bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (FAD) Genes in Camelina sativa (L.) Crantz
Authors: Sun Daqian , Quan Weizhu , Wang Di , Cui Jingyan , Wang Tianyi , Lin Mei , Wang Yijin , Wang Nan , Dong Yuanyuan , Li Xiaowei , Liu Weican , Wang Fawei
Published: November 2022

Camelina sativa (L.) Crantz is an indispensable oilseed crop, and its seeds contain many unsaturated fatty acids. FAD (fatty acid desaturase) regulates the synthesis of unsaturated fatty acids. In this research, we performed CsFAD gene family analysis and identified 24 CsFAD genes in Camelina, which were unevenly distributed on 14 of the 19 total chromosomes. Phylogenetic analysis showed that CsFAD includes four subfamilies, supported by the conserved structures and motifs of CsFAD genes. In addition, we investigated the expression patterns of the FAD family in the different tissues of Camelina. We found that CsFAD family genes were all expressed in the stem, and CsFAD2-2 was highly expressed in the early stage of seed development. Moreover, during low temperature (4 °C) stress, we identified that the expression level of CsFAD2-2 significantly changed. By observing the transient expression of CsFAD2-2 in Arabidopsis protoplasts, we found that CsFAD2-2 was located on the nucleus. Through the detection and analysis of fatty acids, we prove that CsFAD2-2 is involved in the synthesis of linolenic acid (C18:3). In conclusion, we identified CsFAD2-2 through the phylogenetic analysis of the CsFAD gene family and further determined the fatty acid content to find that CsFAD2-2 is involved in fatty acid synthesis in Camelina.
Use of Hierarchical Carbon Nanofibers Decorated with NiCo Nanoparticles for Highly Sensitive Vortioxetine Determination
Authors: Smajdor Joanna , Zambrzycki Marcel , Paczosa-Bator Beata , Piech Robert
Published: November 2022

A new voltammetry method for the highly sensitive antidepressant drug vortioxetine (VOR) is presented using glassy carbon electrodes modified with hierarchical carbon nanofibers with NiCo nanoparticles (eCNF/CNT/NiCo-GCE). The electrochemical behavior of VOR was investigated by cyclic voltammetry, which indicates that its oxidation is an adsorption-controlled process with the exchange of two electrons and one proton. The effects of various factors on the VOR peak, such as supporting electrolyte type, preconcentration time, and potential, or influence of interferents, were tested using the square wave voltammetry technique (SWV). The linear voltametric response for the analyte was obtained in the concentration range from 0.01·10−6 to 3.0·10−6 mol L−1 with the detection limit of 1.55·10−9 mol L−1 for a preconcentration time of 60 s. The proposed method was successfully applied for highly sensitive VOR determination in complex matrices such as tablets, urine, and plasma with good recovery parameter.
Adrenomedullin Mitigates Doxorubicin-Induced Nephrotoxicity in Rats: Role of Oxidative Stress, Inflammation, Apoptosis, and Pyroptosis
Authors: Abd-Ellatif Rania Nagi , Nasef Nahla Anas , El-Horany Hemat El-Sayed , Emam Marwa Nagy , Younis Reham Lotfy , El Gheit Rehab E. Abo , Elseady Walaa , Radwan Doaa A. , Hafez Yasser Mostafa , Eissa Ahmad , Aboalsoud Alshimaa , Shalaby Rania H. , Atef Marwa Mohamed
Published: November 2022

Doxorubicin (DOX) is an anticancer antibiotic which has various effects in human cancers. It is one of the commonly known causes of drug-induced nephrotoxicity, which results in acute renal injury. Adrenomedullin (ADM), a vasodilator peptide, is widely distributed in many tissues and has potent protective effects. Therefore, the current study aimed to examine the protective potential mechanisms of ADM against DOX-induced nephrotoxicity. A total of 28 male Wistar rats were randomized into four groups: control group, doxorubicin group (15 mg/kg single intraperitoneal injection of DOX), adrenomedullin + doxorubicin group (12 μg/kg/day intraperitoneal injection of ADM) 3 days prior to DOX injection and continuing for 14 days after the model was established, and adrenomedullin group. Kidney function biomarkers, oxidative stress markers, and inflammatory mediators (TNF-α, NLRP3, IL-1β, and IL-18) were assessed. The expressions of gasdermin D and ASC were assessed by real-time PCR. Furthermore, the abundances of caspase-1 (p20), Bcl-2, and Bax immunoreactivity were evaluated. ADM administration improved the biochemical parameters of DOX-induced nephrotoxicity, significantly reduced oxidative damage markers and inflammatory mediators, and suppressed both apoptosis and pyroptosis. These results were confirmed by the histopathological findings and revealed that ADM’s antioxidant, anti-inflammatory, anti-apoptotic, and anti-pyroptotic properties may have prospective applications in the amelioration of DOX-induced nephrotoxicity.
Knockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development
Authors: Dong Yazheng , Wu Xiao , Peng Xitong , Yang Liusong , Tan Baohua , Zhao Huaxing , Zheng Enqin , Hong Linjun , Cai Gengyuan , Wu Zhenfang , Li Zicong
Published: November 2022

The technique of cloning has wide applications in animal husbandry and human biomedicine. However, the very low developmental efficiency of cloned embryos limits the application of cloning. Ectopic XIST-expression-induced abnormal X chromosome inactivation (XCI) is a primary cause of the low developmental competence of cloned mouse and pig embryos. Knockout or knockdown of XIST improves cloning efficiency in both pigs and mice. The transcription factor Yin yang 1(YY1) plays a critical role in XCI by triggering the transcription of X-inactive specific transcript (XIST) and facilitating the localization of XIST RNA on the X chromosome. This study aimed to investigate whether RNA interference to suppress the expression of YY1 can inhibit erroneous XIST expression, rescue abnormal XCI, and improve the developmental ability of cloned pig embryos. The results showed that YY1 binds to the 5′ regulatory region of the porcine XIST gene in pig cells. The microinjection of YY1 siRNA into cloned pig embryos reduced the transcript abundance of XIST and upregulated the mRNA level of X-linked genes at the 4-cell and blastocyst stages. The siRNA-mediated knockdown of YY1 altered the transcriptome and enhanced the in vitro and in vivo developmental efficiency of cloned porcine embryos. These results suggested that YY1 participates in regulating XIST expression and XCI in cloned pig embryos and that the suppression of YY1 expression can increase the developmental rate of cloned pig embryos. The present study established a new method for improving the efficiency of pig cloning.
Cooling-Rate Computer Simulations for the Description of Crystallization of Organic Phase-Change Materials
Authors: Nazarychev Victor M. , Glova Artyom D. , Larin Sergey V. , Lyulin Alexey V. , Lyulin Sergey V. , Gurtovenko Andrey A.
Published: November 2022

A molecular-level insight into phase transformations is in great demand for many molecular systems. It can be gained through computer simulations in which cooling is applied to a system at a constant rate. However, the impact of the cooling rate on the crystallization process is largely unknown. To this end, here we performed atomic-scale molecular dynamics simulations of organic phase-change materials (paraffins), in which the cooling rate was varied over four orders of magnitude. Our computational results clearly show that a certain threshold (1.2 × 1011 K/min) in the values of cooling rates exists. When cooling is slower than the threshold, the simulations qualitatively reproduce an experimentally observed abrupt change in the temperature dependence of the density, enthalpy, and thermal conductivity of paraffins upon crystallization. Beyond this threshold, when cooling is too fast, the paraffin’s properties in simulations start to deviate considerably from experimental data: the faster the cooling, the larger part of the system is trapped in the supercooled liquid state. Thus, a proper choice of a cooling rate is of tremendous importance in computer simulations of organic phase-change materials, which are of great promise for use in domestic heat storage devices.
Therapeutic Antibodies in Cancer Treatment in the UK
Authors: Eltarhoni Khadiga , Kamel Faddy , Ihebunezie Katrina , Nisar Pasha , Soloviev Mikhail
Published: November 2022

The growing understanding of the molecular mechanisms of carcinogenesis accelerated the development of monoclonal therapeutic antibodies to specifically target multiple cancer pathways. Recombinant protein therapeutics now constitute a large proportion of yearly approved medicines. Oncology, autoimmune diseases and to a smaller degree the prophylaxis of organ transplant rejection are their main application areas. As of the date of this review, 37 monoclonal antibody products are approved for use in cancer treatments in the United Kingdom. Currently, the antibody therapeutics market is dominated by monoclonal immunoglobulins (IgGs). New types of recombinant antibody therapeutics developed more recently include bispecific recombinant antibodies and other recombinantly produced functional proteins. This review focuses on the approved therapeutic antibodies used in cancer treatment in the UK today and describes their antigen targets and molecular mechanisms involved. We provide convenient links to the relevant databases and other relevant resources for all antigens and antibodies mentioned. This review provides a comprehensive summary of the different monoclonal antibodies that are currently in clinical use primarily in malignancy, including their function, which is of importance to those in the medical field and allied specialties.
E3 Ubiquitin Ligases: The Operators of the Ubiquitin Code That Regulates the RLR and cGAS-STING Pathways
Authors: Ji Likai , Wang Yan , Zhou Liying , Lu Juan , Bao Siwen , Shen Quan , Wang Xiaochun , Liu Yuwei , Zhang Wen
Published: November 2022

The outbreaks caused by RNA and DNA viruses, such as SARS-CoV-2 and monkeypox, pose serious threats to human health. The RLR and cGAS-STING pathways contain major cytoplasmic sensors and signaling transduction axes for host innate antiviral immunity. In physiological and virus-induced pathological states, the activation and inactivation of these signal axes are tightly controlled, especially post-translational modifications (PTMs). E3 ubiquitin ligases (E3s) are the direct manipulator of ubiquitin codons and determine the type and modification type of substrate proteins. Therefore, members of the E3s family are involved in balancing the host’s innate antiviral immune responses, and their functions have been extensively studied over recent decades. In this study, we overviewed the mechanisms of different members of three E3s families that mediate the RLR and cGAS-STING axes and analyzed them as potential molecular targets for the prevention and treatment of virus-related diseases.
Spatial-Controlled Coating of Pro-Angiogenic Proteins on 3D Porous Hydrogels Guides Endothelial Cell Behavior
Authors: Le Bao Chau , Waller Helen , Dellaquila Alessandra , Peters Daniel , Lakey Jeremy , Chaubet Frédéric , Simon-Yarza Teresa
Published: November 2022

In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function. The microenvironment of the ECM plays a fundamental role in regulating angiogenesis. Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems. The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior. Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF). Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)–dextran. Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules. Cells in functionalized scaffolds survived, adhered, and proliferated over seven days. When incorporated alone, Caf1-YIGSR mainly induced cell adhesion and proliferation, whereas Caf1-VEGF promoted cell migration and sprouting. Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective. This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials.
Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes
Authors: Li Zhonghao , Zhang Qi , Liu Xianyan , Zhao Ming
Published: November 2022

Hyperlipidemia-associated lipid disorders are considered the cause of atherosclerotic cardiovascular disease. Reverse cholesterol transport (RCT) is a mechanism by which excess peripheral cholesterol is transported to the liver and further converted into bile acid for excretion from the body in feces, which contributes to reducing hyperlipidemia as well as cardiovascular disease. We previously found that the recombinant humanized IgG1 antibody promotes macrophages to engulf lipids and increases cholesterol efflux to high-density lipoprotein (HDL) through ATP-binding cassette sub-family A1 (ABCA1), one of the key proteins related to RCT. In the present study, we explored other RCT related proteins expression on hepatocytes, including scavenger receptor class B type I (SR-BI), apolipoprotein A-I (ApoA-I), and apolipoprotein A-II (ApoA-II), and its modulation mechanism involved. We confirmed that the recombinant humanized IgG1 antibody selectively activated ERK1/2 to upregulate SR-BI, ApoA-I, and ApoA-II expression in mice liver and human hepatocellular carcinoma cell lines HepG2 cells. The rate-limiting enzymes of bile acid synthesis, including cholesterol 7α-hydroxylase (CYP7A1) and sterol 27-hydroxylase (CYP27A1), exhibited a significant increase when treated with the recombinant humanized IgG1 antibody, as well as increased excretion of bile acids in feces. Besides, abolishment or mutation of peroxisome proliferator-activated receptor α (PPARα)/RXR binding site on SR-BI promoter eliminated SR-BI reporter gene luciferase activity even in the presence of the recombinant humanized IgG1 antibody. Knock down the neonatal Fc receptor (FcRn) on hepatocytes impaired the effect of recombinant humanized IgG1 antibody on activation of ERK1/2, as well as upregulation of SR-BI, ApoA-I, and ApoA-II expression. In conclusion, one of the mechanisms on the recombinant humanized IgG1 antibody attenuates hyperlipidemia in ApoE−/− mice model fed with high-fat-diet might be through reinforcement of liver RCT function in an FcRn-ERK1/2-PPARα dependent manner.
3D Printing in Regenerative Medicine: Technologies and Resources Utilized
Authors: Kantaros Antreas
Published: November 2022

Over the past ten years, the use of additive manufacturing techniques, also known as “3D printing”, has steadily increased in a variety of scientific fields. There are a number of inherent advantages to these fabrication methods over conventional manufacturing due to the way that they work, which is based on the layer-by-layer material-deposition principle. These benefits include the accurate attribution of complex, pre-designed shapes, as well as the use of a variety of innovative raw materials. Its main advantage is the ability to fabricate custom shapes with an interior lattice network connecting them and a porous surface that traditional manufacturing techniques cannot adequately attribute. Such structures are being used for direct implantation into the human body in the biomedical field in areas such as bio-printing, where this potential is being heavily utilized. The fabricated items must be made of biomaterials with the proper mechanical properties, as well as biomaterials that exhibit characteristics such as biocompatibility, bioresorbability, and biodegradability, in order to meet the strict requirements that such procedures impose. The most significant biomaterials used in these techniques are listed in this work, but their advantages and disadvantages are also discussed in relation to the aforementioned properties that are crucial to their use.
Never, Ever Make an Enemy… Out of an Anemone: Transcriptomic Comparison of Clownfish Hosting Sea Anemone Venoms
Authors: Delgado Alonso , Benedict Charlotte , Macrander Jason , Daly Marymegan
Published: November 2022

Sea anemones are predatory marine invertebrates and have diverse venom arsenals. Venom is integral to their biology, and is used in competition, defense, and feeding. Three lineages of sea anemones are known to have independently evolved symbiotic relationships with clownfish, however the evolutionary impact of this relationship on the venom composition of the host is still unknown. Here, we investigate the potential of this symbiotic relationship to shape the venom profiles of the sea anemones that host clownfish. We use transcriptomic data to identify differences and similarities in venom profiles of six sea anemone species, representing the three known clades of clownfish-hosting sea anemones. We recovered 1121 transcripts matching verified toxins across all species, and show that hemolytic and hemorrhagic toxins are consistently the most dominant and diverse toxins across all species examined. These results are consistent with the known biology of sea anemones, provide foundational data on venom diversity of these species, and allow for a review of existing hierarchical structures in venomic studies.
Phytochemical Analysis and Habitat Suitability Mapping of Cardiocrinum cordatum (Thunb.) Makino Collected at Chiburijima, Oki Islands, Japan
Authors: Momotomi Fuzuki , Raju Aedla , Wang Dongxing , Alsaadi Doaa H. M. , Watanabe Takashi
Published: November 2022

Cardiocrinum cordatum, known as ubayuri in Japan, has antihypertensive properties and has been shown to inhibit angiotensin-converting enzyme (ACE), which contributes to the production of angiotensin II, a hypotensive substance in the renin–angiotensin system. C. cordatum has been the subject of various studies as a useful plant and is applied as a functional food. Due to the limited distribution, loss of natural habitat by frequent natural disasters, and environmental conditions, the chemical content and biological activity of C. cordatum have been drastically affected. Obtaining a stable supply of Cardiocrinu cordatum material with high biological activity is still a challenge. Understanding the native habitat environment and suitable cultivation sites could help in solving this issue. Therefore, in the current study we investigated the effect of environmental parameters on the hypertensive and antioxidant activities of C. cordatum collected at Chiburijima, Oki Islands, Shimane Prefecture, Japan. We also predicted the habitat suitability of C. cordatum using a geographic information system (GIS) and MaxEnt model with various conditioning factors, including the topographic, soil, environmental, and climatic factors of the study area. A total of 37 individual plant samples along with soil data were collected for this study. In vitro assays of ACE inhibitory and antioxidant activity were conducted on the collected samples. The results show that plants at 14 out of 37 sites had very strong ACE inhibitory activity (IC50 < 1 mg mL−1). However, the collected plants showed no signs of strong antioxidant activity. Statistical analysis using analysis of variance (ANOVA) showed that BIO05 (F value = 2.93, p < 0.05), nitrate–nitrogen (F value = 2.46, p < 0.05), and silt (F value = 3.443, p < 0.05) significantly affected ACE inhibitory activity. On the other hand, organic carbon content (F value = 10.986, p < 0.01) was found to significantly affect antioxidant activity. The final habitat suitability map shows 3.3% very high and 6.8% high suitability regions, and samples with ACE inhibition activity were located within these regions. It is recommended further investigations and studies are conducted on C. cordatum in these locations. The prediction suitability model showed accuracy with AUC-ROC of 96.7% for the study area.
Influence of Degree of Polymerization of Low-Molecular-Weight Chitosan Oligosaccharides on the α-Glucosidase Inhibition
Authors: Khaisaat Supharada , Chancharoensin Saovanee , Wipatanawin Angkana , Suphantharika Manop , Payongsri Panwajee
Published: November 2022

Chitosan oligosaccharide (COS) is a bioactive compound derived from marine by-products. COS consumption has been demonstrated to lower the risk of diabetes. However, there are limited data on the inhibitory effect of low-molecular-weight COSs with different degrees of polymerization (DP) on α-glucosidase. This study investigates the α-glucosidase inhibitory activity of two low-molecular-weight COSs, i.e., S-TU-COS with DP2–4 and L-TU-COS with DP2–5, both of which have different molecular weight distributions. The inhibition constants of the inhibitors binding to free enzymes (Ki) and an enzyme–substrate complex (Kii) were investigated to elucidate the inhibitory mechanism of COSs with different chain lengths. The kinetic inhibition model of S-TU-COS showed non-completive inhibition results which are close to the uncompetitive inhibition results with Ki and Kii values of 3.34 mM and 2.94 mM, respectively. In contrast, L-TU-COS showed uncompetitive inhibition with a Kii value of 5.84 mM. With this behavior, the IC50 values of S-TU-COS and L-TU-COS decreased from 12.54 to 11.84 mM and 20.42 to 17.75 mM, respectively, with an increasing substrate concentration from 0.075 to 0.3 mM. This suggests that S-TU-COS is a more potent inhibitor, and the different DP of COS may cause significantly different inhibition (p < 0.05) on the α-glucosidase activity. This research may provide new insights into the production of a COS with a suitable profile for antidiabetic activity.
Silver(I) Complexes Based on Oxadiazole-Functionalized α-Aminophosphonate: Synthesis, Structural Study, and Biological Activities
Authors: Hkiri Shaima , Coşkun Kübra Açıkalın , Üstün Elvan , Samarat Ali , Tutar Yusuf , Şahin Neslihan , Sémeril David
Published: November 2022

Two silver(I) complexes, bis{diethyl[(5-phenyl-1,3,4-oxadiazol-2-yl-κN3:κN4-amino) (4-trifluoromethylphenyl)methyl]phosphonate-(tetrafluoroborato-κF)}-di-silver(I) and tetrakis-{diethyl[(5-phenyl-1,3,4-oxadiazol-2-yl-κN3-amino)(4-trifluoromethylphenyl)methyl]phosphonate} silver(I) tetrafluoroborate, were prepared starting from the diethyl[(5-phenyl-1,3,4-oxadiazol-2-yl-amino)(4-trifluoromethylphenyl)methyl]phosphonate (1) ligand and AgBF4 salt in Ag/ligand ratios of 1/1 and 1/4, respectively. The structure, stoichiometry, and geometry of the silver complexes were fully characterized by elemental analyses, infrared, single-crystal X-ray diffraction studies, multinuclear NMR, and mass spectroscopies. The binuclear complex ([Ag2(1)2(BF4)2]; 2) crystallizes in the monoclinic asymmetric space group P21/c and contains two silver atoms adopting a {AgN2F} planar trigonal geometry, which are simultaneously bridged by two oxadiazole rings of two ligands, while the mononuclear complex ([Ag(1)4]BF4; 3) crystallizes in the non-usual cubic space group Fd-3c in which the silver atom binds to four distinct electronically enriched nitrogen atoms of the oxadiazole ring, in a slightly distorted {AgN4} tetrahedral geometry. The α-aminophosphonate and the monomeric silver complex were evaluated in vitro against MCF-7 and PANC-1 cell lines. The silver complex is promising as a drug candidate for breast cancer and the pancreatic duct with half-maximal inhibitory concentration (IC50) values of 8.3 ± 1.0 and 14.4 ± 0.6 μM, respectively. Additionally, the interactions of the ligand and the mononuclear complex with Vascular Endothelial Growth Factor Receptor-2 and DNA were evaluated by molecular docking methods.
Preparation of PMMA Electrospun Fibers Bearing Porphyrin Pendants and Photocatalytic Degradation of Organic Dyes
Authors: Sun Er-Jun , Bai Xiao-Yan , Chang Yu , Li Qin , Hui Xin-Ru , Li Yan-Song , Wang Yue
Published: November 2022

Porphyrins have a large π–π conjugation force between molecules, and they are easy to aggregate in solution, which affects the photoelectric properties of porphyrins. Connecting porphyrins to polymer links through covalent bonds not only retains the mechanical properties and thermal stability of polymer materials, but also has the photoelectric properties and catalytic properties of porphyrins, which improves the availability of materials. In this study, first, a porphyrin ligand with double bonds in the side chain was designed and the corresponding copper and zinc complexes were synthesized by adjusting the metal ions in the center of the pyrrole ring. Then, the metalloporphyrin complexes were copolymerized with methyl methacrylate (MMA), and two metalloporphyrin/PMMA copolymers were obtained: CPTPPCu/PMMA and CPTPPZn/PMMA. The structure of the compounds was characterized by IR, 1H NMR, MS, and UV-Vis spectra. Metalloporphyrin/PMMA copolymers were prepared into electrospun fiber materials by electrospinning. The morphology of the composites was studied by SEM, and the thermal stability and optical properties of electrospun fibers were studied by TGA and FL. The catalytic activity of electrospun fiber materials for the degradation of organic dyes was studied. The results showed that the efficiency of the metalloporphyrin/PMMA copolymer in photocatalytic degradation of methylene blue (MB) was better than that of the PMMA electrospun fiber blended with metalloporphyrin.
Xanthanolides in Xanthium L.: Structures, Synthesis and Bioactivity
Authors: Zhang Jiaojiao , Zhao Rongmei , Jin Lu , Pan Le , Lei Dongyu
Published: November 2022

Xanthanolides were particularly characteristic of the genus Xanthium, which exhibited broad biological effects and have drawn much attention in pharmacological application. The review surveyed the structures and bioactivities of the xanthanolides in the genus Xanthium, and summarized the synthesis tactics of xanthanolides. The results indicated that over 30 naturally occurring xanthanolides have been isolated from the genus Xanthium in monomeric, dimeric and trimeric forms. The bioassay-guided fractionation studies suggested that the effective fractions on antitumor activities were mostly from weak polar solvents, and xanthatin (1) was the most effective and well-studied xanthanolide. The varieties of structures and structure-activity relationships of the xanthanolides had provided the promising skeleton for the further study. The review aimed at providing guidance for the efficient preparation and the potential prospects of the xanthanolides in the medicinal industry.
Syringaresinol Alleviates Oxaliplatin-Induced Neuropathic Pain Symptoms by Inhibiting the Inflammatory Responses of Spinal Microglia
Authors: Lee Ji Hwan , Choi Jong Hee , Kim Jaihwan , Kim Tai Wan , Kim Ji-Young , Chung Geehoon , Cho Ik-Hyun , Jang Dae Sik , Kim Sun Kwang
Published: November 2022

Oxaliplatin-induced peripheral neuropathy (OIPN) is a serious side effect that impairs the quality of life of patients treated with the chemotherapeutic agent, oxaliplatin. The underlying pathophysiology of OIPN remains unclear, and there are no effective therapeutics. This study aimed to investigate the causal relationship between spinal microglial activation and OIPN and explore the analgesic effects of syringaresinol, a phytochemical from the bark of Cinnamomum cassia, on OIPN symptoms. The causality between microglial activation and OIPN was investigated by assessing cold and mechanical allodynia in mice after intrathecal injection of the serum supernatant from a BV-2 microglial cell line treated with oxaliplatin. The microglial inflammatory response was measured based on inducible nitric oxide synthase (iNOS), phosphorylated extracellular signal-regulated kinase (p-ERK), and phosphorylated nuclear factor-kappa B (p-NF-κB) expression in the spinal dorsal horn. The effects of syringaresinol were tested using behavioral and immunohistochemical assays. We found that oxaliplatin treatment activated the microglia to increase inflammatory responses, leading to the induction of pain. Syringaresinol treatment significantly ameliorated oxaliplatin-induced pain and suppressed microglial expression of inflammatory signaling molecules. Thus, we concluded that the analgesic effects of syringaresinol on OIPN were achieved via the modulation of spinal microglial inflammatory responses.
The Irish Penitentials and Conscience Formation
Authors: Connolly Hugh
Published: November 2022

As commonly used in its moral sense I will, for the purposes of this paper, take the concept of “conscience” to be the inherent ability of every healthy human being to perceive what is right and what is wrong and, on the strength of this perception, to control, monitor, evaluate and execute their actions. Such values as right or wrong, good or evil, just or unjust, and fair or unfair have existed throughout human history and are also shaped by an individual’s cultural, political and economic environment. The medieval penitential literature offers just one such historical snapshot. These manuals or guides for confessors, including prayers, lists of questions to be asked by the confessor, and penances to be assigned for various sins were an integral part of the practice of private penance which began in the Celtic Church and later spread through Europe with the Celtic and Anglo-Saxon missions. Penitential books for use by confessors in private penance appeared in the sixth through ninth centuries. These texts were not as a rule decreed by episcopal synods. Their authority generally rested on the reputation of their compiler or editor. Public penances were assigned for public sins that caused scandal for the church. Private penances were assigned for private sins or matters of conscience. The Penitentials were generally more flexible than the churches’ ancient canonical penitential system which they largely replaced. While later European Penitentials tended to provide more complete guidance for the confessor instead of mere lists of rules and penalties; such instruction is not entirely absent from the earlier Irish texts and ancillary documents. Thus, the goal of penance in the early middle ages was not only sacramental but also didactic. It would have been an occasion to inculcate Christian beliefs, an opportunity to model proper Christian behaviour and by extension a key part of the formation of conscience. As was the case with later expressions of casuistry (Etym. Latin casus, case, or problem to be solved) the purpose of the penitential literature was thus to adapt and apply the unchangeable norms of Christian morality to the changing and variable circumstances of human life albeit in somewhat rudimentary fashion. As such this literary genre and the pastoral practices stemming from it are a valid and worthy object of any historical study and theological analysis concerning the ‘formation of conscience.’
A Review on Machine Learning Applications for Solar Plants
Authors: Engel Ekaterina , Engel Nikita
Published: November 2022

A solar plant system has complex nonlinear dynamics with uncertainties due to variations in system parameters and insolation. Thereby, it is difficult to approximate these complex dynamics with conventional algorithms whereas Machine Learning (ML) methods yield the essential performance required. ML models are key units in recent sensor systems for solar plant design, forecasting, maintenance, and control to provide the best safety, reliability, robustness, and performance as compared to classical methods which are usually employed in the hardware and software of solar plants. Considering this, the goal of our paper is to explore and analyze ML technologies and their advantages and shortcomings as compared to classical methods for the design, forecasting, maintenance, and control of solar plants. In contrast with other review articles, our research briefly summarizes our intelligent, self-adaptive models for sizing, forecasting, maintenance, and control of a solar plant; sets benchmarks for performance comparison of the reviewed ML models for a solar plant’s system; proposes a simple but effective integration scheme of an ML sensor solar plant system’s implementation and outlines its future digital transformation into a smart solar plant based on the integrated cutting-edge technologies; and estimates the impact of ML technologies based on the proposed scheme on a solar plant value chain.
A Wireless Real-Time Continuous Monitoring System for the Internal Movements of Mountain Glaciers Using Sensor Networks
Authors: Wang Shimeng , Xie Aihong , Zhu Jiangping
Published: November 2022

With the escalation of global warming, the shrinkage of mountain glaciers has accelerated globally, the water volume from glaciers has changed, and relative disasters have increased in intensity and frequency (for example, ice avalanches, surging glaciers, and glacial lake outburst floods). However, the wireless monitoring of glacial movements cannot currently achieve omnidirectional, high-precision, real-time results, since there are some technical bottlenecks. Based on wireless networks and sensor application technologies, this study designed a wireless monitoring system for measuring the internal parameters of mountain glaciers, such as temperature, pressure, humidity, and power voltage, and for wirelessly transmitting real-time measurement data. The system consists of two parts, with a glacier internal monitoring unit as one part and a glacier surface base station as the second part. The former wirelessly transmits the monitoring data to the latter, and the latter processes the received data and then uploads the data to a cloud data platform via 4G or satellite signals. The wireless system can avoid cable constraints and transmission failures due to breaking cables. The system can provide more accurate field-monitoring data for simulating glacier movements and further offers an early warning system for glacial disasters.
Developing an Improved Ensemble Learning Approach for Predictive Maintenance in the Textile Manufacturing Process
Authors: Hung Yu-Hsin
Published: November 2022

With the rapid development of digital transformation, paper forms are digitalized as electronic forms (e-Forms). Existing data can be applied in predictive maintenance (PdM) for the enabling of intelligentization and automation manufacturing. This study aims to enhance the utilization of collected e-Form data though machine learning approaches and cloud computing to predict and provide maintenance actions. The ensemble learning approach (ELA) requires less computation time and has a simple hardware requirement; it is suitable for processing e-form data with specific attributes. This study proposed an improved ELA to predict the defective class of product data from a manufacturing site’s work order form. This study proposed the resource dispatching approach to arrange data with the corresponding emailing resource for automatic notification. This study’s novelty is the integration of cloud computing and an improved ELA for PdM to assist the textile product manufacturing process. The data analytics results show that the improved ensemble learning algorithm has over 98% accuracy and precision for defective product prediction. The validation results of the dispatching approach show that data can be correctly transmitted in a timely manner to the corresponding resource, along with a notification being sent to users.
Research on Aerial Autonomous Docking and Landing Technology of Dual Multi-Rotor UAV
Authors: Wang Liang , Jiang Xiangqian , Wang Di , Wang Lisheng , Tu Zhijun , Ai Jianliang
Published: November 2022

This paper studies the cooperative control of multiple unmanned aerial vehicles (UAVs) with sensors and autonomous flight capabilities. In this paper, an architecture is proposed that takes a small quadrotor as a mission UAV and a large six-rotor as a platform UAV to provide an aerial take-off and landing platform and transport carrier for the mission UAV. The design of a tracking controller for an autonomous docking and landing trajectory system is the focus of this research. To examine the system’s overall design, a dual-machine trajectory-tracking control simulation platform is created via MATLAB/Simulink. Then, an autonomous docking and landing trajectory-tracking controller based on radial basis function proportional–integral–derivative control is designed, which fulfills the trajectory-tracking control requirements of the autonomous docking and landing process by efficiently suppressing the external airflow disturbance according to the simulation results. A YOLOv3-based vision pilot system is designed to calibrate the rate of the aerial docking and landing position to eight frames per second. The feasibility of the multi-rotor aerial autonomous docking and landing technology is verified using prototype flight tests during the day and at night. It lays a technical foundation for UAV transportation, autonomous take-off, landing in the air, and collaborative networking. In addition, compared with the existing technologies, our research completes the closed loop of the technical process through modeling, algorithm design and testing, virtual simulation verification, prototype manufacturing, and flight test, which have better realizability.
Nano-Optomechanical Resonators Based on Suspended Graphene for Thermal Stress Sensing
Authors: Liu Shen , Xiao Hang , Chen Yanping , Chen Peijing , Yan Wenqi , Lin Qiao , Liu Bonan , Xu Xizhen , Wang Yiping , Weng Xiaoyu , Liu Liwei , Qu Junle
Published: November 2022

Nanomechanical resonators made from suspended graphene combine the properties of ultracompactness and ultrahigh detection sensitivity, making them interesting devices for sensing applications. However, nanomechanical systems can be affected by membrane stress. The present work developed an optomechanical resonator for thermal stress sensing. The proposed resonator consists of a section of hollow core fiber (HCF) and a trampoline graphene–Au membrane. An all-optical system that integrated optical excitation and optical detection was applied. Then, the resonance frequency of the resonator was obtained through this all-optical system. In addition, this system and the resonator were used to detect the membrane’s built-in stress, which depended on the ambient temperature, by monitoring the resonance frequency shift. The results verified that the temperature-induced thermal effect had a significant impact on membrane stress. Temperature sensitivities of 2.2646 kHz/°C and 2.3212 kHz/°C were obtained when the temperature rose and fell, respectively. As such, we believe that this device will be beneficial for the quality monitoring of graphene mechanical resonators.
Quantifiable Effect of Interparticle Plasmonic Coupling on Sensitivity and Tuning Range for Wavelength-Mode LSPR Fiber Sensor Fabricated by Simple Immobilization Method
Authors: Jia Shuo , Ma Aiwen , Dong Hanpeng , Xia Shanhong
Published: November 2022

Herein a gold nanosphere (AuNS)-coated wavelength-mode localized surface plasmon resonance (LSPR) fiber sensor was fabricated by a simple and time-saving electrostatic self-assembly method using poly(allylamine hydrochloride). Based on the localized enhanced coupling effect between AuNSs, the LSPR spectrums of the AuNS monolayer with good dispersity and high density exhibited a favourable capability for refractive index (RI) measurement. Based on the results obtained from the optimization for AuNS distribution, sensing length, and RI range, the best RI sensitivity of the fiber modified by 100 nm AuNS reached up to about 2975 nm/RIU, with the surrounding RI range from 1.3322 to 1.3664. Using an 80 nm AuNS-modified fiber sensor, the RI sensitivity of 3953 nm/RIU was achieved, with the RI range increased from 1.3744 to 1.3911. The effect of sensing length to RI sensitivity was proven to be negligible. Furthermore, the linear relationship between the RI sensitivity and plasma resonance frequency of the bulk metal, which was dependent on the interparticle plasmon coupling effect, was quantified. Additionally, the resonance peak was tuned from 539.18 nm to 820.48 nm by different sizes of AuNSs-coated fiber sensors at a RI of 1.3322, which means the spectrum was extended from VIS to NIR. It has enormous potential in hypersensitive biochemistry detection at VIS and NIR ranges.
IoT Clusters for Enhancing Multimedia Applications
Authors: Coelho Jorge , Nogueira Luís
Published: November 2022

In this paper, we present a framework for exploring the spare capacity of IoT devices for clustered execution of multimedia applications. Applications of this type are usually framed with specific quality parameters that enable a desirable level of service. This means that the IoT cluster must guarantee strict quality ranges of service to work as expected. The framework is totally customizable, and QoS dimensions can be easily added or removed given their relevance in the application scenario. The achieved results clearly demonstrate the utility of using the spare capacity of IoT devices, otherwise unused, to cooperatively execute servies within the desired quality of service levels.
Classification Models of Action Research Arm Test Activities in Post-Stroke Patients Based on Human Hand Motion
Authors: Padilla-Magaña Jesus Fernando , Peña-Pitarch Esteban
Published: November 2022

The Action Research Arm Test (ARAT) presents a ceiling effect that prevents the detection of improvements produced with rehabilitation treatments in stroke patients with mild finger joint impairments. The aim of this study was to develop classification models to predict whether activities with similar ARAT scores were performed by a healthy subject or by a subject post-stroke using the extension and flexion angles of 11 finger joints as features. For this purpose, we used three algorithms: Support Vector Machine (SVM), Random Forest (RF), and K-Nearest Neighbors (KNN). The dataset presented class imbalance, and the classification models presented a low recall, especially in the stroke class. Therefore, we implemented class balance using Borderline-SMOTE. After data balancing the classification models showed significantly higher accuracy, recall, f1-score, and AUC. However, after data balancing, the SVM classifier showed a higher performance with a precision of 98%, a recall of 97.5%, and an AUC of 0.996. The results showed that classification models based on human hand motion features in combination with the oversampling algorithm Borderline-SMOTE achieve higher performance. Furthermore, our study suggests that there are differences in ARAT activities performed between healthy and post-stroke individuals that are not detected by the ARAT scoring process.
Influence of Insufficient Dataset Augmentation on IoU and Detection Threshold in CNN Training for Object Detection on Aerial Images
Authors: Bożko Arkadiusz , Ambroziak Leszek
Published: November 2022

The objects and events detection tasks are being performed progressively often by robotic systems like unmanned aerial vehicles (UAV) or unmanned surface vehicles (USV). Autonomous operations and intelligent sensing are becoming standard in numerous scenarios such as supervision or even search and rescue (SAR) missions. The low cost of autonomous vehicles, vision sensors and portable computers allows the incorporation of the deep learning, mainly convolutional neural networks (CNN) in these solutions. Many systems meant for custom purposes rely on insufficient training datasets, what may cause a decrease of effectiveness. Moreover, the system’s accuracy is usually dependent on the returned bounding boxes highlighting the supposed targets. In desktop applications, precise localisation might not be particularly relevant; however, in real situations, with low visibility and non-optimal camera orientation, it becomes crucial. One of the solutions for dataset enhancement is its augmentation. The presented work is an attempt to evaluate the influence of the training images augmentation on the detection parameters important for the effectiveness of neural networks in the context of object detection. In this research, network appraisal relies on the detection confidence and bounding box prediction accuracy (IoU). All the applied image modifications were simple pattern and colour alterations. The obtained results imply that there is a measurable impact of the augmentation process on the localisation accuracy. It was concluded that a positive or negative influence is related to the complexity and variability of the objects classes.
The existence of man and the world in the context of their alienness to each other
Authors:   Financial University under the Government of the Russian Federation - Kursk branch of the Financial University, Panishchev A.L. , Shcherbakov D.B. ,   Kursk Branch of the Financial University under the Government of the Russian Federation
Published: November 2022

This article is devoted to the peculiarities of human development in a world that is ontologically distinct from it. At the same time, people, as their civilizational development moves further and further away from the natural environment and realize their existence in conditions that are artificially created. The most vivid form of human existence, distinguishable from the natural environment, has become the state. It is in it that a person builds such a system of relations with the world and other people in which he is able to realize his qualities in the most comprehensive way. A number of inventions that alienated people from nature were made in the conditions of state life of society. Moreover, in recent years, there has been a tendency for such conditions to be virtual, which is associated with the development of information technologies. In an ethical sense, it is not always possible to give an unambiguous answer to characterize the phenomena occurring, but the human community must respond to all these phenomena and adapt to new conditions. Nevertheless, a humanitarian understanding of the ongoing processes is important, and their correction will probably be necessary in order to preserve not only the basic qualities of a person that distinguish him from nature, but also to reduce various kinds of risks, including environmental ones.