bluuebunny/crossref_metadata_2025 huggingface dataset:
ctDNA for the Evaluation and Management of EGFR-Mutant Non-Small Cell Lung Cancer
Authors: Desai Aakash , Vázquez Tadana A. , Arce Keishla M. , Corassa Marcelo , Mack Philip C. , Gray Jhanelle E. , Pellini Bruna
Published: February 2024

Circulating tumor DNA (ctDNA) offers a new paradigm in optimizing treatment strategies for epidermal growth factor receptor (EGFR) mutant non-small cell lung cancer (NSCLC). Its potential spans early-stage disease, influencing adjuvant therapy, to advanced disease, where it aids in identifying genomic markers and resistance mechanisms. This review explores the evolving landscape of utilizing liquid biopsies, specifically circulating tumor DNA (ctDNA), in the management of NSCLC with EGFR mutations. While tissue-based genomic testing remains the cornerstone for clinical decision-making, liquid biopsies offer a well-validated, guideline-recommended alternative approach. Ongoing trials integrating ctDNA for EGFR-mutant NSCLC management are also discussed, shedding light on the potential of ctDNA in early-stage disease, including its applications in prognostication, risk stratification, and minimal residual disease detection post-curative intent treatment. For advanced disease, the role of ctDNA in identifying resistance mechanisms to EGFR tyrosine kinase inhibitors (TKIs) is explored, providing insights into disease progression and guiding treatment decisions. This review also addresses the challenges, including the limitations in sensitivity of current assays for disease recurrence detection, and calls for future studies to refine treatment approaches, standardize reporting, and explore alternative biofluids for enhanced sensitivity. A systematic approach is crucial to address barriers to ctDNA deployment, ensuring equitable access, and facilitating its integration into routine clinical practice.
The Impact of SLC2A8 RNA Interference on Glucose Uptake and the Transcriptome of Human Trophoblast Cells
Authors: Lipka Aleksandra , Paukszto Łukasz , Kennedy Victoria C. , Tanner Amelia R. , Majewska Marta , Anthony Russell V.
Published: February 2024

While glucose is the primary fuel for fetal growth, the placenta utilizes the majority of glucose taken up from the maternal circulation. Of the facilitative glucose transporters in the placenta, SLC2A8 (GLUT8) is thought to primarily function as an intracellular glucose transporter; however, its function in trophoblast cells has not been determined. To gain insight into the function of SLC2A8 in the placenta, lentiviral-mediated RNA interference (RNAi) was performed in the human first-trimester trophoblast cell line ACH-3P. Non-targeting sequence controls (NTS RNAi; n = 4) and SLC2A8 RNAi (n = 4) infected ACH-3P cells were compared. A 79% reduction in SLC2A8 mRNA concentration was associated with an 11% reduction (p ≤ 0.05) in ACH-3P glucose uptake. NTS RNAi and SLC2A8 RNAi ACH-3P mRNA were subjected to RNAseq, identifying 1525 transcripts that were differentially expressed (|log2FC| > 1 and adjusted p-value < 0.05), with 273 transcripts derived from protein-coding genes, and the change in 10 of these mRNAs was validated by real-time qPCR. Additionally, there were 147 differentially expressed long non-coding RNAs. Functional analyses revealed differentially expressed genes involved in various metabolic pathways associated with cellular respiration, oxidative phosphorylation, and ATP synthesis. Collectively, these data indicate that SLC2A8 deficiency may impact placental uptake of glucose, but that its likely primary function in trophoblast cells is to support cellular respiration. Since the placenta oxidizes the majority of the glucose it takes up to support its own metabolic needs, impairment of SLC2A8 function could set the stage for functional placental insufficiency.
Exercise Training Differentially Affects Skeletal Muscle Mitochondria in Rats with Inherited High or Low Exercise Capacity
Authors: Heyne Estelle , Zeeb Susanne , Junker Celina , Petzinna Andreas , Schrepper Andrea , Doenst Torsten , Koch Lauren G. , Britton Steven L. , Schwarzer Michael
Published: February 2024

Exercise capacity has been related to morbidity and mortality. It consists of an inherited and an acquired part and is dependent on mitochondrial function. We assessed skeletal muscle mitochondrial function in rats with divergent inherited exercise capacity and analyzed the effect of exercise training. Female high (HCR)- and low (LCR)-capacity runners were trained with individually adapted high-intensity intervals or kept sedentary. Interfibrillar (IFM) and subsarcolemmal (SSM) mitochondria from gastrocnemius muscle were isolated and functionally assessed (age: 15 weeks). Sedentary HCR presented with higher exercise capacity than LCR paralleled by higher citrate synthase activity and IFM respiratory capacity in skeletal muscle of HCR. Exercise training increased exercise capacity in both HCR and LCR, but this was more pronounced in LCR. In addition, exercise increased skeletal muscle mitochondrial mass more in LCR. Instead, maximal respiratory capacity was increased following exercise in HCRs’ IFM only. The results suggest that differences in skeletal muscle mitochondrial subpopulations are mainly inherited. Exercise training resulted in different mitochondrial adaptations and in higher trainability of LCR. HCR primarily increased skeletal muscle mitochondrial quality while LCR increased mitochondrial quantity in response to exercise training, suggesting that inherited aerobic exercise capacity differentially affects the mitochondrial response to exercise training.
Neoadjuvant Radiochemotherapy Alters the Immune and Metabolic Microenvironment in Oral Cancer—Analyses of CD68, CD163, TGF-β1, GLUT-1 and HIF-1α Expressions
Authors: Weber Manuel , Ries Jutta , Braun Kristina , Wehrhan Falk , Distel Luitpold , Geppert Carol , Lutz Rainer , Kesting Marco , Trumet Leah
Published: February 2024

Background: The first-line treatment of oral squamous cell carcinoma (OSCC) involves surgical tumor resection, followed by adjuvant radio(chemo)therapy (R(C)T) in advanced cases. Neoadjuvant radio- and/or chemotherapy has failed to show improved survival in OSCC. Recently, neoadjuvant immunotherapy has shown promising therapeutic efficacy in phase 2 trials. In this context, the addition of radio- and chemotherapy is being reconsidered. Therefore, a better understanding of the tumor-biologic effects of neoadjuvant RCT would be beneficial. The current study was conducted on a retrospective cohort of patients who received neoadjuvant RCT for the treatment of oral cancer. The aim of the study was to evaluate the influence of neoadjuvant RCT on the immunological tumor microenvironment (TME) and hypoxic and glucose metabolisms. Methods: A cohort of 45 OSSC tissue samples from patients were analyzed before and after RCT (total 50.4 Gy; 1.8 Gy 5× weekly; Cisplatin + 5-Fluorouracil). Immunohistochemistry for CD68, CD163, TGF-β, GLUT-1 and HIF-1α was performed using tissue microarrays and automated cell counting. Differences in expression before and after RCT and associations with histomorphological parameters (T-status, N-status) were assessed using the Mann–Whitney U test. Results: Tumor resection specimens after neoadjuvant RCT showed a significant decrease in CD68 infiltration and a significant increase in CD163 cell density. The CD68/CD163 ratio was significantly lower after RCT, indicating a shift toward M2 polarization. The GLUT-1 and HIF-1α expressions were significantly lower after RCT. Larger tumors (T3/T4) showed a lower GLUT-1 expression. Other biomarkers were not associated with the T- and N-status. Conclusions: Neoadjuvant RCT with 50.4 Gy induced a shift toward the M2 polarization of macrophages in the TME. This change in immune composition is not favorable and may be prognostically negative and counteract immunotherapeutic approaches. In addition, the decreased expressions in GLUT-1 and HIF-1α indicate reductions in the glucose metabolism and hypoxic energy metabolism in response to “high dose” neoadjuvant RCT, which may be therapeutically desirable.
Immunity in the Progeroid Model of Cockayne Syndrome: Biomarkers of Pathological Aging
Authors: Zayoud Khouloud , Chikhaoui Asma , Kraoua Ichraf , Tebourbi Anis , Najjar Dorra , Ayari Saker , Safra Ines , Kraiem Imen , Turki Ilhem , Menif Samia , Yacoub-Youssef Houda
Published: February 2024

Cockayne syndrome (CS) is a rare autosomal recessive disorder that affects the DNA repair process. It is a progeroid syndrome predisposing patients to accelerated aging and to increased susceptibility to respiratory infections. Here, we studied the immune status of CS patients to determine potential biomarkers associated with pathological aging. CS patients, as well as elderly and young, healthy donors, were enrolled in this study. Complete blood counts for patients and donors were assessed, immune cell subsets were analyzed using flow cytometry, and candidate cytokines were analyzed via multi-analyte ELISArray kits. In CS patients, we noticed a high percentage of lymphocytes, an increased rate of intermediate and non-classical monocytes, and a high level of pro-inflammatory cytokine IL-8. In addition, we identified an increased rate of particular subtypes of T Lymphocyte CD8+ CD28− CD27−, which are senescent T cells. Thus, an inflammatory state was found in CS patients that is similar to that observed in the elderly donors and is associated with an immunosenescence status in both groups. This could explain the CS patients’ increased susceptibility to infections, which is partly due to an aging-associated inflammation process.
Synergistic Hepatoprotective Effects of Mesenchymal Stem Cells and Platelet-Rich Plasma in a Rat Model of Bile Duct Ligation-Induced Liver Cirrhosis
Authors: Shivaramu Shivaraju , Maiti Swapan Kumar , Banu Shajahan Amitha , Kalaiselvan Elangovan , Sharun Khan , Mishra Mamta , Mohan Divya , Palakkara Sangeetha , Kumar Sunil , Sahoo Monalisa , Hescheler Jürgen
Published: February 2024

Liver cirrhosis poses a global health challenge marked by significant prevalence and mortality. Current therapeutic options are limited by high costs and immune-mediated rejection, necessitating the exploration of innovative strategies to enhance hepatic self-rehabilitation, and counteract the underlying pathological mechanisms. We evaluated the hepatoprotective activity of rat adipose-derived mesenchymal stem cells (ADMSCs) in combination with platelet-rich plasma (PRP) and recombinant human hepatocyte growth factor (rh-HGF) on a rat model of liver fibrosis/cirrhosis induced by bile duct ligation (BDL). Treatment with PRP or rh-HGF alone did not yield significant hepatoprotection in the BDL-induced liver cirrhosis model. However, ADMSC transplantation alone exhibited the potential to alleviate impaired liver conditions. The combination of PRP and rh-HGF demonstrated superior ameliorative effects compared to either treatment alone. Notably, the combination of ADMSC + PRP or ADMSC + rh-HGF significantly enhanced hepatoprotective capacity compared to individual or combined PRP and rh-HGF therapies. Injection of ADMSC via the tail vein reduced inflammation, hepatocyte damage, and collagen deposition, improving overall liver function. This improvement was more pronounced when ADMSC was administered with PRP and rh-HGF versus monotherapy. Our study concludes that ADMSCs exert antifibrotic effects by inhibiting hepatic stellate cell proliferation, collagen synthesis, and inducing apoptosis. ADMSCs also demonstrate immune-modulatory effects and transdifferentiate into hepatic progenitor cells, secreting trophic factors, cytokines, and chemokines that promote impaired liver regeneration. The observed arrest in liver fibrosis progression highlights the potential therapeutic impact of these interventions.
miR-154-5p Is a Novel Endogenous Ligand for TLR7 Inducing Microglial Activation and Neuronal Injury
Authors: McGurran Hugo , Kumbol Victor , Krüger Christina , Wallach Thomas , Lehnardt Seija
Published: February 2024

Toll-like receptors (TLRs) are a collection of pattern recognition sensors that form a first line of defence by detecting pathogen- or damage-associated molecular patterns and initiating an inflammatory response. TLR activation in microglia, the major immune cells in the brain, can trigger the release of inflammatory molecules, which may contribute to various CNS diseases including Alzheimer’s disease. Recently, some microRNAs were shown to serve as signalling molecules for TLRs. Here, we present miR-154-5p as a novel TLR7 ligand. Exposing microglia to miR-154-5p results in cytokine release and alters expression of the TLR signalling pathway dependent on TLR7. Additionally, miR-154-5p causes neuronal injury in enriched cortical neuron cultures and additive toxicity in the presence of microglia. Finally, intrathecal injection of miR-154-5p into mice leads to neuronal injury and accumulation of microglia in the cerebral cortex dependent on TLR7 expression. In conclusion, this study establishes miR-154-5p as a direct activator of TLR7 that can cause neuroinflammation and neuronal injury, which may contribute to CNS disease.
Selective Colorimetric Detection of Pb(II) Ions by Using Green Synthesized Gold Nanoparticles with Orange Peel Extract
Authors: Zannotti Marco , Piras Sara , Remia Lorenzo , Appignanesi Diego , Giovannetti Rita
Published: February 2024

Gold nanoparticles (AuNPs) were prepared by using a green approach that employed orange (citrus sinensis) peel water extract (OPE) as a reducing agent. In this case, the organic compounds present in orange peel were able to reduce Au(III) to Au(0) and, at the same time, to act as a capping agent, functionalizing the surface of the AuNPs, stabilizing them in a water solution. This “green” approach valorizes orange peel waste as a resourceful material and makes the synthetic process of AuNPs more environmentally sustainable, safe, and economically feasible than the traditional methods. The obtained gold nanoparticles (AuNPs@OPE) were characterized by FT–IR, DLS, SEM analysis, and UV–Vis spectroscopy; the latter showed a characteristic surface plasmon resonance (SPR) band at 530 nm, typical of spherical gold nanoparticles. The AuNPs@OPE were then tested as colorimetric sensors for heavy metals in water, showing an affinity and selectivity toward Pb2+. In fact, in the presence of Pb2+, the added cation favors the aggregation process, and, in this case, nanoparticles form clusters due to the interactions between Pb2+ and the carboxyl/hydroxyl groups on the surface of the AuNPs@OPE, increasing the size of the nanostructure. This process is accompanied by a change in color of the AuNPs@OPE from pink to violet, with a formation of a second, new SPR band, at a higher wavelength, relative to the aggregate formation. The colorimetric assay was tested at different times with the addition of Pb2+ ions showing different LOD values of 13.31 µM and 0.05 µM after 15 min and 90 min, respectively. The proposed colorimetric assay was also tested for analyzing Pb2+ in drinking water samples demonstrating the reliability to use AuNPs@OPE with real samples.
Combination of Machine Learning and RGB Sensors to Quantify and Classify Water Turbidity
Authors: Parra Lorena , Ahmad Ali , Sendra Sandra , Lloret Jaime , Lorenz Pascal
Published: February 2024

Turbidity is one of the crucial parameters of water quality. Even though many commercial devices, low-cost sensors, and remote sensing data can efficiently quantify turbidity, they are not valid tools for the classification it. In this paper, we design, calibrate, and test a novel optical low-cost sensor for turbidity quantification and classification. The sensor is based on an RGB light source and a light detector. The analyzed samples are characterized by turbidity values from 0.02 to 60 NTUs, and have four different sources. These samples were generated to represent natural turbidity sources and leaves in the marine areas close to agricultural lands. The data are gathered using 64 different combinations of light, generating complex matrix data. Machine learning models are compared to analyze this data, including training, validation, and test datasets. Moreover, different alternatives for data preprocessing and feature selection are assessed. Concerning the quantification of turbidity, the best results were obtained using averaged data and principal components analyses in conjunction with exponential gaussian process regression, achieving an R2 of 0.979. Regarding the classification of the turbidity, an accuracy of 91.23% is obtained with the fine K-Nearest-Neighbor classifier. The cases in which data were misclassified are characterized by turbidity values lower than 5 NTUs. The obtained results represent an improvement over the current solutions in terms of turbidity quantification and a completely novel approach to turbidity classification.
Brugada Syndrome: Focus for the General Pediatrician
Authors: Speranzon Alessia , Chicco Daniela , Bonazza Paolo , D’Alfonso Raffaele , Bobbo Marco , D’Agata Mottolese Biancamaria , Barbi Egidio , Caiffa Thomas
Published: February 2024

Brugada Syndrome is an “inherited” channelopathy characterized by a predisposition to syncope and sudden death. It typically presents in young adults but is also known to affect the pediatric population, even if the prevalence is low compared to the adult population. The diagnostic ECG pattern shows coved-type ST-segment elevation in the right precordial leads, occurring spontaneously or after provocative drug tests with IV administration of Class I antiarrhythmic drugs. However, the electrocardiographic findings may vary, and transient or concealed forms of the syndrome further complicate diagnosis, necessitating thorough evaluation and close clinical follow-up. The clinical presentation of Brugada Syndrome may range from asymptomatic individuals to patients who have experienced syncope or sudden cardiac arrest. The syndrome remains underdiagnosed due to its elusive symptoms and the absence of abnormal findings between episodes. Additionally, specific triggers such as fever, certain medications and alcohol consumption may unmask the electrocardiographic changes and provoke arrhythmias in susceptible individuals. Given its elusive nature, early diagnosis and risk stratification are crucial in identifying individuals who may benefit from an implantable cardioverter defibrillator, the mainstay of treatment for high-risk patients, or pharmacological interventions.
Laryngomalacia and Obstructive Sleep Apnea in Children: From Diagnosis to Treatment
Authors: Cerritelli Luca , Migliorelli Andrea , Larini Alessio , Catalano Andrea , Caranti Alberto , Bianchini Chiara , Ciorba Andrea , Stomeo Francesco , Vicini Claudio , Pelucchi Stefano
Published: February 2024

The aim of this review is to investigate the state of the art among the association between Obstructive sleep apnea (OSA) and laryngomalacia, analyzing the epidemiology, the diagnostic tools, and the possible treatments available to affected patients. Laryngomalacia, characterized by the malacic consistency of the epiglottis with a tendency to collapse during inspiratory acts, producing a characteristic noise known as stridor, is a common condition in infants and particularly in those affected by prematurity, genetic diseases, craniofacial anomalies, and neurological problems. Congenital laryngomalacia, presenting with stridor within the first 15 days of life, is often self-limiting and tends to resolve by 24 months. OSA is not only a consequence of laryngomalacia but also exacerbates and perpetuates the condition. Currently, the treatments reported in the literature are based (i) on medical therapies (including watchful waiting) and (ii) on surgical treatments. Among the surgical techniques, the most described is supraglottoplasty, performed with the use of cold instruments, CO2 LASER, transoral robotic surgery, or the microdebrider.
Assessment of the (Pro)renin Receptor Protein Expression in Organs
Authors: Yang Teng-Yao , Chang Pey-Jium , Ko Yu-Shien , Shen Siou-Ru , Chang Shun-Fu
Published: February 2024

The (pro)renin receptor ((P)RR) is an essential component of the renin–angiotensin system (RAS) as a specific single-pass transmembrane receptor for prorenin and renin and has now emerged as a multifunctional protein implicated in a wide variety of developmental and physio-pathological processes and pathways. The (P)RR may be of pathological significance in metabolic syndrome. The (P)RR has received much consideration; substantial efforts have been made to understand the localization, regulation, and function of the (P)RR at both a molecular and system level. (P)RR regulation of cell function depends on whether it is intact or cleaved into its constituent forms. Therefore, the present chapter describes immunohistochemical approaches to examine the expression of (P)RR in various organs. It was shown that different molecular forms of (P)RR could be present in different tissue compartments in almost all organs. Among them, the liver has high PRR activity. Our findings could elucidate more detailed distribution of different (P)RR molecular forms in different organs, which could provide useful information to further investigate the pathophysiological mechanisms of the development of various diseases in the future.
Tackling Complexity: Integrating Responses to Internal Displacements, Extreme Climate Events, and Pandemics
Authors: Abeldaño Zuñiga Roberto Ariel , de Lima Gabriela Narcizo , Suarez-Herrera José Carlos
Published: February 2024

Background: During 2020 and 2021, over 50.2 million individuals were forced to leave their homes to escape the impacts of climate-related disasters, unable to practice social isolation or self-quarantine. A considerable proportion of them reside in densely populated areas with a lack of basic services such as water and sanitation and limited access to essential healthcare. This study aimed to estimate the internal displacements during 2020 and 2021 due to climate-related events, and review the evidence for proposing policy recommendations. Methods: Data from the Internal Displacement Monitoring Centre were used for assessing internal displacement by disasters during 2020 and 2021. In addition, the authors conducted a bibliographic review to analyse the responses to internal displacements in climate-related disasters. Results: There were 883 severe storms and 1567 flood events resulting in 50.2 million internal displacements globally. Through the documents reviewed, the legal framework, the vulnerabilities and current challenges of internally displaced persons, and the response policy recommendations were analysed. Conclusions: The increased awareness of displacement and migration, particularly driven by climate-related factors, aligns with international agreements emphasising coordinated action. This recognition becomes even more critical in the context of the convergence of climate-related displacements and the potential for future pandemics.
Historical Climate Trends and Extreme Weather Events in the Tri-State Area: A Detailed Analysis of Urban and Suburban Differences
Authors: Malikah Sameeha , Avila Stephanie , Garcia Gabriella , Lakhankar Tarendra
Published: February 2024

This study analyzes daily temperature and precipitation data collected from 44 weather stations throughout New York, New Jersey, and Connecticut to assess and quantify the historical climatic changes within these states. The study conducts a detailed examination of spatial and temporal trends, focusing on specific stations that best represent the climatic diversity of each area. A critical analysis aspect involves comparing temperature trends in urban and suburban areas, mainly focusing on New York City. The findings reveal a significant upward increasing trend in average temperatures across all seasons, with urban areas, especially NYC, exhibiting the most marked increases. This trend is notably sharp in the spring, reflecting climate change’s escalating influence. The study also observes an increase in the annual average temperatures and a concurrent decrease in the variability of temperature ranges, suggesting a stabilization of temperature fluctuations over time. Also, we identified a notable increase in heat wave frequency, more so in urban locales than in their suburban counterparts. Analysis of precipitation patterns, particularly in NYC, reveals a decline in snowfall days, consistent with the general warming trend. The results demonstrate significant trends in seasonal average temperatures, a decrease in the variability of temperatures, and a rise in heat wave occurrences, with urban areas typically experiencing warmer conditions. This comprehensive study highlights the need for a more in-depth analysis of spatial precipitation trends. It underscores the importance of continued research in understanding the multifaceted impacts of climate change, particularly in differentiating urban and rural experiences.
Design of an Azopolymer for Photo-Switchable Adhesive Applications
Authors: Siniscalco David , Pessoni Laurence , Boussonnière Anne , Castanet Anne-Sophie , Billon Laurent , Vignaud Guillaume , Delorme Nicolas
Published: February 2024

Significant research endeavors have been devoted to developing adhesives with reversible switching capabilities, allowing them to activate adhesion in response to diverse environmental stimuli. Among these, photo-switchable adhesives stand out as particularly promising. The presence of a photo-reversible solid-to-liquid transition, characterized by a transition temperature (TSL), in certain azobenzene-containing polymers offers a compelling avenue for creating such adhesives. The development of a method based on Atomic Force Microscopy to measure both the glass transition temperature (Tg) and TSL provided an opportunity to investigate the impact of various structural parameters on the solid-to-liquid transition of azopolymers. Our findings revealed that increasing the molecular weight (Mn) from 3400 to 8100 g/mol needed to achieve a highly cohesive adhesive resulted in an elevation in TSL (>10 °C), making the solid-to-liquid transition at room temperature more challenging. However, incorporating a highly flexible substituent at the para position of the azobenzene group proved effective in significantly reducing the TSL value (from 42 °C to 0 °C). This approach allows for the creation of photo-switchable adhesives with intriguing properties. We believe that our results establish a pathway toward developing a robust room-temperature photo-switchable adhesive.
Universal Short-Time Conductance Behavior Emerges between Two Adjacent Reservoirs
Authors: Granot Er’el
Published: February 2024

When a shutter, which differentiates between two adjacent particles’ reservoirs with a voltage gap, is lifted, a current emerges. In this paper, the temporal dynamics of this emerging current is analyzed. The main results are as follows: (A) the current’s prefactor in the short-time behavior is related to the long-time frequencies, by which the current converges to its equilibrium value (the conductance quantum unit 2e2/h). (B) In the short-time regime, the current is proportional to the square root of the time. (C) The maximum overshoot conductance is bounded by Gmax = ζe2/h, where ζ is a universal value which is very close to Euler’s number. (D) Most of these results are valid for a thin wire in 3D, even in the presence of electron–electron interactions.
High-Pressure Phases of Boron Pnictides BX (X = As, Sb, Bi) with Quartz Topology from First Principles
Authors: Solozhenko Vladimir L. , Matar Samir F.
Published: February 2024

The superdense hexagonal boron pnictides BX (X = As, Sb, Bi), whose structures are formed by distorted tetrahedra and characterized by a quartz-derived (qtz) topology, have been predicted from first principles as potential high-pressure phases. From full geometry structure relaxation and ground state energy calculations based on quantum density functional theory (DFT), qtz BX was found to be mechanically (elastic constants) and dynamically (phonons) stable. From the energy–volume equations of state, at high but experimentally accessible pressures, qtz boron pnictides were found to be more energetically favorable than corresponding cubic zinc–blende phases with diamond-like (dia) topology. According to the electronic band structures, the zinc–blende BX have larger band gaps than the qtz phases, which can be attributed to the higher covalence of the latter. A metallic behavior is only observed for qtz BBi, which is related to the dynamic instability as it follows from the phonon band structure.
Bryophyte Flora in Alpine Grasslands of the Qinghai–Tibet Plateau Based on Plot Sampling
Authors: Liu Yan , He Ying , Tian Yue , Zhao Zhengwu
Published: February 2024

The species number of bryophytes is the second highest among land plants. Alpine grasslands on the Qinghai–Tibet Plateau (QTP) are the largest among global alpine biomes. However, bryophyte flora in alpine grasslands on the QTP remains poorly explored relative to its large geographic extent. A total of 347 plots were surveyed across the QTP, and 149 bryophyte taxa in 24 families and 49 genera were recorded in alpine grasslands. The largest family was Pottiaceae, followed by Bryaceae and Brachytheciaceae. The most species-rich genus was Bryum, followed by Didymodon and Brachythecium. The dominant species were Didymodon tectorus, Didymodon fallax, Bryum caespiticium, Didymodon constrictus, and Didymodon ditrichoides. The Jaccard similarity indexes of bryophyte compositions between alpine meadow and alpine steppe at the family, genus, and species levels were 0.375, 0.367, and 0.282, respectively. Turf was the most common life-form (75.2%), followed by weft (16.1%) and cushion (5.4%). Endemic species to China accounted for 8.05% of the total taxa. Bryophyte diversity in alpine grasslands on the QTP is exceptional and irreplaceable. The changes in species composition and life-forms between different grassland types reflect the adaptations of bryophytes to harsh environments.
The Morphological Differentiation and Evolutionary Origins of Artemia in China
Authors: Pang Huizhong , Zheng Kaixuan , Wang Wenbo , Zheng Mingjuan , Zhang Yulong , Zhang Daochuan
Published: February 2024

Artemia is a genus of halophilic zooplanktons comprising bisexual and parthenogenetic forms, which is an important model for investigating adaption to hypersaline ecosystems. The genus Artemia in China comprises four species: A. sinica, A. tibetiana, A. franciscana and A. parthenogenetica. To investigate the evolutionary relationship of bisexual and parthenogenetic Artemia in China, we analyzed the morphometrics and phylogenetics among twenty-two geographical populations in China. We found significant morphological differentiation across different species and strains of Artemia in China, which exhibited a high level of intra-population variation. We also found overlaps in morphological characteristics between populations, which may raise challenges for the classification of Artemia species using traditional morphological methods. A. franciscana, which originated from various regions in America, was generally distributed along the Chinese coastlines through multiple human introductions. Additionally, native Asian clades split into Western and Eastern Lineages during the late Miocene due to the Himalayan orogeny. Within the Western Lineage, A. tibetiana can be grouped into three taxon units: A. tibeitiana, A. sorgeloosi and A. urmiana. We also found that the distribution and genetic structure of A. sinica were influenced by climate oscillations during the Pleistocene, which might play a pivotal role in driving the formation of parthenogenetic strains in the Eastern Lineage. Overall, our study provides new insight into invertebrate evolution under geographical and climatic impacts in hypersaline environments.
GPS Tracking Reveals the White-Tailed Eagle Haliaeetus albicilla as an Ambassador for the Natura 2000 Network
Authors: Probst Remo , Schmidt Matthias , McGrady Michael , Pichler Christian
Published: February 2024

The Natura 2000 network of protected areas is the backbone of species conservation in the European Union. We investigated whether Austrian-hatched white-tailed eagles (Haliaeetus albicilla) make particular use of this multinational network during their natal dispersal, and what habitats were of importance to the eagles. We analyzed the utilization distribution of 907,466 GPS locations from 38 dispersing white-tailed eagles using a dynamic Brownian Bridge Movement Model. Eagles ranged over a huge area of central-eastern Europe. Natura 2000 sites overlapped with 67% of the resulting 50% isopleth; i.e., a high probability of utilization of Natura 2000 areas by white-tailed eagles was found. White-tailed eagles used wetlands, waterbodies, and deciduous forests adjacent to wet habitats disproportionately often. Coniferous forests and settlements were avoided. Anthropogenically caused mortalities hardly occurred within Natura 2000 sites. Our study suggests that the Natura 2000 network is a crucial tool for conserving the white-tailed eagle. This top predator is an ambassador for the Natura 2000 idea during all life stages, and should continue to be a conservation priority of the network.
Evaluation of SARS-CoV-2 Vaccine-Induced Antibody Responses in Patients with Neuroimmunological Disorders: A Real-World Experience
Authors: Ju Hyunjin , Seok Jin Myoung , Chung Yeon Hak , Jeon Mi Young , Lee Hye Lim , Kwon Soonwook , Kim Sunyoung , Min Ju-Hong , Kim Byoung Joon
Published: February 2024

This study evaluates the antibody responses to SARS-CoV-2 vaccines in patients with neuroimmunological disorders (pwNID) who are receiving immunomodulating treatments, compared to healthy individuals. It included 25 pwNID with conditions such as optic neuritis, neuromyelitis optica spectrum disorder, multiple sclerosis, myasthenia gravis, and polymyositis, as well as 56 healthy controls. All participants had completed their full SARS-CoV-2 vaccination schedule, and their blood samples were collected within six months of their last dose. The concentration of anti-SARS-CoV-2 IgG antibodies was measured using an enzyme-linked immunosorbent assay. The results showed that pwNID had significantly lower antibody titers (58.4 ± 49.2 RU/mL) compared to healthy individuals (81.7 ± 47.3 RU/mL). This disparity persisted even after adjusting for age and the interval between the final vaccination and sample collection. A notable correlation was found between the use of immunomodulating treatments and reduced antibody levels, whereas mRNA vaccines were linked to higher antibody concentrations. The conclusion of this study is that immunomodulating treatments may reduce the effectiveness of SARS-CoV-2 vaccines in pwNID. This insight is crucial for healthcare providers in designing vaccination strategies and managing treatment plans for pwNID on immunomodulating therapies, highlighting the need for personalized approaches in this subgroup.
Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light
Authors: Wiench Rafał , Paliga Dariusz , Mertas Anna , Bobela Elżbieta , Kuśka-Kiełbratowska Anna , Bordin-Aykroyd Sonia , Kawczyk-Krupka Aleksandra , Grzech-Leśniak Kinga , Lukomska-Szymanska Monika , Lynch Edward , Skaba Dariusz
Published: February 2024

Background: Candida albicans and similar species are significant pathogens in immunocompromised and hospitalized individuals, known for mucosal colonization and bloodstream/organ invasion. Many pathogenic fungi, including these species, exhibit autofluorescence (R/OF) under specific light conditions, a feature crucial for their detection. Aim: We investigated the use of a 405 nm diode laser for the direct observation of red/orange autofluorescence of Candida spp., common in the oral cavity, exploring its potential in health screenings. Methods: This study utilized cultures of Candida spp. on Sabouraud dextrose agar with Qdot 655 and 685 for fluorescence benchmarking, illuminated using a 405 nm diode laser (continuous wave, power 250 mW, 0.0425 J/cm² fluence, 0.0014 W/cm² power density). Images were captured using a yellow-filter camera at set intervals (48 to 144 h). Visual and computational analyses evaluated the R/OF in terms of presence, intensity, coloration, and intra-colony variation. Results: Most Candida strains displayed red/orange autofluorescence at all observation times, characterized by varied coloration and intra-colony distribution. Initially, there was an increase in R/OF intensity, which then stabilized in the later stages of observation. Conclusions: The majority of the Candida strains tested are capable of emitting R/OF under 405 nm laser light. This finding opens up new possibilities for integrating R/OF detection into routine dental screenings for Candida spp.
Debris and Smear Layer Removal in Curved Root Canals: A Comparative Study of Ultrasonic and Sonic Irrigant Activation Techniques
Authors: Wigler Ronald , Srour Yara , Wilchfort Yuval , Metzger Zvi , Kfir Anda
Published: February 2024

The aim of this study was to compare the cleaning efficacy of three irrigant activation devices with a control of non-activated syringe and needle irrigation in curved root canals. Sixty human curved roots were endodontically prepared and divided into four groups (n = 15) with similar root curvature distributions. Final irrigation using 4% NaOCl was performed with a syringe and needle (30-G) alone, or with Eddy sonic powered irrigation system (polymeric tip #0.25/0.06), Endosonic ultrasonic activation (polymeric tip #0.25/0.03), or Irrisafe ultrasonic activation (stainless-steel tip, #0.25/0.00). SEM was used to evaluate cleaning efficacy, employing five-score systems for debris and smear layer. While no significant difference in debris removal was observed between Endosonic or Irrisafe activation and non-activated irrigation, Eddy sonic powered irrigation system significantly improved debris removal in the apical third of curved root canals. Smear layer removal was effective in coronal and mid-root sections for all groups but less so in the apical third. Thus, Eddy sonic powered irrigation system demonstrated higher efficacy in removing debris from the apical third of curved root canals compared with non-activated syringe and needle irrigation. However, all three irrigant activation systems exhibited no difference from the non-activated control in smear layer removal.
The Impact of a Combination of Flipped Classroom and Project-Based Learning on the Learning Motivation of University Students
Authors: Köpeczi-Bócz Tamás
Published: February 2024

Our study investigated the effects of combining project-based learning (PBL) and flipped classroom (FC) methods in university education on the learning motivation and learning outcomes of students at the bachelor (BSc), master (MSc), and higher vocational education and training (HVET) levels. We aimed to explore how these modern teaching methods can influence students’ performance and motivation at different levels of education. The research used cross-sectional analysis and quantitative methods to evaluate the impact of FC and combined PBL and FC methods. This study followed groups of students for two academic years, comparing their results with control groups that did not benefit from the combined teaching method but were studied in a same FC environment. The results showed that students at the BSc and HVET levels significantly improved their learning motivation and achievement by combining PBL and FC methods (BSc: p = 0.0001248, HVET: p = 0.0485), while at the MSc level, this effect was not significant (p = 0.1000). These data support that an effective combination of PBL and FC methods can improve learning motivation and outcomes at certain levels of education, but further research is needed better to understand the effects for students at the MSc level.
A Quadrature Oscillator with a Frequency-Tuned Distributed RC Network Analysis
Authors: Koo Jahyun , Jeon Cheonhoo
Published: February 2024

This paper introduces an innovative two-stage distributed RC oscillator design, enhancing the noise performance and frequency stability for compact electronic devices. This work significantly reduces the comparator noise and improves system reliability by implementing a novel approach to increase the signal transition slope, coupled with optimized resistor and capacitor configurations. The study employs a quadrature oscillator topology and a precise reference voltage generation method, effectively addressing the challenges of mismatch and noise performance. A 469.2 kHz quadrature oscillator with two-stage distributed RC is implemented with a 0.18 μm CMOS process, achieving a FoM of −160 dBc/Hz at 100 Hz with a stable −20 dB roll-off in the phase noise and an Allan deviation floor of less than 0.7 ppm.
Domain Adaptive Channel Pruning
Authors: Yang Ge , Zhang Chao , Gao Ling , Guo Yufei , Guo Jinyang
Published: February 2024

Domain adaptation is an effective approach to improve the generalization ability of deep learning methods, which makes a deep model more stable and robust. However, these methods often suffer from a deployment problem when deep models are deployed on different types of edge devices. In this work, we propose a new channel pruning method called Domain Adaptive Channel Pruning (DACP), which is specifically designed for the unsupervised domain adaptation task, where there is considerable data distribution mismatch between the source and the target domains. We prune the channels and adjust the weights in a layer-by-layer fashion. In contrast to the existing layer-by-layer channel pruning approaches that only consider how to reconstruct the features from the next layer, our approach aims to minimize both classification error and domain distribution mismatch. Furthermore, we propose a simple but effective approach to utilize the unlabeled data in the target domain. Our comprehensive experiments on two benchmark datasets demonstrate that our newly proposed DACP method outperforms the existing channel pruning approaches under the unsupervised domain adaptation setting.
CFD Simulation Models and Diffusion Models for Predicting Carbon Dioxide Plumes following Tank and Pipeline Ruptures—Laboratory Test and a Real-World Case Study
Authors: Abraham John , Cheng Lijing , Gorman John
Published: February 2024

Ruptures of pipelines can result in dangerous fluids spreading toward populated areas. It is critical for designers to have tools that can accurately predict whether populated areas might be within a plume rupture zone. Numerical simulations using computational fluid dynamics (CFD) are compared here with experimental and real-world carbon dioxide ruptures. The experimental data were used to validate the computer model; subsequently, the algorithm was used for a real-world rupture from 2020 that occurred in the USA. From experiments, CFD predictions were superior to diffusion model results based on measurements made downstream of the release (within 1% concentration). Results from the real-world simulation confirm that a nearby town was in a plume pathway. Citizens in the town sought medical attention consistent with the calculated plume concentrations. CFD predictions of the airborne concentration of carbon dioxide in the town approximately 1 mile (1.5 km) downstream of the rupture reveal time-averaged concentrations of ~5%. One person was unconscious for ~45 min at a distance of 0.6 miles from the rupture site; other unconscious persons were in the center of the town (~1 mile from the rupture site) and ~1.2 miles from the rupture. These reports are in excellent agreement with the calculated plume concentrations in the region.
Hydrogen-Powered Vehicles: Comparing the Powertrain Efficiency and Sustainability of Fuel Cell versus Internal Combustion Engine Cars
Authors: Durkin Kirill , Khanafer Ali , Liseau Philip , Stjernström-Eriksson Adam , Svahn Arvid , Tobiasson Linnéa , Andrade Tatiana Santos , Ehnberg Jimmy
Published: February 2024

Due to the large quantities of carbon emissions generated by the transportation sector, cleaner automotive technologies are needed aiming at a green energy transition. In this scenario, hydrogen is pointed out as a promising fuel that can be employed as the fuel of either a fuel cell or an internal combustion engine vehicle. Therefore, in this work, we propose the design and modeling of a fuel cell versus an internal combustion engine passenger car for a driving cycle. The simulation was carried out using the quasistatic simulation toolbox tool in Simulink considering the main powertrain components for each vehicle. Furthermore, a brief analysis of the carbon emissions associated with the hydrogen production method is addressed to assess the clean potential of hydrogen-powered vehicles compared to conventional fossil fuel-fueled cars. The resulting analysis has shown that the hydrogen fuel cell vehicle is almost twice as efficient compared to internal combustion engines, resulting in a lower fuel consumption of 1.05 kg-H2/100 km in the WLTP driving cycle for the fuel cell vehicle, while the combustion vehicle consumed about 1.79 kg-H2/100 km. Regarding using different hydrogen colors to fuel the vehicle, hydrogen-powered vehicles fueled with blue and grey hydrogen presented higher carbon emissions compared to petrol-powered vehicles reaching up to 2–3 times higher in the case of grey hydrogen. Thus, green hydrogen is needed as fuel to keep carbon emissions lower than conventional petrol-powered vehicles.
Strategy for Suppressing Commutation Failures in High-Voltage Direct Current Inverter Station Based on Transient Overvoltage
Authors: Zhao Dandan , He Shaoyin , Huang Hua , Han Zheng , Cui Lv , Li Yuxin
Published: February 2024

Commutation failures in high-voltage direct current (HVDC) transmission systems often occur within inverter stations, posing challenges to the safe and consistent operation of HVDC transmission projects. This paper examines data gathered from a wide-spectrum transient electromagnetic voltage monitoring apparatus. The findings uncover a notable correlation between transient AC bus voltage disturbances, stemming from faults, and the occurrence of commutation failures in HVDC transmission systems. Leveraging this correlation, we propose a commutation failure mitigation strategy for HVDC systems centered around AC bus voltage disturbances within inverter stations. Through fault validation simulations conducted across three HVDC transmission systems, our strategy demonstrates effective suppression of commutation failure incidents within HVDC transmission projects.
Effect of Cross Nanowall Surface on the Onset Time of Explosive Boiling: A Molecular Dynamics Study
Authors: Fallahzadeh Rasoul , Bozzoli Fabio , Cattani Luca , Azam Muhammad Waheed
Published: February 2024

Explosive boiling is a fast-phase transition from an ultra-thin liquid film to vapor under an extremely high heat flux, which typically has been studied using the molecular dynamics simulation (MDS) method. The present MDS study investigated the explosive boiling of a liquid argon nanofilm over different solid copper surfaces with different nanowall patterns, including parallel and cross nanowalls. For each surface, atomic motion trajectories, the number of liquid and vapor argon atoms, heat flux, and, mainly, the onset time of explosive boiling were investigated. The simulation results indicated that explosive boiling occurs earlier on parallel and cross nanowall surfaces than on an ideally smooth surface, regardless of the topology and configuration of the nanowalls. Moreover, the results revealed that by using the cross nanowall surfaces, the onset time of explosive boiling decreased by 0.7–4% compared to the parallel nanowall surfaces. In addition, it was found that the onset time of explosive boiling strongly depends on the potential energy barrier and the movement space between nanowalls for both parallel and cross nanowall surfaces. Furthermore, the simulation findings showed that even though increasing the height of cross nanowalls increases the heat flux and temperature of the fluid argon domain, it does not necessarily result in a shorter onset time for explosive boiling. These findings demonstrate the capability of cross nanowall surfaces for explosive boiling, thereby being utilized in future surface design for thermal management applications.