bluuebunny/crossref_metadata_2025 huggingface dataset:
Knowledge, Attitudes and Practices on the Use of Botanical Medicines in a Rural Caribbean Territory
Authors: Vujicic Tatijana , Cohall Damian
Published: October 2021

The worldwide use of medicinal plant products has been steadily increasing over the past few decades, whereas the traditional knowledge and practices of these botanical medicines appears to be diminishing. Considering the need to conserve and document these traditions, the objective of this study was to understand the knowledge, attitudes, and practices of people who are familiar with botanical medicines, as well as any factors that may influence the perceptions and behaviours associated with the use of medicinal plants. A previously validated survey instrument assessing knowledge, attitudes, and practices on the use medicinal plants was randomly administered to residents of three rural Barbadian communities. The data was analyzed using descriptive statistics and cross tabulations (Chi-Square Test, Fisher’s Exact Test), with a confidence level of 95% and significance level ofp< 0.05. One hundred and fifty-four participants completed the survey with a response rate of 96%. From participant responses we found that over 75% of the study population used botanical medicines. Key findings included a diverse repertoire of traditional knowledge on the use of medicinal plants, which included a total of 29 medicinal applications cited across 69 different plant species and 39 families. The most popular species among respondents (irrespective of use) werePimenta racemosa(Mill.) J.W. Moore (FC = 30, RFC = 0.26),Momordica charantiaL. (FC = 28, RFC = 0.24),Zingiber officinaleRoscoe (FC = 22, RFC = 0.19) andAnnona muricataL. (FC = 21, RFC = 0.18). The findings also show the persistence of medico-cultural concepts such as cleansing and cooling, and identified significant associations between the use of botanical medicines and related practices with demographic variables such as education (p= 0.05; Fisher’s Exact Test) and health insurance, χ2(1, n = 152) = 4.645,p= 0.003. The findings of this study can be used in the identification and archiving of the medicinal plant practices in Barbados and the wider Caribbean, as well as for the larger purposes of biocultural exploration, preservation and further scientific assessment of botanical medicine practices.
Crosstalk Between Polygonatum kingianum, the miRNA, and Gut Microbiota in the Regulation of Lipid Metabolism
Authors: Dong Jincai , Gu Wen , Yang Xingxin , Zeng Linxi , Wang Xi , Mu Jiankang , Wang Yanfang , Li Fengjiao , Yang Min , Yu Jie
Published: October 2021

Objectives:Polygonatum kingianum is a medicinal herb used in various traditional Chinese medicine formulations. The polysaccharide fraction of P. kingianum can reduce insulin resistance and restore the gut microbiota in a rat model of aberrant lipid metabolism by down regulating miR-122. The aim of this study was to further elucidate the effect of P. kingianum on lipid metabolism, and the roles of specific miRNAs and the gut microbiota.

Key findings:P. kingianum administration significantly altered the abundance of 29 gut microbes and 27 differentially expressed miRNAs (DEMs). Several aberrantly expressed miRNAs closely related to lipid metabolism were identified, of which some were associated with specific gut microbiota. MiR-484 in particular was identified as the core factor involved in the therapeutic effects of P. kingianum. We hypothesize that the miR-484-Bacteroides/Roseburia axis acts as an important bridge hub that connects the entire miRNA-gut microbiota network. In addition, we observed that Parabacteroides and Bacillus correlated significantly with several miRNAs, including miR-484, miR-122-5p, miR-184 and miR-378b.

Summary:P. kingianum alleviates lipid metabolism disorder by targeting the network of key miRNAs and the gut microbiota.
CBD Effects on Motor Profile and Neurobiological Indices Related to Glutamatergic Function Induced by Repeated Ketamine Pre-Administration
Authors: Poulia Nafsika , Delis Foteini , Brakatselos Charalampos , Ntoulas George , Asprogerakas Michail-Zois , Antoniou Katerina
Published: October 2021

Clinical evidence and experimental studies have shown the psychotomimetic properties induced by ketamine. Moreover, acute or chronic ketamine (KET) administration has been widely used for modeling schizophrenia-like symptomatology and pathophysiology. Several studies have reported the antipsychotic potential of cannabidiol (CBD), while there is limited information on the cannabidiol effect on KET-induced schizophrenia-like impairments. Therefore, the goal of the present study was to evaluate neuroplastic changes induced by repeated KET administration, which is used as an experimental model of schizophrenia—with a behavioral focus on positive-like symptomatology– and to assess the modulatory role of CBD treatment. The present findings have shown a robust increase in motor activity in KET-treated rats, following a 10-day period of chronic administration at the sub-anesthetic dose of 30 mg/kg (i.p), that was reversed to normal by subsequent chronic CBD treatment. Concerning the expression of glutamate receptors, the current findings have shown region-dependent KET-induced constitutional alterations in NMDA and AMPA receptors that were modified by subsequent CBD treatment. Additionally, repeated KET administration increased ERK1/2 phosphorylation state in all regions examined, apart from the ventral hippocampus that was modulated by subsequent CBD treatment. The present results show, for the first time, a stimulated motor output coupled with a specific glutamatergic-related status and ERK1/2 activation following chronic KET administration that were attenuated by CBD treatment, in a region-dependent manner. These findings provide novel information concerning the antipsychotic potential of CBD using a specific design of chronic KET administration, thus contributing to experimental approaches that mirror the symptomatology and pathophysiology of schizophrenia.
Factors Associated With the Acceleration of Patient Enrollment in Clinical Studies: A Cross-Sectional Study
Authors: Ueda Rieko , Nishizaki Yuji , Nojiri Shuko , Iwata Hiroshi , Miyauchi Katsumi , Matsuyama Kotone , Sanada Shoji , Minamino Tohru , Daida Hiroyuki
Published: October 2021

Under-recruitment in clinical trials is an issue worldwide. If the number of patients enrolled is lower than expected, based on the required sample size, then the reliability of the study results and their validation tend to be impaired. The current study therefore evaluated factors associated with accelerating patient enrollment using data from an ongoing multicenter prospective cohort study. The researchers encouraged research institutions to accelerate patient enrollment via e-mail, newsletters, telephone calls, and site visits. We analyzed the relationship between several potential factors associated with acceleration of patient enrollment including site visits and patient enrollment in a real clinical study. Data were collected from 106 research institutions that participated in a multicenter prospective cohort study. Results showed that the following parameters differed in terms of patient enrollment and non-enrollment: urban area (47.2 vs. 67.6%, p = 0.04), clinical research coordinator (CRC) participation in data input to electronic data capture (EDC) (41.7 vs. 11.8%, p < 0.01), and site visit (38.9 vs. 11.8%, p < 0.01). A multivariate analysis revealed that patient enrollment was significantly associated with urban area (odds ratio [OR] 0.33, 95% confidence interval [CI] 0.12–0.86, p = 0.02), CRC participation in data input to EDC (OR 5.02; 95% CI 1.49–16.8; p < 0.01), and site visit (OR 4.54, 95% CI 1.31–15.7, p = 0.01). In conclusion, site visits and CRC participation in data input to EDC had a significant effect on patient enrollment promotion. Moreover, hospitals in rural areas were more effective in promoting patient enrollment than those in urban areas.
Chinese Patent Medicine Liuweiwuling Tablet had Potent Inhibitory Effects on Both Wild-Type and Entecavir-Resistant Hepatitis B Virus (HBV) in vitro and Effectively Suppressed HBV Replication in Mouse Model
Authors: Ge Fei-lin , Si Lan-lan , Yang Yan , Li Yuan-hua , Lv Zhong-lin , Liu Wen-hui , Liao Hao , Wang Jun , Zou Jun , Li Le , Li Hui , Zhang Zi-lin , Wang Jia-bo , Lu Xue-chun , Xu Dong-ping , Bai Zhao-fang , Liu Yan , Xiao Xiao-he
Published: October 2021

Liuweiwuling Tablet (LWWL) is a licensed Chinese patent medicine (approval number: Z20060238) included in the national health insurance for anti-inflammation of chronic HBV infection, whereas its anti-HBV effect remains clarification. The study aimed to clarify its antiviral effect and related mechanisms. HepG2.2.15 cells (wild-type HBV-replicating cells) and HepG2. A64 cells (entecavir-resistant HBV-replicating cells) were used for in vitro test. Hydrodynamic injection-mediated HBV-replicating mouse model was used for in vivo test. Active compounds and related mechanisms for antiviral effect of LWWL were analyzed using network pharmacology and transcriptomics. The inhibition rates of LWWL (0.8 mg/ml) on HBV DNA, HBsAg, and pgRNA were 57.06, 38.55, and 62.49% in HepG2.2.15 cells, and 51.57, 17.57, and 53.88% in HepG2. A64 cells, respectively. LWWL (2 g kg−1 d−1 for 4 weeks)-treated mice had 1.16 log10 IU/mL decrease of serum HBV DNA, and more than 50% decrease of serum HBsAg/HBeAg and hepatic HBsAg/HBcAg. Compared to tenofovir control, LWWL was less effective in suppressing HBV DNA but more effective in suppressing HBV antigens. Thirteen differentially-expressed genes were found in relation to HBV-host interaction and some of them were enriched in interferon (IFN)-β pathway in LWWL-treated HepG2.2.15 cells. CD3+CD4+ T-cell frequency and serum IFN-γ were significantly increased in LWWL-treated mice compared to LWWL-untreated mice. Among 26 compounds with potential anti-HBV effects that were predicted by network pharmacology, four compounds (quercetin, luteolin, wogonin, and kaempferol) were experimentally confirmed to have antiviral potency. In conclusion, LWWL had potent inhibitory effect on both wild-type and entecavir-resistant HBV, which might be associated with increasing IFN-β and IFN-γ production.
Antidiabetic Medicinal Plants Used in Democratic Republic of Congo: A Critical Review of Ethnopharmacology and Bioactivity Data
Authors: Kasali Félicien Mushagalusa , Kadima Justin Ntokamunda , Peter Emanuel L. , Mtewa Andrew G. , Ajayi Clement Olusoji , Tusiimire Jonans , Tolo Casim Umba , Ogwang Patrick Engeu , Weisheit Anke , Agaba Amon Ganafa
Published: October 2021

Several studies have been conducted and published on medicinal plants used to manage Diabetes Mellitus worldwide. It is of great interest to review available studies from a country or a region to resort to similarities/discrepancies and data quality. Here, we examined data related to ethnopharmacology and bioactivity of antidiabetic plants used in the Democratic Republic of Congo. Data were extracted from Google Scholar, Medline/PubMed, Scopus, ScienceDirect, the Wiley Online Library, Web of Science, and other documents focusing on ethnopharmacology, pharmacology, and phytochemistry antidiabetic plants used in the Democratic Republic of Congo from 2005 to September 2021. The Kew Botanic Royal Garden and Plants of the World Online web databases were consulted to verify the taxonomic information. CAMARADES checklist was used to assess the quality of animal studies and Jadad scores for clinical trials. In total, 213 plant species belonging to 72 botanical families were reported. Only one plant, Droogmansia munamensis, is typically native to the DRC flora; 117 species are growing in the DRC and neighboring countries; 31 species are either introduced from other regions, and 64 are not specified. Alongside the treatment of Diabetes, about 78.13% of plants have multiple therapeutic uses, depending on the study sites. Experimental studies explored the antidiabetic activity of 133 plants, mainly in mice, rats, guinea pigs, and rabbits. Several chemical classes of antidiabetic compounds isolated from 67 plant species have been documented. Rare phase II clinical trials have been conducted. Critical issues included poor quality methodological protocols, author name incorrectly written (16.16%) or absent (14.25%) or confused with a synonym (4.69%), family name revised (17.26%) or missing (1.10%), voucher number not available 336(92.05%), ecological information not reported (49.59%). Most plant species have been identified and authenticated (89.32%). Hundreds of plants are used to treat Diabetes by traditional healers in DRC. However, most plants are not exclusively native to the local flora and have multiple therapeutic uses. The analysis showed the scarcity or absence of high-quality, in-depth pharmacological studies. There is a need to conduct further studies of locally specific species to fill the gap before their introduction into the national pharmacopeia.
Heterogeneous Preferential Attachment in Key Ethereum-Based Cryptoassets
Authors: De Collibus Francesco Maria , Partida Alberto , Piškorec Matija , Tessone Claudio J.
Published: October 2021

In this study, we analyse the aggregated transaction networks of Ether (the native cryptocurrency in Ethereum) and the three most market-capitalised ERC-20 tokens in this platform at the time of writing: Binance, USDT, and Chainlink. We analyse a comprehensive dataset from 2015 to 2020 (encompassing 87,780,546 nodes and 856,207,725 transactions) to understand the mechanism that drives their growth. In a seminal analysis, Kondor et al. (PLoS ONE, 2014, 9: e86197) showed that during its first year, the aggregated Bitcoin transaction network grew following linear preferential attachment. For the Ethereum-based cryptoassets, we find that they present in general super-linear preferential attachment, i.e., the probability for a node to receive a new incoming link is proportional to kα, where k is the node’s degree. Specifically, we find an exponent α = 1.2 for Binance and Chainlink, for Ether α = 1.1, and for USDT α = 1.05. These results reveal that few nodes become hubs rapidly. We then analyse wealth and degree correlation between tokens since many nodes are active simultaneously in different networks. We conclude that, similarly to what happens in Bitcoin, “the rich indeed get richer” in Ethereum and related tokens as well, with wealth much more concentrated than in-degree and out-degree.
Tenascin-C: Friend or Foe in Lung Aging?
Authors: Gremlich Sandrine , Cremona Tiziana P. , Yao Eveline , Chabenet Farah , Fytianos Kleanthis , Roth-Kleiner Matthias , Schittny Johannes C.
Published: October 2021

Lung aging is characterized by lung function impairment, ECM remodeling and airspace enlargement. Tenascin-C (TNC) is a large extracellular matrix (ECM) protein with paracrine and autocrine regulatory functions on cell migration, proliferation and differentiation. This matricellular protein is highly expressed during organogenesis and morphogenetic events like injury repair, inflammation or cancer. We previously showed that TNC deficiency affected lung development and pulmonary function, but little is known about its role during pulmonary aging. In order to answer this question, we characterized lung structure and physiology in 18 months old TNC-deficient and wild-type (WT) mice. Mice were mechanically ventilated with a basal and high tidal volume (HTV) ventilation protocol for functional analyses. Additional animals were used for histological, stereological and molecular biological analyses. We observed that old TNC-deficient mice exhibited larger lung volume, parenchymal volume, total airspace volume and septal surface area than WT, but similar mean linear intercept. This was accompanied by an increase in proliferation, but not apoptosis or autophagy markers expression throughout the lung parenchyma. Senescent cells were observed in epithelial cells of the conducting airways and in alveolar macrophages, but equally in both genotypes. Total collagen content was doubled in TNC KO lungs. However, basal and HTV ventilation revealed similar respiratory physiological parameters in both genotypes. Smooth muscle actin (α-SMA) analysis showed a faint increase in α-SMA positive cells in TNC-deficient lungs, but a marked increase in non-proliferative α-SMA + desmin + cells. Major TNC-related molecular pathways were not up- or down-regulated in TNC-deficient lungs as compared to WT; only minor changes in TLR4 and TGFβR3 mRNA expression were observed. In conclusion, TNC-deficient lungs at 18 months of age showed exaggerated features of the normal structural lung aging described to occur in mice between 12 and 18 months of age. Correlated to the increased pulmonary function parameters previously observed in young adult TNC-deficient lungs and described to occur in normal lung aging between 3 and 6 months of age, TNC might be an advantage in lung aging.
Reference Gene Selection for Normalizing Gene Expression in Ips Sexdentatus (Coleoptera: Curculionidae: Scolytinae) Under Different Experimental Conditions
Authors: Sellamuthu Gothandapani , Amin Shan , Bílý Jan , Synek Jirí , Modlinger Roman , Sen Madhab Kumar , Chakraborty Amrita , Roy Amit
Published: October 2021

Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae) is one of the most destructive and economically important forest pests. A better understanding of molecular mechanisms underlying its adaptation to toxic host compounds may unleash the potential for future management of this pest. Gene expression studies could be considered as one of the key experimental approaches for such purposes. A suitable reference gene selection is fundamental for quantitative gene expression analysis and functional genomics studies in I. sexdentatus. Twelve commonly used reference genes in Coleopterans were screened under different experimental conditions to obtain accurate and reliable normalization of gene expression data. The majority of the 12 reference genes showed a relatively stable expression pattern among developmental stages, tissue-specific, and sex-specific stages; however, some variabilities were observed during varied temperature incubation. Under developmental conditions, the Tubulin beta-1 chain (β-Tubulin) was the most stable reference gene, followed by translation elongation factor (eEF2) and ribosomal protein S3 (RPS3). In sex-specific conditions, RPS3, β-Tubulin, and eEF2 were the most stable reference genes. In contrast, different sets of genes were shown higher stability in terms of expression under tissue-specific conditions, i.e., RPS3 and eEF2 in head tissue, V-ATPase-A and eEF2 in the fat body, V-ATPase-A and eEF2 in the gut. Under varied temperatures, β-Tubulin and V-ATPase-A were most stable, whereas ubiquitin (UbiQ) and V-ATPase-A displayed the highest expression stability after Juvenile Hormone III treatment. The findings were validated further using real-time quantitative reverse transcription PCR (RT-qPCR)-based target gene expression analysis. Nevertheless, the present study delivers a catalog of reference genes under varied experimental conditions for the coleopteran forest pest I. sexdentatus and paves the way for future gene expression and functional genomic studies on this species.
Comparative Transcriptome Analysis Reveals the Mechanisms Underlying Differences in Salt Tolerance Between indica and japonica Rice at Seedling Stage
Authors: Kong Weilong , Sun Tong , Zhang Chenhao , Deng Xiaoxiao , Li Yangsheng
Published: October 2021

Screening and breeding more salt-tolerant varieties is an effective way to deal with the global reduction in rice (Oryza sativa L.) yield caused by salt stress. However, the molecular mechanism underlying differences in salt tolerance between varieties, especially between the subspecies, is still unclear. We herein performed a comparative transcriptomic analysis under salt stress in contrasting two rice genotypes, namely RPY geng (japonica, tolerant variety) and Luohui 9 (named as Chao 2R in this study, indica, susceptible variety). 7208 and 3874 differentially expressed genes (DEGs) were identified under salt stress in Chao 2R and RPY geng, separately. Of them, 2714 DEGs were co-expressed in both genotypes, while 4494 and 1190 DEGs were specifically up/down-regulated in Chao 2R and RPY geng, respectively. Gene ontology (GO) analysis results provided a more reasonable explanation for the salt tolerance difference between the two genotypes. The expression of normal life process genes in Chao 2R were severely affected under salt stress, but RPY geng regulated the expression of multiple stress-related genes to adapt to the same intensity of salt stress, such as secondary metabolic process (GO:0019748), oxidation-reduction process (GO:0009067), etc. Furthermore, we highlighted important pathways and transcription factors (TFs) related to salt tolerance in RPY geng specific DEGs sets based on MapMan annotation and TF identification. Through Meta-QTLs mapping and homologous analysis, we screened out 18 salt stress-related candidate genes (RPY geng specific DEGs) in 15 Meta-QTLs. Our findings not only offer new insights into the difference in salt stress tolerance between the rice subspecies but also provide critical target genes to facilitate gene editing to enhance salt stress tolerance in rice.
Super Rice With High Sink Activities Has Superior Adaptability to Low Filling Stage Temperature
Authors: Xu Congshan , Yang Fei , Tang Xinao , Lu Bo , Li Ziyu , Liu Zhenghui , Ding Yanfeng , Ding Chao , Li Ganghua
Published: October 2021

To investigate the differential responses of super rice grain filling to low filling stage temperature (LT) and the regulative effect of nitrogen panicle fertilizer (NPF), physiological and molecular experiments were conducted with two super rice varieties (Nanjing 7th: N7 and Nanjing 9108th: N9108) on two different filling stage temperature treatments implemented by applying two sowing dates [Normal filling stage temperature (CK): Sowed on May 30, Tmean = 24.7°C and low filling stage temperature (LT): Sowed on July 1, Tmean = 20.3°C], and two NPF levels (0 and 150 kg N ha−1). In this study, LT, NPF, and simultaneous LT and NPF treatments suppressed the grain filling in all varieties with different levels. Under LT or NPF treatments, the reduction of grain weight, seed setting rate, and filling rate were closely associated with suppressed starch biosynthesis rate in inferior seeds, suggesting that reduced starch biosynthesis rate, expression, and activities of enzymes encoded by related genes, Floury endosperm-4 (FLO4), Starch branching enzyme-I (SBE1), and Starch phosphorylase-L (PHO-l), were responsible for the grain filling reduction. Under LT or NPF treatments, significantly higher grain filling rates and lower variance were found in N9108 compared to that in N7, which were closely related to their higher starch biosynthesis ability, related gene expression, and enzymes activities. One of the probable explanations of the grain filling difference was the variation in the relative amount of key regulative hormones, Abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC). These results raise a possibility that super rice with higher sink activities has superior adaptability to LT and NPF due to their higher sink activities.
The Wheat Wall-Associated Receptor-Like Kinase TaWAK-6D Mediates Broad Resistance to Two Fungal Pathogens Fusarium pseudograminearum and Rhizoctonia cerealis
Authors: Qi Haijun , Guo Feilong , Lv Liangjie , Zhu Xiuliang , Zhang Li , Yu Jinfeng , Wei Xuening , Zhang Zengyan
Published: October 2021

The soil-borne fungi Fusarium pseudograminearum and Rhizoctonia cerealis are the major pathogens for the economically important diseases Fusarium crown rot (FCR) and sharp eyespot of common wheat (Triticum aestivum), respectively. However, there has been no report on the broad resistance of wheat genes against both F. pseudograminearum and R. cerealis. In the current study, we identified TaWAK-6D, a wall-associated kinase (WAK) which is an encoding gene located on chromosome 6D, and demonstrated its broad resistance role in the wheat responses to both F. pseudograminearum and R. cerealis infection. TaWAK-6D transcript induction by F. pseudograminearum and R. cerealis was related to the resistance degree of wheat and the gene expression was significantly induced by exogenous pectin treatment. Silencing of TaWAK-6D compromised wheat resistance to F. pseudograminearum and R. cerealis, and repressed the expression of a serial of wheat defense-related genes. Ectopic expression of TaWAK-6D in Nicotiana benthamiana positively modulated the expression of several defense-related genes. TaWAK-6D protein was determined to localize to the plasma membrane in wheat and N. benthamiana. Collectively, the TaWAK-6D at the plasma membrane mediated the broad resistance responses to both F. pseudograminearum and R. cerealis in wheat at the seedling stage. This study, therefore, concludes that TaWAK-6D is a promising gene for improving wheat broad resistance to FCR and sharp eyespot.
Quantitative Trait Locus Analysis and Identification of Candidate Genes for Micronaire in an Interspecific Backcross Inbred Line Population of Gossypium hirsutum × Gossypium barbadense
Authors: Pei Wenfeng , Song Jikun , Wang Wenkui , Ma Jianjiang , Jia Bing , Wu Luyao , Wu Man , Chen Quanjia , Qin Qin , Zhu Haiyong , Hu Chengcheng , Lei Hai , Gao Xuefei , Hu Haijun , Zhang Yu , Zhang Jinfa , Yu Jiwen , Qu Yanying
Published: October 2021

Cotton is the most important fiber crop and provides indispensable natural fibers for the textile industry. Micronaire (MIC) is determined by fiber fineness and maturity and is an important component of fiber quality. Gossypium barbadense L. possesses long, strong and fine fibers, while upland cotton (Gossypium hirsutum L.) is high yielding with high MIC and widely cultivated worldwide. To identify quantitative trait loci (QTLs) and candidate genes for MIC in G. barbadense, a population of 250 backcross inbred lines (BILs), developed from an interspecific cross of upland cotton CRI36 × Egyptian cotton (G. barbadense) Hai7124, was evaluated in 9 replicated field tests. Based on a high-density genetic map with 7709 genotyping-by-sequencing (GBS)-based single-nucleotide polymorphism (SNP) markers, 25 MIC QTLs were identified, including 12 previously described QTLs and 13 new QTLs. Importantly, two stable MIC QTLs (qMIC-D03-2 on D03 and qMIC-D08-1 on D08) were identified. Of a total of 338 genes identified within the two QTL regions, eight candidate genes with differential expression between TM-1 and Hai7124 were identified. Our research provides valuable information for improving MIC in cotton breeding.
Space Breeding: The Next-Generation Crops
Authors: Mohanta Tapan Kumar , Mishra Awdhesh Kumar , Mohanta Yugal Kishore , Al-Harrasi Ahmed
Published: October 2021

Since the beginning of space exploration, researchers have been exploring the role of microgravity, cosmic radiation, and other aspects of the space environment on plant growth and development. To create superior crop varieties and achieve noticeable success in the space environment, several types of research have been conducted thus far. Space-grown plants have been exposed to cosmic radiation and microgravity, which has led to the generation of crop varieties with diverse genotypes and phenotypes arising from different cellular, subcellular, genomic, chromosomal, and biochemical changes. DNA damage and chromosomal aberrations due to cosmic radiation are the major factors responsible for genetic polymorphism and the generation of crops with modified genetic combinations. These changes can be used to produce next-generation crop varieties capable of surviving diverse environmental conditions. This review aims to elucidate the detailed molecular mechanisms and genetic mutations found in plants used in recent space crop projects and how these can be applied in space breeding programmes in the future.
Decision-Making and the Alternative Five Factor Personality Model: Exploring the Role of Personality Traits, Age, Sex and Social Position
Authors: Urieta Patricia , Aluja Anton , Garcia Luis F. , Balada Ferran , Lacomba Elena
Published: October 2021

This study explores the relationship between decision-making style, as measured by the Melbourne Decision Making Questionnaire, and personality based on alternative five-factor model along with effect of age, sex and social position on such styles. A large sample of community and undergraduate students (n= 1,562; Mage= 40.03,SD= 18.43) was analyzed. The results showed that Neuroticism and Extraversion were significantly related to the non-vigilant styles Hypervigilance, Buck-passing and Procrastination. Women scored significantly lower in Vigilance and higher in Hypervigilance, Buck-passing and Procrastinations than men. Age was significantly related to decision-making style in a U-shaped fashion. The Social Position Index was significantly related to all decision-making styles. The most predictive personality domains regarding decision-making scales were Aggressiveness (negatively) and Activity for Vigilance, and Neuroticism for Hypervigilance, Buck-passing and Procrastination. Age, sex and social position had a small/medium overall effect on the four dimensions of Melbourne Decision Making Questionnaire (p< 0.001) with a η2of 0.038, 0.068, 0.050, and 0.031 for Vigilance, Hypervigilance, Buck-passing and Procrastination, respectively. Based on scores on a single factor dimension of the MDMQ, the profile of participants with higher scores was characterized by lower age, more likely to be females, lower social position, higher levels of Aggressiveness, less Activity, less Extraversion, and higher Neuroticism.
Validating the “Two Faces” of Envy: The Effect of Self-Control
Authors: Yang Chen , Tang Rixin
Published: October 2021

Envy drives different behaviors, and while we often emphasize the negative effects of envy, there are also relatively positive aspects. This study explored the “two faces” of envy or behaviors that improve oneself or degrade others. In study 1 (N=466, 45.1% males and 54.9% females; Mage=18.53, SDage=2.05), we modeled the effects of envy and self-control on effort and aggression. In study 2 (N=102, 51% males and 49% females; Mage=20.56, SDage=1.88), we explored the influence of envy on striving behavior and aggressive behavior using an ego depletion paradigm. The different effects of envy on different levels were doubly verified. We established structural equation models of the interactions of benign envy, malicious envy, self-control, and associated behaviors, and we found that: (1) Individuals’ striving behavior was only affected by benign envy; (2) Individuals’ aggressive behavior was influenced by both malicious envy and self-control. Ego depletion moderated the effect of malicious envy on aggressive behavior.
Examining Digital Entrepreneurship: The Goal of Optimization of Transformation Path Normal Education in China
Authors: Zhao Yang
Published: October 2021

The education system in China needs optimization with the erupted pandemic for effective outcomes. The path for normal education is upgrading itself with online learning, hence offering a challenge for entrepreneurship. The education sector needs to tackle these offered challenges better that optimizes and exploits the situations. The way teachers and students communicate and utilize their learning to materialize new ideas is very important for keeping pace. Therefore, this study is aimed to investigate the role of mentorship in digital entrepreneurship. The population for the study was the teachers of normal education in China. The sampling design used was convenient random sampling, and data were collected with a self-administered questionnaire on five points Likert scale. This study has used Smart PLS 3.3.3 (USA) for the data analysis through structural equation modeling. In the first stage, the instrument analyzed the measurement model, and in the second stage, the hypotheses were checked using the data collected. The findings of the study show that mentorship plays a very important role in knowledge sharing and innovation, which further leads to digital entrepreneurship. The study will open a new path in the education field to incorporate knowledge hidings and transformational entrepreneurship.
Effects of the Application of Virtual Reality to Experiential Education on Self-Efficacy and Learning Motivation of Social Workers
Authors: Hsiao Suh Chen
Published: October 2021

To enhance the human resources required for national development to cope with the change, countries in the world have positively invested in education, as national education in the future is necessary to cultivate new-generation citizens with new traits and abilities to cope with the possible impacts and challenges in the new century. For this reason, the education reform wave sweeps many countries. The experiential learning model in experiential education nowadays leads profit and non-profit organizations in the business community, education, and social worker groups to the alternative education trend. Various experiential learning curricula are therefore spread. Taking social workers in southern Taiwan as the research objects, a total of 227 social workers are preceded the 15-week (3 h per week for a total of 45 h) experimental research with the application of virtual reality to experiential education. The research results summarize that (1) experiential education with virtual reality would affect self-efficacy, (2) experiential education with virtual reality would affect learning motivation, and (3) self-efficacy reveals remarkably positive effects on learning motivation. According to the results, it is expected to increase the interaction among the social workers through the learning activity and internalize the experience in the practical learning process of communication, problem solving, and extrinsic interaction for the application to the work to achieve a better life.
Functional Constructivism Approach to Multilevel Nature of Bio-Behavioral Diversity
Authors: Trofimova Irina
Published: October 2021

Attempts to revise the existing classifications of psychiatric disorders (DSM and ICD) continue and highlight a crucial need for the identification of biomarkers underlying symptoms of psychopathology. The present review highlights the benefits of using a Functional Constructivism approach in the analysis of the functionality of the main neurotransmitters. This approach explores the idea that behavior is neither reactive nor pro-active, but constructive and generative, being a transient selection of multiple degrees of freedom in perception and actions. This review briefly describes main consensus points in neuroscience related to the functionality of eight neurochemical ensembles, summarized as a part of the neurochemical model Functional Ensemble of Temperament (FET). None of the FET components is represented by a single neurotransmitter; all neurochemical teams have specific functionality in selection of behavioral degrees of freedom and stages of action construction. The review demonstrates the possibility of unifying taxonomies of temperament and classifications of psychiatric disorders and presenting these taxonomies formally and systematically. The paper also highlights the multi-level nature of regulation of consistent bio-behavioral individual differences, in line with the concepts of diagonal evolution (proposed earlier) and Specialized Extended Phenotype.
Prevalence of Autism Spectrum Disorder and Co-morbidities in Children and Adolescents: A Systematic Literature Review
Authors: Bougeard Clémence , Picarel-Blanchot Françoise , Schmid Ramona , Campbell Rosanne , Buitelaar Jan
Published: October 2021

Objective: Individuals with autism spectrum disorder often present somatic and/or psychiatric co-morbid disorders. The DSM-5 allows for consideration of additional diagnoses besides ASD and may have impacted the prevalence of co-morbidities as well as being limited in capturing the true differences in prevalence observed between males and females. We describe the prevalence of ASD and frequently observed co-morbidities in children and adolescents (<18 years) in the United States and five European countries.

Methods: Two systematic literature reviews were conducted in PubMed and Embase for the period 2014–2019 and focusing on the prevalence of ASD and nine co-morbidities of interest based on their frequency and/or severity: Attention Deficit Hyperactivity Disorder (ADHD), anxiety, depressive disorders, epilepsy, intellectual disability (ID), sleep disorders, sight/hearing impairment/loss, and gastro-intestinal syndromes (GI).

Results: Thirteen studies on prevalence of ASD and 33 on prevalence of co-morbidities were included. Prevalence of ASD was 1.70 and 1.85% in US children aged 4 and 8 years respectively, while prevalence in Europe ranged between 0.38 and 1.55%. Additionally, current evidence is supportive of a global increase in ASD prevalence over the past years. Substantial heterogeneity in prevalence of co-morbidities was observed: ADHD (0.00–86.00%), anxiety (0.00–82.20%), depressive disorders (0.00–74.80%), epilepsy (2.80–77.50%), ID (0.00–91.70%), sleep disorders (2.08–72.50%), sight/hearing impairment/loss (0.00–14.90%/0.00–4.90%), and GI syndromes (0.00–67.80%). Studies were heterogeneous in terms of design and method to estimate prevalence. Gender appears to represent a risk factor for co-morbid ADHD (higher in males) and epilepsy/seizure (higher in females) while age is also associated with ADHD and anxiety (increasing until adolescence).

Conclusion: Our results provide a descriptive review of the prevalence of ASD and its co-morbidities in children and adolescents. These insights can be valuable for clinicians and parents/guardians of autistic children. Prevalence of ASD has increased over time while co-morbidities bring additional heterogeneity to the clinical presentation, which further advocates for personalized approaches to treatment and support. Having a clear understanding of the prevalence of ASD and its co-morbidities is important to raise awareness among stakeholders.
Contactless In-Home Monitoring of the Long-Term Respiratory and Behavioral Phenotypes in Older Adults With COVID-19: A Case Series
Authors: Zhang Guo , Vahia Ipsit V. , Liu Yingcheng , Yang Yuzhe , May Rose , Cray Hailey V. , McGrory William , Katabi Dina
Published: October 2021

Currently, there is a limited understanding of long-term outcomes of COVID-19, and a need for in-home measurements of patients through the whole course of their disease. We study a novel approach for monitoring the long-term trajectories of respiratory and behavioral symptoms of COVID-19 patients at home. We use a sensor that analyzes the radio signals in the room to infer patients' respiration, sleep and activities in a passive and contactless manner. We report the results of continuous monitoring of three residents of an assisted living facility for 3 months, through the course of their disease and subsequent recovery. In total, we collected 4,358 measurements of gait speed, 294 nights of sleep, and 3,056 h of respiration. The data shows differences in the respiration signals between asymptomatic and symptomatic patients. Longitudinally, we note sleep and motor abnormalities that persisted for months after becoming COVID negative. Our study represents a novel phenotyping of the respiratory and behavioral trajectories of COVID recovery, and suggests that the two may be integral components of the COVID-19 syndrome. It further provides a proof-of-concept that contactless passive sensors may uniquely facilitate studying detailed longitudinal outcomes of COVID-19, particularly among older adults.
Age and Ageing During the COVID-19 Pandemic; Challenges to Public Health and to the Health of the Public
Authors: Clarfield A. Mark , Dwolatzky Tzvi
Published: October 2021

The distribution of the SARS-CoV-2 virus has reached pandemic proportions. While COVID-19 can affect anyone, it is particularly hazardous for those with “co-morbidities.” Older age is an especially strong and independent risk factor for hospital and ICU admission, mechanical ventilation and death. Health systems must protect persons at any age while paying particular attention to those with risk factors. However, essential freedoms must be respected and social/psychological needs met for those shielded. The example of the older population in Israel may provide interesting public health lessons. Relatively speaking, Israel is a demographically young country, with only 11.5% of its population 65 years and older as compared with the OECD average of >17%. As well, a lower proportion of older persons is in long-term institutions in Israel than in most other OECD countries. The initiation of a national program to protect older residents of nursing homes and more latterly, a successful vaccine program has resulted in relatively low rates of serious COVID-19 related disease and mortality in Israel. However, the global situation remains unstable and the older population remains at risk. The rollout of efficacious vaccines is in progress but it will probably take years to cover the world's population, especially those living in low- and middle-income countries. Every effort must be made not to leave these poorer countries behind. Marrying the principles of public health (care of the population) with those of geriatric medicine (care of the older individual) offers the best way forward.
Chinese Parent Intention to Vaccinate Children With Special Diseases Against COVID-19
Authors: Wang Xu , Yan Wu , Lu Lingling , Cao Liming , Tian Ye , Zhou Kai
Published: October 2021

Background: Information on the intention of parents of children with special diseases to vaccinate their children against coronavirus disease 2019 (COVID-19) is scarce.

Methods: In this survey, all participants (n = 914) were enrolled from a tertiary children's hospital between September 2020 and April 2021. A face-to-face questionnaire interview was conducted to collect information on the special diseases of children and parental attitudes about the COVID-19 vaccine. We compared the demographic and disease factors between the group of parents who were willing to vaccinate their children against COVID-19 and the group who were unwilling to vaccinate.

Results: Among 941 children, 58.1% (n = 547) were boys. The Mean age was 1.4 (SD 1.9) years. If the COVID-19 vaccine becomes available for the child, 470 (49.9%) of parents were willing to provide vaccination for their children. The less the education levels of the father or mother, the more likely they were to vaccinate their children (P = 0.003, P = 0.007). However, more intentions to vaccinate were provided in parents of children with COVID-19 prevention and control education (P < 0.001).

Conclusion: Our findings provided evidence that some parents are willing to vaccinate their children with special diseases against COVID-19. Professional knowledge about COVID-19 prevention and control may contribute to increased parental intention.
The Impact of Smoking on Subgingival Plaque and the Development of Periodontitis: A Literature Review
Authors: Zhang Jiaxin , Yu Jialu , Dou Jinge , Hu Pingyue , Guo Qiang
Published: October 2021

Smoking seriously affects oral health and causes a variety of oral diseases. Numerous clinical data show that smoking significantly increases the risk of periodontitis, and the duration and amount of smoking are positively correlated with the severity of periodontitis. In fact, smoking creates an environment conducive to the colonization of periodontopathogens, which affects the process of periodontitis. Since subgingival plaque which harbors periodontopathogens is the initiation factor of periodontitis, it is critical to study the impact of smoking on subgingival microbiota for understanding the relationship between smoking and periodontitis. Continuous advances have been made on the understanding of effects of smoking on subgingival plaque and the development of periodontitis. Smoking is observed to enhance the pathogenicity of periodontopathogens, especially the red complex microorganisms, via promoting their colonization and infection, and regulating the expression and function of multiple virulence factors. Furthermore, smoking has a negative impact on periodontal microecological homeostasis, which is reflected in the decrease of commensal bacteria and the increase of periodontopathogens, as well as the changes in the interaction between periodontopathogens and their commensal microbes in subgingival biofilm, thus influencing the pathogenicity of the subgingival plaque. In summary, the mechanism of smoking on subgingival plaque microorganisms represented by the red complex and its effect on the periodontal microecology still need to be further explored. The relevant research results are of great significance for guiding the periodontal clinical treatment of smoking population. This review summarizes the effects and relevant mechanisms of smoking on subgingival plaque and the development of periodontitis.
Mesoscale Features in the Global Geospace Response to the March 12, 2012 Storm
Authors: Adewuyi Mayowa , Keesee Amy M. , Nishimura Yukitoshi , Gabrielse Christine , Katus Roxanne M.
Published: October 2021

The geospace response to coronal mass ejections includes phenomena across many regions, from reconnection at the dayside and magnetotail, through the inner magnetosphere, to the ionosphere, and even to the ground. Phenomena occurring in each region are often connected to each other through the magnetic field, but that field undergoes dynamic changes during storms and substorms. Improving our understanding of the geospace response to storms requires a global picture that enables us to observe all the regions simultaneously with both spatial and temporal resolution. Using the Energetic Neutral Atom (ENA) imager on the Two Wide-Angle Imaging Neutral-Atom Spectrometers (TWINS) mission, a temperature map can be calculated to provide a global view of the magnetotail. These maps are combined with in situ measurements at geosynchronous orbit from GOES 13 and 15, auroral images from all sky imagers (ASIs), and ground magnetometer measurements to examine the global geospace response of a coronal mass ejection (CME) driven event on March 12th, 2012. Mesoscale features in the magnetotail are observed throughout the interval, including prior to the storm commencement and during the main phase, which has implications for the dominant processes that lead to pressure buildup in the inner magnetosphere. Auroral enhancements that can be associated with these magnetotail features through magnetosphere-ionosphere coupling are observed to appear only after global reconfigurations of the magnetic field.