bluuebunny/crossref_metadata_2025 huggingface dataset:
Banking and Eurodollars in Italy in the 1950s
Authors: Balaban Ioan Achim
Published: March 2022

This article challenges current interpretations of the rise of the Eurodollar market. It argues that rather than being the exclusive innovation of British banks, the Eurodollar market had also Italian origins. Foreign-currency lending in Italy in the 1950s was characterized by competitive behavior. I explain the accumulation of Eurodollar deposits by Italian banks as resulting from the fact that nonresident foreign-currency deposits were not subject to reserve requirements. Furthermore, I discuss the attitudes of the Bank of Italy regarding the financing of foreign-currency credits with nonresident dollar deposits (Eurodollars) and compare the Eurocurrency liabilities of Italian banks vis-à-vis those of the City of London. The comparison facilitates an approximate estimation of the size of the Eurocurrency market in the late 1950s and, even more importantly, a recalibration of the view that the City of London was the dominant Eurodollar player from the outset.
The Influencer Republic: Monetizing Political Speech on Social Media
Authors: De Gregorio Giovanni , Goanta Catalina
Published: March 2022

This paper addresses the specific challenges arising from the monetization of political speech on social media, and propose a normative argument to extend consumer disclosures to political speech. Political speech enjoys the highest degree of protection by national constitutions as well as supranational and international charters. Unlike commercial speech which usuallyenjoy less constitutional protection, political speech is the foundation of constitutional democracies. The blurring line between political and commercial speech introduces a new layer of complexity in tackling hidden political advertising. Indeed, political speech is likely to attract commercial speech inside a broader scope of protection with the result that potential limitations of this kind of speech would be required to pass a very strict test through the balance with other constitutional safeguards or legitimate interests according to the criteria of necessity, legitimacy and proportionality. This could also question the scope of other regulation designed to govern commercial speech like advertising. To this end, the paper compares regulatory and judicial interpretations adopted in Europe and the United States, and is structured as follows. In the first part, we explore the content monetization business models (including influencer marketing) used on social media, and we identify three types of influencer ‘personas’ who are prone to engage in political speech. The second part looks into the constitutional differences between commercial and political speech across the Atlantic. The third part provides the normative argument at the intersection between consumer law and freedom of expression, and the fourth part concludes.
The spatial structure of electrostatically forced Faraday waves
Authors: Dehe S. , Hartmann M. , Bandopadhyay A. , Hardt S.
Published: March 2022

The instability of the interface between a dielectric and a conducting liquid, excited by a spatially homogeneous interface-normal time-periodic electric field, is studied based on experiments and theory. Special attention is paid to the spatial structure of the excited Faraday waves. The dominant modes of the instability are extracted using high-speed imaging in combination with an algorithm evaluating light refraction at the liquid–liquid interface. The influence of the liquid viscosities on the critical voltage corresponding to the onset of instability and on the dominant wavelength is studied. Overall, good agreement with theoretical predictions that are based on viscous fluids in an infinite domain is demonstrated. Depending on the relative influence of the domain boundary, the patterns exhibit either discrete modes corresponding to surface harmonics or boundary-independent patterns. The agreement between experiments and theory confirms that the electrostatically forced Faraday instability is sufficiently well understood, which may pave the way to control electrostatically driven instabilities. Last but not least, the analogies to classical Faraday instabilities may enable new approaches to study effects that have so far only been observed for mechanical forcing.
On transport tensor of dynamically unresolved oceanic mesoscale eddies
Authors: Ryzhov E.A. , Berloff P.
Published: March 2022

Parameterizing mesoscale eddies in ocean circulation models remains an open problem due to the ambiguity with separating the eddies from large-scale flow, so that their interplay is consistent with the resolving skill of the employed non-eddy-resolving model. One way to address the issue is by using recently formulated dynamically filtered eddies. These eddies are obtained as the field errors of fitting some given reference ocean circulation into the employed coarse-grid ocean model. The main strengths are (i) no explicit spatio-temporal filter is needed for separating the large-scale and eddy flow components, (ii) the eddies are dynamically translated into the error-correcting forcing that perfectly augments the coarse-grid model towards reproducing the reference circulation. We uncovered physical properties of the eddies by interpreting involved nonlinear eddy/large-scale interactions via the classical flux-gradient relation. We described the eddies in terms of their full, space–time dependent transport tensor, which was made unique by constraining it to be the same for the potential vorticity, momentum and buoyancy fluxes. Both diffusive and advective parts of the transport tensor were found to be significant. The diffusive tensor component is characterised by polar eigenvalues and is further decomposed into isotropic and filamentation components. The latter component completely dominates, therefore, it should be taken into account by eddy parameterizations, which is not yet the case. We also showed that spatial inhomogeneities of the transport tensor components are important. Comparing these properties with those obtained for more common, locally filtered eddies revealed that they are distinctly different.
Molecular kinetic modelling of nanoscale slip flow using a continuum approach
Authors: Shan Baochao , Wang Peng , Wang Runxi , Zhang Yonghao , Guo Zhaoli
Published: March 2022

One major challenge for a continuum model to describe nanoscale confined fluid flows is the lack of a boundary condition that can capture molecular-scale slip behaviours. In this work, we propose a molecular-kinetic boundary condition to model the fluid–surface and fluid–fluid molecular interactions using the Lennard–Jones type potentials, and add a mean-field force to the momentum equation. This new boundary condition is then applied to investigate the nanoscale Couette and Poiseuille flows using the generalised hydrodynamic model developed by Guo et al. (Phys. Fluids, volume 18, issue 6, 2006a, 067107). The accuracy of our model is validated by molecular dynamics simulations and other models for a broad range of parameters including density, shear rate, wettability and channel width. Our simulation results reveal some unexpected and unintuitive slip behaviours at the nanoscale, including the epitaxial layering structure of fluids and the slip length minimum. The slip length minimum, which is analogous to the Knudsen minimum, can be explained by competing fluid–solid and fluid–fluid molecular interactions as density varies. A new scaling law is proposed for the slip length to account for not only the competing effect between the fluid–solid and fluid–fluid molecular interactions, but also many other physical mechanisms including the competition between the fluid internal potential energy and kinetic energy, and the confinement effect. While the slip length is nearly constant at the low shear rates, it increases rapidly at the high shear rates due to friction reduction. These molecular-scale slip behaviours are caused by energy corrugations at the fluid–solid interface where strong fluid–solid and fluid–fluid molecular interactions interplay.
Explaining the gradient: Requirements for theories of visual awareness
Authors: Schmidt Thomas , Biafora Melanie
Published: March 2022

We propose that any theory of visual awareness must explain the gradient of different awareness measures over experimental conditions, especially when those measures form double dissociations among each other. Theories meeting this requirement must be specific to the measured facets of awareness, such as motion, contrast, or color. Integrated information theory (IIT) lacks such specificity because it is an underconstrained theory with unspecific predictions.
Does the present moment depend on the moments not lived?
Authors: Brette Romain
Published: March 2022

Integrated information theory postulates that a conscious experience depends on a repertoire of hypothetical experiences (the axiom of information). This makes consciousness depend on the context that constrains the set of possibilities and on the scenarios imagined by the external observer, and not only on the system itself.
What is exactly the problem with panpsychism?
Authors: Pennartz Cyriel M. A.
Published: March 2022

Merker et al.'s critique calls for a deeper analysis of panpsychism. In principle, the concept of integrated information can be applied to photodiodes and subatomic particles, but I suggest the main obstacle is the lack of any evidence to confirm the presence of consciousness. Also MRW's perspectivalist theory illustrates the difficulties in synthesizing a full-fledged theory of consciousness.
Searching in the wrong place: Might consciousness reside in the brainstem?
Authors: Devor Marshall , Koukoui Mary , Baron Mark
Published: March 2022

Doubtless, the conscious brain integrates masses of information. But declaring that consciousness simply “emerges” when enough has accumulated, doesn't really explain how first person experience is implemented by neurons. Moreover, empirical observations challenge integrated information theory's (IIT) reliance on thalamo–cortical interactions as the information integrator. More likely, the cortex streams processed information to a still-enigmatic consciousness generator, one perhaps located in the brainstem.
The integrated information theory of agency
Authors: Desmond Hugh , Huneman Philippe
Published: March 2022

We propose that measures of information integration can be more straightforwardly interpreted as measures of agency rather than of consciousness. This may be useful to the goals of consciousness research, given how agency and consciousness are “duals” in many (although not all) respects.
Escaping from the IIT Munchausen method: Re-establishing the scientific method in the study of consciousness
Authors: Verschure Paul
Published: March 2022

Integrated information theory (IIT) is an example of “ironic science” and obstructs the scientific study of consciousness. By confusing the ontological status of a method to quantify network complexity with that of a theory of consciousness, IIT has to square the circle and spirals toward its panpsychism conclusion. I analyze the consequences of this fallacy and suggest how the study of consciousness can be brought back into the realm of rational, empirical science.
The integrated information theory of consciousness: Unmasked and identified
Authors: Merker Bjorn , Williford Kenneth , Rudrauf David
Published: March 2022

In our response to a truly diverse set of commentaries, we first summarize the principal topical themes around which they cluster, then address two “outlier” positions (the problem of consciousness has been solved vs. is intractable). Next, we address ways in which commentaries by non-integrated information theory (IIT) authors engage with the specifics of our IIT critique, turning finally to the four commentaries by IIT authors.
Developing soybean seed germination: low ABA and high EXP1 gene expression promote embryonic axis growth whereas the seed coat delays radicle protrusion
Authors: Montechiarini Nidia H. , Morandi Eligio N. , Gosparini Carlos O.
Published: March 2022

Seed germination implies an expansion process restarting the growth of the embryonic axis (Ax) and which is completed by radicle emergence through the seed covering layers. In developing soybean seeds, abscisic acid in Ax (ABAa) mainly inhibits Ax growth. Additionally, the expression of the EXP1 gene at the elongation zone (EZ) was found to be involved in the promotion of mature soybean Ax growth, which increased during water incubation and which was repressed by exogenous ABA. This work aimed to evaluate (1) the ABAa and EXP1 levels at the EZ and (2) the role of the seed coat (SC) in developing soybean seed germination. Whole seeds (Se), embryos (Em) and Ax at 25–45 d after anthesis (DAA) germinated in vitro, and germination performance increased with DAA. ABAa decreased in planta from 25 DAA until its critical non-inhibitory threshold (ABAc) at around physiological maturity (45 DAA). At earlier ages, the ABAc was reached during the in vitro incubation. Concomitantly, EXP1 transcripts accumulated with age into the pool of long-lived mRNAs and were up-regulated during incubation. Additionally, isolated Ax germinated faster, took up more water and increased its water potential more rapidly during incubation than Ax in Se. Also, a lower osmotic gradient was required to germinate at 45 DAA, when ABAa was no longer inhibitory. Simultaneously, the pressure to protrude SC through the micropylar area increased from 25 to 45 DAA. These results support the role of ABAa and EXP1 in controlling Ax growth and the SC in delaying radicle protrusion.
Leveraging frequency agility of an MIMO antenna cluster with a transmitter IC
Authors: Saleem Ali Raza , Luomaniemi Rasmus , Lehtovuori Anu , Stadius Kari , Kosunen Marko , Viikari Ville , Ryynänen Jussi
Published: March 2022

This paper presents the prototype demonstration where an integrated transmitter circuit drives a mobile handset terminal antenna in order to provide frequency tunability and multiple input multiple output (MIMO) operation across the 0.5–4.5 GHz frequency range. The transmitter implementation incorporates on-chip weighted signal generation, i.e. amplitude and phase scaling to provide sufficient MIMO performance in the low band (700–960 MHz) and in the high band (1.5–4.5 GHz). In the transmitter, two antenna elements are used for MIMO operation in the low band and another two in the high band. The transmitter integrated circuit (IC) is fabricated in a 28 nm bulk CMOS technology with an active on-chip area of 0.2 mm$^2$. A custom antenna measurement procedure is proposed here in order to support and verify active antenna measurements with transmitter IC. A measurement procedure for the transmitter system comprising the transmitter IC and four antenna clusters is developed and discussed in comparison with traditional passive antenna measurements. The measurement results demonstrate that the transmitter IC driving the antenna clusters provides total antenna efficiency of $-6.5$ dB to $-1.5$ dB, and envelope correlation coefficient below 0.4 across the designated frequency bands. The results indicate that the implemented transmitter IC successfully tunes frequency response of the antenna clusters, and enhances the MIMO operation of such mobile antennas.
Factors Influencing the Molecular Compositions and Distributions of Atmospheric Nitrogen‐Containing Compounds
Authors: Jiang Hongxing , Li Jun , Tang Jiao , Zhao Shizhen , Chen Yinjun , Tian Chongguo , Zhang Xiangyun , Jiang Bin , Liao Yuhong , Zhang Gan
Published: March 2022

Atmospheric nitrogen‐containing organic compounds (NOCs) are critical components of global nitrogen deposition and light‐absorption species. The sources and compositions of NOCs are complex and remain largely unknown. Here, NOCs in 55 ambient aerosol samples collected in Guangzhou, South China, were analyzed via ultrahigh‐resolution Fourier transform ion cyclotron resonance mass spectrometry in negative‐ion and positive‐ion electrospray ionization (ESI) modes. The molecular compositions of NOCs measured via ESI– and ESI+ exhibited considerable differences. NOCs detected in the negative mode were mainly composed of highly oxygenated organic nitrates (O/N = 6), whereas NOCs detected in the positive mode were mainly composed of reduced nitrogen‐containing compounds (e.g., amides and amino acids). CHN compounds potentially corresponding to amines and alkaloids showed low abundance in the detection modes. Non‐metric multidimensional scaling and individual compound correlation analyses showed that the molecular compositions of NOCs were mainly affected by anthropogenic activities and meteorological parameters. For example, anthropogenic activities such as biomass burning and secondary nitrogen‐chemistry processes led to the accumulation of aromatic and highly oxygenated NOCs during winter. During summer, higher OH radical concentrations and temperatures will result in more prevalent or persistent reduced aliphatic NOCs, particularly lipid‐like amines. Some variables (e.g., relative humidity) have distinct effects on the variation of different types of NOCs. More research is needed to reveal the influencing mechanisms. This study clarifies the molecular compositions of NOCs and the mechanisms by which various factors influence the molecular variations. The findings can guide the assessment of NOCs evolution and deposition.
Reconstructing antibody dynamics to estimate the risk of influenza virus infection
Authors: Tsang Tim K. , Perera Ranawaka A. P. M. , Fang Vicky J. , Wong Jessica Y. , Shiu Eunice Y. , So Hau Chi , Ip Dennis K. M. , Malik Peiris J. S. , Leung Gabriel M. , Cowling Benjamin J. , Cauchemez Simon
Published: March 2022

For >70 years, a 4-fold or greater rise in antibody titer has been used to confirm influenza virus infections in paired sera, despite recognition that this heuristic can lack sensitivity. Here we analyze with a novel Bayesian model a large cohort of 2353 individuals followed for up to 5 years in Hong Kong to characterize influenza antibody dynamics and develop an algorithm to improve the identification of influenza virus infections. After infection, we estimate that hemagglutination-inhibiting (HAI) titers were boosted by 16-fold on average and subsequently decrease by 14% per year. In six epidemics, the infection risks for adults were 3%–19% while the infection risks for children were 1.6–4.4 times higher than that of younger adults. Every two-fold increase in pre-epidemic HAI titer was associated with 19%–58% protection against infection. Our inferential framework clarifies the contributions of age and pre-epidemic HAI titers to characterize individual infection risk.
Impact of yellow fever virus envelope protein on wild-type and vaccine epitopes and tissue tropism
Authors: Davis Emily H. , Wang Binbin , White Mellodee , Huang Yan-Jang S. , Sarathy Vanessa V. , Wang Tian , Bourne Nigel , Higgs Stephen , Barrett Alan D. T.
Published: March 2022

The envelope (E) protein of flaviviruses is functionally associated with viral tissue tropism and pathogenicity. For yellow fever virus (YFV), viscerotropic disease primarily involving the liver is pathognomonic for wild-type (WT) infection. In contrast, the live-attenuated vaccine (LAV) strain 17D does not cause viscerotropic disease and reversion to virulence is associated with neurotropic disease. The relationship between structure-function of the E protein for WT strain Asibi and its LAV derivative 17D strain is poorly understood; however, changes to WT and vaccine epitopes have been associated with changes in virulence. Here, a panel of Asibi and 17D infectious clone mutants were generated with single-site mutations at the one membrane residue and each of the eight E protein amino acid substitutions that distinguish the two strains. The mutants were characterized with respect to WT-specific and vaccine-specific monoclonal antibodies (mAbs) binding to virus plus binding of virus to brain, liver, and lung membrane receptor preparations (MRPs) generated from AG129 mice. This approach shows that amino acids in the YFV E protein domains (ED) I and II contain the WT E protein epitope, which overlap with those that mediate YFV binding to mouse liver. Furthermore, amino acids in EDIII associated with the vaccine epitope overlap with those that facilitate YFV binding mouse brain MRPs. Taken together, these data suggest that the YFV E protein is a key determinant in the phenotype of WT and 17D vaccine strains of YFV.
Big data collection in pharmaceutical manufacturing and its use for product quality predictions
Authors: Žagar Janja , Mihelič Jurij
Published: March 2022

Advances in data science and digitalization are transforming the world, and the pharmaceutical industry is no exception. Multiple sensor-equipped manufacturing processes and laboratory analysis are the main sources of primary data, which have been utilized for the presented dataset of 1005 actual production batches of selected medicine. This dataset includes incoming raw material quality results, compression process time series and final product quality results for the selected product. The data is highly valuable for it provides an insight into every 10 seconds of the process trajectory for 1005 actual production batches along with product quality collected over several years. It therefore offers an opportunity to develop advanced analysis models and procedures which would lead to the omission of current conventional and time consuming laboratory testing. Benefits for both the industry and patient are obvious: reducing product lead times and costs of manufacture.
An aquatic microrobot for microscale flow manipulation
Authors: Subendran Satishkumar , Wang Chun-Fang , Loganathan Dineshkumar , Lu Yueh-Hsun , Chen Chia-Yuan
Published: March 2022

Microrobots have been developed and extensively employed for performing the variety tasks with various applications. However, the intricate fabrication and actuation processes employed for microrobots further restrict their multitudinous applicability as well as the controllability in high accuracy. As an alternative, in this work an aquatic microrobot was developed using a distinctive concept of the building block technique where the microrobot was built based on the block to block design. An in-house electromagnetic system as well as the control algorithm were developed to achieve the precise real-time dynamics of the microrobot for extensive applications. In addition, pivotal control parameters of the microrobot including the actuating waveforms together with the operational parameters were verified and discussed in conjunction with the magnetic intensity simulation. A mixing task was performed with high efficiency based on the trajectory planning and rotation control of the microrobot to demonstrate its capability in flow manipulation which can be advantageous for microreactor applications down the load. Aside from it, a dissolution test was further conducted to provide an on-demand flow agitation function of the microrobot for the next level of lab chip applications. The presented work with detail dynamic analysis is envisaged to provide a new look of microrobot control and functions from the engineering perspective with profoundly potential applications.
Contrasting roles for G-quadruplexes in regulating human Bcl-2 and virus homologues KSHV KS-Bcl-2 and EBV BHRF1
Authors: Kumar Shivani , Ramamurthy Chitteti , Choudhary Divya , Sekar Aashika , Patra Anupam , Bhavesh Neel Sarovar , Vivekanandan Perumal
Published: March 2022

Herpesviruses are known to acquire several genes from their hosts during evolution. We found that a significant proportion of virus homologues encoded by HSV-1, HSV-2, EBV and KSHV and their human counterparts contain G-quadruplex motifs in their promoters. We sought to understand the role of G-quadruplexes in the regulatory regions of viral Bcl-2 homologues encoded by KSHV (KS-Bcl-2) and EBV (BHRF1). We demonstrate that the KSHV KS-Bcl-2 and the EBV BHRF1 promoter G-quadruplex motifs (KSHV-GQ and EBV-GQ) form stable intramolecular G-quadruplexes. Ligand-mediated stabilization of KS-Bcl-2 and BHRF1 promoter G-quadruplexes significantly increased the promoter activity resulting in enhanced transcription of these viral Bcl-2 homologues. Mutations disrupting KSHV-GQ and EBV-GQ inhibit promoter activity and render the KS-Bcl-2 and the BHRF1 promoters non-responsive to G-quadruplex ligand. In contrast, promoter G-quadruplexes of human bcl-2 gene inhibit promoter activity. Further, KS-Bcl-2 and BHRF1 promoter G-quadruplexes augment RTA (a virus-encoded transcription factor)-mediated increase in viral bcl-2 promoter activity. In sum, this work highlights how human herpesviruses have evolved to exploit promoter G-quadruplexes to regulate virus homologues to counter their cellular counterparts.
Loss of a globally unique kelp forest from Oman
Authors: Coleman M. A. , Reddy M. , Nimbs M. J. , Marshell A. , Al-Ghassani S. A. , Bolton J. J. , Jupp B. P. , De Clerck O. , Leliaert F. , Champion C. , Pearson G. A. , Serrão E. A. , Madeira P. , Wernberg T.
Published: March 2022

Kelp forests are declining in many regions globally with climatic perturbations causing shifts to alternate communities and significant ecological and economic loss. Range edge populations are often at most risk and are often only sustained through localised areas of upwelling or on deeper reefs. Here we document the loss of kelp forests (Ecklonia radiata) from the Sultanate of Oman, the only confirmed northern hemisphere population of this species. Contemporary surveys failed to find any kelp in its only known historical northern hemisphere location, Sadah on the Dhofar coast. Genetic analyses of historical herbarium specimens from Oman confirmed the species to be E. radiata and revealed the lost population contained a common CO1 haplotype found across South Africa, Australia and New Zealand suggesting it once established through rapid colonisation throughout its range. However, the Omani population also contained a haplotype that is found nowhere else in the extant southern hemisphere distribution of E. radiata. The loss of the Oman population could be due to significant increases in the Arabian Sea temperature over the past 40 years punctuated by suppression of coastal upwelling. Climate-mediated warming is threatening the persistence of temperate species and precipitating loss of unique genetic diversity at lower latitudes.
Lung lymphatic thrombosis and dysfunction caused by cigarette smoke exposure precedes emphysema in mice
Authors: Summers Barbara D. , Kim Kihwan , Clement Cristina C. , Khan Zohaib , Thangaswamy Sangeetha , McCright Jacob , Maisel Katharina , Zamora Sofia , Quintero Stephanie , Racanelli Alexandra C. , Redmond David , D’Armiento Jeanine , Yang Jisheng , Kuang Amy , Monticelli Laurel , Kahn Mark L. , Choi Augustine M. K. , Santambrogio Laura , Reed Hasina Outtz
Published: March 2022

The lymphatic vasculature is critical for lung function, but defects in lymphatic function in the pathogenesis of lung disease is understudied. In mice, lymphatic dysfunction alone is sufficient to cause lung injury that resembles human emphysema. Whether lymphatic function is disrupted in cigarette smoke (CS)-induced emphysema is unknown. In this study, we investigated the effect of CS on lung lymphatic function. Analysis of human lung tissue revealed significant lung lymphatic thrombosis in patients with emphysema compared to control smokers that increased with disease severity. In a mouse model, CS exposure led to lung lymphatic thrombosis, decreased lymphatic drainage, and impaired leukocyte trafficking that all preceded the development of emphysema. Proteomic analysis demonstrated an increased abundance of coagulation factors in the lymph draining from the lungs of CS-exposed mice compared to control mice. In addition, in vitro assays demonstrated a direct effect of CS on lymphatic endothelial cell integrity. These data show that CS exposure results in lung lymphatic dysfunction and a shift in thoracic lymph towards a prothrombic state. Furthermore, our data suggest that lymphatic dysfunction is due to effects of CS on the lymphatic vasculature that precede emphysema. These studies demonstrate a novel component of CS-induced lung injury that occurs early in the pathogenesis of emphysema.
The expanding network of mineral chemistry throughout earth history reveals global shifts in crustal chemistry during the Proterozoic
Authors: Moore Eli K. , Golden Josh J. , Morrison Shaunna M. , Hao Jihua , Spielman Stephanie J.
Published: March 2022

Earth surface redox conditions are intimately linked to the co-evolution of the geosphere and biosphere. Minerals provide a record of Earth’s evolving surface and interior chemistry in geologic time due to many different processes (e.g. tectonic, volcanic, sedimentary, oxidative, etc.). Here, we show how the bipartite network of minerals and their shared constituent elements expanded and evolved over geologic time. To further investigate network expansion over time, we derive and apply a novel metric (weighted mineral element electronegativity coefficient of variation; wMEECV) to quantify intra-mineral electronegativity variation with respect to redox. We find that element electronegativity and hard soft acid base (HSAB) properties are central factors in mineral redox chemistry under a wide range of conditions. Global shifts in mineral element electronegativity and HSAB associations represented by wMEECV changes at 1.8 and 0.6 billion years ago align with decreased continental elevation followed by the transition from the intermediate ocean and glaciation eras to post-glaciation, increased atmospheric oxygen in the Phanerozoic, and enhanced continental weathering. Consequently, network analysis of mineral element electronegativity and HSAB properties reveal that orogenic activity, evolving redox state of the mantle, planetary oxygenation, and climatic transitions directly impacted the evolving chemical complexity of Earth’s crust.
Comparative transcriptome analysis of melon (Cucumis melo L.) reveals candidate genes and pathways involved in powdery mildew resistance
Authors: Zhao Zengqiang , Dong Yongmei , Wang Jianyu , Zhang Guoli , Zhang Zhibin , Zhang Aiping , Wang Zhijun , Ma Panpan , Li Youzhong , Zhang Xiying , Ye Chunxiu , Xie Zongming
Published: March 2022

Powdery mildew is a major disease in melon, primarily caused by Podosphaera xanthii (Px). Some melon varieties were resistant to powdery mildew, while others were susceptible. However, the candidate genes associated with resistance and the mechanism of resistance/susceptibility to powdery mildew in melon remain unclear. In this study, disease-resistant melon cultivar TG-1 and disease-susceptible melon cultivar TG-5 were selected for comparative transcriptome analysis. The results suggested that the numbers of differentially expressed genes (DEGs) in TG-5 was always more than that in TG-1 at each of the four time points after Px infection, indicating that their responses to Px infection may be different and that the active response of TG-5 to Px infection may be earlier than that of TG-1. Transcription factors (TFs) analysis among the DEGs revealed that the bHLH, ERF, and MYB families in TG-1 may play a vital role in the interaction between melon and powdery mildew pathogens. GO enrichment analysis of these DEGs in TG-5 showed that the SBP, HSF, and ERF gene families may play important roles in the early stage of melon development after Px infection. Finally, we speculated on the regulatory pathways of melon powdery mildew and found PTI and ABA signaling genes may be associated with the response to Px infection in melon.
Proteasomal turnover of the RhoGAP tumor suppressor DLC1 is regulated by HECTD1 and USP7
Authors: Frey Yannick , Franz-Wachtel Mirita , Macek Boris , Olayioye Monilola A.
Published: March 2022

The Rho GTPase activating protein Deleted in Liver Cancer 1 (DLC1) is frequently downregulated through genetic and epigenetic mechanisms in various malignancies, leading to aberrant Rho GTPase signaling and thus facilitating cancer progression. Here we show that in breast cancer cells, dysregulation of DLC1 expression occurs at the protein level through rapid degradation via the ubiquitin–proteasome system. Using mass spectrometry, we identify two novel DLC1 interaction partners, the ubiquitin-ligase HECTD1 and the deubiquitinating enzyme ubiquitin-specific-processing protease 7 (USP7). While DLC1 protein expression was rapidly downregulated upon pharmacological inhibition of USP7, siRNA-mediated knockdown of HECTD1 increased DLC1 protein levels and impaired its degradation. Immunofluorescence microscopy analyses revealed that the modulation of HECTD1 levels and USP7 activity altered DLC1 abundance at focal adhesions, its primary site of action. Thus, we propose opposing regulatory mechanisms of DLC1 protein homeostasis by USP7 and HECTD1, which could open up strategies to counteract downregulation and restore DLC1 expression in cancer.
Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E-derived arginine-rich amphipathic α-helical peptides
Authors: Takechi-Haraya Yuki , Ohgita Takashi , Kotani Mana , Kono Hiroki , Saito Chihiro , Tamagaki-Asahina Hiroko , Nishitsuji Kazuchika , Uchimura Kenji , Sato Takeshi , Kawano Ryuji , Sakai-Kato Kumiko , Izutsu Ken-ichi , Saito Hiroyuki
Published: March 2022

We previously developed an amphipathic arginine-rich peptide, A2-17, which has high ability to directly penetrate across cell membranes. To understand the mechanism of the efficient cell-penetrating ability of the A2-17 peptide, we designed three structural isomers of A2-17 having different values of the hydrophobic moment and compared their membrane interaction and direct cell penetration. Confocal fluorescence microscopy revealed that cell penetration efficiency of peptides tends to increase with their hydrophobic moment, in which A2-17 L14R/R15L, an A2-17 isomer with the highest hydrophobic moment, predominantly remains on plasma cell membranes. Consistently, Trp fluorescence analysis indicated the deepest insertion of A2-17 L14R/R15L into lipid membranes among all A2-17 isomers. Electrophysiological analysis showed that the duration and charge flux of peptide-induced pores in lipid membranes were prominent for A2-17 L14R/R15L, indicating the formation of stable membrane pores. Indeed, the A2-17 L14R/R15L peptide exhibited the strongest membrane damage to CHO-K1 cells. Atomic force microscopy quantitatively defined the peptide-induced membrane perturbation as the decrease in the stiffness of lipid vesicles, which was correlated with the hydrophobic moment of all A2-17 isomers. These results indicate that optimal membrane perturbation by amphipathic A2-17 peptide is critical for its efficient penetration into cells without inducing stabilized membrane pores.
The fish ability to accelerate and suddenly turn in fast maneuvers
Authors: Paniccia Damiano , Graziani Giorgio , Lugni Claudio , Piva Renzo
Published: March 2022

Velocity burst and quick turning are performed by fish during fast maneuvers which might be essential to their survival along pray–predator encounters. The parameters to evaluate these truly unsteady motions are totally different from the ones for cruising gaits since a very large acceleration, up to several times the gravity, and an extreme turning capability, in less than one body length, are now the primary requests. Such impressive performances, still poorly understood, are not common to other living beings and are clearly related to the interaction with the aquatic environment. Hence, we focus our attention on the water set in motion by the body, giving rise to the relevant added mass and the associated phenomena in transient conditions, which may unveil the secret of the great maneuverability observed in nature. Many previous studies were almost exclusively concentrated on the vortical wake, whose account, certainly dominant at steady state, is not sufficient to explain the entangled transient phenomena. A simple two-dimensional impulse model with concentrated vorticity is used for the self-propulsion of a deformable body in an unbounded fluid domain, to single out the potential and the vortical impulses and to highlight their interplay induced by recoil motions.
Alginate oligosaccharides enhance diffusion and activity of colistin in a mucin-rich environment
Authors: Stokniene Joana , Varache Mathieu , Rye Philip D. , Hill Katja E. , Thomas David W. , Ferguson Elaine L.
Published: March 2022

In a number of chronic respiratory diseases e.g. cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD), the production of viscous mucin reduces pulmonary function and represents an effective barrier to diffusion of inhaled therapies e.g. antibiotics. Here, a 2-compartment Transwell model was developed to study impaired diffusion of the antibiotic colistin across an artificial sputum (AS) matrix/medium and to quantify its antimicrobial activity against Pseudomonas aeruginosa NH57388A biofilms (alone and in combination with mucolytic therapy). High-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) revealed that the presence of AS medium significantly reduced the rate of colistin diffusion (> 85% at 48 h; p < 0.05). Addition of alginate oligosaccharide (OligoG CF-5/20) significantly improved colistin diffusion by 3.7 times through mucin-rich AS medium (at 48 h; p < 0.05). Increased diffusion of colistin with OligoG CF-5/20 was shown (using confocal laser scanning microscopy and COMSTAT image analysis) to be associated with significantly increased bacterial killing (p < 0.05). These data support the use of this model to study drug and small molecule delivery across clinically-relevant diffusion barriers. The findings indicate the significant loss of colistin and reduced effectiveness that occurs with mucin binding, and support the use of mucolytics to improve antimicrobial efficacy and lower antibiotic exposure.
Relaxation time approximations in PAOFLOW 2.0
Authors: Jayaraj Anooja , Siloi Ilaria , Fornari Marco , Nardelli Marco Buongiorno
Published: March 2022

Regardless of its success, the constant relaxation time approximation has limited validity. Temperature and energy dependent effects are important to match experimental trends even in simple situations. We present the implementation of relaxation time approximation models in the calculation of Boltzmann transport in 2.0 and apply those to model band-structures. In addition, using a self-consistent fitting of the model parameters to experimental conductivity data, we provide a flexible tool to extract scattering rates with high accuracy. We illustrate the approximations using simple models and then apply the method to GaAs, Si,$$\hbox {Mg}_3\hbox {Sb}_2$$Mg3Sb2, and$$\hbox {CoSb}_3$$CoSb3.
Production of precision slots in copper foil using micro EDM
Authors: Mouralova Katerina , Bednar Josef , Benes Libor , Plichta Tomas , Prokes Tomas , Fries Jiri
Published: March 2022

Electrical discharge machining (EDM) is an unconventional machining technology. It allows machining of at least at least electrically conductive materials. The trend of miniaturization of industrial products is obvious. However, the required quality and accuracy must be maintained, which can be achieved with micro-EDM. One of the industrial products is also optical devices used for testing cars. These contain miniaturized parts, which are, however, necessary for their proper functioning. For this reason, this study was performed, which focused on the production of a precise slot measuring 5000 × 170 µm in a copper foil with a thickness of 125 µm. The same copper foil was used as a tool, which represents an advance in the production of micro-parts.The use of the same semi-product for the production of the slit as well as the tool itself has not yet been presented in any similar study. A design of experiment Box and Behnken Response Surface Design was performed for a total of 15 rounds, monitoring the effect of machine setting parameters (Pulse current, Pulse on time and Voltage) on responses in the form of Erosion rate, corner radius, slot length and width. Using multi-criteria optimization, the optimal setting of the machine parameters for the production of a given slit was determined, which is Pulse current = 2.1 A, Pulse on time = 40 µs and Voltage = 238.8 V. Micro-EDM technology has been found to be suitable for the production of miniaturized slits.
Mitigating spread of contamination in meat supply chain management using deep learning
Authors: Amani Mohammad Amin , Sarkodie Samuel Asumadu
Published: March 2022

Industry 4.0 recommends a paradigm shift from traditional manufacturing to automated industrial practices, especially in different parts of supply chain management. Besides, the Sustainable Development Goal (SDG) 12 underscores the urgency of ensuring a sustainable supply chain with novel technologies including Artificial Intelligence to decrease food loss, which has the potential of mitigating food waste. These new technologies can increase productivity, especially in perishable products of the supply chain by reducing expenses, increasing the accuracy of operations, accelerating processes, and decreasing the carbon footprint of food. Artificial intelligence techniques such as deep learning can be utilized in various sections of meat supply chain management––where highly perishable products like spoiled meat need to be separated from wholesome ones to prevent cross-contamination with food-borne pathogens. Therefore, to automate this process and prevent meat spoilage and/or improve meat shelf life which is crucial to consumer meat preferences and sustainable consumption, a classification model was trained by the DCNN and PSO algorithms with 100% accuracy, which discerns wholesome meat from spoiled ones.
Sensitivity to orientation is not unique to social attention cueing
Authors: Vestner Tim , Gray Katie L. H. , Cook Richard
Published: March 2022

It is well-established that faces and bodies cue observers’ visuospatial attention; for example, target items are found faster when their location is cued by the directionality of a task-irrelevant face or body. Previous results suggest that these cueing effects are greatly reduced when the orientation of the task-irrelevant stimulus is inverted. It remains unclear, however, whether sensitivity to orientation is a unique hallmark of “social” attention cueing or a more general phenomenon. In the present study, we sought to determine whether the cueing effects produced by common objects (power drills, desk lamps, desk fans, cameras, bicycles, and cars) are also attenuated by inversion. When cueing stimuli were shown upright, all six object classes produced highly significant cueing effects. When shown upside-down, however, the results were mixed. Some of the cueing effects (e.g., those induced by bicycles and cameras) behaved liked faces and bodies: they were greatly reduced by orientation inversion. However, other cueing effects (e.g., those induced by cars and power drills) were insensitive to orientation: upright and inverted exemplars produced significant cueing effects of comparable strength. We speculate that (i) cueing effects depend on the rapid identification of stimulus directionality, and (ii) some cueing effects are sensitive to orientation because upright exemplars of those categories afford faster processing of directionality, than inverted exemplars. Contrary to the view that attenuation-by-inversion is a unique hallmark of social attention, our findings indicate that some non-social cueing effects also exhibit sensitivity to orientation.
The vibration study of a sandwich conical shell with a saturated FGP core
Authors: Nasr Esfahani Mohsen , Hashemian Mohammad , Aghadavoudi Farshid
Published: March 2022

This paper is provided to analyze the free vibration of a sandwich truncated conical shell with a saturated functionally graded porous (FGP) core and two same homogenous isotropic face sheets. The mechanical behavior of the saturated FGP is assumed based on Biot’s theory, the shell is modeled via the first-order shear deformation theory (FSDT), and the governing equations and boundary conditions are derived utilizing Hamilton’s principle. Three different porosity distribution patterns are studied including one homogenous uniform distribution pattern and two non-homogenous symmetric ones. The porosity parameters in mentioned distribution patterns are regulated to make them the same in the shell’s mass. The equations of motion are solved exactly in the circumferential direction via proper sinusoidal and cosinusoidal functions, and a numerical solution is provided in the meridional direction utilizing the differential quadrature method (DQM). The precision of the model is approved and the influences of several parameters such as circumferential wave number, the thickness of the FGP core, porosity parameter, porosity distribution pattern, the compressibility of the pore fluid, and boundary conditions on the shell’s natural frequencies are investigated. It is shown that the highest natural frequencies usually can be achieved when the larger pores are located close to the shell’s middle surface and in each vibrational mode, there is a special value of the porosity parameter which leads to the lowest natural frequencies. It is deduced that in most cases, natural frequencies decrease by increasing the thickness of the FGP core. In addition, reducing the compressibility of the porefluid a small growth in the natural frequencies can be seen.
A virtual laboratory based on full-field crystal plasticity simulation to characterize the multiscale mechanical properties of AHSS
Authors: Ma Hongyue , Li Yangqi , Zhang Haiming , Li Qian , Chen Fei , Cui Zhenshan
Published: March 2022

In this work, we proposed a virtual laboratory based on full-field crystal plasticity (CP) simulation to track plastic anisotropy and to calibrate yield functions for multiphase metals. The virtual laboratory, minimally, only requires easily accessible EBSD data for constructing the highly-resolved microstructural representative volume element and macroscopic flow stress data for identifying the micromechanical parameters of constituent phases. An inverse simulation method based on a global optimization scheme was developed to identify the CP parameters, and a nonlinear least-squares method was employed to calibrate yield functions. Mechanical tests of advanced high strength steel sheet under various loading conditions were conducted to validate the virtual laboratory. Three well-known yield functions, the quadratic Hill48 and non-quadratic Yld91 and Yld2004-18p yield functions, were selected as the validation benchmarks. All the studied functions, calibrated by numerous stress points of arbitrary loading conditions, successfully captured both the deformation and strength anisotropies. The full-field CP modeling correlated well the microscopic deformation mechanism and plastic heterogeneity with the macromechanical behavior of the sheet. The proposed virtual laboratory, which is readily extended with physically based CP models, could be a versatile tool to explore and predict the mechanical property and plastic anisotropy of advanced multiphase materials.