bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Variant c.245A>G (p.Asn82Ser) in GIPC3 Gene Is a Frequent Cause of Hereditary Nonsyndromic Sensorineural Hearing Loss in Chuvash Population
Authors: Petrova Nika V. , Marakhonov Andrey V. , Balinova Natalia V. , Abrukova Anna V. , Konovalov Fedor A. , Kutsev Sergey I. , Zinchenko Rena A.
Published: May 2021

Hereditary nonsyndromic sensorineural hearing loss is a disease in which hearing loss occurs due to damage to the organ of the inner ear, the auditory nerve, or the center in the brain that is responsible for the perception of sound, characterized by wide locus and allelic heterogeneity and different types of inheritance. Given the diversity of population of the Russian Federation, it seems necessary to study the ethnic characteristics of the molecular causes of the disease. The aim is to study the molecular and genetic causes of hereditary sensorineural hearing loss in Chuvash, the fifth largest ethnic group in Russia. DNA samples of 26 patients from 21 unrelated Chuvash families from the Republic of Chuvashia, in whom the diagnosis of hereditary sensorineural hearing loss had been established, were analyzed using a combination of targeted Sanger sequencing, multiplex ligase-dependent probe amplification, and whole exome sequencing. The homozygous variant NM_133261.3(GIPC3):c.245A>G (p.Asn82Ser) is the major molecular cause of hereditary sensorineural hearing loss in 23% of Chuvash patients (OMIM #601869). Its frequency was 25% in patients and 1.1% in healthy Chuvash population. Genotyping of the NM_133261.3(GIPC3):c.245A>G (p.Asn82Ser) variant in five neighboring populations from the Volga-Ural region (Russian, Udmurt, Mary, Tatar, Bushkir) found no evidence that this variant is common in those populations.
Prediction of Eye Colour in Scandinavians Using the EyeColour 11 (EC11) SNP Set
Authors: Meyer Olivia Strunge , Salvo Nina Mjølsnes , Kjærbye Anne , Kjersem Marianne , Andersen Mikkel Meyer , Sørensen Erik , Ullum Henrik , Janssen Kirstin , Morling Niels , Børsting Claus , Olsen Gunn-Hege , Andersen Jeppe Dyrberg
Published: May 2021

Description of a perpetrator’s eye colour can be an important investigative lead in a forensic case with no apparent suspects. Herein, we present 11 SNPs (Eye Colour 11-EC11) that are important for eye colour prediction and eye colour prediction models for a two-category reporting system (blue and brown) and a three-category system (blue, intermediate, and brown). The EC11 SNPs were carefully selected from 44 pigmentary variants in seven genes previously found to be associated with eye colours in 757 Europeans (Danes, Swedes, and Italians). Mathematical models using three different reporting systems: a quantitative system (PIE-score), a two-category system (blue and brown), and a three-category system (blue, intermediate, brown) were used to rank the variants. SNPs with a sufficient mean variable importance (above 0.3%) were selected for EC11. Eye colour prediction models using the EC11 SNPs were developed using leave-one-out cross-validation (LOOCV) in an independent data set of 523 Norwegian individuals. Performance of the EC11 models for the two- and three-category system was compared with models based on the IrisPlex SNPs and the most important eye colour locus, rs12913832. We also compared model performances with the IrisPlex online tool (IrisPlex Web). The EC11 eye colour prediction models performed slightly better than the IrisPlex and rs12913832 models in all reporting systems and better than the IrisPlex Web in the three-category system. Three important points to consider prior to the implementation of eye colour prediction in a forensic genetic setting are discussed: (1) the reference population, (2) the SNP set, and (3) the reporting strategy.
Mutational Asymmetries in the SARS-CoV-2 Genome May Lead to Increased Hydrophobicity of Virus Proteins
Authors: Matyášek Roman , Řehůřková Kateřina , Berta Marošiová Kristýna , Kovařík Aleš
Published: May 2021

The genomic diversity of SARS-CoV-2 has been a focus during the ongoing COVID-19 pandemic. Here, we analyzed the distribution and character of emerging mutations in a data set comprising more than 95,000 virus genomes covering eight major SARS-CoV-2 lineages in the GISAID database, including genotypes arising during COVID-19 therapy. Globally, the C>U transitions and G>U transversions were the most represented mutations, accounting for the majority of single-nucleotide variations. Mutational spectra were not influenced by the time the virus had been circulating in its host or medical treatment. At the amino acid level, we observed about a 2-fold excess of substitutions in favor of hydrophobic amino acids over the reverse. However, most mutations constituting variants of interests of the S-protein (spike) lead to hydrophilic amino acids, counteracting the global trend. The C>U and G>U substitutions altered codons towards increased amino acid hydrophobicity values in more than 80% of cases. The bias is explained by the existing differences in the codon composition for amino acids bearing contrasting biochemical properties. Mutation asymmetries apparently influence the biochemical features of SARS CoV-2 proteins, which may impact protein–protein interactions, fusion of viral and cellular membranes, and virion assembly.
Spatial Analysis of Groundwater Hydrochemistry through Integrated Multivariate Analysis: A Case Study in the Urbanized Langat Basin, Malaysia
Authors: Zainol Nur Fatihah Mohamad , Zainuddin Azim Haziq , Looi Ley Juen , Aris Ahmad Zaharin , Isa Noorain Mohd , Sefie Anuar , Ku Yusof Ku Mohd Kalkausar
Published: May 2021

Rapid urbanization and industrial development in the Langat Basin has disturbed the groundwater quality. The populations’ reliance on groundwater sources may induce possible risks to human health such as cancer and endocrine dysfunction. This study aims to determine the groundwater quality of an urbanized basin through 24 studied hydrochemical parameters from 45 groundwater samples obtained from 15 different sampling stations by employing integrated multivariate analysis. The abundance of the major ions was in the following order: bicarbonate (HCO3−) > chloride (Cl−) > sodium (Na+) > sulphate (SO42−) > calcium (Ca2+) > potassium (K+) > magnesium (Mg2+). Heavy metal dominance was in the following order: Fe > Mn > Zn > As > Hg > Pb > Ni > Cu > Cd > Se > Sr. Classification of the groundwater facies indicated that the studied groundwater belongs to the Na-Cl with saline water type and Na-HCO3 with mix water type characteristics. The saline water type characteristics are derived from agricultural activities, while the mixed water types occur from water–rock interaction. Multivariate analysis performance suggests that industrial, agricultural, and weathering activities have contributed to groundwater contamination. The study will help in the understanding of the groundwater quality issue and serve as a reference for other basins with similar characteristics.
Experimental Investigation of Centrifugal Flow in Rotor–Stator Cavities at High Reynolds Numbers >108
Authors: Schröder Tilman , Schuster Sebastian , Brillert Dieter
Published: May 2021

The designers of radial turbomachinery need detailed information on the impact of the side chamber flow on axial thrust and torque. A previous paper investigated centripetal flow through narrow rotor–stator cavities and compared axial thrust, rotor torque and radial pressure distribution to the case without through-flow. Consequently, this paper extends the investigated range to centrifugal through-flow as it may occur in the hub side chamber of radial turbomachinery. The chosen operating conditions are representative of high-pressure centrifugal compressors used in, for example, carbon capture and storage applications as well as hydrogen compression. To date, only the Reynolds number range up to Re=2·107 has been investigated for centrifugal through-flow. This paper extends the range to Reynolds numbers of Re=2·108 and reports results of experimental and numerical investigations. It focuses on the radial pressure distribution in the rotor–stator cavity and shows the influence of the Reynolds number, cavity width and centrifugal mass flow rate. It therefore extends the range of available valid data that can be used to design radial turbomachinery. Additionally, this analysis compares the results to data and models from scientific literature, showing that in the higher Reynolds number range, a new correlation is required. Finally, the analysis of velocity profiles and wall shear delineates the switch from purely radial outflow in the cavity to outflow on the rotor and inflow on the stator at high Reynolds numbers in comparison to the results reported by others for Reynolds numbers up to Re=2·107.
MMORPG Evolution Analysis from Explorer and Achiever Perspectives: A Case Study Using the Final Fantasy Series
Authors: Wang Haolan , Zhang Zeliang , Khalid Mohd Nor Akmal , Iida Hiroyuki , Li Keqiu
Published: May 2021

Due to the advent of the Internet, massively multiplayer online role-playing games (MMORPGs) have been enjoyed worldwide by many players simultaneously, and game publishers’ revenues have reached billions of dollars from subscriptions alone. Frequent updates (e.g., versioning) and new contents (e.g., quest system) are the typical strategies adopted by developers to keep MMORPG experiences fresh and attractive. What makes such strategies attractive and retains the interest of players in MMORPGs? This study focuses on one aspect of a popular MMORPG: the player’s experience of the quest systems of Final Fantasy XIV (FF14). The different quest systems were analyzed considering Bartle’s players’ classification, specifically for the explorers and achievers. From an information science perspective, such an analysis can be achieved via game refinement (GR) theory, which formulates the information of the game’s progression into a measurable model of game sophistication. On top of that, we used the concept of motion in mind, which was derived from concepts in physics. It maps game progression information to enable the possible quantification and approximation of players’ mental movements and affective experiences in the game. Based on the analysis of the collected data using the proposed measures of GR and motion in mind, the impact of regular updates on players in long-term games is discussed. Insights from the study provide guidance and suggestions for potential improvements in long-term game design.
(Epi)Genetic Mechanisms Underlying the Evolutionary Success of Eusocial Insects
Authors: Sieber Kayli R. , Dorman Taylor , Newell Nicholas , Yan Hua
Published: May 2021

Eusocial insects, such as bees, ants, and wasps of the Hymenoptera and termites of the Blattodea, are able to generate remarkable diversity in morphology and behavior despite being genetically uniform within a colony. Most eusocial insect species display caste structures in which reproductive ability is possessed by a single or a few queens while all other colony members act as workers. However, in some species, caste structure is somewhat plastic, and individuals may switch from one caste or behavioral phenotype to another in response to certain environmental cues. As different castes normally share a common genetic background, it is believed that much of this observed within-colony diversity results from transcriptional differences between individuals. This suggests that epigenetic mechanisms, featured by modified gene expression without changing genes themselves, may play an important role in eusocial insects. Indeed, epigenetic mechanisms such as DNA methylation, histone modifications and non-coding RNAs, have been shown to influence eusocial insects in multiple aspects, along with typical genetic regulation. This review summarizes the most recent findings regarding such mechanisms and their diverse roles in eusocial insects.
A Novel Genetic Sexing Strain of Anastrepha ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency
Authors: Ramírez-Santos Edwin , Rendon Pedro , Gouvi Georgia , Zacharopoulou Antigone , Bourtzis Kostas , Cáceres Carlos , Bloem Kenneth
Published: May 2021

Anastrepha ludens (Loew) is one of the most destructive insect pests damaging several fruits of economic importance. The sterile insect technique (SIT) is used under an area-wide integrated pest management approach, to suppress these pest populations. Mass rearing facilities were initially established to produce sterile males of bi-sexual strains in support of SIT. The first genetic sexing strain (GSS) for A. ludens, Tapachula-7, based on pupal color dimorphism, was a key development since the release of males-only significantly increases the SIT efficiency. In this study, we document the development of a novel pupal color-based GSS. Twelve radiation-induced translocation lines were assessed as potential GSS in terms of recombination rates and rearing efficiency at a small scale. The best one, GUA10, was cytogenetically characterized: it was shown to carry a single translocation between the Y chromosome and chromosome 2, which is known to carry the black pupae marker. This GSS was further evaluated at medium and large scales regarding its genetic stability, productivity and quality versus Tapachula-7. GUA10 presented better genetic stability, fecundity, fertility, production efficiency, flying ability, and male mating, clear indicators that GUA10 GSS can significantly improve the efficacy and cost-effectiveness of SIT applications against this pest species.
The Performance of Filava-Polysiloxane, Silres® H62C Composite in High Temperature Application
Authors: Bari Klaudio , Loganathan Thozhuvur Govindaraman
Published: May 2021

The research aim is to investigate the performance of novel enriched mineral fibres (Filava) in polysiloxane SLIRES H62 resin. Specimens were manufactured using a vacuum bagging process and oven cured at 250 °C. Specimens were prepared for flexural testing according to BS EN ISO 14125:1998 to obtain flexural strength, modulus, and elongation. The mechanical strength was compared to similar composites, with the aim of determining composite performance index. The flexural modulus (9.7 GPa), flexural strength (83 MPa), and flexural strain (2.9%) were obtained from a three-point bending test. In addition, the study investigates the thermal properties of the composite using a state-of-art Zwick Roell high temperature tensile rig. The results showed Filava/Polysiloxane Composites had an ultimate tensile strength 400 MPa, Young’s modulus 16 GPa and strain 2.5% at 1000 °C, and no smoke and ash were observed during pyrolysis. Ongoing research is currently taking place to use Filava-H62 in fire-retardant enclosure for lithium-ferro-phosphate Batteries used in electric trucks.
Fundamental Power of Social Media Interactions for Building a Brand and Customer Relations
Authors: Oncioiu Ionica , Căpușneanu Sorinel , Topor Dan , Tamaș Attila , Solomon Alina-Georgiana , Dănescu Tatiana
Published: May 2021

The spread of social networks and the high level of penetration of digital content and mobile devices have created a society in which consumers expect constant communication from companies. In addition, communication on social media is often accompanied by the hope of being able to interact on an equal footing. In this way, companies hope that the use of social networks will not only increase sales, but also increase attention, brand page traffic, and customer loyalty. The purpose of this study was to provide the grounds for identifying the correlations between the interactive potential of social media based on dialogue-oriented communication and the quality of relationships with online customers. To achieve this goal, an online questionnaire was used in which 604 respondents from Romania participated (204 social media managers and 400 online customers). The results of the study showed that it is particularly important for companies to understand the role of interactive communication in social media and to thus build a bridge between the orientation of communication towards dialogue and relationships with online customers. It was found that the correct use of social networks could also support the achievement of public relation (PR) objectives. In addition, this study has implications for the development of customer-oriented online communication strategies by Romanian companies.
Thyroid-Stimulating Hormone Stimulation Tests in the Bottlenose Dolphin (Tursiops truncatus)
Authors: Houser Dorian S. , Champagne Cory , Crocker Daniel E.
Published: May 2021

Stimulation of the thyroid with thyroid-stimulating hormone (TSH) is a potentially useful diagnostic of thyroid dysfunction, but little is known about the response of the thyroid to TSH stimulation in bottlenose dolphins (Tursiops truncatus). To better characterize the response of the dolphin thyroid to TSH stimulation, five adult dolphins participated in a TSH stimulation study. Dolphins voluntarily beached onto a padded mat and were given a 1.5 mg intramuscular injection of human recombinant TSH. Blood samples collected the day prior, at multiple intervals the day of, and daily for three days after the injection were analyzed via radioimmunoassay for free and total triiodothyronine (fT3 and tT3), and free and total thyroxine (fT4 and tT4). Significant increases in circulating fT3, fT4, and tT4 were observed with peaks occurring for all hormones the day after the TSH injection; maximal increases were 44%, 47%, and 23% for each hormone, respectively. Temporal patterns in the hormones potentially reflected feedback mechanisms countering the surge in fT3 following stimulation. Though recombinant human TSH was effective at stimulating hormone release, it is likely that use of dolphin or dolphin-derived TSH would enhance the clinical utility of the stimulation test, as would the development of antibodies specific to dolphin TSH.
Photocatalytic Technology for Palm Oil Mill Effluent (POME) Wastewater Treatment: Current Progress and Future Perspective
Authors: Saputera Wibawa Hendra , Amri Aryan Fathoni , Daiyan Rahman , Sasongko Dwiwahju
Published: May 2021

The palm oil industry produces liquid waste called POME (palm oil mill effluent). POME is stated as one of the wastes that are difficult to handle because of its large production and ineffective treatment. It will disturb the ecosystem with a high organic matter content if the waste is disposed directly into the environment. The authorities have established policies and regulations in the POME waste quality standard before being discharged into the environment. However, at this time, there are still many factories in Indonesia that have not been able to meet the standard of POME waste disposal with the existing treatment technology. Currently, the POME treatment system is still using a conventional system known as an open pond system. Although this process can reduce pollutants’ concentration, it will produce much sludge, requiring a large pond area and a long processing time. To overcome the inability of the conventional system to process POME is believed to be a challenge. Extensive effort is being invested in developing alternative technologies for the POME waste treatment to reduce POME waste safely. Several technologies have been studied, such as anaerobic processes, membrane technology, advanced oxidation processes (AOPs), membrane technology, adsorption, steam reforming, and coagulation. Among other things, an AOP, namely photocatalytic technology, has the potential to treat POME waste. This paper provides information on the feasibility of photocatalytic technology for treating POME waste. Although there are some challenges in this technology’s large-scale application, this paper proposes several strategies and directions to overcome these challenges.
Comparative Analysis on the Micropore and Microstructure Characteristics of Concrete under Insulated Formwork
Authors: Lim Myung-Kwan , Nam Kyung-Yong
Published: May 2021

During concrete construction in winter, the concrete performance is generally improved by adding a chemical admixture or providing protection using tents and hot-air blowers. However, long-term strength or safety accidents may occur due to the installation and removal of the tents. This study considered insulated gang forms to improve formwork methods. In this regard, the microstructure and micropore characteristics of concrete were investigated experimentally to examine the insulated gang form effect on the physical and mechanical properties of concrete. The micropore characteristics were investigated through scanning electron microscopy. The results confirm that applying insulated gangs improves workability and safety without adding chemical admixture. Moreover, the application of insulated gang forms reduces the use of tents and hot-air blowers. Therefore, insulated gangs provide excellent initial quality to the concrete.
Effect of Particle Size on the Mechanical Properties of TiO2–Epoxy Nanocomposites
Authors: Choi Young-Min , Hwangbo Seon-Ae , Lee Tae Geol , Ham Young-Bog
Published: May 2021

This study investigated the effects of the packing density and particle size distribution of TiO2 nanoparticles on the mechanical properties of TiO2–epoxy nanocomposites (NCs). The uniform dispersion and good interfacial bonding of TiO2 in the epoxy resin resulted in improved mechanical properties with the addition of nanoparticles. Reinforcement nano-TiO2 particles dispersed in deionized water produced by three different ultrasonic dispersion methods were used; the ultrasonication effects were then compared. The nano-TiO2 suspension was added at 0.5–5.0 wt.%, and the mechanical and thermal properties of TiO2–epoxy NCs were compared using a universal testing machine, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC). The tensile strength of the NCs was improved by the dispersion strengthening effect of the TiO2 nanoparticles, and focused sonication improved the tensile strength the most when nano-TiO2 suspensions with a particle size of 100 nm or smaller were used. Thus, the reinforcing effect of TiO2 nanoparticles on the epoxy resin was observed, and the nano-TiO2 suspension produced by focused sonication showed a more distinct reinforcing effect.
Mineralization of Titanium Surfaces: Biomimetic Implants
Authors: Gil Javier , Manero Jose Maria , Ruperez Elisa , Velasco-Ortega Eugenio , Jiménez-Guerra Alvaro , Ortiz-García Iván , Monsalve-Guil Loreto
Published: May 2021

The surface modification by the formation of apatitic compounds, such as hydroxyapatite, improves biological fixation implants at an early stage after implantation. The structure, which is identical to mineral content of human bone, has the potential to be osteoinductive and/or osteoconductive materials. These calcium phosphates provoke the action of the cell signals that interact with the surface after implantation in order to quickly regenerate bone in contact with dental implants with mineral coating. A new generation of calcium phosphate coatings applied on the titanium surfaces of dental implants using laser, plasma-sprayed, laser-ablation, or electrochemical deposition processes produces that response. However, these modifications produce failures and bad responses in long-term behavior. Calcium phosphates films result in heterogeneous degradation due to the lack of crystallinity of the phosphates with a fast dissolution; conversely, the film presents cracks, which produce fractures in the coating. New thermochemical treatments have been developed to obtain biomimetic surfaces with calcium phosphate compounds that overcome the aforementioned problems. Among them, the chemical modification using biomineralization treatments has been extended to other materials, including composites, bioceramics, biopolymers, peptides, organic molecules, and other metallic materials, showing the potential for growing a calcium phosphate layer under biomimetic conditions.
Effectiveness of Floating-Point Precision on the Numerical Approximation by Spectral Methods
Authors: Matos José A. O. , Vasconcelos Paulo B.
Published: May 2021

With the fast advances in computational sciences, there is a need for more accurate computations, especially in large-scale solutions of differential problems and long-term simulations. Amid the many numerical approaches to solving differential problems, including both local and global methods, spectral methods can offer greater accuracy. The downside is that spectral methods often require high-order polynomial approximations, which brings numerical instability issues to the problem resolution. In particular, large condition numbers associated with the large operational matrices, prevent stable algorithms from working within machine precision. Software-based solutions that implement arbitrary precision arithmetic are available and should be explored to obtain higher accuracy when needed, even with the higher computing time cost associated. In this work, experimental results on the computation of approximate solutions of differential problems via spectral methods are detailed with recourse to quadruple precision arithmetic. Variable precision arithmetic was used in Tau Toolbox, a mathematical software package to solve integro-differential problems via the spectral Tau method.
Clinical Outcomes and Risk Factor Analysis of Patients Presenting with Emphysematous Cystitis: A 15-Year Retrospective Multicenter Study
Authors: Choi Jeonghyouk , Choi Seung-Kwon , Lee Sang-Hyub , Yoo Koo-Han
Published: May 2021

Background and objectives: To investigate the risk factors for emphysematous cystitis (EC) compared to those of acute cystitis (AC) to increase clinicians awareness of the possibility for the aggravation of patient status. Materials and methods: We retrospectively reviewed a total of 54 patients who were hospitalized with a diagnosis of EC by abdominal computed tomography (CT) scan from 2006 to 2020. The control group included 92 patients who were hospitalized for the treatment of AC in the same period. We sought to identify the clinical features and predisposing diseases, such as age, sex, diabetes mellitus (DM), hypertension (HTN), cerebrovascular accident (CVA), chronic kidney disease (CKD), neurogenic bladder (NB), history of urinary tract infection (UTI), and emphysematous pyelonephritis (EPN), that were associated with the development of EC. Results: The median (interquartile range (IQR)) age of the patients with EC was older than that of the patients with AC (78.5 (15.3) years (range: 52–100) vs. 70.0 (26.5) years (range: 28–97 years)). Sepsis and mortality occurred only in the EC group (48.1% and 11.1%, respectively). The univariate analysis of predisposing factors revealed that age, DM, HTN, CVA, CKD, and NB were significantly associated with EC. In the multivariate analysis, DM (OR, 6.251; 95% CI, 2.254–17.250; p < 0.001), CKD (OR, 18.439; 95% CI, 3.421–99.404; p = 0.001), NB (OR, 7.374; 95% CI, 1.993–27.285; p = 0.003) were associated with EC. Conclusions: The results of this study revealed that DM, CKD, and NB were significant risk factors for EC. The tendency toward sepsis and high mortality underscore the need for careful observation while treating patients with EC with the risk noted above.
Uncommon Metastasis of Ovarian Dysgerminoma: A Case Report and Review of the Literature
Authors: Tîrnovanu Mihaela Camelia , Florea Irina Daniela , Tănase Adina , Toma Bogdan Florin , Cojocaru Elena , Ungureanu Carmen , Lozneanu Ludmila
Published: May 2021

Ovarian malignant germ cell tumors (OMGCT) represent less than 10% of all ovarian tumors. Dysgerminoma is the most common malignant primitive germ cell tumor in young women, known for its curability and low propensity to invade and metastasize when diagnosed early. Herein, we report an unusual type of ovarian dysgerminoma (OD) metastasis with a brief review of the literature, lacking similar reported cases. To our knowledge, although there are several case reports of dysgerminoma metastases with variable anatomic location and presentation, vaginal metastasis has not been previously described. The local or systemic relapse together with local and distant metastasis is considered as an independent predictor of poor survival in patients with OD. In light of the absence of mutations status, our patient successfully responded to therapy. Currently, the patient remains in clinical remission. A specific follow-up plan is ongoing knowing that ovarian dysgerminomas tend to recur most often in the first 2–3 years after treatment.
Comparison of Anti-Bacterial Efficacy between Passive Ultrasonic Irrigation and 980 nm-GaAlAs Laser Application in Two Root Types
Authors: Yang Sung-Eun , Kim Yong-Min
Published: May 2021

Background and Objectives: The purpose of the present study was to investigate the anti-bacterial efficacy of passive ultrasonic irrigation (PUI) and a 980-nm-gallium-aluminum-arsenide (GaAlAs) laser using a real-time DNA-based quantitative polymerase chain reaction (qPCR) assay and scanning electron microscopy (SEM). Materials and Methods: Eighty-six extracted single- and double-rooted human teeth were used in the experiment. The following four experimental groups were evaluated, as well as a control group: group 1: single root + PUI (n = 20); group 2: single root + laser application (n = 20); group 3: double roots + PUI (n = 20); group 4: double roots + laser application (n =20); control group (n = 6): 3 single roots, 3 double roots. The qPCR assay was performed in three stages to evaluate the efficacy of the adjunctive method against Enterococcus faecalis. SEM analysis was also used to examine the microstructure of root canal surfaces. The data were statistically analyzed using the Friedman test and the Kruskal–Wallis test with Bonferroni adjustment. Results: The decrease in the DNA levels from S1 (before preparation) to S2 (just after preparation) was highly significant in all groups, and decreases in DNA levels from S2 to S3 (after incubation for 1 week) were found in all experimental groups except group 1. An inter-group analysis showed that PUI was significantly more effective in terms of antibacterial efficacy than GaAlAs laser irradiation in single root (p < 0.05). However, in double roots, experimental groups did not show significantly lower DNA levels than the control group (p > 0.05). SEM images showed that cleaning of the root canal surface and reduction of dentin debris were achieved only in single-rooted teeth when using PUI application. Conclusions: Two adjunctive methods were effective in reducing E. faecalis in single rooted tooth.
Radiation-Grafted Anion-Exchange Membrane for Fuel Cell and Electrolyzer Applications: A Mini Review
Authors: Lim Kean Long , Wong Chun Yik , Wong Wai Yin , Loh Kee Shyuan , Selambakkannu Sarala , Othman Nor Azillah Fatimah , Yang Hsiharng
Published: May 2021

This review discusses the roles of anion exchange membrane (AEM) as a solid-state electrolyte in fuel cell and electrolyzer applications. It highlights the advancement of existing fabrication methods and emphasizes the importance of radiation grafting methods in improving the properties of AEM. The development of AEM has been focused on the improvement of its physicochemical properties, including ionic conductivity, ion exchange capacity, water uptake, swelling ratio, etc., and its thermo-mechano-chemical stability in high-pH and high-temperature conditions. Generally, the AEM radiation grafting processes are considered green synthesis because they are usually performed at room temperature and practically eliminated the use of catalysts and toxic solvents, yet the final products are homogeneous and high quality. The radiation grafting technique is capable of modifying the hydrophilic and hydrophobic domains to control the ionic properties of membrane as well as its water uptake and swelling ratio without scarifying its mechanical properties. Researchers also showed that the chemical stability of AEMs can be improved by grafting spacers onto base polymers. The effects of irradiation dose and dose rate on the performance of AEM were discussed. The long-term stability of membrane in alkaline solutions remains the main challenge to commercial use.
Vibration Characteristics of Hot Rolling Mill Rolls Based on Elastoplastic Hysteretic Deformation
Authors: Peng Rongrong , Zhang Xingzhong , Shi Peiming
Published: May 2021

Based on the analysis of the influence of roll vibration on the elastoplastic deformation state of a workpiece in a rolling process, a dynamic rolling force model with the hysteresis effect is established. Taking the rolling parameters of a 1780 mm hot rolling mill as an example, we analyzed the hysteresis between the dynamic rolling force and the roll vibration displacement by varying the rolling speed, roll radius, entry thickness, front tension, back tension, and strip width. Under the effect of the dynamic rolling force and considering the nonlinear effect between the backup and work rolls as well as the structural constraints on the rolling mill, a hysteretic nonlinear vertical vibration model of a four-high hot rolling mill was established. The amplitude-frequency equations corresponding to 1/2 subharmonic resonance and 1:1 internal resonance of the rolling mill rolls were obtained using a multi-scale approximation method. The amplitude-frequency characteristics of the rolling mill vibration system with different parameters were studied through a numerical simulation. The parametric stiffness and nonlinear stiffness corresponding to the dynamic rolling force were found to have a significant influence on the amplitude of the subharmonic resonance system, the bending degree of the vibration curve, and the size of the resonance region. Moreover, with the change in the parametric stiffness, the internal resonance exhibited an evident jump phenomenon. Finally, the chaotic characteristics of the rolling mill vibration system were studied, and the dynamic behavior of the vibration system was analyzed and verified using a bifurcation diagram, maximum Lyapunov exponent, phase trajectory, and Poincare section. Our research provides a theoretical reference for eliminating and suppressing the chatter in rolling mills subjected to an elastoplastic hysteresis deformation.
Enhanced Microsphere-Assisted Picosecond Laser Processing for Nanohole Fabrication on Silicon via Thin Gold Coating
Authors: Wen Qiuling , Wei Xinyu , Zhang Pengcheng , Lu Jing , Jiang Feng , Lu Xizhao
Published: May 2021

The nanohole arrays on the silicon substrate can effectively enhance the light absorption in thin film silicon solar cells. In order to optimize the solar energy absorption, polystyrene microspheres with diameters of 1 μm are used to assist picosecond laser with a wavelength of 1064 nm to fabricate nanohole arrays on silicon substrate. The experimental results show that the morphology and size of the silicon nanoholes strongly depend on the laser fluence. At 1.19–1.59 J/cm2 laser fluences, well-ordered arrays of nanoholes were fabricated on silicon substrate, with diameters domain from 250 to 549 nm and depths ranging from 60 to 99 nm. However, large amounts of sputtered nanoparticles appeared around the silicon nanoholes. To improve the surface morphology of silicon nanoholes, a nanolayered gold coating is applied on silicon surface to assist laser processing. The results show that, for gold-coated silicon substrate, sputtered nanoparticles around the nanoholes are almost invisible and the cross-sectional profiles of the nanoholes are smoother. Moreover, the ablation rate of the nanoholes on the gold-coated silicon substrate have increased compared to that of the nanoholes on the uncoated one. This simple method allows fast fabrication of well-ordered nanoholes on silicon substrate without sputtered nanoparticles and with smooth inner surface.
Efficacy Estimation of Microbubble-Assisted Local Sonothrombolysis Using a Catheter with a Series of Miniature Transducers
Authors: Li Peiyang , Huang Wenchang , Xu Jie , Shao Weiwei , Cui Yaoyao
Published: May 2021

Intravascular ultrasound has good prospects for clinical applications in sonothrombolysis. The catheter-based side-looking intravascular ultrasound thrombolysis (e.g., Ekosonic catheters) used in clinical studies has a high frequency (2 MHz). The lower-frequency ultrasound requires a larger-diameter transducer. In our study, we designed and manufactured a small ultrasound-based prototype catheter that can emit a lower frequency ultrasound (1.1 MHz). In order to evaluate the safety and efficacy of local low-frequency ultrasound-enhanced thrombolysis, a microbubble (MB) was introduced to augment thrombolysis effect of locally delivered low-intensity ultrasound. The results demonstrated that combination of ultrasound and MB realized higher clot lysis than urokinase-only treatment (17.0% ± 1.2% vs. 14.9% ± 2.7%) under optimal ultrasound settings of 1.1 MHz, 0.414 MPa, 4.89 W/cm2, 5% duty cycle and MB concentration of 60 μg/mL. When urokinase was added, the fibrinolysis accelerated by MB and ultrasound resulted in a further increased thrombolysis rate that was more than two times than that of urokinase alone (36.7% ± 5.5% vs. 14.9% ± 2.7%). However, a great quantity of ultrasound energy was required to achieve substantial clot lysis without MB, leading to the situation that temperature accumulated inside the clot became harmful. We suggest that MB-assisted local sonothrombolysis be considered as adjuvant therapy of thrombolytic agents.
Study of Microwave-Induced Ag Nanowire Welding for Soft Electrode Conductivity Enhancement
Authors: Zhang Meng , Han Songjia , Xuan Zhi-Yang , Fang Xiaohui , Liu Xiaoming , Zhang Wu , Chen Hui-Jiuan
Published: May 2021

Silver nanowire (AgNW)-coated thin films are widely proposed for soft electronics application due to their good conductivity, transparency and flexibility. Here, we studied the microwave welding of AgNW-based soft electrodes for conductivity enhancement. The thermal effect of the microwave to AgNWs was analyzed by dispersing the nanowires in a nonpolar solution, the temperature of which was found to be proportional with the nanowire diameters. AgNWs were then coated on a thin film and welded under microwave heating, which achieved a film conductivity enhancement of as much as 79%. A microwave overheating of AgNWs, however, fused and broke the nanowires, which increased the film resistance significantly. A soft electrode was finally demonstrated using the microwave-welded AgNW thin film, and a 1.13 µA/mM sensitivity was obtained for glucose sensing. Above all, we analyzed the microwave thermal effect on AgNWs to provide a guidance to control the nanowire welding effect, which can be used for film conductivity enhancement and applied for soft and bio-compatible electrodes.
Thermal-Hydrological-Chemical Modeling of a Covered Waste Rock Pile in a Permafrost Region
Authors: Yi Xueying , Su Danyang , Bussière Bruno , Mayer K.
Published: May 2021

In order to reduce contaminant mass loadings, thermal cover systems may be incorporated in the design of waste rock piles located in regions of continuous permafrost. In this study, reactive transport modeling was used to improve the understanding of coupled thermo-hydrological and chemical processes controlling the evolution of a covered waste rock pile located in Northern Canada. Material properties from previous field and laboratory tests were incorporated into the model to constrain the simulations. Good agreement between simulated and observational temperature data indicates that the model is capable of capturing the coupled thermo-hydrological processes occurring within the pile. Simulations were also useful for forecasting the pile’s long-term evolution with an emphasis on water flow and heat transport mechanisms, but also including geochemical weathering processes and sulfate mass loadings as an indicator for the release of contaminated drainage. An uncertainty analysis was carried out to address different scenarios of the cover’s performance as a function of the applied infiltration rate, accounting for the impacts of evaporation, runoff, and snow ablation. The model results indicate that the cover performance is insensitive to the magnitude of recharge rates, except for limited changes of the flow regime in the shallow active layer. The model was expanded by performing an additional sensitivity analysis to assess the role of cover thicknesses. The simulated results reveal that a cover design with an appropriate thickness can effectively minimize mass loadings in drainage by maintaining the active layer completely within the cover.
Study on the Mechanism of Ionic Liquids Improving the Extraction Efficiency of Essential Oil Based on Experimental Optimization and Density Functional Theory: The Fennel (Foeniculi fructus) Essential Oil Case
Authors: Shi Guolin , Lin Longfei , Liu Yuling , Chen Gongsen , Yang Anhui , Wu Yanqiu , Zhou Yingying , Li Hui
Published: May 2021

In this work, microwave-assisted ionic liquids treatment, followed by hydro-distillation (MILT-HD), as an efficient extraction technology, was used to extract essential oil. The purpose for this was to use multivariate analysis (MVA) models to investigate the effects of potential critical process parameters on the extraction efficiency of essential oil, and explore the mechanism of ionic liquids (ILs). According to the design of experiment (DoE), under optimal process conditions, the extraction efficiency of essential oil was dramatically enhanced, and had low energy demands. Since little is known regarding those mechanisms, according to the non-covalent interaction analysis, the underlying mechanism for ILs improving extraction efficiency was explored based on the density functional theory (DFT). The results showed that ILs could form intense non-covalent bond interaction with cellulose. It helped destroy the network hydrogen bond structure of cellulose in plant cells and caused the essential oils in the cells to be more easily exposed to the extraction solution, thereby accelerating extraction efficiency. Based on this work, it is conducive to understand the MILT-HD process better and gain knowledge of the mechanism of ILs.
Impact of Altitudinal Variation on the Phytochemical Profile, Anthelmintic and Antimicrobial Activity of Two Pinus Species
Authors: Elkady Wafaa M. , Gonaid Mariam H. , Yousif Miriam F. , El-Sayed Mahmoud , Omar Hind A. N.
Published: May 2021

Active components from natural sources are the current focus in most pharmacological research to provide new therapeutic agents for clinical use. Essential oils from the Pinus species have been traditionally used in medicine. This study aimed to investigate the chemical profile of two Pinus species, Pinus halepensis L. and Pinus pinea Mill, from different altitudes in Libya and study the effect of environmental conditions on the biological activities of essential oils. A clevenger apparatus was used to prepare the essential oils by hydrodistillation. Analyses were done using GC/MS. Anthelmintic and antimicrobial activities were tested against the earthworm Allolobophora caliginosa, gram-positive bacteria, gram-negative bacteria, and fungi. Different chemical profiles were observed among all tested essential oils, and terpenes were the most dominant class. All studied essential oils from the Pinus species exhibited a remarkable anthelmintic activity compared to the standard piperazine citrate drug. Pinus halepensis from both altitudes showed broad-spectrum antimicrobial activity against all tested microorganisms, while Pinus pinea was effective against only Escherichia coli. From these findings, one can conclude that there are variations between studied species. The essential oil compositions are affected by environmental factors, which consequently affect the anthelmintic and antimicrobial activity.
Modification of Sensory Expression of 3-Isobutyl-2-methoxypyrazine in Wines through Blending Technique
Authors: Ling Mengqi , Zhou Yu , Lan Yibin , Cheng Chifang , Wu Guangfeng , Duan Changqing , Shi Ying
Published: May 2021

Sensory interactions exist between 3-alkyl-2-methoxypyrazines and various volatiles in wines. In this study, the binary blending of Cabernet Franc wines containing high levels of MPs and three monovarietal red wines with two proportions was conducted after fermentation. Volatiles were detected by gas chromatography-mass spectrometry (GC-MS), and wines were evaluated by quantitative descriptive analysis at three-month intervals during six-month bottle aging. Results showed blending wines exhibited lower intensity of ‘green pepper’, especially CFC samples blended by Cabernet Sauvignon wines with an even higher concentration of 3-isobutyl-2-methoxypyrazine (IBMP). Based on Pearson correlation analysis, acetates could promote the expression of ‘tropical fruity’ and suppress ‘green pepper’ caused by IBMP. Positive correlation was observed among ‘green pepper’, ‘herbaceous’, and ‘berry’. The concentration balance between IBMP and other volatiles associated with ‘green pepper’ and fruity notes was further investigated through sensory experiments in aroma reconstitution. Higher pleasant fruity perception was obtained with the concentration proportion of 1-hexanol (1000 μg/L), isoamyl acetate (550 μg/L), ethyl hexanoate (400 μg/L), and ethyl octanoate (900 μg/L) as in CFC samples. Blending wines with proper concentration of those volatiles would be efficient to weaken ‘green pepper’ and highlight fruity notes, which provided scientific theory on sensory modification of IBMP through blending technique.
Potential Chemicals from Plastic Wastes
Authors: Prajapati Ravindra , Kohli Kirtika , Maity Samir , Sharma Brajendra
Published: May 2021

Plastic is referred to as a “material of every application”. From the packaging and automotive industries to the medical apparatus and computer electronics sectors, plastic materials are fulfilling demands efficiently. These plastics usually end up in landfills and incinerators, creating plastic waste pollution. According to the Environmental Protection Agency (EPA), in 2015, 9.1% of the plastic materials generated in the U.S. municipal solid waste stream was recycled, 15.5% was combusted for energy, and 75.4% was sent to landfills. If we can produce high-value chemicals from plastic wastes, a range of various product portfolios can be created. This will help to transform chemical industries, especially the petrochemical and plastic sectors. In turn, we can manage plastic waste pollution, reduce the consumption of virgin petroleum, and protect human health and the environment. This review provides a description of chemicals that can be produced from different plastic wastes and the research challenges involved in plastic waste to chemical production. This review also provides a brief overview of the state-of-the-art processes to help future system designers in the plastic waste to chemicals area.
Temperature Controlled Loading and Release of the Anti-Inflammatory Drug Cannabidiol by Smart Microgels
Authors: Dirksen Maxim , Kinder Timo Alexander , Brändel Timo , Hellweg Thomas
Published: May 2021

CBD is a promising candidate for treatment of many diseases and plays a major role in the growing trend to produce high-end drugs from natural, renewable resources. In the present work, we demonstrate a way to incorporate the anti-inflammatory drug CBD into smart microgel particles. The copolymer microgels that we chose as carrier systems exhibit a volume phase transition temperature of 39 ∘C, which is just above normal body temperature and makes them ideal candidates for hyperthermia treatment. While a simple loading route of CBD was not successful due to the enormous hydrophobicity of CBD, an alternative route was developed by immersing the microgels in ethanol. Despite the expected loss of thermoresponsive behaviour of the microgel matrix due to the solvent exchange, a temperature-dependent release of CBD was detected by the material, creating an interesting question of interactions between CBD and the microgel particles in ethanol. Furthermore, the method developed for loading of the microgel particles with CBD in ethanol was further improved by a subsequent transfer of the loaded particles into water, which proves to be an even more promising approach due to the successful temperature-dependent release of the drug above the collapse temperature of the microgels.