bluuebunny/crossref_metadata_2025 huggingface dataset:
JCPyV-Induced MAPK Signaling Activates Transcription Factors during Infection
Authors: DuShane Jeanne K. , Mayberry Colleen L. , Wilczek Michael P. , Nichols Sarah L. , Maginnis Melissa S.
Published: September 2019

JC polyomavirus (JCPyV), a ubiquitous human pathogen, is the etiological agent of the fatal neurodegenerative disease progressive multifocal leukoencephalopathy (PML). Like most viruses, JCPyV infection requires the activation of host-cell signaling pathways in order to promote viral replication processes. Previous works have established the necessity of the extracellular signal-regulated kinase (ERK), the terminal core kinase of the mitogen-activated protein kinase (MAPK) cascade (MAPK-ERK) for facilitating transcription of the JCPyV genome. However, the underlying mechanisms by which the MAPK-ERK pathway becomes activated and induces viral transcription are poorly understood. Treatment of cells with siRNAs specific for Raf and MAP kinase kinase (MEK) targets proteins in the MAPK-ERK cascade, significantly reducing JCPyV infection. MEK, the dual-specificity kinase responsible for the phosphorylation of ERK, is phosphorylated at times congruent with early events in the virus infectious cycle. Moreover, a MAPK-specific signaling array revealed that transcription factors downstream of the MAPK cascade, including cMyc and SMAD4, are upregulated within infected cells. Confocal microscopy analysis demonstrated that cMyc and SMAD4 shuttle to the nucleus during infection, and nuclear localization is reduced when ERK is inhibited. These findings suggest that JCPyV induction of the MAPK-ERK pathway is mediated by Raf and MEK and leads to the activation of downstream transcription factors during infection. This study further defines the role of the MAPK cascade during JCPyV infection and the downstream signaling consequences, illuminating kinases as potential therapeutic targets for viral infection.
Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen
Authors: Hwang Sungmin , Chavarria Nikita , Hackley Rylee , Schmid Amy , Maupin-Furlow Julie
Published: September 2019

Haloferax volcanii, a well-developed model archaeon for genomic, transcriptomic, and proteomic analyses, can grow on a defined medium of abundant and intermediate levels of fixed nitrogen. Here we report a global profiling of gene expression of H. volcanii grown on ammonium as an abundant source of fixed nitrogen compared to l-alanine, the latter of which exemplifies an intermediate source of nitrogen that can be obtained from dead cells in natural habitats. By comparing the two growth conditions, 30 genes were found to be differentially expressed, including 16 genes associated with amino acid metabolism and transport. The gene expression profiles contributed to mapping ammonium and l-alanine usage with respect to transporters and metabolic pathways. In addition, conserved DNA motifs were identified in the putative promoter regions and transcription factors were found to be in synteny with the differentially expressed genes, leading us to propose regulons of transcriptionally co-regulated operons. This study provides insight to how H. volcanii responds to and utilizes intermediate vs. abundant sources of fixed nitrogen for growth, with implications for conserved functions in related halophilic archaea.
The Organogermanium Compound THGP Suppresses Melanin Synthesis via Complex Formation with L-DOPA on Mushroom Tyrosinase and in B16 4A5 Melanoma Cells
Authors: Azumi Junya , Takeda Tomoya , Shimada Yasuhiro , Aso Hisashi , Nakamura Takashi
Published: September 2019

The organogermanium compound 3-(trihydroxygermyl)propanoic acid (THGP) has various biological activities. We previously reported that THGP forms a complex with cis-diol structures. L-3,4-Dihydroxyphenylalanine (L-DOPA), a precursor of melanin, contains a cis-diol structure in its catechol skeleton, and excessive melanin production causes skin darkening and staining. Thus, the cosmetic field is investigating substances that suppress melanin production. In this study, we investigated whether THGP inhibits melanin synthesis via the formation of a complex with L-DOPA using mushroom tyrosinase and B16 4A5 melanoma cells. The ability of THGP to interact with L-DOPA was analyzed by 1H-NMR, and the influence of THGP and/or kojic acid on melanin synthesis was investigated. We also examined the effect of THGP on cytotoxicity, tyrosinase activity, and gene expression and found that THGP interacted with L-DOPA, a precursor of melanin with a cis-diol structure. The results also showed that THGP inhibited melanin synthesis, exerted a synergistic effect with kojic acid, and did not affect tyrosinase activity or gene expression. These results suggest that THGP is a useful substrate that functions as an inhibitor of melanogenesis and that its effect is enhanced by combination with kojic acid.
Rhodococcus as a Versatile Biocatalyst in Organic Synthesis
Authors: Busch Hanna , Hagedoorn Peter-Leon , Hanefeld Ulf
Published: September 2019

The application of purified enzymes as well as whole-cell biocatalysts in synthetic organic chemistry is becoming more and more popular, and both academia and industry are keen on finding and developing novel enzymes capable of performing otherwise impossible or challenging reactions. The diverse genus Rhodococcus offers a multitude of promising enzymes, which therefore makes it one of the key bacterial hosts in many areas of research. This review focused on the broad utilization potential of the genus Rhodococcus in organic chemistry, thereby particularly highlighting the specific enzyme classes exploited and the reactions they catalyze. Additionally, close attention was paid to the substrate scope that each enzyme class covers. Overall, a comprehensive overview of the applicability of the genus Rhodococcus is provided, which puts this versatile microorganism in the spotlight of further research.
Polyamine Catabolism in Acute Kidney Injury
Authors: Zahedi Kamyar , Barone Sharon , Soleimani Manoocher
Published: September 2019

Acute kidney injury (AKI) refers to an abrupt decrease in kidney function. It affects approximately 7% of all hospitalized patients and almost 35% of intensive care patients. Mortality from acute kidney injury remains high, particularly in critically ill patients, where it can be more than 50%. The primary causes of AKI include ischemia/reperfusion (I/R), sepsis, or nephrotoxicity; however, AKI patients may present with a complicated etiology where many of the aforementioned conditions co-exist. Multiple bio-markers associated with renal damage, as well as metabolic and signal transduction pathways that are involved in the mediation of renal dysfunction have been identified as a result of the examination of models, patient samples, and clinical data of AKI of disparate etiologies. These discoveries have enhanced our ability to diagnose AKIs and to begin to elucidate the mechanisms involved in their pathogenesis. Studies in our laboratory revealed that the expression and activity of spermine/spermidine N1-acetyltransferase (SAT1), the rate-limiting enzyme in polyamine back conversion, were enhanced in kidneys of rats after I/R injury. Additional studies revealed that the expression of spermine oxidase (SMOX), another critical enzyme in polyamine catabolism, is also elevated in the kidney and other organs subjected to I/R, septic, toxic, and traumatic injuries. The maladaptive role of polyamine catabolism in the mediation of AKI and other injuries has been clearly demonstrated. This review will examine the biochemical and mechanistic basis of tissue damage brought about by enhanced polyamine degradation and discuss the potential of therapeutic interventions that target polyamine catabolic enzymes or their byproducts for the treatment of AKI.
Lysine Propionylation is a Widespread Post-Translational Modification Involved in Regulation of Photosynthesis and Metabolism in Cyanobacteria
Authors: Yang Mingkun , Huang Hui , Ge Feng
Published: September 2019

Lysine propionylation is a reversible and widely distributed post-translational modification that is known to play a regulatory role in both eukaryotes and prokaryotes. However, the extent and function of lysine propionylation in photosynthetic organisms remains unclear. Cyanobacteria are the most ancient group of Gram-negative bacteria capable of oxygenic photosynthesis, and are of great importance to global carbon and nitrogen cycles. Here, we carried out a systematic study of lysine propionylaiton in cyanobacteria where we used Synechocystis sp. PCC 6803 (Synechocystis) as a model. Combining high-affinity anti-propionyllysine pan antibodies with high-accuracy mass spectrometry (MS) analysis, we identified 111 unique lysine propionylation sites on 69 proteins in Synechocystis. Further bioinformatic analysis showed that a large fraction of the propionylated proteins were involved in photosynthesis and metabolism. The functional significance of lysine propionylation on the enzymatic activity of fructose-1,6-bisphosphatase (FbpI) was studied by site-directed mutagenesis and biochemical studies. Further functional studies revealed that the propionylation level of subunit II of photosystem I (PsaD) was obviously increased after high light (HL) treatment, suggesting that propionylation may be involved in high light adaption in Synechocystis. Thus, our findings provide novel insights into the range of functions regulated by propionylation and reveal that reversible propionylation is a functional modification with the potential to regulate photosynthesis and carbon metabolism in Synechocystis, as well as in other photosynthetic organisms.
Correction: Li, et al. Conserved miR396b-GRF Regulation Is Involved in Abiotic Stress Responses in Pitaya (Hylocereus polyrhizus). Int. J. Mol. Sci. 2019, 20, 2501
Authors: Li A-Li , Wen Zhuang , Yang Kun , Wen Xiao-Peng
Published: September 2019

The authors wish to make the following corrections to this paper [...]
Extracellular miRNAs as Biomarkers of Head and Neck Cancer Progression and Metastasis
Authors: Nowicka Zuzanna , Stawiski Konrad , Tomasik Bartłomiej , Fendler Wojciech
Published: September 2019

Head and neck squamous cell carcinomas (HNSCCs) contribute to over 300,000 deaths every year worldwide. Although the survival rates have improved in some groups of patients, mostly due to new treatment options and the increasing percentage of human papillomavirus (HPV)-related cancers, local recurrences and second primary tumors remain a great challenge for the clinicians. Presently, there is no biomarker for patient surveillance that could help identify patients with HNSCC that are more likely to experience a relapse or early progression, potentially requiring closer follow-up or salvage treatment. MicoRNAs (miRNAs) are non-coding RNA molecules that posttranscriptionally modulate gene expression. They are highly stable and their level can be measured in biofluids including serum, plasma, and saliva, enabling quick results and allowing for repeated analysis during and after the completion of therapy. This has cemented the role of miRNAs as biomarkers with a huge potential in oncology. Since altered miRNA expression was described in HNSCC and many miRNAs play a role in radio- and chemotherapy resistance, cancer progression, and metastasis, they can be utilized as biomarkers of these phenomena. This review outlines recent discoveries in the field of extracellular miRNA-based biomarkers of HNSCC progression and metastasis, with a special focus on HPV-related cancers and radioresistance.
Fabrication of Surfactant-Dispersed HiPco Single-Walled Carbon Nanotube-Based Alginate Hydrogel Composites as Cellular Products
Authors: Alvarez-Primo Fabian , Anil Kumar Shweta , Manciu Felicia S. , Joddar Binata
Published: September 2019

In this study, we designed, synthesized, and characterized ultrahigh purity single-walled carbon nanotube (SWCNT)-alginate hydrogel composites. Among the parameters of importance in the formation of an alginate-based hydrogel composite with single-walled carbon nanotubes, are their varying degrees of purity, their particulate agglomeration and their dose-dependent correlation to cell viability, all of which have an impact on the resultant composite’s efficiency and effectiveness towards cell-therapy. To promote their homogenous dispersion by preventing agglomeration of the SWCNT, three different surfactants-sodium dodecyl sulfate (SDS-anionic), cetyltrimethylammonium bromide (CTAB-cationic), and Pluronic F108 (nonionic)-were utilized. After mixing of the SWCNT-surfactant with alginate, the mixtures were cross-linked using divalent calcium ions and characterized using Raman spectroscopy. Rheometric analysis showed an increase in complex viscosity, loss, and storage moduli of the SWCNT composite gels in comparison with pure alginate gels. Scanning electron microscopy revealed the presence of a well-distributed porous structure, and all SWCNT-gel composites depicted enhanced electrical conductivity with respect to alginate gels. To characterize their biocompatibility, cardiomyocytes were cultured atop these SWCNT-gels. Results comprehensively implied that Pluronic F108 was most efficient in preventing agglomeration of the SWCNTs in the alginate matrix, leading to a stable scaffold formation without posing any toxicity to the cells.
Molecular Characterization of the Transcription Factors in Susceptible Poplar Infected with Virulent Melampsora larici-populina
Authors: Chen Qiaoli , Wang Jianan , Li Danlei , Wang Zhiying , Wang Feng , Zhang Ruizhi
Published: September 2019

Transcription factors (TFs) have been shown to play important roles in determining poplar susceptibility. In this study, the transcript profiles of five resistance-related TF groups at different time points were investigated to study the roles of TFs in the compatible interaction between ‘Robusta’ (Populus nigra × P. deltoides) and the virulent E4 race of Melampsora larici-populina. The susceptibility test indicated that the parasitic process of E4 could be divided into two representative time periods: the infection phase and the production phase. Bioinformatics analysis showed that in these two phases, E4 infection induced a network of TFs in ‘Robusta’. Although some TFs responded rapidly and positively, most TFs did not respond to E4, especially during the infection phase. The ethylene, jasmonic acid, and auxin pathways were downregulated, while a calcium-binding protein was upregulated. No other significantly changed phytohormone-related genes were found, which was consistent with the pathological process in the absence of an immune response, suggesting that the lack of response of most TFs during the infection phase of E4 is related to the susceptibility of ‘Robusta’.
A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration
Authors: Su Feng , Spee Christine , Araujo Eduardo , Barron Eric , Wang Mo , Ghione Caleb , Hinton David R. , Nusinowitz Steven , Kannan Ram , Reddy Srinivasa T. , Farias-Eisner Robin
Published: September 2019

Age-related macular degeneration (AMD) is a leading cause of blindness in the developed world. The retinal pigment epithelium (RPE) is a critical site of pathology in AMD. Oxidative stress plays a key role in the development of AMD. We generated a chimeric high-density lipoprotein (HDL), mimetic peptide named HM-10/10, with anti-oxidant properties and investigated its potential for the treatment of retinal disease using cell culture and animal models of RPE and photoreceptor (PR) degeneration. Treatment with HM-10/10 peptide prevented human fetal RPE cell death caused by tert-Butyl hydroperoxide (tBH)-induced oxidative stress and sodium iodate (NaIO3), which causes RPE atrophy and is a model of geographic atrophy in mice. We also show that HM-10/10 peptide ameliorated photoreceptor cell death and significantly improved retinal function in a mouse model of N-methyl-N-nitrosourea (MNU)-induced PR degeneration. Our results demonstrate that HM-10/10 protects RPE and retina from oxidant injury and can serve as a potential therapeutic agent for the treatment of retinal degeneration.
Proteasome Inhibitors Suppress ErbB Family Expression through HSP90-Mediated Lysosomal Degradation
Authors: Huynh Thanh Kieu , Ho Chien-Yi , Tsai Chi-Hua , Wang Chien-Kuo , Chen Yun-Ju , Bau Da-Tian , Tu Chih-Yen , Li Tzong-Shiun , Huang Wei-Chien
Published: September 2019

Although dual EGFR/HER2 tyrosine kinase inhibitor lapatinib has provided effective clinical benefits for HER2-positive breast cancer patients, acquired resistance to this drug remains a major concern. Thus, the development of alternative therapeutic strategies is urgently needed for patients who failed lapatinib treatment. Proteasome inhibitors have been reported to possess high anti-tumor activity to breast cancer cells. Therefore, this study aims to examine whether and how proteasome inhibitor bortezomib can overcome lapatinib resistance. Treatments with several proteasome inhibitors, including Bortezomib, MG132, and proteasome inhibitor I (PSI), as well as the viabilities of both HER2-positive breast cancer cell lines and their lapatinib-resistant clones, were inhibited. Importantly, the expressions of ErbB family were downregulated at both transcriptional and translational levels. Also, our results further indicated that proteasome inhibitors decreased ErbB family expression through lysosomal degradation pathway in a heat shock protein 90 (HSP90)-dependent manner. In this study, our data supported a potential approach to overcome the acquired resistance of HER2-overexpressing breast cancer patients to lapatinib using proteasome inhibitors.
Reliability of miRNA Analysis from Fixed and Paraffin-Embedded Tissues
Authors: Azzalini Eros , De Martino Eleonora , Fattorini Paolo , Canzonieri Vincenzo , Stanta Giorgio , Bonin Serena
Published: September 2019

In clinical practice, patients’ tissues are fixed and paraffin-embedded in order to enable histological diagnosis. Nowadays, those tissues are also used for molecular characterization. Formalin is the most used fixative worldwide, and Bouin’s solution in some worldwide institutions. Among molecular targets, micro RNAs (miRNAs), the single-stranded non-coding RNAs comprised of 18 to 24 nucleotides, have been demonstrated to be resistant to fixation and paraffin-embedding processes, with consequent possible application in clinical practice. In the present study, let-7e-5p, miR-423-3p, miR-92a-1-5p, miR-30d-5p, miR-155-5p, miR-200a-3p, and miR-429 were investigated in formalin and matched Bouin’s solution-fixed tissues of high grade serous ovarian cancers by means of real-time and droplet digital PCR (ddPCR). Micro RNAs were detectable and analyzable in both formalin- and Bouin’s-fixed specimens, but on average, higher Ct values and lower copies/µL were found in Bouin’s-fixed samples. Data from formalin-fixed samples correlated significantly for most targets with Bouin’s ones, except for let-7e-5p and miR-155-5p. This study shows that miRNAs are analyzable in both formalin- and Bouin’s-fixed specimens, with the possibility, after proper data normalization, to compare miRNA-based data from formalin-fixed samples to those of Bouin’s-fixed ones.
Evaluation of NEON Data to Model Spatio-Temporal Tick Dynamics in Florida
Authors: Klarenberg Geraldine , Wisely Samantha M.
Published: September 2019

In 2013, the National Ecological Observatory Network (NEON) started collecting 30-year multi-faceted ecological data at various spatial and temporal scales across the US including ticks. Understanding the abundance and dynamics of disease vectors under changing environmental conditions in the long-term is important to societies, but sustained long-term collection efforts are sparse. Using hard-bodied tick data collected by NEON, the vegetation and atmospheric data and a statistical state-space model, which included a detection probability component, this study estimated the abundance of tick nymphs and adult ticks across a Florida NEON location. It took into account the spatial and temporal variation, and factors affecting detection. Its purpose was to test the applicability of data collected thus far and evaluate tick abundance. The study found an increase in tick abundance at this Florida location, and was able to explain spatial and temporal variability in abundance and detection. This approach shows the potential of NEON data. The NEON data collection is unique in scale, and promises to be of great value to understand tick and disease dynamics across the US. From a public health perspective, the detection probability of vectors can be interpreted as the probability of encountering that vector, making these types of analyses useful for estimating disease risk.
The Error of Estimated GFR in Type 2 Diabetes Mellitus
Authors: Luis-Lima Sergio , Higueras Linares Tomás , Henríquez-Gómez Laura , Alonso-Pescoso Raquel , Jimenez Angeles , López-Hijazo Asunción María , Negrín-Mena Natalia , Martín Candelaria , Sánchez-Gallego Macarena , Galindo-Hernández Sara Judith , Socas Fernández del Castillo Raquel , Castilla-Marrero Manuel , Domínguez-Coello Santiago , Vilchez de León Vanesa , Valcárcel-Lopez Rafael , Insausti-Garmendia Nerea , Escamilla Beatriz , Estupiñán Sara , Delgado-Mallén Patricia , Armas-Padrón Ana-María , Marrero-Miranda Domingo , González-Rinne Ana , Miquel Rodríguez Rosa María , Cobo-Caso María Angeles , Díaz-Martín Laura , González-Rinne Federico , González-Delgado Alejandra , López-Martínez Marina , Jiménez-Sosa Alejandro , Torres Armando , Porrini Esteban
Published: September 2019

Type 2 diabetes mellitus represents 30–50% of the cases of end stage renal disease worldwide. Thus, a correct evaluation of renal function in patients with diabetes is crucial to prevent or ameliorate diabetes-associated kidney disease. The reliability of formulas to estimate renal function is still unclear, in particular, those new equations based on cystatin-C or the combination of creatinine and cystatin-C. We aimed to assess the error of the available formulas to estimate glomerular filtration rate in diabetic patients. We evaluated the error of creatinine and/or cystatin-C based formulas in reflecting real renal function over a wide range of glomerular filtration rate (from advanced chronic kidney disease to hyperfiltration). The error of estimated glomerular filtration rate by any equation was common and wide averaging 30% of real renal function, and larger in patients with measured glomerular filtration rate below 60 mL/min. This led to chronic kidney disease stages misclassification in about 30% of the individuals and failed to detect 25% of the cases with hyperfiltration. Cystatin-C based formulas did not outperform creatinine based equations, and the reliability of more modern algorithms proved to be as poor as older equations. Formulas failed in reflecting renal function in type 2 diabetes mellitus. Caution is needed with the use of these formulas in patients with diabetes, a population at high risk for kidney disease. Whenever possible, the use of a gold standard method to measure renal function is recommended.
Interference Alignment for Cognitive Radio Communications and Networks: A Survey
Authors: Abdulkadir Yusuf , Simpson Oluyomi , Sun Yichuang
Published: September 2019

Interference alignment (IA) is an innovative wireless transmission strategy that has shown to be a promising technique for achieving optimal capacity scaling of a multiuser interference channel at asymptotically high-signal-to-noise ratio (SNR). Transmitters exploit the availability of multiple signaling dimensions in order to align their mutual interference at the receivers. Most of the research has focused on developing algorithms for determining alignment solutions as well as proving interference alignment’s theoretical ability to achieve the maximum degrees of freedom in a wireless network. Cognitive radio, on the other hand, is a technique used to improve the utilization of the radio spectrum by opportunistically sensing and accessing unused licensed frequency spectrum, without causing harmful interference to the licensed users. With the increased deployment of wireless services, the possibility of detecting unused frequency spectrum becomes diminished. Thus, the concept of introducing interference alignment in cognitive radio has become a very attractive proposition. This paper provides a survey of the implementation of IA in cognitive radio under the main research paradigms, along with a summary and analysis of results under each system model.
Squeeze Flow of Bingham Fluids through Reticulated, Compressed Foams
Authors: Turtoi Petrică , Pascovici Mircea D. , Cicone Traian
Published: September 2019

The paper presents experimental and theoretical results for the planar squeeze flow of a finite volume of viscoplastic material through a highly deformable porous layer. The central zone of an annular disc made of a reticulated polyurethane foam with high porosity (ε > 0.97) was fully filled with tooth paste. The porous disc placed between two flat, impermeable, parallel, and rigid discs was subjected to compression and the normal force was recorded. After compression, the radial extension of the squeezed fluid was measured. The visualisation of the compressed disc managed to provide evidence of a tortuous flow inside the porous structure. An original analytical model is proposed for the prediction of the front of the flow inside the porous layer and corresponding resistant normal force. The model combines the Covey and Stanmore (1981) model for squeeze flow of a Bingham fluid inside the central zone, with an original approach for flow through the reticulated foams, based on the concept of “equivalent flow tubes” with variable tortuosity. This explorative investigation is of interest for innovative shock absorbers. The model validity covers both low and high plasticity numbers and was experimentally validated for low speed.
A Comparative 3D Finite Element Computational Study of Three Connections
Authors: Farronato Davide , Manfredini Mattia , Stevanello Andrea , Campana Veronica , Azzi Lorenzo , Farronato Marco
Published: September 2019

Masticatory overload on dental implants is one of the causes of marginal bone resorption. The implant–abutment connection (IAC) design plays a critical role in the quality of the stress distribution, and, over the years, different designs were proposed. This study aimed to assess the mechanical behavior of three different types of IAC using a finite element model (FEM) analysis. Three types of two-piece implants were designed: two internal conical connection designs (models A and B) and one internal flat-to-flat connection design (model C). This three-dimensional analysis evaluated the response to static forces on the three models. The strain map, stress analysis, and safety factor were assessed by means of the FEM examination. The FEM analysis indicated that forces are transmitted on the abutment and implant’s neck in model B. In models A and C, forces were distributed along the internal screw, abutment areas, and implant’s neck. The stress distribution in model B showed a more homogeneous pattern, such that the peak forces were reduced. The conical shape of the head of the internal screw in model B seems to have a keystone role in transferring the forces at the surrounding structures. Further experiments should be carried out in order to confirm the present suppositions.
Influence of Polyurea Composite Coating on Selected Mechanical Properties of AISI 304 Steel
Authors: Duda Monika , Pach Joanna , Lesiuk Grzegorz
Published: September 2019

This paper contains experimental results of mechanical testing of the AISI 304 steel with composite coatings. The main goal was to investigate the impact of the applied polyurea composite coating on selected mechanical properties: Adhesion, impact resistance, static behavior, and, finally, fatigue lifetime of notched specimens. In the paper the following configurations of coatings were tested: EP (epoxy resin), EP_GF (epoxy resin + glass fabric), EP_GF_HF (epoxy resin + glass fabric hemp fiber), EP_PUA (epoxy resin + polyurea) resin, EP_GF_PUA (epoxy resin + glass fabric + polyurea) resin, and EP_GF_HF_PUA (epoxy resin + glass fabric + hemp fiber + polyurea) resin. The highest value of force required to break adhesive bonds was observed for the EP_PUA coating, the smallest for the single EP coating. A tendency of polyurea to increase the adhesion of the coating to the base was noticed. The largest area of delamination during the impact test was observed for the EP_GF_HF coating and the smallest for the EP-coated sample. In all tested samples, observed delamination damage during the pull-off test was located between the coating and the metallic base of the sample.
Discrete Element Mesoscale Modeling of Recycled Lump Concrete under Axial Compression
Authors: Yu Yong , Wu Bo
Published: September 2019

In the past decade, directly reusing large pieces of coarsely crushed concrete (referred to as demolished concrete lumps or DCLs) with fresh concrete in new construction was demonstrated as an efficient technique for the recycling of waste concrete. Previous studies investigated the mechanical properties of recycled lump concrete (RLC) containing different sizes of DCLs; however, for actual application of this kind of concrete, little information is known about the influence of the spatial locations of DCLs and coarse aggregates on the concrete strength. Moreover, the mechanical responses of such a concrete containing various shapes of DCLs are also not well illustrated. To add knowledge related to these topics, two-dimensional mesoscale simulations of RLC containing DCLs under axial compression were performed using the discrete element method. The main variables of interest were the relative strength of the new and old concrete, the distribution of the lumps and other coarse aggregates, and the shape of the lumps. In addition, the differences in compression behavior between RLC and recycled aggregate concrete were also predicted. The numerical results indicate that the influence tendency of the spatial locations of DCLs and coarse aggregate pieces on the compressive stress–strain curves for RLC is similar to that of the locations of coarse aggregates for ordinary concrete. The strength variability of RLC is generally higher than that of ordinary concrete, regardless of the relative strength of the new and old concrete included; however, variability has no monotonic trend with an increase in the lump replacement ratio. The mechanical properties of RLC in compression are little influenced by the geometric shape of DCLs as long as the ratio of the length of their long axis to short axis is smaller than 2.0. The compressive strength and elastic modulus of RLC are always superior to those of recycled aggregate concrete designed with a conventional mixing method.
Enhancement of Photorefraction in Vanadium-Doped Lithium Niobate through Iron and Zirconium Co-Doping
Authors: Saeed Shahzad , Liu Hongde , Xue Liyun , Zheng Dahuai , Liu Shiguo , Chen Shaolin , Kong Yongfa , Rupp Romano , Xu Jingjun
Published: September 2019

A series of mono-, double-, and tri-doped LiNbO3 crystals with vanadium were grown by Czochralski method, and their photorefractive properties were investigated. The response time for 0.1 mol% vanadium, 4.0 mol% zirconium, and 0.03 wt.% iron co-doped lithium niobate crystal at 488 nm was shortened to 0.53 s, which is three orders of magnitude shorter than the mono-iron-doped lithium niobate, with a maintained high diffraction efficiency of 57% and an excellent sensitivity of 9.2 cm/J. The Ultraviolet-visible (UV-Vis) and OH− absorption spectra were studied for all crystals tested. The defect structure is discussed, and a defect energy level diagram is proposed. The results show that vanadium, zirconium, and iron co-doped lithium niobate crystals with fast response and a moderately large diffraction efficiency can become another good candidate material for 3D-holographic storage and dynamic holography applications.
A Stochastic Damage Model for Bond Stress-Slip Relationship of Rebar-Concrete Interface under Monotonic Loading
Authors: Lv Xiaoyong , Yu Zhiwu , Shan Zhi , Yuan Ju
Published: September 2019

The stochastic bond stress-slip behavior is an essential topic for the rebar-concrete interface. However, few theoretical models incorporating stochastic behavior in current literature can be traced. In this paper, a stochastic damage model based on micro-mechanical approach for bond stress-slip relationship of the interface under monotonic loading was proposed. In order to describe the mechanical behaviors of the rebar-concrete interface, a microscopic damage model was proposed. By introducing a micro-element consists of parallel spring element, friction element and a switch element, the model is formulated. In order to reflect the randomness of the bond stress-slip behavior contributed by the micro-fracture in the interface, a series of paralleled micro-elements are adopted with the failure threshold of individual spring element is set as a random variable. The expression of both mean and variance for the bond stress-slip relationship was derived based on statistical damage mechanics. Furthermore, by utilizing a search heuristic global optimization algorithm (i.e., a genetic algorithm), parameters of the proposed model are able to be identified from experimental results, which a lognormal distribution has adopted. The prediction was verified against experimental results, and it reveals that the proposed model is capable of capturing the random nature of the micro-structure and characterizing the stochastic behavior.
The Effect of the Static Load in the UNSM Process on the Corrosion Properties of Alloy 600
Authors: Kim Ki Tae , Kim Young Sik
Published: September 2019

To suppress stress corrosion-cracking, compressive residual stresses, such as shot peening, laser peening, water jet peening, ultrasonic peening, and ultrasonic nanocrystal surface modification (UNSM) are utilized. However, among the numerous techniques, there is little research about the corrosion effect of detailed conditions, such as static load or amplitude in UNSM. A study on UNSM among various techniques of adding compressive residual stress to Alloy 600 was conducted. The focus of this study was on the effect of the static load in UNSM on the corrosion properties of Alloy 600. Microstructure analysis was conducted using an optical microscope (OM), a scanning electron microscope (SEM), and electron backscattering diffraction (EBSD), while compressive residual stress was measured using a nano-indentation technique. A cyclic polarization test and the AC (Alternating Current)-impedance measurement were both used to analyze the corrosion properties. An increase in static load under critical static load enhanced the grain boundary diffusion, consequently strengthened the passive film, and facilitated the surface diffusion, thereby improving the passivation of Alloy 600. However, higher static loads over the critical value can lead to an increase in the friction between the striking tip and the surface, thereby creating an overlapped wave, which reduces the corrosion properties.
Measurement of Restitution and Friction Coefficients for Granular Particles and Discrete Element Simulation for the Tests of Glass Beads
Authors: Tang Hongxiang , Song Rui , Dong Yan , Song Xiaoyu
Published: September 2019

A slant plate flat throw test system for measuring the restitution coefficient of granular materials and a sliding friction test instrument for measuring the friction coefficient between discrete particles and continuum boundary surface materials are developed. The restitution coefficients of the glass bead particles, the glass beads relative to the glass plate, the composite of glass plate and the rubber membrane and the friction coefficients between the glass beads and the rubber film and the filter paper are measured by the designed methods. Based on the measured restitution coefficient and friction coefficient, the discrete element numerical simulation is carried out for triaxial test and plane strain test. Through comparing the experimental results and the discrete element numerical simulation results, the feasibility and rationality of the designed measurement methods and the discrete element numerical simulation are verified. The measuring methods developed in this paper can be further applied to the tests of other fine particles.
Elemental Silver Nanoparticles: Biosynthesis and Bio Applications
Authors: Mikhailov Oleg V. , Mikhailova Ekaterina O.
Published: September 2019

The data on the specifics of synthesis of elemental silver nanoparticles (Ag-NP) having various geometric shapes (pseudo spherical, prismatic, cubic, trigonal-pyramidal, etc.), obtained by using various biological methods, and their use in biology and medicine have been systematized and generalized. The review covers mainly publications published in the current 21st century. Bibliography: 262 references.
Forming Features and Properties of Titanium Alloy Billets After Radial-Shear Rolling
Authors: Skripalenko Mikhail Mikhailovich , Galkin Sergei Pavlovich , Karpov Boris Vladimirovich , Romantsev Boris Alekseevich , Kaputkina Lyudmila Mikhailovna , Danilin Andrei Vladimirovich , Skripalenko Mikhail Nikolaevich , Patrin Pavel Vladimirovich
Published: September 2019

Radial-shear rolling (RSR) of titanium alloy billets was realized in a three-high rolling mill. Experimental rolling was simulated using DEFORM software. The purpose was to reveal how stress-strain state parameters, grain structure and hardness vary along the billet’s radius in the stationary stage of the RSR process. It was also the goal to establish a relation between stress state parameters, hardness and grain structure. Changes in the accumulated strain and the stress triaxiality were established by computer simulation. Hardness and grain size changes were obtained after experimental rolling. The novelty aspect is that both computer simulation and experimental rolling showed that there is a ring-shape area with lowered strength in the billet’s cross-section. The radius of the ring-shape area was predicted as a result of the research.
Aluminum-Alumina Composites: Part I: Obtaining and Characterization of Powders
Authors: Gromov Alexander A. , Nalivaiko Anton Yu. , Ambaryan Grayr N. , Vlaskin Mikhail S. , Buryakovskaya Olesya A. , Kislenko Sergey A. , Zhuk Andrey Z. , Shkolnikov Evgeniy I. , Slyusarskiy Konstantin V. , Osipenkova Alexandra A. , Arnautov Alexey N.
Published: September 2019

The process of advanced aluminum-alumina powders production for selective laser melting was studied. The economically effective method of obtaining aluminum-alumina powdery composites for further selective laser melting was comprehensively studied. The aluminum powders with 10–20 wt. % alumina content were obtained by oxidation of aluminum in water. Aluminum oxidation was carried out at ≤200 °C. The oxidized powders were further dried at 120 °C and calcined at 600 °C. Four oxidation modes with different process temperatures (120–200 °C) and pressures (0.15–1.80 MPa) were investigated. Parameters of aluminum powders oxidation to obtain composites with 10.0, 14.5, 17.4, and 20.0 wt. % alumina have been determined. The alumina content, particle morphology, and particle size distribution for the obtained aluminum-alumina powdery composites were studied by XRD, SEM, laser diffraction, and volumetric methods. According to the obtained characteristics of aluminum-alumina powdery composites, they are suitable for the SLM process.
On the Non-Hypercyclicity of Normal Operators, Their Exponentials, and Symmetric Operators
Authors: Markin Marat V. , Sichel Edward S.
Published: September 2019

We give a simple, straightforward proof of the non-hypercyclicity of an arbitrary (bounded or not) normal operator A in a complex Hilbert space as well as of the collection e t A t ≥ 0 of its exponentials, which, under a certain condition on the spectrum of A, coincides with the C 0 -semigroup generated by it. We also establish non-hypercyclicity for symmetric operators.
Antrodia Cinnamomea Prolongs Survival in a Patient with Small Cell Lung Cancer
Authors: Long Huei , Hu Chi-Tan , Weng Ching-Feng
Published: September 2019

Introduction: Antrodia cinnamomea (AC) is an extremely rare medicinal fungus native to forested regions of Taiwan. It possesses numerous biological activities, especially anti-tumor effects shown in various in vitro cancer cells and in vivo animal models. However, there are few clinical reports about AC as a treatment for cancer patients. This report attempts to demonstrate the therapeutic effect of dish-cultured AC (DAC) on a small cell lung cancer (SCLC) patient taken orally for an extended duration. Patient concerns: An 88-year-old male with a history of diabetes mellitus and hypertension visited the outpatient department with the symptoms of dyspnea and a cough for two weeks. After a diagnosis of SCLC, the patient declined both chemotherapy and radiotherapy because of the side effects and only accepted supportive care without additional therapy. Diagnosis: Limited-stage SCLC (T4N2M1a, stage IV) after the chest radiograph, computed tomography-guided biopsy, and pathological diagnosis. Interventions: The patient was prescribed DAC with an increasing dosage, from 5 g/d up to 10 g/d DAC, for six months, without radiation or chemotherapy treatment. Outcomes: DAC caused the tumor to shrink substantially. Surprisingly, the patient survived for 32 months without relapse after six months of DAC treatment. Laboratory examinations indicated that the patient’s health had improved significantly, reverting to near normal levels. Notably, he had a good quality of life with a high Barthel index score. Unfortunately, this patient died of septic shock caused by acute cholangitis. Conclusion: DAC may exert an anti-cancer effect, which can lead to tumor regression. This is supposed to be achieved by the combined DAC’s immunomodulatory, anti-angiogenic, anti-metastatic, anti-proliferative, and pro-apoptotic effects mediated through multiple signaling pathways. We propose that DAC can be used as a complementary medicine to prolong the life expectancy and improve the life quality of SCLC patients.
Pollen-Food Allergy Syndrome: A not so Rare Disease in Childhood
Authors: Mastrorilli Carla , Cardinale Fabio , Giannetti Arianna , Caffarelli Carlo
Published: September 2019

Seasonal allergic rhinoconjunctivitis (SAR) affects millions of people worldwide, particularly in childhood and adolescence. Pollen food allergy syndrome (PFAS) is a common adverse reaction occurring few minutes after the consumption of vegetable foods in patients with pollen-induced SAR. PFAS has rarely been investigated in the pediatric population, as it has been mainly examined as an adult disease. Recent studies suggested that PFAS might be more frequent in childhood than previously recognized. The present review aims to give an overview of the epidemiology, pathophysiology, diagnosis, management and prognosis of PFAS in children with SAR-induced by pollens.
Effect of Quinolones Versus Cefixime on International Normalized Ratio Levels After Valve Replacement Surgery with Warfarin Therapy
Authors: Liaqat Anam , Khan Arif-ullah , Asad Muhammad , Khalil Hafsa
Published: September 2019

Background and Objectives: A dispute over interaction of warfarin with two quinolones—i.e., moxifloxacin and levofloxacin—leading to significant increase in international normalized ratio (INR) levels and coagulopathies is currently in debate. The study objective was to compare the INR values due to addition of quinolones and cefixime in warfarin treated patients after replacement of disease valves with metallic valves. Material and Methods: A prospective evaluation of patients who undergone valve replacement surgeries in the cardiology hospital setup in Pakistan during the period 2018–2019 was done, including all those subjects treated concurrently with levofloxacin, moxifloxacin, cefixime, and warfarin for the study. Data organized included demographic information, concurrent medications, and appropriate analytical parameters, especially INR values taken before and within seven days after prescribing three antibiotics in discharged patients who had undergone valve replacement surgeries. Patients for whom laboratory INR values were not given at the time of discharge and with deranged liver function, renal function, low albumin levels, and febrile patients were removed from study. Furthermore, patients were advised on possible food interactions and evaluated to examine if these factors have any possible influence on the interaction being studied. Results: Differences in INR were analyzed statistically by means of SPSS analysis before and after the possible interaction. Following the administration of levofloxacin and moxifloxacin to warfarin therapy, statistical analysis showed remarkable increase in INR (p < 0.001) and no significant change in INR was observed after cefixime treatment (p > 0.05). Conclusion: Results showed that, after adding levofloxacin and moxifloxacin in patients on warfarin, therapy contributed to remarkable increase in INR. However, addition of cefixime prevented frequent coagulopathies; therefore, close monitoring of INR and switching to a safe antibiotic such as cefixime is recommended.
Racial and Gender Differences in the Presentation of Pruritus
Authors: Whang Katherine A. , Khanna Raveena , Thomas Jamael , Aguh Crystal , Kwatra Shawn G.
Published: September 2019

Background: Pruritus is a common disease symptom with a variety of etiologies known to reduce patient quality of life. We aimed to characterize the racial and gender differences in the presentation of pruritus for itch-related patient visits both within a single institution and nationally. Methods: Cross sectional study of patients ≥ 18 years old seen at Johns Hopkins Health System between 1/1/12 and 1/1/18. Results were compared to data from 2005–2011 from the National Ambulatory Medical Care Survey (NAMCS) and the National Health Ambulatory Medical Care Survey (NHAMCS). Results: Our findings indicate that itch patients at JHHS (n = 18,753) were more likely to be black compared to white patients (37% vs. 19%, p < 0.01) when compared to patients without itch—a trend also noted nationally based on data from NAMCS/NHAMCS (26% vs. 21%, p = 0.05). Black itch patients are also more likely to be diagnosed with prurigo nodularis (OR 2.37, p < 0.0001), lichen planus (OR 1.22, p < 0.0001), and atopic dermatitis OR 1.51, p < 0.0001). Female itch patients are more likely to be diagnosed with autoimmune (OR 1.66, p < 0.0001) and psychiatric comorbidities (OR 1.2–1.8, p < 0.0001) than male itch patients. When compared to black itch patients nationally, white itch patients were more likely to visit a dermatologist (29% vs. 18%, p = 0.028). Our data can identify associated conditions and demographic differences but are unable to support a causal relationship. Conclusions: Black and female patients are more likely to present with pruritus, a symptom associated with comorbidities such as prurigo nodularis, lichen planus, atopic dermatitis, and psychiatric conditions.