bluuebunny/crossref_metadata_2025 huggingface dataset:
The Dynamics of Sustainable Material Selection for Green-Certified Projects
Authors: Ismaeel Walaa S. E.
Published: August 2023

Material selection is a challenging process in which several parameters should be considered for green-certified projects. Hence, this study investigates the dynamics of sustainable material selection (SMS) across the project life cycle using system thinking. A dynamic model was developed based on the Leadership in Energy and Environmental Design where relevant environmental categories and credits were represented, and their dependency coefficients (DCs) were computed. This pinpointed the effect of SMS on the point-accrual pattern across project phases, showing that the high leverage points were demonstrated for the operation phase. The result showed that SMS extended beyond the efficient use of materials, which indicated a DC of 100% in the Materials and Resources category to affect other sustainable categories, i.e., the indoor environmental quality, energy efficiency, and sustainability of the project site comprised 68.8%, 57.6%, and 50% of DCs, respectively. Thus, optimal SMS could earn the project 48% of the total possible points, putting it in the ‘Certified’ level. Accordingly, the proposed model can be used to evaluate the level of certification as well as the building performance therein.
Development and Performance Evaluation of UHPC and HPC Using Eco-Friendly Additions as Substitute Cementitious Materials with Low Cost
Authors: Abdul Sahib Mohammed Qusay , Farzam Masood , Sukkar Khalid A.
Published: August 2023

Ultra-high-performance concrete (UHPC) and high-performance concrete (HPC) are widely used in construction engineering applications. The quality and economy of this type of concrete are the main challenges in real construction systems due to their expensive cost. In the present investigation, the performances of UHPC and HPC were improved using eco-friendly additives from natural sources or industrial wastes. Accordingly, different kinds of concrete mixtures were prepared with the addition of various eco-friendly materials, such as metakaolin (10, 15, and 20%), silica fume (2.5, 5, 10, and 15%), cement kiln dust (CKD) (0, 5, and 10%), and 1 vol.% of steel and polypropylene fibers. All of these materials were subjected to efficient treatment and purification processes. The results indicated that the prepared UHPC was characterized by high compression and flexural strengths. The prepared UHPC (sample CR-2) with metakaolin (10%), CKD (10%), and 1 vol.% of steel fibers provided the highest compressive strength of 135 MPa at 28 days. Moreover, the results showed that reducing the cement amounts to 750, 500, and 250 kg/m3 provided concrete with efficient structural requirements and specifications and can be characterized as UHPC and HPC. Also, the mixture (sample CM15) with a metakaolin addition of 15%, CKD of 100 kg/m3, and 1 vol.% of steel fibers showed the highest flexural strength of 19.14 MPa at 28 d. Moreover, the highest splitting tensile strength of the prepared UHPC cylinders was 9.6 MPa at 28 d for the MSS1000 sample, which consisted of 15% metakaolin, a cement content of 1000 kg/m3, silica fume of 10%, and steel fibers of 1% vol. The prepared UHPC mixtures will reduce the amount of consumed cement and the production cost, with a high performance in comparison to classical concrete. Finally, it was clear that the prepared UHPC and HPC concrete with green additions can serve efficiently in specific construction applications, with high performance, economic feasibility, and safe environmental impacts.
Where to Draw the LINE—Are Retrotransposable Elements Here to Stay?
Authors: Bergin Christopher J. , Mendes da Silva Amanda , Benoit Yannick D.
Published: August 2023

The frequency of somatic retrotranspositions of Long Interspersed Nuclear Elements 1 (LINE1) over a lifetime in healthy colonic epithelium and colorectal tumors has recently been reported. Indicative of a cell type-specific effect, LINE1 sequences in colonic epithelium showed lower levels of DNA methylation compared to other cell types examined in the study. Consistent with a role for DNA methylation in transposon silencing, the decreases in DNA methylation observed at LINE1 elements in colonic epithelium were accompanied by increases in LINE1 mRNA levels. In human primary colorectal tumors, LINE1 retrotransposition frequency was tenfold higher than in normal colonic tissues, with insertions potentially altering genomic stability and cellular functions. Here, we discuss the discoveries made by Nam and colleagues, emphasizing the intestinal-specific methylation signature regulating the LINE1 lifecycle and how this new information could shape future drug discovery endeavors against colorectal cancer.
Conventional DNA-Damaging Cancer Therapies and Emerging cGAS-STING Activation: A Review and Perspectives Regarding Immunotherapeutic Potential
Authors: Lewicky Jordan D. , Martel Alexandrine L. , Gupta Mukul Raj , Roy René , Rodriguez Galaxia M. , Vanderhyden Barbara C. , Le Hoang-Thanh
Published: August 2023

Many traditional cancer treatments such as radiation and chemotherapy are known to induce cellular DNA damage as part of their cytotoxic activity. The cGAS-STING signaling axis, a key member of the DNA damage response that acts as a sensor of foreign or aberrant cytosolic DNA, is helping to rationalize the DNA-damaging activity of these treatments and their emerging immunostimulatory capacity. Moreover, cGAS-STING, which is attracting considerable attention for its ability to promote antitumor immune responses, may fundamentally be able to address many of the barriers limiting the success of cancer immunotherapy strategies, including the immunosuppressive tumor microenvironment. Herein, we review the traditional cancer therapies that have been linked with cGAS-STING activation, highlighting their targets with respect to their role and function in the DNA damage response. As part of the review, an emerging “chemoimmunotherapy” concept whereby DNA-damaging agents are used for the indirect activation of STING is discussed as an alternative to the direct molecular agonism strategies that are in development, but have yet to achieve clinical approval. The potential of this approach to address some of the inherent and emerging limitations of cGAS-STING signaling in cancer immunotherapy is also discussed. Ultimately, it is becoming clear that in order to successfully employ the immunotherapeutic potential of the cGAS-STING axis, a balance between its contrasting antitumor and protumor/inflammatory activities will need to be achieved.
Efficient Conversion of Monosaccharides into 5-Hydroxymethylfurfural Using Acidic Deep Eutectic Solvents
Authors: To Linh Ngoc , Nguyen Trinh Hao , Nguyen Thien Phuoc , Phan Ha Bich , Nguyen Linh Ho Thuy , Doan Tan Le Hoang , Dang Chinh Van , Tran Phuong Hoang
Published: August 2023

In this study, a quick, simple, green method of converting carbohydrates into 5-hydroxymethylfurfural (HMF) with the use of deep eutectic solvents (DESs) was reported on. We synthesized 12 DESs for HMF conversion from carbohydrates which were studied under different conditions. Under optimal conditions, oxalic acid and citric acid with a choline chloride-based DES produced a maximum yield of HMF at 59 ± 2% and 62 ± 3% in 5 min at 120 °C, respectively. The efficiency of converting glucose to HMF in a short time (5 min) at 140 °C using CrCl3 with a choline chloride-based DES was around 37 ± 1%, which was higher than in previous work. This study demonstrates the significant potential of DESs as a combination for the continuous catalytic transformation of biomass in the synthesis of platform chemicals.
Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development
Authors: Kamaletdinova Tatiana , Zong Wen , Urbánek Pavel , Wang Sijia , Sannai Mara , Grigaravičius Paulius , Sun Wenli , Fanaei-Kahrani Zahra , Mangerich Aswin , Hottiger Michael O. , Li Tangliang , Wang Zhao-Qi
Published: August 2023

Poly(ADP-ribose) polymerase-1 (PARP1) binds DNA lesions to catalyse poly(ADP-ribosyl)ation (PARylation) using NAD+ as a substrate. PARP1 plays multiple roles in cellular activities, including DNA repair, transcription, cell death, and chromatin remodelling. However, whether these functions are governed by the enzymatic activity or scaffolding function of PARP1 remains elusive. In this study, we inactivated in mice the enzymatic activity of PARP1 by truncating its C-terminus that is essential for ART catalysis (PARP1ΔC/ΔC, designated as PARP1-ΔC). The mutation caused embryonic lethality between embryonic day E8.5 and E13.5, in stark contrast to PARP1 complete knockout (PARP1−/−) mice, which are viable. Embryonic stem (ES) cell lines can be derived from PARP1ΔC/ΔC blastocysts, and these mutant ES cells can differentiate into all three germ layers, yet, with a high degree of cystic structures, indicating defects in epithelial cells. Intriguingly, PARP1-ΔC protein is expressed at very low levels compared to its full-length counterpart, suggesting a selective advantage for cell survival. Noticeably, PARP2 is particularly elevated and permanently present at the chromatin in PARP1-ΔC cells, indicating an engagement of PARP2 by non-enzymatic PARP1 protein at the chromatin. Surprisingly, the introduction of PARP1-ΔC mutation in adult mice did not impair their viability; yet, these mutant mice are hypersensitive to alkylating agents, similar to PARP1−/− mutant mice. Our study demonstrates that the catalytically inactive mutant of PARP1 causes the developmental block, plausibly involving PARP2 trapping.
The Concept of Moving School and Its Practical Implementation in Bavarian Higher Secondary Schools
Authors: Englert Paul , Andrä Christian , Demetriou Yolanda
Published: August 2023

The roles of physical activity and the reduction in sedentary activities in the healthy physical, psychosocial and mental development of children and adolescents are undisputed. This is where the concept of Moving School comes in, which has been expanded to a holistic approach that takes into account all areas of the school, including lessons, after-school care and breaks, and aims to provide students with a sufficient level of physical activity. There are no current studies that show to what extent this concept has arrived in the theoretical specifications for higher secondary schools and to what extent it is implemented in practice. In this study, by conducting a narrative review, we determine the core elements of the concept of Moving School. Furthermore, we analyse the extent to which these elements of Moving School are taken into account in the school curriculum, in published school programmes and on school homepages. In this study, we revealed that the concept of Moving School has hardly been implemented in practice in Bavarian higher secondary schools as mostly only single elements of it are referred to in the school curriculum, programmes and school homepages. It can therefore be assumed that the concept has not yet been able to achieve its intended effect, namely, to fill the daily lives of schoolchildren with movement, play and sport.
Studies on Protective Coatings for Molding Tools Applied in a Precision Glass Molding Process for a High Abbe Number Glass S-FPM3
Authors: Chen Chong , Friedrichs Marcel , Jiang Cheng , Wang Li-Ang , Dang Ming-Yang , Grunwald Tim , Bergs Thomas , Li Yong-Liang
Published: August 2023

Precision glass molding (PGM) is an efficient process used for manufacturing high-precision micro lenses with aspheric surfaces, which are key components in high-resolution systems, such as endoscopes. In PGM, production costs are significantly influenced by the lifetimes of elaborately manufactured molding tools. Protective coatings are applied to the molding tools to withstand severe cyclic thermochemical and thermomechanical loads in the PGM process and, in this way, extend the life of the molding tools. This research focuses on a new method which combines metallographic analysis and finite element method (FEM) simulation to study the interaction of three protective coatings—diamond-like carbon (DLC), PtIr and CrAlN—each in contact with the high Abbe number glass material S-FPM3 in a precision glass molding process. Molding tools are analyzed metallographically using light microscopy, white light interferometry, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). The results show that the DLC coating improved process durability more than the PtIr and CrAlN coatings, in which the phenomenon of coating delamination and glass adhesion can be observed. To identify potential explanations for the metrological results, FEM is applied to inspect the stress state and stress distribution in the molding tools during the molding process.
Uniform-Density Micro-Textured Ball-End Milling Cutter Model Based on Fractal and Uniform Distribution Theory
Authors: Han Pei , Tong Xin , Yang Shucai , Wang Xiyue
Published: August 2023

At present, for micro-textured tools, the determination of the micro-texture placement area depends on the derivation of the cutting geometric model. The micro-texture distribution form applies geometric methods, and the research methods and accuracy are limited. Therefore, in this paper, the ball-end milling cutter is taken as the research object. Based on fractal theory, the morphology of the tool before and after wear is compared to determine the tool–chip contact area. The uniform-density micro-texture distribution model is established using the uniform distribution point theorem, and the synergistic mechanism of the edge and the micro-texture is revealed. The strength of the micro-textured tool with uniform density under the action of the edge is studied by simulation. Finally, the determination of the tool–chip contact area and the establishment of a uniform-density micro-texture model is realized. It is proved that the synergistic effect of the cutting edge and the micro-texture has a positive effect on the milling behavior of the tool. When comparing the non-edge and non-texture tools with the cutting-edge tools, the maximum strain and stress of the cutting-edge micro-textured tools increased by 12% and 30%, and 30% and 20%, respectively, without affecting the normal use of the tool. This research provides a new method for the design of micro-textured tools.
Characterization of Reactive Sputtered Chromium Oxynitride Coatings Developed on Glass Substrate
Authors: Rawal Sushant , Chauhan Kamlesh V. , Patel Nicky P.
Published: August 2023

In this study, we investigate how changing the nitrogen flow rate, the length of time during deposition, and the intensity of pressure have an impact on the resulting chromium oxynitride coatings. Depending on the sputtering conditions, the X-ray diffraction analyses reveal different textures in the Cr2O3 and Cr2N phases. Films deposited with varying nitrogen flow rates and deposition durations experience compressive strains, whereas films produced with varying sputtering pressures witness tensile stresses. Film surface energies and contact angles were measured with a contact angle goniometer. Because of their hydrophobic properties, chromium oxynitride coatings may find use as water-repellent, self-cleaning surfaces. Chromium oxynitride films’ absorption and transmission curves were recorded using a UV-Vis-NIR spectrophotometer. The band gap of chromium oxynitride coatings reduces with a rise in the flow of nitrogen and sputtering time but widens with increasing deposition pressure.
Novel Cellular and Immunotherapy: Toxicities and Perioperative Implications
Authors: De Camilli Alessandro , Fischer Gregory
Published: August 2023

Targeted cellular and immunotherapies have welcomed a new chapter in multi-modal cancer therapy. These agents harness our innate immune system and destroy malignant cells in a precise way as compared with “legacy” chemotherapeutic agents that largely rely on abolishing cell division. New therapies can augment the T-cell recognition of tumor antigens and effectively prevent tumor cells from their historically successful ability to evade immune recognition. These novel agents cause acute and chronic toxicities to a variety of organ systems (enteritis, pneumonitis, hypophysitis, and hepatitis), and this may masquerade as other chronic illnesses or paraneoplastic effects. As the perioperative footprint of cancer patients increases, it is essential that perioperative providers—anesthesiologists, surgeons, nurse anesthetists, and inpatient hospital medicine providers—be up to date on the physiologic mechanisms that underlie these new therapies as well as their acute and subacute toxicity profiles. Immunotherapy toxicity can significantly impact perioperative morbidity as well as influence perioperative management, such as prophylaxis for adrenal insufficiency, preoperative pulmonary assessment, and screening for thyroid dysfunction, among others.
Cost Drivers and Financial Burden for Cancer-Affected Families in China: A Systematic Review
Authors: Jia Yufei , Jiang Weixi , Yang Bolu , Tang Shenglan , Long Qian
Published: August 2023

This systematic review examined cancer care costs, the financial burden for patients, and their economic coping strategies in mainland China. We included 38 quantitative studies that reported out-of-pocket payment for cancer care and patients’ coping strategies in English or Chinese (PROSPERO: CRD42021273989). We searched PubMed, Embase, Ovid, Web of Science, Cochrane, CNKI, and Wanfang Data from 1 January 2009 to 10 August 2022. We referred to the standards for reporting observational studies to assess the methodological quality and transparent reporting of the included studies and reported the costs narratively. Annual mean medical costs (including inpatient and outpatient costs and fees for self-purchasing drugs) ranged from USD 7421 to USD 10,297 per patient. One study investigated medical costs for 5 years and indicated that inpatient costs accounted for 51.6% of the total medical costs, followed by self-purchasing drugs (43.9%). Annual medical costs as a percentage of annual household income ranged from 36.0% to 63.1% with a metaproportion of 51.0%. The common coping strategies included borrowing money and reduction of household expenses and expenses from basic health services. Costs of inpatient care and self-purchasing drugs are major drivers of medical costs for cancer care, and many affected households shoulder a very heavy financial burden.
Sustainability at Home: The Development of an Efficient Framework for Home Energy-Saving Applications
Authors: Tongsubanan Sarunporn , Kasemsarn Kittichai
Published: August 2023

Energy consumption is increasing due to the rise in the world population, industrialization, and urbanization, particularly in the residential sector, attributed to a lack of user-friendly tools. This study seeks to create a research framework and wireframe for home energy-saving applications. A systematic literature review (SLR) was conducted using the VOSviewer software version 1.6.18 tool to pinpoint the research problems. Three key research problems were identified: Inadequate information presentation for both experts and non-experts, insufficient consideration for middle-aged and elderly users, and difficulties in interpreting graphics or images on the application’s display screens. This qualitative research involved three rounds of co-creation activities with nine experts and nine non-experts to identify major problems and preliminary solutions. As a result, two key issues were addressed from the qualitative data: The problem of area calculation, resolved by simplifying data entry processes, and the issue of material selection within homes, improved by incorporating illustrative images with concise, easily understandable descriptions. The outcome of this research is a framework and wireframe that lays the groundwork for developing user-friendly applications that promote sustainable behaviors in residential energy usage. This research contributes valuable guidelines for developers and stakeholders to create more efficient and user-friendly applications, thus promoting environmental action and sustainable practices in residential settings.
Automated Lung Ultrasound Pulmonary Disease Quantification Using an Unsupervised Machine Learning Technique for COVID-19
Authors: Sagreiya Hersh , Jacobs Michael A. , Akhbardeh Alireza
Published: August 2023

COVID-19 is an ongoing global health pandemic. Although COVID-19 can be diagnosed with various tests such as PCR, these tests do not establish pulmonary disease burden. Whereas point-of-care lung ultrasound (POCUS) can directly assess the severity of characteristic pulmonary findings of COVID-19, the advantage of using US is that it is inexpensive, portable, and widely available for use in many clinical settings. For automated assessment of pulmonary findings, we have developed an unsupervised learning technique termed the calculated lung ultrasound (CLU) index. The CLU can quantify various types of lung findings, such as A or B lines, consolidations, and pleural effusions, and it uses these findings to calculate a CLU index score, which is a quantitative measure of pulmonary disease burden. This is accomplished using an unsupervised, patient-specific approach that does not require training on a large dataset. The CLU was tested on 52 lung ultrasound examinations from several institutions. CLU demonstrated excellent concordance with radiologist findings in different pulmonary disease states. Given the global nature of COVID-19, the CLU would be useful for sonographers and physicians in resource-strapped areas with limited ultrasound training and diagnostic capacities for more accurate assessment of pulmonary status.
Recognition of Rubber Tree Powdery Mildew Based on UAV Remote Sensing with Different Spatial Resolutions
Authors: Zeng Tiwei , Fang Jihua , Yin Chenghai , Li Yuan , Fu Wei , Zhang Huiming , Wang Juan , Zhang Xirui
Published: August 2023

Rubber tree is one of the essential tropical economic crops, and rubber tree powdery mildew (PM) is the most damaging disease to the growth of rubber trees. Accurate and timely detection of PM is the key to preventing the large-scale spread of PM. Recently, unmanned aerial vehicle (UAV) remote sensing technology has been widely used in the field of agroforestry. The objective of this study was to establish a method for identifying rubber trees infected or uninfected by PM using UAV-based multispectral images. We resampled the original multispectral image with 3.4 cm spatial resolution to multispectral images with different spatial resolutions (7 cm, 14 cm, and 30 cm) using the nearest neighbor method, extracted 22 vegetation index features and 40 texture features to construct the initial feature space, and then used the SPA, ReliefF, and Boruta–SHAP algorithms to optimize the feature space. Finally, a rubber tree PM monitoring model was constructed based on the optimized features as input combined with KNN, RF, and SVM algorithms. The results show that the simulation of images with different spatial resolutions indicates that, with resolutions higher than 7 cm, a promising classification result (>90%) is achieved in all feature sets and three optimized feature subsets, in which the 3.4 cm resolution is the highest and better than 7 cm, 14 cm, and 30 cm. Meanwhile, the best classification accuracy was achieved by combining the Boruta–SHAP optimized feature subset and SVM model, which were 98.16%, 96.32%, 95.71%, and 88.34% at 3.4 cm, 7 cm, 14 cm, and 30 cm resolutions, respectively. Compared with SPA–SVM and ReliefF–SVM, the classification accuracy was improved by 6.14%, 5.52%, 12.89%, and 9.2% and 1.84%, 0.61%, 1.23%, and 6.13%, respectively. This study’s results will guide rubber tree plantation management and PM monitoring.
The Teacher Image Interpretation in Student Teachers: A Linguistic Anthropology Approach
Authors: Zakirova Oksana , Bakhmutsky Andrei
Published: August 2023

The study aims to determine whether the images of a teacher in the normative document and the minds of student teachers coincide, as well as to identify the differences. A total of 236 students of the Faculty of Philology and History participated in the associative experiment. The words “ability”, “readiness”, and “possession” are the most common words used in the normative documents of the Russian Federation in the context of higher education. The concept of “teacher” is the main stimulus word in the associative experiment. The results of the associative experiment revealed a group of the most frequent associative words. In addition, based on the quantitative indicators of the associative experiment, nine thematic groups of associative words were formed. A comparative analysis of the indicators of an associative experiment involving students from different countries can make it possible to compare the features of national education systems and show their effect on the formation of a teacher image in the minds of student teachers.
A Method for Generating Geometric Image Sequences for Non-Isomorphic 3D-Mesh Sequence Compression
Authors: Gao Yuan , Wang Zhiqiang , Wen Jin
Published: August 2023

As virtual reality and 3D-modeling technology continue to advance, the amount of digital geometric media data is growing at an explosive rate. For example, 3D meshes, an important type of digital geometric media, can precisely record geometric information on a model’s surface. However, as the complexity and precision of 3D meshes increase, it becomes more challenging to store and transmit them. The traditional method of compressing non-isomorphic 3D-mesh sequences through frame-by-frame compression is inefficient and destroys the inter-frame correlations of the sequences. To tackle these issues, this study investigates the generation of time-dependent geometric image sequences for compressing non-isomorphic 3D-mesh sequences. Two methods are proposed for generating such sequences: one through image registration and the other through parametrization-geometry cooperative registration. Based on the experimental compression results of the video-coding algorithms, it was observed that the proposed geometric image-sequence-generation method offers superior objective and subjective qualities, as compared to the traditional method.
PreCaCycleGAN: Perceptual Capsule Cyclic Generative Adversarial Network for Industrial Defective Sample Augmentation
Authors: Yang Jiaxing , Wang Ke , Luan Fengkai , Yin Yong , Zhang Hu
Published: August 2023

Machine vision is essential for intelligent industrial manufacturing driven by Industry 4.0, especially for surface defect detection of industrial products. However, this domain is facing sparse and imbalanced defect data and poor model generalization, affecting industrial efficiency and quality. We propose a perceptual capsule cycle generative adversarial network (PreCaCycleGAN) for industrial defect sample augmentation, generating realistic and diverse defect samples from defect-free real samples. PreCaCycleGAN enhances CycleGAN with a U-Net and DenseNet-based generator to improve defect feature propagation and reuse and adds a perceptual loss function and a capsule network to improve authenticity and semantic information of generated features, enabling richer and more realistic global and detailed features of defect samples. We experiment on ten datasets, splitting each dataset into training and testing sets to evaluate model generalization across datasets. We train three defect detection models (YOLOv5, SSD, and Faster-RCNN) with original data and augmented data from PreCaCycleGAN and other state-of-the-art methods, such as CycleGAN-TSS and Tree-CycleGAN, and validate them on different datasets. Results show that PreCaCycleGAN improves detection accuracy and rate and reduces the false detection rate of detection models compared to other methods on different datasets, demonstrating its robustness and generalization under various defect conditions.
A Numerical Study on Labyrinth Screw Pump (LSP) Performance under Viscous Fluid Flow
Authors: Ke Wenqi , Zeng Hao , Wang Zhuoyu , Yu Hong , Liu Yaxin , Zheng Danzhu , Zhu Jianjun , Zhu Haiwen
Published: August 2023

In this study, fluid viscosity effects on LSP performance in terms of boosting pressure were numerically investigated. A water–glycerin mixture with different concentrations corresponding to varying apparent viscosities was flowed through an in-house manufactured LSP under various flow conditions, e.g., changing flow rates, rotational speeds, and fluid viscosities. The pressure increment between the intake and discharge of the LSP was recorded using the differential pressure transducer. The same pump geometries, fluid properties and flow conditions were incorporated into the numerical configurations, where three-dimensional (3D), steady-state, Reynolds-averaged Navier–Stokes (RANS) equations with a standard SST (shear stress transport) turbulence model were solved by a commercial CFD code. With the high-quality poly-hexcore grids, the simulated pressure increment was compared with the corresponding experimental measurement. The internal flow structures and characteristics within the cavities contained by the LSP impeller and diffuser were also analyzed. The good agreement between the numerical results against the experimental data verified the methodology adopted in this study.
Modeling and Control of a Hybrid-Fed Triple-Active Bridge Converter
Authors: Tarraf Rebecca , Frey David , Leirens Sylvain , Carcouet Sebastien , Maynard Xavier , Lembeye Yves
Published: August 2023

In general, the structures of Multi-Active Bridge (MAB) converters that can be found in the literature are usually based on voltage converters. However, in some cases, it could be interesting to have a current-fed input due to load characteristics or operation constraints. This leads to a hybrid MAB structure mixing both current-fed and voltage-fed bridges. In this paper, a new hybrid-fed, fully coupled Triple-Active Bridge (TAB) converter topology with two voltage-fed ports and one current-fed port is studied, modelled and controlled. In the first place, a generalized average model (GAM) is developed for this system. After that, a reduced-order model is elaborated in order to simplify the behavioral study and control of this coupled system. A control strategy was also proposed in this paper, based on the developed mathematical model. Simulation results using Matlab/Simulink are presented to validate this study.
Effects of Distance and Neighbor Size on Abies hickelii: The Asymmetric Competition Is Aggravated in an Endangered Species
Authors: Antúnez Pablo , Hernández-Cruz Iván , Ibrahim-Abdulsalam Fatima , Clark-Tapia Ricardo , Ruiz-Aquino Faustino , Valenzuela-Encinas César
Published: August 2023

One of the hypotheses in the field of forest ecology suggests that competition among individual plants is asymmetric or unidirectional. This notion holds particular significance for endangered species, necessitating a comprehensive assessment of the impact of their closest competitors to understand their spatial distribution and local-scale interactions. The mean response of Abies hickelii’s diameter at breast height and total height was determined for each unit that increased the distance from its neighbor and each unit that changed the size of its neighbor. The results obtained through the analysis of covariance suggest that, for each meter that the distance from the neighbor increases, there is a mean gain of 1.13 cm in the diameter of the individuals studied. The height results showed higher sensitivity to changes in the neighbor distance than the diameter. It was observed that the population is exposed to asymmetric competition, suggesting that larger individuals take available resources disproportionately, thereby inhibiting the growth of smaller individuals. The intraspecific competition surpassed the interspecific competition. A suggestion was made to use selective thinning during the early-growth stages of the species. The habitat of the species studied is under strong pressure from land use change due to clandestine logging, the opening of roads, and other anthropogenic effects. Thus, these results represent an important step in the implementation of action strategies to preserve the species in the wild.
Thyme (Thymus quinquecostatus Celak) Polyphenol-Rich Extract (TPE) Alleviates HFD-Induced Liver Injury in Mice by Inactivating the TLR4/NF-κB Signaling Pathway through the Gut–Liver Axis
Authors: Sheng Xialu , Wang Lixia , Zhan Ping , He Wanying , Tian Honglei , Liu Jianshu
Published: August 2023

Non-alcoholic fatty liver disease (NAFLD) represents a significant and urgent global health concern. Thyme (Thymus quinquecostatus Celak) is a plant commonly used in cuisine and traditional medicine in Asian countries and possesses potential liver-protective properties. This study aimed to assess the hepatoprotective effects of thyme polyphenol-rich extract (TPE) on high-fat diet (HFD)-induced NAFLD and further explore possible mechanisms based on the gut–liver axis. HFD-induced liver injury in C57 mice is markedly ameliorated by TPE supplementation in a dose-dependent manner. TPE also regulates the expression of liver lipid metabolic genes (i.e., Hmgcr, Srebp-1, Fasn, and Cyp7a1), enhancing the production of SCFAs and regulating serum metabolites by modulating gut microbial dysbiosis. Furthermore, TPE enhances the intestinal barrier function and alleviates intestinal inflammation by upregulating tight junction protein expression (i.e., ZO-1 and occluding) and inactivating the intestinal TLR4/NF-κB pathway in HFD-fed mice. Consequently, gut-derived LPS translocation to the circulation was blocked, the liver TLR4/NF-κB signaling pathway was repressed, and subsequent pro-inflammatory cytokine production was restrained. Conclusively, TPE might exert anti-NAFLD effects through the gut–liver axis and has the potential to be used as a dietary supplement for the management of NAFLD.
Temperature, Salinity and Garlic Additive Shape the Microbial Community during Traditional Beetroot Fermentation Process
Authors: Staninska-Pięta Justyna , Czarny Jakub , Wolko Łukasz , Cyplik Paweł , Drożdżyńska Agnieszka , Przybylak Martyna , Ratajczak Katarzyna , Piotrowska-Cyplik Agnieszka
Published: August 2023

Plant-based traditional fermented products are attracting a lot of interest in global markets. An example of them is beetroot leaven, which is valued for its high bioactive compound content. The variety of production recipes and the spontaneous nature of red beet fermentation favor its high diversity. This study aimed to analyze the impact of external factors—temperature, brine salinity, and garlic dose—on the beetroot fermentation and bacterial metapopulation responsible for this process. The research results confirmed the significant influence of the selected and analyzed factors in shaping the leaven physicochemical profile including organic acid profile and betalain content. Analysis of bacterial populations proved the crucial importance of the first 48 h of the fermentation process in establishing a stable metapopulation structure and confirmed that this is a targeted process driven by the effect of the analyzed factors. Lactobacillaceae, Enterobacteriaceae, and Leuconostocaceae were observed to be the core microbiome families of the fermented red beet. Regardless of the impact of the tested factors, the leaven maintained the status of a promising source of probiotic bacteria. The results of this research may be helpful in the development of the regional food sector and in improving the quality and safety of traditionally fermented products such as beetroot leaven.
A Rare Case of Precocious Puberty in a Child with a Novel GATA-4 Gene Mutation: Implications for Disorders of Sex Development (DSD) and Review of the Literature
Authors: Aversa Tommaso , Luppino Giovanni , Corica Domenico , Pepe Giorgia , Valenzise Mariella , Coco Roberto , Li Pomi Alessandra , Wasniewska Malgorzata
Published: August 2023

Background: Disorders/Differences of sex development (DSD) are often due to disruptions of the genetic programs that regulate gonad development. The GATA-4 gene, located on chromosome 8p23.1, encodes GATA-binding protein 4 (GATA-4), a transcription factor that is essential for cardiac and gonadal development and sexual differentiation. Case Description: A child with a history of micropenis and cryptorchidism. At 8 years of age, he came under our observation for an increase in sexual pubic hair (pubarche). The laboratory parameters and the GnRH test suggested a central precocious puberty (CPP). Treatment with GnRH analogs was started, and we decided to perform genetic tests for DSD. The NGS genetic investigation showed a novel and heterozygous variant in the GATA-4 gene. Discussion: In the literature, 26 cases with 46,XY DSD due to the GATA4 gene were reported. Conclusion: The novel variant in the GATA-4 gene of our patient was not previously associated with DSD. This is the first case of a DSD due to a GATA-4 mutation that develops precocious puberty. Precocious puberty could be associated with DSD and considered a prelude to hypogonadism in some cases.
Differences of Disabling Symptoms between Previously Hospitalized or Non-Hospitalized Currently Working Long-COVID Survivors One Year after Infection: A Descriptive Study
Authors: López-López Laura , Calvache-Mateo Andrés , Ortiz-Rubio Araceli , Granados-Santiago María , Heredia-Ciuró Alejandro , Martín-Núñez Javier , Valenza Marie Carmen
Published: August 2023

This study aimed to describe the presence of disabling symptoms in currently working Long-COVID survivors by comparing the hospitalized and non-hospitalized one year after infection. Patients with Long-COVID syndrome (LCS) that have been infected by COVID-19 a year ago and were actually working were included. Participants that had been hospitalized due to COVID-19 were included in the LCS hospitalized group, and participants that had not been hospitalized were included in the LCS non-hospitalized group. The eligible patients were prompted to complete the latest self-report version of the COVID-19 Yorkshire Rehabilitation Screening Tool (C19-YRS). A total of 465 subjects were included in the study. Participants in the LCS hospitalized group were significantly older, had a significantly higher BMI, and had a significantly higher prevalence of women compared to the LCS non-hospitalized group. Additionally, participants in the LCS hospitalized group had obtained significantly worse results in symptom severity, functional disability, and global health perceived subscales of C19-YRS compared to the participants included in the LCS non-hospitalized group. We concluded that disabling symptoms are presented in patients with LCS at working age one year after infection and are higher in LCS hospitalized patients compared to LCS non-hospitalized patients.
Inappropriate Prescribing of Antibiotics to Pediatric Patients Receiving Medicaid: Comparison of High-Volume and Non-High-Volume Antibiotic Prescribers—Kentucky, 2019
Authors: Wattles Bethany A. , Smith Michael J. , Feygin Yana , Jawad Kahir , Flinchum Andrea , Corley Brittany , Spicer Kevin B.
Published: August 2023

Inappropriate antibiotic prescribing to pediatric Medicaid patients was compared among high-volume and non-high-volume prescribers. High-volume prescribers had a higher percentage of inappropriate prescriptions than non-high-volume prescribers (17.2% versus 15.8%, p = 0.005). Targeting high-volume prescribers for stewardship efforts is a practical approach to reducing outpatient antibiotic prescribing that also captures inappropriate use.
Construct Validity of a Questionnaire on Eating and Physical Activity Habits for Adolescents in Mexico City
Authors: Martínez Coronado Araceli , Lazarevich Irina , Gutiérrez Tolentino Rey , Mejía Arias Miguel Ángel , Leija Alva Gerardo , Radilla Vázquez Claudia Cecilia
Published: August 2023

The assessment of eating and physical activity habits is an important step in promoting healthy behaviors among the adolescent population and is key in the prevention and management of chronic non-communicable diseases, such as obesity, diabetes, and cardiovascular disease. For this purpose, reliable and valid measuring instruments are essential. In this context, the aim of this article is to present the validation of a self-report questionnaire on eating and physical activity habits among adolescents in Mexico City. In order to validate the questionnaire, a cross-sectional study was conducted with a sample of 2710 adolescents between 11 and 12 years of age, the piloting of the questionnaire was carried out in September 2022 with a focus group, and the programming of the anthropometric measurements was established with the Federal Educational Authority of CDMX, as well as the application of the questionnaire to 33 schools, with these activities being scheduled from 7 November 2022 to 3 February 2023 and having an application duration of 15–25 min for each of the groups to which it was applied; the questionnaire that was applied consists of 31 questions that refer to the frequencies, quantity, or performance of behaviors related to the frequency and type of food, type of physical activity and behaviors related to the act of eating referring to the place where it is carried out (home or away from home) and with whom it is carried out (alone or in company), and about the individual’s lifestyle. Subsequently, the reliability of the instrument was evaluated using Cronbach’s alpha coefficient, and an exploratory factor analysis was conducted to determine the structure of the questionnaire. The results obtained showed that the questionnaire was adequately reliable (α = 0.778) with an eight-factor structure: four questions on mealtime frequencies, four questions on physical activity and lifestyles, six questions on the consumption of high-calorie foods, four questions on company and food consumption, four questions on the consumption of vegetables and fruits, four questions on the place of food consumption, two questions on the consumption of alcoholic beverages, and three questions on the consumption of sugary drinks, plain water, and milk. In conclusion, the self-report questionnaire on eating and physical activity habits among adolescents in Mexico City is reliable, has adequate internal consistency, and can therefore be used as a useful tool for the evaluation of eating and physical activity habits in this population.
The Distinct Innate Immune Response of Warm Ischemic Injured Livers during Continuous Normothermic Machine Perfusion
Authors: Blondeel Joris , Gilbo Nicholas , Heedfeld Veerle , Wylin Tine , Libbrecht Louis , Jochmans Ina , Pirenne Jacques , Korf Hannelie , Monbaliu Diethard
Published: August 2023

Although normothermic machine perfusion (NMP) provides superior preservation of liver grafts compared to static cold storage and allows for viability testing of high-risk grafts, its effect on the liver immune compartment remains unclear. We investigated the innate immune response during 6 h of continuous NMP (cNMP) of livers that were directly procured (DP, n = 5) or procured after 60 min warm ischemia (WI, n = 5), followed by 12 h of whole blood (WB) reperfusion. WI livers showed elevated transaminase levels during cNMP but not after WB reperfusion. Perfusate concentrations of TNF-α were lower in WI livers during cNMP and WB reperfusion, whereas IL-8 concentrations did not differ significantly. TGF-β concentrations were higher in WI livers during NMP but not after WB reperfusion, whereas IL-10 concentrations were similar. Endoplasmic stress and apoptotic signaling were increased in WI livers during cNMP but not after WB reperfusion. Additionally, neutrophil mobilization increased to a significantly lesser extent in WI livers at the end of NMP. In conclusion, WI livers exhibit a distinct innate immune response during cNMP compared to DP livers. The cytokine profile shifted towards an anti-inflammatory phenotype during cNMP and WB reperfusion, and pro-apoptotic signaling was stronger during cNMP. During WB reperfusion, livers exhibited a blunted cytokine release, regardless of ischemic damage, supporting the potential reconditioning effect of cNMP.
Antioxidants and Obesity
Authors: Bonomini Francesca
Published: August 2023

Obesity and its global prevalence are increasingly becoming serious worldwide risks [...]
Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
Authors: Kawai-Harada Yuki , El Itawi Hanine , Komuro Hiroaki , Harada Masako
Published: August 2023

Extracellular vesicles (EVs), detectable in all bodily fluids, mediate intercellular communication by transporting molecules between cells. The capacity of EVs to transport molecules between distant organs has drawn interest for clinical applications in diagnostics and therapeutics. Although EVs hold potential for nucleic-acid-based and other molecular therapeutics, the lack of standardized technologies, including isolation, characterization, and storage, leaves many challenges for clinical applications, potentially resulting in misinterpretation of crucial findings. Previously, several groups demonstrated the problems of commonly used storage methods that distort EV integrity. This work aims to evaluate the process to optimize the storage conditions of EVs and then characterize them according to the experimental conditions and the models used previously. Our study reports a highly efficient EV storage condition, focusing on EV capacity to protect their molecular cargo from biological, chemical, and mechanical damage. Compared with commonly used EV storage conditions, our EV storage buffer leads to less size and particle number variation at both 4 °C and −80 °C, enhancing the ability to protect EVs while maintaining targeting functionality.
Extracellular Cysteine Proteases of Key Intestinal Protozoan Pathogens—Factors Linked to Virulence and Pathogenicity
Authors: Argüello-García Raúl , Carrero Julio César , Ortega-Pierres M. Guadalupe
Published: August 2023

Intestinal diseases caused by protistan parasites of the genera Giardia (giardiasis), Entamoeba (amoebiasis), Cryptosporidium (cryptosporidiosis) and Blastocystis (blastocystosis) represent a major burden in human and animal populations worldwide due to the severity of diarrhea and/or inflammation in susceptible hosts. These pathogens interact with epithelial cells, promoting increased paracellular permeability and enterocyte cell death (mainly apoptosis), which precede physiological and immunological disorders. Some cell-surface-anchored and molecules secreted from these parasites function as virulence markers, of which peptide hydrolases, particularly cysteine proteases (CPs), are abundant and have versatile lytic activities. Upon secretion, CPs can affect host tissues and immune responses beyond the site of parasite colonization, thereby increasing the pathogens’ virulence. The four intestinal protists considered here are known to secrete predominantly clan A (C1- and C2-type) CPs, some of which have been characterized. CPs of Giardia duodenalis (e.g., Giardipain-1) and Entamoeba histolytica (EhCPs 1-6 and EhCP112) degrade mucin and villin, cause damage to intercellular junction proteins, induce apoptosis in epithelial cells and degrade immunoglobulins, cytokines and defensins. In Cryptosporidium, five Cryptopains are encoded in its genome, but only Cryptopains 4 and 5 are likely secreted. In Blastocystis sp., a legumain-activated CP, called Blastopain-1, and legumain itself have been detected in the extracellular medium, and the former has similar adverse effects on epithelial integrity and enterocyte survival. Due to their different functions, these enzymes could represent novel drug targets. Indeed, some promising results with CP inhibitors, such as vinyl sulfones (K11777 and WRR605), the garlic derivative, allicin, and purified amoebic CPs have been obtained in experimental models, suggesting that these enzymes might be useful drug targets.