bluuebunny/crossref_metadata_2025 huggingface dataset:
Screening, identification and characterisation of Polyhydroxybutyrate producing bacteria from garden soil
Authors: Mahitha S , Premila J M Sasi , Chelliah D Abiya
Published: November 2022

Plastic pollution need to be resolved as it affects air, water, land. The favourable alternative for plastics would be Polyhydroxybutyrate (PHB) from bacterial origin, which are biodegradable and biocompatible biopolymers. Focus on the PHB producing bacteria is done by collecting garden soil sample. Five colonies of Sudan black blue positive isolates were chosen, extracted, and produced. One of the strains (SM1) - a potent producer as confirmed by crotonic acid assay, was further subjected to large scale production. The PHB thus produced was analysed using Fourier Transform Infra-Red spectroscopy (FTIR) and Gas Chromatography Mass Spectrometry (GCMS) to confirm the presence of functional groups. X-Ray crystallography revealed that it is of crystalline nature and are pictured by Scanning electron microscopy photography. DNA was isolated from the strain SM1, and the gene for 16S rRNA has been sequenced and submitted in GENBANK, (Accession No: MZ363886). The organism was found to be Bacillus cereus as predicted by 16S rRNA and NCBI BLAST. A phylogenetic tree was constructed using MEGA software. Bioplastic preparation was done under laboratory scale and the produced bioplastic was successfully degraded using Pseudomonas species. The prepared bioplastic from bacteria was biodegradable and eco-friendly.
A Fast Retrieval Algorithm for Person Re-identification Based on Hash
Authors: Huang Yewen , Xu Zhicong , Shan Chun
Published: November 2022

Aiming at the problem of slow retrieval speed due to a large amount of data in person re-identification, this paper introduces Taylor expansion into a deep neural network, and obtains Taylor deep neural network for feature extraction, which is combined with the proposed hash probability coding algorithm, and get a fast retrieval algorithm for person re-identification based on the hash. The algorithm can not only extract the feature map information well but also perform image retrieval at a faster speed, which is a new method to solve the fast image retrieval of person re-identification. First, a Taylor deep neural network framework is proposed, and the feature representation extracted by each layer of the deep network is fused by Taylor expansion weighting to retain the overall features of the image and make the feature representation more comprehensive ; then, the triplet loss function Triplet Loss is optimized to improve the When the distance between groups cannot be optimized, the group loss function Group Loss is proposed. Finally, a new hash probability compression coding method is used to make the image retrieval fast. Experiments show that the algorithm model in this paper is 26.7% and 8.18% higher than the BaseLine model on the large data sets Cuhk03 and Market1501, respectively. It also has a good retrieval speed on images retrieved by person re-identification..
Visceral localizations and mortality in catheter-associated fungemia
Authors: MERIGLIER Etienne , VANDENHENDE Marie-Anne , BESSEDE Emilie , RIVOISY Claire , CHAUSSADE Helene , BRONNIMANN Didier , BONNET Fabrice
Published: November 2022

Purpose: To describe the prevalence of visceral localizations, risk factors and overall mortality at 3 months in catheter-associated fungemia in ICUs and non-ICUs
Method: Retrospective study of patients with a fungal organism isolated on catheter blood culture. Clinical characteristics, visceral localizations, management and outcome were examined.
Results: One hundred forty five patients presented primary or secondary fungemia (median age 61 years, 57% males). Sixty visceral localizations occurred in 50 patients (34.5%) including digestive (n= 21), pulmonary (n=11), vascular (n=6), spleen (n=4); muscle (n=3); cerebral (n=2); liver (n=2); ocular (n=2), hip prosthetic joint infection (n=1), spondylodiscitis (n=1) and abdominal wall (n=1). There was no statistical association between visceral localizations and fungal agent (p=0.208) or type of catheters (p=0.225). Fifty-two patients (36%) died during follow-up. Overall mortality was associated with retention of the central line catheter (p<0.01), type of catheter (p=0.004), Charlson Comorbidity Index (p< 0.01), inadequate or absence of antifungal therapy (p<0.01), hospitalization in an intensive care unit (p<0.01) and renal failure (p=0.02).
Conclusion: Visceral localizations are common and not significantly associated with mortality in catheter-associated fungemia. Visceral localizations were not associated with the type of fungal agent and the type of catheter. Overall mortality is important and associated with important predisposing host conditions, the retention of the central line catheter and the type of catheter.
Preservation of KCC2 expression in axotomized abducens motoneurons and its enhancement by VEGF
Authors: Calvo Paula M. , Cruz Rosa R. de la , Pastor Angel M. , Alvarez Francisco J.
Published: November 2022

The potassium chloride cotransporter 2 (KCC2) is the main Cl− extruder in neurons. Any alteration in KCC2 levels leads to changes in Cl− homeostasis and, consequently, in the sign and amplitude of inhibitory synaptic potentials mediated by GABA or glycine. Axotomy downregulates KCC2 in many different motoneurons and it is suspected that interruption of muscle-derived factors maintaining motoneuron KCC2 expression is in part responsible. In here, we demonstrate that KCC2 is expressed in all oculomotor nuclei of cat and rat, but while trochlear and oculomotor motoneurons downregulate KCC2 after axotomy, expression is unaltered in abducens motoneurons. Exogenous application of vascular endothelial growth factor (VEGF), a neurotrophic factor expressed in muscle, upregulated KCC2 in axotomized abducens motoneurons above control levels. In parallel, a physiological study using cats chronically implanted with electrodes for recording abducens motoneurons in awake animals, demonstrated that inhibitory inputs related to off-fixations and off-directed saccades in VEGF-treated axotomized abducens motoneurons were significantly higher than in control, but eye-related excitatory signals in the on direction were unchanged. This is the first report of lack of KCC2 regulation in a motoneuron type after injury, proposing a role for VEGF in KCC2 regulation and demonstrating the link between KCC2 and synaptic inhibition in awake, behaving animals.
Treatment of Embroidery Wastewater Containing Poly (Vinyl Alcohol) by Electrooxidation Process
Authors: Gengec Erhan , Bozbas Seda Karayunlu , Ayhan Hasan Huseyin
Published: November 2022

Due to its high poly(vinyl) alcohol (PVA) concentration, Embroidery industry wastewater is very resistant to biodegradation and classical methods such as coagulation, and adsorption. On the other hand, high chemical consumption is required in chemical oxidation processes. In this study, wastewater containing PVA was treated by an electrooxidation process with Boron Doped Diamond (BDD) electrodes for the first time in literature. The effects of pH (2.0–8.0), temperature (25–45 ̊C), current (3.0–6.0 A), electrolyte concentration (NaCl of 3.0–8.0 g/L), and oxidation time (0-600 min) were determined. The total organic carbon (TOC) concentration in the wastewater was decreased from 6584 mg/L to 159.10 mg/L (97.6%) at pH 5.01, 6.0 A, 3.0 g/L of electrolyte concentration, 25°C, and 10 hours. In addition, the removal mechanism of the process was elucidated by using HPSEC, FT-IR, UV-VIS, and Fluorescence Spectroscopy methods. Characterization studies showed that the high amount of PVA in the embroidery process wastewater is degraded and the halogens have attached to the part of the polymer where the structure has deteriorated through oxidation. Raw wastewater contains a wide range of pollutants (103-1010 Da). The results showed that the EO process is not a pollutant-selective technique in terms of molecular weight and it shows its superiority over other processes.
FGFR2 controls growth, adhesion and migration of nontumorigenic human mammary epithelial cells by regulation of integrin β1 degradation
Authors: Mieczkowski Kamil , Popeda Marta , Lesniak Dagmara , Sadej Rafal , Kitowska Kamila
Published: November 2022

The role of fibroblast growth factor receptor 2 (FGFR2), an important mediator of stromal paracrine and autocrine signals, in mammary gland morphogenesis and breast cancer has been extensively studied over the last years. However, the function of FGFR2 signalling in the initiation of mammary epithelial oncogenic transformation remains elusive. Here, FGFR2-dependent behaviour of nontumorigenic model of mammary epithelial cells was studied. In vitro analyses demonstrated that FGFR2 regulates epithelial cell communication with extracellular matrix (ECM) proteins. Silencing of FGFR2 significantly changed the phenotype of cell colonies in three-dimensional cultures, decreased integrins α2, α5 and β1 protein levels and affected integrin-driven processes, such as cell adhesion and migration. More detailed analysis revealed the FGFR2 knock-down-induced proteasomal degradation of integrin β1. Analysis of RNA-seq databases showed significantly decreased FGFR2 and ITGB1 mRNA levels in breast tumour samples, when compared to non-transformed tissues. Additionally, high risk healthy individuals were found to have disrupted correlation profiles of genes associated with FGFR2 and integrin signalling, cell adhesion/migration and ECM remodelling. Taken together, our results strongly suggest that FGFR2 loss with concomitant integrin β1 degradation is responsible for deregulation of epithelial cell-ECM interactions and this process may play an important role in the initiation of mammary gland epithelial tumorigenesis.
MethMotif.org 2023: The transcription factor binding motifs database that integrates cell-specific functions, co-factors and DNA methylation profiles.
Authors: Dyer Matthew , Lin Quy Xiao Xuan , Shapoval Sofiia , Thieffry Denis , Benoukraf Touati
Published: November 2022

Motivation: Transcription factors binding events depend not only on a specific DNA motif but also on the chromatin context. To further explore this new transcriptional machinery level of complexity, we developed TFregulomeR, a MethMotif API R-library that facilitates batch queries. Our toolbox showed how the functions of TFs depend on cofactors, whose recruitment are also influenced by DNA methylation. This observation led us to release a new version of MethMotif that includes, in addition to more methyl-TF motifs and more organisms (Mus Musculus and Arabidopsis Thaliana), new features to discern context-specific TFs’ motifs associated with context-specific TFs' functions.
Results: Using TFregulomeR, we expanded the range of information available in the new release of MethMotif by segregating TF’s binding motifs in the context of their cofactors and DNA methylation status. This strategy enabled us to build the first TF binding motifs database that incorporates context-specific position weight matrices coupled to epigenetic information and context-specific TFs’ function. Using this repository, we were able to further demonstrate that gene ontology (GO) enriched terms associated with transcription factor target genes may differ notably based on association with alternative cofactors and DNA methylation status.
Conclusions: Overall, this update turns MethMotif into an integrative TF motif database with diverse regulatory element analysis tools accessible to a broad audience. Ultimately, this repository and its companion tools greatly facilitate the analysis of the consequences of epigenetic aberrations seen in diseases such as cancers.
Availability: The MethMotif database is freely available at https://methmotif.org.
Study of heat transfer under the influences ofpressure gradient and thermal non-equilibrium onDarcy-Bénard convection
Authors: N Sakshath T , V Chandan B
Published: November 2022

The study deals with the effect of pressure gradient on the onset of Darcy-Bénard convection of water-copper nanoliquidsaturatedporous layer and on amount of heat transport. The present study assumes thermal non-equilibrium (LTNE) betweensolid and liquid phases. The LTNE assumption results in advanced onset of convection and increase in heat transportwhen compared to that of local thermal equilibrium (LTE) assumption. Free-Free, isothermal boundaries are consideredfor investigation. Critical Rayleigh number and Nusselt number as functions of various parameters are analysed and this isdepicted graphically. The influence of pressure gradient, inter-phase heat transfer coefficient, ratio of thermal conductivities onthe onset of convection as well as on heat transport has been presented graphically and discussed in detail. The effect ofincreasing the value of porosity modified thermal conductivities advances the onset of convection and enhances the amount ofheat transport whereas the remaining parameters have an opposing influence on both onset of convection as well as heattransport. The classical results of LTE case are obtained as a limiting case of LTNE case for small values of H and large valuesof H, γ and γH.
Do trees on agrarian stone walls respond to contemporary climate warming?
Authors: Shetti Rohan , Lehejček Jiří , Zacharová Johana
Published: November 2022

Agrarian stone walls and associated landscapes are present in different parts of Central and Western Europe. Somewhere they are conserved as valuable components of cultural landscapes whereas elsewhere they do not have any formal conservation status. These stone walls and the habitats connected to them host many old trees, which are potential archives of environmental changes. In the available literature there are practically no studies looking at the archival value of trees on agrarian stone walls. In our study we investigated sycamore maples growing on stone walls in the Ore Mts region of Czechia. We focussed on annual wood growth using dendrochronological methods and carried out pointer year analyses to examine environment-growth relationships. Our main findings suggest moderately heterogeneous growth among individuals within sites but that there is a common environmental response apparent in overall site chronologies. We observed low inter-series correlations within sites but only a fair to moderate Gleichläufigkeit and express population signal among ring width series at most of the study sites. Generally, we revealed mostly significant positive correlations with precipitation and negative correlations with increasing temperatures over the last years. From climate correlations analysis and pointer year analysis we could clearly infer that (1) tree growth on agrarian stone walls at three out of four of our sites is significantly impacted by current climate change and that (2) these trees have a good ecological-archival value because they are sensitive to contemporary warming; they should therefore be identified to be given a relevant conservation status and management.
Differential alterations in topology property of resting state networks associated with mental health and childhood trauma
Authors: Wu Yankai , Fu Kailiang , Feng Xuran , Wang Yuzhao , Li Ling , Gao Duo , Geng Zuojun
Published: November 2022

Background
Childhood trauma experiences leads to greater psychopathological vulnerability. We identified potential brain networks that are susceptible to childhood trauma, and to investigate these changes mediate childhood adversity and mental health.
Methods
In total 80 youth aged 18–30 years participants, subjects with and without childhood trauma underwent resting-state functional magnetic resonance imaging scanning. Independent component analysis (ICA) and graph theory based on resting-state functional networks were compared between the two groups. We established a correlation analysis between changes in topological properties and neglect/abuse, and explored the mediating effect on childhood trauma and mental health. In addition, sex as a moderator of relationships between neglect and change in the global efficiency within-network connectivity was confirmed.
Results
Our findings suggest that childhood trauma is associated with altered global efficiency of salience network (SAN) and default mode network (DMN). Compared with male, neglect was more likely to change global efficiency of the SAN in female. There were significant roles of changes in global efficiency of SAN/DMN as mediators between neglect and novelty-seeking of three-dimensional personality (TPQ-NS).
Conclusions
These findings highlight childhood trauma experiences altered resting-state functional networks in healthy youth. Such abnormality of brain circuitry is relevant to DMN and SAN especially.
Will the circle be unbroken? The climate mitigation and sustainable development given by a circular economy of carbon, nitrogen, phosphorus and water
Authors: McKenna Patrick , Banwart Steven , Evans Barbara , Zakaria Fiona , Guest Jeremy
Published: November 2022

Closing the loop in the flow of C, nutrients and water between agriculture, the human diet and sanitation services offers benefits for humanity across multiple platforms of public health, food security and climate mitigation. This study assesses these benefits by describing the hypothetical scenario of a global, ‘fully functional’ circular economy, in which 100% of C, N and P were recovered from human excreta and returned to agricultural soil. Crop nutrient demand is calculated and compared with that which could be recovered, and greenhouse (GHG) emissions from fertilizer production, fertilizer application and sanitation services are presented, as are freshwater availability and crop irrigation requirements. These are considered to analyse the broader effects of this circular economy that is driven by dietary nutrition demand on climate change, the provision of sanitation services and crop irrigation, in 2022 and with projections to 2030 and 2050. We find the capacity of the circular economy to deliver crop nutrients and mitigate GHG emissions varies by region. Some regions benefit from supplementing conventional mineral fertilizers with excreta-derived fertilizers, others from reducing GHG emissions from sanitation services through improved resource recovery rates. A hypothetical, fully functional circular economy that recovers all excreta nutrient C, N and P would reduce global GHG emissions from N and P mineral fertilizer production and application by 140 Tg CO2 equivalents (CO2e) year-1 in 2022 (~12% of total emissions from mineral fertilizer production and application) and provide a maximum of 104 Tg C y-1 for sequestration in global cropland (~12% of estimated annual soil C sequestration potential). A portion of this sequestered C will return to the atmosphere via soil respiration, however, with co-benefits to other soil functions such as crop nutrient fertility. The maximum potential reduction in GHG emissions from sanitation services through these measures would bring reductions of 445 Tg CO2e year-1 in 2022, rising to 562 Tg CO2e in 2050. Our results provide evidence to guide specific regional policy on reducing GHG emissions, offsetting mineral fertilizer use and optimizing municipal water use using the circular economy. Our global results illustrate how the circular economy can make a significant contribution to achieving the Paris Accord both currently and going forward to 2050.
FINCA syndrome beyond pulmonary affection: biallelic NHLRC2 variants in eight families with intellectual disability and epilepsy
Authors: Boschann Felix , Sczakiel Henrike , Zhao Max , Danyel Magdalena , Stoltenburg Corinna , Damseh Nadirah , Ashhab Motee , Balci Tugce , Engelen Kalene van , Osmond Matt , Schallner Jens , Porrmann Joseph , McDonald Kimberly , Liao Mingjuan , Oppermann Henry , Platzer Konrad , Dierksen Nadine , Mojarad Majid , Eslahi Atieh , Bakaeean Behnaz , Maroofian Reza , Ehmke Nadja , Seelow Dominik , Horn Denise
Published: November 2022

FINCA syndrome is an autosomal recessive inherited multisystemic disorder characterized by pulmonary fibrosis, neurodegeneration and cerebral angiomatosis. So far, 13 patients from nine families with biallelic NHLRC2 variants have been published. In all of them, the recurrent missense variant p.(Asp148Tyr) was detected at least on one allele. Common manifestations comprised pulmonary fibrosis, respiratory distress, developmental delay, muscular hypotonia, dystonia, seizures and brain atrophy, followed mostly by early demise due to progression of disease. Here, we present ten individuals from eight families with an overlapping but static phenotype with much longer survival, associated with seven novel NHLRC2 variants identified by exome analysis. All of the here described patients presented with severe global developmental delay. While seizures and EEG abnormalities were observed as frequent manifestations, eight individuals did not show any signs of pulmonary involvement or distinct MRI abnormalities. Notably, we also present the first seven cases in which the recurrent p.(Asp148Tyr) variant was not detected, neither in homozygous nor in compound heterozygous state. Interestingly, none of these cases presented with the classic FINCA phenotype. However, bioinformatic modeling and analyses could not establish a distinct genotype phenotype correlation. Taken together, our findings broaden the known phenotypic and molecular spectrum and propose that NHLRC2 related disease should also be considered in patients presenting with intellectual disability, movement disorders, neuroregression and epilepsy without pulmonary findings.
Assessment of Air Pollution Tolerance Index (APTI) and Anticipated Performance Index (API) of selected Roadside plant species for the Green Belt Development at Ratnagiri City in the Konkan Region of Maharashtra, India
Authors: Patil Pandurang , Goud Ajay , Patil Pratiraj , Jadhav Kailas
Published: November 2022

Small towns are becoming hotspots of pollution due to industrial, urbanisation, and domestic activities. Air pollution affects human health and it is also, responsible for physiological changes in plants. Green belt development programmes are cost-effective for the minimisation of air pollution. In the present study, to calculate air pollution tolerance index (APTI) and anticipated performance index (API), samples of 25 plant species were collected from each area i.e. the Industrial (I), Urban (U), and Rural (R) areas and analysed for different parameters. Amongst all three areas, APTI of Artocarpus-heterophyllus (46.74), Calotropis gigantea (43.63), and Bauhinia racemose (42.11) have shown the highest values and these plants can act as an inhibitor of air pollution. Also, the APTI of Ocimum tenuiflorum has found to be the lowest (12.05, 11.32, 12.86) as compared to other plant species among the three areas. Statistical analysis reveals that values of R2 are consistent in case of Total Chlorophyll (TC) and Ascorbic Acid (AA). API index showed the efficiency of Calotropis gigantea (excellent), Artocarpus-heterophyllus (very good), and Mangifera Indica (very good) for the green belt development around the selected areas. It is recommended to plant above mentioned plant species along the roadside by considering their air pollution tolerance ability and medicinal as well as economic importance. Further, it is suggested to plant species of Artocarpus-heterophyllus (Jackfruit) and Mangifera Indica (Alphonso Mango) which will generate income source for the local government bodies (Ratnagiri Municipal Council), as the fruits and wood of these plants can be exported and sold.
Viral shedding among symptomatic COVID-19 cases infected with the ancestral strain and Omicron BA.2
Authors: Wu Peng , Lin Yun , Tsang Tim , Wong Jessica , Lau Eric H. Y. , Yang Bingyi , Leung Gabriel , Cowling Benjamin
Published: November 2022

Assessment of viral kinetics of SARS-CoV-2 may inform host immune responses to and transmission potential of the virus. We analyzed longitudinal data of RT-PCR tests conducted for symptomatic COVID-19 patients at confirmation and during hospital isolation in Hong Kong during three major epidemic waves, and modeled the temporal trajectories of viral burden in these patients. Our analysis demonstrated that a longer duration of viral shedding appeared in more severe and older cases. Vaccinated individuals with a breakthrough infection of Omicron BA.2 exhibited a lower viral burden than non-vaccinated Omicron cases in early days following onset and a shorter duration of viral shedding particularly in more recently vaccinated patients. The viral kinetics characterized in our study provided insight into further studies on host immune responses to SARS-CoV-2 infection, prognosis of COVID-19 patients as well as possible protective mechanisms of vaccination against infection and severe outcomes.
Low-Computation GNSS Acquisition Method for Sparse Doppler Frequency Hypotheses in High-dynamic Environment
Authors: wu chao
Published: November 2022

To acquire Global Navigation Satellite System (GNSS) signal detection means bit transition, Doppler frequency, Doppler rate and code phase four-dimension acquisition in high-dynamic and long integration applications, and this detection needs a high computational cost. To reduce the computations of parameters estimation, this paper proposes a low-computation GNSS acquisition method (LGAM) in the high-dynamic environment. Firstly, sparse Doppler frequency (SDF) process is performed for SDF hypotheses, and post-correlation signal model is derived based on SDF structure. Then, double-FFT based detection is proposed based on the post-correlation signal model for parameters estimation. The results demonstrate that due to the reduction of complex multiplications, the computational cost of LGAM is lower than that of the FFT based methods under the moderate signal to noise ratio (SNR).
Pineapple Field Establishment Using Slips
Authors: Paull Robert E. , Wiseman Benjamin , Uruu Gail
Published: November 2022

Slips are side-shoots or fruit with large crowns that grow from buds on the pineapple (Ananas comosus L.) peduncle. The slips are widely used for pineapple vegetative propagation when crowns are left attached to the fruit that is marketed. There is a difference between the two most popular low-acid pineapple hybrids grown worldwide. The ‘Pineapple Research Institute 73-50’ (CO-2, MD-1) slips develop few roots when planted compared with ‘Pineapple Research Institute 73-114’ (MD2). The slow rooting of 73-50 leads to slow field establishment and can extend the crop cycle. Our objective was to determine the cause of this reduced rooting and evaluate treatments to increase the rooting rate. Rooting trials in moist, coarse vermiculite showed that larger slips and green slips with red hues also had a greater number of roots compared with smaller slips and green or yellow slips. Delaying harvesting of the slips after the fruit were harvested also resulted in a greater number of roots. Treatments including components frequently used for rooting cuttings did not significantly increase root numbers. An exception was a tendency for slips treated with potassium nitrate to have greater rooting during some tests. We present data that support the conclusion that the poor root development is associated with the mechanical impedance of the root from the tightly affixed basal leaf bracts. Removal of the lower ten bracts can lead to greater root numbers. When the slip with the bracts removed was tightly wrapped in plastic wrap and masking tape, rooting was reduced. The sizing and selection of slips that are green with a red hue and collected as late as possible after fruit harvest had the best rooting response.
Magia amorosa promocionada en volantes: ritual del amarre
Authors: Quintero Barrera Rosa Patricia
Published: November 2022

El propósito de este artículo es interpretar antropológicamente los mensajes y las imágenes de los volantes que reparten en las calles para publicitar el consumo de la magia amorosa. En ese sentido, se describe y sistematiza su contenido, se interpreta el papel que desempeñan quienes realizan los rituales y los ideales amorosos que difunden los individuos que comercializan con estos productos culturales. La metodología se centra en el contraste de cien volantes recolectados en algunas ciudades colombianas durante los últimos ocho años. Así, se encontró que la publicidad callejera de la magia amorosa está centrada en el ritual de la liga o el amarre, que no solo es apetecido por sectores populares o por mujeres, sino que este recurso de asistencia amorosa es frecuentado por diversos sectores socioeconómicos, donde cada uno encuentra administradores de lo mágico según sus respectivos capitales culturales. El amor es uno de los ámbitos que merece mayor atención en la vida cotidiana, pues, en términos generales, sostener los vínculos amorosos y sexuales con una pareja en particular es un sofisma de felicidad y de seguridad para muchas personas. El ritual del amarre representa violencias simbólicas relacionadas con la humillación y la dominación. Cabe añadir que este tema es relevante, puesto que innova en el estudio de la magia y de los sincretismos religiosos, pues analiza lo que dicen los volantes que brindan la posibilidad de solucionar el abandono y la infidelidad a través del recurso del amarre en secreto y a distancia.
Vibration-shock behavior analysis of compound planetary gear set based on harmonic balance method
Authors: Shen Xiaofang , Liu Kefei , Yang Chao , Zhang Haibo
Published: November 2022

Due to the clearance between the mating gears, a dynamic load rises and causes tooth impact and vibration. In the compound planetary gear set, under the interaction of transmission error, meshing stiffness, support stiffness and backlash, the unilateral tooth impact could evolve into bilateral tooth impact, resulting in a worse dynamic behavior. This work reveals the influence of internal excitations and external excitations on vibration-shock behavior, and, for this purpose, a “purely torsional” lumped parameter dynamics model of compound planetary gear set is built and combined with a harmonic balance method. The steady dynamics response under the frequency domain and time domain is given by the numerical solving method, and the results indicated that the bilateral impact occurs due to the transmission error. To avoid the influence of transmission error on the impact at the resonance peak, the input speed of the compound planetary gear set should avoid the resonance range and harmonious response range. To improve the tooth surface contact by increasing the backlash, it is recommended to decrease the initial velocity to below ωmesh = 0.8.
Study on Fe-C-Al three-phase micro-electrolysis treatment of low concentration phosphorus wastewater
Authors: Hu Baoming , Qi Quan , Li Liang , Huan Yongzhao , Liu Zheng , Liu Xuqing
Published: November 2022

In this study, the iron-carbon-aluminum (Fe-C-Al) composite filler was prepared by aluminum modification of conventional iron-carbon (Fe-C) micro-electrolysis with a no-burn method. The optimal process conditions for Fe-C-Al three-phase micro-electrolysis treatment of low concentration phosphorus wastewater were determined to be the aluminum metal ratio of 14 wt% and solids dosing of 30 g/L. Under the optimal process conditions, Fe-C-Al three-phase micro-electrolysis was performed for the treatment of low concentration phosphorus wastewater (LCPW) with continuous experiment, while iron-carbon fillers before and after treatment were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results showed that the amount of Fe2+ dissolved in the micro-electrolysis determined the micro-electrolysis phosphorus removal effect, Al promoted the dissolution of Fe2+, and the Fe-C-Al filler had a stable phosphorus removal effect, and the average removal efficiency of phosphorus was 67.40%, which is an average improvement of 29.25% compared with the conventional Fe-C filler. The treatment of LCPW by Fe-C-Al three-phase micro-electrolysis is consistent with a first-order kinetic reaction with apparent activation energy of 38.70 kJ·mol−1, which is controlled by the chemical reaction.
N-Glycosylation of the Fourth Repeat Unit of Human τ Protein Abolishes binding to the c-terminal acidic 1-binding segment of ­ βtubulin
Authors: Otvos Laszlo , Pease Anne Marie , Wade John D. , Hoffmann Ralf
Published: November 2022

Tubulin binds to the 18-arnino acid repeat units of normal 1 protein through several sites. Fluorescence polarization showed 1 repeats 1, 3, and 4 bound to an acidic dodecapeptide fragment of tubulin in mM concentrations. From the proposed abnormal modifications of 1 in Alzheimer's disease, only addition of an N-acetyl-glucosarnine moiety to the potential N-glycosylation site Asn359 abolished the 1-tubulin interaction indicating the seminal role of overutilization of this site during the development of the paired helical filaments.
Designing β-hairpin forming short peptide
Authors: Yinlin Sha , Yongliang Huang , Qi Wang , Luhua Lai
Published: November 2022

The short linear peptide sequence H2N-Val-Thr-Leu-D-Pro-Gly-Val-Thr-Val-COOH predicted to form β-hairpin was synthesized by Fmoc/But strategy and purified by RP-HPLC. The CD spectra show a minimum at ca. 216nm and a maximum at ca. 194nm, which have been concluded as the common contribution from β-turn and β-sheet, and the structure in water was enhanced by organic reagent, such as TFE, acetonitrile and isopropyl alcohol.
New synthetic peptides on nuclear localization signal derived from jun by single amino acid substitutions
Authors: Chida Kazuhiro , Tagawa Tomoko , Nagamori Shushi , Kuroki Toshio
Published: November 2022

We examined nuclear transport activities of synthetic peptides on the basis of nuclear localization signal (NLS) of Jun, Arg-Lys-Arg-Lys-Leu, by microinjection into the cytoplasm of NRK cells. Each amino acids of this sequence was replaced by protein or nonprotein amino acids. The four basic amino acids could be replaced by either lysine, arginine or omithine (Om). Replacement of the leucine by a methionine enhanced the transport activity. A nonprotein amino acid sequence Om-Om-Om-Om-Nrv (norvaline) functioned as a NLS.
The promiscuous active site specificity /binding preferences of the fungal aspartic proteinase, rhizopuspepsin
Authors: Lowther W. Todd , Dunn Ben M.
Published: November 2022

Rhizopuspepsin, a secreted proteinase from the fungus Rhizopus chinensis, has been used as a model system for studying the active site interactions of the aspartic proteinases. Substrates containing systematic substitutions at P5-P1 and P2-P1 were analyzed in an effort to identify molecular interactions that could be exploited in structure-based drug design. The recombinant enzyme showed a high degree of kinetic similarity to naturally occurring isozymes. Rhizopuspepsin was able to cleave substrates with side chains varying widely in size, shape, charge and hydrophobicity. Specificity constant (k,,/Km) values (0.06 to 3.84 s·1µM·1) were consistently two to five-fold higher than for the same peptides studied with the mammalian enzymes, porcine pepsin, cathepsin D and cathepsin E. The structural basis for this broad specificity, particularly in the S, subsite, was rationalized to be a result of glycine residues present at positions 290 and 292.
Outlook of Ferroptosis-Targeted Lipid Peroxidation in Cardiovascular Disease
Authors: Wu Ze-Fan , Liu Xi-Yan , Deng Nian-Hua , Ren Zhong , Jiang Zhi-Sheng
Published: November 2022

Lipid metabolism is a complex biochemical process that regulates normal cell activity and death. Ferroptosis is a novel mode of programmed cell death different from apoptosis, pyroptosis, and autophagy. Abnormal lipid metabolism may lead to lipid peroxidation and cell rupture death, which are regulated by lipoxygenase (LOX), long-chain acyl-coA synthases, and antioxidant enzymes. Alternatively, Fe2+ and Fe3+ are required for the activity of LOXs and ferroptosis, and Fe2+ can significantly accelerate lipid peroxidation in ferroptosis. Abnormal lipid metabolism is a certain risk factor for cardiovascular disease. In recent years, the important role of ferroptosis in developing cardiovascular disease has been increasingly reported. Reducing lipid accumulation could reduce the occurrence of ferroptosis, thus alleviating cardiovascular disease deterioration. This article reviews the relationship of lipid peroxidation to the general mechanism of ferroptosis and highlights lipid peroxidation as the common point of ferroptosis and cardiovascular disease.
Implementation of Quality by Design Approach for Optimization of
RP-HPLC Method for Quantification of Abiraterone Acetate in Solid
Dispersion in Forced Degradation Studies
Authors: Choudhari Manisha , Donthi Mahipal Reddy , Damle Shantanu , Singhvi Gautam , Saha Ranendra Narayan , Dubey Sunil Kumar
Published: November 2022

Background:

Abiraterone acetate is a derivative of steroidal progesterone, used as a first-line therapy for metastatic castration of prostate cancer.

Objective:

The present study encompasses the design of an experiment approach for developing a simple, reliable, and rapid RP-HPLC method for the estimation of abiraterone acetate.

Method:

The chromatographic separation was efficiently conducted on a Hypersil Gold C18 (50 x 4.6 mm, 5 µm) HPLC column, using the mobile phase composition of acetonitrile: dibasic potassium phosphate (0.01 mM) in the ratio of 80:20 (%v/v) at pH 6.5 with an isocratic elution mode. Furthermore, the different force degradation study including hydrolysis, oxidation, thermal, and photolytic was performed for abiraterone acetate.

Result:

The dynamic linearity was established in the concentration range of 0.5-10 µg/mL with r2 of 0.9998. Furthermore, the limit of detection and the limit of quantitation were 0.0978 µg/mL and 0.3260 µg/mL. The degradation of abiraterone acetate was shown in both acidic (54.16 ± 0.247 after 24 hrs) and basic conditions (35.06 ± 0.458 after 24 hrs). Furthermore, the developed method was successfully employed to quantify abiraterone acetate in bulk powder and the solid dispersion did not show any change in the retention time.

Conclusion:

The developed method was validated according to the ICH Q2 (R1) specification, which was found to be sensitive, accurate, precise, robust, linear, and selective compared to the reported chromatographic method.
Thermoplastic Polyurethane Graphene Nanocomposites for EMI Shielding
Authors: Verma Meenakshi , Choudhary Veena , Dhawan S.K.
Published: November 2022

Interference and chaos among the various electromagnetic signals are becoming the primary challenge of the current era that relies on wireless communication. Electromagnetic pollution is the overabundance of electromagnetic radiation emitted by electronic devices, like cell phones, cordless phones, Wi-Fi routers, or Bluetooth-enabled equipment, and our relationship with these devices has become more and more intimate. The potential effects of electromagnetic pollution, both in terms of its interaction with electronic devices as well as biological species, are serious concerns for the research community. EMI shielding reduces electromagnetic interference among the electronic components. Therefore, protection from such harmful radiations must be acquired by either blocking or shielding these unavoidable severe electromagnetic radiations. Metals have been typically used as the material of choice for shielding applications, but heavy weight, corrosion susceptibility, and cumbersome processing methods make them unsuitable for both researchers and users. Alternatively, polymer nanocomposites have gained tremendous attention as electromagnetic interference (EMI) shielding materials owing to their facile synthesis, ease of processing, and low cost. Different thermoplastic and thermoset polymer matrices have been explored for the development of lightweight composite material for EMI shielding applications. Among the thermoplastic polymers, thermoplastic polyurethanes (TPU) have attracted a great deal of recognition due to their combination of properties, such as flexibility, stretchability, transparency, good wear and weather resistance, better abrasion and chemical resistance, and better mechanical properties. Although graphene and carbon nanotubes have been explored as conducting fillers in polyurethane matrix for the development of EMI shields, no reports are available using a combination of these fillers along with magnetic nanoparticles in thermoplastic polyurethane matrix.
Utilization of Fly Ash Composites in Electromagnetic Shielding Applications
Authors: Varshney Swati , Dhawan S.K.
Published: November 2022

Electromagnetic interference (EMI) disturbs the working of electronic and
electrical equipment used in aerospace, military, and many more areas. This
disturbance leads to the complete failure of equipment and it is also very dangerous
to human beings, especially radiation created by mobile phones. The considerable
development in materials has been achieved with the fabrication of the shield in the
form of composite sheets, paints, coatings, etc. Now, there is a need to fabricate
flexible materials to achieve intricate shapes and structures to provide excellent EMI
shielding in a wide frequency range. In addition to EM pollution, society is also
dealing with pollution created by solid waste like fly ash. The disposal of fly ash has
become a challenge due to its astounding amount produced in coal thermal power
plants. This research work demonstrates the usage of fly ash to fabricate advanced
composites for electromagnetic shielding applications. This chapter will throw some
light on the electromagnetic shielding mechanism, EMI shielding measurement
methods, fabrication of smart materials for shielding application in the designing of
conducting polypyrrole nanocomposites, polyurethane composites, and cement paint
composite using fly ash along with other magnetic/dielectric reinforcement to develop
material offering optimized electromagnetic shielding properties. Moreover, these
composites are further tested for other characterization techniques. These developed
smart materials not only find a solution for the utilization of fly ash but also offer
excellent shielding effectiveness in a wide frequency range.
Nanostructured Two-Dimensional (2D) Materials as Potential Candidates for EMI Shielding
Authors: Saini Ayushi , Ohlan Anil , Dhawan S. K. , Singh Kuldeep
Published: November 2022

For an effective EMI shielding, materials should have high electrical
conductivity as EMI attenuation is a sum of relfection, absorption, and multiple
relfections which requires the existence of mobile charge carriers (electrons or holes),
electric and/or magnetic dipoles, usually provided by materials having high dielectric
constants (ε) or magnetic permeability (μ) and the large surface area or interface area.
Until now, a metal shroud was the material of choice as an EMI shield. However,
metal fillers add additional weight and are susceptible to corrosion, making them less
desirable. Therefore, we have focused on new emerging two-dimensional 2D
nanomaterials that are light in weight and have a low cost. Here, the focus is to address
the challenges in their synthesis especially transition metal carbides (MXenes), MoS2,
functionalized graphene/ferromagnetic conducting polymer composites, and their
fabrication for EMI reductions. These articles also evaluate and explain the recent
progress explicitly and underline the complex interplay of its intrinsic properties of
2D nanostructured materials (MXene, MoS2, Graphene/ferromagnetic polymer
composite) as a potential candidate for EMI shielding and evaluate their
electromagnetic compatibility. The chapter will cover the facets related to a newly
emerging area of EMI shields in the automotive industry, especially lithium-ion
battery-operated electric vehicles and self-driving cars, high-speed wireless
communication devices, and next-generation mobile phones with 4G and 5G
technology.
Spatio-temporal dynamics of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) on Murraya paniculata (L.) Jack in Cuitláhuac, Veracruz
Authors: Hernández-Landa Lizbeth , López-Collado José , García-García Carlos G. , Osorio-Acosta Francisco , Nava-Tablada Martha E.
Published: November 2022

Orange jasmine (Murraya paniculata) is a garden host plant of Diaphorina citri, the vector of the pathogen that causes Huanglongbing, a lethal citrus disease. It is therefore important to know how vector populations fluctuate in urban areas over time, in order to develop control and management strategies. Here, we studied the spatio-temporal dynamics of D. citri on M. paniculata in Cuitláhuac, Veracruz by placing yellow sticky traps on M. paniculata shrubs from February 2011 to January 2012, located on sidewalks or in house garden across the urban area. Adult psyllid captures were used to create isodensity maps to show the spatial arrangement of insect populations and calculate some aggregation indices. D. citri was present on M. paniculata shrubs throughout the whole sampling period. The results indicated that the highest abundances occurred downtown and at the periphery during March and July. The spatial arrangement of D. citri was aggregated according to the Green index, mean crowding, variance-mean relationship, the Taylor Power Law and k of the negative binomial. This aggregated pattern was density-dependent, that is, the higher the population density, the higher the aggregation. We discuss the risk of occurrence of D. citri in urban areas as a source of infestation for citrus nearby plantations and packing warehouses.
Crabronidae (Hymenoptera) from the locality Cañón del Novillo, Victoria, Tamaulipas, Mexico
Authors: Horta-Vega Jorge Víctor , Vanoye-Eligio Maximiliano , García-Gutiérrez Mauricio Emanuel , Coronado-Blanco Juana María , Barrientos-Lozano Ludivina
Published: November 2022

A total of 67 species and 23 genera of Crabronidae (Hymenoptera) are recorded from 339 specimens collected in 2.3 ha of a spiny shrub located in Cañón del Novillo in Victoria, Tamaulipas, México. The high species richness and diversity index found for this group are comparable with the ones obtained for other hymenopteran taxa from the same locality. Ten new records for Crabronidae arereported for Tamaulipas, increasing to 128 the number of known species of this family of solitary wasps for this state.