bluuebunny/crossref_metadata_2025 huggingface dataset:
Synchronization and Bursting Activity in the Model for Two Predator-Prey Systems Coupled By Predator Migration
Authors: Kulakov M.P. , Kurilova E.V. , Frisman E.Ya.
Published: December 2019

The papers is devoted to a model for two non-identical predator-prey communities coupled by migration and characterized by logistic growth of prey and Holling type II functional response. The coupling is a predator migration at constant weak rate. The non-identity is the difference in the prey growth rates or predator mortalities in each patch. We studied the equilibrium states of model and scenarios of loss of their stability and emerge of complex periodic solutions. It was shown that in some domains of the parameter space there is a bursting activity which are that the dynamics of two communities contain segments of slowly resting dynamic (as part of a fast-slow cycle or canard) and regular bursts of spikes. In the resting part, the dynamics of the second community, as a rule, follow the slow changes in the first community, i.e. the dynamics in different patches are synchronous. But in the fast part there is only phase synchronization between the fast-slow cycle in first patch and bursts in second. We classified the scenarios of transition between different types of bursting activity by location spiking manifold and canard. These types differ not so much in size, shape or numbers of spikes as in the order of bursts emerge relative a slow-fast cycle. In a typical case the start of burst (divergent fast oscillations) coincides with the minimum numbers or quasi-extinction of prey in the first patch. After a rapid increase in the prey number in the first patch, diverging fluctuations give way to damped in the second patch. Such dynamics correspond to the rhombus-wave shape of spikes cluster. Another case is interesting, when the burst of spikes is formed after the full recovery of prey and with a certain predator number in the first patch. In this case, the spikes cluster takes the shape of a triangle-wave or a truncated rhombus-wave. It was shown that transitions between these types of bursts are accompanied by a change in the oscillation period and the degree of synchronization. Triangular-wave bursters correspond to complete synchronization of the predator dynamics in the resting part and rhomboid-wave correspond to antiphase synchronization. In the fast part with many spikes, communities are completely asynchronous to each other.
SATIRE OF THE UKRAINIAN DIASPORA OF THE 30’S AND 40’S OF 20TH CENTURY: STEFAN FODCHUK AND YAKIV MAYDANYK
Authors: Osoba Natalia
Published: December 2019

In the article the features of the Ukrainian diaspora satirical and humorous literature of the 20’s and 40’s of the XX century are investigated, its thematic filling and specific features on the example of the works of satirists Stefan Fodchuk and Yakiv Maydanyk are indicated. For the first time, an attempt to analyze the works of humorists, who by virtue of certain circumstances were in emigration, was made; the specifics of the development of humorous and satirical genres, in particular, mini-feuilleton, mini-humoresque, fable, peculiarities of social and moral conflicts in their creativity are determined. It is emphasized that their works have expressed aesthetic and educational value. Responding to the evil of the day, humorists tried to touch on the most important problems of life, penetrated into the depths of phenomena, into their innermost essence, cares and pity of the emigrants. In their creative heritage mini-humoresques, mini-feuilletons, anecdotes, witty poetic chetverics, epigrams and epitaphs, parodies, fables, puns and aphorisms are singled out. The satirists were able to compress the phrase to an incredible, often explosive density.

It is noted that the research of the Ukrainian satire of the interwar period, which by the number of raised themes and problems is exceptionally diverse and rich, reveals to the researcher the great opportunities and prospects for reconstruction of the return of the so-called lost reality, which presents a large array of satirical literature of the Ukrainian diaspora, which has not yet been restored in a holistic and undistorted accents of colonial discourse form in our cultural and historical space as one of the important steps in search of a national identity.
THE CAMP CHRONOTOPE IN THE ARTISTIC COMPREHENSION OF THE PRAGUE LITERARY SCHOOL REPRESENTATIVE
Authors: Prosalova Vira
Published: December 2019

The article highlights the peculiarities of artistic comprehension of the camp chronotope by representatives of the Prague Literary School: Yevgen Malaniuk, Yuri Lypa, Galia Mazurenko, Yuri Daragan. The background for conducting the current study includes the lyric poem ‘U shpitali’ by Y. Daragan (Eng. In the Hospital), epic works, namely the essay ‘Tabir’ by Y. Lypa (Eng. The Camp) and the story of G. Mazurenko ‘Ne tot kozak, khto poborov, a tot kozak, khto ‘vyvernet’sya’...’ (Eng. Not that Cossack, who overpowered, but that Cossack, who ‘will dodge’) and the diary entries. The camp in the works of these authors is regarded as someone else’s space, closed, restricted, isolated and hostile. This space, fenced by the wires, is opposed to the home land as desired, open and inaccessible.

The differences in the representation of the camp chronotope by different authors were revealed; it is found that the per- ception of camps for interned soldiers in Poland was influenced primarily by the age of the writer, the duration and place of his detention. The camp in Kalisha favorably differed from other detention places of interned by more well-adjusted life, numerous events in the cultural life, lively activity of the literary and artistic societies.

Yevgeny Malaniuk and Yuri Lypa’s impressions about staying in a camp for interned Ukrainian soldiers in Kalisha are compared. The paper reveals that the writers have presented the reflection of the collective and personal experience of staying in the places of reservation, created a series of narratives that confirm the real threat of loss (according to Merab Mamardashvili) of the “ontological rooting in the world”.

The difference in the perception of the camps’ conditions by Yuri Lypa and Yevgen Malaniuk is mainly due to the long detention of the latter in Kalisha, as well as the careful search for answers to the question about causes of the tragic defeat in the national liberation struggle, the critical attitude towards their compatriots.
Design of physical access control system with integrated wireless authentication
Authors: Godovnikov Evgeny A. , Shicelov Anatoliy V. , Usmanov Ruslan T.
Published: December 2019

This article discusses the design of a physical access control system for an enterprise with various methods of authentication and user identification. A review of existing solutions in the design of physical access control systems was conducted. In the course of the work, a system design was proposed, and its components were described in detail.
Long-term results of surgical treatment of thyroid adenomas
Authors: Artyukhin Stanislav Olegovich , Aristarhov Vladimir Georgievich , Puzin Denis Anatolyevich
Published: December 2019

Aim. To study the long-term results of surgical treatment of patients with thyroid adenomas.
Methods. From 2004 to 2006, 667 patients with follicular adenomas of the thyroid gland, including 134 (20.1%) men and 533 (79.9%) women, were operated on in the surgical department №2 of city clinical hospital №11 of Ryazan. 3 groups of patients were defined: group 1 operated on for solitary adenomas of the thyroid gland, 103 patients, hemithyreoidectomy performed; group 2 operated on for multiple adenomas of the thyroid in both lobes, 101 patients, thyroidectomy performed; group 3 32 patients operated on for multiple adenomas of the thyroid in the same lobe, hemithyreoidectomy performed. Patients were examined 14 years after the surgery.
Results. Hypothyroidism was diagnosed in: group 1 18 (17.5%) patients, group 2 101 (100%), group 3 0. All patients with hypothyroidism were compensated with hormone replacement therapy. Permanent hypoparathyroidism was diagnosed only in group 2 8 (4.8%) patients. Unilateral laryngeal paralysis was found in group 1 in 1 (0.9%) patient, group 2 in 2 (1.9%) patients, group 3 contained no such patients. Recurrence (node more than 1 cm according to ultrasound) was observed in group 1 in 12 (11.7%) patients; fine-needle aspiration found colloidal nodes in 8 (7.8%) patients (Bethesda II), in 4 (3.9%) follicular tumor (IV). Group 2 had no recurrences. In group 3 11 (34.3%) patients had recurrence; fine-needle aspiration found colloidal nodes in 1 (3.1%) patient (Bethesda II), in 10 (31.3%) follicular tumor (Bethesda IV).
Conclusion. In solitary adenomas it is possible to perform organ-saving operations, which reduces the percentage of postoperative complications; in multiple adenomas, the optimal volume of surgery is thyroidectomy; when multiple adenomas are localized in one lobe, the chance of recurrence of the disease (31.3%) is high leading to re-surgery.
A novel extraction method for weld defect segmentation seeds using ANDM and clustering
Authors: Dang Changying , Li Jiansu , Du Wenhua , Zeng Zhiqiang , Wang Rijun
Published: December 2019

To improve the accuracy and reliability in extracting defect segmentation seeds from a weld radiographic testing (RT) image, a novel extraction method (NESS) using clustering and a novel defect detection method (ANDM) that was presented in a previous paper by one of the authors is proposed
in this paper. In the proposed NESS, firstly each column of the weld RT image is accurately analysed by ANDM to judge whether or not it really passes through weld defect regions. Most importantly, one or more defect seeds can be acquired if it passes through a defect region. Secondly, all
the defect seeds (a defect seed group) of the RT image are extracted by analysing the entire image. Finally, a sorting-based clustering method is proposed to quickly and accurately search for defect segmentation seeds among all the defect seeds, which can solve the problems concerning the
difficulty in determining defect segmentation seeds and the heavy calculational burden of defect segmentation. In order to evaluate the performance of the proposed NESS, some clustering and segmentation experiments have been performed. The experimental results reveal that the proposed NESS
achieves high accuracy and reliability in extracting defect segmentation seeds from RT images and is helpful in defect segmentation.
CONSTRUCTION OF INFORMATION SYSTEMS OF KNOWLEDGE FORMATION ON THE BASIS OF SERVICE-ORIENTED ARCHITECTURE USING NEURAL NETWORKS
Authors: OMAROV Murad , МУРАДОВА вюсаля
Published: December 2019

The subject of research is the design and development of information systems and management processes of knowledge formation. Objective is to analyze the modern information technologies and service-oriented systems used to organize and control the processes of formation of knowledge as well as practical and methodological approaches used in these technologies. The following tasks are solved in the article: development of information technology, organization and management of the processes of knowledge formation on the basis of methods and models of service-oriented systems. This technology is based on the functional requirements of the processes of knowledge formation. The following results were obtained: The relevance of the scientific objectives was analyzed. The results of the analysis of the modern models, methods and information technologies in processes of knowledge formation confirmed the need for the development of information technology processes control the formation of knowledge, which will improve the effectiveness of decisions. It was identified that this technology needs to be developed based on the service oriented architecture.
Conclusions: The information technology management processes of knowledge formation based on service-oriented architecture with the use of new methods and models of processes of formation of knowledge was developed.
Riesgo de crecida por rompimiento de diques de hielo en la cuenca del plomo, Andes Centrales (33º S): perspectivas a partir de eventos históricos
Authors: Correas-Gonzalez M. , Moreiras S.M. , Jomelli V. , Arnaud-Fassetta G.
Published: December 2019

Las regiones montañosas son altamente sensibles a los cambios climáticos actuales, afectando los procesos naturales y los peligros asociados (i.e. retroceso glaciar, desestabilización de laderas, formación y/o agrandamiento de lagos proglaciares). Destacan entre estos eventos las crecidas abruptas producto del colapso de diques de hielo glaciar, conocidas por su acrónimo en inglés como IDLOF (Ice-Dammed Lake Outburst Flood), que implican un gran impacto socioeconómico debido a su alto poder destructivo. Frente a los pronósticos de aumento en frecuencia e intensidad de eventos potencialmente peligrosos, se hace necesario el análisis del riesgo al cual están expuestas las comunidades locales. El objetivo de esta investigación fue evaluar la peligrosidad, vulnerabilidad y el riesgo de IDLOF en los valles de los ríos Plomo y Mendoza en escenarios actuales. Para ello analizamos diferentes eventos IDLOF ocurridos en el río Plomo que fue represado en al menos tres ocasiones por el glaciar en surge Grande del Nevado del Plomo (GNP) produciendo seis crecidas en el período 1788-2018. Casi un siglo después del evento más catastrófico ocurrido en 1934, han tenido lugar grandes cambios en la ocupación y usos del suelo del valle del río Mendoza, exponiendo nuevos elementos vulnerables a los peligros naturales de la región. Nuestros resultados evidencian un mayor grado de riesgo debido al aumento de la vulnerabilidad como consecuencia de una mayor exposición del hábitat asociado principalmente a la creciente actividad turística y nuevos usos del suelo. Esto pone de manifiesto la necesidad urgente de implementar medidas de ordenación territorial en las regiones andinas.
A comparative study on the use of holmium laser and pneumatic lithotripser for intracorporeal lithotripsy of upper ureteric stones
Authors: Sharma Elias , Langer Raja , Langer Bhavna
Published: December 2019

Background: In the last decade different minimally invasive modalities for management of urolithiasis have been available requiring an intelligent decision by the urologist to choose one as per patients need and preferences.Methods: The present cross sectional study was conducted among patients who underwent ureterorenoscopic lithotripsy for proximal ureteric stones in the Department of Urology. Using consecutive sampling technique, patients were randomly allocated to two groups for treatment with either pneumatic lithotripser (group A) or Holmium laser lithotripser for fragmentation of calculi (group B) and the comparison of both these techniques was done.Results: 117 patients were included (57 and 60 in group A and group B respectively) in the study. The difference in age in the two groups was found to be statistically significant (p=0.03). The total operative time, length of stay in hospital and complications were more in group A patients. The difference in two groups for total operative time and length of stay in hospital was statistically significant (p=0.00 and 0.00 respectively).Conclusions: Holmium laser lithotripser has better safety profile when compared to pneumatic lithotripser especially in stone clearance rate and retropulsion rates and could be used as modality of choice in proximal ureteric stones.
A new design micropatch antenna for GPS applications on vehicle navigation systems
Authors: İMAMOĞLU Fatih , KARPAT Esin
Published: December 2019

A
new design of the micropatch antenna for GPS applications is studied. 70
antenna iterations working around the L1 band (1575 MHz) with different sized
quarter-circular slots on the patch are designed and simulated. In parallel,
“design dimensional parameters (“r” slot radius and “h” substrate thickness)
and antenna resonant frequencies according to parameters” are applied to curve
fitting algorithm and an equation for resonance frequency is obtained based on
r and h. The resonance frequency values obtained by proposed equation are compared
with simulations.
Structural determinants of cooperativity in acto-myosin interactions.
Authors: Moraczewska Joanna
Published: December 2019

Regulation of muscle contraction is a very cooperative process. The presence of tropomyosin on the thin filament is both necessary and sufficient for cooperativity to occur. Data recently obtained with various tropomyosin isoforms and mutants help us to understand better the structural requirements in the thin filament for cooperative protein interactions. Forming an end-to-end overlap between neighboring tropomyosin molecules is not necessary for the cooperativity of the thin filament activation. When direct contacts between tropomyosin molecules are disrupted, the conformational changes in the filament are most probably transmitted cooperatively through actin subunits, although the exact nature of these changes is not known. The function of tropomyosin ends, alternatively expressed in various isoforms, is to confer specific actin affinity. Tropomyosin's affinity or actin is directly related to the size of the apparent cooperative unit defined as the number of actin subunits turned into the active state by binding of one myosin head. Inner sequences of tropomyosin, particularly actin-binding periods 3 to 5, play crucial role in myosin-induced activation of the thin filament. A plausible mechanism of tropomyosin function in this process is that inner tropomyosin regions are either specifically recognized by myosin or they define the right actin conformation required for tropomyosin movement from its blocking position.
Actin in human colon adenocarcinoma cells with different metastatic potential.
Authors: Nowak Dorota , Krawczenko Agnieszka , Duś Danuta , Malicka-Błaszkiewicz Maria
Published: December 2019

Four human colon adenocarcinoma cell line variants with different metastatic potential were used to examine whether a correlation exists between actin level, state of actin polymerization and invasiveness of tumour cells. Monomeric (G), total (T) and filamentous (F) actin were determined in the cytosolic fraction of these cells. A statistically significant decrease in G actin level and increase in the state of actin polymerization (measured by F:G actin ratio) were found in the cytosol of three cell variants with higher metastatic potential and invasiveness (EB3, 3LNLN, 5W) compared with the parental cell line (LS180). Our experimental data lead to the conclusion that there is a correlation between the metastatic capacity of human colon adenocarcinoma cells and the state of actin polymerization.
Myosin molecule packing within the vertebrate skeletal muscle thick filaments. A complete bipolar model.
Authors: Skubiszak Ludmila , Kowalczyk Leszek
Published: December 2019

Computer modelling related to the real dimensions of both the whole filament and the myosin molecule subfragments has revealed two alternative modes for myosin molecule packing which lead to the head disposition similar to that observed by EM on the surface of the cross-bridge zone of the relaxed vertebrate skeletal muscle thick filaments. One of the modes has been known for three decades and is usually incorporated into the so-called three-stranded model. The new mode differs from the former one in two aspects: (1) myosin heads are grouped into asymmetrical cross-bridge crowns instead of symmetrical ones; (2) not the whole myosin tail, but only a 43-nm C-terminus of each of them is straightened and near-parallel to the filament axis, the rest of the tail is twisted. Concurrent exploration of these alternative modes has revealed their influence on the filament features. The parameter values for the filament models as well as for the building units depicting the myosin molecule subfragments are verified by experimental data found in the literature. On the basis of the new mode for myosin molecule packing a complete bipolar structure of the thick filament is created.
The vertebrate skeletal muscle thick filaments are not three-stranded. Reinterpretation of some experimental data.
Authors: Skubiszak Ludmila , Kowalczyk Leszek
Published: December 2019

Computer simulation of mass distribution within the model and Fourier transforms of images depicting mass distribution are explored for verification of two alternative modes of the myosin molecule arrangement within the vertebrate skeletal muscle thick filaments. The model well depicting the complete bipolar structure of the thick filament and revealing a true threefold-rotational symmetry is a tube covered by two helices with a pitch of 2 x 43 nm due to arrangement of the myosin tails along a helical path and grouping of all myosin heads in the crowns rotated by 240 degrees and each containing three cross-bridges separated by 0 degrees, 120 degrees, and 180 degrees. The cross-bridge crown parameters are verified by EM images as well as by optical and low-angle X-ray diffraction patterns found in the literature. The myosin tail arrangement, at which the C-terminus of about 43-nm length is near-parallel to the filament axis and the rest of the tail is quite strongly twisted around, is verified by the high-angle X-ray diffraction patterns. A consequence of the new packing is a new way of movement of the myosin cross-bridges, namely, not by bending in the hinge domains, but by unwrapping from the thick filament surface towards the thin filaments along a helical path.
p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein (GCAP).
Authors: Dejda Agnieszka , Matczak Izabela , Gorczyca Wojciech A
Published: December 2019

The Ca(2+)-dependent activation of retina-specific guanylyl cyclase (retGC) is mediated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 microM) concentrations of Ca(2+). At low Ca(2+), immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat retinal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca(2+)-dependent electrophoretic mobility shift.
The effect of vanadate on Pichia pastoris growth, protein kinase A activity and ribosomal protein phosphorylation.
Authors: Frajnt Magdalena , Cytryńska Małgorzata , Jakubowicz Teresa
Published: December 2019

It was found that wild type yeast Pichia pastoris can tolerate vanadate concentration as high as 25 mM in the growth medium. Moreover, four vanadate-resistant P. pastoris strains designated JC100/1, JC100/3, JC100/9 and JC100/15 exhibiting tolerance up to 150 mM vanadate were selected. Growth of P. pastoris was correlated with vanadate to vanadyl reduction and its accumulation in the growth medium. In two selected strains, JC100/9 and JC100/15, protein kinase A activity was much higher in comparison to the wild type strain even without vanadate addition to the growth medium. Moreover, in the presence of vanadate, protein kinase A activity was significantly increased in the wild type and the vanadate-resistant JC100/1 and JC100/3 strains. It was also found that phosphorylation of a 40 kDa protein associated with ribosomes occured in all vanadate-resistant strains from the logarithmic, while in the wild type strain from the stationary growth phase. From the presented results it can be concluded that a protein kinase A signalling pathway(s) might be involved in the mechanism of P. pastoris vanadate resistance. The results also indicate a possible role of the 40 kDa protein in protection of P. pastoris against vanadate toxicity.
Up-regulation of spermidine/spermine N1-acetyltransferase (SSAT) expression is a part of proliferative but not anabolic response of mouse kidney.
Authors: Dudkowska Magdalena , Stachurska Agnieszka , Grzelakowska-Sztabert Barbara , Manteuffel-Cymborowska Małgorzata
Published: December 2019

A differential expression pattern of spermidine/spermine N(1)-acetyltransferase (SSAT), the enzyme critical to proper homeostasis of cellular polyamines, is reported in mouse kidney undergoing hyperplasia and hypertrophy. We have shown that SSAT activity and SSAT mRNA are significantly induced by antifolate CB 3717 and folate that evoke a drug-injury-dependent hyperplasia. In contrast, SSAT activity is down-regulated in the testosterone-induced hypertrophic kidney, while SSAT mRNA is positively controlled by this androgen. Catecholamine depletion evoked by reserpine drastically decreases the folate-induced activity of S-adenosylmethionine decarboxylase (AdoMetDC), which limits polyamine biosynthesis, but has no effect on SSAT activity augmented by CB 3717. Our results document that the increased SSAT expression solely accompanies the proliferative response of mouse kidney, and suggest the importance of post-transcriptional regulation to the control of SSAT activity in both hyperplastic and hypertrophic experimental models.
IGF-I: from diagnostic to triple-helix gene therapy of solid tumors.
Authors: Trojan Ladislas A , Kopinski Piotr , Wei Ming X , Ly Adama , Glogowska Aleksandra , Czarny Jolanta , Shevelev Alexander , Przewlocki Ryszard , Henin Dominique , Trojan Jerzy
Published: December 2019

Alterations in the expression of growth factors and their receptors are associated with the growth and development of human tumors. One such growth factor is IGF-I (insulin-like growth factor I ), a 70-amino-acid polypeptide expressed in many tissues, including brain. IGF-I is also expressed at high levels in some nervous system-derived tumors, especially in glioblastoma. When using IGF-I as a diagnostic marker, 17 different tumors are considered as expressing the IGF-I gene. Malignant glioma, the most common human brain cancer, is usually fatal. Average survival is less than one year. Our strategy of gene therapy for the treatment of gliomas and other solid tumors is based on: 1) diagnostic using IGF-I gene expression as a differential marker, and 2) application of "triple-helix anti-IGF-I" therapy. In the latter approach, tumor cells are transfected with a vector, which encodes an oligoribonucleotide--an RNA strand containing oligopurine sequence which might be capable of forming a triple helix with an oligopurine and/or oligopyrimidine sequence of the promotor of IGF-I gene (RNA-IGF-I DNA triple helix). Human tumor cells transfected in vitro become down-regulated in the production of IGF-I and present immunogenic (MHC-I and B7 expression) and apoptotic characteristics. Similar results were obtained when IGF-I antisense strategy was applied. In both strategies the transfected cells reimplanted in vivo lose tumorigenicity and elicit tumor specific immunity which leads to elimination of established tumors.
Carbohydrate moieties of N-cadherin from human melanoma cell lines.
Authors: Ciołczyk-Wierzbicka Dorota , Gil Dorota , Hoja-Łukowicz Dorota , Lityńska Anna , Laidler Piotr
Published: December 2019

Expression of N-cadherin an adhesion molecule of the cadherin family, in tumor cells is associated with their increased invasive potential. Many studies suggested the role of N-linked oligosaccharides as important factors that contribute to metastasis by influencing tumor cell invasion and adhesion. N-cadherin is a heavily glycosylated protein. We have analysed the carbohydrate profile of this protein synthesized in human melanoma cell lines: WM35 from the primary tumor site and WM239, WM9, and A375 from different metastatic sites. N-cadherin was immunoprecipitated with anti-human N-cadherin polyclonal antibodies. Characterisation of its carbohydrate moieties was carried out by SDS/PAGE electrophoresis and blotting, followed by immunochemical identification of the N-cadherin polypeptides and analysis of their glycans using highly specific digoxigenin or biotin labelled lectins. The positive reaction of N-cadherin from the WM35 cell line with Galanthus nivalis agglutinin (GNA), Datura stramonium agglutinin (DSA) and Sambucus nigra agglutinin (SNA) indicated the presence of high-mannose type glycans and biantennary complex type oligosaccharides with alpha2-6 linked sialic acid. N-cadherin from WM239, WM9, and A375 cell lines gave a positive reaction with Phaseolus vulgaris leukoagglutinin (L-PHA) and lotus Tetragonolobus purpureas agglutinin (LTA). This indicated the presence of tri- or tetra-antennary complex type glycans with alpha-fucose. In addition, N-cadherin from WM9 (lymphomodus metastatic site) and A375 (solid tumor metastatic site) contained complex type chains with alpha2-3 sialic acid (positive reaction with Maackia amurensis agglutinin--MAA). The results demonstrated that N-glycans of N-cadherin are altered in metastatic melanomas in a way characteristic for invasive tumor cells.
The mechanism of azide activation of polyphenol oxidase II from tobacco.
Authors: Shi Chunhua , Liu Qingliang , Dai Ya , Xie Yongshu , Xu Xiaolong
Published: December 2019

So far, azide has been consistently reported to act as an inhibitor of metal enzymes, especially copper proteins. The present work shows that azide can also act as an activator of polyphenol oxidase II (PPO II) from tobacco leaves. From the square-wave voltammetry of native PPO II, peroxide-PPO II complex and azide-PPO II complex, the reduction of nitro blue tetrazolium by the enzymes and activation of PPO II by peroxide it follows that the binding of azide to PPO II induces the formation of CuO(2)(2-)Cu in the active site of PPO II from CuO(2)(-)Cu in native PPO II. The reason for azide acting as an activator can be attributed to azide complexing with PPO II, thus inducing the formation of CuO(2)(2-)Cu, which is the active site of the peroxide-PPO II complex in which peroxide plays the role of activator.
RFLP analysis of 1-aminocyclopropane-1-carboxylate synthase ACC2 and ACC4 genes from Polish cultivars of tomato.
Authors: Jakubowicz Małgorzata , Jółkowska Justyna , Kwapiszewska Grazyna , Krecisz Anna , Czerski Piotr
Published: December 2019

An important trait of tomato is the rate of fruit ripening, strongly dependent on ethylene production. The ripening-related ethylene synthesis in tomato is controlled mainly by 1-aminocyclopropane-1-carboxylate synthase LE-ACS2 and LE-ACS4 isoenzymes (Rottmann et al., 1991, J. Mol. Biol. 222: 937; Lincoln et al., 1993, J. Biol. Chem. 268: 19422; Barry et al., 2000, Plant Physiol. 123: 979). In spite of numerous reports on the LE-ACS2 and LE-ACS4 gene expression, only ones considered the genomic organisation each of these genes (Rottmann et al., 1991; Lincoln et al., 1993) reported one copy of each of these genes in tomato cv VF36. In this article we suggest that the genomic organisation of LE-ACS2 and LE-ACSS4 genes may depend on tomato cultivars and may differ from that described by the above authors. The results of Southern analyses of genomic DNAs from 17-day old seedlings (cultivars Jaga, Halicz, Betalux, New Yorker) imply that the genomic organisation of LE-ACS2 and LE-ACS4 genes in Polish cultivars differs from that reported for cv VF36.
Editorial
Authors: Casadei Eliza Bachega , Nunes Mônica Rebecca Ferrari
Published: December 2019

Editorial
Peran Pola Asuh Orangtua di Era Digital
Authors: Aslan Aslan
Published: December 2019

Technological developments are increasingly rapid in the digital age today, causing the values that are born, both positive and negative also experience a tremendous surprise for humans. From these surprises, so the role of parents in educating their children also changes by the development of their times. In the 80, parents in educating their children must have experienced a difference in the digital age today. The parenting style that initially experienced differences from other parents, which only applied authoritarian, permissive, democratic types of parenting, had experienced success in educating children, but in the age of the digital era, the three parenting styles would not succeed, if they did not synchronize according to the time of the situation and conditions in terms of parenting.
El bioarte a la luz de la propiedad intelectual
Authors: Rengifo Martínez José Daniel , Mendieta Chaparro Luisa María
Published: December 2019

Este artículo de revisión tiene como objetivo plantear los retos del bioarte frente a la propiedad intelectual, y, en consecuencia, las barreras que estos artistas pueden encontrar tanto en la protección de sus obras, como en el uso de tecnologías protegidas a la hora de desarrollar sus procesos creativos. Para esto, se analizan los conceptos y técnicas usadas en varias obras de bioarte creadas desde finales del siglo xx frente a los postulados tradicionales de la propiedad intelectual, de acuerdo con la normatividad de la CAN, así como jurisprudencia y doctrina nacional e internacional. En tanto el bioarte es producto de una relación compleja entre arte y ciencia, el artículo se divide en dos bloques: el primero estudia el bioarte desde el Derecho de autor, abarcando la no protección de las ideas, irrelevancia del mérito y criterios de originalidad, creación intelectual, y fijación, y, el segundo lo estudia desde el Derecho de patentes, abarcando elegibilidad, criterios de patentabilidad y libertad de operación. También se incluyen reflexiones en torno a la discusión bioética y su relación con el objeto de estudio.
The effect of parental education levels on children’s rights knowledge levels and attitude
Authors: Elci Ebru , Kuloglu Cigdem
Published: December 2019

The purpose of this study is to reveal the relationship between the attitudes of parents toward children’s rights and educational levels of their parents. The researcher’s universe consists of parents who live in Istanbul and has children between the ages of 6 and 14, and sampling consists of a total of 3100 parents (1550 mothers and 1550 fathers). The data of the study were collected with a Likert type Parent-Child Rights Attitude Scale consisting of 63 items. The scale evaluates the attitudes of the parents toward their children’s rights as two main attitudes, ‘Care and Protection’ and ‘Self-Determination’. In ‘Care and Protection’ attitude, there are two sub-dimensional structures as ‘Government Assurance and Support’ and ‘Care and Protection’. The ‘Self-Determination’ attitude has a single sub-dimensional structure. In the analysis of data, Statistical Package for the Social Sciences program was used beside necessary statistical techniques. The data obtained regarding the effect of parents' education levels on their attitudes toward children’s rights are presented in a tabular form with respective frequencies. Keywords: Children’s rights, parent’s attitudes toward child rights, parent’s attitudes
Kuyper and vaccinations a case study in Kuyper’s approach to an ethical issue
Authors: Bishop Steve
Published: December 2019

The 2019 translation of the second volume of Abraham Kuyper’s Pro Rege contains chapters on his approach to vaccinations. This article examines his arguments in favour of vaccinations as a case study on Kuyper’s approach to an ethical issue. Kuyper utilizes both sphere sovereignty and common grace as the key philosophical tools as well as using scriptural, rhetorical and pragmatic arguments to make his case.
TÜRKİYE’DE KAMU KURUMLARI İÇ DENETİM FAALİYETLERİ SÜRECİNİN AVRUPA KOMİSYONU İLERLEME RAPORLARI KAPSAMINDA DEĞERLENDİRİLMESİ
Authors: YILMAZ İlyas , GÜNEŞ Recep
Published: December 2019

Dünya’da kamu mali yönetim anlayışında meydana gelen önemli değişimler, Türkiye’de de kamu
sektörüne yansıyarak yeni bir kamu mali yönetim kanunun kabul edilmesini gerekli kılmıştır. Bu
nedenle 2003 yılında 5018 sayılı Kamu Mali Yönetimi ve Kontrol Kanunu kabul edilmiştir. Söz
konusu Kanunun getirdiği en önemli yeniliklerden biri, kamu kurumlarında iç denetim
faaliyetlerine başlanmasıdır. Avrupa Komisyonu, 1998 yılından itibaren aday ülkelerin
Kopenhag kriterlerine uyum konusunda kaydettiği gelişmeleri yıllık olarak değerlendiren
raporlar yayımlamaktadır. Söz konusu raporlar 2015 yılına kadar “İlerleme Raporu”, sonrasında
ise “Ülke Raporu” olarak adlandırılmıştır. Bu makalenin amacı, 5018 sayılı Kamu Mali Yönetim
ve Kontrol Kanunun, kamu kurumlarında iç denetim faaliyetlerinin gelişimini AB standartlarına
uyumu açısından değerlendirmektir. Bu amaçla, 518 sayılı kanunun uygulamaya başlamasından
günümüze kadar geçen süre içerisinde, konu ile ilgili Avrupa Birliği ilerleme raporları
incelenerek değerlendirmeler yapılmıştır..
Effectiveness of Leaves Extract Fractions of Archidendron jiringa (Jack) I.C Nielsen Against Microbes
Authors: Komala Oom , Wardatun Sri , Sari Lia Puspita
Published: December 2019

Archidendron jiringa(Jack) I.C Nielsen) contains a lot of essential oils, saponins, alkaloids, terpenoids, steroids, tannins, glycosides, and flavonoids. Flavonoid is a secondary metabolite compound in a plant as antimicrobial. This study aims to determine the antimicrobial activity of fractions of ethanol 96%, n-hexan, ethyl acetate, and ethanol-water leaves extractand determine levels of favonoids.  A. jiringaleaves extract were tested against Streptococus mutans, Pseudomonas aeruginosa, and Candida albicans.  The research using agar disc diffusion technique for antimicrobial  activity and colorimetric method to know the total of flavonoid. Chloramphenicol and ketoconazole were used as a reference standard. The result of this study shows fractions of ethanol 96%,  n-hexane,  ethyl acetate, and  ethanol-water A. jiringaleaves extract  can inhibit microbial growth of S. mutans, P. aeruginosa, and C. albicans.Total of flavonoids on the extract respectively are 1.13%, 0.494%, 2.337%, and 0.549%. determined with complementary colorimetry to each leaf extract fractionA. jiringawith aluminum chloride method. Absorption spectrum measurement using a spectrophotometer.  The higher  percent of flavonoids would cause greater    bacteriainhibitory  zone but  only  0.090 for  pearson correlation value. Conclusions ethyl acetate leaves extract most effective inhibit microbial growth from the other fractions.
Improving Irrigation Water Use Efficiency of Robusta Coffee (Coffea canephora) Production in Lam Dong Province, Vietnam
Authors: Tien Nguyen Dinh , Duyen Tran Nhat Lam , Thuy Pham Thu , Rañola Jr. Roberto F. , Thinh Nguyen An
Published: December 2019

Recent prolonged dry periods and lack of irrigation water have severely affected the productivity of coffee farms’ in the Central Highlands of Vietnam. This paper analyzes the efficiency of irrigation water use for Robusta coffee (Coffea canephora) in Lam Dong province, Highlands, Vietnam. A Cobb-Douglas production function was used to determine coffee productivity’s response to the application of irrigation water and other production factors using data collected from 194 farmers while the Technical Efficiency (TE) and Irrigation Water Use Efficiency (IWUE) were analyzed using a Data Envelopment Analysis (DEA) model. The correlation of different factors to IWUE was determined using the Tobit model. The production function analysis using Cobb-Douglas shows that the volume of irrigation water, amount of working capital, labor and farm size significantly influence coffee productivity. It also shows that indigenous farmers are more efficient in utilizing irrigation water than the (mostly Kinh) migrant farmers. The Tobit result, on the other hand. indicates that farmers’ experience, education level, distance of farm to water source, security of access to water source, extension contact and credit access significantly affect IWUE. The study findings further suggest that mitigating water shortages in coffee farms require sub-regional and national policy support such as better access to credit and extension services, training, land management and household-level effort to improve farming practices, through the application of appropriate technologies and traditional knowledge.
Dark Energy Stars with Quadratic Equation of State
Authors: Malaver Manuel , Kasmaei Hamed
Published: December 2019

Recent astronomical observations with respect to measurements in distant supernovas, cosmic microwave background and weak gravitational lensing confirm that the Universe is undergoing a phase of accelerated expansion and it has been proposed that this cosmological behavior is caused by a hypothetical dark energy which has a strong negative pressure that allows explain the expanding universe. Several theoretical ideas and models related dark the energy includes the cosmological constant, quintessence, Chaplygin gas, braneworld and tachyonic scalar fields. In this paper, we have obtained new relativistic stellar configurations considering an anisotropic fluid distribution with a charge distribution which could represents a potential model of a dark energy star. In order to investigate the effect of a quadratic equation of state in this anisotropic model we specify particular forms for the gravitational potential that allow solving the Einstein-Maxwell field equations. For these new solutions we checked that the radial pressure, metric coefficients, energy density, anisotropy factor, charge density , mass function are well defined and are regular in the interior of the star. The solutions found can be used in the development of dark energy stars models satisfying all physical acceptability conditions but the causality condition and strong energy condition are violated. We expect that these models have multiple applications in astrophysics and cosmology.
Comparative Analysis of Risk Management Techniques for Large-Scale Systems
Authors: Sirshar Mehreen , Shahid Aliya , Alam Zoya
Published: December 2019

Risk assessment management have been a hot topic for the researchers since a very long time. Software risk management is an important part of project management as it contains the identification, analysis, estimation and monitoring of different risks present in the system. This helps developers in decision making while assessing the problems that could arise in the software systems. Risk management is very complex in large scale system as these systems have very complex development. The paper describes risk management techniques for large scale system. Furthermore we have provided a detailed comparative analysis of these techniques with commonly identified risks in software systems and have provided a systematic order for risk management process to ensure risk mitigation.
ASPEK DIKSI SERTA NILAI-NILAI PENDIDIKAN KARAKTER DALAM NOVEL AYAH KARYA ANDREA HIRATA
Authors: Rizqi Annis Kurniyati , Suwandi Sarwiji , Suhita Raheni
Published: December 2019

This study aimed to describe and explain: (1) the aspects of diction used in the novel Ayah by Andrea Hirata; (2) the values of character education in the novel Ayah by Andrea Hirata; and (3) the relevance of Andrea Hirata’s novel Ayah as a teaching material of language and literature in Vocational High School. This study is a qualitative research that analyzes the data in the form of documents with the object of study of novel Ayah by Andrea Hirata. The results of this study are as follows. (1) The diction aspect contained in Andrea Hirata's father's novel includes five types of diction, (2) Novels contain sixteen points of character education values, (3) Novel Ayah is relevant to the learning of Indonesian language and literature in SMK because the aspect of diction and the value of character education in accordance with the learning objectives that have been designed using the Learning Implementation Plan based on the Core Competence and Basic Competence which includes the competencies that the learners must achieve in language and literature learning. Dad's novel can increase students' vocabulary mastery according to language ability, student psychology, and cultural background.
ANALISIS PSIKOLOGI SASTRA DAN NILAI PENDIDIKAN KARAKTER NOVEL LAUT BERCERITA KARYA LEILA S. CHUDORI SERTA RELEVANSINYA SEBAGAI BAHAN AJAR APRESIASI SASTRA DI SMA
Authors: Setiawan Andy Rachmat , Saddhono Kundharu , Suhita Raheni
Published: December 2019

This research used qualitative research approach with psychology literature review. The data of this research came from the text in the novel Laut Bercerita while the data source came from the results of interviews with teachers, students, counseling and guidance’s teachers, and the authors. The data collection techniques using document analysis and interviews. The technique of taking research subjects with purposive sampling. Data validity using theory triangulation and data triangulation. Data analysis techniques using interactive analysis models which include data collection, data reduction activities, display data, and drawing conclusion. The research procedure consists of the preparation stage, the data collection stage, the data selection stage, the stage of conducting data analysis (interactive models), the stage of preparing the research report. The results of this research are 1) the novel Laut Bercerita has the completeness of intrinsic elements consisting of 7 elements, 2) the inner conflict in the main character can be understood through Sigmund Freud's theory (Id, Ego, Superego) that is able to be influenced by factors within the character itself or external factors of the people around him, 3) there are 12 values of character education contained in the novel Laut Bercerita, and 4) the novel Laut Bercerita is relevant when used as a teaching material for literary appreciation in SMA Negeri 5 Surakarta and SMA Muhammadiyah 1 Surakarta because it contains a lot of  positive values that can be realized for students in real life.
Length-weight relationship and condition factor of Gudusia chapra (Hamilton, 1822) from Panchet Reservoir, Jharkhand, India
Authors: Kumari Suman , Sandhya K.M. , Karnatak Gunjan , - Lianthuamluaia. , Sarkar U.K. , Panda D. , Mishal P.
Published: December 2019

This study provides length-weight relationship and condition factor (K) of Indian river shad Gudusia chapra (Hamilton, 1822) collected from Panchet Reservoir, Jharkhand, India during October 2014 to September 2015 using gillnets and drag nets. About 342 specimens (153 male and 189 female) of length range 4.7 to 15.1cm were collected. Length-weight relationship for male and female were calculated separately. The difference between the sexes was insignificant (independent t-test, p>0.05). The pooled length-weight regression equation was estimated as log W=-1.863+2.855 log L (r=0.983) which indicated negative allometric growth. The value of condition factor (Kn value) was observed to be nearer to or greater than one in both sexes, indicative of the general wellbeing of the fish in the Panchet Reservoir. This is the first report on length-weight relationship parameters of G. chapra from an Indian reservoir.
Effect of extended period of boiling on allergic protein of flower tail shrimp Metapenaeus dobsoni
Authors: Laly S.J. , Sankar T.V. , Panda Satyen Kumar
Published: December 2019

Consumption of shellfishes is a major cause of allergic reaction and IgE mediated hypersensitivity is an increasing health issue in sensitive individuals. Thermal stability of allergen of the flower tail shrimp Metapenaeus dobsoni was evaluated during extended period of boiling for immunoreactivity. Shrimp extracts boiled for 5 to 25 min showed increased IgE reactivity of 0.251 to 0.268 at absorbance of 490 nm in comparison to raw extract. Trpomyosin of 37 KDa was observed to withstand longer periods of boiling and found to retain IgE binding activity. Thermal treatment by means of boiling for longer duration increased the recognition of this major allergen in individuals with shrimp sensitivity. Hence, tropomyosin subjected to longer period of boiling could be used in facilitating shrimp allergy diagnosis and for detection of allergen in processed food products.