bluuebunny/crossref_metadata_2025 huggingface dataset:
Remodeling of Aortic Configuration and Abdominal Aortic Branch Perfusion After Endovascular Repair of Acute Type B Aortic Dissection: A Computed Tomographic Angiography Follow-Up
Authors: Yuan Zihui , Li Yiqing , Jin Bi , Wang Jian
Published: October 2021

Background: Thoracic endovascular aortic repair (TEVAR) for type B aortic dissection (TBAD) induces false lumen (FL) thrombosis, promotes favorable aortic remodeling, and makes an impact on abdominal aortic branch perfusion patterns. However, little is known about the long-term fate of aortic remodeling and abdominal aortic branch perfusion after TEVAR for TBAD and the effect of FL thrombosis status on these changes.

Materials and methods: Between January 2014 and May 2021, 59 enrolled patients with acute TBAD were treated with TEVAR and had post-operative or follow-up images. Pre-operative, post-operative, and latest follow-up CT angiography (CTA) data were analyzed for the largest diameter of true lumen (TL), FL, and transaorta and for the FL thrombosis status on the stented thoracic aorta, unstented thoracic aorta, and abdominal aorta. Abdominal aorta perfusion patterns were characterized.

Results: The mean follow-up period was 17.1 months. In the stented thoracic aorta, average TL diameters increased, average FL diameters decreased, and average transaortic diameters did not change; 82.6% of the patients had either a stable or shrinking transaortic size and 87% of the patients achieved total FL thrombosis. In the unstented thoracic aorta, average TL diameters increased, transaortic growth and no changes occurred in 39.1 and 45.7% of the patients, respectively, and complete FL thrombosis was present in 50% of the patients. In the abdominal aorta, average FL and transaortic diameters increased, aorta was expanded in 52.2% of the patients, and FL remained patent in 65.2% of the patients. Of the 354 branches, 37 branches (10.5%) exhibited changes in perfusion patterns, 22 branches (6.2%) demonstrated an increased TL perfusion, and 15 branches (4.2%) had an increased FL contribution. Compared with patent or partially thrombosed FL, complete FL thrombosis was accompanied by a bigger decrease in FL diameters, a larger increase in TL diameters, and a higher percentage of abdominal branch TL perfusion.

Conclusions: In majority of the patients, TEVAR stabilized the size of the stented thoracic aorta, namely TL expansion and FL obliteration. However, abdominal aortic FL remained patent FL, and it was expanded with the resultant transaortic growth over a long follow-up period. Abdominal aortic branch perfusion patterns remained largely stable after TEVAR. The failure to achieve FL thrombosis negatively affects the remodeling of a contagious abdominal aortic dissection.
Mesozoic Tectono-Thermal Event of the Qinshui Basin, Central North China Craton: Insights From Illite Crystallinity and Vitrinite Reflectance
Authors: Liu Runchuan , Ren Zhanli , Yang Peng , He Huaiyu , Smith Thomas M. , Guo Wei , Wu Lin
Published: October 2021

The Qinshui Basin is in the Central Orogenic Belt of the North China Craton (NCC), and the production of coalbed methane accounts for 70% of China’s total coalbed methane output. Aiming at the unclear dynamic causes of large-scale coalbed methane accumulation in the basin and the unclear response relationship with the destruction of the NCC, we present joint illite crystallinity and vitrinite reflectance study across the Zijin Mountain and Qincan 1 wells of the Qinshui Basin, respectively. Inverse modeling suggested that tectono-thermal events occurred during the Early Cretaceous, associated with the maximum burial depth and heat flow. The maximum paleo-geothermal temperature and gradient reconstruction results recorded at the Carboniferous strata are 180–190°C and 6.5°C/100 m. The denudation thicknesses recorded by illite crystallinity of Zijinshan and the vitrinite reflectance of the Qincan 1 Well are 3,180.63 m and 3,269.32 m, respectively. We propose that the Qinshui Basin was affected by the extensional environment of the NCC, which caused deep lithospheric thinning and magma upwelling, and a tectono-thermal event occurred during the Early Cretaceous in Qinshui Basin. In addition, the accumulation of coalbed methane, triggered by a tectono-thermal event during the Early Cretaceous, is consistent with the early Cretaceous accumulation and mineralization events in the NCC. Overall, our results reflect the subduction event influence of the western Pacific plate into the East Asian continental plate on the tectono-thermal history of the Central Orogenic Belt of the NCC, which is theoretically significant for clarifying the thermal lithospheric thickness and rheological structure of the basin, as well as the evolutionary history of coalbed methane, and the basin response relationship to the destruction of the NCC.
Examination of Longitudinal Seismic Vulnerability of Shield Tunnels Utilizing Incremental Dynamic Analysis
Authors: Dong Zhengfang , Kuo Chenyang , Yin Junhong , Wen Sen , Liu Ganzhi , Gou Yekai
Published: October 2021

In order to study the longitudinal seismic capacity of shield tunnels, this work applies the structural seismic vulnerability analysis, based on incremental dynamic analysis (IDA), to a shield tunnel and explores the ground motion intensity measure suitable for the shield tunnel in different site types. The failure probability of the structure at each earthquake intensity is calculated by combining the probabilistic seismic demand model with the limits on the engineering demand parameters to establish the seismic vulnerability curve of the structure. The results indicate that the peak ground velocity (PGV) is the ground motion intensity measure suitable for the longitudinal seismic performance of the shield tunnel. The site type has the most profound influence on the extent of the longitudinal damage to the shield tunnel, and site type IV is the most dangerous under an earthquake. Further, the tunnel has stronger seismic resistance in the elastoplastic stage. The low-grade bolts between the rings damage more severely than the high-grade bolts. A tunnel with either a great depth of burial or a large cross section is more dangerous than the one with either a small depth of burial or a small cross section. The risk of the axial tension-compression failure of the shield tunnel is higher than that of the horizontal transverse shear failure.
Comprehensive Evaluation of Water Carrying Capacity in Hebei Province, China on Principal Component Analysis
Authors: Wang Huadong , Xu Yunhong , Suryati Sulong Rini , Ma HuiLing , Wu Lifeng
Published: October 2021

Water is an important basic resource for social and economic development and also a necessity for the life and produce of people. The unbalanced development of water resources in Hebei Province of China and the obvious contradiction between supply and demand, affected by geography and natural environment change, has seriously influenced the Hebei village renewal process. This paper presents a comprehensive evaluation method of water carrying capacity—principal component analysis (PCA)—and constructs the evaluation index system of water carrying capacity in Hebei Province from water resources, water management, industrial development, agricultural development, social development, environmental protection, and other aspects. Based on the economic and water statistical data of Hebei province from 2009 to 2018, this paper adopts principal component analysis as an evaluation method to comprehensively evaluate the carrying capacity of water resources in Hebei Province across time and space. The results show that principal component analysis is an effective method for the comprehensive evaluation of water carrying capacity, which can reflect the local water carrying capacity objectively and comprehensively.
Environmental Drivers of Diversification and Hybridization in Neotropical Butterflies
Authors: Rueda-M Nicol , Salgado-Roa Fabian C. , Gantiva-Q Carlos H. , Pardo-Díaz Carolina , Salazar Camilo
Published: October 2021

Studying how the environment shapes current biodiversity patterns in species rich regions is a fundamental issue in biogeography, ecology, and conservation. However, in the Neotropics, the study of the forces driving species distribution and richness, is mostly based on vertebrates and plants. In this study, we used 54,392 georeferenced records for 46 species and 1,012 georeferenced records for 38 interspecific hybrids of the Neotropical Heliconius butterflies to investigate the role of the environment in shaping their distribution and richness, as well as their geographic patterns of phylogenetic diversity and phylogenetic endemism. We also evaluated whether niche similarity promotes hybridization in Heliconius. We found that these insects display five general distribution patterns mostly explained by precipitation and isothermality, and to a lesser extent, by altitude. Interestingly, altitude plays a major role as a predictor of species richness and phylogenetic diversity, while precipitation explains patterns of phylogenetic endemism. We did not find evidence supporting the role of the environment in facilitating hybridization because hybridizing species do not necessarily share the same climatic niche despite some of them having largely overlapping geographic distributions. Overall, we confirmed that, as in other organisms, high annual temperature, a constant supply of water, and spatio-topographic complexity are the main predictors of diversity in Heliconius. However, future studies at large scale need to investigate the effect of microclimate variables and ecological interactions.
Matching Data Types to the Objectives of Species Distribution Modeling: An Evaluation With Marine Fish Species
Authors: Luan Jing , Zhang Chongliang , Ji Yupeng , Xu Binduo , Xue Ying , Ren Yiping
Published: October 2021

Species distribution model (SDM) is a crucial tool for forecasting ranges of species and mirroring habitat references and quality. Different types of species distribution data have been commonly used in SDMs regarding different purposes and availability, whereas, the influences of data types on model performances have not been well understood. This study considered three data types characterized by different levels of organism information and cost in data acquisitions, namely presence/absence (P/A), ordinal data, and abundance data. We developed a range of distribution models for nine demersal species in the coastal waters of Shandong Peninsula, China, using two modeling algorithms [the Generalized Additive Model (GAM) and Random Forest]. Firstly, we evaluated the performances of all models on predicting species occurrence (i.e., habitat suitability or range boundaries), and then compared the models built with ordinal data and abundance data on projecting ordinal predictions (i.e., relative density or habitat quality). Their predictive abilities were assessed through cross-validation tests with diverse performance measurements. Overall, no data type is superior in all situations, but combined with two algorithms, the abundance data slightly outperformed the ordinal data and P/A data unexpectedly exerted reliable performances. Specifically, the effectiveness of data type for two application purposes of SDMs substantially varied with modeling algorithms, revealing that GAMs always benefit most from ordinal data and the opposite was true for Random Forest. For some small resident organisms with moderate prevalence, rough distribution data might be adopted for providing reliable projections. Our findings highlight the importance of clarifying the objectives of SDMs when choosing data types for species distribution modeling.
Transovarial Transmission of Dolichos Yellow Mosaic Virus by Its Vector, Bemisia tabaci Asia II 1
Authors: Ghosh Amalendu , Roy Buddhadeb , Nekkanti Aarthi , Das Amrita , Dhar Shri , Mukherjee Sunil Kumar
Published: October 2021

The cultivation of dolichos bean [Lablab purpureus (L.) Sweet] has been severely affected by dolichos yellow mosaic virus (DoYMV, Begomovirus) transmitted by whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). We tested the transovarial transmission of DoYMV in next-generation B. tabaci by PCR, real-time PCR, Southern blot hybridization, and biological transmission. The eggs, laid by DoYMV-exposed B. tabaci, carry the virus in a unique pattern. Only the eggs laid in between 3 and 6 days post virus acquisition by a parent B. tabaci were DoYMV positive. When tested individually in real-time PCR, around 31–53% of the eggs carried the virus. The presence of DoYMV in ovaries and F1 eggs was further substantiated by the hybridization of a Cy3-conjugated nucleic acid probe complementary to the viral strand of DoYMV. Viral DNA was also detected in F1 adults and F2 eggs. B. tabaci progenies carried not only the DoYMV DNA but were also infective. The F1 adults transmitted DoYMV to all tested plants and produced strong yellow mosaic symptoms. An increase in viral copies from egg to nymphal stage indicated propagation of DoYMV in B. tabaci. However, the increase was for a short period and decreased thereafter. The present study provides the first evidence of transovarial transmission and propagation of a bipartite begomovirus in its vector, B. tabaci Asia II 1. The transovarial transmission and replication of DoYMV in B. tabaci have great epidemiological relevance as B. tabaci can serve as a major host of the virus to bridge the gap between the cropping seasons.
Biofilm Formation Is Crucial for Efficient Copper Bioleaching From Bornite Under Mesophilic Conditions: Unveiling the Lifestyle and Catalytic Role of Sulfur-Oxidizing Bacteria
Authors: Bobadilla-Fazzini Roberto A. , Poblete-Castro Ignacio
Published: October 2021

Biofilm formation within the process of bioleaching of copper sulfides is a relevant aspect of iron- and sulfur-oxidizing acidophilic microorganisms as it represents their lifestyle in the actual heap/dump mining industry. Here, we used biofilm flow cell chambers to establish laminar regimes and compare them with turbulent conditions to evaluate biofilm formation and mineralogic dynamics through QEMSCAN and SEM-EDS during bioleaching of primary copper sulfide minerals at 30°C. We found that laminar regimes triggered the buildup of biofilm using Leptospirillum spp. and Acidithiobacillus thiooxidans (inoculation ratio 3:1) at a cell concentration of 106 cells/g mineral on bornite (Cu5FeS4) but not for chalcopyrite (CuFeS2). Conversely, biofilm did not occur on any of the tested minerals under turbulent conditions. Inoculating the bacterial community with ferric iron (Fe3+) under shaking conditions resulted in rapid copper recovery from bornite, leaching 40% of the Cu content after 10 days of cultivation. The addition of ferrous iron (Fe2+) instead promoted Cu recovery of 30% at day 48, clearly delaying the leaching process. More efficiently, the biofilm-forming laminar regime almost doubled the leached copper amount (54%) after 32 days. In-depth inspection of the microbiologic dynamics showed that bacteria developing biofilm on the surface of bornite corresponded mainly to At. Thiooxidans, while Leptospirillum spp. were detected in planktonic form, highlighting the role of biofilm buildup as a means for the bioleaching of primary sulfides. We finally propose a mechanism for bornite bioleaching during biofilm formation where sulfur regeneration to sulfuric acid by the sulfur-oxidizing microorganisms is crucial to prevent iron precipitation for efficient copper recovery.
Intracranial Compliance Concepts and Assessment: A Scoping Review
Authors: Ocamoto Gabriela Nagai , Russo Thiago Luiz , Mendes Zambetta Rafaella , Frigieri Gustavo , Hayashi Cintya Yukie , Brasil Sérgio , Rabelo Nicollas Nunes , Spavieri Júnior Deusdedit Lineu
Published: October 2021

Background: Intracranial compliance (ICC) has been studied to complement the interpretation of intracranial pressure (ICP) in neurocritical care and help predict brain function deterioration. It has been reported that ICC is related to maintaining ICP stability despite changes in intracranial volume. However, this has not been properly translated to clinical practice. Therefore, the main objective of this scoping review was to map the key concepts of ICC in the literature. This review also aimed to characterize the relationship between ICC and ICP and systematically describe the outcomes used to assess ICC using both invasive and non-invasive measurement methods.

Methods: This review included the following: (1) population: animal and humans, (2) concept of compliance or its inverse “elastance,” and (3) context: neurocritical care. Therefore, literature searches without a time frame were conducted on several databases using a combination of keywords and descriptors.

Results and Discussion: 43,339 articles were identified, and 297 studies fulfilled the inclusion criteria after the selection process. One hundred and five studies defined ICC. The concept was organized into three main components: physiological definition, clinical interpretation, and localization of the phenomena. Most of the studies reported the concept of compliance related to variations in volume and pressure or its inverse (elastance), primarily in the intracranial compartment. In addition, terms like “accommodation,” “compensation,” “reserve capacity,” and “buffering ability” were used to describe the clinical interpretation. The second part of this review describes the techniques (invasive and non-invasive) and outcomes used to measure ICC. A total of 297 studies were included. The most common method used was invasive, representing 57–88% of the studies. The most commonly assessed variables were related to ICP, especially the absolute values or pulse amplitude. ICP waveforms should be better explored, along with the potential of non-invasive methods once the different aspects of ICC can be measured.

Conclusion: ICC monitoring could be considered a complementary resource for ICP monitoring and clinical examination. The combination and validation of invasive/non-invasive or non-invasive measurement methods are required.
Influence of the Number of Channels and Classification Algorithm on the Performance Robustness to Electrode Shift in Steady-State Visual Evoked Potential-Based Brain-Computer Interfaces
Authors: Kim Hodam , Im Chang-Hwan
Published: October 2021

There remains an active investigation on elevating the classification accuracy and information transfer rate of brain-computer interfaces based on steady-state visual evoked potential. However, it has often been ignored that the performance of steady-state visual evoked potential (SSVEP)-based brain-computer interfaces (BCIs) can be affected through the minor displacement of the electrodes from their optimal locations in practical applications because of the mislocation of electrodes and/or concurrent use of electroencephalography (EEG) devices with external devices, such as virtual reality headsets. In this study, we evaluated the performance robustness of SSVEP-based BCIs with respect to the changes in electrode locations for various channel configurations and classification algorithms. Our experiments involved 21 participants, where EEG signals were recorded from the scalp electrodes densely attached to the occipital area of the participants. The classification accuracies for all the possible cases of electrode location shifts for various channel configurations (1–3 channels) were calculated using five training-free SSVEP classification algorithms, i.e., the canonical correlation analysis (CCA), extended CCA, filter bank CCA, multivariate synchronization index (MSI), and extended MSI (EMSI). Then, the performances of the BCIs were evaluated using two measures, i.e., the average classification accuracy (ACA) across the electrode shifts and robustness to the electrode shift (RES). Our results showed that the ACA increased with an increase in the number of channels regardless of the algorithm. However, the RES was enhanced with an increase in the number of channels only when MSI and EMSI were employed. While both ACA and RES values for the five algorithms were similar under the single-channel condition, both ACA and RES values for MSI and EMSI were higher than those of the other algorithms under the multichannel (i.e., two or three electrodes) conditions. In addition, EMSI outperformed MSI when comparing the ACA and RES values under the multichannel conditions. In conclusion, our results suggested that the use of multichannel configuration and employment of EMSI could make the performance of SSVEP-based BCIs more robust to the electrode shift from the optimal locations.
Distinct Effects of Short Chain Fatty Acids on Host Energy Balance and Fuel Homeostasis With Focus on Route of Administration and Host Species
Authors: Kong Dehuang , Schipper Lidewij , van Dijk Gertjan
Published: October 2021

Accumulating evidence implicates gut-microbiota-derived metabolites as important regulators of host energy balance and fuel homeostasis, the underlying mechanisms are currently subject to intense research. In this review, the most important executors, short chain fatty acids, which both directly and indirectly fulfill the interactions between gut microbiota and host will be discussed. Distinct roles of individual short chain fatty acids and the different effects they exert on host metabolism have long been overlooked, which compromises the process of clarifying the sophisticated crosstalk between gut microbiota and its host. Moreover, recent findings suggest that exogenously administered short chain fatty acids affect host metabolism via different mechanisms depending on the routes they enter the host. Although these exogenous routes are often artificial, they may help to comprehend the roles of the short-chain-fatty-acid mechanisms and signaling sites, that would normally occur after intestinal absorption of short chain fatty acids. Cautions should be addressed of generalizing findings, since different results have appeared in different host species, which may imply a host species-specific response to short chain fatty acids.
The Prognostic Value of Bone Morphogenetic Proteins and Their Receptors in Lung Adenocarcinoma
Authors: Meng Wangyang , Xiao Han , Zhao Rong , Li Dong , Li Kuo , Meng Yunchong , Chen Jiaping , Wang Yangwei , Liao Yongde
Published: October 2021

Background

Bone morphogenetic proteins (BMPs) regulate tumor progression via binding to their receptors (BMPRs). However, the expression and clinical significance of BMPs/BMPRs in lung adenocarcinoma remain unclear due to a lack of systematic studies.

Methods

This study screened differentially expressed BMPs/BMPRs (deBMPs/BMPRs) in a training dataset combining TCGA-LUAD and GTEx-LUNG and verified them in four GEO datasets. Their prognostic value was evaluated via univariate and multivariate Cox regression analyses. LASSO was performed to construct an initial risk model. Subsequently, after weighted gene co-expression network analysis (WGCNA), differential expression analysis, and univariate Cox regression analysis, hub genes co-expressed with differentially expressed BMPs/BMPRs were filtered out to improve the risk model and explore potential mechanisms. The improved risk model was re-established via LASSO combining hub genes with differentially expressed BMPs/BMPRs as the core. In the testing cohort including 93 lung adenocarcinoma patients, immunohistochemistry (IHC) was performed to verify BMP5 protein expression and its association with prognosis.

Results

BMP2, BMP5, BMP6, GDF10, and ACVRL1 were verified as downregulated in lung adenocarcinoma. Survival analysis identified BMP5 as an independent protective prognostic factor. We also found that BMP5 was significantly correlated with EGFR expression and mutations, suggesting that BMP5 may play a role in targeted therapy. The initial risk model containing only BMP5 showed a significant correlation (HR: 1.71, 95% CI: 1.28−2.28, p: 3e-04) but low prognostic accuracy (AUC of 1-year survival: 0.6, 3-year survival: 0.6, 5-year survival: 0.63). Seventy-nine hub genes co-expressed with BMP5 were identified, and their functions were enriched in cell migration and tumor metastasis. The re-established risk model showed greater prognostic correlation (HR: 2.58, 95% CI: 1.92–3.46, p: 0) and value (AUC of 1-year survival: 0.72, 3-year survival: 0.69, and 5-year survival: 0.68). IHC results revealed that BMP5 protein was also downregulated in lung adenocarcinoma and higher expression was markedly associated with better prognosis (HR: 0.44, 95% CI: 0.23–0.85, p: 0.0145).

Conclusion

BMP5 is a potential crucial target for lung adenocarcinoma treatment based on significant differential expression and superior prognostic value.
Prevalence of the GFI1-36N SNP in Multiple Myeloma Patients and Its Impact on the Prognosis
Authors: Khandanpour Cyrus , Eisfeld Christine , Nimmagadda Subbaiah Chary , Raab Marc S. , Weinhold Niels , Seckinger Anja , Hose Dirk , Jauch Anna , Försti Asta , Hemminki Kari , Hielscher Thomas , Hummel Manuela , Lenz Georg , Goldschmidt Hartmut , Huhn Stefanie
Published: October 2021

Transcription factor Growth Factor Independence 1 (GFI1) regulates the expression of genes important for survival, proliferation and differentiation of hematopoietic cells. A single nucleotide polymorphism (SNP) variant of GFI1 (GFI1-36N: serine replaced by asparagine at position 36), has a prevalence of 5-7% among healthy Caucasians and 10-15% in patients with myelodysplastic syndrome (MDS) and acute myeloid leukaemia (AML) predisposing GFI-36N carriers to these diseases. Since GFI1 is implicated in B cell maturation and plasma cell (PC) development, we examined its prevalence in patients with multiple myeloma (MM), a haematological malignancy characterized by expansion of clonal PCs. Strikingly, as in MDS and AML, we found that the GFI1-36N had a higher prevalence among MM patients compared to the controls. In subgroup analyses, GFI1-36N correlates to a shorter overall survival of MM patients characterized by the presence of t(4;14) translocation and gain of 1q21 (≤3 copies). MM patients carrying gain of 1q21 (≥3 copies) demonstrated poor progression free survival. Furthermore, gene expression analysis implicated a role for GFI1-36N in epigenetic regulation and metabolism, potentially promoting the initiation and progression of MM.
Garments and Footwear for Chronic Pain
Authors: Tajerian Maral , Garcia Jaqueline
Published: October 2021

In most human societies, wearing clothing and shoes, particularly in public settings, is commonplace and may even be legally required. Consequently, there is an abundance of clothing and footwear options for individuals of different ages, genders, body shapes and catering to different needs such as workwear and active-wear. However, many of the available options may not be viable for the millions and pain sufferers worldwide, indicating a need for adaptive apparel for the pain patient. In this perspective manuscript, we focus on the availability and efficacy of clothing designed to prevent pain in the general population as well as reduce or treat pain in pain patients. Furthermore, we put forth some considerations for the construction of adaptive garments. Such efforts and needed and could significantly improve well-being and quality of life in the pain patient.
Lactobacillus rhamnosus GG for Cow's Milk Allergy in Children: A Systematic Review and Meta-Analysis
Authors: Tan Weifu , Zhou Zhicong , Li Wei , Lu Han , Qiu Zemin
Published: October 2021

Objective: Cow's milk allergy (CMA) is a common allergic disease. Probiotics have been suggested as a treatment for CMA, with Lactobacillus rhamnosus GG (LGG) being one of the important predominant choices. Despite reports on this topic, the effectiveness of application in CMA remains to be firmly established.

Methods: To assess the effects of LGG on CMA in children, the PubMed/Medline, Embase, Cochrane Library, and Web of Science databases were searched for studies on LGG in treatment of CMA, which were published in the English language.

Results: Ten studies were finally included. Significantly higher tolerability rates favoring LGG over controls were observed [risk ratio (RR), 2.22; 95% confidence interval (CI), 1.86–2.66; I2 = 0.00; moderate-quality evidence]. There were no significant differences in SCORAD values favoring LGG over the placebo (mean difference, 1.41; 95% CI, −4.99–7.82; p = 0.67; very low-quality evidence), and LGG may have improved fecal occult blood (risk ratio, 0.36; 95% CI, 0.14–0.92; p = 0.03; low-quality evidence).

Conclusion: We found that LGG may have moderate-quality evidence to promote oral tolerance in children with CMA and may facilitate recovery from intestinal symptoms. However, this finding must be treated with caution, and more gpowerful RCTs are needed to evaluate the most effective dose and treatment time for children with CMA.

Registration number: CRD42021237221.
Tradition to Pathogenesis: A Novel Hypothesis for Elucidating the Pathogenesis of Diseases Based on the Traditional Use of Medicinal Plants
Authors: Kuchta Kenny , Cameron Silke
Published: October 2021

Traditional medicines embody knowledge on medicinal plants that has been accumulated through cultural evolution over millennia. In the latter half of the 20th century, two approaches to medicinal plant research have been established: the “Bench to Bedside” and the “Bedside to Bench” approaches which serve primarily for the development of more efficient therapeutics. Here, we propose a third, novel approach: from “Tradition to Pathogenesis” which aims to understand the pathogenesis of diseases based on the cultural evolution of their respective empirical treatments. We analyse multiple examples of diseases where the acting mechanism of traditional treatments across multiple cultures points to the pathogenesis of the respective disease. E.g., many cultures traditionally treat rheumatism with anti-bacterial botanical drugs, which is at odds with our current understanding that rheumatism is an aseptic inflammation. Furthermore, gastric ailments have traditionally been treated with anti-infectious botanical drugs indicating local infections, as demonstrated by the discovery of Helicobacter pylori as a common cause of gastric ulcer. Understanding traditional treatments can thus help to elucidate the pathogenesis of the disease.
An Objective Approach to Identify Priority Rare Diseases for the Development of Solutions Reducing the Diagnostic Delay Based on French Data
Authors: Chazal Pierre Etienne , Aymé Ségolène
Published: October 2021

A timely diagnosis is a critical step to ensure a proper access to expert clinical management for patients. However, diagnosing rare diseases (RD) is a major challenge, as they are not only numerous but also extremely diverse in their expression and cause. This generates a long lag time between first symptoms and diagnosis, unanimously thought to be unacceptably long in many cases, and amenable to improvement. Digital technologies offer new opportunities for improving diagnosis and care in a sector with urgent needs. However, developing and testing digital solutions would only be possible for a limited number of rare diseases (RD). The approach presented here aims at proposing an objective way of defining a subset of “priority” RD to focus on for the development and test of new solutions to reduce the time to diagnosis. An approach which is relevant not only when developing and testing new digital solutions but also organizational solutions in the field of RDs. The priority RDs presented herein have been highlighted using two objective criteria: the existence of a well-defined and established standard of care management, defined as the availability of a medicinal product specifically targeting the disease; and / or the existence of authoritative clinical guidelines. Our approach, based on French data, led to the establishment of a list of 251 RD for which a delayed diagnosis would be especially detrimental for the patient. This work demonstrates the feasibility of identifying objectively a subset of RD at urgent needs for the development of solutions to reduce the delay to diagnosis, if choices have to be made, based on publicly and well-established available data. The proposed list needs to be updated and adapted to the local situation, and validated by experts to establish if the delay to diagnosis can be reduced.
Antiviral and Immunomodulatory Effects of Pelargonium sidoides DC. Root Extract EPs® 7630 in SARS-CoV-2-Infected Human Lung Cells
Authors: Papies Jan , Emanuel Jackson , Heinemann Nicolas , Kulić Žarko , Schroeder Simon , Tenner Beate , Lehner Martin D. , Seifert Georg , Müller Marcel A.
Published: October 2021

Treatment options for COVID-19 are currently limited. Drugs reducing both viral loads and SARS-CoV-2-induced inflammatory responses would be ideal candidates for COVID-19 therapeutics. Previous in vitro and clinical studies suggest that the proprietary Pelargonium sidoides DC. root extract EPs 7630 has antiviral and immunomodulatory properties, limiting symptom severity and disease duration of infections with several upper respiratory viruses. Here we assessed if EPs 7630 affects SARS-CoV-2 propagation and the innate immune response in the human lung cell line Calu-3. In direct comparison to other highly pathogenic CoV (SARS-CoV, MERS-CoV), SARS-CoV-2 growth was most efficiently inhibited at a non-toxic concentration with an IC50 of 1.61 μg/ml. Particularly, the cellular entry step of SARS-CoV-2 was significantly reduced by EPs 7630 pretreatment (10–100 μg/ml) as shown by spike protein-carrying pseudovirus particles and infectious SARS-CoV-2. Using sequential ultrafiltration, EPs 7630 was separated into fractions containing either prodelphinidins of different oligomerization degrees or small molecule constituents like benzopyranones and purine derivatives. Prodelphinidins with a low oligomerization degree and small molecule constituents were most efficient in inhibiting SARS-CoV-2 entry already at 10 μg/ml and had comparable effects on immune gene regulation as EPs 7630. Downregulation of multiple pro-inflammatory genes (CCL5, IL6, IL1B) was accompanied by upregulation of anti-inflammatory TNFAIP3 at 48 h post-infection. At high concentrations (100 μg/ml) moderately oligomerized prodelphinidins reduced SARS-CoV-2 propagation most efficiently and exhibited pronounced immune gene modulation. Assessment of cytokine secretion in EPs 7630-treated and SARS-CoV-2-coinfected Calu-3 cells showed that pro-inflammatory cytokines IL-1β and IL-6 were elevated whereas multiple other COVID-19-associated cytokines (IL-8, IL-13, TNF-α), chemokines (CXCL9, CXCL10), and growth factors (PDGF, VEGF-A, CD40L) were significantly reduced by EPs 7630. SARS-CoV-2 entry inhibition and the differential immunomodulatory functions of EPs 7630 against SARS-CoV-2 encourage further in vivo studies.
Primary Cilia Are Critical Regulators of White Adipose Tissue Expansion
Authors: Hilgendorf Keren I.
Published: October 2021

The primary cilium is a microtubule-based cellular protrusion found on most mammalian cell types in diverse tissues. It functions as a cellular antenna to sense and transduce a broad range of signals, including odorants, light, mechanical stimuli, and chemical ligands. This diversity in signals requires cilia to display a context and cell type-specific repertoire of receptors. Recently, primary cilia have emerged as critical regulators of metabolism. The importance of primary cilia in metabolic disease is highlighted by the clinical features of human genetic disorders with dysfunctional ciliary signaling, which include obesity and diabetes. This review summarizes the current literature on the role of primary cilia in metabolic disease, focusing on the importance of primary cilia in directing white adipose tissue expansion during obesity.
Identification of an Isoflavonoid Transporter Required for the Nodule Establishment of the Rhizobium-Fabaceae Symbiotic Interaction
Authors: Biała-Leonhard Wanda , Zanin Laura , Gottardi Stefano , de Brito Francisco Rita , Venuti Silvia , Valentinuzzi Fabio , Mimmo Tanja , Cesco Stefano , Bassin Barbara , Martinoia Enrico , Pinton Roberto , Jasiński Michał , Tomasi Nicola
Published: October 2021

Nitrogen (N) as well as Phosphorus (P) are key nutrients determining crop productivity. Legumes have developed strategies to overcome nutrient limitation by, for example, forming a symbiotic relationship with N-fixing rhizobia and the release of P-mobilizing exudates and are thus able to grow without supply of N or P fertilizers. The legume-rhizobial symbiosis starts with root release of isoflavonoids that act as signaling molecules perceived by compatible bacteria. Subsequently, bacteria release nod factors, which induce signaling cascades allowing the formation of functional N-fixing nodules. We report here the identification and functional characterization of a plasma membrane-localized MATE-type transporter (LaMATE2) involved in the release of genistein from white lupin roots. The LaMATE2 expression in the root is upregulated under N deficiency as well as low phosphate availability, two nutritional deficiencies that induce the release of this isoflavonoid. LaMATE2 silencing reduced genistein efflux and even more the formation of symbiotic nodules, supporting the crucial role of LaMATE2 in isoflavonoid release and nodulation. Furthermore, silencing of LaMATE2 limited the P-solubilization activity of lupin root exudates. Transport assays in yeast vesicles demonstrated that LaMATE2 acts as a proton-driven isoflavonoid transporter.
Mask Use Depends on the Individual, Situation, and Location—Even Without COVID-19 Transmission: An Observational Study in Shanghai
Authors: English Alexander S. , Li Xiaoyuan
Published: October 2021

COVID-19 has drastically altered people’s mask-wearing behaviors around the world. What is unknown is how long these mask behaviors will last post-COVID-19? To investigate how individual, situational, and locational factors influence mask use in the absence of community spread of COVID-19, we conducted an observational study in public areas in the megacity of Shanghai, China. Researchers coded people’s mask use in various suburban and urban districts and outdoor and indoor locations with and without mask requirements. Firstly, even without any local transmissions in more than 40days, 62% of the sample (N=1,282) still wore masks in public places. The data showed that people in more urban areas wore masks more often and that people wore masks in places where it was mandated. Women also wore masks more than men, and older people complied more with mask enforcement policies. We found that more densely populated districts and areas with more inflow of non-locals also predicted more mask use. We argue that the pandemic has long-lasting effects on human behavior like mask usage and reflects individuals’ continual conformity to new social norms.
Defining Optimal Post-prison Care for Those With Psychosis: A Delphi Study
Authors: Simpson Paul L. , Settumba Stella , Adily Armita , Ton Bianca , Butler Tony
Published: October 2021

Background: Early treatment (considered as early contact with community mental health services) and treatment retention are associated with reduced reoffending among those with a previous diagnosis of psychosis, yet the attributes of care required to best achieve this is largely unexplored for people with psychosis leaving prison. This study sought consensus from a sample of experts and consumers regarding the attributes of an “optimal model of care” for those with a prior episode of psychosis leaving prison in New South Wales, Australia.

Methods: A Delphi method was used, which involved establishing a consensus from a panel of 25 experts and consumers. Following three meetings, 34 model of care attributes and 168 attribute levels were generated for two rounds of online scoring. All attributes and levels were included in the final model if they scored “very important” or “extremely important;” or if the attribute was agreed on by 70% or more of participants. The participant retention rate across scoring rounds was 96% for Round 1 and 84% for Round 2, where consensus was reached. Two “member checking” procedures were undertaken to enhance the integrity of findings: a model “stress test” and an online consumer poll.

Results: Thirty-two attributes and 72 attribute levels were included in the final model across four components: pre-release care planning and coordination; treatments in community; diversion from prison; and evaluation. Member checking endorsed a person-centered approach with carers and peer-support central to care.

Conclusions: Participants agreed that an optimal model of care should involve a specialized team who works independent of community health service teams to directly deliver certain treatments and services while helping consumers to access external social an economic supports and services.
COVID-19 Risk Assessment for the Tokyo Olympic Games
Authors: Zhu Wenhui , Feng Jie , Li Cheng , Wang Huimin , Zhong Yang , Zhou Lijun , Zhang Xingyu , Zhang Tao
Published: October 2021

Introduction: As of June 7, 2021, the outbreak of Coronavirus Disease 2019 (COVID-19) has spread to more than 200 countries. The global number of reported cases is more than 172.9 million, with more than 3.7 million deaths, and the number of infected individuals is still growing rapidly. Consequently, events and activities around the world were canceled or postponed, and the preparation for sporting events were greatly challenged. Under such circumstances, about 11,000 athletes from ~206 countries are arriving in Tokyo for the 32nd Summer Olympic Games. Therefore, it is urgently necessary to assess the occurrence and spread risk of COVID-19 for the Games.

Objectives: To explore effective prevention and control measures for COVID-19 in large international events through simulations of different interventions according to risk assessment.

Methods: We used a random model to calculate the number of initial infected patients and used Poisson distribution to determine the number of initial infected patients based on the number of countries involved. Furthermore, to simulate the COVID-19 transmission, the susceptible-exposed-symptomatic-asymptomatic-recovered-hospitalized (SEIARH) model was established based on the susceptible-exposed-infectious-recovered (SEIR) mathematical model of epidemic diseases. According to risk assessment indicators produced by different scenarios of the simulated interventions, the risk of COVID-19 transmission in Tokyo Olympic Games was assessed.

Results: The current COVID-19 prevention measures proposed by the Japan Olympic Committee need to be enhanced. And large-scale vaccination will effectively control the spread of COVID-19. When the protective efficacy of vaccines is 78.1% or 89.8%, and if the vaccination rate of athletes reaches 80%, an epidemic prevention barrier can be established.
High Maintenance of Rhizosphere Soil C and N Equilibrium Regardless of Plant Species or Species Traits
Authors: Wakelin Steve A. , Matson A. , Wigley K. , Waller L. , Dickie I. A. , Whitehead David , Garrett L.
Published: October 2021

Factors affecting the deposition of carbon and nitrogen into the rhizosphere soil have important implications for natural and managed ecosystems. These include the invasiveness of plants, extent to which ecosystems sequester soil carbon, through to regulation of N flow within and from agricultural ecosystems. This study determined if the close elemental ratios often measured in soils are evident within the highly active rhizosphere compartment, or rather potentially emerge to a conserved ratio (over time) from different initial rhizosphere states. Toward this, we assessed the rhizosphere C and N content (and C:N ratio) of 37 plant species; these were further grouped into the categories provenance (native or exotic to New Zealand), form (forb, grass, shrub, or tree), root-based nitrogen fixation symbiosis (+/– N-fixation), or mycorrhization type. Furthermore, the potential nitrification rate (PNR) among the plant rhizosphere soils was quantified to explore relationships between nitrate formation and the total C and C:N ratio. Mycorrhization status, provenance, and form had no significant influence on nutrient status nor PNR in rhizosphere soil samples (p > 0.05). However, total C and total N were significantly increased in the rhizosphere of N-fixing species (p < 0.02). These increased in proportion, with the C:N remaining constant for both groups (~12.24; p = 0.79). Rhizosphere PNR did not vary with categories of plants tested and had no correlation to rhizosphere total C, total N, nor C:N ratio (p > 0.3 for all). Overall, this study showed that conservation of nutrient ratios often measured in soils are present within the rhizosphere, where initial inputs of C and N enter the soil ecosystems. With the exception of N-fixing plant species, rhizosphere soils retain remarkably high conservation in C, N, C:P, and PNR among key groupings.
The Additional Value of Endolymphatic Hydrops Imaging With Intratympanic Contrast for Diagnostic Work-Up—Experience From a Neurotology Center in Austria
Authors: Weitgasser Lennart , O‘Sullivan Anna , Schlattau Alexander , Roesch Sebastian
Published: October 2021

Objective: To illustrate the merit of hydrops imaging during clinical workup of dizziness and balance disorders.

Background: Ever since the first description of in-vivo endolymphatic hydrops imaging in 2007, this diagnostic tool has been implemented in an increasing number of centers. The more experience in its clinical application is gathered, the more it is possible to critically assess its potential value for the diagnostic workup. This article intends to provide information about the experience of handling and utilization of endolymphatic hydrops imaging in one of the first centers in Austria.

Methods: Retrospective analysis and review of clinical cases.

Results: Based on our experience of endolymphatic hydrops imaging (EHI), which was established in cooperation between our departments of radiology and otorhinolaryngology in 2017, we have exclusively used intratympanic application of a contrast agent prior to magnetic resonance imaging, as this approach provides high quality imaging results. In 42.6% of cases, EHI could lead to the diagnosis of MD or HED. Since precise vestibular examination is still necessary, EHI is not a tool to replace the clinical examination but rather to add significantly to the interpretation of the results.

Conclusion: Endolymphatic hydrops imaging represents a valuable, safe and well-applicable tool for evaluating cases with inconclusive clinical results. However, its potential additional diagnostic benefits rely on a correct indication based on prior thorough vestibular investigations.