bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-Reported Mental Health During the COVID-19 Pandemic and its Association with Alcohol and Cannabis Use: A Latent Class Analysis
Authors: Somé Nibene Habib , Wells Samantha , Felsky Daniel , Hamilton Hayley A. , Ali Shehzad , Elton-Marshall Tara , Rehm Jürgen
Published: September 2021

Background: Mental health problems and substance use co-morbidities during the COVID-19 pandemic are a public health priority. Identifying individuals at high-risk of developing these problems can directly inform mitigating strategies. We aimed to identify distinct groups of individuals (i.e., latent classes) based on patterns of self-reported mental health symptoms and investigate associations with alcohol and cannabis use.Methods: We used data from six successive waves of a web-based cross-sectional survey of adults aged 18 years and older living in Canada (6,021 participants). We applied latent class analysis to three domains of self-reported mental health: anxiety, depression, and loneliness. Logistic regression was used to characterize latent class membership, estimate the association of class membership with alcohol and cannabis use, and perform sex-based analyses.Results: We identified two distinct classes: 1) individuals with low scores on all three mental health indicators (no/low-symptoms) and 2) those reporting high scores (high-symptoms). Those at greater risk of being in the high-symptoms class were likely to be women (adjusted odds ratio (aOR) =1.34, 95%CI:1.18-1.52), people worried about getting COVID-19 (aOR=2.39, 95%CI:2.02-2.82), and those with post-secondary education (aOR=1.26, 95%CI:1.02-1.55). Asian ethnicity (aOR=0.78, 95%CI:0.62-0.97), married status (aOR=0.71, 95%CI:0.59-0.85), seniors (aOR=0.38, 95%CI:0.32-0.47), individuals in households with income higher than CAD$40,000: $40,000-$79,000 (aOR=0.73, 95%CI:0.60-0.90), $80,000-$119,000 (aOR=0.60, 95%CI:0.48-0.74) and $120,000+ (aOR=0.47, 95%CI:0.37-0.59) were at lower odds of being in the high-symptoms class. Individuals in the high-symptoms class were more likely to use cannabis at least once a week (aOR=2.25, 95%CI:1.90-2.67), drink alcohol heavily (aOR=1.69, 95%CI:1.47-1.95); and increase the use of cannabis (aOR=3.48, 95%CI:2.79-4.35) and alcohol (aOR=2.37, 95%CI:2.05-2.73) during the pandemic. Women in the high-symptoms class had higher odds of increasing alcohol use than men.Conclusions: We identified the determinants of experiencing high-symptoms of anxiety, depression, and loneliness, and found a significant association with alcohol and cannabis consumption. This suggests that initiatives and supports are needed to address mental health and substance use multi-morbidities, particularly regarding alcohol use in women.
Polycyclic Aromatic Hydrocarbons (PAHs) In The Upstream Rivers of Taihu Lake Basin, China: Spatial Distribution, Sources And Environmental Risk
Authors: Wang Lingling , Ren Xiaoming , Wang Xiaoping , Ye Perran , Wang Fei , Cheng Jiade , Chen Yan , Yu Ang , Zhang Limin , Qiu Yang
Published: September 2021

The polycyclic aromatic hydrocarbons (PAHs) pollution in Taihu Lake Basin has caused widespread concern. However, the spatial temporal distribution of PAHs in the upstream rivers of Taihu Lake Basin remains largely unknown. Thus, this study aims to investigate the level, spatial distribution, sources and environment risk caused by PAHs in upstream rivers of Taihu Lake Basin. The concentrations of total 16 PAHs (∑16PAHs) ranged from 188.64 to 1060.39 ng/g, with an average of 472.62 ng/g. Compared with low-molecular-weight (LMW) PAHs, high-molecular-weight (HMW) PAHs were more resistant to degradation and easier to accumulate in the sediment. The results of source analysis demonstrated that the PAH pollution was mainly sourced from mixture of fuel combustion and direct petroleum spillage. The ecological risk assessment showed that moderate ecological risk caused by the PAH contaminants might occur in most sample sites. The incremental lifetime cancer risk (ILCRs) ranged from 2.07 ×10-4 - 2.66 × 10-3 for children and 9.66 ×10-5 - 1.24 × 10-3 for adult, indicating moderate cancer risk of PAH-contaminated sediments.
Spectrum of Heart Diseases at a Referral Tertiary Care Hospital in Somalia, Mogadishu: An Echocardiographic Study
Authors: Alıcı Gökhan , Genç Ömer
Published: September 2021

Background: To investigate the frequencies and patterns of cardiovascular diseases (CVD), including rheumatic and congenital heart diseases, assessed by echocardiographic examinations in the only referral tertiary care hospital in Mogadishu, the capital of Somalia.Methods: This retrospective, descriptive registry reviewed the pathological echocardiographic findings of 1140 patients aged 0-100 years who were admitted to the cardiology outpatient clinic in a tertiary training hospital in Mogadishu.Results: Hypertensive heart disease (HHD) 454 (39.8%) and degenerative valvular disease 395 (34.6%) were the most common comorbidities. Congenital heart diseases (CHD) were detected in 151 (13.2%) of the patients, with the most common ones including atrial septal defect (ASD) 37 (3.2%) and ventricular septal defect (VSD) 26 (2.3%).Rheumatic heart disease (RHD) was detected in 84 (7.4%) patients, among whom the most common age range was 16-30 years (40.5%), followed by 31-45 years (31%) and 0-15 years (15.5%). Conclusıon: In the present study, we found that HHD was the most common comorbidity , followed by degenerative valvular disease, Heart failure with reduced ejection fraction(HFrEF),and Ischemic heart disease(IHD).Moreover, the most common valvular disease was mitral insufficiency and the most common CHD was ASD.
Tinospora Cordifolia Leaves Derived Carbon Dots For Cancer Cell Bioimaging, Free Radical Scavenging, And Fe3+ Sensing Applications
Authors: Mohapatra Debadatta , Pratap Ravi , Pandey Vivek , Dubey Pawan K. , Agrawal Ashish K. , Parmar Avanish S. , Sahu Alakh Niranjan
Published: September 2021

Herein, we report the fabrication of Tinospora cordifolia leaves-derived carbon dots (TCLCDs) from aqueous extract of leaves as carbon source via simple, environmental friendly, hydrothermal carbonization (HTC) technique. The synthesized TCLCDs were characterized for their physicochemical properties and further explored for in-vitro cancer cell bioimaging, radical scavenging, and metal ion sensing. The synthesized TCLCDs showed excitation-dependent emission property with maximum emission at 435 nm under the excitation of 350 nm. The High-Resolution Transmission Electron Microscopy (HRTEM) results revealed a roughly spherical shape with an average diameter of 5.47 nm. The diffused ring pattern of Selected Area Electron Diffraction (SAED) and halo diffraction pattern of X-ray diffraction (XRD) disclosed their amorphous nature. The Energy Dispersive X-ray (EDX) showed the existence of C, N, and O. The Fourier-transform infrared spectroscopy (FTIR) revealed the presence of -OH, -NH, -CN, and -CH groups. The TCLCDs showed excellent cellular biocompatibility with dose-dependent bioimaging results in melanoma (B16F10) and cervical cancer (SiHa) cell lines. Also, they exhibited excellent scavenging of free radicals with an IC50 value of 0.524 mg/mL & selective Fe3+ ion sensing with a detection limit of 0.414 µM. Further, they exerted excellent bacterial biocompatibility, photostability, and thermal stability. The overall results reflected their potential for in-vitro cancer cell bioimaging, free radical scavenging, and selective Fe3+ ion sensing.
Enhanced Performance of Natural Polymer Coagulants For Dye Removal From Wastewater: Coagulation Kinetics, And Mathematical Modelling Approach
Authors: Obiora-Okafo Ifeoma Amaoge , Onukwuli Okechukwu Dominic , Igwegbe Chinenye Adaobi , Onu Chijioke Elijah , Omotioma Monday
Published: September 2021

This study explores the potentials of Brachystegia eurycoma coagulant (BEC) and Vigna subterranean coagulant (VSC) as natural organic polymers (NOPs) for the decolourisation of Crystal Ponceau 6R (AR 44) in wastewater. Materials characterisation studies were done on the precursors. Detailed kinetics study was employed. The decolourisation procedures were evalu­ated through time-dependent reduction in the concentration of particles, with the variation of the independent parameters. The proximate analysis showed protein contents of 19.77% and 18.15% for BEC and VSC, respectively. The functional test shows the presence of –OH, N–H, and C=H. Surface morphological study reveals that some rough surfaces, different pores sizes, and compact-net structures were evidenced. The order of removal efficiency was VSC > BEC with an optimum of 88.8% and 73.3%, respectively. The values of K and α obtained for BEC and VSC were 6.38 x 10-4 Lmg-1min-1, 1.8 and 4.03 x 10-3 L/mg-1min-1, 1.9, respectively. The coagulation time, Tag of 31.35 and 26.96 min for BEC and VSC respectively disclosed a quick coagulation. The coagulation-flocculation kinetics demonstrated that the process conforms to the pseudo-second-order model with R2 >0.997, suggesting that the rate-controlling mechanism is governed by chemisorption. The experimental data were well predicted by the cross-validation test, with percentage mean relative deviation modulus (M%) of 3.26 and 2.54 for BEC and VSC, respectively. These coagulants have added meaningful progress in wastewater treatment by coagulation-flocculation while displaying significant adsorption features. Likewise, the usage of kinetics studies and particle behaviour modelling should be a prerequisite in water treatment processes.
A New Fluoride Chemodosimeter Based On 3-Ether-Substituted 1, 8-Naphthalimide Derivatives
Authors: Chen Xi , Shao Haibing , Zhu Tingting , Chen Zhihua , Hu Yan , Zhang Hua , Liu Chuanxiang
Published: September 2021

We developed a new chromogenic and fluorescent "off–on" 1,8-naphthalimide-derivated chemosensor 1 based on an F−-triggered desilylation reaction. It showed significant variations in UV/Visible absorption (510 nm) and fluorescence emission wavelength (570 nm) for selective detection of fluorides in tetrahydrofuran. Moreover, chemodosimeter 1-loaded test strips were successfully fabricated todetect fluorides efficiently.
Assessment of The Effect of Interprofessional Professionalism Training On The Surgical Team Members' Perception of The Ethical Climate
Authors: Keshmiri Fatemeh , Hoseinpour Azam
Published: September 2021

BackgroundThe aim of present study was to assess the effect of interprofessional education concerning interprofessional professionalism on learners' perception of the ethical climate of the operating room.MethodThe present study is quasi-experimental design. Learners include surgical residents, operating room technicians, and anesthesia technicians (n=130) that distributed to intervention and control groups. The objectives of the intervention were the development of competencies of ethics and professionalism (including communication, altruism, respect, and excellence). The educational strategy was interprofessional education and the main method of training was scenario-based learning. Participants completed the Olson Moral Climate Questionnaire before and one month after the intervention. We used descriptive tests (mean, SD, percentage), student t-test and ANOVA to compare the scores of learners in the intervention and control groups.ResultsThe results of the study showed that the scores of the learners in the intervention group 4.05 (0.31) improved significantly compared to the control group 3.35 (0.37) (P = 0.0001). The scores of learners in the domain of ​​“managers” improved higher and the domain of ​​physicians improved lesser than other domains. There was no significant difference between the three groups of residents, operating room, and anesthesia technicians in terms of moral climate scores.ConclusionIn the present study showed the positive effect of interprofessional education interventions on individuals' perceptions of the operating room ethical climate. It is suggest that educational interventions be planned and implemented continuously in the educational systems and hospitals.
Improved Fertiliser Management To Reduce The Greenhouse-Gas Emissions And Ensure Yields In A Wheat–Peanut Rotation System In China
Authors: Liu Zhaoxin , Zhao Chen , Zhao Jihao , Lai Huajiang , Li Xiangdong
Published: September 2021

Over the last century, anthropogenic greenhouse-gas (GHG) emissions have changed the global climate, and agriculture plays an important role in the global flux of GHG. Agricultural management practices, such as split N applications and the use of controlled-release fertilisers have significantly increased the crop yield and N-use efficiency by balancing the N demand of crops and the N availability of soils. However, the impacts of these practices on GHG emissions (in particular in wheat–peanut relay intercropping systems) have not been evaluated in detail. In this study, we used a common compound fertiliser and a controlled-release compound fertiliser (CRF) applied the day prior to sowing, at the jointing stage of wheat, and at the anthesis stage of peanut in various proportions, with a control treatment of 0 kg ha-1. The findings demonstrated that treatment JCF70 achieved increases in yields of 9.7% and 14.6% for wheat grain and peanut pod, respectively, compared to treatment JCF100; however, this treatment also significantly increased soil emissions of CO2 and N2O. In addition, cumulative emissions of CO2 and N2O were higher in the peanut growing season by 74.4 and 31.7%, respectively, than in the wheat growing season owing to the relatively higher soil temperature during the former season. Fertilisation combined with irrigation, was found to be the main cause of GHG emissions. Under the same fertiliser rate and N-management style, JCRF70 further increased the yield of peanut pods and the total combined yield of peanut and wheat by 10.3% and 8.9%, respectively, compared to treatment JCF70. The cumulative CO2 and N2O emissions in treatment JCRF70 were 20.4–45.4% less than those in treatment JCF70. The total global warming potentials of CO2 and N2O were lowest in treatment JCRF70 owing to it providing the highest grain yield. Therefore, N application with three splits, together with the use of a slow-release fertiliser, may be a simple and effective approach to enhance the grain yield while reducing the GHG emissions.
Tissue fluidification promotes a cGAS/STING-mediated cytosolic DNA response in invasive breast cancer
Authors: Frittoli Emanuela , Palamidessi Andrea , Iannelli Fabio , Zanardi Federica , Villa Stefano , Barzaghi Leonardo , Ando Hind , Cancila Valeria , Beznuskenko Galiba , Chiara Giulia Della , Pagani Massimiliano , Malinverno Chiara , Bhattacharya Dipanjan , Pisati Federica , Yu Weimiao , Galimberti Viviana , Bonizzi Giuseppina , Martini Emanuele , Mironov Alexander , gioia ubaldo , fagagna Fabrizio D'adda di , Rossi Chiara , Bertalot Giovanni , Lucioni Marco , tancredi richard , Pedrazzoli paolo , Vecchione andrea , Perini Cristiuano , Ferrari FRancesco , Lanzuolo Chiara , Nader Guilherme , Foiani Marco , Piel Matthieu , Cerbino Roberto , Giavazzi Fabio , Tripodo Claudio , Scita Giorgio
Published: September 2021

The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from a solid-like to a liquid-like state that occurs in various tissues. Whether this tissue-level mechanical transition impact phenotypes during carcinoma progression remains unclear. We show, here that the large fluctuations in cell density that accompany unjamming result in repeated mechanical deformations of cells and nuclei. Cells react to these protracted mechanical stresses by mounting a mechano-protective response that includes enlarged nuclear size and rigidity, altered heterochromatin distribution, and the remodeling of the perinuclear actin architecture into actin rings. The chronic strains and stresses associated with unjamming together with reduction of Lamin B1 levels eventually result in DNA damage and nuclear envelope ruptures, with the release of cytosolic DNA that activates a cGAS/STING-dependent cytosolic DNA response gene program. This mechanically-driven transcriptional rewiring ultimately results in a change in cell state, with the emergence of malignant traits, including epithelial-to-mesenchymal plasticity phenotypes and chemo-resistance in invasive breast carcinoma.

One-Sentence Summary: A solid-to-fluid phase transition promotes a pro-inflammatory transcriptional response in invasive breast carcinoma
Range Extension and Species Confirmation of Rhyneptesicus Nasutus (Sind Serotine Bat) (Mammalia: Chiroptera) From Bajaur Agency, FATA, Pakistan
Authors: Idnan Muhammad , Mansoor Sajid , Khawar Muhammad Babar , Javid Arshad , Hussain Ali , Imran Muhammad , Ullah Arif
Published: September 2021

The lack of morphological differentiation among chiropteran species and cryptic speciation impedes species identification. So, DNA-based approaches boost species identification and are increasing biological diversity. Rhyneptesicus nasutus (Sind Serotine Bat) is a rarely distributed and poorly studied species in Pakistan. This study explores the range extension of Sind bat within the territorial limits of Pakistan from Sind and Baluchistan to Federally Administered Areas (FATA) of Pakistan. No molecular record exists for the species in Pakistan. In the present study, we for the first time present the data to confirm the species identification by genetic marker (cytochrome b) along with morphometric analysis of Rhyneptesicus nasutus from Pakistan. The neighbor-joining tree based on Kimura-2 parameters was created to infer the phylogenetic analyses. We sequenced the cytochrome b gene segment and conducted a phylogenetic analysis with previously published data from other countries. Sequences from Pakistan made a clade with Iranian Rhyneptesicus nasutus species suggesting a common ancestry. Various morphometric parameters (mean values) were measured like Head and Body length (44.3mm), Tail length (43.4mm), Hindfoot length (8.3mm), Forearm length (35.7mm), and Ear length 36mm while 5th Metacarpal Length, 4th Metacarpal Length, and 3rd Metacarpal Lengths were 33.2mm, 34.7mm, 35.3mm. Approaches based on DNA barcoding reveals high diversity of bat species in the study area. The data will enable researchers to build an improved evolutionary landscape of the Serotine bats from this region and subsequently reconstruct a detailed evolutionary history of the genus. Further research is required to test other molecular markers to support the findings of the current study in Pakistan.
Effect of Physical Properties of Synthesized Protic Ionic Liquid On Carbon Dioxide Absorption Rate
Authors: Chaudhary Amita , Bhaskarwar Ashok N
Published: September 2021

Concentration of carbon dioxide gas has accelerated from the last two decades which cause drastic changes in the climatic conditions. In industries, carbon capture plants use volatile organic solvent which causes many environmental threats. So, a low-cost green absorbent has been formulated with nontoxicity and high selectivity properties for absorbing carbon dioxide gas. This paper contains the synthesis process along with the structure confirmation using 1H NMR, 13C NMR, FT-IR, and mass spectroscopy. Density, viscosity, and diffusivity are measured at different ranges with standard instruments. The kinetic studies were also conducted in a standard predefined-interface stirred-cell reactor. The kinetic parameters were calculated at different parameters like agitation speeds, absorption temperature, initial concentrations of ionic liquid, and partial pressure of carbon dioxide. The reaction regime of carbon dioxide absorption is found to be in fast reaction kinetics with pseudo first order. The reaction rate and the activation energy of CO2 absorption are experimentally determined in the range of 299 K to 333K with different initial concentrations of ionic liquid (0.1-1.1 kmol/m3). The second order rate constant and activation energy of carbon dioxide absorption in the synthesized ionic liquid is found to be (6385.93 to 12632.01 m3 mol-1 s-1) and 16.61 kJ mol−1 respectively. This solvent has shown great potential to absorb CO2 at large scale.
SETD2 Deficiency and miR-21: Potent Therapeutic Targets in NUT Midline Carcinoma
Authors: YOSHIDA NANA , OKUMURA SHUNSUKE , SASAKI TAKAAKI , CHIBA SHIN-ICHI , SADO MASATOSHI , OYAMA KYOHEI , YOSHIDA RYOHEI , HIRAI NORIKO , MINAMI YOSHINORI , KITADA MASAHIRO , OHSAKI YOSHINOBU
Published: September 2021

BackgroundNuclear protein in testis (NUT) midline carcinoma (NMC) is a rare and highly aggressive tumor with the bromodomain containing 4 protein (BRD4)-NUT (NUTM1) gene fusion. Few targeted therapies are available for NMC, and novel therapeutic targets are required. The objectives of our study was to determine gene mutations and microRNA (miRNA) expression levels in NMC and to identify novel therapeutic targets for NMC. Methods Next-generation sequencing (NGS) was used to identify mutations in the NMC cell lines and the tumor sample from a NMC patient; we determined the BRD4-NUT fusion gene in the patient using a digital PCR assay. Trimethylation of lysine 36 on histone H3 expression (H3K36me3) was studied using western blotting. The efficacy of the WEE1 G2 checkpoint kinase (WEE1) inhibitor, AZD1775, was evaluated using the MTS assay. RNA sequencing was performed to determine miRNA expression levels in the NMC cell lines. TaqMan miRNA assays were used to analyze miR-21 expression. The efficacy of miR-21 inhibitor was evaluated using the MTS assay. Results A novel SETD2 mutation (p.Ser2382fs) was identified in the NMC cell lines and the patient tumor. H3K36me3 was depleted in the NMC cells, which was indicative of functional SETD2 loss. The NMC cells were sensitive to AZD1775, an inhibitor for SETD2-deficient cancer. miRNA analysis revealed that miR-21 expression was increased in the NMC cells resistant to the traditional BET-targeted therapy. The miR-21 inhibitor suppressed the proliferation of the NMC cells. ConclusionsSETD2 deficiency and miR-21 may serve as novel therapeutic targets for the treatment of NMC.Trial registrationIn this study, we analyzed the gene alterations in human tumor samples. This study was registered in the UMIN clinical trial registered system (UMIN000043147, January 27, 2021).
Prevalence of Hypothyroidism Among Patients With COVID-19, Tehran, Iran
Authors: Esfahanian Fatemeh , SeyedAlinaghi SeyedAhmad , Janfaza Nazanin , Tantuoyir Marcarious M.
Published: September 2021

Objective: Numerous comorbidities are involved in the severity of Corona Virus Disease 2019 (COVID-19). Hypothyroidism's impact on COVID-19 is yet to be understood properly. Cellular immunity and different cytokines, on the other hand, are thought to play a role in the development and progress of COVID-19 and thyroid disease, according to earlier research. The goal of this study was to find out how common (prevalent) hypothyroidism was among COVID-19 patients and its possible influence on the disease prognosis on hospitalized patients at a Tehran University Hospital.Methods: Demographic information and other data related to our study, mainly comorbidities, were collected from 493 COVID-19 patients’ medical records and analyzed.Results: We identified that hypothyroidism was significantly prevalent in hospitalized COVID-19 patients than in the general population. Overall, in patients with hypothyroidism (n=65), n=21(32.3%) patients expired of whom 14 (66.7%) patients were females and 7 (33.3%) were males. In these patients, no statistically significant difference was observed between the expired and discharged groups (Adjusted Odds Ratio (ORadj): 1.04 (95% Confidence Interval (CI): 0.59-1.83 p-value=0.87). As a result, hypothyroidism was not linked to an increased risk of death.Conclusion: In this current study, we demonstrated that the prevalence of hypothyroidism was higher in hospitalized COVID-19 patients than in the normal population especially in females but without significant adverse effects on the risk of mortality from this disease.
Long Noncoding RNA KCNQ1OT1 Induces Resistance of HCC Cells To Cisplatin Through Regulating The miR-26a/CCND2 Molecular Axis
Authors: LI Cai , YE Qi-Fa
Published: September 2021

Objective: To explore the molecular mechanism by which LncRNA KCNQ1OT1 regulated the miR-26a/CCND2 molecular axis to participate in the resistance of Hepatocellular carcinoma(HCC) cells to cisplatin.Methods: Cancer tissue and corresponding para-carcinoma tissue specimens were collected from 25 HCC patients with complete data admitted from January 2018 to December 2018 at The Transplantation Center of the Third Xiangya Hospital. Then, the expression levels of KCNQ1OT1, miR-26a and CCND2 in HCCtissues and cell lines were detected through qRT-PCR. Meanwhile, the sensitivity of HCC cells to cisplatin was examined through Transwell and Annexin V-FITC/PI double staining flow cytometry. Further, the targeted relationships among KCNQ1OT1, miR-26a and CCND were verified through dual-luciferase reporter gene assay, and the regulatory relationships were detected through Western blotting and qRT-PCR.Results: KCNQ1OT1 was highly expressed in HCC tissues and cisplatin-resistant cell lines; meanwhile, over-expression of KCNQ1OT1 promoted the resistance of Huh7/CDDP cells to cisplatin. Dual-luciferase reporter gene assay verified that, KCNQ1OT1 targeted miR-26a and down-regulated its expression level. miR-26a suppressed Huh7/CDDP cell proliferation and invasion, while promoting their apoptosis, thus down-regulating the promoting effect of KCNQ1OT1 on the cisplatin resistance of HCC cells. miR-26a negatively regulated CCND2 expression, while KCNQ1OT1 down-regulated the suppression of miR-26a on CCND2 to promote Huh7/CDDP cell proliferation and invasion and to suppress apoptosis, thereby up-regulating the resistance of HCCcells to cisplatin. Conclusions: LncRNA KCNQ1OT1 regulates the miR-26a/CCND2 molecular axis to induce the resistance of HCC cells to cisplatin.
Strengthening the Service Experiences of Women Impacted by Gambling-related Intimate Partner Violence.
Authors: O'Mullan Cathy , Hing Nerilee , Nuske Elaine , Breen Helen , Mainey Lydia
Published: September 2021

BackgroundWhile problem gambling does not directly cause intimate partner violence (IPV), it exacerbates that violence significantly. Women experiencing both gambling harm and IPV often find themselves in challenging situations; furthermore, stigma and shame frequently act as barriers to seeking help from health and social service agencies. Despite the links between problem gambling and IPV, little is known about women’s experiences of using support services for both IPV and gambling related issues. This paper explores positive experiences of help-seeking for gambling-related IPV in Australia by adopting a strengths-based research approach. MethodsQualitative, unstructured interviews were conducted for a larger study exploring the nature of the relationship between problem gambling and IPV. To gain new insights into the service experiences of women impacted by gambling related IPV, interviews with 48 women with lived experience of IPV relating to a male partner's gambling, and 24 women with lived experience of IPV relating to their own gambling were reanalysed using thematic analysis.ResultsThree themes emerged from the data signifying or demonstrating strength-based responses: 'Commitment to Integrated and Collaborative Responses'; 'Therapeutic Support'; and 'Instrumental Support'. The themes highlight the importance of recognising the intersectionality of gambling related IPV and supporting the person 'at the centre of the service'. Tangible and instrumental supports, such as emergency accommodation and financial assistance, were also central to the recovery process. ConclusionEffective service responses are dependent on understanding how problem gambling and IPV intersect. Importantly, service providers must recognise and address the many facets of each woman's situation and the shame associated with resolving interdependent and complex issues. Responding to the needs of women impacted by gambling related IPV requires both individual-level awareness and organisational support; recommendations to strengthen service provision are provided.
Preoperative Venous Thromboembolism in Orthopedic Oncology Patients: Incidence, Risk Factors, and D-Dimer Cutoff Value
Authors: Hayashida Kenta , Kawabata Yusuke , Saito Keiju , Fujita Shintaro , Choe Hyonmin , Takeyama Masanobu , Inaba Yutaka
Published: September 2021

Background: Venous thromboembolism (VTE) is known as one of the major complications in patients with malignant tumors and orthopedic disorders. ﻿Patients undergoing surgery for orthopedic oncology are at an increased risk for thromboembolic events. However, there are limited studies regarding the incidence and risk factors for preoperative VTE in orthopedic oncology patients.Methods: The aim is to determine the incidence and risk factors of preoperative VTE by screening with D-dimer. We retrospectively reviewed the medical records of 270 patients who underwent surgical procedures, including biopsy for orthopedic oncology, had preoperative D-dimer levels measured and were subsequently screened for VTE by lower extremity venous ultrasonography and contrast-enhanced computed tomography (CT) scans if necessary. Statistical analyses were performed to examine the incidence and risk factors for VTE. Receiver operating characteristic (ROC) analysis was performed to verify the cutoff value of D-dimer for the diagnosis of VTE.Results: A total of 199 patients (103 with primary soft tissue sarcomas, 38 with primary bone sarcomas, 46 with metastatic tumors and 12 with hematologic malignancies) were included. The value of D-dimer was high in 79 patients. VTE was detected in 19 patients (9.5%). Multivariate analysis indicated that, age ≥60 years (P = 0.021), and tumor location in the lower limbs (P = 0.048) were independent risk factors for VTE. ROC analysis showed that the cutoff value of D-dimer for the diagnosis of VTE was 1.53 µg/mL (sensitivity of 89.5% and specificity of 79.4%).Conclusions: Our study indicated that age and tumor location in the lower limbs were independent risk factors for preoperative VTE in orthopedic oncology patients. D-dimer was not significantly different in the multivariate analysis because D-dimer is affected by a wide variety of conditions, such as malignancy and aging. Patients with high D-dimer levels and the abovementioned risk factors are at increased risk of preoperative VTE, and additional ultrasonography should be considered.Trial registration: Our study was approved by the institutional review board. The registration number is B200600056. The registration date was July 13, 2020.
The Association of Plasma Levels of miR-146a, miR-27a, miR-34a and miR-149 with Coronary Artery Disease
Authors: Mirzaei Ali , Hosseini Ebrahim syud , Hosseinpour sara , Khalvati Bahman , ainabad Farshad safari
Published: September 2021

Background: Coronary artery disease (CAD) is considered to be one of the most pivotal causes of death in the world. Over the past two decades, significant changes occurred in diagnosis, prognosis, and treatment of CAD, which has helped reduce mortality rates. miRNAs are a class of more than 5000 non-encoding RNA molecules (21 to 25 nucleotides across the length) that regulate the complex biological processes. Today, miRNAs are used to study cardiovascular diseases. In the present study, the expression of miR-146a،miR-27a ،miR-149 and miR-34a in plasma suffering from CAD and control group were investigated. Methods and Results: The present research was performed on 30 men with coronary artery stenosis (CAD) and 30 healthy men as controls. The expression levels of miR-146a, miR-27a, miR-149 and miR-34a in the plasma of patients with CAD and control group were measured using real-time PCR. Also, the correlation between the expression of circulating miRNAs levels and biochemical LDL-C, HDL-C, BMI, and cholesterol were evaluated. The expression of miR-27a in plasma of CAD group was higher than control group (p=0.020). The expression of miR-146a was downregulated in CAD patients compared to of non-CAD group (p=0. 026). However, the expression of miR-34a, miR-149 in the plasma of CAD patients were not significantly difference with the control group. In addition to, a direct correlation was found between the expression of miR-146a and HDL-c, the expression of miR-27a and LDL-C and the expression of miR-34a and total cholesterol. Also, the negative correlation between expressions of miR-149 with BMI was reported. Conclusion. The obtained results demonstrated that miRNAs was closely related to biochemical factors and it points out the fact that miRNAs can be applied as a potential strategy for diagnosis and treatment of CAD.
Short Term Effects of Mild Severity COVID-19 on Left Ventricular Functions
Authors: Bektaş Osman , Karagöz Ahmet , Yenerçağ Feyza Nur Topçu , Dereli Seçkin , Kurt Celali , Kaya Yasemin
Published: September 2021

Purpose: We aimed to evaluate long term effects of COVID-19 disease on left ventricular function using speckle tracking echocardiography. Methods: In our study, 96 non-hospitalized patients who were diagnosed with COVID-19 disease in our center between 15 August 2020 and 01 September 2020 and 96 age and gender matched control subjects were included.Results:The mean follow up duration was 137 ± 7 days. White blood cell, neutrophile and lymphocyte were significantly lower and C-reactive protein (CRP) and neutrophil to lymphocyte ratio (NLR) were significantly higher in the COVID-19 group. (6.6 ± 2.8 vs. 24.4 ± 21.8, p <0.001 and 4.1 ± 2.5 vs. 7.1 ± 4, p <0.001, respectively). LV-GLS and LV-GCS were significantly lower in the COVID-19 group. (-20.1 ± 3.1 vs -15.9 ± 2, p <0.001 and -29.3 ± 2.1 vs -26.3 ± 2.1, p <0.001, respectively). LV-GLS and LV-GCS were found to be negatively correlated with CRP (rs = -0.515, p <0.001 and rs = 0.-466, p <0.001, respectively) and NLR (rs = -0.494, p <0.001 and rs = -434, p <0.001, respectively). Conclusion: Deteriorating effects of COVID-19 disease on both LV-GCS and LV-GLS can be seen even in the short term. These detrimental effects seem to be associated with CRP levels and NLR measured in the course of active infection.
Determination of Prostate-Specific Antigen Via The Assembly of a Two-Dimensional Nanoplatform
Authors: Chen Junjie , Li Xiangqian , Yu Xiaoqi , Wang Qianming
Published: September 2021

Two-dimensional platforms with favorable features are highly expected for diverse application. In this work, we report a highly sensitive and selective “turn-on” fluorescent nanoprobe for prostate-specific antigen (PSA) detection base on cobalt oxyhydroxide nanosheets (CoOOH NSs). CoOOH NSs are employed as the suitable sensing hosts, in which fluorescein amidite-(abbreviated as FAM) labeled aptamer probe (PA) has been adsorbed on nanosheets. Energy transfer between substrate and optical species has switched off the fluorescence of PA. The strong affinity of PA to the target PSA induces the formation of a rigid aptamer structure and the integration with the CoOOH NSs has been drastically affected. The recognition process has been followed by the release of the aptamer probe PA from the nanosheet surface and the green luminescence has been recovered. The dynamic nano-sensor exhibits highly sensitive and accurate analytical performance toward PSA with a linear detection range from 0.1 to 5 nM and a detection limit of 56.1 pM. Therefore, a simple and efficient sensing platform for the detection of prostate cancer can be established.
Development, Validation, and Implementation of a Short Breast Health Questionnaire
Authors: Alipour Sadaf , Rashidi Hadi , Maajani Khadije , Orouji Marzieh , Eskandari Yas
Published: September 2021

Background

Health status and perception can be assessed by general or disease-specific questionnaires, the latter are more sensitive. Considering the importance of breast health in women’s lives and the lack of any pertinent questionnaire, we performed this study to develop a valid and reliable short BH questionnaire (BHQ); and then use it for the assessment of participants.
Methods

We first designed and developed the instrument, followed by measurement of inter-rater agreement IRA, content validity including content validity index (I-CVI) and scale content validity index (S-CVI), and reliability (through internal consistency and test-retest). We then included eligible women with normal breasts and benign breast disorders who attended our breast clinic.
Results

The IRA index (78.6%) showed optimal relevance and clarity of the questionnaire. The content validity was acceptable; with an S-CVI of 87.35 and 84.42 for clarity and relevance, respectively. One question was eliminated (I-CVI < 70%). The internal reliability was high (Cronbach’s alpha = 0.93). For external consistency, three questions were eliminated (intraclass correlation coefficient < 0.7), the rest of the questions showed good and excellent reliability. In the next step, BH in the 350 eligible participants showed an overall score of 55.86 ± 11.57. Among different variables, age was the only one that showed a significant direct relationship with BH. A history of breast surgery for benign lesions and a family history of breast cancer did not affect BH.
Conclusion

This study introduces a valid reliable 11-item BHQ. We propose its use in various conditions throughout breast cancer screening, diagnosis, and treatment; and in the assessment of BH in various physiologic and reproductive situations.
The Concurrence of Sexual Violence and Physical Fight Among Adolescent Suicide Ideators and the Risk of Suicide Attempt
Authors: Li Xun , Xiang Shi-Ting , Dong Jie
Published: September 2021

Experiences of sexual violence and physical fight had been identified as independent risk factors for suicidal behaviors among adolescents. The question raises whether the concurrence of these two risk factors further increases the risk of suicidal behaviors among ideators, and by how much the risk increases. This study analyzed data from the 2019 National Youth Risk Behavior Surveys. Students that reported had seriously considered attempting suicide in the past year were included (n=1755). The associations between physical fight/sexual violence status and the risk of suicide attempt/plan/injurious attempt were estimated. The concurrence of physical fight and sexual violence substantially increased the relative risks for attempted suicide (relative risk (RR)=2.01, 95%CI: 1.74, 2.31) and injurious suicide attempt (RR=4.03, 95%CI: 2.81, 5.78; subgroup analyses: among female, RR=3.25, 95%CI: 2.09, 5.04; among male, RR=6.19, 95%CI: 3.3, 12.14). Among students with concurrent physical fight and sexual violence, males reported more risk behaviors (median=14) than females (median=12)(P=0.0023). The concurrence of physical fight and sexual violence substantially increased the risks for attempted suicide among adolescent suicide ideators. A wide variety of risk behaviors clustered with the concurrence of physical fight and sexual violence, especially among males, which possibly lead to the higher rate of injurious suicide attempt among male students.
Auto-gyro Descent System for Earth’s Atmosphere
Authors: Nudurupati Abhay
Published: September 2021

An atmospheric descent mechanism that utilizes no power would be an efficient way to perform a safe landing procedure for small sized payloads and selected large payloads. This research paper presents the safe landing concept of an auto gyro descent system in the atmosphere of Earth, from a height of less than 1 Kilometre taken from the surface of executing point, using a specialized lift producing blade even at very low free stream velocity. These blades are designed to be highly cambered with suitable parameters as described further in the paper. Most of the parts are designed using CAD software and are 3D printed after a thorough simulation and analysis tests done using COMSOL Multi-physics and Xflr 5 for respective solutions. The model also includes the aerodynamics of the entire structure along with a base casing of specified structural design for further inclusion of electronic components for suitable scientific experiments. This provides a future base line for any kind of atmospheric studies or tests at a reasonable price and can be reused.
Effect of Deposition Time On Optical Properties of Chemical Bath Deposited Mercury Sulfide Thin Layers
Authors: Kangarlou Haleh , Asgary Somayeh
Published: September 2021

Mercury sulfide films were deposited on amorphous glass substrates from aqueous solutions by chemical bath deposition method (CBD) at same temperature and different deposition times. Produced layers were post annealed at 250°C about one hour. X-ray diffraction (XRD) was used to study of film’s crystalline structural. Their optical properties were measured by spectrophotometry in the spectra range of 400-850 nm, Kramers-Kronig method was used for the analysis of reflectivity curves of HgS films to obtain the optical constants of films in order to investigation of relation between deposition time and optical properties. According to X-ray diffraction details, all thin films showed crystalline phase with a preferential growth along the (220) planes. Optical results have been shown photolminisance property for HgS produced thin films. By increasing deposition time, the dielectric property, refractive index and band gap values are increased.
Perphenazine And Prochlorperazine Decrease Glioblastoma Cells Migration And Invasion: Analysis of ABCB1 And ABCG2 Transporters, e-cadherin, α-tubulin, And Integrins (α3, α5, and β1) Expression in U87-MG Cells
Authors: Otręba Michał , Stojko Jerzy , Kabała-Dzik Agata , Rzepecka-Stojko Anna
Published: September 2021

Purpose: Glioblastoma multiforme is the most frequent malignant brain tumor as well as one of the most lethal and untreatable human tumors with a very poor survival rate (up to 18 months). Thus, novel and effective strategies of treatment are still required since resistance and metastasis are major problems of anticancer chemotherapy. Interestingly, ABC transporters, which play a crucial role in the development of multidrug resistance, are modulated by phenothiazine derivatives, while cancer metastasis, migration, and invasion are regulated by cadherins, α-tubulin, and integrins. Methods: The impact on the motility of human glioblastoma U87-MG was performed by wound healing assay, cellular migration and invasion were performed by transwell assay, while ABCB1, ABCG2, E-cadherin, α-tubulin, and integrins (α3, α5, and β1) content were determined by Western blot.Results: The present study explores the effect of perphenazine and prochlorperazine on ABCB1, ABCG2, E-cadherin, α-tubulin, and integrins (α3, α5, and β1) amount as well as migration and invasion ability of human glioblastoma (U87-MG) cells suggesting that phenothiazine derivatives impair multidrug resistance proteins (ABCB1 and ABCG2), E-cadherin, α-tubulin, and integrins amount as well as impair migration and invasion of the U87-MG cell line. Conclusions: The study demonstrated that an increase of ABCG2 and E-cadherin as well as a decrease of α-tubulin, and integrins amount may explain the decrease of migration and invasion ability after phenothiazine derivatives treatment. Moreover, only prochlorperazine significantly reduces the rate of cell migration. Thus, the drug may be considered for the development of new and effective glioblastoma therapy.
Lipid Emulsion And Isoflurane: The Quality Assessment of Anesthesia Parameters In Pigeons
Authors: Ghazanfariasl Alireza , Shahroozian Ebrahim , Keramati Keyvan , Moslemi Hamidreza
Published: September 2021

An objective was to evaluate the efficacy of intravenous (IV) emulsified isoflurane formulation for maintenance of general anesthesia and to compare with IV lipid emulsion infusion with inhalation isoflurane in pigeons. The animals was total of 21 healthy, mature pigeons (Columba livia domestica), weighing 318 ± 13 g. Pigeons were anesthetized by emulsified isoflurane (treatment IΙΙ), inhalation isoflurane with IV lipid emulsion (treatment ΙΙ ), and inhalation isoflurane (treatment Ι) alone. Over 50 minutes, wing tone, toe pinch (pedal), and feather pluck reflex were tested every 10 minutes. Data was recorded at 10, 20 and 30 minutes for temperature (T), peripheral hemoglobin oxygen saturation (SpO2) heart rate (HR), and respiratory rate (fR). A scoring system was used to assess parameters related to anesthesia duration and depth. There were no significant differences in hemodynamic variables between the treatment Ι and treatments ΙΙ and IΙΙ, in treatments associated with fat emulsion have shown faster induction, longer anesthesia, more immobilization, and longer recovery time. Furthermore, in anesthesia depth percentages evaluation it was observed that emulsified isoflurane entered the anesthesia deep stage earlier and was removed immediately after discontinuation of administration.Administration of 8% v/v emulsified isoflurane IV was effective in anesthesia rapid induction, stability in depth of anesthesia, rapid withdrawal from anesthesia depth by discontinuation of the infusion, delayed recovery, cardiorespiratory and (T) stability.
LncRNA FOXD2-AS1 Protects Against Cerebral Ischemia-Reperfusion Injury Through miR-3144-5p/ KCTD15 Axis
Authors: Gao Qi , Wang Yanfeng
Published: September 2021

Background: Long non-coding RNAs (lnc-RNAs) and microRNAs (miRNAs) play key roles in the development of stroke. LncRNA FOXD2-AS1 has been reported to be important in many cancers. However, the role of lncRNA FOXD2-AS1 in stroke is limited known.Methods: Real-time polymerase chain reaction (real-time PCR) assays were used to measure expressions of lncRNA FOXD2-AS1, miR-3144-5p and KCTD15. Western blot assays were employed to examine Bax and Bcl2 protein expression. The cell viability was measured by cck8 assay. The cell apoptosis was detected by TUNEL staining assay The interaction between FOXD2-AS1, miR-3144-5p and KCTD was confirmed by site-directed mutagenesis and luciferase assays. Results: The expression of lncRNA FOXD2-AS1 was down-regulated in blood sample from stroke patients and MAO mice tissues. In addition, lncRNA FOXD2-AS1 was decreased on OGD/R treated cells. LncRNA FOXD2-AS1 overexpression promoted cell viability and reduced apoptosis in OGD/R-Induced PC12 Cells. LncRNA FOXD2-AS1 could interact with miR-3144-5p. Meanwhile, inhibition of miR-3144-5p alleviated OGD/ R-induced neuronal injury in PC12 Cells. Dual luciferase reporter assay verified that KCTD15 was a target of miR-3144-5p. And level of KCTD15 was positive with LncRNA FOXD2-AS1.Conclusion: Taken together, lncRNA FOXD2-AS1 protected against c cerebral ischemia-reperfusion injury by acting as a sponge of miR-3144-5p to modulate KCTD15 level.