bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Fuel Economy and Engine Performance through Gasoline Fuel Octane Rating
Authors: Rodríguez-Fernández José , Ramos Ángel , Barba Javier , Cárdenas Dolores , Delgado Jesús
Published: July 2020

The octane number is a measure of the resistance of gasoline fuels to auto-ignition. Therefore, high octane numbers reduce the engine knocking risk, leading to higher compression threshold and, consequently, higher engine efficiencies. This allows higher compression ratios to be considered during the engine design stage. Current spark-ignited (SI) engines use knock sensors to protect the engine from knocking, usually adapting the operation parameters (boost pressure, spark timing, lambda). Moreover, some engines can move the settings towards optimized parameters if knock is not detected, leading to higher performance and fuel economy. In this work, three gasolines with different octane ratings (95, 98 and 100 RON (research octane number)) were fueled in a high-performance vehicle. Tests were performed in a chassis dyno at controlled ambient conditions, including a driving sequence composed of full-load accelerations and two steady-state modes. Vehicle power significantly increased with the octane rating of the fuel, thus decreasing the time needed for acceleration. Moreover, the specific fuel consumption decreased as the octane rating increased, proving that the fuel can take an active part in reducing greenhouse gas emissions. The boost pressure, which increased with the octane number, was identified as the main factor, whereas the ignition advance was the second relevant factor.
Numerical Study on the Gravity Effect on Heat Transfer of Supercritical CO2 in a Vertical Tube
Authors: Zhu Xiaojing , Zhang Ruizeng , Yu Xiao , Cao Maoguo , Ren Yongxiang
Published: July 2020

The effects of gravity on the heat transfer performance of supercritical CO2 flowing within a vertical tube with a diameter of 4.75 mm are numerically studied in this paper. The main objectives are to comprehensively investigate the action of gravity and buoyancy on the supercritical heat transfer. An effective numerical method, which employs a modified Shear Stress Transfer k-ω model (SST k-ω), is applied at various gravity conditions. It is found that, for both upward and downward flows, the heat transfer of supercritical CO2 is improved with increased gravity magnitude. The effect of gravity on heat transfer are more pronounced under a low mass flux condition than that under a high mass flux condition and it is closely related to the variations of thermal properties. For the upward flow, the increased gravity magnitude accelerates the near wall fluid and creates a classic “M-shaped” radial velocity distribution. For the downward flow, the increased gravity magnitude decelerates the near wall fluid and creates a parabola-like radial velocity distribution. On one hand, the turbulent kinetic energies of both the upward and downward flows are enhanced as the gravity magnitude increases, which benefits heat transfer dominated by turbulent eddy diffusion. On the other hand, high-density fluid with high thermal conductivity occupies the near wall region as the gravity magnitude increases, which benefits heat transfer dominated by molecular diffusion. The results might provide some instructive advice to improve the design and operation safety of heat exchanger at various gravity conditions.
Frametown: Addressing Declining Volunteerism through Empowering Female Engagement
Authors: Dunlap Scotty
Published: July 2020

The goal of this research is to identify the drivers that have resulted in an exceptionally high level of female engagement in the Frametown Volunteer Fire Department (FVFD) in the rural community of Frametown, West Virginia. Females comprise only 11% of the volunteer fire service in the US, however, they comprise approximately 60% of the Frametown Volunteer Fire Department, including the Chief, President, and Secretary. Structured interviews were used to investigate factors that have led to recruitment and retention of female volunteers. Interviews were conducted with 24 of the department’s 29 members with representation from female (n = 14) and male members (n = 10). Male members were included in the study to gain meaningful information from both gender perspectives as their story-telling added context to the evolution and current organizational culture of the department. Themes from the interviews included organic growth of the department, a sense of service to the community, a mentoring family environment, a heightened level of compassion as a value women bring to the fire department, and gender inclusiveness within the department as key recruitment and retention factors. The results of this study may provide insight into how other volunteer fire departments can increase female volunteers, particularly in an age of declining volunteerism.
Perspective of Membrane Technology in Pomegranate Juice Processing: A Review
Authors: Conidi Carmela , Drioli Enrico , Cassano Alfredo
Published: July 2020

Pomegranate (Punica granatum L.) juice is well recognized for its high content of phytochemicals with proven health-promoting effects. Conventional processing techniques including clarification with fining agents, pasteurization and thermal concentration have significant influences on bioactive compounds and antioxidant activity of the juice. The growing consumers demand for high-quality pomegranate juice as well as the industrial interest for the production of functional foods, nutraceuticals, and cosmetics from its bioactive compounds have promoted the interest for minimal processing technologies. In this context, membrane-based operations represent an innovative approach to improve the overall quality of pomegranate juice production. This review focuses on the recent advances and developments related to the application of membrane technology in pomegranate juice processing. Conventional pressure-driven membrane operations and innovative membrane operations, such as osmotic distillation and pervaporation, are discussed in relation to their potential in juice clarification, fractionation, concentration and aroma recovery. Their implementation in integrated systems offer new opportunities to improve the healthiness and quality of the juice as well as to recover, purify and concentrate bioactive compounds for the formulation of functional ingredients.
High-Throughput Detection and Characterization of Antimicrobial Resistant Enterococcus sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations
Authors: Anders Jennifer , Bisha Bledar
Published: July 2020

Antimicrobial resistant enteric bacteria can easily contaminate the environment and other vehicles through the deposition of human and animal feces. In turn, humans can be exposed to these antimicrobial resistant (AMR) bacteria through contaminated food products and/or contaminated drinking water. As wildlife are firmly established as reservoirs of AMR bacteria and serve as potential vectors in the constant spread of AMR, limiting contact between wildlife and livestock and effective tracking of AMR bacteria can help minimize AMR dissemination to humans through contaminated food and water. Enterococcus spp., which are known opportunistic pathogens, constantly found in gastrointestinal tracts of mammalian and avian species, swiftly evolve and cultivate AMR genotypes and phenotypes, which they easily distribute to other bacteria, including several major bacterial pathogens. In this study, we evaluated the use of high throughput detection and characterization of enterococci from wildlife [European starlings (Sturnus vulgaris)] by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) following culture-based isolation. MALDI-TOF MS successfully identified 658 Enterococcus spp. isolates out of 718 presumptive isolates collected from gastrointestinal tracts of European starlings, which were captured near livestock operations in Colorado, Iowa, Kansas, Missouri, and Texas; antimicrobial susceptibility testing was then performed using 13 clinically significant antibiotics.
Fruit-Based Beverages Contain a Wide Range of Phytochemicals and Intervention Targets Should Account for the Individual Compounds Present and Their Availability
Authors: Bestwick Charles , Scobbie Lorraine , Milne Lesley , Duncan Gary , Cantlay Louise , Russell Wendy
Published: July 2020

Benefits from micronutrients within fruit juice and smoothies are well documented, but fewer studies research the role of phytochemicals. Well-controlled human studies are essential to evaluate their impact, particularly on glucose and lipid regulation but also gastrointestinal health. Planning these studies requires data on the potential molecular targets. Here we report a comprehensive metabolomic (LC-MS) analysis of the phytochemical composition of four commonly consumed beverages, including data on whether they are free to be absorbed early in the gastrointestinal tract or bound to other plant components. Smoothies contained a wide range of phenolics (free and bound), whereas the fruit juices contained higher amounts of fewer compounds. Orange juice was rich in bound hesperidin (1.97 ± 0.39 mg/100 mL) and hydroxycinnamic acids, likely to be delivered to the colon with the potential to have an impact on gut health. Apple juice contained free chlorogenic acid (3.11 ± 1.03 mg/100 mL), phloridzin (0.40 ± 0.03 mg/100 mL), catechin (0.090 ± 0.005 mg/100 mL), and epicatechin (0.38 ± 0.02 mg/100 mL), suggesting potential roles in glucose uptake reduction or positive effects on systemic blood flow. Redox screening established that differences in chemical composition impacted on bioactivity, highlighting the importance of availability from the matrix. This suggests that fruit-based beverage interventions should target specific mechanisms depending on the fruits from which they are comprised and in particular, the availability of the individual constituents.
The Effect of Ethylene on the Color Change and Resistance to Botrytis cinerea Infection in ‘Kyoho’ Grape Fruits
Authors: Dong Tianyu , Zheng Ting , Fu Weihong , Guan Lubin , Jia Haifeng , Fang Jinggui
Published: July 2020

The formation of grape quality and the mechanism of resistance against foreign pathogens affect the storage stability of fruits during post-harvest handling. Ethylene plays a crucial role in regulating the ripeness of fruits and can be used as an exogenous regulator to resist exogenous pathogens. In this study, we used different concentrations of ethephon for treatment of grape fruits before veraison, analyzed the anthocyanin content, soluble solids, titratable acid, and determined fruit firmness and cell wall metabolism-related enzymes during fruit development. Results showed that exogenous ethephon promoted the early coloration of grape fruits and increased the coloring-related genes myeloblastosis A1(MYBA1), myeloblastosis A2(MYBA2), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3’-hydroxylase gene (F3’H), flavonoid 3’, 5’hydroxylase (F3’5’H), 3-O-flavonoid glucosyltransferase (UFGT), and glutathione S-transferase (GST), softening related genes Polygalacturonase(PG), pectinate lyases(PL) and Pectin methylesterase( PME, as well as ethylene metabolism pathway-related genes 1-aminocyclopropane-1-carboxylic acid synthase 1(ACS1), 1-aminocyclopropane-1-carboxylic acid oxidase 2 (ACO2), ethylene receptor gene(ETR2), and ethylene-insensitive 3 (EIN3). Ethephon treatment also increased soluble solids and decreased titratable acid in grape fruit. Fruits pretreated with ethephon were inoculated with Botrytis cinerea, which led to resistance in grape fruit through activation of the antioxidant system. The expression levels of disease resistance-related genes including VvPAD4, VvPIP1, VvNAC26, VvDREB, VvAPX, Vvpgip, VvWRKY70, VvMYC2, VvNPR1 also increased in inoculated fruit with pathogen following ethephon pretreatment. Furthermore, we monitored ethylene response factor 1(ERF1) transcription factor, which could interact with protein EIN3 during ethylene signal transduction and mediate fruit resistance against B. cinerea infection. Meanwhile, overexpression of VvERF1 vectorin strawberry fruits reduced the susceptibility to B. cinerea infection. We suggest that ethylene can induce resistance in ripened fruits after B. cinerea infection and provide adequate postharvest care.
Randomized Clinical Trial: The Effect of Exercise of the Intrinsic Muscle on Foot Pronation
Authors: Pabón-Carrasco Manuel , Castro-Méndez Aurora , Vilar-Palomo Samuel , Jiménez-Cebrián Ana María , García-Paya Irene , Palomo-Toucedo Inmaculada C.
Published: July 2020

Background: There is little scientific evidence regarding the effectiveness of strengthening exercises on the foot’s intrinsic musculature in improving the lower limb on the statics and dynamics in healthy individuals. Method: To evaluate the effect on foot posture with regard to the reinforcement of the short foot exercise (SFE) compared to another without a recognized biomechanical action, which we called the “non-biomechanical function” (NBF) exercise. A randomized clinical trial was carried out with 85 asymptomatic participants with a bilateral Foot Posture Index (FPI) greater than 6 points. An experimental group (n = 42) did SFE training and a control group (n = 43) carried out NBF exercises. The foot posture was evaluated twice via the navicular drop (ND) test, and the FPI was assessed on the day of inclusion in the study (pre-intervention) and after four weeks of training (post-intervention). Results: Statistically significant values were not found in foot posture between the experimental and the control groups when comparing before and after the training. However, the foot posture was modified in both groups with respect to its initial state, and the ND value decreased. Conclusions: SFE could be considered a useful tool to deal with pathologies whose etiology includes excessive pronation of the foot.
Analysis of Motor Intervention Program on the Development of Gross Motor Skills in Preschoolers
Authors: Ruiz-Esteban Cecilia , Terry Andrés Jaime , Méndez Inmaculada , Morales Ángela
Published: July 2020

This study aimed to investigate the influence of a structured movement activity program on the motor development of children aged three to five years attending preschool. Participants were 136 preschool students with normative development at three to four years old who lived in the Region of Murcia (Spain). The McCarthy Children’s Psychomotricity and Aptitude Scales (MSCA) battery of psychomotor tests was used to evaluate the motor development profiles of preschoolers before and after the intervention. The sample was divided into two groups: an intervention group (28 students) and a comparison group (108 students). A structured 24 week physical education program was used in the intervention group. An experiential program based on free play was used in the comparison group during the same period. Preschoolers in both groups got a significant improvement in the contrast of pre-intervention with post-intervention in limb coordination. Statistically significant differences in the post-intervention measurements between the comparison group and the intervention group on arm and leg coordination were observed, whereby the intervention group presented higher arm coordination values (F1,134 = 14,389, p = 0.000, η2 = 0.097) and higher leg coordination values (F1,134 = 19,281, p = 0.000, η2 = 0.126) than the comparison group. It was pointed out that structured physical activity education is better educational methodology than free play to achieve adequate motor development in preschool children.
Impact of Residential Green Space on Sleep Quality and Sufficiency in Children and Adolescents Residing in Australia and Germany
Authors: Feng Xiaoqi , Flexeder Claudia , Markevych Iana , Standl Marie , Heinrich Joachim , Schikowski Tamara , Koletzko Sibylle , Herberth Gunda , Bauer Carl-Peter , von Berg Andrea , Berdel Dietrich , Astell-Burt Thomas
Published: July 2020

Increasing evidence suggests adults living in greener areas tend to have more favourable sleep-related outcomes, but children and adolescents are under-researched. We hypothesised that children and adolescents living in greener areas would have better quality and more sufficient levels of sleep on average, especially within the context of high traffic noise exposure. These hypotheses were tested using multilevel logistic regressions fitted on samples from the nationally representative Longitudinal Study of Australian Children (10–11 years old, n = 3469, and 14–15 years old, n = 2814) and the GINIplus and LISA cohorts (10 years old, n = 1461, and 15 years old, n = 4172) from the Munich, Wesel, and Leipzig areas of Germany. Questionnaire-based binary indicators of sleep sufficiency and sleep quality in each cohort were assessed with respect to objectively measured green space exposures adjusting for age, sex, and maternal education. Models were augmented with proxy measures of traffic noise and two-way interaction terms to test for effect modification. Cross-tabulations illustrated little convincing evidence of association between green space and insufficient sleep or poor sleep quality in either sample, except for insufficient sleep among 10 year old participants in Germany. These null findings were replicated in adjusted models. The proxy for traffic noise was associated with poor quality sleep in 15 year old participants in Germany, but no convincing evidence of modified association with green space was observed.
Food Safety Conditions in Home-Kitchens: A Cross-Sectional Study in the Federal District/Brazil
Authors: Farias Adenilma da Silva , Akutsu Rita de Cassia Coelho de Almeida , Botelho Raquel Braz Assunção , Coelho Araújo Wilma Maria , Silva Izabel Cristina , Sávio Karin Eleonora , Zandonadi Renata Puppin
Published: July 2020

This study aimed to analyze the food safety conditions in home kitchens from the Brazilian Federal District. A previously validated instrument composed of 77 items (in four blocks) was used to evaluate the safety conditions in home kitchens. A survey was carried out with on-site application with 226 home kitchens’ food handlers in the Federal District, Brazil to evaluate Brazilian home kitchens’ good practices. Of the home kitchen food handlers, most of them were female (64.6%), had completed undergraduate education (29.2%), and were 45–59 years old (23.5%). The visited households had an average of 3.38 ± 1.48 residents that ate some of their meals at home, and 40% declared the monthly family income to be between 5 and 15 Brazilian minimum wages (MW). Regarding the reliability of the instrument, from the KR-20 test, it was verified that the instrument presents good internal consistency (α = 0.758). According to the instrument classification, the home kitchens’ sample was considered as at a medium risk of food contamination (46.5% of them presented from 51 to 75% of conformities to the instrument). There was a statistical difference between house-kitchens with a family income from zero to one minimum wage (MW) and those receiving from 5 to 15 MW (p = 0.017), as well as between those from zero to one MW and who earn above 15 MW (p = 0.009). The result of the on-site evaluation shows that the instrument was able to measure food safety conditions in Brazilian Federal District domestic kitchens. Such findings can contribute positively to the development of actions in health education that help in the adoption of good practices of food manipulation and, consequently, in the reduction in foodborne disease outbreaks in residences.
Effects of Neuromuscular Electrical Stimulation Synchronized with Chewing Exercises on Bite Force and Masseter Muscle Thickness in Community-Dwelling Older Adults in South Korea: A Randomized Controlled Trial
Authors: Park Ji-Su , Jung Young-Jin , Kim Min-Ji
Published: July 2020

This study is aimed at investigating the effects of synchronized neuromuscular electrical stimulation (NMES) and chewing exercises on bite force and the masseter muscle thickness in community-dwelling older adults. Material and methods: Forty older adults were enrolled in South Korea and randomly assigned to either an experimental or control group. The experimental group performed chewing exercises using the No-Sick Exerciser equipment synchronized with NMES applied to the bilateral masseter muscles, while the control group performed only chewing exercises. Both groups received interventions for 20 min/day, 5 days/week, for 6 weeks. Bite force was measured using the OCCLUZER device, and masseter muscle thickness was measured using a portable ultrasound. Results: Both groups showed a significant increase in bite force and masseter muscle thickness compared to baseline measurements (p < 0.05). The experimental group showed a significantly higher increase in bite force and masseter muscle thickness than the control group after combined intervention (p < 0.05). Conclusion: This study demonstrates that NMES synchronized with chewing exercises is more efficient in increasing bite force and masseter muscle thickness than chewing exercises alone in community-dwelling older adults.
Health Literacy of Osteoporosis Risks among Caregivers Serving in Disability Care Facilities
Authors: Lin Lan-Ping , Lai Wei-Ju , Hsu Shang-Wei , Lin Jin-Ding
Published: July 2020

Osteoporosis is a global public health issue and its consequent effects are a growing concern worldwide. Caregivers generally experience occupational physical ailments and they have less of a tendency to engage in preventive health behaviors, leading them to be in a higher risk group for osteoporosis. This study aims to present a general profile of health literacy related to osteoporosis risks and identify its associated factors among disability institutional caregivers. A cross-sectional study with a structured questionnaire was used to collect information on 465 caregivers from seven disability care institutions regarding their awareness of the health literacy related to osteoporosis risks. The results indicate that the average literacy score related to osteoporosis risks among the respondents was 60 points (full score is 80 points), with 50–59 being the most common range (51.9%), followed by 60–69 points (43.5%), and 4.4% of cases had more than 70 points. A multivariate logistic regression model revealed that respondents’ age (40–49 vs. 18–29; odds ratio (OR) = 2.53, 95% confidence interval (CI) = 1.31–4.87), education level (senior high vs. primary and junior high, OR = 2.00, 95% CI = 1.03–3.89; college and above vs. primary and junior high, OR = 3.66, 95% CI = 1.84–7.31), experience in undergoing a bone density test (OR = 1.94, 95% CI = 1.28–2.93), and poor physical fitness status (OR = 0.64, 95% CI = 0.43–0.95) were the significant predictors of the osteoporosis health literacy level. The osteoporosis health literacy of institutional caregivers is moderate, and there are many items that are worthy of attention in future health promotion programs. This study highlights risk factors related to a lower level of osteoporosis healthy literacy such as older age, less education, no experience of bone density test, and poor physical fitness that highlight the need to raise further awareness in order to improve caregivers’ bone health.
Theoretical Investigations on Interactions of Arylsulphonyl Indazole Derivatives as Potential Ligands of VEGFR2 Kinase
Authors: Czaja Kornelia , Kujawski Jacek , Śliwa Paweł , Kurczab Rafał , Kujawski Radosław , Stodolna Anna , Myślińska Agnieszka , Bernard Marek K.
Published: July 2020

Vascular endothelial growth factor receptor 2 (VEGFR2) is a key receptor in the angiogenesis process. The VEGFR2 expression is upregulated in many cancers so this receptor is an important target for anticancer agents. In the present paper, we analyse interactions of several dimeric indazoles, previously investigated for anticancer activity, with the amino acids present in the VEGFR2 binding pocket. Using the docking method and MD simulations as well as theoretical computations (SAPT0, PIEDA, semi-empirical PM7), we confirmed that these azoles can efficiently bind into the kinase pocket and their poses can be stabilised by the formation of hydrogen bonds, π–π stacking, π–cation, and hybrid interactions with some amino acids of the kinase cavity like Ala866, Lys868, Glu885, Thr916, Glu917, and Phe918.
Integrative Analyses of Widely Targeted Metabolic Profiling and Transcriptome Data Reveals Molecular Insight into Metabolomic Variations during Apple (Malus domestica) Fruit Development and Ripening
Authors: Xu Jidi , Yan Jinjiao , Li Wenjie , Wang Qianying , Wang Caixia , Guo Junxing , Geng Dali , Guan Qingmei , Ma Fengwang
Published: July 2020

The apple is a favorite fruit for human diet and is one of the most important commercial fruit crops around the world. Investigating metabolic variations during fruit development can provide a better understanding on the formation of fruit quality. The present study applied a widely targeted LC-MS-based metabolomics approach with large-scale detection, identification and quantification to investigate the widespread metabolic changes during “Pinova” apple development and ripening. A total of 462 primary and secondary metabolites were simultaneously detected, and their changes along with the four fruit-development stages were further investigated. The results indicated that most of the sugars presented increasing accumulation levels while organic acid, including Tricarboxylic acid cycle (TCA) intermediates, showed a distinct decreasing trend across the four fruit-development stages. A total of 207 secondary metabolites consisted of 104 flavonoids and 103 other secondary metabolites. Many flavonoids maintained relatively high levels in the early fruit stage and then rapidly decreased their levels at the following developmental stages. Further correlation analyses of each metabolite–metabolite pair highlighted the cross talk between the primary and secondary metabolisms across fruit development and ripening, indicating the significant negative correlations between sugars and secondary metabolites. Moreover, transcriptome analysis provided the molecular basis for metabolic variations during fruit development. The results showed that most differentially expressed genes (DEGs) involved in the TCA cycle were upregulated from the early fruit stage to the preripening stage. The extensive downregulation of controlling genes involved in the flavonoid pathway is probably responsible for the rapid decrease of flavonoid content at the early fruit stage. These data provide a global view of the apple metabolome and a comprehensive analysis on metabolomic variations during fruit development, providing a broader and better understanding on the molecular and metabolic basis of important fruit quality traits in commercial apples.
Neutrophil Extracellular Traps Generation Relates with Early Stage and Vascular Complications in Systemic Sclerosis
Authors: Didier Kevin , Giusti Delphine , Le Jan Sebastien , Terryn Christine , Muller Celine , Pham Bach Nga , Le Naour Richard , Antonicelli Frank D. , Servettaz Amelie
Published: July 2020

Systemic sclerosis (SSc) is a systemic disease characterized by a great clinical and immunological heterogeneity whose pathophysiology is still being unraveled. Recently, innate immunity has been proposed to participate to the pathogenesis of SSc. In this study, we investigated the release of neutrophil extracellular traps (NETs) according to patient phenotype. Polymorphonuclear neutrophils (PMN) from 34 SSc patients and 26 healthy controls were stimulated by serum from SSc or healthy subject. NETs were visualized using epifluorescence microscope after DNA, myeloperoxidase, and Histone H3 tagging. Area of NETs were quantified using an original macro running in ImageJ® software. PMN from SSc patients were significantly more prone to releasing NETs than control PMN after autologous stimulation. PMN from patients with severe vascular complications (pulmonary arterial hypertension, digital ulcers) produced more NETs than PMN from other SSc patients and their aberrant NET production appeared to be sustained over time. In patients with pulmonary interstitial disease or extensive cutaneous fibrosis, NET production was high at an early stage of the disease before progressively decreasing. Both serum factors and PMN activation status were involved in the enhanced production of NETs in SSc. Consequently, neutrophils and especially NETosis represent new physiopathological and therapeutic fields in SSc.
The Impact of the 2019 European Guideline for Cardiovascular Risk Management: A Cross-Sectional Study in General Practice
Authors: Meier Rahel , Rachamin Yael , Rosemann Thomas , Markun Stefan
Published: July 2020

The aim of this study was to assess the impact of the 2019 published European Society of Cardiology (ESC)/European Atherosclerosis Society (EAS) guideline on cardiovascular (CV) risk management compared with its predecessor from 2016 in a cohort in general practice. We performed a cross-sectional retrospective study with data from electronic medical records. The study cohort included 103,351 patients with known CV risk. We assessed changes in CV risk classification and low-density lipoprotein cholesterol (LDL-C) target values, the impact on LDL-C achievement rates, and the current lipid-lowering treatments. Under the 2019 ESC guideline, CV risk categories changed in 27.5% of patients, LDL-C target levels decreased in 71.4% of patients, and LDL-C target achievement rate dropped from 31.1% to 16.5%. Among non-achievers according to the 2019 guideline, 52.2% lacked lipid-lowering drugs entirely, and 41.5% had conventional drugs at a submaximal intensity. Of patients in the high-risk and very high-risk categories, at least 5% failed to achieve the LDL-C target level despite treatment at maximal intensity with conventional lipid-lowering drugs, making them eligible for PCSK-9 inhibitors. In conclusion, the 2019 ESC/EAS guideline lowered LDL-C target values for the majority of patients in general practice and halved LDL-C target achievement rates. There is still a large undeveloped potential to lower CV risk by introducing conventional lipid-lowering drugs, particularly in patients at high or very high CV risk. A substantial proportion of the patients can only achieve their LDL-C targets using PCSK-9 inhibitors, which would currently require an at least 10-fold increase in prescribing of these drugs.
Ehlers–Danlos Syndrome and Hypermobility Syndrome Compared with Other Common Chronic Pain Diagnoses—A Study from the Swedish Quality Registry for Pain Rehabilitation
Authors: Molander Peter , Novo Mehmed , Hållstam Andrea , Löfgren Monika , Stålnacke Britt-Marie , Gerdle Björn
Published: July 2020

Although chronic pain is common in patients with Ehlers–Danlos syndrome (EDS) and hypermobility syndromes (HMS), little is known about the clinical characteristics of these groups. The main aim was to compare EDS/HMS with common local and generalized pain conditions with respect to Patient Reported Outcome Measures (PROMs). Data from the Swedish Quality Register for Chronic Pain (SQRP) from 2007 to 2016 (n = 40,518) were used, including patients with EDS/HMS (n = 795), fibromyalgia (n = 5791), spinal pain (n = 6693), and whiplash associated disorders (WAD) (n = 1229). No important differences in the PROMs were found between EDS and HMS. Women were represented in > 90% of EDS/HMS cases and fibromyalgia cases, and in about 64% of the other groups. The EDS/HMS group was significantly younger than the others but had a longer pain duration. The pain intensity in EDS/HMS was like those found in spinal pain and WAD; fibromyalgia had the highest pain intensity. Depressive and anxiety symptoms were very similar in the four groups. Vitality—a proxy for fatigue—was low both in EDS/HMS and fibromyalgia. The physical health was lower in EDS/HMS and fibromyalgia than in the two other groups. Patients with EDS/HMS were younger, more often female, and suffered from pain for the longest time compared with patients who had localized/regional pain conditions. Health-care clinicians must be aware of these issues related to EDS/HMS both when assessing the clinical presentations and planning treatment and rehabilitation interventions.
Investigating Optimal Time Step Intervals of Imaging for Data Quality through a Novel Fully-Automated Cell Tracking Approach
Authors: Yang Feng Wei , Tomášová Lea , Guttenberg Zeno v. , Chen Ke , Madzvamuse Anotida
Published: July 2020

Computer-based fully-automated cell tracking is becoming increasingly important in cell biology, since it provides unrivalled capacity and efficiency for the analysis of large datasets. However, automatic cell tracking’s lack of superior pattern recognition and error-handling capability compared to its human manual tracking counterpart inspired decades-long research. Enormous efforts have been made in developing advanced cell tracking packages and software algorithms. Typical research in this field focuses on dealing with existing data and finding a best solution. Here, we investigate a novel approach where the quality of data acquisition could help improve the accuracy of cell tracking algorithms and vice-versa. Generally speaking, when tracking cell movement, the more frequent the images are taken, the more accurate cells are tracked and, yet, issues such as damage to cells due to light intensity, overheating in equipment, as well as the size of the data prevent a constant data streaming. Hence, a trade-off between the frequency at which data images are collected and the accuracy of the cell tracking algorithms needs to be studied. In this paper, we look at the effects of different choices of the time step interval (i.e., the frequency of data acquisition) within the microscope to our existing cell tracking algorithms. We generate several experimental data sets where the true outcomes are known (i.e., the direction of cell migration) by either using an effective chemoattractant or employing no-chemoattractant. We specify a relatively short time step interval (i.e., 30 s) between pictures that are taken at the data generational stage, so that, later on, we may choose some portion of the images to produce datasets with different time step intervals, such as 1 min, 2 min, and so on. We evaluate the accuracy of our cell tracking algorithms to illustrate the effects of these different time step intervals. We establish that there exist certain relationships between the tracking accuracy and the time step interval associated with experimental microscope data acquisition. We perform fully-automatic adaptive cell tracking on multiple datasets, to identify optimal time step intervals for data acquisition, while at the same time demonstrating the performance of the computer cell tracking algorithms.
A New Model of the Personnel Function Delivery in the Logistics of Polish Firms
Authors: Gołembska Elżbieta , Gołembski Marcin
Published: July 2020

In the article, a new phenomenon of global logistification provides a context for presenting the newest trends in the development of HR management and its role in logistics. The new trends include a growing internationalization of firms, combined flows of capital, people and technical knowledge, and the issue of key logistics competences. Against this background, the article describes a new model of the HR management function in logistics. The aim is to find the relationship between designing and delivering the personnel function in a firm and the effectiveness of logistics management. The key aspects of the personnel function management include talent and competencies management, personnel risk management, and employer branding. These HR practices are explored to determine attributes of the personnel function, and to describe challenges of the proactive human resource management in logistics. The article continues with a presentation of the results of empirical studies conducted in the period 2017–2018 of 236 large, medium-sized, and small Polish firms. As the primary methods, the research used a diagnostic survey, a questionnaire, and the statistical method of factor analysis. The second part of the article presents an original model of the personnel function in logistics management, with regard to the specificity of Polish firms. The findings based on the study results refer to both the present and future status of HR management in logistics. The main findings of the studies acknowledge the influence of all-embracing logistification on the volumes and structure of knowledge transfer in supply chains, and reveal a growing decentralization of HR management in large and medium-sized companies, resulting in an imbalance between the levels of personnel function implementation in large and medium-sized companies, compared with small firms.
Multiscale Characterizations of Surface Anisotropies
Authors: Bartkowiak Tomasz , Berglund Johan , Brown Christopher A.
Published: July 2020

Anisotropy can influence surface function and can be an indication of processing. These influences and indications include friction, wetting, and microwear. This article studies two methods for multiscale quantification and visualization of anisotropy. One uses multiscale curvature tensor analysis and shows anisotropy in horizontal coordinates i.e., topocentric. The other uses multiple bandpass filters (also known as sliding bandpass filters) applied prior to calculating anisotropy parameters, texture aspect ratios (Str) and texture directions (Std), showing anisotropy in horizontal directions only. Topographies were studied on two milled steel surfaces, one convex with an evident large scale, cylindrical form anisotropy, the other nominally flat with smaller scale anisotropies; a µEDMed surface, an example of an isotropic surface; and an additively manufactured surface with pillar-like features. Curvature tensors contain the two principal curvatures, i.e., maximum and minimum curvatures, which are orthogonal, and their directions, at each location. Principal directions are plotted for each calculated location on each surface, at each scale considered. Histograms in horizontal coordinates show altitude and azimuth angles of principal curvatures, elucidating dominant texture directions at each scale. Str and Std do not show vertical components, i.e., altitudes, of anisotropy. Changes of anisotropy with scale categorically failed to be detected by traditional characterization methods used conventionally. These multiscale methods show clearly in several representations that anisotropy changes with scale on actual surface measurements with markedly different anisotropies.
Dynamic Mechanical Response and Damage Mechanism of HTPB Propellant under Impact Loading
Authors: Zhang Hengning , Liu Meng , Miao Yinggang , Wang Han , Chen Tao , Fan Xuezhong , Chang Hai
Published: July 2020

The dynamic mechanical behaviors of Hydroxyl-terminated polybutadiene (HTPB) propellant was studied by a split Hopkinson pressure bar apparatus (SHPB) at strain rates ranging from 103 to 104 s−1. The obtained stress–strain curves indicated that the mechanical features, such as ultimate stress and strain energy, were strongly dependent on the strain rate. The real time deformation and fracture evolution of HTPB propellant were captured by a high-speed digital camera accompanied with an SHPB setup. Furthermore, microscopic observation for the post-test specimen was conducted to explore the different damage mechanisms under various conditions of impact loading. The dominated damage characteristics of HTPB propellant were changed from debonding and matrix tearing to multiple cracking modes of ammonium perchlorate (AP) particles, along with the increase of the strain rate. For the first time, the influence of AP particle density on the dynamic response of HTPB propellant was studied by analyzing the strain-rate sensitivity (SRS) index of HTPB propellant with two different filler content (80 wt.% and 85 wt.%), which deduced from a power function of ultimate stress and strain energy density. The result of this study is of significance for evaluating the structural integrity and security of HTPB propellant.
A Facile Synthesis and Characterization of Highly Crystalline Submicro-Sized BiFeO3
Authors: Karoblis Dovydas , Griesiute Diana , Mazeika Kestutis , Baltrunas Dalis , Karpinsky Dmitry V. , Lukowiak Anna , Gluchowski Pawel , Raudonis Rimantas , Katelnikovas Arturas , Zarkov Aleksej , Kareiva Aivaras
Published: July 2020

In this study, a highly crystalline bismuth ferrite (BFO) powder was synthesized using a novel, very simple, and cost-effective synthetic approach. It was demonstrated that the optimal annealing temperature for the preparation of highly-pure BFO is 650 °C. At lower or higher temperatures, the formation of neighboring crystal phases was observed. The thermal behavior of BFO precursor gel was investigated by thermogravimetric and differential scanning calorimetry (TG-DSC) measurements. X-ray diffraction (XRD) analysis and Mössbauer spectroscopy were employed for the investigation of structural properties. Scanning electron microscopy (SEM) was used to evaluate morphological features of the synthesized materials. The obtained powders were also characterized by magnetization measurements, which showed antiferromagnetic behavior of BFO powders.
Parallel Matrix-Free Higher-Order Finite Element Solvers for Phase-Field Fracture Problems
Authors: Jodlbauer Daniel , Langer Ulrich , Wick Thomas
Published: July 2020

Phase-field fracture models lead to variational problems that can be written as a coupled variational equality and inequality system. Numerically, such problems can be treated with Galerkin finite elements and primal-dual active set methods. Specifically, low-order and high-order finite elements may be employed, where, for the latter, only few studies exist to date. The most time-consuming part in the discrete version of the primal-dual active set (semi-smooth Newton) algorithm consists in the solutions of changing linear systems arising at each semi-smooth Newton step. We propose a new parallel matrix-free monolithic multigrid preconditioner for these systems. We provide two numerical tests, and discuss the performance of the parallel solver proposed in the paper. Furthermore, we compare our new preconditioner with a block-AMG preconditioner available in the literature.
Investigation of Corrosion Behaviors on an Fe/Cu-Type ACM Sensor under Various Environments
Authors: Pei Zibo , Xiao Kui , Chen Lihong , Li Qing , Wu Jun , Ma Lingwei , Li Xiaogang
Published: July 2020

The applicability of galvanic-cell-based atmospheric corrosion monitoring (ACM) technology has been confirmed empirically in field tests, however the corrosion behaviors on the ACM sensors have rarely been studied systematically. In this study, the influence of temperature, chloride ions, and hydrosulfite (simulated sulfur dioxide) ions on the corrosion behaviors of Fe/Cu-type ACM sensors was investigated. The results show that the hydrosulfite ions led to a larger increase in the Fe/Cu-based ACM current than chloride ions in the initial stage of corrosion, and both changed the components of the corrosion products. Moreover, the hydrosulfite and chloride ions showed a synergistic effect on the corroded ACM sensor. Lastly, a positive correlation between ACM technology and the mass loss method was observed, further indicating that ACM technology can be an effective, convenient, and fast approach to studying the accelerated corrosion behaviors of steels.
Early Perturbations in Glucose Utilization in Malaria-Infected Murine Erythrocytes, Liver and Brain Observed by Metabolomics
Authors: Sengupta Arjun , Ghosh Soumita , Sharma Shobhona , Sonawat Haripalsingh M.
Published: July 2020

Investigation of glucose utilization during an infection is central to the study of energy metabolism. The heavy utilization of glucose by the malaria parasite, and the consequences of this process, have been investigated extensively. However, host glucose utilization during early infection has not been explored to date. In a first attempt, this article investigates the changes in the host glucose utilization in Balb/c mice infected with Plasmodium berghei ANKA using 13C-labeled glucose infusion followed by NMR spectroscopy. The results suggested significant alterations of liver, brain and red blood cell (RBC) glucose utilization during early infection when the parasitemia was <1%. At the pathway level, we observed a decrease in the shunt metabolite 2,3-bisphosphoglycerate in the RBCs. Glycolysis and pathways associated with it, along with fatty acid unsaturation, were altered in the liver. Significant changes were observed in the central carbon metabolic pathways in the brain. These results have implications in understanding the host physiology during early infection and pave the way for detailed flux analysis of the proposed perturbed pathways.
Transcriptomic and Ultrastructural Signatures of K+-Induced Aggregation in Phytophthora parasitica Zoospores
Authors: Bassani Ilaria , Rancurel Corinne , Pagnotta Sophie , Orange François , Pons Nicolas , Lebrigand Kevin , Panabières Franck , Counillon Laurent , Noblin Xavier , Galiana Eric
Published: July 2020

Most pathogenic oomycetes of the genus Phytophthora spread in water films as flagellated zoospores. Zoospores perceive and produce signals attracting other zoospores, resulting in autoaggregation in vitro or biofilm formation on plant surface. The mechanisms underlying intercellular communication and consequent attraction, adhesion and aggregation are largely unknown. In Phytophthora parasitica, the perception of a K+ gradient induces coordinated motion and aggregation. To define cellular and molecular events associated with oomycete aggregation, we combined transcriptomic and ultrastructural analyses. Results indicate involvement of electroception in K+ sensing. They establish that the transcriptome repertoire required for swimming and aggregation is already fully functional at zoospore release. At the time points analyzed, aggregates are mainly constituted of zoospores. They produce vesicular and fibrillary material discharged at cell-to-cell contacts. Consistently, the signature of transcriptome dynamics during transition to aggregates is an upregulation of genes potentially related to vesicular trafficking. Moreover, transcriptomic and functional analyses show a strong enhancement of carbonic anhydrase activity, indicating that pH homeostasis may contribute to aggregation by acting on both zoospore movement and adhesion. This study poses the molecular and cellular bases of aggregative behavior within oomycetes and expands the current knowledge of ion perception-mediated dissemination of propagules in the rhizosphere.
Protein Intake and Exercise-Induced Skeletal Muscle Hypertrophy: An Update
Authors: Deldicque Louise
Published: July 2020

Skeletal muscle mass is critical for sport performance and in many pathological conditions. The combination of protein intake and resistance exercise is the most efficient strategy to promote skeletal muscle hypertrophy and remodeling. However, to be really efficient, certain conditions need to be considered. The amount, type and source of proteins do all matter as well as the timing of ingestion and spreading over the whole day. Optimizing those conditions favor a positive net protein balance, which in the long term, may result in muscle mass accretion. Last but not least, it is also essential to take the nutritional status and the exercise training load into consideration when looking for maintenance or gain of skeletal muscle mass.