bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Teachers’ Judgments of Quality to Establish Performance Standards in Technology Education Across Schools, Communities, and Nations
Authors: Seery Niall , Kimbell Richard , Buckley Jeffrey
Published: March 2022

The establishment and maintenance of national examination standards remains a serious issue for teachers and learners, whilst the levers of control remain firmly in the hands of Awarding Bodies and supervising politicians. Significantly, holistic assessment presents an agility and collective approach to establishing in the minds of teachers “what is of value” when determining the comparative evidence of pupil performance. It is argued in this paper that the collation of the comparative judgment process can initially identify and subsequently maintain standards of performance that can be defined on a cluster, regional or even national level. Much comparative judgment research centers on the formative benefits for learners, but here we place the focus on teachers operating in collaborative groups to establish standards within and beyond their own schools, and ultimately across the nation. We model a proof-of-concept research project. A rank is produced by the collective consensus of the participating teachers and used to simulate a definition of standard. Extrapolations are statistically modeled to demonstrate the potential for this approach to establishing a robust definition of national standards. But central to the process is what is going on in the minds of teachers as they make their judgements of quality. The research aims to draw out teachers’ constructs of quality; to make them explicit; to share them across classrooms and schools; and to empower teachers to debate and agree their standards across schools. This research brings to the fore the symbiotic relationship between teaching, learning and assessment.
Value of Contrast-Enhanced Ultrasound in Mummified Thyroid Nodules
Authors: Chen Sijie , Tang Kui , Gong Yi , Ye Fei , Liao Liyan , Li Xiaodu , Zhang Qi , Xu Yan , Zhang Rongsen , Niu Chengcheng
Published: March 2022

Mummified thyroid nodules (MTNs) are rarely reported and are usually misdiagnosed as malignant nodules. This article first reviewed the contrast-enhanced ultrasound (CEUS) enhancement features of 218 MTNs and classified them into three (A, B, C) patterns. The A pattern MTNs show linear hypo-enhancement, the B pattern MTNs show heterogeneous hypo-enhancement, and the C pattern MTNs show no enhancement in thyroid nodules. The A and C pattern enhancements of MTNs demonstrated a high specificity compared with the enhancement of previously reported typical papillary thyroid carcinomas (PTCs). To further study the B pattern MTNs, 24 B pattern MTNs and 42 PTCs were enrolled in this study, and CEUS parameters for each nodule were evaluated. Univariate analysis indicated that compared with PTCs, the B pattern MTNs more frequently exhibited heterogeneous hypo-enhancement and clear margins after clearance (p <0.05). A multivariate analysis revealed that heterogeneous hypo-enhancement and clear margins after clearance were independent characteristics related to the B pattern MTNs for differentiating them from PTCs (p <0.05). Thus, preoperative CEUS may provide more important information for distinguishing MTNs from malignant thyroid nodules to avoid surgical excisions or unnecessary fine-needle aspiration (FNA).
Systematic Review of Deep Learning and Machine Learning for Building Energy
Authors: Ardabili Sina , Abdolalizadeh Leila , Mako Csaba , Torok Bernat , Mosavi Amir
Published: March 2022

The building energy (BE) management plays an essential role in urban sustainability and smart cities. Recently, the novel data science and data-driven technologies have shown significant progress in analyzing the energy consumption and energy demand datasets for a smarter energy management. The machine learning (ML) and deep learning (DL) methods and applications, in particular, have been promising for the advancement of accurate and high-performance energy models. The present study provides a comprehensive review of ML- and DL-based techniques applied for handling BE systems, and it further evaluates the performance of these techniques. Through a systematic review and a comprehensive taxonomy, the advances of ML and DL-based techniques are carefully investigated, and the promising models are introduced. According to the results obtained for energy demand forecasting, the hybrid and ensemble methods are located in the high-robustness range, SVM-based methods are located in good robustness limitation, ANN-based methods are located in medium-robustness limitation, and linear regression models are located in low-robustness limitations. On the other hand, for energy consumption forecasting, DL-based, hybrid, and ensemble-based models provided the highest robustness score. ANN, SVM, and single ML models provided good and medium robustness, and LR-based models provided a lower robustness score. In addition, for energy load forecasting, LR-based models provided the lower robustness score. The hybrid and ensemble-based models provided a higher robustness score. The DL-based and SVM-based techniques provided a good robustness score, and ANN-based techniques provided a medium robustness score.
Risk Assessment of AC/DC Hybrid Distribution Network considering New Energy and Electric Vehicle Access
Authors: Dong Jie , Zhao Jianjun , Liu Ziyue , Li Yang , Yu Zhongshu , Liu Jialin , Wang Zhenhao
Published: March 2022

In view of the operational risk issues such as safety and economy caused by the connection of new energy and multiple types of electric vehicles (EVs) to the AC–DC distribution network, an AC–DC distribution network operational risk assessment method that takes into account multiple risk factors is proposed. First, a probability distribution model of scenery output and EV timing is constructed, and the starting charge state of multiple types of EVs is replaced by the number of daily driving miles subjectively set; then, based on the complex network theory, timing safety indicators, such as voltage overrun risk and branch power overload operation risk, are proposed, and the economic risk is established according to the economic operation of the distribution grid; Furthermore, a risk assessment matrix for grid-connected EVs with different capacities is constructed, and the principal component analysis (PCA) method is used to reduce the dimension of the risk assessment matrix and calculate the objective weight coefficient; finally, taking the improved IEEE 33 node AC/DC power distribution system as an example, the comprehensive risk evaluation based on PCA is compared with the traditional one, and the results show that when the safety and economic risk factors are considered at the same time, the operation risk in a certain range has a downward trend when the proposed method is adopted, which has a positive guiding significance for the planning of EV capacity in a certain area.
Two-Stage Optimal Location Allocations of DPFC Considering Wind and Load Uncertainty
Authors: Zhu Xuedong , Dichen Liu , Wu Jun
Published: March 2022

Distributed power flow controller (DPFC) has a considerable potential to regulate the power flow and generator rescheduling continuously. This study presents a novel two-stage stochastic model for optimal location allocations of the DPFC coupled with the interactions of DPFC to search for the optimal solutions. The Benders decomposition is utilized to reformulate the two-stage problem into the master problem and the subproblem. The optimal solution can be easily obtained with the master problem and subproblem iteratively. The relaxed DC power flow with a DPFC in the master problem accelerates the efficiency of optimal locations under a base condition. Slack variables are incorporated in the subproblem to check the feasibility of relaxed AC power flow. The optimal compensation levels of DPFC at different load/wind scenarios are optimized in the subproblem. The IEEE 118 bus system is conducted to verify the performance of the proposed procedure. The DPFC has positive impacts on unit costs, voltage performance, wind absorption, and power losses. Detailed simulation results illustrate the effect of the proposed approach.
Representation of Drought Events in the United Kingdom: Contrasting 200 years of News Texts and Rainfall Records
Authors: Dayrell Carmen , Svensson Cecilia , Hannaford Jamie , McEnery Tony , Barker Lucy J. , Baker Helen , Tanguy Maliko
Published: March 2022

This paper combines evidence from the analyses of large sets of newspaper material and long-term rainfall records to gain insights into representations of drought events in the United Kingdom, between 1800 and 2014. More specifically, we bring together two different, though complementary, approaches to trace longitudinal patterns in the ways drought events have been measured and perceived, focusing specifically on the duration, spatial extent, and intensity of each event. The power of the combined approach is demonstrated through three case studies (1911–1913, 1940–1945, and 1947–1949), in which we explore the available evidence in more detail and explore the impacts of the droughts upon the British population. Using corpus linguistics methods, we examined four sets of newspaper material: 1) the British Library 19th century newspaper corpus, 2) The Times 20th century corpus (both i and ii with over five billion words), 3) 4,986 texts from British broadsheet papers (3.8 million words) and 4) 2,384 texts from tabloids (1.1 million words). An independent analysis of meteorological drought was undertaken using three sources: 1) the England and Wales Precipitation (EWP) series (back to 1800), 2) a statistically reconstructed version of the EWP which is more reliable in the early record (pre-1870), and 3) a high resolution gridded dataset (back to 1862) which is aggregated to NUTS1 regional scales. Meteorological droughts were assessed using the Standardised Precipitation Index, which allowed us to appraise drought intensity, severity and duration. We found an overwhelming agreement between the corpus data and meteorological records. For the very few cases of disparity between the corpus and rainfall data, there were in most cases plausible explanations. All in all, the present study demonstrates the power of the combined approach, presenting evidence on a scale that would not otherwise be possible, thus contributing to a better understanding of how drought is perceived, in addition to how it is traditionally “measured”.
Using Tencent User Location Data to Modify Night-Time Light Data for Delineating Urban Agglomeration Boundaries
Authors: He Xiong , Zhu Yiting , Chang Pengpeng , Zhou Chunshan
Published: March 2022

The study of urban agglomeration boundaries is helpful to understand the internal spatial structure of urban agglomeration, evaluate the development level of urban agglomeration, and thus, assist in the formulation of regional planning and policies. However, previous studies often used only static spatial elements to delineate the boundaries of urban agglomerations, ignoring the spatial connections within urban agglomerations. In this study, night-time light and Tencent user location data were evaluated separately and fused to delineate urban agglomeration boundaries from both static and dynamic spatial perspectives. Additionally, it has been shown in the study results that the accuracy of urban agglomeration boundary delineated by night-time light data is 84.90%, with Kappa coefficient as 0.6348. The accuracy delineated by Tencent user location data is 82.40%, with Kappa coefficient as 0.5637, while the accuracy delineated by data fusion is 92.70%, with Kappa coefficient as 0.7817. Therefore, it can be concluded that the fusion of night-time light and Tencent user location data had the highest accuracy in delineating urban agglomeration boundaries, which verified that the fusion of dynamic spatial elements on a single static spatial element can supplement the spatial connection of urban agglomeration. Our findings enrich the understanding of urban agglomerations, and the accurate delineation of urban agglomerations boundaries can aid urban agglomeration planning and management.
Bison Reintroduction to Mixed-Grass Prairie Is Associated With Increases in Bird Diversity and Cervid Occupancy in Riparian Areas
Authors: Boyce Andy J. , Shamon Hila , McShea William J.
Published: March 2022

In grassland ecosystems, grazing by large herbivores is a highly influential process that affects biodiversity by modifying the vegetative environment through selective consumption. Here, we test whether restoration of bison is associated with increased bird diversity and cervid occupancy in networks of riparian habitat within a temperate grassland ecosystem, mixed-grass prairie in northcentral Montana, United States. We used a long time-series of remote sensing imagery to examine changes in riparian vegetation structure in stream networks within bison and cattle pastures. We then assessed how vegetation structure influenced diversity of bird communities and detection rates of mammals in these same riparian networks. We found that percent cover of woody vegetation, and native grasses and forbs increased more rapidly over time in bison pastures, and that these changes in vegetation structure were associated with increased bird diversity and cervid occupancy. In conclusion, bison reintroduction appears to function as a passive riparian restoration strategy with positive diversity outcomes for birds and mammals.
A Bibliometric and Knowledge-Map Analysis of CAR-T Cells From 2009 to 2021
Authors: Miao Lele , Zhang Juan , Zhang Zhengchao , Wang Song , Tang Futian , Teng Muzhou , Li Yumin
Published: March 2022

Objectives

A bibliometric and knowledge-map analysis is used to explore hotspots’ evolution and development trends in the CAR-T cell field. By looking for research hotspots and new topics, we can provide new clues and ideas for researchers in this field.

Methods

The articles and reviews regarding CAR-T cells were retrieved and obtained from the Web of Science Core Collection (WOSCC) on October 28th, 2021. CtieSpace [version 5.8.R3 (64-bit)] and VOSviewer (version 1.6.17) were used to conduct the bibliometric and knowledge-map analysis.

Results

660 authors from 488 institutions in 104 countries/regions published 6,867 papers in 1,212 academic journals. The United States was absolutely in the leading position in this research field. The institution that contributed the most publications was the University of Pennsylvania. Carl H June published the most articles, while Shannon L Maude had the most co-citations. However, there was little cooperation between countries. After 2012, cooperation among various institutions was also small. The journals that published the most CAR-T cell-related papers were Frontiers in immunology and Cancers. Nevertheless, Blood and The New England Journal of Medicine were the most commonly co-cited journals. The most influential research hotspots were the research of CAR-T cells in hematological malignancies, the related research of cytokine release syndrome (CRS), CD19, and the anti-tumor activity and efficacy of CAR-T cells. The latest hotspots and topics included the study of CAR-T cells in solid tumors, universal CAR-T cells, CAR-NK cells, CD22, and anakinra (the IL-1 receptor antagonist). The research of CAR-T cells in solid tumors was a rapidly developing hot field. Emerging topics in this field mainly included the study of CAR-T cells in glioblastoma (related targets: IL13Rα2, EGFRvIII, and HER2), neuroblastoma (related target: GD2), sarcoma (related target: HER2), and pancreatic cancer (related target: mesothelin), especially glioblastoma.

Conclusion

As an anti-tumor therapy with great potential and clinical application prospects, CAR-T cell therapy is still in a stage of rapid development. The related field of CAR-T cells will remain a research hotspot in the future.
Integrated Bioinformatics and Validation Reveal IL1B and Its Related Molecules as Potential Biomarkers in Chronic Spontaneous Urticaria
Authors: Peng Shixiong , Zhang Teng , Zhang Sisi , Tang Qian , Yan Yang , Feng Hao
Published: March 2022

Background

The etiopathogenesis of chronic spontaneous urticaria (CSU) has not been fully understood, and there has been extensive interest in the interaction between inflammatory dermatosis and pyroptosis. This study intends to investigate the molecular mechanism of pyroptosis-related genes in CSU via bioinformatic ways, aiming at identifying the potential key biomarker.

Methods

GSE72540, the RNA expression profile dataset of CSU, was utilized as the training set, and GSE57178 as the validation set. Differently expressed pyroptosis-related genes (DEPRGs), GO, KEGG, and DO analyses were performed. The hub genes were explored by the protein–protein interaction analysis. Moreover, CIBERSORT was employed for estimating immune cell types and proportions. Then, we constructed a DEmRNA–miRNA–DElncRNA ceRNA network and a drug–gene interaction network. Finally, ELISA was used for gene expression analysis.

Results

We recognized 17 DEPRGs, whose enrichment analyses showed that they were mostly enriched in inflammatory response and immunomodulation. Moreover, 5 hub genes (IL1B, TNF, and IRF1 are upregulated, HMGB1 and P2RX7 are downregulated) were identified via the PPI network and verified by a validation set. Then immune infiltration analysis displayed that compared with normal tissue, CSU owned a significantly higher proportion of mast cells activated, but a lower proportion of T cells CD4 naive and so on. Furthermore, IL1B was statistically and positively associated with mast cells activated in CSU, and SNHG3, the upstream factor of IL1B in the ceRNA we constructed, also related with mast cells in CSU. Further analysis exhibited that the protein subcellular localization of IL1B was extracellular, according with its intercellular regulation role; IL1B was significantly correlated with key immune checkpoints; and the NOD-like receptor signaling pathway was the mainly involved pathway of IL1B based on the couple databases. What is more, the result of ELISA of CSU patients was the same as the above analyses about IL1B. In addition, the drug–gene interaction network contained 15 potential therapeutic drugs targeting IL1B, and molecular docking might make this relationship viable.

Conclusion

IL1B and its related molecules might play a key role in the development of CSU and could be potential biomarkers in CSU.
Dietary Probiotic Supplementation Suppresses Subclinical Necrotic Enteritis in Broiler Chickens in a Microbiota-Dependent Manner
Authors: Zhao Ying , Zeng Yan , Zeng Dong , Wang Hesong , Sun Ning , Xin Jinge , Zhou Mengjia , Yang Hanbo , Lei Lei , Ling Hongli , Khalique Abdul , Rajput Danish Sharafat , Gan Baoxing , Wan Zhiqiang , Yao Zhipeng , Fang Jing , Pan Kangcheng , Shu Gang , Jing Bo , Zhang Dongmei , Ni Xueqin
Published: March 2022

Background

Chicken meat is one of the most consumed meats worldwide and poultry production is increasing at an exponential rate. Reducing antibiotic usage has resulted in the recurrence of subclinical necrotic enteritis again and influenced global poultry production. Probiotics are potential antibiotic substitutes that can be used to prevent subclinical necrotic enteriti. However, the precise mechanism of action of probiotics and information on which gut microbes confer this efficacy remain elusive.

Methods and results

The subclinical necrotic enteritis animal model was used to reveal the mechanism underlying the effect of probiotics on intestinal health through RNA sequencing and 16S rDNA amplicon sequencing. Bacillus licheniformis H2 feeding significantly reduced the relative abundance of Clostridium perfringens in the ileum and markedly ameliorated the pathological damage in the ileum and liver. In addition, oral administration of B. licheniformis H2 contributed to the enhancement of the intestinal barrier function and epithelial renewal, reducing energy consumption, and improving enteral nutrition absorption. Probiotic B. licheniformis H2 also ameliorated the inflammatory response and increased the immunity of subclinical necrotic enteritis infected broilers. Finally, B. licheniformis H2 feeding regulated liver gene expression to suppress immune response and promoted growth and metabolism depending on the gut microbiota.

Conclusions

These results indicated the mechanism of probiotic action of B. licheniformis H2 in maintaining intestinal health and thus promoting growth and B. licheniformis H2 may serve as an antibiotic substitute to prevent subclinical necrotic enteritis in poultry farming.
Chronic Alcohol Exposure Among People Living with HIV Is Associated with Innate Immune Activation and Alterations in Monocyte Phenotype and Plasma Cytokine Profile
Authors: Underwood Michelle L. , Park Byung , Uebelhoer Luke S. , Gu Geoffrey , Kunkel Lynn E. , Korthuis Philip T. , Cook Ryan R. , Sekaly Rafick Pierre , Ribeiro Susan Pereira , Lancioni Christina L.
Published: March 2022

Despite advances in antiretroviral therapy, chronic immune activation continues to be observed among individuals with well-controlled HIV viral loads, and is associated with non-AIDS defining morbidities among people living with HIV. Alcohol use disorder impacts a significant proportion of individuals living with HIV, and alcohol exposure is known to damage the intestinal epithelium which may increase translocation of pathogens and their molecular products, driving systemic immune activation and dysregulation. The aim of this study was to determine if adults living with HIV with well-controlled viral loads, who also suffer from alcohol use disorder with and without hepatitis C virus co-infection (n=23), exhibit evidence of advanced systemic immune activation, intestinal damage, and microbial translocation, as compared to adults living with HIV who are not exposed to chronic alcohol or other substances of abuse (n=29). The impact of a 1-month intervention to treat alcohol-use disorder was also examined. Alcohol-use disorder was associated with evidence of advanced innate immune activation, alterations in monocyte phenotype including increased expression of Toll-like receptor 4, increased burden of stimulatory ligands for Toll-like receptor 4, and alterations in plasma cytokine signature, most notably elevations in soluble CD40 ligand and transforming growth factor beta. Alcohol-associated immune activation was more pronounced among individuals with hepatitis C virus co-infection. Although the 1-month intervention to treat alcohol use disorder did not result in significant reductions in the interrogated indicators of immune activation, our findings suggest that chronic alcohol exposure is a major modifiable risk factor for chronic immune activation and dysregulation among people-living with HIV.
Live-Attenuated VEEV Vaccine Delivered by iDNA Using Microneedles Is Immunogenic in Rabbits
Authors: Tretyakova Irina , Tomai Mark , Vasilakos John , Pushko Peter
Published: March 2022

Effective and simple delivery of DNA vaccines remains a key to successful clinical applications. Previously, we developed a novel class of DNA vaccines, sometimes called iDNA, which encodes the whole live-attenuated vaccine viruses. Compared to a standard DNA vaccine, an iDNA vaccine required a low dose to launch a live-attenuated vaccine in vitro or in vivo. The goal of this pilot study was to investigate if iDNA vaccine encoding live-attenuated Venezuelan equine encephalitis virus (VEEV) can be efficiently delivered in vivo by a microneedle device using a single-dose vaccination with naked iDNA plasmid. For this purpose, we used pMG4020 plasmid encoding live-attenuated V4020 vaccine of VEE virus. The V4020 virus contains structural gene rearrangement, as well as attenuating mutations genetically engineered to prevent reversion mutations. The pMG4020 was administered to experimental rabbits by using a hollow microstructured transdermal system (hMTS) microneedle device. No adverse events to vaccination were noted. Animals that received pMG4020 plasmid have successfully seroconverted, with high plaque reduction neutralization test (PRNT) antibody titers, similar to those observed in animals that received V4020 virus in place of the pMG4020 iDNA plasmid. We conclude that naked iDNA vaccine can be successfully delivered in vivo by using a single-dose vaccination with a microneedle device.
From Embryo to Adult Life: Differential Expression of Visual Opsins in the Flatfish Solea senegalensis Under Different Light Spectra and Photoperiods
Authors: Frau Sara , Paullada-Salmerón José A. , Paradiso Isabella , Cowan Mairi E. , Martín-Robles Águeda J. , Muñoz-Cueto José A.
Published: March 2022

Visual photoreceptors in fish are usually adjusted to the light environment to ensure the highest efficiency and best adaptation. In the Senegalese sole, metamorphosis determines migration from pelagic to benthic habitats, with marked differences in both light intensity and spectrum. Here, we analysed the ontogeny of six visual photopigments, namely, rod opsin (rh1), short wavelength-sensitive (sws1 and sws2), medium wavelength-sensitive (rh2.3 and rh2.4), and long wavelength-sensitive (lws) cone opsins, in sole specimens maintained in light-dark cycles of white (LDW), blue (LDB), red (LDR), and continuous white (LL) lights by using RT-qPCR and in situ hybridisation. Most of the opsins displayed a similar developmental expression pattern under all tested conditions. However, lower transcripts were detected under LDR and LL compared to LDW and LDB. A significant increase in gene expression was detected before and after metamorphosis, reaching minimum transcript levels at hatching and during metamorphosis. Interestingly, green opsins (rh2.3 and rh2.4) displayed a significant increase only before metamorphosis, with their expression remaining low during and after metamorphosis. The rod opsin and short-, medium-, and long-wavelength sensitive cone opsins were detected in retinal photoreceptors of the sole from pre-metamorphic to adult stages by in situ hybridisation. In adults, the short-wavelength cone opsins (sws1 and sws2) were found in single cones, whereas the medium- (rh2.4) and long-wavelength (lws) cone opsins were present in double cones. The results obtained by in situ hybridisation in the retina of developing sole, in terms of number of positive cells and/or intensity of labelling, were consistent with the ontogenetic transcript patterns found by RT-qPCR, suggesting that most of the visual opsin expressions detected in the whole specimens could correspond to retinal expression. Taken together, our results pointed out that the ontogeny of the Senegalese sole is accompanied by remodelling in opsin gene expression, with the green-cone opsins being the most abundant photopigments in pre-metamorphosis and rod opsin the dominant visual photopigment from the completion of metamorphosis onwards. These results enlarge our knowledge of flatfish metamorphosis and ecology and provide useful information to develop light protocols adapted to different ontogenetic stages that could improve welfare and production in sole aquaculture.
Comparative Analysis of Physiological Responses in Two Ulva prolifera Strains Revealed the Effect of Eutrophication on High Temperature and Copper Stress Tolerance
Authors: Bao Menglin , Park Ji-Sook , Xing Qikun , He Peimin , Zhang Jianheng , Yarish Charles , Yoo Hyun Il , Kim Jang K.
Published: March 2022

Climate change directly influences species composition and distribution of macroalgae on a global scale. Meanwhile, eutrophication and heavy metal pollution continue to be major concerns in coastal areas. The objective of this study was to explore interactive effects of these complex environmental conditions (high temperature, eutrophication and heavy metal pollution) in the bloom forming green alga, Ulva prolifera. This study evaluated the physiological characteristics of two strains of U. prolifera (Korean strain and Chinese strain) at two temperature levels (20 and 25°C), two nutrient concentrations (low nutrient: 50 μM of N and 5 μM of P; high nutrient: 250 μM of N and 25 μM of P) and three copper concentrations [Control (0.03 μM); 0.1 μM; 1 μM]. Under the 20°C and low nutrient condition, the Chinese strain grew significantly slower at 1μM copper group compared to copper control group. Whereas there was no significant change in the growth rate of the Korean strain. High nutrient significantly increased the growth rate, photosynthetic and respiration rates, soluble protein and tissue N in both strains at all copper groups under 20°C compared to copper groups under 20°C and low nutrient condition. Both strains grew significantly slower at 1 μM copper group compare to copper control group under high nutrient and 20°C condition. 25°C significantly reduced the growth rate of both strains at each nutrient condition. Under 25°C and low nutrient condition, 1 μM copper had no effects on growth rate and all photosynthetic parameters of both strains. Meanwhile, under 25°C and high nutrient conditions, 1 μM copper significantly inhibited growth and photosynthetic rates and increased soluble protein content in the Chinese strain compared to copper control treatment. These results suggest that the Korean strain had higher tolerance to the copper stress than the Chinese strain. Higher nutrient levels enhanced the temperature tolerance and the copper stress in U. prolifera. Increased temperature reduced the negative effects caused by copper stress in U. prolifera.
Psychotropic Medication Use Is Associated With Greater 1-Year Incidence of Dementia After COVID-19 Hospitalization
Authors: Freudenberg-Hua Yun , Makhnevich Alexander , Li Wentian , Liu Yan , Qiu Michael , Marziliano Allison , Carney Maria , Greenwald Blaine , Kane John M. , Diefenbach Michael , Burns Edith , Koppel Jeremy , Sinvani Liron
Published: March 2022

Background

COVID-19 has been associated with an increased risk of incident dementia (post-COVID dementia). Establishing additional risk markers may help identify at-risk individuals and guide clinical decision-making.

Methods

We investigated pre-COVID psychotropic medication use (exposure) and 1-year incidence of dementia (outcome) in 1,755 patients (≥65 years) hospitalized with COVID-19. Logistic regression models were used to examine the association, adjusting for demographic and clinical variables. For further confirmation, we applied the Least Absolute Shrinkage and Selection Operator (LASSO) regression and a machine learning (Random Forest) algorithm.

Results

One-year incidence rate of post-COVID dementia was 12.7% (N = 223). Pre-COVID psychotropic medications (OR = 2.7, 95% CI: 1.8–4.0, P < 0.001) and delirium (OR = 3.0, 95% CI: 1.9–4.6, P < 0.001) were significantly associated with greater 1-year incidence of post-COVID dementia. The association between psychotropic medications and incident dementia remained robust when the analysis was restricted to the 423 patients with at least one documented neurological or psychiatric diagnosis at the time of COVID-19 admission (OR = 3.09, 95% CI: 1.5–6.6, P = 0.002). Across different drug classes, antipsychotics (OR = 2.8, 95% CI: 1.7–4.4, P < 0.001) and mood stabilizers/anticonvulsants (OR = 2.4, 95% CI: 1.39–4.02, P = 0.001) displayed the greatest association with post-COVID dementia. The association of psychotropic medication with dementia was further confirmed with Random Forest and LASSO analysis.

Conclusion

Confirming prior studies we observed a high dementia incidence in older patients after COVID-19 hospitalization. Pre-COVID psychotropic medications were associated with higher risk of incident dementia. Psychotropic medications may be risk markers that signify neuropsychiatric symptoms during prodromal dementia, and not mutually exclusive, contribute to post-COVID dementia.
Fusobacterium nucleatum Affects Cell Apoptosis by Regulating Intestinal Flora and Metabolites to Promote the Development of Colorectal Cancer
Authors: Yu Tingting , Ji Ling , Lou Liqin , Ye Shiqing , Fang Xiaoting , Li Chen , Jiang Feizhao , Gao Hongchang , Lou Yongliang , Li Xiang
Published: March 2022

Background/Aims

Intestinal flora, especially Fusobacterium nucleatum (Fn), can affect the development of colorectal cancer (CRC). In this study, we examined the composition of intestinal flora and their metabolites in the tissues, serum and feces of CRC patients.

Materials and Methods

CRC tissues, adjacent normal colonic tissues, fecal and serum samples were collected from CRC patients who received surgical treatment between January 2018 and January 2020. Fecal and serum samples were collected from healthy individuals for comparison. In addition, fecal samples were collected from BALB/c female mice. SW480, a human CRC cell line, was utilized for in vitro studies. The experiments involved 1H-NMR-based metabolomics analysis, targeted and untargeted mass spectrometry analysis, and intestinal flora 16S rDNA V4 region sequencing.

Results

The abundance of Bacteroides and propionic acid concentration were decreased and that of Lactobacillus and lactic acid concentration were increased in CRC tissues. In addition, the abundances of Ruminococcus, Prevotella, and Sutterell were decreased in CRC patients. The levels of leucine and isoleucine were decreased in the serum and tumor tissues of CRC patients. Aspartate, glutamate and glutathione levels were elevated in the tissues of CRC patients only. The serum glutamine, tyrosine, valine, alanine, and histidine levels were decreased significantly. Lactic acid inhibited and propionic acid promoted apoptosis among SW480 CRC cells.

Conclusion

Fn affected the apoptosis of CRC cells and promoted the progression of CRC by affecting the distribution of intestinal flora, which altered the concentrations of metabolites such as lactic acid, propionic acid. Intestinal flora could regulate amino acid metabolism.
Profound Change in Soil Microbial Assembly Process and Co-occurrence Pattern in Co-inoculation of Bradyrhizobium japonicum 5038 and Bacillus aryabhattai MB35-5 on Soybean
Authors: Zhao Yubin , Guan Dawei , Liu Xu , Gao Gui-Feng , Meng Fangang , Liu Bingqiang , Xing Pengfei , Jiang Xin , Ma Mingchao , Cao Fengming , Li Li , Li Jun
Published: March 2022

Rhizosphere microbial communities are vital for plant growth and soil sustainability; however, the composition of rhizobacterial communities, especially the assembly process and co-occurrence pattern among microbiota after the inoculation of some beneficial bacteria, remains considerably unclear. In this study, we investigated the structure of rhizomicrobial communities, their assembly process, and interactions contrasting when Bradyrhizobium japonicum 5038 and Bacillus aryabhattai MB35-5 are co-inoculated or Bradyrhizobium japonicum 5038 mono-inoculated in black and cinnamon soils of soybean fields. The obtained results indicated that the Chao and Shannon indices were all higher in cinnamon soil than that in black soil. In black soil, the co-inoculation increased the Shannon indices of bacteria comparing with that of the mono-inoculation. In cinnamon soil, the co-inoculation decreased the Chao indices of fungi comparing with that of mono-inoculation. Compared with the mono-inoculation, the interactions of microorganisms of co-inoculation in the co-occurrence pattern increased in complexity, and the nodes and edges of co-inoculation increased by 10.94, 40.18 and 4.82, 16.91% for bacteria and fungi, respectively. The co-inoculation of Bradyrhizobium japonicum 5038 and Bacillus aryabhattai MB35-5 increased the contribution of stochastic processes comparing with Bradyrhizobium japonicum 5038 inoculation in the assembly process of soil microorganisms, and owing to the limitation of species diffusion might restrict the direction of pathogenic microorganism movement. These findings support the feasibility of rebuilding the rhizosphere microbial system via specific microbial strain inoculation and provide evidence that the co-inoculation of Bradyrhizobium japonicum 5038 and Bacillus aryabhattai MB35-5 can be adopted as an excellent compound rhizobia agent resource for the sustainable development of agriculture.
Intercropping Walnut and Tea: Effects on Soil Nutrients, Enzyme Activity, and Microbial Communities
Authors: Bai Yong-Chao , Li Bao-Xin , Xu Chun-Yong , Raza Mubashar , Wang Qi , Wang Qi-Zhu , Fu Ya-Nan , Hu Jian-Yang , Imoulan Abdessamad , Hussain Muzammil , Xu Yong-Jie
Published: March 2022

The practice of intercropping, which involves growing more than one crop simultaneously during the same growing season, is becoming more important for increasing soil quality, land-use efficiency, and subsequently crop productivity. The present study examined changes in soil physicochemical properties, enzymatic activity, and microbial community composition when walnut (Juglans spp.) was intercropped with tea (Camellia sinensis L.) plants in a forest and compared with a walnut and tea monocropping system. The results showed that walnut–tea intercropping improved the soil nutrient profile and enzymatic activity. The soil available nitrogen (AN), available phosphorus (AP), available potassium (AK), organic matter (OM) content, and sucrase activity were significantly boosted in intercropped walnut and tea than in monocropping forests. The interaction between crops further increased bacterial and fungal diversity when compared to monoculture tea forests. Proteobacteria, Bacteroidetes, Firmicutes, Chlamydiae, Rozellomycota, and Zoopagomycota were found in greater abundance in an intercropping pattern than in monoculture walnut and tea forest plantations. The walnut–tea intercropping system also markedly impacted the abundance of several bacterial and fungal operational taxonomic units (OTUs), which were previously shown to support nutrient cycling, prevent diseases, and ameliorate abiotic stress. The results of this study suggest that intercropping walnut with tea increased host fitness and growth by positively influencing soil microbial populations.
The Usage of Histamine Type 1 Receptor Antagonist and Risk of Dementia in the Elderly: A Nationwide Cohort Study
Authors: Yang Chuan-Chi , Chien Wu-Chien , Chung Chi-Hsiang , Lai Chung-Yu , Tzeng Nian-Sheng
Published: March 2022

Background

The histamine type 1 receptor antagonist (H1RA) has been commonly used. This study aimed to examine the association between the usage of H1RA and the risk of dementia.

Methods

A total of 8,986 H1RA users aged ≥50 and 26,958 controls matched a ratio of 1:3 for age, sex, and comorbidity, were selected between January 1, and December 31, 2000, from Taiwan’s National Health Insurance Research Database. Fine and Gray’s survival analysis (competing with mortality) was used to compare the risk of developing dementia during a 15-year follow-up period (2000–2015).

Results

In general, the H1RA usage was not significantly associated with dementia (adjusted subdistribution hazard ratio [SHR] = 1.025, 95% confidence interval [CI] = 0.883–1.297, p = 0.274) for the H1RA cohort. However, a differential risk was found among the groups at risk. The patients with the usage of H1RA aged ≥65 years (adjusted SHR: 1.782, 95% CI = 1.368–2.168, p < 0.001) were associated with a higher risk of dementia, in comparison to the control groups. Furthermore, the patients with the usage of H1RA that were male, or had more comorbidities, were also associated with an increased risk of dementia.

Conclusion

The usage of H1RA was associated with the risk of developing dementia in the patients aged ≥ 65 years.
An Adaptive Time-Varying Impedance Controller for Manipulators
Authors: Liang Xu , Su Tingting , Zhang Zhonghai , Zhang Jie , Liu Shengda , Zhao Quanliang , Yuan Junjie , Huang Can , Zhao Lei , He Guangping
Published: March 2022

Aiming at the situation that the structural parameters of the general manipulators are uncertain, a time-varying impedance controller based on model reference adaptive control (MRAC) is proposed in this article. The proposed controller does not need to use acceleration-based feedback or to measure external loads and can tolerate considerable structure parameter errors. The global uniform asymptotic stability of the time-varying closed-loop system is analyzed, and a selection approach for control parameters is presented. It is demonstrated that, by using the proposed control parameter selection approach, the closed-loop system under the adaptive controller is equivalent to an existing result. The feasibility of the presented controller for the general manipulators is demonstrated by some numerical simulations.
Effects of Optimized Acupuncture and Moxibustion Treatment on Depressive Symptoms and Executive Functions in Patients With Post-Stroke Depression: Study Protocol for a Randomized Controlled Trial
Authors: Luo Meng , Duan Zhaoyuan , Song Xiaolei , Liu Chengmei , Li Ruiqing , Su Kaiqi , Bai Yanjie , Wang Xiaodan , Fu Wenbin , Gao Jing , Feng Xiaodong
Published: March 2022

Background

Post-stroke depression (PSD), a common neuropsychiatric comorbidity after stroke, has a negative impact on the functional recovery and quality of life of survivors. It lacks effective therapeutic drugs with good curative effects and few adverse reactions. Preliminary experiments have shown that the optimized acupuncture and moxibustion treatment (OAMT), including acupuncture, moxibustion, and auricular intradermal acupuncture, improved depressive symptoms and neurological deficits in patients with PSD. However, the evidence for its effectiveness is still insufficient. Hence, we designed this study to evaluate the efficacy and safety of the OAMT in the treatment of PSD and to explore its possible mechanism from the perspective of executive functions.

Methods/Design

This is a randomized controlled trial, which comprises a total of 134 patients with PSD. Participants are randomized into intervention group and control group at a 1:1 ratio. All treatments are given five times per week for 4 weeks. The primary outcome is the severity of depression, which is evaluated by the Hamilton Depression Scale-17 (HAMD-17) and the Beck Depression Rating Scale (BDI). Secondary outcomes are executive abilities, which are measured by several neuropsychological tests, including the Stroop Color and Word Test (SCWT), the Trial Making Test (TMT), the Digit Symbol Substitution Test (DSST), and the Matrix Reasoning Test (MRT). All outcomes have been evaluated at baseline and weeks 4, 8, 12, and 20. At the same time, functional MRI (fMRI) is used to measure the functional connectivity in the cognitive control network (CCN) at baseline and 4 weeks after intervention.

Discussion

This study aims to provide high-quality evidence for the efficacy and safety of the OAMT for treating PSD. In addition, this trial is the first trial to explore if the improvement condition of depression in the OAMT group is related to the improvement of executive functions and the favorable changes in the structure.

Clinical Trial Registration

Chinese Clinical Trial Registry, identifier: ChiCTR2100048431.
Matrix Metalloproteinase-9 as an Important Contributor to the Pathophysiology of Depression
Authors: Li Hongmin , Sheng Zhaofu , Khan Suliman , Zhang Ruiyi , Liu Yang , Zhang Yan , Yong V. Wee , Xue Mengzhou
Published: March 2022

Matrix metalloproteinases (MMPs) are physiologically expressed in the central nervous system in neurons, astrocytes and microglia, and their aberrant elevation contributes to a number of diseases. Amongst the MMP members, MMP−9 has generated considerable attention because of its possible involvement in inflammatory responses, blood-brain barrier permeability, the regulation of perineuronal nets, demyelination, and synaptic long-term potentiation. Emerging evidence indicate an association between MMP−9 and the syndrome of depression. This review provides an updated and comprehensive summary of the probable roles of MMP−9 in depression with an emphasis on the mechanisms and potential of MMP−9 as a biomarker of depression.
Self-Perception and the Relation to Actual Driving Abilities for Individuals With Visual Field Loss
Authors: Andersson Jan , Bro Tomas , Lajunen Timo
Published: March 2022

Background

In Sweden, individuals with visual field loss (VFL) have their driving license withdrawn. The literature clearly indicates that individuals with VFL are unsafe drivers on a group level. However, many drivers with VFL can be safe on an individual level. The literature also suggests that self-perception, beliefs, and insights of one’s own capabilities are related to driving performance. This study had three aims: (1) To investigate self-perceived driving capability ratings for individuals with VFL; (2) to compare these ratings between groups with different medical conditions associated with VFL (stroke, glaucoma, and diabetes); and (3) to relate the self-perception ratings to actual driving performance in an advanced driving simulator.

Methods

Participants comprised 723 individuals whose driver’s license had been withdrawn because of VFL and 92 normally sighted elderly individuals. All participants completed a background survey, rated difficulties with different traffic situations, rated their strengths and weaknesses as drivers, and rated aspects that were important for causing traffic accidents. Of the VFL group participants, 264 also completed a simulator-based driving test that they knew could lead to renewal of their driving license. VFL participants and normally sighted was at the same age when they completed the simulator driving test.

Results

Overall, individuals with VFL rated their capabilities as high on all instruments and scales used, even higher than the elderly normally sighted control group. The only VFL etiology group that rated lower than other groups was the diabetes group. Safety orientation and internal control orientation values were best at discriminating between VFL participants in terms of self-perception of driving performance. Participants categorized as “high” in terms of safety skills and internal control were more modest in their ratings. Finally, participants who passed the simulated driving test did not differ from those who failed, in any of the self-perception measures.

Conclusion

Self-perception ratings among individuals with VFL were higher than those of normally sighted elderly individuals. Self-assessed skills did not predict driving performance. Groups with different VFL etiologies rated similarly. Self-ratings of driving abilities cannot be used to assess actual driving performance. Actual driving tests (on road or in the simulator) are necessary to discriminate between safe and unsafe drivers with VFL.
Photobiomodulation of Cytochrome c Oxidase by Chronic Transcranial Laser in Young and Aged Brains
Authors: Cardoso Fabrízio dos Santos , Barrett Douglas W. , Wade Zachary , Gomes da Silva Sérgio , Gonzalez-Lima F.
Published: March 2022

In cellular bioenergetics, cytochrome c oxidase (CCO) is the enzyme responsible for oxygen consumption in the mitochondrial electron transport chain, which drives oxidative phosphorylation for adenosine triphosphate (ATP) production. CCO is also the major intracellular acceptor of photons in the light wavelengths used for photobiomodulation (PBM). Brain function is critically dependent on oxygen consumption by CCO for ATP production. Therefore, our objectives were (1) to conduct the first detailed brain mapping study of the effects of PBM on regional CCO activity, and (2) to compare the chronic effects of PBM on young and aged brains. Specifically, we used quantitative CCO histochemistry to map the differences in CCO activity of brain regions in healthy young (4 months old) and aged (20 months old) rats from control groups with sham stimulation and from treated groups with 58 consecutive days of transcranial laser PBM (810 nm wavelength and 100 mW power). We found that aging predominantly decreased regional brain CCO activity and systems-level functional connectivity, while the chronic laser stimulation predominantly reversed these age-related effects. We concluded that chronic PBM modified the effects of aging by causing the CCO activity on brain regions in laser-treated aged rats to reach levels similar to those found in young rats. Given the crucial role of CCO in bioenergetics, PBM may be used to augment brain and behavioral functions of older individuals by improving oxidative energy metabolism.