bluuebunny/crossref_metadata_2025 huggingface dataset:
Cynandione A Alleviates Neuropathic Pain Through α7-nAChR-Dependent IL-10/β-Endorphin Signaling Complexes
Authors: Han Qiao-Qiao , Yin Min , Wang Zi-Ying , Liu Hao , Ao Jun-Ping , Wang Yong-Xiang
Published: January 2021

Cynandione A, an acetophenone isolated from Cynanchum Wilfordii Radix, exhibits antineuropathic pain effect. This study further explored the target molecule and signaling mechanisms underlying cynandione-A-induced antineuropathic pain. Intrathecal injection of cynandione A significantly attenuated mechanical allodynia in neuropathic rats and substantially increased spinal expression of IL-10 and β-endorphin but not dynorphin A. Cynandione A treatment also enhanced expression of IL-10 and β-endorphin but not α7 nicotinic acetylcholine receptors (nAChRs) in cultured microglia. The IL-10 antibody attenuated cynandione-A-induced spinal or microglial gene expression of β-endorphin and mechanical allodynia, whereas the β-endorphin antiserum blocked cynandione-A-induced mechanical antiallodynia but not spinal or microglial IL-10 gene expression. The α7 nAChR antagonist methyllycaconitine significantly reduced cynandione-A-induced mechanical antiallodynia and spinal or microglial expression of IL-10 and β-endorphin. Furthermore, cynandione A stimulated microglial phosphorylation of PKA, p38, and CREB in an α7-nAChR-dependent manner, and treatment with their inhibitors attenuated cynandione-A-induced mechanical antiallodynia and spinal or microglial expression of IL-10 and β-endorphin. In addition, cynandione A stimulated spinal phosphorylation of the transcription factor STAT3, which was inhibited by methyllycaconitine, the PKA activation inhibitor or IL-10 antibody. The STAT3 inhibitor NSC74859 also abolished cynandione-A-induced mechanical antiallodynia and spinal expression of β-endorphin. These findings suggest that cynandione A suppresses neuropathic pain through α7-nAChR-dependent IL-10/β-endorphin signaling pathway in spinal microglia.
Challenges for Adolescents With Congenital Heart Defects/Chronic Rheumatic Heart Disease and What They Need: Perspectives From Patients, Parents and Health Care Providers at the Institut Jantung Negara (National Heart Institute), Malaysia
Authors: Tye Sue Kiat , Kandavello Geetha , Wan Ahmadul Badwi Syarifah Azizah , Abdul Majid Hariyati Sharima
Published: January 2021

Objectives

This study aimed to describe the experiences and challenges faced by adolescents with moderate and severe congenital heart defects (CHD) or Chronic Rheumatic Heart Disease (CRHD) and to determine their needs in order to develop an Adolescent Transition Psychoeducational Program.

Methods

The study involved seven adolescents with moderate to severe CHD/CRHD, six parents, and four health care providers in Institute Jantung Negara (National Heart Institute). Participants were invited for a semi-structured interview. Qualitative data were analyzed through the Atlas.ti 7 program using triangulation methods.

Results/conclusions

We identified five themes concerning the experience and challenges of adolescents relating to: (1) emotional/psychological issues; (2) the progress of the illness; (3) relationship issues; (4) future preparation; and, (5) school and community. These themes were identified together with eleven subcategories. The staff expressed support for the development of the Adolescent Transition Psychoeducational Program and adolescents with CHD/CRHD and their parents were willing to participate in the program if their schedule allowed. Their suggestions to improve the program were classified into six categories, with two main themes, (1) the self-management of illness in life and the future; and, (2) social support. In conclusion, the findings from the situation analysis act as a basis for a conceptual framework that will contribute to the development of an Adolescent Transition Psychoeducational Program that aims to empower adolescents with CHD/CRHD, enabling them to manage challenges during the transition phase between childhood and adulthood.
Planting the Seeds: Orchestral Music Education as a Context for Fostering Growth Mindsets
Authors: Holochwost Steven J. , Bose Judith Hill , Stuk Elizabeth , Brown Eleanor D. , Anderson Kate E. , Wolf Dennie Palmer
Published: January 2021

Growth mindset is an important aspect of children’s socioemotional development and is subject to change due to environmental influence. Orchestral music education may function as a fertile context in which to promote growth mindset; however, this education is not widely available to children facing economic hardship. This study examined whether participation in a program of orchestral music education was associated with higher levels of overall growth mindset and greater change in levels of musical growth mindset among children placed at risk by poverty. After at least 2 years of orchestral participation, students reported significantly higher levels of overall growth mindset than their peers; participating students also reported statistically significant increases in musical growth mindset regardless of the number of years that they were enrolled in orchestral music education. These findings have implications for future research into specific pedagogical practices that may promote growth mindset in the context of orchestral music education and more generally for future studies of the extra-musical benefits of high-quality music education.
Factor Structure and Validation of the Undergraduate Teaching Faculty Investment Questionnaire
Authors: Sun Lizhi , Zhang Hong , Xu Jingjing
Published: January 2021

Undergraduate education is very important for higher education. Among the factors affecting the quality of undergraduate education, teaching quality is the most important factor. At present, an important issue that affects teaching quality is insufficient teaching investment. An open-ended questionnaire survey was conducted on 62 faculty members and 65 university students in China. Results found that the undergraduate teaching investment of faculty members mainly consists of four parts: workload investment, ability investment, energy investment, and teaching emotional investment. A preliminary test was conducted on 342 faculty members, and the results of an exploratory factor analysis supported the proposed four-dimensional teaching investment model. The test involved 293 faculty members and confirmatory factor analysis showed that the four-dimensional model had a good degree of fit. A further criterion-related test showed that the four-dimensional teaching investment of faculty members has a significant positive correlation with classroom management, clarity of teaching materials, teacher–student interaction, teaching strategies, and skills application, and work engagement. These results show that the proposed four dimensions of teaching investment effectively measure the teaching investment of faculty members, specifically the indicators of teaching investment and that they promote the development of faculty teaching investment research.
Evidence-Based Medicine and the Potential for Inclusion of Non-Biomedical Health Systems: The Case for Taijiquan
Authors: Langweiler Mark J.
Published: January 2021

Many traditional and complementary and alternative healthcare systems or practices, such as Traditional Chinese Medicine, taijiquan, or acupuncture, are easily found in many North American and European cities. For the most part these practices are not accredited, and their validation remains limited. This is primarily the result of the lack of modern scientific research. Additionally, the studies that are performed rely on evidence and research designs that often negate the true features of these practices with a loss of authenticity. Is it possible or even desirable for these systems to acquire accreditation and inclusion? If so, given the apparent, subjective nature of these practices, can a pluralistic approach to healthcare that retains the Western values of science and medicine be developed that yet respects the diversity of different concepts about life, health and services while permitting these practices to maintain their authenticity? And is it possible to develop a regulatory framework that practitioners can use? The current paper examines questions concerning the uses of non-Western healthcare practices without the loss of their authentic nature. The process of integration is here examined using the inclusion of taijiquan as a health-promoting martial art as the model.
Hamiltonian Renormalization V: Free Vector Bosons
Authors: Liegener K. , Thiemann T.
Published: January 2021

In a recent proposal we applied methods from constructive QFT to derive a Hamiltonian Renormalization Group in order to employ it ultimately for canonical quantum gravity. The proposal was successfully tested for free scalar fields and thus a natural next step is to test it for free gauge theories. This can be done in the framework of reduced phase space quantization which allows using techniques developed earlier for scalar field theories. In addition, in canonical quantum gravity one works in representations that support holonomy operators which are ill defined in the Fock representation of say Maxwell or Proca theory. Thus, we consider toy models that have both features, i.e. which employ Fock representations in which holonomy operators are well-defined. We adapt the coarse graining maps considered for scalar fields to those theories for free vector bosons. It turns out that the corresponding fixed pointed theories can be found analytically.
How Does Citizen Science Compare to Online Survey Panels? A Comparison of Food Knowledge and Perceptions Between the Zooniverse, Prolific and Qualtrics UK Panels
Authors: Armstrong Beth , Reynolds Christian , Bridge Gemma , Oakden Libby , Wang Changqiong , Panzone Luca , Schmidt Rivera Ximena , Kause Astrid , Ffoulkes Charles , Krawczyk Coleman , Miller Grant , Serjeant Stephen
Published: January 2021

With an increasing focus on the uptake of healthy and sustainable diets, a growing body of research has explored consumer perceptions and understanding of the environmental impacts and safety of foods. However, this body of research has used a wide range of methods to recruit participants, which can influence the results obtained. The current research explores the impact of different recruitment methods upon observed estimations of the carbon footprint (gCO2e), energy content (Kcal), food safety and animal using three different online recruitment platforms; Qualtrics (N= 397), Prolific (N= 407), Zooniverse (N~601, based on unique IP addresses). Qualtrics and Prolific participants rated the carbon footprint, energy content, food safety and animal welfare of all foods in the survey. Zooniverse citizens rated the carbon footprint or energy content then food safety or animal welfare of all foods in the survey. Kruskal-Wallis and Chi-square analyses compared the energy content and carbon footprint estimations with validated values, and differences in estimate accuracy and perceptions between recruitment methods. Participants were unable to accurately estimate the carbon footprint and energy content of foods. The carbon footprint of all foods were overestimated, with the exception of beef and lamb which was underestimated. The calorie content of fruits and vegetables are typically overestimated. Perceptions of animal welfare and food safety differed by recruitment method. Zooniverse citizens rated animal welfare standards to be lower for meat products and eggs, compared to Qualtrics and Prolific participants. Overall, Qualtrics participants typically held the highest food risk perceptions, however this varied by food type. The lack of knowledge about the carbon footprint and energy content of foods demonstrates the need for consumer education and communication to enable the move toward healthier and more sustainable diets. Perceptions of food safety and animal welfare demonstrate a baseline from which to develop consumer focused communications and governance. We have shown that different recruitment tools can result in differences in observed perceptions. This highlights the need to carefully consider the recruitment tool being used in research when assessing participant knowledge and perceptions.
Genomic Analysis of an Indian G8P[1] Caprine Rotavirus-A Strain Revealing Artiodactyl and DS-1-Like Human Multispecies Reassortment
Authors: Sircar Shubhankar , Malik Yashpal Singh , Kumar Prashant , Ansari Mohd Ikram , Bhat Sudipta , Shanmuganathan S. , Kattoor Jobin Jose , Vinodhkumar O.R. , Rishi Narayan , Touil Nadia , Ghosh Souvik , Bányai Krisztián , Dhama Kuldeep
Published: January 2021

The surveillance studies for the presence of caprine rotavirus A (RVA) are limited in India, and the data for the whole-genome analysis of the caprine RVA is not available. This study describes the whole-genome-based analysis of a caprine rotavirus A strain, RVA/Goat-wt/IND/K-98/2015, from a goat kid in India. The genomic analysis revealed that the caprine RVA strain K-98, possess artiodactyl-like and DS-1 human-like genome constellation G8P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The three structural genes (VP2, VP4, and VP7) were close to caprine host having nucleotide-based identity range between 97.5 and 98.9%. Apart from them, other gene segments showed similarity with either bovine or human like genes, ultimately pointing toward a common evolutionary origin having an artiodactyl-type backbone of strain K-98. Phylogenetically, the various genes of the current study isolate also clustered inside clades comprising Human-Bovine-Caprine isolates from worldwide. The current findings add to the knowledge on caprine rotaviruses and might play a substantial role in designing future vaccines or different alternative strategies combating such infections having public health significance. To the best of our knowledge, this is the first report on the whole-genome characterization of a caprine RVA G8P[1] strain from India. Concerning the complex nature of the K-98 genome, whole-genome analyses of more numbers of RVA strains from different parts of the country are needed to comprehend the genomic nature and genetic diversity among caprine RVA.
Computed Tomography Analysis of the Feline Infraorbital Foramen and Canal
Authors: Davis Lily V. , Hoyer Naomi K. , Boscan Pedro , Rao Sangeeta , Rawlinson Jennifer E.
Published: January 2021

Feline skull anatomic variation is plain to see with casual observation. Obtaining an in-depth understanding of this anatomic variability is critical to performing safe and effective regional anesthesia for dental procedures and maxillofacial surgeries. Maxillofacial anatomic variability is proven to impact the placement and efficacy of nerve blocks in dogs and horses, but similar studies have not been performed in cats. This study's main objective was to evaluate the anatomy of the infraorbital foramen and canal in relation to regional anatomic landmarks in brachycephalic and mesaticephalic cats. Significant anatomic variability was identified, particularly among cats with brachycephalic skulls.
Acknowledgment to Reviewers of Atoms in 2020
Authors:   Atoms Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Atoms maintains its standards for the high quality of its published papers [...]
Hyperpolarized 13C Spectroscopy with Simple Slice-and-Frequency-Selective Excitation
Authors: Topping Geoffrey J. , Heid Irina , Trajkovic-Arsic Marija , Kritzner Lukas , Grashei Martin , Hundshammer Christian , Aigner Maximilian , Skinner Jason G. , Braren Rickmer , Schilling Franz
Published: January 2021

Hyperpolarized 13C nuclear magnetic resonance spectroscopy can characterize in vivo tissue metabolism, including preclinical models of cancer and inflammatory disease. Broad bandwidth radiofrequency excitation is often paired with free induction decay readout for spectral separation, but quantification of low-signal downstream metabolites using this method can be impeded by spectral peak overlap or when frequency separation of the detected peaks exceeds the excitation bandwidth. In this work, alternating frequency narrow bandwidth (250 Hz) slice-selective excitation was used for 13C spectroscopy at 7 T in a subcutaneous xenograft rat model of human pancreatic cancer (PSN1) to improve quantification while measuring the dynamics of injected hyperpolarized [1-13C]lactate and its metabolite [1-13C]pyruvate. This method does not require sophisticated pulse sequences or specialized radiofrequency and gradient pulses, but rather uses nominally spatially offset slices to produce alternating frequency excitation with simpler slice-selective radiofrequency pulses. Additionally, point-resolved spectroscopy was used to calibrate the 13C frequency from the thermal proton signal in the target region. This excitation scheme isolates the small [1-13C]pyruvate peak from the similar-magnitude tail of the much larger injected [1-13C]lactate peak, facilitates quantification of the [1-13C]pyruvate signal, simplifies data processing, and could be employed for other substrates and preclinical models.
Risk Assessment and Antithrombotic Strategies in Antiphospholipid Antibody Carriers
Authors: Calcaterra Ilenia , Ambrosino Pasquale , Vitelli Nicoletta , Lupoli Roberta , Orsini Roberta Clara , Chiurazzi Martina , Maniscalco Mauro , Di Minno Matteo Nicola Dario
Published: January 2021

Antiphospholipid antibodies (aPL) are a cluster of autoantibodies directed against plasma proteins with affinity for membrane phospholipids. The most frequently tested aPL are lupus anticoagulant (LA), anti-cardiolipin antibodies (aCL), and anti-β2-glycoprotein I antibodies (anti-β2GPI). aPL play a key pathogenic role in the development of the antiphospholipid syndrome (APS), a systemic autoimmune disease characterized by recurrent thrombotic and/or pregnancy complications in patients with persistent aPL. However, aPL positivity is occasionally documented in patients with no previous history of thrombotic or pregnancy morbidity. LA activity, multiple aPL positivity, high-titer aPL, and a concomitant systemic autoimmune disease are recognized risk factors for future thrombotic events in asymptomatic carriers. Moreover, an accelerated atherosclerosis with increased cardiovascular (CV) risk has also been associated with aPL positivity, thus exposing aPL carriers to fatal complications and chronic disability requiring cardiac rehabilitation. Overall, an accurate risk stratification is recommended for aPL-positive subjects in order to prevent both venous and arterial thrombotic complications. In this review, we provide an overview of the main antithrombotic and risk assessment strategies in aPL carriers.
Magnesium, Little Known But Possibly Relevant: A Link between NASH and Related Comorbidities
Authors: Simón Jorge , Delgado Teresa Cardoso , Martinez-Cruz Luis Alfonso , Martínez-Chantar Maria Luz
Published: January 2021

Non-alcoholic steatohepatitis (NASH) is characterized by an abnormal hepatic lipid accumulation accompanied by a necro-inflammatory process and a fibrotic response. It comprises from 10% to 30% of cases of patients with non-alcoholic liver disease, which is a global health problem affecting around a quarter of the worldwide population. Nevertheless, the development of NASH is often surrounded by a pathological context with other comorbidities, such as cardiovascular diseases, obesity, insulin resistance or type 2 diabetes mellitus. Dietary imbalances are increasingly recognized as the root cause of these NASH-related comorbidities. In this context, a growing concern exists about whether magnesium consumption in the general population is sufficient. Hypomagnesemia is a hallmark of the aforementioned NASH comorbidities, and deficiencies in magnesium are also widely related to the triggering of complications that aggravate NASH or derived pathologies. Moreover, the supplementation of this cation has proved to reduce mortality from hepatic complications. In the present review, the role of magnesium in NASH and related comorbidities has been characterized, unraveling the relevance of maintaining the homeostasis of this cation for the correct functioning of the organism.
Biological Evaluation of Oil-in-Water Microemulsions as Carriers of Benzothiophene Analogues for Dermal Applications
Authors: Theochari Ioanna , Ilic Tanja , Nicolic Ines , Dobricic Vladimir , Tenchiou Alia , Papahatjis Demetris , Savic Snezana , Xenakis Aristotelis , Papadimitriou Vassiliki , Pletsa Vasiliki
Published: January 2021

During the last decade, many studies have been reported on the design and formulation of novel drug delivery systems proposed for dermal or transdermal administration. The efforts focus on the development of biocompatible nanodispersions that can be delivered to the skin and treat severe skin disorders, including cancer. In this context, oil-in-water (O/W) microemulsions have been developed to encapsulate and deliver lipophilic bioactive molecules for dermal application. An O/W biocompatible microemulsion composed of PBS buffer, Tween 80, and triacetin was assessed for its efficacy as a drug carrier of DPS-2, a lead compound, initially designed in-house to inhibit BRAFV600E oncogenic kinase. The system was evaluated through both in vitro and ex vivo approaches. The cytotoxic effect, in the presence and absence of DPS-2, was examined through the thiazolyl blue tetrazolium bromide (MTT) cell proliferation assay using various cell lines. Further investigation through Western blotting revealed that cells died of necrosis. Porcine ear skin was used as a skin model to evaluate the degree of permeation of DPS-2 through skin and assess its retention. Through the ex vivo experiments, it was clarified that encapsulated DPS-2 was distributed within the full thickness of the stratum corneum (SC) and had a high affinity to hair follicles.
Correction: Ahn, J.W., et al. CeRNA Network Analysis Representing Characteristics of Different Tumor Environments Based on 1p/19q Codeletion in Oligodendrogliomas. Cancers 2020, 12, 2543
Authors: Ahn Ju Won , Park YoungJoon , Kang Su Jung , Hwang So Jung , Cho Kyung Gi , Lim JaeJoon , Kwack KyuBum
Published: January 2021

The authors wish to make the following corrections to this paper [...]
Acknowledgment to Reviewers of Cells in 2020
Authors:   Cells Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Cells maintains its standards for the high quality of its published papers [...]
A Perfect Match: Converging and Automating Privacy and Security Impact Assessment On-the-Fly
Authors: Papamartzivanos Dimitrios , Menesidou Sofia Anna , Gouvas Panagiotis , Giannetsos Thanassis
Published: January 2021

As the upsurge of information and communication technologies has become the foundation of all modern application domains, fueled by the unprecedented amount of data being processed and exchanged, besides security concerns, there are also pressing privacy considerations that come into play. Compounding this issue, there is currently a documented gap between the cybersecurity and privacy risk assessment (RA) avenues, which are treated as distinct management processes and capitalise on rather rigid and make-like approaches. In this paper, we aim to combine the best of both worlds by proposing the APSIA (Automated Privacy and Security Impact Assessment) methodology, which stands for Automated Privacy and Security Impact Assessment. APSIA is powered by the use of interdependency graph models and data processing flows used to create a digital reflection of the cyber-physical environment of an organisation. Along with this model, we present a novel and extensible privacy risk scoring system for quantifying the privacy impact triggered by the identified vulnerabilities of the ICT infrastructure of an organisation. We provide a prototype implementation and demonstrate its applicability and efficacy through a specific case study in the context of a heavily regulated sector (i.e., assistive healthcare domain) where strict security and privacy considerations are not only expected but mandated so as to better showcase the beneficial characteristics of APSIA. Our approach can complement any existing security-based RA tool and provide the means to conduct an enhanced, dynamic and generic assessment as an integral part of an iterative and unified risk assessment process on-the-fly. Based on our findings, we posit open issues and challenges, and discuss possible ways to address them, so that such holistic security and privacy mechanisms can reach their full potential towards solving this conundrum.
Language Bias in the Google Scholar Ranking Algorithm
Authors: Rovira Cristòfol , Codina Lluís , Lopezosa Carlos
Published: January 2021

The visibility of academic articles or conference papers depends on their being easily found in academic search engines, above all in Google Scholar. To enhance this visibility, search engine optimization (SEO) has been applied in recent years to academic search engines in order to optimize documents and, thereby, ensure they are better ranked in search pages (i.e., academic search engine optimization or ASEO). To achieve this degree of optimization, we first need to further our understanding of Google Scholar’s relevance ranking algorithm, so that, based on this knowledge, we can highlight or improve those characteristics that academic documents already present and which are taken into account by the algorithm. This study seeks to advance our knowledge in this line of research by determining whether the language in which a document is published is a positioning factor in the Google Scholar relevance ranking algorithm. Here, we employ a reverse engineering research methodology based on a statistical analysis that uses Spearman’s correlation coefficient. The results obtained point to a bias in multilingual searches conducted in Google Scholar with documents published in languages other than in English being systematically relegated to positions that make them virtually invisible. This finding has important repercussions, both for conducting searches and for optimizing positioning in Google Scholar, being especially critical for articles on subjects that are expressed in the same way in English and other languages, the case, for example, of trademarks, chemical compounds, industrial products, acronyms, drugs, diseases, etc.
Fluid Mechanics of Plankton
Authors: Yamazaki Hidekatsu , Strickler J. Rudi
Published: January 2021

These first lines of Hensen’s article (Figure 1) in the “Fünfter Bericht” (1887) translate as follows [...]
Acknowledgment to Reviewers of Games in 2020
Authors:   Games Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Games maintains its standards for the high quality of its published papers [...]
The Axenfeld–Rieger Syndrome Gene FOXC1 Contributes to Left–Right Patterning
Authors: Chrystal Paul W. , French Curtis R. , Jean Francesca , Havrylov Serhiy , van Baarle Suey , Peturson Ann-Marie , Xu Pengfei , Crump J. Gage , Pilgrim David B. , Lehmann Ordan J. , Waskiewicz Andrew J.
Published: January 2021

Precise spatiotemporal expression of the Nodal-Lefty-Pitx2 cascade in the lateral plate mesoderm establishes the left–right axis, which provides vital cues for correct organ formation and function. Mutations of one cascade constituent PITX2 and, separately, the Forkhead transcription factor FOXC1 independently cause a multi-system disorder known as Axenfeld–Rieger syndrome (ARS). Since cardiac involvement is an established ARS phenotype and because disrupted left–right patterning can cause congenital heart defects, we investigated in zebrafish whether foxc1 contributes to organ laterality or situs. We demonstrate that CRISPR/Cas9-generated foxc1a and foxc1b mutants exhibit abnormal cardiac looping and that the prevalence of cardiac situs defects is increased in foxc1a−/−; foxc1b−/− homozygotes. Similarly, double homozygotes exhibit isomerism of the liver and pancreas, which are key features of abnormal gut situs. Placement of the asymmetric visceral organs relative to the midline was also perturbed by mRNA overexpression of foxc1a and foxc1b. In addition, an analysis of the left–right patterning components, identified in the lateral plate mesoderm of foxc1 mutants, reduced or abolished the expression of the NODAL antagonist lefty2. Together, these data reveal a novel contribution from foxc1 to left–right patterning, demonstrating that this role is sensitive to foxc1 gene dosage, and provide a plausible mechanism for the incidence of congenital heart defects in Axenfeld–Rieger syndrome patients.
Predictive Value of Circulating miRNAs in Lymph Node Metastasis for Colon Cancer
Authors: Lee In Hee , Kim Gyeonghwa , Kwak Sang Gyu , Baek Dong Won , Kang Byung Woog , Kim Hye Jin , Park Su yeon , Park Jun Seok , Choi Gyu-Seog , Hur Keun , Kim Jong Gwang
Published: January 2021

(1) Background: Lymph node (LN) status is an indubitable prognostic factor for survival among colon cancer patients. MicroRNAs (miRNAs) have been implicated in the development and progression of many cancers and are potential biomarkers for cancer diagnosis and prognosis. Therefore, we validated candidate biomarkers using circulating miRNAs by analyzing the plasma miRNA concentrations from patients with colon cancer to predict LN metastasis. (2) Methods: This study included 79 blood samples from patients diagnosed with colon cancer. The NanoString assay was used for screening, and TaqMan miRNA assays for quantitative real-time polymerase chain reaction (RT-PCR) test was used for validation. In a discovery set, we compared the expression of 800 circulating miRNAs in 24 samples (stage 0/I/IIA versus IIIB/IIIC). For validation, a total 79 samples were tested using quantitative RT-PCR. (3) Results: In the discovery set, 10 candidate circulating miRNAs were detected (4 up-regulated miRNAs: miR-323a-3p, miR-382-5p, miR-29a-3p, and miR-376a-3p; 6 down-regulated miRNAs: miR-26a-5p, let-7g-5p, miR-15b-5p, miR-142-3p, miR-374a-5p, and let-7b-5p). In the validation set, higher expression of three circulating miRNAs (miR-323a-3p, miR-382-5p, and miR-376a-3p) was significantly associated with LN metastasis (p = 0.0063, 0.0107, and 0.0022). (4) Conclusions: High expression of circulating miR-323a-3p, miR-382-5p, and miR-376a-3p was significantly associated with LN metastasis in colon cancer patients. These miRNAs could be circulating biomarker candidates that predict the presence of LN metastasis.
The Tomato Interspecific NB-LRR Gene Arsenal and Its Impact on Breeding Strategies
Authors: Andolfo Giuseppe , D’Agostino Nunzio , Frusciante Luigi , Ercolano Maria Raffaella
Published: January 2021

Tomato (Solanum lycopersicum L.) is a model system for studying the molecular basis of resistance in plants. The investigation of evolutionary dynamics of tomato resistance (R)-loci provides unique opportunities for identifying factors that promote or constrain genome evolution. Nucleotide-binding domain and leucine-rich repeat (NB-LRR) receptors belong to one of the most plastic and diversified families. The vast amount of genomic data available for Solanaceae and wild tomato relatives provides unprecedented insights into the patterns and mechanisms of evolution of NB-LRR genes. Comparative analysis remarked a reshuffling of R-islands on chromosomes and a high degree of adaptive diversification in key R-loci induced by species-specific pathogen pressure. Unveiling NB-LRR natural variation in tomato and in other Solanaceae species offers the opportunity to effectively exploit genetic diversity in genomic-driven breeding programs with the aim of identifying and introducing new resistances in tomato cultivars. Within this motivating context, we reviewed the repertoire of NB-LRR genes available for tomato improvement with a special focus on signatures of adaptive processes. This issue is still relevant and not thoroughly investigated. We believe that the discovery of mechanisms involved in the generation of a gene with new resistance functions will bring great benefits to future breeding strategies.
Access to Medicines via Non-Pharmacy Outlets in European Countries—A Review of Regulations and the Influence on the Self-Medication Phenomenon
Authors: Oleszkiewicz Patrycja , Krysinski Jerzy , Religioni Urszula , Merks Piotr
Published: January 2021

Non-pharmacy trade concerns the sale of medicinal products outside of pharmacies, such as limited-service pharmacies, supermarkets, petrol stations, shops open to the public, and kiosks. Access to medicinal products via non-pharmacy outlets varies across the European countries, with a general deregulation of this market area observable. Increasing the availability of medicines by allowing patients to obtain them outside of pharmacies contributes to the spread of self-medication. The aim of this article was to review the legal regulations enabling the non-pharmacy trade in OTC (over the counter) medicinal products in European countries, with particular emphasis on the analysis of active substances contained in medicines available in the non-pharmacy trade. This analysis has made it possible to distinguish three categories of countries: (1) where there is a non-pharmacy trade in OTC medicinal products, (2) where there is a limited non-pharmacy trade in OTC medicines, (3) where there is only a pharmacy trade in OTC medicinal products. In the context of these considerations, we highlight the impact of patient access to medicinal products via non-pharmacy sources on raising the prevalence of self-medication. This article identifies the advantages and risks of self-medication, emphasising the role of the pharmacist as an advisor to patients within the scope of the therapies used.
Aqu@teach—The First Aquaponics Curriculum to Be Developed Specifically for University Students
Authors: Milliken Sarah , Ovca Andrej , Antenen Nadine , Villarroel Morris , Bulc Tjaša , Kotzen Benz , Junge Ranka
Published: January 2021

Aquaponic food production requires a broad spectrum of knowledge in order to understand and manage the processes involved, and for commercial aquaponics to develop its full potential, it will require an appropriately trained workforce. Devised in collaboration as an Erasmus+ Strategic Partnership for Higher Education, Aqu@teach covers the basics of aquaponics with a focus on transferable and entrepreneurial skills. The aquaponics curriculum can either be taught using blended learning—combining digital media and the internet with classroom formats that require the physical co-presence of the teacher and students—or as an e-learning course. The supplementary entrepreneurial skills module was devised on the basis of two surveys: of aquaponics companies around the world, in order to get a broad overview of the skills that are important in the early years of a business; and of European higher education institutions that teach subjects where aquaponics could be incorporated as an optional module. The entrepreneurial skills curriculum introduces the main processes involved in developing a business idea into a start-up company. All of the Aqu@teach resources—the e-learning modules, textbooks, module guides for students, curriculum guides for teachers, best practice guide for teaching aquaponics, and toolbox of innovative didactic practices—are open access.
Acknowledgment to Reviewers of Hydrology in 2020
Authors:   Hydrology Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Hydrology maintains its standards for the high quality of its published papers [...]
Reflections on the Importance of Cost of Illness Analysis in Rare Diseases: A Proposal
Authors: Armeni Patrizio , Cavazza Marianna , Xoxi Entela , Taruscio Domenica , Kodra Yllka
Published: January 2021

In the field of rare diseases (RDs), the evidence standard is often lower than that required by health technology assessment (HTA) and payer authorities. In this commentary, we propose that appropriate economic evaluation for rare disease treatments should be initially informed by cost-of-illness (COI) studies conducted using a societal perspective. Such an approach contributes to improving countries’ understanding of RDs in their entirety as societal and not merely clinical, or product-specific issues. In order to exemplify how the disease burden’s distribution has changed over the last fifteen years, key COI studies for Hemophilia, Fragile X Syndrome, Cystic Fibrosis, and Juvenile Idiopathic Arthritis are examined. Evidence shows that, besides methodological variability and cross-country differences, the disease burden’s share represented by direct costs generally grows over time as novel treatments become available. Hence, to support effective decision-making processes, it seems necessary to assess the re-allocation of the burden produced by new medicinal products, and this approach requires identifying cost drivers through COI studies with robust design and standardized methodology.
Effects of Acute Visual Stimulation Exercise on Attention Processes: An ERP Study
Authors: Wu Shanshan , Ji Hongqing , Won Junyeon , Liu Xiaolong , Park Jung-Jun
Published: January 2021

Backgrounds: It remains to be determined whether visual stimuli during exercise differentially influence the attention process. The purpose of the present study was to examine if different color stimuli during aerobic exercise are associated with different attention processes. Methods: 22 college students completed a four 30-min running session during the presentation of different color stimuli (blue, green, red, and yellow) and without color stimulus on separate visits. The Kanizsa triangle task was administrated before and immediately after exercise to assess the attention process. Behavioral performance (accuracy and response time (RT)) and event-related potential (P2, N2b and P3a) were recorded during the test. Results: Valid/invalid cue RT during the Kaniza test performance was significantly faster following the presentation of color stimuli during treadmill exercise compared to the seated rest. During exercise, these changes were larger after green and yellow stimuli than red in invalid cue RT. P2, N2b and P3a amplitudes of green were significantly larger than the other colors for both valid and invalid cues. Red color showed the lowest P2 and P3a amplitudes for both valid and invalid cues among colors. Conclusion: The distinctive neurocognitive changes during aerobic exercise suggest different effects of color stimuli on visual search attention, attention capture, attentional orienting and processing speed. This study will be a first step to understand the optimal environmental setting during exercise for subsequent improvements in the attention process.
Effect of Simulated Gastrointestinal Tract Conditions on Survivability of Probiotic Bacteria Present in Commercial Preparations
Authors: Stasiak-Różańska Lidia , Berthold-Pluta Anna , Pluta Antoni Stanisław , Dasiewicz Krzysztof , Garbowska Monika
Published: January 2021

Probiotics are recommended, among others, in the diet of children who are under antibiotic therapy, or that suffer from food allergies or travel diarrhea, etc. In the case of toddlers taking probiotic preparations, it is highly recommended to first remove the special capsule, which normally protects probiotic strains against hard conditions in the gastrointestinal tract. Otherwise, the toddler may choke. This removal can impair probiotic survival and reduce its efficacy in a toddler’s organism. The aim of this study was to evaluate the survivability of five strains of lactic acid bacteria from the commercial probiotics available on the Polish market under simulated conditions of the gastrointestinal tract. Five probiotics (each including one of these strains: Bifidobacterium BB-12, Lactobacillus (Lb.) rhamnosus GG, Lb. casei, Lb. acidophilus, Lb. plantarum) were protective capsule deprived, added in a food matrix (chicken–vegetable soup) and subjected under simulated conditions of the gastric and gastrointestinal passage. Strain survivability and possibility to growth were evaluated. Obtained results showed that, among all analyzed commercial probiotic strains, the Lb. plantarum was the most resistant to the applied conditions of the culture medium. They showed a noticeable growth under both in vitro gastric conditions at pH 4.0 and 5.0, as well as in vitro intestinal conditions at all tested concentrations of bile salts.
Virtual Home Assistant Use and Perceptions of Usefulness by Older Adults and Support Person Dyads
Authors: F. Corbett Cynthia , M. Combs Elizabeth , J. Wright Pamela , L. Owens Otis , Stringfellow Isabel , Nguyen Thien , Van Son Catherine R.
Published: January 2021

Aim: Describe virtual home assistant use and usefulness from the perspective of older adults and their support persons. Methods: This was a mixed-methods study with older adults and their support persons (n = 10 dyads). Virtual home assistant (VHA) equipment was installed in participants’ homes, and its use was documented for 60 days. Participants received protocol-guided telephone calls to address their VHA questions or problems. The type and frequency of VHA use were summarized with descriptive statistics. End-of-study interviews about VHA use were conducted with dyad participants. Qualitative content analyses were used to describe the interview findings about the dyad’s perceptions of using the VHA, how it was used, any difficulties experienced, and suggestions for future VHA uses. Results: Participant dyads reported positive VHA perceptions, including the potential for VHAs to promote aging in place. Participants discussed the challenges learning the technology and replacing old habits with new ones. Participants offered recommendations for future VHA skills and for more education and training about using the VHA. Conclusions: The study findings suggest that VHAs may be useful for older adults as they age in place and offer reassurance for support persons.
Riverside Greenway in Urban Environment: Residents’ Perception and Use of Greenways along the Huangpu River in Shanghai, China
Authors: Zhao Zheng , Gan Huimin , Qian Xin , Leng Jiahui , Wang Yanbin , Wu Peipei
Published: January 2021

Urban greenways improve green coverage rates in urban environments and transform these environments in a people-oriented manner. This study adopted semantic differential (SD) methods and an importance–performance analysis (IPA) model to evaluate resident perceptions and preferences of riverside greenways. A survey of 588 residents was conducted on typical natural greenways, built greenways, and mixed greenways along the Huangpu River in Shanghai. The results showed that resident perceptions of style, space, and distance differed markedly, whereas their perceptions of environmental and psychological characteristics were relatively similar. There were strong correlations between residents’ characteristics and their perceptions, especially for their perceptions of greenway style, sense of order, and distance from the river. By comparison, most residents preferred mixed greenways. Additionally, respondents from areas with natural and mixed greenways believed that they benefited, whereas those from areas with built greenways displayed a potential sense of deprivation. The results of IPA analysis provide further support for the above conclusions. As a whole, the relatively simple methods demonstrated here could be useful to quantitatively analyze the subjective perceptions of urban residents.
3D Scanners in Orthodontics—Current Knowledge and Future Perspectives—A Systematic Review
Authors: Jedliński Maciej , Mazur Marta , Grocholewicz Katarzyna , Janiszewska-Olszowska Joanna
Published: January 2021

Background: Nowadays the use of intraoral scanners has become a routine practice in orthodontics. It allows the introduction of many treatment innovations. One should consider to what extent intraoral scanners have influenced the everyday orthodontic practice and in what direction should the further research in this field be conducted. This study is aimed to systematically review and synthesize available controlled trials investigating the accuracy and efficacy of intraoral scanners for orthodontic purpose to provide clinically useful information and to direct further research in this field. Methods: A literature search of free text and MeSH terms was performed by using MedLine (PubMed), Scopus, Web of Science and Embase. The search engines were used to find studies on application of intraoral scanners in orthodontics (from 1950 to 30 September 2020). The following keywords were used: “intraoral scanners AND efficiency AND accuracy AND orthodontics”. Results: The number of potential identified articles was 71, including 61 from PubMed, two from Scopus, three from Web of Science and five from Embase. After removal of duplicates, 67 full-text articles were analyzed for inclusion criteria, 16 of them were selected and finally included in the qualitative synthesis. Conclusions: There are plenty of data available on accuracy and efficacy of different scanners. Scanners of the same generation from different manufacturers have almost identical accuracy. This is the reason why future similar research will not introduce much to the orthodontics. The challenge for the coming years is to find new applications of digital impressions in the orthodontic practice.
Multimodal Imaging Analysis Reveals Frontal-Associated Networks in Relation to Individual Resilience Strength
Authors: Hsieh Shulan , Yao Zai-Fu , Yang Meng-Heng
Published: January 2021

Psychological resilience is regarded as a critical protective factor for preventing the development of mental illness from experienced adverse events. Personal strength is one key element of resilience that reflects an individual’s reactions to negative life events and is crucial for successful adaptation. Previous studies have linked unimodal imaging measures with resilience. However, applying multimodal imaging measures could provide comprehensive organization information at the system level to examine whether an individual’s resilience strength is reflected in the brain’s structural and functional network. In this study, MRI was used to acquire multimodal imaging properties and subscales of personal strength in terms of resilience from 109 participants (48 females and 61 males). We employed a method of fusion independent component analysis to link the association between multimodal imaging components and personal strength of psychological resilience. The results reveal that a fusion component involving multimodal frontal networks in connecting with the parietal, occipital, and temporal regions is associated with the resilience score for personal strength. A multiple regression model further explains the predictive role of frontal-associated regions that cover a visual-related network regulating cognition and emotion to discern the perceived adverse experience. Overall, this study suggests that frontal-associated regions are related to individual resilience strength.