bluuebunny/crossref_metadata_2025 huggingface dataset:
HDAC Inhibitors: Innovative Strategies for Their Design and Applications
Authors: Daśko Mateusz , de Pascual-Teresa Beatriz , Ortín Irene , Ramos Ana
Published: January 2022

Histone deacetylases (HDACs) are a large family of epigenetic metalloenzymes that are involved in gene transcription and regulation, cell proliferation, differentiation, migration, and death, as well as angiogenesis. Particularly, disorders of the HDACs expression are linked to the development of many types of cancer and neurodegenerative diseases, making them interesting molecular targets for the design of new efficient drugs and imaging agents that facilitate an early diagnosis of these diseases. Thus, their selective inhibition or degradation are the basis for new therapies. This is supported by the fact that many HDAC inhibitors (HDACis) are currently under clinical research for cancer therapy, and the Food and Drug Administration (FDA) has already approved some of them. In this review, we will focus on the recent advances and latest discoveries of innovative strategies in the development and applications of compounds that demonstrate inhibitory or degradation activity against HDACs, such as PROteolysis-TArgeting Chimeras (PROTACs), tumor-targeted HDACis (e.g., folate conjugates and nanoparticles), and imaging probes (positron emission tomography (PET) and fluorescent ligands).
Four New Benzoylamide Derivatives Isolated from the Seeds of Lepidium apetalum Willd. and Ameliorated LPS-Induced NRK52e Cells via Nrf2/Keap1 Pathway
Authors: Li Meng , Zhang Beibei , Zeng Mengnan , Zhang Jingke , Zhang Zhiguang , Feng Weisheng , Zheng Xiaoke
Published: January 2022

Four new benzoylamide derivatives, lepidiumamide B–E (1–4), were isolated from the seeds of Lepidium apetalum Willd. The structures were determined by a combination of MS and NMR analyses. All compounds were evaluated for their protective effects against NRK-52e cell injury induced by lipopolysaccharide (LPS) in vitro. These compounds showed significantly protective activity and ameliorated LPS-induced NRK52e cells via the Nrf2/Keap1 pathway. The discovery of these active compounds is important for the prevention and treatment of renalinjury.
Advances in Management of Bladder Cancer—The Role of Photodynamic Therapy
Authors: Kubrak Tomasz , Karakuła Michał , Czop Marcin , Kawczyk-Krupka Aleksandra , Aebisher David
Published: January 2022

Photodynamic therapy (PDT) is a non-invasive and modern form of therapy. It is used in the treatment of non-oncological diseases and more and more often in the treatment of various types of neoplasms in various locations including bladder cancer. The PDT method consists of local or systemic application of a photosensitizer, i.e., a photosensitive compound that accumulates in pathological tissue. Light of appropriate wavelength is absorbed by the photosensitizer molecules, which in turn transfers energy to oxygen or initiates radical processes that leads to selective destruction of diseased cells. The technique enables the selective destruction of malignant cells, as the photocytotoxicity reactions induced by the photosensitizer take place strictly within the pathological tissue. PDT is known to be well tolerated in a clinical setting in patients. In cited papers herein no new safety issues were identified. The development of anti-cancer PDT therapies has greatly accelerated over the last decade. There was no evidence of increased or cumulative toxic effects with each PDT treatment. Many modifications have been made to enhance the effects. Clinically, bladder cancer remains one of the deadliest urological diseases of the urinary system. The subject of this review is the anti-cancer use of PDT, its benefits and possible modifications that may lead to more effective treatments for bladder cancer. Bladder cancer, if localized, would seem to be a good candidate for PDT therapy since this does not involve the toxicity of systemic chemotherapy and can spare normal tissues from damage if properly carried out. It is clear that PDT deserves more investment in clinical research, especially for plant-based photosensitizers. Natural PS isolated from plants and other biological sources can be considered a green approach to PDT in cancer therapy. Currently, PDT is widely used in the treatment of skin cancer, but numerous studies show the advantages of related therapeutic strategies that can help eliminate various types of cancer, including bladder cancer. PDT for bladder cancer in which photosensitizer is locally activated and generates cytotoxic reactive oxygen species and causing cell death, is a modern treatment. Moreover, PDT is an innovative technique in oncologic urology.
Enhancing Ocular Bioavailability of Ciprofloxacin Using Colloidal Lipid-Based Carrier for the Management of Post-Surgical Infection
Authors: Al-Joufi Fakhria A. , Salem-Bekhit Mounir M. , Taha Ehab I. , Ibrahim Mohamed A. , Muharram Magdy M. , Alshehri Sultan , Ghoneim Mohammed M. , Shakeel Faiyaz
Published: January 2022

Conjunctivitis and endogenous bacterial endophthalmitis mostly occurred after ophthalmic surgery. Therefore, the present study aimed to maximize the ocular delivery of ciprofloxacin (CPX) using colloidal lipid-based carrier to control the post-surgical infection. In this study, CPX was formulated as ophthalmic liposomal drops. Two different phospholipids in different ratios were utilized, including phosphatidylcholine (PC) and dimyrestoyl phosphatidylcholine (DMPC). The physiochemical properties of the prepared ophthalmic liposomes were evaluated in terms of particle size, entrapment efficiency, polydispersity index, zeta potential, and cumulative CPX in-vitro release. In addition, the effect of sonication time on particle size and entrapment efficiency of CPX ophthalmic drops was also evaluated. The results revealed that most of the prepared formulations showed particle size in nanometer size range (460–1047 nm) and entrapment efficiency ranging from 36.4–44.7%. The antibacterial activity and minimum inhibitory concentration (MIC) were investigated. Ex vivo antimicrobial effect of promising formulations was carried out against the most common causes of endophthalmitis microorganisms. The pharmacokinetics of the prepared ophthalmic drops were tested in rabbit aqueous humor and compared with commercial CPX ophthalmic drops (Ciloxan®). Observed bacterial suppression was detected in rabbit’s eyes conjunctivitis with an optimized formulation A3 compared with the commercial ophthalmic drops. CPX concentration in the aqueous humor was above MIC against tested bacterial strains. The in vivo data revealed that the tested CPX drops showed superiority over the commercial ones with respect to peak aqueous humor concentration, time to reach peak aqueous humor concentration, elimination rate constant, half-life, and relative bioavailability. Based on these results, it was concluded that the prepared ophthalmic formulations significantly enhanced CPX bioavailability compared with the commercial one.
Detection of Rift Valley Fever Virus in Aedes (Aedimorphus) durbanensis, South Africa
Authors: van den Bergh Carien , Thompson Peter N. , Swanepoel Robert , Almeida Antonio P. G. , Paweska Janusz T. , Jansen van Vuren Petrus , Wilson William C. , Kemp Alan , Venter Estelle H.
Published: January 2022

Rift Valley fever virus (RVFV) is a mosquito-borne, zoonotic phlebovirus-causing disease in domestic ruminants and humans in Africa, the Arabian Peninsula and some Indian Ocean islands. Outbreaks, characterized by abortion storms and a high morbidity rate in newborn animals, occur after heavy and prolonged rainfalls favouring the breeding of mosquitoes. However, the identity of the important mosquito vectors of RVFV is poorly known in most areas. Mosquitoes collected in the Ndumo area of tropical north-eastern KwaZulu-Natal (KZN), South Africa, were tested for RVFV nucleic acid using RT-PCR. The virus was detected in a single pool of unfed Aedes (Aedimorphus) durbanensis, indicating that this seasonally abundant mosquito species could serve as a vector in this area of endemic RVFV circulation. Phylogenetic analysis indicated the identified virus is closely related to two isolates from the earliest outbreaks, which occurred in central South Africa more than 60 years ago, indicating long-term endemicity in the region. Further research is required to understand the eco-epidemiology of RVFV and the vectors responsible for its circulation in the eastern tropical coastal region of southern Africa.
New and Confirmed Foci of Tick-Borne Encephalitis Virus (TBEV) in Northern Germany Determined by TBEV Detection in Ticks
Authors: Topp Anna-Katharina , Springer Andrea , Dobler Gerhard , Bestehorn-Willmann Malena , Monazahian Masyar , Strube Christina
Published: January 2022

Tick-borne encephalitis (TBE) is a tick-transmitted, virus-induced neurological disease with potentially fatal outcomes in humans and animals. Virus transmission takes places in so-called tick-borne encephalitis virus (TBEV) microfoci, which constitute small areas of sustained virus circulation. In southern Germany, TBEV has been endemic for decades; however, a northward expansion of risk areas, based on disease incidence in the human population, has been observed in recent years. The present study investigated TBEV occurrence in questing ticks at eight locations in the federal state of Lower Saxony, northwestern Germany, chosen due to reported associations with human TBE cases (N = 4) or previous virus detection (N = 4). A total of 20,056 ticks were collected in 2020 and 2021 and tested for TBEV RNA in pools of ten nymphs or five adults by quantitative reverse transcription-PCR (RT-qPCR). Positive results were confirmed by RT amplification of the viral E gene. In total, 18 pools from five different sampling locations were positive for TBEV RNA. One previously unknown transmission focus was detected, while ongoing virus circulation was confirmed at the four further locations. Phylogenetic analysis showed that two different virus strains with different origins circulate in the locations identified as natural foci.
Virulence Profiles and Antibiotic Susceptibility of Escherichia coli Strains from Pet Reptiles
Authors: Dec Marta , Stepien-Pysniak Dagmara , Szczepaniak Klaudiusz , Turchi Barbara , Urban-Chmiel Renata
Published: January 2022

Exotic reptiles are increasingly being bred as pets in many countries around the world, including Poland. However, the close contact between reptiles and their owners provides favourable conditions for the transmission of zoonotic pathogens. In this work, we examined E. coli isolates from 67 captive reptiles regarding their virulence, antibiotic susceptibility, phylogenetic affiliation, and genetic diversity. The incidence of E. coli was highest in snakes (51.6%, 16 isolates/31 samples), and slightly lower in turtles (44.4%, 8/18) and lizards (44.4%, 8/18). Genes encoding virulence factors were confirmed in 50% of isolates and the most common were the traT (37.5%, n = 12), fyuA (21.87%, n = 7), and irp-2 (15.62%, n = 5). The majority (71.87%, n = 23) of E. coli isolates were susceptible to all of the antimicrobial substances used in the study. Streptomycin resistance (21.87%, n = 7) was the most frequent, while resistance to other antimicrobial substances was sporadic. One strain (3.12%) was classified as multidrug-resistant. The presence of resistance genes (aadA, tetA, tetB, tetM, and blaTEM) was confirmed in 12.5% (n = 4) of the isolates. The majority (65.6%, n = 21) of E. coli isolates represented the B1 phylogenetic group. (GTG)5-PCR fingerprinting showed considerable genetic variation in the pool of tested isolates. The frequency of E. coli in reptiles is much lower than in mammals or birds. Due to the presence of virulence genes, characteristic of both intestinal pathogenic E. coli (IPEC) and extraintestinal pathogenic E. coli (ExPEC), reptilian strains of E. coli have pathogenic potential, and therefore people in contact with these animals should follow good hygiene practices.
African Swine Fever Survey in a European Context
Authors: de la Torre Ana , Bosch Jaime , Sánchez-Vizcaíno José Manuel , Ito Satoshi , Muñoz Carolina , Iglesias Irene , Martínez-Avilés Marta
Published: January 2022

African swine fever (ASF) is currently the most threatening disease for domestic and wild pigs worldwide. Wild boar has been the main affected species in all EU countries except for Romania, where most notifications occur in domestic pigs. The spread of ASF in wild boar is challenging to control; risk factors are harder to identify and establish than in domestic pigs, which, together with an underestimation of the disease and the lack of treatment or an effective vaccine, are hindering control and eradication efforts. We distributed two online questionnaires, one for domestic pigs and one for wild boar, to experts of different background and countries in Europe, to explore risk factors in relation to ASF control connected to farming, hunting, trade, the environment, and domestic pig and wild boar populations. Overall, wild boar movements were estimated to pose the highest risk of ASF introduction and spread. The movement of pork and pork products for own consumption also ranked high. Here we explored, in addition to the assessment of risk pathways, the identification of risks of transmission at the domestic/wild boar interface, the importance of biosecurity practices and improved control efforts, and controversial opinions that require further attention.
Evaluation of a Lateral Flow Assay for Rapid Detection of African Swine Fever Virus in Multiple Sample Types
Authors: Onyilagha Chukwunonso , Nguyen Kelvin , Luka Pam D. , Hussaini Ularamu , Adedeji Adeyinka , Odoom Theophilus , Ambagala Aruna
Published: January 2022

Antibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect pathogens in field samples, especially in hard-to-reach remote areas that may have limited access to central laboratories during an outbreak or surveillance. In this study, we investigated the ability of a commercially available LFA, PenCheck®, to detect African swine fever virus (ASFV) in clinical samples derived from pigs infected with highly virulent ASFV strains. The assay was specific and positively identified the majority of pigs showing high fever during the early stages (between 3 and 5 days) of infection. PenCheck® LFA also detected ASFV in serum and tissue samples collected from pigs that succumbed to experimental ASFV infection and whole blood, plasma, and tissue samples from the field. The limit of detection of the assay was ASFV titer 107.80 TCID50/mL, corresponding to ASFV real-time PCR values below 23 Ct. Although the sensitivity of the assay is less than that of the laboratory-based real-time PCR assays, the results obtained with the PenCheck® LFA in this study suggest that it can be used as a herd-level, field-deployable, and easy-to-use diagnostic tool to identify ASF-affected farms when access to portable molecular assays or central laboratories is not possible.
Deontological Desert
Authors: Kagan Shelly
Published: January 2022

Although the nature of moral desert has sometimes been examined in axiological terms—focusing on the thought that it is a good thing if people get what they deserve—deontologists typically think desert is more appropriately treated in terms of duties and obligations. They may, for example, prefer to talk in terms of there being a moral duty to give people what they deserve. This essay distinguishes a number of forms such a duty might take, and examines four of them more closely. (In particular, it looks at positive and negative duties with regard to both comparative and noncomparative desert). Questions about the contents of each of these duties are raised, making clearer just how much work would be involved in spelling out the relevant duties more completely. The essay concludes with a brief discussion of the possible implications of such desert-based duties for population ethics.
On the Limits of Across-the-Board Movement: Distributed Extraction Coordinations
Authors: Bošković Željko
Published: January 2022

The paper examines distributed extraction coordinations, in which different elements move out of conjuncts of a single coordination, as in Which book and which magazine did Mary buy and Amy read respectively, from a crosslinguistic perspective. A number of properties of such coordinations are discussed, which includes showing that they are also subject to the ATB requirement, which will shed light on the nature of the ATB phenomenon itself. It is also shown that there is a rather strong restriction on distributed extractions which confines such extractions to one context and completely excludes one type of movement, in particular head-movement, from participating in them. The higher coordination is shown to be formed during the derivation and to be semantically expletive. Distributed extraction constructions are also shown to have consequences for the proper analysis of a number of phenomena, including subject-oriented anaphors, right node raising, tough-constructions, agreement, and clausal structure. Regarding subject-oriented anaphors, the paper teases apart different approaches to subject-oriented anaphors based on constructions where different elements fill SpecvP and SpecTP (the latter undergoes agreement with T and the former binds subject-oriented anaphors).
Characterization of an Arabidopsis Defensin-like Gene Conferring Resistance against Nematodes
Authors: Hawamda Abdalmenem I. M. , Reichert Susanne , Ali Muhammad Amjad , Nawaz Muhammad Amjad , Austerlitz Tina , Schekahn Patricia , Abbas Amjad , Tenhaken Raimund , Bohlmann Holger
Published: January 2022

Arabidopsis contains 317 genes for defensin-like (DEFL) peptides. DEFLs have been grouped into different families based mainly on cysteine motifs. The DEFL0770 group contains seven genes, of which four are strongly expressed in roots. We found that the expression of these genes is downregulated in syncytia induced by the beet cyst nematode Heterodera schachtii as revealed by RNAseq analysis. We have studied one gene of this group, At3g59930, in detail. A promoter::GUS line revealed that the gene is only expressed in roots but not in other plant organs. Infection of the GUS line with larvae of H. schachtii showed a strong downregulation of GUS expression in infection sites as early as 1 dpi, confirming the RNAseq data. The At3g59930 peptide had only weak antimicrobial activity against Botrytis cinerea. Overexpression lines had no enhanced resistance against this fungus but were more resistant to H. schachtii infection. Our data indicate that At3g59930 is involved in resistance to nematodes which is probably not due to direct nematicidal activity.
High-Purity Preparation of Enzyme Transformed Trans-Crocetin Reclaimed from Gardenia Fruit Waste
Authors: Chyau Charng-Cherng , Chiu Chu-Ying , Hsieh Hung-Lin , Hsieh David Wang-Chi , Hsieh Chong-Ru , Chang Chi-Huang , Peng Robert Y.
Published: January 2022

The recovery of physiologically bioactive ingredients from agricultural wastes as an abundant and low-cost source for the production of high value-added mutraceuticlas has been recognized and supported for the commercial interests and sustainable managements. In the extraction of geniposide for the development of natural food colorants from the dried fruits of Gardenia jasminoides Rubiaceae, the gardenia fruit waste (GFW) still remaining 0.86% (w/w) of crocins has always been discarded without any further treatments Until now, there was no simple and effective protocol for high-purity trans-crocein (TC) preparation without the coexistence of non-biologically active cis-crocein from GFW. We proposed an effective process to obtain the compound as follows. Crocins were extracted firstly by 50% of ethanol in the highest yield of 8.61 mg/g (w/w) from GFW. After the HPD-100 column fractionation in the collecting of crocins, the conversion ratio of 75% of crocins to crocetins can be obtained from the commercial available enzyme- Celluclast® 1.5 L. The crocins hydrolyzed products, were then separated through the HPD-100 resin adsorption and finally purified with the centrifugal partition chromatography (CPC) in single-step to obtain TC in a purity of 96.76 ± 0.17%. Conclusively, the effective enzyme transformation and purification co-operated with CPC technologies on crocins resulted in a high purity product of TC may be highly application in the commercial production.
A Beneficial Plant-Associated Fungus Shifts the Balance toward Plant Growth over Resistance, Increasing Cucumber Tolerance to Root Herbivory
Authors: Rivera-Vega Loren J. , Grunseich John M. , Aguirre Natalie M. , Valencia Cesar U. , Sword Gregory A. , Helms Anjel M.
Published: January 2022

Plants allocate their limited resources toward different physiological processes, dynamically adjusting their resource allocation in response to environmental changes. How beneficial plant-associated microbes influence this allocation is a topic that continues to interest plant biologists. In this study, we examined the effect of a beneficial fungus, Phialemonium inflatum, on investment in growth and anti-herbivore resistance traits in cucumber plants (Cucumis sativus). We inoculated cucumber seeds with P. inflatum spores and measured several growth parameters, including germination rate, above and belowground biomass, and number of flowers. We also examined plant resistance to adult and larval striped cucumber beetles (Acalymma vitattum), and quantified levels of defense hormones in leaves and roots. Our results indicate that P. inflatum strongly enhances cucumber plant growth and reproductive potential. Although fungus treatment did not improve plant resistance to cucumber beetles, inoculated plants were more tolerant to root herbivory, experiencing less biomass reduction. Together, these findings document how a beneficial plant-associated fungus shifts plant investment in growth over herbivore resistance, highlighting the importance of microbes in mediating plant-herbivore interactions. These findings also have important implications for agricultural systems, where beneficial microbes are often introduced or managed to promote plant growth or enhance resistance.
Genotype and Phenotype Interaction between OsWKRYq6 and BLB after Xanthomonas oryzae pv. Oryzae Inoculation in the Field
Authors: Du Xiao-Xuan , Park Jae-Ryoung , Wang Xiao-Han , Jan Rahmatullah , Lee Gang-Seob , Kim Kyung-Min
Published: January 2022

Bacterial leaf blight (BLB) is an important and devastating rice disease caused by the pathogen Xanthomonas oryzae pv. Oryzae (Xoo). In particular, in recent years, the occurrence of abnormal climate and warming phenomena has produced a good environment for the occurrence of BLB, and the rice yield due to the occurrence of BLB continues to decrease. Currently, molecular breeding is applied by searching for resistant genes to development of BLB resistance cultivar. In addition, there are many methods for screening resistant genes, and among them, phenotype analysis in the field and applied research is rarely conducted. Due to recent rapid climate change, BLB is a major problem that has a more serious negative effect on rice yield. Therefore, we suggest OsWRKYq6 to be effectively used for breeding BLB-resistant cultivars by screening BLB-resistant genes. In this study, the BLB-resistant gene was screened using the lesion length, which most definitely changes to the phenotype when Xoo is infected. OsWRKYq6 was finally selected as a BLB resistance gene by analyzing the phenotype and genotype after inoculating Xoo in 120 Cheongcheong/Nagdong double haploid (CNDH) lines in the field. After Xoo inoculation, lesion length and yield were investigated, and 120 CNDH lines were divided from BLB-resistant and susceptible lines. Moreover, when the transcription level of OsWRKYq6 was analyzed in the resistant and susceptible lines after Xoo inoculation in the field, the expression level was regulated to a high level in the resistant line. In this study, we propose OsWRKYq6 as a transcription factor involved in BLB resistance. Currently, the differentiation of various races is proceeding rapidly due to rapid climate change. In addition, screening of transcription factor genes involved in BLB resistance in the field can be effectively applied to molecular breeding to develop resistant cultivars in preparation for rapid climate change.
Deciphering Molecular Mechanisms Involved in Salinity Tolerance in Guar (Cyamopsis tetragonoloba (L.) Taub.) Using Transcriptome Analyses
Authors: Acharya Biswa R. , Sandhu Devinder , Dueñas Christian , Ferreira Jorge F. S. , Grover Kulbhushan K.
Published: January 2022

Guar is a commercially important legume crop known for guar gum. Guar is tolerant to various abiotic stresses, but the mechanisms involved in its salinity tolerance are not well established. This study aimed to understand molecular mechanisms of salinity tolerance in guar. RNA sequencing (RNA-Seq) was employed to study the leaf and root transcriptomes of salt-tolerant (Matador) and salt-sensitive (PI 340261) guar genotypes under control and salinity. Our analyses identified a total of 296,114 unigenes assembled from 527 million clean reads. Transcriptome analysis revealed that the gene expression differences were more pronounced between salinity treatments than between genotypes. Differentially expressed genes associated with stress-signaling pathways, transporters, chromatin remodeling, microRNA biogenesis, and translational machinery play critical roles in guar salinity tolerance. Genes associated with several transporter families that were differentially expressed during salinity included ABC, MFS, GPH, and P-ATPase. Furthermore, genes encoding transcription factors/regulators belonging to several families, including SNF2, C2H2, bHLH, C3H, and MYB were differentially expressed in response to salinity. This study revealed the importance of various biological pathways during salinity stress and identified several candidate genes that may be used to develop salt-tolerant guar genotypes that might be suitable for cultivation in marginal soils with moderate to high salinity or using degraded water.
Impact of Drying Processes on Phenolics and In Vitro Health-Related Activities of Indigenous Plants in Thailand
Authors: Sirichai Pandaree , Kittibunchakul Suwapat , Thangsiri Sirinapa , On-Nom Nattira , Chupeerach Chaowanee , Temviriyanukul Piya , Inthachat Woorawee , Nuchuchua Onanong , Aursalung Amornrat , Sahasakul Yuraporn , Charoenkiatkul Somsri , Suttisansanee Uthaiwan
Published: January 2022

Thailand has vast areas of tropical forests with many indigenous plants, but limited information is available on their phytochemical profile and in vitro inhibitions of enzymatic and nonenzymatic reactions. This study investigated phenolic profiles using liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS), antioxidant activities, and in vitro inhibitory activities of 10 indigenous plants on key enzymes related to obesity (lipase), diabetes (α-amylase and α-glucosidase), and Alzheimer’s disease (cholinesterases and β-secretase). The nonenzymatic anti-glycation reaction was also investigated. The 10 indigenous plants were Albizia lebbeck (L.) Benth, Alpinia malaccensis (Burm.) Roscoe, Careya arborea Roxb., Diplazium esculentum (Retz.) Swartz, Kaempferia roscoeana Wall., Millettia brandisiana Kurz., Momordica charantia, Phyllanthusemblica L., Zingiber cassumunar Roxb, and Zingiber citriodorum J. Mood & T. Theleide. Preparations were made by either freeze-drying or oven-drying processes. Results suggested that the drying processes had a minor impact on in vitro inhibitions of enzymatic and nonenzymatic reactions (<4-fold difference). P. emblica was the most potent antioxidant provider with high anti-glycation activity (>80% inhibition using the extract concentration of ≤6 mg/mL), while D. esculentum effectively inhibited β-secretase activity (>80% inhibition using the extract concentration of 10 mg/mL). C. arborea exhibited the highest inhibitory activities against lipase (47–51% inhibition using the extract concentration of 1 mg/mL) and cholinesterases (>60% inhibition using the extract concentration of 2 mg/mL), while Mi. brandisiana dominantly provided α-amylase and α-glucosidase inhibitors (>80% inhibition using the extract concentration of ≤2 mg/mL). Information obtained from this research may support usage of the oven-drying method due to its lower cost and easier preparation step for these studied plant species and plant parts. Furthermore, the information on in vitro inhibitions of enzymatic and nonenzymatic reactions could be used as fundamental knowledge for further investigations into other biological activities such as cell culture or in vivo experiments of these health-beneficial plants.
D-Tagatose-Based Product Triggers Sweet Immunity and Resistance of Grapevine to Downy Mildew, but Not to Gray Mold Disease
Authors: Mijailovic Nikola , Richet Nicola , Villaume Sandra , Nesler Andrea , Perazzolli Michele , Aït Barka Essaid , Aziz Aziz
Published: January 2022

The use of natural bio-based compounds becomes an eco-friendly strategy to control plant diseases. Rare sugars would be promising compounds as inducers of plant “sweet immunity”. The present study aimed to investigate the induced resistance of grapevine leaves against Plasmopara viticola and Botrytis cinerea by a rare sugar-based product (IFP48) and its active ingredient D-tagatose (TAG), in order to elucidate molecular mechanism involved in defense-related metabolic regulations before and after pathogen challenge. Data showed that spraying leaves with IFP48 and TAG lead to a significant reduction of downy mildew, but not of gray mold disease. The induced protection against P. viticola relies on IFP48’s and to a lesser extent TAG’s ability to potentiate the activation of salicylic acid- and jasmonic acid/ethylene-responsive genes and stilbene phytoalexin accumulation. Most of defense responses remained upregulated in IFP48-treated plants after infection with P. viticola, but inconsistent following challenge with B. cinerea. The beneficial effects of IFP48 were associated with an enhanced accumulation of tagatose inside leaf tissues compared to TAG treatment. Meanwhile, the amounts of sugars, glucose, fructose, maltose, galactose and trehalose remained unchanged or decreased in IFP48-treated leaves after P. viticola infection, although only a few genes involved in sugar transport and metabolism showed transcriptional regulation. This suggests a contribution of sugar homeostasis to the IFP48-induced sweet immune response and priming plants for enhanced resistance to P. viticola, but not to B. cinerea.
Metal Additive Manufacturing of Plastic Injection Molds with Conformal Cooling Channels
Authors: Kanbur Baris Burak , Zhou Yi , Shen Suping , Wong Kim Hai , Chen Charles , Shocket Abe , Duan Fei
Published: January 2022

Conformal cooling channels (CCCs) are widely used in the plastic injection molding process to improve the product quality and operational performance. Tooling that incorporates CCCs can be fabricated through metal additive manufacturing (MAM). The present work focuses on the MAM of a plastic injection mold insert with different CCC types that are circular, serpentine, and tapered channels with/without body-centered cubic (BCC) lattices. The entire manufacturing process of the mold insert is explained from the design step to the final printing step including the computational thermal & mechanical simulations, performance assessments, and multiobjective optimization. Compared to the traditional channels, conformal cooling channels achieved up to 62.9% better cooling performance with a better thermal uniformity on the mold surface. The optimum mold geometry is decided using the multiobjective optimization procedure according to the multiple objectives of cooling time, temperature non-uniformity, and pressure drop in the channel. Direct Metal Laser Sintering (DMLS) method is used for manufacturing the molds and the quality of the printed molds are analyzed with the X-ray Computed Tomography (X-ray CT) technique. The errors between the design and the printed parameters are less than 5% for the circular and tapered channels while the maximum deviation of the strut diameters of the BCC is 0.06 mm.
Review of the Performance of High-Voltage Composite Insulators
Authors: Saleem Muhammad Zaheer , Akbar Mohammad
Published: January 2022

In the present literature survey, we focused on the performance of polymeric materials encompassing silicone rubber (SiR), ethylene propylene diene monomer (EPDM) and epoxy resins loaded with micro, nano, and micro/nano hybrid fillers. These insulators are termed as composite insulators. The scope of the added fillers/additives was limited to the synthetic inorganic family. Special attention was directed to understanding the effect of fillers on the improvement of the thermal conductivity, dielectric strength, mechanical strength, corona discharge resistance, and tracking and erosion resistance performance of polymeric materials for use as high-voltage transmission line insulators. The survey showed that synthetic inorganic fillers, which include silica (SiO2) and hexagonal boron nitride (h-BN), are potential fillers to improve insulation performance of high-voltage insulators. Furthermore, nano and micro/nano filled composites performed better due to the better interaction between the filler and polymer matrix as compared to their only micro- or nano filled counterparts. Finally, some aspects requiring future work to further exploit fillers are identified and discussed.
Growth Enhancement of Camellia sinensis with Biochar
Authors: Sipayung Herta Novalina , Wu Keng-Tung , Liu De-Yu , Chen Chien-Teh
Published: January 2022

Biochar application in the agriculture sector is a practical approach to minimize waste and maintain sustainable farming. However, the information regarding biochar application in tea cultivation is limited and especially rare in field research. In this study, a two-year biochar experiment was conducted in a tea yard subject to tea yield and soil characteristics. According to tea growth, the tea yard for the experiment was divided into weak and normal sectors. Biochar was made from tea processing waste, mainly the twigs. The results showed that biochar application increased tea production by 2.3- and 1.3-fold in the weak and normal sectors, respectively, suggesting that converting tea waste to biochar for soil amendment is beneficial to increasing tea production, particularly in the weak sector. The regression analysis on tea production and soil characteristics revealed that only soil electric conductivity (R2 = 0.6597), soil organic matter (R2 = 0.7024), and soil extractable phosphate (R2 = 0.6547) were positively correlated with tea yield. Therefore, the field experiment of this study improves the understanding of biochar’s agronomic and environmental benefits on plant productivity and soil health.
Effects of Some Weak Acids and Moringa oleifera Leaf Extract Powder on the Colour of Dried Apple
Authors: Arendse Washiela , Jideani Victoria
Published: January 2022

This study aimed to find alternatives to sulphite as a preservative for dried fruits. Granny Smith apples were sanitised in a 200 ppm sodium chlorite solution, de-cored, peeled, and cut into slices. The sliced apples were pre-treated/dipped in a water solution containing the three weak acids, namely, ascorbic acid (AA), citric acid (CA), and potassium sorbate (PS) as well as Moringa oleifera leaf extract powder (MOLEP). A screening fractional factorial experiment consisting of five independent variables (AA, CA and PS, time and temperature and MOLEP) constrained at their upper and lower levels (AA: 0.5 to 2.0%, CA: 0.3 to 2.0%, MOLEP: 0.1 to 0.2%, time: 7 to 15 h and temperature: 57 to 70 °C) were evaluated for their effect on the colour of the dried sliced apples. An increase in the concentration of the CA significantly increased the lightness (p = 0.05) and decreased the redness (p = 0.0022) of the dried apple slices. AA and PS did not impact the lightness of the dried sliced apples. A dipping solution of citric acid at 2.0%, Moringa oleifera leaf extract powder at 0.1%, and drying time of 7 h at 70 °C effectively minimized the discolouration of the dried sliced apples.
The Open Well as Symbol of the Meeting of Heaven and Earth
Authors: Sahi Jyoti
Published: January 2022

As an Indian artist working in the field of cross-cultural visual theology, I have taken the open well as a common feature of the landscape where I live as a motif that I can connect with the biblical story of Jesus meeting the woman at the well. This leads to a discussion around the symbolic significance of the water of life. The landscape provides natural elements that are both particular and local but also universal in their cultural significance. The meeting between the thirsty traveler, who is Christ the teacher, and a socially marginalized woman who comes to draw water in the midday heat provides the occasion for a dialogue in the context of asking for water. There is a similar story in the Buddhist tradition where Ananda, the disciple of Buddha, meets with an ‘outcaste’ woman at a well. Water, which is always found at a lowly place, becomes a symbol for the socially depressed. What is below must be lifted if the living water is to renew and transform the searcher. The encounter at the well can become the basis for a dialogue between religions concerning the need for social inclusion.
A VLSI Chip for the Abnormal Heart Beat Detection Using Convolutional Neural Network
Authors: Chen Yuan-Ho , Chen Szi-Wen , Chang Pei-Jung , Hua Hsin-Tung , Lin Shinn-Yn , Chen Rou-Shayn
Published: January 2022

The heart is one of the human body’s vital organs. An electrocardiogram (ECG) provides continuous tracings of the electrophysiological activity originated from heart, thus being widely used for a variety of diagnostic purposes. This study aims to design and realize an artificial intelligence (AI)-based abnormal heart beat detection with applications for early detection and timely treatment for heart diseases. A convolutional neural network (CNN) was employed to achieve a fast and accurate identification. In order to meet the requirements of the modularity and scalability of the circuit, modular and efficient processing element (PE) units and activation function modules were designed. The proposed CNN was implemented using a TSMC 0.18 μm CMOS technology and had an operating frequency of 60 MHz with chip area of 1.42 mm2 and maximum power dissipation of 4.4 mW. Furthermore, six types of ECG signals drawn from the MIT-BIH arrhythmia database were used for performance evaluation. Results produced by the proposed hardware showed that the discrimination rate was 96.3% with high efficiency in calculation, suggesting that it may be suitable for wearable devices in healthcare.
Carved Turn Control with Gate Vision Recognition of a Humanoid Robot for Giant Slalom Skiing on Ski Slopes
Authors: Park Cheonyu , Kim Baekseok , Kim Yitaek , Eum Younseal , Song Hyunjong , Yoon Dongkuk , Moon Jeongin , Han Jeakweon
Published: January 2022

The performance of humanoid robots is improving, owing in part to their participation in robot games such as the DARPA Robotics Challenge. Along with the 2018 Winter Olympics in Pyeongchang, a Skiing Robot Competition was held in which humanoid robots participated autonomously in a giant slalom alpine skiing competition. The robots were required to transit through many red or blue gates on the ski slope to reach the finish line. The course was relatively short at 100 m long and had an intermediate-level rating. A 1.23 m tall humanoid ski robot, ‘DIANA’, was developed for this skiing competition. As a humanoid robot that mimics humans, the goal was to descend the slope as fast as possible, so the robot was developed to perform a carved turn motion. The carved turn was difficult to balance compared to other turn methods. Therefore, ZMP control, which could secure the posture stability of the biped robot, was applied. Since skiing takes place outdoors, it was necessary to ensure recognition of the flags in various weather conditions. This was ensured using deep learning-based vision recognition. Thus, the performance of the humanoid robot DIANA was established using the carved turn in an experiment on an actual ski slope. The ultimate vision for humanoid robots is for them to naturally blend into human society and provide necessary services to people. Previously, there was no way for a full-sized humanoid robot to move on a snowy mountain. In this study, a humanoid robot that transcends this limitation was realized.
Auto-Exposure Algorithm for Enhanced Mobile Robot Localization in Challenging Light Conditions
Authors: Bégin Marc-André , Hunter Ian
Published: January 2022

The success of robot localization based on visual odometry (VO) largely depends on the quality of the acquired images. In challenging light conditions, specialized auto-exposure (AE) algorithms that purposely select camera exposure time and gain to maximize the image information can therefore greatly improve localization performance. In this work, an AE algorithm is introduced which, unlike existing algorithms, fully leverages the camera’s photometric response function to accurately predict the optimal exposure of future frames. It also features feedback that compensates for prediction inaccuracies due to image saturation and explicitly balances motion blur and image noise effects. For validation, stereo cameras mounted on a custom-built motion table allow different AE algorithms to be benchmarked on the same repeated reference trajectory using the stereo implementation of ORB-SLAM3. Experimental evidence shows that (1) the gradient information metric appropriately serves as a proxy of indirect/feature-based VO performance; (2) the proposed prediction model based on simulated exposure changes is more accurate than using γ transformations; and (3) the overall accuracy of the estimated trajectory achieved using the proposed algorithm equals or surpasses classic exposure control approaches. The source code of the algorithm and all datasets used in this work are shared openly with the robotics community.
Final Year Undergraduate Students’ Representation of the COVID-19 Pandemic and the Lockdown: Adaptability and Responsibility
Authors: Borcoman Mariana , Sorea Daniela
Published: January 2022

The COVID-19 pandemic has generated a new reality worldwide and reconfigured identities, behaviors and interests. It has called for heroic representations and highlighted the role of social media in efficient communication. All of the above considered, the current article focuses on the representation of the COVID-19 pandemic generated by the undergraduate students enrolled in the Social Work study program in Transilvania University of Brașov (Romania) by indicating the main connotations of the pandemic and drawing a comparison between students’ representation and the early representations of the same pandemic produced by specialized literature on the topic. The thematic analysis of the essays produced by students highlights their frustration with the havoc brought about by the pandemic to their graduation plans and with the lack of interaction with colleagues and academic staff, as well as their gratitude for the efforts undertaken by their professors to make online education functional, their availability to adapt and support the restrictive measures imposed by authorities, and their optimism about the evolution of the pandemic. The content analysis of 60 bibliographic sources on the topic of COVID-19, indicated by the Anelis+ network as the most relevant in the spring of 2020, highlights a thematic convergence between the aforementioned sources and students’ representation of COVID-19, and thus their responsible attitude toward meeting the challenges of the pandemic.
Social Capital and Loan Cost: The Role of Interpersonal Trust
Authors: Becchetti Leonardo , Manfredonia Stefano , Pisani Fabio
Published: January 2022

We argue that social dilemmas structured as investment trust games are a dominant feature in social and economic life due to asymmetric information, incomplete contracts and non-overlapping competencies that are typical characteristics of business relationships. We therefore consider that borrowers living in geographical areas with higher interpersonal trust are more likely to overcome the coordination failures typical of this kind of social dilemmas, thereby creating higher economic value and reducing the risk of their economic activity. Our empirical findings support this hypothesis, showing that lenders charge significantly lower loan costs on borrowers living in areas characterized by higher interpersonal trust.
Can Sustainable Development Save Mangroves?
Authors: Ferreira Alexander Cesar , Borges Rebecca , de Lacerda Luiz Drude
Published: January 2022

The Earth is warming, ecosystems are being overexploited, oceans are being polluted, and thousands of species are going extinct—all fueled by the need for a permanent increase in production for more consumerism and development. “Business as usual” continues untouched, while increasing attention has been given to the “sustainable development” concept. Despite their importance as life supporting ecosystems, forests, oceans, and wetlands are being destroyed at an accelerating rate. The conservation and restoration of mangroves, for example, are also vital for the planet to face catastrophic global warming. Based on a non-systematic literature review, we address how true mangrove conservation is incompatible with so-called “sustainable development”. We turn to the urgent changes needed to avoid environmental and societal collapse, promoted by the Western economic development paradigm, and address why the sustainable development approach has failed to stop environmental degradation and protect resources for next generations. Proposed solutions involve the rejection of the capital-oriented, nature-predatory systems, degrowth, a deep transformation of our energy matrix, and a shift in our nutrition to lower levels of the food chain. These are based on a profound sense of responsibility over the planet, respecting all life forms, ecosystem dynamics, and life sustaining properties of the biosphere.
Role of Physiology, Immunity, Microbiota, and Infectious Diseases in the Gut Health of Poultry
Authors: Wickramasuriya Samiru S. , Park Inkyung , Lee Kyungwoo , Lee Youngsub , Kim Woo H. , Nam Hyoyoun , Lillehoj Hyun S.
Published: January 2022

“Gut health” refers to the physical state and physiological function of the gastrointestinal tract and in the livestock system; this topic is often focused on the complex interacting components of the intestinal system that influence animal growth performance and host-microbial homeostasis. Regardless, there is an increasing need to better understand the complexity of the intestinal system and the various factors that influence gut health, since the intestine is the largest immune and neuroendocrine organ that interacts with the most complex microbiome population. As we face the post-antibiotic growth promoters (AGP) era in many countries of the world, livestock need more options to deal with food security, food safety, and antibiotic resilience to maintain agricultural sustainability to feed the increasing human population. Furthermore, developing novel antibiotic alternative strategies needs a comprehensive understanding of how this complex system maintains homeostasis as we face unpredictable changes in external factors like antibiotic-resistant microbes, farming practices, climate changes, and consumers’ preferences for food. In this review, we attempt to assemble and summarize all the relevant information on chicken gut health to provide deeper insights into various aspects of gut health. Due to the broad and complex nature of the concept of “gut health”, we have highlighted the most pertinent factors related to the field performance of broiler chickens.