bluuebunny/crossref_metadata_2025 huggingface dataset:
Value of the loss of heterozygosity to BRCA1 variant classification
Authors: Santana dos Santos Elizabeth , Spurdle Amanda B. , Carraro Dirce M. , Briaux Adrien , Southey Melissa , Torrezan Giovana , Petitalot Ambre , Leman Raphael , Lafitte Philippe ,   kConFab Investigators, Meseure Didier , Driouch Keltouma , Side Lucy , Brewer Carole , Beck Sarah , Melville Athalie , Callaway Alison , Revillion Françoise , Folgueira Maria A. A. Koike , Parsons Michael T. , Thorne Heather , Vincent-Salomon Anne , Stoppa-Lyonnet Dominique , Bieche Ivan , Caputo Sandrine M. , Rouleau Etienne
Published: January 2022

At least 10% of the BRCA1/2 tests identify variants of uncertain significance (VUS) while the distinction between pathogenic variants (PV) and benign variants (BV) remains particularly challenging. As a typical tumor suppressor gene, the inactivation of the second wild-type (WT) BRCA1 allele is expected to trigger cancer initiation. Loss of heterozygosity (LOH) of the WT allele is the most frequent mechanism for the BRCA1 biallelic inactivation. To evaluate if LOH can be an effective predictor of BRCA1 variant pathogenicity, we carried out LOH analysis on DNA extracted from 90 breast and seven ovary tumors diagnosed in 27 benign and 55 pathogenic variant carriers. Further analyses were conducted in tumors with PVs yet without loss of the WT allele: BRCA1 promoter hypermethylation, next-generation sequencing (NGS) of BRCA1/2, and BRCAness score. Ninety-seven tumor samples were analyzed from 26 different BRCA1 variants. A relatively stable pattern of LOH (65.4%) of WT allele for PV tumors was observed, while the allelic balance (63%) or loss of variant allele (15%) was generally seen for carriers of BV. LOH data is a useful complementary argument for BRCA1 variant classification.
Automated grading of enlarged perivascular spaces in clinical imaging data of an acute stroke cohort using an interpretable, 3D deep learning framework
Authors: Williamson Brady J. , Khandwala Vivek , Wang David , Maloney Thomas , Sucharew Heidi , Horn Paul , Haverbusch Mary , Alwell Kathleen , Gangatirkar Shantala , Mahammedi Abdelkader , Wang Lily L. , Tomsick Thomas , Gaskill-Shipley Mary , Cornelius Rebecca , Khatri Pooja , Kissela Brett , Vagal Achala
Published: January 2022

Enlarged perivascular spaces (EPVS), specifically in stroke patients, has been shown to strongly correlate with other measures of small vessel disease and cognitive impairment at 1 year follow-up. Typical grading of EPVS is often challenging and time consuming and is usually based on a subjective visual rating scale. The purpose of the current study was to develop an interpretable, 3D neural network for grading enlarged perivascular spaces (EPVS) severity at the level of the basal ganglia using clinical-grade imaging in a heterogenous acute stroke cohort, in the context of total cerebral small vessel disease (CSVD) burden. T2-weighted images from a retrospective cohort of 262 acute stroke patients, collected in 2015 from 5 regional medical centers, were used for analyses. Patients were given a label of 0 for none-to-mild EPVS (< 10) and 1 for moderate-to-severe EPVS (≥ 10). A three-dimensional residual network of 152 layers (3D-ResNet-152) was created to predict EPVS severity and 3D gradient class activation mapping (3DGradCAM) was used for visual interpretation of results. Our model achieved an accuracy 0.897 and area-under-the-curve of 0.879 on a hold-out test set of 15% of the total cohort (n = 39). 3DGradCAM showed areas of focus that were in physiologically valid locations, including other prevalent areas for EPVS. These maps also suggested that distribution of class activation values is indicative of the confidence in the model’s decision. Potential clinical implications of our results include: (1) support for feasibility of automated of EPVS scoring using clinical-grade neuroimaging data, potentially alleviating rater subjectivity and improving confidence of visual rating scales, and (2) demonstration that explainable models are critical for clinical translation.
Biperiodic superlattices and transparent states in graphene
Authors: Alvarado-Goytia J. J. , Rodríguez-González R. , Martínez-Orozco J. C. , Rodríguez-Vargas I.
Published: January 2022

The transmission and transport properties of biperiodic graphene superlattices are studied theoretically. Special attention is paid to the so-called transparent states of biperiodic superlattices. A Dirac-like Hamiltonian is used to describe the charge carriers in graphene. The transfer matrix method and the Landauer–Büttiker formalism are implemented to obtain the transmittance and conductance, respectively. Similar results to those reported for Schrödinger electrons are obtained. However, in the case of Dirac electrons the splitted bands and the transparent states associated to the biperiodicity depend strongly on the angle of incidence as well as the character of the charge carriers. In fact, the dynamic of the splitted bands and transparent states is inverted for holes. The origin of transparent states is unveiled by obtaining an analytic expression for the transmittance. It is found that resonant transmission through single and double barriers gives rise to transparent states. Regarding the transport properties, it is possible to identify the fundamental changes caused by the biperiodicity. In particular, it is found a splitting, shifting, and diminishment of the conductance peaks with respect to the case of regular periodicity. This opens the door to corroborate experimentally the fundamental characteristics of biperiodic gated graphene superlattices through transport measurements.
Quorum sensing and iron-dependent coordinated control of autoinducer-2 production via small RNA RyhB in Vibrio vulnificus
Authors: Lee Keun-Woo , Wen Yancheng , Park Na-Young , Kim Kun-Soo
Published: January 2022

Roles for the non-coding small RNA RyhB in quorum-sensing and iron-dependent gene modulation in the human pathogen V. vulnificus were assessed in this study. Both the quorum sensing master regulator SmcR and the Fur-iron complex were observed to bind to the region upstream of the non-coding small RNA RyhB gene to repress expression, which suggests that RyhB is associated with both quorum-sensing and iron-dependent signaling in this pathogen. We found that expression of LuxS, which is responsible for the biosynthesis of autoinducer-2 (AI-2), was higher in wild type than in a ryhB-deletion isotype. RyhB binds directly to the 5′-UTR (untranslated region) of the luxS transcript to form a heteroduplex, which not only stabilizes luxS mRNA but also disrupts the secondary structure that normally obscures the translational start codon and thereby allows translation of LuxS to begin. The binding of RyhB to luxS mRNA requires the chaperone protein Hfq, which stabilizes RyhB. These results demonstrate that the small RNA RyhB is a key element associated with feedback control of AI-2 production, and that it inhibits quorum-sensing signaling in an iron-dependent manner. This study, taken together with previous studies, shows that iron availability and cell density signals are funneled to SmcR and RyhB, and that these regulators coordinate cognate signal pathways that result in the proper balance of protein expression in response to environmental conditions.
Long-term metal fume exposure assessment of workers in a shipbuilding factory
Authors: Wang Ying-Fang , Kuo Yu-Chieh , Wang Lin-Chi
Published: January 2022

This study aims to assess the metal fume exposure of welders and to determine exposure rates for similar exposure groups in a shipyard through the use of Near-field/Far-field (NF/FF) mathematical model and Bayesian decision analysis (BDA) technique. Emission rates of various metal fumes (i.e., total chromium (Cr), iron (Fe), lead (Pb), manganese (Mn), and nickel (Ni)) were experimentally determined for the gas metal arc welding and flux cored arc welding processes, which are commonly used in shipyards. Then the NF/FF field model which used the emission rates were further validated by welding simulation experiment, and together with long-term operation condition data obtained from the investigated shipyard, the predicted long-term exposure concentrations of workers was established and used as the prior distribution in the BDA. Along with the field monitoring metal fume concentrations which served as the likelihood distribution, the posterior decision distributions in the BDA were determined and used to assess workers’ long-term metal exposures. Results show that the predicted exposure concentrations (Cp) and the field worker’s exposure concentrations (Cm) were statistically correlated, and the high R2 (= 0.81–0.94) indicates that the proposed surrogate predicting method by the NF and FF model was adequate for predicting metal fume concentrations. The consistency in both prior and likelihood distributions suggests the resultant posterior would be more feasible to assess workers’ long-term exposures. Welders’ Fe, Mn and Pb exposures were found to exceed their corresponding action levels with a high probability (= 54%), indicating preventive measures should be taken immediately. The proposed approach provides a universal solution for conducting exposure assessment with usual limited number of personal exposure data.
Accuracy of vascular tortuosity measures using computational modelling
Authors: Kashyap Vishesh , Gharleghi Ramtin , Li Darson D. , McGrath-Cadell Lucy , Graham Robert M. , Ellis Chris , Webster Mark , Beier Susann
Published: January 2022

Severe coronary tortuosity has previously been linked to low shear stresses at the luminal surface, yet this relationship is not fully understood. Several previous studies considered different tortuosity metrics when exploring its impact of on the wall shear stress (WSS), which has likely contributed to the ambiguous findings in the literature. Here, we aim to analyze different tortuosity metrics to determine a benchmark for the highest correlating metric with low time-averaged WSS (TAWSS). Using Computed Tomography Coronary Angiogram (CTCA) data from 127 patients without coronary artery disease, we applied all previously used tortuosity metrics to the left main coronary artery bifurcation, and to its left anterior descending and left circumflex branches, before modelling their TAWSS using computational fluid dynamics (CFD). The tortuosity measures included tortuosity index, average absolute-curvature, root-mean-squared (RMS) curvature, and average squared-derivative-curvature. Each tortuosity measure was then correlated with the percentage of vessel area that showed a < 0.4 Pa TAWSS, a threshold associated with altered endothelial cell cytoarchitecture and potentially higher disease risk. Our results showed a stronger correlation between curvature-based versus non-curvature-based tortuosity measures and low TAWSS, with the average-absolute-curvature showing the highest coefficient of determination across all left main branches (p < 0.001), followed by the average-squared-derivative-curvature (p = 0.001), and RMS-curvature (p = 0.002). The tortuosity index, the most widely used measure in literature, showed no significant correlation to low TAWSS (p = 0.86). We thus recommend the use of average-absolute-curvature as a tortuosity measure for future studies.
MEG8 regulates Tissue Factor Pathway Inhibitor 2 (TFPI2) expression in the endothelium
Authors: Kremer Veerle , Bink Diewertje I. , Stanicek Laura , van Ingen Eva , Gimbel Theresa , Hilderink Sarah , Günther Stefan , Nossent Anne Yaël , Boon Reinier A.
Published: January 2022

A large portion of the genome is transcribed into non-coding RNA, which does not encode protein. Many long non-coding RNAs (lncRNAs) have been shown to be involved in important regulatory processes such as genomic imprinting and chromatin modification. The 14q32 locus contains many non-coding RNAs such as Maternally Expressed Gene 8 (MEG8). We observed an induction of this gene in ischemic heart disease. We investigated the role of MEG8 specifically in endothelial function as well as the underlying mechanism. We hypothesized that MEG8 plays an important role in cardiovascular disease via epigenetic regulation of gene expression. Experiments were performed in human umbilical vein endothelial cells (HUVECs). In vitro silencing of MEG8 resulted in impaired angiogenic sprouting. More specifically, total sprout length was reduced as was proliferation, while migration was unaffected. We performed RNA sequencing to assess changes in gene expression after loss of MEG8. The most profoundly regulated gene, Tissue Factor Pathway Inhibitor 2 (TFPI2), was fivefold increased following MEG8 silencing. TFPI2 has previously been described as an inhibitor of angiogenesis. Mechanistically, MEG8 silencing resulted in a reduction of the inhibitory histone modification H3K27me3 at the TFPI2 promoter. Interestingly, additional silencing of TFPI2 partially restored angiogenic sprouting capacity but did not affect proliferation of MEG8 silenced cells. In conclusion, silencing of MEG8 impairs endothelial function, suggesting a potential beneficial role in maintaining cell viability. Our study highlights the MEG8/TFPI2 axis as potential therapeutic approach to improve angiogenesis following ischemia.
Targeting of non-coding RNAs encoded by novel MYC enhancers inhibits the proliferation of human hepatic carcinoma cells in vitro
Authors: Choi Hae In , An Ga Yeong , Yoo Eunyoung , Baek Mina , Chai Jin Choul , Binas Bert , Lee Young Seek , Jung Kyoung Hwa , Chai Young Gyu
Published: January 2022

The proto-oncogene MYC is important for development and cell growth, however, its abnormal regulation causes cancer. Recent studies identified distinct enhancers of MYC in various cancers, but any MYC enhancer(s) in hepatocellular carcinoma (HCC) remain(s) elusive. By analyzing H3K27ac enrichment and enhancer RNA (eRNA) expression in cultured HCC cells, we identified six putative MYC enhancer regions. Amongst these, two highly active enhancers, located ~ 800 kb downstream of the MYC gene, were identified by qRT-PCR and reporter assays. We functionally confirmed these enhancers by demonstrating a significantly reduced MYC expression and cell proliferation upon CRISPR/Cas9-based deletion and/or antisense oligonucleotide (ASO)-mediated inhibition. In conclusion, we identified potential MYC enhancers of HCC and propose that the associated eRNAs may be suitable targets for HCC treatment.
Development of soil-less substrates capable of degrading organic nitrogen into nitrate as in natural soils
Authors: Meeboon Jamjan , Nishida Ryoya , Iwai Takashi , Fujiwara Kazuki , Takano Masao , Shinohara Makoto
Published: January 2022

Soil-less substrates are unable to catalyse nitrification because the addition of a high concentration of organic substances suppresses nitrification. We used a previously developed multiple parallel mineralization method, which enables the use of organic fertilizers in hydroponics, to support nitrification in soil-less substrates. In this method, microorganisms immobilized on porous substrates produced inorganic nitrate from organic substances, as in a natural soil. Phosphate and potassium ions were also released. Microorganisms produced nitrate from organic substances when immobilized on polyurethane resin, rockwool, vermiculite, oyster shell lime, and rice husk charcoal. The optimal amount of organic substance added daily to 100 mL of substrate held 6 mg of organic N. The optimal incubation temperature ranged from 25 to 42 °C. A high relative humidity (51% or higher) was more suitable than drier conditions (20%). The optimal amount of fish fertilizer added to the substrate was 6 mg organic N. The lower the C/N ratio of the organic substance, the better the result. Vegetable plants grew well on inoculated substrates but not on uninoculated substrates. These results show that soil-less substrates can be used to create artificial soils in which plants can be grown with the addition of organic fertilizer, as in a natural soil.
Neuronally-enriched exosomal microRNA-27b mediates acute effects of ibuprofen on reward-related brain activity in healthy adults: a randomized, placebo-controlled, double-blind trial
Authors: Burrows Kaiping , Figueroa-Hall Leandra K. , Kuplicki Rayus , Stewart Jennifer L. , Alarbi Ahlam M. , Ramesh Rajagopal , Savitz Jonathan B. , Teague T. Kent , Risbrough Victoria B. , Paulus Martin P.
Published: January 2022

This double-blind, randomized, within-subjects design evaluated whether acute administration of an anti-inflammatory drug modulates neuron-specific, inflammation-modulating microRNAs linked to macroscopic changes in reward processing. Twenty healthy subjects (10 females, 10 males) underwent a functional magnetic resonance imaging scan while performing a monetary incentive delay (MID) task and provided blood samples after administration of placebo, 200 mg, or 600 mg of ibuprofen. Neuronally-enriched exosomal microRNAs were extracted from serum and sequenced. Results showed that: (1) 600 mg of ibuprofen exhibited higher miR-27b-3p, miR-320b, miR-23b and miR-203a-3p expression than placebo; (2) higher mir-27b-3p was associated with lower insula activation during MID loss anticipation; and (3) there was an inverse relationship between miR-27b-3p and MID gain anticipation in bilateral putamen during placebo, a pattern attenuated by both 200 mg and 600 mg of ibuprofen. These findings are consistent with the hypothesis that miR-27b could be an important messaging molecule that is associated with regulating the processing of positive or negative valenced information.
On electrically tunable stacking domains and ferroelectricity in moiré superlattices
Authors: Bennett Daniel , Remez Benjamin
Published: January 2022

It is well known that stacking domains form in moiré superlattices due to the competition between the interlayer van der Waals forces and intralayer elastic forces, which can be recognized as polar domains due to the local spontaneous polarization in bilayers without centrosymmetry. We propose a theoretical model which captures the effect of an applied electric field on the domain structure. The coupling between the spontaneous polarization and field leads to uneven relaxation of the domains, and a net polarization in the superlattice at nonzero fields, which is sensitive to the moiré period. We show that the dielectric response to the field reduces the stacking energy and leads to softer domains in all bilayers. We then discuss the recent observations of ferroelectricity in the context of our model.
Discovery of signatures of fatal neonatal illness in vital signs using highly comparative time-series analysis
Authors: Niestroy Justin C. , Moorman J. Randall , Levinson Maxwell A. , Manir Sadnan Al , Clark Timothy W. , Fairchild Karen D. , Lake Douglas E.
Published: January 2022

To seek new signatures of illness in heart rate and oxygen saturation vital signs from Neonatal Intensive Care Unit (NICU) patients, we implemented highly comparative time-series analysis to discover features of all-cause mortality in the next 7 days. We collected 0.5 Hz heart rate and oxygen saturation vital signs of infants in the University of Virginia NICU from 2009 to 2019. We applied 4998 algorithmic operations from 11 mathematical families to random daily 10 min segments from 5957 NICU infants, 205 of whom died. We clustered the results and selected a representative from each, and examined multivariable logistic regression models. 3555 operations were usable; 20 cluster medoids held more than 81% of the information, and a multivariable model had AUC 0.83. New algorithms outperformed others: moving threshold, successive increases, surprise, and random walk. We computed provenance of the computations and constructed a software library with links to the data. We conclude that highly comparative time-series analysis revealed new vital sign measures to identify NICU patients at the highest risk of death in the next week.
Heterogeneous digital biomarker integration out-performs patient self-reports in predicting Parkinson’s disease
Authors: Deng Kaiwen , Li Yueming , Zhang Hanrui , Wang Jian , Albin Roger L. , Guan Yuanfang
Published: January 2022

Parkinson’s disease (PD) is one of the first diseases where digital biomarkers demonstrated excellent performance in differentiating disease from healthy individuals. However, no study has systematically compared and leveraged multiple types of digital biomarkers to predict PD. Particularly, machine learning works on the fine-motor skills of PD are limited. Here, we developed deep learning methods that achieved an AUC (Area Under the receiver operator characteristic Curve) of 0.933 in identifying PD patients on 6418 individuals using 75048 tapping accelerometer and position records. Performance of tapping is superior to gait/rest and voice-based models obtained from the same benchmark population. Assembling the three models achieved a higher AUC of 0.944. Notably, the models not only correlated strongly to, but also performed better than patient self-reported symptom scores in diagnosing PD. This study demonstrates the complementary predictive power of tapping, gait/rest and voice data and establishes integrative deep learning-based models for identifying PD.
A biodegradable amphiphilic poly(aminoester) dendrimer for safe and effective siRNA delivery
Authors: Ma Chi , Zhu Dandan , Lin Wenyi , Li Ying , Huang Yuanzheng , Zhu Huiling , Ye Mengyun , Wang Yang , Peng Ling , Liu Xiaoxuan
Published: January 2022

An amphiphilic poly(aminoester) dendrimer demonstrating excellent siRNA delivery performance with advantageous biodegradability and the integrated delivery features of both lipid and polymer vectors.
Shape-tunable polymeric Janus nanoparticles with hollow cavities derived from polymerization induced self-assembly based crosslinked vesicles
Authors: Li Shanshan , Liu Xiaobo , Zhang Hao , Mao Yuhua , Zhang Tangxin , Wang Jianli
Published: January 2022

Hollow polymeric Janus nanoparticles with tunable shapes are derived from PISA-induced crosslinked vesicles by seed polymerization.
Decomposition mechanism of α-alkoxyalkyl-hydroperoxides in the liquid phase: temperature dependent kinetics and theoretical calculations
Authors: Hu Mingxi , Chen Kunpeng , Qiu Junting , Lin Ying-Hsuan , Tonokura Kenichi , Enami Shinichi
Published: January 2022

The decomposition rates of organic hydroperoxides derived from ozonolysis of terpene-alcohol in the presence of short-chain alcohols in aqueous organic media is markedly increased as the temperature is increased.
Direct conversion of CO2 to solid carbon by Ga-based liquid metals
Authors: Zuraiqi Karma , Zavabeti Ali , Clarke-Hannaford Jonathan , Murdoch Billy James , Shah Kalpit , Spencer Michelle J. S. , McConville Chris F. , Daeneke Torben , Chiang Ken
Published: January 2022

We present a simple alternative pathway to transform carbon dioxide to perpetually stored solid carbon.
Abiotic reduction of 3-nitro-1,2,4-triazol-5-one (NTO) and other munitions constituents by wood-derived biochar through its rechargeable electron storage capacity
Authors: Xin Danhui , Girón Julián , Fuller Mark E. , Chiu Pei C.
Published: January 2022

Quantitative conversion of NTO to ATO by reduced wood biochar can occur repeatedly through recharging of the biochar's electron storage capacity.
Confined ultrasmall MOF nanoparticles anchored on a 3D-graphene network as efficient and broad pH-adaptive photo Fenton-like catalysts
Authors: Shi Heng , He Yi , Li Yubin , He Teng , Luo Pingya
Published: January 2022

Confined ultrasmall metal–organic frameworks (MOFs) for efficient persulfate-based Fenton-like processes.
Microfluidic spinning of fucoxanthin-loaded nanofibers for enhancing antioxidation and clarification of fruit juice
Authors: Li Jiaxuan , Li Yu , Zhang Xuedi , Miao Song , Tan Mingqian , Su Wentao
Published: January 2022

Microfluidic spinning strategies to prepare composite fucoxanthin-loaded nanofibers for juice clarification and browning control simultaneously.
Enhanced antibacterial function of a supramolecular artificial receptor-modified macrophage (SAR-Macrophage)
Authors: Cheng Qian , Xu Meng , Sun Chen , Yang Kuikun , Yang Zhiqing , Li Junyan , Zheng Jun , Zheng Ying , Wang Ruibing
Published: January 2022

SAR-Macrophage was developed to rapidly recognizeE. colithrough strong host–guest interactions, thus improving the internalization ofE. coli, which subsequently induced M1 polarization of macrophages to kill bacteria.
A DFT study of SenTenclusters
Authors: Sharma Tamanna , Sharma Raman , Kanhere D. G.
Published: January 2022

The structural, electronic and optical properties of small SeTe clusters reveal that they have bearing on the formation of glassy Se–Te systems.
A biocatalytic approach towards the preparation of natural deoxyanthraquinones and their impact on cellular viability
Authors: Rajput Anshul , De Arijit , Mondal Amit , Das Kiran , Maity Biswanath , Husain Syed Masood
Published: January 2022

Natural deoxyanthraquinones synthesized using a chemoenzymatic approach and tested for cell viability shows less toxicity compared to the respective anthraquiones.
Transition-metal doped titanium-oxo clusters with diverse structures and tunable photochemical properties
Authors: Zheng Dongchun , Zhao Xiao-Li , Yan Xueqi , Xuan Weimin , Zheng Qi , Wang Lianjun , Jiang Wan
Published: January 2022

Transition metal doping effectively tuned the photochemical properties of titanium-oxo clusters {Ti2Mn4}, {Ti8Co5} and {Ti12Cd5}.
Merging the chemistry of metal–organic and polyoxometalate clusters to form enhanced photocatalytic materials
Authors: Napal Jon , Artetxe Beñat , Beobide Garikoitz , Castillo Oscar , Luque Antonio , Pascual-Colino Jon , Pérez-Yáñez Sonia , Perfecto-Irigaray Maite
Published: January 2022

The synergistic combination of a zirconium metal–organic cluster and a Keggin type polyoxotungstate led to a chemically stable and photoactive porous material.
The precise anti-tumor effect of a metallopolysaccharide-based nanotheranostic: turning phototherapy into programmed chemotherapy
Authors: Zhu Chengyuan , Guo Zhengxi , Yang Aijia , Jiang Bang-Ping , Liang Hong , Shen Xing-Can
Published: January 2022

A smart metallopolysaccharide-based nanotheranostic is fabricated through one-step coordinated-driven nanoassembly of Cu2+ and NH2-modified hyaluronic acid for a precise temporospatial anti-tumor effect.
Acid-responsive aggregated SERS nanoparticles for improved tumor diagnosis
Authors: Niu Xiaoyan , Yu Licheng , Wang Xiaohui , Zhang Zhenjie , Li Xiaomin , Feng Xiaoyue , Wang Wei , Yuan Zhi
Published: January 2022

The preparation and aggregation process of Au@MCPF NPs.
Difluorocarbene-enabled access to 1,3-oxazin-6-ones from enamides
Authors: Wang Shuai , Li Xin , Jin Shengnan , Liu Kang , Dong Cong , Su Jianke , Song Qiuling
Published: January 2022

1,3-Oxazin-6-ones as important structural scaffolds widely exist in many bioactive or therapeutic agents.
Temperature non-uniformity detection on dPCR chips and temperature sensor calibration
Authors: Gaňová Martina , Wang Xinlu , Yan Zhiqiang , Zhang Haoqing , Lednický Tomáš , Korabečná Marie , Neužil Pavel
Published: January 2022

We present a method of non-contact localized temperature measurement for determination of the non-uniformity of temperature distribution over a dPCR chip mounted on two different thermal cycling configurations.
Reaction mechanism of the green synthesis of glutaric acid
Authors: Hu Jie , Cheng Chun-Sheng , Liu Xuan , Ming Xu , Wei Zhen-Yun , Li Quan-Guo
Published: January 2022

In this study, the reaction mechanism underlying the green synthesis of glutaric acid was studiedviajoint test technology.
Recovery of copper, zinc and lead from photovoltaic panel residue
Authors: Xanthopoulos Panagiotis , Bevandić Srećko , Spooren Jeroen , Binnemans Koen , Kukurugya Frantisek
Published: January 2022

Extraction of copper, lead and zinc from photovoltaic panel residue by microwave-assisted chloride leaching, followed by recovery of extracted metals from the pregnant leaching solution by cementation, ion flotation and precipitation.
Radical boron migration of allylboronic esters
Authors: Tao Xiangzhang , Ni Shengyang , Kong Lingyu , Wang Yi , Pan Yi
Published: January 2022

A photocatalyzed 1,3-boron shift of allylboronic esters is reported. The atom-switch acrobatics proceeds via cascade 1,2-boron migrations and Smiles type rearrangement to furnish a variety of terminally functionalized alkyl boronates.
Sensing and imaging of PPi in vivo using lanthanide-based second near-infrared luminescent probes
Authors: Su Yaoquan , Ye Lingyue , Gu Jiayu , Zhao Lingzhi , Zhou Yunyun , Peng Juanjuan
Published: January 2022

A second near-infrared luminescent probe for the detection and imaging of pyrophosphate in vivo was developed.
Différenciation et compétitivité : quelle performance pour les exploitations viticoles françaises sous signe de qualité ?
Authors: Gillot Mélisande , Blasquiet-Revol Hélène , Jeanneaux Philippe
Published: January 2022

Les politiques de labellisation (Appellation d’origine protégée [AOP], Agriculture biologique [AB]) sont censées accroître le revenu des producteurs. Mais le revenu des agriculteurs n’est pas automatiquement amélioré par l’adoption d’une stratégie de différenciation. Nous analysons les performances économiques et techniques des exploitations ayant cette stratégie en évaluant leur efficacité technique pure et prix à l’aide de la méthode de Data Envelopment Analysis en 2014 et 2015. Nous utilisons les données du Réseau d’information comptable agricole et montrons que les exploitations en appellation d’origine protégée ont des scores d’efficacité plus faibles que celles n’ayant pas adopté ce signe de qualité. L’efficacité est variable selon les bassins viticoles. Il ressort par ailleurs que les exploitations en agriculture biologique ont des efficacités inférieures aux exploitations conventionnelles sur la période étudiée.
Investigation of the behavior of a heating system coupled to parabolic trough solar collector system
Authors: Mellaikhafi Abdellah , Tilioua Amine , Hajjaj Mohamed , Bouaaddi Abella , Messaoudi Choukri
Published: January 2022

The objective of this work is to study the thermal behavior of a solar heating system using an active layer, integrated in a wall and supplied with hot water by a parabolic trough solar collector (PTC). The prototype includes a PTC collector with a surface area of 1.8m2, a tank and an active layer integrated in a wall. A complete simulation model using the TRNSYS 16 simulation software has been established. Simulation tests under the climatic conditions of the city of Errachidia located in southeastern Morocco show the temperature variations in the different components of the installation as well as the useful energy gained during the three coldest months (December, January and February). In addition, an evaluation study of the system's performance with effect of different values of the reflectivity of PTC collector mirror was carried out. The results obtained show that the PTC efficiency increased from 0.41 to 0.63 when the reflectivity of the collector mirror increased from 0.6 to 0.9 for the three heating months.
Priority-based parking system for university campus using IOT and CVRP
Authors: Marouane EL Midaoui , Qbadou Mohammed , Mansouri Khalifa
Published: January 2022

The significant increase of cars and the population growth due to urban area development are considered as a challenge for the smart city last years. Resulting to traffic congestion, especially in the parking lot. One of the city major impacted infrastructures is the university campus. A parking management system saves time and resolves the traffic congestion. However, the rapid growth inside the university is not taking into consideration by the current parking management system. Also, the universities and other institutions are a hierarchical organism and have a specific priority and roles to be respected for spaces which include parking lot. The current paper addresses the parking management system inside universities with respect of the requirements of institution and proposes architecture based on IoT system to insure the flowless of the traffic inside the university. Also, a hybrid VRP and MDVRP solution to provide a fair distribution of parking lots between different visitors while respecting the priorities.
Comparisons of Cocoa Pod Husks and other Biomass Wastes for Syngas Production
Authors: Adeyanju Anthony Ademola
Published: January 2022

In this study, the characteristics of sawdust, switch grass, cocoa pod husks, and bagasse were investigated and compared, as well as the effects of blended nickel and silicon dioxide catalysts on syngas production by gasification utilising a fluidised bed reactor. Because of their high volatile matter and cellulose content as well as low moisture content, sawdust, switch grass, cocoa pod husks, and bagasse were found to be appropriate for generating syngas. Experimental analysis was conducted on the sawdust, switch grass, cocoa pod husks and bagasse samples with and without catalyst. The grains in the samples were comparable in size, ranging from 2mm to 6mm. The catalysts were revealed to have a distinct influence on the gasification process. The efficiency of the processes improves with catalysts, while the generation of CO, CO2, HC, and NO decreases. Cocoa pod husks produced the highest HC value of 7928 ppm without catalysts while bagasse produced 1679 ppm that is the lowest HC value.
DFT calculations on conjugated organic molecules based on thienothiophene for electronic applications
Authors: Elaati Allah Ismail , Zgou Hsaine , Amkassou Abdelkrim , Lafridi Hind
Published: January 2022

We report theoretical studies on the optoelectronic structural properties of five thienothiophene (T) conjugated π conjugates. The geometries, the prediction of the optoelectronic structural properties of the five compounds are studied by calculations of functional density theory (DFT). The absorption properties (λmax, Etr, f) of molecules are gained by the (DFT) B3LYP / 6-31G (d) ZINDO method, so that the most occupied molecular orbitals (HOMO), the least molecular orbitals occupied (LUMO), the energy deficit being calculated using the factor Gaussian 09 and its GaussView 5.0.8 graphical interface.
Cosmology with the SZ spectrum: Measuring the Universe’s temperature with galaxy clusters
Authors: Luzzi Gemma , D’Angelo Emanuele , Bourdin Herve , De Luca Federico , Mazzotta Pasquale , Oppizzi Filippo , Polenta Gianluca
Published: January 2022

The hot gas in clusters of galaxies creates a distinctive spectral distortion in the cosmic microwave background (CMB) via the Sunyaev-Zel’dovich (SZ) effect. The spectral signature of the SZ can be used to measure the CMB temperature at cluster redshift (TCMB(z)) and to constrain the monopole of the y-type spectral distortion of the CMB spectrum. In this work, we start showing the measurements of TCMB(z) for a sample extracted from the Second Catalog of galaxy clusters produced by Planck (PSZ2) and containing 75 clusters selected from CHEX-MATE. Then we show the forecasts for future CMB experiments about the constraints on the monopole of the y-type spectral distortion of the CMB spectrum via the spectrum of the SZ effect.
Crab nebula at 260 GHz with the NIKA2 polarimeter: Implications for the polarization angle calibration of future CMB experiments
Authors: Ritacco A. , Adam R. , Ade P. , Ajeddig H. , André P. , Artis E. , Aumont J. , Aussel H. , Beelen A. , Benoît A. , Berta S. , Bing L. , Bourrion O. , Calvo M. , Catalano A. , De Petris M. , Désert F.-X. , Doyle S. , Driessen E. F. C. , Gomez A. , Goupy J. , Kéruzoré F. , Kramer C. , Ladjelate B. , Lagache G. , Leclercq S. , Lestrade J.-F. , Macías-Pérez J.-F. , Maury A. , Mauskopf P. , Mayet F. , Monfardini A. , Muñoz-Echeverría M. , Perotto L. , Pisano G. , Ponthieu N. , Revéret V. , Rigby A. J. , Romero C. , Roussel H. , Ruppin F. , Schuster K. , Shu S. , Sievers A. , Tucker C. , Zylka R.
Published: January 2022

The quest for primordial gravitational waves enclosed in the Cosmic Microwave Background (CMB) polarization B-modes signal motivates the development of a new generation of high sensitive experiments (e.g. CMBS4, LiteBIRD), thus allowing to probe the inflationary epoch in the early Universe. However, this will be only possible by ensuring a high control of the instrumental systematic effects and an accurate absolute calibration of the polarization angle.

The Crab nebula is known to be a polarization calibrator on the sky for CMB experiments. Already used for the Planck satellite it exhibits a high polarized signal at microwave wavelengths. In this work we present Crab polarization observations obtained, in the 260 GHz frequency band, with the NIKA2 instrument. Furthermore, we discuss the accuracy needed on such a measurement to improve the constraints on the absolute angle calibration for CMB experiments.
Green sustainable supply chain under cap and trade regulation involving government introspection
Authors: Paul Arpita , Giri Bibhas Chandra
Published: January 2022

This paper investigates Government intervention in a three-echelon supply chain comprising one manufacturer and one retailer. Government is the top level member trying to reduce environmental impacts based on the amount of carbon emission during the production process. Government controls the chain by collecting tax from the retailer which is indirectly paid by the customer and paying subsidy/imposing fine on the manufacturer. Government encourages manufacturer to reduce carbon emission by contributing some subsidy and also makes an effort to generate Government net revenue (GNR) by imposing tax. The GNR is generated by collecting tax from the retailer on the sold product and penalty from the manufacturer at the trading price for the extra amount of emissions. The retail price is decided based on the selling price, tax and greening level. We aim to determine optimal levels of pricing, greening and amount of tax to be levied. The models for both linear and iso-elastic demand patterns are developed. The aim of this piece research is two-fold: (i) review the existent literature on the relationship between environmental collaboration and sustainability performance and (ii) render a tenable prototype of supply chain to illuminate the relationship between sustainability and profitability. According to the aforesaid goals this paper has carried out a detailed empirical research by using advanced structural equation modelling approaches. The research findings will be particularly important for manufacturing companies struggling to find techniques to achieve sustainability performance. Also it will aid the supply chains in developing environmental collaboration with the Govt. in order to attain the targets of GSCM.
Kleine Ursache – große Wirkung: Spättyp-Hauttests mit Medikamenten als Auslöser von disseminierten Rezidivexanthemen
Authors: Bircher Andreas J. , Jamiolkowski Dagmar , Steveling-Klein Esther H. , Scherer Hofmeier Kathrin , Mateluna Carlos Morales
Published: January 2022

Es werden zwei Patienten vorgestellt, die wegen eines streuenden allergischen Kontakt- bzw. Arzneimittelexanthems auf Budesonid bzw. Antibiotika abgeklärt wurden. Bei beiden trat innerhalb einiger Stunden nach Anlegen eines Epikutantests mit Budesonid bzw. Intradermaltests mit Amoxicillin eine stark positive Hauttestreaktion sowie ein Exanthem auf. Beim ersten Patienten lag eine Sensibilisierung auf die kreuzreagierenden Moleküle Budesonid und Amcinonid, bei der zweiten Patientin eine ausgeprägte Überempfindlichkeit auf Aminopenicilline vor. Aufflammphänomene (Flare-ups) sind v. a. aus der Kontaktallergologie bekannt, können aber auch bei der Abklärung von allergischen Arzneimittelexanthemen auftreten. Systemische Reaktionen vom verzögerten Typ auf Spättyp-Hauttests mit Medikamenten sind relativ selten, können aber eine erhebliche Morbidität bewirken. Verschiedene Formen von Aufflammphänomenen in der Allergologie werden diskutiert.
Robotisch assistierte totale Gastrektomie mit D2-Lymphadenektomie und intrakorporaler Rekonstruktion
Authors: Hoeppner Jens
Published: January 2022

Die robotisch assistierte totale Gastrektomie beim Magenkarzinom ist eine anspruchsvolle Operation, die in Asien, aber zunehmend auch in Europa in spezialisierten Zentren
durchgeführt wird. Die minimalinvasive Resektion, aber vor allem die Lymphadenektomie und minimalinvasive intrakorporale Ösophagojejunostomie sind technisch anspruchsvolle
operative Schritte, deren Technik international in verschiedenen Variationen durchgeführt wird. In dem vorgestellten Video zeigen wir die robotisch assistierte minimalinvasive
Technik der totalen Gastrektomie mit D2-Lymphadenektomie und intrakorporaler Rekonstruktion mittels Seit-zu-Seit-Ösophagojejunostomie in linearer Staplertechnik. Die
DaVinci-Xi-assistierte Operationstechnik wird detailliert beschrieben und in dem zugehörigen Video demonstriert.
Robotic Surgery for Ulcerative Colitis
Authors: Anderson Marissa , Grucela Alexis
Published: January 2022

Subtotal colectomy (STC) or total proctocolectomy (TPC) and ileal pouch–anal anastomosis (IPAA) performed in two or three stages remain the procedure of choice for patients with ulcerative colitis (UC). Minimally invasive laparoscopic approaches for STC and IPAA have been established for over a decade, having been shown to reduce postoperative pain, length of stay, and improve fertility. However “straight-stick” laparoscopy has ergonomic and visual disadvantages in the pelvis, which may contribute to IPAA failure. The robotic platform was developed to overcome these limitations. Robotic STC is associated with lower conversion rates and earlier return of bowel function with acceptably longer operative time (mean, 28 minutes) than laparoscopic STC. The robotic approach has also been shown in case series to be safe in urgent settings. Robotic IPAA is associated with lower blood loss and length of stay than laparoscopic IPAA. Robotic TPC/IPAA has been shown in small case series to be safe and feasible despite longer operating times.
A Privacy-Preserving Distributed Analytics Platform for Health Care Data
Authors: Welten Sascha , Mou Yongli , Neumann Laurenz , Jaberansary Mehrshad , Yediel Ucer Yeliz , Kirsten Toralf , Decker Stefan , Beyan Oya
Published: January 2022

Background In recent years, data-driven medicine has gained increasing importance in terms of diagnosis, treatment, and research due to the exponential growth of health care data. However, data protection regulations prohibit data centralisation for analysis purposes because of potential privacy risks like the accidental disclosure of data to third parties. Therefore, alternative data usage policies, which comply with present privacy guidelines, are of particular interest.

Objective We aim to enable analyses on sensitive patient data by simultaneously complying with local data protection regulations using an approach called the Personal Health Train (PHT), which is a paradigm that utilises distributed analytics (DA) methods. The main principle of the PHT is that the analytical task is brought to the data provider and the data instances remain in their original location.

Methods In this work, we present our implementation of the PHT paradigm, which preserves the sovereignty and autonomy of the data providers and operates with a limited number of communication channels. We further conduct a DA use case on data stored in three different and distributed data providers.

Results We show that our infrastructure enables the training of data models based on distributed data sources.

Conclusion Our work presents the capabilities of DA infrastructures in the health care sector, which lower the regulatory obstacles of sharing patient data. We further demonstrate its ability to fuel medical science by making distributed data sets available for scientists or health care practitioners.
Acute Arteriobiliary Fistula Emergent Long after Biliary Drainage and Radioembolization
Authors: Dökdök Murat , Karaman Kutlay
Published: January 2022

Various causes of hemobilia have been described in the literature, majority being iatrogenic. Other different pathologies such as inflammation and tumors also have been described. A 55-year-old male, who had a history of obstructive jaundice caused by a 4-cm liver mass treated with external-internal biliary drainage and transarterial radioembolization 6 months back, presented with acute hemobilia. Computed tomography demonstrated widespread radionecrosis of the right lobe and extravasation of intravascular contrast from right hepatic artery into common bile duct. Verified on angiography, the arteriobiliary fistula was managed with coil embolization.
Novel Resection System for Direct Endoscopic Necrosectomy of Walled-Off Pancreas Necrosis: Have We Conquered the Final Frontier?
Authors: Rana Surinder Singh , Gupta Rajesh
Published: January 2022

Surgical necrosectomy has been the traditional management for pancreatic necrosis and is done using blunt dissection. However, lack of dedicated endoscopic accessories has been a major limitation in direct endoscopic necrosectomy (DEN). Standard endoscopic accessories cannot effectively remove large necrotic material. Also, diameter of instrument channel of the endoscope limits the ability to use large-diameter graspers that can remove large chunks of necrotic material. In this news, we discuss a recent study that has evaluated a new powered endoscopic debridement system for DEN.
Surgical Outcome of Brunner's Gland Hamartoma: A Single-Centre Experience
Authors: Dhali Arkadeep , Ray Sukanta , Mandal Tuhin S. , Das Somak , Ghosh Ranajoy , Khamrui Sujan , Dhali Gopal K. , Sarkar Avik
Published: January 2022

Introduction Brunner's gland hamartomas (BGH) are rare benign lesions with an incidence of <0.01%, accounting for 5 to 10% of all benign tumors of the duodenum. It requires expeditious management by a multidisciplinary team. The aim of the study is to report our experience with surgery for BGH.

Methodology Data of all patients who underwent surgical intervention for duodenal polypoidal mass between August 2007 and March 2020 were retrieved from our prospectively maintained gastrointestinal (GI) surgery database. All patients whose histopathology report of the resected specimen confirmed BGH (n = 9) were included in the present study. Other pathological diagnosis like duodenal lipoma (n = 2), ganglioneuroma (n = 1), adenoma (n = 10), and adenocarcinoma (n = 4) were excluded.

Results Nine patients had confirmatory histopathological diagnosis of BGH and met our inclusion criteria. Three (33.3%) of them were men with a median age of 45 (range: 24–61) years. The median interval between onset of symptoms and diagnosis of duodenal polyp was 14 (range: 4–180) days. Five patients (55.5%) presented with upper GI hemorrhage. Three (33.3%) patients presented with abdominal pain, and one (11.1%) patient presented with episodes of bilious vomiting. Diagnostic endoscopy could detect the lesion in all (100%) patients. Contrast-enhanced computed tomography detected duodenal polypoidal lesion in five (55.5%) patients. The mean size of tumor was 4.78 ± 1.36 cm. These lesions were symptomatic in all the patients and warranted intervention. In view of failed endoscopic intervention (n = 7, 77.7%), or extramural extension of the tumor (n = 2, 22.2%), surgical intervention was considered. Most commonly performed operation was duodenal polypectomy (n = 6, 66.6%). Three postoperative complications developed in two (22.2%) patients. There was no surgery-related mortality. After a median follow-up of 60 (12 -78) months, no patient developed GI bleed or intestinal obstruction.

Conclusion In this study, the clinical profile of BGH was explored from the surgeon's point of view. Although endoscopic management is the first-line treatment, surgery plays an important role, particularly, if this fails or is not feasible. In experienced hand, surgery can be performed with acceptable perioperative morbidity and mortality and long-term satisfactory outcomes.