bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Client-Centered Brain Injury Rehabilitation Through Research-Based Theater
Authors: Kontos Pia C. , Miller Karen-Lee , Gilbert Julie E. , Mitchell Gail J. , Colantonio Angela , Keightley Michelle L. , Cott Cheryl
Published: September 2012

Traumatic brain injury often results in physical, behavioral, and cognitive impairments perceived by health care practitioners to limit or exclude clients’ full participation in treatment decision making. We used qualitative methods to evaluate the short- and long-term impact of “After the Crash: A Play About Brain Injury,” a research-based drama designed to teach client-centered care principles to brain injury rehabilitation staff. We conducted interviews and observations with staff of two inpatient neurorehabilitation units in Ontario, Canada. Findings demonstrate the effectiveness of the play in influencing practice through the avoidance of medical jargon to improve clients’ understanding and participation in treatment; newfound appreciation for clients’ needs for emotional expression and sexual intimacy; increased involvement of family caregivers; and avoidance of staff discussions as if clients were unaware. These findings suggest that research-based drama can effect reflexivity, empathy, and practice change to facilitate a client-centered culture of practice in brain injury rehabilitation.
Deep-Level Team Composition and Innovation
Authors: Post Corinne
Published: September 2012

This study proposes and tests a process model linking deep-level team composition and innovation. Two cognitive styles, sequential thinking and connective thinking, are the deep-level team attributes hypothesized to affect team innovation. Psychological safety and cooperative learning are hypothesized to link cognitive styles and innovation at the team level. Findings from a sample of 83 natural innovation teams with dual-source data indicate that sequential thinking contributes to decreases in team innovation by inhibiting psychological safety, while connective thinking helps improve team innovation through increased cooperative learning. Implications for research and practice are discussed.
Parent–Child Interaction Therapy
Authors: Thomas Rae , Zimmer-Gembeck Melanie J.
Published: September 2012

It is common practice to augment efficacious treatment protocols for special populations (Durlak & DuPre, 2008), but this is often done before establishing that standard services are not appropriate. In this randomized controlled trial with families at risk or with a history of maltreatment ( N = 151), we investigated the effectiveness of standard 12-session Parent–Child Interaction Therapy (PCIT). This is in contrast to other PCIT studies with similar parents, which have allowed for longer and sometimes variable treatment length and with modifications to PCIT protocol. After treatment and compared to Waitlist, mothers reported fewer child externalizing and internalizing behaviors, decreased stress, and were observed to have more positive verbalizations and maternal sensitivity. These outcomes were equivalent or better than outcomes of our previous PCIT trial with high-risk families (Thomas & Zimmer-Gembeck, 2011) when treatment length was variable and often longer. These findings support standard protocol PCIT as an efficacious intervention for families in the child welfare system.
Attention Deficits in Children With Combined Autism and ADHD
Authors: Lundervold Astri J. , Stickert Marte , Hysing Mari , Sørensen Lin , Gillberg Christopher , Posserud Maj-Britt
Published: September 2012

Objective: To investigate characteristics of attention in children with the combination of autism spectrum disorder (ASD) and ADHD. Method: Four groups of 8- to 10-year-old children were compared on the Conners’ Continuous Performance Test–Second Edition (CCPT-II): (a) ASD + ADHD ( n = 11), (b) ASD only ( n = 9), (c) ADHD only ( n = 38), and (d) no diagnosis ( n = 134). Results: There was an overall effect of group on the Continuous Performance Test (CPT) index and measures of hit reaction time, accuracy, response style, variability, and consistency. The ASD + ADHD group, much like the ADHD only group, had a more risky response style, a higher variability, and a lower consistency than the ASD only group. The impact of intellectual function on CCPT-II performance was considerable in children within the ASD subgroups. Conclusion: The findings underscore the importance of including measures of attention and intellectual function when assessing children with the combination of ASD and ADHD.
Lubricity and corrosiveness of ionic liquids for steel-on-steel sliding contacts
Authors: Kondo Yuriko , Yagi Sotaro , Koyama Takahiro , Tsuboi Ryo , Sasaki Shinya
Published: September 2012

Ionic liquids are expected to be used as new high-performance lubricants because of their low volatility, high thermal stability, and high oxidation stability. It is well known that some ionic liquids exhibit excellent lubricity for metals. On the other hand, there is concern about the corrosiveness of ionic liquids caused by tribo-chemical reactions. In this study, the lubricating properties of seven kinds of commercially available ionic liquids were evaluated under steel-on-steel sliding contacts using an SRV oscillating sliding tester. The worn surfaces were analyzed by optical microscopy, confocal laser scanning microscopy, scanning electron microscopy with energy dispersive X-ray spectrometry, and X-ray photoelectron spectroscopy. The halogen-containing ionic liquids exhibited excellent lubricity for steel compared with the halogen-free ionic liquids. The X-ray photoelectron spectroscopy analytical results showed the formation of iron fluoride on sliding surfaces lubricated with the fluorine-containing ionic liquids. Formation of this iron-fluoride boundary layer reduced friction; however, corrosion occurred on the worn surfaces after the sliding. On the other hand, less corrosive damage was observed on the worn surfaces that slid under the dry-nitrogen atmospheric condition. These results suggested that the hydrofluoric acid, which was produced by the reaction between iron fluoride and contaminated water from the atmosphere, caused the corrosion on the worn surface. The halogen-free ionic liquids showed inferior lubricity compared with the halogen-containing ionic liquids, though they did not cause any remarkable corrosion. The corrosion-inhibition effect of the phosphorus element was shown in the halogen-containing ionic liquids composed of a phosphate anion or phosphonium cation. The friction-reduction effect was, however, inferior to that of the phosphorus-free ionic liquids. The tribo-chemical reaction of the phosphorus contained in the ionic liquids yielded a phosphate boundary layer that prevented the formation of iron halide on the sliding surface and enhanced the wear resistance.
A practical guide for using proper orthogonal decomposition in engine research
Authors: Chen Hao , Reuss David L , Hung David LS , Sick Volker
Published: September 2012

Proper orthogonal decomposition has been utilized for well over a decade to study turbulence and cyclic variation of flow and combustion properties in internal combustion engines. In addition, proper orthogonal decomposition is useful to quantitatively compare multi-cycle in-cylinder measurements with numerical simulations (large-eddy simulations). However, the application can be daunting, and physical interpretation of proper orthogonal decomposition can be ambiguous. In this paper, the mathematical procedure of proper orthogonal decomposition is described conceptually, and a compact MATLAB® code is provided. However, the major purpose is to empirically illustrate the properties of the proper orthogonal decomposition analysis and to propose practical procedures for application to internal combustion engine flows. Two measured velocity data sets from a motored internal combustion engine are employed, one a highly directed flow (each cycle resembles the ensemble average), and the other an undirected flow (no cycle resembles the average). These data are used to illustrate the degree to which proper orthogonal decomposition can quantitatively distinguish between internal combustion engine flows with these two extreme flow properties. In each flow, proper orthogonal decomposition mode 1 is an excellent estimate of ensemble average, and this study illustrates how it is thus possible to unambiguously quantify the cyclic variability of Reynolds-averaged Navier–Stokes ensemble average and turbulence. In addition, this study demonstrates the benefits of comparing two different samples of cycles using a common proper orthogonal decomposition mode set derived by combining the two samples, the effect of spatial resolution, and a method to evaluate the number of snapshots required to achieve convergence.
Doing participatory qualitative research: development of a shared critical consciousness with racial minority research advisory group members
Authors: Maiter Sarah , Joseph Ameil J , Shan Neethan , Saeid Aqeel
Published: September 2012

Partnership and principles of self-determination, equity, and social justice are keys to community-based participatory research that aims to break down barriers between the researchers and researched. Because community partners are valued as equal contributors to the research project, research advisory groups consisted of members of the community researched are essential to this approach. We discuss the challenges that arose in our first meeting with research advisory group members, where discussions of experiences of racism, in sharing the topic of the research, were initiated with youth from a diverse racialized community. We discuss the ‘development of a shared critical consciousness’, one that incorporated both the researcher’s reaction and the research advisory group members’ development (from initial resistance to critical consciousness). This approach respects the richness of the growth process during research, values the contributions of the researchers and the researched, and via the dialogical process captures contribution in a way that does not finalize or determine people.
Rasch Validation of a Combined Measure of Basic and Extended Daily Life Functioning After Stroke
Authors: Chen Hui-fang , Wu Ching-yi , Lin Keh-chung , Chen Chia-ling , Huang Pai-chuan , Hsieh Ching-ju , Liu Jung-sen
Published: September 2012

Background. Tools used to measure poststroke functional status must include basic and instrumental activities of daily living and reflect the patient’s and the clinician’s perspective of the disease and its effect on daily living performance. Objective. The authors combined the Functional Independence Measure (FIM) and the Nottingham Extended Activities of Daily Living (NEADL) to create a scale providing a comprehensive evaluation of ADLs functional status in patients with stroke. Methods. The study participants were 188 patients completing the FIM and the NEADL. The psychometric properties of the combined measure were examined with Rasch analysis. Results. A 3-point scale and a dichotomous scale were suggested for use in the FIM and the NEADL, respectively. The combined 40 items worked consistently to reflect a single construct, and “bladder management” and “bowel management” were highly related. After “bowel management” was removed from the combined scale, all but 3 items fit the model’s expectations, and the 39-item scale showed reasonable item difficulty hierarchy, with high reliability. The 3 misfit items were removed, and no differences in unidimensionality, differential item functioning, and reliability were found between the 36-item and 39-item scales. Conclusions. The combined measure of the FIM and the NEADL provides a comprehensive picture of ADLs. It extends the utility of the FIM and the NEADL and is recommended for use to measure the independence of patients after discharge home.
Balancing risk and professional identity, secondary school teachers’ narratives of violence
Authors: Martin Denise , Mackenzie Nicola , Healy Jane
Published: September 2012

Violence against teachers is seen as a growing problem by both professional bodies and the media. However, this account fails to acknowledge differing views about what actually constitutes violence and how those that experience violence comprehend it. Drawing on literature on workplace violence and fear of crime, this article seeks to identify how we can begin to understand better violence against teachers. Furthermore, by examining secondary school teachers’ own narratives in depth, it is identified that a number of factors influence the meanings that they attach to their own experiences of workplace violence. This includes their professional identity, feelings about their pupils and their role as a teacher, their own sense of vulnerability, levels of experience and general feelings about schools and young people today.
Irreducible Dislocation of All the Lesser Metatarsophalangeal Joints of the Foot
Authors: Neogi Devdatta Suhas , Bandekar Shivanand M. , Sadekar Vilas , Patnaik Sarthak , Bhat Trivikram , D’Mello Zelio
Published: September 2012

We report a case of irreducible dorsal dislocation of all the lesser metatarsophalangeal joints of the foot following injury at sport. Plantar plate prevented reduction at all the joints, which necessitated an open reduction and stabilization with K-wire. This report highlights the necessity of prompt open reduction and reviews the mechanism of injury and complex anatomy at metatarsophalangeal joint.

Levels of Evidence: Therapeutic, Level IV, Case study
An integrated approach in the diagnosis of smoking-related interstitial lung diseases
Authors: Caminati Antonella , Cavazza Alberto , Sverzellati Nicola , Harari Sergio
Published: September 2012

Cigarette smoke consists of several chemical compounds with a variety of effects in many organs. In the lung, apart being the main cause of chronic obstructive pulmonary disease, carcinoma and idiopathic spontaneous pneumothorax, tobacco smoke is associated with interstitial lung diseases (ILDs), including respiratory bronchiolitis-associated ILD (RB-ILD), desquamative interstitial pneumonia (DIP), pulmonary Langerhans’ cell histiocytosis (PLCH), idiopathic pulmonary fibrosis, acute eosinophilic pneumonia, ILD in rheumatoid arthritis and pulmonary haemorrhage in Goodpasture syndrome.

This review will focus on the diseases with a stronger epidemiological association with tobacco smoke, namely RB-ILD, DIP and PLCH. Although the exact pathogenetic evidence linking smoking with these disorders is still not completely understood, there is growing evidence that tobacco smoke targets the terminal or respiratory bronchioles in these diseases, and the differences are reflective of the degree of severity of small airway and parenchymal reaction to the smoke exposure.

Despite considerable clinical, radiological and histological overlap between RB-ILD, DIP and PLCH, it is useful to retain the separate classifications for prognostic and therapeutic implications.
A critical analysis of survival in pulmonary arterial hypertension
Authors: O'Callaghan Dermot S. , Humbert Marc
Published: September 2012

Pulmonary arterial hypertension (PAH) is a rare disease characterised by a progressive increase in pulmonary vascular resistance, leading to right heart failure and premature death. Over the last two decades, a better understanding of the pathogenesis of PAH has led to the approval of several targeted therapies that confer improvements in patients’ clinical and haemodynamic status, quality of life and survival. Analysis of recent survival data in PAH cohorts have been biased by the inclusion of prevalent patients included months or years after PAH diagnosis (“survivors”) who have a better prognosis than corresponding incident patients diagnosed at the time of recruitment. A critical analysis of recent multicentre incident cohorts has shown survival improvements in the current management era. However, idiopathic PAH remains a severe disease and still carries a poor prognosis. Modern survival figures also highlight the relevance of lung transplantation in eligible PAH patients who are refractory to current best standard of medical care.
Echocardiographic assessment of pulmonary hypertension: standard operating procedure
Authors: Howard Luke S. , Grapsa Julia , Dawson David , Bellamy Michael , Chambers John B. , Masani Navroz D. , Nihoyannopoulos Petros , Simon R. Gibbs J.
Published: September 2012

Patients with suspected pulmonary hypertension (PH) should be evaluated using a multimodality approach to ensure that they receive a correct diagnosis. The series of investigations required includes clinical evaluation, noninvasive imaging techniques and right heart catheterisation (considered to be the “gold standard” for the diagnosis of PH). Current guidelines recommend that a detailed echocardiographic assessment is performed in all patients with suspected PH.

In this review we summarise a protocol adopted by the National Pulmonary Hypertension Centres of UK and Ireland and approved by the British Society of Echocardiography for the evaluation of these patients. The views and measurements described are recommended for diagnosis, assisting in prognosis and providing a noninvasive means of following disease progression or response to therapy.
Portopulmonary hypertension and hepatopulmonary syndrome: a clinician-oriented overview
Authors: Porres-Aguilar Mateo , Altamirano Jose T. , Torre-Delgadillo Aldo , Charlton Michael R. , Duarte-Rojo Andres
Published: September 2012

Liver disease and portal hypertension can be associated with pulmonary vascular complications, including portopulmonary hypertension (POPH), characterised by an elevated mean pulmonary artery pressure secondary to an increased pulmonary vascular resistance, and hepatopulmonary syndrome (HPS), characterised by hypoxaemia due to pulmonary vasodilatation and shunting.

Although clear diagnostic guidelines exist for both conditions on the basis of echocardiography, right heart catheterisation and arterial blood gases, there is considerable variation between centres regarding diagnosis and management of these conditions. Awareness of evaluation and management algorithms for POPH and HPS are critical for optimisation of outcomes in patients with these conditions.

Key aspects of management of POPH and HPS include identification of patients likely to benefit from liver transplantation (LTx) and management before and after LTx. Although both disorders may improve after LTx, severe forms of POPH represent a contraindication to LTx.

Novel approaches to the treatment of POPH and HPS offer new management options that may expand the pool of transplantable patients and improve overall outcomes.
Lymphatics in lymphangioleiomyomatosis and idiopathic pulmonary fibrosis
Authors: Glasgow Connie G. , El-Chemaly Souheil , Moss Joel
Published: September 2012

The primary function of the lymphatic system is absorbing and transporting macromolecules and immune cells to the general circulation, thereby regulating fluid, nutrient absorption and immune cell trafficking. Lymphangiogenesis plays an important role in tissue inflammation and tumour cell dissemination. Lymphatic involvement is seen in lymphangioleiomyomatosis (LAM) and idiopathic pulmonary fibrosis (IPF).

LAM, a disease primarily affecting females, involves the lung (cystic destruction), kidney (angiomyolipoma) and axial lymphatics (adenopathy and lymphangioleiomyoma). LAM occurs sporadically or in association with tuberous sclerosis complex (TSC). Cystic lung destruction results from proliferation of LAM cells, which are abnormal smooth muscle-like cells with mutations in theTSC1orTSC2gene. Lymphatic abnormalities arise from infiltration of LAM cells into the lymphatic wall, leading to damage or obstruction of lymphatic vessels. Benign appearing LAM cells possess metastatic properties and are found in the blood and other body fluids.

IPF is a progressive lung disease resulting from fibroblast proliferation and collagen deposition. Lymphangiogenesis is associated with pulmonary destruction and disease severity. A macrophage subset isolated from IPF bronchoalveolar lavage fluid (BALF) express lymphatic endothelial cell markersin vitro, in contrast to the same macrophage subset from normal BALF.

Herein, we review lymphatic involvement in LAM and IPF.
A genome-wide identification and analysis of the basic helix-loop-helix transcription factors in the ponerine ant, Harpegnathos saltator
Authors: Liu Ake , Wang Yong , Dang Chunwang , Zhang Debao , Song Huifang , Yao Qin , Chen Keping
Published: September 2012

Background

The basic helix-loop-helix (bHLH) transcription factors and their homologs form a superfamily that plays essential roles in transcriptional networks of multiple developmental processes. bHLH family members have been identified in over 20 organisms, including fruit fly, zebrafish, human and mouse.

Result

In this study, we conducted a genome-wide survey for bHLH sequences, and identified 57 bHLH sequences encoded in complete genome sequence of the ponerine ant, Harpegnathos saltator. Phylogenetic analysis of the bHLH domain sequences classified these genes into 38 bHLH families with 23, 14, 10, 1, 8 and 1 members in group A, B, C, D, E and F, respectively. The number of PabHLHs (ponerine ant bHLHs) with introns is higher than many other insect species, and they are found to have introns with average lengths only inferior to those of pea aphid. In addition, two H. saltator bHLHs named PaCrp1 and PaSide locate on two separate contigs in the genome.

Conclusions

A putative full set of PabHLH genes is comparable with other insect species and genes encoding Oligo, MyoRb and Figα were not found in genomes of all insect species of which bHLH family members have been identified. Moreover, in-family phylogenetic analyses indicate that the PabHLH genes are more closely related with Apis mellifera than others. The present study will serve as a solid foundation for further investigations into the structure and function of bHLH proteins in the regulation of H. saltator development.
Polyphyletic origin of the genus Physarum(Physarales, Myxomycetes) revealed by nuclear rDNA mini-chromosome analysis and group I intron synapomorphy
Authors: Nandipati Satish CR , Haugli Kari , Coucheron Dag H , Haskins Edward F , Johansen Steinar D
Published: September 2012

Background

Physarales represents the largest taxonomic order among the plasmodial slime molds (myxomycetes). Physarales is of particular interest since the two best-studied myxomycete species,Physarum polycephalumandDidymium iridis, belong to this order and are currently subjected to whole genome and transcriptome analyses. Here we report molecular phylogeny based on ribosomal DNA (rDNA) sequences that includes 57 Physarales isolates.

Results

The Physarales nuclear rDNA sequences were found to be loaded with 222 autocatalytic group I introns, which may complicate correct alignments and subsequent phylogenetic tree constructions. Phylogenetic analysis of rDNA sequences depleted of introns confirmed monophyly of the Physarales families Didymiaceae and Physaraceae. Whereas good correlation was noted between phylogeny and taxonomy among the Didymiaceae isolates, significant deviations were seen in Physaraceae. The largest genus,Physarum, was found to be polyphyletic consisting of at least three well supported clades. A synapomorphy, located at the highly conserved G-binding site of L2449 group I intron ribozymes further supported thePhysarumclades.

Conclusions

Our results provide molecular relationship of Physarales genera, species, and isolates. This information is important in further interpretations of comparative genomics nd transcriptomics. In addition, the result supports a polyphyletic origin of the genusPhysarumand calls for a reevaluation of current taxonomy.
Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting
Authors: Ji Bo , Ernest Ben , Gooding Jessica R , Das Suchita , Saxton Arnold M , Simon Jean , Dupont Joelle , Métayer-Coustard Sonia , Campagna Shawn R , Voy Brynn H
Published: September 2012

Background

Domestic broiler chickens rapidly accumulate adipose tissue due to intensive genetic selection for rapid growth and are naturally hyperglycemic and insulin resistant, making them an attractive addition to the suite of rodent models used for studies of obesity and type 2 diabetes in humans. Furthermore, chicken adipose tissue is considered as poorly sensitive to insulin and lipolysis is under glucagon control. Excessive fat accumulation is also an economic and environmental concern for the broiler industry due to the loss of feed efficiency and excessive nitrogen wasting, as well as a negative trait for consumers who are increasingly conscious of dietary fat intake. Understanding the control of avian adipose tissue metabolism would both enhance the utility of chicken as a model organism for human obesity and insulin resistance and highlight new approaches to reduce fat deposition in commercial chickens.

Results

We combined transcriptomics and metabolomics to characterize the response of chicken adipose tissue to two energy manipulations, fasting and insulin deprivation in the fed state. Sixteen to 17 day-old commercial broiler chickens (ISA915) were fedad libitum, fasted for five hours, or fed but deprived of insulin by injections of anti-insulin serum. Pair-wise contrasts of expression data identified a total of 2016 genes that were differentially expressed after correction for multiple testing, with the vast majority of differences due to fasting (1780 genes). Gene Ontology and KEGG pathway analyses indicated that a short term fast impacted expression of genes in a broad selection of pathways related to metabolism, signaling and adipogenesis. The effects of insulin neutralization largely overlapped with the response to fasting, but with more modest effects on adipose tissue metabolism. Tissue metabolomics indicated unique effects of insulin on amino acid metabolism.

Conclusions

Collectively, these data provide a foundation for further study into the molecular basis for adipose expansion in commercial poultry and identify potential pathways through which fat accretion may be attenuated in the future through genetic selection or management practices. They also highlight chicken as a useful model organism in which to study the dynamic relationship between food intake, metabolism, and adipose tissue biology.
Effect of statin therapy on the progression of coronary atherosclerosis
Authors: Tian Jinwei , Gu Xia , Sun Yanli , Ban Xiang , Xiao Yun , Hu Sining , Yu Bo
Published: September 2012

Background

An increasing number of authors employing intravascular ultrasound (IVUS) and virtual histology (VH-IVUS) have investigated the effect of statin use on plaque volume (PV) and plaque composition. However, inconsistent results have been reported. Therefore, we conducted a meta-analysis to determine the appropriate regimen of statins to effectively stabilize vulnerable coronary plaques.

Methods

Online electronic databases were carefully searched for all relevant studies. We compared mean values of PV and plaque composition between baseline and follow-up in patients receiving statin therapy. We pooled treatment effects and calculated mean differences (MD) with the 95% confidence interval (CI) using a random-effects model. By stratified analyses, we explored the influence of clinical presentation, dose and duration of statin treatment, and low-density lipoprotein-cholesterol (LDL-C) levels on the effects of statins.

Results

Seventeen studies involving 2,171 patients were analyzed. Statin therapy significantly decreased PV (−5.3 mm3; 95% CI: –3.3 mm3 to −7.2 mm3; P < 0.001), without heterogeneity. When considering the dose and duration of statins used, only subgroups employing a high dose and long duration demonstrated a significant reduction in PV (p < 0.001). A significant decrease in PV was noted if achieved LDL-C levels were <100 mg/dL (p < 0.001). Statin treatment could induce a twofold decrease in PV in patients with acute coronary syndrome (ACS) compared with that observed in patients with stable angina pectoris (SAP). A regressive trend was seen for necrotic core volume (MD: –2.1 mm3; 95% CI: –4.7 mm3 to 0.5 mm3, P = 0.11). However, statin use did not induce a significant change for fibrotic, fibro-fatty, or dense calcium compositions.

Conclusions

Our meta-analysis demonstrated that statin therapy (especially that involving a high dose and long duration and achieving <100 mg/dL LDL-C levels) can significantly decrease PV in patients with SAP or ACS. These data suggested that statins can be used to reduce the atheroma burden for secondary prevention by appropriately selecting the statin regimen. No significant change in plaque composition was seen after statin therapy.
The contribution of the ABCG2 C421A polymorphism to cancer susceptibility: a meta-analysis of the current literature
Authors: Chen Pin , Zhao Lin , Zou Peng , Xu Haitao , Lu Ailin , Zhao Peng
Published: September 2012

Background

ABCG2, also known as BCRP, is a half ATP-binding cassette (ABC) transporter that localizes to plasma membranes. Recently, a number of studies have investigated the relationship between the C421A polymorphism in ABCG2 and cancer risk in multiple populations and various types of cancers; however, this relationship remains unclear. Therefore, we performed a meta-analysis to further explore this association.

Methods

The meta-analysis incorporated 10 studies involving a total of 3593 cases and 5875 controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated based on the date extracted from the studies to evaluate the strength of association. We also analyzed the heterogeneity and sensitivity of each report and the publication bias of the studies.

Results

Overall, our results showed that there appeared to be a significant association between the ABCG2 C421A polymorphism and decreased cancer susceptibility (heterozygote-AC versus CC: OR = 0.759, 95%CI = 0.620-0.930; dominant effects model-AA/AC versus CC: OR = 0.771, 95%CI = 0.634-0.938; additive effects model-A allele versus C allele: OR = 0.809, 95%CI = 0.687-0.952). Similarly, decreased cancer risk was also found after stratification of the SNP data by cancer type, ethnicity and source of controls in heterozygote model, dominant effects model and additive effects model.

Conclusions

We found that the ABCG2 C421A polymorphism is a protective factor for developing cancer. The same relationship was found when the studies were stratified by cancer type, ethnicity and source of controls.
Recurrent orbital schwannomas: clinical course and histopathologic correlation
Authors: Kron Michelle , Bohnsack Brenda L , Archer Steven M , McHugh Jonathan B , Kahana Alon
Published: September 2012

Background

Schwannomas are slow-growing typically encapsulated tumors composed of differentiated Schwann cells, the primary class of peripheral glial cells. Complete excision is the treatment of choice for orbital schwannomas that cause pain, disfigurement, diplopia, or optic neuropathy. The presence of multiple schwannomas in a single patient suggests possible association with neurofibromatosis type 2 (NF2) or schwannomatosis.

Case presentation

We present 2 patients who experienced recurrent orbital schwannoma without evidence for neurofibromatosis. The recurrence in one patient, a 59-year old man, occurred 6 years after complete excision of the initial tumor. This recurrence consisted of 2 independent tumors in the same orbit. The recurrence in the second patient, a 5 year-old girl, occurred multiple times within days to weeks of partial excisions until eventually a complete excision was performed.

Conclusion

The clinical history, histopathologic features and particularly the intraoperative findings suggest that the 59 year old man suffers from orbital schwannomatosis, while the rapid recurrence in the second patient correlated with the cellular features of her plexiform schwannoma. Hence, the recurrence in each patient is linked to a different etiology, with implications for treatment and patient counseling given the difficulty in treating orbital schwannomatosis. To our knowledge, this is the first description of isolated orbital schwannomatosis.
Determining the use of prophylactic antibiotics in breast cancer surgeries: a survey of practice
Authors: Acuna Sergio A , Angarita Fernando A , Escallon Jaime , Tawil Mauricio , Torregrosa Lilian
Published: September 2012

Background

Prophylactic antibiotics (PAs) are beneficial to breast cancer patients undergoing surgery because they prevent surgical site infection (SSI), but limited information regarding their use has been published. This study aims to determine the use of PAs prior to breast cancer surgery amongst breast surgeons in Colombia.

Methods

An online survey was distributed amongst the breast surgeon members of the Colombian Association of Mastology, the only breast surgery society of Colombia. The scope of the questions included demographics, clinical practice characteristics, PA prescription characteristics, and the use of PAs in common breast surgical procedures.

Results

The survey was distributed amongst eighty-eight breast surgeons of whom forty-seven responded (response rate: 53.4%). Forty surgeons (85.1%) reported using PAs prior to surgery of which >60% used PAs during mastectomy, axillary lymph node dissection, and/or breast reconstruction. Surgeons reported they targeted the use of PAs in cases in which patients had any of the following SSI risk factors: diabetes mellitus, drainsin situ, obesity, and neoadjuvant therapy. The distribution of the self-reported PA dosing regimens was as follows: single pre-operative fixed-dose (27.7%), single preoperative dose followed by a second dose if the surgery was prolonged (44.7%), single preoperative dose followed by one or more postoperative doses for >24 hours (10.6%), and single preoperative weight-adjusted dose (2.1%).

Conclusion

Although this group of breast surgeons is aware of the importance of PAs in breast cancer surgery there is a discrepancy in how they use it, specifically with regards to prescription and timeliness of drug administration. Our findings call for targeted quality-improvement initiatives, such as standardized national guidelines, which can provide sufficient evidence for all stakeholders and therefore facilitate best practice medicine for breast cancer surgery.
Psychometric properties of the Confidence and Trust in Delivery Questionnaire (CTDQ): a pilot study
Authors: Jeschke Elke , Ostermann Thomas , Dippong Natalie , Brauer Dagmar , Matthes Harald
Published: September 2012

Background

Assessing expecting mother’s opinions prior to birth draws a comprehensive picture for the caregivers about their emotional state and their expectations. Some questionnaires to cover these aspects do exist. This study aims to present the psychometric properties of a new instrument, the Confidence and Trust in Delivery Questionnaire (CDTQ) a short but reliable a self-report instrument that focuses on confidence and trust as meaningful dimensions for expectant mothers.

Methods

A pilot validation study of 221 women 6 weeks before childbirth was conducted in Germany between October 2007 and June 2008. To detect structural relations between the items, factor and reliability analyses were applied to the CTDQ items. Factor analysis was performed by means of principal components analysis and varimax rotation. Internal reliability was assessed by Cronbach’s alpha. External validation was performed using the sense of coherence (SOC) scale.

Results

The CTDQ comprises of 11 items. We found a 4-factor structure. The internal consistency of the whole item pool (Cronbach’s α = 0.79) and the 4 subscales [confidence in labor (α = 0.82); partner’s support (α = 0.62); trust in medical competency (α = 0.68); being informed (α = 0.60)] can be regarded as sufficient or even excellent. The 4 factors explained 69.6% of total variance. Except for a high intercorrelation (0.70) between “partner’s support” and “trust in medical competence”, the subscales show low intercorrelations, indicating an adequate independence of the respective subscales. Regarding the external validity we found minor respective moderate correlations with the SOC scale.

Conclusions

Our data suggest that the CTDQ is a useful instrument to assess confidence and trust in delivery. With 4 clinically relevant dimensions, the CTDQ is now open for further studies in the field of labor.
Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidative stress
Authors: Yeh Chi-Chen , Yang Jing-Iong , Lee Jin-Ching , Tseng Chao-Neng , Chan Ya-Ching , Hseu You-Cheng , Tang Jen-Yang , Chuang Li-Yeh , Huang Hurng-Wern , Chang Fang-Rong , Chang Hsueh-Wei
Published: September 2012

Background

Methanolic extracts of Gracilaria tenuistipitata (MEGT) were obtained from the edible red algae. Previously, we found that water extract of G. tenuistipitata was able to modulate oxidative stress-induced DNA damage and its related cellular responses.

Methods

In this study, the methanol extraction product MEGT was used to evaluate the cell growth inhibition in oral cancer cells and its possible mechanism was investigated.

Results

The cell viability of MEGT treated Ca9-22 oral cancer cell line was significantly decreased in a dose–response manner (p < 0.05). The sub-G1 population and annexin V intensity of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.0005 and p < 0.001, respectively). The γH2AX intensities of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.05). The reactive oxygen species (ROS) and glutathione (GSH)-positive intensities of MEGT-treated Ca9-22 oral cancer cells were significantly increased and decreased, respectively, in a dose–response manner (p < 0.05). The DiOC2(3) intensity for mitochondrial membrane potential (MMP) of MEGT-treated Ca9-22 cancer cells was significantly decreased in a dose–response manner (p < 0.05).

Conclusions

These results indicated that MEGT had apoptosis-based cytotoxicity against oral cancer cells through the DNA damage, ROS induction, and mitochondrial depolarization. Therefore, MEGT derived from the edible algae may have potential therapeutic effects against oral squamous cell carcinoma (OSCC).
Scale up, optimization and stability analysis of Curcumin C3 complex-loaded nanoparticles for cancer therapy
Authors: Ranjan Amalendu P , Mukerjee Anindita , Helson Lawrence , Vishwanatha Jamboor K
Published: September 2012

Background

Nanoparticle based delivery of anticancer drugs have been widely investigated. However, a very important process for Research & Development in any pharmaceutical industry is scaling nanoparticle formulation techniques so as to produce large batches for preclinical and clinical trials. This process is not only critical but also difficult as it involves various formulation parameters to be modulated all in the same process.

Methods

In our present study, we formulated curcumin loaded poly (lactic acid-co-glycolic acid) nanoparticles (PLGA-CURC). This improved the bioavailability of curcumin, a potent natural anticancer drug, making it suitable for cancer therapy. Post formulation, we optimized our process by Reponse Surface Methodology (RSM) using Central Composite Design (CCD) and scaled up the formulation process in four stages with final scale-up process yielding 5 g of curcumin loaded nanoparticles within the laboratory setup. The nanoparticles formed after scale-up process were characterized for particle size, drug loading and encapsulation efficiency, surface morphology, in vitro release kinetics and pharmacokinetics. Stability analysis and gamma sterilization were also carried out.

Results

Results revealed that that process scale-up is being mastered for elaboration to 5 g level. The mean nanoparticle size of the scaled up batch was found to be 158.5 ± 9.8 nm and the drug loading was determined to be 10.32 ± 1.4%. The in vitro release study illustrated a slow sustained release corresponding to 75% drug over a period of 10 days. The pharmacokinetic profile of PLGA-CURC in rats following i.v. administration showed two compartmental model with the area under the curve (AUC0-∞) being 6.139 mg/L h. Gamma sterilization showed no significant change in the particle size or drug loading of the nanoparticles. Stability analysis revealed long term physiochemical stability of the PLGA-CURC formulation.

Conclusions

A successful effort towards formulating, optimizing and scaling up PLGA-CURC by using Solid-Oil/Water emulsion technique was demonstrated. The process used CCD-RSM for optimization and further scaled up to produce 5 g of PLGA-CURC with almost similar physicochemical characteristics as that of the primary formulated batch.
Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS
Authors: Pham Kimberly , Langlais Paul , Zhang Xiangmin , Chao Alex , Zingsheim Morgan , Yi Zhengping
Published: September 2012

Background

Protein phosphatase 1 (PP1) is one of the major phosphatases responsible for protein dephosphorylation in eukaryotes. Protein phosphatase 1 regulatory subunit 12B (PPP1R12B), one of the regulatory subunits of PP1, can bind to PP1cδ, one of the catalytic subunits of PP1, and modulate the specificity and activity of PP1cδ against its substrates. Phosphorylation of PPP1R12B on threonine 646 by Rho kinase inhibits the activity of the PP1c-PPP1R12B complex. However, it is not currently known whether PPP1R12B phosphorylation at threonine 646 and other sites is regulated by insulin. We set out to identify phosphorylation sites in PPP1R12B and to quantify the effect of insulin on PPP1R12B phosphorylation by using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.

Results

14 PPP1R12B phosphorylation sites were identified, 7 of which were previously unreported. Potential kinases were predicted for these sites. Furthermore, relative quantification of PPP1R12B phosphorylation sites for basal and insulin-treated samples was obtained by using peak area-based label-free mass spectrometry of fragment ions. The results indicate that insulin stimulates the phosphorylation of PPP1R12B significantly at serine 29 (3.02 ± 0.94 fold), serine 504 (11.67 ± 3.33 fold), and serine 645/threonine 646 (2.34 ± 0.58 fold).

Conclusion

PPP1R12B was identified as a phosphatase subunit that undergoes insulin-stimulated phosphorylation, suggesting that PPP1R12B might play a role in insulin signaling. This study also identified novel targets for future investigation of the regulation of PPP1R12B not only in insulin signaling in cell models, animal models, and in humans, but also in other signaling pathways.
Neuronal c-Abl activation leads to induction of cell cycle and interferon signaling pathways
Authors: Schlatterer Sarah D , Suh Hyeon-sook , Conejero-Goldberg Concepcion , Chen Shufen , Acker Christopher M , Lee Sunhee C , Davies Peter
Published: September 2012

Background

Expression of active c-Abl in adult mouse forebrain neurons in the AblPP/tTA mice resulted in severe neurodegeneration, particularly in the CA1 region of the hippocampus. Neuronal loss was preceded and accompanied by substantial microgliosis and astrocytosis. In contrast, expression of constitutively active Arg (Abl-related gene) in mouse forebrain neurons (ArgPP/tTA mice) caused no detectable neuronal loss or gliosis, although protein expression and kinase activity were at similar levels to those in the AblPP/tTA mice.

Methods

To begin to elucidate the mechanism of c-Abl-induced neuronal loss and gliosis, gene expression analysis of AblPP/tTA mouse forebrain prior to development of overt pathology was performed. Selected results from gene expression studies were validated with quantitative reverse transcription PCR , immunoblotting and bromodeoxyuridine (BrdU) labeling, and by immunocytochemistry.

Results

Two of the top pathways upregulated in AblPP/tTA mice with c-Abl expression for 2 weeks were cell cycle and interferon signaling. However, only the expression of interferon signaling pathway genes remained elevated at 4 weeks of c-Abl induction. BrdU incorporation studies confirm that, while the cell cycle pathway is upregulated in AblPP/tTA mice at 2 weeks of c-Abl induction, the anatomical localization of the pathway is not consistent with previous pathology seen in the AblPP/tTA mice. Increased expression and activation of STAT1, a known component of interferon signaling and interferon-induced neuronal excitotoxicity, is an early consequence of c-Abl activation in AblPP/tTA mice and occurs in the CA1 region of the hippocampus, the same region that goes on to develop severe neurodegenerative pathology and neuroinflammation. Interestingly, no upregulation of gene expression of interferons themselves was detected.

Conclusions

Our data suggest that the interferon signaling pathway may play a role in the pathologic processes caused by c-Abl expression in neurons, and that the AblPP/tTA mouse may be an excellent model for studying sterile inflammation and the effects of interferon signaling in the brain.
Individualized, discrete event, simulations provide insight into inter- and intra-subject variability of extended-release, drug products
Authors: Kim Sean HJ , Jackson Andre J , Hur Rim , Hunt C Anthony
Published: September 2012

Objective

Develop and validate particular, concrete, and abstract yet plausible in silico mechanistic explanations for large intra- and interindividual variability observed for eleven bioequivalence study participants. Do so in the face of considerable uncertainty about mechanisms.

Methods

We constructed an object-oriented, discrete event model called subject (we use small caps to distinguish computational objects from their biological counterparts). It maps abstractly to a dissolution test system and study subject to whom product was administered orally. A subject comprises four interconnected grid spaces and event mechanisms that map to different physiological features and processes. Drugs move within and between spaces. We followed an established, Iterative Refinement Protocol. Individualized mechanisms were made sufficiently complicated to achieve prespecified Similarity Criteria, but no more so. Within subjects, the dissolution space is linked to both a product-subject Interaction Space and the GI tract. The GI tract and Interaction Space connect to plasma, from which drug is eliminated.

Results

We discovered parameterizations that enabled the eleven subject simulation results to achieve the most stringent Similarity Criteria. Simulated profiles closely resembled those with normal, odd, and double peaks. We observed important subject-by-formulation interactions within subjects.

Conclusion

We hypothesize that there were interactions within bioequivalence study participants corresponding to the subject-by-formulation interactions within subjects. Further progress requires methods to transition currently abstract subject mechanisms iteratively and parsimoniously to be more physiologically realistic. As that objective is achieved, the approach presented is expected to become beneficial to drug development (e.g., controlled release) and to a reduction in the number of subjects needed per study plus faster regulatory review.