bluuebunny/crossref_metadata_2025 huggingface dataset:
Deficits in force production during multifinger tasks demarcate cognitive dysfunction
Authors: Carson Richard G. , Berdondini Debora , Crosbie Maebh , McConville Caoilan , Forbes Shannon , Stewart Marla , Chiu Ruth Zhi Xian
Published: April 2024

Background

The multifinger force deficit (MFFD) is the decline in force generated by each finger as the number of fingers contributing to an action is increased. It has been shown to associate with cognitive status.

Aims

The aim was to establish whether a particularly challenging form of multifinger grip dynamometry, that provides minimal tactile feedback via cutaneous receptors and requires active compensation for reaction forces, will yield an MFFD that is more sensitive to cognitive status.

Methods

Associations between measures of motor function, and cognitive status (Montreal Cognitive Assessment [MoCA]) and latent components of cognitive function (derived from 11 tests using principal component analysis), were estimated cross-sectionally using generalized partial rank correlations. The participants (n =  62) were community dwelling, aged 65–87.

Results

Approximately half the participants were unable to complete the dynamometry task successfully. Cognitive status demarcated individuals who could perform the task from those who could not. Among those who complied with the task requirements, the MFFD was negatively correlated with MoCA scores—those with the highest MoCA scores tended to exhibit the smallest deficits, and vice versa. There were corresponding associations with latent components of cognitive function.

Discussion

The results support the view that neurodegenerative processes that are a feature of normal and pathological aging exert corresponding effects on expressions of motor coordination—in multifinger tasks, and cognitive sufficiency, due to their dependence on shared neural systems.

Conclusions

The outcomes add weight to the assertion that deficits in force production during multifinger tasks are sensitive to cognitive dysfunction.
Bianchi period polynomials: Hecke action and congruences
Authors: Combes Lewis
Published: April 2024

Let $$\Gamma ={{\,\mathrm{\textrm{PSL}}\,}}_2(\mathcal {O})$$

Γ
=

PSL

2

(
O
)

be a Bianchi group associated to one of the five Euclidean imaginary quadratic fields. We show that the space of weight k period polynomials for $$\Gamma $$
Γ
is “dual” to the space of weight k modular symbols for $$\Gamma $$
Γ
, reflecting the duality between the first and second cohomology groups of the arithmetic group $$\Gamma $$
Γ
. Using this result, we describe the action of Hecke operators on the space of period polynomials for $$\Gamma $$
Γ
via the Heilbronn matrices. As in the classical case, spaces of Bianchi period polynomials are related to parabolic cohomology of Bianchi groups, and in turn, via the Eichler–Shimura–Harder Isomorphism, to spaces of Bianchi modular forms. In the second part of the paper, we numerically investigate congruences between level 1 Bianchi eigenforms via computer programs which implement the above mentioned Hecke action on spaces of Bianchi period polynomials. Computations with the Hecke action are used to indicate moduli of congruences between the underlying Bianchi forms; we then prove the congruences using the period polynomials. From this we find congruences between genuine Bianchi modular forms and both a base-change Bianchi form and an Eisenstein series. We believe these congruences are the first of their kind in the literature.
Reliability and validity of the adapted Finnish version of the early onset scoliosis questionnaire (EOSQ-24)
Authors: Haapala Hermanni , Salonen Anne , Suominen Eetu , Syvänen Johanna , Repo Jussi , Matsumoto Hiroko , Ahonen Matti , Helenius Ilkka , Saarinen Antti
Published: April 2024

Background

EOSQ-24 is a disease specific patient-reported outcome score used to assess the quality of life in patients with early-onset scoliosis. The aim of this study was to translate and cross-culturally adapt the English version of the EOSQ-24 to Finnish language and to assess the reliability and validity of the translation.

Methods

Cross-cultural adaptation and cross-cultural validation were performed to the Finnish translation of the EOSQ-24. Patients and/or their caretakers were then recruited to assess the psychometric properties of the translation. We assessed the internal consistency, test–retest reliability, floor and ceiling effects, and discriminative abilities. One-hundred-and-three patients filled the questionnaire.

Results

EOSQ-24 was successfully translated into Finnish. The translation showed excellent internal consistency (Cronbach alpha 0.94), satisfactory item-total correlations ranging from 0.6 to 0.9, and moderate to strong inter item correlations. Test–retest reliability ranged from 0.7 to 0.96 indicating good to excellent agreement. Patients with neuromuscular and syndromic scoliosis reported lower EOSQ-24 scores when compared to patients’ idiopathic and congenital scoliosis. There was a significant negative correlation between major curve and EOSQ-24 scores in patients with idiopathic early onset scoliosis.

Conclusion

The internal consistency and test–retest reliability of the measure were found to be satisfactory. A marked ceiling effect was observed, indicating a potential source of error.
Biomechanical analysis of a trans-discal, multi-level stabilization screw (MLSS) at the upper instrumented vertebra (UIV) of long posterior thoracolumbar instrumentations
Authors: Collins Andrew P. , Shah Anoli A. , Shekouhi Niloufar , Goel Vijay K. , Theologis Alekos A.
Published: April 2024

Purpose

To evaluate proximal junctional biomechanics of a MLSS relative to traditional pedicle screw fixation at the proximal extent of T10-pelvis posterior instrumentation constructs (T10-p PSF).

Methods

A previously validated three-dimensional osseoligamentous spinopelvic finite element (FE) model was used to compare proximal junctional range-of-motion (ROM), vertebral body stresses, and discal biomechanics between two groups: (1) T10-p with a T10-11 MLSS (“T10-11 MLSS”) and (2) T10-p with a traditional T10 pedicle screw (“Traditional T10-PS”).

Results

The T10-11 MLSS had a 5% decrease in T9 cortical bone stress compared to Traditional T10-PS. Conversely, the T10 and T11 bone stresses increased by 46% and 98%, respectively, with T10-11 MLSS compared to Traditional T10-PS. Annular stresses and intradiscal pressures (IDP) were similar at T9-T10 between constructs. At the T10-11 disc, T10-11 MLSS decreased annular stresses by 29% and IDP by 48% compared to Traditional T10-PS. Adjacent ROM (T8-9 & T9-10) were similar between T10-11 MLSS and Traditional T10-PS. T10-11 MLSS had 39% greater ROM at T10-11 and 23% less ROM at T11-12 compared to Traditional T10-PS.

Conclusions

In this FE analysis, a T10-11 MLSS at the proximal extent of T10-pelvis posterior instrumentation resulted in increased T10 and T11 cortical bone stresses, decreased discal annular stress and IDP and increased ROM at T10-11, and no change in ROM at the adjacent level. Given the complex and multifactorial nature of proximal junctional kyphosis, these results require additional biomechanical and clinical evaluations to determine the clinical utility of MLSS on the proximal junctions of thoracolumbar posterior instrumented fusions.
The role of framing and effort in green nudging acceptance
Authors: Grelle Sonja , Kuhn Sascha , Fuhrmann-Riebel Hanna , Hofmann Wilhelm
Published: April 2024

Whether nudges succeed in promoting pro-environmental behavior strongly depends on their public acceptance. Prior literature shows that the framing of nudges, i.e., whether they address the individual (personal framing) or the society (societal framing), is one critical factor in determining nudging acceptance. Since a personal framing highlights the costs individuals have to bear to comply, we hypothesize that people accept nudges more when addressing the general public rather than themselves personally. We expect the framing effect to be stronger for nudges that elicit high-effort behavior than low-effort behavior. Results of multilevel linear regression analyses in two online experiments (nStudy 1 = 294, nobs = 4,410; nStudy 2 = 565, nobs = 11,300) reveal an opposite pattern: People accept nudges more when personally (vs societally) framed. As predicted, nudges receive higher support when the promoted behavior is perceived as low effort. Exploratory path analysis in Study 2 shows that the perceived effectiveness of the nudge mediates the positive relation between personal framing and nudging acceptance. This project provides novel insights on facilitators and barriers in nudging acceptance and their implications for policy-making.
Sightings of Bryde's whale, Balaenoptera edeni, off the Pacific coast of Nicaragua
Authors: De Weerdt Joëlle , Patulny Carla , Ruiz Pérez Nerea , Blanchet Leslie
Published: April 2024

Here, we report the first sighting records of Bryde's whales in Nicaragua. Four sightings were made in 2022 during boat-based surveys off the southwestern coast of Nicaragua. Photo-identification, distributional data, dive times, and behavioural information were collected, and environmental parameters, including sea surface temperature, were measured for each sighting. Sightings included calf and non-calf groups displaying travelling (n = 1; 25%), milling (n = 1; 25%), and suspected feeding (n = 2; 50%) behaviours. Approximately 4 h of focal following allowed the calculation of short and long breath intervals. Based on our observations, the individuals were suspected of feeding in the area, based on the presence of feeding birds, feeding humpback whales, relatively high time-lagged chlorophyll-a, and observed general behaviours. Our findings indicate that this species may occasionally visit Nicaraguan coastal areas in search of feeding opportunities, and could represent a possible range extension of the Eastern North Pacific stock.
Relevance of shock waves derived from asteroid impacts in the atmosphere of the early Earth in the production of compounds of astrobiological interest
Authors: Ramírez-Vázquez Luisa , Cordero-Tercero Guadalupe , de la Rosa José , Cruz-Castañeda Jorge Armando , Negrón-Mendoza Alicia
Published: April 2024

The generation of organic compounds relevant to the origin of living beings is easily achieved if reducing conditions exist in the environment; however, proposed models of primitive atmospheres do not favour these conditions. This work considers the quantity and possible size of the cosmic bodies that could have impacted the Earth between 4.2 and 3.8 Ga. Different atmospheres (with gases such as CO2, CO, N2, CH4) were experimentally irradiated by an Nd-YAG laser (used to simulate the energy of a shock wave produced by the interaction of a cosmic body with the atmosphere). Although the main products are short-chain, saturated and unsaturated hydrocarbons, hydrogen cyanide (HCN) is the most abundant in some atmospheres. HCN is an important precursor of the organic molecules relevant to chemical evolution. According to our calculations, between 1023 and 1025 g of HCN could have been produced by the energy released to the atmosphere from the entry of cosmic objects between 4.2 and 3.8 Ga. Therefore, this shock wave energy could play an important role in the processes of chemical evolution.
De-dehumanization: Practicing humanity
Authors: Deffenbaugh Natalie
Published: April 2024

The concept of humanity has been much discussed with respect to humanitarian work and international humanitarian law. There is today an idea of a single humanity, with each member equally valued beyond superficial differences in belief, nationality, ethnicity etc., and a global legal framework exists to prevent needless human suffering, including in war.

Dehumanization arises linguistically as the negation of a common, positive and mutually supportive humanity, though there is no single definition, and it certainly predates its opposite. Research indicates that dehumanization increases the risk of conflict/violence, increases the risk of abuses therein, and makes it harder to resolve conflict.

This paper gives an overview of how humanity is currently defined and used, notably by the International Committee of the Red Cross (ICRC) as one Fundamental Principle of the International Red Cross and Red Crescent Movement, and what dehumanization means especially in relation to conflict and violence. The paper then explores why and how dehumanization happens and the real-world harm that can result when it is espoused or tacitly condoned by those in positions of power. Finally, the paper examines how global legal frameworks and the principle of humanity, bolstered by impartiality, independence and neutrality, in particular as enacted by the ICRC, work to curb and push back against some of the worst harms that dehumanization can cause.
How Transformational Leadership Affects the Off-work Recovery of Daily Personal Energy Resources via Work Engagement: Resource and Demand-based Pathways
Authors: Czakert Jan Philipp , Leiva Ureña David , Berger Rita Gisela
Published: April 2024

This study focuses on the transformational leadership-work engagement relationship by investigating resource and demand pathways for daily off-work recovery and employee wellbeing (EWB). While previous research highlighted how transformational leadership energizes employees to engage at work, energy is a finite resource requiring daily restoration for EWB. Yet, how the leader’s energizing effect relates to daily employees’ recovery remains unknown. Following job demands-resource-recovery theory, we test two pathways that relate the transformational leadership-work engagement relationship to daily employee recovery: (a) Resource-based via resource-building, (b) demand-based via increased demands. Utilizing a 10-day, two daily measurement (N = 88) study, multilevel path analyses revealed: transformational leadership predicted via work engagement (b = .17, p < .05) role clarity (b = .56, p < .01), then positive (b = .39, p < .01), and negative work-nonwork spillover (b = –.38, p < .01). Positive work-nonwork spillover predicted recovery positively (b = .25, p < .01), negative work-nonwork spillover negatively (b = –.40, p < .01). Recovery predicted EWB for positive (b = .38, p < .01) and for negative (b = –.43, p < .01) affect. Work engagement predicted workload (b = .35, p < .01), further negative (b = .33, p < .01) and positive work-nonwork spillover (b = –.16, p < .01), hampering EWB. As one pathway effect might cancel the other, the main effect of transformational leadership on EWB was not significant in the integrative model (p > .05). Results highlight dark and bright sides of the transformational leadership-work engagement relationship regarding daily recovery.
Mega‐Folding of a Basement During Incipient Intra‐Plate Continental Subduction (Alpine Central Iberia)
Authors: Díez Fernández Rubén , de Vicente Gerardo , Moreno‐Martín Diana , Fernández Carlos , Arenas Ricardo , Rubio Pascual Francisco J.
Published: April 2024

Metamorphic basements are usually considered rigid and isotropic at a large scale. However, basements contain inherited weaknesses that may potentially accommodate superimposed contraction (e.g., fault reactivation), and that favor fold nucleation (e.g., penetrative foliations). If these conditions are met, what could be the factors that impede the development of basement folds or their recognition? Actual basement folding is rarely documented, especially for large dimensions. Here we provide a case example, discussed from the perspective of structural analysis of surface data and sustained by geophysical data. The basement of the Spanish‐Portuguese Central System is defined by an Alpine mega‐fold (Hiendelaencina Antiform) that trends parallel to this mountain range and affects the basement and its sedimentary cover, collectively. The wavelength of this fold matches or even surpasses the thickness of the crust that hosts it (36–41 km). The Moho under this mega‐fold is displaced by an Alpine fault that accounts for incipient intraplate continental subduction. The topography of the mantle may reflect an up‐warping compatible with the mega‐fold observed on the surface. Mega‐folding is observed in the hanging wall of the Berzosa Fault, which emerges as a SE‐dipping, Variscan (Paleozoic), extensional fault reactivated as a basal decollement upon Alpine (Cenozoic) contraction. The mega‐fold was formed after well‐oriented planar anisotropies in the basement (foliation and bedding). The development of this fold was assisted by heterogeneous shearing (coeval thrusting) plus the buttressing effect of pre‐existing, near‐vertical, crustal‐scale faults (Somolinos and Somosierra), which inhibited slip‐upsection through the basal decollement (Berzosa Fault).
Genetic influences and causal pathways shared between cannabis use disorder and other substance use traits
Authors: Galimberti Marco , Levey Daniel F. , Deak Joseph D. , Zhou Hang , Stein Murray B. , Gelernter Joel
Published: April 2024

Cannabis use disorder (CanUD) has increased with the legalization of the use of cannabis. Around 20% of individuals using cannabis develop CanUD, and the number of users has grown with increasing ease of access. CanUD and other substance use disorders (SUDs) are associated phenotypically and genetically. We leveraged new CanUD genomics data to undertake genetically-informed analyses with unprecedented power, to investigate the genetic architecture and causal relationships between CanUD and lifetime cannabis use with risk for developing SUDs and substance use traits. Analyses included calculating local and global genetic correlations, genomic structural equation modeling (genomicSEM), and Mendelian Randomization (MR). Results from the genetic correlation and genomicSEM analyses demonstrated that CanUD and cannabis use differ in their relationships with SUDs and substance use traits. We found significant causal effects of CanUD influencing all the analyzed traits: opioid use disorder (OUD) (Inverse variant weighted, IVW β = 0.925 ± 0.082), problematic alcohol use (PAU) (IVW β = 0.443 ± 0.030), drinks per week (DPW) (IVW β = 0.182 ± 0.025), Fagerström Test for Nicotine Dependence (FTND) (IVW β = 0.183 ± 0.052), cigarettes per day (IVW β = 0.150 ± 0.045), current versus former smokers (IVW β = 0.178 ± 0.052), and smoking initiation (IVW β = 0.405 ± 0.042). We also found evidence of bidirectionality showing that OUD, PAU, smoking initiation, smoking cessation, and DPW all increase risk of developing CanUD. For cannabis use, bidirectional relationships were inferred with PAU, smoking initiation, and DPW; cannabis use was also associated with a higher risk of developing OUD (IVW β = 0.785 ± 0.266). GenomicSEM confirmed that CanUD and cannabis use load onto different genetic factors. We conclude that CanUD and cannabis use can increase the risk of developing other SUDs. This has substantial public health implications; the move towards legalization of cannabis use may be expected to increase other kinds of problematic substance use. These harmful outcomes are in addition to the medical harms associated directly with CanUD.
Low Functional network integrity in cognitively unimpaired and MCI subjects with depressive symptoms: results from a multi-center fMRI study
Authors: Csukly Gabor , Tombor László , Hidasi Zoltan , Csibri Eva , Fullajtár Máté , Huszár Zsolt , Koszovácz Vanda , Lányi Orsolya , Vass Edit , Koleszár Boróka , Kóbor István , Farkas Katalin , Rosenfeld Viktoria , Berente Dalida Borbála , Bolla Gergo , Kiss Mate , Kamondi Anita , Horvath Andras Attila
Published: April 2024

Evidence suggests that depressive symptomatology is a consequence of network dysfunction rather than lesion pathology. We studied whole-brain functional connectivity using a Minimum Spanning Tree as a graph-theoretical approach. Furthermore, we examined functional connectivity in the Default Mode Network, the Frontolimbic Network (FLN), the Salience Network, and the Cognitive Control Network. All 183 elderly subjects underwent a comprehensive neuropsychological evaluation and a 3 Tesla brain MRI scan. To assess the potential presence of depressive symptoms, the 13-item version of the Beck Depression Inventory (BDI) or the Geriatric Depression Scale (GDS) was utilized. Participants were assigned into three groups based on their cognitive status: amnestic mild cognitive impairment (MCI), non-amnestic MCI, and healthy controls. Regarding affective symptoms, subjects were categorized into depressed and non-depressed groups. An increased mean eccentricity and network diameter were found in patients with depressive symptoms relative to non-depressed ones, and both measures showed correlations with depressive symptom severity. In patients with depressive symptoms, a functional hypoconnectivity was detected between the Anterior Cingulate Cortex (ACC) and the right amygdala in the FLN, which impairment correlated with depressive symptom severity. While no structural difference was found in subjects with depressive symptoms, the volume of the hippocampus and the thickness of the precuneus and the entorhinal cortex were decreased in subjects with MCI, especially in amnestic MCI. The increase in eccentricity and diameter indicates a more path-like functional network configuration that may lead to an impaired functional integration in depression, a possible cause of depressive symptomatology in the elderly.
Giant gate modulation of antiferromagnetic spin reversal by the magnetoelectric effect
Authors: Ujimoto Kakeru , Sameshima Hiroki , Toyoki Kentaro , Moriyama Takahiro , Nakamura Kohji , Kotani Yoshinori , Suzuki Motohiro , Iino Ion , Kawamura Naomi , Nakatani Ryoichi , Shiratsuchi Yu
Published: April 2024

In this study, using the Pt/Cr2O3/Pt epitaxial trilayer, we demonstrate the giant voltage modulation of the antiferromagnetic spin reversal and the voltage-induced 180° switching of the Néel vector in maintaining a permanent magnetic field. We obtained a significant modulation efficiency of the switching field, Δμ
0
H
SW/ΔV (Δμ
0
H
SW/ΔE), reaching a maximum of −500 mT/V (−4.80 T nm/V); this value was more than 50 times greater than that of the ferromagnetic-based counterparts. From the temperature dependence of the modulation efficiency, X-ray magnetic circular dichroism measurements and first-principles calculations, we showed that the origin of the giant modulation efficiency relied on the electric field modulation of the net magnetization due to the magnetoelectric effect. From the first-principles calculation and the thickness effect on the offset electric field, we found that the interfacial magnetoelectric effect emerged. Our demonstration reveals the energy-efficient and widely applicable operation of an antiferromagnetic spin based on a mechanism distinct from magnetic anisotropy control.
Further clarification of cognitive processes of prospective memory in schizophrenia by comparing eye-tracking and ecologically-valid measurements
Authors: Li Hang , Wang Qi , Hou Wen-Peng , Chen Dong-Yang , Ding Yu-Shen , Zhang Zhi-Fang , Hou Wei-Wei , Sha Sha , Yang Ning-Bo , Bo Qi-Jing , Wang Ya , Zhou Fu-Chun , Wang Chuan-Yue
Published: April 2024

The aim of this study is to compare ecologically-valid measure (the Cambridge Prospective Memory Test, CAMPROMPT) and laboratory measure (eye-tracking paradigm) in assessing prospective memory (PM) in individuals with schizophrenia spectrum disorders (SSDs). In addition, eye-tracking indices are used to examine the relationship between PM and other cognitive domains in SSDs patients. Initially, the study sample was formed by 32 SSDs patients and 32 healthy control subjects (HCs) who were matched in sociodemographic profile and the performance on CAMPROMPT. An eye-tracking paradigm was employed to examine the differences in PM accuracy and key cognitive processes (e.g., cue monitoring) between the two groups. Additional 31 patients were then recruited to investigate the relationship between PM cue monitoring, other cognitive functions, and the severity of clinical symptoms within the SSDs group. The monitoring of PM cue was reflected in total fixation time and total fixation counts for distractor words. Cognitive functions were assessed using the Chinese version of the MATRICS Consensus Cognitive Battery (MCCB). The Positive and Negative Syndrome Scale (PANSS) was applied to assess psychopathology. SSDs patients exhibited fewer total fixation counts for distractor words and lower PM accuracy compared to HCs, even though they were priori matched on CAMPROMPT. Correlation analysis within the SSDs group (63 cases) indicated a negative correlation between PM accuracy and PANSS total score, and a positive correlation with working memory and attention/vigilance. Regression analysis within the SSDs group revealed that higher visual learning and lower PANSS total scores independently predicted more total fixation counts on distractor words. Impairment in cue monitoring is a critical factor in the PM deficits in SSDs. The eye-tracking laboratory paradigm has advantages over the ecologically-valid measurement in identifying the failure of cue detection, making it a more sensitive tool for PM deficits in patients with SSDs.
Chromosome-level genome assembly of milk thistle (Silybum marianum (L.) Gaertn.)
Authors: Kim Kyung Do , Shim Jeehyoung , Hwang Ji-Hun , Kim Daegwan , El Baidouri Moaine , Park Soyeon , Song Jiyong , Yu Yeisoo , Lee Keunpyo , Ahn Byoung-Ohg , Hong Su Young , Chin Joong Hyoun
Published: April 2024

Silybum marianum (L.) Gaertn., commonly known as milk thistle, is a medicinal plant belonging to the Asteraceae family. This plant has been recognized for its medicinal properties for over 2,000 years. However, the genome of this plant remains largely undiscovered, having no reference genome at a chromosomal level. Here, we assembled the chromosome-level genome of S. marianum, allowing for the annotation of 53,552 genes and the identification of transposable elements comprising 58% of the genome. The genome assembly from this study showed 99.1% completeness as determined by BUSCO assessment, while the previous assembly (ASM154182v1) showed 36.7%. Functional annotation of the predicted genes showed 50,329 genes (94% of total genes) with known protein functions in public databases. Comparative genome analysis among Asteraceae plants revealed a striking conservation of collinearity between S. marianum and C. cardunculus. The genomic information generated from this study will be a valuable resource for milk thistle breeding and for use by the larger research community.
Dataset of building locations in Poland in the 1970s and 1980s
Authors: Szubert Piotr , Kaim Dominik , Kozak Jacek
Published: April 2024

The aim of this study was to create a dataset of building locations in Poland from the 1970s–1980s. The source information was the historical 1:10 000 Polish topographic map. Building footprints were detected and extracted from approximately 8,500 scanned map sheets using the Mask R-CNN model implemented in Esri ArcGIS Pro software, and converted to point building locations. The dataset of building locations covers the entire country and contains approximately 11 million points representing buildings. The accuracy of the dataset was assessed manually on randomly selected map sheets. The overall accuracy is 95% (F1 = 0.98). The dataset may be used in conjunction with various contemporary land use, land cover and cadastral datasets in a broad range of applications related to long-term changes in rural and urban areas, including urban sprawl and its environmental and social consequences. It can also serve as a highly reliable reference dataset for regional or global settlement products derived, e.g., from early Landsat data.
A chromosome-scale fishing cat reference genome for the evaluation of potential germline risk variants
Authors: Carroll Rachel A. , Rice Edward S. , Murphy William J. , Lyons Leslie A. , Thibaud-Nissen Francoise , Coghill Lyndon M. , Swanson William F. , Terio Karen A. , Boyd Tyler , Warren Wesley C.
Published: April 2024

The fishing cat, Prionailurus viverrinus, faces a population decline, increasing the importance of maintaining healthy zoo populations. Unfortunately, zoo-managed individuals currently face a high prevalence of transitional cell carcinoma (TCC), a form of bladder cancer. To investigate the genetics of inherited diseases among captive fishing cats, we present a chromosome-scale assembly, generate the pedigree of the zoo-managed population, reaffirm the close genetic relationship with the Asian leopard cat (Prionailurus bengalensis), and identify 7.4 million single nucleotide variants (SNVs) and 23,432 structural variants (SVs) from whole genome sequencing (WGS) data of healthy and TCC cats. Only BRCA2 was found to have a high recurrent number of missense mutations in fishing cats diagnosed with TCC when compared to inherited human cancer risk variants. These new fishing cat genomic resources will aid conservation efforts to improve their genetic fitness and enhance the comparative study of feline genomes.
Delineation of three-dimensional tumor margins based on normalized absolute difference mapping via volumetric optical coherence tomography
Authors: Park Jae-Sung , Yoon Taeil , Park Soon A. , Lee Byeong Ha , Jeun Sin-Soo , Eom Tae Joong
Published: April 2024

The extent of surgical resection is an important prognostic factor in the treatment of patients with glioblastoma. Optical coherence tomography (OCT) imaging is one of the adjunctive methods available to achieve the maximal surgical resection. In this study, the tumor margins were visualized with the OCT image obtained from a murine glioma model. A commercialized human glioblastoma cell line (U-87) was employed to develop the orthotopic murine glioma model. A swept-source OCT (SS-OCT) system of 1300 nm was used for three-dimensional imaging. Based on the OCT intensity signal, which was obtained via accumulation of each A-scan data, an en-face optical attenuation coefficient (OAC) map was drawn. Due to the limited working distance of the focused beam, OAC values decrease with depth, and using the OAC difference in the superficial area was chosen to outline the tumor boundary, presenting a challenge in analyzing the tumor margin along the depth direction. To overcome this and enable three-dimensional tumor margin detection, we converted the en-face OAC map into an en-face difference map with x- and y-directions and computed the normalized absolute difference (NAD) at each depth to construct a volumetric NAD map, which was compared with the corresponding H&E-stained image. The proposed method successfully revealed the tumor margin along the peripheral boundaries as well as the margin depth. We believe this method can serve as a useful adjunct in glioma surgery, with further studies necessary for real-world practical applications.
Social inattentional blindness to idea stealing in meetings
Authors: Masters-Waage Theodore C. , Kinias Zoe , Argueta-Rivera Jazmin , Stewart Dillon , Ivany Rachel , King Eden , Hebl Mikki
Published: April 2024

Using a virtual reality social experiment, participants (N = 154) experienced being at the table during a decision-making meeting and identified the best solutions generated. During the meeting, one meeting participant repeated another participant’s idea, presenting it as his own. Although this idea stealing was clearly visible and audible, only 30% of participants correctly identified who shared the idea first. Subsequent analyses suggest that the social environment affected this novel form of inattentional blindness. Although there was no experimental effect of team diversity on noticing, there was correlational evidence of an indirect effect of perceived team status on noticing via attentional engagement. In sum, this paper extends the inattentional blindness phenomenon to a realistic professional interaction and demonstrates how features of the social environment can reduce social inattention.
Population-based cohort study to investigate the changes in prevalence, severity profile, and treatment modalities used in Korean atopic dermatitis patients
Authors: Lee Hyun Ji , Oh Hyun Ju , Lee Gyu Na , Han Kyung Do , Lee Ji Hyun , Park Young Min
Published: April 2024

In this retrospective study spanning from 2002 to 2019, we analyzed data from 355,277 Korean patients diagnosed with atopic dermatitis (AD) through the National Health Insurance System. Our objective was to comprehensively analyze the trends in prevalence, severity profiles, and treatment approaches for AD in Korea over this 18-year period. Initially, AD prevalence stood at 3.88% in 2002 but notably rose to 5.03% by 2019. During the same period, while AD prevalence decreased in the 0–1-year-old group (from 34.52% to 24.83%), it remained relatively stable in the 1–11-year-old group. Conversely, the 12–19-year-old and 20 years or older age groups witnessed substantial increases in AD prevalence, climbing from 2.55 to 6.02% and 1.44% to 3.53%, respectively. Moreover, the proportion of patients classified as having moderate to severe AD grew from 30.96 to 39.78%. Surprisingly, the prescription pattern, predominantly based on corticosteroid administration, exhibited minimal change despite the rising prevalence of moderate and severe AD cases. These findings underline a persistent reliance on corticosteroid-based treatments for AD, even as the condition's severity escalates among Korean adolescents and adults. Consequently, there is a pressing need to develop novel treatment guidelines emphasizing biologics that offer enhanced safety and efficacy.
Improving high throughput manufacture of laser-inscribed graphene electrodes via hierarchical clustering
Authors: Qian Hanyu , Moreira Geisianny , Vanegas Diana , Tang Yifan , Pola Cicero , Gomes Carmen , McLamore Eric , Bliznyuk Nikolay
Published: April 2024

Laser-inscribed graphene (LIG), initially developed for graphene supercapacitors, has found widespread use in sensor research and development, particularly as a platform for low-cost electrochemical sensing. However, batch-to-batch variation in LIG fabrication introduces uncertainty that cannot be adequately tracked during manufacturing process, limiting scalability. Therefore, there is an urgent need for robust quality control (QC) methodologies to identify and select similar and functional LIG electrodes for sensor fabrication. For the first time, we have developed a statistical workflow and an open-source hierarchical clustering tool for QC analysis in LIG electrode fabrication. The QC process was challenged with multi-operator cyclic voltammetry (CV) data for bare and metalized LIG. As a proof of concept, we employed the developed QC process for laboratory-scale manufacturing of LIG-based biosensors. The study demonstrates that our QC process can rapidly identify similar LIG electrodes from large batches (n ≥ 36) of electrodes, leading to a reduction in biosensor measurement variation by approximately 13% compared to the control group without QC. The statistical workflow and open-source code presented here provide a versatile toolkit for clustering analysis, opening a pathway toward scalable manufacturing of LIG electrodes in sensing. In addition, we establish a data repository for further study of LIG variation.
Predicting adverse events after thoracic endovascular aortic repair for patients with type B aortic dissection
Authors: Kang Mengyang , Li You , Zhang Yiman , Zhao Yang , Meng Yan , Zhang Junbo , Tian Hongyan
Published: April 2024

The potential of adverse events (AEs) after thoracic endovascular aortic repair (TEVAR) in patients with type B aortic dissection (TBAD) has been reported. To avoid the occurrence of AEs, it is important to recognize high-risk population for prevention in advance. The data of 261 patients with TBAD who received TEVAR between June 2017 and June 2021 at our medical center were retrospectively reviewed. After the implementation of exclusion criteria, 172 patients were finally included, and after 2.8 years (range from 1 day to 5.8 years) of follow up, they were divided into AEs (n = 41) and non-AEs (n = 131) groups. We identified the predictors of AEs, and a prediction model was constructed to calculate the specific risk of postoperative AEs at 1, 2, and 3 years, and to stratify patients into high-risk (n = 78) and low-risk (n = 94) group. The prediction model included seven predictors: Age > 75 years, Lower extremity malperfusion (LEM), NT-proBNP > 330 pg/ml, None distal tear, the ratio between the diameter of the ascending aorta and descending aorta (A/D ratio) > 1.2, the ratio of the area of the false lumen to the total aorta (FL ratio) > 64%, and acute TEVAR, which exhibited excellent predictive accuracy performance and discriminatory ability with C statistic of 82.3% (95% CI 77.3–89.2%). The prediction model was contributed to identify high-risk patients of postoperative AEs, which may serve to achievement of personalized treatment and follow-up plans for patients.
Depletion of proteasome subunit PSMD1 induces cancer cell death via protein ubiquitination and DNA damage, irrespective of p53 status
Authors: Kim Mi-Yeun , Park Eun-Ran , Cho Eung-Ho , Park Sun-Hoo , Han Chul Ju , Kim Sang-Bum , Gu Man Bock , Shin Hyun-Jin , Lee Kee-Ho
Published: April 2024

Hepatocellular carcinoma (HCC) is characterized by high incidence and fatality rates worldwide. In our exploration of prognostic factors in HCC, the 26s proteasome subunit, non-ATPase 1 (PSMD1) protein emerged as a significant contributor, demonstrating its potential as a therapeutic target in this aggressive cancer. PSMD1 is a subunit of the 19S regulatory particle in the 26S proteasome complex; the 19S particle controls the deubiquitination of ubiquitinated proteins, which are then degraded by the proteolytic activity of the complex. Proteasome-targeting in cancer therapy has received significant attention because of its practical application as an established anticancer agent. We investigated whether PSMD1 plays a critical role in cancer owing to its prognostic significance. PSMD1 depletion induced cell cycle arrest in G2/M phase, DNA damage and apoptosis of cancer cells, irrespective of the p53 status. PSMD1 depletion-mediated cell death was accompanied by an increase in overall protein ubiquitination. These phenotypes occurred exclusively in cancer cells, with no effects observed in normal cells. These findings indicate that PSMD1 depletion-mediated ubiquitination of cellular proteins induces cell cycle arrest and eventual death in cancer cells, emphasizing PSMD1 as a potential therapeutic target in HCC.
Automated imaging coupled with AI-powered analysis accelerates the assessment of plant resistance to Tetranychus urticae
Authors: Złotkowska Ewelina , Wlazło Anna , Kiełkiewicz Małgorzata , Misztal Krzysztof , Dziosa Paulina , Soja Krzysztof , Barczak-Brzyżek Anna , Filipecki Marcin
Published: April 2024

The two-spotted spider mite (TSSM), Tetranychus urticae, is among the most destructive piercing-sucking herbivores, infesting more than 1100 plant species, including numerous greenhouse and open-field crops of significant economic importance. Its prolific fecundity and short life cycle contribute to the development of resistance to pesticides. However, effective resistance loci in plants are still unknown. To advance research on plant-mite interactions and identify genes contributing to plant immunity against TSSM, efficient methods are required to screen large, genetically diverse populations. In this study, we propose an analytical pipeline utilizing high-resolution imaging of infested leaves and an artificial intelligence-based computer program, MITESPOTTER, for the precise analysis of plant susceptibility. Our system accurately identifies and quantifies eggs, feces and damaged areas on leaves without expert intervention. Evaluation of 14 TSSM-infested Arabidopsis thaliana ecotypes originating from diverse global locations revealed significant variations in symptom quantity and distribution across leaf surfaces. This analytical pipeline can be adapted to various pest and host species, facilitating diverse experiments with large specimen numbers, including screening mutagenized plant populations or phenotyping polymorphic plant populations for genetic association studies. We anticipate that such methods will expedite the identification of loci crucial for breeding TSSM-resistant plants.
Fingerprinting Mediterranean hurricanes using pre-event thermal drops in seawater temperature
Authors: Scardino Giovanni , Miglietta Mario Marcello , Kushabaha Alok , Casella Elisa , Rovere Alessio , Besio Giovanni , Borzì Alfio Marco , Cannata Andrea , Mazza Gianfranco , Sabato Gaetano , Scicchitano Giovanni
Published: April 2024

Extreme atmospheric-marine events, known as medicanes (short for “Mediterranean hurricanes”), have affected the Mediterranean basin in recent years, resulting in extensive coastal flooding and storm surges, and have occasionally been responsible for several casualties. Considering that the development mechanism of these events is similar to tropical cyclones, it is plausible that these phenomena are strongly affected by sea surface temperatures (SSTs) during their development period (winter and autumn seasons). In this study, we compared satellite data and the numerical reanalysis of SSTs from 1969 to 2023 with in situ data from dataloggers installed at different depths off the coast of southeastern Sicily as well as from data available on Argo floats on the Mediterranean basin. A spectral analysis was performed using a continuous wavelet transform (CWT) for each SST time series to highlight the changes in SSTs prior to the occurrence of Mediterranean Hurricanes as well as the energy content of the various frequencies of the SST signal. The results revealed that decreases in SST occurred prior to the formation of each Mediterranean hurricane, and that this thermal drop phenomenon was not observed in intense extra-tropical systems. The spectral analyses revealed that high CWT coefficients representing high SST energy contents were observed before the occurrence of a Mediterranean hurricane. This information may provide a useful fingerprint for distinguishing Mediterranean hurricanes from common seasonal storms at the onset of these events.
Artificial light at night bans Chaoborus from vital epilimnetic waters
Authors: Ślusarczyk Mirosław , Bednarska Anna , Zebrowski Marcin Lukasz , Tałanda Joanna
Published: April 2024

Artificial light at night (ALAN) is known to affect organisms in terrestrial ecosystems and adjacent litoral habitats. In the present study, we tested the effect of ALAN on the spatial distribution of organisms in open waters, using the insect larvae ofChaoborus flavicansas an example. During the dayC. flavicanstypically hide from visually hunting fish in deep, dark, anoxic waters. On safer nights, they forage in rich subsurface waters. Nighttime field tests revealed that light from an HPS street lamp mounted on a boat anchored in open water attracted planktivorous fish, but deterred planktonicChaoborusfrom rich but risky surface waters.Chaoborusdid not descend to the safest, anoxic hypolimnion, but remained in hypoxic mid-depth metalimnion, which does not appear to be a perfect refuge. Neither light gradient nor food distribution fully explained their mid-depth residence under ALAN conditions. A further laboratory test revealed a limited tolerance ofC. flavicansto anoxia. Half of the test larvae died after 38 h at 9 °C in anoxic conditions. The trade-off between predation risk and oxygen demand may explain whyChaoborusdid not hide in deep anoxic waters, but remained in the riskier metalimnion with residual oxygen under ALAN conditions.
Asthma prevalence among United States population insights from NHANES data analysis
Authors: Swed Sarya , Sawaf Bisher , Al-Obeidat Feras , Hafez Wael , Rakab Amine , Alibrahim Hidar , Nasif Mohamad Nour , Alghalyini Baraa , Zia Zaidi Abdul Rehman , Alshareef Lamees , Alqatati Fadel , Zamrath Zahir Fathima , Ahmed Ashraf I. , Alom Mulham , Sultan Anas , AlMahmoud Abdullah , Bakkour Agyad , Cherrez-Ojeda Ivan
Published: April 2024

Asthma is a prevalent respiratory condition that poses a substantial burden on public health in the United States. Understanding its prevalence and associated risk factors is vital for informed policymaking and public health interventions. This study aims to examine asthma prevalence and identify major risk factors in the U.S. population. Our study utilized NHANES data between 1999 and 2020 to investigate asthma prevalence and associated risk factors within the U.S. population. We analyzed a dataset of 64,222 participants, excluding those under 20 years old. We performed binary regression analysis to examine the relationship of demographic and health related covariates with the prevalence of asthma. The study found that asthma affected 8.7% of the U.S. population. Gender emerged as a significant factor, with 36.0% of asthma patients being male and 64.0% female (p < 0.001). Individuals aged 60 and older having the highest asthma prevalence at 34.0%. Non-Hispanic whites had the highest prevalence at 46.4%, followed by non-hispanic blacks at 26.0%. In contrast, Mexican Americans and other hispanic individuals had lower rates, at 9.6% and 9.0%, respectively. Females were 1.76 times more likely to have asthma than males (p < 0.001). Obese individuals had a 1.74 times higher likelihood of current asthma compared to underweight individuals (p < 0.001). Notably, both Non-Hispanic Whites and Non-Hispanic Blacks showed higher odds of current asthma compared to Mexican Americans (with adjusted odds ratios of 2.084 and 2.096, respectively, p < 0.001). The research findings indicate that asthma is prevalent in 8.7% of the U.S. population. Our study highlights that individuals who are female, have low income, are obese, and smoke have the highest likelihood of being affected by asthma. Therefore, public health policies should prioritize addressing these risk factors in their preventive strategies.
Refining outcome prediction after traumatic brain injury with machine learning algorithms
Authors: Bark D. , Boman M. , Depreitere B. , Wright D. W. , Lewén A. , Enblad P. , Hånell A. , Rostami E.
Published: April 2024

Outcome after traumatic brain injury (TBI) is typically assessed using the Glasgow outcome scale extended (GOSE) with levels from 1 (death) to 8 (upper good recovery). Outcome prediction has classically been dichotomized into either dead/alive or favorable/unfavorable outcome. Binary outcome prediction models limit the possibility of detecting subtle yet significant improvements. We set out to explore different machine learning methods with the purpose of mapping their predictions to the full 8 grade scale GOSE following TBI. The models were set up using the variables: age, GCS-motor score, pupillary reaction, and Marshall CT score. For model setup and internal validation, a total of 866 patients could be included. For external validation, a cohort of 369 patients were included from Leuven, Belgium, and a cohort of 573 patients from the US multi-center ProTECT III study. Our findings indicate that proportional odds logistic regression (POLR), random forest regression, and a neural network model achieved accuracy values of 0.3–0.35 when applied to internal data, compared to the random baseline which is 0.125 for eight categories. The models demonstrated satisfactory performance during external validation in the data from Leuven, however, their performance were not satisfactory when applied to the ProTECT III dataset.