bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Serial Remote Ischemic Conditioning on Dynamic Cerebral Autoregulation and Brain Injury Related Biomarkers
Authors: Qu Yang , Zhang Peng , He Qian-Yan , Sun Ying-Ying , Wang Mei-Qi , Liu Jia , Zhang Pan-Deng , Yang Yi , Guo Zhen-Ni
Published: February 2022

Objective

Recent studies have demonstrated the positive roles of remote ischemic conditioning (RIC) in patients with cerebrovascular diseases; however, the mechanisms remain unclear. This study aimed to explore the effect of serial RIC on dynamic cerebral autoregulation (dCA) and serum biomarkers associated with brain injury, both of which are related to the prognosis of cerebrovascular disease.

Methods

This was a self-controlled interventional study in healthy adults. The RIC was conducted twice a day for 7 consecutive days (d1–d7) and comprised 4 × 5-min single arm cuff inflation/deflation cycles at 200 mmHg. All participants underwent assessments of dCA ten times, including baseline, d1, d2, d4, d7, d8, d10, d14, d21, and d35 of the study. Blood samples were collected four times (baseline, d1, d7, and d8) immediately after dCA measurements. The transfer function parameters [phase difference (PD) and gain] were used to quantify dCA. Four serum biomarkers associated with brain injury, ubiquitin C-terminal hydrolase-L1, neuron-specific enolase, glial fibrillary acidic protein, and S100β were tested.

Results

Twenty-two healthy adult volunteers (mean age 25.73 ± 1.78 years, 3 men [13.6%], all Asian) were enrolled in this study. Bilateral PD values were significantly higher since four times of RIC were completed (d2) compared with PD values at baseline (left: 53.31 ± 10.53 vs. 45.87 ± 13.02 degree, p = 0.015; right: 54.90 ± 10.46 vs. 45.96 ± 10.77 degree, p = 0.005). After completing 7 days of RIC, the significant increase in dCA was sustained for at least 28 days (d35, left: 53.11 ± 14.51 degree, P = 0.038; right: 56.95 ± 14.57 degree, p < 0.001). No difference was found in terms of different serum biomarkers related to brain injury before and after RIC.

Conclusion

The elevation in dCA was detected immediately after four repeated times of RIC, and 7-day consecutive RIC induced a sustained increase in dCA for at least 28 days and did not affect blood biomarkers of brain injury in healthy adults. These results will help us to formulate detailed strategies for the safe and effective application of RIC in patients with cerebrovascular disease.
Are the Follicular Fluid Characteristics of Recovered Coronavirus Disease 2019 Patients Different From Those of Vaccinated Women Approaching in vitro Fertilization?
Authors: Castiglione Morelli Maria A. , Iuliano Assunta , Schettini Sergio C. A. , Ferri Angela , Colucci Paola , Viggiani Licia , Matera Ilenia , Ostuni Angela
Published: February 2022

The aim of this pilot study is to evaluate if SARS-CoV-2 infection or vaccination against SARS-CoV-2 infection induce observable metabolic effects in follicular fluid of women who are following in vitro fertilization (IVF) treatments. The possible impact of coronavirus disease 2019 (COVID-19) on fertility and IVF outcome is considered. We have selected for this study: six women vaccinated against SARS-CoV-2 infection, five recovered COVID-19 patients, and we used nine healthy women as the control group. At the time of oocytes retrieval from participants in the study, follicular fluids were collected and metabolomic analysis was performed by 1H NMR spectroscopy in combination with multivariate analysis to interpret the spectral data. The search for antibody positivity in the follicular fluid aspirates was also carried out, together with the western blotting analysis of some inflammatory proteins, interleukin-6, tumor necrosis factor α (TNFα), and the free radical scavenger superoxide dismutase 2. Higher levels of Ala and Pro together with lower levels of lipids and trimethylamine N-oxide (TMAO) were found in follicular fluids (FFs) of vaccinated women while lower levels of many metabolites were detected in FFs of recovered COVID patients. Expression level of TNF-α was significantly lower both in recovered COVID-19 patients and vaccinated women in comparison to healthy controls.
The Structure of Mental Elasticity Education for Children in Plight Using Deep Learning
Authors: Sun Xuanlu , Yang Xiaoyang
Published: February 2022

The purpose is to solve the problem that the current research on the impact of the microstructure of mental elasticity and its constituent factors on the development of the mental elasticity of children is not comprehensive, and the traditional artificial analysis method of mental problems has strong subjectivity and low accuracy. First, the structural equation model is used to study the microstructure of poor children's mental elasticity, and to explore the structural relationship and functional path between the mental elasticity of children and the self-efficacy of their mental health, psychological anxiety, and attachment. Second, a prediction model of mental problems of children in plight based on the backpropagation neural network (BPNN) is constructed. Finally, middle schools in the representative areas of Northwest China are selected as the research unit. The relevant research data are collected by issuing questionnaires, and the data set is constructed to verify the performance of the model. The experimental results show that the average prediction errors of the BPNN model and the support vector regression (SVR) model are 1.87 and 5.4, respectively. The error of BPNN is 65.4% lower than that of SVR, so BPNN has a better performance. The prediction results of the test set show that the actual error and the relative error of the BPNN model are controlled in the range of 0.01, and the prediction accuracy is high. The structural equation model has a high fitting degree. The results of the questionnaire analysis show that attachment, self-efficacy, and psychological anxiety exert a significant direct impact on mental elasticity. This exploration aims to conduct a micro investigation on the relationship among the three core variables (attachment, self-efficacy, and mental health) in the resilience research of children in plight, and analyze their resilience, to provide a theoretical basis for the resilience intervention design of vulnerable groups.
New Insights Into Mouthings: Evidence From a Corpus-Based Study of Russian Sign Language
Authors: Bauer Anastasia , Kyuseva Masha
Published: February 2022

While some aspects of mouthings have been previously investigated, many topics in the use of this cross-modal contact phenomenon in sign languages remain un(der)studied, and not much is known about mouthings in Russian Sign Language (RSL), in particular. This article examines various aspects of mouthings as these are used by native RSL signers and aims to contribute new insights into the use and origin of mouthings in this sign language. Based on novel data from the online RSL Corpus alongside additional elicited data, we describe the distribution, forms, functions and spreading patterns of mouthings. Our findings furthermore show that sign languages exhibit more extensive variation in the use of mouthings than has previously been thought. Moreover, we – thus far uniquely – describe mouthings also as a written-language-based contact phenomenon. This study has the potential to provide a better understanding of the nature of such contact-induced features as mouthings in sign languages in general and reveals a complex interplay of the modalities of signed, spoken and written languages.
The Impact of Adaptive Learning in Entrepreneurial Behavior for College Students
Authors: Yang Dan
Published: February 2022

Entrepreneurship of college students has always been a hot topic in families, schools and society. Massive studies aim to explore entrepreneurial behavior. However, under the condition of the 10% success rate of student entrepreneurship, the adverse impact of COVID-19 and the changed circumstance of domestic entrepreneurship, this exploration aims to study the factors that influence college students’ entrepreneurial behavior choices under the epidemic. First, through the retrieval of relevant literature and theoretical study, the variable factors that affect behavior choices are sorted and summarized. It is assumed that the factors that affect behavior choices are entrepreneurial motivation, entrepreneurial ability, willingness to behave, and entrepreneurial environment. Second, a questionnaire is designed to investigate the choice of entrepreneurial behavior for students who are starting a business or going to start a business. The standard effect values of the survey results are calculated by using structural equation modeling (SEM). The results reveal that the effect values of the nine hypothetical results are all in line with the prediction, which prove a positive impact of the four variable factors on the choice of entrepreneurial behavior. The experimental parameters set are as follows. The standardized effect value of Hypothesis 1 (entrepreneurial motivation has a positive impact on entrepreneurial behavior choice) is 0.216; that of Hypothesis 2 (entrepreneurial ability has a positive impact on the choice of entrepreneurial behavior) is 0.221; that of Hypothesis 3 (willingness to behave has a positive impact on entrepreneurial behavior choice) is 0.284; that of Hypothesis 4 (entrepreneurial environment has a positive impact on the choice of entrepreneurial behavior) is 0.329; that of Hypothesis 5 (entrepreneurial motivation has a positive impact on entrepreneurial intention) is 0.247; that of Hypothesis 6 (entrepreneurial ability has a positive impact on willingness to behave) is 0.339; that of Hypothesis 7 (entrepreneurial ability has a positive impact on entrepreneurial motivation) is 0.357; that of Hypothesis 8 (entrepreneurial environment has a positive impact on willingness to behave) is 0.336; that of Hypothesis 9 (entrepreneurial environment has a positive impact on entrepreneurial motivation) is 0.485. Besides, the entrepreneurial environment has the greatest impact on behavior choice. Therefore, it is believed that the government, society, schools need to strengthen the correct guidance of entrepreneurial students and create a good entrepreneurial environment to cope with economic changes under the epidemic.
Emotional Nuance: Examining Positive Emotional Granularity and Well-Being
Authors: Tan Tse Yen , Wachsmuth Louise , Tugade Michele M.
Published: February 2022

The focus of this review is on positive emotional granularity. Emotional granularity is the level of specificity that characterizes verbal representations of an affective experience. Although there has been research on negative emotional granularity, relatively less attention has been given to the study of positive emotional granularity. Positive emotions are theorized to motivate an individual to “broaden and build” one’s scope of cognition, attention, and behavior. Distinct positive emotion concepts may provide individuals with more informational value than that provided by global mood. Indeed, individuals who are higher in positive emotional granularity report being better at coping with stressful experiences. In this review, we discuss growing research on positive emotional granularity and well-being. Issues of measurement, interventions, and considerations for future lines of research are discussed.
Community Empowerment Under Powerful Government: A Sustainable Tourism Development Path for Cultural Heritage Sites
Authors: Hu Beiming , He Furong , Hu Lingshan
Published: February 2022

Community participation is the core of sustainable tourism development; however, it encounters obstacles at government-controlled heritage sites in China. This paper examines the status quo of community participation and residents’ empowerment perception through 25 in-depth interviews and 168 questionnaires in the Miao ethnic heritage site of Xijiang Village in southwest China, the findings reveal that: (a) The phenomenon of disempowerment focuses on the political and economic aspects, rather than the social and psychological aspects; (b) Spatial difference affects empowerment perception; and (c) Residents desire more political and education empowerment. On this basis, the present research puts forward a comprehensive empowerment system consists of system, information, and education empowerment which contributes to the theorization of community empowerment between Chinese and Western scholars and provides an alternative path to sustainable tourism development for developing countries’ cultural heritage sites.
Type D Personality Is an Independent Predictor of Lower Urinary Tract Symptoms in Young Men
Authors: Cheng Wei-Ming , Liou Ying-Jay , Fan Yu-Hua
Published: February 2022

This cross-sectional study, which included men aged 20–40 years, aimed to determine the relationships among type D personality, depressive symptoms and lower urinary tract symptoms in young men. An internet-based questionnaire was administered, and General demographics, International Prostate Symptom Scores, Type D Scale-14 scores, and Depression and Somatic Symptom Scale scores were analyzed. A total of 3,127 men were included; of these, 762 (24.4%) reported moderate/severe lower urinary tract symptoms, and 1,565 (50.05%) met the criteria for type D personality. Men with type D personality had significantly higher body mass index and total and sub-scores for the International Prostate Symptom Score and Depression and Somatic Symptom Scale. Furthermore, the type D personality group had a higher prevalence of lower urinary tract symptoms, particularly voiding symptoms. Univariate analysis revealed that all parameters, except for body mass index, were significant predictors of moderate/severe lower urinary tract symptoms. Multivariate analysis showed that age >30 years, type D personality, and depressive and somatic Depression and Somatic Symptom Scale sub-scores were independent predictors of moderate/severe lower urinary tract symptoms. Regarding Type D Scale-14 subscales, social inhibition, rather than negative affectivity, impacted moderate/severe lower urinary tract symptoms. Mediation analysis revealed that depressive symptoms mediated the relationship between type D personality and lower urinary tract symptoms. This study established correlations between type D personality, depressive symptoms, and lower urinary tract symptoms. As previous studies suggested that patients with type D personality are less likely to consult and adhere to treatment, and are at higher risk for depression, urologists should therefore actively recognize patients with TDP.
Charity Begins at Home: Understanding the Role of Corporate Social Responsibility and Human Resource Practices on Employees’ Attitudes During COVID-19 in the Hospitality Sector
Authors: John Albert , Shahzadi Gulnaz , Khan Kanwal Iqbal , Chaudhry Shafaq , Sarwar Bhatti Muhammad Arslan
Published: February 2022

The COVID-19 outbreak wreaked havoc on the hospitality business, resulting in significant layoffs, salary cuts, and unpaid leaves globally. This study uses the sensemaking theory to investigate how COVID-19 induced unfavorable human resource (HR) practices affect the link between perceived corporate social responsibility (CSR) and employee identification and commitment. We tested this model using the data collected from 392 hospitality sector employees in Pakistan. The results reveal that “cut in salaries” and “work from home” positively moderate CSR’s impact on employees’ identification and commitment. On the other hand, employee layoff and leave without pay do not impact the positive relationship between CSR and employees’ attitudes. Furthermore, the study finds that CSR during this pandemic has a significant positive impact on employees’ attitudes. However, this relationship becomes insignificant for employees who reported unfavorable HR practices in their organizations. The finding further reveals that CSR’s impact during COVID-19 on employees’ attitudes is moderated by the different levels of CSR importance in employees’ minds. This evidence is significant since HR practices implemented during this crisis need to be identified and framed to understand the effects of CSR on employee commitment and identification. CSR involvement in the pandemic can help managers keep their employees committed to organizations; only if this charity begins from their internal stakeholders first.
Hope for “Continued Vitality”: Qualitative Study of Adults With Traumatic Brain Injury and Low Mood on Their Rehabilitation
Authors: Chui Adora , Dainty Katie N. , Kirsh Bonnie , Dawson Deirdre R. , Colquhoun Heather
Published: February 2022

Objective

Depression is highly comorbid with traumatic brain injury (TBI) with often complex and interacting symptomology that contributes to the experience of disability. Comorbid depression results in poorer TBI rehabilitation and downstream participation outcomes yet perspectives of this group regarding person-centered care is unknown.

Purpose

This study aimed to explicate the perspectives of persons with TBI and depression on their values, preferences, and desired outcomes for optimal rehabilitation.

Methods

A qualitative descriptive approach was taken. Thirteen adults [mean age: 40.5 (standard deviation 9.8)] diagnosed with TBI and with self-reported low mood were recruited through convenience sampling. Participants were predominantly female (n = 12) with concussion/mild TBI and at least 6 months post-injury. One-on-one, semi-structured interviews were conducted by phone with Canadian participants (March-May 2020). Interviews were transcribed; data were analyzed thematically by two researchers and the thematic map refined by the research team.

Results

Three themes were identified on values, preferences, and desired outcomes in person-centered care. Participants valued “validation” from healthcare providers and the health system to feel seen and believed about their conditions and concerns. They preferred for healthcare providers to “share the burden of managing care” through improved interactions and better access to concussion care. Participants expressed that “meaningful outcomes” were to be symptom free, to resume valued life activities, and to be able to adapt/be resilient. The latter indicated hope for “continued vitality” for life participation despite past and ongoing challenges.

Conclusions

Many adults with TBI and self-identified low mood expressed rehabilitation experiences that were invalidating. Their identified values, preferences, and desired outcomes provide directions for better person-centered care by healthcare providers and health systems to support participation.
Rotation Periods of Asteroids Determined With Bootstrap Convex Inversion From ATLAS Photometry
Authors: Ďurech Josef , Vávra Michael , Vančo Radim , Erasmus Nicolas
Published: February 2022

The rotation period is one of the fundamental physical characteristics of asteroids. It can be determined from photometric measurements by standard methods of time-series period analysis or by creating a physical model of an asteroid with the rotation period being one of the fitted parameters. We used the latter approach to determine the sidereal rotation period for more than 5000 asteroids, out of which about 1600 are those for which their period was not known. We processed photometric measurements of about 100,000 asteroids from the ATLAS survey with the light curve inversion technique in the Asteroids@home project to search for the best-fit rotation period. This was repeated 25 times with randomly resampled—bootstrapped—data. For thousands of asteroids, their best-fit period was the same for most of the bootstrapped data sets; thus, their rotation period was determined with a high degree of reliability.
Case Report: Benign Uterine Adenomyoma Metastasis in the Right Lung
Authors: Zhang Xiaowei , Jiang Hongquan , Huang Bifei , Wei Hangping
Published: February 2022

Background

Pulmonary metastasis of benign uterine leiomyoma and uterine endometriosis has been reported; however, pulmonary benign metastasizing uterine adenomyoma has not been reported. Herein, we report the first case of pulmonary benign metastasizing uterine adenomyoma. It is very important to differentiate from pulmonary primary synovial sarcoma; histopathology and immunohistochemistry are very helpful, molecular pathology can be used if necessary.

Case Presentation

A female patient was admitted to the hospital because of pulmonary nodules. Lung computed tomography (CT) showed a nodular high density shadow in the upper lobe of the right lung, with a clear boundary and a diameter of approximately 1.2 cm. A contrast CT scan showed obvious enhancement, and no obvious lobulation or burr was found. Video-assisted thoracoscopic resection of the tumor was performed. The upper lobe nodules were completely removed. Postoperative pathological report confirmed the lesion as metastatic benign adenomyoma of the right upper lung.

Conclusion

The lung is the most common organ for malignant tumor metastasis, and a few benign tumors can also develop pulmonary metastasis. Pulmonary benign metastasizing adenomyoma is extremely rare, and the prognosis is very good after surgical resection. When pulmonary CT shows a solid high-density shadow, we should consider the possibility of a metastatic benign tumor.
Isolation, Identification, and Genomic Characterization of Chicken Astrovirus Isolates From China
Authors: Zhao Wei , Shi Jialei , Yao Yongxiu , Shao Hongxia , Qin Aijian , Qian Kun
Published: February 2022

Chicken astrovirus (CAstV) infection can cause diarrhea, nephritis, stunted growth, and “white chickens” condition, resulting in economic losses to the poultry industry. Currently, a few CAstVs were isolated and a few full-length genome sequences of CAstV have been deposited in the GenBank. In the present study, two CAstV isolates (AAstV/Chicken/CHN/2017/NJ01 and AAstV/Chicken/CHN/2018/CZ01) were successfully isolated by using LMH cells, and we molecularly characterized these two CAstV isolates and observed the effect of these two isolates on hatchability using chicken embryo infection experiment. The genetic analysis demonstrated that these two strains had the typical characteristics of avian astroviruses, which were composed of three open reading frames, 5′UTR, and 3′UTR. The full-length genome sequence showed a high-degree identity at nucleotide level of 97.5–98.7% among Chinese isolates suggesting their common ancestors and limited sequence divergence. Sequence analysis of ORF2, which encodes the capsid protein associated with classification of avian astrovirus, revealed our two isolates belonging to CAstV Bi subtype. At the amino acid level, the complete capsid region of Chinese strains shared genetic distances of 0.03–0.04 with FP3 strains isolated from the UK, suggesting their common origin. Meanwhile, hatchability reduction was observed. These results provided novel insights into the molecular epidemiology and hatchability effect of CAstV.
Effects of Hydroxylated Lecithin on Growth Performance, Serum Enzyme Activity, Hormone Levels Related to Lipid Metabolism and Meat Quality in Jiangnan White Goslings
Authors: Wu Hongzhi , Wang Sibo , Tian Yong , Zhou Ning , Wu Chunqin , Li Ruiqing , Xu Wenwu , Xu Tieshan , Gu Lihong , Ji Fengjie , Xu Li , Lu Lizhi
Published: February 2022

The objective of the present study was to evaluate the effects of hydroxylated lecithin on growth performance, serum enzyme activity, hormone levels related to lipid metabolism and meat quality in Jiangnan White goslings. Six hundred 1-day-old goslings were randomly divided into five treatments with six replicates and 20 for each replicate. The control group (CG) was fed the basal diet, while the experimental group was fed the basal diet with 50, 100, 200 mg/kg hydroxylated lecithin and 100 mg/kg soy lecithin (HLG50, HLG100, HLG200, and LG100, respectively) in the form of powder. Feed and water were provided ad libitum for 32 days. Compared with the CG, (a) the average daily feed intake was higher (P < 0.05) in HLG100, the final body weight and average daily gain were higher (P < 0.05), and the feed conversion ratio was lower in the HLG200; (b) the alanine aminotransferase, malate dehydrogenase, leptin, glucagon, thyroid hormone, Triiodothyronine contents in the HLG200 were lower (P < 0.05); (c) The breast muscle water holding capacity was higher (P < 0.05) in groups with hydroxylated lecithin, the breast muscle shear force and fiber diameter were lower (P < 0.05) in the HLG100; (d) the inositic acid, intramuscular fat, phospholipid contents were higher (P < 0.05), the triglyceride content was lower (P < 0.05) in HLG100 of the breast muscle; (e) the relative expression of sterol regulatory element-binding protein-1 genes were higher (P < 0.05) in the treated groups of muscles, the phosphorylase kinase gamma subunit 1 gene expression was shown an opposite trend. In comparison with LG100, (a) the feed conversion ratio was lower (P < 0.05) in HLG200; (b) the alanine aminotransferase and adiponectin contents were higher (P < 0.05), the malondialdehyde and free fatty acid contents were lower (P < 0.05) in HLG200; (c) the water holding capacity and intramuscular fat contents in the breast and leg muscles were higher (P < 0.05) in HLG200. The hydroxylated lecithin concentration of 200 mg/kg improved the growth performance, serum enzyme activity, hormone levels related to lipid metabolism, and the meat quality of Jiangnan White goslings.
Simulation of Runway Irregularities in a Novel Test Rig for Fully Electrical Landing Gear Systems
Authors: De Martin Andrea , Jacazio Giovanni , Sorli Massimo
Published: February 2022

The E-LISA research project, under way within the Clean Sky 2 framework, has the objective of developing an innovative iron bird dedicated to executing tests on the landing gear of a small aircraft transport equipped with an electro-mechanical landing gear and electrical brake. Such tests include the simulation of complete landing procedures under different operating conditions such as runway friction, presence of periodical defects along the runway, variable aircraft weight, and approach speed. To this end, the iron bird requires novel solutions in both its architecture and its control scheme. This paper details an innovative solution that is being implemented in the E-LISA iron bird to enable the execution of tests on a landing gear, reproducing the effects of any type of runway irregularity. First, the rig architecture is presented in detail, with particular care toward the hybrid position/force control system that manages its operations. Then, a simulation model is introduced with the objective of verifying the control system stability and the test rig capability to reproduce on the test articles the effects produced on the landing gear leg of periodical runway irregularities. Simulations results are presented, highlighting the stability of the proposed control scheme and providing a preliminary assessment of the system performances.
Time-Dependent Degradation of Naphthoquinones and Phenolic Compounds in Walnut Husks
Authors: Medic Aljaz , Zamljen Tilen , Hudina Metka , Veberic Robert
Published: February 2022

The aim of the present study was to investigate how individual phenolic compounds and phenolic groups in walnut husk gratings (e.g., naphthoquinones, flavanols, flavonols, hydroxycinnamic acids) are oxidized over time, with a particular focus on the juglone synthase pathway. Walnut husk gratings were prepared and left under ‘degradation’ conditions (exposure to the air, room temperature) at increasing times. Following methanol extraction of these husk gratings, the HPLC profile of methanolic extract of husk gratings exhibited twenty-six compounds over time, then hydrojuglone glucoside, α-hydrojuglone, and juglone were detailed by HPLC-mass spectrometry. Initially (0–20 min), the content of hydrojuglone glucoside in the husk gratings decreased by 40.4%, while the content of α-hydrojuglone increased by 20.0%, and then decreased. After an initial delay (0–20 min), juglone increased by 47.9% from 20 to 40 min, and then decreased. This initially confirmed that hydrojuglone glucoside and α-hydrojuglone could be considered as precursors of juglone. Different phenolic groups showed different degradation processes, although they all reached their highest content after 40 min. This might arise from degradation of the phenols, increased free phenols, or activation of the plant defense mechanism due to damage to the tissue, similar to the effects of stress or a pathogen attack. Although it has been reported that the phenolic compounds decrease when food is processed or damaged, they showed increases, which were not indefinite, but time dependent. As phenolic compounds are considered highly beneficial to human health, increases upon processing indicate the need for further investigations into healthier food preparation processes. This is the first study on the degradation pathways of juglone, using a mass spectrometer, in which we suggest that hydrojuglone glucoside and α-hydrojuglone are indeed the precursors of juglone. However, it is possible that there are other degradation pathways of hydrojuglone glucoside, since less juglone is synthesized than expected.
Early Motor Signs in Autism Spectrum Disorder
Authors: Posar Annio , Visconti Paola
Published: February 2022

A growing number of literature data suggest the presence of early impairments in the motor development of children with autism spectrum disorder, which could be often recognized even before the appearance of the classical social communication deficits of autism. In this narrative review, we aimed at performing an update about the available data on the early motor function in children with autism spectrum disorder. Early motor impairment in these children can manifest itself both as a mere delay of motor development and as the presence of atypicalities of motor function, such as a higher rate and a larger inventory, of stereotyped movements both with and without objects. In the perspective of a timely diagnosis, the presence of early motor signs can be an important clue, especially in an individual considered at high risk for autism. Motor and communication (both verbal and non-verbal) skills are connected and a pathogenetic role of early motor dysfunctions in the development of autism can be hypothesized. From this, derives the importance of an early enabling intervention aimed at improving motor skills, which could also have favorable effects on other aspects of development.
Is Rituximab-Associated Hypogammaglobulinemia Always Linked to B-Cell Depletion?
Authors: Damianaki Anthie , Tzanoudaki Marianna , Kanariou Maria , Liatsis Emmanouil , Panos Alexandros , Soldatou Alexandra , Kossiva Lydia
Published: February 2022

We describe a case of a 3-year-old male toddler with a history of severe and refractory warm antibody autoimmune hemolytic anemia (w-AIHA) since early infancy and hypogammaglobulinemia persisting 20 months after rituximab administration (second-line rescue therapy). Specifically, although peripheral blood flow cytometry B-cell population counts signified B-cell recovery following completion of rituximab therapy, IgG levels were barely detectable. Detailed laboratory evaluation did not reveal any humoral or cell-mediated immunity impairment and the patient remained asymptomatic, without any infections or recurrence of w-AIHA. Due to severe hypogammaglobulinemia, he was placed on immunoglobulin replacement therapy (IVIG). The implemented PID (primary immunodeficiency) gene panel identified only variants of uncertain significance (VUS). The aim of this report is to underline the documentation of persisting hypogammaglobulinemia after rituximab despite peripheral blood B-cell reconstitution.
Implications of Climate Change on Outdoor Recreation: The Case of National Parks in Israel
Authors: Teitler Regev Sharon , Palatnik Ruslana Rachel
Published: February 2022

Changing weather driven by climate change may influence tourists’ decisions about “when and where to go for vacation.” Indeed, the results of climate change have altered the attractiveness of a variety of destinations and locations, therefore changing the profitability of tourism-based businesses. The aim of the study was to evaluate the potential impact of climate change on visits to outdoor recreation sites. Specifically, the research assessed the impact of weather, as well as economic and other characteristics, on the number of domestic and international tourists visiting national parks located in different climate zones within a single country—Israel. This research was based on a unique database of actual daily visits by international and domestic tourists to national parks in Israel during a six-year period (2012–2017). Each national park has different accessibility characteristics and offers different attractions. The climate data included daily maximum temperature, rain, extreme weather, as well as temperature indices measuring heat and cold. The results of the econometric analysis showed that weather-related parameters have a statistically significant effect on national park visits among both domestic and international tourists, while the magnitude of the effect varies by park and visitors’ place of origin.
Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats
Authors: Wu Zhong , Zeng Wenzhi , Zhang Xun , Yang Jiangfan
Published: February 2022

A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns. Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→. The molecular weight was 3.9285 × 104 Da. The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis. The acidic tea polysaccharide could improve plasma and liver lipid metabolism. Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus. The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
Advanced Nanocomposite Hydrogels for Cartilage Tissue Engineering
Authors: Huang Jianghong , Liu Fei , Su Haijing , Xiong Jianyi , Yang Lei , Xia Jiang , Liang Yujie
Published: February 2022

Tissue engineering is becoming an effective strategy for repairing cartilage damage. Synthesized nanocomposite hydrogels mimic the structure of natural cartilage extracellular matrices (ECMs), are biocompatible, and exhibit nano–bio effects in response to external stimuli. These inherent characteristics make nanocomposite hydrogels promising scaffold materials for cartilage tissue engineering. This review summarizes the advances made in the field of nanocomposite hydrogels for artificial cartilage. We discuss, in detail, their preparation methods and scope of application. The challenges involved for the application of hydrogel nanocomposites for cartilage repair are also highlighted.
Efficacy of Single Tocilizumab Administration in an 88-Year-Old Patient with Severe COVID-19 and a Mini Literature Review
Authors: Ould Ouali Cid , Ladjouzi Nadia , Tamas Khidher , Raveloson Hendriniaina , Ben Hassen Jihene , El Omeiri Nesrine , Zouloumis Georges , Al Zoabi Mohamed Moataz , Asadi Muneer , Jhouri Aziza , Schlatter Joël
Published: February 2022

The new coronavirus disease 2019 (COVID-19) could be associated with elevated inflammatory cytokine levels, suggesting the involvement of cytokine release syndrome. This syndrome is characterized by release of interleukin 6 correlated with COVID-19 severity and mortality. Targeting IL-6 with Tocilizumab treatment could be a potential therapeutic option for old patients. We report the case of an 88-year-old man with COVID-19 disease who presented at the admission with anemia, fever, oxygen desaturation (92%), and inflammatory syndrome (C-reactive protein (CRP) at 182.5 mg/L; reference range <5.0 mg/L). After remaining CRP level increase (206.6 mg/L), Tocilizumab administration led to rapid clinical outcome and resolution of his inflammatory syndrome. This case report represents a supplementary data confirming the efficacy and safety of Tocilizumab for COVID-19 in elderly patients.
Effects of Light Intensity and Temperature on the Photosynthesis Characteristics and Yield of Lettuce
Authors: Zhou Jing , Li Pingping , Wang Jizhang
Published: February 2022

Lettuce is an important vegetable in horticulture, but information about the interactive effects of light and temperature on its photosynthetic characteristics was inadequate. This work investigated the effects of three temperatures (15/10 (T15), 23/18 (T23), and 30/23 °C (T30)) and five light treatments (100 (P100), 200 (P200), 350 (P350), 500 (P500), and 600 μmol·m−2·s−1 (P600)) on the light–response curves, chlorophyll content, and yield of lettuce. The results showed that the maximum photosynthetic rate, light saturation point, chlorophyll content, and yield of lettuce were all the highest at T23 compared with T15 and T30 under different light intensities. Under the same temperature conditions, the photosynthesis capacity and yield of lettuce in the P350 and P500 treatments at T15, P350, P500, and P600 treatments at T23 and P500, and P600 treatments at T30 were larger than other light treatments. The results suggested that temperature play had a more pronounced influence on photosynthesis and yield in lettuce, but the appropriate levels of light intensity improved its potential photosynthetic capacity and yield under different temperature conditions.
Sea Buckthorn Hippophae rhamnoides and Fruit Flies Rhagoletis batava: Search for Volatile Semiochemicals Involved in Pest Attraction
Authors: Blažytė-Čereškienė Laima , Būda Vincas , Apšegaitė Violeta , Radžiutė Sandra , Būdienė Jurga , Aleknavičius Dominykas , Mozūraitis Raimondas
Published: February 2022

Rhagoletis batava (Diptera: Tephritidae), is the most important pest of Hippophae rhamnoides fruits. For detection and monitoring of R. batava, traps supplied with nonspecific attractants are used. Thus, new, more specific attractants for environment-friendly pest control are needed. Such attractants could be fruit-related semiochemicals that are involved in the host location by flies. Behavioural Y-olfactometer tests revealed that R. batava males were attracted to ripe fruit odour, while females preferred unripe and semi-ripe fruits. Thermal desorption gas chromatography-mass spectrometry (GC-MS) analysis revealed substantial quantitative and qualitative changes in volatiles between unripe and ripe fruits. In the unripe fruit emission, 41 volatile compounds were isolated, whereas 64 compounds were sampled from the ripe fruits. The total amount of volatiles increased five times during the fruit ripening. Gas chromatography-electroantennogram detection (GC-EAD) and GC-MS analyses of the fruit headspace volatiles revealed at least 26 compounds in unripe and 27 compounds in ripe fruits eliciting antennal responses of R. batava for both sexes. The fruits of these two ripening stages differed qualitatively in the single EAD-active compound only, i.e., 3-methylbutyl 2-methylpropionate. Esters were the most abundant volatiles, composing 84% and 93% of EAD-active compounds in the emissions of unripe and ripe fruits, respectively. Based on the persistent EAG responses, 17 compounds were selected as the most promising candidates for kairomone attractants of the sea buckthorn pest R. batava.
Identification of New Sources of Resistance to Anthracnose Caused by Colletotrichum horii among Persimmon Germplasms
Authors: Guan Changfei , Hu Jie , Li Yongkuan , Che Qinghui , Yang Yong
Published: February 2022

Persimmon (Diospyros kaki) anthracnose, predominantly caused by Colletotrichum horii, is a destructive disease of persimmon. Thus, the evaluation of resistance resources is imperative for persimmon breeding and resistant variety deployment. In this study, the isolate from persimmon branches was identified as C. horii by using molecular and morphological characteristics. A total of 142 varieties were tested for anthracnose disease response by inoculation with the conidial suspension of C. horii. A significant variability was observed among the studied accessions. Only two accessions, which had a hypersensitive reaction with a slight lesion at the infection site at 14 days post inoculation, were highly resistant to anthracnose. A total of 7 and 24 accessions exhibited resistant and susceptible responses, respectively, to anthracnose. A total of 109 varieties with the highest proportion (76.76%) of accessions showed high susceptibility to C. horii. A total of 43 persimmon main cultivars were selected for the re-evaluation of the resistance level by counting the natural disease incidence of branches and fruits that showed similar resistant response. Moreover, nine of highly resistant or resistant accessions possessed desirable agronomic characters, including high fruit weight and strong growth potential. These resistant accessions could be used in the breeding of anthracnose-resistant persimmon varieties.
The Differential Response of Intracellular Water Metabolism Derived from Intrinsic Electrophysiological Information in Morus alba L. and Broussonetia papyrifera (L.) Vent. Subjected to Water Shortage
Authors: Yu Rui , Wu Yanyou , Xing Deke
Published: February 2022

Plant electrical signals can quickly respond to the shifting environment. Almost all life activities of plants are dependent on water. The measurement of plant electrophysiological indices provides a more convenient method for studying the intracellular water utilization. In this study, Morus alba L. (Morus alba or M. alba) and Broussonetia papyrifera (L.) Vent. (Broussonetia papyrifera or B. papyrifera) were experimental materials, and the parameters were measured in two habitats (waterfront, well-water and arid slopes, deficient-water). The physiological and electrophysiological responses of leaves to different habitats were analyzed. The theoretically intrinsic relationships between the clamping force and leaf impedance (Z), capacitive reactance (Xc), resistance (R), and inductive reactance (Xl) were revealed as 3-parameter exponential decay and linear models based on bioenergetics, respectively. Leaf intrinsic electrophysiological parameters were successfully obtained by using the above-mentioned relationships and were used to manifest metabolic activity in plants. The intracellular water-holding capacity (IWHC), water use efficiency (IWUE), water-holding time (IWHT), and water transfer rate (WTR) of plant leaves were defined based on the intrinsic electrophysiological parameters and were used to reflect the intracellular water metabolism. The correlation between the physiological and electrophysiological parameters of the two plant species in the two habitats was also analyzed. The results showed that Morus alba continuously adapted to the shifting environment, the intracellular water metabolism was insensitive to soil water shortage and was independent from the external physiological state. The intracellular water metabolism in Broussonetia papyrifera was very sensitive to soil water shortage, and both intracellular water metabolism and immediate physiological parameters could characterize the response of Broussonetia papyrifera growth and development to soil water.
Effects of Integrated and Organic Management on Strawberry (cv. Camarosa) Plant Growth, Nutrition, Fruit Yield, Quality, Nutraceutical Characteristics, and Soil Fertility Status
Authors: Roussos Peter Anargyrou , Triantafillidis Athanassios , Kepolas Evaggelos , Peppas Panagiotis , Piou Anastassia , Zoti Maria , Gasparatos Dionisios
Published: February 2022

Strawberry plants (cv. Camarosa) were cultivated under organic (ORG) and integrated management (INT) practices to assess possible differences in plant growth, fruit production and quality, soil fertility, and plant nutrition. Two integrated fertilizer schemes were used, differentiating based on the origin of the major organic fertilizer used, i.e., chicken manure (I-ACT) versus fermented fungal biomass (I-AGR). Plant growth was higher under I-ACT treatment, whereas leaf area did not differ among treatments. The two integrated schemes resulted in higher fruit production. The organoleptic characteristics of the fruits did not differ, and this was also confirmed by a taste panel. The antioxidant capacity and flavanol concentration of organically produced fruits were higher, as well as fruit carbohydrate and total organic acid concentration. Leaf nitrogen concentration was higher under I-ACT, followed by ORG. I-ACT resulted in high leaf potassium concentration, as well as iron and manganese (together with I-AGR). Under ORG management, soil EC was found to be lower, whereas soil available phosphorus concentration was highest. Discriminant analysis taking into account fruit quality and phytochemical properties distinguished ORG management from the two integrated management practices, and when soil properties and leaf nutrient concentration were considered; all three systems were separated, indicating that the type of fertilizers used plays a significant role.
Inferring Hydrological Information at the Regional Scale by Means of δ18O–δ2H Relationships: Insights from the Northern Italian Apennines
Authors: Cervi Federico , Tazioli Alberto
Published: February 2022

We compared five regression approaches, namely, ordinary least squares, major axis, reduced major axis, robust, and Prais–Winsten to estimate δ18O–δ2H relationships in four water types (precipitation, surface water, groundwater collected in wells from lowlands, and groundwater from low-yield springs) from the northern Italian Apennines. Differences in terms of slopes and intercepts of the different regressions were quantified and investigated by means of univariate, bivariate, and multivariate statistical analyses. We found that magnitudes of such differences were significant for water types surface water and groundwater (both in the case of wells and springs), and were related to robustness of regressions (i.e., standard deviations of the estimates and sensitiveness to outliers). With reference to surface water, we found the young water fraction was significant in inducing changes of slopes and intercepts, leading us to suppose a certain role of kinetic fractionation processes as well (i.e., modification of former water isotopes from both snow cover in the upper part of the catchments and precipitation linked to pre-infiltrative evaporation and evapotranspiration processes). As final remarks, due to the usefulness of δ18O–δ2H relationships in hydrological and hydrogeological studies, we provide some recommendations that should be followed when assessing the abovementioned water types from the northern Italian Apennines.