bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Transducer Elements Based on Different Material Configurations for Aptamer-Based Electrochemical Biosensors
Authors: Lopez Carrasco Ivan , Cuniberti Gianaurelio , Opitz Jörg , Beshchasna Natalia
Published: July 2024

The selection of an appropriate transducer is a key element in biosensor development. Currently, a wide variety of substrates and working electrode materials utilizing different fabrication techniques are used in the field of biosensors. In the frame of this study, the following three specific material configurations with gold-finish layers were investigated regarding their efficacy to be used as electrochemical (EC) biosensors: (I) a silicone-based sensor substrate with a layer configuration of 50 nm SiO/50 nm SiN/100 nm Au/30–50 nm WTi/140 nm SiO/bulk Si); (II) polyethylene naphthalate (PEN) with a gold inkjet-printed layer; and (III) polyethylene terephthalate (PET) with a screen-printed gold layer. Electrodes were characterized using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) to evaluate their performance as electrochemical transducers in an aptamer-based biosensor for the detection of cardiac troponin I using the redox molecule hexacyanoferrade/hexacyaniferrade (K3[Fe (CN)6]/K4[Fe (CN)6]. Baseline signals were obtained from clean electrodes after a specific cleaning procedure and after functionalization with the thiolate cardiac troponin I aptamers “Tro4” and “Tro6”. With the goal of improving the PEN-based and PET-based performance, sintered PEN-based samples and PET-based samples with a carbon or silver layer under the gold were studied. The effect of a high number of immobilized aptamers will be tested in further work using the PEN-based sample. In this study, the charge-transfer resistance (Rct), anodic peak height (Ipa), cathodic peak height (Ipc) and peak separation (∆E) were determined. The PEN-based electrodes demonstrated better biosensor properties such as lower initial Rct values, a greater change in Rct after the immobilization of the Tro4 aptamer on its surface, higher Ipc and Ipa values and lower ∆E, which correlated with a higher number of immobilized aptamers compared with the other two types of samples functionalized using the same procedure.
Magnetic Immunoassay Based on Au Pt Bimetallic Nanoparticles/Carbon Nanotube Hybrids for Sensitive Detection of Tetracycline Antibiotics
Authors: Lv Jianxia , Huang Rui , Zeng Kun , Zhang Zhen
Published: July 2024

Misusage of tetracycline (TC) antibiotics residue in animal food has posed a significant threat to human health. Therefore, there is an urgent need to develop highly sensitive and robust assays for detecting TC. In the current study, gold and platinum nanoparticles were deposited on carbon nanotubes (CNTs) through the superposition method (Au@Pt/CNTs-s) and one-pot method (Au@Pt/CNTs-o). Au@Pt/CNTs-s displayed higher enzyme-like activity than Au@Pt/CNTs-o, which were utilized for the development of sensitive magnetic immunoassays. Under the optimized conditions, the limits of detection (LODs) of magnetic immunoassays assisted by Au@Pt/CNTs-s and Au@Pt/CNTs-o against TCs could reach 0.74 ng/mL and 1.74 ng/m, respectively, which were improved 6-fold and 2.5-fold in comparison with conventional magnetic immunoassay. In addition, the measurement of TC-family antibiotics was implemented by this assay, and ascribed to the antibody used that could recognize TC, oxytetracycline, chlortetracycline, and doxycycline with high cross-reactivity. Furthermore, the method showed good accuracy (recoveries, 92.1–114.5% for milk; 88.6–92.4% for pork samples), which also were applied for determination of the targets in real samples. This study provides novel insights into the rapid detection of targets based on high-performance nanocatalysts.
Acute Effects of Caffeine Ingestion on Postural Balance, Functional Capacity and Cognitive Function in Patients with End Stage Renal Disease
Authors: Hassen Hayfa Ben Haj , Ammar Achraf , Machfer Amal , Fkih Nadia , Erriahi Siwar , Hamdi Sirine , Chtourou Hamdi , Bouzid Mohamed Amine
Published: July 2024

Patients with end stage renal disease (ESRD) display many cognitive and physiological alterations resulting from renal failure and physical inactivity. Caffeine intake has been reported to improve cognitive/physical performance in several studies. However, whether the benefits of caffeine intake apply to patients with ESRD remains unknown. The aim of the present study was to explore the effect of caffeine intake on postural balance, cognitive function and functional capacity in patients with ESRD. In a randomized order, 12 patients with ESRD (age: 33.85 ± 8.57 years; Female: 45.5%) performed a battery of tests after either caffeine (CF) (400 mg) or placebo (PLA) ingestion. Postural balance was evaluated using a stabilometric platform. Functional capacity was assessed with the sit-to-stand and up-and-go tests, and for cognitive performances, reaction time test and a vigilance test were used. Results showed a significant improvement in up-and-go test (p = 0.01) and sit-to-stand test performances with CF compared to PLA (p < 0.01). Time of reaction test and vigilance test (p < 0.01) performances were significantly higher with CF. No significant difference was observed in postural balance between CF and PLA. The results of this study suggest that acute caffeine ingestion positively enhances cognitive function and functional capacity in patients with ESRD.
OculoMotor & Vestibular Endurance Screening (MoVES) Normative, Repeatability, and Reliability Data
Authors: Iring-Sanchez Stephanie , Dungan Michaela E. , Jones Andrew , Malakhov Mitchell , Mohan Stuti , Yaramothu Chang
Published: July 2024

This study aims to assess oculomotor and vestibular endurance by utilizing the Oculomotor and Vestibular Endurance Screening (MoVES) assessment in athletes’ pre-season and post-season and after a suspected head injury to detect impairment. Athletes (N = 311, 19.4 ± 1.3 years) were recruited to perform the following seven tasks: (1) horizontal saccades, (2) vertical saccades, (3) vergence jumps, (4) horizontal vestibular-oculomotor reflex (VOR), (5) vertical VOR, (6) amplitude of accommodation (AoA), and (7) near point of convergence (NPC). At pre-season, the observed number of eye movements in 60 s are horizontal saccades (74 ± 13 initial 30 s; 67 ± 11 latter 30 s), vertical saccades (70 ± 13; 66 ± 10), vergence jumps (48 ± 12; 45 ± 13), horizontal VOR (38 ± 11; 38 ± 11), and vertical VOR (8 ± 11; 38 ± 11). These results establish a normative database for eye movements within the MoVES assessment and show consistency in the number of movements from pre-season to post-season. The initial results show a trending decrease in the number of eye movements in the initial days post-head injury, which improves to pre-season measures 14–21 days post-injury. This foundation can be used by future studies to explore the extent of binocular and vestibular endurance dysfunctions caused by head injuries that subside within two weeks.
Chinese Rural Kindergarten Teachers’ Work–Family Conflict and Their Turnover Intention: The Role of Emotional Exhaustion and Professional Identity
Authors: Wang Yingjie , Xia Qianqian , Yue Huilan , Teng Wei
Published: July 2024

The loss of rural kindergarten teachers has become a common social concern in China, which is of great importance to the development of preschool education. This study conducted a survey of 2944 kindergarten teachers in mainland China, to explore the relationship between work–family conflict and turnover intention, the mediating effect of emotional exhaustion, and the moderating effect of professional identity. The study used the work–family conflict questionnaire, the emotional exhaustion scale, the turnover intention questionnaire, and the professional identity questionnaire. The results showed that (1) work–family conflict significantly predicted turnover intention; (2) emotional exhaustion played a mediating role between work–family conflict and turnover intention; and (3) professional identity moderated the latter half path of the mediation model, that is, strong professional identity alleviated the indirect predicting effect of work–family conflict on turnover intention through emotional exhaustion. The results clarified the influencing mechanism of work–family conflict on turnover intention, which could help improve rural preschool teachers’ positive emotions and reducing turnover.
Effect of Superabsorbent Polymers and Presoaked Coarse Recycled Shale Lightweight Aggregates on Relative Humidity Development in Early-Age Concrete
Authors: Hu Nan , Hu Dongkang , Ben Shujun , Chen Shuo , Zhao Haitao
Published: July 2024

Self-desiccation-induced shrinkage may result in cracking at an early age, which is averse to the durability of concrete. Internal curing (IC) agents, such as superabsorbent polymers (SAP), are normally used for moisture regulation and shrinkage reduction. In addition, the make-up of recycled shale lightweight aggregate (RSLA) results in a good absorbing capacity, which makes it a potential candidate for IC. In this paper, the synergistic effect of SAP and RSLA on the relative humidity (RH) variation in early-age concrete under sealed conditions is investigated experimentally in terms of the setting time, relative humidity, and autogenous shrinkage. The results indicate that adding SAP and presoaked RSLA can significantly postpone the initial and final setting times. The initial setting time of RSLA30 and SAP06 is delayed by 127 and 171 min, respectively, compared to the benchmark mixture. In addition, increasing the amounts of SAP and presoaked RSLA can effectively extend the duration of the vapour-saturated stage, reducing the decrease in RH and autogenous shrinkage at 28 days. When the RSLA dosage increases from 0 to 10%, 20%, and 30%, the duration of the vapour-saturated stage is extended by 2, 9.4, and 26 days, respectively. Moreover, due to different water desorption behaviours, more IC water released by RSLA during the initial stage can slow the water release of SAP and lead to a higher RH level at 28 days.
An Assessment of the Seismic Performance of EC8-Compliant CBFs Taking into Account the Role of Soil: A Case Study
Authors: Bosco Melina , Fiamingo Angela , Massimino Maria Rossella , Rossi Pier Paolo
Published: July 2024

Based on criticisms raised in the past by researchers about the effectiveness of the design rules reported in the European seismic code for the design of concentrically braced frames, a new design procedure has been proposed and included in the upcoming version of Eurocode 8. The upcoming version of Eurocode 8 is in the enquiry stage. Hence, it is important to evaluate the effectiveness of the design procedure reported in the code using accurate numerical models and seismic inputs. In the present paper, a four-story building with concentrically braced frames in the chevron configuration is designed according to the upcoming version of Eurocode 8. A seismic performance assessment is carried out by the means of multiple-stripe analyses performed on refined numerical models. The seismic input is defined based on one-dimensional local site response analyses. The numerical analyses prove that the use of local site response analysis to properly account for the soil-filtering effects is of paramount importance, and that the design procedure reported in the upcoming version of Eurocode 8 for chevron concentrically braced frames leads to reasonably low probabilities of exceeding the considered limit states.
High-Temperature Stirring Pretreatment of Waste Rubber Particles Enhances the Interfacial Bonding and Mechanical Properties of Rubberized Concrete
Authors: Jing Yuan , Zhang Chunwei , Arab Ali , Lin Guangyi , Zhao Meng
Published: July 2024

This paper innovatively proposes a method of 180 °C high-temperature stirring pretreatment for waste rubber particles and compares this method with untreated, NaOH-treated, and silane coupling agent KH570-treated waste rubber particles. Fourier-transform infrared spectroscopy, X-ray diffraction analysis, water contact angle measurement, scanning electron microscopy, and energy-dispersive X-ray study are used to investigate the effects and mechanisms of different pretreatment methods on waste rubber particles. The results indicate that compared to NaOH-treated and KH570-treated waste rubber particles, the 180 °C high-temperature-stirred pretreated waste rubber particles show significantly improved cleanliness and form a hard oxide film. The study also investigates the effects of different pretreatment methods on the mechanical properties and interface binding performance of rubber concrete made from pretreated waste rubber particles. The results demonstrate that rubber concrete prepared using 180 °C high-temperature-stirred pretreated waste rubber particles substituting 20% fine aggregate exhibits the best mechanical properties and interface bonding performance. The compressive strength recovery rates after 7 and 28 days are 41.6% and 37.3%, respectively; the split tensile strength recovery rates are 47.3% and 60.6%; the axial compressive strength recovery rates are 34.1% and 18.8%; and the static compression moduli of elasticity recovery rates are 46.8% and 26.3%. High-temperature stirring pretreatment of waste rubber particles is simple to operate and suitable for scaled production. Its pretreatment effect is superior to those of the KH570 and NaOH methods, providing a reference value for the scalable application of waste rubber particles as a substitute for fine aggregate in rubber concrete.
Predicting Land Use Changes under Shared Socioeconomic Pathway–Representative Concentration Pathway Scenarios to Support Sustainable Planning in High-Density Urban Areas: A Case Study of Hangzhou, Southeastern China
Authors: Yao Song , Li Yonghua , Jiang Hezhou , Wang Xiaohan , Ran Qinchuan , Ding Xinyi , Wang Huarong , Ding Anqi
Published: July 2024

Amidst the challenges posed by global climate change and accelerated urbanization, the structure and distribution of land use are shifting dramatically, exacerbating ecological and land-use conflicts, particularly in China. Effective land resource management requires accurate forecasts of land use and cover change (LUCC). However, the future trajectory of LUCC, influenced by climate change and urbanization, remains uncertain. This study developed an integrated multi-scenario framework by combining system dynamics and patch-generating land use simulation models to predict future LUCC in high-density urban regions under various Shared Socioeconomic Pathway (SSP)–Representative Concentration Pathway (RCP) scenarios. The results showed the following: (1) From 2020 to 2050, cultivated land, unused land, and water are projected to decrease, while construction land is expected to increase. (2) Future land use patterns exhibit significant spatial heterogeneity across three scenarios. Construction land will expand in all districts of Hangzhou, particularly in the main urban areas. Under the SSP585 scenario, the expansion of construction land is most significant, while it is the least under the SSP126 scenario. (3) Distinct factors drive the expansion of different land use types. The digital elevation model is the predominant factor for the expansion of forest and grassland, contributing 19.25% and 30.76%, respectively. Night light contributes the most to cultivated land and construction land, at 13.94% and 20.35%, respectively. (4) The average land use intensity (LUI) in central urban districts markedly surpasses that in the surrounding suburban areas, with Xiacheng having the highest LUI and Chun’an the lowest. Under the SSP126 scenario, the area with increased LUI is significantly smaller than under the SSP245 and SSP585 scenarios. These findings offer valuable guidance for sustainable planning and built environment management in Hangzhou and similarly situated urban centers worldwide.
Unique Aspects of Scaffolding Design for the Urgent Seismic Retrofitting of Zagreb Cathedral
Authors: Skejić Davor , Čudina Ivan , Valčić Anđelo , Lazarević Damir
Published: July 2024

The 2020 Zagreb earthquake caused extensive damage to historically significant buildings, including the Cathedral of the Assumption of Mary, a key monument of Croatian cultural heritage. The earthquake rendered the cathedral unsafe due to significant damage to its towers and numerous cracks in its vaults and walls. Urgent measures were necessary to ensure safety and facilitate planned reconstruction and strengthening efforts. A specialised scaffolding design was developed to support inaccessible and unstable areas, involving over 1000 tonnes of steel. This paper details the structural concepts and specific scaffolding design implemented for the urgent restoration and seismic retrofitting of Zagreb Cathedral, ensuring it meets high earthquake resistance requirements.
Improving Shear Strength Prediction in Steel Fiber Reinforced Concrete Beams: Stacked Ensemble Machine Learning Modeling and Practical Applications
Authors: Albidah Abdulrahman S. , Abbas Yassir M.
Published: July 2024

Existing machine learning (ML) models often encounter challenges in accurately predicting the shear strength of steel fiber reinforced concrete (SFRC) beams, mainly due to a lack of generalization. This study introduces an advanced stacked ensemble ML architecture to overcome this limitation by utilizing a comprehensive data set of 394 experimental observations and a 20-feature matrix. The model exhibits exceptional performance with a mean absolute error of 0.391 and a correlation coefficient (R2) of 93.7%, and surpasses traditional ML algorithms. Furthermore, a sensitivity analysis of the developed model yields that shear strength is highly responsive to the shear span-to-effective depth ratio, with an increase from 1 to 4 resulting in a significant reduction (about 50%) in strength. Increasing the percentage of longitudinal steel from 1 to 2% leads to a 14.6% gain, whereas doubling its yield strength has a more modest 3.7% effect. Increasing the compressive strength of concrete from 25 to 50 MPa, notably increases the shear strength by 19.6%. Fiber length, diameter, and aspect ratio exhibit varying impacts, with shear strength most influenced by the fiber volume fraction, which leads to a peak enhancement of 30.7% at 2% fibrous volume; however, the tensile strength of fibers minimally affects the shear strength. Additionally, this research presents a simplified empirical model to predict the shear strength of SFRC beams based on the key determinants. This model employs the iterative Gauss–Newton algorithm, demonstrates reasonable predictive capability, and boasts an R2 of 83.3% and mean prediction-tested strengths of around 1.039. The practical implications of these findings are substantial for the construction industry as they enable a more accurate and reliable design of SFRC beams, optimize material usage, and potentially reduce construction costs as well as enhance structural safety.
Research on the Spatiotemporal Pattern and Influencing Mechanism of Coastal Urban Vitality: A Case Study of Bayuquan
Authors: Hu Chaonan , Xu Lei , Cai Xindong , Tian Dongwei , Zhuang Shao
Published: July 2024

Enhancing the spatial vitality of coastal cities is beneficial for the sustainable development of urban construction. However, how to fully utilize coastal resources and boost urban vitality is an important issue. This study takes the coastal city of Bayuquan in China’s cold region as an example. Firstly, we conducted field investigations and data mining in Bayuquan, utilizing Baidu heat map data to measure the spatial–temporal vitality of different areas in Bayuquan. Secondly, we used Moran’s I test to examine the spatial autocorrelation of coastal spatial vitality. Lastly, with the help of the OLS and GWR models, we explored the factors influencing spatial vitality and the urban built environment. The research findings indicate the following: (1) There are spatial–temporal differences in the vitality of different areas in Bayuquan, heavily influenced by the tourist season. (2) The OLS results show that the impact of the built environment on spatial vitality exhibits spatial heterogeneity during different tourist seasons. However, we found no spatial heterogeneity in the influencing factors in the harbor district. (3) The harbor district and the tourism-driven district re quire differentiated construction guidance. Facility functions and block morphology mainly influence the vitality of the harbor district, while the vitality of the tourism-driven district is primarily affected by its aesthetic characteristics. This study can propose differentiated regional construction guidance and specific feasible coastal urban design strategies for seasonally influenced coastal city construction. It holds significant implications for improving urban living quality and is vital to urban decision-makers, planners, and stakeholders.
Effect of Aggregate-to-Binders Ratio on Water Resistance of Red-Mud-Modified Magnesium Phosphate Repair Mortar
Authors: Zhang Maoliang , Zhang Xiaorong , Li Jianwei , Ma Yan , Zhu Zheyu , Liu Junxia
Published: July 2024

The aggregate-to-binders ratio (A/Bs) is an important parameter for the design and preparation of repair mortars. In this paper, the influences of A/Bs on the physical and mechanical properties of red-mud-modified magnesium phosphate repair mortar (RMPM) were systematically investigated. By exploring the capillary absorption characteristics of RMPM, the effect mechanism of A/Bs on its water resistance and mechanical properties was further clarified. The results indicated that the fluidity of fresh RMPM reduced with an increase in A/Bs, and its setting time was first shortened and then prolonged. The compressive strength, flexural strength, interfacial bonding strength, and water resistance of RMPM increased and then decreased with the increasing A/Bs and reached the maximum when the A/Bs was 1.0. The capillary absorption of RMPM was a linear correlation with the square root of the immersion time, and whose slope, that is, the capillary absorption coefficient, and capillary porosity decreased and then increased with the increase in A/Bs. Capillary porosity had a linear relationship with the strength retention rate, which indicated that A/Bs produced a significant effect on the water resistance of RMPM by modifying its capillary pore characteristics. When the A/Bs was 1.0, RMPM had the lowest capillary absorption coefficient and capillary porosity, and thus possesses appropriate mechanical properties and water resistance.
Comprehensive Study of Residential Environment Preferences and Characteristics among Older Adults: Empirical Evidence from China
Authors: Xu Shipeng , Zhang Tao , Fukuda Hiroatsu , He Jiahao , Bao Xin
Published: July 2024

Aging in a suitable residential environment is essential for the health and well-being of older adults. This study aims to analyze the residential environment preferences (REPs) of older people in China to create a residential environment suitable for their physical and mental health, enhancing their life satisfaction. This study used a sample questionnaire to identify relevant characteristics and analyze preferences, which were validated using non-parametric tests and Pearson’s correlation coefficient tests. The questionnaire consisted of 33 questions on characteristics of the residential environment on a 7-point Likert scale and was administered to 433 older adults aged 60 and over in 28 provinces in China. The results showed that “community safety” was the most important environmental characteristic, with an average importance rating of 5.77 out of 6. Accessible building design (average rating of 4.91), emergency response systems (average rating of 4.49), and indoor thermal comfort (average rating of 4.45) were also key factors in promoting aging in place. There was a positive correlation between the community environment and the indoor environment (e.g., community safety and indoor sound insulation, r = 0.209, p < 0.01), and both were, to some extent, negatively correlated with building features (e.g., public toilets and private courtyards, r = −0.278, p < 0.01; indoor thermal comfort and green building design, r = −0.165, p < 0.01). Age and physical health had a strong influence on preferences, but gender had little influence. This study paves the way for future research and policy development on age-friendly housing to ensure sustainable and supportive residential environments for the aging population.
Intelligent Identification of Surrounding Rock Grades Based on a Self-Developed Rock Drilling Test System
Authors: Liu Quanwei , Yan Junlong , Li Hongzhao , Zhang Peiyuan , Liu Yankai , Liu Linsheng , Ye Shoujie , Liu Haitao
Published: July 2024

The classification of surrounding rock is crucial for formulating safe tunnel construction plans and support measures. However, the complex geological environment of tunnels presents a challenge in obtaining accurate drilling parameters for rock mass classification. This paper presents the development of a rock drilling testing system, which includes a propulsion speed acquisition system, oil pressure acquisition system, air pressure acquisition system, and an automatic data acquisition system. This system enables real-time, high-precision automatic collection and storage of parameters such as propulsion speed, with data collected twice per second for each parameter. Leveraging the Qingdao Metro Line 6 as a case study, we conducted rock mass drilling and constructed a rock mass classification database. By employing kernel density estimation and Pearson correlation analysis, we quantified the correlation between rock mass classification and the drilling parameters. The results indicated that relying on a single drilling parameter is insufficient for accurately determining rock mass classification. Both impact pressure and rotational pressure showed the strongest correlation with rock mass classification, each with a correlation coefficient below −0.8 (indicating a strong negative correlation). Outlier values of drilling parameters were excluded using the interval method. Based on the remaining data, we established an intelligent rock mass classification model using the random forest algorithm. This model demonstrated good accuracy and generalization performance, with an average accuracy exceeding 0.9. The proposed rock drilling testing system, combined with the intelligent rock mass classification model, forms an integrated system for the intelligent identification of rock mass grades. This system has significant implications for the intelligent and safe construction of drill-and-blast tunnels.
Analysis and Comparison of Three Bending Tests on Phosphogypsum-Based Material According to Peridynamic Theory
Authors: Ma Haoyu , Zhang Kai , Liang Sheng , Dong Jiatian , Fan Xiangyang , Zhang Xuemei
Published: July 2024

Phosphogypsum-based materials have gained much attention in the field of road infrastructure from the economic and sustainable perspectives. The Three-point bending test, the Four-point bending test and the Semi-circular bending test are three typical test methods applied for fracture energy measurement. However, the optimal test method for fracture energy evaluation has not been determined for phosphogypsum-based materials. To contribute to the gap, this study aims to analyze and compare the three test methods for fracture energy evaluation of phosphogypsum materials based on the peridynamic theory. For this purpose, the load–displacement, vertical displacement–Crack Mouth Opening Displacement (CMOD) and fracture energy of the phosphogypsum-based materials were measured and calculated from the three test methods. The simulated load–displacement and vertical displacement–CMOD by PD numerical models, with different fracture energy as inputs, were compared to the corresponding tested values according to simulation error results. The results showed that the Four-point bending test led to minimized errors lower than 0.189 and indicators lower than 0.124, demonstrating the most optimal test method for the fracture energy measurement of phosphogypsum-based material. The results of this study can provide new methodological references for the selection of material fracture energy measurement tests.
Roof Shape Design for Ice Rinks in Cold Regions under Carbon Reduction Targets
Authors: Wang Lianzi , Pan Wente , Yu Muhan , Liu Chang , Ban Yu
Published: July 2024

In the midst of today’s energy crisis, carbon emissions from ice rinks in cold regions present a significant environmental challenge. The shape of an ice rink’s roof significantly influences these emissions. This study developed a methodology to quantify the carbon emissions of ice rinks and explained how their roof shapes impact emissions during the operational phase. Roof shapes were divided into the following three categories: flat, curved, and combined torsion shell. Carbon emission modeling was established and calibrated using the Ladybug + Honeybee platform, followed by regression analyses on the slope and curvature of each roof type. The findings indicate a robust correlation between the carbon emissions of an ice rink and the slope and curvature of its roof. Roof shape influences approximately 2% of carbon emissions during the operational phase of an ice rink. Among the various roof shapes, the curved dome roof demonstrates the most effective overall carbon savings, at a rate of 0.93% compared to the flat roof. Selecting an appropriate roof shape has significant carbon-saving potential for ice rinks. The findings of this study may serve as a valuable reference for the formulation of energy-saving design standards in cold regions.
Target-Driven Tissue-Agnostic Drug Approvals—A New Path of Drug Development
Authors: Thein Kyaw Z. , Myat Yin M. , Park Byung S. , Panigrahi Kalpana , Kummar Shivaani
Published: July 2024

The regulatory approvals of tumor-agnostic therapies have led to the re-evaluation of the drug development process. The conventional models of drug development are histology-based. On the other hand, the tumor-agnostic drug development of a new drug (or combination) focuses on targeting a common genomic biomarker in multiple cancers, regardless of histology. The basket-like clinical trials with multiple cohorts allow clinicians to evaluate pan-cancer efficacy and toxicity. There are currently eight tumor agnostic approvals granted by the Food and Drug Administration (FDA). This includes two immune checkpoint inhibitors, and five targeted therapy agents. Pembrolizumab is an anti-programmed cell death protein-1 (PD-1) antibody that was the first FDA-approved tumor-agnostic treatment for unresectable or metastatic microsatellite instability-high (MSI-H) or deficient mismatch repair (dMMR) solid tumors in 2017. It was later approved for tumor mutational burden-high (TMB-H) solid tumors, although the TMB cut-off used is still debated. Subsequently, in 2021, another anti-PD-1 antibody, dostarlimab, was also approved for dMMR solid tumors in the refractory setting. Patients with fusion-positive cancers are typically difficult to treat due to their rare prevalence and distribution. Gene rearrangements or fusions are present in a variety of tumors. Neurotrophic tyrosine kinase (NTRK) fusions are present in a range of pediatric and adult solid tumors in varying frequency. Larotrectinib and entrectinib were approved for neurotrophic tyrosine kinase (NTRK) fusion-positive cancers. Similarly, selpercatinib was approved for rearranged during transfection (RET) fusion-positive solid tumors. The FDA approved the first combination therapy of dabrafenib, a B-Raf proto-oncogene serine/threonine kinase (BRAF) inhibitor, plus trametinib, a mitogen-activated protein kinase (MEK) inhibitor for patients 6 months or older with unresectable or metastatic tumors (except colorectal cancer) carrying a BRAFV600E mutation. The most recent FDA tumor-agnostic approval is of fam-trastuzumab deruxtecan-nxki (T-Dxd) for HER2-positive solid tumors. It is important to identify and expeditiously develop drugs that have the potential to provide clinical benefit across tumor types.
Segmentation-Free Outcome Prediction from Head and Neck Cancer PET/CT Images: Deep Learning-Based Feature Extraction from Multi-Angle Maximum Intensity Projections (MA-MIPs)
Authors: Toosi Amirhosein , Shiri Isaac , Zaidi Habib , Rahmim Arman
Published: July 2024

We introduce an innovative, simple, effective segmentation-free approach for survival analysis of head and neck cancer (HNC) patients from PET/CT images. By harnessing deep learning-based feature extraction techniques and multi-angle maximum intensity projections (MA-MIPs) applied to Fluorodeoxyglucose Positron Emission Tomography (FDG-PET) images, our proposed method eliminates the need for manual segmentations of regions-of-interest (ROIs) such as primary tumors and involved lymph nodes. Instead, a state-of-the-art object detection model is trained utilizing the CT images to perform automatic cropping of the head and neck anatomical area, instead of only the lesions or involved lymph nodes on the PET volumes. A pre-trained deep convolutional neural network backbone is then utilized to extract deep features from MA-MIPs obtained from 72 multi-angel axial rotations of the cropped PET volumes. These deep features extracted from multiple projection views of the PET volumes are then aggregated and fused, and employed to perform recurrence-free survival analysis on a cohort of 489 HNC patients. The proposed approach outperforms the best performing method on the target dataset for the task of recurrence-free survival analysis. By circumventing the manual delineation of the malignancies on the FDG PET-CT images, our approach eliminates the dependency on subjective interpretations and highly enhances the reproducibility of the proposed survival analysis method. The code for this work is publicly released.
Patterns and Frequency of Pathogenic Germline Mutations among Patients with Newly-Diagnosed Endometrial Cancer: The Jordanian Exploratory Cancer Genetics (Jo-ECAG) Endometrial Study
Authors: Abdel-Razeq Hikmat , Bani Hani Hira , Sharaf Baha , Tamimi Faris , Khalil Hanan , Abu Sheikha Areej , Alkyam Mais , Abdel-Razeq Sarah , Ghatasheh Tala , Radaideh Tala , Khater Suhaib
Published: July 2024

Most of endometrial cancers are sporadic, with 5% or less being attributed to inherited pathogenic germline mutations and mostly related to the Lynch syndrome. To our knowledge, this is the first study to investigate patterns and frequencies of germline mutations in patients with endometrial cancer in an Arab region. Consecutive patients with endometrial cancer (n = 130), regardless of their age and family history, were enrolled. Germline genetic testing, using an 84-gene panel, was performed on all. Almost half of the patient population (n = 64, 49.2%) was tested based on international guidelines, while the remaining patients (n = 66, 50.8%) were tested as part of an ongoing universal germline genetic testing program. Among the whole group, 18 (13.8%) patients had positive pathogenic or likely pathogenic (P/LP) germline variants. The most common variants encountered were in MLH1 (n = 4, 22.2%), PMS2 (n = 3, 16.7%), ATM, MSH2, MUTYH, and BRCA2 (n = 2, 11.1% each). In addition, three (2.3%) patients were found to have an increased risk allele of the APC gene. P/LP variants were more common among patients with carcinosarcoma and clear cell carcinoma, younger patients (age ≤ 50 years), and in patients with a non-metastatic disease. We conclude that germline genetic variants, mostly in genes related to the Lynch syndrome, are relatively common among Arab patients with endometrial cancer.
Double Heterozygosity for Germline Mutations in Chinese Breast Cancer Patients
Authors: Kwong Ava , Ho Cecilia Y. S. , Au Chun-Hang , Ma Edmond S. K.
Published: July 2024

Double pathogenic mutations occurring in an individual are considered a rare event. The introduction of a multiple-gene panel at Hong Kong Hereditary Breast Cancer Family Registry has allowed the identification of pathogenic variants in multiple genes, providing more information on clinical management and surveillance to the proband and their family members. Breast cancer patients who are double heterozygous (DH) for different hereditary breast and ovarian cancer syndrome (HBCO)-related genes were identified from a cohort of 3649 Chinese patients. Nine patients (0.25%) were observed to have germline DH mutations in ATM, BRCA1, BRCA2, BRIP1, CDH1, CHEK2, MSH6, PALB2, and TP53. Three probands were diagnosed with unilateral breast cancer, two patients were diagnosed with bilateral breast cancer, and four patients had multiple primary cancers. The median age for breast cancer diagnosis was an early age of 36 years. Chinese DH carriers did not show worse phenotypes or have a significantly downhill clinical presentation. However, seven out of nine (77.8%) of our DH carriers harbored a BRCA1 mutation, and four of them (44.4%) developed bilateral breast cancer, suggesting Chinese DH individuals may have a higher chance of having bilateral breast cancer than other populations (p = 0.0237).
Enhancing Trace Pb2⁺ Detection via Novel Functional Materials for Improved Electrocatalytic Redox Processes on Electrochemical Sensors: A Short Review
Authors: Yang Duowen , Wang Xinyu , Xu Hao
Published: July 2024

The efficient detection of lead ions (Pb2⁺) is significant for environmental protection and public health. Electrochemical detection has emerged as one of the most promising technologies due to its low detection limits, high sensitivity, and cost-effectiveness. However, significant challenges remain, including issues related to sensitivity, selectivity, interference, and the stability of electrode materials. This review explores recent advancements in the field, focusing on integrating novel catalytic materials and innovative sensor construction methods. Particular emphasis is placed on enhancing the electrocatalytic redox processes on sensor surfaces using advanced nanomaterials such as MXenes, ferrite-based nanomaterials, carbon nanomaterials, and metal–organic frameworks (MOFs). Additionally, the role of biomaterials and enzymes in improving electrochemical sensors’ selectivity and anti-interference capabilities is discussed. Despite the impressive low detection limits achieved, real-world applications present additional challenges due to the complex composition of environmental samples. The review concludes with future perspectives on overcoming these challenges by leveraging the unique properties of catalytic materials to develop more effective and reliable electrochemical sensors for trace Pb2⁺ detection.
Electrocatalytic Hydrogen Evolution Reaction of Cobalt Triaryl Corrole Bearing Nitro Group
Authors: Zeng Jie , Cao Xu-You , Xu Shi-Yin , Qiu Yi-Feng , Chen Jun-Ying , Si Li-Ping , Liu Hai-Yang
Published: July 2024

The use of non–precious metals for electrocatalytic hydrogen reaction (HER) is particularly important for energy conservation and environmental protection. In this work, three new cobalt corroles containing o−, m−, and p−nitrobenzyl (1, 2, 3) at the meso 10−position of the corrole macrocycle were synthesized, and their electrocatalytic hydrogen evolution reaction in organic and neutral aqueous systems was also investigated. The results show that these three cobalt corroles have significant catalytic HER activity in both systems, and the catalytic efficiency follows 1 > 3 > 2, which indicates that the position of the nitro group can affect the catalytic property of the complexes. In the organic phase, when using trifluoroacetic acid or p−toluenesulfonic acid as the proton source, the electrocatalytic HER may undergo an EECC (E: electron transfer, C: proton coupling) pathway. In a neutral aqueous system, the HER turnover frequency value of 1 is up to 137.4 h−1 at 938 mV overpotential.
Initial Characterization of WDR5B Reveals a Role in the Proliferation of Retinal Pigment Epithelial Cells
Authors: Bailey Jeffrey K. , Ma Dzwokai , Clegg Dennis O.
Published: July 2024

The chromatin-associated protein WDR5 has been widely studied due to its role in histone modification and its potential as a pharmacological target for the treatment of cancer. In humans, the protein with highest sequence homology to WDR5 is encoded by the retrogene WDR5B, which remains unexplored. Here, we used CRISPR-Cas9 genome editing to generate WDR5B knockout and WDR5B-FLAG knock-in cell lines for further characterization. In contrast to WDR5, WDR5B exhibits low expression in pluripotent cells and is upregulated upon neural differentiation. Loss or shRNA depletion of WDR5B impairs cell growth and increases the fraction of non-viable cells in proliferating retinal pigment epithelial (RPE) cultures. CUT&RUN chromatin profiling in RPE and neural progenitors indicates minimal WDR5B enrichment at established WDR5 binding sites. These results suggest that WDR5 and WDR5B exhibit several divergent biological properties despite sharing a high degree of sequence homology.
An Introductory Guide to Using Bloomington Drosophila Stock Center and FlyBase for Aging Research
Authors: Zheng Xiangzhong
Published: July 2024

Studies on numerous species have demonstrated strikingly conserved mechanisms that determine the aging process, from yeasts to worms, flies, zebrafish, mice, and humans. The fruit fly Drosophila melanogaster is an excellent model organism for studying the biological basis of normal aging and etiology of age-related diseases. Since its inception in 1967, the Bloomington Drosophila Stock Center (BDSC) has grown into the largest collection of documented D. melanogaster strains (currently > 91,000). This paper aims to briefly review conserved mechanisms of aging and provides a guide to help users understand the organization of stock listings on the BDSC website and familiarize themselves with the search functions on BDSC and FlyBase, with an emphasis on using genes in conserved pathways as examples to find stocks for aging studies.
Peptide Blockers of PD-1-PD-L1 Interaction Reinvigorate PD-1-Suppressed T Cells and Curb Tumor Growth in Mice
Authors: Zhong Shanshan (Jenny) , Liu Xiaoling , Kaneko Tomonori , Feng Yan , Hovey Owen , Yang Kyle , Ezra Sally , Ha Soon-Duck , Kim Sung , McCormick John K. , Liu Huadong , Li Shawn Shun-Cheng
Published: July 2024

The programmed cell death protein 1 (PD-1) plays a critical role in cancer immune evasion. Blocking the PD-1-PD-L1 interaction by monoclonal antibodies has shown remarkable clinical efficacy in treating certain types of cancer. However, antibodies are costly to produce, and antibody-based therapies can cause immune-related adverse events. To address the limitations associated with current PD-1/PD-L1 blockade immunotherapy, we aimed to develop peptide-based inhibitors of the PD-1/PD-L1 interaction as an alternative means to PD-1/PD-L1 blockade antibodies for anti-cancer immunotherapy. Through the functional screening of peptide arrays encompassing the ectodomains of PD-1 and PD-L1, followed by the optimization of the hit peptides for solubility and stability, we have identified a 16-mer peptide, named mL7N, with a remarkable efficacy in blocking the PD-1/PD-L1 interaction both in vitro and in vivo. The mL7N peptide effectively rejuvenated PD-1-suppressed T cells in multiple cellular systems designed to recapitulate the PD-1/PD-L1 interaction in the context of T-cell receptor signaling. Furthermore, PA-mL7N, a chimera of the mL7N peptide coupled to albumin-binding palmitic acid (PA), significantly promoted breast cancer cell killing by peripheral blood mononuclear cells ex vivo and significantly curbed tumor growth in a syngeneic mouse model of breast cancer. Our work raises the prospect that mL7N may serve as a prototype for the development of a new line of peptide-based immunomodulators targeting the PD-1/PD-L1 immune checkpoint with potential applications in cancer treatment.
Association of Preoperative Copeptin Levels with Risk of All-Cause Mortality in a Prospective Cohort of Adult Cardiac Surgery Patients
Authors: Filipovic Mark G. , Huber Markus , Kobel Beatrice , Bello Corina , Levis Anja , Andereggen Lukas , Kakizaki Ryota , Stüber Frank , Räber Lorenz , Luedi Markus M.
Published: July 2024

We aimed to investigate the association of preoperative copeptin, a new cardiovascular biomarker, with short- and long-term mortality in a cohort of adult patients undergoing cardiac surgery, including its potential as a prognostic marker for clinical outcome. Preoperative blood samples of the Bern Perioperative Biobank, a prospective cohort of adults undergoing cardiac surgery during 2019, were analyzed. The primary and secondary outcome measures were 30-day and 1-year all-cause mortality. Optimal copeptin thresholds were calculated with the Youden Index. Associations of copeptin levels with the two outcomes were examined with multivariable logistic regression models; their discriminatory capacity was assessed with the area under the receiver operating characteristic (AUROC). A total of 519 patients (78.4% male, median age 67 y (IQR: 60–73 y)) were included, with a median preoperative copeptin level of 7.6 pmol/L (IQR: 4.7–13.2 pmol/L). We identified an optimal threshold of 15.9 pmol/l (95%-CI: 7.7 to 46.5 pmol/L) for 30-day mortality and 15.9 pmol/L (95%-CI: 9.0 to 21.3 pmol/L) for 1-year all-cause mortality. Regression models featured an AUROC of 0.79 (95%-CI: 0.56 to 0.95) for adjusted log-transformed preoperative copeptin for 30-day mortality and an AUROC of 0.76 (95%-CI: 0.64 to 0.88) for 1-year mortality. In patients undergoing cardiac surgery, the baseline levels of copeptin emerged as a strong marker for 1-year all-cause death. Preoperative copeptin levels might possibly identify patients at risk for a complicated, long-term postoperative course, and therefore requiring a more rigorous postoperative observation and follow-up.
Unveiling the Regulatory Role of LncRNA MYU in Hypoxia-Induced Angiogenesis via the miR-23a-3p Axis in Endothelial Cells
Authors: Zhou Xiankun , Wen Mingxing , Zhang Jinwei , Long Keren , Lu Lu , Jin Long , Sun Jing , Ge Liangpeng , Li Xuewei , Li Mingzhou , Ma Jideng
Published: July 2024

Background: Angiogenesis is essential for various physiological and pathological processes, such as embryonic development and cancer cell proliferation, migration, and invasion. Long noncoding RNAs (lncRNAs) play pivotal roles in normal homeostasis and disease processes by regulating gene expression through various mechanisms, including competing endogenous RNAs (ceRNAs) of target microRNAs (miRNAs). The lncRNA MYU is known to promote prostate cancer proliferation via the miR-184/c-Myc regulatory axis and to be upregulated in vascular endothelial cells under hypoxic conditions, which often occurs in solid tumors. In the present study, we investigated whether MYU might affect cancer growth by regulating angiogenesis in vascular endothelial cells under hypoxia. Methods: The expression of MYU-regulated miR-23a-3p and interleukin-8 (IL-8) in HUVEC cell lines was examined using qRT-PCR. The CCK-8 assay, EdU assay, wound-healing assay, and tube-formation assay were used to assess the effects of MYU on cell proliferation, migration, and tube formation of HUVEC cells in vitro. The dual-luciferase reporter assay was performed to examine the effects of miR-23a-3p on MYU and IL-8 expression. Results: We found that the overexpression of MYU and knockdown of miR-23a-3p in human umbilical vein endothelial cells (HUVECs) under hypoxia promoted cell proliferation, migration, and tube formation. Mechanistically, MYU was shown to bind competitively to miR-23a-3p, thereby preventing miR-23a-3p binding to the 3′ untranslated region of IL-8 mRNA. In turn, increased production of pro-angiogenic IL-8 promoted HUVEC proliferation, migration, and tube formation under hypoxia. Conclusion: This study identified a new role for lncRNA MYU as a ceRNA for miR-23a-3p and uncovered a novel MYU–miR-23a-3p–IL-8 regulatory axis for angiogenesis. MYU and/or miR-23a-3p may thus represent new targets for the treatment of hypoxia-related diseases by promoting angiogenesis.