bluuebunny/crossref_metadata_2025 huggingface dataset:
Results of 110 IVORY® prostheses for trapeziometacarpal osteoarthritis with a minimum follow-up of 10 years
Authors: Tchurukdichian Alain , Guillier David , Moris Vivien , See Leslie-Ann , Macheboeuf Yvan
Published: January 2020

The IVORY® prosthesis is a total trapeziometacarpal joint prosthesis used for the treatment of symptomatic trapeziometacarpal osteoarthritis. The aim of this prospective study was to evaluate its long-term outcomes with a minimum follow-up of 10 years. From 2004 to 2007, 110 trapeziometacarpal prostheses (95 patients) were implanted. The implant survival curve was constructed using the Kaplan–Meier method. Five patients were lost from follow-up and two died. Six prosthesis (5.5%) were removed after dislocation or fracture of the trapezium. The survival rate of the prosthesis was 95%. After 10 years, the mean visual analogue pain score was 0.24/10 and the key-pinch force was similar to the other hand. Dislocations occurred for eight implants (7.3%). No radiological loosening was noted. In conclusion, the long-term results with the IVORY® prosthesis are very satisfactory in terms of pain relief, function and survival.

Level of evidence: II
Scaphometacarpal arthroplasty: a report of ten cases of trapeziometacarpal prosthesis and trapeziectomy revision
Authors: Chiche Leo , Lamarre Herve , Barbary Stephane , Teissier Jacques
Published: January 2020

Loosening of the trapezial component is a main cause of failure of trapeziometacarpal prostheses. This report presents the preliminary results of scaphometacarpal prostheses used for revision of trapeziometacarpal prostheses and failed trapeziectomies. A retrospective multicentre study was conducted on ten patients. Four had revision surgery after failure of trapeziometacarpal prostheses, five after trapeziectomy and one after a trapezial silicone implant. Pain, mobility, strength, QuickDASH, satisfaction score and radiographs were assessed. Nine patients were assessed (one was lost to follow-up), with a mean follow-up of 34 months. The mean pain score was 1.2/10, Quick-DASH was 39 and opposition according to the Kapandji score was 8.6. Strength was 3 kg for key pinch and 13.6 kg for power grip. One failure was observed, with early loosening of the scaphoid cup. Scaphometacarpal arthroplasty is a reliable medium-term solution for revision of the loosening of a trapeziometacarpal prosthesis with trapezial damage and for failed trapeziectomy.

Level of evidence: IV
Different Strategies to Obtain Corn (Zea mays L.) Germ Extracts with Enhanced Antioxidant Properties
Authors: Burlini Ilaria , Grandini Alessandro , Tacchini Massimo , Maresca Immacolata , Guerrini Alessandra , Sacchetti Gianni
Published: January 2020

Maize ( Zea mays L.) germs are by-products from the milling industry. The objective of this work was to compare the phenolic and lipophilic antioxidant fractions of yellow and white corn varieties, provided by Corn Valley S.r.l. (Piumbega, Mantova, Italy) and among the raw materials most processed by the company. The phenolic fraction, extracted with ultrasound-assisted extraction, alone and in combination with chemical and enzymatic hydrolyses, was analyzed with high-performance thin-layer chromatography and reversed-phase high-performance liquid chromatography-diode array detector. Among the various extraction techniques used, the combination of sonication and alkaline hydrolysis proved to be an effective method for the extraction of phenolic compounds from yellow and white germs, with the highest ferulic acid concentrations (636.54 ± 3.71 and 569.23 ± 1.69 mg FA/g dried extract, respectively), total phenolic contents (844.5 ± 64.6 and 742.8 ± 15.44 mg gallic acid equivalents/g dried extract, respectively), and the best antioxidant activity (14.33 ± 0.48 and 11.41 ± 1.1 µg/mL, respectively). The lipophilic fraction, extracted using supercritical carbon dioxide was analyzed by gas chromatography-mass spectrometry. The unsaponifiable fractions were found to be 2.41% ± 0.24% in yellow corn and 1.85% ± 0.08% in white corn; β-sitosterol, campesterol, and stigmasterol were identified as the main phytosterols characterizing both lipophilic extracts which showed the most effective antioxidant activity (1.29 ± 0.26 mg/mL and 1.33 ± 0.21 mg/mL, respectively) compared with the control. Finally, the phenolic and lipophilic extracts obtained from maize by-products may be reintroduced into the health-oriented market as extracts enriched of high-added value biomolecules with antioxidant activity both as active molecules and as additives of natural origin.
A Multifactorial Approach to Overuse Running Injuries: A 1-Year Prospective Study
Authors: Winter Sara C. , Gordon Susan , Brice Sara M. , Lindsay Daniel , Barrs Sue
Published: January 2020

Background:

Because of the complex and multifaceted nature of running injuries, a multifactorial approach when investigating running injuries is required.

Hypothesis:

Compared with uninjured runners, injured runners would exhibit different running biomechanics, display more fatigue changes, and would run a greater weekly running volume; more injured runners would also report having a previous injury.

Study Design:

Prospective cohort study.

Level of Evidence:

Level 4.

Methods:

At commencement of the study, data were collected on demographics, anthropometrics, training history, previous injury history, and center-of-mass accelerations during a long-distance overground run. Participants completed weekly training diaries and were monitored for 1 year for an injury.

Results:

A total of 76 runners completed the study, with 39 (22 male; 17 female) reporting an injury. Compared with male uninjured runners, male injured runners were heavier and ran a greater weekly distance. Male runners (injured and uninjured) exhibited increases in mediolateral center-of-mass accelerations during the run. Compared with female uninjured runners, female injured runners were heavier, ran with longer flight times and lower step frequencies, and more of them had reported an injury in the previous year and had increased speed training in the weeks prior to injury. Over 60% of male injured runners and over 50% of female injured runners had increased their weekly running distance by >30% between consecutive weeks at least once in the 4 weeks prior to injury.

Conclusion:

Factors that may be related to injury for male runners include being heavier, running a greater weekly distance, and exhibiting fatigue changes in mediolateral center-of-mass accelerations. Factors that may be related to injury for female runners include being heavier, having an injury in the previous year, running with longer flight times and lower step frequencies, and increasing speed training prior to injury. Increases in weekly running distance in 1 consecutive week (particularly >30%) needs to be monitored in training, and this along with the other factors found may have contributed to injury development.

Clinical Relevance:

This study found that multiple factors are related to running injuries and that some factors are sex specific. The findings can aid in injury prevention and management.
Application of Clinical Scores in the Differential Diagnosis of Chronic Rhinosinusitis With Nasal Polyps in a Chinese Population
Authors: Xu Qingqing , Du Kun , Zheng Ming , Duan Su , Jia Shuangshuang , Chen Hui , Wang Xiangdong , Zhang Luo
Published: January 2020

Background

Eosinophilic (Eos) and non-eosinophilic (non-Eos) chronic rhinosinusitis with nasal polyps (CRSwNP) react differently to clinical treatment, with eosinophilic chronic rhinosinusitis with nasal polyps (Eos CRSwNP) being more likely to recur after surgery.

Objective

To explore the clinical value of the visual analog scale (VAS), nasal endoscopy score (Lund–Kennedy, L–K), computed tomography (CT) score (Lund–Mackay scoring system, L–M), and blood Eos percentage in the differential diagnosis of Eos CRSwNP and non-Eos CRSwNP.

Methods

Ninety-nine patients with CRSwNP were enrolled in this study and assigned to 2 groups (Eos CRSwNP and non-Eos CRSwNP). The blood Eos percentage and VAS, L–K, and L–M scores in the 2 groups of patients were compared. A receiver operating characteristic (ROC) was used to assess the usefulness of VAS, L–K, and L–M scores for differentiating Eos CRSwNP and non-Eos CRSwNP.

Results

There were significantly differences between the Eos CRSwNP group and non-Eos CRSwNP group in the following scores: blood Eos percentage, mean VAS score, olfaction/VAS, general discomfort/L–K, edema score/L–K, olfactory cleft (OC) score via endoscopy, mean L–M score, anteriorethmoid sinus score, posterior ethmoid sinus score, sphenoid sinus score, frontal sinus score, and OC score via CT. An ROC analysis showed that blood Eos percentage had the highest area under the ROC curve (AUC) value (0.749); however, several other scores (olfaction score/VAS, edema score/L–K, and mean L–M score) also had high AUC values. The combination of olfaction score/VAS and blood Eos percentage had the highest clinical convenience score as well as high sensitivity and specificity. A combination of cutoff values for the 2 predictors (blood Eos percentage ≥3.85%, olfaction score/VAS score ≥3) showed a sensitivity of 75.5% and a specificity of 78.0%.

Conclusion

The olfaction score/VAS score and the blood Eos percentage can be combined to differentiate Eos CRSwNP from non-Eos CRSwNP in a Chinese population.
Partial Preservation of the Inferior Turbinate in Endoscopic Medial Maxillectomy: A Computational Fluid Dynamics Study
Authors: Saibene Alberto M. , Felisati Giovanni , Pipolo Carlotta , Bulfamante Antonio Mario , Quadrio Maurizio , Covello Vanessa
Published: January 2020

Background

Endoscopic medial maxillectomy (EMM) is a workhorse for multiple sinonasal conditions. To reduce its burden on the sinonasal physiology, several modified EMM (M-EMM) have been proposed. Objective: In order to provide a theoretical basis for EMM and its modifications, this study introduces a computational fluid dynamics (CFD) model, based on a time-resolved direct numerical simulation, describing EMM and assessing the role of the M-EMM in preserving the overall fluid dynamics of the sinonasal cavities.

Methods

A normal sinonasal CT scan was converted into a geometrical model and used as a reference; 2 anatomies were then created by virtual surgery, mimicking EMM and M-EMM, with the latter sparing the anterior portion of inferior turbinate and medial maxillary sinus wall. The airflow was simulated in the models via the OpenFOAM CFD software and compared in terms of flow rate, mean and fluctuating velocity, vorticity, and turbulent structures.

Results

The analysis shows that EMM induces a massive flow rate increase in the operated side, which becomes less obvious in the M-EMM model. In contrast to M-EMM, EMM induces higher velocity fields that reach the maxillary sinus. Velocity and vorticity fluctuations are negligible in the baseline model, but become increasingly evident and widespread in the M-EMM and EMM models.

Conclusions

A significant disruption of the nasal fluid dynamics is observed in EMM, while M-EMM minimizes variations and reduces interference with nasal air conditioning. Our analysis provides insights into the pathophysiology of radical sinus surgery and provides a theoretical basis for the ability of M-EMM to reduce the temporary surgery-related changes on both healthy and operated sides.
Safety and efficacy of axicabtagene ciloleucel in refractory large B-cell lymphomas
Authors: Riedell Peter A. , Bishop Michael R.
Published: January 2020

Aggressive large B-cell lymphomas represent a diverse population of diseases that are typically treated with anti-CD20 based immunochemotherapy. While this treatment is effective for a large proportion of patients, those that become refractory to induction therapy or experience disease relapse suffer an inferior overall prognosis, and novel treatment options are needed. Adoptive T-cell immunotherapy in the form of chimeric antigen receptor (CAR) T-cell therapy is one of the most revolutionary breakthroughs in the past several decades for the treatment of relapsed/refractory aggressive large B-cell lymphomas. Based on data from the pivotal ZUMA-1 study, axicabtagene ciloleucel (axi-cel) became the first-in-class anti-CD19 directed CAR T-cell therapy approved for patients with diffuse large B-cell lymphoma and other aggressive B-cell lymphoma variants. In this review, we provide an overview of CAR T-cell therapy, including its biology, manufacturing, and treatment course. In addition, we highlight the available efficacy data, review pertinent safety concerns, including cytokine release syndrome and neurologic toxicity, as well as provide an overview of emerging therapeutic strategies in the cellular therapy arena.
On the effectiveness of ventilation to mitigate the damage of spherical membrane vessels subjected to internal detonations
Authors: Hernandez Francisco , Zhang Xihong , Hao Hong
Published: January 2020

This article conducts a comparative study on the effectiveness of ventilation to mitigate blasting effects on spherical chambers subjected to internal detonations of high explosives through finite element analysis using the software package AUTODYN. Numerical simulations show that ventilation is ineffective in mitigating the damage of spherical chambers subjected to internal high explosives explosions because the chamber response is mainly described by high-frequency membrane modes. Openings do not reduce the chamber response despite they can reduce the blast overpressure after the chamber reaches its peak response. Worse still, openings lead to stress concentration, which weakens the structure. Therefore, small openings may reduce the capacity of the chamber to resist internal explosions. In addition, because large shock waves impose the chamber to respond to a reverberation frequency associated with the re-reflected shock wave pulses, secondary re-reflected shock waves can govern the chamber response, and plastic/elastic resonance can occur to the chamber. Simulations show that the time lag between the first and the second shock wave ranges from 3 to 7 times the arrival time of the first shock wave, implying that the current simplified design approach should be revised. The response of chambers subjected to eccentric detonations is also studied. Results show that due to asymmetric explosions, other membrane modes may govern the chamber response and causes localized damage, implying that ventilation is also ineffective to mitigate the damage of spherical chambers subjected to eccentric detonations.
Short tau inversion recovery MRI of Modic changes: a reliability study
Authors: Kristoffersen Per Martin , Vetti Nils , Storheim Kjersti , Bråten Lars Christian , Rolfsen Mads Peder , Assmus Jörg , Espeland Ansgar
Published: January 2020

Background

Limited reliability data exist for evaluation of spinal edema changes on magnetic resonance imaging (MRI) with short tau inversion recovery (STIR) sequences.

Purpose

To assess the inter-observer reliability for evaluation of STIR signal increase related to Modic changes (MCs) on MRI of the lumbar spine.

Material and Methods

We prospectively included 120 patients imaged to confirm their eligibility for the AIM (Antibiotics In Modic changes) trial. Three experienced radiologists independently evaluated MCs on T1-/T2-weighted fast spin-echo images and subsequently MC-related STIR signal increases. Inter-observer reliability was analyzed at four endplates (L4–S1) by calculating kappa values and means of differences with 95% limits of agreement.

Results

Overall agreement (mean Fleiss’ kappa for all endplates and observers) was very good for presence of STIR signal increase (0.86), and moderate for its categorized height (0.51), anteroposterior extent (0.48), and volume (0.56). For height of region with STIR signal increase measured in % points of vertebral body height, the largest mean of differences was 6.9 and widest range for limits of agreement was ±22.3 for all endplates combined. The corresponding numbers were 11.2 ± 34.5 for anteroposterior extent of the STIR signal increase measured in % points of anteroposterior endplate diameter and 0.9 ± 7.6 for its maximum measured intensity on a % point scale (0% = normal vertebral marrow intensity, 100% = cerebrospinal fluid intensity).

Conclusion

Inter-observer reliability was very good for the presence and intensity of MC-related STIR signal increases, and moderate for their size.
The ‘tapestry’ of bricolage: Extending interdisciplinary approaches to psycho-spiritual self-care research
Authors: Devenish-Meares Reverend Peter
Published: January 2020

Interdisciplinary psycho-spiritual research into workplace stress and self-care is scant noting the fact that negative self-talk and harsh self-judgement stymie the search for inner meaning and self-care. To address this, this article uses an intuitive and reflection-oriented methodology to research self-care choices for the stressed and suffering worker. In particular, it breaks new ground because no workplace-based applied psycho-spiritual research uses bricolage, let alone the heuristic inquiry process which gives expression to it. Bricolage is a tapestry of ideas, themes and possibilities cobbled together to produce creative outcomes. It adapts and co-opts whatever information from whichever discipline that is necessary. This approach appears well suited to the subjective, intuitive nature of workplace stress and suffering and especially where interdisciplinary approaches to self-care are warranted. The heuristic inquiry process which is used for the first time for workplace self-care works intentionally with interior resources that may be unknown or fragmented and dialogues sensitively with inner ‘rules’ or patterns that may have become problematic. Incubation and reflective illumination produce tacit knowledge to augment healing intuition. This process is illustrated by an example. It is about a less intense focus that actively encourages tender, ‘standing apart’ from symptoms so as to allow perspectives to arise and the intuiting of transformative possibilities. From this, self-compassion, humility and meaningful detachment are re-affirmed as ways to pay self-kindly attention and address self-criticism and self-blame. The contribution of the study is threefold. First, it extends bricolage to workplace self-care by considering inner resistance and negative self-talk, both barriers to self-care. Second, it affirms the heuristic inquiry process as an intuitive method for self-care research. Third, and paradoxically, it shows that self-engagement, in a compassionate yet less intense way, can lead to self-care transformation. Finally, limitations to the study and possibilities for future research are discussed.
Evaluating the Research Performance of Islamabad-Based Higher Education Institutes
Authors: Javed Yasir , Ahmad Shakil , Khahro Shabir Hussain
Published: January 2020

Research discovers new knowledge, ideas, and technologies essential in driving the future of society and humanity. Without research, a relevant and modern country cannot exist. Similarly, the research output of universities and degree-awarding institutes (DAIs) plays a significant role in higher education and development of any country. Research fosters professional excellence in faculty, important for delivering outstanding student education and training. Therefore, this research is an evaluation of the research output of higher education DAIs in the capital of Pakistan. This research was conducted in four steps: (a) data collection: where the data were extracted from Scopus for the years 2008–2017; (b) data cleansing and labeling: which included removal of nonrequired contents and labeling of nonnumeric data to meaningful classes; (c) feature selection: useful features according to proposed research questions were selected; and (d) data analysis: data were analyzed according to research questions and results obtained from experiments with the help of statistical tools. It is concluded that with only 1% of country’s population and around 11% of Higher Education Commission’s (HEC) recognized universities geographically located at Islamabad Capital Territory, Islamabad-based universities and DAIs contributed 34% of the total publications of Pakistan produced during the study time period. This shows Islamabad-based universities and DAIs standing at the national level. The public sector universities shared the major portion of total publications of the studied universities. Private sector universities and DAIs shared 8% of the total publications.
Does Attractiveness Lead to or Follow From Occupational Success? Findings From German Associational Football
Authors: Meier Henk Erik , Mutz Michael
Published: January 2020

Prior research has provided evidence that attractiveness is associated with work-related advantages. It is less clear, however, whether attractiveness is an antecedent or a consequence of professional success. To answer this question, associational football in Germany is used as an exemplifying case. Portrait pictures of German football players were retrieved, one picture from a very early career stage and one from a very late one. Attractiveness of these portraits was assessed by the “truth of consensus” method. Panel regression models are applied to analyze changes in attractiveness and relate these changes to professional success. Findings show that success as a footballer cannot be predicted with attractiveness at early career stages. Instead, the increase of attractiveness over time is more pronounced among very successful players. It is thus concluded that successful individuals are not more attractive in the very beginning, but improve their appearance throughout their careers.
The Sensitivity of Firms’ Investment to Uncertainty and Cash Flow: Evidence From Listed State-Owned Enterprises and Non-State-Owned Enterprises in China
Authors: Khan Muhammad Arif , Qin Xuezhi , Jebran Khalil , Rashid Abdul
Published: January 2020

This study examines the association between various uncertainties and corporate investment and further investigates this association between state-owned enterprises (SOEs) and non-state-owned enterprises (non-SOEs). Moreover, this study analyzes the indirect effects of uncertainty on corporate investment through cash flow. The current research uses an unbalanced panel data of Chinese nonfinancial listed firms for the period 1999–2016. To control endogeneity issues, this study applies a robust two-step system generalized method of moments (GMM) technique to estimate the model. Empirical findings indicate that market-based and firm-specific uncertainties have positive effects, whereas economic policy and CAPM-based uncertainties have negative effects on corporate investment. Furthermore, results indicate that the effects of market-based, CAPM-based, and firm-specific uncertainties (economic policy uncertainty) were less (more) prominent for SOEs. Additional analyses show that cash flow stimulates the effect of firm-specific uncertainty on SOEs’ investment, whereas it weakens the influence of CAPM-based uncertainty (economic policy uncertainty) on investment of non-SOEs (SOEs). Moreover, cash flow attenuates the market uncertainty effect on investment.
Main and Moderating Effects of an Online Transition Curriculum on Career Readiness
Authors: Lombardi Allison , Rifenbark Graham , Tarconish Emily , Volk Daniel , Monahan Jessica , Buck Andrew , Izzo Margo , Murray Alexa
Published: January 2020

In this study, students with and without disabilities ( n = 816) in general and special education settings in Grades 9 through 12 were participants in a quasi-experimental design to examine the main effects of an online curriculum intervention with transition-related content on career readiness. Setting and teacher characteristics were examined for moderating effects. Results showed a main effect across settings (general and special education, self-contained, resource rooms) as well as differentiating effects based on teacher fidelity and the number of lessons taught. Implications are discussed with regard to the need for further examination of these contextual factors in high schools so all students, with and without disabilities, are provided career readiness opportunities.
Successful prosthetic rehabilitation and gait analysis of individual with bilateral transtibial amputation: A case study with comparison to able-bodied gait
Authors: Mohanty Rajesh Kumar , Kumar Jay Prakash , Rout Somanath , Das Sakti Prasad
Published: January 2020

Background:

Intensive rehabilitation of individuals with bilateral lower limb loss poses a great challenge to both rehabilitation team and amputees themselves due to unavailability of a sound leg to provide stability in standing and gait. Although gait characteristics of individuals with unilateral transtibial amputations are well documented in the literature, very less is known about those with bilateral limb loss.

Aim:

To examine the gait characteristics of an individual with bilateral transtibial amputation (BTA) and its comparison with an able-bodied (AB). This study also provides a real-life presentation of successful prosthetic rehabilitation.

Case content and methodology:

Temporal–spatial, kinematic and kinetic gait parameters were analysed for a 45-year-old male individual with traumatic BTA using prosthesis in a motion analysis laboratory setting with force platform (BTS P-6000) and cameras with reflective markers (BTS SMART-DX6000).

Findings and conclusion:

Variances in many temporal–spatial, kinematic and kinetic parameters were observed. The findings of temporal–spatial parameters revealed that the individual with BTA walked with slower speed, lower cadence, shorter step lengths and wider step width compared to that of AB. Ankle dorsiflexion, stance knee flexion and swing hip hiking were reduced in an individual with BTA compared to AB. In kinetics, he demonstrated low peak ankle muscle power, increased muscle power amplitudes and phase duration at the hip and knee joints compared to AB individual. The combination of an intensive prosthetic rehabilitation led to completely independent and remarkable degree of functional ambulation.
The floating flail chest: Acute management of an injury combination of the floating shoulder and flail chest
Authors: Cunningham Brian P , Bosch Liam , Swanson David , McLemore Ryan , Rhorer Anthony S , Parikh Harsh R , Albersheim Melissa , Ortega Gilbert
Published: January 2020

Background/purpose:

The combination of ipsilateral floating shoulder and flail chest is a unique injury pattern that has not been previously described in the literature. We termed the injury pattern floating flail chest (FFC). The purpose of this study was to evaluate the effect of operative treatment of the shoulder girdle component to overall hospital length of stay (LOS).

Methods:

Forty-one patients were enrolled between two level I trauma centers identifying with a combination ipsilateral floating shoulder and flail chest injury, 23 treated with operative stabilization and 18 treated non-operatively. This retrospective cohort study evaluated the overall LOS and intensive care unit (ICU) days.

Results:

The operative group had decreased overall LOS (10.1 vs. 19.8 days, p = 0.02) and decreased ICU days (3.4 vs. 10.3, p = 0.04).

Conclusion:

This study describes a unique injury pattern that combines the floating shoulder and flail chest, FFC. Our study suggests that operative treatment of the shoulder girdle may decrease both overall LOS and ICU days in patients with FFC.
Deep vein thrombosis in the lower extremities after femoral neck fracture: A retrospective observational study
Authors: Fu Ya-Hui , Liu Ping , Xu Xin , Wang Peng-Fei , Shang Kun , Ke Chao , Fei Chen , Yang Kun , Zhang Bin-Fei , Zhuang Yan , Zhang Kun
Published: January 2020

Purpose:

The actual incidence of deep vein thrombosis (DVT) in femoral neck fractures is underestimated. This study aimed to investigate the incidence of DVT in the lower extremities after femoral neck fracture before and after operation.

Methods:

The clinical data of patients with femoral neck fractures treated at Xi’an Honghui Hospital between July 1, 2016, and December 31, 2018, were collected. The patients were examined with ultrasonography before and after operation and divided into thrombosis and non-thrombosis groups according to their ultrasonographic results. The incidence of DVT was reported as a percentage.

Results:

The incidence rates of preoperative and postoperative DVT were 32% and 56%, respectively. DVT on the uninjured side constituted 45% of all preoperative DVT and 43% of all postoperative DVT. Peripheral DVT constituted 90% and 84% of all preoperative and postoperative DVT, respectively. Diabetes was an independent risk factor of preoperative DVT. Blood loss was an independent risk factor of postoperative DVT, and open reduction and internal fixation surgical procedure was independent protective factor of postoperative DVT as compared with hemiarthroplasty and total hip replacement.

Conclusions:

The incidence rates of preoperative and postoperative DVT in the patients with femoral neck fracture were high, and orthopedists should pay more attention to DVT as a complication.
The role of the immune system in driving neuroinflammation
Authors: Ní Chasaide Caitlín , Lynch Marina A
Published: January 2020

Neuroinflammation is now recognised as an important contributory factor in the progression of Alzheimer’s disease and probably also in the early stages of the disease. It is likely that this derives largely from aberrant activation of microglia, the resident mononuclear phagocytes of the brain. These cells are responsible for physiological immune surveillance and clearance of pathogens in the central nervous system, but evidence indicates that in Alzheimer’s disease, microglial function is compromised, and this contributes to the pathology. It is unclear what factors cause the inappropriate activation of the microglia in Alzheimer’s disease, but one contributor may be infiltrating peripheral immune cells and these include macrophages and T cells. It has been suggested that both cell types modulate the phenotype of microglia, highlighting the importance of crosstalk between the innate and adaptive immune system in Alzheimer’s disease. This review outlines our current knowledge of how cells of the peripheral immune system, specifically macrophages and T cells, may modulate microglial phenotype in the context of Alzheimer’s disease and considers the impact on their function, especially phagocytic capacity.
Impact of graft sources on immune reconstitution and survival outcomes following allogeneic stem cell transplantation
Authors: Ando Taiki , Tachibana Takayoshi , Tanaka Masatsugu , Suzuki Taisei , Ishiyama Yasufumi , Koyama Satoshi , Ogusa Eriko , Numata Ayumi , Matsumoto Kenji , Kanamori Heiwa , Nakajima Hideaki
Published: January 2020

We evaluated the kinetics of immune reconstitution (IR) after allogeneic hematopoietic cell transplantation (HSCT) and analyzed the clinical effect of IR on posttransplant outcomes. Absolute lymphocyte and its subset counts were measured using flow cytometry on days 28, 100, 180, 365, and 730 after transplantation in 358 adult patients who underwent HSCT between 2009 and 2017. On day 100 after HSCT, 310 surviving patients were analyzed. Bone marrow transplantation (BMT), peripheral blood stem cell transplantation (PBSCT), and cord blood transplantation (CBT) were performed in 119, 55, and 136 patients, respectively. Mature B-cell and differentiated natural killer (NK) cell subset counts significantly increased after CBT. The 2-year overall survival (OS), nonrelapse mortality (NRM), cumulative incidence of relapse, and chronic GVHD in BMT, PBSCT, and CBT were 62%, 67%, and 76% (P = .021); 17%, 17%, and 13% (P = .82); 33%, 40%, and 27% (P = .063); and 43%, 45%, and 28% (P = .025), respectively. Multivariate analysis showed that higher CD16+CD57− NK cell counts correlated with lower disease relapse, whereas higher CD20+ B-cell counts correlated with lower NRM. OS-favoring factors were higher CD16+CD57− NK cell count (hazard ratio, 0.36; 95% confidence interval, 0.22-0.60; P < .001) and CD20+ B-cell count (hazard ratio, 0.53; 95% confidence interval, 0.30-0.93; P < .001) and lower Disease Risk/HCT-Specific Comorbidity index score. Collective contribution of graft source-specific and event-related immune reconstitution might yield better posttransplant outcomes in CBT.
NGF (−198C > T, Ala35Val) and p75NTR (Ser205Leu) gene mutations are associated with liver function in different histopathological profiles of the patients with chronic viral hepatitis in the Brazilian Amazon
Authors: Pereira Leonn Mendes Soares , da Silva Graça Amoras Ednelza , da Silva Conde Simone Regina Souza , Demachki Sâmia , dos Santos Eduardo José Melo , Lima Sandra Souza , Ishak Ricardo , Rosário Vallinoto Antonio Carlos
Published: January 2020

Backgrounds

Neural growth factor (NGF) is a neurotrophin that can interact with the p75NTR receptor and initiate a cascade of reactions that determines cell survival or death, and both are associated with the physiology of liver tissue. Single nucleotide polymorphisms (SNPs) in the NGF and p75NTR genes have been investigated in different pathologies; however, there are no studies that have analyzed their biological roles in the hepatic microenvironment. In the present study, we evaluated the impact of SNPs in these genes on the maintenance of liver function at different stages of inflammation and fibrosis in patients with chronic viral liver disease in the Brazilian Amazon.

Methods

The SNPs -198C > T, Arg80Gln, Val72Met, Ala35Val, Ala18Ala and Ser205Leu were genotyped by real-time PCR in samples from patients with chronic viral hepatitis stratified by stage of inflammation and liver fibrosis. Histopathological, viral load (VL), liver enzyme and comorbidities data were obtained from updated medical records. Other aspects were highlighted by applied epidemiological questionnaires.

Results

The -198C/T and Ala35Val polymorphisms in NGF were associated with changes in histopathological profiles, VL and liver enzymes. Ser205Leu polymorphism in p75NTR was associated only with changes in VL and liver enzymes. Polymorphic frequencies were variable among different ethnic populations, mainly for biologically relevant polymorphisms. A multifactorial network of interactions has been established based on genetic, virological, behavioral and biochemical aspects.

Conclusion

Mutations in the NGF (−198C > T, Ala35Val) and p75NTR (Ser205Leu) genes, within the list of multifactorial aspects, are associated with liver function in different histopathological profiles of patients with chronic viral liver disease in the Brazilian Amazon.
The association of CASC16 variants with breast Cancer risk in a northwest Chinese female population
Authors: Zuo Xiaoxiao , Wang Huanhuan , Mi Yin , Zhang Yue , Wang Xiaofei , Yang Ya , Zhai Suna
Published: January 2020

Purpose

Genetic variants play a critical role in the development of breast cancer. This investigation aimed to explore the association between CASC16 polymorphisms and breast cancer susceptibility.

Methods

We conducted a case-control study of 681 patients and 680 healthy individuals to investigate the correlation of five SNPs with breast cancer in a Northwest Chinese female population. Odds ratios (OR) and 95% confidence intervals (CIs) were used to assess the association.

Results

Our study found that rs4784227 and rs12922061 were significantly related to an increased susceptibility to breast cancer (OR 1.22, p = 0.022; OR 1.21, p = 0.026). While rs3803662 was a protective role in breast cancer risk (OR 0.69, p = 0.042). Stratified analyses indicated that rs4784227 and rs12922061 would increase breast cancer susceptibility at age >  50 years. Rs3803662 was a reduced factor of breast cancer risk by age ≤ 50 years. Rs4784227 was significantly increased risk of breast cancer in stage III/IV. The rs45544231 and rs3112612 had a protective effect on breast cancer with tumor size > 2 cm. Rs4784227 and rs12922061 could enhance breast cancer risk in lymph node metastasis positive individuals. CASC16 rs12922061 and rs4784227 polymorphisms correlated with an increased risk of breast cancer in BMI >  24 kg/m2. Haplotype analyses revealed that Grs45544231 Trs12922061 Ars3112612 and Grs45544231 Crs12922061 Ars3112612 haplotypes decreased breast cancer risk.

Conclusion

Our study revealed that CASC16 genetic variants were significantly related to breast cancer susceptibility, which might give scientific evidence for exploring the molecular mechanism of breast cancer.
Characterization of cyclitic membranes by ultrabiomicroscopy in patients with pars planitis
Authors: Concha del Río Luz Elena , Duarte González Gonzalo Alejandro , Mayorquín Ruiz Mariana , Arellanes-García Lourdes
Published: January 2020

Background

In previous studies, authors use ultrasound biomicroscopy (UBM) to analyze the characteristics of cyclitic membranes and the associated complications in patients with pars planitis. However, there are no reports regarding the prevalence of cyclitic membranes or complications at diagnosis and during follow-up.

Purpose

To describe the characteristics and complications of cyclitic membranes, as determined by UBM in patients with pars planitis using AVISO-S™ (Quantel Medical) equipment with a 50-MHz linear probe with a focus at the pars plana.

Design

This retrospective study reviewed UBM images of patients diagnosed with pars planitis, from the Inflammatory Eye Disease Clinic in Mexico City from January 2010 to June 2016.

Results

Cyclitic membranes were observed in the first UBM image in 67 eyes (56.7%) and during follow-up in 81 eyes (68.62%). In 67 eyes (82.71%), the cyclitic membranes extended through one or two quadrants. Extension toward the posterior lens capsule was recognized in 15 eyes (18.52%) and extension toward the peripheral retina in 12 eyes (14.81%). Complications included ciliary body detachments in 10 eyes (12.35%) and peripheral retinal traction in 8 eyes (9.88%).

Conclusions

UBM is a valuable tool for the diagnosis of cyclitic membranes at admittance and during follow-up of patients with pars planitis; it helps the clinician to detect this complication early.
Unlocking the transcriptomic potential of formalin-fixed paraffin embedded clinical tissues: comparison of gene expression profiling approaches
Authors: Turnbull Arran K. , Selli Cigdem , Martinez-Perez Carlos , Fernando Anu , Renshaw Lorna , Keys Jane , Figueroa Jonine D. , He Xiaping , Tanioka Maki , Munro Alison F. , Murphy Lee , Fawkes Angie , Clark Richard , Coutts Audrey , Perou Charles M. , Carey Lisa A. , Dixon J. Michael , Sims Andrew H.
Published: January 2020

Background

High-throughput transcriptomics has matured into a very well established and widely utilised research tool over the last two decades. Clinical datasets generated on a range of different platforms continue to be deposited in public repositories provide an ever-growing, valuable resource for reanalysis. Cost and tissue availability normally preclude processing samples across multiple technologies, making it challenging to directly evaluate performance and whether data from different platforms can be reliably compared or integrated.

Methods

This study describes our experiences of nine new and established mRNA profiling techniques including Lexogen QuantSeq, Qiagen QiaSeq, BioSpyder TempO-Seq, Ion AmpliSeq, Nanostring, Affymetrix Clariom S or U133A, Illumina BeadChip and RNA-seq of formalin-fixed paraffin embedded (FFPE) and fresh frozen (FF) sequential patient-matched breast tumour samples.

Results

The number of genes represented and reliability varied between the platforms, but overall all methods provided data which were largely comparable. Crucially we found that it is possible to integrate data for combined analyses across FFPE/FF and platforms using established batch correction methods as required to increase cohort sizes. However, some platforms appear to be better suited to FFPE samples, particularly archival material.

Conclusions

Overall, we illustrate that technology selection is a balance between required resolution, sample quality, availability and cost.
m6Acorr: an online tool for the correction and comparison of m6A methylation profiles
Authors: Li Jianwei , Huang Yan , Cui Qinghua , Zhou Yuan
Published: January 2020

Background

The analysis and comparison of RNA m6A methylation profiles have become increasingly important for understanding the post-transcriptional regulations of gene expression. However, current m6A profiles in public databases are not readily intercomparable, where heterogeneous profiles from the same experimental report but different cell types showed unwanted high correlations.

Results

Several normalizing or correcting methods were tested to remove such laboratory bias. And m6Acorr, an effective pipeline for correcting m6A profiles, was presented on the basis of quantile normalization and empirical Bayes batch regression method. m6Acorr could efficiently correct laboratory bias in the simulated dataset and real m6A profiles in public databases. The preservation of biological signals was examined after correction, and m6Acorr was found to better preserve differential methylation signals, m6A regulated targets, and m6A-related biological features than alternative methods. Finally, the m6Acorr server was established. This server could eliminate the potential laboratory bias in m6A methylation profiles and perform profile–profile comparisons and functional analysis of hyper- (hypo-) methylated genes based on corrected methylation profiles.

Conclusion

m6Acorr was established to correct the existing laboratory bias in RNA m6A methylation profiles and perform profile comparisons on the corrected datasets. The m6Acorr server is available at http://www.rnanut.net/m6Acorr. A stand-alone version with the correction function is also available in GitHub at https://github.com/emersON106/m6Acorr.
A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms
Authors: Abdul Hamid Aizat Iman , Nakusi Laurence , Givskov Mickael , Chang Young-Tae , Marquès Claire , Gueirard Pascale
Published: January 2020

Background

Staphylococcus aureus is a human pathogen that is a common cause of nosocomial infections and infections on indwelling medical devices, mainly due to its ability to shift between the planktonic and the biofilm/sessile lifestyle. Biofilm infections present a serious problem in human medicine as they often lead to bacterial persistence and thus to chronic infections. The immune responses elicited by biofilms have been described as specific and ineffective. In the few experiments performed in vivo, the importance of neutrophils and macrophages as a first line of defence against biofilm infections was clearly established. However, the bilateral interactions between biofilms and myeloid cells remain poorly studied and analysis of the dynamic processes at the cellular level in tissues inoculated with biofilm bacteria is still an unexplored field. It is urgent, therefore, to develop biologically sound experimental approaches in vivo designed to extract specific immune signatures from the planktonic and biofilm forms of bacteria.

Results

We propose an in vivo transgenic mouse model, used in conjunction with intravital confocal microscopy to study the dynamics of host inflammatory responses to bacteria. Culture conditions were created to prepare calibrated inocula of fluorescent planktonic and biofilm forms of bacteria. A confocal imaging acquisition and analysis protocol was then drawn up to study the recruitment of innate immune cells in the skin of LysM-EGFP transgenic mice. Using the mouse ear pinna model, we showed that inflammatory responses to S. aureus can be quantified over time and that the dynamics of innate immune cells after injection of either the planktonic or biofilm form can be characterized. First results showed that the ability of phagocytic cells to infiltrate the injection site and their motility is not the same in planktonic and biofilm forms of bacteria despite the cells being considerably recruited in both cases.

Conclusion

We developed a mouse model of infection to compare the dynamics of the inflammatory responses to planktonic and biofilm bacteria at the tissue and cellular levels. The mouse ear pinna model is a powerful imaging system to analyse the mechanisms of biofilm tolerance to immune attacks.
Sexual dimorphism following in vitro ischemia in the response to neurosteroids and mechanisms of injury
Authors: Altaee Raeed , Gibson Claire L.
Published: January 2020

Background

Cerebral ischemic stroke is a significant cause of morbidity and mortality. Sex differences exist following stroke in terms of incidence, symptoms, outcomes and response to some treatments. Importantly, molecular mechanisms of injury, activated following ischemia may differ between the sexes and if so may account, at least in part, for sex differences seen in treatment response. Here we aimed to determine, using single-sex organotypic hippocampal slice cultures, whether the effectiveness of a potential treatment option, i.e. sex steroids, exhibited any sexual dimorphism and whether sex affected the mechanisms of apoptosis activated following ischemia.

Results

Following exposure to ischemia, male-derived tissue exhibited higher levels of cell death than female-derived tissue. Various sex steroid hormones, i.e. progesterone, allopregnanolone, and estradiol, were protective in terms of reducing the amount of cell death in male- and female-derived tissue whereas medoxyprogesterone acetate (MPA) was only protective in female-derived tissue. The protective effect of progesterone was abolished in the presence of finasteride, a 5α-reductase inhibitor, suggesting it was largely mediated via its conversion to allopregnanolone. To test the hypothesis that sex differences exist in the activation of specific elements of the apoptotic pathway activated following ischemia we administered Q-VD-OPH, a caspase inhibitor, or PJ34, an inhibitor of poly (ADP ribose) polymerase (PARP). Caspase inhibition was only effective, in terms of reducing cell death, in female-derived tissue, whereas PARP inhibition was only protective in male-derived tissue. However, in both sexes, the protective effects of progesterone and estradiol were not observed in the presence of either caspase or PARP inhibition.

Conclusions

Sex differences exist in both the amount of cell death produced and those elements of the cell death pathway activated following an ischemic insult. There are also some sex differences in the effectiveness of steroid hormones to provide neuroprotection following an ischemic insult—namely MPA was only protective in female-derived tissue. This adds further support to the notion sex is an important factor to consider when investigating future drug targets for CNS disorders, such as ischemic stroke.