bluuebunny/crossref_metadata_2025 huggingface dataset:
Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from Berberis hispanica Boiss. & Reut
Authors: El Fakir Loubna , Bouothmany Kaoutar , Alotaibi Amal , Bourhia Mohammed , Ullah Riaz , Zahoor Saira , El Mzibri Mohamed , Gmouh Said , Alaoui Tajelmolk , Zaid Abdelhamid , Benbacer Laila
Published: April 2021

The current research was conducted to investigate the chemical profile, antiproliferative, and antioxidant activities of methanol extracts obtained by two different methods including maceration and Soxhlet from Berberis hispanica Boiss. & Reut. Antiproliferative activities were evaluated by the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay in four human cancer cell lines including prostate (LnCap and 22 RV1) and breast cancer (MDA-MB-231 and MCF7). The antioxidant power was evaluated by DPPH ((2,2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and FRAPS (Ferric reducing antioxidant power) tests. The chemical composition was conducted by gas chromatography-mass spectrometry (GC-MS) after methylation. Total phenolic and flavonoid contents were assessed using the Folin–Ciocalteu method. The phytochemical analysis showed that the tested extracts possessed inserting potentially active compounds. The MTT test revealed that both extracts (maceration and Soxhlet) reduced cell viability in all cell lines tested. In breast cancer cell lines MDA-MB-231 and MCF-7, the IC50 values obtained by maceration were 16.55 ± 0.58 and 17.95 ± 0.58 µg/mL, respectively. These values were slightly lower than those obtained with the Soxhlet extract toward MDA-MB-231 (19.93 ± 0.74 µg/mL) and MCF-7 (20.22 ± 0.89 µg/mL). Regarding prostate cancer cells 22 RV and LnCap, the IC50 values obtained by maceration extract (22 RV: 11.75 ± 0.35 µg/mL; LnCap: 11.91 ± 0.54 µg/mL) were also slightly lower than those obtained with Soxhlet (22 RV: 13.47 ± 0.52 µg/mL; LnCap: 19.64 ± 1.05 µg/mL). The antioxidant activity showed that the studied extracts had considerable antioxidant activity (DPPH, FRAP, and ABTS) with particular attention to the extract obtained with maceration. The Berberis hispanica Bois. and Reut. can serve society as it provides potentially bioactive compounds that may find application in the medical sector to control such diseases.
Important Roles of Oligo- and Polysaccharides against SARS-CoV-2: Recent Advances
Authors: Iravani Siavash , Varma Rajender S.
Published: April 2021

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-initiated outbreak of COVID-19 has spread rapidly around the world, posing a huge threat to public health. Natural oligo- and polysaccharides with low toxicity, good sustainability, high biocompatibility, respectable safety, immune regulation, and antiviral activity can be employed as promising candidates for the prevention and inhibition of viral infections, especially COVID-19. Glycosaminoglycans, marine polysaccharides, terrestrial plant polysaccharides, and some others have exhibited potential antiviral activity against pathogenic viruses, in the format of polysaccharide-centered vaccine adjuvants, nano-based structures, drug conveyance platforms, etc. In this review, significant recent advancements pertaining to the antiviral applications of oligo- and polysaccharides against SARS-CoV-2 are highlighted, including important challenges and future perspectives.
Advances in Search Strategy Using the Set of Brand Considerations in the Web Ecosystem
Authors: Kwon Sungeun , Kim Jonghyuk , Lee Zoonky
Published: April 2021

This study explores changes in a set of brand considerations as a result of web search strategies. Survey and personal computer log data of car buyers were used to identify online information search behavior for brands and products. Through this study, we found that higher frequencies of brand searching are associated with how much consumer-initiated sites and third-party-initiated sites are used, while lower frequencies of brand searching are only related to how much brand-initiated websites are used. We also concluded that ambivalent messages on consumer-initiated sites lead to the postponement of a decision and a continued search for another brand. In addition, third party-initiated information sources lower search costs, which lead to longer consumer journeys and expand the set of brands considered and searched. The results of this study can help marketers understand the importance of their own media and aid in the development of a digital media strategy.
Climatic Variation of Maximum Intensification Rate for Major Tropical Cyclones over the Western North Pacific
Authors: Wu Xiangbai , Yan Xiao-Hai , Li Yan , Mei Huan , Liou Yuei-An , Li Gen
Published: April 2021

To analyze the dependence of intensification rates of tropical cyclones (TCs) on the variation of environmental conditions, an index is proposed here to measure the lifetime maximum intensification rates (LMIRs) for the Saffir–Simpson scale category 4–5 TCs over the western North Pacific. To quantitatively describe the intensification rate of major TCs, the LMIR is defined as the maximum acceleration in the sustained-wind-speed over a 24-h period of an overwater TC. This new index, LMIR, is generally independent of the indices for RI frequency. The results show that the Pacific Decadal Oscillation (PDO) modulates the inter-annual relationship between the LMIR and El Niño/Southern Oscillation (ENSO). The PDO’s modulation on the ENSO’s effect on the LMIR is explored here by considering the relationship between the LMIR and the environmental conditions in different PDO phases. While the ENSO’s effect on the LMIR for the warm PDO phase is generally by affecting the variations of upper ocean heat content, ENSO mainly influences the variations of zonal wind and vertical wind shear for the cold PDO phase. Our results suggest that fast translating TCs tend to attain strong intensification during the warm PDO phase, while a warm subsurface condition may permit slow-translating TCs also to become strongly intensified during the cooling PDO phase. These findings have an important implication for both prediction of RI and the long-term projection of TC activities in the western North Pacific.
Benefits of Local Treatment Including External Radiotherapy for Hepatocellular Carcinoma with Portal Invasion
Authors: Lee Han , Park Sunmin , Seo Yeon , Yoon Won , Rim Chai ,   on behalf of the Korean Liver Cancer Study Group
Published: April 2021

We aimed to identify the oncologic benefits of local treatment including radiotherapy (LRT) in hepatocellular carcinoma (HCC) invading the portal vein. We used clinical data of patients with HCC invading the portal vein from 2008 to 2014 provided by 50 hospitals nationwide. A total of 1163 patients were included in the analysis. The LRT group was younger than the best supportive care (BSC) group (p < 0.001). The mean Child-Pugh score of the LRT group (6.1) was significantly lower than that of the BSC group (7.7) (p < 0.001). Propensity score-matched analysis generated 222 pairs. The median survival of all patients, LRT, and BSC groups were 5.0, 8.0, and 2.0 months, respectively. The overall survival (OS) rates in the LRT and BSC groups were 34.2% and 16.2% at one year, and 12.6% and 6.8% at two years, respectively (p < 0.001). Multivariate analysis showed that LRT (HR 0.41, 95% CI 0.32–0.52), age >60 years, extrahepatic metastases, tumor size ≥10 cm, and Child-Pugh class (CPC) B or C were independent predictors of higher mortality (all p < 0.05). Statistical differences in survival were maintained in all CPC-albumin-bilirubin classes (all p < 0.05). LRT was significant in patients with HCC with portal invasion, valid for patients with CPC A and B.
Oxygen Dependence of Flight Performance in Ageing Drosophila melanogaster
Authors: Privalova Valeriya , Szlachcic Ewa , Sobczyk Łukasz , Szabla Natalia , Czarnoleski Marcin
Published: April 2021

Similar to humans, insects lose their physical and physiological capacities with age, which makes them a convenient study system for human ageing. Although insects have an efficient oxygen-transport system, we know little about how their flight capacity changes with age and environmental oxygen conditions. We measured two types of locomotor performance in ageing Drosophila melanogaster flies: the frequency of wing beats and the capacity to climb vertical surfaces. Flight performance was measured under normoxia and hypoxia. As anticipated, ageing flies showed systematic deterioration of climbing performance, and low oxygen impeded flight performance. Against predictions, flight performance did not deteriorate with age, and younger and older flies showed similar levels of tolerance to low oxygen during flight. We suggest that among different insect locomotory activities, flight performance deteriorates slowly with age, which is surprising, given that insect flight is one of the most energy-demanding activities in animals. Apparently, the superior capacity of insects to rapidly deliver oxygen to flight muscles remains little altered by ageing, but we showed that insects can become oxygen limited in habitats with a poor oxygen supply (e.g., those at high elevations) during highly oxygen-demanding activities such as flight.
Overcoming TRAIL Resistance for Glioblastoma Treatment
Authors: Deng Longfei , Zhai Xuan , Liang Ping , Cui Hongjuan
Published: April 2021

The tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) shows a promising therapeutic potential in cancer treatment as it exclusively causes apoptosis in a broad spectrum of cancer cells through triggering the extrinsic apoptosis pathway via binding to cognate death receptors, with negligible toxicity in normal cells. However, most cancers, including glioblastoma multiforme (GBM), display TRAIL resistance, hindering its application in clinical practice. Recent studies have unraveled novel mechanisms in regulating TRAIL-induced apoptosis in GBM and sought effective combinatorial modalities to sensitize GBM to TRAIL treatment, establishing pre-clinical foundations and the reasonable expectation that the TRAIL/TRAIL death receptor axis could be harnessed to treat GBM. In this review, we will revisit the status quo of the mechanisms of TRAIL resistance and emerging strategies for sensitizing GBM to TRAIL-induced apoptosis and also discuss opportunities of TRAIL-based combinatorial therapies in future clinical use for GBM treatment.
Label-Free Assay of Protein Kinase A Activity and Inhibition Using a Peptide-Based Electrochemical Sensor
Authors: Cho Hyunju , Lee Chang-Seuk , Kim Tae Hyun
Published: April 2021

We propose a simple label-free electrochemical biosensor for monitoring protein kinase activity and inhibition using a peptide-modified electrode. The biosensor employs cys-kemptide (CLRRASLG) as a substrate peptide which was immobilized on the surface of a gold electrode via the self-assembly of the thiol terminals in cysteine (C) residues. The interaction between protein kinase A (PKA) and adenosine 5′-triphosphate (ATP) on the cys-kemptide immobilized electrode can cause the transfer of ATP terminal phosphates to the peptide substrates at serine (S) residues, which alters the surface charge of the electrode, thus enabling monitoring of the PKA activity via measuring the interfacial electron transfer resistance with electrochemical impedance spectroscopy. The proposed sensor showed reliable, sensitive, and selective detection of PKA activity with a wide dynamic range of 0.1–100 U/mL and a detection limit of 56 mU/mL. The sensor also exhibited high selectivity, rendering it possible to screen PKA inhibitors. Moreover, the sensor can be employed to evaluate the activity and inhibition of PKA in real samples.
Neural Correlates of a Trance Process and Alternative States of Consciousness in a Traditional Healer
Authors: Rogerson Rebecca G. , Barnstaple Rebecca E. , DeSouza Joseph FX
Published: April 2021

Trance processes are a form of altered states of consciousness (ASC) widely reported across cultures. Entering these states is often linked to auditory stimuli such as singing, chanting, or rhythmic drumming. While scientific research into this phenomenon is relatively nascent, there is emerging interest in investigating the neural correlates of altered states of consciousness such as trance. This report aims to add to this field of ASC through exploring how the perception of an experienced Sangoma (traditional South African healer) entering a trance process correlates to blood-oxygen-level-dependent (BOLD) signal modulation with auditory stimuli. Functional Magnetic Resonance Imaging (fMRI) data were analyzed using a General Linear Model comparing music versus no music condition multiplied by the percept of experiencing trance (High or Low). Positive BOLD activation was shown in the auditory cortex in both hemispheres during a trance process. Other brain regions tightly correlated to trance perception were the right parietal, right frontal, and area prostriata (p < 0.05, Bonferroni corrected). The orbitofrontal cortex (part of the Default Mode Network) was negatively activated and most correlated with music when trance was high, showing the largest differential between high and low trance perception. This is the first study to directly correlate BOLD signal variations in an expert subject’s percept of trance onset and intensity, providing insight into the neural signature and dynamics of this unique form of ASC. Future studies should examine in greater detail the perception of trance processes in expert subjects, adding other neuroimaging modalities to further investigate how these brain regions are modulated by trance expertise.
Improvement Proposal of Bottom-Up Approach for the Energy Characterization of Buildings in the Tropical Climate
Authors: Cárdenas-Rangel Jorge , Osma-Pinto German , Jaramillo-Ibarra Julián
Published: April 2021

The energy characterization of buildings can be done by bottom-up methods such as energy simulation models (samples or archetypes). A sample consists of the selection of real buildings and an archetype is a theoretical building that represents them. Nevertheless, both approaches have shortcomings for the creation of energy models. This work proposes to improve the sampling approach from the validation of input data, and calibration of models by individual adjustment processes. The studied category corresponds to multi-family buildings of median incomes from the Metropolitan Area of Bucaramanga (Colombia). This study presents the energy model of five existing buildings and an archetype, calibration results, energy characterization, and comparative analysis between both approaches. The sampling approach indicates that housing units and general services demand an average of 76.9% and 23.1% of consumed energy, respectively. The average energy consumption by housing units is 22.38 kWh/m2·year caused by appliances (85.3%), lighting (11.2%), and air conditioning (3.5%). The archetype presents similar results for the energy consumption of housing units (kWh/m2·year), but notable differences concerning a specific behavior of inner spaces, being the sampling approach more accurate to characterize to a building category.
Eco-Friendly and Sustainable Process for Converting Hydrous Bioethanol to Butanol
Authors: Landau Miron V. , Hos Tomy , Vidruk Nehemya Roxana , Nomikos George , Herskowitz Moti
Published: April 2021

The purpose of the study was the development of water-resistant catalyst and catalytic processes for the conversion of hydrous ethanol to 1-butanol. Water, in hydrous ethanol, strongly inhibits conversion to 1-butanol on solid catalysts. In this study, the nonstoichiometric P-deficient hydroxyapatite containing carbonate anions (C-HAP), Ca10−x/2(PO4)6−x(CO3)x(OH)2, displayed good performance in the Guerbet condensation of hydrated ethanol to 1-butanol, after proper stabilization of reaction conditions. Hydrous ethanol (96 wt%) was converted on C-HAP formed as extrudates with silica binder at 400 °C and weight hour space velocity (WHSV) = 0.5–1.0 h−1 to yield 21–23% 1-butanol and 73–74% selectivity. It displayed stable operation for up to 170 h on streams conducted in bench and mini-pilot rigs with catalyst loadings of 2 and 50 cm3, respectively. The process simulation employed the recycling of ethanol without laboratory verification to reach 68% theoretical yield of 1-butanol. The techno-economic analysis demonstrated the feasibility of this process, showing that it may be profitable depending on the prices of hydrated ethanol and 1-butanol.
Biomechanical Properties of Cancer Cells
Authors: Runel Gaël , Lopez-Ramirez Noémie , Chlasta Julien , Masse Ingrid
Published: April 2021

Since the crucial role of the microenvironment has been highlighted, many studies have been focused on the role of biomechanics in cancer cell growth and the invasion of the surrounding environment. Despite the search in recent years for molecular biomarkers to try to classify and stratify cancers, much effort needs to be made to take account of morphological and nanomechanical parameters that could provide supplementary information concerning tissue complexity adaptation during cancer development. The biomechanical properties of cancer cells and their surrounding extracellular matrix have actually been proposed as promising biomarkers for cancer diagnosis and prognosis. The present review first describes the main methods used to study the mechanical properties of cancer cells. Then, we address the nanomechanical description of cultured cancer cells and the crucial role of the cytoskeleton for biomechanics linked with cell morphology. Finally, we depict how studying interaction of tumor cells with their surrounding microenvironment is crucial to integrating biomechanical properties in our understanding of tumor growth and local invasion.
New Insights into the Multifaceted Role of Myeloid-Derived Suppressor Cells (MDSCs) in High-Grade Gliomas: From Metabolic Reprograming, Immunosuppression, and Therapeutic Resistance to Current Strategies for Targeting MDSCs
Authors: Lakshmanachetty Senthilnath , Cruz-Cruz Joselyn , Hoffmeyer Eric , Cole Allison P. , Mitra Siddhartha S.
Published: April 2021

Cancer cells “hijack” host immune cells to promote growth, survival, and metastasis. The immune microenvironment of high-grade gliomas (HGG) is a complex and heterogeneous system, consisting of diverse cell types such as microglia, bone marrow-derived macrophages (BMDMs), myeloid-derived suppressor cells (MDSCs), dendritic cells, natural killer (NK) cells, and T-cells. Of these, MDSCs are one of the major tumor-infiltrating immune cells and are correlated not only with overall worse prognosis but also poor clinical outcomes. Upon entry from the bone marrow into the peripheral blood, spleen, as well as in tumor microenvironment (TME) in HGG patients, MDSCs deploy an array of mechanisms to perform their immune and non-immune suppressive functions. Here, we highlight the origin, function, and characterization of MDSCs and how they are recruited and metabolically reprogrammed in HGG. Furthermore, we discuss the mechanisms by which MDSCs contribute to immunosuppression and resistance to current therapies. Finally, we conclude by summarizing the emerging approaches for targeting MDSCs alone as a monotherapy or in combination with other standard-of-care therapies to improve the current treatment of high-grade glioma patients.
Basophils Orchestrating Eosinophils’ Chemotaxis and Function in Allergic Inflammation
Authors: Iype Joseena , Fux Michaela
Published: April 2021

Eosinophils are well known to contribute significantly to Th2 immunity, such as allergic inflammations. Although basophils have often not been considered in the pathogenicity of allergic dermatitis and asthma, their role in Th2 immunity has become apparent in recent years. Eosinophils and basophils are present at sites of allergic inflammations. It is therefore reasonable to speculate that these two types of granulocytes interact in vivo. In various experimental allergy models, basophils and eosinophils appear to be closely linked by directly or indirectly influencing each other since they are responsive to similar cytokines and chemokines. Indeed, basophils are shown to be the gatekeepers that are capable of regulating eosinophil entry into inflammatory tissue sites through activation-induced interactions with endothelium. However, the direct evidence that eosinophils and basophils interact is still rarely described. Nevertheless, new findings on the regulation and function of eosinophils and basophils biology reported in the last 25 years have shed some light on their potential interaction. This review will focus on the current knowledge that basophils may regulate the biology of eosinophil in atopic dermatitis and allergic asthma.
Treatment with Bacterial Biologics Promotes Healthy Aging and Traumatic Brain Injury Responses in Adult Drosophila, Modeling the Gut–Brain Axis and Inflammation Responses
Authors: Molina Brandon , Mastroianni Jessica , Suarez Ema , Soni Brijinder , Forsberg Erica , Finley Kim
Published: April 2021

Drosophila are widely used to study neural development, immunity, and inflammatory pathways and processes associated with the gut–brain axis. Here, we examine the response of adult Drosophila given an inactive bacteriologic (IAB; proprietary lysate preparation of Lactobacillus bulgaricus, ReseT®) and a probiotic (Lactobacillus rhamnosus, LGG). In vitro, the IAB activates a subset of conserved Toll-like receptor (TLR) and nucleotide-binding, oligomerization domain-containing protein (NOD) receptors in human cells, and oral administration slowed the age-related decline of adult Drosophila locomotor behaviors. On average, IAB-treated flies lived significantly longer (+23%) and had lower neural aggregate profiles. Different IAB dosages also improved locomotor function and longevity profiles after traumatic brain injury (TBI) exposure. Mechanistically, short-term IAB and LGG treatment altered baseline nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κβ) signaling profiles in neural and abdominal tissues. Overall, at select dosages, IAB and LGG exposure has a positive impact on Drosophila longevity, neural aging, and mild traumatic brain injury (TBI)-related responses, with IAB showing greater benefit. This includes severe TBI (sTBI) responses, where IAB treatment was protective and LGG increased acute mortality profiles. This work shows that Drosophila are an effective model for testing bacterial-based biologics, that IAB and probiotic treatments promote neuronal health and influence inflammatory pathways in neural and immune tissues. Therefore, targeted IAB treatments are a novel strategy to promote the appropriate function of the gut–brain axis.
Evidence for the Management of Bronchopulmonary Dysplasia in Very Preterm Infants
Authors: Muehlbacher Tobias , Bassler Dirk , Bryant Manuel B.
Published: April 2021

Background: Very preterm birth often results in the development of bronchopulmonary dysplasia (BPD) with an inverse correlation of gestational age and birthweight. This very preterm population is especially exposed to interventions, which affect the development of BPD. Objective: The goal of our review is to summarize the evidence on these daily procedures and provide evidence-based recommendations for the management of BPD. Methods: We conducted a systematic literature research using MEDLINE/PubMed on antenatal corticosteroids, surfactant-replacement therapy, caffeine, ventilation strategies, postnatal corticosteroids, inhaled nitric oxide, inhaled bronchodilators, macrolides, patent ductus arteriosus, fluid management, vitamin A, treatment of pulmonary hypertension and stem cell therapy. Results: Evidence provided by meta-analyses, systematic reviews, randomized controlled trials (RCTs) and large observational studies are summarized as a narrative review. Discussion: There is strong evidence for the use of antenatal corticosteroids, surfactant-replacement therapy, especially in combination with noninvasive ventilation strategies, caffeine and lung-protective ventilation strategies. A more differentiated approach has to be applied to corticosteroid treatment, the management of patent ductus arteriosus (PDA), fluid-intake and vitamin A supplementation, as well as the treatment of BPD-associated pulmonary hypertension. There is no evidence for the routine use of inhaled bronchodilators and prophylactic inhaled nitric oxide. Stem cell therapy is promising, but should be used in RCTs only.
High Efficiency Fluorinated Oligo(ethylenesuccinamide) Coating for Stone
Authors: Camaiti Mara , Bortolotti Villiam , Cao Yijian , Papacchini Alessandra , Salvini Antonella , Brizi Leonardo
Published: April 2021

The protection of stone cultural assets is related to the transformation of the surface characteristic from hydrophilic to hydrophobic/superhydrophobic through the application of a coating. The suitability of a coating depends not only on its capability to dramatically change the surface wettability, but also on other parameters such as the modification of kinetics of water absorption, the permanence of vapor diffusivity, the resistance of the coating to aging and the low volatile organic compound emissions during its application. In this work, an oligo(ethylensuccinamide) containing low molecular pendant perfluoropolyether segments (SC2-PFPE) and soluble in environmentally friendly solvents was tested as a protective agent for historic stone artifacts. Magnetic resonance imaging and relaxometry were employed to evaluate the effects of the surface wettability change, to follow the water diffusion inside the rock and to study the porous structure evolution after the application of SC2-PFPE. A sun-like irradiation test was used to investigate the photo-stability of the product. The results demonstrate that the highly photo-stable SC2-PFPE minimizes the surface wettability of the stone by modifying the water sorptivity without significantly affecting its porous structure and vapor diffusivity. The improved performance of SC2-PFPE in comparison to other traditional coatings makes it a potential candidate as an advanced coating for stone cultural heritage protection.
Orbicularis Oculi Muscle Size and Function: Exploring the Influence of Aging and Exercise Training
Authors: Abe Takashi , Loenneke Jeremy P.
Published: April 2021

The orbicularis oculi muscle is the sphincter muscle of the eyelids that blinks and closes the eyes. In this review, our aim was threefold: (1) to introduce the performance characteristics of blinking activity in young and older adults, (2) to discuss the influence of aging on the orbicularis oculi muscle in healthy adults, and (3) to provide information about the effect of facial exercise training on the orbicularis oculi muscle. To achieve the purpose of this review, a search using two electronic databases (PubMed and Scopus) and a search engine (Google Scholar) was conducted. The amplitude and peak velocity of spontaneously blinking behavior, which is an index of muscle function of the orbicularis oculi, appear to be affected by aging. The muscle thickness of the orbicularis oculi tends to be low in older adults, but there are issues that need to be examined further, such as differences in sex and measurement positions. There was no study on the effect of exercise training; however, the results of a highly trained man indicate that the orbicularis oculi muscles might elicit muscle hypertrophy through non-traditional resistance exercise.
Interstitial Score and Concentrations of IL-4Rα, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial Pneumonias
Authors: Bruzova Magdalena , Pavlova Martina , Matej Radoslav , Sterclova Martina , Vasakova Martina
Published: April 2021

Idiopathic interstitial pneumonia (IIP) entails a variable group of lung diseases of unknown etiology. Idiopathic pulmonary fibrosis, nonspecific interstitial pneumonia, interstitial lung diseases related to connective tissue disease (CTD-ILD), and hypersensitivity pneumonitis (HP) can manifest with similar clinical, radiological, and histopathological features. In a differential diagnosis, biomarkers can play a significant role. We assume that levels of specific cyto- or chemokines or their receptors can signal pathogenetic processes in the lungs. Eighty patients with different types of idiopathic interstitial pneumonia were enrolled in this study. Cell counts and concentrations of tumor necrosis factor (TNF)-α, interleukin-4 receptor α, proteinase-activated receptor (PAR)-2, matrix metalloproteinase (MMP)-7, and B cell-activating factor were measured in bronchoalveolar lavage fluid using commercial ELISA kits. High resolution computer tomography results were evaluated using alveolar and interstitial (IS) score scales. Levels of TNF-α were significantly higher in HP compared to fibrosing IIP (p < 0.0001) and CTD-ILD (p = 0.0381). Concentrations of IL-4Rα, PAR-2, and MMP-7 were positively correlated with IS (p = 0.0009; p = 0.0256; p = 0.0015, respectively). Since TNF-α plays a major role in inflammation, our results suggest that HP is predominantly an inflammatory disease. From the positive correlation with IS we believe that IL-4Rα, PAR-2, and MMP-7 could serve as fibroproliferative biomarkers in differential diagnosis of IIP.
The Role and Impact of Deep Learning Methods in Computer-Aided Diagnosis Using Gastrointestinal Endoscopy
Authors: Pang Xuejiao , Zhao Zijian , Weng Ying
Published: April 2021

At present, the application of artificial intelligence (AI) based on deep learning in the medical field has become more extensive and suitable for clinical practice compared with traditional machine learning. The application of traditional machine learning approaches to clinical practice is very challenging because medical data are usually uncharacteristic. However, deep learning methods with self-learning abilities can effectively make use of excellent computing abilities to learn intricate and abstract features. Thus, they are promising for the classification and detection of lesions through gastrointestinal endoscopy using a computer-aided diagnosis (CAD) system based on deep learning. This study aimed to address the research development of a CAD system based on deep learning in order to assist doctors in classifying and detecting lesions in the stomach, intestines, and esophagus. It also summarized the limitations of the current methods and finally presented a prospect for future research.
Musculoskeletal Soft-Tissue Sarcoma: Quality Assessment of Initial MRI Reports Shows Frequent Deviation from ESSR Guidelines
Authors: Weiss Sebastian , Korthaus Alexander , Baumann Nora , Yamamura Jin , Spiro Alexander S. , Lübke Andreas M. , Frosch Karl-Heinz , Schlickewei Carsten , Priemel Matthias
Published: April 2021

Soft-tissue sarcomas (STS) are a rare subtype of soft-tissue mass and are frequently misinterpreted as benign lesions. Magnetic resonance imaging (MRI) is the primary recommended type of diagnostics. To assess the quality of primary radiology reports, we investigated whether recommended MRI report elements were included in compliance with European Society of Musculoskeletal Radiology (ESSR) guidelines. A total of 1107 patients were evaluated retrospectively, and 126 radiological reports on patients with malignant STS were assessed for ESSR quality criteria. One or more required sequences or planes were missing in 67% of the reports. In all 126 cases, the report recognized the mass as anomalous (100%). Sixty-eight percent of the reports mentioned signs of malignancy. The majority of reports (n = 109, 87%) articulated a suspected diagnosis, 32 of which showed a mismatch with the final diagnosis (25%). Thirty-two percent of the reports had a misinterpretation of the masses as benign. Benign misinterpretations were more common in masses smaller than 5 cm (65% vs. 27%). Thirty percent of the reports suggested tissue biopsy and 6% recommended referral to a sarcoma center. MRI reports showed frequent deviations from ESSR guidelines, and protocol guidelines were not routinely met. Deviations from standard protocol and reporting guidelines could put patients at risk for inadequate therapy.
Detection of SARS-COV-2 Proteins Using an ELISA Test
Authors: Di Domenico Marina , De Rosa Alfredo , Boccellino Mariarosaria
Published: April 2021

The coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable screening method is an RT-PCR test that is performed on an ororhinopharyngeal swab in the laboratory. In the current severe SARS-CoV-2 pandemic, it is necessary to identify devices for rapid diagnosis to reduce the spread of the disease. The aim of this study was to provide a qualitative, rapid, sensitive, and specific method for a diagnosis of SARS-CoV-2 infection based on the recognition of specific antigens of the SARS-CoV-2 virus. The device was built by assembling commercially available and custom-made semi-finished products. The method was performed in environments outside the laboratory, i.e., “patient side,” with an immediate chemocolorimetric response or with a digital reader using an ELISA method.
A New Method for High Resolution Surface Change Detection: Data Collection and Validation of Measurements from UAS at the Nevada National Security Site, Nevada, USA
Authors: Crawford Brandon , Swanson Erika , Schultz-Fellenz Emily , Collins Adam , Dann Julian , Lathrop Emma , Milazzo Damien
Published: April 2021

The use of uncrewed aerial systems (UAS) increases the opportunities for detecting surface changes in remote areas and in challenging terrain. Detecting surface topographic changes offers an important constraint for understanding earthquake damage, groundwater depletion, effects of mining, and other events. For these purposes, changes on the order of 5–10 cm are readily detected, but sometimes it is necessary to detect smaller changes. An example is the surface changes that result from underground explosions, which can be as small as 3 cm. Previous studies that described change detection methodologies were generally not aimed at detecting sub-5-cm changes. Additionally, studies focused on high-fidelity accuracy were either computationally modeled or did not fully provide the necessary examples to highlight the usability of these workflows. Detecting changes at this threshold may be critical in certain applications, such as global security research and monitoring for high-consequence natural hazards, including landslides. Here we provide a detailed description of the methodology we used to detect 2–3 cm changes in an important applied research setting—surface changes related to underground explosions. This methodology improves the accuracy of change detection data collection and analysis through the optimization of pre-field planning, surveying, flight operations, and post-processing the collected data, all of which are critical to obtaining the highest output data resolution possible. We applied this methodology to a field study location, collecting 1.4 Tb of images over the course of 30 flights, and location data for 239 ground control points (GCPs). We independently verified changes with orthoimagery, and found that structure-from-motion, software-reported root mean square errors (RMSEs) for both control and check points underestimated the actual error. We found that 3 cm changes are detectable with this methodology, thereby improving our knowledge of a rock’s response to underground explosions.
Mobility Restrictions and E-Commerce: Holistic Balance in Madrid Centre during COVID-19 Lockdown
Authors: Villa Rafael , Monzón Andrés
Published: April 2021

COVID-19 has brought about a substantial change in urban mobility, as well as an unprecedented increase in e-commerce throughout the world due to the emergence of new ways of shopping and consumption habits. In this context, urban logistics plays a crucial role in the triple bottom line of sustainability. The present document establishes a holistic vision of the problem aiming to (i) measure and compare the traffic generated in the Madrid Central area (low-emission zone) during the periods before and after the pandemic, and (ii) quantify e-commerce orders made by residents, as well as the Light Commercial Vehicles (LCV) required to deliver these parcels, measuring their environmental impact. The results show that road traffic in the Madrid Central area decreased by approximately 2/3 compared to normal levels and 1/2 in the case of LCVs. With regards to e-commerce, the number of parcels delivered doubled. This fact entailed an increase in the number of LVCs dedicated to package delivery in the central district and more pollution, but to a lesser extent than the growth of e-commerce. The challenge faced by urban logistics in the post-Covid era is managing to blend new mobility within large cities with the high volumes of e-commerce deliveries demanded by residents.
Design and Validation of a Questionnaire on Influence of the University Classroom on Motivation and Sociability
Authors: González-Zamar Mariana-Daniela , Jiménez Luis Ortiz , Ayala Adoración Sánchez
Published: April 2021

In recent years, the changes experienced in the educational and social field have been reflected in the growing interest in defining how the attributes of the learning space affect students. This study seeks to determine the influence exerted by the design and organization of the university classroom on motivation and social interaction. The objective was to develop and validate a measurement instrument to evaluate the impact of the university classroom design on the student, considering their physical, environmental, perceptual, and motivational attributes. The questionnaire was applied to a sample of 402 students from the University of Almeria (Spain). The reliability and content validity of the construct were analyzed. Reliability was demonstrated by a Cronbach’s alpha of 0.938 for the dimensions with quantitative cut items, being highly satisfactory. The Chi-square goodness of fit test analyzed the qualitative items. Content validity was subject to expert judgment. It is concluded that the presented instrument meets the required technical attributes, to be considered a reliable and valid educational tool for measurement.
Accurate Determination of Conversion Gains of SVOM VT CCDs Based on a Signal-Dependent Charge-Sharing Mechanism
Authors: Pan Yue , Fan Xuewu , Wang Hu , Zhao Hui , Qiu Yulei , Gao Wei , Zhang Jian
Published: April 2021

The signal-variance method and the photon transfer curve method are the most valuable tools for calculating the conversion gains of charge-coupled device (CCD) detectors. This paper describes the phenomena that arise in the conversion gain measurements of space multi-band variable object monitor (SVOM) visible telescope (VT) CCDs, where the results of the signal-variance method increase with the image gray level, and the results of the photon transfer curve method appear with nonlinearity, which is caused by the signal-dependent charge sharing mechanism of back-illuminated CCDs. A numerical simulation model based on random variables was adopted to analyze the influence of the mechanism on the gain determination. The model simulates all the signals and noise in the flat field image, including the photon signal and photon-shot noise, readout noise, fixed pattern noise, and the signal-dependent charge-sharing signal, and it demonstrated agreement with the experimental data. Then, we proposed a quadratic polynomial curve-fitting formula for the photon transfer curve, and we quantitatively analyzed the relationship between the fitting coefficients and the gain, the signal-dependent charge sharing coefficient, and the full well capacity using the control variable method. Finally, the formula was used to accurately determine the conversion gains of SVOM VT CCDs.
A Modified Dynamic Model of Single-Sided Linear Induction Motors Considering Longitudinal and Transversal Effects
Authors: Heidari Hamidreza , Rassõlkin Anton , Razzaghi Arash , Vaimann Toomas , Kallaste Ants , Andriushchenko Ekaterina , Belahcen Anouar , Lukichev Dmitry V.
Published: April 2021

This paper proposes a modified dynamic equivalent circuit model for a linear induction motor considering both longitudinal end effect and transverse edge effect. The dynamic end effect (speed-dependent end effect) is based on conventional Duncan’s approach. The transverse edge effect is investigated by using three correction factors applied to the secondary resistance and magnetizing inductance. Moreover, the iron saturation effect, the skin effect, and the air-gap leakage effect are incorporated into the proposed model by using the field-analysis method. A new topology of the steady-state and space-vector model of linear induction, regarding all mentioned phenomena, is presented. The parameters of this model are calculated using both field analysis and the finite-element method. The steady-state performance of the model is first validated using the finite-element method. Additionally, the dynamic performance of the proposed model is studied. The results prove that the proposed equivalent circuit model can precisely predict the dynamic and steady-state performances of the linear induction.
Oxy-Fuel Combustion of Hard Coal, Wheat Straw, and Solid Recovered Fuel in a 200 kWth Calcium Looping CFB Calciner
Authors: Moreno Joseba , Hornberger Matthias , Schmid Max , Scheffknecht Günter
Published: April 2021

The fluidized bed combustion (FBC) of biomass and solid recovered fuel (SRF) is globally emerging as a viable solution to achieve net-negative carbon emissions in the heat and power sector. Contrary to conventional fossil fuels, alternative fuels are highly heterogeneous, and usually contain increased amounts of alkaline metals and chlorine. Hence, experimental studies are mandatory in order to thoroughly characterize the combustion behavior and pollutant formation of non-conventional fuels in novel applications. This work gives an overview of experimental investigations on the oxy-fuel combustion of hard coal, wheat straw, and SRF with a limestone bed in a semi-industrial circulating fluidized bed (CFB) pilot plant. The CFB combustor was able to be operated under different fuel blending ratios and inlet O2 concentrations, showing a stable hydrodynamic behavior over many hours of continuous operation. The boundary conditions introduced in this study are expected to prevail in carbon capture and storage (CCS) processes, such as the oxy-fuel combustion in the CFB calciner of a Calcium Looping (CaL) cycle for post-combustion CO2 capture.
A Data Driven Approach to Robust Event Detection in Smart Grids Based on Random Matrix Theory and Kalman Filtering
Authors: Han Fujia , Ashton Phillip M. , Li Maozhen , Pisica Ioana , Taylor Gareth , Rawn Barry , Ding Yi
Published: April 2021

Increasing levels of complexity, due to growing volumes of renewable generation with an associated influx of power electronics, are placing increased demands on the reliable operation of modern power systems. Consequently, phasor measurement units (PMUs) are being rapidly deployed in order to further enhance situational awareness for power system operators. This paper presents a novel data-driven event detection approach based on random matrix theory (RMT) and Kalman filtering. A dynamic Kalman filtering technique is proposed to condition PMU data. Both simulated and real PMU data from the transmission system of Great Britain (GB) are utilized in order to validate the proposed event detection approach and the results show that the proposed approach is much more robust with regard to event detection when applied in practical situations.
Precise Method to Estimate the Herschel-Bulkley Parameters from Pipe Rheometer Measurements
Authors: Magnon Elie , Cayeux Eric
Published: April 2021

Accurate characterization of the rheological behavior of non-Newtonian fluids is critical in a wide range of industries as it governs process efficiency, safety, and end-product quality. When the rheological behavior of fluid may vary substantially over a relatively short period of time, it is desirable to measure its viscous properties on a more continuous basis than relying on spot measurements made with a viscometer on a few samples. An attractive solution for inline rheological measurements is to measure pressure gradients while circulating fluid at different bulk velocities in a circular pipe. Yet, extracting the rheological model parameters may be challenging as measurement uncertainty may influence the precision of the model fitting. In this paper, we present a method to calibrate the Herschel-Bulkley rheological model to a series of differential pressure measurements made at variable bulk velocities using a combination of physics-based equations and nonlinear optimization. Experimental validation of the method is conducted on non-Newtonian shear-thinning fluid based on aqueous solutions of polymers and the results are compared to those obtained with a scientific rheometer. It is found that using a physics-based method to estimate the parameters contributes to reducing prediction errors, especially at low flow rates. With the tested polymeric fluid, the proportion difference between the estimated Herschel-Bulkley parameters and those obtained using the scientific rheometer are −24% for the yield stress, 0.26% for the consistency index, and 0.30% for the flow behavior index. Finally, the computation requires limited resources, and the algorithm can be implemented on low-power devices such as an embedded single-board computer or a mobile device.
Transcriptomic Profiling of Fe-Responsive lncRNAs and Their Regulatory Mechanism in Rice
Authors: Wang Shoudong , Sun Shuo , Guo Runze , Liao Wenying , Shou Huixia
Published: April 2021

Iron (Fe) deficiency directly affects crop growth and development, ultimately resulting in reduced crop yield and quality. Recently, long non-coding RNAs (lncRNAs) have been demonstrated to play critical regulatory roles in a multitude of pathways across numerous species. However, systematic screening of lncRNAs responding to Fe deficiency and their regulatory mechanism in plants has not been reported. In this work, 171 differently expressed lncRNAs (DE-lncRNAs) were identified based on analysis of strand-specific RNA-seq data from rice shoots and roots under Fe-deficient conditions. We also found several lncRNAs, which could generate miRNAs or act as endogenous target mimics to regulate expression of Fe-related genes. Analysis of interaction networks and gene ontology enrichment revealed that a number of DE-lncRNAs were associated with iron transport and photosynthesis, indicating a possible role of lncRNAs in regulation of Fe homeostasis. Moreover, we identified 76 potential lncRNA targets of OsbHLH156, a key regulator for transcriptional response to Fe deficiency. This study provides insight into the potential functions and regulatory mechanism of Fe-responsive lncRNAs and would be an initial and reference for any further studies regarding lncRNAs involved in Fe deficiency in plants.