bluuebunny/crossref_metadata_2025 huggingface dataset:
Insight into Point Defects and Complex Defects in β-Mo2C and Carbide Evolution from First Principles
Authors: Guo Jing , Feng Yunli , Tang Cong , Wang Li , Qing Xiaoliang , Yang Qingxiang , Ren Xuejun
Published: July 2022

In this paper, first principles method was adopted to investigate the point defects, Vanadium-related defects and defect combinations (vacancy (V), substitutional (S) and/or interstitial (I)) in molybdenum β-Mo2C and explore the use of first principles calculation data in analysing the link between different carbides and the effects of doping elements. Supercell models with different defect types were established and optimized, and the formation energy data of defects was developed. The structure evolution during the optimization process is analysed in detail to establish the main characteristics of changes and the relevant electronic properties. The data for different types of intrinsic defects and combined defects complexes was developed and key results is analysed. The results show that carbon vacancy (VC) is stable but does not inevitably exist in pure β-Mo2C. Interstitial site II is a very unstable position for any type of atoms (Mo, V and C), and analysis of the structure evolution shows that the atom always moves to the interface area near the interstitial site I between two layers. In particular, a C atom can expand the lattice structure when it exists between the layer interfaces. One type of the defects studied, the substitution of Mo with V (designated as ‘SV-Mo’), is the most stable defect among all single point defects. The data for defect complexes shows that the combination of multiple SV-Mo defects in the super cell being more stable than the combination of other defects (e.g., ‘VMo+IC’, ‘SV-Mo+VC’). The data with increasing SV-Mo in (Mo, V)2C system is developed, and typical data (e.g., formation energy) for Mo-rich carbides and V carbides are correlated and the potential of the data in analysing transition of different carbides is highlighted. The relevance of using first principles calculation data in the studying of V-doping and the complex carbides (V- and Mo-rich carbides) evolution in different materials systems and future focus of continuous work is also discussed.
Fabrication of Mn-Doped SrTiO3/Carbon Fiber with Oxygen Vacancy for Enhanced Photocatalytic Hydrogen Evolution
Authors: Hu Qi , Niu Jiantao , Zhang Ke-Qin , Yao Mu
Published: July 2022

With carbon fiber, it is difficult to load semiconductor photocatalysts and easy to shed off thanks to its smooth surface and few active groups, which has always been a problem in the synthesis of photocatalysts. In the study, SrTiO3 nanoparticles were loaded onto the Tencel fibers using the solvothermal method, and then the Tencel fibers were carbonized at a high temperature under the condition of inert gas to form carbon fibers, thus SrTiO3@CF photocatalytic composite materials with solid core shell structure were prepared. Meanwhile, Mn ions were added into the SrTiO3 precursor reagent in the solvothermal experiment to prepare Mn-doped Mn-SrTiO3@CF photocatalytic composite material. XPS and EPR tests showed that the prepared Mn-SrTiO3@CF photocatalytic composite was rich in oxygen vacancies. The existence of these oxygen vacancies formed oxygen defect states (VOs) below the conduction band, which constituted the capture center of photogenerated electrons and significantly improved the photocatalytic activity. The photocatalytic hydrogen experimental results showed that the photocatalytic hydrogen production capacity of Mn-SrTiO3@CF composite material with 5% Mn-doped was six times that of the SrTiO3@CF material, and the doping of Mn ions not only promoted the red shift of the light absorption boundary and the extension to visible light, but also improved the separation and migration efficiency of photocarriers. In the paper, the preparation method solves the difficulty of loading photocatalysts on CF and provides a new design method for the recycling of catalysts, and we improve the hydrogen production performance of photocatalysts by Mn-doped modification and the introduction of oxygen vacancies, which provides a theoretical method for the practical application of hydrogen energy.
The Use of Miniature Specimens to Determine Local Properties and Fracture Behavior of LPBF-Processed Inconel 718 in as-Deposited and Post-Treated States
Authors: Dzugan Jan , Seifi Mohsen , Rund Martin , Podany Pavel , Grylls Richard , Lewandowski John J.
Published: July 2022

This paper summarizes the assessment of directional anisotropy in local mechanical properties for Laser Powder Bed Fusion (LPBF) IN-718 bulk samples via the use of miniature samples excised from the bulk for both as-deposited and post-treated states. The quasi-static tensile properties at room temperature are investigated at several different locations along the build direction and at different orientations for both considered states. A comparison between the excised miniature tensile specimens and standard-sized sample results have also been conducted and exhibit very good agreement. Significant anisotropy is present in mechanical properties at different build heights for the as-deposited state, while the post-treated material exhibited more homogenous properties, both along the height and for different sampling orientations. However, significant reductions (e.g., >30%) in the strength (Yield, UTS) along with a significant increase in the reduction in area at fracture is found for post-processed materials. Metallography and fractography analyses were conducted in order to begin to determine the source(s) of this anisotropy for the as-deposited state.
On the Morphological Deviation in Additive Manufacturing of Porous Ti6Al4V Scaffold: A Design Consideration
Authors: Naghavi Seyed Ataollah , Wang Haoyu , Varma Swastina Nath , Tamaddon Maryam , Marghoub Arsalan , Galbraith Rex , Galbraith Jane , Moazen Mehran , Hua Jia , Xu Wei , Liu Chaozong
Published: July 2022

Additively manufactured Ti scaffolds have been used for bone replacement and orthopaedic applications. In these applications, both morphological and mechanical properties are important for their in vivo performance. Additively manufactured Ti6Al4V triply periodic minimal surface (TPMS) scaffolds with diamond and gyroid structures are known to have high stiffness and high osseointegration properties, respectively. However, morphological deviations between the as-designed and as-built types of these scaffolds have not been studied before. In this study, the morphological and mechanical properties of diamond and gyroid scaffolds at macro and microscales were examined. The results demonstrated that the mean printed strut thickness was greater than the designed target value. For diamond scaffolds, the deviation increased from 7.5 μm (2.5% excess) for vertical struts to 105.4 μm (35.1% excess) for horizontal struts. For the gyroid design, the corresponding deviations were larger, ranging from 12.6 μm (4.2% excess) to 198.6 μm (66.2% excess). The mean printed pore size was less than the designed target value. For diamonds, the deviation of the mean pore size from the designed value increased from 33.1 μm (−3.0% excess) for vertical struts to 92.8 μm (−8.4% excess) for horizontal struts. The corresponding deviation for gyroids was larger, ranging from 23.8 μm (−3.0% excess) to 168.7 μm (−21.1% excess). Compressive Young’s modulus of the bulk sample, gyroid and diamond scaffolds was calculated to be 35.8 GPa, 6.81 GPa and 7.59 GPa, respectively, via the global compression method. The corresponding yield strength of the samples was measured to be 1012, 108 and 134 MPa. Average microhardness and Young’s modulus from α and β phases of Ti6Al4V from scaffold struts were calculated to be 4.1 GPa and 131 GPa, respectively. The extracted morphology and mechanical properties in this study could help understand the deviation between the as-design and as-built matrices, which could help develop a design compensation strategy before the fabrication of the scaffolds.
Study of Morphological, Structural, and Strength Properties of Model Prototypes of New Generation TRISO Fuels
Authors: Kenzhina Inesh , Blynskiy Petr , Kozlovskiy Artem , Begentayev Meiram , Askerbekov Saulet , Zaurbekova Zhanna , Tolenova Aktolkyn
Published: July 2022

The purpose of this work is to characterize the morphological, structural, and strength properties of model prototypes of new-generation TRi-structural ISOtropic particle fuel (TRISO) designed for Generation IV high-temperature gas reactors (HTGR-type). The choice of model structures consisting of inner pyrolytic carbon (I-PyC), silicon carbide (SiC), and outer pyrolytic carbon (O-PyC) as objects of research is motivated by their potential use in creating a new generation of fuel for high-temperature nuclear reactors. To fully assess their full functional value, it is necessary to understand the mechanisms of resistance to external influences, including mechanical, as in the process of operation there may be external factors associated with deformation and leading to the destruction of the surface of fuel structures, which will critically affect the service life. The objective of these studies is to obtain new data on the fuel properties, as well as their resistance to external influences arising from mechanical friction. Such studies are necessary for further tests of this fuel on corrosion and irradiation resistance, as closely as possible to real conditions in the reactor. The research revealed that the study samples have a high degree of resistance to external mechanical influences, due to the high strength of the upper layer consisting of pyrolytic carbon. The presented results of the radiation resistance of TRISO fuel testify to the high resistance of the near-surface layer to high-dose irradiation.
Cyclic Performance of Structural Steels after Exposure to Various Heating–Cooling Treatments
Authors: Du Peng , Liu Hongbo , Xu Xuchen
Published: July 2022

The cyclic performance of structural steels after exposure to various elevated temperatures and cooling-down methods was experimentally investigated in this paper. Four types of frequently used structural steels were tested including Chinese mild steel Grade Q235, Chinese high-strength steel Grade Q345, and Chinese stainless steel Grade S304 and S316. A total of eighty specimens were prepared using three different heating–cooling processes before being subjected to cyclic loads. The post-fire basic features and hysteretic performances of the four types of structural steels exposed to various target temperatures (100–1000 °C), heat soak times (30 min or 180 min) and cooling-down methods (natural air or water) were recorded and discussed. The results show that all the tested structural steels prepared using different heating–cooling treatments exhibited proper ductility and energy dissipation capacity, while the heat soak times and cooling-down methods had a definite effect on their energy dissipation capacity; no Masing phenomenon was found in the tested structural steels. Finally, a set of skeleton curves were proposed for the four types of structural steels under cyclic loading based on the Ramberg–Osgood model, which could serve as the foundation for the seismic capacity evaluation of steel structures after a fire.
Preparation of Microstructure Laser Ablation and Multiple Acid-Etching Composites on the Surfaces of Medical Titanium Alloy TC4 by Laser Ablation and Multiple Acid-Etching, and Study of Frictional Properties of the Processed Surfaces
Authors: Chen Rifan , Xu Jiangmin , Zhao Fuhai , Wu Youchao , Zhang Jiajun
Published: July 2022

In this paper, four array patterns were first designed by observing the structural features of the surface microstructures of pig bones and tree frog paws on the titanium alloy surface bionically. Then, the optimal parameters for laser processing were determined experimentally, and the optimized processing parameters were used to prepare micron-scale bumps on the titanium alloy surface and to investigate the relationship between the weaving height and the processing times. Finally, multiple acid etching was used to prepare nanoscale holes on the surface of the titanium alloy. It was found that the multiple acid etching could not only prepare nanoscale holes on the surface of the titanium alloy, but could also well eliminate the slag left on the surface of titanium alloy by laser ablation. Based on the above study, this paper also analyzed the effect of micro-nano structure on the friction properties of the titanium alloy surface from three aspects—theory, hardness test and friction coefficient test—and experimentally analyzed the effect of single-factor and multi-factor coupling of structural features on the friction properties of the titanium alloy surface. It was found that the optimal mean friction coefficient was 0.0902, corresponding to the characteristic values of 0.66 for the shape, 200 μm for the edge length and 60 μm for the height.
Environmental Selection Shapes Bacterial Community Composition in Traditionally Fermented Maize-Based Foods from Benin, Tanzania and Zambia
Authors: de Jong Maarten , Alekseeva Anna Y. , Miraji Kulwa F. , Phiri Sydney , Linnemann Anita R. , Schoustra Sijmen E.
Published: July 2022

Natural (microbial) communities are complex ecosystems with many interactions and cross-dependencies. Among other factors, selection pressures from the environment are thought to drive the composition and functionality of microbial communities. Fermented foods, when processed using non-industrial methods, harbor such natural microbial communities. In non-alcoholic fermented foods the fermenting microbiota is commonly dominated by 4–10 species of bacteria, which make them suitable model systems to study ecosystem assembly and functioning. In this study, we assess the influence of the environment on the composition of microbial communities of traditional fermented products from Africa. We compare differences between microbial communities that are found in similar products but come from different countries, hypothesizing they experience different environmental selection pressures. We analyzed bacterial community composition in 36 samples of various cereal-based fermented foods from Benin, Tanzania and Zambia using 16S rDNA amplicon sequencing. The differential abundance analysis indicates that the bacterial communities of fermented foods from the three countries are dominated by mostly lactic acid bacteria belonging to the genera of Lactobacillus, Weisella and Curvibacter. The samples from Zambia contain the most dissimilar microbial communities in comparison with samples from Benin and Tanzania. We propose this is caused by the relatively low temperature in Zambia, suggesting that indeed environmental selection can shape community composition of fermenting microbes.
Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam
Authors: Stoakes Emily , Savva George M. , Coates Ruby , Tejera Noemi , Poolman Mark G. , Grant Andrew J. , Wain John , Singh Dipali
Published: July 2022

Campylobacter jejuni, the major cause of bacterial foodborne illness, is also a fastidious organism that requires strict growth requirements in the laboratory. Our aim was to study substrate utilisation and energy metabolism in non-growing C. jejuni to investigate the ability of these bacteria to survive so effectively in the food chain. We integrated phenotypic microarrays and genome-scale metabolic modelling (GSM) to investigate the survival of C. jejuni on 95 substrates. We further investigated the underlying metabolic re-adjustment associated with varying energy demands on each substrate. We identified amino acids, organic acids and H2, as single substrates supporting survival without growth. We identified several different mechanisms, which were used alone or in combination, for ATP production: substrate-level phosphorylation via acetate kinase, the TCA cycle, and oxidative phosphorylation via the electron transport chain that utilised alternative electron donors and acceptors. The benefit of ATP production through each of these mechanisms was associated with the cost of enzyme investment, nutrient availability and/or O2 utilisation. C. jejuni can utilise a wide range of substrates as energy sources, including organic acids commonly used for marination or preservation of ingredients, which might contribute to the success of their survival in changing environments.
Determining the International Spread of B.1.1.523 SARS-CoV-2 Lineage with a Set of Mutations Highly Associated with Reduced Immune Neutralization
Authors: Zemaitis Lukas , Alzbutas Gediminas , Gecys Dovydas , Pautienius Arnoldas , Ugenskiene Rasa , Sukys Marius , Lesauskaite Vaiva
Published: July 2022

Here, we report the emergence of the variant lineage B.1.1.523 that contains a set of mutations including 156_158del, E484K and S494P in the spike protein. E484K and S494P are known to significantly reduce SARS-CoV-2 neutralization by convalescent and vaccinated sera and are considered as mutations of concern. Lineage B.1.1.523 presumably originated in the Russian Federation and spread across European countries with the peak of transmission in April–May 2021. The B.1.1.523 lineage has now been reported from 31 countries. In this article, we analyze the possible origin of this mutation subset and its immune response using in silico methods.
Graphene in Solid-State Batteries: An Overview
Authors: Pervez Syed Atif , Madinehei Milad , Moghimian Nima
Published: July 2022

Solid-state batteries (SSBs) have emerged as a potential alternative to conventional Li-ion batteries (LIBs) since they are safer and offer higher energy density. Despite the hype, SSBs are yet to surpass their liquid counterparts in terms of electrochemical performance. This is mainly due to challenges at both the materials and cell integration levels. Various strategies have been devised to address the issue of SSBs. In this review, we have explored the role of graphene-based materials (GBM) in enhancing the electrochemical performance of SSBs. We have covered each individual component of an SSB (electrolyte, cathode, anode, and interface) and highlighted the approaches using GBMs to achieve stable and better performance. The recent literature shows that GBMs impart stability to SSBs by improving Li+ ion kinetics in the electrodes, electrolyte and at the interfaces. Furthermore, they improve the mechanical and thermal properties of the polymer and ceramic solid-state electrolytes (SSEs). Overall, the enhancements endowed by GBMs will address the challenges that are stunting the proliferation of SSBs.
Atropine-Phosphotungestate Polymeric-Based Metal Oxide Nanoparticles for Potentiometric Detection in Pharmaceutical Dosage Forms
Authors: Alterary Seham S. , El-Tohamy Maha F. , Mostafa Gamal A. E. , Alrabiah Haitham
Published: July 2022

The new research presents highly conductive polymeric membranes with a large surface area to volume ratio of metal oxide nanoparticles that were used to determine atropine sulfate (AT) in commercial dosage forms. In sensing and biosensing applications, the nanomaterials zinc oxide (ZnONPs) and magnesium oxide (MgONPs) were employed as boosting potential electroactive materials. The electroactive atropine phosphotungstate (AT-PT) was created by combining atropine sulfate and phosphotungstic acid (PTA) and mixing it with polymeric polyvinyl chloride (PVC) with the plasticizer o-nitrophenyl octyl ether (o-NPOE). The modified sensors AT-PT-ZnONPs or AT-PT-MgONPs showed excellent selectivity and sensitivity for the measurements of atropine with a linear concentration range of 6.0 × 10−8 − 1.0 × 10−3 and 8.0 × 10−8 − 1.0 × 10−3 mol L−1 with regression equations of E(mV) = (56 ± 0.5) log [AT] − 294 and E(mV) = (54 ± 0.5) log [AT] − 422 for AT-PT-NPs or AT-PT-MgONPs sensors, respectively. The AT-PT coated wire sensor, on the other hand, showed a Nernstian response at 4.0 × 10−6 − 1.0 × 10−3 mol L−1 and a regression equation E(mV) = (52.1 ± 0.2) log [AT] + 198. The methodology-recommended guidelines were used to validate the suggested modified potentiometric systems against various criteria.
Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health
Authors: González-Vega Jesús Gabriel , García-Ramos Juan Carlos , Chavez-Santoscoy Rocio Alejandra , Castillo-Quiñones Javier Emmanuel , Arellano-Garcia María Evarista , Toledano-Magaña Yanis
Published: July 2022

Nanomaterials (NMs) solve specific problems with remarkable results in several industrial and scientific areas. Among NMs, silver nanoparticles (AgNPs) have been extensively employed as drug carriers, medical diagnostics, energy harvesting devices, sensors, lubricants, and bioremediation. Notably, they have shown excellent antimicrobial, anticancer, and antiviral properties in the biomedical field. The literature analysis shows a selective cytotoxic effect on cancer cells compared to healthy cells, making its potential application in cancer treatment evident, increasing the need to study the potential risk of their use to environmental and human health. A large battery of toxicity models, both in vitro and in vivo, have been established to predict the harmful effects of incorporating AgNPs in these numerous areas or those produced due to involuntary exposure. However, these models often report contradictory results due to their lack of standardization, generating controversy and slowing the advances in nanotoxicology research, fundamentally by generalizing the biological response produced by the AgNP formulations. This review summarizes the last ten years’ reports concerning AgNPs’ toxicity in cellular respiratory system models (e.g., mono-culture models, co-cultures, 3D cultures, ex vivo and in vivo). In turn, more complex cellular models represent in a better way the physical and chemical barriers of the body; however, results should be used carefully so as not to be misleading. The main objective of this work is to highlight current models with the highest physiological relevance, identifying the opportunity areas of lung nanotoxicology and contributing to the establishment and strengthening of specific regulations regarding health and the environment.
Quantitative Deviation of Nanocrystals Using the RIR Method in X-ray Diffraction (XRD)
Authors: Huang Qinyuan , Wang Chunjian , Shan Quan
Published: July 2022

The reference intensity ratio (RIR) method, using X-ray diffraction (XRD), is considered one most of the rapid and convenient approaches for phase quantification in multi-phase mixture, in which nanocrystals are commonly contained in a mixture and cause a broadening of the diffraction peak, while another broadening factor, instrumental broadening, does not attract enough attention in related quantitative analysis. Despite the specimen consisting of 50 wt.% TiO2 nanomaterials (nano-TiO2) and 50 wt.% microscale ZnO powder, the nano-TiO2 quantitative result changes from 56.53% to 43.33% that occur as a variation of instrumental broadening are caused by divergence slit adjustment. This deviation could be accounted through a mathematical model that involves instrumental broadening. The research in this paper might provide a useful guide for developing an approach to measure accuracy quantification in unknown multi-phase mixtures
Development of Multifunctional Nanoparticles for Therapy and/or Diagnosis
Authors: Cammas-Marion Sandrine
Published: July 2022

The design of multifunctional nanoparticles for diagnostic and/or therapeutic purposes continues to be a subject of tremendous research [...]
Antimony Nanoparticles Encapsulated in Self-Supported Organic Carbon with a Polymer Network for High-Performance Lithium-Ion Batteries Anode
Authors: Wang Zhaomin , Zeng Fanming , Zhang Dongyu , Shen Yabin , Wang Shaohua , Cheng Yong , Li Chun , Wang Limin
Published: July 2022

Antimony (Sb) demonstrates ascendant reactive activation with lithium ions thanks to its distinctive puckered layer structure. Compared with graphite, Sb can reach a considerable theoretical specific capacity of 660 mAh g−1 by constituting Li3Sb safer reaction potential. Hereupon, with a self-supported organic carbon as a three-dimensional polymer network structure, Sb/carbon (3DPNS-Sb/C) composites were produced through a hydrothermal reaction channel followed by a heat disposal operation. The unique structure shows uniformitarian Sb nanoparticles wrapped in a self-supported organic carbon, alleviating the volume extension of innermost Sb alloying, and conducive to the integrality of the construction. When used as anodes for lithium-ion batteries (LIBs), 3DPNS-Sb/C exhibits a high invertible specific capacity of 511.5 mAh g−1 at a current density of 0.5 A g−1 after 100 cycles and a remarkable rate property of 289.5 mAh g−1 at a current density of 10 A g−1. As anodes, LIBs demonstrate exceptional electrochemical performance.
Applications of Nano Hydroxyapatite as Adsorbents: A Review
Authors: Balasooriya Iresha Lakmali , Chen Jia , Korale Gedara Sriyani Menike , Han Yingchao , Wickramaratne Merita Nirmali
Published: July 2022

Nano hydroxyapatite (Ca10(PO4)6(OH)2, HAp) has aroused widespread attention as a green and environmentally friendly adsorbent due to its outstanding ability in removing heavy metal ions, radio nuclides, organic pollutants and fluoride ions for wastewater treatment. The hexagonal crystal structure of HAp supports the adsorption mechanisms including ionic exchange reaction, surface complexation, the co-precipitation of new partially soluble phases and physical adsorption such as electrostatic interaction and hydrogen bonding. However, nano HAp has some drawbacks such as agglomeration and a significant pressure drop during filtration when used in powder form. Therefore, instead of using nano HAp alone, researchers have worked on modificationsand composites of nano HAp to overcome these issues and enhance the adsorption capacity. The modification of cationic doping and organic molecule grafting for nano HAp can promote the immobilization of ions and then increase adsorption capacity. Developing nano HAp composite with biopolymers such as gelatin, chitosan and chitin has proven to obtain a synergetic effect for improving the adsorption capacity of composites, in which nano HAp fixed and dispersed in polymers can playmuch more of a role for adsorption. This review summarizes the adsorption properties and adsorbent applications of nano HAp as well as the methods to enhance the adsorption capacity of nano HAp.
Protein Intake and Frailty in Older Adults: A Systematic Review and Meta-Analysis of Observational Studies
Authors: Coelho-Junior Hélio José , Calvani Riccardo , Picca Anna , Tosato Matteo , Landi Francesco , Marzetti Emanuele
Published: July 2022

Background: The present systematic review and meta-analysis investigated the cross-sectional and longitudinal associations between protein intake and frailty in older adults. Methods: We conducted a systematic review and meta-analysis of cross-sectional and longitudinal studies that investigated the association between protein intake and frailty in older adults. Cross-sectional, case-control, and longitudinal cohort studies that investigated the association between protein intake and frailty as a primary or secondary outcome in people aged 60+ years were included. Studies published in languages other than English, Italian, Portuguese, or Spanish were excluded. Studies were retrieved on 31 January 2022. Results: Twelve cross-sectional and five longitudinal studies that investigated 46,469 community-dwelling older adults were included. The meta-analysis indicated that absolute, bodyweight-adjusted, and percentage of protein relative to total energy consumption were not cross-sectionally associated with frailty. However, frail older adults consumed significantly less animal-derived protein than robust people. Finally, high protein consumption was associated with a significantly lower risk of frailty. Conclusions: Our pooled analysis indicates that protein intake, whether absolute, adjusted, or relative to total energy intake, is not significantly associated with frailty in older adults. However, we observed that frail older adults consumed significantly less animal protein than their robust counterparts.
Negative Physical Self-Concept Is Associated to Low Cardiorespiratory Fitness, Negative Lifestyle and Poor Mental Health in Chilean Schoolchildren
Authors: Delgado-Floody Pedro , Soto-García Diego , Caamaño-Navarrete Felipe , Carter-Thuillier Bastián , Guzmán-Guzmán Iris Paola
Published: July 2022

Background: Evidence suggests that physical self-concept (PSC) is linked to well-being in children and adolescents. Objective: The objective was to investigate the association of PSC with mental health (i.e., depression and body image), physical status (i.e., fitness and weight status) and lifestyle (physical activity (PA) patterns and nutritional level) in Chilean schoolchildren. Methods: A total of 617 schoolchildren (n = 271 girls and n = 346 boys) aged 10–14 years participated in this study. Self-concept, depression and body image dissatisfaction were determined by questionnaires. Physical fitness, PA, screen time (ST), Mediterranean diet (MD) adherence and anthropometric parameters were also included. Results: Poor PSC was linked to bad cardiorespiratory fitness (CRF) (<42 VO2max) (OR 1.64; 95%CI 1.12–2.34; p = 0.01), severe body image dissatisfaction (OR 2.51, 95%CI 0.99–6.35; p = 0.05), ST of more than two hours a day (OR 2.1; 95%CI 1.41–3.12; p < 0.001), PA after school of no more than two hours per week (OR 1.52; 95%CI 1.08–2.13; p = 0.015) and depression (OR 1.80; 95%CI 1.1–2.92; p = 0.017). High nutritional level showed an association with general PSC and general self-concept (p < 0.05). Absence of body image dissatisfaction was related to general self-concept (p < 0.01) and physical condition dimensions (p < 0.05). Conclusions: PSC is associated with CRF, PA after school, ST and nutritional level. According to mental health variables, poor PSC is related to depression in Chilean schoolchildren. Therefore, promoting a healthy lifestyle among children should be a target of community- and school-based interventions to promote PSC.
Rural Household Food Insecurity among Latino Immigrants during the COVID-19 Pandemic
Authors: Payán Denise Diaz , Perez-Lua Fabiola , Goldman-Mellor Sidra , Young Maria-Elena De Trinidad
Published: July 2022

U.S. food insecurity rates rapidly increased during the COVID-19 pandemic, with disproportionate impacts on Latino immigrant households. We conducted a qualitative study to investigate how household food environments of rural Latino immigrants were affected during the COVID-19 pandemic. Thirty-one respondents (42% from low food security households) completed interviews (July 2020–April 2021) across four rural counties in California. A rural household food security conceptual framework was used to analyze the data. Early in the pandemic, food availability was impacted by school closures and the increased consumption of meals/snacks at home; food access was impacted by reduced incomes. Barriers to access included limited transportation, excess distance, and lack of convenience. Key resources for mitigating food insecurity were the Supplemental Nutrition Assistance Program (SNAP), the Pandemic Electronic Benefits Transfer (P-EBT), school meals, charitable food programs, and social capital, although the adequacy and acceptability of charitable food distributions were noted issues. Respondents expressed concern about legal status, stigma, and the public charge rule when discussing barriers to government nutrition assistance programs. They reported that food pantries and P-EBT had fewer access barriers. Positive coping strategies included health-promoting food substitutions and the reduced consumption of meals outside the home. Results can inform the development of policy and systems interventions to decrease food insecurity and nutrition-related health disparities among rural Latino immigrants.
Healthy Taiwanese Eating Approach (TEA) toward Total Wellbeing and Healthy Longevity
Authors: Pan Wen-Harn , Wu Szu-Yun , Yeh Nai-Hua , Hung Shu-Yi
Published: July 2022

A healthy dietary pattern review for Asian countries is scarce, which is crucial for guiding healthy eating. We reviewed Taiwanese dietary pattern discovery studies. Included were 19 studies, the majority of which employed dimension reduction methods to find dietary patterns associated with various health conditions. To show what is a high or low intake of foods in Taiwan, we also report the average dietary content and the 25th and 75th percentile values of the adult population for six food groups gathered by the Nutrition and Health Survey in Taiwan, 2017–2020. The healthy Taiwanese dietary approach is cohesive across multiple health outcomes occurring at different ages. It is featured with higher intakes of plant-based foods, aquatic foods, and some beneficial ethnic foods (soy products), drinks (tea), and cooking methods (boiling and steaming); lower intakes of fast foods, fatty and processed meats, sugar, salt rich foods/drinks, and fried foods; but with mixed findings for dairy and egg. Yet, the average Taiwanese person consumed many refined staple foods and livestock, but not sufficient vegetables, fruits, whole grains and roots, beans, and nuts. Dairy consumption remains low. In conclusion, Taiwanese discovery studies point to a mortality-lowering total wellbeing dietary pattern consistent with the current knowledge, which discloses potential benefits of soy product, tea, and boiling and steaming.
Temporal Dynamics of the Intestinal Microbiome Following Short-Term Dietary Restriction
Authors: Anderson Erik M. , Rozowsky Jared M. , Fazzone Brian J. , Schmidt Emilie A. , Stevens Bruce R. , O’Malley Kerri A. , Scali Salvatore T. , Berceli Scott A.
Published: July 2022

Short-term dietary restriction has been proposed as an intriguing pre-operative conditioning strategy designed to attenuate the surgical stress response and improve outcomes. However, it is unclear how this nutritional intervention influences the microbiome, which is known to modulate the systemic condition. Healthy individuals were recruited to participate in a four-day, 70% protein-restricted, 30% calorie-restricted diet, and stool samples were collected at baseline, after the restricted diet, and after resuming normal food intake. Taxonomy and functional pathway analysis was performed via shotgun metagenomic sequencing, prevalence filtering, and differential abundance analysis. High prevalence species were altered by the dietary intervention but quickly returned to baseline after restarting a regular diet. Composition and functional changes after the restricted diet included the decreased relative abundance of commensal bacteria and a catabolic phenotype. Notable species changes included Faecalibacterium prausnitzii and Roseburia intestinalis, which are major butyrate producers within the colon and are characteristically decreased in many disease states. The macronutrient components of the diet might have influenced these changes. We conclude that short-term dietary restriction modulates the ecology of the gut microbiome, with this modulation being characterized by a relative dysbiosis.
ER-Stress and Senescence Coordinately Promote Endothelial Barrier Dysfunction in Diabetes-Induced Atherosclerosis
Authors: Fatima Sameen , Ambreen Saira , Mathew Akash , Elwakiel Ahmed , Gupta Anubhuti , Singh Kunal , Krishnan Shruthi , Rana Rajiv , Khawaja Hamzah , Gupta Dheerendra , Manoharan Jayakumar , Besler Christian , Laufs Ulrich , Kohli Shrey , Isermann Berend , Shahzad Khurrum
Published: July 2022

Diabetes mellitus is hallmarked by accelerated atherosclerosis, a major cause of mortality among patients with diabetes. Efficient therapies for diabetes-associated atherosclerosis are absent. Accelerated atherosclerosis in diabetic patients is associated with reduced endothelial thrombomodulin (TM) expression and impaired activated protein C (aPC) generation. Here, we directly compared the effects of high glucose and oxidized LDL, revealing that high glucose induced more pronounced responses in regard to maladaptive unfolded protein response (UPR), senescence, and vascular endothelial cell barrier disruption. Ex vivo, diabetic ApoE−/− mice displayed increased levels of senescence and UPR markers within atherosclerotic lesions compared with nondiabetic ApoE−/− mice. Activated protein C pretreatment maintained barrier permeability and prevented glucose-induced expression of senescence and UPR markers in vitro. These data suggest that high glucose-induced maladaptive UPR and associated senescence promote vascular endothelial cell dysfunction, which—however—can be reversed by aPC. Taken together, current data suggest that reversal of glucose-induced vascular endothelial cell dysfunction is feasible.
Metagenomic Changes of Gut Microbiota following Treatment of Helicobacter pylori Infection with a Simplified Low-Dose Quadruple Therapy with Bismuth or Lactobacillus reuteri
Authors: Dore Maria Pina , Sau Rosangela , Niolu Caterina , Abbondio Marcello , Tanca Alessandro , Bibbò Stefano , Loria Mariafrancesca , Pes Giovanni Mario , Uzzau Sergio
Published: July 2022

Background: Probiotic supplementation to antibiotic regimens against Helicobacter pylori infection has been proposed to improve eradication rate and to decrease detrimental effects on gut microbiota. Aims: To evaluate microbiota modifications due to a low-dose quadruple therapy with bismuth or Lactobacillus reuteri. Methods: Forty-six patients infected with H. pylori were prospectively enrolled in a single-centre, randomized controlled trial to receive b.i.d. with meals for 10 days low-dose quadruple therapy consisting of rabeprazole 20 mg and bismuth (two capsules of Pylera® plus 250 mg each of tetracycline and metronidazole), or the same dose of rabeprazole and antibiotics plus Gastrus® (L. reuteri), one tablet twice-a-day for 27 days. Stool samples were collected at the enrolment, at the end and 30–40 days after the treatment. Gut microbiota composition was investigated with 16S rRNA gene sequencing. Results: Eradication rate was by ITT 78% in both groups, and by PP analysis 85.7% and 95.5% for Gastrus® and bismuth group, respectively. Alpha and beta diversity decreased at the end of treatment and was associated with a reduction of bacterial genera beneficial for gut homeostasis, which was rescued 30–40 days later in both groups, suggesting a similar impact of the two regimens in challenging bacterial community complexity. Conclusions: Low-dose bismuth quadruple therapy proved to be effective with lower costs and amount of antibiotics and bismuth. Gastrus® might be an option for patients with contraindications to bismuth. L. reuteri was unable to significantly counteract dysbiosis induced by antibiotics. How to administer probiotics to prevent gut microbiota alterations remains an open question.
Whale-Associated Microbial Communities Remain Remarkably Stable despite Massive Water Community Disruption in a Managed Artificial Marine Environment
Authors: Van Bonn William , Oliaro Francis , Pinnell Lee
Published: July 2022

Highly managed and built environments such as zoos and aquaria provide a rich source of standardized environmental monitoring data over periods of years to decades. A fifty percent water change in an 11.4-million-liter indoor artificial sea water system housing three species of marine mammals was conducted over a two-month period. Using 16S rRNA gene sequencing, the microbial community structure of the system water and three host sites (feces, skin, and exhaled breath “chuff”) of whales housed in the system were characterized. Diversity measures confirmed massive disruption to the water community structure as an expected result of the water change. Host site-associated communities remained remarkably stable. Improved understanding of host microbial community dynamics in response to environmental system perturbations allows for sound management decisions toward optimizing conditions for resident animals.
Schistosome Sulfotransferases: Mode of Action, Expression and Localization
Authors: Guzman Meghan A. , Rugel Anastasia , Alwan Sevan N. , Tarpley Reid , Taylor Alexander B. , Chevalier Frédéric D. , Wendt George R. , Collins James J. , Anderson Timothy J. C. , McHardy Stanton F. , LoVerde Philip T.
Published: July 2022

Oxamniquine (OXA) is a prodrug activated by a sulfotransferase (SULT) that was only active against Schistosoma mansoni. We have reengineered OXA to be effective against S. haematobium and S. japonicum. Three derivatives stand out, CIDD-0066790, CIDD-0072229, and CIDD-0149830 as they kill all three major human schistosome species. However, questions remain. Is the OXA mode of action conserved in derivatives? RNA-interference experiments demonstrate that knockdown of the SmSULT, ShSULT, and SjSULT results in resistance to CIDD-0066790. Confirming that the OXA-derivative mode of action is conserved. Next is the level of expression of the schistosome SULTs in each species, as well as changes in SULT expression throughout development in S. mansoni. Using multiple tools, our data show that SmSULT has higher expression compared to ShSULT and SjSULT. Third, is the localization of SULT in the adult, multicellular eucaryotic schistosome species. We utilized fluorescence in situ hybridization and uptake of radiolabeled OXA to determine that multiple cell types throughout the adult schistosome worm express SULT. Thus, we hypothesize the ability of many cells to express the sulfotransferase accounts for the ability of the OXA derivatives to kill adult worms. Our studies demonstrate that the OXA derivatives are able to kill all three human schistosome species and thus will be a useful complement to PZQ.
“Endothelial Antibody Factory” at the Blood Brain Barrier: Novel Approach to Therapy of Neurodegenerative Diseases
Authors: Thinard Reynald , Farkas Attila E. , Halasa Marta , Chevalier Melanie , Brodaczewska Klaudia , Majewska Aleksandra , Zdanowski Robert , Paprocka Maria , Rossowska Joanna , Duc Lam Tri , Greferath Ruth , Krizbai Istvan , Van Leuven Fred , Kieda Claudine , Nicolau Claude
Published: July 2022

The failures of anti-β-amyloid immunotherapies suggested that the very low fraction of injected antibodies reaching the brain parenchyma due to the filtering effect of the BBB may be a reason for the lack of therapeutic effect. However, there is no treatment, as yet, for the amyotrophic lateral sclerosis (ALS) despite substantial evidence existing of the involvement of TDP-43 protein in the evolution of ALS. To circumvent this filtering effect, we have developed a novel approach to facilitate the penetration of antibody fragments (Fabs) into the brain parenchyma. Leveraging the homing properties of endothelial progenitor cells (EPCs), we transfected, ex vivo, such cells with vectors encoding anti-β-amyloid and anti-TDP43 Fabs turning them into an “antibody fragment factory”. When injected these cells integrate into the BBB, where they secrete anti-TDP43 Fabs. The results showed the formation of tight junctions between the injected engineered EPCs and the unlabeled resident endothelial cells. When the EPCs were further modified to express the anti-TDP43 Fab, we could observe integration of these cells into the vasculature and the secretion of Fabs. Results confirm that production and secretion of Fabs at the BBB level leads to their migration to the brain parenchyma where they might exert a therapeutic effect.
Efficacy of Vafidemstat in Experimental Autoimmune Encephalomyelitis Highlights the KDM1A/RCOR1/HDAC Epigenetic Axis in Multiple Sclerosis
Authors: Cavalcanti Fernando , Gonzalez-Rey Elena , Delgado Mario , Falo Clara P. , Mestre Leyre , Guaza Carmen , O’Valle Francisco , Lufino Michele M. P. , Xaus Jordi , Mascaró Cristina , Lunardi Serena , Sacilotto Natalia , Dessanti Paola , Rotllant David , Navarro Xavier , Herrando-Grabulosa Mireia , Buesa Carlos , Maes Tamara
Published: July 2022

Lysine specific demethylase 1 (LSD1; also known as KDM1A), is an epigenetic modulator that modifies the histone methylation status. KDM1A forms a part of protein complexes that regulate the expression of genes involved in the onset and progression of diseases such as cancer, central nervous system (CNS) disorders, viral infections, and others. Vafidemstat (ORY-2001) is a clinical stage inhibitor of KDM1A in development for the treatment of neurodegenerative and psychiatric diseases. However, the role of ORY-2001 targeting KDM1A in neuroinflammation remains to be explored. Here, we investigated the effect of ORY-2001 on immune-mediated and virus-induced encephalomyelitis, two experimental models of multiple sclerosis and neuronal damage. Oral administration of ORY-2001 ameliorated clinical signs, reduced lymphocyte egress and infiltration of immune cells into the spinal cord, and prevented demyelination. Interestingly, ORY-2001 was more effective and/or faster acting than a sphingosine 1-phosphate receptor antagonist in the effector phase of the disease and reduced the inflammatory gene expression signature characteristic ofEAE in the CNS of mice more potently. In addition, ORY-2001 induced gene expression changes concordant with a potential neuroprotective function in the brain and spinal cord and reduced neuronal glutamate excitotoxicity-derived damage in explants. These results pointed to ORY-2001 as a promising CNS epigenetic drug able to target neuroinflammatory and neurodegenerative diseases and provided preclinical support for the subsequent design of early-stage clinical trials.
Chemical and Structural Elucidation of Lignin and Cellulose Isolated Using DES from Bagasse Based on Alkaline and Hydrothermal Pretreatment
Authors: Wang Na , Xu Baoming , Wang Xinhui , Lang Jinyan , Zhang Heng
Published: July 2022

The separation of cellulose, hemicellulose, and lignin components using deep eutectic solvent, which is a green solvent, to obtain corresponding chemicals can realize the effective separation and high-value utilization of these components at low cost. In this study, we used waste biomass sugarcane bagasse as the raw material, choline chloride as the hydrogen bond acceptor, and lactic acid as the hydrogen bond donor to synthesize a deep eutectic solvent of choline chloride/lactic acid (L-DES) and treated sugarcane bagasse pretreated by alkali or hydrothermal methods to separate cellulose, hemicellulose, and lignin. In addition, we comparatively studied the effect of different pretreatment methods on lignin removal by DES and found that the lignin removal rate by L-DES after alkaline pretreatment was significantly higher than that after hydrothermal pretreatment, and the mechanism of action causing this difference is discussed.
Biocentrismo Igualitario
Authors: Mendieta Toledo Lenin Byron
Published: July 2022

La filosofía siempre está cuestionando lo establecido, siempre le encuentra la pregunta a la respuesta, es por ello por lo que Gadamer manifiesta que el discurso es una interpretación de una interpretación infinita y siempre existirá el ¿Por qué?. El objetivo del ensayo fue establecer nuestra postura frente al Biocentrismo igualitario. Realizamos algunas revisiones documentales y establecimos un diálogo y crítica de los planteamientos. Concluimos que el Biocentrismo es una teoría eurocéntrica que se practica en nuestra filosofía indígena desde antes de la colonia, nuestra postura es que el defender la biodiversidad es una obligación y una necesidad del hombre como un gesto de gratitud para con la naturaleza, que la filosofía del Sumak Kawsay es aquella que establece el cuidado que debe realizar el hombre con nuestra Pachamama.