bluuebunny/crossref_metadata_2025 huggingface dataset:
Stakeholders y outcomes no intencionados en la comunicación estratégica
Authors: López del Castillo Wilderbeek Francisco I.
Published: June 2020

Los resultados no deseados de las acciones de comunicación se conocen como outcomes no intencionados según los modelos de evaluación más extendidos. La medición de estos outcomes adquiere enorme relevancia en tanto pueden representar efectos adversos para los grupos de interés (stakeholders) que están vinculados a la organización: esta correlación pone en peligro los objetivos comunicativos y puede impactar negativamente en la actividad. En este artículo se analiza el resultado no deseado de una acción de comunicación estratégica. De manera más específica, se propone un análisis de contenido de la aparición de críticas en los medios a las políticas engañosas sobre compromiso medioambiental (greenwashing) durante la celebración de la Conferencia de las Naciones Unidas sobre Cambio Climático (COP25) realizada en Madrid en 2019. A través de la observación cuantitativa de la cobertura mediática y la cuantificación de las variables extraídas, se llega a definir la verdadera implicación mediática de la perspectiva del greenwashing y, específicamente, su afectación en los potenciales stakeholders.
Comunicación corporativa para la fidelización del cliente
Authors: Durán Bravo Patricia , Cisneros Martínez Nancy , Pancardo Peralta Raúl
Published: June 2020

La propuesta del modelo estratégico para diagnosticar la comunicación corporativa orientada a mejorar la fidelización del cliente fue desarrollada a partir de tres genealogías: la comprensión de la complejidad del contexto organizacionalde Scheinsohn (2009), el Branding Corporativo de Capriotti (2009) y el Proceso Comercial Relacional de Chiesa (2005). Con base en estas propuestas teóricas, se propone un Modelo estratégico de diagnóstico de la comunicacióncorporativa, con un paquete metodológico que permita identificar la carga informativa, relacional y simbólica, para orientar estratégicamente los activos intangibles como la identidad, la imagen y la reputación de la organización hacia los programas de atracción, satisfacción y fidelización del cliente.
PENGARUH SERVQUAL TERHADAP KEPUASAN YANG BERDAMPAK PADA LOYALITAS PENGGUNA E-WALLET (OVO)
Authors: Foster Bob
Published: June 2020

The purpose of this study is to analyze the direct effect of service quality on satisfaction, the direct effect of service quality and satisfaction on loyalty, the direct effect of satisfaction on loyalty, and the indirect effect of service quality on the loyalty of the users of e-wallet or digital wallet application (Ovo). The research sample consisted of 100 respondents taken by convenience random sampling technique. This research used a descriptive verification method. Data were analyzed using structural equation modeling run by Lisrel 8.80 software. The results showed that service quality and satisfaction had a positive and significant effect on loyalty, service quality had a positive and significant effect on satisfaction, and service quality had a positive and significant effect on loyalty as mediated by satisfaction. These results indicate that increasing service quality will increase consumer satisfaction, which finally increases consumer loyalty.

Keywords: loyalty, satisfaction, service quality
Reflections of Transformation
Authors: Rushing Alison , Gee Rose Mary , Dubert Christy J. , Hamilton Ellen K.
Published: June 2020

This poem was extracted from a larger study exploring the experience of serenity in persons recovering from alcohol/drug addiction in 12-step programs. The piece was shaped using the search for serenity through the eyes of a person in recovery. It maintains the storytelling tradition of 12-step programs while retaining the unique experience of the storyteller through her own eyes. It reflects her struggle toward sobriety from the self-loathing of a person in the throes of addiction—the person in the mirror that she was ashamed of—to the acceptance of herself as reflected in growth, self-respect, and self-love.
PROCEEDING OF CHEMISTRY CONFERENCES VOL 4 (2019)
Authors: Kimia UNS Departement
Published: June 2020

NATIONAL SEMINAR OF CHEMISTRY 2018
An Analysis of Parameter Invariance according to Different Sample Sizes and Dimensions in Parametric and Nonparametric Item Response Theory
Authors: REYHANLIOĞLU Çiğdem , DOĞAN Nuri
Published: June 2020

The aim of this study is to analyze the invariance of the estimated parameters in regard to Item Response Theory (IRT) applications within different dimensionality and sample characteristics. To realize this aim, students' answers in test booklet 'A' of the first stage of TEOG, Secondary Education Placement Test applied by the Ministry of Education in Turkey, in 2015 were used as the data. The population size of the study was determined as 63,871. The groups of 50, 100, 200, 500, 1000 and 5000 people who were randomly selected from the population comprised the sample of the study. One-dimensional mathematics subtest and artificially formed two-dimensional test results were used within IRT applications. As a result of the findings obtained from the study within the analysis of the one-dimensional test in regard to one-dimensional nonparametric IRT (ODNPIRT), item parameter invariance was obtained from the sample size 200. When it was analyzed in regard to one-dimensional parametric IRT (ODPIRT), it was found that at least a sample size of 1000 has to be used for item parameter estimations nearly as high as the population value (parameter). It was found that invariance for item parameters was not obtained by analyzing the two-parameter test in regard to ODPIRT, ODNPIRT and Multidimensional IRT (MDIRT).
Epidemiology, vaccine effectiveness, and risk factors for mortality for pneumococcal disease among hospitalised adults in Singapore: a case-control study
Authors: Chan Tyson , Tay Min Zhi , Kyaw Win Mar , Chow Angela , Ho Hanley J
Published: June 2020

Background: Streptococcus pneumoniae infections can lead to severe morbidity and mortality, especially in patients with invasive pneumococcal disease (IPD). This study evaluated factors associated with pneumococcal disease, pneumococcal vaccine effectiveness, and risk factors for all-cause mortality in hospitalised adults with pneumococcal disease in Singapore. Methods: Retrospective case-control study of patients tested for pneumococcal disease with streptococcal urinary antigen testing and at least one sterile site culture, during their admission to a tertiary hospital in Singapore from 2015-2017. Patients were defined as cases of IPD or non-IPD, or as controls, based on laboratory results and clinical diagnoses. Multivariable models were constructed to determine factors associated with IPD/non-IPD, and risk factors for mortality from pneumococcal disease. Vaccine effectiveness against IPD/non-IPD was estimated using a variation of the test-negative design. Results: We identified 496 pneumococcal disease cases, of whom 92 (18.5%) had IPD. The mean age of cases was 69.1±15.4yrs, and 65.5% were male. Compared with controls (N=9,181), IPD patients were younger (mean age 61.5±16.3yrs, vs 72.2±16.1yrs in controls; p<0.001) and with less co-morbidities [median Charlson’s score 1 (IQR 0-4), vs 3 (1-5) in controls; p<0.001]. IPD patients also had the highest proportions with intensive care unit (ICU) admission (20.7%), inpatient mortality (26.1%) and longest median length of stay [9 (IQR 8-17) days]. On multivariable analysis, IPD was negatively associated with prior pneumococcal vaccination (adjusted relative risk ratio=0.20, 95%CI 0.06–0.69; p=0.011). Risk factors for mortality among pneumococcal disease patients were ICU admission, diagnosis of IPD, age ≥85yrs and Charlson’s score >3. Conclusion: Patients with pneumococcal disease (especially IPD) were younger and had less co-morbidities than controls, but had higher risk of severe clinical outcomes and mortality. Pneumococcal vaccination effectivenessagainst IPD was estimated to be about 80%, and should be encouraged among high-risk patients.
Immigration, acculturation, and preferred help-seeking sources for depression. Comparison of five ethnic groups
Authors: Markova Valeria , Sandal Gro Mjeldheim , Pallesen Ståle
Published: June 2020

Background: Immigrants are more likely than the majority population to have unmet needs for public mental health services. This study aims to understand potential ethnic differences in preferred help-seeking sources for depression in Norway, and how such preferences relate to acculturation orientation. Methods: A convenience sample of immigrants from Russia ( n =164), Poland ( n =127), Pakistan ( n =128), and Somalia ( n =114), and Norwegian students ( n =250) completed a survey. The sample was recruited from social media platforms, emails, and direct contact. The survey consisted of a vignette describing a moderately depressed person. Respondents were asked to provide advice to the person by completing a modified version of the General Help-Seeking Questionnaire. The immigrant sample also responded to questions about acculturation orientation using the Vancouver Index of Acculturation Scale. Results: Significant differences were found in the endorsement of traditional (e.g., religious leader), informal (e.g., family), and semiformal (e.g., internet forum) help-sources between immigrant groups, and between immigrant groups and the Norwegian respondent group. Immigrants from Pakistan and Somalia endorsed traditional help sources to a greater extent than immigrants from Russia and Poland, and the Norwegian student sample. There were no ethnic differences in endorsement of formal mental help sources (e.g., a medical doctor). Maintenance of the culture of origin as the acculturation orientation was associated with preferences for traditional and informal help sources, while the adoption of mainstream culture was associated with semiformal and formal help-seeking sources. Conclusion: Ethnic differences in help-seeking sources need to be considered when designing and implementing mental health services.
Plant defensin antibacterial mode of action against Pseudomonas species
Authors: Sathoff Andrew E. , Lewenza Shawn , Samac Deborah A.
Published: June 2020

Background: Though many plant defensins exhibit antibacterial activity, little is known about their antibacterial mode of action (MOA). Antimicrobial peptides with a characterized MOA induce the expression of multiple bacterial outer membrane modifications, which are required for resistance to these membrane-targeting peptides. Mini-Tn 5-lux mutant strains of Pseudomonas aeruginosa with Tn insertions disrupting outer membrane protective modifications were assessed for sensitivity against plant defensin peptides. These transcriptional lux reporter strains were also evaluated for lux gene expression in response to sublethal plant defensin exposure. Also, a plant pathogen, Pseudomonas syringae pv. syringae was modified through transposon mutagenesis to create mutants that are resistant to in vitro MtDef4 treatments. Results: Plant defensins displayed specific and potent antibacterial activity against strains of P. aeruginosa . A defensin from Medicago truncatula , MtDef4, induced dose-dependent gene expression of the aminoarabinose modification of LPS and surface polycation spermidine production operons. The ability for MtDef4 to damage bacterial outer membranes was also verified visually through fluorescent microscopy. Another defensin from M. truncatula , MtDef5, failed to induce lux gene expression and limited outer membrane damage was detected with fluorescent microscopy. The transposon insertion site on MtDef4 resistant P. syringae pv. syringae mutants was sequenced, and modifications of ribosomal genes were identified to contribute to enhanced resistance to plant defensin treatments. Conclusions: MtDef4 damages the outer membrane similar to polymyxin B, which stimulates antimicrobial peptide resistance mechanisms to plant defensins. MtDef5, appears to have a different antibacterial MOA. Additionally, the MtDef4 antibacterial mode of action may also involve inhibition of translation.
Genome-wide identification of the 14-3-3 gene family and its participation in response to floral transition by interacting with TFL1/FT in Apple
Authors: Zuo Xiya , Wang Shixiang , Xiang Wen , Yang Huiru , Tahir Muhammad Mobeen , Zhen Shangong , An Na , Han Mingyu , Zhao Caiping , Zhang Dong
Published: June 2020

Background: Apple ( Malus domestica Borkh.) is one of the most popular cultivated fruit crops in China. Apple floral transition is an important process but liable to be affected by various environmental factors. The 14-3-3 proteins are involved in regulating diverse biological processes in plants, and some 14-3-3 members have been identified to play vital roles in flowering. However, little information was available about the 14-3-3 members in apple. Results: In the current study, we identified eighteen 14-3-3 gene family members from apple genome database, designated MdGF14a to MdGF14r . The isoforms possess a conserved core region composed of nine antiparallel α-helices and divergent N and C termini. According to their structural and phylogenetic features, Md14-3-3s classified into two major evolutionary branches, the epsilon (ɛ) group and the non-epsilon (non-ɛ) group. Moreover, expression profiles derived from transcriptome data and quantitative reverse-transcription PCR (qRT-PCR) analysis exhibited diverse expression patterns of Md14-3-3 genes in various tissues and in response to different sugars and hormones treatments during floral transition phase. Four Md14-3-3 isoforms ( MdGF14a , MdGF14d , MdGF14i and MdGF14j ) exhibiting prominent transcriptional responses to sugars and hormones were selected for further investigation. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation (BiFC) experiments showed that the four Md14-3-3 proteins interact with key floral integrators, MdTFL1 (TERMINAL FLOWER1) and MdFT (FLOWERING LOCUS T). Subcellular localization of four selected Md14-3-3 proteins demonstrated their localization in both the cytoplasm and nucleus. Conclusion: We comprehensively identified Md14-3-3s family in apple. Some Md14-3-3 genes are predominantly expressed during apple floral transition stage, and may participate in regulation of flowering through association with flower control genes. Our results provide a preliminary framework for further investigation into the roles of Md14-3-3s for floral transition.
Intrinsic Patient Factors Predictive of Inpatient Rehabilitation Facility Discharge Following Primary Total Knee Arthroplasty: A Systematic Review and Meta-Analysis.
Authors: Sattler Larissa Nicole , Hing Wayne Anthony , Rathbone Evelyne , Vertullo Christopher John
Published: June 2020

Background Total Knee Arthroplasty (TKA) reduces pain and improves function in those suffering from severe osteoarthritis. A significant cost of TKA is post-acute care, however, current evidence suggests that discharge to an Inpatient Rehabilitation Facility (IRF) has inferior outcomes to home discharge, with no greater benefit in physical function. Only individual studies have investigated TKA patient characteristics predictive of discharge destination, therefore, the aim is to systematically review the literature and meta-analyse intrinsic patient factors predictive of IRF discharge. If predictive factors are known, then early discharge planning and intervention strategies could be implemented. Methods Databases PubMed, CINAHL, Embase, Cochrane, and Pedro were searched up to October 2019 for all studies investigating pre-operative intrinsic patient factors predictive of IRF discharge. For assessing the methodological quality of included studies, the Quality In Prognosis Studies (QUIPS) tool was used. Statistical analysis and graphical reporting were conducted in R statistical software. To assess the effect of predictors of discharge destination, odds ratios with the corresponding 95%CI were extracted from the results of univariate and multivariable analyses. Results A total of 9 articles published between 2011 to 2018 with 218,151 TKA patients were included. Of the 13 intrinsic patient factors reported, 6 met the criteria for synthesised review: age, obesity, comorbidity, gender, SF-12/VR-12 survey, and smoking. Due to the heterogeneity of statistical analysis and reporting 2 variables could undergo meta-analysis, gender and smoking. Female gender increased the likelihood of IRF discharge by 78% (OR=1.78; 95%CI=1.43–2.20; I2=33.3%), however, the relationship between smoking status and discharge destination was less certain (OR=0.80; 95%CI=0.42–1.50; I2=68.5%). Conclusion In this systematic literature review and meta-analysis female gender was shown to be predictive of IRF discharge after total knee arthroplasty. There was also a trend for those of older age and increased comorbidity, as measured by the Charlson Comorbidity Index, or the severely obese to have an increased likelihood of IRF discharge. The marked heterogeneity of statistical methods and reporting in existing literature made pooled analysis challenging for intrinsic patient factors predictive of IRF discharge after TKR. Further, high quality studies of prospective design on predictive factors are warranted, to enable early discharge planning and optimise resource allocation on post-acute care following TKA.Trial Registration: This review was registered with PROSPERO (CRD42019134422)
Plant and mouse EB1 proteins have opposite intrinsic properties on the dynamic instability of microtubules.
Authors: Molines Arthur T , Stoppin-Mellet Virginie , Arnal Isabelle , Coquelle Frédéric M
Published: June 2020

Objective Most eukaryotic cells contain microtubule filaments, which play central roles in intra-cellular organization. However, microtubule networks have a wide variety of architectures from one cell type and organism to another. Nonetheless, the sequences of tubulins, of Microtubule Associated proteins (MAPs) and the structure of microtubules are usually well conserved throughout the evolution. MAPs being known to be responsible for regulating microtubule organization and dynamics, this raises the question of the conservation of their intrinsic properties. Indeed, knowing how the intrinsic properties of individual MAPs differ between organisms might enlighten our understanding of how distinct microtubule networks are built. End-Binding protein 1 (EB1), first described as a MAP in yeast, is conserved in plants and mammals. The intrinsic properties of the mammalian and the yeast EB1 proteins have been well described in the literature but, to our knowledge, the intrinsic properties of EB1 from plant and mammals have not been compared thus far.Results Here, using an in vitro assay, we discovered that plant and mammalian EB1 purified proteins have different intrinsic properties on microtubule dynamics. Indeed, the mammalian EB1 protein increases microtubules dynamic while the plant EB1 protein stabilizes them.
Risk factors for postoperative delirium in patients with triple-branched stent graft implantation
Authors: Lin Yanjuan , Chen Qiong , Zhang Haoruo , Chen Liang-Wan , Peng Yanchun , Huang Xizhen , Chen Yiping , Li Sailan , Lin Lingyu
Published: June 2020

Background: Neurological complications is a common complication following novel triple-branched stent graft implantation in patients with Stanford type A aortic dissection (AAD). But the incidence and risk factors of postoperative delirium (POD) are not completely clear. The aim of this study was to investigate the incidence and risk factors of POD after novel triple-branched stent graft implantation.Methods: An observational study of AAD patients who underwent novel triple-branched stent graft implantation between January 2017 and July 2019 were followed up after surgery. Patients’ delirium was screened by the Richmond Agitation-Sedation Scale and the Confusion Assessment Method for the intensive care unit from the first day after the operation, lasted 5 days. The risk factors of POD were analyzed by the Cox proportional hazard models.Results: A total of 280 AAD patients were enrolled in this research, the incidence of POD was 37.86%. Adjusting for age, body mass index, and mechanical ventilation duration, multivariate Cox regression analysis model revealed that non-manual work (adjusted hazard ratio [AHR] = .554; 95% CI: 0.335-0.915; P = .021), Acute Physiology and Chronic Health Evaluation II (APACHE-II) scores > 20 (AHR = 3.359, 95% CI: 1.707-6.609, P < .001), hypoxemia (AHR = 1.846, 95% CI: 1.118-3.048, P = .017), and more than two types of analgesics and sedatives were independently associated with POD.Conclusions: This study showed that risk factors independently associated with POD were APACHE-II score > 20, hypoxemia, and more types of analgesics and sedatives, and non-manual work was the protective factor.Trial registration: This study was retrospectively registered in the Chinese Clinical Trial Registry (Registration number: ChiCTR1900022408; Date: 2019/4/10)
Life negative events and depressive symptoms: the China Longitudinal Ageing Social Survey
Authors: Wang Zhenjie , Yang Hanmo , Zheng Pianpian , Liu Bei , Guo Zhanyuan , Geng Shen , Hong Shenda
Published: June 2020

Background: Although some studies have reported the association between life negative events and depressive disorders, very limited studies have examined the association between life negative events exposure and depressive symptoms risk among Chinese older adults.Methods: Data were obtained from the China Longitudinal Ageing Social Survey (CLASS), which was a stratified, multi-stage, probabilistic sampling survey, conducted in 2014. General linear regression and logistic regression were used to examine the association between life negative events exposure and depressive symptoms among Chinese older adults.Results: Life negative events showed statistical dose-response association with depressive symptoms risk after adjustment for the confounding factors (P trend <0.001). Under consideration of life negative events exposure, participants who lived in rural areas, without a spouse or live alone were vulnerable to depressive symptoms.Conclusions: Life negative events played a risk role of depressive symptoms among Chinese older adults, especially among those in rural areas, females or without a spouse. Our current study is valuable for the development of special prevention depressive symptoms programs among elderly individuals, especially those who have experienced negative events.
The Polyphenol-Rich Extract from Chokeberry (Aronia melanocarpa L.) Modulates Gut Microbiota and Improves Lipid Metabolism in Diet-Induced Obese Rats
Authors: Zhu Yue , Zhang Jia-ying , Wei Yu-long , Hao Jing-yi , Lei Yu-qing , Sun Ai-dong
Published: June 2020

The gut microbiota plays a critical role in obesity and lipid metabolism disorder. Chokeberry (Aronia melanocarpa L.) are rich in polyphenols with various physiological and pharmacological activities. We determined serum physiological parameters and fecal microbial components by using related kits, liquid chromatography-mass spectrometry (LC-MS) and 16S rRNA gene sequencing every 10 days. Real-time PCR analysis was used to measure gene expression of bile acids (BAs) and lipid metabolism in liver and adipose tissues. We analyzed the effects of different Chokeberry polyphenol (CBPs) treatment time on obesity and lipid metabolism in high fat diet (HFD)-fed rats. The results indicated that CBPs treatment prevents obesity, liver steatosis and improves dyslipidemia in HFD-fed rats. CBPs modulated the composition of the gut microbiota with the extended treatment time, reducing the Firmicutes/Bacteroidetes ratio (F/B ratio) and increasing the relative abundance of Bacteroides, Prevotella, Akkermansia and other bacterial species associated with anti-obesity properties. We found that CBPs treatment gradually decreased the total BAs pool and particularly reduced the relative content of cholic acid (CA), deoxycholic acid (DCA) and enhanced the relative content of chenodeoxycholic acid (CDCA). These changes were positively correlated Bacteroides, Prevotella and negatively correlated with Clostridium, Eubacterium, Ruminococcaceae. In liver and white adipose tissues, the gene expression of lipogenesis, lipolysis and BAs metabolism were regulated after CBPs treatment in HFD-fed rats, which was most likely mediated through FXR and TGR-5 signaling pathway to improve lipid metabolism. In addition, the mRNA expression of PPARγ, UCP1 and PGC-1α were upregulated markedly in interscapular brown adipose tissue (iBAT) after CBPs treatment. We confirmed that CBPs could reduce the body weight of HFD-fed rats by accelerating energy homeostasis and thermogenesis in iBAT. Finally, the fecal microbiota transplantation (FMT) experiment results demonstrated that FMT from CBPs-treated rats failed to reduce the weight of HFD-fed rats. However, FMT from CBPs-treated rats improved dyslipidemia and reshaped gut microbiota in HFD-fed rats. In conclusion, CBPs treatment improved obesity and complications by regulating gut microbiota in HFD-fed rats. The gut microbiota plays an important role in BAs metabolism after CBPs treatment, and BAs have therefore emerged as major effectors in microbe-host signaling events that influence host lipid metabolism, energy metabolism and thermogenesis.
Validation of an e-health readiness assessment framework for developing countries
Authors: Mauco Kabelo Leonard , Scott Richard E , Mars Maurice
Published: June 2020

Background

Studies document e-health as having potential to improve quality of healthcare services, resulting in both developed and developing countries demonstrating continued interest in e-health uptake and use. e-Health implementations are not always successful as high failure rates have been reported in both developed and developing countries. These failures are often a result of lack of e-health readiness. e-Health readiness has been defined as the preparedness of healthcare institutions or communities for the anticipated change brought by programs related to information and communication technologies. As such it is critical to conduct an e-health readiness assessment prior to implementation of e-health innovations so as to reduce chances of project failure. Noting the absence of an adequate e-health readiness assessment framework (eHRAF) suitable for use in developing countries, the authors conceptualised, designed, and created a developing country specific eHRAF to aid in e-health policy planning. The aim of this study was to validate the developed eHRAF and to determine if it required further refinement before empirical testing.

Methods

Published options for a framework validation process were adopted, and fifteen globally located e-health experts engaged. Botswana experts were engaged using saturation sampling, while international experts were purposively selected. Responses were collated in an Excel spreadsheet, and NVivo 11 software used to aid thematic analysis of the open ended questions.

Results

Analysis of responses showed overall support for the content and format of the proposed eHRAF. Equivocal responses to some open ended questions were recorded, most of which suggested modifications to terms within the framework. One expert from the developed world had alternate views.

Conclusions

The proposed eHRAF provides guidance for e-health policy development and planning by identifying, in an evidence based manner, the major areas to be considered when preparing for an e-health readiness assessment in the context of developing countries.
SEIR model for COVID-19 dynamics incorporating the environment and social distancing
Authors: Mwalili Samuel , Kimathi Mark , Ojiambo Viona , Gathungu Duncan , Mbogo Rachel Waema
Published: June 2020

Objective: Coronavirus disease 2019 (COVID-19) is a pandemic respiratory illness spreading from person-to-person caused by a novel coronavirus and poses a serious public health risk. The goal of this study was to apply a modified susceptible-exposed-infectious-recovered (SEIR) compartmental mathematical model for prediction of COVID-19 epidemic dynamics incorporating pathogen in the environment and interventions. The next generation matrix approach was used to determine the basic reproduction number (O). The model equations are solved numerically using fourth and fifth order Runge Kutta methods.Results: We found an b of j, implying that the pandemic will persist in the human population absent strong control measures. Results after simulating various scenarios indicate that disregarding social distancing and hygiene measures can have devastating effects on the human population. The model shows that quarantine of contacts and isolation of cases can help halt the spread of novel coronavirus.
Head & neck acinar cell carcinoma: a population-based study using the seer registry
Authors: Yibulayin Feiluore , Feng Lei , Wang Meng , Lu Meng-meng , Luo Yuan , Liu Hui , Yang Zhi-cheng , Wushou Alimujiang
Published: June 2020

Background: to explore the clinicopathologic characteristics, treatment and prognostic factors of head and neck acinar cell carcinoma (HNACC) comprehensively. Methods: A population-based study was conducted using data from the Surveillance, Epidemiology, and End Results database (1975- 2016). Overall survival (OS) and HNACC-specific survival of patients with different clinicopathologic variables were compared using the Kaplan-Meier method and Cox multivariate regression. Results: A total of 2624 primary HNACC cases (1052 males, 1572 females) were identified. There was a significant difference in gender distribution. Among the total cohort, 2416 cases originated from salivary glands, including 2325 parotid gland ACC cases. Regardless of confounding factors, the 10-year and 20-year disease-specific survival (DSS) was 93.6% and 90%, respectively. Surgery was favourably associated with better DSS and OS [HR = 0.13, P = 0.0092 and HR = 0.23, P = 0.0203]. Gender was the only demographic independent prognostic factor for both DSS and OS [male vs female, HR = 3.3, P = 0.0028 for DSS; HR = 2.44, P = 0.0376 for OS]. Higher pathological grade was adversely associated with DSS and OS [Grade II, HR= 4.03, P = 0.0444; Grade III + IV, HR = 35.64, P = 0.0000 for DSS; Grade III + IV, HR = 4.49, P = 0.0000 for OS, Grade I as reference]. In addition, TNM/AJCC stage was commonly associated with prognosis. Conclusion: Surgery was the only favourable prognostic indicator for both DSS and OS. Gender, age, pathological differentiation and TNM/AJCC stage were independent prognostic factors for survival.
Spatiotemporal variability and trends in rainfall and temperature in Alwero watershed, western Ethiopia
Authors: ALEMAYEHU ARRAGAW , Maru Molla , Bewket Woldeamlak , Assen Mohammed
Published: June 2020

Background: Climate analysis at relevant time scales is important for water resources management, agricultural planning, flood risk assessment, ecological modeling and climate change adaptation. This study analyses spatiotemporal variability and trends in rainfall and temperature in Alwero watershed, western Ethiopia. The study is based on gridded monthly rainfall and maximum and minimum temperature data series at a resolution of 4x4 km which were obtained from the National Meteorological Agency of Ethiopia for the period 1983-2016. The study area is represented by 558 points (each point representing 4×4 km area). Results: Mean annual rainfall of the watershed is >1600mm. Annual, June-September (Kiremt), March-May (Belg) rainfall totals exhibit low inter-annual variability. Annual and October-February (Bega) rainfalls show statistically significant increasing trends at p=0.01 level. May and November rainfall show statistically significant increasing trends at p=0.01 level. March shows statistically significant decreasing trend at p=0.1 level. The mean annual temperature of the watershed is 25 0C with standard deviation of 0.31 0C and coefficient of variation of 0.01 0C. Mean annual minimum and maximum temperatures show statistically non-significant decreasing trends. Bega season experienced statistically significant deceasing trend in the maximum temperature at p = 0.01 level. The year-to-year variability in the mean annual minimum and maximum temperatures showed that the 2000s is cooler than the preceding decades. Unlike our expectations, annual and seasonal rainfall totals showed increasing trends while maximum and minimum temperatures showed decreasing trends. Conclusions: Our results suggest that local level investigations such as this one are important in developing context-specific climate change adaptation and agricultural planning, instead of coarse-scale national level analysis guiding local level decisions.
Combined subcostal and posterior transversus abdominis plane block for postoperative pain relief after abdominoplasty
Authors: Sayed Haytham El , Fadhul A Shaheed , Falalki Mohamed Al , Awad M Naser Naser
Published: June 2020

Background
Abdominoplasty is a common aesthetic surgery. Adequate pain management during the postoperative period is of great importance. Previous studies have failed to achieve safe and reliable effective analgesic techniques beyond the recovery room. This research aims to investigate the outcome of the abdominoplasty operation for the patients' received transversus abdominis plan block in comparison with the non-blocked patients.
Methods
58 patients, undergoing elective abdominoplasty, received general anesthesia. Patients were randomly assigned to two equal groups of 29 patients each. Combined subcostal and posterior transversus abdominis plane block group and non-blocked group. For both groups, the standard postoperative analgesic regimen consisted of IV Paracetamol 1 g every 6 hours. Values of visual analog scale values were recorded every 4 hours postoperatively, once the patient had a visual analog scale ≥ 6, IV narcotics administered and visual analog scale recorded every 30 min till pain improved.
Results
there’s a significant difference between both groups regarding the visual analog scale data, patient ambulation, patients’ need for postoperative mechanical ventilatory support, and also the dosage of narcotics used.
Conclusion
Transversus abdominis plane block is a promising regional anesthetic technique for postoperative pain relief in abdominoplasty surgery, it offers a longer postoperative analgesic effect duration and fewer analgesic requirements with less postoperative complications.
A preliminary study on the joint parameter Lym% and HGB for the prediction of severe and nonsevere COVID-19
Authors: Zhang Wenping , Zhang Zhongming , Ye Yi , Luo Yanting , Pan Shiyao , Qi Huan , Yu Zhiyong , Qu Jiuxin
Published: June 2020

Objectives

The aim of this study was to identify the parameters in routine blood tests that can be used to evaluate the severity of coronavirus disease 2019 (COVID-19) and thus assist in clinically predicting the extent of progression.
Methods

This study retrospectively analyzed the epidemiological, clinical symptom and laboratory examination data of 159 COVID-19 patients. The percentage of lymphocytes (Lym%) and hemoglobin (HGB) were integrated into a joint parameter, Lym%&HGB, by binary logistic regression.
Results

Both Lym% and HGB gradually decreased with disease progression whereas the joint parameter Lym%&HGB increased gradually with disease progression. When using Lym%, HGB, and Lym%&HGB to predict COVID-19 severity, the area under the receiver operating characteristic (ROC) curve (AUC) was 0.89, 0.79, and 0.92, respectively. The dynamic change curves showed that Lym% and HGB continued to decline while Lym%&HGB continued to increase with disease progression in patients with severe COVID. The change in Lym%&HGB was more prominent than the changes in Lym% and HBG.
Conclusions

The joint parameter Lym%&HGB can serve as a good tool to differentiate severe and nonsevere COVID-19, and it has a higher sensitivity and specificity than either Lym% or HGB alone.
Immunological Analysis and Differential Genes Screening of Venous Thromboembolism
Authors: Gao Li-Na , Li Qiang , Xie Jian-Qin , Yang Wan-Xia , You Chong-Ge
Published: June 2020

Purpose: To explore the pathogenesis of venous thromboembolism (VTE) and provide bioinformatics basis for the prevention and treatment of VTE. Methods: The R software was used to obtain the gene expression profile data of GSE19151, combining with the CIBERSORT database, obtain immune cells and differentially expressed genes (DEGs) of blood samples of VTE patients and normal control, and analyze DEGs for GO analysis and KEGG pathway enrichment analysis. Then, the protein-protein interaction (PPI) network was constructed by using the STRING database, the key genes (hub genes) and immune differential genes were screened by Cytoscape software, and the transcription factors (TFs) regulating hub genes and immune differential genes were analyzed by the NetworkAnalyst database. Results: Compared with the normal group, monocytes and resting mast cells were significantly expressed in the VTE group, while regulatory T cells were significantly lower. Ribosomes were closely related to the occurrence of VTE. 10 hub genes and immune differential genes were highly expressed in VTE. MYC, SOX2, XRN2, E2F1, SPI1, CREM and CREB1 can regulate the expressions of hub genes and immune differential genes. Conclusions: Ribosomal protein family genes are most relevant to the occurrence and development of VTE, and the immune differential genes may be the key molecules of VTE, which provides new ideas for further explore the pathogenesis of VTE.
Iron Overload Adversely Effects Bone Marrow Haematogenesis via SIRT-SOD2-MROS in a Process Ameliorated by Curcumin
Authors: zhou shujuan , Sun Lan , Qian Shanhu , Ma Yongyong , Ma Ruye , Dong Yuqing , Shi Yifen , Jiang Songfu , Ye Haige , Shen Zhijian , Zhang Shenghui , Shen Jianping , Yu Kang , wang siqian
Published: June 2020

Background: Iron overload is common in patients with haematological disorders, and it is known to have a suppressive effect on haematogenesis. However, the mechanism by which iron overload affects haematogenesis is still unclear. The antioxidant curcumin has been reported to protect against iron overload-induced bone marrow damage, although the mechanism underlying this protective effect remains to be elucidated.Methods: We established iron overload cell and mouse models. Mitochondrion-derived reactive oxygen species (mROS) levels, autophagy levels, and the SIRT3/SOD2 pathway were examined in these models and in the bone marrow of patients with iron overload.Results: Iron overload was shown to depress haematogenesis and induce mitochondrion-derived superoxide anion-dependent autophagic cell death. Iron loading decreased SIRT3 protein expression, promoted an increase in SOD2, and led to the elevation of mROS. These effects were reversed by the overexpression of SIRT3. Curcumin treatment ameliorated peripheral blood cells, enhanced SIRT3 activity, decreased SOD2 acetylation, inhibited mROS production, and suppressed iron loading-induced autophagy.Conclusions: These results suggest that curcumin exerts a protective effect on bone marrow by reducing mROS-stimulated autophagic cell death in a manner dependent on the SIRT3/SOD2 pathway.
Improving Mental Health Care for Unaccompanied Young Refugees through a Stepped Care Approach versus Usual Care+: Study Protocol of a Cluster Randomized Controlled Hybrid Effectiveness Implementation Trial
Authors: Rosner Rita , Sachser Cedric , Hornfeck Fabienne , Kilian Reinhold , Kindler Heinz , Muche Rainer , Müller Lauritz Rudolf Floribert , Thielemann Jonathan , Waldmann Tamara , Ziegenhain Ute , Unterhitzenberger Johanna , Pfeiffer Elisa
Published: June 2020

Background: More than half of the unaccompanied young refugees (UYRs) resettled in Europe report elevated levels of posttraumatic stress symptoms (PTSS) and comorbid symptoms. Earlier studies have highlighted the effectiveness of the trauma-focused preventive group intervention ‘Mein Weg’ (English ‘My Way’), and the feasibility of trauma-focused cognitive behavioral therapy (TF-CBT) for UYRs. Both interventions are deemed to be empirically supported treatments (ESTs). However, UYRs seldom receive ESTs or, in fact, any treatment at all. In view of the high need and the limited treatment resources available, a stepped-care approach is indicated but has not been evaluated so far. The purpose of this trial is to compare the stepped-care approach BETTER CARE with usual care enhanced with screening and indication (usual care+).Methods: In a cluster randomized controlled trial involving N = 540 UYRs living in up to N=54 child and youth welfare service (CYWS) facilities, BETTER CARE will be compared with usual care+. We will randomize clusters comprising a CYWS facility with at least one eligible psychotherapist. BETTER CARE consists of step 1) screening and indication and either step 2) preventive trauma-focused group intervention ‘Mein Weg’ delivered by trained CYWS staff or step 3) TF-CBT delivered by trained community therapists and supported by trained translators if necessary. Participants will be assessed 6 and 12 months after randomization. The primary outcome is the severity of PTSS after 12 months. Secondary outcomes are depressive and anxiety symptoms, quality of life, and proxy reported PTSS. Furthermore, drug use, health costs, benefits and long-term effects on integration/acculturation will be assessed. Discussion: The trial will directly integrate a stepped-care approach into existing structures of the German child welfare and (mental) health system. It could, therefore, serve as a blueprint for how to implement ESTs for UYRs. If successful, screening, prevention and intervention will be sustainably implemented in CYWS in southern Germany.Trial registration: German Clinical Trials Register, ID: DRKS00017453. Registered December 11th, 2019. URL: https://www.drks.de/drks_web/setLocale_EN.do
LBX2-AS1 Promotes Ovarian Cancer Progression by Facilitating E2F2 Gene Expression via MIR-455-5P and MIR-491-5P Sponging
Authors: Cao Jian , Wang Huan , Tang Ranran , Liu Guangquan , Xu Pengfei , Wang Huayu , Miao Juan , Gu Xiaoyan , Han Suping
Published: June 2020

Background:LBX2-AS1 is a long noncoding RNA that facilitates the development of gastrointestinal cancers and lung cancer, but its participation in ovarian cancer development remained uninvestigated.Methods: Clinical data retrieved from TCGA ovarian cancer database and the clinography of 60 ovarian cancer patients who received anti-cancer treatment in our facility were analyzed. The overall cell growth, colony formation, migration, invasion, apoptosis and tumor formation on nude mice of ovarian cancer cells were evaluated before and after lentiviral-based LBX2-AS1 knockdown. ENCORI platform was used to explore LBX2-AS1-interacting microRNAs and target genes of the candidate microRNAs. Luciferase reporter gene assay and RNA-pulldown assay were used to verify the putative miRNA-RNA interactions.Results: Ovarian cancer tissue specimens showed significant higher LBX2-AS1 expression levels that non-cancerous counterparts. High expression level of LBX2-AS1 significantly associated with patients reduced overall survival. LBX2-AS1 knockdown significantly downregulated the cell growth, colony formation, migration, invasion and tumor formation capacity of ovarian cancer cells and increased their apoptosis in vitro. LBX2-AS1 interacts with and thus inhibits the function of miR-455-5p and miR-491-5p, both of which restrained the expression of E2F2 gene in ovarian cancer cells via mRNA targeting. Transfection of miRNA inhibitors of these two miRNAs or forced expression of E2F2 counteracted the effect of LBX2-AS1 knockdown on ovarian cancer cells.ConclusionsLBX2-AS1 was a novel cancer-promoting lncRNA in ovarian cancer. This lncRNA increased the cell growth, survival, migration, invasion and tumor formation of ovarian cancer cells by inhibiting miR-455-5p and miR-491-5p, thus liberating the expression of E2F2 cancer-promoting gene.
Clinical characterisation and the improved molecular diagnosis of autosomal dominant cone-rod dystrophy in patients with SCA7
Authors: Zou Xuan , Yao Fengxia , Li Fengrong , Wu Shijing , Li Hui , Sun Zixi , Zhu Tian , Wei Xing , Li Donghui , Sui Ruifang
Published: June 2020

Purpose

To evaluate the retinal phenotype and genetic features of Chinese patients with spinocerebellar ataxia type 7 (SCA7).
Methods

Detailed ophthalmic examinations, including electroretinograms, fundus photography, fundus autofluorescence and optical coherence tomography, were performed to analyse the retinal lesions of patients with SCA7. A molecular genetic analysis was completed to confirm the number of CAG repeats in ATXN7 gene on the patients and their family members.
Results

Eight patients from three families with SCA7 were included in this study. Trinucleotide repeat was expanded from 43 to 113 in the affected patients. The affected patients were characterised by different degrees of cone-rod dystrophy, which is positively related to the number of CAG repeats and age. All patients complained of progressive bilateral visual loss, and most cases reported visual disturbance earlier than gait movement or dysarthria. A coarse granular appearance of the macular region on scanning laser ophthalmoscopy, hypofluorescence in the macula on autofluorescence, retinal atrophy on optic coherence tomography, depression of multifocal electroretinograms and prominent abnormalities in cone-mediated responses on electrograms are the general features of SCA7-related retinopathy. Hyperreflective dots in the outer retinal layers and choroidal vessel layers are a common sign in optic coherence tomography in the advanced stage.
Conclusions

SCA shows a cone-rod dystrophy phenotype. The multimodal imaging of the retina is beneficial to detect the early lesions of cone-rod dystrophy related to SCA7.
Regeneration of caudate lobe after living donor liver transplantation: Comparison with a surrogate model of left lobe graft
Authors: Saito Yu , Imura Satoru , Morine Yuji , Ikemoto Tetsuya , Yamada Shinichiro , Shimada Mitsuo
Published: June 2020

Background:
The aim of this study is to clarify the regeneration of the CL (caudate lobe) without any reconstructions of short hepatic veins (SHVr) after LDLT (living donor liver transplantation) and compare the regeneration of the CL after right hepatectomy (Rt. Hx), as the surrogate model of extended left lobe graft (Ex LLG) with complete SHVr.
Methods:
Eleven Ex LLGs with CL were included in this study. SHVr was not performed in all cases. The volumetry was performed before, one month and six months after LDLT. Seven patients who underwent Rt. Hx were also included in this study as the surrogate model.
Results:
In Ex LLGs with CL, the regeneration rate of the large CL (> 30 ml) was worse than that of small CL (< 30 ml). In the surrogate model, the regeneration rate of the CL was not worse than other segments. However, the regeneration rate of the large CL was also worse than that of small CL even in the presence of complete SHVr.
Conclusions:
The regeneration of the large CL was worse than that of the small CL regardless of the presence or absence of SHVr, indicating that SHVr in Ex LLG with CL might not be necessary.