bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomimetic 3D Models for Investigating the Role of Monocytes and Macrophages in Atherosclerosis
Authors: Garcia-Sabaté Anna , Mohamed Walaa Kamal E. , Sapudom Jiranuwat , Alatoom Aseel , Al Safadi Layla , Teo Jeremy C. M.
Published: September 2020

Atherosclerosis, the inflammation of artery walls due to the accumulation of lipids, is the most common underlying cause for cardiovascular diseases. Monocytes and macrophages are major cells that contribute to the initiation and progression of atherosclerotic plaques. During this process, an accumulation of LDL-laden macrophages (foam cells) and an alteration in the extracellular matrix (ECM) organization leads to a local vessel stiffening. Current in vitro models are carried out onto two-dimensional tissue culture plastic and cannot replicate the relevant microenvironments. To bridge the gap between in vitro and in vivo conditions, we utilized three-dimensional (3D) collagen matrices that allowed us to mimic the ECM stiffening during atherosclerosis by increasing collagen density. First, human monocytic THP-1 cells were embedded into 3D collagen matrices reconstituted at low and high density. Cells were subsequently differentiated into uncommitted macrophages (M0) and further activated into pro- (M1) and anti-inflammatory (M2) phenotypes. In order to mimic atherosclerotic conditions, cells were cultured in the presence of oxidized LDL (oxLDL) and analyzed in terms of oxLDL uptake capability and relevant receptors along with their cytokine secretomes. Although oxLDL uptake and larger lipid size could be observed in macrophages in a matrix dependent manner, monocytes showed higher numbers of oxLDL uptake cells. By analyzing major oxLDL uptake receptors, both monocytes and macrophages expressed lectin-like oxidized low-density lipoprotein receptor-1 (LOX1), while enhanced expression of scavenger receptor CD36 could be observed only in M2. Notably, by analyzing the secretome of macrophages exposed to oxLDL, we demonstrated that the cells could, in fact, secrete adipokines and growth factors in distinct patterns. Besides, oxLDL appeared to up-regulate MHCII expression in all cells, while an up-regulation of CD68, a pan-macrophage marker, was found only in monocytes, suggesting a possible differentiation of monocytes into a pro-inflammatory macrophage. Overall, our work demonstrated that collagen density in the plaque could be one of the major factors driving atherosclerotic progression via modulation of monocyte and macrophages behaviors.
How Different Are the Molecular Mechanisms of Nodal and Distant Metastasis in Luminal A Breast Cancer?
Authors: Lapcik Petr , Pospisilova Anna , Janacova Lucia , Grell Peter , Fabian Pavel , Bouchal Pavel
Published: September 2020

Lymph node status is one of the best prognostic factors in breast cancer, however, its association with distant metastasis is not straightforward. Here we compare molecular mechanisms of nodal and distant metastasis in molecular subtypes of breast cancer, with major focus on luminal A patients. We analyze a new cohort of 706 patients (MMCI_706) as well as an independent cohort of 836 primary tumors with full gene expression information (SUPERTAM_HGU133A). We evaluate the risk of distant metastasis, analyze targetable molecular mechanisms in Gene Set Enrichment Analysis and identify relevant inhibitors. Lymph node positivity is generally associated with NF-κB and Src pathways and is related to high risk (OR: 5.062 and 2.401 in MMCI_706 and SUPERTAM_HGU133A, respectively, p < 0.05) of distant metastasis in luminal A patients. However, a part (≤15%) of lymph node negative tumors at the diagnosis develop the distant metastasis which is related to cell proliferation control and thrombolysis. Distant metastasis of lymph node positive patients is mostly associated with immune response. These pro-metastatic mechanisms further vary in other molecular subtypes. Our data indicate that the management of breast cancer and prevention of distant metastasis requires stratified approach based on targeted strategies.
Genome-Wide Comparison of the Target Genes of the Reactive Oxygen Species and Non-Reactive Oxygen Species Constituents of Cold Atmospheric Plasma in Cancer Cells
Authors: Ji Hwee Won , Kim Heejoo , Kim Hyeon Woo , Yun Sung Hwan , Park Jae Eun , Choi Eun Ha , Kim Sun Jung
Published: September 2020

Cold atmospheric plasma (CAP) can induce cancer cell death. The majority of gene regulation studies have been biased towards reactive oxygen species (ROS) among the physicochemical components of CAP. The current study aimed to systemically determine the distribution of target genes regulated by the ROS and non-ROS constituents of CAP. Genome-wide expression data from a public database, which were obtained after treating U937 leukemia and SK-mel-147 melanoma cells with CAP or H2O2, were analyzed, and gene sets regulated by either or both of them were identified. The results showed 252 and 762 genes in H2O2-treated U937 and SK-mel-147 cells, respectively, and 112 and 843 genes in CAP-treated U937 and SK-mel-147 cells, respectively, with expression changes higher than two-fold. Notably, only four and two genes were regulated by H2O2 and CAP in common, respectively, indicating that non-ROS constituents were responsible for the regulation of the majority of CAP-regulated genes. Experiments using ROS and nitrogen oxide synthase (NOS) inhibitors demonstrated the ROS- and reactive nitrogen species (RNS)-independent regulation of PTGER3 and HSPA6 when U937 cancer cells were treated with CAP. Taken together, this study identified CAP-specific genes regulated by constituents other than ROS or RNS and could contribute to the annotation of the target genes of specific constituents in CAP.
Hormone Involvement in Tissue Development, Physiology and Oncogenesis: A Preface to the Special Issue
Authors: Luparello Claudio
Published: September 2020

Hormones, i [...]
Adding PD-1/PD-L1 Inhibitors to Chemotherapy for the First-Line Treatment of Extensive Stage Small Cell Lung Cancer (SCLC): A Meta-Analysis of Randomized Trials
Authors: Facchinetti Francesco , Di Maio Massimo , Tiseo Marcello
Published: September 2020

Survival outcomes in extensive-stage small cell lung cancer (ES SCLC) are dismal, with median overall survival (OS) less than 12 months. The combination of PD-1/PD-L1 immune checkpoint inhibitors (ICIs) with first-line platinum-etoposide chemotherapy has been recently evaluated in randomized clinical trials. We performed a systematic literature review through PubMed and conference proceedings. Randomized trials evaluating chemotherapy +/− PD-1/PD-L1 ICIs were included in the meta-analysis. Efficacy (OS), activity [progression-free survival (PFS) and objective response rate (ORR)] outcomes and toxicities were analyzed. For selected endpoints, we focused on patients’ subgroups (OS) and on landmark analyses (OS, PFS). Four randomized trials were identified; globally, 1553 patients were randomized to receive chemotherapy +/− PD-1/PD-L1 ICIs. Adding a PD-1/PD-L1 ICI to chemotherapy led to a significant benefit in OS [hazard ratio (HR) 0.76, 95% confidence interval (CI) 0.68–0.85, p < 0.00001), PFS [HR 0.75, 95% CI 0.68–0.84, p < 0.00001] and ORR [odds ratio 1.28, 95% CI 1.04–1.57, p = 0.02]. No unexpected toxicity emerged. At 12, 18, 24 months for OS, and at 12, 18 months for PFS, experimental arms retained significant improvement in event-free rates, with absolute gain of approximately 10% compared with standard treatment. Albeit the magnitude of the benefit is less impacting compared to other settings of immunotherapy, the addition of PD-1/PD-L1 ICIs to chemotherapy in ES SCLC provided significant improvements in survival outcomes with the known toxicity profile. Biomarkers predicting which patients are suitable to derive long-term benefits are eagerly awaited.
Tissue Immune Profile: A Tool to Predict Response to Neoadjuvant Therapy in Triple Negative Breast Cancer
Authors: Cerbelli Bruna , Scagnoli Simone , Mezi Silvia , De Luca Alessandro , Pisegna Simona , Amabile Maria Ida , Roberto Michela , Fortunato Lucio , Costarelli Leopoldo , Pernazza Angelina , Strigari Lidia , Della Rocca Carlo , Marchetti Paolo , d’Amati Giulia , Botticelli Andrea
Published: September 2020

Pathological complete response (pCR) after neoadjuvant chemotherapy (NACT) can predict better survival outcomes in patients with early triple negative breast cancer (TNBC). Tumor infiltrating lymphocytes (TILs), Programmed Death-Ligand 1 (PD-L1), and Cluster of Differentiation 73 (CD73) are immune-related biomarkers that can be evaluated in the tumor microenvironment. We investigated if the contemporary expression of these biomarkers combined in a tissue immune profile (TIP) can predict pCR better than single biomarkers in TNBC. Tumor infiltrating lymphocytes (TILs), CD73 expression by cancer cells (CC), and PD-L1 expression by immune cells (IC) were evaluated on pre-NACT biopsies. We defined TIP positive (TIP+) as the simultaneous presence of TILS ≥ 50%, PD-L1 ≥ 1%, and CD73 ≤ 40%. To consider the effects of all significant variables on the pCR, multivariate analysis was performed. Akaike information criterion (AIC) and Bayesian information criterion (BIC) were used for model selection. We retrospectively analyzed 60 biopsies from patients with TNBC who received standard NACT. Pathological complete response was achieved in 23 patients (38.0%). Twelve (20.0%) cases resulted to be TIP+. The pCR rate was significantly different between TIP+ (91.7%) and TIP− (25.0%) (p < 0.0001). Using a multivariate analysis, TIP was confirmed as an independent predictive factor of pCR (OR 49.7 (6.30–392.4), p < 0.0001). Finally, we compared the efficacy of TIP versus each single biomarker in predicting pCR by AIC and BIC. The combined immune profile is more accurate in predicting pCR (AIC 68.3; BIC 74.5) as compared to single biomarkers. The association between TIP+ and pCR can be proposed as a novel link between immune background and response to chemotherapy in TNBC, highlighting the need to consider an immunological patients’ profile rather than single biomarkers.
Relation between Fish Habitat and the Periodicity of Incremental Lines in the Fossil Otoliths
Authors: Mishima Hiroyuki , Kondo Yasuo , Ohe Fumio , Miake Yasuo , Hayakawa Tohru
Published: September 2020

There are few research reports on the relationship between fish habitats and the periodicity of the fishes’ incremental lines of otolith fossils. The present study examines this relationship through histological and analytical studies on otolith fossils from Nobori Formation, Pliocene, Japan. The specimens were observed and analyzed using light microscopy, polarizing microscopy, Miniscopy, Scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) analysis, electron probe X-ray microanalyzer (EPMA), Raman spectroscopy, and XRD. The otolith crystals were aragonite according to XRD and Raman analysis. The incremental lines contained C, O, and Ca, with Si as a trace element. In the layer between the incremental lines, Si was not detected. The circadian incremental lines were unclear and irregularly observed in both Lobianchia gemellarii and Diaphus gigas. Their behavioral pattern included a diurnal vertical movement. By comparison, for Cetonurus noboriensis, Ventrifossa sp., Sebastes scythropus, and Congriscus megastomus, the circadian incremental lines were evident. The habitat of the fishes that live exclusively on the continental slope is kept constant, and the circadian incremental lines are formed regularly. However, for fishes that spend the day in the deep sea and ascend to the shallow sea at night, the ecosystem, such as seawater temperature and pressure, fluctuates, and the circadian incremental lines become unclear and irregular. The period of the circadian incremental lines of otolith may vary due to differences in the ecosystems.
Investigation of Pinewood Nematodes in Pinus tabuliformis Carr. under Low-Temperature Conditions in Fushun, China
Authors: Pan Long , Cui Rong , Li Yongxia , Feng Yuqian , Zhang Xingyao
Published: September 2020

In recent years, the pinewood nematode has continuously adapted to low-temperature environments and expanded from the South to the North of China. In December 2018, a large area of pinewood nematode was suspected to be harmful to Pinus tabuliformis under natural conditions in Fushun City, Liaoning Province. In order to clarify the low-temperature environment and population characteristics of pinewood nematodes in this new epidemic area, we analyzed the difference in temperature between the inside and outside of P. tabuliformis in low-temperature environments, conducted the morphological and molecular identification of pinewood nematodes in P. tabuliformis, summarized the distribution characteristics of the wintering of pinewood nematodes and explored the population structure of pinewood nematodes under different low-temperature conditions. The results indicated that the diurnal variation of temperature in dead P. tabuliformis was significantly less than the environment temperature. The lowest temperature in P. tabuliformis was 3.2 °C higher than the lowest temperature in the environment in one day; the pathogen of a large area of dead P. tabuliformis in Fushun was pinewood nematode (Bursaphelenchus xylophilus); 84.9% of the average populations of pinewood nematodes were third-stage dispersal juveniles, which mainly gathered in 5 cm within the pupal chamber of Monochamus saltuarius Gebler. At −40 ℃, most of the third-stage dispersal juveniles of pinewood nematode in dead pine can still survive. Our study laid a foundation for the understanding of the low-temperature adaptation mechanism of pinewood nematode and contributed to the monitoring of pine wilt disease in the mid-temperate zone.
Thaumatin-Like Protein (TLP) Gene Family in Barley: Genome-Wide Exploration and Expression Analysis during Germination
Authors: Iqbal Irfan , Tripathi Rajiv Kumar , Wilkins Olivia , Singh Jaswinder
Published: September 2020

Thaumatin-like Proteins (TLPs) are known to play a vital role in plant defense, developmental processes and seed germination. We identified 19 TLP genes from the reference genome of barley and 37, 28 and 35 TLP genes from rice, Brachypodium and sorghum genomes, respectively. Comparative phylogenetic analysis classified the TLP family into nine groups. Localized gene duplications with diverse exon/intron structures contributed to the expansion of the TLP gene family in cereals. Most of the barley TLPs were localized on chromosome 5H. The spatiotemporal expression pattern of HvTLP genes indicated their predominant expression in the embryo, developing grains, root and shoot tissues. Differential expression of HvTLP14, HvTLP17 and HvTLP18 in the malting variety (Morex) over 16–96 h of grain germination revealed their possible role in malting. This study provides a description of the TLP gene family in barley and their possible involvement in seed germination and the malting process.
Association of Low Bone Mass with Decreased Skeletal Muscle Mass: A Cross-Sectional Study of Community-Dwelling Older Women
Authors: Nonaka Koji , Murata Shin , Nakano Hideki , Anami Kunihiko , Shiraiwa Kayoko , Abiko Teppei , Goda Akio , Iwase Hiroaki , Horie Jun
Published: September 2020

This study aimed to investigate the characteristics of skeletal muscle mass, muscle strength, and physical performance among community-dwelling older women. Data were collected from 306 older adults, and the data of 214 older women were included in the final analysis. Participants’ calcaneus bone mass was measured using ultrasonography. Based on their T-scores, participants were divided into the following three groups: normal (T-score > −1), low (−2.5 < T-score ≤ −1), and very low (T-score ≤ −2.5) bone mass. Further, participants’ skeletal muscle mass, muscle strength (grip and knee extension strength), and physical performance [gait speed and timed up and go (TUG)] were measured. Arm skeletal muscle index (SMI, skeletal muscle mass/height2), leg SMI, and appendicular SMI in the very low bone mass group were low compared to those of the low bone mass group (p = 0.034, p = 0.011, and p = 0.009, respectively). Grip and knee extension strength, gait speed, and TUG were not significantly different between the groups. These findings suggest that older women with low bone density had decreased skeletal muscle mass. Therefore, maintaining or improving skeletal muscle mass may prevent low bone mass.
Development of Bionanocomposites Based on Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate)/PolylActide Blends Reinforced with Cloisite 30B
Authors: Lacoste Clément , Gallard Benjamin , Lopez-Cuesta José-Marie , Ipek Kalaoglu-Altan Ozlem , De Clerck Karen
Published: September 2020

In the present study, poly(3-hydroxybuturate-co-3-hydroxyvalerate) (PHBV) and plasticized polylactide acid (PLA) blends were processed by melt extrusion with different weight ratio (up to 20 wt.% of PHBV). Bionanocomposites were obtained through the incorporation of an organomodified montmorillonite (C30B) at 3 wt.%. The main features of the processing and physico-chemical characterization of films and injected samples were assessed and the influence of the components on the chemical, thermal and mechanical properties of the bionanocomposites was investigated. The results indicated that plasticized PLA/PHBV/C30B bionanocomposites present optimal mechanical properties for sanitary applications. Moreover, plasticized PLA/PHBV could lead to finely tuned biomaterials able to form electrospun nanofibers.
Coating Reactions on Vanadium and V-Si-B Alloys during Powder Pack-Cementation
Authors: Hasemann Georg , Harris Chad , Krüger Manja , Perepezko John H.
Published: September 2020

Alloys in the V-Si-B system are a new and promising class of light-weight refractory metal materials for high temperature applications. Presently, the main attention is focused on three-phase alloy compositions that consist of a vanadium solid solution phase and the two intermetallic phases V3Si and V5SiB2. Similar to other refractory metal alloys, a major drawback is the poor oxidation resistance. In this study, initial pack-cementation experiments were performed on commercially available pure vanadium and a three-phase alloy V-9Si-5B to achieve an oxidation protection for this new type of high temperature material. This advance in oxidation resistance now enables the attractive mechanical properties of V-Si-B alloys to be used for high temperature structural applications.
Prediction of Optimum Process Parameters Fabricated by Direct Laser Interference Patterning Based on Central Composite Design
Authors: El-Khoury Mikhael , Voisiat Bogdan , Kunze Tim , Lasagni Andrés Fabián
Published: September 2020

In this study, we report on the optimization of the direct laser interference patterning process by applying the design of experiments approach. The periodic line-like microstructures of a 8.50 µm spatial period were fabricated by a two-beam interference setup with nanosecond laser pulses, varying laser fluence, pulse overlap, and hatch distance. Central composite design with three factors and five levels was implemented to optimize the required number of experiments. The experimental and numerical results show the impact of various structuring process parameters on surface uniformity. The responses measured are the structure height, height error, and waviness of the pattern. An analysis of the microstructures on the patterned surface was conducted by confocal microscopy and scanning electron microscopy. A 3D-characterization method based on morphological filtering, which allows a holistic view of the surface properties, was applied, and a new qualification scheme for surface microstructures was introduced. Empirical models were also developed and validated for establishing relationships between process parameters and performance criteria. Multi-objective optimization was performed to achieve a minimal value of structure height errors and waviness.
Primer-Treated Ceramic Bracket Increases Shear Bond Strength on Dental Zirconia Surface
Authors: Ju Ga-Youn , Lim Bum-Soon , Moon Wonjoon , Park Shin-Young , Oh Soram , Chung Shin Hye
Published: September 2020

The purpose of this study is to evaluate the shear bond strength (SBS) of a primer-treated ceramic bracket on dental zirconia and to compare it with conventional ceramic bracket bonding on surface-treated zirconia. Sintered and finished dental zirconia was sandblasted. Samples were divided according to the treated surfaces: no treatment (X), ceramic primer on zirconia (Z), ceramic primer on bracket base (B), and ceramic primer on both zirconia and bracket base (ZB). The ceramic bracket was bonded on zirconia and SBS was measured before (T0) and after 10,000 cycles of thermocycling (Tf). The failed surfaces were examined under field emission scanning electron microscope (FE-SEM), and adhesive remnant index (ARI) was evaluated. SBS was significantly higher in ZB and significantly lower in X in both T0 and Tf. There was no significant difference between Z and B. In X and B, adhesive failure occurred while ZB showed mixed failures. There was no apparent change in the zirconia surface except for the existence of some adhesive and resin remnants. The application of ceramic primer on the bracket base increased the bonding strength to the level of conventional bonding with fewer adhesive remnants. The highest bonding strength was obtained when the primer-treated bracket was bonded on the primer-treated zirconia.
Formulation of a Model Resin System for Benchmarking Processing-Property Relationships in High-Performance Photo 3D Printing Applications
Authors: Tu Jianwei , Makarian Kamran , Alvarez Nicolas J. , Palmese Giuseppe R.
Published: September 2020

A well-defined resin system is needed to serve as a benchmark for 3D printing of high-performance composites. This work describes the design and characterization of such a system that takes into account processability and performance considerations. The Grunberg–Nissan model for resin viscosity and the Fox equation for polymer Tg were used to determine proper monomer ratios. The target viscosity of the resin was below 500 cP, and the target final Tg of the cured polymer was 150 °C based on tan-δ peak from dynamic mechanical analysis. A tri-component model resin system, termed DA-2 resin, was determined and fully characterized. The printed polymer exhibited good thermal properties and high mechanical strength after post-cure, but has a comparatively low fracture toughness. The model resin will be used in additive manufacturing of fiber reinforced composite materials as well as for understanding the fundamental processing–property relationships in light-based 3D printing.
Expanding NIST Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles
Authors: DeRose Paul , Tian Linhua , Elsheikh Elzafir , Urbas Aaron , Zhang Yu-Zhong , Wang Lili
Published: September 2020

The National Institute of Standards and Technology (NIST), the National Institutes of Health (NIH) and other industry stakeholders have been working together to enable fluorescence intensities of flow cytometer calibration beads to be assigned quantitative equivalent reference fluorophore (ERF) values with high accuracy and precision. The ultimate goal of this effort is to accurately quantify the number of antibodies bound to individual living cells. The expansion of this effort to assign ERF values to more than 50 fluorescence channels and particles with diameters ranging from 10 μm down to 80 nm is reported here.
Graph-Based Problem Explorer: A Software Tool to Support Algorithm Design Learning While Solving the Salesperson Problem
Authors: Hernández-Sabaté Aura , Albarracín Lluís , Sánchez F. Javier
Published: September 2020

In this article, we present a sequence of activities in the form of a project in order to promote learning on design and analysis of algorithms. The project is based on the resolution of a real problem, the salesperson problem, and it is theoretically grounded on the fundamentals of mathematical modelling. In order to support the students’ work, a multimedia tool, called Graph-based Problem Explorer (GbPExplorer), has been designed and refined to promote the development of computer literacy in engineering and science university students. This tool incorporates several modules to allow coding different algorithmic techniques solving the salesman problem. Based on an educational design research along five years, we observe that working with GbPExplorer during the project provides students with the possibility of representing the situation to be studied in the form of graphs and analyze them from a computational point of view.
Fabrication and Characterization of Curved Compound Eyes Based on Multifocal Microlenses
Authors: Lian Gaoge , Liu Yongshun , Tao KeKai , Xing Huaming , Huang Ruxia , Chi Mingbo , Zhou Wenchao , Wu Yihui
Published: September 2020

Curved compound eyes have generated great interest owing to the wide field of view but the application of devices is hindered for the lack of proper detectors. One-lens curved compound eyes with multi-focal microlenses provide a solution for wide field imaging integrated in a commercial photo-detector. However, it is still a challenge for manufacturing this kind of compound eye. In this paper, a rapid and accurate method is proposed by a combination of photolithography, hot embossing, soft photolithography, and gas-assisted deformation techniques. Microlens arrays with different focal lengths were firstly obtained on a polymer, and then the planar structure was converted to the curved surface. A total of 581 compound eyes with diameters ranging from 152.8 µm to 240.9 µm were successfully obtained on one curved surface within a few hours, and the field of view of the compound eyes exceeded 108°. To verify the characteristics of the fabricated compound eyes, morphology deviation was measured by a probe profile and a scanning electron microscope. The optical performance and imaging capability were also tested and analyzed. As a result, the ommatidia made up of microlenses showed not only high accuracy in morphology, but also imaging uniformity on a focal plane. This flexible massive fabrication of compound eyes indicates great potential for miniaturized imaging systems.
Microphysiological Systems for Neurodegenerative Diseases in Central Nervous System
Authors: Bae Mihyeon , Yi Hee-Gyeong , Jang Jinah , Cho Dong-Woo
Published: September 2020

Neurodegenerative diseases are among the most severe problems in aging societies. Various conventional experimental models, including 2D and animal models, have been used to investigate the pathogenesis of (and therapeutic mechanisms for) neurodegenerative diseases. However, the physiological gap between humans and the current models remains a hurdle to determining the complexity of an irreversible dysfunction in a neurodegenerative disease. Therefore, preclinical research requires advanced experimental models, i.e., those more physiologically relevant to the native nervous system, to bridge the gap between preclinical stages and patients. The neural microphysiological system (neural MPS) has emerged as an approach to summarizing the anatomical, biochemical, and pathological physiology of the nervous system for investigation of neurodegenerative diseases. This review introduces the components (such as cells and materials) and fabrication methods for designing a neural MPS. Moreover, the review discusses future perspectives for improving the physiological relevance to native neural systems.
Biogenic Fe(II-III) Hydroxycarbonate Green Rust Enhances Nitrate Removal and Decreases Ammonium Selectivity during Heterotrophic Denitrification
Authors: Ona-Nguema Georges , Guerbois Delphine , Pallud Céline , Brest Jessica , Abdelmoula Mustapha , Morin Guillaume
Published: September 2020

Nitrification-denitrification is the most widely used nitrogen removal process in wastewater treatment. However, this process can lead to undesirable nitrite accumulation and subsequent ammonium production. Biogenic Fe(II-III) hydroxycarbonate green rust has recently emerged as a candidate to reduce nitrite without ammonium production under abiotic conditions. The present study investigated whether biogenic iron(II-III) hydroxycarbonate green rust could also reduce nitrite to gaseous nitrogen during bacterial nitrate reduction. Our results showed that biogenic iron(II-III) hydroxycarbonate green rust could efficiently decrease the selectivity of the reaction towards ammonium during heterotrophic nitrate reduction by native wastewater-denitrifying bacteria and by three different species of Shewanella: S. putrefaciens ATCC 12099, S. putrefaciens ATCC 8071 and S. oneidensis MR-1. Indeed, in the absence of biogenic hydroxycarbonate green rust, bacterial reduction of nitrate converted 11–42% of the initial nitrate into ammonium, but this value dropped to 1–28% in the presence of biogenic hydroxycarbonate green rust. Additionally, nitrite accumulation did not exceed the 2–13% in the presence of biogenic hydroxycarbonate green rust, versus 0–28% in its absence. Based on those results that enhance the extent of denitrification of about 60%, the study proposes a water treatment process that couples the bacterial nitrite production with the abiotic nitrite reduction by biogenic green rust.
The Use of Tailings to Make Glass as an Alternative for Sustainable Environmental Remediation:
The Case of Osor, Catalonia, Spain
Authors: Alfonso Pura , Tomasa Oriol , Domenech Luis Miguel , Garcia-Valles Maite , Martinez Salvador , Roca Núria
Published: September 2020

Tailings from the Osor fluorite mines release large amounts of potentially toxic elements into the environment. This work is a proposal to remove these waste materials and use them as a raw material in the manufacture of glass. The chemical composition of the tailings was determined by X-ray fluorescence and the mineralogy by X-ray diffraction. Waste materials have SiO2, Al2O3 and CaO contents suitable for a glass production, but Na as NaCO3 has to be added. Two glass formulations, with 80–90% of the residue and 10–20% Na2CO3, have been produced. The crystallization temperatures, obtained by differential thermal analysis, were 875 and 901 °C, and the melting temperatures were 1220 and 1215 °C for the G80-20 and G90-10 glasses, respectively. The transition temperatures of glass were 637 and 628 °C. The crystalline phases formed in the thermal treatment to produce devitrification were nepheline, plagioclase and diopside in the G80-20 glass, and plagioclase and akermanite-gehlenite in the G90-10 glass. The temperatures for the fixed viscosity points, the working temperatures and the coefficient of expansion were obtained. The chemical stability of the glass was tested and results indicate that the potentially toxic elements of the tailings were incorporated into the glass structure.
Graphdiyne Saturable Absorber for Passively Q-Switched Ho3+-Doped Laser
Authors: Zhang Cheng , Hao Qianqian , Zu Yuqian , Zong Mengyu , Guo Jia , Zhang Feng , Ge Yanqi , Liu Jie
Published: September 2020

High-quality all-carbon nanostructure graphdiyne (GDY) saturable absorber was successfully fabricated and saturable absorption properties in the 2 μm region were characterized using a commercial mode-locked laser as a pulsed source. The fabricated GDY was first used as an optical switcher in a passively Q-switched Ho laser. Under absorbed pump power of 2.4 W, the maximum average output power and shortest pulse width were 443 mW and 1.38 µs, at a repetition rate of 29.72 kHz. The results suggest that GDY nanomaterial is a promising candidate as an optical modulator for generation of short pulses in Ho-doped lasers at 2.1 μm.
SERS-Active Pattern in Silver-Ion-Exchanged Glass Drawn by Infrared Nanosecond Laser
Authors: Babich Ekaterina , Kaasik Vladimir , Redkov Alexey , Maurer Thomas , Lipovskii Andrey
Published: September 2020

The irradiation of silver-to-sodium ion-exchanged glass with 1.06-μm nanosecond laser pulses of mJ-range energy results in the formation of silver nanoparticles under the glass surface. Following chemical removal of ~25-nm glass layer reveals a pattern of nanoparticles capable of surface enhancement of Raman scattering (SERS). The pattern formed when laser pulses are more than half-overlapped provides up to ~105 enhancement and uniform SERS signal distribution, while the decrease of the pulse overlap results in an order of magnitude higher but less uniform enhancement.
Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome
Authors: Ali Md. Hossen , Sobze Jean-Marie , Pham Thu Huong , Nadeem Muhammad , Liu Chen , Galagedara Lakshman , Cheema Mumtaz , Thomas Raymond
Published: September 2020

Application of the nanopriming technique to alleviate seed dormancy has shown promising results in various agricultural crop species. However, there is a dearth of knowledge regarding its potential use in native peatland boreal forest species to alleviate seed dormancy and improve their propagation or vigor for forest reclamation activities. Herein, we demonstrate the use of nanopriming with carbon nanotubes (CNT) to alleviate seed dormancy, improved seed germination, and seedling vigor in two boreal peatland species. Bog birch (Betula pumila L.) and Labrador tea (Rhododendron groenlandicum L.) seeds with embryo or seed coat dormancy were nanoprimed with either 20 or 40 µg/mL CNT, cold stratified at 2–4 °C for 15 days, and allowed to germinate at room temperature. The emerged seedlings’ lipidome was assessed to decipher the role of lipid metabolism in alleviating seed dormancy. We observed significant (p < 0.05) improvement in seedling germination and seedling vigor in seeds primed with multiwalled carbon nanotubes functionalized with carboxylic acids. Phosphatidylcholine (PC 18:1/18:3), phosphatidylglycerol (PG 16:1/18:3), and lysophosphatidylcholine (LPC 18:3) molecular species (C18:3 enriched) were observed to be highly correlated with the increased seed germination percentages and the enhanced seedling vigor. Mechanistically, it appears that carbon nanoprimed seeds following stratification are effective in mediating seed dormancy by remodeling the seed membrane lipids (C18:3 enriched PC, PG, and LPC) in both peatland boreal forest species. The study results demonstrate that nanopriming may provide a solution to resolve seed dormancy issues by enhancing seed germination, propagation, and seedling vigor in non-resource boreal forest species ideally suited for forest reclamation following anthropogenic disturbances.
Hybrid Spider Silk with Inorganic Nanomaterials
Authors: Kiseleva Aleksandra P. , Kiselev Grigorii O. , Nikolaeva Valeria O. , Seisenbaeva Gulaim , Kessler Vadim , Krivoshapkin Pavel V. , Krivoshapkina Elena F.
Published: September 2020

High-performance functional biomaterials are becoming increasingly requested. Numerous natural and artificial polymers have already demonstrated their ability to serve as a basis for bio-composites. Spider silk offers a unique combination of desirable aspects such as biocompatibility, extraordinary mechanical properties, and tunable biodegradability, which are superior to those of most natural and engineered materials. Modifying spider silk with various inorganic nanomaterials with specific properties has led to the development of the hybrid materials with improved functionality. The purpose of using these inorganic nanomaterials is primarily due to their chemical nature, enhanced by large surface areas and quantum size phenomena. Functional properties of nanoparticles can be implemented to macro-scale components to produce silk-based hybrid materials, while spider silk fibers can serve as a matrix to combine the benefits of the functional components. Therefore, it is not surprising that hybrid materials based on spider silk and inorganic nanomaterials are considered extremely promising for potentially attractive applications in various fields, from optics and photonics to tissue regeneration. This review summarizes and discusses evidence of the use of various kinds of inorganic compounds in spider silk modification intended for a multitude of applications. It also provides an insight into approaches for obtaining hybrid silk-based materials via 3D printing.
Building an Online Learning Module for Satellite Remote Sensing Applications in Hydrologic Science
Authors: Maggioni Viviana , Girotto Manuela , Habib Emad , Gallagher Melissa A.
Published: September 2020

This article presents an online teaching tool that introduces students to basic concepts of remote sensing and its applications in hydrology. The learning module is intended for junior/senior undergraduate students or junior graduate students with no (or little) prior experience in remote sensing, but with some basic background of environmental science, hydrology, statistics, and programming. This e-learning environment offers background content on the fundamentals of remote sensing, but also integrates a set of existing online tools for visualization and analysis of satellite observations. Specifically, students are introduced to a variety of satellite products and techniques that can be used to monitor and analyze changes in the hydrological cycle. At completion of the module, students are able to visualize remote sensing data (both in terms of time series and spatial maps), detect temporal trends, interpret satellite images, and assess errors and uncertainties in a remote sensing product. Students are given the opportunity to check their understanding as they progress through the module and also tackle complex real-life problems using remote sensing observations that professionals and scientists commonly use in practice. The learning tool is implemented in HydroLearn, an open-source, online platform for instructors to find and share learning modules and collaborate on developing teaching resources in hydrology and water resources.
How Circular Are the European Economies? A Taxonomic Analysis Based on the INEC (Index of National Economies’ Circularity)
Authors: Kasztelan Armand
Published: September 2020

In this paper, the aggregate index of national economies’ circularity (INEC) was proposed and empirically verified. For this purpose, the taxonomic linear ordering method was used, which is a multi-criteria decision-making procedure. This method replaces the analysis of the phenomenon described by a set of indicators with an analysis using one aggregate indicator: the so-called ‘synthetic metric’. Based on 14 circular economy indicators that are available in the Eurostat database, the circularity indexes were constructed for 24 EU countries (including the United Kingdom). This allowed the author, on the one hand, to create a ranking of the countries, and on the other, to assign them to four groups, which were characterized by a similar level of circularity. This paper attempts to answer the following questions: how circular are the European economies? What are the main challenges in achieving circularity in Europe? Taking into account the INEC range [0,1], it should be noted that the level of circularity in the analysed European countries is low (an average of 0.3021). Therefore, the paper indicates the areas requiring improvement in this respect.
An Exploratory Study of a New Psychological Instrument for Evaluating Sustainability: The Sustainable Development Goals Psychological Inventory
Authors: Di Fabio Annamaria , Rosen Marc A.
Published: September 2020

The psychometric properties of the Sustainable Development Goals Psychological Inventory (SDGPI) are verified, by employing quantitative and qualitative approaches, in order to obtain a thorough comprehension of the self-perceptions of the sustainable development goals by the Italian participants in this research. The reliability, dimensionality, and aspects of concurrent validity were tested for the SDGPI. Three unidimensional factors (interest, motivation, and self-efficacy) were shown to be prevalent using exploratory factor analysis. Furthermore, the SDGPI showed adequate concurrent validity and internal consistency in the present study. To broaden the quantitative findings, responses to open-ended questions were analyzed. The results suggest perspectives for future exploration and actions regarding the psychology of sustainable development and sustainability.
The Role of Education in Increasing Awareness and Reducing Impact of Natural Hazards
Authors: Cerulli David , Scott Michael , Aunap Raivo , Kull Ain , Pärn Jaan , Holbrook Jack , Mander Ülo
Published: September 2020

Education could play a role in decreasing and mitigating damages caused by natural disaster. By analysing relationships between level of education and components of the World Risk Index, this study demonstrated an education’s role in natural hazard awareness and mitigation. For this purpose, we analysed relationships between the components of WRI, created an education factor independent of WRI (based on PISA 2018 Science test results), analysed the frequency, magnitude and exposure of natural hazards of an extreme event character in selected countries and analysed the relationships between the education factor and WRI components among the countries. A detailed analysis was performed for 15 countries representing the full global range of natural hazards (frequency, magnitude and exposure to droughts, earthquakes, hurricanes, floods (not related to hurricanes), mass movements, volcanic eruptions, and tsunamis) and level of education. We found that the education factor (ranked and normalised to the maximal value among the considered countries) has significant negative correlation with the following WRI parameters: the Natural Hazard Factor (relative vulnerability, based on the difference between the relative and calculated WRI, ranked and normalised to the maximal value of WRI differences), susceptibility, lack of coping capacities and lack of adaptive capacities (all ranked and normalised to the maximal value). Results indicated that countries at low risk tend to be over-aware while countries at high risk are under-aware of natural hazards. Education can significantly increase awareness of natural hazards and reduce their impact.
Creating Shared Value and Fan Loyalty in the Korean Professional Volleyball Team
Authors: Kim Sang-soo , Baek Woo-yeul , Byon Kevin K. , Ju Sung-bum
Published: September 2020

Creating shared value has been a new strategic management paradigm for professional sport teams around the world. However, despite the active participation of professional sport teams in creating a shared value program, research that addresses its effectiveness appears to be very limited. The present study investigates the influence of sport fans’ perceived creating shared value on team trust and fan loyalty and the moderating effects of sport fans’ altruism on the relationship between creating shared value and team trust in the Korean professional volleyball league. A total of 198 Korean volleyball fans participated in the present study. Results revealed that sport fans’ perceived economic and social values had significant impacts on team trust and, in turn, team trust significantly affected fan loyalty. However, the moderating effect of sport fans’ altruism was not found on the relationships between creating shared value and team trust. Consequently, the present study’s findings may provide professional sport teams’ marketers with the rationale as to the effectiveness of launching creating shared value programs.
Valorization of Orange Peel Waste Using Precomposting and Vermicomposting Processes
Authors: De Medina-Salas Lorena , Giraldi-Díaz Mario Rafael , Castillo-González Eduardo , Morales-Mendoza Laura Elena
Published: September 2020

The industrialization process of oranges generates waste, which is inadequately disposed of; this produces adverse effects on the environment. Among the alternatives for valorization is the vermicomposting process, which consists of the degradation of organic waste through the action of earthworms and microorganisms. Therefore, this research aimed to study this process using orange peel (OP) waste at the laboratory level. For this purpose, it was necessary to determine the degradation conditions through the monitoring of physicochemical parameters (temperature, pH, humidity, organic matter (OM), total organic carbon (TOC), total nitrogen (TN) and the carbon/nitrogen (C/N) ratio). To balance the substrate’s nutrients, load material (LM) that included vegetable waste and eggshells was added to three different mixtures: M1 (50% OP + 50% LM), M2 (40% OP + 60% LM) and M3 (60% OP + 40% LM). To condition the substrate for earthworm (Eisenia fetida) activity, a previous precomposting process was performed. The results showed that all the mixtures fulfilled the requirements for a quality and mature vermicompost; however, the highest concentrations for TN were in the mixtures M1 and M2. The total time required for degradation of the OP waste was 13 weeks.
A Power Flow Control Strategy for Hybrid Control Architecture of DC Microgrid under Unreliable Grid Connection Considering Electricity Price Constraint
Authors: Padhilah Faris Adnan , Kim Kyeong-Hwa
Published: September 2020

This paper presents a power flow control strategy for a hybrid control architecture of the DC microgrid (DCMG) system under an unreliable grid connection considering the constraint of electricity price. To overcome the limitation of the existing schemes, a hybrid control architecture which combines the centralized control and distributed control is applied to control DCMG. By using the hybrid control approach, a more optimal and reliable DCMG system can be constructed even though a fault occurs in the grid or a central controller (CC). The power flow control strategy for the hybrid DCMG control architecture also takes the constraint of electricity price into account for the purpose of minimizing the electricity cost. In the proposed hybrid control, the high bandwidth communication (HBC) link is used in the centralized control to connect the CC with DCMG power agents. On the other hand, the low bandwidth communication (LBC) link is employed to constitute the distributed control. A small size of data is used to exchange the information fast between the agents and CC, or between each agent and its neighbors, which increases the reliability and robustness of the DCMG system in case of a fault in the communication link of the centralized control. A DCMG system with 400-V rated DC-link voltage which consists of a wind power agent, a battery agent, a grid agent, a load agent, and a CC is constructed in this study by using three power converters based on 32-bit floating point digital signal processor (DSP) TMS320F28335 controller. Various simulation and experimental results prove that the proposed scheme improves the system stability and robustness even in the presence of a fault in the communication link of the centralized control. In addition, the proposed scheme is capable of maintaining the DC-link voltage stably at the nominal value without severe transients both in the centralized control and distributed control, as well as both in the grid-connected case and islanded case. Finally, the scalability of the DCMG system is tested by adding and removing additional wind power agent and battery agent during a certain period.