bluuebunny/crossref_metadata_2025 huggingface dataset:
Consumer Protection Law: The Case Study Of Grabtoko Company In Indonesian E-Commerce Transactions
Authors: Tjipto Felix Pratama , Ong Jason Pratama , Sulistio Marshal Ramadhan , Harnowo Tri
Published: August 2022

ABSTRACTTechnology innovation is steadily increasing its capacity in helping human activities, one of which is through digital transactions. The increased use of technology, however, could potentially create legal loopholes which may violate consumer’s rights in online transactions like the recently happened GrabToko case. This journal shall discuss and provide normative approach analysis in Indonesian customer protection law, with the reference of previous articles, seeing the increasing usage of digital transactions. This journal aims to provide an explanationregarding the advantages and the disadvantages of existing regulations and what needs to be improved related to frauds in the case of GrabToko. Furthermore, it will also be discussed about the legal responsibility of the consumer dispute settlement organizations. The results of this paper shows that there are still needs of improvements since the system has its own advantages and disadvantages, especially regarding fraud and administration systems that need to be updated in corresponding law and the development in the aspect consumer protection law.Keywords: consumer protection law; consumer; rights; transaction; regulation; consumer dispute settlementINTISARIInovasi teknologi terus meningkatkan kemampuannya dalam membantu aktivitas manusia, salah satunya melalui transaksi digital. Namun, peningkatan penggunaan teknologi berpotensi menciptakan celah hukum yang berpotensi melanggar hak konsumen dalam bertransaksi online seperti kasus GrabToko yang baru-baru ini terjadi. Jurnal ini akan membahas dan memberikan analisis pendekatan normatif dalam hukum perlindungan nasabah Indonesia, dengan mengacu pada artikel sebelumnya, melihat penggunaan transaksi digital yang semakin meningkat. Jurnal ini bertujuan untuk memberikan penjelasan mengenai kelebihan dan kekurangan regulasi yang ada serta apa saja yang perlu ditingkatkan terkait fraud pada kasus GrabToko. Selanjutnya juga akan dibahas tentang tanggung jawab hukum dari organisasi penyelesaian sengketa konsumen. Hasil dari makalah ini menunjukkan bahwa masih terdapat kebutuhan perbaikan karena sistem tersebut memiliki kelebihan dan kekurangan, terutama terkait dengan sistem penipuan dan administrasi yang perlu diperbarui sesuai peraturan perundang-undangan dan perkembangan dalam aspek hukum perlindungan konsumen.Kata Kunci: hukum perlindungan konsumen; konsumen; hak; transaksi; regulasi; penyelesaian sengketa konsumen.
Acuerdos de solución amistosa bajo la Comisión Interamericana de Derechos Humanos (Periodo 2010-2020): Un análisis de las medidas de reparación acordadas y su grado de cumplimiento
Authors: Otero Rodríguez R. Adelayda
Published: August 2022

Friendly Settlement Agreements are a tool through which petitions can be resolved in a non-contentious manner and agreed upon by the parties. There are several types of reparation that are adopted in friendly settlement agreements and these are: medical, psychological and social rehabilitation, measures of satisfaction, economic compensation, as well as measures of non-repetition. This research work is based on the content analysis method; in reference to the description of the data analysis, the reading of 100 cases of friendly settlement from 2010 to 2020 was taken as a basis, the investigative approach is mixed and the design implemented is the case study. The main objective is to analyze the degree of total, partial, substantial partial and pending compliance of the friendly settlement agreements approved by the Inter-AmericanCommission on Human Rights during the period 2010 to 2020. The results of the investigation yielded a significant fact that most the ASA reports are at the partial compliance level, with a high number of 53 cases out of the 100 sampled. To conclude, it is essential to emphasize that the procedure is also an opportunity for States to demonstrate their level of commitment to respect human rights and their duty to ensure their protection and compliance. In addition, it prevents a sociopolitical impact that causes a conviction against States and saves important resources.
ІННОВАЦІЙНО-ТЕХНОЛОГІЧНИЙ РОЗВИТОК ЕКОНОМІКИ: СТРАТЕГІЧНІ ІМПЕРАТИВИ РЕАЛІЗАЦІЇ ПОТЕНЦІАЛУ РЕГІОНАЛЬНОГО РОЗВИТКУ
Authors: Куницька-Іляш М. , Гримак О. , Дубина М. , Березівський Я. , Збарська А.
Published: August 2022

Вступ. Якісна ідентифікація рівня розвитку інноваційно-технологічної діяльності в країні та її міжрегіональних диспропорцій потребує формування більш досконалого інформаційно-аналітичного забезпечення державної політики.Проблематика. Актуальним на сьогодні є вивчення проблеми регіональних диспропорцій інноваційно-технологічного розвитку національної економіки України, пошуку чинників їх формування, а також визначення стратегічних імперативів, інструментів і засобів державної політики активізації інноваційно-технологічної діяльності в системі політики регіонального розвитку.Мета. Наукове обґрунтування інноваційно-технологічних аспектів розвитку економіки та виділення стратегічних імперативів забезпечення та вирівнювання міжрегіональних диспропорцій.Матеріали й методи. Як методичну основу дослідження використано положення кореляційно-регресійного, системно-структурного та статистичного аналізу й групування, теорій економічного зростання, сучасних концепції перехідних економічних систем.Результати. Розроблено та апробовано авторську методику аналізування рівня інноваційно-технологічного розвитку та ідентифікації міжрегіональних диспропорцій інноваційно-технологічного зростання національної економіки. На основі інтегральних значень інноваційно-технологічного зростання регіонів виявлено та пояснено характер міжрегіональних диспропорцій в аналізованій сфері. Ідентифіковано 6 груп регіонів України відповідно до значень інтегрального індексу інноваційно-технологічного розвитку регіональних економік. Визначено стратегічні імперативи подальшого інноваційно-технологічного розвитку для кожної з груп.Висновки. Викладені в дослідженні науково-методичні засади та практичні рекомендації спрямовано на підвищення ефективності управління національною економікою, забезпечення її стійкої життєздатності та реалізації потенціалу в системі міжнародного поділу праці.
РОЗРОБКА КОНСТРУКТИВНОЇ СХЕМИ ТА ВИБІР ПАРАМЕТРІВ АЕРОДИНАМІЧНОЇ СИСТЕМИ ВІДВЕДЕННЯ КОСМІЧНИХ АПАРАТІВ РОЗРОБКИ ДП КБ «ПІВДЕННЕ» З ОРБІТИ
Authors: Алпатов А. , Лапханов Е. , Палій О.
Published: August 2022

Вступ. Для стабілізації середовища космічного сміття відпрацьовані космічні апарати та верхні ступені ракет-носіїв необхідно відводити з орбіти.Проблематика. Проведений аналіз надувних аеродинамічних систем відведення космічних апаратів з орбіти показав, що вони є ефективним засобом відведення космічних апаратів з орбіти на висотах до 800 км, однак мають певні недоліки: ймовірність пошкодження фрагментами космічного сміття через чутливість матеріалу оболонки, а також ймовірність електростатичного пробою.Мета. Розробка конструктивної схеми та вибір параметрів аеродинамічної системи відведення космічних апаратів, розроблених ДП «КБ «Південне», з орбіти.Матеріали й методи. Методи механіки космічного польоту, математичне моделювання задач проєктування.Результати. Розрахунки показали, що час відведення космічного апарату «Січ-2-1» із планованої орбіти складає близько 6,5 років при масі аеродинамічної системи відведення 9 кг, що складає 5% від маси зазначеного космічного апарата. Визначено, що у разі збільшення часу відведення космічного апарата «Січ-2-1» з планованої орбіти після завершення експлуатації до 25 років, масу аеродинамічної системи можна зменшити до 4,5 кг. При масі аеродинамічної системи відведення в 9 кг, межею ефективного застосування зазначеної аеродинамічної системи відведення є висоти від 730 до 750 км на близьких до кругових орбітах різної дислокації і висоти не більше 700 км в перигеї та 842 км в апогеї на малоеліптичних орбітах.Висновки. Виходячи із вимог ДП КБ «Південне» до масових і габаритних параметрів засобу відведення, було розроблено конструктивну схему і проєктний вигляд аеродинамічної системи відведення, що розгортається. Особливістю конструкції є компактність, що забезпечується застосуванням пружинних механізмів і маловитратних мікроелектродвигунів, що розгортають аеродинамічні елементи. Така конструкція займає незначний об’єм на космічному апараті «Січ-2-1»
Innovative System of Computer Modelling of Multiphysics Processes for Controlled Electrocurrent Treatment of Melts
Authors: Zaporozhets Yu. , Ivanov A. , Kondratenko Yu. , Tsurkin V. , Batechko N.
Published: August 2022

Introduction. The widespread use of cast products made of aluminum and its alloys requires ensuring a highquality structure of the castings, on which their operational properties depend. Controlling the process of forming a high-quality structure of castings is possible, in particular, by the method of electrocurrent treatment of melts.Problem Statement. The melt medium being inaccessible for direct measurement of the processing parameters, the only way to realize the control of treatment conditions is numerical simulation of these parameters. However, the complexity and interdependence of multiphysics processes of melt electrocurrent treatment have led to an unconventional approach to the formulation of their mathematical models and computational procedures. These circumstances have determined the features of the tasks for the construction of appropriate computer models and their application.Purpose. The development of a new pattern-modular system for computer modeling of multiphysics processes of electric current treatment of melts to control the conditions of the formation of a qualitative structure of castings.Materials and Methods. The material of the research is a set of model problems of multiphysical processes of electrocurrent treatment and their ontology, the integral equations and their properties, as well as databases on the parameters of simulated objects, which describe these processes. The method of ontological taxonomy has been used to create a taxonomic codifier, with the help of which model problems and mathematical tools for their solution have been systematized. Based on the signs of similarity, the method of formalization of integral equations of coupled multiphysics processes has been applied.
Results. The unified patterns of basic algorithmic procedures and a library of program modules for computing operations of partial tasks, for which a unique code is assigned according to the codifier have been developed. Combining the patterns with different modules that are identified by the indicated codes has made it possible to form a wide range of computer models reflecting multiphysics processes. A flexible system for computer modeling of multiphysical processes has been built and its efficiency for simulating the modes of electrocurrent treatment of melts has been confirmed.Conclusions. The results obtained have enabled controlling the conditions of electrocurrent treatment of melts to form a highquality structure of cast metal.
Contra a “avalanche dêsse género de literatura importada e de grande penetração popular”
Authors: Gomes Ivan Lima
Published: August 2022

A publicação de histórias em quadrinhos no Brasil consolidou-se durante os anos 1940 e 1950 a partir de editoras voltadas à produção e distribuição de revistas em quadrinhos. Uma das consequências desse cenário foi a manifestação de vozes críticas à presença dessa forma de expressão no Brasil. Mídia associada, então, aos Estados Unidos, os quadrinhos eram acusados de serem uma expressão alheia à cultura brasileira. Na esteira destas restrições críticas, seguiram-se reivindicações de artistas brasileiros que passaram a defender espaço no mercado de quadrinhos para criadores locais, exigindo que fossem valorizados temas e personagens associados à cultura popular e à história do Brasil. Tais movimentos se articularam em torno de associações de desenhistas, que avançaram na crítica ao imperialismo dos Estados Unidos e fizeram ecoar suas demandas junto a setores da imprensa e da classe política. O artigo descreve essa história, analisando a formação de associações de desenhistas nos estados do Rio de Janeiro, São Paulo e Rio Grande do Sul, suas repercussões no jornal Última Hora e o que consideramos como ápice deste movimento: a constituição de uma cooperativa de artistas de quadrinhos em Porto Alegre, entre 1961 e 1964 – a Cooperativa Editora e de Trabalho de Porto Alegre (CETPA). Analisamos essa história como um momento importante para a constituição – não sem percalços e tensões – de uma identidade cultural brasileira sobre os quadrinhos, inspirada, em grande medida, no estabelecimento de um contraponto crítico em relação aos comics dos Estados Unidos.
Knowledge, Attitudes and Practices Related to Oral Health in First-Year University Students of the Paraíso Campus of the University of Costa Rica
Authors: Barahona-Cubillo Juan Bautista , Rojas-Brenes Cinthya , Barboza-Solís Cristina
Published: August 2022

People’s knowledge, attitudes, and practices related to health are built throughout life, particularly during childhood and adolescence, and they reflect the practices of their family and social circle. The aim of this study was to identify the levels of knowledge, attitudes, and practices related to oral health of first-year university students at the Paraíso Campus of the University of Costa Rica. This was done through a questionnaire of closed questions that would allow oral health educational strategies to be established in the future for this population group. For this purpose, a cross-sectional descriptive study was conducted, where 70 first-year students of the Paraíso Campus participated in the first semester of the year 2019.  For data collection, a survey of 22 closed questions on knowledge, attitudes, and practices about oral health was applied, and a score on oral health knowledge was subsequently performed, based on the answers to the questions. According to the results, the level of knowledge shown about oral health is low, since the average knowledge score (7.02 points) was less than half of the maximum score (17 points). The greatest deficiency in knowledge was evidenced in topics, such as, dental biofilm, gingivitis, use of fluorides, and the role of saliva. In addition, several practices that affect the condition of the oral cavity were identified, such as harmful habits, inadequate nutrition, and poor oral hygiene habits. In this study, a general lack of knowledge was identified regarding basic concepts of oral health in young university students, who are not clear about the role of some protective or risk factors related to the main oral diseases.
Altazor y Trilce: rupturas historiográficas en la vanguardia literaria latinoamericana
Authors: Mora Diego
Published: August 2022

El presente trabajo analiza los elementos vanguardistas en la obra de César Vallejo y Vicente Huidobro, a partir de los contrastes estéticos y autobiográficos de sus producciones literarias, específicamente en Trilce (1922) y Altazor (1931), libros que marcan un quiebre radical en la historiografía de la literatura latinoamericana y una doble escisión en sus autores: la personal y la histórica.
Bringing Religious Moderation Insights to “TikTok Da’wah”
Authors: Ahmad Fairuz Zakiyah , Emeraldien Fikry Zahria , Permatasari Rahayu Dia , Zahda Vizya Hanna
Published: August 2022

Preventing the spread of religious radicalism has remained popular issue in Indonesian nowadays. This is because Indonesia has long been vulnerable to Islamic radicalism ideologies. Many studies have revealed that young people have become the main target of the spread of Islamic extremist movement. This paper offers a textual analysis of ‘TikTok da’wah’ utilised by Muslim preachers to convey religious moderation teachings for young generation. In other words, it examines the use of Tiktok social media as a forum for mediating da'wah messages regarding religious moderation. The study results indicate that TikTok is an essential communication media for mubaligh to carry out da'wah for the millennial generation. In the hands of Islamic preachers, TikTok is likely to become a medium to build religious moderation insights amongs Indonesian youth.
Da'wah bi al-Hal within Islamic-Based Philanthropy: A Case Study of Darul Muhajirin Orphanage in Semarang
Authors: Qomariyah
Published: August 2022

This article attempts to discuss how the Islamic philanthropy of Darul Muhajirin orphanage in Semarang conducts da’wah bi al-hal (preaching by actual actions) towards the Muslim community living surrounding the orphanage and orphan children. Using a descriptive qualitative approach, the findings point out that da’wah bi al-hal conducted by Darul Muhajirin orphanage for community development and empowerment is an attempt to take part in community development efforts through social service activity programs. Hence, the existence of Darul Muhajirin orphanage is considered a benefit to the surrounding community.  In regard to the method of da’wah bi al-hal, Darul Muhajirin orphanage has proposed several ways: first, exemplary in social movements like charity. Second, exemplary in worship; third, exemplary in the manner of speaking.
Islam Nusantara dan Diskursus Politik: Analisis Wacana Kritis Berita di CNN Indonesia
Authors: Mahmud Hibatul Wafi  , Saipudin Ikhwan  , Tito Handoko
Published: August 2022

This paper reports on textual analysis research of three news media stories published by CNN Indonesia (cnninodenesia.com) in 2019 pertaining to Islam Nusantara and politics, using a critical discourse analysis approach proposed by Teun A. Van Dijk. Findings reveal that in the political context of Islam Nusantara (Islam of the Archipelago) discourse offers solutions to prevent conflict, tragedy, and violence in the name of religion. Further, it is also found that the political situation in Indonesia, which was rife with religious issues and increased intolerance, made Islam Nusantara dragged into the political sphere. This is because Partai Kebangkitan Bangsa (The National Awakening Party/PKB) which was historically born from the womb of the Nahdlatul Ulama was one of the ruling coalition parties.
Effect of planting methods on growth, yield, quality and economics of maize (Zea mays L.)
Authors: Kaur Amandeep , Kaur Ramandeep
Published: August 2022

The optimum time for sowing of Kharif maize in northern India is from last week of May to end of June. This period is characterized by a high evaporative demand due to high temperature and low relative humidity. The planting of maize during Kharif season experiences high rainfall in monsoon season which often causes temporary flooding in flat method of sowing. Planting methods in maize play a prominent role for reducing its water requirement when sown during Kharif season and also to save it from temporary flooding damage due to excessive rainfall during monsoon period. Excess water during the heavy rains especially on poorly drained soils also creates a great problem for maize cultivation as the crop is quite sensitive towards water stagnation. Best method of sowing is necessary to obtain maximum yield and higher net returns from maize cultivation. The findings of the various research scientists and eminent investigators will help in understanding effect of planting methods on growth, yield parameters, yield, quality and economics of maize. Among different planting methods, bed planting is found significant better in improving the growth, yield attributes, yield (grain and stover yield), quality and net returns in Kharif maize as compared to flat sowing method and it is followed by ridge sowing method. Bed and ridge planting methods give higher grain yield than the flat sowing method due to better crop growth and nutrient absorption. Water use efficiency and nutrient uptake was higher in bed planting as compared to flat and ridge planting methods.
Mine tailings-based geopolymers: Physical and mechanical properties
Authors: Akeed Mahmoud H.
Published: August 2022

The mining sector generates a substantial quantity of stone waste and tailings, which constitutes an environmental risk. The most prevalent method for disposing of this industrial waste is dumping, which contributes to soil deterioration and water contamination while acquiring precious land. It can be recycled using a number of processes, such as the promising geopolymerization technique, which transforms waste into value. This study reviews current developments in the manufacturing of mine tailings-based geopolymer composites from industrial waste as a possible sustainable building material. This paper also gives in-depth studies on the characteristics and behaviors of mine tailings composites used in geopolymer manufacturing, including physical and mechanical properties. This review also identifies knowledge gaps that must be filled in order to advance mine tailings composites for geopolymers.
PA2G4P4 Promotes Glioma Cell Migration and Tumorigenesis through the PTEN/AKT/mTOR Signaling Pathway
Authors: Hou Xiaofeng , Kou ZhengXiong , Zhang Hengzhu
Published: August 2022

Dysregulation of pseudogene expression is closely related to the progression of various cancers, including glioma. Proliferation-associated 2G4 pseudogene 4 (PA2G4P4) could affect cell viability and apoptosis of glioma cells. However, the specific regulatory mechanism of PA2G4P4 is not clear. In this paper, we found that PA2G4P4 overexpres-sion promoted glioma cell proliferation, migration and cell cycle progression, whereas PA2G4P4 knockdown inhibited cancer progression. Knockdown of PA2G4P4 also suppressed the tumorigenesis of glioma cells in vivo. Furthermore, knockdown of PA2G4 after overexpression of PA2G4P4 decreased the cell viability and migration ability to normal level. The protein level of a tumor suppressor gene phosphatase and tensing homolog (PTEN) was greatly decreased in U87 cells after PA2G4P4 overexpression, while increased after PA2G4 knockdown; on the contrary, the protein levels of P-AKT and P-S6 were obviously induced in U87 cells after PA2G4P4 overexpression, and decreased after PA2G4 knockdown. The cell ability, colony formation ability and cell migration ability were all recovered to normal level by adding an AKT inhibitor MK2206 to the glioma cells, which were induced by PA2G4P4 overexpression. Our results revealed that PA2G4P4 could regulate glioma cell proliferation and migration through PTEN/AKT/mTOR signaling pathway by targeting PA2G4 gene. PA2G4P4 may become a target for glioma treatment.
Paving block mortars made with mixed waste glass from municipality
Authors: Yomthong Krissanapat , Wattanasiriwech Suthee , Wattanasiriwech Darunee
Published: August 2022

To reduce the negative environmental impact of waste glass, this research study aims to incorporate mixed types of municipal waste glass in cement block mortars. The waste glass was first crushed, milled and screened with 18 and 20 meshed sieves to obtain the waste glass powder (WGP). The WGP, grouped into three sizes – small enough to pass through (P20WGP; 95% finer than 125 μm) and too large to pass through a 20 mesh sieve (T20WGP), as well as an equal mix of both sizes (M20WGP) – was used to replace sand in the range 0–100% in the cement block mortar. The specific strength of mortars was enhanced by increasing the WGP content. X-ray diffraction analysis suggested that carbonation at the early age observed in the P20WGP mortars could accelerate strength development. The extended working time test showed that the fresh WGP mortars had the potential for casting in the first 120 min. Different amounts of WGP used in mortars compacted by hand did not produce a significant difference in mechanical properties, suggesting that WGP could replace sand at all percentages. The surface temperature change, as a function of solar irradiated time, of mortar blocks compacted both by hand and hydraulically was similar.
Phloretin Improves Ultrafiltration and Reduces Glucose Absorption during Peritoneal Dialysis in Rats
Authors: Bergling Karin , Martus Giedre , Öberg Carl M.
Published: August 2022

Significance Statement

In peritoneal dialysis, ultrafiltration is achieved at the cost of systemic glucose absorption with succeeding treatment-induced metabolic side effects and comorbidities. The transport pathway mechanisms that enable glucose transport and the extent to which they individually contribute to mass transfer are not fully understood. Intraperitoneal administration of phloretin, a nonselective blocker of facilitative glucose transporters (GLUTs), reduced glucose absorption and improved ultrafiltration during peritoneal dialysis in rats. Because GLUTs are also present in the human peritoneal membrane, phloretin or similar acting agents could reduce the metabolic cost of ultrafiltration and improve outcomes of peritoneal dialysis.

Background

Harmful glucose exposure and absorption remain major limitations of peritoneal dialysis (PD). We previously showed that inhibition of sodium glucose cotransporter 2 did not affect glucose transport during PD in rats. However, more recently, we found that phlorizin, a dual blocker of sodium glucose cotransporters 1 and 2, reduces glucose diffusion in PD. Therefore, either inhibiting sodium glucose cotransporter 1 or blocking facilitative glucose channels by phlorizin metabolite phloretin would reduce glucose transport in PD.

Methods

We tested a selective blocker of sodium glucose cotransporter 1, mizagliflozin, as well as phloretin, a nonselective blocker of facilitative glucose channels, in an anesthetized Sprague–Dawley rat model of PD.

Results

Intraperitoneal phloretin treatment reduced glucose absorption by >30% and resulted in a >50% higher ultrafiltration rate compared with control animals. Sodium removal and sodium clearances were similarly improved, whereas the amount of ultrafiltration per millimole of sodium removed did not differ. Mizagliflozin did not influence glucose transport or osmotic water transport.

Conclusions

Taken together, our results and previous results indicate that blockers of facilitative glucose channels may be a promising target for reducing glucose absorption and improving ultrafiltration efficiency in PD.
Digital Education Challenges and Opportunities
Authors: Rani Geeta , Kaur Puninder , Sharma Taruna
Published: August 2022

Abstract : Online education is existing since 2014 but it becomes the only mode of instruction in 2020 due to the Covid pandemic. The whole world faces a lot of challenges, especially in developing countries. It is because in developing countries technology is confined to metropolitan cities only. A major portion of these countries are still rural and cannot provide the necessary infrastructure for digital education. Even aft er these chal lenges, it brings manifold opportunities too. This paper will study the opportunities and challenges faced while providing education in Online Mode from the perspective of s t u d e n t s , t e a c h e r s , a n d e d u c a t i o n Institutes/Universities. Keywords : Digital Education, Online education, Co v i d - 1 9 , Ch a l l e n g e s , Op p o r t u n i t i e s , Recommendations
Oral rehabilitation of a patient with Sjögren syndrome
Authors: Merima Balić , Zoltan Ovari
Published: August 2022

Sjögren syndrome (SS) is a chronic autoimmune disease characterized by inflammation of salivary and lacrymal glands, causing oral and ocular dryness [1]. The syndrome was named by Swedish ophthalmologist Henrik Sjögren, who identified it in 1933. SS prevalence ranges between 0. 5 % and 3 % and the syndrome is more likely to affect females over 40 years old, especially in menopause [2, 3]. It is a slowly progressing, non-life-threatening disease with a 10-year cumulative survival rate of over 90 % [4].
Using the Thermo SPD1010 speedvac concentrator centrifuge for drying down peptides for LC-MS analysis v1
Authors: not provided ronan.ocualain
Published: August 2022

This protocol details the procedure of using the Thermo SPD1010 speed vacuum concentrator centrifuge to dry peptide samples for storage and submission for LC-MS analysis. Peptides may be stored at or when dried down this way.
Protocol to secretome investigation of tumor 3D co-culture model v2
Authors: MARIA AP FERNANDES ANNA
Published: August 2022

Three-dimensional (3D) cell culture technologies, which more closely mimic the complex microenvironment of tissue, are being increasingly evaluated as a tool for the preclinical screening of clinically promising new molecules, and for the study of tissue metabolism. Studies of metabolites released into the extracellular space (secretome) allow understanding the metabolic dynamics of tissues and changes caused by therapeutic interventions. Although quite advanced in the field of proteomics, studies on the secretome of low molecular weight metabolites (< 1500 Da) are still very scarce. We present an untargeted metabolomic protocol based on the hybrid technique of high-performance liquid- chromatography coupled with high-resolution mass spectrometry for the analysis of low-molecular-weight metabolites released into the culture medium by 3D cultures and coculture (secretoma model). For that, we analayzed HT-29 human colon carcinoma cells and 3T3-L1 preadipocytes in 3D-monoculture and 3D-coculture. This protocol represents a possibility to list metabolites released in the extracellular environment in a comprehensive and untargeted manner, opening the way for the generation of metabolic hypotheses that will certainly contribute to the understanding of tissue metabolism, tissue-tissue interactions, and metabolic responses to the most varied interventions. Moreover, it brings potential to determine novel pathways and identify accurate biomarkers in cancer and other diseases. The metabolites indicated in our study have a close relationship with the tumor microenvironment in accordance with the literature review. For the 3D cell culture by levitation we used Bio-Assembler™ system (Bio Science 662840, Greiner One Bio, Americana, Brazil) in the n3D Biosciences and adapted protocol published by: HAISLER, W. L. et al. Three-dimensional cell culturing by magnetic levitation. Nature protocols, v. 8, n. 10, p. 1940-1949, 2013. https://doi.org/10.1038/nprot.2013.125
Una guerra sin monumentos: Rebelión de los oficios inútiles de Daniel Ferreira
Authors: Gonzalez Echeverry Angela
Published: August 2022

Este artículo analiza el sentido de inutilidad en la novela Rebelión de los oficios inútiles (2014) del autor colombiano Daniel Ferreira haciendo uso de la teoría marginalista de William S. Jevons y sus postulados económicos. El estudio sugiere líneas interpretativas trasversales a partir del relato literario de una humanidad deshecha por un orden socioeconómico sostenido por la premisa valor-igual utilidad. El aporte de esta lectura, es entre tanto trazar ecos entre los hechos recientes y el discurso cultural generalizado que criminaliza la pobreza y justifica la eliminación de quienes se consideran sujetos humanos “inútiles” dejando a su paso de esta manera la experiencia de quienes murieron en una guerra continua sin monumentos.
Estela del azar / Wake of Chance de Consuelo Hernández
Authors: Fuentes Víctor
Published: August 2022

Reseña de Estela del azar de Consuelo Hernández
No give up, maan!, de Hazel Robinson, desde la periferia de la comunidad letrada
Authors: Pérez Hernández Nayra , Becerra Bolaños Antonio
Published: August 2022

No give up, maan! ¡No te rindas! (2002), de Hazel Robinson Abrahams (1935), puede considerarse como una ficción (re)fundacional. Desde una postura decolonial, que visibiliza un territorio y una historia no contados, esta novela pone sobre la mesa la imposibilidad de una nación colombiana única y homogénea, al tiempo que rompe el canon literario nacional, especialmente desde el tratamiento del creole, y termina reescribiendo esta ultraperiférica región, rompiendo mitos sobre la identidad raizal al poner el acento sobre las huellas de africanía en que esta se fundaría.
Aplicaciones clínicas de la ecuación del movimiento del sistema respiratorio para la toma de decisiones en el paciente bajo ventilación mecánica invasiva: artículo de reflexión
Authors: Pérez-Nieto Orlando Rubén , Mancilla-Galindo Javier , Mendiola-Villalobos Carlos , Carrión-Moya Jorge Daniel , Zamarrón-López Eder Iván , Guerrero-Gutiérrez Manuel Alberto , Deloya-Tomás Ernesto , Sánchez-Díaz Jesús Salvador , Escarraman-Martinez Diego , Vásquez-Cuéllar Adolfo Israel , Kammar-García Ashuin , Soriano-Orozco Raúl , Castañón-González Jorge Alberto , Gordo-Vidal Federico
Published: August 2022

Introducción: la ventilación mecánica es una práctica común en las unidades de cuidados intensivos y anestesiología. Tiene implicaciones terapéuticas, pero también es potencialmente nociva para el sistema respiratorio y los órganos distantes, por lo cual es imprescindible monitorizar los parámetros ventilatorios de manera continua.

Objetivo: describir la ecuación del movimiento del sistema respiratorio y sus aplicaciones clínicas en el paciente bajo ventilación mecánica.

Desarrollo: la ecuación del movimiento del sistema respiratorio integra las fuerzas dinámicas generadas por el ventilador y las propiedades intrínsecas del pulmón y la caja torácica. Expresa la presión en el sistema respiratorio en relación con el volumen, la elastancia, la resistencia, el flujo de aire y las presiones generadas por el ventilador y el paciente. Las presiones elevadas en el sistema respiratorio se asocian a una mayor mortalidad en pacientes con ventilación mecánica, por lo que la identificación de los componentes que condicionan la elevación de las presiones mediante la ecuación del movimiento del sistema respiratorio permite realizar modificaciones a los parámetros programados del ventilador para mantener una ventilación protectora.

Conclusión: la toma de decisiones basada en la ecuación del movimiento del sistema respiratorio permite realizar ajustes en los parámetros ventilatorios según las características y enfermedades del paciente bajo ventilación mecánica.
Bondadoso y pecador, el significado de mi experiencia de liderazgo en la educación médica. Una indagación narrativa autoetnográfica
Authors: Vergel John
Published: August 2022

Introducción: se ha puesto mucho énfasis en investigar los atributos del líder educativo, pero se ha prestado menos atención en comprender su experiencia. No obstante, explorar dicha experiencia es crucial para reducir la brecha teórico-práctica entre lo que esa persona debería ser/hacer y lo que en la práctica realmente es/hace.
Objetivo: comprender el significado de mi experiencia como líder educativo de un programa de medicina desde la perspectiva pragmática y personal.
Métodos: adopto un enfoque cualitativo, soportado en la indagación narrativa autoetnográfica. Consecuentemente, este texto fue escrito en primera persona. Recolecté un diario de reflexión y realicé cuatro (auto)entrevistas semiestructuradas para recordar eventos críticos de mi experiencia. Analicé las transcripciones identificando personajes, temporalidad, locaciones, aspectos culturales, tensiones y metáforas de mi historia de vida.
Resultados: compuse cuatro tramas resonantes representadas con las siguientes metáforas: a) el pecado original, b) el mal hijo se marcha de casa, c) a las serpientes hay que cortarles la cabeza, y d) y llegó el apocalipsis.
Conclusión: esta narrativa me condujo a un entendimiento distinto del concepto de liderazgo en la educación médica. Esta nueva comprensión tiene en cuenta las tensiones intrapersonales y la forma en que el uso del poder te cambia a ti mismo en la práctica, en lugar de cambiar a los demás, como teóricamente se asume.
Relación entre deficiencia de vitamina D con el estado nutricional y otros factores en adultos de la región interandina del Ecuador
Authors: Robles Rodríguez Julieta Beatriz , Pazmiño Karina , Jaramillo Alexandra , Castro José , Chávez Melanie , Granadillo Emily , Rodríguez Alejandro
Published: August 2022

Antecedentes: la deficiencia de vitamina D es un problema de salud pública asociado con diversas enfermedades en población adulta que podría estar relacionado con la actividad laboral. Objetivo: relacionar la deficiencia de vitamina D con el estado nutricional, la composición corporal y factores sociodemográficos en trabajadores de Quito, Ecuador. Materiales y métodos: estudio transversal en 245 trabajadores. Se evaluó vitamina D sérica por inmunofluorescencia, estado nutricional por Índice de Masa Corporal, composición corporal por bioimpedancia y otros factores sociodemográficos. Se usó regresión logística para identificar asociaciones entre deficiencia de vitamina D y variables de interés. Resultados: el 76 % de la población presentó deficiencia de vitamina D. El riesgo cardiovascular por circunferencia abdominal, edad y sexo estaban asociados con deficiencia de vitamina D. Por cada centímetro de incremento en la circunferencia abdominal, la prevalencia de deficiencia de vitamina D incrementaba 4,4 % (OR = 1,044 IC95 %:1,001-1,008; p = 0,043). Por cada año de edad de incremento, la prevalencia disminuía 4,1 % (OR = 0,96; IC95 %:0,93-0,99; p = 0,030). Las mujeres mostraron 2,33 veces más deficiencia de vitamina D en comparación con los hombres (OR = 2,33 IC95 %:1,28-6,94 p = 0,011). Conclusión: la deficiencia de vitamina D es alta en los trabajadores de la ciudad de Quito. Aparentemente, mujeres jóvenes con una circunferencia abdominal alta tienen mayor probabilidad de presentar esta deficiencia. El exceso de peso podría estar reduciendo la actividad física al aire libre, lo que disminuye a su vez la exposición a la luz solar y esto deriva en deficiencia de vitamina D.
Pensamiento y existencia de don Quijote, una lectura desde el cogito cartesiano
Authors: Pinzón Juliana
Published: August 2022

En el presente artículo de reflexión se compara el concepto del cogito ergo sum cartesiano con el personaje don Quijote de La Mancha a partir de los conceptos de pensamiento y existencia, buscando ofrecer una respuesta a la pregunta: ¿hay en el Quijote una representación del cogito cartesiano? Inicialmente, se plantea una breve conceptualización de cómo se comprende el cogito al interior de las Meditaciones acerca de la filosofía primera (2009), proponiendo dos momentos a partirde la idea de Dios. Posteriormente, se hace un análisis del pensamiento y la existencia que sustentan la vida de don Quijote en la novela cervantina, a través de la mirada del narrador, los demás personajes, la figura del encantador, los discursos y elucubraciones mismas del protagonista. Finalmente, en las conclusiones, se comparan: la comprensión y el funcionamiento de la idea de Dios o del encantador; la distinción entre un pensamiento abstracto y uno concreto; la relación entre pensar y existir. Por último, se responde a la pregunta inicial.
Da afirmação enfática à concessividade
Authors: Longhin Sanderléia Roberta
Published: August 2022

Este trabalho investiga os processos diacrônicos de constituição de duas perífrases conjuncionais concessivas do português, bem que e se bem que. Com respaldo teórico da Gramaticalização, sobretudo Traugott e Dasher (2002), Heine e Kuteva (2007), Bybee (2010), e de alguns pressupostos de teorias pragmáticas sobre concessividade, conforme Anscombre (1980), Ducrot (1984), Moeschler e Spengler (1982), Rossari (2014), a questão maior está na compreensão do elo entre a semântica da fonte adverbial, os processos inferenciais instigados contextualmente e o tipo de significado concessivo. As análises são baseadas em uma amostra diacrônica que reúne textos do português antigo ao moderno e em uma metodologia norteada pelos padrões polissêmicos das construções com suas respectivas propriedades distribucionais. Os resultados fornecem evidências de que, embora se trate de dois fenômenos de mudança que emergem em diferentes estados de língua e em contextos distintos, ambos têm na fonte o advérbio bem, na função de afirmação enfática, e ambos se especializam na expressão de relações concessivas restritivas. Os dados duplamente compatíveis com as interpretações fonte e alvo indiciam processos graduais da pragmática à semântica, nos quais as noções de polifonia e hierarquia argumentativa são importantes.
figuras na argumentação
Authors: Mazzola Renan , Kogawa João
Published: August 2022

Neste trabalho, analisamos as figuras de retórica que equipam a argumentação de seis candidatos à presidência da república no contexto dos debates pré-eleitorais da corrida presidencial de 2018. Partimos da hipótese de que as figuras (ornatos, em latim) não são somente enfeites da linguagem, mas verdadeiros equipamentos de persuasão. Elaboramos, para este fim, três perguntas de pesquisa: a) como as figuras equipam a linguagem? b) de que forma as figuras podem construir a persuasão no discurso ndeliberativo político? c) como as figuras integram tipos de argumentos para equipá-los? Fundamentamos nossa pesquisa nos domínios da retórica, da argumentação e do discurso, particularmente nas obras de Aristóteles (2013), Barthes (2001), Perelman e Tyteca (2014), Fiorin (2020), Plantin (2008), Amossy (2020), Abreu (2009), Ferreira (2015) e Courtine (1990). Metodologicamente, a pesquisa possui caráter bibliográfico-documental, de natureza descritiva e explicativa. Nosso corpus é composto por recortes dos discursos dos seis candidatos à república no debate pré-eleitoral televisionado da RedeTV, realizado em 17 de agosto de 2018 e disponível no canal da emissora na plataforma de compartilhamento de vídeos Youtube. No que se refere aos resultados, demonstramos que a presença de determinadas figuras de retórica – como a metáfora, a metonímia, a personificação, a comparação, etc. – contribuíram para a intensificação da argumentação desses candidatos, aumentando a eficácia dos argumentos e, consequentemente, contribuindo para a adesão dos espíritos.
Karaciğer biyopsisi endikasyonlarında ve histopatolojik tanılarda yıllar içindeki değişim: 7 yıllık tek merkez deneyimi
Authors: DANIŞ Nilay , GÜNŞAR Fulya , YILMAZ Funda , NART Deniz , TURAN İlker , KARASU Zeki , ERSÖZ Galip , ÖZÜTEMİZ Ahmet Ömer , AKARCA Ulus Salih
Published: August 2022

Giriş ve Amaç: Bu çalışmanın amacı 7 yıl boyunca aynı merkezde karaciğer biyopsisi yapılan hastaların tanılarını ortaya koymak ve Fibroscan® kullanıma girdikten sonra karaciğer biyopsisi yapılma endikasyonlarında bir değişiklik olup olmadığını araştırmaktır. Gereç ve Yöntem: Haziran 2014 - Aralık 2020 tarihleri arasında tek bir merkezde perkütan olarak yapılmış olan karaciğer biyopsileri retrospektif olarak taranmıştır. Bulgular: Toplam 1783 hastaya karaciğer biyopsisi yapılmıştır. Bu hastaların yaş ortalaması 48.7 ± 14.4 olup ortanca yaş 50’dir. Hastaların 996’sı kadın (%55.9); 787’si erkektir (%44.1). En fazla karaciğer biyopsisi yapılan hastalık hepatit B virüs enfeksiyonu (n: 400) olurken bunu non-alkolik yağlı karaciğer hastalığı (n: 298) izlemektedir. Karaciğer biyopsisi yapılan tüm biyopsi hastalarının; 2014 yılında %31.8’ini hepatit B virüsü; %11.1’ini non-alkolik yağlı karaciğer hastalığı; %5’ini hepatit C virüsü; %18.4’ünü etiyolojisi net olarak ortaya konulamayan hastalıklar; %3.1’ini otoimmün hepatit oluştururken, 2020 yılında bu oranlar sırasıyla hepatit B virüsü için %17.1; non-alkolik yağlı karaciğer hastalığı için %23.7; hepatit C virüsü için %0; etiyolojisi bilinmeyen bilinmeyen hastalıklar için %6.6; otoimmün hepatit için %11.8 bulunmuştur. Sonuç: Tek bir merkezin 7 yıllık verisine göre, karaciğer biyopsisi yapılma endikasyonları ve histopatolojik tanılar içinde hepatit B virüsü ve hepatit C virüsü sayısal ve oransal giderek azalmakta, non-alkolik yağlı karaciğer hastalığı sayısal ve oransal olarak artmakta, bilinmeyen etiyolojiler sayısal ve oransal olarak azalmakta, otoimmün hepatit ise sayısal olarak artmayıp oransal olarak artmaktadır.
Vigilancia de la resistencia a los antimicrobianos en cepas de Neisseria gonorrhoeae aisladas en Paraguay. Año 2021
Authors: Falcón Myrian , Melgarejo-Touchet Nancy , Brítez Mariel , Busignani Sofia , Mendoza Fabiola , Velázquez Gladys , Jara Adriana , Álvarez Mercedes , Páez Diana , González Rogelio , Méndez Erika , Velázquez Dolly , Medina Betania , Terol Marta , Rojas Carolina , Takahasi Vivian , Arza Fátima , Cuevas Deili , Pereira Jazmín , Irala Juan , Alvarenga Perla , López Evelyn
Published: August 2022

Neisseria gonorrhoeae (N. gonorrhoeae) es el agente causal de la gonorrea, infección de transmisión sexual (ITS) que corresponde a la segunda causa más frecuente de ITS a nivel mundial, provocando una alta morbilidad y costo en atención de salud. En las últimas décadas han aumentado los reportes a nivel mundial de cepas resistentes a penicilina, sulfonamidas, tetraciclina, macrólidos y fluoroquinolonas, y más recientemente a azitromicina y cefalosporinas de espectro extendido como ceftriaxona y cefixima. El objetivo principal de este estudio fue determinar la sensibilidad a los antimicrobianos en cepas de N. gonorrhoeae que fueron enviadas al Laboratorio Central de Salud Pública (LCSP), por los centros colaboradores de la Red de Vigilancia Laboratorial de la Resistencia a los Antimicrobianos (RAM). Para ello, se realizó un estudio prospectivo de corte transversal de enero a diciembre de 2021. Se caracterizaron 128 cepas como N. gonorrhoeae a las cuales se le realizaron pruebas de susceptibilidad obteniéndose 48% de resistencia y 52% de sensibilidad intermedia a penicilina. El 70% presentó resistencia a ciprofloxacina y el 19% a tetraciclina. Se obtuvo 100% de sensibilidad a ceftriaxona y cefixima. El fenotipo de resistencia de mayor prevalencia fue QRNG, asociado con resistencia a ciprofloxacina, seguido del fenotipo PPNG-QRNG, asociado con resistencia a penicilina y ciprofloxacina. Ante estos hallazgos y frente a la emergencia mundial de la resistencia a los antimicrobianos, especialmente de cefalosporinas de espectro extendido, se recomienda que los laboratorios de bacteriología fortalezcan la vigilancia para apoyar la detección de casos y proporcionar el tratamiento adecuado.
Características, nivel de conocimiento y acciones de circulantes sobre prevención de infecciones del sitio quirúrgico
Authors: Romero-Vallejos Anyelo David , Recalde-Marrés Pablo Leonardo
Published: August 2022

La infección de la herida quirúrgica y las complicaciones que de ella se derivan han constituido un hecho inseparable a la práctica quirúrgica desde sus rudimentarios comienzos hasta la actualidad. La aparición de una infección de la herida quirúrgica es el resultado de la interacción entre gérmenes patógenos existentes y el huésped. El estudio tuvo por objetivo determinar el conocimiento e intervenciones de circulantes sobre prevención de infecciones del sitio quirúrgico. Experiencias en hospital privado de Paraguay, 2022. La investigación fue observacional, descriptiva, corte transversal, con enfoque cuantitativo. La población constó de 18 circulantes seleccionados mediante muestreo no probabilístico a criterio. Se utilizó una encuesta, empleando como técnica la entrevista, y el instrumento fue el cuestionario. Los datos fueron tabulados en planillas electrónicas Microsoft Excel 2010, interpretados por EpiInfo 7.2.0.1. A partir de los resultados se construyeron tablas y gráficos elaborados en Microsoft Excel. La mayoría tenía entre 21 a 30 años de edad, predominaba el sexo femenino, universidad de egreso, predominantemente pública y tenían una antigüedad de entre 1 a 5 años. Se determinó que la gran mayoría de los sujetos de estudio poseía un conocimiento amplio sobre apreciaciones generales en cuanto a prevención de infecciones del sitio quirúrgico, y que la totalidad de los mismos realizaban las acciones para prevenir infecciones del sitio quirúrgico.