bluuebunny/crossref_metadata_2025 huggingface dataset:
MiRNA-205-5p Promotes Development of Hepatocellular Carcinoma Via Targeting DLC1
Authors: xu yan , Tang weiming , Lu guang , Ji yin , Huang siyi , Peng ying , Kong xiaoming , Huang Ting
Published: December 2022

Purpose: Researching biomarkers is of great significance for immunotherapy of hepatocellular carcinoma (HCC). The purpose is to research the level and specific function of miR-205-5p in HCC and analyzed related genes, hoping to lay a theoretical basis for HCC treatment.
Methods: The HCC tissues and controls were obtained from hepatocellular carcinoma patients. Human liver immortalized cells (THLE2) and four HCC lines (huh-7, HepG2, HCCLM3, SK-Hep-1) were used in vitro. MiR-205-5p inhibitor, mimic, sh-CD302 Molecule (sh-DCL1) and their corresponding controls were transfected. Starbase and dual-luciferase reporter assay were undertaken for predicting and verifying the binding of miR-205-5p and DLC1. CCK8, EdU, flow cytometry assay was processed to detect cell viability, proliferation and apoptosis. Wound healing and transwell experiment were undertaken to monitor metastasis capability.
Results: There was higher level of miR-205-5p in hepatocellular carcinoma samples. Knockdown of miR-205-5p inhibited the proliferation and improved apoptosis of huh-7 and HepG2 cells. Moreover, miR-205-5p-inhibitor decreased the migration and invasion of HCC cells. MiR-205-5p targeted and negatively adjusted DLC1. Interestingly, sh-DLC1 rescued the influence of miR-205-5p-inhibitor.
Conclusion: MiRNA-205-5p promoted the occurrence and development of HCC via targeting DLC1, which was researched as a potential target for HCC therapy.
Evaluation of Surface Water Quality through CCME-WQI and Multivariate Statistical Techniques
Authors: Akiner Muhammed Ernur , Chauhan Pankaj , Singh Sudhir Kumar
Published: December 2022

The Betwa River Basin, a subbasin of the Yamuna, is the oldest flowing water system in Central India. Factor analysis and principal component analysis (FA/PCA) were multivariate statistical techniques used to extract three and four varimax factors that explained 96.408 and 100.000 percent of the total variance in water quality, respectively. Cluster analysis (CA) categorizes observed items into distinct quality categories based on correlations between stations and years. Point industrial/sewage effluents, non-point runoff from arable land and erosion, and natural source pollution are all factors that contribute to the pollution of the Betwa River, a mineral component of the water. As a result, water quality is threatened or impaired, and conditions often depart from natural or desirable levels at Rajghat, Garrauli, Mohana, and Shahijina stations. According to the Canadian Council of Ministers of the Environment Water Quality Index (CCME-WQI), the water quality ranking at the Rajghat and Mohana stations corresponds to fair ecological status. However, the Garrauli and Shahijina stations' surface water has marginal water quality status. From 1985 to 2018, the Shahijina had the most considerable load of nutrients and organic matter, as established by the CCME-WQI and by comparing the water quality data. A thorough examination revealed a fluctuating trend in the Betwa River pollution, particularly at all stations. Results indicate that between 1985 and 2018, the only defense mechanism of the river was the auto purification mechanism, which is strongly influenced by the drought, point resource of pollution, and extreme meteorological events that probably cause these fluctuations.
Early Childhood Mortality and Associated Factors in Ethiopia: Mini Demographic and Health Survey 2019
Authors: Damete Dereje Derbew , Lucha Tadele Abate
Published: December 2022

Background: Early childhood mortality is a major problem in terms of demographics, health, and development and factors that determine the size and growth rate of a population, as well as its ages and genders, along with its spatial distribution. The aim of this study was to determine factors associated with Early childhood mortality among children aged 0–59 months.
Methods: The data for this study was extracted from Ethiopia mini demographic and health survey 2019. The study involved 5414 women with children aged 0–59 months in a weighted sample. The analysis was performed using STATA version 16 software. Using sampling weights for descriptive statistics and complex sample designs for inferential statistics, backward stepwise elimination was manually applied. Finally, statistical significance declared at the level of p-value < 0.05.
Result: The prevalence of early childhood mortality in Ethiopia was observed to be 5.8%. In multivariate analysis the household wealth index, children from the poorest-wealth index (AOR = 1.211, 95% CI: 0.716-2.047) had higher odds of early childhood mortality in comparison to children from the richer wealth index. The odds of early childhood mortality were thirteen times higher among children born with twins’ birth as compared to single birth, (AOR = 13.615, 95% CI: 6.399-28.965). The odds of early childhood mortality were lower among children delivered in a health facility as compared to home delivery (AOR = 0.936, 95% CI: 0.893-0.982).
The odds of early childhood mortality were lower among tube well sources of drinking water as compared to piped sources of drinking water (AOR = 0.7128, 95% CI: 0.322-1.599). The odds of early childhood mortality were higher among pit latrine-type toilet facilities as compared to flush-type toilet facilities (AOR = 2.056, 95% CI: 1.319-3.207).
Conclusion: The prevalence of early childhood mortality in Ethiopia shows high risk and it is significantly associated with household wealth index, type of birth, place of delivery, sources of drinking water, and types of toilet facilities. Increasing the coverage and access to early childhood education, governmental and non-governmental organizations and all concerned stakeholders should be prioritized as the major determinants of early childhood mortality.
Ripple Effect noted in 15Mon Star using amateur astrophotography equipment.
Authors: Askari Hasan
Published: December 2022

Data collected by Gaia spacecraft has shown the presence of ripple effect in the stars, noted at the outer edge of the Milky-Way. The ripple effect has never been reported to be visible using any ground-based amateur telescope. In this article, I present astronomical images showing ripple effects in Star 15Mon at NGC 2264.
Biomarkers of atopic dermatitis: a meta-analysis over the last decade
Authors: Lyu An-Qi , Lyu Hao-Lin , Wu Chen-Xi , Shan Shi-Jun
Published: December 2022

Objective: A large number of studies had been published over the past decade on the association between the severity of atopic dermatitis and biomarkers. Our purpose is to screen relevant studies and comprehensively evaluate correlations between biomarkers and disease severity, which is an update for the researches in past decade.
Methods: Three databases (PubMed, EMBASE and Cochrane) were searched systematically, and relevant studies were selected according to the inclusion and exclusion criteria.
Results: A total of 49 articles reporting 98 different biomarkers in 4148 patients were rigorously evaluated. Ten markers (serum levels of CCL17, IgE, CCL27, CCL22, IL-13, IL-18, lactate dehydrogenase (LDH), IL-17A, vitamin D, and eosinophils) were used in at least four studies and meta-analyzed.
Conclusions: CCL27, IL-18 and IL-17A were found to be the most reliable biomarkers in the study, with correlation coefficients of 0.62, 0.62 and 0.61, respectively. Other biomarkers that may be useful but require more research include CCL17, IL-13, IgE, LDH, Eosinophil and Vitamin D.
The effects of apelin on IGF1/FSH-induced steroidogenesis, proliferation, Bax expression, and total antioxidant capacity in granulosa cells of buffalo ovarian follicles
Authors: Shokrollahi Borhan , Zheng Hai-Ying , Li Ling-Yu , Tang Li-Ping , Ma Xiao-Ya , Lu Xing-Rong , Duan An-Qin , Huang Chen-Xi , Xu Yuan-Yuan , Shang Jiang-Hua
Published: December 2022

Apelin (APLN) was believed to be an adipokine secreted from adipose tissue. However, studies demonstrate that it is a pleiotropic peptide and has several effects on the female reproductive system. In this study, the effect of different doses of IGF1 and FSH in the presence of APLN-13 on the production of estradiol and progesterone was evaluated in the follicular granulosa cells of buffalo ovaries, in addition, the effects of different doses of APLN isoforms (APLN-13 and APLN-17) on proliferation, the expression of Bax protein and total antioxidant capacity activity of the same cells were investigated. Granulosa cells of buffalo ovaries were cultured in the presence of different doses of IGF1 and FSH with or without APLN-13 (10− 9M) to evaluate its effect on the secretion of estradiol and progesterone that tested by ELISA assay. WST-1 method was used to survey the effect of APLN on granulosa cell proliferation and cytotoxicity. In addition, the antioxidant capacity of the cells in the presence of APLN was assessed using FRAP method. mRNA and Bax protein levels were measured in granulosa cells treated with APLN using real-time PCR and western blot techniques. APLN-13 stimulated the effect of IGF1 on the production of estradiol and progesterone, and the progesterone production levels were affected by APLN-13 dose-dependently. However, it did not significantly stimulate the effect of FSH on the secretion of estradiol or progesterone. APLN-13 (all doses) and − 17 (10− 8 and 10− 9 M) improved the proliferation of granulosa cells. Moreover, preincubation of the cells for an hour by APLN receptor antagonist (ML221, 10 µM) did not significantly affect the proliferation of cells. Neither APLN-13 nor APLN-17 were not cytotoxic for the cells compared to the control treatment. APLN-13 at the doses of 10− 6 and 10− 8 M substantially up and down-regulated Bax protein expression; however, such effects were not observed when the cells were preincubated with ML221. In addition, APLN-17 did not influence the expression amount of Bax. Furthermore, both APLN-13 and − 17 improved the total antioxidant capacity of the ovarian granulosa cells, but such effects were not seen when the cells were preincubated with ML221. These findings indicate that APLN enhanced the IGF1 induced steroidogenesis and improved the cell proliferation and antioxidant capacity of follicular granulosa cells of buffalo ovaries; however, its effect on Bax expression was divergent.
Pharmacogenomic Study—A Pilot Study of the Effect of Pharmacogenomic Phenotypes on the Adequate Dosing of Verapamil for Migraine Prevention
Authors: CHEN YI-CHIEH , Wang Han , Mandrekar Jayawant , Robertson Carrie , Starling Amaal , Cutrer Fred , Chiang Chia-Chun
Published: December 2022

OBJECTIVE
Using individual pharmacogenomic phenotypes to investigate the factors affecting the efficacy and tolerability of verapamil for migraine prevention.
BACKGROUND
Verapamil has a wide range of dosing in headache disorders without reliable tools to predict the optimal doses for an individual.
METHODS
This is a retrospective chart review examining adults with existing pharmacogenomic reports at Mayo Clinic who had used verapamil for migraine. Effects of six cytochrome P450 phenotypes on the minimum effective and maximum tolerable verapamil doses for migraine prevention were assessed.
RESULTS
The mean minimum effective and maximum tolerable doses for the 33 patients in final analysis were 178.2(20–320) mg and 227.9(20–480) mg. A variety of CYP2C9, CYP2D6, and CYP3A5 phenotypes were found, without significant association with the verapamil doses after adjusting for age, sex, body mass index, and smoking status.
CONCLUSIONS
We demonstrated a wide range of effective and tolerable verapamil doses used for migraine in a cohort with various pharmacogenomic phenotypes.
Biosorption of Pb (II) And Ni (II) By Protonated Industrial Waste Yeast Biomass
Authors: Yeneneh Anteneh Mesfin , Kaba Tassisa , Jafary Tahereh , Balushi Khadija Al , Mahrouqi Faisal Al , Adewole Jimoh , Kiamahalleh Meisam Valizadeh , Firouzie Amin
Published: December 2022

This study investigated the biosorption performance of waste yeast biomass derived from brewery for the removal of lead and nickel. Biosorption using low cost waste biomass is known to be an efficient alternative to the conventional technologies to remove heavy metals and organics from industrial effluents. In this study dried ground and protonated yeast used to investigate the removal of Lead (II) and Nickel (II) under the influence several parameters including pH, contact time, presence of co-ions, metal, and sorbent dose. The equilibrium and kinetics of the sorption process and heavy metal recovery tests were also carried out. Significant proportion of the heavy metal ion was sorbed during 5 to 10 minutes of contacting and equilibrium was reached within 60 minutes where the optimum sorption time was observed to be at 30min. Langmuir and Freundlich adsorption models were used in the equilibrium study to fit the equilibrium data after 24 hours of contacting. The kinetics of the metal uptake process was also investigated for varying metal dose in the range of 10-200mg/l and yeast dose in the range of 0.5–4 g/l. qe shows an increasing trend for increasing metals dose and is inversely related to yeast dose, similarly, k and h also showed increasing trend for increasing metal dose. Furthermore, test for ion interference effect or co-ion tests were conducted where sorption of lead decreased from 576 mg/g to 444.5 mg/g when nickel concentration was increased from 50 mg/l to 700 mg/l. Based on recovery tests, the recoverability of lead (80%) was significantly higher than that of nickel (38%). The study has proven that waste yeast industrial biomass can be used for large scale sorption applications in house or in other heavy metal waste generating industries.
High Prevalence of Anxiety Symptoms in Venezuelan Adults during the Beginning of the Venezuelan Crisis. Data from a National Representative Sample. The EVESCAM Study
Authors: González-Rivas Juan P. , Iglesias-Fortes Rocio , De Oliveira-Gomes Diana , Infante-García Maria M. , Durán Maritza , Ugel Eunice , Marulanda María Inés , Novotny Sebastian , Stokin Gorazd B , Mechanick Jeffrey I. , Nieto-Martìnez Ramfis
Published: December 2022

Venezuelans have been living in a humanitarian crisis since 2014 and the effect of this adverse environment on the mental of the population is unknown. This article aims to determinate the prevalence of anxiety and depressive symptoms of adults and factors related during the beginning of the humanitarian crisis of Venezuela. Anxiety and depressive symptoms were determined using the Hospital Anxiety and Depression Scale (HADS). 3,241 adults were evaluated with mean age of 41.1 ± 15.7 years. Anxiety prevalence was 14.7% (women 19.3% and men 9.5%; p < 0.001) and depression prevalence was 3.1% (women 4.0% and men 2.2%; p < 0.001). Independently of age and gender, anxiety was associated with hypertension and tobacco use, and depressive symptoms was associated with smokeless tobacco use, and sedentary lifestyle. Compared to subjects with high/middle socioeconomic status (SES), those with poor and extremely poor SES were more likely to have anxiety, and depression.
Green Synthesis and Catalytic Activity Assessment of Bespoke Nano- Catalyst for Eco-Friendly Green Propellant Systems based on Hydrogen Peroxide
Authors: Elbasuney Sherif , Attwa Mohamed , Deif A. , ElGamal M. , Fayoud Ahmed , Gobara M.
Published: December 2022

Much effort has been devoted to replace pollutant, toxic, cancerogenic hydrazine-based propellants. Hydrogen peroxide could offer promising characteristics as well as high density specific impulse. Effective catalysts with high durability are required to initiate H2O2decompositions, or to inherit hypergolic nature with different fuels. This study reports on the novel green synthesis of silver nanoparticles (18 nm) via hydrogen evolved by water electrolysis. MnO2(20 nm) was developed in a sustainable manner via green hydrothermal processing. High crystalline, mono-dispersed particles were developed. Catalytic activity was assessed via precise measurements of liquid temperature profile (LTP) to reach the boiling point of hydrogen peroxide. Whereas silver demonstrated LTP peak at 20 seconds; MnO2experienced LTP of 40 seconds. Catalyst survivability was recorded via precise measurement of life time mass loss rate upon catalyst addition to hydrogen peroxide. While silver nanocatalyst demonstrated high performance at the reaction start; silver was found to be poisoned with crystalline phase change to silver oxide within 20 seconds. On the other hand, manganese oxide experienced high durable catalytic action. Consequently, MnO2could be candidate for H2O2monopropellant thrusters as catalyst bed. On the other hand, silver nanoparticles could be candidate for a single use in bipropellant to inherit hypergolicity.
Investment-grade corporate bond as the top-performing asset class and its safe-haven role during the COVID-19 pandemic
Authors: Wang Qiyu
Published: December 2022

In this paper, we compare asset classes performance quantitatively, including worldwide stock indices, bonds of different grades, gold, energy and foreign exchange. Bonds, especially investment-grade corporate bonds (Moody’s DBAA), performed the best among all the asset classes. Prices plunged significantly in Phase I, from COVID-19 start to March 2020and re-bounced after CARES (Coronavirus Aid, Relief, and Economic Security). We thus analyze the quantitative results through subperiods including pre-COVID-19 subperiod, Phase I, Phase II and time after lockdown and also compare to situations in the subprime crisis. The COVID-19 period asset correlations among asset classes increased than the usual. Since the means and variances of asset returns are not stationary, dynamic conditional correlation (DCC) model computes correlations between DBAA and other assets. Test results of the Phase I, Phase II, or extreme quantiles dummy variables’ impacts on the DCC time series confirm DBAAs safe-haven role.
Association of the Renin-Angiotensin System Components in Human Follicular Fluid with Age, Ovarian Function and IVF Outcome: A Cross-Sectional Study
Authors: Wei Lun , Bo Le , Zhou Anwen , Luo Chao , Wu Zhinan , Lu Xuanping , Zhan Lei , Gao Shasha , Qian Fei , Mao Caiping
Published: December 2022

Background
Previous studies have found that there is the local renin-angiotensin system (RAS) in human ovary, and it independently plays a regulatory role in ovarian function. Age-related reproductive aging is an inevitable process. To explore the relationship between RAS components in human follicular fluid (hFF) and age, ovarian function, so as to help clinical evaluation of oocyte quality and prediction of in vitro fertilization (IVF) outcome.
Methods
hFF of 139 women who received IVF simply because of their husband's factors from January 2021 to February 2022. The levels of RAS components in hFF were measured, including Renin, ACE, ACE2, AngⅡ and Ang(1–7). The correlation between age and RAS in hFF was analyzed by simple linear regression, and multivariate linear regression was used to further analyze the correlation between the RAS and IVF outcome.
Results
A total of 139 samples of analysable hFF were obtained in this study. It was found that there was a linear negative correlation between age and renin, ACE, AngⅡ, ACE/ACE2, AngⅡ/Ang(1–7) (Pearson's r < 0, P < 0.05), while the linear relationship with ACE2 and Ang (1–7) was not significant (P > 0.05). It was found that bFSH and bLH were correlated with age, ACE-AngⅡ-AT1/2R axis, ACE2-Ang(1–7)-MAS axis (P < 0.05), AFC was correlated with age, ACE2-Ang(1–7)-MAS axis (P < 0.05), and bAMH was only correlated with age (P < 0.01). There was no correlation between RAS and bE2, bP, bPRL, bT (P > 0.05). It was found that the number of oocyte retrieval and MⅡ maturation rate were correlated with age, renin, ACE-AngⅡ-AT1/2R axis and ACE2-Ang(1–7)-MAS axis (P < 0.05), and the rate of 2PN embryos, transplantable embryos, high-quality embryos were only correlated with age (P < 0.01).
Conclusions
①The influence of age on ACE-AngⅡ-AT1/2R axis was more significant than that on ACE2-Ang(1–7)-MAS axis in hFF; ②Provides evidence that gonadotropins mediate RAS activation of local ovarian follicles; ③It is still impossible to prove the effect of RAS on steroid hormone production, at least in ovarian follicles RAS cannot affect steroid hormones in the systemic circulation; ④Age and the level of RAS components in hFF have certain clinical value in predicting the number of oocyte retrieval and the rate of MⅡ maturation.
Steroid Receptor Coactivator 1 Promotes Human Hepatocellu-lar Carcinoma invasiveness Through Enhancing MMP-9
Authors: Tong Zhangwei , Zhang Yong , Guo Peng , Wang Wei , Chen Qiang , Jin Jing , Liu Shixiao , Yu Chundong , Mo Pingli , Zhang Lei , Huang Junli
Published: December 2022

Backgroud: SRC-1 works as a transcriptional coactivator for steroid receptors and other transcrip-tional factors. SRC-1 is shown to play oncogenic roles in many cancers, like breast cancer and prostate cancer. Our lab anteriorly accounted that SRC-1 is highly expressed in human HCC spec-imens. SRC-1 accelerates HCC progression via enhancing Wnt/beta-catenin signaling. However, the role of SRC-1 in HCC metastasis is unknown.
Methods: RNA inteference was used to knockdown the expression of SRC-1, and the protein level was detected via Western blot assay. Matrigel invasion assay was performed for assessment of HCC cell metastasis. MMP9 expression was detected via Zymography. Luciferase assays were performed to detect MMP-9 promoter activity.
Results: In this study, we report that SRC-1 promotes HCC metastasis through enhancing MMP-9 expression. Knockdown of SRC-1 decreased HCC cell metastasis in vitro and in vivo by inhibiting the expression of MMP-9. SRC-1 mRNA level is found to positively correlated with MMP-9 mRNA level in a limited number cohort of HCC specimens and a larger number cohort of HCC specimen from GEO database. SRC-1 functions as a coactiva-tor for NF-κB and AP-1 to regulate MMP-9 promoter activity in HCC cells. Higher SRC-1 and MMP-9 expression correlates with a worse overall survival in HCC patients. Bufalin treatment, which can inhibit SRC-1 expression, can significantly decreased MMP-9 expression and inhibit HCC metastasis both in vitro and in vivo.
Conclusion: Our results demonstrated that SRC-1 is a crucial modulator for HCC metastasis and offered a potential target for HCC therapy.
Calcium-Mediated DAD in Membrane Potentials and Triggered Activity in Atrial Myocytes of ETV1 f/f MyHC Cre/+ Mice
Authors: Fang Li-Hua , Cheng Xian-Lu , Chen Qian , Li Xiao-Qian , Zou Tian , Chen Jian-Quan , Xiang Guo-Jian , Li Yang , Zhang Jian-Cheng , Zhu Peng-Li
Published: December 2022

The E-twenty-six variant 1 (ETV1) -dependent transcriptome played an important role in atrial electrical and structural remodeling and the occurrence of atrial fibrillation，but the underlying mechenism of ETV1 in atrial fibrillation is unclear. In this study, cardiomyocyte-specific ETV1 knockout (ETV1f/fMyHCCre/+, ETV1-CKO) mice were constructed to observe the susceptibility to AF and the underlying mechanism in AF associated with ETV1-CKO mice. AF susceptibility was examined by intraesophageal burst pacing，induction of AF was increased obviously in ETV1-CKO mice than WT mice. Electrophysiology experiments indicated shortened APD50 and APD90, increased incidence of DADs, decreased density of ICa,L in ETV1-CKO mice. There was no difference in V1/2 INACT and V1/2 ACT, but a significantly longer duration of the recovery time after inactivation in the ETV1-CKO mice. The recording of intracellular Ca2+ showed that there was significantly increased in the frequency of calcium spark, Ca2+ transient amplitude, and proportion of SCaEs in ETV1-CKO mice. Reduction of Cav1.2 rather than NCX1 and SERCA2a, increase RyR2, p-RyR2 and CaMKII was reflected in ETV1-CKO group. This study demonstrates that the increase in calcium spark and SCaEs conresponding to Ca2+ transient amplitude that may trigger DAD in membrance potential in ETV1-CKO mice, thereby increasing the risk of AF.
Comparison of confidence among graduate teaching assistants before and after training
Authors: Peng Hexiang , Wu Yiqun , Zhou Ren , Chen Xi , Wang Mengying , Ren Tao , Yu Chihui , Wu Tao
Published: December 2022

Background: Teaching assistants (TAs) play an important role in pedagogical practices, and teaching assistant (TA) training has become a critical measure to promote teaching quality and interaction. TAs’ confidence might significantly increase the performance of TAs’ work. However, little evidence paid attention to the TAs’ confidence changes after TA training.
Methods: A self-control quasi-experiment was conducted to explore the TAs’ confidence changes before and after TA training. A qualified questionnaire was used to investigate the confidence of the graduate teaching assistants before and after the TA training.
Results: A total of 372 TAs from school of basic medicine (N=173), school of pharmacy (N=112), school of public Health (N=69) and other schools (N=18) submitted complete questionnaires. They had a median total confidence score of 88 and 85 before and after the TA training. The total TA confidence score decreased after TA training (P<0.001). For imparting new knowledge accurately to students, those who were disinterested in becoming a college teacher were associated with an increased confidence compared with neutral TAs (β:6.89, 95%CI: 0.94,12.83).
Conclusion: TA confidence decreased after TA training. Therefore, in addition to teaching skills training, it is necessary to establish and strengthen TAs’ confidence when designing TA training programs.
WormTensor: a clustering method for time-series whole-brain activity data from C. elegans
Authors: Tsuyuzaki Koki , Yamamoto Kentaro , Toyoshima Yu , Sato Hirofumi , Kanamori Manami , Teramoro Takayuki , Ishihara Takeshi , Iino Yuichi , Nikaido Itoshi
Published: December 2022

Background
In the field of neuroscience, neural modules and circuits that control biological functions have been found throughout entire neural networks. Correlations in neural activity can be used to identify such neural modules. Recent technological advances enable us to measure whole-brain neural activity with single-cell resolution in several species including Caenorhabditis elegans. Because current neural activity data in C. elegans contain many missing data points, it is necessary to merge results from as many animals as possible to obtain more reliable functional modules.
Results
In this work, we developed a new time-series clustering method, WormTensor, to identify functional modules using whole-brain activity data from C. elegans. WormTensor uses a distance measure, modified shape-based distance to account for the time delay and the mutual inhibition of cell-cell interactions and applies the tensor decomposition algorithm multi-view clustering based on matrix integration using the higher orthogonal iteration of tensors (HOOI) algorithm (MC-MI-HOOI), which can estimate both the weight to account for the reliability of data from each animal and the clusters that are common across animals.
Conclusions
We applied the method to 24 individual C. elegans and successfully found some known functional modules. Compared with a widely used consensus clustering method to aggregate multiple clustering results, WormTensor showed higher silhouette coefficients. Our simulation also showed that WormTensor is robust to contamination from noisy data. WormTensor is freely available as an R/CRAN package https://cran.r-project.org/web/packages/WormTensor.
Unconditional Stability and Fourth-Order Convergence Of A Two-Step Time Split Explicit/Implicit Scheme For Two-Dimensional Nonlinear Unsteady Convection-Diffusion-Reaction Equation With Variable Coefficients
Authors: Ngondiep Eric , Tedjani Ali H.
Published: December 2022

This paper deals with an efficient two-step time split explicit/implicit scheme applied to a two-dimensional nonlinear unsteady convection-diffusion-reaction equation with variable coefficients. The computational cost of the new algorithm at each time level is equivalent to solving a pentadiagonal matrix equation with strictly dominant diagonal elements. Such a bandwidth matrix can be easily inverted using the Gaussian Decomposition and the corresponding linear system should be solved by the back substitution method. The proposed approach is unconditionally stable, temporal second-order accuracy and fourth-order convergence in space. These results suggest that the developed technique is faster and more efficient than a large class of numerical methods studied in the literature for the considered initial-boundary value problem. Numerical experiments are carried out to confirm the theoretical analysis and to demonstrate the performance of the constructed numerical scheme.
Medical physiological perspective to biochemical assays and GC-MS results of corn tassel
Authors: YAMAN Burak
Published: December 2022

Corn tassel (Zea may L.) is rich in phenolic compounds including flavonoids and anthocyanins. The aim of this study is to consider the results of the contents of phenolics (TPC), flavonoids (TFC), flavonol (TF), anthocyanins (TAC), alfa-amylase inhibitory activity, and antioxidant activity including FRAP and metal chelating capabilities (MCC) as potential Antiviral and anti-Rheumatoid arthritis. Significantly high levels of antioxidant capacity, total flavonol and alfa-amylase inhibition were found in ethanolic extracts of corn tassels. It was found that their concentrations are TPC= 40 mg GA/g, TFC= 13 mg QE/g, TF= 45 mg R/g, and TAC= 8 mg cyanidin-3-glucoside/g based on dry extract. Additionally, the extracts showed relatively higher antioxidant activities due to metal chelating capabilities (MCC) were found to be 217 mg Fe2+/g dry extract. From the GC-MS analysis, corn tassel was found to be good source of arctigenin that has antiviral and anti- rheumatic properties. Further, the extracts of corn tassels showed significantly higher α-amylase inhibitory activity up to 90 %. Thus, it was concluded that extracts of corn tassels may be considered as pharmacological potential in rheumatoid and antiviral treatment.
Parsing Syntaxis on Language Acquisition of 3-4-Year-Old Children of Playgroup Darussalam Banyuwangi
Authors: Nauvalia Nurin , Salikin Hairus
Published: December 2022

The language acquisition of each child is different. Some children are perfect and good at language, but other children still have difficulties in language. This causes the other person also have difficulty understanding what they are saying. The purpose of this study was to determine the acquisition of language in children aged 3-4 years and to know the Parsing Syntax of the language of children aged 3-4 years in the Darussalam Study Group Blokagung Banyuwangi. The research design used is descriptive qualitative. Data were collected by applying observation and interviews with the teacher and the students of Playgroup of Darussalam. The data were analyzed using the Interactive model of Miles, Huberman, and Saldana. The results showed that language acquisition was obtained from the family environment, social environment, and school environment. The students of the Darussalam Blokagung Banyuwangi Playgroup have basic language acquisition. They tend to imitate or learn from the surrounding environment, family, school, or social environments.
Video-Based Tasks in Strengthening Speaking Skills of EFL College Students
Authors: Menggo Sebastianus , Basir Agnetis , Halum Yustus Sentus
Published: December 2022

All teachers, including speaking instructors, should be able to select the proper interactive media for students' speaking practice. Video-assisted learning tasks in the speaking class have not been explored extensively, despite the reality that video-based tasks are an appropriate choice for students to enhance their speaking ability. This study sought to examine the Effect of video-based tasks on students' speaking skills. In this study, a quasi-experimental design was employed, and the speaking scoring rubric served as the research instrument. Both the experimental group and the control group were comprised of participants. Researchers gave pre-and post-tests to identify the impact of the video-based tasks technique on students' speaking skills. Using a simple random sampling technique, 56 students from the English study program in the first grade at Universitas Katolik Indonesia Santu Paulus Ruteng comprised the research sample; then, the data were analyzed by using a t-test with the assistance of the SPSS 24.0 program. The data analysis of the average student score in the experimental and control groups revealed that the experimental group's average score (80,29) was higher than the control group's average score (67,57). This result demonstrates the significant effects of video-based tasks in teaching speaking. The researchers concluded that video-based tasks were helpful for teaching speaking skills. Moreover, video-based tasks significantly impact students' ICT skills, learning independence, and self-evaluation development. The logical implication of this study's findings is that EFL teachers should use videos to teach English, particularly speaking skills, because it has been demonstrated to have a favorable effect on the respondents' speaking ability.
“Holy Moly!” Positive and Negative Connotations of Swear Words in A Popular Western Animated Movie
Authors: Muthalib Kismullah Abdul , Fata Ika Apriani , Nuzula Zahra
Published: December 2022

The use of swear words is common and even popular in movies for adult viewers. However, misunderstanding and ignorance of the intended viewers can cause younger viewers to be exposed to movies with various profanities. The Simpsons, an animated movie, is a good example of this phenomenon. This animated movie is known to contain obscene language expressions which can be mistakenly assumed to be intended for younger viewers. This study sought to identify the kinds and purposes of expletives used in The Simpsons. The current study used Hughes’ framework to analyze the types of swear words, and Andersson and Trudgill's framework was used to analyze their functions. Data were gathered from seven episodes drawn from different seasons of the animated series. Of 102 swear words found in the dialogues of the episodes, seven types of swear words were identified, including anatomical insult, excrement, sexual intercourse, personal background, mental illness, animal term, and religious oath. Additionally, four swearing meanings and functions were discovered abusive, auxiliary, expletive, and humorous. Therefore, it is expected that general readers will express themselves more thoughtfully, be able to identify the target audience for movies, and make more informed decisions when choosing movies for younger audiences.
Speech Function in Teaching Speaking Skill: Evaluation, Analysis, and Recommendation
Authors: Taridi Muhamad , Fitria Wahyuni , Supriusman Supriusman , Deska Revini
Published: December 2022

This study aims to assess and analyse the sorts of speech functions used by teachers in the tenth grade at one vocational high school in Jambi, Indonesia, and to identify the most prevalent kind. The study was carried out using descriptive qualitative research and descriptive analysis design. In this study, data were triangulated through observation and documentation. The study's targets were English teachers, and the transcribed data was divided into three categories of language functions. Holmes' concept is applied to classify speech functions. There are three types of utterance functions: expressive, imperative, and referential. The results showed that out of 60 speech functions, the directive function was applied by instructors the most frequently, accounting for 31 of them (51.66%). Directive one is the main reason, as it delivers interaction by directing and requesting the student. The study recommends that there are still many types of speech functions, but alternatively, directives are more prominent. Other researchers can gather data from many sources, including Instagram's English-language account, an English newspaper or magazine, and many more. The current study encourages English as a Foreign Language (EFL) teachers to complete a task and determine an assignment for future research. Given the implications of directive functions for English teachers in teaching speaking skills and the ability to inquire about something and command and request learners to perform something.
Understanding Frame on the Mindset of Orang Rimba Related to Pandemic Words
Authors: Rozelin Diana
Published: December 2022

Understanding Frame on the Mindset of Orang Rimba Related to Pandemic Words Diana RozelinPostgraduate SchoolUIN Sulthan Thaha Saifuddin  Jambi, Indonesiae-mail: dianarozelin@uinjambi.ac.id Abstract:During the Covid-19 pandemic, some terms appear to represent the coronavirus. For some people, Covid-19 terms were familiar, but not for others, such as Orang Rimba (OR). Some terms were weird and new because it was pronounced in the English language, such as 'social distancing', 'rapid test', and 'lockdown'. Meanwhile, the role of the government, in this case, BKSDA was very important to stop the spread of this virus within the OR community. This article not only talked about language but also the implementation of health. The purposes of this research were: 1) to find out the government's role through BKSDA in educating OR about Covid-19 terms and its preventing actions; 2) to find out the perception of OR toward the words of Covid-19. This research was conducted qualitatively. The technique for collecting the data was observation, interview, documentation, and recording. Meanwhile, for analyzing the data used descriptive techniques. The results were: 1) BKSDA office visited each OR group and gave directions and examples on how to wash hands, wear masks, keep their distance, and keep the environment clean. The suitable methods in teaching new things to OR are exemplary and habituation. 2)  from 15 vocabulary words related to the Covid-19 pandemic, OR knew only 5 words. It was a mask, virus, washing hands, hand sanitizer, and corona. The word mask, according to OR, was a fabric for covering the nose and mouth. The meaning of the virus and corona were evil animals. The word washing hand means besuh tangon/ cuci tangon.
Men’s Language and Masculine Identity Construction in Sexist Jokes
Authors: Nursanti Emi
Published: December 2022

The power imbalance between men and women in society is reflected in their language use and it may be reinforced in sexist jokes. This is a sociolinguistic study on men’s language in sexist jokes on the Internet. This study is aimed at investigating men’s linguistic features and the masculine identities represented by those features. This is a descriptive qualitative study with complementary quantitative analysis. The theories of Tannen’s men language (1990) and Kiesling’s masculine identities (2007) were used as references to analyze the data. The results show that men’s identity of competing for solidarity embodied in discourses of insults, teasing, and joking is the most dominant identity represented in sexist jokes. This is in line with the main intention of sexist humor to laugh at women’s inferiority. Meanwhile, men’s dominance realized in report talk becomes the second most prevalent identity. The identities of politeness oaf, indicated by direct command, and freedom, shown by swearing and taboo words, are not influential as both do not contribute to the making of humorous expressions. These imply that context and communication purposes motivate speakers to use gender-related features. Reflecting that most of the masculine identities and the language features in sexist jokes represent men’s superiority, this kind of humor can be a way to maintain gender asymmetry in society. Besides, although humor leads to laughter and is considered healthy, sexist humor is dangerous as the expressions subordinate women. Therefore, being critical when reading the jokes and trying to avoid such jokes in communication is suggested.
Patriarchal Hegemony in the Novel “Women at Point Zero” by Nawal El Saadawi
Authors: Istifadah Istifadah , Rohmana Wahyu Indah Mala
Published: December 2022

This study aims to describe and analyze the forms of patriarchal hegemony in the novel Woman at Point Zero by Nawal El Saadawi. The patriarchal hegemony is then correlated with the teachings of Islam, which are often seen as supporting the domination of men. It is a literary criticism that analyzes and interprets literary work. Sylvia Walby’s six structures of patriarchy theory and Gramsci’s theory were used to analyze the data. The data were taken from the written work of Woman at Point Zero English Translation published in 2007. Data were taken by identifying, classifying, and analyzing the data by decoding them with the theories. The result of this study shows the variety of gender injustice and biases in the form of physical, verbal, sexual abuse, marginalization, and exploitation of women in the work. The implication of the research is to provide concrete evidence that Islamic teaching never provokes man domination and patriarchal practices over the woman.
Boosting Students’ Speaking skill using Ted Talks YouTube Videos: Teacher Talk Theory
Authors: Karimah Imroatul , Setiawan Slamet , Munir Ahmad
Published: December 2022

Even though the importance of teaching English speaking using TED Talks to improve students’ speaking skill has been acknowledged, however, far too little attention has been paid to investigate students’ perceptions on the use of TED Talks dealing with teacher talk categories such as dealing with feelings, praising and encouraging, making joke, as well as giving direction and lecturing. This study aimed to investigate the concordance of the students’ speech ability development and the students’ interest and motivation towards the use of TED Talks to boost the students’ speaking skill. This present used both quantitative and qualitative design. The samples of the students were 27 students from grade 7E in MTsN Surabaya. For the quantitative data, the data was collected using pre-test and post-test. However, for the qualitative data, the data was gathered using interview protocol. The data was analyzed using descriptive analysis. The findings of this study found that the students improve their English speaking skill especially the pronunciation and the fluency of delivering English speech in front of their friends after the treatment using the TED Talks videos. Regarding the students’ perceptions of the use TED Talks to support teacher talks, most of the students agreed that TED Talks videos can support teacher talks related to the category of dealing with feelings, praising and encouraging, making jokes and giving direction as well as lecturing.
Influence of state policy components on the rate of violence and crime against human life and health
Authors: Aliyeva Zamina
Published: December 2022

The share of crimes against human life and health on average is up to 10% worldwide, and losses are estimated from 0.3 to 3% of GDP. This study examines the dependence of the rate of violence and crime against human life and health on the state policy elements in the example of transitioning and developing countries. The crime index, the share of people reporting crime, the rate of violence or vandalism in the area, and the number of intentional homicide offenses in the largest cities were used as parameters characterizing the rate of violence and crime against human life and health. All parameters were divided into institutional, social, and economic. The dependence between the indicators was studied using fixed-effects and random-effects models; a grouping of countries according to the nature of this dependence employed the iterative separation method of k-means and tree clustering. Based on the results, it is justified that institutional and economic (highest GDP and real minimum wages) components significantly influence the level of violence and crime against human life and health. For example, the average value of the crime index for the fourth cluster is 29.98 compared to 54.09 for the first cluster. At the same time, strengthening responsibility for committed crimes has a more negligible impact on the crime level than increasing the material well-being of the population and supporting its vulnerable segments.
Hydroinformatics as a ‘game changer’ in the water business
Authors: Zeman E. , Metelka T. , Ingeduld P. , Shipton S.
Published: December 2022

This chapter presents the field of hydroinformatics as a game-changer in the water sector business. It reflects on Mike Abbott's original ideas and evaluates their impact from the perspective of water business owners, water consultants, clients, and communities. Mike Abbott promoted a novel concept, combining theoretical and practical aspects of modelling, towards ‘industrial modelling systems’. The chapter traces the development of this concept, from its early adoption by government-funded research institutes engaged in scientific support to hydraulic engineering, through establishment of market for water-modelling software products, to its broad adoption by consultants. Mike Abbott also saw a significant opportunity for hydroinformatics in the developing world. New policies and businesses were easier to implement in countries without a mature market of ideas resisting a change. Many of his followers were eager to explore business paths, which were much faster and more agile than those in academic circles. Most large companies that are currently developing commercial applications and tools for water modelling matured during the so-called ‘third modelling generation’, when generic modelling tools started to be developed. Subsequently, the real growth was observed when the knowledge encapsulation and robustness of the applications expanded their use from specialists to a broad pool of water professionals and practitioners. The social impact of hydroinformatics was significant as it spread through a wide range of organisations impacting decision making, education, innovation and knowledge sharing. As modelling systems became a norm in the water business, the business itself has been transformed. Impacts of current technologies and new data sources on water modelling business are presented (such as the Cloud, High Performance Computing - HPC, virtualisation, internet of things - IoT, remote sensing, and others), and how they impact current needs of software producers and users. Diverse business models have emerged, which need to meet the future challenges of urban and environmental water management under challenges of climate change. With the large and continuously growing community of modelling experts and users, it can be argued that the Mike Abbott's vision of hydroinformatics has been well established.