bluuebunny/crossref_metadata_2025 huggingface dataset:
Acid–Base Balance, Blood Gases Saturation, and Technical Tactical Skills in Kickboxing Bouts According to K1 Rules
Authors: Rydzik Łukasz , Mardyła Mateusz , Obmiński Zbigniew , Więcek Magdalena , Maciejczyk Marcin , Czarny Wojciech , Jaszczur-Nowicki Jarosław , Ambroży Tadeusz
Published: January 2022

Background: Acid–base balance (ABB) is a major component of homeostasis, which is determined by the efficient functioning of many organs, including the lungs, kidneys, and liver, and the proper water and electrolyte exchange between these components. The efforts made during competitions by combat sports athletes such as kickboxers require a very good anaerobic capacity, which, as research has shown, can be improved by administering sodium bicarbonate. Combat sports are also characterized by an open task structure, which means that cognitive and executive functions must be maintained at an appropriate level during a fight. The aim of our study was to analyze the changes in ABB in capillary blood, measuring levels of H+, pCO2, pO2, HCO3−, BE and total molar CO2 concentration (TCO2), which were recorded 3 and 20 min after a three-round kickboxing bout, and the level of technical and tactical skills presented during the fight. Methods: The study involved 14 kickboxers with the highest skill level (champion level). Statistical comparison of mentioned variables recorded prior to and after a bout was done with the use of Friedman’s ANOVA. Results: 3 min after a bout, H+ and pO2 were higher by 41% and 11.9%, respectively, while pCO2, HCO3−, BE and TO2 were lower by 14.5%, 39.4%, 45.4% and 34.4%, respectively. Furthermore, 20 min after the bout all variables tended to normalization and they did not differ significantly compared to the baseline values. Scores in activeness of the attack significantly correlated (r = 0.64) with pre–post changes in TCO2. Conclusions: The disturbances in ABB and changes in blood oxygen and carbon dioxide saturation observed immediately after a bout indicate that anaerobic metabolism plays a large part in kickboxing fights. Anaerobic training should be included in strength and conditioning programs for kickboxers to prepare the athletes for the physiological requirements of sports combat.
High-Intensity Interval Training Improves Cardiac Autonomic Function in Patients with Type 2 Diabetes: A Randomized Controlled Trial
Authors: Silva Lucas Raphael Bento , Gentil Paulo , Seguro Camila Simões , de Oliveira Jordana Campos Martins , Silva Maria Sebastiana , Marques Vitor Alves , Beltrame Thomas , Rebelo Ana Cristina Silva
Published: January 2022

Different exercise models have been used in patients with type 2 diabetes mellitus (T2D), like moderate intensity continuous training (MICT) and high intensity interval training (HIIT); however, their effects on autonomic modulation are unknown. The present study aimed to compare the effects of different exercise modes on autonomic modulation in patients with T2D. In total, 44 adults with >5 years of T2D diagnosis were recruited and stratified into three groups: HIIT-30:30 (n = 15, age 59.13 ± 5.57 years) that performed 20 repetitions of 30 s at 100% of VO2peak with passive recovery, HIIT-2:2 (n = 14, age 61.20 ± 2.88) that performed 5 repetitions of 2 min at 100% of VO2peak with passive recovery, and MICT (n = 15, age 58.50 ± 5.26) that performed 14 min of continuous exercise at 70% of VO2peak. All participants underwent anamnesis and evaluation of cardiorespiratory fitness and cardiac autonomic modulation. All protocols were equated by total distance and were performed two times per week for 8 weeks. Group × time interactions were observed for resting heart rate (HRrest) [F(2.82) = 3.641; p = 0.031] and SDNN [F(2.82) = 3.462; p = 0.036]. Only the HIIT-30:30 group significantly reduced SDNN (p = 0.002 and 0.025, respectively). HRrest reduced more in the HIIT-30:30 group compared with the MICT group (p = 0.038). Group × time interactions were also observed for offTAU [F(2.82) = 3.146; p = 0.048] and offTMR [F(2.82) = 4.424; p = 0.015]. The MICT group presented increased values of offTAU compared with the HIIT-30:30 and HIIT-2:2 groups (p = 0.001 and 0.013, respectively), representing a slower HR response after eight weeks of intervention. HIIT, specially HIIT-30:30, represents a promising measure for improving autonomic modulation in patients with T2D.
Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
Authors: Yi Xingwan , Gao Huabei , Yang Yi , Yang Shumin , Luo Le , Yu Chao , Wang Jia , Cheng Tangren , Zhang Qixiang , Pan Huitang
Published: January 2022

Roses are the most important cut flower crops and widely used woody ornamental plants in gardens throughout the world, and they are model plants for studying the continuous-flowering trait of woody plants. To analyze the molecular regulation mechanism of continuous flowering, comparative transcriptome data of once- and continuous-flowering roses in our previous study were used to conduct weighted gene co-expression network analysis (WGCNA) to obtain the candidate genes related to flowering transitions. The expression patterns of candidate genes at different developmental stages between Rosa chinensis “Old Blush” (continuous-flowering cultivar) and R. “Huan Die” (once-flowering cultivar) were investigated, and the relationship of the key gene with the endogenous hormone was analyzed. The results showed that the expression trends of VIN3-LIKE 1 (VIL1), FRIGIDA- LIKE 3 (FRI3), APETALA 2- LIKE (AP2-like) and CONSTANS-LIKE 2 (CO-like 2) genes were significantly different between “Old Blush” and “Huan Die”, and the expression trends of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) and CO-like 2 were consistent in the flowering transition of “Old Blush” under different environments. The changes in cytokinin and gibberellic acid (GA3) content were different in the two rose cultivars. The overall change trend of the abscisic acid and GA3 in the flowering transition of “Old Blush” under different environments was consistent. The promoter sequence of CO-like 2 contained a P-box element associated with gibberellin response, as well as binding sites for transcription factors. In a word, we found CO-like 2 associated with continuous flowering and some factors that may synergistically regulate continuous flowering. The results provided a reference for elucidating the molecular regulatory mechanisms of continuous-flowering traits in roses.
HMGB-1 in Psoriasis
Authors: Casciaro Marco , Di Salvo Eleonora , Gangemi Sebastiano
Published: January 2022

Psoriasis is a multifactorial pathology linked to systemic inflammation. Enhanced keratinocytes proliferation and a minor maturation state of the cells are typical features. Perivascular T cells, dendritic cells, macrophages, and neutrophilic granulocytes are part of the scenario completed by apoptosis dysregulation. Several proinflammatory mediators, alarmins and growth factors are increased too, both in the skin and the patients’ blood. HMGB1 is important as an alarmin in several inflammatory conditions. Released after cellular damage, HMGB1 acts as a danger signal. Several studies have considered its role in psoriasis pathogenesis. We evaluated its level in psoriasis and the potential of the alarmin blockade through standard therapies, biological treatments and using monoclonal antibodies. PV patients were shown to have significantly increased levels of HMGB1 both in lesional skin and in serum, which were linked, in some cases, to other pro-inflammatory markers and alarmins. In most cases these parameters were correlated with PASI score. Data demonstrated that blocking HMGB1 is effective in ameliorating psoriasis. Focusing on this approach could be valuable in terms of a therapeutic option for counteracting immune-related diseases in a way unthinkable until few years ago.
Rational Development and Characterization of a Ubiquitin Variant with Selectivity for Ubiquitin C-Terminal Hydrolase L3
Authors: Hewitt Chad S. , Das Chittaranjan , Flaherty Daniel P.
Published: January 2022

There is currently a lack of reliable methods and strategies to probe the deubiquitinating enzyme UCHL3. Current small molecules reported for this purpose display reduced potency and selectivity in cellular assays. To bridge this gap and provide an alternative approach to probe UCHL3, our group has carried out the rational design of ubiquitin-variant activity-based probes with selectivity for UCHL3 over the closely related UCHL1 and other DUBs. The approach successfully produced a triple-mutant ubiquitin variant activity-based probe, UbVQ40V/T66K/V70F-PRG, that was ultimately 20,000-fold more selective for UCHL3 over UCHL1 when assessed by rate of inactivation assays. This same variant was shown to selectively form covalent adducts with UCHL3 in MDA-MB-231 breast cancer cells and no reactivity toward other DUBs expressed. Overall, this study demonstrates the feasibility of the approach and also provides insight into how this approach may be applied to other DUB targets.
Bifidobacterium bifidum Shows More Diversified Ways of Relieving Non-Alcoholic Fatty Liver Compared with Bifidobacterium adolescentis
Authors: Wang Linlin , Jiao Ting , Yu Qiangqing , Wang Jialiang , Wang Luyao , Wang Gang , Zhang Hao , Zhao Jianxin , Chen Wei
Published: January 2022

The occurrence of non-alcoholic fatty liver disease (NAFLD) is closely related to intestinal microbiota disturbance, and probiotics has become a new strategy to assist in alleviating NAFLD. In order to investigate the effect of Bifidobacterium on NAFLD and the possible pathway, a NAFLD model was established by using a high-fat diet (HFD) for 18 weeks. Fourteen strains of Bifidobacterium were selected (seven Bifidobacterium adolescentis and seven Bifidobacterium bifidum) for intervention. The effects of different bifidobacteria on NAFLD were evaluated from liver cell injury, liver fat deposition, liver inflammatory state and liver histopathology, and were taken as entry points to explore the mitigation approaches of bifidobacteria through energy intake, lipid metabolism, glucose metabolism and intestinal permeability. The results showed that Bifidobacterium exerts species-specific effects on NAFLD. B. bifidum exerted these effects mainly through regulating the intestinal microbiota, increasing the relative abundance of Faecalibaculum and Lactobacillus, decreasing the relative abundance of Tyzzerella, Escherichia-Shigella, Intestinimonas, Osillibacter and Ruminiclostridium, and further increasing the contents of propionic acid and butyric acid, regulating lipid metabolism and intestinal permeability, and ultimately inhibiting liver inflammation and fat accumulation to alleviate NAFLD. B. adolescentis exerted its effects mainly through changing the intestinal microbiota, increasing the content of propionic acid, regulating lipid metabolism and ultimately inhibiting liver inflammation to alleviate NAFLD.
Endogenous Levels of Gamma Amino-Butyric Acid Are Correlated to Glutamic-Acid Decarboxylase Antibody Levels in Type 1 Diabetes
Authors: Hill Henrik , Elksnis Andris , Lundkvist Per , Ubhayasekera Kumari , Bergquist Jonas , Birnir Bryndis , Carlsson Per-Ola , Espes Daniel
Published: January 2022

Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system (CNS) and outside of the CNS, found in the highest concentrations in immune cells and pancreatic beta-cells. GABA is gaining increasing interest in diabetes research due to its immune-modulatory and beta-cell stimulatory effects and is a highly interesting drug candidate for the treatment of type 1 diabetes (T1D). GABA is synthesized from glutamate by glutamic acid decarboxylase (GAD), one of the targets for autoantibodies linked to T1D. Using mass spectrometry, we have quantified the endogenous circulating levels of GABA in patients with new-onset and long-standing T1D and found that the levels are unaltered when compared to healthy controls, i.e., T1D patients do not have a deficit of systemic GABA levels. In T1D, GABA levels were negatively correlated with IL-1 beta, IL-12, and IL-15 15 and positively correlated to levels of IL-36 beta and IL-37. Interestingly, GABA levels were also correlated to the levels of GAD-autoantibodies. The unaltered levels of GABA in T1D patients suggest that the GABA secretion from beta-cells only has a minor impact on the circulating systemic levels. However, the local levels of GABA could be altered within pancreatic islets in the presence of GAD-autoantibodies.
The Organization of Somatostatin-Immunoreactive Cells in the Visual Cortex of the Gerbil
Authors: Kwon Kyung-Min , Lee Myung-Jun , Chung Han-Saem , Pak Jae-Hong , Jeon Chang-Jin
Published: January 2022

Somatostatin (SST) is widely expressed in the brain and plays various, vital roles involved in neuromodulation. The purpose of this study is to characterize the organization of SST neurons in the Mongolian gerbil visual cortex (VC) using immunocytochemistry, quantitative analysis, and confocal microscopy. As a diurnal animal, the Mongolian gerbil provides us with a different perspective to other commonly used nocturnal rodent models. In this study, SST neurons were located in all layers of the VC except in layer I; they were most common in layer V. Most SST neurons were multipolar round/oval or stellate cells. No pyramidal neurons were found. Moreover, 2-color immunofluorescence revealed that only 33.50%, 24.05%, 16.73%, 0%, and 64.57% of SST neurons contained gamma-aminobutyric acid, calbindin-D28K, calretinin, parvalbumin, and calcium/calmodulin-dependent protein kinase II, respectively. In contrast, neuropeptide Y and nitric oxide synthase were abundantly expressed, with 80.07% and 75.41% in SST neurons, respectively. Our immunocytochemical analyses of SST with D1 and D2 dopamine receptors and choline acetyltransferase, α7 and β2 nicotinic acetylcholine receptors suggest that dopaminergic and cholinergic fibers contact some SST neurons. The results showed some distinguishable features of SST neurons and provided some insight into their afferent circuitry in the gerbil VC. These findings may support future studies investigating the role of SST neurons in visual processing.
A Comparison of Various Chips Used for the Manufacture of Biosensors Applied in Non-Fluidic Array SPRi, Based on the Example of Determination of Cathepsin D
Authors: Falkowski Pawel , Mrozek Piotr , Miluski Piotr , Lukaszewski Zenon , Gorodkiewicz Ewa
Published: January 2022

Non-fluidic array SPR imaging (SPRi) with appropriate biosensors is a new tool for the determination of various biomarkers in body fluids. Numerous biomarkers can be determined without signal enhancement or preliminarily preconcentration. The introduction of a new material solution of the chip may increase the scope of the application of this technique. Solutions with adhesive separating foil and an Ag/Au chip were compared with the previously used two-paint separating polymer and pure gold chip. These solutions were tested using the example of a biosensor for cathepsin D (Cath D), which consisted of pepstatin A (a Cath D inhibitor) immobilized via a cysteamine linker using the NHS/EDC protocol. Four material versions of the Cath D biosensor proved adequate in terms of range of linearity, LOQ, precision and recovery. All four versions of the biosensor were used for the determination of Cath D in the blood serum patients with glioblastoma and control samples, producing very similar results and showing an elevated biomarker concentration in the case of cancer. Therefore, the problem of determining the correct level of Cath D in the serum of healthy individuals has been resolved, correcting literature data which ranged over three orders of magnitude.
Cerebral Microstructure Analysis by Diffusion-Based MRI in Systemic Lupus Erythematosus: Lessons Learned and Research Directions
Authors: Silvagni Ettore , Bortoluzzi Alessandra , Borrelli Massimo , Bianchi Andrea , Fainardi Enrico , Govoni Marcello
Published: January 2022

Diffusion-based magnetic resonance imaging (MRI) studies, namely diffusion-weighted imaging (DWI) and diffusion-tensor imaging (DTI), have been performed in the context of systemic lupus erythematosus (SLE), either with or without neuropsychiatric (NP) involvement, to deepen cerebral microstructure alterations. These techniques permit the measurement of the variations in random movement of water molecules in tissues, enabling their microarchitecture analysis. While DWI is recommended as part of the initial MRI assessment of SLE patients suspected for NP involvement, DTI is not routinely part of the instrumental evaluation for clinical purposes, and it has been mainly used for research. DWI and DTI studies revealed less restricted movement of water molecules inside cerebral white matter (WM), expression of a global loss of WM density, occurring in the context of SLE, prevalently, but not exclusively, in case of NP involvement. More advanced studies have combined DTI with other quantitative MRI techniques, to further characterize disease pathogenesis, while brain connectomes analysis revealed structural WM network disruption. In this narrative review, the authors provide a summary of the evidence regarding cerebral microstructure analysis by DWI and DTI studies in SLE, focusing on lessons learned and future research perspectives.
The Quest for Psychiatric Advancement through Theory, beyond Serendipity
Authors: Kelly Robert E. , Ahmed Anthony O. , Hoptman Matthew J. , Alix Anika F. , Alexopoulos George S.
Published: January 2022

Over the past century, advancements in psychiatric treatments have freed countless individuals from the burden of life-long, incapacitating mental illness. These treatments have largely been discovered by chance. Theory has driven advancement in the natural sciences and other branches of medicine, but psychiatry remains a field in its “infancy”. The targets for healing in psychiatry lie within the realm of the mind’s subjective experience and thought, which we cannot yet describe in terms of their biological underpinnings in the brain. Our technology is sufficiently advanced to study brain neurons and their interactions on an electrophysiological and molecular level, but we cannot say how these form a single feeling or thought. While psychiatry waits for its “Copernican Revolution”, we continue the work in developing theories and associated experiments based on our existing diagnostic systems, for example, the Diagnostic and Statistical Manual of Mental Disorders (DSM), International Classification of Diseases (ICD), or the more newly introduced Research Domain Criteria (RDoC) framework. Understanding the subjective reality of the mind in biological terms would doubtless lead to huge advances in psychiatry, as well as to ethical dilemmas, from which we are spared for the time being.
Neurological Manifestations in a Cohort of Egyptian Patients with COVID-19: A Prospective, Multicenter, Observational Study
Authors: Mekkawy Doaa A. , Hamdy Sherif , Abdel-Naseer Maged , Shehata Hatem S. , Halfawy Ahmed Al , Shalaby Nevin M. , Shehata Ghaydaa A. , Ali Anwar M. , Elmazny Alaa , Ahmed Sandra M. , Ismail Jumana H. , Ibraheim Aml , Abdel-Hamid Hoda M. , Magdy Rehab , Kabara Ayoub Younan , Taha Ahmed E. , Merghany Nahla , Soliman Nourhan M. , Elshebawy Haidy , Abdelal Samar E. S. , El-Ghoneimy Lobna , Al-Athwari Aussan , Kishk Nirmeen A. , Nada Mona A. F. , Farghaly Marwa , Hassan Amr , Hegazy Mohamed I. , Abdelalim Ahmed , Mourad Husam S. , Hassouna Amira , Othman Alshimaa S. , Wijeratne Tissa
Published: January 2022

Background: The COVID-19 pandemic has reached over 276 million people globally with 5.3 million deaths as of 22nd December 2021. COVID-19-associated acute and long-term neurological manifestations are well recognized. The exact profile and the timing of neurological events in relation to the onset of infection are worth exploring. The aim of the current body of work was to determine the frequency, pattern, and temporal profile of neurological manifestations in a cohort of Egyptian patients with confirmed COVID-19 infection. Methods: This was a prospective study conducted on 582 hospitalized COVID-19 patients within the first two weeks of the diagnosis of COVID-19 to detect any specific or non-specific neurological events. Results: The patients’ mean (SD) age was 46.74 (17.26) years, and 340 (58.42%) patients were females. The most commonly encountered COVID-19 symptoms were fever (90.72%), cough (82.99%), and fatigue (76.98%). Neurological events (NE) detected in 283 patients (48.63%) and were significantly associated with a severe COVID-19 at the onset (OR: 3.13; 95% CI: 2.18–4.51; p < 0.0001) and with a higher mortality (OR: 2.56; 95% CI: 1.48–5.46; p = 0.019). The most frequently reported NEs were headaches (n = 167) and myalgias (n = 126). Neurological syndromes included stroke (n = 14), encephalitis (n = 12), encephalopathy (n = 11), transverse myelitis (n = 6) and Guillain-Barré syndrome (n = 4). Conclusions: Neurological involvement is common (48.63%) in COVID-19 patients within the first two weeks of the illness. This includes neurological symptoms such as anosmia, headaches, as well as a constellation of neurological syndromes such as stroke, encephalitis, transverse myelitis, and Guillain-Barré syndrome. Severity of acute COVID-19 illness and older age are the main risk factors.
Understanding the Hiring Issues of the Craft Workers in the UAE’s Construction Labor Market: Project Managers Perspective
Authors: Albattah Mohammed , Shibeika Amna , Sami Ur Rehman Muhammad
Published: January 2022

The construction industry in the United Arab Emirates (UAE) is faced with negative project performance, including low productivity, higher costs, delays in project completion, and defects during construction. One of the main reasons for these issues is the engagement of unskilled craft workers. Despite these labor issues, demand for construction projects in the UAE has been explosively increasing, in part due to Dubai winning the hosting of Expo 2020, which has given rise to an unprecedented demand for skilled construction craft workers. This study aimed to investigate the views and experiences of construction project managers regarding the reasons and challenges associated with the hiring of skilled craft workers in the construction labor market of the UAE. To fulfill the study purpose, the authors conducted structured open-ended interviews with UAE construction project managers. The results revealed several reasons for hiring craft workers despite a clear lack of qualifications and suggest some potential solutions.
Lockout, Lockdown and Land Use: Exploring the Spatio-Temporal Evolution Patterns of Licensed Venues in Sydney, Australia between 2012 and 2021 in the Context of NSW Public Policy
Authors: Perry Jayden Mitchell , Shirowzhan Sara , Pettit Christopher James
Published: January 2022

The hospitality industry in Sydney, Australia, has been subject to several regulatory interventions in the last decade, including lockout laws, COVID-19 lockdowns and land use planning restrictions. This study has sought to explore the spatial implications of these policies in Inner Sydney between 2012 to 2021. Methods based in spatial analysis were applied to a database of over 40,000 licensed venues. Point pattern analysis and spatial autocorrelation methods were used to identify spatially significant venue clusters. Space-time cube and emerging-hot-spot methods were used to explore clusters over time. The results indicate that most venues are located in the Sydney CBD on business-zoned land and show a high degree of spatial clustering. Spatio-temporal analysis reveals this clustering to be consistent over time, with variations between venue types. Venue numbers declined following the introduction of the lockout laws, with numbers steadily recovering in the following years. There was no discernible change in the number of venues following the COVID-19 lockdowns; however, economic data suggest that there has been a decline in revenue. Some venues were identified as having temporarily ceased trading, with these clustered in the Sydney CBD. The findings of this study provide a data-driven approach to assist policymakers and industry bodies in better understanding the spatial implications of policies targeting the hospitality sector and will assist with recovery following the COVID-19 pandemic. Further research utilising similar methods could assess the impacts of further COVID-19 lockdowns as experienced in Sydney in 2021.
Flexural Strength Evaluation of Multi-Cell Composite L-Shaped Concrete-Filled Steel Tubular Beams
Authors: Shen Yanfei , Tu Yongqing , Huang Wei
Published: January 2022

Concrete-filled steel tubular (CFST) members have been widely used in industrial structures and high-rise residential buildings. The multi-cell composite L-shaped concrete-filled steel tubular (ML-CFST) cross-section, as an innovative, special-shaped structural arrangement, may solve the issue of normal CFST members protruding from walls and result in more usable interior space. Currently, no design rules are available for the application of ML-CFST members. One of the primary objectives of the present study is to develop recommendations in line with the unified theory to evaluate the bending moment resistance of ML-CFST beams. According to the unified theory, the bending moment resistance of an ML-CFST beam is related to the compressive strength (fsc) and the flexural strength index (γm) of a composite section, in which the accuracy of γm and fsc are affected by a confinement effect factor (ξ). Nevertheless, the original expression of ξ is not suitable for ML-CFST sections, since the appreciable effect of the irregular shape on confinement is neglected. Considering the cross-sectional geometry and boundary conditions of the cells, an equivalent shape factor to modify the confinement effect was proposed in this study through dividing the infill concrete into highly confined areas and less confined areas. An adequate formula to calculate the fsc and an approximate expression of γm for the ML-CFST sections was then developed. Furthermore, four-point bending tests on eight specimens were carried out to investigate the flexural performance of the ML-CFST beams. Lastly, the proposed formulas were assessed against experimental and numerical results. The comparisons show that the proposed unified theory-based approach produced accurate and generally conservative results for the ML-CFST beams studied.
Photocatalytic Performance of Carbon-Containing CuMo-Based Catalysts under Sunlight Illumination
Authors: Muñoz-Flores Paula , Poon Po S. , Sepulveda Catherine , Ania Conchi O. , Matos Juan
Published: January 2022

Carbon-doped nanostructured CuMo-based photocatalysts were prepared by solvothermal synthesis. Two thermal treatments—oxidative and inert atmosphere—were used for the synthesis of the catalysts, and the influence of spherical carbon structures upon the crystalline phases on the photocatalytic activity and stability was studied. XRD showed the catalysts are nanostructured and composed by a mixture of copper (Cu, Cu2O, and CuO) and molybdenum (MoO2 and MoO3) crystalline phases. The catalysts were used for the degradation of yellow 5 under solar light. A remarkable leaching of Mo both in dark and under solar irradiation was observed and quantified. This phenomenon was responsible for the loss of photocatalytic activity for the degradation of the dye on the Mo-containing series. Conversely, the Cu-based photocatalysts were stable, with no leaching observed after 6 h irradiation and with a higher conversion of yellow 5 compared with the Mo- and CuMo series. The stability of Cu-based catalysts was attributed to a protective effect of spherical carbon structures formed during the solvothermal synthesis. Regarding the catalysts’ composition, sample Cu4-800-N2 prepared by pyrolysis exhibited up to 4.4 times higher photoactivity than that of the pristine material, which is attributed to a combined effect of an enhanced surface area and micropore volume generated during the pyrolytic treatment due to the presence of the carbon component in the catalyst. Scavenger tests have revealed that the mechanism for tartrazine degradation on irradiated Cu-based catalysts involves successive attacks of •OH radicals.
Chiral Ionic Liquids Based on l-Cysteine Derivatives for Asymmetric Aldol Reaction
Authors: Zalewska Karolina , Zakrzewska Małgorzata E. , Branco Luis C.
Published: January 2022

Structure, and consequently properties, of ionic liquids can be easily tailored by changing cation/anion combinations and/or attaching functional groups. By grafting enantiopure moieties to the framework of ionic liquid it is possible to prepare bioinspired chiral molecules that can serve as a reaction medium, additive or even asymmetric catalyst. In this context, new chiral ionic liquids (CILs), based on biomolecules, such as aminoacids (l-Cysteine derivatives), have been synthesised and tested in asymmetric aldol condensation of aldehydes and ketones. The best results were obtained for CILs composed of S-methyl-l-cysteine cation and bis(trifluoromethane)sulfonimide anion, in the reaction of 2- or 4-nitrobenzaldehyde with acetone or cyclohexanone, giving the aldol product in moderate yields 70–76% and high ee values (up to 96%).
Eucalyptol, an All-Purpose Product
Authors: Campos Joana F. , Berteina-Raboin Sabine
Published: January 2022

Eucalyptus plants have attracted the attention of researchers and environmentalists worldwide because they are a rapidly growing source of wood and a source of oil used for multiple purposes. The main and the most important oil component is 1,8-cineole (eucalyptol: 60–85%). This review summarizes the literature reported to date involving the use of 1,8-cineole for the treatment of disorders. Additionally, we describe our efforts in the use of eucalyptol as a solvent for the synthesis of O,S,N-heterocycles. Solvents used in chemistry are a fundamental element of the environmental performance of processes in corporate and academic laboratories. Their influence on costs, safety and health cannot be neglected. Green solvents such as bio-based systems hold considerable additional promise to reduce the environmental impact of organic chemistry. The first section outlines the process leading to our discovery of an unprecedented solvent and its validation in the first coupling reactions. This section continues with the description of its properties and characteristics and its reuse as reported in the various studies conducted. The second section highlights the use of eucalyptol in a series of coupling reactions (i.e., Suzuki–Miyaura, Sonogashira–Hagihara, Buchwald–Hartwig, Migita–Kosugi–Stille, Hiyama and cyanation) that form O,S,N-heterocycles. We describe the optimization process applied to reach the ideal conditions. We also show that eucalyptol can be a good alternative to build heterocycles that contain oxygen, sulfur and nitrogen. These studies allowed us to demonstrate the viability and potential that bio solvents can have in synthesis laboratories.
An Overview of the Latest Advances in the Catalytic Synthesis of Glycerol Carbonate
Authors: Procopio Debora , Di Gioia Maria Luisa
Published: January 2022

In recent years, the development of renewable energy alternatives to traditional fossil fuels has become one of the major challenges all over the world, due to the decline of fossil fuel reserves and their effect on global warming. Biodiesel has become a popular alternative energy source to reduce gas emissions compared to traditional fossil fuels. According to statistics, a nine-fold increase in global biofuel production between 2000 and 2020 was observed. However, its production generates a large amount of glycerol as a by-product, posing an environmental problem when disposed directly in landfills or by incineration. Therefore, low-value glycerol should be converted into high value-added derivatives. As glycerol carbonate is one of the most important derivatives of glycerol, this review aims to discuss the studies over the last ten years about glycerol carbonate synthetic methods, including the typical routes such as phosgene, esterification reaction, urea, oxidative and direct carbonylation as well as several rare synthetic procedures. At the same time, it summarizes the different catalytic reaction systems of each route comparing the advantages and disadvantages of various catalysts and evaluating their catalytic activity. Finally, the future development of glycerol carbonate synthesis is prospected from the point of view of development, technology research and industrialization.
Influence of Substrate Concentration on Kinetic Parameters of Ethanol Dehydration in MFI and CHA Zeolites and Relation of These Kinetic Parameters to Acid–Base Properties
Authors: Čičmanec Pavel , Kotera Jiří , Vaculík Jan , Bulánek Roman
Published: January 2022

The catalytic activity of zeolites is often related to their acid–base properties. In this work, the relationship between the value of apparent activation energy of ethanol dehydration, measured in a fixed bed reactor and by means of a temperature-programmed surface reaction (TPSR) depending on the amount of ethanol in the zeolite lattice and the value of activation energy of H/D exchange as a measure of acid–base properties of MFI and CHA zeolites, was studied. Tests in a fixed bed reactor were unable to provide reliable reaction kinetics data due to internal diffusion limitations and rapid catalyst deactivation. Only the TPSR method was able to provide activation energy values comparable to the activation energy values obtained from the H/D exchange rate measurements. In addition, for CHA zeolite, it has been shown that the values of ethanol dehydration activation energies depend on the amount of ethanol in the CHA framework, and this effect can be attributed to the substrate clustering effects supporting the deprotonation of zeolite Brønsted centers.
Hyaluronan: A Neuroimmune Modulator in the Microbiota-Gut Axis
Authors: Bosi Annalisa , Banfi Davide , Bistoletti Michela , Moretto Paola , Moro Elisabetta , Crema Francesca , Maggi Fabrizio , Karousou Evgenia , Viola Manuela , Passi Alberto , Vigetti Davide , Giaroni Cristina , Baj Andreina
Published: January 2022

The commensal microbiota plays a fundamental role in maintaining host gut homeostasis by controlling several metabolic, neuronal and immune functions. Conversely, changes in the gut microenvironment may alter the saprophytic microbial community and function, hampering the positive relationship with the host. In this bidirectional interplay between the gut microbiota and the host, hyaluronan (HA), an unbranched glycosaminoglycan component of the extracellular matrix, has a multifaceted role. HA is fundamental for bacterial metabolism and influences bacterial adhesiveness to the mucosal layer and diffusion across the epithelial barrier. In the host, HA may be produced and distributed in different cellular components within the gut microenvironment, playing a role in the modulation of immune and neuronal responses. This review covers the more recent studies highlighting the relevance of HA as a putative modulator of the communication between luminal bacteria and the host gut neuro-immune axis both in health and disease conditions, such as inflammatory bowel disease and ischemia/reperfusion injury.
Immunocytochemical Labelling of Haematological Samples Using Monoclonal Antibodies
Authors: Erber Wendy N.
Published: January 2022

I reflect on my experience working with David Y. Mason in the Leukaemia Research Laboratories in the Nuffield Department of Pathology at the University of Oxford in the early 1980s. This was soon after the first monoclonal antibodies had been produced, which led to an exciting and productive time in biological discovery and pathology diagnostics. A specific focus in the laboratory was the development of immunoenzymatic staining methods that would enable monoclonal antibodies to be applied in diagnostic practice. This paper describes the work that led to the performance of immuno-alkaline phosphatase staining on blood and bone marrow smears, the success of which changed leukaemia diagnosis.
Differences in Immune-Related Genes Underlie Temporal and Regional Pathological Progression in 3xTg-AD Mice
Authors: Fernandes Adelaide , Caldeira Cláudia , Cunha Carolina , Ferreiro Elisabete , Vaz Ana Rita , Brites Dora
Published: January 2022

The prevalence of Alzheimer’s disease (AD), the most common cause of age-associated dementia, is estimated to increase over the next decades. Evidence suggests neuro-immune signaling deregulation and risk genes beyond the amyloid-β (Aβ) deposition in AD pathology. We examined the temporal profile of inflammatory mediators and microglia deactivation/activation in the brain cortex and hippocampus of 3xTg-AD mice at 3- and 9-month-old. We found upregulated APP processing, decreased expression of CD11b, CX3CR1, MFG-E8, TNF-α, IL-1β, MHC-II and C/EBP-α and increased miR-146a in both brain regions in 3-month-old 3xTG-AD mice, suggestive of a restrictive regulation. Enhanced TNF-α, IL-1β, IL-6, iNOS, SOCS1 and Arginase 1 were only present in the hippocampus of 9-month-old animals, though elevation of HMGB1 and reduction of miR-146a and miR-124 were common features in the hippocampus and cortex regions. miR-155 increased early in the cortex and later in both regions, supporting its potential as a biomarker. Candidate downregulated target genes by cortical miR-155 included Foxo3, Runx2 and CEBPβ at 3 months and Foxo3, Runx2 and Socs1 at 9 months, which are implicated in cell survival, but also in Aβ pathology and microglia/astrocyte dysfunction. Data provide new insights across AD state trajectory, with divergent microglia phenotypes and inflammatory-associated features, and identify critical targets for drug discovery and combinatorial therapies.
Amino Acid Metabolism in Cancer Drug Resistance
Authors: Yoo Hee-Chan , Han Jung-Min
Published: January 2022

Despite the numerous investigations on resistance mechanisms, drug resistance in cancer therapies still limits favorable outcomes in cancer patients. The complexities of the inherent characteristics of tumors, such as tumor heterogeneity and the complicated interaction within the tumor microenvironment, still hinder efforts to overcome drug resistance in cancer cells, requiring innovative approaches. In this review, we describe recent studies offering evidence for the essential roles of amino acid metabolism in driving drug resistance in cancer cells. Amino acids support cancer cells in counteracting therapies by maintaining redox homeostasis, sustaining biosynthetic processes, regulating epigenetic modification, and providing metabolic intermediates for energy generation. In addition, amino acid metabolism impacts anticancer immune responses, creating an immunosuppressive or immunoeffective microenvironment. A comprehensive understanding of amino acid metabolism as it relates to therapeutic resistance mechanisms will improve anticancer therapeutic strategies.
Complexation Behavior of Pinene–Bipyridine Ligands towards Lanthanides: The Influence of the Carboxylic Arm
Authors: Solea Atena B. , Yang Liangru , Crochet Aurelien , Fromm Katharina M. , Allemann Christophe , Mamula Olimpia
Published: January 2022

The complexation behavior of two novel, chiral pinene–bipyridine-type ligands ((–)-HL1 and (–)-HL2) containing a carboxylic arm towards lanthanide Ln(III) (Ln = La, Eu, Lu) ions was investigated through spectroscopic methods. The association constants of the mononuclear complexes determined from the UV-Vis titrations indicated that the ligand (–)-HL1 possessing a shorter carboxylic arm formed more stable complexes compared with (–)-HL2, whose carboxylic arm had one more methylene unit. This is due to the formation of more stable seven-member metal chelate rings in the first case as compared with the eight-member metal chelate rings in the second. IR and fluorescence spectroscopy provided additional information about the structure of these complexes.
Recent Development of Optofluidics for Imaging and Sensing Applications
Authors: Tang Jiukai , Qiu Guangyu , Wang Jing
Published: January 2022

Optofluidics represents the interaction of light and fluids on a chip that integrates microfluidics and optics, which provides a promising optical platform for manipulating and analyzing fluid samples. Recent years have witnessed a substantial growth in optofluidic devices, including the integration of optical and fluidic control units, the incorporation of diverse photonic nanostructures, and new applications. All these advancements have enabled the implementation of optofluidics with improved performance. In this review, the recent advances of fabrication techniques and cutting-edge applications of optofluidic devices are presented, with a special focus on the developments of imaging and sensing. Specifically, the optofluidic based imaging techniques and applications are summarized, including the high-throughput cytometry, biochemical analysis, and optofluidic nanoparticle manipulation. The optofluidic sensing section is categorized according to the modulation approaches and the transduction mechanisms, represented by absorption, reflection/refraction, scattering, and plasmonics. Perspectives on future developments and promising avenues in the fields of optofluidics are also provided.
The Ionic Associate of Metamizole as an Electrode-Active Component of a PVC Plasticized Membrane Electrode
Authors: Tataeva Sarizhat D. , Magomedov Kurban E. , Zeynalov Ruslan Z. , Baygishieva Naida D. , Magomedova Viktorya S. , Rabadanova Alina A. , Orudzhev Farid F.
Published: January 2022

The technology for manufacturing a film membrane of the metamizole-selective electrode containing ion associate metamizole-octadecylammonium ODAH+MT− as an electrode active component (EAC) has been proposed. The main potentiometric characteristics of the metamizole-selective electrode have been determined. The expediency of the proposed design of the metamizole selective electrode for the determination of metamizole in dosage forms has been substantiated. The best composition of the membrane (wt.%) of the metamizole-selective electrode has corresponded to: ODAH+MT−—5.3; 2-nitrophenyloctylether—63.1; poly(vinyl chloride)—31.6. Electrode-active component in the membrane phase functions as an ion associate ODAH+MT−. Potentiometric characteristics of metamizole-selective electrode have been determined, which corresponded to: linear range 1 × 10−2–1 × 10−4 with limit of detection 4.58 × 10−5 M, electrode function slope −48.5 mV/dec., working interval pH 4.5–7.3, response time 60 s. The potentiometric coefficients of selectivity of the metamizole-selective electrode with respect to various ions have been determined. The possibility of determining metamizole in a medicinal product has been tested. The results of the analyses show good agreement between the two methods (relative error less than 7.0%) with coefficients of variation less than 5% for MT-SE and iodometric methods.
A Bayesian Framework to Assess the Usability of Dry Powder Inhalers in a Cohort of Asthma Adolescents in Italy
Authors: Dal Negro Roberto Walter , Povero Massimiliano
Published: January 2022

The useability of DPIs (dry powder inhalers) depends on several factors that are influenced by the patients’ subjectivity and objectivity. The short-form global usability score (S-GUS), a specific tool for the quick ranking and comparison in real life of an inhaler’s usability, was used to investigate six of the most prescribed DPIs (Breezhaler, Diskus, Ellipta, Nexthaler, Spiromax, and Turbohaler) in consecutive asthma patients aged <18 years. A Bayesian indirect comparison (IC) was carried out to merge all pairwise comparisons between the six DPIs. Thirty-three subjects participated: eighteen tested Breezhaler, Spiromax, Nexthaler, and Ellipta simultaneously, while fifteen tested Breezhaler, Spiromax, Diskus, and Turbohaler. The estimates of the S-GUS, by the IC model, allowed us to rank the DPIs by their degree of usability: Ellipta, Diskus, and Spiromax were classified as “good to pretty good” (S-GUS > 15), while Spiromax, Turbohaler, and Breezhaler were classified as “insufficient” (S-GUS < 15). The multidomain assessment is recommended in asthma adolescents in order to approximate the effective usability of different DPIs as best as possible. The S-GUS proves particularly suitable in current clinical practice because of the short time required for its use in adolescents.
Pediatric Training Crisis of Emergency Medicine Residency during the COVID-19 Pandemic
Authors: Huang Yan-Bo , Lin Yu-Ru , Hung Shang-Kai , Chang Yu-Che , Ng Chip-Jin , Chen Shou-Yen
Published: January 2022

Coronavirus disease 2019 (COVID-19) is an emerging viral disease that has caused a global pandemic. Among emergency department (ED) patients, pediatric patient volume mostly and continuously decreased during the pandemic period. Decreased pediatric patient volume in a prolonged period could results in inadequate pediatric training of Emergency Medicine (EM) residents. We collected data regarding pediatric patients who were first seen by EM resident physicians between 1 February 2019, and 31 January 2021, which was divided into pre-epidemic and epidemic periods by 1 February 2020. A significant reduction in pediatric patients per hour (PPH) of EM residents was noted in the epidemic period (from 1.55 to 0.81, p < 0.001). The average patient number was reduced significantly in the classification of infection (from 9.50 to 4.00, p < 0.001), respiratory system (from 84.00 to 22.00, p < 0.001), gastrointestinal system (from 52.00 to 34.00, p = 0.007), otolaryngology (from 4.00 to 2.00, p = 0.022). Among the diagnoses of infectious disease, the most obvious drop was noted in the diagnosis of influenza and enterovirus infection. Reduced pediatric patient volume affected clinical exposure to pediatric EM training of EM residency. Changes in the proportion of pediatric diseases presented in the ED may induce inadequate experience with common and specific pediatric diseases.
The Influence of Parent Media Use, Parent Attitude on Media, and Parenting Style on Children’s Media Use
Authors: Lee Hye Eun , Kim Ji Young , Kim Changsook
Published: January 2022

Parents play a vital role in mediating children’s media use, especially at a young age. We examined the link between the media use of younger children and the media use, attitude toward media, and parenting styles of parents. One thousand and twenty parents of children between 4 and 6 years of age completed a questionnaire on their media use, positive and negative attitudes on media, parenting styles, and the media use of their children. Multigroup structural equation modeling was used to analyze the data. The results showed that there was a significant positive relation between the parent’s media time and the child’s daytime and nighttime media use. Additionally, the parent’s positive attitude toward media use was positively related to the child’s daytime media use, but not the child’s nighttime media use, while the parent’s negative attitude toward media was not associated with the child’s daytime and nighttime media use. Further, among the seven parenting styles, material rewards and autonomy were positively associated with the child’s daytime media use. Discipline was negatively related to the child’s nighttime media use, whereas material rewards were positively associated with the child’s nighttime media use. Collectively, the parent’s positive attitude toward media use was the strongest predictor of the child’s daytime media use, and material rewards were the strongest predictor of the child’s nighttime media use. These results can be of significant use to inform policymakers, researchers, and parents regarding the development of parental guidelines on children’s media use.