bluuebunny/crossref_metadata_2025 huggingface dataset:
Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas
Authors: Vincent-Hubert Françoise , Wacrenier Candice , Morga Benjamin , Lozach Solen , Quenot Emmanuelle , Mège Mickaël , Lecadet Cyrielle , Gourmelon Michèle , Hervio-Heath Dominique , Le Guyader Françoise S.
Published: February 2021

The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus,Vibriospp. and the speciesVibrio alginolyticus,V. cholerae,V. vulnificus, andV. parahaemolyticus, OsHV-1 virus, and bacterial markers of fecal contamination. NoV GII,Vibriospp., and the AllBac generalBacteroidalesmarker were quantified on the three types of membrane. NoV GII and OsHV-1 viruses followed a seasonal distribution. All membranes were favorable for NoV GII detection, while Zetapor was more adapted for OsHV-1 detection. Nylon was more adapted for detection ofVibriospp. and the AllBac marker. The quantities of NoV GII, AllBac, andVibriospp. recovered on membranes increased with the duration of exposure. This first application of passive sampling in seawater is particularly promising in terms of an early warning system for the prevention of contamination in oyster farming areas and to improve our knowledge on the timing and frequency of disease occurence.
Venlafaxine Attenuated the Cognitive and Memory Deficit in Mice Exposed to Isoflurane Alone
Authors: Li Liang , Zhang Chunhai
Published: February 2021

Post-operative cognitive dysfunction (POCD) is a common complication during the post-operative period. It affects the recovery time of the patient after surgery and the stay time in hospital, which causes a great deal of burden to patients and families emotionally and financially. However, there is no specific and effective treatment available for this disorder. Recent study indicated exposure to general anesthetics contributed to POCD by triggering gamma-amino butyric acid type A (GABAA) receptors hyperactivities that persisted even the anesthetic compounds have been eliminated. Here, we investigated the antidepressant, venlafaxine (VLX), in a mouse model of POCD and studied whether VLX attenuated the cognitive dysfunction of mice exposed to general anesthetic, isoflurane (ISO). We found that ISO significantly induced an increased surface expression of the GABAA receptor subunit, α5, in the hippocampus of the mice. However, VLX treatment reduced the increase in α5 subunit expression. Meanwhile, we found the expression levels of interleukin (IL)-1β, tumor necrosis factor alpha (TNF-α), and IL-6 in the brains of mice exposed to ISO were significantly increased. However, VLX could prevent the increase in these cytokines. We also investigated the memory deficit of these mice by using a Y maze behavioral test. Mice with ISO exposure showed decreased alternation performance that could be prevented by the VLX treatment. Collectively, our results here are in line with the previous findings that α5 subunit plays an important role of the formation of POCD, but VLX may be a promising candidate compound for the treatment of POCD.
Frequency and Predictors of Traumatic Encephalopathy Syndrome in a Prospective Cohort of Retired Professional Athletes
Authors: Schaffert Jeff , Didehbani Nyaz , LoBue Christian , Hart John , Rossetti Heidi , Lacritz Laura , Cullum C. Munro
Published: February 2021

Traumatic encephalopathy syndrome (TES) is proposed to represent the long-term impact of repetitive head-injury exposure and the clinical manifestation of chronic traumatic encephalopathy (CTE). This study aimed to evaluate the frequency of TES in a cohort of retired professional contact sport athletes, compare the frequency of TES to clinical consensus diagnoses, and identify predictors that increase the likelihood of TES diagnosis. Participants were 85 retired professional contact sport athletes from a prospective cohort at the University of Texas Southwestern Medical Center and the University of Texas at Dallas. Participants ranged in age from 23 to 79 (M = 55.95, SD = 13.82) and obtained 7 to 19 years of education (M = 16.08, SD = 1.03). Retirees were either non-Hispanic white (n= 62) or African-American (n= 23). Retired athletes underwent a standard clinical evaluation, which included a clinical interview, neurological exam, neuroimaging, neuropsychological testing, and consensus diagnosis of normal, mild cognitive impairment, or dementia. TES criteria were applied to all 85 athletes, and frequencies of diagnoses were compared. Fourteen predictors of TES diagnosis were evaluated using binary logistic regressions, and included demographic, neuropsychological, depression symptoms, and head-injury exposure variables. A high frequency (56%) of TES was observed among this cohort of retired athletes, but 54% of those meeting criteria for TES were diagnosed as cognitively normal via consensus diagnosis. Games played in the National Football League (OR = 0.993,p= 0.087), number of concussions (OR = 1.020,p= 0.532), number of concussions with loss of consciousness (OR = 1.141p= 0.188), and years playing professionally (OR = 0.976,p= 0.627) were not associated with TES diagnosis. Degree of depressive symptomatology, as measured by the total score on the Beck Depression Inventory-II, was the only predictor of TES diagnosis (OR = 1.297,p< 0.001). Our results add to previous findings underscoring the risk for false positive diagnosis, highlight the limitations of the TES criteria in clinical and research settings, and question the relationship between TES and head-injury exposure. Future research is needed to examine depression in retired professional athletes.
Case Report: Catheter-Directed Endovascular Thrombolysis for Refractory Cerebral Venous Sinus Thrombosis in a Patient With Behçet Disease
Authors: Xu Yongfeng , Qiu Minjian
Published: February 2021

Background: Behçet disease (BD) is an autoimmune and vasculitic disorder. BD affects the veins more frequently than the arteries. The cerebral venous sinus thrombosis (CVST) was reported to occur in about 20% of neuro-BD. Huge dilemma exists in the treatment of CVST with BD, some of which were refractory to the standard therapy.

Case Presentation: Here, we report a BD case with refractory intracranial hypertension caused by CVST which is successfully treated with catheter-directed endovascular thrombolysis. Before endovascular thrombolysis, intravenous pulse methylprednisolone combined with anticoagulant therapy was used, and resulted in limited effects.

Conclusion: We conclude that catheter-directed thrombolysis may be considered for refractory CVST with BD, provided that coexistent pulmonary and cerebral artery aneurysms are ruled out.
Development and Validation of a Dynamic Nomogram to Predict the Risk of Neonatal White Matter Damage
Authors: Cao Wenjun , Luo Chenghan , Lei Mengyuan , Shen Min , Ding Wenqian , Wang Mengmeng , Song Min , Ge Jian , Zhang Qian
Published: February 2021

Purpose

White matter damage (WMD) was defined as the appearance of rough and uneven echo enhancement in the white matter around the ventricle. The aim of this study was to develop and validate a risk prediction model for neonatal WMD.

Materials and Methods

We collected data for 1,733 infants hospitalized at the Department of Neonatology at The First Affiliated Hospital of Zhengzhou University from 2017 to 2020. Infants were randomly assigned to training (n= 1,216) or validation (n= 517) cohorts at a ratio of 7:3. Multivariate logistic regression and least absolute shrinkage and selection operator (LASSO) regression analyses were used to establish a risk prediction model and web-based risk calculator based on the training cohort data. The predictive accuracy of the model was verified in the validation cohort.

Results

We identified four variables as independent risk factors for brain WMD in neonates by multivariate logistic regression and LASSO analysis, including gestational age, fetal distress, prelabor rupture of membranes, and use of corticosteroids. These were used to establish a risk prediction nomogram and web-based calculator (https://caowenjun.shinyapps.io/dynnomapp/). The C-index of the training and validation sets was 0.898 (95% confidence interval: 0.8745–0.9215) and 0.887 (95% confidence interval: 0.8478–0.9262), respectively. Decision tree analysis showed that the model was highly effective in the threshold range of 1–61%. The sensitivity and specificity of the model were 82.5 and 81.7%, respectively, and the cutoff value was 0.099.

Conclusion

This is the first study describing the use of a nomogram and web-based calculator to predict the risk of WMD in neonates. The web-based calculator increases the applicability of the predictive model and is a convenient tool for doctors at primary hospitals and outpatient clinics, family doctors, and even parents to identify high-risk births early on and implementing appropriate interventions while avoiding excessive treatment of low-risk patients.
Gut Microbial Profile Is Associated With Residential Settings and Not Nutritional Status in Adults in Karnataka, India
Authors: Mehta Ojasvi , Inbaraj Leeberk Raja , Astbury Stuart , Grove Jane I. , Norman Gift , Aithal Guruprasad P. , Valdes Ana M. , Vijay Amrita
Published: February 2021

Undernutrition is a leading contributor to disease and disability in people of all ages. Several studies have reported significant association between nutritional status and gut microbiome composition but other factors such as demographic settings may also influence the adult microbiome. The relationship between undernourishment and gut microbiome in adults has not been described to date. In this study, we compared the gut microbiome in fecal samples of 48 individuals, from two demographic settings (rural and urban slum) in Karnataka, India using 16S rRNA sequencing. Nutritional status was assessed based on BMI, with a BMI of < 18.5 kg/m2 classified as undernourished, and a BMI in the range 18.5–25 kg/m2 as nourished. We analyzed 25 individuals from rural settings (12 undernourished and 13 nourished) and 23 individuals from urban slum settings (11 undernourished and 12 nourished). We found no significant difference in overall gut microbial diversity (Shannon and Unweighted UniFrac) between undernourished and nourished individuals in either geographical settings, however, microbial taxa at the phylum level (i.e., Firmicutes and Proteobacteria) and beta diversity (unweighted UniFrac) differed significantly between the rural and urban slum settings. By predicting microbial function from 16S data profiling we found significant differences in metabolic pathways present in the gut microbiota from people residing in different settings; specifically, those related to carbohydrate and lipid metabolism. The weighted sum of the KEGG Orthologs associated with carbohydrate metabolism (Spearman's correlation coefficient, ρ = −0.707, p < 0.001), lipid metabolism (Spearman's correlation coefficient, ρ = −0.330, p < 0.022) and biosynthesis of secondary metabolites (Spearman's correlation coefficient, ρ = −0.507, p < 0.001) were decreased in the urban slum group compared to the rural group. In conclusion, we report that the geographical location of residence is associated with differences in gut microbiome composition in adults. We found no significant differences in microbiome composition between nourished and undernourished adults from urban slum or rural settings in India.
Emerging Role of Consolidative Radiotherapy After Complete Remission Following R-CHOP Immunochemotherapy in Stage III–IV Diffuse Large B-Cell Lymphoma: A Single Institutional and Case-Matched Control Study
Authors: Hong Ji Hyun , Lee Han Hee , Jung Seung-Eun , Park Gyeongsin , O Joo-Hyun , Jeon Young-Woo , Choi Byung-Ock , Cho Seok-Goo
Published: February 2021

Purpose

The role of consolidative radiotherapy (RT) after complete-remission (CR) following rituximab combined with cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) in advanced-stage diffuse large B-cell lymphoma (DLBCL) remains unclear. We retrospectively analyzed the survival outcomes and patterns of failure with our institutional experience.

Material and Methods

Between 2009 and 2018, 206 patients with stage III-IV DLBCL achieved CR after receiving R-CHOP. Propensity-score matching was used to analyze the role of consolidative RT. The consolidative RT group (n = 34) and the R-CHOP alone group (n = 68) were matched at a 1:2 ratio. After propensity-score matching, 102 patients were analyzed.

Results

With a median follow-up of 39.7 months, 26 patients (25.5%) showed local recurrence. Only one patient failed at the previous RT field. RT was delivered to bulky sites, head and neck lesions, testes, and bone with median dose of 30.6 Gy. The most common site of failure was head and neck lesions followed by bulky sites. The 5-year overall survival (OS), progression-free survival (PFS), and isolated-local recurrence free survival (LRFS) were 73.5, 64.0, and 79.9%. In univariate and multivariate analysis, bone marrow involvement and consolidative RT were associated with isolated LRFS (p = 0.006 and 0.032) significantly.

Conclusion

Consolidative RT improved isolated local control. Based on the pattern of failure, we carefully suggest to radiate on initially involved bulky sites or head and neck lesions. Further studies need to be done to find out the optimal radiation dose and selection of RT site.
Diagnostic Utility of IGF2BP1 and Its Targets as Potential Biomarkers in ETV6-RUNX1 Positive B-Cell Acute Lymphoblastic Leukemia
Authors: Sharma Gunjan , Boby Elza , Nidhi Thakur , Jain Ayushi , Singh Jay , Singh Archna , Chattopadhyay Parthaprasad , Bakhshi Sameer , Chopra Anita , Palanichamy Jayanth Kumar
Published: February 2021

Around 85% of childhood Acute Lymphoblastic Leukemia (ALL) are of B-cell origin and characterized by the presence of different translocations including BCR-ABL1, ETV6-RUNX1, E2A-PBX1, and MLL fusion proteins. The current clinical investigations used to identify ETV6-RUNX1 translocation include FISH and fusion transcript specific PCR. In the current study we assessed the utility of IGF2BP1, an oncofetal RNA binding protein, that is over expressed specifically in ETV6-RUNX1 translocation positive B-ALL to be used as a diagnostic marker in the clinic. Further, public transcriptomic and Crosslinked Immunoprecipitation (CLIP) datasets were analyzed to identify the putative targets of IGF2BP1. We also studied the utility of using the mRNA expression of two such targets, MYC and EGFL7 as potential diagnostic markers separately or in conjunction with IGF2BP1. We observed that the expression of IGF2BP1 alone measured by RT-qPCR is highly sensitive and specific to be used as a potential biomarker for the presence of ETV6-RUNX1 translocation in future.
Validation of the GenesWell BCT Score in Young Asian Women With HR+/HER2− Early Breast Cancer
Authors: Kwon Mi Jeong , Ryu Jai Min , Cho Soo Youn , Nam Seok Jin , Kim Seok Won , Lee Jeeyeon , Lee Soo Jung , Park Ji-Young , Park Ho Yong , Hong Sungjun , Kim Kyunga , Han Jinil , Moon Youngho , Shin Young Kee , Lee Jeong Eon
Published: February 2021

Background

The prognostic or predictive value of commonly used multigene assays in young patients with hormone receptor-positive (HR+), human epidermal growth factor receptor 2-negative (HER2−) early breast cancer is unclear. In this study, we assessed the prognostic value of the GenesWell BCT assay according to age group.

Methods

We identified patients with pN0-1, HR+/HER2− breast cancer in a prospective cohort of women who underwent surgery between 2005 and 2017. The GenesWell BCT assay was performed on tissue samples from selected patients. Distant metastasis-free survival (DMFS) and disease-free survival (DFS) were compared between the risk groups assigned by the BCT score.

Results

A total of 712 patients were eligible for analysis. The median follow-up time was 7.47 years. The BCT score was prognostic in patients aged ≤50 years (n = 404) and those aged >50 years (n = 308). In both age groups, the 10-year DMFS and DFS rates for patients classified as high risk by the BCT score were significantly lower than those for patients classified as low risk. A multivariate analysis revealed that the BCT score was an independent prognostic factor for DFS in patients aged ≤50 years (hazard ratio, 1.28; 95% CI, 1.05–1.56; P = 0.015), as well as those aged >50 years.

Conclusion

The BCT score could be used to identify low-risk patients who will not benefit from adjuvant chemotherapy to treat HR+/HER2− early breast cancer regardless of age. A further prospective study to assess the prognostic and predictive value of the BCT score is required.
Dysbiosis of Oral Microbiota During Oral Squamous Cell Carcinoma Development
Authors: Sarkar Purandar , Malik Samaresh , Laha Sayantan , Das Shantanab , Bunk Soumya , Ray Jay Gopal , Chatterjee Raghunath , Saha Abhik
Published: February 2021

Infection with specific pathogens and alterations in tissue commensal microbial composition are intricately associated with the development of many human cancers. Likewise, dysbiosis of oral microbiome was also shown to play critical role in the initiation as well as progression of oral cancer. However, there are no reports portraying changes in oral microbial community in the patients of Indian subcontinent, which has the highest incidence of oral cancer per year, globally. To establish the association of bacterial dysbiosis and oral squamous cell carcinoma (OSCC) among the Indian population, malignant lesions and anatomically matched adjacent normal tissues were obtained from fifty well-differentiated OSCC patients and analyzed using 16S rRNA V3-V4 amplicon based sequencing on the MiSeq platform. Interestingly, in contrast to the previous studies, a significantly lower bacterial diversity was observed in the malignant samples as compared to the normal counterpart. Overall our study identified Prevotella, Corynebacterium, Pseudomonas, Deinococcus and Noviherbaspirillum as significantly enriched genera, whereas genera including Actinomyces, Sutterella, Stenotrophomonas, Anoxybacillus, and Serratia were notably decreased in the OSCC lesions. Moreover, we demonstrated HPV-16 but not HPV-18 was significantly associated with the OSCC development. In future, with additional validation, this panel could directly be applied into clinical diagnostic and prognostic workflows for OSCC in Indian scenario.
Huaier Extract Inhibits Prostate Cancer Growth via Targeting AR/AR-V7 Pathway
Authors: Liu Zhengfang , Liu Cheng , Yan Keqiang , Liu Jikai , Fang Zhiqing , Fan Yidong
Published: February 2021

The androgen receptor (AR) plays a pivotal role in prostatic carcinogenesis, and it also affects the transition from hormone sensitive prostate cancer (HSPC) to castration-resistant prostate cancer (CRPC). Particularly, the persistent activation of the androgen receptor and the appearance of androgen receptor splicing variant 7 (AR-V7), could partly explain the failure of androgen deprivation therapy (ADT). In the present study, we reported that huaier extract, derived from officinal fungi, has potent antiproliferative effects in both HSPC and CRPC cells. Mechanistically, huaier extract downregulated both full length AR (AR-FL) and AR-V7 mRNA levels via targeting the SET and MYND domain-containing protein 3 (SMYD3) signaling pathway. Huaier extract also enhanced proteasome-mediated protein degradation of AR-FL and AR-V7 by downregulating proteasome-associated deubiquitinase ubiquitin-specific protease 14 (USP14). Furthermore, huaier extract inhibited AR-FL/AR-V7 transcriptional activity and their nuclear translocation. More importantly, our data demonstrated that huaier extract could re-sensitize enzalutamide-resistant prostate cancer cells to enzalutamide treatment in vitro and in vivo models. Our work revealed that huaier extract could be effective for treatment of prostate cancer either as monotherapy or in combination with enzalutamide.
The Potential Therapeutic Effect of RNA Interference and Natural Products on COVID-19: A Review of the Coronaviruses Infection
Authors: Kalhori Mohammad Reza , Saadatpour Fatemeh , Arefian Ehsan , Soleimani Masoud , Farzaei Mohammad Hosien , Aneva Ina Yosifova , Echeverría Javier
Published: February 2021

The SARS-CoV-2 virus was reported for the first time in Wuhan, Hubei Province, China, and causes respiratory infection. This pandemic pneumonia killed about 1,437,835 people out of 61,308,161cases up to November 27, 2020. The disease’s main clinical complications include fever, recurrent coughing, shortness of breath, acute respiratory syndrome, and failure of vital organs that could lead to death. It has been shown that natural compounds with antioxidant, anticancer, and antiviral activities and RNA interference agents could play an essential role in preventing or treating coronavirus infection by inhibiting the expression of crucial virus genes. This study aims to introduce a summary of coronavirus’s genetic and morphological structure and determine the role of miRNAs, siRNAs, chemical drugs, and natural compounds in stimulating the immune system or inhibiting the virus’s structural and non-structural genes that are essential for replication and infection of SARS-CoV-2.
Anticancer Properties and Mechanisms of Singly-Protonated Dehydronorcantharidin Silver Coordination Polymer in a Bladder Cancer Model
Authors: Zhou Changkuo , Wang Ganyu , Jing Weiqiang , Tan Xuejie , Guo Hu
Published: February 2021

Bladder cancer is the most common malignant urinary system tumor. Chemotherapy is frequently used as a treatment regimen for patients with bladder cancer, however, new and effective drugs for bladder cancer need to be developed. The present study examined the effects and mechanisms of Ag-SP-DNC, a silver and singly-protonated dehydronorcantharidin complex, on bladder cancer in vitro and in vivo. It was identified that Ag-SP-DNC suppressed cell proliferation and induced apoptosis in bladder cancer cells in vitro, a suppression associated with G0/G1 phase arrest and elevated intracellular reactive oxygen species (ROS) levels. Furthermore, Ag-SP-DNC enhanced the cleaved caspase-3 levels, disrupted the mitochondrial transmembrane potential balance, and induced intracellular calcium overload. The Ag-SP-DNC-induced bladder cancer cell apoptosis was significantly decreased following treatment with a broad caspase inhibitor, zVAD-fmk. In addition, treatment of MB49 tumor-bearing mice with Ag-SP-DNC significantly inhibited tumor growth and decreased the anti-apoptosis and cell cycle promotion protein levels in the tumor. The results of the present study suggested that Ag-SP-DNC elicits a strong anticancer effect against bladder cancer, and can therefore be used as a promising treatment for bladder cancer.
Ginsenoside Rc Ameliorates Endothelial Insulin Resistance via Upregulation of Angiotensin-Converting Enzyme 2
Authors: Wang Yaozhen , Fu Wenwen , Xue Yan , Lu Zeyuan , Li Yuangeng , Yu Ping , Yu Xiaofeng , Xu Huali , Sui Dayun
Published: February 2021

Type 2 diabetes mellitus (T2DM) is a major health concern which may cause cardiovascular complications. Insulin resistance (IR), regarded as a hallmark of T2DM, is characterized by endothelial dysfunction. Ginsenoside Rc is one of the main protopanaxadiol-type saponins with relatively less research on it. Despite researches confirming the potent anti-inflammatory and antioxidant activities of ginsenoside Rc, the potential benefits of ginsenoside Rc against vascular complications have not been explored. In the present study, we investigated the effects of ginsenoside Rc on endothelial IR and endothelial dysfunction with its underlying mechanisms using high glucose- (HG-) cultured human umbilical vein endothelial cells (HUVECs) in vitro and a type 2 diabetic model of db/db mice in vivo. The results showed that ginsenoside Rc corrected the imbalance of vasomotor factors, reduced the production of Ang (angiotensin) II, and activated angiotensin-converting enzyme 2 (ACE2)/Ang-(1–7)/Mas axis in HG-treated HUVECs. Besides, ginsenoside Rc improved the impaired insulin signaling pathway and repressed oxidative stress and inflammatory pathways which constitute key factors leading to IR. Interestingly, the effects of ginsenoside Rc on HG-induced HUVECs were abolished by the selective ACE2 inhibitor MLN-4760. Furthermore, ginsenoside Rc exhibited anti-inflammatory as well as antioxidant properties and ameliorated endothelial dysfunction via upregulation of ACE2 in db/db mice, which were confirmed by the application of MLN-4760. In conclusion, our findings reveal a novel action of ginsenoside Rc and demonstrate that ginsenoside Rc ameliorated endothelial IR and endothelial dysfunction, at least in part, via upregulation of ACE2 and holds promise for the treatment of diabetic vascular complications.
Deformation Parameters and Collective Temperature Changes in Photofission Mass Yields of Actinides Within the Systematic Statistical Scission Point Model
Authors: Kadiani P. Mehdipour
Published: February 2021

The photofission fragment mass yields of actinides are evaluated using a systematic statistical scission point model. In this model, all energies at the scission point are presented as a linear function of the mass numbers of fission fragments. The mass yields are calculated with a new approximated relative probability for each complementary fragment. The agreement with the experimental data is quite good, especially with a collective temperature Tcol of 2 MeV at intermediate excitation energy and Tcol = 1 MeV for spontaneous fission. This indicates that the collective temperature is greater than the value obtained by the initial excitation energy. The generalized superfluid model is applied for calculating the fragment temperature. The deformation parameters of fission fragments have been obtained by fitting the calculated results with the experimental values. This indicates that the deformation parameters decrease with increasing excitation energy. Also, these parameters decrease for fissioning systems with odd mass numbers.
Estimating Central Pulse Pressure From Blood Flow by Identifying the Main Physical Determinants of Pulse Pressure Amplification
Authors: Flores Gerónimo Joaquín , Corvera Poiré Eugenia , Chowienczyk Philip , Alastruey Jordi
Published: February 2021

Several studies suggest that central (aortic) blood pressure (cBP) is a better marker of cardiovascular disease risk than peripheral blood pressure (pBP). The morphology of the pBP wave, usually assessed non-invasively in the arm, differs significantly from the cBP wave, whose direct measurement is highly invasive. In particular, pulse pressure, PP (the amplitude of the pressure wave), increases from central to peripheral arteries, leading to the so-called pulse pressure amplification (ΔPP). The main purpose of this study was to develop a methodology for estimating central PP (cPP) from non-invasive measurements of aortic flow and peripheral PP. Our novel approach is based on a comprehensive understanding of the main cardiovascular properties that determine ΔPP along the aortic-brachial arterial path, namely brachial flow wave morphology in late systole, and vessel radius and distance along this arterial path. This understanding was achieved by using a blood flow model which allows for workable analytical solutions in the frequency domain that can be decoupled and simplified for each arterial segment. Results show the ability of our methodology to (i) capture changes in cPP and ΔPP produced by variations in cardiovascular properties and (ii) estimate cPP with mean differences smaller than 3.3 ± 2.8 mmHg on in silico data for different age groups (25–75 years old) and 5.1 ± 6.9 mmHg on in vivo data for normotensive and hypertensive subjects. Our approach could improve cardiovascular function assessment in clinical cohorts for which aortic flow wave data is available.
Construction of a High-Density Genetic Map of Acca sellowiana (Berg.) Burret, an Outcrossing Species, Based on Two Connected Mapping Populations
Authors: Quezada Marianella , Amadeu Rodrigo Rampazo , Vignale Beatriz , Cabrera Danilo , Pritsch Clara , Garcia Antonio Augusto Franco
Published: February 2021

Acca sellowiana, known as feijoa or pineapple guava, is a diploid, (2n = 2x = 22) outcrossing fruit tree species native to Uruguay and Brazil. The species stands out for its highly aromatic fruits, with nutraceutical and therapeutic value. Despite its promising agronomical value, genetic studies on this species are limited. Linkage genetic maps are valuable tools for genetic and genomic studies, and constitute essential tools in breeding programs to support the development of molecular breeding strategies. A high-density composite genetic linkage map of A. sellowiana was constructed using two genetically connected populations: H5 (TCO × BR, N = 160) and H6 (TCO × DP, N = 184). Genotyping by sequencing (GBS) approach was successfully applied for developing single nucleotide polymorphism (SNP) markers. A total of 4,921 SNP markers were identified using the reference genome of the closely related species Eucalyptus grandis, whereas other 4,656 SNPs were discovered using a de novo pipeline. The individual H5 and H6 maps comprised 1,236 and 1,302 markers distributed over the expected 11 linkage groups, respectively. These two maps spanned a map length of 1,593 and 1,572 cM, with an average inter-marker distance of 1.29 and 1.21 cM, respectively. A large proportion of markers were common to both maps and showed a high degree of collinearity. The composite map consisted of 1,897 SNPs markers with a total map length of 1,314 cM and an average inter-marker distance of 0.69. A novel approach for the construction of composite maps where the meiosis information of individuals of two connected populations is captured in a single estimator is described. A high-density, accurate composite map based on a consensus ordering of markers provides a valuable contribution for future genetic research and breeding efforts in A. sellowiana. A novel mapping approach based on an estimation of multipopulation recombination fraction described here may be applied in the construction of dense composite genetic maps for any other outcrossing diploid species.
The Search for and Presence of Calling: Latent Profiles and Relationships With Work Meaning and Job Satisfaction
Authors: Li Feifei , Jiao Runkai , Liu Dan , Yin Hang
Published: February 2021

Previous studies showed inconsistent results on the association between searching for calling and its psychosocial functioning outcomes (i.e., work meaning and job satisfaction). The link of searching for calling to its psychosocial functioning outcomes may be influenced by the presence of calling because the search for and presence of calling can co-exist within individuals. Thus, the present study employed a person-centered method (latent profile analysis) to identify subgroups combining the search for and presence of a calling and then explore the identified profiles' differences in work meaning and job satisfaction. Study participants were Chinese kindergarten teachers (n = 726). Latent profile analysis revealed four different groups: (1) actively maintaining calling (24.93%), (2) unsustainable calling (11.43%), (3) moderately increasing calling (23.14%), and (4) actively increasing calling (40.50%). Subsequent analyses showed notable differences across the four groups on work meaning and job satisfaction. Participants in profile 1 with both the highest searching for and presence of calling would experience more work meaning and job satisfaction than those in the other profiles whose strengths of searching for and presence of calling were relatively low. Participants in profile 4 had higher searching for and presence of calling than those in profile 3, and they experienced more meaningfulness at work and were more satisfied with their job. These findings indicate that actively searching for calling is closely associated with more work meaning and job satisfaction among people who already perceive intensive calling. Implications, limitations, and future directions of the results are discussed.
The Influence of Trait Compulsivity and Impulsivity on Addictive and Compulsive Behaviors During COVID-19
Authors: Albertella Lucy , Rotaru Kristian , Christensen Erynn , Lowe Amelia , Brierley Mary-Ellen , Richardson Karyn , Chamberlain Samuel R. , Lee Rico S. C. , Kayayan Edouard , Grant Jon E. , Schluter-Hughes Sam , Ince Campbell , Fontenelle Leonardo F. , Segrave Rebecca , Yücel Murat
Published: February 2021

Background: The COVID-19 pandemic has resulted in high levels of psychological distress worldwide, with experts expressing concern that this could result in corresponding increases in addictive behaviors as individuals seek to cope with their distress. Further, some individuals may be at greater risk than others for developing problematic addictive behaviors during times of high stress, such as individuals with high trait impulsivity and compulsivity. Despite the potential of such knowledge to inform early detection of risk, no study to date has examined the influence of trait impulsivity and compulsivity on addictive behaviors during COVID-19. Toward this aim, the current study examined the association between impulsive and compulsive traits and problematic addictive and compulsive behaviors during the first COVID-19 lockdown in Australia.

Methods: Eight hundred seventy-eight adults completed a cross-sectional online survey during the first lockdown, between late May to June 2020. Participants completed scales for addictive and compulsive behaviors for the period prior to and during lockdown for problematic eating, pornography, internet use, gambling, drinking, and obsessive-compulsive behaviors. Negative binomial regressions examined the associations between impulsivity, compulsivity, and their interaction with problematic behaviors during lockdown, controlling for age, gender, sample, psychological distress, exposure to COVID-related stressors, and pre-COVID problems.

Results: Greater trait compulsivity was associated with more problematic obsessive-compulsive behaviors (p < 0.001) and less problematic drinking (p = 0.038) during lockdown. Further, trait compulsivity interacted with trait impulsivity in relation to problematic eating behaviors (p = 0.014) such that greater trait compulsivity was associated with more problems among individuals with low impulsivity only (p = 0.030). Finally, psychological distress and/or exposure to COVID-related stressors were associated with greater problems across all addictive and compulsive behaviors, as was severity of pre-COVID problems.

Discussion: Trait compulsivity was associated with addictive and compulsive behaviors in different ways. Further, the finding that stress-related variables (psychological distress and COVID-related stressors) were associated with greater problems across all lockdown behaviors supports the idea that stress may facilitate, or otherwise be associated with, problematic behaviors. These findings highlight the need for interventions that enhance resilience to stress, which in turn may reduce risk for addictive and compulsive disorders.
On Cellular Networks Supporting Healthcare Remote Monitoring in IoT Scenarios
Authors: Petroni Andrea , Salvo Pierpaolo , Cuomo Francesca
Published: February 2021

In the next few years, fundamental technological transitions are expected both for wireless communications, soon resulting in the 5G era, and for the kind of pervasiveness that will be achieved thanks to the Internet of Things. The implementation of such new communication paradigms is expected to significantly revolutionize people’s lives, industry, commerce, and many daily activities. Healthcare applications are considered to be one of the most impacted industries. Sadly, in relation to the COVID-19 pandemic currently afflicting our society, health remote monitoring has become a fundamental and urgent application. The overcrowding of hospitals and medical facilities due to COVID-19, has unavoidably created delays and key issues in providing adequate medical assistance. In several cases, asymptomatic or light symptomatic COVID-19 patients have to be continuously monitored to prevent emergencies, and such an activity does not necessarily require hospitalization. Considering this research direction, this paper investigates the potentiality of cloud-based cellular networks to support remote healthcare monitoring applications implemented in accordance with the IoT paradigm, combined with future cellular systems. The idea is to conveniently replace the physical interaction between patients and doctors with a reliable virtual one, so that hospital services can be reserved for emergencies. Specifically, we investigate the feasibility and effectiveness of remote healthcare monitoring by evaluating its impact on the network performance. Furthermore, we discuss the potentiality of medical data compression and how it can be exploited to reduce the traffic load.
Fitness and Performance Testing of Male and Female Beach Soccer Players—A Preliminary Investigation
Authors: Larsen Malte N. , Ermidis Georgios , Brito João , Ørner Cecilie , Martins Clarice , Lemos Luís Filipe , Krustrup Peter , Rago Vincenzo
Published: February 2021

Purpose: This study aimed to compare performance on sand and a firm surface and to describe the physical capacity of male and female beach soccer players.

Methods: Sixty-six male and 29 female competitive beach soccer players voluntarily participated in this study. Firstly, within-subjects test scores were compared to scores on a firm surface (criterion validity; n = 15 men) and reconducted on a second occasion (reliability; n = 51 men). Secondly, the best score on sand was retained to compare test performance between ages (classified as below 20, 20–30, and above 30 years) and sexes. Performance assessments included sprint time over 5 and 15 m (once on a firm surface and twice on sand), standing long jump (SLJ, once on a firm surface and twice on sand) and Yo-Yo intermittent recovery level 1 (Yo-Yo IR1, once on a firm surface and once on sand; only data for men were available).

Results: Five-m sprint and Yo-Yo IR1 performance on sand were not correlated to performance on a firm surface (P > 0.05). Test-retest reliability was acceptable for the 15-m sprint and SLJ tests (ICC > 0.90; CV < 5%). Performance in 15-m sprint and maximal sprinting speed were moderately lower in male players aged above 30 years. compared to players aged below 30 years (d = 0.35–0.42; P < 0.05). Irrespective of the age group, weight-bearing power-based performance mass was moderately to very largely higher in male players than in female players (d = 0.42–0.88; P < 0.05).

Conclusions: The lack of a consistent relationship between performance on sand and on a firm surface might indicate the need to develop specific test batteries for sand-based athletes. Age-related differences in physical performance were evident only in sprint capacity. Further studies are warranted to elucidate our preliminary findings and to develop the sand specific tests.
Mapping Priority Areas for Apiculture Development with the Use of Geographical Information Systems
Authors: Marnasidis Simeon , Kantartzis Apostolos , Malesios Chrisovalantis , Hatjina Fani , Arabatzis Garyfallos , Verikouki Efstathia
Published: February 2021

Supporting local and central authorities in decision-making processes pertaining to environmental planning requires the adoption of scientific methods and the submission of proposals that could be implemented in practice. Taking into consideration the dual role that honeybees play as honey producers and crop pollinators, the aim of the present study is to identify and utilize a number of indicators and subsequently develop priority thematic maps. Previous research has focused on the determination of, and, on certain occasions, on mapping, priority areas for apiculture development, based mainly on the needs of honeybees, without taking into consideration the pollination needs of crops that are cultivated in these areas. In addition, research so far has been carried out in specific spatial entities, in contrast to the current study, in which the areas to be comparatively assessed are pre-chosen based on their geographical boundaries. The information derived from this process is expected to help decision-makers in local and regional authorities to adopt measures for optimal land use and sound pollination practices in order to enhance apiculture development at a local scale. To achieve this target, the study incorporates literature about the attractiveness of crops and plants to pollinating honeybees as well as the pollination services provided by honeybees, in combination with detailed vegetative land cover data. The local communities of each municipality were comparatively evaluated, by introducing three indicators through numerical and spatial data analysis: Relative Attractiveness Index (RAI), Relative Dependence Index (RDI), and Relative Priority Index (RPI). Based on these indicators, attractiveness, dependence, and priority maps were created and explained in detail. We suggest that a number of improvement measures that will boost pollination or honey production or both should be taken by decision-makers, based on the correlations between the aforementioned indicators and the exanimated areas. In addition, dependence maps can constitute a powerful tool for raising awareness among both the public and the farmers about the value of honeybees in pollination, thus reinforcing bee protection efforts undertaken globally. Attractiveness maps that provide a thorough picture of the areas that are sources of pollen and nectar can serve as a general guide for the establishment of hives in areas with high potential for beekeeping.
Cladosporium sp. Isolate as Fungal Plant Growth Promoting Agent
Authors: Răut Iuliana , Călin Mariana , Capră Luiza , Gurban Ana-Maria , Doni Mihaela , Radu Nicoleta , Jecu Luiza
Published: February 2021

Cladosporium species are active in protecting plants against different biotic and abiotic stresses. Since these species produced a wide range of secondary metabolites responsible for the adaptation to new habitats, plant health and performance, they are of great interest, especially for biostimulants in agriculture. Cladosporium sp. produces protein hydrolysates (PHs), a class of biostimulants, by cultivation on medium with keratin wastes (feathers) as carbon and energy sources. The aim of this study was to select a Cladosporium isolate with potential to be used as plant growth promoting agent. The characteristics of Cladosporium isolates as plants biostimulants were evaluated through several tests, such as: antagonism versus plants pathogens, effect on plant growth of secreted volatiles produced by isolates, secretion of hydrolytic enzymes, production of 3-indole acetic acid, zinc and phosphorous solubilization, capacity to promote tomato seedlings growth (pot experiments). Cladosporium isolate T2 presented positive results to all tests. Encouraging results were obtained treating tomato seedlings with PHs from isolate Cladosporium T2 cultured on medium supplemented with 1% (w/w) chicken feathers, for which growth parameters, such as stem weight, stem height, and root weight were significantly higher by 65%, 32%, and 55%, respectively, compared to those treated with water.
Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry
Authors: Titova Maria V. , Popova Elena V. , Konstantinova Svetlana V. , Kochkin Dmitry V. , Ivanov Igor M. , Klyushin Andrey G. , Titova Elena G. , Nebera Elena A. , Vasilevskaya Ekaterina R. , Tolmacheva Galina S. , Kotenkova Elena A. , Nosov Alexandr M. , Paek Kee-Yoeup
Published: February 2021

Dioscorea deltoidea is a medicinal plant valued for its high content of steroidal glycosides (SG)—bioactive compounds with cardioprotective and immunomodulation actions, also used to treat reproductive system disorders. To overcome the limitations of natural resources of this species, a suspension cell culture of D. deltoidea was developed as a renewable and ecologically sustainable source of raw biomass and SG. Cell culture demonstrated stable and intensive growth in the laboratory (20 L) and industrial (630 L) bioreactors operated under a semi-continuous regime (specific growth rate 0.11–1.12 day−1, growth index 3.5–3.7). Maximum dry weight accumulation (8.5–8.8 g/L) and SG content (47–57 mg/g DW) were recorded during the stationary phase. Bioreactor-produced cell biomass contained inorganic macro (K, Ca, Mg, Na) and micro (Zn, Mn, Fe, B, Al, Cu, Cr, Se, Co, Ni) elements in concentrations within the safe range of dietary recommendations. Acute toxicity test showed no or insignificant changes in organ weight, hematological panel and blood biochemistry of laboratory animals fed with 2000 and 5000 mg/kg dry biomass. The results suggest that cell culture of D. deltoidea grown in bioreactors has great potential to be used as functional foods and a component of specialized dietary supplements in complex therapy of reproductive system disorders and mineral deficiency.
Evaluation of Humic Fertilizers Applied at Full and Reduced Nitrogen Rates on Kentucky Bluegrass Quality and Soil Health
Authors: Lindsey Alex J. , Thoms Adam W. , McDaniel Marshall D. , Christians Nick E.
Published: February 2021

Soil health and sustainable management practices have garnered much interest within the turfgrass industry. Among the many practices that enhance soil health and sustainability are applying soil additives to enhance soil biological activity and reducing nitrogen (N) inputs—complimentary practices. A two-year study was conducted to investigate if reduced N fertilizer rates applied with humic substances could provide comparable turfgrass quality as full N rates, and whether humic fertilizers would increase biological aspects of soil health (i.e., microbial biomass and activity). Treatments included synthetic fertilizer with black gypsum (SFBG), poly-coated humic-coated urea (PCHCU; two rates), urea + humic dispersing granules (HDG; two rates), urea, stabilized nitrogen, HDG, and a nontreated control. Reduced rates of N with humic substances maintained turfgrass quality and cover, and reduced clipping biomass compared to full N rates. There were no differences in soil physical and chemical properties besides soil sulfur (S) concentration. SFBG resulted in the highest soil S concentration. Fertilizer treatments had minimal effect on microbial biomass and other plant-available nutrients. However, PCHCU (full rate) increased potentially mineralizable carbon (PMC) and N (PMN) by 68% and 59%, respectively, compared to the nontreated control. Meanwhile SFBG and stabilized nitrogen also increased PMC and PMN by 77% and 50%, and 65% and 59%, respectively. Overall, applications of reduced N fertilizer rates with the addition of humic substances could be incorporated into a more sustainable and environmentally friendly turfgrass fertilizer program.