bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Route of Mixed Catalysis for Production of Fatty Acid Methyl Esters from Potential Seed Oil Sources
Authors: Perveen Shazia , Hanif Muhammad Asif , Nadeem Razyia , Rashid Umer , Azeem Muhammad Waqar , Zubair Muhammad , Nisar Numrah , Alharthi Fahad A. , Moser Bryan R.
Published: July 2021

Depleting petroleum resources coupled with the environmental consequences of fossil fuel combustion have led to the search for renewable alternatives, such as biodiesel. In this study, sunflower (Helianthus annus), mustard (Brassica compestres) and pearl millet (Pennisetum americanum) seed oils were converted into biodiesel (fatty acid methyl esters) by acid-, base- and lipase-catalyzed transesterification, and the resultant fuel properties were determined. The methyl esters displayed superior iodine values (102–139), low densities, and a high cetane number (CN). The highest yield of biodiesel was obtained from mustard seed oil, which provided cloud (CP) and pour (PP) points of −3.5 and 5 °C, respectively, and a CN of 53. The sunflower seed oil methyl esters had a density of 0.81–0.86 kg/L at 16 °C, CP of 2 °C, PP of −8 °C, and a CN of 47. The pearl millet seed oil methyl esters yielded a density 0.87–0.89 kg/L, CP and PP of 4 °C and −5 °C, respectively, and a CN of 46. The major fatty acids identified in the sunflower, mustard, and pearl millet seed oils were linolenic (49.2%), oleic acid (82.2%), and linoleic acid (73.9%), respectively. The present study reports biodiesel with ideal values of CP and PP, to extend the use of biodiesel at the commercial level.
Bio-Derived Catalysts: A Current Trend of Catalysts Used in Biodiesel Production
Authors: Nguyen Hoang Chinh , Nguyen My-Linh , Su Chia-Hung , Ong Hwai Chyuan , Juan Horng-Yi , Wu Shao-Jung
Published: July 2021

Biodiesel is a promising alternative to fossil fuels and mainly produced from oils/fat through the (trans)esterification process. To enhance the reaction efficiency and simplify the production process, various catalysts have been introduced for biodiesel synthesis. Recently, the use of bio-derived catalysts has attracted more interest due to their high catalytic activity and ecofriendly properties. These catalysts include alkali catalysts, acid catalysts, and enzymes (biocatalysts), which are (bio)synthesized from various natural sources. This review summarizes the latest findings on these bio-derived catalysts, as well as their source and catalytic activity. The advantages and disadvantages of these catalysts are also discussed. These bio-based catalysts show a promising future and can be further used as a renewable catalyst for sustainable biodiesel production.
Inhibition of the Combinatorial Signaling of Transforming Growth Factor-Beta and NOTCH Promotes Myotube Formation of Human Pluripotent Stem Cell-Derived Skeletal Muscle Progenitor Cells
Authors: Choi In Young , Lim Ho Tae , Che Young Hyun , Lee Gabsang , Kim Yong Jun
Published: July 2021

Understanding the signaling pathways that regulate the final differentiation of human myoblasts is essential for successful cell transplantation and drug screening for the treatment of muscular dystrophy. In an effort to improve myotube formation from hiPSC-derived myoblasts, we validated a collection of 13 small molecules in a newly established in vitro screening platform for the assessment of myotube formation. The analysis of myotube formation as measured by the fusion index showed that the combinational inhibition of the TGFβ signaling with NOTCH signaling enhances the ability of multi-nucleated myotube production. Combinational treatment of inhibitors for TGFβ and NOTCH signaling pathways improved myotube formation in a dose-dependent manner. This effect was achieved by inhibiting the combinatorial mechanism of signaling. The combination treatment of small molecules effective in inducing multinucleated myotubes was validated in healthy human primary myoblasts. In addition, it was also applied to DMD patient iPSC-derived myoblasts to enhance the generation of multinucleated myotubes.
Altered Cytokine Response of Human Brain Endothelial Cells after Stimulation with Malaria Patient Plasma
Authors: Raacke Michaela , Kerr Amy , Dörpinghaus Michael , Brehmer Jana , Wu Yifan , Lorenzen Stephan , Fink Christine , Jacobs Thomas , Roeder Thomas , Sellau Julie , Bachmann Anna , Metwally Nahla Galal , Bruchhaus Iris
Published: July 2021

Infections with the deadliest malaria parasite, Plasmodium falciparum, are accompanied by a strong immunological response of the human host. To date, more than 30 cytokines have been detected in elevated levels in plasma of malaria patients compared to healthy controls. Endothelial cells (ECs) are a potential source of these cytokines, but so far it is not known if their cytokine secretion depends on the direct contact of the P. falciparum-infected erythrocytes (IEs) with ECs in terms of cytoadhesion. Culturing ECs with plasma from malaria patients (27 returning travellers) resulted in significantly increased secretion of IL-11, CXCL5, CXCL8, CXCL10, vascular endothelial growth factor (VEGF) and angiopoietin-like protein 4 (ANGPTL4) if compared to matching controls (22 healthy individuals). The accompanying transcriptome study of the ECs identified 43 genes that were significantly increased in expression (≥1.7 fold) after co-incubation with malaria patient plasma, including cxcl5 and angptl4. Further bioinformatic analyses revealed that biological processes such as cell migration, cell proliferation and tube development were particularly affected in these ECs. It can thus be postulated that not only the cytoadhesion of IEs, but also molecules in the plasma of malaria patients exerts an influence on ECs, and that not only the immunological response but also other processes, such as angiogenesis, are altered.
The Role of cAMP in Beta Cell Stimulus–Secretion and Intercellular Coupling
Authors: Stožer Andraž , Paradiž Leitgeb Eva , Pohorec Viljem , Dolenšek Jurij , Križančić Bombek Lidija , Gosak Marko , Skelin Klemen Maša
Published: July 2021

Pancreatic beta cells secrete insulin in response to stimulation with glucose and other nutrients, and impaired insulin secretion plays a central role in development of diabetes mellitus. Pharmacological management of diabetes includes various antidiabetic drugs, including incretins. The incretin hormones, glucagon-like peptide-1 and gastric inhibitory polypeptide, potentiate glucose-stimulated insulin secretion by binding to G protein-coupled receptors, resulting in stimulation of adenylate cyclase and production of the secondary messenger cAMP, which exerts its intracellular effects through activation of protein kinase A or the guanine nucleotide exchange protein 2A. The molecular mechanisms behind these two downstream signaling arms are still not fully elucidated and involve many steps in the stimulus–secretion coupling cascade, ranging from the proximal regulation of ion channel activity to the central Ca2+ signal and the most distal exocytosis. In addition to modifying intracellular coupling, the effect of cAMP on insulin secretion could also be at least partly explained by the impact on intercellular coupling. In this review, we systematically describe the possible roles of cAMP at these intra- and inter-cellular signaling nodes, keeping in mind the relevance for the whole organism and translation to humans.
Patient and Family-Centered Care for Pediatric Intraluminal Pulmonary Vein Stenosis: Case of a 3 Year Old Patient with Focus on Nurse Practitioner Role
Authors: Ireland Christina M. , Callahan Ryan , Jenkins Kathy J.
Published: July 2021

A nurse practitioner’s experience in managing children with intraluminal pulmonary vein stenosis. A case study of a 3-year-old patient with multi–vessel intraluminal pulmonary vein stenosis.
Radiation-Induced Lung Injury—Current Perspectives and Management
Authors: Rahi Mandeep Singh , Parekh Jay , Pednekar Prachi , Parmar Gaurav , Abraham Soniya , Nasir Samar , Subramaniyam Rajamurugan , Jeyashanmugaraja Gini Priyadharshini , Gunasekaran Kulothungan
Published: July 2021

Radiotherapy plays an important role in the treatment of localized primary malignancies involving the chest wall or intrathoracic malignancies. Secondary effects of radiotherapy on the lung result in radiation-induced lung disease. The phases of lung injury from radiation range from acute pneumonitis to chronic pulmonary fibrosis. Radiation pneumonitis is a clinical diagnosis based on the history of radiation, imaging findings, and the presence of classic symptoms after exclusion of infection, pulmonary embolism, heart failure, drug-induced pneumonitis, and progression of the primary tumor. Computed tomography (CT) is the preferred imaging modality as it provides a better picture of parenchymal changes. Lung biopsy is rarely required for the diagnosis. Treatment is necessary only for symptomatic patients. Mild symptoms can be treated with inhaled steroids while subacute to moderate symptoms with impaired lung function require oral corticosteroids. Patients who do not tolerate or are refractory to steroids can be considered for treatment with immunosuppressive agents such as azathioprine and cyclosporine. Improvements in radiation technique, as well as early diagnosis and appropriate treatment with high-dose steroids, will lead to lower rates of pneumonitis and an overall good prognosis.
Recent Developments in Coatings for Orthopedic Metallic Implants
Authors: Hussain Muzamil , Askari Rizvi Syed Hasan , Abbas Naseem , Sajjad Uzair , Shad Muhammad Rizwan , Badshah Mohsin Ali , Malik Asif Iqbal
Published: July 2021

Titanium, stainless steel, and CoCrMo alloys are the most widely used biomaterials for orthopedic applications. The most common causes of orthopedic implant failure after implantation are infections, inflammatory response, least corrosion resistance, mismatch in elastic modulus, stress shielding, and excessive wear. To address the problems associated with implant materials, different modifications related to design, materials, and surface have been developed. Among the different methods, coating is an effective method to improve the performance of implant materials. In this article, a comprehensive review of recent studies has been carried out to summarize the impact of coating materials on metallic implants. The antibacterial characteristics, biodegradability, biocompatibility, corrosion behavior, and mechanical properties for performance evaluation are briefly summarized. Different effective coating techniques, coating materials, and additives have been summarized. The results are useful to produce the coating with optimized properties.
The Effects on Thermal Efficiency of Yttria-Stabilized Zirconia and Lanthanum Zirconate-Based Thermal Barrier Coatings on Aluminum Heating Block for 3D Printer
Authors: Demir Hasan
Published: July 2021

Fused filament fabrication is an important additive manufacturing method, for which 3D printers are the most commonly used printing tools. In this method, there are many factors that affect the printing quality, chief among which is temperature. The fusion temperature of the material is created by an aluminum heating block in the extruder. Stability and a constant temperature for the aluminum heating block are inevitable requirements for print quality. This study aims to use the thermal barrier coating method to increase the thermal efficiency and stability of the aluminum heating block by reducing heat loss. Furthermore, it aims to perform steady-state thermal analysis using finite element analysis software. The analyses are carried out in stagnant air environment and at the printing temperature of acrylonitrile butadiene styrene material. In order to examine the effects of different coating materials, blocks coated with two different coating materials, as well as uncoated blocks, were used in the analyses. The coating made with yttria-stabilized zirconia and pyrochlore-type lanthanum zirconate materials, together with the NiCRAl bond layer, prevent temperature fluctuation by preventing heat loss. The effects of the coating method on average heat flux density, temperature distribution of blocks, and temperature distribution of the filament tube hole were investigated. Additionally, changes in flow velocity were determined by examining the effects of the thermal barrier coating method on temperature distribution. The average heat flux density in the coated blocks decreased by 10.258%. Throughout the investigation, the temperature distributions in the coated blocks became homogeneous. It was also observed that both coating materials produce the same effect. This article performs a steady-state thermal analysis of a conventional model and thermal-barrier-coated models to increase print quality by reducing heat loss from the aluminum heating block.
Investigation of the Long-Term Antibacterial Properties of Titanium by Two-Step Micro-Arc Oxidation Treatment
Authors: Tsutsumi Harumi , Tsutsumi Yusuke , Shimabukuro Masaya , Manaka Tomoyo , Chen Peng , Ashida Maki , Ishikawa Kunio , Katayama Hideki , Hanawa Takao
Published: July 2021

Recently, biofilm formation caused by bacterial adhesion and colonization has been recognized as the major cause of failure in orthopedic and dental implant surgeries. In this study, a customized micro-arc oxidation (MAO) treatment technique was developed to obtain desirable antibacterial properties on Ti surfaces. The two-step MAO treatment was applied in the fabrication of specimens with Ag and with/without Zn in their surface oxide layer. In order to simulate practical usage, surface analyses and immersion tests were performed to evaluate the incorporation of Ag and Zn into the resulting oxide layer and ion release behavior, respectively. Additionally, the antibacterial properties of the specimens after long-term immersion in physiological saline were evaluated using Gram-negative facultative anaerobic bacteria. The MAO-treated specimens containing Ag and Zn exhibited excellent antibacterial properties against Escherichia coli, which were sustained even after 6 months of immersion in physiological saline to simulate practical usage. Moreover, the Ag ions released from the surface oxide indicate the antibacterial properties of the specimen in the early stage, while the release of the corrosion products of Zn demonstrates its antibacterial properties in the later stage.
Anaerobic Mineralization of Recirculating Aquaculture Drum Screen Effluent for Use as a Naturally-Derived Nutrient Solution in Hydroponic Cropping Systems
Authors: Tetreault Joseph , Fogle Rachel , Guerdat Todd
Published: July 2021

Operation and effluent treatment costs are limiting factors for the success of recirculating aquaculture systems (RAS) in meeting seafood demand in the United States. Adopting a capture-and-reuse waste management model similar to terrestrial agriculture farmers would allow RAS farmers to monetize effluent and offset production costs. The moisture content and nutrient profile of RAS effluent makes it a potential option for use as a hydroponic fertilizer. Treatment of RAS waste is needed to mineralize particulate-bound nutrients before becoming a viable hydroponic nutrient solution. Anaerobic treatment (AT), a method used by municipal and agricultural waste treatment facilities to reduce total solids, has been shown to successfully mineralize particulate-bound nutrients from RAS effluent. Continuously mixed anaerobic batch bioreactors were used to evaluate the degree to which AT may mineralize particulate-bound nutrients in solid RAS waste. Concentrations of twelve different macro- and micro-nutrients were analyzed in the waste before and after treatment. Effluent samples were analyzed to determine the fraction of each nutrient in the solid and aqueous forms. This study showed that AT is an effective method to mineralize particulate-bound nutrients in RAS effluent and the mineralization rate data may be used to design a pilot-scaled flow-through RAS effluent treatment system.
Sulforaphane, L-Menthol, and Dexpanthenol as a Novel Active Cosmetic Ingredient Composition for Relieving Hair Loss Symptoms
Authors: Park Youngkum , Choi Kanghyun , Kim Hyoseon , Lee Jihye , Park Gyeryeol , Kim Junehyun
Published: July 2021

Sulforaphane increases the expression of the dihydrotestosterone (DHT)-degrading enzyme, 3α-hydroxysteroid dehydrogenase (3α-HSD) in the liver, which accelerates DHT degradation, thereby inhibiting hair loss in the animal model. In this study, we elucidated its underlying mechanism and demonstrated that sulforaphane has hair loss inhibitory functions in RAW264.7 macrophage cells and Hepa1c1c7 cells at the cellular and gene levels. The gene expression level of an isoform of 3α-HSD, Akr1c2, increased in a dose-dependent manner when these cells were treated with sulforaphane, but there were no significant differences at the gene levels of Akr1c2 and Dhrs9 for the negative control mixture of biotin, dexpanthenol, and L-menthol. These studies indicated that sulforaphane is involved in regulating the gene expression of Akr1c2. To further determine whether this hair product has effects on alleviating hair loss symptoms, clinical trials were also conducted for 18 weeks. We performed a visual evaluation of the parietal and frontal lines of 23 patients before and after using the product, and then calculated the total number of hairs. This clinical study showed that the parietal lines and bangs visually improved and the number of hairs increased by 6.71% from before using the test product to 18 weeks after using the test product. Taken together, these cellular and clinical studies strongly suggest that sulforaphane may be an active ingredient that significantly alleviates hair loss symptoms.
Diversity and Adaptation of Currently Grown Wheat Landraces and Modern Germplasm in Afghanistan, Iran, and Turkey
Authors: Morgounov Alexey , Özdemir Fatih , Keser Mesut , Akin Beyhan , Dababat Abdelfattah A. , Dreisigacker Susanne , Golkari Saber , Koc Emrah , Küçükçongar Murat , Muminjanov Hafiz , Nehe Ajit , Rasheed Awais , Roostaei Mozaffar , Sehgal Deepmala , Sharma Rajiv
Published: July 2021

Collection of wheat landraces (WLR) was conducted in Afghanistan, Iran, and Turkey in 2010–2014. A representative subset of this collection was used in the current study and included 45 bread wheat landraces from Turkey, 19 from Iran, and 20 from Afghanistan. This material was supplemented by 73 modern cultivars and breeding lines adapted to semiarid conditions and irrigated conditions. Overall, 157 genotypes were tested in Turkey in 2018 and 2019 and in Afghanistan and Iran in 2019 under rainfed conditions to compare performance of WLR and modern material. The germplasm was genotyped using a high density Illumina Infinium 25K wheat SNP array and KASP markers for agronomic traits. The average grain yield ranged between 2.2 and 4.0 t/ha depending on the site and year. Three groups of landraces demonstrated similar average grain yield, though Afghanistan material was slightly higher yielding not only in Afghanistan but also in Turkey. Modern material outyielded the landraces in two environments out of four. The highest yielding landraces were competitive with the best modern germplasm. Frequency of gene Sus2-2B affecting 1000 kernel weight was 64% in WLR and only 3% in modern material. Presence of positive allele of Sus2-2B increased 1000 kernel weight by nearly 4%. Breeding strategy to improved landraces and modern cultivars is discussed.
Refinement of RNA Structures Using Amber Force Fields
Authors: Gray Jonathon G. , Case David A.
Published: July 2021

Atomic models for nucleic acids derived from X-ray diffraction data at low resolution provide much useful information, but the observed scattering intensities can be fit with models that can differ in structural detail. Tradtional geometric restraints favor models that have bond length and angle terms derived from small molecule crystal structures. Here we explore replacing these restraints with energy gradients derived from force fields, including recently developed integral equation models to account for the effects of water molecules and ions that are not part of the explicit model. We compare conventional and force-field based refinements for 22 RNA crystals, ranging in resolution from 1.1 to 3.6 Å. As expected, it can be important to account for solvent screening of charge–charge interactions, especially in the crowded environment of a nucleic acid crystal. The newly refined models can show improvements in torsion angles and hydrogen-bonding interactions, and can significantly reduce unfavorable atomic clashes, while maintaining or improving agreement with observed scattering intensities.
Overcoming of Microenvironment Protection on Primary Chronic Lymphocytic Leukemia Cells after Treatment with BTK and MDM2 Pharmacological Inhibitors
Authors: Rimondi Erika , Melloni Elisabetta , Romani Arianna , Tisato Veronica , Casciano Fabio , Rigolin Gian Matteo , Milani Daniela , Celeghini Claudio , Zauli Giorgio , Secchiero Paola , Voltan Rebecca
Published: July 2021

In B-chronic lymphocytic leukemia (B-CLL), the interaction between leukemic cells and the microenvironment promotes tumor cell survival. The Bruton’s tyrosine kinase (BTK) inhibitor ibrutinib is one of the first-in-class molecules for the treatment of B-CLL patients; however, the emerging mechanisms of resistance to ibrutinib call for new therapeutic strategies. The purpose of the current study was to investigate the ability of ibrutinib plus the MDM2-inhibitor nutlin-3 to counteract the tumor microenvironment protective effect. We observed that primary B-CLL cells cultivated in microenvironment mimicking conditions were protected from apoptosis by the up-regulation of c-MYC and of p53. In the same setting, combined treatments with ibrutinib plus nutlin-3 led to significantly higher levels of apoptosis compared to the single treatments, counteracting the c-MYC up-regulation. Moreover, the combination induced high p53 levels and a significant dissipation of the mitochondrial membrane potential, together with BAX cleavage in the more active p18 form and phospho-BAD down-regulation, that are key components of the mitochondrial apoptotic pathway, enhancing the apoptosis level. Our findings propose a new therapeutic strategy to overcome the tumor microenvironment protection involved in B-CLL resistance to drugs, with possible clinical implications also for other hematologic and solid tumors for which ibrutinib is considered a therapeutic option.
Computational Study of the Propeller Position Effects in Wing-Mounted, Distributed Electric Propulsion with Boundary Layer Ingestion in a 25 kg Remotely Piloted Aircraft
Authors: Serrano José Ramón , Tiseira Andrés Omar , García-Cuevas Luis Miguel , Varela Pau
Published: July 2021

Distributed electric propulsion and boundary layer ingestion are two attractive technologies to reduce the power consumption of fixed wing aircraft. Through careful distribution of the propulsive system elements, higher aerodynamic and propulsive efficiency can be achieved, as well as a lower risk of total loss of aircraft due to foreign object damage. When used on the wing, further reductions of the bending moment on the wing root can even lead to reductions of its structural weight, thus mitigating the expected increase of operating empty weight due to the extra components needed. While coupling these technologies in fixed-wing aircraft is being actively studied in the big aircraft segment, it is also an interesting approach for increasing the efficiency even for aircraft with maximum take-off masses as low as 25 kg, such as the A3 open subcategory for civil drones from EASA. This paper studies the effect of changing the propellers’ position in the aerodynamic performance parameters of a distributed electric propulsion with boundary layer ingestion system in a 25 kg fixed-wing aircraft, as well as in the performance of the propellers. The computational results show the trade-offs between the aerodynamic efficiency and the propeller efficiency when the vertical position is varied.
Development of Methodology and Assessment of Ecological Safety of the EAEU and CIS Regions in the Context of Sustainable Development
Authors: Demidova Svetlana , Balog Michael , Chircova Tatiana , Kulachinskaya Anastasia , Zueva Svetlana , Akhmetova Irina , Ilyashenko Svetlana
Published: July 2021

The trends of sustainable development and green agenda transform the production processes, leading industries, and regional markets, and reveal objective contradictions in ensuring the ecological safety of certain territories. This study aims to develop a methodology and assess ecological safety at aggregated system levels, taking into account natural and socioeconomic factors as well as the factor of human capital development with specification of the place of ecological safety in the concept of sustainable development. The objects of empirical research are the EAEU and CIS countries for the period 2010–2019, as well as 85 Russian regions. The scientific research toolkit is based on the methods of structural-logical, economical-statistical, and comparative analyses, and expert judgments. The concepts of sustainable development and human capital and the theory of the national school of ecological safety are analyzed using the systematic approach. The mixed methodological approach showed the interconnection of four approaches of assessing ecological safety (technogenic, environmental, institutional, resource-based). The authors developed a method for assessing ecological safety taking into account the contribution of human capital. The assessment results are typologized and grouped according to the dynamic trend. For the majority of the considered countries, changes in the level of ecological safety correlate with changes in socioeconomic indicators. The same trend is observed when considering the Russian regional formations. The contribution of human capital was recorded to a lesser extent. For several regions, the increased number of objects polluting the environment is accompanied by a decreased volume of polluting emissions into the atmosphere.
Elucidation and Regulation of Polyphenols in the Smoking Process of Shanxi Aged Vinegar
Authors: Xie Sankuan , Song Jia , Fan Bingqian , Li Xuan , Li Yingqi , Mou Fangming , Zheng Yu , Wang Min
Published: July 2021

Polyphenols (PPs) are the main contributors to the health functions of Shanxi aged vinegar (SAV) and are mainly produced during the smoking process. This study aimed to explore the feasibility of regulating the accumulation of total water-soluble PPs (TWSP) by changing environmental factors based on the distribution of PPs. A total of eleven PPs, such as vanillin, vanillic acid, and (e)-ferulic acid, were detected during the smoking process. During the smoking process, the content of TWSP gradually increased and was accompanied by changes in environmental factors. Spearman correlation analysis and verification experiments showed that temperature, amino acids, and reducing sugars, as the main influencing factors, promoted the accumulation of TWSP. The in situ regulation strategy of changing environmental factors significantly increased the accumulation of TWSP by 12.24%.
Analysis of Food Production and Consumption Based on the Emergy Method in Kazakhstan
Authors: Jia Mengmeng , Zhen Lin
Published: July 2021

The imbalance in the supply and demand for resources is a thorny issue that many countries have to face. Food is a basic resource throughout the world. Understanding the exact situation of food production and consumption is an important foundation for sustainable development. This paper aims to explore the quantity and pattern changes in food production and consumption in Kazakhstan. This can reflect the level of residents’ standard of living and the situation of natural resource use. This paper focuses on the quantitative relationship between animal-based food and plant-based food and the tendency towards changes in urban and rural residents’ food production and consumption in Kazakhstan based on the emergy analysis method. The data of food production and consumption were mainly obtained from the official statistics of the Food and Agriculture Organization (FAO), the World Bank, Statistical Commission of the Ministry of National Economy of Kazakhstan, and literature. The research results showed that, over time, Kazakhstan residents’ food consumption patterns have become more varied, and the proportion of meat consumption to total food consumption has increased. Although the rural per capita food consumption is significantly different from that of urban residents, this gap decreased in 2019. In Kazakhstan, the consumption of several types of food still relies on imports. Food production and consumption are affected by economic, social, and ecological factors. The results of this research can provide scientific support for reasonable and sustainable production and consumption strategies in Kazakhstan.
The Feasibility of Using Pulsed-Vacuum in Stimulating Calcium-Alginate Hydrogel Balls
Authors: Jinnoros Janjira , Innawong Bhundit , Udomkun Patchimaporn , Parakulsuksatid Pramuk , Silva Juan L.
Published: July 2021

The effect of the pulsed-vacuum stimulation (PVS) on the external gelation process of calcium-alginate (Ca-Alg) hydrogel balls was studied. The process was conducted at four different working pressures (8, 35, 61, and 101 kPa) for three pulsed-vacuum cycles (one cycle consisted of three repetitions of 10 min of depressurization and 10 min of vacuum liberation). The diffusion coefficients (D) of calcium cations (Ca2+) gradually reduced over time and were significantly pronounced (p < 0.05) at the first three hours of the external gelation process. The rate of weight reduction (WR) and rate of volume shrinkage (Sv) varied directly according to the D value of Ca2+. A significant linear relationship between WR and Sv was observed for all working pressures (R2 > 0.91). An application of a pulsed vacuum at 8 kPa led to the highest weight reduction and shrinkage of Ca-Alg hydrogel samples compared to other working pressures, while 61 kPa seemed to be the best condition. Although all textural characteristics (hardness, breaking deformation, Young’s modulus, and rupture strength) did not directly variate by the level of working pressures, they were likely correlated with the levels of WR and Sv. Scanning electron micrographs (SEM) supported that the working pressure affected the characteristics of Ca-Alg hydrogel structure. Samples stimulated at a working pressure of 8 kPa showed higher deformation with heterogenous structure, large cavities, and looser layer when compared with those at 61 kPa. These results indicate the PVS is a promising technology that can be effectively applied in the external gelation process of Ca-Alg gel.
The Phenotypic Spectrum of 15q13.3 Region Duplications: Report of 5 Patients
Authors: Budisteanu Magdalena , Papuc Sorina Mihaela , Streata Ioana , Cucu Mihai , Pirvu Andrei , Serban-Sosoi Simona , Erbescu Alina , Andrei Emanuela , Iliescu Catrinel , Ioana Doina , Severin Emilia , Ioana Mihai , Arghir Aurora
Published: July 2021

Chromosome 15q13.3 microduplications are associated with a wide spectrum of clinical presentations ranging from normal to different neuropsychiatric conditions, such as developmental delay (DD), intellectual disability (ID), epilepsy, hypotonia, autism spectrum disorders (ASD), attention-deficit hyperactivity disorder, and schizophrenia. The smallest region of overlap for 15q13.3 duplications encompasses the Cholinergic Receptor Nicotinic Alpha 7 Subunit (CHRNA7) gene, a strong candidate for the behavioral abnormalities. We report on a series of five patients with 15q13.3 duplications detected by chromosomal microarray. The size of the duplications ranged from 378 to 537 kb, and involved the CHRNA7 gene in all patients. The most common clinical features, present in all patients, were speech delay, autistic behavior, and muscle hypotonia; DD/ID was present in three patients. One patient presented epileptic seizures; EEG anomalies were observed in three patients. No consistent dysmorphic features were noted. Neuroimaging studies revealed anomalies in two patients: Dandy–Walker malformation and a right temporal cyst. 15q13.3 duplications are associated with various neuropsychiatric features, including speech delay, hypotonia, ASD, and ID, also present in our patient group. Our study brings detailed clinical and molecular data from five ASD patients with 15q13.3 microduplications involving the CHRNA7 gene, contributing to the existing knowledge about the association of 15q13.3 duplications with neuropsychiatric phenotypes.
CRISPR/Cas9-Mediated Specific Integration of Fat-1 and IGF-1 at the pRosa26 Locus
Authors: You Wenni , Li Mengjing , Qi Yilin , Wang Yanbing , Chen Yiwu , Liu Ying , Li Li , Ouyang Hongsheng , Pang Daxin
Published: July 2021

Many researchers have focused on knock-in pigs for site-specific integration, but little attention has been given to genetically modified pigs with the targeted integration of multiple recombinant genes. To establish a multigene targeted knock-in editing system, we used the internal ribosome entry site (IRES) and self-cleaving 2A peptide technology to construct a plasmid coexpressing the fatty acid desaturase (Fat-1) and porcine insulin-like growth factor-1 (IGF-1) genes at equal levels. In this study, pigs were genetically modified with multiple genes that were precisely inserted into the pRosa26 locus by using the clustered regularly spaced short palindrome repeat sequence (CRISPR)/CRISPR-related 9 (Cas9) system and somatic cell nuclear transfer technology (SCNT) in combination. Single copies of the Fat-1 and IGF-1 genes were expressed satisfactorily in various tissues of F0-generation pigs. Importantly, gas chromatography analysis revealed a significantly increased n-3 polyunsaturated fatty acid (PUFA) level in these genetically modified pigs, which led to a significant decrease of the n-6 PUFA/n-3 PUFA ratio from 6.982 to 3.122 (*** p < 0.001). In conclusion, the establishment of an editing system for targeted double-gene knock-in in this study provides a reference for the precise integration of multiple foreign genes and lays a foundation for the development of new transgenic pig breeds with multiple excellent phenotypes.
Total Knee Arthroplasty in Octogenarians: Should We Still Be so Restrictive?
Authors: Trigueros-Larrea Jose Maria , Gonzalez-Bedia Maria Antonia , Lomo-Garrote Jose Maria , Martin-de la Cal Oscar , Martin-Ferrero Miguel Angel
Published: July 2021

Demand for total knee arthroplasty (TKA) in octogenarians will increase in subsequent years as society ages. We conducted a retrospective observational study in octogenarians operated on with TKA between 2015 and 2019, comparing preoperative and postoperative Knee Society Score (KSS), Knee Society Function Score (KSFS), extension and flexion balance, and radiologic alignment using a paired Student t-test. A chi-squared test was used to correlate mortality with Charlson comorbidities index score and with ASA scale. Kaplan–Meier analysis was performed to calculate patient survival. In this period 36 patients ≥80 years underwent TKA, with a mean age of 81.6 years. Of these, 24 patients (66.7%) were classified as ASA II and 12 (33.3%) as ASA III. Sixteen patients (44.4%) were Charlson 0, 14 (38.9%) Charlson 1, two (5.6%) Charlson 2, and four (11.1%) Charlson 3. KSS, KSFS, flexion and extension range, and radiologic alignment were statistically significant (p < 0.001) when comparing preoperatory and post-operatory data. No correlation (p > 0.05) was found between mortality and ASA or Charlson score. Seven patients (19.4%) suffered a medical complication and two patients experienced surgical complications. Four patient died (11.1%) during follow-up. The mean patient survival was 67.4 months. Patients ≥80 years achieve clinical improvement after TKA. Comorbidities, not age, are the burden for surgery in older patients.
“Underground Built Heritage”: A Theoretical Approach for the Definition of an International Class
Authors: Varriale Roberta
Published: July 2021

Although nowadays sustainable reuse of underground cultural heritage has become a global trend, as yet Underground Built Heritage (UBH) is not regarded as a distinctive class eligible for protection. After a critical overview of previous attempts at defining underground heritage by associations such as UIS, SSI and UNESCO, this article updates the definition of the new-born class of UBH on the basis of three main criteria: position (by introducing the concept of Geographical Zero Level), manmade character, and cultural relevance, both material and immaterial. Building on the outputs of several projects devoted to this topic and the results of academic expertise in this field, the author proposes a new dedicated methodological approach consisting of a chart for the classification of artefacts as historical UBH and a strategy for their reuse based on a four-level scale: Re-inventing, Re-introducing, Re-interpreting and Re-building.
Reply to Ialongo et al. Vitamin D, SARS-CoV-2 and Causal Associations in Transversal Studies: The Time-Series Analysis to Reveal Potential Confounders. Comment on “Gaudio et al. Vitamin D Levels Are Reduced at the Time of Hospital Admission in Sicilian SARS-CoV-2-Positive Patients. Int. J. Environ. Res. Public Health 2021, 18, 3491”
Authors: Gaudio Agostino , Murabito Andrea , Agodi Antonella , Montineri Arturo , Castellino Pietro ,   D.O.CoV Research
Published: July 2021

We read the comment by Ialongo et al. [...]
Hospital Admissions Due to Ischemic Heart Diseases and Prescriptions of Cardiovascular Diseases Medications in England and Wales in the Past Two Decades
Authors: Hemmo Sara Ibrahim , Naser Abdallah Y. , Alwafi Hassan , Mansour Munthir M. , Alanazi Abeer F. R. , Jalal Zahraa , Alsairafi Zahra Khalil , Paudyal Vibhu , Alomari Esra’a , Al-Momani Hamzeh , Salawati Emad M. , Samannodi Mohammed , Dairi Mohammad S. , Al Bawab Abdel Qader , Mustafa Ali Moaath K. , Alkharabsheh Saqer
Published: July 2021

Objectives: The aim of this study was to explore the trend of ischemic heart disease (IHD) admission and the prescriptions of IHD medications in England and Wales. Methods: A secular trends study was conducted during the period of 1999 to 2019. We extracted hospital admission data for patients from all age groups from the Hospital Episode Statistics database in England and the Patient Episode Database for Wales. Prescriptions of IHD medications were extracted from the Prescription Cost Analysis database from 2004 to 2019. The chi-squared test was used to assess the difference between the admission rates and the difference between IHD medication prescription rates. The trends in IHD-related hospital admission and IHD-related medication prescription were assessed using a Poisson model. The correlation between hospital admissions for IHD and its IHD medication-related prescriptions was assessed using the Pearson correlation coefficient. Results: Our study detected a significant increase in the rate of cardiovascular disease (CVD) medication prescriptions in England and Wales, representing a rise in the CVD medications prescription rate of 41.8% (from 539,334.95 (95% CI = 539,286.30–539,383.59) in 2004 to 764,584.55 (95% CI = 764,545.55–764,623.56) in 2019 prescriptions per 100,000 persons), with a mean increase of 2.8% per year during the past 15 years. This increase was connected with a reduction in the IHD hospital admission rate by 15.4% (from 838.50 (95% CI = 836.05–840.94) in 2004 to 709.78 (95% CI = 707.65–711.92) in 2019 per 100,000 persons, trend test, p < 0.01), with a mean decrease of 1.02% per year during the past 15 years and by 5% (from 747.43 (95% CI = 745.09–749.77) in 1999 to 709.78 (95% CI = 707.65–711.92) in 2019 per 100,000 persons, trend test, p < 0.01) with a mean decrease of 0.25% per year during the past two decades in England and Wales. Conclusion: The rate of hospitalisation due to IHD has decreased in England and Wales during the past two decades. Hospitalisation due to IHD was strongly and negatively correlated with the increase in the rates of dispensing of IHD-related medications. Other factors contributing to this decline could be the increase in controlling IHD risk factors during the past few years. Future studies exploring other risk factors that are associated with IHD hospitalisation are warranted.
Development and Validation of Vulnerable and Enabling Indices for HIV Viral Suppression among People with HIV Enrolled in the Ryan White Program
Authors: Gebrezgi Merhawi T. , Trepka Mary Jo , Gbadamosi Semiu O. , Fennie Kristopher P. , Ramirez-Ortiz Daisy , Li Tan , Fernandez Sofia B. , Brock Petra , Ladner Robert A. , Sheehan Diana M.
Published: July 2021

Background: Numerous factors impact HIV care, often requiring consideration of indices to prevent collinearity when using statistical modeling. Using the Behavioral Model for Vulnerable Populations, we developed vulnerable and enabling indices for people living with HIV (PLWH). Methods: We used Ryan White Program (RWP) data and principal component analysis to develop general and gender- and racial/ethnic-specific indices. We assessed internal reliability (Cronbach’s alpha), convergent validity (correlation coefficient), and predictive utility (logistic regression) with non-viral suppression. Results: Three general factors accounting for 79.2% of indicators’ variability surfaced: mental health, drug use, and socioeconomic status (Cronbach’s alpha 0.68). Among the overall RWP population, indices showed convergent validity and predictive utility. Using gender- or racial/ethnic-specific indices did not improve psychometric performance. Discussion: General mental health, drug use, and socioeconomic indices using administrative data showed acceptable reliability, validity, and utility for non-viral suppression in an overall PLWH population and in gender- and racial/ethnic-stratified populations. These general indices may be used with similar validity and utility across gender and racial/ethnic diverse populations.
Effects of a Floor Hockey Intervention on Motor Proficiency, Physical Fitness, and Adaptive Development in Youths with Mild Intellectual Disabilities
Authors: Hsu Po-Jen , Yeh Hung-Ling , Tsai Chia-Liang , Chu Chia-Hua , Chen Fu-Chen , Pan Chien-Yu
Published: July 2021

This study examined the effects of a 12-week floor hockey training program on the motor proficiency, physical fitness, and adaptive development of youths with mild intellectual disabilities (IDs). A total of 54 youths with IDs were divided into two groups: a floor hockey exercise group (EG; n = 27; age, 16.59 ± 0.56 years) and a control group (CG; n = 27; age, 16.65 ± 0.63 years). The participants in the EG attended sessions of a floor hockey training program 3 times per week over a 12-week period. The CG group maintained their standard activities of daily living. The participants’ scores on the Bruininks–Oseretsky Test of Motor Proficiency, Second Edition, Brockport Physical Fitness Test, and traditional Chinese version of the teacher form of the Adaptive Behavior Assessment System, Second Edition, were obtained before and after the intervention. The results of the study indicate that the 12-week floor hockey training program significantly increased the participants’ scores for most indicators of motor proficiency (p < 0.01), physical fitness (p < 0.01), and adaptive development (p < 0.01). The findings provide evidence that physical activity interventions focusing on floor hockey training are a viable therapeutic option for treating youths with IDs.
DEM- and GIS-Based Analysis of Soil Erosion Depth Using Machine Learning
Authors: Nguyen Kieu , Chen Walter
Published: July 2021

Soil erosion is a form of land degradation. It is the process of moving surface soil with the action of external forces such as wind or water. Tillage also causes soil erosion. As outlined by the United Nations Sustainable Development Goal (UN SDG) #15, it is a global challenge to “combat desertification, and halt and reverse land degradation and halt biodiversity loss.” In order to advance this goal, we studied and modeled the soil erosion depth of a typical watershed in Taiwan using 26 morphometric factors derived from a digital elevation model (DEM) and 10 environmental factors. Feature selection was performed using the Boruta algorithm to determine 15 factors with confirmed importance and one tentative factor. Then, machine learning models, including the random forest (RF) and gradient boosting machine (GBM), were used to create prediction models validated by erosion pin measurements. The results show that GBM, coupled with 15 important factors (confirmed), achieved the best result in the context of root mean square error (RMSE) and Nash–Sutcliffe efficiency (NSE). Finally, we present the maps of soil erosion depth using the two machine learning models. The maps are useful for conservation planning and mitigating future soil erosion.
Metal–Curcumin Complexes in Therapeutics: An Approach to Enhance Pharmacological Effects of Curcumin
Authors: Prasad Sahdeo , DuBourdieu Dan , Srivastava Ajay , Kumar Prafulla , Lall Rajiv
Published: July 2021

Curcumin, an active component of the rhizome turmeric, has gained much attention as a plant-based compound with pleiotropic pharmacological properties. It possesses anti-inflammatory, antioxidant, hypoglycemic, antimicrobial, neuroprotective, and immunomodulatory activities. However, the health-promoting utility of curcumin is constrained due to its hydrophobic nature, water insolubility, poor bioavailability, rapid metabolism, and systemic elimination. Therefore, an innovative stride was taken, and complexes of metals with curcumin have been synthesized. Curcumin usually reacts with metals through the β-diketone moiety to generate metal–curcumin complexes. It is well established that curcumin strongly chelates several metal ions, including boron, cobalt, copper, gallium, gadolinium, gold, lanthanum, manganese, nickel, iron, palladium, platinum, ruthenium, silver, vanadium, and zinc. In this review, the pharmacological, chemopreventive, and therapeutic activities of metal–curcumin complexes are discussed. Metal–curcumin complexes increase the solubility, cellular uptake, and bioavailability and improve the antioxidant, anti-inflammatory, antimicrobial, and antiviral effects of curcumin. Metal–curcumin complexes have also demonstrated efficacy against various chronic diseases, including cancer, arthritis, osteoporosis, and neurological disorders such as Alzheimer’s disease. These biological activities of metal–curcumin complexes were associated with the modulation of inflammatory mediators, transcription factors, protein kinases, antiapoptotic proteins, lipid peroxidation, and antioxidant enzymes. In addition, metal–curcumin complexes have shown usefulness in biological imaging and radioimaging. The future use of metal–curcumin complexes may represent a new approach in the prevention and treatment of chronic diseases.
The Vitreous Ecosystem in Diabetic Retinopathy: Insight into the Patho-Mechanisms of Disease
Authors: Iyer Siva S.R. , Lagrew Mollie K. , Tillit Stephanie M. , Roohipourmoallai Ramak , Korntner Samuel
Published: July 2021

Diabetic retinopathy is one of the leading causes of blindness in the world with the incidence of disease ever-increasing worldwide. The vitreous humor represents an extensive and complex interactive arena for cytokines in the diabetic eye. In recent decades, there has been significant progress in understanding this environment and its implications in disease pathophysiology. In this review, we investigate the vitreous ecosystem in diabetic retinopathy at the molecular level. Areas of concentration include: the current level of knowledge of growth factors, cytokine and chemokine mediators, and lipid-derived metabolites in the vitreous. We discuss the molecular patho-mechanisms of diabetic retinopathy based upon current vitreous research.