bluuebunny/crossref_metadata_2025 huggingface dataset:
Intravenous Ascorbic Acid and Lung Function in Severely Ill COVID-19 Patients
Authors: Sokary Sara , Ouagueni Asma , Ganji Vijay
Published: September 2022

Current evidence suggests that ascorbic acid improves the host’s immune system and, therefore, may play a role in reducing the severity of infectious diseases. Coronavirus disease 2019 (COVID-19) is a potentially life-threatening viral infection that mainly infects the lungs. The objective of this review was to synthesize the existing findings from studies related to the effect of intravenous ascorbic acid on lung function in COVID-19 patients. For this review, PubMed, Cochrane, SCOPUS, EMBASE, Clinical Trial Registry, and Google Scholar databases were searched from December 2019 to May 2022. There was a total of six studies that investigated the large dose of ascorbic acid infusion intravenously on lung function in severely ill subjects with COVID-19. Out of six, three studies found that high-dose intravenous ascorbic acid improved lung function markers, and three studies found null results. Infusions of 12 g/d and 24 g/d of intravenous ascorbic acid had shown a significant improvement in lung function markers in two clinical trials. Studies that administered 8 g/d, 2 g/d, and 50 mg/kg/d of intravenous ascorbic acid found no influence on mechanical ventilation need and other lung function markers in critically ill subjects with COVID-19. Overall, the effect of intravenous ascorbic acid on the lung function of subjects with COVID yielded equivocal findings. More double-blinded, randomized, clinical studies with a larger sample size are required to confirm the effect of ascorbic acid in ameliorating the lung pathologies associated with COVID infection.
Influence of Machining Conditions on Micro-Geometric Accuracy Elements of Complex Helical Surfaces Generated by Thread Whirling
Authors: Merticaru Vasile , Nagîț Gheorghe , Dodun Oana , Merticaru Eugen , Rîpanu Marius Ionuț , Mihalache Andrei Marius , Slătineanu Laurențiu
Published: September 2022

Complex surfaces such as helical ones are commonly used in machinery. Such surfaces can be obtained by various machining processes, one of these processes being thread whirling. The influence of machining conditions needs to be better understood to develop a more precise prediction of the specific resulting errors involved in thread whirling. This paper firstly presents the theoretical conditions which generate micro-deviations on whirled surfaces. A theoretical model which considers the geometrical parameters describing the whirling head and cutters and the process’s whole kinematics was developed. The threaded surface was described as a complex compound surface resulting from intersecting successive ruled helical surfaces corresponding to the cutting edges of the set of cutters from the whirling head. Numerical simulation results were exemplified and validation experiments were both designed and performed. Empirical mathematical models were established to highlight the influence of the input factors such as thread pitch and external diameter, the ratio between the diameter of cutters’ top edge disposal and the thread’s external diameter, the rotary speed of the whirling head, and the rotary speed of the workpiece on some accuracy elements and roughness parameters of the threaded surface.
Antimicrobial Resistance and Increased Virulence of Salmonella
Authors: Khajanchi Bijay K. , Foley Steven L.
Published: September 2022

This special issue of Microorganisms highlights the importance of antimicrobial resistance (AMR) and increased virulence of Salmonella with multiple research papers [...]
The Resemblance between Bacterial Gut Colonization in Pigs and Humans
Authors: Van de Vliet Michiel , Joossens Marie
Published: September 2022

Thorough understanding of the initial colonization process of human intestines is important to optimize the prevention of microbiota-associated diseases, and also to further improve the current microbial therapies. In recent years, therefore, colonization of the human gut has gained renewed interest. However, due to a lack of standardization of life events that might influence this early colonization process in humans, many generally accepted insights are based on deduction and assumption. In our review, we compare knowledge on colonization in humans with research in piglets, because the intestinal tract of pigs is remarkably similar to that of humans and the early-life events are more standardized. We assess potential similarities and challenge some concepts that have been widely accepted in human microbiota research. Bacterial colonization of the human gut is characterized by successive waves in a progressive process, to a complex gut microbiota community. After re-analyzing available data from piglets, we found that the bacterial colonization process is very similar in terms of the wave sequence and functionality of each wave. Moreover, based on the piglet data, we found that, in addition to external factors such as suckling and nutrition, the bacterial community itself appears to have a major influence on the colonization success of additional bacteria in the intestine. Thus, the colonization process in piglets might rely, at least in part, on niche dependency, an ecological principle to be considered in the intestinal colonization process in humans.
Gut Bacteria and Neurotransmitters
Authors: Dicks Leon M. T.
Published: September 2022

Gut bacteria play an important role in the digestion of food, immune activation, and regulation of entero-endocrine signaling pathways, but also communicate with the central nervous system (CNS) through the production of specific metabolic compounds, e.g., bile acids, short-chain fatty acids (SCFAs), glutamate (Glu), γ-aminobutyric acid (GABA), dopamine (DA), norepinephrine (NE), serotonin (5-HT) and histamine. Afferent vagus nerve (VN) fibers that transport signals from the gastro-intestinal tract (GIT) and gut microbiota to the brain are also linked to receptors in the esophagus, liver, and pancreas. In response to these stimuli, the brain sends signals back to entero-epithelial cells via efferent VN fibers. Fibers of the VN are not in direct contact with the gut wall or intestinal microbiota. Instead, signals reach the gut microbiota via 100 to 500 million neurons from the enteric nervous system (ENS) in the submucosa and myenteric plexus of the gut wall. The modulation, development, and renewal of ENS neurons are controlled by gut microbiota, especially those with the ability to produce and metabolize hormones. Signals generated by the hypothalamus reach the pituitary and adrenal glands and communicate with entero-epithelial cells via the hypothalamic pituitary adrenal axis (HPA). SCFAs produced by gut bacteria adhere to free fatty acid receptors (FFARs) on the surface of intestinal epithelial cells (IECs) and interact with neurons or enter the circulatory system. Gut bacteria alter the synthesis and degradation of neurotransmitters. This review focuses on the effect that gut bacteria have on the production of neurotransmitters and vice versa.
Prevalence and Characterization of Shiga Toxin Producing Escherichia coli Isolated from Animal Feed in Croatia
Authors: Sokolovic Marijana , Šimpraga Borka , Amšel-Zelenika Tajana , Berendika Marija , Krstulović Fani
Published: September 2022

A survey on prevalence and number of Shiga toxin-producing Escherichia (E.) coli (STEC) in animal feed was carried out over a period of nine years in the Republic of Croatia. A total of 1688 feed samples were collected from feed factories and poultry farms. Analysis included two standard procedures: sample enrichment and (a) immunomagnetic separation and plating on two selective media; or (b) plating on two selective media. Confirmation of STEC included morphological examination, biochemical tests, serotyping, and polymerase chain reaction. Morphological and biochemical characterization revealed 629 E. coli strains. Further serological screening method revealed 78 STEC and EPEC serotypes, while only 27 strains were confirmed as STEC with PCR. All positive samples (1.6%) originated from poultry farms and contained combination of virulence genes: eaeA, stx1, and/or stx2. Since the presence of stx (especially stx2) and eae are identified as risk factors for development of severe diseases in humans, results of this survey indicate that avian sources of STEC infections might be one of those “undefined sources” of human illnesses. Further research is necessary for evaluation of risks posed by contaminated feed, poultry, and environment.
Late Cretaceous Activity Record of the Guangsan Fault—Insights from Zircon U-Pb and Apatite Fission-Track Thermochronology
Authors: Ding Ruxin , Chen Weihao , Soares Cleber , Hou Weisheng , Li Zilong , Li Yangshijia , Huang Rongli , Zou Heping
Published: September 2022

The timing of fault activity is a concern for geologists. This study used zircon U-Pb and apatite fission-track dating of fault breccia to determine the upper and lower limits for the time of faulting. The Guangsan fault in South China was taken as an example, and zircon U-Pb and apatite fission-track thermochronology were applied to the surrounding rock and fault breccia. The surrounding rock and fault breccia demonstrated 74.9–91.8 Ma and 73.9–93.5 Ma zircon U-Pb dates, respectively, indicating that the breccia formed after 73.9 Ma. They also demonstrated 71.6 ± 7.3 Ma and 85.9 ± 8.2–65.5 ± 6.5 Ma fission-track dates, implying that the fault breccia samples likely formed before ~70 Ma. Their thermal histories were highly consistent: both showed rapid cooling during 70–65 Ma and slow cooling during 65–0 Ma, implying that the fault was likely still active during 70–65 Ma, resulting in the rapid exhumation.
Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy
Authors: Alkhalde Ammar , Seferovic Hannah , Abri Ali , Simbrunner Alvana , Hinterdorfer Peter , Oh Yoo Jin
Published: September 2022

The environmental oxygen level plays a critical role in corneal crosslinking (CXL), a treatment method to increase corneal biomechanical stability. In this study, we introduce a new CXL method (Bubble-CXL), in which intracameral oxygen serves as an additional oxygen source during eye treatment. The efficiency of this new method was compared with the efficiency of the standard CXL method. Three fresh porcine eye pairs were included in this study. One eye of each pair was treated with standard CXL, whereas in the partner eye, intracameral oxygen was injected prior to CXL and removed at the end of the procedure. The Young’s modulus of each cornea was measured using atomic force microscopy. All analyzed corneas treated with intracameral oxygen showed significantly higher Young’s modulus and thus an increased stiffness compared to the cornea of the partner eye treated with the standard protocol. Using intracameral oxygen in CXL therapy may increase crosslinking efficiency and improve biomechanical corneal properties.
Multi-Band High-Efficiency Multi-Functional Polarization Controller Based on Terahertz Metasurface
Authors: Chen Huaijun , Zhao Wenxia , Gong Xuejian , Du Lianlian , Cao Yunshan , Zhai Shilong , Song Kun
Published: September 2022

Electromagnetic metasurfaces with excellent electromagnetic wave regulation properties are promising for designing high-performance polarization control devices, while the application prospect of electromagnetic metasurfaces is limited because of the current development situations of the complex structure, low conversion efficiency, and narrow working bandwidth. In this work, we design a type of reflective terahertz metasurface made of a simple structure that can achieve multiple polarization modulation with high efficiency. It is shown that the presented metasurface can realize ultra-broadband, cross-polarization conversion with the relative working bandwidth reaching 94% and a conversion efficiency of over 90%. In addition, the proposed metasurface can also efficiently accomplish different polarization conversion functions, such as linear-to-linear, linear-to-circular, or circular-to-linear polarization conversion in multiple frequency bands. Due to the excellent properties, the designed metasurface can be used as a high-efficiency multi-functional polarization modulation device, and it has important application value in terahertz imaging, communication, biological detection, and other fields.
Synthesis of Two Porous CdS Rods by Anion Exchange Method and Their Photocatalytic Properties
Authors: Wang Liwei , Meng Ming , Zheng Ruirui , Li Xiaoli , Yuan Honglei
Published: September 2022

Semiconductor materials with pore structure have excellent physicochemical properties for photocatalytic reactions. Here, the one-step vulcanization of Cd-based MOF solid rods was successfully developed to synthesize two kinds of CdS rods with pore structure: hollow rods (HRs) and mesoporous rods (MRs). Among the three catalysts, the CdS HRs showed the highest photocatalytic efficiency, which could remove about 96.0% of RhB in 30 min under visible light irradiation. The enhanced photocatalytic activity of CdS HRs benefits from its novel hollow structure, which enhances the visible light absorption capability and the separation efficiency of photogenerated electron–hole pairs. The successful synthesis of CdS HRs has guiding significance for the design and synthesis of other hollow structures with high photocatalytic activity.
Fabrication and Development of Binder-Free Mn–Fe–S Mixed Metal Sulfide Loaded Ni-Foam as Electrode for the Asymmetric Coin Cell Supercapacitor Device
Authors: Shin Jae Cheol , Yang Hee Kwon , Lee Jeong Seok , Lee Jong Hyuk , Kang Min Gyu , Kwon Ein
Published: September 2022

Currently, the fast growth and advancement in technologies demands promising supercapacitors, which urgently require a distinctive electrode material with unique structures and excellent electrochemical properties. Herein, binder-free manganese iron sulfide (Mn–Fe–S) nanostructures were deposited directly onto Ni-foam through a facile one-step electrodeposition route in potentiodynamic mode. The deposition cycles were varied to investigate the effect of surface morphologies on Mn–Fe–S. The optimized deposition cycles result in a fragmented porous nanofibrous structure, which was confirmed using Field Emission Scanning Electron Microscopy (FE−SEM). X-ray photoelectron spectroscopy (XPS) confirmed the presence of Mn, Fe, and S elements. The energy dispersive X-ray spectroscopy and elemental mapping revealed a good distribution of Mn, Fe, and S elements across the Ni-foam. The electrochemical performance confirms a high areal capacitance of 795.7 mF cm−2 with a 24 μWh cm−2 energy density calculated at a 2 mA cm−2 current density for porous fragmented nanofiber Mn–Fe–S electrodes. The enhancement in capacitance is due to diffusive-controlled behavior dominating the capacitator, as shown by the charge–storage kinetics. Moreover, the assembled asymmetric coin cell device exhibited superior electrochemical performance with an acceptable cyclic performance of 78.7% for up to 95,000 consecutive cycles.
Cysteine-Mediated Green Synthesis of Copper Sulphide Nanoparticles: Biocompatibility Studies and Characterization as Counter Electrodes
Authors: Saona Luis A. , Campo-Giraldo Jessica L. , Anziani-Ostuni Giovanna , Órdenes-Aenishanslins Nicolás , Venegas Felipe A. , Giordana María F. , Díaz Carlos , Isaacs Mauricio , Bravo Denisse , Pérez-Donoso José M.
Published: September 2022

A one-pot green method for aqueous synthesis of fluorescent copper sulphide nanoparticles (NPs) was developed. The reaction was carried out in borax–citrate buffer at physiological pH, 37 °C, aerobic conditions and using Cu (II) and the biological thiol cysteine. NPs exhibit green fluorescence with a peak at 520 nm when excited at 410 nm and an absorbance peak at 410 nm. A size between 8–12 nm was determined by dynamic light scattering and transmission electron microscopy. An interplanar atomic distance of (3.5 ± 0.1) Å and a hexagonal chalcocite crystalline structure (βCh) of Cu2S NPs were also determined (HR-TEM). Furthermore, FTIR analyses revealed a Cu-S bond and the presence of organic molecules on NPs. Regarding toxicity, fluorescent Cu2S NPs display high biocompatibility when tested in cell lines and bacterial strains. Electrocatalytic activity of Cu2S NPs as counter electrodes was evaluated, and the best value of charge transfer resistance (Rct) was obtained with FTO/Cu2S (four layers). Consequently, the performance of biomimetic Cu2S NPs as counter electrodes in photovoltaic devices constructed using different sensitizers (ruthenium dye or CdTe NPs) and electrolytes (S2−/Sn2− or I−/I3−) was successfully checked. Altogether, novel characteristics of copper sulfide NPs such as green, simple, and inexpensive production, spectroscopic properties, high biocompatibility, and particularly their electrochemical performance, validate its use in different biotechnological applications.
Gut Microbiota and Fear Processing in Women Affected by Obesity: An Exploratory Pilot Study
Authors: Scarpina Federica , Turroni Silvia , Mambrini Sara , Barone Monica , Cattaldo Stefania , Mai Stefania , Prina Elisa , Bastoni Ilaria , Cappelli Simone , Castelnuovo Gianluca , Brigidi Patrizia , Scacchi Massimo , Mauro Alessandro
Published: September 2022

The microbiota–gut–brain axis extends beyond visceral perception, influencing higher-order brain structures, and ultimately psychological functions, such as fear processing. In this exploratory pilot study, we attempted to provide novel experimental evidence of a relationship between gut microbiota composition and diversity, and fear-processing in obesity, through a behavioral approach. Women affected by obesity were enrolled and profiled for gut microbiota, through 16S rRNA amplicon sequencing. Moreover, we tested their ability to recognize facial fearful expressions through an implicit-facial-emotion-recognition task. Finally, a traditional self-report questionnaire was used to assess their temperamental traits. The participants exhibited an unbalanced gut microbiota profile, along with impaired recognition of fearful expressions. Interestingly, dysbiosis was more severe in those participants with altered behavioral performance, with a decrease in typically health-associated microbes, and an increase in the potential pathobiont, Collinsella. Moreover, Collinsella was related to a lower expression of the persistence temperamental trait, while a higher expression of the harm-avoidance temperament, related to fear-driven anxiety symptoms, was linked to Lactobacillus. Once confirmed, our findings could pave the way for the design of innovative microbiome-based strategies for the treatment of psychological and emotional difficulties by mitigating obesity-related consequences and behaviors.
Non-High-Density Lipoprotein Cholesterol and Cardiovascular Outcomes in Chronic Kidney Disease: Results from KNOW-CKD Study
Authors: Suh Sang Heon , Oh Tae Ryom , Choi Hong Sang , Kim Chang Seong , Bae Eun Hui , Ma Seong Kwon , Oh Kook-Hwan , Han Seung Hyeok , Kim Soo Wan
Published: September 2022

As non-high-density lipoprotein cholesterol (non-HDL-C) levels account for all atherogenic lipoproteins, serum non-HDL-C level has been suggested to be a marker for cardiovascular (CV) risk stratification. Therefore, to unveil the association of serum non-HDL-C levels with CV outcomes in patients with non-dialysis chronic kidney disease (ND-CKD), the patients at stages 1 to 5 (n = 2152) from the Korean Cohort Study for Outcomes in Patients with Chronic Kidney Disease (KNOW-CKD) were prospectively analyzed. The subjects were divided into quintiles by serum non-HDL-C level. The primary outcome was a composite of all-cause death or non-fatal CV events. The median duration of follow-up was 6.940 years. The analysis using the Cox proportional hazard model unveiled that the composite CV event was significantly increased in the 5th quintile (adjusted hazard ratio 2.162, 95% confidence interval 1.174 to 3.981), compared to that of the 3rd quintile. A fully adjusted cubic spline model depicted a non-linear, J-shaped association between non-HDL-C and the risk of a composite CV event. The association remained robust in a series of sensitivity analyses, including the analysis of a cause-specific hazard model. Subgroup analyses reveled that the association is not significantly altered by clinical conditions, including age, gender, body mass index, estimated glomerular filtration rate, and albuminuria. In conclusion, high serum non-HDL-C level increased the risk of adverse CV outcomes among the patients with ND-CKD. Further studies are warranted to define the optimal target range of non-HDL-C levels in this population.
Resveratrol and Some of Its Derivatives as Promising Prophylactic Treatments for Neonatal Hypoxia-Ischemia
Authors: Roumes Hélène , Goudeneche Pierre , Pellerin Luc , Bouzier-Sore Anne-Karine
Published: September 2022

Due to the rate of occurrence of neonatal hypoxia-ischemia, its neuronal sequelae, and the lack of effective therapies, the development of new neuroprotective strategies is required. Polyphenols (including resveratrol) are molecules whose anti-apoptotic, anti-inflammatory, and anti-oxidative properties could be effective against the damage induced by neonatal hypoxia-ischemia. In this review article, very recent data concerning the neuroprotective role of polyphenols and the mechanisms at play are detailed, including a boost in brain energy metabolism. The results obtained with innovative approaches, such as maternal supplementation at nutritional doses, suggest that polyphenols could be a promising prophylactic treatment for neonatal hypoxia-ischemia.
The Ambiguous Aspects of Oxygen
Authors: Napolitano Gaetana , Fasciolo Gianluca , Venditti Paola
Published: September 2022

For most living beings, oxygen is an essential molecule for survival, being the basis of biological oxidations, which satisfy most of the energy needs of aerobic organisms. Oxygen can also behave as a toxic agent posing a threat to the existence of living beings since it can give rise to reactive oxygen species (ROS) that can oxidise biological macromolecules, among which proteins and lipids are the preferred targets. Oxidative damage can induce cell, tissue, and organ dysfunction, which leads to severe body damage and even death. The survival of the aerobic organism depends on the development of an elaborate antioxidant defence system adapted to the normal level of atmospheric oxygen. The production of ROS in the aerobic organism can occur accidentally from exposure to pollutants or radiation, but occurs constantly during normal metabolic reactions. Cells have evolved using ROS to their advantage. Indeed, ROS are used as signalling molecules in numerous physiological processes, including muscle contraction, regulation of insulin release, and adaptation to environmental changes. Therefore, supplementation with antioxidants must be used wisely. A low level of ROS is essential for adaptation processes, so an excess of antioxidants can be harmful. Conversely, in conditions where ROS production increases, antioxidants can be useful to avoid cellular dysfunction.
Rabies Virus Seroprevalence among Dogs in Limpopo National Park and the Phylogenetic Analyses of Rabies Viruses in Mozambique
Authors: Mapatse Milton , Ngoepe Ernest , Abernethy Darrell , Fafetine José Manuel , Anahory Iolanda , Sabeta Claude
Published: September 2022

Rabies is considered a neglected disease among many developing Asian and African countries, including Mozambique, where its re-emergence is often attributed to low dog parenteral vaccination coverage. The objectives of this study were two-fold: (1) to assess the level of antibodies against rabies virus in dogs (n = 418) in Limpopo National Park (LNP), and (2) to genetically characterise selected rabies viruses from brain tissue samples collected in 2017 and 2018. To meet the first objective, we used the BioProTM Rabies blocking ELISA antibody kit, and the results were expressed as the percentage of blocking (%PB). Dog sera with PB ≥ 40% were considered positive for antibodies to rabies virus, whereas sera with PB < 40% were negative. Just under ninety percent (89.2%; n = 373) of dogs were seronegative, and the rest (10.8%; n = 45) had detectable levels of rabies virus-specific antibodies. All eight brain tissue samples were positive for rabies virus antigen using a direct fluorescent antibody test and amplified in a quantitative real-time PCR, but only five (n = 4 from dogs and n = 1 from a cat) were amplified in a conventional reverse-transcription PCR targeting partial regions of the nucleoprotein (N) and the glycoprotein (G) genes. All samples were successfully sequenced. Phylogenetically, the rabies viruses were all of dog origin and were very closely related to each other (Africa 1b rabies virus lineage). Furthermore, the sequences had a common progenitor with other rabies viruses from southern Africa, confirming the transboundary nature of rabies and the pivotal role of dogs in maintaining rabies cycles. The study demonstrates the principal application of the BioProTM rabies ELISA antibody for the detection of anti-lyssavirus-specific antibodies in the serum samples of dogs, and most importantly, it highlights the low levels of antibodies against rabies virus in this dog population.
Effects of Ceragenins and Antimicrobial Peptides on the A549 Cell Line and an In Vitro Co-Culture Model of A549 Cells and Pseudomonas aeruginosa
Authors: Oyardi Ozlem , Savage Paul B. , Guzel Cagla Bozkurt
Published: September 2022

Pseudomonas aeruginosa is an important pathogen that can adhere to host tissues and epithelial surfaces, especially during chronic infections such as cystic fibrosis (CF) lung infections. The effect of ceragenins and antimicrobial peptides (AMP) on this colonization was investigated in a co-culture infection model. After determining the antimicrobial effects of the substances on P. aeruginosa planktonic cells, their cytotoxicity on the A549 cell line was also determined. After the A549 cell line was infected with P. aeruginosa, the effect of antimicrobials on intracellular bacteria as well as the effects in inhibiting the adhesion of P. aeruginosa were investigated. In addition, LDH release from cells was determined by performing an LDH experiment to understand the cytotoxicity of bacterial infection and antimicrobial treatment on cells. CSA-131 was determined as the antimicrobial agent with the highest antimicrobial activity, while the antimicrobial effects of AMPs were found to be much lower than those of ceragenins. The antimicrobial with the lowest IC50 value was determined as the combination of CSA-131 with Pluronic F127. CSA-13 has been determined to be the most effective antimicrobial with its effectiveness to both intracellular bacteria and bacterial adhesion. Nevertheless, further safety, efficacy, toxicity, and pharmacological studies of ceragenins are needed to evaluate clinical utility.
Genetic Variants of Glucose-6-Phosphate Dehydrogenase and Their Associated Enzyme Activity: A Systematic Review and Meta-Analysis
Authors: Pfeffer Daniel A. , Satyagraha Ari Winasti , Sadhewa Arkasha , Alam Mohammad Shafiul , Bancone Germana , Boum Yap , Brito Marcelo , Cui Liwang , Deng Zeshuai , Domingo Gonzalo J. , He Yongshu , Khan Wasif A. , Kibria Mohammad Golam , Lacerda Marcus , Menard Didier , Monteiro Wuelton , Pal Sampa , Parikh Sunil , Roca-Feltrer Arantxa , Roh Michelle , Sirdah Mahmoud M. , Wang Duoquan , Huang Qiuying , Howes Rosalind E. , Price Ric N. , Ley Benedikt
Published: September 2022

Low glucose-6-phosphate dehydrogenase enzyme (G6PD) activity is a key determinant of drug-induced haemolysis. More than 230 clinically relevant genetic variants have been described. We investigated the variation in G6PD activity within and between different genetic variants. In this systematic review, individual patient data from studies reporting G6PD activity measured by spectrophotometry and corresponding the G6PD genotype were pooled (PROSPERO: CRD42020207448). G6PD activity was converted into percent normal activity applying study-specific definitions of 100%. In total, 4320 individuals from 17 studies across 10 countries were included, where 1738 (40.2%) had one of the 24 confirmed G6PD mutations, and 61 observations (3.5%) were identified as outliers. The median activity of the hemi-/homozygotes with A-(c.202G>A/c.376A>G) was 29.0% (range: 1.7% to 76.6%), 10.2% (range: 0.0% to 32.5%) for Mahidol, 16.9% (range 3.3% to 21.3%) for Mediterranean, 9.0% (range: 2.9% to 23.2%) for Vanua Lava, and 7.5% (range: 0.0% to 18.3%) for Viangchan. The median activity in heterozygotes was 72.1% (range: 16.4% to 127.1%) for A-(c.202G>A/c.376A>G), 54.5% (range: 0.0% to 112.8%) for Mahidol, 37.9% (range: 20.7% to 80.5%) for Mediterranean, 53.8% (range: 10.9% to 82.5%) for Vanua Lava, and 52.3% (range: 4.8% to 78.6%) for Viangchan. A total of 99.5% of hemi/homozygotes with the Mahidol mutation and 100% of those with the Mediterranean, Vanua Lava, and Viangchan mutations had <30% activity. For A-(c.202G>A/c.376A>G), 55% of hemi/homozygotes had <30% activity. The G6PD activity for each variant spanned the current classification thresholds used to define clinically relevant categories of enzymatic deficiency.
Role of Trientine in Hypertrophic Cardiomyopathy: A Review of Mechanistic Aspects
Authors: Ramli Fitri Fareez , Hashim Syed Alhafiz Syed , Raman Betty , Mahmod Masliza , Kamisah Yusof
Published: September 2022

Abnormality in myocardial copper homeostasis is believed to contribute to the development of cardiomyopathy. Trientine, a copper-chelating drug used in the management of patients with Wilson’s disease, demonstrates beneficial effects in patients with hypertrophic cardiomyopathy. This review aims to present the updated development of the roles of trientine in hypertrophic cardiomyopathy. The drug has been demonstrated in animal studies to restore myocardial intracellular copper content. However, its mechanisms for improving the medical condition remain unclear. Thus, comprehending its mechanistic aspects in cardiomyopathy is crucial and could help to expedite future research.
Quality of Active versus Spontaneous Reporting of Adverse Drug Reactions in Pediatric Patients: Relevance for Pharmacovigilance and Knowledge in Pediatric Medical Care
Authors: Dittrich Anne T. M. , Smeets Nori J. L. , de Jong Emma F. M. , Kämink Juliët L. , Kroeze Yvet , Draaisma Jos M. Th. , van Puijenbroek Eugène P. , te Loo D. Maroeska W. M.
Published: September 2022

For drug safety in pediatric patients, knowledge about adverse drug reactions (ADRs) is essential to balance benefits and risks, especially because of the high incidence of off-label drug use. However, underreporting of ADRs is a serious problem, leading to a deficit in knowledge affecting clinical practice. The aim of this study is to find a method by which we can improve the quantity of ADR reporting while maintaining or improving the quality of the ADR reports. This was done in several steps. First, health care providers were educated to increase awareness of ADRs. Thereafter, a novel active supporting system was introduced, where reporting ADRs was simplified; if clinical physicians suspected an ADR, they only had to send the name or hospital number of the patient, the observed ADR, and the suspected drug to a supportive team. This team collects all information needed about the possible ADR from the patient’s medical records and hospital charts. With this information, the supportive team fills in the forms necessary for reporting ADRs to the nationwide pharmacovigilance centre Lareb. With this system, the quantity of ADR reports from both inpatients and outpatients rose dramatically. Subsequently, the quality of the obtained ADR reports was measured using the ClinDoc and vigiGrade systems. This study shows there is no loss of quality of the ADR reports in the active reporting system compared to spontaneous reporting systems. Based on the data of the present study, we suggest that an active reporting system has the potential to increase our knowledge about ADRs in pediatric patients.
The Development and Clinical Applications of Oral Arsenic Trioxide for Acute Promyelocytic Leukaemia and Other Diseases
Authors: Chin Lynn , Kumana Cyrus R. , Kwong Yok-Lam , Gill Harinder
Published: September 2022

Appreciation of the properties of arsenic trioxide (ATO) has redefined the treatment landscape for acute promyelocytic leukaemia (APL) and offers promise as a treatment for numerous other diseases. The benefits of ATO in patients with APL is related to its ability to counteract the effects of PML::RARA, an oncoprotein that is invariably detected in the blood or bone marrow of affected individuals. The PML::RARA oncoprotein is degraded specifically by binding to ATO. Thus ATO, in combination with all-trans retinoic acid, has become the curative treatment for ATO. The multiple mechanisms of action of ATO has also paved the way for application in various condition encompassing autoimmune or inflammatory disorders, solid organ tumours, lymphomas and other subtypes of AML. The development of oral formulation of ATO (oral ATO) has reduced costs of treatment and improved treatment convenience allowing widespread applicability. In this review, we discuss the mechanisms of action of ATO, the development of oral ATO, and the applications of oral ATO in APL and other diseases.
Transport and Permeation Properties of Dapivirine: Understanding Potential Drug-Drug Interactions
Authors: Zheng Ruohui , Valicherla Guru R. , Zhang Junmei , Nuttall Jeremy , Silvera Peter , Marshall Leslie J. , Empey Philip E. , Rohan Lisa C.
Published: September 2022

The dapivirine (DPV) vaginal ring was developed by the nonprofit International Partnership for Microbicides (IPM) for reducing the risk of HIV infection. A clinical study (IPM 028) showed that concomitant use of the DPV ring and miconazole (MIC) altered DPV pharmacokinetic profile. In this work, we investigated whether or not DPV transport and permeation contributed to the observed DPV-MIC interaction. Our study evaluated the interaction between DPV and several transporters that are highly expressed in the human female reproductive tract, including MRP1, MRP4, P-gp, BCRP, and ENT1, using vesicular and cellular systems. We also evaluated the impact of DPV/MIC on cellular tight junctions by monitoring transepithelial electrical resistance with the Ussing chamber. Lastly, we evaluated the effect of MIC on DPV permeability across human cervical tissue. Our findings showed that DPV was not a substrate of MRP1, MRP4, P-gp, BCRP, or ENT1 transporters. Additionally, DPV did not inhibit the activity of these transporters. DPV, MIC, and their combination also did not disrupt cellular tight junctions. MIC did not affect DPV tissue permeability but significantly reduced DPV tissue levels. Therefore, our results suggest that the DPV-MIC interaction is not due to these five transporters, altered tight junction integrity, or altered tissue permeability.
Ring Resonator Gap Determination Design Rule and Parameter Extraction Method for Sub-GHz Resolution Whole C-Band Si3N4 Integrated Spectrometer
Authors: Hasan Gazi Mahamud , Liu Peng , Hasan Mehedi , Ghorbani Houman , Rad Mohammad , Bernier Eric , Hall Trevor J.
Published: September 2022

A panoramic ultra-high resolution photonic integrated circuit spectrometer is under development by the authors. The architecture comprises a tunable ring resonator (RR) stage and an AWG stage. The resolution defines the bandwidth of the RR, determined by the cross-coupled power and hence the gap between the access and ring waveguides. The AWG channel frequency spacing determines the required free-spectral range (FSR) and hence the perimeter of the ring resonator. The specified <1 GHz resolution combined with an FSR of 50 GHz renders accurate simulation difficult, obstructing the design process. In this report, a simplified design rule to determine the minimum gap between straight access waveguides and a circular ring waveguide is proposed. Realistic assumptions such as the existence of local bisymmetry and adiabatic mode evolution throughout the coupling region permit a simple mode solver to determine the relationship between the cross-coupled power and the minimum gap size. A parameter extraction method is also formulated for add-drop rings equipped with two nominally identical couplers that disentangles the loss and coupling ring parameters from intensity-only transmission measurements. The proposed rule is applied to the design of ring resonators fabricated on a Si3N4 platform. The parameter extraction method is used to analyze the measured characterization data of the ring resonators. The results show good agreement within ~43 nm between the design rule and the gaps size determined by the parameters extracted from the measured data and provide experimental confirmation of the technological viability of the ring resonators required by the spectrometer.
Genome-Wide Identification and Analysis of Lbd Transcription Factor Genes in Jatropha curcas and Related Species
Authors: Jin Qi , Yang Zitian , Yang Wenjing , Gao Xiaoyang , Liu Changning
Published: September 2022

Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors that play important roles in organ development and stress response. However, the function of LBD genes has not been reported in Euphorbiaceae. In this paper, we used Jatropha curcas as the main study object and added rubber tree (Hevea brasiliensis), cassava (Manihot esculenta Crantz) and castor (Ricinus communis L.) to take a phylogenetic analysis of LBD genes. Of LBD, 33, 58, 54 and 30 members were identified in J. curcas, rubber tree, cassava and castor, respectively. The phylogenetic analysis showed that LBD members of Euphorbiaceae could be classified into two major classes and seven subclasses (Ia-Ie,IIa-IIb), and LBD genes of Euphorbiaceae tended to cluster in the same branch. Further analysis showed that the LBD genes of Euphorbiaceae in the same clade usually had similar protein motifs and gene structures, and tissue expression patterns showed that they also have similar expression profiles. JcLBDs in class Ia and Ie are mainly expressed in male and female flowers, and there are multiple duplication genes with similar expression profiles in these clades. It was speculated that they are likely to play important regulatory roles in flower development. Our study provided a solid foundation for further investigation of the role of LBD genes in the sexual differentiaion of J. curcas.
Hybrid Nanofillers Creating the Stable PVDF Nanocomposite Films and Their Effect on the Friction and Mechanical Properties
Authors: Čech Barabaszová Karla , Holešová Sylva , Plesník Lukáš , Kolská Zdeňka , Joszko Kamil , Gzik-Zroska Bożena
Published: September 2022

The solvent casting method was used for five types of polyvinylidene difluoride (PVDF) nanocomposite film preparation. The effect of nanofillers in PVDF nanocomposite films on the structural, phase, and friction and mechanical properties was examined and compared with that of the natural PVDF film. The surface topography of PVDF nanocomposite films was investigated using a scanning electron microscope (SEM) and correlative imaging (CPEM, combinate AFM and SEM). A selection of 2D CPEM images was used for a detailed study of the spherulitic morphologies (grains size around 6–10 μm) and surface roughness (value of 50–68 nm). The chemical interactions were evaluated by Fourier transform infrared spectroscopy (FTIR). Dominant polar γ-phase in the original PVDF, PVDF_ZnO and PVDF_ZnO/V, the most stable non-polar α-phase in the PVDF_V_CH nanocomposite film and mixture of γ and α phases in the PVDF_V and PVDF_ZnO/V_CH nanocomposite films were confirmed. Moderately hydrophilic PVDF nanocomposite films with water contact angle values (WCA) in the range of 58°–69° showed surface stability with respect to the Zeta potential values. The effect of positive or negative Zeta-potential values of nanofillers (ζn) on the resulting negative Zeta-potential values (ζ) of PVDF nanocomposite films was demonstrated. Interaction of PVDF chains with hydroxy groups of vermiculite and amino and imino groups of CH caused transformation of γ-phase to α. The friction properties were evaluated based on the wear testing and mechanical properties were evaluated from the tensile tests based on Young’s modulus (E) and tensile strength (Rm) values. Used nanofillers caused decreasing of friction and mechanical properties of PVDF nanocomposite material films.
Preparation and Characteristics of Alginate Microparticles for Food, Pharmaceutical and Cosmetic Applications
Authors: Łętocha Anna , Miastkowska Małgorzata , Sikora Elżbieta
Published: September 2022

Alginates are the most widely used natural polymers in the pharmaceutical, food and cosmetic industries. Usually, they are applied as a thickening, gel-forming and stabilizing agent. Moreover, the alginate-based formulations such as matrices, membranes, nanospheres or microcapsules are often used as delivery systems. Alginate microparticles (AMP) are biocompatible, biodegradable and nontoxic carriers, applied to encapsulate hydrophilic active substances, including probiotics. Here, we report the methods most frequently used for AMP production and encapsulation of different actives. The technological parameters important in the process of AMP preparation, such as alginate concentration, the type and concentration of other reagents (cross-linking agents, oils, emulsifiers and pH regulators), agitation speed or cross-linking time, are reviewed. Furthermore, the advantages and disadvantages of alginate microparticles as delivery systems are discussed, and an overview of the active ingredients enclosed in the alginate carriers are presented.
Fabrication and Characterization of Flexible Solid Polymers Electrolytes for Supercapacitor Application
Authors: Badi Nacer , Theodore Azemtsop Manfo , Alghamdi Saleh A. , Al-Aoh Hatem A. , Lakhouit Abderrahim , Roy Aashis S. , Alatawi Ayshah S. , Ignatiev Alex
Published: September 2022

In this work, solid flexible polymer blend electrolytes (PBE) composed of polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) with different amounts of sodium thiocyanate (NaSCN) salt mixed in double-distilled water (solvent) are prepared via solution casting method. The obtained films are characterized using several techniques. The study of the surface morphology of the polymer blend salt complex films via the POM technique reveals the presence of amorphous regions due to the NaSCN effect. FTIR spectra studies confirm the complex formation between PVA, PVP, and NaSCN. The addition of 20 wt% NaSCN salt in the composition PVA: PVP (50:50 wt%) polymer blend matrix leads to an increase in the number of charge carriers and thus improves the ionic conductivity. The ionic conductivity of each polymer blend electrolyte was studied using the electrochemical impedance spectroscopy (EIS) method. The highest room temperature ionic conductivity of 8.1 × 10−5 S/cm S cm−1 is obtained for the composition of PVA: PVP (50:50 wt%) with 20 wt% NaSCN. LSV test shows the optimized ion-conducting polymer blend electrolyte is electrochemically stable up to 1.5 V. TNM analysis reveals that 99% of ions contribute for the conductivity against 1% of electrons only in the highly conductive polymer electrolyte PVA: PVP (50:50 wt%) + 20 wt% NaSCN. A supercapacitor device was fabricated using the optimized ion-conducting polymer blend film and graphene oxide (GO) coated electrodes. The GCD curve clearly reveals the behavior of an ideal capacitor with less Faradic process and low ESR value. The columbic efficiency of the GO-based system is found to be 100%, the GO-based electrode exhibits a specific capacitance of 12.15 F/g and the system delivers the charge for a long duration. The specific capacitance of the solid-state supercapacitor cell was found to be 13.28 F/g via the CV approach close to 14.25 F/g obtained with EIS data at low frequency.
Influence of Light-Intensity-Dependent Droplet Directionality on Dimensions of Structures Constructed Using an In Situ Light-Guided 3D Printing Method
Authors: Lim Jongkyeong , Lee Sangmin
Published: September 2022

As an alternative to conventional 3D printing methods that require supports, a new 3D printing strategy that utilizes guided light in situ has been developed for fabricating freestanding overhanging structures without supports. Light intensity has been found to be a crucial factor in modifying the dimensions of structures printed using this method; however, the underlying mechanism has not been clearly identified. Therefore, the light-intensity-dependent changes in the structure dimensions were analyzed in this study to elucidate the associated mechanism. Essentially, the entire process of deposition was monitored by assessing the behavior of photocurable droplets prior to their collision with the structure using imaging analysis tools such as a high-speed camera and MATLAB®. With increasing light intensity, the instability of the ejected falling droplets increased, and the droplet directionality deteriorated. This increased the dispersion of the droplet midpoints, which caused the average midpoints of the deposited single layers to shift further away from the center of the structure. Consequently, the diameter of the structure formed by successive stacking of single layers increased, and the layer thickness per droplet decreased. These led to light-intensity-dependent differences in the diameter and height of structures that were created from the same number of droplets.
Computational Approach to the Surface-Crosslinking Process of Superabsorbent Polymer via Central Composite Design
Authors: Kim Hae-Chan , Kwon Yong-Rok , Kim Jung-Soo , Kwon Miyeon , Kim Jong-Ho , Kim Dong-Hyun
Published: September 2022

The improvement of gel strength and absorption properties through the surface-crosslinking of superabsorbent polymers (SAPs) is essential for sanitary industry applications. We prepared core-SAP via aqueous solution copolymerization, and then surface-crosslinked the core-SAP under various conditions. The structure of the SAP was characterized using Fourier transform infrared (FT-IR) spectroscopy. Central composite design (CCD) of response surface methodology (RSM) has been applied to determine the optimum surface-crosslinking conditions such as surface-crosslinker content, reaction temperature, and reaction time. The optimal surface-crosslinking conditions were identified at a surface-crosslinker content of 2.22 mol%, reaction temperature of 160 °C, and reaction time of 8.7 min. The surface-crosslinked SAP showed excellent absorbency under load of 50 g/g with a permeability of 50 s. Other absorption properties were also evaluated by measuring the free absorbency and centrifuge retention capacity in saline solution.
Enhanced Antibacterial Performance of Chitosan/Corn Starch Films Containing TiO2/Graphene for Food Packaging
Authors: Liu Zhiyuan , Liu Xueqin
Published: September 2022

Chitosan (CTS)/corn starch (CSH)/nano-TiO2/graphene (Gr) antibacterial active packaging films were prepared by ultrasonic-assisted electrospray deposition and solution-casting methods, and the effects of the TiO2:Gr mass ratio and ultrasonication power on their morphology and mechanical, optical, thermal, barrier, and antibacterial properties were investigated. The film fabricated at a TiO2:Gr ratio of 6:4 and an ultrasonication power of 160 W exhibited a uniform distribution of the nanofillers in the CTS/CSH matrix and significantly enhanced the mechanical, barrier, and water-resistance properties. Furthermore, this film demonstrated superior ultraviolet and visible light-shielding characteristics as compared with those of the non-filled CTS/CSH film, while its Escherichia coli and Staphylococcus aureus inhibition efficiencies were equal to 96.67 ± 0.09% and 99.85 ± 0.13%, respectively. Therefore, the film can effectively prevent food spoilage, indicating its potential for food-packaging applications.