bluuebunny/crossref_metadata_2025 huggingface dataset:
Aerodynamic Analysis of Pressure Wave of High-Speed Maglev Vehicle Crossing: Modeling and Calculation
Authors: Gao Dinggang , Ni Fei , Lin Guobin , Luo Shihui , Ji Wen
Published: October 2019

When two maglev trains travel in opposite directions on two adjacent tracks, train crossing is inevitable. Especially when both trains run at full speed, the pressure wave formed by each other will have a significant impact on the structure of the vehicle. Therefore, it is important to understand the pressure distribution on the body surface during the crossing to mitigate impact of the pressure wave. In this work, numerical simulation techniques are employed to reveal the nature of pressure wave during train crossing. Firstly, the aerodynamic load calculation model and the pressure wave calculation model are established, based on the turbulence model and flow field control equation. Secondly, the governing equations are discretized together with determined corresponding boundary conditions, which leads to an effective numerical analysis method. Finally, the corresponding aerodynamic analysis is carried out for the high-speed maglev test vehicle running at speed 500 km/h on the open-air line. The simulation results reveal that the spot which sustains the most pressure fluctuation is at the widest part of the vehicle during the train crossing. This forms valuable insights on the aerodynamic nature of high-speed maglev train and provides necessary inputs to the structural design of the vehicle.
Improving Mountain Pine Beetle Survival Predictions Using Multi-Year Temperatures Across the Western USA
Authors: Bone Christopher , Nelson Michael France
Published: October 2019

Global climate change has led to an increase in large-scale bark beetle outbreaks in forests around the world, resulting in significant impacts to forest ecosystems, timber economies, and forest-dependent communities. As such, prediction models that utilize temperature for estimating future bark beetle locations and consequential tree mortality are critical for informing forest management decision-making in an attempt to mitigate and adapt to pending and current outbreaks. This is especially true for physiological models that account for the effects of overwinter temperatures on bark beetle survival, as seasonal temperatures, specifically during winter months, exert the greatest impact on bark beetle mortality during various stages of life cycle development. Yet, how temperature observations are used to predict bark beetle survival can significantly under- or over-estimate the role that temperature variability plays in annual tree mortality, especially under current climate change trajectories. This study evaluates how representations of winter temperature influence bark beetle survival estimates. Using the recent outbreak of mountain pine beetle (Dendroctonus ponderosae Hopkins) across the western USA as a case study, single-year to decade-long winter temperature averages were used as inputs into a physiological beetle survival prediction model, the results of which were compared against beetle-induced tree mortality observations using temporal autoregressive models. Results show that using longer-term survival averages of seven to ten years significantly increases the likelihood that temperature alone can predict general levels of beetle survival and hence beetle-induced mortality. These findings demonstrate the importance of considering the role of long-term temperature observations when forecasting bark beetle outbreaks, and that year-to-year temperature variability may be constrained in predicting beetle survival during outbreak periods.
Community Attributes Predict the Relationship between Habitat Invasibility and Land Use Types in an Agricultural and Forest Landscape
Authors: Zhou Yi , Su Yuqiao , Zhong Yonglin , Xie Peiyun , Xu Mingfeng , Su Zhiyao
Published: October 2019

Finding ecosystem or community level indicators for habitat invasibility may provide natural resource managers with environmentally friendly measures to control alien plant invasion; yet, ecosystem invasibility remains understudied. Here, we investigated alien plant invasion into various ecosystems representing different land use types in a subtropical peri-urban area of south China. Four invasive alien species were found from five out of the six ecosystems. Lower plant diversity in both the overstory and understory was consistently associated with more severe alien plant invasion to the ecosystems. The highest total abundance and plot occurrence of the invasive plants were found in the agroforestry ecosystem representing the highest disturbance. At plot scale, an increase in invasion severity was associated with a significant decrease in overstory stem density, species richness, and diversity, but with a significant increase in overstory plant dominance. The understory community attributes in response to the increase in invasion severity followed similar patterns, except that the stem density increased with invasion severity. Higher canopy openness and thus lower leaf area index and greater understory radiation were associated with higher invasion severity of invasive plants to the understory habitat. For predicting total abundance of the invasive species, the most important variable is land use type, while for the abundance of Lantana camara and Mikania micrantha, the most important predictor variable is overstory Berger–Parker index and canopy openness, respectively. Canopy structure and understory gap light regimes were among the most important factors determining the abundance of the worst invasive plant Mikania micrantha. Our results demonstrate that land use types with varying disturbance regimes determine the spatial heterogeneity in plant diversity and community structure, which predicts alien plant invasion and habitat invasibility; and that the severity of alien plant invasion in turn is a good indicator of habitat disturbance across the ecosystems.
The Bioenergetic Potential of Four Oak Species from Northeastern Mexico
Authors: Núñez-Retana Víctor Daniel , Wehenkel Christian , Vega-Nieva Daniel José , García-Quezada Juan , Carrillo-Parra Artemio
Published: October 2019

Lack of knowledge regarding the fuel quality of diverse tree species prevents their use. Furthermore, the potential use of wood with the bark of different tree species for pellet production is still relatively unexplored in the scientific literature. In Mexico, the sawdust of Quercus genus (oak) is underutilized, despite it being an important forest resource, due to some anatomical and technological characteristics. The sawdust of Quercus with bark is also considered to have a low economic value. The objective of this study was to analyze the energy characteristics of barked and debarked Quercus sideroxyla, Q. rugosa, Q. laeta, and Q. conzattii in order to evaluate their potential for pellet production. Granulometric distribution, bulk density, proximal analysis, and calorific value tests were carried out. The sawdust of the four tree species studied was in accordance with the limits established by the standard EN 14961-2. Sawdust with a particle size of 0.425 mm had the highest percentage of retained mass (30.33%) (p < 0.05) in the granulometry test. There were no statistical differences in granulometry (p > 0.05) between barked and debarked sawdust for all Quercus species. Barked sawdust presented higher bulk density (p < 0.05) than debarked sawdust (246 and 224 kg/m3, respectively). The moisture content did not show statistical differences (p > 0.05) between barked and debarked sawdust. The volatile material was higher (p < 0.05) in debarked sawdust (88.7%) than in barked sawdust (85.0%). The ash content was below 0.5%. The fixed carbon was higher (p < 0.05) in barked sawdust (14.6%). The calorific value was higher (p < 0.05) in barked sawdust and for the Q. rugosa species (19.5 MJ/kg). The results suggest that the oak species analyzed, both barked and debarked, showed good potential for pellet production. Future studies should quantify fuel quality for a variety of diameter distributions, and analyze pellet mechanical properties and ash slagging risk.
Landfill Impacts on the Environment—Review
Authors: Vaverková Magdalena Daria
Published: October 2019

Waste management (WM) is a demanding undertaking in all countries, with important implications for human health, environmental preservation, sustainability and circular economy. The method of sanitary landfilling for final disposal of waste remains a generally accepted and used method but the available scientific evidence on the waste-related environmental and health effects is not conclusive. Comparative studies of various WM methods (landfilling, incineration, composting etc.) show that among the municipal solid waste (MSW) treatment and disposal technological options, sanitary landfilling or open dumping is popular in most countries because of the relative low cost and low-technical requirement. The European Union (EU) Directive on waste landfills has introduced specific goals for reducing the volume of disposed waste and very strict requirements for landfilling and landfill sites. Evaluation of the impact of landfills on the environment is a crucial topic in the literature and has received increased attention recently, given growing environmental concerns. The main goal of this survey was to conduct a comprehensive assessment of possible impacts of MSW landfills on the environment. The main conclusion of the overall assessment of the literature is that the disposal of MSW in landfills entails a number of environmental risks but with respect to the current situation and rich style of living adopted in industrially developed countries, the idea of WM systems functioning without landfilling—at least in the foreseeable future within one generation—seems to be somewhat unreal. The results also provided important information of landfills as a source of environmental risk. Results of this research may have an important impact on landfill management and the disposal of waste. From the literature review, it is evident that even if high levels of waste avoidance, reuse and recycling are achieved, some waste materials will always need to be forwarded for disposal.
Doing Hydrology Backwards—Analytic Solution Connecting Streamflow Oscillations at the Basin Outlet to Average Evaporation on a Hillslope
Authors: Fonley Morgan , Mantilla Ricardo , Curtu Rodica
Published: October 2019

The concept of doing hydrology backwards, introduced in the literature in the last decade, relies on the possibility to invert the equations relating streamflow fluctuations at the catchment outlet to estimated hydrological forcings throughout the basin. In this work, we use a recently developed set of equations connecting streamflow oscillations at the catchment outlet to baseflow oscillations at the hillslope scale. The hillslope-scale oscillations are then used to infer the pattern of evaporation needed for streamflow oscillations to occur. The inversion is illustrated using two conceptual models of movement of water in the subsurface with different levels of complexity, but still simple enough to demonstrate our approach. Our work is limited to environments where diel oscillations in streamflow are a strong signal in streamflow data. We demonstrate our methodology by applying it to data collected in the Dry Creek Experimental Watershed in Idaho and show that the hydrology backwards principles yield results that are well within the order of magnitude of daily evapotranspiration fluctuations. Our analytic results are generic and they encourage the development of experimental campaigns to validate integrated hydrological models and test implicit parameterization assumptions.
Sustainable Environmental Strategies for Shrinking Cities Based on Processing Successful Case Studies Facing Decline Using a Decision-Support System
Authors: Campos-Sánchez Francisco , Reinoso-Bellido Rafael , Abarca-Álvarez Francisco
Published: October 2019

Since the middle of the last century post-industrial cities around the world have been losing population and shrinking due to the decline of their structural growth models, showing important socioeconomic transformations. This is a negative phenomenon but one that cities can benefit from. The aim of this work is to verify what type of measures against urban decline would be most suitable if applied to a specific case study. To do this, international cases of shrinking cities where successful measures were already carried out facing decline: (i) are collected, (ii) are classified based on several influencing criteria, and (iii) are grouped under similar alternatives against the decline. Measures and criteria focused on achieving sustainability are emphasized. Alternatives are then prioritised using an Analytic Hierarchy Process designed at several hierarchical levels. The results are discussed based on the construction of sustainable future scenarios according to the optimal alternatives regarding the case study, improving the model validity. The work evidences that environmental and low-cost measures encouraging the economy and increasing the quality of life, regardless of the city size-population range where they were performed, may be the most replicable. Future research lines on the integration of the method together with other decision-support systems and techniques are provided.
Availability, Price and Affordability of Anticancer Medicines: Evidence from Two Cross-Sectional Surveys in the Jiangsu Province, China
Authors: Zhu Yulei , Wang Ying , Sun Xiaoluan , Li Xin
Published: October 2019

Objectives: With the increasing incidence of cancer, poor access to affordable anticancer medicines has been a serious public health problem in China. To help address this issue, we assessed the availability, price and affordability of pharmacotherapy for cancer in public hospitals in the Jiangsu Province, China. Methods: In 2012 and 2016, anticancer medicine availability and price information in the capital and five other cities was collected. A total of six cancer care hospitals, 26 tertiary general hospitals and 28 secondary general hospitals were sampled, using an adaptation of the World Health Organization/Health Action International methodology. Data was collected for the anticancer medicines in stock at the time of the surveys. Prices were expressed as inflation-adjusted median unit prices (MUPs). Medicine was affordable if the overall cost of all the prescribed anticancer medicines was less than 20% of the household’s capacity to pay. We used generalized estimating equations to estimate the significance of differences in availability from 2012 to 2016 and the Wilcoxon rank test to estimate the significance of differences in MUPs. Multivariate logistic regression was computed to measure predictors of affordability. Results: From 2012 to 2016 there was a significant decrease in the mean availability of originator brands (OBs) (from 7.79% to 5.71%, p = 0.012) and lowest-priced generics (LPGs) (36.29% to 32.67%, p = 0.009). The mean availability of anticancer medicines in secondary general hospitals was significantly lower than the cancer care, as well as in tertiary general hospitals. The MUPs of OBs (difference: −21.29%, p < 0.01) and their LPGs (−22.63%, p < 0.01) decreased significantly from 2012 to 2016. The OBs (16.67%) of all the anticancer medicines were found to be less affordable than LPGs (34.62% for urban residents and 30.77% for rural residents); their affordability varied among the different income regions. From 2012 to 2016, the proportion of LPGs with low availability and low affordability dropped from 30.77% to 19.23% in urban areas and 34.62% to 26.92% in rural areas, respectively. Generic substitution and medicine covered by basic medical insurance are factors facilitating affordability. Conclusion: There were concerning decreases in the availability of anticancer medicines in 2016 from already low availability in 2012. Anticancer medicines were more affordable for the patients in high-income regions than the patients in low-income regions. Governments should consider using their bargaining power to reduce procurement prices and abolish taxes on anticancer medicines. Policy should focus on the special health insurance plan for low-income patients with cancer. The goal of drug policy should ensure that first-line generic drugs are available for cancer patients and preferentially prescribed.
Identifying Risk Profiles of School Refusal Behavior: Differences in Social Anxiety and Family Functioning Among Spanish Adolescents
Authors: Gonzálvez Carolina , Díaz-Herrero Ángela , Sanmartín Ricardo , Vicent María , Pérez-Sánchez Antonio M. , García-Fernández José M.
Published: October 2019

School attendance problems negatively affect students’ development. This study attempted to identify different school refusal behavior profiles and to examine their relationship with three dimensions of social anxiety (fear of negative evaluation, social avoidance and distress in new situations, and social avoidance and distress that is experienced more generally in the company of peers) and the perception of family functioning. Participants included 1842 Spanish adolescents (53% girls) aged 15–18 years (M = 16.43; SD = 1.05). The School Refusal Assessment Scale—Revised (SRAS-R), the Social Anxiety Scale for Adolescents (SAS-A), and the Family APGAR Scale (APGAR: Adaptation, Partnership, Growth, Affection, and Resolve) were administered. Latent class analysis revealed four school refusal behavior profiles: non-school refusal behavior, high school refusal behavior, moderately low school refusal behavior, and moderately high school refusal behavior. Analyses of variance (ANOVA) indicated that adolescents’ with the profile of high school refusal behavior showed higher scores in all the subscales of social anxiety. In contrast, the non-school refusal behavior group revealed higher scores in the perception of good family functioning, whereas the high school refusal behavior profile obtained the lowest scores in this scale. These findings suggest that students who reject school are at a higher risk of developing social anxiety problems and manifesting family conflicts. These students should be prioritized in order to attend to their needs, promoting self-help to overcome social anxiety and family problems with the purpose of preventing school refusal behaviors.
Relationship between Air Pollution and Regional Longevity in Guangxi, China
Authors: Deng Qucheng , Wei Yongping , Chen Lijuan , Liang Wei , Du Jijun , Tan Yuling , Zhao Yinjun
Published: October 2019

Air pollution has become a global environmental challenge and poses major threats to human health, particularly for the aging population. However, few studies have investigated the effects of air pollutants on human longevity, especially based on the total regional quantities and sources. Based on investigation of the spatiotemporal variations of three air pollutants (PM10, SO2, and NOx) and three longevity indicators (centenarian ratio, centenarity index, and aging tendency), this study aims to identify the relationship between air pollution and regional longevity in Guangxi Province. Air pollutant and population data from 109 counties and areas of Guangxi were collected from environmental research reports and statistical yearbooks. Cluster and outlier analysis was used to detect the regions with high and low clusters of the longevity indicators and air pollutants. Geographically weighted regression analyses were performed to determine the relationship between longevity and air pollutants. A negative relationship between the air pollutants PM10, SO2, and NOx on the aged population was observed. From a provincial level, industrial sources from the urban areas of cities located in the central province, including Liuzhou, Nanning, Laibing, Guigang and Yulin, were important contributors to the air pollutants PM10, SO2, and NOx, and thus could contribute to negative impacts on regional longevity. The key findings from this study will provide a case for management of air pollutants based on public health policies in China as well as other developing communities.
Pre-Pregnancy BMI Influences the Association of Dietary Quality and Gestational Weight Gain: The SECOST Study
Authors: Yong Heng Yaw , Mohd Shariff Zalilah , Mohd Yusof Barakatun Nisak , Rejali Zulida , Tee Yvonne Yee Siang , Bindels Jacques , van der Beek Eline M.
Published: October 2019

Poor diet quality in pregnancy could impact gestational weight gain (GWG) and consequently fetal growth and development. But today there is limited data available on gestational diet quality. This study investigated the association between diet quality in each pregnancy trimester and GWG in Malaysian women. Diet quality was assessed using the modified Healthy Eating Index for Malaysians (HEI). Total GWG was defined as the difference between measured weight at last prenatal visit and pre-pregnancy weight. About one-fourth of women (23.3%) had excessive total GWG. There were significant differences in the HEI component score across trimesters, except for fruits. Overall, overweight/obese women had lower total HEI score (51.49–55.40) during pregnancy compared to non-overweight/obese women (53.38–56.50). For non-overweight/obese women, higher total HEI scores in the second and third trimesters were significantly associated with lower risk of inadequate GWG (aOR = 0.97, 95% CI = 0.95–0.99, p = 0.01) and higher risk of excessive GWG (aOR = 1.04, 95% CI = 1.01–1.07, p = 0.03), respectively. Overweight/obese women with higher total HEI scores in the second (aOR = 1.04, 95% CI = 1.01–1.07, p = 0.02) and third trimester (aOR = 1.04, 95% CI = 1.01–1.08, p = 0.02) were significantly at higher risk for excessive GWG. Pregnant women had relatively low diet quality throughout pregnancy. Diet quality and GWG association differed according to pre-pregnancy BMI with excessive GWG more likely to be associated with higher total HEI scores in the third trimester.
Airborne Rodent Allergen Levels in Dutch Households: A Pilot Study
Authors: Burt Sara A. , Parramon Dolcet Lidia I. , Wouters Inge M.
Published: October 2019

Little research has been conducted in Europe regarding indoor exposure to airborne rodent allergens. The aims of this study were to gain insight into the prevalence of rodent allergens in airborne dust in Dutch households, to assess whether there is a relationship between rodent sightings and detectable allergens, and to identify risk factors associated with the presence of rodent allergens. Airborne dust was collected from the living rooms of 80 households distributed around central Netherlands by passive sampling using electrostatic dust collectors (EDCs). Samples were analysed for mouse (Mus m 1) and rat (Rat n 1) allergens. Participants completed a questionnaire on household and building characteristics, household pets, cleaning habits and ventilation. Mouse allergen was more prevalent than rat allergen and mouse sightings within the past year more than doubled the odds of detectable mouse allergen. Proximity to green areas, ventilation through an open window and insulation under the living room floor were determinants for detectable mouse allergen. Conversely, proximity to surface water was protective. No significant association was found between asthma and detectable mouse allergen. The passive EDC sampling method was used successfully to detect mouse and rat allergens in homes. The presence of mouse allergen was associated with previous mouse sightings. Risk factors and protective factors associated with the presence and levels of mouse allergen were identified.
“Let’s Talk About It”: The Moderating Role of Self-Disclosure on Complicated Grief over Time among Suicide Survivors
Authors: Levi-Belz Yossi , Lev-Ari Lilac
Published: October 2019

Suicide often imparts highly stressful ramifications to those left behind. Previous research on suicide survivors (SUSs) has demonstrated their being at high risk for developing anxiety and depression, including pathological complicated grief (CG). Self-disclosure (S-D)––the tendency to share one’s personal feelings––has been found to be an important component of dealing with grief. In this study, we examined the effect of S-D on CG in an 18-month longitudinal design following one hundred fifty-six SUSs. We found that SUSs suffering from pathological CG at Time 1 (T1) were lower in S-D at T1 and T2 and higher in depression at T2. We also found that SUSs with lower S-D at T1 had higher CG at T2. Using a structural equation model, we found that S-D at T1 contributed significantly (and negatively) to CG at T1, above and beyond the natural fading of CG over time. Our findings emphasize that while CG is highly prevalent among SUSs, S-D has a beneficial effect which can serve as a protective factor against CG for this group. Implications regarding possible interventions with SUSs were discussed.
Spatial Distribution and Health Risk Assessment of Potentially Toxic Elements in Surface Soils of Bosten Lake Basin, Central Asia
Authors: Ma Long , Abuduwaili Jilili , Liu Wen
Published: October 2019

A geographically weighted regression and classical linear model were applied to quantitatively reveal the factors influencing the spatial distribution of potentially toxic elements of forty-eight surface soils from Bosten Lake basin in Central Asia. At the basin scale, the spatial distribution of the majority of potentially toxic elements, including: cobalt (Co), chromium (Cr), copper (Cu), nickel (Ni), lead (Pb), thallium (Tl), vanadium (V), and zinc (Zn), had been significantly influenced by the geochemical characteristics of the soil parent material. However, the arsenic (As), cadmium (Cd), antimony (Sb), and mercury (Hg) have been influenced by the total organic matter in soils. Compared with the results of the classical linear model, the geographically weighted regression can significantly increase the level of simulation at the basin spatial scale. The fitting coefficients of the predicted values and the actual measured values significantly increased from the classical linear model (Hg: r2 = 0.31; Sb: r2 = 0.64; Cd: r2 = 0.81; and As: r2 = 0.68) to the geographically weighted regression (Hg: r2 = 0.56; Sb: r2 = 0.74; Cd: r2 = 0.89; and As: r2 = 0.85). Based on the results of the geographically weighted regression, the average values of the total organic matter for As (28.7%), Cd (39.2%), Hg (46.5%), and Sb (26.6%) were higher than those for the other potentially toxic elements: Cr (0.1%), Co (4.0%), Ni (5.3%), V (0.7%), Cu (18.0%), Pb (7.8%), Tl (14.4%), and Zn (21.4%). There were no significant non-carcinogenic risks to human health, however, the results suggested that the spatial distribution of potentially toxic elements had significant differences.
Mapping the Celebrity Endorsement of Branded Food and Beverage Products and Marketing Campaigns in the United States, 1990–2017
Authors: Zhou Mi , Rajamohan Srijith , Hedrick Valisa , Rincón-Gallardo Patiño Sofia , Abidi Faiz , Polys Nicholas , Kraak Vivica
Published: October 2019

Celebrity endorsement used to promote energy-dense and nutrient-poor (EDNP) food and beverage products may contribute to poor dietary habits. This study examined celebrity endorsement of branded food and beverage products and marketing campaigns in the United States (US) from 1990 to 2017. Celebrity endorsement data were collected from peer-reviewed and grey literature. Interactive data visualizations were created for the endorsement relationships between celebrities, companies and products whose nutritional profiles were compared with the US Department of Agriculture’s (USDA’s) Smart Snacks Standards. Logistic regression was used to explore associations between celebrities’ demographic profiles and the nutritional profiles of products. Results showed 542 celebrities were associated with 732 endorsements representing 120 brands of 59 companies across 10 food and beverage categories. Two thirds (67.2%; n = 80) of the brands represented EDNP products that did not align with the USDA’s Smart Snacks Standards. Logistic regression analysis indicated that Millennial (p = 0.008) and male celebrities (p = 0.041) were more likely to endorse EDNP products than Generation Z teen and female celebrities, respectively. No statistical significance was observed for celebrities of other demographic profiles. This study may inform future policies and actions of the US government, industry, researchers and consumer advocacy organizations to use celebrity endorsement to promote healthy food environments for Americans.
Health Implications of Drinking Water Salinity in Coastal Areas of Bangladesh
Authors: Chakraborty Rishika , Khan Khalid M. , Dibaba Daniel T. , Khan Md Alfazal , Ahmed Ali , Islam Mohammad Zahirul
Published: October 2019

Coastal areas in South Asian countries are particularly vulnerable to elevated water salinity. Drinking water salinity has been found to be associated with cardiovascular diseases (CVD), diarrhea, and abdominal pain. Our study aimed to find if excess drinking water salinity was associated with increased hospital visits with an array of health effects in coastal sub-districts of Bangladesh. A cross-sectional study was conducted with 157 participants from three coastal sub-districts. A face-to-face interview was conducted to collect salinity exposure and hospital visit data. Water samples were collected from both drinking and other household water sources for the measurement of salinity and total dissolved solids (TDS). CVD, diarrhea, and abdominal pain related hospital visits were found to be significantly associated with high water salinity and TDS. Households exposed to high salinity demonstrated a higher frequency of hospital visits than the low salinity-exposed households. People exposed to high salinity seemed to lack awareness regarding salinity-inducing health effects. Water salinity is a public health concern that will continue to rise due to climate change. Therefore, raising awareness about the health risks of water salinity is essential for the government to frame policies and mitigation strategies to control this emerging threat.
Do Older Adults with Multimorbidity Meet the Recommended Levels of Physical Activity? An Analysis of Scottish Health Survey
Authors: Salman Ahmad , Sellami Maha
Published: October 2019

There is a positive association between physical activity (PA) and improved health in older adults. The objective of this study was to assess the prevalence and determinants of meeting recommended levels of PA among older adults with multimorbidity. Data has been derived from the nationally representative Scottish Health Surveys (2014–2017). A sub-sample of 2230 older adults (aged 65+) with multimorbidity were the study participants. Physical activity was evaluated using current recommended guidelines. Overall, 32.3% of the participants met the recommended levels of PA. Independent predictors of meeting the recommended levels of PA include male gender [odds ratio (OR) 2.00 (95% confidence interval (CI) 1.58–2.54)], living in the least deprived areas [OR 1.79 (95% CI 1.20–2.69)]; being a non-smoker [OR 2.22 (95% CI 1.48–3. 34)]. Also, meeting recommended PA decreased with age [OR 0.92 (95% CI 0.90–0.94)] and body mass index [OR 0.93 (95% CI 0.91–0.95]; but increased per additional portion of fruit and vegetables taken [OR 1.19 (95% CI 1.12–1.25)] and with increase in well-being scale score [OR 1.05 (95% CI 1.03 to 1.06)]. Adherence to PA guidelines seems to be more related to age, BMI, gender (i.e. higher PA adherence in men vs. women), social support (i.e. social deprivation), dietary habits (i.e. fruit and vegetable intake) and social isolation among the elderly. In the one-third of older population, adherence to PA was associated to better mental health. Therefore, adaptation of PA guideline to suit theses determinants factors would reduce the gap difference among older adults with multimorbidity and enhance their mental well-being.
Feasibility Study of a Menstrual Hygiene Management Intervention for People with Intellectual Impairments and Their Carers in Nepal
Authors: Wilbur Jane , Mahon Thérèse , Torondel Belen , Hameed Shaffa , Kuper Hannah
Published: October 2019

Background: The Bishesta campaign is a menstrual hygiene management (MHM) intervention developed to meet the specific needs of people with intellectual impairments and their carers. It was designed and delivered in the Kavre district, Nepal. This paper explores the campaign’s feasibility and acceptability. Methods: The Bishesta campaign was delivered to ten people with an intellectual impairment and their eight carers. Data on the feasibility and acceptability of the intervention was collected through: Structured questionnaire to participants before and after the intervention, process monitoring data, post-intervention in-depth interviews with all carers, observation of people with intellectual impairments, key informant interviews with all facilitators and staff involved in the campaign, as well as ranking of the perceived appropriateness and acceptability of campaign components by carers and facilitators. Results: The Bishesta campaign was acceptable for the target groups, facilitators, and implementers. It was largely delivered with fidelity. Participants used most of the campaign components; these made the target behaviours attractive and enabled participants to carry them out with ease. There were improvements across all target behaviours. The focus of this study was feasibility, not limited-efficancy; however, indicative positive outcomes from this small sample were observed, such as an increase in young people’s levels of confidence, comfort, and autonomy during menstruation. Conclusion: Within the sample, the Bishesta campaign appears to be a feasible intervention to ensure that one of the groups most vulnerable to exclusion from MHM interventions is not left behind.
A Multi-Dimensional Analysis of El Niño on Twitter: Spatial, Social, Temporal, and Semantic Perspectives
Authors: Ye Xinyue , Wei Xuebin
Published: October 2019

Social media platforms have become a critical virtual community where people share information and discuss issues. Their capabilities for fast dissemination and massive participation have placed under scrutiny the way in which they influence people’s perceptions over time and space. This paper investigates how El Niño, an extreme recurring weather phenomenon, was discussed on Twitter in the United States from December 2015 to January 2016. A multiple-dimensional analysis, including spatial, social, temporal, and semantic perspectives, is conducted to comprehensively understand Twitter users’ discussion of such weather phenomenon. We argue that such multi-dimensional analysis can reveal complicated patterns of Twitter users’ online discussion and answers questions that cannot be addressed with a single-dimension analysis. For example, a significant increase in tweets about El Niño was noted when a series of rainstorms inundated California in January 2016. Some discussions on natural disasters were influenced by their geographical distances to the disasters and the prevailing geopolitical environment. The popular tweets generally discussing El Niño were overall negative, while tweets talking about how to prepare for the California rainstorms were more positive.
Comparative Genome Analyses Reveal the Genomic Traits and Host Plant Adaptations of Flavobacterium akiainvivens IK-1T
Authors: Wan Xuehua
Published: October 2019

The genus Flavobacterium contains a large group of commensal bacteria identified in diverse terrestrial and aquatic habitats. We compared the genome of a new species Flavobacterium akiainvivens IK-1T to public available genomes of Flavobacterium species to reveal the genomic traits and ecological roles of IK-1T. Principle component analysis (PCA) of carbohydrate-active enzyme classes suggests that IK-1T belongs to a terrestrial clade of Flavobacterium. In addition, type 2 and type 9 secretion systems involved in bacteria-environment interactions were identified in the IK-1T genome. The IK-1T genome encodes eukaryotic-like domain containing proteins including ankyrin repeats, von Willebrand factor type A domain, and major royal jelly proteins, suggesting that IK-1T may alter plant host physiology by secreting eukaryotic-like proteins that mimic host proteins. A novel two-component system FaRpfC-FaYpdB was identified in the IK-1T genome, which may mediate quorum sensing to regulate global gene expressions. Our findings suggest that comparative genome analyses of Flavobacterium spp. reveal that IK-1T has adapted to a terrestrial niche. Further functional characterizations of IK-1T secreted proteins and their regulation systems will shed light on molecular basis of bacteria-plant interactions in environments.
Phytohormones (Auxin, Gibberellin) and ACC Deaminase In Vitro Synthesized by the Mycoparasitic Trichoderma DEMTkZ3A0 Strain and Changes in the Level of Auxin and Plant Resistance Markers in Wheat Seedlings Inoculated with this Strain Conidia
Authors: Jaroszuk-Ściseł Jolanta , Tyśkiewicz Renata , Nowak Artur , Ozimek Ewa , Majewska Małgorzata , Hanaka Agnieszka , Tyśkiewicz Katarzyna , Pawlik Anna , Janusz Grzegorz
Published: October 2019

Both hormonal balance and plant growth may be shaped by microorganisms synthesizing phytohormones, regulating its synthesis in the plant and inducing plant resistance by releasing elicitors from cell walls (CW) by degrading enzymes (CWDE). It was shown that the Trichoderma DEMTkZ3A0 strain, isolated from a healthy rye rhizosphere, colonized the rhizoplane of wheat seedlings and root border cells (RBC) and caused approximately 40% increase of stem weight. The strain inhibited (in over 90%) the growth of polyphagous Fusarium spp. (F. culmorum, F. oxysporum, F. graminearum) phytopathogens through a mechanism of mycoparasitism. Chitinolytic and glucanolytic activity, strongly stimulated by CW of F. culmorum in the DEMTkZ3A0 liquid culture, is most likely responsible for the lysis of hyphae and macroconidia of phytopathogenic Fusarium spp. as well as the release of plant resistance elicitors. In DEMTkZ3A0 inoculated plants, an increase in the activity of the six tested plant resistance markers and a decrease in the concentration of indoleacetic acid (IAA) auxin were noted. IAA and gibberellic acid (GA) but also the 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCD) enzyme regulating ethylene production by plant were synthesized by DEMTkZ3A0 in the liquid culture. IAA synthesis was dependent on tryptophan and negatively correlated with temperature, whereas GA synthesis was positively correlated with the biomass and temperature.
Plasma pS129-α-Synuclein Is a Surrogate Biofluid Marker of Motor Severity and Progression in Parkinson’s Disease
Authors: Lin Chin-Hsien , Liu Huei-Chun , Yang Shieh-Yueh , Yang Kai-Chien , Wu Chau-Chung , Chiu Ming-Jang
Published: October 2019

Phosphorylated α-synuclein accounts for more than 90% of α-synuclein found in Lewy bodies of Parkinson’s disease (PD). We aimed to examine whether plasma Ser129-phosphorylated α-synuclein (pS129-α-synuclein) is a surrogate marker of PD progression. This prospective study enrolled 170 participants (122 PD patients, 68 controls). We measured plasma levels of total and pS129-α-synuclein using immunomagnetic reduction-based immunoassay. PD patients received evaluations of motor and cognition at baseline and at a mean follow-up interval of three years. Changes in the Movement Disorder Society revision of the Unified Parkinson’s Disease Rating Scale motor score (MDS-UPDRS part III) and Mini-Mental State Examination (MMSE) score were used to assess motor and cognition progression. Our results showed that plasma levels of total and pS129-α-synuclein were significantly higher in PD patients than controls (total: 1302.3 ± 886.6 fg/mL vs. 77.8 ± 36.6 fg/mL, p < 0.001; pS129-α-synuclein: 12.9 ± 8.7 fg/mL vs. 0.8 ± 0.6 fg/mL, p < 0.001), as was the pS129-α-synuclein/total α-synuclein ratio (2.8 ± 1.1% vs. 1.1 ± 0.6%, p = 0.01). Among PD patients, pS129-α-synuclein levels were higher with advanced motor stage (p < 0.001) and correlated with MDS-UPDRS part III scores (r = 0.27, 95% CI: 0.09–0.43, p = 0.004). However, we found no remarkable difference between PD patients with and without dementia (p = 0.75). After a mean follow-up of 3.5 ± 2.1 years, PD patients with baseline pS129-α-synuclein > 8.5 fg/mL were at higher risk of motor symptom progression of at least 3 points in the MDS-UPDRS part III scores than those with pS129-α-synuclein < 8.5 fg/mL (p = 0.03, log rank test). In conclusion, our data suggest that plasma pS129-α-synuclein levels correlate with motor severity and progression, but not cognitive decline, in patients with PD.
Epithelial-Mesenchymal Transition-Related MicroRNAs and Their Target Genes in Colorectal Cancerogenesis
Authors: Ranković Branislava , Zidar Nina , Žlajpah Margareta , Boštjančič Emanuela
Published: October 2019

MicroRNAs of the miR-200 family have been shown experimentally to regulate epithelial-mesenchymal transition (EMT). Although EMT is the postulated mechanism of development and progression of colorectal cancer (CRC), there are still limited and controversial data on expression of miR-200 family and their target genes during CRC cancerogenesis. Our study included formalin-fixed paraffin-embedded biopsy samples of 40 patients (10 adenomas and 30 cases of CRC with corresponding normal mucosa). Expression of miR-141, miR-200a/b/c and miR-429 and their target genes (CDKN1B, ONECUT2, PTPN13, RND3, SOX2, TGFB2 and ZEB2) was analysed using quantitative real-time PCR. Expression of E-cadherin was analysed using immunohistochemistry. All miRNAs were down-regulated and their target genes showed the opposite expression in CRC compared to adenoma. Down-regulation of the miR-200 family at the invasive front in comparison to the central part of tumour was observed as well as a correlation of expression of miR-200b, CDKN1B, ONECUT2 and ZEB2 expression to nodal metastases. Expression of the miR-200 family and SOX2 also correlated with E-cadherin staining. These results suggest that the miR-200 family and their target genes contribute to progression of adenoma to CRC, invasive properties and development of metastases. Our results strongly support the postulated hypotheses of partial EMT and intra-tumour heterogeneity during CRC cancerogenesis.
The Genetics of Polycystic Ovary Syndrome: An Overview of Candidate Gene Systematic Reviews and Genome-Wide Association Studies
Authors: Hiam Danielle , Moreno-Asso Alba , Teede Helena J. , Laven Joop S.E. , Stepto Nigel K. , Moran Lisa J. , Gibson-Helm Melanie
Published: October 2019

Polycystic Ovary Syndrome (PCOS) is a complex condition with mechanisms likely to involve the interaction between genetics and lifestyle. Familial clustering of PCOS symptoms is well documented, providing evidence for a genetic contribution to the condition. This overview aims firstly to systematically summarise the current literature surrounding genetics and PCOS, and secondly, to assess the methodological quality of current systematic reviews and identify limitations. Four databases were searched to identify candidate gene systematic reviews, and quality was assessed with the AMSTAR tool. Genome-wide association studies (GWAS) were identified by a semi structured literature search. Of the candidate gene systematic reviews, 17 were of high to moderate quality and four were of low quality. A total of 19 gene loci have been associated with risk of PCOS in GWAS, and 11 of these have been replicated across two different ancestries. Gene loci were located in the neuroendocrine, metabolic, and reproductive pathways. Overall, the gene loci with the most robust findings were THADA, FSHR, INS-VNTR, and DENND1A, that now require validation. This overview also identified limitations of the current literature and important methodological considerations for future genetic studies. Much work remains to identify causal variants and functional relevance of genes associated with PCOS.
Together We Stand, Divided We Fall: A Multidisciplinary Approach in Complicated Acute Pancreatitis
Authors: Paulino Jorge , Ramos Gonçalo , Veloso Gomes Filipe
Published: October 2019

Acute pancreatitis (AP) is an inflammatory condition with a mild course in most patients, but 20–30% evolve to single or multiple organ dysfunction and pancreatic/peripancreatic necrosis, with potentially infected collections. In the first weeks of disease, a systemic inflammatory syndrome (SIRS) dominates the clinical setting, and early management decisions in this precocious phase can change the course of the disease. Imaging is crucial in the diagnosis, and since the adoption of the revised Atlanta classification, four different types of pancreatic/peripancreatic collections have been defined. The management of the complicated forms of AP has been defined by several treatment guidelines, and the main indication for intervention is local infection, preferably in walled-off necrosis. Open surgery necrosectomy is associated with a very high rate of morbimortality, giving a place to different multidisciplinary methodologies, emphasizing drainage and necrosectomy techniques in a “step-up” approach starting from mini-invasive endoscopic drainage and moving, if needed, to progressively more invasive techniques, including interventional radiology and mini-invasive surgery. With the advent of several new technologies in the specialties involved, the complicated AP cases which need drainage and necrosectomy benefit from a new era of multidisciplinary cooperation, permitting higher efficacy with lower levels of morbimortality and reducing hospital stay and costs.
Tissue-Specific miRNAs Regulate the Development of Thoracic Aortic Aneurysm: The Emerging Role of KLF4 Network
Authors: Gasiulė Stasė , Stankevičius Vaidotas , Patamsytė Vaiva , Ražanskas Raimundas , Žukovas Giedrius , Kapustina Žana , Žaliaduonytė Diana , Benetis Rimantas , Lesauskaitė Vaiva , Vilkaitis Giedrius
Published: October 2019

MicroRNAs (miRNAs) are critical regulators of the functional pathways involved in the pathogenesis of cardiovascular diseases. Understanding of the disease-associated alterations in tissue and plasma will elucidate the roles of miRNA in modulation of gene expression throughout development of sporadic non-syndromic ascending thoracic aortic aneurysm (TAA). This will allow one to propose relevant biomarkers for diagnosis or new therapeutic targets for the treatment. The high-throughput sequencing revealed 20 and 17 TAA-specific miRNAs in tissue and plasma samples, respectively. qRT-PCR analysis in extended cohort revealed sex-related differences in miR-10a-5p, miR-126-3p, miR-155-5p and miR-148a-3p expression, which were the most significantly dysregulated in TAA tissues of male patients. Unexpectedly, the set of aneurysm-related miRNAs in TAA plasma did not resemble the tissue signature suggesting more complex organism response to the disease. Three of TAA-specific plasma miRNAs were found to be restored to normal level after aortic surgery, further signifying their relationship to the pathology. The panel of two plasma miRNAs, miR-122-3p, and miR-483-3p, could serve as a potential biomarker set (AUC = 0.84) for the ascending TAA. The miRNA-target enrichment analysis exposed TGF-β signaling pathway as sturdily affected by abnormally expressed miRNAs in the TAA tissue. Nearly half of TAA-specific miRNAs potentially regulate a key component in TGF-β signaling: TGF-β receptors, SMADs and KLF4. Indeed, using immunohistochemistry analysis we detected increased KLF4 expression in 27% of TAA cells compared to 10% of non-TAA cells. In addition, qRT-PCR demonstrated a significant upregulation of ALK1 mRNA expression in TAA tissues. Overall, these observations indicate that the alterations in miRNA expression are sex-dependent and play an essential role in TAA via TGF-β signaling.