bluuebunny/crossref_metadata_2025 huggingface dataset:
Adjustable Fully Adaptive Cross-Entropy Algorithms for Task Assignment of Multi-UAVs
Authors: Wang Kehao , Zhang Xun , Qiao Xuyang , Li Xiaobai , Cheng Wei , Cong Yirui , Liu Kezhong
Published: March 2023

This paper investigates the multiple unmanned aerial vehicle (multi-UAV) cooperative task assignment problem. Specifically, we assign different types of UAVs to accomplish the classification, attack, and verification tasks of targets under resource, precedence, and timing constraints. Due to complex coupling among these tasks, we decompose the considered problem into two subproblems: one with continuous and independent tasks and another with continuous and correlative tasks. To solve them, we first present an adjustable, fully adaptive cross-entropy (AFACE) algorithm based on the cross-entropy (CE) method, which serves as a stepping stone for developing other algorithms. Secondly, to overcome task precedence in the first subproblem, we propose a mutually independent AFACE (MIAFACE) algorithm, which converges faster than the CE method when obtaining the optimal scheme vectors of these continuous and independent tasks. Thirdly, to deal with task coupling in the second subproblem, we present a mutually correlative AFACE (MCAFACE) algorithm to find the optimal scheme vectors of these continuous and correlative tasks, while its computational complexity is inferior to that of the MIAFACE algorithm. Finally, numerical simulations demonstrate that the proposed MIAFACE (MCAFACE, respectively) algorithm consumes less time than the existing algorithms for the continuous and independent (correlative, respectively) task assignment problem.
KNN-Based Consensus Algorithm for Better Service Level Agreement in Blockchain as a Service (BaaS) Systems
Authors: Zheng Qingxiao , Wang Lingfeng , He Jin , Li Taiyong
Published: March 2023

With services in cloud manufacturing expanding, cloud manufacturers increasingly use service level agreements (SLAs) to guarantee business processing cooperation between CSPs and CSCs (cloud service providers and cloud service consumers). Although blockchain and smart contract technologies are critical innovations in cloud computing, consensus algorithms in Blockchain as a Service (BaaS) systems often overlook the importance of SLAs. In fact, SLAs play a crucial role in establishing clear commitments between a service provider and a customer. There are currently no effective consensus algorithms that can monitor the SLA and provide service level priority. To address this issue, we propose a novel KNN-based consensus algorithm that classifies transactions based on their priority. Any factor that impacts the priority of the transaction can be used to calculate the distance in the KNN algorithm, including the SLA definition, the smart contract type, the CSC type, and the account type. This paper demonstrates the full functionality of the enhanced consensus algorithm. With this new method, the CSP in BaaS systems can provide improved services to the CSC. Experimental results obtained by adopting the enhanced consensus algorithm show that the SLA is better satisfied in the BaaS systems.
Serious Games and Soft Skills in Higher Education: A Case Study of the Design of Compete!
Authors: McGowan Nadia , López-Serrano Aída , Burgos Daniel
Published: March 2023

This article describes the serious game Compete!, developed within the European Erasmus+ framework, that aims to teach soft skills to higher education students in order to increase their employability. Despite the increasing relevance of soft skills for successful entry into the labour market, these are often overlooked in higher education. A participatory learning methodology based on a gamification tool has been used for this purpose. The game presents a series of scenarios describing social sustainability problems that require the application of soft skills identified as key competencies in a field study across different European countries. These competencies are creative problem-solving, effective communication, stress management, and teamwork. On completion of each game scenario and the game itself, students receive an evaluation of both their soft skills and the strategic and operational decisions they have made. In the evaluation of these decisions, both the economic and sustainability aspects of the decision are assessed. The teacher can then address the competencies and sustainability issues using the different game scenarios, thus creating higher motivation and deeper understanding amongst the students. This hybrid learning methodology incorporates digital tools for the cross-curricular teaching and learning of sustainability and soft skills. In conclusion, this article describes a possible method of incorporating soft skills in higher education; this complements students’ technical knowledge while helping to achieve Sustainable Development Goals.
Beneš Network-Based Efficient Data Concentrator for Triggerless Data Acquisition Systems
Authors: Gumiński Marek , Kruszewski Michał , Zabołotny Bartosz Marek , Zabołotny Wojciech Marek
Published: March 2023

The concentration of data from multiple links to a single output is an essential task performed by High-Energy Physics (HEP) Data Acquisition Systems (DAQs). At high and varying data rates combined with the large width of the concentrator’s output interface, this task is non-trivial. A high-speed dense packing of data from possibly non-continuous streams with preserving their time order requires complex and real-time adjustable routing. This paper presents a concentrator based on the Beneš network, which provides efficient concentration without using a high-frequency clock internally. It warrants that empty data are eliminated and does not disturb the data time-ordering if the data rates significantly differ between inputs. The concentrator uses simple data-routing primitives resulting in low resource consumption. If necessary, the pipeline registers may be added after each routing stage, shortening the critical path and increasing the maximum acceptable clock frequency. These features render the design well-suited to FPGA implementation.
Characterizing Complex Deformation, Damage, and Fracture in Heterogeneous Shale Using 3D-DIC
Authors: Nath Fatick , Aguirre Gabriel , Aguirre Edgardo
Published: March 2023

Safe drilling and effective fracturing are constant challenges for shale formations. One of the most important influencing factors is the accurate characterization of the deformation and damage caused by inherent lamination and natural fractures. Furthermore, shale formations exhibit fine-scale heterogeneity, which conventional laboratory methods (linear variable differential transformer (LVDT), strain gauges, etc.) cannot distinguish. To overcome these constraints, this research aims to investigate the damage and deformation characteristics of shale samples using three-dimensional digital image correlation (3D-DIC). Under uniaxial and diametrical compression, samples of Wolfcamp, Mancos, and Eagle Ford shale with distinct lamination and natural fractures are evaluated. The 3D-DIC system is utilized for image processing, visualization, and analysis of the shale damage process under varying loads. DIC made quantitative full-field strain maps with load (tension, compression, and shear), showing all the damage process steps and strain localization zones (SLZs). DIC maps are used to quantify damage variables in order to investigate sample damage. Damage variables are used to categorize the damage evolution process of shale specimens into four stages: initial damage, linear elastic, elastic–plastic, and plastic damage. Characterizing shale damage evolution with a strain localization line is more effective because there is more damage there than in the whole sample. Damage variables based on major strain and its standard deviation from the DIC strain map for all tested shale samples follow a similar trend, though diametrical compression variables are greater than uniaxial compression. In both uniaxial and diametrical compression, the Wolfcamp shale was reported to have the highest damage variable, which was measured at 0.37, while the Eagle Ford shale was reported to have the lowest damage variable. This image-based technique is more effective not only for understanding the laminated and naturally fractured rocks but also for predicting the hydraulic fractures that will occur during the stimulation process.
Simulation of the Temperature of a Shielding Induction Motor of the Nuclear Main Pump under Different Turbulence Models
Authors: Ai Likun , Lu Yiping , Han Jiade , Suo Wenxu
Published: March 2023

Thermal design and the choice of turbulence models are crucial for motors. In this project, the geometrical model of the vertical shielding induction motor for a small nuclear main pump was established by SolidWorks software and the finite volume method was adopted to investigate the temperature of the motor, especially the temperatures of bearings lubricated water. To make the numerical simulation of flow and heat transfer in the rotating clearance of the shielding induction motor more accurate, the effects of four types of different two equation turbulence models on the temperature field of the shielding induction motor were studied. The results showed that different choices of turbulence models had little effect on the temperature of the winding insulation but influenced the temperature of the lower guide bearing lubricating water and the secondary cooling water outlet. The SST k-ω model showed the lowest relative error result of the temperature of the winding insulation and the bearing lubricating water in the primary loop system of the shielding induction motor. The temperature of the clearance water, the spiral tube water and the spiral groove water increased approximately linearly along the axial direction.
Assessment of Physical and Mechanical Properties Considering the Stem Height and Cross-Section of Paulownia tomentosa (Thunb.) Steud. x elongata (S.Y.Hu) Wood
Authors: Barbu Marius Cătălin , Tudor Eugenia Mariana , Buresova Katharina , Petutschnigg Alexander
Published: March 2023

The aim of this study is to analyze the properties of Paulownia tomentosa x elongata plantation wood from Serbia, considering the influence of the stem height (0 to 1 m and 4.5 to 6 m above soil level—height spot) and radial position from the pith to bark (in the core, near the bark, and in between these zones—cross-section spot). The results show that most properties are improved when the samples were taken from upper parts of the tree (height spot) and from the near bark spot (cross-section spot). The mean density measured 275 kg/m3 at the stem height between 4.5–6 m and 245 kg/m3 for the samples collected from 0–1 m trunk height. The density had the highest value on the spot near bark (290 kg/m3), for the mature wood at a height of 4.5–6 m, and near pith had a mean density of 230 kg/m3. The Brinell hardness exhibited highest values in the axial direction (23 N/mm2) and near bark (28 N/mm2). The bending strength was 41 N/mm2 for the trunk’s height range of 4.5–6 m and 45 N/mm2 in the cross-section, close to cambium. The three-point modulus of elasticity (MOR) of the samples taken at a stem height of 4.5 to 6 m was up to 5000 N/mm2, and on the spot near bark, the MOR measured 5250 N/mm2. Regarding compressive strength, in the cross-section, near the pith, the mean value was the highest with 23 N/mm2 (4.5–6 m), whilst it was 19 N/mm2 near bark. The tensile strength was, on average, 40 N/mm2 for both 0–1 m and 4.5–6 m trunk height levels and 49 N/mm2 between bark and pith. The screw withdrawal resistance measured 58 N/mm for the samples extracted at a stem height of 4.5 to 6 m and 92 N/mm for the specimens collected near pith. This study stresses the influence, in short-rotation Paulownia timber, of indicators, such as juvenile and mature wood (difference emphasized after the fifth year of growth) and height variation, on the physical and mechanical properties of sawn wood. This study will help utilize more efficient sustainable resources, such as Paulownia plantation wood. This fast-growing hardwood species from Europe is adequate as a core material in sandwich applications for furniture, transport, sport articles, and lightweight composites, being considered the European Balsa.
Exploring the Inhibitory Activity of Selected Lactic Acid Bacteria against Bread Rope Spoilage Agents
Authors: Iosca Giovanna , Fugaban Joanna Ivy Irorita , Özmerih Süleyman , Wätjen Anders Peter , Kaas Rolf Sommer , Hà Quốc , Shetty Radhakrishna , Pulvirenti Andrea , De Vero Luciana , Bang-Berthelsen Claus Heiner
Published: March 2023

In this study, a wide pool of lactic acid bacteria strains deposited in two recognized culture collections was tested against ropy bread spoilage bacteria, specifically belonging to Bacillus spp., Paenibacillus spp., and Lysinibacillus spp. High-throughput and ex vivo screening assays were performed to select the best candidates. They were further investigated to detect the production of active antimicrobial metabolites and bacteriocins. Moreover, technological and safety features were assessed to value their suitability as biocontrol agents for the production of clean-label bakery products. The most prominent inhibitory activities were shown by four strains of Lactiplantibacillus plantarum (NFICC19, NFICC 72, NFICC163, and NFICC 293), two strains of Pediococcus pentosaceus (NFICC10 and NFICC341), and Leuconostoc citreum NFICC28. Moreover, the whole genome sequencing of the selected LAB strains and the in silico analysis showed that some of the strains contain operons for bacteriocins; however, no significant evidence was observed phenotypically.
Investigation of Geraniol Biotransformation by Commercial Saccharomyces Yeast Strains by Two Headspace Techniques: Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometry (PTR-ToF-MS)
Authors: Roberts Rebecca , Khomenko Iuliia , Eyres Graham T. , Bremer Phil , Silcock Patrick , Betta Emanuela , Biasioli Franco
Published: March 2023

Hop-derived volatile organic compounds (VOCs) and their transformation products significantly impact beer flavour and aroma. Geraniol, a key monoterpene alcohol in hops, has been reported to undergo yeast-modulated biotransformation into various terpenoids during fermentation, which impacts the citrus and floral aromas of the finished beer. This study monitored the evolution of geraniol and its transformation products throughout fermentation to provide insight into differences as a function of yeast species and strain. The headspace concentration of VOCs produced during fermentation in model wort was measured using Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry (SPME-GC/MS) and Proton Transfer Reaction-Time of Flight-Mass Spectrometry (PTR-ToF-MS). In the absence of yeast, only geraniol was detected, and no terpenoid compounds were detected in geraniol-free ferments. During fermentation, the depletion of geraniol was closely followed by the detection of citronellol, citronellyl acetate and geranyl acetate. The concentration of the products and formation behaviour was yeast strain dependent. SPME-GC/MS provided confidence in compound identification. PTR-ToF-MS allowed online monitoring of these transformation products, showing when formation differed between Saccharomyces cerevisiae and Saccharomyces pastorianus yeasts. A better understanding of the ability of different yeast to biotransform hop terpenes will help brewers predict, control, and optimize the aroma of the finished beer.
Complete Mitochondrial Genome and Phylogenetic Position of Chirolophis wui (Perciformes: Stichaeidae)
Authors: Lee Yong-Suk , Patil Maheshkumar Prakash , Kim Jong-Oh , Lee Yu-Jin , Seo Yong Bae , Kim Jin-Koo , Mahale Kiran R. , Kim Gun-Do
Published: March 2023

The complete mitochondrial genome of Chirolophis wui (Wang and Wang, 1935) was sequenced using the Illumina platform. The genome sequence is 16,522 bp in length with 54% A+T content and contains 13 protein coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and 1 control region (D-loop). The H-strand contains 28 genes (12 PCGs, 14 tRNAs, and 2 rRNAs), whereas the L-strand accommodates 9 genes (ND6 and 8 tRNAs). The nucleotide composition of the mitochondrial genome of C. wui is AT-biased, accounting for 54.0%, with an AT skew value of −0.0556 and a GC skew value of −0.2043. The majority of PCGs utilized the start codon, ATG, while only one gene, COI, utilized the alternative start codon, GTG. Of the 13 PCGs, 6 genes used the termination codon (TAA or TGA), whereas 7 genes used the incomplete termination codon (T or TA). Among the 22 tRNA genes, the tRNA-Leu and tRNA-Ser were found in duplicates. A phylogenetic tree was constructed using 10 complete mitochondrial genome sequences and indicated that C. wui has a very close relationship with C. japonicus and other species in the family Stichaeidae, with a high supporting bootstrap value. This study can provide valuable information for future evolutionary studies on C. wui and Stichaeidae.
A Review of Preconditioning and Artificial Compressibility Dual-Time Navier–Stokes Solvers for Multiphase Flows
Authors: Nguyen Van-Tu , Park Warn-Gyu
Published: March 2023

This review paper aims to summarize recent advancements in time-marching schemes for solving Navier–Stokes (NS) equations in multiphase flow simulations. The focus is on dual-time stepping, local preconditioning, and artificial compressibility methods. These methods have proven to be effective in achieving high time accuracy in simulations, as well as converting the incompressible NS equations into a hyperbolic form that can be solved using compact schemes, thereby accelerating the solution convergence and allowing for the simulation of compressible flows at all Mach numbers. The literature on these methods continues to grow, providing a deeper understanding of the underlying physical processes and supporting technological advancements. This paper also highlights the imposition of dual-time stepping on both incompressible and compressible NS equations. This paper provides an updated overview of advanced methods for the CFD community to continue developing methods and select the most suitable two-phase flow solver for their respective applications.
Meta-Analysis of In Vitro Antimicrobial Capacity of Extracts and Essential Oils of Syzygium aromaticum, Citrus L. and Origanum L.: Contrasting the Results of Different Antimicrobial Susceptibility Methods
Authors: Silva Beatriz Nunes , Bonilla-Luque Olga María , Possas Arícia , Ezzaky Youssef , Elmoslih Abdelkhaleq , Teixeira José António , Achemchem Fouad , Valero Antonio , Cadavez Vasco , Gonzales-Barron Ursula
Published: March 2023

Diffusion methods, including agar disk-diffusion and agar well-diffusion, as well as dilution methods such as broth and agar dilution, are frequently employed to evaluate the antimicrobial capacity of extracts and essential oils (EOs) derived from Origanum L., Syzygium aromaticum, and Citrus L. The results are reported as inhibition diameters (IDs) and minimum inhibitory concentrations (MICs), respectively. In order to investigate potential sources of variability in antimicrobial susceptibility testing results and to assess whether a correlation exists between ID and MIC measurements, meta-analytical regression models were built using in vitro data obtained through a systematic literature search. The pooled ID models revealed varied bacterial susceptibilities to the extracts and in some cases, the plant species and methodology utilised impacted the measurements obtained (p < 0.05). Lemon and orange extracts were found to be most effective against E. coli (24.4 ± 1.21 and 16.5 ± 0.84 mm, respectively), while oregano extracts exhibited the highest level of effectiveness against B. cereus (22.3 ± 1.73 mm). Clove extracts were observed to be most effective against B. cereus and demonstrated the general trend that the well-diffusion method tends to produce higher ID (20.5 ± 1.36 mm) than the disk-diffusion method (16.3 ± 1.40 mm). Although the plant species had an impact on MIC, there is no evidence to suggest that the methodology employed had an effect on MIC (p > 0.05). The ID–MIC model revealed an inverse correlation (R2 = 47.7%) and highlighted the fact that the extract dose highly modulated the relationship (p < 0.0001). The findings of this study encourage the use of extracts and EOs derived from Origanum, Syzygium aromaticum, and Citrus to prevent bacterial growth. Additionally, this study underscores several variables that can impact ID and MIC measurements and expose the correlation between the two types of results.
Comparative Study of Sensory and Physicochemical Characteristics of Green-Tea-Fortified Cupcakes upon Air Frying and Oven Baking
Authors: Ngan Hiu-Lok , Ip Shu-Yu , Wang Mingfu , Zhou Qian
Published: March 2023

The air fryer and the oven are common cooking methods in our daily lives. However, previous investigations of the air fryer were limited to its comparison with deep-fat frying. This study compared the differences between air frying and household oven baking (without a fan or other forced airflow inside) on food quality and physicochemical properties using a cupcake model. Results showed that the oven-baked cupcakes were softer in texture (87.15%), greener in color (6.07%), and lower in weight loss (7.78%) and toxic advanced glycation end products (AGEs, 21.40%) when the heating temperature and duration were the same as oven baking. To improve the sensory characteristics and health value, the cupcakes were fortified with green tea. The differences in texture, color, and level of toxicants between the two cooking methods were diminished after the addition of green tea. Moreover, the chemical profiles of green tea catechins in the green-tea-fortified cupcakes remained similar upon thermal cooking, except that the air-fried cupcakes were lower in gallic acid (GA) but higher in (−)-gallocatechin (GC). Collectively, based on the differences in heating mechanisms, our data indicated that oven baking is a better cooking method suitable to prepare cupcakes than air frying from the perspectives of sensory characteristics and food safety, while green tea additives effectively counter the drawbacks of the air fryer.
Effects of Extraction and Evaporation Methods on Physico-Chemical, Functional, and Nutritional Properties of Syrups from Barhi Dates (Phoenix dactylifera L.)
Authors: Julai Kanokporn , Sridonpai Pimnapanut , Ngampeerapong Chitraporn , Tongdonpo Karaked , Suttisansanee Uthaiwan , Kriengsinyos Wantanee , On-Nom Nattira , Tangsuphoom Nattapol
Published: March 2023

Date fruits (Phoenix dactylifera L.) are rich in sugar and also contain a substantial amount of phenolic compounds. Therefore, date fruits can be used to produce an alternative sweetener, having lower glycemic index than sucrose. This study investigated the effects of extraction and evaporation methods on various properties of the syrups prepared from Barhi dates. Extraction of date juice with the aid of pectinase or cellulase significantly enhanced the production yield, total phenolic content, and antioxidant activities determined by Ferric Reducing Antioxidant Power and Oxygen Radical Absorbance Capacity assays. Syrups prepared without enzyme application had about 6–7 times higher apparent viscosity than those prepared from the enzyme-assisted extracted juices. Vacuum evaporation produced syrups with significantly lighter color and inferior antioxidant properties than open heating. Properties of date syrups prepared with or without enzyme-assisted extraction followed by open heat evaporation were not different. They had a glucose-to-fructose ratio close to 1:1, received good sensory acceptability scores of above 6 on a 9-point hedonic scale, contained a safe level (<40 mg/kg) of 5-hydroxymethyl-2-furfuraldehyde, and exhibited similar glass transition and melting temperatures; while a greater inhibition on α-amylase activity was observed in syrups obtained from enzyme-assisted extraction. The in vivo glycemic measurement revealed that the syrup prepared with the aid of Pectinex and open heating was classified as low glycemic index (GI = 55) and medium glycemic load (GL = 11). Thus, enzyme-assisted extraction of date juice using Pectinex could be used to produce a healthy natural sweetener from low quality date fruits.
Involvement of Versatile Bacteria Belonging to the Genus Arthrobacter in Milk and Dairy Products
Authors: Sutthiwong Nuthathai , Lekavat Supaporn , Dufossé Laurent
Published: March 2023

Milk is naturally a rich source of many essential nutrients; therefore, it is quite a suitable medium for bacterial growth and serves as a reservoir for bacterial contamination. The genus Arthrobacter is a food-related bacterial group commonly present as a contaminant in milk and dairy products as primary and secondary microflora. Arthrobacter bacteria frequently demonstrate the nutritional versatility to degrade different compounds even in extreme environments. As a result of their metabolic diversity, Arthrobacter species have long been of interest to scientists for application in various industry and biotechnology sectors. In the dairy industry, strains from the Arthrobacter genus are part of the microflora of raw milk known as an indicator of hygiene quality. Although they cause spoilage, they are also regarded as important strains responsible for producing fermented milk products, especially cheeses. Several Arthrobacter spp. have reported their significance in the development of cheese color and flavor. Furthermore, based on the data obtained from previous studies about its thermostability, and thermoacidophilic and thermoresistant properties, the genus Arthrobacter promisingly provides advantages for use as a potential producer of β-galactosidases to fulfill commercial requirements as its enzymes allow dairy products to be treated under mild conditions. In light of these beneficial aspects derived from Arthrobacter spp. including pigmentation, flavor formation, and enzyme production, this bacterial genus is potentially important for the dairy industry.
Strategies for Exploiting Milk Protein Properties in Making Films and Coatings for Food Packaging: A Review
Authors: Gerna Stefano , D’Incecco Paolo , Limbo Sara , Sindaco Marta , Pellegrino Luisa
Published: March 2023

Biopolymers of different natures (carbohydrates, proteins, etc.) recovered from by-products of industrial processes are increasingly being studied to obtain biomaterials as alternatives to conventional plastics, thus contributing to the implementation of a circular economy. The food industry generates huge amounts of by-products and waste, including unsold food products that reach the end of their shelf life and are no longer usable in the food chain. Milk proteins can be easily separated from dairy waste and adapted into effective bio-based polymeric materials. Firstly, this review describes the relevant properties of milk proteins and the approaches to modifying them for subsequent use. Then, we provide an overview of recent studies on the development of films and coatings based on milk proteins and, where available, their applications in food packaging. Comparisons among published studies were made based on the formulation as well as production conditions and technologies. The role of different additives and modifiers tested for the performances of films and coatings, such as water vapor permeability, tensile strength, and elongation at break, were reviewed. This review also outlines the limitations of milk-protein-based materials, such as moisture sensitivity and brittleness. Overall, milk proteins hold great potential as a sustainable alternative to petroleum-based polymers. However, their use in food packaging materials at an industrial level remains problematic.
Discrimination and Characterization of Volatile Flavor Compounds in Fresh Oriental Melon after Forchlorfenuron Application Using Electronic Nose (E-Nose) and Headspace-Gas Chromatography-Ion Mobility Spectrometry (HS-GC-IMS)
Authors: Wang Qi , Chen Xueying , Zhang Chen , Li Xiaohui , Yue Ning , Shao Hua , Wang Jing , Jin Fen
Published: March 2023

Aroma is a crucial factor determining the market value and consumer satisfaction of fresh oriental melon. However, few studies focus on the volatile flavor of fresh oriental melon, and the effect of forchlorfenuron application on the aroma profile is unclear. This study characterized the volatile profile of fresh oriental melon fruit after forchlorfenuron application by E-nose and HS-GC-IMS. The holistic variation of volatile compounds exhibited evident distinction based on linear discriminant analysis (LDA) with E-nose. Forty-eight volatile compounds were identified from fresh oriental melon via GC-IMS, mainly esters, alcohols, aldehydes, and ketones, along with smaller quantities of sulfides and terpenes. Compared to pollination melon fruits, 13 critical different volatile flavor compounds were screened out in forchlorfenuron application groups by the PLS-DA model, imparting sweet fruity flavor. The results of the current study provide a valuable basis for evaluating the flavor quality of oriental melon after forchlorfenuron treatment.
Impact of Dilution on Whisky Aroma: A Sensory and Volatile Composition Analysis
Authors: Ashmore P. Layton , DuBois Aubrey , Tomasino Elizabeth , Harbertson James F. , Collins Thomas S.
Published: March 2023

An “omics”-style approach was used to evaluate the complex relationship between whisky aroma and dilution with water, typically suggested as a way to better appreciate whisky. A set of 25 samples, including Bourbons, ryes, single-malt and blended Scotches, and Irish whiskies were chemically profiled at six dilution levels (100, 90, 80, 70, 60, and 50% whisky/water), while a subset of six whiskies (three Bourbons, three Scotches) at four dilution levels (100, 80, 60, and 40% whisky/water) were chemically profiled and subjected to sensory analysis by a trained panel (n = 20). Untargeted volatile analysis was performed using headspace solid-phase microextraction gas chromatography coupled with mass spectrometry (HS-SPME-GC-MS) and sensory analysis was performed using descriptive analysis (DA). Results were evaluated using multivariate statistical techniques, including multifactor analysis (MFA) and partial least squares discriminant analysis (PLS-DA). Dilution decreased headspace concentration of hydrophilic aroma compounds and increased concentration of more hydrophobic compounds, which agreed with DA results. Dilution above 80% whisky/20% water reduced differences within whisky styles, though differences between American (Bourbon, rye) and Scotch styles (single malt, blended) continued to increase with further dilution. This provides important insight into how dilution of whisky during consumption changes consumer perception, as well as the usefulness of HS-SPME-GC-MS as a proxy for human olfaction.
Iturin A Strongly Inhibits the Growth and T-2 Toxin Synthesis of Fusarium oxysporum: A Morphological, Cellular, and Transcriptomics Study
Authors: Hua Meifang , Deng Qi , Qiu Mei , Deng Yijia , Sun Lijun , Fang Zhijia , Liao Jianmeng , Zhao Jian , Gooneratne Ravi
Published: March 2023

Fusarium oxysporum (F. oxysporum) is a common contaminant of dried fish, and the T-2 synthesis by this organism in dried fish products poses a serious public health risk. In this study, we investigated the effects of iturin A, a cyclic lipopeptide produced by Bacillus subtilis, on the growth and synthesis of the T-2 toxin of F. oxysporum, and transcriptomics was conducted. Results showed that the inhibitory effect of iturin A on F. oxysporum was significantly enhanced with an increase in iturin A concentrations. More specifically, compared with the control group, all indexes in the iturin A treatment group with 50 μg/mL were decreased to 24.84 mm, 0.33 × 106 cfu/mL, and 5.86 ng/mL for the colony diameter, number of spores, and concentration of T-2 toxin, respectively. Furthermore, iturin A was proven to destroy the integrity of cell membranes and cause a significant increase in ROS at 25 μg/mL or 50 μg/mL. Transcriptomic analysis revealed that with the treatment of iturin A, the genes of the oxidation-reduction process were up-regulated, while the gene expression of mycelial growth, cell integrity, transmembrane transport, energy metabolism, and others were down-regulated. More importantly, the Tri5 gene cluster was significantly inhibited. This study provided new insights into the mechanism for the inhibitory effect of iturin A on the growth and T-2 toxin synthesis of F. oxysporum and theoretical guidance for the application of iturin A in the preservation of dried aquatic products.
Understanding the Impact of High-Pressure Treatment on Physico-Chemical, Microstructural, and Microbiological Aspects of Pumpkin Cubes
Authors: Rinaldi Massimiliano , Dhenge Rohini , Rodolfi Margherita , Bertani Gaia , Bernini Valentina , Dall’Acqua Stefano , Ganino Tommaso
Published: March 2023

In this study color, texture, starch–pectin, total antioxidant capacity, microbial count, and microstructure of HPP-treated Violina pumpkin cubes were evaluated. Samples were treated at six different pressures (100 to 600 MPa–HPP100 to HPP600) for 3 min. Moisture, total soluble solids, and pH showed no significant differences between untreated (UNTR) and treated samples. Pumpkin tissue showed great structural modifications as changes in cell size and shape, cell wall damage, increased cell wall thickness, cell detachment and dehydration, and calcium ions deposition mainly from HPP300 to 600. UNTR samples showed the highest value of maximum and minimum cell elongation, perimeter segment, and a more regular cell wall thickness whereas HPP600 showed the lowest values for all these parameters. A noticeable difference was observed in HPP600 samples, with a difference in terms of color (ΔE 11.3 ± 1.9) and hardness (87.4 ± 27.8 N) compared to the UNTR ones (194.9 ± 37.9 N) whereas treatments at other pressures changed less markedly the color and texture. HPP200 could ensure a higher amount of starch and pectin availability while HPP200 and HPP400 showed the highest total antioxidants capacity. High-pressure treatment from HPP400 to 600 gave the highest destruction of microorganisms but negatively influenced the structural quality as well as texture and microstructure.
The Effect of Sage (Salvia sclarea) Essential Oil on the Physiochemical and Antioxidant Properties of Sodium Alginate and Casein-Based Composite Edible Films
Authors: Bhatia Saurabh , Al-Harrasi Ahmed , Shah Yasir Abbas , Jawad Muhammad , Al-Azri Mohammed Said , Ullah Sana , Anwer Md Khalid , Aldawsari Mohammed F. , Koca Esra , Aydemir Levent Yurdaer
Published: March 2023

The aim of this study was to examine the effect of Sage (Salvia sclarea) essential oil (SEO) on the physiochemical and antioxidant properties of sodium alginate (SA) and casein (CA) based films. Thermal, mechanical, optical, structural, chemical, crystalline, and barrier properties were examined using TGA, texture analyzer, colorimeter, SEM, FTIR, and XRD. Chemical compounds of the SEO were identified via GC–MS, the most important of which were linalyl acetate (43.32%) and linalool (28.51%). The results showed that incorporating SEO caused a significant decrease in tensile strength (1.022–0.140 Mpa), elongation at break (28.2–14.6%), moisture content (25.04–14.7%) and transparency (86.1–56.2%); however, WVP (0.427–0.667 × 10−12 g·cm/cm2·s·Pa) increased. SEM analysis showed that the incorporation of SEO increased the homogeneousness of films. TGA analysis showed that SEO-loaded films showed better thermal stability than others. FTIR analysis revealed the compatibility between the components of the films. Furthermore, increasing the concentration of SEO increased the antioxidant activity of the films. Thus, the present film shows a potential application in the food packaging industry.
Thermo-Responsive Injectable Hydrogels Formed by Self-Assembly of Alginate-Based Heterograft Copolymers
Authors: Safakas Konstantinos , Saravanou Sofia-Falia , Iatridi Zacharoula , Tsitsilianis Constantinos
Published: March 2023

Polysaccharide-based graft copolymers bearing thermo-responsive grafting chains, exhibiting LCST, have been designed to afford thermo-responsive injectable hydrogels. The good performance of the hydrogel requires control of the critical gelation temperature, Tgel. In the present article, we wish to show an alternative method to tune Tgel using an alginate-based thermo-responsive gelator bearing two kinds of grafting chains (heterograft copolymer topology) of P(NIPAM86-co-NtBAM14) random copolymers and pure PNIPAM, differing in their lower critical solution temperature (LCST) about 10 °C. Interestingly, the Tgel of the heterograft copolymer is controlled from the overall hydrophobic content, NtBAM, of both grafts, implying the formation of blended side chains in the crosslinked nanodomains of the formed network. Rheological investigation of the hydrogel showed excellent responsiveness to temperature and shear. Thus, a combination of shear-thinning and thermo-thickening effects provides the hydrogel with injectability and self-healing properties, making it a good candidate for biomedical applications.
Coronatine-Based Gene Expression Changes Impart Partial Resistance to Fall Armyworm (Spodoptera frugiperda) in Seedling Maize
Authors: Lou Yuxuan , Jin Xiaoxiao , Jia Zhiguo , Sun Yuqi , Xu Yiming , Liu Zihan , Tan Shuqian , Yi Fei , Duan Liusheng
Published: March 2023

In recent years, Spodoptera frugiperda (S. frugiperda, Smith) has invaded China, seriously threatening maize production. To explore an effective method to curb the further expansion of the harm of the S. frugiperda, this experiment used maize seedlings of the Zhengdan 958 three-leaf stage (V3) of maize as the material to study the effect of coronatine (COR) on the ability of maize to resist insects (S. frugiperda) at the seedling stage. The results showed that when maize was sprayed with 0.05 μM COR, the newly incubated larvae of S. frugiperda had the least leaf feeding. It was found that 0.05 μM COR significantly increased the contents of abscisic acid (ABA) and jasmonate (JA) in maize leaves through the determination of hormone content. Moreover, transcriptome sequencing revealed that the expression of six genes (ZmBX1, ZmBX2, ZmBX3, ZmBX4, ZmBX5 and ZmBX6), which are associated with the synthesis of benzoxazinoid, were upregulated. Nine genes (ZmZIM3, ZmZIM4, ZmZIM10, ZmZIM13, ZmZIM18, ZmZIM23, ZmZIM27, ZmZIM28 and ZmZIM38) of JA-Zim Domain (JAZ) protein in the JA signal pathway, and seven genes (ZmPRH19, ZmPRH18, Zm00001d024732, Zm00001d034109, Zm00001d026269, Zm00001d028574 and Zm00001d013220) of ABA downstream response protein Group A Type 2C Protein Phosphatase (PP2C) were downregulated. These results demonstrated that COR could induce anti-insect factors and significantly improve insect resistance in seedling maize, which laid a theoretical foundation for further study of the mechanism of COR improving insect resistance in seedling maize, and provided data references for the use of COR as an environmentally friendly pest control method.
The Expanding Phenotype of ZTTK Syndrome Due to the Heterozygous Variant of SON Gene Focusing on Liver Involvement: Patient Report and Literature Review
Authors: Pietrobattista Andrea , Della Volpe Luca , Francalanci Paola , Figà Talamanca Lorenzo , Monti Lidia , Lepri Francesca Romana , Basso Maria Sole , Liccardo Daniela , Della Corte Claudia , Mosca Antonella , Alterio Tommaso , Veraldi Silvio , Callea Francesco , Novelli Antonio , Maggiore Giuseppe
Published: March 2023

Zhu–Tokita–Takenouchi–Kim (ZTTK) syndrome, an intellectual disability syndrome first described in 2016, is caused by heterozygous loss-of-function variants in SON. Haploinsufficiency in SON may affect multiple genes, including those involved in the development and metabolism of multiple organs. Considering the broad spectrum of SON functions, it is to be expected that pathogenic variants in this gene can cause a wide spectrum of clinical symptoms. We present an additional ZTTK syndrome case due to a de novo heterozygous variant in the SON gene (c.5751_5754delAGTT). The clinical manifestations of our patient were similar to those present in previously reported cases; however, the diagnosis of ZTTK syndrome was delayed for a long time and was carried out during the diagnostic work-up of significant chronic liver disease (CLD). CLD has not yet been reported in any series; therefore, our report provides new information on this rare condition and suggests the expansion of the ZTTK syndrome phenotype, including possible liver involvement. Correspondingly, we recommend screening patients with SON variants specifically for liver involvement from the first years of life. Once the CLD has been diagnosed, an appropriate follow-up is mandatory, especially considering the role of SON as an emerging player in cancer development. Further studies are needed to investigate the role of SON haploinsufficiency as a downregulator of essential genes, thus potentially impairing the normal development and/or functions of multiple organs.
Threat Assessment and Risk Analysis (TARA) for Interoperable Medical Devices in the Operating Room Inspired by the Automotive Industry
Authors: Puder Andreas , Henle Jacqueline , Sax Eric
Published: March 2023

Prevailing trends in the automotive and medical device industry, such as life cycle overarching configurability, connectivity, and automation, require an adaption of development processes, especially regarding the security and safety thereof. The changing requirements imply that interfaces are more exposed to the outside world, making them more vulnerable to cyberattacks or data leaks. Consequently, not only do development processes need to be revised but also cybersecurity countermeasures and a focus on safety, as well as privacy, have become vital. While vehicles are especially exposed to cybersecurity and safety risks, the medical devices industry faces similar issues. In the automotive industry, proposals and draft regulations exist for security-related risk assessment processes. The medical device industry, which has less experience in these topics and is more heterogeneous, may benefit from drawing inspiration from these efforts. We examined and compared current standards, processes, and methods in both the automotive and medical industries. Based on the requirements regarding safety and security for risk analysis in the medical device industry, we propose the adoption of methods already established in the automotive industry. Furthermore, we present an example based on an interoperable Operating Room table (OR table).
Effect of Counseling Service on Breastfeeding Practice among Saudi Mothers
Authors: AlQurashi Alaa , Wani Tariq , Alateeq Nouf , Heena Humariya
Published: March 2023

Objective: To assess the knowledge and practice of breastfeeding and the effects of lactation counseling, as a health promotion intervention, on breastfeeding duration and exclusive breastfeeding (EBF) among Saudi women. Methods: In this quasi-experimental design study, 664 mothers attending a women’s hospital from January 2017 to December 2018 were interviewed. Women were allocated into two groups, control and intervention groups, based on received lactation counseling. Interviews were performed using a structured questionnaire collecting data on the sociodemographic characteristics, knowledge, attitude and practice of breastfeeding mothers. A chi-squared test was used to determine the level of significance on breastfeeding practices among two groups. Propensity score matching was presented to control confounders, as women cannot be randomly assigned to lactation counseling. Results: Of 664 women, 592 were Saudi nationals, and the majority of mothers were literate (96.2%). A significantly higher number of mothers in the consultation group were employed as compared with the non-consultation group (p = 0.015). One third (33.3%) of the women practiced EBF, while 39.8% preferred mixed feeding for the first six months of the infant’s life. The consultation group demonstrated a significantly higher response rate in their knowledge on the benefits of breastfeeding in babies (increased intelligence; p < 0.05) and mothers (breast engorgement; p = 0.004), colostrum and its importance (p = 0.027) and effective breastfeeding practices (initiate breastfeeding within 30 min after birth (p = 0.01), baby needs 10–20 min between each feed (p = 0.009), breastfeeding should last for 6 months (p = 0.01)) compared with the non-consultation group. The age of weaning (5.3 ± 2.8 vs. 5.9 ± 3.2 months) was similar across both the groups. However, “the intended duration of BF” was higher in the non-consultation group, and the difference was statistically significant (p = 0.002). The mean weight and length of the baby at follow-up were similar in both groups, with no statistical significance. The duration of exclusive breastfeeding among two groups was not statistically significantly different (8.7 ± 6.9 vs. 8.1 ± 7.1 weeks). Mothers in the two groups were satisfied with their breastfeeding experience. The mean scores lie within the range of 4.2 to 5.0. Baby age (month), mother LoE, mother job and type of BF were controlled for, and the propensity-score-matched 62.5% sample from both the groups yielded the same results. Conclusions: Breastfeeding women in our study showed a fair knowledge of EBF. However, the duration of actual EBF was very short, and the counseling intervention showed no impact on EBF in our study population. We recommend interventions that are tailored to the needs of this population, while identifying factors that improve breastfeeding practice among mothers.
Sexual Desire and Erotic Fantasies Questionnaire: Validation of the Erotic Fantasy Inventory Scale (SDEF3) in Italian Adults
Authors: Nimbi Filippo Maria , Galizia Roberta , Fontanesi Lilybeth , Soyman Seray , Jannini Emmanuele Angelo , Simonelli Chiara , Tambelli Renata
Published: March 2023

Background: Erotic fantasies are the most common sexual experiences and provide valuable clinical material for understanding individual and relational emotional dynamics. The primary objective of this study is to validate the Sexual Desire and Erotic Fantasies questionnaire (SDEF) Part 3–Inventory of Erotic Fantasies. This questionnaire was designed to be a sex-positive and inclusive measure of the content of erotic fantasies, accessible to individuals of all gender identities, sexual orientations, relationship/romantic status, and sexual behaviors. Methods: The SDEF3 was completed by 1773 Italian participants (1105 women, 645 men, and 23 participants identifying as other genders). Two factorial structures were presented and discussed: a 20-dimension structure for clinical and explorative use and a 6-dimension structure for research purposes. Results: The six-factor version was preferred due to its robust statistical properties and its ability to differentiate between sexually clinical and functional men and women, based on cut-off scores from the Female Sexual Function Index (FSFI) and the International Index of Erectile Function (IIEF). Differences in the frequency of themes in fantasies between gender and sexual orientation were reported and discussed. Conclusions: The current study indicates that the SDEF3 is a valuable and comprehensive measure for assessing various scenarios related to fantasizing activity. It has potential applications in both clinical practice and scientific research.