bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcultural skills for early childhood professionals
Authors: Radjack Rahmeth , Bossuroy Muriel , Camara Hawa , Touhami Fatima , Ogrizek Anaïs , Rodriguez Juliette , Robin Marion , Moro Marie Rose
Published: April 2023

Context

Transcultural skills are especially useful for those involved in the perinatal period, when parents and babies must adapt to one another in a setting of migration a long a focus of transcultural clinical practice.

Objective

The aim of this article is to provide useful transcultural skills for any health care worker (e.g., psychologists, child psychiatrists, midwives, family doctors, pediatricians, specialized child-care attendants, and social workers) who provide care or support to families during the perinatal period. It highlights the cultural aspects requiring attention in relation to representations of pregnancy, children’s needs, obstetric complications, and postnatal problems. Taking into account the impact of culture on clinical evaluation and treatment can enable professionals to distinguish what involves cultural representations of pregnancy, babies, and sometimes of disease from what is associated with interaction disorders or maternal psychopathology.

Methods

After explaining the relevance of transcultural clinical practices to provide migrant mothers with better support, we describe 9 themes useful to explore from a transcultural perspective. This choice is based on the transcultural clinical practice in our specialized department.

Results

The description of these 9 themes is intended to aid in their pragmatic application and is illustrated with short clinical vignettes for specific concepts. We describe situations that are extreme but often encountered in liaison transcultural clinical practice for maternity wards: perinatal mourning with cultural coding, mediation in refusal of care, cultural misunderstandings, situations of complex trauma and of multiple contextual vulnerabilities, and difficulties associated with acculturation.

Discussion

The transcultural levers described here make it possible to limit cultural misunderstandings and to promote the therapeutic alliance. It presupposes the professionals will concomitantly analyze their cultural countertransference and acquire both the knowledge and know-how needed to understand the elements of cultural, political, and social issues needed to develop clinical finesse.

Conclusion

This combined theoretical-clinical article is intended to be pedagogical. It provides guidelines for conducting transcultural child psychiatry/psychological interviews in the perinatal period aimed at both assessment and therapy.
The experiences of East Asian dementia caregivers in filial culture: a systematic review and meta-analysis
Authors: Wang Qian , Xiao Xiaohua , Zhang Jin , Jiang Dongdong , Wilson Amanda , Qian Beiran , Song Peige , Yang Qian
Published: April 2023

Background

With the aging population in East Asia, the prevalence of dementia and the need for patient care is increasing. Family caregivers of people with dementia are at risk of physical and mental health problems. Filial piety culture regulates relationships within East Asian families and effects the well-being and behavior of dementia family caregivers (CGs).

Objective

To systematically assess the experience of East Asian dementia caregivers in filial culture. Methods: Electronic databases, including MEDLINE, APA PsycINFO, CINAHL (via EBSCOhost), Web of Science, and Cochrane Library, were searched for relevant studies up to July 2021. Only original articles were included.

Results

Thirteen eligible studies were included, of which eight were qualitative and five were quantitative. Meta-analysis showed a negative association (r = −0.18, 95%CI [−0.28, −0.08]) between filial culture and caregiver burden. The quantitative studies identified four themes related to dementia caregivers’ experiences: (1) Recognition and understanding of filial piety as part of cultural identity, (2) Role transitions- from child to CG, (3) Filial piety’s constraints on CGs; (4) CGs’ self-compassion through changing cultural norms of filial practice.

Conclusion

Filial culture influences the whole process behind caregiving for East Asian dementia caregivers. At the same time, cultural transition has also brought about new connotations and practices to filial culture.
Migrants’ human rights and health protection during the COVID-19 pandemic in the Mediterranean Sea: what we have learnt from direct inspections in two Italian hotspots
Authors: Pomara Cristoforo , Zappalà Salvatore Angelo , Salerno Monica , Sessa Francesco , Esposito Massimiliano , Cocimano Giuseppe , Ippolito Salvatore , Miani Alessandro , Missoni Eduardo , Piscitelli Prisco
Published: April 2023

This study aims to assess the situation of Italian hotspots for migrant reception during the COVID-19 pandemic, and specifically analyzing the situation of two hotspots located in the Sicily Region (Pozzallo harbor and Lampedusa Island), to identify critical issues. At the same time, we hypothesize solutions to guarantee the respect of human rights and suggest an operational protocol to be applied in similar situations, considering that the migration phenomenon is increasing and involving new geographical areas. Based on data obtained through the site inspections, the facilities of Pozzallo and Lampedusa exceeded their capacity to adequately contain the spread of the SARS-CoV-2 infection. Considering these findings, we suggest a practical workflow summarizing the main actions that should be applied to contain COVID-19, or other infectious disease, spreading in hotspots for migrants. The impact of the COVID-19 pandemic on migrants has received limited attention, although the migration phenomenon did not slow down during the pandemic period. Regarding the risk of spreading infectious diseases such as COVID-19, it is necessary that those countries who are most exposed to migration flows, such as Italy, plan dedicated strategies to minimize the possibility of transmission of SARS-CoV-2, using adequate protocols to monitor the possible insurgence of variants of interest (VOIs) or variants of concern (VOCs). Finally, it is important to state that these suggestions could be applied in any future pandemics.
Sociodemographic and behavioral factors associated with controlled hypertension after 9 years of observation of a PURE Poland cohort study
Authors: Zatońska Katarzyna , Basiak-Rasała Alicja , Połtyn-Zaradna Katarzyna , Gaweł-Dąbrowska Dagmara , Wołyniec Maria , Karczewski Maciej , Szuba Andrzej
Published: April 2023

Introduction

Despite some improvement in awareness and treatment of hypertension, blood pressure control is still below expectations in Poland. The aim of the study was to analyze the secular trend of hypertension prevalence in the PURE Poland cohort study over 9 years of observation and to analyze factors associated with controlled HT.

Methods

The study group consisted of 1,598 participants enrolled in a Prospective Urban and Rural Epidemiological Study (PURE), who participated both in baseline (2007–2010) and 9-year follow-up (2016–2019). Hypertension was ascertained on the basis of (1) self-reported hypertension previously diagnosed by the physician, (2) self-reported anti-hypertensive medication, and/or (3) an average of two blood pressure measurements ≥140 mmHg systolic BP and/or ≥90 mmHg diastolic BP.

Results

The prevalence of hypertension increased from 69.4% at baseline to 85.9% at 9-year follow-up. The chance of HT was 8.6-fold higher in the oldest vs. the youngest age group [OR 8.55; CI 4.47–16.1]. Male sex increased the chance for hypertension over 3-fold [OR 3.23; CI 2.26–4.73]. Obesity, according to BMI, increased the chance of HT 8-fold [OR 8.01; CI 5.20–12.8] in comparison with normal body weight. Male sex decreased the chance of controlled HT after 9 years [OR 0.68; CI 0.50–0.92]. There was no statistically significant association between controlled HT and age or place of residence. Higher and secondary education increased the chance of controlled HT over 2-fold in comparison with primary education [OR 2.35; CI 1.27–4.34, OR 2.34; CI 1.33–4.11]. Obesity significantly decreased the chance of controlled HT after 9 years in comparison with normal body weight [OR 0.54; CI 0.35–0.83].

Conclusion

Factors significantly increasing the chance for controlled hypertension after 9 years were female sex, secondary and tertiary education, normal body weight, and avoiding alcohol drinking. Changes in lifestyle, with special emphasis on maintaining normal body weight, should be the basis of prevention and control of HT.
Technique and maximal skiing speed for youth cross-country skiing performance
Authors: Stöggl Roland , Müller Erich , Stöggl Thomas
Published: April 2023

Introduction

Numerous researches concentrate on examining and preparing high-level male cross-country skiers, with a significant number of tests being conducted on roller skis. However, there is a scarcity of research on the testing and preparation of younger male and female athletes ranging from 10 to 16 years old. The main purpose of this research was to determine if certain cross-country (XC) skiing tests and maturity status are indicators of performance in youth cross-country skiing; to examine any differences in performance between young males and females; and to establish non-invasive diagnostic tools for assessing performance.

Methods

Fifty-eight young XC skiers (36 boys; 12.88 ± 1.19 yrs and 22 girls; 12.79 ± 1.09 yrs) performed specific XC skiing maximal speed tests consisting of short (50 m) flat and uphill distances (30/40 m). Results were correlated with on snow XC skiing performance (PXC) based on one skating (including an agility parcours) and one classical distance competition.

Results

The key findings of this research were: 1) Age and maturity status were associated to boys'and girls' PXC; 2) Significant moderate to high correlations between girls' and boys' short duration XC skiing sprint performance 30-50 m (double poling (DP) flat and uphill, free skating, leg skating and V1 uphill skating) and PXC were revealed; 3) In general, the best prediction for PXC (Boys and Girls) was found to be the asymmetrical uphill (V1 40 m uphill) sub-technique; and 50 m DP (flat) while Boys' PXC was determined by V1 skating and girls' performance mainly by 50 m free skating (flat); 4) When using maturity offset as a confounding variable, boys' and girls' PXC was still highly associated with short duration skiing tests.

Discussion

In conclusion, the use of simple, non-invasive XC skiing sprint tests for evaluating PXC can be beneficial for ski clubs, specialized schools, or skiing federations in identifying and training young talented skiers. Further, this result demonstrates that skiing abilities such as short duration maximal speed and the proper use of different sub-techniques at high speeds during XC skiing is an important performance prerequisite.
Effects of rare earth as feed additive on production performance, egg quality, serum biochemical parameters, antioxidant capacity, intestinal morphology, and gut microbiota in late-phase laying hens
Authors: Li Jiaming , Yan Liwen , Cao Xuehai , Luo Yueming , Peng Xintong , Wang Zirui , Zou Tiande , Chen Jun , You Jinming
Published: April 2023

This study aimed to investigate the effects of dietary rare earth (RE) supplementation on production performance, egg quality, serum biochemical parameters, antioxidant capacity, intestinal morphology, and gut microbiota in late-phase laying hens. A total of 960 Lohmann Pink laying hens (380 d old) were randomly assigned to 1 of 5 dietary treatments in a 21-day feeding trial. There were 6 replicates in each treatment, with 32 hens per replicate. The five experimental diets were supplemented with 0, 150, 300, 450, and 600 g/t RE in the basal diet. Compared with the control group, hens fed the 150 g/t RE diet had a greater average egg weight during the third week of the experimental period (p < 0.05). However, dietary 150, 300, or 600 g/t RE supplementation decreased the eggshell thickness of laying hens compared to that of the control group (p < 0.05). No differences were observed in the serum biochemical parameters of laying hens among treatments except for the HDL-C concentration, which was higher in the 300 or 450 g/t RE-supplemented group than in the control group (p < 0.05). However, GSH-Px activity increased when hens were fed the 600 vs. 0 g/t RE diet (p < 0.05). But dietary supplementation with 600 g/t RE increased the ileum’s crypt depth in laying hens compared to the control group (p < 0.05). There were significant differences in beta diversity of cecum microbiota in laying hens fed a 600 g/t RE diet in place of the other 4 experimental diets (p < 0.05). Compared with the control diet, dietary 600 g/t RE supplementation significantly decreased the relative abundance of Fusobacteriota (phylum) and Fusobacterium (genus) while markedly increasing the relative abundance of Ruminococcus (genus) and Subdoligranulum (genus) (p < 0.05). A high RE dosage negatively affects egg quality and intestinal morphology and alters gut microbiota diversity and composition. In contrast, a moderate RE dosage has beneficial effects on production performance in late-phase laying hens. Further research is warranted regarding eggshell thickness to investigate whether dietary calcium levels must be adjusted when 150 g/t RE is supplemented for late-phase laying hens.
Keeping the Essentials in Place: Lessons Learned from a Qualitative Study of DREAMS in Northern Uganda
Authors: Gardsbane Diane , Bukuluki Paul
Published: April 2023

Peer-facilitated curriculum-based programs, including Stepping Stones, have been shown to be effective in preventing HIV and reducing gender-based violence (GBV). We conducted a qualitative study in early 2017 to hear perspectives of adolescent girls and young women (AGYW) participating in the USAID-funded Determined, Resilient, Empowered, AIDS-Free, Mentored, and Safe Women (DREAMS) intervention (administered by the President’s Emergency Fund for AIDS Relief (PEPFAR) in northern Uganda that featured 10 peer-facilitated sessions of a Stepping Stones curriculum. The study focused on asking AGYW how the initiative had affected their lives and on identifying lessons learned that could support future initiatives. A total of 56 AGYW were interviewed, including the peer facilitator and 6–7 randomly selected participants of nine DREAMS groups in Northern Uganda. Overwhelmingly, participants indicated that regular HIV testing and knowing their status, knowledge and an increased use of family planning, and knowing how to respond to GBV were among the results of their participation. However, a problematic finding was that peer group discussions relating to reducing GBV included advising AGYW about how to adjust their own behavior in ways that would reduce tension with their male partners, rather than shifting harmful gender norms. This is not consistent with the Stepping Stones program and prompted a retrospective review of factors related to how the program was implemented to better understand this result. Our study points to the important role facilitators play in shifting challenging gender norms, and the importance of fidelity to original program designs, as well as appropriate adaptations for different contexts. Our findings also signal the need for funders to allow sufficient time to pilot and adapt models.
An Evaluation of Fixed-Wing Unmanned Aerial Vehicle Trends and Correlations with Respect to NATO Classification, Region, EIS Date and Operational Specifications
Authors: Mitridis Dimitrios , Kapsalis Stavros , Terzis Dimitrios , Panagiotou Pericles
Published: April 2023

The current study provides a thorough analysis and evaluation of fixed-wing UAV correlations with respect to NATO classification, region of production, entry-into-service (EIS) date and other operational specifications. A set of 202 existing fixed-wing platforms is used to populate an in-house database. A screening of the corresponding data is conducted using a correlations matrix, and a statistical analysis of the key UAV design parameters is, in turn, performed. The results are presented using a wide variety of charts and statistical coefficients, to provide as much information as possible for future UAV design and performance assessment studies. Correlations for each mission type are provided, followed by a detailed evaluation of the key design parameters and design ratios (wingspan, gross takeoff weight, empty weight, payload weight, endurance, and operational speeds) with respect to NATO classification and region of origin. These key parameters are then plotted as a function of EIS date for every NATO category to identify any underlying trends and, finally, the platforms are classified in regard to some qualitative attributes, such as mission type and low observability. The results suggest that the trendlines extracted for each category significantly deviate from the generic trends. Therefore, omitting the classification in terms of region, size and weight can lead to misleading outcomes and should be avoided. Another conclusion lies in the fact that, apart from the average trendline, the design engineers should also have an indication of the data variance, due to the high dispersion observed in the datasets of several design parameters.
Investigation of Very Large Eddy Simulation Method for Applications of Supersonic Turbulent Combustion
Authors: Yan Chong , Xu Yibing , Cao Ruizhe , Piao Ying
Published: April 2023

The very large eddy simulation (VLES) method was investigated for supersonic reacting flows in the present work. The advantages and characteristics of the VLES model and the widely used improved delayed detached eddy simulation (IDDES) method were revealed through a supersonic ramped-cavity cold flow. Compared to the IDDES model, the VLES model transformed from RANS mode to LES mode faster, resulting in a smaller gray region caused by the mode transition. However, the original volume-averaging truncation length scale could lead to poor predictions of the velocity profiles and wall pressure distribution. By introducing a hybrid truncation length scale combining the maximum grid length and the shear layer adaptive (SLA) length with different coefficients, the accuracy of the VLES method was significantly improved, and the issue of the low shear layer position was solved. Moreover, owing to the resolution control function, the VLES method could adaptively model more turbulent kinetic energy and maintain a good accuracy in a coarser mesh. Finally, the modified VLES method was applied in conjunction with a hybrid combustion model constructed by the partially stirred reactor (PaSR) model and the Ingenito supersonic combustion model (ISCM) in simulations of the supersonic flame in the DLR scramjet combustor. After introducing the correction of the molecular collision frequency by the ISCM, the results obtained by the hybrid combustion model were more consistent with the experimental results, especially for the time-averaging temperature profile in the ignition zone.
Feasibility Study of the Bare-Photovoltaic-Tether Concept: Prototypes and Experimental Performance Evaluation of the Photovoltaic Tether Segment
Authors: Peiffer Leo , Perfler Christian , Tajmar Martin
Published: April 2023

Consumable-free electron emitters are presently not feasible for autonomous tether-based deorbit devices such as E.T.PACK due to their power requirement. The bare-photovoltaic-tether (BPT) concept combines the bare tether electron collection with a tether segment, coated with thin film Copper Indium Gallium Selenide solar cells to harvest additional power for the cathodic contact, potentially enabling propellant-less operation. This thesis presents the first prototype of the photovoltaic tether segment, its architecture, its electrical characteristics, major challenges of the system and possible solutions. Photovoltaic tether segments of up to 3 m in length were manufactured, consisting of parallelized submodules of 25 cm in length. Due to space limitations, only the I-V-characteristics of these submodules were measured under a self-built Class BCA LED Solar-Simulator inside a vacuum chamber and at varying temperatures between −100 °C and 100 °C. In addition, the suitability of the concept for a low Earth orbit environment was assessed by performing atomic oxygen exposure tests using a microwave-based low pressure plasma atomic oxygen source. Based on the experimental data, a model is provided for predicting the performance of the photovoltaic segment in orbit, highlighting the main problems of the BPT: temperature, orientation and partial shading.
Schizophrenia Synaptic Pathology and Antipsychotic Treatment in the Framework of Oxidative and Mitochondrial Dysfunction: Translational Highlights for the Clinics and Treatment
Authors: De Simone Giuseppe , Mazza Benedetta , Vellucci Licia , Barone Annarita , Ciccarelli Mariateresa , de Bartolomeis Andrea
Published: April 2023

Schizophrenia is a worldwide mental illness characterized by alterations at dopaminergic and glutamatergic synapses resulting in global dysconnectivity within and between brain networks. Impairments in inflammatory processes, mitochondrial functions, energy expenditure, and oxidative stress have been extensively associated with schizophrenia pathophysiology. Antipsychotics, the mainstay of schizophrenia pharmacological treatment and all sharing the common feature of dopamine D2 receptor occupancy, may affect antioxidant pathways as well as mitochondrial protein levels and gene expression. Here, we systematically reviewed the available evidence on antioxidants’ mechanisms in antipsychotic action and the impact of first- and second-generation compounds on mitochondrial functions and oxidative stress. We further focused on clinical trials addressing the efficacy and tolerability of antioxidants as an augmentation strategy of antipsychotic treatment. EMBASE, Scopus, and Medline/PubMed databases were interrogated. The selection process was conducted in respect of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. Several mitochondrial proteins involved in cell viability, energy metabolism, and regulation of oxidative systems were reported to be significantly modified by antipsychotic treatment with differences between first- and second-generation drugs. Finally, antioxidants may affect cognitive and psychotic symptoms in patients with schizophrenia, and although the evidence is only preliminary, the results indicate that further studies are warranted.
New Indole-3-Propionic Acid and 5-Methoxy-Indole Carboxylic Acid Derived Hydrazone Hybrids as Multifunctional Neuroprotectors
Authors: Anastassova Neda , Stefanova Denitsa , Hristova-Avakumova Nadya , Georgieva Irina , Kondeva-Burdina Magdalena , Rangelov Miroslav , Todorova Nadezhda , Tzoneva Rumiana , Yancheva Denitsa
Published: April 2023

In light of the known neuroprotective properties of indole compounds and the promising potential of hydrazone derivatives, two series of aldehyde-heterocyclic hybrids combining those pharmacophores were synthesized as new multifunctional neuroprotectors. The obtained derivatives of indole-3-propionic acid (IPA) and 5-methoxy-indole carboxylic acid (5MICA) had good safety profiles: Hemolytic effects < 5% (200 μM) and IC50 > 150 µM were found in the majority of the SH-SY5Y and bEnd3 cell lines. The 2,3-dihydroxy, 2-hydroxy-4-methoxy, and syringaldehyde derivatives of 5MICA exhibited the strongest neuroprotection against H2O2-induced oxidative stress in SH-SY5Y cells and 6-OHDA-induced neurotoxicity in rat-brain synaptosomes. All the compounds suppressed the iron-induced lipid peroxidation. The hydroxyl derivatives were also the most active in terms of deoxyribose-degradation inhibition, whereas the 3,4-dihydroxy derivatives were able to decrease the superoxide-anion generation. Both series of compounds showed an increased inhibition of hMAO-B, with greater expression detected in the 5MICA hybrids. The in vitro BBB model with the bEnd3 cell line showed that some compounds increased the permeability of the endothelial monolayer while maintaining the tight junctions. The combined results demonstrated that the derivatives of IPA and 5MICA showed strong neuroprotective, antioxidant, MAO-B inhibitory activity and could be considered as prospective multifunctional compounds for the treatment of neurodegenerative disorders.
5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy
Authors: Howley Richard , Chandratre Sharayu , Chen Bin
Published: April 2023

5-Aminolevulinic acid (ALA) is a naturally occurring amino acid synthesized in all nucleated mammalian cells. As a porphyrin precursor, ALA is metabolized in the heme biosynthetic pathway to produce protoporphyrin IX (PpIX), a fluorophore and photosensitizing agent. ALA administered exogenously bypasses the rate-limit step in the pathway, resulting in PpIX accumulation in tumor tissues. Such tumor-selective PpIX disposition following ALA administration has been exploited for tumor fluorescence diagnosis and photodynamic therapy (PDT) with much success. Five ALA-based drugs have now received worldwide approval and are being used for managing very common human (pre)cancerous diseases such as actinic keratosis and basal cell carcinoma or guiding the surgery of bladder cancer and high-grade gliomas, making it the most successful drug discovery and development endeavor in PDT and photodiagnosis. The potential of ALA-induced PpIX as a fluorescent theranostic agent is, however, yet to be fully fulfilled. In this review, we would like to describe the heme biosynthesis pathway in which PpIX is produced from ALA and its derivatives, summarize current clinical applications of ALA-based drugs, and discuss strategies for enhancing ALA-induced PpIX fluorescence and PDT response. Our goal is two-fold: to highlight the successes of ALA-based drugs in clinical practice, and to stimulate the multidisciplinary collaboration that has brought the current success and will continue to usher in more landmark advances.
Bipedal Static Supination and Dynamic Forefoot Loading Characteristics in Taiwanese College Badminton Players: A Cross-Sectional Study
Authors: Chow Tong-Hsien , Hsu Chin-Chia , Chen Chih-Cheng , Hsu Chin-Hsien
Published: April 2023

Context: Badminton is a unilateral sport that involves repetitive jumping, lunging and quick changes of direction with the lower limb, thus, plantar pressure profiles and foot postural profiles are critical to maintaining balance and coordination. Objective: The purpose of this study was to explore the characteristics of static and dynamic plantar pressure profiles with rearfoot posture in elite and recreational badminton players as well as assess the transitional changes of plantar loads between static and dynamic states. Methods: A cross-sectional survey was conducted among 65 college-level elite male badminton players (mean age: 20.2 ± 1.2 years; mean height: 177.4 ± 4.6 cm; mean weight: 72.6 ± 4.6 kg) and 68 recreational badminton players of the same gender (mean age: 19.9 ± 0.8 years; mean height: 170.3 ± 3.9 cm; mean weight: 67.7 ± 3.2 kg). The JC Mat was used to evaluate the arch index (AI), plantar pressure distribution (PPD), centers of gravity, and the characteristics of the footprint. Static foot posture was determined by examining the rearfoot alignment. Results: Both groups’ AI fell within the normal range. The static plantar loads of the elite group were distributed at the bipedal lateral part of longitudinal arches and heels (p < 0.01), while the right foot experienced higher centers of gravity (p < 0.05). The elite group’s static rearfoot postural alignment exhibited a higher degree of rearfoot varus than the recreational group (p < 0.05). In addition, the elite group’s dynamic plantar loads were mainly exerted at the medial and lateral metatarsals of both feet (p < 0.05). During the transition state, the recreational group’s plantar loads were mainly shifted to the bipedal lateral part of metatarsals and heels (p < 0.05), whereas the elite group’s bipedal lateral longitudinal arches as well as the medial and lateral heels experienced a reduction in plantar loads (p < 0.01). Conclusion: For elite badminton players, the findings revealed a possible connection among the static supinated foot, centers of gravity tending towards the right foot, and increased forefoot plantar loads in the dynamic state. The finding merits further exploration of the possible links between transitional changes in plantar pressure distribution in both states and related foot injuries resulting from intense competition and regular training in badminton.
Biomechanical Investigation of Hallux Valgus Deformity Treated with Different Osteotomy Methods and Kirschner Wire Fixation Strategies Using the Finite Element Method
Authors: Shih Kao-Shang , Hsu Ching-Chi , Huang Guan-Ting
Published: April 2023

The aim of this study was to propose a finite element method based numerical approach for evaluating various hallux valgus treatment strategies. We developed three-dimensional hallux valgus deformity models, with different metatarsal osteotomy methods and Kirschner wire fixation strategies, under two types of standing postures. Ten Kirschner wire fixations were analyzed and compared. The fixation stability, bone stress, implant stress, and contact pressure on the osteotomy surface were calculated as the biomechanical indexes. The results showed that the biomechanical indexes of the osteotomy and Kirschner wire fixations for hallux valgus deformity could be effectively analyzed and fairly evaluated. The distal metatarsal osteotomy method provided better biomechanical indexes compared to the proximal metatarsal osteotomy method. This study proposed a finite element method based numerical approach for evaluating various osteotomy and Kirschner wire fixations for hallux valgus deformity before surgery.
Bioengineered Mesenchymal-Stromal-Cell-Derived Extracellular Vesicles as an Improved Drug Delivery System: Methods and Applications
Authors: Ulpiano Cristiana , da Silva Cláudia L. , Monteiro Gabriel A.
Published: April 2023

Extracellular vesicles (EVs) are cell-derived nano-sized lipid membranous structures that modulate cell–cell communication by transporting a variety of biologically active cellular components. The potential of EVs in delivering functional cargos to targeted cells, their capacity to cross biological barriers, as well as their high modification flexibility, make them promising drug delivery vehicles for cell-free therapies. Mesenchymal stromal cells (MSCs) are known for their great paracrine trophic activity, which is largely sustained by the secretion of EVs. MSC-derived EVs (MSC-EVs) retain important features of the parental cells and can be bioengineered to improve their therapeutic payload and target specificity, demonstrating increased therapeutic potential in numerous pre-clinical animal models, including in the treatment of cancer and several degenerative diseases. Here, we review the fundamentals of EV biology and the bioengineering strategies currently available to maximize the therapeutic value of EVs, focusing on their cargo and surface manipulation. Then, a comprehensive overview of the methods and applications of bioengineered MSC-EVs is presented, while discussing the technical hurdles yet to be addressed before their clinical translation as therapeutic agents.
The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients
Authors: Blatkiewicz Małgorzata , Kamiński Kacper , Szyszka Marta , Al-Shakarchi Zaid , Olechnowicz Anna , Stelcer Ewelina , Komarowska Hanna , Tyczewska Marianna , Klimont Anna , Karczewski Marek , Wierzbicki Tomasz , Mikołajczyk-Stecyna Joanna , Ruchała Marek , Malendowicz Ludwik K. , Ruciński Marcin
Published: April 2023

Zwilch kinetochore protein (ZWILCH) plays a key role in proper cell proliferation. The upregulation of the ZWILCH gene was observed in many types of cancers, but the association of ZWILCH with adrenocortical carcinoma (ACC) was not investigated so far. The main aim of the presented study was to verify if the enhanced level of the ZWILCH gene can be used as a diagnostic marker for ACC development and progression, as well as a predictor of survival time for ACC patients. The performed analyses included investigation of the ZWILCH expression profile in tumors with publicly available TCGA (The Cancer Genome Atlas) datasets and transcriptomic data from the Gene Expression Omnibus (GEO) database, as well as, in human biological samples of normal adrenal, adrenocortical carcinoma and in commercially available tissue microarrays. The findings demonstrate statistically significant higher ZWILCH gene expression in ACC tissue in comparison with normal adrenal glands. Furthermore, there is a strong correlation between ZWILCH upregulation and tumor mitotic rate and the probability of patient survival. The enhanced ZWILCH level is also connected with the activation of genes involved in cell proliferation and the inhibition of genes related to the immune system. This work contributes to a better understanding of the role of ZWILCH as an ACC biomarker and diagnostic tool.
High Fidelity Pressure Wires Provide Accurate Validation of Non-Invasive Central Blood Pressure and Pulse Wave Velocity Measurements
Authors: Scalia Alessandro , Ghafari Chadi , Navarre Wivine , Delmotte Philippe , Phillips Rob , Carlier Stéphane
Published: April 2023

Central blood pressure (cBP) is known to be a better predictor of the damage caused by hypertension in comparison with peripheral blood pressure. During cardiac catheterization, we measured cBP in the ascending aorta with a fluid-filled guiding catheter (FF) in 75 patients and with a high-fidelity micromanometer tipped wire (FFR) in 20 patients. The wire was withdrawn into the brachial artery and aorto-brachial pulse wave velocity (abPWV) was calculated from the length of the pullback and the time delay between the ascending aorta and the brachial artery pulse waves by gating to the R-wave of the ECG for both measurements. In 23 patients, a cuff was inflated around the calf and an aorta-tibial pulse wave velocity (atPWV) was calculated from the distance between the cuff around the leg and the axillary notch and the time delay between the ascending aorta and the tibial pulse waves. Brachial BP was measured non-invasively and cBP was estimated using a new suprasystolic oscillometric technology. The mean differences between invasively measured cBP by FFR and non-invasive estimation were −0.4 ± 5.7 mmHg and by FF 5.4 ± 9.4 mmHg in 52 patients. Diastolic and mean cBP were both overestimated by oscillometry, with mean differences of −8.9 ± 5.5 mmHg and −6.4 ± 5.1 mmHg compared with the FFR and −10.6 ± 6.3 mmHg and −5.9 ± 6.2 mmHg with the FF. Non-invasive systolic cBP compared accurately with the high-fidelity FFR measurements, demonstrating a low bias (≤5 mmHg) and high precision (SD ≤ 8 mmHg). These criteria were not met when using the FF measurements. Invasively derived average Ao-brachial abPWV was 7.0 ± 1.4 m/s and that of Ao-tibial atPWV was 9.1 ± 1.8 m/s. Non-invasively estimated PWV based on the reflected wave transit time did not correlate with abPWV or with atPWV. In conclusion, we demonstrate the advantages of a novel method of validation for non-invasive cBP monitoring devices using acknowledged gold standard FFR wire transducers and the possibility to easily measure PWV during coronary angiography with the impact of cardiovascular risk factors.
Biomarkers of Tumor Heterogeneity in Glioblastoma Multiforme Cohort of TCGA
Authors: Winkelmaier Garrett , Koch Brandon , Bogardus Skylar , Borowsky Alexander D. , Parvin Bahram
Published: April 2023

Tumor Whole Slide Images (WSI) are often heterogeneous, which hinders the discovery of biomarkers in the presence of confounding clinical factors. In this study, we present a pipeline for identifying biomarkers from the Glioblastoma Multiforme (GBM) cohort of WSIs from TCGA archive. The GBM cohort endures many technical artifacts while the discovery of GBM biomarkers is challenged because “age” is the single most confounding factor for predicting outcomes. The proposed approach relies on interpretable features (e.g., nuclear morphometric indices), effective similarity metrics for heterogeneity analysis, and robust statistics for identifying biomarkers. The pipeline first removes artifacts (e.g., pen marks) and partitions each WSI into patches for nuclear segmentation via an extended U-Net for subsequent quantitative representation. Given the variations in fixation and staining that can artificially modulate hematoxylin optical density (HOD), we extended Navab’s Lab method to normalize images and reduce the impact of batch effects. The heterogeneity of each WSI is then represented either as probability density functions (PDF) per patient or as the composition of a dictionary predicted from the entire cohort of WSIs. For PDF- or dictionary-based methods, morphometric subtypes are constructed based on distances computed from optimal transport and linkage analysis or consensus clustering with Euclidean distances, respectively. For each inferred subtype, Kaplan–Meier and/or the Cox regression model are used to regress the survival time. Since age is the single most important confounder for predicting survival in GBM and there is an observed violation of the proportionality assumption in the Cox model, we use both age and age-squared coupled with the Likelihood ratio test and forest plots for evaluating competing statistics. Next, the PDF- and dictionary-based methods are combined to identify biomarkers that are predictive of survival. The combined model has the advantage of integrating global (e.g., cohort scale) and local (e.g., patient scale) attributes of morphometric heterogeneity, coupled with robust statistics, to reveal stable biomarkers. The results indicate that, after normalization of the GBM cohort, mean HOD, eccentricity, and cellularity are predictive of survival. Finally, we also stratified the GBM cohort as a function of EGFR expression and published genomic subtypes to reveal genomic-dependent morphometric biomarkers.
Analysis of Local Recurrence Risk in Ductal Carcinoma In Situ and External Validation of the Memorial Sloan Kettering Cancer Center Nomogram
Authors: Oses Gabriela , Mension Eduard , Pumarola Claudia , Castillo Helena , Francesc León , Torras Inés , Cebrecos Isaac , Caparrós Xavier , Ganau Sergi , Ubeda Belén , Bargallo Xavier , González Blanca , Sanfeliu Esther , Vidal-Sicart Sergi , Moreno Reinaldo , Muñoz Montserrat , Santamaría Gorane , Mollà Meritxell
Published: April 2023

Background: Adjuvant radiotherapy and hormonotherapy after breast-conserving surgery (BCS) in ductal carcinoma in situ (DCIS) have been shown to reduce the risk of local recurrence. To predict the risk of ipsilateral breast tumor relapse (IBTR) after BCS, the Memorial Sloan Kettering Cancer Center (MSKCC) developed a nomogram to analyze local recurrence (LR) risk in our cohort and to assess its external validation. Methods: A historical cohort study using data from 296 patients treated for DCIS at the Hospital Clínic of Barcelona was carried out. Patients who had had a mastectomy were excluded from the analysis. Results: The mean age was 58 years (42–75), and the median follow-up time was 10.64 years. The overall local relapse rate was 13.04% (27 patients) during the study period. Actuarial 5- and 10-year IBTR rates were 5.8 and 12.9%, respectively. The external validation of the MSKCC nomogram was performed using a multivariate logistic regression analysis on a total of 207 patients, which did not reach statistical significance in the studied population for predicting LR (p = 0.10). The expression of estrogen receptors was significantly associated with a decreased risk of LR (OR: 0.25; p = 0.004). Conclusions: In our series, the LR rate was 13.4%, which was in accordance with the published series. The MSKCC nomogram did not accurately predict the IBTR in this Spanish cohort of patients treated for DCIS (p = 0.10).
Advances in a Microwave Sensor-Type Interdigital Capacitor with a Hexagonal Complementary Split-Ring Resonator for Glucose Level Measurement
Authors: Harnsoongnoen Supakorn , Buranrat Benjaporn
Published: April 2023

This study involved the creation and assessment of a microwave sensor to measure glucose levels in aqueous solutions without invasiveness. The sensor design utilized a planar interdigital capacitor (IDC) loaded with a hexagonal complementary split-ring resonator (HCSRR). The HCSRR was chosen for its ability to generate a highly intense electric field that is capable of detecting variations in the dielectric characteristics of the specimen. A chamber tube was used to fill glucose solutions at the sensor’s sensitive area, and changes in the device’s resonance frequency (Fr) and reflection coefficient (S11) were used to measure glucose levels. Fitting formulas were developed to analyze the data, and laboratory tests showed that the sensor could accurately measure glucose levels within a range of 0–150 mg/dL. At a concentration of 37.5 mg/dL, the sensitivity based on S11 and Fr reached maximum values of 10.023 dB per mg/dL and 1.73 MHz per mg/dL, respectively. This implies that the sensor put forward has the possibility of being utilized in medical settings for the monitoring of glucose levels.
MXene/NiO Composites for Chemiresistive-Type Room Temperature Formaldehyde Sensor
Authors: Huang Baoyu , Tong Xinwei , Zhang Xiangpeng , Feng Qiuxia , Rumyantseva Marina N. , Prakash Jai , Li Xiaogan
Published: April 2023

In this work, MXene/NiO-composite-based formaldehyde (HCHO) sensing materials were successfully synthesized by an in situ precipitation method. The heterostructures between the MXene and NiO nanoparticles were verified by transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The HCHO sensing performance of the MXene/NiO-based chemiresistive-type sensors was investigated. Compared to pure MXene and NiO materials, the sensing performance of the MXene/NiO-P2-based sensor to HCHO gas at room temperature was significantly enhanced by the formation of MXene/NiO heterojunctions. The response of the MXene/NiO-P2 sensor to 50 ppm HCHO gas was 8.8, which was much higher than that of the pure MXene and NiO. At room temperature, the detectable HCHO concentration of the MXene/NiO-P2-based sensor was 1 ppm, and the response and recovery time to 2 ppm HCHO was 279 s and 346 s, respectively. The MXene/NiO-P2 sensor also exhibited a good selectivity and a long-term stability to HCHO gas for 56 days. The in situ Fourier transform infrared (FTIR) spectra of the MXene/NiO-P2 sensor, when exposed to HCHO gas at different times, were investigated to verify the adsorption reaction products of HCHO molecules.
Enriching Athlete—Environment Interactions in Youth Sport: The Role of a Department of Methodology
Authors: Davids Keith , Rothwell Martyn , Hydes Sam , Robinson Tim , Davids Charlie
Published: April 2023

The aim of this insights paper is to propose how the theory of ecological dynamics may invite re-consideration of how sport scientists could support performance, learning and development of children and youth in sports programmes. We seek to outline why learning should be individualised and contextualised, based on the specific needs of learners, such as children and youth, women and disabled athletes in sport. Case examples from individual and team sports are presented to illustrate how constraints can be designed to enrich interactions of children and youth with different performance environments, based on integrating principles of specificity and generality in learning and development. These case examples suggest how a collaborative effort by sport scientists and coaches in children and youth sport may be undertaken in a department of methodology to enrich learning and performance.
Prevalence of Dental Anomalies and Its Role in Sex Estimation among Children of Jazan Region, Saudi Arabia
Authors: Renugalakshmi Apathsakayan , Vinothkumar Thilla Sekar , Bokhari Ahmed M. , Almahdi Samaher , Almalki Abdulrahman , Balla Sudheer Babu , Tadakamadla Santosh Kumar , Hakami Zaki
Published: April 2023

Background: This study aimed to ascertain the prevalence of dental anomalies and their ability to estimate sex status. Material and Methods: This cross-sectional radiographic study was based on the evaluation of dental anomalies of Saudi children aged between 5 and 17 years. A total of 1940 orthopantomograms (OPG) were screened, of which 1442 were included. All the OPGs were digitally evaluated with ImageJ software. The demographic variables and dental anomaly findings were subjected to descriptive and comparative statistical analysis. Discriminant function analysis was conducted for sex estimation. p value < 0.05 was considered as significant. Results: The mean age of the children in this study was 11.35 ± 0.28 years. At least one dental anomaly was detected in 161 children (11.17%), including 71 males and 90 females. Only 13 children (8.07%) presented with more than one anomaly. The most common dental anomaly detected was root dilaceration (47.83%) followed by hypodontia (31.68%). The least common dental anomaly was infraocclusion (1.86%). The sex prediction accuracy using discriminant function analysis was 62.9% (p < 0.01). Conclusion: The prevalence of dental anomalies was 11.17% with root dilaceration and hypodontia being the most common. The role of dental anomalies in sex estimation was found to be ineffective.
Plant Antimicrobial Peptides: Insights into Structure-Function Relationships for Practical Applications
Authors: Slezina Marina P. , Odintsova Tatyana I.
Published: April 2023

Antimicrobial peptides (AMPs) are short polypeptide molecules produced by multicellular organisms that are involved in host defense and microbiome preservation. In recent years, AMPs have attracted attention as novel drug candidates. However, their successful use requires detailed knowledge of the mode of action and identification of the determinants of biological activity. In this review, we focused on structure-function relationships in the thionins, α-hairpinins, hevein-like peptides, and the unique Ib-AMP peptides isolated from Impatiens balsamina. We summarized the available data on the amino acid sequences and 3D structure of peptides, their biosynthesis, and their biological activity. Special attention was paid to the determination of residues that play a key role in the activity and the identification of the minimal active cores. We have shown that even subtle changes in amino acid sequences can affect the biological activity of AMPs, which opens up the possibility of creating molecules with improved properties, better therapeutic efficacy, and cheaper large-scale production.
Using AlphaFold Predictions in Viral Research
Authors: Gutnik Daria , Evseev Peter , Miroshnikov Konstantin , Shneider Mikhail
Published: April 2023

Elucidation of the tertiary structure of proteins is an important task for biological and medical studies. AlphaFold, a modern deep-learning algorithm, enables the prediction of protein structure to a high level of accuracy. It has been applied in numerous studies in various areas of biology and medicine. Viruses are biological entities infecting eukaryotic and procaryotic organisms. They can pose a danger for humans and economically significant animals and plants, but they can also be useful for biological control, suppressing populations of pests and pathogens. AlphaFold can be used for studies of molecular mechanisms of viral infection to facilitate several activities, including drug design. Computational prediction and analysis of the structure of bacteriophage receptor-binding proteins can contribute to more efficient phage therapy. In addition, AlphaFold predictions can be used for the discovery of enzymes of bacteriophage origin that are able to degrade the cell wall of bacterial pathogens. The use of AlphaFold can assist fundamental viral research, including evolutionary studies. The ongoing development and improvement of AlphaFold can ensure that its contribution to the study of viral proteins will be significant in the future.