bluuebunny/crossref_metadata_2025 huggingface dataset:
Factorial Structure of the EOCL-1 Scale to Assess Executive Functions
Authors: Ramos-Galarza Carlos , Cruz-Cárdenas Jorge , Bolaños-Pasquel Mónica , Acosta-Rodas Pamela
Published: May 2021

The process of assessing executive functions through behavioral observation scales is still under theoretical and empirical construction. This article reports on the analysis of the factorial structure of the EOCL-1 scale that assesses executive functions, as proposed by the theory developed by Luria, which has not been previously considered in this type of evaluation. In this scale, the executive functions taken into account are error correction, internal behavioral and cognition regulatory language, limbic system conscious regulation, decision making, future consideration of consequences of actions, goal-directed behavior, inhibitory control of automatic responses, creation of new behavioral repertoires, and cognitive–behavioral activity verification. A variety of validity and reliability analyses were carried out, with the following results: (a) an adequate internal consistency level of executive functions between α = 0.70 and α = 0.83, (b) significant convergent validity with a scale that assesses frontal deficits between r = −0.07 and r = 0.28, and (c) the scale’s construct validity that proposes a model with an executive central factor comparative fit index (CFI) = 0.93, root mean square error of approximation (RMSEA) = 0.04 (LO.04 and HI.04), standardized root mean square residual (SRMR) = 0.04, and x2(312) = 789.29, p = 0.001. The findings are discussed based on previous literature reports and in terms of the benefits of using a scale to assess the proposed executive functions.
The Relationship Between Confidence and Conformity in a Non-routine Counting Task With Young Children: Dedicated to the Memory of Purificación Rodríguez
Authors: Lago Ma Oliva , Escudero Ana , Dopico Cristina
Published: May 2021

Counting is a complex cognitive process that is paramount to arithmetical development at school. The improvement of counting skills of children depends on their understanding of the logical and conventional rules involved. While the logical rules are mandatory and related to one-to-one correspondence, stable order, and cardinal principles, conventional rules are optional and associated with social customs. This study contributes to unravel the conceptual understanding of counting rules of children. It explores, with a developmental approach, the performance of children on non-routine counting detection tasks, their confidence in their answers (metacognitive monitoring skills), and their ability to change a wrong answer by deferring to the opinion of a unanimous majority who justified or did not justify their claims. Hundred and forty nine children aged from 5 to 8 years were randomized to one of the experimental conditions of the testimony of teachers: with (n = 74) or without justification (n = 75). Participants judged the correctness of different types of counting procedures presented by a computerized detection task, such as (a) pseudoerrors that are correct counts where conventional rules are violated (e.g., first counting six footballs, followed by other six basketballs that were interspersed along the row), and (b) compensation errors that are incorrect counts where logical rules were broken twice (e.g., skipping the third element of the row and then labeling the sixth element with two number words, 5 and 6). Afterwards, children rated their confidence in their detection answer with a 5-point scale. Subsequently, they listened to the testimony of the teachers and showed either conformity or non-conformity. The participants considered both compensation errors and pseudoerrors as incorrect counts in the detection task. The analysis of the confidence of children in their responses suggested that they were not sensitive to their incorrect performance. Finally, children tended to conform more often after hearing a justification of the testimony than after hearing only the testimonies of the teachers. It can be concluded that the age range of the evaluated children failed to recognize the optional nature of conventional counting rules and were unaware of their misconceptions. Nevertheless, the reasoned justifications of the testimony, offered by a unanimous majority, promoted considerable improvement in the tendency of the children to revise those misconceptions.
Whatever Next and Close to My Self—The Transparent Senses and the “Second Skin”: Implications for the Case of Depersonalization
Authors: Ciaunica Anna , Roepstorff Andreas , Fotopoulou Aikaterini Katerina , Petreca Bruna
Published: May 2021

In his paper “Whatever next? Predictive brains, situated agents, and the future of cognitive science,” Andy Clark seminally proposed that the brain's job is to predict whatever information is coming “next” on the basis of prior inputs and experiences. Perception fundamentally subserves survival and self-preservation in biological agents, such as humans. Survival however crucially depends on rapid and accurate information processing of what is happening in the here and now. Hence, the term “next” in Clark's seminal formulation must include not only the temporal dimension (i.e., what is perceivednow) but also the spatial dimension (i.e., what is perceivedhereor next-to-my-body). In this paper, we propose to focus on perceptual experiences that happen “next,” i.e., close-to-my-body. This is because perceptual processing of proximal sensory inputs has a key impact on the organism's survival. Specifically, we focus on tactile experiences mediated by the skin and what we will call the “extended skin” or “second skin,” that is, immediate objects/materials that envelop closely to our skin, namely, clothes. We propose that the skin and tactile experiences are not a mere border separating the self and world. Rather, they simultaneously and inherently distinguishandconnect the bodily self to its environment. Hence, these proximal and pervasive tactile experiences can be viewed as a “transparent bridge” intrinsically relating and facilitating exchanges between the self and the physical and social world. We conclude with potential implications of this observation for the case of Depersonalization Disorder, a condition that makes people feel estranged and detached from their self, body, and the world.
Oncologists' Communication About Uncertain Information in Second Opinion Consultations: A Focused Qualitative Analysis
Authors: van Someren Jamie L. , Lehmann Vicky , Stouthard Jacqueline M. , Stiggelbout Anne M. , Smets Ellen M. A. , Hillen Marij A.
Published: May 2021

Introduction: Uncertainty is omnipresent in cancer care, including the ambiguity of diagnostic tests, efficacy and side effects of treatments, and/or patients' long-term prognosis. During second opinion consultations, uncertainty may be particularly tangible: doubts and uncertainty may drive patients to seek more information and request a second opinion, whereas the second opinion in turn may also affect patients' level of uncertainty. Providers are tasked to clearly discuss all of these uncertainties with patients who may feel overwhelmed by it. The aim of this study was to explore how oncologists communicate about uncertainty during second opinion consultations in medical oncology.

Methods: We performed a secondary qualitative analysis of audio-recorded consultations collected in a prospective study among cancer patients (N = 69) who sought a second opinion in medical oncology. We purposively selected 12 audio-recorded second opinion consultations. Any communication about uncertainty by the oncologist was double coded by two researchers and an inductive analytic approach was chosen to allow for novel insights to arise.

Results: Seven approaches in which oncologists conveyed or addressed uncertainty were identified: (1) specifying the degree of uncertainty, (2) explaining reasons of uncertainty, (3) providing personalized estimates of uncertainty to patients, (4) downplaying or magnifying uncertainty, (5) reducing or counterbalancing uncertainty, and (6) providing support to facilitate patients in coping with uncertainty. Moreover, oncologists varied in their (7) choice of words/language to convey uncertainty (i.e., “I” vs. “we”; level of explicitness).

Discussion: This study identified various approaches of how oncologists communicated uncertain issues during second opinion consultations. These different approaches could affect patients' perception of uncertainty, emotions provoked by it, and possibly even patients' behavior. For example, by minimizing uncertainty, oncologists may (un)consciously steer patients toward specific medical decisions). Future research is needed to examine how these different ways of communicating about uncertainty affect patients. This could also facilitate a discussion about the desirability of certain communication strategies. Eventually, practical and evidence-based guidance needs to be developed for clinicians to optimally inform patients about uncertain issues and support patients in dealing with these.
Sexual Dimorphism in Language, and the Gender Shift Hypothesis of Homosexuality
Authors: Luoto Severi
Published: May 2021

Psychological sex differences have been studied scientifically for more than a century, yet linguists still debate about the existence, magnitude, and causes of such differences in language use. Advances in psychology and cognitive neuroscience have shown the importance of sex and sexual orientation for various psychobehavioural traits, but the extent to which such differences manifest in language use is largely unexplored. Using computerised text analysis (Linguistic Inquiry and Word Count: LIWC 2015), this study found substantial psycholinguistic sexual dimorphism in a large corpus of English-language novels (n = 304) by heterosexual authors. The psycholinguistic sex differences largely aligned with known psychological sex differences, such as empathising–systemising, people–things orientation, and men’s more pronounced spatial cognitive styles and abilities. Furthermore, consistent with predictions from cognitive neuroscience, novels (n = 158) by lesbian authors showed minor signs of psycholinguistic masculinisation, while novels (n = 167) by homosexual men had a female-typical psycholinguistic pattern, supporting the gender shift hypothesis of homosexuality. The findings on this large corpus of 66.9 million words indicate how psychological group differences based on sex and sexual orientation manifest in language use in two centuries of literary art.
Effects of Service Recovery Expectation and Recovery Justice on Customer Citizenship Behavior in the E-Retailing Context
Authors: Zhu Tingting , Liu Beilei , Song Mengmeng , Wu Jinnan
Published: May 2021

Customer citizenship behavior in the online shopping environment is vital to the success of e-retailers. However, it is unclear whether and how service recovery expectation and recovery justice predict customer citizenship behavior in e-retailing settings. Grounded on the expectation confirmation theory and social exchange theory, this study examined the influence of service recovery expectation and recovery justice on customer citizenship behavior with a serial mediation of recovery expectation confirmation and post-recovery satisfaction. A total of 774 samples from e-shoppers with most impressive (N = 401) and most recent (N = 373) service recovery experience were collected to test the hypotheses using structural equation modeling and bootstrapping procedures. This study reveals that service recovery expectation has a negative impact on recovery expectation confirmation, while recovery justice positively affects recovery expectation confirmation, which is further positively correlated with post-recovery satisfaction and customer citizenship behavior. Moreover, recovery expectation confirmation and post-recovery satisfaction play a serial mediation in the relationship between service recovery expectation and recovery justice, and customer citizenship behavior. Our study contributes to the growing body of customer citizenship behavior literature by offering an alternative perspective (i.e., service recovery) to understand what encourage or impede customer citizenship behavior, and expands service recovery literature by combining service recovery expectation and recovery justice into a framework and revealing the expectation–confirmation mechanism through which they influence post-recovery satisfaction in online shopping setting.
The Application of Acceptance Models to Human Resource Information Systems: A Literature Review
Authors: Menant Lou , Gilibert Daniel , Sauvezon Céline
Published: May 2021

Technology acceptance by users has been extensively studied in recent years in various fields such as technologies for learning, e-commerce, and business technologies. This review focuses specifically on Human Resource Information Systems (HRIS) and its acceptance by users. Given their widespread use in organisations, HRIS acceptance has been researched but not synthesised in any way. This article aims to review the effectiveness of the classical TAM and UTAUT models commonly used for new technologies and to identify the variables added to these models to better predict HRIS acceptance by employees. It also highlights the importance of the human-machine-organisation relationship to contribute to the understanding of HRIS acceptance in professional environments. This review confirms the effectiveness of the TAM and UTAUT models and proposes to develop them by (a) variables reffering to technological characteristics (security, system response time, and the data quality implemented in the system), (b) user satisfaction with the system, and (c) organisational variables (expected role of the HR department). The discussion focuses on the retroaction possibilities between the different Human-Machine-Organisation relation levels.
Lexical and Phonetic Influences on the Phonolexical Encoding of Difficult Second-Language Contrasts: Insights From Nonword Rejection
Authors: Llompart Miquel
Published: May 2021

Establishing phonologically robust lexical representations in a second language (L2) is challenging, and even more so for words containing phones in phonological contrasts that are not part of the native language. This study presents a series of additional analyses of lexical decision data assessing the phonolexical encoding of English /ε/ and /æ/ by German learners of English (/æ/ does not exist in German) in order to examine the influence of lexical frequency, phonological neighborhood density and the acoustics of the particular vowels on learners’ ability to reject nonwords differing from real words in the confusable L2 phones only (e.g., *l[æ]mon, *dr[ε]gon). Results showed that both the lexical properties of the target items and the acoustics of the critical vowels affected nonword rejection, albeit differently for items with /æ/ → [ε] and /ε/ → [æ] mispronunciations: For the former, lower lexical frequencies and higher neighborhood densities led to more accurate performance. For the latter, it was only the acoustics of the vowel (i.e., how distinctly [æ]-like the mispronunciation was) that had a significant impact on learners’ accuracy. This suggests that the encoding of /ε/ and /æ/ may not only be asymmetric in that /ε/ is generally more robustly represented in the lexicon than /æ/, as previously reported, but also in the way in which this encoding takes place. Mainly, the encoding of /æ/ appears to be more dependent on the characteristics of the L2 vocabulary and on one’s experience with the L2 than that of its more dominant counterpart (/ε/).
eStroop: Implementation, Standardization, and Systematic Comparison of a New Voice-Key Version of the Traditional Stroop Task
Authors: Brunetti Riccardo , Indraccolo Allegra , Del Gatto Claudia , Farina Benedetto , Imperatori Claudio , Fontana Elena , Penso Jacopo , Ardito Rita B. , Adenzato Mauro
Published: May 2021

The Stroop effect is a well-documented phenomenon, demonstrating both interference and facilitation effects. Many versions of the Stroop task were created, according to the purposes of its applications, varying in numerous aspects. While many versions are developed to investigate the mechanisms of the effect itself, the Stroop effect is also considered a general measure of attention, inhibitory control, and executive functions. In this paper, we implement “eStroop”: a new digital version based on verbal responses, measuring the main processes involved in the traditional effect. eStroop features four categories of stimuli in four different colors: (1) geometrical shapes, (2) neutral words, (3) congruent words, and (4) incongruent words. The results of the administration to 307 University students confirm the Stroop effect and offer baseline data for future research and clinical testing. Direct comparisons with other recent versions of the task are discussed, offering insights into differences and similarities between different task variables.
Large Gatherings? No, Thank You. Devaluation of Crowded Social Scenes During the COVID-19 Pandemic
Authors: Massaccesi Claudia , Chiappini Emilio , Paracampo Riccardo , Korb Sebastian
Published: May 2021

In most European countries, the first wave of the COVID-19 pandemic (spring 2020) led to the imposition of physical distancing rules, resulting in a drastic and sudden reduction of real-life social interactions. Even people not directly affected by the virus itself were impacted in their physical and/or mental health, as well as in their financial security, by governmental lockdown measures. We investigated whether the combination of these events had changed people's appraisal of social scenes by testing 241 participants recruited mainly in Italy, Austria, and Germany in an online, preregistered study conducted about 50 days after the beginning of the COVID-19 outbreak in Europe. Images depicting individuals alone, in small groups (up to four people), and in large groups (more than seven people) were rated in terms of valence, arousal, and perceived physical distance. Pre-pandemic normative ratings were obtained from a validated database (OASIS). Several self-report measures were also taken, and condensed into four factors through factor analysis. All images were rated as more arousing compared to the pre-pandemic period, and the greater the decrease in real-life physical interactions reported by participants, the higher the ratings of arousal. As expected, only images depicting large gatherings of people were rated less positively during, compared to before, the pandemic. These ratings of valence were, however, moderated by a factor that included participants' number of days in isolation, relationship closeness, and perceived COVID-19 threat. Higher scores on this factor were associated with more positive ratings of images of individuals alone and in small groups, suggesting an increased appreciation of safer social situations, such as intimate and small-group contacts. The same factor was inversely related to the perceived physical distance between individuals in images of small and large groups, suggesting an impact of lockdown measures and contagion-related worries on the representation of interpersonal space. These findings point to rapid and compelling psychological and social consequences of the lockdown measures imposed during the COVID-19 pandemic on the perception of social groups. Further studies should assess the long-term impact of such events as typical everyday life is restored.
mHealth-Assisted Detection of Precursors to Relapse in Schizophrenia
Authors: Buck Benjamin , Hallgren Kevin A. , Campbell Andrew T. , Choudhury Tanzeem , Kane John M. , Ben-Zeev Dror
Published: May 2021

Theoretical views and a growing body of empirical evidence suggest that psychiatric relapses in schizophrenia-spectrum disorders (SSDs) have measurable warning signs. However, because they are time- and resource-intensive, existing assessment approaches are not well-suited to detect these warning signs in a timely, scalable fashion. Mobile technologies deploying frequent measurements—i.e., ecological momentary assessment—could be leveraged to detect increases in symptoms that may precede relapses. The present study examined EMA measurements with growth curve models in the 100 days preceding and following 27 relapses (among n = 20 individuals with SSDs) to identify (1) what symptoms changed in the periods gradually preceding, following, and right as relapses occur, (2) how large were these changes, and (3) on what time scale did they occur. Results demonstrated that, on average, participants reported elevations in negative mood (d = 0.34), anxiety (d =0.49), persecutory ideation (d =0.35), and hallucinations (d =0.34) on relapse days relative to their average during the study. These increases emerged gradually on average from significant and steady increases (d = 0.05 per week) in persecutory ideation and hallucinations over the 100-day period preceding relapse. This suggests that brief (i.e., 1–2 item) assessments of psychotic symptoms may detect meaningful signals that precede psychiatric relapses long before they occur. These assessments could increase opportunities for relapse prevention as remote measurement-based care management platforms develop.
The Prevalence of Generalized Anxiety Disorder Among Health Care Workers During the COVID-19 Pandemic: A Systematic Review and Meta-Analysis
Authors: Adibi Amir , Golitaleb Mohamad , Farrahi-Ashtiani Iman , Pirani Davoud , Yousefi Kosar , Jamshidbeigi Yousef , Sahebi Ali
Published: May 2021

Introduction: Health care workers, due to be involved in caring for COVID-19 patients may experience various psychological problems including anxiety disorders. This study aimed to investigate the prevalence of Generalized Anxiety Disorder (GAD) among health care workers during the COVID-19 pandemic by systematic review and meta-analysis.

Methods: The PRISMA guideline was used for conducting this study. Related keywords were searched in credited resources including ISC, Magiran, PubMed, Scopus, Web of Science, Cochrane, ProQuest, Science Direct, Google Scholar, and Embase to find the articles published on the prevalence of GAD among health care workers during the COVID-19 pandemic from the first of January to the end of June 2020. Meta-analysis was conducted by the random effects model.

Results: In this study, 553 articles were initially identified, from which 19 studies were finally included in the meta-analysis. The results showed that the prevalence of GAD in health care workers based on the GAD-7 and GAD-2 instruments were 32.04% (95% CI: 26.89–37.19, I2 = 98.2%, p < 0.001) and 22.62% (95% CI: 9.01–36.24, I2 = 97.7%, p < 0.001). The overall prevalence of GAD was obtained 30.5% (95% CI: 25.58–35.42, I2 = 98.4%, p < 0.001).

Conclusion: This study showed a relatively high GAD prevalence, as one of the fundamental psychological problems, among health care workers during the COVID-19 pandemic. Therefore, health system managers should implement preventive strategies to protect health staff from contracting the virus and monitor them for psychological problems and provide them with supportive measures if necessary.
The Association Between Fear of Coronavirus Disease 2019, Mental Health, and Turnover Intention Among Quarantine Hotel Employees in China
Authors: Teng Yi-Man , Wu Kun-Shan , Xu Dan
Published: May 2021

During the coronavirus disease 2019 (COVID-19) pandemic, quarantine hotel employees face a higher risk of infection while they host quarantine guests from overseas. This is the first research to empirically investigate the psychological effects of operating a quarantine hotel on its employees. The empirical results indicate that heightened fear of COVID-19 leads to adverse mental health issues for quarantine hotel employees and confirm that depression, anxiety, and stress have a significant influence on turnover intention. These findings contribute to the extant knowledge base by uncovering the role of mental health in employee turnover intention. Based on the results, implications are presented for practitioners.
Granulated Rest Frames Outperform Field of View Restrictors on Visual Search Performance
Authors: Cao Zekun , Grandi Jeronimo , Kopper Regis
Published: May 2021

Dynamic field of view (FOV) restrictors have been successfully used to reduce visually induced motion sickness (VIMS) during continuous viewpoint motion control (virtual travel) in virtual reality (VR). This benefit, however, comes at the cost of losing peripheral awareness during provocative motion. Likewise, the use of visual references that are stable in relation to the physical environment, called rest frames (RFs), has also been shown to reduce discomfort during virtual travel tasks in VR. We propose a new RF-based design called Granulated Rest Frames (GRFs) with a soft-edged circular cutout in the center that leverages the rest frames’ benefits without completely blocking the user’s peripheral view. The GRF design is application-agnostic and does not rely on context-specific RFs, such as commonly used cockpits. We report on a within-subjects experiment with 20 participants. The results suggest that, by strategically applying GRFs during a visual search session in VR, we can achieve better item searching efficiency as compared to restricted FOV. The effect of GRFs on reducing VIMS remains to be determined by future work.
Experimental Flash Floods Assessment Through Urban Flood Risk Mitigation (UFRM) Model: The Case Study of Ligurian Coastal Cities
Authors: Quagliolo Carlotta , Comino Elena , Pezzoli Alessandro
Published: May 2021

Cities are vulnerable to extreme weather events, particularly by considering flash flood risk as a result of even more short-duration intensive rainfall. In the context of climate change, compound flooding due to simultaneous storm surges and increased runoff may further exacerbate the risk in coastal cities, and it is expected to be frequent and severe across several European urban areas. Despite this increasing evidence, the spatial knowledge of the hazardous events/vulnerabilities through modelling scenarios at the urban level is quite unexplored. Moreover, flood-prone areas often do not correspond to the traditional flood risk classification based on predicted return-period. The result that huge impacts (human losses and damages) occur everywhere throughout the city. Consequently, this new challenge requires stormwater flooding mitigation strategies to adapt to cities while mainstreaming urban flood resilience. In this paper, we considered the Urban Flood Risk Mitigation model through the employment of the open-source tool—Integrated Evaluation of Ecosystem Services and Trade-off (InVEST)—developed by the Natural Capital Project, integrated into a GIS environment. The model application in the three urban coastal territory of the Liguria Region (Italy) estimated the amount of runoff due to two extreme rainfall events for each watershed considered. These index calculation results help define examples of Natural Water Retention Measures (NWRM) per land-use type as resilient solutions by addressing site-specific runoff reduction. Local sensitivity analysis was finally conducted to comprehend the input parameter's influence of rain variation on the model.
Lifetime Prevalence of Verbal, Physical, and Sexual Abuses in Young Elite Athletics Athletes
Authors: Bermon Stéphane , Adami Paolo Emilio , Dahlström Örjan , Fagher Kristina , Hautala Janna , Ek Anna , Anderson Christer , Jacobsson Jenny , Svedin Carl Göran , Timpka Toomas
Published: May 2021

To examine prevalence of verbal, physical, and sexual abuses in young elite athletes, a cross sectional questionnaire-based survey was conducted during the World Athletics under 20 World Championships. This questionnaire aimed at distinguishing between abuses perpetrated in the context of Athletics from those which were unrelated to Athletics. Four hundred and eighty athletes (52.3%, male) from North America, South America, Europe, Africa, Asia, and Oceania took part in the electronic anonymous survey. Outside Athletics setting, no gender difference was found for the prevalence of verbal, physical, and sexual abuses. However, 45 males (18% of the male population) and 34 females (15% of the female population) athletes reported sexual abuse. Asian athletes reported a slightly higher rate of sexual abuse; three quarters of them being non-touching abuses. Inside Athletics setting, no gender difference was found for the prevalence of verbal, physical, and non-touching sexual abuses. However, 58 males (23%) and 47 females (21%) reported verbal abuses. Thirty-one males (12%) and 20 females (9%) reported physical abuses, whereas 30 males (12%) and 17 females (7%) reported sexual abuses. Physical abuses were slightly more frequent in Asia and in Africa and less frequent in South America. Sexual abuses inside Athletics also differed over regions, and were unexpectedly twice more frequent than expected in Asia and slightly less frequent than expected in Europe. Friends and partners were identified as the more frequent (>50%) abusers outside or inside the Athletics settings, whereas outside Athletics and inside Athletics, coaches were identified as sexual abuse perpetrators in 8 and 25% of cases, respectively. The prevalence of verbal, physical, or sexual abuses is high but consistent with what has been reported in United Kingdom, Norway, Canada, and Sweden at national level in recreational or elite athletes. Sexual abuse, including touching or penetrative abuses, occurred significantly more often in male athletes when compared to female athletes. This finding invites healthcare and social workers, and policymakers to also consider the risk of sexual abuse of young male athletes in Athletics. These results also call for longitudinal studies on young elite athletes.
The Regulation of Staphylococcus aureus-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells
Authors: Cai Mingcheng , Fan Wenqiao , Li Xiaoying , Sun Hanchang , Dai Liuliu , Lei Defang , Dai Ying , Liao Yuhua
Published: May 2021

Mastitis, an inflammatory disease, causes severe economic loss in the dairy industry, which is mainly infected by bacteria. Staphylococcus aureus (S. aureus), the major pathogenic microorganism, derived from lipoteichoic acid (LTA) has been identified to activate inflammatory responses, but the cellular or intercellular regulatory mechanism is unclear. This study mainly focused on the effects of LTA in bovine mammary epithelial cells (Mac-T) and elaborated the regulation of microRNAs (miRNAs). The results showed that LTA enhanced the messenger RNA (mRNA) expression and production of tumor necrosis factor α (TNF-α) and interleukin (IL)-6. Furthermore, LTA could activate Toll-like receptor (TLR)2/MyD88-mediated phosphoinositide 3-kinase (PI3K)/AKT pathway, and TLR2 plays a pivotal role in LTA-induced inflammatory responses. The results of qRT-PCR showed that miRNA levels increased and reached the highest at 3 h and then gradually decreased over time in Mac-T cells. In exosomes, the levels of 11 and three miRNAs were upregulated and downregulated at 24 h, respectively. In addition, miR-23a showed the highest increase in Mac-T cells treated with LTA and targeted PI3K to regulate inflammatory responses. Furthermore, Mac-T cell-derived exosomes were identified to play a cell–cell communication by promoting M1 polarization of bovine macrophages. In summary, our study demonstrated that LTA could activate inflammatory responses via TLR2/MyD88/PI3K/AKT signaling pathway, and miR-23a inhibited it by targeting PI3K. Furthermore, we found that Mac-T cell-derived exosomes might be associated with inflammatory responses by promoting M1 polarization of bovine macrophages.
Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens
Authors: Elazab Sara T. , Elshater Nahla S. , Kishaway Asmaa T. Y. , EI-Emam Huda A.
Published: May 2021

The present study aimed to assess the potential protective effects of cinnamon (Cinnamomum zeylanicum, Cin) and probiotic against CuSO4-induced nephrotoxicity in broiler chickens. One-day-old Cobb chicks were assigned into seven groups (15 birds/group): control group, fed basal diet; Cin group, fed the basal diet mixed with Cin (200 mg/kg); PR group, receiving PR (1 g/4 L water); Cu group, fed the basal diets mixed with CuSO4 (300 mg/kg); Cu + Cin group; Cu + PR group; and Cu + Cin + PR group. All treatments were given daily for 6 weeks. Treatment of Cu-intoxicated chickens with Cin and/or PR reduced (p < 0.05) Cu contents in renal tissues and serum levels of urea, creatinine, and uric acid compared to the Cu group. Moreover, Cin and PR treatment decreased lipid peroxidation and increased antioxidant enzyme activities in chickens’ kidney. Additionally, significant reduction (p < 0.05) in the mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin (IL-2) and Bax, and in cyclooxygenase (COX-II) enzyme expression, and significant elevation (p < 0.05) in mRNA expression of IL-10 and Bcl-2 were observed in kidneys of Cu + Cin, Cu + PR, and Cu + Cin + PR groups compared to Cu group. Conclusively, Cin and/or PR afford considerable renal protection against Cu-induced nephrotoxicity in chickens.
Positive Human Contact and Housing Systems Impact the Responses of Piglets to Various Stressors
Authors: Hayes Megan E. , Hemsworth Lauren M. , Morrison Rebecca S. , Tilbrook Alan J. , Hemsworth Paul H.
Published: May 2021

This experiment studied the effects of lactation housing systems and human interaction on piglets’ responses to routine stressors. Forty litters of piglets were reared in either a standard farrowing crate (FC) or a loose farrowing and lactation pen (LP; PigSAFE pen) and received either routine contact with humans (C) or regular opportunities for positive human contact (+HC; 3 min of patting, stroking and scratching 5 times/week). Behavioural and physiological responses to routine husbandry procedures, weaning, novelty and humans were studied in addition to effects on piglet growth, injuries and survival. Compared to C piglets, +HC piglets vocalised for shorter durations (p = 0.018) during husbandry procedures and showed a lower intensity of escape behaviour during iron injection (p = 0.042) and oral vaccination (p = 0.026) at 3 d of age, capture at 2 wk of age (p < 0.001), and intramuscular vaccination (p = 0.005) at 3 wk of age. +HC piglets at 2 wk of age were faster than C piglets to approach (p = 0.048) and interact (p = 0.042) with a stationary unfamiliar human. Compared to LP piglets, FC piglets showed a lower intensity of escape behaviour during capture and iron administration by a stockperson at 3 d of age (p = 0.043). FC piglets at 2 wk of age were faster than LP piglets to approach (p = 0.005) and interact (p = 0.027) with a novel object and approach (p = 0.009) and interact (p = 0.008) with an unfamiliar human. FC piglets had fewer injuries than LP piglets at 2 wk of age (p = 0.004). +HC pigs had fewer injuries than C pigs after weaning (p = 0.003). After weaning there were more pigs from LP than FC observed to be upright (both stationary, p = 0.002 and walking, p = 0.024), vocalizing (p = 0.004), nosing another pig (p = 0.035) and nosing the pen floor (p = 0.038). There were no significant effects on neutrophil:lymphocyte ratios or plasma cortisol concentrations 1.5 h after weaning. However, 25 h after weaning +HC pigs had higher haptoglobin concentrations than C pigs (p = 0.002), and C/LP pigs had higher cortisol concentrations than +HC/LP and C/FC pigs (p = 0.012). There were no significant effects on piglet growth, the number of piglets born alive or the number stillborn, however there were more piglets weaned from FC than LP (p = 0.035). The results from this experiment raise questions that require further research on the ability of pigs reared in loose pens to cope with stressors such as exposure to humans, novelty, husbandry procedures and weaning. This experiment also provides evidence that regular positive human interaction reduces pigs’ fear of humans and husbandry procedures imposed by stockpeople. More research is required to determine if any of these effects are sustained long-term.
Overview of the Use of Probiotics in Poultry Production
Authors: Krysiak Katarzyna , Konkol Damian , Korczyński Mariusz
Published: May 2021

In recent years, probiotics have become more popular in the world of dietary supplements and feed additives within the poultry industry, acting as antibiotic substitutes. Above all, probiotics are universal feed additives that can be used in conjunction with other additives to promote improved performance and health. Their positive effects can be observed directly in the gastrointestinal tract and indirectly in immunomodulation of the poultry immune system. Nutritional effects seen in flocks given probiotics include increased laying and egg quality, increased daily increments, and improved feed conversion ratio (FCR). There has also been an improvement in the quality of meat. This suggests producers can improve production results through the use of probiotics. In addition to these production effects, bird immunity is improved by allowing the organism to better protect itself against pathogens and stress. The lack of accuracy in the formulation of non-European preparations needs to be further developed due to unknown interactions between probiotic bacteria strains as well as their metabolites. The versatility of probiotics and the fact that the bacteria used in their production are an integral part of animal digestive tracts make them a safe feed additives. Despite restrictions from the European Union, probiotics have potential to improve production and health within the poultry industry and beyond. The following article will review the use of probiotics in poultry production.
The Superiority of Data-Driven Techniques for Estimation of Daily Pan Evaporation
Authors: Kumar Manish , Kumari Anuradha , Kumar Deepak , Al-Ansari Nadhir , Ali Rawshan , Kumar Raushan , Kumar Ambrish , Elbeltagi Ahmed , Kuriqi Alban
Published: May 2021

In the present study, estimating pan evaporation (Epan) was evaluated based on different input parameters: maximum and minimum temperatures, relative humidity, wind speed, and bright sunshine hours. The techniques used for estimating Epan were the artificial neural network (ANN), wavelet-based ANN (WANN), radial function-based support vector machine (SVM-RF), linear function-based SVM (SVM-LF), and multi-linear regression (MLR) models. The proposed models were trained and tested in three different scenarios (Scenario 1, Scenario 2, and Scenario 3) utilizing different percentages of data points. Scenario 1 includes 60%: 40%, Scenario 2 includes 70%: 30%, and Scenario 3 includes 80%: 20% accounting for the training and testing dataset, respectively. The various statistical tools such as Pearson’s correlation coefficient (PCC), root mean square error (RMSE), Nash–Sutcliffe efficiency (NSE), and Willmott Index (WI) were used to evaluate the performance of the models. The graphical representation, such as a line diagram, scatter plot, and the Taylor diagram, were also used to evaluate the proposed model’s performance. The model results showed that the SVM-RF model’s performance is superior to other proposed models in all three scenarios. The most accurate values of PCC, RMSE, NSE, and WI were found to be 0.607, 1.349, 0.183, and 0.749, respectively, for the SVM-RF model during Scenario 1 (60%: 40% training: testing) among all scenarios. This showed that with an increase in the sample set for training, the testing data would show a less accurate modeled result. Thus, the evolved models produce comparatively better outcomes and foster decision-making for water managers and planners.
Comparison of Single-Site Ionospheric Delays Derived from Geometry-Free and Geometry-Based Approaches with an Analysis of Orbit and Clock Error Effects
Authors: Liu Sijing , Zhao Qile , Chen Gang , Hu Zhigang , Chao Nengfang
Published: May 2021

The reparameterization of the geometry-free and geometry-based approaches to derive single-site ionospheric delays using Global Navigation Satellite System (GNSS) measurements is described. Kalman filtering is used to compute the geometry-free and geometry-based ionospheric delays in a forward computation procedure, aiming for a real-time application case. The numerical similarity and differences between the geometry-free and geometry-based ionospheric delays are assessed in terms of both formal and experimental errors (precision). The differences between geometry-free and geometry-based ionospheric delays are derived using two types of precise orbit and clock products. The effects of the precise orbit and clock residual errors are analyzed. The correlation coefficients between the L1 and L2 wide-lane ambiguities with the ionospheric delay are derived and analyzed. It is discovered that the geometry-based ionospheric delay is negatively correlated with geometry-based wide-lane ambiguities, while the geometry-free ionospheric delay and wide-lane ambiguities are much less correlated. A simulation analysis indicates that the impacts on geometry-based ionospheric delay estimates are partly coincided with the actual time-variant errors of the used orbit and clock in the line-of-sight direction.
Sign-Singularity Analysis of Field-Aligned Currents in the Ionosphere
Authors: Consolini Giuseppe , De Michelis Paola , Coco Igino , Alberti Tommaso , Marcucci Maria Federica , Giannattasio Fabio , Tozzi Roberta
Published: May 2021

Field-aligned currents (FACs) flowing in the auroral ionosphere are a complex system of upward and downward currents, which play a fundamental role in the magnetosphere–ionosphere coupling and in the ionospheric heating. Here, using data from the ESA-Swarm multi-satellite mission, we studied the complex structure of FACs by investigating sign-singularity scaling features for two different conditions of a high-latitude substorm activity level as monitored by the AE index. The results clearly showed the sign-singular character of FACs supporting the complex and filamentary nature of these currents. Furthermore, we found evidence of the occurrence of a topological change of these current systems, which was accompanied by a change of the scaling features at spatial scales larger than 30 km. This change was interpreted in terms of a sort of symmetry-breaking phenomenon due to a dynamical topological transition of the FAC structure as a consequence of FACs and substorm current wedge intensification during substorms.
Dopamine System, NMDA Receptor and EGF Family Expressions in Brain Structures of Bl6 and 129Sv Strains Displaying Different Behavioral Adaptation
Authors: Varul Jane , Eskla Kattri-Liis , Piirsalu Maria , Innos Jürgen , Philips Mari-Anne , Visnapuu Tanel , Plaas Mario , Vasar Eero
Published: May 2021

C57BL/6NTac (Bl6) and 129S6/SvEvTac (129Sv) mice display different coping strategies in stressful conditions. Our aim was to evaluate biomarkers related to different adaptation strategies in the brain of male 129Sv and Bl6 mice. We focused on signaling pathways related to the dopamine (DA) system, N-methyl-D-aspartate (NMDA) receptor and epidermal growth factor (EGF) family, shown as the key players in behavioral adaptation. Mice from Bl6 and 129Sv lines were divided into either home cage controls (HCC group) or exposed to repeated motility testing and treated with saline for 11 days (RMT group). Distinct stress responses were reflected in severe body weight loss in 129Sv and the increased exploratory behavior in Bl6 mice. Besides that, amphetamine caused significantly stronger motor stimulation in Bl6. Together with the results from gene expression (particularly Maob), this study supports higher baseline activity of DA system in Bl6. Interestingly, the adaptation is reflected with opposite changes of DA markers in dorsal and ventral striatum. In forebrain, stress increased the gene expressions of Egf-Erbb1 and Nrg1/Nrg2-Erbb4 pathways more clearly in 129Sv, whereas the corresponding proteins were significantly elevated in Bl6. We suggest that not only inhibited activity of the DA system, but also reduced activity of EGF family and NMDA receptor signaling underlies higher susceptibility to stress in 129Sv. Altogether, this study underlines the better suitability of 129Sv for modelling neuropsychiatric disorders than Bl6.
Brain Structure and Functional Connectivity Correlate with Psychosocial Development in Contemplative Practitioners and Controls
Authors: Singleton Omar , Newlon Max , Fossas Andres , Sharma Beena , Cook-Greuter Susanne R. , Lazar Sara W.
Published: May 2021

Jane Loevinger’s theory of adult development, termed ego development (1966) and more recently maturity development, provides a useful framework for understanding the development of the self throughout the lifespan. However, few studies have investigated its neural correlates. In the present study, we use structural and functional magnetic resonance imaging (MRI) to investigate the neural correlates of maturity development in contemplative practitioners and controls. Since traits possessed by individuals with higher levels of maturity development are similar to those attributed to individuals at advanced stages of contemplative practice, we chose to investigate levels of maturity development in meditation practitioners as well as matched controls. We used the Maturity Assessment Profile (MAP) to measure maturity development in a mixed sample of participants composed of 14 long-term meditators, 16 long-term yoga practitioners, and 16 demographically matched controls. We investigated the relationship between contemplative practice and maturity development with behavioral, seed-based resting state functional connectivity, and cortical thickness analyses. The results of this study indicate that contemplative practitioners possess higher maturity development compared to a matched control group, and in addition, maturity development correlates with cortical thickness in the posterior cingulate. Furthermore, we identify a brain network implicated in theory of mind, narrative, and self-referential processing, comprising the posterior cingulate cortex, dorsomedial prefrontal cortex, temporoparietal junction, and inferior frontal cortex, as a primary neural correlate.
NR4A1 Ligands as Potent Inhibitors of Breast Cancer Cell and Tumor Growth
Authors: Karki Keshav , Mohankumar Kumaravel , Schoeller Abigail , Martin Gregory , Shrestha Rupesh , Safe Stephen
Published: May 2021

Nuclear receptor 4A1 (NR4A1, Nur77, TR3) is more highly expressed in breast and solid tumors compared to non-tumor tissues and is a pro-oncogenic factor in solid tumor-derived cancers. NR4A1 regulates cancer cell growth, survival, migration, and invasion, and bis-indole-derived compounds (CDIMs) that bind NR4A1 act as antagonists and inhibit tumor growth. Preliminary structure-binding studies identified 1,1-bis(3′-indolyl)-1-(3,5-disubstitutedphenyl)methane analogs as NR4A1 ligands with low KD values; we further investigated the anticancer activity of the four most active analogs (KD’s ≤ 3.1 µM) in breast cancer cells and in athymic mouse xenograft models. The treatment of MDA-MB-231 and SKBR3 breast cancer cells with the 3-bromo-5-methoxy, 3-chloro-5-trifluoromethoxy, 3-chloro-5-trifluoromethyl, and 3-bromo-5-trifluoromethoxy phenyl-substituted analogs decreased cell growth and the expression of epidermal of growth factor receptor (EGFR), hepatocyte growth factor receptor (cMET), and PD-L1 as well as inhibited mTOR phosphorylation. In addition, all four compounds inhibited tumor growth in athymic nude mice bearing MDA-MB-231 cells (orthotopic) at a dose of 1 mg/kg/d, which was not accompanied by changes in body weight. These 3,5-disubstituted analogs were the most potent CDIM/NR4A1 ligands reported and are being further developed for clinical applications.