bluuebunny/crossref_metadata_2025 huggingface dataset:
GC-MS Analysis and Biomedical Therapy of Oil from n-Hexane Fraction of Scutellaria edelbergii Rech. f.: In Vitro, In Vivo, and In Silico Approach
Authors: Shah Muddaser , Murad Waheed , Ur Rehman Najeeb , Mubin Sidra , Al-Sabahi Jamal Nasser , Ahmad Manzoor , Zahoor Muhammad , Ullah Obaid , Waqas Muhammad , Ullah Saeed , Kamal Zul , Almeer Rafa , Bungau Simona G. , Al-Harrasi Ahmed
Published: December 2021

The current study aimed to explore the crude oils obtained from the n-hexane fraction of Scutellaria edelbergii and further analyzed, for the first time, for their chemical composition, in vitro antibacterial, antifungal, antioxidant, antidiabetic, and in vivo anti-inflammatory, and analgesic activities. For the phytochemical composition, the oils proceeded to gas chromatography-mass spectrometry (GC-MS) analysis and from the resultant chromatogram, 42 bioactive constituents were identified. Among them, the major components were linoleic acid ethyl ester (19.67%) followed by ethyl oleate (18.45%), linolenic acid methyl ester (11.67%), and palmitic acid ethyl ester (11.01%). Tetrazolium 96-well plate MTT assay and agar-well diffusion methods were used to evaluate the isolated oil for its minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC), half-maximal inhibitory concentrations (IC50), and zone of inhibitions that could determine the potential antimicrobial efficacy’s. Substantial antibacterial activities were observed against the clinical isolates comprising of three Gram-negative bacteria, viz., Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, and one Gram-positive bacterial strain, Enterococcus faecalis. The oils were also effective against Candida albicans and Fusarium oxysporum when evaluated for their antifungal potential. Moreover, significant antioxidant potential with IC50 values of 136.4 and 161.5 µg/mL for extracted oil was evaluated through DPPH (1,1-Diphenyl-2-picryl-hydrazyl) and ABTS assays compared with standard ascorbic acid where the IC50 values were 44.49 and 67.78 µg/mL, respectively, against the tested free radicals. The oils was also potent, inhibiting the α-glucosidase (IC50 5.45 ± 0.42 µg/mL) enzyme compared to the standard. Anti-glucosidase potential was visualized through molecular docking simulations where ten compounds of the oil were found to be the leading inhibitors of the selected enzyme based on interactions, binding energy, and binding affinity. The oil was found to be an effective anti-inflammatory (61%) agent compared with diclofenac sodium (70.92%) via the carrageenan-induced assay. An appreciable (48.28%) analgesic activity in correlation with the standard aspirin was observed through the acetic acid-induced writhing bioassay. The oil from the n-hexane fraction of S. edelbergii contained valuable bioactive constituents that can act as in vitro biological and in vivo pharmacological agents. However, further studies are needed to uncover individual responsible compounds of the observed biological potentials which would be helpful in devising novel drugs.
The Effect of Metal Cations on the Aqueous Behavior of Dopamine. Thermodynamic Investigation of the Binary and Ternary Interactions with Cd2+, Cu2+ and UO22+ in NaCl at Different Ionic Strengths and Temperatures
Authors: Gigliuto Antonio , Cigala Rosalia Maria , Irto Anna , Felice Maria Rosa , Pettignano Alberto , De Stefano Concetta , Crea Francesco
Published: December 2021

The interactions of dopamine [2-(3,4-Dihydroxyphenyl)ethylamine, (Dop−)] with cadmium(II), copper(II) and uranyl(VI) were studied in NaCl(aq) at different ionic strengths (0 ≤ I/mol dm−3 ≤ 1.0) and temperatures (288.15 ≤ T/K ≤ 318.15). From the elaboration of the experimental data, it was found that the speciation models are featured by species of different stoichiometry and stability. In particular for cadmium, the formation of only MLH, ML and ML2 (M = Cd2+; L = dopamine) species was obtained. For uranyl(VI) (UO22+), the speciation scheme is influenced by the use of UO2(acetate)2 salt as a chemical; in this case, the formation of ML2, MLOH and the ternary MLAc (Ac = acetate) species in a wide pH range was observed. The most complex speciation model was obtained for the interaction of Cu2+ with dopamine; in this case we observed the formation of the following species: ML2, M2L, M2L2, M2L2(OH)2, M2LOH and ML2OH. These speciation models were determined at each ionic strength and temperature investigated. As a further contribution to this kind of investigation, the ternary interactions of dopamine with UO22+/Cd2+ and UO22+/Cu2+ were investigated at I = 0.15 mol dm−3 and T = 298.15K. These systems have different speciation models, with the MM’L and M2M’L2OH [M = UO22+; M’ = Cd2+ or Cu2+, L = dopamine] common species; the species of the mixed Cd2+ containing system have a higher stability with respect the Cu2+ containing one. The dependence on the ionic strength of complex formation constants was modelled by using both an extended Debye–Hückel equation that included the Van’t Hoff term for the calculation of the formation enthalpy change values and the Specific Ion Interaction Theory (SIT). The results highlighted that, in general, the entropy is the driving force of the process. The quantification of the effective sequestering ability of dopamine towards the studied cations was evaluated by using a Boltzmann-type equation and the calculation of pL0.5 parameter. The sequestering ability was quantified at different ionic strengths, temperatures and pHs, and this resulted, in general, that the pL0.5 trend was always: UO22+ > Cu2+ > Cd2+.
Theoretical Investigation of Carbon Dioxide Adsorption on Li+-Decorated Nanoflakes
Authors: Petrushenko Igor K. , Ivanov Nikolay A. , Petrushenko Konstantin B.
Published: December 2021

Recently, the capture of carbon dioxide, the primary greenhouse gas, has attracted particular interest from researchers worldwide. In the present work, several theoretical methods have been used to study adsorption of CO2 molecules on Li+-decorated coronene (Li+@coronene). It has been established that Li+ can be strongly anchored on coronene, and then a physical adsorption of CO2 will occur in the vicinity of this cation. Moreover, such a decoration has substantially improved interaction energy (Eint) between CO2 molecules and the adsorbent. One to twelve CO2 molecules per one Li+ have been considered, and their Eint values are in the range from −5.55 to −16.87 kcal/mol. Symmetry-adapted perturbation theory (SAPT0) calculations have shown that, depending on the quantity of adsorbed CO2 molecules, different energy components act as the main reason for attraction. AIMD simulations allow estimating gravimetric densities (GD, wt.%) at various temperatures, and the maximal GDs have been calculated to be 9.3, 6.0, and 4.9% at T = 77, 300, and 400 K, respectively. Besides this, AIMD calculations validate stability of Li+@coronene complexes during simulation time at the maximum CO2 loading. Bader’s atoms-in-molecules (QTAIM) and independent gradient model (IGM) techniques have been implemented to unveil the features of interactions between CO2 and Li+@coronene. These methods have proved that there exists a non-covalent bonding between the cation center and CO2. We suppose that findings, derived in this theoretical work, may also benefit the design of novel nanosystems for gas storage and delivery.
Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture
Authors: Santin Anna , Russo Monia Teresa , Ferrante Maria Immacolata , Balzano Sergio , Orefice Ida , Sardo Angela
Published: December 2021

Microalgae have a great potential for the production of healthy food and feed supplements. Their ability to convert carbon into high-value compounds and to be cultured in large scale without interfering with crop cultivation makes these photosynthetic microorganisms promising for the sustainable production of lipids. In particular, microalgae represent an alternative source of polyunsaturated fatty acids (PUFAs), whose consumption is related to various health benefits for humans and animals. In recent years, several strategies to improve PUFAs’ production in microalgae have been investigated. Such strategies include selecting the best performing species and strains and the optimization of culturing conditions, with special emphasis on the different cultivation systems and the effect of different abiotic factors on PUFAs’ accumulation in microalgae. Moreover, developments and results obtained through the most modern genetic and metabolic engineering techniques are described, focusing on the strategies that lead to an increased lipid production or an altered PUFAs’ profile. Additionally, we provide an overview of biotechnological applications of PUFAs derived from microalgae as safe and sustainable organisms, such as aquafeed and food ingredients, and of the main techniques (and their related issues) for PUFAs’ extraction and purification from microalgal biomass.
Dragon Fruit Foliage: An Agricultural Cellulosic Source to Extract Cellulose Nanomaterials
Authors: Anh Tuyet Phung Thi , Nguyen Toan Viet , Hoang Phuong Thi , Thi Phuong Vu , Kim Thoa Nguyen , Van Quyen Nguyen , Van Chien Nguyen , Hai Yen Dao
Published: December 2021

In this report, we focus our effort to extract cellulose nanomaterials (CNs) from an agricultural cellulosic waste, Dragon Fruit foliage (DFF). DFF was first pretreated by several mechanical treatments and then bleached by chemical treatment to obtain bleached DFF. CNs were then produced from the hydrolysis of the bleached DFF catalyzed by sulfuric acid. We obtained CNs with a small diameter (50 to 130 nm) and length (100 to 500 nm) and a height of 3 to 10 nm. The CNs have a high crystallinity (crystallinity index 84.8%), high −COOH content (0.74 mmol·g−1), good thermal stability and a good Cu (II) adsorption capacity with an adsorption maximum of ~103 mg·g−1. These findings demonstrated the great potential of converting many agricultural cellulosic wastes into valuable cellulose nanomaterials.
Effects of Fe-Ions Irradiation on the Microstructure and Mechanical Properties of FeCrAl-1.5wt.% ZrC Alloys
Authors: Wang Runzhong , Wang Hui , Zhu Xiaohui , Liang Xue , Li Yuanfei , Gao Yunxia , An Xuguang , Liu Wenqing
Published: December 2021

Fe-13Cr-3.5Al-2.0Mo-1.5wt.% ZrC alloy was irradiated by 400 keV Fe+ at 400 °C at different doses ranging from 6.35 × 1014 to 1.27 × 1016 ions/cm2 with a corresponding damage of 1.0–20.0 dpa, respectively, to investigate the effects of different radiation doses on the hardness and microstructure of the reinforced FeCrAl alloys in detail by nanoindentation, transmission electron microscopy (TEM), and atom probe tomography (APT). The results show that the hardness at 1.0 dpa increases from 5.68 to 6.81 GPa, which is 19.9% higher than a non-irradiated specimen. With an increase in dose from 1.0 to 20.0 dpa, the hardness increases from 6.81 to 8.01 GPa, which is an increase of only 17.6%, indicating that the hardness has reached saturation. TEM and APT results show that high-density nano-precipitates and low-density dislocation loops forme in the 1.0 dpa region, compared to the non-irradiated region. Compared with 1.0 dpa region, the density and size of nano-precipitates in the 20.0 dpa region have no significant change, while the density of dislocation loops increases. Irradiation results in a decrease of molybdenum and carbon in the strengthening precipitates (Zr, Mo) (C, N), and the proportionate decrease of molybdenum and carbon is more obvious with the increase in damage.
Lignin–Cellulose Nanocrystals from Hemp Hurd as Light-Coloured Ultraviolet (UV) Functional Filler for Enhanced Performance of Polyvinyl Alcohol Nanocomposite Films
Authors: Zhang Yi , Haque Abu Naser Md Ahsanul , Naebe Maryam
Published: December 2021

Lignin is a natural light-coloured ultraviolet (UV) absorber; however, conventional extraction processes usually darken its colour and could be detrimental to its UV-shielding ability. In this study, a sustainable way of fabricating lignin–cellulose nanocrystals (L-CNCs) from hemp hurd is proposed. A homogeneous morphology of the hemp particles was achieved by ball milling, and L-CNCs with high aspect ratio were obtained through mild acid hydrolysis on the ball-milled particles. The L-CNCs were used as filler in polyvinyl alcohol (PVA) film, which produced a light-coloured nanocomposite film with high UV-shielding ability and enhanced tensile properties: the absorption of UV at wavelength of 400 nm and transparency in the visible-light region at wavelength of 550 nm was 116 times and 70% higher than that of pure PVA, respectively. In addition to these advantages, the nanocomposite film showed a water vapour transmission property comparable with commercial food package film, indicating potential applications.
A High-Performance Coniform Helmholtz Resonator-Based Triboelectric Nanogenerator for Acoustic Energy Harvesting
Authors: Yuan Haichao , Yu Hongyong , Liu Xiangyu , Zhao Hongfa , Zhang Yiping , Xi Ziyue , Zhang Qiqi , Liu Ling , Lin Yejin , Pan Xinxiang , Xu Minyi
Published: December 2021

Harvesting acoustic energy in the environment and converting it into electricity can provide essential ideas for self-powering the widely distributed sensor devices in the age of the Internet of Things. In this study, we propose a low-cost, easily fabricated and high-performance coniform Helmholtz resonator-based Triboelectric Nanogenerator (CHR-TENG) with the purpose of acoustic energy harvesting. Output performances of the CHR-TENG with varied geometrical sizes were systematically investigated under different acoustic energy conditions. Remarkably, the CHR-TENG could achieve a 58.2% higher power density per unit of sound pressure of acoustic energy harvesting compared with the ever-reported best result. In addition, the reported CHR-TENG was demonstrated by charging a 1000 μF capacitor up to 3 V in 165 s, powering a sensor for continuous temperature and humidity monitoring and lighting up as many as five 0.5 W commercial LED bulbs for acoustic energy harvesting. With a collection features of high output performance, lightweight, wide frequency response band and environmental friendliness, the cleverly designed CHR-TENG represents a practicable acoustic energy harvesting approach for powering sensor devices in the age of the Internet of Things.
Development and In Vitro/In Vivo Evaluation of pH-Sensitive Polymeric Nanoparticles Loaded Hydrogel for the Management of Psoriasis
Authors: Asad Muhammad Imran , Khan Dildar , Rehman Asim ur , Elaissari Abdelhamid , Ahmed Naveed
Published: December 2021

Methotrexate (MTX), the gold standard against psoriasis, poses severe problems when administered systemically viz increased toxicity, poor solubility and adverse reactions. Hence, a topical formulation of MTX for the management of psoriasis can be an effective approach. The present study aimed to develop an MTX based nanoparticle-loaded chitosan hydrogel for evaluating its potential efficacy in an imiquimod-induced psoriatic mice model. MTX-NPs loaded hydrogel was prepared and optimized using the o/w emulsion solvent evaporation method. Particle size, zeta potential, entrapment efficiency, in vitro drug release, ex vivo permeation, skin irritation and deposition studies were performed. Psoriatic Area and Severity Index (PASI) score/histopathological examinations were conducted to check the antipsoriatic potential of MTX-NPs loaded hydrogel using an imiquimod (IMQ)-induced psoriatic model. Optimized MTX-NPs showed a particle size of 256.4 ± 2.17 nm and encapsulation efficiency of 86 ± 0.03%. MTX-NPs loaded hydrogel displayed a 73 ± 1.21% sustained drug release in 48 h. Ex vivo permeation study showed only 19.95 ± 1.04 µg/cm2 of drug permeated though skin in 24 h, while epidermis retained 81.33% of the drug. A significant decrease in PASI score with improvement to normalcy of mice skin was observed. The developed MTX-NPs hydrogel displayed negligible signs of mild hyperkeratosis and parakeratosis, while histopathological studies showed healing signs of mice skin. So, the MTX-NPs loaded hydrogel can be a promising delivery system against psoriasis.
Solid Oxide Cell Electrode Nanocomposites Fabricated by Inkjet Printing Infiltration of Ceria Scaffolds
Authors: Anelli Simone , Moreno-Sanabria Luis , Baiutti Federico , Torrell Marc , Tarancón Albert
Published: December 2021

The enhancement of solid oxide cell (SOC) oxygen electrode performance through the generation of nanocomposite electrodes via infiltration using wet-chemistry processes has been widely studied in recent years. An efficient oxygen electrode consists of a porous backbone and an active catalyst, which should provide ionic conductivity, high catalytic activity and electronic conductivity. Inkjet printing is a versatile additive manufacturing technique, which can be used for reliable and homogeneous functionalization of SOC electrodes via infiltration for either small- or large-area devices. In this study, we implemented the utilization of an inkjet printer for the automatic functionalization of different gadolinium-doped ceria scaffolds, via infiltration with ethanol:water-based La1−xSrxCo1−yFeyO3−δ (LSCF) ink. Scaffolds based on commercial and mesoporous Gd-doped ceria (CGO) powders were used to demonstrate the versatility of inkjet printing as an infiltration technique. Using yttrium-stabilized zirconia (YSZ) commercial electrolytes, symmetrical LSCF/LSCF–CGO/YSZ/LSCF–CGO/LSCF cells were fabricated via infiltration and characterized by SEM-EDX, XRD and EIS. Microstructural analysis demonstrated the feasibility and reproducibility of the process. Electrochemical characterization lead to an ASR value of ≈1.2 Ω cm2 at 750 °C, in the case of nanosized rare earth-doped ceria scaffolds, with the electrode contributing ≈0.18 Ω cm2. These results demonstrate the feasibility of inkjet printing as an infiltration technique for SOC fabrication.
Efficient Achromatic Broadband Focusing and Polarization Manipulation of a Novel Designed Multifunctional Metasurface Zone Plate
Authors: Ge Shaobo , Liu Weiguo , Sun Xueping , Zhang Jin , Yang Pengfei , Xi Yingxue , Zhou Shun , Zhu Yechuan , Pu Xinxin
Published: December 2021

In this paper, comprehensively utilizing the diffraction theory and electromagnetic resonance effect is creatively employed to design a multifunctional metasurface zone plate (MMZP) and achieve the control of polarization states, while maintaining a broadband achromatic converging property in a near-IR region. The MMZP consists of several rings with fixed width and varying heights; each ring has a number of nanofins (usually called meta-atoms). The numerical simulation method is used to analyze the intensity distribution and polarization state of the emergent light, and the results show that the designed MMZP can realize the polarization manipulation while keeping the broadband in focus. For a specific design wavelength (0.7 μm), the incident light can be converted from left circularly polarized light to right circularly polarized light after passing through the MMZP, and the focusing efficiency reaches above 35%, which is more than twice as much as reported in the literature. Moreover, the achromatic broadband focusing property of the MMZP is independent with the polarization state of the incident light. This approach broadens degrees of freedom in micro-nano optical design, and is expected to find applications in multifunctional focusing devices and polarization imaging.
Polymer Micro and Nanoparticles Containing B(III) Compounds as Emissive Soft Materials for Cargo Encapsulation and Temperature-Dependent Applications
Authors: Duarte Frederico , Cuerva Cristián , Fernández-Lodeiro Carlos , Fernández-Lodeiro Javier , Jiménez Raquel , Cano Mercedes , Lodeiro Carlos
Published: December 2021

Polymer nanoparticles doped with fluorescent molecules are widely applied for biological assays, local temperature measurements, and other bioimaging applications, overcoming several critical drawbacks, such as dye toxicity, increased water solubility, and allowing imaging of dyes/drug delivery in water. In this work, some polymethylmethacrylate (PMMA), polyvinylpyrrolidone (PVP) and poly(styrene-butadiene-styrene) (SBS) based micro and nanoparticles with an average size of about 200 nm and encapsulating B(III) compounds have been prepared via the reprecipitation method by using tetrahydrofuran as the oil phase and water. The compounds are highly hydrophobic, but their encapsulation into a polymer matrix allows obtaining stable colloidal dispersions in water (3.39 µM) that maintain the photophysical behavior of these dyes. Although thermally activated non-radiative processes occur by increasing temperature from 25 to 80 °C, the colloidal suspension of the B(III) particles continues to emit greenish light (λ = 509 nm) at high temperatures. When samples are cooling back to room temperature, the emission is restored, being reversible. A probe of concept drug delivery study was conducted using coumarin 6 as a prototype of a hydrophobic drug.
Microfluidics Technology for the Design and Formulation of Nanomedicines
Authors: Jaradat Eman , Weaver Edward , Meziane Adam , Lamprou Dimitrios A.
Published: December 2021

In conventional drug administration, drug molecules cross multiple biological barriers, distribute randomly in the tissues, and can release insufficient concentrations at the desired pathological site. Controlling the delivery of the molecules can increase the concentration of the drug in the desired location, leading to improved efficacy, and reducing the unwanted effects of the molecules under investigation. Nanoparticles (NPs), have shown a distinctive potential in targeting drugs due to their unique properties, such as large surface area and quantum properties. A variety of NPs have been used over the years for the encapsulation of different drugs and biologics, acting as drug carriers, including lipid-based and polymeric NPs. Applying NP platforms in medicines significantly improves the disease diagnosis and therapy. Several conventional methods have been used for the manufacturing of drug loaded NPs, with conventional manufacturing methods having several limitations, leading to multiple drawbacks, including NPs with large particle size and broad size distribution (high polydispersity index), besides the unreproducible formulation and high batch-to-batch variability. Therefore, new methods such as microfluidics (MFs) need to be investigated more thoroughly. MFs, is a novel manufacturing method that uses microchannels to produce a size-controlled and monodispersed NP formulation. In this review, different formulation methods of polymeric and lipid-based NPs will be discussed, emphasizing the different manufacturing methods and their advantages and limitations and how microfluidics has the capacity to overcome these limitations and improve the role of NPs as an effective drug delivery system.
Ink Formulation and Printing Parameters for Inkjet Printing of Two Dimensional Materials: A Mini Review
Authors: Jun Ho-Young , Kim Se-Jung , Choi Chang-Ho
Published: December 2021

Inkjet printing of two-dimensional (2D) material has been a center of interest for wearable electronics and has become a promising platform for next-generation technologies. Despite the enormous progress made in printed 2D materials, there are still challenges in finding the optimal printing conditions involving the ink formulation and printing parameters. Adequate ink formulation and printing parameters for target 2D materials rely on empirical studies and repeated trials. Therefore, it is essential to compile promising strategies for ink formulation and printing parameters. In this context, this review discusses the optimal ink formulations to prepare stable ink and steady ink jetting and then explores the critical printing parameters for fabricating printed 2D materials of a high quality. The summary and future prospects for inkjet-printed 2D materials are also addressed.
The Effect of Silicon Grade and Electrode Architecture on the Performance of Advanced Anodes for Next Generation Lithium-Ion Cells
Authors: Meyer Alexandra , Ball Fabian , Pfleging Wilhelm
Published: December 2021

To increase the specific capacity of anodes for lithium-ion cells, advanced active materials, such as silicon, can be utilized. Silicon has an order of magnitude higher specific capacity compared to the state-of-the-art anode material graphite; therefore, it is a promising candidate to achieve this target. In this study, different types of silicon nanopowders were introduced as active material for the manufacturing of composite silicon/graphite electrodes. The materials were selected from different suppliers providing different grades of purity and different grain sizes. The slurry preparation, including binder, additives, and active material, was established using a ball milling device and coating was performed via tape casting on a thin copper current collector foil. Composite electrodes with an areal capacity of approximately 1.70 mAh/cm² were deposited. Reference electrodes without silicon were prepared in the same manner, and they showed slightly lower areal capacities. High repetition rate, ultrafast laser ablation was applied to these high-power electrodes in order to introduce line structures with a periodicity of 200 µm. The electrochemical performance of the anodes was evaluated as rate capability and operational lifetime measurements including pouch cells with NMC 622 as counter electrodes. For the silicon/graphite composite electrodes with the best performance, up to 200 full cycles at a C-rate of 1C were achieved until end of life was reached at 80% relative capacity. Additionally, electrochemical impedance spectroscopies were conducted as a function of state of health to correlate the used silicon grade with solid electrolyte interface (SEI) formation and charge transfer resistance values.
Behaviour of PMMA Resin Composites Incorporated with Nanoparticles or Fibre following Prolonged Water Storage
Authors: Alhotan Abdulaziz , Yates Julian , Zidan Saleh , Haider Julfikar , Jurado Carlos Alberto , Silikas Nikolaos
Published: December 2021

When PMMA denture base acrylics are exposed to oral environments for prolonged periods, the denture base absorbs water, which has a negative influence on the denture material and the degree to which the denture base will be clinically effective. This study assessed the water sorption, desorption, and hygroscopic expansion processes within PMMA denture-base resins reinforced with nanoparticles or fibre in comparison to the non-reinforced PMMA. The surfaces of the fillers were modified using a silane coupling agent (y-MPS) before mixing with PMMA. Group C consisted of specimens of pure PMMA whereas groups Z, T, and E consisted of PMMA specimens reinforced with ZrO2, TiO2 nanoparticles, or E-glass fibre, respectively. The reinforced groups were subdivided into four subgroups according to the percentage filler added to the PMMA resin by weight (1.5%, 3.0%, 5.0%, or 7.0%). Five specimens in disc shape (25 ± 1 mm × 2.0 ± 0.2 mm) were tested for each group. To assess water sorption and hygroscopic expansion, specimens from each group were individually immersed in water at 37 ± 1 °C for 180 days. The samples were then desorbed for 28 days at 37 ± 1 °C, to measure solubility. Water sorption and solubility were calculated using an electronic balance in accordance with ISO Standard 20795-1, and hygroscopic expansion was measured using a laser micrometre. Statistical analysis was undertaken at a p ≤ 0.05 significance level using a one-way ANOVA followed by Tukey post-hoc tests. The results demonstrated that the values of sorption (Wsp), mass sorption (Ms%), and % expansion within the tested groups reached equilibrium within 180 days. A noticeable difference was observed in groups Z and E for (Wsp)/(Ms%) compared to the Group C, but this was not significant. However, the difference between Group C and Group T for these measurements was significant. Non-significant differences also existed between each respective reinforced group and the control group in terms of hygroscopic expansion % values. During the 28-day desorption period, there were no differences in the values of solubility (Wsl)/mass desorption (Md%) between Group C and each of the reinforced tested groups. The findings indicate that the inclusion of ZrO2 nanoparticles or E-glass fibres does not increase the water solubility/sorption of the PMMA. However, modifying the PMMA with TiO2 did significantly increase the water sorption level.
Fermentable Oligo-, Di-, and Mono-Saccharides and Polyols (FODMAPs) Consumption and Irritable Bowel Syndrome in the French NutriNet-Santé Cohort
Authors: Schneider Elodie , Sabaté Jean-Marc , Bouchoucha Michel , Hercberg Serge , Touvier Mathilde , Benamouzig Robert , Julia Chantal , Buscail Camille
Published: December 2021

(1) Background: Specific foods, and more particularly, fermentable oligo-, di-, and mono-saccharides and polyols (FODMAPs) are often considered as triggers of digestive symptoms in Irritable Bowel Syndrome (IBS). Our aim was to study FODMAP consumption in controls and IBS participants in a large French population-based cohort; (2) Methods: Participants from the NutriNet-Santé cohort study completed the Rome IV and IBS-SSS questionnaire in a cross sectional study. Among them, 27,949 eligible participants had previously completed three 24-h recalls as well as anthropometrics, socio-demographical and lifestyle data. Total FODMAP intake (in g/day) was computed using a specific composition table. The association between FODMAPs and IBS was estimated through multivariable logistic regression models; (3) Results: Included participants were mainly women (75.4%) and the mean age was 43.4 ± 14.1 years. FODMAPs accounted for a mean daily intake of 19.4 ± 9.5 g/day. Overall 1295 participants (4.6%) were identified with an IBS. After adjusting for confounding factors, IBS participants had lower intakes in FODMAPs than non-IBS ones (aOR: 0.88, 95% CI: 0.82–0.95, p-value: 0.001). IBS severity was associated with more frequent low FODMAP intakes (<9 g/day); (4) Conclusions: Participants tended to consume 19 g of FODMAPs per day, but slightly less for IBS participants than for controls. In IBS participants, higher severity was associated with lower intakes.
Comment on Kim et al. The Association between Coffee Consumption and Risk of Colorectal Cancer in a Korean Population. Nutrients 2021, 13, 2753
Authors: Dhawan Ravi , Zhao Yuchen , Giovannucci Edward , Smith-Warner Stephanie
Published: December 2021

We read with interest the recent publication in Nutrients by Kim et al. [...]
The Australian Feeding Infants and Toddlers Study (OzFITS) 2021: Study Design, Methods and Sample Description
Authors: Moumin Najma A. , Golley Rebecca K. , Mauch Chelsea E. , Makrides Maria , Green Tim J. , Netting Merryn J.
Published: December 2021

(1) Background: Caregiver feeding practices during the first two years of a child’s life influence nutrition, growth, and development, as well as long term taste preferences and dietary patterns. Suboptimal feeding practices lead to poorer health outcomes, such as obesity, that persist into adulthood. Although the importance of early life nutrition is well-established, there are no Australia-wide surveys of dietary intakes of children under two years of age. The 2021 Australian Feeding Infants and Toddlers Study (OzFITS) aims to fill this gap. This paper describes the methods and study sample of OzFITS 2021. (2) Methods: OzFITS 2021 is a cross-sectional study of children aged 0 to 23.9 months of age and their caregiver across Australia. Data were collected between April 2020 and April 2021. A telephone-based survey was completed with a caregiver to obtain information on child and caregiver characteristics and feeding practices. For exclusively breastfed infants, the number of breastfeeds in a 24 h period was reported. Dietary intakes for mixed fed children were estimated using a one-day food record, with 30% of caregivers completing a second food record on a non-consecutive day. (3) Results: We enrolled 1140 caregiver and child dyads. Of those eligible to complete a food record, 853 (87%) completed the food record. Compared to the Australian population, caregivers were more likely to be university-educated (>75%), married or in a de facto relationship (94%), and have a household income >$100,000/y (60%). (4) Conclusions: OzFITS 2021 is the first national study to examine food and nutrient intake in Australian children aged under 2 years. The study will provide information on breastfeeding rates and duration, use of breast milk substitutes, and timing of solid food introduction. Dietary intake data will allow the comparison of core food groups and discretionary food intake to Australian guidelines and estimate the prevalence of inadequate intake of key nutrients, like iron. Healthcare practitioners and policymakers can use the study findings as a source of evidence to inform the next iteration of infant feeding guidelines.
Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging—The Effects of Long-Term Food Restriction
Authors: Tesic Vesna , Ciric Jelena , Jovanovic Macura Irena , Zogovic Nevena , Milanovic Desanka , Kanazir Selma , Perovic Milka
Published: December 2021

Numerous beneficial effects of food restriction on aging and age-related pathologies are well documented. It is also well-established that both short- and long-term food restriction regimens induce elevated circulating levels of glucocorticoids, stress-induced hormones produced by adrenal glands that can also exert deleterious effects on the brain. In the present study, we examined the effect of long-term food restriction on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the cortex during aging, in 18- and 24-month-old rats. Corticosterone level was increased in the cortex of aged ad libitum-fed rats. Food restriction induced its further increase, accompanied with an increase in the level of 11β-hydroxysteroid dehydrogenase type 1. However, alterations in the level of GR phosphorylated at Ser232 were not detected in animals on food restriction, in line with unaltered CDK5 level, the decrease of Hsp90, and an increase in a negative regulator of GR function, FKBP51. Moreover, our data revealed that reduced food intake prevented age-related increase in the levels of NFκB, gfap, and bax, confirming its anti-inflammatory and anti-apoptotic effects. Along with an increase in the levels of c-fos, our study provides additional evidences that food restriction affects cortical responsiveness to glucocorticoids during aging.
Innovative Application of Phytochemicals from Fermented Legumes and Spices/Herbs Added in Extruded Snacks
Authors: Szymandera-Buszka Krystyna , Gumienna Małgorzata , Jędrusek-Golińska Anna , Waszkowiak Katarzyna , Hęś Marzanna , Szwengiel Artur , Gramza-Michałowska Anna
Published: December 2021

A trend related to adding legume seeds to various products has been observed. This work aimed to use fermented red bean/broad bean seeds and their hulls to produce extruded snacks with more beneficial nutritional properties and good sensory quality. Extruded snacks containing fermented ground seeds (50%) or hull (10%) of red bean/broad bean and corn grits with the addition of selected herbs/spices (0.5%) were prepared. The chemical composition, phenolic profile, antioxidant activity, and sensory quality were analysed. The results showed that the protein content ranged from 9 to 22.9 g 100 g−1, phenolic compounds ranged from 3.97 to 12.80 mg 100 g−1 (with the addition of herbs/spices, even up to 62.88 mg 100 g−1), and antioxidant activities ranged from 4.32 to 10.23 Trolox g−1 (ABTS assay), depending on the type of fermented materials. The addition of ground seeds/hull did not influence the consumer desirability, whereas the addition of selected herbs/spices, particularly lovage, increased it. The application of fermented red bean and broad bean seeds and their hulls, as part of the assumptions of the planetary diet, enabled enrichment of extruded corn products, which are often consumed by vegans and vegetarians, with nutritionally valuable ingredients.
Threshold of Reactivity and Tolerance to Precautionary Allergen-Labelled Biscuits of Baked Milk- and Egg-Allergic Children
Authors: Fierro Vincenzo , Marzano Valeria , Monaci Linda , Vernocchi Pamela , Mennini Maurizio , Valluzzi Rocco , Levi Mortera Stefano , Pilolli Rosa , Dahdah Lamia , Calandrelli Veronica , Bracaglia Giorgia , Arasi Stefania , Riccardi Carla , Fiocchi Alessandro , Putignani Lorenza
Published: December 2021

Extremely sensitive food-allergic patients may react to very small amounts of allergenic foods. Precautionary allergen labelling (PAL) warns from possible allergenic contaminations. We evaluated by oral food challenge the reactivity to a brand of PAL-labelled milk- and egg-free biscuits of children with severe milk and egg allergy. We explored the ability of proteomic methods to identify minute amounts of milk/egg allergens in such biscuits. Traces of milk and/or egg allergens in biscuits were measured by two different liquid-chromatography-mass spectrometry methods. The binding of patient’s serum with egg/milk proteins was assessed using immunoblotting. None of the patients reacted to biscuits. Egg and milk proteins were undetectable with a limit of detection of 0.6 µg/g for milk and egg (method A), and of 0.1 and 0.3 µg /g for milk and egg, respectively (method B). The immunoblots did not show milk/egg proteins in the studied biscuits. Milk/egg content of the biscuits is far lower than 4 µg of milk or egg protein per gram of product, the minimal doses considered theoretically capable of causing reactions. With high sensitivity, proteomic assessments predict the harmlessness of very small amount of allergens in foods, and can be used to help avoiding unnecessary PAL.
Tetrahydrocurcumin Upregulates the Adiponectin-AdipoR Pathway and Improves Insulin Signaling and Pancreatic β-Cell Function in High-Fat Diet/Streptozotocin-Induced Diabetic Obese Mice
Authors: Tsai Yi-Zhen , Tsai Mei-Ling , Hsu Li-Yin , Ho Chi-Tang , Lai Ching-Shu
Published: December 2021

Impairment of adiponectin production and function is closely associated with insulin resistance and type 2 diabetes, which are linked to obesity. Studies in animal models have documented the anti-diabetic effects of tetrahydrocurcumin (THC). Although several possible mechanisms have been proposed, the contribution of adiponectin signaling on THC-mediated antihyperglycemic effects remains unknown. Here, we report that adiposity, steatosis, and hyperglycemia were potently attenuated in high-fat diet/streptozotocin-induced diabetic obese mice after they received 20 and 100 mg/kg THC for 14 weeks. THC upregulated UCP-1 in adipose tissue and elevated adiponectin levels in the circulation. THC upregulated the AdipoR1/R2-APPL1-mediated pathway in the liver and skeletal muscle, which contributes to improved insulin signaling, glucose utilization, and lipid metabolism. Furthermore, THC treatment significantly (p < 0.05) preserved islet mass, reduced apoptosis, and restored defective insulin expression in the pancreatic β-cells of diabetic obese mice, which was accompanied by an elevation of AdipoR1 and APPL1. These results demonstrated a potential mechanism underlying the beneficial effects of THC against hyperglycemia via the adiponectin-AdipoR pathway, and thus, may lead to a novel therapeutic use for type 2 diabetes.
Effects of Cigarette Smoking on Influenza Virus/Host Interplay
Authors: Chavez Jerald , Hai Rong
Published: December 2021

Cigarette smoking has been shown to increase the risk of respiratory infection, resulting in the exacerbation of infectious disease outcomes. Influenza viruses are a major respiratory viral pathogen, which are responsible for yearly epidemics that result in between 20,000 and 50,000 deaths in the US alone. However, there are limited general summaries on the impact of cigarette smoking on influenza pathogenic outcomes. Here, we will provide a systematic summarization of the current understanding of the interplay of smoking and influenza viral infection with a focus on examining how cigarette smoking affects innate and adaptive immune responses, inflammation levels, tissues that contribute to systemic chronic inflammation, and how this affects influenza A virus (IAV) disease outcomes. This summarization will: (1) help to clarify the conflict in the reports on viral pathogenicity; (2) fill knowledge gaps regarding critical anti-viral defenses such as antibody responses to IAV; and (3) provide an updated understanding of the underlying mechanism behind how cigarette smoking influences IAV pathogenicity.
Distribution and Genetic Diversity of Hepatitis E Virus in Wild and Domestic Rabbits in Australia
Authors: Jenckel Maria , Smith Ina , King Tegan , West Peter , Taggart Patrick L. , Strive Tanja , Hall Robyn N.
Published: December 2021

In 2020, Hepatitis E virus (HEV) was detected for the first time in Australian rabbits. To improve our understanding of the genetic diversity and distribution of the virus, 1635 rabbit liver samples from locations across Australia were screened via RT-qPCR for HEV. HEV genomes were amplified and sequenced from 48 positive samples. Furthermore, we tested 380 serum samples from 11 locations across Australia for antibodies against HEV. HEV was detected in rabbits from all states and territories, except the Northern Territory. Seroprevalence varied between locations (from 0% to 22%), demonstrating that HEV is widely distributed in rabbit populations across Australia. Phylogenetic analyses showed that Australian HEV sequences are genetically diverse and that HEV was likely introduced into Australia independently on several occasions. In summary, this study broadens our understanding of the genetic diversity of rabbit HEV globally and shows that the virus is endemic in both domestic and wild rabbit populations in Australia.
Validation and Application of Skin RT-QuIC to Patients in China with Probable CJD
Authors: Xiao Kang , Yang Xuehua , Zhou Wei , Chen Cao , Shi Qi , Dong Xiaoping
Published: December 2021

The definite diagnosis of human sporadic Creutzfeldt–Jakob disease (sCJD) largely depends on postmortem neuropathology and PrPSc detection in the brain. The development of real-time quaking-induced conversion (RT-QuIC) of cerebrospinal fluid (CSF) samples makes it possible for premortem diagnosis for sCJD. To test the diagnostic potential of RT-QuIC of skin specimens for probable sCJD, we collected the paired skin and CSF samples from 51 recruited living patients referred to the Chinese CJD surveillance center, including 34 probable sCJD, 14 non-CJD, and 3 genetic prion disease (gPrD). The samples were subjected to RT-QuIC assays using recombinant hamster PrP protein rHaPrP90-231 as the substrate. Using skin RT-QuIC assay, 91.2% (31/34) probable sCJD patients, and 1 T188K genetic CJD (gCJD) cases showed positive prion-seeding activity, while 85.7% (12/14) non-CJD patients were negative. CSF RT-QuIC positive seeding activity was only observed in 14 probable sCJD patients. Analysis of the reactivity of 38 positive skin RT-QuIC tests revealed that the positive rates in the preparations of 10−2, 10−3 and 10−4 diluted skin samples were 88.6% (39/44), 63.6% (28/44), and 25.0% (11/44), respectively. Eleven probable sCJD patients donated two skin specimens collected at different sites simultaneously. Although 95.5% (21/22) skin RT-QuIC elicited positive reaction, the reactivity varied. Our preliminary data indicate high sensitivity and specificity of skin RT-QuIC in prion detection for Chinese probable sCJD and highlight that skin prion-seeding activity is a reliable biomarker for premortem diagnosis of human prion disease.
Tryptophanyl tRNA Synthetase from Human Macrophages Infected by Porphyromonas gingivalis Induces a Proinflammatory Response Associated with Atherosclerosis
Authors: Sasaki Minoru , Shimoyama Yu , Kodama Yoshitoyo , Ishikawa Taichi
Published: December 2021

Porphyromonas gingivalis is the most common microorganism associated with adult periodontal disease, causing inflammation around the subgingival lesion. In this study, we investigated tryptophanyl tRNA synthase (WRS) production by THP-1 cells infected with P. gingivalis. Cytokine production, leukocyte adhesion molecules, and low-density lipoprotein receptor (LDLR) expressions in cultured cells were examined. WRS was detected in THP-1 cell culture supernatants stimulated with P. gingivalis from 1 to 24 h, and apparent production was observed after 4 h. No change in WRS mRNA expression was observed from 1 to 6 h in THP-1 cells, whereas its expression was significantly increased 12 h after stimulation with P. gingivalis. Lactate dehydrogenase (LDH) activity was observed from 4 to 24 h. The TNF-α, IL-6, IL-8, and CXCL2 levels of THP-1 cells were upregulated after treatment with recombinant WRS (rWRS) and were significantly reduced when THP-1 cells were treated with C29. The MCP-1, ICAM-1, and VCAM-1 levels in human umbilical vein endothelial cells were upregulated following treatment with rWRS, and TAK242 suppressed these effects. Additionally, unmodified LDLR, macrophage scavenger receptor A, and lectin-like oxidized LDLRs were upregulated in THP-1 cells treated with rWRS. These results suggest that WRS from macrophages infected with P. gingivalis is associated with atherosclerosis.
Protective Effect of Carotenoid Extract from Orange-Fleshed Sweet Potato on Gastric Ulcer in Mice by Inhibition of NO, IL-6 and PGE2 Production
Authors: Bae Ji-Yeong , Park Woo-Sung , Kim Hye-Jin , Kim Ho-Soo , Kang Kwon-Kyoo , Kwak Sang-Soo , Ahn Mi-Jeong
Published: December 2021

Ipomoea batatas (L.) Lam., Convolvulaceae is widely distributed in Asian areas from tropical to warm-temperature regions. Their tubers are known for their antioxidant, anti-bacterial, anti-diabetic, wound healing, anti-inflammatory, and anti-ulcer activities. The preventive and therapeutic effects of orange-fleshed sweet potato on gastric ulcers have not been investigated. In this study, the carotenoid extract (CE) of orange-fleshed sweet potato was found to protect against gastric ulcers induced by HCl/ethanol in mice. The anti-inflammatory and antioxidant activities of the carotenoid pigment extract were also evaluated as possible evidence of their protective effects. Administration of CE reduced gastric ulcers. Oral administration of CE (100 mg/kg) protected against gastric ulcers by 78.1%, similar to the positive control, sucralfate (77.5%). CE showed potent reducing power and decreased nitric oxide production in a mouse macrophage cell line, RAW 264.7, in a concentration-dependent manner. The production of the inflammatory cytokine interleukin-6 and prostaglandin E2 was also reduced by CE in a dose-dependent manner. The high carotenoid content of orange-fleshed sweet potato could play a role in its protective effect against gastric ulcers. This result suggests the possibility of developing functional products using this nutrient-fortified material.
2-(Piperidin-4-yl)acetamides as Potent Inhibitors of Soluble Epoxide Hydrolase with Anti-Inflammatory Activity
Authors: Martín-López Juan , Codony Sandra , Bartra Clara , Morisseau Christophe , Loza María Isabel , Sanfeliu Coral , Hammock Bruce D. , Brea José , Vázquez Santiago
Published: December 2021

The pharmacological inhibition of soluble epoxide hydrolase (sEH) has been suggested as a potential therapy for the treatment of pain and inflammatory diseases through the stabilization of endogenous epoxyeicosatrienoic acids. Numerous potent sEH inhibitors (sEHI) have been developed, however many contain highly lipophilic substituents limiting their availability. Recently, a new series of benzohomoadamantane-based ureas endowed with potent inhibitory activity for the human and murine sEH was reported. However, their very low microsomal stability prevented further development. Herein, a new series of benzohomoadamantane-based amides were synthetized, fully characterized, and evaluated as sEHI. Most of these amides were endowed with excellent inhibitory potencies. A selected compound displayed anti-inflammatory effects with higher effectiveness than the reference sEHI, TPPU.