Chitosan oligomer/silver nanoparticles/PCL composite electrospun membrane for severe burn wound healing
Authors:   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Que Anh Mai ,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Tang Tuan Ngan ,   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Thanh Tam Phan Thi ,   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Duong Thanh Tu ,   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Vu Thanh Binh ,   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Nguyen Van Khiem ,   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Doan Hoan Ngoc ,   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam, Nguyen Hiep Thi ,   Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Vietnam National University - Ho Chi Minh City, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam,   Vietnam National University - Ho Chi Minh City, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
Published: December 2023

In the management of burn wounds, antimicrobial and wound healing properties are crucial. In this study, we prepared a three-layer electrospun poly(ε-caprolactone) (PCL) membrane for burn wound dressing. The hydrophobic electrospun PCL layer, embedded with silver nanoparticles (SNPs) (PCL-Ag), was coated with another layer of electrospun plasma-treated PCL-Ag to enhance the wettability of the membrane. This enhancement was to facilitate the absorption of the hydrophilic chitosan oligomer (COS) - the third layer of the dressing. The resultant membranes were characterised and tested for different properties to demonstrate their applicability as wound dressing materials. The combination of COS and SNPs of the fabricated membrane supports the healing process and reduces burn severity due to the healing capacity of COS and the antibacterial activity of SNPs, without compromising mechanical strength. We conducted several in vitro and in vivo experiments to evaluate its applicability for burn wound healing. The PCL-Ag/COS sample demonstrated outstanding in vitro biocompatibility and excellent antibacterial activity against Staphylococcus aureus strains. We used the membrane to treat burns on rabbits in the laboratory over 30 days, and consistently observed positive outcomes. This research offers insights into the development of bioactive dressings for wound healing and opens up opportunities for practical applications of COS-incorporated materials.