bluuebunny/crossref_metadata_2025 huggingface dataset:
Determinants of fertility in Malawi: Does women autonomy dimension matter?
Authors: Forty James , Navaneetham Kannan , Letamo Gobopamang
Published: August 2022

Background

Power inequality within the household and sexual relationships is linked to poor reproductive health. Malawi Government through National Sexual and Reproductive Health and Rights policy is committed to women empowerment as well fertility reduction. However, there is limited evidence in Malawi regarding whether women’s autonomy in the household is an independent determinant of fertility. With this background, the aim of this study is to investigate whether women’s autonomy in the household is a determinant of fertility in a poor socioeconomic and cultural setting.

Methods

This study used Malawi Demographic and Health Survey, 2015–2016. A multivariable Poisson regression model was used to investigate if women’s autonomy in the household in Malawi determines fertility. The outcome measure, children ever born, was used as a measure of fertility. Women’s autonomy was measured with two dimensions, such as women’s household related decision makings and women’s sexual autonomy. The individual recode and household recode were merged for the analysis. The final study sample was 15,952 women who were cohabiting or married at the time of the survey.

Results

The level of autonomy among women in the household related decisions and sexual autonomy was 49.1% and 64.0% respectively. Controlling for covariates, the study found no significant association between women’s autonomy dimensions in the household and number of children ever born. On the other hand, living in urban area (IRR = 0.91, CI 0.88–0.93); having less than tertiary education thus, no education (IRR = 1.83, CI 1.67–1.99) or primary education (IRR = 1.55, CI 1.42–1.69) or secondary education (IRR = 1.23, CI 1.13–1.33); poor households (IRR = 1.05, CI 1.01–1.09), starting cohabiting at the age of 19 years or less (AIRR = 1.15, CI 1.13–1.18) and not using modern contraceptive methods (AIRR = 1.17, CI 1.15–1.19) were significantly associated with fertility.

Conclusions and recommendations

Though women’s autonomy does not have independent effect on fertility, it may be interacting with other sociocultural norms prevailing in the society. The study recommends that the Government of Malawi should come up with economic hardship emancipation policy for poor households. The government should also come up with a girl-child secondary school completion policy. Furthermore, the government should accelerate the implementation, monitoring and evaluation of National Gender Policy to ensure the women empowerment/autonomy is having positive effect at all level including the household.
The efficacy of metal nanocomposite (Fe3O4/CuO/ZnO) to ameliorate the toxic effects of ochratoxin in broilers
Authors: Al Shap Nagla F. , El-Sherbeny Eman M. El. , El Masry Dalia M. A.
Published: August 2022

Background

The study aimed to investigate the effectiveness of different doses of metal nanocomposite (MNc) (Fe3O4/CuO/ZnO) lower than its cytotoxic level in order to overcome or minimize the ochratoxin (OTA) adverse effects in broilers fed on contaminated ration.

The study conducted on 120 one-day old chicks which were divided into equal 6 groups; G1: negative control, G2: positive control (fed on OTA 17 ppb), G3& G4 (fed MNc only with low and high doses respectively). The rest two groups G5 & G6 (treatment groups) were fed on OTA, post induced ochratoxification, treated with low and high doses respectively.

Results

Body weight gain and heamatocellular elements in both treated groups increased significantly than control. Serum phagocytic nitric oxide levels were increased significantly in both treated groups than control groups.

Prothrombin time (PT), Alanine aminotransferase (ALT) and gamma-glutamyltransferase (GGT) activities decreased significantly (P < 0.05) in both treated groups than intoxicated control group (G2) but still higher than non-intoxicated control group (G1). Total protein, albumin, globulin, calcium and phosphorus increased significantly in both treated groups than intoxicated control group. Kidney function tests showed significant improvement in both treated groups than intoxicated control group.

Antioxidant study revealed that malondialdehyde (MDA) decreased significantly in treated groups than intoxicated control group. Ochratoxin residue decreased significantly in treated groups. Metal residues in tested liver and muscle of treated groups showed no-significant difference with non-intoxicated control group (G1) at the experiment’s end.

In conclusion, feeding either low or high doses of MNc to broilers were significantly counteracting the negative impacts of OTA or its residue and increase their body weight.
High expression of serine and arginine-rich splicing factor 9 (SRSF9) is associated with hepatocellular carcinoma progression and a poor prognosis
Authors: Zhang Guoshun , Liu Bin , Shang Hua , Wu Guikai , Wu Diyang , Wang Liuqing , Li Shengnan , Wang Zhiyuan , Wang Suying , Yuan Juxiang
Published: August 2022

Background

Serine and arginine-rich splicing factor 9 (SRSF9) has been linked to the occurrence and progression of various cancers; however, its effects and mechanism of action hepatocellular carcinoma (HCC) have not been reported. In this study, we used a bioinformatics approach and in vitro assays to evaluate the expression of SRSF9 in HCC, its prognostic value, and its underlying regulatory mechanisms, including analyses of related pathways and the role of methylation.

Methods

Transcriptomic and DNA methylation data for 357 HCC cases and 50 paratumor tissues in The Cancer Genome Atlas database were obtained. Additionally, protein expression data for cell lines and tissue samples were obtained from the Human Protein Atlas. The CMap databased was used to predict candidate drugs targeting SRSF9. Various cell lines were used for in vitro validation.

Results

SRSF9 expression was significantly elevated in HCC and was negatively regulated by its methylation site cg06116271. The low expression of SRSF9 and hypermethylation of cg06116271 were both associated with a longer overall survival time. A correlation analysis revealed ten genes that were co-expressed with SRSF9; levels of CDK4, RAN, DENR, RNF34, and ANAPC5 were positively correlated and levels of RBP4, APOC1, MASP2, HP, and HPX were negatively correlated with SRSF9 expression. The knockdown of SRSF9 in vitro inhibited the proliferation and migration of HCC cells and significantly reduced the expression of proteins in the Wnt signaling pathway (DVL2 and β-catenin) and cell cycle pathway (Cyclin D and Cyclin E). A CMap analysis identified two drugs, camptothecin and apigenin, able to target and inhibit the expression of SRSF9.

Conclusions

This study expands our understanding of the molecular biological functions of SRSF9 and cg06116271 and provides candidate diagnostic and therapeutic targets for HCC.
Rational drug combination design in patient-derived avatars reveals effective inhibition of hepatocellular carcinoma with proteasome and CDK inhibitors
Authors: Lim Jhin Jieh , Hooi Lissa , Dan Yock Young , Bonney Glenn K. , Zhou Lei , Chow Pierce K.-H. , Chee Cheng Ean , Toh Tan Boon , Chow Edward K.-H.
Published: August 2022

Background

Hepatocellular carcinoma (HCC) remains difficult to treat due to limited effective treatment options. While the proteasome inhibitor bortezomib has shown promising preclinical activity in HCC, clinical trials of bortezomib showed no advantage over the standard-of-care treatment sorafenib, highlighting the need for more clinically relevant therapeutic strategies. Here, we propose that rational drug combination design and validation in patient-derived HCC avatar models such as patient-derived xenografts (PDXs) and organoids can improve proteasome inhibitor-based therapeutic efficacy and clinical potential.

Methods

HCC PDXs and the corresponding PDX-derived organoids (PDXOs) were generated from primary patient samples for drug screening and efficacy studies. To identify effective proteasome inhibitor-based drug combinations, we applied a hybrid experimental-computational approach, Quadratic Phenotypic Optimization Platform (QPOP) on a pool of nine drugs comprising proteasome inhibitors, kinase inhibitors and chemotherapy agents. QPOP utilizes small experimental drug response datasets to accurately identify globally optimal drug combinations.

Results

Preliminary drug screening highlighted the increased susceptibility of HCC PDXOs towards proteasome inhibitors. Through QPOP, the combination of second-generation proteasome inhibitor ixazomib (Ixa) and CDK inhibitor dinaciclib (Dina) was identified to be effective against HCC. In vitro and in vivo studies demonstrated the synergistic pro-apoptotic and anti-proliferative activity of Ixa + Dina against HCC PDXs and PDXOs. Furthermore, Ixa + Dina outperformed sorafenib in mitigating tumor formation in mice. Mechanistically, increased activation of JNK signaling mediates the combined anti-tumor effects of Ixa + Dina in HCC tumor cells.

Conclusions

Rational drug combination design in patient-derived avatars highlights the therapeutic potential of proteasome and CDK inhibitors and represents a feasible approach towards developing more clinically relevant treatment strategies for HCC.
Protocol for an automated, pragmatic, embedded, adaptive randomised controlled trial: behavioural economics-informed mobile phone-based reminder messages to improve clinic attendance in a Botswanan school-based vision screening programme
Authors: Allen Luke N , Ratshaa Bakgaki , Macleod David , Bolster Nigel , Burton Matthew , Kim Min , Bastawrous Andrew , Ho-Foster Ari , Chroston Hannah , Nkomazana Oathokwa
Published: August 2022

Background

Clinic non-attendance rates are high across the African continent. Emerging evidence suggests that phone-based reminder messages could make a small but important contribution to reducing non-attendance. We will use behavioural economics principles to develop an SMS and voice reminder message to improve attendance rates in a school-based eye screening programme in Botswana.

Methods

We will test a new theory-informed SMS and voice reminder message in a national school-based eye screening programme in Botswana. The control will be the standard SMS message used to remind parents/guardians to bring their child for ophthalmic assessment. All messages will be sent twice. The primary outcome is attendance for ophthalmic assessment. We will use an automated adaptive approach, starting with a 1:1 allocation ratio.

Discussion

As far as we are aware, only one other study has used behavioural economics to inform the development of reminder messages to be deployed in an African healthcare setting. Our study will use an adaptive trial design, embedded in a national screening programme. Our approach can be used to trial other forms of reminder message in the future.

Trial registration

ISRCTN https://doi.org/10.1186/ISRCTN96528723. Registered on 5 January 2022.
Prevention and early treatment of the long-term physical effects of COVID-19 in adults: design of a randomised controlled trial of resistance exercise—CISCO-21
Authors: Morrow A. , Gray Stuart R. , Bayes H. K. , Sykes R. , McGarry E. , Anderson D. , Boiskin D. , Burke C. , Cleland J. G. F. , Goodyear C. , Ibbotson T. , Lang C. C. , McConnachie  , Mair F. , Mangion K. , Patel M. , Sattar N. , Taggart D. , Taylor R. , Dawkes S. , Berry C.
Published: August 2022

Background

Coronavirus disease-19 (COVID-19) infection causes persistent health problems such as breathlessness, chest pain and fatigue, and therapies for the prevention and early treatment of post-COVID-19 syndromes are needed. Accordingly, we are investigating the effect of a resistance exercise intervention on exercise capacity and health status following COVID-19 infection.

Methods

A two-arm randomised, controlled clinical trial including 220 adults with a diagnosis of COVID-19 in the preceding 6 months. Participants will be classified according to clinical presentation: Group A, not hospitalised due to COVID but persisting symptoms for at least 4 weeks leading to medical review; Group B, discharged after an admission for COVID and with persistent symptoms for at least 4 weeks; or Group C, convalescing in hospital after an admission for COVID.

Participants will be randomised to usual care or usual care plus a personalised and pragmatic resistance exercise intervention for 12 weeks. The primary outcome is the incremental shuttle walks test (ISWT) 3 months after randomisation with secondary outcomes including spirometry, grip strength, short performance physical battery (SPPB), frailty status, contacts with healthcare professionals, hospitalisation and questionnaires assessing health-related quality of life, physical activity, fatigue and dyspnoea.

Discussion

Ethical approval has been granted by the National Health Service (NHS) West of Scotland Research Ethics Committee (REC) (reference: GN20CA537) and recruitment is ongoing. Trial findings will be disseminated through patient and public forums, scientific conferences and journals.

Trial registration

ClinicialTrials.gov NCT04900961. Prospectively registered on 25 May 2021
Construction of a domestic wastewater disinfection filter from biosynthesized and commercial nanosilver: a comparative study
Authors: Taher Heba S. , Sayed Rania , Loutfi Asmaa , Abdulla Hesham
Published: August 2022

Purpose

Biosynthesis of nanoparticles is an eco-friendly process and considered one of the most significant aspects of nanotechnology. Silver nanoparticles (Ag NPs) have a better bactericidal activity due to its high surface area to volume ratio. In this paper, Streptomyces sp. U13 (KP109813) was used to biosynthesize silver nanoparticles (Ag NPs) to construct wastewater disinfection filter.

Methods

The biosynthesized nanosilver and a commercially available ink nanosilver were characterized, and their wastewater disinfection efficiency was compared. The nanometrological characteristics of both nanosilver such as structure, shape, and size were investigated using the X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FTIR), high-resolution transmission electron microscope (HR-TEM), and UV-visible spectroscopy.

Result

The results revealed that the biosynthesized and ink Ag NPs were well dispersed and had a spherical shape, with sizes ranged from 5 to 37 nm and from 2 to 26 nm, respectively. To examine the disinfection capabilities, Ag NPs were loaded on two substrates, foam and limestone gravel, and packed into a glass column receiving domestic wastewater. Results showed that Ag NPs attached to limestone gravel eliminate 100% of the coliform bacteria better than foam. Comparing to control columns (without silver), only 50 and 10% reduction of the total coliform in gravel and foam column were achieved, respectively.

Conclusion

This work concluded that the type of substrate controls the amount of Ag NPs loaded on it and thus controls the disinfection process. No significant difference between biosynthesized and ink nanosilver in wastewater disinfection was observed. Using limestone gravel filter loaded with 200 mg/l Ag NPs with contact time of 150 min achieves a complete eradication of coliform bacteria.
A pattern of livestock depredation by snow leopard to the yak herding pastoralist in western Bhutan
Authors: Dorji Phub , Powrel Reta Bahadur
Published: August 2022

The pastoralists co-exist with wild predators and livestock depredation by predators causes an immense impact on the livelihood of the herders and instigates a negative attitude towards the conservation of these wild predators. Yak herders in western Bhutan move from place to place for herding on pasture and they face challenges with livestock predation by top predators like snow leopard (Panthera uncia) and dhole (Cuon aplinus). To investigate patterns of livestock depredation by the snow leopard and determine the attitude of herders towards snow leopard conservation, we conducted a household interview with all 56 itinerant yak herders in the west of Bhutan. Each herd was keeping a mean of 84 (± 29) yaks per herd. Yaks were mainly kept for milk and bulls for breeding and bullocks for meat to sustain their family livelihood. Predation of livestock by predators (42.9%) was among the top problems faced by the yak herders. A total of 398 yaks were lost to snow leopards (78.86%) followed by dhole (18.3%), Himalayan black bear (Ursus thibetanus) (2.7%), and common leopard (Panthera pardus) (0.2%) in the past 5 years (2015–2019). The majority (87.22.8%) of the kills by snow leopards were young yak and most (60.5%) kills were recorded during summer. Snow leopards are considered harmful (73.2%), and herders (71.1%) are not in favour of snow leopard conservation. Herders’ conflict with snow leopards is severe in the current study site, and we recommend social development for conservation programmes like livelihood alternatives for the herders, compensation and insurance schemes, and conservation awareness programmes for the yak herders as an intervention to create harmonic co-existence between the yak herder and the snow leopard.
Mechanical Properties and Microstructure of Basalt Fiber Reinforced Concrete Under the Single-Side Salt-Freezing–Drying–Wetting Cycles
Authors: Zeng Hao , Zhang Jin , Li Yang , Su Xin , Gu CongZhi , Zhang Kai
Published: August 2022

In the past, the salt freezing test does not often accord with the actual service environment of engineering, thus, we designed a test method of single-side salt-freezing–drying–wetting cycles. The mechanical properties and microstructure of ordinary concrete and basalt fiber reinforced concrete were studied. The mechanical property test is aimed at the splitting tensile strength and compressive strength of concrete after different cycles. The microstructure test is to study the hydration products by scanning electron microscope (SEM) and the pore structure of concrete by mercury intrusion porosimetry (MIP) test. The results indicate that the addition of basalt fiber can improve the compactness and pore structure of concrete. It is beneficial to enhance the durability of concrete under single-side salt-freezing–drying–wetting cycles. The improving effect of basalt fiber is better on the splitting tensile strength of concrete than the compressive strength. Basalt fiber exerts the best effect on reducing harmful holes in concrete. However, there is an optimal range of basalt fiber content, the performance of concrete will deteriorate with excessive fiber content. The cycles will destroy the hydration products of concrete and the synergistic effect between hydration products and fibers, but has little effect on the three-dimensional network constructed by basalt fibers. The pore structure of concrete is correlated with the mechanical properties of it under cyclic conditions, which is worth further study.
Microbial functional genes are driven by gradients in sediment stoichiometry, oxygen, and salinity across the Baltic benthic ecosystem
Authors: Broman Elias , Izabel-Shen Dandan , Rodríguez-Gijón Alejandro , Bonaglia Stefano , Garcia Sarahi L. , Nascimento Francisco J. A.
Published: August 2022

Background

Microorganisms in the seafloor use a wide range of metabolic processes, which are coupled to the presence of functional genes within their genomes. Aquatic environments are heterogenous and often characterized by natural physiochemical gradients that structure these microbial communities potentially changing the diversity of functional genes and its associated metabolic processes. In this study, we investigated spatial variability and how environmental variables structure the diversity and composition of benthic functional genes and metabolic pathways across various fundamental environmental gradients. We analyzed metagenomic data from sediment samples, measured related abiotic data (e.g., salinity, oxygen and carbon content), covering 59 stations spanning 1,145 km across the Baltic Sea.

Results

The composition of genes and microbial communities were mainly structured by salinity plus oxygen, and the carbon to nitrogen (C:N) ratio for specific metabolic pathways related to nutrient transport and carbon metabolism. Multivariate analyses indicated that the compositional change in functional genes was more prominent across environmental gradients compared to changes in microbial taxonomy even at genus level, and indicate functional diversity adaptation to local environments. Oxygen deficient areas (i.e., dead zones) were more different in gene composition when compared to oxic sediments.

Conclusions

This study highlights how benthic functional genes are structured over spatial distances and by environmental gradients and resource availability, and suggests that changes in, e.g., oxygenation, salinity, and carbon plus nitrogen content will influence functional metabolic pathways in benthic habitats.
Perception of facing life's challenges in patients with spinal cord injury in Iran: a qualitative study
Authors: Mohammadi Fateme , Oshvandi Khodayar , Bijani Mostafa , Borzou Seyed Reza , khodaveisi Masoud , Masoumi Seyedeh Zahra
Published: August 2022

Background

Spinal cord injury (SCI) is one of the most serious types of physical trauma and has become a major life-threatening condition in the recent decade. It is essential that the life perception and experiences of patients with SCI be studied and evaluated in different cultural contexts so that their needs and the challenges they face can be properly determined. The present study aims to explore the how patients with SCI in the south of Iran perceive facing life's challenges.

Methods

The present study is a qualitative research with a descriptive phenomenological design. Participants were identified through purposive sampling of patients with spinal cord injury admitted to two state hospitals affiliated with a university of medical sciences in western Iran. The researchers collected data using semi-structured, in-depth interviews with 25 SCI patients conducted between August and October 2021. Data was analyzed according to Colaizzi's method using MAXQDA v. 2007.

Results

Analysis of the data led to the emergence of three themes and nine sub-themes. The three main themes were emotional shock (crisis making and mental rumination, persistent depressive disorder, pitying behaviors, fear of the future), loss of dignity (poor self- care, sexual dysfunction, loss of job and educational status), and lack of effective support (lack of financial institutions and sponsors, lack of social support).

Conclusion

Patients with spinal cord injuries face various issues in their care and social lives. Attention to their psycho-emotional needs along with comprehensive health support play key roles in generating a sense of self efficacy and promoting the mental well-being and dignity of patients with spinal cord injuries. Accordingly, healthcare administrators and caregivers are recommended to provide more comprehensive health support to SCI patients to meet their needs more effectively.
Reduced Wheel Running via a High-Fat Diet Is Reversed by a Chow Diet with No Added Benefit from Fecal Microbial Transplants
Authors: LETSINGER AYLAND C. , YANG FANG , MENON RANI , LITTLE-LETSINGER SARAH E. , GRANADOS JORGE Z. , BREIDENBACH BRIANNE , IYER ANJUSHREE R. , PADOVANI TATIANA CASTRO , NAGEL EDWARD C. , JAYARAMAN ARUL , LIGHTFOOT J. TIMOTHY
Published: August 2022

Purpose

Chronic overfeeding via a high-fat/high-sugar (HFHS) diet decreases wheel running and substantially alters the gut metabolome of C57BL/6J mice. In this study, we tested the hypothesis that fecal microbial transplants can modulate the effect of diet on wheel running.

Methods

Singly housed, 6-wk-old male C57BL/6J mice were fed either a grain-based diet (CHOW) or HFHS diet and provided a running wheel for 13 wk. Low-active, HFHS-exposed mice were then either switched to a CHOW diet and given an oral fecal microbial transplant from mice fed the CHOW diet, switched to a CHOW diet and given a sham transplant, or remained on the HFHS diet and given a fecal microbial transplant from mice fed the CHOW diet. Total wheel running, nutrient intake, body composition, fecal microbial composition, fecal metabolite composition, and liver steatosis were measured at various times throughout the study.

Results

We found that an HFHS diet decreases wheel running activity, increases body fat, and decreases microbial alpha diversity compared with a CHOW diet. Improvements in wheel running, body composition, and microbial alpha diversity were accomplished within 2 wk for mice switched from an HFHS diet to a CHOW diet with no clear evidence of an added benefit from fecal transplants. A fecal transplant from mice fed a CHOW diet without altering diet did not improve wheel running or body composition. Wheel running, body composition, fecal microbial composition, fecal metabolite composition, and liver steatosis percentage were primarily determined by diet.

Conclusions

Our results suggest that diet is a primary mediator of wheel running with no clear effect from fecal microbial transplants.
Providing bilingual COVID-19 educational material for the border region during the pandemic
Authors: Nagineni Lokesh , Kichena Shravya , Bateman Emily , Takigawa Kei , Holland Nathan , Perry Cynthia , Hogg Tanis , Cervantes Jorge , Delarosa Jose Manuel , Longhurst Melanie , Janssen Herb , Pfarr Curt , Manglik Niti , Ayoubieh Houriya , Chacon Jessica
Published: August 2022

Background: Studies show that the introduction of early public health interventions correlates with decreased rates of transmission and reductions in mortality resulting from epidemic or pandemic events.

Methods: Medical educators and students from Texas Tech University Health Sciences Center El Paso created the El Paso-Health Education and Awareness Team (EP-HEAT) to educate at-risk populations in the border region. The English and Spanish material created by EP-HEAT included information about disease symptoms, transmission, preventive measures and mental health resources. An online, anonymous survey was distributed to attendees to obtain feedback on the provided coronavirus disease (COVID-19) educational material.

Results: The results showed that over 90% of participants agreed that the informational pamphlets increased their awareness and knowledge of COVID-19.

Conclusions: In this study, educators and students created COVID-19-related material in virtual workshops and pamphlets, designed to increase knowledge about COVID-19, mitigate the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and how to address mental health issues related to the pandemic. A majority of participants agreed or strongly agreed that the COVID-19 material developed by EP-HEAT helped raise awareness, understanding, and the importance of education about SARS-CoV-2/COVID-19.
Optical format interconversion nodes between OOK and QPSK enabled by a reconfigurable two-dimensional vector mover
Authors: Cui Jiabin , Ji Yuefeng , Lu Guo-Wei , Wen Huashun , Zhai Kunpeng , Wang Xin , Zhu Ninghua
Published: August 2022

An optical format interconversion scheme between on-off keying (OOK) and quadrature phase shift keying (QPSK) is proposed and verified in this paper. The conversion system mainly consists of a coherent vector combiner and a reconfigurable two-dimensional (2D) vector mover. As a key element of the proposed conversion system, the 2D vector mover is implemented by a non-degenerate phase-sensitive amplifier (PSA). The operating principle and theoretical derivations of the PSA-based 2D vector mover are fully introduced. The reconfigurable transfer characteristics of the vector mover are analyzed under different parameter settings to exhibit the flexible 2D moving function. The signal constellations, eye diagrams, spectrum, error vector magnitudes, and bit error ratios are estimated and depicted to validate the proposed idea. With the input signal-to-noise ratios of 20 dB and 25 dB, error-free conversions are achieved between 50G Baud OOK and QPSK. The scheme proposed in this paper fills the lack of the one-to-one interconversion between OOK and QPSK, and has potential applications in optical interconnect nodes, across-dimensional optical transmissions, and flexible optical transceivers.
Dual-path joint correction network for underwater image enhancement
Authors: Zhang Dehuan , Shen Jiaqi , Zhou Jingchun , Chen Erkang , Zhang Weishi
Published: August 2022

Acquired underwater images often suffer from severe quality degradation, such as color shift and detail loss due to suspended particles’ light absorption and scattering. In this paper, we propose a Dual-path Joint Correction Network (DJC-NET) to cope with the above degenerate issues, preserving different unique properties of underwater images in a dual-branch way. The design of the light absorption correction branch is to improve the selective absorption of light in water and remove color distortion, while the light scattering correction branch aims to improve the blur caused by scattering. Concretely, in the light absorption correction path, we design the triplet color feature extraction module, which balances the triplet color distribution of the degraded image through independent feature learning between R, G, and B channels. In the light scattering correction path, we develop a dual dimensional attention mechanism to extract the texture information from the features, aiming to recover sufficient details by more effective feature extraction. Furthermore, our method utilizes the multi-scale U-net to adaptively fusion features from different paths to generate enhanced images. Extensive visual and objective experimental results demonstrate that our method outperforms state-of-the-art methods in various underwater scenes.
Optical frequency domain polarimetry for distributed polarization crosstalk measurement beyond a 110 dB dynamic range
Authors: Yu Zhangjun , Zhuang Qinqin , Lin Yuhao , Lin Yicheng , Lin Cuofu , Rang Bowei , Yuan Yonggui , Yang Jun , Wen Kunhua , Xu Pengbai , Wang Yuncai , Qin Yuwen
Published: August 2022

In this Letter, we present optical frequency domain polarimetry (OFDP), a novel, to the best of our knowledge, distributed polarization crosstalk (DPC) measurement method with a dynamic range beyond 110 dB. It is based on swept-wavelength interferometry using a tunable laser source (TLS) and a delay line interferometer (DLI). The ultrahigh dynamic range is achieved by optimizing the sweep rate of the TLS and the optical path difference of the DLI. Using OFDP, we measured polarization crosstalk below –105 dB in a LiNbO3 waveguide modulator for the first time. OFDP will play a significant role in developing and fabricating high-performance polarization devices.
Epitaxial Nd3+:YAG/YAG waveguide laser
Authors: Sarnecki Jerzy , Podniesiński Dariusz , Nakielska Magdalena
Published: August 2022

Solid state lasers with a planar Nd,Lu,Ga:YAG/YAG waveguide structure have been created using liquid phase epitaxy. The method used to produce such epitaxial structures ensures obtaining Nd3+ ions concentration in the range from 1 at.% to 3 at.% in waveguiding thin films. Spectroscopic characterization of active films was performed. The Nd,Lu,Ga:YAG waveguide for each neodymium ions concentration exhibited lasing operation at room temperature at a wavelength of 1064 nm with impulse laser diodes pumping at 808 nm and 885 nm. The maximum average output power Pav = 2.6 mW and 72.2% slope efficiency was obtained for 1 at.% Nd3+ waveguide pumped at 885 nm.
Regimes and mechanisms of transient amplification in abstract and biological neural networks
Authors: Christodoulou Georgia , Vogels Tim P. , Agnes Everton J.
Published: August 2022

Neuronal networks encode information through patterns of activity that define the networks’ function. The neurons’ activity relies on specific connectivity structures, yet the link between structure and function is not fully understood. Here, we tackle this structure-function problem with a new conceptual approach. Instead of manipulating the connectivity directly, we focus on upper triangular matrices, which represent the network dynamics in a given orthonormal basis obtained by the Schur decomposition. This abstraction allows us to independently manipulate the eigenspectrum and feedforward structures of a connectivity matrix. Using this method, we describe a diverse repertoire of non-normal transient amplification, and to complement the analysis of the dynamical regimes, we quantify the geometry of output trajectories through the effective rank of both the eigenvector and the dynamics matrices. Counter-intuitively, we find that shrinking the eigenspectrum’s imaginary distribution leads to highly amplifying regimes in linear and long-lasting dynamics in nonlinear networks. We also find a trade-off between amplification and dimensionality of neuronal dynamics, i.e., trajectories in neuronal state-space. Networks that can amplify a large number of orthogonal initial conditions produce neuronal trajectories that lie in the same subspace of the neuronal state-space. Finally, we examine networks of excitatory and inhibitory neurons. We find that the strength of global inhibition is directly linked with the amplitude of amplification, such that weakening inhibitory weights also decreases amplification, and that the eigenspectrum’s imaginary distribution grows with an increase in the ratio between excitatory-to-inhibitory and excitatory-to-excitatory connectivity strengths. Consequently, the strength of global inhibition reveals itself as a strong signature for amplification and a potential control mechanism to switch dynamical regimes. Our results shed a light on how biological networks, i.e., networks constrained by Dale’s law, may be optimised for specific dynamical regimes.
Findings of a community screening programme for human cystic echinococcosis in a non-endemic area
Authors: Mutwiri Titus , Magambo Japhet , Zeyhle Eberhard , Muigai Anne W. T. , Alumasa Lorren , Amanya Fredrick , Fèvre Eric M. , Falzon Laura C.
Published: August 2022

Cystic Echinococcosis (CE) is a zoonosis caused by infection with the larval stages of the taeniid cestodes of the species complex Echinococcus granulosus sensu lato. It is prevalent among transhumant communities in East Africa, including those residing in northern Kenya. The movement of livestock from these regions of high incidence to areas of low incidence creates an indirect risk of disease spill-over to humans. To assess possible establishment of the CE life cycle outside known endemic regions, we used a portable ultrasound scanner to screen for the presence of human CE in Bungoma County of western Kenya, an area which imports substantial numbers of cattle for slaughter from neighbouring pastoralist regions. Eight sentinel sites were purposively selected based on their proximity to slaughterhouses handling animals introduced from pastoralist regions, and necessary permissions to conduct the study were sought. Regression analyses were conducted to identify risk factors associated with the presence of abdominal and cystic lesions (CL). In total, 1002 participants were screened; of these, 654 (65.3%) were female and the median age was 43. Farming (n = 403; 43.4%) was the most frequent occupation, followed by professional (i.e. on regular salary) (n = 215; 23.1%), and business (n = 207; 22.3%) categories. Sixty-seven participants (6.7%) had abnormal ultrasound findings, of these, 7 (1.1%) had simple liver cysts/CL, as per WHO classification. As such, their outcome was inconclusive and they were not put on treatment but advised to attend follow-up investigations in a referral health facility. Other abnormal findings included splenomegaly (n = 14), ovarian cysts (n = 14), uterine fibroids (n = 10), polycystic kidneys (n = 6), and benign prostatic hyperplasia (n = 6). Age was unconditionally associated with the presence of presumptive CL. These results contribute to CE baseline data while providing insights on the implementation of ultrasound diagnosis in the field, as recommended by the WHO for targeted control of echinococcosis by 2030.
Facilitating high quality acute care in resource-constrained environments: Perspectives of patients recovering from sepsis, their caregivers and healthcare workers in Uganda and Malawi
Authors: Limbani Felix , Kabajaasi Olive , Basemera Margaret , Gooding Kate , Kenya-Mugisha Nathan , Mkandawire Mercy , Rusoke Davis , Jacob Shevin T. , Katahoire Anne Ruhweza , Rylance Jamie ,   on behalf of the African Research Collaboration on Sepsis, Patient Experience Study Group
Published: August 2022

Sepsis is a major global health problem, especially in sub-Saharan Africa. Improving patient care requires that healthcare providers understand patients’ priorities and provide quality care within the confines of the context they work. We report the perspectives of patients, caregivers and healthcare workers regarding care quality for patients admitted for sepsis to public hospitals in Uganda and Malawi. This qualitative descriptive study in two hospitals included face-to face semi-structured interviews with purposively selected patients recovering from sepsis, their caregivers and healthcare workers. In both Malawi and Uganda, sepsis care often occurred in resource-constrained environments which undermined healthcare workers’ capacity to deliver safe, consistent and accessible care. Constraints included limited space, strained; water, sanitation and hygiene (WASH) amenities and practices, inadequate human and material resources and inadequate provision for basic needs including nutrition. Heavy workloads for healthcare workers strained relationships, led to poor communication and reduced engagement with patients and caregivers. These consequences were exacerbated by understaffing which affected handover and continuity of care. All groups (healthcare workers, patients and caregivers) reported delays in care due to long queues and lack of compliance with procedures for triage, treatment, stabilization and monitoring due to a lack of expertise, supervision and context-specific sepsis management guidelines. Quality sepsis care relies on effective severity-based triaging, rapid treatment of emergencies and individualised testing to confirm diagnosis and monitoring. Hospitals in resource-constrained systems contend with limitations in key resources, including for space, staff, expertise, equipment and medicines, in turn contributing to gaps in areas such as WASH and effective care delivery, as well as communication and other relational aspects of care. These limitations are the predominant challenges to achieving high quality care.
Prevalence of brucellosis in livestock keepers and domestic ruminants in Baringo County, Kenya
Authors: Lokamar Peter N. , Kutwah Moses A. , Munde Elly O. , Oloo Dickens , Atieli Harrysone , Gumo Sussy , Akoko James M. , Ouma Collins
Published: August 2022

Brucellosis is widely distributed in more than 170 countries around the world, where it poses a huge threat to animal husbandry and human health. Brucellosis is a worldwide re-emerging zoonotic disease that poses serious public health problems in many developing countries including Kenya. However, prevalence of brucellosis has not been determined in Baringo County, Kenya, yet there is a continuous movement of cattle resulting from trade and grazing, thus predisposing many herds to brucellosis infection. We investigated the sero-prevalence of brucellosis in humans and domestic ruminants: sheep, goats, cattle and camels among livestock keeping communities in Baringo County, Kenya. In addition, we analyzed the seropositive samples for molecular detection of Brucella species. The study adopted a cross-sectional survey using quantitative data collection methods. The diagnosis was carried out using a competitive enzyme-linked immunosorbent assay (c-ELISA) and the real-time PCR assays. The sero-prevalence of brucellosis among human blood samples was 0.6% (n = 4/640) in Baringo County. About 22.30% (n = 143/640) of animal blood samples examined tested positive for Brucella genus-specific ELISA test. Cattle had a high prevalence of 22.88% (n = 93/322) followed by camels 20.00% (n = 21/105), goats 15.48% (n = 24/155) and subsequently sheep at 8.62% (n = 5/58). Overall, 7.5% (n = 6/80) of the seropositive samples amplified with the genus-specific primers. Brucella melitensis was detected in one out of the six genus positive samples, while none amplified with the B. abortus target. Even though there was high prevalence of brucellosis among livestock in Baringo County, the highest prevalence was invariably noted in cattle, followed by camels, goats and sheep, respectively. Livestock keepers had low prevalence of brucellosis. This implies that there was low risk of transmission of brucellosis between livestock keepers and their livestock.
RAD51 is a druggable target that sustains replication fork progression upon DNA replication stress
Authors: Feu Sonia , Unzueta Fernando , Ercilla Amaia , Pérez-Venteo Alejandro , Jaumot Montserrat , Agell Neus
Published: August 2022

Solving the problems that replication forks encounter when synthesizing DNA is essential to prevent genomic instability. Besides their role in DNA repair in the G2 phase, several homologous recombination proteins, specifically RAD51, have prominent roles in the S phase. Using different cellular models, RAD51 has been shown not only to be present at ongoing and arrested replication forks but also to be involved in nascent DNA protection and replication fork restart. Through pharmacological inhibition, here we study the specific role of RAD51 in the S phase. RAD51 inhibition in non-transformed cell lines did not have a significant effect on replication fork progression under non-perturbed conditions, but when the same cells were subjected to replication stress, RAD51 became necessary to maintain replication fork progression. Notably, the inhibition or depletion of RAD51 did not compromise fork integrity when subjected to hydroxyurea treatment. RAD51 inhibition also did not decrease the ability to restart, but rather compromised fork progression during reinitiation. In agreement with the presence of basal replication stress in human colorectal cancer cells, RAD51 inhibition reduced replication fork speed in these cells and increased γH2Ax foci under control conditions. These alterations could have resulted from the reduced association of DNA polymerase α to chromatin, as observed when inhibiting RAD51. It may be possible to exploit the differential dependence of non-transformed cells versus colorectal cancer cells on RAD51 activity under basal conditions to design new therapies that specifically target cancer cells.
Parent-mediated play‐based interventions to improve social communication and language skills of preschool autistic children: A systematic review and meta-analysis protocol
Authors: Deniz Emre , Francis Gill , Torgerson Carole , Toseeb Umar
Published: August 2022

Early years interventions have shown to be effective in improving the social communication and language skills of autistic children. Therefore, various play-based interventions have been developed to support those developmental areas of autistic children. Although researchers have previously reported the overall effectiveness of different types of play-based interventions on the social communication and language skills of autistic children, no previous systematic reviews have yet evaluated the effectiveness of parent-mediated play-based interventions in preschool autistic children. The overarching aims of the study will be to (i) report the key characteristics and (ii) synthesise the results of studies evaluating parent-mediated play-based interventions targeting the social communication and language skills of preschool autistic children using experimental designs. A comprehensive search for and screening of the relevant studies published between 2000 and 2021 will be undertaken. To be included, studies will have to (i) use either a randomised control trial or quasi-experimental design, (ii) focus on preschool autistic children aged six years old or younger, (iii) deliver a play-based intervention in non-educational settings, and (iv) include at least one parent as the mediator of the intervention. Data extraction of all included studies will be undertaken using a specially devised template and they will also be assessed for risk of bias using an adapted form from the Cochrane Risk of Bias tool. The overall characteristics of the included studies will be reported and a narrative synthesis of the results of the included studies will be undertaken. A meta-analysis may be performed (if justified) to report the pooled effect size of the parent-mediated play-based interventions on the social communication and language skills of preschool autistic children.

Trial registration: The current study protocol was pre-registered with the international prospective register of systematic reviews (PROSPERO: CRD42022302220).
STIMULATE-ICP-CAREINEQUAL (Symptoms, Trajectory, Inequalities and Management: Understanding Long-COVID to Address and Transform Existing Integrated Care Pathways) study protocol: Defining usual care and examining inequalities in Long Covid support
Authors: Ramasawmy Mel , Mu Yi , Clutterbuck Donna , Pantelic Marija , Lip Gregory Y. H. , van der Feltz-Cornelis Christina , Wootton Dan , Williams Nefyn H. , Montgomery Hugh , Mallinson Cookson Rita , Attree Emily , Gabbay Mark , Heightman Melissa , Alwan Nisreen A. , Banerjee Amitava , Lorgelly Paula ,   for the STIMULATE-ICP Consortium
Published: August 2022

Introduction

Individuals with Long Covid represent a new and growing patient population. In England, fewer than 90 Long Covid clinics deliver assessment and treatment informed by NICE guidelines. However, a paucity of clinical trials or longitudinal cohort studies means that the epidemiology, clinical trajectory, healthcare utilisation and effectiveness of current Long Covid care are poorly documented, and that neither evidence-based treatments nor rehabilitation strategies exist. In addition, and in part due to pre-pandemic health inequalities, access to referral and care varies, and patient experience of the Long Covid care pathways can be poor. In a mixed methods study, we therefore aim to: (1) describe the usual healthcare, outcomes and resource utilisation of individuals with Long Covid; (2) assess the extent of inequalities in access to Long Covid care, and specifically to understand Long Covid patients’ experiences of stigma and discrimination.

Methods and analysis

A mixed methods study will address our aims. Qualitative data collection from patients and health professionals will be achieved through surveys, interviews and focus group discussions, to understand their experience and document the function of clinics. A patient cohort study will provide an understanding of outcomes and costs of care. Accessible data will be further analysed to understand the nature of Long Covid, and the care received.

Ethics and dissemination

Ethical approval was obtained from South Central—Berkshire Research Ethics Committee (reference 303958). The dissemination plan will be decided by the patient and public involvement and engagement (PPIE) group members and study Co-Is, but will target 1) policy makers, and those responsible for commissioning and delivering Long Covid services, 2) patients and the public, and 3) academics.