bluuebunny/crossref_metadata_2025 huggingface dataset:
Late Central Airway Toxicity after High-Dose Radiotherapy: Clinical Outcomes and a Proposed Bronchoscopic Classification
Authors: van Hoorn Juliët E. , Dahele Max , Daniels Johannes M. A.
Published: March 2021

The study’s purpose was to identify the bronchoscopic patterns of central airway toxicity following high-dose radiotherapy or chemoradiotherapy, and to look at the consequences of these findings. Our institutional bronchoscopy database was accessed to identify main patterns of airway toxicity observed in a seven-year period. A total of 70 patients were identified with central airway toxicity, and the findings of bronchoscopy were used to derive a classification system. Patient characteristics, time from radiotherapy to toxicity, follow-up and survival were retrospectively analyzed. Results: The main bronchoscopic patterns of airway toxicity were vascular changes (telangiectasia, loss of vascularity, necrosis) and stenosis of the lumen (moderate, severe). Indications for bronchoscopy were airway symptoms (n = 28), assessment post-CRT/surgery (n = 12), (suspected) recurrence (n = 21) or assessment of radiological findings (n = 9). Stenosis was revealed by bronchoscopy at a median time of 10.0 months (IQR: 4–23.5) after radiotherapy and subsequent follow-up after identification was 23 months (IQR: 1.5–55). The corresponding findings for vascular changes were 29 months (IQR: 10.5–48.5), and follow-up after identification was nine months (IQR: 2.5–19.5). There was a statistically significant difference in survival rates between patients with necrosis and telangiectasia (p = 0.002) and loss of vascularity (p = 0.001). Eight out of 10 deceased patients with telangiectasia died of other causes and 4/8 patients with necrosis died of other causes. We identified two main patterns of central airway toxicity visualized with bronchoscopy after high-dose radiotherapy or chemoradiotherapy, and propose a bronchoscopic classification system based on these findings. Preliminary analysis suggests that the pattern and severity of radiation damage might be of prognostic value. Prospective data are required to confirm our findings.
Prognostic and Clinicopathological Significance of CCND1/Cyclin D1 Upregulation in Melanomas: A Systematic Review and Comprehensive Meta-Analysis
Authors: González-Ruiz Lucía , González-Moles Miguel Ángel , González-Ruiz Isabel , Ruiz-Ávila Isabel , Ramos-García Pablo
Published: March 2021

Our objective was to evaluate the prognostic and clinicopathological significance of cyclin D1 (CD1) overexpression/CCND1 amplification in melanomas. We searched studies published before September 2019 (PubMed, Embase, Web of Science, Scopus). We evaluated the quality of the studies included (QUIPS tool). The impact of CD1 overexpression/CCND1 amplification on overall survival and relevant clinicopathological characteristic were meta-analyzed. We performed heterogeneity, sensitivity, small-study effects, and subgroup analyses. Forty-one studies and 3451 patients met inclusion criteria. Qualitative evaluation demonstrated that not all studies were performed with the same rigor, finding the greatest risk of bias in the study confounding domain. Quantitative evaluation showed that immunohistochemical CD1 overexpression had a statistical association with Breslow thickness (p = 0.007; OR = 2.09,95% CI = 1.23–3.57), significantly higher frequency of CCND1/cyclin D1 abnormalities has been observed in the primary tumor compared to distant metastases (p = 0.004), revealed also by immunohistochemical overexpression of the protein (p < 0.001; OR = 0.53,95% CI = 0.40–0.71), while the CCND1 gene amplification does not show association (p = 0.43); while gene amplification, on the contrary, appeared more frequently in distant metastases (p = 0.04; OR = 1.70,95% CI = 1.01–2.85) and not in the primary tumor. In conclusion, CCND1/cyclin D1 upregulation is a common molecular oncogenic alteration in melanomas that probably favors the growth and expansion of the primary tumor. This upregulation is mainly consequence to the overexpression of the cyclin D1 protein, and not to gene amplification.
Longitudinal Changes of Quality of Life and Hearing Following Radiosurgery for Vestibular Schwannoma
Authors: Windisch Paul , Tonn Jörg-Christian , Fürweger Christoph , Ehret Felix , Wowra Berndt , Kufeld Markus , Schichor Christian , Muacevic Alexander
Published: March 2021

Background: Most existing publications on quality of life (QoL) following stereotactic radiosurgery (SRS) for vestibular schwannomas (VS) gather information retrospectively by conducting surveys several years after treatment. The purpose of this study is therefore to provide longitudinal QoL data and assess how changes in hearing impact QoL. Methods: Patients completed the 12-item short-form (SF-12) health survey prior to treatment and at every follow-up visit. One hundred and seventy-five patients who had complete forms prior to treatment as well as at an early and at a late follow-up were included in the analysis. For 51 of these patients, longitudinal audiometry data were available. Results: Median follow-up was 7.2 years. Patients experienced a significant reduction in the physical composite score (PCS, p = 0.011) compared to before treatment. The mental composite score (MCS) increased significantly (p = 0.032). A decrease in PCS was not significantly correlated with an increased hearing threshold on the affected but rather on the unaffected ear (r(49) = −0.32, p = 0.023). Conclusions: It is unclear whether the decline in the PCS is due to treatment-related toxicity or the normal decline of PCS with age. Ensuring proper hearing on the untreated ear might be crucial to ensure good QoL for patients treated with SRS for VS, though this association should be confirmed in additional studies.
Shifting the Gears of Metabolic Plasticity to Drive Cell State Transitions in Cancer
Authors: Wu Zhengwei , Lee Yi Fei , Yeo Xun Hui , Loo Ser Yue , Tam Wai Leong
Published: March 2021

Cancer metabolism is a hallmark of cancer. Metabolic plasticity defines the ability of cancer cells to reprogram a plethora of metabolic pathways to meet unique energetic needs during the various steps of disease progression. Cell state transitions are phenotypic adaptations which confer distinct advantages that help cancer cells overcome progression hurdles, that include tumor initiation, expansive growth, resistance to therapy, metastasis, colonization, and relapse. It is increasingly appreciated that cancer cells need to appropriately reprogram their cellular metabolism in a timely manner to support the changes associated with new phenotypic cell states. We discuss metabolic alterations that may be adopted by cancer cells in relation to the maintenance of cancer stemness, activation of the epithelial–mesenchymal transition program for facilitating metastasis, and the acquisition of drug resistance. While such metabolic plasticity is harnessed by cancer cells for survival, their dependence and addiction towards certain metabolic pathways also present therapeutic opportunities that may be exploited.
Biological Heterogeneity of Chondrosarcoma: From (Epi) Genetics through Stemness and Deregulated Signaling to Immunophenotype
Authors: Zając Agnieszka , Król Sylwia K. , Rutkowski Piotr , Czarnecka Anna M.
Published: March 2021

Chondrosarcoma (ChS) is a primary malignant bone tumor. Due to its heterogeneity in clinical outcomes and resistance to chemo- and radiotherapies, there is a need to develop new potential therapies and molecular targets of drugs. Many genes and pathways are involved in in ChS progression. The most frequently mutated genes are isocitrate dehydrogenase ½ (IDH1/2), collagen type II alpha 1 chain (COL2A1), and TP53. Besides the point mutations in ChS, chromosomal aberrations, such as 12q13 (MDM2) amplification, the loss of 9p21 (CDKN21/p16/INK4A and INK4A-p14ARF), and several gene fusions, commonly occurring in sarcomas, have been found. ChS involves the hypermethylation of histone H3 and the decreased methylation of some transcription factors. In ChS progression, changes in the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K–AKT–mTOR) and hedgehog pathways are known to play a role in tumor growth and chondrocyte proliferation. Due to recent discoveries regarding the potential of immunotherapy in many cancers, in this review we summarize the current state of knowledge concerning cellular markers of ChS and tumor-associated immune cells. This review compares the latest discoveries in ChS biology from gene alterations to specific cellular markers, including advanced molecular pathways and tumor microenvironment, which can help in discovering new potential checkpoints in inhibitory therapy.
Cadmium (II)-Induced Oxidative Stress Results in Replication Stress and Epigenetic Modifications in Root Meristem Cell Nuclei of Vicia faba
Authors: Żabka Aneta , Winnicki Konrad , Polit Justyna Teresa , Wróblewski Mateusz , Maszewski Janusz
Published: March 2021

Among heavy metals, cadmium is considered one of the most toxic and dangerous environmental factors, contributing to stress by disturbing the delicate balance between production and scavenging of reactive oxygen species (ROS). To explore possible relationships and linkages between Cd(II)-induced oxidative stress and the consequent damage at the genomic level (followed by DNA replication stress), root apical meristem (RAM) cells in broad bean (V. faba) seedlings exposed to CdCl2 treatment and to post-cadmium recovery water incubations were tested with respect to H2O2 production, DNA double-strand breaks (γ-phosphorylation of H2AX histones), chromatin morphology, histone H3S10 phosphorylation on serine (a marker of chromatin condensation), mitotic activity, and EdU staining (to quantify cells typical of different stages of nuclear DNA replication). In order to evaluate Cd(II)-mediated epigenetic changes involved in transcription and in the assembly of nucleosomes during the S-phase of the cell cycle, the acetylation of histone H3 on lysine 5 (H3K56Ac) was investigated by immunofluorescence. Cellular responses to cadmium (II) toxicity seem to be composed of a series of interlinked biochemical reactions, which, via generation of ROS and DNA damage-induced replication stress, ultimately activate signal factors engaged in cell cycle control pathways, DNA repair systems, and epigenetic adaptations.
The Pseudo-Circular Genomes of Flaviviruses: Structures, Mechanisms, and Functions of Circularization
Authors: De Falco Louis , Silva Nelly M. , Santos Nuno C. , Huber Roland G. , Martins Ivo C.
Published: March 2021

The circularization of viral genomes fulfills various functions, from evading host defense mechanisms to promoting specific replication and translation patterns supporting viral proliferation. Here, we describe the genomic structures and associated host factors important for flaviviruses genome circularization and summarize their functional roles. Flaviviruses are relatively small, single-stranded, positive-sense RNA viruses with genomes of approximately 11 kb in length. These genomes contain motifs at their 5′ and 3′ ends, as well as in other regions, that are involved in circularization. These motifs are highly conserved throughout the Flavivirus genus and occur both in mature virions and within infected cells. We provide an overview of these sequence motifs and RNA structures involved in circularization, describe their linear and circularized structures, and discuss the proteins that interact with these circular structures and that promote and regulate their formation, aiming to clarify the key features of genome circularization and understand how these affect the flaviviruses life cycle.
Mitochondrial Permeability Transition: A Pore Intertwines Brain Aging and Alzheimer’s Disease
Authors: Jia Kun , Du Heng
Published: March 2021

Advanced age is the greatest risk factor for aging-related brain disorders including Alzheimer’s disease (AD). However, the detailed mechanisms that mechanistically link aging and AD remain elusive. In recent years, a mitochondrial hypothesis of brain aging and AD has been accentuated. Mitochondrial permeability transition pore (mPTP) is a mitochondrial response to intramitochondrial and intracellular stresses. mPTP overactivation has been implicated in mitochondrial dysfunction in aging and AD brains. This review summarizes the up-to-date progress in the study of mPTP in aging and AD and attempts to establish a link between brain aging and AD from a perspective of mPTP-mediated mitochondrial dysfunction.
Chronic Diabetic Wounds and Their Treatment with Skin Substitutes
Authors: Holl Jordan , Kowalewski Cezary , Zimek Zbigniew , Fiedor Piotr , Kaminski Artur , Oldak Tomasz , Moniuszko Marcin , Eljaszewicz Andrzej
Published: March 2021

With the global prevalence of type 2 diabetes mellitus steeply rising, instances of chronic, hard-healing, or non-healing diabetic wounds and ulcers are predicted to increase. The growing understanding of healing and regenerative mechanisms has elucidated critical regulators of this process, including key cellular and humoral components. Despite this, the management and successful treatment of diabetic wounds represents a significant therapeutic challenge. To this end, the development of novel therapies and biological dressings has gained increased interest. Here we review key differences between normal and chronic non-healing diabetic wounds, and elaborate on recent advances in wound healing treatments with a particular focus on biological dressings and their effect on key wound healing pathways.
Catalyst Twenty-Twenty: Post-Traumatic Growth at Scales of Person, Place and Planet
Authors: Logan Alan C. , Berman Susan H. , Scott Richard B. , Berman Brian M. , Prescott Susan L.
Published: March 2021

Planetary health is a broad multidisciplinary effort that attempts to address what has been described as “Anthropocene Syndrome”—the wicked, interrelated challenges of our time. These include, but are not limited to, grotesque biodiversity losses, climate change, environmental degradation, resource depletion, the global burden of non-communicable diseases (NCDs), health inequalities, social injustices, erosion of wisdom and civility, together with the many structural underpinnings of these grand challenges. The ultimate aim of planetary health is flourishing along every link in the person, place and planet continuum. The events of “2020” have illuminated the consequences of “mass trauma” and how sub-threshold anxiety and/or depressive symptoms erase the rigid lines between mental “health” and mental “disorders”, and unmasked the systemic forms of injustice, discrimination, and oppression that have too often escaped discourse. Here, we query the ways in which post-traumatic growth research might inform the larger planetary health community, especially in the context of a global pandemic, broadening socioeconomic inequalities, a worsening climate crisis, and the rise of political authoritarianism. The available research would suggest that “2020” fulfills the trauma criteria of having a “seismic impact on the assumptive world”, and as such, provides fertile ground for post-traumatic growth. Among the many potential positive changes that might occur in response to trauma, we focus on the value of new awareness, perspective and greater wisdom.
Association of Dental Caries and Anthropometric Measures among Primary School Children
Authors: Anzar Wajiha , Qureshi Ambrina , Afaq Ashar , Kattan Hiba F. , Almutairi Basil , Alzahrani Khaled M. , Naseem Mustafa , Vohra Fahim , Abduljabbar Tariq
Published: March 2021

Aim: This study aimed to investigate an association between dental caries status and anthropometric measures in primary school children. Methods and Materials: An analytical cross-sectional study (n = 376) was conducted among primary school children (age range = 6–9 years) registered in private schools. Non-clinical data was gathered from parents of participating children through a self-administered structured questionnaire as well as from the children through an interviewer-administered questionnaire. Clinical data included the examination of dental caries using dmft/DMFT index and anthropometric measures including calculated z-scores of height-for-age (HAZ), weight-for-age (WAZ), BMI-for-age (BAZ), and physical examination. Inferential statistics included Kruskal Wallis and linear regression for univariate and multivariate analysis respectively. Results: The proportion of dental caries in primary and secondary dentition was 67.6% and 8.2% respectively. A significant association was observed between dental caries status and HAZ, WAZ, and BAZ (p < 0.001). An inverse relation was found between low, medium, and high dental caries categories and anthropometric measures. Conclusions: In the primary dentition, dental caries were significantly and inversely related to weight-for-age, height-for-age, and BMI-for-age. Hence, it can be concluded that among the low-income population dental caries is associated with lower anthropometric outcomes in children and therefore caries management should be considered an approach impacting overall health and quality of life.
Implementing a 3As and ‘Opt-Out’ Tobacco Cessation Framework in an Outpatient Oncology Setting
Authors: Himelfarb-Blyth Sarah , Vanderwater Catherine , Hartwick Julia
Published: March 2021

Tobacco cessation has been recognized as an important goal for all ambulatory cancer centres to provide the best possible treatment outcomes and quality of life. However, cessation interventions are applied inconsistently in this setting, with less than one-half of tobacco users being offered evidence-based interventions. The ‘opt-in’ approach traditionally used in cessation, which targets patients who feel ready to quit, may limit the number of patients who are able to receive treatment, and evidence suggests that tobacco users quit at the same rate regardless of their perceived readiness. This paper reports the results of implementing a tobacco cessation framework utilizing the 3As and an ‘opt-out’ approach as a standard of cancer care at a Regional Cancer Centre. A comparison of data from 2017–2018 and 2018–2019 demonstrated an increase in the number of patients screened for tobacco use (76.9% to 90.1%, respectively), and in the number of accepted referrals to quit support (11.5% to 34.7%, respectively). The revised framework was effective at improving referral acceptance rates, despite tobacco use rates remaining stable among the two groups. This demonstrates that employing the ‘opt-out’ approach is a more effective strategy to connect patients with the smoking cessation supports required to optimize their cancer care.
Sustainable Performance Evaluation: Evidence from Listed Chinese Mining Corporations
Authors: Ma Yuan , Men Jingzhi , Li Mingyu , Li Xiaoyan
Published: March 2021

Rapid industrial development has caused a series of environmental problems, which is not conducive to sustainable development of society as a whole. It is necessary to build a sustainable development evaluation system. Most of the existing literature has evaluated corporate sustainable performance from the economy, environment and society on the basis of triple bottom lines. Considering the research gap and the practice need, an evaluation system is established from four dimensions, referred to as economy, society, environment and responsibility management, and 29 indicators are designed to measure these four dimensions. Twenty seven listed Chinese mining corporations are selected as research samples, and the entropy-weight-based Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method is applied to calculate indicators’ weights. Results show that the four dimensions of sustainable performance weights from high to low are society, environment, economy, and management process.
Monitoring Cointegrating Polynomial Regressions: Theory and Application to the Environmental Kuznets Curves for Carbon and Sulfur Dioxide Emissions
Authors: Knorre Fabian , Wagner Martin , Grupe Maximilian
Published: March 2021

This paper develops residual-based monitoring procedures for cointegrating polynomial regressions (CPRs), i.e., regression models including deterministic variables and integrated processes, as well as integer powers, of integrated processes as regressors. The regressors are allowed to be endogenous, and the stationary errors are allowed to be serially correlated. We consider five variants of monitoring statistics and develop the results for three modified least squares estimators for the parameters of the CPRs. The simulations show that using the combination of self-normalization and a moving window leads to the best performance. We use the developed monitoring statistics to assess the structural stability of environmental Kuznets curves (EKCs) for both CO2 and SO2 emissions for twelve industrialized countries since the first oil price shock.
Metal Binding Proteins
Authors: Permyakov Eugene A.
Published: March 2021

Metal ions play several major roles in proteins: structural, regulatory, and enzymatic. The binding of some metal ions increase stability of proteins or protein domains. Some metal ions can regulate various cell processes being first, second, or third messengers. Some metal ions, especially transition metal ions, take part in catalysis in many enzymes. From ten to twelve metals are vitally important for activity of living organisms: sodium, potassium, magnesium, calcium, manganese, iron, cobalt, zinc, nickel, vanadium, molybdenum, and tungsten. This short review is devoted to structural, physical, chemical, and physiological properties of proteins, which specifically bind these metal cations.
Estimation of Surface Canopy Water in Pacific Northwest Forests by Fusing Radar, Lidar, and Meteorological Data
Authors: Heffernan Scott , Strimbu Bogdan M
Published: March 2021

Surface Canopy Water (SCW) is the intercepted rain water that resides within the tree canopy and plays a significant role in the hydrological cycle. Challenges arise in measuring SCW in remote areas using traditional ground-based techniques. Remote sensing in the radio spectrum has the potential to overcome the challenges where traditional modelling approaches face difficulties. In this study, we aim at estimating the SCW by fusing information extracted from the radar imagery acquired with the Sentinel-1 constellation, aerial laser scanning, and meteorological data. To describe the change of radar backscatter with moisture, we focused on six forest stands in the H.J. Andrews experimental forest in central Oregon, as well as four clear cut areas and one golf course, over the summers of 2015–2017. We found significant relationships when we executed the analysis on radar images in which individual tree crowns were delineated from lidar, as opposed to SCW estimated from individual pixel backscatter. Significant differences occur in the mean backscatter between radar images taken during rain vs. dry periods (no rain for >1 h), but these effects only last for roughly 30 min after the end of a rain event. We developed a predictive model for SCW using the radar images acquired at dawn, and proved the capability of space-based radar systems to provide information for estimation of the canopy moisture under conditions of fresh rainfall during the dry season.
A Qualitative Study on the US Forest Service’s Risk Management Assistance Efforts to Improve Wildfire Decision-Making
Authors: Schultz Courtney A. , Miller Lauren F. , Greiner Sarah Michelle , Kooistra Chad
Published: March 2021

To support improved wildfire incident decision-making, in 2017 the US Forest Service (Forest Service) implemented risk-informed tools and processes, together known as Risk Management Assistance (RMA). The Forest Service is developing tools such as RMA to improve wildfire decision-making and implements these tools in complex organizational environments. We assessed the perceived value of RMA and factors that affected its use to inform the literature on decision support for fire management. We sought to answer two questions: (1) What was the perceived value of RMA for line officers who received it?; and (2) What factors affected how RMA was received and used during wildland fire events? We conducted a qualitative study involving semi-structured interviews with decision-makers to understand the contextualized and interrelated factors that affect wildfire decision-making and the uptake of a decision-support intervention such as RMA. We used a thematic coding process to analyze our data according to our questions. RMA increased line officers’ ability to communicate the rationale underlying their decisions more clearly and transparently to their colleagues and partners. Our interviewees generally said that RMA data analytics were valuable but did not lead to changes in their decisions. Line officer personality, pre-season exposure to RMA, local political dynamics and conditions, and decision biases affected the use of RMA. Our findings reveal the complexities of embracing risk management, not only in the context of US federal fire management, but also in other similar emergency management contexts. Attention will need to be paid to existing decision biases, integration of risk management approaches in the interagency context, and the importance of knowledge brokers to connect across internal organizational groups. Our findings contribute to the literature on managing change in public organizations, specifically in emergency decision-making contexts such as fire management.
Joint Offloading and Energy Harvesting Design in Multiple Time Blocks for FDMA Based Wireless Powered MEC
Authors: Yu Zhiyan , Xu Gaochao , Li Yang , Liu Peng , Li Long
Published: March 2021

The combination of mobile edge computing (MEC) and wireless power transfer (WPT) is recognized as a promising technology to solve the problem of limited battery capacities and insufficient computation capabilities of mobile devices. This technology can transfer energy to users by radio frequency (RF) in wireless powered mobile edge computing. The user converts the harvested energy, stores it in the battery, and utilizes the harvested energy to execute corresponding local computing and offloading tasks. This paper adopts the Frequency Division Multiple Access (FDMA) technique to achieve task offloading from multiple mobile devices to the MEC server simultaneously. Our objective is to study multiuser dynamic joint optimization of computation and wireless resource allocation under multiple time blocks to solve the problem of maximizing residual energy. To this end, we formalize it as a nonconvex problem that jointly optimizes the number of offloaded bits, energy harvesting time, and transmission bandwidth. We adopt convex optimization technology, combine with Karush–Kuhn–Tucker (KKT) conditions, and finally transform the problem into a univariate constrained convex optimization problem. Furthermore, to solve the problem, we propose a combined method of Bisection method and sequential unconstrained minimization based on Reformulation-Linearization Technique (RLT). Numerical results demonstrate that the performance of our joint optimization method outperforms other benchmark schemes for the residual energy maximization problem. Besides, the algorithm can maximize the residual energy, reduce the computation complexity, and improve computation efficiency.
Aerodynamic Dispersion of Respiratory Droplets and Aerosols by Turbulent Airflow
Authors: Cornejo Pablo , Guerrero Nicolás , Sandoval Vicente
Published: March 2021

The precautionary measures recommended during the current COVID-19 pandemic do not consider the effect of turbulent airflow. We found the propagation of droplets and aerosols highly affected by this condition. The spread of respiratory droplets by the action of sneezing is characterized by the dynamics of two groups of droplets of different sizes: Larger droplets (300–900 μm) have a ballistic trajectory and can be spread up to 5 m, while a cloud of smaller droplets (100–200 μm) can be transported and dispersed at longer distances up to 18 m by the action of the turbulent airflow. In relation to the spread of exhaled aerosols during respiration, these remain in the air for long periods of time. In the presence of intense or moderate airflow, this set of particles follow airflow streamlines, and thus their propagation is directly determined by the air velocity field. Given the scientific evidence, these results should be considered in public debate about the aerodynamic dispersion characteristics of scenarios where social interactions occur and about the measures to mitigate the spread of the virus.
A Model Based on Clusters of Similar Color and NIR to Estimate Oil Content of Single Olives
Authors: Fredes Claudio , Valero Constantino , Diezma Belén , Mora Marco , Naranjo-Torres José , Wilson Manuel , Delgadillo Gabriel
Published: March 2021

Lipid extraction using the traditional, destructive Soxhlet method is not able to measure oil content (OC) on a single olive. As the color and near infrared spectrum are key parameters to build an oil estimation model (EM), this study grouped olives with similar color and NIR for building EM of oil content obtained by Soxhlet from a cluster of similar olives. The objective was to estimate OC of individual olives, based on clusters of similar color and NIR in two seasons. This study was performed with Arbequina olives in 2016 and 2017. The descriptor of the cluster consisted of the three color channels of c1c2c3 color model plus 11 reflectance points between 1710 and 1735 nm of each olive, normalized with the Z-score index. Clusters of similar color and NIR spectrum were formed with the k-means++ algorithm, leaving a sufficient number of olives to perform the Soxhlet analysis of OC, as reference value of EM. The training of EM was based on Support Vector Machine. The test was performed with Leave One-Out Cross Validation in different training-testing combinations. The best EM predicted the OC with 6 and 13% deviation with respect to the real value when one season was tested with itself and with another season, respectively. The use of clustering in EM is discussed.
Development of a Systematic qPCR Array for Screening GM Soybeans
Authors: Park Saet-Byul , Kim Ji-Yeong , Lee Do-Geun , Kim Jae-Hwan , Shin Min-Ki , Kim Hae-Yeong
Published: March 2021

A screening method using the 35S promoter and nos terminator for genetically modified organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time polymerase chain reaction (also known as quantitative polymerase chain reaction, qPCR) array targeting eight screening assays combined with a prediction system was developed for the rapid tracking of GM soybeans. Each assay’s specificity was tested and confirmed using 17 GM soybean events that have been approved in Korea. The sensitivity of each assay was determined to range from 0.01% to 0.05% using DNA mixtures with different GM ratios, and it was validated by the results of three experimenters. The applicability of this study was tested by monitoring 23 processed foods containing soybeans. It was figured out that 13 of the 23 samples included GM soybeans. The prediction system combined with screening results will be helpful to trace the absence/presence of GM soybean events. This new qPCR array and prediction system for GM soybean detection provides rapid, convenient and reliable results to users.
Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)
Authors: Mo Fulei , Zhang Nian , Qiu Youwen , Meng Lingjun , Cheng Mozhen , Liu Jiayin , Yao Lanning , Lv Rui , Liu Yuxin , Zhang Yao , Chen Xiuling , Wang Aoxue
Published: March 2021

F-box genes play an important role in the growth and development of plants, but there are few studies on its role in a plant’s response to abiotic stresses. In order to further study the functions of F-box genes in tomato (Solanum lycopersicum, Sl), a total of 139 F-box genes were identified in the whole genome of tomato using bioinformatics methods, and the basic information, transcript structure, conserved motif, cis-elements, chromosomal location, gene evolution, phylogenetic relationship, expression patterns and the expression under cold stress, drought stress, jasmonic acid (JA) treatment and salicylic acid (SA) treatment were analyzed. The results showed that SlFBX genes were distributed on 12 chromosomes of tomato and were prone to TD (tandem duplication) at the ends of chromosomes. WGD (whole genome duplication), TD, PD (proximal duplication) and TRD (transposed duplication) modes seem play an important role in the expansion and evolution of tomato SlFBX genes. The most recent divergence occurred 1.3042 million years ago, between SlFBX89 and SlFBX103. The cis-elements in SlFBX genes’ promoter regions were mainly responded to phytohormone and abiotic stress. Expression analysis based on transcriptome data and qRT-PCR (Real-time quantitative PCR) analysis of SlFBX genes showed that most SlFBX genes were differentially expressed under abiotic stress. SlFBX24 was significantly up-regulated at 12 h under cold stress. This study reported the SlFBX gene family of tomato for the first time, providing a theoretical basis for the detailed study of SlFBX genes in the future, especially the function of SlFBX genes under abiotic stress.
Peripheral Blood MicroRNAs as Potential Biomarkers of Myocardial Damage in Acute Viral Myocarditis
Authors: Marketou Maria , Kontaraki Joanna , Patrianakos Alexandros , Kochiadakis George , Anastasiou Ioannis , Fragkiadakis Konstantinos , Plevritaki Anthoula , Papadaki Sofia Thalia , Chlouverakis Gregory , Parthenakis Fragiskos
Published: March 2021

Background: microRNAs (miRs) have emerged as important modulators of cardiovascular development and disease. Our aim was to determine whether cardiac-related miRs such as miR-21-5p and miR-1-3p were differentially expressed in acute viral myocarditis and whether any of them was related with the extent of myocardial damage and left ventricular dysfunction. Methods: We enrolled 40 patients with acute viral myocarditis. Blood samples were taken on admission and miRs expression levels in peripheral blood mononuclear cells were quantified by real-time reverse transcription polymerase chain reaction. Results: miR-21-5p, miR-1-3p were significantly elevated in acute myocarditis. miR-21-5p levels showed a strong correlation with global longitudinal strain (r = 0.71, p < 0.01), while miR-1-3p had significant correlations with troponin I (r = 0.79, p < 0.01). Conclusions: The expression of miR-21-5p and miR-1-3p in peripheral blood is increased in acute viral myocarditis, and this increase is correlated with myocardial damage and indicative of left ventricular systolic dysfunction in these patients.
Molecular Pathways and Pigments Underlying the Colors of the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus 1758)
Authors: Stenger Pierre-Louis , Ky Chin-Long , Reisser Céline , Duboisset Julien , Dicko Hamadou , Durand Patrick , Quintric Laure , Planes Serge , Vidal-Dupiol Jeremie
Published: March 2021

The shell color of the Mollusca has attracted naturalists and collectors for hundreds of years, while the molecular pathways regulating pigment production and the pigments themselves remain poorly described. In this study, our aim was to identify the main pigments and their molecular pathways in the pearl oyster Pinctada margaritifera—the species displaying the broadest range of colors. Three inner shell colors were investigated—red, yellow, and green. To maximize phenotypic homogeneity, a controlled population approach combined with common garden conditioning was used. Comparative analysis of transcriptomes (RNA-seq) of P. margaritifera with different shell colors revealed the central role of the heme pathway, which is involved in the production of red (uroporphyrin and derivates), yellow (bilirubin), and green (biliverdin and cobalamin forms) pigments. In addition, the Raper–Mason, and purine metabolism pathways were shown to produce yellow pigments (pheomelanin and xanthine) and the black pigment eumelanin. The presence of these pigments in pigmented shell was validated by Raman spectroscopy. This method also highlighted that all the identified pathways and pigments are expressed ubiquitously and that the dominant color of the shell is due to the preferential expression of one pathway compared with another. These pathways could likely be extrapolated to many other organisms presenting broad chromatic variation.
Genetic Drivers of Head and Neck Squamous Cell Carcinoma: Aberrant Splicing Events, Mutational Burden, HPV Infection and Future Targets
Authors: Dlamini Zodwa , Alaouna Mohammed , Mbatha Sikhumbuzo , Bhayat Ahmed , Mabongo Mzubanzi , Chatziioannou Aristotelis , Hull Rodney
Published: March 2021

Head and neck cancers include cancers that originate from a variety of locations. These include the mouth, nasal cavity, throat, sinuses, and salivary glands. These cancers are the sixth most diagnosed cancers worldwide. Due to the tissues they arise from, they are collectively named head and neck squamous cell carcinomas (HNSCC). The most important risk factors for head and neck cancers are infection with human papillomavirus (HPV), tobacco use and alcohol consumption. The genetic basis behind the development and progression of HNSCC includes aberrant non-coding RNA levels. However, one of the most important differences between healthy tissue and HNSCC tissue is changes in the alternative splicing of genes that play a vital role in processes that can be described as the hallmarks of cancer. These changes in the expression profile of alternately spliced mRNA give rise to various protein isoforms. These protein isoforms, alternate methylation of proteins, and changes in the transcription of non-coding RNAs (ncRNA) can be used as diagnostic or prognostic markers and as targets for the development of new therapeutic agents. This review aims to describe changes in alternative splicing and ncRNA patterns that contribute to the development and progression of HNSCC. It will also review the use of the changes in gene expression as biomarkers or as the basis for the development of new therapies.
“It Makes You Feel That You Are There”: Exploring the Acceptability of Virtual Reality Nature Environments for People with Memory Loss
Authors: Orr Noreen , Yeo Nicola L. , Dean Sarah G. , White Mathew P. , Garside Ruth
Published: March 2021

Aim: To report on the acceptability of virtual reality (VR) nature environments for people with memory loss at memory cafes, and explore the experiences and perceptions of carers and staff. Methods: A qualitative study was conducted between January and March 2019. Ten adults with memory loss, eight carers and six volunteer staff were recruited from two memory cafes, located in Cornwall, UK. There were 19 VR sessions which were audio recorded and all participants were interviewed at the end of the sessions. Framework analysis was used to identify patterns and themes in the data. Results: During the VR experience, participants were engaged to varying degrees, with engagement facilitated by the researcher, and in some cases, with the help of a carer. Participants responded positively to the nature scenes, finding them soothing and evoking memories. The VR experience was positive; many felt immersed in nature and saw it as an opportunity to ‘go somewhere’. However, it was not always positive and for a few, it could be ‘strange’. Participants reflected on their experience of the VR equipment, and volunteer staff and carers also shared their perceptions of VR for people with dementia in long-term care settings. Conclusions: The VR nature experience was an opportunity for people with memory loss to be immersed in nature and offered the potential to enhance their quality of life. Future work should build on lessons learned and continue to work with people with dementia in developing and implementing VR technology in long-term care settings.
Drug-Related Problems in Prescribing for Pediatric Outpatients in Vietnam
Authors: Nguyen Thao H. , Le Vy T. T. , Quach Dung N. , Diep Han G. , Nguyen Nguyet K. , Lam Anh N. , Pham Suol T. , Taxis Katja , Nguyen Thang , Nguyen Phuong M.
Published: March 2021

Background: Our study was conducted to determine the prevalence of drug-related problems (DRPs) in outpatient prescriptions, the impact of DRPs on treatment efficacy, safety, and cost, and the determinants of DRPs in prescribing for pediatric outpatients in Vietnam. Methods: A retrospective cross-sectional study was conducted on pediatric outpatients at a pediatric hospital in Can Tho, Vietnam. DRPs were classified according to the Pharmaceutical Care Network Europe classification (PCNE) of 2020. The study determined prevalence of DRPs and their impacts on efficacy, safety, and cost. Multivariate regression was used to identify the determinants of DRPs. Results: The study included 4339 patients (mean age 4.3, 55.8% male), with a total of 3994 DRPs, averaging 0.92 DRP/prescription. The proportion of prescriptions with at least one DRP was 65.7%. DRPs included inappropriate drug selection (35.6%), wrong time of dosing relative to meals (35.6%), inappropriate dosage form (9.3%), inappropriate indication (7.1%), and drug-drug interactions (0.3%). The consensus of experts was average when evaluating each aspect of efficiency reduction, safety reduction, and treatment cost increase, with Fleiss’ coefficients of 0.558, 0.511, and 0.541, respectively (p < 0.001). Regarding prescriptions, 50.1% were assessed as reducing safety. The figures for increased costs and decreased treatment effectiveness were 29.0% and 23.9%, respectively. Patients who were ≤2 years old were more likely to have DRPs than patients aged 2 to 6 years old (OR = 0.696; 95% CI = 0.599–0.809) and patients aged over 6 years old (OR = 0.801; 95% CI = 0.672–0.955). Patients who had respiratory system disease were more likely to have DRPs than patients suffering from other diseases (OR = 0.715; 95% CI = 0.607–0.843). Patients with comorbidities were less likely to have DRPs than patients with no comorbidities (OR = 1.421; 95% CI = 1.219–1.655). Patients prescribed ≥5 drugs were more likely to have DRPs than patients who took fewer drugs (OR = 3.677; 95% CI = 2.907–4.650). Conclusion: The proportion of prescriptions in at least one DRP was quite high. Further studies should evaluate clinical significance and appropriate interventions, such as providing drug information and consulting doctors about DRPs.
Overview of the Dynamic Role of Specialty Cut Flowers in the International Cut Flower Market
Authors: Darras Anastasios
Published: March 2021

The global cut flower industry has faced serious challenges over the years, but still remains an important sector of agriculture. Floriculture businesses seek new, innovative trends and niches to help increase product sales. Specialty cut flower (SCF) production has increased in the past 20 years in the US, Australia, Africa, and Europe. SCF production and sales could increase further if these new products were supported by dynamic marketing campaigns that focus on their strengths compared to the traditional cut flowers (TCF) such as roses, carnations, gerberas, and chrysanthemums. The major strength of SCF is the eco-friendly profile, which is associated to low CO2 footprints and environmental outputs. This contrasts TCF cultivation, which is associated to high energy inputs, especially at the traditional production centres (e.g., The Netherlands). It is suggested that environmental legislations, production costs, and customer demand for eco-friendly products will positively affect future SCF cultivation and sale.
Factors Influencing the Dementia-Preventive Behaviors among Middle-Aged Persons with Chronic Diseases in Korea
Authors: Park Jisung , Shin Juh Hyun
Published: March 2021

The purpose of this study was to investigate the influence of dementia literacy, internal health locus of control, and fear of dementia on dementia-preventive behaviors among middle-aged people with chronic diseases. The participants were middle-aged individuals with chronic diseases who had been taking medications for at least three months, recruited using convenience sampling. A total of 123 participants were recruited between 13 and 31 March 2020, using self-reported questionnaires. Data were then analyzed through independent t-test, one-way ANOVA, Pearson’s correlation coefficient, and multiple linear regression using the SPSS/WIN 25.0. The results showed that health condition perceived as healthy and dementia literacy were the leading factors influencing dementia-preventive behaviors. These variables showed a 16% explanatory power for dementia-preventive behaviors. Therefore, when the participants’ perceived health condition was healthy and the dementia literacy score was higher, the level of dementia-preventive behaviors was also higher. It is necessary to develop educational programs to increase dementia literacy as a major variable in dementia-preventive behaviors, and further research on its efficacy should be conducted. When providing dementia-preventive education programs to middle-aged people who have been exposed to chronic diseases, it is necessary to consider their level of perceived health condition and dementia literacy.
The Validation of Chinese Version of the Victimization Subscale of the Revised Peer Experiences Questionnaire
Authors: Fan Liheng , Liu Bu , Jin Zheng , Zhu Xiangru
Published: March 2021

In China, researchers have translated and validated several scales to measure victimization behavior. The aim of the present study was to validate the Chinese version of the victimization subscale of the Revised Peer Experiences Questionnaire (RPEQ) among primary school students. Primary school students aged between 8 and 13 years old (n = 1048) were asked to complete the Chinese version of the victimization subscale of the RPEQ and related scales. We examined internal consistency and the factor structure using confirmatory factor analysis (CFA). Depression, peer relationship, and sleep scales were used to measure construct validity. The CFA results suggested that the four-factor model had a good model fit. The results indicated that internal reliability was good (Cronbach’s α = 0.83). Construct validity was mostly supported by scores on the Chinese version of the victimization subscale of the RPEQ that strongly and positively correlated with depression and negatively correlated with peer relationship and sleep quality. The present study indicated that the Chinese version of the victimization subscale of the RPEQ has adequate reliability and validity for measuring bullying problems among Chinese primary school students.
Relation among Perceived Weight Change, Sedentary Activities and Sleep Quality during COVID-19 Lockdown: A Study in an Academic Community in Northern Italy
Authors: Micheletti Cremasco Margherita , Mulasso Anna , Moroni Alessia , Testa Andrea , Degan Raffaella , Rainoldi Alberto , Rabaglietti Emanuela
Published: March 2021

In Italy, COVID-19 lockdown was imposed from 8 March until 3 May 2020 with negative consequences on the lifestyles and health of people. Within this context, the paper aims: (i) to analyse the impact of COVID-19 lockdown on perceived weight changes; (ii) to evaluate factors associated with the perception of weight changes (Body Mass Index (BMI), sleep quality, time spent in sedentary activities), in an Italian academic community of students and workers. A total of 3666 participants took part in this cross-sectional study (2838 students and 828 workers, of whom 73.0% were female). T-test, Chi-square test and the two-way ANOVA were used. Results showed that 43.3% of participants perceived a weight gain. Workers experienced a more substantial increase in body weight (0.7 kg) compared to students (0.3 kg; p = 0.013). A significant difference between preobese/obese workers (0.9 kg) and students (−0.3 kg; p < 0.001) was found. Overall, 57.0% of the sample was characterized by high levels of sedentary activities. Sedentary people noticed a higher weight gain (0.4 kg) compared to less sedentary people (0.3 kg; p = 0.048). More than 45% of participants reported a worsening of sleep quality and showed a perceived increase in body weight (0.5 kg) in comparison to those who improved their sleep quality (no weight change; p = 0.001). Designing tailored interventions to promote health-related behaviours during lockdown periods is essential.