bluuebunny/crossref_metadata_2025 huggingface dataset:
Efektivitas Program Onboarding Sales Assistant untuk Meningkatkan Pengetahuan Produk di PT Kurnia Ciptamoda Gemilang
Authors: Rosadi Nadia Nabila , Syarif Edy
Published: August 2024

Penelitian ini bertujuan untuk mengevaluasi efektivitas program onboarding Sales Assistant PT Kurnia Ciptamoda Gemilang (PT KCG) menggunakan model evaluasi Kirkpatrick. Data dikumpulkan melalui nilai pre-test dan post-test peserta, serta feedback form terkait aktivitas training, modul, dan trainer. Hasil penelitian menunjukkan bahwa program onboarding efektif dalam meningkatkan pengetahuan produk Sales Assistant, dengan rata-rata nilai post-test meningkat secara signifikan dibandingkan nilai pre-test. Feedback dari peserta juga menunjukkan kepuasan terhadap program onboarding, dengan trainer yang dinilai kompeten dan materi training yang relevan. Temuan ini menunjukkan bahwa program onboarding merupakan alat yang efektif untuk meningkatkan pengetahuan produk Sales Assistant di PT KCG.
A ruthenium single atom nanozyme-based antibiotic for the treatment of otitis media caused by Staphylococcus aureus
Authors: Wang Jie , Gong Rui , Yang Ming , Wu Xi , Li Ziwei , Huang Haibing , Yan Xiyun , Wang Daji
Published: August 2024

Staphylococcus aureus (S. aureus) infection is a primary cause of otitis media (OM), the most common disease for which children are prescribed antibiotics. However, the abuse of antibiotics has led to a global increase in antimicrobial resistance (AMR). Nanozymes, as promising alternatives to traditional antibiotics, are being extensively utilized to combat AMR. Here, we synthesize a series of single-atom nanozymes (metal-C3N4 SANzymes) by loading four metals (Ag, Fe, Cu, Ru) with antibacterial properties onto a crystalline g-C3N4. These metal-C3N4 display a rob-like morphology and well-dispersed metal atoms. Among them, Ru-C3N4 demonstrates the optimal peroxidase-like activity (285.3 U mg–1), comparable to that of horseradish peroxidase (267.7 U mg–1). In vitro antibacterial assays reveal that Ru-C3N4 significantly inhibits S. aureus growth compared with other metal-C3N4 even at a low concentration (0.06 mg mL–1). Notably, Ru-C3N4 acts as a narrow-spectrum nanoantibiotic with relative specificity against Gram-positive bacteria. Biofilms formed by S. aureus are easily degraded by Ru-C3N4 due to its high peroxidase-like activity. In vivo, Ru-C3N4 effectively eliminates S. aureus and relieves ear inflammation in OM mouse models. However, untreated OM mice eventually develop hearing impairment. Due to its low metal load, Ru-C3N4 does not exhibit significant toxicity to blood, liver, or kidney. In conclusion, this study presents a novel SANzyme-based antibiotic that can effectively eliminate S. aureus and treat S. aureus-induced OM.
Fracture characterization of fractured rock bodies based on acoustic and optical characteristics
Authors: Zhang Jiyong , Zou Qianjin , Guan Huadong
Published: August 2024

Crack propagation is an important cause of damage to rock bodies. In this study, uniaxial compression tests were conducted on specimens with rock-like mass containing fissures with different inclination angles to study the effect of crack angle on the crack evolution and fracture characteristics of rock bodies. The specimen surface deformation and internal response characteristics during fracture were analyzed via digital image correlation (DIC) and acoustic emission (AE) techniques. The results indicated that the AE characteristics of the fractured specimens exhibited a high degree of activity during the pore compaction and crack propagation stages. The prefabricated fissure configuration affected the stress state at the fissure tip, leading to differences in the crack evolution paths and rupture modes of fissure specimens with different angles. Under the uniaxial peak intensity, the relative position of the normalized global strain curve peak point gradually shifted from the specimen tip to the middle of the specimen as the crack angle increased, which corresponded to the shear damage-tension-shear mixed damage-tension damage modes of the specimen. The findings of this study indicate that normalized global strain curves can reflect the characteristics of crack evolution and provide a basis for the discrimination of fissured rock mass damage modes.
Economic optimal dispatch of active distribution network with CCHP multi-microgrid based on analytical target cascading
Authors: Yang Yongbiao , Ai Dengxin , Zhang Li , Zheng Yawen
Published: August 2024

When multiple CCHP microgrids are integrated into an active distribution network (ADN), the microgrids and the distribution network serve as distinct stakeholders, making the economic optimal dispatch of the system more complex. This paper proposes a distributed dispatch model of ADN with CCHP multi-microgrid, and refines the objective functions of each region. The analytical target cascading approach (ATC) is employed to model the power transaction as virtual sources/loads, and solve the optimal dispatch in parallel. Case studies demonstrate the proposed distributed model is capable of achieving economic optimization for both stakeholders.
A double-layer optimization strategy for distribution networks considering 5G base station clusters
Authors: Lv Zhipeng , Jia Bingjian , Song Zhenhao , Yang Fei , Zhou Shan
Published: August 2024

The reliability of the power supply for 5G base stations (BSs) is increasing. A large amount of BS backup energy storage (BES) remains underutilized. This study establishes a double-layer optimization distribution network (DN) considering BS clusters. An energy consumption characteristics and scheduling ability model of the BSs was established to address the differences in the characteristics of different traffic flows. A double-tier planning model for BS-joining grid market ancillary services is proposed. The upper-layer model addresses optimal tidal flow problems in DNs to minimize integrated operating costs, while the lower-layer model focuses on BES economic optimization. The double-layer model changes into a single-layer linear model using the Karush–Kuhn–Tucker (KKT) condition and the Big M method. Simulation validation using the IEEE-33 node DN proves that this approach can reduce DN operating costs, regulate voltage fluctuations, and guarantee economical and safe DN operation.
Comparative proteomics analysis of the mouse mini-gut organoid: insights into markers of gluten challenge from celiac disease intestinal biopsies
Authors: Moulder Robert , Bhosale Santosh D. , Viiri Keijo , Lahesmaa Riitta
Published: August 2024

Introduction

Organoid models enable three-dimensional representation of cellular systems, providing flexible and accessible research tools, and can highlight key biomolecules. Such models of the intestinal epithelium can provide significant knowledge for the study of celiac disease and provide an additional context for the nature of markers observed from patient biopsy data.

Methods

Using LC–MS/MS, the proteomes of the crypt and enterocyte-like states of a mouse mini-gut organoid model were measured. The data were further compared with published biopsy data by comparing the changes induced by gluten challenge after a gluten-free diet.

Results and discussion

These analyses identified 4,850 protein groups and revealed how 400 putative biomarkers of dietary challenge were differentially expressed in the organoid model. In addition to the extensive changes within the differentiated cells, the data reiterated the disruption of the crypt–villus axis after gluten challenge. The mass spectrometry data are available via ProteomeXchange with the identifier PXD025690.
Disgust as a primary emotional system and its clinical relevance
Authors: Tolchinsky Alexey , Ellis George F. R. , Levin Michael , Kaňková Šárka , Burgdorf Jeffrey S.
Published: August 2024

This paper advocates for considering disgust as a primary emotional system within Panksepp’s Affective Neuroscience framework, which has the potential to improve the efficacy of psychotherapy with obsessive-compulsive disorder, hypochondriasis, and emetophobia. In 2007, Toronchuk and Ellis provided comprehensive evidence that DISGUST system, as they defined it, matched all Panksepp’s criteria for a primary emotional system. A debate ensued and was not unambiguously resolved. This paper is an attempt to resume this discussion and supplement it with the data that accumulated since then on DISGUST’s relationship with the immune system and the role of DISGUST dysregulation in psychopathology. We hope that renewed research interest in DISGUST has the potential to improve clinical efficacy with hard-to-treat conditions.
Autism and psychopathology - prevalence, identification, and symptoms equivalence: study protocol
Authors: Bertelli Marco O. , Bianco Annamaria , Deb Shoumitro , Scuticchio Daniela , Kaleci Shaniko , Scattoni Maria Luisa
Published: August 2024

Objective

Despite increasing evidence of high psychopathological vulnerability in people with Autism Spectrum Disorder (ASD) and/or Intellectual disability (ID), comprehensive data on prevalence and presentation of psychiatric disorders (PD) in people with significant cognitive and communication impairment are lacking. The extent to which PD can present with behavioral/observable symptoms and include Problem Behaviors (PB) has also been scarcely evaluated through population-based studies. The paper presents the protocol of a cross-sectional study aimed at filling these gaps, referred to a large multicentric Italian population-based sample of adolescents and adults.

Methods

A battery of validated scales, SPAIDD, DASH-II, DiBAS-R, and STA-DI, is used to support and control for clinical diagnoses of PD. Study population is stratified according to different independent variables such as the severity of ID and ASD, gender, age group, and source of recruitment. A network analysis will be carried out to identify the most central behavioral symptoms for the various PD and their relationship with PB. Overlap between psychiatric symptoms and ASD and ID phenotypes is also addressed.

Results and Conclusion

This study should provide valuable insight into better diagnostic accuracy, leading to well-informed interventions to improve the quality of life of people with ASD and/or ID.
Review Quantum Circuit Synthesis for Grover’s Algorithm Oracle
Authors: Naranjo Miguel A. , Fletscher Luis A.
Published: August 2024

The search for information in a system has been a continuous problem for a computer. This has resulted in the construction of a set of classical algorithms that can search for a set of data. This is why search systems can be divided into the type of information being searched, the number of solutions to find, and even the terms used for searching. With the emergence of quantum computing, new algorithms have been generated for this type of process. An example is the Grover algorithm, which performs theoretically better than traditional algorithms. This is why there has been research on optimizing it, applying it to new fields, and making it more accessible to industry users. Even if the algorithm is a promising alternative, one of the disadvantages of Grover’s algorithm is the use of an oracle function that must be generated for every set of search data. This review describes three sets of methodologies for generating quantum circuits that can be applied to constructing this oracle quantum circuit.
A Modified ADRC Scheme Based on Model Information for Maglev Train
Authors: Wang Hao , Wang Zhiqiang , Long Zhiqiang
Published: August 2024

During the operation of maglev trains, they are subjected to various disturbances. The presence of these disturbances presents a significant challenge for attaining high-performance control and even poses the risk of system instability. To further enhance the anti-disturbance capability of maglev trains, this paper proposes a model information-assisted modified active disturbance rejection control (MADRC) approach. A mathematical model of the single-point suspension system of maglev trains is constructed for the design of the extended state observer (ESO), which is a modified extended state observer (MESO), and a nonlinear mechanism is incorporated to boost the performance of the ESO. Owing to the introduction of model information, the estimated quantity of disturbances by MESO no longer considers the system model deviation as a disturbance. Hence, the linear feedback control law is modified accordingly. The MESO is regarded as an ESO with time-varying gain using the equivalent gain method, and its stability is proven using the Lyapunov method. The tracking and anti-disturbance performances of different controllers are compared via simulation experiments. Suspension and anti-disturbance experiments are conducted on the single-point suspension experimental platform, verifying that the proposed MADRC has a more potent suppression ability for load disturbances in the suspension system.
Effects of Marigold and Paprika Extracts as Natural Pigments on Laying Hen Productive Performances, Egg Quality and Oxidative Stability
Authors: Matache Cristina-Camelia , Cornescu Gabriela Maria , Drăgotoiu Dumitru , Cișmileanu Ana Elena , Untea Arabela Elena , Sărăcilă Mihaela , Panaite Tatiana Dumitra
Published: August 2024

Enhancing the quality of eggs by using natural food sources has become a very important topic in the last decade. The objective of this study was to determine the influence of natural (marigold and paprika extracts) pigments on the shelf life of eggs from laying hens. This research was carried out for a 6-week period on 168 Lohmann Brown laying hens (45 weeks age) divided into four groups (C, E1, E2 and E3) to assess the performances, external and internal egg quality parameters, egg yolk color, and antioxidant profile. The control group (C) was fed a standard diet (16.39% PB, 2750 kcal EM/kg compound feed) and the experimental diets were supplemented with 0.07% marigold extract (E1), 0.07% paprika extract (E2), and a mixture containing 0.07% of both extracts (E3). In summary, the study demonstrated that adding natural pigments from marigold and paprika extract with highly antioxidant lipid capacity into the diets of laying hens improved egg quality when eggs were stored at 28 days, under both storage temperature conditions (4 °C and 20 °C).
Performance Test of Artificial Defoliating Broccoli Conveyor Line and Analysis of Defoliating Broccoli Inflorescences
Authors: Cao Yunlong , Tang Zhong , Lu Daipeng , Lin Shenbo
Published: August 2024

There is a close relationship between stem and leaf biological characteristics of mature broccoli plants and defoliation technology. Morphological parameters such as the spread degree and diameter of cauliflower stem and leaf and the connection performance of cauliflower stem and leaf were studied. These experiments are helpful to the research of defoliation devices and methods for large-scale manual transportation lines. In this paper, according to the damage forms that may be suffered in the separation process of mature broccoli stems and leaves, “sunny” broccoli varieties were selected. Firstly, the mechanical properties of mature broccoli stems were obtained through different loading methods such as stretching, compression, shearing and bending. Secondly, the stress relaxation characteristics of broccoli were analyzed by static compression of broccoli flower balls. Finally, the separation method of broccoli stems and leaves was simulated by ANSYS. The simulation results showed that shear fracture was more suitable for stem and leaf separation of broccoli than tensile fracture. Then, aiming at the separation of stems and leaves of broccoli, an artificial broccoli assembly line was proposed to ensure the efficiency of leaf removal through man–machine cooperation. The dynamic characteristics of the belt of broccoli leaf removal line were studied to ensure the efficient and stable operation of the conveyor system of broccoli artificial leaf removal line.
Rice Yield and Grain Quality under Fluctuating Soil Moisture Stress
Authors: Deshabandu Kotte Hewa Swarnamali Thilinika , Noda Yasutaka , Marcelo Via Ann Candelaria , Ehara Hiroshi , Inukai Yoshiaki , Kano-Nakata Mana
Published: August 2024

In rainfed lowlands and water-saving cultivation systems, rice plants are often exposed to soil moisture fluctuation (SMF). Improving yield as well as grain quality is the main target for breeding under water-stressed environments. This study investigated the effects of different water treatment on yield, growth parameters, and grain quality under field conditions in Japan for 2 years. Two rice genotypes, Nipponbare (japonica) and G3-3 (derived from Nipponbare and KDML105, indica), were grown under continuous waterlogging (CWL) and SMF conditions. As the grain quality characteristics, grain appearance, dimension, and taste parameters were evaluated as well as yield and yield components. SMF reduced the yield, and G3-3 showed a higher yield than Nipponbare under SMF, which was attributed to the higher number of spikelets per panicle. G3-3 showed a better taste score (mark) with lower protein and amylose contents compared to Nipponbare. However, G3-3 had a higher percentage of broken grains, indicating a trade-off in grain quality traits. Non-structural carbohydrate dynamics may be involved as one of the grain quality characteristics. G3-3 demonstrated a superior yield under SMF conditions and have potential to show superior grain quality, indicating that the introgressed segments of G3-3 may be responsible for the grain quality traits associated with root plasticity.
Characterization of Strubbelig-Receptor Family (SRF) Related to Drought and Heat Stress Tolerance in Upland Cotton (Gossypium hirsutum L.)
Authors: Ahmad Furqan , Rehman Shoaib Ur , Rahman Muhammad Habib Ur , Ahmad Saghir , Khan Zulqurnain
Published: August 2024

Cotton is one of the world’s leading fiber crops, but climate change, drought, heat, and salinity have significantly decreased its production, consequently affecting the textile industries globally. To acclimate to these environmental challenges, a number of gene families involved in various molecular, physiological, and hormonal mechanisms play crucial roles in improving plants response to various abiotic stresses. One such gene family is the GhSRF, a Strubbelig-Receptor family (SRF), and member of the leucine-rich repeat (LRR-V) group. This family encodes leucine-rich repeat transmembrane receptor-like kinases (LRR-RLKs) and have not yet been explored in cotton. Arabidopsis thaliana Strubbelig-Receptor gene sequences were used as queries to identify the homologs in cotton, with subsequent support from the literature and functional prediction through online data. In the current study, a comprehensive genome-wide analysis of cotton was conducted, identifying 22 SRF putative proteins encoded by 22 genes. We performed the detailed analysis of these proteins, including phylogeny, motif and gene structure characterization, promoter analysis, gene mapping on chromosomes, gene duplication events, and chromosomal sub-cellular localization. Expression analysis of putative genes was performed under drought and heat stress conditions using publicly available RNAseq data. The qRT-PCR results showed elevated expression of GhSRF2, GhSRF3, GhSRF4, GhSRF10, and GhSRF22 under drought and heat stress. So, it could be speculated that these genes may play a role in drought and heat tolerance in cotton. These findings could be helpful in cotton breeding programs for the development of climate-resilient cultivars.
Effectiveness of Anaerobic Soil Disinfestation for Weed and Nematode Management in Organic Sweetpotato Production
Authors: Singh Simardeep , Rutter William , Wadl Phillip A. , Campbell Harrison Tyler , Khanal Churamani , Cutulle Matthew
Published: August 2024

Weeds and nematodes are particularly problematic in organic sweetpotato production due to a lack of effective pesticides. Anaerobic soil disinfestation (ASD) has the potential to fit into current pest management practices as an alternative to pesticide application. Greenhouse studies were conducted at the Clemson Coastal Research and Education Center (CREC) in Charleston, SC, to investigate the impact of carbon source amendment and a no carbon source treatment, and soil type on cumulative anaerobicity, weed control, nematode population, and sweetpotato vigor. Microcosms were filled with one of three different soil types (Charleston—loamy/native; Blackville—high coarse sand content; and Clemson—high clay content) and were mixed with cottonseed meal (CSM) or no carbon amendment. The pots were then sealed with plastic totally impenetrable film (Tif) for 6 weeks, followed by the transplanting of sweetpotato (cv Bayou Belle) slips. The results suggested that the CSM-treated microcosms spent more time under anaerobic conditions than those treated with the no carbon amendment. The microcosms that experienced a longer duration of anaerobicity had a lower percent weed cover (49%), fewer nematode egg masses, and a lower gall index when compared to microcosms which experienced a shorter duration of anaerobicity. Significantly higher instances of leaf necrosis were observed in the sweetpotato slips sown in the CSM-treated microcosms. The addition of CSM as a carbon source to facilitate ASD resulted in similar above-ground biomasses of the sweetpotato plants compared to the treatments containing no carbon amendment. However, a significantly lower below-ground biomass of the sweetpotato plants was observed in the CSM-treated microcosms.
Phenotyping for Effects of Drought Levels in Quinoa Using Remote Sensing Tools
Authors: Lupa-Condo Nerio E. , Lope-Ccasa Frans C. , Salazar-Joyo Angel A. , Gutiérrez-Rosales Raymundo O. , Jellen Eric N. , Hansen Neil C. , Anculle-Arenas Alberto , Zeballos Omar , Llasaca-Calizaya Natty Wilma , Mayta-Anco Mayela Elizabeth
Published: August 2024

Drought is a principal limiting factor in the production of agricultural crops; however, quinoa possesses certain adaptive and tolerance factors that make it a potentially valuable crop under drought-stress conditions. Within this context, the objective of the present study was to evaluate morphological and physiological changes in ten quinoa genotypes under three irrigation treatments: normal irrigation, drought-stress followed by recovery irrigation, and terminal drought stress. The experiments were conducted at the UNSA Experimental Farm in Majes, Arequipa, Peru. A series of morphological, physiological, and remote measurements were taken, including plant height, dry biomass, leaf area, stomatal density, relative water content, selection indices, chlorophyll content via SPAD, multispectral imaging, and reflectance measurements via spectroradiometry. The results indicated that there were numerous changes under the conditions of terminal drought stress; the yield variables of total dry biomass, leaf area, and plant height were reduced by 69.86%, 62.69%, and 27.16%, respectively; however, under drought stress with recovery irrigation, these changes were less pronounced with a reduction of 21.10%, 27.43%, and 17.87%, respectively, indicating that some genotypes are adapted or tolerant of both water-limiting conditions (Accession 50, Salcedo INIA and Accession 49). Remote sensing tools such as drones and spectroradiometry generated reliable, rapid, and precise data for monitoring stress and phenotyping quinoa and the optimum timing for collecting these data and predicting yield impacts was from 79–89 days after sowing (NDRE and CREDG r Pearson 0.85).
Reduced Effect of Commercial Leonardite and Seaweed Extract on Lettuce Growth under Mineral, Organic, and No Fertilization Regimes
Authors: Aguiar Peltier , Corrêa Gediane Maria Garcia , Rodrigues Manuel Ângelo , Arrobas Margarida
Published: August 2024

In this study, two commercial products based on the main groups of contemporary biostimulants—a commercial leonardite and a seaweed extract—were tested with the objective of assessing the conditions under which they can enhance lettuce (Lactuca sativa L.) performance, particularly to determine if synergies with conventional fertilization methods can be observed. The experimental protocol was arranged as a factorial design with two factors: organic or mineral fertilization × plant biostimulant. The organic or mineral fertilization factor included five levels: two rates of a nitrogen (N) fertilizer (40 (Nmin40) and 80 (Nmin80) kg ha−1 of N), the same N rates applied as an organic amendment (Norg40 and Norg80), and an unfertilized control (N0). The plant biostimulants used were a commercial leonardite (leonardite) for soil application before planting, a commercial seaweed extract (algae) for foliar application during the growing season, and a control without plant biostimulant. Leonardite significantly increased lettuce dry matter yield (DMY) compared to the control only in the first growing cycle (11.5 and 13.5 g plant−1) and showed no significant interaction with conventional fertilization. It also consistently increased phosphorus (P) levels in the plant tissues. The seaweed extract did not show any effect on the plant, nor did it have any interactions with conventional fertilization regarding DMY. In contrast, with mineral fertilization, lettuce DMY increased from 8.0 and 4.0 g plant−1 (N0) to 22.2 and 12.0 g plant−1 (Nmin80) in the first and second growing cycles, respectively. The response to organic fertilization was lower, yet DMY still increased from 4.0 to 8.1 g plant−1 in the second growing cycle. Generally, this type of plant biostimulant is tested under some form of environmental stress, where it often yields positive results. In this study, the optimal cultivation conditions maintained for the lettuce in the pots likely explain the limited response to the biostimulants. This study suggests that the product labels should more clearly indicate whether they are recommended for general cultivation conditions or specifically for situations where a particular environmental stress can be anticipated.
Automated Assessment of Wheat Leaf Disease Spore Concentration Using a Smart Microscopy Scanning System
Authors: Doroshenko Olga V. , Golub Mikhail V. , Kremneva Oksana Yu. , Shcherban’ Pavel S. , Peklich Andrey S. , Danilov Roman Yu. , Gasiyan Ksenia E. , Ponomarev Artem V. , Lagutin Ilya N. , Moroz Ilya A. , Postovoy Victor K.
Published: August 2024

An advanced approach to the automated assessment of a microscopic slide containing spores is presented. The objective is to develop an intelligent system for the rapid and precise estimation of phytopathogenic spore concentration on microscopic slides, thereby enabling automated processing. The smart microscopy scanning system comprises an electronic microscope, a coordinate table, and software for the control of the coordinate table and image processing. The developed smart microscopy scanning system processes the entire microscope slide with multiple exposed strips, which are automatically determined based on the novel two-stage algorithm. The analysis of trained convolutional neural networks employed for the detection of spore phytopathogens demonstrates high precision and recall metrics. The system is capable of identifying and counting the number of spores of phytopathogenic fungi species Blumeria graminis, Puccinia striiformis, and Pyrenophora tritici-repentis on each exposed strip. A methodology for estimating the spore distribution on a microscopic slide is proposed, which involves calculating the average spore concentration density.
Multi-Year Insights into Industrial Hemp Growth in a Mediterranean Climate
Authors: Tsaliki Eleni , Moysiadis Theodoros , Kalivas Apostolos , Panoras Ioannis , Grigoriadis Ioannis
Published: August 2024

Hemp (Cannabis sativa L.) is a rapidly growing plant with multipurpose uses, and the optimal combination of yield and quality of hemp products (fibers, inflorescences, or seeds) may provide economic opportunities to uncover the full spectrum of its capabilities. The presented experimentation took place over seven years (2017–2023) in Greek climatic conditions for fourteen (14) registered monoecious and dioecious varieties. It can be concluded that the production of biomass, fiber, and seed weight were different not only between varieties but also from year to year. Despite significant variation between harvest years in biomass yield, the extracted fiber was relatively constant. Moreover, not only the dioecious varieties but also the monecious varieties could be effectively grown for biomass production in Greece. Regarding fiber production, monoecious varieties had the highest yields, apart from the dioecious Kompolti variety, which was the most fiber-productive. Under the experimentation conditions, early flowering varieties were most suited for seed production, and the KC Dora variety produced the heavier seeds. Generally, the Futura 75 variety was one of the most productive varieties for biomass and seed weight, while the Bialobrzeskie variety produced the greatest amounts of fiber.
Efficiency of Thyme and Oregano Essential Oils in Counteracting the Hazardous Effects of Malathion in Rats
Authors: Al-Saeed Fatimah A. , Abd-Elghfar Sayed Soliman , Ali Montaser Elsayed
Published: August 2024

The widespread use of MLT may pose numerous hazards to animal breeding, health, and resilience due to the presence of MLT residues in animal feedstuffs, pastures, hay, and cereals. Many medicinal plants provide what is called a generalized anti-toxic remedy. The current study examined hazardous biochemical and histological reactions to MLT and the efficiency of ThEO and OEO essential oils as anti-toxic therapies to return to a natural state after MLT exposure. A total of 75 male albino rats were randomly assigned to two groups: (i) C − MLT, comprising 25 rats, served as the control group; and (ii) C + MLT, with 50 rats that were exposed to 5 mg/kg/BW. After exposure to MLT for 21 days, a return to normal status was determined by subdividing the C + MLT group into two equal groups: ThEO and OEO were used as treatments, with 100 mg/kg body weight of thyme and oregano essential oils, respectively, being administered for 21 days. The results showed a significant decrease in body weight gain (BWG) and final weight (FW) compared to C − MLT, while the therapeutic effects of ThEO and OEO enhanced FW and BWG. Our results indicated that MLT exposure resulted in deficient serum liver function, but that OEO and ThEO therapy brought about a significant improvement in liver enzyme function. Although there was no significant difference in serum aspartate transaminase (AST) or alkaline phosphatase (ALK-Ph) and a significant drop in alanine transaminase (ALT) and acetyl choline-esterase (AChE) levels, the C + MLT group showed hepatic fibrosis in the third stage. Furthermore, histological sections of the OEO and ThEO groups showed reduced hepatocellular damage, inflammation, and hepatic fibrosis. However, there was a significant increase in serum creatinine between the C + MLT and C − MLT groups following exposure to MLT. Histological sections of renal tissue from rats treated with OEO and ThEO showed reduced tubular damage, reduced interstitial inflammation, and preserved renal tissue architecture. In conclusion, OEO and ThEO are potential compounds for use as anti-toxic therapies to return to a natural state after MLT exposure. These compounds could serve as an experimental therapeutic approach against natural toxins, providing a solution to the problems of raising livestock that are exposed to nutritional toxicity.
Equine Nuclear Medicine in 2024: Use and Value of Scintigraphy and PET in Equine Lameness Diagnosis
Authors: Spriet Mathieu , Vandenberghe Filip
Published: August 2024

Scintigraphy and Positron Emission Tomography (PET) are both nuclear medicine imaging techniques, providing functional information of the imaged areas. Scintigraphy is a two-dimensional projected imaging technique that was introduced in equine imaging in the late 1970s. Scintigraphy allows imaging of large body parts and can cover multiple areas, remaining the only technique commonly used in horses for whole body imaging. PET is a cross-sectional imaging technique, first used in horses in 2015, allowing higher resolution three-dimensional functional imaging of the equine distal limb. This manuscript will cover current use and values of these two modalities in equine lameness diagnosis.
Buserelin Acetate Added to Boar Semen Enhances Litter Size in Gilts in Tropical Environments
Authors: Taechamaeteekul Preechaphon , Jaijarim Chatchapong , Audban Chairach , Sang-Gassanee Kridtasak , Numsri Pongsak , Lin Hongyao , Collell Miquel , Tummaruk Padet
Published: August 2024

The use of exogenous hormones has long been of interest for improving reproductive performance in swine production. Enhancing litter size directly impacts the economic efficiency of pig production. Various strategies, including nutritional, genetic, and hormonal approaches, have been explored with varying degrees of success. Administering a gonadotropin-releasing hormone (GnRH) agonist, such as buserelin, at the onset of estrus can induce ovulation and reduce the variation in ovulation timing among sows. This study assessed the impact of GnRH agonist supplementation in boar semen doses on the litter size of inseminated gilts. The research was conducted on a commercial swine herd in northern Thailand. A total of 231 Landrace × Yorkshire crossbred gilts, aged 224.5 ± 16.2 days at the onset of estrus synchronization, participated in the experiment. The gilts’ estrus was synchronized with oral altrenogest supplementation at a dosage of 20 mg/day for 18 days. After exhibiting standing estrus, the gilts were randomly divided into three groups. Control group: gilts were inseminated at 0 and 12 h post standing estrus onset with a conventional semen dose (n = 94). Treatment 1: similar to the control group, but with an added 5 µg (1.25 mL) of buserelin acetate to the boar semen dose during the first insemination (n = 71). Treatment 2: similar to the control group, but with 10 µg (2.5 mL) of buserelin acetate added to the boar semen dose during the first insemination (n = 66). All gilts were inseminated twice during their standing estrus using the intrauterine artificial insemination method. Each semen dose contained 3.0 × 109 motile sperm in 80 mL. The farrowing rate averaged 78.8% and did not significantly differ between the groups (p = 0.141). The total number of piglets born per litter in the treatment 2 group was greater than in the control group (14.0 ± 0.3 vs. 13.2 ± 0.3, respectively, p = 0.049), but was not significantly different from the treatment 1 group (13.3 ± 0.3, p = 0.154). Similarly, the number of live-born piglets in the treatment 2 group was greater than in the control and treatment 1 groups (13.2 ± 0.4 vs. 12.3 ± 0.3 and 12.0 ± 0.4, respectively, p < 0.05). Moreover, the live-born piglets’ litter birth weight in the treatment 2 group was greater than in the control group (17.0 ± 0.4 vs. 15.6 ± 0.3 kg, respectively, p = 0.008) and the treatment 1 group (15.7 ± 0.4 kg, p = 0.025). In conclusion, adding a GnRH agonist to boar semen appears to enhance the litter size of gilts. Further research should focus on understanding the underlying mechanisms and determining the optimal dose and timing for GnRH agonist supplementation.
Characterization of Post-Mortem pH Evolution and Rigor Mortis Process in Botucatu Rabbit Carcasses of Different Categories
Authors: Dutra Daniel Rodrigues , Villegas-Cayllahua Erick Alonso , Baptista Giovanna Garcia , Ferreira Lucas Emannuel , Castilha Leandro Dalcin , Borba Hirasilva
Published: August 2024

The aim of the present study was to evaluate the characteristics of carcasses, monitor their pH evolution during the first 24 h post-mortem, and determine the time required for the establishment and resolution of rigor mortis in different categories of Botucatu rabbits. Live weight at slaughter, carcass weight, and yield were higher in 12-month-old animals compared to 3-month-old rabbits, regardless of sex. There was an effect of muscle type, age, and sex on the kinetics of muscle acidification, with the Biceps femoris showing a significantly higher pH than the Longissimus lumborum from 4 h post-mortem onward. The establishment of rigor mortis occurred at 5 h post-mortem in young rabbits and bucks and at 6 h post-mortem in does, along with pH stabilization, while the resolution of rigor occurred at 18 h post-mortem for all types of carcasses evaluated. In conclusion, Botucatu rabbit carcasses should be chilled continuously at 4 °C for a minimum of 18 h to ensure efficient rigor mortis progression and muscle-to-meat transformation throughout the carcass.
RETRACTED: Teklemariam et al. Isolation and Characterization of a Novel Lytic Phage, vB_PseuP-SA22, and Its Efficacy against Carbapenem-Resistant Pseudomonas aeruginosa. Antibiotics 2023, 12, 497
Authors: Teklemariam Addisu D. , Al-Hindi Rashad R. , Alharbi Mona G. , Alotibi Ibrahim , Azhari Sheren A. , Qadri Ishtiaq , Alamri Turki , Esmael Ahmed , Harakeh Steve
Published: August 2024

The journal retracts the article, “Isolation and Characterization of a Novel Lytic Phage, vB_PseuP-SA22, and Its Efficacy against Carbapenem-Resistant Pseudomonas aeruginosa”, cited above [...]
Origami of KR-12 Designed Antimicrobial Peptides and Their Potential Applications
Authors: Lakshmaiah Narayana Jayaram , Mechesso Abraham Fikru , Rather Imran Ibni Gani , Zarena D. , Luo Jinghui , Xie Jingwei , Wang Guangshun
Published: August 2024

This review describes the discovery, structure, activity, engineered constructs, and applications of KR-12, the smallest antibacterial peptide of human cathelicidin LL-37, the production of which can be induced under sunlight or by vitamin D. It is a moonlighting peptide that shows both antimicrobial and immune-regulatory effects. Compared to LL-37, KR-12 is extremely appealing due to its small size, lack of toxicity, and narrow-spectrum antimicrobial activity. Consequently, various KR-12 peptides have been engineered to tune peptide activity and stability via amino acid substitution, end capping, hybridization, conjugation, sidechain stapling, and backbone macrocyclization. We also mention recently discovered peptides KR-8 and RIK-10 that are shorter than KR-12. Nano-formulation provides an avenue to targeted delivery, controlled release, and increased bioavailability. In addition, KR-12 has been covalently immobilized on biomaterials/medical implants to prevent biofilm formation. These constructs with enhanced potency and stability are demonstrated to eradicate drug-resistant pathogens, disrupt preformed biofilms, neutralize endotoxins, and regulate host immune responses. Also highlighted are the safety and efficacy of these peptides in various topical and systemic animal models. Finaly, we summarize the achievements and discuss future developments of KR-12 peptides as cosmetic preservatives, novel antibiotics, anti-inflammatory peptides, and microbiota-restoring agents.
High and Low Dosage of Vancomycin in Polymethylmethacrylate Cements: Efficacy and Mechanical Properties
Authors: Kwong Jeffrey W. , Abramowicz Michael , Kühn Klaus Dieter , Foelsch Christian , Hansen Erik N.
Published: August 2024

Introduction: Prosthetic joint infections (PJIs) are difficult to treat and represent a significant burden to the healthcare system. Two-stage revision surgery with placement of an antibiotic-loaded cement spacer is currently the gold standard for treatment in the United States for late-onset infections. We evaluate the efficacy of varying doses of vancomycin added to antibiotic-containing acrylic cement spacers and discuss the biomechanical and antimicrobial properties of using high versus low doses of vancomycin in cement spacers in the hip and knee. Materials and Methods: Commercially available Copal cement containing either gentamicin and clindamycin (G + C) or gentamicin and vancomycin (G + V) was prepared with the manual addition of low (2 g) and high (6 g) doses of vancomycin. In vitro mechanical testing was then carried out according to ISO 5833 and DIN 53435, as well as inhibition zone assays against common PJI pathogens. Additionally, inhibition zone assays were conducted on two commercially available prefabricated spacers containing gentamicin: Copal Exchange G and Cemex Spacer-K. Results: In biomechanical testing, Copal G + V with the addition of 6 g of vancomycin failed to meet the ISO standard. Copal G + C and Copal G + V with low and high dosages of vancomycin were all effective against the tested pathogens and displayed constant efficacy for a duration of 42 days. High doses of vancomycin showed significantly lower mechanical stability. Moreover, Copal Exchange G showed significantly larger inhibition zones across 42 days. Discussion: While higher concentrations of vancomycin appear to improve the antimicrobial efficacy of cement, they also reduce its mechanical stability. Despite its smoother surface, the Copal Exchange G spacer exhibits large inhibition zones after 1 day and maintains consistently large inhibition zones over 6 weeks. Thus, it may be preferred for use in two-stage revision surgery. Conclusion: Copal Exchange G is more effective than Cemex Spacer K against S. aureus and E. coli. The manual addition of vancomycin to cement containing double antibiotics is very effective. The influence on ISO compression is low, the ISO bending modulus is increased, and ISO bending, DIN bending, and DIN impact, are reduced.
The Effects of Maca (Lepidium meyenii Walp) on Cellular Oxidative Stress: A Systematic Review and Meta-Analysis
Authors: Huerta Ojeda Álvaro , Rodríguez Rojas Javiera , Cuevas Guíñez Jorge , Ciriza Velásquez Stephanie , Cancino-López Jorge , Barahona-Fuentes Guillermo , Yeomans-Cabrera María-Mercedes , Pavez Leonardo , Jorquera-Aguilera Carlos
Published: August 2024

Lepidium meyenii Walp (LmW) or Maca, including its bioactive components such as macamides, among others, has demonstrated antioxidant effects. However, the effect size (ES) of LmW on oxidative stress has not been qualitatively described and calculated. The primary objective of this systematic review and meta-analysis was to review and qualitatively describe the studies published up to 2023 that supplemented LmW to control cellular oxidative stress; the secondary objective was to calculate the ES of the different interventions. The search was designed following the PRISMA® guidelines for systematic reviews and meta-analyses and performed in the Web of Science, Scopus, SPORTDiscus, PubMed, and MEDLINE until 2023. The selection of studies included randomized controlled trials, with tests and post-tests, both in vitro and in vivo in animals and humans. The methodological quality and risk of bias were evaluated with the CAMARADES tool. The main variables were reduced glutathione, glutathione peroxidase, superoxide dismutase, and malondialdehyde. The analysis was conducted with a pooled standardized mean difference (SMD) through Hedges’ g test (95% CI). Eleven studies were included in the systematic review and eight in the meta-analysis. They revealed a small effect for reduced glutathione (SMD = 0.89), a large effect for glutathione peroxidase (SMD = 0.96), a moderate effect for superoxide dismutase (SMD = 0.68), and a moderate effect for malondialdehyde (SMD = −0.53). According to the results, the phytochemical compounds of LmW effectively controlled cellular oxidative stress, mainly macamides. It was also determined that a higher dose of LmW generated a greater antioxidant effect. However, information concerning humans is scarce.
Extracellular Vesicle-Enclosed Oxidative Stress- and Inflammation-Related microRNAs as Potential Biomarkers of Vitamin D Responsivity: A Pilot Study on Inflammatory Bowel Disease Patients with or without COVID-19
Authors: Ammirata Giorgia , Arigoni Maddalena , Licastro Danilo , Caviglia Gian Paolo , Disabato Michela , Zubair Ghania , Bezzio Cristina , Saibeni Simone , De Nicolò Amedeo , Cusato Jessica , Palermiti Alice , Manca Alessandra , Tolosano Emanuela , Cozzini Stefano , Mancini Marcello , Altruda Fiorella , D’Avolio Antonio , Ribaldone Davide Giuseppe , Ala Ugo , Fagoonee Sharmila
Published: August 2024

The relationship between serum 25-hydroxyvitamin D (25(OH)D) levels, genomic response to vitamin D (Vit.D), and positivity to SARS-CoV-2 remains understudied. In this pilot study, during the follow-up of patients with Inflammatory Bowel Disease (IBD) and COVID-19, we investigated this issue by analyzing the molecular contents of serum extracellular vesicles (EVs) from six groups of IBD patients (n = 32), classified according to anti-SARS-CoV-2 status, 25(OH)D level, and Vit.D supplementation, by small RNA-seq. This analysis revealed differentially expressed miRNAs, PIWI-RNA, transfer RNA, small nucleolar RNAs, and protein-coding RNAs in the EVs obtained from these cohorts of IBD patients. Experimental validation evidenced a statistically significant increase in miR30d-5p, miR150-5p, Let-7f-5p, and Let-7a-5p in the anti-SARS-CoV-2-positive and low 25(OH)D and Vit.D supplemented groups with respect to the non-Vit.D supplemented group, indicating their responsiveness to Vit.D treatment. Bioinformatics analysis highlighted the regulation of these validated miRNAs by oxidative stress and inflammation, hallmarks of IBD and COVID-19. Our study reports an unprecedented panel of circulating EV-enclosed inflammation- and oxidative stress-related miRNAs, the potentiality of which, as biomarkers for Vit.D responsivity in IBD patients, needs to be explored in future studies on larger cohorts in order to allow clinicians to optimize current treatment strategies upon viral infection.
Multi-Body Dynamics Modeling and Simulation of Maglev Satellites
Authors: Li Zongyu , Wang Weijie , Wang Lifen
Published: August 2024

The Lorentz force magnetic levitation gim2bal stabilized platform (LFMP), as a new generation of high-precision turntable for maglev satellites, can meet the requirements of future spacecraft for ultra-high attitude pointing accuracy and stability. To solve the problem of three-module multi-body attitude control under maneuvering conditions, the platform subsystem is first dynamically modeled based on the second type of Lagrangian equation, and the payload subsystem is dynamically modeled based on the Newton–Euler method. Secondly, a multi-loop control system is designed, consisting of high-precision and fast attitude pointing control for the payload, position tracking control for the platform subsystem, and tracking control for the maglev module. The final simulation results verified the feasibility and effectiveness of the payload-centered control method. An evaluation of the stability with a specific model has been performed, and the attitude accuracy of the payload is within 0.00002° and the attitude stability is within 0.00005°/s.
Analysis of the Stability of a High Fill Slope under Different Gradients and Precipitation Conditions
Authors: Zhang Hongda , Zhang Chengda , Zheng Weiqiang , Wang Xiaoquan , Zhang Jiangwei
Published: August 2024

The stability problem of high fill slopes has always been a research hotspot. Its failure mechanism is complex and prominent, featuring strong concealment, a short occurrence time and great harmfulness. In this paper, the stability of a high fill slope under rainfall conditions will be studied by using indoor tests, numerical simulations, etc. The study is based on a high fill slope in Yichang City. The evolution law of high fill slope stability under the maximum rainfall condition is revealed. The results show the following: The influence of moisture content on stress–strain curves is reflected in both the curve’s shape and the peak value of deviatoric stress. Under the constraint of confining pressure, the curve decreases and the peak value of deviatoric stress decreases with the increase of moisture content at the same confining pressure. The safety factor obtained by a rigid body limit equilibrium analysis and numerical calculation indicates that the safety factor for a 30° slope meets the requirements for slope stability evaluation and remains in a fundamentally stable state. An on-site investigation suggests that surface failure and shallow failure may be primary failure modes for this slope; therefore, it is recommended to implement slope protection measures. This study provides valuable references for similar high fill slopes.