bluuebunny/crossref_metadata_2025 huggingface dataset:
How Decisive Are the Copenhagen Criteria for EU Enlargement?
Authors: Nádasi Levente , Trón Zsuzsanna
Published: March 2024

The article analyses the significance of the accession criteria in the context of recent EU enlargement negotiations, particularly during heightened geopolitical circumstances triggered by Russia’s aggression against Ukraine. The accelerated EU enlargement process, evidenced by the swift membership applications from Ukraine, Moldova and Georgia, and the even quicker decision to open accession negotiations for two of them, inspires an exploration of the binding elements, specifically the Copenhagen criteria, integral to the accession negotiation process. The study aims to examine the numerical development and changes in the conditions and criteria necessary for EU membership in ten candidate and potential candidate countries. The analysis focuses on fulfilling essential conditions, with emphasis on convergence, assessed using GDP per capita and growth rates, as well as the rule of law and democracy criteria measured through the Worldwide Governance Indicators.
Bosnia and Herzegovina’s Hopes of European Union Accession: A Safeguard against the Country’s Implosion?
Authors: Kundid Maxime
Published: March 2024

The prospect of Bosnia and Herzegovina’s accession to the European Union encapsulates a pivotal chapter in the nation’s trajectory. As the country navigates the intricate path toward EU membership, it faces a myriad of challenges and opportunities that extend beyond mere geopolitical realignment. This journey involves addressing complex issues such as political stability, governance reforms, rule of law and economic development. The significance of this process lies not only in its potential to reshape Bosnia and Herzegovina’s political and economic landscape but also in the broader implications for regional stability and the consolidation of European values in the Western Balkans. In this context, the journey towards EU accession for Bosnia and Herzegovina is a multifaceted endeavour, reflecting both the aspirations and complexities inherent in the pursuit of a European future.
THE ESSENCE OF DOUBLE TAXATION IN TRANSFER PRICING
Authors:   State Tax University, Kraievskyi Volodymyr , Myskin Yurii ,   State Tax University
Published: March 2024

The article is devoted to the study of the essence and nature of double taxation in the conditions of transfer pricing. The crux of the problem is that profits from transfer transactions may be subject to taxation in both countries where the related parties are located. This can create complications and misunderstandings between countries, as well as cause conflicts between businesses and tax authorities. The main goal of solving the problem of double taxation in transfer pricing is to create a stable and transparent system that satisfies the interests of all parties. International standards and dispute resolution mechanisms play a key role in achieving this goal, ensuring a fair and clear playing field for all participants. In the further development of transfer pricing and the fight against double taxation, it is important to continue the development and implementation of effective international standards, as well as to strengthen cooperation between countries and enterprises. Only in conditions of mutual understanding and cooperation will it be possible to ensure an effective and fair transfer pricing system that considers the interests of all parties.
Update on the Vein of Galen Aneurysmal Malformation : Disease Concept and Genetics
Authors: Kang Hyun-Seung
Published: March 2024

Vein of Galen aneurysmal malformation is one of important pediatric arteriovenous shunt diseases, especially among neonates and infants. Here, early history of the disease identification, basic pathoanatomy with a focus on the embryonic median prosencephalic vein, classification and differential diagnoses, and recent genetic studies are reviewed.
Perancangan Logo dan Desain Kemasan Produk Minuman Olahan Susu Merek Ibu Yudhi untuk Usia 6-12 Tahun dalam Upaya Meningkatkan Penjualan Produk di Sidoarjo
Authors: PP Bintang Pramudya , Sukmaraga Ayyub Anshari
Published: March 2024

UMKM yang ada di Indonesia tidak dapat dianggap sebelah mata. UMKM dapat menjadi perekonomian terbesar di Indonesia. Untuk itu diperlukannya tingkat kesadaran tinggi untuk memiliki merek atau identitas perusahaan agar dapat lebih berkermbang. Maka dari itu perlu dibuat logo dan desain kemasan pada produk sebagai identitas dari perusahaan. Tujuannya adalah untuk pembeda antara produk satu dengan yang lain serta dapat membuat perusaahan dari satu produk menjadi lebih dikenal oleh masyarakat. Dalam penelitian ini metode yang digunakan adalah metode kualitatif dengan mengumpulkan data secara observasi, beserta wawancara dengan pemilik perusahaan dan studi pustaka. Media pendukung dalamperancangan ini adalah gantungan kunci, tas belanja, tas serut, small note, stiker, botol minum,topi, display, kotak pensil.
Geoscience Job Advertisements as a Barrier to Employment for People With Disabilities
Authors: Shafer G. W. , Viskupic K. , Egger A. E.
Published: March 2024

Individuals with physical disabilities are largely underrepresented in the geoscience workforce. In this study, we analyzed over 2,500 job advertisements (ads) for entry-level geoscience positions across 19 industries to assess how inclusive the United States job market is for people with physical disabilities. We evaluated each ad’s Equal Opportunity Employer (EEO) and accommodation statements to create a measure of geoscience employers’ inclusive practices for people with disabilities. We coded each ad for instances where physical abilities (e.g., traversing rough terrain, driving a vehicle, lifting heavy objects) were listed as required or preferred qualifications and whether these abilities matched the core job function. A significant proportion of job ads (44%) did not include EEO statements, and of those that did, the language used was minimal or abbreviated. Additionally, only 18% of ads mentioned accommodations for people with disabilities. Of the ads that required physical abilities, only 19% requested physical abilities that matched the core job function. Students exploring their career options or applying for entry-level jobs may feel disadvantaged, restrict their applications, or dismiss geoscience careers if they have physical limitations, or if they perceive that the work environment is not inclusive. Overall, online geoscience ads could benefit from adding or modifying equal opportunity employment and accommodations statements to reflect a more inclusive workplace and could explicitly link requested physical abilities to the job description. These results could help employers consider possible modifications to their job advertisements and explore alternative strategies to promote a more inclusive geoscience workforce.
Recent development and future application of biodegradable ureteral stents
Authors: Hu Ke , Hou Zhipeng , Huang Yuanbin , Li Xueying , Li Xiancheng , Yang Liqun
Published: March 2024

Ureteral stenting is a common clinical procedure for the treatment of upper urinary tract disorders, including conditions such as urinary tract infections, tumors, stones, and inflammation. Maintaining normal renal function by preventing and treating ureteral obstruction is the primary goal of this procedure. However, the use of ureteral stents is associated with adverse effects, including surface crusting, bacterial adhesion, and lower urinary tract symptoms (LUTS) after implantation. Recognizing the need to reduce the complications associated with permanent ureteral stent placement, there is a growing interest among both physicians and patients in the use of biodegradable ureteral stents (BUS). The evolution of stent materials and the exploration of different stent coatings have given these devices different roles tailored to different clinical needs, including anticolithic, antibacterial, antitumor, antinociceptive, and others. This review examines recent advances in BUS within the last 5 years, providing an in-depth analysis of their characteristics and performance. In addition, we present prospective insights into the future applications of BUS in clinical settings.
ECM and epithelial stem cells: the scaffold of destiny
Authors: Estrach Soline , Vivier Charles-Maxime , Féral Chloé C.
Published: March 2024

Adult stem cells play a critical role in maintaining tissue homeostasis and promoting longevity. The intricate organization and presence of common markers among adult epithelial stem cells in the intestine, lung, and skin serve as hallmarks of these cells. The specific location pattern of these cells within their respective organs highlights the significance of the niche in which they reside. The extracellular matrix (ECM) not only provides physical support but also acts as a reservoir for various biochemical and biophysical signals. We will consider differences in proliferation, repair, and regenerative capacities of the three epithelia and review how environmental cues emerging from the niche regulate cell fate. These cues are transduced via mechanosignaling, regulating gene expression, and bring us to the concept of the fate scaffold. Understanding both the analogies and discrepancies in the mechanisms that govern stem cell fate in various organs can offer valuable insights for rejuvenation therapy and tissue engineering.
Maternal nutrient metabolism in the liver during pregnancy
Authors: Fang Hongxu , Li Qingyang , Wang Haichao , Ren Ying , Zhang Leying , Yang Ling
Published: March 2024

The liver plays pivotal roles in nutrient metabolism, and correct hepatic adaptations are required in maternal nutrient metabolism during pregnancy. In this review, hepatic nutrient metabolism, including glucose metabolism, lipid and cholesterol metabolism, and protein and amino acid metabolism, is first addressed. In addition, recent progress on maternal hepatic adaptations in nutrient metabolism during pregnancy is discussed. Finally, the factors that regulate hepatic nutrient metabolism during pregnancy are highlighted, and the factors include follicle-stimulating hormone, estrogen, progesterone, insulin-like growth factor 1, prostaglandins fibroblast growth factor 21, serotonin, growth hormone, adrenocorticotropic hormone, prolactin, thyroid stimulating hormone, melatonin, adrenal hormone, leptin, glucagon-like peptide-1, insulin glucagon and thyroid hormone. Our vision is that more attention should be paid to liver nutrient metabolism during pregnancy, which will be helpful for utilizing nutrient appropriately and efficiently, and avoiding liver diseases during pregnancy.
Application of machine learning in ultrasonic diagnostics for prismatic lithium-ion battery degradation evaluation
Authors: Wang Qiying , Song Da , Lin Xingyang , Wu Hanghui , Shen Hang
Published: March 2024

Lithium-ion batteries are essential for electrochemical energy storage, yet they undergo progressive aging during operational lifespan. Consequently, precise estimation of their state of health (SOH) is crucial for effective and safe operation of energy storage systems. This paper investigates the viability of ultrasound-based methods for assessing the SOH of prismatic lithium-ion batteries. In the experimental framework, a designated prismatic lithium-ion battery was subjected to numerous charging and discharging cycles using a battery cycling system. Subsequently, ultrasonic detection experiments were conducted to record the waveforms of the transmitted and received signals. These signals were then processed through wavelet transforms to extract signal amplitude and time-of-flight data. To analyse these data, we applied four algorithms: linear regression, support vector machines, Gaussian process regression, and neural networks. The predictive performance of each algorithm was evaluated through extensive experimentation and analysis. The combination of ultrasonic signals with computational models has emerged as a robust technique for precise battery degradation assessment, suggesting its potential as a standard in battery health evaluation methods.
Automated recognition of chromosome fusion using an alignment-free natural vector method
Authors: Yu Hongyu , Yau Stephen S.-T.
Published: March 2024

Chromosomal fusion is a significant form of structural variation, but research into algorithms for its identification has been limited. Most existing methods rely on synteny analysis, which necessitates manual annotations and always involves inefficient sequence alignments. In this paper, we present a novel alignment-free algorithm for chromosomal fusion recognition. Our method transforms the problem into a series of assignment problems using natural vectors and efficiently solves them with the Kuhn-Munkres algorithm. When applied to the human/gorilla and swamp buffalo/river buffalo datasets, our algorithm successfully and efficiently identifies chromosomal fusion events. Notably, our approach offers several advantages, including higher processing speeds by eliminating time-consuming alignments and removing the need for manual annotations. By an alignment-free perspective, our algorithm initially considers entire chromosomes instead of fragments to identify chromosomal structural variations, offering substantial potential to advance research in this field.
Exploring secretory proteome and cytokine kinetic of human peripheral blood mononuclear cells exposed to methicillin-resistant Staphylococcus aureus biofilms and planktonic bacteria
Authors: Gheitasi Reza , Röll Daniela , Müller Mario M. , Naseri Mohadeseh , König Rainer , Slevogt Hortense , Pletz Mathias W. , Makarewicz Oliwia
Published: March 2024

Staphylococcus aureus is a highly successful pathogen infecting various body parts and forming biofilms on natural and artificial surfaces resulting in difficult-to-treat and chronic infections. We investigated the secreted cytokines and proteomes of isolated peripheral blood mononuclear cells (PBMCs) from healthy volunteers exposed to methicillin-resistant S. aureus (MRSA) biofilms or planktonic bacteria. Additionally, the cytokine profiles in sera from patients with community-acquired pneumonia (CAP) caused by S. aureus were investigated. The aim was to gain insights into the immune response involved and differentiate between the planktonic and sessile MRSA forms. We identified 321 and 298 targets that were significantly differently expressed in PBMCs when exposed to planktonic or biofilm-embedded bacteria, respectively. PBMCs exposed to planktonic MRSA cells secreted increased levels of TNF-α, while IL-18 was elevated when exposed to the biofilm. The machine-learning analyses of the cytokine profiles obtained for the in vitro PBMCs and CAP sera distinguished between the two types of bacteria forms based on cytokines IL-18, IL12, and IL-17, and with a lower importance IL-6. Particularly, IL-18 which has not been correlated with S. aureus biofilms so far might represent a suitable marker for monitoring chronification during MRSA infection to individualize the therapy, but this hypothesis must be proved in clinical trials.
Leaky gut, circulating immune complexes, arthralgia, and arthritis in IBD: coincidence or inevitability?
Authors: Jin Xi-ya , Li Dan-dan , Quan Wei , Chao Yang , Zhang Bin
Published: March 2024

Most host-microbiota interactions occur within the intestinal barrier, which is essential for separating the intestinal epithelium from toxins, microorganisms, and antigens in the gut lumen. Gut inflammation allows pathogenic bacteria to enter the blood stream, forming immune complexes which may deposit on organs. Despite increased circulating immune complexes (CICs) in patients with inflammatory bowel disease (IBD) and discussions among IBD experts regarding their potential pathogenic role in extra-intestinal manifestations, this phenomenon is overlooked because definitive evidence demonstrating CIC-induced extra-intestinal manifestations in IBD animal models is lacking. However, clinical observations of elevated CICs in newly diagnosed, untreated patients with IBD have reignited research into their potential pathogenic implications. Musculoskeletal symptoms are the most prevalent extra-intestinal IBD manifestations. CICs are pivotal in various arthritis forms, including reactive, rheumatoid, and Lyme arthritis and systemic lupus erythematosus. Research indicates that intestinal barrier restoration during the pre-phase of arthritis could inhibit arthritis development. In the absence of animal models supporting extra-intestinal IBD manifestations, this paper aims to comprehensively explore the relationship between CICs and arthritis onset via a multifaceted analysis to offer a fresh perspective for further investigation and provide novel insights into the interplay between CICs and arthritis development in IBD.
Identification of two flavonoids antiviral inhibitors targeting 3C-like protease of porcine epidemic diarrhea virus
Authors: Li Zhonghua , Zhu Liangyun , Wang Lu , Huang Yizhi , Zhang Yi , Zhao Di , Wang Lei , Yi Dan , Hou Yongqing , Wu Tao
Published: March 2024

Porcine epidemic diarrhea virus (PEDV) has caused severe damage to the global pig industry in the past 20 years, creating an urgent demand for the development of associated medications. Flavonoids have emerged as promising candidates for combating coronaviruses. It is believed that certain flavonoids can directly inhibit the 3C-like protease (3CLpro), thus displaying antiviral activity against coronaviruses. In this investigation, we applied a flavonoid library to screen for natural compounds against PEDV 3CLpro. Baicalein and baicalin were found to efficiently inhibit PEDV 3CLproin vitro, with the IC50 value of 9.50 ± 1.02 μM and 65.80 ± 6.57 μM, respectively. A docking analysis supported that baicalein and baicalin might bind to the active site and binding pocket of PEDV 3CLpro. Moreover, both baicalein and baicalin successfully suppressed PEDV replication in Vero and LLC-PK1 cells, as indicated by reductions in viral RNA, protein, and titer. Further investigation revealed that baicalein and baicalin mainly inhibited the early viral replication of the post-entry stage. Furthermore, baicalein showed potential effects on the attachment or invasion step of PEDV. Collectively, our findings provide experimental proof for the inhibitory effects of baicalein and baicalin on PEDV 3CLpro activity and PEDV infection. These discoveries may introduce novel therapeutic strategies for controlling porcine epidemic diarrhea (PED).
Deferiprone–resveratrol hybrid attenuates iron accumulation, oxidative stress, and antioxidant defenses in iron-loaded human Huh7 hepatic cells
Authors: Li Jin , Koonyosying Pimpisid , Korsieporn Woranontee , Paradee Narisara , Hutachok Nuntouchaporn , Xu Honghong , Ma Yongmin , Chuljerm Hataichanok , Srichairatanakool Somdet
Published: March 2024

Chronic liver diseases are complications of thalassemia with iron overload. Iron chelators are required to remove excessive iron, and antioxidants are supplemented to diminish harmful reactive oxygen species (ROS), purposing to ameliorate oxidative liver damage and dysfunctions. The deferiprone–resveratrol hybrid (DFP–RVT) is a synthetic iron chelator possessing anti-β-amyloid peptide aggregation, anti-malarial activity, and hepatoprotection in plasmodium-infected mice. The study focuses on investigating the antioxidant, cytotoxicity, iron-chelating, anti-lipid peroxidation, and antioxidant defense properties of DFP–RVT in iron-loaded human hepatocellular carcinoma (Huh7) cells. In the findings, DFP–RVT dose dependently bound Fe(II) and Fe(III) and exerted stronger ABTS•- and DPPH•-scavenging (IC50 = 8.0 and 164 μM, respectively) and anti-RBC hemolytic activities (IC50 = 640 μM) than DFP but weaker than RVT (p < 0.01). DFP–RVT was neither toxic to Huh7 cells nor PBMCs. In addition, DFP–RVT diminished the level of redox-active iron (p < 0.01) and decreased the non-heme iron content (p < 0.01) in iron-loaded Huh7 cells effectively when compared without treatment in the order of DFP–RVT > RVT ∼ DFP treatments (50 µM each). Moreover, the compound decreased levels of hepatic ROS in a dose-dependent manner and the level of malondialdehyde, which was stronger than DFP but weaker than RVT. Furthermore, DFP–RVT restored the decrease in the GSH content and GPX and SOD activities (p < 0.01) in iron-loaded Huh7 cells in the dose-dependent manner, consistently in the order of RVT > DFP–RVT > DFP. Thus, the DFP–RVT hybrid possesses potent iron chelation, antioxidation, anti-lipid peroxidation, and antioxidant defense against oxidative liver damage under iron overload.
A data-driven acceleration-level scheme for image-based visual servoing of manipulators with unknown structure
Authors: Wen Liuyi , Xie Zhengtai
Published: March 2024

The research on acceleration-level visual servoing of manipulators is crucial yet insufficient, which restricts the potential application range of visual servoing. To address this issue, this paper proposes a quadratic programming-based acceleration-level image-based visual servoing (AIVS) scheme, which considers joint constraints. Besides, aiming to address the unknown problems in visual servoing systems, a data-driven learning algorithm is proposed to facilitate estimating structural information. Building upon this foundation, a data-driven acceleration-level image-based visual servoing (DAIVS) scheme is proposed, integrating learning and control capabilities. Subsequently, a recurrent neural network (RNN) is developed to tackle the DAIVS scheme, followed by theoretical analyses substantiating its stability. Afterwards, simulations and experiments on a Franka Emika Panda manipulator with eye-in-hand structure and comparisons among the existing methods are provided. The obtained results demonstrate the feasibility and practicality of the proposed schemes and highlight the superior learning and control ability of the proposed RNN. This method is particularly well-suited for visual servoing applications of manipulators with unknown structure.
A rotary transformer cross-subject model for continuous estimation of finger joints kinematics and a transfer learning approach for new subjects
Authors: Lin Chuang , He Zheng
Published: March 2024

Introduction

Surface Electromyographic (sEMG) signals are widely utilized for estimating finger kinematics continuously in human-machine interfaces (HMI), and deep learning approaches are crucial in constructing the models. At present, most models are extracted on specific subjects and do not have cross-subject generalizability. Considering the erratic nature of sEMG signals, a model trained on a specific subject cannot be directly applied to other subjects. Therefore, in this study, we proposed a cross-subject model based on the Rotary Transformer (RoFormer) to extract features of multiple subjects for continuous estimation kinematics and extend it to new subjects by adversarial transfer learning (ATL) approach.

Methods

We utilized the new subject’s training data and an ATL approach to calibrate the cross-subject model. To improve the performance of the classic transformer network, we compare the impact of different position embeddings on model performance, including learnable absolute position embedding, Sinusoidal absolute position embedding, and Rotary Position Embedding (RoPE), and eventually selected RoPE. We conducted experiments on 10 randomly selected subjects from the NinaproDB2 dataset, using Pearson correlation coefficient (CC), normalized root mean square error (NRMSE), and coefficient of determination (R2) as performance metrics.

Results

The proposed model was compared with four other models including LSTM, TCN, Transformer, and CNN-Attention. The results demonstrated that both in cross-subject and subject-specific cases the performance of RoFormer was significantly better than the other four models. Additionally, the ATL approach improves the generalization performance of the cross-subject model better than the fine-tuning (FT) transfer learning approach.

Discussion

The findings indicate that the proposed RoFormer-based method with an ATL approach has the potential for practical applications in robot hand control and other HMI settings. The model’s superior performance suggests its suitability for continuous estimation of finger kinematics across different subjects, addressing the limitations of subject-specific models.
Assessing of case–cohort design: a case study for breast cancer patients in Xinjiang, China
Authors: Wu Mengjuan , Zhang Tao , Gao Chunjie , Zhao Ting , Wang Lei , Sun Gang
Published: March 2024

Objective

To assess the effectiveness and clinical value of case–cohort design and determine prognostic factors of breast cancer patients in Xinjiang on the basis of case–cohort design.

Methods

The survival data with different sample characteristics were simulated by using Cox proportional risk models. To evaluate the effectiveness for the case–cohort, entire cohort, and simple random sampling design by comparing the mean, coefficient of variation, etc., of covariate parameters. Furthermore, the prognostic factors of breast cancer patients in Xinjiang were determined based on case–cohort sampling designs. The models were comprehensively evaluated by likelihood ratio test, the area under the receiver operating characteristic curve (AUC), and Akaike Information Criterion (AIC).

Results

In a simulations study, the case–cohort design shows better stability and improves the estimation efficiency when the censored rate is high. In the breast cancer data, molecular subtypes, T-stage, N-stage, M-stage, types of surgery, and postoperative chemotherapy were identified as the prognostic factors of patients in Xinjiang. These models based on the different sampling designs both passed the likelihood ratio test (p<0.05). Moreover, the model constructed under the case–cohort design had better fitting effect (AIC=3,999.96) and better discrimination (AUC=0.807).

Conclusion

Simulations study confirmed the effectiveness of case–cohort design and further determined the prognostic factors of breast cancer patients in Xinjiang based on this design, which presented the practicality of case–cohort design in actual data.
Unlocking the genetic diversity and population structure of the newly introduced two-row spring European HerItage Barley collecTion (ExHIBiT)
Authors: Bernád Villő , Al-Tamimi Nadia , Langan Patrick , Gillespie Gary , Dempsey Timothy , Henchy Joey , Harty Mary , Ramsay Luke , Houston Kelly , Macaulay Malcolm , Shaw Paul D. , Raubach Sebastian , Mcdonnel Kevin P. , Russell Joanne , Waugh Robbie , Khodaeiaminjan Mortaza , Negrão Sónia
Published: March 2024

In the last century, breeding programs have traditionally favoured yield-related traits, grown under high-input conditions, resulting in a loss of genetic diversity and an increased susceptibility to stresses in crops. Thus, exploiting understudied genetic resources, that potentially harbour tolerance genes, is vital for sustainable agriculture. Northern European barley germplasm has been relatively understudied despite its key role within the malting industry. The European Heritage Barley collection (ExHIBiT) was assembled to explore the genetic diversity in European barley focusing on Northern European accessions and further address environmental pressures. ExHIBiT consists of 363 spring-barley accessions, focusing on two-row type. The collection consists of landraces (~14%), old cultivars (~18%), elite cultivars (~67%) and accessions with unknown breeding history (~1%), with 70% of the collection from Northern Europe. The population structure of the ExHIBiT collection was subdivided into three main clusters primarily based on the accession’s year of release using 26,585 informative SNPs based on 50k iSelect single nucleotide polymorphism (SNP) array data. Power analysis established a representative core collection of 230 genotypically and phenotypically diverse accessions. The effectiveness of this core collection for conducting statistical and association analysis was explored by undertaking genome-wide association studies (GWAS) using 24,876 SNPs for nine phenotypic traits, four of which were associated with SNPs. Genomic regions overlapping with previously characterised flowering genes (HvZTLb) were identified, demonstrating the utility of the ExHIBiT core collection for locating genetic regions that determine important traits. Overall, the ExHIBiT core collection represents the high level of untapped diversity within Northern European barley, providing a powerful resource for researchers and breeders to address future climate scenarios.
Cognitive, behavioral and psychiatric symptoms in patients with spinal cord injury: a scoping review
Authors: Calderone Andrea , Cardile Davide , De Luca Rosaria , Quartarone Angelo , Corallo Francesco , Calabrò Rocco Salvatore
Published: March 2024

Spinal Cord Injury (SCI) is a condition where the spinal cord is damaged and experiences partial or complete loss of motor and/or sensory function, which is typically less than normal. After SCI, patients may exhibit more severe psychiatric symptoms and experience cognitive impairments, including reduced speed and attention processing capacity, as well as difficulties with executive function and episodic memory retention. Among the behavioral and psychiatric symptoms, depression, anxiety, substance use disorder, and posttraumatic stress disorder are the most common. This review aims to investigate the cognitive, behavioral, or psychiatric symptoms of the patient with SCI and their influence on the rehabilitation process. Studies were identified from an online search of PubMed, Web of Science, Cochrane Library, and Embase databases. Studies published between 2013-2023 were selected. This review has been registered on OSF (n) 3KB2U. We have found that patients with SCI are at high risk of cognitive impairment and experience a wide range of difficulties, including tasks based on processing speed and executive function. This clinical population may experience adjustment disorders with depression and anxiety, as well as other psychiatric symptoms such as fatigue, stress, and suicidal ideation. This review has demonstrated that SCI patients may experience psychiatric symptoms and cognitive impairments that affect their functioning. At the same time, these patients may be more prone to various adjustment and mood disorders. Moreover, these two aspects may interact with each other, causing a range of symptoms, increasing the risk of hospitalization, and delaying the rehabilitation process.
Clients’ experiences and satisfaction with produce prescription programs in California: a qualitative evaluation to inform person-centered and respectful program models
Authors: Rhodes Elizabeth C. , Pérez-Escamilla Rafael , Okoli Ngozi , Hromi-Fiedler Amber , Foster Jaime , McAndrew John , Duran-Becerra Beatriz , Duffany Kathleen O’Connor
Published: March 2024

Background

Produce prescription programs have strong potential to improve food security, fruit and vegetable consumption, and health across the life course. Understanding clients’ experiences and satisfaction with produce prescription programs is critical for evaluating the person-centeredness and quality of these programs. The objectives of this study were to (1) describe client experiences and satisfaction with produce prescription programs, with an emphasis on the extent to which they felt they were treated with respect and dignity, and (2) identify recommendations for improving client experiences.

Methods

We conducted four focus group discussions with clients of produce prescription programs in two Federally Qualified Health Centers in California. We used a modified framework analysis approach and organized participants’ experiences with programs into themes.

Results

Three themes captured participants’ program experiences. First, respectful produce prescription programming encompassed interactions with individuals delivering the programs that felt respectful (e.g., program staff showing they cared about participants’ health and offering timely assistance with financial incentives) and disrespectful (e.g., not receiving prompt responses to questions about incentives), as well as aspects of program design perceived to be respectful (e.g., provision of gift cards as financial incentives, which offered privacy when purchasing produce). Second, having autonomy to use gift cards to choose their preferred fresh fruits and vegetables was viewed as a positive experience, though participants desired greater autonomy to shop at stores other than the program designated stores. Third, participants frequently discussed program usability, with some reporting that joining the programs and using the cards was easy, and others describing difficulties activating cards and using them at stores due to cashiers’ lack of awareness of the programs. Overall, participants were highly satisfied with the programs. To improve client experiences, they recommended increasing privacy (e.g., by educating cashiers on the programs so that clients do not need to explain in public what the card is for) and autonomy (e.g., allowing cards to be used at other chain or local stores).

Discussion

Our findings inform efforts to make produce prescription programs more person-centered and respectful, which in turn may increase program demand, engagement, and impact.
Exploring the promise of virtual reality in enhancing anatomy education: a focus group study with medical students
Authors: Abundez Toledo Maximiliano , Ghanem Ghadi , Fine Stuart , Weisman Daniel , Huang Yue Ming , Rouhani Amir A.
Published: March 2024

Virtual reality (VR) has the potential to be used as a transformative tool in medical education - offering both interactive models and clinical simulations to enhance training. VR presents a space and cost-effective solution for remote education, combined with prospects of higher efficiency and interactivity than traditional training. This study aimed to explore the potential application and perception of VR in a focus group of medical students as an innovative tool for learning anatomy. Sixteen students underwent a structured VR lesson plan exploring concepts in anatomy. Pre- and post-surveys assessed participants’ exposure to VR, previous exposure to and preparedness in anatomy training, and attitudes toward VR. Results revealed that despite limited prior exposure to VR, participants found the technology both easy to navigate and comfortable to use. Notably, over 90% of students indicated that VR would enhance their anatomy learning experience and help them learn a topic better than traditional models. Furthermore, 94% of participants agreed that this learning modality should be offered to medical students, and if given access to this technology, most would utilize it for learning anatomy and potentially for other subjects as well. This study emphasizes VR’s potential to enhance medical education, particularly in anatomy instruction. VR’s adaptability, user-friendly interface, and positive student perceptions highlight its viability as a supplemental tool. Future research should explore specific anatomy applications, long-term impacts on knowledge retention, and the evolving role of VR in medical education.
Recent progress of polymeric microneedle-assisted long-acting transdermal drug delivery
Authors: Meng Fanda , Qiao Xinyu , Xin Chenglong , Ju Xiaoli , He Meilin
Published: March 2024

Microneedle (MN)-assisted drug delivery technology has gained increasing attention over the past two decades. Its advantages of self-management and being minimally invasive could allow this technology to be an alternative to hypodermic needles. MNs can penetrate the stratum corneum and deliver active ingredients to the body through the dermal tissue in a controlled and sustained release. Long-acting polymeric MNs can reduce administration frequency to improve patient compliance and therapeutic outcomes, especially in the management of chronic diseases. In addition, long-acting MNs could avoid gastrointestinal reactions and reduce side effects, which has potential value for clinical application. In this paper, advances in design strategies and applications of long-acting polymeric MNs are reviewed. We also discuss the challenges in scale manufacture and regulations of polymeric MN systems. These two aspects will accelerate the effective clinical translation of MN products.
An Assessment of Food Value Chains to Identify Gaps and Make Recommendations for Further Development: A Slovenian Case Study
Authors: Prišenk Jernej , Turk Jernej , Pažek Karmen , Rozman Črtomir , Borec Andreja , Zidar Nejc
Published: March 2024

The content of this paper presents the research results of a three-year research project in which a multi-criteria evaluation model (according to the DEX methodology) was developed for the evaluation of three different food sectors (represented by a cattle breeding chain, a pig farming chain, and a milk production chain) with added value in Slovenia. Indicators for the assessment of the economically, socially, and environmentally sustainable development of food chains were taken into account. The data for the analysis, such as prices and costs of food, wage levels by sector, food miles and others, were obtained from various public services between 2020 and 2023. The final qualitative assessment of the food sectors was uniform (“average”), while the longest analysis of the results using the plus-minus-1 analysis method showed the reasons for such an assessment in individual sectors (such as the ratio between the price of agricultural products and the price of agricultural inputs is poor, the ratio between average gross salary in the individual food sector and gross salary in the agricultural sector is poor, etc.). In addition to the results already mentioned, recommendations or suggestions for building a sustainable food chain were made using the results of the modelling. The research results contributed to a better understanding of the importance of stable relationships between different groups of indicators and later showed their importance for improving the functioning of agri-food chains. The results of the research will help various stakeholders (such as the agricultural advisory service, decision-makers at the level of agricultural policy, researchers in further analyses, and especially the international professional public interested in various case studies from EU countries) to further analyse and plan for the organisation of the agricultural sector.
Economic Strategies and Policy Suggestions of Agricultural Sustainable Food Production
Authors: Altobelli Filiberto , Henke Roberto
Published: March 2024

Sustainability is increasingly becoming a keyword for viable agriculture and food production [...]
The Difference in Shoot Metabolite Profiles of a Wild and a Cultivated Barley Genotype in Response to Four Nitrogen Forms
Authors: Naz Shama , Khan Siffat Ullah , Kanwal Farah , Khan Ameer , Zhang Guoping
Published: March 2024

Plants can utilize different N forms, including organic and inorganic N resources, and show great differences in the utilization efficiency of each N form among species and genotypes within a species. Previously, we found that the Tibetan wild barley genotype (XZ16) was better in the utilization of organic nitrogen in comparison with the cultivated barley genotype (Hua30). In this study, the metabolite profiles of the two barley genotypes were comprehensively compared in their response to four N forms, including nitrate (NO3−), ammonium (NH4+), urea, and glycine. The macro and micro nutrient concentrations in shoots were mostly found to be higher in the nitrate and urea treatments than in ammonium and glycine in both the genotypes. XZ16 had higher concentrations of nutrient ions in the glycine treatment, but Hua30 accumulated more nutrients in the ammonium treatment. Among a total of 163 differentially regulated metabolites, the highest up-regulation and highest down-regulation values were found in XZ16 in the glycine and urea treatments, respectively. Some important metabolites, such as proline, glutamine, serine, asparagine, L-homoserine, aspartic acid, putrescine, ornithine, and 4-aminobutyrate, were up-regulated in the glycine treatment in both the genotypes with a higher fold change in XZ16 than that in Hua30. Similarly, fructose-6-PO4, aconitic acid, and isocitrate were only up-regulated in XZ16 in the glycine treatment. Here, we concluded that the genotype XZ16 exhibited a better response to the glycine treatment, while Hua30 showed a better response to the NH4+ treatment, which is attributed to the better utilization of glycine-N and NH4+-N, respectively.
Effects of Nano-Silica and Multi-Walled Carbon Nanotubes on Grape Seedlings under Salt Stress
Authors: Li Yang , Sheng Yinsheng , Shu Jing , Hao Shuqin , Wang Jinquan , Huang Qinglin , He Kailong , Qi Jiahui , Liu Jin
Published: March 2024

To improve the salt tolerance of grape seeds and seedlings under salt stress, this study was conducted including two control groups (CK, S) and five experimental groups (S + Si40, S + Si60, S + Si80, S + C90, S + Si40 + C90), and the physiological characteristics of grape seed germination and seedlings were studied using 40, 60, and 80 μg/mL of nano-silica treatments and by mixing 40 μg/mL of nano-silica with 90 μg/mL of multi-walled carbon nanotubes (MWCNTs), respectively. The combined treatment of 40 μg/mL nano-silica and 90 μg/mL MWCNTs resulted in the best rate of growth in grape seeds and root length and an increased germination rate when compared with the other concentrations. The combined treatment reduced the MDA content in the grape seedling leaves and increased the activities of superoxide (SOD), peroxidase (POD), catalase (CAT), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), ascorbate peroxidase (APX), glutathione-s-transferase (GT), and glutathione reductase (GR). In addition, the scavenging activity of DPPH· was also maintained by the combined treatment. In conclusion, a combined treatment with 40 μg/mL nano-silica and 90 μg/mL MWCNTs significantly increased the reduction capacity through the direct and indirect antioxidant systems (AsA-GSH cycle) and maintained a high antioxidant capacity of grape seedlings under salt stress.
First Report of Leaf Spot Disease (“Negrilla”) on Agave salmiana Otto Ex Salm-Dyck (ssp. salmiana) Plants Caused by Bipolaris zeae Zivan in Mexico
Authors: Romero-Cortes Teresa , Pérez España Victor H. , Pescador-Rojas José A. , Rangel-Cortés Eduardo , Armendaríz-Ontiveros María M. , Cuervo-Parra Jaime A.
Published: March 2024

Agave genera include slow-growing plants with cultural and economic roots dating back to pre-Columbian times in Mexico. Several species have a widespread presence in the country and are cultivated and/or used directly from the field to obtain various derived products. Agave salmiana is widely used in the region of the High Valleys of Apan, Hidalgo, Mexico. However, fungal diseases are causing considerable losses to Agave crops. For this reason, fungi strains from maguey plants from Apan, Hidalgo, with “Negrilla” disease symptoms were isolated and identified morphologically and molecularly. The results provide information on a new disease disseminated in A. salmiana plants, which causes symptoms such as black spots on the leaves due to pathogenic fungi of the genera Bipolaris. The morphological and molecular characterization located the phytopathogenic fungus as new isolates of Bipolaris zeae. Finally, the re-isolation of the causal agent of the disease was achieved in all pathogenicity tests, verifying that the symptoms observed in the maguey plants were caused by B. zeae, thus corroborating Koch’s postulates, and constituting the first report of this fungus as a pathogen of A. salmiana in Mexico.
Transcriptome Analysis of Potential Regulatory Genes under Chemical Doubling in Maize Haploids
Authors: Li Youqiang , Zhan Penglin , Pu Rumin , Xiang Wenqi , Meng Xin , Yang Shiqi , Hu Gaojiao , Zhao Shuang , Han Jialong , Xia Chao , Lan Hai , Wang Qingjun , Li Jingwei , Lu Yanli , Yu Yongtao , Liao Changjian , Li Gaoke , Lin Haijian
Published: March 2024

Maize is one of the most successful crops with regard to the utilization of heterosis. The haploid induction technique is one of the fastest methods to obtain pure maize material at the present stage. However, the molecular mechanism of haploid doubling is rarely reported. In this study, we treated B73 and ZNC442 haploid young shoots with colchicine for 0 h, 6.2 h, and 10 h, and analyzed the differentially expressed genes (DEGs). We found that colchicine treatment for 6.2 h and 10 h compared to 0 h resulted in a total of 4868 co-DEGs. GO enrichment analysis and KEGG metabolic pathway analysis found significantly enriched 282 GO terms and 31 significantly pathways, respectively. Additionally, The GO term and KEGG pathway genes of spindle, cytoskeleton, microtubules and nuclear division were selected for analysis, and three candidate genes were screened by taking intersections. Zm00001d033112, Zm00001d010525, and Zm00001d043386 were annotated as kinesin-associated protein 13, kinesin-like protein KIN-10C, and kinesin light-chain LC6, respectively. The real-time fluorescence quantification (RT-PCR) results revealed that Zm00001d033112, Zm00001d010525, and Zm00001d043386 had the same trends as RNA-seq. Interestingly, Zm00001d033112 is homologous gene AT3G20150 in Arabidopsis, which was involved in the regulation of chromosome movement and mitotic spindle assembly. Our study suggests that kinesin genes may play an important role in doubling chromosomes, thus providing valuable information for future studies on the molecular mechanisms of chromosome doubling in maize.