bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Hop Freshness on Kettle-Hopped Beers
Authors: Rutnik Ksenija , Ocvirk Miha , Košir Iztok Jože
Published: December 2023

Hops are an indispensable ingredient in beer, and the differences in their chemical composition impart the various tastes and aromas associated with different beers. However, during storage, hops undergo changes in their chemical composition. Here, the changes in aroma and bitterness of kettle-hopped beers were evaluated in an experiment conducted on three different hop varieties (Aurora, Celeia and Styrian Wolf) with five different hop storage index (HSI) values (0.3–0.7). Hops were added to boiling wort for 5, 45 and 90 min. Alpha-acids, iso-alpha-acids, humulinones, bitterness units and hop aroma compounds in the samples were chemically analysed. All samples also underwent sensorial analysis. The old hops were not problematic in terms of bitterness or early hopping time. However, later additions of old hops reduced the quality and intensity of the hop aroma. The limit value for use without negative consequences for kettle hopping was set at HSI 0.5 for Aurora and Celeia and HSI 0.6 for Styrian Wolf.
Characteristic Profile of the Hazardous, Nutritional, and Taste-Contributing Compounds during the Growth of Argopecten irradians with Different Shell Colors
Authors: Wang Teng , Peng Jixing , Zhao Xinnan , Lin Yichen , Song Dongru , Zhao Yanfang , Jiang Yanhua , Wu Haiyan , Geng Qianqian , Zheng Guanchao , Guo Mengmeng , Tan Zhijun
Published: December 2023

Bay scallops (Argopecten irradians; A. irradians) are shellfish with high nutritional and economic value. However, nutritional studies on A. irradians with different shell colors are limited. This study examines the hazardous, nutritional, and taste-contributing compounds during the growth of A. irradians with different shell colors. During the growth of A. irradians, the hazardous contents were below the standard limit. Changes in the nutritional and taste-contributing compounds between months were more significant than shell color. Bay scallops had more fats, total fatty acids, and taste-contributing compounds in August and more proteins, essential fatty acids, vitamin D, vitamin B12, Cu, and Zn in September and October. In October, the golden shell color strain had more proteins, essential fatty acids, vitamin D, vitamin B12, Cu, and Zn, while the purple shell color strain had more taste-contributing compounds. A. irradians had better taste in August and higher nutritional value in September and October. In October, the golden shell color strain has higher nutritional value, and the purple shell color strain has better commercial value and taste. The correlation analysis indicates that the nutritional quality of bay scallops is affected by age (months), shell color, and seawater environment.
Consumer Expectation and Perception of Farmed Rainbow Trout (Oncorhynchus mykiss) Fed with Insect Meal (Tenebrio molitor)
Authors: Magnani Martina , Claret Anna , Gisbert Enric , Guerrero Luis
Published: December 2023

In recent years, insect meal has attracted increasing interest as an innovative protein source to replace fish meal in feed formulations due to its valuable nutritional profile. This research aimed to compare the effects of different levels of dietary inclusion of the yellow mealworm beetle (T. molitor) larvae meal on the sensory quality of rainbow trout (Oncorhynchus mykiss) fillets and retrospectively on the acceptability of this protein source to consumers. The results showed that the inclusion of T. molitor larvae meal did not induce sensory changes in the trout fillets, while regarding consumer acceptability and willingness to buy and pay, it was shown that a certain level of rejection towards this alternative protein still exists. The work described in this scientific manuscript adds more knowledge on the study of consumer acceptability of this protein source.
A Study of a New Certified Reference Material for Accurate Determination of the Main Fusarium Mycotoxins in Whole-Wheat Flour
Authors: Li Li , Li Peng , Wu Yu , Ye Jin , Li Zongwang , Wang Songxue
Published: December 2023

Matrix certified reference materials (CRMs) play a critical role in analytical method validation and the assurance of reliable measurement results. A certified reference material (GBW(E)100813) for whole-wheat flour was developed to ensure an accurate and reliable measurement of the main Fusarium mycotoxins (deoxynivalenol (DON), nivalenol (NIV), deoxynivalenol-3-glucoside (DON-3G), and zearalenone (ZEN)). CRM candidates were prepared using sun-drying, grinding, sieving, homogenising, packaging, and gamma irradiation. The final produced CRM was packaged at 50 g per unit and stored at 20 °C. Certification was performed using isotope dilution-liquid chromatography–tandem mass spectrometry. CRM characterization was performed in eight laboratories in accordance with the requirements of ISO Guide 35. The certified values and expanded uncertainties (at a confidence of 95%, k = 2) for DON, NIV, DON-3G, and ZEN were determined to be 0.98 ± 0.12 mg/kg, 1.37 ± 0.20 mg/kg, 242 ± 35 μg/g, and 382 ± 50 μg/g. The CRM was sufficiently homogeneous between and within bottles, and remained stable for up to 12 months at 20 °C and 9 days below 40 °C for transportation. Thus, CRM can be used for quality control and method validation to ensure the accurate and reliable quantification of the main Fusarium mycotoxins in whole-wheat flour.
Antioxidant Capacity of Free and Peptide Tryptophan Residues Determined by the ORAC (Oxygen Radical Absorbance Capacity) Assay Is Modulated by Radical-Radical Reactions and Oxidation Products
Authors: Figueroa Juan David , Barroso-Torres Noreima , Morales Marcela , Herrera Bárbara , Aranda Mario , Dorta Eva , López-Alarcón Camilo
Published: December 2023

The ORAC (Oxygen Radical Absorbance Capacity) assay is commonly employed for determining the antioxidant capacity of bioactive peptides. To gain insights into the meaning of this index for peptides containing a single Trp, we studied the consumption of this residue and fluorescein (FLH, the probe of ORAC method), induced by radicals generated by AAPH (2,2′-Azo-bis(2-amidinopropane) dihydrochloride) thermolysis. ORAC values were rationalized from kinetics and computational calculations of bond dissociation energies (BDE) of the N-H bond (indole ring of Trp). Free Trp, di- and tri- peptides, and three larger peptides were studied. Solutions containing 70 nM FLH, 1–5 μM free Trp or peptides, and 10 mM AAPH were incubated at 37 °C in phosphate buffer. Kinetic studies showed that FLH minimally affected Trp consumption. However, a clear protection of FLH, characterized by pseudo-lag times, was evidenced, reflecting radical-radical reactions and FLH repairing. Peptides showed similar ORAC values (~1.9–2.8 Trolox equivalents), while BDE varied between 91.9 and 103.5 kcal. These results, added to the protection of FLH observed after total consumption of Trp, indicate a lack of discrimination of the assay for the chemical structure of peptides and the contribution of oxidation products to the index.
Maclaurin-Type Integral Inequalities for GA-Convex Functions Involving Confluent Hypergeometric Function via Hadamard Fractional Integrals
Authors: Chiheb Tarek , Meftah Badreddine , Moumen Abdelkader , Bouye Mohamed
Published: December 2023

In this manuscript, by using a new identity, we establish some new Maclaurin-type inequalities for functions whose modulus of the first derivatives are GA-convex functions via Hadamard fractional integrals.
Assessing Tele-Oral Medicine in Saudi Arabia: A Cross-Sectional Study on Specialists’ Experiences and Effectiveness in Oral Healthcare
Authors: Akeel Sara , Almazrooa Soulafa , Ali Sarah Alfarabi , Alhindi Nada A. , Alhamed Sana , Felemban Osama M. , Mansour Ghada , Sabbahi Dania , Binmadi Nada , Mawardi Hani
Published: December 2023

Introduction: Teledentistry is an emerging tool to exchange medical information and clinical images to facilitate the diagnosis, prevention, and treatment of oral diseases and patient assurance and education. Considering the shortage of oral medicine specialists in Saudi Arabia, this study aims to assess the experiences of dental specialists with tele-oral medicine and its potential applicability in addressing this shortage. Materials and methods: This was a pilot, cross-sectional study conducted among specialists in the field of oral medicine from January 2020 to March 2020. A total of 16 preselected cases with oral lesions, including clinical history and images, were developed, validated, and shared via email with study participants. Each case included questions on differential diagnosis, provisional diagnosis, and management. The responses were recorded, analyzed, and presented as means and percentages. Results: A total of 49 subjects participated in this study and more than half were under 40 years of age and two-thirds were women. A total of 23 participants had prior experience with tele-oral medicine, mainly via WhatsApp (95.7%), and these cases were received from patients, their families, friends, or other dentists. For all study cases, the correct diagnosis score ranged between 73.50 and 100%, and correct management ranged between 51 and 98%. Conclusions: Tele-oral medicine is an effective tool that may play an important role in patient management in rural regions with a shortage of oral medicine services. Further studies with larger sample sizes and in collaboration with international centers are warranted to confirm these findings.
Improving Health Literacy of Domestic Household Disinfection Use: Readability of Consumer-Facing Information on Domestic Household Disinfectant Products on Sale in the UK—An Infodemiology Study
Authors: Moore John E. , Millar Beverley C.
Published: December 2023

Disinfectants purchased from retail outlets form the cornerstone of infection control and prevention within the domestic household. The growing utilisation of the concept of “hospital-at-home” places greater emphasis on domestic disinfection by the householder in helping to prevent the acquisition of infections within the home. No reports or data exist that indicate how readable the information provided on disinfectants is, which would help householders use disinfectants optimally. The aim of this study was therefore to quantitatively examine the readability (Flesch Reading Ease; Flesch–Kinkaid Grade Level; text metrics) of consumer (public)-facing information (n = 108) of domestic household disinfectants sourced from (i) UK high street supermarket chains (n = 4) and (ii) disinfectant manufacturers (n = 6). The readability of all supermarket and manufacturer information (n = 108) gave a mean Flesch Reading Ease score of 51.7 (target ≥ 60) and a Flesch–Kinkaid Grade Level score of 8.1 (target ≤ 8), thereby failing to achieve readability reference target values. Authors preparing information on the domestic use of disinfectants should be aware of the value of quantitative readability metrics and online tools to help support their writing of such information in order to produce materials which are within target readability values, thereby further supporting health literacy in this population and disinfectant efficacy.
Proteomic Analysis of Fractionated Eimeria tenella Sporulated Oocysts Reveals Involvement in Oocyst Wall Formation
Authors: Jia Liushu , Zhao Qiping , Zhu Shunhai , Han Hongyu , Zhao Huanzhi , Yu Yu , Yang Jia , Dong Hui
Published: December 2023

Eimeria tenella is the most pathogenic intracellular protozoan parasite of the Eimeria species. Eimeria oocyst wall biogenesis appears to play a central role in oocyst transmission. Proteome profiling offers insights into the mechanisms governing the molecular basis of oocyst wall formation and identifies targets for blocking parasite transmission. Tandem mass tags (TMT)-labeled quantitative proteomics was used to analyze the oocyst wall and sporocysts of E. tenella. A combined total of 2865 E. tenella proteins were identified in the oocyst wall and sporocyst fractions; among these, 401 DEPs were identified, of which 211 were upregulated and 190 were downregulated. The 211 up-regulated DEPs were involved in various biological processes, including DNA replication, fatty acid metabolism and biosynthesis, glutathione metabolism, and propanoate metabolism. Among these proteins, several are of interest for their likely role in oocyst wall formation, including two tyrosine-rich gametocyte proteins (EtGAM56, EtSWP1) and two cysteine-rich proteins (EtOWP2, EtOWP6). Concurrently, 96 uncharacterized proteins may also participate in oocyst wall formation. The present study significantly expands our knowledge of the proteome of the oocyst wall of E. tenella, thereby providing a theoretical basis for further understanding of the biosynthesis and resilience of the E. tenella oocyst wall.
Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe2O4 Nanoparticles
Authors: Rincon-Granados Karen L. , Vázquez-Olmos América R. , Rodríguez-Hernández Adriana-Patricia , Prado-Prone Gina , Rivera Margarita , Garibay-Febles Vicente , Almanza-Arjona Yara C. , Sato-Berrú Roberto Y. , Mata-Zamora Esther , Silva-Bermúdez Phaedra S. , Vega-Jiménez Alejandro
Published: December 2023

This study presents an in vitro analysis of the bactericidal and cytotoxic properties of hybrid films containing nickel oxide (NiO) and nickel ferrite (NiFe2O4) nanoparticles embedded in polypropylene (PP). The solvent casting method was used to synthesize films of PP, PP@NiO, and PP@NiFe2O4, which were characterized by different spectroscopic and microscopic techniques. The X-ray diffraction (XRD) patterns confirmed that the small crystallite sizes of NiO and NiFe2O4 NPs were maintained even after they were incorporated into the PP matrix. From the Raman scattering spectroscopy data, it was evident that there was a significant interaction between the NPs and the PP matrix. Additionally, the Scanning Electron Microscopy (SEM) analysis revealed a homogeneous dispersion of NiO and NiFe2O4 NPs throughout the PP matrix. The incorporation of the NPs was observed to alter the surface roughness of the films; this behavior was studied by atomic force microscopy (AFM). The antibacterial properties of all films were evaluated against Pseudomonas aeruginosa (ATCC®: 43636™) and Staphylococcus aureus (ATCC®: 23235™), two opportunistic and nosocomial pathogens. The PP@NiO and PP@ NiFe2O4 films showed over 90% bacterial growth inhibition for both strains. Additionally, the effects of the films on human skin cells, such as epidermal keratinocytes and dermal fibroblasts, were evaluated for cytotoxicity. The PP, PP@NiO, and PP@NiFe2O4 films were nontoxic to human keratinocytes. Furthermore, compared to the PP film, improved biocompatibility of the PP@NiFe2O4 film with human fibroblasts was observed. The methodology utilized in this study allows for the production of hybrid films that can inhibit the growth of Gram-positive bacteria, such as S. aureus, and Gram-negative bacteria, such as P. aeruginosa. These films have potential as coating materials to prevent bacterial proliferation on surfaces.
Toward Transgene-Free Transposon-Mediated Biological Mutagenesis for Plant Breeding
Authors: Kirov Ilya
Published: December 2023

Genetic diversity is a key factor for plant breeding. The birth of novel genic and genomic variants is also crucial for plant adaptation in nature. Therefore, the genomes of almost all living organisms possess natural mutagenic mechanisms. Transposable elements (TEs) are a major mutagenic force driving genetic diversity in wild plants and modern crops. The relatively rare TE transposition activity during the thousand-year crop domestication process has led to the phenotypic diversity of many cultivated species. The utilization of TE mutagenesis by artificial and transient acceleration of their activity in a controlled mode is an attractive foundation for a novel type of mutagenesis called TE-mediated biological mutagenesis. Here, I focus on TEs as mutagenic sources for plant breeding and discuss existing and emerging transgene-free approaches for TE activation in plants. Furthermore, I also review the non-randomness of TE insertions in a plant genome and the molecular and epigenetic factors involved in shaping TE insertion preferences. Additionally, I discuss the molecular mechanisms that prevent TE transpositions in germline plant cells (e.g., meiocytes, pollen, egg and embryo cells, and shoot apical meristem), thereby reducing the chances of TE insertion inheritance. Knowledge of these mechanisms can expand the TE activation toolbox using novel gene targeting approaches. Finally, the challenges and future perspectives of plant populations with induced novel TE insertions (iTE plant collections) are discussed.
Second Wave, Late-Stage Neuroinflammation in Cleared Brains of Aged 5xFAD Alzheimer’s Mice Detected by Macrolaser Light Sheet Microscopy Imaging
Authors: Lee Suk Hyun , Son Hye Joo
Published: December 2023

This study leverages the innovative imaging capabilities of macrolaser light-sheet microscopy to elucidate the 3D spatial visualization of AD-associated neuropathologic networks in the transparent brains of 44-week-old 5xFAD mice. Brain samples from ten AD and seven control mice were prepared through a hydrophilic tissue-clearing pipeline and immunostained with thioflavin S (β-amyloid), anti-CD11b antibody (microglia), and anti-ACSA-2 antibody (astrocytes). The 5xFAD group exhibited significantly higher average total surface volumes of β-amyloid accumulation than the control group (AD, 898,634,368 µm3 [383,355,488–1,324,986,752]; control, 33,320,178 µm3 [11,156,785–65,390,988], p = 0.0006). Within the AD group, there was significant interindividual and interindividual variability concerning the number and surface volume of individual amyloid particles throughout the entire brain. In the context of neuroinflammation, the 5xFAD group showed significantly higher average total surface volumes of anti-ACSA-2-labeled astrocytes (AD, 59,064,360 µm3 [27,815,500–222,619,280]; control, 20,272,722 µm3 [9,317,288–27,223,352], p = 0.0047) and anti-CD11b labeled microglia (AD, 51,210,100 µm3 [15,309,118–135,532,144]; control, 23,461,593 µm3 [14,499,170–27,924,110], p = 0.0162) than the control group. Contrary to the long-standing finding that early-stage neuroinflammation precedes the subsequent later-stage of neurodegeneration, our data reveal that the second wave, late-stage active neuroinflammation persists in the aged AD brains, even as they continue to show signs of ongoing neurodegeneration and significant amyloid accumulation.
Increased Lipogenesis Is Important for Hexavalent Chromium-Transformed Lung Cells and Xenograft Tumor Growth
Authors: Wise James T. F. , Kondo Kazuya
Published: December 2023

Hexavalent chromium, Cr(VI), is a known carcinogen and environmental health concern. It has been established that reactive oxygen species, genomic instability, and DNA damage repair deficiency are important contributors to the Cr(VI)-induced carcinogenesis mechanism. However, some hallmarks of cancer remain under-researched regarding the mechanism behind Cr(VI)-induced carcinogenesis. Increased lipogenesis is important to carcinogenesis and tumorigenesis in multiple types of cancers, yet the role increased lipogenesis has in Cr(VI) carcinogenesis is unclear. We report here that Cr(VI)-induced transformation of three human lung cell lines (BEAS-2B, BEP2D, and WTHBF-6) resulted in increased lipogenesis (palmitic acid levels), and Cr(VI)-transformed cells had an increased expression of key lipogenesis proteins (ATP citrate lyase [ACLY], acetyl-CoA carboxylase [ACC1], and fatty acid synthase [FASN]). We also determined that the Cr(VI)-transformed cells did not exhibit an increase in fatty acid oxidation or lipid droplets compared to their passage-matched control cells. Additionally, we observed increases in ACLY, ACC1, and FASN in lung tumor tissue compared with normal-adjacent lung tissue (in chromate workers that died of chromate-induced tumors). Next, using a known FASN inhibitor (C75), we treated Cr(VI)-transformed BEAS-2B with this inhibitor and measured cell growth, FASN protein expression, and growth in soft agar. We observed that FASN inhibition results in a decreased protein expression, decreased cell growth, and the inhibition of colony growth in soft agar. Next, using shRNA to knock down the FASN protein in Cr(VI)-transformed BEAS-2B cells, we saw a decrease in FASN protein expression and a loss of the xenograft tumor development of Cr(VI)-transformed BEAS-2B cells. These results demonstrate that FASN is important for Cr(VI)-transformed cell growth and cancer properties. In conclusion, these data show that Cr(VI)-transformation in vitro caused an increase in lipogenesis, and that this increase is vital for Cr(VI)-transformed cells.
Exogenous Serotonin (5-HT) Promotes Mesocotyl and Coleoptile Elongation in Maize Seedlings under Deep-Seeding Stress through Enhancing Auxin Accumulation and Inhibiting Lignin Formation
Authors: Zhao Xiaoqiang , Li Jiayao , Niu Yining , Hossain Zakir , Gao Xiquan , Bai Xiaodong , Mao Taotao , Qi Guoxiang , He Fuqiang
Published: December 2023

Serotonin (5-HT), an indoleamine compound, has been known to mediate many physiological responses of plants under environmental stress. The deep-seeding (≥20 cm) of maize seeds is an important cultivation strategy to ensure seedling emergence and survival under drought stress. However, the role of 5-HT in maize deep-seeding tolerance remains unexplored. Understanding the mechanisms and evaluating the optimal concentration of 5-HT in alleviating deep-seeding stress could benefit maize production. In this study, two maize inbred lines were treated with or without 5-HT at both sowing depths of 20 cm and 3 cm, respectively. The effects of different concentrations of 5-HT on the growth phenotypes, physiological metabolism, and gene expression of two maize inbred lines were examined at the sowing depths of 20 cm and 3 cm. Compared to the normal seedling depth of 3 cm, the elongation of the mesocotyl (average elongation 3.70 cm) and coleoptile (average elongation 0.58 cm), secretion of indole-3-acetic acid (IAA; average increased 3.73 and 0.63 ng g−1 FW), and hydrogen peroxide (H2O2; average increased 1.95 and 0.63 μM g−1 FW) in the mesocotyl and coleoptile were increased under 20 cm stress, with a concomitant decrease in lignin synthesis (average decreased 0.48 and 0.53 A280 g−1). Under 20 cm deep-seeding stress, the addition of 5-HT activated the expression of multiple genes of IAA biosynthesis and signal transduction, including Zm00001d049601, Zm00001d039346, Zm00001d026530, and Zm00001d049659, and it also stimulated IAA production in both the mesocotyl and coleoptile of maize seedlings. On the contrary, 5-HT suppressed the expression of genes for lignin biosynthesis (Zm00001d016471, Zm00001d005998, Zm00001d032152, and Zm00001d053554) and retarded the accumulation of H2O2 and lignin, resulting in the elongation of the mesocotyl and coleoptile of maize seedlings. A comprehensive evaluation analysis showed that the optimum concentration of 5-HT in relieving deep-seeding stress was 2.5 mg/L for both inbred lines, and 5-HT therefore could improve the seedling emergence rate and alleviate deep-seeding stress in maize seedlings. These findings could provide a novel strategy for improving maize deep-seeding tolerance, thus enhancing yield potential under drought and water stress.
Distribution and Location of BEVs in Different Genotypes of Bananas Reveal the Coevolution of BSVs and Bananas
Authors: Rao Xueqin , Chen Huazhou , Lu Yongsi , Liu Runpei , Li Huaping
Published: December 2023

Members of the family Caulimoviridae contain abundant endogenous pararetroviral sequences (EPRVs) integrated into the host genome. Banana streak virus (BSV), a member of the genus Badnavirus in this family, has two distinct badnaviral integrated sequences, endogenous BSV (eBSV) and banana endogenous badnavirus sequences (BEVs). BEVs are distributed widely across the genomes of different genotypes of bananas. To clarify the distribution and location of BEVs in different genotypes of bananas and their coevolutionary relationship with bananas and BSVs, BEVs and BSVs were identified in 102 collected banana samples, and a total of 327 BEVs were obtained and categorized into 26 BEVs species with different detection rates. However, the majority of BEVs were found in Clade II, and a few were clustered in Clade I. Additionally, BEVs and BSVs shared five common conserved motifs. However, BEVs had two unique amino acids, methionine and lysine, which differed from BSVs. BEVs were distributed unequally on most of chromosomes and formed hotspots. Interestingly, a colinear relationship of BEVs was found between AA and BB, as well as AA and SS genotypes of bananas. Notably, the chromosome integration time of different BEVs varied. Based on our findings, we propose that the coevolution of bananas and BSVs is driven by BSV Driving Force (BDF), a complex interaction between BSVs, eBSVs, and BEVs. This study provides the first clarification of the relationship between BEVs and the coevolution of BSVs and bananas in China.
An Overview of the Use of Anti-Angiogenic Agents in the Treatment of Thymic Epithelial Tumors
Authors: Agrafiotis Apostolos C. , Berzenji Lawek , Koyen Stien , Vermeulen Dries , Winthagen Rachel , Hendriks Jeroen M. H. , Van Schil Paul E.
Published: December 2023

Angiogenesis significantly influences the carcinogenesis of thymic epithelial tumors (TET). Both thymomas and thymic carcinoma (TC) overexpress VEGF-A and VEGFR-1 and -2. This review aims to provide an appraisal of the use of anti-angiogenics in the treatment of TET. The literature research identified 16 studies that were deemed eligible for further analysis. Seven studies assessed the clinical efficacy of sunitinib and five studies the use of apatinib and/or anlotinib. The multicenter Japanese phase II REMORA trial investigated the efficacy of lenvatinib, which is a multi-targeted inhibitor of VEGFR, FGFR, RET, c-Kit, and other kinases. The objective response rate was 38% (25.6–52%), which is the highest documented in TET that progressed after first-line chemotherapy. Anti-angiogenic agents may be useful in the treatment of TET, which are not amenable to curative treatment. Their toxicity profile seems to be acceptable. However, angiogenesis inhibitors do not appear to have a major influence on either thymomas or TC, although multikinase inhibitors may have some effect on TC. The current evidence suggests that the most active agent is lenvatinib, whereas sunitinib could be proposed as an acceptable second-line therapy for TC. Further research concerning the combination of immune checkpoint inhibitors with anti-angiogenic drugs is warranted.
Circulating Tumor DNA Is a Variant of Liquid Biopsy with Predictive and Prognostic Clinical Value in Breast Cancer Patients
Authors: Zavarykina Tatiana M. , Lomskova Polina K. , Pronina Irina V. , Khokhlova Svetlana V. , Stenina Marina B. , Sukhikh Gennady T.
Published: December 2023

This paper introduces the reader to the field of liquid biopsies and cell-free nucleic acids, focusing on circulating tumor DNA (ctDNA) in breast cancer (BC). BC is the most common type of cancer in women, and progress with regard to treatment has been made in recent years. Despite this, there remain a number of unresolved issues in the treatment of BC; in particular, early detection and diagnosis, reliable markers of response to treatment and for the prediction of recurrence and metastasis, especially for unfavorable subtypes, are needed. It is also important to identify biomarkers for the assessment of drug resistance and for disease monitoring. Our work is devoted to ctDNA, which may be such a marker. Here, we describe its main characteristics and potential applications in clinical oncology. This review considers the results of studies devoted to the analysis of the prognostic and predictive roles of various methods for the determination of ctDNA in BC patients. Currently known epigenetic changes in ctDNA with clinical significance are reviewed. The possibility of using ctDNA as a predictive and prognostic marker for monitoring BC and predicting the recurrence and metastasis of cancer is also discussed, which may become an important part of a precision approach to the treatment of BC.
Gd2O3 Doped UO2(s) Corrosion in the Presence of Silicate and Calcium under Alkaline Conditions
Authors: García-Gómez Sonia , Giménez Javier , Casas Ignasi , Llorca Jordi , De Pablo Joan
Published: December 2023

The anodic reactivity of UO2 and UO2 doped with Gd2O3 was investigated by electrochemical methods in slightly alkaline conditions in the presence of silicate and calcium. At the end of the experiments, the electrodes were analysed by X-ray photoelectron spectroscopy to determine the oxidation state of the uranium on the surface. The experiments showed that the increase in gadolinia doping level led to a reduction in the reactivity of UO2, this effect being more marked at the highest doping level studied (10 wt.% Gd2O3). This behaviour could be attributed to the formation of dopant-vacancy clusters (GdIII-Ov), which could limit the accommodation of excess O2− into the UO2 lattice. In addition, the presence of Ca2+ and SiO32− decreased the anodic dissolution of UO2. In summary, the Gd2O3 doping in presence of silicate and calcium was found to strongly decrease the oxidative dissolution of UO2, which is a beneficial situation regarding the long-term management of spent nuclear fuel in a repository.
The Structure of Rice Stemborer Assemblages: A Review of Species’ Distributions, Host Ranges, and Interspecific Interactions
Authors: Horgan Finbarr G.
Published: December 2023

This review describes global rice stemborer assemblages based on published species distributions, apparent host preferences, and reported shifts in assemblage composition in response to environmental factors. At least 56 moth (Lepidoptera: Crambidae, Pyralidae, Noctuidae) and fly (Diptera: Diopsidae, Chloropidae) species have been associated with rice; however, only 21 species are of potential, large-scale economic importance with a further 2 species of localized concern; most of the remaining species’ associations with rice are based on dubious records without economic impacts on rice production. A list of stemborer–host associations indicates that rice stemborers are largely oligophagous on grasses (Poaceae), but a few species are polyphagous (also attacking Cyperaceae, Typhaceae, and some Eudicotyledon plants). Total stemborer abundance is determined by rice cropping patterns and management. Assemblage species richness is determined by geographical location, surrounding habitat (particularly as regards secondary and occasional species), and season. Evidence suggests that stemborer assemblage structure is largely determined through conditional interspecific competition. Regional assemblages typically include a single dominant lepidopteran species (primary species) that is largely restricted to rice and for which the climate is optimal; one or more secondary species that vary based on the age of rice attacked, rice anatomy, and the proximity to other habitats (including other crops); and occasional species that probably spill over from adjacent grasslands. The co-occurrence of lepidopteran with dipteran rice stemborers requires further research attention.
A Retrospective before and after Assessment of Multidisciplinary Management for Postpartum Hemorrhage
Authors: Zdanowicz Jarmila Anna , Schneider Sophie , Martignoni Carla , Lamari Salima , Fuchs Alexander , Daskalakis Michael , Surbek Daniel
Published: December 2023

Postpartum hemorrhage (PPH) remains a major cause of maternal morbidity and mortality. While PPH treatment guidelines exist, data on their effect on reduction in red blood cell (RBC) transfusions and use of hemostatic products are scarce. Continuous evaluation of PPH management is important to assess potential pitfalls and incorporate new treatment options. We retrospectively compared PPH management and administration of RBC and hemostatic products before and after international guideline implementation. The primary endpoint was RBC administration for PPH. Secondary endpoints were hemoglobin trigger for RBC administration, administration of hemostatic products and surgical therapies. In total 235 patients had a PPH, 59 in 2011 and 176 in 2018. In 2018, fewer patients received RBC within 24 h (2018: 10% vs. 2011: 32%, p < 0.001) and 24 h after delivery (2018: 4.5% vs. 2011: 37%, p < 0.001). The number of RBC units transfused per case was significantly lower in 2018 (two vs. four units in 2011, p = 0.013). A significantly reduced transfusion of fresh frozen plasma and platelets was observed in 2018 (p < 0.001 and p = 0.002, respectively). In 2011, additional surgeries for PPH in both the acute and subacute phase were performed more frequently. Local implementation of multidisciplinary PPH guidelines is feasible and was associated with a significant reduction in transfused blood products.
Intraoperative Blood Flow Analysis of Free Flaps with Arteriovenous Loops for Autologous Microsurgical Reconstruction
Authors: Geierlehner Alexander , Horch Raymund E. , Ludolph Ingo , Lang Werner , Rother Ulrich , Meyer Alexander , Arkudas Andreas
Published: December 2023

Background: Arteriovenous (AV) loops help to overcome absent or poor-quality recipient vessels in highly complex microvascular free flap reconstruction cases. There are no studies on blood flow and perfusion patterns. The purpose of this study was to evaluate and compare intraoperative hemodynamic characteristics of AV loops followed by free tissue transfer for thoracic wall and lower extremity reconstruction. Methods: this prospective clinical study combined Transit-Time Flowmetry and microvascular Indocyanine Green Angiography for the assessment of blood flow volume, arterial vascular resistance and intrinsic transit time at the time of AV loop construction and on the day of free flap transfer. Results: A total of 11 patients underwent AV loop creation, of whom five required chest wall reconstruction and six required reconstruction of the lower extremities. In seven of these cases, the latissimus dorsi flap and in four cases the vertical rectus abdominis myocutaneous (VRAM) flap was used as a free flap. At the time of loop construction, the blood flow volume of AV loops was 466 ± 180 mL/min, which increased to 698 ± 464 mL/min on the day of free tissue transfer (p > 0.1). After free flap anastomosis, the blood flow volume significantly decreased to 18.5 ± 8.3 mL/min (p < 0.001). There was no significant difference in blood flow volume or arterial vascular resistance between latissimus dorsi and VRAM flaps, nor between thoracic wall and lower extremity reconstruction. However, a significant correlation between the flap weight and the blood flow volume, as well as to the arterial vascular resistance, was found (p < 0.05). Conclusion: This is the first study to perform intraoperative blood flow and hemodynamic measurements of AV loops followed by free tissue transfer. Our results show hemodynamic differences and contribute to deeper understanding of the properties of AV loops for free flap reconstruction.
Microstructural Modification of Cold-Sprayed Ti-Cr3C2 Composite Coating by Laser Remelting
Authors: Shikalov Vladislav S. , Katanaeva Diana A. , Vidyuk Tomila M. , Golyshev Alexander A. , Kosarev Vladimir F. , Kornienko Elena E. , Malikov Alexander G. , Atuchin Victor V.
Published: December 2023

Laser processing is an effective post-treatment method for modifying the structure and improving the properties of cold-sprayed coatings. In the present work, the possibility of fabricating a hard and wear-resistant Ti-based cermet coating by cold spray followed by laser remelting was studied. A mixture of titanium and chromium carbide powders in a ratio of 60/40 wt.% was deposited by cold spray onto a titanium alloy substrate, which ensured the formation of a composite coating with a residual chromium carbide content of about 12–13 wt.%. The optimal values of laser beam power (2 kW) and scanning speed (75 mm/s) leading to the qualitative fusion of the coating with the substrate with minimal porosity and absence of defects were revealed. The microstructure and phase composition of as-sprayed and remelted coatings were examined with SEM, EDS and XRD analysis. It was shown that the phase composition of the as-sprayed coating did not change compared to the feedstock mixture, while the remelted coating was transformed into a β-Ti(Cr) solid solution with uniformly distributed nonstoichiometric TiCx particles. Due to the change in microstructure and phase composition, the remelted coating was characterized by an attractive combination of higher microhardness (437 HV0.1) and lower specific wear rate (0.25 × 10−3 mm3/N × m) under dry sliding wear conditions compared to the as-sprayed coating and substrate. Laser remelting of the coating resulted in a change in the dominant wear mechanism from oxidative–abrasive to oxidative–adhesive with delamination.
Crack-Free Joining of High-Strength AA7055 Sheets by Friction Based Self-Piercing Riveting with the Aid of Numerical Design
Authors: Huang Hui , Lim Yong Chae , Wang Yiyu , Li Yuan , Feng Zhili
Published: December 2023

Unique friction-based self-piercing riveting (F-SPR) was employed to join high-strength, low-ductility aluminum alloy 7055 for lightweight vehicle applications. This study aimed to maximize the joint strength of the AA7055 F-SPR joint while avoiding cracking issues due to low ductility at room temperature. A fully coupled Eulerian–Lagrangian (CEL) model was employed to predict the process temperature during F-SPR, and the temperature field was then mapped onto a 2D axisymmetric equivalent model for accelerated numerical analysis. The geometry, dimensions, and material strength of the rivet, as well as the depth of the die cavity and plunging depth, were investigated to enhance joint formation. Also, a static finite-element analysis model was developed to predict and analyze the stress distribution in the rivet under different mechanical testing loading conditions. Overall, the numerical model showed good agreement with the experiment results, such as joint formation and mechanical joint strength. With the aid of virtual fabrication through numerical modeling, the joint design iterations and process development time of F-SPR were greatly reduced regarding the goal of lightweight, high-strength aluminum joining.
Coastal Air Quality Assessment through AIS-Based Vessel Emissions: A Daesan Port Case Study
Authors: Yoon Jeong-Hyun , Kim Se-Won , Eom Jeong-On , Oh Jaeyong , Kim Hye-Jin
Published: December 2023

Coastal regions worldwide face increasing air pollution due to maritime activities. This technical note focuses on assessing the air pollution in the Daesan port area, Republic of Korea, using hourly emission measurements. Leveraging Automatic Identification System (AIS) data, we estimate vessel-induced air pollutant emissions and correlate them with real-time measurements. Vessel navigational statuses are categorized from the AIS data, enabling an estimation of fuel oil consumption. Random Forest models predict specific fuel oil consumption and maximum continuous ratings for vessels with unknown engine details. Using emission factors, we calculate the emissions (CO2, NO2, SO2, PM-10, and PM-2.5) from vessels visiting the port. These estimates are compared with actual air pollutant concentrations, revealing a qualitative relationship with an average correlation coefficient of approximately 0.33.
Path Planning of Unmanned Surface Vehicle Based on Improved Sparrow Search Algorithm
Authors: Liu Guangzhong , Zhang Sheng , Ma Guojie , Pan Yipeng
Published: December 2023

In order to solve the problem of many constraints and a complex navigation environment in the path planning of unmanned surface vehicles (USV), an improved sparrow search algorithm combining cubic chaotic map and Gaussian random walk strategy was proposed to plan it. Firstly, in the population initialisation stage, cubic chaotic map was used to replace the random generation method of the traditional sparrow search algorithm to optimise the uneven initial distribution of the population and improve the global search ability of the population. Secondly, in the late iteration of the algorithm, the standard deviation of fitness is introduced to determine whether the population is trapped in the local optimum. If true, the Gaussian random walk strategy is used to perturb the optimal individual and assist the algorithm to escape the local optimum. Thirdly, the chosen water environment is modelled, and the navigation information of the original inland electronic navigation chart (ENC) is preprocessed, gridised, and the obstacle swelling is processed. Finally, the path planning experiments of USV are carried out in an inland ENC grid environment. The experimental results show that, compared with the traditional sparrow search algorithm, the average fitness value of the path planned by improved sparrow search algorithm is reduced by 14.8% and the variance is reduced by 49.9%. The path planned by the algorithm is of good quality and high stability and, combined with ENC, it can provide a reliable path for USV.
The Nexus between Corporate Performance and State Ownership in Vietnam: Evidence of State Ownership’s Inverted U-Shape and Provincial Business Environment Influences
Authors: Nguyen Tran Thai Ha , Darsono Susilo Nur Aji Cokro , Phan Gia Quyen , Pham Thi Hong Van , Bach Thi Huyen , Johari Sobar M.
Published: December 2023

The level of state ownership in corporations is still a controversial topic because of its duality: on the one hand, it brings resource advantages, and on the other hand, it causes agency problems. Thus, our study aims to investigate the relationship between state ownership and corporate performance within the Vietnamese context, unraveling the impacts of state ownership’s non-linear and provincial business environment. Analyzing financial data spanning over a decade from 359 listed corporations on the Vietnamese stock markets (2010–2021), our empirical findings derived through the General Method of Moments (GMM) reveal that state ownership emerges as a potent “strategic asset” with a positive influence on corporate performance. However, a critical point is identified when state ownership surpasses the threshold of 32 percent and a decline in corporate performance ensues—a confirmation of an inverted U-shaped impact. These results substantiate the necessity of the equitization process and underscore the imperative of judiciously managing state ownership in Vietnam. Notably, our study unveils a more critical dimension: the enhanced provincial business environment bolsters corporate performance and amplifies the positive impact of state ownership. Thus, a strategic dual approach is suggested to improve corporate performance: improving the business environment and recalibrating the percentage of state shareholders. Our study serves as empirical evidence, referencing Vietnam and other transitional economies, toward mannerly policy decision-making related to state ownership and the business environment to boost corporate performance.
Predatory Responses and Feeding Behaviour of Three Elasmobranch Species in an Aquarium Setting
Authors: Costa Sandra , Neves João , Tirá Gonçalo , Andrade José
Published: December 2023

Many progressive aquariums worldwide house various elasmobranch species as part of their commitment to conservation awareness and the long-term well-being of these creatures. These aquariums face the challenge of enabling these natural predators to live harmoniously with other fish without triggering natural predation. This research, conducted at Zoomarine Algarve in Southern Portugal, aimed to investigate the behaviour of three elasmobranch species (Carcharhinus melanopterus (1:1:0), Triaenodon obesus (1:0:0), and Pteroplatytrygon violacea (0:3:0)) when exposed to different feeding mechanisms. The goal was to provide them with opportunities for alternative predatory behaviours beyond their typical feeding techniques and to reduce the likelihood of natural predation. The study took place under controlled conditions within a community habitat. Four feeding methods (pole, short buoy, long buoy, and PVC) were tested during morning, afternoon, and evening periods, using five different prey species. The results shed light on which feeding method aligns best with each species’ distinct physiological standards and predatory tendencies and revealed their prey preferences. All three species interacted with all feeding methods, with P. violacea showing a strong preference for the pole method. T. obesus favoured bony fish, while C. melanopterus showed a preference for cephalopods. P. violacea interacted with all prey types but displayed no marked preference. These various feeding methods and prey options also function as environmental enrichment strategies, enhancing the complexity of the habitat and providing the animals with more choices and control, ultimately promoting their welfare in captivity.
The Spatial Protection and Governance of Territories Based on the Ecological Product Supply: A Case Study in Beijing–Tianjin–Hebei, China
Authors: Peng Wenying , Yuchi Xiaojuan , Sun Yue , Shan Ziyi
Published: December 2023

Territory space is an ecological resource carrier and place for human development. Human activities and ecological systems are the basis of ecological product supply. Promoting territories’ spatial protection and governance by improving the supply of ecological products is very important. In this study, we established an ecological product supply capacity evaluation index system involving three types of ecological products, i.e., ecological environmental products, ecological material products, and ecological cultural products. For the case of the Beijing–Tianjin–Hebei region, we comprehensively used principal component analysis, the equivalent factor method, and the entropy method to evaluate the supply capacity of ecological products from 2011 to 2021. Then, we analyzed the spatio-temporal pattern, combining the natural breakpoint and quantile classification methods, and analyzed the obstacle factors using the obstacle degree model of ecological supply. The results show that the supply capacity of different ecological products in each city are closely related to their ecological resource endowment. The supply capacity of ecological products exhibited an upward trend, with the highest ecological environmental product supply being relatively smaller than the ecological material product supply, while the largest growth rate was for ecological cultural product supply. The supply capacity of different ecological products varied across cities over time and displayed noticeable spatial differentiation. The main obstacle factors included eco-land, eco-tourism, eco-leisure, park green space, and fishery products, although there were variations among cities. Finally, based on the level, spatial-temporal pattern, and obstacle factors of ecological product supply, we proposed strategies for territory spatial protection and governance from the perspectives of the integrated protection of elements, structural regulation, and systematic governance. The results reflected the ecological functional heterogeneity of the territory space, which can provide spatial planning guidance for sustainable development.