bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic Changes in Physicochemical Properties and Microbial Diversity During the Fermentation of Mao-Tofu
Authors: Li Dongqi , Wan Yaqiong , Zhou Xiaohan , Cheng Juanjuan , Zhang Jieping , Cheng Jianghua , Xu Yayuan
Published: February 2025

Mao-tofu is famous for its unique flavour and texture in Anhui Province, China. The physicochemical properties and microbial diversity of Mao-tofu during different fermentation periods were studied. The pH of the tofu was acidic, the moisture gradually decreased, and the hardness, viscosity, and chewiness gradually increased, while the elasticity gradually decreased. Among these, changes in volatiles and synergistic effects of proteins, peptides, and free amino acids (FAAs) affect the flavour of Mao-tofu. Histamine had the highest concentration among all biogenic amine (BA) during the whole fermentation process. A microbial community analysis showed that Lactobacillus and Trichosporon were the most important strains throughout the fermentation process. Bacterial abundance and diversity also showed a gradual increase, while fungal abundance and diversity showed a gradual decrease. A comprehensive analysis of the physicochemical changes associated with microbial succession can help to gain insights into the maturation process of texture and flavour during the production of Mao-tofu.
Characterization of Extruded Sorghum-Soy Blends to Develop Pre-Cooked and Nutritionally Dense Fortified Blended Foods
Authors: Joseph Michael , Guo Qingbin , Lindshield Brian , Adedeji Akinbode A. , Alavi Sajid
Published: February 2025

Food aid commodities are essential food items in global food aid programming. Some are primarily made from an extrusion of corn and soybeans. However, there are concerns about the genetically modified organisms (GMOs) of some of these grains. Hence, there is a need for alternatives to grains, like sorghum, which is not GMO. It is critical to ensure that products from this new ingredient meet the quality requirements, hence the need to profile them. An expanded formulation sorghum-soy blend (SSB), obtained from extrusion cooking, was ground using a hammer mill and analyzed for changes in properties that were affected by the transformation of starch and protein during processing. Macro- and micro-nutrients were added to these milled blends to prepare fortified blended foods (FBFs) that could meet the recommendations of Food Aid Quality Review (FAQR) report on energy, protein, and micronutrient content. The water absorption index (WAI) ranged from 2.82 to 5.90 g/g, the water solubility index (WSI) ranged from 6.22 to 18.50%, and the blends were affected by the formulation—whole/decorticated sorghum and different levels of fat. Extrusion processing caused starch gelatinization in the range of 90.69–96.26%. The pasting properties indicated that whole grain blends of SSB had lower peak time and higher final viscosity when compared to decorticated sorghum blends. The Bostwick flow rate of cooked porridges with 20% solids was within the recommended range of 9–21 cm/min. Starch digestibility significantly increased after extrusion, with a 149.65% increase in rapidly digestible starch (RDS). The protein digestibility did not vary significantly when subjected to extrusion and wet cooking. There was a significant reduction in anti-nutritional factors in the extruded binary blends of SSB when compared to respective raw blends: phytic acid was reduced by 25.33%, tannins were not found, and trypsin inhibitors were reduced by 19.50%. Thus, the extrusion processing of SSB with the subsequent addition of macro- and micro-ingredients was effective in producing FBFs with high nutritive value, comparable to FBF made from traditional ingredients.
Progress on Fractal Dimensions of the Weierstrass Function and Weierstrass-Type Functions
Authors: Qiu Yue , Liang Yongshun
Published: February 2025

The Weierstrass function W(x)=∑n=1∞ancos(2πbnx) is a function that is continuous everywhere and differentiable nowhere. There are many investigations on fractal dimensions of the Weierstrass function, and the investigation of its Hausdorff dimension is still ongoing. In this paper, we summarize past researchers’ investigations on fractal dimensions of the Weierstrass function graph.
Gamma-Ray Bursts: The Energy Monsters of the Universe
Authors: Giovannelli Franco
Published: February 2025

Gamma-Ray Bursts(GRBs) are the most violent and energetic astrophysical phenomena, which I dare call “the Energy Monsters of the Universe”. Indeed, they show an enormous emitted isotropic energy ranging from ∼3 × 1046 erg (GRB 170817A) to ∼1055 erg (GRB 221009A) and a duration ranging from ≈milliseconds to ∼104 s. In this review—which I agreed to write as a scientist not directly involved in the field of GRBs—I will present the history of GRBs from the time of their discovery by chance until the new era whose beginning was marked by the detection of gravitational waves coming from the merger of two neutron stars. I will discuss the experimental results and their physical interpretation, which is still a source of heated debate within the scientific community. Due to the reasonable length of this review and especially given my limited knowledge, I do not claim to have exhausted the complicated topic of GRBs, but to have contributed in making this subject easy to read for non-experts, providing a critical contribution that is hopefully useful to the whole community.
Transcriptome Analysis of Environmental Adaptation of Largemouth Bass (Micropterus salmonides)
Authors: Wang Yuao , Li Huan , Li Chuan , Tang Weibin , Wang Yanchao , Hou Hongxia
Published: February 2025

Background/Objectives: The largemouth bass (Micropterus salmonides) is a farmed fish of significant economic value, and studying its adaptability is crucial for enhancing the economic benefits of aquaculture. The largemouth bass changes gene expression pattern to rapidly adapt to environmental changes and maintain normal physiological function. Methods: In this study, largemouth bass from two distinct environmental backgrounds—Huzhou and Xingtai—were used as experimental materials, and they have significantly different breeding conditions. Comparative transcriptomics was used to analyze the gene expression patterns in largemouth bass from both backgrounds. Results: In the female, there were 1678 differentially expressed genes, of which 541 were upregulated and 1137 were downregulated. Meanwhile, in the male, there were 1287 differentially expressed genes, including 542 upregulated genes and 745 downregulated genes. The differentially expressed genes were mainly enriched in biological processes such as metabolic process, biological regulation, response to stimulus, developmental process, signaling, reproduction and immune system process. The enriched pathways included carbon metabolism, glycolysis/gluconeogenesis, purine metabolism, biosynthesis of amino acids, starch and sucrose metabolism, fructose and mannose metabolism, pyrimidine metabolism, MAPK signaling pathway, spliceosome, protein processing in the endoplasmic reticulum, ribosome biogenesis in eukaryotes, etc. Conclusions: We speculated that largemouth bass in Xingtai may adapt to the environment by downregulating metabolism- and reproduction-related genes and altering the expression of immune-related genes. Our study provided molecular evidence for the adaptation research of largemouth bass and provided a scientific basis for optimizing largemouth bass breeding technology.
MicroRNAs in the Mitochondria–Telomere Axis: Novel Insights into Cancer Development and Potential Therapeutic Targets
Authors: Cruz-Ramos José Alfonso , de la Mora-Jiménez Emmanuel , Llanes-Cervantes Beatriz Alejandra , Damián-Mejía Miguel Ángel
Published: February 2025

The mitochondria–telomere axis is recognized as an important factor in the processes of metabolism, aging and oncogenesis. MicroRNAs (miRNAs) play an essential function in this complex interaction, having an impact on aspects such as cellular homeostasis, oxidative responses and apoptosis. In recent years, miRNAs have been found to be crucial for telomeric stability, as well as for mitochondrial behavior, factors that influence cell proliferation and viability. Furthermore, mitochondrial miRNAs (mitomiRs) are associated with gene expression and the activity of the cGAS/STING pathway activity, linking mitochondrial DNA recognition to immune system responses. Hence, miRNAs maintain a link to mitochondrial biogenesis, metabolic changes in cancer and cellular organelles. This review focuses on the roles of a variety of miRNAs in cancer progression and their potential application as biomarkers or therapeutic agents.
Exploring the Role of IRF6 in Perinatal Arterial Ischemic Stroke: A Case of a Newborn with Craniofacial Malformations
Authors: Perilli Lorenzo , Negro Simona , Carbone Samanta , Minerva Michele , Curcio Maria Rosaria , Lotti Federica , Mencarelli Maria Antonietta , Ariani Francesca , Renieri Alessandra , Tomasini Barbara , Grosso Salvatore
Published: February 2025

Background/Objectives: Ischemic arterial stroke (AIS) is a cerebrovascular event that can occur acutely within the first hours or days of life, presenting as a neurological emergency. To date, clearly defined genetic risk factors for AIS have not been established, although certain genes involved in cerebrovascular regulation mechanisms are suspected to play a role. The Interferon Regulatory Factor 6 (IRF6) gene is a transcription factor involved in craniofacial and epidermal development. Recently, pathogenic variants of IRF6 have been implicated in the cytoprotective pathway of ischemic cerebrovascular disease. The aim of this manuscript is to further support the already-reported association between IRF6 and AIS. Materials and Methods: Genetic counseling and exome sequencing analysis were conducted for diagnostic purposes. Results: We report the case of a female newborn with palatoschisis, cleft palate, sensorineural deafness, facial dysmorphisms, and cutaneous defects who suffered an ischemic stroke in the territory of the left middle cerebral artery on day 1 of life. Family and pregnancy histories revealed no identifiable risk factors, and coagulation studies were normal. Exome sequencing identified a de novo c.1124T>C (p.Phe375Ser) variant in the IRF6 gene. The child developed right spastic hemiplegia and began motor rehabilitation therapy. Recently, a genome-wide association study (GWAS) using m6A-SNPs identified a statistical association between AIS and a single nucleotide polymorphism (SNP) that influences the expression of the IRF6 gene as an expression quantitative trait locus (eQTL). Conclusions: To our knowledge, this is the first report of neonatal ischemic stroke in a child carrying a de novo IRF6 pathogenic variant, further supporting its potential role as a genetic factor influencing cerebrovascular events. Further studies are needed to elucidate the precise relationship between IRF6 and AIS.
An Exploratory Genomic and Transcriptomic Analysis Between Choloepus didactylus and Homo sapiens
Authors: Baran Ariella , Ibrahim Antony , Nakano Yuka , Aoshima Hideyuki , Ozeki Takeshi , Sato-Baran Iri , Ordinario David D.
Published: February 2025

Background/Objectives: Sloths, a group of xenarthran mammals currently comprising six recognized distinct species, have been the focus of much physiological animal research due to their extremely slow metabolisms, deliberate movements, and their status as a species relatively unchanged for over 26 million years. However, despite all the effort aimed at understanding these unique characteristics, the sloth genome remains largely unexplored. Due to the link between genetics and observed traits, such an investigation could potentially lead to insights regarding the genetic basis of unique sloth behaviors and characteristics, such as slow movement, low metabolism, and longevity. Methods: In this exploratory investigation, we performed whole genomic and transcriptomic analysis of a female Choloepus didactylus (Linnaeus’s Two-Toed Sloth). Through whole genome sequencing (WGS), the genetic overlap between female two-toed sloths and female humans was estimated in line with evolutionary biology. Results: Transcriptome analysis of peripheral blood mononuclear cells (PBMCs) showed significant differences between gene expression levels in two-toed sloths and humans related to metabolism, body temperature control, cell cycle regulation, telomere maintenance, circadian rhythm regulation, and cancer prevention. Conclusions: The discovered differences imply a relationship to the low metabolisms, slow movements, and longevity displayed by sloths. Future exploratory research will include additional testing to determine if these findings are universal among all recognized sloth species, as well as to address the relationship between specific gene and protein functions and observed traits.
Sustained Epigenetic Reactivation in Fragile X Neurons with an RNA-Binding Small Molecule
Authors: Kam Christina W. , Dumelie Jason G. , Ciceri Gabriele , Yang Wang-Yong , Disney Matthew D. , Studer Lorenz , Jaffrey Samie R.
Published: February 2025

Background/Objectives: Fragile X syndrome (FXS) is a disease of pathologic epigenetic silencing induced by RNA. In FXS, an expanded CGG repeat tract in the FMR1 gene induces epigenetic silencing during embryogenesis. FMR1 silencing can be reversed with 5-aza-deoxyctidine (5-aza-dC), a nonspecific epigenetic reactivator; however, continuous administration of 5-aza-dC is problematic due to its toxicity. We describe an approach to restore FMR1 expression in FXS neurons by transient treatment with 5-aza-dC, followed by treatment with 2HE-5NMe, which binds the CGG repeat expansion in the FMR1 mRNA and could block the resilencing of the FMR1 gene after withdrawal of 5-aza-dC. Methods: This study uses immunofluorescence and fluorescent in situ hybridization (FISH) to measure whether FMR1 expression is maintained in FXS post-mitotic neurons treated with 2HE-5NMe. Genome-wide profiling of histone marks was used to monitor epigenetic changes and drug selectivity in response to 5-aza-dC followed by 2HE-5NMe treatment. Changes to dendritic morphology were visualized using confocal microscopy. Results: In this study, we find that 2HE-5Nme maintains FMR1 in a reactivated state after reactivation using 5-aza-dC in post-mitotic neurons. FMR1 reactivation in neurons results in the re-expression of FMRP and reversal of FXS-associated dendritic spine defects. Conclusions: These results demonstrate that an RNA-binding small molecule can achieve gene-specific epigenetic control and provide an approach for the restoration of FMRP in FXS neurons.
Plate Tectonics and Metamorphism: Teaching Complex Systems Using Videos and Animations
Authors: Willis Siloa , Stern Robert J.
Published: February 2025

Metamorphism is a complex geologic process that is often poorly covered in introductory geology courses. This study explores the effectiveness of a video-based instructional intervention in improving student understanding of metamorphism and its relationship to plate tectonics. The intervention includes an innovative assessment procedure featuring embedded QR codes, allowing participants to complete pre- and post-tests seamlessly. Data were collected from 75 participants, with results showing modest to major improvements in conceptual understanding, particularly about geothermal gradients. However, minimal improvement was observed in questions requiring deeper knowledge of specific tectonic settings. A qualitative analysis of written responses revealed limited changes in participants’ use of key terms before and after the video intervention. These findings suggest that while video-based instruction can reinforce core concepts, greater attention is needed to address cognitive load and support learning of more challenging topics. This study underscores the importance of integrating accessible, dynamic teaching tools and refining instructional design to better engage students with metamorphic processes, which are essential to understanding Earth’s dynamic systems.
The Investigation of Trends and Wet and Dry Rainfall Cycles in North Africa (In Morocco, Algeria, and Tunisia) (1970–2023)
Authors: Nouaceur Zeineddine , Murarescu Ovidiu , Muratoreanu George
Published: February 2025

IPCC climate forecast models, applicable to the Maghreb countries (Morocco, Algeria, and Tunisia), predict a decrease in atmospheric precipitation, greater variability, and an increase in aridity. In recent years, the entire region has been experiencing unprecedented climate upheavals. Climatic droughts have become increasingly severe and recurrent (drastically reducing water stocks). We are also witnessing a remarkable increase in temperatures and a greater frequency of heat waves. Faced with these new provisions, this territory (long considered an area of water stress) is now subject to very strong tensions, which have led to a greater demand for water and a decrease in supply. To understand the intensity of this “climate–water” crisis, we propose an analysis of this priority issue based on the evolution of precipitation over more than half a century of records. To determine precipitation trends and define rainfall cycles in these three countries, the graphical chronological method of information processing (MGCTI) of the “BERTIN Matrix” type is used. Annual precipitation totals from 29 stations were used for the MGCTI (chronological graphic method of information processing) for the period 1970–2023. These data come from the national meteorological networks of the National Meteorological Office (ONM) for Algeria, National Institute of Meteorology (NIM) for Tunisia, and National Directorate of Meteorology (DMN) for Morocco, and the from the websites of the National Climatic Data Center (NCDC) and “TuTiempo Network”. Monthly pluviometric totals from three stations, Dar El Beida (Algeria), Casablanca (Morocco), and Tunis (Tunisia), as well as the monthly NAO (North Atlantic Oscillation) and MOI (Mediterranean Oscillation Index) were used for the wavelet coherence method for the period 1970–2022. Data analysis shows that the entire region is subject to four extreme precipitation cycles (dry and wet). The last dry period was remarkably intense and led to a sharp increase in water stress throughout the region. An analysis of monthly precipitation from three stations (Casablanca, Dar El Beida, and Tunis) using the wavelet coherence method also highlighted a close relationship with the “NAO” and “MOI” circulation.
Eocene Gravity Flows in the Internal Prebetic (Betic Cordillera, SE Spain): A Vestige of an Ilerdian Lost Carbonate Platform in the South Iberian Margin
Authors: Tosquella Josep , Martín-Martín Manuel , Miclăuș Crina , Tent-Manclús José Enrique , Serrano Francisco , Martín-Pérez José Antonio
Published: February 2025

In the Betic-Rif Cordilleras, recent works have evidenced the existence of well-developed Eocene (Ypresian-Bartonian) carbonate platforms rich in Larger Benthic Foraminifera (LBF). Contrarily to other sectors of the western Tethys, like the Pyrenean domain in the North Iberian Margin, where these platforms started in the early Ypresian (Ilerdian), in the Betic-Rif chains, the recorded Eocene platforms started in the late Ypresian (Cuisian) after a widespread gap of sedimentation including the Ilerdian time span. In this work, the Aspe-Terreros Prebetic section (External Betic Zone) is studied. An Eocene succession with gravity flow deposits consisting of terrigenous and bioclastic turbidites, as well as olistostromes with olistoliths, was detected. In one of these turbidites, we dated (with the inherent limitations when dating bioclasts contained by gravity flow deposits) the middle Ilerdian, on the basis of LBF, representing a vestige of a missing Illerdian carbonate platform. The microfacies of these turbidites and olistoliths rich in LBF have been described and documented in detail. The gap in the sedimentary record and absence of Ilerdian platforms in the Betic-Rif Cordillera have been related to the so-called Eo-Alpine tectonics (Cretaceous to Paleogene) and sea-level variations contemporarily with the establishment of shallow marine realms in the margins of the western Tethys.
Diversity, Ethnobotany, and Horticultural Potential of Local Vegetables in Chai Chumphol Temple Community Market, Maha Sarakham Province, Thailand
Authors: Saensouk Piyaporn , Saensouk Surapon , Hein Khant Zaw , Appamaraka Sombat , Maknoi Charun , Sonthongphithak Phetlasy , Koompoot Kamonwan , Sonthongphithak Phiphat , Boonma Thawatphong , Jitpromma Tammanoon
Published: February 2025

Local markets are vital for economic activity and preserving cultural heritage, especially through vegetables central to community diets and traditions. Despite their importance, the ethnobotanical potential of these vegetables remains underexplored, particularly regarding sustainability and food security. This study examines the role of local vegetables in markets, highlighting their ecological, economic, and cultural significance. Conducted at the Chai Chumphol Temple community market in Kantarawichai District, Thailand, this research assessed the economic, cultural, and medicinal values of local vegetables. Various indices, including the economic value of vegetables (EVV), use value (UV), cultural importance (CI), informant agreement ratio (IAR), and fidelity level (%FL), were used to analyze the role of vegetables in the community. The findings underscore the importance of these vegetables in supporting local economies, preserving cultural traditions, and contributing to sustainable horticultural practices. These results highlight the potential of local vegetables to enhance horticultural biodiversity, promote food security, and support sustainable farming practices in the region.
Integrated Control of Powdery Mildew Using UV Light Exposure and OMRI-Certified Fungicide for Greenhouse Organic Lettuce Production
Authors: Ghalehgolabbehbahani Arash , Zinati Gladis , Hamido Said , Bhusal Nikita , Dhakal Madhav , Afshar Reza K. , Contina Jean B. , Caetani Romans , Smith Andrew , Panday Dinesh
Published: February 2025

Lettuce (Lactuca sativa L.) is a widely cultivated crop due to its short production cycle and high market demand. However, powdery mildew (Golovinomyces cichoracearum) poses a significant threat, reducing yields by up to 30% in various lettuce cultivars. This greenhouse study, conducted at the Rodale Institute in Pennsylvania, evaluated the impacts of pre-transplant UV light exposure and post-planting application of an OMRI-certified fungicide, potassium bicarbonate (MilStop), on powdery mildew infestation, yield, and nutritional quality of lettuce. The treatment included three factors: (a) UV-B (280 to 315 nm) exposure: treated vs. non-treated, (b) UV-C (100 to 280 nm) exposure: treated vs. non-treated, and (c) fungicide application: treated vs. non-treated, arranged in a factorial randomized complete block design with four replications. Lettuce seedlings (Salanova cultivar) were exposed to UV light before transplanting and later treated with MilStop. The results indicated that the combination of UV-B and MilStop significantly reduced powdery mildew infestation, while UV-C alone showed no significant effect. MilStop application enhanced lettuce yield, with treated plots showing a 44.8% increase in harvestable weight over control plots. While mineral and monosaccharide content were unaffected, UV-B exposure significantly increased total amino acid concentrations, including essential and non-essential amino acids. Pearson’s correlation analysis revealed a strong negative relationship between powdery mildew severity and harvestable weight, highlighting the importance of disease management. These findings highlight the potential of integrating UV light treatments and fungicide applications as effective, sustainable strategies for managing powdery mildew, improving lettuce yield, and maintaining nutritional quality in regenerative organic systems.
Culturally Sensitive Approaches in Psychosocial Interventions to Enhance Well-Being of Immigrant Adults Diagnosed with Breast Cancer: A Systematic Review
Authors: D’Souza Melba Sheila , Bacsu Juanita-Dawne , Sharma Arsh , Nairy Ashwin
Published: February 2025

Objective: The objective is to synthesize the literature on culturally sensitive approaches in psychosocial interventions to enhance the well-being of immigrant adults diagnosed with breast cancer. Methods: We conducted a systematic review following the guidelines for Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) and reporting literature searches, and a multi-database search strategy of qualitative research studies and reports published in academic journals and grey literature within a 20-year duration. Results: We extracted data from twenty-two studies that met the inclusion criteria. Content analysis revealed experiences of cultural considerations in the care and psychosocial well-being of immigrants such as the development of culturally responsive care models; barriers and gaps in culturally responsive care in rural communities; patient information, education, and culturally responsive care; cultural stigma, and self-perception of the access, use, and role of healthcare providers, the impact of cancer and linguistically appropriate care; and challenges with psychosocial well-being and culturally responsive care. Conclusions: Concerns relating to psychosocial well-being of immigrant adults diagnosed with breast cancer are consistently described in the literature. Interventions exist to address psychosocial well-being; however, none have been developed or tested in immigrant adults diagnosed with breast cancer. Addressing the psychosocial well-being of immigrant adults will require the integration of culturally appropriate considerations in care to attitudes impacting patient care and reported outcomes.
Function of lamp2 Gene Response to Vibrio vulnificus Infection and LPS Stimulation in the Half-Smooth Tongue Sole (Cynoglossus semilaevis)
Authors: Han Tian , Liu Yufeng , Li Mengchao , Zhang Yitong , He Zhongwei , Ren Yuqin , Cao Wei , Ren Jiangong , Wang Yufen , Wang Guixing , Gong Chunguang , Hou Jilun
Published: February 2025

Lysosome-associated membrane glycoproteins (LAMPs), including lysosomal membrane protein 1 (Lamp1) and lysosomal membrane protein 2 (Lamp2), are involved in phagocytosis, chaperone-mediated autophagy (CMA), and other pathways that interact with lysosomal activity. However, the role of Lamp2 in teleosts has not been clarified. In this study, we investigated the functions of lamp2 genes during Vibrio vulnificus infection. We achieved subcellular localization of the lamp2 gene at the cellular level and performed overexpression and RNA interference experiments followed by Lipopolysaccharides (LPS) stimulation to probe the expression changes of related genes. Ultrapathology analysis of the head-kidney revealed an increase in lysosomes and the formation of autophagosomal vesicles after V. vulnificus infection, suggesting that lysosomes bind to autophagosomes. The lamp2 gene, encoding 401 amino acids in Cynoglossus semilaevis, was constitutively expressed in all examined tissues of healthy half-smooth tongue sole, with the highest expression in blood. A challenge test was conducted to assess the response of half-smooth tongue sole (Cynoglossus semilaevis) to different concentrations of V. vulnificus. The results showed that the relative expression of lamp2 and its related genes—lc3, rab7, vamp8, atg14, stx17, snap29, ctsb, and ctsd—varied with time and concentration in the gill, spleen, head-kidney, blood, liver, and gut tissues. From the results of lamp2 gene overexpression and RNA interference experiments, it is hypothesized that lamp2 positively regulates lc3, rab7, vamp8, snap29, and stx17, and negatively regulates ctsd and ctsb. Our findings provide new primary data for the function of lamp2 gene in the half-smooth tongue sole., particularly its role in regulating the immune response against V. vulnificus.
Impact of Dietary Fiber on Inflammation in Humans
Authors: Kabisch Stefan , Hajir Jasmin , Sukhobaevskaia Varvara , Weickert Martin O. , Pfeiffer Andreas F. H.
Published: February 2025

Cohort studies consistently show that a high intake of cereal fiber and whole-grain products is associated with a decreased risk of type 2 diabetes (T2DM), cancer, and cardiovascular diseases. Similar findings are also reported for infectious and chronic inflammatory disorders. All these disorders are at least partially caused by inflammaging, a chronic state of inflammation associated with aging and Metabolic Syndrome. Surprisingly, insoluble (cereal) fiber intake consistently shows stronger protective associations with most long-term health outcomes than soluble fiber. Most humans consume soluble fiber mainly from sweet fruits, which usually come with high levels of sugar, counteracting the potentially beneficial effects of fiber. In both observational and interventional studies, high-fiber diets show a beneficial impact on inflammation, which can be attributed to a variety of nutrients apart from dietary fiber. These confounders need to be considered when evaluating the effects of fiber as part of complex dietary patterns. When assessing specific types of fiber, inulin and resistant starch clearly elicit anti-inflammatory short-term effects, while results for pectins, beta-glucans, or psyllium turn out to be less convincing. For insoluble fiber, promising but sparse data have been published so far. Hypotheses on putative mechanisms of anti-inflammatory fiber effects include a direct impact on immune cells (e.g., for pectin), fermentation to pleiotropic short-chain fatty acids (for fermentable fiber only), modulation of the gut microbiome towards higher levels of diversity, changes in bile acid metabolism, a differential release of gut hormones (such as the glucose-dependent insulinotropic peptide (GIP)), and an improvement of insulin resistance via the mTOR/S6K1 signaling cascade. Moreover, the contribution of phytate-mediated antioxidative and immune-modulatory means of action needs to be considered. In this review, we summarize the present knowledge on the impact of fiber-rich diets and dietary fiber on the human inflammatory system. However, given the huge heterogeneity of study designs, cohorts, interventions, and outcomes, definite conclusions on which fiber to recommend to whom cannot yet be drawn.
Defined Diets Link Iron and α-Linolenic Acid to Cyp1b1 Regulation of Neonatal Liver Development Through Srebp Forms and LncRNA H19
Authors: Jefcoate Colin R. , Larsen Michele C. , Song Yong-Seok , Maguire Meghan , Sheibani Nader
Published: February 2025

Cyp1b1 substantially affects hepatic vascular and stellate cells (HSC) with linkage to liver fibrosis. Despite minimal hepatocyte expression, Cyp1b1 deletion substantially impacts liver gene expression at birth and weaning. The appreciable Cyp1b1 expression in surrounding embryo mesenchyme, during early organogenesis, provides a likely source for Cyp1b1. Here defined breeder diets established major interconnected effects on neonatal liver of α-linolenic acid (ALA), vitamin A deficiency (VAD) and suboptimal iron fed mice. At birth Cyp1b1 deletion and VAD each activated perinatal HSC, while suppressing iron control by hepcidin. Cyp1b1 deletion also advanced the expression of diverse genes linked to iron regulation. Postnatal stimulations of Srebp-regulated genes in the fatty acid and cholesterol biosynthesis pathways were suppressed by Cyp1b1-deficiency. LncRNA H19 and the neutrophil alarmin S100a9 expression increased due to slower postnatal decline with Cyp1b1 deficiency. VAD reversed each of Cyp1b1 effect, probably due to enhanced HSC release of Apo-Rbp4. At birth, Cyp1b1 deletion enhanced H19 participation. Notably, a suppressor (Cnot3) decreased while an activity partner (Ezh2/H3K methylation) increased H19 expression. ALA elevated hepcidin mRNA and countered the inhibitory effects of Cyp1b1 deletion on hepcidin expression. Oxylipin metabolites of ALA from highly expressed hepatic Cyps are potential mediators. Cyp expression patterns demonstrated female dimorphism for neonatal liver. Mothers followed one of three fetal growth support programs probably linked to maturity at conception.
Evaluation of Adaptive Responses of Juglans neotropica Diels Progenies Based on Dasometric Traits
Authors: Ramos-Veintimilla Raúl Armando , Romero-Cañizares Fernando , González-Narváez Mariela , Vera-Velez Roy , García-Mora Mario
Published: February 2025

(1) Background: Juglans neotropica Diels, native to the Andes, is highly valued for its quality wood, medicinal uses, edible fruits, and natural dyes. However, its population has been greatly reduced due to overexploitation, becoming threatened and resulting in genetic stochasticity. Therefore, a prompt ex situ and in situ conservation effort is needed for its conservation and restoration. (2) Methods: A total of 439 trees of J. neotropica Diels were sampled from selected individuals across the northern and central regions of Ecuador. These trees were planted in a randomized complete block design to assess their growth and genetic variability. (3) Results: Annual average tree growth varied significantly among progenies. Based on their dasometric traits, two distinct groups were identified as superior and surveillance. Five trees demonstrated promising traits (TJ182, ChL2314, ChL142, TJ1310, and BSM14), suggesting potential for inclusion in forest genetic improvement programs. (4) Conclusions: Fifteen percent of individuals from the five studied provenances exhibited desirable dasometric characteristics and high-quality wood. In addition, several individuals within the progenies exhibit resistance to biotic agents, indicating a genetic potential for disease.
Existence and Mass Gap in Quantum Yang–Mills Theory
Authors: Nye Logan
Published: February 2025

This paper presents a novel approach to solving the Yang–Mills existence and mass gap problem using quantum information theory. We develop a rigorous mathematical framework that reformulates the Yang–Mills theory in terms of quantum circuits and entanglement structures. Our method provides a concrete realization of the Yang–Mills theory that is manifestly gauge-invariant and satisfies the Wightman axioms. We demonstrate the existence of a mass gap by analyzing the entanglement spectrum of the vacuum state, establishing a direct connection between the mass gap and the minimum non-zero eigenvalue of the entanglement Hamiltonian. Our approach also offers new insights into non-perturbative phenomena such as confinement and asymptotic freedom. We introduce new mathematical tools, including entanglement renormalization for gauge theories and quantum circuit complexity measures for quantum fields. The implications of our work extend beyond the Yang–Mills theory, suggesting new approaches to quantum gravity, strongly coupled systems, and cosmological problems. This quantum information perspective on gauge theories opens up exciting new directions for research at the intersection of quantum field theory, quantum gravity, and quantum computation.
Validation of a Novel Suprathreshold Strategy for Screening Visual Function
Authors: Arias Juan D. , Chopra Reena , Durbin Mary K. , Knight Kristen N. , Ho Derek Y. , Miranda Marco A. , Hou Huiyuan , Forte Christina , Fanelli James L.
Published: February 2025

Background/Objectives: Glaucoma, a leading cause of blindness worldwide, is often associated with high intraocular pressure (IOP), which eventually leads to loss of retinal ganglion cells and the retinal nerve fiber layer. Visual field (VF) testing is a principal method of diagnosing and monitoring this disease. Suprathreshold VF test programs are quicker than threshold strategies and are often used as a screening tool. This study evaluates the TEMPO/IMOvifa (Topcon Healthcare/CREWT Medical Systems, Tokyo, Japan), a bilateral standard automated perimeter with a suprathreshold screening program by assessing the sensitivity in a glaucoma cohort and the specificity in a healthy cohort. Methods: All subjects were tested at a single site and underwent a comprehensive ocular examination to categorize them into either a healthy or glaucoma group. As part of the testing procedure, two TEMPO suprathreshold VFs were conducted in sequence and accompanied by a threshold VF test. Results: A total of 193 eyes (randomized study eye) (193 subjects) were evaluated in the final analysis (101 healthy and 92 glaucoma), and average suprathreshold test time (SD) per eye was 39.4 (±4.86) seconds. Specificity was at 91% in the healthy group and sensitivity was at 49% in the glaucoma group. Sensitivity was at 100% when applied to glaucoma cases with an MD of less than −3 dB. Conclusions: The TEMPO screening program demonstrated strong specificity in detecting true healthy cases. It also demonstrated a strong sensitivity when screening mild to moderate glaucoma. Early glaucoma and glaucoma suspects would benefit from complementary modalities such as optical coherence tomography and threshold perimetry to effectively diagnose. Utilizing this screening program in optometric and ophthalmic settings could yield benefits for both the practitioner and the patient.
Effects of Vernix Caseosa on Cesarean Skin Incision: A Double-Blind Study
Authors: Sapmaz Mehmet Alican , Polat Murat , Erbey Sait , Eroglu Omer Osman , Kahyaoglu Inci
Published: February 2025

Background/Objectives: Vernix caseosa is a natural protective barrier with moisturizing, wound healing, and antioxidant properties. This study aimed to evaluate the effects of vernix caseosa on skin pigmentation and wound healing in cesarean section incisions. Methods: This randomized, double-blind, placebo-controlled trial was conducted at Ankara Etlik City Hospital, Gynecology and Obstetrics Clinic. A total of 210 first-time cesarean section patients were included. Vernix caseosa, collected from newborns, was applied to the incision site in half of the participants, while the other half received a placebo. Scar assessment was performed using the Manchester Scar Scale and the Patient and Observer Scar Assessment Scale (POSAS). Results: The vernix group showed significantly lower Manchester Scar Scale scores (9.00 ± 2.07 vs. 12.60 ± 2.72, p < 0.001) and POSAS total scores (53.57 ± 20.48 vs. 89.60 ± 26.54, p < 0.001) compared to the control group. These results suggest that vernix caseosa accelerates wound healing and improves cosmetic outcomes. Conclusions: Vernix caseosa emerges as a natural, effective, and low-cost method to promote wound healing in cesarean section incisions. Its medical and cosmetic benefits support its use in postoperative care. Future studies could further validate these findings in broader populations and explore additional clinical applications.
Polymerized Molecular Allergoid Alt a1: Effective SCIT in Pediatric Asthma Patients
Authors: Brindisi Giulia , Gori Alessandra , Anania Caterina , De Castro Giovanna , Spalice Alberto , Loffredo Lorenzo , Salvatori Alessandra , Zicari Anna Maria
Published: February 2025

Background: Allergy to Alternaria alternata (Alt a), although often underdiagnosed, is a significant global health issue. In the allergen immunotherapy (AIT) field, novel therapeutic strategies are emerging, particularly with the advent of polymerized allergoids. This study aims to evaluate the efficacy of subcutaneous immunotherapy (SCIT) based on these innovative molecules in children with respiratory allergies, assessing clinical and functional parameters. Methods: We enrolled 42 patients aged between 6 and 16 years, all of whom had allergic rhinitis (AR) and concomitant asthma and all of whom were monosensitized to Alt a. Between December 2020 and December 2021, 17 patients initiated SCIT with Modigoid® for Alt a1, while 25 patients continued with standard therapy. At the initial visit (T0), all the patients underwent nasal and bronchial evaluation, including exhaled nitric oxide (eFeNO) measurement and spirometry. The Asthma Control Test (ACT) was used to evaluate the control of asthma symptoms. Patients were followed up every 6 months, with a comprehensive re-evaluation at 24 months (T1) replicating the initial assessments. Results: After 24 months of SCIT with the new polymerized molecular allergoid Alt a1 (Modigoid®), children showed a statistically significant reduction in eFeNO levels, improved FEV1 values, and enhanced ACT scores. Conclusions: SCIT with the new molecular allergoid Alt a1 significantly improves functional parameters (FEV1 and eFeNO) and subjective asthma symptoms (ACT scores) in children with AR and objective asthma signs. This treatment represents an effective preventive strategy that can be used to halt the progression of the classic atopic march from AR to asthma and potentially reverse the atopic march.
Perspectives in the Diagnosis, Clinical Impact, and Management of the Vulnerable Plaque
Authors: Alperi Alberto , Antuna Paula , Almendárez Marcel , Álvarez Rut , del Valle Raquel , Pascual Isaac , Hernández-Vaquero Daniel , Avanzas Pablo
Published: February 2025

Coronary artery disease is a highly prevalent disease that constitutes the leading cause of mortality worldwide. Acute coronary syndromes are the most devastating form of presentation of coronary disease, involving the acute formation of a thrombus within the coronary vessel lumen, further leading to flow limitation and diminished myocardial perfusion. Vulnerable plaques, which are characterized by thin-cap fibroatheroma, a large lipid pool, and macrophage infiltration and spotty calcification of the cap, pose a higher risk of coronary events despite not being flow-limiting. Iterations in intravascular imaging and coronary computed tomography have largely increased the ability to detect and define vulnerable plaques, and its clinical impact in early- and mid-term outcomes has been confirmed in several studies. In this review, we aimed to revise the current concept of vulnerable coronary plaque and its repercussion, to summarize the main pharmacological approaches for its management, and to provide an updated overview of the available evidence on preventive percutaneous interventional strategies in this clinical setting.
A Multicomponent Training Program Improves Physical Function and Quality of Life for a Mesenchymal Chondrosarcoma Survivor Subjected to Internal Hemipelvectomy: A Case Study
Authors: Guerrero Romero Lucía , Cepero González Mar , Rojas-Ruiz Francisco J.
Published: February 2025

Background/Objectives: Overcoming an oncological process has a significant impact on lower-extremity sarcoma survivors’ quality of life, due to the deterioration in their physical and functional state. This study evaluated the effects of a six-month multicomponent physical training program on the physical function and quality of life of a mesenchymal chondrosarcoma survivor. Methods: The mesenchymal chondrosarcoma survivor was subjected to an internal hemipelvectomy including right hemipelvis plus femoral joint and resection of the right proximal femur followed by chemotherapy and radiotherapy according to the oncology protocol. The program was performed twice weekly and included resistance, cardiorespiratory, trunk control, and stability exercises. Results: Functional assessments revealed improvements in hand grip strength, walking speed, balance, and coordination. The Timed Up and Go Test showed a 50% reduction in completion time, reflecting better mobility and strength. Additionally, gait speed increased significantly, and balance trials indicated enhanced coordination. Quality of life evaluations indicated progress in physical health, psychological well-being, and environmental engagement. Conclusions: Taken together, this research emphasizes the importance of tailored exercise interventions for sarcoma survivors, particularly those with significant physical impairments. Such programs are vital complements to conventional rehabilitation strategies, fostering physical activity adapted to individual needs. By addressing the multifaceted challenges of survivorship, these interventions enhance functional capacity, reduce disability, and improve overall well-being. Therefore, this case study highlights the program’s effectiveness in managing post-treatment sequelae, opening a pathway to improved physical autonomy and quality of life.
Age-Related Variations in the Clinical Presentation and Treatment Outcomes of New-Onset GPA: A Longitudinal Study
Authors: Potentas-Policewicz Malgorzata , Gawryluk Dariusz , Wiatr Elzbieta , Fijolek Justyna
Published: February 2025

Background/Objectives: This study compares the clinical features and treatment outcomes of granulomatosis with polyangiitis (GPA) based on age at onset. Methods: A retrospective longitudinal cohort of patients with GPA diagnosed between January 1978 and December 2015 was analyzed, stratified by age at diagnosis: ≤30 years (young group), 31–59 years (middle-aged group), and ≥60 years (older group). The comparative analysis included demographic data, organ involvement, laboratory results, anti-neutrophil cytoplasmic antibody (ANCA) status, comorbidities, treatments, and outcomes. Results: The analysis included 264 patients newly diagnosed with GPA. Older patients exhibited significantly higher rates of peripheral neuropathy and liver involvement. They had more severe lung diseases and required lung biopsies more frequently. Patients in the middle-aged group exhibited the highest likelihood of severe anemia. Peripheral neuropathy was more common in this group than in younger patients, and their lung disease was less severe than in older patients but more severe than in younger patients. Young patients exhibited mild disease with the least severe lung involvement, mild anemia, and highest albumin levels. Baseline comorbidities and post-treatment adverse events increased significantly with age at diagnosis. Treatment strategies and efficacy were similar across groups, although older patients tended to receive lower initial doses of cyclophosphamide and corticosteroids. Conclusions: Age at diagnosis influenced GPA clinical characteristics. While the treatment did not vary significantly by age at onset, tailoring therapy to a patient’s age is crucial to optimize outcomes and minimize complications.
Prognostic Value of Chest CT Volumetric Analysis in Patients with Malignant Pleural Mesothelioma
Authors: Baratella Elisa , Ercolani Eleonora , Segalotti Antonio , Troian Marina , Lovadina Stefano , Giudici Fabiola , Minelli Pierluca , Ruaro Barbara , Salton Francesco , Cova Maria Assunta
Published: February 2025

Background/Objectives: Malignant pleural mesothelioma (MPM) is a rare, aggressive cancer linked to asbestos exposure and with poor overall survival. In recent years, CT volumetric analysis has gained increasing interest as a more accurate method for assessing tumor burden. This study aims to evaluate the prognostic value of chest CT volumetric analysis in MPM, comparing tumor volume with tumor thickness measurements and survival outcomes. Methods: This is a retrospective, observational analysis of all patients undergoing diagnostic thoracoscopy between 2014 and 2021 at the University Hospital of Cattinara (Trieste, Italy). Inclusion criteria were as follows: age ≥ 18 years, histological diagnosis of MPM, and the availability of at least one contrast-enhanced chest CT scan at the time of diagnosis. For each patient, the tumor thickness was measured on the axial plane at three levels (upper, middle, and lower hemithorax). Tumor and effusion volumes were calculated with the RayStation® software version 11.7.174 (HealthMyne®, Madison, WI, USA). Results: A total of 81 patients were eligible for analysis. Maximum and mean tumor thickness were strongly associated with survival, with higher thicknesses correlating with an increased risk of death (adjusted hazard ratio per doubling (aHR) of 1.97 (95%CI: 1.40–2.77) and of 2.23 (95%CI: 1.56–3.20), p < 0.001)), respectively, while the effect of the tumor volume on survival was nevertheless significant but less impactful (aHR = 1.26 (1.10–1.45, p < 0.001)). The presence and volume of effusion did not correlate with survival (p = 0.48 and p = 0.64, respectively). Conclusions: This study supports the role of quantitative parameters for staging MPM, particularly given the frequent discrepancies between clinical and pathological staging when relying solely on qualitative measures.