bluuebunny/crossref_metadata_2025 huggingface dataset:
Citric Acid Production by Yarrowia lipolytica NRRL Y-1094: Optimization of pH, Fermentation Time and Glucose Concentration Using Response Surface Methodology
Authors: Sayın Börekçi Bilge , Kaya Mükerrem , Kaban Güzin
Published: December 2022

In this study, three Yarrowia lipolytica strains (Y. lipolytica NRRL Y-1094, Y. lipolytica NRRL YB-423 and Y. lipolytica IFP29) were screened for acid-production capacity and the maximum zone-area was formed by Y. lipolytica NRRL Y-1094. The strain was then selected as a potential citric-acid (CA) producer for further studies. The CA production by Y. lipolytica NRRL Y-1094 was optimized using response surface methodology (RSM) and considering three factors, comprising initial pH-value, fermentation time, and initial glucose-concentration. The highest CA-concentration was 30.31 g/L under optimum conditions (pH 5.5, 6 days, and 125 g/L glucose) in shake flasks. It has been reported that this result gives better results than many productions with shake flasks. According to estimated regression-coefficients for CA concentration, the fermentation time had the greatest impact on CA production, followed by the substrate concentration and initial pH-level, respectively. On the other hand, this study is a fundamental step in solving and optimizing the production mechanism of Y. lipolytica NRRL Y-1094, a microorganism that has not yet been used in CA production with a glucose-based medium. The results suggest that future studies can perform higher yields by optimizing other medium constituents and environmental factors.
Gamification with Scratch or App Inventor in Higher Education: A Systematic Review
Authors: Pérez-Jorge David , Martínez-Murciano María Carmen
Published: December 2022

Programming skills should be taught and developed; Scratch and App Inventor are two tools that can contribute significantly to developing this competence in university students. This study aims to investigate the use and effect of the programming language Scratch and App Inventor on the development of skills and competencies for learning (autonomy, attention, motivation, critical thinking, creative thinking, computational thinking, communication, problem solving and social interaction) in higher education. To achieve this goal, a systematic review of articles in English and Spanish was carried out using the PRISMA statement (research publication guidelines designed to improve the integrity of systematic review and meta-analysis reports). A search for studies was conducted in the Web of Science (WOS), Dialnet, and SCOPUS. A total of 405 papers were analyzed, of which 11 were finally selected. The results showed that both Scratch and App Inventor favor the development of skills and competencies for learning in the context of higher education, despite being underutilized strategies that all knowledge disciplines should promote.
Effects of Concentration of Soybean Protein Isolate and Maltose and Oil Phase Volume Fraction on Freeze–Thaw Stability of Pickering Emulsion
Authors: Song Ziyue , Yang Yang , Chen Fenglian , Fan Jing , Wang Bing , Bian Xin , Xu Yue , Liu Baoxiang , Fu Yao , Shi Yanguo , Zhang Xiumin , Zhang Na
Published: December 2022

There is growing interest in enhancing the freeze–thaw stability of a Pickering emulsion to obtain a better taste in the frozen food field. A Pickering emulsion was prepared using a two-step homogenization method with soybean protein and maltose as raw materials. The outcomes showed that the freeze–thaw stability of the Pickering emulsion increased when prepared with an increase in soybean protein isolate (SPI) and maltose concentration. After three freeze–thaw treatments at 35 mg/mL, the Turbiscan Stability Index (TSI) value of the emulsion was the lowest. At this concentration, the surface hydrophobicity (H0) of the composite particles was 33.6 and the interfacial tension was 44.34 mN/m. Furthermore, the rheological nature of the emulsions proved that the apparent viscosity and viscoelasticity of Pickering emulsions grew with a growing oil phase volume fraction and concentration. The maximum value was reached in the case of the oil phase volume fraction of 50% at a concentration of 35 mg/mL, the apparent viscosity was 18 Pa·s, the storage modulus of the emulsion was 575 Pa, and the loss modulus was 152 Pa. This research is significant for the production of freeze–thaw resistant products, and improvement of protein-stabilized emulsion products with high freeze–thaw stability.
The Impact of Brand Awareness and Country of Origin in the Advertising Effectiveness of Greek Food Products in the United Kingdom: The Case of Greek Yogurt
Authors: Karagiannis Dimitrios , Hatzithomas Leonidas , Fotiadis Thomas , Gasteratos Antonios
Published: December 2022

The present study aims to investigate the country-of-origin effect and the branding process on the brand equity of Greek yogurt abroad, specifically in the United Kingdom. The research was carried out as a 2 × 2 experiment using a closed-ended questionnaire with the participation of a total of 400 consumers, using two Greek yogurts (branded and non-branded) as the product under study, with or without country-of-origin insignia (viz. the Greek flag) on the packaging. According to the research results, brand equity was found to be higher for the branded Greek yogurt with country-of-origin insignia among the four product categories, as reflected in its three sub-dimensions (brand awareness, loyalty, and perceived quality). It was also found that country of origin influences brand equity regardless of branding, a finding that confirms the significant effect of this factor on consumers’ perceptions. This highlights the distinction between perceived quality and the specific image of the country in terms of its production experience for a specific product category. Based on these findings, Greek yogurt companies exporting abroad should lay great emphasis on developing branding strategies to strengthen their product brand, while at the same time incorporate country of origin as an indicator of the quality of their brand. The latter finding applies in particular to less well-known brands, since geographical indication (insignia) or other strategies that promote the country of origin are perceived as important informational cues for consumers.
Assessment of Potential Probiotic and Synbiotic Properties of Lactic Acid Bacteria Grown In Vitro with Starch-Based Soluble Corn Fiber or Inulin
Authors: García-Núñez Iris M. , Santacruz Arlette , Serna-Saldívar Sergio O. , Hernandez Sandra L. Castillo , Amaya Guerra Carlos A.
Published: December 2022

This research is aimed to search for suitable probiotic plus prebiotic combinations for food applications. Sixteen bacteria were tested for resistance to low pH, bile salts and antibiotics, and their adhesion to Caco-2 cells, in order to select potential probiotics. Then, two bacteria were selected to study short chain fatty acids production in a starch-based soluble corn fiber or inulin media. Lactiplantibacillus plantarum V3 and L. acidophilus La3 manifested the best probiotic features with a remarkable adhesion ability (23.9% and 17.3%, respectively). Structural differences between fibers have an impact on how each one is metabolized, both in their capacity of being easily fermented and in the short chain fatty acids profile obtained: L. acidophilus La3 in inulin fermentation yielded the highest total short chain fatty acids (85.7 mMol/L), and, in starch-based soluble corn fiber fermentation, yielded the highest butyric acid content (0.31 mMol/L). This study provides valuable information for future design of synbiotics for food applications.
Conditions of the Stepwise Cooling Algorithm for Stable Supercooling Preservation and Freshness of Pork Loin
Authors: Park Dong , Kim Eun , Kim Honggyun , Hong Geun-Pyo , Choi Mi-Jung
Published: December 2022

Supercooling has the advantage of maintaining the freshness of foods without a phase transition. However, it is hard to sustain the supercooled state. Static temperature control, one of the various supercooling technologies, is used for stable supercooling storage. In this experiment, the effect of following external factors in maintaining the supercooled state of foods was investigated. Three main parameters had an effect on the supercooled state of food: (1) properly setting the lower-temperature limit of the supercooling algorithm, (2) slow cooling to the target temperature, and (3) minimizing temperature fluctuation. Accordingly, the following stepwise cooling algorithm for pork loin was designed: a lower-temperature limit of −3.0 °C and a storage period = 36 h followed by a lower-temperature limit of −3.5 °C for 24 h. The samples conserved at −3.0 °C displayed a 100% supercooled state. Physicochemical properties including drip loss, cooking loss, texture, color, total volatile basic nitrogen (TVBN), and total aerobic count (TAC) of pork loin were analyzed. The drip loss values of the supercooled meat samples were lower than those of the superchilled ones. Furthermore, TVBN and TAC of the treated samples were not significantly different from those of the fresh samples (p > 0.05). In conclusion, supercooling storage extended the freshness and quality of pork loin better than refrigerated storage.
“They Kept Going for Answers”: Knowledge, Capacity, and Environmental Health Literacy in Michigan’s PBB Contamination
Authors: Lebow-Skelley Erin , Fremion Brittany B. , Quinn Martha , Makled Melissa , Keon Norman B. , Jelenek Jane , Crowley Jane-Ann , Pearson Melanie A. , Schulz Amy J.
Published: December 2022

The Michigan PBB Oral History Project documented community residents’ descriptions of a large-scale chemical contamination—the PBB contamination—that occurred in Michigan in 1973. These oral histories document residents’ and others’ experiences during and after the contamination. We conducted a grounded theory qualitative analysis of 31 oral histories to examine the experiences of community members, researchers, lawyers, and others who actively sought out and contributed essential information about the contamination and its impacts. Our findings highlight several challenges encountered in the development of this knowledge including four central themes—contested knowledge, community skills, inaction, and uncertainty. Integrating environmental health literacy, community capacity, and contested illness frameworks, we examine the contributions of community residents, scientists (from inside and outside the community), and others to the development of knowledge to inform decisions and sustain action regarding this large-scale contamination. We close with a discussion of lessons learned regarding efforts to build environmental health knowledge within uncertain and often contested contexts and for promoting environmental health and action related to large-scale chemical contaminations. Our findings suggest the importance of integrated frameworks for examining and promoting the critical role of community skills, leadership, participation, sense of community, and community power in promoting environmental health.
Diagnostic Study to Assess the Performance of a New Urinary Legionella Antigen Test—A National Study in Three Referral University Hospitals in Austria during 2014–2017
Authors: El-Khatib Ziad , Richter Lukas , Ressler Jutta , Benka Bernhard
Published: December 2022

Background: We evaluated the performance of a rapid diagnostic antigen test (Coris) as an index test versus the urinary Antigen ELISA (Bartels) as the reference test. Methods: Prospective diagnostic accuracy study (2014–2017) at three university hospitals in Austria. Results: A total of 996 patients were included in the study. Legionellosis was diagnosed in 49/996 (4.9%) using the reference test. The sensitivity and specificity of the Coris test were 75.5% (95% CI 61.1–86.7%) and 100% (95%CI 99.6–100%), respectively. The PPV was 100% and when using the lower 95% CI limit of the estimate for sensitivity, the resulting PPV was 61.1%. The NPV was 98.7% and the accuracy was 98.8%. The index test showed a PPV > 97% during the period of summer and autumn (May through November) and ≥88% during winter (December through February). The NPV was >97% during all of the periods. The median of the monthly incidence in the general population was 0.1 per 100,000 (IQR 0.1; 0.3). Conclusion: The new rapid test gave a high level of diagnostic accuracy in a rapid fashion. The test can be applied at the bedside by non-laboratory staff.
Ratings of Hand Activity and Force Levels among Women and Men Who Perform Identical Hand-Intensive Work Tasks
Authors: Dahlgren Gunilla , Liv Per , Öhberg Fredrik , Slunga Järvholm Lisbeth , Forsman Mikael , Rehn Börje
Published: December 2022

We compared hand activity and force ratings in women and men doing identical hand-intensive work tasks. Musculoskeletal disorders are more common in women and hand-intensive work leads to an increased risk of these disorders. Knowledge of the gender influence in the rating of work exposure is lacking. The aim of this study was to investigate whether women and men performing identical hand-intensive work tasks were equally rated using hand activity and normalized peak force levels with the Hand Activity Threshold Limit Value®. Fifty-six workers participated, comprising 28 women–men pairs. Four observers—two woman–man pairs—were also involved. Self-ratings and observers’ ratings of hand activity and force level were collected. The results of these ratings showed no significant gender differences in self-rated hand activity and force, as well as observer-rated hand activity. However, there was a significant gender difference in the observer-rated force, where the women were rated higher (mean (SD): women 3.9 (2.7), men 3.1 (1.8) (p = 0.01)). This difference remained significant in the adjusted model (p = 0.04) with grip strength and forearm–finger anthropometrics. The results provide new insights that observers’ estimates of force can be higher in women compared with men in the same work tasks. Force should be further investigated and preferably compared to objective measurements.
The Role of Organizational Factors in Nurses’ Perceived Preparedness to Screen, Intervene and Refer in Cases of Suspected Postpartum Depression
Authors: Bina Rena , Glasser Saralee , Honovich Mira , Ferber Yona , Alfayumi-Zeadna Samira
Published: December 2022

Routine screening for postpartum depression (PPD) is widespread, yet little attention has been given to the perceived preparedness of health providers to perform screening procedures, or to the role of organizational factors in their preparedness, although these are crucial elements for optimal implementation. The aim of this study was to examine organizational factors associated with public health nurses’ (PHNs) perceived preparedness to screen women for PPD, intervene, and refer them in cases of suspected PPD. Two hundred and nineteen PHNs completed a self-report survey regarding their perceived preparedness to carry out a screening program (including screening, intervening, and referring women), and their perceived organizational support, supervisor’s support, colleagues’ support, and colleagues’ preparedness. A path analysis model was used to analyze the data. The results showed that perceived colleagues’ preparedness was significantly associated with the three perceived preparedness constructs (screen, intervene, and refer). Perceived supervisor’s support was positively associated with perceived preparedness to screen, and perceived organizational support was positively associated with perceived preparedness to intervene. This paper highlights the manner in which formal and informal organizational factors play an important role in the perceived preparedness of PHNs to carry out a PPD screening program, and how these factors impact the three different aspects of the screening program. Organizations implementing PPD screening should support PHNs in all aspects of the implementation process, provide guidance, and enhance peer-group continued learning through which PHNs could share knowledge, discuss barriers, and foster professional development.
Evaluation and Strategic Response of Sustainable Livelihood Level of Farmers in Ecological Resettlement Area of the Upper Yellow River—A Case Study of Liujiaxia Reservoir Area, Gansu Province
Authors: Wang Zongxiang , Li Wei , Qi Jianwu
Published: December 2022

The level of sustainable livelihoods, as a yardstick for measuring the social development of migrants, is of great importance to the sustainable development of the region. Based on the analysis of the policy logic of ecological protection and high-quality development in the Yellow River basin, this paper constructs a “ternary” system model and evaluation index system for sustainable livelihoods of farm households in the ecological resettlement areas of the upper Yellow River, and proposes that the harmonious relationship between the three basic dimensions of economy, society and environment is the key to evaluate the sustainable livelihood level of farm households in ecological resettlement areas. Based on the comprehensive evaluation index to assess the comprehensive development level of ecological resettlement areas, we introduced the coupling coordination degree and constructed the coordinated development degree model of “economic-social-environmental” system to characterize the sustainable livelihood level. Through the data of 1116 questionnaires and in-depth interviews in the ecological migrant resettlement area of Liujiaxia reservoir in the upper reaches of the Yellow River basin, the sustainable livelihood status and spatial distribution differences of farm households in 14 townships in the region were evaluated, and the validity of the indicator system was empirically tested. Finally, sustainable livelihood strategies for farm households in the ecological resettlement areas of the upper Yellow River are proposed for the economic, social and environmental dimensions, and the indicator system is further revised. The evaluation system can not only advance the research paradigm of sustainable livelihood assessment for farmers in ecological migrant resettlement areas but can also be widely guided and applied to the sustainable development of ecological migrant practices in China.
Nanocellulose-Based Polymer Composites Functionalized with New Gemini Ionic Liquids
Authors: Zielińska Daria , Skrzypczak Andrzej , Peplińska Barbara , Borysiak Sławomir
Published: December 2022

The manuscript discusses the application of dimeric imidazolium ionic liquids with an aliphatic linker of different lengths, constituting a new class of compounds called gemini, for the modification of renewable materials. This innovative functionalization with the use of ionic liquids made it possible to obtain polymer composite nanomaterials with renewable fillers, which will reduce the consumption of petroleum-based raw materials and also be directly related to the reduction of energy intensity. Renewable filler in the form of nanocellulose modified with ionic liquids, as well as polymer composites with such filler obtained by extrusion and injection molding techniques, were subjected to detailed characterization using techniques like: X-ray diffraction (XRD), Fourier transform spectroscopy (FTIR), dispersion studies (DLS), morphological analysis (SEM), differential scanning calorimetry (DSC), hot-stage polarized light microscopy and characterization of mechanical properties. The use of innovative dimeric ionic liquids proved to be an effective method to carry out efficient functionalization of cellulose. This provided a stable space structure between polysaccharide particles, limiting aggregate formation. It was shown that chemical modification with ionic liquids has a significant effect on the nucleation activity of cellulose fillers and the formation of the supermolecular structure of the polymer matrix, which consequently allowed to obtain polymer composites with excellent strength characteristics and increased flexibility, which will allow to increase their application potential. Innovative ionic liquids have contributed to obtaining green nanomaterials with excellent functional properties, which have not been described in the literature so far.
SWAR: A Deep Multi-Model Ensemble Forecast Method with Spatial Grid and 2-D Time Structure Adaptability for Sea Level Pressure
Authors: Zhang Jingyun , Xu Lingyu , Jin Baogang
Published: December 2022

The multi-model ensemble (MME) forecast for meteorological elements has been proved many times to be more skillful than the single model. It improves the forecast quality by integrating multiple sets of numerical forecast results with different spatial-temporal characteristics. Currently, the main numerical forecast results present a grid structure formed by longitude and latitude lines in space and a special two-dimensional time structure in time, namely the initial time and the lead time, compared with the traditional one-dimensional time. These characteristics mean that many MME methods have limitations in further improving forecast quality. Focusing on this problem, we propose a deep MME forecast method that suits the special structure. At spatial level, our model uses window self-attention and shifted window attention to aggregate information. At temporal level, we propose a recurrent like neural network with rolling structure (Roll-RLNN) which is more suitable for two-dimensional time structure that widely exists in the institutions of numerical weather prediction (NWP) with running service. In this paper, we test the MME forecast for sea level pressure as the forecast characteristics of the essential meteorological element vary clearly across institutions, and the results show that our model structure is effective and can make significant forecast improvements.
Relationships between the Nutrition Status and Oral Measurements for Sarcopenia in Older Japanese Adults
Authors: Okuno Kentaro , Kobuchi Ryuichiro , Morita Suguru , Masago Ayako , Imaoka Masaaki , Takahashi Kazuya
Published: December 2022

Introduction: The purpose of the present study was to clarify the relationships between the risk of malnutrition as a preliminary stage of malnutrition and overall and oral measurements for sarcopenia in older Japanese adults. Methods: Forty-five participants (79.7 ± 6.1 years) were included in the analysis. The nutrition status of the participants was assessed using the Mini Nutritional Assessment-Short Form (MNA-SF) and classified into two groups: normal and at risk of malnutrition. Overall measurements for sarcopenia in the present study were the skeletal muscle mass index, grip strength, and walking speed, while oral measurements were the cross-sectional area of the geniohyoid muscle, tongue pressure (TP), and oral diadochokinesis. Results: MNA-SF correlated with TP (r = 0.347, p = 0.019). We observed decreases of 5.7 kPa in TP and 3.9 kg/cm2 in BMI in the at risk of malnutrition group. A multiple regression analysis of parameters contributing to the risk of malnutrition identified TP as an independent variable (β = 0.913, p = 0.042). Conclusions: The present results demonstrate that the risk of malnutrition is associated with TP as an oral measurement for sarcopenia, but not overall measurements for sarcopenia. Therefore, low TP may be related with the risk of malnutrition.
An Impairment in Resting and Exertional Breathing Pattern May Occur in Long-COVID Patients with Normal Spirometry and Unexplained Dyspnoea
Authors: Frizzelli Annalisa , Di Spigno Francesco , Moderato Luca , Halasz Geza , Aiello Marina , Tzani Panagiota , Manari Gaia , Calzetta Luigino , Pisi Roberta , Pelà Giovanna , Piepoli Massimo , Chetta Alfredo
Published: December 2022

Background: Long-term sequelae, called Long-COVID (LC), may occur after SARS-CoV-2 infection, with unexplained dyspnoea as the most common symptom. The breathing pattern (BP) analysis, by means of the ratio of the inspiratory time (TI) during the tidal volume (VT) to the total breath duration (TI/TTOT) and by the VT/TI ratio, could further elucidate the underlying mechanisms of the unexplained dyspnoea in LC patients. Therefore, we analysed TI/TTOT and VT/TI at rest and during maximal exercise in LC patients with unexplained dyspnoea, compared to a control group. Methods: In this cross-sectional study, we enrolled LC patients with normal spirometry, who were required to perform a cardio-pulmonary exercise test (CPET) for unexplained dyspnoea, lasting at least 3 months after SARS-CoV-2 infection. As a control group, we recruited healthy age and sex-matched subjects (HS). All subjects performed spirometry and CPET, according to standardized procedures. Results: We found that 42 LC patients (23 females) had lower maximal exercise capacity, both in terms of maximal O2 uptake (VO2peak) and workload, compared to 40 HS (22 females) (p < 0.05). LC patients also showed significantly higher values of TI/TTOT at rest and at peak, and lower values in VT/TI at peak (p < 0.05). In LC patients, values of TI/TTOT at peak were significantly related to ∆PETCO2, i.e., the end-tidal pressure of CO2 at peak minus the one at rest (p < 0.05). When LC patients were categorized by the TI/TTOT 0.38 cut-off value, patients with TI/TTOT > 0.38 showed lower values in VO2peak and maximal workload, and greater values in the ventilation/CO2 linear relationship slope than patients with TI/TTOT ≤ 0.38 (p < 0.05). Conclusions: Our findings show that LC patients with unexplained dyspnoea have resting and exertional BP more prone to diaphragmatic fatigue, and less effective than controls. Pulmonary rehabilitation might be useful to revert this unpleasant condition.
An Improved Ship Weather Routing Framework for CII Reduction Accounting for Wind-Assisted Rotors
Authors: Sun Wenyu , Tang Siyu , Liu Xiyang , Zhou Shinan , Wei Jinfang
Published: December 2022

With the increasingly strict regulations for the energy-saving and emission-reduction technology of ships, minimizing fuel cost and thus reducing the carbon intensity index (CII) is one of the most critical issues in the design and operation of merchant ships. More recently, many wind-assisted devices, such as rotors, wind sails, etc., have been investigated and designed to utilize renewable wind energy. With the equipment of wind-assisted rotors, the optimization of ship routes becomes more important because the effect of this wind-assisted device highly depends on the local wind field along the shipping route. In this paper, an improved ship weather routing framework based on the A* algorithm has been proposed to determine the optimal ship route and ship operations with wind-assisted rotors. The proposed framework effectively utilizes different sources of data, including ship design, weather forecasting and historical sailing information, to produce a better estimation of fuel consumption under the effect of sea states. Several improvements on the classic A* algorithm, including directed searching and three-dimensional extension, are proposed to improve the routing effect and efficiency. Finally, the proposed method was applied to test cases of a VLCC operating from China to the Middle East and the results show that the total fuel consumption could be reduced compared to the minimum distance route.
Compensation of Optical Pump Magnetometer Using the Improved Mayfly Optimization Algorithm
Authors: Li Linfeng , Liu Weidong , Li Le , Jiao Huifeng , Qu Junqi , Sun Gongwu
Published: December 2022

In order to solve the problem that the cesium optical pump magnetometer is disturbed by the carrier’s interference magnetic field during magnetic field anomaly detection, an interference magnetic field compensation method based on an improved mayfly optimization algorithm (IMOA) was proposed in this paper. First, by combining the measurement results of the attitude sensor with the geomagnetic inclination and magnetic declination in the locality, the measurement results of the optical pump magnetometer can be decomposed into the component values under the three axes of the carrier coordinate system. A compensation model including the carrier interference magnetic field was established. Then, considering the poor global search performance that existed in the mayfly optimization algorithm (MOA), an elite chaotic reverse learning strategy and Levy mutation strategy were introduced to improve the MOA. The compensation performance of the IMOA was estimated with a series of field experiments and compared with the stretching particle swarm optimization algorithm. The experiment results indicated that these two methods can effectively compensate the magnetometer’s measurement values, and that the IMOA method more easily jumps out of the local optimum, and has higher compensation accuracy.
β-Xylosidase SRBX1 Activity from Sporisorium reilianum and Its Synergism with Xylanase SRXL1 in Xylose Release from Corn Hemicellulose
Authors: Mercado-Flores Yuridia , Téllez-Jurado Alejandro , Lopéz-Gil Carlos Iván , Anducho-Reyes Miguel Angel
Published: December 2022

Sposisorium reilianum is the causal agent of corn ear smut disease. Eleven genes have been identified in its genome that code for enzymes that could constitute its hemicellulosic system, three of which have been associated with two Endo-β-1,4-xylanases and one with α-L-arabinofuranosidase activity. In this study, the native protein extracellular with β-xylosidase activity, called SRBX1, produced by this basidiomycete was analyzed by performing production kinetics and its subsequent purification by gel filtration. The enzyme was characterized biochemically and sequenced. Finally, its synergism with Xylanase SRXL1 was determined. Its activity was higher in a medium with corn hemicellulose and glucose as carbon sources. The purified protein was a monomer associated with the sr16700 gene, with a molecular weight of 117 kDa and optimal activity at 60 °C in a pH range of 4–7, which had the ability to hydrolyze the ρ-nitrophenyl β-D-xylanopyranoside and ρ-Nitrophenyl α-L-arabinofuranoside substrates. Its activity was strongly inhibited by silver ions and presented Km and Vmax values of 2.5 mM and 0.2 μmol/min/mg, respectively, using ρ-nitrophenyl β-D-xylanopyranoside as a substrate. The enzyme degrades corn hemicellulose and birch xylan in combination and in sequential synergism with the xylanase SRXL1.
Implementation of a Biometric-Based Blockchain System for Preserving Privacy, Security, and Access Control in Healthcare Records
Authors: Barka Ezedin , Al Baqari Mohammed , Kerrache Chaker Abdelaziz , Herrera-Tapia Jorge
Published: December 2022

The use of Electronic Health Record (EHR) systems has emerged with the continuous advancement of the Internet of Things (IoT) and smart devices. This is driven by the various advantages for both patients and healthcare providers, including timely and distant alerts, continuous control, and reduced cost, to name a few. However, while providing these advantages, various challenges involving heterogeneity, scalability, and network complexity are still open. Patient security, data privacy, and trust are also among the main challenges that need more research effort. To this end, this paper presents an implementation of a biometric-based blockchain EHR system (BBEHR), a prototype that uniquely identifies patients, enables them to control access to their EHRs, and ensures recoverable access to their EHRs. This approach overcomes the dependency on the private/public key approach used by most blockchain technologies to identify patients, which becomes more crucial in situations where a loss of the private key permanently hinders the ability to access patients’ EHRs. Our solution covers component selection, high-level implementation, and integration of subsystems, was well as the coding of a prototype to validate the mitigation of the risk of permanent loss of access to EHRs by using patients’ fingerprints. A performance analysis of BBEHR showed our system’s robustness and effectiveness in identifying patients and ensuring access control for their EHRs by using blockchain smart contracts with no additional overhead.
Behavioral Repertoire on a Vertical Rod—An Ethogram in Dermacentor reticulatus Ticks
Authors: Vargová Blažena , Pipová Natália , Baňas Miroslav , Majláth Igor , Tryjanowski Piotr , Jankowiak Łukasz , Majláthová Viktória
Published: December 2022

Ticks are important vectors of pathogens that endanger humans and animals. Study of their behavior under laboratory conditions is important for both predicting their behavior in natural conditions and understanding their involvement in transmission cycles of pathogens, which may lead to effective prevention of tick-borne disease transmission or establishment of effective preventive measures. The aim of our study was to describe the behavior of D. reticulatus ticks using laboratory assay. We focused on the description of individual behavioral units during their vertical movement. The assay consisted of glass beakers filled with sand and an embedded glass rod. We observed 10 different behavioral units, 4 of which have not yet been described: body posturing called “jogger”, leg grooming, and body or leg jerking. The most frequent tick behavior observed was an upwards positioning of the two front legs while the body remained motionless (88.9%). Other common observations were both horizontal (63%) and vertical (58.0%) body posturing with all legs lowered, followed by questing behavior (51.9%). Ticks spent the most time questing (75.2%), crawling (54.7%), and grooming legs on the right side (23%). We did not observe any differences between males and females.
SARS-CoV-2 Infection in Patients with Cystic Fibrosis: What We Know So Far
Authors: Biondo Carmelo , Midiri Angelina , Gerace Elisabetta , Zummo Sebastiana , Mancuso Giuseppe
Published: December 2022

Respiratory infections are the most common and most frequent diseases, especially in children and the elderly, characterized by a clear seasonality and with an incidence that usually tends to decrease with increasing age. These infections often resolve spontaneously, usually without the need for antibiotic treatment and/or with the possible use of symptomatic treatments aimed at reducing overproduction of mucus and decreasing coughing. However, when these infections occur in patients with weakened immune systems and/or underlying health conditions, their impact can become dramatic and in some cases life threatening. The rapid worldwide spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection has caused concern for everyone, becoming especially important for individuals with underlying lung diseases, such as CF patients, who have always paid close attention to implementing protective strategies to avoid infection. However, adult and pediatric CF patients contract coronavirus infection like everyone else. In addition, although numerous studies were published during the first wave of the pandemic on the risk for patients with cystic fibrosis (CF) to develop severe manifestations when infected with SARS-CoV-2, to date, a high risk has been found only for patients with poorer lung function and post-transplant status. In terms of preventive measures, vaccination remains key. The best protection for these patients is to strengthen preventive measures, such as social distancing and the use of masks. In this review, we aim to summarize and discuss recent advances in understanding the susceptibility of CF individuals to SARS-CoV-2 infection.
Long-Term Predictors of Hospitalized Reinfarction after an Incident Acute Myocardial Infarction
Authors: Schmitz Timo , Harmel Eva , Heier Margit , Peters Annette , Linseisen Jakob , Meisinger Christa
Published: December 2022

The aim of this study was to compare characteristics of incident acute myocardial infarction (AMI) and first and second time reinfarctions in terms of sociodemographic characteristics, comorbidities, symptoms, treatment, clinical characteristics, medication and outcome. A further aim was to identify predictors for an increased risk of hospitalized reinfarction. Between 2000 and 2017, a total of 13,276 AMI cases were recorded by a population-based registry in the area of Augsburg, Germany, and were included in this study (11,871 incident events, 1217 cases of first-time reinfarction and 202 cases of second-time reinfarction). Median follow-up time was 5.3 years. For differences in baseline characteristics, Chi-square tests and analysis of variance (ANOVA) were calculated. To determine factors that are associated with an increased risk of hospitalized reinfarction COX regression models were fitted. Myocardial reinfarctions differ from incident events in some major characteristics such as the frequency of comorbidities, laboratory values, ECG presentation and therapy, but not regarding 28-day mortality. Moreover, typical comorbidities and risk factors (diabetes, hypertension, hyperlipidemia, smoking, impaired renal function) are associated with an increased risk of hospitalized reinfarction. Conversely, STEMI ECG, being married, German nationality and bypass surgery are predictors for a lower risk of hospitalized reinfarction. Incident AMI and reinfarction are distinctly different in many characteristics, which physicians should have in mind when treating patients with prior AMI. Typical comorbidities are risk factors for hospitalized reinfarction. This underlines the importance of comprehensive treatment of these comorbidities including education of patients and encouragement towards lifestyle adjustments.
Mechanical Properties and Mechanism Analysis of Graphite Tailings Environment-Friendly Concrete
Authors: Duan Hourui , Liu Hongbo , Li Bochen , Wang Zhongrui , Gao Hongshuai
Published: December 2022

The development of tailings in concrete technology is not only conducive to the realization of the goal of reducing carbon emissions, but also conducive to the inhibition the occurrence of shortages of sand and gravel supplies. In this study, graphite tailings were used to replace sand in the range of 0~100%, and the mechanical mechanism of graphite tailings concrete was examined through compressive and flexural tests. The mechanical experimental results were evaluated and verified based on concrete macroscopic failure appearance, mesoscopic failure appearance, and physical characteristics of graphite tailings. The results revealed that the concrete strength increases first and then decreases with the increase of the graphite tailings content. Compared to GT00 (GT00 is a specimen with a graphite tailings content of 0%, and so on), GT10~GT60 exhibited better mechanical properties, of which 30% was recommended as the optimal replacement rate. The mechanical properties of GT10 and GT20 had an upward trend, and GT30 had low spalling, with aggregate fragmentation found on the fracture surface. GT30 showed the best resistance to bending and deformation. The mechanical properties of GT40~GT60 had a downward trend. When the graphite tailings content was higher than 70%, the interface defects of the aggregate matrix increased, thus making it easier for cracks to propagate along the interface. Furthermore, the mechanism of graphite tailings replacing sand verified the test results from different perspectives, which provides new analysis ideas for other tailings in environment-friendly concrete.
Polyaniline and Polyaniline-Based Materials as Sorbents in Solid-Phase Extraction Techniques
Authors: Sowa Ireneusz , Wójciak Magdalena , Tyszczuk-Rotko Katarzyna , Klepka Tomasz , Dresler Sławomir
Published: December 2022

Polyaniline (PANI) is one of the best known and widely studied conducting polymers with multiple applications and unique physicochemical properties. Due to its porous structure and relatively high surface area as well as the affinity toward many analytes related to the ability to establish different types of interactions, PANI has a great potential as a sorbent in sample pretreatment before instrumental analyses. This study provides an overview of the applications of polyaniline and polyaniline composites as sorbents in sample preparation techniques based on solid-phase extraction, including conventional solid-phase extraction (SPE) and its modifications, solid-phase microextraction (SPME), dispersive solid-phase extraction (dSPE), magnetic solid-phase extraction (MSPE) and stir-bar sorptive extraction (SBSE). The utility of PANI-based sorbents in chromatography was also summarized. It has been shown that polyaniline is willingly combined with other components and PANI-based materials may be formed in a variety of shapes. Polyaniline alone and PANI-based composites were successfully applied for sample preparation before determination of various analytes, both metal ions and organic compounds, in different matrices such as environmental samples, food, human plasma, urine, and blood.
Microstructure Evolution and Enhanced Hot Workability of TiC/Ti-6Al-4V Composites Fabricated by Melt Hydrogenation
Authors: Wang Xuan , Chen Siyu , Tan Yingmei , Yao Longhui , Wang Liang , Su Yanqing , Guo Jingjie
Published: December 2022

Improving the hot workability and reducing the processing cost are critical steps to expanding the application of TiC/Ti-6Al-4V composites. This study employed melt hydrogenation to fabricate TiC/Ti-6Al-4V composites under a mixed atmosphere of hydrogen and argon. Experimental results indicated that hydrogen had an obvious influence on the growth and morphology of eutectic TiC particles, and the size of eutectic TiC and primary β grains was significantly increased. As a result, large-sized eutectic TiC was distributed along the grain boundaries of primary β grains. Hot compression results showed that the peak flowing stress of composites was reduced by hydrogen, which resulted in an improvement of hot workability, especially in the (α + β) phase region, and the best results were obtained at 900 °C/0.01 s−1, at which the peak stress decreased from 241 ± 9 to 190 ± 8 MPa (a decrease of 21.2%). Inspection of the microstructure after hot compression showed that hydrogen improved the proportion of DRX grains from ~62.7% to ~83.2%, and hydrogen also decreased the density of dislocations, which were attributed to hydrogen accelerating atomic diffusion. Enhanced hot workability resulted from hydrogen atoms decreasing the atomic bonding force of the titanium matrix, hydrogen reducing the β/(α + β) transition temperature, the higher proportion of DRX, and the higher mobility of dislocations. It is expected that the findings of this study may support the development of a simple and efficient method to reduce the processing cost of TiC/Ti-6Al-4V composites.
Effect of Si and B on the Electrochemical Behavior of FeCoNiCr-Based High-Entropy Amorphous Alloys
Authors: Panahi S. Leila , Bruna Pere , Pineda Eloi
Published: December 2022

The ability to produce high-entropy alloys with an amorphous structure, so-called high-entropy metallic glasses (HEMGs), offers the possibility to produce new compositions with good mechanical properties and resistance to corrosion. In this study, corrosion behavior was studied in two HEMGs, FeCoNiCrB and FeCoNiCr(BSi). In both cases, the total amount of metalloid atoms was kept constant at 20 at.%. The electrochemical behavior of these alloys was studied by means of linear polarization resistance (LPR) measurements and electrochemical impedance spectroscopy in a 3 wt.% NaCl solution. The effect of corrosion was characterized by using X-ray photoelectron spectroscopy (XPS) and the surface morphology was checked using a scanning electron microscope (SEM). The results show that samples with B but without Si exhibit better corrosion resistance due to its chemical homogeneity and lack of structural heterogeneity.
Effect of Coolant Temperature on the Thermal Compensation of a Machine Tool
Authors: Maurya Swami Nath , Li Kun-Ying , Luo Win-Jet , Kao Shih-Ying
Published: December 2022

Machine tool (MT) accuracy is an important factor in the industry and is affected by heat generation through internal and external moving parts; the electrical components used; and variable environmental temperatures. Thermal errors lead to 40–60% of all MT errors. To improve MT accuracy, efficient techniques to minimize thermal errors must be identified. This study investigated the coolant temperature effects under different rotating speeds of a standalone built-in spindle system and computer numerical control (CNC) machine with a direct-drive spindle on the accuracy of thermal deformation prediction. The z-axis thermal deformation of the standalone built-in spindle system and CNC machine with a direct-drive spindle was conducted at different spindle rotating speeds and coolant temperatures at a constant coolant flow rate of 5 LPM. All experiments were conducted in a steady and dynamic operation according to ISO 230-3. For the standalone built-in spindle system, in comparison to the Mares model, the developed new model based on the coolant temperature effect on the Mares model (Mares CT model) can improve the thermal deformation prediction accuracy by 18.17% to 39.50% at different coolant temperatures of 12 ∘C to 26 ∘C and the accuracy can be controlled within the range of 0.03 μm to 5.24 μm, while the supply coolant temperature is above 16 ∘C. However, the thermal compensation analysis of the Mares CT model for a CNC machine with a direct-drive spindle shows a thermal deformation prediction accuracy improvement of 58.30% to 66.35% at different coolant temperatures of 22 ∘C to 28 ∘C and the accuracy can be controlled within the range of 0.14 μm to 4.05 μm. To validate the feasibility of the compensation model in real machining processes, dynamic operational analysis was performed for a standalone built-in spindle system and a CNC machine with a direct-drive spindle, and the thermal deformation prediction accuracy improved by 12.19% to 35.53% with the standalone built-in spindle system and 40.25% to 60.33% with the CNC machine with a direct-drive spindle. The compensation model analysis shows that the coolant temperature has a high impact on thermal deformation prediction and markedly affects system accuracy within certain limits.
Coherent Upper Conditional Previsions Defined through Conditional Aggregation Operators
Authors: Doria Serena
Published: December 2022

Conditional aggregation operators are introduced, and coherent upper conditional previsions are constructed by sub-additive, positively homogenous, and shift-invariant conditional aggregation operators. Composed operators defined by positively homogenous conditional aggregation operators are proven to be conditional aggregation operators and they are involved in the construction of coherent upper conditional previsions. The given results show that the composed conditional aggregation operator obtained as the supremum of the class of the Choquet integrals with respect to Hausdorff outer measures defined by bi-Lipschitz equivalent metrics is a coherent upper conditional prevision.
Perceptron: Learning, Generalization, Model Selection, Fault Tolerance, and Role in the Deep Learning Era
Authors: Du Ke-Lin , Leung Chi-Sing , Mow Wai Ho , Swamy M. N. S.
Published: December 2022

The single-layer perceptron, introduced by Rosenblatt in 1958, is one of the earliest and simplest neural network models. However, it is incapable of classifying linearly inseparable patterns. A new era of neural network research started in 1986, when the backpropagation (BP) algorithm was rediscovered for training the multilayer perceptron (MLP) model. An MLP with a large number of hidden nodes can function as a universal approximator. To date, the MLP model is the most fundamental and important neural network model. It is also the most investigated neural network model. Even in this AI or deep learning era, the MLP is still among the few most investigated and used neural network models. Numerous new results have been obtained in the past three decades. This survey paper gives a comprehensive and state-of-the-art introduction to the perceptron model, with emphasis on learning, generalization, model selection and fault tolerance. The role of the perceptron model in the deep learning era is also described. This paper provides a concluding survey of perceptron learning, and it covers all the major achievements in the past seven decades. It also serves a tutorial for perceptron learning.