bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterizing Pathways of Non-oral Prescription Stimulant Non-medical Use Among Adults Recruited From Reddit
Authors: Vosburg Suzanne K. , Robbins Rebekkah S. , Antshel Kevin M. , Faraone Stephen V. , Green Jody L.
Published: January 2021

Objective: Prescription stimulant non-medical use (NMU) is a national predicament. While the risks of prescription stimulant NMU have been considered, less is known about non-oral use. To focus on this gap, a sample of adults with non-oral prescription stimulant NMU within the last 5-years was recruited. The purpose of the present study was to characterize the pathways and substance transitions associated with prescription stimulant NMU and non-oral prescription stimulant NMU in this unique sample of adults.

Methods: Adults (n = 225) reporting non-oral prescription stimulant NMU within the last 5 years were recruited to complete an online survey by banner ads placed on the Reddit website between February and September 2019. After completion of the survey, a second study consisting of an in-depth telephone interview was conducted with 23 participants: interviews took place between July and September 2019. Data reported here include substance, route of administration and class transitions, as well as qualitative data from the interviews.

Results: Approximately 1 in 5 began their substance use trajectory with prescription stimulants (19.1%). Other than marijuana, most exposures to illicit substances occurred after both initial prescription stimulant NMU and initial non-oral prescription stimulant NMU. The most frequently reported route of administration transition was from oral use to snorting (n = 158, 70.2%), however, other route of administration transitions included oral use to injection drug use (n = 14, 6%). In-depth interviews elaborated upon these transitions and indicated that prescription stimulant NMU was consequential to substance use pathways.

Conclusions: Oral prescription stimulant NMU was a precursor to non-oral prescription stimulant NMU. Non-oral prescription stimulant NMU was a precursor to illicit substance use, suggesting that prescription stimulant NMU impacts substance use pathways and revealing opportunities for intervention.
COVID-19 and Teleneurology in Sub-Saharan Africa: Leveraging the Current Exigency
Authors: Adebayo Philip Babatunde , Oluwole Olusegun John , Taiwo Funmilola Tolulope
Published: January 2021

Africa has over 1.3 billion inhabitants, with over 60% of this population residing in rural areas that have poor access to medical experts. Despite having a ridiculously huge, underserved population, very few African countries currently have any form of sustained and organized telemedicine practice, and even fewer have dedicated tele-neurology services. The ongoing COVID-19 pandemic has proved to be one of the most significant disruptors of vital sectors of human endeavor in modern times. In the healthcare sector, there is an increasing advocacy to deliver non-urgent care via telemedicine. This paper examined the current state of tele-neurology practice and infrastructural preparedness in sub-Saharan Africa. Currently, there is over 70% mobile phone penetration in most of the countries and virtually all of them have mobile internet services of different technologies and generations. Although the needed infrastructure is increasingly available, it should be improved upon. We have proposed the access, costs, ethics, and support (ACES) model as a bespoke, holistic strategy for the successful implementation and advancement of tele-neurology in sub-Saharan Africa.
Identifying Ingredient Substitutions Using a Knowledge Graph of Food
Authors: Shirai Sola S. , Seneviratne Oshani , Gordon Minor E. , Chen Ching-Hua , McGuinness Deborah L.
Published: January 2021

People can affect change in their eating patterns by substituting ingredients in recipes. Such substitutions may be motivated by specific goals, like modifying the intake of a specific nutrient or avoiding a particular category of ingredients. Determining how to modify a recipe can be difficult because people need to 1) identify which ingredients can act as valid replacements for the original and 2) figure out whether the substitution is “good” for their particular context, which may consider factors such as allergies, nutritional contents of individual ingredients, and other dietary restrictions. We propose an approach to leverage both explicit semantic information about ingredients, encapsulated in a knowledge graph of food, and implicit semantics, captured through word embeddings, to develop a substitutability heuristic to rank plausible substitute options automatically. Our proposed system also helps determine which ingredient substitution options are “healthy” using nutritional information and food classification constraints. We evaluate our substitutability heuristic, diet-improvement ingredient substitutability heuristic (DIISH), using a dataset of ground-truth substitutions scraped from ingredient substitution guides and user reviews of recipes, demonstrating that our approach can help reduce the human effort required to make recipes more suitable for specific dietary needs.
Major Molecular Signaling Pathways in Oral Cancer Associated With Therapeutic Resistance
Authors: Usman Saima , Jamal Ahmad , Teh Muy-Teck , Waseem Ahmad
Published: January 2021

Oral cancer is a sub-category of head and neck cancers that primarily initiates in the oral cavity. The primary treatment option for oral cancer remains surgery but it is associated with massive disfigurement, inability to carry out normal oral functions, psycho-social stress and exhaustive rehabilitation. Other treatment options such as chemotherapy and radiotherapy have their own limitations in terms of toxicity, intolerance and therapeutic resistance. Immunological treatments to enhance the body's ability to recognize cancer tissue as a foreign entity are also being used but they are new and underdeveloped. Although substantial progress has been made in the treatment of oral cancer, its complex heterogeneous nature still needs to be explored, to elucidate the molecular basis for developing resistance to therapeutic agents and how to overcome it, with the aim of improving the chances of patients' survival and their quality of life. This review provides an overview of up-to-date information on the complex role of the major molecules and associated signaling, epigenetic changes, DNA damage repair systems, cancer stem cells and micro RNAs in the development of therapeutic resistance and treatment failure in oral cancer. We have also summarized the current strategies being developed to overcome these therapeutic challenges. This review will help not only researchers but also oral oncologists in the management of the disease and in developing new therapeutic modalities.
Methodology for the Field Evaluation of the Impact of Augmented Reality Tools for Maintenance Workers in the Aeronautic Industry
Authors: Loizeau Quentin , Danglade Florence , Ababsa Fakhreddine , Merienne Frédéric
Published: January 2021

Augmented Reality (AR) enhances the comprehension of complex situations by making the handling of contextual information easier. Maintenance activities in aeronautics consist of complex tasks carried out on various high-technology products under severe constraints from the sector and work environment. AR tools appear to be a potential solution to improve interactions between workers and technical data to increase the productivity and the quality of aeronautical maintenance activities. However, assessments of the actual impact of AR on industrial processes are limited due to a lack of methods and tools to assist in the integration and evaluation of AR tools in the field. This paper presents a method for deploying AR tools adapted to maintenance workers and for selecting relevant evaluation criteria of the impact in an industrial context. This method is applied to design an AR tool for the maintenance workshop, to experiment on real use cases, and to observe the impact of AR on productivity and user satisfaction for all worker profiles. Further work aims to generalize the results to the whole maintenance process in the aeronautical industry. The use of the collected data should enable the prediction of the impact of AR for related maintenance activities.
Why Multi-Family Groups for Transgender Adolescents and Their Parents?
Authors: Rabain Nicolas S.
Published: January 2021

The author presents a therapeutic approach for contemporary families carried out in an innovative mental health care setting. This approach involves receiving both transgender adolescents and their parents. Initially, the adolescents are brought together in a discussion group. Then, the parents of these adolescents are gathered without their children in order to reflect on family relations and social adaptation and to receive support when needed. Both groups are gathered together a couple of times a year in a multi-family meeting. Using a dynamic based on the principle of free association and the interplay of identifications among participants, the author points out how these groups and multi-family gatherings promote mental health and prevent mental disorders and the disruption of family relations.
Mergers of Binary Neutron Star Systems: A Multimessenger Revolution
Authors: Pian Elena
Published: January 2021

On August 17, 2017, less than two years after the direct detection of gravitational radiation from the merger of two∼30 M⊙ black holes, a binary neutron star merger was identified as the source of a gravitational wave signal of ∼100 s duration that occurred at less than 50 Mpc from Earth. A short gamma-ray burst was independently identified in the same sky area by the Fermi and INTEGRAL satellites for high energy astrophysics, which turned out to be associated with the gravitational event. Prompt follow-up observations at all wavelengths led first to the detection of an optical and infrared source located in the spheroidal Galaxy NGC4993 and, with a delay of ∼10 days, to the detection of radio and X-ray signals. This article revisits these observations and focusses on the early optical/infrared source, which was thermal in nature and powered by the radioactive decay of the unstable isotopes of elements synthesized via rapid neutron capture during the merger and in the phases immediately following it. The far-reaching consequences of this event for cosmic nucleosynthesis and for the history of heavy elements formation in the Universe are also illustrated.
Peste des Petits Ruminants Risk Factors and Space-Time Clusters in Bangladesh
Authors: Rahman A. K. M. Anisur , Islam Sk Shaheenur , Sufian Md. Abu , Talukder Md. Hasanuzzaman , Ward Michael P. , Martínez-López Beatriz
Published: January 2021

Peste des Petits Ruminants (PPR) is endemic in Bangladesh, but its spatial distribution and risk factors have not yet been reported. Using four years of national-level, passive surveillance data (2014 to 2017), in this study we aimed to identify risk factors, create PPR risk maps and describe PPR time-space clusters. We selected PPR case records—mainly based on presumptive diagnosis of small ruminants in subdistrict veterinary hospitals—and sheep and goat population data from all 64 districts of Bangladesh. Peste des Petits Ruminants cumulative incidence per 10,000 animals at risk per district was used to conduct cluster and hotspot analysis and create predictive maps for each year and all 4 years combined. The association between PPR cumulative incidence and hypothesized risk factors—including climatic variables, elevation, road length, river length, railroad length, land cover, and water bodies—was analyzed using a geographically weighted regression model. The total number of PPR cases reported during the study period was 5.2 million. We found that most PPR cases (27.6%) were reported in the monsoon season. The highest and lowest proportions of cases were reported from Rajshahi (36.1%) and Barisal divisions (2.1%), respectively. We identified five space-time clusters, 9 high–high clusters, and 9 hotspots. The predicted cumulative incidences of PPR were persistently higher in north-east, north-west, and south-east parts of Bangladesh. Road length (P = 0.03) was positively associated with PPR incidence in Bangladesh. Results suggest that movement of animals (road length) plays an important role in the epidemiology of PPR in Bangladesh. Along with restriction of animal movement, hotspots and high–high clusters should be targeted first for immunization coverage in Bangladesh and similar PPR endemic countries to achieve eradication.
Analysis of the Nonlinear Response of Piezo-Micromirrors with the Harmonic Balance Method
Authors: Opreni Andrea , Boni Nicolò , Carminati Roberto , Frangi Attilio
Published: January 2021

In this work, we address the simulation and testing of MEMS micromirrors with hardening and softening behaviour excited with patches of piezoelectric materials. The forces exerted by the piezoelectric patches are modelled by means of the theory of ferroelectrics developed by Landau–Devonshire and are based on the experimentally measured polarisation hysteresis loops. The large rotations experienced by the mirrors also induce geometrical nonlinearities in the formulation up to cubic order. The solution of the proposed model is performed by discretising the device geometry using the Finite Element Method, and the resulting large system of coupled differential equations is solved by means of the Harmonic Balance Method. Numerical results were validated with experimental data collected on the devices.
Connection between Nutrient Content and Resistance to Selected Pests Analyzed in Brassicaceae Hybrids
Authors: Szwarc Justyna , Niemann Janetta , Bocianowski Jan , Jakubus Monika , Mrówczyński Marek
Published: January 2021

Rapeseed (Brassica napus L. ssp. oleifera Metzg) is attacked by a wide range of pests. Breeding of resistant cultivars is one of the best methods of protecting crops against insects, and non-cultivated species of Brassicaceae can be used as resistance donors. In this study, we investigated the relationship between nutrient content and resistance to two commonly occurring pests (Delia radicum and Brevicoryne brassicae) in 12 Brassicaceae hybrid combinations. The experiment was conducted at Poznań University of Life Sciences Experimental Station located in Dłoń. The assessment was carried out for three years (2017, 2018, and 2019) on randomly chosen individuals in two growth stages: vegetative stage and flowering stage. The resistance to B. brassicae and D. radicum was observed in all three years, however, macronutrient and micronutrient contents as well as total nitrogen, total sulfur, and sulfur sulfate were observed only in 2019. Statistically significant negative association between Ca and resistance to B. brassicae and D. radicum was discovered. Additionally, positive association between Cu and resistance to B. brassicae was observed. The importance of selected mineral elements in plant resistance is widely discussed.
Potassium and Nitrogen Fertilization vs. Trace Element Content of Maize (Zea mays L.)
Authors: Wyszkowski Mirosław , Brodowska Marzena S.
Published: January 2021

This research was conducted to study the impact of potassium and nitrogen fertilizers on the trace element content of maize (Zea mays L.). Higher doses of potassium fertilizer led to a linear increase in cadmium, lead, nickel, zinc, and manganese content, and to lower Fe:Zn and Fe:Mn ratios. Moreover, cobalt level increased in maize upon the lower doses and decreased upon the higher doses of potassium. The impact of potassium fertilizer on the levels of other elements (iron and chromium) in maize was determined by the fertilizer dose and, in particular, by the supply of additional nitrogen. Potassium fertilization led to a higher bioconcentration (BCFactor) of most of the trace elements in the aerial parts of maize. Nitrogen fertilization led to increased contents of manganese and iron, increased Fe:Zn and Fe:Mn ratios, and decreased contents of cadmium, lead, nickel, and cobalt in maize. Compared with potassium, nitrogen fertilization produced less consistent changes in bioconcentration factors for trace elements in the aerial parts of maize.
Soybean Development and Productivity in Response to Organic Management above the Northern Boundary of Soybean Distribution in Europe
Authors: Toleikiene Monika , Slepetys Jonas , Sarunaite Lina , Lazauskas Sigitas , Deveikyte Irena , Kadziuliene Zydre
Published: January 2021

Climate change, new varieties, better technological abilities, and increased demand for local resources provide significant reasons to introduce soybeans in northern regions, above the typical soybean distribution area in Europe. This research examined the effects of two delayed sowing times, wide 25 cm and 50 cm row spacings, seed inoculation with Bradyrhizobium japonicum, and the interaction of all these factors on soybean development and productivity in an organic farming system. Length of soybean vegetation varied from 142 to 161 days at latitude 55° N. Yield varied from 673 to 3154 kg ha−1 in response to management factors. In the dry 2015 year, the combination of later sowing dates and wide 50-cm row spacing significantly (p < 0.01) increased the number of pods per plant by 28%, aboveground dry biomass by two times, and seed yield by 36% plant−1. In the wet 2016, yield components reached their highest values of 16.8 g dry biomass, 19.9 pods plant−1 and 7.9 g seeds plant−1 when inoculated soybeans were sown earlier, with 50-cm row spacings. Protein content significantly varied from 27.4 to 35.3%, and fat content 17.4–21.5%. This study suggests that regular soybean development could be maintained in organically managed locations above the present northern soybean distributional region, but its development, productivity, and production quality significantly depends on management practices.
Comparative Behavior of Dracaena marginata Plants Integrated into a Cascade Cropping System with the Addition of Hydrogen Peroxide
Authors: García-Caparrós Pedro , Espino Cristina Velasquez , Lao María Teresa
Published: January 2021

The reuse of crop drainage into other crops, in the form of a cascade cropping system, is a feasible environmental solution where high inputs of water and fertilizer are used for crop growth and lower efficiency rates, associated with a high discharge of water and fertilizers into the environment, are present. Dracaena marginata plants were cultured in containers with sphagnum peat moss and were subjected to three different fertigation treatments for eight weeks: Dm0 (standard nutrient solution or control treatment), Dm1 (raw leachates), and Dm2 (raw leachates with additional H2O2), where the leachates were collected from a Chrysalidocarpus lutescens-Dracaena deremensis cascade cropping system. At the end of the harvesting, growth parameters, pigment concentration, leaf and root proline, total soluble sugar concentrations, and water and nutrient use efficiencies were assessed for each fertigation treatment. Plant height, root, stem, and total dry weight increased under fertigation with leachates with H2O2. The fertigation with leachates with or without H2O2 increased the red index value. There were no clear trends between the fertigation treatments with regards to pigment concentrations and biochemical parameters (proline and total soluble sugar concentrations). The addition of H2O2 to the leachate increased N concentration in the organs assessed, as well as the water and nutrient use efficiencies. There were no variations in H2PO4−, SO42−, Na+, and Mg2+ concentration in the chemical composition of the substrate between fertigation treatments. The positive results reported in this experiment suggest the potential growth of Dracaena marginata with leachate and hydrogen peroxide in a cascade cropping system.
Dairy Caretaker Perspectives on Performing Euthanasia as an Essential Component of Their Job
Authors: Román-Muñiz Ivette Noami , Cramer Mary Caitlin , Edwards-Callaway Lily N. , Stallones Lorann , Kim Elizabeth , Thompson Sofia , Simpson Hailey , Mijares Sage
Published: January 2021

The objectives of this study were to identify caretakers’ perceptions and stressors related to performing on-farm euthanasia as well as potential ideas for intervention strategies to alleviate negative effects of performing euthanasia on caretaker well-being. Additionally, we aimed to determine how euthanasia methods, procedures, and training correlate with dairy caretakers’ attitudes toward performing on-farm euthanasia and their job satisfaction and sense of well-being. Thirty-eight dairy caretakers (19 workers, 15 supervisors, 3 owners, 1 veterinarian) participated in focus groups or interviews conducted and recorded on five Northern Colorado dairies. Thematic analysis of focus group and interview transcripts revealed seven recurring themes. Variation in available training and euthanasia protocols among dairy farms was evident. There was a lack of awareness regarding available mental health resources and little communication between farm personnel about euthanasia-related stress. Training was correlated with caretaker knowledge about euthanasia procedures and the language used to refer to euthanasia. Human-animal bonds and empathy toward animals were evident in participants regardless of training, job position, or dairy experience. Interventions such as training programs, mental health resources, and other support systems should be tested for effectiveness in addressing euthanasia-related stressors, improving euthanasia decision-making and practice, and increasing animal wellbeing on dairy farms.
Long Noncoding RNA LncPGCR Mediated by TCF7L2 Regulates Primordial Germ Cell Formation in Chickens
Authors: Jiang Jingyi , Chen Chen , Cheng Shaoze , Yuan Xia , Jin Jing , Zhang Chen , Sun Xiaolin , Song Jiuzhou , Zuo Qisheng , Zhang Yani , Chen Guohong , Li Bichun
Published: January 2021

Although lncRNAs have been identified as playing critical roles in the development of germ cells, their potential involvement in the development of PGCs in chickens remains poorly understood. Differentially expressed lncRNAs (DELs) from previous RNA-seq of embryonic stem cells (ESCs), PGCs, and spermatogonial stem cells (SSCs) were analyzed by K-means clustering, from which a key candidate, lncRNA (lncRNA PGC regulator, LncPGCR) was obtained. We confirmed that LncPGCR plays a positive role in the development of PGCs by increasing the expression of the PGC marker gene (Cvh and C-kit), while downregulating the pluripotency-associated gene (Nanog) in vitro and in vivo. The activation and expression of LncPGCR are regulated by histone acetylation, and transcription factor TCF7L2. Mechanistically, a rescue assay was performed to further confirm that LncPGCR contributed to the development of PGCs by regulating the gga-miR-6577-5p/Btrc signaling pathway. Adsorption of gga-miR-6577-5p activated the WNT signaling cascade by relieving the gga-miR-6577-5p-dependent inhibition of Btrc expression. Taken together, our study discovered the growth-expedited role of LncPGCR in PGCs development, showing the potential LncPGCR/miR-6577-5p/Btrc pathway. The results and findings provide a novel insight into the development of PGCs.
Temperament, Plasticity, and Emotions in Defensive Behaviour of Paca (Mammalia, Hystricognatha)
Authors: Nogueira Selene S. C. , Nogueira-Filho Sérgio L. G. , Duarte José M. B. , Mendl Michael
Published: January 2021

Within a species, some individuals are better able to cope with threatening environments than others. Paca (Cuniculus paca) appear resilient to over-hunting by humans, which may be related to the behavioural plasticity shown by this species. To investigate this, we submitted captive pacas to temperament tests designed to assess individual responses to short challenges and judgement bias tests (JBT) to evaluate individuals’ affective states. Results indicated across-time and context stability in closely correlated “agitated”, “fearful” and “tense” responses; this temperament dimension was labelled “restless”. Individual “restless” scores predicted responses to novelty, although not to simulated chasing and capture by humans in a separate modified defence test battery (MDTB). Restless animals were more likely to show a greater proportion of positive responses to an ambiguous cue during JBT after the MDTB. Plasticity in defensive behaviour was inferred from changes in behavioural responses and apparently rapid adaptation to challenge in the different phases of the MDTB. The results indicate that both temperament and behavioural plasticity may play a role in influencing paca responses to risky situations. Therefore, our study highlights the importance of understanding the role of individual temperament traits and behavioural plasticity in order to better interpret the animals’ conservation status and vulnerabilities.
Proteomic Analysis of the Secretome and Exosomes of Feline Adipose-Derived Mesenchymal Stem Cells
Authors: Villatoro Antonio J. , Martín-Astorga María del Carmen , Alcoholado Cristina , Sánchez-Martín María del Mar , Becerra José
Published: January 2021

Mesenchymal stem cells (MSCs) have been shown to have therapeutic efficacy in different complex pathologies in feline species. This effect is attributed to the secretion of a wide variety of bioactive molecules and extracellular vesicles, such as exosomes, with significant paracrine activity, encompassed under the concept of the secretome. However, at present, the exosomes from feline MSCs have not yet been studied in detail. The objective of this study is to analyze and compare the protein profiles of the secretome as a whole and its exosomal fraction from feline adipose-derived MSCs (fAd-MSCs). For this, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Protein–Protein Interaction Networks Functional Enrichment Analysis (STRING) were utilized. A total of 239 proteins were identified in the secretome, and 228 proteins specific to exosomes were identified, with a total of 133 common proteins. The proteins identified in the secretome were located in the extracellular regions and in the cytoplasm, while the exosomal proteins were located mainly in the membrane, cytoplasm and cytosol. Regarding function, in the secretome, proteins involved in different metabolic pathways, in pathways related to the immune system and the endocrine system and in the processing of proteins in the endoplasmic reticulum predominated. In contrast, proteins specific to exosomes were predominantly associated with endocytosis, cell junctions, platelet activation and other cell signaling pathways. The possible future use of the secretome, or some of its components, such as exosomes, would provide a non-cell-based therapeutic strategy for the treatment of different diseases that would avoid the drawbacks of cell therapy.
Chronic Wasting Disease (CWD) in Sami Reindeer Herding: The Socio-Political Dimension of an Epizootic in an Indigenous Context
Authors: Maraud Simon , Roturier Samuel
Published: January 2021

Chronic wasting disease (CWD) is the most transmissible of the prion diseases. In 2016, an unexpected case was found in Norway, the first in Europe. Since then, there have been 32 confirmed cases in Norway, Sweden, and Finland. This paper aims to examine the situation from a social and political perspective: considering the management of CWD in the Swedish part of Sápmi—the Sami ancestral land; identifying the place of the Sami people in the risk management–because of the threats to Sami reindeer herding that CWD presents; and understanding how the disease can modify the modalities of Indigenous reindeer husbandry, whether or not CWD is epizootic. Based on interviews with various stakeholders and by examining the social sciences literature, this paper shows that the health risk management is structured by a politico-scientific controversy about the recognition, or not, of atypical and classical CWD. The Sami herders are currently cooperating with the state authorities in the surveillance program to sample their herds. This involvement takes place in a situation where the balance of power between the Sami people and the state, or the European Union, is framed by its colonial context. This has consequences with respect to the definition of a common interest and to implementing sanitary measures. The particular features of reindeer herding are seen as a challenge to managing CWD risk, compared with European health standards. We argue that CWD will greatly modify the modalities of Indigenous reindeer herding, whether there are positive cases or not in the Sami reindeer. By implementing new health guidelines, the authorities will create a cascading effect in Sami land and its use. The CWD situation in Fennoscandia is full of uncertainty but may cause a major shift in the organization and the governance of Sápmi. In September 2020, the identification of a new CWD case in a wild reindeer in Norway started a new episode in the disease management in Fennoscandia. Our paper raises various questions linked to understanding this new step in this crisis which is not only epidemiological, but also socio-cultural and political.
Rhodomyrtone as a New Natural Antibiotic Isolated from Rhodomyrtus tomentosa Leaf Extract: A Clinical Application in the Management of Acne Vulgaris
Authors: Wunnoo Suttiwan , Bilhman Siwaporn , Amnuaikit Thanaporn , Ontong Julalak C. , Singh Sudarshan , Auepemkiate Sauvarat , Voravuthikunchai Supayang P.
Published: January 2021

Rhodomyrtone, a plant-derived principal compound isolated from Rhodomyrtus tomentosa (Myrtaceae) leaf extract, was assessed as a potential natural alternative for the treatment of acne vulgaris. The clinical efficacy of a 1% liposomal encapsulated rhodomyrtone serum was compared with a marketed 1% clindamycin gel. In a randomized and double-blind controlled clinical trial, 60 volunteers with mild to moderate acne severity were assigned to two groups: rhodomyrtone serum and clindamycin gel. The volunteers were instructed to apply the samples to acne lesions on their faces twice daily. A significant reduction in the total numbers of acne lesions was demonstrated in both treatment groups between week 2 and 8 (p < 0.05). Significant differences in acne numbers compared with the baseline were evidenced at week 2 onwards (p < 0.05). At the end of the clinical trial, the total inflamed acne counts in the 1% rhodomyrtone serum group were significantly reduced by 36.36%, comparable to 34.70% in the clindamycin-treated group (p < 0.05). Furthermore, a commercial prototype was developed, and a clinical assessment of 45 volunteers was performed. After application of the commercial prototype for 1 week, 68.89% and 28.89% of volunteers demonstrated complete and improved inflammatory acne, respectively. All of the subjects presented no signs of irritation or side effects during the treatment. Most of the volunteers (71.11%) indicated that they were very satisfied. Rhodomyrtone serum was demonstrated to be effective and safe for the treatment of inflammatory acne lesions.
Elicitation of Stress-Induced Phenolic Metabolites for Antimicrobial Applications against Foodborne Human Bacterial Pathogens
Authors: Christopher Ashish , Sarkar Dipayan , Shetty Kalidas
Published: January 2021

Foodborne bacterial pathogens in consumed foods are major food safety concerns worldwide, leading to serious illness and even death. An exciting strategy is to use novel phenolic compounds against bacterial pathogens based on recruiting the inducible metabolic responses of plant endogenous protective defense against biotic and abiotic stresses. Such stress-inducible phenolic metabolites have high potential to reduce bacterial contamination, and particularly improve safety of plant foods. The stimulation of plant protective response by inducing biosynthesis of stress-inducible phenolics with antimicrobial properties is among the safe and effective strategies that can be targeted for plant food safety and human gut health benefits. Metabolically driven elicitation with physical, chemical, and microbial elicitors has shown significant improvement in the biosynthesis of phenolic metabolites with antimicrobial properties in food and medicinal plants. Using the above rationale, this review focuses on current advances and relevance of metabolically driven elicitation strategies to enhance antimicrobial phenolics in plant food models for bacterial-linked food safety applications. Additionally, the specific objective of this review is to explore the potential role of redox-linked pentose phosphate pathway (PPP) regulation for enhancing biosynthesis of stress-inducible antibacterial phenolics in elicited plants, which are relevant for wider food safety and human health benefits.
Effects of Essential Oils from Cymbopogon spp. and Cinnamomum verum on Biofilm and Virulence Properties of Escherichia coli O157:H7
Authors: Scotti Raffaella , Stringaro Annarita , Nicolini Laura , Zanellato Miriam , Boccia Priscilla , Maggi Filippo , Gabbianelli Roberta
Published: January 2021

Every year, the pharmaceutical and food industries produce over 1000 tons of essential oils (EOs) exploitable in different fields as the development of eco-friendly and safe antimicrobial inhibitors. In this work we investigated the potential of some EOs, namely Cinnamomum verum, Cymbopogon martini, Cymbopogoncitratus and Cymbopogon flexuosus, on the growth, biofilm formation and gene expression in four strains of enterohemorrhagic Escherichia coli O157:H7. All EOs were analyzed by gas chromatography-mass spectrometry (GC-MS). The antimicrobial activity was performed by using dilutions of EOs ranging from 0.001 to 1.2% (v/v). Subinhibitory doses were used for biofilm inhibition assay. The expression profiles were obtained by RT-PCR. E. coli O157:H7 virulence was evaluated in vivo in the nematode Caenorhabditis elegans. All EOs showed minimal inhibitory concentrations (MICs) ranging from 0.0075 to 0.3% (v/v). Cinnamomum verum bark EO had the best activity (MIC of 0.0075% (v/v) in all strains) while the C. verum leaf EO had an intermediate efficacy with MIC of 0.175% (v/v) in almost all strains. The Cymbopogon spp. showed the more variable MICs (ranging from 0.075 to 0.3% (v/v)) depending on the strain used. Transcriptional analysis showed that C. martini EO repressed several genes involved in biofilm formation, virulence, zinc homeostasis and encoding some membrane proteins. All EOs affected zinc homeostasis, reducing ykgM and zinT expression, and reduced the ability of E. coli O157:H7 to infect the nematode C. elegans. In conclusion, we demonstrated that these EOs, affecting E. coli O157:H7 infectivity, have a great potential to be used against infections caused by microorganisms.
Brewing By-Product Upcycling Potential: Nutritionally Valuable Compounds and Antioxidant Activity Evaluation
Authors: Bravi Elisabetta , Francesco Giovanni De , Sileoni Valeria , Perretti Giuseppe , Galgano Fernanda , Marconi Ombretta
Published: January 2021

The brewing industry produces high quantities of solid and liquid waste, causing disposal issues. Brewing spent grains (BSGs) and brewing spent hop (BSH) are important by-products of the brewing industry and possess a high-value chemical composition. In this study, BSG and BSH, obtained from the production process of two different types of ale beer (Imperial red and Belgian strong beer) were characterized in terms of valuable components, including proteins, carbohydrates, fat, dietary fiber, β-glucans, arabinoxylans, polyphenols, and phenolic acids, and antioxidant activity (Ferric Reducing Antioxidant Power Assay (FRAP), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)). Significant concentrations of total polyphenols were observed in both BSH and BSG samples (average of about 10 mg GAE/g of dry mass); however, about 1.5-fold higher levels were detected in by-products of Belgian strong ale beer compared with Imperial red. Free and bound phenolic acids were quantified using a validated chromatographic method. A much higher level of total phenolic acids (TPA) (about 16-fold higher) was found in BSG samples compared with BSHs. Finally, their antioxidant potential was verified. By-products of Belgian strong ale beer, both BSG and BSH, showed significantly higher antioxidative capacity (about 1.5-fold lower inhibitory concentration (IC50) values) compared with spent grains and hop from the brewing of Imperial red ale. In summary, BSG and BSH may be considered rich sources of protein, carbohydrates, fiber, and antioxidant compounds (polyphenols), and have the potential to be upcycled by transformation into value-added products.
Automated Accuracy Assessment of a Mobile Mapping System with Lightweight Laser Scanning and MEMS Sensors
Authors: Al-Durgham Kaleel , Lichti Derek D. , Kwak Eunju , Dixon Ryan
Published: January 2021

The accuracy assessment of mobile mapping system (MMS) outputs is usually reliant on manual labor to inspect the quality of a vast amount of collected geospatial data. This paper presents an automated framework for the accuracy assessment and quality inspection of point cloud data collected by MMSs operating with lightweight laser scanners and consumer-grade microelectromechanical systems (MEMS) sensors. A new, large-scale test facility has been established in a challenging navigation environment (downtown area) to support the analyses conducted in this research work. MMS point cloud data are divided into short time slices for comparison with the higher-accuracy, terrestrial laser scanner (TLS) point cloud of the test facility. MMS data quality is quantified by the results of registering the point cloud of each slice with the TLS datasets. Experiments on multiple land vehicle MMS point cloud datasets using a lightweight laser scanner and three different MEMS devices are presented to demonstrate the effectiveness of the proposed method. The mean accuracy of a consumer grade MEMS (<$100) was found to be 1.13 ± 0.47 m. The mean accuracy of two commercial MEMS (>$100) was in the range of 0.48 ± 0.23 m to 0.85 ± 0.52 m. The method presented here in can be straightforwardly implemented and adopted for the accuracy assessment of other MMSs types such as unmanned aerial vehicles (UAV)s.
Virtual Surgical Planning and the “In-House” Rapid Prototyping Technique in Maxillofacial Surgery: The Current Situation and Future Perspectives
Authors: Maglitto Fabio , Dell’Aversana Orabona Giovanni , Committeri Umberto , Salzano Giovanni , De Fazio Gianluca Renato , Vaira Luigi Angelo , Abbate Vincenzo , Bonavolontà Paola , Piombino Pasquale , Califano Luigi
Published: January 2021

Background: The first applications of computer-aided design/computer-aided manufacturing (CAD/CAM) in maxillofacial surgery date back to the 1980s. Since then, virtual surgical planning (VSP) has undergone significant development and is now routinely used in daily practice. Indeed, in an extraordinary period, such as that of the current COVID-19 pandemic, it offers a valuable tool in relation to the protection of healthcare workers. In this paper we provide a comprehensive summary of the clinical applications reported in the literature and review our experience using an in-house rapid prototyping technique in the field of maxillofacial surgery. methods: Our research was focused on reconstructive surgery, traumatology (especially in relation to orbital floor and zygomatic arch fractures), and COVID-19 masks. The first step was a radiographic study. Next, computed tomography (CT) scans were segmented in order to obtain a three-dimensional (3D) model. Finally, in the editing phase, through the use of specific software, a customized device for each patient was designed and printed. results: Four reconstructive procedures were performed with a perfect fitting of the surgical device produced by means of VSP. In nine orbital floor fracture cases a good overlapping of the mesh on the orbital floor was obtained. In sixteen zygomatic arch cases the post-operative CT scan showed an excellent fitting of the device and a correct fracture reduction. Regarding the COVID-19 period, six masks and shields produced proved to provide effective protection. conclusions: The timescale and costs required for the production of our “home-made” virtual design are low, which makes this method applicable to a large number of cases, for both ordinary and extraordinary activities.
Towards Blockchain-Based Federated Machine Learning: Smart Contract for Model Inference
Authors: Drungilas Vaidotas , Vaičiukynas Evaldas , Jurgelaitis Mantas , Butkienė Rita , Čeponienė Lina
Published: January 2021

Federated learning is a branch of machine learning where a shared model is created in a decentralized and privacy-preserving fashion, but existing approaches using blockchain are limited by tailored models. We consider the possibility to extend a set of supported models by introducing the oracle service and exploring the usability of blockchain-based architecture. The investigated architecture combines an oracle service with a Hyperledger Fabric chaincode. We compared two logistic regression implementations in Go language—a pure chaincode and an oracle service—at various data (2–32 k instances) and network (3–13 peers) sizes. Experiments were run to assess the performance of blockchain-based model inference using 2D synthetic and EEG eye state datasets for a supervised machine learning detection task. The benchmarking results showed that the impact on performance is acceptable with the median overhead of oracle service reaching 2–4%, depending on the dimensionality of the dataset. The overhead tends to diminish at large dataset sizes with the runtime depending on the network size linearly, where additional peers increased the runtime by 6.3 and 6.6 s for 2D and EEG datasets, respectively. Demonstrated negligible difference between implementations justifies the flexible choice of model in the blockchain-based federated learning and other machine learning applications.
Generalization-Based Acquisition of Training Data for Motor Primitive Learning by Neural Networks
Authors: Lončarević Zvezdan , Pahič Rok , Ude Aleš , Gams Andrej
Published: January 2021

Autonomous robot learning in unstructured environments often faces the problem that the dimensionality of the search space is too large for practical applications. Dimensionality reduction techniques have been developed to address this problem and describe motor skills in low-dimensional latent spaces. Most of these techniques require the availability of a sufficiently large database of example task executions to compute the latent space. However, the generation of many example task executions on a real robot is tedious, and prone to errors and equipment failures. The main result of this paper is a new approach for efficient database gathering by performing a small number of task executions with a real robot and applying statistical generalization, e.g., Gaussian process regression, to generate more data. We have shown in our experiments that the data generated this way can be used for dimensionality reduction with autoencoder neural networks. The resulting latent spaces can be exploited to implement robot learning more efficiently. The proposed approach has been evaluated on the problem of robotic throwing at a target. Simulation and real-world results with a humanoid robot TALOS are provided. They confirm the effectiveness of generalization-based database acquisition and the efficiency of learning in a low-dimensional latent space.
Study of Materials Behavior in a Monumental Vault Strengthened by a Carbon Net in a Mineral Matrix Subjected to Seismic Influence
Authors: Bednarz Łukasz , Drygała Izabela , Dulińska Joanna , Jasieńko Jerzy
Published: January 2021

The application of the elasto-plastic material model known as the Barcelona Model (BM) for numerical assessment of a historical vault subjected to earthquake sequence is presented in this work. As a case study, part of a masonry vault erected in Southern Poland in the 12th century was chosen. For the study purposes, a 3D finite element model (FEM) of the vault was prepared using the ABAQUS/Standard software program. The essential details of the structure geometry were taken from the 3D scan of the vault. The first variant of the masonry vault was the structure without any strengthening, whereas the second variant was with strengthening system realized by application on composite materials, i.e., the carbon fiber reinforced cementitious matrix (C-FRCM). The results of the dynamic analysis revealed that an evident nonlinear performance of the masonry materials of the vault in both cases was detected for both FE models of the structure. The analysis proved that the foreshock–mainshock–aftershock sequence caused substantial damages in structural parts of the masonry vault. The distribution of plastic strains and damages allowed assessment of the impact of the full seismic sequence on the masonry vault. In the case of the unstrengthen vault the level of cracking and stiffness loss reached 90%. In the case of the vault strengthened with the FRCM system the tensile damage level was significantly lower. It did not exceed 30%. In addition, the first plastic zone of the unstrengthened masonry structural elements of the vault became visible after the foreshock.
Effect of Granulometric Composition of the Soil on the Occurrence of Carrion Beetles (Coleoptera: Silphidae)
Authors: Konieczna Karolina , Czerniakowski Zbigniew W. , Szostek Małgorzata
Published: January 2021

The entomological material was collected in the years 2009–2012 and 2014 from 13 different habitat types from three localities in south-eastern Poland. In total, 11,095 Silphidae were collected. This study examined whether the percentage of individual soil granulometric fractions was significantly related to the total abundance of collected Silphidae and individual carrion beetle species. A positive correlation and a statistically significant correlation were found between the total number of specimens collected and the share of the mechanical fraction with a diameter of 0.05–0.002 mm (silt fraction). In three species, a statistically significant correlation was demonstrated between the number of collected Silphidae and the share of the mechanical fraction with a diameter of 2–0.05 mm (sand fraction). The two species Phosphuga atrata atrata and Nicrophorus vespilloides the correlation was positive. A statistically significant relationship was also observed for Thanatophilus sinuatus, but the correlation was negative. With regard to the mechanical fraction with a diameter <0.002 mm (clay fraction), a statistically significant relationship was demonstrated for Oiceoptoma thoracicum and for Nicrophorus vespilloides for which the correlation was negative. However, a positive correlation was found for T. sinuatus in this case.
Compact Beam Homogenizer Module with Laser-Fabricated Lens-Arrays
Authors: Schwarz Simon , Götzendorfer Babette , Rung Stefan , Esen Cemal , Hellmann Ralf
Published: January 2021

We report on manufacturing of a compact beam homogenizer module including two lens arrays and an aperture. Lens arrays are fabricated by an all laser-based technology employing a precise femtosecond pulsed laser ablation and a CO2 laser polishing step. Each lens array is processed revealing a high contour accuracy and a roughness of 25 nm. The 8x8 lens arrays are designed to have a square footprint to generate a quadratic Top-Hat beam profile and focal length of 10 mm to realize compact packaging. Firstly, the lens arrays are tested in an experimental setup using commercial lens holders with their functionality being demonstrated by shaping a uniform 4.5 mm squared Top-Hat beam profile, as being calculated. Afterwards, a 3D printer is used to additively manufacture the housing for the beam homogenizer module having a length of only 16 mm. After assembling the laser-fabricated lens arrays and a laser-cutted aperture into the housing, the functionality of the miniaturized module is proven.