bluuebunny/crossref_metadata_2025 huggingface dataset:
Alto consumo de sódio: impacto na saúde da população brasileira adulta
Authors: Aguiar Millena dos Santos , Bernardo Eliane Duarte de Sousa , Costa Flávia Nunes
Published: November 2021

A aceleração da vida moderna aumenta a busca por facilidades, afetando diretamente a alimentação das pessoas. Nesse contexto, os alimentos processados e industrializados ganham destaque, tendo em vista a praticidade em seus preparos. Um dos problemas em torno desses alimentos está na grande quantidade de sódio em sua composição, tanto para dar mais sabor aos alimentos quanto para conservá-los em suas embalagens. Sendo notório o consumo exagerado desses alimentos, principalmente por parte de adultos. Assim, este trabalho tem como objetivo descrever o impacto do consumo de sódio na população brasileira adulta, relatando as principais consequências à saúde decorrente da sua alta ingestão. Para isso, uma revisão integrativa de literatura foi realizada em um recorte temporal de 2003 a 2021, obtendo resultados que geraram discussões referentes ao sódio no organismo e alimentos, importância da rotulagem e as consequências do alto consumo de sódio. Verificou-se que o alto teor de sódio pode desencadear doenças ligadas à hipertensão, doenças renais, câncer de estômago, dentre outras. E, que apesar de haver normativas específicas acerca da rotulagem de embalagens, ainda é preciso melhorias principalmente em torno da legibilidade dos rótulos, facilitando assim o acesso à leitura destes. Deste modo, observou-se a importância da conscientização da população em torno do tema, pois somente o acesso ao conhecimento e à informação referente ao que se é consumido, se torna possível a diminuição na quantidade da ingestão de sódio, impactando diretamente na qualidade de vida dos adultos brasileiros.
Prevenção e tratamento da constipação intestinal da gestante: sob olhar de fisioterapeutas
Authors: Cardoso Ana Júlia Oliveira , Moura Juliana Braga Facchinetti
Published: November 2021

Objetivo: Identificar as condutas adotadas por fisioterapeutas para prevenção e tratamento da constipação intestinal em gestantes. Metodologia: Trata-se de uma pesquisa de caráter descritivo e exploratório com abordagem qualitativa, que foi realizada de forma não presencial, em formato on-line. A população do estudo foi representada por fisioterapeutas atuantes na área de fisioterapia pélvica na cidade de Vitória da Conquista - BA. Foi utilizado como instrumento um questionário on-line elaborado pelas pesquisadoras na plataforma Google Forms, contendo perguntas para caracterizar o perfil sociodemográfico e ocupacional das participantes. Seguido de perguntas direcionadas ao tema em estudo onde os participantes puderam compartilhar por escrito, suas experiências e condutas adotadas na prevenção e tratamento de mulheres que possuem CI no período gestacional. Resultados: Responderam ao questionário 5 fisioterapeutas do sexo feminino, com idades entre 24 a 44 anos, onde a maioria possuía título de especialista na área de Fisioterapia Pélvica e relataram ter conhecimento e domínio de atuação da situação em estudo. As participantes compartilharam técnicas em comuns de prevenção e tratamento da CI em mulheres no período gravídico. Conclusão: Os resultados desta pesquisa demonstraram que as condutas utilizadas pelas fisioterapeutas aliadas ao envolvimento da equipe multidisciplinar são eficazes para prevenir e tratar a constipação intestinal em gestantes.
Validation of a Thai version of the Hope Scale among patients with acute myocardial infarction
Authors: Inyoo Ampika , Polsook Rapin
Published: November 2021

Background: Hope is essential for patients with acute myocardial infarction, which is often regarded as an active coping for those with life-threatening diseases. Higher hope consistently is related to better health outcomes. However, the instrument for assessing hope has not been previously tested among Thai patients.
Objective: This study aimed to assess the validity and reliability of the Hope Scale among Thai patients with acute myocardial infarction patients.
Methods: This was an instrument validation study conducted from March to December 2020. A total of 213 patients with acute myocardial infarction were included and selected using a simple random sampling. The back-translation method was used to translate an English version to a Thai version of the scale. Principal Component Analysis (PCA) and Cronbach’s alpha were used for construct validity and reliability.
Results: The findings showed that the Cronbach’s alpha of the Thai version of the Hope Scale was acceptable (.75), and the scale consists of four factors, which explained 63.36 % of the total variance. Communalities in each factor ranged from .45 to .84. Overall, the appearance of the factor structures was reasonable and understandable.
Conclusion: The reliability and validity of the Thai version of the Hope Scale were suitable for the measurement of hope in Thai patients. This scale can be used by nurses and others to assess hope in Thai patients with acute myocardial infarction.
Development of Indole Alkaloid-Type Dual Immune Checkpoint Inhibitors Against CTLA-4 and PD-L1 Based on Diversity-Enhanced Extracts
Authors: Suzuki Yoshihide , Ichinohe Keisuke , Sugawara Akihiro , Kida Shinya , Murase Shinya , Zhang Jing , Yamada Osamu , Hattori Toshio , Oshima Yoshiteru , Kikuchi Haruhisa
Published: November 2021

Cancer immunotherapy involves the use of the immune system for cancer treatment. Recently, immune checkpoint-blocking antibodies have become integral for the treatment of some cancers. However, small molecules exhibit advantages over monoclonal antibody drugs, such as cell penetration, long half-life, and low manufacturing costs, and the possibility of oral administration. Thus, it is imperative to develop small-molecule immune checkpoint inhibitors. Previously, we have screened a library of synthetic indole-alkaloid-type compounds, which are produced by diversity-enhanced extracts of Japanese cornelian cherry, and reported that an unnatural pentacyclic compound inhibits CTLA-4 gene expression. In this study, immune checkpoint inhibitors with increased potency were developed by introducing substituents and conversion of functional groups based on the unnatural pentacyclic compound. The developed compounds suppressed not only CTLA-4 and PD-L1 gene expression but also protein expression on the cell surface. Their efficacy was not as potent as that of the existing small-molecule immune checkpoint inhibitors, but, to the best of our knowledge, the developed compounds are the first reported dual small-molecule inhibitors of CTLA-4 and PD-L1.
Aggregated Technical Commercial and Collection Loss Mitigation Through a Smart Metering Application Strategy
Authors: Odje Mathias , Uhunmwangho Roland , Okedu Kenneth E.
Published: November 2021

The privatization exercise that led to the creation of 11 distribution companies (Discos) is yet to yield the result of meeting the electricity supply needs of Nigeria. It is in realization of this fact that the Federal Government of Nigeria recently made different proposals and regulations to increase efficiency, availability, and competitiveness within the power industry. This article establishes a technical gain ratio to measure the impact of scaling up smart metering on the aggregated technical commercial and collection (ATC&C) losses. The ATC&C loss is the difference between the amount of electricity received by a distribution company from the transmission company and the amount of electricity for which it invoices its customers plus the adjusted collections loss. To achieve this objective, reviews of historical data of the Discos ATC&C losses and customers’ metering records as published by the Nigerian Electricity Regulatory Commission (NERC) for 2015, 2016, 2017, 2018, and 2020 were carried out. In addition, efforts were made to mathematically model the relationship between % metering and % ATC&C losses as this was the framework to help carry out effective forecasts and analyses of the study in order to show the impact level of the strategy employed. One of the salient technical contributions of this article was that it established that for every 1% increase in metering installation, there is a proportionate 0.8% decrease in ATC&C losses, provided all other factors responsible for technical and commercial losses remain constant. Consequently, improved ATC&C loss reduction would be achieved if Discos adopt a combination of other strategies that would ensure reduction in technical and commercial losses in addition to aggressive deployment of meter assets. However, in practice, factors causing technical and commercial losses are never constant as system components depreciate/burn out with time, energy theft, and pilferage, and meter tamper/bypass is on the increase daily; meter deployment is not aggressive enough to match utility customers’ growth. Hence, the adoption of combined modern strategies in addition to aggressive metering in tandem with customers’ growth has to be employed by Discos in a deliberate attempt to reduce ATC&C losses.
A Comparison of Size, Shape, and Fractal Diversity Between Coral Rubble Sampled From Natural and Artificial Coastlines Around Okinawa Island, Japan
Authors: Masucci Giovanni D. , Biondi Piera , Reimer James D.
Published: November 2021

Substrate surface area and fractal complexity have been reported to influence the abundance and diversity of mobile cryptic animal communities. Surfaces with higher fractal dimensions not only offer additional space for colonization, but bias space availability toward smaller size ranges, increasing the number of available niches. Conversely, smaller surface areas tend to be associated with a decrease in abundances while reduced fractal complexities may support less diversity in the benthic community. In this study, we compared morphological parameters between rubble sampled from rubble mounds accumulated at the seaward side of artificial breakwaters and rubble sampled from a nearby location with no breakwaters (=control site). The purpose of this work was to establish a methodology for comparing the surface area and fractal complexity of coral rubble fragments using easily available equipment that could be efficiently utilized during field work. Rubble fragments were individually weighed and photographed in controlled light conditions. Each photograph was then analyzed using ImageJ software. Rubble pieces from each photograph went through segmentation, the separation of sample outlines from the picture background, before being measured and analyzed for surface area, width, and length (size estimators), and circularity, aspect ratio, roundness, solidity, and Feret properties (shape estimators). Surface fractal complexity was also measured, using the box counting method on segmented rubble surfaces. We observed lowered surface areas, weight, and fractal complexity for rubble fragments sampled at the breakwater. We demonstrate how this method could be used to compare coral rubble from a variety of environmental conditions, thus becoming a useful aid in environmental monitoring, in addition to adding important information to the collection and analyses of biological data.
VTG-Net: A CNN Based Vessel Topology Graph Network for Retinal Artery/Vein Classification
Authors: Mishra Suraj , Wang Ya Xing , Wei Chuan Chuan , Chen Danny Z. , Hu X. Sharon
Published: November 2021

From diagnosing cardiovascular diseases to analyzing the progression of diabetic retinopathy, accurate retinal artery/vein (A/V) classification is critical. Promising approaches for A/V classification, ranging from conventional graph based methods to recent convolutional neural network (CNN) based models, have been known. However, the inability of traditional graph based methods to utilize deep hierarchical features extracted by CNNs and the limitations of current CNN based methods to incorporate vessel topology information hinder their effectiveness. In this paper, we propose a new CNN based framework, VTG-Net (vessel topology graph network), for retinal A/V classification by incorporating vessel topology information. VTG-Net exploits retinal vessel topology along with CNN features to improve A/V classification accuracy. Specifically, we transform vessel features extracted by CNN in the image domain into a graph representation preserving the vessel topology. Then by exploiting a graph convolutional network (GCN), we enable our model to learn both CNN features and vessel topological features simultaneously. The final predication is attained by fusing the CNN and GCN outputs. Using a publicly available AV-DRIVE dataset and an in-house dataset, we verify the high performance of our VTG-Net for retinal A/V classification over state-of-the-art methods (with ~2% improvement in accuracy on the AV-DRIVE dataset).
Role of Imaging in Diagnosis and Management of COVID-19: A Multiorgan Multimodality Imaging Review
Authors: Varadarajan Vinithra , Shabani Mahsima , Ambale Venkatesh Bharath , Lima Joao A. C.
Published: November 2021

In this pandemic of Coronavirus disease 2019 (COVID-19), a vast proportion of healthcare resources, including imaging tools, have been dedicated to the management of affected patients; yet, the frequent reports of unknown presentations and complications of disease over time have been changing the usual standard of care and resource allocation in health centers. As of now, we have witnessed multisystemic symptoms requiring the collaboration of different clinical teams in COVID-19 patients' care. Compared to previous viral pandemics, imaging modalities are now playing an essential role in the diagnosis and management of patients. This widespread utility of imaging modalities calls for a deeper understanding of potential radiologic findings in this disease and identifying the most compatible imaging protocol with safety precautions. Although initially used for respiratory tract evaluation, imaging modalities have also been used for cardiovascular, neurologic, and gastrointestinal evaluation of patients with COVID-19. In this narrative review article, we provide multimodality and multisystemic review of imaging techniques and features that can aid in the diagnosis and management of COVID-19 patients.
The Dynamic of Extracellular Vesicles in Patients With Subacute Stroke: Results of the “Biomarkers and Perfusion—Training-Induced Changes After Stroke” (BAPTISe) Study
Authors: Jödicke Ruben A. , Huo Shufan , Kränkel Nicolle , Piper Sophie K. , Ebinger Martin , Landmesser Ulf , Flöel Agnes , Endres Matthias , Nave Alexander H.
Published: November 2021

Objective: Extracellular vesicles (EV) are sub-1 μm bilayer lipid coated particles and have been shown play a role in long-term cardiovascular outcome after ischemic stroke. However, the dynamic change of EV after stroke and their implications for functional outcome have not yet been elucidated.

Methods: Serial blood samples from 110 subacute ischemic stroke patients enrolled in the prospective BAPTISe study were analyzed. All patients participated in the PHYS-STROKE trial and received 4-week aerobic training or relaxation sessions. Levels of endothelial-derived (EnV: Annexin V+, CD45–, CD41–, CD31+/CD144+/CD146+), leukocyte-derived (LV: Annexin V+, CD45+, CD41–), monocytic-derived (MoV: Annexin V+, CD41–, CD14+), neuronal-derived (NV: Annexin V+, CD41–, CD45–, CD31–, CD144–, CD146–, CD56+/CD171+/CD271+), and platelet-derived (PV: Annexin V+, CD41+) EV were assessed via fluorescence-activated cell sorting before and after the trial intervention. The levels of EV at baseline were dichotomized at the 75th percentile, with the EV levels at baseline above the 75th percentile classified as “high” otherwise as “low.” The dynamic of EV was classified based on the difference between baseline and post intervention, defining increases above the 75th percentile as “high increase” otherwise as “low increase.” Associations of baseline levels and change in EV concentrations with Barthel Index (BI) and cardiovascular events in the first 6 months post-stroke were analyzed using mixed model regression analyses and cox regression.

Results: Both before and after intervention PV formed the largest population of vesicles followed by NV and EnV. In mixed-model regression analyses, low NV [−8.57 (95% CI −15.53 to −1.57)] and low PV [−6.97 (95% CI −13.92 to −0.01)] at baseline were associated with lower BI in the first 6 months post-stroke. Patients with low increase in NV [8.69 (95% CI 2.08–15.34)] and LV [6.82 (95% CI 0.25–13.4)] were associated with reduced BI in the first 6 months post-stroke. Neither baseline vesicles nor their dynamic were associated with recurrent cardiovascular events.

Conclusion: This is the first report analyzing the concentration and the dynamic of EV regarding associations with functional outcome in patients with subacute stroke. Lower levels of PV and NV at baseline were associated with a worse functional outcome in the first 6 months post-stroke. Furthermore, an increase in NV and LV over time was associated with worse BI in the first 6 months post-stroke. Further investigation of the relationship between EV and their dynamic with functional outcome post-stroke are warranted.

Clinical Trial Registration:clinicaltrials.gov/, identifier: NCT01954797.
Suppressor of Cytokine Signaling 2 Regulates Retinal Pigment Epithelium Metabolism by Enhancing Autophagy
Authors: Liu Xi-Yuan , Lu Rui , Chen Jing , Wang Jie , Qian Hong-Mei , Chen Gang , Wu Rong-Han , Chi Zai-Long
Published: November 2021

Retinal pigment epithelium (RPE) serves critical functions in maintaining retinal homeostasis. An important function of RPE is to degrade the photoreceptor outer segment fragments daily to maintain photoreceptor function and longevity throughout life. An impairment of RPE functions such as metabolic regulation leads to the development of age-related macular degeneration (AMD) and inherited retinal degenerative diseases. As substrate recognition subunit of a ubiquitin ligase complex, suppressor of cytokine signaling 2 (SOCS2) specifically binds to the substrates for ubiquitination and negatively regulates growth hormone signaling. Herein, we explore the role of SOCS2 in the metabolic regulation of autophagy in the RPE cells. SOCS2 knockout mice exhibited the irregular morphological deposits between the RPE and Bruch’s membrane. Both in vivo and in vitro experiments showed that RPE cells lacking SOCS2 displayed impaired autophagy, which could be recovered by re-expressing SOCS2. SOCS2 recognizes the ubiquitylated proteins and participates in the formation of autolysosome by binding with autophagy receptors and lysosome-associated membrane protein2 (LAMP-2), thereby regulating the phosphorylation of glycogen synthase kinase 3β (GSK3β) and mammalian target of rapamycin (mTOR) during the autophagy process. Our results imply that SOCS2 participates in ubiquitin-autophagy-lysosomal pathway and enhances autophagy by regulating GSK3β and mTOR. This study provides a potential therapeutic target for AMD.
Prognostic Relevance of BRCA1 Expression in Survival of Patients With Cervical Cancer
Authors: Paik E Sun , Chang Chi-Son , Chae Ye Lin , Oh So Young , Byeon Sun-Ju , Kim Chul Jung , Lee Yoo-Young , Kim Tae-Joong , Lee Jeong-Won , Kim Byoung-Gie , Choi Chel Hun
Published: November 2021

Objective

BRCA1 expression can be lost by a variety of mechanisms including germline or somatic mutation and promotor hypermethylation. Given the potential importance of BRCA1 loss as a predictive and prognostic biomarker in several cancers, the objective of this study was to investigate BRCA1 expression using immunohistochemistry (IHC) in cervical cancer and its possible prognostic relevance.

Methods

Seventy patients with cervical cancer were enrolled in this study. Samples from each tumor were stained for BRCA1 and reviewed independently by gynecologic pathologists blinded to the BRCA status. Kaplan–Meier methods were used to estimate overall survival according to BRCA1 expression. Differentially expressed genes (DEGs) by BRCA1 expression were selected using GSE44001 dataset, which included 300 samples treated with radical hysterectomy. In addition, cox regression analysis with backward elimination was performed to select independent prognostic markers. Gene set enrichment analysis (GSEA) was done using these DEGs.

Results

BRCA1 IHC was positive in 62.9% (44/70) of cases. Patients with BRCA1 expression showed better overall survival (100% vs. 76.2%, HR 0.20, 95% CI 0.04 – 0.99, p = 0.028) than those without BRCA1 expression. Analysis of gene expression profiles according to BRCA1 expression identified 321 differentially expressed mRNAs. Gene set enrichment analysis results showed two dysregulated pathways (VEGF_A_UP.V1_DN and E2F1_UP.V1_UP). Of these DEGs, alterations of 20 gene signatures were found to be independently associated with survival outcomes of patients.

Conclusions

BRCA1 expression in cervical cancer tissue is associated with survival. In addition, the identification of specific gene alterations associated with BRCA1 expression could help to provide individualized prediction in these patients.
LY294002 Is a Promising Inhibitor to Overcome Sorafenib Resistance in FLT3-ITD Mutant AML Cells by Interfering With PI3K/Akt Signaling Pathway
Authors: Huang Amin , Zeng Peiting , Li Yinguang , Lu Wenhua , Lai Yaoming
Published: November 2021

Internal tandem duplications (ITD) mutation within FMS-like tyrosine kinase 3 (FLT3), the most frequent mutation happens in almost 20% acute myeloid leukemia (AML) patients, always predicts a poor prognosis. As a small molecule tyrosine kinase inhibitor, sorafenib is clinically used for the treatment of advanced renal cell carcinoma (RCC), hepatocellular carcinoma (HCC), and differentiated thyroid cancer (DTC), with its preclinical and clinical activity demonstrated in the treatment of Fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) mutant AML. Even though it shows a rosy future in the AML treatment, the short response duration remains a vital problem that leads to treatment failure. Rapid onset of drug resistance is still a thorny problem that we cannot overlook. Although the mechanisms of drug resistance have been studied extensively in the past years, there is still no consensus on the exact reason for resistance and without effective therapeutic regimens established clinically. My previous work reported that sorafenib-resistant FLT3-ITD mutant AML cells displayed mitochondria dysfunction, which rendered cells depending on glycolysis for energy supply. In my present one, we further illustrated that losing the target protein FLT3 and the continuously activated PI3K/Akt signaling pathway may be the reason for drug resistance, with sustained activation of PI3K/AKT signaling responsible for the highly glycolytic activity and adenosine triphosphate (ATP) generation. PI3K inhibitor, LY294002, can block PI3K/AKT signaling, further inhibit glycolysis to disturb ATP production, and finally induce cell apoptosis. This finding would pave the way to remedy the FLT3-ITD mutant AML patients who failed with FLT3 targeted therapy.
Tocilizumab in Systemic Juvenile Idiopathic Arthritis: Response Differs by Disease Duration at Medication Initiation and by Phenotype of Disease
Authors: Yan Xin , Tang Wenjing , Zhang Zhiyong , Zhang Yu , Luo Chong , Tang Xuemei
Published: November 2021

Objective: We performed a single-center retrospective study to determine the different efficacy of tocilizumab (TCZ) in the early and late stages and in three phenotypic subgroups (monocyclic, polycyclic, and persistent) of systemic juvenile idiopathic arthritis (sJIA).

Methods: Clinical and serological parameters of 77 sJIA patients treated by TCZ were collected from November 1, 2013 to May 1, 2019. Patients were grouped based on the duration group A < 6 months (n = 41) and group B > 6 months (n = 36) and divided into three phenotypes: monocyclic (n = 12), polycyclic (n = 14), and persistent (n = 51) course.

Results: At baseline, group A had pronounced ESR, fever less active arthritis than group B (p < 0.05). After 12 weeks of therapy, TCZ alleviated fever, ESR, CRP, and systemic-onset juvenile arthritis disease activity score-27 (sJADAS27) in both group A and group B (p>0.05), while the efficacy of TCZ in relieving active arthritis in group A was better than that in group B (p<0.05). After 1 year of TCZ therapy, it showed that patients with monocyclic phenotype had the highest clinical response rate (91.7%, odds ratio = 0, 95% CI: 24–24, p = 0.00), followed by the polycyclic (28.6%, odds ratio = 2.1, 95% CI: 10.5–18.8, p = 0.00) and the persistent course (9.8%, odds ratio = 1.2, 95% CI: 9.5–13.8, p = 0.00).

Conclusion: TCZ can quickly relieve fever and inflammation, especially when patients have less active arthritis with shorter disease duration. The long-term efficacy of TCZ is related to the phenotypes, among which the monocyclic is the best, and the persistent is the worst.
Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
Authors: Zhan Yi , Deng Bing , Wu Huixian , Xu Changpeng , Wang Ruiying , Li Wenqiang , Pan Zhixiong
Published: November 2021

Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and biomineralization technique that precisely controls the fibrous structure in liquid LC phase. The enriched-LC composites have superior mineralization ability than neat PLLA; furthermore BMSC cells were inoculated onto the HAP-PLLA/LC with hydroxyapatite (HAP) composite scaffold to test the capability for osteogenesis in vitro. The results show that the PLLA/LC with HAP produced by mineralization leads to better cell compatibility, which is beneficial to cell proliferation, osteogenic differentiation, and expression of the angiogenic CD31 gene. Moreover, in vivo studies showed that the HAP-PLLA/LC scaffold with a bone-like environment significantly accelerates new and mature lamellar bone formation by development of a microenvironment for vascularized bone regeneration. Thus, this bionic composite scaffold in an LC state combining osteogenesis with vascularized activities is a promising biomaterial for bone regeneration in defective areas.
An Evolutionary Study of Carex Subg. Psyllophorae (Cyperaceae) Sheds Light on a Strikingly Disjunct Distribution in the Southern Hemisphere, With Emphasis on Its Patagonian Diversification
Authors: Benítez-Benítez Carmen , Otero Ana , Ford Kerry A. , García-Moro Pablo , Donadío Sabina , Luceño Modesto , Martín-Bravo Santiago , Jiménez-Mejías Pedro
Published: November 2021

Carex subgenus Psyllophorae is an engaging study group due to its early diversification compared to most Carex lineages, and its remarkable disjunct distribution in four continents corresponding to three independent sections: sect. Psyllophorae in Western Palearctic, sect. Schoenoxiphium in Afrotropical region, and sect. Junciformes in South America (SA) and SW Pacific. The latter section is mainly distributed in Patagonia and the Andes, where it is one of the few Carex groups with a significant in situ diversification. We assess the role of historical geo-climatic events in the evolutionary history of the group, particularly intercontinental colonization events and diversification processes, with an emphasis on SA. We performed an integrative study using phylogenetic (four DNA regions), divergence times, diversification rates, biogeographic reconstruction, and bioclimatic niche evolution analyses. The crown age of subg. Psyllophorae (early Miocene) supports this lineage as one of the oldest within Carex. The diversification rate probably decreased over time in the whole subgenus. Geography seems to have played a primary role in the diversification of subg. Psyllophorae. Inferred divergence times imply a diversification scenario away from primary Gondwanan vicariance hypotheses and suggest long-distance dispersal-mediated allopatric diversification. Section Junciformes remained in Northern Patagonia since its divergence until Plio-Pleistocene glaciations. Andean orogeny appears to have acted as a northward corridor, which contrasts with the general pattern of North-to-South migration for temperate-adapted organisms. A striking niche conservatism characterizes the evolution of this section. Colonization of the SW Pacific took place on a single long-distance dispersal event from SA. The little ecological changes involved in the trans-Pacific disjunction imply the preadaptation of the group prior to the colonization of the SW Pacific. The high species number of the section results from simple accumulation of morphological changes (disparification), rather than shifts in ecological niche related to increased diversification rates (radiation).
Photopic and Mesopic Contrast Sensitivity Function in the Presence of Glare and the Effect of Filters in Young Healthy Adults
Authors: Domínguez-Vicent Alberto , Helghe Emma , Wahlberg Ramsay Marika , Venkataraman Abinaya Priya
Published: November 2021

Purpose: The aim of this study was to evaluate the effect of four different filters on contrast sensitivity under photopic and mesopic conditions with and without glare.

Methods: A forced choice algorithm in a Bayesian psychophysical procedure was utilized to evaluate the spatial luminance contrast sensitivity. Five different spatial frequencies were evaluated: 1.5, 3, 6, 12, and 18 cycles per degree (cpd). The measurements were performed under 4 settings: photopic and mesopic luminance with glare and no glare. Two long pass filters (LED light reduction and 511nm filter) and two selective absorption filters (ML41 and emerald filter) and a no filter condition were evaluated. The measurements were performed in 9 young subjects with healthy eyes.

Results: For the no filter condition, there was no difference between glare and no glare settings for the photopic contrast sensitivity measurements whereas in the mesopic setting, glare reduced the contrast sensitivity significantly at all spatial frequencies. There was no statistically significant difference between contrast sensitivity measurements obtained with different filters under both photopic conditions and the mesopic glare condition. In the mesopic no glare condition, the contrast sensitivity at 6 cpd with 511, ML41 and emerald filters was significantly reduced compared to no filter condition (p = 0.045, 0.045, and 0.071, respectively). Similarly, with these filters the area under the contrast sensitivity function in the mesopic no glare condition was also reduced. A significant positive correlation was seen between the filter light transmission and the average AULCSF in the mesopic non-glare condition.

Conclusion: The contrast sensitivity measured with the filters was not significantly different than the no filter condition in photopic glare and no glare setting as well as in mesopic glare setting. In mesopic setting with no glare, filters reduced contrast sensitivity.
Enhancing Upper Limb Rehabilitation of Stroke Patients With Virtual Reality: A Mini Review
Authors: Bui Julie , Luauté Jacques , Farnè Alessandro
Published: November 2021

Upper limb motor impairment following stroke is a common condition that impacts significantly the independence and quality of life of stroke survivors. In recent years, scholars have massively turned to virtual reality (VR) to develop more effective rehabilitation approaches. VR systems are promising tools that can help patients engage in intensive, repetitive and task-oriented practice using new technologies to promote neuroplasticity and recovery. Multiple studies have found significant improvements in upper limb function for patients using VR in therapy, but the heterogeneity of methods and tools employed make the assessment of VR efficacy difficult. Here we aimed to assess the potential of VR as a therapy tool for upper limb motor impairment and to provide initial assessment of what is the added value of using VR to both patients and clinicians. Our mini-review focuses the work published since the Cochrane review (2017) and suggests that VR may be particularly effective when used in combination to conventional rehabilitation approaches. We also highlight key features integrated in VR systems that appear to influence rehabilitation and can help maximizing therapy outcomes, if exploited properly. We conclude that although promising results have already been gathered, more focused research is needed to determine the optimal conditions to implement VR in clinical settings in order to enhance therapy and to better define and leverage the true potential of VR. The rapid pace of technological development and increasing research interest toward VR-based therapy will help providing extensive knowledge and lead to rapid advancements in the near future.
Womens’ Career Progression in an Australian Regional University
Authors: White Kate , Goriss-Hunter Anitra
Published: November 2021

This article examines the link between terms of employment (full time, part time and casual) at an Australian regional university and women’s career progression. The literature identifies lack of transparency in recruitment, promotion and retention; mobility and location; and management perceptions of women’s choice to work flexibly as factors impacting on career progression. However, the voices of women working in regional universities and particularly those of professional staff are often not present in current research. This study moves towards addressing this research deficit. Feminist institutionalism is used to analyse the relationship between national legislation, university policies and informal institutional practices in relation to women’s career progression In early 2020, twenty-one women provided written responses to questions on the link between terms of employment and career progression. The main findings tend to support other research about women working in universities; that is, carers need flexible work arrangements. But there are particular differences for women in regional universities who have to travel between dispersed campuses, which brings an added dimension of complexity to career progression. Their choices about terms of employment and fulfilling carer responsibilities resulted in insecure employment for some participants which had an impact on wellbeing and confidence. In addition, care/household responsibilities and the choice to work flexibly had a negative effect on career progression, and managers did not necessarily support flexible work options (despite national legislation that enables employees with child care responsibilities to negotiate flexible work arrangements with managers, and institutional gender equality policies).
The Acceptability and Appropriateness of a Collaborative, Sport-Centered Mental Health Service Delivery Model for Competitive, and High-Performance Athletes
Authors: Van Slingerland Krista J. , Durand-Bush Natalie
Published: November 2021

The purpose of this study was to evaluate the acceptability and appropriateness of a sport-centered, collaborative mental health service delivery model implemented within the Canadian Center for Mental Health and Sport (CCMHS) over a period of 16 months. The study is situated within a larger Participatory Action Research (PAR) project to design, implement and evaluate the CCMHS. Primary data were collected from CCMHS practitioners (n = 10) and service-users (n = 6) through semi-structured interviews, as well as from CCMHS stakeholders (n = 13) during a project meeting, captured via meeting minutes. Secondary data derived from documents (e.g., clinical, policy, procedural; n = 48) created by the CCMHS team (i.e., practitioners, stakeholders, board of directors) during the Implementation Phase of the project were reviewed and analyzed to triangulate the primary data. The Framework Method was used to organize, integrate and interpret the dataset. Overall, results indicate that both practitioners and service-users found the model to be both acceptable and appropriate. In particular, practitioners' knowledge and experience working in sport, a robust intake process carried out by a centralized Care Coordinator, and the ease and flexibility afforded by virtual care delivery significantly contributed to positive perceptions of the model. Some challenges associated with interprofessional collaboration and mental health care costs were highlighted and perceived as potentially hindering the model's acceptability and appropriateness.
Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to Botrytis fabae Infection
Authors: Castillejo María Ángeles , Villegas-Fernández Ángel M. , Hernández-Lao Tamara , Rubiales Diego
Published: November 2021

Chocolate spot, which is caused by the necrotrophic fungus Botrytis fabae, is a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (Vicia faba). Although chemical control of this disease is an option, it has serious economic and environmental drawbacks that make resistant cultivars a more sensible choice. The molecular mechanisms behind the defense against B. fabae are poorly understood. In this work, we studied the leave proteome in two faba bean genotypes that respond differently to B. fabae in order to expand the available knowledge on such mechanisms. For this purpose, we used two-dimensional gel electrophoresis (2DE) in combination with Matrix-Assisted Laser Desorption/Ionization (MALDI-TOF/TOF). Univariate statistical analysis of the gels revealed 194 differential protein spots, 102 of which were identified by mass spectrometry. Most of the spots belonged to proteins in the energy and primary metabolism, degradation, redox or response to stress functional groups. The MS results were validated with assays of protease activity in gels. Overall, they suggest that the two genotypes may respond to B. fabae with a different PSII protein repair cycle mechanism in the chloroplast. The differences in resistance to B. fabae may be the result of a metabolic imbalance in the susceptible genotype and of a more efficient chloroplast detoxification system in the resistant genotype at the early stages of infection.
Antifungal and Anti-Biofilm Effects of Caffeic Acid Phenethyl Ester on Different Candida Species
Authors: Alfarrayeh Ibrahim , Pollák Edit , Czéh Árpád , Vida András , Das Sourav , Papp Gábor
Published: November 2021

This study investigated the effect of CAPE on planktonic growth, biofilm-forming abilities, mature biofilms, and cell death of C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis strains. Our results showed a strain- and dose-dependent effect of CAPE on Candida, and the MIC values were between 12.5 and 100 µg/mL. Similarly, the MBIC values of CAPE ranging between 50 and 100 µg/mL highlighted the inhibition of the biofilm-forming abilities in a dose-dependent manner, as well. However, CAPE showed a weak to moderate biofilm eradication ability (19-49%) on different Candida strains mature biofilms. Both caspase-dependent and caspase-independent apoptosis after CAPE treatment were observed in certain tested Candida strains. Our study has displayed typical apoptotic hallmarks of CAPE-induced chromatin margination, nuclear blebs, nuclear condensation, plasma membrane detachment, enlarged lysosomes, cytoplasm fragmentation, cell wall distortion, whole-cell shrinkage, and necrosis. In conclusion, CAPE has a concentration and strain-dependent inhibitory activity on viability, biofilm formation ability, and cell death response in the different Candida species.
Correlation between Previous Antibiotic Exposure and COVID-19 Severity. A Population-Based Cohort Study
Authors: Llor Carl , Ouchi Dan , Giner-Soriano Maria , García-Sangenís Ana , Bjerrum Lars , Morros Rosa
Published: November 2021

We examined the correlation between previous antibiotic exposure and COVID-19 severity using a population-based observational matched cohort study with patient level data obtained for more than 5.8 million people registered in SIDIAP in Catalonia, Spain. We included all patients newly diagnosed with COVID-19 from March to June 2020 and identified all their antibiotic prescriptions in the previous two years. We used a composite severity endpoint, including pneumonia, hospital admission and death due to COVID-19. We examined the influence of high antibiotic exposure (>4 regimens), exposure to highest priority critically important antimicrobials (HPCIA) and recent exposure. Potential confounders were adjusted by logistic regression. A total of 280,679 patients were diagnosed with COVID-19, 146,656 of whom were exposed to at least one antibiotic course (52.3%) during the preceding two years. A total of 25,222 presented severe COVID-19 infection (9%), and the risk of severity was highest among those exposed to antibiotics (OR 1.12; 95% CI: 1.04–1.21). Among all individuals exposed to antibiotics, high, recent and exposure to HPCIAs were correlated with increased COVID severity (OR 1.19; 95% CI: 1.14–1.26; 1.41; 95% CI: 1.36–1.46; and 1.35; 95% CI: 1.30–1.40, respectively). Our findings confirm a significant correlation between previous antibiotic exposure and increased severity of COVID-19 disease.
Small RNA GcvB Regulates Oxidative Stress Response of Escherichia coli
Authors: Ju Xian , Fang Xingxing , Xiao Yunzhu , Li Bingyu , Shi Ruoping , Wei Chaoliang , You Conghui
Published: November 2021

Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-resolution RNA-seq assay, we compared the transcriptomes of a wild-type Escherichia coli K-12 strain and its gcvB deletion derivative grown in minimal medium and identified putative targets responding to GcvB, including flu, a determinant gene of auto-aggregation. The following molecular studies and the enhanced auto-aggregation ability of the gcvB knockout strain further substantiated the induced expression of these genes. Intriguingly, the reduced expression of OxyR (the oxidative stress regulator) in the gcvB knockout strain was identified to account for the increased expression of flu. Additionally, GcvB was characterized to up-regulate the expression of OxyR at the translational level. Accordingly, compared to the wild type, the GcvB deletion strain was more sensitive to oxidative stress and lost some its ability to eliminate endogenous reactive oxygen species. Taken together, we reveal that GcvB regulates oxidative stress response by up-regulating OxyR expression. Our findings provide an insight into the diversity of GcvB regulation and add an additional layer to the regulation of OxyR.
Long-Term PDE-5A Inhibition Improves Myofilament Function in Left and Right Ventricular Cardiomyocytes through Partially Different Mechanisms in Diabetic Rat Hearts
Authors: Bódi Beáta , Kovács Árpád , Gulyás Hajnalka , Mártha Lilla , Tóth Attila , Mátyás Csaba , Barta Bálint András , Oláh Attila , Merkely Béla , Radovits Tamás , Papp Zoltán
Published: November 2021

Heart failure with preserved ejection fraction (HFpEF) and right ventricular (RV) dysfunction are frequent complications of diabetic cardiomyopathy. Here we aimed to characterize RV and left ventricular (LV) remodeling and its prevention by vardenafil (a long-acting phosphodiesterase-5A (PDE-5A) inhibitor) administration in a diabetic HFpEF model. Zucker Diabetic Fatty (ZDF) and control, ZDF Lean (Lean) male rats received 10 mg/kg vardenafil (ZDF + Vard; Lean + Vard) per os, on a daily basis for a period of 25 weeks. In vitro force measurements, biochemical and histochemical assays were employed to assess cardiomyocyte function and signaling. Vardenafil treatment increased cyclic guanosine monophosphate (cGMP) levels and decreased 3-nitrotyrosine (3-NT) levels in the left and right ventricles of ZDF animals, but not in Lean animals. Cardiomyocyte passive tension (Fpassive) was higher in LV and RV cardiomyocytes of ZDF rats than in those receiving preventive vardenafil treatment. Levels of overall titin phosphorylation did not differ in the four experimental groups. Maximal Ca2+-activated force (Fmax) of LV and RV cardiomyocytes were preserved in ZDF animals. Ca2+-sensitivity of isometric force production (pCa50) was significantly higher in LV (but not in RV) cardiomyocytes of ZDF rats than in their counterparts in the Lean or Lean + Vard groups. In accordance, the phosphorylation levels of cardiac troponin I (cTnI) and myosin binding protein-C (cMyBP-C) were lower in LV (but not in RV) cardiomyocytes of ZDF animals than in their counterparts of the Lean or Lean + Vard groups. Vardenafil treatment normalized pCa50 values in LV cardiomyocytes, and it decreased pCa50 below control levels in RV cardiomyocytes in the ZDF + Vard group. Our data illustrate partially overlapping myofilament protein alterations for LV and RV cardiomyocytes in diabetic rat hearts upon long-term PDE-5A inhibition. While uniform patterns in cGMP, 3-NT and Fpassive levels predict identical effects of vardenafil therapy for the diastolic function in both ventricles, the uneven cTnI, cMyBP-C phosphorylation levels and pCa50 values implicate different responses for the systolic function.
Application of Cornelian Cherry (Cornus mas L.) Peel in Probiotic Ice Cream: Functionality and Viability during Storage
Authors: Haghani Shaghayegh , Hadidi Milad , Pouramin Shiva , Adinepour Fateme , Hasiri Zahra , Moreno Andrés , Munekata Paulo E. S. , Lorenzo José M.
Published: November 2021

In this study, cornelian cherry (Cornus mas L.) peel (CCP) was incorporated into a probiotic ice cream formulation containing Bifidobacterium lactis to investigate the potential effect of CCP on the viability of B. lactis in the ice cream after simulated gastrointestinal stress and during 120 days of storage. Furthermore, the effect of the addition of CCP (3, 6, and 9%) on bioactive compounds, antioxidant activity, and physicochemical and sensory attributes of the ice cream was evaluated. The results showed that the addition of CCP significantly enhanced vitamin C, total polyphenols, total anthocyanin content, and antioxidant activity of the ice cream. During frozen storage of the ice cream, phenolic compounds and anthocyanins were quite stable, but vitamin C significantly decreased. The addition of CCP had no significant effect on the viability of B. lactis throughout the freezing process, but increments of 6% and 9% CCP increased the viability of B. lactis in the ice cream and after simulated gastrointestinal processes in all storage periods. These findings imply that CCP is a promising candidate to be used for producing functional ice cream.
Editorial on Anticancer Antioxidants
Authors: Sunjic Suzana Borovic , Zarkovic Neven
Published: November 2021

The current concepts of biomedicine consider oxidative stress to be one of crucial pathophysiological processes behind major stress- and age-associated diseases, including cancer [...]