bluuebunny/crossref_metadata_2025 huggingface dataset:
Pelvic abscess formation in perforated carcinoma of the jejunum
Authors: Peh Wilfred C.G. , Ho Judy W.C.
Published: August 2019

A 76 year old Chinese woman presented with a pelvic abscess, secondary to perforated carcinoma of the jejunum. Plain abdominal radiograph showed a mottled lesion which corresponded to the site of the perforated tumour seen on CT and confirmed during laparotomy. The clinical and imaging features of carcinoma of the jejunum are briefly reviewed.
Pseudotumours of the liver: Diaphragmatic slip simulating hepatic metastases
Authors: Van Gelderen W. F.C. , Williams T. M.
Published: August 2019

The radiological features of a diaphragmatic slip in an unusual location and simulating liver metastases on computed tomography (CT) are described and the relevant literature is reviewed. The fact that the features so closely mimicked metastases on CT and that ultrasound so readily excluded this possibility, prompted this report.
Digital radiology at the 1996 RSNA Congress
Authors: Beningfield S. J.
Published: August 2019

No abstract available.
Obituary: Derek Harwood-Nash
Authors: Van Niekerk J.P. De V. , Cremin B. J.
Published: August 2019

No abstract available.
Editorial
Authors: Beningfield S. J.
Published: August 2019

No abstract available.
Unusual presentation of jejunal hemangioma on Tc-99m pertechnetate scan with single-photon emission computerized tomography-computed tomography
Authors: Peepre Karan , Borkar Nitinkumar , Jondhale Sunil N. , Ravina Mudalsha , Moideen Amal , Yadav Vipin , Dey Sushmita
Published: August 2019

Small bowel hemangioma is a rare benign tumor in the pediatric population. The usual presentation of these tumors is melena, anemia, or hematochezia. Our case demonstrates the usefulness of Meckel's/Tc-99m pertechnetate scan with single-photon emission computerized tomography/computerized tomography in diagnosing a vascular lesion in the small bowel in a child presenting with melena, unresponsive to medical management. We present a case of incidentally detected jejunal hemangioma during Tc-99m pertechnetate scintigraphy which would help the nuclear medicine physician and surgeon, to be cognizant of this atypical presentation in their clinical practice.
Statistical analysis of the occupational radiation doses in three different positron emission tomography–computed tomography centers in Egypt
Authors: Saad Ahmed Ibrahim Elsayed , Zamzam Abdulrahman M.M. , Yassin Hossam Mahmoud
Published: August 2019

In the present study, we investigated the radiation doses received by the positron emission tomography (PET)/computed tomography (CT) staff in three different diagnostic centers in Egypt. The whole-body effective dose measured by thermoluminescent dosimeters (TLDs) for staff working in PET and the effective dose per study received by physicist, technician, and nurse were measured by an electronic pocket dosimeter (EPD) during a period of 6 months. Statistical analysis was held between the measurements of the TLDs as well as for the EPD for the three studied PET-CT centers. After combining TLD and EPD prospective annual scores for the three studied categories in the three centers, the one-way ANOVA test results have shown that there were statistically significant differences between group means with respect to their TLD mean score (P = 0.041). The mean nurse group TLD score, across the three centers, appeared to be the lowest scoring 3.83 (standard deviation [SD] 0.012) compared to the physicist and technician who measured 4.62 (SD 0.231) and 6.92 (SD 0.018), respectively. Scheffe's test for complex comparisons revealed a significant difference between nurse group and technologist group (P = 0.001). Regarding the annual combined EPD scores, the post hoc test, namely Scheffe's test for complex comparisons, revealed a significant difference between nurse group and technologist group (P = 0.001). This was measured after the one-way ANOVA test results have shown that there were statistically significant differences between annual group EPD means (P = 0.032). Finally, there was no recorded significance for the studied categories across the three centers between their annual TLD and EPD dose scores (P = 0.072). Technicians group received the highest mean effective whole-body doses when compared with the International Commission on Radiological Protection dose limit, each individual worker can work with many more 18F-fluorodeoxyglucose (FDG) PET/CT studies for a (period time) without exceeding the occupational dose limits if the average received effective dose continues with the same rate. The study also confirmed that low levels of radiation dose are received by medical personnel involved in 18F-FDG PET/CT procedures in those centers due to implementing radiation protection measures and procedures.
Predictors for Mortality in Children with Scorpion Envenomation Admitted to Pediatric Intensive Care Unit, Qena Governorate, Egypt
Authors: Baseer Khaled A. , Naser Mohamed A. A.
Published: August 2019

This study aimed to identify the clinical and laboratory manifestations that affect outcome of scorpion envenomation in children. It included 154 children admitted with scorpion sting envenomation over a period of 2 years. The epidemiological, clinical, and laboratory findings of patients were recorded, and grading of severity was performed based on local and systemic involvement. Organ failure was determined according to diagnostic criterion of multiple organ dysfunction syndrome, and severity of illness was assessed by the Pediatric Risk of Mortality (PRISM III) score. Of studied children, 58.4% were males and 41.6% were females. Children aged > 5 years suffered more scorpion stings (79.9%) than others did. The place of residence was rural more than urban, outdoor stings more than indoors, nocturnal more than diurnal, and most stings were on the exposed areas of the limbs. Based on clinical evaluation, 37.7% of patients were classified as class I severity followed by class II (48.7%) and class III (13.6%). Among studied cases, 21 deaths (13.6%) were registered; all of them belonged to class III severity. Mortality was significantly higher in children with agitation, coma, convulsions, arrhythmia, heart failure, pulmonary edema, and priapism. There were significantly higher values of leukocytes, platelets, creatinine, liver enzymes, glucose, and creatine phosphokinase in non-survivors than in survivors. The presence of organ failure was associated with mortality. In addition, the need for mechanical ventilation and inotropic support were at increased risk of mortality. Moreover, a significant association was found between PRISM score and the number of failed organs with fatal outcome.
Biogenic and anthropogenic sources of aerosols at the High Arctic site Villum Research Station
Authors: Nielsen Ingeborg E. , Skov Henrik , Massling Andreas , Eriksson Axel C. , Dall'Osto Manuel , Junninen Heikki , Sarnela Nina , Lange Robert , Collier Sonya , Zhang Qi , Cappa Christopher D. , Nøjgaard Jacob K.
Published: August 2019

Abstract. There are limited measurements of the chemical
composition, abundance and sources of atmospheric particles in the High
Arctic To address this, we report 93 d of soot particle aerosol mass
spectrometer (SP-AMS) data collected from 20 February to 23 May
2015 at Villum Research Station (VRS) in northern Greenland
(81∘36′ N). During this period, we observed the Arctic haze
phenomenon with elevated PM1 concentrations ranging from an average of
2.3, 2.3 and 3.3 µg m−3 in February, March and April, respectively, to 1.2 µg m−3 in May. Particulate sulfate (SO42-) accounted
for 66 % of the non-refractory PM1 with the highest concentration until
the end of April and decreasing in May. The second most abundant species was
organic aerosol (OA) (24 %). Both OA and PM1, estimated from the sum
of all collected species, showed a marked decrease throughout May in
accordance with the polar front moving north, together with changes in
aerosol removal processes. The highest refractory black carbon (rBC)
concentrations were found in the first month of the campaign, averaging 0.2 µg m−3. In March and April, rBC averaged 0.1 µg m−3 while decreasing to 0.02 µg m−3 in May. Positive matrix factorization (PMF) of the OA mass spectra yielded three
factors: (1) a hydrocarbon-like organic aerosol (HOA) factor, which was
dominated by primary aerosols and accounted for 12 % of OA mass, (2) an
Arctic haze organic aerosol (AOA) factor and (3) a more oxygenated marine
organic aerosol (MOA) factor. AOA dominated until mid-April (64 %–81 %
of OA), while being nearly absent from the end of May and correlated
significantly with SO42-, suggesting the main part of that factor
is secondary OA. The MOA emerged late at the end of March, where it
increased with solar radiation and reduced sea ice extent and dominated OA
for the rest of the campaign until the end of May (24 %–74 % of OA), while
AOA was nearly absent. The highest O∕C ratio (0.95) and S∕C ratio (0.011)
was found for MOA. Our data support the current understanding that Arctic
aerosols are highly influenced by secondary aerosol formation and receives an
important contribution from marine emissions during Arctic spring in remote
High Arctic areas. In view of a changing Arctic climate with changing
sea-ice extent, biogenic processes and corresponding source strengths,
highly time-resolved data are needed in order to elucidate the components
dominating aerosol concentrations and enhance the understanding of the
processes taking place.
Causal networks of biosphere–atmosphere interactions
Authors: Krich Christopher , Runge Jakob , Miralles Diego G. , Migliavacca Mirco , Perez-Priego Oscar , El-Madany Tarek , Carrara Arnaud , Mahecha Miguel D.
Published: August 2019

Abstract. Local meteorological conditions and biospheric activity are tightly coupled. Understanding these links is an essential prerequisite for predicting the Earth system under climate change conditions. However, many empirical studies on the interaction between the biosphere and the atmosphere are based on correlative approaches that are not able to deduce causal paths, and only very few studies apply causal discovery methods. Here, we use a recently proposed causal graph discovery algorithm, which aims to reconstruct the causal dependency structure underlying a set of time series. We explore the potential of this method to infer temporal dependencies in biosphere-atmosphere interactions. Specifically we address the following questions: How do periodicity and heteroscedasticity influence causal detection rates, i.e. the detection of existing and non-existing links? How consistent are results for noise-contaminated data? Do results exhibit an increased information content that justifies the use of this causal-inference method? We explore the first question using artificial time series with well known dependencies that mimic real-world biosphere-atmosphere interactions. The two remaining questions are addressed jointly in two case studies utilizing observational data. Firstly, we analyse three replicated eddy covariance datasets from a Mediterranean ecosystem at half hourly time resolution allowing us to understand the impact of measurement uncertainties. Secondly, we analyse global NDVI time series (GIMMS 3g) along with gridded climate data to study large-scale climatic drivers of vegetation greenness. Overall, the results confirm the capacity of the causal discovery method to extract time-lagged linear dependencies under realistic settings. The violation of the method's assumptions increases the likelihood to detect false links. Nevertheless, we consistently identify interaction patterns in observational data. Our findings suggest that estimating a directed biosphere-atmosphere network at the ecosystem level can offer novel possibilities to unravel complex multi-directional interactions. Other than classical correlative approaches, our findings are constrained to a few meaningful set of relations which can be powerful insights for the evaluation of terrestrial ecosystem models.
Uncertainty quantification of flood mitigation predictions and implications for interventions
Authors: Berends Koen D. , Straatsma Menno W. , Warmink Jord J. , Hulscher Suzanne J. M. H.
Published: August 2019

Abstract. Reduction of water levels during river floods is key in preventing damage and loss of life.
Computer models are used to design ways to achieve this and assist in the decision-making process.
However, the predictions of computer models are inherently uncertain, and it is currently unknown to what extent that uncertainty affects predictions of the effect of flood mitigation strategies.
In this study, we quantify the uncertainty of flood mitigation interventions on the Dutch River Waal, based on 39 different sources of uncertainty and 12 intervention designs.
The aim of each intervention is to reduce flood water levels.
Our objective is to investigate the uncertainty of model predictions of intervention effect and to explore relationships that may aid in decision-making.
We identified the relative uncertainty, defined as the ratio between the confidence interval and the expected effect, as a useful metric to compare uncertainty between different interventions.
Using this metric, we show that intervention effect uncertainty behaves like a traditional backwater curve with an approximately constant relative uncertainty value.
In general, we observe that uncertainty scales with effect: high flood level decreases have high uncertainty, and, conversely, small effects are accompanied by small uncertainties.
However, different interventions with the same expected effect do not necessarily have the same uncertainty.
For example, our results show that the large-scale but relatively ineffective intervention of floodplain smoothing by removing vegetation has much higher uncertainty compared to alternative options.
Finally, we show how a level of acceptable uncertainty can be defined and how this can affect the design of interventions.
In general, we conclude that the uncertainty of model predictions is not large enough to invalidate model-based intervention design, nor small enough to neglect altogether.
Instead, uncertainty information is valuable in the selection of alternative interventions.
Integrated modeling in the UK: Practical usability of integrated models
Authors: Simmonds David
Published: August 2019

This short paper reviews the range of planning issues that are currently being addressed in Britain and considers how the nature of these issues and the ways in which choices are being assessed impact the modeling approaches being adopted, in particular, by the author’s own consultancy practice. The paper briefly outlines current developments in governance and analytical requirements; implications of trends toward more detailed (and more time-consuming) transport modeling; and the role of microsimulation in both research and planning practice. It concludes primarily that the practicality of model operation, particularly in terms of model run times, is of critical importance, and in many cases, determines whether major planning decisions are made on the basis of formal analysis rooted, albeit indirectly, in research, or without such a basis at all. A secondary conclusion relates to the possible use of output from detailed microsimulation models as a basis for calibrating aggregate models.
Effect of Educational Strategies on the Sleep Quality of People with Diabetes: Randomized Clinical Trial
Authors: Pereira Flávia Helena , Trevisan Danilo Donizetti , Santos Lourenço Daniela , da Silva Juliana Bastoni , Lima Maria Helena Melo
Published: August 2019

Objective: To evaluate the effect of educational strategies on sleep quality and its relation to diabetes-related distress and glycemic control in people with type 2 diabetes mellitus (DM2). Materials and methods: Randomized clinical trial involving two groups. Group 1 (G1, n = 45) received verbal guidance and leaflets on sleep hygiene strategies and group 2 (G2, n = 46) received usual health care guidelines on self-care with the feet. Sleep was assessed by the Pittsburgh Sleep Quality Inventory and diabetes-related distress by the Diabetes Distress Scale. Linear mixed-effects models and linear regression model were used for the statistical analysis. Results: At the end of the follow-up, sleep quality improvement (p = 0.02) was verified in G1. Low diabetes-related distress score (p = 0.03), being male (p = 0.02), belonging to G1 (p = 0.002), and age (p = 0.04) contributed to better sleep quality. Conclusion: Educational guidelines on sleep hygiene in patients with DM2 were effective in improving sleep quality, measured by the PSQI instrument and emotional stress related to diabetes as assessed by the Diabetes Distress Scale.
The Attitudes of Elderly Women Regarding the Expression of Their Sexuality
Authors: da Silva Francielle Garcia , Pelzer Marlene Teda , Neutzling Bruna Ruoso da Silva
Published: August 2019

Objective: To identify the attitudes elderly women have with respect to their sexuality. Material and method: Exploratory, descriptive study of qualitative approach conducted during the second semester of 2018, with the participation of 19 elderly women, between 60 and 69 years of age, members of two groups for the elderly located in the city of Rio Grande-RS, Brazil. Data was collected through individual, semistructured interview and audio recorded. Thereafter, the data were subjected to the Bardin thematic analysis. Results: Upon analyzing the data, four categories emerged: Change in the expression of sexuality after 60 years of age; feelings derived from talking about sexuality; with whom do they talk about sexuality; importance of affective relationships for the elderly. The elderly women show favorable attitudes regarding their sexuality and noted no significant changes in the expression of sexuality after turning 60 years of age. However, they were embarrassed to talk about this matter because they had received a repressive education. In addition, they sought friends to address any questions. Conclusion: Affective, loving, and sexual relation are extremely important, given that it promotes physical and mental wellbeing, generates feelings of joy and happiness, which provides more vitality and pleasure in living to the elderly women.
Quality of Life of Patients with Advanced Cancer in Palliative Therapy and in Palliative Care
Authors: Silva Leonel dos Santos , Lenhani Bruna Eloise , Tomim Dabna Hellen , Bittencourt Guimarães Paulo Ricardo , Kalinke Luciana Puchalski
Published: August 2019

Objective: To assess the quality of life of patients with advanced cancer in palliative therapy and in palliative care. Materials and Methods: Quantitative, observational, cross-sectional, and analytic study conducted in a teaching hospital in Paraná, Brazil, from January to June 2018, with 126 patients: 107 in palliative therapy; 19 in exclusive palliative care. The questionnaires for data collection were: Quality of Life Questionnaire-Core 15-Palliative, Functional Assessment of Chronic Illness Therapy-Palliative Care 14, and Edmonton Symptom Assessment System. The Spearman non-parametric coefficient test was used for the analysis. Results: The overall quality of life in palliative therapy and in palliative care was, respectively, 71.54/59.65; when correlating the total score of quality of life of the Quality of Life Questionnaire-Core 15-Palliative with the Functional Assessment of Chronic Illness Therapy-Palliative Care 14 (p = 0.001), and the Edmonton Symptom Assessment System (p = 0.001), significant difference of better quality of life was observed in the palliative therapy. Conclusion: Patients in palliative therapy have good overall quality of life, while the palliative care group reports regular quality of life. The symptoms were milder in the palliative therapy and more intense and with greater significance in palliative care; hence, knowing the compromise of quality of life will help professionals in planning interventions with transdisciplinary approach for patients and for their families.
Strategies by Educators within the School Setting to Prevent and Cope with the Experience of Domestic Violence by Adolescents
Authors: Cordeiro Kátia Cordélia Cunha , Gomes Nadirlene Pereira , Estrela Fernanda Matheus , da Silva Andrey Ferreira , Magalhães Júlia Renata Fernandes , Lírio Josinete Gonçalves dos Santos
Published: August 2019

Objective: This work sought to identify, in the discourse of educators, which strategies they can implement, within the school setting, to prevent and cope with the experience of domestic violence by adolescents. Method: Qualitative study based on the critical-liberating perspective of Paulo Freire, conducted with 20 educators from a public school in Salvador, Bahia, Brazil. To collect the data, the interview was used and, to systematize them, the discourse of the collective subject. Results: The discourses reveal the following coping strategies of domestic violence experienced by adolescents: place into action the Guardianship Council; create reception and listening spaces for students; strengthen bonds between the school and family; promote educational actions, and articulate knowledge. Conclusions: The study provides paths to prevent and confront family abuses that compromise the physical and mental health of children and adolescents, with impact upon school performance. The study suggests the contribution of primary health care through actions linked to the Health in School Program and the insertion of the theme of violence in the different university careers, given that caring for children and adolescents and defending them constitutes an obligation for all citizens.
The analysis of nanomechanical properties of Candida spp. by atomic force microscopy (AFM) method
Authors: Tokarska-Rodak Małgorzata , Czernik Sławomir , Chwedczuk Marta , Plewik Dorota , Grudniewski Tomasz , Pawłowicz-Sosnowska Ewa Teresa
Published: August 2019

The aim of the study was to analyze the selected nanomechanical properties of Candida spp: Candida albicans (standard strain ATCC 10231), Candida albicans (clinical strain, cultured from an oral swab), Candida lipolytica (clinical strain, cultured from a nosal swab) in atomic force microscopy (AFM).
The culture Candida spp. was performed of Tryptone Soya Broth (BioMaxima). The topography and sample properties were analysed in AFM (Ntegra Spectra C from NT) and the results were carried out using NOVA 1.1.0.1824 software.
C. albicans ATCC 10231 cells were significantly higher 1.81 μm (p = 0.001) from clinical strains: C. albicans (1.30 μm) and C. lipolytica (1.23 μm). C. albicans ATCC 10231 cells, and C. albicans cells of the clinical strain were softer, especially in the top parts of cells, than C. lipolytica cells. Adhesion force measured for C. albicans ATCC 10231 was 62.83 nN, and was significantly higher compared to the values obtained for C. albicans (41.93 nN,
p = 0.0002 ) and C. lipolytica (41.78 nN, p = 0.0002 ). The stiffness of the Candida spp. cell surface was comparable and was in the range of 5–6 nA.
The differences in height may result from different conditions in which clinical strains grow. Adhesion force can be helpful in the analysis of the degree of destruction of the cell wall by various substances. The conducted analyses showed morphological differences and the differences in mechanical properties of the researched Candida spp. This data may be important in assessing their susceptibility to the effects of various substances of a lytic nature.
O PENSAMENTO COMPLEXO E AS TEORIAS DAS ORGANIZAÇÕES: REFLEXÕES
Authors: Silva Shirlei Da Conceição Domingos , Ramalho Wanderley
Published: August 2019

É possível mostrar que a ciência administrativa evolui pari passu com desenvolvimento dos paradigmas científicos. Este artigo tem exatamente o objetivo de apresentar uma síntese deste fato. Fundamentalmente, este trabalho explicita como as abordagens da ciência administrativa tiveram por substrato epistemológico os paradigmas clássico, sistêmico e complexo resultantes da evolução do próprio pensamento científico.
GERENCIAMENTO DE PROJETOS EM MICRO E PEQUENAS EMPRESAS: UM ESTUDO DA APLICAÇÃO NO SETOR ALIMENTÍCIO DA CIDADE DE BETIM/MG
Authors: Melo Jaqueline Silva , De Melo Tatiane Lúcia
Published: August 2019

O conjunto de conhecimentos em gerenciamento de projetos pode ser apresentado por uma série de capacitações e competências na área de gestão que são aceitas por projetos de diversas naturezas. Este trabalho buscou investigar a aplicação das práticas do Gerenciamento de Projetos em micro e pequenas empresas da cidade de Betim/MG, do segmento de alimentação, especificamente nos restaurantes da região central da cidade. Seu embasamento teórico buscou dar um suporte conceitual ao tema proposto, bem como contextualizar a realidade das micro e pequenas empresas no Brasil e na cidade alvo deste estudo. Para tal, foi realizada uma pesquisa aplicada, de natureza qualitativa, onde foram utilizados questionários semiestruturados para a coleta de dados. Os resultados da pesquisa demonstraram que o perfil do empreendedor nesse ramo foi predominantemente do sexo masculino, com nível médio de escolaridade e baixo nível de conhecimento e aplicabilidade das práticas de gerenciamento de projetos preconizadas pelo PMI (Project Management Institute). No entanto, a maioria dos entrevistados manifestou interesse em conhecer e aplicar estas práticas. A fim de enriquecer as pesquisas na área, sugerimos a realização de outras investigações mais profundas visando a disseminação e incentivo da aplicação das práticas em Gerenciamento de Projetos nesse nicho de mercado.
§ 172 StGB – eine entbehrliche Strafnorm?
Authors: Zehetgruber Christoph
Published: August 2019

§ 172 StGB stellt das Eingehen einer weiteren Ehe oder Lebenspartnerschaft trotz bereits bestehender, formell gültiger Ehe bzw. Lebenspartnerschaft unter Strafsanktion. Der folgende Beitrag beleuchtet neben dessen Genese und historischer Entwicklung die Problematik des Rechtsguts der Vorschrift sowie das Verhältnis zum zivilrechtlichen Verbot des § 1306 BGB, stellt einige weitere diffizile Fragestellungen im Zusammenhang mit § 172 StGB dar und votiert im Ergebnis für dessen Streichung aus dem Rechtsbestand des deutschen Strafrechts.
Health as an enforceable positive right: the Brazilian Supreme Federal Court’s case law on the access to medicines
Authors: Sarlet Ingo Wolfgang , Figueiredo Mariana Filchtiner
Published: August 2019

This paper aims to present and discuss how the right to health has been considered a fundamental right and duty in the Brazilian constitutional order, in particular as a positive enforceable entitlement. The main purpose is to analyze - in light of the example of medicines - the role of the Brazilian Supreme Federal Court regarding the effectiveness of the right to health, and to present a discussion about proper criteria to justify judicial decisions in this domain.
Assistierter Suizid und der Geist des Katholizismus
Authors: Tiedemann Paul
Published: August 2019

The Christian churches speak out against the legalization of assisted suicide in a variety of documents by using religiously distant and profane arguments that, however, do not stand up to critical scrutiny. This is all the more astounding as the churches have the tradition of Dolorism and thus a body of belief that makes their position on assisted suicide plausible for explicitly religious reasons. It can be surmised that the attempt to veil the actual religious motivation with the help of bad profane arguments is due to the insight that in the secular world it is not promising to use the language of myth.
Challenging the Far-Right in Greece: A Critical Assessment of the Legal Framework
Authors: Alkiviadou Natalie
Published: August 2019

Der vorliegende Beitrag unterzieht die straf- und zivilrechtlichen Mittel einer kritischen Prüfung, die zur Bekämpfung der extremen Rechten in Griechenland zur Verfügung stehen. Dieses Phänomen dominiert der „Völkische Bund Goldene Morgenröte“ (Λαϊκός Σύνδεσμος-Χρυσή Αυγή), eine politische Partei, die gleichzeitig als gegen Immigranten, ethnische Minderheiten, LGBT-Personen und Antifaschisten gerichtete gewalttätige Bewegung in Erscheinung getreten ist.
„Über den Wolken“ – Grenzenlose Freiheit?
Authors: Braum Stefan
Published: August 2019

La Commission européenne a présenté une proposition de règlement « relative aux injonctions européennes de production et de conservation de preuves électroniques en matière pénale ». Il est destiné à répondre aux lacunes alléguées dans l'application de la loi lors de la collecte d'éléments de preuve à partir de données stockées dans le « cloud ». La proposition représente un changement de paradigme puisque le principe de territorialité est remplacé par le principe du marché. Elle implique un changement d'orientation du droit procédural pénal européen, qui passe de la reconnaissance mutuelle et de la coopération judiciaire à l'accès unilatéral de l'exécutif aux éléments de preuve. Cela porte atteinte aux principes de protection des données. Mais au-delà de ses conséquences immédiates, la proposition est avant tout un modèle à long terme pour déformer le système de justice pénale européen dans son ensemble. Elle fait partie d'un réseau européen d'enquête qui échappe largement à un contrôle judiciaire contraignant et efficace.
Sleep and Orofacial Pain: Physiological Interactions and Clinical Management
Authors: Meira E. Cruz Miguel , Sousa Bruno , De Laat Antoon
Published: August 2019

Sleep and pain are both vital functions on which wellbeing, health, and life itself depend. These two complex states interact in several ways serving homeostasis, but they are also regulated by a well-orchestrated, multi-oscillatory mechanism characterizing the Circadian Timing System. This interaction, which benefits critical physiological challenges, is also clinically crucial, as it mutually affects sleep and pain-related disturbances. It impacts pathophysiological pathways and relevant clinical aspects of many disorders. Furthermore, therapeutic success is frequently dependent on the adequate management of this cycle. The relationship of sleep and pain is undoubtedly of major relevance for diagnosis and successful management of various health conditions and disorders.
Complex analysis of total protein content in bread wheat containing GPC-B1 gene from Triticum turgidum SSP. dicoccoides
Authors: Pokhylko S. Yu. , Schwartau S. V. , Pochynok V. V. , Mykhalska L. M. , Dugan O. M. , Morgun B. V.
Published: August 2019

Aim. The purpose of our study was to determine the content of total protein in the F5 generation grains, the carriers of the Gpc-B1 gene from Triticum turgidum ssp. dicoccoides by two methods, which in turn would confirm the successful expression of the Gpc-B1 gene in the genetic environment of bread winter wheat. Methods. Determination of protein content was carried out by Kjeldahl method and by infrared spectrometry (NIR) method. Results. The 44 hybrid lines that are homozygous for the Gpc-B1 gene from T. turgidum ssp. dicoccoides have been analyzed. It has been established that for both methods, the average content of protein in the grain of hybrid lines is 14 % higher in comparison to the original Kuyalnik variety. Particular attention should be paid to the line number 10, 12 and 35 in which the content of protein exceeds 15 % by the method of Kjeldahl. Conclusions. The obtained results indicate that the gene Gpc-B1 from the wild relative in the new genetic environment of the highly productive registered wheat cultivar Kuyalnik has been functioning and has a positive effect on the accumulation of total protein in grains.Keywords: biofortification, protein content, Triticum aestivum, Gpc-B1 gene, Kjeldahl and NIR methods.
Lysenkoizm phenomenon in the genomic era
Authors: Opalko A. I. , Serzhuk O. P.
Published: August 2019

During the round table meetings «Retrospective analysis of the learning efficiency in a genetics (Current Issues and Trends)», devoted to the 80-th anniversary of Yurii Mykolaiovych Mishkurov, a knowledge specialist of didactic problems of genetics at high school, the actual questions about a new round of perigenetics mythicize which are observed in Genomic era were discussed in the form of open discussion. An attempt to reveal the phenomenon of popularity of pseudogenetic and other pseudoscientific theories, which are spread by current television was done, the role of a teacher in forming a scientific picture of the world and in particular manifestation of heredity and variability was appreciated. It is proposed to file a petition for the correction of the curriculum and returning the subject «Applied Genetics and the basics of cytology» to the list of compulsory disciplines of Master’s degree in specialities: 201 — «Agronomy», 202 — «Plants Protection and Quarantine», 203 — «Horticulture and Viticulture», 205 — «Forestry», 206 — «Landscape Gardening» and for restoration in full volume of contact hours (lectures, laboratory and practical classes), term paper, training practice and planning of hours to control self-learning of students. The content of a Bachelor’s degree program on «Genetics» regarding the restoration of training practice and controlling of selflearning of students must be updated.Keywords: Bologna process, teaching of genetics, pseudoscience, heredity and variability, public consciousness.
Expression analisis of the ITSN2 and TKS5 mRNA isoforms in human malignant breast tumors
Authors: Kropyvko S. V. , Tsyba L. O. , Novokhatska O. V. , Syvak L. A. , Tarasenko T. Ye. , Grabovoy A. N. , Rynditch A. V.
Published: August 2019

Aim. Despite the great progress in cancer treating, the breast cancer remains lethal in 15 % cases. Regardless of the many years of research and extensive experience in the treatment of this type of cancer, one of the main problems in diagnosis and therapy is its high clinical and genetic heterogeneity. Thereby the identification of markers for personalized treatment of patients is still an actual issue. Methods. Collection of clinical material, RNA isolation, and expression analysis of ITSN2 and TKS5 isoforms using quantitative real time PCR with fluorescence-labeled probes. Results. We have found that ITSN2-S expression is reliably reduced in HER2/neu-positive tumors with poor prognosis. There were no significant differences in the expression of ITSN2-L and TKS5-L in the analyzed samples. Conclusions. These studies have demonstrated the possible use of ITSN2 short isoform (ITSN2-S) as a prognostic marker for breast cancer. Keywords: breast cancer, ITSN2, TKS5, expression analysis.
Genetic-physiological basis of legume crops resistance to drought stress
Authors: Sichkar V. I. , Pasichnyk S. M.
Published: August 2019

Goal. Identify the effective criteria for drought tolerances of leguminous crops, based on their basis evaluate the recommendation for cultivating soybean cultivars and discuss the mechanisms of resistance on the organism and molecular levels. Methods. The collection and breeding genotypes of soybean, chickpea and pea were grown in field and laboratory experiments as well as cultivars included in the State register of plant varieties suitable for dissemination in Ukraine. The reaction of soybean plants on the effect of water stress was determined in climatic chambers at the temperature of 30–32 °C. Results. The genetic variability in absorption of water by seeds of various soybean cultivars was detected especially at the initial stages of soaking. The tolerance to drought is associated with the level of free proline accumulation and water-keeping proteins in the leaves, the area of the leaf surface, the loss of moisture by the plant for a certain period, the development of the root system. Soybean cultivars Arcadia odesskaya and Hodson distinguished by increased resistance to drought. Conclusions. Tested in the field and laboratory conditions methods for determining resistance to water stress may be recommended for use in breeding research with agricultural crops. Keywords: breeding of leguminous crops, drought tolerance, adaptability to high temperature.
Cell population genetics: emergence, main results and concepts (to the 50th anniversary of the foundation)
Authors: Kunakh V. A.
Published: August 2019

The article briefly reviews the emergence and development in Ukraine of a new scientific field — cell population genetics, which formed the theoretical basis of modern cell technologies. These include, in particular, plant biotechnologies for the improvement, preservation, and accelerated reproduction of unique genotypes in vitro; development of new genotypes (organisms) by the methods of cell and genetic engineering and cellular selection; obtaining biologically active compounds, including recombinant, from biomass of cultured cells and tissues for the needs of medicine, cosmetics and food industry; as well as the methods of cell therapy, including technologies based on the use of stem cells, etc. Cultured cells are widely used as model objects and biological systems for studying the most relevant problems of modern biology: the features of the course, signaling pathways, and mechanisms of cell proliferation, including carcinogenesis and tumor proliferation; dedifferentiation of cells, including their reversion to a pluripotent state; totipotency, pluripotency and omnipotency; regeneration of tissues, separate organs, and whole organisms, etc. The article reviews scientific prerequisites for the development of the new scientific field and presents the main concepts of cell population genetics, which have been mainly developed in the Department of cell population genetics of the Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine. It also presents an analysis of the features of development of the newest research areas of genetics of somatic cells of intact plants and cells in vitro, cell population genetics, genetic foundations of cell selection, cell biology, and biotechnology during the second half of the past and the beginning of this century. Keywords: history of science, cell population genetics, plant tissue and cell culture, cell selection, plant biotechnology.
Influence of presowing irradiation of seeds on the salt tolerance of chickpea
Authors: Jafarov E. S. , Velijanova M. Z. , Orujova J. R.
Published: August 2019

Aim. The aim of the research was to study the influence of salt stress on the growth and development of chickpea, whose seeds were irradiated at different doses before sowing. The physiological state of the investigated plant is evaluated on the basis of the functioning of the antioxidant enzymes of the protection system. Methods. The methods developed by Kumar, Knovles and Nakano, Assad was used to determine the activity of antioxidant enzymes. Results. It is shown that under the conditions of salt stress, antioxidant enzymes function, to some extent, interconnected and coordinated. Conclusions. It is assumed that the survival of chickpea seedlings in salt conditions is ensured by the coordinated work of the antioxidant defense system.Keywords: Cicer arietinum L., presowing seed irradiation, salt stress, antioxidant enzymes.
Study of transgene expression in Triticum aestivum L. after Agrobacterium-mediated in planta transformation
Authors: Zhalii N. A. , Bannikova M. O. , Plugatar M. O. , Velikozhon L. H. , Taranenko A. M. , Morgun B. V.
Published: August 2019

Aim. Detection of sequences of target transgenes nptII and bar in the genome of probable transformants of bread winter wheat Triticum aestivum L. cultivars Zymoiarka and Podolianka obtained as a result of Agrobacterium-mediated transformation in planta and determination of their expression level. Methods. Polymerase chain reaction (PCR) method was used independently and in combination with reverse transcription (RT-PCR), electrophoresis of DNA in agarose gel. Tolerance to the herbicide was evaluated in the physiological test. Results Through PCR analysis, the sequence of nptII transgene was detected in 30 samples of 145 analyzed, the frequency of transformation was 20.7 %. The sequence of the gene bar was observed in 85 experimental plants, and the frequency of transformation was 15.6 %. mRNAs of both transgenes were detected, indicating their transcriptional activity and stable expression. Conclusions PCR analysis allowed to detect nptII transgenic signal in 20.7 % of plants, while the presence of the bar gene was detected in 15.6 % of cases, indicating a higher efficiency of this genetic construct. The transcription is shown in all the specimens studied for both transgene. According to the results of the physiological test, 25 % of plants containing the gene bar showed resistance to the Basta® herbicide.Keywords: genetically modified organisms, transgenic plants, biotechnological cultures, bread winter wheat, genetic engineering.
Determination of the united quality latent index of adaptability (UQLIA) and contribution of some environmental parameters to it for Deschampsia antarctica populations, Galindez island (Maritime Antarctic) season 2017/2018
Authors: Parnikoza I. Y. , Miryuta N. Y. , Ivanets V. Y. , Dykyi E. O.
Published: August 2019

The purpose of our work has been to determine the indicator of complex adaptability — the United Quality Latent Index of Adaptability (UQLIA) for the experimental populations of Deschampsia antarctica É. Desv. and study the contribution to it of some environmental factors such as the near soil surface temperature and organogens content. Materials and methods. The determination of UQLIA was based on a pairwise comparison of the differences between investigated parameters of populations by mathematical regression techniques. The soil surface temperature was measured by loggers installed near plants in each locus during April 2017 – April 2018. Results and conclusions. Temperature fluctuations were described during December 2017 – February 2018 for twelve experimental populations of D. antarctica and one control fragment of moss turf subformation from Galindez Island. Significant variations in average daily near surface temperature were observed during the study period between populations, especially in December and January. The UQLIA of D. antarctica for this season was calculated on the basis of the projective cover, biometric indices of generative plants and the content of protective and reserve proteins in seeds for the eleven populations. The values of the United Soil Surface Temperature Influence Index (UTII) for the season summer months and the United Organogens Content in Soil Influence Index (UOCSII) have been calculated for the individual parameters of D. antarctica plants adaptability. The reliable contribution of UTII to ULIA has been shown for December and January, at the moment of the greatest variation of soil surface temperature. UOCSII provided a reliable contribution to the ULIA only in the amount of UTII. Keywords: Deschampsia antarctica, United Quality Latent Index of Adaptability (UQLIA), contribution of soil surface temperature and organogens content to complex adaptability.
The relationship of Wolbachia infection and different phenotypes in the Drosophila melanogaster natural populations from radioactively polluted and clear areas in Ukraine
Authors: Gora N. V. , Serga S. V. , Maistrenko O. M. , Protsenko O. V. , Kozeretska I. A.
Published: August 2019

Aim. The study was performed to investigate the relationship between Wolbachia infection and phenotypes that distinct from wild-type of Drosophila melanogaster from different localities in Ukraine including those from Chornobyl Exclusion Zone during 2013–2014. Methods. We have established isofemale lines from populations: Uman’, Inkerman, Odesa, Varva, Kyiv, Drogobych, Yaniv, Poliske, Chornobyl, and Chornobyl Nuclear Power Plant (NPP). The ambient radiation (µSv/h) was measured in the sample sites. The flies were reared in the laboratory through two generations. We carried out the observation of F2 flies for visibly detectable phenotypes. According to whether the trait was inherited, observations were separated into three categories: with deviations of posterior cross-vein (C2) (incomplete penetrance), visible phenotypic changes (non-inherited) and mutations (inherited). Polymerase chain reaction (PCR) with primers specific to the 16S rRNA and Wolbachia surface protein (wsp) genes were used to determine infection presence in isofemale lines of the flies established for each population. Results. Examination of different phenotypes indicates that the highest mutation rate (but not C2 and not inherited changes) is in populations from Chornobyl Exclusion Zone and, therefore, connection with ambient radiation was detected (p = 0.0241). Generalized mixed linear regression has shown evidence that the presence of phenotypes with defects of C2 vein varies with endosymbiont infection presence (p = 0.03473) in the populations from radioactively polluted areas. Conclusion. Wolbachia is not related to occurring phenotypes neither with phenotypic changes nor with mutations, at least in surveyed populations. However, C2 defected phenotypes relates to the bacterial presence in populations from the contaminated area. Nonetheless, the origin of this relationship is unknown and the mechanisms of such a connection require further research. Keywords: Drosophila melanogaster, Wolbachia, endosymbiont, ambient radiation, mutation, phenotypic change, posterior cross-vein.
The Effect of Age on Outcome in Excision of Chronic Osteomyelitis with Free Muscle Flap Reconstruction
Authors: Kendall John Victor , McNally Martin , Taylor Christopher , Ferguson Jamie , Galitzine Svetlana , Critchley Paul , Giele Henk , Ramsden Alexander John
Published: August 2019

Abstract. Introduction: Curative surgical treatment of chronic osteomyelitis often requires free tissue transfer if there is significant soft tissue compromise. We investigated whether age influenced outcomes of curative osteomyelitis excision in those patients requiring free muscle flap soft tissue reconstruction.Methods: We assessed ninety-five consecutive patients treated with excision of chronic osteomyelitis, skeletal stabilisation/reconstruction and free muscle transfer between 2006 and 2012. We compared outcomes of those aged ≥60 years (n=23) with those <60 years old (n=72).Results: Groups were similar with regard to Cierny and Mader anatomic type and physiological host classification. Length of procedure and length of hospital stay were similar for both groups. There was a greater proportion of ASA grade III patients in the older cohort. Infection recurrence occurred in one of the older cohort (4.3%) and in seven patients in the younger cohort (9.9%) at a mean follow-up of 42 months (range 11-131 months), this was not statistically significant (p=0.27874). There were five free flap losses (6.9%) in the younger group and none in the older group. A greater proportion of patients from the younger cohort required further unplanned surgery (28%) compared to the older group (4.3%), which was statistically significant (p=0.01174). Seven patients (7.4%) had serious medical complications - five of whom were in the younger cohort, including one mortality.Conclusions: Both the young and old can enjoy satisfactory outcomes from surgical resection of chronic osteomyelitis with simultaneous orthoplastic reconstruction including free tissue transfer. Age alone should not be a barrier to potentially curative surgical treatment.