bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial Variation in Soil Physico-Chemical Properties along Slope Position in a Small Agricultural Watershed Scale
Authors: Zhu Hansong , Li Haiqiang , Liang Chenglong , Chang Xingchen , Wei Xiaorong , Zhao Xiaoning
Published: October 2022

Both cropland management and slop erosion are important factors influencing soil properties, but there are relatively few studies on their combined effects. Studies at the agricultural watershed scale can satisfy both of these conditions, and to reduce the effects of soil heterogeneity due to differences in spatial scale, it is better to select different slopes in the same small watershed. To understand how soil properties will respond to the variation of slope position and cropland management at an agricultural watershed, we present the distribution of soil bulk density (BD), saturated hydraulic conductivity (Ks), water-stable aggregates, soil organic carbon (OC), nitrogen (N), and phosphorus (P) at four slope positions in two cropland management systems of a small agricultural watershed in the black soil region of northeast China. The selected four slope positions include upper slope, middle slope, lower slope, and footslope positions. The two cropland management systems consist of a sustainable cropland system (i.e., contour ridge tillage at upper slope position, longitudinal ridge tillage at middle slope and lower slope positions, and grassland at footslope position) and a conventional cropland system (i.e., contour ridge tillage at upper slope position, longitudinal ridge tillage at middle slope, lower slope positions, and footslope positions). The results showed that soil bulk density and microaggregates decreased but the concentration of OC and nutrients, Ks, and small-macroaggregate increased from the upper slope position to the lower slope position in both the conventional and sustainable croplands, which was due to the interaction effect of cultivation with erosion. In comparison with conventional cropland, sustainable cropland has greater Ks, large-macroaggregate, small-macroaggregate, microaggregate, and concentrations and stocks of OC, N, and P, but showed lower bulk density and silt + clay fraction. However, the prominent differences in both croplands were presented in the footslope position, which is ascribed to the interaction of cultivation, erosion, and cropland management. These results highlighted that sustainable cropland management practice has the potential to improve soil structure and prevent soil and nutrient loss.
Screening of Heat-Tolerant Indica Rice Varieties in the Middle and Lower Yangtze River
Authors: Zhang Man , Feng Kaixuan , Dong Xinde , Zhou Yongjin , Chen Jinhua , Teng Bin , Li Zhong , Ren Lantian , Wu Wenge
Published: October 2022

Rice growth is constantly challenged by severe environmental conditions, especially high-temperature stress, brought about by global warming. How to increase rice yields is a current issue in many countries. By analyzing the meteorological data from Anhui Province over the past 60 years, the frequency and level of high-temperature damage were comprehensively analyzed. On this basis, five indica rice varieties with strong high-temperature tolerance, relatively high yield and stable yield were selected by laboratory-simulated high-temperature and field high-temperature experiments: Longliangyou Huazan (LLYHZ), 9you-6 (9Y-6), Longliangyou-1988 (LLY-1988), Fengliangyou-4 (FLY-4) and Huanghuazan (HHZ). These are suitable for planting in the middle and lower reaches of the Yangtze River. This study provides a theoretical reference and scientific basis for the study of rice tolerance to high-temperature stress.
Full-Length Genome of the Equine Influenza A Virus Subtype H3N8 from 2019 Outbreak in Saudi Arabia
Authors: Alaql Fanan A. , Alhafufi Ali N. , Kasem Samy , Alhammad Yousef M. O. , Albaqshi Hassan , Alyousaf Ameen , Alsubaie Faisal M. , Alghamdi Ahmed N. , Abdel-Moneim Ahmed S. , Alharbi Sulaiman A.
Published: October 2022

Equine influenza is a major cause of respiratory infections in horses and can spread rapidly despite the availability of commercial vaccines. This study aimed to screen the incidence of equine influenza virus (EIV) and molecularly characterize the haemagglutinin and neuraminidase from positive EIV field samples collected from Saudi Arabia. Six-hundred twenty-one horses from 57 horse barns were screened for the presence of the clinical signs, suggestive for equine influenza, from different parts of Saudi Arabia. Nasopharyngeal swabs were collected from each horse showing respiratory distress. Samples from the same horse barn were pooled together and screened for the presence of the influenza A virus using quantitative real time reverse transcriptase polymerase chain reaction (qRT-PCR). Selective positive samples were subjected to full-length genome sequencing using MiSeq Illumina. Out of the total 57 pools, 39 were found positive to EIV using qRT-PCR. Full-length gene sequences were compared with representative EIV strains selected from the GenBank database. Phylogenetic analysis of the HA and NA genes revealed that the identified virus strains belong to H3N8 clade 1 of the Florida sublineage and were very similar to viruses identified in USA in 2019, with no current evidence for reassortment. This is one of the first reports providing detailed description and characterization of EIVs in Saudi Arabia. Detailed surveillance and genetic information sharing could allow genetic evolution of equine influenza viruses to be monitored more effectively on a global basis and aid in refinement of vaccine strain selection for EIV.
Serum Analytes of American Mink (Neovison Vison) Challenged with Aleutian Mink Disease Virus
Authors: Farid A. Hossain , Rupasinghe Priyanka P.
Published: October 2022

Black American mink (Neovison vison), which had been selected for tolerance to Aleutian mink disease virus (AMDV) for more than 20 years (TG100) or were from herds that have been free of AMDV (TG0), along with their progeny and crosses with 50% and 75% tolerance ancestry, were inoculated with a local isolate of AMDV. Blood samples were collected from 493 mink between 120 and 1211 days post-inoculation, and concentrations of 14 serum analytes were measured. Distributions of all analytes significantly deviated from normality, and data were analyzed after Box–Cox power transformation. Significant differences were observed among tolerant groups in the concentrations of globulin (GLO), total protein (TP), alkaline phosphatase, urea nitrogen, and calcium. Concentrations of GLO and TP linearly and significantly decreased with an increasing percentage of tolerance ancestry. Eleven analytes had the smallest values in the tolerant groups (TG100 or TG75), and eight analytes had the greatest values in the non-selected groups (TG0 or TG50). Antibody titer had the greatest correlation coefficients with GLO (0.62), TP (0.53), and creatinine (0.36). It was concluded that selection for tolerance decreased the concentrations of most serum analytes, and TP and GLO were the most accurate biomarkers of tolerance to AMDV infection. Males had significantly greater values than females for phosphorus and total bilirubin concentrations, but females had significantly greater amylase, cholesterol, and BUN concentrations than males.
Investigation of Parasitic Infection in Crocodile Lizards (Shinisaurus crocodilurus) Using High-Throughput Sequencing
Authors: Zeng Yongru , Xiong Yi , Yang Chunsheng , He Nan , He Jiasong , Luo Wenxian , Chen Yaohuan , Zeng Xiaochen , Wu Zhengjun
Published: October 2022

The highly endangered crocodile lizard (Shinisaurus crocodilurus) continues to be impacted by disease, especially in captive breeding populations. In this paper, based on high-throughput sequencing, we investigated parasitic infections in captive and wild crocodile lizard populations in the Daguishan National Nature Reserve and Guangdong Luokeng Shinisaurus crocodilurus National Nature Reserve. The results show that the overall parasitic infection rate in crocodile lizards was 33.33% (23/69). Four parasite genera were detected, including Eimeria, Cryptosporidium, Nematopsis, and Acanthamoeba, with infection rates of 15.94% (11/69), 17.39% (12/69), 7.25% (5/69), and 4.35% (3/69), respectively. Significant differences in the infection rate were found between the different parasite species (χ2 = 8.54, p < 0.05, chi-squared test). The parasitic infection rates in the captive and wild populations were 39.29% (22/56) and 7.69% (1/13), respectively, which were significantly different (p < 0.05, Fisher’s exact test). However, no significant differences in the infection rates of the four parasite genera were found between the captive and wild populations (p > 0.05, Fisher’s exact test). The parasitic infection rates in Daguishan and Luokeng were 34.09% (15/44) and 32.00% (8/25), respectively, which were not significantly different (p > 0.05, Fisher’s exact test). However, significant differences in terms of species were found in the two reserves (p < 0.01, Fisher’s exact test). Only Cryptosporidium infection showed a significant difference between the two regions (p < 0.01, Fisher’s exact test). Our results suggest that captive crocodile lizards are more susceptible to parasitic diseases than wild crocodile lizards and that Cryptosporidium infection varies by geographical region. This study provides basic information about the parasites of endangered crocodile lizards, as well as a reference for disease control and conservation.
Unexpected Candidal Hyphae in Oral Mucosa Lesions—A Clinico-Pathological Study
Authors: Edel Jeremy , Grinstein-Koren Osnat , Winocur-Arias Orit , Kaplan Ilana , Schnaiderman-Shapiro Anna , Buchner Amos , Vered Marilena , Zlotogorski-Hurvitz Ayelet
Published: October 2022

Background: Oral mucosal biopsies might harbor candidal hyphae (CH) in the absence of any clinical signs or symptoms. Aim: To assess oral mucosa biopsies for the frequency of unexpected CH and characterize their clinico-pathological features. Materials and Methods: All biopsy reports (2004–2019) were searched using CH/candida/candidiasis as key words. Cases with clinical diagnosis of oral candidiasis (OC) were excluded. Demographic data, health status, smoking habits, clinical features and diagnoses were collected. Statistical analysis included the chi-square test; significance was set at p < 0.05. Results: Of all the biopsies, 100 (1.05%) reported microscopical evidence of CH without typical clinical signs/symptoms of OC. Fifteen cases were from healthy, non-smoking patients. CH was common on buccal mucosa (38%) and lateral tongue (23%). The tip of tongue (OR = 54.5, 95% CI 9.02–329.4, p < 0.001) and lateral tongue (OR = 3.83, 95% CI 2.4–6.09, p < 0.001) were more likely to harbor CH-positive lesions. CH-positive lesions were diagnosed as epithelial hyperplasia (55%) and exophytic reactive lesions (30%). No correlation was found between CH and the grade of epithelial dysplasia. Conclusions: Microscopic evidence of CH embedded into oral epithelium without typical signs/symptoms of OC is rare, especially in healthy, non-smokers. Since CH was occasionally found in oral sites prone to local trauma and in association with reactive lesions, in absence of host co-morbidities, the contribution of local mechanical forces to CH embedment cannot be ruled out.
Influence of Citrus Essential Oils on the Microbiological, Physicochemical and Antioxidant Properties of Primosale Cheese
Authors: Busetta Gabriele , Ponte Marialetizia , Barbera Marcella , Alfonzo Antonio , Ioppolo Antonino , Maniaci Giuseppe , Guarcello Rosa , Francesca Nicola , Palazzolo Eristanna , Bonanno Adriana , Moschetti Giancarlo , Settanni Luca , Gaglio Raimondo
Published: October 2022

The aim of this study was to produce a fresh ovine pressed cheese within Pecorino “Primosale” typology with the addition of citrus essential oils (EOs). For this purpose, ewe’s pasteurized milk was added with EOs from the peel of lemons, oranges and tangerines. Seven cheese productions were performed at the pilot plant scale level, including one control production (CP) without the addition of EOs and six experimental productions obtained by the addition of two EO concentrations (100 and 200 µL/L) to milk. The acidification process was obtained by means of the starter cultures Lactococcus lactis CAG4 and PON36. All cheeses showed levels of lactic acid bacteria (LAB) around 109 CFU/g, indicating that citrus EOs did not negatively influence the starter evolution. The addition of citrus EOs did not determine significant variations for dry matter, fat and protein percentages but increased the antioxidant capacity of all the experimental cheeses of about 50% in comparison to the control trial. The citrus EOs impacted cheese VOCs, especially for terpene class (limonene, β-pinene, myrcene, carene, linalool and α-terpineol). The sensory evaluation showed that cheeses enriched with 100 µL/L of citrus EOs were mostly appreciated by the panelists.
Power System State Estimation Approach Considering Transmission Line Temperature
Authors: Xu Qingwen , Zhang Hengxu , Cao Yongji , Qin Hao , Gao Zhimin
Published: October 2022

The transmission line parameters vary with the change of temperature, which has a significant impact on power system state estimation (SE). Based on the theory of electro-thermal coordination (ETC), this paper proposes two ETC-SE approaches with the consideration of transmission line temperature. The heat balance equation (HBE) is combined with the conventional weighted least square SE for establishing an ETC-SE model. Moreover, an augmented Jacobian ETC-SE approach is developed by integrating the HBE into pseudo measurements and the line temperature into state vectors. The Jacobian matrix is augmented correspondingly and the partial differential coefficients of measurements to line temperature are provided, which enables to calculate line temperature and voltage phasors simultaneously. Furthermore, in order to accelerate the solving process, an improved two-step ETC-SE algorithm is proposed, in which the SE and temperature estimation are decoupled and solved via alternate iteration. The effectiveness of the proposed ETC-SE approaches is verified by the IEEE 14-, 39-, and 118-bus systems. The results show that the proposed ETC-SE approach is effective to reduce the calculation errors and possesses good convergence performance with varying environmental circumstances and ill-conditioned branches.
EtWExplorer: Multi-Priority Scheduling Path Exploration Technology Based on Abstract Syntax Tree Analysis
Authors: He Xinglu , Wang Pengfei , Lu Kai , Zhou Xu
Published: October 2022

Symbolic execution is well known as a dynamic vulnerability discovery technique. Its greatest advantage is the capability to analyze the execution information of the program and to explore the path in the program deterministically. This is a more accurate way to determine if there are vulnerabilities in a program than randomized testing by fuzzing. In addition, symbolic execution does not suffer from the problem of decreasing the capability to discover new paths as more paths are discovered, similar to that caused by random-based fuzzing. However, the reason why symbolic execution is not widely used in vulnerability discovery is mainly due to the state space explosion in the program. The state space explosion severely affects the applicability of symbolic execution. To further improve the applicability of symbolic execution, this paper proposes a path exploration technology based on abstract syntax tree analysis. With the distance between the expression generated by the symbolic execution of the repeat location and the “unsatisfiable” condition of the “unsat” state, we can perform multi-priority scheduling for the repeat location state, thus mitigating the impact of the state space explosion on path exploration. We proposed and implemented EtWExplorer, a multi-priority scheduling technique based on abstract syntax tree analysis. With this technique, we can significantly improve the capability of symbolic execution to discover unknown paths even in state space exploration. Experiments show that EtWExplorer introduces a performance overhead of 72% in the worst case and can improve performance by 294% in the best case. EtWExplorer has a 95% improvement in state space explosion mitigation capability and a 199% to 983% improvement in the path exploration capability of block coverage and a 181% to 1047% improvement in the path exploration capability of edge coverage when facing programs that cause a state space explosion.
The New Improved ZHD and Weighted Mean Temperature Models Based on GNSS and Radiosonde Data Using GPT3 and Fourier Function
Authors: Li Li , Gao Ying , Xu Siyi , Lu Houxian , He Qimin , Yu Hang
Published: October 2022

Compared to the zenith hydrostatic delay (ZHD) obtained from the Saastamonien model based on in-situ measured meteorological (IMM) data and radiosonde-derived weighted mean temperature (Tm), the ZHD and Tm deviations of the GPT3 model have shown obvious periodic trends. This article analyzed the seasonal variations of GPT3-ZHD and GPT3-Tm during the 2016–2020 period in the Yangtze River Delta region, and the new improved ZHD and Tm models were established by the multi-order Fourier function. The precision of the improved-ZHD model was verified using IMM-ZHD products from 7 GNSS stations during the 2016–2020 period. Furthermore, the precisions of improved Tm and precipitable water vapor (PWV) were verified by radiosonde-derived Tm and PWV in the 2016–2019 period. Compared with the IMM-ZHD and GNSS-PWV products, the mean Bias and RMS of GPT3-ZHD are −0.5 mm and 2.1 mm, while those of GPT3-PWV are 2.7 mm and 11.1 mm. Compared to the radiosonde-derived Tm, the mean Bias and RMS of GPT3-Tm are −0.8 K and 3.2 K. The mean Bias and RMS of the improved-ZHD model from 2019 to 2020 are −0.1 mm and 0.5 mm, respectively, decreasing by 0.4 mm and 1.6 mm compared to the GPT3-ZHD, while those of the improved-Tm are −0.6 K and 2.7 K, respectively, decreasing by 0.2 K and 0.5 K compared to GPT3-Tm. The mean Bias and RMS of PWV calculated by GNSS-ZTD, improved-ZHD, and improved-Tm are 0.5 mm and 0.6 mm, respectively, compared to the GNSS-PWV, decreasing by 2.2 mm and 10.5 mm compared to the GPT3-PWV. It indicates that the improved ZHD and Tm models can be used to obtain the high-precision PWV. It can be applied effectively in the retrieval of high-precision PWV in real-time in the Yangtze River Delta region.
Enhancing the Production of Pinene in Escherichia coli by Using a Combination of Shotgun, Product-Tolerance and I-SceI Cleavage Systems
Authors: Huang Ming-Yue , Wang Wei-Yang , Liang Zhen-Zhen , Huang Yu-Chen , Yi Yi , Niu Fu-Xing
Published: October 2022

Tolerance breeding through genetic engineering, sequence and omics analyses, and gene identification processes are widely used to synthesize biofuels. The majority of related mechanisms have been shown to yield endogenous genes with high expression. However, the process was time-consuming and labor-intensive, meaning there is a need to address the problems associated with the low-throughput screening method and significant time and money consumption. In this study, a combination of the limit screening method (LMS method) and product-tolerance engineering was proposed and applied. The Escherichia coli MG1655 genomic DNA library was constructed using the shotgun method. Then, the cultures were incubated at concentrations of 0.25%, 0.5%, 0.75% and 1.0% of pinene with different inhibitory effects. Finally, the genes acrB, flgFG, motB and ndk were found to be associated with the enhanced tolerance of E. coli to pinene. Using the I-SceI cleavage system, the promoters of acrB, flgFG and ndk genes were replaced with P37. The final strain increased the production of pinene from glucose by 2.1 times.
Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression
Authors: Pang Yaoyu , Maxwell Ellen , Sindrewicz-Goral Paulina , Shapanis Andrew , Li Shun , Morgan Mark , Yu Lu-Gang
Published: October 2022

Melanoma cell adhesion molecule (MCAM, CD146, MUC18) is a heavily glycosylated transmembrane protein and a marker of melanoma metastasis. It is expressed in advanced primary melanoma and metastasis but rarely in benign naevi or normal melanocytes. More and more evidence has shown that activation of the MCAM on cell surface plays a vital role in melanoma progression and metastasis. However, the natural MCAM binding ligand that initiates MCAM activation in melanoma so far remains elusive. This study revealed that galectin-3, a galactoside-binding protein that is commonly overexpressed in many cancers including melanoma, is naturally associated with MCAM on the surface of both skin and uveal melanoma cells. Binding of galectin-3 to MCAM, via O-linked glycans on the MCAM, induces MCAM dimerization and clustering on cell surface and subsequent activation of downstream AKT signalling. This leads to the increases of a number of important steps in melanoma progression of cell proliferation, adhesion, migration, and invasion. Thus, galectin-3 is a natural binding ligand of MCAM in melanoma, and their interaction activates MCAM and promotes MCAM-mediated melanoma progression. Targeting the galectin-3–MCAM interaction may potentially be a useful therapeutic strategy for melanoma treatment.
Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice
Authors: Lim Gahyun , Lee Heaji , Lim Yunsook
Published: October 2022

Recently, it has been demonstrated that in sarcopenic obesity (SO), physical activity could improve cognitive functions. Moreover, previous studies suggested that muscle contraction could influence cognitive function via myokines. This study investigated the potential effects of resistant exercise on cognitive and muscle functions in SO. SO was induced by a high-fat diet treatment for 8 weeks in 8-month-old male C57BL/6J mice. Then, resistant exercise (ladder climbing) for 8 weeks was performed. Muscle and cognitive function tests and morphological analysis were conducted. The protein levels of myokines were investigated in muscle, plasma, and the hippocampus in sarcopenic obese mice. Muscle and cognitive functions were significantly elevated in the obesity-exercise group (EX) compared to the obesity-control group (OB). Interestingly, muscle function was positively correlated with cognitive function. Abnormal morphological changes in the hippocampus were ameliorated in EX compared to OB, but not in the muscle. Protein levels of cognitive function-related myokines and energy metabolism-related markers in EX were significantly elevated in both muscle and hippocampus compared to those in OB. Interestingly, the protein level of brain-derived neurotrophic factor (BDNF) in EX was simultaneously increased in all tissues including muscle, plasma, and hippocampus compared to that in OB. In conclusion, modulation of muscle-derived cognitive function-related myokines in various pathological conditions via a resistant exercise could be a possible way of relieving muscle and cognitive dysfunction.
Sectional Model Wind Tunnel Test and Research on the Wind-Induced Vibration Response of a Curved Beam Unilateral Stayed Bridge
Authors: Zhao Shuang , Chen Jiahao , Yue Jiahao , Yan Zhitao , Liu Jun , Zhang Bin , Chen Jianfeng
Published: October 2022

The linear curve distribution of the beam and the asymmetrical layout of the stay cables may have beneficial or adverse influences on cable-stayed bridges. Sectional model wind tunnel tests and numerical simulations were used to analyze the influence of these two factors on the wind-induced vibration characteristics of a curved beam unilateral stayed bridges (CBUSB) and the interaction between its stay cables and curved beams. According to the basic similarity law, the sectional models of a CBUSB example were designed and manufactured. The aerodynamic force and wind-induced vibration of the models were measured in an atmospheric boundary wind tunnel laboratory to obtain the aerodynamic coefficient and displacement, respectively. Based on the wind tunnel test results, the verified finite element model was used to determine the displacement, acceleration, and cable tension of the CBUSB excited by the buffeting force under 5 curvature cases and 4 cable layout cases. Then, band-pass filter technology and fast Fourier transform technology were used to analyze the influence of these two parameters on the wind-induced vibration characteristics of the CBUSB. Results show that the CBUSB had good aerodynamic stability in the wind tunnel at low and high wind speeds. With increasing curvature, the high-order modal vibration and modal coupling vibration of the CBUSB may be generated. The frequency, the proportion of wind-induced vibration response components, and the distribution characteristics of spectrum energy of CBUSB will be affected by 4 cable layout schemes. Cables arranged on both sides of the bridge and near the center of curvature can improve pedestrian comfort and reduce wind-induced vibration, respectively. Affected by the interaction between cable and bridge, the cable and bridge transmit their own vibration to each other, both of which contain the response components of each other.
An Assessment of the Relation between Architectural and Structural Systems in the Design of Tall Buildings in Turkey
Authors: Özşahin Burak
Published: October 2022

Many professionals from several disciplines need to cooperate in designing and constructing tall buildings since their design and construction require more complex systems and technologies in terms of structure, installation, facade, vertical circulation and fire systems compared to low-rise buildings. The architects who design tall buildings have to know the architectural and structural design considerations of tall buildings and their interrelations well. This study is expected to reveal the status of tall buildings completed in Turkey and help designers understand architectural forms, floor plans, core planning and structural systems of tall buildings. For this purpose, the factors affecting architectural and structural design were examined, and the relation between the architectural–structural system was revealed for tall buildings completed in Turkey. In order to study architectural and structural considerations, 230 constructed tall buildings in Turkey were selected and analyzed in detail. According to the results of the study, it is seen that the prismatic form is widely used as the building tower form in Turkey, the shear-frame system as the structural system, the residential function as the building function, the single symmetrical central core as the building core, and the rectangular plan as the building floor plan alike.
Percutaneous Thermal Ablation for Renal Tumors in Patients with Birt–Hogg–Dubé Syndrome
Authors: Bodard Sylvain , Boudhabhay Idris , Dariane Charles , Delavaud Christophe , Guinebert Sylvain , Joly Dominique , Timsit Marc-Olivier , Mejean Arnaud , Verkarre Virginie , Hélénon Olivier , Richard Stéphane , Correas Jean-Michel
Published: October 2022

BHD syndrome is characterized by an increased risk of bilateral and multifocal renal cell carcinoma (RCCs), but is rarely metastatic. Our report aims to analyze the outcome of patients with BHD syndrome who underwent percutaneous thermal ablation (TA). The present report included six BHD syndrome patients (five men) with a mean age of 66 ± 11 (SD) years who had a proven germline FLCN gene mutation and underwent TA for a renal tumor. Nineteen renal tumors (median two tumors per patient; range: 1–3), including seven chromophobe RCCs, five clear-cell RCCs, four papillary RCCs, two clear-cell papillary RCC, and one hybrid oncocytic/chromophobe tumor were treated in 14 ablation sessions. The mean size of the tumors was 21 ± 11 (SD) mm (median: 20 mm; interquartile range (IQR): 14–25 mm) for a mean volume of 7 ± 11 (SD) mL (median: 3; IQR: 1–5 mL). Technical success was achieved in all ablation sessions (primary success rate, 100%). The procedure was well tolerated under conscious sedation with no significant Clavien–Dindo complication (grade 2, 3, 4). All patients were alive with no distant metastasis during a median follow-up period of 74 months (range: 33–83 months). No local tumor progression was observed. The mean decrease in estimated glomerular filtration rate was 8 mL/min/1.73 m2. No patients required dialysis or renal transplantation. In this case series, percutaneous TA appeared as a safe and efficient nephron-sparing treatment for treating RCCs associated with BHD syndrome, even in the case of advanced chronic kidney disease.
Activation of PPARα Ameliorates Cardiac Fibrosis in Dsg2-Deficient Arrhythmogenic Cardiomyopathy
Authors: Qiu Zirui , Zhao Yawen , Tao Tian , Guo Wenying , Liu Ruonan , Huang Jingmin , Xu Geyang
Published: October 2022

Background: Arrhythmogenic cardiomyopathy (ACM) is a genetic heart muscle disease characterized by progressive fibro-fatty replacement of cardiac myocytes. Up to now, the existing therapeutic modalities for ACM are mostly palliative. About 50% of ACM is caused by mutations in genes encoding desmosomal proteins including Desmoglein-2 (Dsg2). In the current study, the cardiac fibrosis of ACM and its underlying mechanism were investigated by using a cardiac-specific knockout of Dsg2 mouse model. Methods: Cardiac-specific Dsg2 knockout (CS-Dsg2−/−) mice and wild-type (WT) mice were respectively used as the animal model of ACM and controls. The myocardial collagen volume fraction was determined by histological analysis. The expression levels of fibrotic markers such as α-SMA and Collagen I as well as signal transducers such as STAT3, SMAD3, and PPARα were measured by Western blot and quantitative real-time PCR. Results: Increased cardiac fibrosis was observed in CS-Dsg2−/− mice according to Masson staining. PPARα deficiency and hyperactivation of STAT3 and SMAD3 were observed in the myocardium of CS-Dsg2−/− mice. The biomarkers of fibrosis such as α-SMA and Collagen I were upregulated after gene silencing of Dsg2 in HL-1 cells. Furthermore, STAT3 gene silencing by Stat3 siRNA inhibited the expression of fibrotic markers. The activation of PPARα by fenofibrate or AAV9-Pparα improved the cardiac fibrosis and decreased the phosphorylation of STAT3, SMAD3, and AKT in CS-Dsg2−/− mice. Conclusions: Activation of PPARα alleviates the cardiac fibrosis in ACM.
Shikonin Inhibits Fin Regeneration in Zebrafish Larvae
Authors: Cao Zigang , Guo Chen , Chen Guilan , Liu Jiejun , Ni Huiwen , Liu Fangsheng , Xiong Guanghua , Liao Xinjun , Lu Huiqiang
Published: October 2022

Shikonin is a naphthoquinone compound extracted from Chinese comfrey for treating cancer. However, there are few reports on its research on vertebrate tissue regeneration. Zebrafish is an ideal model for studying organ regeneration. In this study, we found that 3-dpf of zebrafish larvae exposed to shikonin at concentrations of 0.2, 0.3, and 0.4 mg/L showed increasingly inhibited regeneration of the tail fin. Immunohistochemical staining showed that shikonin exposure from 6 to 12 hpa increased the number of apoptotic cells in the caudal fin wound of larvae and decreased the number of proliferating cells. Shikonin exposure was found to up-regulate oxidative stress, increase ROS levels, and reduce neutrophil recruitment in the early stage of wound repair. Moreover, shikonin exposure caused disordered expression of fin regeneration blastemal-related genes. The use of astaxanthin to down-regulate oxidative stress was found to significantly reduce the inhibition of caudal fin regeneration. Mixed exposure of AMPK inhibitors or fullerenes (C60) with shikonin also showed the similar rescue effect. Collectively, our study showed that shikonin inhibited fin regeneration in zebrafish larvae by the upregulation of oxidative stress level and AMPK signaling pathway. This research provides valuable information on the mechanism of action of shikonin for its safe application.
PANI-Based Stacked Ferromagnetic Systems: Electrochemical Preparation and Characterization
Authors: Stefani Andrea , Fonollosa Natàlia Tanaka , Giurlani Walter , Giovanardi Roberto , Fontanesi Claudio
Published: October 2022

In this work, the electropolymerization of polyaniline (PANI) is explored for its action as either a suitable coating or as a substrate for Nickel (Ni) and Magnetite (Fe3O4) surfaces. PANI electropolymerization has been achieved through cyclic voltammetry (CV), potentiostatic and galvanostatic electrochemical methods. The interaction between the obtained surfaces and the ferromagnetic layers (Ni and Fe3O4) has been investigated as a function of the pH of the electrolytic PANI solution, and also a variety of experimental parameters have been optimized in order to achieve the synthesis of PANI coatings (solvent, substrate, concentrations, and cell set-up). Thus, we obtained stable and consistent PANI thick films at the interface of both the nickel and the magnetite ferromagnetic materials.
Effects of Preparation and Self-Assembly of Poly(Styrene-Acrylic Acid) (P(St-AA)) Microspheres upon Constructed Photonic Crystals with Structural Color on Wood Surfaces
Authors: Hu Jing , Liu Yi , Xu Wei , Wu Zhihui , Pang Xiaoren
Published: October 2022

Colloidal microspheres could be used as raw materials to construct the photonic crystal modification layers on wood surfaces, and the wood would produce gorgeous structural color effect. The diameter, monodispersity and self-assembly of colloidal microspheres directly affect the well-structure order of photonic crystals. Therefore, poly(styrene-acrylic acid) (P(St-AA)) shell-core microspheres were prepared by emulsion polymerization. The effects of the reaction time, amount of initiator and emulsifier and reaction temperature on the particle size and dispersity of colloidal microspheres during polymerization were analyzed by single-factor experiments. The P(St-AA) colloidal microspheres self-assembled on wood surfaces and formed photonic crystals with structural color through thermally-assisted gravity deposition. The effects of the amount of emulsion coating per unit surface area and ambient temperature on the optical properties of the photonic crystals were investigated. It was found that the particle size of the synthesized microspheres increased with the increase of initiator amount and reaction temperature and the decrease of emulsifier; however, the effect of reaction time on microspheres was less regular. The best optical properties of the photonic crystals on wood surfaces were obtained using 0.408 μL/mm2 emulsion coating at a temperature of 50 °C. The photonic crystals were composed of both ordered and disordered layers.
Bird Communities in a Changing World: The Role of Interspecific Competition
Authors: Guillaumet Alban , Russell Ivory Jordan
Published: October 2022

Significant changes in the environment have the potential to affect bird species abundance and distribution, both directly, through a modification of the landscape, habitats, and climate, and indirectly, through a modification of biotic interactions such as competitive interactions. Predicting and mitigating the consequences of global change thus requires not only a sound understanding of the role played by biotic interactions in current ecosystems, but also the recognition and study of the complex and intricate effects that result from the perturbation of these ecosystems. In this review, we emphasize the role of interspecific competition in bird communities by focusing on three main predictions derived from theoretical and empirical considerations. We provide numerous examples of population decline and displacement that appeared to be, at least in part, driven by competition, and were amplified by environmental changes associated with human activities. Beyond a shift in relative species abundance, we show that interspecific competition may have a negative impact on species richness, ecosystem services, and endangered species. Despite these findings, we argue that, in general, the role played by interspecific competition in current communities remains poorly understood due to methodological issues and the complexity of natural communities. Predicting the consequences of global change in these communities is further complicated by uncertainty regarding future environmental conditions and the speed and efficacy of plastic and evolutionary responses to fast-changing environments. Possible directions of future research are highlighted.
Prediction of the Presence of Targetable Molecular Alteration(s) with Clinico-Metabolic 18 F-FDG PET Radiomics in Non-Asian Lung Adenocarcinoma Patients
Authors: Aide Nicolas , Weyts Kathleen , Lasnon Charline
Published: October 2022

This study aimed to investigate if combining clinical characteristics with pre-therapeutic 18 F-fluorodeoxyglucose (18 F-FDG) positron emission tomography (PET) radiomics could predict the presence of molecular alteration(s) in key molecular targets in lung adenocarcinoma. This non-interventional monocentric study included patients with newly diagnosed lung adenocarcinoma referred for baseline PET who had tumour molecular analyses. The data were randomly split into training and test datasets. LASSO regression with 100-fold cross-validation was performed, including sex, age, smoking history, AJCC cancer stage and 31 PET variables. In total, 109 patients were analysed, and it was found that 63 (57.8%) patients had at least one molecular alteration. Using the training dataset (n = 87), the model included 10 variables, namely age, sex, smoking history, AJCC stage, excessKustosis_HISTO, sphericity_SHAPE, variance_GLCM, correlation_GLCM, LZE_GLZLM, and GLNU_GLZLM. The ROC analysis for molecular alteration prediction using this model found an AUC equal to 0.866 (p < 0.0001). A cut-off value set to 0.48 led to a sensitivity of 90.6% and a positive likelihood ratio (LR+) value equal to 2.4. After application of this cut-off value in the unseen test dataset of patients (n = 22), the test presented a sensitivity equal to 90.0% and an LR+ value of 1.35. A clinico-metabolic 18 F-FDG PET phenotype allows the detection of key molecular target alterations with high sensitivity and negative predictive value. Hence, it opens the way to the selection of patients for molecular analysis.
A Sense of Belonging: The People and Counterspaces Latinx Undocu/DACAmented Collegians Use to Persist
Authors: Santa-Ramirez Stephen
Published: October 2022

Guided by sense of belonging and counterspaces, this critical ethnographic study investigates the people, places, and spaces collegians that are Latinx and undocu/DACAmented use to persist toward graduation amidst an ongoing anti-im/migrant sociopolitical climate. Findings reveal that (a) connections built with peers who share racial backgrounds and have liminal legal statuses, (b) supportive and affirming faculty, (c) access to culturally-based student organizations and academic programs, and (d) campus departments and programs catered to the holistic support of undocu/DACAmented collegians are salient for these students’ sense of belonging in college, though belongingness is not fully attainable in the United States as a result of racist nativism. Recommendations for research and practice are offered for higher education institutional agents at all levels.
A Five-Level RF-PWM Method with Third and Fifth Harmonic Elimination for All-Digital Transmitters
Authors: Fu Haoyang , Zhou Qiang , Zhu Lei , Chen Zhang , Wei Zhihu , Zeng Siyu
Published: October 2022

An appropriate pulse-coding algorithm is the key to achieving an efficient switched-mode power amplification in all-digital transmitters. A five-level RF-PWM method with third and fifth harmonic elimination is proposed to relax the requirements of the filter and to reduce the control complexity of the SMPA for all-digital transmitters. By controlling the pulse width and the center position of three-level sub-pulses, third and fifth harmonic elimination is achieved. Meanwhile, the control complexity of the SMPA is reduced by the decrease in the output-signal-level number. Finally, the feasibility of the method is verified by simulation. For the 16QAM signal with a carrier frequency of 200 MHz, the proposed method can achieve third harmonic suppression of −46.24 dBc and fifth harmonic suppression of −54.05 dBc when coding efficiency reaches 77.51%.
Technical, Economic, and Intelligent Optimization for the Optimal Sizing of a Hybrid Renewable Energy System with a Multi Storage System on Remote Island in Tunisia
Authors: Hajjaji Mohamed , Mezghani Dhafer , Cristofari Christian , Mami Abdelkader
Published: October 2022

Due to their small dimension and isolated energy systems, islands face a significant energy supply challenge. In general, they use fossil fuels for electricity generation. Fossil fuels are a major source of CO2 emissions, and they are very costly. The cost of electricity generation on islands is up to 10 times higher than on the mainland. This situation without a doubt represents a financial burden for the islanders. Using renewable sources, especially solar and wind sources, offers great potential for power generation in remote locations, as they are a clean and inexhaustible source of energy. Electrifying these zones with a hybrid system consisting of a photovoltaic (PV) and wind systems associated to a hydraulic and an electrochemical storage system is a promising alternative. The purpose of this study is to optimize the dimension of the components generation of systems, especially for a remote island in Tunisia. The first part of this object outlines the PV-wind-battery-hydraulic generation system architecture and modeling. The optimal sizing of the device additives, satisfying two criteria with the aid of evolutionary algorithms NSGAII, is defined inside the second part of this article. The outcomes are discussed from the point of view of the importance of the system dimension and in terms of compliance with the criteria through the study of the most optimal particular configurations.
Heat Storage and Release Performance of Cascade Phase Change Units for Solar Heating in a Severe Cold Region of China
Authors: Zhang Li , Liu Zhihui , Jin Guang , Cuce Erdem , Jin Jing , Guo Shaopeng
Published: October 2022

The heat storage and release performance of cascade phase change units are investigated numerically for users in Inner Mongolia’s severe cold region. Three schemes of phase change material combinations are thoroughly tested. We obtained a better material combination scheme S3 (palmitic acid + polyethylene glycol), which has higher heat storage capacity per unit mass, higher average heat flux, and better unit synchronisation performance, so that it is more suitable for solar heating and cascade heat storage units in cold regions of Inner Mongolia. This study takes into account the irradiation variation of typical days during the winter heating season. The results show that the palmitic acid and polyethylene glycol combination scheme has the highest total heat storage per unit mass. This scheme also performs well in the synchronisation of two-stage storage units. When compared to the other two schemes, the average heat flux is increased by 25.5% for the first stage unit and 16.8% for the second stage unit.
Forest Management Communities’ Participation in Bioenergy Production Initiatives: A Case Study for Galicia (Spain)
Authors: Fernández-González Raquel , Guillén Félix Puime , Manta Otilia , Apostu Simona Andreea , Vasile Valentina
Published: October 2022

Bioenergy is the form of clean energy with the greatest potential for growth in Spain, especially in those regions with a large forest area and a high potential for the utilization of forest resources for energy purposes. This is the case in Galicia (a region located in northwestern Spain), where the communal management of forest resources is widespread. Within this type of management, there is a pioneering case study in which biomass energy use activities have been initiated through an international project. The possible success of this project is of great importance since it could represent an alternative to the main energy activity in the common management of forests: the production of wind energy. In recent years, and with a special increase in 2022, Galician public opinion has expressed its opposition to the implementation of new wind farms in its forests. The aim of this article is to analyze whether or not it is profitable for forest management communities to participate in bioenergy initiatives, which is a novel analysis of the Galician geographical area. For this purpose, the main economic and financial variables of the Galician forest management communities participating in the bioenergy project, SilvaPlus Project, have been analyzed, before and during their participation in the project. In addition, a financial comparison is also made with a group of Galician forest management communities without the energy use of their resources. The evolution of the relevant variables, analyzed between 2011 and 2020, shows a generally positive trend in the values of the forest communities participating in the bioenergy generation project, resulting in a positive experience, both environmentally and financially, for the forest community.
Applicability of Wake Models to Predictions of Turbine-Induced Velocity Deficit and Wind Farm Power Generation
Authors: Zhang Dongqin , Liang Yang , Li Chao , Xiao Yiqing , Hu Gang
Published: October 2022

Turbine-induced velocity deficit is the main reason to reduce wind farm power generation and increase the fatigue loadings. It is meaningful to investigate turbine-induced wake structures by a simple and accurate method. In this study, a series of single turbine wake models are proposed by combining different spanwise distributions and wake boundary expansion models. It is found that several combined wake models with high hit rates are more accurate and universal. Subsequently, the wake models for multiple wind turbines are also investigated by considering the combined wake models for single turbine and proper superposition approaches. Several excellent plans are provided where the velocity, turbulence intensity, and wind power generation for multiple wind turbines can be accurately evaluated. Finally, effects of thrust coefficient and ambient turbulence intensity are studied. In summary, the combined wake models for both single and multiple wind turbines are proposed and validated, enhancing the precision of wind farm layout optimization will be helped by using these wake models.
Modeling and Control of a Hybrid Multi DOF Motor for a Tilted Rotating System
Authors: Park Hyun-Jong , Go Sung-Chul
Published: October 2022

This paper presents the design, modeling, and control of a hybrid multi-degree-of-freedom motor (HMDOF motor) that can be applied to unmanned aerial vehicles, such as drones. The HMDOF motor has a rotating motor and tilting motors separately and enables multi-DOF movement by driving each motor. In addition, owing to its structural characteristics, it is designed to allow a 3- or 6-DOF movement in only one motor. In this study, the control performance of an HMDOF motor was verified using simulated and experimental results. The position control performance for the rotation speed of the rotating motor was verified, and the control performance of the motor under the speed of the rotating motor and disturbance on tilting motors were analyzed.