bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Temporal Data Fusion in MS and SAR Images Using the Dynamic Time Warping Method for Paddy Rice Classification
Authors: Lei Tsu Chiang , Wan Shiuan , Wu You Cheng , Wang Hsin-Ping , Hsieh Chia-Wen
Published: January 2022

This study employed a data fusion method to extract the high-similarity time series feature index of a dataset through the integration of MS (Multi-Spectrum) and SAR (Synthetic Aperture Radar) images. The farmlands are divided into small pieces that consider the different behaviors of farmers for their planting contents in Taiwan. Hence, the conventional image classification process cannot produce good outcomes. The crop phenological information will be a core factor to multi-period image data. Accordingly, the study intends to resolve the previous problem by using three different SPOT6 satellite images and nine Sentinel-1A synthetic aperture radar images, which were used to calculate features such as texture and indicator information, in 2019. Considering that a Dynamic Time Warping (DTW) index (i) can integrate different image data sources, (ii) can integrate data of different lengths, and (iii) can generate information with time characteristics, this type of index can resolve certain classification problems with long-term crop classification and monitoring. More specifically, this study used the time series data analysis of DTW to produce “multi-scale time series feature similarity indicators”. We used three approaches (Support Vector Machine, Neural Network, and Decision Tree) to classify paddy patches into two groups: (a) the first group did not apply a DTW index, and (b) the second group extracted conflict predicted data from (a) to apply a DTW index. The outcomes from the second group performed better than the first group in regard to overall accuracy (OA) and kappa. Among those classifiers, the Neural Network approach had the largest improvement of OA and kappa from 89.51, 0.66 to 92.63, 0.74, respectively. The rest of the two classifiers also showed progress. The best performance of classification results was obtained from the Decision Tree of 94.71, 0.81. Observing the outcomes, the interference effects of the image were resolved successfully by various image problems using the spectral image and radar image for paddy rice classification. The overall accuracy and kappa showed improvement, and the maximum kappa was enhanced by about 8%. The classification performance was improved by considering the DTW index.
ScRpb4, Encoding an RNA Polymerase Subunit from Sugarcane, Is Ubiquitously Expressed and Resilient to Changes in Response to Stress Conditions
Authors: Kim Taehoon , Dias Fábio Ometto , Gentile Agustina , Menossi Marcelo , Begcy Kevin
Published: January 2022

RNA polymerase II is an essential multiprotein complex that transcribes thousands of genes, being a fundamental component of the transcription initiation complex. In eukaryotes, RNA polymerase II is formed by a 10-multisubunit conserved core complex, and two additional peripheral subunits, Rpb4 and Rpb7, form the Rpb4/7 subcomplex. Although transcription is vital for cell and organismal viability, little is known about the transcription initiation complex in sugarcane. An initial characterization of the sugarcane RNA polymerase subunit IV (ScRpb4) was performed. Our results demonstrate that ScRpb4 is evolutionarily conserved across kingdoms. At the molecular level, ScRpb4 expression was found in vegetative and reproductive tissues. Furthermore, the expression of ScRpb4 remained stable under various stress conditions, most likely to ensure a proper transcriptional response. Optimal conditions to express ScRpb4 in vitro for further studies were also identified. In this study, an initial characterization of the sugarcane polymerase II subunit IV is presented. Our results open the window to more specific experiments to study ScRpb4 function, for instance, crystal structure determination and pull-down assays as well as their function under biotic and abiotic stresses.
Pre-COVID-19 Organic Market in the European Union—Focus on the Czech, German, and Slovak Markets
Authors: Rojík Stanislav , Zámková Martina , Chalupová Martina , Pilař Ladislav , Prokop Martin , Stolín Radek , Malec Karel , Appiah-Kubi Seth Nana Kwame , Maitah Mansoor , Dziekański Paweł , Prus Piotr
Published: January 2022

This article compares attitudes to buying organic food in selected countries in Central Europe. The current research was conducted in 2019 on a total sample of 2800 respondents in the Czech Republic, Slovakia (Central Europe, with a relatively new organic food market), and Germany (a traditional Western Europe country with a mature food market). The study results demonstrate significant differences between the three selected markets. The product quality is the most important for German consumers. Slovak consumers consider organic food to be the least recognizable and least promoted of all the regions surveyed, and they are also the least likely to encounter targeted advertising for organic products. Germany is the country where most respondents regularly or occasionally buy organic food. In Slovakia, an interesting finding is the highest proportion of respondents who do not buy organic food at all. Czech respondents often buy organic products in specialized shops and like to grow organic products themselves. The results also suggest that Slovak consumers slightly more often prefer foreign organic products to the local ones, whereas consumers in Germany select regional organic products more often and prefer to buy regional products at farmers’ markets.
Impacts of Slow-Release Nitrogen Fertilizer Rates on the Morpho-Physiological Traits, Yield, and Nitrogen Use Efficiency of Rice under Different Water Regimes
Authors: AL Aasmi Alaa , Li Jiuhao , Hamoud Yousef Alhaj , Lan Yubin , Alordzinu Kelvin Edom , Appiah Sadick Amoakohene , Shaghaleh Hiba , Sheteiwy Mohamed , Wang Hao , Qiao Songyang , Yu Chaoran
Published: January 2022

The efficient use of water and fertilizer is vital for optimizing plant growth and yield in rice production. To achieve sustainable rice production and resource management, the ways in which applied water and nitrogen affect the root and shoot morpho-physiology, as well as yield, must be understood. In this study, a pot experiment was conducted to investigate the effects of slow-release nitrogen fertilizer (sulfur-coated urea) application at three levels (light nitrogen (NL), medium nitrogen (NM), and heavy nitrogen (NH)) on the growth, yield, and nitrogen use efficiency (NUE) of rice grown under three water regimes (wetting and soil saturation (WSS), wetting and moderate drying (WMD) and wetting and severe drying (WSD)). The results revealed that differences in water regimes and fertilizer rates led to significant differences in the roots, shoots, yield, and NUE of rice. Increasing the N dosage by 5% enhanced the root and biomass production by 16% in comparison with that of the other groups. The NH×WSS treatment produced the greatest root length, weight, density, active absorption, and oxidation. However, the integration of WSS × NL generated the maximum value of nitrogen apparent recovery efficiency (63.1% to 67.6%) and the greatest value of nitrogen partial factor productivity (39.9 g g−1 to 41.13 g g−1). Transmission electron microscopy (TEM) images showed that plants grown under high and medium nitrogen fertilizer rates with WSS had improved leaf mesophyll structure with normal starch grains, clear cell walls, and well-developed chloroplasts with tidy and well-arranged thylakoids. These results show that TEM images are useful for characterizing the nitrogen and water status of leaves in the sub-micrometer range and providing specific information regarding the leaf microstructure. The findings of this study suggest that the application of NH×WSS can produce improvements in growth traits and increase rice yield; however, the NL×WSS treatment led to greater NUE, and the authors recommend its usage in rice agriculture.
Guanidinoacetic Acid Supplementation Promotes Skeletal Muscle Fiber Type Transformation from Fast-to-Slow-Twitch via Increasing the PPARGC1A Based Mitochondrial Function and CaN/NFAT Pathway in Finishing Pigs
Authors: Li Jingzheng , Li Jiaolong , Zhang Lin , Xing Tong , Jiang Yun , Gao Feng
Published: January 2022

Guanidinoacetic acid can improve pork quality. Previous studies have demonstrated that pork quality is closely linked to the muscle fiber type mediated by PPARGC1A. Therefore, this study aimed to evaluate the influence of dietary GAA supplementation on the skeletal muscle fiber type transformation. A total of 180 healthy Duroc × Landrace × Meishan cross castrated male pigs with a similar average weight (90 ± 1.5 kg) were randomly divided into three treatments with five replicates per treatment and 12 pigs per replicate, including a GAA-free basal diet and basal diet with 0.05% or 0.10% GAA for 15 days. Our results showed that 0.10% GAA supplementation increased the contents of Ca2+ in sarcoplasm (p < 0.05). Compared with the control group, both GAA supplementation groups upregulated the expression of Troponin I-ss (p < 0.05), and 0.10% GAA supplementation downregulated the expression of Troponin T3 (p < 0.05). GAA supplementation increased the expression of peroxisome proliferator activated receptor-γ coactivator-1alpha (PPARGC1A) (p < 0.05), and further upregulated the mitochondrial transcription factor A (TFAM), increased the level of membrane potential, and the activities of mitochondrial respiratory chain complex I, III (p < 0.05). The 0.10% GAA supplementation upregulated the protein expression of calcineurin catalytic subunit α (CnAα) and nuclear factor of activated T cells (NFATc1) (p < 0.05). Overall, dietary GAA supplementation promotes skeletal muscle fiber types transformation from fast-to-slow-twitch via increasing the PPARGC1A based mitochondrial function and the activation of CaN/NFAT pathway in finishing pigs.
Source and Accumulation of Soil Carbon along Catena Toposequences over 12,000 Years in Three Semi-Natural Miscanthus sinensis Grasslands in Japan
Authors: Howlett David S. , Stewart J. Ryan , Inoue Jun , Saito Masanori , Lee DoKyoung , Wang Hong , Yamada Toshihiko , Nishiwaki Aya , Fernández Fabián G. , Toma Yo
Published: January 2022

Miscanthus-dominated semi-natural grasslands in Japan appear to store considerable amounts of soil C. To estimate the long-term effect of Miscanthus vegetation on the accumulation of soil carbon by soil biota degradation in its native range, we measured total soil C from the surface to a 1.2 m depth along a catena toposequence in three annually burned grasslands in Japan: Kawatabi, Soni, and Aso. Soil C stock was estimated using a radiocarbon age and depth model, resulting in a net soil C accumulation rate in the soil. C4-plant contribution to soil C accumulation was further estimated by δ13C of soil C. The range of total soil C varied among the sites (i.e., Kawatabi: 379–638 Mg, Soni: 249–484, and Aso: 372–408 Mg C ha−1). Catena position was a significant factor at Kawatabi and Soni, where the toe slope soil C accumulation exceeded that of the summit. The soil C accumulation rate of the whole horizon in the grasslands, derived C mainly from C4 plant species, was 0.05 ± 0.02 (Average ± SE), 0.04 ± 0.00, and 0.24 ± 0.04 Mg C ha−1 yr−1 in Kawatabi, Soni, and Aso, respectively. Potential exists for long-term sequestration of C under M. sinensis, but the difference in the C accumulation rate can be influenced by the catena position and the amount of vegetation.
Mycorrhizal Fungi Inoculation Improves Capparis spinosa’s Yield, Nutrient Uptake and Photosynthetic Efficiency under Water Deficit
Authors: Bouskout Mohammed , Bourhia Mohammed , Al Feddy Mohamed Najib , Dounas Hanane , Salamatullah Ahmad Mohammad , Soufan Walid , Nafidi Hiba-Allah , Ouahmane Lahcen
Published: January 2022

Agricultural yields are under constant jeopardy as climate change and abiotic pressures spread worldwide. Using rhizospheric microbes as biostimulants/biofertilizers is one of the best ways to improve agro-agriculture in the face of these things. The purpose of this experiment was to investigate whether a native arbuscular mycorrhizal fungi inoculum (AMF-complex) might improve caper (Capparis spinosa) seedlings’ nutritional status, their morphological/growth performance and photosynthetic efficiency under water-deficit stress (WDS). Thus, caper plantlets inoculated with or without an AMF complex (+AMF and −AMF, respectively) were grown under three gradually increasing WDS regimes, i.e., 75, 50 and 25% of field capacity (FC). Overall, measurements of morphological traits, biomass production and nutrient uptake (particularly P, K+, Mg2+, Fe2+ and Zn2+) showed that mycorrhizal fungi inoculation increased these variables significantly, notably in moderate and severe WDS conditions. The increased WDS levels reduced the photochemical efficiency indices (Fv/Fm and Fv/Fo) in −AMF plants, while AMF-complex application significantly augmented these parameters. Furthermore, the photosynthetic pigments content was substantially higher in +AMF seedlings than −AMF controls at all the WDS levels. Favorably, at 25% FC, AMF-colonized plants produce approximately twice as many carotenoids as non-colonized ones. In conclusion, AMF inoculation seems to be a powerful eco-engineering strategy for improving the caper seedling growth rate and drought tolerance in harsh environments.
Apple Autotetraploids—Phenotypic Characterisation and Response to Drought Stress
Authors: Wójcik Danuta , Marat Monika , Marasek-Ciołakowska Agnieszka , Klamkowski Krzysztof , Buler Zbigniew , Podwyszyńska Małgorzata , Tomczyk Przemysław Piotr , Wójcik Katarzyna , Treder Waldemar , Filipczak Jacek
Published: January 2022

Polyploidization is an important source of variability for plant breeding. Polyploids are often characterised by increased resistance to biotic and abiotic stresses. Since drought and pathogen attack are the main threats to apple cultivation, obtaining new sources of resistance is an important issue for apple breeding. The newly obtained autotetraploid clones of apple cv. ‘Redchief’ showed superior resistance to fire blight. The aim of the presented research was the in-depth phenotypic characterisation of ‘Redchief’ tetraploids and assessment of their response to drought at the physiological and genetic level. The growth of own-rooted five-year-old trees of ‘Redchief’ tetraploids was poor compared with diploids; all growth parameters—the number and length of current season shoots, the total length of current season shoots per tree and the cross-section area of the trunk—were reduced in tetraploid clones. Grafting on M9 rootstock improved the growth characteristics of ‘Redchief’ tetraploids. Compared with diploid plants, the leaves of tetraploids were thicker, with altered shape, higher chlorophyll content, and larger stomata, but the stomatal density decreased. The leaf anatomical structure of tetraploids was changed, the adaxial and abaxial epidermis and both types of mesophyll were significantly thicker than in diploids. Moreover, the pollen grains of tetraploids were larger, but their viability and germination were reduced. Under conditions of limited water supply, the reduction in growth parameters was smaller and the physiological parameters were higher in the ‘Redchief’ tetraploid clone 4x-25 than in diploid plants. The expression of APX gene was higher in tetraploids than in diploids 15 days after drought stress induction. The results suggest the enhanced drought tolerance of the studied ‘Redchief’ autotetraploid clone compared with its diploid counterpart.
Antimicrobial Stewardship Program: Reducing Antibiotic’s Spectrum of Activity Is not the Solution to Limit the Emergence of Multidrug-Resistant Bacteria
Authors: Saliba Rindala , Mizrahi Assaf , Gauthier Péan de Ponfilly , Alban Le Monnier , Zahar Jean-Ralph , Pilmis Benoît
Published: January 2022

Overconsumption of antibiotics in hospitals has led to policy implementation, including the control of antibiotic prescriptions. The impact of these policies on the evolution of antimicrobial resistance remains uncertain. In this work, we review the possible limits of such policies and focus on the need for a more efficient approach. Establishing a causal relationship between the introduction of new antibiotics and the emergence of new resistance mechanisms is difficult. Several studies have demonstrated that many resistance mechanisms existed before the discovery of antibiotics. Overconsumption of antibiotics has worsened the phenomenon of resistance. Antibiotics are responsible for intestinal dysbiosis, which is suspected of being the source of bacterial resistance. The complexity of the intestinal microbiota composition, the impact of the pharmacokinetic properties of antibiotics, and the multiplicity of other factors involved in the acquisition and emergence of multidrug-resistant organisms, lead us to think that de-escalation, in the absence of studies proving its effectiveness, is not the solution to limiting the spread of multidrug-resistant organisms. More studies are needed to clarify the ecological risk caused by different antibiotic classes. In the meantime, we need to concentrate our efforts on limiting antibiotic prescriptions to patients who really need it, and work on reducing the duration of these treatments.
Antifungal Activity of Natural Compounds vs. Candida spp.: A Mixture of Cinnamaldehyde and Eugenol Shows Promising In Vitro Results
Authors: Saracino Ilaria Maria , Foschi Claudio , Pavoni Matteo , Spigarelli Renato , Valerii Maria Chiara , Spisni Enzo
Published: January 2022

Candida spp. are commensal organisms of the skin, mucous membranes, gastrointestinal tract, blood, and vagina of animals and humans. In recent decades, the incidence of human fungal infections has increased, with Candida spp. (mainly C. albicans) infections being the most frequent, and the treatment of fungal infections is still a clinical challenge. Colonization of the human gastrointestinal tract by Candida spp. is significant because infections (e.g., candidemia and vulvovaginal candidiasis) frequently arise from commensal microorganisms. The aim of this study was to test in vitro the antifungal activity and the eventual synergistic effect of five pure components of essential oils: cinnamaldehyde, α-pinene, limonene, eucalyptol, and eugenol. These compounds were tested on 18 Candida strains (15 C. albicans, 2 C. glabrata, and 1 C. lusitaniae) derived from a culture collection of vaginal clinical strains. Methods: Fungistatic activity was evaluated using the disk diffusion method. For fungicidal activity, microdilution and time–kill curve protocols were set up. The checkerboard method was chosen to evaluate a possible synergistic effect of these compounds when mixed. Results: Cinnamaldehyde and eugenol gave the best results, inhibiting all the Candida strains and showing a highly additive effect (FICI 0.625). The cinnamaldehyde inhibition zone (IZ), MIC, and MFC means were 69 mm, 50.05 mg/L, and 109.26 mg/L respectively. Cinnamaldehyde led to the total loss of viable Candida cells within 4 h. Eugenol IZ, MIC, and MFC means were 35.2 mm, 455.42 mg/L, and 690.09 mg/L, respectively. Eugenol led to the total loss of viable fungal cells within 1 h. Treatment with α-pinene inhibited 88.9% of Candida strains, with an IZ mean of 21.2 mm, a MIC mean of 195.41 mg/L, and a MFC mean of 251.27 mg/L; this compound led to the total loss of viable fungal cells only after 24 h. Limonene inhibited only 33.3% of the tested strains and eucalyptol did not produce an inhibition halo, so these compounds were not tested further. Conclusions: These characteristics, together with the well-known safety of cinnamaldehyde and eugenol for human use, make these two natural compounds the perfect candidates for the treatment of candidiasis. This was a pilot study, the purpose of which was to evaluate the best composition of a mixture to be used against intestinal and vulvovaginal candidiasis; in vivo studies are needed to confirm these very encouraging results.
The Overlooked Issue of Outpatient Combination Antibiotic Prescribing in Low- and Middle-Income Countries: An Example from Syria
Authors: Tomas Ana , Aljadeeah Saleh
Published: January 2022

This study aimed to determine and describe the prevalence of combination antibiotics dispensed in outpatients with health insurance in Syria. Data on all dispensed medicines between June 2018 and May 2019 for 81,314 adults were obtained, and medicines belonging to the J01 group of the World Health Organization (WHO) anatomical therapeutic classification (ATC) were included in the analysis. Prescriptions were stratified according to the number of antibiotics, age, and sex. Antibiotic utilization was expressed as the number of prescriptions per 1000 persons per year. Out of 59,404 prescriptions for antibiotics, 14.98% contained antibiotic combinations, distributed to 22.49% of the patients. The prevalence of dispensing antibiotic combinations was higher in female patients (23.00%), and the youngest (18–30 years, 26.19%) and oldest age groups (>70 years, 25.19%). The antibiotics most commonly combined were co-amoxiclav, second- and third-generation cephalosporins, and macrolides. Over 60% of the combinations contained ceftriaxone alone or in combination with sulbactam. The present study shows an alarmingly widespread prescription of antibiotic combinations, posing a risk to global health by promoting resistance development.
Innovative Perspectives on Biofilm Interactions in Poultry Drinking Water Systems and Veterinary Antibiotics Used Worldwide
Authors: Hahne Friederike , Jensch Simon , Hamscher Gerd , Meißner Jessica , Kietzmann Manfred , Kemper Nicole , Schulz Jochen , Mateus-Vargas Rafael H.
Published: January 2022

Prudent use of antibiotics in livestock is widely considered to be important to prevent antibiotic resistance. This study aimed to evaluate the interactions between biofilms and veterinary antibiotics in therapeutic concentrations administrated via drinking water through a standardized experimental setup. In this context, two biofilms formed by pseudomonads (Pseudomonas (P.) aeruginosa or P. fluorescens) and a susceptible Escherichia (E.) coli strain were developed in a nutrient-poor medium on the inner surface of polyvinyl chloride pipe pieces. Subsequently, developing biofilms were exposed to sulfadiazine/trimethoprim (SDZ/TMP) or tylosin A (TYL A) in dosages recommended for application in drinking water for 5 or 7 days, respectively. Various interactions were detected between biofilms and antibiotics. Microbiological examinations revealed that only TYL A reduced the number of bacteria on the surface of the pipes. Additionally, susceptible E. coli survived both antibiotic treatments without observable changes in the minimum inhibitory concentration to 13 relevant antibiotics. Furthermore, as demonstrated by HPLC-UV, the dynamics of SDZ/TMP and TYL A in liquid media differed between the biofilms of both pseudomonads over the exposure period. We conclude that this approach represents an innovative step toward the effective evaluation of safe veterinary antibiotic use.
In Vitro and In Silico Approaches for the Evaluation of Antimicrobial Activity, Time-Kill Kinetics, and Anti-Biofilm Potential of Thymoquinone (2-Methyl-5-propan-2-ylcyclohexa-2,5-diene-1,4-dione) against Selected Human Pathogens
Authors: Qureshi Kamal A. , Imtiaz Mahrukh , Parvez Adil , Rai Pankaj K. , Jaremko Mariusz , Emwas Abdul-Hamid , Bholay Avinash D. , Fatmi Muhammad Qaiser
Published: January 2022

Thymoquinone (2-methyl-5-propan-2-ylcyclohexa-2,5-diene-1,4-dione; TQ), a principal bioactive phytoconstituent of Nigella sativa essential oil, has been reported to have high antimicrobial potential. Thus, the current study evaluated TQ’s antimicrobial potential against a range of selected human pathogens using in vitro assays, including time-kill kinetics and anti-biofilm activity. In silico molecular docking of TQ against several antimicrobial target proteins and a detailed intermolecular interaction analysis was performed, including binding energies and docking feasibility. Of the tested bacteria and fungi, S. epidermidis ATCC 12228 and Candida albicans ATCC 10231 were the most susceptible to TQ, with 50.3 ± 0.3 mm and 21.1 ± 0.1 mm zones of inhibition, respectively. Minimum inhibitory concentration (MIC) values of TQ are in the range of 12.5–50 µg/mL, while minimum biocidal concentration (MBC) values are in the range of 25–100 µg/mL against the tested organisms. Time-kill kinetics of TQ revealed that the killing time for the tested bacteria is in the range of 1–6 h with the MBC of TQ. Anti-biofilm activity results demonstrate that the minimum biofilm inhibitory concentration (MBIC) values of TQ are in the range of 25–50 µg/mL, while the minimum biofilm eradication concentration (MBEC) values are in the range of 25–100 µg/mL, for the tested bacteria. In silico molecular docking studies revealed four preferred antibacterial and antifungal target proteins for TQ: D-alanyl-D-alanine synthetase (Ddl) from Thermus thermophilus, transcriptional regulator qacR from Staphylococcus aureus, N-myristoyltransferase from Candida albicans, and NADPH-dependent D-xylose reductase from Candida tenuis. In contrast, the nitroreductase family protein from Bacillus cereus and spore coat polysaccharide biosynthesis protein from Bacillus subtilis and UDP-N-acetylglucosamine pyrophosphorylase from Aspergillus fumigatus are the least preferred antibacterial and antifungal target proteins for TQ, respectively. Molecular dynamics (MD) simulations revealed that TQ could bind to all four target proteins, with Ddl and NADPH-dependent D-xylose reductase being the most efficient. Our findings corroborate TQ’s high antimicrobial potential, suggesting it may be a promising drug candidate for multi-drug resistant (MDR) pathogens, notably Gram-positive bacteria and Candida albicans.
Knowledge, Attitudes, and Common Practices of Livestock and Poultry Veterinary Practitioners Regarding the AMU and AMR in Bangladesh
Authors: Kalam Md. Abul , Rahman Md. Sahidur , Alim Md. Abdul , Shano Shahanaj , Afrose Sharmin , Jalal Faruk Ahmed , Akter Samira , Khan Shahneaz Ali , Islam Md. Mazharul , Uddin Md Bashir , Islam Ariful , Magalhães Ricardo J. Soares , Hassan Mohammad Mahmudul
Published: January 2022

Current evidence indicates that more than half of all antimicrobials are used in the animal food-producing sector, which is considered a significant risk factor for the development, spread, and existence of antimicrobial resistance (AMR) pathogens in animals, humans, and the environment. Among other factors, clinical etiology and the level of knowledge, attitudes, and practices (KAP) of veterinarians are thought to be responsible for inappropriate prescriptions in the animal-source protein production sector in lower-resource settings like Bangladesh. We performed this cross-sectional study to assess factors associated with veterinarians’ antimicrobial prescription behavior and their KAP on antimicrobial use (AMU) and AMR in Bangladesh. Exploratory and multivariate logistic models were used to describe an association between knowledge, attitudes, and practices of AMU and AMR and demographic characteristics of veterinarians. The results demonstrated that when selecting an antimicrobial, there was no to minimal influence of culture and susceptibility tests and patients’ AMU history but moderate to high influence of the farmer’s economic condition and drug instructions among the veterinarians. The results also demonstrated that more than half of the veterinarians had correct KAP regarding AMU and AMR, while the rest had moderate or lower levels of KAP. The factor score analysis revealed that age, level of education, years of experience, gender, and previous training on AMU and AMR were the key influencing factors in their level of KAP. Adjusted logistic regression analysis showed that respondents’ age, current workplace, and previous training on AMU and AMR had a positive association with increased KAP. Considering the results, it is imperative to include AMR issues on vet curricula, and to provide post-education training, awareness campaigns, easy access to, and dissemination of AMR resources. Increasing the veterinary services to the outreach areas of the country and motivating veterinarians to follow the national AMR guidelines could be some other potential solutions to tackle the over-prescriptions of antimicrobials.
Role of Bioaerosols on the Short-Distance Transmission of Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus (MRSA) in a Chicken Farm Environment
Authors: Hsu Bing-Mu , Chen Jung-Sheng , Hsu Gwo-Jong , Koner Suprokash , Nagarajan Viji , Tsai Hsin-Chi
Published: January 2022

Methicillin-resistant Staphylococcus aureus (MRSA) is a dynamic and tenacious pathogenic bacterium which is prevalent in livestock farming environments. This study investigated the possibility of MRSA spread via bioaerosol transmission from an indoor chicken farm environment to outdoors downwind (up to 50 m). The concentration of total airborne bacteria colony formation units (CFUs) was decreased with increasing sampling distance ranging from 9.18 × 101 to 3.67 × 103 per air volume (m3). Among the 21 MRSA isolates, 15 were isolated from indoor chicken sheds and exposure square areas, whereas 6 were isolated from downwind bioaerosol samples. Molecular characterization revealed that all of them carried the staphylococcal cassette chromosome mec (SCCmec) VIII, and they were remarkably linked with the hospital-associated MRSA group. Spa typing analysis determined that all MRSA isolates belonged to spa type t002. Virulence analysis showed that 100% of total isolates possessed exfoliative toxin A (eta), whereas 38.09% and 23.80% strains carried exfoliative toxin B (etb) and enterotoxin A (entA). Additionally, all of these MRSA isolates carried multidrug resistance properties and showed their resistance against chloramphenicol, ciprofloxacin, clindamycin, tetracycline, and erythromycin. In addition, chi-squared statistical analysis displayed a significant distributional relationship of gene phenotypes between MRSA isolates from chicken farm indoor and downwind bioaerosol samples. The results of this study revealed that chicken farm indoor air might act as a hotspot of MRSA local community-level outbreak, wherein the short-distance dispersal of MRSA could be supported by bioaerosols.
Protection against Amyloid-β Oligomer Neurotoxicity by Small Molecules with Antioxidative Properties: Potential for the Prevention of Alzheimer’s Disease Dementia
Authors: Araki Wataru , Kametani Fuyuki
Published: January 2022

Soluble oligomeric assemblies of amyloid β-protein (Aβ), called Aβ oligomers (AβOs), have been recognized as primary pathogenetic factors in the molecular pathology of Alzheimer’s disease (AD). AβOs exert neurotoxicity and synaptotoxicity and play a critical role in the pathological progression of AD by aggravating oxidative and synaptic disturbances and tau abnormalities. As such, they are important therapeutic targets. From a therapeutic standpoint, it is not only important to clear AβOs or prevent their formation, it is also beneficial to reduce their neurotoxicity. In this regard, recent studies have reported that small molecules, most with antioxidative properties, show promise as therapeutic agents for reducing the neurotoxicity of AβOs. In this mini-review, we briefly review the significance of AβOs and oxidative stress in AD and summarize studies on small molecules with AβO-neurotoxicity-reducing effects. We also discuss mechanisms underlying the effects of these compounds against AβO neurotoxicity as well as their potential as drug candidates for the prevention and treatment of AD.
Generation of Hydrogen Peroxide and Downstream Protein Kinase D1 Signaling Is a Common Feature of Inducers of Pancreatic Acinar-to-Ductal Metaplasia
Authors: Döppler Heike R. , Liou Geou-Yarh , Storz Peter
Published: January 2022

Pancreatic acinar-to-ductal metaplasia (ADM) is a reversible process that occurs after pancreatic injury, but becomes permanent and leads to pancreatic lesions in the presence of an oncogenic mutation in KRAS,. While inflammatory macrophage-secreted chemokines, growth factors that activate epidermal growth factor receptor (EGFR) and oncogenic KRAS have been implicated in the induction of ADM, it is currently unclear whether a common underlying signaling mechanism exists that drives this process. In this study, we show that different inducers of ADM increase levels of hydrogen peroxide, most likely generated at the mitochondria, and upregulate the expression of Protein Kinase D1 (PKD1), a kinase that can be activated by hydrogen peroxide. PKD1 expression in acinar cells affects their survival and mediates ADM, which is in part due to the PKD1 target NF-κB. Overall, our data implicate ROS-PKD1 signaling as a common feature of different inducers of pancreatic ADM.
Nutritional Regimes Enriched with Antioxidants as an Efficient Adjuvant for IBD Patients under Infliximab Administration, a Pilot Study
Authors: Liso Marina , Sila Annamaria , Verna Giulio , Scarano Aurelia , Donghia Rossella , Castellana Fabio , Cavalcanti Elisabetta , Pesole Pasqua , Sommella Eduardo , Lippolis Antonio , Armentano Raffaele , Giudetti Anna , Vergara Daniele , Campiglia Pietro , Sardone Rodolfo , Curlo Margherita , Mastronardi Mauro , Petroni Katia , Tonelli Chiara , Santino Angelo , Chieppa Marcello
Published: January 2022

Antioxidants are privileged candidates for the development of adjuvants able to improve the efficiency of pharmacological therapies, particularly for chronic inflammatory syndromes. During the last 20 years, anti-TNFα (tumor necrosis factor alpha) monoclonal antibodies infusion has been the biological therapy most frequently administered but there is still large space for improvement in disease remission rates and maintenance. In this context, nutritional bioactive compounds contained in dietary patterns or included as supplements, may act as adjuvants for the induction and maintenance of IBD (inflammatory bowel diseases) remission. To verify this possibility, a single-center preliminary study (SI-CURA, Soluzioni Innovative per la gestione del paziente e il follow up terapeutico della Colite UlceRosA) was designed and carried out to evaluate whether a daily administration of purple corn supplement could improve the response to Infliximab (IFX) infusion of IBD patients with both Crohn’s disease (CD) and ulcerative colitis (UC). A cohort of 47 patients was enrolled in the study. Biological samples were collected before the first and the third IFX infusion. All patients received nutritional guidelines, 27 of them received commercial red fruit tea with low anthocyanins content, while 20 received a purple corn supplement with a high anthocyanin content. Results show that the administration of an antioxidant-enriched purple corn supplement could improve IFX-mediated disease remission in terms of circulating inflammatory markers. Comparison between CD and UC patients revealed that, at this anthocyanin dosage, the purple corn extract administration improved the IFX response in CD but not in UC patients. Our results may pave the way for a new metacentric study of CD patients, recruiting a wider cohort and followed-up over a longer observational time.
Effect of 5-Azacitidine Treatment on Redox Status and Inflammatory Condition in MDS Patients
Authors: Montes Paola , Guerra-Librero Ana , García Paloma , Cornejo-Calvo María Elena , López María del Señor , Haro Tomás de , Martínez-Ruiz Laura , Escames Germaine , Acuña-Castroviejo Darío
Published: January 2022

This study focused on the impact of the treatment with the hypomethylating agent 5-azacitidine on the redox status and inflammation in 24 MDS patients. Clinical and genetic features of MDS patients were recorded, and peripheral blood samples were used to determine the activity of the endogenous antioxidant defense system (superoxide dismutase, SOD; catalase, CAT; glutathion peroxidase, GPx; and reductase, GRd, activities), markers of oxidative damage (lipid peroxidation, LPO, and advanced oxidation protein products, AOPP). Moreover, pro-inflammatory cytokines and plasma nitrite plus nitrate levels as markers of inflammation, as well as CoQ10 plasma levels, were also measured. Globally, MDS patients showed less redox status in terms of a reduction in the GSSG/GSH ratio and in the LPO levels, as well as increased CAT activity compared with healthy subjects, with no changes in SOD, GPx, and GRd activities, or AOPP levels. When analyzing the evolution from early to advanced stages of the disease, we found that the GPx activity, GSSG/GSH ratio, LPO, and AOPP increased, with a reduction in CAT. GPx changes were related to the presence of risk factors such as high-risk IPSS-R or mutational score. Moreover, there was an increase in IL-2, IL-6, IL-8, and TNF-α plasma levels, with a further increase of IL-2 and IL-10 from early to advanced stages of the disease. However, we did not observe any association between inflammation and oxidative stress. Finally, 5-azacitidine treatment generated oxidative stress in MDS patients, without affecting inflammation levels, suggesting that oxidative status and inflammation are two independent processes.
Rumex japonicus Houtt. Protects Dopaminergic Neurons by Regulating Mitochondrial Function and Gut–Brain Axis in In Vitro and In Vivo Models of Parkinson’s Disease
Authors: Kim Hee-Young , Bae Chang-Hwan , Kim Jayoung , Lee Yukyoung , Jeon Hyongjun , Kim Hyungwoo , Kim Seungtae
Published: January 2022

Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide. Rumex japonicus Houtt. (RJ) has been used to treat gastrointestinal and inflammatory diseases in East Asia. However, it is unknown whether RJ can prevent PD. We investigated the neuroprotective effects of RJ in cellular and animal PD models, focused on mitochondrial function and the gut–brain axis. SH-SY5Y cells were treated with RJ (0.01 mg/mL) for 24 h, after which they were treated with the 1-methyl-4-phenylpyridinium ion (MPP+). MPP+-induced apoptosis increased mitochondrial reactive oxygen species and decreased ATP, PINK1, and DJ-1, which were inhibited by RJ. Ten-week-old C57BL/6N male mice were treated with 30 mg/kg of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) for 5 days and orally administered 50 or 100 mg/kg of RJ for 14 days. RJ alleviated MPTP-induced behavioral impairment, dopaminergic neuronal death, and mitochondrial dysfunction in the substantia nigra (SN) and suppressed the MPTP-induced increase in lipopolysaccharide, interleukin-1β, tumor necrosis factor-α, α-synuclein, and apoptotic factors in the SN and colon. Moreover, RJ inhibited the MPTP-mediated disruption of the tight junction barrier in the colon and blood–brain barrier of mice. Therefore, RJ alleviates MPTP-induced inflammation and dopaminergic neuronal death by maintaining mitochondrial function and tight junctions in the brain and colon.
Overexpression of NOX2 Exacerbates AngII-Mediated Cardiac Dysfunction and Metabolic Remodelling
Authors: Hansen Synne S. , Pedersen Tina M. , Marin Julie , Boardman Neoma T. , Shah Ajay M. , Aasum Ellen , Hafstad Anne D.
Published: January 2022

The present study aimed to examine the effects of low doses of angiotensin II (AngII) on cardiac function, myocardial substrate utilization, energetics, and mitochondrial function in C57Bl/6J mice and in a transgenic mouse model with cardiomyocyte specific upregulation of NOX2 (csNOX2 TG). Mice were treated with saline (sham), 50 or 400 ng/kg/min of AngII (AngII50 and AngII400) for two weeks. In vivo blood pressure and cardiac function were measured using plethysmography and echocardiography, respectively. Ex vivo cardiac function, mechanical efficiency, and myocardial substrate utilization were assessed in isolated perfused working hearts, and mitochondrial function was measured in left ventricular homogenates. AngII50 caused reduced mechanical efficiency despite having no effect on cardiac hypertrophy, function, or substrate utilization. AngII400 slightly increased systemic blood pressure and induced cardiac hypertrophy with no effect on cardiac function, efficiency, or substrate utilization. In csNOX2 TG mice, AngII400 induced cardiac hypertrophy and in vivo cardiac dysfunction. This was associated with a switch towards increased myocardial glucose oxidation and impaired mitochondrial oxygen consumption rates. Low doses of AngII may transiently impair cardiac efficiency, preceding the development of hypertrophy induced at higher doses. NOX2 overexpression exacerbates the AngII -induced pathology, with cardiac dysfunction and myocardial metabolic remodelling.
Identification of Track Stability Model Parameters Based on Numerical Experiments
Authors: Błaszkiewicz-Juszczęć Dorota , Czyczuła Włodzimierz , Kudła Dariusz
Published: January 2022

In the article, an identification method of railway track stability model parameters based on energy equilibrium is presented by the authors. A study of two parameters directly influencing the continuous welded track (CWR) stability is described by the authors, i.e., the rail-sleeper structure stiffness Bz is considered one beam, and the ballast lateral resistance r0. These parameters were estimated with the use of a numerical model for various railway track types. The adopted concept is based on the assumption that it is possible to determine substitute values for both parameters. Therefore, using one value of both of these parameters, we label them substitute parameters. The assumed numerical model forced lateral displacements of a track section, and, based on the obtained track section displacement results, energy equilibrium was determined. The equilibrium takes into account the work of external load and the bending work of rail-sleeper structure with the substitute stiffness Bz and the ballast deformation work, also with the substitute value of lateral resistance r0 with lateral displacement. The aim is to identify these substitute values to be used for analysing track stability with the semi-analytical model. These analyses are part of the studies related to the development of a method of assessing various methods of increasing track stability.
Stochastic Generator of Earthquakes for Mainland France
Authors: Gouache Corentin , Tinard Pierre , Bonneau François
Published: January 2022

Mainland France is characterized by low-to-moderate seismic activity, yet it is known that major earthquakes could strike this territory (e.g., Liguria in 1887 or Basel in 1356). Assessing this French seismic hazard is thus necessary in order to support building codes and to lead prevention actions towards the population. The Probabilistic Seismic Hazard Assessment (PSHA) is the classical approach used to estimate the seismic hazard. One way to apply PSHA is to generate synthetic earthquakes by propagating information from past seismicity and building various seismic scenarios. In this paper, we present an implementation of a stochastic generator of earthquakes and discuss its relevance to mimic the seismicity of low-to-moderate seismic areas. The proposed stochastic generator produces independent events (main shocks) and their correlated seismicity (only aftershocks). Main shocks are simulated first in time and magnitude considering all available data in the area, and then localized in space with the use of a probability map and regionalization. Aftershocks are simulated around main shocks by considering both the seismic moment ratio and distribution of the aftershock’s proportion. The generator is tested with mainland France data.
Laboratory Modeling of an Axial Flow Micro Hydraulic Turbine
Authors: Suslov Daniil , Litvinov Ivan , Gorelikov Evgeny , Shtork Sergey , Wood David
Published: January 2022

This article is devoted to detailed experimental studies of the flow behind the impeller of an air model of a propeller-type microhydroturbine in a wide range of operating parameters. The measurements of two component distributions of averaged velocities and pulsations for conditions from part load to strong overload are conducted. It is shown that the flow at the impeller outlet becomes swirled when the hydraulic turbine operating mode shifts from the optimum one. The character of the behavior of the integral swirl number, which determines the state of the swirled flow, is revealed. Information about the flow peculiarities can be used when adjusting the hydraulic unit mode to optimal conditions and developing recommendations to expand the hydraulic turbine operation control range with preservation of high efficiency. This stage will significantly save time at the stage of equipment design for specific field conditions of water resource.
Analytical Method for the Bending Resistance of Slim Floor Beams with Asymmetric Double-T Steel Section under ISO Fire
Authors: Duma Diana , Zaharia Raul , Pintea Dan , Both Ioan , Hanus Francois
Published: January 2022

The slim floor beams, characterized by the steel profile embedded in the concrete slab, may be found in different configurations, based on the shape of the steel profile cross-section, which can vary from a rectangular to double-T section. While the most common shape used nowadays is the double-T cross-section, the Eurocodes do not provide a simplified method for the fire resistance assessment. The literature offers a simplified method for computation of bending resistance under elevated temperature, based on existing research on thermal models, and was validated for a particular type of slim floor beams (SFB). The current study extends the scope of application of this method, for different types of slim floor beam, which include an asymmetric double-T steel cross-section. The objective was reached through a numerical procedure, by analyzing 162 configurations subjected to four different fire requirements (R30, R60, R90, R120), resulting in a total of 648 analyses, performed with a validated numerical model in SAFIR software. The results in terms of bending resistance showed that the simplified method represents a strong tool for the fire design of slim floor beams.
Monte Carlo Simulation for Exploring Mechanical Properties of Porous Materials Based on Scaled Boundary Finite Element Method
Authors: Liu Guangying , Guo Ran , Zhao Kuiyu , Wang Runjie
Published: January 2022

The existence of pores is a very common feature of nature and of human life, but the existence of pores will alter the mechanical properties of the material. Therefore, it is very important to study the impact of different influencing factors on the mechanical properties of porous materials and to use the law of change in mechanical properties of porous materials for our daily lives. The SBFEM (scaled boundary finite element method) method is used in this paper to calculate a large number of random models of porous materials derived from Matlab code. Multiple influencing factors can be present in these random models. Based on the Monte Carlo simulation, after a large number of model calculations were carried out, the results of the calculations were analyzed statistically in order to determine the variation law of the mechanical properties of porous materials. Moreover, this paper gives fitting formulas for the mechanical properties of different materials. This is very useful for researchers estimating the mechanical properties of porous materials in advance.
Optimal Control for Cleaner Hybrid Vehicles: A Backward Approach
Authors: Jeanneret Bruno , Guille Des Buttes Alice , Keromnes Alan , Pélissier Serge , Le Moyne Luis
Published: January 2022

This work presents an application of the optimal control theory to find trade offs between fuel consumption and pollutant emissions (CO, HC, NOx) of sustaining hybrid vehicles. Both cold start and normal operations are considered. The problem formulation includes two state variables: battery state of energy and catalyst temperature; and three control variables: torque repartition between engine and motor, spark advance, and equivalence ratio. Optimal results were obtained by delaying the first engine crank after the urban part of the mission. The results show that a quick catalyst light off is performed. Once the catalyst is primed, special control parameters values are adopted to operate the engine.