bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vivo Efficacy of a Nanoconjugated Glycopeptide Antibiotic in Silkworm Larvae Infected by Staphylococcus aureus
Authors: Montali Aurora , Berini Francesca , Gamberoni Federica , Armenia Ilaria , Saviane Alessio , Cappellozza Silvia , Gornati Rosalba , Bernardini Giovanni , Marinelli Flavia , Tettamanti Gianluca
Published: November 2024

To contrast the rapid spread of antibiotic resistance in bacteria, new alternative therapeutic options are urgently needed. The use of nanoparticles as carriers for clinically relevant antibiotics represents a promising solution to potentiate their efficacy. In this study, we used Bombyx mori larvae for the first time as an animal model for testing a nanoconjugated glycopeptide antibiotic (teicoplanin) against Staphylococcus aureus infection. B. mori larvae might thus replace the use of mammalian models for preclinical tests, in agreement with the European Parliament Directive 2010/63/EU. The curative effect of teicoplanin (a last resort antibiotic against Gram-positive bacterial pathogens) conjugated to iron oxide nanoparticles was assessed by monitoring the survival rate of the larvae and some immunological markers (i.e., hemocyte viability, phenoloxidase system activation, and lysozyme activity). Human physiological conditions of infection were reproduced by performing the experiments at 37 °C. In this condition, nanoconjugated teicoplanin cured the bacterial infection at the same antibiotic concentration of the free counterpart, blocking the insect immune response without causing mortality of silkworm larvae. These results demonstrate the value and robustness of the silkworm as an infection model for testing the in vivo efficacy of nanoconjugated antimicrobial molecules.
Hemadsorption with CytoSorb in Infants with Sepsis: Non-Systematic Review of Cases
Authors: Borankulova Aruzhan , Sazonov Vitaliy
Published: November 2024

Sepsis is a severe and potentially life-threatening condition that occurs when the body’s response to an infection damages its own tissues and organs. It can lead to organ failure and death if not treated. Cytosorb is a promising medical device for hemadsorption in pediatric septic patients (under 18 years old). As many studies conclude, Cytosorb results in efficient hemodynamics stabilization. This paper is a nonsystematic review of cases. PubMed-, Google Scholar-, and Scopus-indexed journals were used to collect papers for the research. Overall, 11 pediatric cases (six journal articles) were collected. Reductions in interleukin (IL)-6 and IL-10 levels after hemoperfusion with CytoSorb suggest a potential benefit in modulating the inflammatory response in pediatric patients. Moreover, other septic shock indicators such as C-reactive protein, lactate, procalcitonin, ALT, and AST were also significantly reduced in surviving patients within the first few hours of hemadsorption with CytoSorb. The use of CytoSorb seems to be efficient in managing different sepsis-related conditions, even in neonatal and infant populations, as a valuable supplementary tool. However, overcoming the obstacles associated with the age and weight of pediatric patients might necessitate the creation of CytoSorb devices tailored specifically to their needs.
Digitization of Dentate and Edentulous Maxillectomy and Mandibulectomy Defects with Three Different Intraoral Scanners: A Comparative In Vitro Study
Authors: Hattori Mariko , Stadler Sandra , Sumita Yuka I. , Spies Benedikt C. , Vach Kirstin , Kohal Ralf-Joachim , Wakabayashi Noriyuki
Published: November 2024

Objectives: The objective of this study was to compare the trueness and precision of three intraoral scanners (IOSs) for the digitization of dentate and edentulous maxillectomy and mandibulectomy defects in artificial models. Methods: Four representative defect models—a dentate and an edentulous maxillectomy model and a dentate and an edentulous mandibulectomy model—were used for digital scanning. After a reference scan of each model, they were scanned with three IOSs: CEREC AC Omnicam, True Definition, and cara TRIOS 3. For comparison, five conventional impressions with a polysiloxane material were taken and digitized with a laboratory scanner. The obtained data were evaluated with three-dimensional (3D) inspection software and superimposed with the reference scan data by using a best-fit algorithm. The mean absolute 3D deviations of the IOS compared to the reference data (trueness) and when comparing the datasets within the IOS (precision) were analyzed. Linear mixed models and multiple pairwise comparisons were used for statistical analyses. Results: The overall comparison of the four evaluated procedures for data acquisition showed a significant difference in trueness (p < 0.0001) and precision (p < 0.0001). The average mean trueness of the IOSs ranged from 32.17 to 204.43 µm, compared to 32.07 to 64.85 µm for conventional impressions. Here, the conventional impression and cara TRIOS 3 performed the most precisely with no significant difference. CEREC AC Omnicam achieved the worst precision. Conclusions: Using a suitable intraoral scanner, defective jaws even without teeth could be captured in satisfying accuracy. This shows the possibility to use an intraoral scanner for maxillofacial defect patients and gives a vision of using digital technology in maxillofacial prosthetics.
Chronic Spontaneous Urticaria—New Predictor on the Horizon?
Authors: Bartosik Bartosz , Kapeluszna Katarzyna , Bartosik Dagmara , Chobot Agata , Ciszewska-Hołda Paulina , Gawrylak-Dryja Ewa , Klus Anna , Bułdak Rafał , Brzoza Zenon
Published: November 2024

Chronic urticaria is one of the most common diseases in allergology and dermatology practice with unclear causes of occurrence. Background: Some studies emphasize the correlation between inflammation in chronic urticaria and disturbed intestinal microbiota. It raises the question about the role of some intestine-related substances in the pathogenesis of urticaria as well as their potential role as disease predictors. Calprotectin is an acute-phase protein with a well-established diagnostic position in the field of gastroenterology. There are some data on the relationship between this parameter and gut microbiota. The major aim of this preliminary study is to investigate whether calprotectin can be potentially taken into account as a disease course predictor in urticaria. Methods: We included in our study 54 chronic spontaneous urticaria (CSU) patients (of whom 26 manifested the symptoms of recurrent angioedema) and 29 patients allergic to Hymenoptera venom for the reference group (in these patients, before venom immunotherapy induction, full diagnostics is performed including intestinal problems). Disease activity in the CSU patients was assessed using the Urticaria Activity Score (UAS) and the disease control in this group was assessed with the Urticaria Control Test (UCT). Moreover, we analyzed fecal and serum calprotectin concentrations. Results: Positive correlation was found only between the values of serum calprotectin concentration and the control level of CSU symptoms with the lack of other relations. Conclusions: Our results do not supply unequivocal evidence for calprotectin as a potential marker of CSU course, though this concept, in the light of growing evidence for microbiota’s role in urticaria, requires further research.
Therapeutic Strategies for Retinal Artery Occlusion—A Literature Review
Authors: Roskal-Wałek Joanna , Ruzik Alicja , Kubiś Natalia , Teper Maria , Wesołowski Michał , Wujec Zuzanna , Wałek Paweł , Odrobina Dominik , Mackiewicz Jerzy , Wożakowska-Kapłon Beata
Published: November 2024

Retinal artery occlusion (RAO) is an emergency condition causing acute retinal ischemia and is considered an equivalent of ischemic stroke. The occurrence of an episode of RAO is associated with significant impairment of visual functions and correlates with an increased risk of future vascular events. Although RAO requires immediate diagnosis and treatment, there are currently no clear guidelines specifying optimal management. This review discusses current and future therapeutic strategies following an episode of RAO, including secondary prevention.
Relationship Between Obstructive Sleep Apnea and Sports—Systematic Review and Meta-Analysis
Authors: Martínez Revuelta Lucía , Flores-Fraile Javier , Zubizarreta-Macho Álvaro , Montiel-Company José María , Lobo-Galindo Ana Belén , Arrieta Blanco Patricia
Published: November 2024

The primary objective of this research is to analyze and compare the effects of physical activity on patients diagnosed with obstructive sleep apnea (OSA), a condition characterized by repeated episodes of partial or complete obstruction of the upper airway during sleep, leading to disrupted sleep and various health complications. Methods: A systematic and strategic search of 16 peer-reviewed articles was conducted to assess the impact of different types of physical exercise on the apnea–hypopnea index (AHI), a key metric used to quantify the severity of OSA. The selected studies focused on two main exercise modalities: aerobic exercise alone and a combination of aerobic and resistance training. These modalities were examined to determine their respective influences on the AHI among individuals with OSA. Results: The findings revealed that aerobic exercise alone did not significantly alter the apnea–hypopnea index (p = 0.15), suggesting limited effectiveness in reducing the severity of OSA when performed in isolation. However, in patients who engaged in combined physical activity—incorporating both aerobic and resistance exercises—a significant reduction in the AHI was observed (p < 0.01), indicating a more substantial therapeutic effect. Conclusions: This study concludes that a structured exercise program combining aerobic and resistance training produces a significant improvement in the apnea–hypopnea indices of patients with obstructive sleep apnea. These results highlight the potential of combined exercise regimens as a non-pharmacological intervention that could complement traditional OSA treatments, offering a viable approach to managing the condition and improving patient outcomes. Future research should aim to explore the optimal exercise intensity, duration, and frequency to maximize these benefits.
Impact of Interhospital Transfer on Outcomes in Acute Pancreatitis: Implications for Healthcare Quality
Authors: Kahan Tamara F. , Manoj Matthew Antony , Chhoda Ankit , Liyen Cartelle Anabel , Anderson Kelsey , Zuberi Shaharyar A. , Freedman Steven D. , Sheth Sunil G.
Published: November 2024

Background/Objectives: Effective management of acute pancreatitis (AP) hinges on prompt volume resuscitation and is adversely affected by delays in diagnosis. Given diverse clinical settings (tertiary care vs. community hospitals), further investigation is needed to understand the impact of the initial setting to which patients presented on clinical outcomes and quality of care. This study aimed to compare outcomes and quality indicators between AP patients who first presented to the emergency department (ED) of a tertiary care center and AP patients transferred from community hospitals. Methods: This study included AP patients managed at our tertiary care hospital between 2008 and 2018. We compared demographics and outcomes, including length of stay (LOS), intensive care unit (ICU) admission, rates of local and systemic complications, re-admission rates, and one-year mortality in transferred patients and those admitted from the ED. Quality indicators of interest included duration of volume resuscitation, time until advancement to enteral feeding, pain requiring opioid medication [measured in morphine milliequivalent (MME) dosing], and surgical referrals for cholecystectomy. Categorical variables were analyzed by chi-square or Fisher’s exact test; continuous variables were compared using Kruskal–Wallis tests. Regression was performed to assess the impact of transfer status on our outcomes of interest. Results: Our cohort of 882 AP patients comprised 648 patients admitted from the ED and 234 patients transferred from a community hospital. Transferred patients were older (54.6 vs. 51.0 years old, p < 0.01) and had less frequent alcohol use (28% vs. 39%, p < 0.01). Transferred patients had a significantly greater frequency of gallstone AP (40% vs. 23%), but a lower frequency of alcohol AP (16% vs. 22%) and idiopathic AP (29% vs. 41%) (p < 0.001). Regarding clinical outcomes, transferred patients had significantly higher rates of severe AP (revised Atlanta classification) (10% vs. 2% severe, p < 0.001) and ICU admission (8% vs. 2%, p < 0.001) and longer median LOS (5 vs. 4 days, p < 0.001). Regarding quality indicators, there was no significant difference in the number of days of intravenous fluid administration, or days until advancement to enteral feeding, pain requiring opioid pain medication, or rates of surgical referral for cholecystectomy. Conclusions: Though the quality of care was similar in both groups, transferred patients had more severe AP with higher rates of systemic complications and ICU admissions and longer LOS, with no difference in quality indicators between groups.
Two-Year Experience of a Center of Excellence for the Comprehensive Management of Non-Small Cell Lung Cancer at a Fourth-Level Hospital in Bogota, Colombia: Observational Case Series Study and Retrospective Analysis
Authors: García-Herreros Luis Gerardo , Rico-Rivera Enid Ximena , García Morales Olga Milena
Published: November 2024

Background: This study aimed to provide a comprehensive analysis of 56 patients admitted to the Lung Cancer Clinical Care Center (C3) at Fundación Santa Fe de Bogotá (FSFB) between 2 May 2022 and 22 April 2024. The focus was on demographic characteristics, smoking history, comorbidities, lung cancer types, TNM classification, treatment modalities, and outcomes. Methods: This observational case series study reviewed medical records and included patients over 18 years with a confirmed diagnosis of non-small cell lung cancer (NSCLC). Data were collected and analyzed for demographics, comorbidities, treatment types, biomolecular profiling, and survival rates. Ethical approval was obtained, and data were anonymized. Results: The mean age was 71.8 years with a female predominance (53.6%). A history of smoking was present in 71.4% of patients. Adenocarcinoma was the most common type (75.0%), followed by squamous cell carcinoma (19.6%). At admission, the most frequent TNM stages were IA2 (17.9%) and IVA (16.1%). One-year survival was 68.8%, and 94.3% of stage I–IIIA patients underwent PET scans. Biomolecular profiling revealed 69.2% non-mutated EGFR, 90.4% ALK-negative, and various PDL-1 expression levels. Immunotherapy was received by 91.4% of patients, with Alectinib and Osimertinib being common. Grade III–IV pneumonitis occurred in 5.4% of patients. Conclusions: The study’s findings align with existing literature, highlighting significant smoking history, common adenocarcinoma, and substantial use of immunotherapy. Limitations include the observational design, small sample size, and short follow-up period, impacting the generalizability and long-term outcome assessment. Future research should address these limitations and explore longitudinal outcomes and emerging therapies.
Could Hepcidin Be a New Biomarker in Patients with Idiopathic Pulmonary Fibrosis (IPF)?
Authors: Yilmaz Gulcin , Çoban Hikmet , Sarioglu Nurhan , Erel Fuat , Yılmaz Merve Akış , Çolak Mustafa , Yumrukuz Şenel Merve , Hismioğulları Adnan Adil
Published: November 2024

Objectives: Hepcidin is a biomarker produced by hepatocytes in chronic disease anemia and is known to increase during chronic inflammation. This study compares the hepcidin levels in idiopathic pulmonary fibrosis (IPF) patients and controls, evaluating its relationship with anemia and systemic inflammation in IPF patients. Methods: This study included 82 IPF patients and 31 controls. Hepcidin levels were compared between the two groups. In the IPF group, the hepcidin and anemia parameters were compared between anemic and non-anemic patients. The significance between the hepcidin and systemic inflammation parameters such as Erythrocyte Sedimentation Rate, CRP (C-reactive protein) levels, ferritin levels, and the Systemic Immune–Inflammation Index (SII) was investigated. Erythrocyte Sedimentation Rate, C-reactive protein (CRP) levels, and ferritin levels were measured using automated analyzers. Hepcidin and erythropoietin (EPO) levels were determined using ELISA kits. Results: A significant difference in hepcidin levels was found between the IPF and control groups (37.13 ± 14.92 vs. 25.77 ± 11.25, p < 0.001). No significant difference in hepcidin levels was found between anemic and non-anemic IPF patients (38.25 ± 16.2 vs. 36.7 ± 14.6, p = 0.719). No significant correlation was found between hepcidin levels and anemia parameters (serum iron, ferritin, vitamin B12, serum transferrin, transferrin saturation, total iron-binding capacity, hemoglobin, folate, and erythropoietin) in IPF patients. Despite significant differences in the systemic inflammation parameters (ferritin and CRP) between patients and controls, no significant correlation was found between their hepcidin and systemic inflammation parameters. Conclusions: Our study demonstrates that the hepcidin levels in IPF patients are elevated independently of anemia and systemic inflammation. We propose that hepcidin could be a potential biomarker to be investigated in IPF patients.
Beyond the Hip: Clinical Phenotypes of Hip Osteoarthritis Across the Biopsychosocial Spectrum
Authors: Sergooris Abner , Verbrugghe Jonas , Bonnechère Bruno , Klaps Sim , Matheve Thomas , Vandeputte Frans-Jozef , Corten Kristoff , Bogaerts Katleen , Timmermans Annick
Published: November 2024

Background/Objectives: To identify clinical phenotypes of hip osteoarthritis (OA) within a biopsychosocial framework. Methods: A cross-sectional analysis of 143 individuals with hip OA awaiting total hip arthroplasty (THA) was performed. Phenotyping features included sociodemographic and biomedical information, pain-related cognitions and emotions, mental disorders, traumatic experiences, self-efficacy, social support, perceived stress, and somatosensory function. Outcome measures included the hip disability and osteoarthritis outcome score and the numeric pain-rating scale. Decision tree learning was used to select the most important phenotyping features. K-means clustering analyses were performed to identify clinical phenotypes and a decision tree algorithm was trained to classify individuals in the identified clinical phenotypes. Results: Selected phenotyping features associated with pain and disability included a combination of biomedical, psychological, and social variables. Two distinct clinical phenotypes were identified. Individuals within the maladaptive phenotype (34%) reported more comorbidities, less self-efficacy and higher levels of anxiety, depression, pain-related fear-avoidance, and feelings of injustice. No differences were found regarding social support and somatosensory function. Regarding the outcome measures, individuals within the maladaptive phenotype reported higher levels of pain and disability. Finally, based on the Fear-Avoidance Components Scale (FACS) and the anxiety subscale of the Hospital Anxiety and Depression Scale (HADS-A), individuals could be classified into the clinical phenotypes with 87.8% accuracy. Conclusions: Two clinical phenotypes, an adaptive and a maladaptive phenotype, can be identified in individuals with hip OA using the FACS and HADS-A. The identification of these clinical phenotypes represents a crucial step toward precision medicine, enabling the development of targeted treatment pathways tailored to the distinct biomedical and psychological features of each phenotype.
MRI Spinal Cord Reconstruction Provides Insights into Mapping and Migration Following Percutaneous Epidural Stimulation Implantation in Spinal Cord Injury
Authors: Venigalla Siddharth , Rehman Muhammad Uzair , Deitrich Jakob N. , Trainer Robert , Gorgey Ashraf S.
Published: November 2024

Background: Spinal cord epidural stimulation (SCES) has the potential to restore motor functions following spinal cord injury (SCI). Spinal cord mapping is a cornerstone step towards successfully configuring SCES to improve motor function, aiming to restore standing and stepping abilities in individuals with SCI. While some centers have advocated for the use of intraoperative mapping to anatomically target the spinal cord locomotor centers, this is a resource-intensive endeavor and may not be a feasible approach in all centers. Methods: Two participants underwent percutaneous SCES implantation as part of a clinical trial. Each participant underwent a temporary (1-week, two-lead) trial followed by a permanent, two-lead implantation. SCES configurations were matched between temporary and permanent mappings, and motor evoked potential in response to 2 Hz, for a duration of 250–1000 µs and with an amplitude of 1–14 mA, was measured using electromyography. T2 axial MRI images captured prior to implantation were used to retrospectively reconstruct the lumbosacral segments of the spinal cord. The effects of lead migration on mapping were further determined in one of the participants. Results: In both participants, there were recognized discrepancies in the recruitment curves of the motor evoked potentials across different muscle groups between temporary and permanent SCES mappings. These may be explained by retrospective MRI reconstruction of the spinal cord, which indicated that the percutaneous leads did not specifically target the entire L1-S2 segments in both participants. Minor lead migration appeared to have a minimal impact on spinal cord mapping outcomes in one of the participants but did dampen the motor activity of the hip and knee muscle groups. Conclusions: Temporary mapping coupled with MRI reconstruction has the potential to be considered as guidance for permanent implantation considering target activation of the spinal cord locomotor centers. Since lead migration may alter the synergistic coordination between different muscle groups and since lead migration of 1–2 contacts is expected and planned for in clinical practice, it can be better guided with proper spinal cord mapping and a diligent SCES lead trial beforehand.
Neutrophils Respond Selectively to Physical Cues: Roughness Modulates Its Granule Release, and NETosis
Authors: Elangovan Gayathiri , Fernandes Daniel J. , Cameron Andrew , Basu Souptik , De Mello Neto Joao Martins , Jiang Peishan , Reher Peter , Hamlet Stephen , Figueredo Carlos Marcelo S.
Published: November 2024

Our study examined how different titanium alloy Ti6Al4V (Ti64) and zirconia (ZrO2) surfaces, ranging from rough to very smooth, affect the expression of elastase (NE), matrix metalloproteinase (MMP)-8, MMP-9, and extracellular traps (NETs) by neutrophils. Discs of Ti64 and ZrO2, 10 mm in diameter and 1.5 mm thick, were created using diamond-impregnated polishing burs and paste to produce rough (Ra > 3 µm), smooth (Ra ≥ 1 to 1.5 µm), and very smooth (Ra < 0.1 µm) surfaces. Neutrophils from Wistar rats were cultured on these surfaces, and the culture supernatants were then examined for NE, MMP-8, and MMP-9 using ELISA. At the same time, NET formation was demonstrated immunohistochemically by staining neutrophils with CD16b and DNA with DAPI. Overall, the expressions of NE and MMP-8 were significantly higher from neutrophil culture on Ti64 and ZrO2 rough surfaces compared to the very smooth surface (R > S > VS) after 2 h and 4 h of culture. The expression of MMP-9 also increased with culture time; however, no significant surface effects on expression were observed. Similarly, rough Ti64 and ZrO2 surfaces (R & S) also showed significantly larger NET formation compared to the very smooth surface (VS) after 4 h and 8 h cultures. Our findings suggest that increasing surface roughness on Ti64 and ZrO2 triggers higher NE, MMP-8, and NET formation secretion.
Pattern Recognition Receptors in Periodontal Disease: Molecular Mechanisms, Signaling Pathways, and Therapeutic Implications
Authors: Ferrara Elisabetta , Mastrocola Francesco
Published: November 2024

Periodontal disease remains a significant global health concern, characterized by complex host–pathogen interactions leading to tissue destruction. This review explored the role of pattern recognition receptors (PRRs) in the pathogenesis of periodontal disease, synthesizing current knowledge on their molecular mechanisms and potential as therapeutic targets. We examined the diverse family of PRRs, focusing on toll-like receptors (TLRs) and NOD-like receptors (NLRs), elucidating their activation by periodontal pathogens and subsequent downstream signaling cascades. This review highlights the intricate interplay between PRR-mediated pathways, including NF-κB and MAPK signaling, and their impact on inflammatory responses and bone metabolism in periodontal tissues. We discussed the emerging concept of PRR crosstalk and its implications for periodontal homeostasis and disease progression. Furthermore, this review addressed the potential of PRR-targeted therapies, exploring both challenges and opportunities in translating molecular insights into clinical applications. By providing an overview of PRRs in periodontal health and disease, this review aims to stimulate future research directions and inform the development of novel diagnostic and therapeutic strategies in periodontology.
An Analytical Study of Tsunamis Generated by Submarine Landslides
Authors: Uribe Francisco , Fuentes Mauricio , Campos Jaime
Published: November 2024

In this work, the problem of tsunamis generated by underwater landslides is addressed. Two new solutions are derived in the framework of the linear shallow water equations and linear potential wave theory, respectively. Those solutions are analytical (1 + 1D) and another is semi-analytical (2 + 1D). The 1 + 1D model considers a solid body sliding over a sloping beach at a constant speed, and the 2 + 1D model considers a solid landslide that moves at a constant velocity on a flat bottom. The solution 1 + 1D is checked numerically using a different finite scheme. The 2 + 1D model examines the kinematic and geometric features of the landslide at a constant ocean depth and its influence on the generation of tsunamis. Landslide geometry significantly influences run-up height. Our results reveal a power law relationship between normalized run-up and landslide velocity within a realistic range and a negative power law for the landslide length–thickness. Additionally, a critical aspect ratio between the length and width of the sliding body is identified, which enhances the tsunamigenic process. Finally, the results show that the landslide shape does not have a decisive influence on the pattern of tsunami wave generation and propagation.
Research on Effect of Ship Speed on Unsteady Hydrodynamic Performance of Bow Thrusters in Berthing and Departure Directions
Authors: Cai He , Ma Xiaoqian , Wen Tan , Sun Yu , Yang Zhiyuan , Tan Yilong , Zhuo Jianyu
Published: November 2024

With the continuous development of the shipping market, bow thrusters have become more important for ship maneuvering. Therefore, the performance of bow thrusters is studied in this paper. In order to obtain an unsteady performance of the bow thruster under different ship speed conditions, the SST k-ω turbulence model is adopted to predict the hydrodynamics of the bow thruster. With the ship’s speed increasing gradually, the variation characteristics of hydrodynamic coefficients and the flow field distribution at key positions are analyzed. The results show that with an increase in ship speed to three knots, the thrust coefficient and torque coefficient of the bow thruster decrease by 2.69~4.07% and 2.34~3.08%. In addition, the blade vibration amplitude intensifies. In the departure direction, the propeller load is more susceptible to being influenced and decreases by an additional 2.34~4.16% compared with that in the berthing direction. Meanwhile, it is found that the velocity distribution is asymmetrical. The inlet velocity at the bow side is faster, which results in the maximum peak pressure being about three times the minimum peak pressure. In addition, the pressure’s nonuniformity in the tunnel increases gradually with the increase in ship speed. Compared with the pressure distribution in the berthing direction, the pressure distribution before and after the propeller is more uniform, which is consistent with the results of hydrodynamic change and velocity distribution. The research in this paper has a certain reference significance for understanding the hydrodynamic performance of bow thrust operation.
Study on Spatial Scale Selection Problem: Taking Port Spatial Expression as Example
Authors: Xu Yunzhuo , Xu Xinglu , Wang Wenyuan , Guo Zijian
Published: November 2024

Spatial scale is a key factor, which affects the accuracy of spatial expression and further influences the spatial planning of a research area. In order to help improve the efficiency and accuracy of optimal scale selection for all sizes of research areas, a universal two-layer theoretical framework for optimal scale selection was proposed in this paper. Port area was taken as an example to systematically clarify the application of the proposed framework, and the scale selection model for port spatial expression was established. Least-squares-based mean change point analysis was introduced into the model, and the concept of a comprehensive change point was proposed to form the criterion for optimal port scale selection. First, an appropriate scale domain was preliminarily determined by the upper scale selection model. Then, the lower scale selection model determined the final appropriate scale domain and took the corresponding scale of a minimum comprehensive change point as the final optimal scale for port spatial expression. Finally, a port area in Qingdao in eastern China was taken to verify the feasibility of the proposed model, and the optimal scale was suggested to be 14 m. The proposed framework in this paper helps ensure the accuracy of spatial expression and reduce spatial data redundancy, and it can provide the methodological references for planners to better spatialize a research area, which will guarantee the subsequent spatial planning work.
Molecular Biodiversity in Fusarium subglutinans and F. temperatum: A Valuable Tool to Distinguish the Two Sister Species and Determine the Beauvericin Chemotype
Authors: Susca Antonia , Villani Alessandra , Haidukowski Miriam , Epifani Filomena , Logrieco Antonio F. , Moretti Antonio
Published: November 2024

Fusarium subglutinans and F. temperatum are widely distributed maize pathogens recognized as distinct species with a species-specific chemotype based on patterns of mycotoxins. Recent comparative genomic analysis revealed that genomes of both species carry a complete beauvericin (Bea) biosynthetic genes cluster, but the key gene Bea1 in F. subglutinans is not functional likely due to a large insertion (NRPS22ins) and multiple mutations (SNP298 and SNP528). We used the recently published genome sequences for these species to develop PCR markers for investigating the distribution of three main mutations in the Bea1 gene in a large collection of strains of both species from around the world. We also designed a PCR assay for a rapid and reliable discrimination of both species in the evaluation of crop exposure to mycotoxins. Overall, our results showed that SNP528 was the most common mutation, followed by NRPS22ins and SNP298. Moreover, phylogenetic analyses suggest that non-synonymous SNPs have occurred first, and that the resulting inactivation of BEA production has caused the accumulation of other polymorphisms, including the NRPS22ins, in the entire gene-coding region. The screening for genetic differences between these species could guide future crop management strategies.
Roles of Different Signaling Pathways in Cryptococcus neoformans Virulence
Authors: Mahmood Fawad , Chen Jun-Ming , Al-Huthaifi Ammar Mutahar , Al-Alawi Abdullah Ali , Liu Tong-Bao
Published: November 2024

Cryptococcus neoformans is a widespread fungal pathogen that can infect the human central nervous system (CNS) and cause fungal meningitis, leading to hundreds of thousands of deaths worldwide each year. Previous studies have demonstrated that many signal transduction pathways are crucial for the morphological development and virulence of C. neoformans. In this review, data from over 116 research articles have been compiled to show that many signaling pathways control various characteristics of C. neoformans, individually or in association with other pathways, and to establish strong links among them to better understand C. neoformans pathogenesis. Every characteristic of C. neoformans is closely linked to these signaling pathways, making this a rich area for further research. It is essential to thoroughly explore these pathways to address questions that remain and apply a molecular mechanistic approach to link them. Targeting these pathways is crucial for understanding the exact mechanism of infection pathogenesis and will facilitate the development of antifungal drugs as well as the diagnosis and prevention of cryptococcosis.
The Effects of COVID-19 on Antifungal Prescribing in the UK—Lessons to Learn
Authors: Pates Katharine , Shang Zhendan , Jabbar Rebeka , Armstrong-James Darius , Schelenz Silke , Periselneris Jimstan , Arcucci Rossella , Shah Anand
Published: November 2024

Fungal infections are increasingly prevalent; however, antifungal stewardship attracts little funding or attention. Previous studies have shown that knowledge of guidelines and scientific evidence regarding antifungals is poor, leading to prescribing based on personal experiences and the inherent biases this entails. We carried out a retrospective study of inpatient antifungal usage at two major hospitals. We assessed the longitudinal trends in antifungal usage and the effect of COVID-19 on antifungal prescription, alongside levels of empirical and diagnostically targeted antifungal usage. Our results showed that the longitudinal patterns of total systemic antifungal usage within the trusts were similar to national prescribing trends; however, the composition of antifungals varied considerably, even when looking exclusively at the more homogenous group of COVID-19 patients. We showed a high level of empirical antifungal use in COVID-19 patients, with neither trust adhering to international recommendations and instead appearing to follow prior prescribing habits. This study highlights the significant challenges to optimise antifungal use with prescribing behaviour largely dictated by habit, a lack of adherence to guidelines, and high rates of empirical non-diagnostic-based prescribing. Further research and resources are required to understand the impact of antifungal stewardship on improving antifungal prescribing behaviours in this setting and the effects on outcome.
Invasive Aspergillosis with Cavernous Sinus Thrombosis Following High-Dose Corticosteroid Therapy: A Challenging Case of Rhino-Orbital-Cerebral Mycosis
Authors: Karakeçili Faruk , Barkay Orçun , Sümer Betül , Binay Umut Devrim , Memiş Kemal Buğra , Yapıcıer Özlem , Balcı Mecdi Gürhan
Published: November 2024

Invasive aspergillosis is a rare but severe fungal infection primarily affecting immunocompromised individuals. The Coronavirus Disease-2019 (COVID-19) pandemic has introduced new complexities in managing aspergillosis due to the widespread use of corticosteroids for treating COVID-19-related respiratory distress, which can increase susceptibility to fungal infections. Here, we present a challenging case of progressive cerebral aspergillosis complicated by cavernous sinus thrombosis (CST) in a 67-year-old male with a history of COVID-19. The patient, initially misdiagnosed with temporal arteritis, received pulse corticosteroid therapy twice before presenting with persistent left-sided headaches and vision loss. Cranial imaging revealed findings consistent with fungal sinusitis, Tolosa–Hunt syndrome, and orbital pseudotumor, which progressed despite initial antifungal therapy. Subsequent magnetic resonance imaging indicated an invasive mass extending into the left cavernous sinus and other intracranial structures, raising suspicion of aspergillosis. A transsphenoidal biopsy confirmed Aspergillus infection, leading to voriconazole therapy. Despite aggressive treatment, follow-up imaging revealed significant progression, with extension to the right frontal region and left cavernous sinus. The patient then developed visual impairment in the right eye and was diagnosed with CST secondary to fungal sinusitis. Management included a combination of systemic antifungals and antibiotics; however, the patient declined surgical intervention. This case underscores the diagnostic challenges and rapid progression associated with cerebral aspergillosis in post-COVID-19 patients treated with corticosteroids. This report highlights the need for heightened clinical suspicion and prompt, targeted interventions in similar cases to improve patient outcomes. Further research is required to understand the optimal management of invasive fungal infections.
Associations Between Plasma Levels of NLRP3 Protein, Interleukin-1 Beta and Features of Acute ST-Elevation Myocardial Infarction
Authors: Ryabov Vyacheslav , Samoilova Yulia , Gombozhapova Aleksandra , Nesova Anastasiia , Kologrivova Irina
Published: November 2024

Background. Phenotyping inflammation in ST-elevation myocardial infarction (STEMI) is a challenge for modern cardiology. NLRP3 inflammasome is a proven predictor of adverse outcomes in cardiovascular disease, but its specificity in stratifying inflammatory activity in patients with myocardial infarction (MI) has not been demonstrated. The aim of this paper is to describe the levels of NLRP3 protein and IL-1β concentrations and their changes in dynamics and associations with clinical, laboratory and instrumental characteristics of patients with STEMI. Methods. A total of 45 patients with STEMI were enrolled. Concentrations of NLRP3 and IL-1β were evaluated in arterial and venous EDTA blood from the infarct-related coronary and peripheral arteries and veins on days 1, 3 and 7 after MI. Results and Conclusions. The concentrations of markers were higher on the first day after MI with a maximum decrease on the third day. The levels of both markers in venous plasma correlated with those in arterial blood, allowing their routine determination in venous plasma on the first day after MI. IL-1β levels correlated directly with the wall motion index and inversely with left ventricular ejection fraction and stroke volume, which characterize the potential contribution to adverse myocardial remodeling. There were two multidirectional trends in changes in NLRP3 and IL-1β levels during hospitalization. Initially higher levels with a gradual decrease by day 7 were associated with a longer duration of myocardial ischemia and higher plasma troponin I levels. Further evaluation of the long-term outcomes of MI will allow identifying inflammatory factors that input to the development of secondary major adverse cardiac events and will provide a new step in the understanding of inflammatory phenotyping.
Considering Biomarkers of Neurodegeneration in Alzheimer’s Disease: The Potential of Circulating Cell-Free DNA in Precision Neurology
Authors: Pollard Chad A. , Saito Erin R. , Burns Jeffrey M. , Hill Jonathon T. , Jenkins Timothy G.
Published: November 2024

Neurodegenerative diseases, such as Alzheimer’s disease (AD), are a growing public health crisis, exacerbated by an aging global population and the lack of effective early disease-modifying therapies. Early detection of neurodegenerative disorders is critical to delaying symptom onset and mitigating disease progression, but current diagnostic tools often rely on detecting pathology once clinical symptoms have emerged and significant neuronal damage has already occurred. While disease-specific biomarkers, such as amyloid-beta and tau in AD, offer precise insights, they are too limited in scope for broader neurodegeneration screening for these conditions. Conversely, general biomarkers like neurofilament light chain (NfL) provide valuable staging information but lack targeted insights. Circulating cell-free DNA (cfDNA), released during cell death, is emerging as a promising biomarker for early detection. Derived from dying cells, cfDNA can capture both general neurodegenerative signals and disease-specific insights, offering multi-layered genomic and epigenomic information. Though its clinical potential remains under investigation, advances in cfDNA detection sensitivity, standardized protocols, and reference ranges could establish cfDNA as a valuable tool for early screening. cfDNA methylation signatures, in particular, show great promise for identifying tissue-of-origin and disease-specific changes, offering a minimally invasive biomarker that could transform precision neurology. However, further research is required to address technological challenges and validate cfDNA’s utility in clinical settings. Here, we review recent work assessing cfDNA as a potential early biomarker in AD. With continued advances, cfDNA could play a pivotal role in shifting care from reactive to proactive, improving diagnostic timelines and patient outcomes.
Integrating Remote Sensing and GIS-Based Map Analysis in Determining Spread of Built-Ups and Land-Use Dynamics of Terrain of Onitsha Metropolis, Anambra State, Nigeria
Authors: Ayadiuno Romanus Udegbunam , Ndulue Dominic Chukwuka
Published: November 2024

Land scarcity in most cases hampers development and encourages the misuse of land. The suitability of land must be considered before appropriating or allocating land for any use. Land supports the livelihood of every being on the Earth and therefore determines survival, success, and sustainability (sustainable living). This study aimed at integrating remote sensing and GIS-based analysis to determine the rate at which built-up areas have spread across the terrain of Onitsha Metropolis, Anambra State, Nigeria, and the dynamics of other land uses. This research involved both primary and secondary data. The primary data included measurements, direct field observations, and key informant interviews to understand people’s perceptions of the land use in the area. The secondary data included satellite images of the area obtained from USGS and analyzed using ArcGIS 10.2 for variations in the terrain of the Onitsha Metropolis; to determine the land use and land cover change (LULCC) of the Onitsha Metropolis over 40 years, published and unpublished articles and books were also consulted. The geological analysis of the study showed that the area of the Ogwashi/Asaba formation is 318.57 km2; the areas of the Nanka sands and Bende-Ameke are 423.07 km2 and 259.42 km2, respectively. The Nanka sands and Bende-Ameke formations are best suited for engineering construction purposes, while the Ogwashi/Asaba formation is suitable for agriculture and should be designated as a buffer zone or park. However, due to the unavailability of land as a result of the growing population and the proximity of the area to the city center, the area is being encroached upon, and a large area (about 30.40%) has been converted to built-up areas as of 2022. Forecast analysis showed that if the trend continues, 158.28 km2 (49.68%) of the alluvium soils of the Ogwashi/Asaba formation will be covered with buildings by 2072. The geology and the terrain of the Onitsha Metropolis determine the soil characteristics and the land use suitability; mapping the geological formations and overlaying these with the land use and land cover change of the area revealed the extent of the encroachment on the Ogwashi/Asaba formation, which must be discouraged.
Part-Time Farming, Agricultural Socialized Services, and Organic Fertilizer Use: Implications for Climate Change Mitigation
Authors: Huang Qi , Mazhar Saman , Chen Jingjing , Mustafa Ghulam , Lin Guanghua
Published: November 2024

The adoption of organic fertilizers is essential for advancing China’s green agricultural transformation, ensuring food security, and supporting agricultural adaptations. However, several challenges hinder its widespread use in rural areas. This study examines how part-time farming and agricultural service provision influences organic fertilizer use, employing fixed and random effects models on data from 523 households in Shaanxi Province, one of China’s main apple-producing regions. The results reveal: (1) Part-time farming reduces organic fertilizer use by 7.6%, primarily due to labor shortages; (2) Higher non-farm income exacerbates this decline, particularly for Type II part-time farmers; and (3) Mechanized fertilization services help mitigate this reduction. These findings offer valuable policy insights for promoting organic fertilizer adoption in the context of shifting rural labor dynamics and highlight the complex interactions between farming practices and labor migration in the broader trajectory of organic fertilizer use. Moreover, this study highlights the role of organic fertilizer use in enhancing food security while also helping to reduce the carbon footprint of the crop sector in China.
The Impacts of Beaver Dams on Groundwater Regime and Habitat 6510
Authors: Oleszczuk Ryszard , Bajkowski Sławomir , Urbański Janusz , Pawluśkiewicz Bogumiła , Małuszyński Marcin J. , Małuszyńska Ilona , Jadczyszyn Jan , Hewelke Edyta
Published: November 2024

Changes in land usage, increasing climatic uncertainty, and dynamic development of the rate of natural population growth of the Eurasian beaver will lead to increasing benefits and disadvantages from beaver activity. During three growing seasons from 2020 to 2022, four cross-sections were marked on unused sub-irrigation systems with the periodic occurrence of beaver dams, located on organic soils in parts of the facility protected by the Habitats Directive (natural habitat 6510) in Central Poland. Periodic water table measurements in wells, the beds of adjacent ditches, and the riverbed were carried out. Identification of the states and structures of plant communities was done using the botanical-weight analysis of several samples with an area of 1 m2. Beaver dams increased water levels in the river, ditches, and groundwater depth in over 78% of events in 2020–2022 years. A large impact of precipitation on the hydraulic conditions in the meadow was observed. In the studied area, since a moderately moist habitat (6510) is protected within the Natura 2000 network, phenomena increasing soil moisture, in the absence of mowing of meadows and the occurrence of expansive herbaceous vegetation that tolerates increased moisture, may lead to the disappearance of these habitats, especially in the zone near the riverbed.
Detection of Ochratoxin A in Maize and Its Potential Impact on Avian Pathology in Romanian Farms
Authors: Beia Silviu-Ionut , Ion Violeta Alexandra , Gagniuc Elvira , Bujor Oana-Crina , Ivan Elena Ştefania , Barbu Andreea , Pitoiu Elena , Beia Violeta Elena , Bădulescu Liliana
Published: November 2024

Ochratoxin A (OTA) is a nephrotoxic mycotoxin that commonly contaminates maize, posing significant health risks to both poultry and humans. In this study, a rapid and sensitive method utilizing ultra-performance liquid chromatography coupled with fluorescence detection (UPLC-FLD) was developed for the quantification of OTA levels in maize. The method utilizes immunoaffinity column purification for improved specificity. Accuracy and precision were validated in line with European Union Reference Laboratory (EURL-MP) guidelines, meeting regulatory standards for linearity, trueness, detection and quantification limits, precision, and uncertainty, as per European Commission Regulation (EC) No. 401/2006 and its amendments. The method demonstrated an average recovery rate of 116.78% for maize, with RSDwR values (within-laboratory reproducibility) of 12.72%. Furthermore, OTA occurrence and its possible effects were investigated in several farms in South Romania, where necropsy and histopathological analyses of poultry revealed severe kidney damage, including renal tubular degeneration.