bluuebunny/crossref_metadata_2025 huggingface dataset:
Status Hukum Pakaian Laundry dalam Konsep Thaharah sebagai Penyempurna Ibadah
Authors: Maulida Maulida , Muslimah Muslimah
Published: August 2022

Penelitian ini bertujuan untuk membahas tentang status hukum pakaian laundry dalam konsep thaharah sebagai penyempurna ibadah, menggunakan pendekatan kualitatif dengan metode penelitian kepustakaan. Penelitian ini dilakukan dengan melihat fenomena yang diteliti tanpa melihat intervensi. Hasil dari penelitian ini diketahui bahwa kebersihan dan kesucian diri dan tempat sekitar merupakan kunci diterimanya suatu amal ibadah bagi seorang muslim. Sebagai upaya agar tetap bersih dan suci di zaman yang serba semakin canggih sekarang ini, harus bisa menyesuaikan diri dengan perkembangannya, tanpa melalaikan syariat-syariat yang terkandung dalam ajaran Islam. Berdasarkan pada pemahaman-pemahaman menurut mazhab Syafi’i, bahwa ketika akan mencuci pakaian hendaknya para pengguna mesin cuci atau usahawan laundry memilah-memilih mana pakaian yang terdapat najis berat (berwujud) dan mana yang tidak, mendahulukan mencuci yang bernajis ringan; dan menyiram dengan air mengalir sebelum dikeringkan.

Kata Kunci: Thaharah dalam Ibadah, Hukum Pakain Laundry, Konsep Ibadah
Implementasi Desain Pendidikan Islam Di SMA/MA Persfektif Ilmu Pendidikan Islam
Authors: Zainab Khoirunnisa Shidqiyyah
Published: August 2022

This journal is about the Design of Islamics high school. Where high school is a final education school, the dicussion is not far from a period of transition or transition from children to adults. The psychological condition of adolescents is experiencing shocks and conflicts that they themselves do not understand. In adolescence physical growth is very fast and mismatched, resulting in increased psychological growth. The mental condition of adolescents becomes shaky, emotionally unstable, and sensitive to external stimuli. Like adolescence like in a rocking bridge, teenagers may fall into steep ravines and be swept by swift currents. Like plants, teenagers are plants that are developing and flowering. A child will definitely go through the toddler, teenage phase, and then become an adult. A worrying reality lately is the courage of some teenagers to commit sexual violations. There are even teenagers who argue, that the relationship between women and men does not need to be limited and does not need to be controlled by parents. From such a phenomenon, one of the tools that can avoid the occurrence of adolescent problems, both self-troubling, parents and society in general is a steady and steady Islamic religious education, as well as the development of a healthy personality and noble character. Because with a strong religious education, their hearts become calm and turbulent emotions can be controlled. In thispaper, the discussions that will be discussed include Islamic Education, Characteristics of Participants, Programs, Islamic learning processes, and Evaluation. The method used in this study uses analytical methods, types of research, and research data sources. The data analysis method used is by using descriptive methods, namely a method that examines a condition, a system of thought or an event that occurs in the present. This type of research belongs to the type of library research, namely research activities on existing data andliterature

Keywords : Islamic Education, Islamic Education Program, Learning Process of Islamic Education
Participation Model of Society in Education at Islamic Elementary School
Authors: Budianur Budianur , Setiawan Andi
Published: August 2022

This Study aims to examine the influence of society on madrasah as a social educational institution, the purpose of madrasah, programs, and the needs of organizing education. Instead the community has a responsibility to contribute resources in the relationship. This study used descriptive qualitative research approach, with library research method, Conducted by collecting, sorting, studying, analyzing materials relevant to the study, namely about school based management. This research found that the participation of the community and parents of students in the implementation of education is referred to as the participation process. The process of participation or participation of the community and parents of students in the school environment is for input making,decisions and gathering of support that requires preparation so that national development such as education can be encouraged and improve the quality of schools. In an effort to involve the participation of the community and parents of students, the school also needs to pay attention to the needs of these parties as a positive responsibility so as to create a good cooperative environment.

Keywords: Participation Mode of Society, Islamic Elementary School, Organizing Education
Pemikiran Pendidikan Ki Hajar Dewantara dan Relevansinya Dengan Pendidikan Agama Islam Multikultural
Authors: Rodin Rhoni , Huda Miftahul
Published: August 2022

This paper aims to analyze the thoughts of Ki Hajar Dewantara's education and its relevance to Multicultural Islamic Education. This type of research is descriptive qualitative. Literature study with an emphasis on the notion of peeling, summarizing and collecting a literature, then the authors provide an analysis of the data that has been collected. The results showed that there were five principles of Ki Hajar Dewantara's education, namely 1) the principle of independence, 2), the principle of nationality, 3) the principle of humanity, 4) the principle of culture, and 5) the principle of natural nature. Then Ki Hajar Dewantara stated his attitude in regulating religious education in the student park as follows: a) The religion of each student and teacher is free, mutual respect; b) Religion is included as ethnicity (character); and c) In areas where the inhabitants live according to Islamic customs, it is permissible to give religious instruction during class hours but not by force. (Holidays according to their respective religions, for the Taman college students the majority of students use their religious customs). This shows that there is a relevance between Ki Hajar Dewantara's educational thoughts on Multicultural Islamic Education.

Keywords: Ki Hajar Dewantara's Educational Thought, Multicultural Islamic Education
Upaya Meningkatkan Prestasi Belajar PAI Melalui Model Snowball Throwing Materi Kisah Nabi Muhammad SAW Pada Peserta Didik Kelas VII SMP Negeri 10 Buton Tengah
Authors: Septianingsih Iin
Published: August 2022

Pentingnya PAI disekolah adalah untuk membina dan mengasuh peserta didik agar senantiasa dapat memahami ajaran Islam secara menyeluruh. PAI bertujuan untuk meningkatkan keimanan, pemahaman, penghayatan dan pengalaman peserta didik tentang agama Islam, sehingga menjadi manusia muslim yang beriman dan bertaqwa kepada Allah SWT serta berakhlak mulia dalam kehidupan pribadi, bermasyarakat, berbangsa dan bernegara. Dalam pelaksanaannya selama ini masih ditekankan pada metode ceramah dan hafalan, sebaiknya dapat dikemas dengan baik sehingga menjadi menarik bagi peserta didik dalam memaknai ilmu yang diberikan serta mampu diamalkan dalam kehidupan sehari-hari. Salah satu model pembelajaran yang menarik adalah model snowball throwing. Hasil penelitian menunjukan bahwa model pembelajaran snowball throwing kisah Nabi Muhammad saw, terbukti dapat meningkatkan prestasi belajar peserta didik Kelas VII SMP Negeri 10 Buton Tengah. Hal ini dibuktikan dengan meningkatnya Kriteria Ketuntasan Klasikal pada siklus I yaitu sebesar 67% menjadi 87% pada siklus II. Adapun kendala-kendala yang dihadapi dalam model pembelajaran snowball throwing adalah (1) Sangat bergantung pada kemampuan peserta didik dalam mengingat materi pelajaran (2) Memerlukan waktu yang panjang untuk berdiskusi
Analisis Pembelajaran Online Pada Saat Pandemi Covid-19 di STAI Al Furqan Makassar
Authors: Asykur Muamar , Kaharuddin Kaharuddin , Ridwansyah Muh
Published: August 2022

Penelitian ini bertujuan untuk mengetahui bagaimana evaluasi pelaksanaan pembelajaran online pada saat masa pandemi. Covid 19 di STAI Al Furqan Makassar Penelitian ini adalah penelitian kuantitatif dengan desain deskriptif online survey. Populasinya mahasiswa Prodi S1 di STAI Al Furqan Makassar. yang mengikuti pembelajaran online dan bersedia mengisi google-form sejumlah  80 mahasiswa. Hasil penelitian adalah bahwa sebagian besar mahasiswa merasa tidak nyaman dengan pembelajaran online (65,2%), sebagian besar mahasiswa memilih pembelajaran dengan media non audio visual (54,3%), hampir sebagian mahasiswa memilih pembelajaran dengan media tatap layar atau online (31,5%) dan hampir sebagian mahasiswa menginginkan cara pembelajaran yang interaktif (31,5%).
The Effect of Online Games on Changes in Student Behavior in Middle Schools
Authors: Hanafie Nurharsya Khaer , Bakhtiar Bakhtiar , Darmawati Darmawati
Published: August 2022

The Effect of Online Games on Changes in Student Behavior in Junior High Schools The approach used in this study is an associative approach with quantitative research. The data collection techniques used observation, questionnaires/questionnaires and documentation. While the data analysis used simple linear regression test, Product Moment Correlation test, partial test (t), and the determinant coefficient. The results of this study indicate that: (1) Online games affect changes in student behavior in junior high schools, this is indicated by the results of the t-test analysis or partial test. The t-test or partial test is a statistic used to test the truth or falsity of the null hypothesis. So the results of statistical test analysis are known that there is a significant influence between the online game variable (X) and the student behavior change variable (Y). This means that the higher the intensity of playing online games played by students, the changes in student behavior are increasing/bad. The indicator factors for online game variables are the most dominant influencing changes in student behavior. namely online games are only used unilaterally, not balanced with valuable activities, so that they only have a negative impact and cause bad effects for students.

Keywords: Effect Online Games, Students Behavior, Online Games
Analysis of Effect Application of Stad-Type Cooperative Learning Model on Learning Outcomes at Elementary School
Authors: Nureva Nureva
Published: August 2022

This study aims to determine the Influence of Cooperative Learning Model Student Team Achievement Division (STAD) Toward Learning Outcomes IPS Class V SDN 63 Gedong Tataan Pesawaran District Subdistrict.This research is using research design pretest-posttes group design. The independent variables in this study is a cooperative learning model STAD while the dependent variable is the result of learning. The data collection technique used is the test, and documentation. The data analysis technique consists of three stages, namely, stage description of the data, the test phase of requirements analysis and hypothesis testing phase.step description of the data include data distribution learning outcomes. Test requirements analysis phase includes tests of normality and homogeneity. While the hypothesis testing phase using T-test analysis results of data analysis showed> ie 5.37> 1.68 to 0.05 level signitifkan.this proves that rejected or accepted, which means there is influence between STAD cooperative learning on the results of social studies class V SDN 63 Gedong Tataan District of Gedong Tataan Pesawaran District.

Keywords: Cooperative Learning, Learning Outcome, STAD-Type Cooperative Learning
Pengaruh Kuat Arus Listrik Terhadap Kekuatan Bending Akar Las dan Kekerasan Pada Pengelasan SMAW
Authors: Samhuddin Samhuddin , Aminur Aminur , Imran Al Ichlas , Ali Sartirlan
Published: August 2022

Struktur sambungan pada konstruksi baja yang rigid tidak terlepas dari pengelasan. Tujuan penelitian ini adalah untuk mengetahui pengaruh kuat arus terhadap kekuatan bending pada akar las dan kekerasan pada daerah logam las, base metal dan Heat Affected Zone. Bahan yang digunakan adalah plat baja karbon rendah tebal 8 mm. Pada kedua bagian sisi ujung yang disambung masing-masing dibuat groove sudut 30 derajat yang membentuk sebuah kampuh V sudut 60 derajat. Pada kampuh V dilas dengan proses SMAW posisi 1G dengan variasi arus pengelasan 70 A, 90 A dan 110 A dengan elektroda berdiamter 2,6 mm. Hasil penelitian didapatkan bahwa kekuatan bending terendah 23,20 kg/mm2 pada arus 70 A dan tertinggi 26,82 kg/mm2. Kekerasan tertinggi 192,40 kg/mm2 pada daerah logam las 110 A.Kata kunci: Baja Karbon rendah, SMAW, kampuh V, kekuatan bending dan kekerasan.
Analisa Daya Keluaran pada Generator 455 kVA untuk Produksi Batubara menggunakan Barge Loading Conveyor Di PT. Duta Tambang Rekayasa
Authors: Agustira Bayu , Hidayat Rahmat , Nurpulaela Lela
Published: August 2022

Generator adalah suatu alat berupa mesin yang dapat digunakan untuk menghasilkan tenaga listrik dari sumber energi mekanik dengan melalui proses induksi elektromagnetik. Generator ini mendapat energi mekanik dari mesin diesel yang ada didalam generator. Metode yang dilakukan pada penelitian ini menggunakan 2 metode diantaranya metode observasi yaitu adalah metode yang digunakan peneliti dengan melakukan pengamatan secara langsung terhadap objek penelitian di PT. Duta Tambang Rekayasa kemudian mencatat data-data yang didapatkan yang nantinya akan dilakukan analisa. Dapat diketahui nilai rating kinerja genset yaitu sebesar 185,9375 kVA sehingga genset yang digunakan pada PT. Duta Tambang rekayasa yaitu sebesar 455 kVA, 3P, 380 V, 50 Hz dan faktor daya atau cos ∅ 0,8. Prinsip kerja dari genset adalah sebuah mesin diesel yang bergerak dengan menhubah energi bahan bakar fosil menjadi energi mekanik, kemudian energi mekanik tersebut akan diubah oleh generator yang nantinya akan menghasilkan energi listrik.
PERANCANGAN SISTEM INFORMASI MUBALIGH BINTALDAM XIV/HN VERSI MOBILE
Authors: Rokhman Abdul , Herlinah Herlinah , Hazriani Hazriani , Nur Adnan
Published: August 2022

Pemanfaatan teknologi telpon genggam dalam dunia da’wah seharusnya bukan hanya sebatas pada penggunaaan  konunikasi personal, akan tetapi lebih lanjut harus dimanfaatkan untuk  pengembangan pelayanan informsi yang lebih luas, oleh karena itu perlu drancang sistem informasi mubaligh. Metode penelitian dalam rangka merancang sistem informasi mubaligh ini adalah menggunakan metode action research, yaitu mengembangkan sistem yang ada dengan sistem baru yang lebih baik. Metode ini didukung dengan peralatan mobile phone berbasis web dan pengumpulan datanya menggunakan pengumpulan dokumen, pengamatan, wawancara dan kuisioner. Hasil perancangan menunjukkan bahwa sistem informasi mubaligh ini dapat diakses melalui handphone dengan berbagai macam informasi tentang masjid-masjid  sebagai tempat da’wah, data mubaligh,  aneka kegiatan muballigh di masjid-masjid dan informasi untuk muballigh, sehingga ummat dapat mengetahui siapa siapa muballigh yang akan bertugas menjadi khatib atau penceramah di masjidnya.
Multinomial Computation and Factorial Theorems for Artificial Intelligence and Cybersecurity
Authors: Annamalai Chinnaraji
Published: August 2022

Combinatorial techniques with factorial and multinomial computation are used as algorithms for the programs development to apply in artificial intelligence and cybersecurity. Methodological advances in combinatorics and mathematics play a vital role in machine learning and cryptology for data analysis and artificial intelligence-based cybersecurity for protection of the computing systems, devices, networks, programs and data from cyber-attacks. In connection with these ideas, this article is prepared for applications in computing science and cybersecurity. This paper presents computing and combinatorial formulae such as theorems on factorials, binomial coefficients, multinomial computation and probability and binomial distributions.
Botanical composition and quality of pasture forage using different-age grass stands
Authors: Privalova Kira
Published: August 2022

The results of studies on the quality of pasture forage when using grass stands 1–5, 1–13, 7–19 and 13–25 years of age are presented. The possibility of obtaining high-quality feed with a rational mode of use and an optimal level of fertilizer with a 25-year period of use has been experimentally substantiated.
Influence of the genotype on the productive indicators of Yaroslavl cows
Authors: Zyryanova Svetlana
Published: August 2022

The evaluation of the milk productivity of first-calf cows of different genotypes was carried out. The most optimal phenotypic dependences of the trait with milk yield in each group of animals, depending on blood type, have been established. Phenotypic correlations of signs of milk productivity of cows-daughters and their mothers for 305 days of the first lactation are presented.
Study and evaluation of breeding material awnless brome (Bromopsis inermis Leyss.) of lawn-pasture type in steppe conditions Central Chernozem region
Authors: Zolotarev Vladimir , Ivanov Iosif , Ostrikova Marina
Published: August 2022

Among the perennial bluegrass grasses, the awnless brome is the most popular and widespread crop in the herbage of Russia. All varieties of awnless brome egistered in the State Register are of the hay-pasture type of use. A new source material of a awnless brome lawn-pasture eco-type seedling with high decorative properties has been created.
Productivity and quality of perennial grasses depending on cultivation technologies
Authors: Sabirova Tatyana , LOBANOVA Anastasiya , Tihonov Aleksandr
Published: August 2022

The quality of the green mass of perennial grasses is considered according to the main agronomic indicators from the first to the third year of use. Three cuttings were carried out during the growing season. The material covers three cultivation technologies that differ in the composition of fertilizers acting on the crop. Extensive technology, taken as control, is designed to produce a crop in conditions of natural soil fertility. Biologized and high-intensity technologies provided for additional aftereffect of manure and the application of mineral fertilizers. The application rates of mineral fertilizers were P30K45 in the biologized technology and P90K135 in the high-intensity one. The study showed a significant difference in the ratio of the main components of the grass mixture. In the second cut, the legume component prevailed over the cereal component. Grasses prevailed in the first and third cuts. With all cultivation technologies, the grasses of the first year of use provided the highest yield. Chemical analysis of grass green mass samples showed that the highest collection of dry matter, metabolic energy, feed units and crude protein was obtained in the variants with the maximum dose of mineral fertilizers. In herbs of the third year of use, the highest collection was noted in the biologized technology.
The microbial production of kynurenic acid using Yarrowia lipolytica yeast growing on crude glycerol and soybean molasses
Authors: Rakicka-Pustułka Magdalena , Ziuzia Patrycja , Pierwoła Jan , Szymański Kacper , Wróbel-Kwiatkowska Magdalena , Lazar Zbigniew
Published: August 2022

Yarrowia lipolytica yeast are able to produce kynurenic acid—a very valuable compound acting as a neuroprotective and antioxidant agent in humans. The recent data proved the existence of the kynurenine biosynthesis pathway in this yeast cells. Due to this fact, the aim of this work was to enhance kynurenic acid production using crude glycerol and soybean molasses as cheap and renewable carbon and nitrogen sources. The obtained results showed that Y. lipolytica GUT1 mutants are able to produce kynurenic acid in higher concentrations (from 4.5 mg dm−3 to 14.1 mg dm−3) than the parental strain (3.6 mg dm−3) in the supernatant in a medium with crude glycerol. Moreover, the addition of soybean molasses increased kynurenic acid production by using wild type and transformant strains. The A-101.1.31 GUT1/1 mutant strain produced 17.7 mg dm−3 of kynurenic acid in the supernatant during 150 h of the process and 576.7 mg kg−1 of kynurenic acid in dry yeast biomass. The presented work proves the great potential of microbial kynurenic acid production using waste feedstock. Yeast biomass obtained in this work is rich in protein, with a low content of lipid, and can be a healthy ingredient of animal and human diet.
Development of a high specificity typing method for the detection of herpes simplex virus
Authors: Chen Zhu , Zhao Kaixuan , Tan Boyu , Tong Zengrui , He Ziyu , Luo Xiaofang , Cai Lei , Wang Hanming , Leung Polly H. M. , Chow Franklin Wang-Ngai , Chen Hui , Deng Yan
Published: August 2022

Herpes disease is caused by Herpes simplex virus (HSV). It has become one of the global health problems. This paper reports a method for HSV type testing. First specific primers sequence for HSV-1 and HSV-2 were selected, designed, and synthesized. Then, these amplification products were proved by sequencing and analysis. Lastly, we optimized the reaction system and PCR reaction program by orthogonal design and sensitivity testing. Results showed that the lowest concentration in HSV-type testing is about 6.67 × 106 copies/ml. Moreover, the specificity of detection was very high. So, this method has very great potentials for HSV type testing in clinical practice.
Progress of selective catalytic reduction denitrification catalysts at wide temperature in carbon neutralization
Authors: Lin Dehai , Zhang Longhui , Liu Zilin , Wang Baodong , Han Yifan
Published: August 2022

With the looming goal of carbon neutrality and increasingly stringent environmental protection policies, gas purification in coal-fired power plants is becoming more and more intense. To achieve the NOx emission standard when coal-fired power plants are operating at full load, wide-temperature denitrification catalysts that can operate for a long time in the range of 260–420°C are worthy of study. This review focuses on the research progress and deactivation mechanism of selective catalytic reduction (SCR) denitration catalysts applied to a wide temperature range. With the increasing application of SCR catalysts, it also means that a large amount of spent catalysts is generated every year due to deactivation. Therefore, it is necessary to recycle the wide temperature SCR denitration catalyst. The challenges faced by wide-temperature SCR denitration catalysts are summarized by comparing their regeneration processes. Finally, its future development is prospected.
Solventing out crystallization-basic magnesium carbonate percipitation for thorough phosphorus removal from ammonium tungstate solution
Authors: Yang Liang , Qu Jie , Gong Dandan , Wang Zhongbing , Wang Ruixiang , Wan Linsheng
Published: August 2022

The ammonium tungstate solution obtained by leaching scheelite with phosphate contains a large amount of phosphorus. For production of qualified ammonium paratungstate products, phosphorus must be deeply removed from the ammonium tungstate solution. In this study, a novel process for ammonium phosphate recovery and deep phosphorus removal from the solution was proposed. First, ammonium phosphate was crystallized and separated from the ammonium tungstate solution by blowing ammonia and cooling. Results showed that the crystallization ratio of phosphorus was above 95% under the conditions of an ammonia concentration of 4.18 mol/L, an initial phosphorus concentration ranging from 15 g/L to 30 g/L, a holding time of 60 min and the temperature of 20°C. Then, the small portion of phosphorus remaining in the ammonium tungstate solution was further deeply removed by basic magnesium carbonate percipitation. The phosphorus removal efficiency was above 99% and tungsten loss was less than 0.22% under the following conditions: the basic magnesium carbonate stoichiometric ratio was 1.5, the initial phosphorus concentration was ranging from 0.5 to 4 g/L, the reaction time was 120 min and temperature was 25°C. After phosphorus removal, the concentration of phosphorus in the ammonium tungstate solution was below 10 ppm, which meant deep phosphorus removal was achieved.
Machine learning for maternal health: Predicting delivery location in a community health worker program in Zanzibar
Authors: Fredriksson Alma , Fulcher Isabel R. , Russell Allyson L. , Li Tracey , Tsai Yi-Ting , Seif Samira S. , Mpembeni Rose N. , Hedt-Gauthier Bethany
Published: August 2022

Background

Maternal and neonatal health outcomes in low- and middle-income countries (LMICs) have improved over the last two decades. However, many pregnant women still deliver at home, which increases the health risks for both the mother and the child. Community health worker programs have been broadly employed in LMICs to connect women to antenatal care and delivery locations. More recently, employment of digital tools in maternal health programs have resulted in better care delivery and served as a routine mode of data collection. Despite the availability of rich, patient-level data within these digital tools, there has been limited utilization of this type of data to inform program delivery in LMICs.

Methods

We use program data from 38,787 women enrolled in Safer Deliveries, a community health worker program in Zanzibar, to build a generalizable prediction model that accurately predicts whether a newly enrolled pregnant woman will deliver in a health facility. We use information collected during the enrollment visit, including demographic data, health characteristics and current pregnancy information. We apply four machine learning methods: logistic regression, LASSO regularized logistic regression, random forest and an artificial neural network; and three sampling techniques to address the imbalanced data: undersampling of facility deliveries, oversampling of home deliveries and addition of synthetic home deliveries using SMOTE.

Results

Our models correctly predicted the delivery location for 68%–77% of the women in the test set, with slightly higher accuracy when predicting facility delivery versus home delivery. A random forest model with a balanced training set created using undersampling of existing facility deliveries accurately identified 74.4% of women delivering at home.

Conclusions

This model can provide a “real-time” prediction of the delivery location for new maternal health program enrollees and may enable early provision of extra support for individuals at risk of not delivering in a health facility, which has potential to improve health outcomes for both mothers and their newborns. The framework presented here is applicable in other contexts and the selection of input features can easily be adapted to match data availability and other outcomes, both within and beyond maternal health.
Pathfinder elements for gold exploration in the Hongqigou tectonic zone of the Wulonggou goldfield (Qinghai Province, Western China)
Authors: Zhen Shikun , Sun Meiqing , Xia Chulin , Zhang Wenzhao , Quan Chang’en , Han Zhihong , Du Yu
Published: August 2022

Gold is strongly enriched (up to 12 ppb) in the Hongqigou tectonic zone, which represents a potential metal source for the Wulonggou goldfield. In this study, a suite of 20 elements (Au, Ag, As, B, Ba, Bi, Cd, Co, Cu, Hg, Mn, Mo, Ni, Pb, Sb, Sn, Ti, V, W, and Zn) were analyzed on 30 altered rock samples from the tectonic zone. Compared with the average abundance of the earth's crust, these samples are generally enriched in Hg, have comparable Au, Ba, and As, and depleted in Ba, Co, and W. We suggested that Au, As, Hg, and Ba are the major ore-forming elements. The coupling relationship in multielement variation patterns between the altered rocks (in the tectonic zone) and wall rocks indicates wallrock involvement during the alteration process. Correlation statistical analysis (with SPSS software) on the 20 elements suggests that the elements of Ag, Cu, Pb, Hg, and Cd are closely related, and useful pathfinder elements include As, Sb, Hg, W, Sn, Cu, Ni, Mn, and Zn.
Methodological framework for the axiological paradigm in the learning environment
Authors: Dauyenov Yernur , Zhumataeva Engilika , Orynbekova Ainur
Published: August 2022

Modern conditions of education require the development of new methodological approaches for the implementation of the educational process. The purpose of study is to analyze the axiological paradigm in the Kazakhstan’s system of teacher training and determines experimentally the phases of its development in Kazakhstan in early 21st century. A total of 284 students participated in the experiment. The findings suggest that students in the control group repeated what was said during the lecture. Generally, they did not go beyond repetitions. Out of 142 students who were involved in the first phase of the experimental group, 80 students responded at the heuristic level, 12 students responded at the innovative level, and 8 students responded at the creative level. Students in the experimental group were capable of independent thinking beyond lectures. 8 students proved to be 40% capable of drawing conclusion to an unusual, phenomenally high degree. Practical implications and further research might focus on the special aspects of developing the axiological paradigm not only for students in Kazakhstan, but also in other countries in a comparative aspect. New research can also rely on the experiment’s methodological framework.
Lysine Acetyltransferases and Their Role in AR Signaling and Prostate Cancer
Authors: Jaiswal Bharti , Agarwal Akanksha , Gupta Ashish
Published: August 2022

The development and growth of a normal prostate gland, as well as its physiological functions, are regulated by the actions of androgens through androgen receptor (AR) signaling which drives multiple cellular processes including transcription, cellular proliferation, and apoptosis in prostate cells. Post-translational regulation of AR plays a vital role in directing its cellular activities via modulating its stability, nuclear localization, and transcriptional activity. Among various post-translational modifications (PTMs), acetylation is an essential PTM recognized in AR and is governed by the regulated actions of acetyltransferases and deacetyltransferases. Acetylation of AR has been identified as a critical step for its activation and depending on the site of acetylation, the intracellular dynamics and activity of the AR can be modulated. Various acetyltransferases such as CBP, p300, PCAF, TIP60, and ARD1 that are known to acetylate AR, may directly coactivate the AR transcriptional function or help to recruit additional coactivators to functionally regulate the transcriptional activity of the AR. Aberrant expression of acetyltransferases and their deregulated activities have been found to interfere with AR signaling and play a key role in development and progression of prostatic diseases, including prostate cancer (PCa). In this review, we summarized recent research advances aimed at understanding the role of various lysine acetyltransferases (KATs) in the regulation of AR activity at the level of post-translational modifications in normal prostate physiology, as well as in development and progression of PCa. Considering the critical importance of KATs in modulating AR activity in physiological and patho-physiological context, we further discussed the potential of targeting these enzymes as a therapeutic option to treat AR-related pathology in combination with hormonal therapy.
Effect of metformin versus metformin plus liraglutide on gonadal and metabolic profiles in overweight patients with polycystic ovary syndrome
Authors: Xing Chuan , Zhao Han , Zhang Jiaqi , He Bing
Published: August 2022

Objective

To observe the effect of metformin (MET) monotherapy versus MET plus liraglutide (LIRA) on gonadal and metabolic profiles in overweight patients with polycystic ovary syndrome (PCOS).

Methods

Sixty overweight patients with PCOS were recruited from January 2021 to January 2022 in Shengjing Hospital of China Medical University and were randomly assigned to the MET or combination (COM) group to receive 12 weeks of MET monotherapy or MET plus LIRA therapy. Anthropometric measurements, menstrual cycle changes, gonadal profiles, and oral glucose tolerance tests (OGTT) were conducted at baseline and after the 12-week treatment.

Results

Fifty-two subjects completed the trial while eight were lost during the follow-up. Both MET and COM improved menstrual cycles, anthropometric parameters, and glucose metabolism after the 12-week treatment; however, there was no statistical difference between the two groups. MET plus LIRA therapy improved hyperandrogenemia, including TT (total testosterone), SHBG (sex hormone binding globulin) and FAI (free androgen index), whereas MET monotherapy only improved SHBG and FAI when compared with baseline. Furthermore, both MET monotherapy and MET plus LIRA therapy improved E2 (estradiol) while only MET plus LIRA therapy improved LH (luteinizing hormone), FSH (follicle stimulating hormone) and Prog (progesterone) more effectively than baseline. Additionally, MET plus LIRA therapy may improve TT, SHBG, FAI, LH and Prog more effectively than MET monotherapy; however, there were no significant differences on E2, FSH and LH/FSH between the two groups.

Conclusions

In overweight patients with PCOS, both MET monotherapy and MET plus LIRA therapy improved glucose metabolism and relieved insulin resistance (IR). Additionally, MET plus LIRA therapy was more effective than MET monotherapy in improving reproductive abnormalities and hyperandrogenemia, potentially by modulating the hypothalamic-pituitary-ovarian axis.
Light intensity optimization of optical fiber stress sensor based on SSA-LSTM model
Authors: Yu Dakuan , Qiao Xueguang , Wang Xiangyu
Published: August 2022

In order to further improve the measurement range and accuracy of optical fiber stress sensor based on the interference between rising vortex beam and plane wave beam, a new stress demodulation model is designed. This model proposes a method to optimize the long-term and short-term memory network (LSTM) model by using sparrow search algorithm (SSA), extract the main characteristics of the influence of various variables on optical fiber stress sensor, and fit the relationship between sensor stress and beam phase difference. This method is an attempt of the deep learning model LSTM in the study of stress mediation model. There are very few related studies, and it is very necessary to fill this gap. In the experiment, the SSA-LSTM neural network is trained by using the data of stress and phase difference measured by the optical fiber stress sensor. The test results show that the mean error of SSA-LSTM neural network is less than that of LSTM neural network, which shows that the combination of SSA-LSTM model and optical fiber stress sensor can make its measurement accuracy higher, The algorithm can more effectively reduce the influence of the surrounding environment and the influence of the light source fluctuation on the measurement range and accuracy of the optical fiber sensor, and has good practical application value. It is proved that the deep learning LSTM neural network has good application value in the light intensity optimization of optical fiber stress sensor.
Genomic insight into the population history and biological adaptations of high-altitude Tibetan highlanders in Nagqu
Authors: Ma Lifeng , Wang Rui , Feng Siwei , Yang Xiaomin , Li Jing , Zhang Zhiying , Zhan Haoshuang , Wang Yao , Xia Ziyang , Wang Chuan-Chao , Kang Longli
Published: August 2022

Tibetan, one of the largest indigenous populations living in the high-altitude region of the Tibetan Plateau (TP), has developed a suite of physiological adaptation strategies to cope with the extreme highland environment in TP. Here, we reported genome-wide SNP data from 48 Kham-speaking Nagqu Tibetans and analyzed it with published data from 1,067 individuals in 167 modern and ancient populations to characterize the detailed Tibetan subgroup history and population substructure. Overall, the patterns of allele sharing and haplotype sharing suggested (1) the relatively genetic homogeny between the studied Nagqu Tibetans and ancient Nepalese as well as present-day core Tibetans from Lhasa, Nagqu, and Shigatse; and (2) the close relationship between our studied Kham-speaking Nagqu Tibetans and Kham-speaking Chamdo Tibetans. The fitted qpAdm models showed that the studied Nagqu Tibetans could be fitted as having the main ancestry from late Neolithic upper Yellow River millet farmers and deeply diverged lineages from Southern East Asians (represented by Upper Paleolithic Guangxi_Longlin and Laos_Hoabinhian), and a non-neglectable western Steppe herder-related ancestry (∼3%). We further scanned the candidate genomic regions of natural selection for our newly generated Nagqu Tibetans and the published core Tibetans via FST, iHS, and XP-EHH tests. The genes overlapping with these regions were associated with essential human biological functions such as immune response, enzyme activity, signal transduction, skin development, and energy metabolism. Together, our results shed light on the admixture and evolutionary history of Nagqu Tibetan populations.
DNA methylation affects freezing tolerance in winter rapeseed by mediating the expression of genes related to JA and CK pathways
Authors: Wei Jiaping , Shen Yingzi , Dong Xiaoyun , Zhu Yajing , Cui Junmei , Li Hui , Zheng Guoqiang , Tian Haiyan , Wang Ying , Liu Zigang
Published: August 2022

Winter rapeseed is the largest source of edible oil in China and is especially sensitive to low temperature, which causes tremendous agricultural yield reduction and economic losses. It is still unclear how DNA methylation regulates the formation of freezing tolerance in winter rapeseed under freezing stress. Therefore, in this study, the whole-genome DNA methylation map and transcriptome expression profiles of freezing-resistant cultivar NTS57 (NS) under freezing stress were obtained. The genome-wide methylation assay exhibited lower levels of methylation in gene-rich regions. DNA methylation was identified in three genomic sequence contexts including CG, CHG and CHH, of which CG contexts exhibited the highest methylation levels (66.8%), followed by CHG (28.6%) and CHH (9.5%). Higher levels of the methylation were found in upstream 2 k and downstream 2 k of gene regions, whereas lowest levels were in the gene body regions. In addition, 331, 437, and 1720 unique differentially methylated genes (DMGs) were identified in three genomic sequence contexts in 17NS under freezing stress compared to the control. Function enrichment analysis suggested that most of enriched DMGs were involved in plant hormones signal transduction, phenylpropanoid biosynthesis and protein processing pathways. Changes of genes expression in signal transduction pathways for cytokinin (CK) and jasmonic acid (JA) implied their involvement in freezing stress responses. Collectively, these results suggested a critical role of DNA methylation in their transcriptional regulation in winter rapeseed under freezing stress.
Single-cell RNA-Seq reveals the potential risk of anti-mesothelin CAR T Cell therapy toxicity to different organs in humans
Authors: Wen Lu , Huang Yu , Peng Ling , Zhao Kaiping , Sun Yan , Lin Zhicai , Chen Yuanyuan , Li Zhong , Qian Qijun , Tong Fan , Zhang Ruiguang , Dong Xiaorong
Published: August 2022

“On-target off-tumor” toxicity is a major challenge to the use of chimeric antigen receptor (CAR)-engineered T cells in the treatment of solid malignancies, because of the expression of target antigens in normal tissues. Mesothelin overexpression is associated with poor prognosis of multiple solid tumors, and would therefore appear to be a suitable antigen target. To understand the risk of toxicity to different organs on anti-mesothelin CAR T cell therapy, single-cell RNA sequencing (scRNA-seq) datasets derived from major human physiological systems were analyzed in this study, including the respiratory, cardiovascular, digestive, and urinary systems. According to scRNA-seq datasets, the organs were stratified into high or low risk based on the level of mesothelin expression. We report that the proportion of mesothelin-positive cells was 7.71%, 2.40% and 2.20% of myocardial cells, pulmonary cells and stomach cells, respectively, indicating that these organs could be at high risk of “on-target off-tumor” toxicity on anti-mesothelin CAR T cell therapy. By contrast, esophagus, ileum, liver, kidney and bladder exhibited low mesothelin expression (<1%). Therefore, these organs could be regarded as at low risk. Thus, the risk of toxicity to different organs and tissues in anti-mesothelin CAR T cell therapy may be predicted by these scRNA-seq data.
Heterologous immunization with adenovirus vectored and inactivated vaccines effectively protects against SARS-CoV-2 variants in mice and macaques
Authors: He Qian , Mao Qunying , Zhang Jialu , Gao Fan , Bai Yu , Cui Bopei , Liu Jianyang , An Chaoqiang , Wang Qian , Yan Xujia , Yang Jinghuan , Song Lifang , Song Ziyang , Liu Dong , Yuan Yadi , Sun Jing , Zhao Jincun , Bian Lianlian , Wu Xing , Huang Weijin , Li Changgui , Wang Junzhi , Liang Zhenglun , Xu Miao
Published: August 2022

To cope with the decline in COVID-19 vaccine-induced immunity caused by emerging SARS-CoV-2 variants, a heterologous immunization regimen using chimpanzee adenovirus vectored vaccine expressing SARS-CoV-2 spike (ChAd-S) and an inactivated vaccine (IV) was tested in mice and non-human primates (NHPs). Heterologous regimen successfully enhanced or at least maintained antibody and T cell responses and effectively protected against SARS-CoV-2 variants in mice and NHPs. An additional heterologous booster in mice further improved and prolonged the spike-specific antibody response and conferred effective neutralizing activity against the Omicron variant. Interestingly, priming with ChAd-S and boosting with IV reduced the lung injury risk caused by T cell over activation in NHPs compared to homologous ChAd-S regimen, meanwhile maintained the flexibility of antibody regulation system to react to virus invasion by upregulating or preserving antibody levels. This study demonstrated the satisfactory compatibility of ChAd-S and IV in prime-boost vaccination in animal models.
PIRCHE-II scores prove useful as a predictive biomarker among kidney transplant recipients with rejection: An analysis of indication and follow-up biopsies
Authors: Spitznagel Tahm , Matter Laurenz S. , Kaufmann Yves L. , Nilsson Jakob , von Moos Seraina , Schachtner Thomas
Published: August 2022

Background

Indication biopsies for deterioration of kidney allograft function often require follow-up biopsies to assess treatment response or lack of improvement. Immune-mediated injury, namely borderline rejection (BLR), T-cell mediated rejection (TCMR), or antibody-mediated rejection (ABMR), results from preformed or de novo alloreactivity due to donor and recipient HLA-mismatches. The impact of HLA-mismatches on alloreactivity is determined by highly immunogenic HLA-epitopes.

Methods

We analyzed 123 kidney transplant recipients (KTRs) from 2009 to 2019 who underwent a first indication and a follow-up biopsy. KTRs were divided into three groups according to the first biopsy: No rejection (NR)/BLR (n=68); TCMR (n=21); ABMR (n=34). The HLA-derived epitope-mismatches were calculated using the Predicted Indirectly Recognizable HLA-Epitopes (PIRCHE-II) algorithm.

Results

Group NR/BLR: KTRs with higher total PIRCHE-II scores were more likely to develop TCMR in the follow-up biopsy (p=0.031). Interestingly, these differences were significant for both HLA-class I- (p=0.017) and HLA-class II-derived (p=0.017) PIRCHE-II scores. Group TCMR: KTRs with ongoing TCMR in the follow-up biopsy were more likely to show higher total PIRCHE-II scores (median 101.50 vs. 74.00). Group ABMR: KTRs with higher total PIRCHE-II scores were more likely to show an increase in the microvascular inflammation score in the follow-up biopsy. This difference was more pronounced for the HLA-class II-derived PIRCHE-II scores (median 70.00 vs. 31.76; p=0.086).

Conclusions

PIRCHE-II scores may prove useful as a biomarker to predict the histopathological changes of immune-related injury from a first indication to a follow-up biopsy. This immunological risk stratification may contribute to individualized treatment strategies.
Mucosal immunity: The missing link in comprehending SARS-CoV-2 infection and transmission
Authors: Russell Michael W. , Mestecky Jiri
Published: August 2022

SARS-CoV-2 is primarily an airborne infection of the upper respiratory tract, which on reaching the lungs causes the severe acute respiratory disease, COVID-19. Its first contact with the immune system, likely through the nasal passages and Waldeyer’s ring of tonsils and adenoids, induces mucosal immune responses revealed by the production of secretory IgA (SIgA) antibodies in saliva, nasal fluid, tears, and other secretions within 4 days of infection. Evidence is accumulating that these responses might limit the virus to the upper respiratory tract resulting in asymptomatic infection or only mild disease. The injectable systemic vaccines that have been successfully developed to prevent serious disease and its consequences do not induce antibodies in mucosal secretions of naïve subjects, but they may recall SIgA antibody responses in secretions of previously infected subjects, thereby helping to explain enhanced resistance to repeated (breakthrough) infection. While many intranasally administered COVID vaccines have been found to induce potentially protective immune responses in experimental animals such as mice, few have demonstrated similar success in humans. Intranasal vaccines should have advantage over injectable vaccines in inducing SIgA antibodies in upper respiratory and oral secretions that would not only prevent initial acquisition of the virus, but also suppress community spreadviaaerosols and droplets generated from these secretions.
Neoadjuvant immunotherapy and chemoimmunotherapy for stage II-III muscle invasive bladder cancer
Authors: Chen Hualin , Yang Wenjie , Xue Xiaoqiang , Li Yingjie , Jin Zhaoheng , Ji Zhigang
Published: August 2022

Objective

Considering the striking evidence revealed by immunotherapy in advanced or metastatic bladder cancer, investigators have explored neoadjuvant immunotherapy and chemoimmunotherapy in muscle-invasive bladder cancer (MIBC). Currently, there have been a large number of studies reporting varied efficacy and safety of these approaches. Herein, we pooled the available evidence in terms of oncological outcomes (pathological complete response [pCR] and pathological partial response [pPR]) and safety outcomes (immune-related adverse events [irAEs], treatment-related adverse events [TRAEs]), through a systematic review and meta-analysis.

Method

We searched PubMed, Embase, Cochrane Library, and American Society of Clinical Oncology meeting abstracts to identify relevant studies up to June 2022. Studies were included if they evaluated the neoadjuvant immunotherapy or chemoimmunotherapy in MIBC and reported at least the pCR.

Results

A total of 22 records involving 843 patients were included. For pCR of immunotherapy, the pooled rate of immune checkpoint inhibitor (ICI) monotherapy and dual-ICIs therapy was 24% (95% confidence interval [CI]: 15.3% - 32.8%) and 32.1% (95%CI: 20.6% - 43.7%), respectively. For pCR of chemoimmunotherapy, the overall pooled rate was 42.6% (95% CI: 34.9% - 50.2%). Subgroup of gemcitabine/cisplatin (GC) plus ICI had a pCR rate of 41.7% (95%CI: 35.8% - 47.5%). In terms of safety, the pooled rate of Grade≥3 irAEs was 11.7% (95% CI: 6.5%-16.9%). In subgroup analysis, the Grade≥3 irAEs rate of ICI monotherapy, dual-ICIs therapy, and GC plus ICI therapy was 7.4% (95% CI: 4.3%-10.5%), 30.3% (95% CI: 15.3%-45.3%), and 14.5% (95% CI: 3.5% - 25.4%), respectively. Besides, the pooled Grade≥3 TRAEs rate for chemoimmunotherapy was 32.4% (95% CI: 13.1% - 51.6%).

Conclusion

Neoadjuvant immunotherapy and chemoimmunotherapy were effective and safe in the treatment of MIBC. Compared to ICI monotherapy, dual-ICIs therapy or chemoimmunotherapy can improve the response rate, while increasing the morbidity of Grade≥ 3 irAEs or Grade≥ 3 TRAEs.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier CRD4202233771.