bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of the Detection and Ligation of Patent Processus Vaginalis Between Laparoscopy-Assisted Transscrotal Orchiopexy and Single Scrotal Incision Orchiopexy
Authors: Zhao Weiguang , Su Cheng , Li Shoulin , Mo Zengnan
Published: January 2022

This study aimed to compare the detection and ligation of patent processus vaginalis (PPV) between laparoscopy-assisted transscrotal orchidopexy (LATO) and single scrotal incision orchiopexy (SSIO) for low palpable undescended testis (UDT). We performed a retrospective medical record review of transscrotal orchidopexies performed for low palpable UDT at our institution from 2017 to 2019; 33 and 39 boys underwent LATO and SSIO, respectively. Data collection included patient demographics, incidence of PPV, operative time, and clinical outcomes. All 95 testes were delivered into the scrotum. There was no significant difference between the two groups with respect to patients' age, side, and mean operative time. The incidence of PPV in the LATO group was significantly higher than that in the SSIO group (56.52 vs. 34.69%, P = 0.04). The incidence of contralateral PPV in the LATO group was 45%. One patient in the SSIO group underwent unilateral PV ligation and laparoscopic exploration revealed bilateral PPV owing to metachronous contralateral hydrocele. One patient in the LATO group demonstrated obliterated PV in the initial transscrotal procedure, but an ipsilateral PPV was found in the latter laparoscopic procedure. In conclusion, LATO has a higher detection rate and higher ligation of the PPV than SSIO, suggesting that, LATO may help reduce recurrent PPV-related issues. However, long-term follow-up results are needed to evaluate the advantages and disadvantages in a larger case series.
Climate Conditions During a Rift Valley Fever Post-epizootic Period in Free State, South Africa, 2014–2019
Authors: Anyamba Assaf , Damoah Richard , Kemp Alan , Small Jennifer L. , Rostal Melinda K. , Bagge Whitney , Cordel Claudia , Brand Robert , Karesh William B. , Paweska Janusz T.
Published: January 2022

Rift Valley fever virus (RVFV) activity in Southern Africa tends to occur during periods of sustained elevated rainfall, cooler than normal conditions, and abundant vegetation cover creating ideal conditions for the increase and propagation of populations of RVFV mosquito vectors. These climatic and ecological conditions are modulated by large-scale tropical-wide El Niño–Southern Oscillation (ENSO) phenomena. The aim of this 5-year study was to investigate climatic conditions during Rift Valley fever “post-epizootic” period in Free State province of the Republic of South Africa, which historically experienced the largest RVF outbreaks in this country. We collected satellite-derived rainfall, land surface temperature (LST), and normalized difference vegetation index (NDVI) data since 2014 to understand broad environmental conditions in the years following a period of sustained and widespread large RVF outbreaks (2008–2011) in the region. We found this post-epizootic/interepizootic period to be characterized by below-normal rainfall (~-500 mm), above LSTs (~+12°C), depressed NDVI (60% below normal), and severe drought as manifested particularly during the 2015–2016 growing season. Such conditions reduce the patchwork of appropriate habitats available for emergence of RVFV vectors and diminish chances of RVFV activity. However, the 2016–2017 growing season saw a marked return to somewhat wetter conditions without any reported RVFV transmission. In general, the aggregate vector collections during this 5-year period follow patterns observed in climate measurements. During the 2017–2018 growing season, late and seasonally above average rainfall resulted in a focal RVF outbreak in one location in the study region. This unanticipated event is an indicator of cryptic RVF activity during post-epizootic period and may be a harbinger of RVFV activity in the coming years.
Studies on the Possibilities of Processing Buckwheat Husks and Ash in the Production of Environmentally Friendly Fertilizers
Authors: Pocienė Odeta , Šlinkšienė Rasa
Published: January 2022

The sustainable utilization of different food waste and other products is one of the challenges of the European Green Course. Buckwheat has major potential as a food ingredient; however, processing buckwheat into food products generates a large amount of solid waste that needs to be sustainably disposed of. The by-products that come from the processing of the buckwheat contain high contents of carbon and hydrogen and can be used as raw materials for the production of granular biofuels. This work proposes and explores the potential of a different route of buckwheat husk ash utilization. Chemical analysis of the buckwheat husk ash (BHA) and uncleaned buckwheat husks (UBH) showed significant amounts of primary and secondary nutrients (0.28 ± 0.06%–5.84 ± 0.43% P2O5; 4.56 ± 0.46%–38.63 ± 1.82% K2O; 0.09 ± 0.01%–12.18 ± 0.38% CaO and 0.47 ± 0.08%–3.56 ± 0.18% MgO) as well as micronutrients (Zn, Mn, Cu, and Fe) and carbon (29.53 ± 0.50%–54.35 ± 0.58% C). It has been determined that granular biofertilizers can be produced by using drum granulators from the mixture of raw materials in an 80–20% BHA, 20–80% UBH, and 10% polyvinyl acetate (PVA) solution. However, when more than 20% of the UBH is present in the raw material mixture, the humidity of granules is high (more than 6%), and bulk density is low (less than 450 kg/m3). The pH values of 10% solution of the produced granules range from 12.0 to 9.7; thus, the fertilizers can act as a liming agent, which can be recommended for acid soils. This suggests a potential for a cradle-to-cradle type of regenerative engineering process design, where the end product of buckwheat processing—buckwheat husk ash and untreated buckwheat husks—can be returned back to the soil to replenish the nutrients, resulting in an overall sustainable process.
Development of a Three-Dimensional Plant Localization Technique for Automatic Differentiation of Soybean from Intra-Row Weeds
Authors: Su Wen-Hao , Sheng Ji , Huang Qing-Yang
Published: January 2022

Soybean is a legume that is grown worldwide for its edible bean. Intra-row weeds greatly hinder the normal growth of soybeans. The continuous emergence of herbicide-resistant weeds and the increasing labor costs of weed control are affecting the profitability of growers. The existing cultivation technology cannot control the weeds in the crop row which are highly competitive with the soybean in early growth stages. There is an urgent need to develop an automated weeding technology for intra-row weed control. The prerequisite for performing weeding operations is to accurately determine the plant location in the field. The purpose of this study is to develop a plant localization technique based on systemic crop signalling to automatically detect the appearance of soybean. Rhodamine B (Rh-B) is a signalling compound with a unique fluorescent appearance. Different concentrations of Rh-B were applied to soybean based on seed treatment for various durations prior to planting. The potential impact of Rh-B on seedling growth in the outdoor environment was evaluated. Both 60 and 120 ppm of Rh-B were safe for soybean plants. Higher doses of Rh-B resulted in greater absorption. A three-dimensional plant localization algorithm was developed by analyzing the fluorescence images of multiple views of plants. The soybean location was successfully determined with the accuracy of 97%. The Rh-B in soybean plants successfully created a machine-sensible signal that can be used to enhance weed/crop differentiation, which is helpful for performing automatic weeding tasks in weeders.
Development Changes in the Physicochemical Composition and Mineral Profile of Red-Fleshed Dragon Fruit Grown under Semi-Arid Conditions
Authors: Singh Akath , Swami Saurabh , Panwar Nav Raten , Kumar Mahesh , Shukla Anil Kumar , Rouphael Youssef , Sabatino Leo , Kumar Pradeep
Published: January 2022

Red-fleshed dragon fruit offers an array of bioactive compounds. Its cultivation is gaining momentum in India, including in arid and semi-arid conditions. Among various factors, the fruit developmental stages and climatic conditions of locations greatly influence the biosynthesis and actual contents of different bioactive compounds and mineral contents of the fruit. The changes in physical attributes, and bioactive and mineral content in red-fleshed dragon fruit grown in semi-arid conditions in India were assessed and quantified at six developmental stages. Significant changes occurred in the physical attributes of the fruit and the bioactive compounds and mineral content during the fruit maturation stages of the red-fleshed dragon fruit. The fruit physical characteristics, such as fruit size, fruit and pulp weight gain, peel thickness, and fruit firmness, along with eating quality parameters, such as soluble solids, sugars, acidity and soluble proteins, were observed at optimum at 35 days after anthesis (DAA). The decrease in total phenolics (29.96%), total flavonoids (41.06%), and vitamin C (75.3%) occurred throughout the fruit development stages, whereas the content of betalains, which was detected initially at 25 DAA, increased (48.6%) with the progression of the fruit development stages. However, the antioxidant capacity and free radical scavenging activity demonstrated variable trends throughout the fruit maturation period. There was an increasing trend in all the minerals up to 35 days, followed by a slight decrease, except for phosphorus content, which increased until the last stage of evaluation. The colour characteristics, in conjunction with the bioactive and antioxidant potential determined in the present study, suggest that red-fleshed dragon fruit can be harvested at 35 DAA for long-distance transportation, and from 35 to 40 DAA for local marketing.
A Comparative Study of Ensemble Models for Predicting Road Traffic Congestion
Authors: Bokaba Tebogo , Doorsamy Wesley , Paul Babu Sena
Published: January 2022

Increased road traffic congestion is due to different factors, such as population and economic growth, in different cities globally. On the other hand, many households afford personal vehicles, contributing to the high volume of cars. The primary purpose of this study is to perform a comparative analysis of ensemble methods using road traffic congestion data. Ensemble methods are capable of enhancing the performance of weak classifiers. The comparative analysis was conducted using a real-world dataset and bagging, boosting, stacking and random forest ensemble models to compare the predictive performance of the methods. The ensemble prediction models are developed to predict road traffic congestion. The models are evaluated using the following performance metrics: accuracy, precision, recall, f1-score, and the misclassification cost viewed as a penalty for errors incurred during the classification process. The combination of AdaBoost with decision trees exhibited the best performance in terms of all performance metrics. Additionally, the results showed that the variables that included travel time, traffic volume, and average speed helped predict vehicle traffic flow on the roads. Thus, the model was developed to benefit transport planners, researchers, and transport stakeholders to allocate resources accordingly. Furthermore, adopting this model would benefit commuters and businesses in tandem with other interventions proffered by the transport authorities.
A Fuzzy Method for Joint Resource Allocation and Stable Pairing in D2D Communications
Authors: Pourmoslemi Alireza , Rajabi Siavash , Salimi Mehdi , Ferrara Massimiliano
Published: January 2022

In this paper, a device-to-device (D2D) communications multiple-metric scenario for resource allocation and pairing is studied. To improve the D2D connection stability, a fuzzy method based on the data rate and battery levels of potential D2D pairs is proposed in such a way that the set of D2D transmitters is considered as a fuzzy set. A single cell scenario with a cellular user and some co-channel D2D nodes consists of D2D receivers, and transmitters are taken into account. Furthermore, a stable fuzzy pairing criterion is proposed for the selection of the best D2D transmitter. The proposed method is compared with three other pairing methods named the maximum sum rate, constant pairing method, and random pairing method. The simulation results show that the mentioned pairing method outperforms the other three methods in terms of stability and fairness criteria and follows the maximum sum-rate method from the sum-rate criteria point of view.
Analysis of Factors Affecting Field Applicability and Long-Term Performance Analysis of LCP Woven Geotextile for Soft Ground Reinforcement
Authors: Yan Yu , Hahm Wangyu , Kim Seunghyun , Youk Jiho , Jeon Hanyong
Published: January 2022

In recent years, natural disasters have been increasing worldwide due to rapid climate change, and the damage to ground structures is increasing due to the destruction of the ground. Damage to the ground structure can be reduced or eliminated by using LCP woven geotextiles as ground reinforcement. Therefore, in this study, the tensile properties, reduction factor affecting long-term performance, creep behavior, and fatigue properties of LCP woven geotextile were tested and analyzed. As a result, in the case of tensile properties, the maximum tensile strength of the LCP woven geotextile was 192.94 kN/m2 in the MD direction, and it was generally constructed so that the load was transmitted. The total reduction factor is 1.86, which could be applied within 53.8% of the design strength when applied to the field. In addition, it was considered that the effect of the reduction factor for creep deformation on the long-term performance was dominant. Through the analysis of the creep behavior and fatigue characteristics, considering that the creep limit strain was 10%, if an earthquake occurred after 50 years of construction, it can be predicted that up to 90% of UTS would exhibit seismic performance. When LCP woven geotextile was applied as reinforcement, if the cyclic load due to fatigue failure was less than 478,000 times per year, it was considered that there was little possibility of the collapse of the ground structure.
A Comparison of the Validity of Three Exercise Tests for Estimating Maximal Oxygen Uptake in Korean Adults Aged 19–64 Years
Authors: Chung Jinwook , Lee Kihyuk
Published: January 2022

The purpose of this study was to compare the validity of three submaximal exercise tests (SMETs) and develop practical predictive models for the VO2max in Korean adults. A total of 541 (287 males and 254 females) adults participated in this study. Their ages ranged from 19 to 64. The VO2max was measured using the maximal-graded exercise treadmill test. The SMETs were performed by a treadmill test, the YMCA step test, and the PACER test. Regression analysis was conducted to compare the validity of the VO2max predictive equations using SMETs. The validity of the predictive models was evaluated using explanatory power, standard error of estimate (SEE), and Bland-Altman analysis. The explanatory power between the measured VO2max and the predicted VO2max was 58.0% (<0.001), 59.2% (<0.001), and 71.7% (<0.001), respectively. The SEEs were 4.545, 4.478, and 3.732 (mL/kg/min). The models were significant predictors of VO2max and had acceptable validity in a large sample of Korean adults. Especially, among the predictive models, PACER had the highest acceptable effectiveness. Therefore, the equations developed in this study are recommended to better evaluate the cardiovascular endurance of Korean adults.
Selection of a Sustainable Structural Beam Material for Rural Housing in Latin América by Multicriteria Decision Methods Means
Authors: Nicolalde Juan Francisco , Yaselga John , Martínez-Gómez Javier
Published: January 2022

Latin America presents the need of more accessible housing in the rural areas of the region; however, problems that this populations have to face are cost, accessibility and seismic events that leave structural damages in the buildings of the population, raising the need of resisting permanent housing or temporary shelter. Moreover, the conventional steel materials are not always affordable, which makes us consider the utilization of alternative sustainable materials that are easier to reach and with a seismic resistance. In this sense, the conditions of an earthquake are simulated, specialized software selects and recreates candidate materials and multicriteria decision methods make the final selection of the best material to be evaluated in a simulation. In this way, it has been found that a beam must resist an effort of 5.96 MPa, candidate materials are composites and naturals, and considering mechanical, environmental and price aspects, the decision methods chose the bamboo as the best material. Lastly, another simulation validates the selection, allowing us to conclude that the multicriteria decision methods used succeeded to find the best alternative.
Dynamic Lateral Response of the Partially-Embedded Single Piles in Layered Soil
Authors: Ma Jianjun , Han Shujuan , Gao Xiaojuan , Li Da , Guo Ying , Liu Qijian
Published: January 2022

Scouring can reduce the strength and rigidity of the pile–soil system and become one of the major causes for the failure of the structure of the partially-embedded single pile. The stratification of the soil fields has a significant influence on the internal force and deformation of laterally-loaded piles. A dynamic model of the laterally-loaded single pile in layered soil is established employing Hamilton’s principle based on the modified Vlasov foundation model. Then, the finite difference method is used to obtain the numerical matrix containing the control equations of the single pile to achieve accurate modeling of the soil–structure interaction (SSI) system affected by scouring, so as to solve the natural frequencies of the single pile. Green’s function method obtains the analytical solution of the forced vibration of the single pile. The effects of scouring and the layered soil on the dynamic response of the single pile are studied by numerical calculation and parameter analysis. It is shown that the dynamic model of the partially-embedded single pile in layered soil based on the modified Vlasov foundation model can accurately predict the dynamic characteristics of pile foundation affected by scouring. As the scouring degree intensifies, the first-order natural frequencies of the single pile in layered soil decrease significantly. The subgrade reaction coefficient of each layer of soil in the modified Vlasov foundation model decreases, and the shear coefficient increases. The first-order natural frequencies of the single pile at each scour level increase with the increase in the thickness of the underlying soil. When the elastic modulus of the first layer of soil is increased by one time, the first-order natural frequencies of the single pile are increased by about 20%.
Demountable Bolted Shear Connector for Easy Deconstruction and Reconstruction of Concrete Slabs in Steel–Concrete Bridges
Authors: Jung Dae-Sung , Park Se-Hyun , Kim Tae-Hyeong , Han Jong-Wook , Kim Chul-Young
Published: January 2022

Welded headed studs are used to connect steel girders and concrete slabs in steel composite bridges. However, environmental problems related to welded shear connectors have been reported, including noise and dust generation during the replacement and demolition of damaged or worn concrete slabs. To overcome these issues, demountable shear connectors that enable easy replacement of damaged or worn concrete slabs must be developed. However, conventional bolted shear connectors mainly use embedded nuts, which cannot be used in structures such as bridges because initial slip occurs and the stiffness and shear resistance strength are relatively low. To address these issues, this paper proposes a bolted shear connector that integrates the embedded nut part into the stud bolt and has a tapered shape at the bottom of the expansion column. For performance verification in regard to static shear strength and slip displacement, push-out tests were performed according to Eurocode-4, and the performance of the proposed stud bolt was compared to those of conventional welded headed studs and embedded nut-style bolted shear connectors. In addition, an analytical review was performed using the ABAQUS program for the 1/4 axisymmetric model of the push-out test. As a result of applying the material nonlinear model and the surface contact analysis, the load-slip displacement of each analysis model showed a tendency extremely similar to the experimental results. The proposed demountable bolted shear connector exhibited excellent shear performance and can be used as a satisfactory replacement for conventional welded studs because it meets slip displacement and ductility standards.
Chain Graph Explanation of Neural Network Based on Feature-Level Class Confusion
Authors: Hwang Hyekyoung , Park Eunbyung , Shin Jitae
Published: January 2022

Despite increasing interest in developing interpretable machine learning methods, most recent studies have provided explanations only for single instances, require additional datasets, and are sensitive to hyperparameters. This paper proposes a confusion graph that reveals model weaknesses by constructing a confusion dictionary. Unlike other methods, which focus on the performance variation caused by single-neuron suppression, it defines the role of each neuron in two different perspectives: ‘correction’ and ‘violation’. Furthermore, our method can identify the class relationships in similar positions at the feature level, which can suggest improvements to the model. Finally, the proposed graph construction is model-agnostic and does not require additional data or tedious hyperparameter tuning. Experimental results show that the information loss from omitting the channels guided by the proposed graph can result in huge performance degradation, from 91% to 33%, while the proposed graph only retains 1% of total neurons.
A PROMETHEE Multiple-Criteria Approach to Combined Seismic and Flood Risk Assessment at the Regional Scale
Authors: Soldati Arianna , Chiozzi Andrea , Nikolić Željana , Vaccaro Carmela , Benvenuti Elena
Published: January 2022

Social vulnerability is deeply affected by the increase in hazardous events such as earthquakes and floods. Such hazards have the potential to greatly affect communities, including in developed countries. Governments and stakeholders must adopt suitable risk reduction strategies. This study is aimed at proposing a qualitative multi-hazard risk analysis methodology in the case of combined seismic and flood risk using PROMETHEE, a Multiple-Criteria Decision Analysis technique. The present case study is a multi-hazard risk assessment of the Ferrara province (Italy). The proposed approach is an original and flexible methodology to qualitatively prioritize urban centers affected by multi-hazard risks at the regional scale. It delivers a useful tool to stakeholders involved in the processes of hazard management and disaster mitigation.
Magnetic Field Action on Limnospira indica PCC8005 Cultures: Enhancement of Biomass Yield and Protein Content
Authors: Deamici Kricelle Mosquera , de Morais Michele Greque , dos Santos Lucielen Oliveira , Gros Fabrice , Costa Jorge Alberto Vieira , Laroche Céline
Published: January 2022

The effects of a magnetic field (MF) on the cyanobacteria Limnospira indica PCC 8005 growth rate and biomass composition were investigated. A device to apply the MF during the cultivation was built and the cyanobacteria were exposed to a steady 11 mT transverse MF. The growth increased with MF application, and when it was applied for 1 h per day, 123% more biomass was produced than in the control group. The protein content in the biomass cultured under this condition increased, achieving 60.4 w/w, while the Chl-a increased by 326%. The MF application for 1 h per day was found to be more efficient than when applied continuously for 24 h per day, in addition to being more economical and sustainable. This study showed an inexpensive and non-toxic way to enhance biomass concentration, leading to amounts more than 100% higher than those obtained in the control group. Furthermore, the high protein content in the biomass gave us several possibilities to increase the nutritional value of food.
Dynamic Discrete Inventory Control Model with Deterministic and Stochastic Demand in Pharmaceutical Distribution
Authors: Antic Slobodan , Djordjevic Milutinovic Lena , Lisec Andrej
Published: January 2022

This paper presents an inventory control problem in a private pharmaceutical distribution company from the Republic of Serbia. The company realizes that distribution within nine neighbouring countries and inventory control in the pharmaceutical supply chain is centralized. In order to constitute a conceptual model of the problem, we propose the modern control theory concept. The conceptual model is based on the specific practical assumptions and constraints of the supply chain. Thereafter, a dynamic discrete mathematical model of inventory control is formulated to reflect elements of the system and their relations. The model considers multiple pharmaceutical products, variable lead time, realized stochastics and deterministic demand, and different ordering policies (Lot for Lot and Fixed Order Quantity). Deterministic demand is represented as a sales forecast for each product per month, while stochastic demand is generated as a random variation of sales forecast in a range of ±20%. Two objective functions are defined as the maximization of the difference between planned average inventory level and realized average inventory level, and the minimization of stock-out situations. We develop a procedure for the determination of reorder points and the number of deliveries to achieve proposed objective functions. The model overcomes shortages of theoretically-based distribution requirements planning models and offers solutions to the limitations in inventory control practice. Real-life data, collected over two years, are used for the validation of the proposed model and the solution procedure. Numerical examples illustrate the model application and behaviour.
Smart Energy Management System: Design of a Monitoring and Peak Load Forecasting System for an Experimental Open-Pit Mine
Authors: Laayati Oussama , Bouzi Mostafa , Chebak Ahmed
Published: January 2022

Digitization in the mining industry and machine learning applications have improved the production by showing insights in different components. Energy consumption is one of the key components to improve the industry’s performance in a smart way that requires a very low investment. This study represents a new hardware, software, and data processing infrastructure for open-pit mines to overcome the energy 4.0 transition and digital transformation. The main goal of this infrastructure is adding an artificial intelligence layer to energy use in an experimental open-pit mine and giving insights on energy consumption and electrical grid quality. The achievement of these goals will ease the decision-making stage for maintenance and energy managers according to ISO 50001 standards. In order to minimize the energy consumption, which impact directly the profit and the efficiency of the industry, a design of a monitoring and peak load forecasting system was proposed and tested on the experimental open-pit mine of Benguerir. The main challenges of the application were the monitoring of typical loads machines per stage, feeding the supervisors by real time energy data on the same process SCADA view, parallel integrating hardware solutions to the same process control system, proposing a fast forest quantile regression algorithm to predict the energy demand response based on the data of different historical scenarios, finding correlations between the KPIs of energy consumption, mine production process and giving global insights on the electrical grid quality.
Compounding Effects of Fluvial Flooding and Storm Tides on Coastal Flooding Risk in the Coastal-Estuarine Region of Southeastern China
Authors: Lu Weiwei , Tang Lihua , Yang Dawen , Wu Heng , Liu Zhiwu
Published: January 2022

In coastal areas of southeastern China, multiple flood drivers such as river flow, precipitation and coastal water level can lead to compound flooding which is often much greater than flooding simulated by one flood driver in isolation. Bivariate probability distributions accounting for compound flooding from river discharge and sea level were constructed based on MvCAT (Multivariate Copula Analysis Toolbox) combined with goodness of fit tests in 15 coastal-estuarine regions of Southeastern China. Flood typing-based bivariate probability distributions considering multiple flood-generating mechanisms were also built. Our results indicated that the performance of flood typing-based bivariate distribution was not significantly better than the bivariate probability distribution in coastal-estuarine regions based on the Mann–Whitney U test; the compounding effects of river discharge and sea level had limited impact on bivariate return periods, but had greater impact on coastal flooding risk in terms of design values. Ignoring compounding effects of river discharge and sea level leads to significant underestimation of design values. The results suggest that the compounding effect of river discharge and sea level should be considered when calculating design values in coastal flood risk assessment.
Analysis of the Environmental Impact in the Life Cycle of a Single-Family House in Poland
Authors: Grygierek Krzysztof , Ferdyn-Grygierek Joanna
Published: January 2022

Buildings consume energy, which is a major contributor to greenhouse gas emissions. In order to reduce their environmental impact, it is important to choose suitable building materials and energy source. The paper presents the results of a multi-variant analysis of five environmental impact categories (global warming potential, acidification, eutrophication, ozone layer depletion, and total use of primary energy) for a single-family house located in Poland in a 25-year life cycle. The tests were carried out for two different types of building structures (brick and wooden), two variants of external partition insulation, and four heat sources. The following computer programs were used for the calculations: OpenStudio and EnergyPlus, and One Click LCA. The analysis took into account the erection of the building in its shell and the energy consumption for heating in a 25-year life cycle. Studies have shown that when an electric heat source is used, the use stage has the greatest environmental impact, accounting for up to 80% of the total emissions over the life cycle. This ratio can be reduced by half in the case of a house with thicker external wall insulation and an eco-biomass boiler.
Increasing the Efficiency of Canola and Soybean GMO Detection and Quantification Using Multiplex Droplet Digital PCR
Authors: Demeke Tigst , Lee Sung-Jong , Eng Monika
Published: January 2022

The number of genetically modified (GM) events for canola, maize, and soybean has been steadily increasing. Real-time PCR is widely used for the detection and quantification of individual GM events. Digital PCR (dPCR) has also been used for absolute quantification of GM events. A duplex dPCR assay consisting of one reference gene and one GM event has been carried out in most cases. The detection of more than one GM event in a single assay will increase the efficiency of dPCR. The feasibility of detection and quantification of two, three, and four GM canola and soybean events at the same time was investigated at 0.1%, 1%, and 5% levels using the QX200 Droplet Digital PCR (ddPCR) system. The reference gene assay was carried out on the same plate but in different wells. For some of the assays, optimization of the probe concentrations and labels was needed for successful ddPCR. Results close to the expected result were achieved for duplex, triplex, and tetraplex ddPCR assays for GM canola events. Similar ddPCR results were also achieved for some GM soybean events with some exceptions. Overall, absolute quantification of up to four GM events at the same time improves the efficiency of GM detection.
Using Pen-Side Measurable Blood Parameters to Predict or Identify Dystocic Lambing Events
Authors: Redfearn Amellia , McNally Jody , Brewer Heather , Doyle Emma , Schmoelzl Sabine
Published: January 2022

Dystocia is the greatest contributor to neonatal lamb mortality in Australia and poses significant welfare and economic concerns worldwide. In this study, we set out to investigate whether pen-side analysis technology could be employed to detect blood parameters predictive of dystocic labour events in sheep. In a pilot trial, we collected and analysed blood samples in pen-side assays for glucose, lactate, pH, pCO2, pO2, base excess, HCO3, TCO2, sO2, lactate, sodium, potassium, chloride, calcium, urea nitrogen, creatinine, haematocrit, haemoglobin and anion gap. From the pilot data, we identified creatinine, TCO2, chloride and calcium as potentially useful markers. To develop a time course and to establish variability of the selected blood parameters, a time series of samples was collected from 12 ewes, from mid-gestation to 48 h after birth. For the main trial, blood samples were collected at mid- and late gestation for glucose determination and for the full set of blood parameters at three time points before, at and after birth. Possible predictors of lambing difficulty were chloride, haematocrit and haemoglobin, sampled one week before birth; creatinine, sampled at birth; and blood pH and base excess after birth. In conclusion, we found that pen-side analysis of blood markers showed promise in identifying dystocic lambing events.
MOET Efficiency in a Spanish Herd of Japanese Black Heifers and Analysis of Environmental and Metabolic Determinants
Authors: Vázquez-Mosquera Juan M. , Fernández-Novo Aitor , Bonet-Bo Martin , Pérez-Villalobos Natividad , Pesántez-Pacheco Jose L. , Pérez-Solana Maria Luz , de Mercado Eduardo , Gardón Juan Carlos , Villagrá Arantxa , Sebastián Francisco , Pérez-Garnelo Sonia Salomé , Martínez Daniel , Astiz Susana
Published: January 2022

Multiple ovulation and embryo transfer (MOET) systems have been intensively implemented in Japanese Black cattle in Japan and to create Japanese Black herds out of these areas. Environmental conditions influence MOET efficiency. Thus, we describe results of 137 in vivo, non-surgical embryo flushings performed between 2016–2020, in a full-blood Japanese Black herd kept in Spain and the possible effects of heat, year, bull, donor genetic value, and metabolic condition. Additionally, 687 embryo transfers were studied for conception rate (CR) and recipient related factors. A total of 71.3% of viable embryos (724/1015) were obtained (5.3 ± 4.34/flushing). Donor metabolites did not affect embryo production (p > 0.1), although metabolite differences were observed over the years, and by flushing order, probably related to the donor age. CR was not affected by embryo type (fresh vs. frozen), recipient breed, and whether suckling or not suckling (p > 0.1). CR decreased significantly with heat (44.3 vs. 49.2%; (p = 0.042)) and numerically increased with recipient parity and ET-number. Pregnant recipients showed significantly higher levels of cholesterol-related metabolites, glucose, and urea (p < 0.05). Therefore, adequate MOET efficiency can be achieved under these conditions, and heat stress should be strongly avoided during Japanese Black embryo transfers. Moreover, recipients’ metabolites are important to achieve pregnancy, being probably related to better nutrient availability during pregnancy.
Isolation of Endogenous TGF-β1 from Root Canals for Pulp Tissue Engineering: A Translational Study
Authors: Widbiller Matthias , Rosendahl Andreas , Wölflick Melanie , Linnebank Moritz , Welzenbach Benedikt , Hiller Karl-Anton , Buchalla Wolfgang , Galler Kerstin
Published: January 2022

Cell homing for dental pulp tissue engineering has been advocated as a feasible approach to regenerate dental pulp in a clinical setting. In order to develop a translational protocol for clinical application, we wanted to determine the effects of disinfectants on the availability of growth factors from the root canal, the amount that can be obtained in this context, and whether they can be processed for use in tissue engineering procedures. The extraction of growth factors should also be confirmed in a clinical setting. Root canals were prepared in 36 extracted mature teeth, and the amount of TGF-β1 in solution was quantified after different irrigation protocols (sodium hypochlorite, chlorhexidine) and after intracanal medication (calcium hydroxide). Centrifugal filters with a cut-off of 10,000 Da and 3000 Da were used for efficient concentration, and volumes and amounts of retained TGF-β1 were measured at different time points. During conventional endodontic treatment, ethylenediaminotetraacetic acid (EDTA) solution was collected after ultrasonic activation from the root canals of mature teeth of 38 patients, and growth factor content was quantified via enzyme-linked immunosorbent assay (ELISA). Irrigation with sodium hypochlorite reduced TGF-β1 release into EDTA. This effect was partially reversed by canal enlargement after the use of sodium hypochlorite and by subsequent use of calcium hydroxide. A few minutes of centrifugation with a cut-off of 10,000 Da reduced the initial volume of the irrigant by 90% and led to a continuous increase in concentration to the same extent. Furthermore, TGF-β1 was obtained from root canals of mature teeth during endodontic treatment in quantities that have been shown to elicit desirable cellular responses in a subsequent clinical application. A mixture with a suitable scaffold material and injection into the root canal has the potential to promote dental pulp regeneration.
Resident Self-Tissue of Proinflammatory Cytokines Rather Than Their Systemic Levels Correlates with Development of Myelofibrosis in Gata1low Mice
Authors: Zingariello Maria , Verachi Paola , Gobbo Francesca , Martelli Fabrizio , Falchi Mario , Mazzarini Maria , Valeri Mauro , Sarli Giuseppe , Marinaccio Christian , Melo-Cardenas Johanna , Crispino John D. , Migliaccio Anna Rita
Published: January 2022

Serum levels of inflammatory cytokines are currently investigated as prognosis markers in myelofibrosis, the most severe Philadelphia-negative myeloproliferative neoplasm. We tested this hypothesis in the Gata1low model of myelofibrosis. Gata1low mice, and age-matched wild-type littermates, were analyzed before and after disease onset. We assessed cytokine serum levels by Luminex-bead-assay and ELISA, frequency and cytokine content of stromal cells by flow cytometry, and immunohistochemistry and bone marrow (BM) localization of GFP-tagged hematopoietic stem cells (HSC) by confocal microscopy. Differences in serum levels of 32 inflammatory-cytokines between prefibrotic and fibrotic Gata1low mice and their wild-type littermates were modest. However, BM from fibrotic Gata1low mice contained higher levels of lipocalin-2, CXCL1, and TGF-β1 than wild-type BM. Although frequencies of endothelial cells, mesenchymal cells, osteoblasts, and megakaryocytes were higher than normal in Gata1low BM, the cells which expressed these cytokines the most were malignant megakaryocytes. This increased bioavailability of proinflammatory cytokines was associated with altered HSC localization: Gata1low HSC were localized in the femur diaphysis in areas surrounded by microvessels, neo-bones, and megakaryocytes, while wild-type HSC were localized in the femur epiphysis around adipocytes. In conclusion, bioavailability of inflammatory cytokines in BM, rather than blood levels, possibly by reshaping the HSC niche, correlates with myelofibrosis in Gata1low mice.
Paper-Based Electrodes Conjugated with Tungsten Disulfide Nanostructure and Aptamer for Impedimetric Detection of Listeria monocytogenes
Authors: Mishra Annu , Pilloton Roberto , Jain Swati , Roy Souradeep , Khanuja Manika , Mathur Ashish , Narang Jagriti
Published: January 2022

In this study, we report on a novel aptasensor based on an electrochemical paper-based analytical device (ePAD) that employs a tungsten disulfide (WS2)/aptamer hybrid for the detection of Listeria monocytogenes. Listeria is a well-known causative pathogen for foodborne diseases. The proposed aptasensor signifies many lucrative features which include simple, cost-effective, reliable, and disposable. Furthermore, the use of an aptamer added more advantageous features in the biosensor. The morphological, optical, elemental composition, and phase properties of the synthesized tungsten disulfide (WS2) nanostructures were characterized by field-emission scanning electron microscopy (FESEM), RAMAN spectroscopy, photoluminescence (PL), and X-ray diffraction (XRD), while electrochemical impedance spectroscopy was performed to corroborate the immobilization of aptamer and to assess the L. monocytogenes sensing performance. The limit of detection (LoD) and limit of quantification (LoQ) of the aptasensor was found to be 10 and 4.5 CFU/mL, respectively, within a linear range of 101–108 CFU/mL. The proposed sensor was found to be selective solely towards Listeria monocytogenes in the presence of various bacterial species such as Escherichia coli and Bacillus subtilis. Validation of the aptasensor operation was also evaluated in real samples by spiking them with fixed concentrations (101, 103, and 105) of Listeria monocytogenes, thereby, paving the way for its potential in a point-of-care scenario.
Hacking Commensal Bacteria to Consolidate the Adaptive Mucosal Immune Response in the Gut–Lung Axis: Future Possibilities for SARS-CoV-2 Protection
Authors: Pereira Marcela , Oh Ju Kyoung , Kang Dae-Kyung , Engstrand Lars , Valeriano Valerie Diane
Published: January 2022

Infectious diseases caused by mucosal pathogens significantly increase mortality and morbidity. Thus, the possibility to target these pathogens at their primary entry points can consolidate protective immunity. Regarding SARS-CoV-2 infection, it has been observed that the upper respiratory mucosa is highly affected and that dysregulation of resident microbiota in the gut–lung axis plays a crucial role in determining symptom severity. Thus, understanding the possibility of eliciting various mucosal and adaptive immune responses allows us to effectively design bacterial mucosal vaccine vectors. Such design requires rationally selecting resident bacterial candidates as potential host carriers, evaluating effective carrier proteins for stimulating an immune response, and combining these two to improve antigenic display and immunogenicity. This review investigated mucosal vaccine vectors from 2015 to present, where a few have started to utilize Salmonella and lactic acid bacteria (LAB) to display SARS-CoV-2 Spike S proteins or fragments. Although current literature is still lacking for its studies beyond in vitro or in vivo efficiency, decades of research into these vectors show promising results. Here, we discuss the mucosal immune systems focusing on the gut–lung axis microbiome and offer new insight into the potential use of alpha streptococci in the upper respiratory tract as a vaccine carrier.
Comprehension of the Co-Operation of Professional Identity and Metacognition of English Teachers in Pedagogical Problem Solving
Authors: Han Insuk
Published: January 2022

Teaching is a metacognitive pedagogical problem-solving process. Teachers’ commitment to this process can be partly influenced by their professional identity (PI) in the pursuit of identity-congruent actions and identity verification. For these pursuits, teachers produce cognitive, affective, and behavioural responses, which are the representations of PI, and use metacognition by monitoring and regulating these responses for successful pedagogical problem solving. Teachers, in turn, improve their PI and metacognition. Based on a narrative approach, the problem-solving processes of five Korean teachers of English are explored along with their PI and metacognition operation. This exploration provides the rationales for the conceptualised co-operation mechanism of teacher PI and metacognition, and also reveals the possibility of its variation. Comprehension of the mechanism enables teacher educators and policy makers to establish specific plans and procedures for principled professional development or policy support. Thus, based on the conceptualisation and findings, systematic interventions via problem-solving-based teacher education and contextual support, which help teachers develop PI and metacognition, are discussed.
Risk Stratification of Thyroid Nodules: From Ultrasound Features to TIRADS
Authors: Rago Teresa , Vitti Paolo
Published: January 2022

Thyroid nodules are common in iodine deficient areas, in females, and in patients undergoing neck irradiation. High-resolution ultrasonography (US) is important for detecting and evaluating thyroid nodules. US is used to determine the size and features of thyroid nodules, as well as the presence of neck lymph node metastasis. It also facilitates guided fine-needle aspiration (US-FNA). The most consistent US malignancy features of thyroid nodules are spiculated margins, microcalcifications, a taller-than-wide shape, and marked hypoechogenicity. Increased nodular vascularization is not identified as a predictor of malignancy. Thyroid elastosonography (USE) is also used to characterize thyroid nodules. In fact, a low elasticity of nodules at USE has been related to a higher risk of malignancy. According to their US features, thyroid nodules can be stratified into three categories: low-, intermediate-, and high-risk nodules. US-FNA is suggested for intermediate and high-risk nodules.
Simultaneous Resection of Pancreatic Neuroendocrine Tumors with Synchronous Liver Metastases: Safety and Oncological Efficacy
Authors: Addeo Pietro , Cusumano Caterina , Goichot Bernard , Guerra Martina , Faitot François , Imperiale Alessio , Bachellier Philippe
Published: January 2022

Whether the simultaneous resection of pancreatic neuroendocrine tumors (PNET) with synchronous liver metastases (LM) is safe and oncologically efficacious remains to be debated. We retrospectively reviewed clinical data from patients who underwent the simultaneous resection of PNETs with LMs over the last 25 years. Fifty-one consecutive patients with a median age of 54 years (range 27–80 years) underwent pancreaticoduodenectomy (PD) (n = 16), distal pancreatosplenectomy (DSP) (n = 32) or total pancreatectomy (n = 3) with synchronous LM resection. There were no differences in the postoperative outcomes in term of mortality (p = 0.33) and morbidity (p = 0.76) between PD and DSP. The median overall survival (OS) was 64.78 months (95% CI: 49.7–119.8), and the overall survival rates at 1, 3, and 5 years were 97.9%, 86.2% and 61%, respectively. The OS varied according to the tumor grade (G): G1 (OS 128 months, 5-year OS 83%) vs. G2 (OS 60.5 months, 5-year OS 58%) vs. G3 (OS 49.7 months, 5-year OS 0%) (p = 0.03). Multivariate Cox analysis identified G as the only prognostic factor (HR: 5.56; 95% CI: 0.91–9.60; p = 0.01). Simultaneous PNETS with LMs can be performed safely with acceptable morbidity and mortality at tertiary centers. Well-differentiated PNETs had longer survival and might benefit the most from these extended surgeries.