bluuebunny/crossref_metadata_2025 huggingface dataset:
Ontologies for cloud robotics
Authors: Pignaton de Freitas Edison , Bermejo-Alonso Julita , Khamis Alaa , Li Howard , Olszewska Joanna Isabelle
Published: June 2020

Cloud robotics (CR) is currently a growing area in the robotic community. Indeed, the use of cloud computing to share data and resources of distributed robotic systems leads to the design and development of cloud robotic systems (CRS) which constitute useful technologies for a wide range of applications such as smart manufacturing, aid and rescue missions. However, in order to get coherent agent-to-cloud communications and efficient agent-to-agent collaboration within these CRS, there is a need to formalize the knowledge representation in CR. Hence, the use of ontologies provides a mean to define formal concepts and their relations in an interoperable way. This paper presents standard robotic ontologies and their extension in the CR domain as well as their possible implementations in the case of a real-world CR scenario.
Speed, law and the global economy: How economic acceleration contributes to inequality and precarity
Authors: Perrone Nicolás M.
Published: June 2020

The law is implicated in many of the relations that produce inequality and precarity in the global economy. It contributes in different ways to the unequal bargaining power between states, capital, and labour. One way that has attracted less attention so far relates to how the law accelerates economic relations. This article examines the role of law in the global economy not by focusing on the international economic institutions, such as the World Trade Organization (WTO), the European Union (EU) or the International Monetary Fund (IMF), but on the transactions that plug maquila workers and freelancers into the global economy. The argument is that the speed of these economic relations favours those who command international production, creating what Hartmut Rosa calls a ‘frenetic standstill’. Importantly, the law can also contribute to changing these bargaining dynamics by slowing down some of these transactions and facilitating their territorial re-embedding. This strategy, however, requires a better understanding of the role of law in transnational networks of contracts as well as more state and non-state international co-ordination: The opposite of nationalist attitudes, such as Brexit and Trump’s trade policy.
Air temperature lapse rates and cloud cover in a hyper-oceanic climate
Authors: Fitzgerald Nicholas B. , Kirkpatrick Jamie B.
Published: June 2020

Air temperature lapse rates vary geographically and temporally. Sub-Antarctic Macquarie Island provides an opportunity to compare lapse rates between windward and leeward slopes in a hyper-oceanic climate. Lapse rates were steep by global standards, typically close to the dry adiabatic lapse rate despite the near-constant high humidity. Limited diurnal and seasonal variation occurs in lapse rates on Macquarie Island. High variability of lapse rates on the eastern (lee) slope in summer months and in the midday hours appears to be driven by solar radiation. No diurnal or seasonal pattern was evident on the western slope. Development of orographic cloud is expected to modify lapse rates, given the theoretical shift between dry and saturated adiabatic lapse rates that occurs with condensation of water vapour. Cloud cover was frequent, with higher elevations being under cloud 50% of the time, with no seasonal variation. However, cloud base level did not explain variation in lapse rates. Low cloud is likely to be of ecological importance because it influences fog precipitation, solar radiation and evapotranspiration. Year-round dominance of westerly airflows and limited seasonal variation in air temperature and humidity explain the limited seasonal variation in cloud cover and lapse rates on Macquarie Island.
Understanding Violence
Authors: Vincent Mary
Published: June 2020

Any civil war leaves a legacy of partisanship. Divisions persist over time and may be particularly bitter when, as in Spain, a culture of victory survives long after the end of hostilities. Any attempt at reconciliation was postponed, leading to an unusually bifurcated historiography, framed by a perennial interest into who, at base, was responsible for the outbreak of the civil war. The parameters of this debate were set in the 1970s, most notably in works by Stanley Payne and Paul Preston. It has continued in various guises since then, most recently revived by a generation of Spanish scholars, such as Fernando del Rey Reguillo, who have added case studies and new levels of detail, while leaving the terms of the debate more or less unchanged. Of course the historiographical panorama can change, often in tandem with the historical context, as several contributions to this roundtable make clear, notably those of Vjeran Pavlaković, Helen Graham and Giuliana Chamedes. However, the framing of the Spanish Civil War is still essentially moral: who bore responsibility for the outbreak of war, who was to blame for the defeat of the republic and, as a consequence, the conduct of the repression. One result has been to assimilate the history of the civil war with that of the Second Republic; another is a historiography that is largely political in tone and focus.
How to Legalize Medically Assisted Death in a Free and Democratic Society
Authors: BROWNE ALISTER , RUSSELL J.S.
Published: June 2020

In 2015, the Supreme Court of Canada struck down the criminal law prohibiting physician assisted death in Canada. In 2016, Parliament passed legislation to allow what it called ‘medical assistance in dying (MAID).’ The authors first describe the arguments the Court used to strike down the law, and then argue that MAID as legalized in Bill C-14 is based on principles that are incompatible with a free and democratic society, prohibits assistance in dying that should be permitted, and makes access to medically-assisted death unnecessarily difficult. They then propose a version of MAID legislation (‘Ideal MAID’) that gives proponents and opponents of MAID everything they can legitimately want, contend that it is the only way to legalize MAID that is compatible with a free and democratic society, and conclude that it is the way to legalize MAID in Canada and other similarly free and democratic societies.
Counting Cases of Termination of Life without Request: New Dances with Data
Authors: DEN HARTOGH GOVERT
Published: June 2020

This paper explores the common argument proposed by opponents of the legalization of euthanasia that permitting ending a patient’s life at their request will lead to the eventual legalization of terminating life without request. The author’s examination of data does not support the conclusion that a causal connection exists between legalizing ending of life on request and an increase in the number of cases without request.
Incorporation of arginine, glutamine or leucine in culture medium accelerates in vitro activation of primordial follicles in 1-day-old mouse ovary
Authors: Alborzi Parimah , Jafari Atrabi Mohammad , Akbarinejad Vahid , Khanbabaei Ramezan , Fathi Rouhollah
Published: June 2020

In vitro activation of primordial follicles provides cancer patients subjected to oncotherapy with a safe therapeutic strategy for fertility preservation, however a successful protocol for activation of primordial follicles in prepubertal patients has not yet been defined comprehensively. There is evidence that amino acids such as leucine, arginine and glutamine could stimulate the mammalian target of rapamycin (mTOR) pathway, which plays a pivotal role in primordial follicle activation. Nevertheless, there has been no report that elucidates the effect of these amino acids on in vitro development of ovarian follicles. Therefore, the present study was conducted to evaluate the effects of these amino acids and their combination on the formation and activation of primordial follicles in 1-day-old murine ovaries during an 11-day culture period. The experimental groups consisted of base medium (BM), base medium + arginine (ARG), base medium + glutamine (GLU), base medium + leucine (LEU) and base medium + a combination of arginine, glutamine and leucine (AGL). The proportions of different stages of ovarian follicles and gene expression of regulatory factors were assessed using histology and quantitative real-time PCR on days 5 and 11 of culture. The proportion of transitional and primary follicles was greater in all amino acid-treated groups compared with the BM group (P < 0.05). Moreover, leucine resulted in elevated expression of Gdf9 and Bmp15, and glutamine augmented the expression of Pi3k on day 11 of culture. In conclusion, the present study showed that inclusion of leucine, glutamine, arginine or their combination in the culture medium for murine ovarian tissue could accelerate the activation of primordial follicles and alter the expression of the corresponding factors.
Six-Hour Manual Ventilation with a Bag-Valve-Tube Device by Briefly Trained Non-Medical Personnel is Feasible
Authors: Maklada Nana , Katz Shalhav Malka , Lagazzi Emanuele , Halpern Pinchas
Published: June 2020

Rationale:

Manual ventilation with a bag-valve device (BVD) is a Basic Life Support skill. Prolonged manual ventilation may be required in resource-poor locations and in severe disasters such as hurricanes, pandemics, and chemical events. In such circumstances, trained operators may not be available and lay persons may need to be quickly trained to do the job.

Objectives:

The current study investigated whether minimally trained operators were able to manually ventilate a simulated endotracheally intubated patient for six hours.

Methods:

Two groups of 10 volunteers, previously unfamiliar with manual ventilation, received brief, structured BVD-tube ventilation training and performed six hours of manual ventilation on an electronic lung simulator. Operator cardiorespiratory variables and perceived effort, as well as the quality of the delivered ventilation, were recorded. Group One ventilated a “normal lung” (compliance 50cmH2O/L, resistance 5cmH2O/L/min). Group Two ventilated a “moderately injured lung” (compliance 20cmH2O/L, resistance 20cmH2O/L/min).

Results:

Volunteers’ blood pressure, heart rate (HR), respiratory rate (RR), and peripheral capillary oxygen saturation (SpO2) were stable throughout the study. Perceived effort was minimal. The two groups provided clinically adequate and similar RRs (13.3 [SD = 3.0] and 14.1 [SD = 2.5] breaths/minute, respectively) and minute volume (MV; 7.6 [SD = 2.1] and 7.7 [SD = 1.4] L/minute, respectively).

Conclusions:

The results indicate that minimally trained persons can effectively perform six hours of manual BVD-tube ventilation of normal and moderately injured lungs, without undue effort. Quality of delivered ventilation was clinically adequate.
Anscombe on Brute Facts and Human Affairs
Authors: Wiseman Rachael
Published: June 2020

In ‘Modern Moral Philosophy’ Anscombe writes: ‘It is not profitable at present for us to do moral philosophy. It should be laid aside at any rate until we have an adequate philosophy of psychology, in which we are conspicuously lacking’. In consideration of this Anscombe appeals to the relation of ‘brute-relative-to’ which holds between facts and descriptions of human affairs. This paper describes the reorientation in philosophy of action that this relation aims to effect and examines the claim that this reorientation makes possible the sort of philosophy of psychology that can provide a starting point for ethics.
Political instability, institutional change and economic growth in Brazil since 1870
Authors: Campos Nauro , Karanasos Menelaos , Koutroumpis Panagiotis , Zhang Zihui
Published: June 2020

Are institutions a deep cause of economic growth? This paper tries to answer this question in a novel manner by focusing on within-country variation, over long periods of time, using a new hand-collected data set on institutions and the power-ARCH econometric framework. Focusing on the case of Brazil since 1870, our results suggest (a) that both changes in formal political institutions and informal political instability affect economic growth negatively, (b) there are important differences in terms of their short-versuslong-run behaviour, and (c) not all but just a few selected institutions affect economic growth in the long-run.
Explanatory Psychological Factors of Inconsistently Condom Use among Spanish University Students: Gender Differences
Authors: Ruiz-Palomino Estefanía , Gil-Llario María Dolores , Giménez-García Cristina , Ballester-Arnal Rafael
Published: June 2020

More than 50% of Spanish youth does not use of condoms correctly and consistently. University students are a particular population at high risk of the HIV infection, other STIs and unwanted pregnancies. The influence of psychological variables in using of condom is analyzed by gender and type of sexual relationship. A total of 256 females and 168 males were assessed (Mage= 20.62; SD = 2.16). A regression logistic analysis showed that sexual sensation seeking appears as an explanatory variable of inconsistently condom use in both females and males in vaginal intercourse, steady relationship intercourse and sex under the alcohol and other drugs effects (adjusted odds ratio between 1.095 and 1.124). Moreover, self-esteem appears as a risk factor and extraversion as a protective factor of use of condom in females. On the other hand, neuroticism reveals itself as a protective factor and fear of negative evaluation as a risk factor of use of condom in males. It is necessary to know the psychological mechanisms that underlie sexual risk behaviors for adapting interventions to individual and contextual characteristics.
ANGI — Anorexia Nervosa Genetics Initiative
Authors: Bulik Cynthia , Kennedy Martin , Wade Tracey
Published: June 2020

Identification of genetic variants associated with eating disorders is underway. The Anorexia Nervosa Genetics Initiative, an initiative of the Klarman Family Foundation, has contributed to advancing the field, yielding a large-scale genome-wide association study published in Nature Genetics. Eight genetic variants significantly associated with anorexia nervosa were identified, along with patterns of genetic correlations that suggest both psychiatric and metabolic origins of this serious and life-threatening illness. This article details the role of Professor Nick Martin in contributing to this important collaboration.
Sociopolitical Attitudes Through the Lens of Behavioral Genetics: Contributions from Dr Nicholas Martin
Authors: Verhulst Brad
Published: June 2020

Professor Nicholas (Nick) Martin spearheaded initial investigations into the genetic basis of political attitudes and behaviors, demonstrating that behaviors that are perceived as socially constructed could have a biological basis. As he showed, the typical mode of inheritance for political attitudes consists of approximately equal proportions of variance from additive genetic, shared environmental and unique environmental sources. This differs from other psychological variables, such as personality traits, which tend to be characterized by genetic and unique environmental sources of variation. By treating political attitudes as a model phenotype, Nick Martin was able to leverage the unique pattern of observed intergenerational transmission for political attitudes to reexamine the quintessential assumptions of the classical twin model. Specifically, by creatively leveraging the nuances of the genetic architecture of political attitudes, he was able to demonstrate the robustness of the equal environments assumption and suggest corrections to account for assortative mating. These advances have had a substantial impact on both the fields of political science, as well as behavioral and quantitative genetics.
The Academic Development Study of Australian Twins (ADSAT): Research Aims and Design
Authors: Larsen Sally A. , Little Callie W. , Grasby Katrina , Byrne Brian , Olson Richard K. , Coventry William L.
Published: June 2020

The Academic Development Study of Australian Twins was established in 2012 with the purpose of investigating the relative influence of genes and environments in literacy and numeracy capabilities across two primary and two secondary school grades in Australia. It is the first longitudinal twin project of its kind in Australia and comprises a sample of 2762 twin pairs, 40 triplet sets and 1485 nontwin siblings. Measures include standardized literacy and numeracy test data collected at Grades 3, 5, 7 and 9 as part of the National Assessment Program: Literacy and Numeracy. A range of demographic and behavioral data was also collected, some at multiple longitudinal time points. This article outlines the background and rationale for the study and provides an overview for the research design, sample and measures collected. Findings emerging from the project and future directions are discussed.
Deep Learning for an Improved Prediction of Rainfall Retrievals From Commercial Microwave Links
Authors: Pudashine Jayaram , Guyot Adrien , Petitjean Francois , Pauwels Valentijn R. N. , Uijlenhoet Remko , Seed Alan , Prakash Mahesh , Walker Jeffrey P.
Published: June 2020

Commercial microwave links (CMLs) have proven useful for providing rainfall information close to the ground surface. However, large uncertainties are associated with these retrievals, partly due to challenges in the type of data collection and processing. In particular, the most common case is when only minimum and maximum received signal levels (RSLs) over a given time interval (hereafter 15 min) are stored by mobile network operators. The average attenuation and the corresponding rainfall rate are then calculated based on a weighted average method using the minimum and maximum attenuation. In this study, an alternative to using a constant weighted average method is explored, based on a machine learning model trained to produce actual attenuation from minimum/maximum values. A rainfall retrieval deep learning model was designed based on a long short‐term memory (LSTM) model architecture and trained with disdrometer data in a form that is comparable to the data provided by mobile network operators. A first evaluation used only disdrometer data to mimic both attenuation from a CML and corresponding rainfall rates. For the test data set, the relative bias was reduced from 5.99% to 2.84% and the coefficient of determination (R2) increased from 0.86 to 0.97. The second evaluation used this disdrometer‐trained LSTM to retrieve rainfall rates from an actual CML located nearby the disdrometer. A significant improvement in the overall rainfall estimation compared to existing microwave link attenuation models was observed. The relative bias reduced from 7.39% to −1.14% and the R2 improved from 0.71 to 0.82.
Pre-deployment risk factors for PTSD in active-duty personnel deployed to Afghanistan: a machine-learning approach for analyzing multivariate predictors
Authors: Schultebraucks Katharina , Qian Meng , Abu-Amara Duna , Dean Kelsey , Laska Eugene , Siegel Carole , Gautam Aarti , Guffanti Guia , Hammamieh Rasha , Misganaw Burook , Mellon Synthia H. , Wolkowitz Owen M. , Blessing Esther M. , Etkin Amit , Ressler Kerry J. , Doyle Francis J. , Jett Marti , Marmar Charles R.
Published: June 2020

Active-duty Army personnel can be exposed to traumatic warzone events and are at increased risk for developing post-traumatic stress disorder (PTSD) compared with the general population. PTSD is associated with high individual and societal costs, but identification of predictive markers to determine deployment readiness and risk mitigation strategies is not well understood. This prospective longitudinal naturalistic cohort study—the Fort Campbell Cohort study—examined the value of using a large multidimensional dataset collected from soldiers prior to deployment to Afghanistan for predicting post-deployment PTSD status. The dataset consisted of polygenic, epigenetic, metabolomic, endocrine, inflammatory and routine clinical lab markers, computerized neurocognitive testing, and symptom self-reports. The analysis was computed on active-duty Army personnel (N = 473) of the 101st Airborne at Fort Campbell, Kentucky. Machine-learning models predicted provisional PTSD diagnosis 90–180 days post deployment (random forest: AUC = 0.78, 95% CI = 0.67–0.89, sensitivity = 0.78, specificity = 0.71; SVM: AUC = 0.88, 95% CI = 0.78–0.98, sensitivity = 0.89, specificity = 0.79) and longitudinal PTSD symptom trajectories identified with latent growth mixture modeling (random forest: AUC = 0.85, 95% CI = 0.75–0.96, sensitivity = 0.88, specificity = 0.69; SVM: AUC = 0.87, 95% CI = 0.79–0.96, sensitivity = 0.80, specificity = 0.85). Among the highest-ranked predictive features were pre-deployment sleep quality, anxiety, depression, sustained attention, and cognitive flexibility. Blood-based biomarkers including metabolites, epigenomic, immune, inflammatory, and liver function markers complemented the most important predictors. The clinical prediction of post-deployment symptom trajectories and provisional PTSD diagnosis based on pre-deployment data achieved high discriminatory power. The predictive models may be used to determine deployment readiness and to determine novel pre-deployment interventions to mitigate the risk for deployment-related PTSD.
RNA-binding proteins tristetraprolin and human antigen R are novel modulators of podocyte injury in diabetic kidney disease
Authors: Guo Jia , Lei Min , Cheng Fei , Liu Yong , Zhou Mengwen , Zheng Wen , Zhou Yali , Gong Rujun , Liu Zhangsuo
Published: June 2020

Diabetic kidney disease (DKD) is one of the most common complications of diabetes, and the most common cause of end-stage renal disease, for which no effective therapies are yet available. RNA-binding proteins (RBPs) play a pivotal role in epigenetic regulation; tristetraprolin (TTP) and human antigen R (HuR) competitively bind cytokine mRNAs, exert contrasting effects on RNA stability, and drive inflammation. However, RBPs’ roles in diabetes-related glomerulopathy are poorly understood. Herein, we investigated whether TTP and HuR are involved in post-transcriptional regulation of podocytopathic molecules and inflammatory cytokines in DKD. In DKD patients and db/db mice, TTP expression was significantly decreased and HuR expression was increased in glomerular podocytes, concurrent with podocyte injury, histological signs of DKD, and augmented glomerular expression of interleukin (IL)-17 and claudin-1, which are targets of TTP and HuR, as evidenced by RNA immunoprecipitation. In cultured podocytes, exposure to high ambient glucose amplified HuR expression and repressed TTP expression, upregulated IL-17 and claudin-1, and promoted podocyte injury. Thus, TTP hypoactivity or HuR hyperactivity is sufficient and essential to diabetic podocytopathy. Moreover, in silico analysis revealed that several kinases govern phosphorylation and activation of TTP and HuR, and glycogen synthase kinase (GSK)-3β activated both TTP and HuR, which harbor putative GSK-3β consensus phosphorylation motifs. Treatment of db/db mice with a small molecule inhibitor of GSK-3β abrogated the changes in TTP and HuR in glomeruli and mitigated the overexpression of their target genes (IL-17, claudin-1, B7-1, and MCP-1) thus also mitigating proteinuria and DKD pathology. Our study indicates that TTP and HuR are dysregulated in DKD via a GSK-3β-mediated mechanism and play crucial roles in podocyte injury through post-transcriptional regulation of diverse genes. It also provides novel insights into DKD’s pathophysiology and identifies potential therapeutic targets.
The impact of learning on perceptual decisions and its implication for speed-accuracy tradeoffs
Authors: Mendonça André G. , Drugowitsch Jan , Vicente M. Inês , DeWitt Eric E. J. , Pouget Alexandre , Mainen Zachary F.
Published: June 2020

In standard models of perceptual decision-making, noisy sensory evidence is considered to be the primary source of choice errors and the accumulation of evidence needed to overcome this noise gives rise to speed-accuracy tradeoffs. Here, we investigated how the history of recent choices and their outcomes interact with these processes using a combination of theory and experiment. We found that the speed and accuracy of performance of rats on olfactory decision tasks could be best explained by a Bayesian model that combines reinforcement-based learning with accumulation of uncertain sensory evidence. This model predicted the specific pattern of trial history effects that were found in the data. The results suggest that learning is a critical factor contributing to speed-accuracy tradeoffs in decision-making, and that task history effects are not simply biases but rather the signatures of an optimal learning strategy.
Cellular census of human fibrosis defines functionally distinct stromal cell types and states
Authors: Layton Thomas B. , Williams Lynn , McCann Fiona , Zhang Mingjun , Fritzsche Marco , Colin-York Huw , Cabrita Marisa , Ng Michael T. H. , Feldmann Marc , Sansom Stephen N. , Furniss Dominic , Xie Weilin , Nanchahal Jagdeep
Published: June 2020

Fibrotic disorders are some of the most devastating and poorly treated conditions in developed nations, yet effective therapeutics are not identified for many of them. A major barrier for the identification of targets and successful clinical translation is a limited understanding of the human fibrotic microenvironment. Here, we construct a stromal cell atlas of human fibrosis at single cell resolution from patients with Dupuytren’s disease, a localized fibrotic condition of the hand. A molecular taxonomy of the fibrotic milieu characterises functionally distinct stromal cell types and states, including a subset of immune regulatory ICAM1+ fibroblasts. In developing fibrosis, myofibroblasts exist along an activation continuum of phenotypically distinct populations. We also show that the tetraspanin CD82 regulates cell cycle progression and can be used as a cell surface marker of myofibroblasts. These findings have important implications for targeting core pathogenic drivers of human fibrosis.
IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses
Authors: Evnouchidou Irini , Chappert Pascal , Benadda Samira , Zucchetti Andres , Weimershaus Mirjana , Bens Marcelle , Caillens Vivien , Koumantou Despoina , Lotersztajn Sophie , van Endert Peter , Davoust Jean , Guermonprez Pierre , Hivroz Claire , Gross David A. , Saveanu Loredana
Published: June 2020

T cell receptor (TCR) activation is modulated by mechanisms such as TCR endocytosis, which is thought to terminate TCR signalling. Here we show that, upon internalization, TCR continues to signal from a set of specialized endosomes that are crucial for T cell functions. Mechanistically, TCR ligation leads to clathrin-mediated internalization of the TCR-CD3ζcomplex, while maintaining CD3ζsignalling, in endosomal vesicles that contain the insulin responsive aminopeptidase (IRAP) and the SNARE protein Syntaxin 6. Destabilization of this compartment through IRAP deletion enhances plasma membrane expression of the TCR-CD3ζcomplex, yet compromises overall CD3ζsignalling; moreover, the integrity of this compartment is also crucial for T cell activation and survival after suboptimal TCR activation, as mice engineered with a T cell-specific deletion of IRAP fail to develop efficient polyclonal anti-tumour responses. Our results thus reveal a previously unappreciated function of IRAP-dependent endosomal TCR signalling in T cell activation.
A hypomorphic cystathionine ß-synthase gene contributes to cavefish eye loss by disrupting optic vasculature
Authors: Ma Li , Gore Aniket V. , Castranova Daniel , Shi Janet , Ng Mandy , Tomins Kelly A. , van der Weele Corine M. , Weinstein Brant M. , Jeffery William R.
Published: June 2020

Vestigial structures are key indicators of evolutionary descent, but the mechanisms underlying their development are poorly understood. This study examines vestigial eye formation in the teleost Astyanax mexicanus, which consists of a sighted surface-dwelling morph and multiple populations of blind cave morphs. Cavefish embryos initially develop eyes, but they subsequently degenerate and become vestigial structures embedded in the head. The mutated genes involved in cavefish vestigial eye formation have not been characterized. Here we identify cystathionine ß-synthase a (cbsa), which encodes the key enzyme of the transsulfuration pathway, as one of the mutated genes responsible for eye degeneration in multiple cavefish populations. The inactivation of cbsa affects eye development by increasing the transsulfuration intermediate homocysteine and inducing defects in optic vasculature, which result in aneurysms and eye hemorrhages. Our findings suggest that localized modifications in the circulatory system may have contributed to the evolution of vestigial eyes in cavefish.
High permeability sub-nanometre sieve composite MoS2 membranes
Authors: Sapkota Bedanga , Liang Wentao , VahidMohammadi Armin , Karnik Rohit , Noy Aleksandr , Wanunu Meni
Published: June 2020

Two-dimensional membranes have gained enormous interest due to their potential to deliver precision filtration of species with performance that can challenge current desalination membrane platforms. Molybdenum disulfide (MoS2) laminar membranes have recently demonstrated superior stability in aqueous environment to their extensively-studied analogs graphene-based membranes; however, challenges such as low ion rejection for high salinity water, low water flux, and low stability over time delay their potential adoption as a viable technology. Here, we report composite laminate multilayer MoS2 membranes with stacked heterodimensional one- to two-layer-thick porous nanosheets and nanodisks. These membranes have a multimodal porous network structure with tunable surface charge, pore size, and interlayer spacing. In forward osmosis, our membranes reject more than 99% of salts at high salinities and, in reverse osmosis, small-molecule organic dyes and salts are efficiently filtered. Finally, our membranes stably operate for over a month, implying their potential for use in commercial water purification applications.
Toffee – a highly efficient, lossless file format for DIA-MS
Authors: Tully Brett
Published: June 2020

The closed nature of vendor file formats in mass spectrometry is a significant barrier to progress in developing robust bioinformatics software. In response, the community has developed the open mzML format, implemented in XML and based on controlled vocabularies. Widely adopted, mzML is an important step forward; however, it suffers from two challenges that are particularly apparent as the field moves to high-throughput proteomics: large increase in file size, and a largely sequential I/O access pattern. Described here is ‘toffee’, an open, random I/O format backed by HDF5, with lossless compression that gives file sizes similar to the original vendor format and can be reconverted back to mzML without penalty. It is shown that mzML and toffee are equivalent when processing data using OpenSWATH algorithms, in additional to novel applications that are enabled by new data access patterns. For instance, a peptide-centric deep-learning pipeline for peptide identification is proposed. Documentation and examples are available at https://toffee.readthedocs.io, and all code is MIT licensed at https://bitbucket.org/cmriprocan/toffee.
Spatially resolved filament wavefront dynamics
Authors: Thul Daniel , Richardson Martin , Rostami Fairchild Shermineh
Published: June 2020

Spatially resolved wavefront measurements are presented during nonlinear self-collapse and provide the first detailed characterization of wavefront evolution during filament formation. The wavefront dynamics of key nonlinear processes including Kerr self-focusing, ionization and plasma defocusing, and dynamic spatial replenishment are identified and resolved in both the filament core and reservoir regions. These results are analyzed and interpreted with respect to numerical simulations and provide insight into fundamental aspects of filamentation. They also inform applications based on phase manipulation, such as external beam guiding, and present a new method for measuring the nonlinear index of refraction, n2.
Silver nanoparticle conductive inks: synthesis, characterization, and fabrication of inkjet-printed flexible electrodes
Authors: Fernandes Iara J. , Aroche Angélica F. , Schuck Ariadna , Lamberty Paola , Peter Celso R. , Hasenkamp Willyan , Rocha Tatiana L. A. C.
Published: June 2020

Flexible electronics can be developed with a low-cost and simple fabrication process while being environmentally friendly. Conductive silver inks have been the most applied material in flexible substrates. This study evaluated the performance of different conductive ink formulations using silver nanoparticles by studying the material properties, the inkjet printing process, and application based on electrical impedance spectroscopy using a buffer solution. Silver nanoparticles synthesis was carried out through chemical reduction of silver nitrate; then, seven conductive ink formulations were produced. Properties such as resistivity, viscosity, surface tension, adhesion, inkjet printability of the inks, and electrical impedance of the printed electrodes were investigated. Curing temperature directly influenced the electrical properties of the inks. The resistivity obtained varied from 3.3 × 100 to 5.6 × 10−06 Ω.cm. Viscosity ranged from 3.7 to 7.4 mPa.s, which is suitable for inkjet printing fabrication. By using a buffer solution as an analyte, the printed electrode pairs presented electrical impedance lower than 200 Ω for all the proposed designs, demonstrating the potential of the formulated inks for utilization in flexible electronic devices for biological sensing applications.
Proteome and transcriptome reveal the involvement of heat shock proteins and antioxidant system in thermotolerance of Clematis florida
Authors: Jiang Changhua , Bi Yuke , Mo Jianbin , Zhang Ruyao , Qu Mingnan , Feng Shucheng , Essemine Jemaa
Published: June 2020

Clematis florida Thun (CfT) is an ornamental and medicinal plant. It is a cold resistant but heat sensitive species and deserves to be further investigated to improve its adaptability to heat stress. Exploring the molecular mechanism potential via an omic-analysis constitutes a promising approach towards improving heat tolerance of CfT. Two CfT lines, heat resistance (HR) and heat sensitive (HS), with differential thermotolerance capacities were used for the integrative analyses of proteomics and transcriptomes. Transcriptomes analysis showed that various pathways were significantly enriched including plant hormone signal transduction and carbon fixation pathways in prokaryotes. Proteomics study revealed the enrichment of some other pathways comprising antioxidant activity and carbohydrates metabolism. Based on combined transcriptomes and proteomics analyses and following heat stress treatment, a total of 1724 annotated genes were overlapped between both CfT lines. Particularly, 84 differential expressed genes (DEGs) were overlapped in both CfT lines. Fifteen out of these 84 genes were up-regulated solely for HR line (PS) but not for HS one (SG). This strongly suggests a potential prominent role for these genes in the thermotolerance process in PS line. We corroborate that two Hsps (Hsp18 and Hsp70) out of 20 detected proteins with higher expression levels in PS than in SG based on either global transcripts or proteins levels. According to the transcriptomes and proteomics analyses, 6 proteins and their corresponding genes were found to be significantly abundant in HR line (PS). Data are available via ProteomeXchange with identifier PXD018192. The expressions levels of these 6 genes were checked also for both CfT lines to evaluate their potential contributions in the heat tolerance process. Thus, their expression levels were approximately 2~4 times higher in HR than in HS line. We provided as well a representative schematic model to highlight the key genes involved in ROS scavenging and photorespiratory pathway in CfT. This model could be helpful also in understanding the mechanism of heat tolerance in CfT.
Duplication and divergence: New insights into AXR1 and AXL functions in DNA repair and meiosis
Authors: Martinez-Garcia Marina , Fernández-Jiménez Nadia , Santos Juan L. , Pradillo Mónica
Published: June 2020

Rubylation is a conserved regulatory pathway similar to ubiquitination and essential in the response to the plant hormone auxin. In Arabidopsis thaliana, AUXIN RESISTANT1 (AXR1) functions as the E1-ligase in the rubylation pathway. The gene AXR1-LIKE (AXL), generated by a relatively recent duplication event, can partially replace AXR1 in this pathway. We have analysed mutants deficient for both proteins and complementation lines (with the AXR1 promoter and either AXR1 or AXL coding sequences) to further study the extent of functional redundancy between both genes regarding two processes: meiosis and DNA repair. Here we report that whereas AXR1 is essential to ensure the obligatory chiasma, AXL seems to be dispensable during meiosis, although its absence slightly alters chiasma distribution. In addition, expression of key DNA repair and meiotic genes is altered when either AXR1 or AXL are absent. Furthermore, our results support a significant role for both genes in DNA repair that was not previously described. These findings highlight that AXR1 and AXL show a functional divergence in relation to their involvement in homologous recombination, exemplifying a duplicate retention model in which one copy tends to have more sub-functions than its paralog.
Body size trends in response to climate and urbanization in the widespread North American deer mouse, Peromyscus maniculatus
Authors: Guralnick Robert , Hantak Maggie M. , Li Daijiang , McLean Bryan S.
Published: June 2020

Body size decline is hypothesized to be a key response to climate warming, including warming driven by urban heat islands. However, urbanization may also generate selective gradients for body size increases in smaller endotherms via habitat fragmentation. Here we utilize a densely sampled, multi-source dataset to examine how climate and urbanization affect body size of Peromyscus maniculatus (PEMA), an abundant rodent found across North America. We predicted PEMA would conform to Bergmann’s Rule, e.g. larger individuals in colder climates, spatially and temporally. Hypotheses regarding body size in relation to urbanization are less clear; however, with increased food resources due to greater anthropogenic activity, we expected an increase in PEMA size. Spatial mixed-models showed that PEMA conform to Bergmann’s Rule and that PEMA were shorter in more urbanized areas. With the inclusion of decade in mixed-models, we found PEMA mass, but not length, is decreasing over time irrespective of climate or population density. We also unexpectedly found that, over time, smaller-bodied populations of PEMA are getting larger, while larger-bodied populations are getting smaller. Our work highlights the importance of using dense spatiotemporal datasets, and modeling frameworks that account for bias, to better disentangle broad-scale climatic and urbanization effects on body size.
Ancient DNA of Guinea Pigs (Cavia spp.) Indicates a Probable New Center of Domestication and Pathways of Global Distribution
Authors: Lord E. , Collins C. , deFrance S. , LeFebvre M. J. , Pigière F. , Eeckhout P. , Erauw C. , Fitzpatrick S. M. , Healy P. F. , Martínez-Polanco M. F. , Garcia J. L. , Ramos Roca E. , Delgado M. , Sánchez Urriago A. , Peña Léon G. A. , Toyne J. M. , Dahlstedt A. , Moore K. M. , Laguer Diaz C. , Zori C. , Matisoo-Smith E.
Published: June 2020

Guinea pigs (Caviaspp.) have a long association with humans. From as early as 10,000 years ago they were a wild food source. Later, domesticatedCavia porcelluswere dispersed well beyond their native range through pre-Columbian exchange networks and, more recently, widely across the globe. Here we present 46 complete mitogenomes of archaeological guinea pigs from sites in Peru, Bolivia, Colombia, the Caribbean, Belgium and the United States to elucidate their evolutionary history, origins and paths of dispersal. Our results indicate an independent centre of domestication ofCaviain the eastern Colombian Highlands. We identify a Peruvian origin for the initial introduction of domesticated guinea pigs (Cavia porcellus) beyond South America into the Caribbean. We also demonstrate that Peru was the probable source of the earliest known guinea pigs transported, as part of the exotic pet trade, to both Europe and the southeastern United States. Finally, we identify a modern reintroduction of guinea pigs to Puerto Rico, where local inhabitants use them for food. This research demonstrates that the natural and cultural history of guinea pigs is more complex than previously known and has implications for other studies regarding regional to global-scale studies of mammal domestication, translocation, and distribution.
Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence
Authors: Prakash John , Mishra Ashok Kumar
Published: June 2020

Qualitative and quantitative display of multiple fluorescent analytes is made simple and reliable in this micelle assisted methodology. The adopted method involves micelle assisted evincing of ppb level of PAHs in water; measurement of total fluorescence (white light excitation fluorescence, WLEF) and data deciphering using multivariate analysis. This protocol yields sensitive and accurate quantification of the cancerous pollutants (PAHs) in aqueous media with Limit of Quantification of the order 1–10 μg/L and accuracy of >98%. The use of WLEF enables the simultaneous acquisition of fluorescence signatures of all the PAHs. It has the additional advantage of being portable, layman-friendly and cost-effective. The optimized amount of surfactants for the simultaneous extraction of PAHs from real samples was estimated as 27.8 mg (19.3 mM) of SDS and 9.1 mg (5 mM) of CTAB. Also, the analytical fidelity of the quantification such as percentage recovery (98 ± 2%), linear dynamic range (2–250 μg/L), RMSEP (<0.5), etc. explains the veracity of methodology.
Ganoderma lucidum aqueous extract prevents hypobaric hypoxia induced memory deficit by modulating neurotransmission, neuroplasticity and maintaining redox homeostasis
Authors: Sharma Purva , Tulsawani Rajkumar
Published: June 2020

Oxidative stress due to hypobaric hypoxia at extreme altitudes causes severe neuronal damage and irreversible cognitive loss. Owing to contraindications of current drug therapies, the aim of the study was to investigate memory enhancing potential of aqueous extract ofGanoderma lucidum(GLAQ) and underlying neuroprotective mechanism using rat hypobaric hypoxia test model. Rats exposed to hypobaric hypoxia showed deranged spatial memory in morris water maze test with hippocampal damage and vasogenic cerebral edema. All these changes were prevented with GLAQ treatment. Blood and biochemical analysis revealed activation of hypoxic ventilatory response, red blood cells induction, reversal of electrolyte and redox imbalance, and restoration of cellular bioenergetic losses in GLAQ treated animals. Notably, GLAQ treatment ameliorated levels of neurotransmitters (catecholamines, serotonin, glutamate), prevented glucocorticoid and α-synuclein surge, improved neuroplasticity by upregulating CREB/p-CREB/BDNF expression via ERK1/ERK2 induction. Further, restoration of nuclear factor erythroid 2-related factor with stabilization of hypoxia inducible factors and inflammatory markers were evidenced in GLAQ treated rats which was additionally established in gene reporter array using an alternative HT22 cell test model. Conclusively, our studies provide novel insights into systemic to molecular level protective mechanism by GLAQ in combating hypobaric hypoxia induced oxidative stress and memory impairment.
Motor imagery training speeds up gait recovery and decreases the risk of falls in patients submitted to total knee arthroplasty
Authors: Zapparoli Laura , Sacheli Lucia Maria , Seghezzi Silvia , Preti Matteo , Stucovitz Elena , Negrini Francesco , Pelosi Catia , Ursino Nicola , Banfi Giuseppe , Paulesu Eraldo
Published: June 2020

With Motor imagery (MI), movements are mentally rehearsed without overt actions; this procedure has been adopted in motor rehabilitation, primarily in brain-damaged patients. Here we rather tested the clinical potentials of MI in purely orthopaedic patients who, by definition, should maximally benefit of mental exercises because of their intact brain. To this end we studied the recovery of gait after total knee arthroplasty and evaluated whether MI combined with physiotherapy could speed up the recovery of gait and even limit the occurrence of future falls. We studied 48 patients at the beginning and by the end of the post-surgery residential rehabilitation program: half of them completed a specific MI training supported by computerized visual stimulation (experimental group); the other half performed a non-motoric cognitive training (control group). All patients also had standard physiotherapy. By the end of the rehabilitation, the experimental group showed a better recovery of gait and active knee flexion-extension movements, and less pain. The number of falls or near falls after surgery was significantly lower in the experimental group. These results show that MI can improve gait abilities and limit future falls in orthopaedic patients, without collateral risks and with limited costs.
Human metaphase chromosome consists of randomly arranged chromatin fibres with up to 30-nm diameter
Authors: Wako Toshiyuki , Yoshida Akiyo , Kato Jun , Otsuka Yuji , Ogawa Shinichi , Kaneyoshi Kohei , Takata Hideaki , Fukui Kiichi
Published: June 2020

During cell division, mitotic chromosomes assemble and are equally distributed into two new daughter cells. The chromosome organisation of the two chromatids is essential for even distribution of genetic materials. Although the 11-nm fibre or nucleosome structure is well-understood as a fundamental fibrous structure of chromosomes, the reports on organisation of 30-nm basic chromatin fibres have been controversial, with debates on the contribution of 30-nm or thicker fibres to the higher order inner structure of chromosomes. Here, we used focused ion beam/scanning electron microscopy (FIB/SEM) to show that both 11-nm and 30-nm fibres are present in the human metaphase chromosome, although the higher-order periodical structure could not be detected under the conditions employed. We directly dissected the chromosome every 10-nm and observed 224 cross-section SEM images. We demonstrated that the chromosome consisted of chromatin fibres of an average diameter of 16.9-nm. The majority of the chromatin fibres had diameters between 5 and 25-nm, while those with 30-nm were in the minority. The reduced packaging ratio of the chromatin fibres was detected at axial regions of each chromatid. Our results provide a strong basis for further discussions on the chromosome higher-order structure.