bluuebunny/crossref_metadata_2025 huggingface dataset:
Nature-Based Physical Activity and Hedonic and Eudaimonic Wellbeing: The Mediating Roles of Motivational Quality and Nature Relatedness
Authors: Jenkins Matthew , Lee Craig , Houge Mackenzie Susan , Hargreaves Elaine Anne , Hodge Ken , Calverley Jessica
Published: January 2022

The current study evaluated the degree to which nature-based physical activity (NPA) influenced two distinct types of psychological wellbeing: hedonic wellbeing and eudaimonic wellbeing. The type of motivation an individual experiences for physical activity, and the extent to which individuals have a sense of relatedness with nature, have been shown to influence the specific type of psychological wellbeing that is experienced as a result of NPA. However, the role of these two variables in the relationship between NPA and psychological wellbeing has not been examined. Thus, this study assessed the potential mediating influence of (1) motivational quality and (2) nature relatedness on the relationships between NPA and hedonic and eudaimonic wellbeing, respectively. Participants (N = 262) completed an online survey assessing hedonic and eudaimonic wellbeing, NPA, intrinsic motivation, autonomous extrinsic motivation, and nature relatedness. Data were analysed using Partial Least Squares Structural Equation Modelling. Results showed that motivational quality and nature relatedness both fully mediated the relationships between NPA and hedonic and eudaimonic wellbeing. Specifically, intrinsic motivation positively mediated the relationship between NPA and hedonic wellbeing. Autonomous extrinsic motivation and nature relatedness positively mediated the relationship between NPA and eudaimonic wellbeing. These findings suggest that the associations between NPA and eudaimonic wellbeing and hedonic wellbeing, respectively, are driven by different mechanisms relating to an individual’s (1) underlying motivation and (2) sense of connection to nature. These findings suggest that promoting distinct types of wellbeing (hedonic vs. eudaimonic) through NPA requires distinct approaches. Emphasising enjoyment, pleasure, and positive kinaesthetic experiences within NPA may be more conducive to hedonic wellbeing, while highlighting opportunities for connecting with nature or experiencing valued outcomes of NPA may be more conducive to eudaimonic wellbeing.
Suffering a Healthy Life—On the Existential Dimension of Health
Authors: Binder Per-Einar
Published: January 2022

This paper examines the existential context of physical and mental health. Hans Georg Gadamer and The World Health Organization’s conceptualizations are discussed, and current medicalized and idealized views on health are critically examined. The existential dimension of health is explored in the light of theories of selfhood consisting of different parts, Irvin Yalom’s approach to “ultimate concerns” and Martin Heidegger’s conceptualization of “existentials.” We often become aware of health as an existential concern during times of illness, and health and illness can co-exist. The paper discusses how existential suffering in Western culture is described, to an increasing degree, as disorders or psychological deficits, and perfectionistic health goals easily can become a problem. We seek to avoid suffering rather than relate to it, with all the tension that may create. The paper argues that suffering is an unavoidable aspect of people’s experience of their lives, and actively relating to suffering must be regarded as a fundamental aspect of health. The need and usefulness of a concept of “existential health” is discussed.
Neuroticism as Mediator and Moderator Between War Atrocities and Psychopathology in Syrian Refugee Children and Adolescents
Authors: Khamis Vivian
Published: January 2022

Background

Despite the extensive research on war atrocities and risk factors for psychopathology, there is a paucity of research on the potential mediating and moderating effect of neuroticism in refugee children and adolescents.

Objective

This study aimed to analyze whether neuroticism mediated and/or moderated the relationship between war atrocities and different types of psychopathology in Syrian refugee children and adolescents who resettled in Lebanon and Jordan.

Participants and Setting

Participants were 1,000 Syrian refugee children and adolescents of both sexes.

Methods

Questionnaires were administered in an interview format with children at school by two trained psychologists. Descriptive statistics and inter-correlations among variables were used Then the mediator and moderator effect of neuroticism in the relationship between war atrocities and posttraumatic stress, emotion regulation and behavioral and emotional disorders were examined.

Results

A partial mediating effect of neuroticism on posttraumatic stress disorder (PTSD), emotion dysregulation, and emotional and behavioral disorders was revealed. Findings also indicated full moderating effects of neuroticism on PTSD as well as partial moderating effects on emotion dysregulation, and emotional and behavioral.

Conclusions

Findings contribute to the neuroticism literature by showing that high-N children develop more problems that are psychopathological and have more severe affective reactions to war atrocities in post- resettlement contexts. Early interventions aimed to reduce neuroticism might contribute to a better prognosis in refugee children at high-risk for psychological disorders.
A Scoping Review of Interventions Designed to Support Parents With Mental Illness That Would Be Appropriate for Parents With Psychosis
Authors: Radley Jessica , Sivarajah Nithura , Moltrecht Bettina , Klampe Marie-Louise , Hudson Felicity , Delahay Rachel , Barlow Jane , Johns Louise C.
Published: January 2022

The experience of psychosis can present additional difficulties for parents, over and above the normal challenges of parenting. Although there is evidence about parenting interventions specifically targeted at parents with affective disorders, anxiety, and borderline personality disorder, there is currently limited evidence for parents with psychotic disorders. It is not yet known what, if any, interventions exist for this population, or what kinds of evaluations have been conducted. To address this, we conducted a scoping review to determine (1) what parenting interventions have been developed for parents with psychosis (either specifically for, or accessible by, this client group), (2) what components these interventions contain, and (3) what kinds of evaluations have been conducted. The eligibility criteria were broad; we included any report of an intervention for parents with a mental health diagnosis, in which parents with psychosis were eligible to take part, that had been published within the last 20 years. Two reviewers screened reports and extracted the data from the included reports. Thirty-eight studies of 34 interventions were included. The findings show that most interventions have been designed either for parents with any mental illness or parents with severe mental illness, and only two interventions were trialed with a group of parents with psychosis. After noting clusters of intervention components, five groups were formed focused on: (1) talking about parental mental illness, (2) improving parenting skills, (3) long-term tailored support for the whole family, (4) groups for parents with mental illness, and (5) family therapy. Twenty-three quantitative evaluations and 13 qualitative evaluations had been conducted but only eight interventions have or are being evaluated using a randomized controlled trial (RCT). More RCTs of these interventions are needed, in addition to further analysis of the components that are the most effective in changing outcomes for both the parent and their children, in order to support parents with psychosis and their families.
Resilience and Mental Well-Being During the COVID-19 Pandemic: Serial Mediation by Persistent Thinking and Anxiety About Coronavirus
Authors: Skalski Sebastian B. , Konaszewski Karol , Büssing Arndt , Surzykiewicz Janusz
Published: January 2022

Reports to date indicate that the COVID-19 pandemic outbreak has negatively impacted mental health in the general population. On the other hand, positive associations of mental resilience and well-being have been widely demonstrated. The objective of this study was to assess the links between resilience (Brief Resilience Scale), persistent thinking about COVID-19 (Obsession with COVID-19 Scale), coronavirus anxiety (Coronavirus Anxiety Scale), and well-being (World Health Organization's 5-item Well-being Index) using serial mediation. The study considered online survey data from 1,547 Poles aged 18–78 (62% of whom were women). Bootstrap sampling analysis revealed that persistent thinking about COVID-19 (M1) and coronavirus anxiety (M2) partially mediate the relationship between resilience and well-being. The results of this study indicate that persistent thinking may be dysfunctional for mental health, as it inflates pandemic anxiety and disrupts well-being. Moreover, practitioners should focus on interventions enhancing resilience in order to reduce negative mental effects during the spread of a pandemic infectious disease.
Research on Time-Driven Activity-Based Management System of Public Hospitals
Authors: Jiang Qiwen , Zhu Xueyuan , Chen Lianghua , Zhao Ziyuan , Chen Yilong
Published: January 2022

Objective

To provide references for effective implementing cost management for public hospitals through establishing time-driven activity-based management (TDABM) system. The TDABM system was established from hospital cost accounting, budget, control, and performance.

Results

The established TDABM system could improve the precision of hospital cost accounting, improve medical staff's working efficiency, realize the whole process of cost management, and enhance the competitiveness of the hospital.

Conclusion

The activity of implementing TDABM in public hospitals had practical significance.
Helping People Through Space and Time: Assistance as a Perspective on Human-Robot Interaction
Authors: Newman Benjamin A. , Aronson Reuben M. , Kitani Kris , Admoni Henny
Published: January 2022

As assistive robotics has expanded to many task domains, comparing assistive strategies among the varieties of research becomes increasingly difficult. To begin to unify the disparate domains into a more general theory of assistance, we present a definition of assistance, a survey of existing work, and three key design axes that occur in many domains and benefit from the examination of assistance as a whole. We first define an assistance perspective that focuses on understanding a robot that is in control of its actions but subordinate to a user’s goals. Next, we use this perspective to explore design axes that arise from the problem of assistance more generally and explore how these axes have comparable trade-offs across many domains. We investigate how the assistive robot handles other people in the interaction, how the robot design can operate in a variety of action spaces to enact similar goals, and how assistive robots can vary the timing of their actions relative to the user’s behavior. While these axes are by no means comprehensive, we propose them as useful tools for unifying assistance research across domains and as examples of how taking a broader perspective on assistance enables more cross-domain theorizing about assistance.
How Young Mothers Rely on Kin Networks and Formal Childcare to Avoid Becoming NEET in the Netherlands
Authors: Dicks Alexander , Levels Mark , van der Velden Rolf , Mills Melinda C.
Published: January 2022

Motherhood is often cited as one of the main reasons for young women to become NEET (not in employment, education, or training). Given the potential long-term negative implications of NEET status, it is important to understand which types of resources can help young mothers to avoid becoming NEET around childbirth. In this paper we investigate how the chances of young mothers to become and stay NEET around the time of first birth are related to the availability and characteristics of members of their social support network, especially partners and grandparents, to assist in childcare. In addition, we consider the local availability of formal childcare. We use population-wide register data from the Netherlands and estimate discrete-time eventhistory models. Our results show that young mothers who are cohabitating or married are less likely to become NEETs than single mothers. We also show that economic activity and relative wage of both young mothers and their partners decreases the likelihood to become NEET and to exit NEET. With respect to the grandparents, we find that having more grandparents live in the immediate vicinity is associated with a lower likelihood to become NEET and a higher likelihood to exit NEET. Furthermore, we find that young mothers with economically inactive parents are more likely to become and less likely to exit NEET. Lastly, we find evidence for crowding-out of informal and formal childcare. Formal and informal childcare sources interact in such a way that the role of either becomes less important as more of the other is available.
The Future of 3D Brain Cultures in Developmental Neurotoxicity Testing
Authors: Hogberg Helena T. , Smirnova Lena
Published: January 2022

Human brain is undoubtedly the most complex organ in the body. Thus, it is difficult to develop adequate and at the same time human relevant test systems and models to cover the aspects of brain homeostasis and even more challenging to address brain development. Animal tests for Developmental Neurotoxicity (DNT) have been devised, but because of complex underlying mechanisms of neural development, and interspecies differences, there are many limitations of animal-based approaches. The high costs, high number of animals used per test and technical difficulties of these tests are prohibitive for routine DNT chemical screening. Therefore, many potential DNT chemicals remain unidentified. New approach methodologies (NAMs) are needed to change this. Experts in the field have recommended the use of a battery of human in vitro tests to be used for the initial prioritization of high-risk environmental chemicals for DNT testing. Microphysiological systems (MPS) of the brain mimic the in vivo counterpart in terms of cellular composition, recapitulation of regional architecture and functionality. These systems amendable to use in a DNT test battery with promising features such as (i) complexity, (ii) closer recapitulation of in vivo response and (iii) possibility to multiplex many assays in one test system, which can increase throughput and predictivity for human health. The resent progress in 3D brain MPS research, advantages, limitations and future perspectives are discussed in this review.
Inflammatory Disease Affecting the Central Nervous System in Dogs: A Retrospective Study in England (2010–2019)
Authors: Gonçalves Rita , De Decker Steven , Walmsley Gemma , Butterfield Sarah , Maddox Thomas W.
Published: January 2022

The epidemiology of inflammatory diseases affecting the central nervous system (CNS) in dogs is largely unknown. We aimed to report the relative proportion of different causes of inflammatory disease affecting the CNS in dogs and identify predictors for infectious vs. immune-mediated conditions and predictors for the most common diseases affecting the brain and the spinal cord. This was a retrospective cohort study over a 10-year period in 2 referral institutions using multivariable and multinomial logistic regression for identification of risk factors. In total, 1,140 client-owned dogs diagnosed with inflammatory disease affecting the CNS were included. Fifteen different diagnoses were identified, with immune-mediated (83.6%) disease being more common than infectious conditions (16.4%). The most common immune-mediated conditions diagnosed were meningoencephalitis of unknown origin (47.5%) and steroid-responsive meningitis–arteritis (30.7%), and the most common infectious conditions were discospondylitis (9.3%) and otogenic intracranial infection (2.2%). Older age (p < 0.001, OR = 1.019, 95% CI: 1.014–1.024), higher body weight (p < 0.001, OR = 1.049, 95% CI: 1.025–1.074), male sex (p = 0.009, OR = 1.685, 95% CI: 1.141–2.488), longer duration of the clinical signs before presentation (p < 0.001, OR = 1.011, 95% CI: 1.006–1.017), progressive nature of the clinical signs (p < 0.001, OR = 2.295, 95% CI: 1.463–3.599), identification of a possibly associated preceding event (p = 0.0012, OR = 1.93, 95% CI: 1.159–3.213), and hyperesthesia on presentation (p < 0.001, OR = 2.303, 95% CI: 1.528–3.473) were associated with a diagnosis of infectious diseases. Our data shows that immune-mediated diseases are more common than infectious conditions as a cause for inflammatory CNS disease in dogs. The risk factors for the most common diagnoses were identified from signalment, history, and findings of the physical and neurological examinations to give valuable information that can guide clinicians with their investigations.
Acknowledgment to Reviewers of Atoms in 2021
Authors:   Atoms Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Acknowledgment to Reviewers of Audiology Research in 2021
Authors:   Audiology Research Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Acknowledgment to Reviewers of Conservation in 2021
Authors:   Conservation Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
A New Design of MP-HDCCB Topology Based on Hybrid Switching Device
Authors: Jia Haipeng , Yin Jingyuan , Wei Tongzhen , Li Jinke , Zhu Jin , Ye Zeyu
Published: January 2022

Since each branch of the multiterminal DC circuit system relies on the DC circuit breaker for breaking and fault isolation, the prohibitive cost and huge volume of the Hybrid DC Circuit Breaker (HCB) limit its development and broad application in multiterminal flexible DC systems. Multiport hybrid DC circuit breaker (MP-HDCCB) based on device and branch sharing reduces the configuration cost of the circuit breaker to a certain extent. In order to further reduce the cost of MP-HDCCB, a novel MP-HDCCB topology based on hybrid switching devices is proposed, adopting full controlled switching devices to achieve rapidity of breaking fault current, and using semi-controlled switching devices in series to withstand the transient interruption voltage (TIV), so as to reduce the construction cost and technical difficulty. In this paper, the working principle and fault breaking strategy of the topology are introduced in detail, then the parameters of the major circuit are analyzed theoretically, and the parameter design of each branch is given. In the end, the rationality and validity of the proposed topology is tested and verified by simulations and experimental tests.
Performance Analysis of a Double Pass Solar Air Thermal Collector with Porous Media Using Lava Rock
Authors: Ismail Amar Fahmi , Abd Hamid Ag Sufiyan , Ibrahim Adnan , Jarimi Hasila , Sopian Kamaruzzaman
Published: January 2022

This paper investigates double-pass solar air thermal collectors with lava rock as the porous media. The addition of lava rock serves as short-term sensible thermal storage for a solar drying system. It also enhances the convective heat transfer rate to the airflow due to an increased heat transfer area and increased turbulence in the air channel. A mathematical model was developed based on energy balance equations and was numerically solved in MATLAB. The collector’s thermal performance was studied at various levels of solar intensity and at different wind speeds for different design parameters: collector size, air mass flow rate, and lava rock volume. From the study, the optimum efficiencies that were obtained in the range between the intensities of 500 W/m2 and 800 W/m2 were 62% to 64%, respectively, with an optimum flow rate of 0.035 kg/s. The optimum porosity of about 89% was selected for the collector by considering the pressure drop and thermal efficiency. An optimal temperature output range between 41.7 °C and 48.3 °C could be achieved and was suitable for agricultural and food drying applications. Meanwhile, compared to conventional DPSAHs, the average percentage increase in the output temperature of the DPSAH with lava rock was found to be higher by 17.5%.
Acknowledgment to Reviewers of Epidemiologia in 2021
Authors:   Epidemiologia Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Pain and Avoidance during and after Endodontic Therapy: The Role of Pain Anticipation and Self-Efficacy
Authors: Santos-Puerta Noelia , Peñacoba-Puente Cecilia
Published: January 2022

Background: Pain anticipation has been identified as a predictor of pain and avoidance with respect to endodontic therapy. Self-efficacy is also key to the development and maintenance of health behaviors and achieve patient adherence to treatment. However, the role of self-efficacy has not been studied yet in endodontic treatment. Methods: This study was conducted on 101 patients who needed root canal therapy. They had to fill a questionnaire before treatment registered pain anticipation and self-efficacy; during and after treatment were registered pain intensity and avoidance. Results: Pain anticipation explained pain during (Beta = 0.51, t = 5.82, p ≤ 0.001, [0.34, 0.69]) and after treatment (Beta = 0.38, t = 4.35, p ≤ 0.001, [0.21, 0.55]). Self-efficacy did not have an influence in pain values. Pain anticipation explained avoidance during (Beta = 0.51, t = 3.60, p ≤ 0.001, [0.23, 0.80]) and after treatment (Beta = 0.62, t = 4.29, p ≤ 0.001, [0.33, 0.91]). Self-efficacy had a significant role in avoidance during treatment (Beta = 0.12, t = 2.19, p ≤ 0.03, [0.01, 0.23]) with a strong moderation relationship between pain anticipation and avoidance when self-efficacy was medium (Beta = 0.44, t = 3.24, p = 0.002, [0.17, 0.72]) or high (Beta = 0.84, t = 3.5, p ≤ 0.001, [0.37, 1.33]). Self-efficacy was not significant respect to avoidance after treatment. Conclusions: Self-efficacy is an important variable in endodontic therapy due to their moderating effect between pain anticipation and avoidance behavior during the procedure. It is necessary to improve the results of root canal therapy and reduce patient’s avoidance in order to take into account this variable.
Clinical Breakpoint of Apramycin to Swine Salmonella and Its Effect on Ileum Flora
Authors: Dai Xinyu , Gu Yufeng , Guo Jinli , Huang Lingli , Cheng Guyue , Peng Dapeng , Hao Haihong
Published: January 2022

The purpose of this study was to establish the clinical breakpoint (CBP) of apramycin (APR) against Salmonella in swine and evaluate its effect on intestinal microbiota. The CBP was established based on three cutoff values of wild-type cutoff value (COWT), pharmacokinetic-pharmadynamic (PK/PD) cutoff value (COPD) and clinical cutoff value (COCL). The effect of the optimized dose regimen based on ex vivo PK/PD study. The evolution of the ileum flora was determined by the 16rRNA gene sequencing and bioinformatics. This study firstly established the COWT, COPD in ileum, and COCL of APR against swine Salmonella, the value of these cutoffs were 32 µg/mL, 32 µg/mL and 8 µg/mL, respectively. According to the guiding principle of the Clinical Laboratory Standards Institute (CLSI), the final CBP in ileum was 32 µg/mL. Our results revealed the main evolution route in the composition of ileum microbiota of diarrheic piglets treated by APR. The change of the abundances of Bacteroidetes and Euryarchaeota was the most obvious during the evolution process. Methanobrevibacter, Prevotella, S24-7 and Ruminococcaceae were obtained as the highest abundance genus. The abundance of Methanobrevibacter increased significantly when APR treatment carried and decreased in cure and withdrawal period groups. The abundance of Prevotella in the tested groups was significantly lower than that in the healthy group. A decreased of abundance in S24-7 was observed after Salmonella infection and increased slightly after cure. Ruminococcaceae increased significantly after Salmonella infection and decreased significantly after APR treatment. In addition, the genera of Methanobrevibacter and Prevotella were defined as the key node. Valine, leucine and isoleucine biosynthesis, D-Glutamine and D-glutamate metabolism, D-Alanine metabolism, Peptidoglycan and amino acids biosynthesis were the top five Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in the ileum microbiota of piglets during the Salmonella infection and APR treatment process. Our study extended the understanding of dynamic shift of gut microbes during diarrheic piglets treated by APR.
Position Correction and Trajectory Optimization of Underwater Long-Distance Navigation Inspired by Sea Turtle Migration
Authors: Li Ziyuan , Yu Huapeng , Li Ye , Shen Tongsheng , Wang Chongyang , Cong Zheng
Published: January 2022

Accumulating evidence suggests that migrating animals store navigational “maps” in their brains, decoding location information from geomagnetic information based on their perception of the magnetic field. Inspired by this phenomenon, a novel geomagnetic inversion navigation framework was proposed to address the error constraint of a long-distance inertial navigation system. In the first part of the framework, the current paper proposed a geomagnetic bi-coordinate inversion localization approach which enables an autonomous underwater vehicle (AUV) to estimate its current position from geomagnetic information like migrating animals. This paper suggests that the combination of geomagnetic total intensity (F) and geomagnetic inclination (I) can determine a unique geographical location, and that there is a non-unique mapping relationship between the geomagnetic parameters and the geographical coordination (longitude and latitude). Then the cumulative error of the inertial navigation system is corrected, according to the roughly estimated position information. In the second part of the framework, a cantilever beam model is proposed to realize the optimal correction of the INS historical trajectory. Finally, the correctness of the geomagnetic bi-coordinate inversion localization model we proposed was verified by outdoor physical experiments. In addition, we also completed a geomagnetic/inertial navigation integrated long-distance semi-physical test based on the real navigation information of the AUV. The results show that the geomagnetic inversion navigation framework proposed in this paper can constrain long-distance inertial navigation errors and improve the navigation accuracy by 73.28% compared with the pure inertial navigation mode. This implies that the geomagnetic inversion localization will play a key role in long-distance AUV navigation correction.
Photocatalytic Reduction of Carbon Dioxide on TiO2 Heterojunction Photocatalysts—A Review
Authors: Barrocas Beatriz Trindade , Ambrožová Nela , Kočí Kamila
Published: January 2022

The photocatalytic reduction of carbon dioxide to renewable fuel or other valuable chemicals using solar energy is attracting the interest of researchers because of its great potential to offer a clean fuel alternative and solve global warming problems. Unfortunately, the efficiency of CO2 photocatalytic reduction remains not very high due to the fast recombination of photogenerated electron–hole and small light utilization. Consequently, tremendous efforts have been made to solve these problems, and one possible solution is the use of heterojunction photocatalysts. This review begins with the fundamental aspects of CO2 photocatalytic reduction and the fundamental principles of various heterojunction photocatalysts. In the following part, we discuss using TiO2 heterojunction photocatalysts with other semiconductors, such as C3N4, CeO2, CuO, CdS, MoS2, GaP, CaTiO3 and FeTiO3. Finally, a concise summary and presentation of perspectives in the field of heterojunction photocatalysts are provided. The review covers references in the years 2011–2021.
Probiotics Reduce Vaginal Candidiasis in Pregnant Women via Modulating Abundance of Candida and Lactobacillus in Vaginal and Cervicovaginal Regions
Authors: Ang Xin , Mageswaran Uma , Chung Yi , Lee Boon , Azhar Siti , Roslan Nurhanis , Saufian Ili , Mustaffa Nor , Kalam Ermadina , Ibrahim Aini , Abdul Wahid Normala , Deris Zakuan , Oon Chern-Ein , Adnan Wan , Sany Salina , Liong Min-Tze
Published: January 2022

We previously reported on the effects of a lactobacilli probiotic (SynForU-HerCare; two capsules/day of 9.5 log CFU/capsule) in improving symptoms of vaginal irritation, discharge and burning in pregnant women with vaginal candidiasis upon administration for 8 weeks, accompanied by improved emotional and social quality of life parameters. Thus, the present study aimed to analyse vaginal microbiota and inflammatory changes in hope to better understand the improved clinical symptoms as observed previously. Patients in the probiotic group showed a decreased abundance of Candida glabrata after 8 weeks (p = 0.009) in the lower vaginal region, while patients in the placebo group did not show any changes over time. In the higher vaginal and cervicovaginal regions, patients in the placebo group showed a decreased abundance of Candida albicans only within 4 weeks (p < 0.05) but no changes in abundance of C. glabrata over time, while patients in the probiotic group showed a continuous decreased abundance of C. albicans and C. glabrata over 8 weeks (p < 0.05). Patients in the placebo group also had a decreased abundance of Lactobacillus crispatus over 4 weeks (p = 0.023) in the lower vaginal region and a decreased abundance of L. jensenii over 8 weeks in the cervicovaginal region (p = 0.001). Meanwhile, patients in the probiotic group had an increased abundance of L. crispatus in the lower vaginal region after 8 weeks (p = 0.012) and Lactobacillus jensenii over 4 weeks in the cervicovaginal region (p < 0.001). Inflammation may have occurred in both low and high vaginal regions, predominantly observed by the increased concentration of pro-inflammatory cytokine TNF-alpha in patients from the placebo group (p < 0.05), while the administration of probiotics has shortened the period of inflammation as observed from the reduced need for anti-inflammatory cytokine IL-4 and IL-10 over time (p < 0.05). Taken together, our present new data further support previous findings that probiotic SynForU-HerCare had a beneficial effect against vaginal candidiasis in pregnant women via modulation of the vaginal microbiota and microenvironment.
Isolation and Characterization of Endophytes Bacterial Strains of Momordica charantia L. and Their Possible Approach in Stress Management
Authors: Singh Ritu , Pandey Kapil Deo , Singh Monika , Singh Sandeep Kumar , Hashem Abeer , Al-Arjani Al-Bandari Fahad , Abd_Allah Elsayed Fathi , Singh Prashant Kumar , Kumar Ajay
Published: January 2022

In the present study, eight endophytic bacterial strains, namely Bacillus licheniformis R1, Bacillus sp. R2, Agrobacterium tumefaciens R6, uncultured bacterium R11, Bacillus subtilis RS3, Bacillus subtilis RS6, uncultured bacterium RS8 and Lysinibacillus fusiformis RS9, were isolated from the root of Momordica charantia L. All the strains, except R6 exhibited positive for IAA production, siderophore production, and phosphate solubilization during plant growth-promoting traits analysis. Strains invariably utilized glucose and sucrose as a carbon source during substrate utilization, while yeast extract, ammonium sulphate, ammonium chloride, glycine, glutamine, and isoleucine as nitrogen sources. In addition, Spectinomycin was found as the most effective during antibiotic sensitivity TEST, followed by Chloramphenicol, Erythromycin, Rifampicin and Kanamycin, while Polymixin B was found least effective, while strains R1, R6, and RS8 were sensitive to all the antibiotics. Strains R1 and RS6 were able to withstand tolerance up to 10% of NaCl. The strains showing resistance against broad-spectrum antibiotics, especially chloramphenicol, can be used in hospital waste management. In addition, strains with a tolerance of 10 % of NaCl can improve plant growth in the saline affected area.
Correlation between Phenotypic and In Silico Detection of Antimicrobial Resistance in Salmonella enterica in Canada Using Staramr
Authors: Bharat Amrita , Petkau Aaron , Avery Brent P. , Chen Jessica C. , Folster Jason P. , Carson Carolee A. , Kearney Ashley , Nadon Celine , Mabon Philip , Thiessen Jeffrey , Alexander David C. , Allen Vanessa , El Bailey Sameh , Bekal Sadjia , German Greg J. , Haldane David , Hoang Linda , Chui Linda , Minion Jessica , Zahariadis George , Domselaar Gary Van , Reid-Smith Richard J. , Mulvey Michael R.
Published: January 2022

Whole genome sequencing (WGS) of Salmonella supports both molecular typing and detection of antimicrobial resistance (AMR). Here, we evaluated the correlation between phenotypic antimicrobial susceptibility testing (AST) and in silico prediction of AMR from WGS in Salmonella enterica (n = 1321) isolated from human infections in Canada. Phenotypic AMR results from broth microdilution testing were used as the gold standard. To facilitate high-throughput prediction of AMR from genome assemblies, we created a tool called Staramr, which incorporates the ResFinder and PointFinder databases and a custom gene-drug key for antibiogram prediction. Overall, there was 99% concordance between phenotypic and genotypic detection of categorical resistance for 14 antimicrobials in 1321 isolates (18,305 of 18,494 results in agreement). We observed an average sensitivity of 91.2% (range 80.5–100%), a specificity of 99.7% (98.6–100%), a positive predictive value of 95.4% (68.2–100%), and a negative predictive value of 99.1% (95.6–100%). The positive predictive value of gentamicin was 68%, due to seven isolates that carried aac(3)-IVa, which conferred MICs just below the breakpoint of resistance. Genetic mechanisms of resistance in these 1321 isolates included 64 unique acquired alleles and mutations in three chromosomal genes. In general, in silico prediction of AMR in Salmonella was reliable compared to the gold standard of broth microdilution. WGS can provide higher-resolution data on the epidemiology of resistance mechanisms and the emergence of new resistance alleles.
A One-Stop Protocol to Assess Myocardial Fibrosis in Frozen and Paraffin Sections
Authors: Sridharan Divya , Pracha Nooruddin , Dougherty Julie A. , Akhtar Ali , Alvi Syed Baseeruddin , Khan Mahmood
Published: January 2022

Masson’s Trichrome Staining (MTS) is a useful tool for analyzing fibrosis in a plethora of disease pathologies by differential staining of tissue components. It is used to identify collagen fibers in different tissues like heart, lung, skin, and muscles. Especially in cardiac fibrosis, MTS stains the collagen fibers (blue color), which helps in the distinction of scar area versus the healthy area (red color). However, there are several challenges to stain both paraffin-embedded sections and frozen (cryosections) using a single protocol. Therefore, the goal of this study was to develop a simple short protocol to assess cardiac fibrosis in both paraffin-embedded and cryo heart sections. MTS uses three different stains, i.e., Weigert’s Iron Hematoxylin, Biebrich scarlet-acid fuchsin, and aniline blue to detect nuclei, cytoplasm, and collagen, respectively. In this study, we developed a simple short protocol that can be adapted by any lab to easily assess cardiac fibrosis in paraffin and frozen heart sections. Furthermore, we have addressed the challenges that are commonly faced during the immunostaining process and troubleshooting techniques. Overall, we have successfully developed a simple one-step protocol to assess myocardial fibrosis in paraffin-embedded and frozen cryosections.
A Combined Supplement of Probiotic Strains AP-32, bv-77, and CP-9 Increased Akkermansia mucinphila and Reduced Non-Esterified Fatty Acids and Energy Metabolism in HFD-Induced Obese Rats
Authors: Liao Chorng-An , Huang Cheng-Hsieh , Ho Hsieh-Hsun , Chen Jui-Fen , Kuo Yi-Wei , Lin Jia-Hung , Tsai Shin-Yu , Tsai Hui-Yun , Yeh Yao-Tsung
Published: January 2022

Obesity is referred to as a condition in which excess body fat has accumulated to an extent that it causes negative impacts on health. The formation of body fat is regulated by complicated networks in relation to energy metabolism, and gut microbiota have been regarded as a key player. Studies have shown that supplements of probiotics provide benefits to health, including an improvement in metabolic syndrome and the control of body weight. In the present study, three probiotic strains, AP-32, bv-77, and CP-9, stood out from nine candidates using a lipid consumption assay, and were subsequently introduced to further animal tests. A rodent model of obesity was induced by a high-fat diet (HFD) in Sprague-Dawley (SD) rats, and three probiotic strains were administered either separately or in a mixture. A low dose (5 × 109 CFU/kg/day) and a high dose (2.5 × 1010 CFU/kg/day) of probiotics were orally provided to obese rats. The bioeffects of the probiotic supplements were evaluated based on five aspects: (1) the body weight and growth rate; (2) ketone bodies, non-esterified fatty acids (NEFAs), and feed efficiency; (3) blood biochemistry; (4) fat content; and (5) gut microbiota composition. Our results demonstrated that the supplement of AP-32, CP-9, and bv-77 alleviated the increasing rate of body weight and prevented the elevation of NEFAs and ketone bodies in obese rats. Although the effect on fat content showed a minor improvement, the supplement of probiotics displayed significant improvements in HFD-induced poor blood biochemical characteristics, such as alanine aminotransferase (ALT), aspartate Transaminase (AST), and uric acid, within 4 weeks. Furthermore, the combined supplement of three strains significantly increased Akkermansia mucinphila as compared with three individual strains, while its enrichment was negatively correlated with NEFAs and energy metabolism. In general, a mixture of three probiotic strains delivered a better outcome than a single strain, and the high dose of supplements provided a more profound benefit than the low dose. In conclusion, three probiotic strains, AP-32, bv-77, and CP-9, can alleviate body fat formation in obese rats. Furthermore, a combined supplement of these three probiotic strains may have potential in treating or controlling metabolic disorders.
Validating Accuracy of a Mobile Application against Food Frequency Questionnaire on Key Nutrients with Modern Diets for mHealth Era
Authors: Kusuma Joyce D. , Yang Hsiao-Ling , Yang Ya-Ling , Chen Zhao-Feng , Shiao Shyang-Yun Pamela Koong
Published: January 2022

In preparation for personalized nutrition, an accurate assessment of dietary intakes on key essential nutrients using smartphones can help promote health and reduce health risks across vulnerable populations. We, therefore, validated the accuracy of a mobile application (app) against Food Frequency Questionnaire (FFQ) using artificial intelligence (AI) machine-learning-based analytics, assessing key macro- and micro-nutrients across various modern diets. We first used Bland and Altman analysis to identify and visualize the differences between the two measures. We then applied AI-based analytics to enhance prediction accuracy, including generalized regression to identify factors that contributed to the differences between the two measures. The mobile app underestimated most macro- and micro-nutrients compared to FFQ (ranges: −5% for total calories, −19% for cobalamin, −33% for vitamin E). The average correlations between the two measures were 0.87 for macro-nutrients and 0.84 for micro-nutrients. Factors that contributed to the differences between the two measures using total calories as an example, included caloric range (1000–2000 versus others), carbohydrate, and protein; for cobalamin, included caloric range, protein, and Chinese diet. Future studies are needed to validate actual intakes and reporting of various diets, and to examine the accuracy of mobile App. Thus, a mobile app can be used to support personalized nutrition in the mHealth era, considering adjustments with sources that could contribute to the inaccurate estimates of nutrients.
Sex-Heterogeneity on the Association between Dietary Patterns at 4 Years of Age with Adiposity and Cardiometabolic Risk Factors at 10 Years of Age
Authors: Durão Catarina , Severo Milton , Oliveira Andreia , Lopes Carla
Published: January 2022

To evaluate the association of dietary patterns (DP) at 4 years with adiposity and cardiometabolic risk factors at 10 years, considering sex-heterogeneity. This prospective analysis included 3823 children enrolled in the population-based birth cohort, Generation XXI (Porto-Portugal, 2005–2006). Diet at 4 years was assessed by FFQ, with three DP being identified: high in energy-dense foods (EDF), intermediate in snacks (snacking), and healthier (reference). BMI at 10 years was considered as the Z-score according to the WHO. Other adiposity indicators—fat mass percentage (FM%), fat mass index (FMI), and waist-to-height ratio (WHtR)—were converted to z-scores using the sample’s sex-specific means and standard deviations, as were the cardiometabolic risk factors (systolic and diastolic blood pressure, lipid profile, and insulin resistance). The associations of DP at 4 years with later adiposity or cardiometabolic factors were estimated by linear regression or by multinomial logistic regression models. In fully adjusted models, the EDF DP was significantly positively associated with the BMI (EDF vs. healthier: β = 0.139; 95% CI: 0.031, 0.246, P-interaction = 0.042) and obesity (OR = 2.68; 95% CI 1.55, 4.63, P-interaction = 0.005) only in girls, among whom, it increased insulin (β = 0.165; 95% CI: 0.020, 0.311) and HOMA-IR (β = 0.159; 95% CI: 0.013, 0.306) at 10 years. An EDF DP at 4 years is associated with later adiposity, insulin, and HOMA-IR in girls.
Methods for the Determination of Transition Metal Impurities in Cyclotron-Produced Radiometals
Authors: Forgács Viktória , Fekete Anikó , Gyuricza Barbara , Szücs Dániel , Trencsényi György , Szikra Dezső
Published: January 2022

Cyclotron-produced radiometals must be separated from the irradiated target and purified from other metal impurities, which could interfere with the radiolabeling process. We compared different chromatographic and colorimetric methods to determine the amount of transition metals in radioactive samples. Besides commercially available colorimetric tests, 4-(2-pyridylazo)resorcinol and xylenol orange were used as a non-selective metal reagents, forming water-soluble chelates with most of the transition metals immediately. We compared the applicability of pre- and post-column derivatization, as well as colorimetric determination without separation. The studied chromatographic and colorimetric analyses are not suitable to completely replace atomic spectroscopic techniques for the determination of metal contaminants in radioactive samples, but they may play an important role in the development of methods for the purification of radiometals and in their routine quality control.
From Ama Lur to the Anthropocene and Back: The Earth in Basque Mythology
Authors: Garagalza Luis
Published: January 2022

What I propose is that, by delving into the world of mythologies, there we might find some indications, helpful for understanding what is happening with the environment today. To do so, I will revisit a particular mythology from the South of Europe, an archaic (probably, a pre-Indo-European one), namely Basque mythology. Here, earth (lurra) appears as a maternal character (Ama-lur) and becomes, in a sense, divine in the figure of the goddess Mari, who occupied a central and predominant position in this cosmovision.
UV Photolysis Study of Para-Aminobenzoic Acid Using Parahydrogen Matrix Isolated Spectroscopy
Authors: McKinnon Alexandra , Moore Brendan , Djuricanin Pavle , Momose Takamasa
Published: January 2022

Many sunscreen chemical agents are designed to absorb UVB radiation (and in some cases UVA) to protect the skin from sunlight, but UV absorption is often accompanied by photodissociation of the chemical agent, which may reduce its UV absorption capacity. Therefore, it is important to understand the photochemical processes of sunscreen agents. In this study, the photolysis of para-aminobenzoic acid (PABA), one of the original sunscreen chemical agents, at three different UV ranges (UVA: 355 nm, UVB: >280 nm, and UVC: 266 nm and 213 nm) was investigated using parahydrogen (pH2) matrix isolation Fourier-Transform Infrared (FTIR) Spectroscopy. PABA was found to be stable under UVA (355 nm) irradiation, while it dissociated into 4-aminylbenzoic acid (the PABA radical) through the loss of an amino hydrogen atom under UVB (>280 nm) and UVC (266 nm and 213 nm) irradiation. The radical production supports a proposed mechanism of carcinogenic PABA-thymine adduct formation. The infrared spectrum of the PABA radical was analyzed by referring to quantum chemical calculations, and two conformers were found in solid pH2. The PABA radicals were stable in solid pH2 for hours after irradiation. The trans-hydrocarboxyl (HOCO) radical was also observed as a minor secondary photoproduct of PABA following 213 nm irradiation. This work shows that pH2 matrix isolation spectroscopy is effective for photochemical studies of sunscreen agents.
Phytochemical Composition and Antioxidant Activity of Passiflora spp. Germplasm Grown in Ecuador
Authors: Viera William , Shinohara Takashi , Samaniego Iván , Sanada Atsushi , Terada Naoki , Ron Lenin , Suárez-Tapia Alfonso , Koshio Kaihei
Published: January 2022

Tropical fruits are in high demand for their flavor and for their functional composition because these compounds are considered nutraceuticals. Passion fruit production is of economic importance to Ecuador; however, several Passiflora species are grown and each has to be analyzed to identify their phytochemical composition. In this study, the polyphenol, flavonoid, carotenoid, vitamin C, sugar and organic acid contents were determined. Six different Passiflora spp. germplasms were analyzed, coming from Passiflora edulis f. flavicarpa, Passiflora alata, Passiflora edulis f. edulis and unidentified Passiflora species (local germplasm). Measurement techniques included reflectometry for vitamin C, spectrophotometry for antioxidant compounds and HPLC for sugars and organic acids. Data were analyzed by principal component analysis, correlation and analysis of variance. Results showed that INIAP 2009 and P10 showed a high amount of polyphenols, antioxidant activity and citric content. Sweet passion fruit had the lowest vitamin C content while Gulupa showed the highest content. In terms of the local germplasm, POR1 showed the lowest content of flavonoids while PICH1 had high flavonoid and carotenoid content. Polyphenols were the main compounds that influenced antioxidant activity. This phytochemical information adds value to passion fruit as a nutraceutical source.