bluuebunny/crossref_metadata_2025 huggingface dataset:
Epigenetic regulation of macrophage activation in chronic obstructive pulmonary disease
Authors: Zhang Feng , Cui Yachao , Zhang Tiejun , Yin Wenguang
Published: August 2024

Macrophages in the innate immune system play a vital role in various lung diseases such as asthma, chronic obstructive pulmonary disease (COPD), acute lung injury and pulmonary fibrosis. Macrophages involved in the process of immunity need to go through a process of activation, including changes in gene expression and cell metabolism. Epigenetic modifications are key factors of macrophage activation including DNA methylation, histone modification and non-coding RNA regulation. Understanding the role and mechanisms of epigenetic regulation of macrophage activation can provide insights into the function of macrophages in lung diseases and help identification of potential therapeutic targets. This review summarizes the latest progress in the epigenetic changes and regulation of macrophages in their development process and in normal physiological states, and the epigenetic regulation of macrophages in COPD as well as the influence of macrophage activation on COPD development.
Fourier-transform near-infrared spectroscopy first application to age determination in European fish species: the case of the Atlantic horse mackerel from the central Mediterranean Sea
Authors: Basilone Gualtiero , Lo Cicero Gabriella , Fortuna Miryam , Luviner Anita , Ferreri Rosalia , Aronica Salvatore , Genovese Simona , Giacalone Giovanni , Fontana Ignazio , Bonanno Angelo
Published: August 2024

Fourier-transform near-infrared spectroscopy (FT-NIRS) has recently been used to reduce the processing time for estimates of annual age in several fish species. The present study represents the first application of this technique to marine organisms from the European waters. Atlantic horse mackerel (Trachurus trachurus) from the central Mediterranean Sea was selected for its ecological role, its socioeconomic value, and because its age is regularly estimated by otolith reading under a stereomicroscope for stock assessment purposes. Absorption spectra of the whole otoliths were acquired by FT-NIRS across a multiyear dataset, obtained during acoustic surveys carried out in different regions of the central Mediterranean Sea. The acquired spectra were processed to optimize calibration models to predict age. The best linear models obtained by the optimizing procedure predicted age successfully with a coefficient of determination of 0.95–0.96, mean squared error of 0.5 years, and bias <0.03 years. Although regional calibration models were also obtained separately, the models with all regions combined appeared much more robust and encompassing the whole age range, suggesting its usefulness for the entire spatial distribution range considered in the preset study. The use of FT-NIRS allowed to successfully predict age for horse mackerel between the ages 1 and 14, with similar or better precision (and bias) of the traditional methods, also increasing the standardization by repeatability of age assignments. The preliminary results obtained by this study encourage further effort to fine-tune the calibration model for new species, in order to apply a precise, rapid, cost-effective method for stock assessment purposes of Mediterranean fish resources.
Advances in the study of AIE polymers
Authors: Pei Yiran , Liu Leixin , Cao Xinfeng , Zhou Jian , Liu Cuiyun
Published: August 2024

Aggregation-induced emission (AIE) can exhibit different properties in different situations, such as non-emission and highly fluorescent in the dissolved state of the molecule and in the aggregate or solid state, respectively. This property of AIE is distinguished from aggregation-induced quenching (ACQ) or even the opposite. Combining the AIE phenomenon with different polymers yields different polymers with corresponding AIE properties. In this paper, the mechanism, synthesis, branching and application of AIE in the fields of optoelectronic functional materials, sensors, biology, and environment are reviewed. It is hoped that this review will stimulate more research on molecular aggregates and promote further cross-fertilisation and greater development in the disciplines of materials, chemistry and biomedicine.
Effects of season and water quality on community structure of planktonic eukaryotes in the Chaohu Lake Basin
Authors: Zhang Yan , Han Maozhen , Wu Li , Ding Guoao , Liu Kai , He Kui , Zhao Jingqiu , Liao Yiwen , Gao Yun , Zhang Cui
Published: August 2024

Introduction

Analyzing the correlation between planktonic eukaryotic communities (PECs) and aquatic physicochemical parameters (APPs) provides important references for predicting the impact of climate change and human activities on aquatic ecosystems.

Methods

To assess the influence of seasons and APPs on PEC structures in lakes and rivers, we utilized high-throughput sequencing of the 18S rRNA gene to analyze PEC structures in a lake and seven rivers in the Chaohu Lake Basin and analyzed their correlations with APPs.

Results

Our results revealed that PEC structure was significantly affected by season, with the highest α-diversity observed in summer. Furthermore, we identified several APPs, including water temperature, conductivity, dissolved oxygen, pH, phosphate, total phosphorus, trophic level index (TLI), nitrate, ammonia nitrogen, and total nitrogen, that significantly influenced PEC structures. Specifically, we found that Stephanodiscus hantzschii, Simocephalus serrulatus, Cryptomonas sp. CCAC_0109, Pedospumella encystans, Actinochloris sphaerica, Chlamydomonas angulosa, Gonyostomum semen, Skeletonema potamos, Chlamydomonas klinobasis, Pedospumella sp., and Neochlorosarcina negevensis were significantly correlated to TLI, while Limnoithona tetraspina, Theileria sp., and Pseudophyllomitus vesiculosus were significantly correlated to the water quality index (WQI). However, our random forest regression analysis using the top 100 species was unable to accurately predict the WQI and TLI.

Discussion

These results provide valuable data for evaluating the impact of APPs on PEC and for protecting water resource in the Chaohu Lake Basin.
Microbes as carbendazim degraders: opportunity and challenge
Authors: Zhou Yi , Wang Tianyue , Wang Liping , Wang Pengfei , Chen Feiyu , Bhatt Pankaj , Chen Shaohua , Cui Xiuming , Yang Ye , Zhang Wenping
Published: August 2024

Carbendazim (methyl benzimidazol-2-ylcarbamate, CBZ) is a systemic benzimidazole carbamate fungicide and can be used to control a wide range of fungal diseases caused by Ascomycetes, Basidiomycetes and Deuteromycetes. It is widely used in horticulture, forestry, agriculture, preservation and gardening due to its broad spectrum and leads to its accumulation in soil and water environmental systems, which may eventually pose a potential threat to non-target organisms through the ecological chain. Therefore, the removal of carbendazim residues from the environment is an urgent problem. Currently, a number of physical and chemical treatments are effective in degrading carbendazim. As a green and efficient strategy, microbial technology has the potential to degrade carbendazim into non-toxic and environmentally acceptable metabolites, which in turn can dissipate carbendazim from the contaminated environment. To date, a number of carbendazim-degrading microbes have been isolated and reported, including, but not limited to, Bacillus, Pseudomonas, Rhodococcus, Sphingomonas, and Aeromonas. Notably, the common degradation property shared by all strains was their ability to hydrolyze carbendazim to 2-aminobenzimidazole (2-AB). The complete mineralization of the degradation products is mainly dependent on the cleavage of the imidazole and benzene rings. Additionally, the currently reported genes for carbendazim degradation are MheI and CbmA, which are responsible for breaking the ester and amide bonds, respectively. This paper reviews the toxicity, microbial degradation of carbendazim, and bioremediation techniques for carbendazim-contaminated environments. This not only summarizes and enriches the theoretical basis of microbial degradation of carbendazim, but also provides practical guidance for bioremediation of carbendazim-contaminated residues in the environment.
Transcriptomic responses to shifts in light and nitrogen in two congeneric diatom species
Authors: Ma Xiao , Qin Zhen , Johnson Kevin B. , Sweat L. Holly , Dai Sheng , Li Gang , Li Chaolun
Published: August 2024

Light and nitrogen availability are basic requirements for photosynthesis. Changing in light intensity and nitrogen concentration may require adaptive physiological and life process changes in phytoplankton cells. Our previous study demonstrated that two Thalassiosira species exhibited, respectively, distinctive physiological responses to light and nitrogen stresses. Transcriptomic analyses were employed to investigate the mechanisms behind the different physiological responses observed in two diatom species of the genus Thalassiosira. The results indicate that the congeneric species are different in their cellular responses to the same shifting light and nitrogen conditions. When conditions changed to high light with low nitrate (HLLN), the large-celled T. punctigera was photodamaged. Thus, the photosynthesis pathway and carbon fixation related genes were significantly down-regulated. In contrast, the small-celled T. pseudonana sacrificed cellular processes, especially amino acid metabolisms, to overcome the photodamage. When changing to high light with high nitrate (HLHN) conditions, the additional nitrogen appeared to compensate for the photodamage in the large-celled T. punctigera, with the tricarboxylic acid cycle (TCA cycle) and carbon fixation significantly boosted. Consequently, the growth rate of T. punctigera increased, which suggest that the larger-celled species is adapted for forming post-storm algal blooms. The impact of high light stress on the small-celled T. pseudonana was not mitigated by elevated nitrate levels, and photodamage persisted.
Biochemical and structural elucidation of the L-carnitine degradation pathway of the human pathogen Acinetobacter baumannii
Authors: Piskol Fabian , Lukat Peer , Kaufhold Laurin , Heger Alexander , Blankenfeldt Wulf , Jahn Dieter , Moser Jürgen
Published: August 2024

Acinetobacter baumannii is an opportunistic human pathogen which can use host-derived L-carnitine as sole carbon and energy source. Recently, an L-carnitine transporter (Aci1347) and a specific monooxygense (CntA/CntB) for the intracellular cleavage of L-carnitine have been characterized. Subsequent conversion of the resulting malic semialdehyde into the central metabolite L-malate was hypothesized. Alternatively, L-carnitine degradation via D-malate with subsequent oxidation into pyruvate was proposed. Here we describe the in vitro and in vivo reconstitution of the entire pathway, starting from the as yet uncharacterized gene products of the carnitine degradation gene operon. Using recombinantly purified enzymes, enantiomer-specific formation of D-malate by the NAD(P)+-dependent malic semialdehyde dehydrogenase (MSA-DH) is demonstrated. The solved X-ray crystal structure of tetrameric MSA-DH reveals the key catalytic residues Cys290 and Glu256, accessible through opposing substrate and cofactor funnels. Specific substrate binding is enabled by Arg166, Arg284 and Ser447 while dual cofactor specificity for NAD+ and NADP+ is mediated by Asn184. The subsequent conversion of the unusual D-malate reaction product by an uncharacterized NAD+-dependent malate dehydrogenase (MDH) is shown. Tetrameric MDH is a β-decarboxylating dehydrogenase that synthesizes pyruvate. MDH experiments with alternative substrates showed a high degree of substrate specificity. Finally, the entire A. baumannni pathway was heterologously reconstituted, allowing E. coli to grow on L-carnitine as a carbon and energy source. Overall, the metabolic conversion of L-carnitine via malic semialdehyde and D-malate into pyruvate, CO2 and trimethylamine was demonstrated. Trimethylamine is also an important gut microbiota-dependent metabolite that is associated with an increased risk of cardiovascular disease. The pathway reconstitution experiments allowed us to assess the TMA forming capacity of gut microbes which is related to human cardiovascular health.
Contribution of advanced neuroimaging in diagnosis of cerebral syphilitic gumma: a case report
Authors: Shen Xinyi , Zhu Zhengyang , Li Xin , Zhang Wen , Zhang Xin , Zhang Bing
Published: August 2024

Background

Cerebral syphilitic gumma is a rare intracranial infectious disorder. Without a clear history of syphilis and comprehensive serological examinations, it’s challenging to diagnose it accurately prior to surgery through routine magnetic resonance imaging (MRI). Advanced neuroimaging techniques have been widely used in diagnosing brain tumors, yet there’s limited report on their application in cerebral syphilitic gumma. This report presents a case of an elderly male patient with cerebral syphilitic gumma and analyzes its characteristics of advanced neuroimaging.

Case presentation

A 68-year-old male patient was admitted to our institution presenting with bilateral hearing loss complicated with continuing headaches without obvious cause. Laboratory tests indicated positive treponema pallidum. Conventional MRI showed nodules closely related to the adjacent meninges in bilateral temporal lobes. The patient underwent surgical resection of the nodule in the right temporal lobe due to the mass effect and the final pathological diagnosis revealed cerebral syphilitic gumma.

Conclusions

With the return of syphilis in recent years, accurate diagnosis of cerebral syphilitic gumma is a matter of great urgency. Advanced neuro-MRI can serve as a significant complement to conventional MRI examination.
Parent-child skin carotenoid level and vegetable intake relationships in users of children’s cafeterias in Japan
Authors: Kurotani Kayo , Ohkawara Kazunori , Takimoto Hidemi
Published: August 2024

Introduction

Studies on the relationship between parental and child dietary intakes are limited in Asian populations. Here, we examined parent-child relationships in skin carotenoid levels and vegetable intake in a Japanese community.

Methods

The study participants were 58 children aged 6–15 years and 39 of their guardians (parents) using children’s cafeterias. Skin carotenoid levels were measured using the Veggie Meter®, and the number of vegetable dishes (equivalent to a serving of 70 g) was evaluated using a self-administered questionnaire.

Results

The mean (standard deviation; SD) skin carotenoid levels were 366.8 (74.0) in children and 315.0 (101.4) in parents. The partial correlation coefficient between parents’ and children’s skin carotenoid levels, adjusting for cafeteria, sex, parental dietary supplement use, and household financial status, was 0.38 (P = 0.02); after adjustment for smoking status and BMI, the positive correlation was attenuated (r = 0.25, P = 0.14). A positive correlation was observed between parents’ and children’s vegetable dish intake (r = 0.30, P = 0.02).

Conclusion

This cross-sectional study identified a positive correlation between parent-child intake of vegetable dishes, accounting for potential confounders. However, the positive correlation observed between parent-child skin carotenoid levels may have been attenuated by internal factors such as smoking and obesity.
Association between dietary copper, iron, zinc, selenium intake and osteopenia or osteoporosis in elderly hypertensive patients: a retrospective cohort study
Authors: Chen Mingji , Jia Long , Gao Rufeng
Published: August 2024

Aim

The study aimed to investigate the link between dietary copper, iron, zinc, selenium intake with osteopenia and osteoporosis in elderly hypertensive patients.

Methods

The data of hypertensive patients were extracted from the National Health and Nutrition Examination Survey 2005–2010, 2013–2014, and 2017–2018. Data of dietary iron, zinc, copper and selenium intakes were obtained according to 24-h diet recall interviews. Osteopenia and osteoporosis were determined based on the bone mineral density. Weighted liner regression and weighted logistic regression were employed to assess the association between iron, zinc, copper, and selenium intakes with osteopenia and osteoporosis. All results were presented as β, odds ratios (ORs), and 95% confidence intervals (CIs).

Results

In total, 5,286 elderly hypertensive patients were included. Among them, 2,961 (56.02%) patients have osteopenia, and 566 (10.71%) have osteoporosis. After adjusting all covariates, dietary copper intake ≥the recommended daily allowance was positively correlated with bone mineral density on total femur (β = 0.086, 95% CI: 0.021–0.152) and femoral neck (β = 0.097, 95% CI: 0.016–0.178). Dietary zinc intake ≥the recommended daily allowance was also positively correlated with bone mineral density on total femur (β = 0.092, 95% CI: 0.030–0.153) and femoral neck (β = 0.122, 95% CI: 0.050–0.193). Dietary copper (O = 0.581, 95% CI: 0.394–0.858) and zinc (OR = 0.595, 95% CI: 0.429–0.827) intake ≥the recommended daily allowance levels were related to increased odds of osteoporosis in elderly with hypertension.

Conclusion

Higher dietary copper and zinc intake was associated with lower odds of osteoporosis in the elderly hypertensive patients. Higher dietary intake included copper and zinc may be beneficial for the bone health in the elderly hypertensive patients.
Adaptability and nutritional analysis of a newly isolated Chlorella sp. NeZha in brackish and marine environments with potential bioeconomic impacts
Authors: Yuan Shuai , Du Ming , Li Xianhui , Xu Ke , Zhang Kaining , Liu Xiaoya , Wang Jiangxin
Published: August 2024

Introduction

The microalga Chlorella sp. NeZha, recently isolated from a balcony environment, shows significant adaptability across various salinity conditions, including seawater (SeaW), freshwater (FreshW), and high salinity levels (45‰). This study investigates its potential for sustainable aquaculture and biotechnological applications.

Methods

Morphological and genetic identification were conducted using optical microscopy and DNA sequencing. The microalga was cultivated in a 400 L outdoor photobioreactor, and its biochemical composition, including chlorophyll a, carbohydrate, protein, and lipid content, was analyzed. Its compatibility with zooplankton and growth in aquaculture wastewater were also evaluated.

Results

Chlorella sp. NeZha produced chlorophyll a at concentrations exceeding seaweed and Spirulina by 10- and 5-fold, respectively, with a dry weight chlorophyll a content of 34.25 mg/g and 25 pg./cell. The microalga also contained carbohydrate (~33%), protein (~20%), and lipids (~14%). It was compatible with zooplankton species, such as rotifers and brine shrimp, and showed promising growth in aquaculture wastewater.

Discussion

The findings suggest that Chlorella sp. NeZha is a viable candidate for sustainable aquaculture and biotechnological applications, offering high nutritional value and environmental resilience. Its adaptability to diverse salinity conditions and ability to thrive in wastewater highlight its potential for bioremediation and use as feedstock for zooplankton. Further research is recommended to optimize its cultivation and explore broader applications.
The signature genes of cuproptosis associates with tumor immune microenvironment and predicts prognosis in kidney renal clear cell carcinoma
Authors: Liu Shuhan , Lv Shijie , Li Xi , Lu Weiguo , Chen Shengjie
Published: August 2024

Background

Cuproptosis is a new form of cell death, which has great potential to be developed in tumors treatment. Our study aimed to explore the predictive value of cuproptosis-related genes (CRGs) in various cancers, with a focus on kidney renal clear cell carcinoma (KIRC).

Method

A total of 9502 pan-cancer patients from TCGA cohort were enrolled. The relationships between CRGs and overall survival (OS) or disease-free survival (DFS) were analyzed. Gene Set Variation Analysis (GSVA) enrichment analysis was performed to explore the expression differences of CRGs. Multivariate Cox regression analysis was used to evaluate the association between GSVA scores and patient survival. KEGG and GO analyses were employed to identify the biological functions and pathways. The expression and prognostic characteristics of FDX1 were examined to evaluate the correlation between FDX1 and KIRC. Cell experiments were conducted to verify whether FDX1 was involved in cuproptosis of Caki-1 cells induced by Elesclomol.

Results

Positive cuproptosis signature genes(pos.cu.sig) exhibited the correlation with prognosis in KIRC, and all of these genes showed differential expression between KIRC and normal tissues. The GSVA score of pos.cu.sig was associated with excellent survival (HR=0.61, P<0.05), which can also serve as an independent prognostic factor for KIRC. There was a close correlation between pos.cu.sig and the tumor immune microenvironment in KIRC by KEGG and GO analysis. FDX1 expression was correlated with KIRC grade and positively associated with prognosis in KIRC patients. Compared with the control group, cell proliferation and migration were significantly inhibited, FDX1 expression was up-regulated, and Fe-S cluster protein content was decreased of Caki-1 cells after Elesclomol treatment.

Conclusions

This study provides compelling evidence that cuproptosis is closely linked to the prognosis of KIRC. FDX1 holds promise as a viable biomarker and therapeutic target for assessing the effectiveness of tumor immunotherapy in KIRC.
Immune-related intestinal pseudo-obstruction caused by immune checkpoint inhibitors: case report
Authors: Qian Yimeng , Zhi Zheng , Ai Jing , Kang Lin , Qiu Gang , Huang Xin , Zhao Jing
Published: August 2024

Intestinal obstruction, a rare manifestation of immunotherapy-related gastrointestinal adverse events, can be severe and even life-threatening with intestinal perforation. We present a 64-year-old man with HCC and currently under the therapy with Pembrolizumab, who was admitted in our hospital with abdominal distension. Radiologic findings were consistent with small bowel ileus. After conservative treatment, the patient underwent colonoscopy where no cause of ileus was discovered. The patient received high-dose prednisone due to the side effects of immune checkpoint inhibitor therapy. This resulted in a gradual improvement of symptoms.
Fer governs mTORC1 regulating pathways and sustains viability of pancreatic ductal adenocarcinoma cells
Authors: Schrier Ilan , Slotki-Itzchakov Orel , Elkis Yoav , Most-Menachem Nofar , Adato Orit , Fitoussi-Allouche Debora , Shpungin Sally , Unger Ron , Nir Uri
Published: August 2024

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers with a high percentage of morbidity. The deciphering and identification of novel targets and tools for intervening with its adverse progression are therefore of immense importance. To address this goal we adopted a specific inhibitor of the intracellular tyrosine kinase Fer, whose expression level is upregulated in PDAC tumors, and is associated with poor prognosis of patients. Subjecting PDAC cells to the E260-Fer inhibitor, unraveled its simultaneous effects on the mitochondria, and on a non-mitochondrial ERK1/2 regulatory cascade. E260 caused severe mitochondrial deformation, resulting in cellular- aspartate and ATP depletion, and followed by the activation of the metabolic sensor AMPK. This led to the phosphorylation and deactivation of the bona fide AMPK substrate, RAPTOR, which serves as a positive regulator of the mTORC1 metabolic hub. Accordingly, this resulted in the inhibition of the mTORC1 activity. In parallel, E260 downregulated the activation state of the ERK1/2 kinases, and their ability to neutralize the mTORC1 suppressor TSC2, thereby accentuating the inhibition of mTORC1. Importantly, both activation of AMPK and downregulation of ERK1/2 and mTORC1 were also achieved upon the knockdown of Fer, corroborating the regulatory role of Fer in these processes. Concomitantly, in PDAC tumors and not in healthy pancreatic tissues, the expression levels of Fer demonstrate moderate but statistically significant positive correlation with the expression levels of mTOR and its downstream effector LARP1. Finally, targeting the Fer driven activation of mTORC1, culminated in necrotic death of the treated PDAC cells, envisaging a new intervention tool for the challenging PDAC disease.
The emerging roles of LINC00511 in breast cancer development and therapy
Authors: Zhao Lifeng , Biswas Sangita , Li Yepeng , Sooranna Suren Rao
Published: August 2024

Breast cancer (BC) is associated with malignant tumors in women worldwide with persistently high incidence and mortality rates. The traditional therapies including surgery, chemotherapy, radiotherapy and targeted therapy have certain therapeutic effects on BC patients, but acquired drug resistance can lead to tumor recurrence and metastasis. This remains a clinical challenge that is difficult to solve during treatment. Therefore, continued research is needed to identify effective targets and treatment methods, to ultimately implement personalized treatment strategies. Several studies have implicated that the long non-coding RNA LINC00511 is closely linked to the occurrence, development and drug resistance of BC. Here we will review the structure and the mechanisms of action of lnc RNA LINC00511 in various cancers, and then explore its expression and its related regulatory mechanisms during BC. In addition, we will discuss the biological functions and the potential clinical applications of LINC00511 in BC.
Reverse degree-based topological indices study of molecular structure in triangular ϒ-graphyne and triangular ϒ-graphyne chain
Authors: Hakeem Abdul , Katbar Nek Muhammad , Shaikh Hisamuddin , Tolasa Fikadu Tesgera , Abro Oshaque Ali
Published: August 2024

Topological indices are mathematical descriptors of the structure of a molecule that can be used to predict its properties. They are derived from the graph theory, which describes the topology of a molecule and its connectivity. The main objective is mathematical modeling and topological properties of ϒ-graphyne. Current research focuses on two structures made from hexagonal honeycomb graphite lattices named triangular ϒ-graphyne and triangular ϒ-graphyne chains. The authors have simultaneously computed the first and second Reverse Zagreb indices, reverse hyper-Zagreb indices, and their polynomials. This research also derives mathematical closed-form formulas for some of its fundamental degree-based molecular descriptors. Researchers have been trying to synthesize a novel carbon form called Graphyne. For over a decade but with no success. Recently, some researchers have made a breakthrough in generating Carbons elusive allotrope and solved a long-standing problem in carbon materials. This wonder material is created to rival the conductivity of graphene but with control. These results opened new ways of research in the fields of semiconductors, electronics and optics. Furthermore, graphical and tabular results will help to investigate the structure-property relationships in γ-graphyne.
NUOVA OFFICINA ASSERGI: a novel infrastructure for the production of cryogenic and radiopure Si-based photodetectors
Authors: Consiglio L. , Flammini A. , Ianni A. , Marasciulli A. , Panella G. , Pietrofaccia L. , Sablone D. , Tartaglia R.
Published: August 2024

The NUOVA OFFICINA ASSERGI (NOA) is a new facility for the production and integration of large-area silicon photodetectors operating at cryogenic temperatures. Silicon photomultipliers are proving to be a promising technology for next-generation experiments searching for rare events in underground laboratories. New photosensor technology with high performance at cryogenic temperature has been developed by Fondazione Bruno Kessler (FBK) and integrated at Laboratori Nazionali del Gran Sasso (LNGS) into large-area optical units, thus opening the frontiers toward the realization of scalable liquid argon experiments probing dark matter. The massive production of such detectors is now feasible in NOA, a clean room of 421 m2 designed to operate in a radon-free mode. NOA, commissioned and operational at LNGS, hosts the most advanced packaging machines and electronic test facilities for the integration of silicon devices in a dust-controlled environment. The infrastructure layout is split into two experimental areas: one for the production of electronic devices and cryogenic temperature tests and the other for operating with large detector installations. The NOA facility can be operated with a radon abatement system, making it a unique facility for packaging and testing SiPM-based photosensors and for assembling detector components in a radon-free environment. Therefore, NOA supports the deployment of underground experiments at LNGS and the development of new technologies for the search of rare events, such as dark matter and neutrinoless double-beta decay.
Formation mechanism of the U-shaped spectrum based on a simple plasma–dielectric–plasma (PDP) waveguide
Authors: Nie Qiuyue , Wei Guoqiang , Zhang Zhonglin , Zhang Zelin , Chen Peiqi , Ai Xin , Qian Liang , Yan Changshi
Published: August 2024

Manipulating electromagnetic (EM) waves by plasma–dielectric–plasma (PDP) waveguides or plasma array structures presents significant potential in microwave signal processing, such as filtering, signal delay, and EM enhancement or shielding. Owing to the simple structure and easy fabrication, the waveguide with a tooth-shaped resonator has been a strong candidate as a filtering device. Based on our previous work focusing on U-shaped filtering excited by PDP waveguides with a double-teeth structure, in this work, the formation mechanism of a U-shape filtering spectrum is systematically explored by transmission line theory (TLT) with proper field distributions. The results indicate that the U-shape spectrum consists of boundary edges and a filtering stopband. The boundary edges are attributed to Fano-type resonance, and the enhanced destructive interference from double teeth is responsible for the stopband. Such an approach shows a specific and clear mechanism for the generated U-shaped spectrum. In addition, the theoretical analysis of double teeth without Fano-type resonances is rigorously demonstrated using TLT, which significantly contributes to bandwidth modulation of stopband filtering in theory. These results contribute to the understanding of the formation mechanism of a U-shaped spectrum from a gap plasmon waveguide (such as PDP or metal–insulator–metal (MIM)) with tooth-shaped resonators, offering a feasible direction for the optimization of filtering properties, as well as offering significant parameters for subsequent experimental design.
Fundamental equations and hypotheses governing glomerular hemodynamics
Authors: Kuang Serena Y. , Ahmetaj Besjana , Qu Xianggui
Published: August 2024

The glomerular filtration rate (GFR) is the outcome of glomerular hemodynamics, influenced by a series of parameters: renal plasma flow, resistances of afferent arterioles and efferent arterioles (EAs), hydrostatic pressures in the glomerular capillary and Bowman’s capsule, and plasma colloid osmotic pressure in the glomerular capillary. Although mathematical models have been proposed to predict the GFR at both the single-nephron level and the two-kidney system level using these parameters, mathematical equations governing glomerular filtration have not been well-established because of two major problems. First, the two-kidney system-level models are simply extended from the equations at the single-nephron level, which is inappropriate in epistemology and methodology. Second, the role of EAs in maintaining the normal GFR is underappreciated. In this article, these two problems are concretely elaborated, which collectively shows the need for a shift in epistemology toward a more holistic and evolving way of thinking, as reflected in the concept of the complex adaptive system (CAS). Then, we illustrate eight fundamental mathematical equations and four hypotheses governing glomerular hemodynamics at both the single-nephron and two-kidney levels as the theoretical foundation of glomerular hemodynamics. This illustration takes two steps. The first step is to modify the existing equations in the literature and establish a new equation within the conventional paradigm of epistemology. The second step is to formulate four hypotheses through logical reasoning from the perspective of the CAS (beyond the conventional paradigm). Finally, we apply the new equation and hypotheses to comprehensively analyze glomerular hemodynamics under different conditions and predict the GFR. By doing so, some concrete issues are eliminated. Unresolved issues are discussed from the perspective of the CAS and a desinger’s view. In summary, this article advances the theoretical study of glomerular dynamics by 1) clarifying the necessity of shifting to the CAS paradigm; 2) adding new knowledge/insights into the significant role of EAs in maintaining the normal GFR; 3) bridging the significant gap between research findings and physiology education; and 4) establishing a new and advanced foundation for physiology education.
NH3/NH4+ allosterically activates SLC4A11 by causing an acidic shift in the intracellular pK that governs H+(OH−) conductance
Authors: Pasternack Richard A. , Quade Bianca N. , Marshall Aniko , Parker Mark D.
Published: August 2024

SLC4A11 is the most abundant membrane transport protein in corneal endothelial cells. Its functional presence is necessary to support the endothelial fluid pump that draws fluid from the corneal stroma, preventing corneal edema. Several molecular actions have been proposed for SLC4A11 including H2O transport and cell adhesion. One of the most reproduced actions that SLC4A11 mediates is a H+ (or OH−) conductance that is enhanced in the presence of NH4Cl. The mechanism by which this occurs is controversial with some providing evidence in favor of NH3-H+ cotransport and others providing evidence for uncoupled H+ transport that is indirectly stimulated by the effects of NH4Cl upon intracellular pH and membrane potential. In the present study we provide new evidence and revisit previous studies, to support a model in which NH4Cl causes direct allosteric activation of SLC4A11 by means of an acidic shift in the intracellular pK (pKi) that governs the relationship between intracellular pH (pHi) and SLC4A11 H+-conductance. These findings have important implications for the assignment of a physiological role for SLC4A11.
Overexpression of the ribosome-inactivating protein OsRIP1 modulates the jasmonate signaling pathway in rice
Authors: Chen Simin , De Zutter Noémie , Meijer Anikó , Gistelinck Koen , Wytynck Pieter , Verbeke Isabel , Osterne Vinicius J. S. , Kondeti Subramanyam , De Meyer Tim , Audenaert Kris , Van Damme Els J. M.
Published: August 2024

Ribosome-inactivating proteins (RIPs) are plant enzymes that target the rRNA. The cytoplasmic RIP, called OsRIP1, plays a crucial role in regulating jasmonate, a key plant hormone. Understanding the role of OsRIP1 can provide insights into enhancing stress tolerance and optimizing growth of rice. Transcription profiling by mRNA sequencing was employed to measure the changes in gene expression in rice plants in response to MeJA treatment. Compared to wild type (WT) plants, OsRIP1 overexpressing rice plants showed a lower increase in mRNA transcripts for genes related to jasmonate responses when exposed to MeJA treatment for 3 h. After 24 h of MeJA exposure, the mRNA transcripts associated with the gibberellin pathway occurred in lower levels in OsRIP1 overexpressing plants compared to WT plants. We hypothesize that the mechanism underlying OsRIP1 antagonization of MeJA-induced shoot growth inhibition involves cytokinin-mediated leaf senescence and positive regulation of cell cycle processes, probably via OsRIP1 interaction with 40S ribosomal protein S5 and α-tubulin. Moreover, the photosystem II 10kDa polypeptide was identified to favorably bind to OsRIP1, and its involvement may be attributed to the reduction of photosynthesis in OsRIP1-overexpressing plants subjected to MeJA at the early timepoint (3 h).
Temporal transcriptome and metabolome study revealed molecular mechanisms underlying rose responses to red spider mite infestation and predatory mite antagonism
Authors: Cai Yanfei , Shi Ziming , Zhao Peifei , Yang Yingjie , Cui Yinshan , Tian Min , Wang Jihua
Published: August 2024

Introduction

Red spider mite (Tetranychus urticae) infestation (SMI) is a detrimental factor for roses grown indoors. Although predatory mite (Neoseiulus californicus) antagonism (PMA) is often utilized to alleviate SMI damage, little is known about the defensive response of greenhouse-grown roses to SMI and the molecular mechanism by which PMA protects roses.

Methods

To determine the transcriptome and metabolome responses of roses to SMI and PMA, the leaves of a rose cultivar (“Fairy Zixia/Nightingale”) were infested with T. urticae, followed by the introduction of predator mite. Leaf samples were collected at various time points and subjected to transcriptome and metabolome analyses.

Results

We found that 24 h of SMI exerted the most changes in the expression of defense-related genes and metabolites in rose leaves. KEGG pathway analysis of differentially expressed genes (DEGs) and metabolites revealed that rose responses to SMI and PMA were primarily enriched in pathways such as sesquiterpenoid and triterpenoid biosynthesis, benzoxazinoid biosynthesis, stilbenoid, diarylheptanoid and gingerol biosynthesis, phytosterol biosynthesis, MAPK signaling pathway, phenylpropanoid biosynthesis, and other pathways associated with resistance to biotic stress. Rose reacted to SMI and PMA by increasing the expression of structural genes and metabolite levels in phytosterol biosynthesis, mevalonate (MVA) pathway, benzoxazinoid biosynthesis, and stilbenoid biosynthesis. In addition, PMA caused a progressive recover from SMI, allowing rose to revert to its normal growth state. PMA restored the expression of 190 essential genes damaged by SMI in rose leaves, including transcription factors DRE1C, BH035, MYB14, EF110, WRKY24, NAC71, and MY108. However, after 144 h of PMA treatment, rose responsiveness to stimulation was diminished, and after 192 h, the metabolic levels of organic acids and lipids were recovered in large measure.

Conclusion

In conclusion, our results offered insights on how roses coordinate their transcriptome and metabolome to react to SMI and PMA, therefore shedding light on how roses, T. urticae, and N. californicus interact.
Impact of fertilization depth on sunflower yield and nitrogen utilization: a perspective on soil nutrient and root system compatibility
Authors: Ren Wenhao , Li Xianyue , Liu Tingxi , Chen Ning , Xin Maoxin , Liu Bin , Qi Qian , Li Gendong
Published: August 2024

Introduction

The depth of fertilizer application significantly influences soil nitrate concentration (SNC), sunflower root length density (RLD), sunflower nitrogen uptake (SNU), and yield. However, current studies cannot precisely capture subtle nutrient variations between soil layers and their complex relationships with root growth. They also struggle to assess the impact of different fertilizer application depths on sunflower root development and distribution as well as their response to the spatial and temporal distribution of nutrients.

Methods

The Agricultural Production Systems sIMulator (APSIM) model was employed to explore the spatial and temporal patterns of nitrogen distribution in the soil at three controlled-release fertilizer (CRF) placement depths: 5, 15, and 25 cm. This study investigated the characteristics of the root system regarding nitrogen absorption and utilization and analyzed their correlation with sunflower yield formation. Furthermore, this study introduced the modified Jaccard index (considering the compatibility between soil nitrate and root length density) to analyze soil-root interactions, providing a deeper insight into how changes in CRF placement depth affect crop growth and nitrogen uptake efficiency.

Results

The results indicated that a fertilization depth of 15 cm improved the modified Jaccard index by 6.60% and 7.34% compared to 5 cm and 25 cm depths, respectively, maximizing sunflower yield (an increase of 9.44%) and nitrogen absorption rate (an increase of 5.40%). This depth promoted a greater Root Length Density (RLD), with an increases of 11.95% and 16.42% compared those at 5 cm and 25 cm, respectively, enhancing deeper root growth and improving nitrogen uptake. In contrast, shallow fertilization led to higher nitrate concentrations in the topsoil, whereas deeper fertilization increased the nitrate concentrations in the deeper soil layers.

Discussion

These results provide valuable insights for precision agriculture and sustainable soil management, highlighting the importance of optimizing root nitrogen absorption through tailored fertilization strategies to enhance crop production efficiency and minimize environmental impact.
“I feel like a fish out of water”: interpreting the occupational stress and well-being experiences of professional classical musicians
Authors: Willis Simone , Mellick Mikel , Neil Rich , Wasley David
Published: August 2024

Introduction

Professional classical musicians operate within a highly demanding environment, which includes organizational, social, and emotional demands. When not effectively coped with, these demands may cause stress and negatively impact well-being. This qualitative study explored the perceived stress and well-being experiences of professional classical musicians through a transactional theory of stress. The study employed a double hermeneutic interpretation of the lived experiences of the perceived demands faced, stress appraisals made, resources used, and the influence on well-being.

Methods

Six professional classical musicians were purposefully selected for participation. Semi-structured interviews were conducted and participants reflected on two events: one they perceived as a positive experience and one that was negative. Transcripts were analyzed using Interpretative Phenomenological Analysis and Group Experiential Themes emerged.

Results

Three Group Experiential Themes were identified: (a) Performance Demands; (b) Organizational Demands; and, (c) Relationship Demands. Participants predominantly appraised demands as a threat. A small number of demands were appraised as a challenge or benefit, and the fewest demands were appraised as causing harm or loss. Participants’ appraisals were informed by underlying properties of stress appraisal such as self and other comparison, and preparation. Participants often relied on personal resources as opposed to available workplace resources. They perceived well-being to relate to stress appraisals with participants experiencing acute and long-term outcomes.

Discussion

This study offers insight into the lived experience of the occupational stress process within professional classical musicians. The findings demonstrate that organizational interventions targeted at continuing professional development and social support are appropriate to help musicians cope more effectively with demands.
Bulimia nervosa and treatment-related disparities: a review
Authors: Wilson Kim , Kagabo Robert
Published: August 2024

Introduction

Bulimia nervosa (BN) is a type of eating disorder disease usually manifesting between adolescence and early adulthood with 12 as median age of onset. BN is characterized by individuals’ episodes of excessive eating of food followed by engaging in unusual compensatory behaviors to control weight gain in BN. Approximately 94% of those with BN never seek or delay treatment. While there are available treatments, some populations do not have access. Left untreated, BN can become severe and lead to other serious comorbidities. This study is a review of randomized controlled trials to explore available treatments and related treatment disparities. The objective of this review was to identify differences among treatment modalities of BN and aide in the further treatment and research of bulimia nervosa.

Methods

This study followed narrative overview guidelines to review BN treatment studies published between 2010 and 2021. The authors used PubMed and PsychInfo databases to search for articles meeting the inclusion criteria. Search terms included phrases such as, BN treatment, BN and clinical trials, and BN and randomized clinical trials.

Results

Most of the reviewed studies had their sample sizes between 80 and 100% female with age range between 18 and 60 years old. Sample sizes were mostly between 80 and 100% white. Treatment practices included both pharmacological and psychosocial interventions, such as cognitive behavioral therapy (CBT) and limited motivational interviewing (MI). Most studies were in outpatient settings.

Conclusion

Reviewed research shows that certain populations face disparities in BN treatment. Generally, individuals older than 60, males and racial minorities are excluded from research. Researchers and practitioners need to include these vulnerable groups to improve BN treatment-related disparities.
Perceived benefits and limitations of a psychoeducation program for patients with fibromyalgia: an interpretative phenomenological analysis
Authors: Di-Bonaventura Silvia , Ferrer-Peña Raúl , Pardo-Montero Joaquín , Férnandez-Carnero Josué , La Touche Roy
Published: August 2024

Objective

To analyze the perceived benefits and limitations of a pain psychoeducation program as a non-pharmacological treatment for patients with fibromyalgia.

Methods

An interpretative phenomenological analysis was applied to analyze the subjective experiences of 11 patients with fibromyalgia who participated in a pain psychoeducation program. This program includes educational sessions that address pain understanding, coping strategies, and relaxation techniques. Semi-structured interviews were conducted, transcribed, and analyzed using ATLAS.ti software.

Results

Patients reported significant improvements in cognitive-functional capacity and socio-emotional ability, including better disease understanding and management, emotional stability, and interpersonal relationships. Despite these benefits, they identified limitations in program individualization and insufficient coverage of certain topics, such as sexual health and legal aspects of disability. Enhanced self-management skills were evident, with observed shifts in disease perception and coping strategies.

Conclusion

The psychoeducation program was viewed positively, influencing functional, cognitive, and emotional enhancements. Nonetheless, the need for increased program personalization and expanded socio-economic support was noted. Future research should focus on the long-term impacts of psychoeducation and the feasibility of tailored interventions.