bluuebunny/crossref_metadata_2025 huggingface dataset:
Hubungan Sikap Kesehatan Gigi dan Mulut Penderita Terhadap Kepatuhan dalam Menjalani Perawatan Berulang
Authors: Hendani Rizkina , Prasetyo Edhie Arif , Sampoerno Galih
Published: July 2020

Background: Caries is the world's number one chronic disease and the prevalence increases in modern times. But most people ignore it and choice dental care only when it feels pain. It will worsen the condition of the tooth until require multivisit treatments. The number of visits required for multivisit treatments results incomplete treatment due to patient’s non-obedience. One of the factors that affect obedience is attitude. One's attitude towards something will determine someone's behavior towards the same thing. Purpose: To determine the relationship of patient’s dental and oral health attitude towards patient’s obedience during multivisit treatment. Method: Analytical observational research with cross-sectional study conducted on 30 patients who came to RSGMP UPF Conservative Dentistry FKG UNAIR Surabaya period September-November 2011. The data about the attitude is obtained from questionnaire, while collecting cards of patient’s status and direct interviews of the operators conducted to obtain data about patient’s obedience. Result: Based on the results of Spearman Correlation with α=0,05 obtained p-value=0,364 (p>0,05). It suggests that there is no significant relationship between patient’s dental and oral health attitude towards patient’s obedience during multivisit treatment. In addition, test results of the relationship between unexamined variables with obedience obtained p-value=0,010 (motivation), p=0,017 (desire), p=0,004 (perception), and p=0,009 (financing). This suggests that there is a significant relationship between motivation, desires, perceptions, and financing with obedience. Conclusion: There is no relationship between patient’s dental and oral health attitude towards patient’s obedience during multivisit treatment. However, other factors found associated, namely motivation, desires, perceptions, and financing.
Daya Hambat Aktivitas Enzim Glukosiltransferase (Gtf) Streptococcus mutans Oleh Ekstrak Temulawak (Curcuma Xanthorrhiza Roxb.)
Authors: Amanda A. , Kunarti Sri , Subiwahjudi Agus
Published: July 2020

Background: Streptococcus mutans is a bacteria which has glucosyltransferase (GTF) enzyme and acts as the main agent that causes dental caries. GTF enzyme will convert sucrose into fructose and glucan. Temulawak (Curcuma xanthorrhiza Roxb.) is one of the traditional herbs which has xanthorrhizol, curcumin, flavonoid, tanin, and saponin as an antibacterial agent. Purpose: The purpose of this research is to investigate the effect of temulawak extract (Curcuma xanthorrhiza Roxb.) to the activity of GTF enzyme Streptococcus mutans. Method: This research used 25%, 37,5%, and 50% concentration of  temulawak extract as the treatment, and 0.12% chlorhexidine gluconate as a control. The method of this research consists of three steps; preparing the temulawak extract concentration of 25%, 37,5%, and 50%, preparing the GTF enzyme from the supernatant of Streptococcus mutans, and testing GTF enzyme activity by analyzing the fructose concentration using High Performance Liquid Chromatography (HPLC). Perusal of the fructose area was based on the retention time of fructose. One unit of GTF enzyme activity is defined as the 1 μmol fructose / ml of enzyme / hour. Result: The obtained data then were analyzed by Post-Hoc Tukey (HSD). The result showed a significant difference between each treatment group with the control group (p<0.05). Conclussion: This research concludes that temulawak extract with 25%, 37,5%, and 50% concentrationcan’t inhibit the GTF enzyme activity of Streptococcus mutans.
Kemampuan hambat ekstrak temulawak (Curcuma xanthorrhiza Roxb.) terhadap adhesi bakteri Streptococcus mutans
Authors: B Erin Imaniar , Kunarti Sri , Saraswati Widya
Published: July 2020

Background : Streptococcus mutans is the most cariogenic microorganism in the oral cavity. Streptococcus mutans has the ability to use dietary carbohydrates, such as sucrose, to synthesize extracellular polysaccharides (glucan and fructan) through glucosyltransferase and fructosyltransferase. Extracellular polysaccharide has a role in promoting the adhesion of bacteria. Chlorhexidine  is the gold standard as antiplaque agent but chlorhexidine is not fully able to inhibit the adhesion of bacteria because it has an effect that can increase gtfC and gtfD expression on S. mutans in planktonic form. Temulawak extract (Curcuma xanthorrhiza Roxb.) contains of curcumin, xanthorrhizol, saponin, flavonoid and tannin that have an effect to inhibit activity and secretion of glucosyltransferase and fructosyltransferase. Purpose: The aim of this study is to find out the ability of temulawak extract (Curcuma xanthorrhiza Roxb.) to inhibit adhesion of Streptococcus mutans.  Method: This study was designed as an experimental laboratory study with post test only control group design using Streptococcus mutans. Temulawak was extracted using maceration method. The number of bacteria that can perform adhesion to the tooth surface can be determined by performing calculations using formulas plate count method. Result: Temulawak extract (Curcuma xanthorrhiza Roxb.) at concentrations of 25%, 37.5%, 50% and chlorhexidine can inhibit bacterial adhesion of Streptococcus mutans. Conclusion: Temulawak extract at concentration of 50% and 37.5% has the best ability to inhibit the adhesion of bacteria among other treatment groups.
Perbedaan Perlekatan Biofilm Streptococcus mutans pada Resin Komposit Nanofil Tipe Universal Restortive dan Flowable Restorative
Authors: Kurniawan Andi , Suardita Ketut , Zubaidah Nanik
Published: July 2020

Back Ground: Adherence of  Stretococcus mutans biofilm (S. mutans) to the surface of dental restorative materials is considered an important step in the development of secondary caries and periodontal disease. There are two type of nanofil composite: universal restorative and flowable restorative. That have different characteristic to induce S.mutans biofilm adherent in it surface.  Purpose: The aim of this study was to compare the adherence of S. mutans biofilm to two types of nanofil restorative materials, flowable restorative and universal restorative.. Materials and Methode: 32 disc-shaped specimens (∅ = 5.0 mm / thickness = 2.0 mm) of two types  composite were divided  to 4 groups (n = 8): group 1; universal restorative were immersed in pH cycling solution for 14 days ,group 2; Universal restorative  were  immersed in water for 14 day,group 3: flowable restorative were immersed in pH cycling solution,group 4: flowable restorative were immersed  for 14 days in water .  in day 15 th,  All speciments(n=32) were immersed for 24 hours in artificial saliva.. Streptococcus mutans cells were brought in contact with and grown on the speciments for 48 hours in BHI-B. Bacterial suspension was deposited onto each material and the adhesion of biofilm was evaluated trough optic density (OD) . Optic density   biofilm of S.mutans analyzed using Elissa reader’ Spectrophotometry. Statistical analysis was performed by Kruskall -wallis and Tukey HSD test  (α = 0.05). Result: Adherence of  S.mutans biofilm on flowable restorative (mean OD:1,933, SD: 0,633)  were significantly higher than universal restorative materials (mean OD: 1,240,SD:0,317). (P<0,05) Conclusion: The adherence of S.mutans biofilm on the surface of composites resin nanofil flowable restorative higher than universal restorative.
Efek Antibiofilm Glass Ionomer Cements dan Resin Modified Glass Ionomer Cements Terhadap Lactobacillus acidophilus
Authors: Halim Elsandra Novita , Samadi Karlina , Kunarti Sri
Published: July 2020

Background: Risk factors for developing secondary caries are similar to those resulting in primary caries. The marginal seal of a restoration is one of the important factors predicting clinical success. The antibiofilm effect of materials used for the luting cement of oral function affects oral health. Antibiofilm properties of dental luting materials such as Glass Ionomer Cement (GIC) and Resin Modified Glass Ionomer Cement (RMGIC) may improve the restorative treatment outcome. Purpose: This experiment evaluates the antibiofilm effect of GIC and RMGIC on Lactobacillus acidophilus in vitro. Method: Lactobacillus acidophilus served as test microorganism. The quantitative microtiter plate biofilm assays were used to evaluate the antibiofilm effect of the dental luting materials on early-stage biofilm using a direct contact test (DCT) then continued by reading of Optical Density (OD) of biofilm using ELISA reader at a wavelength of 570nm. Result: GIC and RMGIC showed a decrease of OD value from negative control in all groups. The materials’ elute had effect on both bacterial growth with GIC higher then RMGIC to inhibit Lactobacillus acidophilus biofilm formation. Conclusion: The antibiofilm effect of GIC more effective than RMGIC to inhibit Lactobacillus acidophilus biofilm formation.
EKSPRESI Interleukin-1 (IL-1) PADA PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis
Authors: Suryandari Yuliana Dwiwahyu , Suardita Ketut , Mudjiono M. , Yuanita Tamara
Published: July 2020

Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-1. IL-1 is the proinflammation cytokine that is the key of host response bacteria invation and tissue damage. Also IL-1 could cause some indirectly tissue damage through the activation of MMPS. MMPs to stop the collagen formation. Purpose. The aim of this study is to know about the expression of IL- 1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of IL-1 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of IL-1 is increasing during the periapical tissue damage that induced by E.faecalis.
EKSPRESI Tumor Necrosis Factor alpha (TNF-α) DAN Transforming growth factors beta (TGF-β) PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI Enterococcus faecalis PADA TIKUS WISTAR
Authors: Fritzgerald Richard , Lunardhi Cecilia , Effendy Ruslan , Yuanita Tamara
Published: July 2020

Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation then lead to bone destruction. LTA stimulates immunology reaction that produce Tumor Necrosis Factor alpha (TNF-α) and Transforming growth factors beta (TGF-ß). TNF-α is a main mediator and also have an important role in inflamation response otherwise TGF-ß is working as a multifunction  regulator of cell growth and differentiation during reforming and remodelling.  Purpose. The aim of this study is to know about the expression of TNF-α and TGF-ß during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 30 male rats were randomly divided into 3 main groups, Group A (control negative) : normal tooth. Group B (control positive) : every tooth was induced only by sterile BHI-b. Group C (treated group) : every tooth  was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU). The animals were sacrificed 21 days later and prepared for histological examination of tissue damage, then we did the immunohistochemistry  followed by calculation on the light microscope. Result. The analysis revealed that the expression of TNF-α at treated group are higher than negative control and positive control but the expression of  TGF-ß at treated group are higher than the negative control group but lower than positive control. Conclusion. From this study we know that the expression of TNF-α and TGF-ß are changing during the periapical tissue damage that induced by E.faecalis.
EKSPRESI MATRIKS METALLOPROTEINASE-8 DAN INTERLEUKIN-8 PADA KERUSAKAN JARINGAN PERIAPIKAL AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS
Authors: Yuanita Tamara , Radito Tantri Wismayaning , Wahjuningrum Dian Agustin , Roulianto R.
Published: July 2020

Background. The main etiology of endodontic treatment failure  is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-8 to enhance the inflamation reaction and MMP-8 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of MMP-8 and IL-8 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry  followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-8 and IL-8 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of MMP-8 and IL-8 are increasing during the periapical tissue damage that induced by E.faecalis.
Perbedaan Daya Antibakteri Antara Kombinasi Calcium Hydroxide-Based Sealer-Amoxicillin Dan Resin-Based Sealer-Amoxicillin Terhadap Bakteri Enterococcus Faecalis
Authors: Bena Asarizka , Subiwahjudi Agus , Setyabudi Setyabudi
Published: July 2020

Background. About 24-77% of root canal treatment failure cases are caused by infection of Enterococcus faecalis due to the resistance factor and virulence of these bacteria. Calcium hydroxide-based sealer and resin-based sealer are two types of sealer often used for root canal obturation due to their antibacterial properties. But the antibacterial properties owned by calcium hydroxide-based sealer has no effect on Enterococcus faecalis. Therefore, the adjunct of local antimicrobial such as amoxicillin is needed to increase antibacterial activities of root canal obturation materials (sealers) especially for calcium hydroxide-based sealer. Purpose. The aim of this study was to compare the effectiveness of antibacterial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin against Enterococcus faecalis. Methods. This study is an experimental laboratory with post test only control group design using Enterococcus faecalis ATCC 29212. Agar diffusion test was used to check the antimicrobial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin. by measuring the inhibition zone diameter of each treatment. Results. Resin-based sealer-amoxicillin’s diameter of zone inhibition is 37,3 mm and calcium hydroxide-based sealer-amoxicillin’s diameter is 18,7 mm. Conclusion. Antibacterial activity of resin-based sealer-amoxicillin is significantly greater than calcium hydroxide-based sealer-amoxicillin.
Perbedaan Daya AntibakteriEkstrak Kulit Kokoa (Theobroma cacao)dan NaOCl 2,5% terhadapPorphyromonas gingivalis
Authors: Yuanita Tamara , Jannah Rifatul , Pasetyo Edhie Arif , Setyabudi Setyabudi
Published: July 2020

Background: Since pulp infection plays an important role in the development of periradicular lesions, endodontic treatment should be directed to eliminate bacterial and theirproducts. However, currently 20% of cases of apical periodontitis are not resolved after root canal treatment and therefore required for new root canal disinfection. The most commonly used irrigation material today is NaOCl 2.5%. However, NaOCl has negative effects, including being toxic when the material is injected into the periradicular tissue causing extensive pain, bleeding and swelling. Until now, many drugs come from plants that are still produced from plant extracts. One of the plants that can be utilized is cocoa (Theobroma cacao). Cocoa contains active compounds, such as saponins, tannins, alkaloids, flanonoids, aromatic terpenoids, theobromins and other metabolites. Cocoa husk has been studied to have an antibacterial effect on Porphyromonas gingivalis which is the main bacterial cause of apical periodontal. However, the difference in antibacterial activity between cocoa husk extract and NaOCl 2.5%  againstPorphyromonas gingivalis has not been studied. Porpuse:The aim of this study is to compare antibacterial activity of cocoa husk extract and NaOCl 2.5% againstPorphyromonas gingivalis.Method: This research was a laboratory experimental study. Porphyromonas gingivalis were swabbed to nutrient agar medium. Consequently, cocoa husk extract 25% and NaOCl 2.5% were placed in wells of 5mm diameter and nutrient agar medium. The diameter of the zone of inhibition around the test materials was measured after 24 hours.Result:Cocoa husk extract has lower mean inhibitory zone diameter (14.22) than NaOCl 2.5% (16.06). Conclusion:Cocoa husk extract has lower antibacterial activity against Porphyromonas gingivalis compared to NaOCl 2.5%.
Hubungan Pengetahuan Kesehatan Gigi dan Mulut Pasien Terhadap Kepatuhan Menjalani Perawatan Berulang
Authors: Maulidah Isnia , Roelianto M , Sampoerno Galih
Published: July 2020

Background: Dental caries is one of oral and dental disease that has high prevalence in Indonesia. The fact showed that there are many dental caries were in advanced condition, resulting in root canal treatment needed. The failure of root canal treatment usually caused by patient non compliance in multivisit treatment. It was probably because of the lack of public knowledge about oral and dental health. Purpose: This study was to investigate the correlation between oral and dental health knowledge with patient compliance in multivisit treatment. Methods: An analytic survey using cross sectional design was carry out on 35 patients in UPF Konservasi Gigi RSGMP FKG UNAIR around September-November 2011. The data gathering was done using both questionnaire and focused interview. The test of Spearman correlation was used to measure the correlation between the oral and dental health knowledge to patient compliance in multivisit treatment. Result: This study showed that there was no significant correlation between oral and dental health knowledge to patient compliance (p=0,837). But there was significant correlation between patient compliance with patient motivation  (p=0,006), needs (p=0,020), perception (p=0,018) and dental treatment cost (p=0,034) with patien compliance in multivisist dental treatment. Conclusion: There was no significant correlation between oral and dental health knowledge to patient compliance. There are other factors that give significance contribution to the increasement of complianced beside knowledge, such as motivation, needs, patient’s perception in continousy treatment and and cost.
TENSILE STRENGTH OF BONDING MATERIAL TO DENTINE USING WATER-WET AND ETHANOL-WET TECHNIQUES
Authors: Nafilatus Ainin , Mudjiono M. , Saraswati Widya
Published: July 2020

Background: Composite resins have hydrophobic properties so it cannot attach to dentine. Moist dentin is a normal condition caused by the presence of fluid in dentinal tubules. Therefore bonding material is required to bond dentine with composite. Water-wet technique is a technique in which dentine surface is rinsed after etching by using water and allowing it to be moist and prevent the collapse of dentine collagen fibrils. This technique is commonly used today, but excess water disturbs the effectiveness of dentine and bonding material attachment. Ethanol-wet technique is a technique in which ethanol is used to replace water before bonding to reduce excess water in dentine surface. This technique will initiate monomer penetration into interfibrillar space of dentine. It will affect the result of tensile strength test. Purpose: The purpose of this study was to compare tensile strength of bonding material to dentine using water-wet and ethanol-wet techniques. Method: 12 samples of bovine incisors teeth were randomly divided into 2 group. Each group consists of six samples. Group 1 was treated with water-wet technique and group 2 was treated with ethanol-wet technique. Autograph was used as a tensile strength test instrument. Result : The average of tensile strength using water-wet technique was 2,16067 MPa and ethanol-wet technique was 4,21900 MPa. Conclusion: Ethanol-wet technique have higher value of tensile strength than water-wet technique.
The effect of glycerin on the surface hardness and roughness of nanofill composite
Authors: Zakiyah Diana , Effendy Ruslan , Prasetyo Edhie Arif
Published: July 2020

Background: Present research studied the surface hardness and roughness dependence on polymerization. Polymerization of composites occurs through chain reaction that is induced by free radicals. Oxygen in the air decreases the excitability of the photo initiator, causing polymerization interference. Oxygen inhibition layer (OIL) is formed on the surface. OIL can be reduced by curing the composite through by application of glycerin to the surface. Purpose: To determine the effect of glycerin on the surface hardness and roughness of nanofill composite. Material and Methods: 64 specimens of composite (Z350 XT, 3M) were prepared using a disc-shaped acrylic. The groups were divided into group A surface hardness (N=32) and group B surface roughness (N=32). Group A1, the specimen was coated with glycerin and light cured for 20 s and group A2, the specimen was exposed to air and light cured for 20 s. Group B1 was coated with glycerin and light cured for 20 s and Group B2 was exposed to air and light cured for 20 s. The specimens were stored in distilled water for 24 h at 370. Measuring with Vickers and surface roughness tester and. Data were statistically analyzed using Mean-whitney U Test. Results: There were statiscally significant difference between the surface hardness and roughness of nanofill composite coated with glycerin and without glycerin (p<0,05) Conclusion: The surface hardness of nanofill composite resin coated with glycerin is higher than composite without glycerin and the surface roughness of nanofill composite resin coated with glycerin is lower than composite without glycerin
Perbedaan ketebalan odontoblast-like cells setelah aplikasi CAPE dan Kalsium Hidroksida
Authors: Prawitasari Putri Galuh , Samadi Karlina , Subiyanto Ari
Published: July 2020

Background : CAPE is the main component of propolis, it has several biology and fpharmocological advantages as antioxidant, antiinflammation, anticancer and as an immunomodulator. There were the research before of CAPE is capable to stimulate the production of the TGF-β 1 and collagen sintesis by the pulp tissue with many superiority compare to Ca(OH)2, recently Ca(OH)2 is  the gold standard for pulp capping treatment procedure. Objective : To determine and compare the effectiveness of CAPE and Ca(OH)2 to the thickness of odontoblast-like cells in rat pulp tissue. Methods : Maxillary first molar tooth of wistar mice was class 1 preparated until the pulp opened, then Ca(OH)2 was aplicated for 14 and 28 days, CAPE for 14 and 28 days. After application of Ca(OH)2 and propolis extract, the tooth was filled with RMGIC. Teeth were extracted on defined day and processed for histological evaluation. Result : There is a significant difference in the thickness of odontoblast-like cells after application of CAPE for 14 days with Ca(OH)2 for 14 days and CAPE for 28 days with Ca(OH)2 for 28 days. Conclusion : odontoblast-like cells after aplication of CAPE extract material is thicker than Ca(OH)2.
PERBEDAAN KEKUATAN IKATAN KIMIA ANTARA BAHAN BONDING HEMA DENGAN PELARUT ASETON DENGAN BAHAN BONDING HEMA DENGAN PELARUT ETANOL PADA KOLAGEN
Authors: Soetojo Adioro , Sumarto Ivan Salomo , Mudjiono Moch. , Lunardhi Cecilia G.J.
Published: July 2020

Latar belakang: Resin komposit berikatan dengan struktur gigi melalui bahan adesif, yang dikenal dengan sebutan bahan bonding. Bahan bonding biasanya mengandung monomer fungsional yang spesifik pada umumnya adalah ester yang berasal dari reaksi alkohol bivalen dengan asam metakrilat dan phosphor atau derivate asam karboksilat.dalam studi terbaru terdapat ikatan bahan bonding secara kimia. Tujuan: Untuk menganalisis perbedaan kekuatan ikatan kimia antara bahan resin bonding berbahan dasar HEMA berpelarut etanol dan HEMA berpelarut aseton dengan kolagen dentin. Metode: Terdapat 3 kelompok pada penelitian ini. Kelompok pertama HEMA dan kolagen dicampur dengan KBr, kelompok ini sebagai kontrol positif. Kelompok kedua HEMA, etanol, dan kolagen dicampur KBr. Kelompok ketiga HEMA, aseton, dan kolagen dicampur KBr. Ketiga kelompok kemudian dijadikan pellet bening dan dianalisa dengan FTIR,untuk kemudian dihitung nilai puncak pita serapan karbonil dari masing-masing kelompok penelitian. Data dianalisa menggunakan Kruskall Wallis dan Tukey HSD test (p<0.05). Hasil: Nilai Peak (P) pita serapan karbonil kelompok I: 71.36, kelompok II : 46.76, kelompok III : 27.2. Semakin kecil nilai Peak (P) pita serapan karbonil maka diamsusikan semakin besar kekuatan ikatan secara kimia. Kesimpulan: Kekuatan ikatan kimia pada bahan bonding HEMA berpelarut aseton lebih besar dibandingkan dengan berpelarut etanol pada kolagen dentin.
Daya Antibakteri Ekstrak Batang Pisang Ambon (Musa paradisiaca var. sapientum) terhadap Pertumbuhan Enterococcus faecalis
Authors: Hafizha H. , Suardita Ketut , Pribadi Nirawati
Published: July 2020

Background: Enterococcus faecalis is one of the most common bacteria in root canal after endodontic treatment. This species is found in about 77% of the cases that resistant to treatment. It is necessary to develop an alternative for intracanal dressing, one of the potential substance is natural ingredients. The stem of ambonese banana (Musa paradisiaca var. sapientum) extract contains active substances such as tannin, flavonoid, alkaloid, saponin, and antraquinone, which have been known for their antibacterial potency. Purpose: The  aim of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ambonese banana stem (Musa paradisiaca var. sapientum) extract against Enterococcus faecalis. Method: This research was a laboratory experimental study. Ambonese banana stem extract was made by maceration method with ethanol 96% and certain dilution was performed to obtain various concentration. Value of MIC and MBC of ambonese banana stem extract against Enterococcus faecalis were known by counting the growth of bacteria colonies on blood agar media in CFU/ml. Result: The percentages of bacteria colonies at concentration 85%; 82,5%; 80%; 77,5%; 75%; 72,5%; 70%; and 67,5% of ambonese banana stem extract in sequence were 0%; 0%; 0%; 0%; 0%; 0%; 7,44%; and 14,53% from the positive control. Conclusion: The stem of ambonese banana (Musa paradisiaca var. sapientum) extract had minimum inhibitory concentration (MIC) at 70% concentration and minimum bactericidal concentration (MBC) at 72,5% concentration against Enterococcus faecalis.
EFFICACY OF DIODE LASSER 405 NM WITH CHLOROPHYLLS AS PHOTOSENSITIZER ON Enterococcus faecalis
Authors: Kunarti Sri , Tjandra Amellia , Prasetyo Edhie Arif
Published: July 2020

Background: The presence of persistent infections in the root canals by microorganisms causes root canal failure. The most commonly found bacteria that cause persistent infection is Enterococcus faecalis. PDI / photodynamic inactivation is an in vitro approach to inactivation of microorganisms. The combination of light and photosensitivity of chlorophyll in PDI will cause photoinactivation in bacteria. Long radiation of PDT can affect the production of singlet oxygen and ROS (Reactive Oxygen Species) to kill Enterococcus faecalis bacteria. Objective: To prove the effect of 405 nm laser diode  with and without chlorophyll photosensitizer and the irradiation effect of 405 nm laser diode on the number of CFU of Enterococcus faecalis bacteria. Method: This study used the Enterococcus faecalis bacteria cultur  which was divided into 5 groups. Group I as control group, Group II irradiation 30 ', III chlorophyll + irradiation 30', IV irradiation 60 ', V chlorophyll + irradiation 60'. After incubation, the bacteria count was calculated with Quebec Colony Counter and analyzed by Shapiro-Wilk test, Levene test and Anova test. Results: There were significant differences (p <0.05) between the number of colonies of Enterococcus faecalis bacteria in each treatment group. Longer duration of PDT exposure (Group II and IV) with chlorophyll showed less number of Enterococcus faecalis bacteria. Conclusion: The longer the PDT irradiation, the less number of Enterococcus faecalis bacteria. The 60-second radiation with chlorophyll showed the least amount of Enterococcus faecalis bacteria.
Perbedaan efektifitas penutupan tubulus dentin antara pasta gigi yang mengandung bioaktif glass (novamin) dan strontium chloride
Authors: Shinta Profilia , Suardita Ketut , Mudjiono Moch.
Published: July 2020

Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized by pain arising from exposed dentin in response to various stimuli. Various treatment modalities are available to treat dentinal hypersensitivity which include at-home and in-office treatment. At home treatment generally consists of a variety of dentrifices containing different constituents like strontium chloride and bioactive glass (Novamin). These agents cause occlusion of dentinal tubules thereby reducing hypersensitivity.Purpose: This study  was  to evaluate the effects of different desensitizing dentifrices on dentinal tubule occlusion by scanning electron microscopy (SEM) of strontium chloride and bioactive glass (Novamin) pastes in the treatment of dentinehypersensitivity (DH) Methods: sixteen extractedspecimens from bovine incisors teeth with randomized into 2 groups (n=8). The crowns were removed from the root and the crown  were sectioned longitudinally into two parts (in a mesiodistaldirection). The cervical toothwere resulting in two samples per tooth.Dentinal tubules were exposedand thesamples provided a 4 mm x 4 mm area of exposed dentinal tubules.Group 1 (treated with strontium chloride), Group 2 (treated with  bioactive glass (novamin))After each treatment for seven days, tubule occlusion on dentin were analyzed by scanning electron microscopy  (SEM).The data were analyzed using “mann-whitneytest” (p<0.05).Result: Groups bioactive glass (novamin) showed tubule occlusion highest when compared with groups strontium chloride.Conclusion: bioactive glass (novamin) paste showed tubule occlusion highest with strontium chloride
Adsorption Studies of Rice Husk-Based Silica/Carbon Composite
Authors: Rengga Wara Dyah Pita , Sediawan Wahyudi Budi , Imani Nadya Alfa Cahaya , Harianingsih Harianingsih , Salsabiil Kega Amalania
Published: July 2020

Rice husk as a by-product of the rice milling process is a material that can still be processed to be a product that can be used in various applications. The potential for the commercialization ofrice husks is still hampered by its low economic value. By using the heating method using an inert gas, rice husk can be converted into a composite which contains mostly silica and carbon, a wellknown component with excellent adsorbent properties. This aimed to study the preparation of rice husk-baased silica/carbon composite and its adsorption behavior. In this research, the utilization of rice husk into silica/carbon composites was done by using three different methods. Common heating under temperature of 800 oC, heating process under temperature of 800 oC using furnace equipped with inert gas, and heating process under temperature of 800 oC using furnace equipped with inert gas with addition of glucose solution were carried out in order to obtain three different kinds of composite. Analysis and characterization using were performed to observe the properties of the composites. As the result, from Fourier Transform Infra-Red and X-Ray diffraction characterizations, silica/carbon composites were successfully made as both of carbon and amorphous silica spectra appeared in the result. Furthermore, composite made under temperature of 800 oC using furnace equipped with inert gas with addition of glucose solution has the highest adsorption capacity and Langmuir’s model adsorptive behavior with R2 value of 0.935.
Development of Stages of The Implementation of The Environmental Monitoring Program
Authors: Nazila Rahimova , Vugar Abdullayev , Vasila Abbasova
Published: July 2020

The object of the research is environmental monitoring, which allows to present the current situation about the environment. The monitoring program aims to gather information for the decision-making process. This article describes environmental monitoring and highlights its main goals and objectives. This article describes the properties of the environmental monitoring object. The main components of environmental monitoring are also considered here. The database and analytical data-processing tools are main components of environmental monitoring. In addition, this article considers the structure of environmental monitoring. Next, environmental monitoring functions were considered. This article also provided a classification of monitoring systems. One problem is the development of the most appropriate algorithm for implementing environmental monitoring. The article is devoted to the development of software modules of environmental information systems that perform geographic and environmental data processing. There are software modules designed for centralized operation of data (data entry, editing, deleting, etc.), the conceptual types of GIS, the goals and functions of environmental monitoring, and these are taken into account in the existing system. The monitoring system should also have a good search tool, which is also described in this article. The search tool is described using SQL and Delphi. The research used decision-making methods. This article describes the stages of development of an environmental monitoring program. The research identified the inputs and outputs of the environmental monitoring programme, as well as its objects and the relationship between them. The results obtained in the future will be useful for improving the decision-making of authorized persons.
Анализ геномного разнообразия SARS-Cov-2 и эпидемиологических признаков адаптации возбудителя COVID-19 к человеческой популяции (Сообщение 1).
Authors: Краснов Ярослав
Published: July 2020

Вспышка COVID-19 началась в середине декабря 2019 года в китайском городе Ухань и быстро переросла в пандемию, продолжающуюся по настоящее время. В этом исследовании мы проанализировали информацию о 7947 полных геномах вируса SARS-CoV-2, опубликованную в базе GISAID, для изучения направления эволюции вируса при адаптации к организму человека за последние шесть месяцев. Проведен филогенетический анализ и оценка изменений в вирусных геномах, которые могут быть разделены на 4 основные кластера. Ключевое направление эволюции генома вируса SARS-CoV-2 сопряжено с появлением единичной мутации в гене S (D614G). Установлено, что распространение штаммов с G мутацией ассоциировано не только с ростом заболеваемости, но и снижением летальности в мире. Наблюдаемое увеличение потенциала распространения на фоне признаков снижения вирулентности, вероятно, является основной формой адаптации нового коронавируса к человеческой популяции и, по видимому, будет продолжаться в дальнейшем в виде интеграции SARS-CoV-2 в структуру сезонных возбудителей ОРВИ.
Contents
Authors: Wilson Robert
Published: July 2020

Contents
Editorial
Authors: Wilson Robert
Published: July 2020

Editorial
Calcifying Fibrous Tumor of the Jejunum in a 27-year-old Primigravid
Authors: Masalunga Marvin , Carnate Jr. Jose
Published: July 2020

The most common mesenchymal tumors of the gastrointestinal tract are gastrointestinal stromal tumors (GIST) and smooth muscle neoplasms; however, other soft tissue tumors may also present in the intestines and cause diagnostic dilemmas. We report the case of a 27-year old primigravid, with no known complications, who underwent cesarean section for cephalopelvic disproportion. Intraoperatively, a well-demarcated, solid mass measuring 1.5 x 1.0 x 0.7 cm was noted at the jejunum. The patient underwent segmental resection of the mass. Microscopic examination of the mass reveals a non-encapsulated, solid mass composed of bland spindle cells and dense, hyalinized collagen in whorls and bundles. Dystrophic calcifications and a lymphoplasmacytic inflammatory infiltrate are seen within the collagen bundles. Immunohistochemical staining with desmin, CD117, and DOG-1 was done, which are all negative. The case was signed out as calcifying fibrous tumor (CFT). Inclusion of CFTs in the differential diagnoses for mesenchymal tumors of the gastrointestinal tract is important, as these neoplasms are benign and have an excellent prognosis.
Establishing Synchronous Primary Tumors of the Urothelium and Prostate by Immunohistomorphology
Authors: Ong David Jerome , Alcazaren Elizabeth Ann , Carnate Jr. Jose
Published: July 2020

Synchronous primary tumors of the urothelium and prostate are a diagnostic challenge amongpathologists. Differentiating carcinomas of urothelial and prostatic origin requires careful assessment of histomorphology coupled with ancillary studies such as immunohistochemistry stains (IHC) to support the diagnosis. We report two cases of adult patients who underwent transurethral resection of the prostate (TURP), with two distinct morphologies noted on routine H&E sections. After a panel of immunohistochemical stains (HMWCK, CK5/6, CK7, CK20, GATA-3, p63, NKX3.1, and PSA), both cases were signed out as papillary urothelial carcinoma and prostatic acinar adenocarcinoma. Correlation of histomorphology with an IHC panel consisting of cytokeratins (CK5/6, CK7, CK20), a urothelial marker (GATA-3), and at least two prostatic markers (PSA, NKX3.1) is recommended in such cases.
Regulación o desregulación: una reflexión desde el design thinking
Authors: Méndez Reátegui Rubén , Álvarez Meythaler Amparo
Published: July 2020

El design thinking, como proceso y metodología, se centra en el usuario. Es una propuesta de proceso libre pero sistemático de gestión de la creatividad para la innovación en cinco etapas. Una vez aplicado, transforma la «práctica tradicional» de la generación de ideas hacia un pensamiento basado en la comprensión profunda de las necesidades del usuario. Bajo estos lineamientos, el artículo propondrá al design thinking como una herramienta metodológica aplicable en la práctica del derecho, y esbozará brevemente su ejemplificación en ámbitos como la práctica regulatoria o desregulatoria.
La metáfora y su aporte para el estudio de la melancolía como confrontación de la cultura política en Ni pan ni circo (2005)
Authors: Pequeño Saco Tamara Paloma
Published: July 2020

Este trabajo reflexiona, en primer término, desde la perspectiva de la cultura, sobre el concepto de melancolía, como consecuencia del fracaso de la utopía de izquierda nacida de la ética de los sectores subalternos. Luego analiza la posibilidad de examinar los discursos literarios no hegemónicos para obtener información sobre la subjetividad con que sus creadores perciben el contexto a partir de la memoria afectada por la derrota política. Para este propósito analizamos el papel que cumple la metáfora en los poemas de Alejandro Romualdo de su libro Ni pan ni circo (2005). Empleamos como marco teórico las propuestas de Roger Bartra (2001) en su Cultura y melancolía. Las enfermedad del alma en la España del Siglo de Oro, además de los aportes de Raymond Williams (2000), Robert Burton (2015), Enzo Traverso (2019) y Walter Benjamin (2008).
Barriers, enablers and strategies for the treatment and control of hypertension in Nepal: a mixed-methods systematic review
Authors: Dhungana Raja Ram , Pedisic Zeljko , Pandey Achyut Raj , Shrestha Nipun , de Courten Maximilian
Published: July 2020

Background: The challenge of achieving effective treatment and control of blood pressure is linked to various barriers to hypertension care at different layers of health system (HS). Evidence is emerging globally on the effectiveness of multi-pronged hypertension control strategies. However, no systematic review of strategies of and factors associated with hypertension treatment and control in Nepal is available. Understanding country-specific factors influencing hypertension care is critical to address the gaps in the management of hypertension. This study aimed to systematically review published literature and synthesise the findings on barriers, enablers and strategies for hypertension treatment and control in Nepal. Methods: Six databases namely Embase, PubMed, Web of Science, CINAHL, ProQuest and WorldCat, Nepali journals and Nepal government websites were systematically searched for qualitative, quantitative and mixed-methods studies investigating the factors or strategies in relation to hypertension treatment and control in Nepal. The methodological quality of selected articles was assessed using Mixed Methods Appraisal Tool. Themes on barriers and enablers were generated and framed under “health system” , “provider” and “patient” domains, according to the framework synthesis approach. Findings on hypertension strategies were narratively synthesised. Results: We identified 14 published studies; one with mixed, three with qualitative and 10 with quantitative methods. Eight were related to barriers and enablers and six of hypertension strategies. The identified barriers associated with the HS were: lack of affordable services and lack of resources. The barriers at the provider’s level were: communication gaps and long waiting hours for appointments. Poor help-seeking behaviour, non-adherence to medication, perceived side-effects of drugs, financial hardship and lack of family support were barriers identified at patient level. The following enablers were identified: positive illness perception, free essential healthcare services and family support. Strategies implemented across the HS, provider and patient were: establishing digital health records at health centres, health worker’s capacity development, health education and yoga practice. Conclusion: There is a range of barriers for hypertension treatment and control in Nepal pertaining to the HS, providers, and patients. Comprehensive interventions are needed at all three levels to further improve management and control of hypertension in Nepal.Registration: The protocol is registered in PROSPERO (registration number: CRD42020145823)
Effect of Maternal Sleep, Physical Activity and Screen Time during Pregnancy on the Risk of Childhood Respiratory Allergies: a Sex-specific Study
Authors: Chen Yiting , Lyu Jiajun , Xia Yuanqing , Zhu Jianzhen , Tong Shilu , Yin Yong , Qu Jiajie , Li Shenghui
Published: July 2020

Background: Early life exposure in the uterus had long-term effect on children's health. As the prevalence of allergies is increasing with a remarkable gender difference, very few studies have traced back to their early origins. We sought to investigate if maternal behavioral exposure, herein sleep, physical activity and screen time during pregnancy, is associated childhood respiratory allergies. The sex difference would be examined. Methods: 6236 mother-child pairs from Shanghai Children Allergy Study (SCAS) were enrolled, The International Study of Asthma and Allergies in Childhood questionnaire was adopted to evaluate respiratory allergic diseases. Results: 14.6%, 16.2% and 21.0% of children had asthma, wheeze, and allergic rhinitis, respectively. Maternal short sleep duration, lack of physical activity and too much screen exposure during pregnancy could increase the risk of childhood respiratory allergies. Moreover, a dose-response trend was clearly shown. However, the significance was found only in males, any two of the three combined could increase the risk (OR,1.921; 95%CI,1.217-3.033), and the coexistence of all three further amplified the risk (OR,2.412; 95%CI,1.489-3.906). The findings can be verified in allergen test subgroup and single type of respiratory allergies in most cases. And gender difference was identified, all above associations were mainly observed in males. Conclusions: Maternal unhealthy behaviors during pregnancy could increase the risk of childhood respiratory allergies with a dose-response pattern. Males were more susceptible to the association. The identification of modifiable maternal risk behaviors lies in the emphasis of intervention in early life to face up increasing childhood allergies.