bluuebunny/crossref_metadata_2025 huggingface dataset:
Influência do agente de união na resistência ao cisalhamento da interface titânio/polímero de vidro
Authors: Oyafuso Denise Kanashiro , Bottino Marco Antonio , Itinoche Marcos Koiti , Nasraui Anna Paula , Costa Elza Maria Valadares da
Published: April 2005

Há poucas informações em relação à efetividade da união entre compósitos e superfícies metálicas. Este estudo avaliou a influência de dois agentes de união na resistência ao cisalhamento da interface formada pelo titânio comercialmente puro (Ti cp) e um compósito (Artglass/ Heraeus Kulzer). Vinte estruturas metálicas (4mm de diâmetro e 5mm de altura) de titânio grau 1 foram fundidas, jateadas com partículas de óxido de alumínio (250ìm) e separadas em dois grupos. Para cada grupo foi utilizado um agente de união (Sistema Siloc-Pre ou Retention Flow) como procedimento anterior à aplicação do material opaco. Sobre este foram aplicados camadas do polímero referente à dentina com o auxílio de uma matriz de teflon. A manipulação e polimerização foram realizadas de acordo com as recomendações do fabricante. Os corpos-de-prova foram armazenados em água destilada por 24 horas à 37ºC e termociclados (5º e 55ºC/ 500ciclos). O teste de resistência ao cisalhamento foi realizado em uma máquina de ensaios universal (Instron) com velocidade de 5mm/min. Os dados foram tratados estatisticamente pela Análise de Variância one-way (á=0,5). Os resultados indicaram que o sistema adesivo Retention Flow foi estatisticamente melhor do que o Siloc, com médias de 20,74MPa e 11,65MPa, respectivamente. Foi possível concluir que o sistema de união influenciou na adesão entre o titânio grau I e o polímero de vidro estudado.
Análise histológica, radiográfica e do perfil de imunoglobulinas após implantação de enxerto de osso esponjoso bovino desmineralizado em bloco em músculo de ratos
Authors: Sanada Jefferson Tomio , Rodrigues José Gustavo Ribeiro , Canova Giovana Calicchio , Cestari Tânia Mary , Taga Eulazio Mikio , Taga Rumio , Buzalaf Marília Afonso Rabelo , Granjeiro José Mauro
Published: April 2005

O objetivo deste trabalho foi avaliar a biocompatibilidade de blocos de enxerto de osso bovino esponjoso acelular e desmineralizado (Gen-Ox®, Baumer S.A.). Um bloco cilíndrico (5x12mm) de material de enxerto foi implantado em músculo abdutor da coxa de 30 ratos, sendo os animais sacrificados 3, 7, 14, 21 e 28 dias (n=6) após as cirurgias. Após a tomada das radiografias, as peças foram removidas para o processamento histológico. A análise histológica mostrou que nos períodos de 3 e 7 dias foi evidenciado um processo inflamatório agudo, caracterizado pela presença de neutrófilos, reabsorção do coágulo sanguíneo e angiogênese. Entre 14 e 21 dias, verificou-se a reabsorção da matriz implantada por células mononucleadas, raras células gigantes e sua substituição por tecido conjuntivo fibroso rico em vasos e células. Aos 28 dias, na maioria dos casos, observou-se apenas pequenos fragmentos de matriz implantada envolto por tecido conjuntivo característico da região. Radiograficamente, não se notou evidências de mineralização. Com base nos resultados obtidos concluímos que o enxerto de matriz de osso esponjoso bovino desmineralizado em bloco é biocompatível quando implantado em tecido conjuntivo intramuscular de ratos, sendo absorvido e substituído por tecido conjuntivo característico da região, sem qualquer indício de ocorrência de osteogênese ectópica.
Effect of sodium hypochlorite on the bond strength of an adhesive system to superficial and deep dentin
Authors: Uceda-Gómez Ninoshka , Reis Alessandra , Carrilho Marcela Rocha de Oliveira , Loguercio Alessandro Dourado , Rodriguez Filho Leonardo Eloy
Published: April 2005

The objective of this study was to evaluate the bond strength to superficial (SU) and deep (D) dentin, accessed via apical (DA) or occlusal (DO), using One-Step adhesive system applied according to the manufacturer's instructions (C) or following deproteinization with 10% sodium hypochlorite (H) for 60s, after acid etching. Three sound extracted human molars were prepared for each experimental condition. Restorations were performed using Z100 in 2mm increments, each one being light-cured for 40s. Teeth were longitudinally sectioned to obtain stick-shaped specimens with a cross-sectional area of 0.8mm², which were submitted to bond strength test (0.6mm/min). Results obtained after statistical analysis using a two-way ANOVA (substrate vs. surface treatment) and Tukey's test were: SU (35.4 ± 12.3), DO (26.5 ± 8.5), DA (26.1 ± 10.2) following conventional surface treatment, and SU (28.82 ± 12.7), DO (24.3 ± 8.3) and DA (23.5 ± 8.5), after surface treatment using sodium hypochlorite. The interaction of the factors was not significant (p>0.05). However, the main factors were significant (p<0.05). Mean bond strength values in superficial dentin were statistically superior to deep dentin (p<0.05), although no statistically significant difference was observed between the two via of access to deep dentin (SU>DO=DA). The conventional surface treatment resulted in higher bond strength values than the sodium hypochlorite treatment (p<0.05). It was concluded: 1) bond strength values were higher in superficial dentin, 2) no difference was found between the two deep substrate preparations, and 3) the application of sodium hypochlorite following dentin acid etching may reduce bond strengths.
Quantitative analyses of maxillary sinus using computed tomography
Authors: Perella Andréia , Rocha Sara dos Santos , Cavalcanti Marcelo de Gusmão Paraiso
Published: April 2005

The aim of this study was to evaluate the precision and accuracy of linear measurements of maxillary sinus made in tomographic films, by comparing with 3D reconstructed images. Linear measurements of both maxillary sinus in computed tomography CT of 17 patients, with or without lesion by two calibrated examiners independently, on two occasions, with a single manual caliper. A third examiner has done the same measurements electronically in 3D-CT reconstruction. The statistical analysis was performed using ANOVA (analyses of variance). Intra-observer percentage error was little in both cases, with and without lesion; it ranged from 1.14% to 1.82%. The inter-observer error was a little higher reaching a 2.08% value. The accuracy presented a higher value. The perceptual accuracy error was higher in samples, which had lesion compared to that which had not. CT had provided adequate precision and accuracy for maxillary sinus analyses. The precision in cases with lesion was considered inferior when compared to that without lesion, but it can't affect the method efficacy.
Calcium hydroxide: study based on scientific evidences
Authors: Estrela Carlos , Holland Roberto
Published: April 2005

The characteristics of calcium hydroxide come from its dissociation into calcium and hydroxyl ions. The action of these ions on tissues and bacteria explains the biological and antimicrobial properties of this substance. Under the conditions of this retrospective of literature, based on scientific evidences, it is possible to state that: 1. Dentin is considered the best pulpal protective, and calcium hydroxide has proved, through numerous studies, its capability of inducing the formation of a mineralized bridge over pulpal tissue. 2. It is necessary, whenever possible, to provide time for calcium hydroxide paste to manifest its potential of action on the microorganisms present in endodontic infections. The maintenance of a high concentration of hydroxyl ions can change bacteria enzymatic activity and promote its inactivation. 3. The site of action of hydroxyl ions of calcium hydroxide includes the enzymes in the cytoplasmic membrane. This medication has a large scope of action, and therefore is effective on a wide range of microorganisms, regardless their metabolic capability. In microbial world, cytoplasmic membranes are similar, irrespective from microorganisms morphological, tinctorial and respiratory characteristics, which means that this medication has a similar effect on aerobic, anaerobic, Gram-positive and Gram-negative bacteria. 4. Calcium hydroxide as temporary dressing used between appointments promotes better results on the periapical healing process than the treatment in one appointment.
Análise do perfil profissional de cirurgiões-dentistas graduados na Faculdade de Odontologia de Bauru- USP entre os anos de 1996 e 2000
Authors: Bastos José Roberto de Magalhães , Aquilante Aline Guerra , Almeida Beatriz Simões de , Lauris José Roberto Pereira , Bijella Vitoriano Truvijo
Published: April 2005

Os objetivos do presente estudo foram determinar o perfil profissional dos 248 cirurgiões-dentistas graduados na Faculdade de Odontologia de Bauru - USP entre 1996 e 2000, constatar se há a adoção de medidas educativo-preventivas de caráter coletivo por parte destes profissionais e determinar o grau de satisfação profissional, através de um questionário contendo questões abertas e de múltipla escolha. O índice de retorno foi de 39,5%. A análise dos resultados revelou que os cirurgiões-dentistas eram predominantemente do gênero masculino, escolheram a Odontologia como profissão por afinidade e consideraram bom o curso de Graduação. Os resultados demonstraram que os profissionais ainda não despertaram para a importância da educação e da prevenção, principalmente em nível coletivo. Concluiu-se que o perfil predominante do profissional é aquele que trabalha no consultório particular próprio (38,8%) ou por porcentagem (25,5%), sendo que 26,5% atende em algum Convênio ou Cooperativa e apenas 12,2% trabalham na rede pública. Embora 63,3% tenham afirmado que se sentem realizados profissionalmente, apenas 12,2% estão financeiramente realizados. Uma das maiores dificuldades enfrentadas pelos cirurgiões-dentistas é a alta competitividade e saturação do mercado de trabalho.
Fluoride content of several breakfast cereals and snacks found in Brazil
Authors: Cardoso Vanessa Eid da Silva , Olympio Kelly Polido Kaneshiro , Granjeiro José Mauro , Buzalaf Marília Afonso Rabelo
Published: April 2005

Breakfast cereals and snacks are foodstuffs highly appreciated by children, and the possibility that they contain substantial amounts of fluoride, associated with their widespread consume, may make them important contributors to the total daily fluoride intake. The aim of this study was to analyze the fluoride content on several breakfast cereals (A) and snacks (B) purchased in Brazil. The analysis were made after HMDS-facilitated diffusion (Taves) using the ion-specific electrode (9609). Mean fluoride concentrations ± SD (range, unit mg F/g) were: A= 0.76 ± 0.60 (0.08-1.86, n=15) and B= 0.32 ± 0.09 (0.22-0.55, n=18). Our results suggest that the total amount of fluoride available in some products may contribute to the total daily fluoride intake. The product labels should provide information on their fluoride content to prevent fluorosis at the age of risk.
Assement of the fluoride concentration and pH in different mouthrinses on the brazilian market
Authors: Delbem Alberto Carlos Botazzo , Sassaki Kikue Takebayashi , Castro Alessandra Maia de , Pinto Leila Maria Cesário Pereira , Bergamaschi Maurício
Published: April 2005

In this study, the fluoride concentration and pH of 14 commercial brands of mouthrinses were assessed in order to compare them with the values expressed on the labels and with those established by the National Sanitary Surveillance Agency. Forty-two (42) products were obtained from three places, with different manufacturing batches. The fluoride concentration was determined in diluted solutions, using a combined specific electrode for fluoride ion (9609 BN Orion Research) and ion analyzer (290 A Orion Research). The results showed that 50% of the solutions had statistically significant differences, with higher fluoride concentrations than those expressed on the labels, and the pH ranged from 4.23 to 7.34, but only one of the products registered the pH value on its label.
Surface roughness average and scanning electron microscopic observations of resin luting agents
Authors: Mondelli Rafael Francisco Lia , Prakki Anuradha , Cilli Renato , Navarro Maria Fidela de Lima , Mondelli José
Published: April 2005

The objective of this work was to evaluate the surface roughness changes of three current resin cements after tooth brushing simulation, as well as discuss its relation with scanning electron microscopic observations. The materials employed were Enforce Sure Cure (Dentsply), Rely X (3M-ESPE) and Variolink II (Vivadent). They were subjected to brushing abrasion (100,000 strokes for each specimen) and the surface roughness alterations (before and after strokes) were detected. For each roughness test condition, specimens were coated with gold-palladium and observed on a DSM 900 Zeiss scanning electron microscope. Roughness changes values (Ra) were statistically increased after brushing strokes. Based on the microscopic observations and roughness changes analysis, all cements studied became rougher after brushing strokes.
Influence of the deflasking delay time on the displacements of maxillary denture teeth
Authors: Consani Rafael Leonardo Xediek , Mesquita Marcelo Ferraz , Sinhoreti Mário Alexandre Coelho , Consani Simonides
Published: April 2005

The purpose of this study was to investigate the displacement of teeth occurring in dentures following storage in water at a temperature of 37ºC. Ten maxillary dentures were constructed with Classico heat-cured acrylic resin using conventional metal flasks. Metallic reference pins were placed on the incisal border of the central incisors (I), buccal cusp of the first premolars (PM), and mesiobuccal cusp of the second molars (M). The acrylic resin was polymerized twelve hours after final flask closure in a water cycle at 74ºC for 9 hours. The flasks were removed from the thermo-curing unit after water cooling and then bench stored for 3 hours. After deflasking and finishing, the dentures were stored in water at a temperature of 37ºC for periods of 7, 30, and 90 days. Following deflasking and each storage period tested, the I-I (incisor to incisor), PM-PM (premolar to premolar), and M-M (molar to molar) transverse distances, and LI-LM (left incisor to left molar) and RI-RM (right incisor to right molar) anteroposterior distances were measured with a STM Olympus microscope, with accuracy of 0.0005 mm. Colleted data were submitted to ANOVA and Tukey test (5%). No statistically significant difference was observed in the displacement of teeth in the transverse and anteroposterior distances.
Management of chemo- and radiotherapy induced oral mucositis with low-energy laser: initial results of A.C. Camargo Hospital
Authors: Sandoval Renata Lazari , Koga Daniel Henrique , Buloto Lígia Schmitd , Suzuki Ricardo , Dib Luciano Lauria
Published: April 2005

Background. Oral mucositis is a common complication of some malignancies treatment, causing therapeutic modifications due to patient's debilitation, which often interferes with the prognosis of the disease. Many attempts have been made to find an optimal treatment or preventive method to minimize the severity of oral mucositis. Several studies have shown good results with the use of low-energy laser, with the aim of accelerating the process of wound healing and promoting pain relief. Methods. Patients (n=18) who developed oral mucositis during chemotherapy and/or radiotherapy were submitted to low-energy laser applications until cessation of symptoms. Mucositis severity was scored by an oral mucositis scale based on clinical features and by an oral toxicity scale from the National Cancer Institute based on the ability to swallow; pain severity was scored by subjects on a visual analogue scale before and after the applications. Results. Immediate pain relief was achieved in 66.6% of the patients after the first application. Based on the functional scale, mucositis grade III (not capable to eat solids) was reduced in 42.85% of the cases. According to the scale based on the clinical features, mucositis grade IV (ulcerative lesions) was reduced in 75% of the patients that presented this grade of mucositis at the beginning of laser therapy. Conclusions. Low-energy laser was well-tolerated and showed beneficial effects on the management of oral mucositis, improving the quality of life during the oncologic treatment.
Effect of tooth age on bond strength to dentin
Authors: Giannini Marcelo , Chaves Patrícia , Oliveira Marcelo Tavares de
Published: April 2005

This in vitro study evaluated the effect of tooth age on the tensile bond strength of Prime & Bond NT adhesive system to dentin. Human third molars from the five age groups were analyzed: A- 17 to 20yrs, B- 21 to 30yrs, C- 31 to 40yrs, D- 41 to 50yrs and E- 51 to 63yrs. The occlusal enamel was removed using a diamond saw under water cooling and the dentin surface was wet-ground with 600-grit SiC paper to obtain flat surfaces. The adhesive system was applied according to the manufacturer's instructions and a 6-mm high resin "crown" was built-up with resin composite. Teeth were stored for 24 hours in distilled water at 37ºC and prepared for micro-tensile testing. Each specimen was mounted in a testing jig attached to a universal testing machine and stressed in tension at a crosshead speed of 0.5mm/min until failure. The means of tensile bond strength were (MPa): A- 21.42 ± 7.52ª; B- 30.13 ± 10.19ª; C- 31.69 ± 11.78ª; D- 30.69 ± 8.47ª and E- 35.66 ± 9.54ª. No statistically significant difference was observed among the age groups (p > 0.05). The results suggested that the tensile bond strength of the adhesive system was not significantly affected by dentin aging.
Comparative study of the surface roughness of Ormocer-based and conventional composites
Authors: Cunha Leonardo Gonçalves , Alonso Roberta Caroline Bruschi , Santos Paulo Henrique dos , Sinhoreti Mário Alexandre Coelho
Published: April 2005

The aim of this study was to evaluate the surface roughness of two Ormocer-based resin composites before and after mechanical toothbrushing. The study compared the brands Admira and Definite with composites based on conventional monomer systems (Bis-GMA, Bis-EMA, UEDMA e TEGDMA), Z250 and A110. Eight samples of each material with 4mm in diameter and 2mm in height were prepared using a metallic mold. After 24 hours they were polished and examined with a profilometer for measurement of the initial surface roughness (Ra, mm) before mechanical toothbrushing (30,000 cycles). After toothbrushing, the samples were taken to the profilometer once again to check the final surface roughness. The results were submitted to ANOVA and Tukey test (5%). The Admira composite presented a higher mean of surface roughness before toothbrushing (0.132mm), with a statistical difference from the composite A110 (0.082mm). Definite (0.110mm) and Z250 (0.105mm) composites showed no differences between themselves or among the other composites. No statistical differences were observed after toothbrushing between the composites Definite (0.178mm), Z250 (0.187mm), Admira (0.181mm), and A110 (0.193mm). All composites showed a statistically significant increase in the surface roughness after toothbrushing.
Evaluation of low-fusing ceramic systems combined with titanium grades II and V by bending test and scanning electron microscopy
Authors: Garbelini Wilson José , Henriques Guilherme Elias Pessanha , Troia Junior Manoel , Mesquita Marcelo Ferraz , Dezan Cássia Cilene
Published: April 2005

The bond strength by three point bending strength of two metal substrates (commercially pure titanium or grade II, and Ti-6Al-4V alloy or grade V) combined to three distinct low-fusing ceramic systems (LFC) and the nature of porcelain-metal fracture by scanning electron microscopy (SEM) were evaluated. The results were compared to a combination of palladium-silver (Pd-Ag) alloy and conventional porcelain (Duceram VMK68). Sixty metal strips measuring 25x3x0.5mm were made - 30 of titanium grade II and 30 of titanium grade V, with application of the following types of porcelain: Vita Titankeramik, Triceram or Duceratin (10 specimens for each porcelain). The porcelains were bonded to the strips with dimensions limited to 8x3x1mm. The control group consisted of ten specimens Pd-Ag alloy/Duceram VMK68 porcelain. Statistical analyses were made by one-way analysis of variance (ANOVA) and Tukey test at 5% significance level. Results showed that the bond strength in control group (48.0MPa ± 4.0) was significantly higher than the Ti grade II (26.7MPa ± 4.1) and Ti grade V (25.2MPa ± 2.2) combinations. When Duceratin porcelain was applied in both substrates, Ti grade II and Ti grade V, the results were significantly lower than in Ti grade II/Vitatitankeramik. SEM analysis indicated a predominance of adhesive fractures for the groups Ti grade II and Ti grade V, and cohesive fracture for control group Pd-Ag/Duceram. Control group showed the best bond strength compared to the groups that employed LFC. Among LFC, the worst results were obtained when Duceratin porcelain was used in both substrates. SEM confirmed the results of three point bending strength.
Reverse transcription and polymerase chain reaction: principles and applications in dentistry
Authors: Santos Carlos Ferreira dos , Sakai Vivien Thiemy , Machado Maria Aparecida de Andrade Moreira , Schippers Daniela Nicole , Greene Andrew Seth
Published: April 2005

Various molecular biology techniques have become available in the last few years. One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in 1985. This method relies on the exponential amplification of specific DNA fragments, resulting in millions of copies that can serve as templates for different kinds of analyses. PCR can be preceded by a reverse transcription (RT) reaction in order to produce cDNA from RNA (RT-PCR). RT-PCR provides the possibility to assess gene transcription in cells or tissues. PCR and RT-PCR techniques have been instrumental in dental research, and show potential to be used for diagnosis as well as for treatment and prevention of many diseases (dental caries, periodontal disease, endodontic infections and oral cancer). Compared to other traditional methodologies, PCR and RT-PCR show many advantages including high specificity, sensitivity, and speed. Since PCR and RT-PCR are relatively new techniques and are not available to most students and professionals involved with dentistry, the aim of this work is to present the details of these techniques as well as dental literature reports in which they were used.
Effect of one-bottle adhesive systems on the fluoride release of a resin-modified glass ionomer
Authors: Wang Linda , Buzalaf Marília Afonso Rabelo , Atta Maria Teresa
Published: April 2005

A dhesive systems associated to resin-modified glass ionomer cements are employed for the achievement of a higher bond strength to dentin. Despite this benefit, other properties should not be damaged. This study aimed at evaluating the short-time fluoride release of a resin-modified glass ionomer cement coated with two one-bottle adhesive systems in a pH cycling system. Four combinations were investigated: G1: Vitremer (V); G2: Vitremer + Primer (VP); G3: Vitremer + Single Bond (VSB) and G4: Vitremer + Prime & Bond 2.1 (VPB). SB is a fluoride-free and PB is a fluoride-containing system. After preparation of the Vitremer specimens, two coats of the selected adhesive system were carefully applied and light-cured. Specimens were immersed in demineralizing solution for 6 hours followed by immersion in remineralizing solution for 18 hours, totalizing the 15-day cycle. All groups released fluoride in a similar pattern, with a greater release in the beginning and decreasing with time. VP showed the greatest fluoride release, followed by V, with no statistical difference. VSB and VPB released less fluoride compared to V and VP, with statistical difference. Regardless the one-bottle adhesive system, application of coating decreased the fluoride release from the resin-modified glass ionomer cements. This suggests that this combination would reduce the beneficial effect of the restorative material to the walls around the restoration.
Alkali-soluble fluoride deposition on enamel after professional application of topical fluoride in vitro
Authors: Hayacibara Mitsue Fujimaki , Leme Adriana Franco Paes , Lima Ynara Bosco de Oliveira , Gonçalves Nilza Cristina Lopez Afonso Valor , Queiroz Celso Silva , Gomes Maria José , Kozlowski Fábio Carlos
Published: April 2005

Since the efficacy of topical fluoride products is related to the fluoride (F) availability and its reactivity with enamel, this study was conducted. The F concentration of the following materials was verified: I- acidulated phosphate fluoride (APF) gel (1.23% F), II- APF foam (1.23% F) and III- Varnish (2.26% F). Forty blocks of bovine enamel were divided into 4 groups and treated according to the materials described, being one of them used as control. Loosely bound fluoride (''CaF2'') was determined on enamel after extraction with 1.0M KOH and analyzed by ion-selective electrode. Total F concentration found in gel was 12,642, in foam 12,755 and in varnish 23,183 mg F/g. All products formed statistically higher amounts of ''CaF2'' on enamel compared to the control group (p < 0.05), but the difference between them was not significant (p > 0.05). Thus, ''CaF2'' formation was not proportional to the total F content in the products, suggesting that the pH and the vehicle used are more important.
Influence of the EDTA, Nd:YAG laser and association of both on the filling of artificial lateral root canals
Authors: Moraes Fernanda Gomes de , Bramante Clovis Monteiro , Moraes Ivaldo Gomes de , Carneiro Everdan , Menezes Renato
Published: April 2005

This study aimed at evaluating the influence of EDTA, Nd:YAG laser and the combination of both for filling of artificial lateral root canals. Forty-five human mandibular premolars were employed, on which three artificial lateral root canals were prepared by means of a reamer with a similar diameter to a K file #15. The teeth were instrumented through the stepback technique employing Gates Glidden burs at the middle and cervical thirds and manual files at the apical portion, and irrigation with 1% sodium hypochloride. The teeth were divided in three groups: Group 1 -EDTA for 5 minutes; Group 2 -application of Nd:YAG laser at 15 Hz, 100 mJ and 1.5 Watts; and Group 3 - association of both. Roots were filled through the Tagger's hybrid technique, radiographed and the radiographs were digitized. Scores were assigned to the filling of the lateral root canals. Statistical analysis revealed no significant differences between the entire groups and also on the analysis of each third.
Prevalence of dental caries in 3- and 5-year-old children living in a small Brazilian City
Authors: Ueda Eunice Mitiko Ogido , Dezan Cássia Cilene , Frossard Wanda Terezinha Garbelini , Salomão Fernanda , Morita Maria Celeste
Published: April 2005

Despite the reduction in the prevalence of dental caries, very little is known about the prevalence of the disease, especially in deciduous teeth, in small Brazilian cities (<20,000 inhabitants). The aim of this study was to evaluate the prevalence of dental caries in children aged 3 and 5 years of age in the city of Cambira - State of Parana. All children that participated in the national vaccination campaign against poliomyelitis in 2002 at the two basic health units of the city were examined. The campaign covered 99.5% of the population aged 1 to 5 years. Two previously trained and calibrated dentists performed the clinical examinations after the children brushed their teeth and recorded their caries experience by means of the dmf index. The results showed that there was no statistically significant difference in the prevalence of the disease between the male and female genders and between the rural and urban zones. At the age of 3, 68.50% of the children were caries-free, compared to 31.10% at the age of 5. In the total population, 11.19% of the children accounted for 50.86% of the teeth affected by dental caries. The dmf index at 3 and 5 years in the study population was 2.10 and 3.51, respectively. These data showed that the city has not yet reached the goals proposed by the WHO for the year 2000, what indicates the need for greater investment in programs aimed to prevention and control of the disease.
Effect of surface treatments of laboratory-fabricated composites on the microtensile bond strength to a luting resin cement
Authors: Soares Carlos José , Giannini Marcelo , Oliveira Marcelo Tavares de , Paulillo Luis Alexandre Maffei Sartini , Martins Luis Roberto Marcondes
Published: April 2005

The purpose of this study was to evaluate the influence of different surface treatments on composite resin on the microtensile bond strength to a luting resin cement. Two laboratory composites for indirect restorations, Solidex and Targis, and a conventional composite, Filtek Z250, were tested. Forty-eight composite resin blocks (5.0 x 5.0 x 5.0mm) were incrementally manufactured, which were randomly divided into six groups, according to the surface treatments: 1- control, 600-grit SiC paper (C); 2- silane priming (SI); 3- sandblasting with 50 mm Al2O3 for 10s (SA); 4- etching with 10% hydrofluoric acid for 60 s (HF); 5- HF + SI; 6 - SA + SI. Composite blocks submitted to similar surface treatments were bonded together with the resin adhesive Single Bond and Rely X luting composite. A 500-g load was applied for 5 minutes and the samples were light-cured for 40s. The bonded blocks were serially sectioned into 3 slabs with 0.9mm of thickness perpendicularly to the bonded interface (n = 12). Slabs were trimmed to a dumbbell shape and tested in tension at 0.5mm/min. For all composites tested, the application of a silane primer after sandblasting provided the highest bond strength means.
Apical displacement produced by rotary nickel-titanium instruments and stainless steel files
Authors: Vanni José Roberto , Albuquerque Diana Santana de , Reiss Cristina , Baratto Filho Flares , Limongi Orlando , Della Bona Álvaro
Published: April 2005

The aim of this study was to evaluate the apical displacement produced by different rotary nickel-titanium instruments, testing the hypothesis that rotary systems with nickel-titanium instruments produce lower mean values of apical displacement than stainless steel hand instruments. A total of 100 maxillary permanent first molars were selected for the study. The mesiobuccal roots were sectioned at the top cervical third and embedded in blocks of self-curing resin. The specimens were randomly divided into 5 groups and the root canals were prepared using the following nickel-titanium instruments: Group 1 - Quantec system 2000 (Analytic Endodontics, Mexico); Group 2 - Pro-File T.0.04 (Dentsply/Maillefer, Switzerland); Group 3 - Pro-File Series 29 T.0.04 (Dentsply Tulsa, Switzerland); Group 4 - Pow-R T.0.02 (Moyco-Union Broach, USA). Specimens in Group 5 were prepared using stainless steel hand instruments Flexofile (Dentsply/Maillefer, Switzerland). All root canals were previously submitted to cervical preparation using Orifice Shaper instruments #1, 2, 3 and 4 (Dentsply/Maillefer, Switzerland). After odontometry, the remaining root canal was shaped employing increasingly larger instruments, so that the final instrument corresponded to Quantec #9, Pro-File Series 29 #6, and #35 for the other groups. Specimens in Groups 1 to 4 were prepared using an electric handpiece with 16:1 reduction at 350 rpm. The specimens in Group 5 were manually prepared. Apical displacement was measured and recorded by means of radiographic superimposition on a specific desk. Statistical analysis (ANOVA) of the results revealed that all groups presented apical displacements. Considering only the nickel-titanium instruments, Group 4 showed the lowest mean value while Groups 2 and 3 produced the highest mean apical displacement values (p<0.001).
Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
Authors: Marins Lucele Vieira , Cestari Tania Mary , Sottovia André Dotto , Granjeiro José Mauro , Taga Rumio
Published: April 2005

Over the last few years, various bone graft materials of bovine origin to be used in oromaxillofacial surgeries have entered the market. In the present study, we determined the capacity of a block organic bone graft material (Gen-ox, Baumer SA, Brazil) prepared from bovine cancellous bone to promote the repair of critical size bone injuries in rat calvaria. A transosseous defect measuring approximately 8mm in diameter was performed with a surgical trephine in the parietal bone of 25 rats. In 15 animals, the defects were filled with a block of graft material measuring 8mm in diameter and soaked in the animal's own blood, and in the other 10 animals the defects were only filled with blood clots. The calvariae of rats receiving the material were collected 1, 3 and 6 months after surgery, and those of animals receiving the blood clots were collected immediately and 6 months after surgery. During surgery, the graft material was found to be of easy handling and to adapt perfectly to the receptor bed after soaking in blood. The results showed that, in most animals treated, the material was slowly resorbed and served as a space filling and maintenance material, favoring angiogenesis, cell migration and adhesion, and bone neoformation from the borders of the lesion. However, a foreign body-type granulomatous reaction, with the presence of numerous giant cells preventing local bone neoformation, was observed in two animals of the 1-month subgroup and in one animal of the 3-month subgroup. These cases were interpreted as resulting from the absence of demineralization and the lack of removal of potential antigen factors during production of the biomaterial. We conclude that, with improvement in the quality control of the material production, block organic bone matrix will become a good alternative for bone defect repair in the oromaxillofacial region due to its high osteoconductive capacity.
Pool of bovine morphogenetic proteins and guided tissue regeneration in the treatment of intrabony periodontal defects: I- Clinical measurements
Authors: Guimarães Maria do Carmo Machado , Passanezi Euloir , Sant'Ana Adriana Campos Passanezi , Greghi Sebastião Luiz Aguiar
Published: April 2005

The purpose of this study was to evaluate the effect of the pool of bovine BMPs on the treatment of intrabony defects. The sample comprised 15 patients aged 26 to 57 years old presenting with 10 pairs of lesions of 2 or 3 walls or 2-3 walls ³5mm, located in the same type teeth (premolar or molar) and same jaw. The test defects were treated with combination of a pool of bovine bone morphogenetic and resorbable hydroxyapatite carrier (BMPs- HA), bovine demineralized bone matrix (MB) and coverage by a bovine collagen barrier membrane. The control defects were treated with MB-HA and covered by a bovine collagen membrane. The clinical measurements at six months after therapy in the test group revealed a reduction in the mean probing pocket depth (PPD) of 1.63 ± 1.41mm (B) and 1.93 ± 0.96mm (L) and a mean change in the clinical attachment level (CAL) of 1.60 ± 1.16mm (B) and 1.46 ± 0.97mm (L). The control group showed a mean reduction of PPD of 1.93 ± 1.34mm (B) and 2.0 ± 1.51mm (L) and a mean change of CAL of 1.03 ± 1.24mm (B) and 1.30 ± 1.14 mm (L). The analysis of variance (ANOVA) demonstrated that the changes in the clinical parameters were statistically significant (p<0.05). There were no significant differences between the test and controls subjects (Student's test, p<0.005). These findings suggest that the use of a pool of bovine BMPs do not provide added effects to GTR in the treatment of intrabony defects.
Influence of age, sex, plaque and smoking on periodontal conditions in a population from Bauru, Brazil
Authors: Ragghianti Mariana Schützer , Greghi Sebastião Luiz Aguiar , Lauris José Roberto Pereira , Sant'Ana Adriana Campos Passanezi , Passanezi Euloir
Published: April 2005

Epidemiology is the study of health and disease in populations, and of how these conditions are influenced by heredity, biology, physical environment, social environment, and personal behavior. There are many epidemiological studies in Brazilian population but few about the influence of some risk factors in periodontal conditions. This cross-sectional study was performed to assess the influence of age, sex, plaque and smoking on periodontal disease in a population from Bauru (Brazil). Data concerning periodontal status were collected from 380 patients in the University of São Paulo (USP). Measurements of periodontal pocket depths (PPD), clinical attachment levels (CAL), plaque index (PI) of four sites in all teeth were registered. The influence of age, sex and smoking habits on the periodontal parameters were statistically evaluated using descriptive statistical and ANOVA. The correlation between plaque and periodontal parameters was analyzed by Pearson's correlation coefficient. The results showed an increase in the mean of periodontal destruction (PPD and CAL) and a higher number of sites with severe losses with increasing age. Correlation among percentage of sites with plaque and periodontal parameters (PPD and CAL) were positive but weakly related. The male group showed significantly higher means of CAL than the female. Smokers had significantly higher PPD and CAL means than non-smokers. Aging, smoking habit, male sex, and percentage of sites with plaque were associated with a great increase of periodontal destruction, being important factors in the diagnosis of the periodontal disease in this Brazilian population.
Evaluation of the shear bond strength of the union between two CoCr-alloys and a dental ceramic
Authors: Pretti Mariana , Hilgert Edson , Bottino Marco Antônio , Avelar Rander Pereira
Published: April 2005

INTRODUCTION: Based on the importance of the integrity of the metal/ceramic interface, the purpose of this work was to evaluate the shear bond strength of the metal-ceramic union of two Co-Cr alloys (Wirobond C, Bego; Remanium 2000, Dentaurum) combined with Omega 900 ceramic (Vita Zahnfabrik). MATERIAL AND METHOD: Eleven cylindrical matrixes were made for each alloy, and the metallic portion was obtained with the lost wax casting technique with standardized waxing of 4mm of height and of 4mm of diameter. The ceramic was applied according to the manufacturer's recommendations with the aid of a teflon matrix that allowed its dimension to be standardized in the same size as the metallic portion. The specimens were submitted to the shear bond test in an universal testing machine (EMIC), with the aid of a device developed for such intention, and constant speed of 0.5mm/min. RESULTS AND CONCLUSIONS: The mean resistance was 48.387MPa for Wirobond C alloy, with standard deviation of 17.718, and 55.956MPa for Remanium 2000, with standard deviation of 17.198. No statistically significant difference was observed between the shear strength of the two metal-ceramic alloys.
Biological compatibility of some types of endodontic calcium hydroxide and glass ionomer cements
Authors: Valera Marcia Carneiro , Leonardo Mário Roberto , Consolaro Alberto , Matuda Fábio da Silva
Published: April 2005

The purpose of this work was to evaluate the biological compatibility of the Sealapex, Apexit, Sealer 26 and Ketac Endo endodontic cements. Polyethylene tubes containing these cements were implanted in the subcutaneous tissue of 40 (forty) rats. The animals were sacrificed after 14 and 90 days. A descriptive analysis of the reactions found in the connective tissue by contact with the cements was performed. The magnitude of inflammatory infiltrate, the presence and predominance of cell types and their distribution as to the filling material and reparative phenomena, such as fibroblastic and angioblastic proliferation and formation of fibrous capsules, were subjectively measured. After 90 days, all cements presented statistically significant reduction of the inflammatory reaction, presence of a fibrous tissue capsule in contact with the opening of the tubes containing the filling materials, and reduction of fibroblastic proliferation. Angioblastic proliferation decreased only for the Sealer 26 and Ketac Endo groups. All cements tested were either partially or totally phagocyted, and the mildest inflammatory response was found for the Sealer 26 group at both evaluation periods.
Candida in saliva of Brazilian hemophilic patients
Authors: Pereira Claudio Maranhão , Pires Fábio Ramôa , Corrêa Maria Elvira Pizzigatti , di Hipólito Júnior Osvaldo , Almeida Oslei Paes de
Published: April 2005

Hemophilia is a common hereditary hemorrhagic disorder, however little is known about the oral microflora of hemophilic patients. The aim of this study was to quantify the Candida and identify its species in non-stimulated saliva of hemophilic patients, and consider its relationship with clinical factors influencing Candida carriage. This study comprised evaluation of 86 hemophilic patients of the Hematology Center/UNICAMP and 43 healthy subjects as controls. All patients were submitted to anamnesis, intraoral examination and unstimulated saliva collection. Candida counts and species identification were performed in salivary samples. Candida was present in 64% of the hemophilic patients and in 44% of the healthy controls. C. albicans represented 65% and 68% of the isolated species, in hemophiliacs and control group respectively, and C. tropicalis was the second most common species in both groups. These results indicate that hemophilic patients carry Candida more frequently and in higher counts than healthy controls, independently of oral clinical parameter considered, as viral infections, complete dentures, transfusions of hemoderivatives, and salivary flow.
Effect of thermal cycling and filling technique on leakage of composite resin restorations
Authors: Kenshima Silvia , Grande Rosa Helena Miranda , Singer Julio da Motta , Ballester Rafael Yagüe
Published: April 2005

The objective of this study was to evaluate in vitro the effect on leakage of two incremental filling techniques and two composite resins with different elastic modulus and similar polymerization shrinkage. Eighty Class V cavities (4x4x2mm) were prepared in bovine incisors and were randomly restored with Z-250 (Z) or Durafill VS (D) + Single Bond in axial (a) or oblique (o) increments. The restorations were divided into two groups: Not Aged - N (4-hour-storage in water at 37ºC) and Aged - A (1-week storage in water at 37ºC + 1000 x - 5º-55ºC / 1-min dwell time). The specimens were covered with 2 coats of nail varnish so that only the restoration margins were exposed to silver nitrate 50% (2h) and developed under fluorescent light (8h). After they were sectioned twice in buccal-lingual direction, the four exposed surfaces were digitized (Vidcap) and the silver nitrate penetration was measured (ImageLab) at the incisal and gingival walls. Data were analyzed by a 3-way ANOVA (Resin, Filling Technique and Aging) separately for incisal and gingival walls (alpha=0.05). Resin and Aging were statistically significant either for the incisal and the gingival walls. The microfill composite resin infiltrated more than the hybrid composite. The thermal cycling caused an overall increase in silver nitrate penetration. The filling technique affected leakage depending on the composite resin and aging regimen.
Polymerization of a dual-cured cement through ceramic: LED curing light vs halogen lamp
Authors: Lopes Lawrence Gonzaga , Franco Eduardo Batista , Name Neto Abrão , Herrera Francyle S. , Kurachi Cristina , Castañeda-Espinosa Juan C. , Lauris José Roberto Pereira
Published: April 2005

The aim of this study was to investigate the influence of light source, LED unit and halogen lamp (HL), on the effectiveness of Enforce dual-cured cement cured under a ceramic disc. Three exposure times (60, 80 and 120 s) were also evaluated. Two experimental groups, in which the polymerization of the dual-cured cement was performed through a ceramic disc, and two control groups, in which the polymerization of the dual-cured cement was performed directly without presence of ceramic disc were subdivided into three subgroups (three different exposure times), with five specimens each: G1A- HL 60s; G1B- HL 80s; G1C- HL 120s; G2A- LED 60s; G2B- LED 80s; G2C- LED 120s; and control groups: G3A- HL 60s; G3B- HL 80s; G3C- HL 120s; G4A- LED 60s; G4B- LED 80s and G4C- LED 120s. Cement was applied in a steel matrix (4mm diameter, 1.2mm thickness). In the experimental groups, a ceramic disc was placed on top. The cement was light-cured through the ceramic by a HL and LED, however, the control groups were cured without the ceramic disc. The specimens were stored in a light-proof container at 37ºC for 24 hours, then Vickers hardness was determined. A four-way ANOVA and Tukey test (p£ 0.05) were performed. All specimens cured by LED for 60s showed inferior values compared with the halogen groups. In general, light-curing by LED for 80s and 120s was comparable to halogen groups (60s and 80s) and their control groups. LED technology can be viable for light-curing through conventional ceramic indirect restorations, when curing time is increased in relation to HL curing time.
Linear density analysis of bone repair in rats using digital direct radiograph
Authors: Bozzo Ricardo de Oliveira , Rocha Rodney Garcia , Haiter Neto Francisco , Paganini Gisela André , Cavalcanti Marcelo Gusmão Paraiso
Published: April 2005

The objective of this study was to assess bone density measurements for analysis of repairing fractures in rats using direct digital radiography under Dexamethasone effects. Thirty (30) young adult male Wistar rats were used, with an average weight of 200g, which were submitted to general anesthetics by ethyl ether. One rib of each animal was fractured by surgical technique. The rats were divided into 2 groups, named Control group - intraperitoneal administration of saline solution 1 hour prior to operation, and Group 1: intraperitoneal administration of Dexamethasone 0.1mg/Kg of weight 1 hour prior to operation, and two postoperative doses every 12 hours. The animals were sacrificed at 2, 3, 4, 7 and 14 days. The parts containing the hemi-thorax with the fractured rib were removed and submitted to direct digital radiography, where the linear density of the two extremities of the fracture were analyzed by the Digora System. The results showed that in the control group and in Group 1 there was a gradual and significant increase in linear density. The method used made it feasible to supply data that were statistically significant in assessing the gain in bone density during the period of time analyzed. In conclusion, direct digital radiograph is useful for bone density analysis for fracture repairing. The dose of Dexamethasone was not sufficient to alter the gain in bone density at the extremities of fracture.
Effect of fluoride and gonadal steroid deficiency on enamel and dentin mineralization of female rats
Authors: Takeshita Eliana Mitsue , Iwama Sayaka , Silva Tatiana Correa da , Dornelles Rita Cássia Menegatti , Delbem Alberto Carlos Botazzo , Sassaki Kikue Takebayashi
Published: April 2005

The aim of this study was to verify the influence of estrogen deficiency on enamel and dentin microhardness of female rats' incisors, as well as the influence of chronic administration of fluoride on that response. Sham or OVX rats drank distilled water or distilled water with 10 ppm fluoride as NaF, for 90 days. The upper incisors were removed and the specimens submitted to microhardness analysis of enamel and dentin. The data were compared by variance analysis and Tukey's test (p<0.05). There was a significant reduction in the mineralization of crown (7.9% and 8.1%) and root (20.4% and 25.0%) in both groups treated with fluoride or distilled water, respectively and an increase (14.2%) in the mineralization of coronal dentin after ovariectomy. Fluoride treatment did not cause reduction in enamel mineralization; however, it reduced dentin mineralization following ovariectomy. It was possible to conclude that estrogens directly or indirectly influence the process of enamel and dentin mineralization of female rats' incisors. The results also indicate that enamel and dentin exhibit different responses to fluoride administration.