bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of 10-Week Online Moderate- to High-Intensity Interval Training on Body Composition, and Aerobic and Anaerobic Performance during the COVID-19 Lockdown
Authors: Rodríguez-García Lorena , Ceylan Halil Ibrahim , Silva Rui Miguel , Silva Ana Filipa , Guadalupe-Grau Amelia , Liñán-González Antonio
Published: December 2023

The present study aimed to investigate the effects of a 10-week online high-intensity interval training (HIIT) program on body composition and aerobic and aerobic performance in physically sedentary women. A parallel, two-group, longitudinal (pre, post) design was used with physical tests performed before (preintervention) and after (postintervention) the 10-week intervention period. A total of forty-eight healthy and physically sedentary women (defined as an individual who lacks regular exercise or a structured fitness routine) were recruited to participate in this study. The participants were distributed in two groups: the experimental group (EG) with 24 women (mean ± SD: age 21.21 ± 2.15 years; weight: 61.16 ± 8.94 kg; height: 163.96 ± 4.87 cm; body mass index (BMI): 22.69 ± 2.49 kg/m2) and the control group (CG) with another 24 women (mean ± SD: age 20.50 ± 1.29 years; weight: 62.0 ± 6.65 kg; height: 163.92 ± 4.89 cm; body mass index: 23.04 ± 1.74 kg/m2). The EG performed an online HIIT program for 10 weeks, while the CG continued with their daily life routines. The repeated measures ANCOVA indicated a significant effect in the within-group analysis for weight (p = 0.001; d = −0.96) and for BMI (p = 0.001; d = 0.24), with a significant decrease in the experimental group (EG). The control group (CG) did not show any significant decrease in either body weight or BMI. Regarding the maximal oxygen uptake (VO2 max) values, the EG exhibited a significant improvement (p = 0.001; d = −1.07), whereas the CG did not demonstrate a significant improvement (p = 0.08; d = −0.37). The EG’s power output (W) (p = 0.001; d = −0.50) and power output standardized by body weight (W/kg) (p = 0.001; d = −0.96) were significantly improved. The CG did not show a significant improvement in either power output (W/kg) or power output. Lastly, the within-group analysis with load revealed that the EG significantly improved (p = 0.001; d = −0.50), while CG did not show a significant improvement in load (p = 0.10.; d = −0.10). The present study showed that 10 weeks of HIIT in an online environment during the COVID-19 lockdown significantly improved maximum oxygen consumption and caused weight loss and a significant decrease in body mass index in physically sedentary women. These results suggest that HIIT may be used as a time-efficient strategy to improve body composition and cardio-respiratory fitness in sedentary women.
Disparities in Healthcare and HBV Vaccination by Smoking Status: Findings from the National Health and Nutrition Examination Survey (NHANES) 2017–2018
Authors: Lin Wenxue
Published: December 2023

Cigarette smokers face greater challenges in accessing healthcare compared with non-smokers. In the US, approximately 2.2 million individuals are chronically infected with hepatitis B virus (HBV). I used data from the National Health and Nutrition Examination Survey (NHANES) 2017–2018 to investigate the association between smoking status (current, former, and never smoker) and different health outcomes, including healthcare accessibility, HBV vaccination, general health condition, and health insurance. Multivariable logistic regressions were used to analyze healthcare disparity by smoking status. I found that current smokers had 40% higher odds (AOR = 1.4, 95% CI: 1.1, 1.8) of lacking routine healthcare access compared with non-smokers. Regardless of smoking status, I observed a high rate of HBV non-vaccination among all participants. Specifically, 64% of current smokers, 67% of former smokers, and 57% of non-smokers had not received a single dose of HBV immunization. My study sheds light on the persisting gaps in healthcare access, particularly for smokers, and the urgent need to promote awareness and vaccination against hepatitis B.
Mental Health Awareness and Promotion during the First 1000 Days of Life: An Expert Consensus
Authors: Wardoyo Hasto , Moeloek Nila , Basrowi Ray , Ekowati Maria , Samah Kristin , Mustopo Widura , Nurdjasmi Emi , Widyahening Indah , Medise Bernie , Darus Febriansyah , Sundjaya Tonny , Pelangi Bunga
Published: December 2023

The first 1000 days of life constitute a critical phase that will determine the optimum growth and development of a child. An important factor in this phase of life is the perinatal mental health of mothers and children. Mental health awareness is an important public health issue with significant impacts on mothers, spouses, and families, as well as the long-term emotional and cognitive development of children as well. However, the awareness and promotion of mental health within the realms of reproductive health, maternal health, and infant health, i.e., the first 1000 days of life, do not receive high prioritization in Indonesia. Nonetheless, Indonesia, with its existing primary healthcare system, has the potential to raise awareness of and promote the importance of perinatal mental health for its citizens. This experts’ consensus proposes several strategies to maximize the usefulness of primary healthcare facilities in Indonesia, including Community Health Centers and Integrated Healthcare Posts, to support perinatal mental health awareness and promotion during the first 1000 days of life. The success of this program, in return, will improve the health status of women and children in Indonesia.
Nosocomial Coronavirus Disease 2019 during 2020–2021: Role of Architecture and Ventilation
Authors: Martinot Martin , Mohseni-Zadeh Mahsa , Gravier Simon , Ion Ciprian , Eyriey Magali , Beigue Severine , Coutan Christophe , Ongagna Jean-Claude , Henric Anais , Schieber Anne , Jochault Loic , Kempf Christian
Published: December 2023

Nosocomial coronavirus disease 2019 (COVID-19) is a major airborne health threat for inpatients. Architecture and ventilation are key elements to prevent nosocomial COVID-19 (NC), but real-life data are challenging to collect. We aimed to retrospectively assess the impact of the type of ventilation and the ratio of single/double rooms on the risk of NC (acquisition of COVID-19 at least 48 h after admission). This study was conducted in a tertiary hospital composed of two main structures (one historical and one modern), which were the sites of acquisition of NC: historical (H) (natural ventilation, 53% single rooms) or modern (M) hospital (double-flow mechanical ventilation, 91% single rooms). During the study period (1 October 2020 to 31 May 2021), 1020 patients presented with COVID-19, with 150 (14.7%) of them being NC (median delay of acquisition, 12 days). As compared with non-nosocomial cases, the patients with NC were older (79 years vs. 72 years; p < 0.001) and exhibited higher mortality risk (32.7% vs. 14.1%; p < 0.001). Among the 150 NC cases, 99.3% were diagnosed in H, mainly in four medical departments. A total of 73 cases were diagnosed in single rooms versus 77 in double rooms, including 26 secondary cases. Measured air changes per hour were lower in H than in M. We hypothesized that in H, SARS-CoV-2 transmission was favored by short-range transmission within a high ratio of double rooms, but also during clusters, via far-afield transmission through virus-laden aerosols favored by low air changes per hour. A better knowledge of the mechanism of airborne risk in healthcare establishments should lead to the implementation of corrective measures when necessary. People’s health is improved using not only personal but also collective protective equipment, i.e., ventilation and architecture, thereby reinforcing the need to change institutional and professional practices.
Investigating Differences in Personality Traits, Self-Esteem, Eating Attitudes, and Body Image among Participants in Indoor and Outdoor Fitness Activities
Authors: Tsartsapakis Ioannis , Chalatzoglidis Georgios , Zafeiroudi Aglaia
Published: December 2023

Physical activity environments influence physical condition, nutrition, individual and social behaviors, and emotional well-being. The aim of the study was to investigate differences in eating attitudes, self-esteem, personality traits, and body image among participants in indoor and outdoor leisure-time fitness programs. Participants included 1747 adults (882 men and 865 women) aged 34 ± 9.2 years, divided into experimental and control groups. All participants completed the Demographic Characteristics Questionnaire, the Eating Attitudes Test 26 (EAT-26), the Self-Esteem Scale (RSES), the Traits Personality Questionnaire 5 (TPQue5), and the Appearance Scales of the Multidimensional Body–Self Relations Questionnaire (MBSRQ-AS). The results revealed statistically significant differences between groups. One-way analysis of variance (ANOVA) revealed noteworthy variations in personality trait scores between the groups (neuroticism, extraversion, and conscientiousness) as well as body image factors (self-esteem and eating attitudes). Two-way ANOVA revealed a significant interaction between gender and participation in different types of exercise. Females had lower self-esteem and body area satisfaction scores but higher eating disorder proneness, neuroticism, appearance evaluation, and overweight preoccupation scores than males. These findings add to the current literature focusing on the psychosocial and behavioral mechanisms associated with physical activity and exercise environments. The findings provide an effective supplement to promote public health-focused fitness programs and leisure-time physical activity motivation strategies.
X-ray-Based 3D Histology of Murine Hearts Using Contrast-Enhanced Microfocus Computed Tomography (CECT) and Cryo-CECT
Authors: Pestiaux Camille , Marino Alice , Simal Lauriane , Horman Sandrine , Capoulade Romain , Kerckhofs Greet
Published: December 2023

Cardiovascular diseases are the most common cause of death worldwide, and they still have dramatic consequences on the patients’ lives. Murine models are often used to study the anatomical and microstructural changes caused by the diseases. Contrast-enhanced microfocus computed tomography (CECT) is a new imaging technique for 3D histology of biological tissues. In this study, we confirmed the nondestructiveness of Hf-WD 1:2 POM-based CECT and cryogenic CECT (cryo-CECT) to image the heart in 3D. The influence of the image quality (i.e., acquisition time and spatial resolution) was assessed for the characterization of the heart structural constituents: heart integrity, the coronary blood vessels and the heart valves. Coronary blood vessels were visualized and segmented in murine hearts, allowing us to distinguish veins from arteries and to visualize the 3D spatial distribution of the right coronary artery and the left main coronary artery. Finally, to demonstrate the added value of 3D imaging, the thickness distribution of the two leaflets in the mitral valve and three cusps in the aortic valve was computed in 3D. This study corroborates the added value of CECT and cryo-CECT compared to classical 2D histology to characterize ex vivo the structural properties of murine hearts and paves the way for the detailed 3D (micro)structural analyses of future cardiovascular disease models obtained in mice and rats.
Climate Change and Pilgrimage to Shrines in Ethiopia
Authors: Brimblecombe Peter , Tola Habtamu Gizawu , Richards Jenny
Published: December 2023

Pilgrimages are an important part of our intangible heritage. These long journeys, often on foot, can be sensitive to weather, so this study sees pilgrimages as providing an opportunity to look at the way in which changes in climate affect intangible heritage. It examines two important Ethiopian pilgrimages that involve hundreds of thousands who travel each year to Dirre Sheikh Hussein, seen as the country’s Mecca, and Lalibela, its Jerusalem. These journeys in the cold season (December–February) often exceed 1000 km in length and expose pilgrims to low temperatures in mountain areas. Our analysis uses daily output data from ERA-5 and CHIRPS for rainfall and temperature across the recent past (1984–2014) and an ensemble of climate models (CMIP6) for the periods 1984–2014 and 2035–2065, to explore changes in nighttime low temperature, daytime high temperature and the potential increase in days of heavy rain in mountain areas. Additionally, we examine the increasing number of very hot days affecting travel to and from Dirre Sheikh Hussein. The pilgrims experience weather events and not long-term average conditions, so extremes and spells of inclement weather can affect their experience. Management plans for the regions have yet to address likely changes to climate at these religious sites, or consider how strategic planning might mitigate their impact on pilgrims.
Life Cycle Assessment in Protected Agriculture: Where Are We Now, and Where Should We Go Next?
Authors: Villagrán Edwin , Romero-Perdomo Felipe , Numa-Vergel Stephanie , Galindo-Pacheco Julio Ricardo , Salinas-Velandia Diego Alejandro
Published: December 2023

Researchers and practitioners use life cycle assessment (LCA) as a powerful tool to thoroughly assess the environmental impact of protected agriculture. However, the literature in this field has shown heterogeneity, which is characterized by inconsistent methodologies and assumptions. Identifying prevailing trends and resolving existing limitations is necessary to generate robust results and guide future work. Here, we conduct a bibliometric and systematic review to explore how LCA applications have addressed protected agriculture. The bibliometric analysis unveils trends in scientific productivity, spanning temporal evolution and geographic distribution, while also identifying prominent research avenues. The systematic review traces the historical trajectory of agricultural LCA and scrutinizes methodological decisions across the standard LCA phases: (i) objective and scope, (ii) life cycle inventory, (iii) impact assessment, and (iv) interpretation. We summarize and discuss the reported environmentally friendly practices and provide a qualitative interpretation of the LCA findings. Moreover, we pinpoint key methodological challenges and propose research horizons. It is crucial to note that the environmental benefits of protected agriculture are context-dependent, with climate change emerging as a critical factor influencing crop yields and the system’s input and output resources. This impact is particularly pronounced in terms of water and energy consumption and carbon emissions. In regions with extreme climates, protected agriculture provides solutions for producers aiming to attain high yields of top-quality crops. The integration of circular bioeconomy strategies in this context allows mitigation of the environmental trade-offs identified by LCA.
Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime
Authors: Marković Marija , Trifunović-Momčilov Milana , Radulović Olga , Paunović Danijela , Antonić Reljin Dragana , Jevremović Slađana , Uzelac Branka
Published: December 2023

The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.
The CDPK Gene Family in Mustard (Brassica juncea L.): Genome-Wide Identification and Expression Analysis under Cold Stress
Authors: Li Haibo , Wu Hao , Huang Weifeng , Liu Jiaxian , Deng Jiaxin , Li Chuanhong , Mao Cui , Zhang Yang , Wang Yukun , Zheng Jie
Published: December 2023

The CDPK family genes play crucial roles in signal transduction pathways during plant development and stress response. In this study, we comprehensively analyzed the CDPK family genes in mustard (Brassica juncea L.), resulting in the identification of 101 genes (BjuCDPK1–101) located on chromosomes AA_Chr01 to BB_Chr08. RNA-seq analysis showed that most BjuCDPK genes were predominantly expressed in the root and flower bud, suggesting their organ-specific expression patterns. Furthermore, the expression levels of most BjuCDPK genes were significantly altered by cold stress. The promoter regions of BjuCDPK genes had abundant cis-acting elements related to hormones and stress. The interaction network analysis predicted that BjuCDPK proteins respond to abiotic stress probably via interaction with ABF1, ABF4, and/or DI19. Our findings provide valuable information for further the investigation of the cold stress adaption of mustard via the CDPK signaling pathway.
Establishment of an In Vitro Micropropagation Protocol for Hibiscus moscheutos L. ‘Berry Awesome’
Authors: Sereda Mikhail , Petrenko Victoria , Kapralova Olga , Chokheli Vasily , Varduni Tatyana , Dmitriev Pavel , Minkina Tatiana , Sushkova Svetlana , Barbashev Andrey , Dudnikova Tamara , Singh Rupesh Kumar , Seth Chandra Shekhar , Rajput Vishnu D.
Published: December 2023

Hibiscus moscheutos L. ‘Berry Awesome’ is a complex hybrid of the new Proven Winners Summerific series of varieties with highly ornamental characteristics. Micropropagation of highly ornamental varieties is important for mass production of planting material for commercial purposes. The traditional methods for propagating Hibiscus varieties, such as cuttings or seed propagation, however, do not guarantee high rates of production of high-quality seedlings. To solve this problem, an attempt was made to develop protocols for micropropagation of Hibiscus moscheutos L. ‘Berry Awesome’ in vitro on agar and liquid medium using a bioreactor system, followed by ex vitro adaptation of the regenerants. The optimal method for sterilization of nodal explants as well as the optimal composition of the initiation medium for shoot proliferation and rooting were determined. For micropropagation on a liquid medium, a rocker-type bioreactor was used, and its advantages over micropropagation on an agar medium were demonstrated. The results showed that the best sterilization method for nodal segment explants was as follows: pretreatment by rinsing with running tap water, sterile water, and distilled water for 70 min and soaking for 5 min in a mixture of solutions of ethyl alcohol (96%), hydrogen peroxide (38%), and water in a ratio of 1:1:2. In this case, live and sterile explants accounted for 62.6%. The optimal initiation medium for axillary buds in nodal segments was the Murashige and Skoog (MS) medium supplemented with 0.1 mg L−1 N-(2-chloro-4-pyridinyl)-N’-phenylurea (CPPU), which resulted in 73.3% of axillary buds being induced. The optimal solid proliferation medium was MS medium supplemented with 0.1 mg L−1 CPPU with a proliferation coefficient of 5.8. In a liquid medium, the optimal concentration of CPPU was 0.05 mg L−1 with a proliferation coefficient of 9.2. The best medium for rooting/shoots with agar and in bioreactors was MS medium with the addition of 0.1 mg L−1 indole-3-butyric acid (IBA). The highest rooting rate was 99.0% in both types of media, and the survival rate of plantlets was 88.7% in solid media and 98.7% in the bioreactor.
From Fresh to Salt Waters: First Reports of the Red Swamp Crayfish Procambarus clarkii (Girard, 1852) in Mediterranean Marine Waters
Authors: Nota Alessandro , Santovito Alfredo , Gattelli Raffaele , Tiralongo Francesco
Published: December 2023

The red swamp crayfish Procambarus clarkii (Girard, 1852), native to the southern/southeastern United States and northern Mexico, has been introduced in many countries worldwide. The species has a remarkable invasiveness due to its high reproductive rate and ability to tolerate broad ranges of water parameters like salinity. Nevertheless, no consistent observations of P. clarkii have been reported in the marine environment until now. Data demonstrating the presence of the crayfish within marine ecosystems were collected by a personal acquaintance and two volunteers who published photos of the species on Facebook marine biology and fishing groups. The species was identified based on photos taken by these people in the described areas. P. clarkii has been spotted in three different localities, in two of which observations are recurrent. The species can survive miles from the nearest freshwater stream and could potentially colonise marine waters at depths up to 20 m. The multiple observations of P. clarkii collected in our study uncover a persistent presence of the species within some marine areas, rather than its mere sporadic occurrence. Our results highlight the need for further investigation to obtain a comprehensive understanding of the crayfish’s ability to withstand salinity and colonise the marine environment.
The Potential of Isotopic Tracers for Precise and Environmentally Clean Stream Discharge Measurements
Authors: Picard Antoine , Barbecot Florent , Bardoux Gérard , Agrinier Pierre , Gillon Marina , Corcho Alvarado José A. , Schneider Vincent , Hélie Jean-François , de Oliveira Frédérick
Published: December 2023

Accurate discharge measurement is mandatory for any hydrological study. While the “velocity” measurement method is adapted to laminar flows, the “dilution” method is more appropriate for turbulent streams. As most low-gradient streams worldwide are neither laminar nor turbulent, a methodological gap appears. In this study, we demonstrate that the application of the “dilution” method to a low-gradient small stream gives very satisfactory results in addition to revealing surface/subsurface processes. A variety of chemical and isotopic tracers were injected into the stream (anions, fluorescent dyes, and chloride and hydrogen isotopes). We report the first use of 37Cl for stream discharge measurement and show that 37Cl and 2H can be reliably used as quantitative tracers. Discharge uncertainty calculations show that deuterium is the most accurate tracer method used. We also compare the differences in the tailing part of the restitution curves of tracers and investigate the role of transient surface and hyporheic zones in solute transport in light of a simple transport modelling approach. We conclude that isotopic tracers can be used as “environmentally friendly” tracers for discrete stream discharge measurements and that the application of multi-tracers tests in rivers opens the path to a better understanding of surface–subsurface interaction processes.
Severe Post-COVID-19 Condition after Mild Infection: Physical and Mental Health Eight Months Post Infection: A Cross-Sectional Study
Authors: Egger Marion , Vogelgesang Lena , Reitelbach Judith , Bergmann Jeannine , Müller Friedemann , Jahn Klaus
Published: December 2023

Severe acute COVID-19 infections requiring intensive care treatment are reported risk factors for the development of post-COVID-19 conditions. However, there are also individuals suffering from post-COVID-19 symptoms after mild infections. Therefore, we aimed to describe and compare the health status of patients who were initially not hospitalized and patients after critical illness due to COVID-19. The outcome measures included health-related quality of life (EQ-5D-5L, visual analogue scale (VAS)); mental health (hospital anxiety and depression scale (HADS)); general disability (WHODAS-12); and fatigue (Fatigue-Severity-Scale-7). Individuals were recruited at Schoen Clinic Bad Aibling, Germany. A total of 52 non-hospitalized individuals (47 ± 15 years, 64% female, median 214 days post-infection) and 75 hospitalized individuals (61 ± 12 years, 29% female, 235 days post-infection) were analyzed. The non-hospitalized individuals had more fatigue (87%) and anxiety (69%) and a decreased health-related quality of life (VAS 47 ± 20) compared to the hospitalized persons (fatigue 45%, anxiety 43%, VAS 57 ± 21; p < 0.010). Severe disability was observed in one third of each group. A decreased quality of life and disability were more pronounced in the females of both groups. After adjusting for confounding, hospitalization did not predict the burden of symptoms. This indicates that persons with post-COVID-19 conditions require follow-up services and treatments, independent of the severity of the acute infection.
Attachment, Resilience and Life Satisfaction of University Students in Cyprus after the Fourth Wave of COVID-19
Authors: Parpottas Panagiotis , Vogazianos Paris , Pezirkianidis Christos
Published: December 2023

The COVID-19 pandemic has given rise to a large-scale crisis that has also impacted the well-being and, more specifically, the life satisfaction of university students. Factors such as attachment dimensions and psychological resilience can provide us with a better understanding of students’ life satisfaction levels during the recent pandemic. While previous literature has revealed a significant association between attachment dimensions, resilience, and life satisfaction, very few studies have attempted to address a more complex relationship among all three variables for university students, and even fewer have explored this topic during the COVID-19 pandemic. Therefore, the aim of the current study was to investigate the impact of attachment dimensions on university students’ life satisfaction after the fourth wave of COVID-19 in Cyprus, with a focus on the mediating role of psychological resilience. The sample comprised 780 university students, consisting of 323 men and 457 women, aged between 18 and 61 years. Participants were recruited electronically, and after being directed to Google Forms, they completed the ECR-R for their attachment dimensions, the RES for psychological resilience, and the SWLS for life satisfaction. The results indicated significant correlations between attachment dimensions, psychological resilience, and life satisfaction. Notably, psychological resilience was found to partially mediate the relationship between attachment anxiety, as well as attachment avoidance, and life satisfaction. Specifically, attachment anxiety and avoidance negatively affected life satisfaction, partially due to lower levels of psychological resilience. These findings are discussed in relation to the existing literature, and implications for practice are provided.
Anxiety and Worry about Six Categories of Climate Change Impacts
Authors: Stewart Alan E. , Chapman Harrison E. , Davis Jackson B. L.
Published: December 2023

The occurrence of severe and extreme weather events that have been attributed to a changed climate system and the widespread dissemination of the impacts of these events in the media can lead people to experience concern, worry, and anxiety, which we examined in two studies. In Study 1, we observed that people more frequently expressed worry than anxiety about the impacts of climate change in six areas. People were more frequently worried and anxious about the effects of climate change on future generations and about societal responses (or lack of a response) to climate change. The levels of anxiety that people expressed were significantly higher than the worry people reported when anxiety was their modal response. In Study 2, we observed that both climate change worry and anxiety were negatively correlated with psychological distance from climate change. Overall, climate change worry and psychological distance significantly predicted climate-sustainable behaviors. Our study was among the first to use developed measures of climate change worry, anxiety, and psychological distance to examine peoples’ responses across some of the possible impact and consequence areas of climate change.
Environmental Injustice and Electronic Waste in Ghana: Challenges and Recommendations
Authors: Njoku Anuli , Agbalenyo Martin , Laude Janaya , Ajibola Taiwo Folake , Attah Mavis Asiwome , Sarko Samuel Bruce
Published: December 2023

Electronic waste (e-waste) or discarded electronic devices that are unwanted, not working, or have reached their end of life pose significant threats to human and environmental health. This is a major concern in Africa, where the majority of e-waste is discarded. In the year 2021, an estimated 57.4 million metric tons of e-waste were generated worldwide. Globally, COVID-19 lockdowns have contributed to increased e-waste generation. Although Africa generates the least of this waste, the continent has been the dumping ground for e-waste from the developed world. The flow of hazardous waste from the prosperous ‘Global North’ to the impoverished ‘Global South’ is termed “toxic colonialism”. Agbogbloshie, Ghana, an e-waste hub where about 39% of e-waste was treated, was listed among the top 10 most polluted places in the world. The discard of e-waste in Ghana presents an issue of environmental injustice, defined as the disproportionate exposure of communities of color and low-income communities to pollution, its associated health and environmental effects, and the unequal environmental protection provided through policies. Despite the economic benefits of e-waste, many civilians (low-income earners, settlers, children, and people with minimal education) are exposed to negative health effects due to poverty, lack of education, and weak regulations. We critically examine the existing literature to gather empirical information on e-waste and environmental injustice. Comprehensive policies and regulations are needed to manage e-waste locally and globally.
The Effect of Social Isolation, Loneliness, and Physical Activity on Depressive Symptoms of Older Adults during COVID-19: A Moderated Mediation Analysis
Authors: Jin Min Jin , Bae Sung Man
Published: December 2023

Depressive symptoms have been commonly reported in older adults during the coronavirus disease (COVID-19) pandemic. Social isolation as a result of social distancing to prevent COVID-19 was reported to increase the level of depressive symptoms both directly and indirectly through the mediating effect of loneliness. Depressive symptoms in older adults can be regulated through health behaviors such as physical activity. Therefore, this study used a moderated mediation model to explain depressive symptoms. The English Longitudinal Study of Ageing COVID-19 wave 2 data were used. A total of 296 individuals were excluded due to missing values, leaving a final sample of 6499. Depressive symptoms, social isolation, loneliness, and physical activity were used in the moderated mediation analysis with various demographic and general health variables as covariates. Loneliness was found to significantly mediate the relationship between social isolation and depression. While moderate physical activity significantly moderated the effects of social isolation and loneliness on depressive symptoms, mild physical activity alone significantly moderated the effects of loneliness on depressive symptoms. This study revealed the impact of social isolation on depressive symptoms directly and indirectly mediated by loneliness, with a moderating effect of moderate and mild physical activity in the elderly during COVID-19 in a moderated mediation model.
A Quantitative and Qualitative Program Evaluation of a Case-Area Targeted Intervention to Reduce Cholera in Eastern Democratic Republic of the Congo
Authors: Endres Kelly , Mwishingo Alain , Thomas Elizabeth , Boroto Raissa , Ntumba Nyarukanyi Wivine , Bisimwa Jean-Claude , Sanvura Presence , Perin Jamie , Bengehya Justin , Maheshe Ghislain , Cikomola Cirhuza , George Christine Marie
Published: December 2023

Individuals living near cholera patients have an increased risk of cholera infections. Case-area targeted interventions (CATIs) promoting improved water, sanitation, and hygiene (WASH) present a promising approach to reducing cholera for those residing near cholera cases. However, there is limited evidence on the effectiveness and implementation of this approach in increasing WASH behaviors. We conducted a mixed-methods program evaluation in rural and urban eastern Democratic Republic of the Congo. The quantitative component included household structured observations and spot checks in CATI and control areas to assess WASH conditions and behaviors. The qualitative component included semi-structured interviews with CATI recipients, non-recipients, and implementers to assess CATI implementation. A total of 399 participants were enrolled in the quantitative evaluation conducted within 1 month of CATI delivery. For the qualitative evaluation, 41 semi-structured interviews were conducted, 30 with individuals in CATI areas (recipients and non-recipients) and 11 with CATI implementers. Handwashing with soap was low among both CATI and control area participants (1% vs. 2%, p = 0.89). Significantly more CATI area households (75%) had chlorine tablets present compared to control area households (0%) (p < 0.0001); however, the percentage of households with stored water free chlorine concentrations > 0.2 mg/L was low for both CATI and control area households (11% vs. 6%, p = 0.45). Implementers reported an insufficient supply of soap for distribution to recipients and mistrust in the community of their activities. CATI recipients demonstrated low knowledge of the correct preparation and use of chlorine for water treatment. Recipients also indicated a need for CATI implementers to engage community leaders. As CATIs are part of cholera control plans in many cholera-endemic countries, it is important to evaluate existing programs and develop evidence-based approaches to deliver CATIs that are both tailored to the local context and engage affected communities to increase WASH behaviors to reduce the spread of cholera.
Special Issue: Examining the Impacts of Social Determinants on HIV Health and Prevention
Authors: Boomer K. B.
Published: December 2023

Assessing the impact of social determinants of health (SDoH) has become an important part of the holistic view of an individual’s health status [...]
Development of a Gene-Based Marker Set for Orange-Colored Watermelon Flesh with a High β-Carotene Content
Authors: Jin Bingkui , Jang Gaeun , Park Girim , Shahwar Durre , Shin Jagyeong , Kwon Gibeom , Kim Yongjae , Kim Hoytaek , Lee Oakjin , Park Younghoon
Published: December 2023

The fruit flesh of watermelons differs depending on the distinct carotenoid composition. Orange-colored flesh relates to the accumulation of β-carotene, which is beneficial to human health. Canary-yellow-fleshed OTO-DAH and orange-β-fleshed (orange-fleshed with high β-carotene) NB-DAH near-isogenic lines (NILs) were used to determine the genetic mechanism attributed to orange watermelon flesh. For genetic mapping, an F2 population was developed by crossing the two NILs. The segregation ratio of flesh color in the F2 population indicated that the orange-β flesh of the NB-DAH NIL was controlled by a single incompletely dominant gene. Through a comparative analysis of the whole-genome sequences of the parent lines and NILs, a major introgression region unique to the NB-DAH NIL was detected on Chr. 1; this was considered a candidate region for harboring genes that distinguish orange from canary-yellow and red flesh. Among the 13 genes involved in the carotenoid metabolic pathway in watermelons, only ClPSY1 (ClCG01G008470), which encodes phytoene synthase 1, was located within the introgression region. The genotyping of F2 plants using a cleaved amplified polymorphic sequence marker developed from a non-synonymous SNP in ClPSY1 revealed its relationship with orange-β flesh. The insights gained in this study can be applied to marker-assisted breeding for this desirable trait.
The Function of ASK1 in Sepsis and Stress-Induced Disorders
Authors: Kostyak John C. , McKenzie Steven E. , Naik Ulhas P.
Published: December 2023

Apoptosis signal-regulating kinase 1 (ASK1) is a serine-threonine kinase that is ubiquitously expressed in nucleated cells and is responsible for the activation of multiple mitogen-activated protein kinases (MAPK) to regulate cell stress. Activation of ASK1 via cellular stress leads to activation of downstream signaling components, activation of transcription factors, and proinflammatory cytokine production. ASK1 is also expressed in anucleate platelets and is a key player in platelet activation as it is important for signaling. Interestingly, the mechanism of ASK1 activation is cell type-dependent. In this review we will explore how ASK1 regulates a variety of cellular processes from innate immune function to thrombosis and hemostasis. We will discuss how ASK1 influences FcγRIIA-mediated platelet reactivity and how that reactivity drives platelet clearance. Furthermore, we will explore the role of ASK1 in thromboxane (TxA2) generation, which highlights differences in the way ASK1 functions in mouse and human platelets.
Comparative Transcriptomic Analyses for the Optimization of Thawing Regimes during Conventional Cryopreservation of Mature and Immature Human Testicular Tissue
Authors: Pei Cheng , Todorov Plamen , Cao Mengyang , Kong Qingduo , Isachenko Evgenia , Rahimi Gohar , Mallmann-Gottschalk Nina , Uribe Pamela , Sanchez Raul , Isachenko Volodimir
Published: December 2023

Cryopreservation of human testicular tissue, as a key element of anticancer therapy, includes the following stages: saturation with cryoprotectants, freezing, thawing, and removal of cryoprotectants. According to the point of view existing in “classical” cryobiology, the thawing mode is the most important consideration in the entire process of cryopreservation of any type of cells, including cells of testicular tissue. The existing postulate in cryobiology states that any frozen types of cells must be thawed as quickly as possible. The technologically maximum possible thawing temperature is 100 °C, which is used in our technology for the cryopreservation of testicular tissue. However, there are other points of view on the rate of cell thawing, according to how thawing should be carried out at physiological temperatures. In fact, there are morphological and functional differences between immature (from prepubertal patients) and mature testicular tissue. Accordingly, the question of the influence of thawing temperature on both types of tissues is relevant. The purpose of this study is to explore the transcriptomic differences of cryopreserved mature and immature testicular tissue subjected to different thawing methods by RNA sequencing. Collected and frozen testicular tissue samples were divided into four groups: quickly (in boiling water at 100 °C) thawed cryopreserved mature testicular tissue (group 1), slowly (by a physiological temperature of 37 °C) thawed mature testicular tissue (group 2), quickly thawed immature testicular tissue (group 3), and slowly thawed immature testicular tissue (group 4). Transcriptomic differences were assessed using differentially expressed genes (DEG), the Kyoto Encyclopedia of Genes and Genomes (KEGG), gene ontology (GO), and protein–protein interaction (PPI) analyses. No fundamental differences in the quality of cells of mature and immature testicular tissue after cryopreservation were found. Generally, thawing of mature and immature testicular tissue was more effective at 100 °C. The greatest difference in the intensity of gene expression was observed in ribosomes of cells thawed at 100 °C in comparison with cells thawed at 37 °C. In conclusion, an elevated speed of thawing is beneficial for frozen testicular tissue.
Evaluation of Immune Response to Mucosal Immunization with an Oral Probiotic-Based Vaccine in Mice: Potential for Prime-Boost Immunization against SARS-CoV-2
Authors: Leontieva Galina , Gupalova Tatiana , Desheva Yulia , Kramskaya Tatiana , Bormotova Elena , Koroleva Irina , Kopteva Olga , Suvorov Alexander
Published: December 2023

Following the conclusion of the COVID-19 pandemic, the persistent genetic variability in the virus and its ongoing circulation within the global population necessitate the enhancement of existing preventive vaccines and the development of novel ones. A while back, we engineered an orally administered probiotic-based vaccine, L3-SARS, by integrating a gene fragment that encodes the spike protein S of the SARS-CoV-2 virus into the genome of the probiotic strain E. faecium L3, inducing the expression of viral antigen on the surface of bacteria. Previous studies demonstrated the efficacy of this vaccine candidate in providing protection against the virus in Syrian hamsters. In this present study, utilizing laboratory mice, we assess the immune response subsequent to immunization via the gastrointestinal mucosa and discuss its potential as an initial phase in a two-stage vaccination strategy. Our findings indicate that the oral administration of L3-SARS elicits an adaptive immune response in mice. Pre-immunization with L3-SARS enhances and prolongs the humoral immune response following a single subcutaneous immunization with a recombinant S-protein analogous to the S-insert of the coronavirus in Enterococcus faecium L3.
Molecular and Genetic Advances in Small Cell Lung Cancer Landscape: From Homogeneity to Diversity
Authors: Zullo Lodovica , Dall’Olio Filippo Gustavo , Rossi Giovanni , Dellepiane Chiara , Barletta Giulia , Bennicelli Elisa , Ingaliso Marta , Tagliamento Marco , Genova Carlo
Published: December 2023

Small cell lung cancer (SCLC) has been historically considered a homogeneous disease and thus approached as a single entity when it comes to clinical studies design and new treatments developments. However, increasing knowledge in the genetic and molecular landscape of this disease challenges this concept, opening the possibility that different subtypes might show differential vulnerability to treatments. In this narrative review, we gather the most relevant advances in genetic and molecular characterization of SCLC, focusing on how these discoveries may be used to design the path for a personalized treatment approach. Indeed, we discuss the new classification based on differential protein expression, the prevalence and significance of oncogenic drivers (e.g., EGFR mutations and ALK rearrangements) in SCLC, the genetic characteristics of SCLC in patients with no smoking history, and the existing evidence supporting the use of liquid biopsy for capturing the heterogeneity of the disease. We use the keywords “small cell lung cancer”, “SCLC”, “EGFR”, “ALK”, “histological transformation”, and “transcriptional factors” to identify original research manuscripts, clinical trials, case reports, and case series from PubMed.
Separation of Antioxidants from Trace Fraction of Ribes himalense via Chromatographic Strategy and Their Antioxidant Activity Supported with Molecular Simulations
Authors: Liu Youyi , Liu Chuang , Lei Yuqing , Guo Jingrou , Chen Xingyi , Wu Minchen
Published: December 2023

Antioxidants from natural sources have long been of interest to researchers. In this paper, taking the traditional Tibetan medicine Ribes himalense as an example, an integrated approach was used to identify and isolate its chemical composition with free-radical-scavenging properties from its ethanol extract. First, the ethanol extract of Ribes himalense was pretreated using polyamide medium-pressure liquid chromatography (polyamide-MPLC), and the target fraction (Fr4) was obtained. Then, a combined HPLC mode was utilized to purify antioxidants in Fr4 under the guidance of an online HPLC-1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH) activity screening system. Finally, three antioxidants (3-caffeoylquinic acid methyl ester, rutin, and myricetin-3′-α-L-rhamnopyranoside) were isolated, and this is the first report of their presence in R. himalense. Further molecular docking studies showed that the antioxidants exhibited good binding with HO-1, Nrf2, and iNOS. In conclusion, this comprehensive approach is capable of extracting high-purity antioxidants from trace fractions of Ribes himalense and holds promise for future applications in the exploration of the chemical compositions and bioactivity of natural products.
Investigation of the Potential of Selected Food-Derived Antioxidants to Bind and Stabilise the Bioactive Blue Protein C-Phycocyanin from Cyanobacteria Spirulina
Authors: Gligorijević Nikola , Jovanović Zorana , Cvijetić Ilija , Šunderić Miloš , Veličković Luka , Katrlík Jaroslav , Holazová Alena , Nikolić Milan , Minić Simeon
Published: December 2023

Blue C-phycocyanin (C-PC), the major Spirulina protein with innumerable health-promoting benefits, is an attractive colourant and food supplement. A crucial obstacle to its more extensive use is its relatively low stability. This study aimed to screen various food-derived ligands for their ability to bind and stabilise C-PC, utilising spectroscopic techniques and molecular docking. Among twelve examined ligands, the protein fluorescence quenching revealed that only quercetin, coenzyme Q10 and resveratrol had a moderate affinity to C-PC (Ka of 2.2 to 3.7 × 105 M–1). Docking revealed these three ligands bind more strongly to the C-PC hexamer than the trimer, with the binding sites located at the interface of two (αβ)3 trimers. UV/VIS absorption spectroscopy demonstrated the changes in the C-PC absorption spectra in a complex with quercetin and resveratrol compared to the spectra of free protein and ligands. Selected ligands did not affect the secondary structure content, but they induced changes in the tertiary protein structure in the CD study. A fluorescence-based thermal stability assay demonstrated quercetin and coenzyme Q10 increased the C-PC melting point by nearly 5 °C. Our study identified food-derived ligands that interact with C-PC and improve its thermal stability, indicating their potential as stabilising agents for C-PC in the food industry.
The Role of TAM Receptors in Bone
Authors: Engelmann Janik , Ragipoglu Deniz , Ben-Batalla Isabel , Loges Sonja
Published: December 2023

The TAM (TYRO3, MERTK, and AXL) family of receptor tyrosine kinases are pleiotropic regulators of adult tissue homeostasis maintaining organ integrity and self-renewal. Disruption of their homeostatic balance fosters pathological conditions like autoinflammatory or degenerative diseases including rheumatoid arthritis, lupus erythematodes, or liver fibrosis. Moreover, TAM receptors exhibit prominent cell-transforming properties, promoting tumor progression, metastasis, and therapy resistance in various cancer entities. Emerging evidence shows that TAM receptors are involved in bone homeostasis by regulating osteoblastic bone formation and osteoclastic bone resorption. Therefore, TAM receptors emerge as new key players of the regulatory cytokine network of osteoblasts and osteoclasts and represent accessible targets for pharmacologic therapy for a broad set of different bone diseases, including primary and metastatic bone tumors, rheumatoid arthritis, or osteoporosis.