bluuebunny/crossref_metadata_2025 huggingface dataset:
3K3A-Activated Protein C Protects the Blood-Brain Barrier and Neurons From Accelerated Ischemic Injury Caused by Pericyte Deficiency in Mice
Authors: Wang Yaoming , Kisler Kassandra , Nikolakopoulou Angeliki Maria , Fernandez Jose A. , Griffin John H. , Zlokovic Berislav V.
Published: March 2022

Pericytes, mural cells of brain capillaries, maintain the blood-brain barrier (BBB), regulate cerebral blood flow (CBF), and protect neurons against ischemic damage. To further investigate the role of pericytes in ischemia, we induced stroke by 45-min transient middle cerebral artery occlusion (tMCAo) in 6-month-old pericyte-deficient Pdgfrb + ⁣/− mice and control Pdgfrb+/+ littermates. Compared to controls, Pdgfrb + ⁣/− mice showed a 26% greater loss of CBF during early reperfusion, and 40–50% increase in the infarct and edema volumes and motor neurological score 24 h after tMCAo. These changes were accompanied by 50% increase in both immunoglobulin G and fibrinogen pericapillary deposits in the ischemic cortex 8 h after tMCAo indicating an accelerated BBB breakdown, and 35 and 55% greater losses of pericyte coverage and number of degenerating neurons 24 h after tMCAo, respectively. Treatment of Pdgfrb + ⁣/− mice with 3K3A-activated protein C (APC), a cell-signaling analog of plasma protease APC, administered intravenously 10 min and 4 h after tMCAo normalized CBF during the early reperfusion phase and reduced infarct and edema volume and motor neurological score by 55–60%, with similar reductions in BBB breakdown and number of degenerating neurons. Our data suggest that pericyte deficiency results in greater brain injury, BBB breakdown, and neuronal degeneration in stroked mice and that 3K3A-APC protects the brain from accelerated injury caused by pericyte deficiency. These findings may have implications for treatment of ischemic brain injury in neurological conditions associated with pericyte loss such as those seen during normal aging and in neurodegenerative disorders such as Alzheimer’s disease.
The Role of Leaky Gut in Functional Dyspepsia
Authors: Wauters Lucas , Ceulemans Matthias , Schol Jolien , Farré Ricard , Tack Jan , Vanuytsel Tim
Published: March 2022

Patients with functional dyspepsia (FD) complain of epigastric symptoms with no identifiable cause. Increased intestinal permeability has been described in these patients, especially in the proximal small bowel or duodenum, and was associated with mucosal immune activation and symptoms. In this review, we discuss duodenal barrier function, including techniques currently applied in FD research. We summarize the available data on duodenal permeability in FD and factors associated to increased permeability, including mucosal eosinophils, mast cells, luminal and systemic factors. While the increased influx of antigens into the duodenal mucosa could result in local immune activation, clinical evidence for a causal role of permeability is lacking in the absence of specific barrier-protective treatments. As both existing and novel treatments, including proton pump inhibitors (PPI) and pre- or probiotics may impact duodenal barrier function, it is important to recognize and study these alterations to improve the knowledge and management of FD.
Association of Dietary and Lifestyle Inflammation Score With Cardiorespiratory Fitness
Authors: Farazi Mena , Jayedi Ahmad , Noruzi Zahra , Janbozorgi Nasim , Djafarian Kurosh , Shab-Bidar Sakineh
Published: March 2022

Objective

We aimed to assess the potential association of dietary (DIS) and lifestyle inflammation score (LIS) and their joint association (DLIS) with cardiorespiratory fitness (CRF) in Tehranian adults.

Design

The present study was designed cross-sectional.

Participants

A total of 265 males and females aged 18–70 years (mean ± SD: 36.9 ± 13.3) were entered in the present cross-sectional study. Eligible participants were healthy men and women who were free of medications and had no acute or chronic infection or inflammatory disease.

Measures

The DIS was calculated by the use of data from 18 anti- and pro-inflammatory dietary components, and the LIS by three non-dietary components including physical activity, smoking status, and general adiposity, with higher scores indicating a more pro-inflammatory diet and lifestyle, respectively. The DLIS was calculated by summing the DIS and LIS. CRF was assessed by the Bruce protocol and VO2 max was measuredas the main variable of CRF. The odds ratio (OR) and 95% confidence interval (CI) of CRF across tertiles of the DIS, LIS, and DLIS were estimated by logistic regression analysis with considering age, gender, energy intake, marital and education status, and occupation as confounders.

Results

The DLIS ranged from −2.10 to 0.38 (mean ± SD: −1.25 ± 0.64). In the model that controlled for all variables, the ORs of CRF for the second and third tertiles of the DLIS as compared to the first tertile were 0.42 (95%CI: 0.20, 0.90) and 0.12 (95%CI: 0.05, 0.32), respectively (P-trend < 0.001). There was a strong inverse association between the LIS and CRF (ORthirdvs.firsttertile: 0.12, 95%CI: 0.05, 0.32). There was no association between DIS and CRF.

Conclusion

The present study examined the joint association of inflammation-related lifestyle behaviors with CRF and found a strong inverse association between a pro-inflammatory lifestyle with CRF. We did not find any association between dietary inflammatory properties with CRF. Future studies should address the relationship between the inflammatory potential of the diet and CRF.
Effects of High-Fructose Corn Syrup on Bone Health and Gastrointestinal Microbiota in Growing Male Mice
Authors: Han Xiaoqiang , Feng Zhiguo , Chen Yizhang , Zhu Liying , Li Xiaoqiong , Wang Xin , Sun Haibiao , Li Jinjun
Published: March 2022

Here, we explored the correlation between gut microbiota and bone health and the effects of high-fructose corn syrup (HFCS) on both. Sixteen 3-week-old male C57BL/6J mice were randomly divided into two groups and given purified water (control group) or 30% HFCS in water (HFCS group) for 16 weeks. The effects of HFCS were assessed via enzyme-linked immunosorbent assays, histopathological assays of colon and bone, and 16S rDNA sequence analysis of gut microbiota. The serum of HFCS group mice had lower levels of bone alkaline phosphatase (BALP), bone Gla protein (BGP), insulin-like growth factor 1 (IGF-1), and testosterone, and higher levels of type I collagen carboxyl-terminal telopeptide (ICTP) and tartrate-resistant acid phosphatase (TRAP) than that of the control group. HFCS caused trabecular bone damage by decreasing trabecular number and thickness and increasing trabecular separation. The HFCS group colons were shorter than the control group colons. The HFCS-fed mice showed mild, localized shedding of epithelial cells in the mucosal layer, focal lymphocytic infiltration of the lamina propria, mild submucosal edema, and loosely arranged connective tissue. The HFCS group displayed lower abundance and altered composition of gut microbiota. The abundance of Defluviitaleaceae UCG-011, Erysipelatoclostridium, Ruminococcaceae UCG-009, Lactobacillus, Blautia, and Parasutterella increased, positively correlating with BALP, BGP, IGF-1, and testosterone levels, and negatively correlating with ICTP and TRAP levels. Our study revealed a potential diet-gut microbiota-bone health axis.
Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains
Authors: Wang Xiaoyu , Liu Guoqing , Xie Shouqi , Pan Lei , Tan Qingsong
Published: March 2022

The aim of this study was to investigate the effect of dietary protein level (soybean meal) on growth performance, flesh quality of grass carp, and the related molecular mechanisms. The results showed that appropriate dietary protein levels improved the growth performance, hardness, and pH of muscle while decreasing muscle crude lipid content and cooking loss and altering the antioxidant capacity and metabolic enzymes activities. In addition, appropriate dietary protein promoted the gene expression of myhc-1, myhc-4, myf5, myod, myog, and fgf6a, whereas inhibited that of myhc-7, myhc-2, mrf4, and mstn. Transcriptome profiling of muscle revealed that the flesh quality-specific differences were related to tight junctions and intramuscular fat (IMF) accumulation. GSEA showed that fatty acid metabolism and oxidative phosphorylation were downregulated in SM5 compared with SM1. To conclude, appropriate protein levels improved the growth and flesh quality by regulating muscle antioxidant capacity and gene expression of myhcs and fat metabolism-related signaling molecules.
Preoperative Folate Receptor-Positive Circulating Tumor Cells Are Associated With Occult Peritoneal Metastasis and Early Recurrence in Gastric Cancer Patients: A Prospective Cohort Study
Authors: Zeng Ci Dian Dan , Jin Cheng Cheng , Gao Chun , Xiao Ai Tang , Tong Yi Xin , Zhang Sheng
Published: March 2022

Background

The aim of this study is to explore the clinical feasibility of detecting folate receptor-positive circulating tumor cells (FR+ CTCs) for predicting peritoneal metastasis and short-term outcome in gastric cancer patients.

Methods

This is a prospective, single-center, observational study. We applied ligand-targeted enzyme-linked polymerization method to detect preoperative FR+ CTC levels in peripheral blood. We evaluated the diagnostic value of FR+ CTCs and other biomarkers in predicting peritoneal metastasis. Prognostic factors for recurrence-free survival (RFS) were investigated in univariate and multivariate analyses.

Results

A total of 132 patients with gastric cancer and 9 patients with benign disease were recruited. Gastric cancer patients had a significantly higher CTC level compared to that of patients with benign disease (p < 0.01). Combined model including CTC level and other biomarkers presented high sensitivity (100%) and moderate specificity (59.3%) in predicting peritoneal metastasis. Univariate analysis revealed that decreased serum prealbumin, decreased peripheral lymphocyte count, FR+ CTCs, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and lymph node metastasis were significantly associated with shorter RFS. FR+ CTC level [≥12.6 folate units (FU)/3 ml, hazard ratio (HR) = 6.957, p = 0.005] and CA19-9 (>34 ng/ml, HR = 3.855, p = 0.037) were independent prognostic factors in multivariate analysis.

Conclusions

Our findings for the first time suggested the diagnostic value of preoperative CTC levels in predicting peritoneal metastasis in gastric cancer. Moreover, the FR+ CTC level could be a novel and promising prognostic factor for the recurrence of gastric cancer in patients who underwent surgery.

Clinical Trial Registration

Chinese Clinic Trial Registry, identifier ChiCTR2100050514.
Identification of LncRNA Prognostic Signature Associated With Genomic Instability in Pancreatic Adenocarcinoma
Authors: Zhu Jinfeng , Huang Qian , Peng Xingyu , Luo Chen , Liu Sicheng , Liu Zitao , Wu Xun , Luo Hongliang
Published: March 2022

Background

Genomic instability (GI) is a critical feature of cancer which plays a key role in the occurrence and development of pancreatic adenocarcinoma (PAAD). Long non-coding RNA (LncRNA) is an emerging prognostic biomarker because it is involved in regulating GI. Recently, researchers used such GI-related LncRNAs (GILncRNAs) to establish a prognostic signature for patients with cancer and helped in predicting the overall prognosis of the patients. However, it is evident that patients with PAAD still lack such prognostic signature constructed with GILncRNA.

Methods

The present study screened GILncRNAs from 83 patients with PAAD. Prognosis-related GILncRNAs were identified by univariate Cox regression analysis. The correlation coefficients of these GILncRNAs were obtained by multivariate Cox regression analysis and used to construct a signature. The signature in the present study was then assessed through survival analysis, mutation correlation analysis, independent prognostic analysis, and clinical stratification analysis in the training set and validated in the testing as well as all TCGA set. The current study performed external clinical relevance validation of the signature and validated the effect of AC108134.2 in GILncSig on PAAD using in vitro experiments. Finally, the function of GILncRNA signature (GILncSig) dependent on Gene Ontology enrichment analysis was explored and chemotherapeutic drug sensitivity analysis was also performed.

Results

Results of the present study found that a total of 409 GILncRNAs were identified, 5 of which constituted the prognostic risk signature in this study, namely, AC095057.3, AC108134.2, AC124798.1, AL606834.1, and AC104695.4. It was found that the signature of the present study was better than others in predicting the overall survival and applied to patients with PAAD of all ages, genders, and tumor grades. Further, it was noted that the signature of the current study in the GSE102238, was correlated with tumor length, and tumor stage of patients with PAAD. In vitro, functional experiments were used in the present study to validate that AC108134.2 is associated with PAAD genomic instability and progression. Notably, results of the pRRophetic analysis in the current study showed that the high-risk group possessed reverse characteristics and was sensitive to chemotherapy.

Conclusions

In conclusion, it was evident that the GILncSig used in the present study has good prognostic performance. Therefore, the signature may become a potential sensitive biological indicator of PAAD chemotherapy, which may help in clinical decision-making and management of patients with cancer.
Quantitative Dynamic-Enhanced MRI and Intravoxel Incoherent Motion Diffusion−Weighted Imaging for Prediction of the Pathological Response to Neoadjuvant Chemotherapy and the Prognosis in Locally Advanced Gastric Cancer
Authors: Zhu Yongjian , Jiang Zhichao , Wang Bingzhi , Li Ying , Jiang Jun , Zhong Yuxin , Wang Sicong , Jiang Liming
Published: March 2022

Background

This study aimed to explore the predictive value of quantitative dynamic contrast-enhanced MRI (DCE-MRI) and intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) quantitative parameters for the response to neoadjuvant chemotherapy (NCT) in locally advanced gastric cancer (LAGC) patients, and the relationship between the prediction results and patients’ prognosis, so as to provide a basis for clinical individualized precision treatment.

Methods

One hundred twenty-nine newly diagnosed LAGC patients who underwent IVIM-DWI and DCE-MRI pretreatment were enrolled in this study. Pathological tumor regression grade (TRG) served as the reference standard of NCT response evaluation. The differences in DCE-MRI and IVIM-DWI parameters between pathological responders (pR) and pathological non-responders (pNR) groups were analyzed. Univariate and multivariate logistic regressions were used to identify independent predictive parameters for NCT response. Prediction models were built with statistically significant quantitative parameters and their combinations. The performance of these quantitative parameters and models was evaluated using receiver operating characteristic (ROC) analysis. Clinicopathological variables, DCE-MRI and IVIM-DWI derived parameters, as well as the prediction model were analyzed in relation to 2-year recurrence-free survival (RFS) by using Cox proportional hazards model. RFS was compared using the Kaplan–Meier method and the log-rank test.

Results

Sixty-nine patients were classified as pR and 60 were pNR. Ktrans, kep, and ve values in the pR group were significantly higher, while ADCstandard and D values were significantly lower than those in the pNR group. Multivariate logistic regression analysis demonstrated that Ktrans, kep, ve, and D values were independent predictors for NCT response. The combined predictive model, which consisted of DCE-MRI and IVIM-DWI, showed the best prediction performance with an area under the curve (AUC) of 0.922. Multivariate Cox regression analysis showed that ypStage III and NCT response predicted by the IVIM-DWI model were independent predictors of poor RFS. The IVIM-DWI model could significantly stratify median RFS (52 vs. 15 months) and 2-year RFS rate (72.3% vs. 21.8%) of LAGC.

Conclusion

Pretreatment DCE-MRI quantitative parameters Ktrans, kep, ve, and IVIM-DWI parameter D value were independent predictors of NCT response for LAGC patients. The regression model based on baseline DCE-MRI, IVIM-DWI, and their combination could help RFS stratification of LAGC patients.
Exploring Histological Similarities Across Cancers From a Deep Learning Perspective
Authors: Menon Ashish , Singh Piyush , Vinod P. K. , Jawahar C. V.
Published: March 2022

Histopathology image analysis is widely accepted as a gold standard for cancer diagnosis. The Cancer Genome Atlas (TCGA) contains large repositories of histopathology whole slide images spanning several organs and subtypes. However, not much work has gone into analyzing all the organs and subtypes and their similarities. Our work attempts to bridge this gap by training deep learning models to classify cancer vs. normal patches for 11 subtypes spanning seven organs (9,792 tissue slides) to achieve high classification performance. We used these models to investigate their performances in the test set of other organs (cross-organ inference). We found that every model had a good cross-organ inference accuracy when tested on breast, colorectal, and liver cancers. Further, high accuracy is observed between models trained on the cancer subtypes originating from the same organ (kidney and lung). We also validated these performances by showing the separability of cancer and normal samples in a high-dimensional feature space. We further hypothesized that the high cross-organ inferences are due to shared tumor morphologies among organs. We validated the hypothesis by showing the overlap in the Gradient-weighted Class Activation Mapping (GradCAM) visualizations and similarities in the distributions of nuclei features present within the high-attention regions.
Identification of a Prognostic Signature Composed of GPI, IL22RA1, CCT6A and SPOCK1 for Lung Adenocarcinoma Based on Bioinformatic Analysis of lncRNA-Mediated ceRNA Network and Sample Validation
Authors: Tang Wenjun , Lu Qiaonan , Zhu Jianling , Zheng Xiaowei , Fang Na , Ji Shaoping , Lu Feng
Published: March 2022

Lung adenocarcinoma (LUAD) is one of the most common malignant tumors with high morbidity and mortality in China and worldwide. Long non-coding RNAs (lncRNAs) as the competing endogenous RNA (ceRNA) play an essential role in the occurrence and development of LUAD. However, identifying lncRNA-related biomarkers to improve the accuracy of LUAD prognosis remains to be determined. This study downloaded RNA sequence data from The Cancer Genome Atlas (TCGA) database and identified the differential RNAs by bioinformatics. A total of 214 lncRNA, 198 miRNA and 2989 mRNA were differentially identified between LUAD and adjacent nontumor samples. According to the ceRNA hypothesis, we constructed a lncRNA-miRNA-mRNA network including 95 protein-coding mRNAs, 7 lncRNAs and 15 miRNAs, and found 24 node genes in this network were significantly associated with the overall survival of LUAD patients. Subsequently, through LASSO regression and multivariate Cox regression analyses, a four-gene prognostic signature composed of GPI, IL22RA1, CCT6A and SPOCK1 was developed based on the node genes of the lncRNA-mediated ceRNA network, demonstrating high performance in predicting the survival and chemotherapeutic responses of low- and high-risk LUAD patients. Finally, independent prognostic factors were further analyzed and combined into a well-executed nomogram that showed strong potential for clinical applications. In summary, the data from the current study suggested that the four-gene signature obtained from analysis of lncRNA-mediated ceRNA could serve as a reliable biomarker for LUAD prognosis and evaluation of chemotherapeutic response.
Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity
Authors: Araujo Nathalia , Sledziona James , Noothi Sunil K. , Burikhanov Ravshan , Hebbar Nikhil , Ganguly Saptadwipa , Shrestha-Bhattarai Tripti , Zhu Beibei , Katz Wendy S. , Zhang Yi , Taylor Barry S. , Liu Jinze , Chen Li , Weiss Heidi L. , He Daheng , Wang Chi , Morris Andrew J. , Cassis Lisa A. , Nikolova-Karakashian Mariana , Nagareddy Prabhakar R. , Melander Olle , Evers B. Mark , Kern Philip A. , Rangnekar Vivek M.
Published: March 2022

Prostate apoptosis response-4 (Par-4) is a tumor suppressor that induces apoptosis in cancer cells. However, the physiological function of Par-4 remains unknown. Here we show that conventional Par-4 knockout (Par-4-/-) mice and adipocyte-specific Par-4 knockout (AKO) mice, but not hepatocyte-specific Par-4 knockout mice, are obese with standard chow diet. Par-4-/- and AKO mice exhibit increased absorption and storage of fat in adipocytes. Mechanistically, Par-4 loss is associated with mdm2 downregulation and activation of p53. We identified complement factor c3 as a p53-regulated gene linked to fat storage in adipocytes. Par-4 re-expression in adipocytes or c3 deletion reversed the obese mouse phenotype. Moreover, obese human subjects showed lower expression of Par-4 relative to lean subjects, and in longitudinal studies, low baseline Par-4 levels denoted an increased risk of developing obesity later in life. These findings indicate that Par-4 suppresses p53 and its target c3 to regulate obesity.
Chylous Leakage After Breast-Conserving Surgery and Axillary Clearance: Case Report and Management Strategies
Authors: Yin Liang , Chen Peiqing , Agyekum Enock Adjei , Xiao Xiudi , Qian Xiaoqin
Published: March 2022

Chylous leakage is a rare complication of breast and axillary surgery. We present a case of chylous leakage inside the breast following breast-conserving surgery and axillary lymph node dissection. The majority of chylous leakages in the breast are managed with conservative measures aimed at reducing lymphatic fluid production and outflow. Surgical intervention is required in cases of conservative treatment failure and high output chylous leakage. To the best of our knowledge, this is the first case report of chyles leaks inside the breast following breast-conserving surgery that was successfully treated surgically.
Establishment of Cutoff Values for Newborn Screening of Six Lysosomal Storage Disorders by Tandem Mass Spectrometry
Authors: Li Ruotong , Tian Liping , Gao Qing , Guo Yuanfang , Li Gaijie , Li Yulin , Sun Meng , Yan Yan , Li Qing , Nie Wenying , Zou Hui
Published: March 2022

Objective

Lysosomal storage disorders (LSDs) are becoming increasingly important in newborn screening, and tandem mass spectrometry (MS/MS) is widely used in newborn screening for LSDs through measurement of enzymatic activities in dried blood spots (DBSs). Overall, the determination of the cutoff value is important in such screening, and different laboratories have different methods of determining this value; most do not use a fixed cutoff value but rather calculate the corresponding batch cutoff value based on each batch of experimental data. In this study, we used MS/MS to screen for LSDs and sought to find an appropriate method to establish the cutoff value for LSD screening.

Methods

A total of 38,945 samples from newborn blood tablets collected from various maternity hospitals in six cities in Shandong province, including Jinan, Dezhou, Heze, Linyi, Weifang, and Zibo, were tested using a Waters Xevo TQD tandem mass spectrometer; the experimental data were analyzed with MassLynx V4.1. The laboratory used 30% of the median GLA enzyme activity and 20% of the median ABG, ASM, GALC, IDUA, and GAA enzyme activities in every test as the cutoff values for that batch of experiments.

Results

There were 254 suspicious positives in the initial screening test, including one case of Gaucher disease, one of Niemann-Pick disease, 47 of Krabbe disease, four of MPS-I, 21 of Fabry disease, and 180 of Pompe disease. After genetic screening, 11 children were diagnosed, including three with Pompe disease, three with Fabry disease, and five with Krabbe disease. In addition, the enzyme activity cutoff value of this experiment showed seasonal variation, which was initially believed to be related to the ambient temperature, such as the effect of ambient temperature on the human body or the temperature when the blood tablets dried naturally.

Conclusion

Overall, MS/MS can be used in LSD screening, and using different cutoff values in each batch of experiments is feasible. The ambient temperature might be a reason why the enzyme activity cutoff value has seasonal variation. More samples are needed to develop a method of determining cutoff values in laboratories.
Instruments Measuring Self-Care in Children and Young Adults With Chronic Conditions: A Systematic Review
Authors: Biagioli Valentina , Spitaletta Giuseppina , Kania Valeria , Mascolo Rachele , Gawronski Orsola , Liburdi Annachiara , Manzi Giulia , Salata Michele , Vellone Ercole , Tiozzo Emanuela , Dall’Oglio Immacolata
Published: March 2022

Children and young adults (CYAs) with chronic conditions need to engage in self-care to improve their quality of life. This study aimed to retrieve the literature on instruments to assess self-care in CYAs living with chronic conditions and evaluate the psychometric proprieties of the instruments retrieved. A systematic literature review was conducted on six databases to identify peer-reviewed papers that described or used an evaluation instrument of self-care in CYAs with chronic conditions. Twenty-three articles describing 11 instruments of self-care were identified. Five instruments (45.45%) were developed for specific diseases, while six (54.54%) for various chronic illnesses. Most of the instruments were focused on treatment adherence within self-care maintenance (i.e., behaviors to maintain illness stability), excluding the monitoring of clinical parameters or the management of exacerbations. This review provides an overview of available instruments that measure self-care in CYAs with chronic conditions, which health professionals could use for patient education.
Janus Kinase Mediates Faster Recovery From Sevoflurane Anesthesia Than Isoflurane Anesthesia in the Migratory Locusts
Authors: Ma Zongyuan , Zheng Jialin C. , Li Tianzuo , Xie Zhongcong , Kang Le
Published: March 2022

Inhalation anesthetics isoflurane and sevoflurane have been widely used in clinical practice for anesthesia. However, the molecular mechanisms underlying the faster recovery from sevoflurane anesthesia than isoflurane anesthesia remain largely undetermined. Herein, we use RNA-seq, RNA interference, quantitative real-time PCR and western blotting to explore the mechanisms of recovery from isoflurane and sevoflurane anesthesia in the migratory locusts. Although the migratory locusts show similar anesthetic responses to these two chemicals in corresponding half-maximal effective concentrations (EC50s), the recovery from sevoflurane anesthesia is significantly faster than that for isoflurane anesthesia after 30 min of anesthetic exposure. Transcriptome analysis shows that those transcripts involved in cytoskeletal components, Janus kinase (JAK) pathway and cuticle protein are differentially expressed in locust brains in response to isoflurane and sevoflurane. RNAi knockdown confirms thatActin,Myosin-like protein 84B(Mlp84B),JAKand cuticle proteinNCP56do not affect anesthetic response of the locusts to these two chemical anesthetics. Moreover,actin,Mlp84BandNCP56do not affect differential recovery from isoflurane and sevoflurane anesthesia, whereas RNAi knockdown ofJAKand its partnerSTAT5Bdoes not affect anesthetic recovery from isoflurane but elongates recovery duration from sevoflurane anesthesia. Thus, JAK may mediate faster recovery from sevoflurane anesthesia than from isoflurane anesthesia in the migratory locust. This finding provides novel insights into the molecular mechanism underlying faster recovery from sevoflurane anesthesia than isoflurane anesthesia.
The Joint Effects of Social Norm Appeals and Fear Appeals in COVID-19 Vaccine Campaign Posters on Self-Perceived Communication Quality and Vaccination Intention
Authors: Liu Jiawei , Yang Xiaobing , Lu Yanqin , Zheng Xia
Published: March 2022

To understand how different types of cues in vaccine education messages affect attitude toward campaign messages and vaccination intention, this study examined the impact of the presence of social norm appeals (individual vs. group cues) and the presence of fear appeals in coronavirus disease 2019 (COVID-19) vaccine campaign posters on perceived communication quality and vaccination intention. A 2 (social norm appeal: individual cue vs. group cue) × 2 (fear appeal: absence vs. presence) × 3 (repetition) within-subject factorial design experiment was conducted in China. Findings demonstrated that the presence of fear appeals in COVID-19 vaccine campaign posters elicited lower levels of perceived communication quality and vaccination intention than those without fear appeals. The interactive effect of fear appeals and social norm appeals was also found to be significant. Specifically, positive-framed messages (i.e., absence of fear appeals) with group cues and fear appeal messages with individual cues elicited higher perceived information quality and stronger vaccination intention than other types of messages. Understanding how these cues function jointly in COVID-19 vaccine campaign messages will help public health practitioners create more effective intervention strategies.
Design of a Participatory Organizational-Level Work Stress Prevention Approach in Primary Education
Authors: Bakhuys Roozeboom Maartje C. , Niks Irene M. W. , Schelvis Roosmarijn M. C. , Wiezer Noortje M. , Boot Cécile R. L.
Published: March 2022

Background

Work stress is a serious problem in primary education. Decades of research underline the importance of participatory, organizational-level work stress prevention approaches. In this approach, measures are planned to tackle causes of work stress in a participatory manner and implemented by a working group consisting of members of the organization. This approach can only be effective if the measures contain effective ingredients to decrease work stress risks and are successfully implemented. The aim of this paper is to present an outline of a work stress prevention approach that is evaluated in primary education. To ensure the appropriateness of measures, a logic model of change is built as part of the risk assessment to facilitate the selection of appropriate measures. Progression on target behaviors as well as implementation factors are real-time monitored during implementation and fed back to the working groups, to provide the opportunity to adjust action plans when needed to optimize implementation.

Methods

The approach consists of five steps: (1) preparation: installing an advisory board and working groups, (2) risk assessment: inventory of work stress risks (questionnaires and focus groups). In addition, a behavioral analysis is performed to build a logic model of change to facilitate selection of measures, (3) action planning: conducting an action plan with appropriate measures (focus groups), (4) implementation: implementing the action plan. During implementation progression on target behaviors and implementation factors are monthly monitored and fed back to the working groups, and (5) evaluation: effects of the approach are studied in a controlled trial with measurements at baseline (T0), 1 year (T1), and 2 years (T2) follow-up. A process evaluation is carried out using quantitative (questionnaires and real-time monitoring data) and qualitative (interviews and data logs) data to study the implementation process of all steps of the work stress approach.

Discussion

We believe that building a logic model of change and real-time monitoring of implementation could be of added value to improve the success of the work stress prevention approach. With this study, we aim to provide more insights into work stress intervention research, especially in primary education.

Clinical Trial Registration

The study is registered in Netherlands Trial Register (ClinicalTrials.gov #NL9797, October 18, 2021).
Be in Your Element: The Joint Effect of Human Resource Management Strength and Proactive Personality on Employee Creativity
Authors: Zhang Jiexuan , Zhu Fei , Liu Ning , Cai Zijun
Published: March 2022

Employee creativity is fast becoming a part and parcel in the wake of the increasing volatility of the employment market and the complexity of job demands. Drawing from the actor-context interactionist theoretical approach and career construction theory, this paper adds to current research by exploring the serial mediating effect of job crafting (JC) and career adaptability (CA) in the impact of human resource management strength (HRMS) on employee creativity. Furthermore, we suggest that proactive personality interacts with HRMS to jointly influence creativity. Survey data from samples of 297 (Study 1) and 390 (Study 2) employees largely confirm our model. Our findings show that HRMS positively impacts employee creativityviaserial mediation of job crafting and career adaptability, and proactive personality negatively moderates the process. The paper confirms and expands the interactionist theoretical perspective of creativity, highlights the significance of integration of contextual factors, individual characteristics, and career construction, and makes certain practical sense.
The Effects of Online Mindfulness-Based Intervention on Posttraumatic Stress Disorder and Complex Posttraumatic Stress Disorder Symptoms: A Randomized Controlled Trial With 3-Month Follow-Up
Authors: Dumarkaite Austeja , Truskauskaite-Kuneviciene Inga , Andersson Gerhard , Kazlauskas Evaldas
Published: March 2022

Objectives

Mindfulness-based interventions have recently been shown to be a promising option for treating posttraumatic stress. The current study aimed to investigate the effects of an online mindfulness-based intervention on ICD-11 posttraumatic stress disorder (PTSD) and complex PTSD (CPTSD) symptoms at a 3-month follow-up.

Methods

An RCT design with three measurement points (pre-intervention, post-intervention, and 3-month follow-up) was used to investigate the effects of an 8-week online mindfulness intervention. In total, 53 traumatized young adults (Mage = 23.21, SDage = 2.81; 84.9% female) participated in the study: 17 in the intervention group and 36 in the waiting list control group.

Results

Intervention group and waiting list control group comparison revealed that the intervention was effective for reducing CPTSD disturbances in self-organization symptoms (d = −0.84 [−1.44; −0.24]), specifically, negative self-concept (d = −0.66 [−1.25; −0.07]) and disturbances in relationships (d = −0.87 [−1.47; −0.27]), at 3-month follow-up. There were no between-group effects for PTSD symptoms from pre-test to follow-up.

Conclusion

This is one of the first RCT studies to report follow-up effects of an online mindfulness-based intervention for ICD-11 PTSD or CPTSD symptoms. Our study yielded that the effects of mindfulness-based internet intervention on CPTSD symptoms tend to retain over time.

Trial Registration

This study was registered with ClinicalTrials.gov (NCT number: NCT04333667; https://clinicaltrials.gov/ct2/show/NCT04333667). Registered April 3, 2020.
Technological Applications Contributing to Relieve Care Burden or to Sleep of Caregivers and People With Dementia: A Scoping Review From the Perspective of Social Isolation
Authors: Huisman Chantal , Huisman Emelieke , Kort Helianthe
Published: March 2022

The need for care will increase in the coming years. Most people with a disability or old age receive support from an informal caregiver. Caring for a person with dementia can be difficult because of the BPSD (Behavioral and Psychological Symptoms of Dementia). BPSD, including sleep disturbance, is an important factor for a higher care load. In this scoping review, we aim to investigate whether technology is available to support the informal caregiver, to lower the care burden, improve sleep quality, and therefore influence the reduction of social isolation of informal caregivers of people with dementia. A scoping review is performed following the methodological framework by Arksey and O'Mally and Rumrill et al., the scoping review includes scientific and other sources (unpublished literature, websites, reports, etc.). The findings of the scoping review shows that there are technology applications available to support the informal caregiver of a person with dementia. The technology applications mostly contribute to lower the care burden and/or improve sleep quality and therefore may contribute to reduce social isolation. The technology applications found target either the person with dementia, the informal caregiver, or both.
Health Status and Association With Interpersonal Relationships Among Chinese Children From Urban Migrant to Rural Left-Behind
Authors: Zhao Guanlan , Wang Menmen , Xu Jiayao , Lu Jingjing , Akezhuoli Hailati , Wang Feng
Published: March 2022

Background

To date, numerous studies have examined the health status of Chinese left-behind children and migrant children. However, the impact of children's diverse migration/left-behind experiences on their health is still unclear.

Methods

A cross-sectional survey was conducted in 2020 in Nanling country (Anhui province) and Kaihua country (Zhejiang province) in China. School children from grade 5 to 8 reported their socio-demographic, interpersonal relationships, self-rated health, suicidal ideation, and depression. Participants were divided into four groups based on their migrant patterns, namely rural left-behind children with previous migration experience (ME-LBC), rural children with previous migration experience (ME-NLBC), rural left-behind children without migration experience (LBC), and rural children without migration experience (NLBC).

Results

Among 2,323 participants included in the present study, there were 336 ME-LBC (14.5%), 283 ME-NLBC (12.2%), 561 LBC (24.1%) and 1,143 NLBC (49.2%). Compared with NLBC, ME-LBC reported significantly poorer self-rated health (OR = 0.72, 95% CI [0.53–0.97], p < 0.05), higher risk of depression (β = 0.90, 95% CI [0.02–1.77], p < 0.05) with adjustment of socio-demographic and interpersonal relationships. There was no significant difference in suicidal ideation among different groups of children. The better interpersonal relationship was associated with a better self-rated health, and lower prevalence of depression and suicidal intention.

Conclusions

Compared to ordinary rural children, ME-LBC tended to experience higher levels of depression and poorer self-rated health. These research findings imply developing intervention programs about psychological adjustment tailored to different migrant patterns of Chinese rural children. The keys might be to strengthen the relationships with peer and teacher in school and improve the quality of parent-child communication in family for LBC.
Measuring Research Information Citizenship Across ORCID Practice
Authors: Porter Simon J.
Published: March 2022

Over the past 10 years, stakeholders across the scholarly communications community have invested significantly not only to increase the adoption of ORCID adoption by researchers, but also to build the broader infrastructures that are needed both to support ORCID and to benefit from it. These parallel efforts have fostered the emergence of a “research information citizenry” between researchers, publishers, funders, and institutions. This paper takes a scientometric approach to investigating how effectively ORCID roles and responsibilities within this citizenry have been adopted. Focusing specifically on researchers, publishers, and funders, ORCID behaviors are measured against the approximated research world represented by the Dimensions dataset.
Impacts of Surface Water Quality in the Awash River Basin, Ethiopia: A Systematic Review
Authors: Assegide Endaweke , Alamirew Tena , Bayabil Haimanote , Dile Yihun T. , Tessema Bezaye , Zeleke Gete
Published: March 2022

Water quality impairment, due to anthropogenic activities and limited enforcement capacity, is a rapidly growing threat to water security as well as public health in developing countries. Cumulative effects of deteriorating water quality undoubtedly put pressure on public health and socio-economic developments. For example, most industries in Ethiopia discharge their effluent directly into freshwater systems without any treatment process. The problem is severe for rivers such as the Awash that pass through major cities. Although there were a few studies that looked into the issue, there is a lack of comprehensive water quality impact assessment on agriculture, health, and socio-economics. This article systematically summarizes current research on water quality issues in the Awash River Basin to generate comprehensive information that captures the water quality status of the river and impacts of water contamination, and identify information and management gaps. Results showed that water quality degradation along the river course and in selected tributaries exceeds water quality standards by the WHO and national guidelines. For example, E-coli bacteria concentration in two tributaries, Tinishu and Tiliku Akaki, reach up to 6.68 and 6.61 billion CFU 100 ml/L. Virological profile of creeks receiving wastewater from hospitals in the City of Addis Ababa contains coliphages levels reaching as high as 5.2 × 103 pfu/100 ml for urban rivers and up to 4.92 × 103 pfu/100 ml. Heavy metals that far exceed the tolerable levels for humans were also detected in vegetables produced using impaired water. Heavy metals such as Cd, Cr, Cu, Hg, Ni, and Zn were detected in potato, Zn and Hg in Cabbage, and Cr in onion and red beet. Lettuce irrigated with Akaki river water found to contain 0.263 (Cd), 420 (Fe), 13.44 (Zn), 7.87 (Cr), 7.49 (Cu), and 6.55 (Pb) in mg/kg both in excess of WHO guideline. In addition, a high concentration of Cr has been also found in fish tissues. There has never been a systematic evaluation of the impact of contaminated water in the Awash Basin. Comprehensive impact of water quality investigation that takes into account the different pollutants dynamic needs to be made to protect the well being of downstream beneficiaries including the aquatic ecosystem. In conclusion the systematic review has shown that for a river that cross-through emerging mega-city like Addis Ababa, the human and ecosystem health impact of aquatic ecosystems pollution should not afterthought action
Move: Durational Dance-Making as We Age
Authors: Nikolai Jennifer
Published: March 2022

Dance making and moving support a positive aging approach to aesthetic, cultural, and social meanings, thus, shaping dancers' lives. My perspective is informed by making dance a durational and sustained practice in the life of a long-life mover. Positioned as a practice-oriented researcher, I am surrounded by inspiring authors and artists who embrace maturity with improvisational making. This perspective piece reflects on making Move, a durational practice-oriented research process. Move contextualizes durational elements that I associates with the life of a mover; my writing and my companions improvising with my hand-held camera in isolation, in my neighborhood on Tāmaki Makaurau/Auckland's North Shore.This perspective piece opens a process I refer to as a camera-dancer dyad, a duet between a dancer and a camera, making in isolation as we age. Furthermore, by embracing the accessibility of everyday recording devices as dancing partners, dance making with mobile cameras becomes a playful partnership with a long life.
Fijian Farmers' Attitude and Knowledge Towards Antimicrobial Use and Antimicrobial Resistance in Livestock Production Systems–A Qualitative Study
Authors: Khan Xavier , Lim Rosemary H. M. , Rymer Caroline , Ray Partha
Published: March 2022

Antimicrobial resistance (AMR) is a global health issue affecting humans and livestock. To mitigate AMR risks, responsible use of antimicrobials in livestock production systems have been advocated. Studies have reported patterns of antimicrobial use (AMU) in livestock production systems; however, there is limited information on the drivers of AMU and AMR. Therefore, this study aimed to explore and understand the attitude and knowledge of Fijian livestock farmers on AMU and AMR. Livestock farmers and managers from the Central and Western divisions of Viti Levu, Fiji were recruited using purposive and snowball sampling methods. Face-to-face one-to-one semi-structured qualitative interviews were conducted. Interview questions were informed by the Theory of Planned Behavior (TPB). Interview transcripts were analyzed inductively using reflexive thematic analysis and deductively using the TPB framework. A total of 19 cattle and poultry farmers took part. Our analysis generated four themes: (1) Uninformed use of antimicrobials and unaware of AMR, (2) Safeguarding livestock and generating income source as primary motivators for using antimicrobials (3) Medicine shortage results in hoarding and self-prescribing, and (4) Farm decisions on AMU and livestock management influenced by foreign farmers and veterinarians. Livestock farmers used medicines in livestock production; however, they could not differentiate amongst different types of medicine, including antimicrobials. Antimicrobials were used to prevent diseases in livestock and promote production of food and financial security but without any awareness of the risks of AMR. Additionally, farmers hoarded and self-prescribed medicines. Farmers rationed antimicrobials by not completing the entire course of antibiotics to save them for future use. Based on past experiences, farmers expressed dissatisfaction with the veterinary services provided by the government. They sought help online and from foreign farmers and veterinarians. We propose the need for antimicrobial stewardship (AMS) programmes focused on promoting rational use of antimicrobials and awareness of AMR amongst farmers in the Fijian livestock production systems. These programmes need to consider the anthropological, socio-cultural, economic, and environmental factors driving AMU. Future studies are underway to explore the attitude and knowledge of Fijian veterinarians, para-veterinarians and pharmacists on AMU and AMR to gain a broader systems knowledge to inform the design of AMS programmes.
An Integrated Analysis of Dairy Farming: Direct and Indirect Environmental Interactions in Challenging Bio-Physical Conditions
Authors: Triky Shir , Kissinger Meidad
Published: March 2022

The demand for milk and its products is growing worldwide. The need to find more efficient ways to produce milk while reducing pressure on the local and global environment has been identified. The Israeli dairy system operates in a challenging environment (limited land, water, and a harsh climate). This paper embraces a life cycle assessment (LCA) framework to analyze various local and global direct and indirect environmental interactions of milk production in Israel. The results show that the production of 1 kg of fat and protein-corrected milk (FPCM) in the systems that were analyzed requires on average 0.5 m2 of land, 52 L of water, and 3.3 MJ of energy. The emissions that were generated over the life cycle averaged 1.03 kg CO2-eq (GWP), 0.0095 kg SO2-eq (AP) and 0.003 kg PO4-eq (EP). The research findings point to several ‘pollution hotspots’ that are relevant also to dairy systems in other regions, including feed supply, GHG emissions that are related to enteric fermentation, manure management, and the use of water, and discuss some potential directions to advance more efficient, less polluting system.