bluuebunny/crossref_metadata_2025 huggingface dataset:
Gastrostomies: experience and complications with three modalities in a tertiary centre over a 26-year period
Authors: Piñar-Gutiérrez Ana , Serrano-Aguayo Pilar , Gutiérrez Rocío Vázquez , Rey Silvia García , González-Navarro Irene , Tatay-Domínguez Dolores , Garrancho-Domínguez Pilar , Remón-Ruiz Pablo J. , Martínez-Ortega Antonio J. , Mejías Verónica Nacarino , Iglesias-López Álvaro , Socas María , Morales-Conde Salvador , García-Fernández Francisco José , Bozada-García Juan Manuel , Pereira-Cunill José Luis , García-Luna Pedro Pablo
Published: October 2023

Objectives

To describe the complications associated with the different gastrostomy techniques [endoscopic (PEG), radiologic (PRG), and surgical (SG)] performed in the last 26 years in a terciary hospital.

Methods

Retrospective observational study. Patients who underwent gastrostomy at the Virgen del Rocío University Hospital between 1995 and 2021 were included. For PEG, the PULL technique was performed until 2018 and subsequently the PUSH technique predominantly. For PRG, a pigtail catheter was used until 2003, a balloon catheter between 2003 and 2009, and a balloon catheter with gastropexy between 2015 and 2021. For SG, the conventional technique (CSG) was performed until 2009 and since then the laparoscopic assisted percutaneous gastrostomy (PLAG) technique. Descriptive analysis was performed obtaining the median and quartiles of the quantitative variables [P50 (P25-P75)] and the frequency for the qualitative variables [n (%)].The comparison of complications between patients who underwent different techniques was performed with Fisher’s test.

Results

n = 1,070 (PEG = 608, PRG = 344, SG = 118). The three most frequent indications were head and neck tumors, neurological diseases and gastroesophageal tumors. The percentage of patients who had any complication was 48.9% (PEG-PULL), 23.7% (PEG-PUSH), 38.5% (pigtail PRG), 39.2% (balloon PRG), 29.7% (balloon with gastropexy PRG), 87.3% (CSG), and 41.26% (PLAG). 2 (0.18%) patients died from gastrostomy-related complications. 18(1.68%) presented with peritonitis and 5 (0.4%) presented with gastrocolic fistula. The rest of the complications were minor.

Conclusion

Gastrostomy in any of its modalities is currently a safe procedure with a low rate of complications, most of which are minor.
A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea
Authors: Lu Ping , Wang Ke , Wang Jiaqi , Xia Chunbo , Yang Shu , Ma Liang , Shi Haojie
Published: October 2023

Reactive oxygen species (ROS) are important for plant defense against fungal attack. As a necrotrophic fungus, Botrytis cinerea can exploit ROS that originated from both sides of the host and pathogen during interaction to facilitate its infestation. Meanwhile, B. cinerea needs to exert an efficient oxidative stress responsive system to balance the intracellular redox state when encountering deleterious ROS levels. However, the machinery applied by B. cinerea to cope with ROS remains obscure. Herein, we investigated the role of the transcription factor BcMsn2 in regulating B. cinerea redox homeostasis. Disruption of the BcMsn2 gene severely impaired vegetative growth, sclerotium formation, conidial yield, and fungal virulence. The intracellular oxidative homeostasis of the ∆bcmsn2 mutant was disrupted, leading to significantly elevated levels of ROS and reduced activities of enzymes closely associated with oxygen stress, such as catalase (CAT) and superoxide dismutase (SOD). RNA-Seq and qRT-PCR analyses showed remarkable downregulation of the expression of several genes encoding ROS scavenging factors involved in maintaining the redox homeostasis in ∆bcmsn2, suggesting that BcMsn2 functions as a transcriptional regulator of these genes. Our findings indicated that BcMsn2 plays an indispensable role in maintaining the equilibrium of the redox state in B. cinerea, and intracellular ROS serve as signaling molecules that regulate the growth, asexual reproduction, and virulence of this pathogen.
A glucose-mediated antibiotic resistance metabolic flux from glycolysis, the pyruvate cycle, and glutamate metabolism to purine metabolism
Authors: Xiang Jiao , Wang Shi-wen , Tao Yuan , Ye Jing-zhou , Liang Ying , Peng Xuan-xian , Yang Li-fen , Li Hui
Published: October 2023

Introduction

Bacterial metabolic environment influences antibiotic killing efficacy. Thus, a full understanding for the metabolic resistance mechanisms is especially important to combat antibiotic-resistant bacteria.

Methods

Isobaric tags for relative and absolute quantification-based proteomics approach was employed to compare proteomes between ceftazidime-resistant and -sensitive Edwarsiella tarda LTB4 (LTB4-RCAZ and LTB4-S, respectively).

Results

This analysis suggested the possibility that the ceftazidime resistance mediated by depressed glucose is implemented through an inefficient metabolic flux from glycolysis, the pyruvate cycle, glutamate metabolism to purine metabolism. The inefficient flux was demonstrated by the reduced expression of genes and the decreased activity of enzymes in the four metabolic pathways. However, supplement upstream glucose and downstream guanosine separately restored ceftazidime killing, which not only supports the conclusion that the inefficient metabolic flux is responsible for the resistance, but also provides an effective approach to reverse the resistance. In addition, the present study showed that ceftazidime is bound to pts promoter in E. tarda.

Discussion

Our study highlights the way in fully understanding metabolic resistance mechanisms and establishing metabolites-based metabolic reprogramming to combat antibiotic resistance.
A gap between reasons for skilled use of BCI speech devices and reasons for utterances, with implications for speech ownership
Authors: Rainey Stephen
Published: October 2023

The skilled use of a speech BCI device will draw upon practical experience gained through the use of that very device. The reasons a user may have for using a device in a particular way, reflecting that skill gained via familiarity with the device, may differ significantly from the reasons that a speaker might have for their utterances. The potential divergence between reasons constituting skilled use and BCI-mediated speech output may serve to make clear an instrumental relationship between speaker and BCI speech device. This will affect the way in which the device and the speech it produces for the user can be thought of as being “reasons responsive”, hence the way in which the user can be said to be in control of their device. Ultimately, this divergence will come down to how ownership of produced speech can be considered. The upshot will be that skillful use of a synthetic speech device might include practices that diverge from standard speech in significant ways. This might further indicate that synthetic speech devices ought to be considered as different from, not continuous with, standard speech.
A nutritional supplement taken during preconception and pregnancy influences human milk macronutrients in women with overweight/obesity and gestational diabetes mellitus
Authors: Han Soo Min , Derraik José G. B. , Vickers Mark H. , Devaraj Surabhi , Huang Fang , Pang Wei Wei , Godfrey Keith M. , Chan Shiao-Yng , Thakkar Sagar K. , Cutfield Wayne S. ,   NiPPeR Study Group
Published: October 2023

Rational

Maternal overweight/obesity and gestational diabetes mellitus (GDM) are associated with an increased risk of their offspring developing overweight/obesity or type 2 diabetes later in life. However, the impacts of maternal overweight/obesity and dysglycemia on human milk (HM) macronutrient composition are not well understood.

Objective

Through a double-blind randomised controlled trial, we investigated the effects of maternal supplementation from preconception throughout pregnancy until birth on HM macronutrient concentrations, in association with maternal and infant factors including maternal pre-pregnancy body mass index (BMI) and GDM status. In addition, we aimed to characterise longitudinal changes in HM macronutrients.

Methods

The control supplement contained calcium, iodine, iron, β-carotene, and folic acid. The intervention supplement additionally contained zinc, vitamins B2, B6, B12, and D3, probiotics, and myo-inositol. HM samples were collected across seven time points from 1 week to 12 months from Singapore and/or New Zealand. HM macronutrient concentrations were measured using a MIRIS Human Milk Analyser. Potential differences in HM macronutrient concentrations were assessed using linear mixed models with a repeated measures design.

Results

Overall, HM macronutrient concentrations were similar between control and intervention groups. Among the control group, overweight/obesity and GDM were associated with higher HM fat and energy concentrations over the first 3 months. Such associations were not observed among the intervention group. Of note, mothers with GDM in the intervention group had lower HM fat by 10% (p = 0.049) and energy by 6% (p = 0.029) than mothers with GDM in the control group. Longitudinal changes in HM macronutrient concentrations over 12 months of lactation in New Zealand showed that HM fat and energy decreased in the first 6 months then increased until 12 months. HM lactose gradually decreased from 1 week to 12 months while crude protein decreased from 1 week to 6 months then remained relatively constant until 12 months of lactation.

Conclusion

Maternal overweight/obesity or GDM were associated with increased HM fat and energy levels. We speculate the intervention taken during preconception and pregnancy altered the impact of maternal BMI or GDM status on HM macronutrient composition. Further studies are required to identify the mechanisms underlying altered HM macronutrient concentration in the intervention group and to determine any long-term effects on offspring health.

Clinical trial registration

ClinicalTrials.gov, NCT02509988, Universal Trial Number U1111-1171-8056. Registered on 16 July 2015. This is an academic-led study by the EpiGen Global Research Consortium.
Preoperative hematocrit levels and postoperative mortality in patients undergoing craniotomy for brain tumors
Authors: Xiao Yangchun , Cheng Xin , Jia Lu , Tian Yixin , He Jialing , He Miao , Chen Lvlin , Hao Pengfei , Li Tiangui , Chong Weelic , Hai Yang , You Chao , Peng Liyuan , Fang Fang , Zhang Yu
Published: October 2023

Background

Abnormal hematocrit values, including anemia and polycythemia, are common in patients undergoing craniotomy, but the extent to which preoperative anemia or polycythemia independently increases the risk of mortality is unclear. This retrospective cohort study aimed to examine the association between preoperative anemia and polycythemia and postoperative mortality in patients who underwent craniotomy for brain tumor resection.

Methods

We retrospectively analyzed data from 12,170 patients diagnosed with a brain tumor who underwent cranial surgery at West China Hospital between January 2011 and March 2021. The preoperative hematocrit value was defined as the last hematocrit value within 7 days before the operation, and patients were grouped according to the severity of their anemia or polycythemia. We assessed the primary outcome of 30-day postoperative mortality using logistic regression analysis adjusted for potential confounding factors.

Results

Multivariable logistic regression analysis reported that the 30-day mortality risk was raised with increasing severity of both anemia and polycythemia. Odds ratios for mild, moderate, and severe anemia were 1.12 (95% CI: 0.79–1.60), 1.66 (95% CI: 1.06–2.58), and 2.24 (95% CI: 0.99–5.06), respectively. Odds ratios for mild, moderate, and severe polycythemia were 1.40 (95% CI: 0.95–2.07), 2.81 (95% CI: 1.32–5.99), and 14.32 (95% CI: 3.84–53.44), respectively.

Conclusions

This study demonstrated that moderate to severe anemia and polycythemia are independently associated with increased postoperative mortality in patients undergoing craniotomy for brain tumor resection. These findings underscore the importance of identifying and managing abnormal hematocrit values before craniotomy surgery.
Long-read sequencing of oropharyngeal squamous cell carcinoma tumors reveal diverse patterns of high-risk Human Papillomavirus integration
Authors: Gauthier Marc-Andre , Kadam Adway , Leveque Gary , Golabi Nahid , Zeitouni Anthony , Richardson Keith , Mascarella Marco , Sadeghi Nader , Loganathan Sampath Kumar
Published: October 2023

Introduction

In North America and in most European countries, Human Papillomavirus (HPV) is responsible for over 70% of oropharyngeal squamous cell carcinomas. The burden of OPSCC, in high-income countries, has been steadily increasing over the past 20 years. As a result, in the USA and in the UK, the burden of HPV-related oropharyngeal squamous cell carcinoma in men has now surpassed that of cervical cancer in women. However, the oncogenic impact of high-risk HPV integration in oropharyngeal squamous cell carcinomas hasn’t been extensively studied. The present study aimed to explore the patterns of HPV integration in oropharyngeal squamous cell carcinomas and to assess the feasibility and reliability of long-read sequencing technology in detecting viral integration events in oropharyngeal head and neck cancers.

Methods

A cohort of eight HPV-positive OPSCC pre-treatment patient tumors (four males and four females), were selected. All patients received a p16INK4A positive OPSCC diagnosis and were treated at the McGill University Health Centre, a quaternary center in Montreal. A minimum of 20mg of tumor tissue was used for DNA extraction. Extracted DNA was subjected to Nanopore long-read sequencing to detect and analyze for the presence of high-risk HPV sequences. PCR and Sanger sequencing experiments were performed to confirm Nanopore long-read sequencing readings.

Results

Nanopore long-read sequencing showed that seven out of eight patient samples displayed either integrated or episomal high-risk HPV sequences. Out of these seven samples, four displayed verifiable integration events upon bioinformatic analysis. Integration confirmation experiments were designed for all four samples using PCR-based methods. Sanger sequencing was also performed. Four distinct HPV integration patterns were identified: concatemer chromosomal integration in a single chromosome, bi-chromosomal concatemer integration, single chromosome complete integration and bi-chromosomal complete integration. HPV concatemer integration also proved more common than full HPV integration events.

Conclusion and relevance

Long-read sequencing technologies can be effectively used to assess HPV integration patterns in OPSCC tumors. Clinically, more research should be conducted on the prognostication value of high-risk HPV integration in OPSCC tumors using long-read sequencing technology.
The top 100 most cited articles in helical tomotherapy: a scoping review
Authors: Zhang Yadong , Rong Liyi , Wang Zhiqiang , Zhao Hongfu
Published: October 2023

Objective

The purpose of this scoping review was to explore the top 100 most cited articles in helical tomotherapy (HT) through bibliometric analysis and visualization tools, help researchers comprehensively understand the research hotspots of HT, and provide clear and intuitive network visualization.

Methods

The Web of Science Core Collection and the search strategy of “Title (TI)=(tomotherapy)” were used to search for articles related to HT as of 27 May 2023. The top 100 most cited articles were obtained by sorting “citations: highest first”. From these top 100 most cited articles, the following information was extracted: journals, years and months, countries, authors, types of tumor treated, and topics. The VOSviewer software was introduced for visualizing all the articles related to HT.

Results

The top 100 most cited articles in HT were published between 1999 and 2019. The citation counts of these articles ranges from 326 to 45, with a total of 8,422 citations at the time of searching. The index of citations per year (CPY) ranges from 22.32 to 2.45. These articles originated from 17 countries, with most publications from the United States (n=50), followed by Canada (n=12), Italy (n=10), Germany (n=7) and Belgium (n=5). The International Journal of Radiation Oncology, Biology, Physics published the highest number of articles (n=31), followed by Radiotherapy and Oncology (n=20), Medical Physics (n=13) and Strahlentherapie und Onkologie (n=12). In terms of specific tumor types, head and neck cancer (n=15) is the most common disease, followed by cancers with complex target structures (n=14), breast cancer (n=12), prostate cancer (n=10) and lung cancer (n=8). The most common research topics also include dosimetric comparison (n = 44), quality assurance (n = 12) and Megavoltage CT (n = 8).

Conclusion

This scoping review provides a comprehensive list of the 100 most cited articles in HT. This analysis offers valuable insights into the current research directions of HT that can be utilized by researchers, clinicians, and policy-makers.
The role of matrix stiffness in breast cancer progression: a review
Authors: Xu Ruoxi , Yin Peng , Wei Jifu , Ding Qiang
Published: October 2023

The significance of matrix stiffness in cancer development has been investigated in recent years. The gradual elastic force the extracellular matrix imparts to cells, known as matrix stiffness, is one of the most important types of mechanical stimulation. Increased matrix stiffness alters the biological activity of cells, which promotes the growth of numerous malignancies, including breast cancer. Comprehensive studies have demonstrated that increasing matrix stiffness activates molecular signaling pathways that are closely linked to breast cancer progression. There are many articles exploring the relationship between mechanism hardness and breast cancer, so we wanted to provide a systematic summary of recent research advances. In this review, we briefly introduce the mechanism of matrix stiffness in breast cancer, elaborate on the effect of extracellular matrix stiffness on breast cancer biological behavior and signaling pathways, and finally, we will talk about breast cancer treatment that focuses on matrix stiffness.
Mycophenolate mofetil for autoimmune cytopenias in children: high rates of response in inborn errors of immunity
Authors: Berrueco Rubén , González-Forster Elisa , Deya-Martinez Angela , Solsona María , García-García Ana , Calzada-Hernández Joan , Yiyi Luo , Vlagea Alexandru , Ruiz-Llobet Anna , Alsina Laia
Published: October 2023

Second-line treatments of autoimmune cytopenias (AC) are not well-defined in children. Mycophenolate mofetil (MMF) is an immunosuppressant agent that has been demonstrated to be safe and effective in this setting. A retrospective observational study was conducted in 18 children with prolonged AC who received MMF, in order to describe clinical and biological markers of response. The overall response rate of MMF at 20–30 mg/kg per day was 73.3%. All patients with Evans syndrome (n = 9) achieved complete response. Among the patients with monolineage AC (n = 9), those with an underlying inborn errors of immunity (IEI), tended to respond better to MMF. No biological markers related to treatment response were found. Rather, lymphocyte subpopulations proved useful for patient selection as a marker suggestive of IEI along with immunoglobulin-level determination.
Methotrexate-conjugated zinc oxide nanoparticles exert a substantially improved cytotoxic effect on lung cancer cells by inducing apoptosis
Authors: Mishra Prakriti , Ali Ahmad Mohd Faizan , Al-Keridis Lamya Ahmed , Saeed Mohd , Alshammari Nawaf , Alabdallah Nadiyah M. , Tiwari Rohit Kumar , Ahmad Afza , Verma Mahima , Fatima Shireen , Ansari Irfan Ahmad
Published: October 2023

In the current study, we report the synthesis of methotrexate-conjugated zinc oxide nanoparticles (MTX-ZnONPs) and their high efficacy against lung cancer cells. Conjugation of MTX with ZnONPs was authenticated by UV-vis spectroscopy, dynamic light scattering (DLS), Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). This drug-nanoconjugate also showed high drug-loading efficiency. The therapeutic efficacy of MTX-ZnONPs was further tested in vitro against A549 cells, and the results of MTT and LDH release assays showed that MTX-ZnONPs, in addition to free MTX, were efficient in exerting cytotoxic effect on A549 cells; however, the effectiveness of MTX-ZnONPs was found to be considerably enhanced at very low doses compared to that of free MTX. Moreover, ZnONPs alone significantly inhibited the cell viability of A549 cells at a much higher concentration compared to MTX-ZnONPs and MTX. Furthermore, the cytomorphology of A549 cells was characterized by cellular shrinkage and detachment from the surface in all the treatment groups. Similarly, A549 cells, in all the treatment groups, showed fragmented and condensed nuclei, indicating the initiation of apoptosis. Mitochondrial membrane potential (ψm) in A549 cells showed a gradual loss in all the treatment groups. Results of the qualitative and quantitative analyses depicted increased reactive oxygen species (ROS) levels in A549 cells. The results of the caspase activity assay showed that MTX-ZnONPs andfree MTX caused significant activation of caspase-9, -8, and -3 in A549 cells; however, the effect of MTX-ZnONPs was more profound at very low doses compared to that of free MTX. Thus, our results showed high efficacy of MTX-ZnONPs, suggesting efficient intracellular delivery of the drug by ZnONPs as nanocarriers.
Potential short-term effects of earthquake on the plant–soil interface in alpine grassland of the Qinghai–Tibetan Plateau
Authors: Zuo Hui , Shen Hao , Dong Shikui , Wu Shengnan , He Fengcai , Zhang Ran , Wang Ziying , Shi Hang , Hao Xinghai , Tan Youquan , Ma Chunhui , Li Shengmei , Liu Yongqi , Zhang Feng , Xiao Jiannan
Published: October 2023

Earthquakes are environmental disturbances affecting ecosystem functioning, health, and biodiversity, but their potential impacts on plant–soil interface are still poorly understood. In this study, grassland habitats in areas near and away from the seismo-fault in Madou, a region typical of alpine conditions on the Qinghai–Tibetan Plateau, were randomly selected. The impacts of earthquake on soil properties and plant nutrient content in the short term were emphasized, and their potential relationships with community diversity and productivity were examined. According to the findings of the study, the Maduo earthquake led to a decrease in soil nutrient content in alpine grassland ecosystems, especially soil TC, TN, TP, TCa, AP, AK, NH4+-N, and SOC, and inhibited the absorption of N, Ca, and Mg nutrients by plants. In addition, the diversity and productivity of communities were affected by both direct and indirect earthquake pathways. The negative impacts of seismic fracture on soil structure had the most significant direct impact on plant community diversity. Earthquakes also indirectly reduced community productivity by reducing the soil N content and inhibiting the absorption of plant nutrients. Our findings suggested that earthquakes could potentially decrease the stability of the alpine grassland ecosystem on the QTP by affecting nutrient availability at the plant–soil interface.
Romantic love evolved by co-opting mother-infant bonding
Authors: Bode Adam
Published: October 2023

For 25 years, the predominant evolutionary theory of romantic love has been Fisher’s theory of independent emotion systems. That theory suggests that sex drive, romantic attraction (romantic love), and attachment are associated with distinct neurobiological and endocrinological systems which evolved independently of each other. Psychological and neurobiological evidence, however, suggest that a competing theory requires attention. A theory of co-opting mother-infant bonding sometime in the recent evolutionary history of humans may partially account for the evolution of romantic love. I present a case for this theory and a new approach to the science of romantic love drawing on human psychological, neurobiological, and (neuro)endocrinological studies as well as animal studies. The hope is that this theoretical review, along with other publications, will generate debate in the literature about the merits of the theory of co-opting mother-infant bonding and a new evolutionary approach to the science of romantic love.
Efficacy for writing self-regulation, attitude toward writing, and quality of second grade students’ writing
Authors: Skar Gustaf B. , Graham Steve , Huebner Alan R.
Published: October 2023

Motivational beliefs, such as writing self-efficacy and attitude toward writing, are believed to foster or hinder writing by influencing if one chooses to write, how much effort is committed to writing, and what cognitive resources writers apply. In the current study, we examined self-efficacy for writing self-regulation and attitude toward writing of 2,124 Grade 2 Norwegian students (1,069 girls; 1,055 boys). We investigated if there were differences in each of these beliefs between girls and boys and students who differed in their language status (Norwegian first language, Norwegian and another language both first language, or language other than Norwegian first language). We further tested if each of these writing motivational beliefs made statistically unique contributions to predicting the quality of students’ writing. In each of these analyses, we controlled for variance related to individual- and school factors. Girls were more positive about writing than boys, and they were confident about their abilities to self-regulate writing. Students with Norwegian and another language both as first language (“bilingual” students) had a more positive attitude toward writing than the other two language groups. Efficacy for writing self-regulation and attitude toward writing both made statistically significant unique contributions to predicting the quality of students writing, although these two writing beliefs collectively accounted for just 2% of the variance in writing quality scores once individual- and school-factors were controlled. Recommendations for future research and implications of the finding are discussed.
NIH-supported implementation science and nutrition research: a portfolio review of the past decade
Authors: Vorkoper Susan , Korn Ariella R. , Maruvada Padma , Nicastro Holly L. , Shi Scarlet
Published: October 2023

Objective

This portfolio analysis aims to describe the scope of NIH-funded extramural research grants at the intersection of nutrition research and implementation science (IS) from 2011 to 2022 and to offer insights into future research opportunities relevant to the Strategic Plan for NIH Nutrition Research 2020–2030.

Methods

A portfolio analysis of funded grants using NIH reporting systems was conducted to identify nutrition research and IS awarded between fiscal years 2011 and 2022. The authors screened the titles and abstracts for inclusion criteria: research and career development awards involved a nutrition and/or dietary intervention and measured a stated implementation outcome or used an IS theory, model, or framework.

Results

In total, 33 NIH-funded awards met the inclusion criteria. Almost half of the awards (48.5%) were investigator-initiated research projects compared to research career awards and cooperative agreements. While studies were predominantly conducted in the United States, 15.2% were conducted in low- and middle-income countries in Africa, Latin America, and Asia. Adults aged 19–64 years and children aged 2–11 years represented most of the study populations (45.5 and 15.2%, respectively). Studies provided nutrition/dietary guidelines and created culturally tailored interventions, which were then adapted in collaboration with community partners in schools, hospitals, and religious settings. The most cited IS outcomes were feasibility, costs, adoption, and acceptability. Sixteen awards (48.5%) used an IS theory, model, or framework to guide their work.

Discussion

The findings show the breadth of NIH-funded nutrition and implementation research and highlight potential research opportunities.
Kitchen fine particulate matter (PM2.5) concentrations from biomass fuel use in rural households of Northwest Ethiopia
Authors: Enyew Habtamu Demelash , Hailu Abebe Beyene , Mereta Seid Tiku
Published: October 2023

Background

Combustion of solid biomass fuels using traditional stoves which is the daily routine for 3 billion people emits various air pollutants including fine particulate matter which is one of the widely recognized risk factors for various cardiorespiratory and other health problems. But, there is only limited evidences of kitchen PM2.5 concentrations in rural Ethiopia.

Objective

This study is aimed to estimate the 24-h average kitchen area concentrations of PM2.5 and to identify associated factors in rural households of northwest Ethiopia.

Method

The average kitchen area PM2.5 concentrations were measured using a low-cost light-scattering Particle and Temperature Sensor Plus (PATS+) for a 24-h sampling period. Data from the PATS+ was downloaded in electronic form for further analysis. Other characteristics were collected using face-to-face interviews. Independent sample t-test and one-way analysis of variance were used to test differences in PM2.5 concentrations between and among various characteristics, respectively.

Result

Mixed fuels were the most common cooking biomass fuel. The 24-h average kitchen PM2.5 concentrations was estimated to be 405 μg/m3, ranging from 52 to 965 μg/m3. The average concentrations were 639 vs. 336 μg/m3 (p < 0.001) in the thatched and corrugated iron sheet roof kitchens, respectively. The average concentration was also higher among mixed fuel users at 493 vs. 347 μg/m3 (p = 0.042) compared with firewood users and 493 vs. 233 μg/m3 (p = 0.007) as compared with crop residue fuel users. Statistically significant differences were also observed across starter fuel types 613 vs. 343 μg/m3 (p = 0.016) for kerosene vs. dried leaves and Injera baking events 523 vs. 343 μg/m3 (p < 0.001) for baked vs. not baked events.

Conclusion

The average kitchen PM2.5 concentrations in the study area exceeded the world health organization indoor air quality guideline value of 15 μg/m3 which can put pregnant women at greater risk and contribute to poor pregnancy outcomes. Thatched roof kitchen, mixed cooking fuel, kerosene fire starter, and Injera baking events were positively associated with high-level average kitchen PM2.5. concentration. Simple cost-effective interventions like the use of chimney-fitted improved stoves and sensitizing women about factors that aggravate kitchen PM2.5 concentrations could reduce kitchen PM 2.5 levels in the future.
Serious life events and associated PTSD in Gambian girls exposed to female genital cutting
Authors: Heir Trond , Bendiksen Bothild , Minteh Fabakary , Kuye Rex A. , Lien Inger-Lise
Published: October 2023

Introduction

Poor mental health, such as post-traumatic stress disorder (PTSD), has been reported after female genital cutting (FGC). However, data documenting adverse consequences of FGC have insufficiently considered confounding factors, such as other traumatising events. Here, we examined the extent to which FGC versus other serious life events disturbed Gambian girls subjected to FGC. We additionally assessed the prevalence of PTSD and the extent to which it was attributed to FGC versus other serious life events.

Methods

We conducted a cross-sectional study with a community-based sample of 12 years-old Gambian girls who had been subjected to FGC (N = 125). Using structured interviews, we assessed serious life events and probable PTSD related to the event that the girls cited as bothering them the most.

Results

Most of the girls reported several serious life events in addition to FGC, such as witnessing violence, experiencing violence or assaults, death of a close relative, and being exposed to natural disasters or serious accidents, for an average 4.5 events per girl. Around one-sixth of the girls (16.8%) stated that FGC was the event that currently bothered them the most, whereas the majority (75.2%) emphasised other experiences. The girls who said they were most troubled by other events reported more impaired daily functioning than those most bothered by FGC. Overall, we found a prevalence of probable PTSD of 19.2%. Of 24 PTSD cases, one was attributed to the experience of FGC, and the remaining 23 were attributed to other events.

Conclusion

Our findings indicate that FGC is less important than other serious life events in explaining high rates of PTSD in Gambian girls. Associations established in the field between FGC and adverse mental health must be interpreted with caution because girls who have undergone FGC may be severely exposed to other traumatising events.
Bacterial Quorum Sensing: The Most Ancient Language on Earth
Authors: Bassler Bonnie L. , Greenberg E. Peter , Silverman Michael R.
Published: October 2023

A few decades ago, scientists believed that bacteria were very basic creatures that did not communicate with each other and were only good at multiplying. Recently, we have realized that this is far from the truth! Bacteria communicate with one another using a language called quorum sensing. You can think of bacterial quorum sensing as the first-ever social network! In this article, we will tell you about the discovery of quorum sensing and how it radically changed our understanding of the microbial world. We will also tell you how our new knowledge of quorum sensing might help doctors to treat dangerous bacterial infections in humans. Join us in this journey exploring the fascinating language of bacteria and how it could benefit human health.
AI Helping Science: The ‘Shape’ of Things to Come
Authors: Hassabis Demis , Jumper John
Published: October 2023

When we started working with artificial intelligence (AI) more than a decade ago, people were skeptical about whether this technology would develop enough in the foreseeable future to do anything useful. But we held on to our faith in AI’s potential to benefit humanity. We used games like chess, Go and Atari to train and test our AI systems to become smarter and more capable. In 2016, we decided to use our smart systems to try to solve a 50-year-old fundamental problem in biology, called the protein-folding problem. This was the birth of AlphaFold, our AI system that predicts the three-dimensional structures of proteins based on their amino acid sequence. In this article, you will learn about AlphaFold’s achievements, which demonstrate the power of AI to dramatically accelerate scientific discovery and benefit society.
SmartBuild RecSys: A Recommendation System Based on the Smart Readiness Indicator for Energy Efficiency in Buildings
Authors: Siddique Muhammad Talha , Koukaras Paraskevas , Ioannidis Dimosthenis , Tjortjis Christos
Published: October 2023

The Smart Readiness Indicator (SRI) is a newly developed framework that measures a building’s technological readiness to improve its energy efficiency. The integration of data obtained from this framework with data derived from Building Information Modeling (BIM) has the potential to yield compelling results. This research proposes an algorithm for a Recommendation System (RS) that uses SRI and BIM data to advise on building energy-efficiency improvements. Following a modular programming approach, the proposed system is split into two algorithmic approaches linked with two distinct use cases. In the first use case, BIM data are utilized to provide thermal envelope enhancement recommendations. A hybrid Machine Learning (ML) (Random Forest–Decision Tree) algorithm is trained using an Industry Foundation Class (IFC) BIM model of CERTH’S nZEB Smart Home in Greece and Passive House database data. In the second use case, SRI data are utilized to develop an RS for Heating, Ventilation, and Air Conditioning (HVAC) system improvement, in which a process utilizes a filtering function and KNN algorithm to suggest automation levels for building service improvements. Considering the results from both use cases, this paper provides a solid framework that exploits more possibilities for coupling SRI with BIM data. It presents a novel algorithm that exploits these data to facilitate the development of an RS system for increasing building energy efficiency.
Time-Kill Analysis of Canine Skin Pathogens: A Comparison of Pradofloxacin and Marbofloxacin
Authors: Azzariti Stefano , Mead Andrew , Toutain Pierre-Louis , Bond Ross , Pelligand Ludovic
Published: October 2023

Time-kill curves (TKCs) are more informative compared with the use of minimum inhibitory concentration (MIC) as they allow the capture of bacterial growth and the development of drug killing rates over time, which allows to compute key pharmacodynamic (PD) parameters. Our study aimed, using a semi-mechanistic mathematical model, to estimate the best pharmacokinetic/pharmacodynamic (PK/PD) indices (ƒAUC/MIC or %ƒT > MIC) for the prediction of clinical efficacy of veterinary FQs in Staphylococcus pseudintermedius, Staphylococcus aureus, and Escherichia coli collected from canine pyoderma cases with a focus on the comparison between marbofloxacin and pradofloxacin. Eight TCKs for each bacterial species (4 susceptible and 4 resistant) were analysed in duplicate. The best PK/PD index was ƒAUC24h/MIC in both staphylococci and E. coli. For staphylococci, values of 25–40 h were necessary to achieve a bactericidal effect, whereas the calculated values (25–35 h) for E. coli were lower than those predicting a positive clinical outcome (100–120 h) in murine models. Pradofloxacin showed a higher potency (lower EC50) in comparison with marbofloxacin. However, no difference in terms of a maximal possible pharmacological killing rate (Emax) was observed. Taking into account in vivo exposure at the recommended dosage regimen (3 and 2 mg/kg for pradofloxacin and marbofloxacin, respectively), the overall killing rates (Kdrug) computed were also similar in most instances.
Simulating Heavy Rainfall Associated with Tropical Cyclones and Atmospheric Disturbances in Thailand Using the Coupled WRF-ROMS Model—Sensitivity Analysis of Microphysics and Cumulus Parameterization Schemes
Authors: Torsri Kritanai , Faikrua Apiwat , Peangta Pattarapoom , Sawangwattanaphaibun Rati , Akaranee Jakrapop , Sarinnapakorn Kanoksri
Published: October 2023

Predicting heavy rainfall events associated with Tropical Cyclones (TCs) and atmospheric disturbances in Thailand remains challenging. This study introduces a novel approach to enhance forecasting precision by utilizing the coupled Weather Research and Forecasting (WRF) and Regional Oceanic Model (ROMS), known as WRF-ROMS. We aim to identify the optimal combination of microphysics (MP) and cumulus (CU) parameterization schemes. Three CU schemes, namely, Betts-Miller-Janjic (BMJ), Grell 3D Ensemble (G3), and Kain-Fritsch (KF), along with three MP schemes, namely, Eta (ETA), Purdue Lin (LIN), and WRF Single-moment 3-class (WSM3), are selected for the sensitivity analysis. Seven instances of heavy (35.1–90.0 mm) to violent (>90.1 mm) rainfall in Thailand, occurring in 2020 and associated with tropical storms and atmospheric disturbances, are simulated using all possible combinations of the chosen physics schemes. The simulated rain intensities are compared against observations from the National Hydroinformatics Data Center. Performance was assessed using the probability of detection (POD), false alarm ratio (FAR), and critical success index (CSI) metrics. While the models performed well for light (0.1–10.0 mm) to moderate (10.1–35.0 mm) rainfall, forecasting heavy rainfall remained challenging. Certain parameter combinations showed promise, like BMJ and KF with LIN microphysics, but challenges persisted. Analyzing density distribution of daily rainfall, we found effective parameterizations for different sub-regions. Our findings emphasize the importance of tailored parameterizations for accurate rainfall prediction in Thailand. This customization can benefit water resource management, flood control, and disaster preparedness. Further research should expand datasets, focusing on significant heavy rainfall events and considering climate factors, for example, the Madden-Julian Oscillation (MJO) for extended-range forecasts, potentially contributing to sub-seasonal and seasonal (S2S) predictions.
Quantum-Chemical Study of the Benzene Reaction with Fluorine
Authors: Adamson Sergey O. , Kharlampidi Daria D. , Shtyrkova Anastasia S. , Umanskii Stanislav Y. , Dyakov Yuri A. , Morozov Igor I. , Golubkov Maxim G.
Published: October 2023

The reaction of benzene with fluorine atoms may be of interest as a source of phenyl and ipso-fluorocyclohexadienyl radicals or as a method for fluorobenzene gas phase synthesis. The structures and electronic energies of the equilibrium configurations and transition complexes of the C6H6F system are calculated in the density functional approximation. It was found that the interaction of benzene with atomic fluorine can proceed via two channels: hydrogen abstraction with the phenyl radical formation, and hydrogen substitution with the ipso-fluorocyclohexadienyl radical as primary product. Then the dissociation of the ipso-fluorocyclohexadienyl radical leads to creation of fluorobenzene and atomic hydrogen. The initiation of this reaction requires the activation energy near 27 kcal/mol, which indicates the low probability of this process, occurring at temperatures close to the standard (298 K). The calculations of the fluorocyclohexadienyl isomers and their cations also indicate that the formation of fluorobenzene as a product of secondary reactions is unlikely. The conclusions are confirmed by experimental data.
Review of Rydberg Spectral Line Formation in Plasmas
Authors: Letunov Andrey Yu. , Lisitsa Valery S.
Published: October 2023

The present review is dedicated to the problem of an array of transitions between highly-excited atomic levels. Hydrogen atoms and hydrogen-like ions in plasmas are considered here. The presented methods focus on calculation of spectral line shapes. Fast and simple methods of universal ionic profile calculation for the Hnα (Δn=1) and Hnβ (Δn=2) spectral lines are demonstrated. The universal dipole matrix elements formulas for the Hnα and Hnβ transitions are presented. A fast method for spectral line shape calculations in the presence of an external magnetic field using the formulas for universal dipole matrix elements is proposed. This approach accounts for the Doppler and Stark–Zeeman broadening mechanisms. Ion dynamics effects are treated via the frequency fluctuation model. The accuracy of the presented model is discussed. A comparison of this approach with experimental data and the results of molecular dynamics simulation is demonstrated. The kinetics equation for the populations of highly-excited ionic states is solved in the parabolic representation. The population source associated with dielectronic recombination is considered.
Mechanical Characterization of the Human Abdominal Wall Using Uniaxial Tensile Testing
Authors: Kriener Kyleigh , Lala Raushan , Homes Ryan Anthony Peter , Finley Hayley , Sinclair Kate , Williams Mason Kelley , Midwinter Mark John
Published: October 2023

It is generally accepted that the human abdominal wall comprises skin, subcutaneous tissues, muscles and their aponeuroses, and the parietal peritoneum. Understanding these layers and their mechanical properties provides valuable information to those designing procedural skills trainers, supporting surgical procedures (hernia repair), and engineering-based work (in silico simulation). However, there is little literature available on the mechanical properties of the abdominal wall in layers or as a composite in the context of designing a procedural skills trainer. This work characterizes the tensile properties of the human abdominal wall by layer and as a partial composite. Tissues were collected from fresh-never-frozen and fresh-frozen cadavers and tested in uniaxial tension at a rate of 5 mm/min until failure. Stress–strain curves were created for each sample, and the values for elastic moduli, ultimate tensile strength, and strain at failure were obtained. The experimental outcomes from this study demonstrated variations in tensile properties within and between tissues. The data also suggest that the tensile properties of composite abdominal walls are not additive. Ultimately, this body of work contributes to a deeper comprehension of these mechanical properties and will serve to enhance patient care, refine surgical interventions, and assist with more sophisticated engineering solutions.
Extracellular Vesicles as Therapeutic and Diagnostic Tools for Chronic Liver Diseases
Authors: Leszczynska Aleksandra , Stoess Christian , Sung Hana , Povero Davide , Eguchi Akiko , Feldstein Ariel
Published: October 2023

Chronic liver diseases can lead to fibrotic changes that may progress to the development of cirrhosis, which poses a significant risk for morbidity and increased mortality rates. Metabolic dysfunction-associated steatotic liver disease (MASLD), alcohol-associated liver disease (ALD), and viral hepatitis are prevalent liver diseases that may lead to cirrhosis. The advanced stages of cirrhosis can be further complicated by cancer development or end-stage liver disease and liver failure. Hence, early detection and diagnosis of liver fibrosis is crucial for preventing the progression to cirrhosis and improving patient outcomes. Traditionally, invasive liver biopsy has been considered the gold standard for diagnosing and staging liver fibrosis. In the last decade, research has focused on non-invasive methods, known as liquid biopsies, which involve the identification of disease-specific biomarkers in human fluids, such as blood. Among these alternative approaches, extracellular vesicles (EVs) have emerged as promising diagnostic and therapeutic tools for various diseases, including chronic liver diseases. EVs are released from stressed or damaged cells and can be isolated and quantified. Moreover, EVs facilitate cell-to-cell communication by transporting various cargo, and they have shown the potential to reduce the expression of profibrogenic markers, making them appealing tools for novel anti-fibrotic treatments. This review focuses on the impact of EVs in chronic liver diseases and exploring their potential applications in innovative therapeutic and diagnostic approaches.
Update of Potential Biomarkers in Risk Prediction and Monitoring of Atherosclerosis in Systemic Lupus Erythematosus to Prevent Cardiovascular Disease
Authors: Blachut Dominika , Przywara-Chowaniec Brygida , Tomasik Andrzej , Kukulski Tomasz , Morawiec Beata
Published: October 2023

Systemic lupus erythematosus is a chronic connective tissue disease associated with an increased risk of premature atherosclerosis. It is estimated that approximately 10% of SLE patients develop significant atherosclerosis each year, which is responsible for premature cardiovascular disease that is largely asymptomatic. This review summarizes the most recent reports from the past few years on biomarkers of atherosclerosis in SLE, mainly focusing on immune markers. Persistent chronic inflammation of the vascular wall is an important cause of cardiovascular disease (CVD) events related to endothelial dysfunction, cell proliferation, impaired production and function of nitric oxide and microangiopathic changes. Studies on pathogenic immune mediators involved in atherosclerosis will be crucial research avenues for preventing CVD.