bluuebunny/crossref_metadata_2025 huggingface dataset:
Kisspeptin Receptor on the Sperm Surface Reflects Epididymal Maturation in the Dog
Authors: Gloria Alessia , Contri Alberto , Mele Elena , Fasano Silvia , Pierantoni Riccardo , Meccariello Rosaria
Published: September 2021

Alongside the well-known central modulatory role, the Kisspeptin system, comprising Kiss1, its cleavage products (Kisspeptins), and Kisspeptin receptor (Kiss1R), was found to regulate gonadal functions in vertebrates; however, its functional role in the male gamete and its localization during maturation have been poorly understood. The present study analyzed Kisspeptin system in dog testis and spermatozoa recovered from different segments of the epididymis, with focus on Kiss1R on sperm surface alongside the maturation during epididymal transit, demonstrated by modification in sperm kinetic, morphology, and protamination. The proteins Kiss1 and Kiss1R were detected in dog testis. The receptor Kiss1R only was detected in total protein extracts from epididymis spermatozoa, whereas dot blot revealed Kiss1 immunoreactivity in the epidydimal fluid. An increase of the Kiss1R protein on sperm surface along the length of the epididymis, with spermatozoa in the tail showing plasma membrane integrity and Kiss1R protein (p < 0.05 vs. epididymis head and body) was observed by flow cytometry and further confirmed by epifluorescence microscopy and Western blot carried on sperm membrane preparations. In parallel, during the transit in the epididymis spermatozoa significantly modified their ability to move and the pattern of motility; a progressive increase in protaminization also occurred. In conclusion, Kisspeptin system was detected in dog testis and spermatozoa. Kiss1R trafficking toward plasma membrane along the length of the epididymis and Kiss1 in epididymal fluid suggested a new functional role of the Kisspeptin system in sperm maturation and storage.
Dark Side of Cancer Therapy: Cancer Treatment-Induced Cardiopulmonary Inflammation, Fibrosis, and Immune Modulation
Authors: Boopathi Ettickan , Thangavel Chellappagounder
Published: September 2021

Advancements in cancer therapy increased the cancer free survival rates and reduced the malignant related deaths. Therapeutic options for patients with thoracic cancers include surgical intervention and the application of chemotherapy with ionizing radiation. Despite these advances, cancer therapy-related cardiopulmonary dysfunction (CTRCPD) is one of the most undesirable side effects of cancer therapy and leads to limitations to cancer treatment. Chemoradiation therapy or immunotherapy promote acute and chronic cardiopulmonary damage by inducing reactive oxygen species, DNA damage, inflammation, fibrosis, deregulation of cellular immunity, cardiopulmonary failure, and non-malignant related deaths among cancer-free patients who received cancer therapy. CTRCPD is a complex entity with multiple factors involved in this pathogenesis. Although the mechanisms of cancer therapy-induced toxicities are multifactorial, damage to the cardiac and pulmonary tissue as well as subsequent fibrosis and organ failure seem to be the underlying events. The available biomarkers and treatment options are not sufficient and efficient to detect cancer therapy-induced early asymptomatic cell fate cardiopulmonary toxicity. Therefore, application of cutting-edge multi-omics technology, such us whole-exome sequencing, DNA methylation, whole-genome sequencing, metabolomics, protein mass spectrometry and single cell transcriptomics, and 10 X spatial genomics, are warranted to identify early and late toxicity, inflammation-induced carcinogenesis response biomarkers, and cancer relapse response biomarkers. In this review, we summarize the current state of knowledge on cancer therapy-induced cardiopulmonary complications and our current understanding of the pathological and molecular consequences of cancer therapy-induced cardiopulmonary fibrosis, inflammation, immune suppression, and tumor recurrence, and possible treatment options for cancer therapy-induced cardiopulmonary toxicity.
Low Ozone Concentrations Differentially Affect the Structural and Functional Features of Non-Activated and Activated Fibroblasts In Vitro
Authors: Cisterna Barbara , Costanzo Manuela , Lacavalla Maria Assunta , Galiè Mirco , Angelini Osvaldo , Tabaracci Gabriele , Malatesta Manuela
Published: September 2021

Oxygen–ozone (O2–O3) therapy is increasingly applied as a complementary/adjuvant treatment for several diseases; however, the biological mechanisms accounting for the efficacy of low O3 concentrations need further investigations to understand the possibly multiple effects on the different cell types. In this work, we focused our attention on fibroblasts as ubiquitous connective cells playing roles in the body architecture, in the homeostasis of tissue-resident cells, and in many physiological and pathological processes. Using an established human fibroblast cell line as an in vitro model, we adopted a multimodal approach to explore a panel of cell structural and functional features, combining light and electron microscopy, Western blot analysis, real-time quantitative polymerase chain reaction, and multiplex assays for cytokines. The administration of O2–O3 gas mixtures induced multiple effects on fibroblasts, depending on their activation state: in non-activated fibroblasts, O3 stimulated proliferation, formation of cell surface protrusions, antioxidant response, and IL-6 and TGF-β1 secretion, while in LPS-activated fibroblasts, O3 stimulated only antioxidant response and cytokines secretion. Therefore, the low O3 concentrations used in this study induced activation-like responses in non-activated fibroblasts, whereas in already activated fibroblasts, the cell protective capability was potentiated.
Role of the Endocannabinoid/Endovanilloid System in the Modulation of Osteoclast Activity in Paget’s Disease of Bone
Authors: Paoletta Marco , Moretti Antimo , Liguori Sara , Di Paola Alessandra , Tortora Chiara , Argenziano Maura , Rossi Francesca , Iolascon Giovanni
Published: September 2021

The role of the endocannabinoid/endovanilloid (EC/EV) system in bone metabolism has recently received attention. Current literature evidences the modulation of osteoclasts and osteoblasts through the activation or inhibition of cannabinoid receptors in various pathological conditions with secondary involvement of bone tissue. However, this role is still unclear in primary bone diseases. Paget’s disease of the bone (PDB) could be considered a disease model for analyzing the role of the EC/EV system on osteoclasts (OCs), speculating the potential use of specific agents targeting this system for managing metabolic bone disorders. The aim of the study is to analyze OCs expression of EC/EV system in patients with PDB and to compare OCs activity between this population and healthy people. Finally, we investigate whether specific agents targeting EC/EV systems are able to modulate OCs activity in this metabolic bone disorder. We found a significant increase in cannabinoid receptor type 2 (CB2) protein expression in patients with PDB, compared to healthy controls. Moreover, we found a significant reduction in multi-nucleated tartrate-resistant acid phosphatase (TRAP)–positive OCs and resorption areas after treatment with JWH-133. CB2 could be a molecular target for reducing the activity of OCs in PDB, opening new therapeutic scenarios for the management of this condition.
The Role of lncRNAs in Rare Tumors with a Focus on HOX Transcript Antisense RNA (HOTAIR)
Authors: Liguori Giuseppina , Cerrone Margherita , De Chiara Annarosaria , Tafuto Salvatore , Tracey de Bellis Maura , Botti Gerardo , Di Bonito Maurizio , Cantile Monica
Published: September 2021

Rare cancers are identified as those with an annual incidence of fewer than 6 per 100,000 persons and includes both epithelial and stromal tumors from different anatomical areas. The advancement of analytical methods has produced an accurate molecular characterization of most human cancers, suggesting a “molecular classification” that has allowed the establishment of increasingly personalized therapeutic strategies. However, the limited availability of rare cancer samples has resulted in very few therapeutic options for these tumors, often leading to poor prognosis. Long non coding RNAs (lncRNAs) are a class of non-coding RNAs mostly involved in tumor progression and drug response. In particular, the lncRNA HOX transcript antisense RNA (HOTAIR) represents an emergent diagnostic, prognostic and predictive biomarker in many human cancers. The aim of this review is to highlight the role of HOTAIR in rare cancers, proposing it as a new biomarker usable in the management of these tumors.
Geomagnetic Field (GMF)-Dependent Modulation of Iron-Sulfur Interplay in Arabidopsis thaliana
Authors: Vigani Gianpiero , Islam Monirul , Cavallaro Viviana , Nocito Fabio F. , Maffei Massimo E.
Published: September 2021

The geomagnetic field (GMF) is an environmental factor affecting the mineral nutrient uptake of plants and a contributing factor for efficient iron (Fe) uptake in Arabidopsis seedlings. Understanding the mechanisms underlining the impact of the environment on nutrient homeostasis in plants requires disentangling the complex interactions occurring among nutrients. In this study we investigated the effect of GMF on the interplay between iron (Fe) and sulfur (S) by exposing Arabidopsis thaliana plants grown under single or combined Fe and S deficiency, to near-null magnetic field (NNMF) conditions. Mineral analysis was performed by ICP-MS and capillary electrophoresis, whereas the expression of several genes involved in Fe and S metabolism and transport was assayed by qRT-PCR. The results show that NNMF differentially affects (i) the expression of some Fe- and S-responsive genes and (ii) the concentration of metals in plants, when compared with GMF. In particular, we observed that Cu content alteration in plant roots depends on the simultaneous variation of nutrient availability (Fe and S) and MF intensity (GMF and NNMF). Under S deficiency, NNMF-exposed plants displayed variations of Cu uptake, as revealed by the expression of the SPL7 and miR408 genes, indicating that S availability is an important factor in maintaining Cu homeostasis under different MF intensities. Overall, our work suggests that the alteration of metal homeostasis induced by Fe and/or S deficiency in reduced GMF conditions impacts the ability of plants to grow and develop.
Dual-Channel Heterogeneous Graph Network for Author Name Disambiguation
Authors: Zheng Xin , Zhang Pengyu , Cui Yanjie , Du Rong , Zhang Yong
Published: September 2021

Name disambiguation has long been a significant issue in many fields, such as literature management and social analysis. In recent years, methods based on graph networks have performed well in name disambiguation, but these works have rarely used heterogeneous graphs to capture relationships between nodes. Heterogeneous graphs can extract more comprehensive relationship information so that more accurate node embedding can be learned. Therefore, a Dual-Channel Heterogeneous Graph Network is proposed to solve the name disambiguation problem. We use the heterogeneous graph network to capture various node information to ensure that our method can learn more accurate data structure information. In addition, we use fastText to extract the semantic information of the data. Then, a clustering method based on DBSCAN is used to classify academic papers by different authors into different clusters. In many experiments based on real datasets, our method achieved high accuracy, which proves its effectiveness.
Simulation and Experiment Analysis of 10 kV Flexible Grounding Device
Authors: Liu Mengxuan , Zhang Chi , Liu Kangli
Published: September 2021

The traditional 10 kV distribution network grounding system has some disadvantages, such as small grounding current and poor arc extinguishing effect, thus, hindering the detection of high-resistance grounding fault. Therefore, this paper studied the flexible grounding system consisting of small-resistance and active inverter in parallel. The control system comprises the compensation current calculation module, the fault detection module, and line protection strategy. During a single-phase grounding fault, the device is designed to inject a current of a given amplitude and phase into the neutral point to effectively suppress fault-point voltage and current and, meanwhile, quickly identifying the fault line or the busbar fault and then systematically protecting the distribution line. In addition, a large number of simulations have performed based on three grounding faults (metal, low-resistance, and high-resistance) and two modes (ungrounded and small-resistance grounding). The device can all be functional. Finally, a 400 V-level experimental prototype was built, and the experimental results are consistent with the simulation results, which can verify the effectiveness and feasibility of the flexible grounding device.
Delays in the Road Construction Projects from Risk Management Perspective
Authors: Mohajeri Borje Ghaleh Reza , Pourrostam Towhid , Mansour Sharifloo Naser , Majrouhi Sardroud Javad , Safa Ebrahim
Published: September 2021

Delays in the execution stage of road construction projects are one of the significant challenges. The incapability of finishing projects according to schedule has attracted many researchers’ attention to this issue. This study has been formed to investigate delays in road construction projects from a risk management perspective. In this study, risks have been identified by structured interviews with experts. Qualitative risk analysis by a survey of experts and quantitative risk analysis by analytical hierarchy process (AHP) technique have been performed. Research results show that financial and credit problems, lands’ funding, management problems, technical problems, and natural disasters have the highest risk among the main criteria. Among the subcriteria of the risk, incomplete funding with a weight of 0.188, gardens and land price with 0.114 are the most critical risk, and ground operations with 0.017, asphalt problems with 0.009, and accident insurance with a weight of 0.006 are the least essential risk. In the following, critical criteria analysis has been performed, and solutions to reduce or eliminate these delays in road construction projects are presented.
Colour Preference of the Deer Ked Lipoptena fortisetosa (Diptera: Hippoboscidae)
Authors: Andreani Annalisa , Rosi Marzia Cristiana , Guidi Roberto , Jafrancesco David , Farini Alessandro , Belcari Antonio , Sacchetti Patrizia
Published: September 2021

Lipoptena fortisetosa, a deer ked native to Japan, has established itself in several European countries and was recently recorded in Italy. This hippoboscid ectoparasite can develop high density populations, causing annoyance to animals and concern regarding the potential risk of transmitting pathogens to humans. No monitoring or control methods for L. fortisetosa have been applied or tested up to now. This research evaluated the possible response of L. fortisetosa winged adults to different colours as the basis for a monitoring and control strategy. In the summer of 2020, a series of six differently coloured sticky panels were randomly set as traps in a wooded area used by deer for resting. The results indicated a clear preference of the deer ked for the blue panels that caught the highest number of flies during the experimental period. Lower numbers of flies were trapped on the red, green, black, and white panels, with the yellow panels recording the fewest captures. The response clearly demonstrates that this species displays a colour preference, and that coloured traps might be useful for monitoring and limiting this biting ectoparasite in natural areas harbouring wildlife and visited by people.
Potential Role of eNOS Genetic Variants in Ischemic Heart Disease Susceptibility and Clinical Presentation
Authors: Severino Paolo , D’Amato Andrea , Prosperi Silvia , Magnocavallo Michele , Mariani Marco Valerio , Netti Lucrezia , Birtolo Lucia Ilaria , De Orchi Paolo , Chimenti Cristina , Maestrini Viviana , Miraldi Fabio , Lavalle Carlo , Caputo Viviana , Palmirotta Raffaele , Mancone Massimo , Fedele Francesco
Published: September 2021

Background: IHD is determined by an inadequate coronary blood supply to the myocardium, and endothelial dysfunction may represent one of the main pathophysiological mechanisms involved. Genetic predisposition to endothelial dysfunction has been associated with IHD and its clinical manifestation. However, studies are often confounding and inconclusive for several reasons, such as interethnic differences. Validation of results in larger cohorts and new populations is needed. The aim of this study is to evaluate the associations between the allelic variants of the eNOS rs1799983 single-nucleotide polymorphism, IHD susceptibility and its clinical presentation. Methods: A total of 362 consecutive patients with suspected myocardial ischemia were enrolled. Patients were divided into three groups: G1, coronary artery disease (CAD); G2, coronary microvascular dysfunction (CMD); and G3, a control group with anatomically and functionally normal coronary arteries. Analysis of three allelic variants, GT, GG and TT, of rs1799983 for the NOS3 gene, encoding for eNOS, was performed. Results: rs1799983_GT was significantly more expressed by the ischemic groups (G1 and G2) compared to G3. The TT variant was significantly more expressed by the G1 group, compared to the G2 group. Among ischemic patients, GT was significantly more expressed in patients with acute coronary syndrome (ACS) presentation, compared to other clinical presentations. In the multivariate analysis, the allelic variant GT was found to potentially represent an independent predictor of IHD and ACS presentation. Conclusion: The presence of the SNP rs1799983_GT, encoding for eNOS, is an independent risk factor for IHD and, remarkably, for ACS presentation, independently of cardiovascular risk factors. These results may be useful for the prediction of IHD development, particularly with an acute clinical manifestation. They may allow the early identification of patients at high risk of developing IHD with an ACS, promoting a genetic-based prevention strategy against IHD.
Impact of Short-Term Topical Steroid Therapy on Selective Laser Trabeculoplasty Efficacy
Authors: Gračner Tomaž
Published: September 2021

Background: To evaluate whether short-term use of topical steroid therapy affected the efficacy of selective laser trabeculoplasty (SLT) for primary open-glaucoma (POAG). Methods: 25 eyes of 25 patients, who used a drop of dexamethasone 0.1% 4 times a day for 7 days as post-laser therapy, formed the Steroid SLT group and 24 eyes of 24 patients, where no topical steroids or nonsteroidal anti-inflammatory agents as post-laser therapy were used, formed the No-steroid SLT group. Success was defined as an intraocular pressure (IOP) lowering exceeding 20% of pretreatment IOP. Results: The mean follow-up time was 21.24 months for the Steroid SLT group and 20.25 months for the No-steroid SLT group (p = 0.990). No significant difference was found between the two groups for mean pretreatment IOP (22.20 mmHg vs. 22.33 mmHg), and for mean IOP reductions during whole follow-up period. At all follow-up visits, the mean IOP reductions were smaller in the Steroid SLT group than in the No-steroid SLT group. At all follow-up visits, the mean percent IOP reduction was smaller in the Steroid SLT group than in the No-steroid SLT group, and such a difference was significant at 12 months (25.4% vs. 29.6%, p = 0.047) and 24 months (25.3% vs. 29.7%, p = 0.024). According to the Kaplan–Meier survival analysis, the 24-month success rate was 84% in the Steroid SLT group and 79.2% in the No-steroid SLT group, with no differences between the groups (p = 0.675). Conclusion: Short-term use of topical steroid therapy had no impact on the efficacy of SLT for POAG.
Association of Infliximab and Vedolizumab Trough Levels with Reported Rates of Adverse Events: A Cross-Sectional Study
Authors: Veisman Ido , Barzilay Oranit , Bruckmayer Liora , Haj-Natour Ola , Kopylov Uri , Eliakim Rami , Ben-Horin Shomron , Ungar Bella
Published: September 2021

Infliximab and vedolizumab are effective treatments for inflammatory bowel disease (IBD), although associated with adverse events (AE). While low or non-existent drug levels and positive antidrug antibodies have been associated with therapeutic failure, there is no clear association between higher drug levels and AE. A cross-sectional study consisting of Crohn’s disease (CD) and ulcerative colitis (UC) patients receiving infliximab or vedolizumab at the Sheba Medical Center was performed. Patients completed a questionnaire regarding AEs related to biological therapy. Serum trough levels obtained on the same day were analyzed. Objective measures of outcomes were retrieved from medical records. Questionnaires were completed by infliximab (n = 169) and vedolizumab (n = 88)-treated therapy patients. Higher infliximab levels were only numerically associated with the occurrence of at least one AE (p = 0.08). When excluding fatigue and abdominal pain, higher infliximab levels were statistically associated with the occurrence of at least one AE (p = 0.03). Vedolizumab drug levels > 18 μg/mL were also linked with the occurrence of more AEs. No specific association was observed between the increased levels of either infliximab or vedolizumab and specific AEs (neurological symptoms, upper GI symptoms, infectious complications, and musculoskeletal symptoms). As significant AEs are very rare, additional multi-center studies are required.
A Bottom-Up Review of Image Analysis Methods for Suspicious Region Detection in Mammograms
Authors: Oza Parita , Sharma Paawan , Patel Samir , Bruno Alessandro
Published: September 2021

Breast cancer is one of the most common death causes amongst women all over the world. Early detection of breast cancer plays a critical role in increasing the survival rate. Various imaging modalities, such as mammography, breast MRI, ultrasound and thermography, are used to detect breast cancer. Though there is a considerable success with mammography in biomedical imaging, detecting suspicious areas remains a challenge because, due to the manual examination and variations in shape, size, other mass morphological features, mammography accuracy changes with the density of the breast. Furthermore, going through the analysis of many mammograms per day can be a tedious task for radiologists and practitioners. One of the main objectives of biomedical imaging is to provide radiologists and practitioners with tools to help them identify all suspicious regions in a given image. Computer-aided mass detection in mammograms can serve as a second opinion tool to help radiologists avoid running into oversight errors. The scientific community has made much progress in this topic, and several approaches have been proposed along the way. Following a bottom-up narrative, this paper surveys different scientific methodologies and techniques to detect suspicious regions in mammograms spanning from methods based on low-level image features to the most recent novelties in AI-based approaches. Both theoretical and practical grounds are provided across the paper sections to highlight the pros and cons of different methodologies. The paper’s main scope is to let readers embark on a journey through a fully comprehensive description of techniques, strategies and datasets on the topic.
Glutathione S-Transferases in Marine Copepods
Authors: Lauritano Chiara , Carotenuto Ylenia , Roncalli Vittoria
Published: September 2021

The glutathione S-transferase (GST) is a complex family of phase II detoxification enzymes, known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to a wide variety of endogenous and exogenous electrophilic compounds for detoxification purposes. In marine environments, copepods are constantly exposed to multiple exogenous stressors, thus their capability of detoxification is key for survival. Full identification of the GST family in copepods has been limited only to few species. As for insects, the GST family includes a wide range of genes that, based on their cellular localization, can be divided in three classes: cytosolic, microsomal, and mitochondrial. The role of GSTs might have class-specific features, thus understanding the nature of the GST family has become crucial. This paper covers information of the GST activity in marine copepods based on studies investigating gene expression, protein content, and enzymatic activity. Using published literature and mining new publicly available transcriptomes, we characterized the multiplicity of the GST family in copepods from different orders and families, highlighting the possible role of these genes as biomarker for ocean health status monitoring.
Structural Integrity of Fixed Offshore Platforms by Incorporating Wave-in-Deck
Authors: Azman Nurul Uyun , Abu Husain Mohd Khairi , Mohd Zaki Noor Irza , Mat Soom Ezanizam , Mukhlas Nurul Azizah , Syed Ahmad Sayyid Zainal Abidin
Published: September 2021

The structural integrity of offshore platforms is affected by degradation issues such as subsidence. Subsidence involves large settlement areas, and it is one of the phenomena that may be experienced by offshore platforms throughout their lives. Compaction of the reservoir is caused by pressure reduction, which results in vertical movement of soils from the reservoir to the mud line. The impact of subsidence on platforms will lead to a gradually reduced wave crest to deck air gap (insufficient air gap) and cause wave-in-deck. The wave-in-deck load can cause significant damage to deck structures, and it may cause the collapse of the entire platform. This study aims to investigate the impact of wave-in-deck load on structure response for fixed offshore structure. The conventional run of pushover analysis only considers the 100-year design crest height for the ultimate collapse. The wave height at collapse is calculated using a limit state equation for the probabilistic model that may give a different result. It is crucial to ensure that the reserve strength ratio (RSR) is not overly estimated, hence giving a false impression of the value. This study is performed to quantify the wave-in-deck load effects based on the revised RSR. As part of the analysis, the Ultimate Strength for Offshore Structures (USFOS) software and wave-in-deck calculation recommended by the International Organization for Standardization (ISO) as practised in the industry is adopted to complete the study. As expected, the new revised RSR with the inclusion of wave-in-deck load is lower and, hence, increases the probability of failure (POF) of the platform. The accuracy and effectiveness of this method will assist the industry, especially operators, for decision making and, more specifically, in outlining the action items as part of their business risk management.
Generalized Form of the Invariant Imbedding Method and Its Application to the Study of Back-Scattering in Shallow-Water Acoustics
Authors: Kazak Mikhail , Koshel Konstantin , Petrov Pavel
Published: September 2021

A generalized form of the matrix-invariant imbedding method was developed to solve boundary-value problems for coupled systems of Helmholtz-type equations. Within this approach, a boundary-value problem solution can be obtained by solving evolutionary first-order imbedding equations for a matrix-valued function. The proposed method is applied to the solution of coupled equations for mode amplitudes describing the propagation of acoustic waves in a range-dependent shallow-water waveguide. The back-scattering of modes by bathymetry features is investigated, and the coefficients of the modal expansion of the wave reflected by an inhomogeneity in the bottom relief are computed. It is demonstrated that back-scattering is strongly connected with the modal interactions and that the back-scattered field consists of modes with numbers different from the number of the incident mode.
Current Perspectives of Biocontrol Agents for Management of Fusarium verticillioides and Its Fumonisin in Cereals—A Review
Authors: N Deepa , Achar Premila N. , Sreenivasa Marikunte Y.
Published: September 2021

Fusarium verticillioides is the most predominant fungal phytopathogen of cereals and it is posing great concern from a global perspective. The fungus is mainly associated with maize, rice, sorghum, wheat, sugarcane, banana, and asparagus and causes cob, stalk, ear, root, crown, top, and foot rot. F. verticillioides produces fumonisins as the major secondary metabolite along with trace levels of beauvericin, fusaric acid, fusarin C, gibberiliformin, and moniliformin. Being a potential carcinogen, fumonisins continue to receive major attention as they are common contaminants in cereals and its processed food products. The importance of elimination of F. verticillioides growth and its associated fumonisin from cereals cannot be overemphasized considering the significant health hazards associated with its consumption. Physical and chemical approaches have been shown to reduce fumonisin B1 concentrations among feeds and food products but have proved to be ineffective during the production process. Hence, biological control methods using microorganisms, plant extracts, antioxidants, essential oils, phenolic compounds, and other advanced technologies such as growing disease-resistant crops by applying genetic engineering, have become an effective alternative for managing F. verticillioides and its toxin. The different methods, challenges, and concerns regarding the biocontrol of F. verticillioides and production of fumonisin B1 have been addressed in the present review.
Weather-Based Predictive Modeling of Cercospora beticola Infection Events in Sugar Beet in Belgium
Authors: El Jarroudi Moussa , Chairi Fadia , Kouadio Louis , Antoons Kathleen , Sallah Abdoul-Hamid Mohamed , Fettweis Xavier
Published: September 2021

Cercospora leaf spot (CLS; caused by Cercospora beticola Sacc.) is the most widespread and damaging foliar disease of sugar beet. Early assessments of CLS risk are thus pivotal to the success of disease management and farm profitability. In this study, we propose a weather-based modelling approach for predicting infection by C. beticola in sugar beet fields in Belgium. Based on reported weather conditions favoring CLS epidemics and the climate patterns across Belgian sugar beet-growing regions during the critical infection period (June to August), optimum weather conditions conducive to CLS were first identified. Subsequently, 14 models differing according to the combined thresholds of air temperature (T), relative humidity (RH), and rainfall (R) being met simultaneously over uninterrupted hours were evaluated using data collected during the 2018 to 2020 cropping seasons at 13 different sites. Individual model performance was based on the probability of detection (POD), the critical success index (CSI), and the false alarm ratio (FAR). Three models (i.e., M1, M2 and M3) were outstanding in the testing phase of all models. They exhibited similar performance in predicting CLS infection events at the study sites in the independent validation phase; in most cases, the POD, CSI, and FAR values were ≥84%, ≥78%, and ≤15%, respectively. Thus, a combination of uninterrupted rainy conditions during the four hours preceding a likely start of an infection event, RH > 90% during the first four hours and RH > 60% during the following 9 h, daytime T > 16 °C and nighttime T > 10 °C, were the most conducive to CLS development. Integrating such weather-based models within a decision support tool determining fungicide spray application can be a sound basis to protect sugar beet plants against C. beticola, while ensuring fungicides are applied only when needed throughout the season.
Intake of Koji Amazake Improves Defecation Frequency in Healthy Adults
Authors: Kurahashi Atsushi , Enomoto Toshihiko , Oguro Yoshifumi , Kojima-Nakamura Ayana , Kodaira Kazuya , Watanabe Kenichi , Ozaki Nobuhiro , Goto Hiroshi , Hirayama Masao
Published: September 2021

Reportedly, the intake of koji amazake, a beverage made from steamed rice fermented by Aspergillus oryzae, improves defecation frequency. However, its functional ingredients and mechanism of action remain unclear. To compare the effects of koji amazake and a placebo beverage on defecation frequency and to identify the functional ingredients and mechanism of action, a randomized, placebo-controlled, double-blind parallel-group comparative trial was performed on two groups. The koji amazake had 302 ± 15.5 mg/118 g of A. oryzae cells, which was not in the placebo. Compared with the placebo group, the koji amazake group showed a significant increase in weekly defecation frequency at 2 weeks (5.09 days vs. 4.14 days), 3 weeks (5.41 days vs. 4.18 days), and 4 weeks (5.09 days vs. 3.95 days), along with an increase in the weekly fecal weight at 4 weeks (724 g vs. 501 g). The intake of koji amazake did not induce significant intergroup differences in the fecal SCFA concentration, whereas it significantly decreased the relative abundance of Blautia and significantly increased that of Bacteroides at 3 weeks. Therefore, koji amazake intake improved defecation frequency, and A. oryzae cells played potentially important roles as functional ingredients.
Mergers and Acquisitions Risk Modeling
Authors: Vertakova Yulia , Vselenskaya Inga , Plotnikov Vladimir
Published: September 2021

In the context of the dynamics of the modern external environment, the importance of risk management in general and the risks inherent in the processes of mergers and acquisitions has sharply increased. This is becoming one of the primary challenges in business, the solution of which will contribute to economic growth and development. In this article, based on a broad review of literature, the key risks of mergers and acquisitions are identified and classified, the level of their significance is assessed, the relevant management tools are selected for each risk and a computer program is developed that implements the selection of tools for each specific merger and acquisition transaction. A comprehensive automated methodology for the selection of risk management tools in the implementation of mergers and acquisitions can become an effective risk management tool for companies participating in such transactions. This will allow to identify and track risks in a timely manner, assess their significance, and, among other things, contribute to the adoption of effective management decisions regarding risk management.
A New Indicator to Better Represent the Impact of Landscape Pattern Change on Basin Soil Erosion and Sediment Yield in the Upper Reach of Ganjiang, China
Authors: Zhang Yongfen , Wang Nong , Tang Chongjun , Zhang Shiqiang , Song Yuejun , Liao Kaitao , Nie Xiaofei
Published: September 2021

Landscape patterns are a result of the combined action of natural and social factors. Quantifying the relationships between landscape pattern changes, soil erosion, and sediment yield in river basins can provide regulators with a foundation for decision-making. Many studies have investigated how land-use changes and the resulting landscape patterns affect soil erosion in river basins. However, studies examining the effects of terrain, rainfall, soil erodibility, and vegetation cover factors on soil erosion and sediment yield from a landscape pattern perspective remain limited. In this paper, the upper Ganjiang Basin was used as the study area, and the amount of soil erosion and the amount of sediment yield in this basin were first simulated using a hydrological model. The simulated values were then validated. On this basis, new landscape metrics were established through the addition of factors from the revised universal soil loss equation to the land-use pattern. Five combinations of landscape metrics were chosen, and the interactions between the landscape metrics in each combination and their effects on soil erosion and sediment yield in the river basin were examined. The results showed that there were highly similar correlations between the area metrics, between the fragmentation metrics, between the spatial structure metrics, and between the evenness metrics across all the combinations, while the correlations between the shape metrics in Combination 1 (only land use in each year) differed notably from those in the other combinations. The new landscape indicator established based on Combination 4, which integrated the land-use pattern and the terrain, soil erodibility, and rainfall erosivity factors, were the most significantly correlated with the soil erosion and sediment yield of the river basin. Finally, partial least-squares regression models for the soil erosion and sediment yield of the river basin were established based on the five landscape metrics with the highest variable importance in projection scores selected from Combination 4. The results of this study provide a simple approach for quantitatively assessing soil erosion in other river basins for which detailed observation data are lacking.
Territories of Faith: 1000 Years of Landscape Multifunctionality in Santa Mariña de Augas Santas (NW Spain)
Authors: Ferro-Vázquez Cruz , Blanco-Rotea Rebeca , Sanjurjo-Sánchez Jorge , García-Rodríguez Sonia , García Quintela Marco
Published: September 2021

Landscape multifunctionality is increasingly recognized as an important aspect in sustainability and developmental debates. Yet, how and why a multifunctional landscape configuration develops over time has not been sufficiently studied. Here we present the geoarchaeological investigation of the Santa Mariña de Augas Santas site, in northwestern Spain. We focus on the role of religious practice, and of its interplay with productive strategies, in landscape transformation. A geochemical, mineralogical, and geochronological characterization of the pedo-sedimentary record (including XRF, EA-IRMS, XRD, OSL and 14C measurements) allowed to characterize catchment scale sedimentation processes in relation to agricultural activities. The geographical and chronological coincidence of production functions with documented religious activities demonstrate that both aspects shared geographical spaces during the last millennium. Current landscape multifunctionality at Santa Mariña is thus not the final outcome of a specific evolution, but an essential aspect of traditional land use strategies through history and a driver of change. This work highlights the need of a long-term study of the processes of landscape configuration when assessing the sustainability of traditional productive systems.
Electrochemical Noise Measurement to Assess Corrosion of Steel Reinforcement in Concrete
Authors: Mills Douglas , Lambert Paul , Yang Shengming
Published: September 2021

The electrochemical noise method (ENM) has previously been employed to monitor the corrosion of steel reinforcement in concrete. The development of solid-state Ag/AgCl-based probes and dedicated monitoring technology (ProCoMeter) now offers a wider range of ENM configurations. The present study involves the laboratory investigation of three mortar samples containing steel bars and varying additions of chloride, with a view to future field application. ENM could be used to provide corrosion information on reinforcement without the need to provide direct electrical connections to the steel and without the risk or inducing or increasing corrosion. In addition to half-cell potentials, measurements were made using ENM in three different probe configurations over a total test period of 90 days. The samples were then broken open and the bars extracted and cleaned. A comparison was then made between the calculated metal thickness loss obtained from the Rn values and the actual metal thickness loss. The results showed that each configuration was able to order the results in the expected manner, with the simple single substrate (SSS) arrangement providing the best correlation with direct measurements. The study is ongoing with the intention of measurements being obtained in situ on existing reinforced concrete structures.
Investigations of Structural and Electrical Properties of ALD Films Formed with the Ozone Precursor
Authors: Seweryn Aleksandra , Lawniczak-Jablonska Krystyna , Kuzmiuk Piotr , Gieraltowska Sylwia , Godlewski Marek , Mroczynski Robert
Published: September 2021

The continuous development of ALD thin films demands ongoing improvements and changes toward fabricating materials with tailored properties that are suitable for different practical applications. Ozone has been recently established as a precursor, with distinct advantages over the alternative oxidizing precursors in the ALDs of advanced dielectric films. This study reports alumina (Al2O3) and hafnia (HfO2) formation using an O3 source and compares the obtained structural and electrical properties. The performed structural examinations of ozone-based materials proved homogenous high-k films with less vacancy levels compared to water-based films. The enhanced structural properties also result in the problematic incorporation of different dopants through the bulk layer. Furthermore, analysis of electrical characteristics of the MIS structures with ALD gate dielectrics demonstrated the improved quality and good insulating properties of ozone-based films. However, further optimization of the ALD technique with ozone is needed as a relatively low relative permittivity characterizes the ultra-thin films.
Scalable Synthesis and Electrochemical Properties of Porous Si-CoSi2-C Composites as an Anode for Li-ion Batteries
Authors: Seo Hyungeun , Yang Hae-Ri , Yang Youngmo , Kim Kyungbae , Kim Sung Hyon , Lee Hyunseung , Kim Jae-Hun
Published: September 2021

Si-based anodes for Li-ion batteries (LIBs) are considered to be an attractive alternative to graphite due to their higher capacity, but they have low electrical conductivity and degrade mechanically during cycling. In the current study, we report on a mass-producible porous Si-CoSi2-C composite as a high-capacity anode material for LIBs. The composite was synthesized with two-step milling followed by a simple chemical etching process. The material conversion and porous structure were characterized by X-ray diffraction, X-ray photoelectron spectroscopy, and electron microscopy. The electrochemical test results demonstrated that the Si-CoSi2-C composite electrode exhibits greatly improved cycle and rate performance compared with conventional Si-C composite electrodes. These results can be ascribed to the role of CoSi2 and inside pores. The CoSi2 synthesized in situ during high-energy mechanical milling can be well attached to the Si; its conductive phase can increase electrical connection with the carbon matrix and the Cu current collectors; and it can accommodate Si volume changes during cycling. The proposed synthesis strategy can provide a facile and cost-effective method to produce Si-based materials for commercial LIB anodes.
Nanocellulose Based Filtration Membrane in Industrial Waste Water Treatment: A Review
Authors: Liu Yunxia , Liu Honghai , Shen Zhongrong
Published: September 2021

In the field of industrial wastewater treatment, membrane separation technology, as an emerging separation technology, compared with traditional separation technology such as precipitation, adsorption, and ion exchange, has advantages in separation efficiency, low energy consumption, low cost, simple operation, and no secondary pollution. The application has been expanding in recent years, but membrane fouling and other problems have seriously restricted the development of membrane technology. Natural cellulose is one of the most abundant resources in nature. In addition, nanocellulose has characteristics of high strength and specific surface area, surface activity groups, as well as being pollution-free and renewable, giving it a very wide development prospect in many fields, including membrane separation technology. This paper reviews the current status of nanocellulose filtration membrane, combs the widespread types of nanocellulose and its derivatives, and summarizes the current application of cellulose in membrane separation. In addition, for the purpose of nanocellulose filtration membrane in wastewater treatment, nanocellulose membranes are divided into two categories according to the role in filtration membrane: the application of nanocellulose as membrane matrix material and as a modified additive in composite membrane in wastewater treatment. Finally, the advantages and disadvantages of inorganic ceramic filtrations and nanocellulose filtrations are compared, and the application trend of nanocellulose in the filtration membrane direction is summarized and discussed.
Flexural Behavior of Ultra-High-Performance Fiber-Reinforced Concrete Beams after Exposure to High Temperatures
Authors: Chen How-Ji , Chen Chien-Chuan , Lin Hung-Shan , Lin Shu-Ken , Tang Chao-Wei
Published: September 2021

Due to the dense structure of ultra-high-performance concrete (UHPC), it is prone to explosive spalling at high temperatures. In this paper, flexural testing of UHPC and high-strength concrete (HSC) beams was carried out at room temperature and after being subjected to different levels of thermal exposure (300–500 °C). The cross-section of the beam specimen was 150 (width) × 200 (depth) mm, and its length was 1500 mm. The flexural and shear design of the beam specimens were carried out in accordance with the ACI 318M-14 code. All of the beams were singly reinforced with two #4 rebars (minimum reinforcement ratio) as a longitudinal tensile reinforcement at the bottom of the specimen and at an effective depth of 165 mm. The flexural load was applied using the three-point load method. The results show that, at room temperature and after being subjected to different thermal exposures, compared with the HSC specimens, the stiffness of the UHPC specimens in the post-cracking stage was relatively larger and the deflection under a given load was smaller. Moreover, whether at room temperature or after exposure to different thermal exposures, the ductility of the UHPC specimens was better than that of the HSC specimens.
A Coupled Eulerian-Lagrangian Simulation and Tool Optimization for Belt Punching Process with a Single Cutting Edge
Authors: Wojtkowiak Dominik , Talaśka Krzysztof , Wilczyński Dominik , Górecki Jan , Wałęsa Krzysztof
Published: September 2021

The objective of this paper is to analyze the belt punching process with the use of a single cutting edge and discuss the influence of geometrical features of the piercing punch on the perforation force. Two basic shapes of the piercing punch with a single cutting edge were tested: tools with the blade pointing inside or pointing outside. The analytical models of the stress distribution in the shearing cross sections were derived for both punches. The presented model, along with the series of empirical tests and Coupled Eulerian-Lagrangian simulation, was used for finding the effective geometry of the piercing punch with a single cutting edge for the belt perforation. The geometrical parameters taken into consideration for the tool optimization were the following: angle of the blade, thickness of the wall and diameter of the piercing punch cutting edge. The obtained results show that changing these parameters has a significant influence on the perforation force necessary to execute the machining process and affects the quality of the holes in the perforated belts. The most important geometrical features of the hollow sharpened punch are the angle and the direction of the blade, which change the force distribution and, as a result, the mechanics of the process.