bluuebunny/crossref_metadata_2025 huggingface dataset:
Medical help-seeking intentions among patients with early Alzheimer’s disease
Authors: Villarejo-Galende Alberto , García-Arcelay Elena , Piñol-Ripoll Gerard , del Olmo-Rodríguez Antonio , Viñuela Félix , Boada Mercè , Franco-Macías Emilio , Ibañez de la Peña Almudena , Riverol Mario , Puig-Pijoan Albert , Abizanda-Soler Pedro , Arroyo Rafael , Baquero-Toledo Miquel , Feria-Vilar Inmaculada , Balasa Mircea , Berbel Ángel , Rodríguez-Rodríguez Eloy , Vieira-Campos Alba , Garcia-Ribas Guillermo , Rodrigo-Herrero Silvia , Lleó Alberto , Maurino Jorge
Published: December 2023

Background

Limited information is available on the active process of seeking medical help in patients with Alzheimer’s disease (AD) at early stages. The aim of this study was to assess the phenomenon of medical help-seeking in early AD and to identify associated factors.

Methods

A multicenter, non-interventional study was conducted including patients of 50–90 years of age with prodromal or mild AD (National Institute on Aging/Alzheimer’s Association criteria), a Mini-Mental State Examination (MMSE) score ≥ 22, and a Clinical Dementia Rating-Global score (CDR-GS) of 0.5–1.0. A multivariate logistic regression analysis was conducted.

Results

A total of 149 patients were included. Mean age (SD) was 72.3 (7.0) years, 50.3% were female, and 87.2% had a CDR-GS score of 0.5. Mean disease duration was 1.4 (1.8) years. Ninety-four (63.1%) patients sought medical help, mostly from neurologists. Patients with help-seeking intentions were mostly female (60.6%) with a CDR-GS score of 0.5 (91.5%) and had a greater awareness of diagnosis, poorer quality of life, more depressive symptoms, and a more severe perception of their condition than their counterparts. Lack of help-seeking intentions was associated with male sex (p = 0.003), fewer years of education (p = 0.005), a low awareness of diagnosis (p = 0.005), and a low emotional consequence of the condition (p = 0.016).

Conclusion

Understanding the phenomenon of active medical help-seeking may facilitate the design of specific strategies to improve the detection of cognitive impairment, especially in patients with a lower level of educational attainment and poor awareness of their condition.
How do the non-cognitive skills affect retirees’ reemployment? Evidence from China
Authors: Jia Haiyan , Sai Xiaoyu , Si Hongyun , Wang Jinming
Published: December 2023

Introduction

Promoting the reemployment of retirees is important to effectively recognize the capacity of older adults and to help governments cope with an aging global population. Existing research on the factors that impact reemployment has mainly focused on the role of traditional forms of human capital, like education and experience, while ignoring non-cognitive skills.

Methods

Based on 3,693 samples, this study examines the impact of non-cognitive skills on the reemployment of Chinese retirees using the Logit model through the lens of human capital theory.

Results

The results show that non-cognitive skills incentivize retirees to seek reemployment. The incentive effect is greater for retirees who are male, live in a rural household, and are of lower age and education level. Further, the mediation effect model reveals the mediating role of social capital between non-cognitive skills and the reemployment of retirees. Social capital is important to the promotion of retiree reemployment.

Discussion

This study ultimately sheds light on the relationship between non-cognitive skills and the reemployment of retirees. Findings will help improve governments’ understandings of non-cognitive skills so that they may develop better policies on retiree reemployment.
Costs and resource needs for primary health care in Ethiopia: evidence to inform planning and budgeting for universal health coverage
Authors: Alebachew Abebe , Abdella Engida , Abera Samuel , Dessie Ermias , Mesele Tesfaye , Mitiku Workie , Muñoz Rodrigo , Opuni Marjorie , Teplitskaya Lyubov , Walker Damian G. , Gilmartin Colin
Published: December 2023

Introduction

The Government of Ethiopia (GoE) has made significant progress in expanding access to primary health care (PHC) over the past 15 years. However, achieving national PHC targets for universal health coverage will require a significant increase in PHC financing. The purpose of this study was to generate cost evidence and provide recommendations to improve PHC efficiency.

Methods

We used the open access Primary Health Care Costing, Analysis, and Planning (PHC-CAP) Tool to estimate actual and normative recurrent PHC costs in nine Ethiopian regions. The findings on actual costs were based on primary data collected in 2018/19 from a sample of 20 health posts, 25 health centers, and eight primary hospitals. Three different extrapolation methods were used to estimate actual costs in the nine sampled regions. Normative costs were calculated based on standard treatment protocols (STPs), the population in need of the PHC services included in the Essential Health Services Package (EHSP) as per the targets outlined in the Health Sector Transformation Plan II (HSTP II), and the associated costs. PHC resource gaps were estimated by comparing actual cost estimates to normative costs.

Results

On average, the total cost of PHC in the sampled facilities was US$ 11,532 (range: US$ 934–40,746) in health posts, US$ 254,340 (range: US$ 68,860–832,647) in health centers, and US$ 634,354 (range: US$ 505,208–970,720) in primary hospitals. The average actual PHC cost per capita in the nine sampled regions was US$ 4.7, US$ 15.0, or US$ 20.2 depending on the estimation method used. When compared to the normative cost of US$ 38.5 per capita, all these estimates of actual PHC expenditures were significantly lower, indicating a shortfall in the funding required to deliver an expanded package of high-quality services to a larger population in line with GoE targets.

Discussion

The study findings underscore the need for increased mobilization of PHC resources and identify opportunities to improve the efficiency of PHC services to meet the GoE’s PHC targets. The data from this study can be a critical input for ongoing PHC financing reforms undertaken by the GoE including transitioning woreda-level planning from input-based to program-based budgeting, revising community-based health insurance (CBHI) packages, reviewing exempted services, and implementing strategic purchasing approaches such as capitation and performance-based financing.
Acculturation and self-rated health among Chinese and Korean immigrants aged 49 to 75
Authors: Ryu Soomin , Morey Brittany N. , Shi Yuxi , Lee Sunmin
Published: December 2023

Background

Given the rapidly growing Asian populations in the U.S. due to immigration, and the aging demographic of Asian immigrants, it is crucial to understand how acculturation shapes health among older adult Asian immigrants. We study the relationship between acculturation and self-rated health (SRH) and moderating roles of age and Asian subgroup.

Methods

Our cross-sectional study consisted of 200 Chinese and 200 Korean immigrants aged 49–75 living in Baltimore–Washington DC metropolitan area, who were recruited from primary care physicians' clinics in Maryland and Northern Virginia. The participants completed the survey either in-person or by phone in their preferred language. Multidimensional proxies were used to measure acculturation: years living in the U.S. (≥23 years, <23 years), English proficiency (fluently/well, so-so, poorly/not at all), and ethnic identity (very Asian, mostly Asian, bicultural/westernized). SRH was measured using the question “How would you rate your general health?” (excellent/very good/good, fair/poor). Poisson regression models with robust error variance examined associations between acculturation and SRH, accounting for socioeconomic and health insurance factors.

Results

Speaking English so-so and fluently/well had 0.73 (95% confidence intervals (CI): 0.55–0.97) and 0.51 (95% CI: 0.30–0.87) times the prevalence of having fair or poor SRH compared to speaking English poorly/not at all, respectively. The magnitudes and statistical significance of these associations were stronger among Chinese participants than Korean participants. Moreover, individuals who self-identified as bicultural/westernized had 0.63 times the prevalence of having fair or poor SRH (95% CI: 0.43–0.92) as those who self-identified as very Asian. The association was more pronounced among older participants (≥58) compared to younger participants (<58).

Conclusion

Further research should identify the possible mechanisms linking acculturation with health to find effective strategies to enhance health among aging Asian immigrant populations.
Knowledge, attitude, and practice of exclusive breastfeeding among mothers of childbearing age
Authors: Sabo Abdulwali , Abba Justina , Sunusi Usman Usman , Musa Saulawa Ibrahim , Alzoubi Majdi M. , Al-Mugheed Khalid , Alsenany Samira Ahmed , Farghaly Abdelaliem Sally Mohammed
Published: December 2023

Background

The American Academy of Pediatrics and the World Health Organization recommend exclusive breastfeeding (EBF) for up to 6 months. Despite the importance of breast milk, EBF is far less prevalent in Nigeria than is recommended for developing countries. Worse still, the odds of EBF practice are very low in rural communities. Hence, the aim of this study was to assess the knowledge, attitude, and practice of EBF as well as identify the factors associated with EBF practice among mothers of childbearing age in Chamo town, Jigawa State, Nigeria.

Methods

The study is a cross-sectional design using a questionnaire to assess the required information. The methodology involved the use of simple random sampling to select mothers of reproductive age from Chamo town, which is a rural community located in Jigawa State, Nigeria. A semi-structured questionnaire was used to assess the mother’s knowledge, attitude, and practices regarding EBF. Simple and multiple logistic regression analyses were performed to determine the factors associated with the practice of EBF.

Results

A total of 400 mothers between the ages of 18 and 41 took part in the study. More than half of the participants (57.8%) were between the ages of 26 and 33 and had a primary level of education (30.5%). Only 26.8% of the respondents practice EBF. Those with a tertiary education (AOR = 10.00,p< 0.001), civil servants (AOR = 12.51,p< 0.001), those aware of EBF (AOR = 3.65,p= 0.002), those with correct EBF knowledge (AOR = 4.61,p< 0.001), those with a positive attitude toward EBF demand (AOR = 0.51,p= 0.050), and those who received encouragement from their community (AOR = 9.87,p< 0.001) were more likely to practice EBF.

Conclusion

The findings of the study revealed that the majority of the respondents’ knowledge, attitude, and practice of EBF were minimal. This shows the need to step up efforts to educate mothers about the advantages of EBF for both their own health and that of their children while they are in the hospital recovering from childbirth.
Comprehensive assessment of occupational exposure to microbial contamination in waste sorting facilities from Norway
Authors: Viegas Carla , Eriksen Elke , Gomes Bianca , Dias Marta , Cervantes Renata , Pena Pedro , Carolino Elisabete , Twarużek Magdalena , Caetano Liliana Aranha , Viegas Susana , Graff Pål , Afanou Anani Komlavi , Straumfors Anne
Published: December 2023

Introduction

It is of upmost importance to contribute to fill the knowledge gap concerning the characterization of the occupational exposure to microbial agents in the waste sorting setting (automated and manual sorting).

Methods

This study intends to apply a comprehensive field sampling and laboratory protocol (culture based-methods and molecular tools), assess fungal azole resistance, as well as to elucidate on potential exposure related health effects (cytotoxicity analyses). Skin-biota samples (eSwabs) were performed on workers and controls to identify other exposure routes.

Results

In personal filter samples the guidelines in one automated industry surpassed the guidelines for fungi. Seasonal influence on viable microbial contamination including fungi with reduced susceptibility to the tested azoles was observed, besides the observed reduced susceptibility of pathogens of critical priority (Mucorales and Fusarium sp.). Aspergillus sections with potential toxigenic effect and with clinical relevance were also detected in all the sampling methods.

Discussion

The results regarding skin-biota in both controls´ and workers´ hands claim attention for the possible exposure due to hand to face/mouth contact. This study allowed concluding that working in automated and manual waste sorting plants imply high exposure to microbial agents.
Anonymizing at-home fitness: enhancing privacy and motivation with virtual reality and try-on
Authors: Yoon Kang-Il , Jeong Tae-Soo , Kim Seung-Chan , Lim Soo-Chul
Published: December 2023

Introduction

This study aimed to address privacy concerns associated with video conferencing tools used in home-based exercise training. To that end, a method that could anonymize participants' appearances and exercise environments during at-home fitness sessions was proposed.

Methods

This method combines virtual reality for 3-D human-model rendering using key-points tracking with a virtual try-on system enhanced by UV mapping and instance segmentation. To validate the proposed method, we conducted a user study by recruiting participants to assess effectiveness of virtual reality and virtual try-on in terms of privacy protection, self-confidence, and coaching satisfaction.

Results

Experimental results demonstrated the effectiveness and improved user experience of using virtual reality or virtual try-on in remote fitness, particularly in enhancing privacy protection and self-confidence with statistical significance. However, no significant differences were noted in coaching satisfaction.

Discussion

These findings confirmed the efficacy of our proposed approach. We believe that the proposed approach can significantly contribute to the future of remote fitness training, offering a more secure and engaging environment for users, thereby potentially increasing adherence to fitness regimens and overall physical wellbeing.
R-loops in normal and malignant hematopoiesis
Authors: Hirayama Mayumi , Shinriki Satoru , Matsui Hirotaka
Published: December 2023

An R-loop is a nucleic acid structure consisting of a DNA : RNA hybrid and single-stranded DNA. It is formed physiologically in normal cells and is involved in transcription, replication, and gene rearrangement; in particular, it has multiple roles including in mitochondrial DNA replication and class switch recombination of immunoglobulin genes in B cells. However, accumulating evidence indicates aberrant R-loop formation in various malignancies, including hematopoietic neoplasms. The accumulation of such inappropriate R-loops can cause conflicts between transcription and DNA replication. This exacerbates genomic instability through the generation of DNA replication stress, that, in turn, leads to cellular phenotypic changes and disease progression. When RNAs are synthesized during transcription they hybridize with template DNA in cis, giving rise to R-loops. In addition, it was recently revealed that noncoding RNAs also form R-loops when bound to genomic DNA in trans. Together with such observations, new roles for the R-loop in disease development have been proposed. The relationship between inflammation and the R-loop has also attracted much attention. In this review, we will focus on the mechanisms of R-loop formation in various hematopoietic neoplasms and introduce the important findings from recent studies. Therapeutic concepts for targeting R-loop accumulation in hematopoietic neoplasms will also be discussed.
Quantum technology for development framework as a tool for science diplomacy
Authors: Mafu Mhlambululi , Senekane Makhamisa
Published: December 2023

The state-of-the-art quantum technologies leverage the unique principles of quantum mechanics, which include quantization, uncertainty principle, interference, entanglement and decoherence, to produce useful devices and scientific advancements not possible with classical technologies. As a result, quantum technologies, in particular, offer specific advantages that make communications networks secure and unbreakable and devices with unprecedented levels of accuracy, responsiveness, reliability, scalability and efficiency than classical emerging technologies. These capabilities can contribute significantly to addressing energy, agriculture, climate change, national security, healthcare, education and economic growth challenges. Unfortunately, these developments in these areas have not been evenly distributed between the Global North and the Global South, inadvertently creating a societal and economic gap. Closing this gap is critical to creating a more inclusive and sustainable future for all, thus delivering key sustainable goals. Therefore, to close this gap, this article proposes a quantum diplomacy framework as a means to deliver science diplomacy. Moreover, we discuss how emerging quantum technologies could profoundly impact all 17 United Nations Sustainable Development Goals. We consider this work a timely and vital intervention to prevent the gap from increasing.
Social robotics for children: an investigation of manufacturers’ claims
Authors: Dosso Jill A. , Riminchan Anna , Robillard Julie M.
Published: December 2023

As the market for commercial children’s social robots grows, manufacturers’ claims around the functionality and outcomes of their products have the potential to impact consumer purchasing decisions. In this work, we qualitatively and quantitatively assess the content and scientific support for claims about social robots for children made on manufacturers’ websites. A sample of 21 robot websites was obtained using location-independent keyword searches on Google, Yahoo, and Bing from April to July 2021. All claims made on manufacturers’ websites about robot functionality and outcomes (n = 653 statements) were subjected to content analysis, and the quality of evidence for these claims was evaluated using a validated quality evaluation tool. Social robot manufacturers made clear claims about the impact of their products in the areas of interaction, education, emotion, and adaptivity. Claims tended to focus on the child rather than the parent or other users. Robots were primarily described in the context of interactive, educational, and emotional uses, rather than being for health, safety, or security. The quality of the information used to support these claims was highly variable and at times potentially misleading. Many websites used language implying that robots had interior thoughts and experiences; for example, that they would love the child. This study provides insight into the content and quality of parent-facing manufacturer claims regarding commercial social robots for children.
Personal and community values behind sustainable food consumption: a meta-ethnography
Authors: Lamarque Muriel , Tomé-Martín Pedro , Moro-Gutiérrez Lourdes
Published: December 2023

Various aspects of sustainable food consumption have been studied within the Social Sciences in the last years. Specifically, the analysis of motivations and determinants behind alternative economic practices has gained prominence in disciplines such as Sociology, Psychology, Economics and Marketing, seeking to understand, measure and calculate consumers’ decision-making processes and actions through the application of cognitive theories and qualitative predictive models. Anthropology—more specifically through the practice of ethnography—, has also made significant contributions, mostly toward the analysis and description of contemporary cooperative experiences, both in rural and urban settings. However, within this field, few studies have focused on the underlying values, as well as the symbolic, emotional/affective, and identity-based incentives that exist behind the participation in sustainable, pro-environmental, organic, and fair-trade food initiatives. This kind of perspective might help in comprehending how different people or social groups conceptualize their habits and link them to certain representations or beliefs. At the same time, it can provide information about the way in which action-related values appear in discourse and become embodied, whether they are uniform, conflicting, precede practice or emerge as a post-personal reflection of those involved. Through a review, synthesis, and analysis of qualitative literature—meta-ethnography—this paper seeks to present an overview of available academic work on the social, political, personal, material, and ethical reasons associated with partaking in alternative food networks. The findings will be analyzed and discussed in relation to a constructivist perspective, as well as debates around identity, social distinction, and gender.
Ameliorated chest drain wound closure in patients undergoing uniportal thoracoscopic pulmonary resection
Authors: Chou Ping-Ruey , Kao Chieh-Ni , Lo Yu-Ting , Chuang Che-Yu , Liu Yu-Wei
Published: December 2023

Background

Although uniportal video-assisted thoracoscopic surgery (VATS) has been performed for a wide array of thoracic diseases, unsightliness and poor wound healing often occur, particularly when a chest drain is placed postoperatively. Different chest drain wound closure (CWC) methods have been introduced with the benefits of cosmesis and patient satisfaction. We aimed to describe our improved CWC technique in this setting and assess its efficacy.

Methods

A total of consecutive 334 patients undergoing uniportal VATS pulmonary resection with single chest drain placement were investigated from 2016 to 2021. The techniques for CWC were classified into the conventional method (35 patients, group A), continuous suture with removal-free stitches (122 patients, group B), and continuous suture with removal-free barbed suture plus topical skin adhesives (177 patients, group C). Perioperative data and complications related to CWC were analyzed.

Results

Group C had a significantly shorter operative time, postoperative hospital stay, and chest tube days than groups A and B (all p < 0.01). In terms of chest tube-related complications, there were no statistically significant differences in post-removal pneumothorax, subcutaneous emphysema, incisional effusion leakage, wound dehiscence, or infection. Overall, significant differences in scar scale scores were observed between the groups, where the ameliorated group C was superior to the conventional group A (p < 0.01).

Conclusion

The improved CWC technique using continuous sutures with removal-free barbed sutures and topical skin adhesives is simple, safe, and effective. This may be a favorable CWC strategy when performing uniportal VATS, with enhanced patient satisfaction.
Can tree leaves be used as an alternative source of synthetic antioxidants? Use of jujube leaf extract in laying hens
Authors: Kılınç Gözde
Published: December 2023

This study was carried out to determine the antioxidant activity of jujube (Zizyphus jujuba Mill.) leaf extract (JLE) and to evaluate the effects of its use as an extract in laying hen diet (Nick Brown; 32 weeks old) on performance, egg quality and lipid peroxidation. A total of 4 groups (24 replicates/group), one control (JLE-0) and three experimental groups (JLE-1, JLE-2, JLE-3), were formed and the hens were individually distributed in cages. The groups were fed with 0, 45, 90 and 135 mg/kg extract, respectively. The total phenolic content of the extract was 118.60 g gallic acid aquivalent/kg (GAE/kg) and the IC50 value was determined as 332.01 as a result of the DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging activity assay. Performance parameters except for feed conversion ratio (FCR) were not affected by the extract in the diet. Compared to the control group, FCR decreased (p < 0.05) and feed conversion improved in all experimental groups. The linear effect was significant for egg production (EP) (p < 0.05). Furthermore, egg quality parameters except for the albumen index (AI), Haugh unit (HU), shell weight (SW), and shell thickness (ST) were not affected by the extract in the diet. The highest AI and HU were in the JLE-2 group (p < 0.05). Besides, SW was found to increase in all experimental groups (p < 0.001). The highest ST was in JLE-1 (p < 0.001). The addition of the extract was found to slow down lipid oxidation by decreasing Thiobarbituric Acid Reactive substances (TBARs) levels on days 0 and 28 (p < 0.05). In conclusion, JLE can be used as a natural extract in laying hen diets.
Effects of Macro-Pitting Fault on Dynamic Characteristics of Planetary Gear Train Considering Surface Roughness
Authors: Li Rong , Xiong Xin , Ma Jun , Zou Mengting
Published: December 2023

The planetary gearbox plays a vital role in a wide range of mechanical power transmission systems, including high-speed trains, wind turbines, vehicles, and aircraft. At the same time, the planetary gear train inside the gearbox is regarded as the most susceptible to failure in the entire transmission system. To analyze the influence of surface roughness on the dynamic characteristics of the planetary gear train, a dynamic modeling method based on fractal theory is proposed. Firstly, the tooth surface contact model was established based on the W-M fractal function, and the time-varying mesh stiffness (TVMS) of the planetary gear train was calculated under healthy and tooth macro-pitting. Then, the lumped-parameter method is introduced to construct a planetary gear train translation-torsion dynamic model that comprehensively considers TVMS and tooth backlash. The vibration acceleration signals of the planetary gear train under different macro-pitting states and surface roughness are simulated and calculated, allowing a quantificative analysis of the influence of surface roughness on system vibration response. Finally, the correctness of the model for the planetary gear train is verified by experiments. The results show that compared with the planetary gear train modeling method based on Hertz contact theory, the root mean squared error of the vibration signal of this work under a macro-pitting fault state is reduced by 8.7%, further improving the reliability of the model.
Comparison of the Intestinal Bacterial Communities between Captive and Semi-Free-Range Red-Crowned Cranes (Grus japonensis) before Reintroduction in Zhalong National Nature Reserve, China
Authors: Wu Yining , Wang Huan , Gao Zhongyan , Wang He , Zou Hongfei
Published: December 2023

The wild populations of red-crowned cranes (Grus japonensis) in west China are gradually decreasing, necessitating the optimization of reintroduction measures. This study used 16S rRNA high-throughput sequencing technology to compare the gut microbiota communities of cranes living in two modes (captive and semi-free-range) before their reintroduction in Zhalong National Nature Reserve, Heilongjiang Province, China. The results showed that Proteobacteria (74.39%) and Firmicutes (25.29%) were the dominant gut bacterial phyla inhabiting these cranes. Significant differences were found in the gut microbiota community composition between semi-free-range and captive cranes (p < 0.01). Psychrobacter, Sporosarcina, and Lactococcus were significantly enriched in captive cranes (p < 0.05), while Pseudomonadaceae_Pseudomonas, Pantoea, Lysobacter, and Enterobacteriaceae_Pseudomonas were more abundant in semi-free-range cranes (p < 0.05). The functions and community structure of gut microbiota were affected by feeding patterns (p < 0.05). The metabolic pathways of ethylbenzene degradation, PPAR signaling pathway, betalain biosynthesis, systemic lupus erythematosus, and shigellosis were up-regulated in semi-free-range cranes (p < 0.05).
Full-Length Transcriptome and Gene Expression Analysis of Different Ovis aries Adipose Tissues Reveals Transcript Variants Involved in Lipid Biosynthesis
Authors: An Lixia , Pan Yangyang , Yuan Mengjiao , Wen Zhonghao , Qiao Liying , Wang Weiwei , Liu Jianhua , Li Baojun , Liu Wenzhong
Published: December 2023

Sheep have historically been bred globally as a vital food source. To explore the transcriptome of adipose tissue and investigate key genes regulating adipose metabolism in sheep, adipose tissue samples were obtained from F1 Dorper × Hu sheep. High-throughput sequencing libraries for second- and third-generation sequencing were constructed using extracted total RNA. Functional annotation of differentially expressed genes and isoforms facilitated the identification of key regulatory genes and isoforms associated with sheep fat metabolism. SMRT-seq generated 919,259 high-accuracy cDNA sequences after filtering. Full-length sequences were corrected using RNA-seq sequences, and 699,680 high-quality full-length non-chimeric (FLNC) reads were obtained. Upon evaluating the ratio of total lengths based on FLNC sequencing, it was determined that 36,909 out of 56,316 multiple-exon isoforms met the criteria for full-length status. This indicates the identification of 330,375 full-length FLNC transcripts among the 370,114 multiple-exon FLNC transcripts. By comparing the reference genomes, 60,276 loci and 111,302 isoforms were identified. In addition, 43,423 new genes and 44,563 new isoforms were identified. The results identified 185 (3198), 394 (3592), and 83 (3286) differentially expressed genes (transcripts) between tail and subcutaneous, tail and visceral, and subcutaneous and visceral adipose tissues, respectively. Functional annotation and pathway analysis revealed the following observations. (1) Among the differentially expressed genes (DEGs) of TF and SF tissues, the downregulation of ACADL, ACSL6, and NC_056060.1.2536 was observed in SF, while FFAR4 exhibited upregulation. (2) Among the DEGs of TF and VF tissues, expressions of ACADL, ACSL6, COL1A1, COL1A2, and SCD were downregulated in VF, with upregulation of FFAR4. (3) Among SF and VF expressions of COL1A1, COL1A2, and NC_056060.1.2536 were downregulated in VF. Specific differentially expressed genes (ACADL, ACSL6, COL1A1, COL1A2, FFAR4, NC_056060.1.2536, and SCD) and transcripts (NC_056066.1.1866.16 and NC_056066.1.1866.22) were identified as relevant to fat metabolism. These results provide a dataset for further verification of the regulatory pathway associated with fat metabolism in sheep.
How Useful Are Existing Protocols in the Quick Assessment of the Welfare of Semi-Feral Horses? Pilot Study on Konik Polski Horses Living in the Forest Sanctuary
Authors: Górecka-Bruzda Aleksandra , Siemieniuch Marta , Lansade Léa , Stanley Christina R.
Published: December 2023

Scientifically validated and standardised methods for the evaluation of the welfare of free-living horses are urgently needed by both the owners and managers of these populations and those responsible for implementing national welfare legislation. The aim of the study was to test the feasibility and usefulness of two welfare protocols that could be applied to semi-feral populations: a prototype of welfare assessment template (WAT) for Carneddau semi-feral ponies and the IFCE/INRAE Horse Welfare Protocol. Additionally, the body condition scale designed by Henneke (BCS-H) was employed. The study took place in July/August 2022 and April 2023 to evaluate the welfare of a pilot population of nineteen semi-feral Konik polski horses. The horses scored high or satisfactory under indicators across both protocols; only body condition scores were significantly lower in early spring (BCS-WAT: 1.11 ± 0.57; BCS-H: 3.84 ± 1.17) than in the summer (BCS-WAT: 1.58 ± 0.61; BCS-H: 5.63 ± 1.01). Our study confirmed the feasibility of utilising most of the WAT and IFCE/INRAE welfare indicators in semi-feral horses. Some adaptations, such as considering validation of scales, positive welfare indicators and animals’ free-choice of conditions, have been suggested for future in-field application.
Ex Vivo Comparison of the Diagnostic Performance of Two-Dimensional and Three-Dimensional Three-Tesla Magnetic Resonance Imaging Sequences in Depicting Normal Articular Cartilage in Equine Stifle Cadavers
Authors: Seidler Annika , Aßmann Anton , Torgerson Paul R. , Sánchez-Andrade José Suárez , Bischofberger Andrea
Published: December 2023

The objective of this study was to compare articular cartilage thickness observed in the different 2D and 3D sequences to the cartilage thickness of the equine stifle in cadavers to determine the accuracy of each sequence. The study was conducted as a blinded laboratory study using seven equine stifle specimens. The 2D (T2W TSE) and 3D (3D VIEW T2W HR, T2 3D mFFE, T1W VISTA SPAIR, 3D PDW SPAIR) 3-tesla MRI sequences of each stifle were obtained. Cartilage thickness was measured at 30 locations on MRI and on gross pathology. Thickness measurements were compared using a Bland–Altman plot and post hoc analysis tests. The 3D sequences were found to be generally more accurate than the 2D sequence (p < 0.001). The smallest difference to macroscopic measurements was observed in the 3D VIEW T2W HR and T1W VISTA SPAIR sequences with no statistical difference between each other. Knowing the accuracy of different sequences will improve the evaluation of equine cartilage and the early detection of cartilage pathologies. This would promote MRI as a noninvasive imaging modality for horses suffering from stifle lameness with no findings in conventional imaging methods. Furthermore, since 3D sequences seem to have better accuracy in depicting cartilage, they may replace 2D sequences, thereby shortening scanning times.
Valorization of Olive Leaf Polyphenols by Green Extraction and Selective Adsorption on Biochar Derived from Grapevine Pruning Residues
Authors: Prelac Melissa , Major Nikola , Cvitan Danko , Anđelini Dominik , Repajić Maja , Ćurko Josip , Kovačević Tvrtko Karlo , Goreta Ban Smiljana , Užila Zoran , Ban Dean , Palčić Igor
Published: December 2023

Given today’s increasingly intensive agriculture, one key problem area considers the valorization and reuse of wastes from food and agricultural production with minimal impact on the environment. Due to its physicochemical characteristics, biochar (BC) derived from grapevine pruning residue has shown considerable potential for use as an adsorbent. High-value phytochemicals found in abundance in the olive leaf (OL) can be employed in many different industrial sectors. The potential application of BC in the removal of specific polyphenolic components from OL extracts has been investigated in the present study. Water, as the most available and greenest of solvents, was investigated as to its use in the extraction of polyphenols, which was carried out by comparing maceration, ultrasound-assisted extraction, and microwave-assisted extraction, considering different temperatures and solid-to-liquid (s/l) ratios. The BC adsorption capacity of selected polyphenols was fitted with both the Langmuir and Freundlich isotherm models. The Freundlich model fitted better relative to OL polyphenols adsorption. Oleuropein was the most abundant compound identified in the extracts, obtaining the highest Kf value (20.4 (mg/g) × (L/g)n) and R2 coefficient (0.9715) in the adsorption on the biochar’s surface. The optimum conditions in the dosage experiment suggest the use of 0.5 g of BC using 3 g/L extracts, with an exception for oleuropein and hydroxytyrosol, for which the highest biochar dose (2.5 g) performed better. Considering the compounds’ concentrations and the BC dose, BC from grapevine pruning residues demonstrated a potential use in the uptake of specific polyphenols from olive leaves, making it a promising adsorbent for such applications.
Relationship between Aspartame-Induced Cerebral Cortex Injury and Oxidative Stress, Inflammation, Mitochondrial Dysfunction, and Apoptosis in Sprague Dawley Rats
Authors: U-pathi Jureeporn , Yeh Yen-Chia , Chen Chia-Wen , Owaga Eddy E. , Hsieh Rong-Hong
Published: December 2023

There are emerging concerns about the potential cerebral cortex injury from aspartame due to the accumulation of the various neurotoxic metabolic components in the central nervous system after long-term dietary exposure. The aim of this study was to evaluate the effect of oral aspartame consumption on cerebral cortex injury in the rat brain, and further evaluate the various underlying molecular mechanisms, with a special focus on oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis pathways. Sprague Dawley rats (nineteen, female) were randomly sub-divided into three groups: (i) normal diet with vehicle: control group (five rats), (ii) low dose of aspartame group (LA): seven rats received 30 mg/kg body weight (bw) daily doses of aspartame, (iii) high dose of aspartame group (HA): seven rats received 60 mg/kg bw daily doses of aspartame. After 8 weeks, the LA and HA groups showed lower expression levels of brain-derived neurotrophic factor (BDNF), antioxidant enzyme activity (SOD2, CAT), antioxidant marker (Nrf2), inflammatory response (IκB), mitochondrial biogenesis (Sirt1, PGC1α, Nrf1, TFAM), mitochondrial DNA (mtDNA) copy number, and apoptosis-related proteins (Bax, Caspase-3) expressions. Aspartame administration also elevated oxidative stress levels (Malondialdehyde, MDA), 8-hydroxy-2-deoxy guanosine (8-OHdG), PGE2 and COX-2 expressions, pro-inflammatory cytokines (TNFα, IL6, IL1β), antioxidant marker expression (Keap1), inflammatory responses (iNOS, NFκB), and glial fibrillary acidic protein (GFAP) levels in the cerebral cortex of the rats, thereby contributing to the reduced survival of pyramidal cells and astrocyte glial cells of the cerebral cortex. Therefore, these findings imply that aspartame-induced neurotoxicity in rats’ cerebral cortex could be regulated through four mechanisms: inflammation, enhanced oxidant stress, decreased mitochondrial biogenesis, and apoptosis pathways.
Have Pesticides and Fertilizers Improved Agricultural Development? The Threshold Effect Based on China’s Agricultural Film Usage
Authors: Li Zining , Li Congxin , Wang Liqi
Published: December 2023

The use of agricultural films, pesticides, and fertilizers has increased in China, leading to the degradation of the agricultural ecological environment. Based on the panel data of 30 provinces in China from 2010 to 2021, this paper takes agricultural film usage as the threshold variable to study the impact of pesticide and fertilizer usage on agricultural development. The research results show that a single threshold effect of agricultural film usage is significant. When agricultural film usage is both a core and threshold variable, that is lower than the threshold value, every 1% increase in agricultural film usage will reduce agricultural output value by 0.9862%, and the impact is significant. When agricultural film usage crosses the threshold value, every 1% increase in agricultural film usage will increase agricultural output value by 1.1843%, and the impact is significant. When pesticide usage is the core variable, regardless of whether agricultural film usage is below or above the threshold, pesticide usage promotes agricultural development. Its impact is significant. When fertilizer input is a core variable, regardless of whether agricultural film usage is below or above the threshold, the use of fertilizers hinders agricultural development. The impact of fertilizer input on agricultural development is significant. Through empirical testing, this paper explores the deep relationship between agricultural film usage, pesticide usage and fertilizer input on agricultural development. These can effectively allocate agricultural production factors, transform the mode of agricultural economic growth, achieve the coordinated development of the ecological environment and agricultural economy and provide data support for the formulation of agricultural green development policies.
Loop Closure Detection Based on Compressed ConvNet Features in Dynamic Environments
Authors: Jiang Shuhai , Zhou Zhongkai , Sun Shangjie
Published: December 2023

In dynamic environments, convolutional neural networks (CNNs) often produce image feature maps with significant redundancy due to external factors such as moving objects and occlusions. These feature maps are inadequate as precise image descriptors for similarity measurement, hindering loop closure detection. Addressing this issue, this paper proposes feature compression of convolutional neural network output. The approach is detailed as follows: (1) employing ResNet152 as the backbone feature-extraction network, a Siamese neural network is constructed to enhance the efficiency of feature extraction; (2) utilizing KL transformation to extract principal components from the backbone network’s output, thereby eliminating redundant information; (3) employing the compressed features as input for NetVLAD to construct a spatially informed feature descriptor for similarity measurement. Experimental results demonstrate that, on the New College dataset, the proposed improved method exhibits an approximately 9.98% enhancement in average accuracy compared to the original network. On the City Center dataset, there is an improvement of approximately 2.64%, with an overall increase of about 23.51% in time performance. These findings indicate that the enhanced ResNet152 performs better than the original network in environments with more moving objects and occlusions.
A Method of Enhancing Silk Digital Printing Color Prediction through Pix2Pix GAN-Based Approaches
Authors: Zhu Weijing , Wang Zhe , Li Qizheng , Zhu Chengyan
Published: December 2023

Color prediction and color management for digital printed fabrics remain a challenging task. Accurate prediction of color appearances of digital printed fabrics would enable designers and manufacturers to better fulfill their design requirements and creative visions. We propose a color prediction method for silk digital printing utilizing a Pix2Pix Generative Adversarial Network (GAN) framework. This method aims to generate predicted images that possess the same stylistic and color characteristics as their actual fabrics after production. To develop and validate the method, color data and images are collected and processed from 5252 sets of paired original Pantone TPX color card and actual print sample fabrics. The results of this study demonstrate that the method can predict the colors of silk digital print samples while effectively reproducing the effects of inkjet printing in silk fabrics including silk crepe satin and silk twill. The method exhibits high prediction accuracy to an average CIEDE2000 value of 2.372 for silk crepe satin and 1.846 for silk twill. The findings of this research not only enhance the efficiency and accuracy of color management in fabric digital printing technology but also contribute to the exploration and development of high-fidelity color prediction techniques within the textile industry.
Towards Lunar In-Situ Resource Utilization Based Subtractive Manufacturing
Authors: Seidel André , Teicher Uwe , Ihlenfeldt Steffen , Sauer Konstantin , Morczinek Florian , Dix Martin , Niebergall Rick , Durschang Bernhard , Linke Stefan
Published: December 2023

In recent years, space agencies, such as the National Aeronautics and Space Administration (NASA) and European Space Agency (ESA), have expanded their research activities in the field of manufacturing in space. These measures serve to reduce limitations and costs through fairing size, launch mass capabilities or logistic missions. The objective, in turn, is to develop technologies and processes that enable on-demand manufacturing for long-term space missions and on other celestial bodies. Within these research activities, in-situ resource utilization (ISRU) and recycling are major topics to exploit local resources and save transport capacity and, therefore, costs. On the other hand, it is important to carefully consider which items can be brought and which must be manufactured on the Moon. Consequently, on-demand needs in future space missions are considered regarding frequency, raw material and required manufacturing processes according to investigations by ESA and NASA. In conclusion, manufacturing in space state-of-the-art shows a strong focus on additive processes, primarily considering semicrystalline or amorphous plastics. The subtractive processing of metallic or ceramic materials, in turn, currently represents a research gap. Consequently, an approach for in-situ resource utilization-based subtractive manufacturing in space is presented to supplement the existing processes. The latter uses a high-pressure jet of water, with regolith simulate as abrasive in suspension, being directed at the workpiece, which is moved to separate metal and glass. Proof-of-concept results are presented, including suitable process windows, achieved cutting geometries, as well as the effects of parameter variations on the system technology and consumables used. The focus of the investigations supplements the general requirements for the design of machine tools for space applications with inertial process-specific boundary conditions as a step towards higher technology maturity.
Elastic Resistance and Shoulder Movement Patterns: An Analysis of Reaching Tasks Based on Proprioception
Authors: Shim Gyuseok , Yang Duwon , Cho Woorim , Kim Jihyeon , Ryu Hyangshin , Choi Woong , Kim Jaehyo
Published: December 2023

This study departs from the conventional research on horizontal plane reach movements by examining human motor control strategies in vertical plane elastic load reach movements conducted without visual feedback. Here, participants performed shoulder presses with elastic resistances at low, moderate, and high intensities without access to visual information about their hand position, relying exclusively on proprioceptive feedback and synchronizing their movements with a metronome set at a 3 s interval. The results revealed consistent performance symmetry across different intensities in terms of the reach speed (p = 0.254–0.736), return speed (p = 0.205–0.882), and movement distance (p = 0.480–0.919). This discovery underscores the human capacity to uphold bilateral symmetry in movement execution when relying solely on proprioception. Furthermore, this study observed an asymmetric velocity profile where the reach duration remained consistent irrespective of the load (1.15 s), whereas the return duration increased with higher loads (1.39 s–1.45 s). These findings suggest that, in the absence of visual feedback, the asymmetric velocity profile does not result from the execution of the action but rather represents a deliberate deceleration post-reach aimed at achieving the target position as generated by the brain’s internal model. These findings hold significant implications for interpreting rehabilitation approaches under settings devoid of visual feedback.
Emerging Trends in Complications Associated with SARS-CoV-2 Infection
Authors: Rosca Elena Cecilia , Cornea Amalia , Simu Mihaela
Published: December 2023

The coronavirus disease 2019 (COVID-19) pandemic has presented a remarkable challenge to global health, sparking a surge in research aimed at understanding the multifaceted impacts of the virus [...]