bluuebunny/crossref_metadata_2025 huggingface dataset:
Ensuring safety and feasibility for resection of pediatric benign ovarian tumors by single-port robot-assisted laparoscopic surgery using the da Vinci Xi system
Authors: Xu Deqiang , Gao Heyun , Yu Shanzhen , Huang Guangbin , Lu Dan , Yang Kun , Zhang Wei , Zhang Wen
Published: August 2022

Background

Single-port robot-assisted laparoscopic surgery (S-RALS) is rarely applied in pediatric surgery. There is still no study on the application of S-RALS for resection of pediatric benign ovarian tumors. The current study aimed to investigate the safety and feasibility of S-RALS for resection of pediatric benign ovarian tumors using the da Vinci Xi system.

Methods

The clinical data of three patients who underwent S-RALS for resection of benign ovarian tumors in the Department of Pediatric Surgery, Zhongnan Hospital of Wuhan University from May 2020 to September 2021 were retrospectively analyzed. The mean age of these children was 7.9 years (5.8–9.3 years). One was a case of bilateral ovarian tumors, and the other two were cases of right ovarian tumors.

Results

All three patients successfully underwent the resection of ovarian tumors through S-RALS without conversion to laparotomy. The average operation time was 180 min (118–231 min). The average amount of blood loss was 20 ml (10–35 ml). No drainage tube was placed. All postoperative pathological types of ovarian tumors were mature cystic teratomas in the three cases. All patients started a liquid diet 2 h after surgery. The average length of postoperative hospital stay was 4.7 days (3–7 days). No tumor recurred, no surgical site hernia occurred, and the wound healed very well with a cosmetic scar in the lower umbilical crease during the postoperative follow-up for 6–18 months.

Conclusion

S-RALS has the advantages of less surgical trauma, quick postoperative recovery, and a cosmetic scar in the lower umbilical crease. It is safe, effective, and feasible for pediatric benign ovarian tumors.
Surgical techniques and indications for treatment of adult moyamoya disease
Authors: Nguyen Vincent N. , Parikh Kara A. , Motiwala Mustafa , Erin Miller L. , Barats Michael , Milton Camille , Khan Nickalus R.
Published: August 2022

Moyamoya disease (MMD) is a chronic, progressive cerebrovascular disease involving the occlusion or stenosis of the terminal portion of the internal carotid artery (ICA) and the proximal anterior and middle cerebral arteries. Adults with MMD have been shown to progressively accumulate neurological and cognitive deficits without treatment, with a mortality rate double that of pediatric patients with MMD. Surgical intervention is the mainstay of treatment to prevent disease progression and improve clinical outcomes. Several different types of bypasses can be utilized for revascularization in MMD, including indirect, direct, and combined forms of extracranial-to-intracranial (EC-IC) bypass. Overall, the choice of appropriate technique requires consideration of the age of the patient, preoperative hemodynamics, neurologic status, and territories most at risk and in need of revascularization. Here, we will review the indications and surgical techniques for the treatment of adult MMD. Step-by-step instructions for performing several bypass variants with technical pearls are discussed.
Prevalence of idiopathic epilepsy and structural epilepsy in 74 Boxer dogs in a referral hospital
Authors: Loncarica Tina , Balducci Federica , Bernardini Marco
Published: August 2022

The prevalence of idiopathic epilepsy and structural epilepsy in Boxer dogs is unknown. The aim of this retrospective study was to evaluate the prevalence of structural and idiopathic epilepsy in the Boxer population. A total of 74 Boxer dogs were included in the study from the database of one referral hospital and the following were recorded: signalment, history, clinical findings and results of advanced diagnostic imaging. Five dogs (6.8%) were diagnosed with idiopathic epilepsy, of which one was in the <6 months age group, three were in the 6–72 months age group and one was in the >72 months age group. Sixty-nine dogs (93.2%) were diagnosed with structural epilepsy. Sixty-six had a suspected intracranial neoplasia: Eight were in the 6–72 months age group and represent 66.7% of the dogs in that age group. The other fifty-eight were in the >72 months age group and represent 96.7% of the dogs in that age group. In our Boxer population, 81.8% of the patients had a suspected intra-axial tumor and 22.7% of dogs with an intracranial pathology nevertheless had a normal neurological examination. In conclusion, in the majority of boxer patients the cause of epilepsy is a suspected intracranial neoplasia regardless of the age at presentation. Considering the finding in this study of a low prevalence of presumed idiopathic epilepsy in the Boxer breed, it is recommended that patients who satisfy Tier I confidence level of the “International Veterinary Epilepsy Task Force” (IVETF) also undergo an MRI study of the brain.
Magnetic resonance imaging highlights the meningeal involvement in steroid responsive meningitis-arteritis and suggests the inflammation of the surrounding tissues (70 cases)
Authors: Remelli Carlotta , Martello Alba , Valentini Alessia , Contiero Barbara , Bernardini Marco
Published: August 2022

Introduction

Steroid-responsive meningitis-arteritis (SRMA) is an immune-mediated disorder of young dogs for which there is no definitive ante-mortem diagnostic test. Magnetic Resonance Imaging (MRI) can be used to explore other differentials and extensive reports about its usefulness in the diagnosis of SRMA are lacking. The aims of this study were to retrospectively investigate the characteristics of MRI studies of the cervical spine of dogs diagnosed with SRMA and to compare the diagnostic capability of MRI obtained with low-field and high-field units.

Materials and methods

This is a double center, retrospective case series. Databases were searched between 2008 and 2021 for dogs with a diagnosis of SRMA. Dogs were included if the following criteria were fulfilled: a diagnosis of cervical SRMA, results of CSF analysis, and MRI of the cervical spine available for re-evaluation.

Results

Seventy cases were selected. MRI abnormalities were found in 69 cases (98.6%). Enhancement of the meninges, nerve roots, synovium of the articular facets and paravertebral muscles was present in 61 (87.1%), 10 (14.3%), 34 (48.6%), and 34 (48.6%) cases, respectively, when considering all MRI. In the low-field MRI, enhancement of these structures was present in 45 (90%), 4 (8%), 21 (42%) and 23 (46%) cases, respectively. In the high-field MRI, enhancement of these structures was present in 16 (80%), 6 (30%), 13 (65%) and 11 (55%) cases, respectively. Fat suppressed T1W images showed meningeal enhancement better than T1W images. When all the MRIs were considered, a significant increase in cell count of the cerebrospinal fluid was found between the three groups based on the meningeal MRI score (p = 0.001). In cases with no meningeal enhancement but enhancement of synovium of the articular facets and/or muscles a significantly lower cerebrospinal fluid cell count was present (p = 0.043), when considering all MRIs.

Conclusions

The most frequent detection on cervical MRI of dogs affected by SRMA is meningeal enhancement, often accompanied by enhancement of the synovium of the articular facets and/or muscular enhancement. Both low-field and high-field MRI have good diagnostic capability but the latter enables a more thorough investigation thanks to specific sequences. MRI is useful as a complementary tool to cerebrospinal fluid analysis.
Genomic insight into the integrative conjugative elements from ICEHpa1 family
Authors: Sun Huarun , Zhang Junkai , Miao Qingqing , Zhai Yajun , Pan Yushan , Yuan Li , Yan Fengbin , Wu Hua , Hu Gongzheng
Published: August 2022

Integrative conjugative elements (ICEs) are important carriers for disseminating resistance genes. We have previously reported a novel element ICEHpa1 carrying seven antibiotic resistance genes, which could be self-transmissible relying on the novel T4SS. To identify novel ICEHpa1 variants from 211 strains and novel T4SS encoded in ICEHpa1, and to explore the relationships in these ICEs, four complete sequences of ICEs were identified by WGS analysis and antimicrobial susceptibility testing was determined by broth microdilution. In addition, a comparative analysis of these ICEs was conducted with bioinformatic tools, and the transfer abilities of these ICEs were confirmed by conjugation. Four ICEHpa1 variants ICEGpa1818, ICEGpa1808, ICEGpa1807, and ICEGpa1815 with different resistance gene profiles were characterized, and their hosts showed different resistance spectrums. All ICEs shared the same backbone and were inserted into the tRNALeu site, and all resistance regions were inserted into the same target site between the accessory and integration regions. This study analyzed complete sequences of ICEs from the ICEHpa1 family and identified novel T4SS and insertion element ISGpa2. Diverse resistance genes extensively exist in these ICEs, serving as a reservoir for resistance genes and facilitating their dissemination.
Nationwide Population-Based Study About Patterns of Prescription Opioid Use and Misuse Among Young Adults in Spain
Authors: Carrasco-Garrido Pilar , Gallardo-Pino Carmen , Jiménez-Trujillo Isabel , Hernández-Barrera Valentín , García-Gómez-Heras Soledad , Lima Florencio Lidiane , Palacios-Ceña Domingo
Published: August 2022

Objective: Prescription opioid misuse has become one of the most common ways drugs are consumed among young adults. The objective of our study was to describe the prevalence and factors associated with prescription opioid use and misuse among young adults living in Spain.

Methods: A nationwide, cross-sectional epidemiological study on the use and misuse of prescription opioids in Spanish Youngers. We used individualized secondary data provided by the Household Survey on Alcohol and Drugs in Spain 2017–2018.

Results: Prevalence of prescription opioid use among young adults was 4.89%. Misuse among this population reached prevalence values of 13.4%, with higher values observed among women . The variables associated with a greater probability of prescription opioid use and misuse were misuse of tranquilizers, sedatives, and sleeping pills, along with using cannabis and other illicit psychoactive drugs (aOR = 2.99; 95% CI: 1.10–8.15).

Conclusion: Prescription opioid use and misuse in Youngers has important implications for the Spanish public health system, because, even though not currently comparable to the situation in other countries, this drug use could be on the verge of creating similar problems.
GPNMB-Positive Cells in Head and Neck Squamous Cell Carcinoma—Their Roles in Cancer Stemness, Therapy Resistance, and Metastasis
Authors: Kawasaki Yohei , Suzuki Hitomi , Suzuki Shinsuke , Yamada Takechiyo , Suzuki Maya , Ito Ayumi , Hatakeyama Haruka , Miura Masahito , Omori Yasufumi
Published: August 2022

Objective: Despite the use of surgical and chemoradiation therapies, head and neck squamous cell carcinoma (HNSCC) still has a poor prognosis. Immune checkpoint inhibitors have been shown to prolong life expectancy but have limited efficacy. Glycoprotein nonmetastatic melanoma protein B (GPNMB) has received significant attention in breast cancer treatment, in which it has been associated with cancer stem cells (CSCs) and epithelial-mesenchymal transition (EMT); however, the function of GPNMB in HNSCC is completely unknown. This study aimed to clarify the characteristics of GPNMB-positive cells in vitro and their association with the prognosis by immunostaining clinical specimens.

Methods: We examined the sphere formation, invasion, and migration ability of GPNMB-positive cells in four HNSCC cell lines in vitro. We also immunostained biopsy specimens with GPNMB from 174 patients with HNSCC diagnosed, treated, and followed-up in our institution to evaluate overall survival and progression-free survival.

Results: GPNMB-positive cells showed enhanced sphere formation, invasion, and migration, suggesting that they could have CSC characteristics and the ability to induce EMT, as reported for breast cancer. Clinical specimens showed that overall survival was 39.4% and 57.8% (p = 0.045) and that progression-free survival was 27.6% and 51.6% (p = 0.013) for the high-expression and the low-expression groups, respectively, indicating poor prognosis for the high GPNMB group. The high GPNMB group was also more resistant to chemoradiation and bioradiotherapy. GPNMB was more highly expressed in metastatic lymph nodes than in the primary tumor.

Conclusion: GPNMB-positive cells might have CSC characteristics and induce EMT. Detailed functional analyses of GPNMB in HNSCC and the establishment of therapies targeting GPNMB will lead to improved prognoses.
Long-Term Effect of Tamarisk Plantation on Soil Physical Properties and Soil Salt Distribution in Coastal Saline Land
Authors: Li Jingsong , Yang Ce , Hussain Tabassum , Feng Xiaohui , Liu Xiaojing , Guo Kai
Published: August 2022

Ecological restoration of coastal land by planting salt-tolerant plants has been widely used to construct vegetation. The aim of this study was to evaluate the changes induced by tamarisk (Tamarix chinensis) shrub on coastal soil physical quality and as well as the corresponding impact on salt distribution in the soil. A field study was conducted on coastal saline land, North China, where tamarisk plantation was established 5-year-old (T-5yr) and 11-year-old (T-11yr), and compared with barren land as control (CK). Quantitative soil physical properties, soil physical quality index, soil salt distribution, and salt leaching were examined. The results indicated that planting tamarisk improved the coastal soil properties at higher degree in topsoil than in deep soil layers. Tamarisk plantation significantly increased soil organic carbon content and pH. It also enhanced the formation of soil large aggregates and porosity; however, reduction soil bulk density and salt content in topsoil were recorded. Soil physical quality index was positively correlated with root weight density of tamarisk, and soil of T-11 yr plantation exhibited the highest soil physical quality index, with promoted soil physical functions of supporting root growth and the resistance to soil degradation. In addition, tamarisk induced soil physical changes which enhanced the salt-leaching in rainy season and contributed to the homogeneous salt distribution in soil profile. Consequently, the ecological benefits of tamarisk vegetation turned coastal saline land into a fertile land by plant–soil interaction and the soil structure improvement, therefore, it prevented the natural soil accumulation by accelerating the salt leaching after tamarisk was restored. This study provides some insights into the mechanism of tamarisk on coastal soil restoration and its regulation of soil salt distribution.
Rumen Fermentation and Microbiome Responses to Enzymatic Hydrolysate of Cottonseed Protein Supplementation in Continuous In Vitro Culture
Authors: Zhou Jia , Ding Ziyue , Pu Qijian , Xue Benchu , Yue Shuangming , Guan Shengtao , Wang Zhisheng , Wang Lizhi , Peng Quanhui , Xue Bai
Published: August 2022

This study aimed to evaluate the effect of enzymatic hydrolysate of cottonseed protein (ECP) on the kinetic of gas production, rumen fermentation characteristics, and microbial diversity in continuous in vitro culture with a single factorial design of supplementation with various concentrations of ECP or yeast culture. Treatments were control (without supplementation, CON), supplementation with 10 g/kg Diamond-V XP yeast culture of substrate (XP), and supplementation with 6, 12 and 18 g/kg ECP of substrate (ECP1, ECP2, ECP3), each incubated with 30 mL of buffered incubation fluids and 200 mg of fermentation substrate in graduated glass syringes fitted with plungers for 48 h. Compared with the CON treatment, supplementation of XP yeast culture increased the cumulative gas production at 12 and 24 h, the concentration of ammonia nitrogen (NH3-N) concentration at 24 and 36 h, the concentration of microbial protein (MCP) concentration at 24 and 48 h, the molar butyrate proportion at 12, 24, and 48 h, the molar valerate proportion at 48 h, and the ratio of non-glucogenic to glucogenic acids (p < 0.05). Compared with the CON treatment, the concentration of MCP and the molar propionate proportion at 12 h were higher in the ECP1 treatment (p < 0.05); the cumulative gas production at 2, 4, and 12 h, the concentration of NH3-N at 36 h and the molar valerate proportion at 48 h were higher in the ECP2 treatment (p < 0.05); the cumulative gas production at 2, 12, and 48 h, the concentration of NH3-N at 12 and 36 h, the concentration of MCP at 12, 36, and 48 h, the molar butyrate proportion at 12 and 48 h, and the molar valerate proportion at 48 h were higher in the ECP3 treatment (p < 0.05). Compared with the CON treatment, supplementation with XP yeast culture significantly altered the relative abundance of the phyla Firmicutes, Kiritimatiellaeota, and Proteobacteria, while supplementation with ECP had minimal effect on bacterial diversity. The prediction of bacterial functions showed that the main gene functions of rumen bacteria are associated with carbohydrate metabolism, amino acid metabolism, and membrane transport. The findings of this study suggest that ECP can be used as a superior feed ingredient for ruminants, the suitable level of ECP was 18 g/kg in vitro experiment.
Effect of Black Soldier Fly (Hermetia illucens L.) Fat on Health and Productivity Performance of Dairy Cows
Authors: Nekrasov Roman V. , Ivanov Gennady A. , Chabaev Magomed G. , Zelenchenkova Aloyna A. , Bogolyubova Nadezhda V. , Nikanova Daria A. , Sermyagin Alexander A. , Bibikov Semen O. , Shapovalov Sergey O.
Published: August 2022

Due to the intensive development of technologies for obtaining protein, energy feed and biologically active supplements from insects, the feasibility and effectiveness of introducing these products into the rations of farm animals require further study. This research aims to study the possibility and effects of feeding dairy cows fat from the larvae of the black soldier fly (BSFLF). The composition and properties of the BSFLF have been studied. The research of the fatty acid composition of BSFLF showed a high content of saturated fatty acids, including 58.9% lauric acid. The experiment was performed on black-and-white cows at the beginning of lactation (control, D0 (n = 12) vs. experimental D10 (n = 12) and D100 (n = 12) groups, 10 and 100 g/head/day BSFLF, respectively. There was no negative effect of BSFLF feeding on the process of feed digestion. The pH of the rumen content decreased (6.80 ± 0.07 & 6.85 ± 0.09 vs. 7.16 ± 0.06, p < 0.05), with an increase in the number of infusoria (0.27 ± 0.03&0.37 ± 0.09 vs. 0.18 ± 0.03 g/100 mL, p = 0.16); there was an increase in the concentration of VFA in the rumen content of animals of the experimental groups by 2.1 (p < 0.05) and 3.81 (p < 0.01) (8.66 ± 0.46 & 10.37 ± 0.42 vs. 6.56 ± 0.29) mmol/100 mL. The average daily milk yield of Group D10 cows over the experimental period (d17–d177) was slightly higher than the control (by 4.9%, p = 0.24 vs. Group D0). At the same time, Group D100 cows showed a significant increase in natural-fat milk compared to controls (by 8.0%, p < 0.05 vs. Group D0) over the same experiment period. Analysis of the fatty acid composition of the milk of the experimental animals showed some changes in the fatty acid composition of milk under the influence of BSFLF feeding; these changes were especially noticeable in Group D10. Thus, it was found that feeding dairy cows BSFLF at different dosages leads to better indicators of pre-gastric digestion and productivity.
Neuroprotective Role of α-Lipoic Acid in Iron-Overload-Mediated Toxicity and Inflammation in In Vitro and In Vivo Models
Authors: Carota Giuseppe , Distefano Alfio , Spampinato Mariarita , Giallongo Cesarina , Broggi Giuseppe , Longhitano Lucia , Palumbo Giuseppe A. , Parenti Rosalba , Caltabiano Rosario , Giallongo Sebastiano , Di Rosa Michelino , Polosa Riccardo , Bramanti Vincenzo , Vicario Nunzio , Li Volti Giovanni , Tibullo Daniele
Published: August 2022

Hemoglobin and iron overload is considered the major contributor to intracerebral hemorrhage (ICH)-induced brain injury. Accumulation of iron in the brain leads to microglia activation, inflammation and cell loss. Current available treatments for iron overload-mediated disorders are characterized by severe adverse effects, making such conditions an unmet clinical need. We assessed the potential of α-lipoic acid (ALA) as an iron chelator, antioxidant and anti-inflammatory agent in both in vitro and in vivo models of iron overload. ALA was found to revert iron-overload-induced toxicity in HMC3 microglia cell line, preventing cell apoptosis, reactive oxygen species generation and reducing glutathione depletion. Furthermore, ALA regulated gene expression of iron-related markers and inflammatory cytokines, such as IL-6, IL-1β and TNF. Iron toxicity also affects mitochondria fitness and biogenesis, impairments which were prevented by ALA pre-treatment in vitro. Immunocytochemistry assay showed that, although iron treatment caused inflammatory activation of microglia, ALA treatment resulted in increased ARG1 expression, suggesting it promoted an anti-inflammatory phenotype. We also assessed the effects of ALA in an in vivo zebrafish model of iron overload, showing that ALA treatment was able to reduce iron accumulation in the brain and reduced iron-mediated oxidative stress and inflammation. Our data support ALA as a novel approach for iron-overload-induced brain damage.
Analysis of Spatial and Temporal Variations of the Near-Surface Wind Regime and Their Influencing Factors in the Badain Jaran Desert, China
Authors: Hu Ziying , Wang Guangpeng , Liu Yong , Shi Peijun , Zhang Guoming , Liu Jifu , Gu Yu , Huang Xichen , Zhang Qingyan , Han Xu , Wang Xueling , Du Jiewen , Li Ruoxin , Liu Lianyou
Published: August 2022

Wind regime is one of the main natural factors controlling the evolution and distribution of aeolian sand landforms, and sand drift potential (DP) is usually used to study the capacity of aeolian sand transport. The Badain Jaran Desert (BJD) is located where polar cold air frequently enters China. Based on wind data of eight nearby meteorological stations, this research is intended to explore the temporal variation and spatial distribution features of wind speed and DP using linear regression and cumulative anomaly method, and reveal the relationship between atmospheric circulation and wind speed with correlation analysis. We found that the wind speed and frequency of sand-blowing wind in the BJD decreased significantly during 1971–2016, and the wind speed obviously mutated in 1987. The regional wind speed change was affected by the Asian polar vortex, the northern hemisphere polar vortex and the Tibet Plateau circulation. The wind rose of the annual sand-blowing wind in this region was the “acute bimodal” type. Most of the annual wind directions clustered into the W-NW, and the prevailing wind direction was WNW. During 1971–2016, the annual DP, the resultant drift potential (RDP) and the directional variability (PDP/DP) in the desert showed an obvious downtrend, with a “cliff-like” decline in the 1980s and relative stable fluctuation thereafter. The BJD was under a low-energy wind environment with the acute bimodal wind regime. Wind speed, sand-blowing wind frequency and DP were high in the northeast and low in the southwest.
CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells
Authors: Vanneste Domien , Staal Jens , Haegman Mira , Driege Yasmine , Carels Marieke , Van Nuffel Elien , De Bleser Pieter , Saeys Yvan , Beyaert Rudi , Afonina Inna S.
Published: August 2022

Prostate cancer (PCa) is one of the most common cancer types in men and represents an increasing global problem due to the modern Western lifestyle. The signalling adapter protein CARD14 is specifically expressed in epithelial cells, where it has been shown to mediate NF-κB signalling, but a role for CARD14 in carcinoma has not yet been described. By analysing existing cancer databases, we found that CARD14 overexpression strongly correlates with aggressive PCa in human patients. Moreover, we showed that CARD14 is overexpressed in the LNCaP PCa cell line and that knockdown of CARD14 severely reduces LNCaP cell survival. Similarly, knockdown of BCL10 and MALT1, which are known to form a signalling complex with CARD14, also induced LNCaP cell death. MALT1 is a paracaspase that mediates downstream signalling by acting as a scaffold, as well as a protease. Recent studies have already indicated a role for the scaffold function of MALT1 in PCa cell growth. Here, we also demonstrated constitutive MALT1 proteolytic activity in several PCa cell lines, leading to cleavage of A20 and CYLD. Inhibition of MALT1 protease activity did not affect PCa cell survival nor activation of NF-κB and JNK signalling, but reduced expression of cancer-associated genes, including the cytokine IL-6. Taken together, our results revealed a novel role for CARD14-induced signalling in regulating PCa cell survival and gene expression. The epithelial cell type-specific expression of CARD14 may offer novel opportunities for more specific therapeutic targeting approaches in PCa.
The Development of Human Navigation in Middle Childhood: A Narrative Review through Methods, Terminology, and Fundamental Stages
Authors: Pullano Luca , Foti Francesca
Published: August 2022

Spatial orientation and navigation are fundamental abilities in daily life that develop gradually during childhood, although their development is still not clear. The main aim of the present narrative review was to trace the development of navigational skills in middle childhood (6 to 12 years old) by means of studies present in the literature. To this aim, this review took into account the terminology, methodologies, different paradigms, and apparatuses used to investigate egocentric self-centered and allocentric world-centered representations, besides the different types of spaces (reaching/small/large; physical/virtual). Furthermore, this review provided a brief description of the development of navigational strategies and competences in toddlers and preschool children (0–5 years). The main result of this review showed how middle childhood is a crucial period for the improvement and development of allocentric strategies, including metric information. In fact, during this developmental window, children learn to handle proximal and distal cues, to transpose paper and virtual information into real environments, up to performing similarly to adults. This narrative review could represent a starting point to better clarify the development of navigation and spatial orientation, finalized to trace a development curve useful to map normal development and to have a term of comparison to assess performance in atypical development.
Insights into Circulating Tumor Cell Clusters: A Barometer for Treatment Effects and Prognosis for Prostate Cancer Patients
Authors: Lu Linyao , Hu Wei , Liu Bingli , Yang Tao
Published: August 2022

Prostate cancer (PCa) exhibits high cellular heterogeneity across patients. Therefore, there is an urgent need for more real-time and accurate detection methods, in both prognosis and treatment in clinical settings. Circulating tumor cell (CTC) clusters, a population of tumor cells and non-malignant cells in the blood of patients with tumors, are a promising non-invasive tool for screening PCa progression and identifying potential benefit groups. CTC clusters are associated with tumor metastasis and possess stem-like characteristics, which are likely attributable to epithelial–mesenchymal transition (EMT). Additionally, these biological properties of CTC clusters, particularly androgen receptor V7, have indicated the potential to reflect curative effects, guide treatment modalities, and predict prognosis in PCa patients. Here, we discuss the role of CTC clusters in the mechanisms underlying PCa metastasis and clinical applications, with the aim of informing more appropriate clinical decisions, and ultimately, improving the overall survival of PCa patients.
Functional Outcome in Spinal Meningioma Surgery and Use of Intraoperative Neurophysiological Monitoring
Authors: Jesse Christopher Marvin , Alvarez Abut Pablo , Wermelinger Jonathan , Raabe Andreas , Schär Ralph T. , Seidel Kathleen
Published: August 2022

Data on intraoperative neurophysiological monitoring (IOM) during spinal meningioma (SM) surgery are scarce. The aim of this study was to assess the role of IOM and its impact on post-operative functional outcome. Eighty-six consecutive surgically treated SM patients were included. We assessed pre and post-operative Modified McCormick Scale (mMCS), radiological and histopathological data and IOM findings. Degree of cord compression was associated with preoperative mMCS and existence of motor or sensory deficits (p < 0.001). IOM was used in 51 (59.3%) patients (IOM-group). Median pre and post-operative mMCS was II and I, respectively (p < 0.001). Fifty-seven (66.3%) patients showed an improvement of at least one grade in the mMCS one year after surgery. In the IOM group, only one patient had worsened neurological status, and this was correctly predicted by alterations in evoked potentials. Analysis of both groups found no significantly better neurological outcome in the IOM group, but IOM led to changes in surgical strategy in complex cases. Resection of SM is safe and leads to improved neurological outcome in most cases. Both complication and tumor recurrence rates were low. We recommend the use of IOM in surgically challenging cases, such as completely ossified or large ventrolateral SM.
Updating Clear Cell Renal Cell Carcinoma (a Tribute to Prof. Ondrej Hes)
Authors: Manini Claudia , López José I.
Published: August 2022

This Special Issue provides an insight into critical issues concerning clear cell renal cell carcinomas (CCRCCs), reflecting the recent level of intricacy reached by renal oncology [...]
Immunotherapy of Neuroendocrine Neoplasms: Any Role for the Chimeric Antigen Receptor T Cells?
Authors: Fanciulli Giuseppe , Modica Roberta , La Salvia Anna , Campolo Federica , Florio Tullio , Mikovic Nevena , Plebani Alice , Di Vito Valentina , Colao Annamaria , Faggiano Antongiulio
Published: August 2022

Neuroendocrine neoplasms (NENs) are a heterogeneous group of tumors with variable clinical presentation and prognosis. Surgery, when feasible, is the most effective and often curative treatment. However, NENs are frequently locally advanced or already metastatic at diagnosis. Consequently, additional local or systemic therapeutic approaches are required. Immunotherapy, based on chimeric antigen receptor T cells (CAR-T), is showing impressive results in several cancer treatments. The aim of this narrative review is to analyze the available data about the use of CAR-T in NENs, including studies in both preclinical and clinical settings. We performed an extensive search for relevant data sources, comprising full-published articles, abstracts from international meetings, and worldwide registered clinical trials. Preclinical studies performed on both cell lines and animal models indicate a significant therapeutic effect of CAR-T cells in NENs. Ongoing and future clinical trials will clarify the possible role of these drugs in patients with highly aggressive NENs.
Meta-Analysis Reveals Both the Promises and the Challenges of Clinical Metabolomics
Authors: Roth Heidi E. , Powers Robert
Published: August 2022

Clinical metabolomics is a rapidly expanding field focused on identifying molecular biomarkers to aid in the efficient diagnosis and treatment of human diseases. Variations in study design, metabolomics methodologies, and investigator protocols raise serious concerns about the accuracy and reproducibility of these potential biomarkers. The explosive growth of the field has led to the recent availability of numerous replicate clinical studies, which permits an evaluation of the consistency of biomarkers identified across multiple metabolomics projects. Pancreatic ductal adenocarcinoma (PDAC) is the third-leading cause of cancer-related death and has the lowest five-year survival rate primarily due to the lack of an early diagnosis and the limited treatment options. Accordingly, PDAC has been a popular target of clinical metabolomics studies. We compiled 24 PDAC metabolomics studies from the scientific literature for a detailed meta-analysis. A consistent identification across these multiple studies allowed for the validation of potential clinical biomarkers of PDAC while also highlighting variations in study protocols that may explain poor reproducibility. Our meta-analysis identified 10 metabolites that may serve as PDAC biomarkers and warrant further investigation. However, 87% of the 655 metabolites identified as potential biomarkers were identified in single studies. Differences in cohort size and demographics, p-value choice, fold-change significance, sample type, handling and storage, data collection, and analysis were all factors that likely contributed to this apparently large false positive rate. Our meta-analysis demonstrated the need for consistent experimental design and normalized practices to accurately leverage clinical metabolomics data for reliable and reproducible biomarker discovery.
Management of Recurrent Meningiomas: State of the Art and Perspectives
Authors: Corniola Marco Vincenzo , Meling Torstein R.
Published: August 2022

Background: While meningiomas often recur over time, the natural history of repeated recurrences and their management are not well described. Should recurrence occur, repeat surgery and/or use of adjuvant therapeutic options may be necessary. Here, we summarize current practice when it comes to meningioma recurrence after initial surgical management. Methods: A total of N = 89 articles were screened. N = 41 articles met the inclusion criteria and N = 16 articles failed to assess management of meningioma recurrence. Finally, N = 24 articles were included in our review. Results: The articles were distributed as follows: studies on chemotherapy (N = 14), radiotherapy, protontherapy, and stereotaxic radiosurgery (N = 6), boron-neutron capture therapy (N = 2) and surgery (N = 3). No study seems to provide serious alternatives to surgery in terms of progression-free and overall survival. Recurrence can occur long after the initial surgery and also affects WHO grade 1 meningiomas, even after initial gross total resection at first surgery, emphasizing the need for a long-term and comprehensive follow-up. Conclusions: Surgery still seems to be the state-of-the-art management when it comes to meningioma recurrence, since none of the non-surgical alternatives show promising results in terms of progression-free and overall survival.
Identification of the Tumor Infiltrating Lymphocytes (TILs) Landscape in Pure Squamous Cell Carcinoma of the Bladder
Authors: Eismann Lennert , Rodler Severin , Buchner Alexander , Schulz Gerald Bastian , Volz Yannic , Bischoff Robert , Ebner Benedikt , Westhofen Thilo , Casuscelli Jozefina , Waidelich Raphaela , Stief Christian , Schlenker Boris , Ledderose Stephan
Published: August 2022

Background: Tumor infiltrating lymphocytes (TILs) are known as important prognostic biomarkers and build the fundament for immunotherapy. However, the presence of TILs and its impact on outcome in pure squamous cell carcinoma (SCC) of the bladder remains uncertain. Methods: Out of 1600 patients undergoing radical cystectomy, 61 patients revealed pure bladder SCC in the final histopathological specimen. Retrospectively, immunohistochemical staining was performed on a subset of TILs (CD3+, CD4+, CD8+, CD20+). Endpoints were overall survival (OS), cancer-specific survival (CSS) and progression-free survival (PFS). The Kaplan–Meier method was used to evaluate survival outcomes. Results: Strong infiltration of CD3+ was found in 27 (44%); of CD4+ in 28 (46%); of CD8+ in 26 (43%); and of CD20+ in 27 tumors (44%). Improved OS was observed for strong CD3+ (p < 0.001); CD4+ (p = 0.045); CD8+ (p = 0.001); and CD20+ infiltration (p < 0.001). Increased rates of PFS were observed for CD3+ (p = 0.025) and CD20+ TILs (p = 0.002). In multivariate analyses, strong CD3+ (HR: 0.163, CI: 0.044–0.614) and strong CD8+ TILs (HR: 0.265, CI: 0.081–0.864) were revealed as predictors for OS and the strong infiltration of CD20+ cells (HR: 0.095, CI: 0.019–0.464) for PFS. Conclusions: These first results of TILs in bladder SCC revealed predictive values of CD3+, CD8+ and CD20+.
Vitamin B12 Attenuates Changes in Phospholipid Levels Related to Oxidative Stress in SH-SY5Y Cells
Authors: Theiss Elena Leoni , Griebsch Lea Victoria , Lauer Anna Andrea , Janitschke Daniel , Erhardt Vincent Konrad Johannes , Haas Elodie Christiane , Kuppler Konstantin Nicolas , Radermacher Juliane , Walzer Oliver , Portius Dorothea , Grimm Heike Sabine , Hartmann Tobias , Grimm Marcus Otto Walter
Published: August 2022

Oxidative stress is closely linked to Alzheimer’s disease (AD), and is detected peripherally as well as in AD-vulnerable brain regions. Oxidative stress results from an imbalance between the generation and degradation of reactive oxidative species (ROS), leading to the oxidation of proteins, nucleic acids, and lipids. Extensive lipid changes have been found in post mortem AD brain tissue; these changes include the levels of total phospholipids, sphingomyelin, and ceramide, as well as plasmalogens, which are highly susceptible to oxidation because of their vinyl ether bond at the sn-1 position of the glycerol-backbone. Several lines of evidence indicate that a deficiency in the neurotropic vitamin B12 is linked with AD. In the present study, treatment of the neuroblastoma cell line SH-SY5Y with vitamin B12 resulted in elevated levels of phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and plasmalogens. Vitamin B12 also protected plasmalogens from hydrogen peroxide (H2O2)-induced oxidative stress due to an elevated expression of the ROS-degrading enzymes superoxide-dismutase (SOD) and catalase (CAT). Furthermore, vitamin B12 elevates plasmalogen synthesis by increasing the expression of alkylglycerone phosphate synthase (AGPS) and choline phosphotransferase 1 (CHPT1) in SH-SY5Y cells exposed to H2O2-induced oxidative stress.
External Electric Field Tailored Spatial Coherence of Random Lasing
Authors: Bian Yaoxing , Yuan Hongyu , Zhao Junying , Liu Dahe , Gong Wenping , Wang Zhaona
Published: August 2022

In this study, spatial coherence tunable random lasing is proposed by designing a random laser with separate coupling configuration between the gain medium and the scattering part. By using the polymer dispersion liquid crystal (PDLC) film with tunable scattering coefficient for supplying random scattering feedback and output modification, red, green and blue random lasers are obtained. By applying or removing electric field to manipulate the scattering intensity of the PDLC film, intensity and spatial coherence of these random lasing are then switched between the high or low state. This work demonstrates that controlling the external scattering intensity is an effective method to manipulate the spatial coherence of random lasing.
Synthesis of One-Dimensional Titanium Oxide Nanowires for Polyvinylidene Fluoride Membrane Optimization
Authors: Dilbraiz Muhammad Arsalan , Nawaz Mohsan , Imtiaz Mr. , Ahmad Pervaiz , Haq Sirajul , Rehman Zia Ur , Ullah Hameed , Khandaker Mayeen Uddin , Tamam Nissren , Sulieman Abdelmoneim , Bajaber Majed A.
Published: August 2022

Due to their beneficial characteristics, 1D nanowires have made significant advancements in different realms due to their large surface area, unique surface chemistry, and tunable transport properties. Herein, a comprehensive study of titanium oxide nanowires from titanium (IV) isopropoxide, with the addition of CNTs in the polymeric membrane, has been conducted to form crystals. This method is quite proficient for nanowire synthetization, incorporated with CNTs and polymeric membranes. Carbon nanotubes (CNTs) were used to enhance their properties with polymeric polyvinylidene fluoride PVDF membrane. The concentration ratio of titanium oxide in PVDF membrane was 1, 3, 5, 10, and 15%. The development of titanium oxide nanowires at the nano level shaped, as the emulsion electrospinning created 1D nanowires. The use of an additive makes the membrane more conductive. FT-IR, XRD, EDS, and SEM techniques were used for size, surface geometry, crystalline nature, blend membrane, and thin film composition determination. Thermal Gravimetric Analysis (TGA) analysis shows that weight loss with temperature increases at optimum level.
A Taxonomic Revision of Two Species Complexes Belonging to the Haplotricha Subsection in the Genus Onosma (Boraginaceae): A Realistic Approach to Plant Diversity
Authors: Nasab Farzaneh Khajoei , Mehrabian Ahmad Reza
Published: August 2022

The taxonomy of O. dichroantha, O. nervosa, and their allied species is extremely controversial among authors. The delimitation of these species was extremely confusing, and closely related species were separated by weak and non-diagnostic morphological traits. A taxonomic revision of these taxa is presented here based on our fieldwork and a thorough study of herbaria specimens. The species O. dichroantha and O. sharifii are regarded as synonyms of O. setosa, and O. maculata is reduced to synonymy with O. nervosa. In addition, the typification of accepted names and relevant synonyms, detailed morphological descriptions and brief comments on the relationships between species are provided. Besides, it is accentuated that the introduction of any new species should be based on complete evidence to avoid systematic complexity. The results of this study play an important role in solving the problems of plant diversity and in the conservation of plant diversity.
An Internet-Oriented Multilayer Network Model Characterization and Robustness Analysis Method
Authors: Zhang Yongheng , Lu Yuliang , Yang Guozheng , Hou Dongdong , Luo Zhihao
Published: August 2022

The Internet creates multidimensional and complex relationships in terms of the composition, application and mapping of social users. Most of the previous related research has focused on the single-layer topology of physical device networks but ignored the study of service access relationships and the social structure of users on the Internet. Here, we propose a composite framework to understand how the interaction between the physical devices network, business application network, and user role network affects the robustness of the entire Internet. In this paper, a multilayer network consisting of a physical device layer, business application layer and user role layer is constructed by collecting experimental network data. We characterize the disturbance process of the entire multilayer network when a physical entity device fails by designing nodal disturbance to investigate the interactions that exist between the different network layers. Meanwhile, we analyze the characteristics of the Internet-oriented multilayer network structure and propose a heuristic multilayer network topology generation algorithm based on the initial routing topology and networking pattern, which simulates the evolution process of multilayer network topology. To further analyze the robustness of this multilayer network model, we combined a total of six target node ranking indicators including random strategy, degree centrality, betweenness centrality, closeness centrality, clustering coefficient and network constraint coefficient, performed node deletion simulations in the experimental network, and analyzed the impact of component types and interactions on the robustness of the overall multilayer network based on the maximum component change in the network. These results provide new insights into the operational processes of the Internet from a multi-domain data fusion perspective, reflecting that the coupling relationships that exist between the different interaction layers are closely linked to the robustness of multilayer networks.
The Tourist and Recreational Potential of Cross-Border Regions of Russia and Kazakhstan during the COVID-19 Pandemic: Estimation of the Current State and Possible Risks
Authors: Tanina Anna , Tashenova Larissa , Konyshev Yevgeni , Mamrayeva Dinara , Rodionov Dmitriy
Published: August 2022

The development of tourism is associated with numerous risks that have a direct and indirect impact on the realization of tourist and recreational potential. In recent years, in addition to internal risks, the importance of external environmental risks (geopolitical and epidemiological) has increased. The COVID-19 pandemic is one of the foremost of these risks, and its effects on the development of regional tourism demands attention. The purpose of the study is to estimate the level of tourist and recreational potential of cross-border regions of the Russian Federation and Kazakhstan, and the possible risks during the COVID-19 pandemic. After the breakup of the USSR, one of the longest land borders in the world was established between Russia and Kazakhstan. The geographical scope of the study includes 12 constituent entities of the Russian Federation and 7 regions of Kazakhstan. Information posted on statistical portals, data from geographical atlases, and specialized websites of the executive authorities were used as the materials for the study. The tourist and recreational potential of the regions of the Russian Federation and Kazakhstan was estimated by the scorecard method, with the assignment of weight coefficients to indicators included in four main clusters: Natural Factors, Cultural and Historical Factors, Social and Economic Factors, and Infrastructure Support of Tourism. Additionally, the experience of studying risks associated with tourism development during the pandemic was summarized. The conclusions reached are indicative of different levels of tourism and recreational potential in cross-border regions of the Russian Federation and Kazakhstan, and the inconsistency of the industry’s structure. It was found that the COVID-19 pandemic had increased the number of risks for the realization of tourism and recreational potential, which must be taken into account when making management decisions. The authorities of cross-border regions can use the results of the research to adjust tourism policy under the current restrictions and increased global risks. The application of mechanisms and methods of territorial planning and management will depend on the level of tourism and recreational potential. For regions with high and above-average potential, the emphasis should be on participation in federal projects, the development of cluster initiatives, and the application of a diversification strategy. Regions with medium and low potential should focus on the domestic tourist flow, develop inter-regional cooperation, and focus on the strategy of gaining a competitive advantage.
Underground Storage of Green Hydrogen—Boundary Conditions for Compressor Systems
Authors: Bekebrok Heinz , Langnickel Hendrik , Pluta Adam , Zobel Marco , Dyck Alexander
Published: August 2022

The large-scale storage of hydrogen in salt caverns, modelled on today’s natural gas storage, is a promising approach to storing renewable energy over a large power range and for the required time period. An essential subsystem of the overall gas storage is the surface facility and, in particular, the compressor system. The future design of compressor systems for hydrogen storage strongly depends on the respective boundary conditions. Therefore, this work analyses the requirements of compressor systems for cavern storage facilities for the storage of green hydrogen, i.e., hydrogen produced from renewable energy sources, using the example of Lower Saxony in Germany. In this course, a hydrogen storage demand profile of one year is developed in hourly resolution from feed-in time series of renewable energy sources. The injection profile relevant for compressor operation is compared with current natural gas injection operation modes.