bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of Hierarchical Nickel-Cobalt Phosphide/Nickel Oxide with Tunable Electronic Structure and Strong Chemical Interface for Advanced Supercapacitors
Authors: Zhang Gaini , Liu Jingqian , Shan Hui , Ma Zhengdong , Xu Yuhui , Yang Zihao , Zuo Jiaxuan , Wang Jingjing , Li Shufeng , Li Xifei
Published: December 2023

The design of a reasonable heterostructure electrode to achieve enhanced areal performance for supercapacitors remains a great challenge. Here, we constructed hierarchical porous NiCoP/NiO nanocomposites anchored on Ni foam with tunable electronic and structural properties, as well as robust interfacial interaction. In NiCoP/NiO, the interconnected NiO nanosheets serve as a carrier with enriched anchoring sites to confine the NiCoP and improve its stability. Meanwhile, the ultrathin NiCoP nanosheets with bimetallic centers are connected with porous NiO nanosheets to form a reliable heterojunction, enhancing the electrochemical reaction kinetics. Taking advantage of the synergistic contribution of bimetallic centers, phosphides and unique structure, the NiCoP/NiO delivers a high areal specific capacitance (1860 mF cm−2 at 5 mA cm−2), good rate performance of 78.5% at six times the increased current density, and remarkable durability (11.0% decrease after 10,000 cycles). Furthermore, the assembled hybrid supercapacitor NiCoP/NiO//porous-activated carbon (PAC) delivers a high areal energy density of 173.7 μWh cm−2 (116.4 μWh cm−2) at 1.6 mW cm−2 (32 mW cm−2). The results indicate that the design of the heterostructure interface with strong chemical interface and tunable electronic structure is an effective and promising approach to boost the electrochemical performance for advanced supercapacitors.
Differential Regulation of Intracisternally Injected Angiotensin II-Induced Mechanical Allodynia and Thermal Hyperalgesia in Rats
Authors: Park Ki-Don , Son Jo-Young , Kim Hak-Kyun , Kim Yu-Mi , Ju Jin-Sook , Jo Min-Jeong , Park Min-Kyoung , Lee Min-Kyung , Ahn Dong-Kuk
Published: December 2023

The present study examined the underlying mechanisms of mechanical allodynia and thermal hyperalgesia induced by the intracisternal injection of angiotensin (Ang) II. Intracisternal Ang II injection decreased the air puff threshold and head withdrawal latency. To determine the operative receptors for each distinct type of pain behavior, we intracisternally injected Ang II receptor antagonists 2 h after Ang II injection. Losartan, an Ang II type 1 receptor (AT1R) antagonist, alleviated mechanical allodynia. Conversely, PD123319, an Ang II type 1 receptor (AT2R) antagonist, blocked only thermal hyperalgesia. Immunofluorescence analyses revealed the co-localization of AT1R with the astrocyte marker GFAP in the trigeminal subnucleus caudalis and co-localization of AT2R with CGRP-positive neurons in the trigeminal ganglion. Intracisternal pretreatment with minocycline, a microglial inhibitor, did not affect Ang II-induced mechanical allodynia, whereas L-α-aminoadipate, an astrocyte inhibitor, significantly inhibited Ang II-induced mechanical allodynia. Furthermore, subcutaneous pretreatment with botulinum toxin type A significantly alleviated Ang II-induced thermal hyperalgesia, but not Ang II-induced mechanical allodynia. These results indicate that central Ang II-induced nociception is differentially regulated by AT1R and AT2R. Thus, distinct therapeutic targets must be regulated to overcome pain symptoms caused by multiple underlying mechanisms.
Genomic Landscape Comparison of Cardiac versus Extra-Cardiac Angiosarcomas
Authors: Gozzellino Livia , Nannini Margherita , Urbini Milena , Pizzi Carmine , Leone Ornella , Corti Barbara , Baldovini Chiara , Angeli Francesco , Foà Alberto , Pacini Davide , Folesani Gianluca , Costa Alice , Palumbo Teresa , Nigro Maria Concetta , Pasquinelli Gianandrea , Astolfi Annalisa , Pantaleo Maria Abbondanza
Published: December 2023

Angiosarcomas (ASs) are rare malignant vascular entities that can affect several regions in our body, including the heart. Cardiac ASs comprise 25–40% of cardiac sarcomas and can cause death within months of diagnosis. Thus, our aim was to identify potential differences and/or similarities between cardiac and extra-cardiac ASs to enhance targeted therapies and, consequently, patients’ prognosis. Whole-transcriptome analysis of three cardiac and eleven extra-cardiac non-cutaneous samples was performed to investigate differential gene expression and mutational events between the two groups. The gene signature of cardiac and extra-cardiac non-cutaneous ASs was also compared to that of cutaneous angiosarcomas (n = 9). H/N/K-RAS and TP53 alterations were more recurrent in extra-cardiac ASs, while POTE-gene family overexpression was peculiar to cardiac ASs. Additionally, in vitro functional analyses showed that POTEH upregulation conferred a growth advantage to recipient cells, partly supporting the cardiac AS aggressive phenotype and patients’ scarce survival rate. These features should be considered when investigating alternative treatments.
Foot Bionics Research Based on Reindeer Hoof Attachment Mechanism and Macro/Microstructures
Authors: Li Guoyu , Zhang Rui , Luo Yexuan , Liu Yue , Cao Qiang , Song Jiafeng
Published: December 2023

The attachment performances of mechanical feet are significant in improving the trafficability and mobility of robots on the extreme ground. In the future, frozen-ground robots can be used to replace human soldiers in scouting and deep space exploration. In this study, the influence factors on the attachment function of the bionic feet were analyzed. Soft frozen soil and tight frozen soil close to natural frozen soil were prepared, and the friction between ungula and frozen soil ground was simulated together with the plantar pressures of reindeer under trotting. The major attachment parts were the ungula cusp, outer edges, and ungula capsules, and the stress on the ungula was mainly 4.56–24.72 MPa. According to the microstructures of plantar fur and ungula, the corresponding ratio of the rib width and length was 0.65:1, and the corresponding ratio of the rib width and distance was 3:1. In addition, the scales of the plantar fur were very tightly arranged and had large ripples. Based on typical curves, an ungula capsule-curved surface, and a nonsmooth plantar fur surface, four types of bionic feet and the corresponding ordinary multidamboard foot were designed. On the frozen soil, the bionic foot with ribs and an ungula capsule showed the best attachment performance. Compared with the multidamboard foot, the dynamic coefficient of friction of the bionic foot with ribs and ungula capsules increased by 11.43–31.75%. The attachment mechanism of the bionic feet is as follows: under the action of pressure, the fine patterns of the bionic convex-crown generate friction with the nonsmooth structure of the frozen soil surface, which improves the attachment performance.
Optimizing the Fly Ash/Activator Ratio for a Fly Ash-Based Geopolymer through a Study of Microstructure, Thermal Stability, and Electrical Properties
Authors: Yadav Meenakshi , Kumar Lalit , Yadav Vikas , Jagannathan Karthikeyan , Singh Vidya Nand , Singh Surinder P. , Ezhilselvi V.
Published: December 2023

Fly ash (FA)-based geopolymer was prepared using sodium hydroxide and sodium silicate (in 2.5ratio) as an alkali activator liquid (AL). The condition of FA/AL was optimized for achieving 1.00, 1.25, 1.5, and 1.75 ratios by varying the alkali concentrations, which are referred to as GP1, GP2, GP3, and GP4, respectively. The influence of slight variations in the FA/AL ratio on microstructure, morphology, functional groups, and composition was investigated by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), X-ray fluorescence (XRF), and Fourier transform infrared spectroscopy (FTIR). FESEM detected a homogeneous fused matrix of fly ash and alkali activator solution up to 1.5 ratios; GP3 showed a dense morphology. FTIR confirmed that the formation of aluminosilicate gel induced a shift in the T–O (T = Si or Al) asymmetric stretching band, nearing a lower frequency. XRD showed an amorphous structure with phases, including quartz, mullite, hematite, and sodalite. The thermogravimetry and differential thermal analysis (TGA–DTA) indicated that the geopolymer samples were thermally stable. The electrical study concluded that the geopolymer possessed insulating properties.
Structural Characterization, Antioxidant, and Antiviral Activity of Sulfated Polysaccharide (Fucoidan) from Sargassum asperifolium (Turner) J. Agardh
Authors: Ageeli Abeer A. , Mohamed Sahera Fathalla
Published: December 2023

Brown algae possess a diverse array of acidic polysaccharides, including fucoidan. The present research intends to investigate the extraction and characterization of algal polysaccharides to explore their antiviral activity. A light brown sulfated polysaccharide was extracted (with a yield of 18% of dry weight) from Sargassum asperifolium algal powder. The results of fractionation of sulfated polysaccharide revealed the occurrence of two primary fractions: low-sulfated polysaccharides (SPF1) and high-sulfated polysaccharides (SPF2). The bioassays conducted on SPF2 demonstrated a greater level of antioxidant activity compared to SPF1, with respective IC50 values of 17 ± 1.3 µg/mL and 31 ± 1.1 μg/mL after a duration of 120 min. The cytotoxicity of SPF2 on Vero cells was determined, and the calculated half-maximal cytotoxic concentration (CC50) was found to be 178 ± 1.05 µg/mL. Based on these results, an antiviral activity assay was conducted on SPF2. The results demonstrated that SPF2 had greater efficacy against Hepatitis A Virus (HAV) compared to Herpes Simplex Virus Type 1 (HSV-1), with corresponding half-maximal inhibitory concentrations (IC50) of 48 ± 1.8 µg/mL and 123 ± 2.6 µg/mL, respectively. The active SPF2 was characterized by FT-IR, 1H, and 13C NMR spectroscopy. The extracted fucoidan can be used as a natural therapeutic agent in combating various viral infections.
Pain in Adolescence: Maternal and Paternal Factors Affecting Adolescents’ Pain in Norway—A Cross-Sectional Study
Authors: Grasaas Erik , Mikkelsen Hilde Timenes , Haraldstad Kristin , Helseth Sølvi , Småstuen Milada Cvancarova , Skarstein Siv , Rohde Gudrun Elin
Published: December 2023

Background: Pain in adolescence is considered a worldwide concern. Adolescents’ pain affects family functioning. However, bidirectional associations should be considered as parental determinates such as pain, stress, and sociodemographic factors are also shown to influence pain in adolescence. Objectives: This study explored the associations between maternal and paternal sociodemographic factors, pain, and stress and adolescents’ pain, and stress on adolescents’ pain. Methods: In total, 508 school-based Norwegian adolescents with a corresponding parent were included. All adolescents completed an electronic survey during school hours, and their respective parents responded electronically. The survey included sociodemographic data, the Perceived Stress Questionnaire, and the Brief Pain Inventory. Results: Herein, 385 adolescents reported an average pain of 2.1 (SD, 1.9), and 308 of the participating parents reported an average pain of 1.6 (SD, 1.8). Regressions stratified by parental gender revealed nonsignificant associations in fathers’ study variables predicting adolescents’ pain. However, having the highest maternal educational level (p ≤ 0.01) and working part-time (p ≤ 0.01) were associated with lower pain in adolescents. Conclusions: The findings of this study demonstrated that sociodemographic factors such as high educational status in mothers and mothers working part-time were associated with lower pain in Norwegian adolescents. These findings highlight the importance of a holistic approach to pain management in adolescence.
Urine Sampling Protocol Recommendations for Reliable Determination of Total Urinary Luteinizing Hormone Immunoreactivity in the Pediatric Population
Authors: Demir And , Aydin Adem , Büyükgebiz Atilla
Published: December 2023

This study investigates day-to-day variations in urinary luteinizing hormone (U-LH) concentrations in children, focusing on potential minimization or correction methods. 95 children and adolescents (51 boys, 44 girls, ages 5–17) provided daytime and evening urine samples for U-LH determinations over three consecutive days. No consistent day-to-day differences in U-LH levels were observed, although random variations, particularly in adolescents aged 13 or older, were noted. The net inter-assay CV% for U-LH changes over three days showed high variability, averaging 24.6% to 28.0% for boys and 21.6% to 27.3% for girls, independent of sex, collection time, or U-LH level. To reliably determine total urinary luteinizing hormone immunoreactivity in the pediatric population, it is advisable to collect multiple first-morning voided samples for at least three consecutive days as an interim solution, pending the development of a standardized protocol or correction method for varying urine composition. Strict adherence, especially for adolescents aged 13 or older, is vital.
Indications from C:N:P Ratios in Surface Sediments along Land-to-Sea Gradients to Support Coastal Nutrient Management
Authors: Karstens Svenja , Friedrich Jenny , Geuer Jana K. , Grosser Katharina , Schneider von Deimling Jens
Published: December 2023

Shallow, semi-enclosed coastal systems are particularly prone to eutrophication. Depending on local site conditions and historical nutrient legacies, sea-based measures might be necessary in addition to land-based nutrient removal. In this study, C:N:P ratios were combined with open-source bathymetric information and linked with the prevailing geomorphological and sedimentological regimes to gain insights into nutrient hotspots and understand their sources and fate in coastal waters. Land-based sediment samples were taken behind outlets at three sites in Eckernförde Bay (Baltic Sea), and complemented with ship-based sampling at locations approximately 8 m and 12 m water depth. The total carbon, nitrogen and phosphorus concentrations in surface sediments increased at deeper sites. This suggests that an increased downslope particle transport and deposition regime, based on local geomorphology, might influence nutrient hotspots to a larger extent than proximity to sources (e.g., outlets). Overall, the recorded C:N ratios (mean = 28.12) were closer to the ratio of terrestrial plants than those of marine phytoplankton, indicating allochthonous sources of organic matter.
Anastomosis Complications after Bronchoplasty: Incidence, Risk Factors, and Treatment Options Reported by a Referral Cancer Center
Authors: Girelli Lara , Bertolaccini Luca , Casiraghi Monica , Petrella Francesco , Galetta Domenico , Mazzella Antonio , Donghi Stefano , Lo Iacono Giorgio , Cara Andrea , Guarize Juliana , Spaggiari Lorenzo
Published: December 2023

Background: Sleeve lobectomy with bronchoplasty is a safe surgical technique for the management of lung cancer and endobronchial localization of extrapulmonary cancers. However, anastomotic complications can occur, and treatment strategies are not standardized. Methods: Data from 280 patients subjected to bronchoplasty were retrospectively analyzed, focusing on surgical techniques, anastomotic complications, and their management. Multivariate analysis was performed, and Kaplan–Meier curves were used to determine survival. Results: Ninety percent of 280 surgeries were for lung cancer. Anastomotic complications occurred in 6.42% of patients: late stenosis in 3.92% and broncho-pleural fistula in 1.78%. The median survival was 65.90 months (95% CI = 41.76–90.97), with no difference (p = 0.375) for patients with (51.28 months) or without (71.03 months) anastomotic complications. Mortality at 30 days was higher with anastomotic complications (16.7% vs. 3%, p = 0.014). Multivariable analysis confirmed pathological stage (N+) as a risk factor for anastomotic complications (p = 0.016). Our mortality (3.93%) and morbidity rate (41.78%) corresponded to recent series results. Conclusions: In our experience, surgery is preferred to avoid life-threatening complications in bronchopleural fistulas. Bronchoscopic balloon dilatation is preferred for benign strictures. The nodal stage is related to complications (p = 0.0014), reflecting the aggressiveness of surgery, which requires extended radical lymphadenectomy.
A Compact Slotted UWB Antenna Based on Characteristics Mode Theory for Wireless Applications
Authors: Kempanna Subhash Bodaguru , Biradar Rajashekhar C. , Ali Tanweer , Jhunjhunwala Vikash Kumar , Soman Sarun , Pathan Sameena
Published: December 2023

The development of electronic systems and wireless communication has led to a proportional increase in data traffic over time. One potential solution for alleviating data congestion is to augment the bandwidth capacity. This study presents a novel asymmetric circular slotted semi-circle-shaped monopole antenna design using a defective ground structure. The extended ultrawide bandwidth is achieved by implementing a design where the semi-circle radiator is etched in a specific asymmetric circular slot. This involves etching a circle with a radius of 1.25 mm at the center of the radiator, as well as a succession of circles with a radius of 0.75 mm along the edges of the radiator. In addition, the ground plane is situated at a lower elevation and features a U-shaped truncation that has been etched onto its surface. The expansion of the impedance bandwidth can be accomplished by making adjustments to the radiator and ground plane. The UWB antenna under consideration possesses a geometric configuration of 21.6 × 20.8 × 1.6 mm3 and the antenna is fabricated using an FR-4 glass epoxy substrate. The UWB antenna operates throughout the frequency range of 2.2–16.5 GHz, exhibiting a gain of at least 3.45 dBi across the entire impedance bandwidth and the maximum peak gain of 9.57 dBi achieved at the mid-resonance frequency of 10.5 GHz. The investigation of the antenna’s physical properties is conducted utilizing characteristic mode analysis. The investigation also includes an analysis of the time-domain characteristics, revealing that the group delay was found to be less than 1 ns across the operational frequency range. The predicted and measured findings demonstrate consistency and confirm that the suggested antenna is suitable for electronic systems and wireless applications.
Effect of Commercial Children’s Mouthrinses and Toothpastes on the Viability of Neonatal Human Melanocytes: An In Vitro Study
Authors: Goenka Shilpi , Lee Hsi-Ming
Published: December 2023

In this study, we examined the cytotoxic effects of six commercial children’s mouthrinses (designated as #1, #2, #3, #4, #5, and #6) and four commercial children’s toothpastes (designated as #1, #2, #3, and #4) on primary human neonatal melanocytes that were used as a representative model for oral melanocytes. Mouthrinses diluted directly with culture medium (1:2, 1:5, 1:10, 1:100, and 1:1000) were added to monolayers of melanocytes for 2 min, followed by 24 h recovery, after which MTS cytotoxicity assay was conducted. The extracts of each toothpaste were prepared (50% w/v), diluted in culture medium (1:2, 1:5, 1:10, 1:50, 1:100, and 1:1000), and added to cell monolayers for 2 min (standard brushing time), followed by an analysis of cell viability after 24 h. Results showed that all mouthrinses except mouthrinse #4 showed significantly greater loss of cell viability, ascribed to cetylpyridinium chloride (CPC) that induced significant cytotoxicity to melanocytes (IC50 = 54.33 µM). In the case of toothpastes, the examination of cellular morphology showed that a 2 min exposure to all toothpaste extracts induced a concentration-dependent decline in cell viability, pronounced in toothpaste containing sodium lauryl sulfate (SLS) detergent. Further results suggested SLS to be the critical driver of cytotoxicity (IC50 = 317.73 µM). It is noteworthy that toothpaste #1 exhibited much lower levels of cytotoxicity compared to the other three toothpastes containing SLS. Taken together, these findings suggest that the melanocytotoxicity of children’s mouthrinse (#4) and toothpaste (#1) is comparatively low. To the best of our knowledge, this is the first study to examine the impact of children’s toothpastes and mouthrinses on neonatal primary human melanocytes. Future studies to investigate these findings in a realistic scenario replicating oral cavity conditions of the presence of microbiota, pellicle layer and saliva, and other cell types are warranted.
Results for Nonlinear Diffusion Equations with Stochastic Resetting
Authors: Lenzi Ervin K. , Zola Rafael S. , Rosseto Michely P. , Mendes Renio S. , Ribeiro Haroldo V. , Silva Luciano R. da , Evangelista Luiz R.
Published: December 2023

In this study, we investigate a nonlinear diffusion process in which particles stochastically reset to their initial positions at a constant rate. The nonlinear diffusion process is modeled using the porous media equation and its extensions, which are nonlinear diffusion equations. We use analytical and numerical calculations to obtain and interpret the probability distribution of the position of the particles and the mean square displacement. These results are further compared and shown to agree with the results of numerical simulations. Our findings show that a system of this kind exhibits non-Gaussian distributions, transient anomalous diffusion (subdiffusion and superdiffusion), and stationary states that simultaneously depend on the nonlinearity and resetting rate.
Energy-Efficient and Timeliness-Aware Continual Learning Management System
Authors: Kang Dong-Ki
Published: December 2023

Continual learning has recently become a primary paradigm for deep neural network models in modern artificial intelligence services, where streaming data patterns frequently and irregularly change over time in dynamic environments. Unfortunately, there is still a lack of studies on computing cluster management for the processing of continual learning tasks, particularly in terms of the timeliness of model updates and associated energy consumption. In this paper, we propose a novel timeliness-aware continual learning management (TA-CLM) system aimed at ensuring timely deep neural network model updates for continual learning tasks while minimizing the energy consumption of computing worker nodes in clusters. We introduce novel penalty cost functions to penalize quantitatively deep neural network model update latency and present the associated optimization formulation to ensure the best task allocation. Additionally, we design a simulated annealing-based optimizer, which is a meta-heuristic technique and easy to implement, to solve the non-convex and non-linear optimization problem. We demonstrate that the proposed TA-CLM system improves both latency and energy performance over its competitors by an average of 51.3% and 51.6%, respectively, based on experimental results using raw data from well-known deep neural network models on an NVIDIA GPU-based testbed and a large-scale simulation environment.
Renewable Energy and Energy Efficiency: European Regional Policy and the Role of Financial Instruments
Authors: Batóg Jacek , Pluskota Przemysław
Published: December 2023

The study aimed to evaluate whether the regional funds allocated for energy efficiency and renewable energy are related to the quantity of air pollutants discharged and the stage of regional development, and whether the evidence of convergence of regional levels of renewable energy electricity generation can be provided. A comparative analysis of financial instrument implementation within regional programmes was conducted, with a particular focus on instruments dedicated to enhancing energy efficiency and utilising renewable energy. To verify the research hypotheses, statistical coefficients of correlation and concentration, along with trend and econometric models were applied. The findings have confirmed the rise in regional funds for energy efficiency and renewable energy, along with the growing importance of financial instruments in transforming the energy sector. The hypotheses that air pollutant emissions per unit of GDP generated are decreasing, there exists a regional convergence of renewable energy production per capita, and the spatial accumulation of renewable energy production is declining, have been confirmed. No correlation was found between the regional economic development and the level of funds allocated to energy efficiency and renewable energy. The lack of such relationships provides a convincing argument for appropriate state regulation.
The Application of Adaptive Model Predictive Control for Fed-Batch Escherichia coli BL21 (DE3) Cultivation and Biosynthesis of Recombinant Proteins
Authors: Dubencovs Konstantins , Suleiko Arturs , Sile Elina , Petrovskis Ivars , Akopjana Inara , Suleiko Anastasija , Galvanauskas Vytautas , Tars Kaspars , Vanags Juris
Published: December 2023

A model predictive control (MPC) method was investigated as a route to optimize and control the growth of E. coli BL21 (DE3) and biosynthesis of two different recombinant proteins (nerve growth factor NGF and coat protein of bacteriophage Qβ (Qβ-CP)). To determine the target trajectory for the E. coli cultivation process and estimate the model parameters, the off-line run-to-run optimization method was used. The proven method allowed us to successfully control the growth of microbial biomass, with a deviation of 6–12% from the target trajectory. It was proven that it is possible to obtain a “Golden Batch” profile for the implementation of MPC using datasets from only four to eight fermentation runs. The method showed its robustness when the cultivation of E. coli was carried out with two different titrant supply control systems—volumetric and gravimetric. Furthermore, the MPC method exhibited high adaptability, reliability, and resistance to various types of disturbances. MPC proved to be a reliable and effective method for controlling the cultivation and recombinant protein biosynthesis of fast-growing microorganisms such as E. coli.
Demonstrating the Kelvin-Helmholtz Instability Using a Low-Cost Experimental Apparatus and Computational Fluid Dynamics Simulations
Authors: Gibbons Melissa M. , Muldoon Dillon , Khalil Imane
Published: December 2023

A Kelvin-Helmholtz instability is formed when two fluids of different densities exert a shear on one another at their interface when flowing in opposite directions. This paper presents a step-by-step guide for the design of a low-cost, small-scale, experimental tilt tube apparatus and a corresponding computational fluid dynamics (CFD) model that can be used to introduce the Kelvin-Helmholtz instability to undergraduate mechanical engineering students in several courses. A thermal-fluids laboratory course is taken by our fourth-year mechanical engineering students, and the overall variety of experiments has been limited by the cost of commercial teaching equipment. The tilt tube apparatus allows students to induce and record the Kelvin-Helmholtz instability, and no ongoing costs are involved in incorporating this experiment into the course. In our introductory CFD course, students perform CFD simulations as part of the design and analysis process. Developing a two-dimensional (2D) CFD model with two different fluids is well within their capabilities after completing initial software and simulation tutorial exercises and homework. Representative experiments were conducted with fresh water and salt water of different densities, and results showed that both the amplitude of the waves and the amount of time the instability was visible decreased with increasing salt water salinity. Results from a 2D CFD model developed in Ansys Fluent exhibited the same trends as the experimental data.
Do Patients with Chronic Spinal Pain and Comorbid Insomnia Have More Features of Central Sensitization? A Case-Control Study
Authors: Araújo Almeida Lucas , Bilterys Thomas , Van Looveren Eveline , Mairesse Olivier , Cagnie Barbara , Meeus Mira , Moens Maarten , Goubert Dorien , Munneke Wouter , Danneels Lieven , Ickmans Kelly , Rezende Camargo Paula , Nijs Jo , Malfliet Anneleen , De Baets Liesbet
Published: December 2023

Background: Chronic spinal pain (CSP) is a major public health problem worldwide, frequently related to sleep problems. Central sensitization (CS) may worsen the clinical picture of CSP patients with insomnia. The aim of this study was to compare self-reported and objectively measured clinical outcomes between insomniac CSP patients with comorbid insomnia with and without symptoms of CS. Methods: A case-control study on baseline self-reported sleep, functioning, and psychological distress through online questionnaires. Objective sleep and physical activity parameters and pressure pain thresholds (PPTs) were assessed through polysomnography, actigraphy, and digital algometry, respectively. Independent sample t-test and Mann–Whitney U tests were used to examine possible differences in the outcome measures between the groups. Results: Data from 123 participants were included and revealed no statistically significant group for objective sleep and physical activity parameters. The CS group, however, presented with worse self-reported sleep (quality sleep, insomnia severity, and dysfunctional beliefs about sleep), increased mental and physical fatigue, and higher psychological distress (anxiety and depressive symptoms), and reported lower PPTs. Conclusions: symptoms of CS may influence perceived sleep and affect functional health and well-being perception but do not seem to affect objective sleep and physical activity.
Epinephrine Stimulates Mycobacterium tuberculosis Growth and Biofilm Formation
Authors: Lei Yingying , Rahman Khaista , Cao Xiaojian , Yang Bing , Zhou Wei , Reheman Aikebaier , Cai Luxia , Wang Yifan , Tyagi Rohit , Wang Zhe , Chen Xi , Cao Gang
Published: December 2023

The human stress hormones catecholamines play a critical role in communication between human microbiota and their hosts and influence the outcomes of bacterial infections. However, it is unclear how M. tuberculosis senses and responds to certain types of human stress hormones. In this study, we screened several human catecholamine stress hormones (epinephrine, norepinephrine, and dopamine) for their effects on Mycobacterium growth. Our results showed that epinephrine significantly stimulated the growth of M. tuberculosis in the serum-based medium as well as macrophages. In silico analysis and molecular docking suggested that the extra-cytoplasmic domain of the MprB might be the putative adrenergic sensor. Furthermore, we showed that epinephrine significantly enhances M. tuberculosis biofilm formation, which has distinct texture composition, antibiotic resistance, and stress tolerance. Together, our data revealed the effect and mechanism of epinephrine on the growth and biofilm formation of M. tuberculosis, which contributes to the understanding of the environmental perception and antibiotic resistance of M. tuberculosis and provides important clues for the understanding of bacterial pathogenesis and the development of novel antibacterial therapeutics.
Myeloproliferative Neoplasms: Contemporary Review and Molecular Landscape
Authors: Mahmud Muftah , Vasireddy Swati , Gowin Krisstina , Amaraneni Akshay
Published: December 2023

Myelofibrosis (MF), Myeloproliferative neoplasms (MPNs), and MDS/MPN overlap syndromes have a broad range of clinical presentations and molecular abnormalities, making their diagnosis and classification complex. This paper reviews molecular aberration, epigenetic modifications, chromosomal anomalies, and their interactions with cellular and other immune mechanisms in the manifestations of these disease spectra, clinical features, classification, and treatment modalities. The advent of new-generation sequencing has broadened the understanding of the genetic factors involved. However, while great strides have been made in the pharmacological treatment of these diseases, treatment of advanced disease remains hematopoietic stem cell transplant.
Childhood Obesity: Insight into Kidney Involvement
Authors: Carullo Nazareno , Zicarelli Mariateresa , Michael Ashour , Faga Teresa , Battaglia Yuri , Pisani Antonio , Perticone Maria , Costa Davide , Ielapi Nicola , Coppolino Giuseppe , Bolignano Davide , Serra Raffaele , Andreucci Michele
Published: December 2023

This review examines the impact of childhood obesity on the kidney from an epidemiological, pathogenetic, clinical, and pathological perspective, with the aim of providing pediatricians and nephrologists with the most current data on this topic. The prevalence of childhood obesity and chronic kidney disease (CKD) is steadily increasing worldwide, reaching epidemic proportions. While the impact of obesity in children with CKD is less pronounced than in adults, recent studies suggest a similar trend in the child population. This is likely due to the significant association between obesity and the two leading causes of end-stage renal disease (ESRD): diabetes mellitus (DM) and hypertension. Obesity is a complex, systemic disease that reflects interactions between environmental and genetic factors. A key mechanism of kidney damage is related to metabolic syndrome and insulin resistance. Therefore, we can speculate about an adipose tissue–kidney axis in which neurohormonal and immunological mechanisms exacerbate complications resulting from obesity. Adipose tissue, now recognized as an endocrine organ, secretes cytokines called adipokines that may induce adaptive or maladaptive responses in renal cells, leading to kidney fibrosis. The impact of obesity on kidney transplant-related outcomes for both donors and recipients is also significant, making stringent preventive measures critical in the pre- and post-transplant phases. The challenge lies in identifying renal involvement as early as possible, as it is often completely asymptomatic and not detectable through common markers of kidney function. Ongoing research into innovative technologies, such as proteomics and metabolomics, aims to identify new biomarkers and is constantly evolving. Many aspects of pediatric disease progression in the population of children with obesity still require clarification. However, the latest scientific evidence in the field of nephrology offers glimpses into various new perspectives, such as genetic factors, comorbidities, and novel biomarkers. Investigating these aspects early could potentially improve the prognosis of these young patients through new diagnostic and therapeutic strategies. Hence, the aim of this review is to provide a comprehensive exploration of the pathogenetic mechanisms and prevalent pathological patterns of kidney damage observed in children with obesity.
A Surface-Enhanced Raman Spectroscopic Sensor Pen
Authors: Song Zejiang , Li Zhijie , Zhan Weishen , Zhao Wanli , Chui Hsiang-Chen , Li Rui
Published: December 2023

Surface-enhanced Raman spectroscopy (SERS) is widely used as a detection method in scientific research fields. However, the method for creating SERS substrates often requires expensive equipment and involves a complex process. Additionally, preserving and effectively utilizing SERS substrates in the long term poses a challenging problem. In order to address these issues, we propose a new method for creating SERS substrates on various types of paper using a combination of a ballpoint pen and 3D printing. This method ensures a high enhancement factor and maximizes the utilization of the substrate. We achieved an enhancement factor of up to 8.2 × 108 for detecting R6G molecules, with a relative standard deviation of 11.13% for the Raman peak at 612 cm−1 of R6G, demonstrating excellent SERS sensitivity and spectral reproducibility. Furthermore, we successfully detected thiram at a concentration as low as 10−8, which is lower than both the Chinese national standard and European standard.
Navigating the Unexpected: Iatrogenic Aortic Injuries during Transcatheter Aortic Valve Replacement (TAVR)
Authors: Cavanaugh Shaelyn , Amirjamshidi Hossein , Hisamoto Kazuhiro
Published: December 2023

The introduction of transcatheter aortic valve replacement (TAVR) has undeniably changed the landscape of valvular heart disease management over the last two decades. A reduction in complications through improvements in techniques, experience, and technology has established TAVR as a safe and effective alternative to surgical aortic valve replacement. However, it is important to consider the potential risks associated with TAVR and ways in which life-threatening complications can be identified and managed in a timely fashion. In this article, we review some catastrophic iatrogenic aortic injuries that are described in the literature and present a case of an acute iatrogenic type A aortic dissection that occurred during a transcatheter aortic valve replacement (TAVR). After valve deployment, a routine neurologic examination noted the new onset of a left-sided facial droop and upper extremity weakness. Urgent imaging revealed an extensive type A aortic dissection, and the patient was taken to the operating room for surgical repair. The coordination of our multidisciplinary team allowed for prompt recognition of her neurologic symptoms, urgent imaging, and timely transport to the operating room, all of which contributed to the successful management of this life-threatening procedural complication.
The First Record of Ocypode sinensis (Decapoda: Ocypodidae) from the Korean Peninsula: How the Complete Mitochondrial Genome Elucidates the Divergence History of Ghost Crabs
Authors: Kim Da-In , Jang Sook-Jin , Kim Taewon
Published: December 2023

Ghost crabs, as a species of the Ocypode within the subfamily Ocypodinae, are distributed in the upper intertidal zone worldwide and are ecologically remarkable. They play an important role in the energy circulation in the intertidal zone and are used as an ecological indicator to predict the impacts of environmental change or anthropogenic activities on the marine ecosystem. In this study, we provide the first evidence for the distribution of O. sinensis in Jeju Island and the southern coastal area on the Korean Peninsula. We generated a high-fidelity mitochondrial genome (mitogenome) for the species. The mitogenome was assembled into a circular chromosome of 15,589 bp, including 13 protein-coding genes, two ribosomal RNA genes, and twenty-two transfer RNA genes. High genetic variation compared with closely related species enabled the precise reconstruction of phylogenetic relationships and an estimation of the divergence times among the Ocypode species. The phylogenetic inference indicated that O. sinensis forms a monophyletic clade with O. cordimanus and diverged from ancestral species approximately 20.41 million years ago.
Financial Intermediation, Economic Growth, and Business Cycles
Authors: Konstantakopoulou Ioanna
Published: December 2023

This paper aims to examine the importance of financial intermediation in economic activity. We also explore the effects of monetary factors and financial frictions on the relationship between financial intermediation and economic growth, the drivers of business cycles, and how shocks spread through the intermediation process. Financial intermediaries improve fund allocation, minimize monitoring costs, minimize liquidity risk, simplify risk management, and facilitate portfolio diversification and resource allocation to more productive activities. In addition, financial intermediaries collect and analyze information about investment projects, allocating resources and managing information more efficiently than individual investors. We conclude that financial intermediation is significant for economic growth. In addition, we show that financial market frictions can amplify exogenous shocks, affecting investment, economic growth rates, and macroeconomic stability. Reducing financial frictions through intermediation is crucial.
Industry 4.0 Implementation in Small- and Medium-Sized Enterprises: Recommendations Extracted from a Systematic Literature Review with a Focus on Maturity Models
Authors: Häring Kim , Pimentel Carina , Teixeira Leonor
Published: December 2023

Background: Industry 4.0 signifies a profound global transformation in production and service activities through its novel organizational principles, including digital connectivity, information transparency, technical assistance, and decentralized decision making. This transformation poses significant challenges for businesses, particularly small- and medium-sized enterprises (SMEs). In response, maturity models have been developed and adapted to facilitate a transparent and supportive entry into this transformative domain. Method/Aim: This study is dedicated to the comprehensive analysis of Industry 4.0 maturity models through a systematic literature review to identify and evaluate previously published recommendations for the adoption and utilization of Industry 4.0. The aim is to provide valuable insights in this context, with a particular focus on easing entry into this domain for SMEs. Results: Quantitative findings reveal a growing demand for fundamental support when entering this domain, with maturity models capable of meeting the demand for structured guidance. However, these models are currently under-validated, lacking transparency, and are often unsuitable for SMEs. Qualitative results categorize numerous insights and recommendations into ten distinct categories related to Industry 4.0. Conclusions: This paper provides a structured summary to support newcomers, research institutions, and businesses in effectively initiating and optimizing their Industrsy 4.0 activities.
Design and Implementation of Integral Ergo-Value Stream Mapping in a Metal-Mechanical Company to Improve Ergonomic and Productive Conditions: A Case Study
Authors: Domínguez-Alfaro Denisse , Mendoza-Muñoz Ismael , Montoya-Reyes Mildrend Ivett , Vargas-Bernal Olivia Yessenia , Jacobo-Galicia Gabriela
Published: December 2023

The Integral Ergonomic-Value Stream Mapping (Ergo-VSM) methodology is introduced in this study, which is tailored for the metal-mechanic sector and aims to assess the operational and ergonomic conditions of production processes. The methodology is designed to identify ergonomic risks and propose an improvement plan to increase productivity by integrating ergonomic measurement instruments aligned with official standards and lean manufacturing tools such as VSM and Kaizen. The study, which was conducted in a metal-mechanical MSME (micro, small and medium enterprises), resulted in an 11.8% overall improvement in psychosocial risk factors, a 4.4% increase in productivity with a 1.96-h reduction in cycle time, and a 20% decrease in reported quality rejections. Notably, the study shows that the Integral Ergo-VSM can be implemented in a variety of organizational contexts, ensuring adaptability without jeopardizing the methodology’s core objectives.
Production of Cellulosic Microfibers from Coffee Pulp via Alkaline Treatment, Bleaching and Acid Hydrolysis
Authors: Rodriguez-Quiroz Eliud S. , Olivares-Xometl Octavio , Santacruz-Vázquez Verónica , Santacruz-Vázquez Claudia , Arellanes-Lozada Paulina , Rubio-Rosas Efraín
Published: December 2023

The present work deals with the production of cellulosic microfibers (CMFs) from coffee pulp. The experimental development corresponds to an experimental design of three variables (concentration, temperature and time) of alkaline treatment for delignification, finding that concentration, temperature and time were the most representative variables. Higher delignification was achieved by bleaching cellulosic fibers, followed by acid hydrolysis, thus producing cellulosic fibers with an average diameter of 5.2 µm, which was confirmed using scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDS). An X-ray diffraction (XRD) analysis revealed, via the crystallinity index, the presence of Type I cellulose and removal of lignocellulosic compounds through chemical treatments. The proximate chemical analysis (PChA) of coffee pulp helped to identify 17% of the crude fiber corresponding to the plant cell wall consisting of lignocellulosic compounds. The initial cellulose content of 26.06% increased gradually to 48.74% with the alkaline treatment, to 57.5% with bleaching, and to 64.7% with acid hydrolysis. These results attested to the rich cellulosic content in the coffee pulp.
Rutting Performance Evaluation of BMD Surface Mixtures with Conventional and High RAP Contents under Full-Scale Accelerated Testing
Authors: Tong Bilin , Habbouche Jhony , Flintsch Gerardo W. , Diefenderfer Brian K.
Published: December 2023

The balanced mix design (BMD) constitutes a significant step forward in the pursuit of better-performing asphalt mixtures. This approach/framework offers increased innovative opportunities for the proper design and production of engineered asphalt mixtures without the need to strictly adhere to traditional volumetric requirements. The primary objective of this paper is to conduct a comprehensive investigation of the permanent deformation (rutting) behavior of surface mixtures (SMs) with conventional and high reclaimed asphalt pavement (HRAP) contents through full-scale accelerated testing under incremental loading conditions while accounting for the environmental aging effect. HRAP SMs were designed in this study, marking the initial application of Virginia Department of Transportation (VDOT) BMD special provisions, with attempts to incorporate 45% and even 60% RAP. Results showed that all BMD HRAP mixtures exhibited higher rut depths compared to the control mixture, which can be attributed to the inclusion of high binder contents aimed at enhancing cracking resistance. The asphalt pavement analyzer (APA) rut test and the stress sweep rutting tests were performed on mixtures sampled during production. Correlation analysis revealed significant and strong positive correlations between accelerated pavement testing (APT) and the multilevel laboratory rutting performance tests considered in this study. Finally, while acknowledging the limitations and all the assumptions considered in this study, the correlation analysis recommended refining the VDOT BMD APA rut depth threshold by lowering the current limit of 8 mm to 7 mm to ensure good performing mixtures from a rutting point of view.
Worsening Symptoms Is Associated with Larger Cerebral Blood Flow Abnormalities during Tilt-Testing in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)
Authors: van Campen C. (Linda) M. C. , Rowe Peter C. , Visser Frans C.
Published: December 2023

Background and Objectives: During tilt testing, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) patients experience an abnormal reduction in cerebral blood flow (CBF). The relationship between this CBF reduction and symptom severity has not been examined in detail. Our hypothesis was that ME/CFS severity is related to the degree of the CBF reduction during tilt testing. Materials and Methods: First, from our database, we selected ME/CFS patients who had undergone assessments of ME/CFS symptomatology and tilt tests on the same day, one at the first visit and the second during a follow-up. The change in symptomatology was related to the change in CBF during the tilt test. Second, we combined the data of two previously published studies (n = 219), where disease severity as defined by the 2011 international consensus criteria (ICC) was available but not published. Results: 71 patients were retested because of worsening symptoms. The ICC disease severity distribution (mild-moderate-severe) changed from 51/45/4% at visit-1 to 1/72/27% at follow-up (p < 0.0001). The %CBF reduction changed from initially 19% to 31% at follow-up (p < 0.0001). Of 39 patients with stable disease, the severity distribution was similar at visit-1 (36/51/13%) and at follow-up (33/49/18%), p = ns. The %CBF reduction remained unchanged: both 24%, p = ns. The combined data of the two previously published studies showed that patients with mild, moderate, and severe disease had %CBF reductions of 25, 29, and 33%, respectively (p < 0.0001). Conclusions: Disease severity and %CBF reduction during tilt testing are highly associated in ME/CFS: a more severe disease is related to a larger %CBF reduction. The data suggest a causal relationship where a larger CBF reduction leads to worsening symptoms.