bluuebunny/crossref_metadata_2025 huggingface dataset:
Bifidobacterium breve Alleviates DSS-Induced Colitis in Mice by Maintaining the Mucosal and Epithelial Barriers and Modulating Gut Microbes
Authors: Niu Meng-Meng , Guo Huan-Xin , Cai Jun-Wu , Meng Xiang-Chen
Published: September 2022

This study was designed to explore the different intestinal barrier repair mechanisms of Bifidobacterium breve (B. breve) H4-2 and H9-3 with different exopolysaccharide (EPS) production in mice with colitis. The lipopolysaccharide (LPS)-induced IEC-6 cell inflammation model and dextran sulphate sodium (DSS)-induced mice colitis model were used. Histopathological changes, epithelial barrier integrity, short-chain fatty acid (SCFA) content, cytokine levels, NF-κB expression level, and intestinal flora were analyzed to evaluate the role of B. breve in alleviating colitis. Cell experiments indicated that both B. breve strains could regulate cytokine levels. In vivo experiments confirmed that oral administration of B. breve H4-2 and B. breve H9-3 significantly increased the expression of mucin, occludin, claudin-1, ZO-1, decreased the levels of IL-6, TNF-α, IL-1β and increased IL-10. Both strains of B. breve also inhibited the expression of the NF-κB signaling pathway. Moreover, B. breve H4-2 and H9-3 intervention significantly increased the levels of SCFAs, reduced the abundance of Proteobacteria and Bacteroidea, and increased the abundance of Muribaculaceae. These results demonstrate that EPS-producing B. breve strains H4-2 and H9-3 can regulate the physical, immune, and microbial barrier to repair the intestinal damage caused by DSS in mice. Of the two strains, H4-2 had a higher EPS output and was more effective at repair than H9-3. These results will provide insights useful for clinical applications and the development of probiotic products for the treatment of colitis.
Maternal Passive Smoking, Vitamin D Deficiency and Risk of Spontaneous Abortion
Authors: Lin Shiqi , Li Jiajia , Zhang Yuan , Song Xinming , Chen Gong , Pei Lijun
Published: September 2022

Background: Maternal passive smoking and vitamin D deficiency might elevate risk of spontaneous abortion. The study aimed to investigate the association of co-exposure to passive smoking and vitamin D deficiency with the risk of spontaneous abortion. Methods: A population-based case-control study was performed among non-smoking women in Henan Province, China, with 293 spontaneous abortion cases and 496 liveborn controls with term, normal birthweight. Results: Compared to women without exposure to passive smoking nor vitamin D deficiency, women with deficient vitamin D alone and women with exposure to passive smoking alone had increased risk of spontaneous abortion (OR = 1.76, 95%CI: 1.08~2.89; OR = 1.73, 95%CI: 1.11~2.69, respectively). The risk of spontaneous abortion was even higher for those with co-exposure to passive smoking and vitamin D deficiency (OR = 2.50, 95%CI: 1.63~3.84). A dose-response relationship was found of an incremental risk of spontaneous abortion with rising numbers of exposures to passive smoking and vitamin D deficiency (p < 0.001). Conclusion: Co-exposure to passive smoking and vitamin D deficiency was associated with an elevated risk of spontaneous abortion, and the risk of spontaneous abortion rose with rising numbers of exposures. Intervention programs need to specifically target the vulnerable groups of pregnant women with both malnutrition and unfavorable environmental exposure.
Surface Layer Protein Pattern of Levilactobacillus brevis Strains Investigated by Proteomics
Authors: Mazzeo Maria Fiorella , Reale Anna , Di Renzo Tiziana , Siciliano Rosa Anna
Published: September 2022

The outermost constituent of many bacterial cells is represented by an S-layer, i.e., a semiporous lattice-like layer composed of self-assembling protein subunits called S-layer proteins (Slps). These proteins are involved in several processes, such as protecting against environmental stresses, mediating bacterial adhesion to host cells, and modulating gut immune response. Slps may also act as a scaffold for the external display of additional cell surface proteins also named S-layer associated proteins (SLAPs). Levilactobacillus brevis is an S-layer forming lactic acid bacterium present in many different environments, such as sourdough, milk, cheese, and the intestinal tract of humans and animals. This microorganism exhibits probiotic features including the inhibition of bacterial infection and the improvement of human immune function. The potential role of Slps in its probiotic and biotechnological features was documented. A shotgun proteomic approach was applied to identify in a single experiment both the Slps and the SLAPs pattern of five different L. brevis strains isolated from traditional sourdoughs of the Southern Italian region. This study reveals that these closely related strains expressed a specific pattern of surface proteins, possibly affecting their peculiar properties.
Ultra-Processed Foods as Ingredients of Culinary Recipes Shared on Popular Brazilian YouTube Cooking Channels
Authors: Camargo Anice Milbratz de , Botelho Alyne Michelle , Camargo Állan Milbratz de , Dean Moira , Fiates Giovanna Medeiros Rataichesck
Published: September 2022

Social media platforms are readily accessible sources of information about cooking, an activity deemed crucial for the improvement of a population’s diet. Previous research focused on the healthiness of the content shared on websites and blogs, but not on social media such as YouTube®. This paper analysed the healthiness of 823 culinary recipes retrieved from 755 videos shared during a six-month period on ten popular Brazilian YouTube® cooking channels. Recipes were categorized by type of preparation. To assess recipes’ healthiness, ingredients were classified according to the extension and purpose of industrial processing, in order to identify the use of ultra-processed foods. Additionally, a validated framework developed from criteria established in both editions of the Dietary Guidelines for the Brazilian Population was employed. Recipes for cakes and baked goods, puddings, snacks and homemade fast foods, which were among the most frequently posted, contained the lowest proportion of unprocessed/minimally processed ingredients and the highest proportion of ultra-processed ingredients. Recipes containing whole cereals, fruits, legumes, nuts, and seeds were scarce. Results indicate that users should be critical about the quality of recipes shared on YouTube® videos, also indicating a need for strategies aimed at informing individuals on how to choose healthier recipes or adapt them to become healthier.
The Impact of Dietary Consumption of Palm Oil and Olive Oil on Lipid Profile and Hepatocyte Injury in Hypercholesterolemic Rats
Authors: Albrahim Tarfa , Alotaibi Maram H. M. , Altamimi Norah M. M. , Albariqi Atheer M. A. , Alqarni Lamees A. O. , Alassaf Sara N. A. , Aloudah Hisham S. , Alahmed Mohammed , Almnaizel Ahmad T. , Aldraihem Maha R. , Alonazi Mona
Published: September 2022

A metabolic disease called hypercholesterolemia is connected to both oxidative damage and inflammation. The goal of the current investigation was to determine if olive oil and palm oil could prevent hypercholesterolemia-induced oxidative stress in the liver of rats fed a high-cholesterol diet (HCD). The experimental mice were given HCD for three months while also receiving 0.5 mL/kg of either palm or olive oil. Serum triglycerides, total cholesterol, LDL cholesterol, vLDL cholesterol, and the atherogenic index all significantly increased in HCD-fed rats, while HDL cholesterol significantly dropped. Additionally, HCD caused a notable rise in proinflammatory cytokines and serum transaminases in liver tissue. Additionally, HCD significantly increased the production of nitric oxide and lipid peroxidation in the liver while decreasing antioxidant enzymes. Treatment with palm and olive oils dramatically reduced the levels of pro-inflammatory cytokines and lipid peroxidation, improved antioxidant defenses, and considerably improved liver function indicators. Additionally, the examined oils dramatically decreased the expression of fatty acid synthase (FAS) in the liver of rats receiving HCD. In conclusion, HCD-fed rats exhibit significant antihyperlipidemic and cholesterol-lowering benefits from palm and olive oils. The improved antioxidant defenses, lower inflammation and lipid peroxidation, and altered hepatic FAS mRNA expression were the main mechanisms by which palm and olive oils produced their advantageous effects.
Plasma Pharmacokinetics and Tissue Distribution of Doxorubicin in Rats following Treatment with Astragali Radix
Authors: Huang Yin , Yang Fang , Guo Linling , Xu Yan , Yu Xiaxia , Zhang Zunjian , Zhang Yuxin
Published: September 2022

Doxorubicin (DOX) is an essential component in chemotherapy, and Astragali Radix (AR) is a widely used tonic herbal medicine. The combination of DOX and AR offers widespread, well-documented advantages in treating cancer, e.g., reducing the risk of adverse effects. This study mainly aims to uncover the impact of AR on DOX disposition in vivo. Rats received a single intravenous dose of 5 mg/kg DOX following a single-dose co-treatment or multiple-dose pre-treatment of AR (10 g/kg × 1 or × 10). The concentrations of DOX in rat plasma and six tissues, including heart, liver, lung, kidney, spleen, and skeletal muscle, were determined by a fully validated LC-MS/MS method. A network-based approach was further employed to quantify the relationships between enzymes that metabolize and transport DOX and the targets of nine representative AR components in the human protein–protein interactome. We found that short-term (≤10 d) AR administration was ineffective in changing the plasma pharmacokinetics of DOX in terms of the area under the concentration–time curve (AUC, 1303.35 ± 271.74 μg/L*h versus 1208.74 ± 145.35 μg/L*h, p > 0.46), peak concentrations (Cmax, 1351.21 ± 364.86 μg/L versus 1411.01 ± 368.38 μg/L, p > 0.78), and half-life (t1/2, 31.79 ± 5.12 h versus 32.05 ± 6.95 h, p > 0.94), etc. Compared to the isotype control group, DOX concentrations in six tissues slightly decreased under AR pre-administration but only showed statistical significance (p < 0.05) in the liver. Using network analysis, we showed that five of the nine representative AR components were not localized to the vicinity of the DOX disposition-associated module. These findings suggest that AR may mitigate DOX-induced toxicity by affecting drug targets rather than drug disposition.
Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations
Authors: Singh Ruby , Birru Bhaskar , Veit Joachim G. S. , Arrigali Elizabeth M. , Serban Monica A.
Published: September 2022

Hearing loss and balance disorders are highly common disorders, and the development of effective oto-therapeutics remains an area of intense research. Drug development and screening in the hearing research field heavily rely on the use of preclinical models with often ambiguous translational relevance. This often leads to failed advancement in the market of effective therapeutics. In this context, especially for inner ear-specific pathologies, the availability of an in vitro, physiologically relevant, round window membrane (RWM) model could enable rapid, high-throughput screening of potential topical drugs for inner ear and cochlear dysfunctions and could help accelerate the advancement to clinic and market of more viable drug candidates. In this study, we report the development and evaluation of an in vitro model that mimics the native RWM tissue morphology and microenvironment as shown via immunostaining and histological analyses. The developed three-dimensional (3D) in vitro model was additionally assessed for barrier integrity by transepithelial electrical resistance, and the permeability of lipophilic and hydrophilic drugs was determined. Our collective findings suggest that this in vitro model could serve as a tool for rapid development and screening of topically deliverable oto-therapeutics.
Phosphatidylcholine-Based Nanoemulsions for Paclitaxel and a P-Glycoprotein Inhibitor Delivery and Breast Cancer Intraductal Treatment
Authors: Salata Giovanna Cassone , Lopes Luciana B.
Published: September 2022

In this study, incorporation of the cytotoxic agent paclitaxel and the P-glycoprotein inhibitor elacridar in hyaluronic acid (HA)-modified nanoemulsions was studied for intraductal delivery and breast cancer localized treatment. To improve cytotoxicity, we investigated the incorporation of perillyl alcohol or tributyrin as components of the nanoemulsion oil phase. The nanoemulsions presented size <180 nm and negative zeta potential. Both tributyrin and perillyl alcohol increased nanoemulsion cytotoxicity in MCF-7 cells, but not in MDA-MB-231. However, perillyl alcohol reduced nanoemulsion stability in the presence of the drugs. Concomitant incorporation of paclitaxel and elacridar in HA- and tributyrin-containing nanoemulsions (PE-NETri) increased cytotoxicity and reduced IC50 by 1.6 to 3-fold in MCF-7 and MDA-MB-231 cells compared to the nanoemulsion containing only paclitaxel (P-NE). This nanoemulsion also produced a 3.3-fold reduction in the viability of MDA-MB-231 spheroids. Elacridar incorporated in the nanoemulsion was capable of inhibiting P-glycoprotein in membranes. In vivo intraductal administration of the NE containing HA resulted in a three-fold higher retention of a fluorescent marker compared to a solution or nanoemulsion without HA, demonstrating the importance of HA. The nanoemulsion produced no histological changes in the mammary tissue. These results support the potential applicability of the nanoemulsion for local breast cancer management.
Nanoscale Delivery Systems of Lutein: An Updated Review from a Pharmaceutical Perspective
Authors: Algan Aslihan Hilal , Gungor-Ak Ayca , Karatas Aysegul
Published: September 2022

Carotenoids are natural lipid-soluble pigments that produce yellow to red colors in plants as well as providing bright coloration in vegetables and fruits. Lutein belongs to the xanthophyll subgroup of the carotenoid family, which plays an essential role in photosynthesis and photoprotection in nature. In the human body, lutein, together with its isomer zeaxanthin and its metabolite meso-zeaxanthin, accumulates in the macula of the eye retina, which is responsible for central, high-resolution, and color vision. As a bioactive phytochemical, lutein has essential physiological functions, providing photoprotection against damaging blue light, along with the neutralization of oxidants and the preservation of the structural and functional integrity of cellular membranes. As a potent antioxidant and anti-inflammatory agent, lutein unfortunately has a low bioavailability because of its lipophilicity and a low stability as a result of its conjugated double bonds. In order to enhance lutein stability and bioavailability and achieve its controlled delivery to a target, nanoscale delivery systems, which have great potential for the delivery of bioactive compounds, are starting to be employed. The current review highlights the advantages and innovations associated with incorporating lutein within promising nanoscale delivery systems, such as liposomes, nanoemulsions, polymer nanoparticles, and polymer–lipid hybrid nanoparticles, as well as their unique physiochemical properties.
Biosynthesis of Silver Nanoparticles Using Seasonal Samples of Sonoran Desert Propolis: Evaluation of Its Antibacterial Activity against Clinical Isolates of Multi-Drug Resistant Bacteria
Authors: Mendez-Pfeiffer Pablo , Ballesteros-Monrreal Manuel G. , Gaona-Ochoa Jesus , Juarez Josue , Gastelum-Cabrera Marisol , Montaño-Leyva Beatriz , Arenas-Hernández Margarita , Caporal-Hernandez Liliana , Ortega-García Jesús , Barrios-Villa Edwin , Velazquez Carlos , Valencia Dora
Published: September 2022

Multi-drug resistant (MDR) bacteria have gained importance as a health problem worldwide, and novel antibacterial agents are needed to combat them. Silver nanoparticles (AgNPs) have been studied as a potent antimicrobial agent, capable of countering MDR bacteria; nevertheless, their conventional synthesis methods can produce cytotoxicity and environmental hazards. Biosynthesis of silver nanoparticles has emerged as an alternative to reduce the cytotoxic and environmental problems derived from their chemical synthesis, using natural products as a reducing and stabilizing agent. Sonoran Desert propolis (SP) is a poplar-type propolis rich in polyphenolic compounds with remarkable biological activities, such as being antioxidant, antiproliferative, and antimicrobial, and is a suitable candidate for synthesis of AgNPs. In this study, we synthesized AgNPs using SP methanolic extract (SP-AgNPs) and evaluated the reduction capacity of their seasonal samples and main chemical constituents. Their cytotoxicity against mammalian cell lines and antibacterial activity against multi-drug resistant bacteria were assessed. Quercetin and galangin showed the best-reduction capacity for synthesizing AgNPs, as well as the seasonal sample from winter (SPw-AgNPs). The SPw-AgNPs had a mean size of around 16.5 ± 5.3 nm, were stable in different culture media, and the presence of propolis constituents was confirmed by FT-IR and HPLC assays. The SPw-AgNPs were non-cytotoxic to ARPE-19 and HeLa cell lines and presented remarkable antibacterial and antibiofilm activity against multi-drug resistant clinical isolates, with E. coli 34 and ATCC 25922 being the most susceptible (MBC = 25 μg/mL), followed by E. coli 2, 29, 37 and PNG (MBC = 50 μg/mL), and finally E. coli 37 and S. aureus ATCC 25923 (MBC = 100 μg/mL). These results demonstrated the efficacy of SP as a reducing and stabilizing agent for synthesis of AgNPs and their capacity as an antibacterial agent.
Design of Hybrid Polymer Nanofiber/Collagen Patches Releasing IGF and HGF to Promote Cardiac Regeneration
Authors: Kerignard Eloise , Bethry Audrey , Falcoz Chloé , Nottelet Benjamin , Pinese Coline
Published: September 2022

Cardiovascular diseases are the leading cause of death globally. Myocardial infarction in particular leads to a high rate of mortality, and in the case of survival, to a loss of myocardial functionality due to post-infarction necrosis. This functionality can be restored by cell therapy or biomaterial implantation, and the need for a rapid regeneration has led to the development of bioactive patches, in particular through the incorporation of growth factors (GF). In this work, we designed hybrid patches composed of polymer nanofibers loaded with HGF and IGF and associated with a collagen membrane. Among the different copolymers studied, the polymers and their porogens PLA-Pluronic-PLA + PEG and PCL + Pluronic were selected to encapsulate HGF and IGF. While 89 and 92% of IGF were released in 2 days, HGF was released up to 58% and 50% in 35 days from PLA-Pluronic-PLA + PEG and PCL + Pluronic nanofibers, respectively. We also compared two ways of association for the loaded nanofibers and the collagen membrane, namely a direct deposition of the nanofibers on a moisturized collagen membrane (wet association), or entrapment between collagen layers (sandwich association). The interfacial cohesion and the degradation properties of the patches were evaluated. We also show that the sandwich association decreases the burst release of HGF while increasing the release efficiency. Finally, we show that the patches are cytocompatible and that the presence of collagen and IGF promotes the proliferation of C2C12 myoblast cells for 11 days. Taken together, these results show that these hybrid patches are of interest for cardiac muscle regeneration.
Gut Microbiota and Bile Acids Mediate the Clinical Benefits of YH1 in Male Patients with Type 2 Diabetes Mellitus: A Pilot Observational Study
Authors: Huang Yueh-Hsiang , Wu Yi-Hong , Tang Hsiang-Yu , Chen Szu-Tah , Wang Chih-Ching , Ho Wan-Jing , Lin Yi-Hsuan , Liu Geng-Hao , Lin Pei-Yeh , Lo Chi-Jen , Yeh Yuan-Ming , Cheng Mei-Ling
Published: September 2022

Our previous clinical trial showed that a novel concentrated herbal extract formula, YH1 (Rhizoma coptidis and Shen-Ling-Bai-Zhu-San), improved blood glucose and lipid control. This pilot observational study investigated whether YH1 affects microbiota, plasma, and fecal bile acid (BA) compositions in ten untreated male patients with type 2 diabetes (T2D), hyperlipidemia, and a body mass index ≥ 23 kg/m2. Stool and plasma samples were collected for microbiome, BA, and biochemical analyses before and after 4 weeks of YH1 therapy. As previous studies found, the glycated albumin, 2-h postprandial glucose, triglycerides, total cholesterol, and low-density lipoprotein cholesterol levels were significantly improved after YH1 treatment. Gut microbiota revealed an increased abundance of the short-chain fatty acid-producing bacteria Anaerostipes and Escherichia/Shigella. Furthermore, YH1 inhibited specific phylotypes of bile salt hydrolase-expressing bacteria, including Parabacteroides, Bifidobacterium, and Bacteroides caccae. Stool tauro-conjugated BA levels increased after YH1 treatment. Plasma total BAs and 7α-hydroxy-4-cholesten-3-one (C4), a BA synthesis indicator, were elevated. The reduced deconjugation of BAs and increased plasma conjugated BAs, especially tauro-conjugated BAs, led to a decreased glyco- to tauro-conjugated BA ratio and reduced unconjugated secondary BAs. These results suggest that YH1 ameliorates T2D and hyperlipidemia by modulating microbiota constituents that alter fecal and plasma BA compositions and promote liver cholesterol-to-BA conversion and glucose homeostasis.
Direct Potential Modulation of Neurogenic Differentiation Markers by Granulocyte-Colony Stimulating Factor (G-CSF) in the Rodent Brain
Authors: Kozole Judith , Heydn Rosmarie , Wirkert Eva , Küspert Sabrina , Aigner Ludwig , Bruun Tim-Henrik , Bogdahn Ulrich , Peters Sebastian , Johannesen Siw
Published: September 2022

The hematopoietic granulocyte-colony stimulating growth factor (G-CSF, filgrastim) is an approved drug in hematology and oncology. Filgrastim’s potential in neurodegenerative disorders is gaining increasingly more attention, as preclinical and early clinical studies suggest it could be a promising treatment option. G-CSF has had a tremendous record as a safe drug for more than three decades; however, its effects upon the central nervous system (CNS) are still not fully understood. In contrast to conceptual long-term clinical application with lower dosing, our present pilot study intends to give a first insight into the molecular effects of a single subcutaneous (s.c.) high-dose G-CSF application upon different regions of the rodent brain. We analyzed mRNA—and in some instances—protein data of neurogenic and non-neurogenic differentiation markers in different regions of rat brains five days after G-CSF (1.3 mg/kg) or physiological saline. We found a continuous downregulation of several markers in most brain regions. Remarkably, cerebellum and hypothalamus showed an upregulation of different markers. In conclusion, our study reveals minor suppressive or stimulatory effects of a single exceptional high G-CSF dose upon neurogenic and non-neurogenic differentiation markers in relevant brain regions, excluding unregulated responses or unexpected patterns of marker expression.
Plasmid DNA for Therapeutic Applications in Cancer
Authors: Martínez-Puente David Hernán , Pérez-Trujillo José Juan , Zavala-Flores Laura Mireya , García-García Aracely , Villanueva-Olivo Arnulfo , Rodríguez-Rocha Humberto , Valdés Jesús , Saucedo-Cárdenas Odila , Montes de Oca-Luna Roberto , Loera-Arias María de Jesús
Published: September 2022

Recently, the interest in using nucleic acids for therapeutic applications has been increasing. DNA molecules can be manipulated to express a gene of interest for gene therapy applications or vaccine development. Plasmid DNA can be developed to treat different diseases, such as infections and cancer. In most cancers, the immune system is limited or suppressed, allowing cancer cells to grow. DNA vaccination has demonstrated its capacity to stimulate the immune system to fight against cancer cells. Furthermore, plasmids for cancer gene therapy can direct the expression of proteins with different functions, such as enzymes, toxins, and cytotoxic or proapoptotic proteins, to directly kill cancer cells. The progress and promising results reported in animal models in recent years have led to interesting clinical results. These DNA strategies are expected to be approved for cancer treatment in the near future. This review discusses the main strategies, challenges, and future perspectives of using plasmid DNA for cancer treatment.
Dexamethasone and Doxycycline Doped Nanoparticles Increase the Differentiation Potential of Human Bone Marrow Stem Cells
Authors: Toledano-Osorio Manuel , López-García Sergio , Osorio Raquel , Toledano Manuel , García-Bernal David , Sánchez-Bautista Sonia , Rodríguez-Lozano Francisco Javier
Published: September 2022

Non-resorbable polymeric nanoparticles (NPs) are proposed as an adjunctive treatment for bone regenerative strategies. The present in vitro investigation aimed to evaluate the effect of the different prototypes of bioactive NPs loaded with zinc (Zn-NPs), doxycycline (Dox-NPs) or dexamethasone (Dex-NPs) on the viability, morphology, migration, adhesion, osteoblastic differentiation, and mineralization potential of human bone marrow stem cells (hBMMSCs). Cell viability, proliferation, and differentiation were assessed using a resaruzin-based assay, cell cycle analysis, cell migration evaluation, cell cytoskeleton staining analysis, Alizarin Red S staining, and expression of the osteogenic-related genes by a real-time quantitative polymerase chain reaction (RT-qPCR). One-Way ANOVA and Tukey’s test were employed. The resazurin assay showed adequate cell viability considering all concentrations and types of NPs at 24, 48, and 72 h of culture. The cell cycle analysis revealed a regular cell cycle profile at 0.1, 1, and 10 µg/mL, whereas 100 µg/mL produced an arrest of cells in the S phase. Cells cultured with 0.1 and 1 µg/mL NP concentrations showed a similar migration capacity to the untreated group. After 21 days, mineralization was increased by all the NPs prototypes. Dox-NPs and Dex-NPs produced a generalized up-regulation of the osteogenic-related genes. Dex-NPs and Dox-NPs exhibited excellent osteogenic potential and promoted hBMMSC differentiation. Future investigations, both in vitro and in vivo, are required to confirm the suitability of these NPs for their clinical application.
Combining Essential Oils with Each Other and with Clotrimazole Prevents the Formation of Candida Biofilms and Eradicates Mature Biofilms
Authors: Silva Rafael Alves da , Silva Nagela Bernadelli Sousa , Martins Carlos Henrique Gomes , Pires Regina Helena , Röder Denise Von Dolinger de Brito , Pedroso Reginaldo dos Santos
Published: September 2022

Fungal infections by Candida spp. are opportunistic and most often occur in individuals with some predisposing factor. Essential oils (EO) have anti-Candida potential, being a therapeutic alternative to be explored, especially for superficial and mucosal candidiasis. The objective was to analyze the synergistic potential between the EO of Citrus limon, Cupressus sempervirens, Litsea cubeba and Melaleuca alternifolia, and each of them with clotrimazole, to inhibit in vitro the formation and eradication of Candida spp. biofilms. Added to this, the survival of Caenorhabditis elegans was evaluated after exposure to EO, clotrimazole and their synergistic combinations. Anti-Candida activity was determined by microdilution for the substances alone and in EO–EO and EO–clotrimazole combinations. The combinations were performed by the checkerboard method, and the reduction in the metabolic activity of biofilms was determined by the viability of MTT/menadione. C. elegans larvae survival was evaluated after 24 h of exposure to EO, clotrimazole and synergistic combinations. The minimum inhibitory concentration (MIC) of EO ranged from 500 to >4000 µg/mL. The lowest MIC (500 µg/mL) was for C. sempervirens and L. cubeba on a C. krusei isolate; for clotrimazole, the MIC ranged from 0.015 to 0.5 µg/mL. Biofilm inhibition and eradication both ranged from 1000 to >4000 µg/mL. The lethal concentration (LC50) of C. limon, L. cubeba and M. alternifolia was 2000 µg/mL for C. elegans, while for C. sempervirens and clotrimazole, it was not determined within the concentration limits tested. In combination, more than 85% of the larvae survived M. alternifolia–clotrimazole, M. alternifolia–L. cubeba, C. sempervirens–clotrimazole and C. sempervirens–C. limon combinations. This study is the first, to our knowledge, to present a synergistic relationship of EO–EO and EO–clotrimazole combinations on Candida spp. biofilms.
Neonatal Morphine Results in Long-Lasting Alterations to the Gut Microbiome in Adolescence and Adulthood in a Murine Model
Authors: Antoine Danielle , Singh Praveen Kumar , Tao Junyi , Roy Sabita
Published: September 2022

Despite the many advancements in the field of pain management, the use of intravenous opioids, such as morphine, in neonates is still a challenge for clinicians and researchers, as the available evidence concerning the long-term consequences of such an early exposure is limited. In particular, little is known concerning the long-term consequences of neonatal morphine exposure on the gut microbiome, which has been identified as a key modulator of health and diseases. Consequently, the purpose of this study was to investigate those long-term consequences of neonatal morphine on the gut microbiome. Newborn mice were exposed to either morphine (5 mg/kg/day) or saline for a duration of 7 ± 2 days. Fecal samples were collected during adolescence and adulthood to longitudinally assess the gut microbiome. DNA extracted from the stool samples were sent out for 16s rRNA sequencing. During adolescence, neonatal morphine resulted in a significant increase of α-diversity and an overall decrease in the abundance of several commensal genera. During adulthood, β-diversity revealed a significantly different microbial composition of the neonatally morphine-exposed mice than that of the controls. The results demonstrate that morphine exposure during this critical developmental period resulted in long-lasting changes, particularly a reduction in several commensal bacteria. Thus, an adjunct therapeutic intervention with probiotics could potentially be used along with opioids to manage pain while attenuating the long-term co-morbidities of neonatal morphine later in life.
Transdermal Drug Delivery: Determining Permeation Parameters Using Tape Stripping and Numerical Modeling
Authors: Jonsdottir Fjola , Snorradottir Bergthora S. , Gunnarsson Skuli , Georgsdottir Elina , Sigurdsson Sven
Published: September 2022

The function of transdermal drug delivery (TDD) systems is complex due to the multiple layers necessary for controlling the rate of drug release and the interaction with the patient’s skin. In this work, we study a particular aspect of a TDD system, that is, the parameters that describe the drug permeation through the skin layers. Studies of the diffusion of two compounds were carried out and supported by tape stripping and numerical modeling. The experimental studies are carried out for porcine skin in a Franz diffusion cell and tape stripping is used to quantify the concentration of drug in the stratum corneum. A multi-layered numerical model, based on Fickian diffusion, is used to determine the unknown parameters that define the skin’s permeability, such as the partition between layers and the mass transfer coefficients due to the surface barrier. A significant correlation was found between the numerical modeling and experimental results, indicating that the partition and mass transfer effects at the interlayer boundary are accurately represented in the numerical model. We find that numerical modeling is essential to fully describe the diffusion characteristics.
Formation Thermodynamics of Carbamazepine with Benzamide, Para-Hydroxybenzamide and Isonicotinamide Cocrystals: Experimental and Theoretical Study
Authors: Manin Alex N. , Boycov Denis E. , Simonova Olga R. , Volkova Tatyana V. , Churakov Andrei V. , Perlovich German L.
Published: September 2022

Formation thermodynamic parameters for three cocrystals of carbamazepine (CBZ) with structurally related coformers (benzamide (BZA), para-hydroxybenzamide (4-OH-BZA) and isonicotinamide (INAM)) were determined by experimental (cocrystal solubility and competitive reaction methods) and computational techniques. The experimental solubility values of cocrystal components at eutectic points and solubility product of cocrystals [CBZ + BZA], [CBZ + 4-OH-BZA], and [CBZ + INAM] in acetonitrile at 293.15 K, 298.15 K, 303.15 K, 308.15 K, and 313.15 K were measured. All the thermodynamic functions (Gibbs free energy, enthalpy, and entropy) of cocrystals formation were evaluated from the experimental data. The crystal structure of [CBZ + BZA] (1:1) cocrystal was solved and analyzed by the single crystal X-ray diffractometry. A correlation between the solubility products and pure coformers solubility values has been found for CBZ cocrystals. The relationship between the entropy term and the molecular volume of the cocrystal formation has been revealed. The effectiveness of the estimation of the cocrystal formation thermodynamic parameters, based on the knowledge of the melting temperatures of active pharmaceutical ingredients, coformers, cocrystals, as well as the sublimation Gibbs energies and enthalpies of the individual components, was proven. A new method for the comparative assessment of the cocrystal stability based on the H-bond propensity analysis was proposed. The experimental and theoretical results on the thermodynamic parameters of the cocrystal formation were shown to be in good agreement. According to the thermodynamic stability, the studied cocrystals can be arranged in the following order: [CBZ + 4-OH-BZA] > [CBZ + BZA] > [CBZ + INAM].
Natural-Product-Inspired Approaches for Cancer Diagnosis and Therapy
Authors: Lee Eun Sook , Shin Jung Min
Published: September 2022

In recent years, new methods of cancer diagnosis and therapy have emerged as promising options for fighting cancer [...]
Probability Distributions for Track Fitting and the Advanced Lucky Model
Authors: Landi Gregorio , Landi Giovanni E.
Published: September 2022

Probability distributions for the center of gravity are fundamental tools for track fitting. The center of gravity is a widespread algorithm for position reconstruction in tracker detectors for particle physics. Its standard use is always accompanied by an easy guess (Gaussian) for the probability distribution of the positioning errors. This incorrect assumption degrades the results of the fit. The explicit error forms evident Cauchy–Agnesi tails that render the use of variance minimizations problematic. Therefore, it is important to report probability distributions for some combinations of random variables essential for track fitting: x=ξ/(ξ+μ), y=(ξ−μ)/[2(ξ+μ)], w=ξ/μ, x=θ(x3−x1)(−x3)/(x3+x2)+θ(x1−x3)x1/(x1+x2) and x=(x1−x3)/(x1+x2+x3). The first two are partial forms of the two strip center of gravity. The fourth is the complete two strip center of gravity, and the fifth is a partial form of the three strip center of gravity. For the complexity of the forth equation, only approximate expressions of the probability are allowed. Analytical expressions are calculated assuming ξ, μ, x1, x2 and x3 independent Gaussian random variables. The analytical form of the probability for the two strip center of gravity allows one to construct an approximate proof for the lucky model of our previous paper. This proof also suggests how to complete the lucky model by its absence of a scaling constant, relevant to combine different detector types. This advanced lucky model (the super-lucky model) can be directly used in trackers composed of non-identical detectors. The construction of the super-lucky model is very simple. Simulations with this upgraded tool also show resolution improvements for a combination of two types of very different detectors, near to the resolutions of the schematic model.
The Higher Water Absorption Capacity of Small Root System Improved the Yield and Water Use Efficiency of Maize
Authors: Yan Minfei , Zhang Li , Ren Yuanyuan , Zhang Tingting , Zhang Shaowei , Li Hongbing , Chen Yinglong , Zhang Suiqi
Published: September 2022

The root system in plants absorbs water and minerals. However, the relationship among root size, yield, and water use efficiency (WUE) is controversial. Two pot experiments were conducted to explore these relationships by using two maize varieties with contrasting root sizes and reducing the root–shoot ratio (R/S) through root pruning to eliminate genotypic effects. Maize plants were grown in an open rainout shelter under both water-sufficient and deficient conditions. Yield-related parameters, root hydraulic conductivity (Lpr), and WUE were determined. The results showed that the small root variety (XY) has a higher yield and WUE compared to large root variety (QL) under both soil moisture conditions, likely related to the higher Lpr of XY. XY also had a higher leaf water potential than QL under drought stress, indicating that small root system could provide enough water to the shoot. Further pot experiment showed that both small and large root pruning on QL (cut off about 1/5 roots, RP1; and cut off about 1/3 roots, RP2, respectively) improved WUE and Lpr, and the RP1 yield increased by 12.9% compared to the control under well-watered conditions. Root pruning decreased transpiration and increased photosynthesis. Thus, this study reveals that it is possible to increase water absorption, yield, and WUE by reducing R/S in modern maize varieties, which may be important for the future breeding of new cultivars suitable for arid regions.
Physiological and Structural Responses of Olive Leaves Related to Tolerance/Susceptibility to Verticillium dahliae
Authors: Cardoni Martina , Quero José Luis , Villar Rafael , Mercado-Blanco Jesús
Published: September 2022

Verticillium wilt of olive (VWO), caused by the soil borne fungus Verticillium dahliae, is one of the most relevant diseases affecting this crop worldwide. One of the best VWO management strategies is the use of tolerant cultivars. Scarce information is available about physiological and structural responses in the leaves of olive cultivars displaying different levels of tolerance to VWO. To identify links between this phenotype and variations in functional characteristics of the leaves, this study examined the structural and physiological traits and the correlations among them in different olive varieties. This evaluation was conducted in the presence/absence of V. dahliae. On the one hand, no leaf trait but the area was related to VWO tolerance in the absence of the pathogen. On the other hand, after inoculation, susceptible cultivars showed lower leaf area and higher leaf mass per area and dry matter content. Furthermore, at the physiological level, these plants showed severe symptoms resembling water stress. Analyzing the relationships among physiological and structural traits revealed differences between tolerant and susceptible cultivars both in the absence and in the presence of V. dahliae. These results showed that olive leaves of VWO-tolerant and VWO-susceptible cultivars adopt different strategies to cope with the pathogen.
Effects of Nitrogen and Phosphorus Regulation on Plant Type, Population Ecology and Sheath Blight of Hybrid Rice
Authors: Yang Guotao , Liu Rong , Ma Peng , Chen Hong , Zhang Rongping , Wang Xuechun , Li Yongyan , Hu Yungao
Published: September 2022

(1) Background: Sheath blight is one of the most economically significant rice diseases worldwide. A study was conducted in order to find the relationship and impact of the amount of nitrogen (N) and phosphorus (P) application on the hybrid rice population microclimate and the severity of sheath blight. (2) Methods: Four N and four P application levels were used to determine their impact on plant type, temperature, and humidity variation in different positions of population and the severity of sheath blight in the later stage. (3) Results: We found that N and P application levels could affect the plant type and change the population temperature and humidity by increasing the leaf length and leaf angle. (4) Conclusions: N application had a more significant (p < 0.05) impact on the plant type. High N application caused decreased temperature (hybrid rice population), while increased humidity (especially the population base layer at grain filling stage) resulted in severe sheath blight. High P application had similar impacts; however, P application increased material and nitrogen transport in plants and reduced the severity of sheath blight.
Effect of the Application of Hydrolysate of Chlorella vulgaris Extracted by Different Techniques on the Growth of Pelargonium × hortorum
Authors: Bayona-Morcillo Pedro José , Gómez-Serrano Cintia , González-López Cynthia Victoria , Massa Daniele , Jiménez-Becker Silvia
Published: September 2022

The extraction method used to obtain biologically active compounds from microalgal biomass may affect the biostimulant capacity of the microalgae. The objective of this assay was to determine the most efficient extraction method to release the active components of the biomass of Chlorella vulgaris (C. vulgaris). Plantlets of Pelargonium × hortorum were grown in a greenhouse and five treatments were applied: C-application with water; M-application with untreated C. vulgaris microalgae; M-US-application with C. vulgaris microalgae treated with ultrasound; M-USHY-application with C. vulgaris microalgae treated with ultrasound and enzymatic hydrolysis; and M-USHYAU-application with C. vulgaris microalgae treated with ultrasound, enzymatic hydrolysis, and autoclaving. All microalgae treatments increased shoot number and stem and plant diameter. The US-treated biomass increased the inflorescence of the plant significantly compared to the control. To extract bioactive compounds from eukaryotic microalgae for plant biostimulating purposes, the US-treatment (or any other method damaging the plasma membrane) of microalgae cell is, or seems to be, suitable.. Macronutrient content in leaves was not affected by the microalgae treatment, except for K.
Response of Bletilla striata to Drought: Effects on Biochemical and Physiological Parameter Also with Electric Measurements
Authors: Gao Yongdao , Cai Chang , Yang Qiaoan , Quan Wenxuan , Li Chaochan , Wu Yanyou
Published: September 2022

In heterogeneous landscapes with temporary water deficit characteristics in southwestern China, understanding the electrophysiological and morphological characteristics of Bletilla striata under different water conditions can help to better evaluate its suitability for planting plants in specific locations and guide planting and production. Using B. striata seedlings as experimental materials, the maximum field capacity (FC) was 75–80% (CK: control group), 50–60% FC (LS: light drought stress), 40–45% FC (MS: moderate drought stress), and 30–35% FC (SS: severe drought stress). In terms of physiological response, the activities of peroxidase (POD) and catalase (CAT) decreased under drought conditions, but the activity was well under the LS treatment, and the contents of proline (Pro) and malondialdehyde (MDA) increased. In terms of morphological responses, under drought conditions, root lengths of the rhizomes (except the LS treatment) were significantly reduced, the leaf lengths were reduced, and the biomass was significantly reduced. The stomatal size reached the maximum under the LS treatment, and the stomatal density gradually decreased with the increase in drought degree. In terms of electrophysiological responses, drought significantly decreased the net photosynthetic rate (PN) of B. striata, stomatal conductance (gs), and transpiration rate (Tr), but effectively increased the water use efficiency (WUE). The effective thickness of leaves of B. striata increased under drought conditions, and drought promoted the formation of leaf morphological diversity. Our results showed that drought stress changed the physiological and morphological characteristics of B. striata, and under light drought conditions had higher physiological activity, good morphological characteristics, higher cellular metabolic energy and ecological adaptability. Appropriate drought can promote the improvement of the quality of B. striata, and it can be widely planted in mildly arid areas.
Planoplatessa gen. nov.—A New, Neglected Monoraphid Diatom Genus with a Cavum
Authors: Kulikovskiy Maxim S. , Glushchenko Anton M. , Kuznetsova Irina V. , Kociolek John Patrick
Published: September 2022

A new monoraphid diatom genus, Planoplatessa gen. nov., is described on the basis of a detailed morphological investigation using light and scanning electron microscopy. We transferred Achnanthes joursacensis Héribaud, a species described from fossil deposits in France, to our new genus. We investigated material from Mongolia from Recent populations. This taxon is known from fossils to Recent across the Holarctic. Achnanthes joursacensis was previously transferred to the genera Planothidium and Platessa, but the morphology of A. joursacensis does not share all morphological features with these two genera. We discuss important morphological features for the delimitation of monoraphid genera based on careful morphological and molecular investigations we presented previously. Planoplatessa gen. nov. as a genus is characterized by having uniseriate striae on both the raphe and the rapheless valves, a cavum in rapheless valves only, and straight distal raphe ends on the valve face.