bluuebunny/crossref_metadata_2025 huggingface dataset:
87 Effect of Number of Source Litters Used to Create Cross-fostered Litters on Piglet Pre-weaning Mortality and Weaning Weight
Authors: Vande Pol Katherine D , Rivas Rafael Ovidio Bautista , Espinal Alicia Olivo , Harper Heath , Shull Caleb M , Brown Katie , Ellis Michael
Published: May 2021

Sow litter sizes have increased over recent decades, increasing the need for cross-fostering. The objective of this study was to determine the effect of the number of source litters used to create cross-fostered litters on piglet pre-weaning mortality (PWM) and weaning weight. A RCBD was used with 26 blocks of 5 litters (total 130 litters/1820 piglets), all litters consisted of 14 piglets. Blocking factors were farrowing day, sow parity, body condition score, and functional teat number, and the average and CV of piglet birth weight. Five cross-fostering treatments were compared: 0%, 1 source (all piglets remaining on the birth sow); 100%, 1 source (all piglets moved from birth to a different sow); 100%, 6+ sources (piglets from ≥ 6 birth sows used to form a litter on a different sow); 50%, 2 sources (7 piglets remaining with birth sow, 7 from one other sow); 50%, 4+ sources (7 piglets remaining with the birth sow, 7 from ≥ 3 other sows). The single-source litters were selected from those with > 14 piglets at birth, with excess piglets removed. For other treatments, piglets were selected to meet blocking factors. Piglets were weighed 24 h after birth and at weaning (19.5 ± 0.50 d); all PWM was recorded. Weight data were analyzed using PROC MIXED of SAS; PWM data were analyzed using PROC GLIMMIX of SAS. Models included Treatment and sow within block. There was no effect (P > 0.05) of treatment on weaning weights. Pre-weaning mortality was greater (P < 0.05) for the 0%, 1 source compared to the 50%, 2 source treatment, with the others being intermediate and generally not statistically different (Table 1). In conclusion, cross-fostering and/or mixing litters had no effect on weaning weights, but pre-weaning mortality was highest for the non-fostered treatment.
233 Effects of Harvest Maturity And/or Kernel Processing on Corn Silage Processing Score and Particle Size of Corn Silage
Authors: Hamilton Thomas , Walker Julie , Rusche Warren C , Smith Zachary K
Published: May 2021

A single corn hybrid was used to evaluate harvest maturity (Mat) and/or kernel processing (KP) effects on corn silage processing score (CSPS) and particle size (PS). Treatments were arranged in a 2 × 2 factorial of 1) Mat (early and late) and 2) KP (no or yes). A single corn field was planted on April 27, 2020. There were 12 loads (experimental unit) per simple effect treatment mean. Data were analyzed as a completely randomized design. Early harvest occurred on August 28, 2020 [yield (as is) = 39.1 Mg/hectare; DM = 43.1%; CP, NDF, and starch = 6.5, 46.0, and 32.9%, respectively (DM basis)]. Late harvest occurred on September 9, 2020 [yield = 37.8 Mg/hectare (as is); DM = 49.2%; CP, NDF, and starch = 6.6, 49.8, and 37.5%, respectively (DM basis)]. The same equipment was used for both Mat with KP achieved by narrowing processing rollers. The CSPS was determined as the proportion of starch retained below a 4.75-mm sieve. Grain content (DM basis) of the corn silage was calculated from starch/0.72. Particle size was assessed using the Penn State Particle Separator. A Mat × KP interaction (P = 0.05) was detected for CSPS. Early/no and late/no had decreased (P ≤ 0.05) CSPS compared to early/yes and late/yes had the greatest CSPS (P ≤ 0.05) compared to others. Grain content was 13.9% greater in late compared to early (P = 0.01). A Mat × KP interaction (P = 0.03) was detected for PS. Early/no had the greatest (P ≤ 0.05) PS, early/yes and late/no were intermediate, and late/yes had decreased PS compared to others (P ≤ 0.05). These data indicate that Mat and KP influence CSPS synergistically. Producers should consider KP when corn silage is harvested at a later maturity to enhance CSPS.
235 Resource Use for Beef Cattle in the North Central Great Plains
Authors: Lakamp Andrew D , Aherin Dustin , Spencer Douglas , Larson Robert , Weaber Robert L , Bormann Jennifer M , Rolf Megan M
Published: May 2021

The sustainability of the beef industry has become a point of national interest, particularly the investment of land and water resources. Our objective was to estimate how much land and irrigation water are required to maintain a simulated Angus cow-calf operation in the North Central Great Plains (NCGP) for an average year. A stochastic model was used, which enabled consideration of biological variation. The model computed 100 iterations of a 24-year timeframe (1995–2018). The simulated herd had 100 breeding females with replacement heifers being retained annually. The nutrients required to maintain a body condition score 5 for each individual animal, adjusting for temperature and physiological state, were calculated. A stocking rate of 3.3 hectares (ha) per cow-calf pair and mature cow weight of 600 kg was set, which is representative of the NCGP. Replacement heifers were assumed to be 65% of mature cow weight and allotted 1.22 ha. Bred heifers were assumed to be 85% of mature cow weight and allotted 1.81 ha. The herd was assumed to be grazing from May 1 to October 31. A supplemented ration of 60% alfalfa and 40% corn was provided if an individual’s nutritional needs were not met. Animals were assumed to be delivered a base ration from November 1 to April 30, which consisted of 73% alfalfa, 19% wheat straw, and 8% corn. The amount of irrigation necessary to grow feed was determined by estimating evapotranspiration of each crop then subtracting the amount of precipitation during the growing season. Average crop yield was determined using county level data from the UDSA NASS to estimate how much land would be needed for feed production. Sustaining a 100 head cow-calf herd in the NCGP for an average year requires 103.5 million liters for irrigation, 1288.5 ha for crop production, 357 ha grazing land.
9 What Would Be the Next Feed Amino Acid Based on a Microbial Point of View?
Authors: Kim So-Young
Published: May 2021

Global Amino acids market is estimated as $15billion in 2020 and 60% of those volume is used for feed additives. Amino acids are converted to protein efficiently and have a benefit of lowering feeding cost and reducing the nitrogen content of the waste output compared to intact protein diet. Most amino acids are produced using microbial fermentation. Traditionally, amino acids used for feed additives are Lysine, threonine, tryptophan and methionine. Among them methionine was produced only by chemical process, but now methionine can also be produced by microbial fermentation, which can enlarge the choice of methionine from DL-form to L-form. Use of L-form methionine as feed additives reveals new nutritional value of L-methionine compared to DL-methionine. Recently, valine, arginine, isoleucine, and histidine are also included in feeds as functional nutrients thanks to their commercial availability and lower price. Technical progress in microbiology and bioengineering has been made more diverse amino acid to be used as feed additives. Then what would be the next amino acid as feed additivities based on microbial point of view? Leucine, glutamine, tyrosine and phenylalanine could be next possible amino acids based on metabolic pathways of microorganism. These amino acids share the pathways with former feed amino acids such as valine or tryptophan, which means that these can be produced with way that is more economical. In addition, these amino acids could be produced as mixed forms like glutamic acid/glutamine mixture, Tryptophan/tyrosine/phenylalanine mixture, or BCAA forms with cheaper price. Dried fermentation product such as lysine sulfate made by drying of microbial culture broth, could be one of the option for the combination of several new amino acids. Crude protein reduction in feed is one of the big nutritional trend to reach the ideal protein diet and environmental clean situation. Considering the use of new synthetic amino acids in feeds would be a potential way to realize the ideal protein diet.
4 Protein and Amino Acid Concepts and Use in Swine Nutrition: Gary Allee’s Contributions to the Swine Industry
Authors: Goodband Robert D
Published: May 2021

I was very fortunate to meet Gary Allee in 1984 when I began my M.S. degree at Kansas State University. I’ll remember Gary most in that he cared about people and truly wanted to serve humanity and make the world a better place through animal agriculture. He helped develop scholars, both nationally and internationally, that would be the future of our industry. Gary was proficient in seeking a solution to a problem and finding the experimental resources to explain it. Very early in his career, he helped verify the concept of a lysine:calorie ratio as a means of explaining the previously varied and inconsistent response to added fat in swine diets. Early research outlined the order of limiting amino acids in various feed ingredients. Gary’s research also focused on determining the nutritional value of protein sources for weanling pigs, such as dried whey, fish meal and dried skim milk that ultimately led to phase feeding strategies for early weaned pigs that are the backbone of our industry. Gary and his students determined lysine and other amino acid requirements for growing pigs and sows under field conditions. He helped elucidate the effects of low-protein, amino acid fortified diets under heat stress environments. As market weights increased, his research was instrumental in determining how to feed heavy weight pigs as well as those fed ractopamine. Gary grasped concepts and applied them into practical solutions in swine nutrition. He was a strong believer and leader in cooperative research among universities but also, at that time, a novel concept of university-industry partnerships. Those of us that can say they knew Gary Allee, are very fortunate and better because of it.
130 Utilization of Nutrack to Assess Variance Components and Heritability of Activity Traits
Authors: Ostrand Lexi M , Trenhaile-Grannemann Melanie D , See Garrett , Schmidt Ty B , Psota Eric , Rohrer Gary A , Mote Benny E
Published: May 2021

Overall activity and behavior are integral components of sows remaining productive in the herd. This investigation studied overall activity of group housed replacement gilts and the heritability of various activity traits. Beginning around 20 wk of age, video recorded data of approximately 75 gilts/group for a total of 2,378 gilts over 32 groups was collected for 7 consecutive d using the NUtrack System, which tracks distance travelled (m), avg speed (m/s), angle rotated (degrees), and time standing (s), sitting (s), eating (s), and laying (s). The recorded phenotypes were standardized to the distribution observed within a pen for each group. The final values used for analysis were the average daily standardized values. Data were analyzed using mixed models (RStudio V 1.2.5033) including effects of sire, dam, dam’s sire and dam, dam’s grandsire and granddam, farrowing group, barn, pen, and on-test date. Sire had an effect on every activity trait P < 0.001), and dam had an effect on average speed (P < 0.001). The dam’s sire had an effect on all activity traits (P < 0.001) and the dam’s grandsire had an effect on average speed (P < 0.001). Heritabilities and variance components of activity traits were estimated in ASReml 4 using an animal model with a two-generation pedigree. Genetic variances are 0.17 +/- 0.029, 0.19 +/- 0.034, and 0.11 +/- 0.024, residual variances are 0.37 +/- 0.023, 0.41 +/- 0.027, and 0.41 +/- 0.022, phenotypic variances are 0.54 +/- 0.018, 0.60 +/- 0.020, and 0.52 +/- 0.016, and heritabilities are 0.32 +/- 0.048, 0.32 +/- 0.049, and 0.21 +/- 0.044 for average speed, distance, and lie respectively. NUtrack offers potential to aid in selection decisions. Given the results presented herein, continued investigation into these activity traits and their association with sow longevity is warranted.
129 Genomic Relationship Between PRRSV Wild-type Infection and PRRSV Vaccination for Antibody Response and Reproductive Performance
Authors: Sanglard Leticia P , Hickmann Felipe , Huang Yijian , Gray Kent A , Linhares Daniel , Dekkers Jack C , niederwerder Megan , Fernando Rohan , Serão Nick V
Published: May 2021

Immunoglobulin G antibody response, measured as sample-to-positive (S/P) ratio, to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) has been proposed as an indicator trait for improved reproductive performance in PRRSV-infected purebred sows and PRRSV-vaccinated crossbred gilts. In this study, we investigated the genetic correlations (rg) of S/P ratio following a PRRSV outbreak and PRRSV-vaccination with performance in non-exposed and PRRSV-exposed sows. PRRSV outbreak phase was defined based on previously described methodologies after the detection of typical clinical signs of PRRSV infection. 541 Landrace sows had S/P ratio measured at ~54 days after the beginning of the PRRSV outbreak (S/Poutbreak), and 906 Landrace x Large White naïve F1 gilts had S/P ratio measured at ~50 days after vaccination with a commercial modified live PRRSV vaccine (S/PVx). 711 and 428 Landrace sows had reproductive performance recorded before and during the PRRSV outbreak, respectively. 811 vaccinated F1 animals had farrowing performance for up to 3 parities. All animals were genotyped for ~28K SNPs. The estimate of rg of S/Poutbreakwith S/PVx was high (rg±SE = 0.72±0.18). Estimates of rg of S/Poutbreak with reproductive performance in F1 sows were low to moderate, ranging from 0.05±0.23 (number stillborn) to 0.30±0.20 (total number born). Estimates of rg of S/PVxwith reproductive performance in non-infected purebred sows were moderate and favorable with number born alive (0.50±0.23), but low (0 to -0.11±0.23) with litter mortality traits. Estimates of rg of S/PVx were moderate and negative (-0.47±0.18) with the number of mummies in PRRSV-infected purebred sows and low with other traits (-0.29±0.18 for total number born to 0.05±0.18 for number stillborn). These results indicate that selection for antibody response following a PRRSV outbreak collected in purebred sows and to PRRSV vaccination collected in commercial crossbred gilts may increase litter size of non-infected and PRRSV-exposed purebred and commercial crossbred sows.
128 Differences in PRRSV Resilience for Reproductive Performance Between Landrace and Duroc Sows
Authors: Hickmann Felipe , Neto José Braccini , Kramer Luke M , Gray Kent A , Huang Yijian , Dekkers Jack C , Sanglard Leticia P , Serão Nick V
Published: May 2021

Studies on differences in resilience to porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) between breeds are scarce in the literature. Thus, the objective of this work was to assess PRRSV resilience in PRRSV wild-type infected sows from two breeds. Farrowing data included 2546 and 2522 litters from 894 Duroc and 813 Landrace sows, respectively, which were housed together and experienced the same PRRSV outbreak. Traits used for this study were number of piglets born alive (NBA), number born dead (NBD), total number born (TNB), and number weaned (NW). The impact of PRRSV infection was evaluated by comparing the reproductive performance of breeds between PRRS phases (pre-PRRS, PRRS, and post-PRRS). PRRS phases were defined based on the reproductive performance data. NBA, NBD, and NW were analyzed as a proportion of TNB using a Poisson mixed model. Pre-defined contrasts were used to evaluate the effect of breed on PRRSV resilience and on return to PRRSV-free performance, representing the differences between breeds for the difference between pre-PRRS and PRRS phases, and pre-PRRS and post-PRRS phases, respectively. There was a significant (P ≤ 0.003) interaction between PRRS phase and breed for all traits, as shown in Table 1. In general, reproductive performance reduced from pre-PRRS to PRRS, and then increased from PRRS to post-PRRS, as expected. The resilience contrast was significant for all traits (P ≤ 0.003). In all cases, the drop in percent reproductive performance from pre-PRRS to PRRS was lower for Duroc than for Landrace, indicating that Duroc sows have greater PRRSV resilience than Landrace sows. The return to PRRSV-free performance contrast had a trending effect for NBD (P = 0.055), and it was not significant for the other traits (P ≥ 0.515). These results indicate that Duroc sows have overall greater phenotypic PRRSV resilience for reproductive performance than Landrace sows.
24 Effects of Spring versus Fall Calving on Fetal Growth, Vigor at Birth, and Neonatal Circulating Metabolites in Beef Calves
Authors: Wichman Lindsey G , Redifer Colby A , Meyer Allison M
Published: May 2021

To determine the effect of season on perinatal nutrient availability, assessed through fetal growth, calf vigor, and neonatal circulating metabolites, multiparous and primiparous dams (age: 4.7 ± 2.4 yr; BCS: 5.3 ± 0.6) from 4 spring (2014–2017; n = 202) and 4 fall (2015, 2017–2019; n = 177) calving experiments were observed during parturition. Time to stand (spring: 67; fall: 104) was determined as minutes from birth to standing for 5 sec. After birth, calf BW and size (spring: 99; fall: 169; length, heart girth, abdominal girth, and cannon circumference) were recorded. Jugular blood samples were obtained from 63 spring and 89 fall calves at 0 (pre-suckling), 6, 12, 24, and 48 h postnatally. Data were analyzed either with the fixed effect of season (single point) or the fixed effects of season, hour, and their interaction with hour as a repeated effect (over time); calf sex was included when P < 0.25. Experiment was a random effect. Fall-born calves tended to have lighter (P = 0.09) BW and faster (P = 0.05) time to stand than spring-born calves. Season did not affect (P ≥ 0.18) other calf size measures. The season x hour interaction (P ≤ 0.07) affected circulating glucose, non-esterified fatty acids (NEFA), triglycerides, urea nitrogen, globulin, and total protein. Spring-born calves had greater (P ≤ 0.009) 0 h glucose, 0 and 6 h NEFA, and 0, 6, 12, and 48 h triglycerides than fall-born calves. Fall-born calves had greater (P = 0.03) total protein at 24 h and tended to have greater (P ≤ 0.10) total protein and urea nitrogen at 48 h and globulin at 24 h. Season affected albumin, which was greater (P = 0.003) in fall-born calves. These data suggest that calving season influences perinatal nutrient availability, which may impact the transition to postnatal life.
22 Effects of Housing Cow-calf Pairs on Drylots vs Pasture on Calf Performance and Behavior Through Weaning
Authors: Myerscough Megan , Chapple Wes , Meteer William T , Shike Daniel W
Published: May 2021

The objectives were to analyze the effects of housing cow-calf pairs in drylots or pasture on calf performance and behavior through weaning. Simmental × Angus (2 yr; 108/yr; 81 ± 15.3 d postpartum) spring-calving cows were stratified by age, BW, BCS, and calf sex and allotted to six groups/yr. Groups were randomly allotted to 1 of 2 treatments: drylot (DL) or pasture (PAST). Calves in the drylot had ad libitum access to a diet consisting of corn silage, dried distillers grain, corn stalks, dry rolled corn, and soybean hulls. Calves on PAST received creep feed three weeks prior to weaning. Calf BW was measured on d 0, 55, and 110. Calves were fence-line weaned on d 110. Behavior was observed for two days after weaning. Data were analyzed using the MIXED procedure of SAS. Drylot calves had greater (P < 0.01) BW at d 55 and 110. There was treatment by time interactions (P < 0.01) for lying and eating on d 111. More PAST calves were lying at h 2 and 3 and eating at h 11 and 12. More DL calves were lying at h 9, 10, 11, and 12. More DL calves were eating at h 2 and tended to at h 3. There was also a treatment by time interaction (P < 0.01) for vocalizations on d 111. More PAST calves vocalized at h 1, however, DL calves vocalized more at h 4 and tended to at h 5. There was treatment by time interactions (P < 0.01) for eating and walking on d 112. More DL calves were eating at h 2, 3, 4, 7, and 9. More PAST calves were walking at h 1 and 2. There was also a treatment by time interaction (P < 0.01) for vocalizations on d 112. More PAST calves vocalized at h 1 and 10. In conclusion, housing cow-calf pairs in drylots improved preweaning performance and altered postweaning behavior.
27 Greenhouse Gas Emissions and a Partial Life Cycle Assessment When Growing Pigs Are Fed High Wheat Millrun Diets
Authors: Kpogo Agbee L , Jose Jismol , Panisson Josiane , Predicala Bernardo , Alvarado Alvin , Agnew Joy , Sprenger Charley , Beaulieu Denise A
Published: May 2021

The impact of feeding growing pigs with high wheat millrun diets on the global warming potential (GWP) of pork production was investigated. In study 1, a 2 × 2 factorial arrangement of wheat millrun (0 or 30%) and multi-carbohydrase enzyme (0 or 1 mg kg-1) as main effects was utilized. For each of 16 reps, 6 pigs (60.2±2.2 kg BW) were housed in environmental chambers for 14d. Air samples were collected and analyzed for carbon dioxide (CO2); nitrous oxide (N2O); and methane (CH4). In study 2, data from study 1 and performance data obtained from a previous feeding trial were utilized in a life cycle assessment (LCA) framework that included feed production. The Holos farm model (Agriculture and Agri-Food Canada, Lethbridge. AB) was used to estimate emissions from feed production. In study 1, total manure output from pigs fed 30% wheat millrun diets was 30% greater than pigs on the 0% wheat millrun diets (P < 0.05), however, Feeding diets with 30% millrun did not affect greenhouse gas (GHG) output (CH4, 4.7, 4.9; N2O, 0.45, 0.42; CO2, 1610, 1711; mg s-1 without or with millrun inclusion, respectively; P > 0.78). Enzyme supplementation had no effect on GHG production (CH4, 4.5, 5.1; N2O, 0.46, 0.42; CO2, 1808, 1513; mg s-1 without or with enzymes, respectively; P > 0.51). In study 2, the LCA indicated that the inclusion of 30% wheat millrun in diets for growing pigs resulted in approximately a 25% reduction in GWP when compared to the no wheat millrun diets. Our results demonstrate that 30% wheat millrun did not increase GHG output from the pigs, and thus the inclusion of wheat millrun in diets of growing pigs can reduce the GWP of pork production.
28 In-feed or In-water Antibiotic Administration Did Not Influence the Fecal Prevalence and Antimicrobial Susceptibility Profiles of Salmonella in Piglets
Authors: Ishengoma Victor L , Amachawadi Raghavendra G , Shi Xiaorong , Mahmood Taghreed , Hutchens Wade M , Tokach Mike D , Dritz Steve S , Woodworth Jason C , Goodband Robert D , DeRouchey Joel M , Nagaraja T G
Published: May 2021

A total of 1,296 weaned piglets were used in a 35-d study to assess the impact of in-feed vs in-water administrations of chlortetracycline (CTC) and or tiamulin on prevalence and antimicrobial resistance (AMR) profiles of Salmonella enterica. Piglets were allocated to 48 pens (27 pigs per pen) and pens were assigned randomly to six treatment groups: control (no antibiotic), in-feed CTC, in-water CTC, in-feed tiamulin, in-water tiamulin, and in feed CTC and tiamulin. Fresh fecal samples were collected randomly from 5 of 27 piglets from each pen on days -7, 0 (pre-treatment), 7, 14 (treatment), 21, and 28 (post-treatment). Salmonella isolation and identification were done by enrichment, plating on selective medium, and species confirmation of putative colonies by PCR. Antimicrobial susceptibility and resistance of the isolates were determined using premade antibiotic panel (SensititreTM CMV3AGNF and BOPO7F) and results were interpreted based on the Clinical and Laboratory Standards Institute guidelines. All Salmonella isolates were identified as serotype Typhimurium. The overall prevalence of Salmonella was 3.0% (43/1,440) with no treatment effect (P > 0.05). All isolates were resistant (100%) to tetracycline and tiamulin. Additionally, the isolates were resistant to ampicillin (100%), streptomycin (100%), sulfisoxazole (100), ciprofloxacin (95.4%) and nalidixic acid (74.4%). Only a few isolates were resistant to trimethoprim/sulphamethoxazole (4.7%), ceftriaxone (7.0%), and ceftiofur (7.0%). PCR assays indicated the presence of tetB gene in all isolates, while 11 (25.6%) and 4 (9.3%) isolates were positive for tetD and tetA genes, respectively. Neither in-feed nor in-water administration of CTC or tiamulin impacted the fecal prevalence and antimicrobial susceptibility of Salmonella in nursery piglets.
17 A De novo Recessive Mutation Causative of Mandibulofacial Dysostosis in Hereford Cattle
Authors: Sieck Renae L , Fuller Anna M , Bedwell Patrick , Ward Jack , Sanders Stacy , Xiang Shi-Hua , Peng Sichong , Steffen David , Petersen Jessica L
Published: May 2021

In spring 2020, six Hereford calves presented with congenital craniofacial abnormalities attributed to a condition we termed mandibulofacial dysostosis (MD). Pedigree analysis revealed a single common ancestor shared by the sire and dam of each affected calf. We hypothesized that MD in Hereford cattle is attributed to a de novo mutation with an autosomal recessive mode of inheritance. To avoid production of affected calves, the objective of this study was to identify the cause of MD. Whole-genome sequencing (WGS) of 20 animals (3 affected, 7 obligate carriers, 10 related) yielded 143 variants matching the hypothesized mode of inheritance. Genotyping of 2 additional affected calves, 760 Herefords, and evaluation of WGS data from over 2,500 other individuals led to the discovery of a missense mutation (Chr26 g. 14404993 T >C) in CYP26C1 associated with MD. The amino acid change due to the CYP26C1 variant (p. L188P) is located in an α helix of the protein; modeling suggests the substitution breaks the helix. The mutation is predicted to be deleterious (SIFT = 0) and is otherwise conserved across species. In our data, all heterozygotes had at least one pedigree tie to the suspect founder. CYP26C1 plays a vital role in tissue-specific regulation of retinoic acid (RA) during embryonic development. Dysregulation of RA can result in teratogenesis by altering the endothelin-1 signaling pathway affecting the expression of Dlx genes, critical to mandibulofacial development. Further, multiple human conditions with similar pathologic characteristics are attributed to dysfunction of this gene and/or retinoic acid signaling. We conclude that this recessive missense mutation in CYP26C1 impacts the catalytic activity of the encoded enzyme, leading to excess RA and resulting in the MD phenotype. Breeders can now genotype their animals to identify carriers. These data also contribute to expanding the understanding of craniofacial development across species.
29 Live Yeast and Yeast Extracts with and Without Pharmacological Levels of Zinc on Nursery Pig Growth Performance and Fecal Escherichia coli Antimicrobial Resistance
Authors: Chance Jenna A , DeRouchey Joel M , Gebhardt Jordan T , Amachawadi Raghavendra G , Ishenhoma Victor , Nagaraja T G , Calderon Hilda I , Tokach Mike D , Woodworth Jason C , Goodband Robert D , Loughmiller Joseph
Published: May 2021

A total of 360 barrows (DNA 200×400; initially 5.6 kg) were used to evaluate yeast-based probiotics (Phileo by Lesaffre, Milwaukee, WI) in diets with or without pharmacological levels of Zn on growth and fecal Escherichia coli antimicrobial resistance (AMR). There were 5 pigs/pen and 18 pens/treatment. Dietary treatments were arranged in a 2×2 factorial with main effects of yeast pre- and probiotics (0 vs. 0.10% Actisaf Sc 47 HR+, 0.05% SafMannan, and 0.05% Nucleosaf in phase 1 then concentrations were lowered by 50% in phase 2) and pharmacological levels of Zn (110 vs. 3,000 mg/kg in phase 1 and 2,000 mg/kg in phase 2 provided by zinc oxide). Treatments were fed in two phases from d 0 to 7 and 7 to 21 with a common diet fed from d 21 to 42 post-weaning. There were no probiotics×Zn interactions. From d 0 to 21, pigs fed pharmacological Zn had increased (P < 0.001) ADG and ADFI; however, there were no effects of added pre- and probiotics. Fecal samples were collected on d 4, 21, and 42 from the same three pigs/pen for AMR profiles and fecal dry matter (DM). On d 4, pigs fed pharmacological Zn had greater fecal DM (P = 0.043); however, no differences were observed on d 21 or 42. E. coli was isolated from fecal samples and species confirmation was accomplished by PCR detection of uidA and clpB genes. Microbroth dilution method using SensititreTM CMV3AGNF panel was used to determine antimicrobial susceptibilities of E. coli isolates to 14 different antimicrobials. There was no evidence for differences in AMR of fecal E. coli isolates to antibiotics by added pre- and probiotics or Zn. Results suggest that pharmacological levels of Zn stimulate intake and growth and improve fecal consistency in the nursery with no statistical response from added pre- and probiotics.
26 Extending the Shelf-life of Beef Bone-in Short Rib Steaks Using Acerola Cherry Powder and Rosemary Extract
Authors: Van Buren Jessie B , Buseman Brianna , Weber Tanya , Nasados James , Lancaster Jessica M , Smart Jaxon , Bass Phillip , Colle Michael
Published: May 2021

Improving the shelf-life of beef bone-in short rib steaks, which are commonly exported, will increase beef export demand and subsequently producer profits. The objective was to determine the effect of the topical application of acerola cherry powder and rosemary extract from various suppliers on beef bone-in short rib shelf-life. Beef bone-in short ribs (IMPS 123A) (n = 18) from USDA Choice carcasses were aged for 28 days post-fabrication at 0°C. Steaks 1.02 cm-thick were systematically assigned based on location to treatments including: untreated control (C), topically sprayed (~2ml) with an acerola cherry powder solution (0.05%) from one of three suppliers (C1, C2, C3), or topically sprayed (~2ml) with a rosemary extract solution (0.10%) from one of three suppliers (R1, R2, R3). Steaks were assigned to day 0 lipid oxidation or 4 days of retail display followed by day 4 lipid oxidation. Steaks were weighed on day 0 and 4 to determine fluid loss. Throughout retail display, objective and subjective color were measured twice daily on the lean and bone marrow portions of the steaks. Data were analyzed using the Mixed Model procedure of the Statistical Analysis System. Lipid oxidation (P = 0.323) did not differ between treatments. However, treatments differed in fluid loss (P = 0.024), where steaks treated with C1, C2, C3, R2, and R3 had less fluid lost than control steaks. Subjective color evaluation of lean color (P < 0.0001) and uniformity (P < 0.001) differed between treatments. Steaks treated with C1, C2, C3, and R3 were a brighter red than control steaks. Treatments differed when measuring bone marrow L* (P < 0.001), a* (P < 0.001), and b* (P = 0.004), where R3 treated marrow was the darkest, reddest, and yellowest. Natural antioxidants, specifically acerola cherry powder and rosemary extract, improved steak color and water holding capacity of beef bone-in short ribs aged for an extended period.
40 Novel Genomic Regions Associated with Igg Antibody Response to PRRSV Vaccination Revealed by Haplotype-based GWAS
Authors: Sanglard Leticia P , Huang Yijian , Gray Kent A , Linhares Daniel , Dekkers Jack C , niederwerder Megan , Fernando Rohan , Serão Nick V
Published: May 2021

Previous studies on genomics of antibody response, measured as sample-to-positive (S/P) ratio, to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) have reported a major quantitative trait locus (QTL) on the major histocompatibility complex (MHC) on chromosome 7, explaining ~25% of the genetic variance of this trait. S/P ratio following modified live PRRSV vaccination in crossbred commercial gilts has been proposed as genetic indicator for reproductive performance in non-infected purebred sows and PRRSV-vaccinated crossbred sows. This motivated further genomic study for this trait by performing haplotype-based genome-wide association study (GWAS). 906 naïve F1 (Landrace x Large White) had blood samples taken at ~50d after vaccination for measuring PRRSV ELISA S/P ratio and genotyping. Haplotype-based GWAS identified 8 genomic regions on chromosomes 4 (108 Mb), 7 (15, 21, and 24–27 Mb), and 9 (33 Mb) that were associated (q-value < 0.07) with S/P ratio. From those, only the MHC region (chromosome 7; 24 – 26 Mb) had been identified in the SNP based GWAS. The main SNP identified in the SNP based GWAS (H3GA0020505) was not in LD with the haplotype; thus, we added this SNP to the haplotype model. We observed that the haplotype explained more of the genetic variance compared to the H3GA0020505 SNP, indicating that the MHC haplotype is in stronger LD with the QTL than the H3GA0020505 SNP. All the significant regions associated with S/P ratio included immune-related candidate genes, such as SLA-DOB, TAP2, TAPBP, TMIGD3, and ADORA. This study validated the QTL identified on the MHC region, narrowing the search for causal genes in this region, and identified new genomic regions, along with candidate genes associated with S/P ratio. Identifying novel genomic regions provides more resources for marker-assisted selection and genomic prediction of S/P ratio in purebred and commercial pig populations.
38 Evaluation of Sorghum Phenolic Compounds for Their Antimicrobial Activities Against Liver Abscess Causing Pathogens in Feedlot Cattle
Authors: Salih Harith M , Amachawadi Raghavendra G , Roubicek Cierra , Sexton-Bowser Sarah , Wang Jinrong , Li Yonghui , Nagaraja T G
Published: May 2021

Liver abscesses occur in finishing cattle fed high-grain, low-roughage diets. Cattle with abscessed livers seldom show any clinical signs and are detected only at the time of slaughter. Liver abscesses are of significant economic concern to the feedlot industry. Liver abscesses comprise, on average, 67% of all liver abnormalities in cattle slaughtered in the United States with a prevalence of 10–20% and may reduce the value of the beef carcass up to $38 per animal with the most severe abscesses. There are four causative agents of the disease including the two subspecies of Fusobacterium necrophorum, ssp. necrophorum and ssp. funduliforme, Trueperella pyogenes, and Salmonella enterica. Tylosin, supplemented in the feed, is the most commonly used antibiotic in the feedlot industry to prevent liver abscesses. Because of the concerns about antimicrobial resistance, there is a need to find an effective alternative to this antibiotic, and sorghum grain extracts, which are high in phenolic compounds, may have the potential to be used as natural antibiotic alternatives. Our objectives were to investigate the efficacy of phenolic extracts from black, sumac, brown, and burgundy sorghums on liver abscess pathogens. The sorghum phenolics were extracted using 75% aqueous acetone and total phenolic content was determined by spectrophotometrically. Bacterial strains were cultured in Mueller-Hinton broth (Salmonella and Trueperella pyogenes) or anaerobic brain-heart infusion broth (Fusobacterium) with and without sorghum extracts (1 mg/ml) at 12, 24, and 48 hours and bacterial concentrations were determined. If the compound was inhibitory, a micro-broth dilution method was used to quantify the inhibitory activity. Both black and sumac sorghum phenolics inhibited growth of all four bacterial species. Further studies are ongoing to investigate different concentrations and phenolic compounds from varieties of sorghum grains on the liver abscess pathogens.
33 Comparison of Monensin Sodium Sources for Finishing Beef Cattle
Authors: Husz Taylor C , Smith Wyatt , Lockard Caleb , Homolka Megan , Anderson Pete , Gentry Wes , Sugg Joel D , Jennings Jenny , Casey Kenneth
Published: May 2021

The objective of this study was to evaluate the digestive characteristics of ruminally cannulated beef steers consuming a steam-flaked corn (SFC) or dry-rolled corn (DRC) based diet containing either Rumensin 90 (R) or Monovet 90 (MV). Six ruminally fistulated steers (657.7 kg ± 72.6) housed individually were used in a 6 × 6 Latin square design with a factorial treatment arrangement. Each of the 6 periods were 15 d with 14 d for diet adaptation and 1 d of rumen fluid collections. Six 15-d periods consisted of 14 d diet adaptation prior to rumen fluid collections. Dietary treatments were DRC without monensin sodium (DRC-C), SFC without monensin sodium (SFC-C), DRC with Rumensin 90 (DRC-R), DRC with Monovet 90 (DRC-MV), SFC with Rumensin 90 (SFC-R), and SFC with Monovet 90 (SFC-MV). Rumen contents and fluid were collected through the fistula of each animal at 0, 3, 6, 12, and 24 h on d 15 of each period. Rumen fluid was collected 6 h postfeeding each period for in vitro analyses. Steer was the experimental unit and the model included fixed effects of grain processing, additive, and grain processing × additive. Total gas produced was composited from each in vitro bottle into a gas collection bag for the 48-h determination of methane concentration. No differences were detected for DMI (P = 0.81). Ruminal pH did not differ for the control or additive treatments (P = 0.33). However, ruminal pH was lower (P < 0.01) with SFC relative to DRC There was a significant difference in acetate to propionate ratio for both additive (P = 0.04) and grain type (P ≤ 0.01). Additive inclusion reduced methane proportion of total gas relative to control treatments (P ≤ 0.01). Monensin sodium reduced methane concentration though source had no effect on DMI or ruminal pH.
117 Improved Growth Performance in the F1 Heterozygous Generation of an SSTR2 Knockout Model in Swine
Authors: Schultz R Blythe , Adur Malavika K , Li Yunsheng , Kiefer Zoe E , Gabler Nicholas K , Ross Jason W
Published: May 2021

Targeted genetic alteration provides opportunities for rapid genetic improvement in resilience, welfare and production traits. Somatostatin (SST) acts via negative feedback to regulate growth hormone (GH) activity by antagonizing GH releasing hormone via SST receptors (SSTR) located in the anterior pituitary. Our objective was to reduce the negative effect of SST in the anterior pituitary on protein accretion by reducing the number of functional copies of SSTR2 using the CRISPR/Cas9 system. We hypothesized that a reduction or elimination of SSTR2 would lead to improved growth performance. To test this hypothesis, three commercial gilts were bred with semen from a founder boar in a compound heterozygous state at the SSTR2 locus with a 1bp deletion in one allele resulting in a premature stop codon and a 3bp deletion in the other allele resulting in the loss of a single amino acid predicted to remain functional after translation. Three litters of F1 offspring were produced (n = 46) and all piglets were confirmed to be heterozygous at the SSTR2 locus with one wild type allele and the other possessing either the 1bp deletion (n = 22) or the 3bp (n = 2 4) deletion. No differences (P > 0.43; n=46) in body weight (1.27±0.03 kg) across comparisons were observed at birth. At weaning (n = 36), males (6.19±0.20 kg) were heavier (P = 0.007) than females (5.41±0.19 kg), and piglets possessing a 1bp deletion (6.00±0.20 kg) were numerically heavier (P = 0.14) than 3bp deletion pigs (5.59±0.18 kg). This observation was more pronounced in males at weaning, where the males with the 1bp deletion were 13% heavier (P = 0.058) than those with the 3bp deletion. These data suggest that altering SSTR2 may be a viable genetic advancement strategy to improve growth performance in pigs. This project was supported by the Lloyd L. Anderson Professorship in Physiology at Iowa State University.
114 What Is the Impact of Dairy Influence Cattle on the Traditional Beef Industry Structure?
Authors: Johnson Bradley J , Fuerniss Luke
Published: May 2021

The U.S. cow inventory includes approximately 31 million beef cows and 9 million dairy cows, so flow of cattle from dairies into beef production influences the traditional beef industry structure. Dairy-influenced cattle have historically entered the beef supply chain as cull cows and calf-fed Holstein steers. Culled dairy cows account for approximately half of the cows harvested in the United States annually. Fed steers and heifers of dairy influence are estimated to account for 15% of annual steer and heifer slaughter. Advancements in data availability, genomics, and reproductive technologies have enabled more precise selection of dairy replacement heifers and more pregnancies to be allocated to a terminal sire. Recently, the use of beef semen to breed dairy cows that are not desirable for producing replacement heifers has become more widespread. Beef-on-dairy calves are often moved to calf ranches shortly after birth where they are weaned and grown before transitioning to traditional grow yards or feedlots. In comparison to traditional range beef production, calves of dairy origin are weaned at a younger age, have more restricted mobility early in life, and are fed a delivered ration for a greater number of days. While carcasses of dairy-originated fed cattle excel in subcutaneous leanness and marbling, calves originating from dairies typically experience greater morbidity, poorer feed conversion, and poorer dressed yields compared to native fed cattle. Future opportunities to optimize beef production from the dairy herd include refining sire selection to consistently produce high quality calves, reducing variation in calfhood management, and identifying optimal nutrition and growth technology programs for calves from dairies.
112 How Will the Purebred Association Adapt to a Changing Beef Industry?
Authors: McCully Mark
Published: May 2021

The role of the breed association has historically been to keep a registry of a pure breed, aggregate the performance data surrounding that one breed, develop breeding and selection tools, and conduct breed promotion. Larger associations have been able to augment that with operating magazines and other media, running branded beef programs, feeder calf marketing programs, and genetic evaluation for other breeds. The relevance of breed associations is being and will continue to be challenged as genomics and large commercial databases develop and allow for breeding and selection tools to be developed independently by large breeders or private entities. Gene editing and other such technology will also challenge the traditional seedstock models and opens the door for proprietary genetic lines. Breed associations may need to modify their traditional policies to incorporate these innovations. Supply chains will continue to become far more sophisticated and will incorporate more genetic information to guide management decisions and potentially validate brand promises around sustainability. To stay relevant, breed associations of the future will need to do the following:

Balance the needs of diverse membership (show, hobby, lifestyle, etc.) with commercial industry value and significance. Have access to large amounts of data and be leaders in adopting the most current technologies. Deliver tools for breeders that enhance the profitability of commercial producers – identify optimum production levels vs maximum outputs. Work collaboratively with multiple supply chains providing the needed genetic information. Be a significant educational resource to breeders and commercial producers. Be a leader in research on breed improvement and genetic advancement. Have value-added programs that create real and sustained pull-through demand for the end product.
110 The Importance of Recognizing Economic Factors Influencing the Beef Industry
Authors: Rishel Bill
Published: May 2021

The speed of development and adaptation of genomics in our industry as a tool for genetic improvement has been unprecedented. While its greatest contribution has been the enhancement of the predictive accuracy of performance traits, even greater opportunities may exist for the application of genomics in the arena of animal health. Interactions between immune system function and the microbiome is mission critical for an industry that may face increased pressure to find new animal health protocols for animal care. Better understanding of the incorporation of dry matter intake and residual average daily gain EPD’s into decision making will provide greater incentives to improve efficiency. Understanding the economic impact of incremental change in a EPD will aid decision making. A predictive blueprint of genomic characteristics of feeder cattle will allow producers and feed yards to merchandise feeder and fed cattle to specific markets. Greater connection of the beef industry across multiple segments will result in better understanding of the whole beef system. Increasing value within one segment can ultimately result in greater value across multiple segments and true price discovery. Understanding the important relationship of cattle to the environment and society will become increasingly important to maintain sustainable beef production. The natural and unique ability the beef cow possesses to utilize grass resources on lands not fit for cultivation and upcycle those resources to a nutrient dense food for human nutrition is unequaled in nature. This process is sustainable, totally regenerative and beneficial to the environment and society. Beef production is a valuable asset to both public and private grasslands and sustainability of our natural resources. Maintaining grazing on federal lands is good resource management for a viable beef industry. Opportunities exist for greater collaborations between producers, academia, breed associations and national organizations to solve problems and provide better educational opportunities.
113 Genetics Industry Infrastructure: How Are Artificial Insemination Organizations Adapting to Changes in the Beef Industry?
Authors: Harstine Bo , Marshall Lorna , DeJarnette Mel
Published: May 2021

Cattle artificial insemination (AI) organizations are adapting quickly to changes within the beef industry domestically and abroad. Specifically, the increasing utilization of beef bulls, predominately Angus, in dairy herds to create terminal crossbred calves for beef underlies the majority of the changes and challenges. The procurement, management, and collection of beef sires on a commercial scale has become commonplace for AI organizations, and the subsequent optimization of beef sire housing, nutritional requirements, behavior surrounding semen collection, semen production ability, semen quality, and fertility is being examined. At one large AI organization, Angus sires are proving capable of producing as much semen as their dairy counterparts while being collected year-round to meet market demand. However, the percentage of collections that qualify for sale is ≤80% in beef bulls compared to >90% in Holstein bulls based on acceptable semen quality as determined by post-thaw computer-assisted motility analyses, flow cytometric viability assays, and visual sperm morphological assessment (Select Sires, Inc., unpublished). Large numbers of inseminations using beef sires, combined with reliable data recording on dairies, is allowing for precision management tools and sire fertility estimates to be generated. For example, the Council on Dairy Cattle Breeding (CDCB) recently applied the Sire Conception Rate (SCR) evaluation’s statistical model to Angus bulls used to inseminate Holstein cows and reported similar conception rates to Holstein sires used to inseminate Holstein cows (33.8% versus 34.3%, respectively). AI organizations have also begun to implement research, create products, and establish new supply chains to proactively account for dairy farms supplying crossbred or purebred (via embryo transfer) beef animals to calf ranches, feed yards, and packer networks. Examples include purebred commercial beef embryo sales, research on rearing crossbred beef calves, generating novel value-based (grid pricing) markets for crossbred calves, and facilitating traceability programs for packers.