bluuebunny/crossref_metadata_2025 huggingface dataset:
Respiratory tract infections and risk factors for infection in a cohort of 330 patients with axial spondyloarthritis or psoriatic arthritis
Authors: Frede Natalie , Rieger Eva , Lorenzetti Raquel , Nieters Alexandra , Venhoff Ana C. , Hentze Carolin , von Deimling Marcus , Bartholomä Nora , Thiel Jens , Voll Reinhard E. , Venhoff Nils
Published: October 2022

Respiratory tract infections (RTIs) are the most common infections in patients with rheumatic diseases under immunosuppressive treatment and may contribute to morbidity and mortality as well as increased healthcare costs. However, to date only limited data on infection risk in spondyloarthritis (SpA) patients are available. In this study we assessed the occurrence of respiratory tract infections in a monocentric real-world cohort consisting of 330 patients (168 psoriatic arthritis and 162 axial spondyloarthritis patients) and determined factors associated with increased infection risk. Out of 330 SpA patients, 89.3% had suffered from ≥ 1 upper respiratory tract infection (URTI) and 31.1% from ≥ 1 lower respiratory tract infection (LRTI) within the last two years. The most common URTIs were rhinitis and laryngitis/pharyngitis with 87.3% and 36.1%, respectively. Bronchitis constituted the most common LRTI, reported in 29.7% of patients. In a multivariate binomial logistic regression model occurrence of LRTI was associated with chronic lung disease (OR 17.44, p=0.006), glucocorticoid therapy (OR 9.24, p=0.012), previous history of severe airway infections (OR 6.82, p=0.013), and number of previous biological therapies (OR 1.72, p=0.017), whereas HLA B27 positivity was negatively associated (OR 0.29, p=0.025). Female patients reported significantly more LRTIs than male patients (p=0.006) and had a higher rate of antibiotic therapy (p=0.009). There were no significant differences between axSpA and PsA patients regarding infection frequency or antibiotic use. 45.4% of patients had required antibiotics for respiratory tract infections. Antibiotic therapy was associated with smoking (OR 3.40, p=0.008), biological therapy (OR 3.38, p=0.004), sleep quality (OR 1.13, p<0.001) and age (OR 0.96, p=0.030). Hypogammaglobulinemia (IgG<7g/l) was rare (3.4%) in this SpA cohort despite continuous immunomodulatory treatment.

Awareness of these risk factors will assist physicians to identify patients with an increased infection risk, who will benefit from additional preventive measures, such as vaccination and smoking cessation or adjustment of DMARD therapy.
Identification of cerebrospinal fluid biomarker candidates for anti-N-methyl-D-aspartate receptor encephalitis: High-throughput proteomic investigation
Authors: Li Yuchen , Yang Keyu , Zhang Fang , Wang Jing , Shen Huijun , Liu Miaomiao , Guo Junhong , Wang Jie
Published: October 2022

Background

Although the diagnosis is mainly dependent on the detection of anti-N-methyl-D-aspartate receptor (NMDAR) antibodies in cerebrospinal fluid (CSF) and/or serum, there was no direct correlations between anti-NMDAR antibody titers in CSF and disease severity and prognosis in anti-NMDAR encephalitis patients. Here, we aimed to extensively identify CSF biomarkers related to the occurrence, development, and prognosis of anti-NMDAR encephalitis using a high-throughput proteomic approach.

Methods

A CSF cytokine antibody array containing 80 cytokines and inflammatory mediators related to immune and inflammatory responses was applied to identify biomarker candidates in individual CSF samples from a well-characterized cohort comprising patients with anti-NMDAR encephalitis (n = 6) and controls (n = 6). Validation and specific detection were performed in an extended cohort consisting of anti-NMDAR encephalitis patients (n = 13), controls (n = 13), and viral encephalitis (n = 13) by enzyme-linked immunosorbent assay (ELISA). Additionally, the levels of some inflammatory proteins in three groups in cohort 2 reported in previous literatures that may be involved in the development of anti-NMDAR encephalitis were also tested by ELISA. Correlations between candidate biomarkers and clinical characteristics of anti-NMDAR encephalitis patients were analyzed.

Results

Three differentially expressed cytokines and inflammatory mediators were screened from the 80-cytokine array in cohort 1. Functional enrichment analysis results suggested that these differentially expressed proteins were related to autophagy, immune/inflammatory responses, cell death, and other processes. In cohort 2, the elevations of cellular inhibitor of apoptosis protein-1 (cIAP-1), macrophage colony-stimulating factor (MCSF), CXC chemokine ligand 13 (CXCL13), and nucleotide binding oligomerization domain-like receptor protein 3 (NLRP3) in anti-NMDAR encephalitis were validated by ELISA. Linear regression revealed that the levels of CSF CXCL13 and cIAP-1 were positively correlated with the highest modified Rankin scale (mRS) score in the acute phase (p < 0.05). The level of cIAP-1 was positively correlated with the anti-NMDAR Encephalitis One-Year Functional Status (NEOS) score (p < 0.05).

Conclusion

These biomarkers show promising functions to evaluate severity or prognosis of anti-NMDAR encephalitis. The biological processes of immune/inflammatory responses, altered levels of autophagy, and the tumor necrosis factor (TNF) signal pathway may be involved in the pathophysiology of anti-NMDAR encephalitis to some extent.
NLRP3 inflammasome in digestive diseases: From mechanism to therapy
Authors: Qiang Rui , Li Yanbo , Dai Xincan , Lv Wenliang
Published: October 2022

Digestive system diseases remain a formidable challenge to human health. NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is the most characteristic multimeric protein complex and is involved in a wide range of digestive diseases as intracellular innate immune sensors. It has emerged as a research hotspot in recent years. In this context, we provide a comprehensive review of NLRP3 inflammasome priming and activation in the pathogenesis of digestive diseases, including clinical and preclinical studies. Moreover, the scientific evidence of small‐molecule chemical drugs, biologics, and phytochemicals, which acts on different steps of the NLRP3 inflammasome, is reviewed. Above all, deep interrogation of the NLRP3 inflammasome is a better insight of the pathomechanism of digestive diseases. We believe that the NLRP3 inflammasome will hold promise as a novel valuable target and research direction for treating digestive disorders.
A network view of human immune system and virus-human interaction
Authors: Tang Kang , Tang Jing , Zeng Jinfeng , Shen Wei , Zou Min , Zhang Chi , Sun Qianru , Ye Xiaoyan , Li Chunwei , Sun Caijun , Liu Siyang , Jiang Guozhi , Du Xiangjun
Published: October 2022

The immune system is highly networked and complex, which is continuously changing as encountering old and new pathogens. However, reductionism-based researches do not give a systematic understanding of the molecular mechanism of the immune response and viral pathogenesis. Here, we present HUMPPI-2022, a high-quality human protein-protein interaction (PPI) network, containing > 11,000 protein-coding genes with > 78,000 interactions. The network topology and functional characteristics analyses of the immune-related genes (IRGs) reveal that IRGs are mostly located in the center of the network and link genes of diverse biological processes, which may reflect the gene pleiotropy phenomenon. Moreover, the virus-human interactions reveal that pan-viral targets are mostly hubs, located in the center of the network and enriched in fundamental biological processes, but not for coronavirus. Finally, gene age effect was analyzed from the view of the host network for IRGs and virally-targeted genes (VTGs) during evolution, with IRGs gradually became hubs and integrated into host network through bridging functionally differentiated modules. Briefly, HUMPPI-2022 serves as a valuable resource for gaining a better understanding of the composition and evolution of human immune system, as well as the pathogenesis of viruses.
Understanding perceived effectiveness of a novel coastal management project: The case of the Bacton-Walcott sandscaping scheme, UK
Authors: Cotton Isabel , Forster Johanna , Lorenzoni Irene , Tolhurst Trevor J.
Published: October 2022

Adaptation at actively receding coastal areas requires swift and long-term solutions that build resilience for both people and the environment. Nature-based solutions are increasingly being promoted over hard defences, but there is a lack of empirical research on the effectiveness of novel approaches, including those deployed at different scales. Sandscaping, a one-off large-scale deposition of sand (1.8 M m3) on a beach frontage, was implemented for the first time in the UK at a section of beach between Bacton and Walcott villages, in North Norfolk, in 2019. The purpose of sandscaping in this location was primarily to protect the nationally important gas terminal, and neighbouring villages from coastal erosion and flooding. This study investigates the perceived effectiveness and impacts of sandscaping on coastal residents, by eliciting views of residents in the two closest villages to the scheme, and comparing findings to geomorphological observations (using LiDAR data). A survey of Bacton and Walcott residents was distributed in January 2022, with n=77 responses. Results reveal wide differences in perceptions, and notable levels of doubt, on the ‘effectiveness’ of sandscaping at present and in the future, alongside different lived experiences of the scheme and prevailing distrust by some residents about coastal management. Keeping residents updated on changes to sandscaping with environmental data and communicating the advantages of nature-based solutions appear relevant in this context, but the diversity and contrast of resident perceptions illustrates deeper challenges for future coastal management planning. There is a need to think through how future coastal change can be planned for, drawing upon multiple social perspectives. This paper also illustrates that ‘effectiveness’ of sandscaping should be more widely examined in relation to the experiences and perspectives of those impacted by the scheme, and beyond evaluations of geomorphological change.
New insights upon the reproductive biology of the sea cucumber Holothuria tubulosa (Echinodermata, Holothuroidea) in the Mediterranean: Implications for management and domestication
Authors: Pasquini Viviana , Porcu Cristina , Marongiu Martina Francesca , Follesa Maria Cristina , Giglioli Ambra Angelica , Addis Pierantonio
Published: October 2022

Holothuria tubulosa is one of the most common sea cucumber species inhabiting the Mediterranean Sea. Due to its commercial interest for the international market, it has been harvested without proper management causing the overexploitation of its stocks. Inadequate management is also caused by lack of information on basic biology and ecology not allowing the estimating of the species vulnerability and resilience to growing anthropogenic pressures. In this paper, we have investigated basic life-history traits of H. tubulosa (population structure and reproductive cycle) in a population of Central-Western Mediterranean (Sardinia, Italy). A macroscopic maturity scale for both sexes was defined through an instrumental colorimetric analysis of the gonads and the ramification level of the gonad’s tubules, subsequently confirmed by histological analysis. The seasonal trend of the Gonado-Somatic Index, the changes in color of the gonads and tubules ramification indicated that the spawning period of H. tubulosa was concentrated in summer with a peak in late August, closely related to the increase in water temperature. A synchronous development of the gonads, with a unique and short reproductive event during the year, was also detected. In conclusion, this study provides new evidence on the biological and ecological features of H. tubulosa, essential data for developing a scientifically-based stock assessment as well as conservative management at a local scale. Finally, we provided basic information for the domestication of broodstock in a conservative hatchery.
Unraveling the potential of bacteria isolated from the equatorial region of Indian Ocean in mercury detoxification
Authors: Joshi Gajendra , Verma Pankaj , Meena Balakrishnan , Goswami Prasun , Peter D Magesh , Jha Dilip Kumar , Vinithkumar Nambali Valsalan , Dharani Gopal
Published: October 2022

The marine environment is most vital and flexible with continual variations in salinity, temperature, and pressure. As a result, bacteria living in such an environment maintain the adaption mechanisms that are inherent in unstable environmental conditions. The harboring of metal-resistant genes in marine bacteria contributes to their effectiveness in metal remediation relative to their terrestrial counterparts. A total of four mercury-resistant bacteria (MRB) i.e. NIOT-EQR_J7 (Alcanivorax xenomutans); NIOT-EQR_J248 and NIOT-EQR_J251 (Halomonas sp.); and NIOT-EQR_J258 (Marinobacter hydrocarbonoclasticus) were isolated from the equatorial region of the Indian Ocean (ERIO) and identified by analyzing the 16S rDNA sequence. The MRBs can reduce up to 70% of Hg(II). The mercuric reductase (merA) gene was amplified and the mercury (Hg) volatilization was confirmed by the X-ray film method. The outcomes obtained from ICP-MS validated that the Halomonas sp. NIOT-EQR_J251 was more proficient in removing the Hg from culture media than other isolates. Fourier transform infrared (FT-IR) spectroscopy results revealed alteration in several functional groups attributing to the Hg tolerance and reduction. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis confirmed that strain Halomonas sp. (NIOT-EQR_J248 and NIOT-EQR_J251) released Isooctyl thioglycolate (IOTG) compound under mercury stress. The molecular docking results suggested that IOTG can efficiently bind with the glutathione S-transferase (GST) enzyme. A pathway has been hypothesized based on the GC-MS metabolic profile and molecular docking results, suggesting that the compound IOTG may mediate mercuric reduction via merA-GST related detoxification pathway.
Guided tissue remineralization and its effect on promoting dentin bonding
Authors: Yao Wei , Ma Liang , Chen Ruhua , Xie Yimeng , Li Bing , Zhao Bin
Published: October 2022

With the deepening of research on condensed matter chemistry, artificially guided demineralized dentin remineralization has changed from a classical remineralization pathway of the thermodynamic deposition mode to a biomimetic mineralization mode. This new mode is more consistent with the biological mineralization process. The biomimetic mineralization model can successfully simulate natural mineralization and restore the microstructure and mechanical properties of demineralized dentin. Therefore, it has a good application value in the treatment of caries and dentin hypersensitivity and adhesive restorations. This paper analyzes the principles of guided tissue remineralization and describes new research findings related to the classical mineralization model and the novel biomaterials developed using the biomimetic mineralization mode in detail. It also describes the application of these principles to improve the dentin bonding system. It thus shares the new findings in guided tissue remineralization applied to dentin bonding systems. Finally, the existing problems in this field and future development directions are proposed.
Humoral and cellular immune response after severe acute respiratory syndrome coronavirus 2 messenger ribonucleic acid vaccination in heart transplant recipients: An observational study in France
Authors: Casenaz Alice , Grosjean Sandrine , Aho-Glélé Ludwig-Serge , Bour Jean-Baptiste , Auvray Christelle , Manoha Catherine
Published: October 2022

Introduction

Heart transplant (HT) recipients have a high risk of developing severe COVID-19. Immunoglobulin G antibodies are considered to provide protective immunity and T-cell activity is thought to confer protection from severe disease. However, data on T-cell response to mRNA vaccination in a context of HT remains limited.

Methods

In 96 HT patients, a IFN-γ release assay and an anti-Spike antibody test were used to evaluate the ability of SARS-CoV-2 mRNA vaccines to generate cellular and humoral immune response. Blood samples were collected few weeks to 7 months after vaccination. Multiple fractional polynomial and LASSO regression models were used to define predictors of T-cell response.

Results

Three to five months after vaccination, three doses of vaccine induced a positive SARS-CoV-2 T-cell response in 47% of recipients and a positive humoral response in 83% of recipients, 11.1% of patients remained negative for both T and B cell responses. Three doses were necessary to reach high IgG response levels (>590 BAU/mL), which were obtained in a third of patients. Immunity was greatly amplified in the group who had three vaccine doses plus COVID-19 infection.

Conclusion

Our study revealed that T and B immunity decreases over time, leading us to suggest the interest of a booster vaccination at 5 months after the third dose. Moreover, a close follow-up of immune response following vaccination is needed to ensure ongoing immune protection. We also found that significant predictors of higher cellular response were infection and active smoking, regardless of immunosuppressive treatment with mycophenolate mofetil (MMF).
A literature review on the potential clinical implications of streptococci in gastric cancer
Authors: Zi Mengli , Zhang Yanqiang , Hu Can , Zhang Shengjie , Chen Jinxia , Yuan Li , Cheng Xiangdong
Published: October 2022

Streptococcus is widely found in nature and the human body, and most species are not pathogenic. In recent years, studies have found that Streptococcus is associated with gastric cancer. Streptococcus was found to be enriched in the oral cavity, stomach and intestine of gastric cancer patients and found to be increased in gastric cancer tissues, suggesting that Streptococcus may be the pathogenic bacteria underlying gastric cancer. This review discusses the discovery of Streptococcus, the relationship between Streptococcus and gastric cancer, and the possible carcinogenic mechanism of Streptococcus and summarizes the progress of the research on the role of Streptococcus in gastric cancer to provide new ideas for the early detection, diagnosis and treatment of gastric cancer.
Viruses in astrobiology
Authors: de la Higuera Ignacio , Lázaro Ester
Published: October 2022

Viruses are the most abundant biological entities on Earth, and yet, they have not received enough consideration in astrobiology. Viruses are also extraordinarily diverse, which is evident in the types of relationships they establish with their host, their strategies to store and replicate their genetic information and the enormous diversity of genes they contain. A viral population, especially if it corresponds to a virus with an RNA genome, can contain an array of sequence variants that greatly exceeds what is present in most cell populations. The fact that viruses always need cellular resources to multiply means that they establish very close interactions with cells. Although in the short term these relationships may appear to be negative for life, it is evident that they can be beneficial in the long term. Viruses are one of the most powerful selective pressures that exist, accelerating the evolution of defense mechanisms in the cellular world. They can also exchange genetic material with the host during the infection process, providing organisms with capacities that favor the colonization of new ecological niches or confer an advantage over competitors, just to cite a few examples. In addition, viruses have a relevant participation in the biogeochemical cycles of our planet, contributing to the recycling of the matter necessary for the maintenance of life. Therefore, although viruses have traditionally been excluded from the tree of life, the structure of this tree is largely the result of the interactions that have been established throughout the intertwined history of the cellular and the viral worlds. We do not know how other possible biospheres outside our planet could be, but it is clear that viruses play an essential role in the terrestrial one. Therefore, they must be taken into account both to improve our understanding of life that we know, and to understand other possible lives that might exist in the cosmos.
Rhizospheric microbial consortium of Lilium lancifolium Thunb. causes lily root rot under continuous cropping system
Authors: Dai Liangliang , Singh Sunil K. , Gong Hao , Tang Yuanyuan , Peng Zhigang , Zhang Jun , Wu Dousheng , Zhang Huiming , He Danxia
Published: October 2022

Tiger lily (Lilium lancifolium Thunb.) is a cash crop with a long history of cultivation in China. Its roots have long been used as a valuable component of Chinese medicine. Continuous cropping, the conventional planting approach for tiger lily, often leads to severe root rot disease, but it is not yet clear how this planting method leads to root rot. In this study, we analyzed the rhizosphere microbiome and predicted microbial protein function in tiger lily planted with the continuous cropping method in three different geological types of soil. In order to explore the specific rhizosphere microbiota triggering root rot disease, tiger lily was compared to maize grown in a similar system, which showed no disease development. An analysis of the chemical elements in the soil revealed that the Pseudomonas and Streptomyces genera, with pathogenic functions, were dominant in the tiger lily rhizosphere. The lower soil pH of tiger lily compared to maize supports the accumulation of pathogenic bacteria in the tiger lily rhizosphere. Meanwhile, we discovered that bacteria of the Flavobacterium genus, with their predicted phosphate transport function, specifically accumulated in the maize rhizosphere. Our findings suggest that Pseudomonas and Streptomyces bacteria may result in continuous cropping–induced root rot disease in tiger lily and that Flavobacterium could serve to protect maize from pathogenic bacteria.
Stable binding to phosphatidylserine-containing membranes requires conserved arginine residues in tandem C domains of blood coagulation factor VIII
Authors: Peters Shaun C. , Childers Kenneth C. , Mitchell Corbin E. , Avery Nathan G. , Reese Steven S. , Mitchell Cristopher , Wo Serena W. , Swanson Christopher D. , Brison Caileen M. , Spiegel P. Clint
Published: October 2022

At sites of vascular damage, factor VIII (fVIII) is proteolytically activated by thrombin and binds to activated platelet surfaces with activated factor IX (fIXa) to form the intrinsic “tenase” complex. Previous structural and mutational studies of fVIII have identified the C1 and C2 domains in binding to negatively charged membrane surfaces through β-hairpin loops with solvent-exposed hydrophobic residues and a ring of positively charged basic residues. Several hemophilia A-associated mutations within the C domains are suggested to disrupt lipid binding, preventing formation of the intrinsic tenase complex. In this study, we devised a novel platform for generating recombinant C1, C2, and C1C2 domain constructs and performed mutagenesis of several charged residues proximal to the putative membrane binding region of each C domain. Binding measurements between phosphatidylserine (PS)-containing lipid membrane surfaces and fVIII C domains demonstrated an ionic strength dependence on membrane binding affinity. Mutations to basic residues adjacent to the surface-exposed hydrophobic regions of C1 and C2 differentially disrupted membrane binding, with abrogation of binding occurring for mutations to conserved arginine residues in the C1 (R2163) and C2 (R2320) domains. Lastly, we determined the X-ray crystal structure of the porcine fVIII C2 domain bound to o-phospho-L-serine, the polar headgroup of PS, which binds to a basic cleft and makes charge-charge contact with R2320. We conclude that basic clefts in the fVIII C domains bind to PS-containing membranes through conserved arginine residues via a C domain modularity, where each C domain possesses modest electrostatic-dependent affinity and tandem C domains are required for high affinity binding.
Tissue management in precision medicine: What the pathologist needs to know in the molecular era
Authors: Souza da Silva Ricella , Pinto Regina , Cirnes Luis , Schmitt Fernando
Published: October 2022

Precision medicine is “an emerging approach for disease treatment and prevention that takes into account individual variability in genes, environment, and lifestyle for each person.” Among many medical specialists involved in precision medicine, the pathologists play an important and key role in the implementation and development of molecular tests that are in the center of decision of many therapeutic choices. Besides many laboratory procedures directly involved in the molecular tests, is fundamental to guarantee that tissues and cells collected for analysis be managed correctly before the DNA/RNA extraction. In this paper we explore the pivotal and interconnected points that can influence molecular studies, such as pre-analytical issues (fixation and decalcification); diagnosis and material selection, including the calculation of nuclei neoplastic fraction. The standardization of sample processing and morphological control ensures the accuracy of the diagnosis. Tissue or cytological samples constitutes the main foundation for the determination of biomarkers and development of druggable targets. Pathology and precision oncology still have a long way to go in terms of research and clinical practice: improving the accuracy and dissemination of molecular tests, learning in molecular tumor boards for advanced disease, and knowledge about early disease. Precision medicine needs pathology to be precise.
Topological patterns of motor networks in Parkinson’s disease with different sides of onset: A resting-state-informed structural connectome study
Authors: Zhang Xiuli , Li Ruohan , Xia Yingying , Zhao Houliang , Cai Lulu , Sha Jingyun , Xiao Qihua , Xiang Jie , Zhang Chao , Xu Kai
Published: October 2022

Parkinson’s disease (PD) has a characteristically unilateral pattern of symptoms at onset and in the early stages; this lateralization is considered a diagnostically important diagnosis feature. We aimed to compare the graph-theoretical properties of whole-brain networks generated by using resting-state functional MRI (rs-fMRI), diffusion tensor imaging (DTI), and the resting-state-informed structural connectome (rsSC) in patients with left-onset PD (LPD), right-onset PD (RPD), and healthy controls (HCs). We recruited 26 patients with PD (13 with LPD and 13 with RPD) as well as 13 age- and sex-matched HCs. Rs-fMRI and DTI were performed in all subjects. Graph-theoretical analysis was used to calculate the local and global efficiency of a whole-brain network generated by rs-fMRI, DTI, and rsSC. Two-sample t-tests and Pearson correlation analysis were conducted. Significantly decreased global and local efficiency were revealed specifically in LPD patients compared with HCs when the rsSC network was used; no significant intergroup difference was found by using rs-fMRI or DTI alone. For rsSC network analysis, multiple network metrics were found to be abnormal in LPD. The degree centrality of the left precuneus was significantly correlated with the Unified Parkinson’s Disease Rating Scale (UPDRS) score and disease duration (p = 0.030, r = 0.599; p = 0.037, r = 0.582). The topological properties of motor-related brain networks can differentiate LPD and RPD. Nodal metrics may serve as important structural features for PD diagnosis and monitoring of disease progression. Collectively, these findings may provide neurobiological insights into the lateralization of PD onset.
Synergistic influence of cytokine gene polymorphisms over the risk of dementia: A multifactor dimensionality reduction analysis
Authors: Juárez-Cedillo Teresa , Martínez-Rodríguez Nancy , Vargas-Alarcon Gilberto , Juárez-Cedillo Enrique , Valle-Medina Antonio , Garrido-Acosta Osvaldo , Ramirez Alfredo
Published: October 2022

Objective

Evidence supports the important role of neuroinflammation in some types of dementia. This study aimed to evaluate the effect of epistasis of gene cytokines such as interleukin (IL)-α, IL-6, tumor necrosis factor (TNFα), and interferon-gamma (IFN-γ) on the susceptibility to the development of dementia.

Materials and methods

In the study, 221 patients diagnosed with dementia and 710 controls were included. The multifactor-dimensionality reduction (MDR) analysis was performed to identify the epistasis between SNP located in genes of IL-α (rs1800587), IL-6 (rs1800796), TNFα (rs361525 and rs1800629), and IFNγ (rs2069705). The best risk prediction model was identified based on precision and cross-validation consistency.

Results

Multifactor-dimensionality reduction analysis detected a significant model with the genes TNFα, IFNγ, IL1α, and IL6 (prediction success: 72%,p< 0.0001). When risk factors were analyzed with these polymorphisms, the model achieved a similar prediction for dementia as the genes-only model.

Conclusion

These data indicate that gene–gene interactions form significant models to identify populations susceptible to dementia.
Assessment methodology for human-exoskeleton interactions: Kinetic analysis based on muscle activation
Authors: Fanti Vasco , Sanguineti Vittorio , Caldwell Darwin G. , Ortiz Jesús , Di Natali Christian
Published: October 2022

During the development and assessment of an exoskeleton, many different analyzes need to be performed. The most frequently used evaluate the changes in muscle activations, metabolic consumption, kinematics, and kinetics. Since human-exoskeleton interactions are based on the exchange of forces and torques, the latter of these, kinetic analyzes, are essential and provide indispensable evaluation indices. Kinetic analyzes, however, require access to, and use of, complex experimental apparatus, involving many instruments and implicating lengthy data analysis processes. The proposed methodology in this paper, which is based on data collected via EMG and motion capture systems, considerably reduces this burden by calculating kinetic parameters, such as torque and power, without needing ground reaction force measurements. This considerably reduces the number of instruments used, allows the calculation of kinetic parameters even when the use of force sensors is problematic, does not need any dedicated software, and will be shown to have high statistical validity. The method, in fact, combines data found in the literature with those collected in the laboratory, allowing the analysis to be carried out over a much greater number of cycles than would normally be collected with force plates, thus enabling easy access to statistical analysis. This new approach evaluates the kinetic effects of the exoskeleton with respect to changes induced in the user's kinematics and muscular activation patterns and provides indices that quantify the assistance in terms of torque (AMI) and power (API). Following the User-Center Design approach, which requires driving the development process as feedback from the assessment process, this aspect is critical. Therefore, by enabling easy access to the assessment process, the development of exoskeletons could be positively affected.
Comparative efficacy of acupuncture-related therapy for migraine: A systematic review and network meta-analysis
Authors: Song Yanjuan , Li Tong , Ma Chunlian , Liu Hui , Liang Fengxia , Yang Yi
Published: October 2022

Background

Migraine is a worldwide disabling chronic brain disorder, some studies suggest acupuncture-related therapy plays an important role in raising efficiency rates and reducing migraine attacks. However, clinical trials comparing the efficacy of different interventions for migraine are limited and controversial. This network meta-analysis (NMA) was performed to review all randomized controlled trials (RCTs) comparing the effects of acupuncture-related therapy for migraine.

Methods

Randomized controlled trials (RCTs) of acupuncture-related therapy for migraine were searched in the following databases from the date of database inception to March 31, 2022, including PubMed, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure (CNKI), VIP Database, Wanfang Database, and Chinese Biomedical Database (CBM). The primary endpoint was visual analog scale (VAS) scores. The secondary endpoints were the number of migraine days, duration of migraine, and frequency of migraine attacks. We used Cochrane risk of bias to assess the quality of evidence for outcomes.

Results

Thirty-nine studies involving 4379 patients with 13 different acupuncture-related methods were evaluated. According to surface under the cumulative ranking curve value, acupoint injection was ranked the highest (98.0%) in VAS scores, followed by acupoint implantation (79.0%); electroacupuncture was the optimal intervention method (82.4%) in the number of migraine days, followed by embedding needle therapy (73.1%); embedding needle therapy ranked first (99.9%) in the duration of migraine, followed by acupoint injection (77.4%); acupoint injection was the best intervention (99.3%) in the frequency of migraine attacks, followed by conventional acupuncture plus massage (73.8%).

Conclusion

These results provide preliminary evidence that acupuncture-related therapy could be recommended as one of the effective treatments for migraine. Conventional acupuncture has significant effects on improving VAS scores, the number of migraine days, duration of migraine, and frequency of migraine attacks. However, more high-quality studies should be carried out to verify this finding.

Systematic review registration

https://inplasy.com/, identifier: INPLASY202110035.
Bloody fluids located between the temporal muscle and targeted cerebral cortex affect the establishment of indirect collaterals in Moyamoya disease with surgical bypass: A case-control study
Authors: Li Yin , Hu Jun-wen , He Xu-chao , Cao Yang , Yu Xiao-bo , Fu Xiong-jie , Zhou Hang , Hu Li-bin , Xu Liang , Xu Chao-ran , Wang Yong-jie , Wang Lin
Published: October 2022

Objective

Bypass yields favorable outcomes in the treatment of Moyamoya disease (MMD). Bloody fluids accumulate between the targeted cortex and the temporal muscle after surgical bypass. These fluids are handled empirically via subcutaneous tubes or conservative treatments. However, substances located in certain positions may adversely affect the establishment of indirect collaterals (ICs) from muscular grafts.

Methods

Patients in our hospital from January 2014 to December 2019 were eligible for inclusion. Digital subtraction angiography (DSA) and radiological examinations were used during the perioperative and follow-up periods. Bloody fluid volumes were calculated using computed tomography- (CT-) based 3D Slicer software. The characteristics of bloody fluids, patient demographics, and clinical data were retrospectively analyzed.

Results

In total, 110 patients underwent indirect or combined bypass with follow-up DSA. The mean age of the enrolled patients was 42.4 ± 11.8 years. Previous ischemia (p = 0.001), previous hemorrhage (p = 0.013), bloody fluid volume (p = 0.049), and the time of imaging (p = 0.081) were associated with indirect outcomes. Ordinal regression analysis confirmed that good indirect outcomes were associated with previous ischemia (p < 0.001) and a large bloody fluid volume (p = 0.013). Further subgroups based on fluid volume were significantly correlated with IC establishment (p = 0.030).

Conclusions

A large bloody fluid volume and previous ischemic history were associated with good indirect outcomes. The presence of bloody fluids may reflect impaired degrees of muscular donors due to bipolar electrocoagulation, thus highlighting the importance of appropriate application of bipolar forceps.
Evaluation of hidden hearing loss in normal-hearing firearm users
Authors: Grinn Sarah K. , Le Prell Colleen G.
Published: October 2022

Some noise exposures resulting in temporary threshold shift (TTS) result in cochlear synaptopathy. The purpose of this retrospective study was to evaluate a human population that might be at risk for noise-induced cochlear synaptopathy (i.e., “hidden hearing loss”). Participants were firearm users who were (1) at-risk for prior audiometric noise-induced threshold shifts, given their history of firearm use, (2) likely to have experienced complete threshold recovery if any prior TTS had occurred, based on this study’s normal-hearing inclusion criteria, and (3) not at-risk for significant age-related synaptopathic loss, based on this study’s young-adult inclusion criteria. 70 participants (age 18–25 yr) were enrolled, including 33 firearm users experimental (EXP), and 37 non-firearm users control (CNTRL). All participants were required to exhibit audiometric thresholds ≤20 dB HL bilaterally, from 0.25 to 8 kHz. The study was designed to test the hypothesis that EXP participants would exhibit a reduced cochlear nerve response compared to CNTRL participants, despite normal-hearing sensitivity in both groups. No statistically significant group differences in auditory performance were detected between the CNTRL and EXP participants on standard audiom to etry, extended high-frequency audiometry, Words-in-Noise performance, distortion product otoacoustic emission, middle ear muscle reflex, or auditory brainstem response. Importantly, 91% of EXP participants reported that they wore hearing protection either “all the time” or “almost all the time” while using firearms. The data suggest that consistent use of hearing protection during firearm use can effectively protect cochlear and neural measures of auditory function, including suprathreshold responses. The current results do not exclude the possibility that neural pathology may be evident in firearm users with less consistent hearing protection use. However, firearm users with less consistent hearing protection use are also more likely to exhibit threshold elevation, among other cochlear deficits, thereby confounding the isolation of any potentially selective neural deficits. Taken together, it seems most likely that firearm users who consistently and correctly use hearing protection will exhibit preserved measures of cochlear and neural function, while firearm users who inconsistently and incorrectly use hearing protection are most likely to exhibit cochlear injury, rather than evidence of selective neural injury in the absence of cochlear injury.
Reconstruction of perceived face images from brain activities based on multi-attribute constraints
Authors: Hou Xiaoyuan , Zhao Jing , Zhang Hui
Published: October 2022

Reconstruction of perceived faces from brain signals is a hot topic in brain decoding and an important application in the field of brain-computer interfaces. Existing methods do not fully consider the multiple facial attributes represented in face images, and their different activity patterns at multiple brain regions are often ignored, which causes the reconstruction performance very poor. In the current study, we propose an algorithmic framework that efficiently combines multiple face-selective brain regions for precise multi-attribute perceived face reconstruction. Our framework consists of three modules: a multi-task deep learning network (MTDLN), which is developed to simultaneously extract the multi-dimensional face features attributed to facial expression, identity and gender from one single face image, a set of linear regressions (LR), which is built to map the relationship between the multi-dimensional face features and the brain signals from multiple brain regions, and a multi-conditional generative adversarial network (mcGAN), which is used to generate the perceived face images constrained by the predicted multi-dimensional face features. We conduct extensive fMRI experiments to evaluate the reconstruction performance of our framework both subjectively and objectively. The results show that, compared with the traditional methods, our proposed framework better characterizes the multi-attribute face features in a face image, better predicts the face features from brain signals, and achieves better reconstruction performance of both seen and unseen face images in both visual effects and quantitative assessment. Moreover, besides the state-of-the-art intra-subject reconstruction performance, our proposed framework can also realize inter-subject face reconstruction to a certain extent.
Electroacupuncture relieves hyperalgesia by regulating neuronal–glial interaction and glutamate transporters of spinal dorsal horns in rats with acute incisional neck pain
Authors: Wang Jun-ying , Zhang Jin-ling , Chen Shu-ping , Gao Yong-hui , Zhang Jian-liang , Chen Yu , Zhang Yue , Rong Pei-jing , Liu Jun-ling
Published: October 2022

Objective

Glial cells are involved in the analgesic effect of electroacupuncture (EA) in rats with chronic neurological pain. The objective of this study was to observe the role of neuronal–glial interaction and glutamate (Glu) transporters in EA-induced acute neck pain relief in rats.

Materials and methods

Male rats were placed into the following five groups: control, model, EA Futu (LI18), EA Hegu (LI4)-Neiguan (PC6), and EA Zusanli (ST36)-Yanglingquan (GB34). The incisional neck pain model was established by making a longitudinal incision along the midline of the neck. The thermal pain threshold (TPT) was measured using a radiation heat detector. The immunoactivities of glial fibrillary acidic protein (GFAP), ionized calcium-binding adapter molecule 1 (Iba-1), neurokinin-1 receptor (NK-1R), Glu aspartate transporter (GLAST), and Glu transporter-1 (GLT-1) in the dorsal horns (DHs) of the cervico-spinal cord (C2–C5) were detected using immunofluorescence histochemistry. The expression levels of GFAP, Iba-1, GLAST, and GLT-1 mRNAs were determined using quantitative real-time polymerase chain reaction (PCR).

Results

The TPT and levels of mRNAs expression and immunoactivity of GLT-1 and GLAST were significantly decreased, and those of Iba-1 and GFAP were significantly increased in the model group than those of the control group (P < 0.05). The activated microgliacytes were gathered around the NK-1R positive neurons, and co-expression of NK-1R and astrocytes was observed in the model group. EA LI18 significantly increased the TPT and expression of GLAST and GLT-1 mRNAs (P < 0.05) and notably decreased the number of Iba-1 positive cells and Iba-l mRNA expression (P < 0.05), whereas GLAST and GLT-1 antagonists inhibited the analgesic effect of EA LI18. However, these effects, except for the downregulation of Iba-1 mRNA, were not observed in the EA ST36-GB34 group. Fewer NK-1R-positive neurons were visible in the spinal DHs in the EA LI18 group, and the co-expression of NK-1R and astrocytes was also lower than that in the three EA groups.

Conclusion

Electroacupuncture of LI18 had an analgesic effect in rats with neck incisions, which may be related to its functions in suppressing the neuronal–glial cell interaction through NK-1R and upregulating the expression of GLAST and GLT-1 in the spinal DHs.
New multiple sclerosis lesion segmentation and detection using pre-activation U-Net
Authors: Ashtari Pooya , Barile Berardino , Van Huffel Sabine , Sappey-Marinier Dominique
Published: October 2022

Automated segmentation of new multiple sclerosis (MS) lesions in 3D MRI data is an essential prerequisite for monitoring and quantifying MS progression. Manual delineation of such lesions is time-consuming and expensive, especially because raters need to deal with 3D images and several modalities. In this paper, we propose Pre-U-Net, a 3D encoder-decoder architecture with pre-activation residual blocks, for the segmentation and detection of new MS lesions. Due to the limited training set and the class imbalance problem, we apply intensive data augmentation and use deep supervision to train our models effectively. Following the same U-shaped architecture but different blocks, Pre-U-Net outperforms U-Net and Res-U-Net on the MSSEG-2 dataset, achieving a Dice score of 40.3% on new lesion segmentation and an F1 score of 48.1% on new lesion detection. The codes and trained models are publicly available at https://github.com/pashtari/xunet.
Structural characteristics of polysaccharide from Zingiber striolatum and its effects on gut microbiota composition in obese mice
Authors: Jiang Wei , Hu Ying , Zhu Zhenyuan
Published: October 2022

To investigate a polysaccharide from Zingiber striolatum favorably modulates gut microbiota in mice fed a high-fat diet. Z. striolatum was utilized to extract the crude polysaccharide CZSP, which was subsequently refined using DEAE-52 cellulose and Sephadex G-150 to yield the novel polysaccharide Zingiber strioatum pure polysaccharide-1 (ZSPP-1). ZSPP-1 was an acidic heteroglycan made up of galactose, mannose, glucose, xylose, arabinose, glucuronic acid, and galacturonic acid with an average molecular weight of 1.57 × 106 Da. The structure of ZSPP-1 was investigated by FT-IR, methylation and NMR analysis, and the results denoted that the linkage structure types include T-Manp-linked, β-Xylp-(1,2)-linked, β-Galp-(1,4)-linked, α-GlcpA-(1,6)-linked, β-Arap-(1,4)-linked, α-Glcp-(1,3,4,6)-linked, α-Glcp-(1,2)-linked, and β-T-Xylp-linked, in which β-Galp-(1,4)-linked and α-GalpA-(1,4)-linked might be the main linkage. The results of the intervention experiments showed that ZSPP-1 changed the intestinal flora structure of the Firmicutes and Bacteroidetes in obese mice, and promoted the growth of beneficial bacteria such as Akkermansia, Lactobacillus, and Bacteroides in the intestine. It also restored the imbalanced flora structure due to high-fat diet to normal. It also restored the imbalanced flora structure due to high-fat diet to normal. Z. striolatum polysaccharides presented a considerable advantage in alleviating high-fat diet induced obesity, which indicates that it can be further exploited as a natural functional food resource.