bluuebunny/crossref_metadata_2025 huggingface dataset:
Helicobacter pylori and oral–gut microbiome: clinical implications
Authors: Elghannam Maged T. , Hassanien Moataz H. , Ameen Yosry A. , Turky Emad A. , ELattar Gamal M. , ELRay Ahmed A. , ELTalkawy Mohammed D.
Published: November 2023

More than half of the world’s population are colonized with H. pylori; however, the prevalence varies geographically with the highest incidence in Africa. H. pylori is probably a commensal organism that has been associated with the development of gastritis, ulcers, and gastric cancer. H. pylori alone is most probably not enough for the development of gastric carcinoma, but evidence for its association with the disease is high and has, therefore, been classified by the International Agency for Research on Cancer as a Class 1 carcinogen. Bacteroidetes and Fusobacteria positively coexisted during H. pylori infection along the oral–gut axis. The eradication therapy required to treat H. pylori infection can also have detrimental consequences for the gut microbiota, leading to a decreased alpha diversity. Therefore, therapy regimens integrated with probiotics may abolish the negative effects of antibiotic therapy on the gut microbiota. These eradication therapies combined with probiotics have also higher rates of eradication, when compared to standard treatments, and are associated with reduced side effects, improving the patient’s compliance. The eradication therapy not only affects gut microbiome but also affects the oral microbiome with robust predominance of harmful bacteria. However, there have been reports of a protective role of H. pylori in Barrett’s esophagus, esophageal adenocarcinoma, eosinophilic esophagitis, IBD, asthma, and even multiple sclerosis. Therefore, eradication therapy should be carefully considered, and test to treat policy should be tailored to specific communities especially in highly endemic areas. Supplementation of probiotics, prebiotics, herbals, and microbial metabolites to reduce the negative effects of eradication therapy should be considered. After failure of many eradication attempts, the benefits of H. pylori eradication should be carefully balanced against the risk of adverse effects especially in the elderly, persons with frailty, and intolerance to antibiotics.
Measuring implicit associations between food and body stimuli in anorexia nervosa: a Go/No-Go Association Task
Authors: Lakritz Clara , Iceta Sylvain , Duriez Philibert , Makdassi Maxime , Masetti Vincent , Davidenko Olga , Lafraire Jérémie
Published: November 2023

Purpose

The present study aimed to explore the implicit associations between food and bodily stimuli in patients with anorexia nervosa (AN) and control subjects (HC).

Methods

A Go/No-Go Association Task was administrated to 55 participants (28 AN and 27 HC), using food stimuli (low-calorie food vs. high-calorie food) and body stimuli (underweight vs. overweight bodies).

Results

We evidenced an implicit association between food and body stimuli in the AN group, whereas the HC group only showed a tendency. AN and HC groups also exhibited different categorization strategies: the AN group tended to categorize stimuli as low-calorie foods and underweight bodies less than the HC group, and they tended to categorize stimuli as high-calorie foods and overweight bodies more than the HC group.

Conclusion

The present study revealed for the first time specificities of the AN population’s implicit association between food and body stimuli in terms of association strength and categorization strategy. Furthermore, the results suggest that combining implicit methodologies with other methods could contribute to a better characterization of the physiopathology of AN.

Level of evidence

Level I, experimental study.
Diverse Structural Design Strategies of MXene-Based Macrostructure for High-Performance Electromagnetic Interference Shielding
Authors: Liu Yue , Wang Yadi , Wu Na , Han Mingrui , Liu Wei , Liu Jiurong , Zeng Zhihui
Published: November 2023

There is an urgent demand for flexible, lightweight, mechanically robust, excellent electromagnetic interference (EMI) shielding materials. Two-dimensional (2D) transition metal carbides/nitrides (MXenes) have been potential candidates for the construction of excellent EMI shielding materials due to their great electrical electroconductibility, favorable mechanical nature such as flexibility, large aspect ratios, and simple processability in aqueous media. The applicability of MXenes for EMI shielding has been intensively explored; thus, reviewing the relevant research is beneficial for advancing the design of high-performance MXene-based EMI shields. Herein, recent progress in MXene-based macrostructure development is reviewed, including the associated EMI shielding mechanisms. In particular, various structural design strategies for MXene-based EMI shielding materials are highlighted and explored. In the end, the difficulties and views for the future growth of MXene-based EMI shields are proposed. This review aims to drive the growth of high-performance MXene-based EMI shielding macrostructures on basis of rational structural design and the future high-efficiency utilization of MXene.
Green-Solvent Processed Blade-Coating Organic Solar Cells with an Efficiency Approaching 19% Enabled by Alkyl-Tailored Acceptors
Authors: Bai Hairui , Ma Ruijie , Su Wenyan , Peña Top Archie Dela , Li Tengfei , Tang Lingxiao , Yang Jie , Hu Bin , Wang Yilin , Bi Zhaozhao , Su Yueling , Wei Qi , Wu Qiang , Duan Yuwei , Li Yuxiang , Wu Jiaying , Ding Zicheng , Liao Xunfan , Huang Yinjuan , Gao Chao , Lu Guanghao , Li Mingjie , Zhu Weiguo , Li Gang , Fan Qunping , Ma Wei
Published: November 2023

Power-conversion-efficiencies (PCEs) of organic solar cells (OSCs) in laboratory, normally processed by spin-coating technology with toxic halogenated solvents, have reached over 19%. However, there is usually a marked PCE drop when the blade-coating and/or green-solvents toward large-scale printing are used instead, which hampers the practical development of OSCs. Here, a new series of N-alkyl-tailored small molecule acceptors named YR-SeNF with a same molecular main backbone are developed by combining selenium-fused central-core and naphthalene-fused end-group. Thanks to the N-alkyl engineering, NIR-absorbing YR-SeNF series show different crystallinity, packing patterns, and miscibility with polymeric donor. The studies exhibit that the molecular packing, crystallinity, and vertical distribution of active layer morphologies are well optimized by introducing newly designed guest acceptor associated with tailored N-alkyl chains, providing the improved charge transfer dynamics and stability for the PM6:L8-BO:YR-SeNF-based OSCs. As a result, a record-high PCE approaching 19% is achieved in the blade-coating OSCs fabricated from a green-solvent o-xylene with high-boiling point. Notably, ternary OSCs offer robust operating stability under maximum-power-point tracking and well-keep > 80% of the initial PCEs for even over 400 h. Our alkyl-tailored guest acceptor strategy provides a unique approach to develop green-solvent and blade-coating processed high-efficiency and operating stable OSCs, which paves a way for industrial development.
Optimal Mass Transport on Euclidean Spaces
Authors: Maggi Francesco
Published: November 2023

Optimal mass transport has emerged in the past three decades as an active field with wide-ranging connections to the calculus of variations, PDEs, and geometric analysis. This graduate-level introduction covers the field's theoretical foundation and key ideas in applications. By focusing on optimal mass transport problems in a Euclidean setting, the book is able to introduce concepts in a gradual, accessible way with minimal prerequisites, while remaining technically and conceptually complete. Working in a familiar context will help readers build geometric intuition quickly and give them a strong foundation in the subject. This book explores the relation between the Monge and Kantorovich transport problems, solving the former for both the linear transport cost (which is important in geometric applications) and for the quadratic transport cost (which is central in PDE applications), starting from the solution of the latter for arbitrary transport costs.
African Military Politics in the Sahel
Authors: Döring Katharina P. W.
Published: November 2023

Challenging western and francocentric accounts of military interventions in the Sahel, Katharina P. W. Döring foregrounds the response of African regional organizations to armed violence since 2012. Based on extensive empirical research, she reconstructs the experiences of African intervenors in planning and deploying missions in the region. The book outlines the complex constellation of actors who shape African military politics, including presidents, diplomats, and bureaucrats. Drawing upon insights from critical geography, Döring considers the oft-neglected role that space – at once relational and changing – plays in the power dynamics of the region. In so doing, she offers a fresh perspective on military deployments and their politics. Amidst the current resurgence of nationalist geopolitics, this study and its ﬁndings have far-reaching implications for the analysis of military politics in Africa and beyond.
Nietzsche's Struggle against Pessimism
Authors: Hassan Patrick
Published: November 2023

On what grounds could life be made worth living, given its abundant suffering? Friedrich Nietzsche was among many who attempted to answer this question. While always seeking to resist pessimism, Nietzsche's strategy for doing so, and the extent to which he was willing to concede conceptual grounds to pessimists, shifted dramatically over time. His reading of pessimists such as Eduard von Hartmann, Olga Plümacher, and Julius Bahnsen—as well as their critics, such as Eugen Dühring and James Sully—has been under-explored in the secondary literature, isolating him from his intellectual context. Patrick Hassan's book seeks to correct this. After closely mapping Nietzsche's philosophical development on to the relevant axiological and epistemological issues, it disentangles his various critiques of pessimism, elucidating how familiar Nietzschean themes (e.g. eternal recurrence, aesthetic justification, will to power, and his critique of Christianity) can and should be assessed against this philosophical backdrop.
Long‐Term Variability in the Arabian Peninsula Droughts Driven by the Atlantic Multidecadal Oscillation
Authors: Saharwardi Md Saquib , Dasari Hari Prasad , Aggarwal Vaneet , Ashok Karumuri , Hoteit Ibrahim
Published: November 2023

Drought is a recurring hydroclimatic extreme over the Arabian Peninsula (AP). So far, no study has examined the changes in drought characteristics in recent decades, not to mention the background mechanisms for such changes. To this end, analyzing the Standardized Precipitation Evapotranspiration Index (SPEI) mainly from the European Reanalysis (ERA5) data sets, in addition to other observational/reanalysis data sets over the period of 1951–2020, we show that droughts over the AP have increased in frequency and severity over the last two decades. We show that this drought acceleration, which was not observed in the previous 40–50 years, is a combination of decadal variability and long‐term trends. Importantly, we demonstrate that the decadal SPEI variability is due to the Atlantic Multidecadal Oscillation (AMO). The unprecedented multiyear drought over the AP in recent decades is evidently associated with the current positive phase of the AMO. We also show that the recent warming of the AP is a more significant factor in the drought intensification than the concurrent weakening of local precipitation. Furthermore, we developed a machine learning model largely based on the observed AMO–SPEI relationship. This model predicts a reduced drought severity over the AP in the near future.
The Role of Convectively Coupled Equatorial Waves in Sub‐Seasonal Predictions
Authors: Dias Juliana , Gehne Maria , Kiladis George N. , Magnusson Linus
Published: November 2023

Convectively coupled equatorial waves (CCEWs) are modes of tropical variability that are often seen as potentially beneficial for sub‐seasonal predictions. Quantifying this potential has been proven difficult in operational forecast systems because tropical variability tends to be poorly represented in such models. The European Center for Medium‐Range Weather Forecasts (ECMWF) is a potential exception because it has been shown to have much improved representation of the tropics. Here, ECMWF reforecasts are used to investigate the predicted evolution of CCEWs and how they might impact sub‐seasonal predictions. It is shown that the ECMWF system is able to generate variability consistent with various modes of CCEWs, but CCEWs tend to be weaker in amplitude and their propagation characteristics often deviate from observations. This study suggests that model advancements aimed at improving the deterministic representation of CCEWs are still needed in order to better understand and utilize tropical sources of sub‐seasonal predictive skill.
Polar and Equatorial Ionospheric Electrodynamical Coupling Under a Prolonged Northward Bz Interval
Authors: Li Qiaoling , Li Shuhan , Chen Junjie , Liu Jing , Zhang Ruilong , Liu Libo , Kuai Jiawei
Published: November 2023

The interplanetary magnetic field (IMF) significantly influences the global ionospheric electrodynamics, but it is still largely unknown the high‐ and low‐latitude ionospheric electrodynamical coupling under long‐duration northward IMF Bz (NBz) and By conditions. During the long‐duration NBz and duskward By conditions (Kp < 2, AE < 100 nT, SYM‐H > −25 nT) on 20 August 2014, six pairs of magnetometer data showed that daytime equatorial electrojet (EEJ) underwent strong decreases (reach up to 90%) at wide longitudes. Thermosphere‐ionosphere‐electrodynamics general circulation model (TIE‐GCM) captured well the decrease and the control simulations revealed that both effects of the NBz and By significantly change the plasma convection, Joule heating, and thermospheric winds at high latitudes; and penetration electric field (PEF) due to the NBz plays an important role in the decrease of the equatorial electric field. This study indicates the significant NBz effects on the equatorial ionospheric electrodynamics even during weak geomagnetic conditions, apart from the widely believed meteorological effects. Further study should be taken to disclose the relative contribution between geomagnetic and meteorological effects on the decrease of the equatorial electric field.
Fractal photonic anomalous Floquet topological insulators to generate multiple quantum chiral edge states
Authors: Li Meng , Li Chu , Yan Linyu , Li Qiang , Gong Qihuang , Li Yan
Published: November 2023

Anomalous Floquet topological insulators with vanishing Chern numbers but supporting chiral edge modes are attracting more and more attention. Since the existing anomalous Floquet topological insulators usually support only one kind of chiral edge mode even at a large lattice size, they are unscalable and unapplicable for multistate topological quantum systems. Recently, fractal topological insulators with self-similarity have been explored to support more nontrivial modes. Here, we demonstrate the first experimental realization of fractal photonic anomalous Floquet topological insulators based on dual Sierpinski carpet consisting of directional couplers using the femtosecond laser direct writing. The fabricated lattices support much more kinds of chiral edge states with fewer waveguides and enable perfect hopping of quantum states with near unit transfer efficiency. Instead of zero-dimensional bound modes for quantum state transport in previous laser direct-written topological insulators, we generate multiple propagating single-photon chiral edge states in the fractal lattice and observe high-visibility quantum interferences. These suggest the successful realization of highly indistinguishable single-photon chiral edge states, which can be applied in various quantum operations. This work provides the potential for enhancing the multi-fold manipulation of quantum states, enlarging the encodable quantum information capacity in a single lattice via high-dimensional encoding and many other fractal applications.
Experimental realisation of multi-qubit gates using electron paramagnetic resonance
Authors: Little Edmund J. , Mrozek Jacob , Rogers Ciarán J. , Liu Junjie , McInnes Eric J. L. , Bowen Alice M. , Ardavan Arzhang , Winpenny Richard E. P.
Published: November 2023

Quantum information processing promises to revolutionise computing; quantum algorithms have been discovered that address common tasks significantly more efficiently than their classical counterparts. For a physical system to be a viable quantum computer it must be possible to initialise its quantum state, to realise a set of universal quantum logic gates, including at least one multi-qubit gate, and to make measurements of qubit states. Molecular Electron Spin Qubits (MESQs) have been proposed to fulfil these criteria, as their bottom-up synthesis should facilitate tuning properties as desired and the reproducible production of multi-MESQ structures. Here we explore how to perform a two-qubit entangling gate on a multi-MESQ system, and how to readout the state via quantum state tomography. We propose methods of accomplishing both procedures using multifrequency pulse Electron Paramagnetic Resonance (EPR) and apply them to a model MESQ structure consisting of two nitroxide spin centres. Our results confirm the methodological principles and shed light on the experimental hurdles which must be overcome to realise a demonstration of controlled entanglement on this system.
A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer’s disease
Authors: Aron Liviu , Qiu Chenxi , Ngian Zhen Kai , Liang Marianna , Drake Derek , Choi Jaejoon , Fernandez Marty A. , Roche Perle , Bunting Emma L. , Lacey Ella K. , Hamplova Sara E. , Yuan Monlan , Wolfe Michael S. , Bennett David A. , Lee Eunjung A. , Yankner Bruce A.
Published: November 2023

Many aging individuals accumulate the pathology of Alzheimer’s disease (AD) without evidence of cognitive decline. Here we describe an integrated neurodegeneration checkpoint response to early pathological changes that restricts further disease progression and preserves cognitive function. Checkpoint activation is mediated by the REST transcriptional repressor, which is induced in cognitively-intact aging humans and AD mouse models at the onset of amyloid β-protein (Aβ) deposition and tau accumulation. REST induction is mediated by the unfolded protein response together with β-catenin signaling. A consequence of this response is the targeting of REST to genes involved in key pathogenic pathways, resulting in downregulation of gamma secretase, tau kinases, and pro-apoptotic proteins. Deletion of REST in the 3xTg and J20 AD mouse models accelerates Aβ deposition and the accumulation of misfolded and phosphorylated tau, leading to neurodegeneration and cognitive decline. Conversely, viral-mediated overexpression of REST in the hippocampus suppresses Aβ and tau pathology. Thus, REST mediates a neurodegeneration checkpoint response with multiple molecular targets that may protect against the onset of AD.
Intrinsic surface p-wave superconductivity in layered AuSn4
Authors: Zhu Wenliang , Song Rui , Huang Jierui , Wang Qi-Wei , Cao Yuan , Zhai Runqing , Bian Qi , Shao Zhibin , Jing Hongmei , Zhu Lujun , Hou Yuefei , Gao Yu-Hang , Li Shaojian , Zheng Fawei , Zhang Ping , Pan Mojun , Liu Junde , Qu Gexing , Gu Yadong , Zhang Hao , Dong Qinxin , Huang Yifei , Yuan Xiaoxia , He Junbao , Li Gang , Qian Tian , Chen Genfu , Li Shao-Chun , Pan Minghu , Xue Qi-Kun
Published: November 2023

The search for topological superconductivity (TSC) is currently an exciting pursuit, since non-trivial topological superconducting phases could host exotic Majorana modes. However, the difficulty in fabricating proximity-induced TSC heterostructures, the sensitivity to disorder and stringent topological restrictions of intrinsic TSC place serious limitations and formidable challenges on the materials and related applications. Here, we report a new type of intrinsic TSC, namely intrinsic surface topological superconductivity (IS-TSC) and demonstrate it in layered AuSn4 with Tc of 2.4 K. Different in-plane and out-of-plane upper critical fields reflect a two-dimensional (2D) character of superconductivity. The two-fold symmetric angular dependences of both magneto-transport and the zero-bias conductance peak (ZBCP) in point-contact spectroscopy (PCS) in the superconducting regime indicate an unconventional pairing symmetry of AuSn4. The superconducting gap and surface multi-bands with Rashba splitting at the Fermi level (EF), in conjunction with first-principle calculations, strongly suggest that 2D unconventional SC in AuSn4 originates from the mixture of p-wave surface and s-wave bulk contributions, which leads to a two-fold symmetric superconductivity. Our results provide an exciting paradigm to realize TSC via Rashba effect on surface superconducting bands in layered materials.
Efficient ultrafast field-driven spin current generation for spintronic terahertz frequency conversion
Authors: Ilyakov Igor , Brataas Arne , de Oliveira Thales V. A. G. , Ponomaryov Alexey , Deinert Jan-Christoph , Hellwig Olav , Faßbender Jürgen , Lindner Jürgen , Salikhov Ruslan , Kovalev Sergey
Published: November 2023

Efficient generation and control of spin currents launched by terahertz (THz) radiation with subsequent ultrafast spin-to-charge conversion is the current challenge for the next generation of high-speed communication and data processing units. Here, we demonstrate that THz light can efficiently drive coherent angular momentum transfer in nanometer-thick ferromagnet/heavy-metal heterostructures. This process is non-resonant and does neither require external magnetic fields nor cryogenics. The efficiency of this process is more than one order of magnitude higher as compared to the recently observed THz-induced spin pumping in MnF2 antiferromagnet. The coherently driven spin currents originate from the ultrafast spin Seebeck effect, caused by a THz-induced temperature imbalance in electronic and magnonic temperatures and fast relaxation of the electron-phonon system. Owing to the fact that the electron-phonon relaxation time is comparable with the period of a THz wave, the induced spin current results in THz second harmonic generation and THz optical rectification, providing a spintronic basis for THz frequency mixing and rectifying components.
Diamond preservation in the lithospheric mantle recorded by olivine in kimberlites
Authors: Giuliani Andrea , Phillips David , Pearson D. Graham , Sarkar Soumendu , Müller Alex A. , Weiss Yaakov , Preston Robin , Seller Michael , Spetsius Zdislav
Published: November 2023

The diamond potential of kimberlites is difficult to assess due to several mantle and magmatic processes affecting diamond content. Traditionally, initial evaluations are based on the compositions of mantle-derived minerals (garnet, chromite, clinopyroxene), which allow an assessment of pressure-temperature conditions and lithologies suitable for diamond formation. Here we explore a complementary approach that considers the conditions of diamonds destruction by interaction with melts/fluids (metasomatism). We test the hypothesis that carbonate-rich metasomatism related to kimberlite melt infiltration into the deep lithosphere is detrimental to diamond preservation. Our results show that high diamond grades in kimberlites worldwide are exclusively associated with high-Mg/Fe olivine, which corresponds to mantle lithosphere minimally affected by kimberlite-related metasomatism. Diamond dissolution in strongly metasomatised lithosphere containing low-Mg/Fe olivine provides a causal link to the empirical associations between low diamond grades, abundant Ti-Zr-rich garnets and kimberlites with high Ti and low Mg contents. This finding show-cases olivine geochemistry as a viable tool in diamond exploration.
Single-nucleus DNA sequencing reveals hidden somatic loss-of-heterozygosity in Cerebral Cavernous Malformations
Authors: Ressler Andrew K. , Snellings Daniel A. , Girard Romuald , Gallione Carol J. , Lightle Rhonda , Allen Andrew S. , Awad Issam A. , Marchuk Douglas A.
Published: November 2023

Cerebral Cavernous Malformations (CCMs) are vascular malformations of the central nervous system which can lead to moderate to severe neurological phenotypes in patients. A majority of CCM lesions are driven by a cancer-like three-hit mutational mechanism, including a somatic, activating mutation in the oncogene PIK3CA, as well as biallelic loss-of-function mutations in a CCM gene. However, standard sequencing approaches often fail to yield a full complement of pathogenic mutations in many CCMs. We suggest this reality reflects the limited sensitivity to identify low-frequency variants and the presence of mutations undetectable with bulk short-read sequencing. Here we report a single-nucleus DNA-sequencing approach that leverages the underlying biology of CCMs to identify lesions with somatic loss-of-heterozygosity, a class of such hidden mutations. We identify an alternative genetic mechanism for CCM pathogenesis and establish a method that can be repurposed to investigate the genetic underpinning of other disorders with multiple somatic mutations.
An atlas of genetic determinants of forearm fracture
Authors: Nethander Maria , Movérare-Skrtic Sofia , Kämpe Anders , Coward Eivind , Reimann Ene , Grahnemo Louise , Borbély Éva , Helyes Zsuzsanna , Funck-Brentano Thomas , Cohen-Solal Martine , Tuukkanen Juha , Koskela Antti , Wu Jianyao , Li Lei , Lu Tianyuan , Gabrielsen Maiken E. ,   Estonian Biobank Research Team, Mägi Reedik , Hoff Mari , Lerner Ulf H. , Henning Petra , Ullum Henrik , Erikstrup Christian , Brunak Søren ,   DBDS Genomic Consortium, Langhammer Arnulf , Tuomi Tiinamaija , Oddsson Asmundur , Stefansson Kari , Pettersson-Kymmer Ulrika , Ostrowski Sisse Rye , Pedersen Ole Birger Vesterager , Styrkarsdottir Unnur , Mäkitie Outi , Hveem Kristian , Richards J. Brent , Ohlsson Claes
Published: November 2023

Osteoporotic fracture is among the most common and costly of diseases. While reasonably heritable, its genetic determinants have remained elusive. Forearm fractures are the most common clinically recognized osteoporotic fractures with a relatively high heritability. To establish an atlas of the genetic determinants of forearm fractures, we performed genome-wide association analyses including 100,026 forearm fracture cases. We identified 43 loci, including 26 new fracture loci. Although most fracture loci associated with bone mineral density, we also identified loci that primarily regulate bone quality parameters. Functional studies of one such locus, at TAC4, revealed that Tac4–/– mice have reduced mechanical bone strength. The strongest forearm fracture signal, at WNT16, displayed remarkable bone-site-specificity with no association with hip fractures. Tall stature and low body mass index were identified as new causal risk factors for fractures. The insights from this atlas may improve fracture prediction and enable therapeutic development to prevent fractures.
Cell-based versus corticosteroid injections for knee pain in osteoarthritis: a randomized phase 3 trial
Authors: Mautner Ken , Gottschalk Michael , Boden Scott D. , Akard Alison , Bae Won C. , Black Lora , Boggess Blake , Chatterjee Paramita , Chung Christine B. , Easley Kirk A. , Gibson Greg , Hackel Josh , Jensen Katie , Kippner Linda , Kurtenbach Chad , Kurtzberg Joanne , Mason R. Amadeus , Noonan Benjamin , Roy Krishnendu , Valentine Verle , Yeago Carolyn , Drissi Hicham
Published: November 2023

Various types of cellular injection have become a popular and costly treatment option for patients with knee osteoarthritis despite a paucity of literature establishing relative efficacy to each other or corticosteroid injections. Here we aimed to identify the safety and efficacy of cell injections from autologous bone marrow aspirate concentrate, autologous adipose stromal vascular fraction and allogeneic human umbilical cord tissue-derived mesenchymal stromal cells, in comparison to corticosteroid injection (CSI). The study was a phase 2/3, four-arm parallel, multicenter, single-blind, randomized, controlled clinical trial with 480 patients with a diagnosis of knee osteoarthritis (Kellgren–Lawrence II–IV). Participants were randomized to the three different arms with a 3:1 distribution. Arm 1: autologous bone marrow aspirate concentrate (n = 120), CSI (n = 40); arm 2: umbilical cord tissue-derived mesenchymal stromal cells (n = 120), CSI (n = 40); arm 3: stromal vascular fraction (n = 120), CSI (n = 40). The co-primary endpoints were the visual analog scale pain score and Knee injury and Osteoarthritis Outcome Score pain score at 12 months versus baseline. Analyses of our primary endpoints, with 440 patients, revealed that at 1 year post injection, none of the three orthobiologic injections was superior to another, or to the CSI control. In addition, none of the four groups showed a significant change in magnetic resonance imaging osteoarthritis score compared to baseline. No procedure-related serious adverse events were reported during the study period. In summary, this study shows that at 1 year post injection, there was no superior orthobiologic as compared to CSI for knee osteoarthritis. ClinicalTrials.gov Identifier: NCT03818737
Quantitative study of spatial and temporal variation in retinal capillary network perfusion in rat eye by in vivo confocal imaging
Authors: Yu Paula Kun , Mehnert Andrew , Dickson Jayden Brendan , Qambari Hassanain , Balaratnasingam Chandrakumar , Cringle Stephen , Darcey Dean , Yu Dao-Yi
Published: November 2023

Microvascular dysfunction is the underlying pathological process in many systemic diseases. However, investigation into its pathogenesis is impeded by the accessibility and complexity of the microvasculature within different organs, particularly for the central nervous system. The retina as an extension of the cerebrum provides a glimpse into the brain through which the microvasculature can be observed. Two major questions remain unanswered: How do the microvessels regulate spatial and temporal delivery to satisfy the varying cellular demands, and how can we quantify blood perfusion in the 3D capillary network? Here, quantitative measurements of red blood cell (RBC) speed in each vessel in the field were made in the in vivo rat retinal capillary network using an ultrafast confocal technique with fluorescently labelled RBCs. Retinal RBC speed and number were found to vary remarkably between microvessels ranging from 215 to 6641 microns per second with significant variations spatially and temporally. Overall, the RBC speed was significantly faster in the microvessels in the superficial retina than in the deep retina (estimated marginal means of 2405 ± 238.2 µm/s, 1641 ± 173.0 µm/s respectively). These observations point to a highly dynamic nature of microvasculature that is specific to its immediate cellular environment and is constantly changing.
Treatment of 95 post-Covid patients with SSRIs
Authors: Rus Carla P. , de Vries Bert E. K. , de Vries Ingmar E. J. , Nutma Idelette , Kooij J. J. Sandra
Published: November 2023

After Covid-19 infection, 12.5% develops post-Covid-syndrome (PCS). Symptoms indicate numerous affected organ systems. After a year, chronic fatigue, dysautonomia and neurological and neuropsychiatric complaints predominate. In this study, 95 PCS patients were treated with selective serotonin reuptake inhibitors (SSRIs). This study used an exploratory questionnaire and found that two-thirds of patients had a reasonably good to strong response on SSRIs, over a quarter of patients had moderate response, while 10% reported no response. Overall, patients experienced substantial improved well-being. Brainfog and sensory overload decreased most, followed by chronic fatigue and dysautonomia. Outcomes were measured with three different measures that correlated strongly with each other. The response to SSRIs in PCS conditions was explained by seven possible neurobiological mechanisms based on recent literature on PCS integrated with already existing knowledge. Important for understanding these mechanisms is the underlying biochemical interaction between various neurotransmitter systems and parts of the immune system, and their dysregulation in PCS. The main link appears to be with the metabolic kynurenine pathway (KP) which interacts extensively with the immune system. The KP uses the same precursor as serotonin: tryptophan. The KP is overactive in PCS which maintains inflammation and which causes a lack of tryptophan. Finally, potential avenues for future research to advance this line of clinical research are discussed.
Controlling stable Bloch points with electric currents
Authors: Lang Martin , Pathak Swapneel Amit , Holt Samuel J. R. , Beg Marijan , Fangohr Hans
Published: November 2023

The Bloch point is a point singularity in the magnetisation configuration, where the magnetisation vanishes. It can exist as an equilibrium configuration and plays an important role in many magnetisation reversal processes. In the present work, we focus on manipulating Bloch points in a system that can host stable Bloch points—a two-layer FeGe nanostrip with opposite chirality of the two layers. We drive Bloch points using spin-transfer torques and find that Bloch points can move collectively without any Hall effect and report that Bloch points are repelled from the sample boundaries and each other. We study pinning of Bloch points at wedge-shaped constrictions (notches) in the nanostrip and demonstrate that arrays of Bloch points can be moved past a series of notches in a controlled manner by applying consecutive current pulses of different strength. Finally, we simulate a T-shaped geometry and demonstrate that a Bloch point can be moved along different paths by applying current between suitable strip ends.
Sex-specific-evaluation of metabolic syndrome prevalence in Algeria: insights from the 2016–2017 non-communicable diseases risk factors survey
Authors: Ngwasiri Calypse , Kinoré Mikaila , Samadoulougou Sekou , Kirakoya-Samadoulougou Fati
Published: November 2023

Metabolic syndrome (MetS) is a core driver of cardiovascular diseases (CVD); however, to date, gender differences in MetS prevalence and its components have not been assessed in the Algerian adult general population. This study aimed to determine the gender differences in MetS prevalence and its components, in the general population of Algeria. Secondary analysis was performed on data from the Algerian 2016–2017 non-communicable disease risk factor survey. MetS was determined according to the harmonized Joint Interim Statement criteria. A Poisson regression model based on Generalised Estimating Equations was used to estimate the adjusted prevalence ratios (aPR) for the sex-specific factors associated with MetS. Overall, the prevalence of MetS was 34.0% (95% CI 32.4–35.6). MetS prevalence in women and men was 39.1% (95% CI 37.0–41.3) and 29.1% (95% CI 27.2–31.2), respectively. The most frequent triad was the clustering of abdominal obesity with low HDL-cholesterol and high blood pressure among women (8.9%; 95% CI [8.0–10.0]) and low HDL-cholesterol with high blood pressure and hyperglycaemia among men (5.2%; 95% CI [4.3–6.3]). Increasing age (aPR 3.21 [2.35–4.39] in men and aPR 3.47 [2.86–4.22] in women), cohabitation (aPR 1.14 [1.05–1.24]), women residing in urban areas (aPR 1.13 [1.01–1.26]), men with higher educational levels (aPR 1.39 [1.14–1.70]), and men with insufficient physical activity (aPR 1.16 [1.05–1.30]) were associated with higher risk of MetS. In this population-based study, one in three Algerian adults had MetS, and key components including abdominal obesity, low HDL-cholesterol, and high blood pressure, are very common, especially in women. Reinforcing interventions for weight management targeting married women living in urban areas and improving sufficient physical activity in men with higher socioeconomic status could provide maximal health gains and stem the CVD epidemic in Algeria.
High prevalence group testing in epidemiology with geometrically inspired algorithms
Authors: Schenk Hannes , Caf Yasemin , Knabl Ludwig , Mayerhofer Christoph , Rauch Wolfgang
Published: November 2023

Demand for mass surveillance during peak times of the SARS-CoV-2 pandemic caused high workload for clinical laboratories. Efficient and cost conserving testing designs by means of group testing can substantially reduce resources during possible future emergency situations. The novel hypercube algorithm proposed by Mutesa et al. 2021 published in Nature provides methodological proof of concept and points out the applicability to epidemiological testing. In this work, the algorithm is explored and expanded for settings with high group prevalence. Numerical studies investigate the limits of the adapted hypercube methodology, allowing to optimize pooling designs for specific requirements (i.e. number of samples and group prevalence). Hyperparameter optimization is performed to maximize test-reduction. Standard deviation is examined to investigate resilience and precision. Moreover, empirical validation was performed by elaborately pooling SARS-CoV-2 virus samples according to numerically optimized pooling designs. Laboratory experiments with SARS-CoV-2 sample groups, ranging from 50 to 200 items, characterized by group prevalence up to 10%, are successfully processed and analysed. Test-reductions from 50 to 72.5% were achieved in the experimental setups when compared to individual testing. Higher theoretical test-reduction is possible, depending on the number of samples and the group prevalence, indicated by simulation results.
Crossing fibers may underlie the dynamic pulling forces of muscles that attach to cartilage at the tip of the nose
Authors: Hur Mi-Sun , Lee Seunggyu , Jung Han-Sung , Schneider Richard A.
Published: November 2023

The present study used microdissection, histology, and microcomputed tomography (micro-CT) with the aims of determining the prevalence and patterns of the depressor septi nasi (DSN) and orbicularis oris (OOr) muscles attached to the footplate of the medial crus (fMC) of the major alar cartilage, focusing on their crossing fibers. The DSN and OOr attached to the fMC of the major alar cartilage were investigated in 76 samples from 38 embalmed Korean adult cadavers (20 males, 18 females; mean age 70 years). The DSN, OOr, or both were attached to the fMC. When the DSN ran unilaterally or was absent, some OOr fibers ascended to attach to the fMC instead of the DSN in 20.6% of the samples. Crossing fibers of the DSN or OOr attached to the fMC were found in 82.4% of the samples. Bilateral and unilateral crossing fibers were found in 32.4% and 50.0%, respectively, and no crossing fibers were found in 17.6%. The DSN and OOr that attached to the fMC could be categorized into six types according to presence of the DSN and the crossing patterns of the DSN and OOr. Anatomical findings of the DSN and OOr that attached to the fMC were confirmed in histology and micro-CT images. These findings offer insights on anatomical mechanisms that may underlie the dynamic pulling forces generated by muscles that attach to the fMCs and on evolutionary variation observed in human facial expressions. They can also provide useful information for guiding rhinoplasty of the nasal tip.
The relationship between myodural bridge, atrophy and hyperplasia of the suboccipital musculature, and cerebrospinal fluid dynamics
Authors: Yang Heng , Wei Xiao-Song , Gong Jin , Du Xue-Mei , Feng Hong-Bo , Su Chang , Gilmore Campbell , Yue Chen , Yu Sheng-Bo , Li Chan , Sui Hong-Jin
Published: November 2023

The Myodural Bridge (MDB) is a physiological structure that is highly conserved in mammals and many of other tetrapods. It connects the suboccipital muscles to the cervical spinal dura mater (SDM) and transmits the tensile forces generated by the suboccipital muscles to the SDM. Consequently, the MDB has broader physiological potentials than just fixing the SDM. It has been proposed that MDB significantly contributes to the dynamics of cerebrospinal fluid (CSF) movements. Animal models of suboccipital muscle atrophy and hyperplasia were established utilizing local injection of BTX-A and ACE-031. In contrast, animal models with surgical severance of suboccipital muscles, and without any surgical operation were set as two types of negative control groups. CSF secretion and reabsorption rates were then measured for subsequent analysis. Our findings demonstrated a significant increase in CSF secretion rate in rats with the hyperplasia model, while there was a significant decrease in rats with the atrophy and severance groups. We observed an increase in CSF reabsorption rate in both the atrophy and hyperplasia groups, but no significant change was observed in the severance group. Additionally, our immunohistochemistry results revealed no significant change in the protein level of six selected choroid plexus-CSF-related proteins among all these groups. Therefore, it was indicated that alteration of MDB-transmitted tensile force resulted in changes of CSF secretion and reabsorption rates, suggesting the potential role that MDB may play during CSF circulation. This provides a unique research insight into CSF dynamics.
A novel simulador for agile and graphical modeling of surface plasmon resonance based sensors
Authors: Gomes Julio C. M. , Oliveira Leiva C.
Published: November 2023

Surface plasmon resonance (SPR) sensor is a consolidated technology for analysis of biomolecular interaction, largely applied in biology and pharmaceutical research. The simulation of the surface plasmon optical excitation response is an important step in the development process of SPR based sensors. The structure, design and configuration of the desired sensor benefits from a previous simulated analyses of the generated responses, defining operational conditions and feasibility of the selected materials to composed the optical coupling layers. Here an online web-based SPR sensor’s simulator is presented. With a visual-oriented interface, enable drag & drop actions to easily and quickly model a variety of sensor arrangements. Presenting an embedded materials database for metals, glasses, 2D materials, nanoparticles, polymers, and custom substances, the simulator enables flexible configuration for sensors operating in angular and spectral modes, as well as localized SPR. The light propagation through the multilayer of materials is presented in terms of Fresnel coefficients, which are graphically displayed. The so-called SPR morphology parameters can be visualized. Moreover, sensor dynamic behavior could be knowledge by a Sensorgram simulation. Localized surface plasmon resonance (LSPR) in homogeneous and spherical nanoparticles is also present in the simulator. Simulated scenario’s in various configurations, designs and excitation were performed and compare with other simulator. The proposed simulator guarantees comparable results with low-code, agile, and intuitive flow of execution.
Desmoplasia in cervical cancer is associated with a more aggressive tumor phenotype
Authors: Wolf Benjamin , Weydandt Laura , Dornhöfer Nadja , Hiller Grit Gesine Ruth , Höhn Anne Kathrin , Nel Ivonne , Jain Rakesh K. , Horn Lars-Christian , Aktas Bahriye
Published: November 2023

In cancer of the uterine cervix, the role of desmoplasia, i.e., peritumoral stromal remodeling characterized by fibroblast activation and increased extracellular matrix deposition, is not established. We conducted a retrospective cohort study based on data from 438 patients who had undergone surgical treatment for cervical cancer as part of the prospective Leipzig Mesometrial Resection study between 1999 and 2021. Using non-parametric tests, Kaplan–Meier plotting, and Cox regression modeling, we calculated the prognostic impact of desmoplasia and its association with other risk factors. Desmoplasia was present in 80.6% of cases and was associated with a higher frequency of lymphovascular space involvement (76.5 vs. 56.5%, p < 0.001) and venous infiltration (14.4 vs. 2.4%, p < 0.001). Lymph node metastasis (23.0 vs. 11.8%, p < 0.05) and parametrial involvement (47.3 vs. 17.6%, p < 0.0001) were also more common in patients with desmoplasia. The presence of desmoplasia was associated with inferior overall (80.2% vs. 94.5% hazard ratio [HR] 3.8 [95% CI 1.4–10.4], p = 0.002) and recurrence-free survival (75.3% vs. 87.3%, HR 2.3 [95% CI 1.2–4.6], p = 0.008). In addition, desmoplasia was associated with significantly less peritumoral inflammation (rho − 0.43, p < 0.0001). In summary, we link desmoplasia to a more aggressive phenotype of cervical cancer, reduced peritumoral inflammation, and inferior survival.
Evaluating the prospective crown-root ratio after extrusion and crown lengthening procedures in vitro
Authors: Bruhnke Maria , Voß Isabelle , Sterzenbach Guido , Beuer Florian , Naumann Michael
Published: November 2023

For restoration of extensively damaged teeth preprosthetic treatment measures are necessary. Crown lengthening and extrusion affect the prospective crown-root ratio (CRR). The subject of this in vitro study was to compute CRRs for both treatment approaches. 120 human maxillary central extracted incisors were measured. Measurements were calculated for five treatment groups: C (control), E-2 mm (extrusion of 2 mm), E-4 mm (extrusion of 4 mm), CL-2 mm (crown lengthening of 2 mm), and CL-4 mm (crown lengthening of 4 mm). Tooth (TL), root (RL), and crown lengths (CL) were measured from mesial (m) and facial (f) cemento-enamel junction (CEJ), and respective anatomic (CRR) and effective crown-root ratios (eCRR) were calculated. Following CRR values were computed for C: CRR-m = 0.4 ± 0.1, CRR-f = 0.7 ± 0.1. All crown-root ratios were lower (more favourable) for extrusion compared to crown lengthening (p < 0.001). ECRRs were higher than anatomic CRRs. CRR at mesial CEJ was significantly lower than CRR with facial CEJ as reference (p < 0.001). Mesial measurement-based calculations of CRR typically based on radiographic images should be interpreted with caution as they underestimate the eCRR. CRR can be expected as lower, i.e. more favourable, when teeth are extruded than crown lengthened.
In vitro and in silico prediction of antibacterial interaction between essential oils via graph embedding approach
Authors: Yabuuchi Hiroaki , Hayashi Kazuhito , Shigemoto Akihiko , Fujiwara Makiko , Nomura Yuhei , Nakashima Mayumi , Ogusu Takeshi , Mori Megumi , Tokumoto Shin-ichi , Miyai Kazuyuki
Published: November 2023

Essential oils contain a variety of volatile metabolites, and are expected to be utilized in wide fields such as antimicrobials, insect repellents and herbicides. However, it is difficult to foresee the effect of oil combinations because hundreds of compounds can be involved in synergistic and antagonistic interactions. In this research, it was developed and evaluated a machine learning method to classify types of (synergistic/antagonistic/no) antibacterial interaction between essential oils. Graph embedding was employed to capture structural features of the interaction network from literature data, and was found to improve in silico predicting performances to classify synergistic interactions. Furthermore, in vitro antibacterial assay against a standard strain of Staphylococcus aureus revealed that four essential oil pairs (Origanum compactum—Trachyspermum ammi, Cymbopogon citratus—Thujopsis dolabrata, Cinnamomum verum—Cymbopogon citratus and Trachyspermum ammi—Zingiber officinale) exhibited synergistic interaction as predicted. These results indicate that graph embedding approach can efficiently find synergistic interactions between antibacterial essential oils.