bluuebunny/crossref_metadata_2025 huggingface dataset:
Meso-Cenozoic Exhumation of the Linqing Sub-Basin, Bohai Bay Basin: Implications for Cratonic Destruction
Authors: Xu Wei , Qiu Nansheng , Chang Jian
Published: October 2021

The relationship between the tectonic event of the Linqing Sub-basin and the destruction of the North China Craton (NCC) is an important factor to consider when studying geodynamic mechanisms in eastern China. In the current study, we present a low-temperature apatite thermochronological analysis of 14 samples to study the tectonic event of the Linqing Sub-basin. Our data showed that the apatite fission track (AFT) ages were in the range of 53.5–124.4 Ma, and the average track lengths were 8.00–11.24 μm. The grain ages showed that 10 samples had mixed ages and were characterized by discordant distribution. The minimum ages decomposed from AFT ages mainly ranged from 105.3 to 40.8 Ma. We identified a break-in-slope from the depth-minimum age profile, which was related to the Meso-Cenozoic tectonic event. The AFT age data could be decomposed into three age groups, namely, P3 (394.8–215.7 Ma), P2 (124.6–83.4 Ma), and P1 (70.7–40.8 Ma), indicating three significant tectonic events in the NCC. P3 is related to the uplift of the NCC at 445.0–315.0 Ma and deformation and magmatism at 320.0–200.0 Ma. P2 corresponds to the Mesozoic tectonic activities, such as the closure of the Mongol–Okhotsk Ocean, the turning of the Izanagi plate and mantle convection. P1 mainly corresponds to the Izanagi–Pacific ridge, the closure of the Tethys Ocean, and the rotation of the Philippine Sea plate in the Cenozoic. Our study provides evidence for the destruction of the NCC, and has significance for the understanding of the deep mechanism.
Nickel Laterites—Mineralogical Monitoring for Grade Definition and Process Optimization
Authors: König Uwe
Published: October 2021

Nickel laterite ore is used to produce nickel metal, predominantly to manufacture stainless steel as well as nickel sulfate, a key ingredient in the batteries that drive electric vehicles. Nickel laterite production is on the rise and surpassing conventional sulfide deposits. The efficiency of mining and processing nickel laterites is defined by their mineralogical composition. Typical profiles of nickel laterites are divided into a saprolite and a laterite horizon. Nickel is mainly concentrated and hosted in a variety of secondary oxides, hydrous Mg silicates and clay minerals like smectite or lizardite in the saprolite horizon, whereas the laterite horizon can host cobalt that could be extracted as a side product. For this case study, 40 samples from both saprolite and laterite horizons were investigated using X-ray diffraction (XRD) in combination with statistical methods such as cluster analysis. Besides the identification of the different mineral phases, the quantitative composition of the samples was also determined with the Rietveld method. Data clustering of the samples was tested and allows a fast and easy separation of the different lithologies and ore grades. Mineralogy also plays a key role during further processing of nickel laterites to nickel metal. XRD was used to monitor the mineralogy of calcine, matte and slag. The value of mineralogical monitoring for grade definition, ore sorting, and processing is explained in the paper.
Virtual and Augmented Reality Direct Ophthalmoscopy Tool: A Comparison between Interactions Methods
Authors: Chan Michael , Uribe-Quevedo Alvaro , Kapralos Bill , Jenkin Michael , Jaimes Norman , Kanev Kamen
Published: October 2021

Direct ophthalmoscopy (DO) is a medical procedure whereby a health professional, using a direct ophthalmoscope, examines the eye fundus. DO skills are in decline due to the use of interactive diagnostic equipment and insufficient practice with the direct ophthalmoscope. To address the loss of DO skills, physical and computer-based simulators have been developed to offer additional training. Among the computer-based simulations, virtual and augmented reality (VR and AR, respectively) allow simulated immersive and interactive scenarios with eye fundus conditions that are difficult to replicate in the classroom. VR and AR require employing 3D user interfaces (3DUIs) to perform the virtual eye examination. Using a combination of a between-subjects and within-subjects paradigm with two groups of five participants, this paper builds upon a previous preliminary usability study that compared the use of the HTC Vive controller, the Valve Index controller, and the Microsoft HoloLens 1 hand gesticulation interaction methods when performing a virtual direct ophthalmoscopy eye examination. The work described in this paper extends our prior work by considering the interactions with the Oculus Quest controller and Oculus Quest hand-tracking system to perform a virtual direct ophthalmoscopy eye examination while allowing us to compare these methods without our prior interaction techniques. Ultimately, this helps us develop a greater understanding of usability effects for virtual DO examinations and virtual reality in general. Although the number of participants was limited, n = 5 for Stage 1 (including the HTC Vive controller, the Valve Index controller, and the Microsoft HoloLens hand gesticulations), and n = 13 for Stage 2 (including the Oculus Quest controller and the Oculus Quest hand tracking), given the COVID-19 restrictions, our initial results comparing VR and AR 3D user interactions for direct ophthalmoscopy are consistent with our previous preliminary study where the physical controllers resulted in higher usability scores, while the Oculus Quest’s more accurate hand motion capture resulted in higher usability when compared to the Microsoft HoloLens hand gesticulation.
Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion
Authors: Alali Aya Q. , Abdal-hay Abdalla , Gulati Karan , Ivanovski Sašo , Fournier Benjamin P. J. , Lee Ryan S. B.
Published: October 2021

Soft tissue integration (STI) at the transmucosal level around dental implants is crucial for the long-term success of dental implants. Surface modification of titanium dental implants could be an effective way to enhance peri-implant STI. The present study aimed to investigate the effect of bioinspired lithium (Li)-doped Ti surface on the behaviour of human gingival fibroblasts (HGFs) and oral biofilm in vitro. HGFs were cultured on various Ti surfaces—Li-doped Ti (Li_Ti), NaOH_Ti and micro-rough Ti (Control_Ti)—and were evaluated for viability, adhesion, extracellular matrix protein expression and cytokine secretion. Furthermore, single species bacteria (Staphylococcus aureus) and multi-species oral biofilms from saliva were cultured on each surface and assessed for viability and metabolic activity. The results show that both Li_Ti and NaOH_Ti significantly increased the proliferation of HGFs compared to the control. Fibroblast growth factor-2 (FGF-2) mRNA levels were significantly increased on Li_Ti and NaOH_Ti at day 7. Moreover, Li_Ti upregulated COL-I and fibronectin gene expression compared to the NaOH_Ti. A significant decrease in bacterial metabolic activity was detected for both the Li_Ti and NaOH_Ti surfaces. Together, these results suggest that bioinspired Li-doped Ti promotes HGF bioactivity while suppressing bacterial adhesion and growth. This is of clinical importance regarding STI improvement during the maintenance phase of the dental implant treatment.
Optimization of Metal-Assisted Chemical Etching for Deep Silicon Nanostructures
Authors: Akan Rabia , Vogt Ulrich
Published: October 2021

High-aspect ratio silicon (Si) nanostructures are important for many applications. Metal-assisted chemical etching (MACE) is a wet-chemical method used for the fabrication of nanostructured Si. Two main challenges exist with etching Si structures in the nanometer range with MACE: keeping mechanical stability at high aspect ratios and maintaining a vertical etching profile. In this work, we investigated the etching behavior of two zone plate catalyst designs in a systematic manner at four different MACE conditions as a function of mechanical stability and etching verticality. The zone plate catalyst designs served as models for Si nanostructures over a wide range of feature sizes ranging from 850 nm to 30 nm at 1:1 line-to-space ratio. The first design was a grid-like, interconnected catalyst (brick wall) and the second design was a hybrid catalyst that was partly isolated, partly interconnected (fishbone). Results showed that the brick wall design was mechanically stable up to an aspect ratio of 30:1 with vertical Si structures at most investigated conditions. The fishbone design showed higher mechanical stability thanks to the Si backbone in the design, but on the other hand required careful control of the reaction kinetics for etching verticality. The influence of MACE reaction kinetics was identified by lowering the oxidant concentration, lowering the processing temperature and by isopropanol addition. We report an optimized MACE condition to achieve an aspect ratio of at least 100:1 at room temperature processing by incorporating isopropanol in the etching solution.
Emerging Applications of Silica Nanoparticles as Multifunctional Modifiers for High Performance Polyester Composites
Authors: Hao Tian , Wang Yao , Liu Zhipeng , Li Jie , Shan Liangang , Wang Wenchao , Liu Jixian , Tang Jianguo
Published: October 2021

Nano-modification of polyester has become a research hotspot due to the growing demand for high-performance polyester. As a functional carrier, silica nanoparticles show large potential in improving crystalline properties, enhancing strength of polyester, and fabricating fluorescent polyester. Herein, we briefly traced the latest literature on synthesis of silica modifiers and the resultant polyester nanocomposites and presented a review. Firstly, we investigated synthesis approaches of silica nanoparticles for modifying polyester including sol-gel and reverse microemulsion technology, and their surface modification methods such as grafting silane coupling agent or polymer. Then, we summarized processing technics of silica-polyester nanocomposites, like physical blending, sol-gel processes, and in situ polymerization. Finally, we explored the application of silica nanoparticles in improving crystalline, mechanical, and fluorescent properties of composite materials. We hope the work provides a guideline for the readers working in the fields of silica nanoparticles as well as modifying polyester.
Thermal Transfer Characteristics of Flat Plate Micro Heat Pipe with Copper Spiral Woven Mesh and a Copper Foam Composite Wick
Authors: Zhang Yanhui , Zhao Zhengang , Luo Chuan , Zhang Dacheng
Published: October 2021

The thermal efficiency limitation of the Flat-plate Micro Heat Pipe (FMHP) is a major challenge in the development of the FMHP, where the effect of wick structure and wettability on its thermal performance is studied to improve the thermal efficiency of the FMHP. In this work, a copper spiral woven mesh and copper foam Composite Wick FMHP (CW-FMHP) is designed based on the conventional Copper Foam Wick FMHP (CFW-FMHP), and its thermal performance is analyzed regarding the wick structure and internal gas–liquid two-phase flow characteristics. An oxidized copper spiral woven mesh and copper foam Composite Wick FMHP (OCW-FMHP) has been further developed through the modification of composite wick wettability. The performance tests are carried out with the thermal transfer characteristics of CW-FMHP, OCW-FMHP, and CFW-FMHP under different filling rates and different thermal powers. The experimental results show that the thermal transfer performance of CW-FMHP reaches the optimal under a liquid filling rate of 150%, where the maximum thermal power is 15.7 W, 35.3% higher than that of the CFW-FMHP under the same filling rate. Moreover, the dynamic response characteristics of the CW-FMHP are significantly improved. The thermal resistance of the CW-FMHP is 0.48 °C/W under the filling rate of 150% at the thermal power of 10 W with a reduction of 9.4% compared to the CFW-FMHP under the same condition. Furthermore, the optimal filling rate for OCW-FMHP is lower compared with the CW-FMHP. The maximum thermal power of OCW-FMHP increases to 17.8 W while the thermal resistance reduces to 0.34 °C/W under the liquid filling rate of 140%. This implies that the composite wick structure designed in this work can improve the thermal transfer performance of the FMHP, and the composite wick with wettability modification is more effective regarding both thermal resistance and maximum thermal power.
Photocatalytic Activity of Titanium Dioxide Nanotubes Following Long-Term Aging
Authors: Abela Stephen , Farrugia Clayton , Xuereb Ryan , Lia Frederick , Zammit Edwin , Rizzo Alex , Refalo Paul , Grech Maurice
Published: October 2021

Anodic titanium dioxide (TiO2) nanotubes were found to be active photocatalysts. These photocatalysts possess a high surface area, even when supported, rendering them potential candidates for water treatment. In this work, photocatalytic surfaces were produced by anodizing commercially pure Ti plates using two different electrolyte compositions and correspondingly diverse process parameters. Changes in the physical and chemical stability as well as photocatalytic activity were studied over a fifty-two-week aging process. During this period, the nanotubular surfaces were exposed to flowing synthetic greywater, solar irradiation, and the natural environment. The physical and phase stability of the materials anodized using the organic electrolyte were found to be outstanding and no degradation or change in crystalline structure was observed. On the other hand, materials anodized in the aqueous electrolyte proved to suffer from light-induced phase transition from anatase to rutile. Surfaces synthesized in the organic electrolyte were more resistant to fouling and showed a better tendency to recover photocatalytic activity upon cleaning. In conclusion, the nanotubes produced in the organic electrolyte proved to be stable, rendering them potentially suitable for real-life applications.
Impact of Controlling a Nutritional Status Score on Wound Healing in Patients with Chronic Limb-Threatening Ischemia after Endovascular Treatment
Authors: Mine Kaori , Sugihara Makoto , Fujita Takafumi , Kato Yuta , Gondo Koki , Arimura Tadaaki , Takamiya Yosuke , Shiga Yuhei , Kuwano Takashi , Miura Shin-ichiro
Published: October 2021

Background: Chronic limb-threatening ischemia (CLTI) is the most advanced stage of peripheral artery disease. Therefore, a multidisciplinary approach is necessary to avoid major amputation in CLTI patients. Malnutrition worsens the condition of CLTI patients, and therefore, it may be important to evaluate the nutritional status in patients with CLTI. This study was designed to evaluate the baseline patient characteristics and the influence of the controlling nutritional status (CONUT) score on the clinical results. Method and Results: A retrospective, single-center, non-randomized study was conducted to evaluate the associations of death, major amputation, and wound healing rate at 12 months with the CONUT score on admission. Consecutive CLTI patients (mean age 73.2 ± 10.4 years; 84 males) who underwent endovascular therapy (EVT) for infra-popliteal lesions at Fukuoka University Hospital from January 2014 to May 2019 were enrolled and divided into two groups (higher and lower CONUT score groups). The higher CONUT group showed a higher percentage of dialysis (66.7% vs. 33.9%, p < 0.001) and a higher clinical frailty scale (5.9 ± 1.4 vs. 4.9 ± 1.9, p = 0.005) than the lower CONUT group. Rates of amputation-free survival were 89.5% and 69.8% in the lower and higher CONUT groups, respectively. In addition, rates of wound healing at 12 months were 98.0% and 78.3% in the lower and higher CONUT groups, respectively. Multivariate regression analysis demonstrated that a higher CONUT score was an independent predictor for delayed wound healing (OR: 11.2; 95% CI: 1.29–97.5; p = 0.028). Conclusion: An assessment of the nutritional status using the CONUT score could be useful for predicting wound healing, and earlier nutritional intervention may improve the outcome of CLTI patients. Early examination and treatment, along with raising awareness of the issue, may be important for improving the prognosis.
Effects of Different Concentrations of Ganpu Tea on Fecal Microbiota and Short Chain Fatty Acids in Mice
Authors: Wang Chen , Gao Zhipeng , Qian Yujiao , Li Xiang , Wang Jieyi , Ma Jie , Guo Jiajing , Fu Fuhua
Published: October 2021

Ganpu tea is composed of tangerine peel and Pu-erh tea. Current research suggests that both products can interact with gut microbes and thus affect health. However, as a kind of compound health food, little information is available about the effect of Ganpu tea on intestinal microorganisms. In this study, the basic physiological parameters (body weight, white adipose tissue and serum fat), the regulation of intestinal microorganisms and content of short-chain fatty acids (SCFAs) in feces of healthy mice were studied. The Ganpu tea can reduce the weight gain of mice and the increase in white adipose tissue (p < 0.01). After the intake of Ganpu tea, the abundance of Bacteroidetes increased (p < 0.05), whereas that of Firmicutes decreased (p < 0.01), indicating the latent capacity of Ganpu tea in adjusting the gut microbiota. Moreover, Ganpu tea differentially affected the content of different types of SCFAs in feces. Ganpu tea at the lowest concentrations showed positive effects on the concentrations of SCFAs such as acetic acid and propionic acid, whereas the concentration of butyric acid was decreased. For branched short-chain fatty acids (BSCFAs) such as isobutyric acid, isovaleric acid, etc., Ganpu tea reduced their concentrations. Our results indicated that Ganpu tea may have positive effects on preventing obesity in humans, but further research is needed before introducing such dietary therapy.
Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding
Authors: Cheema Ali S. , Stinson Lisa F. , Rea Alethea , Lai Ching Tat , Payne Matthew S. , Murray Kevin , Geddes Donna T. , Gridneva Zoya
Published: October 2021

Human milk (HM) components may influence infant growth and development. This study aimed to investigate relationships between infant body composition (BC) and HM lactose, insulin, and glucose (concentrations and calculated daily intakes (CDI)) as well as 24-h milk intake and maternal BC at 3 months postpartum. HM samples were collected at 2 months postpartum. Infant and maternal BC was assessed with bioimpedance spectroscopy. Statistical analysis used linear regression accounting for infant birth weight. 24-h milk intake and CDI of lactose were positively associated with infant anthropometry, lean body mass and adiposity. Higher maternal BC measures were associated with lower infant anthropometry, z-scores, lean body mass, and adiposity. Maternal characteristics including BC and age were associated with concentrations and CDI of HM components, and 24-h milk intake. In conclusion, 24-h intake of HM and lactose as well as maternal adiposity are related to development of infant BC.
Gut Microbiota Reshaped by Pectin Treatment Improves Liver Steatosis in Obese Mice
Authors: Houron Camille , Ciocan Dragos , Trainel Nicolas , Mercier-Nomé Françoise , Hugot Cindy , Spatz Madeleine , Perlemuter Gabriel , Cassard Anne-Marie
Published: October 2021

Pectin, a soluble fiber, improves non-alcoholic fatty-liver disease (NAFLD), but its mechanisms are unclear. We aimed to investigate the role of pectin-induced changes in intestinal microbiota (IM) in NAFLD. We recovered the IM from mice fed a high-fat diet, treated or not with pectin, to perform a fecal microbiota transfer (FMT). Mice fed a high-fat diet, which induces NAFLD, were treated with pectin or received a fecal microbiota transfer (FMT) from mice treated with pectin before (preventive FMT) or after (curative FMT) being fed a high-fat diet. Pectin prevented the development of NAFLD, induced browning of adipose tissue, and modified the IM without increasing the abundance of proteobacteria. Preventive FMT also induced browning of white adipose tissue but did not improve liver steatosis, in contrast to curative FMT, which induced an improvement in steatosis. This was associated with an increase in the concentration of short-chain fatty acids (SCFAs), in contrast to preventive FMT, which induced an increase in the concentration of branched SCFAs. Overall, we show that the effect of pectin may be partially mediated by gut bacteria.
Effects of Nutrition on Cognitive Function in Adults with or without Cognitive Impairment: A Systematic Review of Randomized Controlled Clinical Trials
Authors: Gutierrez Laia , Folch Alexandre , Rojas Melina , Cantero José Luis , Atienza Mercedes , Folch Jaume , Camins Antoni , Ruiz Agustín , Papandreou Christopher , Bulló Mònica
Published: October 2021

New dietary approaches for the prevention of cognitive impairment are being investigated. However, evidence from dietary interventions is mainly from food and nutrient supplement interventions, with inconsistent results and high heterogeneity between trials. We conducted a comprehensive systematic search of randomized controlled trials (RCTs) published in MEDLINE-PubMed, from January 2018 to July 2021, investigating the impact of dietary counseling, as well as food-based and dietary supplement interventions on cognitive function in adults with or without cognitive impairment. Based on the search strategy, 197 eligible publications were used for data abstraction. Finally, 61 articles were included in the analysis. There was reasonable evidence that dietary patterns, as well as food and dietary supplements improved cognitive domains or measures of brain integrity. The Mediterranean diet showed promising results, whereas the role of the DASH diet was not clear. Healthy food consumption improved cognitive function, although the quality of these studies was relatively low. The role of dietary supplements was mixed, with strong evidence of the benefits of polyphenols and combinations of nutrients, but with low evidence for PUFAs, vitamin D, specific protein, amino acids, and other types of supplements. Further well-designed RCTs are needed to guide the development of dietary approaches for the prevention of cognitive impairment.
Brain-Restricted Inhibition of IL-6 Trans-Signaling Mildly Affects Metabolic Consequences of Maternal Obesity in Male Offspring
Authors: Breuer Saida , Kasper Philipp , Vohlen Christina , Janoschek Ruth , Hoffmann Thorben , Appel Sarah , Müller-Limberger Elena , Mesaros Andrea , Rose-John Stefan , Garbers Christoph , Müller Stefan , Lackmann Jan-Wilm , Mahabir Esther , Dötsch Jörg , Hucklenbruch-Rother Eva , Bae-Gartz Inga
Published: October 2021

Maternal obesity greatly affects next generations, elevating obesity risk in the offspring through perinatal programming and flawed maternal and newborn nutrition. The exact underlying mechanisms are poorly understood. Interleukin-6 (IL-6) mediates its effects through a membrane-bound receptor or by trans-signaling (tS), which can be inhibited by the soluble form of the co-receptor gp130 (sgp130). As IL-6 tS mediates western-style diet (WSD) effects via chronic low-grade inflammation (LGI) and LGI is an important mediator in brain–adipose tissue communication, this study aims at determining the effects of maternal obesity in a transgenic mouse model of brain-restricted IL-6tS inhibition (GFAPsgp130) on offspring’s short- and long-term body composition and epigonadal white adipose tissue (egWAT) metabolism. Female wild type (WT) or transgenic mice were fed either standard diet (SD) or WSD pregestationally, during gestation, and lactation. Male offspring received SD from postnatal day (P)21 to P56 and were metabolically challenged with WSD from P56 to P120. At P21, offspring from WT and transgenic dams that were fed WSD displayed increased body weight and egWAT mass, while glucose tolerance testing showed the strongest impairment in GFAPsgp130WSD offspring. Simultaneously, egWAT proteome reveals a characteristic egWAT expression pattern in offspring as a result of maternal conditions. IL-6tS inhibition in transgenic mice was in tendency associated with lower body weight in dams on SD and their respective offspring but blunted by the WSD. In conclusion, maternal nutrition affects offspring’s body weight and egWAT metabolism predominantly independent of IL-6tS inhibition, emphasizing the importance of maternal and newborn nutrition for long-term offspring health.
Lingonberries—General and Oral Effects on the Microbiome and Inflammation
Authors: Pärnänen Pirjo , Lähteenmäki Hanna , Tervahartiala Taina , Räisänen Ismo T. , Sorsa Timo
Published: October 2021

Lingonberry (Vaccinium vitis ideae L.) is a low-bush wild plant found in the northern hemisphere. The berries are used in traditional medicine in Finland to treat oral yeast infections. General and oral effects of lingonberries on the microbiome and inflammation are reviewed. A brief introduction to oral microbiome symbiosis and dysbiosis, innate and adaptive immunity and inflammation are included, and special features in microbe/host interactions in the oral environment are considered. In vitro anticancer, antimicrobial, antioxidant, anti-inflammatory, and in vivo mouse and human studies are included, focusing on the symbiotic effect of lingonberries on oral and general health.
Potential Protective Effects of Equol (Soy Isoflavone Metabolite) on Coronary Heart Diseases—From Molecular Mechanisms to Studies in Humans
Authors: Zhang Xiao , Veliky Cole V. , Birru Rahel L. , Barinas-Mitchell Emma , Magnani Jared W. , Sekikawa Akira
Published: October 2021

Equol, a soy isoflavone-derived metabolite of the gut microbiome, may be the key cardioprotective component of soy isoflavones. Systematic reviews have reported that soy isoflavones have no to very small effects on traditional cardiovascular disease risk factors. However, the potential mechanistic mode of action of equol on non-traditional cardiovascular risk factors has not been systematically reviewed. We searched the PubMed through to July 2021 by using terms for equol and each of the following markers: inflammation, oxidation, endothelial function, vasodilation, atherosclerosis, arterial stiffness, and coronary heart disease. Of the 231 records identified, 69 articles met the inclusion criteria and were summarized. Our review suggests that equol is more lipophilic, bioavailable, and generally more potent compared to soy isoflavones. Cell culture, animal, and human studies show that equol possesses antioxidative, anti-inflammatory, and vasodilatory properties and improves arterial stiffness and atherosclerosis. Many of these actions are mediated through the estrogen receptor β. Overall, equol may have a greater cardioprotective benefit than soy isoflavones. Clinical studies of equol are warranted because equol is available as a dietary supplement.
Therapeutic Potential of Mitophagy-Inducing Microflora Metabolite, Urolithin A for Alzheimer’s Disease
Authors: Jayatunga Dona Pamoda W. , Hone Eugene , Khaira Harjot , Lunelli Taciana , Singh Harjinder , Guillemin Gilles J. , Fernando Binosha , Garg Manohar L. , Verdile Giuseppe , Martins Ralph N.
Published: October 2021

Mitochondrial dysfunction including deficits of mitophagy is seen in aging and neurodegenerative disorders including Alzheimer’s disease (AD). Apart from traditionally targeting amyloid beta (Aβ), the main culprit in AD brains, other approaches include investigating impaired mitochondrial pathways for potential therapeutic benefits against AD. Thus, a future therapy for AD may focus on novel candidates that enhance optimal mitochondrial integrity and turnover. Bioactive food components, known as nutraceuticals, may serve as such agents to combat AD. Urolithin A is an intestinal microbe-derived metabolite of a class of polyphenols, ellagitannins (ETs). Urolithin A is known to exert many health benefits. Its antioxidant, anti-inflammatory, anti-atherogenic, anti-Aβ, and pro-mitophagy properties are increasingly recognized. However, the underlying mechanisms of urolithin A in inducing mitophagy is poorly understood. This review discusses the mitophagy deficits in AD and examines potential molecular mechanisms of its activation. Moreover, the current knowledge of urolithin A is discussed, focusing on its neuroprotective properties and its potential to induce mitophagy. Specifically, this review proposes potential mechanisms by which urolithin A may activate and promote mitophagy.
Factors Associated with Serum Vitamin D Metabolites and Vitamin D Metabolite Ratios in Premenopausal Women
Authors: Toribio María José , Priego-Capote Feliciano , Pérez-Gómez Beatriz , Fernández de Larrea-Baz Nerea , Ruiz-Moreno Emma , Castelló Adela , Lucas Pilar , Sierra María Ángeles , Pino Marina Nieves , Martínez-Cortés Mercedes , Luque de Castro María Dolores , Lope Virginia , Pollán Marina
Published: October 2021

The most representative indicator of vitamin D status in clinical practice is 25(OH)D3, but new biomarkers could improve the assessment of vitamin D status and metabolism. The objective of this study is to investigate the association of serum vitamin D metabolites and vitamin D metabolite ratios (VMRs) with potentially influential factors in premenopausal women. This is a cross-sectional study based on 1422 women, aged 39–50, recruited from a Madrid Medical Diagnostic Center. Participants answered an epidemiological and a food frequency questionnaire. Serum vitamin D metabolites were determined using an SPE–LC–MS/MS platform. The association between participant’s characteristics, vitamin D metabolites, and VMRs was quantified by multiple linear regression models. Mean 25(OH)D3 concentration was 49.2 + 18.9 nmol/L, with greater deficits among obese, nulliparous, dark-skinned women, and with less sun exposure. A lower R2 ratio (1,25(OH)2D3/25(OH)D3) and a higher R4 (24,25(OH)2D3/1,25(OH)2D3) were observed in nulliparous women, with high sun exposure, and those with low caloric intake or high consumption of calcium, vitamin D supplements, or alcohol. Nulliparous women had lower R1 (25(OH)D3/Vit D3) and R3 (24,25(OH)2D3/25(OH)D3), and older women showed lower R3 and R4. Vitamin D status modified the association of the VMRs with seasons. VMRs can be complementary indicators of vitamin D status and its endogenous metabolism, and reveal the influence of certain individual characteristics on the expression of hydroxylase enzymes.
Kaempferol, Myricetin and Fisetin in Prostate and Bladder Cancer: A Systematic Review of the Literature
Authors: Crocetto Felice , di Zazzo Erika , Buonerba Carlo , Aveta Achille , Pandolfo Savio Domenico , Barone Biagio , Trama Francesco , Caputo Vincenzo Francesco , Scafuri Luca , Ferro Matteo , Cosimato Vincenzo , Fusco Ferdinando , Imbimbo Ciro , Di Lorenzo Giuseppe
Published: October 2021

Prostate and bladder cancer represent the two most frequently diagnosed genito-urinary malignancies. Diet has been implicated in both prostate and bladder cancer. Given their prolonged latency and high prevalence rates, both prostate and bladder cancer represent attractive candidates for dietary preventive measures, including the use of nutritional supplements. Flavonols, a class of flavonoids, are commonly found in fruit and vegetables and are known for their protective effect against diabetes and cardiovascular diseases. Furthermore, a higher dietary intake of flavonols was associated with a lower risk of both bladder and prostate cancer in epidemiological studies. In this systematic review, we gathered all available evidence supporting the anti-cancer potential of selected flavonols (kaempferol, fisetin and myricetin) against bladder and prostate cancer. A total of 21, 15 and 7 pre-clinical articles on bladder or prostate cancer reporting on kaempferol, fisetin and myricetin, respectively, were found, while more limited evidence was available from animal models and epidemiological studies or clinical trials. In conclusion, the available evidence supports the potential use of these flavonols in prostate and bladder cancer, with a low expected toxicity, thus providing the rationale for clinical trials that explore dosing, settings for clinical use as well as their use in combination with other pharmacological and non-pharmacological interventions.
Randomized Controlled Trial for Promotion of Healthy Eating in Older Adults by Increasing Consumption of Plant-Based Foods: Effect on Inflammatory Biomarkers
Authors: Nilsson Andreas , Cano Antonio , Bergens Oscar , Kadi Fawzi
Published: October 2021

To what extent the intake of fruit and vegetables (FV) influences inflammatory status remains elusive, particularly in older populations. The aim of the present study was to determine the effect of increased FV intake for 16 weeks on circulating biomarkers of inflammation in a population of older men and women. Sixty-six participants (65–70 years) randomly assigned to either FV or control (CON) groups were instructed to increase FV intake to five servings per day through nutritional counseling (FV) or to maintain habitual diet (CON). Dietary intake and physical activity level (PA) were determined using food frequency questionnaire and accelerometers, respectively, at the start and end of the intervention. C-reactive protein (CRP), interleukin 6 (IL-6), IL-18, macrophage inflammatory protein-1α (MIP-1α), MIP-1β, tumor necrosis factor-α (TNF-α), TNF-related apoptosis-inducing ligand (TRAIL), TNF-related activation-induced cytokine (TRANCE), and C-X3-C motif chemokine ligand-1 (CX3CL1, or fractalkine) were analyzed. The FV group significantly increased daily FV intake (from 2.2 ± 1.3 to 4.2 ± 1.8 servings/day), with no change in CON. Waist circumference and PA level were unchanged by the intervention. Interaction effects (time × group, p < 0.05) for TRAIL, TRANCE, and CX3CL1 denoting a significant decrease (p < 0.05) in FV but not in CON were observed. No corresponding effects on CRP, IL6, TNF-α, MIP-1α, and β and IL-18 were observed. The present study demonstrates the influence of increased FV consumption on levels of some inflammatory biomarkers in a population of older adults. Future work is warranted to examine the clinical implications of FV-induced alterations in these inflammatory biomarkers.
Relative Validity of a Short 15-Item Food Frequency Questionnaire Measuring Dietary Quality, by the Diet History Method
Authors: Rothenberg Elisabet , Strandhagen Elisabeth , Samuelsson Jessica , Ahlner Felicia , Rydberg Sterner Therese , Skoog Ingmar , Lundberg Christina E.
Published: October 2021

Food frequency questionnaires (FFQ) are commonly used dietary assessment tools. The aim was to assess the relative validity of a 15-item FFQ, designed for the screening of poor dietary patterns with a validated diet history (DH). The study population was derived from the Gothenburg H70 Birth Cohort Studies. The DH registrations were harmonized in accordance with the FFQ frequencies. The agreement was assessed by Cohen’s kappa with corresponding confidence intervals (CI) for the frequency and categorical variables. Bland–Altman plots were used for the numeric variables. The study comprised data from 848 individuals (55.2% women). Overall, there was high agreement between the methods, with the exact and adjacent level of agreement over 80% for eight variables. The proportion attributed to the opposite frequency was fairly low for most of the frequency variables. Most of the kappa values were in fair or moderate agreement. The highest kappa values were calculated for the type of cooking fat (k = 0.68, CI = 0.63–0.72) and sandwich spread (k = 0.55, CI = 0.49–0.53), and the lowest for type of bread (0.13, CI = 0.07–0.20) and sweets (0.22 CI = 0.18–0.27). In conclusion, the FFQ showed overall good agreement compared with the DH. We, therefore, think it, with some improvements, could serve as a simple screening tool for poor dietary patterns.
A Scoping Review of the Application of Metabolomics in Nutrition Research: The Literature Survey 2000–2019
Authors: Shibutami Eriko , Takebayashi Toru
Published: October 2021

Nutrimetabolomics is an emerging field in nutrition research, and it is expected to play a significant role in deciphering the interaction between diet and health. Through the development of omics technology over the last two decades, the definition of food and nutrition has changed from sources of energy and major/micro-nutrients to an essential exposure factor that determines health risks. Furthermore, this new approach has enabled nutrition research to identify dietary biomarkers and to deepen the understanding of metabolic dynamics and the impacts on health risks. However, so far, candidate markers identified by metabolomics have not been clinically applied and more efforts should be made to validate those. To help nutrition researchers better understand the potential of its application, this scoping review outlined the historical transition, recent focuses, and future prospects of the new realm, based on trends in the number of human research articles from the early stage of 2000 to the present of 2019 by searching the Medical Literature Analysis and Retrieval System Online (MEDLINE). Among them, objective dietary assessment, metabolic profiling, and health risk prediction were positioned as three of the principal applications. The continued growth will enable nutrimetabolomics research to contribute to personalized nutrition in the future.
A Comparison of Different Approaches to Clinical Phenotyping of Lithium Response: A Proof of Principle Study Employing Genetic Variants of Three Candidate Circadian Genes
Authors: Scott Jan , Lajnef Mohamed , Icick Romain , Bellivier Frank , Marie-Claire Cynthia , Etain Bruno
Published: October 2021

Optimal classification of the response to lithium (Li) is crucial in genetic and biomarker research. This proof of concept study aims at exploring whether different approaches to phenotyping the response to Li may influence the likelihood of detecting associations between the response and genetic markers. We operationalized Li response phenotypes using the Retrospective Assessment of Response to Lithium Scale (i.e., the Alda scale) in a sample of 164 cases with bipolar disorder (BD). Three phenotypes were defined using the established approaches, whilst two phenotypes were generated by machine learning algorithms. We examined whether these five different Li response phenotypes showed different levels of statistically significant associations with polymorphisms of three candidate circadian genes (RORA, TIMELESS and PPARGC1A), which were selected for this study because they were plausibly linked with the response to Li. The three original and two revised Alda ratings showed low levels of discordance (misclassification rates: 8–12%). However, the significance of associations with circadian genes differed when examining previously recommended categorical and continuous phenotypes versus machine-learning derived phenotypes. Findings using machine learning approaches identified more putative signals of the Li response. Established approaches to Li response phenotyping are easy to use but may lead to a significant loss of data (excluding partial responders) due to recent attempts to improve the reliability of the original rating system. While machine learning approaches require additional modeling to generate Li response phenotypes, they may offer a more nuanced approach, which, in turn, would enhance the probability of identifying significant signals in genetic studies.
Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
Authors: Ustyuzhanina Nadezhda E. , Anisimova Natalia Yu. , Bilan Maria I. , Donenko Fedor V. , Morozevich Galina E. , Yashunskiy Dmitriy V. , Usov Anatolii I. , Siminyan Nara G. , Kirgisov Kirill I. , Varfolomeeva Svetlana R. , Kiselevskiy Mikhail V. , Nifantiev Nikolay E.
Published: October 2021

The immunosuppression and inhibition of hematopoiesis are considered to be reasons for the development of complications after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation. Chondroitin sulfate (CS), isolated from the fish Salmo salar, and fucosylated chondroitin sulfate (FCS), isolated from the sea cucumber Apostichopus japonicus, were studied for their roles as stimulators of hematopoiesis in a model of cyclophosphamide-induced immunosuppression in mice. The recombinant protein r G-CSF was applied as a reference. The studied polysaccharides were shown to stimulate the release of white and red blood cells, as well as platelets from bone marrow in immunosuppressed mice, while r G-CSF was only responsible for the significant increase in the level of leucocytes. The analysis of different populations of leucocytes in blood indicated that r G-CSF mainly stimulated the production of neutrophils, whereas in the cases of the studied saccharides, increases in the levels of monocytes, lymphocytes and neutrophils were observed. The normalization of the level of the pro-inflammatory cytokine IL-6 in the serum and the recovery of cell populations in the spleen were observed in immunosuppressed mice following treatment with the polysaccharides. An increase in the proliferative activity of hematopoietic cells CD34(+)CD45(+) was observed following ex vivo polysaccharide exposure. Further study on related oligosaccharides regarding their potential as promising drugs in the complex prophylaxis and therapy of hematopoiesis inhibition after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation seems to be warranted.
On the Mechanism and Kinetics of Synthesizing Polymer Nanogels by Ionizing Radiation-Induced Intramolecular Crosslinking of Macromolecules
Authors: Ashfaq Aiysha , An Jung-Chul , Ulański Piotr , Al-Sheikhly Mohamad
Published: October 2021

Nanogels—internally crosslinked macromolecules—have a growing palette of potential applications, including as drug, gene or radioisotope nanocarriers and as in vivo signaling molecules in modern diagnostics and therapy. This has triggered considerable interest in developing new methods for their synthesis. The procedure based on intramolecular crosslinking of polymer radicals generated by pulses of ionizing radiation has many advantages. The substrates needed are usually simple biocompatible polymers and water. This eliminates the use of monomers, chemical crosslinking agents, initiators, surfactants, etc., thus limiting potential problems with the biocompatibility of products. This review summarizes the basics of this method, providing background information on relevant aspects of polymer solution thermodynamics, radiolysis of aqueous solutions, generation and reactions of polymer radicals, and the non-trivial kinetics and mechanism of crosslinking, focusing on the main factors influencing the outcomes of the radiation synthesis of nanogels: molecular weight of the starting polymer, its concentration, irradiation mode, absorbed dose of ionizing radiation and temperature. The most important techniques used to perform the synthesis, to study the kinetics and mechanism of the involved reactions, and to assess the physicochemical properties of the formed nanogels are presented. Two select important cases, the synthesis of nanogels based on polyvinylpyrrolidone (PVP) and/or poly(acrylic acid) (PAA), are discussed in more detail. Examples of recent application studies on radiation-synthesized PVP and PAA nanogels in transporting drugs across the blood–brain barrier and as targeted radioisotope carriers in nanoradiotherapy are briefly described.
Generation of Photonic Hooks from Patchy Microcylinders
Authors: Tang Fen , Shang Qingqing , Yang Songlin , Wang Ting , Melinte Sorin , Zuo Chao , Ye Ran
Published: October 2021

The photonic hook (PH) is a new type of curved light beam, which has promising applications in various fields such as nanoparticle manipulation, super-resolution imaging, and so forth. Herein, we proposed a new approach of utilizing patchy microcylinders for the generation of PHs. Numerical simulation based on the finite-difference time-domain method was used to investigate the field distribution characteristics of the PHs. By rotating the patchy microcylinder, PHs with different curvatures can be effectively generated, and the PH with a bending angle of 28.4∘ and a full-width-half-maximum of 0.36 λ can be obtained from 1 μm-diameter patchy microcylinders.
Effects of Sub-Micro Sized BaTiO3 Blocking Particles and Ag-Deposited Nano-Sized BaTiO3 Hybrid Particles on Dielectric Properties of Poly(vinylidene-fluoride) Polymer
Authors: Silakaew Kanyapak , Thongbai Prasit
Published: October 2021

This work provided an alternative route to balance the significantly increased dielectric permittivity (ε′) and effectively retained tanδ using an effective two-step concept. Ag-deposited nano-sized BaTiO3 (Ag-nBT) hybrid particle was used as the first filler to increase the ε′ of the poly(vinylidene-fluoride) (PVDF) polymer via the strong interfacial polarization and a high permittivity of nBT and suppress the increased loss tangent (tanδ) owing to the discrete growth of Ag nanoparticles on the surface of nBT, preventing a continuous percolating path. The ε′ and tanδ values at 103 Hz of the Ag-nBT/PVDF composite with fAg-nBT~0.29 were 61.7 and 0.036. The sub-micron-sized BaTiO3 (μBT) particle was selected as the blocking particles to doubly reduce the tanδ with simultaneously enhanced ε′ due to the presence of the tetragonal BT phase. The μBT blocking particles can effectively further inhibit the formation of conducting network and hence further reducing tanδ. By incorporation of μBT clocking particles with fμBT = 0.2, the ε′ value of the Ag-nBT/PVDF-μBT composite (fAg-nBT = 0.30) can significantly increase to 161.4, while the tanδ was reduced to 0.026. Furthermore, the tanδ was lower than 0.09 in the temperature range of −60–150 °C due to the blocking effect of μBT particles.
Application of Wavelet Feature Extraction and Artificial Neural Networks for Improving the Performance of Gas–Liquid Two-Phase Flow Meters Used in Oil and Petrochemical Industries
Authors: Hosseini Siavash , Taylan Osman , Abusurrah Mona , Akilan Thangarajah , Nazemi Ehsan , Eftekhari-Zadeh Ehsan , Bano Farheen , Roshani Gholam Hossein
Published: October 2021

Measuring fluid characteristics is of high importance in various industries such as the polymer, petroleum, and petrochemical industries, etc. Flow regime classification and void fraction measurement are essential for predicting the performance of many systems. The efficiency of multiphase flow meters strongly depends on the flow parameters. In this study, MCNP (Monte Carlo N-Particle) code was employed to simulate annular, stratified, and homogeneous regimes. In this approach, two detectors (NaI) were utilized to detect the emitted photons from a cesium-137 source. The registered signals of both detectors were decomposed using a discrete wavelet transform (DWT). Following this, the low-frequency (approximation) and high-frequency (detail) components of the signals were calculated. Finally, various features of the approximation signals were extracted, using the average value, kurtosis, standard deviation (STD), and root mean square (RMS). The extracted features were thoroughly analyzed to find those features which could classify the flow regimes and be utilized as the inputs to a network for improving the efficiency of flow meters. Two different networks were implemented for flow regime classification and void fraction prediction. In the current study, using the wavelet transform and feature extraction approach, the considered flow regimes were classified correctly, and the void fraction percentages were calculated with a mean relative error (MRE) of 0.4%. Although the system presented in this study is proposed for measuring the characteristics of petroleum fluids, it can be easily used for other types of fluids such as polymeric fluids.