bluuebunny/crossref_metadata_2025 huggingface dataset:
Woody Species Composition, Diversity, and Recovery Six Years after Wind Disturbance and Salvage Logging of a Southern Appalachian Forest
Authors: Oldfield Callie A. , Peterson Chris J.
Published: February 2019

Salvage logging after wind disturbance of a mixed conifer-hardwood forest results in sapling compositional changes but no changes to species diversity six years post-disturbance. Several conceptual frameworks allow for predictions of the effects of forest disturbances on composition, but fewer yield predictions of species diversity. Following compound disturbance, tree species diversity and composition is predicted to shift to early successional species. Because of the greater cumulative severity, diversity should be lower in areas experiencing windthrow + salvage logging than in similar sites experiencing windthrow alone. We examined the effects of wind disturbance and salvage logging on diversity parameters over six years. We hypothesized that the effects of salvage logging on diversity would be short-lived, but that species composition would be altered six years post-disturbance. Sampling plots were established in a mixed-hardwood forest in north Georgia, USA, after a 2011 EF3 tornado and surveyed in 2012 and 2017. Nineteen 20 × 20 m plots were surveyed (10 unsalvaged, 9 salvaged) for parameters including Shannon diversity, species richness, and composition. Ordinations were used to visualize tree and sapling species composition in salvage logged plots. We found that there was no significant difference in Shannon diversity between salvaged and unsalvaged plots before disturbance, <1 post-disturbance, or 6 years post-disturbance. The disturbances altered the tree and sapling species compositions, with salvaged plots having more mid-successional saplings but few true pioneer species. There appears to be an emerging pattern in the wind disturbance + salvaging literature which our study supports– salvaging does not affect tree species diversity but shifts species composition over time.
Identification and Analysis of microRNAs in the SAM and Leaves of Populus tomentosa
Authors: Cui Jiawen , Lu Weichao , Lu Zhaogeng , Ren Shixiong , Zhao Beibei , Wang Li , Teng Nianjun , Jin Biao
Published: February 2019

The shoot apical meristem (SAM) is a crucial tissue located at the tops of plants which can continually grow and differentiate to develop into all aboveground parts. SAM development is controlled by a series of complicated molecular regulation networks, among which microRNAs (miRNAs) and their target genes play key roles. However, little is known about these miRNAs in woody plants. In this study, we used small RNA (sRNA) sequencing to build four libraries derived from shoot tips and mature leaf tissues of Populus tomentosa, and identified 99 known miRNA families. In addition, 193 known miRNAs, including phytohormone-, developmental-, and cellular process-related miRNAs, showed significant differential expression. Interestingly, quantitative real-time reverse transcription polymerase chain reaction (PCR) analysis of miR172, miR164, and miR393 expression showed marked changes in expression patterns during the development of shoot tips. The target genes of these miRNAs were involved in the regulation of hormone responses and stem cell function. In particular, the miR172 target APETALA2 (AP2), involved in the maintenance of stem cells in the shoot apex, was expressed specifically during the initial active stage of development. These findings provide new insights into the regulatory mechanisms of miRNAs involved in SAM development and differentiation in tree species.
Infection Levels of the Microsporidium Larssoniella duplicati in Populations of the Invasive Bark Beetle Ips duplicatus: From Native to New Outbreak Areas
Authors: Zimová Soňa , Resnerová Karolina , Vanická Hana , Horák Jakub , Trombik Jiří , Kacprzyk Magdalena , Lindelöw Åke , Duduman Mihai-Leonard , Holuša Jaroslav
Published: February 2019

The microsporidium Larssoniella duplicati (Weiser, Holuša, Žižka, 2006) is a specific pathogen of the bark beetle Ips duplicatus (C.R. Sahlberg, 1836), which is a serious pest of Norway spruce (Picea abies (L.) H. Karst) in Europe. From 2011 to 2016, infection levels of L. duplicati and other pathogens in I. duplicatus populations were assessed along a gradient, ranging from areas in the north, where the beetle is native, to areas in the south, where the beetle has only recently invaded. The 21 study sites ranged in altitude from 229 to 1009 m a.s.l. We found that pathogen infection levels in I. duplicatus populations decreased from the native areas in the north to the new areas of beetle expansion in the south. We also found that pathogen level increased with altitude. The L. duplicati infection levels were not associated with the infection levels of other beetle natural enemies. The infection level decreased with the length of time of beetle establishment in an area. The infection level increased with the number of beetles trapped and dissected at a site.
Can Existing Estimates for Ecosystem Service Values Inform Forest Management?
Authors: Müller Alexandra , Knoke Thomas , Olschewski Roland
Published: February 2019

This paper aims at analyzing whether existing economic value estimates for forest ecosystem services (ES) might be transferred and used for valuation purposes elsewhere, and whether these data are appropriate for application in forest management. Many forest ES are public goods or positive externalities, and as a consequence they do not have a market price. The valuation of forest ES can provide important information for decision making in forest management and planning as well as in political processes, especially by allowing the comparison of different alternatives and helping set priorities for practical actions, as well as developing financial incentives or support mechanisms. We analyze whether an integrated economic valuation model for forest ES can be developed based on existing published data. To achieve this, we assess to which extent a benefit transfer could be expedient, and which challenges must be addressed. Based on a literature search, we compiled an extensive database of forest ES values. Given that these values vary substantially for the same ES, such a database alone does not seem useful to serve as a decision and management support tool. In addition, the available information mainly focuses on forests as such, and does not include desirable forest composition and management targets. If existing estimates should be transferred and used for forest management decisions, both the background conditions of the primary studies and the indicators used for valuation need to be specified in detail. The most expedient approach in this context seemed to be a valuation function transfer based on a broad set of indicators, offering the possibility to adapt the valuation function to changing background conditions.
Varietal Authentication of Extra Virgin Olive Oils by Triacylglycerols and Volatiles Analysis
Authors: Blasi Francesca , Pollini Luna , Cossignani Lina
Published: February 2019

In recent years, there is an increasing interest in high-quality extra virgin olive oils (EVOOs) produced from local cultivars. They have particular chemical/organoleptic characteristics and are frequently subjected to fraud, whereby the control of quality requires a powerful varietal check. In the present research, triacylglycerols (TAGs) and volatiles have been studied as chemical markers for the authentication of EVOO samples from four Italian varieties of Olea europea (Dolce Agogia, Frantoio, Leccino, and Moraiolo). The monocultivar EVOO samples have been subjected to a chemical–enzymatic chromatographic method in order to perform a stereospecific analysis, an important procedure for the characterization of TAG of food products. The results, combined with chemometric analysis (linear discriminant analysis, LDA), were elaborated in order to classify Italian EVOO monocultivar samples. In accordance with the total and intrapositional fatty acid (FA) composition of TAG fraction, the results were allowed to carry out a varietal discrimination. In addition, volatile compounds were also determined by solid-phase micro-extraction gas chromatography-mass spectrometry analysis. All EVOO samples were correctly classified when TAG stereospecific data and volatile results were elaborated by the LDA procedure, even if volatile compounds showed a higher discriminant power.
Platinum and Rhodium in Potato Samples by Using Voltammetric Techniques
Authors: Orecchio Santino , Amorello Diana
Published: February 2019

Potato is a starchy, tuberous crop from the perennial Solanum tuberosum having high nutritional values. This paper is the first analytical approach to quantify Pt and Rh in vegetal food. In this study a total of 38 different potato samples produced in Europe and one in Australia were investigated. Determinations of Pt and Rh in potato samples were carried out by Differential pulse voltammetry (DPV/a) for platinum and by Adsorptive stripping voltammetry (AdSV) for Rh using standard addition procedure. Because no certified reference potatoes containing platinum and rhodium are available, we used addition standard method. The quantification limits for Pt and Rh are 0.007 and 0.0008 μg kg−1 respectively. Considering all the potato samples, concentrations of Pt and Rh vary in the ranges from 0.007 to 109 μg kg−1 (sample no, 6 potatoes grown in Sicily) and from 0.0008 to 0.030 μg kg−1 (sample no. 3 of potatoes grown in Emilia Romagna), respectively. For both metals, in many cases the concentrations fall near the quantification limit. In all the samples, platinum is always more abundant than rhodium and their mean ratio is 14,500, which is much greater than that of the Earth’s crust (about 100).
Effect of the Roasting Conditions on the Physicochemical, Quality and Sensory Attributes of Coffee-Like Powder and Brew from Defatted Palm Date Seeds
Authors: Fikry Mohammad , Yusof Yus Aniza , Al-Awaadh Alhussein M. , Rahman Russly Abdul , Chin Nyuk Ling , Mousa Esraa , Chang Lee Sin
Published: February 2019

Developing a bioactive brew is a novel track for revalorization of palm date byproducts. The effect of roasting temperature (160, 180 and 200 °C ) and roasting time (10, 20 and 30 min) on the hardness of the roasted date seeds, moisture content of the defatted roasted date seed powder (DRDSP), bulk density of the DRDSP, color parameters of DRDSP, quality attributes (extraction yield, pH and browning index), the chemical properties (antioxidants and total phenolic content) and the sensory attributes (color, aroma, taste and overall preference) of the brew prepared from DRDSP was studied. The physicochemical, quality, and sensory attributes were found to be significantly influenced by the roasting temperature and time. Additionally, the models proposed could satisfactorily describe the changes in the different properties during the roasting process. The optimum conditions of the roasting process obtained using the superimposed contour plot were 199.9 °C and 21.5 min. In the longer term, the results of this study would be beneficial for the manufacturers of the date seeds powder and brew.
Comparison of the Transcriptomes and Proteomes of Serum Exosomes from Marek’s Disease Virus-Vaccinated and Protected and Lymphoma-Bearing Chickens
Authors: Neerukonda Sabari Nath , Tavlarides-Hontz Phaedra , McCarthy Fiona , Pendarvis Kenneth , Parcells Mark S.
Published: February 2019

Marek’s disease virus (MDV) is the causative agent of Marek’s disease (MD), a complex pathology of chickens characterized by paralysis, immunosuppression, and T-cell lymphomagenesis. MD is controlled in poultry production via vaccines administered in ovo or at hatch, and these confer protection against lymphoma formation, but not superinfection by MDV field strains. Despite vaccine-induced humoral and cell-mediated immune responses, mechanisms eliciting systemic protection remain unclear. Here we report the contents of serum exosomes to assess their possible roles as indicators of systemic immunity, and alternatively, tumor formation. We examined the RNA and protein content of serum exosomes from CVI988 (Rispens)-vaccinated and protected chickens (VEX), and unvaccinated tumor-bearing chickens (TEX), via deep-sequencing and mass spectrometry, respectively. Bioinformatic analyses of microRNAs (miRNAs) and predicted miRNA targets indicated a greater abundance of tumor suppressor miRNAs in VEX compared to TEX. Conversely, oncomiRs originating from cellular (miRs 106a-363) and MDV miRNA clusters were more abundant in TEX compared to VEX. Most notably, mRNAs mapping to the entire MDV genome were identified in VEX, while mRNAs mapping to the repeats flanking the unique long (IRL/TRL) were identified in TEX. These data suggest that long-term systemic vaccine-induced immune responses may be mediated at the level of VEX which transfer viral mRNAs to antigen presenting cells systemically. Proteomic analyses of these exosomes suggested potential biomarkers for VEX and TEX. These data provide important putative insight into MDV-mediated immune suppression and vaccine responses, as well as potential serum biomarkers for MD protection and susceptibility.
At the Beginning of the End and in the Middle of the Beginning: Structure and Maintenance of Telomeric DNA Repeats and Interstitial Telomeric Sequences
Authors: Aksenova Anna Y. , Mirkin Sergei M.
Published: February 2019

Tandem DNA repeats derived from the ancestral (TTAGGG)n run were first detected at chromosome ends of the majority of living organisms, hence the name telomeric DNA repeats. Subsequently, it has become clear that telomeric motifs are also present within chromosomes, and they were suitably called interstitial telomeric sequences (ITSs). It is well known that telomeric DNA repeats play a key role in chromosome stability, preventing end-to-end fusions and precluding the recurrent DNA loss during replication. Recent data suggest that ITSs are also important genomic elements as they confer its karyotype plasticity. In fact, ITSs appeared to be among the most unstable microsatellite sequences as they are highly length polymorphic and can trigger chromosomal fragility and gross chromosomal rearrangements. Importantly, mechanisms responsible for their instability appear to be similar to the mechanisms that maintain the length of genuine telomeres. This review compares the mechanisms of maintenance and dynamic properties of telomeric repeats and ITSs and discusses the implications of these dynamics on genome stability.
Screening for High Risk of Sleep Apnea in an Ambulatory Care Setting in Saudi Arabia
Authors: Ahmad Adeel Nazir , McLeod Geraldine , Al Zahrani Nada , Al Zahrani Haneen
Published: February 2019

Sleep apnea is a potentially serious but under-diagnosed sleep disorder. Saudi Arabia has a high prevalence of hypertension, diabetes, obesity, and smoking, which are all major risk factors for sleep apnea. However, few studies report screening for sleep apnea in Saudi Arabia. A three-month prospective, questionnaire-based study, using the Berlin Questionnaire (BQ) and the Epworth Sleepiness Scale (ESS), screened 319 patients attending a family medicine clinic in Saudi Arabia for risk of sleep apnea. The results showed that when using the BQ and the ESS, 95 (29.8%) and 102 (32.0%) respondents were at high risk of sleep apnea. Taken together, the BQ and the ESS combined measure showed that 41 (12.9%) respondents were classified as high risk for sleep apnea. Logistic regression revealed that the high risk of sleep apnea was statistically significantly (p < 0.05) associated with respondent characteristics of obesity and hypertension. No associations were found between high risk for sleep apnea and: Smoking, diabetes mellitus, hypothyroidism or hyperlipidemia. Screening for sleep apnea using the BQ and ESS questionnaires, particularly among those who are obese or hypertensive, can be a fast, valid and acceptable way of alerting the physician to this disorder among patients.
Decisions of the Dual-Channel Supply Chain under Double Policy Considering Remanufacturing
Authors: Feng Dingzhong , Ma Lei , Ding Yangke , Wu Guanghua , Zhang Ye
Published: February 2019

Considering the preference of green consumers for remanufactured products, a dual-sale-channel supply chain model with government non-intervention, government remanufacturing subsidy policy, and carbon tax policy is constructed, respectively. The difference of the optimal decision between the firm and the government under the two policies is discussed in this paper. Meanwhile, we analyze the influence of green consumers on the government’s optimal decision, based on social welfare maximization. It is found that without government intervention, social welfare is the lowest. The carbon tax policy is better when the proportion of green consumers and the environmental coefficient are extreme or moderate at the same time. Otherwise, the subsidy policy is better. The carbon tax policy is more effective than the subsidy policy in controlling carbon emissions. Profit-sharing contracts should be established by enterprises and governments to achieve win–win results.
Efficacy of Nutrition and WASH/Malaria Educational Community-Based Interventions in Reducing Anemia in Preschool Children from Bengo, Angola: Study Protocol of a Randomized Controlled Trial
Authors: Fançony Cláudia , Soares Ânia , Lavinha João , Barros Henrique , Brito Miguel
Published: February 2019

Angola reports one of the highest infant mortality rates in the world, and anemia represents one of its important causes. Recent studies, in under-five children from the Bengo province of Angola, described high prevalence’s, suggesting malaria, undernutrition and urogenital schistosomiasis as important contributors for the occurrence and spatial variations of anemia. Educational community-based interventions, either in Nutrition and Water, Sanitation, Hygiene and Malaria are recommended to correct anemia. Herein, we designed a cluster-randomized controlled trial to study the efficacy of two educational-plus-therapeutic interventions in the reduction of anemia: one in nutrition and the other in WASH/Malaria. Socioeconomic, nutritional, anthropometric, parasitological and biochemical data will be collected from all willing-to-participate children, aging under four and resident in the Health Research Center of Angola study area. Considering the multifactorial causes of this condition, determining the efficacy of both interventions might help documenting weaknesses and opportunities for planning integrated strategies to reduce anemia.
A Projection of Future Hospitalisation Needs in a Rapidly Ageing Society: A Hong Kong Experience
Authors: Wu Xueyuan , Law Chi-kin , Yip Paul Siu Fai
Published: February 2019

To assess the impact of ageing on hospitalisation in a rapidly ageing society. A study using retrospective and prospective data was conducted using hospitalisation data with age-specific admission rates in the period from 2001–2010 and demographic data from the period of 2001–2066 by the United Nations. The Hong Kong Special Administrative Region (SAR) with a 7 million population experiences extreme low fertility (1.1 children per woman) and long life expectancy (84 years old). Days of hospitalisation: For the period 2010–2066, the length of stay (LOS) in the age group 85+ is projected to increase by 555.3% while the LOS for the whole population is expected to increase by only 134.4% and by ageing only. In 2010, the proportion in the LOS contributed to by the oldest age group (85+) was 15%. In 2066, this proportion is projected to nearly triple (42%). Around 70% of the projected days of hospitalisation would be taken by people aged 75 years and above. It is projected that this phenomenon would be converted to a more balanced structure when the demographic transition changes into a more stable distribution. Apparently, the impact of ageing on the public hospital system has not been well understood and prepared. The determined result provides insight into monitoring the capacity of the hospital system to cope with a rapidly changing demographic society. It provides empirical evidence of the impact of ageing on the public hospitalisation system. It gives a long term projection up to the year 2066 while the situation would be different from the transient period of 2016–2030. The analysis adopts a fixed rate approach, which assumes the LOS to be only driven by demographic factors, while any improvements in health technologies and health awareness are not accounted for. Only inpatient data from the Hospital Authority were used, nonetheless, they are the best available for the study. Due to the limitation of data, proximity to death is not controlled in conducting this analysis.
Prototyping of Environmental Kit for Georeferenced Transient Outdoor Comfort Assessment
Authors: Nouman Ahmad Saleem , Chokhachian Ata , Santucci Daniele , Auer Thomas
Published: February 2019

Environmental data acquisition tools are broadly used for climate monitoring and urban comfort assessment followed by data mining and sensing techniques for putting into evidence the relationship between environmental qualities of urban spaces and human well-being. Within this context, an environmental toolkit is a fundamental tool to evaluate transient outdoor comfort. This study explains the prototyping and validation of a mobile environmental sensor kit. The results show the prototype has reasonable accuracy despite its affordability with respect to industrial sensors.
Diabetes Impairs Angiogenesis and Induces Endothelial Cell Senescence by Up-Regulating Thrombospondin-CD47-Dependent Signaling
Authors: Bitar Milad S.
Published: February 2019

Endothelial dysfunction, impaired angiogenesis and cellular senescence in type 2 diabetes constitute dominant risk factors for chronic non-healing wounds and other cardiovascular disorders. Studying these phenomena in the context of diabetes and the TSP1-CD-47 signaling dictated the use of the in vitro wound endothelial cultured system and an in vivo PVA sponge model of angiogenesis. Herein we report that diabetes impaired the in vivo sponge angiogenic capacity by decreasing cell proliferation, fibrovascular invasion and capillary density. In contrast, a heightened state of oxidative stress and elevated expression of TSP1 and CD47 both at the mRNA and protein levels were evident in this diabetic sponge model of wound healing. An in vitro culturing system involving wound endothelial cells confirmed the increase in ROS generation and the up-regulation of TSP1-CD47 signaling as a function of diabetes. We also provided evidence that diabetic wound endothelial cells (W-ECs) exhibited a characteristic feature that is consistent with cellular senescence. Indeed, enhanced SA-β-gal activity, cell cycle arrest, increased cell cycle inhibitors (CKIs) p53, p21 and p16 and decreased cell cycle promoters including Cyclin D1 and CDK4/6 were all demonstrated in these cells. The functional consequence of this cascade of events was illustrated by a marked reduction in diabetic endothelial cell proliferation, migration and tube formation. A genetic-based strategy in diabetic W-ECs using CD47 siRNA significantly ameliorated in these cells the excessiveness in oxidative stress, attenuation in angiogenic potential and more importantly the inhibition in cell cycle progression and its companion cellular senescence. To this end, the current data provide evidence linking the overexpression of TSP1-CD47 signaling in diabetes to a number of parameters associated with endothelial dysfunction including impaired angiogenesis, cellular senescence and a heightened state of oxidative stress. Moreover, it may also point to TSP1-CD47 as a potential therapeutic target in the treatment of the aforementioned pathologies.
Role of Immunogenetics in the Outcome of HCMV Infection: Implications for Ageing
Authors: Aiello Anna , Accardi Giulia , Candore Giuseppina , Caruso Calogero , Colomba Claudia , Di Bona Danilo , Duro Giovanni , Gambino Caterina Maria , Ligotti Mattia Emanuela , Pandey Janardan P.
Published: February 2019

The outcome of host-virus interactions is determined by a number of factors, some related to the virus, others to the host, such as environmental factors and genetic factors. Therefore, different individuals vary in their relative susceptibility to infections. Human cytomegalovirus (HCMV) is an important pathogen from a clinical point of view, as it causes significant morbidity and mortality in immunosuppressed or immunosenescent individuals, such as the transplanted patients and the elderly, respectively. It is, therefore, important to understand the mechanisms of virus infection control. In this review, we discuss recent advances in the immunobiology of HCMV-host interactions, with particular emphasis on the immunogenetic aspects (human leukocyte antigens, HLA; killer cell immunoglobulin-like receptors, KIRs; immunoglobulin genetic markers, GM allotypes) to elucidate the mechanisms underlying the complex host-virus interaction that determine various outcomes of HCMV infection. The results, which show the role of humoral and cellular immunity in the control of infection by HCMV, would be valuable in directing efforts to reduce HCMV spurred health complications in the transplanted patients and in the elderly, including immunosenescence. In addition, concerning GM allotypes, it is intriguing that, in a Southern Italian population, alleles associated with the risk of developing HCMV symptomatic infection are negatively associated with longevity.
Evaluation of Storage Tubes for Combined Analysis of Circulating Nucleic Acids in Liquid Biopsies
Authors: Ward Gahlawat Aoife , Lenhardt Judith , Witte Tania , Keitel Denise , Kaufhold Anna , Maass Kendra K , Pajtler Kristian W , Sohn Christof , Schott Sarah
Published: February 2019

In the last decade, circulating nucleic acids such as microRNAs (miRNAs) and cell-free DNA (cfDNA) have become increasingly important in serving as potential novel biomarkers for a variety of human diseases. If cell-free nucleic acids are to become routinely used in diagnostics, the difference in plasma miRNA and cfDNA levels between healthy and diseased subjects must exceed pre-analytical and analytical variability. Until now, few studies have addressed the time limitations of pre-processing or explored the potential use of long-term blood storage tubes, which might need to be implemented in real-life diagnostics. In this study, we analyzed the stability of four breast cancer-associated miRNAs and two cancer-associated genes under various storage conditions, to test their limitations for potential application in clinical diagnostics. In two consecutive experiments, we tested the limits of conventional EDTA tubes, as well as long-term storage blood collection tubes (BCTs) from four different manufacturers. We found that circulating miRNAs are relatively stable when stored in EDTA monovettes for up to 12 h before processing. When stored in BCTs, circulating miRNAs and cfDNA are stable for up to 7 days, depending on the manufacturer. Norgen tubes were superior for cfDNA yield, while Streck tubes performed the worst in our study with hemolysis induction. In conclusion, plasma prepared from whole blood is suitable for the quantification of both cf-miRNAs and cfDNA simultaneously.
Long-Term Efficacy and Safety of Pemafibrate, a Novel Selective Peroxisome Proliferator-Activated Receptor-α Modulator (SPPARMα), in Dyslipidemic Patients with Renal Impairment
Authors: Yokote Koutaro , Yamashita Shizuya , Arai Hidenori , Araki Eiichi , Suganami Hideki , Ishibashi Shun
Published: February 2019

Pemafibrate (K-877) is a novel selective peroxisome proliferator-activated receptor-α modulator (SPPARMα) with a favorable benefit-risk balance. Previous clinical trials of pemafibrate used stringent exclusion criteria related to renal functions. Therefore, we investigated its safety and efficacy in a broader range of patients, including those with chronic kidney disease (CKD). In this multicenter, single-arm, open-label, phase III trial, 0.2–0.4 mg/day pemafibrate was administered for 52 weeks to 189 patients with hypertriglyceridemia and an estimated glomerular filtration rate (eGFR) ≥ 45 mL/min/1.73 m2 on statin or regardless of eGFR when statin was not administered. Post-hoc analyses were performed on subgroups stratified by baseline eGFR. Triglyceride levels decreased by 45.9% at week 52 (last-observation-carried-forward). These reductions were not correlated with baseline eGFR. The eGFR < 30 mL/min/1.73 m2 subgroup showed the greatest reduction in chylomicron, very low-density lipoprotein, small low-density lipoprotein cholesterol levels, and an increase in high-density lipoprotein cholesterol levels. The incidences of adverse events and adverse drug reactions were 82.0% and 31.7%, respectively, and these were not associated with baseline eGFR. In CKD patients, pemafibrate blood concentrations were not elevated. Pemafibrate showed a good safety profile and efficacy in correcting lipid abnormalities in a broad range of patients, including those with CKD.
Artificial Intelligence Hits the Barrier of Meaning
Authors: Mitchell Melanie
Published: February 2019

Today’s AI systems sorely lack the essence of human intelligence: Understanding the situations we experience, being able to grasp their meaning. The lack of humanlike understanding in machines is underscored by recent studies demonstrating lack of robustness of state-of-the-art deep-learning systems. Deeper networks and larger datasets alone are not likely to unlock AI’s “barrier of meaning”; instead the field will need to embrace its original roots as an interdisciplinary science of intelligence.
Efficacy of Combinations of Methoprene and Deltamethrin as Long-Term Commodity Protectants
Authors: Arthur Frank H.
Published: February 2019

Wheat, corn, and brown rice were treated with different combinations of a deltamethrin suspension concentrate (SC) formulation and a new emulsifiable concentrate (EC) formulation, at rates of either 0.5 or 1.0 ppm alone or in combination with 1.25 and 2.5 ppm methoprene (10 treatments in all, including an untreated control). Treated commodities were stored at ambient conditions on the floor of an empty grain bin in Manhattan, KS, USA, in 5-kg lots for individual replicates. The commodities were sampled and bio-assayed every three months for 15 months by exposing 10 mixed-sex parental adults of selected adult stored product insects on 70–80 g of the commodity. For all treatments, there was no regression of declining efficacy with respect to the month. Therefore, the data were combined for analysis. On wheat and brown rice, there was no reproduction of Rhyzopertha dominica (Fabricius) in any of the treatments, and there was no weight loss in either commodity that was caused by feeding of the parental adults or developing progeny. There was reproduction of Sitophilus oryzae (L.) on wheat but, for several combinations, the EC formulation gave better suppression of progeny compared to the standard SC. However, on brown rice, only the combination of 1.0 ppm deltamethrin EC and 2.5 ppm methoprene was different than other treatments with respect to progeny development, sample weight loss caused by feeding, and weight of the feeding damage itself. Progeny production was correlated with grain damage. No progeny of Tribolium castaneum (Herbst) developed on the treated corn, but there was some variation in insect damage, with less damage in those treatments involving the EC formulation. Progeny production of Sitophilus zeamais (Motschulsky) was at the lowest in the combination of 1.0 ppm deltamethrin EC and 2.5 ppm methoprene. The resulting insect damage was the lowest in this combination as well. Results of this study were used by the registrant (Central Life Sciences) in the United States (US) to modify the commercial formulation to replace the deltamethrin SC with the EC, at label rates of either 0.5 ppm EC + 1.25 ppm methoprene, or 1.0 ppm EC + 2.5 ppm methoprene, on wheat, corn, and rice.
Long-Term Outcome of Potential Celiac Disease in Genetically at-Risk Children: The Prospective CELIPREV Cohort Study
Authors: Lionetti Elena , Castellaneta Stefania , Francavilla Ruggiero , Pulvirenti Alfredo , Naspi Catassi Giulia , Catassi Carlo ,   The SIGENP Working Group of Weaning and CD Risk
Published: February 2019

Background: The long-term outcome of potential celiac disease (CD) is still a debated issue. We aimed to evaluate the progression of potential CD versus overt CD after 10-years of follow-up in a cohort of children genetically predisposed to CD. Methods: The CELIPREV study is prospectively following from birth 553 children with CD-predisposing HLA genes. Children with a diagnosis of potential CD continued to receive a normal diet and repeated the serological screening for CD every year. An intestinal biopsy was taken in presence of persistent positive serology. Results: Overall, 26 (4.7%) children received a diagnosis of potential CD (50% females, median age 24 months). All children were symptom-free. Twenty-three children continued a gluten-containing diet; at 10 years from the first biopsy, three children developed overt CD (13%), 19 (83%) became antibodies negative at 1 year from the first biopsy and remained negative up to 10 years of follow-up and one subject (4%) had fluctuating antibody course with transiently negative values and persistently negative biopsy. Conclusions: In children genetically predisposed to CD with a diagnosis of potential CD the risk of progression to overt CD while on a gluten-containing diet is very low in the long-term.
CHADS2, CHA2DS2ASc, and New ABCD Scores Predict the Risk of Peripheral Arterial Disease in Patients with Sleep Apnea
Authors: Wu Kuan-Li , Kuo Chia-Yu , Tsai Yu-Chen , Hung Jen-Yu , Sheu Chau-Chyun , Yang Chih-Jen , Hsu Chung-Yao , Wu Meng-Ni , Tsai Ming-Ju
Published: February 2019

The association between sleep apnea (SA) and peripheral artery disease (PAD) remains debatable, and there is no clinical tool to predict incident PAD in SA patients. The CHADS2 score has been found useful in predicting PAD risk. This study was designed to investigate the association between these diseases and the usefulness of CHADS2 and CHA2DS2ASc scores in predicting subsequent PAD in SA patients. From a population-based database of one-million representative subjects, adult patients with SA diagnosis were enrolled as the suspected SA group, and those having SA diagnosis after polysomnography were further extracted as the probable SA group. Twenty sex- and age-matched control subjects were randomly selected for each SA patients. The occurrence of PAD after SA was taken as the primary endpoint. Totally, 10,702 and 4242 patients were enrolled in the suspected and probable SA groups, respectively. The cumulative incidence of PAD was similar between SA patients and the corresponding control groups. Multivariable Cox regression analyses showed that SA was not an independent risk factor for subsequent PAD. Sensitivity analyses using propensity score-matched cohorts showed consistent results. Furthermore, in stratifying the SA patients by CHADS2, CHA2DS2ASc, or a newly-proposed ABCD (composed of Age, high Blood pressure, Cerebral vascular disease, and Diabetes mellitus) score, patients with higher scores predicted higher risks of subsequent PAD, while the ABCD score appeared to be the most robust. Aggressive risk modification is suggested to reduce the subsequent PAD risk in SA patients with a higher CHADS2, CHA2DS2ASc, or ABCD score.
Interventions to Promote Patient Utilization of Cardiac Rehabilitation: Cochrane Systematic Review and Meta-Analysis
Authors: Santiago de Araújo Pio Carolina , Chaves Gabriela , Davies Philippa , Taylor Rod , Grace Sherry
Published: February 2019

Too few patients utilize cardiac rehabilitation (CR), despite its benefits. The Cochrane review assessing the effectiveness of interventions to increase CR utilization (enrolment, adherence, and completion) was updated. A search was performed through July 2018 of the Cochrane and MEDLINE (Medical Literature Analysis and Retrieval System Online) databases, among other sources. Randomized controlled trials in adults with myocardial infarction, angina, revascularization, or heart failure were included. Interventions had to aim to increase utilization of comprehensive phase II CR. Two authors independently performed all stages of citation processing. Following the random-effects meta-analysis, meta-regression was undertaken to explore the impact of pre-specified factors. Twenty-six trials with 5299 participants were included (35.8% women). Low-quality evidence showed an effect of interventions in increasing enrolment (risk ratio (RR) = 1.27, 95% confidence interval (CI) = 1.13–1.42). Meta-regression analyses suggested that the intervention deliverer (nurse or allied healthcare provider, p = 0.02) and delivery format (face-to-face, p = 0.01) were influential in increasing enrolment. There was low-quality evidence that interventions to increase adherence were effective (standardized mean difference (SMD) = 0.38, 95% CI = 0.20–0.55), particularly where remotely-offered (SMD = 0.56, 95% CI = 0.36–0.76). There was moderate-quality evidence that interventions to increase program completion were effective (RR = 1.13, 95% CI = 1.02–1.25). There are effective interventions to increase CR utilization, but more research is needed to establish specific, implementable materials and protocols, particularly for completion.
Application of Diagnostic Interview for Internet Addiction (DIA) in Clinical Practice for Korean Adolescents
Authors: Ryu Hyera , Lee Ji Yoon , Choi A Ruem , Chung Sun Ju , Park Minkyung , Bhang Soo-Young , Kwon Jun-Gun , Kweon Yong-Sil , Choi Jung-Seok
Published: February 2019

The increased prevalence of Internet Gaming Disorder (IGD) and the inclusion of IGD in DSM-5 and ICD-11 emphasizes the importance of measuring and describing the IGD symptoms. We examined the psychometric properties of the Diagnostic Interview for Internet Addiction (DIA), a semi-structured diagnostic interview tool for IGD, and verified the application of DIA in clinical practice for Korean adolescents. The DIA is conducted in a manner that interviews both adolescents and their caregivers, and each item has a standardized representative question and various examples. It consists of 10 items based on the DSM-5 IGD diagnostic criteria, which is cognitive salience, withdrawal, tolerance, difficulty in regulating use, loss of interest in other activities, persistent use despite negative results, deception regarding Internet/games/SNS use, use of Internet/games/SNS to avoid negative feelings, interference with role performance, and craving. The study included 103 adolescents divided into three subgroups (mild risk, moderate risk, and addicted group) based on the total score of DIA. Demographic and clinical characteristics were compared among the DIA subgroups using the chi-square test and analysis of variance (ANOVA), and correlation analysis was used to examine the associations of IGD symptoms with clinical variables (e.g., impulsivity, aggression, depression, anxiety, self-esteem). The DIA total score was significantly correlated with Internet and smartphone addiction, depression, state anxiety, self-esteem, impulsivity, aggression, and stress. Furthermore, the moderate risk and addicted group showed significantly higher levels of Internet and smartphone addiction, anxiety, depression, impulsivity, aggression, stress, and lower self-esteem compared with the mild risk group. The Junior Temperament and Character Inventory (JTCI), which measures temperament and character traits, revealed that the mild risk group had higher levels of persistence and self-directedness than did the addicted group. Our findings confirmed the psychometric properties of DIA and the application of the DIA classifications in Korean adolescents.
DoS Attack Detection and Path Collision Localization in NoC-Based MPSoC Architectures
Authors: Chaves Cesar Giovanni , Azad Siavoosh Payandeh , Hollstein Thomas , Sepúlveda Johanna
Published: February 2019

Denial of Service (DoS) attacks are an increasing threat for Multiprocessor System-on-Chip (MPSoC) architectures. By exploiting the shared resources on the chip, an attacker is able to prevent completion or degrade the performance of a task. This is extremely dangerous for MPSoCs used in critical applications. The Network-on-Chip (NoC), as a central MPSoC infrastructure, is exposed to this attack. In order to maintain communication availability, NoCs should be enhanced with an effective and precise attack detection mechanism that allows the triggering of effective attack mitigation mechanisms. Previous research works demonstrate DoS attacks on NoCs and propose detection methods being implemented in NoC routers. These countermeasures typically led to a significantly increased router complexity and to a high degradation of the MPSoC’s performance. To this end, we present two contributions. First, we provide an analysis of information that helps to narrow down the location of the attacker in the MPSoC, achieving up to a 69% search space reduction for locating the attacker. Second, we propose a low cost mechanism for detecting the location and direction of the interference, by enhancing the communication packet structure and placing communication degradation monitors in the NoC routers. Our experiments show that our NoC router architecture detects single-source DoS attacks and determines, with high precision, the location and direction of the collision, while incurring a low area and power overhead.
Shape-Memory Assisted Scratch-Healing of Transparent Thiol-Ene Coatings
Authors: Lazauskas Algirdas , Jucius Dalius , Baltrušaitis Valentinas , Gudaitis Rimantas , Prosyčevas Igoris , Abakevičienė Brigita , Guobienė Asta , Andrulevičius Mindaugas , Grigaliūnas Viktoras
Published: February 2019

A photopolymerizable thiol-ene composition was prepared as a mixture of pentaerythritol tetrakis(3-mercaptopropionate) (PETMP) and 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione (TTT), with 1 wt. % of 2,2-dimethoxy-2-phenylacetophenone (DMPA) photoinitiator. A systematic analytical analysis that investigated the crosslinked PETMP-TTT polymer coatings employed Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, differential scanning calorimetry, thermogravimetric analysis, pencil hardness, thermo-mechanical cyclic tensile, scratch testing, and atomic force microscopy. These coatings exhibited high optical transparency and shape-memory that assisted scratch-healing properties. Scratches produced on the PETMP-TTT polymer coatings with different constant loadings (1.2 N, 1.5 N, and 2.7 N) were completely healed after the external stimulus was applied. The strain recovery ratio and total strain recovery ratio for PETMP-TTT polymer were found to be better than 94 ± 1% and 97 ± 1%, respectively. The crosslinked PETMP-TTT polymer network was also capable of initiating scratch recovery at ambient temperature conditions.
Super-Capacitive Performance of Manganese Dioxide/Graphene Nano-Walls Electrodes Deposited on Stainless Steel Current Collectors
Authors: Amade Roger , Muyshegyan-Avetisyan Arevik , Martí González Joan , Pérez del Pino Angel , György Eniko , Pascual Esther , Andújar José Luís , Bertran Serra Enric
Published: February 2019

Graphene nano-walls (GNWs) are promising materials that can be used as an electrode in electrochemical devices. We have grown GNWs by inductively-coupled plasma-enhanced chemical vapor deposition on stainless steel (AISI304) substrate. In order to enhance the super-capacitive properties of the electrodes, we have deposited a thin layer of MnO2 by electrodeposition method. We studied the effect of annealing temperature on the electrochemical properties of the samples between 70 °C and 600 °C. Best performance for supercapacitor applications was obtained after annealing at 70 °C with a specific capacitance of 104 F·g−1 at 150 mV·s−1 and a cycling stability of more than 14k cycles with excellent coulombic efficiency and 73% capacitance retention. Electrochemical impedance spectroscopy, cyclic voltammetry, and galvanostatic charge/discharge measurements reveal fast proton diffusion (1.3 × 10−13 cm2·s−1) and surface redox reaction after annealing at 70 °C.
Reactive Compatibilization of PLA/PA11 Blends and Their Application in Additive Manufacturing
Authors: Rasselet Damien , Caro-Bretelle Anne-Sophie , Taguet Aurélie , Lopez-Cuesta José-Marie
Published: February 2019

The aim of this work was to study the properties of polylatic acid/polyamide 11 (PLA/PA11) blends compatibilized with a multifunctionalized epoxide, Joncryl®, and to evaluate the performance of such blends processed by Fused Deposition Modeling (FDM) 3D printing, compared to those produced by injection molding method. Blends containing different Joncryl contents from 0.5 to 3 wt% were prepared by twin-screw extrusion. Evaluation of thermal, rheological and mechanical properties of such blends proved that Joncryl acted as a compatibilizer. Results showed that Joncryl effects on blends properties were improved with increasing its content. A significant reduction of PA11 dispersed phases diameter and an improvement of tensile properties with a ductile behavior were achieved for the highest Joncryl contents. A significant elongation of PA11 dispersed phases was observed into FDM filaments and dog bone shaped specimens produced thereafter. Despite this peculiar morphology, FDM printed samples exhibited only enhanced stiffness but poor reinforcement and elongation at break in comparison with injected ones.
Manufacturing Dense Thick Films of Lunar Regolith Simulant EAC-1 at Room Temperature
Authors: Nieke Philipp , Kita Jaroslaw , Häming Marc , Moos Ralf
Published: February 2019

The Aerosol Deposition (AD, also known as gas kinetic spraying or vacuum deposition) method is a rather novel coating process to produce dense thick films directly from dry ceramic (or metal) powders on a variety of substrates without any heat treatment. Because of the similarity of the up to now used powders and lunar regolith, it is imaginable to use AD systems for future in situ resource utilization missions on the Moon planned by several space agencies. To test the feasibility of such an endeavor, the processability of lunar mare simulant EAC-1 by the AD method has been examined in this study. Three regolith films with an area of 25 × 10 mm2, and thicknesses between 2.50 µm and 5.36 µm have been deposited on steel substrates using a standard AD setup. Deposited films have been investigated by Laser Scanning Microscopy (LSM) and Scanning Electron Microscopy (SEM). Moreover, the roughness and Vickers hardness of the deposited films and the underlying substrates have been measured. It has been shown that dense consolidated films of regolith simulant can be produced within minutes by AD. The deposited films show a higher roughness and, on average, a higher hardness than the steel substrates. Since on the Moon, naturally available regolith powders are abundant and very dry, and since the required process vacuum is available, AD appears to be a very promising method for producing dense coatings in future Moon exploration and utilization missions.
The Significance of Entropy in Grain Boundary Segregation
Authors: Lejček Pavel , Hofmann Siegfried , Paidar Václav
Published: February 2019

The role of entropy in materials science is demonstrated in this report in order to establish its importance for the example of solute segregation at the grain boundaries of bcc iron. We show that substantial differences in grain boundary chemistry arise if their composition is calculated with or without consideration of the entropic term. Another example which clearly documents the necessity of implementing the entropic term in materials science is the enthalpy-entropy compensation effect. Entropy also plays a decisive role in the anisotropy of grain boundary segregation and in interface characterization. The consequences of the ambiguous determination of grain boundary segregation on the prediction of materials behavior are also briefly discussed. All the mentioned examples prove the importance of entropy in the quantification of grain boundary segregation and consequently of other materials properties.