bluuebunny/crossref_metadata_2025 huggingface dataset:
Genomic and Comparative Transcriptomic Analyses Reveal Key Genes Associated with the Biosynthesis Regulation of Okaramine B in Penicillium daleae NBP-49626
Authors: Wang Yueying , Chen Ling , Fang Wei , Zeng Zhen , Wu Zhaoyuan , Liu Fang , Liu Xiaoyan , Gong Yan , Zhu Lei , Wang Kaimei
Published: February 2024

Restricted production of fungal secondary metabolites hinders the ability to conduct comprehensive research and development of novel biopesticides. Okaramine B from Penicillium demonstrates remarkable insecticidal efficacy; however, its biosynthetic yield is low, and its regulatory mechanism remains unknown. The present study found that the yield difference was influenced by fermentation modes in okaramine-producing strains and performed genomic and comparative transcriptome analysis of P. daleae strain NBP-49626, which exhibits significant features. The NBP-49626 genome is 37.4 Mb, and it encodes 10,131 protein-encoding genes. Up to 5097 differentially expressed genes (DEGs) were identified during the submerged and semi-solid fermentation processes. The oka gene cluster, lacking regulatory and transport genes, displayed distinct transcriptional patterns in response to the fermentation modes and yield of Okaramine B. Although transcription trends of most known global regulatory genes are inconsistent with those of oka, this study identified five potential regulatory genes, including two novel Zn(II)2Cys6 transcription factors, Reg2 and Reg19. A significant correlation was also observed between tryptophan metabolism and Okaramine B yields. In addition, several transporter genes were identified as DEGs. These results were confirmed using real-time quantitative PCR. This study provides comprehensive information regarding the regulatory mechanism of Okaramine B biosynthesis in Penicillium and is critical to the further yield improvement for the development of insecticides.
Mesocellular Silica Foam as Immobilization Carrier for Production of Statin Precursors
Authors: Skendrović Dino , Primožič Mateja , Rezić Tonči , Vrsalović Presečki Ana
Published: February 2024

The employment of 2-deoxyribose-5-phosphate aldolase (DERA) stands as a prevalent biocatalytic route for synthesizing statin side chains. The main problem with this pathway is the low stability of the enzyme. In this study, mesocellular silica foam (MCF) with different pore sizes was used as a carrier for the covalent immobilization of DERA. Different functionalizing and activating agents were tested and kinetic modeling was subsequently performed. The use of succinic anhydride as an activating agent resulted in an enzyme hyperactivation of approx. 140%, and the stability almost doubled compared to that of the free enzyme. It was also shown that the pore size of MCF has a decisive influence on the stability of the DERA enzyme.
Transcriptomic and Metabolic Profiling Reveal the Mechanism of Ovule Development in Castanea mollissima
Authors: Cui Yanhong , Ji Xingzhou , Zhang Yu , Liu Yang , Bai Qian , Su Shuchai
Published: February 2024

Ovule abortion, which is the main cause of empty burs in the Chinese chestnut, affects the formation of embryos and further reduces yield; therefore, it is important to study the mechanism of ovule abortion. In this study, we analyzed the transcriptomic and metabolomic data of ovules at critical developmental stages to explore the key regulatory networks affecting ovule development. The metabolites were enriched mainly in pathways involved in phytohormone signaling, energy metabolism, and amino acid synthesis in the endoplasmic reticulum. Analysis of the differentially expressed genes (DEGs) revealed that the HSP genes were significantly down-regulated during fertilization, indicating that this process is extremely sensitive to temperature. The hormone and sucrose contents of ovules before and after fertilization and of fertile and abortive ovules at different developmental stages showed significant differences, and it is hypothesized that that abnormal temperature may disrupt hormone synthesis, affecting the synthesis and catabolism of sucrose and ultimately resulting in the abortive development of Chinese chestnut ovules. At the pollination and fertilization stage of chestnuts, spraying with ethylene, ACC, and AIB significantly increased the number of developing fruit in each prickly pod compared to CK (water) treatment. These results indicated that both ethylene and ACC increased the rate of ovule development. This study provides an important theoretical molecular basis for the subsequent regulation of ovule development and nut yield in the Chinese chestnut.
The Role of the Gut Microbiome and Microbial Dysbiosis in Common Skin Diseases
Authors: Ryguła Izabella , Pikiewicz Wojciech , Grabarek Beniamin Oskar , Wójcik Michał , Kaminiów Konrad
Published: February 2024

Dermatoses are an increasingly common problem, particularly in developed countries. The causes of this phenomenon include genetic factors and environmental elements. More and more scientific reports suggest that the gut microbiome, more specifically its dysbiosis, also plays an important role in the induction and progression of diseases, including dermatological diseases. The gut microbiome is recognised as the largest endocrine organ, and has a key function in maintaining human homeostasis. In this review, the authors will take a close look at the link between the gut–skin axis and the pathogenesis of dermatoses such as atopic dermatitis, psoriasis, alopecia areata, and acne. The authors will also focus on the role of probiotics in remodelling the microbiome and the alleviation of dermatoses.
Autonomous Cycles of Data Analysis Tasks for the Automation of the Production Chain of MSMEs for the Agroindustrial Sector
Authors: Fuentes Jairo , Aguilar Jose , Montoya Edwin , Pinto Ángel
Published: February 2024

In this paper, we propose autonomous cycles of data analysis tasks for the automation of the production chains aimed to improve the productivity of Micro, Small and Medium Enterprises (MSMEs) in the context of agroindustry. In the autonomous cycles of data analysis tasks, each task interacts with the others and has different functions, in order to reach the goal of the cycle. In this article, we identify three industrial-automation processes within the production chain, in which autonomous cycles can be applied. The first cycle is responsible to identify the type of input to be transformed—such as quantity, quality, time, and cost—based on information from the organization and its context. The second cycle selects the technological level used in the raw-material transformation, characterizing the platform of plant processing. The last cycle identifies the level of specialization of the generated product, such as the quality and value of the product. Finally, we apply the first autonomous cycle to define the type of input to be transformed in a coffee factory.
Modeling of Impurities Evaporation Reaction Order in Aluminum Alloys by the Parametric Fitting of the Logistic Function
Authors: Mitrašinović Aleksandar M. , Nešković Jasmina , Polavder Svetlana , Petković Sandra , Praštalo Željko , Labus Nebojša , Radosavljević Milinko
Published: February 2024

Advancements in computer capabilities enable predicting process outcomes that earlier could only be assessed after post-process analyses. In aerospace and automotive industries it is important to predict parts properties before their formation from liquid alloys. In this work, the logistic function was used to predict the evaporation rates of the most detrimental impurities, if the temperature of the liquid aluminum alloy was known. Then, parameters of the logistic function were used to determine the transition points where the reaction order was changing. Samples were heated to 610 °C, 660 °C, 710 °C, and 760 °C for one hour, after which the chemical analyses were performed and evaporation rates were calculated for Cd, Hg, Pb and Zn elements. The pressure inside the encapsulated area was maintained at 0.97 kPa. Whereas parameters that define the evaporation rate increase with the temperature increase, the maximum evaporation rates were deduced from the experimental data and fitted into the logistic function. The elemental evaporation in liquid-aluminum alloys is the best defined by the logistic function, since transitions from the first to zero-order-governed evaporation reactions have nonsymmetrical evaporation rate slopes between the lowest and the highest evaporation rate point.
Enhanced Microstructure and Wear Resistance of Ti–6Al–4V Alloy with Vanadium Carbide Coating via Directed Energy Deposition
Authors: Ko Ui Jun , Jung Ju Hyeong , Kang Jung Hyun , Choi Kyunsuk , Kim Jeoung Han
Published: February 2024

Ti–6Al–4V alloys are known for their suboptimal tribological properties and are often challenged by durability issues under severe wear conditions. This study was conducted to enhance the alloy’s wear resistance by forming a hardened surface layer. Utilizing directed energy deposition (DED) additive manufacturing with a diode laser, vanadium carbide particles were successfully integrated onto a Ti–6Al–4V substrate. This approach deviates from traditional surface enhancement techniques like surface hardening and cladding, as it employs DED additive manufacturing under parameters akin to those used in standard Ti–6Al–4V production. The formed vanadium carbide layer achieved a remarkable thickness of over 400 µm and a Vickers hardness surpassing 1500 HV. Pin-on-disk test results further corroborated the enhanced surface wear properties of the Ti–6Al–4V alloy following the additive-manufacturing process. These findings suggest that employing vanadium carbide additive manufacturing, under conditions similar to the conventional DED process with a diode laser, significantly improves the surface wear properties of Ti–6Al–4V in metal 3D-printing applications.
Formation, Stability, and Crystallinity of Various Tricalcium Aluminate Polymorphs
Authors: Ravaszová Simona , Dvořák Karel , Boháč Martin , Všianský Dalibor , Jančíková Andrea
Published: February 2024

Tricalcium aluminate is an important phase of Portland clinker. In this paper, three polymorphs of C3A were prepared by means of the solid-state synthesis method using intensive milling of the raw material mixture which was doped with various amounts of Na2O and sintered at a temperature of 1300 °C for 2 h. The final products were evaluated through X-ray diffraction using Rietveld analysis. The effect of the Na dopant content on the change in the crystalline structure of tricalcium aluminate was studied. It was proven that the given preparation procedure, which differed from other studies, was close to the real conditions of the formation of Portland clinker, and it was possible to prepare a mixture of different polymorphs of calcium aluminate. Fundamental changes in the crystal structure occurred in the range of 3–4% Na, when the cubic structure changes to orthorhombic. At a dosage of Na dopant above 4%, the orthorhombic structure changes to a monoclinic structure. There are no clearly defined boundaries for the existence of individual C3A phases; these phases arise at the same time and overlap each other in the areas of their formation at different Na doses.
Multifunctionality Analysis of Structural Supercapacitors— A Review
Authors: Zschiebsch Willi , Sturm Yannick , Kucher Michael , Hedayati Davood Peyrow , Behnisch Thomas , Modler Niels , Böhm Robert
Published: February 2024

Structural supercapacitors (SSCs) are multifunctional energy storage composites (MESCs) that combine the mechanical properties of fiber-reinforced polymers and the electrochemical performance of supercapacitors to reduce the overall mass in lightweight applications with electrical energy consumption. These novel MESCs have huge potentials, and their properties have improved dramatically since their introduction in the early 2000’s. However, the current properties of SSCs are not sufficient for complete energy supply of electrically driven devices. To overcome this drawback, the aim of the current study is to identify key areas for enhancement of the multifunctional performance of SSCs. Critical modification paths for the SSC constituents are systematically analyzed. Special focus is given to the improvement of carbon fiber-based electrodes, the selection of structural electrolytes and the implementation of separators for the development of more efficient SSCs. Finally, current SSCs are compared in terms of their multifunctionality including material combinations and modifications.
Effect of Sintering Temperature on Microstructure Characteristics of Porous NiTi Alloy Fabricated via Elemental Powder Sintering
Authors: Miao Tianhu , Zhan Sha , Chen Xiaojuan , Hu Li
Published: February 2024

To investigate the effect of the sintering temperature on the microstructure characteristics of porous NiTi alloys, two types of porous NiTi alloys with equal atomic ratios were fabricated via elemental powder sintering at 950 °C and 1000 °C. Afterwards, optical microscopy (OM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were collectively applied to investigate the pore characteristics and microstructure of the fabricated porous NiTi alloy. The results show that when the sintering temperature increases from 950 °C to 1000 °C, the average pore size increases from 36.00 μm to 181.65 μm, owing to the integration of these newly formed small pores into these pre-existing large-sized pores. The measured density increases from 2.556 g/cm3 to 3.030 g/cm3, while the porosity decreases from 60.4% to 51.8%. This is due to the occurrence of shrinkage after the sufficient diffusion of atoms. Furthermore, the characterization results confirm that a change in the sintering temperature would not change the phase types within a porous NiTi alloy; namely, the matrix consists primarily of B2 NiTi, with a significant amount of Ni4Ti3 precipitates and a small amount of Ni3Ti precipitates and Ti2Ni precipitates. However, as the sintering temperature increases, the number of Ni4Ti3 precipitates decreases significantly. The formation of a Ni4Ti3 phase in the present study is closely related to the enrichment of Ni content in the matrix owing to the diffusion rate difference between Ni atoms and Ti atoms and the absence of a transient liquid phase (TLP) during the sintering process owing to the relatively low sintering temperature (lower than the eutectic temperature). Moreover, the increasing sintering temperature speeds up the atom diffusion, which contributes to a reduction in the enrichment of Ni as well as the number of formed Ni4Ti3 precipitates.
Challenges and Future Recommendations for Lightning Strike Damage Assessments of Composites: Laboratory Testing and Predictive Modeling
Authors: Wang Yeqing , Fan Yin , Zhupanska Olesya I.
Published: February 2024

Lightning strike events pose significant challenges to the structural integrity and performance of composite materials, particularly in aerospace, wind turbine blade, and infrastructure applications. Through a meticulous examination of the state-of-the-art methodologies of laboratory testing and damage predictive modeling, this review elucidates the role of simulated lightning strike tests in providing inputs required for damage modeling and experimental data for model validations. In addition, this review provides a holistic understanding of what is there, what are current issues, and what is still missing in both lightning strike testing and modeling to enable a robust and high-fidelity predictive capability, and challenges and future recommendations are also presented. The insights gleaned from this review are poised to catalyze advancements in the safety, reliability, and durability of composite materials under lightning strike conditions, as well as to facilitate the development of innovative lightning damage mitigation strategies.
A Generic Model to Assess the Efficiency Analysis of Cellular Foams
Authors: Avalle Massimiliano
Published: February 2024

One of the main types of uses of cellular materials is for energy absorption and dissipation in applications, such as safety and packaging, to protect people and goods during impact situations. In such cases, the use of cellular materials is justified by their capacity to largely deform under limited loads. This is often achieved, alone or within energy absorbing structures, with the additional advantage of cheap components that are relatively simple to manufacture and assemble. As in most engineering applications, weight reduction is sought after and, as in the case of other materials, this objective can be attained by optimizing the use of the material. Optimization of a cellular material for energy absorption means obtaining an optimal mechanical characteristic that can be obtained by properly designing it in terms of the type of base material and cell properties. Cell properties are mainly related to density and their optimal selection can be made by means of energy criteria. The aim of the present paper is to discuss such optimality criteria based on what are termed efficiency diagrams to produce an effective design tool. Additionally, based on empiric observations on the behavior of several classes of polymeric foams, a simplified selection method is proposed to hasten the selection criteria.
Self-Healing of Cracks in Cementitious Materials as a Method of Improving the Durability of Pre-Stressed Concrete Railway Sleepers
Authors: Dudek Marta , Stryszewska Teresa
Published: February 2024

The article presents research results regarding the possibility of modifying pre-stressed concrete railway sleepers to improve their durability. The cracks that appear in these elements are one of the reasons for shortening the period of safe use. They do not have a significant impact on the load-bearing capacity of these elements, but on their durability. The resulting scratches become an easy way for the external environment to migrate inside the element, including the reinforcement area. Despite efforts to eliminate the possibility of cracking, this phenomenon still occurs in railway sleepers. In order to reduce the negative effects of cracking the cement matrix, a technology for modifying a prefabricated concrete element with resin-filled tubes towards its autonomous self-healing was developed and tested. The tests were divided into three stages, including laboratory tests carried out on cement mortar beams, semi-technical tests carried out on reinforced concrete beams, and industrial tests carried out on pre-stressed concrete and prefabricated railway sleepers. All research conducted on a laboratory and semi-technical scale, preceding the target stage, was intended to ultimately enable the development of tube application technology on an industrial scale while verifying the effectiveness of self-healing at the laboratory level. The use of self-healing cementitious materials potentially reduces the negative effects of cracking railway sleepers, as shown by observations conducted during the research.
Autologous Tooth Granulometry and Specific Surface Area with Three Grinding Methods: An In Vitro Study
Authors: Ribes Berta Lavarias , Fernández-Baca Ignacio , Gil Mur Javier , López-Malla Matute Joaquin , Aragoneses Lamas Juan Manuel
Published: February 2024

A postextraction socket becomes a clinical challenge due to the fact that a series of changes associated with bone remodelling and resorption of the socket that occur after extraction, which limits the aesthetic and functional prognosis of implant-supported rehabilitations. It has been studied that the use of the autologous tooth-derived graft (ATDG) has regenerative properties and could therefore be useful for solving this type of problem. There is no consensus in the scientific literature on a standardized protocol for the use of the autologous tooth. Therefore, the aim of the present study was to evaluate the most relevant parameters to achieve the best properties of ground ATDG using three methods, namely Gouge forceps, electric grinder, and manual, that made up the study group (SG) and compared with the control group (CG) consisting of Bio-Oss®. The sample obtained by the electric grinder had the highest value of specific surface area (2.4025 ± 0.0218 m2/g), while the particle size as average diameter (751.9 µm) was the lowest and most homogeneous of the three groups. Therefore, the electric grinder allowed for obtaining ATDG with more regenerative properties due to its specific surface-area value and particle size in accordance with the xenograft with the greatest bibliographical support (Bio-Oss®). The higher specific surface increases the reaction with the physiological media, producing faster biological mechanisms.
Feasibility of Using New Sustainable Mineral Additions for the Manufacture of Eco-Cements
Authors: Moreno S. , Rosales M. , Rosales J. , Agrela F. , Díaz-López J. L.
Published: February 2024

Due to a continuously developing population, our consumption of one of the most widely used building materials, concrete, has increased. The production of concrete involves the use of cement whose production is one of the main sources of CO2 emissions; therefore, a challenge for today’s society is to move towards a circular economy and develop building materials with a reduced environmental footprint. This study evaluates the possibility of using new sustainable supplementary cementitious materials (SCMs) from waste such as recycled concrete aggregates (RCAs) and mixed recycled aggregates (MRAs) from construction and demolition waste, as well as bottom ash from olive biomass (BBA-OL) and eucalyptus biomass ash (BBA-EU) derived from the production of electricity. A micronisation pre-treatment was carried out by mechanical methods to achieve a suitable fineness and increase the SCMs’ specific surface area. Subsequently, an advanced characterisation of the new SCMs was carried out, and the acquired properties of the new cements manufactured with 25% cement substitution in the new SCMs were analysed in terms of pozzolanicity, mechanical behaviour, expansion and setting time tests. The results obtained demonstrate the feasibility of using these materials, which present a composition with potentially reactive hydraulic or pozzolanic elements, as well as the physical properties (fineness and grain size) that are ideal for SCMs. This implies the development of new eco-cements with suitable properties for possible use in the construction industry while reducing CO2 emissions and the industry’s carbon footprint.
Study on Seepage Characteristics of Grouting Slurry for Water-Absorbing Mudstone with Rough Fissure
Authors: Chen Zhe , Zhou Yue-Jin , Zhang Lei-Ming , Xu Yu-Nong
Published: February 2024

Based on the computed tomography scanning, which abbreviation is CT scanning, and fractal theory, geometric parameters of mudstone fissures are obtained. The physical model of a single fissured channel is obtained in combination with Barton standard curves and 3D printing technology, and similar materials of mudstone are developed based on the water absorption of natural mudstone to prepare single fissured water-absorbing grouting test blocks with different roughness levels for the grouting simulation testing. By analyzing the viscosity change characteristics of grouting slurry before and after grouting, the seepage characteristics of the grouting slurry in the rough fissures of the water-absorbing mudstone are revealed. The results show that when the roughness is small, the grouting slurry will have an obvious water loss effect after passing through mudstone fissures. However, with the flow of the slurry, the water loss effect of the subsequent grouting slurry will be weakened. For fissures with high roughness, the water absorption properties of the rough surfaces and the walls of the mudstone fissures work together, leading to the sedimentation and blockage of the fissure channels, thereby hindering the flow of slurry.
Genesis of Pb–Zn Mineralization in the Pulang Cu Polymetallic Deposit in Yunnan Province, China: Insights from Analyses of Geology, Fluid Inclusions and C–H–O–S Isotopes
Authors: Xu Jingwei , Zhao Xiaoyu , Deng Mingguo , Li Wenchang , Su Yan
Published: February 2024

The Pulang super-large porphyry Cu polymetallic deposit, located in the Sanjiang area of Yunnan Province, is one of the largest Cu deposits in China. This deposit hosts Cu resources of ~5 × 106 t and other ore-forming elements, such as Mo, Au, Ag, Pb, Zn, Pt and Pd. Recently, obvious hydrothermal vein-type Pb–Zn mineralization, with a Pb + Zn resource of ~0.4 × 106 t, has been detected in the North Ore Section of the deposit. However, the genesis of these Pb–Zn ore bodies, especially their relationship to the major Cu ore bodies in the South Ore Section, remains controversial. We conducted geologic description, fluid inclusion petrography and microthermometry, and C, H, O and S isotope studies to uncover the genesis of Pb–Zn vein-type mineralization in North Pulang. As a result, three types of Pb–Zn veins were identified: a quartz–pyrrhotite–chalcopyrite–sphalerite–galena vein, a quartz–pyrrhotite–sphalerite–galena vein, and a calcite–quartz–pyrrhotite–galena vein. All fluid inclusions in the quartz from different veins are liquid-rich inclusions, with homogenization temperatures in the range of 184 °C–235 °C and salinities between 10.4 wt.% and 17.8 wt.% NaCl eq., indicating that the Pb–Zn ore-forming fluid was a single-phase fluid with a low temperature and low-to-medium salinity. Hydrothermal quartz in different stages displays δDwater values ranging from −46.9‰ to −120.0‰ (V-SMOW), and the calculated δ18Owater values range from 2.4‰ to 4.3‰ (V-SMOW), implying that the mineralization fluids likely originated from magma, with a minor involvement of meteoric water. The δ13CCal values (−2.3‰ to −7.9‰ V-PDB) of calcite indicate that C likely originated from a deep-seated source. The δ34S values of chalcopyrite, pyrite, pyrrhotite and sphalerite reveal that S was possibly derived from magmatic rocks. Based on the above data, it is suggested that the Pb–Zn mineralization in North Pulang was the result of the northward migration of ore-forming fluids that originated from South Pulang along the NE-trending structural fractures. A strong water–rock interaction occurred during the migration process. However, the involvement of meteoric water and accompanied cooling of fluids were most likely responsible for the precipitation of galena and sphalerite.
Characterization, Analysis, and Investigation of the Provenance of the Stone Construction Materials of the Vera Cruz Church (Segovia, Spain)
Authors: Sanz-Arauz David , Rodríguez-Escalante María , del Río-Calleja Beatriz , López-Andrés Sol
Published: February 2024

As part of the restoration project of the 13th century Vera Cruz Church (Segovia, Spain), a preliminary study of the stones used in its construction—dolostones of the Montejo de la Vega Fm of Santonian age (Upper Cretaceous)—and the possible location of the quarry of the provenance of these stones was carried out. For this purpose, a comparative analysis was performed between the currently active quarry of La Vera Cruz n° 88 and the historical quarry of Zamarramala. The samples were characterized using a multidisciplinary approach, combining X-ray diffraction (XRD), polarized optical microscopy (POM), and scanning electron microscopy with microanalysis (SEM-EDX). The combined mineralogical, chemical, and textural characterization provided valuable information on historical provenance. The results show a significant correlation between the dolostone samples obtained from the church and both quarries. The integral characterization confirms the suitability of the dolostones identified in the currently active quarry, Vera Cruz n° 88, for the restoration of the Vera Cruz Church, ensuring the use of appropriate materials. This study contributes to the restoration efforts of the Vera Cruz Church by providing a deeper understanding of the historical origins of the building stones.
Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery
Authors: Bilal Muhammad , Degorska Oliwia , Szada Daria , Rybarczyk Agnieszka , Zdarta Agata , Kaplon Michal , Zdarta Jakub , Jesionowski Teofil
Published: February 2024

In the presented study, a variety of hybrid and single nanomaterials of various origins were tested as novel platforms for horseradish peroxidase immobilization. A thorough characterization was performed to establish the suitability of the support materials for immobilization, as well as the activity and stability retention of the biocatalysts, which were analyzed and discussed. The physicochemical characterization of the obtained systems proved successful enzyme deposition on all the presented materials. The immobilization of horseradish peroxidase on all the tested supports occurred with an efficiency above 70%. However, for multi-walled carbon nanotubes and hybrids made of chitosan, magnetic nanoparticles, and selenium ions, it reached up to 90%. For these materials, the immobilization yield exceeded 80%, resulting in high amounts of immobilized enzymes. The produced system showed the same optimal pH and temperature conditions as free enzymes; however, over a wider range of conditions, the immobilized enzymes showed activity of over 50%. Finally, a reusability study and storage stability tests showed that horseradish peroxidase immobilized on a hybrid made of chitosan, magnetic nanoparticles, and selenium ions retained around 80% of its initial activity after 10 repeated catalytic cycles and after 20 days of storage. Of all the tested materials, the most favorable for immobilization was the above-mentioned chitosan-based hybrid material. The selenium additive present in the discussed material gives it supplementary properties that increase the immobilization yield of the enzyme and improve enzyme stability. The obtained results confirm the applicability of these nanomaterials as useful platforms for enzyme immobilization in the contemplation of the structural stability of an enzyme and the high catalytic activity of fabricated biocatalysts.
TLC-Bioautography-Guided Isolation and Assessment of Antibacterial Compounds from Manuka (Leptospermum scoparium) Leaf and Branch Extracts
Authors: Xu Wenliang , Shi Danxia , Chen Kuanmin , Popovich David G.
Published: February 2024

A rapid procedure for the targeted isolation of antibacterial compounds from Manuka (Leptospermum scoparium) leaf and branch extracts was described in this paper. Antibacterial compounds from three different Manuka samples collected from New Zealand and China were compared. The active compounds were targeted by TLC-bioautography against S. aureus and were identified by HR-ESI-MS, and -MS/MS analysis in conjunction with Compound Discoverer 3.3. The major antibacterial component, grandiflorone, was identified, along with 20 β-triketones, flavonoids, and phloroglucinol derivatives. To verify the software identification, grandiflorone underwent purification via column chromatography, and its structure was elucidated through NMR analysis, ultimately confirming its identity as grandiflorone. This study successfully demonstrated that the leaves and branches remaining after Manuka essential oil distillation serve as excellent source for extracting grandiflorone. Additionally, we proposed an improved TLC-bioautography protocol for evaluating the antibacterial efficacy on solid surfaces, which is suitable for both S. aureus and E. coli. The minimum effective dose (MED) of grandiflorone was observed to be 0.29–0.59 μg/cm2 against S. aureus and 2.34–4.68 μg/cm2 against E. coli, respectively. Furthermore, the synthetic plant growth retardant, paclobutrazol, was isolated from the samples obtained in China. It is hypothesized that this compound may disrupt the synthesis pathway of triketones, consequently diminishing the antibacterial efficacy of Chinese Manuka extract in comparison to that of New Zealand.
Multiscale Modeling of Macromolecular Interactions between Tau-Amylin Oligomers and Asymmetric Lipid Nanodomains That Link Alzheimer’s and Diabetic Diseases
Authors: Santos Natalia , Segura Luthary , Lewis Amber , Pham Thuong , Cheng Kwan H.
Published: February 2024

The molecular events of protein misfolding and self-aggregation of tau and amylin are associated with the progression of Alzheimer’s and diabetes, respectively. Recent studies suggest that tau and amylin can form hetero-tau-amylin oligomers. Those hetero-oligomers are more neurotoxic than homo-tau oligomers. So far, the detailed interactions between the hetero-oligomers and the neuronal membrane are unknown. Using multiscale MD simulations, the lipid binding and protein folding behaviors of hetero-oligomers on asymmetric lipid nanodomains or raft membranes were examined. Our raft membranes contain phase-separated phosphatidylcholine (PC), cholesterol, and anionic phosphatidylserine (PS) or ganglioside (GM1) in one leaflet of the lipid bilayer. The hetero-oligomers bound more strongly to the PS and GM1 than other lipids via the hydrophobic and hydrophilic interactions, respectively, in the raft membranes. The hetero-tetramer disrupted the acyl chain orders of both PC and PS in the PS-containing raft membrane, but only the GM1 in the GM1-containing raft membrane as effectively as the homo-tau-tetramer. We discovered that the alpha-helical content in the heterodimer was greater than the sum of alpha-helical contents from isolated tau and amylin monomers on both raft membranes, indicative of a synergetic effect of tau-amylin interactions in surface-induced protein folding. Our results provide new molecular insights into understanding the cross-talk between Alzheimer’s and diabetes.
Cross-Sectional Study of the Anthropometric Profile and Nutrient Status of Elite Female Ice Hockey Players: Differences by Play Position
Authors: Jiménez-Casquet María José , Conde-Pipo Javier , Valenzuela-Barranco Ignacio , Rienda-Contreras Raquel , Olea-Serrano Fátima , Monserrat-Mesquida Margalida , Tur Josep A. , Bouzas Cristina , Mariscal-Arcas Miguel
Published: February 2024

Both the characteristics of ice hockey and the environmental conditions in which it is played affect the maintenance of the player’s nutritional status and, therefore, their state of health and performance. The primary aim of this work was to examine the anthropometric profile, estimated energy expenditure, and macronutrient and micronutrient dietary intake of elite female ice hockey players by play position. As a secondary aim, their dietary intakes were compared with the recommendations. Hypotheses suggest variations in body composition based on ice hockey players’ positions, with the expectation that these athletes may not align with energy and nutrient recommendations. Fifteen elite female ice hockey players were anthropometrically measured, basal metabolic rate and total energy expenditure were estimated, a 3-day, 24 h recall questionnaire was registered, and the results were compared with the recommended dietary intake for the Spanish population. Each player’s position on the field requires an individualized physical and nutritional approach. There are no significant imbalances (p > 0.05) between energy and nutrient intake in relation to the Recommended Daily Intake (RDI); however, increasing the consumption of vegetables and seafood while reducing meat and fat intake would assist these athletes in maintaining a healthier energy balance, optimizing body composition, and aligning with nutritional parameters that are better suited to enhance physical performance.
Efficient Identification of Crude Oil via Combined Terahertz Time-Domain Spectroscopy and Machine Learning
Authors: Yang Fan , Ma Huifang , Huang Haiqing , Li Dehua
Published: February 2024

The quality of crude oil varies significantly according to its geographical origin. The efficient identification of the source region of crude oil is pivotal for petroleum trade and processing. However, current methods, such as mass spectrometry and fluorescence spectroscopy, suffer problems such as complex sample preparation and a long characterization time, which restrict their efficiency. In this work, by combining terahertz time-domain spectroscopy (THz-TDS) and a machine learning analysis of the spectra, an efficient workflow for the accurate and fast identification of crude oil was established. Based on THz-TDS of 83 crude oil samples obtained from six countries, a machine learning protocol involving the dimension reduction of spectra and classification was developed to identify the geological origins of crude oil, with an overall accuracy of 96.33%. This work demonstrates that THz spectra combined with a modern numerical scheme analysis can be readily employed to categorize crude oil products efficiently.
Smart and UV-Resistant Edible Coating and Films Based on Alginate, Whey Protein, and Curcumin
Authors: Botalo Atcharaporn , Inprasit Thitirat , Ummartyotin Sarute , Chainok Kittipong , Vatthanakul Suteera , Pisitsak Penwisa
Published: February 2024

In this work, smart edible coating and films with excellent UV barrier properties were prepared from alginate, whey protein isolate, and curcumin. The primary focus of this investigation centered on assessing the impact of whey protein and curcumin on the physical and functional properties of the alginate films. Whey protein reduced the film transparency while simultaneously enhancing the hydrophobicity and antioxidant properties of the alginate film. Curcumin imparted a yellow hue to the film, consequently decreasing the transparency of the film. It also substantially improved hydrophobicity, antioxidant activity, and UV-blocking efficiency within the films. Remarkably, curcumin demonstrated a significant reduction in the water vapor transmission rate of the film. For the preservation of apples, a higher concentration of curcumin was required, which effectively suppressed the respiration rate and moisture loss post-harvest, resulting in an extended shelf-life for the apples. As a result, the coated apples exhibited significantly reduced enzymatic browning and weight loss in comparison to their uncoated counterparts. Furthermore, these curcumin-containing films underwent a reversible color change from orange to red when exposed to ammonia vapor. This attribute highlights the potential of the developed coating and film as a smart, active food packaging solution, particularly for light-sensitive food products.
The Hangzhou Region and the Spread of East Asian Buddhism
Authors: Welter Albert
Published: February 2024

The story of East Asian Buddhism is a complex tapestry woven through centuries of cultural exchange, religious development, and scholarly interpretations [...]
A Novel Automatic Registration Method for Array InSAR Point Clouds in Urban Scenes
Authors: Cui Chenghao , Liu Yuling , Zhang Fubo , Shi Minan , Chen Longyong , Li Wenjie , Li Zhenhua
Published: February 2024

The array interferometric synthetic aperture radar (Array InSAR) system resolves shadow issues by employing two scans in opposite directions, facilitating the acquisition of a comprehensive three-dimensional representation of the observed scene. The point clouds obtained from the two scans need to be transformed into the same coordinate system using registration techniques to create a more comprehensive visual representation. However, the two-point clouds lack corresponding points and exhibit distinct geometric distortions, thereby preventing direct registration. This paper analyzes the error characteristics of array InSAR point clouds and proposes a robust registration method for array InSAR point clouds in urban scenes. It represents the 3D information of the point clouds using images, with pixel positions corresponding to the azimuth and ground range directions. Pixel intensity denotes the average height of points within the pixel. The KAZE algorithm and enhanced matching approach are used to obtain the homonymous points of two images, subsequently determining the transformation relationship between them. Experimental results with actual data demonstrate that, for architectural elements within urban scenes, the relative angular differences of registered facades are below 0.5°. As for ground elements, the Root Mean Square Error (RMSE) after registration is less than 1.5 m, thus validating the superiority of the proposed method.
Probabilistic Estimation of Tropical Cyclone Intensity Based on Multi-Source Satellite Remote Sensing Images
Authors: Song Tao , Yang Kunlin , Li Xin , Peng Shiqiu , Meng Fan
Published: February 2024

Estimating the intensity of tropical cyclones (TCs) is beneficial for preventing and reducing the impact of natural disasters. Most existing methods for estimating TC intensity utilize single-satellite or single-band remote sensing images, but they lack the ability to quantify the uncertainty of the estimation results. However, TC, as a typical chaotic system, often requires confidence intervals for intensity estimates in real-world emergency decision-making scenarios. Additionally, the use of multi-source image inputs contributes to the uncertainty of the model. Consequently, this study introduces a neural network (MTCIE) that utilizes multi-source satellite images to provide probabilistic estimates of TC intensity. The model utilizes infrared and microwave images from multiple satellites as inputs. It uses a dual-branch self-attention encoder to extract TC image features and provides uncertainty estimates for TC intensity. Furthermore, a dataset for estimating the intensity of multi-source TC remote sensing images (MTCID) is constructed through the registration of latitude, longitude, and time, along with data augmentation. The proposed method achieves a MAE of 7.42 kt in deterministic estimation, comparable to mainstream networks like TCIENet. In uncertain estimation, it outperforms methods like MC Dropout in the PICP metric, providing reliable probability estimates. This supports TC disaster emergency decision making, enhancing risk mitigation in real-world applications.
A Preliminary Investigation about the Influence of WIMU PROTM Location on Heart Rate Accuracy: A Comparative Study in Cycle Ergometer
Authors: Marzano-Felisatti Joaquín Martín , De Lucca Leonardo , Guzmán Luján José Francisco , Priego-Quesada Jose Ignacio , Pino-Ortega José
Published: February 2024

Technological development has boosted the use of multi-sensor devices to monitor athletes’ performance, but the location and connectivity between devices have been shown to affect data reliability. This preliminary study aimed to determine whether the placement of a multi-sensor device (WIMU PROTM) could affect the heart rate signal reception (GARMINTM chest strap) and, therefore, data accuracy. Thirty-two physical education students (20 men and 12 women) performed 20 min of exercise in a cycle ergometer based on the warm-up of the Function Threshold Power 20 test in laboratory conditions, carrying two WIMU PROTM devices (Back: inter-scapula; Bicycle: bicycle’s handlebar—20 cm from the chest) and two GARMINTM chest straps. A one-dimensional statistical parametric mapping test found full agreement between the two situations (inter-scapula vs. bicycle’s handlebar). Excellent intra-class correlation values were obtained during the warm-up (ICC = 0.99, [1.00–1.00], p < 0.001), the time trial test (ICC = 0.99, [1.00–1.00], p < 0.001) and the cool-down (ICC = 0.99, [1.00–1.00], p < 0.001). The Bland–Altman plots confirmed the total agreement with a bias value of 0.00 ± 0.1 bpm. The interscapular back placement of the WIMU PROTM device does not affect heart rate measurement accuracy with a GARMINTM chest strap during cycling exercise in laboratory conditions.
A Novel Approach to Evaluating Crosstalk for Near-Infrared Spectrometers
Authors: Chen Zemeng , Cao Xinliang , Li Xianglin , Pan Boan , Wang Pengbo , Li Ting
Published: February 2024

Multi-channel and multi-parameter near-infrared spectroscopy (NIRS) has gradually become a new research direction and hot spot due to its ability to provide real-time, continuous, comprehensive indicators of multiple parameters. However, multi-channel and multi-parameter detection may lead to crosstalk between signals. There is still a lack of benchmarks for the evaluation of the reliability, sensitivity, stability and response consistency of the NIRS instruments. In this study, a set of test methods (a human blood model test, ink drop test, multi-channel crosstalk test and multi-parameter crosstalk test) for analyzing crosstalk and verifying the reliability of NIRS was conducted to test experimental verification on a multi-channel (8-channel), multi-parameter (4-parameter) NIRS instrument independently developed by our team. Results show that these tests can be used to analyze the signal crosstalk and verify the reliability, sensitivity, stability and response consistency of the NIRS instrument. This study contributes to the establishment of benchmarks for the NIRS instrument crosstalk and reliability testing. These novel tests have the potential to become the benchmark for NIRS instrument reliability testing.
Monitoring SF6 Gas Leakage Based on a Customized Binocular System
Authors: Si Wenrong , Zhao Yingying , Wang Yan , Li Ben , Tong Geng , Yu Yiting
Published: February 2024

Sulfur hexafluoride (SF6) gas is extensively utilized as an insulating and arc-quenching medium in the circuit breakers and isolating switches of electrical equipment. It effectively isolates the circuits from the atmosphere and promptly extinguishes arcs. Therefore, the issue of SF6 gas leakage poses a significant threat to the related application fields, and the detection of SF6 gas leakage becomes extremely important. Infrared imaging detection offers advantages including non-contact, high precision, and visualization. However, most existing infrared detection systems are equipped with only one filter to detect SF6 gas. The images captured contain background noise and system noise, making these systems vulnerable to interference from such noises. To address these issues, we propose a method for monitoring SF6 gas leakage based on a customized binocular imaging (CBI) system. The CBI system has two filters, greatly reducing the interference of system noise and background noise. The first filter features the absorption resonant peak of SF6 gas. The second filter is used to record background noise and system noise. One aspect to note is that, in order to avoid the interference of other gases, the central wavelength of this second filter should keep away from the absorption resonant peaks of those gases. Accordingly, the central wavelengths of our customized filters were determined as 10,630 nm and 8370 nm, respectively. Then, two cameras of the same type were separately assembled with a customized filter, and the CBI prototype was accomplished. Finally, we utilized the difference method using two infrared images captured by the CBI system, to monitor the SF6 gas leakage. The results demonstrate that our developed system achieves a high accuracy of over 99.8% in detecting SF6 gas. Furthermore, the CBI system supports a plug-and-play customization to detect various gases for different scenarios.