bluuebunny/crossref_metadata_2025 huggingface dataset:
A Retrospective Study Assessing the Effect of Infections in Myocardial Infarction Patients: Mortality and Complications
Authors: Khoury Antoine El , Salameh Pascale , Hammoudeh Sarah , Mahmoud Ahmad El , Harb Tonia , Chamoun Nadia , Kalaydjian Aline , Chalhoub Lea , Meouchy Paul El , Chendeb Sara , Nasr Samer
Published: January 2022

Background: There is a well-established relationship between myocardial infarction and infection. Multiple articles describe the increased risk of myocardial infarction, both type 1 and 2, following an infectious process. However few articles have described the relation between concomitant myocardial infarction and infections on same admission mortality and complications. Methods: The aim is to assess the effect of an acquired or concomitant infection on complications and mortality during hospitalized cases of myocardial infarction. 1197 patients of different types of myocardial infarction were studied in correlation to infectious processes. Cultures from different sites were collected and isolation of various bacterial agents were studied. Mortality and various complications were compared between infected and non-infected subjects. Pearson's chi squared test was used to compare percentages (or the Fisher exact test when expected values were lower than 5). Moreover, means were compared through ANOVA, after checking data normality and homoscedasticity. A likelihood ratio backward stepwise method was used to conduct dichotomous logistic regressions, taking dichotomous outcomes as dependent variables, and sociodemographic and biological characteristics as independent variables (potential confounders).Results: Wound, sputum, blood and urine infections were associated with increased same admission mortality and complications. Microorganisms were then studied alone regardless of the site of infection and it was shown that Escherichia Coli, Escherichia Coli ESBL, Candida Albicans, Pseudomonas Aeruginosa and Staphylococcus of any type were significantly associated with same admission complications when associated with myocardial infarction. Length of stay was significantly elevated in patients with concomitant infection and it increased with the addition of positive cultures from different sites. Conclusion: Concomitant infections with myocardial infarction significantly increase the risk of same admission complications, mortality and length of stay regardless of the site of infection and type of microorganisms.
A Positive Feedback Loop Between Gastric Cancer Cells and Tumor-associated Macrophage Induces Malignancy Progression
Authors: Piao Haiyan , Fu Lingfeng , Liu Yang , Wang Yue , Meng Xiangyu , Yang Dong , Xiao Xiang , Zhang Jun
Published: January 2022

Background: Hypoxia and inflammation tumor microenvironment (TME) play a crucial role in tumor development and progression. Although increased understanding of TME contributed to gastric cancer (GC) progression and prognosis, the direct interaction between macrophage and GC cells was not fully understood.Methods: Hypoxia and normoxia macrophage microarrays of GEO database was analyzed. The peripheral blood mononuclear cell acquired from the healthy volunteers. The expression of CXCL8 in GC tissues and cell lines was detected by quantitative reverse transcription PCR (qRT-PCR), western-blot, Elisa and immunofluorescence. Cell proliferation, migration, and invasion were evaluated by cell counting kit 8 (CCK8), colony formation, real-time imaging of cell migration and transwell. Luciferase reporter assays and chromatin immunoprecipitation were used to identify the interaction between transcription factor and target gene. Especially, a series of truncated and mutation reporter genes were applied to identify precise binding sites.The corresponding functions were verified in the complementation test and in vivo animal experiment.Results: Our results revealed that Hypoxia triggered macrophage secreted C-X-C Motif Chemokine Ligand 8 (CXCL8), which induced GC invasion and proliferation. This macrophage-induced GC progression was CXCL8 activated C-X-C Motif Chemokine Receptor 1/2 (CXCR1/2) on the GC cell membrane subsequently hyperactivated Janus kinase 1/ Signal transducer and activator of transcription 1 (JAK/STAT1) signaling pathway. Then, the transcription factor STAT1 directly led to the overexpression and secretion of Interleukin 10 (IL-10). Correspondingly, IL-10 induced the M2-type polarization of macrophages through the Nuclear Factor kappa B (NF-κB) pathway-dependent mechanism and continued to increase the expression and secretion of CXCL8 through the transcription factor Nuclear Factor Kappa B Subunit 1 (NFKB1, p50). It suggested a positive feedback loop between macrophage and GC. In clinical GC samples, increased CXCL8 predicted a patient's pessimistic outcome.Conclusion: Our work identified a positive feedback loop governing cancer cells and macrophage in GC that contributed to tumor progression and patient outcome.
Decoupling of dust cloud and embedding plasma for high electron depletion in nanodusty plasmas
Authors: Petersen Andreas , Asnaz Oguz , Tadsen Benjamin , Greiner Franko
Published: January 2022

In recent years nanoparticles (nps) have become key technological products, e.g. as coatings with tunable optical gap in third generation solar cells, as nanocrystals for photonic applications, and as pharmaceutical nanocarriers. In particle sources, that use reactive, nanodusty plasmas, a high dust density changes the properties of the dusty plasma compared to a dust free plasma considerably, as the electron depletion leads to a reduced number of free electrons. This is called the Havnes effect and was central for the understanding of the famous spokes in Saturns rings. We see here, that it is also important for technological applications. Using self excited dust density waves (DDW) as a diagnostic tool, it is possible for the first time, to completely characterize an argon discharge with embedded amorphous hydrocarbon nps of different size and density. The results show, that electron depletion governs the charge of dust grains, while the size of the particles has only a weak influence. The ion density and electric potential profile are almost independent of both, dust size as well as dust density. This suggests, that the rf generated plasma and the dust cloud coexist and coupling of both is weak.
Inhibition of glycogen synthase kinase-3-beta (GSK3β) blocks nucleocapsid phosphorylation and SARS-CoV-2 replication
Authors: Shapira Tirosh , Rens Celine , Pichler Virginia , Rees William , Steiner Theodor , Jean Francois , Winkler Dirk , Sarai Iqbal , Pelech Steven , Av-Gay Yossef
Published: January 2022

GSK3β has been proposed to have an essential role in Coronaviridae infection. Screening of a targeted library of GSK3β inhibitors against SARS-CoV-2 and HCoV-229E resulted in identification of high proportion of active compounds with low toxicity to host cells. A select lead compound, T-1686568, showed dose-dependent activity against SARS-CoV-2 transcription, translation and viral particle release in multiple cell lines and primary organoids. A protein kinase substrate profiling assay combined with western blot analysis showed that SARS-CoV-2 nucleocapsid is phosphorylated by GSK3β on S180/S184, S190/S194 and T198 which have already been primed in the adjacent phospho-sites S188, T198 and S206 respectively. Inhibition by T-1686568 resulted in reduction of the S1 Spike protein levels, an accumulation of the Nucleocapsid (N) protein and maintenance of the non-structural (NSP2) level in infected Huh-7.5.1 cells, indicating that N phosphorylation might serve as a critical precursor for processing and release of mature viruses.
Ventriculostomy-Related Intracranial Hemorrhage Following Surgical and Endovascular Treatment of Ruptured Aneurysms
Authors: Lenschow Moritz , von Spreckelsen Niklas , Telentschak Sergej , Kabbasch Christoph , Goldbrunner Roland , Grau Stefan
Published: January 2022

Introduction

: Endovascular therapy of ruptured aneurysms is regularly accompanied by periprocedural heparinization and requires the use of periprocedural antiplatelets in more complex cases. This raises concerns regarding increased bleeding risks in the case of frequently required ventriculostomy. The aim of this study was to analyze risk factors for ventriculostomy-related intracranial hemorrhages (VS-ICH) in endovascular or surgical treatment of ruptured aneurysms with a focus on antithrombotic therapy.
Materials and Methods

In this retrospective analysis we included patients admitted to our institution over a 12-year period who had received at least one ventriculostomy due to subarachnoid hemorrhage-related hydrocephalus. Patients were dichotomized into an endovascular and surgical group and rates of VS-ICH were compared. Risk factors for VS-ICH were assessed in uni- and multivariate analyses.
Results

A total of 606 ventriculostomies were performed in 328 patients. Within the endovascular group, antiplatelet therapy was used in 44.8% of cases. The overall rate of ventriculostomy-related intracranial hemorrhage was 13.1%. Endovascular treatment was associated with a higher rate of VS-ICH compared to surgical treatment (p=0.011), but not in cases without antiplatelet therapy (p=0.166). Application of any antiplatelet therapy (odds ratio, 2.647 [95% confidence interval, 1.141-6.143]) and number of ventriculostomies (odds ratio, 2.513 [95% confidence interval, 1.859-3.395]) were independent predictors of ventriculostomy-related hemorrhages.
Discussion

Our findings indicate an increased risk of VS-ICH in the endovascular group if administration of antiplatelets was required. While this aspect has to be included into treatment decision making, it must be weighed against the benefits of endovascular techniques.
Three-dimensional convective dissolution of carbon dioxide into brine
Authors: Fruton Paul , Nauruzbaeva Aziza , Bataller Henri , Giraudet Cédric , Vailati Alberto , Croccolo Fabrizio
Published: January 2022

The sequestration of carbon dioxide (CO2) through storage into deep saline aquifers represents an indispensable support technology to achieve the zero-carbon target necessary to mitigate the impact of CO2 on climate change. The effectiveness of the sequestration process, partly driven by the convective dissolution of CO2 in brine, is nowadays well characterized for two-dimensional geometries, low permeabilities, and small pressures of injection of CO2. However, reliable predictions of process-efficiency are missing because of the lack of full understanding of the three-dimensional (3D) spatio-temporal behaviour of CO2-rich convective fingers in brine over a large range of injection pressures. Here, we show that the convective dissolution is determined by the instability of the boundary layer formed at the interface between the two phases and is totally independent of the overall vertical size. Experiments were conducted over a broad range of injection pressures, close to process-relevant conditions. The results show the formation of complex 3D structures, including interconnecting stream tubes at the CO2-liquid interface, which could not be detected in previous 2D Hele-Shaw studies, and fingerings. A scale-free theoretical modelling of the convective process allows us to remap our laboratory results to length-scales of relevance for geological reservoirs. The experiments and the model show that the times needed for the onset of convection and the convective flux are independent of the system size.
Experimental study of electromechanical measurement, surface analysis and corrosion inhibition of mild steel in 1M HCL by Schiff’s bases (SBs)
Authors: L Bangarappa
Published: January 2022

Potentiodynamic polarization measurements indicate that SBs acts as mixed type corrosion inhibitors. the morphology of the mild steel surface is investigated by scanning electron microscopy (SEM) and the surface composition was evaluated using energy-dispersive X-ray Spectroscopy (EDX) to show the presence of SBs on the mild steel surface in 1M HCL. The present study, three Schiff’s bases (SBs) namely 2 (2-hydroxybenzylideneamino) heptanedioic acids, 2 (4-dimethylamino benzlideamino) heptanedioic acids and 2 (4hydroxy-3-methoxybenzylideneamino) heptanedioic acids were synthesized. Using weight loss, potentiodynamic polarization and electromechanical impedance spectroscopy (EIS) techniques for corrosion inhibition properties on mild steel in 1M HCL has been investigated. The adsorption of SBs on the mild steel surface contains Langmuir adsorption isotherm. Here kinetic and thermodynamic parameters also determined to describe the mechanism of adsorption in relevance. The main object of this presentation is experimental study of the inhibiting action of synthesized Schiff’s bases of aldehydes containing nitrogen, oxygen and aromatic rings and Glutamic acid.
Comparative Evaluation of Fibrin Gel and Platelet-Rich Fibrin on Stem Cells of the Apical Papilla Fate Using a Dentin-Based Model
Authors: Sarraf Pegah , Moayeri Razieh Sadat , Shokouhinejad Noushin , Khoshkhounejad Mehrfam , Karimi Roya , Ghorbanzadeh Abdollah , Ai Jafar
Published: January 2022

Background: PRF as one of the favorable scaffolds in Regenerative Endodontic Treatment (RET), has several limitations such as the need for blood sampling and special equipment. High available commercial scaffolds such as fibrin are able to meet all the necessary requirements of dentin tissue engineering. The present study was designed to evaluate the effect of PRF and fibrin gel, with and without the presence of EDTA-treated radicular dentin segments on SCAP viability, proliferation, migration, and differentiation.Methods: Radicular dentin were prepared from extracted teeth and treated by EDTA 17% .The samples were divided into 6 groups: Dentin/PRF/Cell, Dentin/Fibrin/Cell, Dentin/Cell, PRF/Cell, Fibrin/Cell and Cell (Control). SCAP viability was assessed using MTT assay. Gene expression levels of odontogenic markers [Dentin sialophosphoprotein (DSPP), Dentin matrix protein 1(DMP1), Collagen type I Alpha 1(COL 1A1) and Alkaline phosphatase (ALP) were assessed using qrt-PCR. Cell migration were also evaluated by means of scratch test. Results: The results of MTT assay at showed that the viability of SCAP significantly increased after 7 days for both groups containing fibrin (P <0.05). The viability of SCAP seeded on Dentin/PRF and PRF significantly decreased after 7 days (P <0.001). The odontogenic markers were significantly expressed for both scaffolds in the presence of dentin segment (p<0.05). Significant decrease in scratch area was seen in Fibrin/Dentin group (p < 0.001)Conclusions:Fibrin beside EDTA-treated dentin showed great ability in survival, proliferation, differentiation, and migration of SCAP rather than PRF.
Construction of a Mutant Bacillus Subtilis Strain for High Purity Poly-γ-Glutamic Acid Production
Authors: He Linlin , Liu Lu , Ban Rui
Published: January 2022

Purpose

To construct a Bacillus subtilis strain for improved purity of poly-γ-glutamic acid.
Results

The construction of strain GH16 was achieved by knocking out five extracellular protein genes and an operon from Bacillus subtilis G423. Then we analyzed the protein content in the γ-PGA produced by the resultant strain GH16/pHPG which decreased by 6.08%. Subsequently the fla-che operon, PBSX and the yrpD, ywoF and yclQ genes were knocked out successively and the mutant strain GH17, GH18, and GH19 was obtained. Ultimately, the protein content was reduced by 43.9%. In addition, the polysaccharide content in the γ-PGA was decreased from 2.21–1.93% due to the epsA-O operon was knocked.
Conclusion

γ-PGA has potential applications as a drug carrier, sustained-releasing agent and medical composite in medicine. To our knowledge, this is the first report of engineered Bacillus subtilis strains which can produce γ-PGA with a purity higher than 97%. Our results confirmed that this upstream strategy significantly enhanced specific protein purity by the removal of extracellular protein genes in Bacillus subtilis, and it is promising in other protein purification.
SHIP-1 Regulates the Differentiation and Function of Tregs via Inhibiting mTORC1 Activity
Authors: Du Zuochen , Wang Jinzhi , Yang Di , Sun Xiaoyu , Huang Lu , Tang xinye , Du Xingrong , Shi Hao , Miller Heather , Westerberg Lisa , Akihiko Yoshimura , Liu Chaohong
Published: January 2022

Cell metabolism is crucial for orchestrating the differentiation and function of regulatory T cells (Tregs). However, the underlying signaling mechanism that coordinates cell metabolism to regulate Treg activity is not completely understood. As a pivotal molecule in lipid metabolism, the role of SHIP-1 has been studied extensively in B cells and CD4 T cells, yet its regulatory role in Tregs remains unknown. In this study, we generated “SHIP-1 KO mice” that have SHIP-1 specifically deleted in regulatory T cells by crossing Foxp3YFP-cre mice with SHIP-1fl/fl mice. Surprisingly, SHIP-1 KO mice had severe autoimmunity with increased Tregs in the thymus and disrupted peripheral T cell homeostasis. Mechanistically, CD4Cre SHIP-1flox/flox mice were found to have increased Treg precursors and SHIP-1 KO Tregs had reduced migration and stability, which caused decreased Tregs in the spleen. Additionally, the suppressive function of Tregs from SHIP-1 KO mice was diminished, along with their promotion of anti-tumor immunity. Interestingly, the PI3K-mTORC1, but not mTORC2, signaling axis was enhanced in SHIP-1 KO Tregs. In vivo treatment of SHIP-1 KO mice with rapamycin rescued the abnormal Treg percentages and peripheral T cell homeostasis, as well as Treg suppressive function. Furthermore, the treatment of wild-type mice with SHIP-1 inhibitor enhanced anti-tumor activity. Our study has revealed a previously unrecognized underlying function of SHIP-1 in Tregs, which highlights the SHIP-1-PI3K-mTORC1 axis that regulates Treg differentiation and function.
The Promoting Effects of Activated Olfactory Ensheathing Cells on Angiogenesis after Spinal Cord Injury Through the PI3K/Akt Pathway
Authors: Wang Xiaohui , Jiang Chao , Zhang Yongyuan , Chen Zhe , Fan Hong , Zhang Yuyang , Wang Zhiyuan , Tian Fang , Li Jing , Yang Hao , Hao Dingjun
Published: January 2022

ObjectiveThe aim of this study was to investigate the pro-angiogenic potential of olfactory ensheathing cells (OECs) activated by curcumin (CCM) and lipopolysaccharide (LPS) and the possible underlying mechanisms. MethodsVascular endothelial cells or tissues were cultured and treated with conditioned medium (CM) extracted from the activated through the addition of LPS and CCM or unactivated OECs. Concomitantly, the pro-angiogenic potential of OECs was assessed in vitro by aortic ring sprouting assay, endothelial wound healing assay, CCK-8 assay and tube formation assay. Subsequently, the OECs were co-cultured with endothelial cells to evaluate their promoting effect on the proliferation and migration of endothelial cells following undergoing a mechanical scratch. Moreover, the spinal cord injury (SCI) model in rats was established, and the number of endothelial cells and vascular structure in the injured area after SCI was observed with OECs transplantation. Finally, the underlying mechanism was investigated by western blot analysis of phosphorylated kinase expression with or without the MK-2206 (Akt-inhibitor). ResultThe present results showed that the activated OECs can effectively promote the proliferation, migration and vessel-like structure formation of vascular endothelial cells. Strikingly, several pro-angiogenic growth factors such as VEGF-A and PDGF-AA, which facilitate vessel formation, were found to be significantly elevated in CM. In addition, the PI3K/Akt signaling pathway involved in pro-angiogenic event caused by activated OEC CM, displaying higher phosphorylation levels in cells. On contrary, the delivery of MK2206 can effectively abrogate all the positive effects. Conclusions﻿OECs activated by LPS and CCM, have a strong pro-angiogenesis effect, and can effectively promote angiogenesis and improve the injury microenvironment when transplanted in injured spinal cord. This potentiated ability of OECs to pro-angiogenesis is likely mediated through the PI3K/Akt pathway.
Effectiveness of a long-term Tecar Therapy treatment on Knee Pain: building T.T.E.S.S.K., an evaluating scale A systematic review and meta-analysis
Authors: Sorrentino Marco , Ferrari Davide , Elena Zais Ilaria
Published: January 2022

Background

T.E.C.a.R (Transfer of Energy Capacitive and Resistive) Therapy (TT) is a form of endogenous thermotherapy based on radio waves 1 . It is a non-invasive procedure, that makes use of a machine able to stimulate heat inside the body to repair the inflammatory processes. Although being well known in the physiotherapy and massage therapy ambient for its benefic effects, which are still not very explored especially physiological and biochemical ones; TT is commonly used by therapists for musculoskeletal diseases. Anyhow, TT is often used as a palliative remedy for musculoskeletal disease, or linked with an ibuprofen or antidolorific medical therapy, and not as a main cure. Current literature still lacks objective ways to evaluate TT treatment. This study aims to create an evaluating Scale for the knee joint lesions treated with TT, for athletes or sports enthusiasts.

Methods

PubMed, Embase, and Cochrane Library databases were screened to perform an extensive review. PRISMA guidelines were applied, and the risk of bias was assessed, as was the methodological quality of the included studies. 25 articles were reviewed. 178 patients (total amount of clinic cases in PubMed, Embase and Cochrane Library articles combined) have decreased their pain after a long-term TT treatment (> 3 or 5 sessions).

Results

Knee joint is often affected by non-sportive or sportive musculoskeletal lesions. ((At this point, not only the TT articles were reviewed, but also all the articles (122) that included these key aspects)). Six items were selected to evaluate the efficacy of TT on the impaired joint: Range of Motion (ROM), %MVIC of quadriceps, biceps femoris and gastrocnemius, Cincinnati Rating Scale System (CRSS), NRS Pain Scale, Muscle Fiber Orientation (MFO) and %Trigger Points.

Conclusions

Our study allows to build a new treatment methodology with TT. It guarantees the therapist to structure a long-lasting follow-up, which is not limited to the simple use of the machine. Applying this methodology, the patient receives a treatment that is not limited, but built on his own needs thanks to the distribution of the summing scores, strictly dependent on the type of clinical case that the therapist finds. Thanks to the six items we inserted in T.T.E.S.S.K. (“Tecar Therapy Evaluation Score System of the Knee”), every aspect of a musculotendinous pathology is recognized, and might be treated with TT by therapists.
Reliability of Shear Wave Elastography for the Assessment of Gastrocnemius Fascia Elasticity in Healthy Individual
Authors: Zhou Jiping , Lin Yuyi , Zhang Jiehong , Si’tu Xingxian , Wang Ji , Pan Weiyi , Wang Yulong
Published: January 2022

The mechanical properties of deep fascia (i.e. an index of stiffness) strongly affect the development of muscle pathologies, and muscular actions, such as compartment syndromes. Actually, a clear understanding of the mechanical characterization of muscle deep fascia still lacks. The present study focuses on examining the reliability of ultrasonic shear wave elastography device (USWE) in quantifying the shear modulus of gastrocnemius fascia in healthy individual and the device’s abilities to examine the shear modulus of gastrocnemius deep fascia during ankle dorsiflexion. Twenty-one healthy males participated in the study (age: 21.48±1.17 years). The shear modulus of the medial gastrocnemius fascia (MGF) and lateral gastrocnemius fascia (LGF) were quantified at different angles using USWE during passive lengthening. The operators took turns to measure each subject’s MGF and LGF over 1-hour period and by operator B with a 2-hour interval. In the intra-operator test, the same subjects participated at the same time 5 days later. The intra-rater [ Intra-class correlation coefficient (ICC) = 0.846-0.965)] and inter-rater (ICC = 0.877-0.961) reliabilities for measuring the shear modulus of the MGF and LGF were rated as both excellent, and the standard error in measurement (SEM) was 3.49 kPa, the minimal detectable change (MDC) was 9.68 kPa. Regardless of the ankle angle, the shear modulus of the LGF were significant greater than that of the MGF (p < 0.001). The significant increase in the shear modulus both of the MGF and LGF were observed at neutral position compared to the relaxed position. This results indicate that the USWE is a technique to assess the shear modulus of gastrocnemius fascia and detect its dynamic changes during ankle dorsiflexion. USWE can be used for biomechanical study and intervention experiments of deep fascia.
Apigenin Induces Browning in White Adipocytes Mediated by VEGF-PRDM16 Signaling.
Authors: Sreeku Sreelekshmi , Vijayan Vinu , Singh Fathe , Sudhakar Manu , S Kiran M
Published: January 2022

The white adipose tissues are metabolically inert which results in deranged biological signalling disorders resulting in obesity. Lack of vascularisation in these tissues is mainly responsible to make them metabolically inert. Not much work has been done in this direction to understand the role of angiogenesis in white adipocytes metabolism. In the present study, we evaluated the effect of angiogenic modulator in modulating the metabolism in white adipocyte. Nutraceuticals apigenin (Apg) was employed as angiogenic modulator. The results indicated that promoting angiogenesis by Apg enhanced the de novo differentiation and trans-differentiation of white adipocyte into brown like phenotype by triggering vascular endothelial growth factor A. Cross talk between endothelial and adipocytes were observed in co-culture studies. The metabolic shift in white adipocytes was observed to be due to the upregulation of PRDM16 cascade. The study provides new insights for inducing metabolic shift in white adipocytes by modulation of angiogenesis in white adipocyte to trigger browning for the treatment of obesity. Further the study opens scopes for development of medical devices for obese subjects, an area that needs to be addressed specifically with reference to soft tissue engineering as commercial soft tissue engineering scaffolds does not suit the obese patients.
The Diagnostic and Prognostic Value of BEN-Domain (BEND) Family Genes in Gastric Cancer
Authors: Fan Jiaxin , Yang Min , Liang Chaojie , Liang Chaowei , Guo Jiansheng
Published: January 2022

BEND(BEN domain-containing protein)is a domain protein-coding gene, whose abnormal expression is related to the occurrence of malignant tumors. But studies on gastric cancer are rare. We attempted to investigate the role of BEND family genes in evaluating the prognosis of gastric cancer and guiding clinical treatment. We analyzed the BEND family genes expression, prognostic value, and drug sensitivity in pan-cancer, and the correlation between their expression and tumor microenvironment of gastric cancer, stemness index, immune subtypes, and clinicopathological characteristics were analyzed. We constructed a model using BEND3P1 and BEND6 to evaluate the prognosis of gastric cancer patients. Multivariate Cox proportional risk model analysis showed that risk score is an independent risk factor for gastric cancer patients. To assess the value of risk score for prognosis, patients were divided into high-risk and low-risk groups based on median risk scores, and survival analyses were performed. The results showed that the OS of patients with high-risk scores is significantly lower. We also constructed a nomogram to predict individual survival probability using the BEND risk score and clinical case characteristics. In conclusion, the BEND family genes can predict the prognosis and guide the treatment of gastric cancer patients.
Bmi1 Promotes Cholangiocarcinoma Progression And Correlates With Antitumor Immunity In An Exosome-Dependent Manner
Authors: Liu Zengli , Hu Chunxiao , Zheng Lijie , Liu Jialiang , Li Kangshuai , Li Xingyong , Wang Yue , Mu Wentao , Chen Tianli , Shi Anda , Qiu Bo , Zhang Xin , Zhang Zongli
Published: January 2022

Background: Cholangiocarcinoma (CCA) is a class of malignant tumors originating from bile duct epithelial cells. Due to difficult early diagnosis and limited treatment, the prognosis of CCA is extremely poor. Bmi1 is dysregulated in many human malignancies. However, the prognostic significance and oncogenic role of Bmi1 in cholangiocarcinoma (CCA) are not well elucidated. Methods: In the present study, we investigated its clinical importance and the potential mechanisms in the progression of CCA. We detected Bmi1 expression in a large CCA cohort. We demonstrated Bmi1 was substantially upregulated in CCA tissues and was identified as an independent prognostic biomarker of CCA. Moreover, overexpression of Bmi1 promoted CCA proliferation, migration, and invasion. And, Bmi1 knockdown could inhibit proliferation and metastases of CCA in vitro and in vitro/vivo validation. Interestingly, we found that CCA-derived exosomes contain Bmi1 proteins, which can transfer Bmi1 between CCA cells. The unique Bmi1-containing exosomes promote CCA proliferation and metastasis through autocrine/paracrine mechanisms. In addition, we demonstrated that Bmi1 inhibits CD8+T cell-recruiting chemokines by promoting repressive H2A ubiquitination in CCA cells. Conclusions: Bmi1 is an unfavorable prognostic biomarker of CCA. Our data demonstrate a novel function of Bmi1 in CCA tumorigenesis and metastasis mediated by exosomes. Besides, Bmi1 inhibition may augment immune checkpoint blockade to inhibit tumor progression by activating cell-intrinsic immunity of CCA
Psychometric Properties of the Emotional Processing Scale in Individuals with Psychiatric Symptoms and the Development of a Brief 15-Item Version
Authors: Maroti Daniel , Axelsson Erland , Ljótsson Brjánn , Andersson Gerhard , Lumley Mark , Johansson Robert
Published: January 2022

Objective

The 25-item Emotional Processing Scale (EPS) is a measure of emotional processing. There is a scarcity of research about its factor structure, test-retest reliability, and validity in individuals with psychiatric symptoms.
Method

We administered the EPS-25 to a sample (N=512) of people with elevated psychiatric symptoms, using confirmatory factor analysis to evaluate three a priori models from previous research. We then evaluated discriminant and convergent validity against measures of alexithymia (Toronto Alexithymia Scale-20; TAS-20), depressive symptoms (Patient Health Questionaire-9; PHQ-9) and anxiety symptoms (Generalized Anxiety Disorder-7; GAD-7).
Results

None of the a priori models achieved acceptable fit, and subsequent exploratory factor analysis did not lead to a convincing factor solution for the 25 items. A 5-factor model did, however, achieve acceptable fit if we retained only 15 items (EPS-15) and this solution could be replicated in a holdout sample. Convergent and discriminant validity for EPS-15 was r=-.19-.46 vs. TAS-20, r=.07-.25 vs. PHQ-9, r=.29-.57 vs. GAD-7. Test-retest reliability was ICC=.73.
Conclusions

This study strengthens the case for the reliability and validity of the five factor Emotional Processing Scale but suggest that only 15 items should be retained. Future studies should further examine the reliability and validity of the EPS-15.
Industry-Oriented Method for Dynamic Force Identification in Peripheral Milling Based on FSC-LSQR Using Acceleration Signals
Authors: Hou Maxiao , Cao Hongrui , Li Qi , Shi Jianghai
Published: January 2022

Online measurement of milling force play a vital role in enabling machining process monitoring and control. In practice, the milling force is difficult to be measured directly with the dynamometer. This paper develops a novel method for milling force identification called least square QR-factorization with fast stopping criterion (FSC-LSQR) method, and the queue buffer structure (QBS) is employed for the online identification of milling force using acceleration signals. The convolution integral of milling force and acceleration signals is discretized, which turns the problem of milling force identification into a linear discrete ill-posed problem. The FSC-LSQR algorithm is adopted for milling force identification because of its high efficiency and accuracy, which handles the linear discrete ill-posed problem effectively. The online identification of milling force can be realized using the acceleration signal enqueue and the milling force dequeue operations of the QBS. Finally, the effectiveness of the method is verified by experiments. The experimental results show that the FSC-LSQR algorithm running time is within \((0.05s)\) and the calculation error is less than \((10\%)\). The proposed method can make the sampling frequency of the milling force reach 10240Hz by employing QBS, which satisfy the industry requirements of milling force measurement.
Dexmedetomidine Versus Propofol Sedation in Flexible Bronchoscopy: A Randomized Controlled Trial and a Systematic Review and Meta-Analysis
Authors: Pertzov Barak , Krasulya Boris , Azem Karam , Shostak Yael , Izhakian Shimon , Rosengarten Dror , Khaershenko Svetlana , Kramer Mordechai Reuven
Published: January 2022

Background:Dexmedetomidine (DEX), is a highly selective alpha2 adrenoceptor (α2-AR) agonist, successfully used in various procedures including flexible bronchoscopy. Randomized controlled trials (RCTs) evaluating DEX sedation during bronchoscopy report equivocal results regarding respiratory and hemodynamic outcomes. Methods We conducted an RCT to evaluate the efficacy of dexmedetomidine compared to propofol for sedation during bronchoscopy. The primary outcome was desaturation events, secondary outcomes were transcutaneous Pco2 level, hemodynamic adverse events and physician and patient satisfaction. We have also conducted A systematic review and meta-analysis of all RCTs evaluating DEX sedation during flexible bronchoscopy, included current study results.ResultsOverall, 63 patients were included, 30 and 33 in the DEX and propofol groups, respectively. The number of desaturation events was similar between groups, median (IQR) 1 (0-1) and 1 (0-2) in the DEX and control groups, respectively (P=0.29). Median desaturation time was 1 (0-2) and 1 (0-3) minutes in the DEX and control groups, respectively (P=0.48). Adverse events included hypotension, 33% vs 21.1% in intervention and control groups, respectively (P=0.04), bradycardia, cough, and delayed recovery from sedation. Total adverse events were 22 and 7 in DEX and propofol groups, respectively (P=0.009). The pooled meta-analysis included 13 trials (1604 participants) showed a significantly lower rate of desaturation events in the DEX group (RR 0.67, 95% CI 0.57 to 0.79) with a significantly higher rate of hypotension and bradycardia events (RR 1.55, 95% CI 1.16 to 2.06 and RR 1.91, 95% CI 1.04 to 3.5, respectively)ConclusionDexmedetomidine sedation resulted in a significantly reduced rate of desaturation events in comparison to propofol, midazolam and fentanyl. However, it was also associated with a higher rate of hypotension and bradycardia.Trial registration : NCT04211298, registration date: 26/12/2019
Farm Parcel Extraction in High Resolution Remote Sensing Image Based on Hierarchical Spectrum and Shape Features
Authors: Li Bangyu
Published: January 2022

Background: Land-use classification schemes typically address both land use and land cover. Vectorized data extracted from farm parcel segmentation provides important cadastral data for the formulation and management of climate change policies. It also provides important basic data for research on pest control in large areas, crop yield forecasts, and crop varieties classification. It can also be used for the assessment of compensation for damages related to extreme weather events by the agricultural insurance department. Firstly, we investigate the effectiveness of an automated image segmentation method based on TransUNet architecture to enable that automate the task of farm parcel delineation that originally relied on high labor costs. Then, post-processing by vectoring binary segmentation image, which the area and regularity parameter to adjust the accuracy of segmentation, can get a more optimized image segmentation result.Results: The results on the existing data show that the automatic segmentation system we proposed is a method that can effectively divide various types of agricultural land. The system was trained and evaluated using 94780 images. The performance parameters obtained showed that the accuracy rate reached 83.31%, the recall rate reached 82.13%, the F1-S rate was 80.37%, the total accuracy rate was 82.23%, and Iou was 80.39%. At the same times, without losing too much accuracy, we train and test the model with 3m resolution image, which has the advantage of processing speed than 0.8m resolution. Therefore, our proposed method can be effectively applied to the task of extraction of agricultural land, which is better and more efficient than most manual annotations.Conclusions: We have demonstrated the effectiveness of strategy using a TransUNet architecture and postprocessing by vectoring binary segmentation for farm parcel extraction in high remote sensing images. The success of our approach is also a demonstration of feasibility of the deep learning to participate in and improve agricultural production activities, which is important for achieving scientific management of agricultural production.
Phenylalanine Suppresses Cell Death Caused by Loss of Fumarylacetoacetate Hydrolase in Arabidopsis
Authors: Jiang Yihe , Zhu Qi , Yang Hua , Zhi Tiantian , Ren Chunmei
Published: January 2022

Fumarylacetoacetate hydrolase (FAH) catalyzes the final step of Tyrosine (Tyr) degradation pathway essential to animals and the deficiency of FAH causes an inborn lethal disease. In plants, a role of this pathway was unknown until we found that mutation of Short-day Sensitive Cell Death1 (SSCD1), encoding Arabidopsis FAH, results in cell death under short day. Phenylalanine (Phe) could be converted to Tyr and then degraded in both animals and plants. Phe ingestion in animals worsens the disease caused by FAH defect. However, in this study we found that Phe represses cell death caused by FAH defect in plants. Phe treatment promoted chlorophyll biosynthesis and suppressed the up‑regulation of reactive oxygen species marker genes in the sscd1 mutant. Furthermore, the repression of sscd1 cell death by Phe could be reduced by α-aminooxi-β-phenylpropionic acid but increased by methyl jasmonate, which inhibits or activates Phe ammonia-lyase catalyzing the first step of phenylpropanoid pathway, respectively. In addition, we found that jasmonate signaling up‑regulates Phe ammonia-lyase 1 and mediates the methyl jasmonate enhanced repression of sscd1 cell death by Phe. These results uncovered the relation between chlorophyll biosynthesis, phenylpropanoid pathway and jasmonate signaling in regulating the cell death resulting from loss of FAH in plants.
Gene expression and DNA methylation altering lead to the high oil content in wild allotetraploid peanut (A. monticola)
Authors: Liu Nian , Pandey Manish , Wu Bei , Huang Li , Luo Huaiyong , Chen Yuning , Zhou Xiaojing , Chen Weigang , Huai Dongxin , Lei Yong , Liao Boshou , Varshney Rajeev , Jiang Huifang
Published: January 2022

The wild allotetraploid peanut Arachis monticola contains higher oil content than cultivated allotetraploid Arachis hypogaea. To investigate its molecular mechanism controlling oil accumulation, we performed comparative transcriptomics from developing seeds between three Arachis monticola and five Arachis hypogaea varieties. The analysis not only showed species-specific grouping based on transcriptional profiles but also detected two gene clusters with divergent expression patterns enriched in lipid metabolism. Further, the differential expression gene analysis also indicated expression alteration in wild peanut leading to enhanced activity of oil biogenesis and limiting the rate of lipid degradation. We also constructed a regulatory network of lipid metabolic DEGs with co-expressed transcription factors. In addition, bisulfite sequencing was conducted to characterize the variation of DNA methylation between wild allotetraploid (245, WH 10025) and cultivated allotetraploid (Z16, Zhh 7720) genotypes. Genome-wide DNA methylation was found antagonistically correlated with gene expression during seed development. The results indicated that CG and CHG contexts methylation may negatively regulate specific lipid metabolic genes and transcription factors to subtly affect the difference of oil accumulation. Our work provided the first glimpse on the regulatory mechanism of gene expression altering for oil accumulation in wild peanut and gene resources for future breeding applications.
A Novel Approach of Tracing in Vivo Bioluminescence Imaging Expression of Vitrified Immature Testicular Tissue Grafts Until Adulthood: A Translational Transgenic Mouse Model
Authors: Lu Buo-Jia , Liu Yung-Liang , Lin Bou-Zenn , Chen Chi-Huang
Published: January 2022

Background: The optimal method for cryopreserving immature testicular tissue (ITT) remains unknown and there is no standardized protocol. Controlled slow freezing remains the mainstream method of choice in human prepubertal male fertility preservation. Currently, the outcomes for ITT vitrification are conflicting, and most data are limited to in vitro animal studies.Methods: A total of 12 pairs of donor and recipient mice were included in our experiments. The donors were immature transgenic mice, and the recipients were wild-type male mice. In the vitrification group, ITT was vitrified and thawed before transplantation. In the control group, ITT was transplanted to the recipients immediately. After thawing, we measured the expression of apoptosis-related mRNA caspase-3. More importantly, we monitored to adulthood all the transplanted grafts in vivo using noninvasive bioluminescence imaging (BLI) technology. On day 31, we removed the grafts for evaluation via hematoxylin and eosin staining and immunohistochemistry (IHC).Results: We traced the survival of the grafts by in vivo BLI on days 1, 2, 5, 7, and 31 after transplantation. In both the vitrification and the control groups, bioluminescence decreased between days 2 and 5. Subsequently, the bioluminescence showed an upward trend until day 31. Compared with day 1, the bioluminescence was significantly stronger on day 31 after transplantation (P = 0.009). The differences between the two groups were constantly insignificant after analysis. These results indicate that both fresh and frozen–thawed testicular tissues can survive for at least 31 days after transplantation. Moreover, the vitrification group showed BLI signals comparable with those of fresh tissues. Compared with the control group, expression of the caspase-3 gene was significantly increased after vitrification (P = 0.04). Histology and IHC showed that both tissue structure and protein expression were intact in both groups.Conclusions: Transplanted vitrified ITT grafts could survive till adulthood with BLI intensity comparable to that of the fresh control. Intact cells and structures for spermatogenesis in vitrified ITT grafts were as well-preserved as those in the control group. This translational model of self-repairing vitrified ITT grafts in vivo, lends weight to the role of vitrification in prepubertal male fertility preservation.
Optimal Solution of Neutrosophic Linear Fractional Programming Problems With Mixed Constraints
Authors: DAS SAPAN , Edalatpanah S A
Published: January 2022

In this paper, Linear Fractional Programming (LFP) problems have been extended to neutrosophic sets (NSs) and the operations and functionality of these laws are studied. Moreover, the new algorithm is based on aggregation ranking function and arithmetic operations of triangular neutrosophic sets (TNSs). Furthermore, for the first time, in this paper, we take up a problem where the constraints are both equality and inequality neutrosophic triangular fuzzy number. Lead from genuine issue, a few numerical models are considered to survey the legitimacy, profitability and materialness of our technique. At last, some numerical trials alongside one contextual analysis are given to show the novel techniques are better than the current strategies.
Identification of Different Proteins Binding to Na, K-ATPase α1 in LPS-Induced ARDS Cell Model by Proteomic Analysis
Authors: Wen Xu-Peng , Long Guo , Zhang Yue-Zhong , Huang He , Liu Tao-Hua , Wan Qi-Quan
Published: January 2022

Background: Acute respiratory distress syndrome (ARDS) is characterized by refractory hypoxemia caused by accumulation of pulmonary fluid, which is related to inflammatory cell infiltration, impaired tight junction of pulmonary epithelium and impaired Na, K-ATPase function, especially Na, K-ATPase α1 subunit. Up until now, the pathogenic mechanism at the level of protein during lipopolysaccharide- (LPS-) induced ARDS remains unclear.Methods: Using an unbiased, discovery and quantitative proteomic approach, we discovered the differentially expressed proteins binding to Na, K-ATPase α1 between LPS-A549 cells and Control-A549 cells. These Na, K-ATPase α1 interacting proteins were screened by co-immunoprecipitation (Co-IP) technology. Among them, some of the differentially expressed proteins with significant performance were identified and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The protein interaction network was constructed by the related Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Several differentially expressed proteins were validated by Western blot.Results: Of identified 1598 proteins, 89 were differentially expressed proteins between LPS-A549 cells and Control-A549 cells. Intriguingly, protein-protein interaction network showed that there were 244 significantly enriched co-expression among 60 proteins in the group control-A549. while the group LPS-A549 showed 43 significant enriched interactions among 29 proteins. The related GO and KEGG analysis found evident phenomena of ubiquitination and deubiquitination, as well as the pathways related to autophagy. Among proteins with rich abundance, there were several intriguing ones, including the deubiquitinase (OTUB1), the tight junction protein zonula occludens-1 (ZO-1), the scaffold protein in CUL4B-RING ubiquitin ligase (CRL4B) complexes (CUL4B) and the autophagy-related protein sequestosome-1 (SQSTM1).Conclusions: In conclusion, our proteomic approach revealed targets related to the occurrence and development of ARDS, being the first study to investigate significant differences in Na, K-ATPase α1 interacting proteins between LPS-induced ARDS cell model and control-A549 cell. These proteins may help the clinical diagnosis and facilitate the personalized treatment of ARDS.