bluuebunny/crossref_metadata_2025 huggingface dataset:
Pleiotropic Devitalization of Renal Cancer Cells by Non-Invasive Physical Plasma: Characterization of Molecular and Cellular Efficacy
Authors: Nitsch Andreas , Sander Caroline , Eggers Benedikt , Weiss Martin , Egger Eva , Kramer Franz-Josef , Erb Holger H. H. , Mustea Alexander , Stope Matthias B.
Published: January 2023

Renal cell carcinoma (RCC) is the third most common urological tumor and has an extremely poor prognosis after metastasis has occurred. Therapeutic options are highly restricted, primarily due to resistance to classical chemotherapeutics. The development of new, innovative therapeutic procedures is thus of great urgency. In the present study, the influence of non-invasive physical plasma (NIPP) on malignant and non-malignant renal cells is characterized. The biological efficacy of NIPP has been demonstrated in malignant renal cell lines (786-O, Caki-1) and non-malignant primary human renal epithelial cells (HREpC). The cell responses that were experimentally examined were cell growth (cell number determination, calculation of growth rate and doubling time), cell motility (scratch assay, invasiveness assay), membrane integrity (uptake of fluorescent dye, ATP release), and induction of apoptosis (TUNEL assay, caspase-3/7 assay, comet assay). A single NIPP treatment of the malignant cells significantly inhibited cell proliferation, invasiveness, and metastasis. This treatment has been attributed to the disruption of membrane functionality and the induction of apoptotic mechanisms. Comparison of NIPP sensitivity of malignant 786-O and Caki-1 cells with non-malignant HREpC cells showed significant differences. Our results suggest that renal cancer cells are significantly more sensitive to NIPP than non-malignant renal cells. Treatment with NIPP could represent a promising innovative option for the therapy of RCC and might supplement established treatment procedures. Of high clinical relevance would be the chemo-sensitizing properties of NIPP, which could potentially allow a combination of NIPP treatment with low-dose chemotherapy.
Orientation Behavior of Nematic Liquid Crystals at Flow-Wall Interfaces in Microfluidic Channels
Authors: Bezrukov Artem , Galyametdinov Yury
Published: January 2023

This work characterizes the orientation behavior of nematic liquid crystals in pressure-driven flows of microfluidic channels at interfaces between the flow and microchannel walls. The impact of flow velocity and microchannel geometry on the orientation of liquid crystals in single-phase and two-phase flows is discussed. Polarizing optical microscopy images revealed the homeotropic orientation of liquid crystal molecules at microchannel walls at zero flow velocities, which gradually transitioned into planar alignment along the microchannel axis when the flow velocity increased in the 50 μm/s to 5 mm/s range. Liquid crystal droplets demonstrated homeotropic or planar alignment depending on the sizes of droplets and flow velocities. The polarized light pattern from homeotropically aligned droplets deposited on microchannel walls was found to be logarithmically proportional to the flow velocity in the 2 to 40 mm/s range. The revealed behavior of nematic liquid crystals at microchannel wall surfaces in dynamic flow conditions offers new tools for on-demand control of the optical properties of microfluidic devices and can contribute to the development of analytical lab-on-chip tools with internal continuous or discrete liquid crystal layers for flow characterization in microchannel confinement.
“Urethral-Sparing” Robotic Radical Prostatectomy: Critical Appraisal of the Safety of the Technique Based on the Histologic Characteristics of the Prostatic Urethra
Authors: Asimakopoulos Anastasios D. , Annino Filippo , Colalillo Gaia , Gaston Richard , Piechaud Thierry , Mauriello Alessandro , Anceschi Umberto , Borri Filippo
Published: January 2023

Background: The prostatic urethra (PU) is conventionally resected during robot-assisted radical prostatectomy (RALP). Recent studies demonstrated the feasibility of the extended PU preservation (EPUP). Aims: To describe the histologic features of the PU. Methods: The PU was evaluated using cystoprostatectomy and RALP specimens. Cases of PU infiltration by prostate cancer or distortion by benign hyperplastic nodules were excluded. The thickness of the chorion and distance between the urothelium and prostate glands were measured. Prostate-specific antigen expression in the PU epithelium was evaluated with immunohistochemistry. Descriptive statistics were used. Results: Six specimens of PU were examined. Histologically, the following layers of the PU were observed: (1) urothelium with basal membrane, (2) chorion, and (3) prostatic peri-urethral fibromuscular tissue. The chorion measures between 0.2 and 0.4 mm. There is not a distinct urethral muscle layer, but rather muscular fibers that originate near the prostatic stroma and are distributed around the PU. This muscular tissue appears to be mainly represented in the basal and apical urethra, but not in the middle urethra. The mean distance between the chorion and prostatic glands is 1.74 mm, with significant differences between base of the prostate, middle urethral portion, and apex (2.5 vs. 1.49 vs. 1.23 mm, respectively). PSA-expressing cells are abundant in the PU epithelium, coexisting with urothelial cells. Conclusions: The exiguity of thickness of the PU chorion, short distance from glandular tissue, and coexistence of PSA-expressing cells in the epithelium raise important concerns about the oncologic safety of EPUP.
Two New Phyllopodopsyllus Species (Harpacticoida, Tetragonicipitidae) from Korea
Authors: Kim Jaehyun , Moon Heejin , Bang Hyun Woo , Lee Wonchoel
Published: January 2023

Three species of the genus Phyllopodopsyllus T. Scott, 1906 (Copepoda: Harpacticoida: Tetragonicipitidae) were reported from the intertidal sand of Jeju Island and Busan in Korea, Phyllopdopsyllus kitazimai Karanovic, 2017, Phyllopodopsyllus saywakimi sp. nov. and Phyllopodopsyllus similis sp. nov. The latter two were described here. Phyllopodopsyllus saywakimi sp. nov. collected from only Udo, Jeju is similar to a Pacific species from Costa Rica, Phyllopodopsyllus ancylus Mielke, 1992. However, P. saywakimi sp. nov. differs from the Costa Rica species in the body size, the number of armatures on the antenna, the length ratio of endo- and exopod of the mandible, and the chaetotaxy of the fifth female pereiopod and the sixth male pereiopod. The second new species, P. similis sp. nov., found in Busan and several localities of Jeju, significantly resembles the species complex related to Phyllopodopsyllus aegypticus Nicholls, 1944, which is widely distributed in the Indian, Pacific, and Atlantic oceans. P. similis sp. nov. can be distinguished from the P. aegypticus complex by the body size, the shape of the rostrum, the type of armatures, the length ratio of rami and armatures, and the chaetotaxy of the female P5. In addition to the species descriptions, partial fragments of small and large subunit ribosomal RNA genes, cytochrome b, and cytochrome c oxidase subunit I were provided. Using molecular markers, a phylogenetic analysis was also performed to estimate the relationships among the three Korean species of Phyllopodopsyllus for the first time.
Altitudinal Patterns of Native and Invasive Alien Herbs along Roadsides in the Dayao Mountain National Nature Reserve, Guangxi, China
Authors: Li Bai , Ni Xinying , Zhao Caiyun
Published: January 2023

Invasive alien plants have rapidly established and spread in nature reserves via roads and now pose a threat to biodiversity. To understand the mechanism and distribution patterns of invasive alien herbs, we compared the altitude patterns of native and invasive alien herbs based on 105 plots in the Dayao Mountain National Nature Reserve. This study also compared the distribution patterns of new (introduced to China after 1900) and old (introduced to China before 1900) invasive alien herbs. In addition, we examined the effects of climatic factors and human activities on the distribution patterns of species richness. In our study, 151 native herbs species and 18 invasive alien herbs species were observed, of which 12 were new invasive alien herbs. Old invasive alien herbs occurred more frequently and occupied a wider range of altitudes than new invasive alien herbs. The richness of native herbs tended to decrease with increasing altitude, and the altitude patterns of the richness of all invasive herbs and new invasive alien herbs were hump-shaped. Based on an analysis using the linear mixed model, the results indicated that temperature was the main factor limiting the altitude patterns of native herbs, and that temperature and human activities were essential factors in the distribution and spread of all invasive alien herbs and new invasive alien herbs. The intensity of human interference is a crucial driver of the spread of new invasive alien herbs to higher altitudes.
Delayed Presentation of Functioning Metastasis in a Patient with Follicular Thyroid Carcinoma
Authors: Alexandre Maria Inês , Donato Sara , Vilar Helena , Leite Valeriano
Published: January 2023

Functioning metastases from differentiated thyroid carcinoma are rare and present a great therapeutic challenge. Here, we present an unusual case of a patient with metastatic thyroid carcinoma who developed a hyperthyroid state a few years after the diagnosis due to functioning metastases. Radioiodine treatment was effective in controlling the hyperthyroidism; however, it had no effect on tumor burden. By sharing our experience with this case, we hope to raise awareness for this rare condition and the ways to manage it.
Does Positive Thinking Help during Difficult Pandemic Times? The Role of Positive Orientation in the Relationship between Fear of COVID-19 and Perceived Stress
Authors: Dymecka Joanna , Gerymski Rafał , Machnik-Czerwik Anna , Rogowska Aleksandra M.
Published: January 2023

The COVID-19 pandemic has become a huge challenge for the modern world. How people perceive themselves and their coping abilities is important for their mental health and well-being. One of the traits that may be important in effectively coping with difficulties is positive orientation: a stable cognitive disposition that is the opposite of depression and is associated with a positive perception of oneself, one’s life, events, and the future. This study aimed to verify the role of positive orientation in the relationship between fear of COVID-19 and perceived stress. A sample of 907 Polish people took part in this study. FOC-6, P Scale, and PSS-10 questionnaires were used in the presented cross-sectional study. The analysis showed that women scored higher in fear of COVID-19 and perceived stress scores than men (Cohen’s d indicated a moderate effect). There was no significant difference in the levels of positive orientation. P Scale results were significantly related to fear of COVID-19 (small effect) only for the whole studied sample, and not for women and men considered separately. Fear of COVID-19 was positively related to the perceived stress score (moderate effect). Positive orientation was negatively related to the PSS-10 scores (also moderate effect) for all tested groups. Fear of COVID-19 and positive orientation were significant predictors of perceived stress scores. The mediation effect of positive orientation was statistically significant, but the effect size was marginally small. Positive orientation is an important predictor of perceived stress, which could be related to the COVID-19 pandemic. People with a positive orientation better cope with the challenges of the pandemic and are optimistic about the future. Working on positive orientation can improve well-being and reduce tension, which is extremely important in difficult pandemic times.
Morphological Characteristics of Catalyst Layer Defects in Catalyst-Coated Membranes in PEM Fuel Cells
Authors: Arcot Muneendra Prasad , Cronin Magnus , Fowler Michael , Pritzker Mark
Published: January 2023

Catalyst layer defects and irregularities in catalyst-coated membrane (CCM) electrodes affect the lifetime of polymer electrolyte membrane fuel cells (PEMFCs) during their operation. Thus, catalyst layer defects are important concerns for fuel cell manufacturers and prompt the development of quality control systems with the aim of fabricating defect-free electrodes. Consequently, the objective of this study is to gain a fundamental understanding of the morphological changes of real catalyst layer defects that have developed during CCM production. In this paper, missing catalyst layer defects (MCLD) formed during the decal transfer process are investigated through a nondestructive method using reflected light microscopy. The geometric features of the defects are quantified, and their growth is measured at regular time intervals from beginning-of-life (BOL) to end-of-life (EOL) until the OCV has dropped by 20% of its initial value as per a DOE-designed protocol. Overall, two types of degradation are observed: surface degradation caused by catalyst erosion and crack degradation caused by membrane mechanical deformation. Furthermore, catalyst layer defects formed during the decal transfer process were found to exhibit a higher growth rate at middle-of-life (MOL-1) and stabilize by EOL. This type of study will provide manufacturers with baseline information to allow them to select and reject CCMs, ultimately increasing the lifetime of fuel cell stacks.
Steady-State Vibration Level Measurement of the Five-Phase Induction Machine during Third Harmonic Injection or Open-Phase Faults
Authors: Muc Adam , Morawiec Marcin , Wilczyński Filip
Published: January 2023

Multiphase electric machines are increasingly used in various industries and for electromobility. Complex systems have been developed for the control and powering of multiphase machines, which require verification. The quality of control and the power supply of electric machines is usually evaluated by analyzing various electrical parameters. On the other hand, taking into account the fact that a motor is an electrical-mechanical object, its full diagnostics should also include the analysis of vibration signals to verify the operation of the motor as a mechanical device. In this paper, a sensorless control algorithm was studied and applied to a 5-phase induction motor. Various scenarios were considered; in particular, the operation of the studied motor in the absence of one or two phases and in the case of the introduction of the third harmonic to increase the torque was analyzed. In the scenarios considered, the motor was connected to another machine and operated with no load as well as with a preset load. The results obtained were analyzed in the time and frequency domain and were related to the standards used.
Green Diesel Production via Deoxygenation Process: A Review
Authors: Lucantonio Stefania , Di Giuliano Andrea , Rossi Leucio , Gallucci Katia
Published: January 2023

The environmental impact of traditional fuels and related greenhouse gas emissions (GHGE) has promoted policies driven towards renewable fuels. This review deals with green diesel, a biofuel obtained by catalytic deoxygenation of edible and non-edible biomasses. Green diesel, biodiesel, and petrodiesel are compared, with green diesel being the best option in terms of physical–chemical properties and reduction in GHGE. The deoxygenation process and the related types of catalysts, feedstocks, and operating conditions are presented. Reactor configurations are also discussed, summarizing the experimental studies. Several process simulations and environmental economic analyses—up to larger scales—are gathered from the literature that analyze the potential of green diesel as a substitute for petrodiesel. In addition, current industrial processes for green diesel production are introduced. Future research and development efforts should concern catalysts and the use of waste biomasses as feedstock, as well as the arrangement of national and international policies.
Decarbonisation Pathways for the Finishing Line in a Steel Plant and Their Implications for Heat Recovery Measures
Authors: Beck Anton , Unterluggauer Julian , Helminger Franz , Solís-Gallego Irene
Published: January 2023

Steel production is one of the biggest emitters of greenhouse gas in the industrial sector with about 8% of total global CO2 emissions. Although the majority of emissions can be attributed to primary steel production, there is also potential for reducing CO2 emissions in downstream steel processing. Large industrial furnaces, which are necessary for heating steel, are currently primarily fired with natural gas and by-product gases from primary steel production, offering great potential for heat recovery measures from exhaust gases. However, switching to alternative climate-neutral fuels could change this potential and thus jeopardize the economic viability of heat recovery measures. In the present work, it was therefore examined to what extent a change in energy sources in industrial furnaces affects the potential use of heat recovery in steel processing. For this purpose, an optimization model was used that takes into account heat recovery by means of direct heat transfer, heat pumps and heat distribution systems. Potential future changes in energy supply for industrial furnaces were examined using different storylines. Two different energy price scenarios were also considered to address uncertain developments in energy markets. The results show that heat recovery is a cost-effective and definitely recommendable measure. Switching to alternative fuels has little impact on the use of heat recovery. Electrification and thus the elimination of flue gas, on the other hand, greatly reduces the potential for heat recovery.
Observer-Based Robust Fault Predictive Control for Wind Turbine Time-Delay Systems with Sensor and Actuator Faults
Authors: Bououden Sofiane , Allouani Fouad , Abboudi Abdelaziz , Chadli Mohammed , Boulkaibet Ilyes , Barakeh Zaher Al , Neji Bilel , Ghandour Raymond
Published: January 2023

This paper presents a novel observer-based robust fault predictive control (OBRFPC) approach for a wind turbine time-delay system subject to constraints, actuator/sensor faults, and external disturbances. The proposed approach is based on an augmented state-space representation that contains state-space variables and estimation errors. The proposed augmented representation is then used to synthesize a robust predictive controller. In addition, an observer is developed and used to estimate both state variables and actuator/sensor faults. To ensure that the proposed approach has disturbance rejection capabilities, the disturbance estimates were merged with the prediction model. In addition, the disturbance rejection capabilities and fault tolerance were insured by formulating the control process as an optimization problem subject to constraints in terms of linear matrix inequalities (LMIs). As a result, the controller gains are acquired by solving an LMI problem to guarantee input-to-state stability in the presence of sensor and actuator faults. A simulation example is conducted on a nonlinear wind turbine (1 MW) model with 3 blades, a horizontal axis, and upwind variable speed subject to actuator/sensor faults in the pitch system. The results demonstrate the ability of the proposed method in dealing with nonlinear systems subject to external disturbances and keeping the control performance acceptable in the presence of actuator/sensor faults.
Final Energy Consumption—Growth Nexus in Romania Versus the European Union: A Sectoral Approach Using Neural Network
Authors: Soava Georgeta , Mehedintu Anca
Published: January 2023

The energy crisis caused first by the COVID-19 pandemic and continued by the Russo-Ukrainian War has demonstrated that energy is a determining factor in the conduct of activities of any state. Several studies have examined the relationship between energy consumption and economic growth, developing various theories, but there is no consensus. This study investigates relationships by analyzing several regression models and choosing the fittest. Then, the verification of its validity is performed through the neural network, which gives greater credibility to the results obtained. Furthermore, using a structural analysis, the investigation is expanded to ranking the impact of sector-specific energy consumption on economic growth. The research includes data from 1995 to 2020 for the European Union (EU) and Romania. The results indicate that short-term energy consumption can have a positive or negative impact on economic growth, both in the EU and Romania. The structural analysis highlights the direct and indirect effects, with different intensities, of sector-specific energy consumption on economic growth. This study is interested primarily in the conditions of uncertainty caused by the COVID-19 pandemic and the Russo-Ukrainian War, to enable the EU and each member state to take effective energy policy measures to ensure their energy security.
Efficiency of Applying the Nyquist and V.M. Popov Criteria for Stability Analysis of Linearized Automatic Control Systems in Electromechanics and Power Engineering
Authors: Kodkin Vladimir , Anikin Alexander , Kuznetsova Ekaterina
Published: January 2023

It is well known that all real automatic control systems (ACS) are significantly nonlinear systems. Along with this, in engineering analysis the simplifications of their mathematic description, that is, linearization, are inevitable. These simplifications naturally introduce errors into engineering calculations. For the most complicated calculation of stability conditions, these errors can be very significant, since they lead to not only incorrect forecast of the ACS condition but also to the operating inability of the designed ACS. Most often, engineers simplify nonlinear structures in their calculations. In other words, they linearize these structures according to one of the numerous approaches which have been developed over 100 years. Calculation methods for linear ACS, in particular the Nyquist criterion, can be applied to the already linearized structure for the calculation of ACS stability condition. In this article, the authors suppose that for electromechanical and power ACS it would be more effective to widely use the absolute stability criterions or hyperstability which were developed when solving the “Aizerman problem” by V.M. Popov in the 1960s of the 20th century. This solution is not used as widely as the Nyquist criterion despite its outstanding qualities. In this article, the assumption was made that it is effective to apply the stability criterion of nonlinear ACS for the linearized models of nonlinear systems. Otherwise speaking, it is effective to use the criterion of V.M. Popov and its interpretation according to the logarithmic frequency characteristics of the ACS proposed earlier by the authors of the article. The authors provide simple and illustrative examples and give the results of the simulation.
Thermal Management Techniques for Lithium-Ion Batteries Based on Phase Change Materials: A Systematic Review and Prospective Recommendations
Authors: Shi Hong , Cheng Mengmeng , Feng Yi , Qiu Chenghui , Song Caiyue , Yuan Nenglin , Kang Chuanzhi , Yang Kaijie , Yuan Jie , Li Yonghao
Published: January 2023

Thermal management systems for lithium-ion batteries based on the cooling and heating of phase change materials have become a popular research topic. However, the low thermal conductivity, flame resistance, high and low temperature adaptability of phase change materials, as well as the thermal runaway mechanisms and lightweight design of phase change material-based systems remain to be explored. The aim of this paper is to conduct a publication-wide macro bibliometric review on thermal management systems for lithium-ion batteries based on phase change material to date. Total of 583 associated publications were retrieved from the Web of Science Core Collection database for the period 2006–2022. A bibliometric study was conducted through the visualization software VOSviewer. The findings were derived from annual publication trends, geographical and institutional distribution, authors and their collaborative networks, keyword network analysis and analysis of highly cited publications as well as reference co-citation analysis. The findings provide a comprehensive overview of the evolution of research hotspots in the field and can help researchers who would like to work in the field to quickly grasp the research frontiers and the overall picture. Furthermore, some suggestions for future work are summarized.
Root-Growth-Related MaTCP Transcription Factors Responsive to Drought Stress in Mulberry
Authors: Wei Wuqi , He Jinzhi , Luo Yiwei , Yang Zhen , Xia Xiaoyu , Han Yuanxiang , He Ningjia
Published: January 2023

Root growth regulation plays a crucial role in the acclimatization of plants to their surroundings, but the molecular mechanisms underlying this process remain largely uncertain. Teosinte branched1/cycloidea/proliferating cell factor (TCP) transcription factors are crucial elements linking together plant growth and development, phytohormone signaling, and stress response. In this study, 15 TCP transcription factors were identified in the mulberry (Morus alba) genome. Gene structure, conserved motif, and phylogenetic analyses revealed the conservation and divergence of these MaTCPs, thus providing insights into their functions. A promoter analysis uncovered distinct numbers and compositions of cis-elements in MaTCP gene promoter regions that may be connected to reproductive growth and phytohormone and stress responses. An expression pattern analysis of the 15 MaTCP genes in mulberry roots indicated that transcriptional levels of MaTCP2, MaTCP4-1, MaTCP8, MaTCP9-1, and MaTCP20-2 are correlated with root development. As revealed by changes in their expressions after drought treatment, these five MaTCP genes are involved in root growth and may increase mulberry tolerance to drought. Our findings lay the foundation for future functional studies of these genes.
Genetic Influences on Fetal Alcohol Spectrum Disorder
Authors: Sambo Danielle , Goldman David
Published: January 2023

Fetal alcohol spectrum disorder (FASD) encompasses the range of deleterious outcomes of prenatal alcohol exposure (PAE) in the affected offspring, including developmental delay, intellectual disability, attention deficits, and conduct disorders. Several factors contribute to the risk for and severity of FASD, including the timing, dose, and duration of PAE and maternal factors such as age and nutrition. Although poorly understood, genetic factors also contribute to the expression of FASD, with studies in both humans and animal models revealing genetic influences on susceptibility. In this article, we review the literature related to the genetics of FASD in humans, including twin studies, candidate gene studies in different populations, and genetic testing identifying copy number variants. Overall, these studies suggest different genetic factors, both in the mother and in the offspring, influence the phenotypic outcomes of PAE. While further work is needed, understanding how genetic factors influence FASD will provide insight into the mechanisms contributing to alcohol teratogenicity and FASD risk and ultimately may lead to means for early detection and intervention.
Genome-Wide Analysis of Aquaporin Gene Family in Triticum turgidum and Its Expression Profile in Response to Salt Stress
Authors: Yaghobi Mahnaz , Heidari Parviz
Published: January 2023

During the response of plants to water stresses, aquaporin (AQP) plays a prominent role in membrane water transport based on the received upstream signals. Due to the importance of the AQP gene family, studies have been conducted that investigate the function and regulatory system of these genes. However, many of their molecular aspects are still unknown. This study aims to carry out a genome-wide investigation of the AQP gene family in Triticum turgidum using bioinformatics tools and to investigate the expression patterns of some members in response to salt stress. Our results show that there are 80 TtAQP genes in T. turgidum, which are classified into four main groups based on phylogenetic analysis. Several duplications were observed between the members of the TtAQP gene family, and high diversity in response to post-translational modifications was observed between TtAQP family members. The expression pattern of TtAQP genes disclosed that these genes are primarily upregulated in response to salt stress. Additionally, the qPCR data revealed that TtAQPs are more induced in delayed responses to salinity stress. Overall, our findings illustrate that TtAQP members are diverse in terms of their structure, regulatory systems, and expression levels.
Critical Analysis of the Materials Used by the Venetian Artist Guido Cadorin (1892–1976) during the Mid-20th Century, Using a Multi-Analytical Approach
Authors: Morales Toledo Erik Guillermo , Raicu Teodora , Falchi Laura , Barisoni Elisabetta , Piccolo Matteo , Izzo Francesca Caterina
Published: January 2023

The present study sought to expand on and confirm the already available information on the painting materials used by the Venetian artist Guido Cadorin (1892–1976). A multi-analytical approach was employed in the study of six tempera grassa easel paintings and one casein tempera on a panel signed by the artist and belonging to the International Gallery of Modern Art Ca’ Pesaro in Venice, Italy, which dated from 1921 to 1951. The aim of the research was to identify the painting materials, observe the evolution of the color palette through time and assess the state of conservation. Non-invasive imaging and/or spectroscopic techniques were employed, such as hyperspectral imaging spectroscopy (HSI) and Raman spectroscopy. Microsamples were also collected from the edges and detached areas of the canvases that were studied through three non-destructive techniques, namely optical microscopy (OM), energy dispersive x-ray fluorescence spectrometry (EDXRF) and attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR), and one destructive technique, namely gas chromatography-mass spectrometry (GC/MS). The results allowed the inference of the color palette used to render the artist’s paints, composition of the preparation layer, and characterization of the binding media and varnish layers. Moreover, the state of conservation of the artworks was determined. Thus, the outcome of this research enriches the painter’s profile and might aid the International Gallery of Modern Art Ca’ Pesaro in Venice, Italy in the planning of future conservation treatments in accordance with the guidelines of good practices in art conservation.
Psychological Status of Men Who Have Sex with Men during COVID-19: An Online Cross-Sectional Study in Western China
Authors: Lin Bing , Liu Jiaxiu , He Wei , Pan Haiying , Zhong Xiaoni
Published: January 2023

Background: The psychological status of men who have sex with men (MSM) as a vulnerable population during COVID-19 is worthy of attention. However, studies of Chinese MSM are limited. The aim of this study was to investigate the psychological status and influencing factors of MSM population and to provide a scientific basis for this group to actively respond to public health emergencies. Methods: From June to September 2020, we conducted an online survey. MSMs were recruited through collaboration with non-governmental organizations (NGOs) and peer recommendations. The variables we collected included four aspects: demographic and HIV-related characteristics; COVID-19-related knowledge, attitudes, and behaviors; COVID-19-related risk perception; epidemic exposure. The psychological status was assessed by the Psychological Questionnaire for Emergent Events of Public Health (PQEEPH), which defined the psychological status as five primary disorders: depression, neurasthenia, fear, anxiety, and hypochondria. Multivariate logistic regression was used to explore the influences of COVID-19-related factors on the psychological status. Results: We surveyed 412 MSM online during COVID-19. The five psychological status of high-risk states accounted for 16.99% (depression), 14.08% (neurasthenia), 16.75% (fear), 16.50% (anxiety), and 18.20% (hypochondria), respectively. Not being well-informed about the causes of COVID-19 (p = 0.020) and having experienced epidemic exposure (p = 0.006) were able to promote the occurrence of depression. Lack of knowledge of the curative effect of COVID-19 has a higher risk of occurrence neurasthenia (p < 0.001). Being afraid of the novel coronavirus (p < 0.001) promoted fear. The perceived prevalence of the epidemic (p = 0.003), being more susceptible to COVID-19 (p < 0.001), and not being well-informed about the causes of COVID-19 (p = 0.005) had a positive effect on anxiety. Considering that the epidemic was not effectively controlled (p = 0.017), being more susceptible to COVID-19 (p < 0.001) was a contributing factor to the hypochondria. Conclusions: The incidence of psychological disorders in Chinese MSM was higher than that in other groups during COVID-19. Factors associated with COVID-19 may cause a range of mental health problems in this population. Greater attention should be paid to the mental health status of special populations during the epidemic, and effective preventive education and intervention measures should be taken.
Diagnosis and Treatment of Lower Extremity Arterial Disease—A Survey among Family Medicine Trainees in Poland
Authors: Danieluk Aleksandra , Niemcunowicz-Janica Anna , Windak Adam , Chlabicz Sławomir
Published: January 2023

Guidelines point to the ankle-brachial index (ABI) as a non-invasive tool for the initial diagnosis of lower extremity artery disease (LEAD). Questions have been raised whether primary practices should perform ABI. An online questionnaire was distributed among family medicine trainees in two academic centers in Poland. The questionnaire aimed to establish their knowledge about LEAD management and their opinion on the usefulness of ABI measurement and other LEAD diagnostic methods in primary care. ABI measurement was found either very or moderately useful in LEAD diagnosis by 94.5% of the respondents. Among the three most important elements of LEAD management, lifestyle changes, secondary prevention of atherosclerosis and exercise treatment were chosen, respectively, by 98.6%, 83.6% and 72.6% of them. ABI was seen as a useful diagnostic method at the primary care by 74% of the participants; however, 82.2% of them do not have access to ABI measurement in their workplace. The residents have good knowledge of the diagnostic methods of LEAD and consider ABI measurement as useful in LEAD diagnosis. However, most of them do not have access to ABI measurements in their clinical practices. Future discussion and potential financial changes will be needed for the introduction of ABI measurements into Polish primary care.
Neighborhood Environment and Affective Walking Experience: Cluster Analysis Results of a Virtual-Environment-Based Conjoint Experiment
Authors: Liao Bojing , Li Xiang
Published: January 2023

There is empirical evidence that neighborhood environment characteristics influence individuals’ self-reported affective walking experiences. However, much of the research investigates residents’ affective walking experiences at the neighborhood level using revealed-preference methodologies, making it difficult to identify the separate impacts of characteristics. In addition, empirical studies have not shown enough evidence that individuals from different sociodemographic backgrounds have distinct affective walking experiences. Therefore, the objective of this paper is to explain how different groups of people perceive the characteristics of a neighborhood differently. To do this, this study conducts a conjoint experiment employing videos of virtual environments involving a sample of 295 respondents. Using a latent-class regression model and a multinomial logit model, we are able to determine how individuals and groups perceive neighborhood characteristics differently based on their different emotions. The results somewhat confirmed the findings of the empirical research, indicating that land use mix, connectivity, road size, open space, and greenery are related to a positive walking experience. The level of affective walking experience that individuals associate with neighborhood environmental characteristics is, however, considerably variable. Therefore, our results show that open space and road width are crucial for a walkable neighborhood since they are most helpful to individuals’ subjective well-being.
Emotion Recognition from Large-Scale Video Clips with Cross-Attention and Hybrid Feature Weighting Neural Networks
Authors: Zhou Siwei , Wu Xuemei , Jiang Fan , Huang  Qionghao , Huang Changqin
Published: January 2023

The emotion of humans is an important indicator or reflection of their mental states, e.g., satisfaction or stress, and recognizing or detecting emotion from different media is essential to perform sequence analysis or for certain applications, e.g., mental health assessments, job stress level estimation, and tourist satisfaction assessments. Emotion recognition based on computer vision techniques, as an important method of detecting emotion from visual media (e.g., images or videos) of human behaviors with the use of plentiful emotional cues, has been extensively investigated because of its significant applications. However, most existing models neglect inter-feature interaction and use simple concatenation for feature fusion, failing to capture the crucial complementary gains between face and context information in video clips, which is significant in addressing the problems of emotion confusion and emotion misunderstanding. Accordingly, in this paper, to fully exploit the complementary information between face and context features, we present a novel cross-attention and hybrid feature weighting network to achieve accurate emotion recognition from large-scale video clips, and the proposed model consists of a dual-branch encoding (DBE) network, a hierarchical-attention encoding (HAE) network, and a deep fusion (DF) block. Specifically, the face and context encoding blocks in the DBE network generate the respective shallow features. After this, the HAE network uses the cross-attention (CA) block to investigate and capture the complementarity between facial expression features and their contexts via a cross-channel attention operation. The element recalibration (ER) block is introduced to revise the feature map of each channel by embedding global information. Moreover, the adaptive-attention (AA) block in the HAE network is developed to infer the optimal feature fusion weights and obtain the adaptive emotion features via a hybrid feature weighting operation. Finally, the DF block integrates these adaptive emotion features to predict an individual emotional state. Extensive experimental results of the CAER-S dataset demonstrate the effectiveness of our method, exhibiting its potential in the analysis of tourist reviews with video clips, estimation of job stress levels with visual emotional evidence, or assessments of mental healthiness with visual media.
Effectiveness of Blood Flow Restriction on Functionality, Quality of Life and Pain in Patients with Neuromusculoskeletal Pathologies: A Systematic Review
Authors: Reina-Ruiz Álvaro Jesús , Martínez-Cal Jesús , Molina-Torres Guadalupe , Romero-Galisteo Rita-Pilar , Galán-Mercant Alejandro , Carrasco-Vega Elio , González-Sánchez Manuel
Published: January 2023

Background: Blood flow restriction is characterized as a method used during exercise at low loads of around 20–40% of a repetition maximum, or at a low-moderate intensity of aerobic exercise, in which cuffs that occlude the proximal part of the extremities can partially reduce arterial flow and fully restrict the venous flow of the musculature in order to achieve the same benefits as high-load exercise. Objective: The main objective of this systematic literature review was to analyze the effects of BFR intervention on pain, functionality, and quality of life in subjects with neuromusculoskeletal pathologies. Methods: The search to carry out was performed in PubMed, Cochrane, EMBASE, PEDro, CINHAL, SPORTDiscus, Trip Medical Database, and Scopus: “kaatsu” OR “ischemic training” OR “blood flow restriction” OR “occlusion resistance training” OR “vascular occlusion” OR “vascular restriction”. Results: After identifying 486 papers and eliminating 175 of them due to duplication and 261 after reading the title and abstract, 50 papers were selected. Of all the selected articles, 28 were excluded for not presenting a score equal to or higher than 6 points on the PEDro scale and 8 for not analyzing the target outcome variables. Finally, 14 papers were selected for this systematic review. Conclusions: The data collected indicate that the blood flow restriction tool is a therapeutic alternative due to its effectiveness under different exercise modalities. The benefits found include decreases in pain thresholds and improvement in the functionality and quality of life of the neuro-musculoskeletal patient during the first six weeks. However, the results provided by this tool are still not clear for medium- and long-term interventions.
Proportion of Chinese Children and Adolescents Meeting 24-Hour Movement Guidelines and Associations with Overweight and Obesity
Authors: Sun Yi , Liu Yuan , Yin Xiaojian , Li Ming , Zhang Ting , Zhang Feng , Guo Yaru , Sun Pengwei
Published: January 2023

Background: Since there is little knowledge about the 24-hour movement behaviors of Chinese children and adolescents, the purposes of this study were to investigate the proportion of Chinese children and adolescents meeting the 24-Hour Movement Guidelines and to further evaluate its relationship with overweight and obesity. Methods: A total of 440 children and adolescents aged 7–18 years from 7 cities in China were selected to measure physical activity using accelerometers, and sleep (SLP) and screen time (ST) using questionnaires. The data were analyzed with the independent T-test, Mann–Whitney U test, Cox–Stuart test, chi-square test, and logistic regression. Results: The proportion of Chinese children and adolescents meeting the overall 24-Hour Movement Guidelines was 7.3%. Boys (11.8%) were higher than girls (3.4%) (p < 0.001) and showed a downward trend with age (Ptrend = 0.03). The rates of overweight and obesity among children and adolescents who met the ST, MVPA + ST, ST + SLP, and MVPA + SLP + ST guidelines were 39%, 15%, and 36%, and 25% did not meet any guidelines. The rates of overweight and obesity among those who met 1, 2, and 3 guidelines were lower than the rate among those who did not meet any guidelines (odds ratio (OR) = 0.51, 95% confidence interval (CI): 0.22–1.17; OR = 0.32, 95% CI: 0.13–0.77; OR = 0.23, 95% CI: 0.07–0.81) and showed a decreasing trend (Ptrend = 0.006). Conclusions: The proportion of Chinese children and adolescents meeting the overall 24-Hour Movement Guidelines was low. The rate of overweight and obesity among children and adolescents who met the overall 24-Hour Movement Guidelines was the lowest compared with the rates among those who met any one or two. There was a dose–response relationship between the number of guidelines met and the overweight and obesity rate.
Altitude and Its Association with Low Birth Weight among Children of 151,873 Peruvian Women: A Pooled Analysis of a Nationally Representative Survey
Authors: Hernández-Vásquez Akram , Bartra Reátegui Alicia , Vargas-Fernández Rodrigo
Published: January 2023

The aim of this study was to determine the relationship between the altitude of residence and the low birth weight (LBW) of the children of pregnant Peruvian women using a nationally representative database. An analysis of individual-level data from the last 13 years (from 2009 to 2021) of the Demographic and Family Health Survey was performed. The outcome variable was LBW, defined as birth weight less than 2500 g, while the independent variable was the altitude of residence in meters above sea level (masl). To estimate the association between the two variables, the crude and adjusted generalized linear model of the Poisson family with a log link was used along with crude and adjusted prevalence ratios, which were estimated with their respective 95% confidence interval. A total of 151,873 women aged 15–49 years were included between 2009 and 2021. The pooled proportion of LBW was 7.0%. As the main finding, the children of mothers residing at an altitude from 2500 to 3499 masl and ≥3500 masl had a higher probability of LBW. It was found that the children of mothers residing at an altitude above 2500 masl were more likely to have LBW. Our results will help to strengthen the cultural practice of maternal health care and increase its coverage in women residing in high-altitude regions.
Structural Insights of the DciA Helicase Loader in Its Relationship with DNA
Authors: Cargemel Claire , Baconnais Sonia , Aumont-Nicaise Magali , Noiray Magali , Maurin Lia , Andreani Jessica , Walbott Hélène , Le Cam Eric , Ochsenbein Françoise , Marsin Stéphanie , Quevillon-Cheruel Sophie
Published: January 2023

DciA is the ancestral bacterial replicative helicase loader, punctually replaced during evolution by the DnaC/I loaders of phage origin. DnaC helps the helicase to load onto DNA by cracking open the hexameric ring, but the mechanism of loading by DciA remains unknown. We demonstrate by electron microscopy, nuclear magnetic resonance (NMR) spectroscopy, and biochemistry experiments that DciA, which folds into a KH-like domain, interacts with not only single-stranded but also double-stranded DNA, in an atypical mode. Some point mutations of the long α-helix 1 demonstrate its importance in the interaction of DciA for various DNA substrates mimicking single-stranded, double-stranded, and forked DNA. Some of these mutations also affect the loading of the helicase by DciA. We come to the hypothesis that DciA could be a DNA chaperone by intercalating itself between the two DNA strands to stabilize it. This work allows us to propose that the direct interaction of DciA with DNA could play a role in the loading mechanism of the helicase.
Antitumoral Activity of Leptocarpha rivularis Flower Extracts against Gastric Cancer Cells
Authors: Carrasco Nicolás , Garrido Maritza , Montenegro Iván , Madrid Alejandro , Hartley Ricardo , González Iván , Rubilar Mariaignacia , Villena Joan , Valenzuela-Valderrama Manuel
Published: January 2023

Leptocarpha rivularis is a native South American plant used ancestrally by Mapuche people to treat gastrointestinal ailments. L. rivularis flower extracts are rich in molecules with therapeutic potential, including the sesquiterpene lactone leptocarpin, which displays cytotoxic effects against various cancer types in vitro. However, the combination of active molecules in these extracts could offer a hitherto unexplored potential for targeting cancer. In this study, we investigated the effect of L. rivularis flower extracts on the proliferation, survival, and spread parameters of gastric cancer cells in vitro. Gastric cancer (AGS and MKN-45) and normal immortalized (GES-1) cell lines were treated with different concentrations of L. rivularis flower extracts (DCM, Hex, EtOAc, and EtOH) and we determined the changes in proliferation (MTS assay, cell cycle analysis), cell viability/cytotoxicity (trypan blue exclusion assay, DEVDase activity, mitochondrial membrane potential MMP, and clonogenic ability), senescence (β-galactosidase activity) and spread potential (invasion and migration assays using the Boyden chamber approach) in all these cells. The results showed that the DCM, EtOAc, and Hex extracts display a selective antitumoral effect in gastric cancer cells by affecting all the cancer parameters tested. These findings reveal an attractive antitumoral potential of L. rivularis flower extracts by targeting several acquired capabilities of cancer cells.