bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated robotics platform with haptic control differentiates subjects with Parkinson’s disease from controls and quantifies the motor effects of levodopa
Authors: Gaprielian Pauline , Scott Stephen H. , Lowrey Catherine , Reid Stuart , Pari Giovanna , Levy Ron
Published: October 2019

Background

The use of integrated robotic technology to quantify the spectrum of motor symptoms of Parkinson’s Disease (PD) has the potential to facilitate objective assessment that is independent of clinical ratings. The purpose of this study is to use the KINARM exoskeleton robot to (1) differentiate subjects with PD from controls and (2) quantify the motor effects of dopamine replacement therapies (DRTs).

Methods

Twenty-six subjects (Hoehn and Yahr mean 2.2; disease duration 0.5 to 15 years) were evaluated OFF (after > 12 h of their last dose) and ON their DRTs with the Unified Parkinson’s Disease Rating Scale (UPDRS) and the KINARM exoskeleton robot. Bilateral upper extremity bradykinesia, rigidity, and postural stability were quantified using a repetitive movement task to hit moving targets, a passive stretch task, and a torque unloading task, respectively. Performance was compared against healthy age-matched controls.

Results

Mean hand speed was 41% slower and 25% fewer targets were hit in subjects with PD OFF medication than in controls. Receiver operating characteristic (ROC) area for hand speed was 0.94. The torque required to stop elbow movement during the passive stretch task was 34% lower in PD subjects versus controls and resulted in an ROC area of 0.91. The torque unloading task showed a maximum displacement that was 29% shorter than controls and had an ROC area of 0.71. Laterality indices for speed and end total torque were correlated to the most affected side. Hand speed laterality index had an ROC area of 0.80 against healthy controls. DRT administration resulted in a significant reduction in a cumulative score of parameter Z-scores (a measure of global performance compared to healthy controls) in subjects with clinically effective levodopa doses. The cumulative score was also correlated to UPDRS scores for the effect of DRT.

Conclusions

Robotic assessment is able to objectively quantify parkinsonian symptoms of bradykinesia, rigidity and postural stability similar to the UPDRS. This integrated testing platform has the potential to aid clinicians in the management of PD and help assess the effects of novel therapies.
Prevalence of breastfeeding in a baby-friendly pediatric practice: an experience in Trieste, Italy
Authors: Milinco Mariarosa , Cattaneo Adriano , Macaluso Anna , Materassi Paola , Di Toro Nicola , Ronfani Luca
Published: October 2019

Background

In a pediatric practice in Italy, actions were undertaken to apply the recommendations for a breastfeeding-friendly physician’s office and to promote the adoption of a semi-reclined or laid-back maternal position in breastfeeding. The aim of this study is to evaluate the effect of the actions implemented, in terms of prevalence of exclusive breastfeeding.

Methods

A historical cohort study was carried out using administrative data routinely collected. All women who gave birth in 2016 and registered their newborns with the pediatric practice were included, only mothers of preterm newborns < 30 weeks gestational age were excluded. The main actions undertaken were: employment of a breastfeeding peer supporter; ensuring unlimited daily access in case of breastfeeding difficulties; provision of individual support to breastfeeding mothers in a dedicated room and advice on the laid-back position; scheduling of weekly meetings of small groups for breastfeeding support. Each infant was followed up for five months. The main study outcomes were duration of exclusive breastfeeding (only breast milk and no other liquids or solids, except for drops of syrups with nutritional supplements or medicines) and prevalence at five months.

Results

A total of 265 newborn infants with a gestational age greater than 30 weeks were registered with the pediatric practice during the study period, about 18% of all infants born in Trieste in that period. Complete data were available for 252 of these (95.1%). The rate of exclusive breastfeeding at five months of age was higher than the one reported for the whole infant population of Trieste and of the Friuli Venezia Giulia Region (62.3% vs. 42.9% vs. 30.3%) in the same period.

Conclusions

The implementation of breastfeeding-friendly pediatric practice and the application of laid-back breastfeeding may improve the rate and duration of exclusive breastfeeding.
The dental anomaly: how and why dental caries and periodontitis are phenomenologically atypical
Authors: Rakhra Dylan
Published: October 2019

Background

Despite their shared origins, medicine and dentistry are not always two sides of the same coin. There is a long history in medical philosophy of defining disease and various medical models have come into existence. Hitherto, little philosophical and phenomenological work has been done considering dental caries and periodontitis as examples of disease and illness.

Methods

A philosophical methodology is employed to explore how we might define dental caries and periodontitis using classical medical models of disease – the naturalistic and normativist. We identify shared threads and highlight how the features of these highly prevalent dental diseases prevent them fitting in either definition. The article describes phenomenology and the current thought around the phenomenology of illness, exploring how and why these dental illnesses might integrate into a phenomenological model.

Results

We discover that there are some features particular to dental caries and periodontitis: ubiquity, preventability and hyper-monitorablility. Understanding the differences that these dental diseases have compared to many other classically studied diseases leads us to ethical questions concerning how we might manage those who have symptoms and seek treatment. As dental caries and periodontitis are common, preventable and hyper-monitorable, it is suggested that these features affect the phenomenology of these illnesses. For example, if we experience dental illness when we have consciously made decisions that have led to it, do we experience them differently to those rarer illnesses that we cannot expect? Other diseases share these features are discussed.

Conclusions

This paper highlights the central differences between the classical philosophical notion of disease in medicine and the dental examples of caries and periodontitis. It suggests that a philosophical method of conceptualising medical illness - phenomenology - should not be applied to these dental illnesses without thought. A phenomenological analysis of any dental illness is yet to be done and this paper highlights why a separate strand of phenomenology should be explored, instead of employing those that are extant. The article concludes with suggestions for further research into the nascent field of the phenomenology of dental illness and aims to act as a springboard to expose the dental sphere to this philosophical method of analysis.
Feasibility and preliminary clinical results of linac-based Stereotactic Body Radiotherapy for spinal metastases using a dedicated contouring and planning system
Authors: Giaj-Levra Niccolò , Niyazi Maximilian , Figlia Vanessa , Napoli Giuseppe , Mazzola Rosario , Nicosia Luca , Corradini Stefanie , Ruggieri Ruggero , Minniti Giuseppe , Alongi Filippo
Published: October 2019

Background

Stereotactic radiosurgery (SRS) and stereotactic body radiotherapy (SBRT) are well established local treatment approaches in several cancer settings. Although SBRT is still under investigation for spinal metastases, promising results in terms of a high effectiveness and optimal tolerability have been recently published on this topic. For spinal SBRT, one of the most relevant issues is represented by the inter-observer variability in target definition. Recently, several technological innovations, including specific tools such as multimodality-imaging (computed tomography (CT), magnetic resonance imaging (MRI) and positron emission tomography (PET-CT), automated volumes contouring and planning, could allow clinicians to minimize the uncertainties related to spinal SBRT workflow. Aim of this study is to report the feasibility of the clinical application of a dedicated software (Element®, Brainlab™ Germany) for spinal metastases SBRT.

Material and method

The patient selection criteria for SBRT in spinal metastases were the following: age > 18 years, diagnosis of spinal metastases (n ≤ 3), life expectancy > 3 months, controlled primary tumor or synchronous diagnosis and Spinal Instability Neoplastic Score (SINS) ≤ 12 points. All radiation target volumes were defined and planned with the support of the dedicated software Elements® (Brainlab™ Germany). Different dose prescription have been used: 12 Gy in single fraction, 12 Gy, 18 Gy, 21 Gy and 24 Gy in 3 fractions. Toxicity was assessed according to the Common Terminology Criteria for Adverse Events (CTCAE) v4.0. SPSS version 20 was used for statistical analysis.

Results

From April 2018 to April 2019, 54 spinal metastases in 32 recruited patients were treated with Linac-based SBRT. With a median follow-up of 6 months (range 3–12), local control rates at 6 months and 9 months were 86 and 86%, respectively. No adverse events ≥3 grade were observed.

Conclusions

This preliminary experience shows that with respect to acute toxicity and early clinical response, linac-based using Elements® Spine SRS is a feasible and effective approach.
Metabolomics profiling of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata extracts using UPLC-Q/TOF-MS
Authors: Zhang Zhaoyan , Yang Liang , Huang Xiaoyan , Gao Yue
Published: October 2019

Background

The side effects caused by Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Praeparata (PMRP) have often appeared globally. There is no research on the changes of endogenous metabolites among PMR- and PMRP-treated rats. The aim of this study was to evaluate the varying metabolomic effects between PMR- and PMRP-treated rats. We tried to discover relevant differences in biomarkers and endogenous metabolic pathways.

Methods

Hematoxylin and eosin staining and immunohistochemistry staining were performed to find pathological changes. Biochemical indicators were also measured, one-way analysis of variance with Dunnett’s multiple comparison test was used for biochemical indicators comparison among various groups. Metabolomics analysis based on ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-Q/TOF-MS) was performed to find the changes in metabolic biomarkers. Multivariate statistical approaches such as principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA) were applied to reveal group clustering trend, evaluate and maximize the discrimination between the two groups. MetaboAnalyst 4.0 was performed to find and confirm the pathways.

Results

PMR extracts exhibited slight hepatotoxic effects on the liver by increasing aspartate and alanine aminotransferase levels. Twenty-nine metabolites were identified as biomarkers, belonging to five pathways, including alpha-linolenic acid metabolism, taurine and hypotaurine metabolism, glycerophospholipid metabolism, arginine and proline metabolism, and primary bile acid biosynthesis.

Conclusion

This study provided a comprehensive description of metabolomic changes between PMR- and PMRP-treated rats. The underlying mechanisms require further research.
A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering
Authors: Mohammadzadeh Leila , Rahbarghazi Reza , Salehi Roya , Mahkam Mehrdad
Published: October 2019

Background

The main issue in cutaneous regeneration is to develop engineered scaffolds based on natural extracellular matrix to promote dynamics of skin progenitor cells and accelerate differentiation into mature keratinocytes.

Methods

In this study, nanofibrous scaffolds composed of a blend poly (ɛ-caprolactone) (PCL), silk fibroin (SF), soluble eggshell membrane (SESM), andAloe vera (AV) gel were developed by electrospinning method and human basal cells were used to examine differentiation capacity toward keratinocyte-like cells. For this propose, cells were allocated to four distinct groups; control, PCL/SF, PCL/SF/SESM, and PCL/SF/SESM/AV. In all groups, cells were incubated with differentiation medium. Morphology, composition, hydrophilicity and mechanical features of PCL/SF, PCL/SF/SESM and PCL/SF/SESM/AV nanofibers were studied by scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), water contact angle and tensile tests. To examine the orientation of basal cells to mature keratinocytes, we performed immunofluorescence analysis by monitoring cytokeratin-19. The expression of genes such as involucrin, keratin-14 and -5 was monitored by real-time PCR assay.

Results

PCL/SF, PCL/SF/SESM, and PCL/SF/SESM/AV had suitable physic chemical indices and biological activities to be applied as biomimetic scaffolds for the restoration cutaneous tissue. Compared to control, we found an increased basal cell proliferation at 7 and 14 days after plating on scaffolds and reach maximum levels in group PCL/SF/SESM/AV on day 14 (p < 0.05). Electron microscopy showed cell flattening, morphological adaptation. An integrated cell-to-cell connection was generated after cell seeding on scaffolds in all groups. Immunofluorescence imaging showed the ability of basal cells to synthesize cytokeratin-19 in PCL/SF, PCL/SF/SESM, and positive control cells after exposure to differentiation medium. However, these values were less in PCL/SF/SESM/AV compared to other groups. Real-time PCR analysis showed the potency of all scaffolds to induce the transcription of involucrin, keratin-14 and -5, especially involucrin in PCL/SF/SESM/AV group compared to the negative control.

Conclusion

Modulation of scaffolds with natural biopolymers could enable us to synthesize structures appropriate for cutaneous regeneration.
Predicting metabolite-disease associations based on KATZ model
Authors: Lei Xiujuan , Zhang Cheng
Published: October 2019

Background

Increasing numbers of evidences have illuminated that metabolites can respond to pathological changes. However, identifying the diseases-related metabolites is a magnificent challenge in the field of biology and medicine. Traditional medical equipment not only has the limitation of its accuracy but also is expensive and time-consuming. Therefore, it’s necessary to take advantage of computational methods for predicting potential associations between metabolites and diseases.

Results

In this study, we develop a computational method based on KATZ algorithm to predict metabolite-disease associations (KATZMDA). Firstly, we extract data about metabolite-disease pairs from the latest version of HMDB database for the materials of prediction. Then we take advantage of disease semantic similarity and the improved disease Gaussian Interaction Profile (GIP) kernel similarity to obtain more reliable disease similarity and enhance the predictive performance of our proposed computational method. Simultaneously, KATZ algorithm is applied in the domains of metabolomics for the first time.

Conclusions

According to three kinds of cross validations and case studies of three common diseases, KATZMDA is worth serving as an impactful measuring tool for predicting the potential associations between metabolites and diseases.
Emerging agents and regimens for hepatocellular carcinoma
Authors: Zhu Xiao-Dong , Sun Hui-Chuan
Published: October 2019

Liver cancer, mostly hepatocellular carcinoma (HCC), is the second leading cause of cancer mortality globally. Most patients need at least one systemic therapy at different phases of their treatment for HCC. Sorafenib was the first agent shown to improve the survival of patients with advanced HCC. A decade after the approval of sorafenib, most agents failed to improve patient survival more than sorafenib. In recent years, treatment practices have changed, with lenvatinib as another first-line treatment choice and regorafenib, ramucirumab, and cabozantinib as second-line treatment options. Anti-PD-1 antibodies, including nivolumab, pembrolizumab, and camrelizumab, have demonstrated promising anti-tumor effects as monotherapy for advanced HCC in phase II clinical trials. The combination of an anti-PD-1 antibody and an anti-angiogenesis agent has shown more potent anti-tumor effects in early phase clinical trials and is now the hotspot in clinical studies. Furthermore, these agents are investigated in combination treatment with surgery or other loco-regional therapies in patients with early or intermediate-stage HCC.
Polo-like kinase 3 inhibits glucose metabolism in colorectal cancer by targeting HSP90/STAT3/HK2 signaling
Authors: Ou Baochi , Sun Hongze , Zhao Jingkun , Xu Zhuoqing , Liu Yuan , Feng Hao , Peng Zhihai
Published: October 2019

Background

Polo-like kinase 3 (PLK3) has been documented as a tumor suppressor in several types of malignancies. However, the role of PLK3 in colorectal cancer (CRC) progression and glucose metabolism remains to be known.

Methods

The expression of PLK3 in CRC tissues was determined by immunohistochemistry. Cells proliferation was examined by EdU, CCK-8 and in vivo analyses. Glucose metabolism was assessed by detecting lactate production, glucose uptake, mitochondrial respiration, extracellular acidification rate, oxygen consumption rate and ATP production. Chromatin immunoprecipitation, luciferase reporter assays and co-immunoprecipitation were performed to explore the signaling pathway. Specific targeting by miRNAs was determined by luciferase reporter assays and correlation with target protein expression.

Results

PLK3 was significantly downregulated in CRC tissues and its low expression was correlated with worse prognosis of patients. In vitro and in vivo experiments revealed that PLK3 contributed to growth inhibition of CRC cells. Furthermore, we demonstrated that PLK3 impeded glucose metabolism via targeting Hexokinase 2 (HK2) expression. Mechanically, PLK3 bound to Heat shock protein 90 (HSP90) and facilitated its degradation, which led to a significant decrease of phosphorylated STAT3. The downregulation of p-STAT3 further suppressed the transcriptional activation of HK2. Moreover, our investigations showed that PLK3 was directly targeted by miR-106b at post-transcriptional level in CRC cells.

Conclusion

This study suggests that PLK3 inhibits glucose metabolism by targeting HSP90/STAT3/HK2 signaling and PLK3 may serve as a potential therapeutic target in colorectal cancer.
A randomized controlled trial comparing lithium plus valproic acid versus lithium plus carbamazepine in young patients with type 1 bipolar disorder: the LICAVAL study
Authors: Missio Giovani , Moreno Doris Hupfeld , Demetrio Frederico Navas , Soeiro-de-Souza Marcio Gerhardt , dos Santos Fernandes Fernando , Barros Vivian Boschesi , Moreno Ricardo Alberto
Published: October 2019

Background

Treatment of bipolar disorder (BD) usually requires drug combinations. Combinations of lithium plus valproic acid (Li/VPA) and lithium plus carbamazepine (Li/CBZ) are used in clinical practice but were not previously compared in a head-to-head trial.

Objective

The objective of this trial was to compare the efficacy and tolerability of Li/VPA versus Li/CBZ in treating type 1 BD in any phase of illness in young individuals.

Methods

LICAVAL was a randomized, unicenter, open-label, parallel-group trial that was conducted from January 2009 to December 2012 in a tertiary hospital in São Paulo, Brazil. Participants were between 18 and 35 years old and were followed up for 2 years. Our primary outcome was the number of participants achieving/maintaining response and remission during the acute and maintenance phases of BD treatment, respectively. Other outcomes assessed were symptom severity and adverse events throughout the study. In the analysis of the primary outcome, we compared groups by using a two-way repeated measures analysis of variance and estimated effect sizes by using Cohen’s d.

Results

Of our 64 participants, 36 were allocated to Li/VPA and 28 to Li/CBZ. Our sample was composed predominantly of females (66.6%) and the average age was 27.8 years. A total of 27 (45.0%) participants had depression, 17 (28.3%) had mania/hypomania, and 16 (26.7%) had a mixed state. We found no between-group differences in CGI-BP (Clinical Global Impression Scale modified for use in bipolar disorder) scores (P = 0.326) or in any other outcome. Side effects differed significantly between groups only in the first week of treatment (P = 0.021), and there were more side effects in the Li/VPA group. Also, the Li/VPA group gained weight (+2.1 kg) whereas the Li/CBZ group presented slight weight loss (−0.2 kg).

Conclusion

Our study suggests that Li/VPA and Li/CBZ have similar efficacy and tolerability in BD but that Li/CBZ might have metabolic advantages in the long term.

Trial registration

ClinicalTrials.gov identifier: NCT00976794. Registered on September 9, 2009.
Protein hydrolysis and fermentation under methanogenic and acidifying conditions
Authors: Duong Thu Hang , Grolle Katja , Nga Tran Thi Viet , Zeeman Grietje , Temmink Hardy , van Eekert Miriam
Published: October 2019

Background

Many kinds of wastewaters contain appreciable quantities of protein. Anaerobic processes are suitable for the treatment of wastewater high in organics to achieve pollution control and recovery of energy as methane and hydrogen, or intermediates for production of biofuels and valuable biochemicals. A distinction between protein hydrolysis and amino acid fermentation, especially for dissolved proteins, is needed to target which one is truly rate-limiting and to effectively harvest bioproducts during anaerobic conversion of these wastewaters. This study explored mesophilic anaerobic hydrolysis and amino acid fermentation of gelatine, as a model for dissolved proteins, at pH 7 and at pH 5.

Results

The results showed that at pH 7, protein hydrolysis (first-order rate of 0.15 h−1) was approximately 5 times faster than acidification of the hydrolysis products (first-order rate of 0.03 h−1), implying that not hydrolysis but acidification was the rate-limiting step in anaerobic dissolved protein degradation. This was confirmed by (temporary) accumulation of amino acids. Nineteen different amino acids were detected during the first 8 incubation hours of gelatine at neutral pH and the total chemical oxygen demand (COD) of these 19 amino acids was up to approximately 40% of the COD of the gelatine that was added. Protein hydrolysis at pH 5 was 2–25 times slower than at pH 7. Shifting the initial pH from neutral to acidic conditions (pH 5) inhibited protein degradation and changed the volatile fatty acids (VFA) product profile. Furthermore, the presence or absence of methanogenic activity did not affect the rates of protein hydrolysis and acidification.

Conclusions

The findings in this study can help to set a suitable solid retention time to accomplish anaerobic degradation of protein-rich wastewaters in continuous reactor systems. For example, if the target is harvesting VFAs, methanogens can be washed-out for a shorter retention time while amino acid fermentation, instead of hydrolysis as assumed previously, will govern the design and solutions to improve the system dealing with dissolved proteins.
Simultaneous upgrading of biomass-derived sugars to HMF/furfural via enzymatically isomerized ketose intermediates
Authors: Wang Wei , Mittal Ashutosh , Pilath Heidi , Chen Xiaowen , Tucker Melvin P. , Johnson David K.
Published: October 2019

Background

Recently, exploring fermentative or chemical pathways that convert biomass-derived sugars to fuels/chemicals has attracted a lot of interest from many researchers. We are investigating a hydrocarbon pathway from mixed sugars via 5-hydroxymethyl furfural (HMF) and furfural intermediates. To achieve this goal, we must first convert glucose and xylose to HMF and furfural in favorable yields. Current processes to produce HMF/furfural generally involve the use of acid catalysts in biphasic systems or solvents such as ionic liquids. However, the yield from transforming glucose to HMF is lower than the yield of furfural from xylose.

Results

In this study, we present an efficient chemical pathway simultaneously transforming glucose and xylose to HMF and furfural via ketose intermediates, i.e., fructose and xylulose, which were generated from glucose and xylose via enzymatic isomerization. In the enzymatic isomerization, by adding sodium borate to complex with the ketoses, xylose conversion reached equilibrium after 2 h with a conversion of 91% and glucose conversion reached 84% after 4 h. By enzymatically isomerizing the aldoses to ketoses, the following dehydration reactions to HMF and furfural could be performed at low process temperatures (i.e., 110–120 °C) minimizing the side reactions of the sugars and limiting the degradation of furfurals to humins and carboxylic acids. At 120 °C, pH 0.5, and 15 min reaction time, mixed ketose sugars were converted to HMF and furfural in yields of 77% and 96%, respectively (based on starting aldose concentrations).

Conclusion

Taken together, our results demonstrate that this combined biological and chemical process could be an effective pathway to simultaneously convert biomass-derived glucose and xylose to HMF and furfural, for use as intermediates in the production of hydrocarbons.
Early treatment with ambrisentan of mildly elevated mean pulmonary arterial pressure associated with systemic sclerosis: a randomized, controlled, double-blind, parallel group study (EDITA study)
Authors: Pan Zixuan , Marra Alberto M. , Benjamin Nicola , Eichstaedt Christina A. , Blank Norbert , Bossone Eduardo , Cittadini Antonio , Coghlan Gerry , Denton Christopher P. , Distler Oliver , Egenlauf Benjamin , Fischer Christine , Harutyunova Satenik , Xanthouli Panagiota , Lorenz Hanns-Martin , Grünig Ekkehard
Published: October 2019

Objective

The objective of this randomized, placebo-controlled, double-blind, parallel group, trial was to assess the effect of ambrisentan on mean pulmonary arterial pressure (mPAP) in patients with systemic sclerosis (SSc) and mildly elevated pulmonary hypertension (PH).

Methods

Thirty-eight SSc patients with mildly elevated mPAP at rest between 21 and 24 mmHg and/or > 30 mmHg during low-dose exercise were randomly assigned to treatment with either ambrisentan 5–10 mg/day or placebo. Right heart catheterization and further clinical parameters were assessed at baseline and after 6 months. The primary endpoint was the difference of mPAP change at rest between groups.

Results

After 6 months, the two groups did not differ in the primary endpoint (ambrisentan mPAP − 1 ± 6.4 mmHg vs. placebo − 0.73 ± 3.59 mmHg at rest, p = 0.884). However, three patients from the placebo group but none of the ambrisentan group progressed to SSc-associated pulmonary arterial hypertension. Furthermore, ambrisentan treatment showed significant improvements in the secondary endpoints cardiac index (CI) and pulmonary vascular resistance (PVR) at rest (CI 0.36 ± 0.66 l/min/m2 vs. − 0.31 ± 0.71 l/min/m2, p = 0.010; PVR − 0.70 ± 0.78 WU vs. 0.01 ± 0.71 WU, p = 0.012) and during exercise (CI 0.7 ± 0.81 l/min/m2 vs. − 0.45 ± 1.36 l/min/m2, p = 0.015; PVR − 0.84 ± 0.48 WU vs. − 0.0032 ± 0.34 WU, p < 0.0001).

Conclusion

This is the first randomized, double-blind, placebo-controlled study testing the effect of ambrisentan in patients with mildly elevated mPAP and/or exercise PH. The primary endpoint change in mPAP did only tendentially improve in the ambrisentan group, but the significant improvement of other hemodynamic parameters points to a possible benefit of ambrisentan and will be helpful to design future trials.

Trial registration

www.ClinicalTrials.gov, unique identifier NCT: NCT02290613, registered 14th of November 2014.
Hyperhomocysteinemia is an independent risk factor of atherosclerosis in patients with metabolic syndrome
Authors: Piazzolla Giuseppina , Candigliota Mafalda , Fanelli Margherita , Castrovilli Anna , Berardi Elsa , Antonica Gianfranco , Battaglia Stefano , Solfrizzi Vincenzo , Sabbà Carlo , Tortorella Cosimo
Published: October 2019

Background

Metabolic syndrome (MetS) is a clinical condition potentially promoting the development of atherosclerotic disease. To date, the clinical impact of elevated serum homocysteine (Hcy) levels in MetS is still under discussion. The aim of this cross sectional study was to evaluate the relationship between MetS and hyperhomocysteinemia and the potential role of Hcy in the pathogenesis of atherosclerotic complications of MetS.

Methods

We recruited 300 outpatients with MetS. All patients underwent a medical history collection, physical examination, blood sampling and carotid ultrasound echo-color Doppler. According to Hcy levels, MetS patients were divided into two groups: “normal” (< 10.7 μmol/l; n = 140, group 1) and “high” Hcy (≥ 10.7 μmol/l; n = 160, group 2). Comparisons between groups were made by Student’s t-test or Chi-square test. The effects of potential covariates on group differences were evaluated by general linear models. The relationships between continuous variables were assessed by simple or multiple correlation and by linear regression. Multiple regression models were built to evaluate the effects of Hcy, together with other potential risk factors, on carotid atherosclerosis.

Results

Patients with high Hcy were predominantly male and slightly older than group 1 patients. Smokers and non-smokers exhibited similar Hcy levels, nor was a statistical relationship between pack-years and Hcy observed. Group 2 showed lower levels of folic acid, vitamin D, high density lipoprotein (HDL)-cholesterol and glomerular filtration rate (e-GFR) than group 1, but higher levels of C-peptide, uric acid and triglycerides. In all patients, Hcy was positively correlated with C-peptide and uric acid and negatively with folic acid and e-GFR. Intima-media thickness (IMT) and carotid stenosis degree were significantly higher in patients with high Hcy and a positive relationship between Hcy and both IMT and carotid stenosis was detected in all patients. Finally, Hcy atherogenic effects were independent of other well-known atherosclerosis risk factors.

Conclusions

Our results highlight a link between MetS and hyperhomocysteinemia and a direct effect of Hcy on atherogenic process during MetS. Early correction of folic acid levels may contribute to prevent cardiovascular complications in MetS patients.
Effects of comprehensive sexuality education on the comprehensive knowledge and attitude to condom use among first-year students in Arba Minch University: a quasi-experimental study
Authors: Boti Negussie , Hussen Sultan , Shegaze Mulugeta , Shibru Simon , Shibiru Tamiru , Zerihun Eshetu , Godana Wanzahun , Abebe Sintayehu , Gebretsadik Woyinshet , Desalegn Nathan , Temtime Zebene
Published: October 2019

Objective

To assess the effect of comprehensive sexuality education on the comprehensive knowledge and attitude to condom use among first-year students at Arba Minch University.

Results

A total of 832 students participated at a baseline, and 820 students participated at the posttest. This study found that there was a significant effect on changing students’ knowledge and attitude towards a condom. In the education group, the students’ average change of comprehensive condom knowledge score was 0.229 higher than the average score of students’ in the control group (ATE = 0.229, 95% CI 0.132 to 0.328; p < 0.001). The average change of attitude toward condom score of students’ in the education group was 1.834 higher than the average change score of students’ in the control group (ATE = 1.834, 95% CI 1.195 to 2.772; p < 0.001).This study provides further evidence on the effectiveness of comprehensive sexuality education in terms of knowledge and attitude towards a condom. Therefore, the implementation of this education should be strengthened in order the prevent youths from STI/HIV and unintended pregnancies.
Detection of partial loss of hippocampal striation at 1.5 Tesla magnetic resonance imaging
Authors: Sen Anitha , Sankaran Sudhakaran
Published: October 2019

Objectives

Partial loss of hippocampal striation (PLHS) is recently described in 3 T and 7 T MR imaging as a sensitive indicator of hippocampal sclerosis. Primary objective: We described the demographic characteristics of the population with seizure disorder having PLHS at 1.5 T MR imaging and tried to see the relation of PLHS to the classic signs of hippocampal sclerosis. Secondary objective: PLHS was also looked for in a small control population that had no seizure history.

Methods

This retrospective study had the approval of the institutional review board. In patients demonstrating PLHS on oblique coronal T2-weighted images, the following were recorded: age, sex, EEG findings, side of PLHS, hippocampal atrophy and high signal intensity of the hippocampus. In control population, the following were recorded: age, sex, presence/absence of PLHS and indication for imaging.

Results

The 116 PLHS subjects (age range 2–73 years) included 62 males and 54 females. Sixty-six (56.9%) of our PLHS subjects were less than 18 years of age: 44 (37.9%) under the age of 12 years and 22 (19%) of 12–18 years of age. Classic signs of hippocampal sclerosis were found in only 7 (6%) of the 116 subjects showing PLHS. All patients with classic signs showed PLHS on the same side. Of the control population (25 subjects, age range 3–76 years, 17 males and 8 females), one showed PLHS—he was a treated case of CNS lymphoma with gliotic changes, though there was no history of seizure.

Conclusion

PLHS is demonstrated at 1.5 T in both adult and paediatric population in this article and is much more common than the classic signs of hippocampal sclerosis (increased signal intensity and volume loss).
Ralstonia mannitolilytica sepsis: a case report
Authors: Owusu Michael , Acheampong Godfred , Annan Augustina , Marfo Kwadwo Sarfo , Osei Isaac , Amuasi John , Sarpong Nimako , Im Justin , Mogeni Ondari D. , Chiang Hsin-Ying , Kuo Chih-Horng , Jeon Hyon Jin , Panzner Ursula , Park Se Eun , Marks Florian , Owusu-Dabo Ellis , Adu-Sarkodie Yaw
Published: October 2019

Background

Ralstonia mannitolilyticais an emerging opportunistic pathogen that is associated with severe disease, including septic shock, meningitis, and renal transplant infections. Reports on this pathogen are limited, however, especially on the African continent.

Case presentation

A 2-year-old Akan child was presented to a hospital in the northeastern part of Ghana with a 1-week history of fever and chills. We identifiedRalstonia mannitolilyticain her blood culture using both conventional and 16S ribosomal deoxyribonucleic acid (rDNA) techniques. The patient’s condition improved clinically upon treatment with cefuroxime.

Conclusion

Our report highlights the potential ofRalstonia mannitolilyticato cause sepsis and thus emphasizes the need for improved laboratory diagnosis and evidence for use of appropriate antibiotics in rural settings of Africa, where presumptive treatment using antimicrobial agents is rife.
Existence of solutions for equations and inclusions of multiterm fractional q-integro-differential with nonseparated and initial boundary conditions
Authors: Samei Mohammad Esmael , Khalilzadeh Ranjbar Ghorban , Hedayati Vahid
Published: October 2019

The goal of this paper is to investigate existence of solutions for the multiterm nonlinear fractional q-integro-differential ${}^{c}D_{q}^{\alpha } u(t)$

D
q
α

c

u
(
t
)
in two modes equations and inclusions of order $\alpha\in(n -1, n]$
α
∈
(
n
−
1
,
n
]
, with non-separated boundary and initial boundary conditions where the natural number n is more than or equal to five. We consider a Carathéodory multivalued map and use Leray–Schauder and Covitz–Nadler famous fixed point theorems for finding solutions of the inclusion problems. Besides, we present results whenever the multifunctions are convex and nonconvex. Lastly, we give some examples illustrating the primary effects.
On weighted integrability of functions defined by trigonometric series with p-bounded variation coefficients
Authors: Szal Bogdan , Kubiak Maciej
Published: October 2019

In this paper we introduce new classes of p-bounded variation sequences and give a sufficient and necessary condition for weighted integrability of trigonometric series with coefficients belonging to these classes. This is a generalization of the results obtained by the first author [J. Inequal. Appl. 2010:1–19, 2010] and Dyachenko and Tikhonov [Stud. Math. 193(3):285–306, 2009].
Composite inertial subgradient extragradient methods for variational inequalities and fixed point problems
Authors: Ceng Lu-Chuan , Yuan Qing
Published: October 2019

In this paper, we introduce and investigate composite inertial gradient-based algorithms with a line-search process for solving a variational inequality problem (VIP) with a pseudomonotone and Lipschitz continuous mapping and a common fixed-point problem (CFPP) of finitely many nonexpansive mappings and a strictly pseudocontractive mapping in the framework infinite-dimensional Hilbert spaces. The proposed algorithms are based on an inertial subgradient–extragradient method with the line-search process, hybrid steepest-descent methods, viscosity approximation methods and Mann iteration methods. Under weak conditions, we prove strong convergence of the proposed algorithms to the element in the common solution set of the VIP and CFPP, which solves a certain hierarchical VIP defined on this common solution set.
Uniqueness result for a fractional diffusion coefficient identification problem
Authors: Jday Fadhel , Mdimagh Ridha
Published: October 2019

In this paper, we establish an identifiability result for the coefficient identification problem in a fractional diffusion equation in a bounded domain from the observation of the Cauchy data on particular subsets of the boundary.
Existence of multiple positive solutions for nonhomogeneous fractional Laplace problems with critical growth
Authors: Wang Fang , Zhang Yajing
Published: October 2019

We prove the existence of multiple positive solutions of fractional Laplace problems with critical growth by using the method of monotonic iteration and variational methods.
Exact dynamical behavior for a dual Kaup–Boussinesq system by symmetry reduction and coupled trial equations method
Authors: Li Wen-He , Wang Yong
Published: October 2019

We propose a coupled trial equation method for a coupled differential equations system. Furthermore, according to the invariant property under the translation, we give the symmetry reduction of a dual Kaup–Boussinesq system, and then we use the proposed trial equation method to construct its exact solutions which describe its dynamical behavior. In particular, we get a cosine function solution with a constant propagation velocity, which shows an important periodic behavior of the system.
Stress among university students: factorial structure and measurement invariance of the Italian version of the Effort-Reward Imbalance student questionnaire
Authors: Portoghese Igor , Galletta Maura , Porru Fabio , Burdorf Alex , Sardo Salvatore , D’Aloja Ernesto , Finco Gabriele , Campagna Marcello
Published: October 2019

Background

In the last decade academic stress and its mental health implications amongst university students has become a global topic. The use of valid and theoretically-grounded measures of academic stress in university settings is crucial. The aim of this study was to examine the factorial structure, reliability and measurement invariance of the short student version of the effort-reward imbalance questionnaire (ERI-SQ).

Methods

A total of 6448 Italian university students participated in an online cross-sectional survey. The factorial structure was investigated using exploratory factor analysis and confirmatory factor analysis. Finally, the measurement invariance of the ERI-SQ was investigated.

Results

Results from explorative and confirmatory factor analyses showed acceptable fits for the Italian version of the ERI-SQ. A modified version of 12 items showed the best fit to the data confirming the 3-factor model. Moreover, multigroup analyses showed metric invariance across gender and university course (health vs other courses).

Conclusions

In sum, our results suggest that the ERI-SQ is a valid, reliable and robust instrument for the measurement of stress among Italian university students.
Large dependency of charge distribution in a tropical cyclone inner core upon aerosol number concentration
Authors: Sato Yousuke , Miyamoto Yoshiaki , Tomita Hirofumi
Published: October 2019

The impacts of aerosols on the charge distribution of hydrometeors and lightning flash density in a tropical cyclone (TC) were investigated using a meteorological model coupled with an explicit lightning model. The meteorological model successfully simulated the tripole structure of charge density distribution in a TC, as reported by previous studies. The impacts of aerosols were investigated through a sensitivity experiment with changing the aerosol number concentration. The tripole structure became unclear with increasing aerosol number concentrations. The positive charge distribution located in the lower layer was not seen, and raindrops with negative charge distribution reached the surface. As a result, the vertical structure of the charge density was dipolar in the polluted case. As the tripole structure shifted to dipole, the magnitude of the electric field tended to be large, and the flash number was large. By contrast, in the pristine case, the tripole structure was dominant, and the flash number was much smaller than in the polluted case.
Fungi as source for new bio-based materials: a patent review
Authors: Cerimi Kustrim , Akkaya Kerem Can , Pohl Carsten , Schmidt Bertram , Neubauer Peter
Published: October 2019

Background

The circular economy closes loops in industrial manufacturing processes and minimizes waste. A bio-based economy aims to replace fossil-based resources and processes by sustainable alternatives which exploits renewable biomass for the generation of products used in our daily live. A current trend in fungal biotechnology—the production of fungal-based biomaterials—will contribute to both.

Results

This study gives an overview of various trends and development applications in which fungal mycelium is used as new and sustainable biomaterial. A patent survey covering the last decade (2009–2018) yielded 47 patents and patent applications claiming fungal biomass or fungal composite materials for new applications in the packaging, textile, leather and automotive industries. Furthermore, fungal-based materials are envisaged for thermal insulation and as fire protection materials. Most patents and patent applications describe the use of different lignin- and cellulose-containing waste biomass as substrate for fungal cultivations, covering 27 different fungal species in total. Our search uncovered that most patent activities are on-going in the United States and in China.

Conclusion

Current patent developments in the field suggest that fungal bio-based materials will considerable shape the future of material sciences and material applications. Fungal materials can be considered as an excellent renewable and degradable material alternative with a high innovation potential and have the potential to replace current petroleum-based materials.
Total thymectomy for thymic lymphoepithelioma-like carcinoma—report of two cases
Authors: Kawagishi Sachi , Ose Naoko , Minami Masato , Funaki Soichiro , Kanou Takashi , Kimura Kenji , Taniguchi Seiji , Morii Eiichi , Shintani Yasushi
Published: October 2019

Background

Thymic carcinoma has been classified into 12 subtypes, thymic lymphoepithelioma-like carcinoma (LELC) is a type of them, and has a pathological organization similar to that of lymphoepithelioma, an undifferentiated type of nasopharyngeal carcinoma. According to a report from the International Thymic Malignancy Interest Group (ITMIG), thymic LELC is a rare tumor and accounts for 6% of all thymic carcinoma cases. We report two cases of surgical resection for thymic LELC and perform a search of other reports of thymic LELC, and clinical manifestations and follow-up data thus obtained are summarized.

Case presentation

Two patients underwent surgical resection for thymic LELC. In both, tumors were detected in the anterior mediastinum and a total thymectomy was performed. Each was diagnosed with thymic LELC and classified in accordance with the Masaoka staging system as modified stage II. In recent examinations, one patient was doing well after undergoing total resection, whereas early recurrence of distant lymph node metastasis was noted in the other at 5 months after the total resection procedure and died thereafter from a different disease.

Conclusion

We report two cases of surgical resection for thymic LELC. A successful total resection may positively affect prognosis: thus, long-term follow-up examinations must be performed.
Compulsory coding in education: liberal-humanism, Baudrillard and the ‘problem’ of abstraction
Authors: Tamatea Laurence
Published: October 2019

Internationally, coding is increasingly introduced into primary and junior high schools (children generally aged between 5 and 15) on a compulsory basis, though not all stakeholders support this ‘initiative’. In response to the public reception, discussion highlights popular argument around compulsory coding in school education. This is an argument between those supportive (hereafter referred to as the Yes case) and those unsupportive of compulsory coding (hereafter referred to as the No case). But more than simply produce a list of arguments, this discussion contributes to our understanding of this reception by identifying the ‘discourses’ deployed by both cases (namely, digital ubiquity, disadvantage, and habits of mind discourses) and by providing theoretical framings through which these discourses and their potential implications might be differently understood. Using critical discourse analysis to unpack these discourses shows that while both cases hold to key tenets of liberal-humanism, a commitment to the individual subject, liberty and full participation in the social, it is the Yes case with its stronger commitment to children engaging in abstraction that seems to challenge these. Discussion of this difference is framed by the work of Baudrillard around abstraction, not to ‘prove’ the validity of Baudrillard’s thesis concerning the consequences of humanity’s deepening engagement with abstraction, but to provide a broader understanding of this debate, in relation to a trajectory of engagement with abstraction that seems set to intensify.