bluuebunny/crossref_metadata_2025 huggingface dataset:
Epistasis in neurotransmitter receptors linked to posttraumatic stress disorder and major depressive disorder comorbidity in traumatized Chinese
Authors: Xu Ling , Zhang Jingyi , Yang Haibo , Cao Chengqi , Fang Ruojiao , Liu Ping , Luo Shu , Wang Binbin , Zhang Kunlin , Wang Li
Published: February 2024

Background

Posttraumatic stress disorder (PTSD) and major depressive disorder (MDD) comorbidity occurs through exposure to trauma with genetic susceptibility. Neuropeptide-Y (NPY) and dopamine are neurotransmitters associated with anxiety and stress-related psychiatry through receptors. We attempted to explore the genetic association between two neurotransmitter receptor systems and the PTSD–MDD comorbidity.

Methods

Four groups were identified using latent profile analysis (LPA) to examine the patterns of PTSD and MDD comorbidity among survivors exposed to earthquake-related trauma: low symptoms, predominantly depression, predominantly PTSD, and PTSD–MDD comorbidity. NPY2R (rs4425326), NPY5R (rs11724320), DRD2 (rs1079597), and DRD3 (rs6280) were genotyped from 1,140 Chinese participants exposed to earthquake-related trauma. Main, gene–environment interaction (G × E), and gene–gene interaction (G × G) effects for low symptoms, predominantly depression, and predominantly PTSD were tested using a multinomial logistic model with PTSD–MDD comorbidity as a reference.

Results

The results demonstrated that compared to PTSD–MDD comorbidity, epistasis (G × G) NPY2R-DRD2 (rs4425326 × rs1079597) affects low symptoms (β = −0.66, OR = 0.52 [95% CI: 0.32–0.84], p = 0.008, pperm = 0.008) and predominantly PTSD (β = −0.56, OR = 0.57 [95% CI: 0.34–0.97], p = 0.037, pperm = 0.039), while NPY2R-DRD3 (rs4425326 × rs6280) impacts low symptoms (β = 0.82, OR = 2.27 [95% CI: 1.26–4.10], p = 0.006, pperm = 0.005) and predominantly depression (β = 1.08, R = 2.95 [95% CI: 1.55–5.62], p = 0.001, pperm = 0.001). The two G × G effects are independent.

Conclusion

NPY and dopamine receptor genes are related to the genetic etiology of PTSD–MDD comorbidity, whose specific mechanisms can be studied at multiple levels.
Dietary pyrroloquinoline quinone hinders aging progression in male mice and D-galactose-induced cells
Authors: Mohamad Ishak Nur Syafiqah , Kikuchi Midori , Ikemoto Kazuto
Published: February 2024

Background: Understanding and promoting healthy aging has become a necessity in the modern world, where life expectancy is rising. The prospective benefits of the antioxidant pyrroloquinoline quinone (PQQ) in healthy aging are promising. However, its role in aging remains unclear. Thus, this study aimed to investigate the effect of PQQ on preventing the progression of aging and to explore its underlying molecular mechanisms.

Methods: Naturally aged C57BL/6J male mice were fed a normal diet with or without PQQ (20 mg/kg/day) for 10 weeks. Body composition was measured by bioimpedance at weeks 0 and 8. The integument conditions were evaluated at weeks 0, 4, and 8. Muscle strength and function were examined at week 8. At the ninth week, computed tomography images of the mice were captured, and blood and tissue samples were collected. The levels of inflammatory cytokines in the gastrocnemius muscle were measured, and the muscle fiber cross-sectional area in the soleus muscle was examined. Additionally, a D-galactose (D-gal)-induced cell aging model was used to study the effects of PQQ intervention on cell proliferation, senescence, differentiation, ROS levels, and mitochondrial function in myoblasts (C2C12). Cell proliferation and monolayer permeability of D-gal-induced intestinal epithelial cells (IEC6) were also examined.

Results: Aged mice suffered from malnutrition; however, PQQ supplementation ameliorated this effect, possibly by improving metabolic dysfunction and small intestinal performance. PQQ prevented rapid loss of body fat and body fluid accumulation, attenuated muscle atrophy and weakening, reduced chronic inflammation in skeletal muscles, and improved skin and coating conditions in aged mice. Furthermore, PQQ intervention in D-gal-treated C2C12 cells improved mitochondrial function, reduced cellular reactive oxygen species (ROS) levels and senescence, and enhanced cell differentiation, consequently preventing age-related muscle atrophy. In addition, PQQ increased cell proliferation in D-gal-treated IEC6 cells and consequently improved intestinal barrier function.

Conclusion: PQQ could hinder the aging process and particularly attenuate muscle atrophy, and muscle weakness by improving mitochondrial function, leading to reduced age-related oxidative stress and inflammation in muscles. PQQ may also ameliorate malnutrition caused by intestinal barrier dysfunction by enhancing IEC proliferation. This study provides evidence for the role of PQQ in aging and suggests that PQQ may be a potential nutritional supplementation that can be included in healthy aging strategies.
Impact of the COVID-19 pandemic and COVID vaccination campaign on imaging case volumes and medicolegal aspects
Authors: Battaglia Caterina , Manti Francesco , Mazzuca Daniela , Cutruzzolà Antonio , Corte Marcello Della , Caputo Fiorella , Gratteri Santo , Laganà Domenico
Published: February 2024

Purpose

The coronavirus pandemic (COVID-19) significantly impacted the global economy and health. Italy was one of the first and most affected countries. The objective of our study was to assess the impact of the pandemic and the vaccination campaign on the radiological examinations performed in a radiology department of a tertiary center in Southern Italy.

Materials and methods

We analyzed weekly and retrospectively electronic medical records of case volumes performed at the Radiology Department of “Mater Domini” University Hospital of Catanzaro from March 2020 to March 2022, comparing them with the volumes in the same period of the year 2019. We considered the origin of patients (outpatient, inpatient) and the type of examinations carried out (x-ray, mammography, CT, MRI, and ultrasound). A non-parametric test (Wilcoxon Signed Rank test) was applied to evaluate the average volumes.

Results

Total flows in the pandemic period from COVID-19 were lower than in the same pre-pandemic period with values of 552 (120) vs. 427 (149) median (IQR) (p < 0.001). The vaccination campaign allowed the resumption of the pre-vaccination pandemic with total flows 563 (113) vs. 427 (149) median (IQR) p < 0.001. In the post-vaccination period, the number of examinations was found to overlap with the pre-COVID period.

Conclusion

The pandemic impacted the volume of radiological examinations performed, particularly with the reduction of tests in outpatients. The vaccination allowed the return to the pre-COVID period imaging case volumes.
Continuous inline monitoring of glucose in an organ-on-chip using FreeStyle™ libre glucometers
Authors: Sánchez-Salazar Mónica Gabriela , Garza-Garza Regina , Crespo-López Oliver Regina , Jerezano-Flores Valeri Sofía , Gallegos-Martínez Salvador , Ramos-Meizoso Sofía , Verduzco-Valenzuela Manuel , Trujillo-de Santiago Grissel , Alvarez Mario Moisés
Published: February 2024

Introduction: Organ-on-chips have become an effective platform for studying the physiology of tissues and organs and for evaluating the safety and efficacy of drugs. In these systems, the inline monitoring of key parameters of biological performance (i.e., glucose, oxygen, or lactic acid concentrations) provides valuable information regarding the cell/tissue physiological state. However, significant limitations still exist when attempting to obtain inline information in these systems, and the microsensing technology of on-chip measurement of key parameters is still limited by size, cost, and availability.

Methods: Here, we demonstrate the use of a commercially available glucometer (FreestleTM Libre; Abbott), normally used for continuous determination of blood glucose levels, to provide continuous inline measurements of the glucose concentration in tumor-on-chips. Here, we employed a colorectal tumor-on-chip as a first demonstration model and measured the on-chip concentration of glucose continuously for extended culture times (2 weeks).

Results and Discussion: We show that inline glucose readings are reproducible and enable the accurate determination of glucose consumption rates (GCRs) by a tumor cell culture. In turn, the GCR measurements provide valuable information regarding the changes in the metabolic activity of the on-chip cultures following inlet perturbations (i.e., delivery of pulses of glucose, culture media additives, and drugs). Inline continuous glucose sensors will be useful tools in organ-on-chip research and will greatly enable cancer research in tumor-on-chip systems.
High Aspect Ratio Composite Wings: Geometrically Nonlinear Aeroelasticity, Multi-Disciplinary Design Optimization, Manufacturing, and Experimental Testing
Authors: Farsadi Touraj , Ahmadi Majid , Sahin Melin , Haddad Khodaparast Hamed , Kayran Altan , Friswell Michael I.
Published: February 2024

In the field of aerospace engineering, the design and manufacturing of high aspect ratio composite wings has become a focal point of innovation and efficiency. These long, slender wings, constructed with advanced materials such as carbon fiber and employing efficient manufacturing methods such as vacuum bagging, hold the promise of significantly lighter aircraft, reduced fuel consumption, and enhanced overall performance. However, to fully realize these benefits, it is imperative to address a multitude of structural and aeroelastic constraints. This research presents a novel aeroelastically tailored Multi-objective, Multi-disciplinary Design Optimization (MMDO) approach that seamlessly integrates numerical optimization techniques to minimize weight and ensure structural integrity. The optimized wing configuration is then manufactured, and a Ground Vibration Test (GVT) and static deflection analysis using the Digital Image Correlation (DIC) system are used to validate and correlate with the numerical model. Within the fully automated in-house Nonlinear Aeroelastic Simulation Software (NAS2) package (version v1.0), the integration of analytical tools offers a robust numerical approach for enhancing aeroelastic and structural performance in the design of composite wings. Nonlinear aeroelastic analyses and tailoring are included, and a population-based stochastic optimization is used to determine the optimum design within NAS2. These analytical tools contribute to a comprehensive and efficient methodology for designing composite wings with improved aeroelastic and structural characteristics. This comprehensive methodology aims to produce composite wings that not only meet rigorous safety and performance standards but also drive cost-efficiency in the aerospace industry. Through this multidisciplinary approach, the authors seek to underscore the pivotal role of tailoring aeroelastic solutions in the advanced design and manufacturing of high aspect ratio composite wings, thereby contributing to the continued evolution of aerospace technology.
Modelling and Simulation in High-Fidelity Crash Analysis of NGCTR-TD Composite Wing
Authors: Di Palma Luigi , Nardone Mariacristina , Pezzella Claudio , Belardo Marika
Published: February 2024

This paper presents a methodology that involves the development of high-fidelity modeling and simulation procedures aimed at supporting virtual certification for crashworthiness requirements specific to tiltrotor aircraft, addressing the critical need for accurate safety requirement fulfillment predictions and weight containment of wing. The unique crashworthiness requirement for tiltrotor wings necessitates a design that can ensure a controlled failure during survivable crash events. This is to alleviate the inertial load acting on the fuselage, thereby protecting occupants from injuries and fire while ensuring the integrity of escape paths. The objective of this methodology is to simulate the crash effects on the entire wing using explicit, non-linear, and time-dependent FE analysis. This approach verifies the spanwise placement of the frangible sections, the mode of failure, the loads acting on the fuselage links, and the acceleration transmitted to the structure. This study focuses on a standalone analysis.
Current Understanding of Equine Gut Dysbiosis and Microbiota Manipulation Techniques: Comparison with Current Knowledge in Other Species
Authors: Boucher Laurie , Leduc Laurence , Leclère Mathilde , Costa Marcio Carvalho
Published: February 2024

Understanding the importance of intestinal microbiota in horses and the factors influencing its composition have been the focus of many studies over the past few years. Factors such as age, diet, antibiotic administration, and geographic location can affect the gut microbiota. The intra- and inter-individual variability of fecal microbiota in horses complicates its interpretation and has hindered the establishment of a clear definition for dysbiosis. Although a definitive causal relationship between gut dysbiosis in horses and diseases has not been clearly identified, recent research suggests that dysbiosis may play a role in the pathogenesis of various conditions, such as colitis and asthma. Prebiotics, probiotics, and fecal microbiota transplantation to modulate the horse’s gastrointestinal tract may eventually be considered a valuable tool for preventing or treating diseases, such as antibiotic-induced colitis. This article aims to summarize the current knowledge on the importance of intestinal microbiota in horses and factors influencing its composition, and also to review the published literature on methods for detecting dysbiosis while discussing the efficacy of gut microbiota manipulation in horses.
Influence of Frictional Stress Models on Simulation Results of High-Pressure Dense-Phase Pneumatic Conveying in Horizontal Pipe
Authors: Ding Shengxian , Zhou Haijun , Tang Wenying , Xiao Ruien , Zhou Jiaqi
Published: February 2024

Based on the two-fluid model, a three-zone drag model was developed, and the kinetic theory of granular flows and the Schneiderbauer solids wall boundary model were modified to establish a new three-dimensional (3D) unsteady mathematical model for high-pressure dense-phase pneumatic conveying in horizontal pipe. With this mathematical model, the influence of the three frictional stress models, namely Dartevelle frictional stress model, Srivastava and Sundaresan frictional stress model, and the modified Berzi frictional stress model, on the simulation result was explored. The simulation results showed that the three frictional stress models accurately predicted the pressure drop and its variations with supplementary gas in the horizontal pipe, with relative errors ranging from −4.91% to +7.60%. Moreover, the predicted solids volume fraction distribution in the cross-section of the horizontal pipe using these frictional stress models exhibited good agreement with the electrical capacitance tomography (ECT) images. Notably, the influence of the three frictional stress models on the simulation results was predominantly observed in the transition region and deposited region. In the deposited region, stronger frictional stress resulting in lower solids volume fraction and a higher pressure drop in the horizontal pipe were observed. Among the three frictional stress models, the simulation results with the modified Berzi frictional stress model aligned better with the experimental data. Therefore, the modified Berzi frictional stress model is deemed more suitable for simulating high-pressure dense-phase pneumatic conveying in horizontal pipe.
Managing Vascular Pedicle Exposure in Free Tissue Transfer Using a Reprocessed Micronized Dermal Substitute in Lower Extremity Reconstructions
Authors: Kim Daheui , Lee Jun Hyeok , Park Min Suk , Ahn Ma Rhip , Jun Daiwon , Lee Jung Ho
Published: February 2024

Lower extremity reconstruction is challenging because of its intricate anatomy and dynamic biomechanics. Although microsurgical free tissue transfer offers pivotal solutions to limited local tissue availability, vascular pedicle exposure after free tissue transfer is common. We evaluated a novel method of managing pedicle exposure after free tissue transfer using a reprocessed micronized dermal substitute. Ten patients who underwent lower-extremity reconstruction using free tissue transfer and micronized dermal substitute between January and December 2023 were retrospectively reviewed. When native tissue could not be closed over the pedicle, reprocessed micronized artificial dermal matrix (rmADM) was cut and stacked to protect and stabilize it. Epithelialization was achieved by secondary skin grafting or healing by secondary intention. Flap dimensions, recipient artery and vein, ADM size, time required for granulation tissue maturation and complete epithelialization, and flap outcomes were analyzed. The mean age was 55.80 ± 20.70 years, and six patients (60%) were diabetic. The mean rmADM coverage area was 8.70 ± 8.41 cm2, and the average time required for complete epithelialization was 50.89 ± 14.21 days. Except for one total necrosis due to bypass graft failure, nine limbs were successfully salvaged. Application of rmADM offers numerous advantages, including vascular collapse prevention, moisture maintenance, granulation tissue growth promotion, and pedicle stabilization.
Multiscale Mechanical Characterization of Polyether-2-ketone (PEKK) for Biomedical Application
Authors: Serino Gianpaolo , Distefano Fabio , Zanetti Elisabetta M. , Pascoletti Giulia , Epasto Gabriella
Published: February 2024

Polyether-ether-2-ketone (PEKK) is a high-performance thermoplastic polymer used in various fields, from aerospace to medical applications, due to its exceptional mechanical and thermal properties. Nonetheless, the mechanical behavior of 3D-printed PEKK still deserves to be more thoroughly investigated, especially in view of its production by 3D printing, where mechanical properties measured at different scales are likely to be correlated to one another and to all play a major role in determining biomechanical properties, which include mechanical strength on one side and osteointegration ability on the other side. This work explores the mechanical behavior of 3D-printed PEKK through a multiscale approach, having performed both nanoindentation tests and standard tensile and compression tests, where a detailed view of strain distribution was achieved through Digital Image Correlation (DIC) techniques. Furthermore, for specimens tested up to failure, their fractured surfaces were analyzed through Scanning Electron Microscopy (SEM) to clearly outline fracture modes. Additionally, the internal structure of 3D-printed PEKK was explored through Computed Tomography (CT) imaging, providing a three-dimensional view of the internal structure and the presence of voids and other imperfections. Finally, surface morphology was analyzed through confocal microscopy. The multiscale approach adopted in the present work offers information about the global and local behavior of the PEKK, also assessing its material properties down to the nanoscale. Due to its novelty as a polymeric material, no previous studies have approached a multiscale analysis of 3D-printed PEKK. The findings of this study contribute to a comprehensive understanding of 3D-printed PEKK along with criteria for process optimization in order to customize its properties to meet specific application requirements. This research not only advances the knowledge of PEKK as a 3D-printing material but also provides insights into the multifaceted nature of multiscale material characterization.
The Mechanisms Underlying the Beneficial Impact of Aerobic Training on Cancer-Related Fatigue: A Conceptual Review
Authors: Fontvieille Adeline , Parent-Roberge Hugo , Fülöp Tamás , Pavic Michel , Riesco Eléonor
Published: February 2024

Cancer-related fatigue (CRF) is a prevalent and persistent issue affecting cancer patients, with a broad impact on their quality of life even years after treatment completion. The precise mechanisms underlying CRF remain elusive, yet its multifaceted nature involves emotional, physical, and cognitive dimensions. The absence of effective medical treatments has prompted researchers to explore integrative models for potential insights. Notably, physical exercise emerges as a promising strategy for managing CRF and related symptoms, as studies showed a reduction in CRF ranging from 19% to 40%. Current recommendations highlight aerobic training at moderate intensity as beneficial, although questions about a dose–response relationship and the importance of exercise intensity persist. Despite the positive impact of exercise on CRF, the underlying mechanisms remain elusive. This review aims to provide a theoretical model explaining how aerobic exercise may alleviate CRF. Focusing on acute exercise effects, this review delves into the potential influence on peripheral and neural inflammation, immune function dysregulation, and neuroendocrine system disruptions. The objective is to enhance our understanding of the intricate relationship between exercise and CRF, ultimately paving the way for tailored interventions and potential pharmacological treatments for individuals unable to engage in physical exercise.
Tumor Response Evaluation Using iRECIST: Feasibility and Reliability of Manual Versus Software-Assisted Assessments
Authors: Ristow Inka , Well Lennart , Wiese Nis Jesper , Warncke Malte , Tintelnot Joseph , Karimzadeh Amir , Koehler Daniel , Adam Gerhard , Bannas Peter , Sauer Markus
Published: February 2024

Objectives: To compare the feasibility and reliability of manual versus software-assisted assessments of computed tomography scans according to iRECIST in patients undergoing immune-based cancer treatment. Methods: Computed tomography scans of 30 tumor patients undergoing cancer treatment were evaluated by four independent radiologists at baseline (BL) and two follow-ups (FU), resulting in a total of 360 tumor assessments (120 each at BL/FU1/FU2). After image interpretation, tumor burden and response status were either calculated manually or semi-automatically as defined by software, respectively. The reading time, calculated sum of longest diameter (SLD), and tumor response (e.g., “iStable Disease”) were determined for each assessment. After complete data collection, a consensus reading among the four readers was performed to establish a reference standard for the correct response assignments. The reading times, error rates, and inter-reader agreement on SLDs were statistically compared between the manual versus software-assisted approaches. Results: The reading time was significantly longer for the manual versus software-assisted assessments at both follow-ups (median [interquartile range] FU1: 4.00 min [2.17 min] vs. 2.50 min [1.00 min]; FU2: 3.75 min [1.88 min] vs. 2.00 min [1.50 min]; both p < 0.001). Regarding reliability, 2.5% of all the response assessments were incorrect at FU1 (3.3% manual; 0% software-assisted), which increased to 5.8% at FU2 (10% manual; 1.7% software-assisted), demonstrating higher error rates for manual readings. Quantitative SLD inter-reader agreement was inferior for the manual compared to the software-assisted assessments at both FUs (FU1: ICC = 0.91 vs. 0.93; FU2: ICC = 0.75 vs. 0.86). Conclusions: Software-assisted assessments may facilitate the iRECIST response evaluation of cancer patients in clinical routine by decreasing the reading time and reducing response misclassifications.
Frontline and Relapsed Rhabdomyosarcoma (FaR-RMS) Clinical Trial: A Report from the European Paediatric Soft Tissue Sarcoma Study Group (EpSSG)
Authors: Chisholm Julia , Mandeville Henry , Adams Madeleine , Minard-Collin Veronique , Rogers Timothy , Kelsey Anna , Shipley Janet , van Rijn Rick , de Vries Isabelle , van Ewijk Roelof , de Keizer Bart , Gatz Susanne , Casanova Michela , Hjalgrim Lisa , Firth Charlotte , Wheatley Keith , Kearns Pamela , Liu Wenyu , Kirkham Amanda , Rees Helen , Bisogno Gianni , Wasti Ajla , Wakeling Sara , Heenen Delphine , Tweddle Deborah , Merks Johannes , Jenney Meriel
Published: February 2024

The Frontline and Relapsed Rhabdomyosarcoma (FaR-RMS) clinical trial is an overarching, multinational study for children and adults with rhabdomyosarcoma (RMS). The trial, developed by the European Soft Tissue Sarcoma Study Group (EpSSG), incorporates multiple different research questions within a multistage design with a focus on (i) novel regimens for poor prognostic subgroups, (ii) optimal duration of maintenance chemotherapy, and (iii) optimal use of radiotherapy for local control and widespread metastatic disease. Additional sub-studies focusing on biological risk stratification, use of imaging modalities, including [18F]FDG PET-CT and diffusion-weighted MRI imaging (DWI) as prognostic markers, and impact of therapy on quality of life are described. This paper forms part of a Special Issue on rhabdomyosarcoma and outlines the study background, rationale for randomisations and sub-studies, design, and plans for utilisation and dissemination of results.
Reply to Jue et al. Overcoming Understaging and Undergrading in Upper Tract Urothelial Carcinoma. Comment on “Ghoreifi et al. Modern Kidney-Sparing Management of Upper Tract Urothelial Carcinoma. Cancers 2023, 15, 4495”
Authors: Ghoreifi Alireza , Sari Motlagh Reza , Fuchs Gerhard
Published: February 2024

We appreciate the comments made by Jue et al [...]
Correction: Jannoo et al. Targeting and Sensitization of Breast Cancer Cells to Killing with a Novel Interleukin-13 Receptor α2-Specific Hybrid Cytolytic Peptide. Cancers 2023, 15, 2772
Authors: Jannoo Riaz , Walker William , Kanamarlapudi Venkateswarlu
Published: February 2024

In the original publication [...]
Proton Treatment Suppresses Exosome Production in Head and Neck Squamous Cell Carcinoma
Authors: Chimote Ameet A. , Lehn Maria A. , Bhati Jay , Mascia Anthony E. , Sertorio Mathieu , Lamba Michael A. , Ionascu Dan , Tang Alice L. , Langevin Scott M. , Khodoun Marat V. , Wise-Draper Trisha M. , Conforti Laura
Published: February 2024

Proton therapy (PT) is emerging as an effective and less toxic alternative to conventional X-ray-based photon therapy (XRT) for patients with advanced head and neck squamous cell carcinomas (HNSCCs) owing to its clustered dose deposition dosimetric characteristics. For optimal efficacy, cancer therapies, including PT, must elicit a robust anti-tumor response by effector and cytotoxic immune cells in the tumor microenvironment (TME). While tumor-derived exosomes contribute to immune cell suppression in the TME, information on the effects of PT on exosomes and anti-tumor immune responses in HNSCC is not known. In this study, we generated primary HNSCC cells from tumors resected from HNSCC patients, irradiated them with 5 Gy PT or XRT, and isolated exosomes from cell culture supernatants. HNSCC cells exposed to PT produced 75% fewer exosomes than XRT- and non-irradiated HNSCC cells. This effect persisted in proton-irradiated cells for up to five days. Furthermore, we observed that exosomes from proton-irradiated cells were identical in morphology and immunosuppressive effects (suppression of IFN-γ release by peripheral blood mononuclear cells) to those of photon-irradiated cells. Our results suggest that PT limits the suppressive effect of exosomes on cancer immune surveillance by reducing the production of exosomes that can inhibit immune cell function.
A Closer Look at Parental Anxiety in Asthma Outpacing Children’s Concerns: Fear of Physical Activity over the Fear of Drug Side Effects
Authors: Rogulj Marijana , Vukojević Katarina , Lušić Kalcina Linda
Published: February 2024

Background: The recognition of comorbidities is relevant for asthma management, especially if these conditions/diseases are treatable traits such as anxiety. This study aimed to explore the associations between asthma severity and child and parent asthma-related anxiety and to recognize the most common specific fears. Methods: This cross-sectional study consisted of 150 parents and their children diagnosed with asthma, and was conducted at the Pediatric Clinic of the University Hospital Center Split in Croatia. All children, from ages 3 to 17 years, underwent a thorough clinical examination. A total of 150 parents and 108 children filled out an asthma-related anxiety questionnaire in paper form. Results: Parents of children with moderate and severe asthma had higher asthma-related anxiety due to restrictions related to asthma symptoms (p = 0.032), and children diagnosed with moderate and severe asthma had greater anxiety due to restrictions related to asthma symptoms than children diagnosed with mild asthma (p = 0.004). Children’s anxiety was the highest when they experienced an asthmatic attack during physical activity (PA), and they fear that they will not be successful in sports or dancing due to asthma. Parents commonly reported the fear of an asthma attack without warning signs (p < 0.001), fear of drug side effects (p < 0.001), fear of absence from school (p = 0.006), and fear of an asthma attack during PA (p < 0.001). Conclusions: The current study reports findings of increased parental levels of anxiety when compared to their children, related to fear of an asthma attack occurring without warning signs, fear of side effects and fear of absence from school, as well as the fear of an asthma attack occurring during sports activities. When assessing individual items on anxiety associated with asthma, children most commonly reported concern related to physical activity.
Effects of Blood-Derived Products on Cellular Senescence and Inflammatory Response: A Study on Skin Rejuvenation
Authors: Kühnel Harald , Pasztorek Markus , Kuten-Pella Olga , Kramer Karina , Bauer Christoph , Lacza Zsombor , Nehrer Stefan
Published: February 2024

Blood-derived products, such as citrate platelet-rich plasma (CPRP) and hyperacute serum (HAS), are recognized for their rich growth factor content. When human dermal fibroblast (HDF) cells are exposed to combined mitogenic and DNA-damaging stimuli, it can lead to an increased burden of senescent cells and a modified senescence-associated secretory phenotype. In this study, the senescent state was comprehensively assessed through various methods, including phosphorylated histone H2AX (γH2AX) staining, p21 and p16 q-PCR, p21-western blot, growth curves, and senescence-associated ß-galactosidase staining. Two primary treatments with blood products were administered, one early (immediately after etoposide) and the other late (11 days after etoposide treatment). The effects of the blood product treatment were evaluated by measuring interleukin 6 and 8 (IL-6 and IL-8) levels, as well as collagen 1 (COL1) and p21 mRNA expression. Additionally, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assays, cell size measurements, viability assays, and cell number calculations were conducted. The results revealed that cells treated with hyperacute serum in the early treatment phase exhibited the lowest observed IL-6 and IL-8 levels. In contrast, a clear inflammatory response for IL-8 was observed in cells treated with hyperacute serum and citrate platelet-rich plasma during the late treatment. Furthermore, an upregulation of COL1 expression was observed in the early treatment, while cells in the late treatment group remained unaffected. Notably, citrate platelet-rich plasma-treated cells showed a decrease in COL1 expression. Overall, the treatment with blood products appears to have slightly positive effects on skin rejuvenation.
COVID-19 Associated Cardiovascular Disease—Risks, Prevention and Management: Heart at Risk Due to COVID-19
Authors: Kemerley Andrew , Gupta Abhishek , Thirunavukkarasu Mahesh , Maloney Monica , Burgwardt Sean , Maulik Nilanjana
Published: February 2024

The SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) virus and the resulting COVID-19 pandemic have had devastating and lasting impact on the global population. Although the main target of the disease is the respiratory tract, clinical outcomes, and research have also shown significant effects of infection on other organ systems. Of interest in this review is the effect of the virus on the cardiovascular system. Complications, including hyperinflammatory syndrome, myocarditis, and cardiac failure, have been documented in the context of COVID-19 infection. These complications ultimately contribute to worse patient outcomes, especially in patients with pre-existing conditions such as hypertension, diabetes, or cardiovascular disease (CVD). Importantly and interestingly, reports have demonstrated that COVID-19 also causes myocardial injury in adults without pre-existing conditions and contributes to systemic complications in pediatric populations, such as the development of multisystem inflammatory syndrome in children (MIS-C). Although there is still a debate over the exact mechanisms by which such complications arise, understanding the potential paths by which the virus can influence the cardiovascular system to create an inflammatory environment may clarify how SARS-CoV-2 interacts with human physiology. In addition to describing the mechanisms of disease propagation and patient presentation, this review discusses the diagnostic findings and treatment strategies and the evolution of management for patients presenting with cardiovascular complications, focusing on disease treatment and prevention.
NLU-V: A Family of Instruction Set Extensions for Efficient Symmetric Cryptography on RISC-V
Authors: Uzuner Hakan , Kavun Elif Bilge
Published: February 2024

Cryptographic primitives nowadays are not only implemented in high-performance systems but also in small-scale systems, which are increasingly powered by open-source processors, such as RISC-V. In this work, we leverage RISC-V’s modular base instruction set and architecture to propose a generic instruction set extension (ISE) for symmetric cryptography. We adapt the work from Engels et al. in ARITH’13, the non-linear/linear instruction set extension (NLU), which presents a generic hardware/software co-design solution for efficient symmetric crypto implementations through a hardware unit extending the 8-bit AVR instruction set. These new instructions realize non-linear and linear layers, which are widely used to implement the block ciphers in symmetric cryptography. Our proposal modifies and extends the NLU instructions to a 32-bit RISC-V architecture; hence, we call the proposed ISE ‘NLU-V’. The proposed architecture is integrated into the open-source RISC-V implementation ‘Icicle’ and synthesized on a Xilinx Kintex-7 XC7K160T FPGA. The area overhead for the proposed NLU-V ISE is 1088 slice registers and 4520 LUTs. As case studies, the PRESENT and AES block ciphers are implemented using the new ISE on RISC-V in assembly. Our evaluation metric to showcase the performance gain, Z ‘time-area-product (TAP)’ (the execution time in clock cycles times code memory consumption), reflects the impact of the proposed family of instructions on the performance of the cipher implementations. The simulations show that the NLU-V achieves 89% gain for PRESENT and 68% gain for AES. Further, the NLU-V requires 44% less lines of code for the PRESENT and 23% less for the AES implementation.
First Use of Free-Diving Photo-Identification of Porbeagle Shark (Lamna nasus) off the Brittany Coast, France
Authors: Jung Armelle , Ory Arthur , Abaut Paul , Zaccagnini Lucas
Published: February 2024

A large number of pelagic shark species have declined significantly in recent decades due to overfishing, bycatch, and habitat degradation. Whereas porbeagle sharks have become scarce due to a reduction in their populations around the world, recent stock evaluations are giving positive signals about the evolution of the North-Eastern Atlantic stock size. The porbeagle shark (Lamna nasus), an offshore pelagic species with a wide distribution, is designated by IUCN as Globally Vulnerable and Critically Endangered for Europe and subject to various international conservation conventions. An increasing number of observations are reported off the Brittany coast of Trégor. The ecological role of this area for the species is still unknown and greater knowledge is needed to develop and apply sustainable management measures on a local and international scale. This study represents the first use of photo-identification on porbeagle sharks in order to improve the ecological knowledge of the species in the Trégor area. These results confirm the effectiveness of this method, with 19 of the 131 individuals identified being re-sighted, indicating an interesting degree of site fidelity and showing a sex ratio of 100% females. Observations of individuals over several years allowed the researchers to discuss the relevance of the different types of marks. The findings suggest that the Trégor area off the Brittany coast serves as a seasonal residence for female porbeagle sharks, especially between May and October. This study represents a successful first step in the use of photo-identification for this species. It offers technical support for the sharing of the methodology and provides some biological knowledge allowing researchers to discuss potential sustainable management measures for the conservation of porbeagle sharks in the study area and their habitats while needed.
Multipartite Entanglement: A Journey through Geometry
Authors: Xie Songbo , Younis Daniel , Mei Yuhan , Eberly Joseph H.
Published: February 2024

Genuine multipartite entanglement is crucial for quantum information and related technologies, but quantifying it has been a long-standing challenge. Most proposed measures do not meet the “genuine” requirement, making them unsuitable for many applications. In this work, we propose a journey toward addressing this issue by introducing an unexpected relation between multipartite entanglement and hypervolume of geometric simplices, leading to a tetrahedron measure of quadripartite entanglement. By comparing the entanglement ranking of two highly entangled four-qubit states, we show that the tetrahedron measure relies on the degree of permutation invariance among parties within the quantum system. We demonstrate potential future applications of our measure in the context of quantum information scrambling within many-body systems.
Dog-Assisted Therapy in Mental Health Care: A Qualitative Study on the Experiences of Patients with Intellectual Disabilities
Authors: Schooten Anke , Peters-Scheffer Nienke , Enders-Slegers Marie-José , Verhagen Inge , Didden Robert
Published: February 2024

(1) Background: Dog-assisted therapy (DAT) is an experiential intervention to promote psychological, physical, and social functioning in children and adults. Only few studies have been conducted on DAT in adults with a mild intellectual disability or borderline intellectual functioning (MID-BIF). The purpose of this study was to explore the experiences of patients with MID-BIF undergoing DAT in a mental health care facility. (2) Method: Seven patients completed 13 to 15 sessions of DAT. Within two weeks of completing the program, they were interviewed using a semi-structured interview. The transcripts of the interviews were analysed using interpretational phenomenological analysis. In addition, the patients’ relatives and the DAT therapist were interviewed, and a focus group discussion took place with each patient’s treatment team. (3) Results: The patients’ experiences were predominantly positive. Physical contact with the dog calmed them down. The dog offered them emotional support and helped them to make contact inside and outside the therapy and the setting where they lived. The patients also liked the fact that DAT focused on the dog rather than their problems, that the therapy was experiential and using a positive approach, and that, during the therapy, they did not feel like a patient but a human being. DAT is a promising therapy for patients with MID-BIF in mental health care facilities, but more research into its effectiveness and cost-effectiveness and ways to implement DAT in clinical practice is needed to make more definitive statements.
Porous Prussian Blue Analogs Decorated Electrospun Carbon Nanofibers as Efficient Electrocatalyst for Overall Water Splitting
Authors: Xiao Zhiqing , Zhu Xiubin , Bai Lu , Liu Zhicheng
Published: February 2024

Metal-organic frameworks are becoming increasingly important in electrocatalysis as the hydrogen production sector grows. However, their electrocatalytic capability is limited by their inclination to agglomerate and the insufficient exposure of active sites. In this work, a three-step strategy was used to develop a bifunctional electrocatalyst with porous Prussian blue analogs supported on carbon nanofibers. The use of electrospun carbon nanofibers as conductive substrates can successfully address the problem of easy aggregation. Moreover, the etching procedure with tannic acid creates a porous structure that effectively regulates the electrical structure and exposes additional active sites. The resulting catalyst performs well in both the hydrogen evolution reaction and the oxygen evolution reaction, and also exhibits good stability in overall water splitting. The findings of this study present new concepts for the design and fabrication of metal-organic frameworks-based materials in the realm of electrocatalysis.
Electromechanical Energy-Based 3D-Controllable Motion of Small Matter toward Tiny Machines
Authors: Manamanchaiyaporn Laliphat , Tang Xiuzhen
Published: February 2024

Is it possible to remotely operate a tiny piece of matter or a less-than-one-centimeter machine to perform a medical task in life? Especially given that in the present technology, neither the mechanism nor battery is small enough to be set up inside the structure of such a tiny machine. Yet, if the powered matter is magnetically responsive, then a magnetic field, as one of the potential power sources, can be applied to power it promisingly. Herein, the concept of electromechanical energy conversion is utilized through a specific configuration consisting of eight solenoids arranged together as a nest. The device converts electrical energy into an electromagnetic field, and finally, into mechanical energy, respectively, resulting in magnetic manipulation. Since electric energy is supplied to the configuration, eight solenoids generate the controllable magnetic field in both direction and magnitude by means of the superposition technique. The device can magnetically navigate tiny motorless matter to release mechanical energy through the 3D-controllable motion to arbitrary positions effectively and physical interactions with the surrounding environment as if operating a tiny machine. The experimental results report the feasibility of the device to control the 6-DOF locomotion of small matter precisely. The contribution of the concept based on this work leads to a promising protocol to remotely power small machines, micro-engines, micro-propellers, micro-turbines, etc.
The Energy Transition in SMEs: The Italian Experience
Authors: Thomas Antonio , Castellano Rosalia , Punzo Gennaro , Scandurra Giuseppe
Published: February 2024

Encouraging energy transition (ET) has become a global imperative for nations and companies, and not just large ones. Not all economic organizations pursue this process with the same intensity. Particularly, the factors driving Small and Medium Enterprises (SMEs) towards ET are not yet clearly defined, despite SMEs representing most companies and bearing a significant responsibility for pollution. To shed light on this matter, this study presents a framework of the main drivers that stimulate SMEs placed in Italy to pursue energy transition. We conducted a Partial Least Squares (PLS) analysis on data collected from innovation-oriented SMEs enlisted in a specific register of the Italian Ministry of Economic Development. The research findings show a weak interest among SMEs towards ET. While country-specific conditions and social and psychological traits affect the decision to invest in ET, firm-specific factors and ‘stakeholder’ pressures seem to be almost irrelevant. Policy measures with a different focus are necessary. Specifically, the provision of facilitation emerges as the most effective tool for encouraging ET among SMEs.
Fundamental Components and Principles of Supervised Machine Learning Workflows with Numerical and Categorical Data
Authors: Kampezidou Styliani I. , Tikayat Ray Archana , Bhat Anirudh Prabhakara , Pinon Fischer Olivia J. , Mavris Dimitri N.
Published: February 2024

This paper offers a comprehensive examination of the process involved in developing and automating supervised end-to-end machine learning workflows for forecasting and classification purposes. It offers a complete overview of the components (i.e., feature engineering and model selection), principles (i.e., bias–variance decomposition, model complexity, overfitting, model sensitivity to feature assumptions and scaling, and output interpretability), models (i.e., neural networks and regression models), methods (i.e., cross-validation and data augmentation), metrics (i.e., Mean Squared Error and F1-score) and tools that rule most supervised learning applications with numerical and categorical data, as well as their integration, automation, and deployment. The end goal and contribution of this paper is the education and guidance of the non-AI expert academic community regarding complete and rigorous machine learning workflows and data science practices, from problem scoping to design and state-of-the-art automation tools, including basic principles and reasoning in the choice of methods. The paper delves into the critical stages of supervised machine learning workflow development, many of which are often omitted by researchers, and covers foundational concepts essential for understanding and optimizing a functional machine learning workflow, thereby offering a holistic view of task-specific application development for applied researchers who are non-AI experts. This paper may be of significant value to academic researchers developing and prototyping machine learning workflows for their own research or as customer-tailored solutions for government and industry partners.
Development of Method for the Measurement of Dermal Exposure on a Lifelike Cow Dummy to Assess the Risk of Using a Urease Inhibitor (Atmowell®) to Reduce NH3 Emissions in Cattle Farming
Authors: Ehmke Annika , Wegener Jens Karl , Melfsen Andreas , Hartung Eberhard
Published: February 2024

Up to 58% of NH3 emissions can be reduced through the use of the urease inhibitor Atmowell® in cattle farming. Automated manure scraper and drag hose systems should be used to apply urease inhibitors (UIs) to stable floors. Proof of safe use is also needed. A risk assessment of the urease inhibitor PPDA was conducted utilising estimated and calculated exposure data along with established and verified model calculation tools. Risk assessment models can be improved using measured dermal exposure data. The fluorescent dye pyranine and a Tyvek® collector were used to measure exposure. Tyvek® was attached to a lifelike cow dummy. Regarding the drag hose system, two worst-case scenarios were considered: over the cow and passing the cow. Regarding the robot application system, a 0 m frontal scenario was considered. The over the cow scenario exhibited the highest dermal exposure, with 1.5 mg of PPDA/cow per application run. The robot scenario exhibited the smallest amount, with 0.8 mg of PPDA/cow per application run. The risk of using PPDA was already excluded by model calculation tools in a risk assessment. Compared with the estimated and calculated dermal exposure values, the measured dermal exposure levels were reduced by a factor of two to six.