bluuebunny/crossref_metadata_2025 huggingface dataset:
An Effective Staff Scheduling for Shift Workers in Social Welfare Facilities for the Disabled
Authors: Ryu Hee Jun , Jo Ye Na , Lee Won Jun , Cheong Ji Won , Moon Boo Yong , Ko Young Dae
Published: January 2023

The efficient management of social worker personnel is important since it involves a huge portion in its operations. However, the burnout and turnover rates of social workers are very high, which is due to dissatisfaction with the irregular and unequal schedules, despite the continuous improvement in the treatment of social workers and the enactment of work-related legislation in Korea. This means that changes in policy do not significantly contribute to improving worker satisfaction, which shows the necessity of the strategies to prevent the turnover of workers. Therefore, this study aims to propose a strategy for the staff scheduling of workers that considers the fairness in the shift distribution among workers and the individual preference for shift work by using the linear programming. A survey about the preferences for shift work is conducted that targeted the employees of a welfare facility in Korea to enhance the practicality of the model. The effectiveness and applicability of the developed mathematical model are verified by deriving a deterministic schedule for a worker via the system parameters that were obtained based on the survey and the rules of the welfare facility in the numerical experiment. Compared to the conventional schedule, the derived schedule shows an improvement in the deviations in the number of shifts workers and a reflection of the personal preferences. This can raise the social worker’s satisfaction, which will decrease intention on burnouts and turnover. It will consequently facilitate on managing human resources in welfare facilities.
Simulation Framework for Real-Time PHM Applications in a System-of-Systems Environment
Authors: Dingeldein Lorenz
Published: January 2023

While the growth of unmanned aerial vehicle (UAV) usage over the next few years is indisputable, cooperative operation strategies for UAV swarms have gained great interest in the research community. Mission capabilities increase while contingencies can be mitigated through intelligent management between the operating swarm and the available fleet. The importance of observing the system reliability and of risk assessment grows because the dysfunction of one asset within a system of systems endangers the superordinate mission goals of the operating UAV swarm. Thus, not only is trajectory planning beneficial for usage optimization, but prognostic and health management (PHM) methods, including diagnostics and prognostics, also enable situational awareness and condition-driven asset management to achieve higher mission reliability. The novelty of this work is the observation of asset states based upon a generically modeled multi-component degradation behavior and the integration of PHM methods with real-time capabilities in order to support decision making during mission execution in a highly dynamic and event-based environment. The developed simulation enables the testing and comparison of different maintenance strategies that are integrated into the simulation to show and discuss the effectiveness and benefits of real-time-capable PHM methods.
Oil Particle-Induced Erosion Wear on the Deflector Jet Servo Valve Prestage
Authors: Liang Na , Yuan Zhaohui , Zhang Fuli
Published: January 2023

Severe oil particle-induced erosion to the prestage component progressively degrades the overall performance of the deflector jet servo valve (DJSV), even leading to valve failure. Herein, we present an approach for evaluating degradation in performance and predicting the erosion lifespan of the DJSV on different levels of oil pollution. Specifically, a mathematical model of the whole valve was built based on a previously established working principle and physical mechanism. In addition, considering the horizontal and rotational particle motions, combined with impact of particle size distributions under different oil contamination degrees, an erosion model was constructed. Then, after simulating and analyzing the pressure characteristics before and after the erosion of prestage, the performance degradation of the whole valve was examined, thereby predicting the erosion life of the valve. Investigations revealed that the maximum erosion rate occurred at the shunt wedge of the receiving holes, which increased with the contamination degree and accelerated after level 7. After erosion, however, the control pressure difference decreased significantly, and erosion life followed exponential distribution corresponding to the distribution of particles under different pollution levels. The aforementioned investigation can thus help diagnose faults and optimize the design of the servo valves in service.
Mechanism and Empirical Test of the Impact of Consumption Upgrading on Agricultural Green Total Factor Productivity in China
Authors: Xing Xiaowei , Zhang Qingfeng , Ye Azhong , Zeng Guanghui
Published: January 2023

To explore the relationship between consumption upgrading and agricultural green total factor productivity in the context of green and high-quality development of agriculture in China. Based on the construction of a composite index of consumption upgrading and the Malmquist index of non-expected output in the SBM-DEA model to measure agricultural green total factor productivity, this paper uses the PVAR model and panel data from 30 Chinese provinces from 2008 to 2020 to empirically analyze the mechanism of the effect of consumption upgrading on agricultural green total factor productivity under high-quality development. The results are as follows: (1) Both the real economy and consumption upgrading are ahead of the change in agricultural green total factor productivity and have a negative short-run impact on agricultural green total factor productivity but a continuous boosting effect in the long-run. (2) In terms of specific impact paths, the real economy boosts agricultural green total factor productivity through technical efficiency and technical change paths and has a negative impact through scale efficiency, whereas consumption upgrading has inhibitory and sustained promotional effects in the short- and long-run, respectively, through technical efficiency and technical change paths and has opposite impact effects in the scale efficiency path.
A New Approach for Agricultural Water Management Using Pillows Made from COVID-19 Waste Face Masks and Filled with a Hydrogel Polymer: Preliminary Studies
Authors: Kolya Haradhan , Kang Chun-Won
Published: January 2023

Face masks have become an essential commodity during the COVID-19 pandemic, and their use rises daily. Excessive face mask use will likely continue to combat the virus and bacterial impacts in the long term. Afterward, used face masks are hazardous to the environment since most are made of nonbiodegradable porous polymeric fibrous materials. Thus, finding new ways to recycle waste face masks is urgently needed. Similarly, managing agricultural water for irrigation is a crucial challenge in saving water. This study demonstrates an approach for recycling face masks as bag- or small-sized pillows filled with superabsorbent polymers (SAPs) for the slow release of water near plant roots. Previous studies have reported that SAPs or hydrogel could boost soil’s water retention capacity, mixed with hydrogel/SAP. However, mixing SAPs into soil is improper because biodegradation generates low toxic organic molecules and contaminates soil and surface water. The objective of this research was to develop a face mask reuse approach, reduce irrigation water using polymers, and reduce toxic contamination in the soil. Here, swollen SAPs were taken inside the pillow and buried near plants, and the growth of the plants was studied. The moisture of the inner soil was constant for a long time, boosting plant growth. Afterward, the face mask pillows could be removed from the soil and maintained for further use. This new approach could be helpful in pot farming. This approach could contribute to the circular economy and the development of environmental sustainability.
Assessment of Biometric Parameters and Health of Canna’s Cultivars as Plant Useful in Phytoremediation of Degraded Agrocenoses
Authors: Szmagara Mariusz , Kopacki Marek , Skwaryło-Bednarz Barbara , Jamiołkowska Agnieszka , Marcinek Barbara , Rysiak Krystyna , Szmagara Agnieszka
Published: January 2023

Recently, the ecological awareness of society and the need to take care of our surroundings and the natural environment has significantly increased. There is also an urgent problem of searching for new, environmentally friendly techniques for its purification (soil, ground and surface waters, sewage sludge and air) with the use of living organisms, especially higher plants. One plant species investigated for phytoremediation is canna. Ten varieties of canna, grown on degraded and garden soil, were tested in this respect. The disease index and species composition of fungi inhabiting its organs, growth dynamics, parameters of photosynthesis and gas exchange were determined. The conducted research showed that cannas are able to satisfactorily grow even in seemingly unfavorable soil conditions with its strong degradation. Among a total of 24 species of fungi obtained from its organs, genus Fusarium, considered as pathogenic for canna, Alternaria alternata, and, less frequently, Thanatephorus cucumeris and Botrytis cinerea, dominated. The cultivars ‘Picasso’, ‘Cherry Red’, ‘President’ and ‘La Boheme’ had lower rates of photosynthesis and gas exchange than the least affected ‘Botanica’, ‘Wyoming’, ‘Robert Kemp’ and ‘Lucifer’ cultivars. Those turned out to be the most beneficial and they can be recommended for cultivation on strongly degenerated soils.
Design and Experimental Study on a New Horizontal Rotary Precision Seed Metering Device for Hybrid Rice
Authors: Xia Qianqian , Zhang Wenyi , Qi Bing , Wang Yunxia
Published: January 2023

In order to meet the sowing demand of the hybrid rice plug seedling, a horizontal rotary precision seed metering device was proposed. The key structure parameters were designed, the kinematics analysis of the seed-filling process was carried out, and the main influencing factors of the seeding performance evaluation index were determined. Taking motor speed, the eccentric distance of the disk, and seed layer thickness as test factors, and the taking qualification rate, repetition rate, and miss-seeding rate as seeding performance test indexes, the quadratic regression orthogonal rotation combination test was carried out, and the relationship between the test factors and evaluation indexes was approached by a quadratic polynomial. On the basis of discussing the influence of the test factors on the performance index, the regression surrogate mathematical model between the main performance index and the influencing factors was established. The value of the evaluation index of correlation degree p between the model variables was less than 0.01. Based on the regression surrogate mathematical model, the response surface of the qualified rate of the seeding performance evaluation index was established, and the test data were analyzed by multiple regression analysis. The rapid prediction of qualified rates under the influence of test factors was realized, and the optimum test parameters were obtained as follows: motor speed 165 r/min, an eccentric distance of disk 22 mm, seed layer thickness 28 mm, qualification rate 98.55%, miss-seeding rate 1.45%, and repetition rate 0%. According to the best combination of test parameters, the performance evaluation index test of the prototype was carried out, and the qualification rate, miss-seeding rate, and repetition rate were 98.34%, 1.66%, and 0%. The experimental results were in good agreement with the theoretical analysis, indicating that the horizontal rotary precision seed metering device can meet the agronomic requirements of hybrid rice. The research results could provide some reference for the design of a precision seeding device for industrialized plug seedlings.
Sustainable Utilization Strategy of Organic Waste via Fabrication of Bioelastomer with Antibacterial and Antioxidant Activities Using Mandarin Peel Extracts
Authors: Lee Kang , Chun Youngsang , Lee Ja , Lee Jong , Lee Taek , Yoo Hah
Published: January 2023

Mandarin peels (MPs), a food-processing residue, have several restrictions on their disposal and can cause serious environmental pollution. In this study, MP was used to fabricate a functional bioelastomer with antioxidant and antibacterial activities. Bioactive compounds were recovered from MPs in liquid form and added to the bioelastomer during fabrication to maintain the mechanical strength of the bioelastomer. The radical scavenging activities of the fabricated bioelastomer (B–MPE 15%) were 3.3% for DPPH and 20.8% for ABTS, respectively. In addition, B–MPE 15% exhibited antibacterial activity against gram-positive (Staphylococcus aureus), gram-negative (Escherichia coli), and antibiotic-resistant bacteria (Methicillin-resistant S. aureus and Vancomycin resistant Enterococcus). The chemical properties of B–MPE 15% were not significantly different from those of the control group (bare PDMS). Tensile strength, elongation at break, and water vapor transmission rate of B–MPE 15% were found to be 5.1 N/mm2, 649%, and 33.3 g/(m2 day), respectively. Therefore, the addition of MP extracts did not significantly affect the physical properties. The fabricated bioelastomer with antibacterial and antioxidant activities is expected to be utilized in the food packaging, pharmaceutical, and medical industries. Our research is expected to represent a future-oriented strategy for realizing carbon neutrality by upcycling food waste.
Population Structure and Genetic Diversity of Colletotrichum gloeosporioides on Citrus in China
Authors: Liu Bei , Liang Xingxing , Kong Jinchao , Jiao Chen , Li Hongye , Gai Yunpeng
Published: January 2023

To analyze the genetic structure and genetic diversity of Colletotrichum gloeosporioides as the dominant Colletotrichum species on Citrus, the Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genetic diversity, including 63 strains isolated and selected from 8 different sites and 5 different citrus species, was studied. A total of 19 GAPDH haplotypes were identified by genetic analysis, and the main haplotype (haplotype 5) was distributed in 28 isolates, mainly from Citrus unchiu Hort. ex Tanaka (WG) and Citrus reticulata Blanco cv. Succosa (BDZ) in Huangyan (HY), Linhai (LH), and Jiande (JD) of Zhejiang province, and Mashui tangerine (MSJ) in Mengshan of Guangxi province (GX). Using the genetic differentiation index, Fst revealed significant genetic differentiation in C. gloeosporioides populations between Jiangxi province (JXGZ) and GX, HY, LH, JD, and Chun’an (CA) of Zhejiang province, and also revealed slightly less genetic differentiation for C. gloeosporioides populations between HY, LH, JD, GX, Shaanxi province (SX), and Quzhou (QZ) of Zhejiang province. In addition, Fst revealed great genetic differentiation between the C. gloeosporioides populations obtained from MSJ and Citrus paradise Macf (PTY), and also revealed weak genetic differentiation between the C. gloeosporioides populations obtained from Citrus sinensis Osbeck (QC), WG, and BDZ. The AMOVA test showed that the levels of genetic differentiation for C. gloeosporioides were 19% and 81% among and within geographic populations, respectively. It also showed that C. gloeosporioides had levels of genetic differentiation among and within host populations of 12% and 88%, respectively. The Mantel test showed that the genetic distance was not linearly correlated with geographical distance and the haplotype phylogenetic analysis showed that C. gloeosporioides from different regions and hosts were scattered in the phylogenetic tree, implying that the genetic differentiation was independent of host variety and geographical origin. We speculated that genetic differentiation may be mainly due to gene mutation, gene recombination, or gene migration within native populations and has nothing to do with natural selection triggered by geography or host variety.
Thymol Induces Cell Death of Fusarium oxysporum f. sp. niveum via Triggering Superoxide Radical Accumulation and Oxidative Injury In Vitro
Authors: Hao Yini , Zhang Jiao , Sun Changwei , Chen Xuenai , Wang Yuxiao , Lu Haiyan , Chen Jian , Shi Zhiqi , Zhang Li , Yang Lifei , Huang Sijie
Published: January 2023

Fusarium oxysporum f. sp. niveum (FON) causes watermelon wilt that is one of the major disease-causing yield losses of watermelon. Sustainable development of agriculture requires controlling watermelon wilt disease with good environmental performance. One important approach is to identify environmental-friendly compounds with inhibitory activity against FON. Thymol is a plant-derived compound that is safe for ecology. Little is known about the application of thymol in agriculture. In this study, we studied the inhibitory activity of thymol against FON by using morphological, physiological, and histochemical approaches. Thymol significantly inhibited colony diameter of FON in a dose-dependent manner, with EC50 at 21 µg/mL. Thymol at 10, 21, and 35 µg/mL decreased the fresh weight of FON mycelia by 29.0%, 50.6%, and 69.5%, respectively. Microscopic observation revealed irregular damage and loss of shape of mycelia upon thymol exposure. Thymol induced the accumulation of superoxide radical in mycelial cells and accompanied increased activity of antioxidative enzymes (SOD, superoxide dismutase; CAT, catalase). Thymol induced membrane permeability was indicated by lipid peroxidation and electrolyte leakage (increased by 29–58%) in mycelial cells. These results suggested that thymol induced oxidative damage in mycelia, which may be one of the possible reasons for thymol-induced mycelial cell death observed with fluorescent detection. Thymol decreased the production of conidia and inhibited the germination of conidia. Thymol induced superoxide radical accumulation, lipid peroxidation, and cell death in conidia as well. All of these results revealed the inhibitory activity of thymol against FON, which may have resulted from the superoxide radical-induced oxidative injury in both conidia and mycelia of FON.
Foliar Applications of ZnO and Its Nanoparticles Increase Safflower (Carthamus tinctorius L.) Growth and Yield under Water Stress
Authors: Ghiyasi Mahdi , Rezaee Danesh Younes , Amirnia Reza , Najafi Solmaz , Mulet José M. , Porcel Rosa
Published: January 2023

Foliar application or minerals is a methodology to promote growth and/or yield and to protect plants against different kinds of stresses. Currently there is a great interest in evaluating the effect of nanoparticles for enhancing the effect of these treatments. This study was performed to evaluate and compare the effect of foliar application of zinc oxide (ZnO) and zinc oxide nanoparticles (ZnO-NPs) on the growth and yield of safflower under different irrigation regimes. Foliar applications of ZnO in all concentrations (4, 6, 8, 10, 12, and 14 g L−1) led to an increase in biomass yield, number of capitula per plant, number of seeds per capitulum, and grain yield of plants compared with control plants. The maximum increase in the studied traits was obtained with a ZnO concentration of 6, 8, and 10 g L−1. In a second round of experiments, we observed the effect of nanoparticles and found that spraying with ZnO and ZnO-NPs at a concentration of 10 g L−1 may ameliorate the deleterious effects of water deficit. The results of the present study support the idea that foliar application of ZnO improves safflower yield, especially under drought stress, and showed that using of nanoparticles increases the efficiency of the application.
Effect of Sugar Beet Pulp and Anionic Salts on Metabolic Status and Mineral Homeostasis during the Peri-Parturient Period of Dairy Sheep
Authors: Peleki Christina , Kiosis Evangelos , Polizopoulou Zoe S. , Tsousis Georgios , Fthenakis George C. , Giadinis Nektarios D. , Brozos Christos
Published: January 2023

Sugar beet pulp is a popular by-product of sugar extraction; however, it can potentially cause depletion of Ca availability due to its oxalic content. The experiment examined the effect of sugar beet pulp and anionic salts administration during the dry period on the serum concentration of calcium, magnesium, phosphate, and potassium of dairy sheep. Eighty-seven sheep were divided into three groups (A, B, and C) according to their body condition score (BCS) and age at 40 days before the expected lambing. All groups received alfalfa hay, mixed grass straw, and a concentrate supplement. The concentrate fed to groups B and C contained sugar beet pulp. The nutritional value fed to all three groups was similar, except for Dietary Cation Anion Difference (DCAD). Animals of group A had a DCAD of +198 mEq/kg, animals of group B of +188 mEq/kg, and animals of group C were fed 20 gr/d ammonium chloride to achieve a negative DCAD (−52 mEq/kg). All groups were fed the same ration after lambing. Blood samples were collected 30 d, 20 d, 17 d, 14 d, 10 d, 7 d, and 4 d before lambing (a.p.), 6 h, 12 h, 24 h, 7 d, 10 d, and 15 d after lambing (p.p) for calcium, magnesium, phosphate, and potassium, and 30 d a.p., 7 d, and 15 d p.p. for beta hydroxybutyrate acid (BHBA) concentrations. Urine samples were also collected 20 d, 10 d, 4 d a.p., and 7 d p.p for the evaluation of pH levels. Ca levels of the control group decreased earlier and were lower at 4 d a.p. compared to those of group B and C. Additionally, the control group showed lower p values compared to group C at 20 d and 17 d a.p. P levels recovered earlier post parturition in young (age 1–1.5 years old) compared to older ewes. Group C had lower urine pH values throughout the pre-parturient period, reflecting the acidifying effect of the administered ammonium chloride, without any side effect on macromineral blood concentration. Feeding sugar beet pulp and systemic acidifying before parturition is considered safe and might even be beneficial in preventing hypocalcemia.
Sympathetic Arousal Detection in Horses Using Electrodermal Activity
Authors: Golzari Kia , Kong Youngsun , Reed Sarah A. , Posada-Quintero Hugo F.
Published: January 2023

The continuous monitoring of stress, pain, and discomfort is key to providing a good quality of life for horses. The available tools based on observation are subjective and do not allow continuous monitoring. Given the link between emotions and sympathetic autonomic arousal, heart rate and heart rate variability are widely used for the non-invasive assessment of stress and pain in humans and horses. However, recent advances in pain and stress monitoring are increasingly using electrodermal activity (EDA), as it is a more sensitive and specific measure of sympathetic arousal than heart rate variability. In this study, for the first time, we have collected EDA signals from horses and tested the feasibility of the technique for the assessment of sympathetic arousal. Fifteen horses (six geldings, nine mares, aged 13.11 ± 5.4 years) underwent a long-lasting stimulus (Feeding test) and a short-lasting stimulus (umbrella Startle test) to elicit sympathetic arousal. The protocol was approved by the University of Connecticut. We found that EDA was sensitive to both stimuli. Our results show that EDA can capture sympathetic activation in horses and is a promising tool for non-invasive continuous monitoring of stress, pain, and discomfort in horses.
Acute Kidney Injury Is Associated with Higher Serum Cys-C and NGAL Concentrations, and Risk of Mortality in Premature Calves with Respiratory Distress Syndrome
Authors: Ider Merve , Ok Mahmut , Naseri Amir , Erturk Alper , Parlak Tugba Melike , Yildiz Ramazan , Durgut Murat Kaan
Published: January 2023

The purpose of the present study was to establish the development of acute kidney injury (AKI) and evaluate the usefulness of kidney-specific biomarkers in diagnosing AKI in premature calves with respiratory distress syndrome (RDS). Ten-term healthy and 70 premature calves with RDS were enrolled. Clinical examination, blood gases, and chemical analysis were performed at admission and 72 h. Serum concentrations of blood urea nitrogen (BUN), creatinine (Cre), phosphorus (P), cystatin-C (Cys-C), neutrophil gelatinase-associated lipocalin (NGAL), uromodulin (UMOD), and liver-type fatty acid-binding protein (L-FABP) were measured to evaluate kidney injury. Our findings showed that 38.5% of the premature calves with RDS developed AKI. The RDS-AKI group had a 4-fold higher mortality risk than the RDS-non-AKI group. Cys-C, with 90% and 89% specificity, and NGAL, with 100% sensitivity and 85% specificity, were the most reliable biomarkers to determine AKI in premature calves. The usefulness of any biomarker to predict mortality was not found to be convincing. In conclusion, AKI can develop as a consequence of hypoxia in premature calves and may increase the risk of mortality. In addition, serum Cys-C and NGAL concentrations may be useful in the diagnosis of AKI in premature calves with RDS.
TLR7 and IgM: Dangerous Partners in Autoimmunity
Authors: Amendt Timm , Yu Philipp
Published: January 2023

The B cell antigen receptor (BCR)-repertoire is capable of recognizing a nearly unlimited number of antigens. Inevitably, the random nature of antibody gene segment rearrangement, needed in order to provide mature B cells, will generate autoreactive specificities. Once tolerance mechanisms fail to block the activation and differentiation of autoreactive B cells, harmful autoantibodies may get secreted establishing autoimmune diseases. Besides the hallmark of autoimmunity, namely IgG autoantibodies, IgM autoantibodies are also found in many autoimmune diseases. In addition to pathogenic functions of secreted IgM the IgM-BCR expressing B cell might be the initial check-point where, in conjunction with innate receptor signals, B cell mediated autoimmunity starts it fateful course. Recently, pentameric IgM autoantibodies have been shown to contribute significantly to the pathogenesis of various autoimmune diseases, such as rheumatoid arthritis (RA), autoimmune hemolytic anemia (AIHA), pemphigus or autoimmune neuropathy. Further, recent studies suggest differences in the recognition of autoantigen by IgG and IgM autoantibodies, or propose a central role of anti-ACE2-IgM autoantibodies in severe COVID-19. However, exact mechanisms still remain to be uncovered in detail. This article focuses on summarizing recent findings regarding the importance of autoreactive IgM in establishing autoimmune diseases.
Carbapenemase-Producing Klebsiella pneumoniae in COVID-19 Intensive Care Patients: Identification of IncL-VIM-1 Plasmid in Previously Non-Predominant Sequence Types
Authors: Cañada-García Javier E. , Ramírez de Arellano Eva , Jiménez-Orellana Miguel , Viedma Esther , Sánchez Aida , Alhambra Almudena , Villa Jennifer , Delgado-Iribarren Alberto , Bautista Verónica , Lara Noelia , García-Cobos Silvia , Aracil Belén , Cercenado Emilia , Pérez-Vázquez María , Oteo-Iglesias Jesús
Published: January 2023

During the COVID-19 pandemic, intensive care units (ICUs) operated at or above capacity, and the number of ICU patients coinfected by nosocomial microorganisms increased. Here, we characterize the population structure and resistance mechanisms of carbapenemase-producing Klebsiella pneumoniae (CP-Kpn) from COVID-19 ICU patients and compare them to pre-pandemic populations of CP-Kpn. We analyzed 84 CP-Kpn isolates obtained during the pandemic and 74 CP-Kpn isolates obtained during the pre-pandemic period (2019) by whole genome sequencing, core genome multilocus sequence typing, plasmid reconstruction, and antibiotic susceptibility tests. More CP-Kpn COVID-19 isolates produced OXA-48 (60/84, 71.4%) and VIM-1 (18/84, 21.4%) than KPC (8/84, 9.5%). Fewer pre-pandemic CP-Kpn isolates produced VIM-1 (7/74, 9.5%). Cefiderocol (97.3–100%) and plazomicin (97.5–100%) had the highest antibiotic activity against pandemic and pre-pandemic isolates. Sequence type 307 (ST307) was the most widely distributed ST in both groups. VIM-1-producing isolates belonging to ST307, ST17, ST321 and ST485, (STs infrequently associated to VIM-1) were detected during the COVID-19 period. Class 1 integron Int1-blaVIM-1-aac(6′)-1b-dfrB1-aadAI-catB2-qacEΔ1/sul1, found on an IncL plasmid of approximately 70,000 bp, carried blaVIM-1 in ST307, ST17, ST485, and ST321 isolates. Thus, CP-Kpn populations from pandemic and pre-pandemic periods have similarities. However, VIM-1 isolates associated with atypical STs increased during the pandemic, which warrants additional monitoring and surveillance.
Development and Implementation of a Mobile Application for Choosing Empirical Antimicrobial Therapy for Bacteremia, Pneumonia, Urinary Tract Infection, and Skin and Soft Tissue Infection among Hospitalized Patients
Authors: Chaloernpoj Kanthon , Wangchinda Walaiporn , Koomanachai Pornpan , Thamlikitkul Visanu , Rattanaumpawan Pinyo
Published: January 2023

Clinical practice guidelines (CPGs) and computerized clinical decision support programs are effective antimicrobial stewardship strategies. The DigitalAMS™, a mobile-based application for choosing empirical antimicrobial therapy under the hospital’s CPGs, was implemented at Siriraj Hospital and evaluated. From January to June 2018, a cross-sectional study was conducted among 401 hospitalized adults who received ≥1 dose of antimicrobials and had ≥1 documented site-specific infection. The antimicrobial regimen prescribed by the ward physician (WARD regimen), recommended by the DigitalAMS™ (APP regimen), and recommended by two independent infectious disease (ID) physicians before (Emp-ID regimen) and after (Def-ID regimen) the final microbiological results became available were compared in a pairwise fashion. The percent agreement of antimicrobial prescribing between the APP and Emp-ID regimens was 85.7% in the bacteremia group, 59.1% in the pneumonia group, 78.6% in the UTI group, and 85.2% in the SSTI group. The percent agreement between the APP and Emp-ID regimens was significantly higher than that between the WARD and Emp-ID regimens in three site-specific infection groups: the bacteremia group (85.7% vs. 47.9%, p < 0.001), the UTI group (78.6% vs. 37.8%, p < 0.001), and the SSTI group (85.2% vs. 40.2%, p < 0.001). Furthermore, the percent agreement between the APP and Def-ID regimens was similar to that between the Emp-ID and Def-ID regimens in all sites of infection. In conclusions, the implementation of DigitalAMS™ seems useful but needs some revisions. The dissemination of this ready-to-use application with customized clinical practice guidelines to other hospital settings may be beneficial.
Local Antibiotics in the Treatment of Diabetic Foot Infections: A Narrative Review
Authors: Soldevila-Boixader Laura , Fernández Alberto Pérez , Laguna Javier Muñoz , Uçkay Ilker
Published: January 2023

Along with the increasing global burden of diabetes, diabetic foot infections (DFI) and diabetic foot osteomyelitis (DFO) remain major challenges for patients and society. Despite progress in the development of prominent international guidelines, the optimal medical treatment for DFI and DFO remains unclear as to whether local antibiotics, that is, topical agents and local delivery systems, should be used alone or concomitant to conventional systemic antibiotics. To better inform clinicians in this evolving field, we performed a narrative review and summarized key relevant observational studies and clinical trials of non-prophylactic local antibiotics for the treatment of DFI and DFO, both alone and in combination with systemic antibiotics. We searched PubMed for studies published between January 2000 and October 2022, identified 388 potentially eligible records, and included 19 studies. Our findings highlight that evidence for adding local antibiotic delivery systems to standard DFO treatment remains limited. Furthermore, we found that so far, local antibiotic interventions have mainly targeted forefoot DFO, although there is marked variation in the design of the included studies. Suggestive evidence emerging from observational studies underscores that the addition of local agents to conventional systemic antibiotics might help to shorten the clinical healing time and overall recovery rates in infected diabetic foot ulcers, although the effectiveness of local antibiotics as a standalone approach remains overlooked. In conclusion, despite the heterogeneous body of evidence, the possibility that the addition of local antibiotics to conventional systemic treatment may improve outcomes in DFI and DFO cannot be ruled out. Antibiotic stewardship principles call for further research to elucidate the potential benefits of local antibiotics alone and in combination with conventional systemic antibiotics for the treatment of DFI and DFO.
Use of High-Dose Nebulized Colistimethate in Patients with Colistin-Only Susceptible Acinetobacter baumannii VAP: Clinical, Pharmacokinetic and Microbiome Features
Authors: De Pascale Gennaro , Pintaudi Gabriele , Lisi Lucia , De Maio Flavio , Cutuli Salvatore Lucio , Tanzarella Eloisa Sofia , Carelli Simone , Lombardi Gianmarco , Cesarano Melania , Gennenzi Veronica , Ciotti Gabriella Maria Pia , Grieco Domenico Luca , Posteraro Brunella , Sanguinetti Maurizio , Navarra Pierluigi , Antonelli Massimo
Published: January 2023

(1) Background: Colistin-only susceptible (COS) Acinetobacter baumannii (AB) ventilator-associated pneumonia (VAP) represents a clinical challenge in the Intensive Care Unit (ICU) due to the negligible lung diffusion of this molecule and the low-grade evidence on efficacy of its nebulization. (2) Methods: We conducted a prospective observational study on 134 ICU patients with COS-AB VAP to describe the ‘real life’ clinical use of high-dose (5 MIU q8) aerosolized colistin, using a vibrating mesh nebulizer. Lung pharmacokinetics and microbiome features were investigated. (3) Results: Patients were enrolled during the COVID-19 pandemic with the ICU presenting a SAPS II of 42 [32–57]. At VAP diagnosis, the median PaO2/FiO2 was 120 [100–164], 40.3% were in septic shock, and 24.6% had secondary bacteremia. The twenty-eight day mortality was 50.7% with 60.4% and 40.3% rates of clinical cure and microbiological eradication, respectively. We did not observe any drug-related adverse events. Epithelial lining fluid colistin concentrations were far above the CRAB minimal-inhibitory concentration and the duration of nebulized therapy was an independent predictor of microbiological eradication (12 [9.75–14] vs. 7 [4–13] days, OR (95% CI): 1.069 (1.003–1.138), p = 0.039). (4) Conclusions: High-dose and prolonged colistin nebulization, using a vibrating mesh, was a safe adjunctive therapeutic strategy for COS-AB VAP. Its right place and efficacy in this setting warrant investigation in interventional studies.
Sphingolipids and Atherosclerosis: The Dual Role of Ceramide and Sphingosine-1-Phosphate
Authors: Piccoli Marco , Cirillo Federica , Ghiroldi Andrea , Rota Paola , Coviello Simona , Tarantino Adriana , La Rocca Paolo , Lavota Ivana , Creo Pasquale , Signorelli Paola , Pappone Carlo , Anastasia Luigi
Published: January 2023

Sphingolipids are bioactive molecules that play either pro- and anti-atherogenic roles in the formation and maturation of atherosclerotic plaques. Among SLs, ceramide and sphingosine-1-phosphate showed antithetic properties in regulating various molecular mechanisms and have emerged as novel potential targets for regulating the development of atherosclerosis. In particular, maintaining the balance of the so-called ceramide/S1P rheostat is important to prevent the occurrence of endothelial dysfunction, which is the trigger for the entire atherosclerotic process and is strongly associated with increased oxidative stress. In addition, these two sphingolipids, together with many other sphingolipid mediators, are directly involved in the progression of atherogenesis and the formation of atherosclerotic plaques by promoting the oxidation of low-density lipoproteins (LDL) and influencing the vascular smooth muscle cell phenotype. The modulation of ceramide and S1P levels may therefore allow the development of new antioxidant therapies that can prevent or at least impair the onset of atherogenesis, which would ultimately improve the quality of life of patients with coronary artery disease and significantly reduce their mortality.
Dietary Protein Levels Modulate the Antioxidant Capacity during Different Growth Stages in Huanjiang Mini-Pigs
Authors: Liu Yating , Azad Md. Abul Kalam , Zhao Xichen , Zhu Qian , Kong Xiangfeng
Published: January 2023

Adequate crude protein (CP) levels in diets play potential roles in swine production. This study determined the impacts of different CP levels on the antioxidant capacity of pigs during different body weight (BW) stages. Three hundred and sixty Huanjiang mini-pigs were allocated to one of three independent experiments, including a 5–10 kg BW group, where CP levels included 14%, 16%, 18%, 20%, and 22%; a 10–20 kg BW group, where CP levels included 12%, 14%, 16%, 18%, and 20%; and a 20–30 kg BW group, where CP levels included 10%, 12%, 14%, 16%, and 18%. These independent experiments were conducted for 28, 28, and 26 days, respectively. Results showed that the 20% CP level increased (p < 0.05) the plasma CAT and GSH-Px activities and the GSH concentration of pigs than in the pigs supplemented with the 14–18% CP levels, and the 20% CP level up-regulated (p < 0.05) the ileal oxidative stress-related gene expression levels of pigs than in the pigs supplemented with the 14% CP level at the 5–10 kg BW. In addition, diets supplemented with 18% CP level increased (p < 0.05) the ileal GSH concentration of pigs than in the pigs supplemented with the 14% and 20% CP levels, and the 16–18% CP levels increased (p < 0.05) the jejunal SOD activity of pigs than in the pigs supplemented with the 14% CP level. At 10–20 kg BW, the 16% CP level presented the strongest jejunal and ileal antioxidant capacity, the 18% CP level had the lowest plasma concentrations of MDA and highest GSH, and the 14–16% CP levels increased the plasma CAT and SOD activities (p < 0.05). Moreover, the 16–20% CP levels up-regulated (p < 0.05) the oxidative stress-related gene expression levels. At 20–30 kg BW, diets supplemented with the 16% CP level increased the plasma CAT activity of pigs than in the pigs supplemented with the 12–14% CP levels, and the 14–16% CP levels decreased the MDA concentration compared with the 10% CP levels (p < 0.05). In conclusion, these findings indicate adequate CP levels of 20%, 16%, and 14% for Huanjiang mini-pigs at the 5–10, 10–20, and 20–30 kg BW stages, respectively.
Kinin B1 Receptor Mediates Bidirectional Interaction between Neuroinflammation and Oxidative Stress
Authors: Theobald Drew , Sriramula Srinivas
Published: January 2023

Hypertension is associated with increased expression of kinin B1 receptors (B1R) and increased levels of pro-inflammatory cytokines within the neurons. We previously reported that angiotensin II (Ang II) upregulates B1R expression and can induce neuroinflammation and oxidative stress in primary hypothalamic neurons. However, the order in which B1R activation, neuroinflammation, and oxidative stress occur has not yet been studied. Using primary hypothalamic neurons from neonatal mice, we show that tumor necrosis factor (TNF), lipopolysaccharides (LPS), and hydrogen peroxide (H2O2) can upregulate B1R expression and increase oxidative stress. Furthermore, our study shows that B1R blockade with R715, a specific B1R antagonist, can attenuate these effects. To further confirm our findings, we used a deoxycorticosterone acetate (DOCA)-salt model of hypertension to show that oxidative stress is upregulated in the hypothalamic paraventricular nucleus (PVN) of the brain. Together, these data provide novel evidence that relationship between oxidative stress, neuroinflammation, and B1R upregulation in the brain is bidirectional, and that B1R antagonism may have beneficial effects on neuroinflammation and oxidative stress in various disease pathologies.
Quantitative Proteomics Identifies Novel Nrf2-Mediated Adaptative Signaling Pathways in Skeletal Muscle Following Exercise Training
Authors: Bhat Anjali , Abu Rafay , Jagadesan Sankarasubramanian , Vellichirammal Neetha Nanoth , Pendyala Ved Vasishtha , Yu Li , Rudebush Tara L. , Guda Chittibabu , Zucker Irving H. , Kumar Vikas , Gao Lie
Published: January 2023

Exercise training (ExT) improves skeletal muscle health via multiple adaptative pathways. Nrf2 is a principal antioxidant transcription factor responsible for maintaining intracellular redox homeostasis. In this study, we hypothesized that Nrf2 is essential for adaptative responses to ExT and thus beneficial for muscle. Experiments were carried out on male wild type (WT) and iMS-Nrf2flox/flox inducible muscle-specific Nrf2 (KO) mice, which were randomly assigned to serve as sedentary controls (Sed) or underwent 3 weeks of treadmill ExT thus generating four groups: WT-Sed, WT-ExT, KO-Sed, and KO-ExT groups. Mice were examined for exercise performance and in situ tibialis anterior (TA) contractility, followed by mass spectrometry-based proteomics and bioinformatics to identify differentially expressed proteins and signaling pathways. We found that maximal running distance was significantly longer in the WT-ExT group compared to the WT-Sed group, whereas this capacity was impaired in KO-ExT mice. Force generation and fatigue tolerance of the TA were enhanced in WT-ExT, but reduced in KO-ExT, compared to Sed controls. Proteomic analysis further revealed that ExT upregulated 576 proteins in WT but downregulated 207 proteins in KO mice. These proteins represent pathways in redox homeostasis, mitochondrial respiration, and proteomic adaptation of muscle to ExT. In summary, our data suggest a critical role of Nrf2 in the beneficial effects of SkM and adaptation to ExT.
A Low-Voltage AC, Low-Voltage DC, and High-Voltage DC Power Distribution System with Grid: Design and Analysis
Authors: Zdiri Mohamed Ali , Dhouib Bilel , Alaas Zuhair , Hadj Abdallah Hsan
Published: January 2023

Low-voltage (LV) and high-voltage (HV) DC distribution systems are being investigated as alternatives due to the growth of DC distribution energy resources (DER), DC loads such as solar and wind power systems, and energy storage sources (ESSs). Furthermore, an HV/LV DC distribution system offers various advantages, including lower conversion losses, an easier connecting strategy for DC DERs, and less complex power management techniques. As renewable energy sources are increasingly incorporated into the electrical grid, it is important to create novel, effective approaches for connecting such sources and loads. It would hence be effective to merge DC distribution with AC distribution to fulfill the energy demands of both DC and AC consumers. To this end, this study proposes a multizone design with four buses: low-voltage direct current (LVDC), high-voltage direct current (HVDC), low-voltage alternating current (LVAC), and an electrical grid. A model of this system that covers crucial elements, including power systems, DER systems, and power electronic devices, to serve as a foundation for the analysis and design of this architecture is proposed. MATLAB/Simulink is used to conduct a simulation study to verify the performance of the proposed design. In this study, a hybrid electrical grid with an LVDC, HVDC, and LVAC distribution network test is used and implemented. Additionally, a transient and steady-state characteristic analysis of the test system is performed.
Morphological, Functional and Texture Analysis Magnetic Resonance Imaging Features in the Assessment of Radiotherapy-Induced Xerostomia in Oropharyngeal Cancer
Authors: Calamandrei Leonardo , Mariotti Luca , Bicci Eleonora , Calistri Linda , Barcali Eleonora , Orlandi Martina , Landini Nicholas , Mungai Francesco , Bonasera Luigi , Bonomo Pierluigi , Desideri Isacco , Bocchi Leonardo , Nardi Cosimo
Published: January 2023

The aim of this single-center, observational, retrospective study was to investigate magnetic resonance imaging (MRI) biomarkers for the assessment of radiotherapy (RT)-induced xerostomia. Twenty-seven patients who underwent radiation therapy for oropharyngeal cancer were divided into three groups according to the severity of their xerostomia—mild, moderate, and severe—clinically confirmed with the Common Terminology Criteria for Adverse Events (CTCAE). No severe xerostomia was found. Conventional and functional MRI (perfusion- and diffusion- weighted imaging) performed both pre- and post-RT were studied for signal intensity, mean apparent diffusion coefficient (ADC) values, k-trans, and area under the perfusion curves. Contrast-enhanced T1 images and ADC maps were imported into 3D slicer software, and salivary gland volumes were segmented. A total of 107 texture features were derived. T-Student and Wilcoxon signed-rank tests were performed on functional MRI parameters and texture analysis features to identify the differences between pre- and post-RT populations. A p-value < 0.01 was defined as acceptable. Receiver operating characteristic (ROC) curves were plotted for significant parameters to discriminate the severity of xerostomia in the pre-RT population. Conventional and functional MRI did not yield statistically significant results; on the contrary, five texture features showed significant variation between pre- and post-RT on the ADC maps, of which only informational measure of correlation 1 (IMC 1) was able to discriminate the severity of RT-induced xerostomia in the pre-RT population (area under the curve (AUC) > 0.7). Values lower than the cut-off of −1.473 × 10−11 were associated with moderate xerostomia, enabling the differentiation of mild xerostomia from moderate xerostomia with a 73% sensitivity, 75% specificity, and 75% diagnostic accuracy. Therefore, the texture feature IMC 1 on the ADC maps allowed the distinction between different degrees of severity of RT-induced xerostomia in the pre-RT population. Accordingly, texture analysis on ADC maps should be considered a useful tool to evaluate salivary gland radiosensitivity and help identify patients at risk of developing more serious xerostomia before radiation therapy is administered.
TRAL: A Tag-Aware Recommendation Algorithm Based on Attention Learning
Authors: Zuo Yi , Liu Shengzong , Zhou Yun , Liu Huanhua
Published: January 2023

A social tagging system improves recommendation performance by introducing tags as auxiliary information. These tags are text descriptions of target items provided by individual users, which can be arbitrary words or phrases, so they can provide more abundant information about user interests and item characteristics. However, there are many problems to be solved in tag information, such as data sparsity, ambiguity, and redundancy. In addition, it is difficult to capture multi-aspect user interests and item characteristics from these tags, which is essential to the recommendation performance. In the view of these situations, we propose a tag-aware recommendation model based on attention learning, which can capture diverse tag-based potential features for users and items. The proposed model adopts the embedding method to produce dense tag-based feature vectors for each user and each item. To compress these vectors into a fixed-length feature vector, we construct an attention pooling layer that can automatically allocate different weights to different features according to their importance. We concatenate the feature vectors of users and items as the input of a multi-layer fully connected network to learn non-linear high-level interaction features. In addition, a generalized linear model is also conducted to extract low-level interaction features. By integrating these features of different types, the proposed model can provide more accurate recommendations. We establish extensive experiments on two real-world datasets to validate the effect of the proposed model. Comparable results show that our model perform better than several state-of-the-art tag-aware recommendation methods in terms of HR and NDCG metrics. Further ablation studies also demonstrate the effectiveness of attention learning.