bluuebunny/crossref_metadata_2025 huggingface dataset:
Aspects of Self-Organization of Tribological Stressed Lubricating Greases
Authors: Kuhn Erik
Published: March 2020

Lubricating greases are markedly visco-elastic materials. Stressed by a friction process, this special material shows a drop of a measured shear stress or viscosity. This typical behaviour is observed in a number of papers and, therefore, is well known. Some different explanations can be found but most of them describe a structural degradation caused by the friction process. This paper attempts to elucidate the conditions that promote that structural change and understand this behaviour as an intrinsic response of the system.
Application of a Novel CVD TiN Coating on a Biomedical Co–Cr Alloy: An Evaluation of Coating Layer and Substrate Characteristics
Authors: Song Si Hoon , Min Bong Ki , Hong Min-Ho , Kwon Tae-Yub
Published: March 2020

Titanium nitride (TiN) was deposited on the surface of a cobalt–chromium (Co–Cr) alloy by a hot-wall type chemical vapor deposition (CVD) reactor at 850 °C, and the coating characteristics were compared with those of a physical vapor deposition (PVD) TiN coating deposited on the same alloy at 450 °C. Neither coating showed any reactions at the interface. The face-centered cubic (fcc) structure of the alloy was changed into a hexagonal close-packed (hcp) phase, and recrystallization occurred over at 10 μm of depth from the surface after CVD coating. Characteristic precipitates were also generated incrementally depending on the depth, unlike the precipitates in the matrix of the as-cast alloy. On the other hand, the microstructure and phase of the PVD-coated alloy did not change. Depth-dependent nano-hardness measurements showed a greater increase in hardness in the recrystallization zone of the CVD-coated alloy than in the bulk center of the alloy. The CVD coating showed superior adhesion to the PVD coating in the progressive scratch test. The as-cast, PVD-coated, and CVD-coated alloys all showed negative cytotoxicity. Within the limitations of this study, CVD TiN coating to biomedical Co–Cr alloy may be considered a promising alternative to PVD technique.
Reactive Compatibilization of Polyamide 6/Olefin Block Copolymer Blends: Phase Morphology, Rheological Behavior, Thermal Behavior, and Mechanical Properties
Authors: Lin Xintu , Liu Yuejun , Chen Xi , Wu Yincai , Cui Lingna , Mao Long , Zheng Wei , Lin Minghao
Published: March 2020

In this study, the morphology, rheological behavior, thermal behavior, and mechanical properties of a polyamide 6 (PA6) and olefin block copolymer (OBC) blend compatibilized with maleic anhydride-grafted polyethylene-octene copolymer (POE-g-MAH) were investigated. The morphological observations showed that the addition of POE-g-MAH enhanced the OBC particle dispersion in the PA6 matrix, suggesting a better interfacial compatibility between the pure PA6 and OBC. The results of the Fourier transform infrared (FTIR) spectroscopy analysis and the Molau test confirmed the compatibilization reactions between POE-g-MAH and PA6. The rheological test revealed that the melt viscosity, storage modulus (G’), and loss modulus (G”) of the compatibilized PA6/OBC blends at low frequency were increased with the increasing POE-g-MAH content. The thermal analysis indicated that the addition of OBC had little effect on the crystallization behavior of PA6, while the incorporation of POE-g-MAH at high content (7 wt%) in the PA6/OBC blend restricted the crystallization of PA6. In addition, the compatibilized blends exhibited a significant enhancement in impact strength compared to the uncompatibilized PA6/OBC blend, in which the highest value of impact strength obtained at a POE-g-MAH content of 7 wt% was about 194% higher than that of pure PA6 under our experimental conditions.
Numerical Investigation on Lateral Confinement Effects on Concrete Cracking Induced by Rebar Corrosion
Authors: Choe Gyeongcheol , Shinohara Yasuji , Kim Gyuyong , Nam Jeongsoo
Published: March 2020

Accelerated corrosion tests of reinforced concrete (RC) specimens were conducted to estimate the corrosion expansion rate of reinforcing bars. Subsequently, finite element analysis was performed with the estimated expansion rate for RC beams to investigate concrete cracking induced by corrosion. The influence of the different confinement levels on crack behavior was investigated using mainly the amount of transverse reinforcement. An expansion rate of 2 was found to be appropriate when using Lundgren’s expansion model. Confinement levels affected the cracking behavior of steel bars. Cracks did not significantly affect structural capacity although they exceeded the allowable crack width. Nevertheless, repair and reinforcement measures are necessary because degrading durability factors such as carbonation or salt diffusion can reach the reinforcing bars through connected cracks.
Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus
Authors: Hoshino Roberto Alameda , Silva Guilherme Ferreira da , Delfino Mateus Machado , Guerreiro-Tanomaru Juliane Maria , Tanomaru-Filho Mario , Sasso-Cerri Estela , Bonetti Filho Idomeo , Cerri Paulo Sérgio
Published: March 2020

We investigated the physical properties, antimicrobial activity, and tissue reaction to Apexit Plus in comparison to Sealapex. Flow, radiopacity, setting time, and solubility were evaluated in each material. The antimicrobial activity against Enterococcus faecalis was performed. Polyethylene tubes containing Apexit Plus or Sealapex, and without material (control group) were implanted into the subcutaneous tissue of rats. At 7, 15, 30, and 60 days of implantation, the specimens were paraffin-embedded and the number of inflammatory cells (ICs) and the amount of birefringent collagen (BC) were quantified. The von Kossa reaction followed by immunohistochemistry for detection of alkaline phosphatase (ALP) was also performed. Statistical analysis was performed with ANOVA and Tukey test (p ≤ 0.05). The flow value of Apexit Plus was greater than Sealapex, whereas the radiopacity (3.44 mm Al) was lower than Sealapex (6.82 mm Al). Apexit Plus showed lower solubility and shorter initial and final setting (p < 0.0001), whereas the antimicrobial activity was significantly greater than Sealapex. Although the number of ICs was higher in Apexit Plus (p = 0.0009) at 7 days, no significant difference was detected between Apexit Plus and Sealapex at 15, 30, and 60 days. All groups showed higher values for BC in the capsules over time. ALP-immunolabelled cells were observed, mainly around von Kossa-positive structures, either in the capsules of Apexit Plus or Sealapex. Therefore, our results revealed that Apexit Plus exhibited a greater effectiveness against Enterococcus faecalis and better physical properties than Sealapex, except for the radiopacity. In vivo findings indicate that Apexit Plus is biocompatible and presents potential bioactivity in the subcutaneous tissue.
Dehydration of Fructose to 5-HMF over Acidic TiO2 Catalysts
Authors: Testa Maria Luisa , Miroddi Gianmarco , Russo Marco , La Parola Valeria , Marcì Giuseppe
Published: March 2020

Different solid sulfonic titania-based catalysts were investigated for the hydrothermal dehydration of fructose to 5-hydroxymethylfurfural (5-HMF). The catalytic behavior of the materials was evaluated in terms of fructose conversion and selectivity to 5-HMF. The surface and structural properties of the catalysts were investigated by means of X-ray diffraction (XRD), N2 adsorption isotherms, thermo-gravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and acid capacity measurements. Special attention was focused on the reaction conditions, both in terms of 5-HMF selectivity and the sustainability of the process, choosing water as the solvent. Among the various process condition studied, TiO2-SO3H catalyzed a complete conversion (99%) of 1.1M fructose and 5-HMF selectivity (50%) and yield (50%) at 165 °C. An important improvement of the HMF selectivity (71%) was achieved when the reaction was carried out by using a lower fructose concentration (0.1M) and lower temperature (140 °C). The catalytic activities of the materials were related to their acid capacities as much as their textural properties. In particular, a counterbalance between the acidity and the structure of the pores in which the catalytic sites are located, results in the key issue for switch the selectivity towards the achievement of 5-HMF.
Experimental Investigation on Machinability of Polypropylene Reinforced with Miscanthus Fibers and Biochar
Authors: Tran Dinh Son , Songmene Victor , Ngo Anh Dung , Kouam Jules , Rodriguez-Uribe Arturo , Misra Manjusri , Mohanty Amar Kumar
Published: March 2020

The machinability of composite materials depends on reinforcements, matrix properties, cutting parameters, and on the cutting tool used (material, coating, and geometry). For new composites, experimental studies must be performed in order to understand their machinability, and thereby help manufacturers establishing appropriate cutting data. In this study, investigations are conducted to analyze the effects of cutting parameters and drill bit diameter on the thrust force, surface roughness, specific cutting energy, and dust emission during dry drilling of a new hybrid biocomposite consisting of polypropylene reinforced with miscanthus fibers and biochar. A full factorial design was used for the experimental design. It was found that the feed rate, the spindle speed, and the drill bit diameter have significant effects on the thrust force, the surface roughness, and the specific cutting energy. The effects of the machining parameters and the drill bit diameter on ultrafine particles emitted were not statistically significant, while the feed rate and drill bit diameter had significant effects on fine particle emission.
Precipitation Strengthening of Cu–Ni–Si Alloy
Authors: Krupińska Beata , Rdzawski Zbigniew , Krupiński Mariusz , Pakieła Wojciech
Published: March 2020

The work examines the effect of rhenium addition on the structure and properties of Cu–2Ni–1Si alloys. The aim of this work was to answer the question of how the addition of rhenium will affect the strengthening mechanisms of rhenium-modified, saturated, plastically deformed and aged Cu–2Ni–1Si alloys. How will this affect the crystallization process? What effect will it have on the properties? Scanning electron microscopy (SEM) and analysis of chemical composition in microareas (energy-dispersive X-ray spectroscopy, EDS), light microscopy, measurements of microhardness and conductivity of the alloys were used for the investigations. Research on chemical and phase composition were carried out with application of transmission electron microscopy (TEM), and scanning transmission electron microscopy (STEM). Modification with rhenium has caused an increase in hardness as a result of precipitation of small phases with rhenium. As the effect of supersaturation, cold plastic treatment as well as aging small phases with rhenium with a size of 200 nm to 600 nm causes both reinforcement of the alloy and makes recrystallization impossible. Re-addition also influences the stabilization of the structure.
Some Notes on the Formation of a Pair in Pairs Trading
Authors: Ramos-Requena José Pedro , Trinidad-Segovia Juan Evangelista , Sánchez-Granero Miguel Ángel
Published: March 2020

The main goal of the paper is to introduce different models to calculate the amount of money that must be allocated to each stock in a statistical arbitrage technique known as pairs trading. The traditional allocation strategy is based on an equal weight methodology. However, we will show how, with an optimal allocation, the performance of pairs trading increases significantly. Four methodologies are proposed to set up the optimal allocation. These methodologies are based on distance, correlation, cointegration and Hurst exponent (mean reversion). It is showed that the new methodologies provide an improvement in the obtained results with respect to an equal weighted strategy.
The Stability and Stabilization of Infinite Dimensional Caputo-Time Fractional Differential Linear Systems
Authors: Zitane Hanaa , Boutoulout Ali , Torres Delfim F. M.
Published: March 2020

We investigate the stability and stabilization concepts for infinite dimensional time fractional differential linear systems in Hilbert spaces with Caputo derivatives. Firstly, based on a family of operators generated by strongly continuous semigroups and on a probability density function, we provide sufficient and necessary conditions for the exponential stability of the considered class of systems. Then, by assuming that the system dynamics are symmetric and uniformly elliptical and by using the properties of the Mittag–Leffler function, we provide sufficient conditions that ensure strong stability. Finally, we characterize an explicit feedback control that guarantees the strong stabilization of a controlled Caputo time fractional linear system through a decomposition approach. Some examples are presented that illustrate the effectiveness of our results.
The Euler-Equation Approach in Average-Oriented Opinion Dynamics
Authors: Mazalov Vladimir , Parilina Elena
Published: March 2020

We consider the models of average-oriented opinion dynamics. An opinion about an event is distributed among the agents of a social network. There are an optimization problem and two game-theoretical models when players as centers of influence aim to make the opinions of the agents closer to the target ones in a finite time horizon minimizing their costs. The optimization problem and the games of competition for the agents’ opinion are linear-quadratic and solved using the Euler-equation approach. The optimal strategies for optimization problem and the Nash equilibria in the open-loop strategies for the games are found. Numerical simulations demonstrate theoretical results.
Self-Regulating Artificial-Free Linear Programming Solver Using a Jump and Simplex Method
Authors: Visuthirattanamanee Rujira , Sinapiromsaran Krung , Boonperm Aua-aree
Published: March 2020

An enthusiastic artificial-free linear programming method based on a sequence of jumps and the simplex method is proposed in this paper. It performs in three phases. Starting with phase 1, it guarantees the existence of a feasible point by relaxing all non-acute constraints. With this initial starting feasible point, in phase 2, it sequentially jumps to the improved objective feasible points. The last phase reinstates the rest of the non-acute constraints and uses the dual simplex method to find the optimal point. The computation results show that this method is more efficient than the standard simplex method and the artificial-free simplex algorithm based on the non-acute constraint relaxation for 41 netlib problems and 280 simulated linear programs.
Effects of Unequal Lot Size and Variable Transportation in Unreliable Supply Chain Management
Authors: Hota Soumya Kanti , Sarkar Biswajit , Ghosh Santanu Kumar
Published: March 2020

The effect of unreliable players on the supply chain management with a single-setup-multi-unequal-increasing-delivery-policy (SSMUID) along with a service-dependent demand and investment is discussed in this model. The manufacturer is unreliable which causes an increase of lead time and shortage. For solving the shortage problem and reducing lead time crashing cost (LTCC), an investment is utilized with the variable backorder price discounts. The number of transportation increases due to the new transportation policy and it causes pollution. Besides the fixed transportation and carbon emission cost (FTCEC), a container dependent carbon emission cost is applied. Some investments for setup cost reduction (SCR), ordering cost reduction (OCR), and quality improvement (QI) are considered. The lead time demand follows a normal distribution. The total cost of the supply chain is optimized and the model is tested numerically. The main intent of this study is to solve the shortage problem which occurs due to unreliability of the manufacturer. The study helps to reduce the unreliability issue of the manufacturer. The objective function is solved by using the classical optimization technique. Numerical results show that the discount for partial backorder enhances the profitability of the manufacturer. The sensitiveness of the parameters are discussed through the sensitivity of analysis and some special cases. Managerial insights provide the applicability of this study among different sectors.
Midpalatal Suture Density Evaluation after Rapid and Slow Maxillary Expansion with a Low-Dose CT Protocol: A Retrospective Study
Authors: Fastuca Rosamaria , Michelotti Ambra , Nucera Riccardo , D’Antò Vincenzo , Militi Angela , Logiudice Antonino , Caprioglio Alberto , Portelli Marco
Published: March 2020

Background and objectives: The aim of the present paper is to use low-dose computed tomography (CT) to evaluate the changes in the midpalatal suture density in patients treated with rapid maxillary expansion (RME) and slow maxillary expansion (SME). Materials and Methods: Thirty patients (mean age 10.2 ± 1.2 years) were retrospectively selected from the existing sample of a previous study. For each patient, a low-dose computed tomography examination was performed before appliance placement (T0) and at the end of retention (T1), seven months later. Using the collected images, the midpalatal suture density was evaluated in six regions of interest. Results: No significant differences were found between the timepoints in the rapid maxillary expansion group. Three out of six regions of interest showed significant decreases between the timepoints in the slow maxillary expansion group. No significant differences were found in comparisons between the two groups. Conclusions: The midpalatal suture density showed no significant differences when rapid maxillary expansion groups were compared to slow maxillary expansion groups, suggesting that a similar rate of suture reorganization occurs despite different expansion protocols.
Non-Alcoholic Cirrhosis and Heart Rate Variability: A Systematic Mini-Review
Authors: Anaize Tonon do Amaral Joice , Salatini Renata , Arab Claudia , Abreu Luiz Carlos , Valenti Vitor E. , Monteiro Carlos B. M. , Tannuri Uenis , Tannuri Ana Cristina Aoun
Published: March 2020

Background and Objectives: Cirrhosis is a liver disease that causes about one million deaths annually worldwide. The estimated cirrhosis prevalence ranges from 4.5–9.5% in the general population. Up to 40% of cirrhotic patients are asymptomatic and may be diagnosed late. Studies have described the importance of the functions of the liver and autonomic nervous system (ANS) and their relationship. There is limited information available on non-alcoholic cirrhosis and heart rate variability (HRV), which is a measure of the ANS. This study aimed to evaluate cardiac autonomic modulation through HRV in non-alcoholic cirrhosis individuals reported in previous observational and clinical trial studies. Materials and Methods: We performed a systematic review according to the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) statement using the Medline, Scopus, and Web of Science electronic databases. Five studies were identified and reviewed. Results: HRV was decreased in patients with non-alcoholic cirrhosis, even in the first stage. Conclusions: HRV could be used as a complementary method to improve both the diagnosis and prognosis of non-alcoholic cirrhosis.
The Photoreceptor Components FaWC1 and FaWC2 of Fusarium asiaticum Cooperatively Regulate Light Responses but Play Independent Roles in Virulence Expression
Authors: Tang Ying , Zhu Pinkuan , Lu Zhengyu , Qu Yao , Huang Li , Zheng Ni , Wang Yiwen , Nie Haozhen , Jiang Yina , Xu Ling
Published: March 2020

Fusarium asiaticum belongs to one of the phylogenetical subgroups of the F. graminearum species complex and is epidemically predominant in the East Asia area. The life cycle of F. asiaticum is significantly regulated by light. In this study, the fungal blue light receptor white collar complex (WCC), including FaWC1 and FaWC2, were characterized in F. asiaticum. The knockout mutants ΔFawc1 and ΔFawc2 were generated by replacing the target genes via homologous recombination events. The two mutants showed similar defects in light-induced carotenoid biosynthesis, UV-C resistance, sexual fruiting body development, and the expression of the light-responsive marker genes, while in contrast, all these light responses were characteristics in wild-type (WT) and their complementation strains, indicating that FaWC1 and FaWC2 are involved in the light sensing of F. asiaticum. Unexpectedly, however, the functions of Fawc1 and Fawc2 diverged in regulating virulence, as the ΔFawc1 was avirulent to the tested host plant materials, but ΔFawc2 was equivalent to WT in virulence. Moreover, functional analysis of FaWC1 by partial disruption revealed that its light–oxygen–voltage (LOV) domain was required for light sensing but dispensable for virulence, and its Zinc-finger domain was required for virulence expression but not for light signal transduction. Collectively, these results suggest that the conserved fungal blue light receptor WCC not only endows F. asiaticum with light-sensing ability to achieve adaptation to environment, but it also regulates virulence expression by the individual component FaWC1 in a light-independent manner, and the latter function opens a way for investigating the pathogenicity mechanisms of this important crop disease agent.
Comparative Genomic Analysis Confirms Five Genetic Populations of the Select Agent, Rathayibacter toxicus
Authors: Yasuhara-Bell Jarred , Arif Mohammad , Busot Grethel Y. , Mann Rachel , Rodoni Brendan , Stack James P.
Published: March 2020

Rathayibacter toxicus is a Gram-positive, nematode-vectored bacterium that infects several grass species in the family Poaceae. Unique in its genus, R. toxicus has the smallest genome, possesses a complete CRISPR-Cas system, a vancomycin-resistance cassette, produces tunicamycin, a corynetoxin responsible for livestock deaths in Australia, and is designated a Select Agent in the United States. In-depth, genome-wide analyses performed in this study support the previously designated five genetic populations, with a core genome comprising approximately 80% of the genome for all populations. Results varied as a function of the type of analysis and when using different bioinformatics tools for the same analysis; e.g., some programs failed to identify specific genomic regions that were actually present. The software variance highlights the need to verify bioinformatics results by additional methods; e.g., PCR, mapping genes to genomes, use of multiple algorithms). These analyses suggest the following relationships among populations: RT-IV ↔ RT-I ↔ RT-II ↔ RT-III ↔ RT-V, with RT-IV and RT-V being the most unrelated. This is the most comprehensive analysis of R. toxicus that included populations RT-I and RT-V. Future studies require underrepresented populations and more recent isolates from varied hosts and geographic locations.
Siboglinidae Tubes as an Additional Niche for Microbial Communities in the Gulf of Cádiz—A Microscopical Appraisal
Authors: Rincón-Tomás Blanca , González Francisco Javier , Somoza Luis , Sauter Kathrin , Madureira Pedro , Medialdea Teresa , Carlsson Jens , Reitner Joachim , Hoppert Michael
Published: March 2020

Siboglinids were sampled from four mud volcanoes in the Gulf of Cádiz (El Cid MV, Bonjardim MV, Al Gacel MV, and Anastasya MV). These invertebrates are characteristic to cold seeps and are known to host chemosynthetic endosymbionts in a dedicated trophosome organ. However, little is known about their tube as a potential niche for other microorganisms. Analyses by scanning and transmission electron microscopy showed dense biofilms on the tube in Al Gacel MV and Anastasya MV specimens by prokaryotic cells. Methanotrophic bacteria were the most abundant forming these biofilms as further supported by 16S rRNA sequence analysis. Furthermore, elemental analyses with electron microscopy and energy-dispersive X-ray spectroscopy point to the mineralization and silicification of the tube, most likely induced by the microbial metabolisms. Bacterial and archaeal 16S rRNA sequence libraries revealed abundant microorganisms related to these siboglinid specimens and certain variations in microbial communities among samples. Thus, the tube remarkably increases the microbial biomass related to the worms and provides an additional microbial niche in deep-sea ecosystems.
Optimization of Preservation Methods Allows Deeper Insights into Changes of Raw Milk Microbiota
Authors: Kennang Ouamba Alexandre J. , LaPointe Gisèle , Dufour Simon , Roy Denis
Published: March 2020

The temporal instability of raw milk microbiota drastically affects the reliability of microbiome studies. However, little is known about the microbial integrity in preserved samples. Raw cow milk samples were preserved with azidiol or bronopol and stored at 4 °C, or with dimethyl sulfoxide (DMSO) or a mixture of azidiol and DMSO and stored at −20 °C for up to 30 days. Aliquots of 5-, 10-, and 30-day post-storage were treated with propidium monoazide (PMA), then analyzed by sequencing the 16S rRNA gene V3-V4 and V6-V8 regions. The V6-V8 gave a higher richness and lower diversity than the V3-V4 region. After 5-day storage at 4 °C, the microbiota of unpreserved samples was characterized by a drastic decrease in diversity, and a significant shift in community structure. The treatment with azidiol and DMSO conferred the best community stabilization in preserved raw milk. Interestingly, the azidiol treatment performed as well for up to 10 days, thus appearing as a suitable alternative. However, neither azidiol nor bronopol could minimize fungal proliferation as revealed by PMA-qPCR assays. This study demonstrates the preservative ability of a mixture of azidiol and DMSO and provides deeper insights into the microbial changes occurring during the cold storage of preserved raw milk.
The Complexity of the Holobiont in the Red Sea Coral Euphyllia paradivisa under Heat Stress
Authors: Meron Dalit , Maor-Landaw Keren , Eyal Gal , Elifantz Hila , Banin Ehud , Loya Yossi , Levy Oren
Published: March 2020

The recognition of the microbiota complexity and their role in the evolution of their host is leading to the popularization of the holobiont concept. However, the coral holobiont (host and its microbiota) is still enigmatic and unclear. Here, we explore the complex relations between different holobiont members of a mesophotic coral Euphyllia paradivisa. We subjected two lines of the coral—with photosymbionts, and without photosymbionts (apo-symbiotic)—to increasing temperatures and to antibiotics. The different symbiotic states were characterized using transcriptomics, microbiology and physiology techniques. The bacterial community’s composition is dominated by bacteroidetes, alphaproteobacteria, and gammaproteobacteria, but is dependent upon the symbiont state, colony, temperature treatment, and antibiotic exposure. Overall, the most important parameter determining the response was whether the coral was a symbiont/apo-symbiotic, while the colony and bacterial composition were secondary factors. Enrichment Gene Ontology analysis of coral host’s differentially expressed genes demonstrated the cellular differences between symbiotic and apo-symbiotic samples. Our results demonstrate the significance of each component of the holobiont consortium and imply a coherent link between them, which dramatically impacts the molecular and cellular processes of the coral host, which possibly affect its fitness, particularly under environmental stress.
The Nature and Extent of Plasmid Variation in Chlamydia trachomatis
Authors: Jones Charlotte A. , Hadfield James , Thomson Nicholas R. , Cleary David W. , Marsh Peter , Clarke Ian N. , O’Neill Colette E.
Published: March 2020

Chlamydia trachomatis is an obligate intracellular pathogen of humans, causing both the sexually transmitted infection, chlamydia, and the most common cause of infectious blindness, trachoma. The majority of sequenced C. trachomatis clinical isolates carry a 7.5-Kb plasmid, and it is becoming increasingly evident that this is a key determinant of pathogenicity. The discovery of the Swedish New Variant and the more recent Finnish variant highlight the importance of understanding the natural extent of variation in the plasmid. In this study we analysed 524 plasmid sequences from publicly available whole-genome sequence data. Single nucleotide polymorphisms (SNP) in each of the eight coding sequences (CDS) were identified and analysed. There were 224 base positions out of a total 7550 bp that carried a SNP, which equates to a SNP rate of 2.97%, nearly three times what was previously calculated. After normalising for CDS size, CDS8 had the highest SNP rate at 3.97% (i.e., number of SNPs per total number of nucleotides), whilst CDS6 had the lowest at 1.94%. CDS5 had the highest total number of SNPs across the 524 sequences analysed (2267 SNPs), whereas CDS6 had the least SNPs with only 85 SNPs. Calculation of the genetic distances identified CDS6 as the least variable gene at the nucleotide level (d = 0.001), and CDS5 as the most variable (d = 0.007); however, at the amino acid level CDS2 was the least variable (d = 0.001), whilst CDS5 remained the most variable (d = 0.013). This study describes the largest in-depth analysis of the C. trachomatis plasmid to date, through the analysis of plasmid sequence data mined from whole genome sequences spanning 50 years and from a worldwide distribution, providing insights into the nature and extent of existing variation within the plasmid as well as guidance for the design of future diagnostic assays. This is crucial at a time when single-target diagnostic assays are failing to detect natural mutants, putting those infected at risk of a serious long-term and life-changing illness.
Detection of Wood Mice (Apodemus sylvaticus) Carrying Non-Tuberculous Mycobacteria Able to Infect Cattle and Interfere with the Diagnosis of Bovine Tuberculosis
Authors: Varela-Castro Lucía , Torrontegi Olalla , Sevilla Iker A. , Barral Marta
Published: March 2020

Mycobacterial infections caused by the Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacteria (NTM) are of great medical and veterinary relevance. The aim of this research was to study whether small mammals play a role in the epidemiology of mycobacterioses. Four samplings of 100 traps were performed in each of three cattle farms with previous history of tuberculosis or NTM between 2017 and 2018. A total of 108 animals belonging to seven species were trapped, classified, and necropsied, and tissues were submitted to microbiological and molecular methods for mycobacteria identification. The wood mouse (Apodemus sylvaticus) was the most abundant species (87%). No MTC was detected but six different NTM were identified (M. intracellulare, M. avium subsp. paratuberculosis, M. gordonae, M. celatum, M. fortuitum, and a not determined Mycobacterium sp.), showing a prevalence of 6.5%. No significant association was found between mycobacteria prevalence and the analyzed factors. Although a role in the epidemiology of MTC could not be attributed to small mammals, A. sylvaticus carries NTM that could be pathogenic or interfere with the diagnosis of tuberculosis. According to our results, there is a risk of NTM transmission at the wildlife–livestock interface through potential indirect contacts between small mammals and cattle.
Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018)
Authors: Wareth Gamal , Brandt Christian , Sprague Lisa D. , Neubauer Heinrich , Pletz Mathias W.
Published: March 2020

Acinetobacter (A.) baumannii has gained global notoriety as a significant nosocomial pathogen because it is frequently associated with multi-drug resistance and hospital-based outbreaks. There is a substantial difference in the incidence of A. baumannii infections between different countries and within Germany. However, its continuous spread within Germany is a matter of concern. A systematic literature search and analysis of the literature published between 2000 and 2018 on A. baumannii in humans was performed. Forty-four studies out of 216 articles met the criteria for inclusion, and were selected and reviewed. The number of published articles is increasing over time gradually. Case reports and outbreak investigations are representing the main body of publications. North Rhine-Westphalia, Hesse and Baden-Wuerttemberg were states with frequent reports. Hospitals in Cologne and Frankfurt were often mentioned as specialized institutions. Multiresistant strains carrying diverse resistance genes were isolated in 13 of the 16 German states. The oxacillinase blaOXA-23-like, intrinsic blaOXA-51-like, blaOXA-58 variant, blaNDM-1, blaGES-11, blaCTX-M and blaTEM are the most predominant resistance traits found in German A. baumannii isolates. Five clonal lineages IC-2, IC-7, IC-1, IC-4 and IC-6 and six sequence types ST22, ST53, ST195, ST218, ST944/ST78 and ST348/ST2 have been reported. Due to multidrug resistance, colistin, tigecycline, aminoglycosides, fosfomycin, ceftazidime/avibactam and ceftolozan/tazobactam were often reported to be the only effective antibiotics left to treat quadruple multi-resistant Gram-negative (4MRGN) A. baumannii. Dissemination and infection rates of A. baumannii are on the rise nationwide. Hence, several aspects of resistance development and pathogenesis are not fully understood yet. Increased awareness, extensive study of mechanisms of resistance and development of alternative strategies for treatment are required. One-Health genomic surveillance is needed to understand the dynamics of spread, to identify the main reservoirs and routes of transmission and to develop targeted intervention strategies.
The Effect of DHA Supplementation on Cognition in Patients with Bipolar Disorder: An Exploratory Randomized Control Trial
Authors: Ciappolino Valentina , DelVecchio Giuseppe , Prunas Cecilia , Andreella Angela , Finos Livio , Caletti Elisabetta , Siri Francesca , Mazzocchi Alessandra , Botturi Andrea , Turolo Stefano , Agostoni Carlo , Brambilla Paolo
Published: March 2020

Bipolar disorder (BD) is a severe mental disorder with a wide range of cognitive deficits, both in the euthymic and acute phase of the disease. Interestingly, in recent years, there has been a growing interest in investigating the impact of ω-3 polyunsaturated fatty acids on cognition in BD. In this context, the aim of this study is to evaluate the effect of docosahexaenoic acid (C22:6 ω-3, DHA) supplementation on cognitive performances in euthymic BD patients. This is an exploratory, single-centre, double-blind randomized controlled trial evaluating 12 weeks DHA supplementation (1250 mg daily) vs. a placebo (corn oil) in 31 euthymic BD patients compared to 15 healthy controls (HCs) on cognitive functions, assessed by the Brief Assessment of Cognition in Affective Disorder (BAC-A). Plasma levels of DHA were measured. After 12 weeks of treatment, no significant group differences were observed in all neuropsychological tests between the four groups, except for the emotion inhibition test, where HCs with DHA had higher scores compared to either BD with DHA (z = 3.9, p = 0.003) or BD with placebo (t = 3.7, p = 0.005). Although our results showed that DHA could be effective for ameliorating cognition in healthy subjects, future studies are still needed to clarify the impact of DHA on cognition in BD.
Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag
Authors: Tetsuo Madoka , Matsuno Keita , Tamura Tomokazu , Fukuhara Takasuke , Kim Taksoo , Okamatsu Masatoshi , Tautz Norbert , Matsuura Yoshiharu , Sakoda Yoshihiro
Published: March 2020

A serum neutralization test (SNT) is an essential method for the serological diagnosis of pestivirus infections, including classical swine fever, because of the cross reactivity of antibodies against pestiviruses and the non-quantitative properties of antibodies in an enzyme-linked immunosorbent assay. In conventional SNTs, an immunoperoxidase assay or observation of cytopathic effect after incubation for 3 to 7 days is needed to determine the SNT titer, which requires labor-intensive or time-consuming procedures. Therefore, a new SNT, based on the luciferase system and using classical swine fever virus, bovine viral diarrhea virus, and border disease virus possessing the 11-amino-acid subunit derived from NanoLuc luciferase was developed and evaluated; this approach enabled the rapid and easy determination of the SNT titer using a luminometer. In the new method, SNT titers can be determined tentatively at 2 days post-infection (dpi) and are comparable to those obtained by conventional SNTs at 3 or 4 dpi. In conclusion, the luciferase-based SNT can replace conventional SNTs as a high-throughput antibody test for pestivirus infections.
Cerebral HSV-1 Vasculitis as a Fatal Complication of Immunosuppression in Non-Hodgkin´s Lymphoma: A Case Report and Review of the Literature
Authors: Nardone Raffaele , Carnicelli Luca , Brigo Francesco , Pikija Slaven , Hauer Larissa , Sellner Johann
Published: March 2020

Patients with lymphoma are predisposed to infection because of the immunocompromised state related to the disease itself and as a consequence of chemo-/radiotherapy. Here, we report a case of Herpes-simplex virus encephalitis (HSE) in an immunosuppressed patient with splenic marginal zone lymphoma (SMZL), a rare indolent variant of non-Hodgkin´s lymphoma (NHL). The course was complicated febrile neutropenia and HSV-1-related cerebral vasculitis causing progressive ischemic stroke. This case illustrates the expanding spectrum of atypical clinical and radiological manifestations of HSE in patients treated with myelotoxic drugs. Moreover, we summarize the few central nervous system manifestations of SMZL reported in the literature and discuss distinct causes of neurological deterioration in patients with NHL.
Evaluation of Quality Control Methods for Foot-And-Mouth Disease Vaccines by High-Performance Liquid Chromatography
Authors: Kim Mun-Hyeon , Yun Seon-Jong , Kim Yeon-Hee , Lee Hyang-Sim , Kim Ji-Yeon , Kim Ji-Ye , Kang JeongWoo , Kim Yong-Sang , Seo Min-Goo
Published: March 2020

Foot-and-mouth disease (FMD) is considered one of the highly contagious viral infections affecting livestock. In Korea, an FMD vaccination policy has been implemented nationwide since 2010 for the prevention and control of FMD. Since the vaccines are imported from various countries, standardized quality control measures are critical. In this study, we aimed to validate a high-performance liquid chromatography (HPLC) device in the Animal and Plant Quarantine Agency lab and identify an appropriate FMD vaccine pretreatment method for HPLC—a simple, reliable, and practical method to measure antigen content. Based on the analyses of specificity, linearity, accuracy, repeatability, intermediate precision, limits of detection, and limits of quantification using FMD standard samples, we validated the method using a standard material. Overall, we confirmed that the HPLC technique is effective for the quantitative assessment of the FMD virus 146S antigen in Korea. Using commercial FMD vaccines, we evaluated three separation methods and identified the method using n-pentanol and trichloroethylene as optimal for HPLC analysis. Our HPLC method was effective for the analytical detection of the antigen content in FMD vaccine, and it may be useful as a reference method for national lot-release testing.
Lineage, Antimicrobial Resistance and Virulence of Citrobacter spp
Authors: Liu Liyun , Qin Liyun , Hao Shuai , Lan Ruiting , Xu Baohong , Guo Yumei , Jiang Ruiping , Sun Hui , Chen Xiaoping , LV Xinchao , Xu Jianguo , Zhao Chuan
Published: March 2020

Citrobacter spp. are opportunistic human pathogens which can cause nosocomial infections, sporadic infections and outbreaks. In order to determine the genetic diversity, in vitro virulence properties and antimicrobial resistance profiles of Citrobacter spp., 128 Citrobacter isolates obtained from human diarrheal patients, foods and environment were assessed by multilocus sequence typing (MLST), antimicrobial susceptibility testing and adhesion and cytotoxicity testing to HEp-2 cells. The 128 Citrobacter isolates were typed into 123 sequence types (STs) of which 101 were novel STs, and these STs were divided into five lineages. Lineages I and II contained C. freundii isolates; Lineage III contained all C. braakii isolates, while Lineage IV and V contained C. youngae isolates. Lineages II and V contained more adhesive and cytotoxic isolates than Lineages I, III, and IV. Fifty-one of the 128 isolates were found to be multidrug-resistant (MDR, ≥3) and mainly distributed in Lineages I, II, and III. The prevalence of quinolone resistance varied with Lineage III (C. braakii) having the highest proportion of resistant isolates (52.6%), followed by Lineage I (C. freundii) with 23.7%. Seven qnrB variants, including two new alleles (qnrB93 and qnrB94) were found with Lineage I being the main reservoir. In summary, highly cytotoxic MDR isolates from diarrheal patients may increase the risk of severe disease.
Animal Models of Lassa Fever
Authors: Sattler Rachel A. , Paessler Slobodan , Ly Hinh , Huang Cheng
Published: March 2020

Lassa virus (LASV), the causative agent of Lassa fever, is estimated to be responsible for up to 300,000 new infections and 5000 deaths each year across Western Africa. The most recent 2018 and 2019 Nigerian outbreaks featured alarmingly high fatality rates of up to 25.4%. In addition to the severity and high fatality of the disease, a significant population of survivors suffer from long-term sequelae, such as sensorineural hearing loss, resulting in a huge socioeconomic burden in endemic regions. There are no Food and Drug Administration (FDA)-approved vaccines, and therapeutics remain extremely limited for Lassa fever. Development of countermeasures depends on relevant animal models that can develop a disease strongly mimicking the pathogenic features of Lassa fever in humans. The objective of this review is to evaluate the currently available animal models for LASV infection with an emphasis on their pathogenic and histologic characteristics as well as recent advances in the development of a suitable rodent model. This information may facilitate the development of an improved animal model for understanding disease pathogenesis of Lassa fever and for vaccine or antiviral testing.
Medicinal Plants and Their Traditional Uses in Local Communities around Cherangani Hills, Western Kenya
Authors: Mbuni Yuvenalis M. , Wang Shengwei , Mwangi Brian N. , Mbari Ndungu J. , Musili Paul M. , Walter Nyamolo O. , Hu Guangwan , Zhou Yadong , Wang Qingfeng
Published: March 2020

Medicinal plants are vital sources of easily accessible remedy used in the countryside healthcare system. This study aimed to find and make record of plants that are used for medicinal therapy by three communities living in Cherangani Hills. So far no single study has documented medicinal plants as a whole in the area. Ethnobotanical data were obtained through interviewing informants using semi-structured questionnaires and extracting information from journals and books. Descriptive statistical analysis was applied to describe the data. Overall 296 plant species from 80 families and 191 genera were identified. Asteraceae family was the most dominant, representing 10.7% of the total plant species recorded. Roots (35.9%) represented the most commonly used parts of the plant. The commonly used method of preparation was decoction (54.9%). The reported diseases were classified into 14 diverse ailment groups out of the 81 health conditions on their underlying user reports. Rural communities in Cherangani Hills are rich sources of plants with medicinal properties. Therapeutic uses of the compiled plants provide basic information that can aid scientists to conduct additional research dedicated to conservation of species and pharmacological studies of species with the greatest significance.
Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of Actinidia eriantha ‘Ganmi 6’
Authors: Jiang Zhiqiang , Huang Qing , Jia Dongfeng , Zhong Min , Tao Junjie , Liao Guanglian , Huang Chunhui , Xu Xiaobiao
Published: March 2020

Studies on organic acid metabolism have been mainly concentrated on the fruit, whereas, few have focused on the mechanism of high organic acids content in the fruit of Actinidia eriantha. Fruits of ‘Ganmi 6’ harvested at eleven developmental periods were used as materials. The components and content of organic acids were determined by high-performance liquid chromatography (HPLC) system, the activities of the related enzyme were detected, and gene expression levels were measured by quantitative real-time PCR (qRT-PCR). Components of ascorbic acid (AsA) and eight kinds of organic acids were detected. These results showed that quinic acid and citric acid were the main organic acids in the fruit of ‘Ganmi 6’. Correlation analysis showed that NADP-Quinate dehydrogenase (NADP-QDH), NADP-Shikimate dehydrogenase (NADP-SDH), and Cyt-Aconitase (Cyt-Aco) may be involved in regulating organic acids biosynthesis. Meanwhile, the SDH gene may play an important role in regulating the accumulation of citric acid. In this study, the activities of NADP-SDH, Mit-Aconitase (Mit-Aco), and NAD-Isocitrate dehydrogenase (NAD-IDH) were regulated by their corresponding genes at the transcriptional level. The activity of Citrate synthase (CS) may be affected by post-translational modification. Our results provided new insight into the characteristics of organic acid metabolism in the fruit of A. eriantha.
Seed Biology of Lepidium apetalum (Brassicaceae), with Particular Reference to Dormancy and Mucilage Development
Authors: Zhang Keliang , Zhang Yin , Ji Yusong , Walck Jeffrey L. , Tao Jun
Published: March 2020

Lepidium apetalum (Brassicaceae) is an annual or biennial weed widely distributed in Asia and Europe. The outer surface of L. apetalum seeds produces a large amount of mucilage. The primary aim of this study was to explore the dormancy characteristics and to determine how mucilage develops. The role of mucilage in water absorption/dehydration, the effects of after-ripening, gibberellin acid (GA3), cold stratification and seed coat scarification on germination, the role of mucilage in germination and seedling growth during drought, and the progress of mucilage production during seed development were investigated. The results indicate that the best temperature regime for germination was 10/20 °C. After-ripening, GA3 and seed coat scarification helped to break dormancy. Light promoted germination. Seedling growth of mucilaged seeds were significantly higher than those of demucilaged seeds at −0.606 and −1.027 MPa. Anatomical changes during seed development showed that mucilage was derived from the outer layer of the outer integument cells. Our findings suggest that seeds of L. apetalum exhibited non-deep physiological dormancy. The dormancy characteristics along with mucilage production give seeds of L. apetalum a competitive advantage over other species, and thus contribute to its potential as a weed. Effective control of this weed can be achieved by deep tillage.