bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-Coding RNAs and Hereditary Hemorrhagic Telangiectasia
Authors: Cannavicci Anthony , Zhang Qiuwang , Kutryk Michael J. B.
Published: October 2020

Non-coding RNAs (ncRNAs) are functional ribonucleic acid (RNA) species that include microRNAs (miRs), a class of short non-coding RNAs (∼21–25 nucleotides), and long non-coding RNAs (lncRNAs) consisting of more than 200 nucleotides. They regulate gene expression post-transcriptionally and are involved in a wide range of pathophysiological processes. Hereditary hemorrhagic telangiectasia (HHT) is a rare disorder inherited in an autosomal dominant fashion characterized by vascular dysplasia. Patients can develop life-threatening vascular malformations and experience severe hemorrhaging. Effective pharmacological therapies are limited. The study of ncRNAs in HHT is an emerging field with great promise. This review will explore the current literature on the involvement of ncRNAs in HHT as diagnostic and pathogenic factors.
The Immune Microenvironment in Penile Cancer and Rationale for Immunotherapy
Authors: Ahmed Mohamed E. , Falasiri Shayan , Hajiran Ali , Chahoud Jad , Spiess Philippe E.
Published: October 2020

Penile cancer is an extremely rare malignancy that accounts for approximately 1% of cancer deaths in the United States every year. While primary penile cancer can be managed surgically, advanced and metastatic forms of the disease require more aggressive management plans with systemic chemotherapy and/or radiotherapy. Despite the meaningful response to systemic treatments, the 2-year progression-free survival and disease-specific survival have shown disappointing results. Therefore, there is a crucial need for alternative treatment options with more favorable outcomes and a lower toxicity profile. There are currently extensive studies of tumor molecular biology and clinical trials with targeted molecular therapies, such as PD-1, PD-L1, and CTLA-4. In this review, we will describe the penile cancer microenvironment, and summarize the rationale for immunotherapy in penile cancer patients.
Novel Orthodontic Cement Comprising Unique Imidazolium-Based Polymerizable Antibacterial Monomers
Authors: Lu Hui , Jin Xiaoming
Published: October 2020

White spot lesions (WSLs) can develop quickly and compromise the successful outcome of the orthodontic treatment. Orthodontic bonding cement with the capability to prevent or mitigate WSLs could be beneficial, especially for patients with high risk of caries. This study explored novel mono- and di-imidazolium-based polymerizable antibacterial monomers and evaluated orthodontic cement compositions comprising such novel monomers. Their antibacterial potentials, mechanical properties, and shear bond strength (SBS) to bovine enamel were investigated. Statistical tests were applied to SBS and mechanical tests (one-way ANOVA and Tukey’s test). For antibacterial resins C (ABR-C) and E (ABR-E), their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against cariogenic Streptococcus mutans bacterial strain UA159 were found to be 4 μg/mL and 8 μg /mL, respectively. The loss of dry mass from completely demineralized dentin beams in buffer solutions pre-dipped into ABR-C and ABR-E resins is much less than that in control buffer (artificial saliva) only. For unfilled resins comprising up to 12 wt % ABR-C, no significant decreases in flexural strength or modulus were observed. For experimental cements incorporating 1–4 wt % ABR-C, there was no drastic compromise to the SBS to enamel except for 3 wt % ABR-C. Furthermore, their SBS was all comparable to the commercially available orthodontic cements. The ISO-22196 antimicrobial test against S. aureus showed significant levels of antibacterial effects—up to over 5 logs of microorganism reduction exhibited by ABR-C-containing experimental cements. The imidazolium-based polymerizable monomers could be utilized to functionalize orthodontic bonding cement with steady antibacterial activity and develop a potential strategy to counteract WSLs.
An Analytical Model for Rock Cutting with a Chisel Pick of the Cutter Suction Dredger
Authors: Ouyang Yiping , Yang Qi , Chen Xinquan , Xu Yongfu
Published: October 2020

Cutter suction dredgers are important pieces of rock excavation equipment in port and waterway construction. It is valuable but difficult to properly estimate the cutting force on the chisel pick of the cutter suction dredger. In this paper, an analytical model, called the crushed zone expansion induced tensile failure model (CEIT model), is proposed for rock cutting with a chisel pick in order to predict the peak cutting force (Fc) more accurately. First, a review of the existing models for rock cutting with a chisel pick is presented. Next, based on the tensile breakage theory, cavity expansion theory and some hypotheses, the mathematical formula of the CEIT model is obtained. Different from that in the previous models, the effect of the rock on both sides of the chisel pick on Fc, defined as the sidewall effect is considered in the CEIT model. Then, the predicted Fc by the CEIT model is compared with the predicted Fc by existing theoretical models and experimental results to check the validity of the CEIT model. The results show that the CEIT model can well capture the relationships of Fc to the cutting parameters, including cutting width, cutting depth, and rake angle, and can predict the experimental results much better than the existing models. Finally, the sidewall effect and its influence factors according to the CEIT model are discussed.
Characterization of a Candida albicans Mutant Defective in All MAPKs Highlights the Major Role of Hog1 in the MAPK Signaling Network
Authors: Correia Inês , Wilson Duncan , Hube Bernhard , Pla Jesús
Published: October 2020

The success of Candida albicans as a pathogen relies on its ability to adapt and proliferate in different environmental niches. Pathways regulated by mitogen-activated protein kinases (MAPKs) are involved in sensing environmental conditions and developing an accurate adaptive response. Given the frequent cooperative roles of these routes in cellular functions, we have generated mutants defective in all combinations of the four described MAPKs in C. albicans and characterized its phenotype regarding sensitiveness to specific drugs, morphogenesis and interaction with host immune cells. We demonstrate that all MAPKs are dispensable in this yeast as a mutant defective in Cek1, Cek2, Mkc1 and Hog1 is viable although highly sensitive to oxidative and osmotic stress, displaying a specific pattern of sensitivity to antifungals. By comparing its phenotype with single, double and triple combinations of MAPK-deletion mutants we were able to unveil a Cek1-independent mechanism for Hog1 resistance to Congo red, and confirm the predominant effect of Hog1 on oxidative and osmotic adaptation. The quadruple mutant produces filaments under non-inducing conditions, but is unable to develop chlamydospores. Furthermore, cek1 cek2 mkc1 hog1 cells switch to the opaque state at high frequency, which is blocked by the ectopic expression of HOG1 suggesting a role of this kinase for phenotypic switching.
Using Expanded Natural Killer Cells as Therapy for Invasive Aspergillosis
Authors: Soe Win Mar , Lim Joan Hui Juan , Williams David L. , Goh Jessamine Geraldine , Tan Zhaohong , Sam Qi Hui , Chotirmall Sanjay H. , Ali Nur A’tikah Binte Mohamed , Lee Soo Chin , Seet Ju Ee , Ravikumar Sharada , Chai Louis Yi Ann
Published: October 2020

Invasive aspergillosis (IA) is a major opportunistic fungal infection in patients with haematological malignancies. Morbidity and mortality rates are high despite anti-fungal treatment, as the compromised status of immune system prevents the host from responding optimally to conventional therapy. This raises the consideration for immunotherapy as an adjunctive treatment. In this study, we evaluated the utility of expanded human NK cells as treatment against Aspergillus fumigatus infection in vitro and in vivo. The NK cells were expanded and activated by K562 cells genetically modified to express 4-1BB ligand and membrane-bound interleukin-15 (K562-41BBL-mbIL-15) as feeders. The efficacy of these cells was investigated in A. fumigatus killing assays in vitro and as adoptive cellular therapy in vivo. The expanded NK cells possessed potent killing activity at low effector-to-target ratio of 2:1. Fungicidal activity was morphotypal-dependent and most efficacious against A. fumigatus conidia. Fungicidal activity was mediated by dectin-1 receptors on the expanded NK cells leading to augmented release of perforin, resulting in enhanced direct cytolysis. In an immunocompromised mice pulmonary aspergillosis model, we showed that NK cell treatment significantly reduced fungal burden, hence demonstrating the translational potential of expanded NK cells as adjunctive therapy against IA in immunocompromised patients.
Local Disease-Free Survival Rate (LSR) Application to Personalize Radiation Therapy Treatments in Breast Cancer Models
Authors: Savoca Gaetano , Calvaruso Marco , Minafra Luigi , Bravatà Valentina , Cammarata Francesco Paolo , Iacoviello Giuseppina , Abbate Boris , Evangelista Giovanna , Spada Massimiliano , Forte Giusi Irma , Russo Giorgio
Published: October 2020

Cancer heterogeneity represents the main issue for defining an effective treatment in clinical practice, and the scientific community is progressively moving towards the development of more personalized therapeutic regimens. Radiotherapy (RT) remains a fundamental therapeutic treatment used for many neoplastic diseases, including breast cancer (BC), where high variability at the clinical and molecular level is known. The aim of this work is to apply the generalized linear quadratic (LQ) model to customize the radiant treatment plan for BC, by extracting some characteristic parameters of intrinsic radiosensitivity that are not generic, but may be exclusive for each cell type. We tested the validity of the generalized LQ model and analyzed the local disease-free survival rate (LSR) for breast RT treatment by using four BC cell cultures (both primary and immortalized), irradiated with clinical X-ray beams. BC cells were chosen on the basis of their receptor profiles, in order to simulate a differential response to RT between triple negative breast and luminal adenocarcinomas. The MCF10A breast epithelial cell line was utilized as a healthy control. We show that an RT plan setup based only on α and β values could be limiting and misleading. Indeed, two other parameters, the doubling time and the clonogens number, are important to finely predict the tumor response to treatment. Our findings could be tested at a preclinical level to confirm their application as a variant of the classical LQ model, to create a more personalized approach for RT planning.
Novel Air Pollution Measurement System Based on Ethereum Blockchain
Authors: Sofia Daniele , Lotrecchiano Nicoletta , Trucillo Paolo , Giuliano Aristide , Terrone Luigi
Published: October 2020

The need to protect sensitive data is growing, and environmental data are now considered sensitive. The application of last-generation procedures such as blockchains coupled with the implementation of new air quality monitoring technology allows the data protection and validation. In this work, the use of a blockchain applied to air pollution data is proposed. A blockchain procedure has been designed and tested. An Internet of Things (IoT)-based sensor network provides air quality data in terms of particulate matter of two different diameters, particulate matter (PM)10 and PM2.5, volatile organic compounds (VOC), and nitrogen dioxide (NO2) concentrations. The dataset also includes meteorological parameters and vehicular traffic information. This work foresees that the data, recovered from traditional Not Structured Query Language (NoSQL) database, and organized according to some specifications, are sent to the Ethereum blockchain daily automatically and with the possibility to choose the period of interest manually. There was also the development of a transaction management and recovery system aimed at retrieving data, formatting it according to the specifications and organizing it into files of various formats. The blockchain procedure has therefore been used to track data provided by air quality monitoring networks unequivocally.
Special Issue: Advances in Vehicular Networks
Authors: Masini Barbara M. , Silva Cristiano M. , Balador Ali
Published: October 2020

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Landscapes and Services in Peri-Urban Areas and Choice of Housing Location: An Application of Discrete Choice Experiments
Authors: Torquati Biancamaria , Giacchè Giulia , Tempesta Tiziano
Published: October 2020

The recent decades have witnessed a significant increase in the population in peri-urban areas which led to a progressive transformation of peri-urban landscapes, and the reduced ability of agriculture to provide ecosystem services. In order to understand the complex relationships established in peri-urban areas between reference urban centre, urban services (US) and ecosystem services (ES), with particular attention to the landscape, a Discrete Choice Experiment (DCE) was carried out in the transitional peri-urban areas of six municipalities located near the city of Perugia (Italy). The two main goals of this study are analysing the effect of the presence of US and ES on the demand for housing, and exploring the implications in terms of peri-urban land use policy. The results highlight that the availability of some ES can have a significant impact on choice of housing location.
Influence of Fraction Particle Size of Pure Straw and Blends of Straw with Calcium Carbonate or Cassava Starch on Pelletising Process and Pellet
Authors: Lisowski Aleksander , Matkowski Patryk , Mieszkalski Leszek , Mruk Remigiusz , Stasiak Mateusz , Piątek Michał , Świętochowski Adam , Dąbrowska Magdalena , Obstawski Paweł , Bakoń Tomasz , Karpio Krzysztof
Published: October 2020

The aim of this study was to investigate the pressure agglomeration process of wheat straw (WS) and the blends of WS with calcium carbonate (CC) or cassava straw (CS) with a ratio of 6% wt./wt. from seven separate fractions with sizes in the range of 0.21–2.81 mm. The agglomeration was performed at a moisture of 30% wb and a material temperature of 78 °C, with a dose of 0.1 g, in a die of diameter 8 mm and height 80 mm. The effects of the process were evaluated based on the compaction parameters and the pellets’ density, tensile strength, and water absorption. The incorporation of additives into the WS improved the pellet process and quality. Refined results were achieved after adding CC, as compared to those achieved after adding CS, and the preferred particle size was in the range of 1.00–1.94 mm. This was because, under the given conditions, the back pressure in the die chamber significantly increased, allowing the achievement of a single pellet density of 800 kg·m−3. The pellets were resistant to compressive loads and cracked only at tensile strength of 6 MPa and a specific compression work of 6.5 mJ·mm−2. The addition of CC to the WS improved the strength of the adhesive and the cohesive bonds between the particles. The water absorption for the uncrushed pellets was considerably less than that for crushed pellets, which results in the safer storage of uncrushed pellets and excellent moisture absorption of crushed pellets. The addition of CC to the WS offers benefits in the form of pellet strength with a high water absorption capability. Notably, a study of crushed pellet litter under broiler rearing conditions and an analysis of the operational costs of using WS additives are required for implementing this study.
Pyrometric-Based Melt Pool Monitoring Study of CuCr1Zr Processed Using L-PBF
Authors: Artzt Katia , Siggel Martin , Kleinert Jan , Riccius Joerg , Requena Guillermo , Haubrich Jan
Published: October 2020

The potential of in situ melt pool monitoring (MPM) for parameter development and furthering the process understanding in Laser Powder Bed Fusion (LPBF) of CuCr1Zr was investigated. Commercial MPM systems are currently being developed as a quality monitoring tool with the aim of detecting faulty parts already in the build process and, thus, reducing costs in LPBF. A detailed analysis of coupon specimens allowed two processing windows to be established for a suitably dense material at layer thicknesses of 30 µm and 50 µm, which were subsequently evaluated with two complex thermomechanical-fatigue (TMF) panels. Variations due to the location on the build platform were taken into account for the parameter development. Importantly, integrally averaged MPM intensities showed no direct correlation with total porosities, while the robustness of the melting process, impacted strongly by balling, affected the scattering of the MPM response and can thus be assessed. However, the MPM results, similar to material properties such as porosity, cannot be directly transferred from coupon specimens to components due to the influence of the local part geometry and heat transport on the build platform. Different MPM intensity ranges are obtained on cuboids and TMF panels despite similar LPBF parameters. Nonetheless, besides identifying LPBF parameter windows with a stable process, MPM allowed the successful detection of individual defects on the surface and in the bulk of the large demonstrators and appears to be a suitable tool for quality monitoring during fabrication and non-destructive evaluation of the LPBF process.
Use of GRP Pipe Waste Powder as a Filler Replacement in Hot-Mix Asphalt
Authors: Beycioğlu Ahmet , Kaya Orhan , Yıldırım Zeynel , Bağrıaçık Baki , Dobiszewska Magdalena , Morova Nihat , Çetin Suna
Published: October 2020

There is an increasing global trend to find sustainable, environmentally friendly and cost-effective materials as an alternative to limited natural raw materials. Similarly, the use of waste materials has been gaining popularity in the production of hot-mix asphalt (HMA). In this study, the sustainable use of glass-fiber-reinforced polyester (GRP) pipe waste powder (GRP-WP), gathered from the cutting and milling process of GRP pipe production, utilizing it in asphalt mixes as a filler, is evaluated based on lab testing to find out: (i) if it produces similar or better performance compared to the most conventionally available filler material (limestone) and, (ii) if so, what would be the optimum GRP-WP filler content to be used in asphalt mixes. For this reason, an experimental test matrix consisting of 45 samples with three different amounts of binder content (4%, 4.5% and 5.0%), and a 5% filler content with five different percentages of the GRP-WP content (0%, 25%, 50%, 75% and 100% replacement by weight of the filler), was prepared to figure out which sample would produce the similar Marshall stability and flow values compared to the control samples while also satisfying specification limits. It was found that the samples with 4.5% binder content, 3.75% GRP-WP and 1.25% limestone filler content produced the results both satisfying the specification requirements and providing an optimum mix design. It is believed that use of GRP-WP waste in HMA production would be a very useful way of recycling GRP-WP.
Concrete for Precast Blocks: Binary and Ternary Combination of Sewage Sludge Ash with Diverse Mineral Residue
Authors: Baeza-Brotons Francisco , Payá Jordi , Galao Oscar , Alberti Marcos , Garcés Pedro
Published: October 2020

This paper proposes binary and ternary combinations of sewage sludge ash (SSA) with fly ash (FA), marble dust (MD) and rice husk ash (RHA) as partial replacements of Portland cement in concretes with a similar dosage to that used in precast blocks, with very dry consistency. Several physical-mechanical tests were carried out on concrete specimens with curing ages of 28 and 90 days: density, water absorption, capillary water absorption, ultrasonic pulse velocity and compressive strength. The combinations of residues significantly improve the properties of the cementitious systems: 30% replacement of Portland cement provides strength values similar to the reference sample, showing the synergetic effects of the combination of the mineral additions. The significance of this research relies on the combined use of the mineral additions as well as the use of them for the precast block industry. The results show synergies among the additions and even that some of them showed relevant improvements when they are used in combination, performing better than when used individually.
Hot Gas Pressure Forming of Ti-55 High Temperature Titanium Alloy Tubular Component
Authors: Wang Kehuan , Shi Chenyu , Zhu Shiqiang , Wang Yongming , Shi Jintao , Liu Gang
Published: October 2020

In this paper, hot gas pressure forming (HGPF) of Ti-55 high temperature titanium alloy was studied. The hot deformation behavior was studied by uniaxial tensile tests at temperatures ranging from 750 to 900 °C with strain rates ranging from 0.001 to 0.05 s−1, and the microstructure evolution during tensile tests was characterized by electron backscatter diffraction. Finite element (FE) simulation of HGPF was carried out to study the effect of axial feeding on thickness distribution. Forming tests were performed to validate this process for Ti-55 alloy. Results show that when the temperature was higher than 750 °C, the elongation was large enough for HGPF of Ti-55 alloy. Dynamic recrystallization (DRX) occurred during the tensile deformation, which could refine the microstructure. The thickness uniformity of the formed part could be improved by increasing feeding length. The maximum thinning ratio decreased from 27.7% to 11.5% with the feeding length increasing from 0 to 20 mm. A qualified Ti-55 alloy component was successfully formed at 850 °C, the microstructure was slightly refined after forming, and the average post-form yield strength and peak strength were increased by 8.7% and 6.9%, respectively. Pre-heat treatment at 950 °C before HGPF could obtain Ti-55 alloy tubular component with bimodal microstructure and further improve the post-form strength.
Creep Testing of Thermoplastic Fiber-Reinforced Polymer Composite Tubular Coupons
Authors: Doan Hai Giang Minh , Mertiny Pierre
Published: October 2020

Thermoplastic fiber-reinforced polymer composites (TP-FRPC) are gaining popularity in industry owing to characteristics such as fast part fabrication, ductile material properties and high resistance to environmental degradation. However, TP-FRPC are prone to time-dependent deformation effects like creep under sustained loading, which can lead to significant dimensional changes and affect the safe operation of structures. Previous research in this context has focused, mainly, on testing of flat coupons. In this study, a creep testing method for TP-FRPC tubular coupons was developed. Specimens were fabricated using tape winding and subjected to well-defined loading conditions, i.e., pure hoop tensile and pure axial compressive stress. Strain gauges and digital image correlation were both employed for strain measurements and were found to be in good agreement. The evolution of strain rate, Poisson’s ratio and creep compliance were investigated. The prediction of experimental data by the Burgers model and the Findley’s power law model were explored. The research findings suggest that the developed experimental and analysis approach provides valuable information for the design of material systems and structures.
Monotonic and Fatigue Behavior of EBM Manufactured Ti-6Al-4V Solid Samples: Experimental, Analytical and Numerical Investigations
Authors: Radlof Wiebke , Benz Christopher , Heyer Horst , Sander Manuela
Published: October 2020

The present study aims to carry out an experimental, analytical and numerical investigation of the monotonic and fatigue performance of electron beam melted Ti-6Al-4V structures. Therefore, tensile tests, multiple step tests and strain-life tests were performed on machined EBM Ti-6Al-4V solid samples. An elastic-plastic material model in combination with a numerical damage model was examined according to the experimental tensile tests. Analytical models proposed by Ramberg and Osgood, as well as Coffin and Manson were obtained to describe the cyclic stress-strain curves and strain-life curves, respectively. The fracture surfaces of the tested samples and the influence of different build directions were analyzed. A prediction of the static and fatigue material properties is of particular importance, e.g., for the safe application of additively manufactured load-bearing implant structures. Based on the determined analytical and numerical models, the material and product behavior of complex electron beam melted structures under cyclic loading and fatigue life determination can be investigated in the early stages of the product development process.
Particle Swarm Optimization for Predicting the Development Effort of Software Projects
Authors: Alanis-Tamez Mariana Dayanara , López-Martín Cuauhtémoc , Villuendas-Rey Yenny
Published: October 2020

Software project planning includes as one of its main activities software development effort prediction (SDEP). Effort (measured in person-hours) is useful to budget and bidding the projects. It corresponds to one of the variables most predicted, actually, hundreds of studies on SDEP have been published. Therefore, we propose the application of the Particle Swarm Optimization (PSO) metaheuristic for optimizing the parameters of statistical regression equations (SRE) applied to SDEP. Our proposal incorporates two elements in PSO: the selection of the SDEP model, and the automatic adjustment of its parameters. The prediction accuracy of the SRE optimized through PSO (PSO-SRE) was compared to that of a SRE model. These models were trained and tested using eight data sets of new and enhancement software projects obtained from an international public repository of projects. Results based on statistically significance showed that the PSO-SRE was better than the SRE in six data sets at 99% of confidence, in one data set at 95%, and statistically equal than SRE in the remaining data set. We can conclude that the PSO can be used for optimizing SDEP equations taking into account the type of development, development platform, and programming language type of the projects.
Constrained Variational-Hemivariational Inequalities on Nonconvex Star-Shaped Sets
Authors: Migórski Stanisław , Fengzhen Long
Published: October 2020

In this paper, we study a class of constrained variational-hemivariational inequality problems with nonconvex sets which are star-shaped with respect to a certain ball in a reflexive Banach space. The inequality is a fully nonconvex counterpart of the variational-hemivariational inequality of elliptic type since it contains both, a convex potential and a locally Lipschitz one. Two new results on the existence of a solution are proved by a penalty method applied to a variational-hemivariational inequality penalized by the generalized directional derivative of the distance function of the constraint set. In the first existence theorem, the strong monotonicity of the governing operator and a relaxed monotonicity condition of the Clarke subgradient are assumed. In the second existence result, these two hypotheses are relaxed and a suitable hypothesis on the upper semicontinuity of the operator is adopted. In both results, the penalized problems are solved by using the Knaster, Kuratowski, and Mazurkiewicz (KKM) lemma. For a suffciently small penalty parameter, the solution to the penalized problem solves also the original one. Finally, we work out an example on the interior and boundary semipermeability problem that ilustrate the applicability of our results.
Flux-Reducing Tendency of Pd-Based Membranes Employed in Butane Dehydrogenation Processes
Authors: Peters Thijs A. , Stange Marit , Bredesen Rune
Published: October 2020

We report on the effect of butane and butylene on hydrogen permeation through thin state-of-the-art Pd–Ag alloy membranes. A wide range of operating conditions, such as temperature (200–450 °C) and H2/butylene (or butane) ratio (0.5–3), on the flux-reducing tendency were investigated. In addition, the behavior of membrane performance during prolonged exposure to butylene was evaluated. In the presence of butane, the flux-reducing tendency was found to be limited up to the maximum temperature investigated, 450 °C. Compared to butane, the flux-reducing tendency in the presence of butylene was severe. At 400 °C and 20% butylene, the flux decreases by ~85% after 3 h of exposure but depends on temperature and the H2/butylene ratio. In terms of operating temperature, an optimal performance was found at 250–300 °C with respect to obtaining the highest absolute hydrogen flux in the presence of butylene. At lower temperatures, the competitive adsorption of butylene over hydrogen accounts for a large initial flux penalty.
Graphene Oxide-Based Membranes for Water Purification Applications: Effect of Plasma Treatment on the Adhesion and Stability of the Synthesized Membranes
Authors: Alnoor Omer , Laoui Tahar , Ibrahim Ahmed , Kafiah Feras , Nadhreen Ghaith , Akhtar Sultan , Khan Zafarullah
Published: October 2020

The adhesion enhancement of graphene oxide (GO) and reduced graphene oxide (rGO) layer in the underlying polyethersulfone (PES) microfiltration membrane is a crucial step towards developing a high-performance membrane for water purification applications. In the present study, we modified the surface of a PES microfiltration membrane with plasma treatment (PT) carried out at different times (2, 10, and 20 min). We studied the effect of PT on the adhesion, stability, and performance of the synthesized GO/rGO-PES membranes. The membranes’ surface morphology and chemistry were characterized using atomic force microscopy, field emission scanning electron microscopy, and Fourier transform infrared spectroscopy. The membrane performance was evaluated by conducting a diffusion test for potassium chloride (KCl) ions through the synthesized membranes. The results revealed that the 2 min PT enhanced the adhesion and stability of the deposited GO/rGO layer when compared to the other plasma-treated membranes. This was associated with an increase in the KCl ion rejection from ~27% to 57%. Surface morphology analysis at a high magnification was performed for the synthesized membranes before and after the diffusion test. Although the membrane’s rejection was improved, the analysis revealed that the GO layers suffered from micro/nano cracks, which negatively affected the membrane’s overall performance. The use of the rGO layer, however, helped in minimizing the GO cracks and enhanced the KCl ion rejection to approximately 94%. Upon increasing the number of rGO deposition cycles from three to five, the performance of the developed rGO-PES membrane was further improved, as confirmed by the increase in its ion rejection to ~99%.
A Study on Cross-Shaped Structure of Invar Material Using Cold Wire Laser Fillet Welding (PART I: Feasibility Study for Weldability)
Authors: Kim Du-Song , Pyo Changmin , Kim Jaewoong , Kim Jisun , Lee Hee-Keun
Published: October 2020

With the need for eco-friendly energy increasing rapidly due to global environmental issues, there is a rapidly increasing demand for liquefied natural gas (LNG). LNG is liquefied at minus 163 degrees Celsius, and its volume decreases to 1/600, giving it a relatively higher storage and transport efficiency than gaseous natural gas (NG). The material for the tanks that store cryogenic LNG must be a material with high impact toughness at cryogenic temperatures. Invar, which contains 36% nickel and has a very low coefficient of thermal expansion, is used for the membranes and corner structures of LNG cargo holds. The cross-shaped Invar structure used in an LNG cargo hold is manufactured through manual tungsten inert gas (TIG) fillet welding, which causes welding distortion and weldability problems. This study is a feasibility study that aims to reduce welding distortion, increase weldability with welding speed, and reduce the steps in an existing process by half by replacing the existing manufacturing method with automatic fiber laser fillet welding. Laser welding using fiber laser parameters are controlled for 1.5 and 3.0 mm thick Invar materials and weldability is secured through cross-section observation. Then, the optimal welding conditions with top and back beads secured are derived through a trial and error method.
A New Nodal-Integration-Based Finite Element Method for the Numerical Simulation of Welding Processes
Authors: Jia Yabo , Bergheau Jean-Michel , Leblond Jean-Baptiste , Roux Jean-Christophe , Bouchaoui Raihane , Gallée Sebastien , Brosse Alexandre
Published: October 2020

This paper aims at introducing a new nodal-integration-based finite element method for the numerical calculation of residual stresses induced by welding processes. The main advantage of the proposed method is to be based on first-order tetrahedral meshes, thus greatly facilitating the meshing of complex geometries using currently available meshing tools. In addition, the formulation of the problem avoids any locking phenomena arising from the plastic incompressibility associated with von Mises plasticity and currently encountered with standard 4-node tetrahedral elements. The numerical results generated by the nodal approach are compared to those obtained with more classical simulations using finite elements based on mixed displacement–pressure formulations: 8-node Q1P0 hexahedra (linear displacement, constant pressure) and 4-node P1P1 tetrahedra (linear displacement, linear pressure). The comparisons evidence the efficiency of the nodal approach for the simulation of complex thermal–elastic–plastic problems.
Effect of Quasi-Hydrostatic Pressure on Deformation Mechanism in Ti-10Mo Alloy
Authors: Jiang Baozhen , Emura Satoshi , Tsuchiya Koichi
Published: October 2020

The deformation mechanisms of Ti-10Mo (wt.%) alloy subjected to different quasi-hydrostatic pressure values were investigated under constrained compression using stage of high-pressure torsion apparatus. Deformation products contain {332}<113> mechanical twinning, stress-induced α″ martensitic phase and stress-induced ω phase. A volume expansion accompanied stress-induced α″ martensitic phase transformation is 2.06%. By increasing the applied pressure from 2.5 GPa to 5 GPa, the dominant deformation mechanism underwent a transition from stress-induced α″ martensitic phase transformation to {332}<113> mechanical twinning.
NMR-Based Metabolomic Analysis and Microbial Composition of Soil Supporting Burkea africana Growth
Authors: Nemadodzi Lufuno Ethel , Vervoort Jacques , Prinsloo Gerhard
Published: October 2020

Burkea africana is a leguminous tree used for medicinal purposes, growing in clusters, on soils impoverished from most nutrients. The study aimed to determine the factors responsible for successful reproduction and establishment of the B. africana trees in nature, as all efforts for commercial production has been proven unsuccessful. An investigation was carried out to determine the metabolomic profile, chemical composition, and microbial composition of the soils where B. africana grows (Burkea soil) versus the soil where it does not grow (non-Burkea soil). 1H-NMR metabolomic analysis showed different metabolites in the respective soils. Trehalose and betaine, as well as a choline-like and carnitine-like compound, were found to be in higher concentration in Burkea soils, whereas, acetate, lactate, and formate were concentrated in non-Burkea soils. Liquid Chromatography-Mass Spectrometry analysis revealed the presence of numerous amino acids such as aspartic acid and glutamine to be higher in Burkea soils. Since it was previously suggested that the soil microbial diversity is the major driver for establishment and survival of seedlings in nature, Deoxyribonucleic acid (DNA) was extracted and a BLAST analysis conducted for species identification. Penicillium species was found to be highly prevalent and discriminant between the two soils, associated with the Burkea soils. No differences in the bacterial composition of Burkea and non-Burkea soils were observed. The variances in fungal composition suggests that species supremacy play a role in development of B. africana trees and is responsible for creating a supporting environment for natural establishment and survival of seedlings.
Metabolites from Microbes Isolated from the Skin of the Panamanian Rocket Frog Colostethus panamansis (Anura: Dendrobatidae)
Authors: Martin H. Christian , Ibáñez Roberto , Nothias Louis-Félix , Caraballo-Rodríguez Andrés Mauricio , Dorrestein Pieter C. , Gutiérrez Marcelino
Published: October 2020

The Panamanian rocket frog Colostethus panamansis (family Dendrobatidae) has been affected by chytridiomycosis, a deadly disease caused by the fungus Batrachochytrium dendrobatidis (Bd). While there are still uninfected frogs, we set out to isolate microbes from anatomically distinct regions in an effort to create a cultivable resource within Panama for potential drug/agricultural/ecological applications that perhaps could also be used as part of a strategy to protect frogs from infections. To understand if there are specific anatomies that should be explored in future applications of this resource, we mapped skin-associated bacteria of C. panamansis and their metabolite production potential by mass spectrometry on a 3D model. Our results indicate that five bacterial families (Enterobacteriaceae, Comamonadaceae, Aeromonadaceae, Staphylococcaceae and Pseudomonadaceae) dominate the cultivable microbes from the skin of C. panamansis. The combination of microbial classification and molecular analysis in relation to the anti-Bd inhibitory databases reveals the resource has future potential for amphibian conservation.
Feature-Based Molecular Networking to Target the Isolation of New Caffeic Acid Esters from Yacon (Smallanthus sonchifolius, Asteraceae)
Authors: Padilla-González Guillermo F. , Sadgrove Nicholas J. , Ccana-Ccapatinta Gari V. , Leuner Olga , Fernandez-Cusimamani Eloy
Published: October 2020

Smallanthus sonchifolius (yacon) is an edible tuberous Andean shrub that has been included in the diet of indigenous people since before recorded history. The nutraceutical and medicinal properties of yacon are widely recognized, especially for the improvement of hyperglycemic disorders. However, the chemical diversity of the main bioactive series of caffeic acid esters has not been explored in detail. In this metabolomics study, we applied the latest tools to facilitate the targeted isolation of new caffeic acid esters. Using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), we analyzed extracts from different organs (roots, vascular tissues of the stems, stem epidermis, leaves, bracts, and ray flowers) and followed a feature-based molecular networking approach to characterize the structural diversity of caffeic acid esters and recognize new compounds. The analysis identified three potentially new metabolites, one of them confirmed by isolation and full spectroscopic/spectrometric assignment using nuclear magnetic resonance (NMR), high-resolution mass spectrometry (HRMS), and MS/MS. This metabolite (5-O-caffeoyl-2,7-anhydro-d-glycero-β-d-galacto-oct-2-ulopyranosonic acid), along with eight known caffeic acid esters, was isolated from the roots and stems. Furthermore, based on detailed tandem MS analyses, we suggest that the two isomeric monocaffeoyl-2,7-anhydro-2-octulopyranosonic acids found in yacon can be reliably distinguished based on their characteristic MS2 and MS3 spectra. The outcome of the current study confirms the utility of feature-based molecular networking as a tool for targeted isolation of previously undescribed metabolites and reveals the full diversity of potentially bioactive metabolites from S. sonchifolius.
Leaves and Spiny Burs of Castanea Sativa from an Experimental Chestnut Grove: Metabolomic Analysis and Anti-Neuroinflammatory Activity
Authors: Chiocchio Ilaria , Prata Cecilia , Mandrone Manuela , Ricciardiello Fortuna , Marrazzo Pasquale , Tomasi Paola , Angeloni Cristina , Fiorentini Diana , Malaguti Marco , Poli Ferruccio , Hrelia Silvana
Published: October 2020

Castanea sativa cultivation has been present in Mediterranean regions since ancient times. In order to promote a circular economy, it is of great importance to valorize chestnut groves’ by-products. In this study, leaves and spiny burs from twenty-four Castanea trees were analyzed by 1H NMR metabolomics to provide an overview of their phytochemical profile. The Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) performed on these data allowed us to distinguish ‘Marrone’ from ‘Castagna’, since the latter were generally more enriched with secondary metabolites, in particular, flavonoids (astragalin, isorhamnetin glucoside, and myricitrin) were dominant. Knowing that microglia are involved in mediating the oxidative and inflammatory response of the central nervous system, the potential anti-inflammatory effects of extracts derived from leaves and spiny burs were evaluated in a neuroinflammatory cell model: BV-2 microglia cells. The tested extracts showed cytoprotective activity (at 0.1 and 0.5 mg/mL) after inflammation induction by 5 µg/mL lipopolysaccharide (LPS). In addition, the transcriptional levels of IL-1β, TNF-α, and NF-kB expression induced by LPS were significantly decreased by cell incubation with spiny burs and leaves extracts. Taken together, the obtained results are promising and represent an important step to encourage recycling and valorization of chestnut byproducts, usually considered “waste”.
Adipocytokines and Metabolic Syndrome in Patients with Schizophrenia
Authors: Mednova Irina A. , Boiko Anastasiia S. , Kornetova Elena G. , Parshukova Daria A. , Semke Arkadiy V. , Bokhan Nikolay A. , Loonen Anton J. M. , Ivanova Svetlana A.
Published: October 2020

The adipokines leptin, adiponectin, tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6) might be associated with metabolic syndrome (MetS) in patients with schizophrenia. In the present study, we attempted to confirm the results of previous reports and assessed their MetS-related correlation with body fat composition and biochemical parameters. We measured in 46 patients with schizophrenia and MetS serum levels of adiponectin insulin, leptin, TNF-α and IL-6 and compared these levels to those of patients with schizophrenia without MetS. The MetS patients had significantly increased leptin levels and leptin/adiponectin ratios, as well as decreased adiponectin levels. Leptin levels correlated with several metabolic parameters, both in patients with and without MetS, including body fat percentage, total fat fold, and body mass index (BMI). Patients without abnormal MetS components had lower levels of leptin and leptin/adiponectin ratios compared with patients who had one or two MetS components. Leptin/adiponectin ratios were higher in patients who had four rather than three MetS components. Multiple regression analysis revealed multiple associations for leptin but only one for adiponectin, TNF-α, and IL-6. Our results support an important pathophysiological role for leptin more than adiponectin in patients with schizophrenia with MetS.
Antioxidant and Antibacterial Capacities of Origanum vulgare L. Essential Oil from the Arid Andean Region of Chile and its Chemical Characterization by GC-MS
Authors: Simirgiotis Mario J. , Burton Daniel , Parra Felipe , López Jéssica , Muñoz Patricio , Escobar Hugo , Parra Claudio
Published: October 2020

This study aimed to characterize the in vitro antioxidant and antibacterial properties of oregano (Origanum vulgare) essential oil, as well as its chemical composition. To our best knowledge, there are few studies on oregano grown in the arid Andes region, but none on the metabolites produced and their bioactivity. This work identified fifty metabolites by Gas Chromatography–Mass Spectrometry (GC-MS)—monoterpene hydrocarbons, oxygenated monoterpenes, phenolic monoterpenes, sesquiterpene hydrocarbons, and oxygenated sesquiterpenes—present in the essential oil of oregano collected in the Atacama Desert. The main components of essential oregano oil were thymol (15.9%), Z-sabinene hydrate (13.4%), γ-terpinene (10.6%), p-cymene (8.6%), linalyl acetate (7.2%), sabinene (6.5%), and carvacrol methyl ether (5.6%). The antibacterial tests showed that the pathogenic bacteria Staphylococcus aureus and Salmonella enterica and the phytopathogenic bacteria Erwinia rhapontici and Xanthomonas campestris were the most susceptible to oregano oil, with the lowest concentrations of oil necessary to inhibit their bacterial growth. Moreover, oregano oil showed antibacterial activity against bacteria associated with food poisoning. In conclusion, O. vulgare from the arid Andean region possesses an important antibacterial activity with a high potential in the food industry and agriculture.
Identification and Elimination of the Clinically Relevant Multi-Resistant Environmental Bacteria Ralstonia insidiosa in Primary Cell Culture
Authors: Nurjadi Dennis , Boutin Sébastien , Schmidt Katja , Ahmels Melinda , Hasche Daniel
Published: October 2020

In times of spreading multidrug-resistant bacteria, species identification and decontamination of cell cultures can be challenging. Here, we describe a mobile cell culture contaminant with “black dot”-like microscopic appearance in newly established irreplaceable hybridoma cell lines and its identification. Using 16S rRNA gene sequencing, species-specific PCRs, whole genome sequencing (WGS), and MALDI-TOF mass spectrometry, the contaminant was identified as the ubiquitous environmental and clinically relevant Gram-negative bacterium Ralstonia insidiosa (R. insidiosa), a strong biofilm producer. Further characterizations by transmission electron microscopy (TEM) and biochemical API test were not conclusive. Whole genome sequencing of our R. insidiosa isolate revealed numerous drug-resistance determinants. Genome-wide comparison to other Ralstonia species could not unambiguously designate our isolate to R. insidiosa (<95% average nucleotide identity) suggesting a potential novel species or subspecies, closely related to R. insidiosa and R. pickettii. After determining the antibiotic susceptibility profile, the hybridoma cell culture was successfully decontaminated with ciprofloxacin without affecting antibody production.
Model Driven Interoperability for System Engineering
Authors: Zacharewicz Gregory , Daclin Nicolas , Doumeingts Guy , Haidar Hezam
Published: October 2020

To keep up to date, manufacturing enterprises need to use the latest results from the ICT sector, especially when collaborating with external partners in a supply chain and exchanging products and data. This has led to dealing with an increasing amount of heterogeneous information exchanged between partners including machines (physical means), humans and IT in the Supply Chain of ICT Systems (SC-ICTS). In this context, interoperability management is becoming more and more critical, but paradoxically, it is not yet fully efficiently anticipated, controlled and accompanied to recover from incompatibilities issues or failures. This paper intends to present how enterprise modeling, enterprise interoperability and model driven approaches can lead, together with system engineering architecture, to contribute to developing and improving the interoperability in the SC-ICTs. Model Driven System Engineering Architecture (MDSEA) is based on Enterprise Modeling using GRAI Model and its extensions. It gives enterprise internal developments guidelines, but originally, MDSEA is not the considering interoperability that is required between partners when setting a collaboration in the frame of SC-ICTS. As a result, the MDSEA, extended with interoperability concerns, led to the design of the MDISE (Model Driven Interoperability System Engineering) framework, which capitalizes on the research on enterprise interoperability. To finish, some proposals are made to extend the Model System Tool Box (MSTB) and the use of MDISE for Cyber Physical System (CPS) that are relevant components of SC-ICTS.