 264.7 Cells
Authors: Park Hye Min , Lee Ji Yeon , Kim Min Young , Kang Chang-Ho , Hwang Hyung Seo
Published: November 2021

Astragalus membranaceus (AM) has been used for anti-oxidative, anti-inflammatory, anti-cancer, and immunomodulatory activities. In this study, we confirmed that the anti-oxidative and anti-inflammatory effects of AM were enhanced after it was fermented by Lactiplantibacillus plantarum. The anti-oxidative effect was measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical levels, total phenolic contents (TPC), reducing power, and H2O2 levels. AM-LP MG5145 and MG5276 showed higher free radical scavenging activity than AM-NF (51.22%). In addition, AM-LP MG5145 and MG5276 showed higher TPC (49.11 mg GAE/mL), reducing power (OD700 = 0.37), and H2O2 (1.71 µM) than AM-NF. The calycosin contents determined to AM-NF (17.24 ng/mL), AM-LP MG5145 (139.94 ng/mL), and MG5276 (351.01 ng/mL) using UPLC-ESI-MS/MS. Anti-inflammatory effects were analyzed by investigating the inhibitory effects of fermented AM on cytotoxicity, NO production, and mRNA expression of COX-2, iNOS, NF-κB, and TNF-α in LPS-induced RAW 264.7 cells. AM-LP MG5145 and MG5276 showed no cytotoxicity. AM-LP MG5145 (50.86%) and MG5276 (51.66%) inhibited NO production in LPS-induced RAW 264.7 cells. Moreover, AM-LP MG5145 and MG5276 downregulated macrophage iNOS, COX2, TNF-α, and NF-κB expression. In conclusion, A. membranaceus fermented by L. plantarum MG5145 and MG5276 can be used in cosmetics and health foods as natural antioxidant compounds.
Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
Authors: Jawan Roslina , Abbasiliasi Sahar , Tan Joo Shun , Halim Murni , Mustafa Shuhaimi , Lee Bin Hao , Kwa Jia Sim , Ariff Arbakariya B.
Published: November 2021

This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cultivation and downstream processing of BLIS. Results showed that the molecular weight of PEG, PEG concentration, and dextran T500 affect the partition coefficient (K), purification factor (PF), and yield of BLIS partitioning. ATPS composed of 10% (w/w) PEG2000 and 8% (w/w) dextran T500, provided the greatest conditions for the extractive BLIS production. The K (1.00 ± 0.16), PF (2.92 ± 0.37) and yield (77.24 ± 2.81%) were increased at selected orbital speed (200 rpm) and pH (pH 7). Sustainable growth of the cells in the bioreactor and repeated fermentation up to the eighth extractive batch were observed during the scale up process, ensuring a continuous production and purification of BLIS. Hence, the simplicity and effectiveness of ATPS in the purification of BLIS were proven in this study.
Toward a Better Understanding of Sediment Dynamics as a Basis for Maintenance Dredging in Nagan Raya Port, Indonesia
Authors: Zikra Muhammad , Salsabila Shaskya , Sambodho Kriyo
Published: November 2021

The Port of 2 × 110 MW Nagan Raya Coal Fired Steam Power Plant is one of the facilities constructed by the State Electricity Company in Aceh Province, Indonesia. During its operation, which began in 2013, the port has dealt with large amounts of sedimentation within the port and ship entrances. The goal of this study is to mitigate the sedimentation problem in the Nagan Raya port by evaluating the effect of maintenance dredging. Field measurements, and hydrodynamic and sediment transport modeling analysis, were conducted during this study. Evaluation of the wind data showed that the dominant wind direction is from south to west. Based on the analysis of the wave data, the dominant wave direction is from the south to the west. Therefore, the wave-induced currents in the surf zone were from south to north. Based on the analysis of longshore sediment transport, the supply of sediments to Nagan Raya port was estimated to be around 40,000–60,000 m3 per year. Results from the sediment model showed that sedimentation of up to 1 m was captured in areas of the inlet channel of Nagan Raya port. The use of a passing system for sand is one of the sedimentation management solutions proposed in this study. The dredged sediment material around the navigation channel was dumped in a dumping area in the middle of the sea at a depth of 11 m, with a distance of 1.5 km from the shoreline. To obtain a greater maximum result, the material disposal distance should be dumped further away, at least at a depth of 20 m or a distance of 20 miles from the coastline.
Cocoa Nanoparticles to Improve the Physicochemical and Functional Properties of Whey Protein-Based Films to Extend the Shelf Life of Muffins
Authors: Calva-Estrada Sergio de Jesús , Jimenez-Fernandez Maribel , Vallejo-Cardona Alba Adriana , Castillo-Herrera Gustavo Adolfo , Lugo-Cervantes Eugenia del Carmen
Published: November 2021

A novel nanocomposite whey protein-based film with nanoemulsified cocoa liquor (CL) was prepared using one-stage microfluidization to evaluate the emulsion properties and the effect of CL on the film properties by response surface methodology (RSM). The results indicated that the number of cycles by microfluidization had a significant effect (p < 0.05) on the particle size and polydispersity of the nanoemulsion, with a polyphenol retention of approximately 83%. CL decreased the solubility (<21.87%) and water vapor permeability (WVP) (<1.57 g mm h−1 m−2 kPa−1) of the film. FTIR analysis indicated that CL modified the secondary protein structure of the whey protein and decreased the mechanical properties of the film. These results demonstrate that applying the film as a coating is feasible and effective to improve the shelf life of bakery products with a high moisture content. This nanocomposite film is easy to produce and has potential applications in the food industry.
Effect of Different Temperature-Controlled Ultrasound on the Physical and Functional Properties of Micellar Casein Concentrate
Authors: Song Bong , Zhang Yumeng , Yang Baojia , Zhu Panpan , Pang Xiaoyang , Xie Ning , Zhang Shuwen , Lv Jiaping
Published: November 2021

Micellar casein concentrate (MCC) is a novel dairy ingredient with high protein content. However, its poor functional properties impair its potential for further application, highlighting the importance of using innovative processing methods to produce modified MCC, such as ultrasound (US). This work investigated the impact of US on the physical and functional properties of MCC under temperature-controlled and -uncontrolled conditions for different time intervals. Under temperature-controlled ultrasound (TC-US) treatment, a reduction was found in the supernatant particle size of casein micelles. Soluble calcium content and hydrophobicity increased following ultrasound treatment at 20 °C, resulting in a remarkable improvement in emulsification. However, long-time ultrasonication led to an unstable state, causing the MCC solutions to show shear thinning behavior (pseudoplastic fluid). Compared with 50 °C temperature-controlled ultrasonication, ultrasonication at 20 °C had a greater influence on particle size, viscosity and hydrophobicity. These findings indicate that 20 °C TC-US could be a promising technology for the modification of MCC.
SlCCD1A Enhances the Aroma Quality of Tomato Fruits by Promoting the Synthesis of Carotenoid-Derived Volatiles
Authors: Cheng Guo-Ting , Li Yu-Shun , Qi Shi-Ming , Wang Jin , Zhao Pan , Lou Qian-Qi , Wang Yan-Feng , Zhang Xiang-Qian , Liang Yan
Published: November 2021

The loss of volatiles results in the deterioration of flavor in tomatoes. Volatiles are mainly derived from fatty acid, carotenoid, phenylpropane, and branched chain amino acids. In this study, the tomato accession CI1005 with a strong odor and accession TI4001 with a weak odor were analyzed. The volatile contents were measured in tomato fruits using gas chromatography-mass spectrometry. The scores of tomato taste and odor characteristics were evaluated according to hedonistic taste and olfaction. It was found that the content of fatty acid-derived volatiles accounted for more than half of the total volatiles that had grassy and fatty aromas. Phenylpropane-derived volatiles had irritation and floral aromas. Branched-chain amino acid-derived volatiles had a caramel aroma. Carotenoid-derived volatiles had floral, fruity, fatty, and sweet-like aromas, preferred by consumers. A lack of carotenoid-derived volatiles affected the flavor quality of tomato fruits. The accumulation of carotenoid-derived volatiles is regulated by carotenoid cleavage oxygenases (CCDs). A tissue-specific expression analysis of the SlCCD genes revealed that the expression levels of SlCCD1A and SlCCD1B were higher in tomato fruits than in other tissues. The expression levels of SlCCD1A and SlCCD1B were consistent with the trend of the carotenoid-derived volatile contents. The expression of SlCCD1A was higher than that for SlCCD1B. A bioinformatics analysis revealed that SlCCD1A was more closely linked to carotenoid metabolism than SlCCD1B. The overexpression of SlCCD1A indicated that it could cleave lycopene, α-carotene, and β-carotene to produce 6-methyl-5-hepten-2-one, geranylacetone, α-ionone, and β-ionone, increasing the floral, fruity, fatty, and sweet-like aromas of tomato fruits. The flavor quality of tomato fruits could be improved by overexpressing SlCCD1A.
Tricholoma matsutake-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
Authors: Li Mengqi , Ge Qi , Du Hanting , Lin Songyi
Published: November 2021

Tricholoma matsutake is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of T. matsutake. Our aim was to evaluate the protective effects and molecular mechanisms of two T. matsutake-derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. Tricholoma matsutake peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of T. matsutake peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with T. matsutake peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that T. matsutake peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage.
Implication of Row Orientation Changes on Fruit Parameters of Vitis vinifera L. cv. Riesling in Steep Slope Vineyards
Authors: Strack Timo , Stoll Manfred
Published: November 2021

Row orientation, among others, is a crucial factor in determining grapevine performance and health status, thus affecting berry components that form the basis of the later wine profile. However, the literature about the impact of changes in row orientation at steep slope sites on grapevine fruit composition as well as the differentiation between canopy sides hardly exists. Thus, the aim of this work was to gain knowledge about the impact of row orientation in steep slope vineyards on selected primary and secondary metabolites in berries of Vitis vinifera L. cv. Riesling. Samples were taken from both canopy sides of different row orientations of terraced and downslope vineyards in steep slopes. Free amino acids in the juice and flavonols in the berry skin had a positive correlation to sunlight exposure. Furthermore, grapevines showed adaptations to constantly higher light conditions, e.g., physiologically in reduction in chlorophyll content or protective mechanisms resulting in a lower susceptibility to sunburn damage. Thus, grapevine fruit parameters are affected by row orientation change in steep slopes.
Probiotics and Isoflavones as a Promising Therapeutic for Calcium Status and Bone Health: A Narrative Review
Authors: Harahap Iskandar Azmy , Suliburska Joanna
Published: November 2021

Probiotics have potential clinical effects for treating and preventing osteoporosis. Meanwhile, isoflavones have attracted much attention due to their ability to prevent postmenopausal symptoms. Research has established that probiotics and isoflavones can regulate hormones, immune cells, and the gastrointestinal system, acting as links in the gut–bone axis. However, combining the effects of probiotics and isoflavones on calcium status and bone health is a more novel and a still-evolving research area. Lactobacillus and Bifidobacterium are the foremost strains that influence bone health to a significant extent. Among the isoflavones, daidzein, genistein, and the metabolites of genistein (such as equol) stimulate bone formation. It can be concluded that probiotics and isoflavones promote bone health by regulating calcium uptake, gut microbiota, and various metabolic pathways that are associated with osteoblast activity and bone formation. Nevertheless, further experiments of probiotics and isoflavones are still necessary to confirm the association between calcium bioavailability and bone health.
Astragalus Polysaccharides and Saponins Alleviate Liver Injury and Regulate Gut Microbiota in Alcohol Liver Disease Mice
Authors: Zhou Jingxuan , Zhang Nanhai , Zhao Liang , Wu Wei , Zhang Liebing , Zhou Feng , Li Jingming
Published: November 2021

Astragalus, a medical and edible plant in China, shows several bioactive properties. However, the role of astragalus in attenuating alcoholic liver disease (ALD) is less clear. The objective of this project is to investigate the improving effect of astragalus saponins (AS) and astragalus polysaccharides (AP), which are the two primary constituents in astragalus on hepatic injury induced by alcohol, and the potential mechanisms of action. Different doses of AS (50 and 100 mg/kg bw) and AP (300 and 600 mg/kg bw) were orally given to alcohol-treated mice for four weeks. The results demonstrated that both AP and AS could reverse the increase of the levels of TC, TG, FFA, and LDL-C in serum, and the decrease of serum HDL-C content, as well as the elevation of hepatic TC and TG levels induced by alcohol. The activities of AST, ALT, ALP, and γ-GT in ALD mice were raised after AP and AS supplementation. The antioxidant markers (SOD, CAT, GSH, and GSH-Px) were obviously augmented and the pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) and hepatic histological variations were alleviated by AP and AS, which was in line with the levels of oxidative stress-associated genes (Keap1, Nfe2l2, Nqo1, and Hmox1) and inflammation-associated genes (Tlr4, Myd88 and Nfkb1). In addition, AS exerted a more efficient effect than AP and the results presented dose proportionality. Moreover, AS and AP could modulate the intestinal microbiota disturbance induced by alcohol. Overall, AS and AP administration could ameliorate lipid accumulation in the serum and liver, as well as hepatic function, oxidative stress, inflammatory response, and gut flora disorders in mice as a result of alcohol.
Searching for High-z Radio Galaxies with the MGCLS
Authors: Knowles Kenda , Manaka Sinah , Bietenholz Michael F. , Cotton William D. , Hilton Matthew , Kolokythas Konstantinos , Loubser S. Ilani , Oozeer Nadeem
Published: November 2021

We present the results from a search for high-redshift radio galaxy (HzRG) candidates using 1.28 GHz data in the Abell 2751 field drawn from the MeerKAT Galaxy Cluster Legacy Survey (MGCLS). We used the HzRG criteria that a radio source is undetected in all-sky optical and infrared catalogues and that it has a very steep radio spectrum. We used the likelihood ratio method for cross-matching the radio catalogue against multi-wavelength galaxy catalogues from the Dark Energy Camera Legacy Survey (DECaLS) and the All-sky Wide Infrared Survey Explorer (AllWISE). For those radio sources with no multi-wavelength counterpart, we further implemented a radio spectral index criterium of α<−1, using in-band spectral index measurements from the wide-band MeerKAT data. Using a 5σ signal-to-noise cut on the radio flux densities, we found a total of 274 HzRG candidates: 179 ultra-steep spectrum sources and 95 potential candidates, which could not be ruled out as they had no spectral information available. The spectral index assignments in this work were complete above a flux density of 0.3 mJy, which is at least an order of magnitude lower than existing studies in this frequency range or when extrapolating from lower frequency limits. Our faintest HzRG candidates with and without an in-band spectral index measurement had a 1.28 GHz flux density of 57 ± 8 μJy and 68 ± 13 μJy, respectively. Although our study is not complete down to these flux densities, our results indicate that the sensitivity and bandwidth of the MGCLS data make them a powerful radio resource to search for HzRG candidates in the Southern sky, with 20 of the MGCLS pointings having similar image quality as the Abell 2751 field and full coverage in both DECaLS and AllWISE. Data at additional radio frequencies will be needed for the faintest source populations, which could be provided in the near future by the MeerKAT UHF band (580–1015 MHz) at a similar resolution (∼8–10″).
Synthesis and Characterization of ZnO(MgO)-CaO-SiO2-P2O5 Bioglass Obtained by Sol-Gel Method in Presence of Surfactant Agent
Authors: Ghiţulică Cristina-Daniela , Damian-Buda Andrada-Ioana , Cucuruz Andreia , Voicu Georgeta
Published: November 2021

Bioglass (BG) is a class of biomaterials increasingly approached in biomedical applications, such as in regeneration of hard tissues, due to the properties of bioactivity, osteoinductivity, osteoconductivity, but also the high rate of biodegradation, both in vitro and in vivo. The present paper addresses the obtaining of bioglasses from the ZnO(MgO)-CaO-SiO2-P2O5 system by the sol-gel method and the use of a surfactant to ensure a specific surface or high open porosity, starting from S53P4 bioglass (53% SiO2, 23% Na2O, 20% CaO, 4% P2O5), also known as BoneAlive®. The precursor powders were analyzed from the phase composition point of view by complex thermal analysis and X-ray diffraction, the vitreous powders were assessed from the compositional point of view by X-ray diffraction, morpho-structural by scanning electron microscopy, specific surface area and the pore size dimension by the Brunauer–Emmett–Teller (BET) analysis, dispersion by laser granulometry, and also cell biology and surface mineralization tests were performed by immersion in SBF (simulated body fluid). The system proposed in this paper ZnO(MgO)-CaO-SiO2-P2O5 was successfully obtained by sol-gel method. The results showed the higher interaction between the samples and the SBF medium for samples containing magnesium (M2) and the lowest degree of mineralization after immersion in SBF was noticed for samples containing zinc (M1). The results also prove that by incorporating different ionic species in bioglass composition—Zn2+ and Mg2+, biocompatibility and antibacterial properties will be significantly enhanced.
Kinetics of the Release of Nicotinamide Absorbed on Partially Neutralized Poly(acrylic-co-methacrylic acid) Xerogel under the Conditions of Simultaneous Microwave Heating and Cooling
Authors: Jovanovic Jelena D. , Adnadjevic Borivoj K.
Published: November 2021

The kinetics of release of nicotinamide (NIAM) that was absorbed on partially neutralized poly(acrylic-co-methacrylic) (PAM) xerogel/hydrogel, under the conditions of simultaneous microwave heating and cooling (SMHC) were examined. The kinetics curves of NIAM release into an aqueous solution at temperatures of 308–323 K were recorded. By applying the model-fitting method (MFM), it was found that the kinetics of NIAM release can be modeled by a kinetic model of a first-order chemical reaction. The values of the release rate constants (kM) at different temperatures were calculated, and their values were found to be within the range 8.4 10−3 s −1−15.7 10−3 s−1. It has been established that the Arrhenius equation was valid even in the conditions of SMHC. The values of the kinetic parameters (activation energy (Ea) and pre-exponential factor (A) of the NIAM release process adsorbed on PAM xerogel/hydrogel were calculated as follows: Ea = 25.6 kJ/mol and ln (A/s−1) = 5.21. It has been proven that the higher value of the rate constant at SMHC in relation to CH is not a consequence of the overheating of the reaction system or the appearance of “hot-points”. The values of change of the enthalpy of activation (ΔH*) and the change of entropy of activation (ΔS*) were calculated as follows: ΔH* = +23.82 kJ/mol and ΔS* = −201.4 J/mol K. The calculated higher values of the kinetic parameters and thermodynamic parameters of activation are explained by the formation of a specific activated complex under SMHC, whose structure and degree of order are different than in the one formed under CH.
Factors Affecting the Quality of Life of Patients with Painful Spinal Bone Metastases
Authors: Akezaki Yoshiteru , Nakata Eiji , Kikuuchi Masato , Sugihara Shinsuke , Katayama Yoshimi , Katayama Haruki , Hamada Masanori , Ozaki Toshifumi
Published: November 2021

This study examined changes in the quality of life (QOL), as well as the factors affecting QOL, among patients with painful spinal bone metastases without paralysis for 1 month after radiotherapy. Methods: This study included 79 participants (40 male and 39 female; median age, 65 (42–88) years) who had undergone radiotherapy for painful spinal bone metastases without paralysis. Patients’ age, sex, activities of daily living (Barthel index), pain, spinal instability (spinal instability neoplastic score [SINS]), and QOL (EORTC QLQ-C30) were investigated. Results: Having an unstable SINS score was a positive factor for global health status (p < 0.05). The improvement in activities of daily living and response to pain were positive factors for physical function (p < 0.05). A positive effect on emotional function was confirmed among female patients (p < 0.05). Conclusion: Engaging in rehabilitation along with radiotherapy leads to improvements in QOL for patients with spinal bone metastases.
Genotoxicity and Cytotoxicity Induced in Zygophyllum fabago by Low Pb Doses Depends on the Population’s Redox Plasticity
Authors: López-Orenes Antonio , Santos Conceição , Dias Maria Celeste , Oliveira Helena , Ferrer María Á. , Calderón Antonio A. , Silva Sónia
Published: November 2021

Lead (Pb) soil contamination remains a major ecological challenge. Zygophyllum fabago is a candidate for the Pb phytostabilisation of mining tailings; nevertheless, the cytogenotoxic effects of low doses of Pb on this species are still unknown. Therefore, Z. fabago seeds collected from non-mining (NM) and mining (M) areas were exposed to 0, 5 and 20 µM Pb for four weeks, after which seedling growth, Pb cytogenotoxic effects and redox status were analyzed. The data revealed that Pb did not affect seedling growth in M populations, in contrast to the NM population. Cell cycle progression delay/arrest was detected in both NM and M seedlings, mostly in the roots. DNA damage (DNAd) was induced by Pb, particularly in NM seedlings. In contrast, M populations, which showed a higher Pb content, exhibited lower levels of DNAd and protein oxidation, together with higher levels of antioxidants. Upon Pb exposure, reduced glutathione (GSH) and non-protein thiols were upregulated in shoots and were unaffected/decreased in roots from the NM population, whereas M populations maintained higher levels of flavanols and hydroxycinnamic acids in shoots and triggered GSH in roots and shoots. These differential organ-specific mechanisms seem to be a competitive strategy that allows M populations to overcome Pb toxicity, contrarily to NM, thus stressing the importance of seed provenance in phytostabilisation programs.
Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
Authors: Viejobueno Josefina , Rodríguez-Berbel Natalia , Miranda Luis , de los Santos Berta , Camacho María
Published: November 2021

The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseolina (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against M. phaseolina in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to Brevibacterium genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to M. phaseolina decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that Brevibacterium sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry.