bluuebunny/crossref_metadata_2025 huggingface dataset:
WO3/BiOBr S-Scheme Heterojunction Photocatalyst for Enhanced Photocatalytic CO2 Reduction
Authors: Li Chen , Lu Xingyu , Chen Liuyun , Xie Xinling , Qin Zuzeng , Ji Hongbing , Su Tongming
Published: July 2024

The photocatalytic CO2 reduction strategy driven by visible light is a practical way to solve the energy crisis. However, limited by the fast recombination of photogenerated electrons and holes in photocatalysts, photocatalytic efficiency is still low. Herein, a WO3/BiOBr S-scheme heterojunction was formed by combining WO3 with BiOBr, which facilitated the transfer and separation of photoinduced electrons and holes and enhanced the photocatalytic CO2 reaction. The optimized WO3/BiOBr heterostructures exhibited best activity for photocatalytic CO2 reduction without any sacrificial reagents, and the CO yield reached 17.14 μmol g−1 after reaction for 4 h, which was 1.56 times greater than that of BiOBr. The photocatalytic stability of WO3/BiOBr was also improved.
Influence of Silica Modulus on the Activation of Amorphous Wollastonitic Hydraulic Binders with Different Alumina Content: Study of Hydration Reaction and Paste Performance
Authors: Antunes Mónica , Santos Rodrigo Lino , Horta Ricardo Bayão , Colaço Rogério
Published: July 2024

This study investigates how different sodium silicate SiO2/Na2O MS ratios (0.75, 0.9, and 1.2) affect the hydration behavior of amorphous wollastonitic hydraulic (AWH) binders containing various amounts of Al2O3 content (4, 7, 10, and 12%wt). The effects of and interaction between the MS ratio of the activator and the Al2O3 content of the sample on the hydration reaction and paste performance were investigated. The reaction was followed by calorimetry, and the pastes’ compressive strength performances were tested at different curing times (2, 7, and 28 days). The hydrated pastes were characterized by FTIR, thermogravimetry analysis, and X-ray diffraction. The calorimetric results show that a higher Al2O3 cContent and a higher MS ratio result in a longer induction period. In terms of paste performance, an increase of the Al2O3 coupled with an activation with a 1.2 MS ratio results in a lower compressive strength after 28 days of hydration; the results range from 76 to 52 MPa. A decrease of the MS ratio to 0.9 allowed the obtention of a narrower range of results, from 76 to 69 MPa. Even though a decrease of the MS ratio to 0.75 led to higher hydration kinetics and high compressive strength results at early ages, at 28 days of curing, a decrease in compressive strength was observed. This may be a consequence of the fast kinetic of the mixture, since the rapid growth of hydration products may inhibit the dissolution at later ages and increase the porosity of the paste. Moreover, the high Al intake in the hydration product, facilitated by the high sodium content of the activator, promotes the formation of a higher number of calcium aluminate silicate hydrate structures (C-A-S-H) to the detriment of calcium silicate hydrate structures (C-S-H), decreasing the compressive strength of the samples. The TGA results indicate that the samples hydrated with the MS075 solution resulted in a higher number of hydrated products at early ages, while the samples hydrated with the MS09 and MS1.2 solutions exhibit a steady increase with curing time. Hence, an equilibrium in the hydration kinetic promoted by Si saturation–undersaturation appears to be fundamental in this system, which is influenced by both the MS ratio and the Al(OH)4− content in solution. The results of this study suggest that for this type of binder, optimal performance can be achieved by decreasing the MS ratio to 0.9. This composition allows for a controlled kinetic and overall higher compressive strength results in pastes produced with this AWH precursor.
Micro- and Macroscopic Analysis of Fatigue Wear of Gear Wheel Top Layer—An Impact Analysis of Thermochemical Treatment
Authors: Osiński Piotr , Dudziński Włodzimierz , Deptuła Adam , Łuszczyna Rafał , Kalita Marek
Published: July 2024

Today, there are many diagnostic methods and advanced measurement techniques enabling the correct diagnosis and assessment of the type and degree of wear of cogwheels (gears, pumps, etc.). The present study presents an analysis of the surface defects of a cogwheel of an oil pump prototype (3PW-BPF-24). The test object operated for a certain number of hours under controlled operating and environmental parameters. The damage to the surface layer was caused by fatigue phenomena and previous thermo-chemical treatment. On the basis of the significant percentage share (~30%) of residual austenite in the volume of the diffusion layer, a hypothetical conclusion was drawn about the suboptimal parameters of the thermo-chemical treatment process (in relation to the chemical composition of the analyzed pinion). A large number of research studies indicate that the significant presence of residual austenite causes a decrease in tooth surface hardness, the initiation of brittle cracks, a sharp decrease in fatigue strength, an increase in brittleness and a tendency to develop surface layer cracks during operation. High-resolution 3D scans of randomly selected pitting defects were used in the detailed study of the present work. It was indicated that the analysis of the morphology of surface defects allowed some degree of verification of the quality of the heat/chemical treatment. The martensitic transformation of residual austenite under controlled (optimum) repeated heat treatment conditions could significantly improve the durability of the pinion (cogwheel). In the case analyzed, the preferred treatment was the low-temperature treatment. The paper concludes with detailed conclusions based on the microscopic and macroscopic investigations carried out.
Study on the Effects of Printing Process and Reinforcement Materials on the Performance of 3D-Printed Glass Bead Insulation Mortar
Authors: Wang Jun , Zhang Xuanzheng , Liu Zhenhua , Zhao Jiasheng
Published: July 2024

Based on 3D printing technology, this paper investigates the effects of the printing process and reinforcement materials on the performance of 3D-printed glass bead insulation mortar. In order to improve and enhance the performance of the mortar, two sets of tests were designed for research and analysis. Firstly, by changing the direction of the interlayer printing strips, the anisotropy of the specimens in different paths was analyzed, and then the effect of different dosages of different fibers on the performance of 3D-printed glass bead insulation mortar was investigated by adding reinforcing materials. The results show that the path a specimen in the X direction’s compressive strength is the best; in the Y direction, flexural strength is the best; the path b specimen in the Y direction’s compressive strength is the best; in the Z direction, flexural strength is the best, but the compressive and flexural strengths are lower than the strength of the specimen without 3D printing (cast-in-place specimen); and adding reinforcing materials mortar not only has high strength but also has good printability and excellent thermal insulation. This paper provides a theoretical basis and reference value for the popularization and application of 3D printing thermal insulation mortar technology.
Preparation of Sn-Doped Ga2O3 Thin Films and MSM Ultraviolet Detectors Using Magnetron Co-Sputtering
Authors: Liu Yantao , Huang Rong , Lin Tao , Dang Jiale , Huang Haoxiang , Shi Jiahao , Chen Sui
Published: July 2024

Sn-doped Ga2O3 thin films and metal–semiconductor–metal (MSM) ultraviolet detectors were prepared using the co-sputtering method to enhance their photoelectric performance. The results revealed that Sn doping can effectively change the optical and electrical properties of thin films, greatly improving the photoelectric responsiveness of the devices. Through microstructure testing results, all of the thin film structures were determined to be monoclinic beta phase gallium oxide. At a DC power of 30 W, the thickness of the Sn-doped thin film was 430 nm, the surface roughness of the thin film was 4.94 nm, and the carrier concentration, resistivity, and mobility reached 9.72 × 1018 cm−3, 1.60 × 10−4 Ω·cm, and 45.05 cm3/Vs, respectively. The optical results show that Sn doping clearly decreases the transmission of thin films and that the bandgap can decrease to 3.91 eV. Under 30 W DC power, the photo dark current ratio of the detector can reach 101, time responses of tr = 31 s and tf = 22.83 s were obtained, and the spectral responsivity reached 19.25 A/W.
High-Emissivity Double-Layer ZrB2-Modified Coating on Flexible Aluminum Silicate Fiber Fabric with Enhanced Oxidation Resistance and Tensile Strength
Authors: Li Wei , Zhang Xueying , Yan Liwen , Guo Anran , Du Haiyan , Liu Jiachen
Published: July 2024

Fibers crystallize and become brittle at high temperatures for a long time, so the surface coating must maintain long-lasting emission performance, which requires superior antioxidant properties of the high-emissivity fillers. To improve the radiation performance of the coating and the tensile strength of the fiber fabric, a double-layer coating with high emissivity was prepared on the surface of flexible aluminum silicate fiber fabric (ASFF) using MoSi2 and SiC as emissive agents. The incorporation of borosilicate glass into the outer coating during high-temperature oxidation of ZrB2 results in superior encapsulation of emitter particles, effectively filling the pores of the coating and significantly reducing the oxidation rate of MoSi2 and SiC. Furthermore, the addition of an intermediate ZrO2 layer enhances the fiber bundle’s toughness. The obtained double-coated ASFF exhibits an exceptionally high tensile strength of 57.6 MPa and a high bond strength of 156.2 kPa. After being subjected to a 3 h heating process, the emissivity exhibits a minimal decrease of only 0.032, while still maintaining a high value above 0.9. The thermal insulation composites, consisting of a flexible ASFF matrix and a ZrB2-modified double-layer coating, exhibit significant potential for broad applications in the field of thermal protection.
Enhancing Magnetic Performance of FeNi50 Soft Magnetic Composites with Double-Layer Insulating Coating for High-Frequency Applications
Authors: Zheng Weizhong , Zhou Zixin , Zou Rongyu , Yang Minghui
Published: July 2024

Soft magnetic composites (SMCs) such as FeNi50 are indispensable in modern electronics due to their high magnetic permeability and low-loss characteristics, meeting the requirements for miniaturization and high-frequency operation. However, the integration of organic materials, initially aimed at reducing the total losses, presents challenges by introducing thermal stability issues at high frequencies. To overcome this obstacle, we propose a double-layer insulating coating method, applying a complete inorganic/organic composite insulation layer to the surface of iron–nickel magnetic powder. The double-layer insulating coating insulation method aims to reduce the total losses, particularly the eddy-current losses prevalent in SMCs. Additionally, the double-layer insulating coating method helps alleviate the thermal stability issues associated with organic materials at high frequencies, ultimately enhancing the magnetic properties of SMCs. We systematically investigated the influence of different resin types on the microstructure of the double-layer insulating coating, accompanied by a comprehensive comparison of the magnetic properties of the resulting samples. The experimental findings demonstrate a significant reduction in the eddy-current losses through the double-layer insulating coating method, with the total losses decreasing by over 95% compared to the initial FeNi50 magnetic powder composite (MPC) materials. Notably, the sodium silicate and silicone resins exhibited superior performances as double-layer insulating coatings, achieving total loss reductions of 1350 W/kg and 1492 W/kg, respectively. In conclusion, the double-layer insulating coating method addresses the challenges related to the total losses and thermal stability in SMCs, offering a promising approach to improve their performance in various electrical and electronic applications.
Alternative View of Inextensible Flows of Curves and Ruled Surfaces via Alternative Frame
Authors: Savić Ana , Eren Kemal , Ersoy Soley , Baltić Vladimir
Published: July 2024

In this paper, we present the evolutions of ruled surfaces generated by the principal normal, the principal normal’s derivative, and the Darboux vector fields along a space curve that are the elements of an alternative frame. The comprehension of an object’s rotational behavior is crucial knowledge relevant to various realms, and this can be accomplished by analyzing the Darboux vector along the path of a point on the object as it moves through space. In that regard, examining the evolutions of the ruled surfaces based on the changes in their directrices, including the Darboux vector in the alternative frame along a space curve, is significant. As the first step of this study, we express the evolution of the alternative frame elements of a space curve. Subsequently, the conditions for the ruled surfaces generated by them to be minimal, developable, and inextensible are investigated. These findings can allow some physical phenomena to be well understood through surface evolutions satisfying these conditions. In the final step, we provide graphical representations of some examples of inextensible ruled surfaces and curve evolutions.
Generalized Linear Model (GLM) Applications for the Exponential Dispersion Model Generated by the Landau Distribution
Authors: Bar-Lev Shaul K. , Liu Xu , Ridder Ad , Xiang Ziyu
Published: July 2024

The exponential dispersion model (EDM) generated by the Landau distribution, denoted by EDM-EVF (exponential variance function), belongs to the Tweedie scale with power infinity. Its density function does not have an explicit form and, as of yet, has not been used for statistical aspects. Out of all EDMs belonging to the Tweedie scale, only two EDMs are steep and supported on the whole real line: the normal EDM with constant variance function and the EDM-EVF. All other absolutely continuous steep EDMs in the Tweedie scale are supported on the positive real line. This paper aims to accomplish an overall picture of all generalized linear model (GLM) applications belonging to the Tweedie scale by including the EDM-EVF. This paper introduces all GLM ingredients needed for its analysis, including the respective link function and total and scaled deviance. We study its analysis of deviance, derive the asymptotic properties of the maximum likelihood estimation (MLE) of the covariate parameters, and obtain the asymptotic distribution of deviance, using saddlepoint approximation. We provide numerical studies, which include estimation algorithm, simulation studies, and applications to three real datasets, and demonstrate that GLM using the EDM-EVF performs better than the linear model based on the normal EDM. An R package accompanies all of these.
Graph-Based Hotspot Detection of Socio-Economic Data Using Rough-Set
Authors: Tabarej Mohd Shamsh , Minz Sonajharia , Shaikh Anwar Ahamed , Shuaib Mohammed , Jeribi Fathe , Alam Shadab
Published: July 2024

The term hotspot refers to a location or an area where the occurrence of a particular phenomenon, event, or activity is significantly higher than in the surrounding areas. The existing statistical methods need help working well on discrete data. Also, it can identify a false hotspot. This paper proposes a novel graph-based hotspot detection using a rough set (GBHSDRS) for detecting the hotspots. This algorithm works well with discrete spatial vector data. Furthermore, it removes the false hotspot by finding the statistical significance of the identified hotspots. A rough set theory is applied to the graph of the spatial polygon data, and the nodes are divided into lower, boundary, and negative regions. Therefore, the candidate hotspot belongs to the lower region of the set, and the boundary value analysis will ensure the identification of the hotspots if the hotspot is present in the dataset. The p-value is used to find the statistical significance of the hotspots. The algorithm is tested on the socioeconomic data of Uttar Pradesh (UP) from 1991 on medical facilities. The average gain in density and Hotspot Prediction Accuracy Index (HAPI) of the detected hotspots is 26.54% and 23.41%, respectively. An average reduction in runtime is 27.73%, acquired compared to all other methods on the socioeconomic data.
Additive Results of Group Inverses in Banach Algebras
Authors: Liu Dayong , Chen Huanyin
Published: July 2024

In this paper, we present new presentations of group inverses for the sum of two group invertible elements in a Banach algebra. We then apply these results to block complex matrices. The group invertibility of certain block complex matrices is thereby obtained.
Optimal Reinsurance and Derivative-Based Investment Decisions for Insurers with Mean-Variance Preference
Authors: Zhou Haiying , Zhu Huainian
Published: July 2024

In our study, we investigate reinsurance issues and optimal investment related to derivatives trading for a mean-variance insurer, employing game theory. Our primary objective is to identify strategies that are time-consistent. In particular, the insurer has the flexibility to purchase insurance in proportion to its needs, explore new business, and engage in capital market investments. This is under the assumption that insurance companies’surplus capital adheres to the classical Cramér-Lundberg model. The capital market is made up of risk-free bonds, equities, and derivatives, with pricing dependent on the underlying stock’s basic price and volatility. To obtain the most profitable expressions and functions for the associated investment strategies and time guarantees, we solve a system of expanded Hamilton–Jacobi–Bellman equations. In addition, we delve into scenarios involving optimal investment and reinsurance issues with no derivatives trading. In the end, we present a few numerical instances to display our findings, demonstrating that the efficient frontier in the case of derivative trading surpasses that in scenarios where derivative trading is absent.
Deep Smooth Random Sampling and Association Attention for Air Quality Anomaly Detection
Authors: Wang Peng , Li Minhang , Zhi Xiaoying , Liu Xiliang , He Zhixiang , Di Ziyue , Zhu Xiang , Zhu Yanchen , Cui Wenqiong , Deng Wenyu , Fan Wenhan
Published: July 2024

Real-time monitoring and timely warning of air quality are vital components of building livable cities and implementing the “Healthy China” strategy. Real-time, efficient, and accurate detection of air quality anomalies holds great significance. However, almost all existing methods for air quality anomaly detection often overlook the imbalanced distribution of data. In addition, many traditional methods cannot learn both pointwise representation and pairwise association, so they cannot solve complex features. This study proposes an anomaly detection method for air quality monitoring based on Deep Smooth Random Sampling and Association Attention in Transformer (DSRS-AAT). Firstly, based on the third geographical law, the more similar the geographical environment, the closer the geographical target features are. We cluster sites according to the surrounding geographic features to fully explore latent feature associations. Then, we employ Deep Smooth Random Sampling to rebalance the air quality datasets. Meanwhile, the Transformer with association attention considers both prior associations and series associations to distinguish anomaly patterns. Experiments are carried out with real data from 95 monitoring stations in Haikou City, China. Final results demonstrate that the proposed DSRS-AAT improves the effectiveness of anomaly detection and provides interpretability analysis for traceability, owing to a significant improvement with the baselines (OmniAnomaly, THOC, etc.). The proposed method effectively enhances the effectiveness of air quality anomaly detection and provides a reference value for real-time monitoring and early warning of urban air quality.
Forecast Horizon of Dynamic Lot Sizing Problem with Perishable Inventory and Multiple Chain Stores: Shipping and Stockout Cost
Authors: Xue Feng , Li Qiumin
Published: July 2024

Perishable products are very common, but managing inventory of perishable products can be very challenging for firms, especially in distribution systems, including multiple chain stores. In this environment, we consider a dynamic lot-sizing problem faced by a distribution center that dis-patches a single perishable product to multiple chain stores. Demand cannot be backlogged, but it does not have to be satisfied; unsatisfied demand means stockout (lost sale). The first step is to transform the total profit function into a special total cost function. Our next step is to explore the properties of the optimal solution and use them to formulate a dynamic programming algorithm to solve the problem. Furthermore, we establish forecast and decision horizon results, which help the operation manager to decide the precise forecast horizon in a rolling decision-making process. Based on the model setting and the methods of dynamic programming, we obtained two interesting findings: (1) the maximized profit objective function is equivalent to the minimized cost objective function, and (2) the famous zero inventory property conditionally holds in the inventory management of perishable products. On an extensive test bed, useful insights were obtained on the impact of the lifetime of the product and cost parameters on the total cost and length of the forecast horizon. Thus, the contributions of this study are as follows: (1) we explore two structure policies in an optimal solution to devise efficient algorithms to reduce computational complexity; (2) we provide a sufficient condition for forecasting and decision horizons; and (3) we determine that, for a given fixed cost, the median forecast horizon first increases with the lifetime of the product and stockout cost and then remains invariable when it reaches a certain level.
Immunomodulatory Compounds from the Sea: From the Origins to a Modern Marine Pharmacopoeia
Authors: Cutolo Edoardo Andrea , Campitiello Rosanna , Caferri Roberto , Pagliuca Vittorio Flavio , Li Jian , Agathos Spiros Nicolas , Cutolo Maurizio
Published: July 2024

From sea shores to the abysses of the deep ocean, marine ecosystems have provided humanity with valuable medicinal resources. The use of marine organisms is discussed in ancient pharmacopoeias of different times and geographic regions and is still deeply rooted in traditional medicine. Thanks to present-day, large-scale bioprospecting and rigorous screening for bioactive metabolites, the ocean is coming back as an untapped resource of natural compounds with therapeutic potential. This renewed interest in marine drugs is propelled by a burgeoning research field investigating the molecular mechanisms by which newly identified compounds intervene in the pathophysiology of human diseases. Of great clinical relevance are molecules endowed with anti-inflammatory and immunomodulatory properties with emerging applications in the management of chronic inflammatory disorders, autoimmune diseases, and cancer. Here, we review the historical development of marine pharmacology in the Eastern and Western worlds and describe the status of marine drug discovery. Finally, we discuss the importance of conducting sustainable exploitation of marine resources through biotechnology.
ROS Induced by Aphrocallistes vastus Lectin Enhance Oncolytic Vaccinia Virus Replication and Induce Apoptosis in Hepatocellular Carcinoma Cells
Authors: Zhang Yanan , Zhu Ying , Jiang Gaohui , Chen Ke , Zhang Guohui , Chen Kan , Ye Ting , Zhou Yanrong , Li Gongchu
Published: July 2024

Oncolytic virotherapy is expected to provide a new treatment strategy for cancer. Aphrocallistes vastus lectin (AVL) is a Ca2+-dependent lectin receptor containing the conserved domain of C-type lectin and the hydrophobic N-terminal region, which can bind to the bird’s nest glycoprotein and D-galactose. Our previous studies suggested that the oncolytic vaccinia virus (oncoVV) armed with the AVL gene exerted remarkable replication and antitumor effects in vitro and in vivo. In this study, we found that oncoVV-AVL may reprogram the metabolism of hepatocellular carcinoma cells to promote ROS, and elevated ROS subsequently promoted viral replication and induced apoptosis. This study will provide a new theoretical basis for the application of oncoVV-AVL in liver cancer.
Hypervalent Iodine-Catalyzed Fluorination of Diene-Containing Compounds: A Computational Study
Authors: Liu Tianci , Li Hai-Bei
Published: July 2024

Studies have shown that the incorporation of fluorine into materials can improve their properties, but C–F bonds are not readily formed in nature. Although some researchers have studied the reaction of fluorinating alkenes catalyzed by hypervalent iodine, far too little attention has been paid to its reaction mechanism. This study aimed to explore the mechanism of the hypervalent iodine-catalyzed 1,4-difluorination of dienes. We found that the catalyst is favorable for the activation of C1=C2 double bonds through halogen bonds, and then two HFs interact with one F atom in the catalyst via hydrogen bonds, resulting in the cleavage of I–F bonds and the formation of [F–H∙∙∙F]−. Subsequently, the catalyst interacts with C1, and the roaming [F–H···F]− attacks C4 from the opposite side of the catalyst. After the fluorination step is completed, the nucleophile F− substitutes the catalyst via the SN2 mechanism. Our calculations demonstrated that the interaction between HF and F− is favorable for the stabilization of the transition state within the fluorination process for which the presence of two HFs in the reaction is the best. We also observed that [F–H∙∙∙F]− attacking C4 from the opposite side of the catalyst is more advantageous than attacking from the same side. This study therefore offers a novel perspective on the mechanism of the hypervalent iodine-catalyzed fluoridation of dienes.
Maximizing N-Nitrosamine Rejection via RO Membrane Plugging with Hexylamine and Hexamethylenediamine
Authors: Morović Silvia , Drmić Katarina Marija , Babić Sandra , Košutić Krešimir
Published: July 2024

The rapid expansion of urban areas and the increasing demand for water resources necessitate substantial investments in technologies that enable the reuse of municipal wastewater for various purposes. Nonetheless, numerous challenges remain, particularly regarding disinfection by-products (DBPs), especially carcinogenic compounds such as N-nitrosamines (NTRs). To tackle the ongoing issues associated with reverse osmosis (RO) membranes, this study investigated the rejection of NTRs across a range of commercially available RO membranes. In addition, the research aimed to improve rejection rates by integrating molecular plugs into the nanopores of the polyamide (PA) layer. Hexylamine (HEX) and hexamethylenediamine (HDMA), both linear chain amines, have proven to be effective as molecular plugs for enhancing the removal of NTRs. Given the environmental and human health concerns associated with linear amines, the study also aimed to assess the feasibility of diamine molecules as potential alternatives. The application of molecular plugs led to changes in pore size distribution (PSD) and effective pore number, resulting in a decrease in membrane permeability (from 5 to 33%), while maintaining levels suitable for RO processes. HEX and HDMA exhibited a positive effect on NTR rejection with ACM1, ACM5 and BW30LE membranes. In particular, NDMA rejection, the smallest molecule of the tested NTRs, with ACM1 was improved by 65.5% and 70.6% after treatment with HEX and HDMA, respectively.
Exploring Disulfiram’s Anticancer Potential: PLGA Nano-Carriers for Prolonged Drug Delivery and Potential Improved Therapeutic Efficacy
Authors: Dumbuya Ibrahim , Pereira Ana Maria , Tolaymat Ibrahim , Al Dalaty Adnan , Arafat Basel , Webster Matt , Pierscionek Barbara , Khoder Mouhamad , Najlah Mohammad
Published: July 2024

Disulfiram (DS) has been shown to have potent anti-cancer activity; however, it is also characterised by its low water solubility and rapid metabolism in vivo. Biodegradable polylactic-co-glycolic acid (PLGA) polymers have been frequently employed in the manufacturing of PLGA nano-carrier drug delivery systems. Thus, to develop DS-loaded PLGA nanoparticles (NPs) capable of overcoming DS’s limitations, two methodologies were used to formulate the NPs: direct nanoprecipitation (DNP) and single emulsion/solvent evaporation (SE), followed by particle size reduction. The DNP method was demonstrated to produce NPs of superior characteristics in terms of size (151.3 nm), PDI (0.083), charge (−37.9 mV), and loading efficiency (65.3%). Consequently, NPs consisting of PLGA and encapsulated DS coated with mPEG2k-PLGA at adjustable ratios were prepared using the DNP method. Formulations were then characterised, and their stability in horse serum was assessed. Results revealed the PEGylated DS-loaded PLGA nano-carriers to be more efficient; hence, in-vitro studies testing these formulations were subsequently performed using two distinct breast cancer cell lines, showing great potential to significantly enhance cancer therapy.
The Hydrothermal-Assisted Approach Improves the Photocatalytic and Energy Storage Performance of Novel CuSe-TiO2-GO Composite
Authors: Khan Afaq Ullah , Tahir Kamran , Shah Muhammad Zia Ullah , Albaqawi Hissah Saedoon , Almarhoon Zainab M. , Alanazi Abdulaziz A. , Alkudaisi Nora Awad , Althagafi Talal M. , Badi Nacer , Zaki Magdi E. A.
Published: July 2024

This study reports a novel CuSe-TiO2-GO composite, synthesized by a facile hydrothermal method at a controlled temperature, and investigates its electrochemical performance for supercapacitors (SCs) and photocatalytic behavior for degrading methylene blue (MB) dye. The compositional phase structure and chemical bond interaction were thoroughly investigated. The as-fabricated pristine, binary, and ternary composites underwent comprehensive characterization employing spectroscopic techniques and electrochemical analysis. Compared with pure and binary compounds (CuSe, TiO2, and binary CuSe-TiO2 composites), the ternary CuSe-TiO2-GO composites demonstrated a high degradation efficiency while degrading MB in less than just 80 min (240 min, 100 min, and 140 min, respectively). The photocatalytic activity of the ternary CuSe-TiO2-GO composites is enhanced due to the highly positive conduction band of CuSe, leading to the quick excitation of electrons to the conduction band of CuSe. Subsequently, graphene oxide (GO) left holes on the photocatalyst surface for MB, as GO assisted the photoexcited electron–hole pairs, resulting in enhanced photocatalytic performance. The CuSe-TiO2-GO electrode for the supercapacitor indicates a 310.6 F/g and 135.2 F/g capacitance when the discharge current upsurges from 1 to 12 A/g. The good photocatalytic and energy storage performance is due to the smaller charge transfer resistance, which promotes efficient separation of electron–hole pairs.
Sunflower Oil and Cholesterol Nanoemulsion: A Novel Carrier for Micafungin to Combat Multi-Resistant Candida auris
Authors: Marena Gabriel Davi , López Alejandro , Carvalho Gabriela Corrêa , Marín María del Pilar , Pérez Ruiz María Dolores , Pérez-Royo Jose Manuel , Tormo-Mas María Ángeles , Bernabé Patricia , Valentín Eulogio , Bauab Taís Maria , Chorilli Marlus , Pemán Javier , Ruiz-Gaitán Alba
Published: July 2024

Candida auris is an emerging, multidrug-resistant yeast that causes systemic infections, mainly in hospitalized or immunosuppressed patients. This pathogen has a high mortality and morbidity rate. This study aims to evaluate the antifungal potential of micafungin (MICA) encapsulated in a nanoemulsion (NEM) against four clades of C. auris and other non-C. auris species. The antifungal potential of MICA and NEM was evaluated by determining mature biofilm inhibition (0.78–50 µg/mL). The antifungal activities of MICA and NEM (5.92 mg/Kg) were evaluated using an in vivo model of Galleria mellonella. The results showed that NEM intensified the antibiofilm action of MICA, especially in 48 h mature biofilms. In vivo results displayed a higher effectiveness of NEM against all clades of C. auris tested, inhibiting the fungal load in the hemolymph and tissues of G. mellonella with a difference of 3 log10. In addition, C. auris infection caused granulomas surrounded by hemocytes, mainly at the lower and upper ends. Conversely, C. albicans developed pseudohyphae, biofilms, filaments, and chlamydospores. In conclusion, encapsulation of MICA in a nanoemulsion enhances its antifungal activity against mature biofilms of C. auris. This strategy may be considered a therapeutic approach for the control of infections and the dissemination of this new global health threat.
Genetic Characterization of Palyam Serogroup Viruses Isolated in Japan from 1984 to 2018 and Development of a Real-Time RT-PCR Assay for Broad Detection of Palyam Serogroup Viruses and Specific Detection of Chuzan (Kasba) and D’Aguilar Viruses
Authors: Shirafuji Hiroaki , Kishida Natsumi , Murota Katsunori , Suda Yuto , Yanase Tohru
Published: July 2024

We performed whole genome sequencing (WGS) of 15 Palyam serogroup virus (PALV) strains isolated from cattle or Culicoides biting midges in Japan from 1984 to 2018. We found that the PALV strains consisted of Chuzan (Kasba) virus (CHUV), D‘Aguilar virus (DAGV), Bunyip Creek virus, and another PALV, Marrakai virus (MARV). The Japanese MARV strains isolated in 1997 were closely related to Australian PALV strains isolated in 1968–1976 in genome segments 2 and 10, but they were most closely related to other Japanese PALV strains in the other genome segments. Our data suggest that the Japanese MARV strains were reassortant viruses between Asian and Australian PALVs. In addition to the WGS, we developed a real-time reverse-transcription polymerase chain reaction assay that can broadly detect PALV and specifically detect CHUV and DAGV, utilizing the data obtained by the WGS in this study. We detected the DAGV gene in bovine stillborn fetuses and congenitally abnormal calves in 2019 using the newly developed assay. To our knowledge, this is the first report of isolation of MARV outside of Australia and the first report of detection of PALV in bovine fetuses or calves with congenital abnormality outside of Africa.
Isolation, Identification, and Characterisation of a Novel ST2378 Aeromonas hydrophila Strain from Naturally Diseased Frogs, Rana dybowskii
Authors: Zhao Ran , Wang Jing , Wang Di , Wang Yanan , Hu Guo , Li Shaowu
Published: July 2024

In 2023, Rana dybowskii exhibiting characteristic skin ulcers were found on a farm in northeastern China. Subsequently, two dominant bacteria, Aeromonas hydrophila Rd001 and Acinetobacter johnsonii Rd002, were isolated from naturally infected R. dybowskii. Experimental infection confirmed that Rd001 was the primary pathogen responsible for the disease in R. dybowskii, with a mean lethal dose (LD50) of 6.25 × 102 CFU/g. The virulence genotype of Rd001 was identified as ser+/aha+/lip+/nuc+/hlyA+/aer+/alt+/ast+/act+. Antimicrobial susceptibility testing indicated that Rd001 was sensitive to enrofloxacin, flumequine, and neomycin. MLST analysis showed that Rd001 belonged to a new sequence type of A. hydrophila, named ST2378. This study offered the first comprehensive investigation into the pathogenicity, virulence genotypes, antimicrobial resistance, and genetic traits of A. hydrophila isolated from R. dybowskii, providing a theoretical foundation for preventing and controlling A. hydrophila infections.
High HTLV-1 Proviral Load Predates and Predicts HTLV-1-Associated Disease: Literature Review and the London Experience
Authors: Taylor Graham P. , Evans William , Rosadas Carolina
Published: July 2024

Human T cell lymphotropic virus type 1 (HTLV-1) is a retrovirus that infects lymphocytes and causes severe diseases. HTLV-1 proviral load (PVL), i.e., the number of host cells that carry HTLV-1 proviral DNA integrated into their genome, can be measured in peripheral blood mononuclear cells (PBMCs) using quantitative polymerase chain reaction. In this narrative review, we discuss the usefulness of HTLV-1 PVL quantification and share our experience acquired during more than 30 years of follow-up of people living with HTLV-1 in the UK. Patients with HTLV-1-associated myelopathy have higher PVL than those with asymptomatic infection. This is consistent across studies in different countries. High PVL predates symptom onset for both inflammatory and proliferative diseases. High PVL is essential but not sufficient for the development of HTLV-1-associated diseases. Therefore, PVL quantification can be used to support the care of people living with HTLV-1 by identifying those most at risk of HTLV-1-associated diseases.
When Size Matters: New Insights on How Seed Size Can Contribute to the Early Stages of Plant Development
Authors: Boccaccini Alessandra , Cimini Sara , Kazmi Hira , Lepri Andrea , Longo Chiara , Lorrai Riccardo , Vittorioso Paola
Published: July 2024

The seed habit is the most complex and successful method of sexual reproduction in vascular plants. It represents a remarkable moment in the evolution of plants that afterward spread on land. In particular, seed size had a pivotal role in evolutionary success and agronomic traits, especially in the field of crop domestication. Given that crop seeds constitute one of the primary products for consumption, it follows that seed size represents a fundamental determinant of crop yield. This adaptative feature is strictly controlled by genetic traits from both maternal and zygotic tissues, although seed development and growth are also affected by environmental cues. Despite being a highly exploited topic for both basic and applied research, there are still many issues to be elucidated for developmental biology as well as for agronomic science. This review addresses a number of open questions related to cues that influence seed growth and size and how they influence seed germination. Moreover, new insights on the genetic–molecular control of this adaptive trait are presented.
Inheritance of Some Traits in Crosses between Hybrid Tea Roses and Old Garden Roses
Authors: Kılıç Tuğba , Kazaz Soner , Meral Ezgi Doğan , Kırbay Emine
Published: July 2024

The limited knowledge about the inheritance of traits in roses makes the efficient development of rose varieties challenging. In order to achieve breeding goals, the inheritance of traits needs to be explored. Additionally, for the inheritance of a trait like scent, which remains a mystery, it is crucial to know the success of parental traits in transmitting them to the next generation. Understanding this allows for accurate parental selection, ensuring sustainability in meeting market demand and providing convenience to breeders. The aim of this study was to assess the success of cross-combinations between scented old garden roses and hybrid tea roses used in cut roses in transferring their existing traits, with the objective of achieving scented cut roses. The evaluated traits included recurrent blooming, flower stem length, flower diameter, petal number, scent, and bud length of both parents and progenies. The inheritance of these traits was evaluated through theoretical evaluations, including calculating heterosis and heterobeltiosis and determining narrow-sense heritability. The combinations and examined traits were assessed using a hierarchical clustering heat map. The results of this study indicated that flower stem length, flower diameter, petal number, and bud length traits had a moderate degree of narrow-sense heritability, suggesting the influence of non-additive genes on these traits. This study observed a low success rate in obtaining progenies with scent in cross combinations between cut roses and old garden roses, indicating the challenges in obtaining scented genotypes. The discrepancy between the observed phenotypic rates and the expected phenotypic and genotypic rates, according to Punnett squares, suggests that the examined traits could be controlled by polygenic genes. The progenies were observed to exhibit a greater resemblance to old garden roses than hybrid tea roses and did not meet the commercial quality standards for cut flowers. The significant negative heterosis observed in 65.12% (petal number) and 99.61% (flower diameter) of the progenies provides strong evidence of resemblance to old garden roses. Considering these findings, it is recommended to consider old garden roses as parents, taking into account their suitability for other breeding objectives.
Syntaxonomical Remarks on the Garrigues from Apulia (S Italy) and Neighboring Territories
Authors: Tomaselli Valeria , Sciandrello Saverio , Minissale Pietro , Forte Luigi , Costanzo Emanuele , Giusso del Galdo Gianpietro , Carruggio Francesca , Pazienza Gaetano , Brullo Salvatore
Published: July 2024

In this study, the garrigues occurring in Apulia and neighboring territories (southern Italy) were surveyed in order to clarify their syntaxonomical arrangement. Many contributions previously focused on this vegetation type, often adopting different and sometimes contrasting treatments from both the nomenclature and syntaxonomical aspects. Our investigations are supported by the multivariate analysis of a dataset containing 292 phytosociological relevés, whose resulting cluster dendrogram highlights the hierarchical relationships between the examined plant communities. Overall, twenty-one associations with several sub-associations were recognized. Some of them are already known in the literature, whereas others are described here for the first time. As concerns the syntaxonomical framework, this vegetation is attributable to the class Cisto cretici-Micromerietea julianae, which in this territory is represented by the order Cisto eriocephali-Ericetalia manipuliflorae and by two alliances: Cisto eriocephali-Ericion multiflorae, grouping the more thermophilous associations usually distributed along coastlines and at low altitudes; and Cytiso spinescentis-Saturejion montanae, including the mesophilic associations occurring in mountain and sub-mountain belts.
Chemical Profiling of Drimys granadensis (Winteraceae) Essential Oil, and Their Antimicrobial, Antioxidant, and Anticholinesterase Properties
Authors: Cartuche Luis , Vallejo Camila , Castillo Edison , Cumbicus Nixon , Morocho Vladimir
Published: July 2024

A complete and comprehensive chemical and biological study of Drimys granadensis, a native Ecuadorian aromatic plant, was conducted. By conventional steam distillation from dried leaves, a yellowish, translucent essential oil (EO) with a density of 0.95 and a refractive index of 1.5090 was obtained. The EO was analyzed by gas chromatography coupled to a mass spectrometer (GC/MS) and an FID detector (GC/FID), respectively. Enantiomeric distribution was also carried out by GC/MS using a chiral selective column (diethyl tert-butylsilyl-BETA-cyclodextrin). The microdilution broth method was employed to assess the antibacterial and antifungal activity of the EO against a panel of opportunistic microorganisms. Antioxidant capacity was measured using diphenyl picryl hydrazyl (DPPH) and azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals. Finally, the inhibitory potential of the EO against acetylcholinesterase was also valued. Sixty-four chemical compounds, constituting 93.27% of the total composition, were identified, with major components including γ-muurolene (10.63%), spathulenol (10.13%), sabinene (5.52%), and δ-cadinene (4.22%). The characteristic taxonomic marker of the Drimys genus, Drimenol, was detected at very low percentages (<2%). Two pairs of enantiomers ((1S,5R)-(+)-α-pinene/(1S,5S)-(–)-α-pinene; (1S,5R)-(+)-β-pinene/(1S,5S)-(–)-β-pinene) and one pure enantiomer (1R,4S)-(–)-camphene were identified. Regarding antimicrobial potency, the EO exhibited a significant moderate effect on Listeria monocytogenes with a minimal inhibitory concentration (MIC) value of 250 µg/mL, while with the remaining microorganisms, it exerted less potency, ranging from 500 to 2000 µg/mL. The EO displayed moderate effects against the ABTS radical with a half scavenging capacity of 210.48 µg/mL and no effect against the DPPH radical. The most notable effect was noticed for acetylcholinesterase, with a half inhibition concentration (IC50) of 63.88 ± 1.03 µg/mL. These antiradical and anticholinesterase effects hint at potential pharmacological applications in Alzheimer’s disease treatment, although the presence of safrole, albeit in low content (ca. 2%), could limit this opportunity. Further in vivo studies are necessary to fully understand their potential applications.
Exploring Phytoremediation Potential: A Comprehensive Study of Flora Inventory and Soil Heavy Metal Contents in the Northeastern Mining Districts of Morocco
Authors: Oujdi Mohammed , Chafik Yassine , Boukroute Azzouz , Bourgerie Sylvain , Sena-Velez Marta , Morabito Domenico , Addi Mohamed
Published: July 2024

Mining activities produce waste materials and effluents with very high metal concentrations that can negatively impact ecosystems and human health. Consequently, data on soil and plant metal levels are crucial for evaluating pollution severity and formulating soil reclamation strategies, such as phytoremediation. Our research focused on soils and vegetation of a highly contaminated site with potentially toxic metals (Pb, Zn, and Cu) in the Touissit mining districts of eastern Morocco. Vegetation inventory was carried out in three mine tailings of the Touissit mine fields using the “field tower” technique. Here, 91 species belonging to 23 families were inventoried: the most represented families were Poaceae and Asteraceae, and the biological spectrum indicated a predominance of Therophytes (55.12%). From the studied areas, 15 species were selected and collected in triplicate on the tailings and sampled with their corresponding rhizospheric soils, and analyzed for Pb, Zn, and Cu concentrations. Reseda lutea, lotus marocanus, and lotus corniculatus can be considered as hyperaccumulators of Pb, as these plants accumulated more than 1000 mg·kg−1 in their aerial parts. According to TF, these plant species could serve as effective plants for Pb phytoextraction.
Unravelling the Phytochemical Composition and Antioxidant Potential of Different Parts of Rumex vesicarius L.: A RP-HPLC-MS-MS/MS, Chemometrics, and Molecular Docking-Based Comparative Study
Authors: Sweilam Sherouk Hussein , Abd El Hafeez Mohamed S. , Mansour Mahmoud A. , Mekky Reham Hassan
Published: July 2024

Rumex vesicarius L. Polygonaceae is a wildly grown plant in Egypt, North Africa, and Asia with wide traditional uses. Several studies reported its biological activities and richness in phytochemicals. This research addresses a comprehensive metabolic profiling of the flowers, leaves, stems, and roots via RP-HPLC-QTOF-MS and MS/MS with chemometrics. A total of 60 metabolites were observed and grouped into phenolic acids, flavonoids, phenols, terpenes, amino acids, fatty acids, organic acids, and sugars. Principal component analysis and hierarchal cluster analysis showed the segregation of different parts. Moreover, the antioxidant capacity was determined via several methods and agreed with the previous results. Additionally, an in silico approach of molecular docking of the predominant bioactive metabolites was employed against two antioxidant targets, NADPH oxidase and human peroxiredoxin 5 enzyme (PDB ID: 2CDU and 1HD2) receptors, alongside ADME predictions. The molecular modelling revealed that most of the approached molecules were specifically binding with the tested enzymes, achieving high binding affinities. The results confirmed that R. vesicarius stems and roots are rich sources of bioactive antioxidant components. To our knowledge, this is the first comprehensive metabolic profiling of R. vesicarius giving a prospect of its relevance in the development of new naturally based antioxidants.