bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of 2 different inhaled epoprostenol dosing strategies for acute respiratory distress syndrome in critically ill adults: Weight-based vs fixed-dose administration
Authors: Buckley Mitchell S , Mendez Angel , Radosevich John J , Agarwal Sumit K , MacLaren Robert
Published: July 2022

Purpose

Inhaled epoprostenol (iEPO) is a viable, temporizing option for acute respiratory distress syndrome (ARDS), although the optimal iEPO dosing strategy remains inconclusive. The purpose of this study was to evaluate oxygenation and ventilation parameters in a comparison of weight-based and fixed-dose iEPO in adult patients with moderate-to-severe ARDS.

Methods

A retrospective cohort study was conducted at 2 academic medical centers in adult intensive care unit (ICU) patients administered either fixed-dose or weight-based iEPO for moderate-to-severe ARDS. The primary endpoint was the highest recorded change in the ratio of arterial oxygen partial pressure to fractional inspired oxygen (PaO2/FiO2) within 4 hours of baseline. Secondary analyses compared responder rates within 4 hours of initiation, oxygenation and ventilation parameters, in-hospital mortality rates, mechanical ventilation duration, length of stay (ICU and hospital), and tracheostomy rates between the study groups.

Results

A total of 294 patients were included, n = 194 with 100 (34.0%) and 194 (66.0%) in the weight-based and fixed-dose iEPO groups, respectively. The mean (SD) change in the highest recorded PaO2/FiO2 value from baseline up to 4 hours after initiation in the fixed-dose and weight-based groups was 81.1 (106.0) and 41.0 (72.5) mm Hg, respectively (P = 0.0015). The responder rate at 4 hours after iEPO initiation was significantly higher in the fixed-dose group (69.9%) than in the weight-based group (30.1%) (P = 0.02). The only predictor of response was fixed-dose administration (odds ratio, 3.28; 95% confidence interval, 1.6-6.7; P = 0.0012). Clinical outcomes were comparable between the groups.

Conclusion

Fixed-dose iEPO was associated with significantly higher response rates then weight-based iEPO during the first 4 hours of therapy. Fixed-dose iEPO is a more convenient strategy than weight-based approaches.
Histamine elimination by a coupling reaction of fungal amine oxidase and bacterial aldehyde oxidase
Authors: Usui Masakatsu , Kubota Hikari , Ishihara Mizuki , Matsuki Haruka , Kawabe Shinya , Sugiura Yoshimasa , Kataoka Naoya , Matsushita Kazunobu , Ano Yoshitaka , Akakabe Yoshihiko , Hours Roque A , Yakushi Toshiharu , Adachi Osao
Published: July 2022

Histamine (HIST) and other biogenic amines found in fish and fishery products accumulated by the action of bacterial amino acid decarboxylase cannot be decomposed and eliminated by heating or other chemical methods. A simple method for HIST elimination is proposed by a coupling reaction of the fungal amine oxidase (FAO) and bacterial aldehyde oxidase (ALOX) of acetic acid bacteria. As a model reaction, FAO oxidized benzylamine to benzaldehyde, which in turn was oxidized spontaneously to benzoic acid with ALOX. Likely, in HIST elimination, FAO coupled well with ALOX to produce imidazole 4-acetic acid from HIST with an apparent yield of 100%. Imidazole 4-acetaldehyde was not detected in the reaction mixture. In the absence of ALOX, the coupling reaction was incomplete given a number of unidentified substances in the reaction mixture. The proposed coupling enzymatic method may be highly effective to eliminate toxic amines from fish and fishery products.
A Novel Representation and Prediction Initiative for Underground Water by Using Deep Learning Technique of Remote Sensing Images
Authors: Sureshkumar Veluguri , Somarajadikshitar Rajasomashekar , Beeram B Sarala
Published: July 2022

This paper intends to introduce a novel groundwater prediction model by inducing the novel hydro indices that are not yet popular in earlier techniques. As per the proposed work, statistical features like mean, median, skewness and kurtosis are estimated. Moreover, the vegetation index includes simple ratio, normalized difference vegetation index, Kauth–Thomas Tasseled cap transformation and infrared index transformation. Furthermore, a novel hydro index is formulated by combining the statistical model function with the vegetation index. Subsequently, the detection process is carried out by ensemble technique, which includes the classifiers like random forest (RF), neural network (NN), support vector machine (SVM) and deep belief network (DBN). The final predicted result is attained from DBN. The performance of the adopted model is computed to the existing models with respect to certain measures. At learning rate 50, the maximum accuracy of the proposed model is 45.65, 34.78, 58.70, 72.83, 18.48 and 23.91% better than the existing models like SVM, RF, convolutional neural network, K-nearest neighbors, NN and artificial neural network, respectively.
The Lambeth Articles (1595) and the Doctrinal Stance of the Church of England
Authors: Tyacke Nicholas
Published: July 2022

The Lambeth Articles have always bulked large in debates about the Calvinist tenor of sixteenth-century English Protestantism. But in a recent article, Debora Shuger has raised the stakes dramatically. Not content to deny that the Lambeth Articles were ‘Calvinist’, she claims that they were the work of a group of ‘non-Calvinist divines’, specially commissioned by Archbishop Whitgift to produce a ‘consensus document’ designed to put an end to the predestinarian controversy which had broken out at Cambridge University. Paradoxically, Shuger combines this with the view that the Lambeth Articles were nevertheless suppressed, on the orders of Queen Elizabeth. It follows that the idea of a subsequent ‘rise of English Arminianism’ is fundamentally flawed. The present piece argues that the Lambeth Articles in their final form (a) emanated from a meeting of the Court of High Commission at Lambeth, presided over by Whitgift himself, Bishop Richard Fletcher of London, and Richard Vaughan, the bishop-elect of Bangor, all of them Calvinists; (b) are best understood as speaking the language of sublapsarian Calvinism; and (c) were not suppressed but continued to be regarded as interpretative guidelines until the 1620s. The 1620s, however, witnessed a dramatic renversement when, unlike the 1590s, Calvinism was indeed suppressed by royal command and at the hands of a new breed of anti-Calvinist divines. Nevertheless, via the Irish Articles of 1615, the Lambeth Articles were to attain an afterlife as part of the Westminster Confession of Faith promulgated in 1648.
Premature translation of the Drosophila zygotic genome activator Zelda is not sufficient to precociously activate gene expression
Authors: Larson Elizabeth D , Komori Hideyuki , Fitzpatrick Zoe A , Krabbenhoft Samuel D , Lee Cheng-Yu , Harrison Melissa
Published: July 2022

Following fertilization, the unified germ cells rapidly transition to a totipotent embryo. Maternally deposited mRNAs encode the proteins necessary for this reprogramming as the zygotic genome remains transcriptionally quiescent during the initial stages of development. The transcription factors required to activate the zygotic genome are among these maternally deposited mRNAs and are robustly translated following fertilization. In Drosophila, the mRNA encoding Zelda, the major activator of the zygotic genome, is not translated until 1 h after fertilization. Here we demonstrate that zelda translation is repressed in the early embryo by the TRIM-NHL protein Brain tumor (BRAT). BRAT also regulates Zelda levels in the larval neuroblast lineage. In the embryo, BRAT-mediated translational repression is regulated by the Pan Gu kinase, which is triggered by egg activation. The Pan Gu kinase phosphorylates translational regulators, suggesting that Pan Gu kinase activity alleviates translational repression of zelda by BRAT and coupling translation of zelda with that of other regulators of early embryonic development. Using the premature translation of zelda in embryos lacking BRAT activity, we showed that early translation of a zygotic genome activator is not sufficient to drive precocious gene expression. Instead, Zelda-target genes showed increased expression at the time they are normally activated. We propose that transition through early development requires the integration of multiple processes, including the slowing of the nuclear division cycle and activation of the zygotic genome. These processes are coordinately controlled by Pan Gu kinase-mediated regulation of translation.
The PAICE suite reveals circadian posttranscriptional timing of noncoding RNAs and spliceosome components in Mus musculus macrophages
Authors: Buel Sharleen M , Debopadhaya Shayom , De los Santos Hannah , Edwards Kaelyn M , David Alexandra M , Dao Uyen H , Bennett Kristin P , Hurley Jennifer M
Published: July 2022

Circadian rhythms broadly regulate physiological functions by tuning oscillations in the levels of mRNAs and proteins to the 24-h day/night cycle. Globally assessing which mRNAs and proteins are timed by the clock necessitates accurate recognition of oscillations in RNA and protein data, particularly in large omics data sets. Tools that employ fixed-amplitude models have previously been used to positive effect. However, the recognition of amplitude change in circadian oscillations required a new generation of analytical software to enhance the identification of these oscillations. To address this gap, we created the Pipeline for Amplitude Integration of Circadian Exploration suite. Here, we demonstrate the Pipeline for Amplitude Integration of Circadian Exploration suite’s increased utility to detect circadian trends through the joint modeling of the Mus musculus macrophage transcriptome and proteome. Our enhanced detection confirmed extensive circadian posttranscriptional regulation in macrophages but highlighted that some of the reported discrepancy between mRNA and protein oscillations was due to noise in data. We further applied the Pipeline for Amplitude Integration of Circadian Exploration suite to investigate the circadian timing of noncoding RNAs, documenting extensive circadian timing of long noncoding RNAs and small nuclear RNAs, which control the recognition of mRNA in the spliceosome complex. By tracking oscillating spliceosome complex proteins using the PAICE suite, we noted that the clock broadly regulates the spliceosome, particularly the major spliceosome complex. As most of the above-noted rhythms had damped amplitude changes in their oscillations, this work highlights the importance of the PAICE suite in the thorough enumeration of oscillations in omics-scale datasets.
The genome of Dioscorea zingiberensis sheds light on the biosynthesis, origin and evolution of the medicinally important diosgenin saponins
Authors: Li Yi , Tan Chao , Li Zihao , Guo Jingzhe , Li Song , Chen Xin , Wang Chen , Dai Xiaokang , Yang Huan , Song Wei , Hou Lixiu , Xu Jiali , Tong Ziyu , Xu Anran , Yuan Xincheng , Wang Weipeng , Yang Qingyong , Chen Lingling , Sun Zongyi , Wang Kai , Pan Bo , Chen Jianghua , Bao Yinghua , Liu Faguang , Qi Xiaoquan , Gang David R , Wen Jun , Li Jiaru
Published: July 2022

Diosgenin saponins isolated from Dioscorea species such as D. zingiberensis exhibit a broad spectrum of pharmacological activities. Diosgenin, the aglycone of diosgenin saponins, is an important starting material for the production of steroidal drugs. However, how plants produce diosgenin saponins and the origin and evolution of the diosgenin saponin biosynthetic pathway remain a mystery. Here we report a high-quality, 629-Mb genome of D. zingiberensis anchored on 10 chromosomes with 30 322 protein-coding genes. We reveal that diosgenin is synthesized in leaves (‘source’), then converted into diosgenin saponins, and finally transported to rhizomes (‘sink’) for storage in plants. By evaluating the distribution and evolutionary patterns of diosgenin saponins in Dioscorea species, we find that diosgenin saponin-containing may be an ancestral trait in Dioscorea and is selectively retained. The results of comparative genomic analysis indicate that tandem duplication coupled with a whole-genome duplication event provided key evolutionary resources for the diosgenin saponin biosynthetic pathway in the D. zingiberensis genome. Furthermore, comparative transcriptome and metabolite analysis among 13 Dioscorea species suggests that specific gene expression patterns of pathway genes promote the differential evolution of the diosgenin saponin biosynthetic pathway in Dioscorea species. Our study provides important insights and valuable resources for further understanding the biosynthesis, evolution, and utilization of plant specialized metabolites such as diosgenin saponins.
The compressive study of energy security prospects in India through Solar Power
Authors: Prakash Om , Ahmad Asim , Kumar Anil , Chatterjee Rajeshwari , Sharma Shubham , Alayi Reza , Monfared Hossein
Published: July 2022

Energy security depends upon the supply and demand of electricity. Power supply volatility is a challenge in India power sector. India is mainly dependent on coal, which is not a viable long-term option. Hence the future of energy supply in India is renewable energy, and solar energy is the most prominent and reliable source of renewable energy. The solar power plant is classified as a low, medium, and high temperature based solar power plant. The low- temperature solar power plant, the collectors, are unglazed plates. In contract, medium-temperature collectors are flat plates, and high-temperature collectors concentrate sunlight using mirrors and lenses, achieving temperature and pressure up to 3000 °C and 20 bar. This article discusses the existing total energy scenario, current solar energy developments, supportive solar energy policies, and solar energy prospects. The tactics, availability, perspectives, potential, and successes of solar energy in India are discussed in this study. The authors compared the barriers to solar energy acceptance and examined the topic of social dissimilarity from a broad theoretical perspective. The study offers policymakers at the federal and state levels, as well as investors, a perspective on issues that may lead to investments in this area. The study states that solar energy could meet above 50% of electricity sector demand in India in 2040. This study determined that solar energy incidence in India is around 5000 trillion KWh (kilowatt-hours) each year. The solar energy accessible in a single year surpasses the energy output from the petroleum derivative. The average energy from the solar power plant is 0.30 kWh per m2 equal to the 1400–1800 peak rated capacity. India has many solar power facilities, making it one of the top producers of renewable energy power.
Drop-in assessment of plug-in R404A refrigeration equipment using low-global warming potential mixtures
Authors: Devecioğlu Atilla G , Oruç Vedat
Published: July 2022

This study analyses the performance of plug-in refrigeration equipment, which has the condenser on the system and delivers heat to the indoor medium, using mixtures with low-global warming potential (GWP). The theoretical drop-in replacement of R404A that is widely used in the plug-in refrigeration equipment, is investigated utilizing refrigerants with GWP<150 such as R454C, R455A, R457A and R465A. In the study, four cases of evaporation temperatures (-25°C, -15°C, -5°C and 0°C) and two cases of condenser temperatures (30°C and 40°C) were considered for freezer and chiller modes of the equipment. According to the obtained results, the power consumption of R404A is determined to be higher than other refrigerants for both studied condenser temperatures. The mass flow rate circulating in the system decreases using R404A’s alternatives. Furthermore, the highest COP is reached with R457A which has a greater COP than R404A about by 8.5%. The compressor discharge pressures of alternative refrigerants are smaller compared to R404A as well. The reduction in CO2 emission is also presented in terms of total equivalent warming impact (TEWI) computations. The conducted analysis points out that it will be possible to use directly the investigated mixtures (especially R457A and R455A are most promising ones) in plug-in refrigeration devices as alternatives to R404A without performing any constructional modification in the system.
Prototype Pathogen Approach for Vaccine and Monoclonal Antibody Development: A Critical Component of the NIAID Plan for Pandemic Preparedness
Authors: Cassetti M Cristina , Pierson Theodore C , Patterson L Jean , Bok Karin , DeRocco Amanda J , Deschamps Anne M , Graham Barney S , Erbelding Emily J , Fauci Anthony S
Published: July 2022

Severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1) emerged 20 years ago, presaging a series of subsequent infectious disease epidemics of international concern. The recent emergence of SARS-CoV-2 has underscored the importance of targeted preparedness research to enable rapid countermeasure development during a crisis. In December 2021 the National Institute of Allergy and Infectious Diseases (NIAID), building upon the successful strategies developed during the SARS-CoV-2 response and to prepare for future pandemics, published a pandemic preparedness plan that outlined a research strategy focused on priority pathogens, technology platforms, and prototype pathogens. To accelerate the discovery, development, and evaluation of medical countermeasures against new or previously unknown pathogens of pandemic potential, we present here a strategy of research directed at select prototype pathogens. In this manner, leveraging a prototype pathogen approach may serve as a powerful cornerstone in biomedical research preparedness to protect public health from newly emerging and reemerging infectious diseases.
Sensitivity of Reverse Transcription Polymerase Chain Reaction Tests for Severe Acute Respiratory Syndrome Coronavirus 2 Through Time
Authors: Binny Rachelle N , Priest Patricia , French Nigel P , Parry Matthew , Lustig Audrey , Hendy Shaun C , Maclaren Oliver J , Ridings Kannan M , Steyn Nicholas , Vattiato Giorgia , Plank Michael J
Published: July 2022

Background

Reverse transcription polymerase chain reaction (RT-PCR) tests are the gold standard for detecting recent infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Reverse transcription PCR sensitivity varies over the course of an individual’s infection, related to changes in viral load. Differences in testing methods, and individual-level variables such as age, may also affect sensitivity.

Methods

Using data from New Zealand, we estimate the time-varying sensitivity of SARS-CoV-2 RT-PCR under varying temporal, biological, and demographic factors.

Results

Sensitivity peaks 4–5 days postinfection at 92.7% (91.4%–94.0%) and remains over 88% between 5 and 14 days postinfection. After the peak, sensitivity declined more rapidly in vaccinated cases compared with unvaccinated, females compared with males, those aged under 40 compared with over 40s, and Pacific peoples compared with other ethnicities.

Conclusions

Reverse transcription PCR remains a sensitive technique and has been an effective tool in New Zealand’s border and postborder measures to control coronavirus disease 2019. Our results inform model parameters and decisions concerning routine testing frequency.
Spending Windfall (“Found”) Time on Hedonic versus Utilitarian Activities
Authors: Chung Jaeyeon (Jae) , Lee Leonard , Lehmann Donald R , Tsai Claire I
Published: July 2022

Consumers often gain extra free time unexpectedly. Given the increasing time pressure that consumers experience in their daily lives, it is important to understand how they spend windfall (or unexpected) free time, which we term found time. In a series of five laboratory experiments and naturalistic field studies, we found that consumers spend more of their free time on hedonic activities than on utilitarian activities when they gain the time unexpectedly (i.e., found time), but not when they know about the free time in advance. This pattern occurs consistently regardless of whether consumers gain the time from canceled work-related or leisure activities. In addition, our studies uncovered perceived busyness as a ubiquitous yet unexplored moderator for the windfall gain literature: the inclination to allocate found time to hedonic consumption decreases when consumers perceive themselves to be busy at the moment. We discuss several potential accounts for the effect of unexpectedness on time expenditure, including a perceived fit between the nature of found time (a fun windfall gain) and hedonic consumption, need for justification, and planning.
Trace metals at the frontline of pathogen defence responses in non-hyperaccumulating plants
Authors: Morina Filis , Küpper Hendrik
Published: July 2022

Metal hyperaccumulation is an exclusive evolutionary trait contributing to efficient plant defence against biotic stress. The defence can be based on direct metal toxicity or the joint effects of accumulated metal and organic compounds, the latter being based on integrated signalling networks. While the role of metals in biotic stress defence of hyperaccumulators has been intensively studied, their role in the pathogen immunity of non-accumulator plants is far less understood. New findings show that in metal non-hyperaccumulating plants, localized hot spots of zinc, manganese, and iron increase plant immunity, while manipulation of nutrient availability may be used for priming against subsequent pathogen attack. Recent findings on the role of metals in plant–pathogen interactions are discussed considering the narrow line between deficiency and toxicity, host–pathogen nutrient competition and synergistic effects of simultaneous metal and biotic stress. We discuss the suitability of the direct-defence and joint-effects hypotheses in non-hyperaccumulating plants, and the involvement of metals as active centres of immunity-related enzymes. We also consider future challenges in revealing the mechanisms underlying metal-mediated plant immunity.
Similarity solutions for a magnetized supercritical accretion disc around a rotating object
Authors: Habibi Fahimeh
Published: July 2022

The effect of toroidal magnetic fields on a supercritical accretion disc (slim disc) around a rotating object is examined. In this research, it is supposed that angular momentum transport is due to viscous turbulence and the α-prescription is used for the kinematic coefficient of viscosity. Moreover, the general relativistic effects are neglected. The degree of advection that demonstrates the fraction of energy that accretes by matter on to the central object is considered by f parameter. For the steady-state structure of such accretion flows, a set of self-similar solution is presented. Our solutions will include two important non-dimensional parameters β and a. β is the ratio of the magnetic pressure to the gas pressure, the so-called friction of magnetic pressure, which shows the magnetic field strength. The ratio of the angular velocities of the central body and the accretion flow is indicated by the rotating parameter a. The possible combined effects of magnetic field, spin of central object, and degree of advection are investigated. We also show the effect of rotating parameter a on the physical quantities of disc is different for co-rotating and counter-rotating flows. Moreover, by increasing the degree of advection and strength of magnetic field, the behaviour of the radial and angular velocities becomes reversed with respect to a. The model implies that the surface temperature, thickness, and luminosity of disc strongly depend on rotation parameter and strength of magnetic field.
Adjunctive Recombinant Human Plasma Gelsolin for Severe Coronavirus Disease 2019 Pneumonia
Authors: DiNubile Mark J , Parra Sandra , Salomó Antoni Castro , Levinson Susan L
Published: July 2022

Background

Excessive inflammation contributes to the morbidity and mortality of severe coronavirus disease 2019 (COVID-19) pneumonia. Recombinant human plasma gelsolin (rhu-pGSN) improves disease outcomes in diverse experimental models of infectious and noninfectious inflammation.

Methods

In a blinded, randomized study, 61 subjects with documented COVID-19 pneumonia having a World Health Organization (WHO) Severity Score of 4 to 6 and evidence of a hyperinflammatory state were treated with standard care and either adjunctive rhu-pGSN 12 mg/kg or an equal volume of saline placebo given intravenously at entry, 12 hours, and 36 hours. The prespecified coprimary outcomes were survival without major respiratory, hemodynamic, or renal support on Day 14 and the incidence of serious adverse events (SAEs) during the 90-day study period.

Results

All subjects receiving ≥1 dose of study drug were analyzed. Fifty-four of 61 subjects (88.5%) were WHO severity level 4 at entry. The proportions of subjects alive without support on Day 14 were 25 of 30 rhu-pGSN recipients (83.3%) and 27 of 31 placebo recipients (87.1%). Over the duration of the study, WHO Severity Scores improved similarly in both treatment groups. No statistically significant differences were observed between treatment groups at any time point examined. Two subjects died in each group. Numerically fewer subjects in the rhu-pGSN group had SAEs (5 subjects; 16.7%) or ≥ Grade 3 adverse events (5 subjects; 16.7%) than in the placebo group (8 subjects [25.8%] and 9 subjects [29.0%], respectively), mostly involving the lungs. Three rhu-pGSN recipients (10.0%) were intubated compared to 6 placebo recipients (19.4%).

Conclusions

Overall, subjects in this study did well irrespective of treatment arm. When added to dexamethasone and remdesivir, no definitive benefit was demonstrated for rhu-pGSN relative to placebo. Safety signals were not identified after the administration of 3 doses of 12 mg/kg rhu-pGSN over 36 hours. The frequencies of SAEs and intubation were numerically fewer in the rhu-pGSN group compared with placebo.
Experimental Investigation of Trachydacite Magma Storage Prior to the 1257 Eruption of Mt Samalas
Authors: Voigt Annika , Cassidy Michael , Castro Jonathan M , Pyle David M , Mather Tamsin A , Helo Christoph , Abdurrachman Mirzam , Kurniawan Idham Andri
Published: July 2022

The caldera-forming Samalas eruption of the Samalas–Rinjani volcanic complex on Lombok, Indonesia, in 1257 CE ranks as one of the most explosive and sulphur-rich eruptions of the past thousand years. Along with other significant events (e.g. Tambora 1815, El Chichón 1982), Samalas 1257 forms a class of powerful eruptions of magmas of intermediate alkaline composition, which remain relatively understudied. This study aims to better understand the pre-eruptive magma reservoir conditions and volatile storage capacities of intermediate alkaline systems, using the 1257 Samalas eruption as a case study. Systematic partial-equilibrium experiments were run to constrain potential magma reservoir conditions (25–200 MPa and 850–1000°C) by examining changes in the chemistry and textures of experimental glass and mineral products. Natural trachydacite pumice from the 1257 Samalas eruption was used as the experimental charge for a series of water-saturated experiments run in cold-seal pressure vessels, buffered at an oxygen fugacity ƒO2 of NNO/NNO + 1 log units. Assessing the match between natural and experimental glass compositions and mineral phases and textures reveals that pre-eruptive magma reservoir conditions were between 875°C and 930°C and ca. 100–150 MPa (4.5 ± 1km depth). Breakdown of experimental amphibole at pressures below 75 MPa, and plagioclase instability at 950°C/100 MPa, provide the strongest constraints on phase stabilities that are consistent with the magma storage region. In the observed temperature and pressure range, the natural compositions of plagioclase, amphibole and orthopyroxene are replicated in experimentally precipitated rims. Clinopyroxene and high anorthite plagioclase (An>64) in the natural mineral assemblage could not be replicated in the experiments, implying that these compositions most likely did not grow in the shallow pre-eruptive reservoir of Mt Samalas. The pre-eruptive storage depths of trachydacite magma are significantly shallower than the postulated magma storage at the currently active basaltic-andesite magmatic system at Rinjani, and thus monitoring magma storage depth may be a useful indicator of changing magma composition in the future. Our findings show that highly explosive VEI 7 eruptions of intermediate alkaline magma can be fed from a relatively limited range of storage pressures (100–150 MPa), suggesting accumulation of magma in one place, rather than the rapid extraction of magma from a vertically extensive, transcrustal magma system.
ADP-ribosylation factor D1 modulates Golgi morphology, cell plate formation, and plant growth in Arabidopsis
Authors: Niu Fangfang , Ji Changyang , Liang Zizhen , Guo Rongfang , Chen Yixuan , Zeng Yonglun , Jiang Liwen
Published: July 2022

ADP-ribosylation factor (ARF) family proteins, one type of small guanine-nucleotide-binding (G) proteins, play a central role in regulating vesicular traffic and organelle structures in eukaryotes. The Arabidopsis (Arabidopsis thaliana) genome contains more than 21 ARF proteins, but relatively little is known about the functional heterogeneity of ARF homologs in plants. Here, we characterized the function of a unique ARF protein, ARFD1B, in Arabidopsis. ARFD1B exhibited both cytosol and punctate localization patterns, colocalizing with a Golgi marker in protoplasts and transgenic plants. Distinct from other ARF1 homologs, overexpression of a dominant-negative mutant form of ARFD1B did not alter the localization of the Golgi marker mannosidase I (ManI)-RFP in Arabidopsis cells. Interestingly, the ARFD1 artificial microRNA knockdown mutant arfd1 displayed a deleterious growth phenotype, while this phenotype was restored in complemented plants. Further, confocal imaging and transmission electron microscopy analyses of the arfd1 mutant revealed defective cell plate formation and abnormal Golgi morphology. Pull-down and liquid chromatography–tandem mass spectrometry analyses identified Coat Protein I (COPI) components as interacting partners of ARFD1B, and subsequent bimolecular fluorescence complementation, yeast (Saccharomyces cerevisiae) two-hybrid, and co-immunoprecipitation assays further confirmed these interactions. These results demonstrate that ARFD1 is required for cell plate formation, maintenance of Golgi morphology, and plant growth in Arabidopsis.
FLT3 functional low-frequency variant rs76428106-C is associated with susceptibility to systemic sclerosis
Authors: Martínez-López Javier , Kerick Martin , Ortiz-Fernández Lourdes , Acosta-Herrera Marialbert , Márquez Ana , Martín Javier
Published: July 2022

Objectives

rs76428106-C, a low frequency polymorphism that affects the splicing of the FLT3 gene, has recently been associated with several seropositive autoimmune diseases. Here, we aimed to evaluate the potential implication of rs76428106-C in the susceptibility to systemic sclerosis (SSc).

Methods

We analysed a total of 26 598 European ancestry individuals, 9063 SSc and 17 535 healthy controls, to test the association between FLT3 rs76428106-C and SSc and its different subphenotypes. Genotype data of rs76428106 were obtained by imputation of already available genome-wide association study data and analysed by logistic regression analysis.

Results

In accordance with that observed in other autoimmune disorders, the FLT3 rs76428106-C allele was significantly increased [P-value = 2.03 × 10−3, odds ratio (OR) = 1.34] in SSc patients compared with healthy controls. A similar risk effect was found when the main SSc clinical and serological subgroups were compared with controls. When comparing SSc patients with and without digital ulcers (DU), the rs76428106-C frequency was significantly increased in DU-positive SSc patients in comparison with DU-negative patients (P-value = 0.036, OR = 2.16).

Conclusion

This study is the first to report an association between rs76428176-C and SSc. Our results support the role of FLT3 as a relevant gene in seropositive immune-mediated diseases and a potential biomarker for SSc microangiopathy.
Homeostatic response to sleep deprivation and circadian rhythmicity are intact in older adults with insomnia
Authors: Tracy Eunjin Lee , Zhang Jun , Wilckens Kristine , Krafty Robert T , Hasler Brant P , Hall Martica H , Buysse Daniel J
Published: July 2022

Study Objectives

We examined whether homeostatic sleep drive and circadian rhythmicity differ in older adults with insomnia (OAI) compared to older good sleepers (GS).

Methods

OAI (n = 37) and GS (n = 30) participated in a 60-h in-lab study with sleep deprivation and constant routine paradigms. Homeostatic sleep drive was assessed by examining the effect of sleep deprivation on delta EEG power and theta EEG power, and repeated sleep latency tests. Circadian rhythm was assessed with salivary melatonin (phase and amplitude), core body temperature (phase, amplitude, and mesor), and sleep latency during a constant routine paradigm. Mixed models were used to assess interactions of group (OAS vs GS) with homeostatic sleep and circadian effects.

Results

Compared to GS, OAI showed a greater linear increase in waking theta power during sleep deprivation, but the two groups did not show differential responses to sleep deprivation in delta EEG, or in repeated sleep latency tests. The two groups did not differ in circadian phase or amplitude of melatonin or core body temperature rhythms. OAI had a significantly elevated core body temperature mesor compared to GS.

Conclusions

Homeostatic response to sleep deprivation was intact in OAI compared to GS; theta EEG power suggested a greater homeostatic response in OAI. Circadian rhythm amplitude and phase were similar in OAI compared to GS. Elevated body temperature mesor in OAI may indicate elevated physiological arousal. These findings suggest that effective treatments for insomnia in older adults may leverage intact sleep and circadian regulatory mechanisms, rather than repair defective sleep and circadian regulation.
Sleep spindle and slow wave activity in Parkinson disease with excessive daytime sleepiness
Authors: Schreiner Simon J , Werth Esther , Ballmer Leonie , Valko Philipp O , Schubert Kai M , Imbach Lukas L , Baumann Christian R , Maric Angelina , Baumann-Vogel Heide
Published: July 2022

Study Objectives

Excessive daytime sleepiness (EDS) is a common and devastating symptom in Parkinson disease (PD), but surprisingly most studies showed that EDS is independent from nocturnal sleep disturbance measured with polysomnography. Quantitative electroencephalography (EEG) may reveal additional insights by measuring the EEG hallmarks of non-rapid eye movement (NREM) sleep, namely slow waves and spindles. Here, we tested the hypothesis that EDS in PD is associated with nocturnal sleep disturbance revealed by quantitative NREM sleep EEG markers.

Methods

Patients with PD (n = 130) underwent polysomnography followed by spectral analysis to calculate spindle frequency activity, slow-wave activity (SWA), and overnight SWA decline, which reflects the dissipation of homeostatic sleep pressure. We used the Epworth Sleepiness Scale (ESS) to assess subjective daytime sleepiness and define EDS (ESS > 10). All examinations were part of an evaluation for deep brain stimulation.

Results

Patients with EDS (n = 46) showed reduced overnight decline of SWA (p = 0.036) and reduced spindle frequency activity (p = 0.032) compared with patients without EDS. Likewise, more severe daytime sleepiness was associated with reduced SWA decline (ß= −0.24 p = 0.008) and reduced spindle frequency activity (ß= −0.42, p < 0.001) across all patients. Reduced SWA decline, but not daytime sleepiness, was associated with poor sleep quality and continuity at polysomnography.

Conclusions

Our data suggest that daytime sleepiness in PD patients is associated with sleep disturbance revealed by quantitative EEG, namely reduced overnight SWA decline and reduced spindle frequency activity. These findings could indicate that poor sleep quality, with incomplete dissipation of homeostatic sleep pressure, may contribute to EDS in PD.
A small slug from a tropical greenhouse reveals a new rathouisiid lineage with triaulic tritrematic genitalia (Gastropoda: Systellommatophora)
Authors: Manganelli Giuseppe , Lesicki Andrzej , Benocci Andrea , Barbato Debora , Miserocchi Danio , Pieńkowska Joanna R , Giusti Folco
Published: July 2022

A small slug found in the tropical greenhouse of the Science Museum (MUSE) of Trento (Italy) turned out to be a species of the little-known systellommatophoran family Rathouisiidae. We undertook detailed comparative anatomical and molecular studies using specimens of the MUSE slug, Rathouisia sinensis, and sequences of other systellommatophoran species deposited in GenBank to conduct a systematic and phylogenetic assessment. Analysis of the genitalia of the MUSE slug and R. sinensis revealed an unusual triaulic tritrematic structure: two separate female ducts – one for egg release (oviduct), the other for intake of allosperm (vagina) – and a separate male duct for autosperm release. Analysis of the nucleotide sequences of several mitochondrial (COI, 16S rDNA) and nuclear (18S rDNA, ITS2 flanked by 5.8S and 28S rDNA fragments, H3) gene fragments supported assignation of the MUSE slug to Rathouisiidae, but also its distinction from the other rathouisiid genera Atopos, Granulilimax, Rathouisia and an undescribed genus from the Ryukyu Islands (Japan). Therefore, we decided to describe the MUSE slug as a new species in a new genus: Barkeriella museensis gen. et sp. nov. The species is certainly an alien introduced into the tropical greenhouse of MUSE, but its origin is unknown and calls for further investigation.
A New Technique for Breast Pocket Adjustment: Argon Beam Thermal Capsulorrhaphy
Authors: Awaida Cyril J. , Paek Laurence , Danino Michel Alain
Published: July 2022

Implant malposition remains one of the main complications of aesthetic breast augmentation and alloplastic breast reconstruction with expanders and implants. Many capsulorrhaphy techniques have been described to adjust the breast pocket and correct the malposition. In this study, we tested the efficacy of the argon beam coagulator (ABC) for lateral capsulorrhaphy on breast reconstruction patients at the time of expander replacement with a permanent implant. We also experimentally compared the effects of the ABC and the standard electrocautery on fragments of healthy breast capsule. We noted a 69.5% capsule shrinkage with the ABC versus 46.8% with the standard electrocautery. We concluded that breast capsulorrhaphy using the ABC is a safe and efficient technique for the correction of breast implant malposition in both reconstructive and aesthetic breast surgery.
AGE-RELATED RETENTIONAL AVASCULAR PIGMENT EPITHELIAL DETACHMENT VIEWED WITH INDOCYANINE GREEN ANGIOGRAPHY
Authors: Su Yongyue , Zhang Xiongze , Chen Ling , Li Miaoling , Gan Yuhong , Wen Feng
Published: July 2022

Purpose:

Age-related scattered hypofluorescent spots on late-phase indocyanine green angiography (ASHS-LIA) might represent hydrophobic neutral lipid deposits in the Bruch membrane. This study aimed to report retentional avascular pigment epithelial detachment (PED) associated with ASHS-LIA.

Methods:

Patients aged ≥50 years who presented a single avascular serous PED without soft drusen or any other retinal or choroidal diseases were retrospectively included. Pigment epithelial detachment was classified as retentional, effusional, or mixed PED based on indocyanine green angiography. Multimodal images were qualitatively and quantitatively evaluated.

Results:

This study included 74 eyes of 57 patients. Retentional PED, effusional PED, and mixed PED accounted for 91.9%, 4.1%, and 4.1%, respectively. All PEDs were located in the macular region. Seventeen (29.8%) included patients had bilateral PEDs and all were retentional PEDs with a high level of bilateral consistency in the characteristics of PED and ASHS-LIA. All retentional PEDs were within the bounds of ASHS-LIA. The area of retentional PED increased with the ASHS-LIA grade (P = 0.030).

Conclusion:

Most age-related avascular serous PEDs are retentional PEDs. The location and area of retentional PEDs are consistent with the distribution of ASHS-LIA. These findings suggest that the hydrophobic neutral lipid deposits in the Bruch membrane might be involved in the pathogenesis and be a therapeutic target in age-related retentional avascular PED.
INTRAVITREAL ANTIVASCULAR ENDOTHELIAL GROWTH FACTOR FOR THE TREATMENT OF CHOROIDAL NEOVASCULARIZATION SECONDARY TO OCULAR HISTOPLASMOSIS
Authors: Dudenhoefer Nicholas E. , Noble Carl W. , Petersen Michael R. , Foster Robert E. , Riemann Christopher D. , Sisk Robert A. , Hutchins Robert K. , Miller Daniel M.
Published: July 2022

Purpose:

To assess the long-term efficacy of intravitreal antivascular endothelial growth factor injections (IVI), alone or in combination with verteporfin photodynamic therapy (IVI/PDT), for management of choroidal neovascularization secondary to presumed ocular histoplasmosis syndrome (POHS).

Methods:

Retrospective, comparative, interventional case series analyzing 82 eyes in 74 patients treated with either IVI or IVI/PDT for presumed ocular histoplasmosis syndrome choroidal neovascularization from January 2006 to January 2021.

Results:

The average logarithm of the minimum angle of resolution VA in year 5 was 0.40 (20/50) and 0.52 (20/67) for IVI versus IVI/PDT groups, respectively (P = 0.33), and in year 10 was 0.53 (20/58) and 0.64 (20/86), respectively (P = 0.50). The average number of annual injections over the first 5 years of follow-up was 3.3 versus 1.7 for IVI versus IVI/PDT groups, respectively (P < 0.001), and over 10 years was 3.3 versus 1.6, respectively (P < 0.001). Treatment-free interval of 5 years was reached by 39% versus 60% in IVI versus IVI/PDT groups, respectively (P = 0.95).

Conclusion:

Our study found both IVI and IVI/PDT to be effective in long-term management of presumed ocular histoplasmosis syndrome choroidal neovascularization, with a fewer number of annual injections and longer treatment-free interval in the combination group. However, given the limitations of a retrospective study, a prospective randomized study is necessary to determine whether the addition of PDT significantly decreases treatment burden.
Prehospital Tranexamic Acid in Major Pediatric Trauma Within a Physician-Led Emergency Medical Services System: A Multicenter Retrospective Study
Authors: Gossiome Amaury , Claustre Clément , Fraticelli Laurie , Jacquet Landry , Bouchut Jean-Christophe , Javouhey Etienne , Courtil-Teyssedre Sonia , Taverna Xavier-Jean , David Jean-Stéphane , Mercier Eric , Tazarourte Karim , El Khoury Carlos , Benhamed Axel
Published: July 2022

Objectives:

Describe prehospital tranexamic acid (TXA) use and appropriateness within a major trauma pediatric population, and identify the factors associated with its use.

Design:

Multicenter, retrospective study, 2014–2020.

Setting:

Data were extracted from a multicenter French trauma registry including nine trauma centers within a physician-led prehospital emergency medical services (EMS) system.

Patients:

Patients less than 18 years old were included. Those who did not receive prehospital intervention by a mobile medical team and those with missing data on TXA administration were excluded.

Interventions:

None.

Measurements and Main Results:

Nine-hundred thirty-four patients (median [interquartile range] age: 14 yr [9–16 yr]) were included, and 68.6% n = 639) were male. Most patients were involved in a road collision (70.2%, n = 656) and suffered a blunt trauma (96.5%; n = 900). Patients receiving TXA (36.6%; n = 342) were older (15 [13–17] vs 12 yr [6–16 yr]) compared with those who did not. Patient severity was higher in the TXA group (Injury Severity Score 14 [9–25] vs 6 [2–13]; p < 0.001). The median dosage was 16 mg/kg (13–19 mg/kg). TXA administration was found in 51.8% cases (n = 256) among patients with criteria for appropriate use. Conversely, 32.4% of patients (n = 11) with an isolated severe traumatic brain injury (TBI) also received TXA. Age (odds ratio [OR], 1.2; 95% CI, 1.1–1.2), A and B prehospital severity grade (OR, 7.1; 95% CI, 4.1–12.3 and OR, 4.5; 95% CI, 2.9–6.9 respectively), and year of inclusion (OR, 1.2; 95% CI, 1.1–1.3) were associated with prehospital TXA administration.

Conclusions:

In our physician-led prehospital EMS system, TXA is used in a third of severely injured children despite the lack of high-level of evidence. Only half of the population with greater than or equal to one criteria for appropriate TXA use received it. Conversely, TXA was administered in a third of isolated severe TBI. Further research is warranted to clarify TXA indications and to evaluate its impact on mortality and its safety profile to oversee its prescription.
Moral Distress in the Italian PICUs: The Role of Individual and Organizational Factors*
Authors: Lamiani Giulia , Barlascini Luca , Borghi Lidia , Giannini Alberto ,   on behalf of the Open Doors in INtensive care (ODIN) Study Group 2
Published: July 2022

Objectives:

To assess the interplay between individual and organizational factors on moral distress in PICUs.

Design:

Cross-sectional, article-based survey administered between June and August 2017.

Setting:

Twenty-three Italian PICUs.

Subjects:

Of 874 eligible clinicians, 635 responded to the survey (75% response rate), and 612 correctly completed the survey. Clinicians were 74% female; 66% nurses and 34% physicians; and 51% had between 6 and 20 years of experience from graduation.

Intervention:

None.

MEASUREMENTS AND MAIN RESULTS:

Clinicians completed the “Italian Moral Distress Scale-Revised,” the “Connor-Davidson Resilience Scale,” and a sociodemographic questionnaire inquiring about sex, profession, years of experience, and spiritual/religious belief. PICU organizational characteristics were also collected (e.g., number of admissions, mortality rate, nurse-patient ratio, presence of parents in PICU, presence of parents during physical examination). To identify individual predictors of moral distress, we used stepwise linear regression. To determine the impact of organizational factors on moral distress, we used analysis of covariance, controlling for those individual factors that emerged as significant in the regression model. Moral distress was associated with being a nurse (B = –0.094; p < 0.05) and not having spiritual/religious belief (B = 0.130; p < 0.01), but not with resilience. Clinicians working in PICUs with a medium number of admissions per year (between 264 and 468), a lower nurse:patient ratio (1:3), and where parents’ presence during physical examination were never allowed experienced higher moral distress even controlling for profession and spiritual/religious belief.

Conclusions:

Organizational factors (medium number of admissions, lower nurse:patient ratio, and parental exclusion from physical examination) played a stronger role than individual factors in increasing moral distress. To decrease moral distress, attention should be paid also to organizational aspects.
Determinants of antibody response to severe acute respiratory syndrome coronavirus 2 mRNA vaccines in people with HIV
Authors: Chammartin Frédérique , Kusejko Katharina , Pasin Chloé , Trkola Alexandra , Briel Matthias , Amico Patrizia , Stoekle Marcel P. , Eichenberger Anna L. , Hasse Barbara , Braun Dominique L. , Schuurmans Macé M. , Müller Thomas F. , Tamm Michael , Mueller Nicolas J. , Rauch Andri , Koller Michael T. , Günthard Huldrych F. , Bucher Heiner C. , Speich Benjamin , Abela Irene A.
Published: July 2022

We identified determinants of SARS-CoV-2 mRNA vaccine antibody response in people with HIV (PWH). Antibody response was higher among PWH less than 60 years, with CD4+ cell count superior to 350 cells/μl and vaccinated with mRNA-1273 by Moderna compared with BNT162b2 by Pfizer-BioNTech. Preinfection with SARS-CoV-2 boosted the antibody response and smokers had an overall lower antibody response. Elderly PWH and those with low CD4+ cell count should be prioritized for booster vaccinations.