bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Myco-Biocontrol Based Formulates on Yield, Physiology and Secondary Products of Organically Grown Basil
Authors: Teliban Gabriel-Ciprian , Burducea Marian , Zheljazkov Valtcho D. , Dincheva Ivayla , Badjakov Ilian , Munteanu Neculai , Mihalache Gabriela , Cojocaru Alexandru , Popa Lorena-Diana , Stoleru Vasile
Published: February 2021

The development of organic farming as a result of increasing consumer preference for organic food has led to the development and registration of new pest-control products for certified organic production. In this study, the effects of three biocontrol products containing spores and mycelium of Arthrobotrys oligospora—Artis®, Beauveria bassiana—Bora®, and Coniothyrium minitans—Öko-ni® were tested on four basil (Ocimum basilicum L.) cultivars: ‘Aromat de Buzau’, ‘Serafim’, ‘Macedon’ and ‘Cuisoare’. The application of Öko-ni® increased basil yields by 8% relative to Control. The application of Bora® increased chlorophyll content of basil leaves by 2% and the activity of photosynthesis by 66% relative to the Control. Basil essential oil (EO) content was increased by 18% with the application of Artis® and by 34% with the application of Bora® and Öko-ni®, respectively. The content of phenolic compounds analyzed by HPLC varied; caffeic acid concentration was higher in the plants treated with Öko-ni®, hyperoside, isoquercitrin and rutin concentrations were higher in those treated with Artis®, while the quercitrin content was higher in Bora®-treated plants. The two main EO constituents that were identified were linalool and methyl chavicol in ‘Aromat de Buzau’, linalool and eugenol in ‘Serafim’, neral and geranial in ‘Macedon’, also linalool and eugenol in ‘Cuisoare’. The investigated myco-biocontrol products had positive effects on basil fresh biomass and EO content and also influenced the content of phenolic compounds.
Fertilization Management Improves the Yield and Capsaicinoid Content of Chili Peppers
Authors: Stan Teodor , Munteanu Neculai , Teliban Gabriel-Ciprian , Cojocaru Alexandru , Stoleru Vasile
Published: February 2021

Chili, one of the most cultivated plants in the world, from the genus Capsicum sp., has great importance both in human nutrition and in the pharmaceutical industry. This study provides detailed information on the impact of chili crop fertilization on the production and accumulation of capsaicin and dihydrocapsaicin. During the vegetation period, 235 kg∙ha−1 NPK (chemical—Ch), 270 kg∙ha−1 NPK (organic—O) and 250 kg∙ha−1 NPK (mixed—Ch + O) fertilizers were applied on six varieties of chili pepper (De Cayenne, Traian 2, Turkish, Sigaretta di Bergamo, Jovial and Chorbadjiiski); all versions were compared with the control (Ct). The determination of capsaicinoid compounds from chili pepper samples was done using high-performance liquid chromatography, HPLC-UV/VIS. The chili pepper plants reacted differently according to the fertilizers used, both in terms of the production and accumulation of capsaicinoids. The highest production was obtained for the case of mixed treatments in all cultivars, with the highest production being found for Sigaretta di Bergamo (40.61 t∙ha−1). The capsaicin and dihydrocapsaicin content was influenced by both the type of fertilizer used and the variety of chili pepper. The accumulation of capsaicinoids in the chili fruits was found to be dependent on cultivar and fertilization management; higher amounts of capsaicinoids were found to accumulate in the fruits of the Chorbadjiiski variety treated with chemicals (0.83 mg∙g−1 capsaicin and 0.53 mg∙g−1 dihydrocapsaicin) compared with the amounts found for untreated De Cayenne (0.52 mg∙g−1 capsaicin and 0.33 mg∙g−1 dihydrocapsaicin).
Exogenous Serotonin Improves Salt Tolerance in Rapeseed (Brassica napus L.) Seedlings
Authors: Liu Yue , Ding Xiaoyu , Lv Yan , Cheng Yong , Li Chunsheng , Yan Lei , Tian Shanshan , Zou Xiling
Published: February 2021

Serotonin is a well-known agent that plays various roles in animals, and is little known in plants. In this study, the effect of exogenous serotonin was tested on Brassica napus L. (rapeseed) under salt stress. The results revealed that exogenous application of 200 µmol/L serotonin had the best protection under salinity. Exogenous serotonin effectively alleviated the growth inhibition of seedlings caused by salinity, and significantly promoted the accumulation of the fresh and dry weights of roots and shoots. Besides, although the H2O2 and malondialdehyde (MDA) contents were raised under salinity, they were reduced by exogenous serotonin. The chlorophyll content was decreased under salinity, and was increased by exogenous serotonin. Under salinity, serotonin effectively activated antioxidant enzyme system through improving the catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities, and the expression of POD7, CAT3 and Cu-SOD genes was also up-regulated. The results also revealed exogenous serotonin increased the solute content by promoting the accumulation of soluble sugar and protein. In conclusion, salinity caused a toxicity to seedlings through oxidative damage to chlorophyll and cell membrane integrity, and serotonin possessed the ability of scavenging reactive oxygen species, osmotic pressure regulation and promoting growth, thus alleviating salinity of rape seedlings.
Development of an Integrated IoT-Based Greenhouse Control Three-Device Robotic System
Authors: Thomopoulos Vasileios , Bitas Dionysios , Papastavros Kyriakos-Nikos , Tsipianitis Dimitris , Kavga Angeliki
Published: February 2021

The control of large greenhouse installations, especially those with hydroponics crops, is based on the analysis and use of data recorded by many sensors. At the same time, the size of such installations does not allow for their effective terrestrial surveillance, to detect problems promptly. In recent years, there has been an interest in the development of autonomous agbots equipped with agricultural sensors. Several ground-based AGV (automated guided vehicles) and UAV (unmanned aerial vehicles) systems have been developed for use in open-air plots. A key feature of all these innovative systems is spectroscopy, the development of which has been assisted by the surveillance capabilities and speed of modern-day UAVs (drones). However, there is a lag in the use of spectroscopy inside greenhouses since UAVs do not move freely indoors. In this paper, we propose as a solution a three-device (3DS) system.
Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles
Authors: Lee Seok Hee , Lira-Albarrán Saúl , Saadeldin Islam M
Published: February 2021

Dogs (Canis lupus familiaris) have unique and peculiar reproductive characteristics. While the interplay between in vitro oviductal cell-derived extracellular vesicles (OC-EVs) and cumulus-oocyte complexes in dogs has begun to be elucidated, no study has yet provided extensive information on the biological content and physiological function of OC-EVs and their role in canine oocyte development. Here, we aimed to provide the first comprehensive proteomic analysis of OC-EVs. We identified 398 proteins as present in all OC-EVs samples. The functional enrichment analysis using Gene Ontology terms and an Ingenuity Pathway Analysis revealed that the identified proteins were involved in several cellular metabolic processes, including translation, synthesis, expression, and protein metabolism. Notably, the proteins were also involved in critical canonical pathways with essential functions in oocyte and embryo development, such as ERK/MAPK, EIF2, PI3K/AKT, and mTOR signaling. These data would be an important resource for studying canine reproductive physiology and establishing a successful in vitro embryo production system in dogs.
Alternative Management Systems of Beef Cattle Manure for Reducing Nitrogen Loadings: A Case-Study Approach
Authors: Wang Yubin , Ghimire Suraj , Wang Jingjing , Dong Renjie , Li Qian
Published: February 2021

Livestock manure is one of the main sources of agricultural nonpoint source pollution and poses a great threat to the environment and human health. Sustainable management of manure via recycling is an effective means to tackle the problem. Based on field interviews in China, multiple case studies were employed to investigate alternative manure management systems. Four conclusions arose. First, compost-based systems, product-based systems, substrate-based systems, and biogas-based systems were identified as four main types of manure management systems, with each possessing its success factors and risk factors. The adoption of a system was driven by various factors. Second, market-oriented operation was the dominant operation mechanism of all the manure management systems. Third, compared to direct application of manure to croplands, all the four manure management systems could reduce nitrogen loadings from livestock farms and lower their environmental effects. Among the systems, biogas-based systems could reduce nitrogen loadings to the greatest extent, followed by product-based systems and substrate-based systems, and then by compost-based systems. Lastly, integrated management of manure with mixed recycling systems is imperative for reducing its environmental effects, which can benefit from the increasing role of third-party entities in manure recycling. Policy implications were also discussed.
Biometric Analysis of Cranial and Somatic Features in the Pannonian Root Vole
Authors: Baláž Ivan , Tulis Filip , Ševčík Michal
Published: February 2021

The Pannonian root vole Alexandromys oeconomus ssp. mehelyi represents a rare glacial relict, whose occurrence is nowadays bound to several areas in Europe. Four somatic and 25 craniological features were analysed, based on 355 measured specimens. Sex is a significant factor affecting the average value of all four somatic features, where all of them achieve higher values in males than in females. While body length and tail length were also affected by seasons, body weight and the length of the hind foot were stable features present across the seasons. In cranial features, the largest variability in the adult population is characterised by neurocranium breadth (LaN), total length of the cranial base (LB), and skull (LCr); whereas the smallest variability of the cranial dimensions is reflected in the values of the greatest palatal breadth (PS) and postorbital breadth (Io). Calculating the weight from cranial remains may be used to estimate the size of the prey and to determine vole biomass consumed by predators, such as raptors, highlighting the utility of studying feeding ecology.
Pharmacological Potential and Chemical Characterization of Bridelia ferruginea Benth.—A Native Tropical African Medicinal Plant
Authors: Mahomoodally Mohamad Fawzi , Jugreet Sharmeen , Sinan Kouadio Ibrahime , Zengin Gokhan , Ak Gunes , Ceylan Ramazan , Jekő József , Cziáky Zoltán , Angelini Paola , Angeles Flores Giancarlo , Venanzoni Roberto , Di Simone Simonetta Cristina , Menghini Luigi , Orlando Giustino , Ferrante Claudio , Etienne Ouattara Katinan , Tacchini Massimo
Published: February 2021

To avail the possible pharmacological actions of Brideliaferruginea Benth., the present investigation was designed to quantitatively analyze the total flavonoid and phenolic contents and assess the various antioxidant and enzyme inhibition properties of leaf and stem bark extracts (ethyl acetate, water and methanolic) of B. ferruginea. Anti-proliferative effect was also investigated against human colon cancer cells (HCT116) as well as the antimicrobial potential against multiple bacterial and fungal (yeasts and dermatophytes) strains. The methanolic and water extracts of the stem bark demonstrated the highest phenolic content (193.58 ± 0.98 and 187.84 ± 1.88 mg/g, respectively), while the leaf extracts showed comparatively higher flavonoid contents (24.37–42.31 mg/g). Overall, the methanolic extracts were found to possess the most significant antioxidant potency. Compared to the other extracts, methanolic extracts of the B. ferruginea were revealed to be most potent inhibitors of acetyl- and butyryl-cholinesterases, tyrosinase α-amylase, except α-glucosidase. Only the ethyl acetate extracts were found to inhibit glucosidase. Additionally, the stem bark methanolic extract also showed potent inhibitory activity against E. coli and gram-positive bacteria (MIC (minimum inhibitory concentration): 2.48–62.99 µg/mL), as well as all the tested fungi (MIC: 4.96–62.99 µg/mL). In conclusion, B. ferruginea can be regarded as a promising source of bioactive compounds displaying multifunctional pharmacological activities and thus is a potential candidate for further investigations in the endeavor to develop botanical formulations for pharmaceutical and cosmeceutical industries.
Entropy Generation and MHD Convection within an Inclined Trapezoidal Heated by Triangular Fin and Filled by a Variable Porous Media
Authors: Almuhtady Ahmad , Alhazmi Muflih , Al-Kouz Wael , Raizah Zehba A. S. , Ahmed Sameh E.
Published: February 2021

Analyses of the entropy of a thermal system that consists of an inclined trapezoidal geometry heated by a triangular fin are performed. The domain is filled by variable porosity and permeability porous materials and the working mixture is Al2O3-Cu hybrid nanofluids. The porosity is varied exponentially with the smallest distance to the nearest wall and the permeability is depending on the particle diameter. Because of using the two energy equations model (LTNEM), sources of the entropy are entropy due to the transfer of heat of the fluid phase, entropy due to the fluid friction and entropy due to the porous phase transfer of heat. A computational domain with new coordinates (ξ,η) is created and Finite Volume Method (FVM) in case of the non-orthogonal grids is used to solve the resulting system. Various simulations for different values of the inclination angle, Hartmann number and alumina-copper concentration are carried out and the outcomes are presented in terms of streamlines, temperature, fluid friction entropy and Bejan number. It is remarkable that the increase in the inclination angle causes a diminishing of the heat transfer rate. Additionally, the irreversibility due to the temperature gradients is dominant near the heated fins, regardless of the values of the Hartmann number.
Special Issue: The State of the Art of Thermochemical Heat Storage
Authors: Vasta Salvatore
Published: February 2021

Nowadays, thermal energy storage (TES) is gaining a crucial role in the development of highly efficient thermal energy systems [...]
Data Assimilation of AOD and Estimation of Surface Particulate Matters over the Arctic
Authors: Han Kyung M. , Jung Chang H. , Park Rae-Seol , Park Soon-Young , Lee Sojin , Kulmala Markku , Petäjä Tuukka , Karasiński Grzegorz , Sobolewski Piotr , Yoon Young Jun , Lee Bang Young , Kim Kiyeon , Kim Hyun S.
Published: February 2021

In this study, more accurate information on the levels of aerosol optical depth (AOD) was calculated from the assimilation of the modeled AOD based on the optimal interpolation method. Additionally, more realistic levels of surface particulate matters over the Arctic were estimated using the assimilated AOD based on the linear relationship between the particulate matters and AODs. In comparison to the MODIS observation, the assimilated AOD was much improved compared with the modeled AOD (e.g., increase in correlation coefficients from −0.15–0.26 to 0.17–0.76 over the Arctic). The newly inferred monthly averages of PM10 and PM2.5 for April–September 2008 were 2.18–3.70 μg m−3 and 0.85–1.68 μg m−3 over the Arctic, respectively. These corresponded to an increase of 140–180%, compared with the modeled PMs. In comparison to in-situ observation, the inferred PMs showed better performances than those from the simulations, particularly at Hyytiala station. Therefore, combining the model simulation and data assimilation provided more accurate concentrations of AOD, PM10, and PM2.5 than those only calculated from the model simulations.
Numerical Study of Meteorological Factors for Tropospheric Nocturnal Ozone Increase in the Metropolitan Area of São Paulo
Authors: Urbina Guerrero Viviana Vanesa , Morais Marcos Vinicius Bueno de , Freitas Edmilson Dias de , Martins Leila Droprinchinski
Published: February 2021

One of the central problems in large cities is air pollution, mainly caused by vehicular emissions. Tropospheric ozone is an atmospheric oxidizing gas that forms in minimal amounts naturally, affecting peoples’ health. This pollutant is formed by the NO2 photolysis, creating a main peak during the day. Nighttime secondary peaks occur in several parts of the world, but their intensity and frequency depend on the local condition. In this sense, this works aims to study the local characteristics for tropospheric nocturnal ozone levels in the Metropolitan Area of São Paulo, in Brazil, using the Simple Photochemical Module coupled to the Brazilian Developments on the Regional Atmospheric Modeling System. For this, three different situations of nocturnal occurrence were studied. The results show that the nocturnal maximum of ozone concentrations is related to the vertical transport of this pollutant from higher levels of the atmosphere to the surface and is not related to the synoptic condition.
Effects of PM10 and Weather on Respiratory and Cardiovascular Diseases in the Ciuc Basin (Romanian Carpathians)
Authors: Bodor Katalin , Micheu Miruna Mihaela , Keresztesi Ágnes , Birsan Marius-Victor , Nita Ion-Andrei , Bodor Zsolt , Petres Sándor , Korodi Attila , Szép Róbert
Published: February 2021

This study presents the PM10 concentration, respiratory and cardiovascular disease hospital admissions evolution in the Ciuc basin for a period of 9 years (2008–2016), taking into consideration different meteorological conditions: boundary layer, lifting condensation level, temperature-humidity index, and wind chill equivalent chart index. The PM10 and hospital admissions evolution showed a very fluctuated hourly, weekly, monthly, yearly tendency. The PM10 concentration in winter (34.72 μg/m3) was 82% higher than the multiannual average (19.00 μg/m3), and almost three times higher than in summer (11.71 μg/m3). During the winter, PM10 concentration increased by an average of 9.36 μg/m3 due to the increased household heating. Climatological parameters have a demonstrable effect on the PM10 concentration variation. Children, the elderly and men are more sensitive to air pollution, the calculated relative risk for men was (RR = 1.45), and for women (RR = 1.37), respectively. A moderate correlation (0.51) was found between PM10 and pneumonia (P), while a relatively weak correlation (0.39) was demonstrated in the case of PM10 and upper respiratory tract infections (URTI). Furthermore, except thermal humidity index (THI), strong negative correlations were observed between the multiannual monthly mean PM10 and the meteorological data. The PM10 followed a moderate negative correlation with the boundary layer (−0.61). In the case of URTI and P, the highest number of hospital admissions occurred with a 5 to 7-day lag, while the 10 μg/m3 PM10 increase resulted in a 2.04% and 8.28% morbidity increase. For lung cancer (LC) and cardiovascular diseases (AMI, IHD, CCP), a maximum delay of 5–6 months was found. Three-month delay and an average growth of 1.51% was observed in the case of chronic obstructive pulmonary disease (COPD). Overall, these findings revealed that PM10 was and it is responsible for one-third of the diseases.
Advantages of Additive Manufacturing for Biomedical Applications of Polyhydroxyalkanoates
Authors: Giubilini Alberto , Bondioli Federica , Messori Massimo , Nyström Gustav , Siqueira Gilberto
Published: February 2021

In recent years, biopolymers have been attracting the attention of researchers and specialists from different fields, including biotechnology, material science, engineering, and medicine. The reason is the possibility of combining sustainability with scientific and technological progress. This is an extremely broad research topic, and a distinction has to be made among different classes and types of biopolymers. Polyhydroxyalkanoate (PHA) is a particular family of polyesters, synthetized by microorganisms under unbalanced growth conditions, making them both bio-based and biodegradable polymers with a thermoplastic behavior. Recently, PHAs were used more intensively in biomedical applications because of their tunable mechanical properties, cytocompatibility, adhesion for cells, and controllable biodegradability. Similarly, the 3D-printing technologies show increasing potential in this particular field of application, due to their advantages in tailor-made design, rapid prototyping, and manufacturing of complex structures. In this review, first, the synthesis and the production of PHAs are described, and different production techniques of medical implants are compared. Then, an overview is given on the most recent and relevant medical applications of PHA for drug delivery, vessel stenting, and tissue engineering. A special focus is reserved for the innovations brought by the introduction of additive manufacturing in this field, as compared to the traditional techniques. All of these advances are expected to have important scientific and commercial applications in the near future.
High-Throughput Screening Platforms in the Discovery of Novel Drugs for Neurodegenerative Diseases
Authors: Aldewachi Hasan , Al-Zidan Radhwan N. , Conner Matthew T. , Salman Mootaz M.
Published: February 2021

Neurodegenerative diseases (NDDs) are incurable and debilitating conditions that result in progressive degeneration and/or death of nerve cells in the central nervous system (CNS). Identification of viable therapeutic targets and new treatments for CNS disorders and in particular, for NDDs is a major challenge in the field of drug discovery. These difficulties can be attributed to the diversity of cells involved, extreme complexity of the neural circuits, the limited capacity for tissue regeneration, and our incomplete understanding of the underlying pathological processes. Drug discovery is a complex and multidisciplinary process. The screening attrition rate in current drug discovery protocols mean that only one viable drug may arise from millions of screened compounds resulting in the need to improve discovery technologies and protocols to address the multiple causes of attrition. This has identified the need to screen larger libraries where the use of efficient high-throughput screening (HTS) becomes key in the discovery process. HTS can investigate hundreds of thousands of compounds per day. However, if fewer compounds could be screened without compromising the probability of success, the cost and time would be largely reduced. To that end, recent advances in computer-aided design, in silico libraries, and molecular docking software combined with the upscaling of cell-based platforms have evolved to improve screening efficiency with higher predictability and clinical applicability. We review, here, the increasing role of HTS in contemporary drug discovery processes, in particular for NDDs, and evaluate the criteria underlying its successful application. We also discuss the requirement of HTS for novel NDD therapies and examine the major current challenges in validating new drug targets and developing new treatments for NDDs.
Systemic Photoprotection in Skin Cancer Prevention: Knowledge among Dermatologists
Authors: Fania Luca , Sampogna Francesca , Ricci Francesco , Hyeraci Mariafrancesca , Paradisi Andrea , Palese Enzo , Di Lella Giovanni , Pallotta Sabatino , Panebianco Annarita , Candi Eleonora , Dellambra Elena , Abeni Damiano
Published: February 2021

Background: Systemic photoprotection (i.e., administration of substances such as nicotinamide, carotenoids, and vitamin D) may be important to reduce photocarcinogenesis or to support long-term protection against UV irradiation. Clinical trials showed that oral nicotinamide is effective in reducing the onset of new nonmelanoma skin cancers (NMSCs), while other oral photoprotectors failed to achieve the reduction of new melanoma or NMSC formation in humans. The aim of this study was to summarize the current state of knowledge of systemic photoprotection and to evaluate the knowledge and attitude of dermatologists regarding these treatments. Methods: The survey was conducted on a sample of dermatologists recruited according to a snowball sampling procedure. The questionnaire consisted of a first part asking for characteristics of the participant and a second part with 12 specific questions on their knowledge about systemic photoprotection, particularly their knowledge of astaxanthin, β-carotene, nicotinamide, and vitamin D3. Results: One hundred eight dermatologists answered the survey. Most of them (85.2%) stated that oral photoprotectors have a role in the prevention of skin cancer, and responses mainly mentioned nicotinamide. More than half of them (54.6%) had prescribed all the considered oral photoprotectors, but the majority of them had prescribed nicotinamide, mainly for 2 to 3 months during summer, almost invariably (n = 106) associated with topical photoprotectors. Most dermatologists (>80%) were aware of scientific publications demonstrating an effect of systemic photoprotectors on NMSC. Conclusions: Most Italian dermatologists have positive views on oral photoprotection in skin cancer and are aware of the demonstrated potential of nicotinamide in the prevention of NMSCs.
Actin Cytoskeleton and Regulation of TGFβ Signaling: Exploring Their Links
Authors: Melchionna Roberta , Trono Paola , Tocci Annalisa , Nisticò Paola
Published: February 2021

Human tissues, to maintain their architecture and function, respond to injuries by activating intricate biochemical and physical mechanisms that regulates intercellular communication crucial in maintaining tissue homeostasis. Coordination of the communication occurs through the activity of different actin cytoskeletal regulators, physically connected to extracellular matrix through integrins, generating a platform of biochemical and biomechanical signaling that is deregulated in cancer. Among the major pathways, a controller of cellular functions is the cytokine transforming growth factor β (TGFβ), which remains a complex and central signaling network still to be interpreted and explained in cancer progression. Here, we discuss the link between actin dynamics and TGFβ signaling with the aim of exploring their aberrant interaction in cancer.
Neuroprogression as an Illness Trajectory in Bipolar Disorder: A Selective Review of the Current Literature
Authors: Serafini Gianluca , Pardini Matteo , Monacelli Fiammetta , Orso Beatrice , Girtler Nicola , Brugnolo Andrea , Amore Mario , Nobili Flavio ,   Team on Dementia of the IRCCS Ospedale Policlinico San Martino
Published: February 2021

Bipolar disorder (BD) is a chronic and disabling psychiatric condition that is linked to significant disability and psychosocial impairment. Although current neuropsychological, molecular, and neuroimaging evidence support the existence of neuroprogression and its effects on the course and outcome of this condition, whether and to what extent neuroprogressive changes may impact the illness trajectory is still poorly understood. Thus, this selective review was aimed toward comprehensively and critically investigating the link between BD and neurodegeneration based on the currently available evidence. According to the most relevant findings of the present review, most of the existing neuropsychological, neuroimaging, and molecular evidence demonstrates the existence of neuroprogression, at least in a subgroup of BD patients. These studies mainly focused on the most relevant effects of neuroprogression on the course and outcome of BD. The main implications of this assumption are discussed in light of specific shortcomings/limitations, such as the inability to carry out a meta-analysis, the inclusion of studies with small sample sizes, retrospective study designs, and different longitudinal investigations at various time points.
Augmented Reality to Enable Users to Identify Deviations for Model Reconciliation
Authors: Chalhoub Jad , Ayer Steven K. , McCord Kieren H.
Published: February 2021

Effective use of Building Information Modeling (BIM) during operation typically requires modeled content to accurately match the built spaces, which necessitates effective field verification techniques to ensure that BIM content matches the actual built conditions. Some contractors leverage laser scanning and other reality-capture technologies to verify modeled content prior to turnover, but these approaches can be time- and resource-intensive. Augmented reality (AR) enables users to view BIM content overlaid on their field of view of the built space. Research suggests potential for using AR for tasks related to field verification, but a study that systematically explores the specific types of deviations that can be detected with this technology is missing from the current literature. This paper tasks participants with using immersive AR to identify deviations from BIM in a ceiling plenum space that includes installed Mechanical, Electrical and Plumbing (MEP) components, which would typically be included in a coordinated BIM. The results suggest that AR can enable users to effectively identify large deviations and missing building elements. However, the results do not indicate that AR effectively enables users to identify small deviations and can potentially lead to identifying false positive observations, where accurately constructed elements are perceived as deviating from BIM. These results suggest that immersive AR can effectively be used to check whether recently built elements conform to the intended BIM in instances where speed of verification is more important than adhering to strict tolerances. For instances where accuracy (less than two inches) is critical, the results of this work suggest that AR can be used to help determine where in the building more accurate, but more resource-intensive, reality-capture technologies should be used.
A 10-Year Probability Deep Neural Network Prediction Model for Lung Cancer
Authors: Lee Hsiu-An , Chao Louis R. , Hsu Chien-Yeh
Published: February 2021

Cancer is the leading cause of death in Taiwan. According to the Cancer Registration Report of Taiwan’s Ministry of Health and Welfare, a total of 13,488 people suffered from lung cancer in 2016, making it the second-most common cancer and the leading cancer in men. Compared with other types of cancer, the incidence of lung cancer is high. In this study, the National Health Insurance Research Database (NHIRDB) was used to determine the diseases and symptoms associated with lung cancer, and a 10-year probability deep neural network prediction model for lung cancer was developed. The proposed model could allow patients with a high risk of lung cancer to receive an earlier diagnosis and support the physicians’ clinical decision-making. The study was designed as a cohort study. The subjects were patients who were diagnosed with lung cancer between 2000 and 2009, and the patients’ disease histories were back-tracked for a period, extending to ten years before the diagnosis of lung cancer. As a result, a total of 13 diseases were selected as the predicting factors. A nine layers deep neural network model was created to predict the probability of lung cancer, depending on the different pre-diagnosed diseases, and to benefit the earlier detection of lung cancer in potential patients. The model is trained 1000 times, the batch size is set to 100, the SGD (Stochastic gradient descent) optimizer is used, the learning rate is set to 0.1, and the momentum is set to 0.1. The proposed model showed an accuracy of 85.4%, a sensitivity of 72.4% and a specificity of 85%, as well as an 87.4% area under ROC (AUROC) (95%, 0.8604–0.8885) model precision. Based on data analysis and deep learning, our prediction model discovered some features that had not been previously identified by clinical knowledge. This study tracks a decade of clinical diagnostic records to identify possible symptoms and comorbidities of lung cancer, allows early prediction of the disease, and assists more patients with early diagnosis.
Heterogeneous Ru Catalysts as the Emerging Potential Superior Catalysts in the Selective Hydrogenation of Bio-Derived Levulinic Acid to γ-Valerolactone: Effect of Particle Size, Solvent, and Support on Activity, Stability, and Selectivity
Authors: Maumela Mulisa , Marx Sanette , Meijboom Reinout
Published: February 2021

Catalytic hydrogenation of a biomass-derived molecule, levulinic acid (LA), to γ-valerolactone (GVL) has been getting much attention from researchers across the globe recently. This is because GVL has been identified as one of the potential molecules for replacing fossil fuels. For instance, GVL can be catalytically converted into liquid alkenes in the molecular weight range close to that found in transportation fuels via a process that does not require an external hydrogen source. Noble and non-noble metals have been used as catalysts for the selective hydrogenation of LA to GVL. Of these, Ru has been reported to be the most active metal for this reaction. The type of metal supports and solvents has been proved to affect the activity, selectivity, and yields of GVL. Water has been identified as a potential, effective “green” solvent for the hydrogenation of LA to GVL. The use of different sources of H2 other than molecular hydrogen (such as formic acid) has also been explored. In a few instances, the product, GVL, is hydrogenated further to other useful products such as 1,4-pentanediol (PD) and methyl tetrahydrofuran (MTHF). This review selectively focuses on the potential of immobilized Ru catalysts as a potential superior catalyst for selective hydrogenation of LA to GVL.
IQGAP1 Is a Scaffold of the Core Proteins of the Hippo Pathway and Negatively Regulates the Pro-Apoptotic Signal Mediated by This Pathway
Authors: Quinn Niall P. , García-Gutiérrez Lucía , Doherty Carolanne , von Kriegsheim Alexander , Fallahi Emma , Sacks David B. , Matallanas David
Published: February 2021

The Hippo pathway regulates a complex signalling network which mediates several biological functions including cell proliferation, organ size and apoptosis. Several scaffold proteins regulate the crosstalk of the members of the pathway with other signalling pathways and play an important role in the diverse output controlled by this pathway. In this study we have identified the scaffold protein IQGAP1 as a novel interactor of the core kinases of the Hippo pathway, MST2 and LATS1. Our results indicate that IQGAP1 scaffolds MST2 and LATS1 supresses their kinase activity and YAP1-dependent transcription. Additionally, we show that IQGAP1 is a negative regulator of the non-canonical pro-apoptotic pathway and may enable the crosstalk between this pathway and the ERK and AKT signalling modules. Our data also show that bile acids regulate the IQGAP1-MST2-LATS1 signalling module in hepatocellular carcinoma cells, which could be necessary for the inhibition of MST2-dependent apoptosis and hepatocyte transformation.
Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces
Authors: Mucientes Arkaitz , Herranz Eva , Moro Enrique , González-Corchón Aranzazu , Peña-Soria María Jesús , Abasolo Lydia , Rodriguez-Rodriguez Luis , Lamas Jose Ramon , Fernández-Gutiérrez Benjamín
Published: February 2021

Current gold-standard strategies for bone regeneration do not achieve the optimal recovery of bone biomechanical properties. To bypass these limitations, tissue engineering techniques based on hybrid materials made up of osteoprogenitor cells—such as mesenchymal stem cells (MSCs)—and bioactive ceramic scaffolds—such as calcium phosphate-based (CaPs) bioceramics—seem promising. The biological properties of MSCs are influenced by the tissue source. This study aims to define the optimal MSC source and construct (i.e., the MSC–CaP combination) for clinical application in bone regeneration. A previous iTRAQ analysis generated the hypothesis that anatomical proximity to bone has a direct effect on MSC phenotype. MSCs were isolated from adipose tissue, bone marrow, and dental pulp, then cultured both on a plastic surface and on CaPs (hydroxyapatite and β-tricalcium phosphate), to compare their biological features. On plastic, MSCs isolated from dental pulp (DPSCs) presented the highest proliferation capacity and the greatest osteogenic potential. On both CaPs, DPSCs demonstrated the greatest capacity to colonise the bioceramics. Furthermore, the results demonstrated a trend that DPSCs had the most robust increase in ALP activity. Regarding CaPs, β-tricalcium phosphate obtained the best viability results, while hydroxyapatite had the highest ALP activity values. Therefore, we propose DPSCs as suitable MSCs for cell-based bone regeneration strategies.
Special Issue “Surface Modification of Metals and Alloys”
Authors: Rius-Ayra Oriol , Llorca-Isern Nuria
Published: February 2021

Recently surface modification has become necessary for the scientific community because of surface properties of new materials are usually inadequate in terms of wettability, adhesion, corrosion resistance or even drag reduction [...]
Intention to Purchase Halal Cosmetics: Do Males and Females Differ? A Multigroup Analysis
Authors: Ngah Abdul Hafaz , Gabarre Serge , Han Heesup , Rahi Samar , Al-Gasawneh Jassim Ahmad , Park Su-hyun
Published: February 2021

As Muslims bound to Islamic teachings, the attitude of young millennials preferring non-halal international cosmetics is trivial. Despite the acceptance of halal food, literature on the acceptance of halal cosmetics remains scarce. The intention to purchase halal cosmetics is crucial for the sustainability of halal cosmetics manufacturers. The authors used the theory of planned behavior to identify factors influencing the purchase intention of halal cosmetics among Muslim millennials. Since cosmetics are not exclusively used by females, as males are starting to use them in their daily lives, gender was incorporated into the framework to assess its moderating effect on the relationship. Furthermore, brand image was included in the theory of planned behavior. Data were collected from three universities in Malaysia. A total of 501 responses were analyzed with smart partial least squares to run a multigroup analysis. The analysis revealed that subjective norms have a stronger effect on females, and perceived behavioral control has a greater effect on males. Although attitude and brand image have a positive effect on the intention to purchase halal cosmetics, gender has no effect. The findings are essential for halal cosmetics manufacturers to craft a marketing strategy aimed at Muslim millennials in Malaysia.
Design and Realization of a Compact Efficient Beam Combiner, Based on Liquid Crystal Pancharatnam–Berry Phase Gratings
Authors: Gao Boxuan , Beeckman Jeroen , Neyts Kristiaan
Published: February 2021

We demonstrate a laser beam combiner based on four photo-patterned Pancharatnam–Berry (PB) phase gratings, which is compact and has high diffraction efficiency for incident circularly polarized light. The nematic liquid crystal mixture E7 is used as anisotropic material, and the thickness of the layer is controlled by spacers. The beam combiner can bring two parallel laser beams closer to each other while remaining parallel. This work shows the potential to realize components based on flat optical LC devices.
The Diverse Assemblage of Fungal Endophytes from Orchids in Madagascar Linked to Abiotic Factors and Seasonality
Authors: Yokoya Kazutomo , Zettler Lawrence W. , Bell Jake , Kendon Jonathan P. , Jacob Alison S. , Schofield Emily , Rajaovelona Landy , Sarasan Viswambharan
Published: February 2021

The inselbergs of the Central Highlands of Madagascar are one of many ‘micro-hotspots’ of biodiversity on the island, particularly for Orchidaceae. In this region are several genera that have a large number of endemic species that are in serious decline or edging towards extinction. Studies relating to diversity of orchids and their fungal partners (both mycorrhizal and non-mycorrhizal root associates) deserve more attention, as climate change and human induced decline in resilience of species in the wild is at an all-time high. Identification of mycorrhizal fungi (MF) via conventional seed baited-protocorms has limitations for large scale studies and its application for time-bound conservation projects. The paper describes the value of understanding fungal diversity in the roots of orchids at different stages of maturity. The first part of the study was a preliminary investigation mainly to identify culturable Rhizoctonia endophytes, and the second part looked at all life forms of available taxa together with associated soil characteristics. We isolated and identified 19 putative MF from 18 of the 50 taxa spread over an area of 250 sq. km, covering three life forms, growth phases of the orchid taxa, and habitat types. In the rest of the taxa, we were unable to detect any putative MF, but had varying numbers of non-mycorrhizal endophytes. We also found that diversity of putative MF was higher in plants from soils with the lowest P levels recorded. Putative mycorrhizal OTUs were predominantly from the Tulasnella lineage, followed by Ceratobasidium and Serendipita. Within a small subset of samples, a difference in colonised endophytes depending on the collection season was observed. In vitro germination studies using 10 OTUs of mycorrhizal fungi in 14 orchid species showed mostly generalist associations. When orchid seed and fungal sources were studied irrespective of habitat, life form, and distance from each other (orchid seed and fungal source), compatibility for symbiotic seed germination was observed in most cases. Issues with the identification of compatible MF and symbiotic system of seed germination are discussed.
Annelids in Extreme Aquatic Environments: Diversity, Adaptations and Evolution
Authors: Glasby Christopher J. , Erséus Christer , Martin Patrick
Published: February 2021

We review the variety of morphological, physiological and behavioral modifications that annelids have acquired to cope with environments either unsuitable for, or on the limits of, survival for most animals. We focus on polychaetes (excluding sipunculans and echiurans) and clitellates (oligochaetes and leeches) and source information mostly from the primary literature. We identified many modifications common to both polychaetes and clitellates, and others that are specific to one or the other group. For example, certain land-adapted polychaetes show reduction in nuchal organs, epidermal ciliation and receptor cells, and other coastal polychaetes use adhesive glands and glue-reinforced tubes to maintain position in surf zones, while oligochaetes, with their simple body plans, appear to be ‘pre-adapted’ to life underground. Modifications common to both groups include the ability to construct protective cocoons, make cryoprotective substances such as antifreeze and heat shock proteins, develop gills, transform their bodies into a home for symbiotic chemoautotrophic bacteria, metabolize contaminants, and display avoidance behaviors. Convergent evolution in both directions has enabled annelids to transition from salt water to freshwater, sea to land via beaches, freshwater to soil, and surface water to subterranean water. A superficially simple worm-like body and a mostly benthic/burrowing lifestyle has facilitated radiation into every conceivable environment, making annelids among the most common and diverse animal groups on the planet.
Acceptability and Usability of HCV Self-Testing in High Risk Populations in Vietnam
Authors: Nguyen Linh Thuy , Nguyen Van Thi Thuy , Le Ai Kim Anh , Truong Minh Binh , Tran Tam Thi Minh , Jamil Muhammad S. , Johnson Cheryl , Reipold Elena Ivanova , Easterbrook Philippa , Park Kidong
Published: February 2021

HIV self-testing has emerged as a safe and effective approach to increase the access to and uptake of HIV testing and treatment, especially for key populations. Applying self-testing to hepatitis C virus (HCV) may also offer an additional way to address low coverage of HCV testing and to accelerate elimination efforts. To understand the potential for HCV self-testing (HCVST), an observational study was conducted to assess the acceptability and usability of the OraQuick® HCV Self-Test (prototype) among people who inject drugs (PWID) and men who have sex with men (MSM) in Thai Nguyen, a province in northern Vietnam. A total of 105 PWID and 104 MSM were eligible and agreed to participate in the study. Acceptability, defined as the proportion of participants among eligible subjects who agreed to participate in the study, was 92.9% in PWID and 98.6% in MSM. Compared to MSM, PWID were older (median age: 45 vs. 22 years; p < 0.0001) and had a lower education level (high school and college: 38.1% vs. 100%; p < 0.0001). HCVST usability was high among MSM with fewer observed mistakes, difficulties, or participants requiring assistance (33.7%, 28.8%, and 17.3%, respectively) compared to PWID (62.9%, 53.3%, and 66.7%, respectively; all p < 0.0001)). Inter-reader and inter-operator agreement were good in both groups (Kappa coefficient range: 0.61–0.99). However, the concordance between HCVST and study staff -read or performed HCV testing was lower among PWID than MSM (inter-reader concordance 88.6% vs. 99.0% and inter-operator concordance 81.9% vs. 99%). Overall, HCVST was highly acceptable with moderate to high usability among PWID and MSM in Thai Nguyen. Efforts to provide support and assistance may be needed to optimize performance, particularly for PWID populations and for those who are older and with lower literacy or education levels.
An Improvised Machine Learning Model Based on Mutual Information Feature Selection Approach for Microbes Classification
Authors: Dhindsa Anaahat , Bhatia Sanjay , Agrawal Sunil , Sohi Balwinder Singh
Published: February 2021

The accurate classification of microbes is critical in today’s context for monitoring the ecological balance of a habitat. Hence, in this research work, a novel method to automate the process of identifying microorganisms has been implemented. To extract the bodies of microorganisms accurately, a generalized segmentation mechanism which consists of a combination of convolution filter (Kirsch) and a variance-based pixel clustering algorithm (Otsu) is proposed. With exhaustive corroboration, a set of twenty-five features were identified to map the characteristics and morphology for all kinds of microbes. Multiple techniques for feature selection were tested and it was found that mutual information (MI)-based models gave the best performance. Exhaustive hyperparameter tuning of multilayer layer perceptron (MLP), k-nearest neighbors (KNN), quadratic discriminant analysis (QDA), logistic regression (LR), and support vector machine (SVM) was done. It was found that SVM radial required further improvisation to attain a maximum possible level of accuracy. Comparative analysis between SVM and improvised SVM (ISVM) through a 10-fold cross validation method ultimately showed that ISVM resulted in a 2% higher performance in terms of accuracy (98.2%), precision (98.2%), recall (98.1%), and F1 score (98.1%).