bluuebunny/crossref_metadata_2025 huggingface dataset:
Retrospective Validation of a 168-Gene Expression Signature for Glioma Classification on a Single Molecule Counting Platform
Authors: Tran Paul Minh Huy , Tran Lynn Kim Hoang , Satter Khaled bin , Purohit Sharad , Nechtman John , Hopkins Diane I. , dos Santos Bruno , Bollag Roni , Kolhe Ravindra , Sharma Suash , She Jin Xiong
Published: January 2021

Gene expression profiling has been shown to be comparable to other molecular methods for glioma classification. We sought to validate a gene-expression based glioma classification method. Formalin-fixed paraffin embedded tissue and flash frozen tissue collected at the Augusta University (AU) Pathology Department between 2000–2019 were identified and 2 mm cores were taken. The RNA was extracted from these cores after deparaffinization and bead homogenization. One hundred sixty-eight genes were evaluated in the RNA samples on the nCounter instrument. Forty-eight gliomas were classified using a supervised learning algorithm trained by using data from The Cancer Genome Atlas. An ensemble of 1000 linear support vector models classified 30 glioma samples into TP1 with classification confidence of 0.99. Glioma patients in TP1 group have a poorer survival (HR (95% CI) = 4.5 (1.3–15.4), p = 0.005) with median survival time of 12.1 months, compared to non-TP1 groups. Network analysis revealed that cell cycle genes play an important role in distinguishing TP1 from non-TP1 cases and that these genes may play an important role in glioma survival. This could be a good clinical pipeline for molecular classification of gliomas.
Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2+ γδ T Cell Alterations with Lymph Node Invasion
Authors: Fattori Stéphane , Gorvel Laurent , Granjeaud Samuel , Rochigneux Philippe , Rouvière Marie-Sarah , Ben Amara Amira , Boucherit Nicolas , Paul Magali , Dauplat Marie Mélanie , Thomassin-Piana Jeanne , Paciencia-Gros Maria , Avenin Morgan , Pakradouni Jihane , Barrou Julien , Charafe-Jauffret Emmanuelle , Houvenaeghel Gilles , Lambaudie Eric , Bertucci François , Goncalves Anthony , Tarpin Carole , Nunès Jacques A. , Devillier Raynier , Chretien Anne-Sophie , Olive Daniel
Published: January 2021

The rationale for therapeutic targeting of Vδ2+ γδ T cells in breast cancer is strongly supported by in vitro and murine preclinical investigations, characterizing them as potent breast tumor cell killers and source of Th1-related cytokines, backing cytotoxic αβ T cells. Nonetheless, insights regarding Vδ2+ γδ T cell phenotypic alterations in human breast cancers are still lacking. This paucity of information is partly due to the challenging scarcity of these cells in surgical specimens. αβ T cell phenotypic alterations occurring in the tumor bed are detectable in the periphery and correlate with adverse clinical outcomes. Thus, we sought to determine through an exploratory study whether Vδ2+ γδ T cells phenotypic changes can be detected within breast cancer patients’ peripheral blood, along with association with tumor progression. By using mass cytometry, we quantified 130 immune variables from untreated breast cancer patients’ peripheral blood. Supervised analyses and dimensionality reduction algorithms evidenced circulating Vδ2+ γδ T cell phenotypic alterations already established at diagnosis. Foremost, terminally differentiated Vδ2+ γδ T cells displaying phenotypes of exhausted senescent T cells associated with lymph node involvement. Thereby, our results support Vδ2+ γδ T cells implication in breast cancer pathogenesis and progression, besides shedding light on liquid biopsies to monitor surrogate markers of tumor-infiltrating Vδ2+ γδ T cell antitumor activity.
Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis
Authors: Nakamura Kodai , Hiyake Naomi , Hamada Tomofumi , Yokoyama Seiya , Mori Kazuki , Yamashiro Kouta , Beppu Mahiro , Sagara Yasuaki , Sagara Yoshiaki , Sugiura Tsuyoshi
Published: January 2021

A lack of reliable biomarkers for oral squamous cell carcinoma (OSCC) poses a major clinical issue. The sensitivity and specificity of classical serum tumor markers, such as the squamous cell carcinoma antigen (SCC-Ag), are quite poor, especially for early detection. This study aimed to identify specific serum miRNAs potentially serving as OSCC biomarkers. The expression levels of candidate miRNAs in serum samples from 40 OSCC patients and 40 healthy controls were quantitatively analyzed via microarray and reverse transcription PCR (RT-PCR) analyses. To enhance the accuracy of detection, we used Fisher’s linear discriminant analysis to establish a diagnostic model that incorporated a combination of selected miRNAs. Consequently, miR-19a and miR-20a were significantly upregulated in the patient group (p = 0.014 and 0.036, respectively), whereas miR-5100 was downregulated (p = 0.001). We found that a combination of six miRNAs (miR-24, miR-20a, miR-122, miR-150, miR-4419a, and miR-5100) could distinguish between OSCC and the control group with a higher degree of accuracy (Area Under the Curve, AUC: 0.844, sensitivity: 55%, and specificity: 92.5%). Furthermore, compared to serum SCC antigen, the 6-miRNA panel could accurately detect the presence of OSCC. The present specific miRNAs panel may serve as a novel candidate biomarker of oral cancer.
Plasma-Conditioned Liquids as Anticancer Therapies In Vivo: Current State and Future Directions
Authors: Solé-Martí Xavi , Espona-Noguera Albert , Ginebra Maria-Pau , Canal Cristina
Published: January 2021

Plasma-conditioned liquids (PCL) are gaining increasing attention in the medical field, especially in oncology, and translation to the clinics is advancing on a good path. This emerging technology involving cold plasmas has great potential as a therapeutic approach in cancer diseases, as PCL have been shown to selectively kill cancer cells by triggering apoptotic mechanisms without damaging healthy cells. In this context, PCL can be injected near the tumor or intratumorally, thereby allowing the treatment of malignant tumors located in internal organs that are not accessible for direct cold atmospheric plasma (CAP) treatment. Therefore, PCL constitutes a very interesting and minimally invasive alternative to direct CAP treatment in cancer therapy, avoiding surgeries and allowing multiple local administrations. As the field advances, it is progressively moving to the evaluation of the therapeutic effects of PCL in in vivo scenarios. Exciting developments are pushing forward the clinical translation of this novel therapy. However, there is still room for research, as the quantification and identification of reactive oxygen and nitrogen species (RONS) in in vivo conditions is not yet clarified, dosage regimens are highly variable among studies, and other more relevant in vivo models could be used. In this context, this work aims to present a critical review of the state of the field of PCL as anticancer agents applied in in vivo studies.
Radiomics-Derived Data by Contrast Enhanced Magnetic Resonance in RAS Mutations Detection in Colorectal Liver Metastases
Authors: Granata Vincenza , Fusco Roberta , Avallone Antonio , De Stefano Alfonso , Ottaiano Alessandro , Sbordone Carolina , Brunese Luca , Izzo Francesco , Petrillo Antonella
Published: January 2021

Purpose: To assess the association of RAS mutation status and radiomics-derived data by Contrast Enhanced-Magnetic Resonance Imaging (CE-MRI) in liver metastases. Materials and Methods: 76 patients (36 women and 40 men; 59 years of mean age and 36–80 years as range) were included in this retrospective study. Texture metrics and parameters based on lesion morphology were calculated. Per-patient univariate and multivariate analysis were made. Wilcoxon-Mann-Whitney U test, receiver operating characteristic (ROC) analysis, pattern recognition approaches with features selection approaches were considered. Results: Significant results were obtained for texture features while morphological parameters had not significant results to classify RAS mutation. The results showed that using a univariate analysis was not possible to discriminate accurately the RAS mutation status. Instead, considering a multivariate analysis and classification approaches, a KNN exclusively with texture parameters as predictors reached the best results (AUC of 0.84 and an accuracy of 76.9% with 90.0% of sensitivity and 67.8% of specificity on training set and an accuracy of 87.5% with 91.7% of sensitivity and 83.3% of specificity on external validation cohort). Conclusions: Texture parameters derived by CE-MRI and combined using multivariate analysis and patter recognition approaches could allow stratifying the patients according to RAS mutation status.
Catalytic Reaction of Carbon Dioxide with Methane on Supported Noble Metal Catalysts
Authors: Erdőhelyi András
Published: January 2021

The conversion of CO2 and CH4, the main components of the greenhouse gases, into synthesis gas are in the focus of academic and industrial research. In this review, the activity and stability of different supported noble metal catalysts were compared in the CO2 + CH4 reaction on. It was found that the efficiency of the catalysts depends not only on the metal and on the support but on the particle size, the metal support interface, the carbon deposition and the reactivity of carbon also influences the activity and stability of the catalysts. The possibility of the activation and dissociation of CO2 and CH4 on clean and on supported noble metals were discussed separately. CO2 could dissociate on metal surfaces, this reaction could proceed via the formation of carbonate on the support, or on the metal–support interface but in the reaction the hydrogen assisted dissociation of CO2 was also suggested. The decrease in the activity of the catalysts was generally attributed to carbon deposition, which can be formed from CH4 while others suggest that the source of the surface carbon is CO2. Carbon can occur in different forms on the surface, which can be transformed into each other depending on the temperature and the time elapsed since their formation. Basically, two reaction mechanisms was proposed, according to the mono-functional mechanism the activation of both CO2 and CH4 occurs on the metal sites, but in the bi-functional mechanism the CO2 is activated on the support or on the metal–support interface and the CH4 on the metal.
Parkinson’s Disease: Potential Actions of Lithium by Targeting the WNT/β-Catenin Pathway, Oxidative Stress, Inflammation and Glutamatergic Pathway
Authors: Vallée Alexandre , Vallée Jean-Noël , Lecarpentier Yves
Published: January 2021

Parkinson’s disease (PD) is one of the major neurodegenerative diseases (ND) which presents a progressive neurodegeneration characterized by loss of dopamine in the substantia nigra pars compacta. It is well known that oxidative stress, inflammation and glutamatergic pathway play key roles in the development of PD. However, therapies remain uncertain and research for new treatment is mandatory. This review focuses on the potential effects of lithium, as a potential therapeutic strategy, on PD and some of the presumed mechanisms by which lithium provides its benefit properties. Lithium medication downregulates GSK-3beta, the main inhibitor of the WNT/β-catenin pathway. The stimulation of the WNT/β-catenin could be associated with the control of oxidative stress, inflammation, and glutamatergic pathway. Future prospective clinical trials could focus on lithium and its different and multiple interactions in PD.
Acknowledgment to Reviewers of Climate in 2020
Authors:   Climate Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Climate maintains its standards for the high quality of its published papers [...]
Upconversion Nanoparticles Encapsulated with Amorphous Silica and Their Emission Quenching by FRET: A Nanosensor Excited by NIR for Mercury Detection
Authors: Wu Wei , Wei Wei , Xu Dingli , Liu Yunpeng , Li Jin , Gan Kaifeng , Liu Liang
Published: January 2021

Near-infrared (NIR) region has been considered as a diagnostic window since it avoids sample autofluorescence and light scattering. Upconversion nanoparticles (UCNPs) convert NIR light into high energy excitation light, making them a suitable excitation source for nanoprobes with deep penetration depth and high signal-to-noise ratio. The current work reported a rhodamine-derived probe for the detection of Hg(II). Corresponding absorption and emission responses for Hg(II) and detailed recognizing mechanism were discussed. An absorption titration experiment was performed. It was found that Hg(II) directly bonded with probe with chemical stoichiometry of 1:1, its association constant was calculated as 2.59 × 105 M−1. Such a high value indicated a direct coordination affinity between Hg(II) and this rhodamine-derived probe. Most metal cations exerted no increasing effect on the probe emission or absorption, exhibiting good sensing selectivity of probe towards Hg(II). Upconversion nanoparticles (UCNPs) were firstly encapsulated with silica (SiO2) and then bonded with the probe via a covalent bond. Given a near-infrared (NIR) laser excitation with wavelength of 980 nm, this probe, (E)-2-((3′,6′-bis(diethylamino)-2′,7′-dimethyl-3-oxospiro[isoindoline-1,9′-xanthen]-2-yl)imino)acetaldehyde (denoted as RHO), captured the energy of UCNPs via a FRET (fluorescence resonance energy transfer) path, resulting in the emission quenching of UCNPs. This composite system showed linear sensing behavior towards Hg(II) with high selectivity, which was similar to the case of pure probe. No probe emission, however, was observed from the composite system, which was different from the case of most literature reports. The self-quenching between probe molecules was claimed responsible for the probe emission, which was confirmed by experiment result and analysis. To the best of our knowledge, this is the first demonstration of covalently integrating SiO2-coated UCNPs with a rhodamine-derived probe for Hg(II) sensing.
Acknowledgment to Reviewers of Crystals in 2020
Authors:   Crystals Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Crystals maintains its standards for the high quality of its published papers [...]
Donor–Recipient Story in Allogeneic Hematopoietic Stem Cell Transplantation
Authors: Kum Elena , Jagelaviciute Gabriele , Li Edward , Williams Kenneth , Thyagu Santhosh , Fingrut Warren
Published: January 2021

Patients with a variety of blood, immune, and metabolic disorders may require an allogeneic hematopoietic stem cell transplant as part of their treatment. However, over 70% of these patients do not have a matched sibling donor and require an alternative donor, such as a matched unrelated donor. We present a multi-part story of a Canadian stem cell recipient who underwent transplantation for treatment of refractory chronic myelogenous leukemia, and the matched unrelated donor who saved his life. The story segments feature excerpts from interviews with the donor and the recipient, along with representative images of both storytellers. The excerpts were optimized for publication on social media and were arranged to build a story arc that parallels the journey of the donor and recipient together. This donor-recipient story may serve as a resource to help raise awareness about stem cell donation and to encourage eligible individuals to register as donors. The story is one of several developed by Why We Swab, a library of stories in stem cell donation in Canada (Facebook, Twitter, and Instagram; @WhyWeSwab) to support the recruitment of committed unrelated donors.
Prophylactic Surgery in the BRCA+ Patient: Do Women Develop Breast Cancer While Waiting?
Authors: Macadam Sheina A. , Slater Karen , Cheifetz Rona E. , Jansen Leigh , Chia Stephen , Brasher Penelope M. A. , Bovill Esta S.
Published: January 2021

Breast cancer susceptibility gene (BRCA) mutation carriers have an increased risk of breast cancer. Mitigation of this risk can be achieved via surveillance or prophylactic mastectomy with or without breast reconstruction. Those that choose surgery expect to reduce their chance of developing cancer. The purpose of this study was to determine the incidence of patients developing breast cancer prior to surgery and to identify modifiable contributing factors within the patient journey. This is a historical cohort study of all BRCA mutation carriers identified through the British Columbia Cancer Hereditary Cancer Program between 2000 and 2012. Patients were divided into two groups: surveillance (S) and prophylactic mastectomy with immediate breast reconstruction (PM/IBR). The incidence of cancer, time to PM/IBR and patient journeys were analyzed. A total of 333 women were identified. The time to surgery from mutation disclosure was a median of 31 (5.3, 75.7) months. During this period, 6% of patients developed breast cancer compared with a 14% incidence of breast cancer in patients choosing surveillance. The majority of time to surgery was attributed to the period between mutation disclosure and the decision to proceed with surgery. Strategies to facilitate decision-making as well as wait list prioritization and dedicated operative time should be targeted to this population to decrease the number of women developing an interval cancer prior to surgery.
Subterranean Waters of Yucatán Peninsula, Mexico Reveal Epigean Species Dominance and Intraspecific Variability in Freshwater Ostracodes (Crustacea: Ostracoda)
Authors: Macario-González Laura , Cohuo Sergio , Angyal Dorottya , Pérez Liseth , Mascaró Maite
Published: January 2021

The Yucatán Peninsula is a karstic region, rich in subterranean environments with a diverse crustacean stygobiont fauna. In order to gain insights into the biological evolution of the subterranean environments of this region, we evaluated the ostracode species composition of caves and cenotes in five independent sampling campaigns (2008, 2013, 2017–2019). Using morphometric analyses, we evaluated inter-population morphological variability; using molecular analysis based on mitochondrial COI and nuclear 18S rDNA, we evaluated genetic differentiation in selected species. The observed fauna is composed of 20 (epigean) species, presenting a lack of strict stygobionts. Morphometric analyses discriminated up to three morphotypes in each of the three most abundant species: Cytheridella ilosvayi, Alicenula sp. and Cypridopsis vidua. High intraspecific morphological variability was found either in shape or size. Phylogenetic analysis based on COI demonstrated the existence of three lineages on C. ilosvayi, with high support (>0.9). The 18S rDNA sequences were identical among individuals of different populations. A lack of congruence between the genetic markers precluded us from postulating speciation in subterranean environments. It is likely that Late Pleistocene—Early Holocene climate variation related to sea level and precipitation was forcing agent for epigean ostracode dominance in subterranean environments of the Peninsula.
Systematics and Conservation of Neotropical Amphibians and Reptiles
Authors: Catenazzi Alessandro , von May Rudolf
Published: January 2021

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Acknowledgment to Reviewers of Designs in 2020
Authors:   Designs Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Designs maintains its standards for the high quality of its published papers [...]
Acknowledgment to Reviewers of Education Sciences in 2020
Authors:   Education Sciences Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Education Sciences maintains its standards for the high quality of its published papers [...]
Empowering Forest Owners with Simple Volume Equations for Poplar Plantations in the Órbigo River Basin (NW Spain)
Authors: Blanco Roberto , Blanco Juan A.
Published: January 2021

Hybrid poplar plantations are becoming increasingly important as a source of income for farmers in northwestern Spain, as rural depopulation and farmers aging prevent landowners from planting other labor-intensive crops. However, plantation owners, usually elderly and without formal forestry background, lack of simple tools to estimate the size and volume of their plantations by themselves. Therefore, farmers are usually forced to rely on the estimates made by the timber companies that are buying their trees. With the objective of providing a simple, but empowering, tool for these forest owners, simple equations based only on diameter were developed to estimate individual tree volume for the Órbigo River basin. To do so, height and diameter growth were measured for 10 years (2009–2019) in 404 trees growing in three poplar plantations in Leon province. An average growth per tree of 1.66 cm year−1 in diameter, 1.52 m year−1 in height, and 0.03 m3 year−1 in volume was estimated, which translated into annual volume increment of 13.02 m3 ha−1 year−1. However, annual volume increment was different among plots due to their fertility, with two plots reaching maximum volume growth around 11 years since planting and another at 13 years, encompassing the typical productivity range in plantations in this region. Such data allowed developing simple but representative linear, polynomial and power equations to estimate volume explaining 93%–98% of the observed variability. Such equations can be easily implemented in any cellphone with a calculator, allowing forest owners to accurately estimate their timber existences by using only a regular measuring tape to measure tree diameter. However, models for height were less successful, explaining only 75%–76% of observed variance. Our approach to generate simplified volume equations has shown to be viable for poplar, but it could be applied to any species for which several volume equations are available.
Individual Tree Crown Segmentation Directly from UAV-Borne LiDAR Data Using the PointNet of Deep Learning
Authors: Chen Xinxin , Jiang Kang , Zhu Yushi , Wang Xiangjun , Yun Ting
Published: January 2021

Accurate individual tree crown (ITC) segmentation from scanned point clouds is a fundamental task in forest biomass monitoring and forest ecology management. Light detection and ranging (LiDAR) as a mainstream tool for forest survey is advancing the pattern of forest data acquisition. In this study, we performed a novel deep learning framework directly processing the forest point clouds belonging to the four forest types (i.e., the nursery base, the monastery garden, the mixed forest, and the defoliated forest) to realize the ITC segmentation. The specific steps of our approach were as follows: first, a voxelization strategy was conducted to subdivide the collected point clouds with various tree species from various forest types into many voxels. These voxels containing point clouds were taken as training samples for the PointNet deep learning framework to identify the tree crowns at the voxel scale. Second, based on the initial segmentation results, we used the height-related gradient information to accurately depict the boundaries of each tree crown. Meanwhile, the retrieved tree crown breadths of individual trees were compared with field measurements to verify the effectiveness of our approach. Among the four forest types, our results revealed the best performance for the nursery base (tree crown detection rate r = 0.90; crown breadth estimation R2 > 0.94 and root mean squared error (RMSE) < 0.2m). A sound performance was also achieved for the monastery garden and mixed forest, which had complex forest structures, complicated intersections of branches and different building types, with r = 0.85, R2 > 0.88 and RMSE < 0.6 m for the monastery garden and r = 0.80, R2 > 0.85 and RMSE < 0.8 m for the mixed forest. For the fourth forest plot type with the distribution of crown defoliation across the woodland, we achieved the performance with r = 0.82, R2 > 0.79 and RMSE < 0.7 m. Our method presents a robust framework inspired by the deep learning technology and computer graphics theory that solves the ITC segmentation problem and retrieves forest parameters under various forest conditions.
Climate Change Effects on Trophic Interactions of Bark Beetles in Inner Alpine Scots Pine Forests
Authors: Wermelinger Beat , Rigling Andreas , Schneider Mathis Doris , Kenis Marc , Gossner Martin M.
Published: January 2021

Increased tree mortality has become a widespread phenomenon and is largely attributed to climate change. Little field research has addressed the complex interactions between trees, herbivores, and their natural enemies as affected by temperature. We recorded the densities of bark insects and their natural enemies emerging from felled trees in Scots pine forests at 17 study sites along 6 elevation gradients encompassing different temperature ranges in 3 regions in Switzerland and Italy. We additionally measured tree resin defense at different elevations. The density of aggressive bark beetles decreased with increasing temperatures while that of non-aggressive species did not respond to temperature. Contrasting patterns were also found for natural enemies, with the densities of most predatory taxa decreasing with increasing temperature whereas densities of parasitoids increased. Consequently, bark beetle mortality by predators decreased and that by parasitoids increased with temperature. Exudation of resin increased with temperature. As the number of resin ducts did not change with temperature, this is assumed a physical effect of reduced viscosity. Despite lower densities of aggressive bark beetles and improved tree resin flow under higher temperatures, the currently experienced drought-induced reduction in tree vigor is likely to increase tree mortality under the ongoing climate warming.
Filaments, Fronts and Eddies in the Cabo Frio Coastal Upwelling System, Brazil
Authors: Calil Paulo H. R. , Suzuki Nobuhiro , Baschek Burkard , da Silveira Ilson C. A.
Published: January 2021

We investigate the dynamics of meso- and submesoscale features of the northern South Brazil Bight shelf region with a 500-m horizontal resolution regional model. We focus on the Cabo Frio upwelling center, where nutrient-rich, coastal waters are transported into the mid- and outer shelf, because of its importance for local and remote productivity. The Cabo Frio upwelling center undergoes an upwelling phase, from late September to March, and a relaxation phase, from April to early September. During the upwelling phase, an intense front around 200 km long and 20 km wide with horizontal temperature gradients as large as 8 ∘C over less than 10 km develops. A surface-intensified frontal jet of 0.7 ms−1 in the upper 20 m and velocities of around 0.3 ms−1 reaching down to 65 m depth makes this front a preferential cross-shelf transport pathway. Large vertical mixing and vertical velocities are observed within the frontal region. The front is associated with strong cyclonic vorticity and strong variance in relative vorticity, frequently with O(1) Rossby numbers. The dynamical balance within the front is between the pressure gradient, Coriolis and vertical mixing terms, which are induced both by the winds, during the upwelling season, and by the geostrophic frontal jet. Therefore, the frontal dynamics may be largely described as sum of Ekman and turbulent thermal wind balances. During the upwelling phase, a mix of barotropic and baroclinic instabilities dominates in the upwelling center. However, these instabilities do not lead to the local formation of coherent eddies when the front is strong. In the relaxation phase, the front vanishes, and the water column becomes less stratified. The interaction between eastward coastal currents generated by sea level variability, coastal intrusions of the Brazil Current, and sporadic wind-driven, coastal upwelling events induce the formation of cyclonic eddies with diameters of, approximately, 20 km. They are in gradient-wind balance and propagate along the 100-m isobath on the shelf. During this phase baroclinic instability dominates. Cold filaments with widths of 2 km are formed due to straining and stretching of cold, coastal temperature anomalies. They last for a few days and are characterized by downwelling as large as 1 cms−1. The turbulent thermal wind balance provides a good first order estimate of the dynamical balance within the filament, but vertical and horizontal advection are shown to be important. To our knowledge, this is the first account of these smaller scale features in the region. Because these meso- and submesoscale features on the shelf heavily affect the water properties crucial to productivity of the South Brazil Bight, it is important to take these features into account for a better understanding of the functioning of this ecosystem and its resilience to both direct human activities as well as to climate change.
Investigating the Role of Psychological, Social, Religious and Ethical Determinants on Consumers’ Purchase Intention and Consumption of Convenience Food
Authors: Imtiyaz Hena , Soni Peeyush , Yukongdi Vimolwan
Published: January 2021

Despite impressive market growth, increasing demand and economic importance of convenience food in emerging economies such as India, comprehensive research regarding the role of psychological and social determinants on convenience food choice is lacking. Therefore, this research aims to investigate the influence of convenience orientation, social status, moral attitude, mood, spiritual concern, religious beliefs and ethical values on purchase intention and consumption of convenience food. The non-probability purposive sampling method was adopted for recruitment of participants. A pre-tested questionnaire was used to collect data from 501 consumers. The descriptive statistics, confirmatory factor analysis and structural equation modeling were carried out to analyse the data. The factor loading, Cronbach’s alpha, composite reliability, average variance extracted, and correlations demonstrated good internal consistency and reliability of scale items as well as convergent and discriminant validity of the constructs. The model fit indices revealed that measurement and structural models fitted well with data. The path analysis of the structural model demonstrated that convenience orientation (β = 0.789 ***, t = 32.462), moral attitude (β = 0.594 ***, t = 20.984), mood (β = 0.586 ***, t = 18.683), spiritual concern (β = 0.145 ***, t = 3.23), religious beliefs (β = 0.451 ***, t = 14.787) and ethical values (β = 0.497 ***, t = 16.678) were positively related with purchase intention and consumption of convenience food (*** Significant at p ≤ 0.01). The path analysis of structural model also indicated that social status was not linked with purchase intention and consumption of convenience food. The convenience orientation was the key determinant influencing purchase intention and consumption of convenience food.
Acknowledgment to Reviewers of Galaxies in 2020
Authors:   Galaxies Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Galaxies maintains its standards for the high quality of its published papers [...]
Topical Mucoadhesive Alginate-Based Hydrogel Loading Ketorolac for Pain Management after Pharmacotherapy, Ablation, or Surgical Removal in Condyloma Acuminata
Authors: El Moussaoui Salima , Fernández-Campos Francisco , Alonso Cristina , Limón David , Halbaut Lyda , Garduño-Ramirez Maria Luisa , Calpena Ana Cristina , Mallandrich Mireia
Published: January 2021

Condyloma acuminata is an infectious disease caused by the human papilloma virus (HPV) and one of the most common sexually transmitted infections. It is manifested as warts that frequently cause pain, pruritus, burning, and occasional bleeding. Treatment (physical, chemical, or surgical) can result in erosion, scars, or ulcers, implying inflammatory processes causing pain. In this work, a biocompatible topical hydrogel containing 2% ketorolac tromethamine was developed to manage the painful inflammatory processes occurring upon the removal of anogenital condylomas. The hydrogel was physically, mechanically, and morphologically characterized: it showed adequate characteristics for a topical formulation. Up to 73% of ketorolac in the gel can be released following a one-phase exponential model. Upon application on human skin and vaginal mucosa, ketorolac can permeate through both of these and it can be retained within both tissues, particularly on vaginal mucosa. Another advantage is that no systemic side effects should be expected after application of the gel. The hydrogel showed itself to be well tolerated in vivo when applied on humans, and it did not cause any visible irritation. Finally, ketorolac hydrogel showed 53% anti-inflammatory activity, suggesting that it is a stable and suitable formulation for the treatment of inflammatory processes, such as those occurring upon chemical or surgical removal of anogenital warts.
Functional Haplotype of LIPC Induces Triglyceride-Mediated Suppression of HDL-C Levels According to Genome-Wide Association Studies
Authors: Liao Yu-Huang , Er Leay-Kiaw , Wu Semon , Ko Yu-Lin , Teng Ming-Sheng
Published: January 2021

Hepatic lipase (encoded by LIPC) is a glycoprotein in the triacylglycerol lipase family and mainly synthesized in and secreted from the liver. Previous studies demonstrated that hepatic lipase is crucial for reverse cholesterol transport and modulating metabolism and the plasma levels of several lipoproteins. This study was conducted to investigate the suppression effect of high-density lipoprotein cholesterol (HDL-C) levels in a genome-wide association study and explore the possible mechanisms linking triglyceride (TG) to LIPC variants and HDL-C. Genome-wide association data for TG and HDL-C were available for 4657 Taiwan-biobank participants. The prevalence of haplotypes in the LIPC promoter region and their effects were calculated. The cloned constructs of the haplotypes were expressed transiently in HepG2 cells and evaluated in a luciferase reporter assay. Genome-wide association analysis revealed that HDL-C was significantly associated with variations in LIPC after adjusting for TG. Three haplotypes (H1: TCG, H2: CTA and H3: CCA) in LIPC were identified. H2: CTA was significantly associated with HDL-C levels and H1: TCG suppressed HDL-C levels when a third factor, TG, was included in mediation analysis. The luciferase reporter assay further showed that the H2: CTA haplotype significantly inhibited luciferase activity compared with the H1: TCG haplotype. In conclusion, we identified a suppressive role for TG in the genome-wide association between LIPC and HDL-C. A functional haplotype of hepatic lipase may reduce HDL-C levels and is suppressed by TG.
Effects of mitoTALENs-Directed Double-Strand Breaks on Plant Mitochondrial Genomes
Authors: Arimura Shin-ichi
Published: January 2021

Mitochondrial genomes in flowering plants differ from those in animals and yeasts in several ways, including having large and variable sizes, circular, linear and branched structures, long repeat sequences that participate in homologous recombinations, and variable genes orders, even within a species. Understanding these differences has been hampered by a lack of genetic methods for transforming plant mitochondrial genomes. We recently succeeded in disrupting targeted genes in mitochondrial genomes by mitochondria-targeted transcription activator-like effector nucleases (mitoTALENs) in rice, rapeseed, and Arabidopsis. Double-strand breaks created by mitoTALENs were repaired not by non-homologous end-joining (NHEJ) but by homologous recombination (HR) between repeats near and far from the target sites, resulting in new genomic structures with large deletions and different configurations. On the other hand, in mammals, TALENs-induced DSBs cause small insertions or deletions in nuclear genomes and degradation of mitochondrial genomes. These results suggest that the mitochondrial and nuclear genomes of plants and mammals have distinct mechanisms for responding to naturally occurring DSBs. The different responses appear to be well suited to differences in size and copy numbers of each genome.
Let’s Not Joke about It Too Much! Exposure to COVID-19 Messaging, Attitudes and Protective Behavioral Intentions
Authors: Curșeu Petru L. , Coman Andra D. , Fodor Oana C. , Rațiu Lucia , Panchenko Anton
Published: January 2021

Our study evaluates the role of exposure to COVID-19 messaging in negativity towards COVID-19 and the intentions to engage in protective behaviors. Building on the theory of planned behavior (TPB), we derive a mediation model and test it in a sample of 737 participants (556 Romanians and 181 Kazakhs). The exposure to general information concerning COVID-19 positively predicts negative attitudes, negative emotions and the emergence of subjective norms in relation to COVID-19, as well as the likelihood of engaging in protective behavioral intentions. The exposure to humoristic communication, however, diluted the positive association between exposure to general information and negative attitudes, as well as negative emotions. The results support the overall predictions of the TPB and report positive associations between negative attitudes towards COVID-19, subjective norms and behavioral control on the one hand, and protective behavioral intentions on the other. Negative emotions significantly predict the likelihood of engaging in protective behaviors. Our results also show that older respondents tend to develop more negative attitudes towards COVID-19, yet they do not report stronger intentions to engage in protective behaviors than younger respondents. An important emergent result shows that time lapse seems to increase negativity towards COVID-19, yet it does not directly increase the likelihood of engaging in protective behaviors. Implications for public health communication related to COVID-19 are discussed.
Hydrological Response of Natural Mediterranean Watersheds to Forest Fires
Authors: Soulis Konstantinos X. , Generali Konstantina Amalia , Papadaki Christina , Theodoropoulos Christos , Psomiadis Emmanouil
Published: January 2021

Fires are common in forested Mediterranean-climate watersheds. Forest fires cause abrupt land use/cover (LULC) changes affecting soil properties and hydrological processes within and across watersheds. A major forest fire in Attica, Greece, that affected the Lykorrema stream experimental watershed provided the opportunity for an in-depth study of the impact of forest fires on the hydrological balance of natural Mediterranean watersheds. To this end, detailed hydrometeorological data recorded for five years before and for five years after the fire incidence were utilized. SWAT model was also used to consider the potential influence of meteorological conditions temporal variability on the results of the analysis. Specifically, SWAT model was parameterized calibrated and validated for the pre-fire and the post-fire conditions using the corresponding detailed hydrometeorological data for the respective periods. Then the two versions of the model were applied for the entire period providing comprehensive time series for all the flows and storages in the studied watershed. In this way, the post-fire LULC and soil properties changes were the only influencing factors driving the alterations in the hydrological balance allowing an impartial comparison. The obtained results highlighted the considerable impact of forest fires on the watersheds’ hydrological functioning. Specifically, the maximum direct runoff depths and the maximum flow rates were substantially higher in the post-fire conditions. In contrast, actual evapotranspiration was reduced, when the effect of fire was considered. The obtained results indicate that the altered post-fire LULC and soil properties are major drivers of the watershed’s hydrological balance changes. SWAT model performed sufficiently well for both the pre- and post-fire conditions and provided a deeper insight into the impact of forest fires on the hydrological functioning of natural Mediterranean watersheds.
Drug Reaction with Eosinophilia and Systemic Symptoms: A Complex Interplay between Drug, T Cells, and Herpesviridae
Authors: Ganeshanandan Luckshman , Lucas Michaela
Published: January 2021

Drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome, also known as drug induced hypersensitivity (DiHS) syndrome is a severe delayed hypersensitivity reaction with potentially fatal consequences. Whilst recognised as T cell-mediated, our understanding of the immunopathogenesis of this syndrome remains incomplete. Here, we discuss models of DRESS, including the role of human leukocyte antigen (HLA) and how observations derived from new molecular techniques adopted in key studies have informed our mechanism-based understanding of the central role of Herpesviridae reactivation and heterologous immunity in these disorders.
Neovascular Macular Degeneration: A Review of Etiology, Risk Factors, and Recent Advances in Research and Therapy
Authors: Pugazhendhi Arunbalaji , Hubbell Margaret , Jairam Pooja , Ambati Balamurali
Published: January 2021

Neovascular age-related macular degeneration (exudative or wet AMD) is a prevalent, progressive retinal degenerative macular disease that is characterized by neovascularization of the choroid, mainly affecting the elderly population causing gradual vision impairment. Risk factors such as age, race, genetics, iris color, smoking, drinking, BMI, and diet all play a part in nvAMD’s progression, with anti-vascular endothelial growth factor (anti-VEGF) therapy being the mainstay of treatment. Current therapeutic advancements slow the progression of the disease but do not cure or reverse its course. Newer therapies such as gene therapies, Rho-kinase inhibitors, and levodopa offer potential new targets for treatment.
Acknowledgment to Reviewers of IJTPP in 2020
Authors:   IJTPP Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that IJTPP maintains its standards for the high quality of its published papers [...]
Acknowledgment to Reviewers of Informatics in 2020
Authors:   Informatics Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Informatics maintains its standards for the high quality of its published papers [...]
Developing Pavement Marking Management Systems: A Theoretical Model Framework Based on the Experiences of the US Transportation Agencies
Authors: Sassani Alireza , Smadi Omar , Hawkins Neal
Published: January 2021

Pavement markings are essential elements of transportation infrastructure with critical impacts on safety and mobility. They provide road users with the necessary information to adjust driving behavior or make calculated decisions about commuting. The visibility of pavement markings for drivers can be the boundary between a safe trip and a disastrous accident. Consequently, transportation agencies at the local or national levels allocate sizeable budgets to upkeep the pavement markings under their jurisdiction. Infrastructure asset management systems (IAMS) are often biased toward high-capital-cost assets such as pavements and bridges, not providing structured asset management (AM) plans for low-cost assets such as pavement markings. However, recent advances in transportation asset management (TAM) have promoted an integrated approach involving the pavement marking management system (PMMS). A PMMS brings all data items and processes under a comprehensive AM plan and enables managing pavement markings more efficiently. Pavement marking operations depend on location, conditions, and AM policies, highly diversifying the pavement marking management practices among agencies and making it difficult to create a holistic image of the system. Most of the available resources for pavement marking management focus on practices instead of strategies. Therefore, there is a lack of comprehensive guidelines and model frameworks for developing PMMS. This study utilizes the existing body of knowledge to build a guideline for developing and implementing PMMS. First, by adapting the core AM concepts to pavement marking management, a model framework for PMMS is created, and the building blocks and elements of the framework are introduced. Then, the caveats and practical points in PMMS implementation are discussed based on the US transportation agencies’ experiences and the relevant literature. This guideline is aspired to facilitate PMMS development for the agencies and pave the way for future pavement marking management tools and databases.
Tomato Chlorosis Virus Infection Facilitates Bemisia tabaci MED Reproduction by Elevating Vitellogenin Expression
Authors: Huang Liping , Shi Xiaobin , Shi Jizhe , Zhang Zhuo , Fang Yong , Zhang Zhanhong , Pan Qiuyi , Zheng Limin , Gao Yang , Zhang Deyong , Tan Xinqiu , Liu Yong , Zhou Xuguo
Published: January 2021

Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression.
The Analysis of Selected Morphological and Hemodynamic Parameters of the Venous System and Their Presumable Impact on the Risk of Recurrence after Varicose Vein Treatment
Authors: Szary Cezary , Wilczko Justyna , Plucinska Dominika , Pachuta Anna , Napierala Marcin , Bodziony Anna , Zawadzki Michal , Leszczynski Jerzy , Galazka Zbigniew , Grzela Tomasz
Published: January 2021

Introduction: The current treatment of venous disease is focused on reflux elimination in main venous trunks, especially in the saphenous vein. However, a high recurrence rate, independent of the method of treatment, suggests that the reason of low effectiveness may be due to a strategy focused on symptoms, without considering their origin. Method: The aim of study was the comparison of retrospective data from 535 women with venous disease, either after treatment (n = 183) or not treated before (n = 352). The analysis concerned clinical symptoms and the results of the extended diagnostics, including the examination of the lower limb, pelvic and abdominal veins either using duplex-doppler ultrasound as well as venography with computed tomography or magnetic resonance. Results: The comparison of selected venous system parameters revealed more advanced disease progression in previously treated patients, compared to non-treated individuals (e.g., ipsi- or bilateral incompetence of sapheno-phemoral junction—29.5% vs. 20.4%, at P < 0.05 and 13.6% vs. 7.7% at P < 0.05, respectively). This difference could be explained by post-treatment alterations in the venous system, an older age and the higher number of pregnancies in the recurrence group. However, both groups did not differ in regards to the symptoms of pelvic venous insufficiency or the frequency of relevant variants/abnormalities in venous system. Conclusions: Based on the aforementioned findings, we postulate the revision of treatment strategy, which should consider abdominal and pelvic veins as the source of reflux in many female subjects.