bluuebunny/crossref_metadata_2025 huggingface dataset:
Silencing of Glycogen Synthase Kinase 3 Significantly Inhibits Chitin and Fatty Acid Metabolism in Asian Citrus Psyllid, Diaphorina citri
Authors: Zhang Jin-Bo , Lu Zhan-Jun , Yu Hai-Zhong
Published: August 2022

Glycogen is a predominant carbohydrate reserve in various organisms, which provides energy for different life activities. Glycogen synthase kinase 3 (GSK3) is a central player that catalyzes glucose and converts it into glycogen. In this study, a GSK3 gene was identified from the D. citri genome database and named DcGSK3. A reverse transcription quantitative PCR (RT-qPCR) analysis showed that DcGSK3 was expressed at a high level in the head and egg. The silencing of DcGSK3 by RNA interference (RNAi) led to a loss-of-function phenotype. In addition, DcGSK3 knockdown decreased trehalase activity, glycogen, trehalose, glucose and free fatty acid content. Moreover, the expression levels of the genes associated with chitin and fatty acid synthesis were significantly downregulated after the silencing of DcGSK3. According to a comparative transcriptomics analysis, 991 differentially expressed genes (DEGs) were identified in dsDcGSK3 groups compared with dsGFP groups. A KEGG enrichment analysis suggested that these DEGs were primarily involved in carbon and fatty acid metabolism. The clustering analysis of DEGs further confirmed that chitin and fatty acid metabolism-related DEGs were upregulated at 24 h and were downregulated at 48 h. Our results suggest that DcGSK3 plays an important role in regulating the chitin and fatty acid metabolism of D. citri.
RNA Editing Enzyme ADAR1 Regulates METTL3 in an Editing Dependent Manner to Promote Breast Cancer Progression via METTL3/ARHGAP5/YTHDF1 Axis
Authors: Li Yi , Wang Ning-Xi , Yin Chuan , Jiang Shan-Shan , Li Jia-Chu , Yang Sheng-Yong
Published: August 2022

A-to-I RNA editing and m6A modification are two of the most prevalent types of RNA modifications controlling gene expression in mammals and play very important roles in tumorigenesis and tumor progression. However, the functional roles and correlations of these two RNA modifications remain to be further investigated in cancer. Herein, we show that ADAR1, an A-to-I RNA-editing enzyme, interacts with METTL3 and increases its protein level to promote the proliferation, migration and invasion of breast cancer cells through a mechanism connecting ADAR1, METTL3 and YTHDF1. We show that both ADAR1 and METTL3 are upregulated in breast cancer samples, and ADAR1 positively correlates with METTL3; ADAR1 edits METTL3 mRNA and changes its binding site to miR532-5p, leading to increased METTL3 protein, which further targets ARHGAP5, recognized by YTHDF1. Additionally, we show that loss of ADAR1 significantly inhibits breast cancer growth in vivo. Collectively, our findings identify the ADAR1–METTL3 axis as a novel, important pathway that connects A-to-I editing and m6A RNA modifications during breast cancer progression.
Quinolizidines as Novel SARS-CoV-2 Entry Inhibitors
Authors: Huang Li , Zhu Lei , Xie Hua , Goodwin Jeffery Shawn , Rana Tanu , Xie Lan , Chen Chin-Ho
Published: August 2022

COVID-19, caused by the highly transmissible severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has rapidly spread and become a pandemic since its outbreak in 2019. We have previously discovered that aloperine is a new privileged scaffold that can be modified to become a specific antiviral compound with markedly improved potency against different viruses, such as the influenza virus. In this study, we have identified a collection of aloperine derivatives that can inhibit the entry of SARS-CoV-2 into host cells. Compound 5 is the most potent tested aloperine derivative that inhibited the entry of SARS-CoV-2 (D614G variant) spike protein-pseudotyped virus with an IC50 of 0.5 µM. The compound was also active against several other SARS-CoV-2 variants including Delta and Omicron. Results of a confocal microscopy study suggest that compound 5 inhibited the viral entry before fusion to the cell or endosomal membrane. The results are consistent with the notion that aloperine is a privileged scaffold that can be used to develop potent anti-SARS-CoV-2 entry inhibitors.
Predictors of Long COVID in Patients without Comorbidities: Data from the Polish Long-COVID Cardiovascular (PoLoCOV-CVD) Study
Authors: Chudzik Michał , Lewek Joanna , Kapusta Joanna , Banach Maciej , Jankowski Piotr , Bielecka-Dabrowa Agata
Published: August 2022

Background: The SARS-CoV-2 pandemic has become an enormous worldwide challenge over the last two years. However, little is still known about the risk of Long COVID (LC) in patients without comorbidities. Thus, we aimed to assess the predictors of LC in patients without comorbidities. Methods: Patients’ information, the course of the disease with symptoms, and post-COVID-19 complaints were collected within 4–12 weeks after COVID-19 recovery. Next, the patients were followed for at least 3 months. ECG, 24-h ECG monitoring, 24-h blood pressure (BP) monitoring, echocardiography, and selected biochemical tests were performed. LC was recognized based on the WHO definition. Results: We identified 701 consecutive patients, 488 of whom completed a 3-month follow-up (63% women). Comparisons were made between the LC group (n = 218) and patients without any symptoms after SARS-CoV-2 recovery (non-LC group) (n = 270). Patients with a severe course of acute-phase COVID-19 developed LC complications more often (34% vs. 19%, p < 0.0001). The persistent symptoms were observed in 45% of LC patients. The LC group also had significantly more symptoms during the acute phase of COVID-19, and they suffered significantly more often from dyspnoea (48 vs. 33%), fatigue (72 vs. 63%), chest pain (50 vs. 36%), leg muscle pain (41 vs. 32%), headache (66 vs. 52%), arthralgia (44 vs. 25%), and chills (34 vs. 25%). In LC patients, significant differences regarding sex and body mass index were observed—woman: 69% vs. 56% (p = 0.003), and BMI: 28 [24–31] vs. 26 kg/m2 [23–30] (p < 0.001), respectively. The number of symptoms in the acute phase was significantly greater in the LC group than in the control group (5 [2–8] vs. 2 [1–5], p = 0.0001). The LC group also had a higher 24-h heart rate (77 [72–83] vs. 75 [70–81], p = 0.021) at admission to the outpatient clinic. Multivariate regression analysis showed that LC patients had a higher BMI (odds ratio (OR): 1.06, 95% confidence intervals [CI]: 1.02–1.10, p = 0.007), almost twice as often had a severe course of COVID-19 (OR: 1.74, CI: 1.07–2.81, p = 0.025), and presented with joint pain in the acute phase (OR: 1.90, CI: 1.23–2.95, p = 0.004). Conclusions: A severe course of COVID-19, BMI, and arthralgia are independently associated with the risk of Long COVID in healthy individuals.
Fracture Load and Fracture Patterns of Monolithic Three-Unit Anterior Fixed Dental Prostheses after In Vitro Artificial Aging—A Comparison between Color-Gradient and Strength-Gradient Multilayer Zirconia Materials with Varying Yttria Content
Authors: Pöppel Max L. , Rosentritt Martin , Sturm Richard , Beuer Florian , Hey Jeremias , Schmid Alois , Schmidt Franziska
Published: August 2022

(1) Background: Due to advantages such as avoidance of chipping, pulp-friendly tooth preparation and cost reduction, zirconia is increasingly being used monolithically without veneering. Nevertheless, to enable good aesthetics, various multilayer systems have been developed. The aim of this study was to investigate the impact of different zirconia multilayer strategies and yttria levels on fracture load, fracture pattern, stress distribution and surface roughness. (2) Methods: Monolithic three-unit anterior FDPs were made from three different color-gradient zirconia multilayer materials with different yttria levels corresponding to varying strength and degrees of translucency grades (Katana HTML, STML, UTML, Kuraray) and one strength-gradient zirconia multilayer material (Katana YML, Kuraray) and artificially aged in a chewing simulator (1.2 × 106 load cycles, 50 N, 2 × 3000 thermocycles, 5–55 °C). Analyses of fracture load, fracture pattern, fracture surfaces, stress distribution and roughness were performed after the fracture load test. Shapiro–Wilk, Kruskal–Wallis, Mann–Whitney U-tests and one-way ANOVA were used (p < 0.05). (3) Results: Fracture loads of the high strength color-gradient material HTML and the strength-gradient material YML were comparable after 5 years of aging (p = 0.645). Increasing yttria levels resulted in a decrease in fracture resistance of 42–57% (p < 0.05). Surface roughness of different zirconia generations is comparable after polishing and aging. (4) Conclusions: Color-gradient multilayer zirconia materials and new strength-gradient zirconia materials with similar yttria levels in the basal layers show comparable mechanical properties and are suitable for anterior FDPs.
Factors Influencing Longevity of Humoral Response to SARS-CoV-2 Vaccination in Patients with End Stage Kidney Disease Receiving Renal Replacement Therapy
Authors: Glowinska Irena , Labij-Reduta Barbara , Juzwiuk Jerzy , Lukaszewicz Magdalena , Pietruczuk Adam , Poplawska Agata , Daniluk-Jamro Anna , Kakareko Katarzyna , Rydzewska-Rosolowska Alicja , Naumnik Beata , Koc-Zorawska Ewa , Zorawski Marcin , Hryszko Tomasz
Published: August 2022

COVID-19 has severely affected the population of patients with end stage renal disease. Current data have proved a two-dose vaccination schedule against SARS-CoV-2 to be effective among dialyzed patients. There are limited data on the longevity and modulating factors of humoral response after vaccination. We performed a prospective longitudinal cohort study to determine longevity of the humoral response after SARS-CoV-2 vaccine. The study included 191 adult patients on hemodialysis and peritoneal dialysis. All participants had been vaccinated with three doses, either with BNT162b2 (Pfizer-BioNTech) (n = 109) or mRNA-1273 (Moderna) (n = 82). Anti-spike protein receptor-binding domain antibodies (anti-S IgG) were assessed using SARS-CoV-2 (RBD) IgG ELISA EIA-6150 IVD assay at baseline, on the 21st day and 43rd day, before a booster dose and two weeks thereafter. We found that before vaccination, 37.7% of the cohort had anti-S IgG titres concordant with seroconversion. After two-dose vaccination, seroconversion occurred in 97% of patients. The booster dose evoked a ~12-fold increase in antibody level. Obesity increased more than two-fold the odds for a decrease in anti-S IgG. Previous COVID-19 infection enhanced longevity of the humoral response following vaccination. In patients with previous COVID-19 infection, the BNT162b2 vaccine was associated with a higher odds of anti-S IgG waning compared to the mRNA-1273 vaccine. In conclusion, we report that obesity predisposes patients to protective antibody waning, hybrid immunity enhances odds for higher anti-S IgG concentrations and vaccine efficacy may be influenced by previous SARS-CoV-2 infection. The results might provide a rationale for vaccination protocol design.
Psychological Consequences of Experiencing Violence in Childhood—The Role of Dissociation in the Formation of Early Maladaptive Schemas
Authors: Sieński Michał M. , Ziarko Michał
Published: August 2022

Childhood experiences of violence can lead to severe psychological consequences. One of them is an increased risk of abnormal personality development. It can manifest as rigid negativistic beliefs about the self, others, and the surrounding world, which some specialists term early maladaptive schemas. The conducted study aims to provide a possible explanation of the role dissociation plays in the process of forming early maladaptive schemas. The study was conducted on 342 adult women whose biographies contained one or more episodes of potentially traumatic experiences of violence in childhood. Three questionnaires were used: Childhood Trauma Questionnaire, Dissociative Experiences Scale, Young Schema Questionnaire. The results show that experiences of violence are linked with dissociative disorders and the intensity of early maladaptive schemas. Mediation analysis confirmed that the relationship between experiencing violence and early maladaptive schemas is exacerbated by the presence of dissociative symptoms resulting from the violence experienced. The mediation analysis results suggest that if an experience of violence is followed by the emergence of dissociative symptoms, its impact on personality is more severe. This confirms earlier notions regarding the role that dissociative symptoms play in personality changes stemming from traumatic events.
A Comparison of the Catheter-Based Transapical and Surgical Treatment Modalities for Mitral Paravalvular Leak
Authors: Zorinas Aleksejus , Janušauskas Vilius , Austys Donatas , Davidavičius Giedrius , Puodžiukaitė Lina , Zakarkaitė Diana , Samalavičius Robertas , Urbonas Karolis , Kramena Rita , Onorato Eustaquio , Ručinskas Kęstutis
Published: August 2022

Background: There is a lack of studies where the outcomes of mitral paravalvular leak treatment were compared between surgery and catheter-based closure. The aim of this study was to compare the outcomes of re-do surgery with transapical catheter-based paravalvular leak closure. Methods: This is a retrospective observational study at a single institution; 76 patients were included. According to the treatment, two groups were formed: the “Surgical” group (49 patients after re-do surgery) and the “Catheter” group (27 patients after transapical catheter–based treatment). Results: In-hospital myocardial infarction occurred in 9 (18%) cases in the “Surgical” group and none in the “Catheter” group, p = 0.018. Procedure-related life-threatening bleeding occurred in 9 (18%) patients in the “Surgical” group and none in the “Catheter” group, p = 0.018. Nine (18%) patients died in 30 days in the “Surgical” group, and none died in the “Catheter” group, p = 0.039. A mean follow-up was 3.3 years. No difference was found between the groups by the degree of residual paravalvular regurgitation either at discharge or at follow-up. During the follow-up, 19 (39%) patients died in the “Surgical” group and 2 (7%) among the “Catheter” patients. Conclusions: Transapical catheter-based closure of mitral paravalvular leak seems to be a safer treatment procedure than conventional re-do surgery, and the effectiveness of these procedures does not differ.
Functional Roles of LaeA-like Genes in Fungal Growth, Cellulase Activity, and Secondary Metabolism in Pleurotus ostreatus
Authors: Zhang Guang , Yan Peng , Leng Doudou , Shang Li , Zhang Chaohui , Wu Zhongwei , Wang Zhenhe
Published: August 2022

The global regulator LaeA plays crucial roles in morphological development and secondary metabolite biosynthesis in filamentous fungi. However, the functions of LaeA in basidiomycetes are less reported. The basidiomycete Pleurotus ostreatus is a well-known fungus used both in medicine and as food that produces polysaccharides and cellulolytic enzymes. In this study, we characterized three LaeA homologs (PoLaeA1, PoLaeA2, and PoLaeA3) in P. ostreatus. PoLaeA1 showed different expression patterns than PoLaeA2 and PoLaeA3 during different developmental stages. Silencing PoLaeA1 decreased the intracellular polysaccharide (IPS) content by approximately 28–30% and reduced intracellular ROS levels compared with those of the WT strain. However, silencing PoLaeA2 and PoLaeA3 decreased cellulase activity by 31–34% and 35–40%, respectively, and reduced the cytosolic Ca2+ content, compared with those of the WT strain. Further analysis showed that PoLaeA1 regulated IPS biosynthesis through intracellular ROS levels, whereas PoLaeA2 and PoLaeA3 regulated cellulase activity through intracellular Ca2+ signaling. Our results provide new insights into the regulation of polysaccharide biosynthesis and cellulase production in filamentous fungi.
A Preconditioning Paradox: Contrasting Effects of Initial Phyllosphere and Early Leaf Decomposer Microfungi on Subsequent Colonization by Leaf Decomposing Non-Unit-Restricted Basidiomycetes
Authors: Bibbo Silvia , Lodge D. Jean
Published: August 2022

Fungal interactions during leaf decomposition can facilitate or inhibit other fungi. This experiment focused on whether preconditioning of leaf litter by microfungi that were confined to one leaf (Unit-Restricted) made leaf litter less likely to be colonized and decomposed by basidiomycetes that bind litter into mats (Non-Unit-Restricted) than non-preconditioned litter. Leaves of Manilkara bidentata in litterbags were preconditioned by incubating them for 0, 1, 2 or 3 months in flat litter/seed rain baskets 10 cm above the forest floor to avoid colonization by basidiomycete fungi. Preconditioned and non-preconditioned leaves were transferred to 5 replicate basidiomycete fungal mats of Gymnopus johnstonii for 6 weeks. Both attachment by basidiomycete fungi and percent mass loss after 6 weeks decreased significantly with increasing preconditioning time. In non-preconditioned leaves, gamma irradiation did not affect mass loss or percent white-rot despite having significantly increased numbers of basidiomycete fungal connections as compared to non-irradiated leaves. In non-preconditioned leaves, more basidiomycetes attachmented to non-irradiated than irradiated leaves suggest facilitation by phyllosphere microfungi. While basidiomycete colonization was initially facilitated by phyllosphere fungi, we inferred that degradation of resource quality led to fewer fungal attachments and less mass loss after 1–3 months of preconditioning by microfungi. The date suggest there is a 1-month time window for basidiomycete fungi to incorporate fallen leaves into their litter mats.
Population Dynamics in China’s Urbanizing Megaregion: A Township-Level Analysis of the Beijing–Tianjin–Hebei Region
Authors: Wang Yanxi , Zhuo Yunxia , Liu Tao
Published: August 2022

China is currently in a period of accelerated urbanization, and the population pattern of urbanizing megaregions is undergoing drastic changes. Accurately grasping the population density patterns and evolution trends has become essential. Based on the township-level population data, through population density classification, population concentration index, and regression analysis, this research investigated the evolution of the spatial pattern of population density and the influencing factors in the Beijing–Tianjin–Hebei region. Results showed that the population continued to concentrate in the municipal districts of Beijing and Tianjin and the township units where county governments were located, thereby causing a more unbalanced population distribution and a wider urban–rural disparity. Population dynamics are influenced by the market and the government. County-level administrative centers have continued to appeal to the population. The strategy of decentralizing the non-capital functions of Beijing has promoted the decentraliztion of population, albeit to a limited extent. However, key township policy has played a minor role in population change. Owing to particularities in the development stage and social system, the population dynamics in the Beijing–Tianjin–Hebei region differ from those of other developed countries.
Spatial Coupling Coordination Evaluation between Population Growth, Land Use and Housing Supply of Urban Agglomeration in China
Authors: Kong Qingshan , Kong Haiyang , Miao Silin , Zhang Qin , Shi Jiangang
Published: August 2022

The spatial imbalance between population growth, land use and housing supply is the central issue for regional coordination of urban agglomeration in China. Based on the panel data of 172 cities in 11 urban agglomerations from 2014 to 2017, this study uses the information entropy method and the spatial coupling coordination degree model to evaluate the quantitative interaction and spatial correlations between population growth, land use and housing supply. There are three key findings: (1) the main variation value of indicators has evolved from the quantity of housing supply to the quality of population growth, improving the quality of population growth has been the key factor to break the insufficient balance of indicators; (2) the coupling degree is high but the coordination degree is obviously low, the aggregation level of coupling coordination degree is generally middle, and there is obviously spatial polarization—improving the degree of coordination is the key point to break the inadequate balance of cities; (3) the coupling coordination degree is irregularly distributed in 11 urban agglomerations, the spatial correlation of coupling coordination degree is generally weak, improving the spatial coordination degree of urban agglomeration will contribute to improving the balanced sufficiency level, and the spatial coupling coordination degree is also expected to increase. This study presents a new perspective for exploring spatial coordination between population growth, land use and housing supply, which proposes a new approach to investigate quantitative interaction and spatial correlation of urban agglomeration in China.
Photosynthesis, Nitrogen Allocation, Non-Structural Carbohydrate Allocation, and C:N:P Stoichiometry of Ulmus elongata Seedlings Exposed to Different Light Intensities
Authors: Luo Guangyu , Li Junmin , Guo Shuiliang , Li Yueling , Jin Zexin
Published: August 2022

The leaf photosynthetic capacity, leaf N partitioning, non-structural carbohydrate content, C, N, and P contents of endangered U. elongata seedlings exposed to different light intensities were compared in this study. The most favorable light condition for the survival and growth of U. elongata seedlings in the present study was 100% full sunlight, as this induced higher Pn, PNUE, PC, PR, PB, and NSC content relative to shade-treated seedlings. PNUE, PR, PC, and PB in U. elongata seedling leaves decreased under 40% and 10% full sunlight, while PL increased, indicating that shade increased the light capture efficiency of photosystem (PS) II but decreased electron transfer from PSII to PSI. Furthermore, leaf N content increased with shade intensity, revealing an adaptive strategy for poor light environments. Additionally, the smallest leaf biomass, Pn, WUE, and CE values and C:N and C:P ratios in stems and leaves were observed under 10% full sunlight. These results indicate that seedlings growing under 40% full sunlight will benefit U. elongata conservation.
Analysis of Electromagnetic Characteristics of Copper-Steel Composite Quadrupole Rail
Authors: Li Tengda , Feng Gang , Du Chong , Zhang Pengxiang
Published: August 2022

The ablation and wear of the four-rail electromagnetic launcher during the working process will aggravate the damage of the armature and rail, and greatly affect the service life of the launcher. To effectively alleviate rail damage, this paper applies the copper-steel composite rail to the four-rail electromagnetic launcher and proposes a new four-rail electromagnetic launcher. Based on the quadrupole magnetic field theory, the physical model of the new four-rail electromagnetic launcher is established, and the electromagnetic characteristics of the ordinary and new launchers are compared and analyzed using the finite element method. On this basis, the influence of composite layer parameters on the electromagnetic characteristics of copper-steel composite quadrupole rail is explored. The study found that the new four-rail electromagnetic launcher can provide a better launch magnetic field environment for smart loads, and the current distribution of the armature and the rail contact surface is more uniform, which can effectively improve the contact condition between the armature and the rail. The composite layer parameters of copper-based composite rail will have a certain impact on electromagnetic characteristics, and copper-steel composite rail of appropriate proportions can be selected according to different needs. The model proposed in this paper has a certain degree of scientificity and rationality.
Synergistic Effect of Cerium Oxide for Improving the Fire-Retardant, Mechanical and Ultraviolet-Blocking Properties of EVA/Magnesium Hydroxide Composites
Authors: Hobson Jose , Yin Guang-Zhong , Yu Xiaoli , Zhou Xiaodong , Prolongo Silvia Gonzalez , Ao Xiang , Wang De-Yi
Published: August 2022

Rare earth oxide particles have received important attention in recent years, and due to the wide diversity of promising applications, the need for this kind of material is predicted to expand as the requirements to use the current resources become more demanding. In this work, cerium oxide (CeO2) was introduced into ethylene-vinyl acetate (EVA)/magnesium hydroxide (MDH) composites for enhancing the flame retardancy, mechanical properties and anti-ultraviolet aging performance. The target EVA/MDH/CeO2 composites were prepared by extrusion and injection molding, and the effects of the addition of the CeO2 were explored by thermogravimetric analysis (TGA), Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD), limiting oxygen index (LOI), UL-94, cone calorimetry test, and anti-ultraviolet aging test. Typically, the incorporation of the CeO2 allows a significant increase of the elongation at break and Young’s modulus compared with EVA/MDH by 52.25% and 6.85%, respectively. The pHRR remarkably decreased from 490.6 kW/m2 for EVA/MDH to 354.4 kW/m2 for EVA/MDH/CeO2 composite. It was found that the CeO2 presents excellent synergism with MDH in the composites for the anti-UV properties in terms of mechanical properties preservation. Notably, the combination of CeO2 with MDH is a novel and simple method to improve the filler–polymer interaction and dispersion, which resulted in the improvement of the mechanical properties, flame retardancy and the anti-ultraviolet aging performance of the composites.
Determination of Nanoindentation Behavior of HAZ on Glass Material Machined via ECSM Process through Simulation Approach
Authors: Singh Tarlochan , Sharma Sahil , Sidhu Sarabjeet Singh , Shlykov Evgeny Sergeevich , Ablyaz Timur Rizovich
Published: August 2022

The current study develops a numerical model to investigate the nanoindentation behavior of heat-affected zones (HAZ) on glass material produced via the electrochemical spark machining (ECSM) method. Initially, microchannels were created using the ECSM method on soda–lime glass. Following that, a nanoindentation test was conducted to quantify the Young’s modulus and hardness of the glass sample. After that, a numerical model based on finite elements was created to characterize the changes in mechanical characteristics of HAZ. According to the findings, increasing the electrolyte concentration from 10 to 30% increases the intensity of electrochemical discharges, and thereby decreases the hardness of the work material by 16.29 to 30.58% compared to unmachined glass. The results obtained from the simulation are in close agreement with the experimental values. The maximum error obtained between simulation and experimental results is only 4.18%.
Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications
Authors: Binaymotlagh Roya , Chronopoulou Laura , Haghighi Farid Hajareh , Fratoddi Ilaria , Palocci Cleofe
Published: August 2022

Peptide-based hydrogels have attracted increasing attention for biological applications and diagnostic research due to their impressive features including biocompatibility and biodegradability, injectability, mechanical stability, high water absorption capacity, and tissue-like elasticity. The aim of this review will be to present an updated report on the advancement of peptide-based hydrogels research activity in recent years in the field of anticancer drug delivery, antimicrobial and wound healing materials, 3D bioprinting and tissue engineering, and vaccines. Additionally, the biosensing applications of this key group of hydrogels will be discussed mainly focusing the attention on cancer detection.
Efficient Near-Field Radiofrequency Imaging of Impact Damage on CFRP Materials with Learning-Based Compressed Sensing
Authors: Song Huadong , Wang Zijun , Zeng Yanli , Guo Xiaoting , Tang Chaoqing
Published: August 2022

Carbon fiber-reinforced polymer (CFRP) is a widely-used composite material that is vulnerable to impact damage. Light impact damages destroy the inner structure but barely show obvious change on the surface. As a non-contact and high-resolution method to detect subsurface and inner defect, near-field radiofrequency imaging (NRI) suffers from high imaging times. Although some existing works use compressed sensing (CS) for a faster measurement, the corresponding CS reconstruction time remains high. This paper proposes a deep learning-based CS method for fast NRI, this plugin method decreases the measurement time by one order of magnitude without hardware modification and achieves real-time imaging during CS reconstruction. A special 0/1-Bernoulli measurement matrix is designed for sensor scanning firstly, and an interpretable neural network-based CS reconstruction method is proposed. Besides real-time reconstruction, the proposed learning-based reconstruction method can further reduce the required data thus reducing measurement time more than existing CS methods. Under the same imaging quality, experimental results in an NRI system show the proposed method is 20 times faster than traditional raster scan and existing CS reconstruction methods, and the required data is reduced by more than 90% than existing CS reconstruction methods.
Enhanced Energy Storage Performance in Na0.5Bi0.5TiO3-Based Relaxor Ferroelectric Ceramics via Compositional Tailoring
Authors: Jiang Yuleng , Niu Xiang , Liang Wei , Jian Xiaodong , Shi Hongwei , Li Feng , Zhang Yang , Wang Ting , Gong Weiping , Zhao Xiaobo , Yao Yingbang , Tao Tao , Liang Bo , Lu Shengguo
Published: August 2022

Owing to the high power density, excellent operational stability and fast charge/discharge rate, and environmental friendliness, the lead-free Na0.5Bi0.5TiO3 (NBT)-based relaxor ferroelectrics exhibit great potential in pulsed power capacitors. Herein, novel lead-free (1−x)(0.7Na0.5Bi0.5TiO3-0.3Sr0.7Bi0.2TiO3)-xBi(Mg0.5Zr0.5)O3 (NBT-SBT-xBMZ) relaxor ferroelectric ceramics were successfully fabricated using a solid-state reaction method and designed via compositional tailoring. The microstructure, dielectric properties, ferroelectric properties, and energy storage performance were investigated. The results indicate that appropriate Bi(Mg0.5Zr0.5)O3 content can effectively enhance the relaxor ferroelectric characteristics and improve the dielectric breakdown strength by forming fine grain sizes and diminishing oxygen vacancy concentrations. Therefore, the optimal Wrec of 6.75 J/cm3 and a η of 79.44% were simultaneously obtained in NBT-SBT-0.15BMZ at 20 °C and 385 kV/cm. Meanwhile, thermal stability (20–180 °C) and frequency stability (1–200 Hz) associated with the ultrafast discharge time of ~49.1 ns were also procured in the same composition, providing a promising material system for applications in power pulse devices.
BTO-Coupled CIGS Solar Cells with High Performances
Authors: Li Congmeng , Luo Haitian , Gu Hongwei , Li Hui
Published: August 2022

In order to improve the power conversion efficiency (PCE) of Cu(In,Ga)Se2 (CIGS) solar cells, a BaTiO3 (BTO) layer was inserted into the Cu(In,Ga)Se2. The performances of the BTO-coupled CIGS solar cells with structures of Mo/CIGS/CdS/i-ZnO/AZO, Mo/BTO/CIGS/CdS/i-ZnO/AZO, Mo/CIGS/BTO/CdS/i-ZnO/AZO, Mo/CIGS/CdS/BTO/i-ZnO/AZO, Mo/CIGS/BTO/i-ZnO/AZO, Mo/CIGS/CdS/BTO/AZO, and Mo/ CIGS/CdS(5 nm)/BTO(5 nm)/i-ZnO/AZO were systematically studied via the SCAPS-1D software. It was found that the power conversion efficiency (PCE) of a BTO-coupled CIGS solar cell with a device configuration of Mo/CIGS/CdS/BTO/AZO was 24.53%, and its open-circuit voltage was 931.70 mV. The working mechanism for the BTO-coupled CIGS solar cells with different device structures was proposed. Our results provide a novel strategy for improving the PCE of solar cells by combining a ferroelectric material into the p-n junction materials.
The Influence of Heat Treatment on Corrosion Resistance and Microhardness of Hot-Dip Zinc Coating Deposited on Steel Bolts
Authors: Jędrzejczyk Dariusz , Szatkowska Elżbieta
Published: August 2022

The analyzed topic refers to the corrosion resistance and changes in microhardness of the heat-treated (HT) hot-dip zinc coating deposited on bolts. The research aimed to evaluate the influence of the HT on the increase of the coating hardness and changes in anticorrosion properties. Hot-dip zinc coating was deposited in industrial conditions (acc. EN ISO 10684) on chosen bolts (M12x60). The achieved results were assessed based on corrosion resistance tests in neutral salt spray (salt chamber) and microhardness measurements. Tests were conducted in accordance with the adopted fractional plan, generated in the DOE module of the Statistica software. Using the conjugate gradient method optimal parameters of HT were determined. The conducted tests proved that the controlled heat treatment may increase the hardness of the hot-dip zinc coating without a significant deterioration in its basic protective function (corrosion resistance). The observed changes in the hardness and corrosion resistance of the zinc coating are a consequence of changes in its structure.
A Modified γ-Sutte Indicator for Air Quality Index Prediction
Authors: Shih Dong-Her , Hien To Thi , Nguyen Ly Sy Phu , Wu Ting-Wei , Lai Yen-Ting
Published: August 2022

Air pollution has become an essential issue in environmental protection. The Air Quality Index (AQI) is often used to determine the severity of air pollution. When the AQI reaches the red level, the proportion of asthma patients seeking medical treatment will increase by 30% more than usual. If the AQI can be predicted in advance, the benefits of early warning can be achieved. In recent years, a scholar has proposed an α-Sutte indicator which shows its excellence in time series prediction. However, the calculation of α-Sutte indicators uses a fixed weight. Thus, a β-Sutte indicator, using a dynamic weight with a high computation cost, has appeared. However, the computational complexity and sliding window required of the β-Sutte indicator are still high compared to the α-Sutte indicator. In this study, a modified γ-Sutte indicator, using a dynamic weight with a lower computational cost than the β-Sutte indicator, is proposed. In order to prove that the proposed γ-Sutte indicator has good generalization ability and is transferable, this study uses data from different regions and periods to predict the AQI. The results showed that the prediction accuracy of the γ-Sutte indicator proposed was better than other methods.
Identification of Continuous-Discrete Hidden Markov Models with Multiplicative Observation Noise
Authors: Borisov Andrey , Gorshenin Andrey
Published: August 2022

The paper aims to identify hidden Markov model parameters. The unobservable state represents a finite-state Markov jump process. The observations contain Wiener noise with state-dependent intensity. The identified parameters include the transition intensity matrix of the system state, conditional drift and diffusion coefficients in the observations. We propose an iterative identification algorithm based on the fixed-interval smoothing of the Markov state. Using the calculated state estimates, we restore all required system parameters. The paper contains a detailed description of the numerical schemes of state estimation and parameter identification. The comprehensive numerical study confirms the high precision of the proposed identification estimates.
Existence of Positive Solutions for a Fully Fourth-Order Boundary Value Problem
Authors: Li Yongxiang , Ma Weifeng
Published: August 2022

This paper deals with the existence of positive solutions of the fully fourth-order boundary value pqroblem u(4)=f(t,u,u′,u″,u‴) on [0,1] with the boundary condition u(0)=u(1)=u″(0)=u″(1)=0, which models a statically bending elastic beam whose two ends are simply supported, where f:[0,1]×R+×R×R−×R→R+ is continuous. Some precise inequality conditions on f guaranteeing the existence of positive solutions are presented. The inequality conditions allow that f(t,u,v,w,z) may be asymptotically linear, superlinear, or sublinear on u,v,w, and z as |(u,v,w,z)|→0 and |(u,v,w,z)|→∞. Our discussion is based on the fixed point index theory in cones.
Some Subclasses of Spirallike Multivalent Functions Associated with a Differential Operator
Authors: Ali Ekram Elsayed , Aouf Mohamed Kamal , El-Ashwah Rabha Mohamed , Bulboacă Teodor
Published: August 2022

In this paper we study convolution properties of spirallike multivalent functions defined by using a differential operator and higher order derivatives. Using convolution product relations we determine necessary and sufficient conditions for multivalent functions to belong to these classes, and our results generalized many previous results obtained by different authors. We obtain convolution and inclusion properties for new subclasses of multivalent functions defined by using the Dziok-Srivatava operator. Moreover, using a result connected with the Briot-Bouquet differential subordination, we obtain an inclusion relation between some of these classes of functions.
Maker Math: Exploring Mathematics through Digitally Fabricated Tools with K–12 In-Service Teachers
Authors: Harron Jason R. , Jin Yi , Hillen Amy , Mason Lindsey , Siegel Lauren
Published: August 2022

This paper reports on nine elementary, middle, and high school in-service teachers who participated in a series of workshops aimed at exploring the wonder, joy, and beauty of mathematics through the creation and application of digitally fabricated tools (i.e., laser-cut and 3D printed). Using the Technological Pedagogical and Content Knowledge (TPACK) framework to investigate technological, pedagogical, contextual, and content knowledge, researchers applied qualitative methods to uncover the affordances and constraints of teaching and learning math concepts with digitally fabricated tools and examined how the workshops supported broadening participation in mathematics by focusing on the connections between mathematical inquiry, nature, and the arts. Affordances include opportunities for hands-on learning, visual support at the secondary level, and real-world connections that go beyond the state standards. Barriers include purchasing a laser-cutter, ventilation and noise issues, time constraints, misalignment with school and district priorities, and a lack of administrative support. All participants indicated that they were interested in additional workshops focused on designing their own digitally fabricated mathematics tools that better align with their grade level(s) and standards.
Dynamic Analysis of a Delayed Carbon Emission-Absorption Model for China’s Urbanization and Population Growth
Authors: Sui Haokun , Han Leilei , Ding Yuting
Published: August 2022

The increase of carbon emissions accelerates global warming and causes environmental problems. Therefore, research on emission peak and carbon neutrality is of broad interest. In this paper, based on the relationships among carbon emissions, carbon absorption and urban population growth, a delayed differential equations model is established. Then, we analyze the existence and stability of a positive equilibrium point of the system and substitute the data for simulations. Eventually, according to theoretical analysis and numerical simulations, some suggestions for China to achieve the goal of emission peak and carbon neutrality are given.
General Anesthetics in Cancer Surgery: Can Anesthesiologists Help the Patient with More than a Safe Sleep
Authors: Bonvini John Michael
Published: August 2022

Most patients suffering from neoplastic diseases will at some point during their illness be approached surgically. Surgery itself may be unfortunately responsible for tumor proliferation and metastatic spread. With the perioperative period increasingly becoming a focus of research in anesthesia, anesthesiologists have looked at the chance to influence cancer progression based on their choice of anesthesia regimen and strategy. Many anesthetic agents have been investigated for their potential impact on the course of cancer disease. There is an abundance of retrospective studies and very few prospective ones that tackled this issue. The aim of this article is to review the current state of the evidence on general anesthesia involving volatile and intravenous agents as substrates, focusing on halogenated inhalational agents and propofol, to guide clinical decision making in assessments of the best practice for perioperative management of cancer surgery.
Combining Computational Screening and Machine Learning to Predict Metal–Organic Framework Adsorbents and Membranes for Removing CH4 or H2 from Air
Authors: Li Huilin , Wang Cuimiao , Zeng Yue , Li Dong , Yan Yaling , Zhu Xin , Qiao Zhiwei
Published: August 2022

Separating and capturing small amounts of CH4 or H2 from a mixture of gases, such as coal mine spent air, at a large scale remains a great challenge. We used large-scale computational screening and machine learning (ML) to simulate and explore the adsorption, diffusion, and permeation properties of 6013 computation-ready experimental metal–organic framework (MOF) adsorbents and MOF membranes (MOFMs) for capturing clean energy gases (CH4 and H2) in air. First, we modeled the relationships between the adsorption and the MOF membrane performance indicators and their characteristic descriptors. Among three ML algorithms, the random forest was found to have the best prediction efficiency for two systems (CH4/(O2 + N2) and H2/(O2 + N2)). Then, the algorithm was further applied to quantitatively analyze the relative importance values of seven MOF descriptors for five performance metrics of the two systems. Furthermore, the 20 best MOFs were also selected. Finally, the commonalities between the high-performance MOFs were analyzed, leading to three types of material design principles: tuned topology, alternative metal nodes, and organic linkers. As a result, this study provides microscopic insights into the capture of trace amounts of CH4 or H2 from air for applications involving coal mine spent air and hydrogen leakage.
Factors Affecting the Mechanical Performance of High Manganese Austenitic Steel
Authors: Xia Lei , Yan Ling , Zhang Hongmei , Li Yan , Jiang Zhengyi , Li Guanglong
Published: August 2022

High manganese austenitic steel has attracted increasing attention for its application in liquefied natural gas storage tank materials due to its excellent ductility and low cost. This paper presents an overview of the research progress of high manganese austenitic steel in recent years. As a structural material used at a low temperature environment, high manganese steel should not only have certain strength, but also good toughness to prevent brittle fracture at a low temperature. In this work, factors affecting mechanical properties of high manganese steel are discussed, possible reasons for the deterioration of low-temperature properties are analyzed, and the strengthening and toughening mechanisms of materials are elaborated, which may be beneficial to improve properties of high manganese austenitic steel.
High Prevalence and Varied Distribution of Antibiotic-Resistant Bacteria in the Rhizosphere and Rhizoplane of Citrus medica
Authors: Yang Fang , Wang Yu , Liu Qianwen , Xu Bo , Chen Huan , Li Yaomen , Wang Kun , Liang Guixin , Zhang Ruiqi , Jiao Xin’an , Zhang Yunzeng
Published: August 2022

The plant-associated bacteria, including that in the rhizosphere and rhizoplane, play important roles in human exposure to antibiotic-resistant bacteria (ARB). The rhizosphere and rhizoplane represent two distinct environments with different selective pressures for bacterial colonization. However, whether the difference in characteristics between the rhizosphere and rhizoplane can affect the abundance and antibiotic resistance profiles of ARB colonizing, the two environments remain largely unknown. In this study, we obtained 174 bacterial isolates from the rhizosphere (113 isolates) and rhizoplane (61 isolates) of Citrus medica trees grown in a park, where humans could easily and frequently contact the trees. A very high proportion of isolates exhibited resistance to several clinically important antibiotics, including β-lactam class antibiotics and polymyxin, with several known antibiotic-resistant opportunistic pathogens, such as Micrococcus luteus, being identified. The prevalence of ARB in the rhizoplane was higher than that in the rhizosphere. While the prevalence of polymyxin-resistant isolates was higher in the rhizoplane, the prevalence of amphenicol-resistant isolates was significantly higher in the rhizosphere. In summary, our findings suggest that the rhizosphere and rhizoplane are important media for the spread of ARB, and the different characteristics between the two environments can affect the distribution of ARB.
Degradation of Triclosan in the Water Environment by Microorganisms: A Review
Authors: Yin Yiran , Wu Hao , Jiang Zhenghai , Jiang Jingwei , Lu Zhenmei
Published: August 2022

Triclosan (TCS), a kind of pharmaceuticals and personal care products (PPCPs), is widely used and has had a large production over years. It is an emerging pollutant in the water environment that has attracted global attention due to its toxic effects on organisms and aquatic ecosystems, and its concentrations in the water environment are expected to increase since the COVID-19 pandemic outbreak. Some researchers found that microbial degradation of TCS is an environmentally sustainable technique that results in the mineralization of large amounts of organic pollutants without toxic by-products. In this review, we focus on the fate of TCS in the water environment, the diversity of TCS-degrading microorganisms, biodegradation pathways and molecular mechanisms, in order to provide a reference for the efficient degradation of TCS and other PPCPs by microorganisms.
Microbial Burden Estimation of Food Items, Built Environments, and the International Space Station Using Film Media
Authors: Simpson Anna C. , Suzuki Takeo , Miller Daniel R. , Venkateswaran Kasthuri
Published: August 2022

The use of film media involves considerably less preparation, waste, and incubator space than conventional agar-media-based assays and has proven in past studies to provide counts of cultivable microbes similar to those of traditional agar media. Film media also have the advantage of allowing sample volumes similar to those used in pour plates and, therefore, are well-suited for cultivable microbial counts in extremely low-biomass environments such as clean rooms or space habitats, particularly where the subsequent isolation of colonies is necessary. As the preparation of film media plates relies on water cohesion/adhesion rather than manual spreading, they may have future applications in low- or microgravity settings. In this study, cultivable microbial count performance was compared between agar media and film media in three kinds of samples: food items, surfaces in built environments on Earth (homes), and on the environmental surfaces of the International Space Station (ISS). Easy Plates (Kikkoman Corporation) and Petrifilm (3M) were compared with traditional agar plating for food and home surfaces, while only Easy Plates were compared with agar for ISS samples. For both food items and built environments on Earth, both types of film media performed comparably to agar media for bacterial counts, with R2 values of 0.94–0.96. Fungal counts for built-environment samples had a lower correlation between film and agar counts, with R2 values of 0.72–0.73. Samples from the ISS, which ranged from below detection to 103 CFU per 100 cm2, had R2 values of 0.80 for bacterial counts and 0.73 for fungal counts, partially due to multiple samples recording below the detection limit for agar or too numerous to count, and the growth of fungal species on R2A medium. The species compositions of isolates picked from agar vs. film media plates were similar; however, further phylogenetic analysis is needed to confirm the differential microbial diversity composition. Overall, film media such as Easy Plates and Petrifilm are viable alternatives to agar plates for low-biomass built environments as well as for food samples, and the two brands tested in this study performed equally well.