bluuebunny/crossref_metadata_2025 huggingface dataset:
A qualitative study of how COVID-19 impacts on Australians’ hopes and dreams
Authors: Huang Quincy F. , Rolf Floraidh , Booker Lauren A. , Moore Taleah , Thompson Sandra C.
Published: February 2022

Background

Although beginning in 2019, it was early in 2020 that the global community began to comprehend the significant impact that a pandemic of a new coronavirus might have on their own lives. This study was undertaken 6–9 months after significant public health restrictions were introduced within Australia and examined the impact of the COVID-19 on individuals’ hopes and dreams for their future.

Methods

Community members who responded to a survey about COVID-19 were invited to participate in follow up interviews if they reported living with a chronic condition. Participants across Australia who consented were interviewed between August and December in 2020 over telephone or videoconferencing. A specific question was included regarding the impact of COVID-19 on their hopes and dreams for the future. Rapid identification of themes with an audio recordings technique was used to generate themes from the data.

Results

The 90 participants were predominantly female (77%) and ranged in age from 20 to 81 years with a mean age of 50 years and lived in several Australian states. Following immersive analysis of interviews, the identified common themes impacting people’s hopes and dreams revealed: concerns for their own and others’ job stability and future work; the impact on travel both for holidays, business and reconnecting with family; reassessing of personal and social values; and the intergenerational impact of such a profound pandemic, with concern for younger people particularly prominent in those concerns. Participants reflected on their loss of future dreams, with possibilities they had planned and worked towards not possible in the short term.

Conclusions

The responses provide a window into how people view their future goals and aspirations during a time of global and local instability and highlights the potential future impacts of the COVID-19 pandemic.
Association between social media use and the acceptance of COVID-19 vaccination among the general population in Saudi Arabia – a cross-sectional study
Authors: Othman Sahar S. , Alsuwaidi Abeer , Aseel Rafal , Alotaibi Reema , Bablgoom Reem , alsulami Ghadeer , Alharbi Razan , Ghamri Ranya
Published: February 2022

Background

The Coronavirus Disease of 2019 (COVID-19) pandemic is a worldwide global public health threat. Although acceptance of COVID-19 vaccination will be a critical step in combating the pandemic, achieving high uptake will be difficult, and potentially made more difficult by social media misinformation. This study aimed to examine the association between social media use and acceptance of receiving COVID-19 vaccine among the general population in Saudi Arabia.

Methodology

A cross-sectional study was conducted from June 17 to June 19, 2021 among 504 participants of the general population in Saudi Arabia. The data were collected using a three-part online questionnaire (sociodemographic characteristics, medical and vaccination history, pattern of social media use).

Results

Among 504 participants who completed the survey, 477 participants were acceptant of the vaccine and 27 were non-accepting. A total of 335 individuals had already received the vaccine, 142 were willing to receive the vaccine and 27 were unwilling. One hundred and thirty participants denied using social media for COVID-19 news. Four factors were found to be significant in influencing vaccine acceptance in univariate analysis: having a chronic condition (odds ratio (OR) = 0.367, P = 0.019), believing that infertility is a side effect of the COVID-19 vaccine (OR = 0.298, P = 0.009), being concerned about a serious side effect from the vaccine (somewhat concerned: OR = 0.294, P = 0.022, very concerned: OR = 0.017, P < 0.0001), and basing the decision to be vaccinated on social media information (OR = 0.260, P = 0.006). Two of these factors fell away on multivariate analysis: basing the decision on social media information (OR = 0.356, P = 0.071), and a belief that vaccination causes infertility (OR = 0.0333, P = 0.054), suggesting that the associations are dependent on other factors.

Conclusion

‏In conclusion, there was no significant independent relationship between social media usage and people's willingness to receive a COVID-19 vaccination. Further studies to explore the association between social media use and vaccine decisions are required to generalize this observation to the Saudi population.
Surveillance of physical activity and sedentary behaviour in czech children and adolescents: a scoping review of the literature from the past two decades
Authors: Materová Eliška , Pelclová Jana , Gába Aleš , Frömel Karel
Published: February 2022

Background

This study aimed to map the available evidence related to physical activity (PA) and sedentary behaviour (SB) in Czech children and adolescents and suggest future directions and improvements to strengthen the surveillance of PA and SB in the Czech Republic.

Methods

The search of articles published between January 2000 and December 2020 included the Medline and Medvik databases and a manual search in eight Czech journals related to the topic. This review followed the “Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews”.

Results

Out of 350 identified articles, 79 articles met the criteria for selection and referred to 27 studies. The majority of the articles were cross-sectional (89%), approximately two-thirds of the articles (61%) examined only PA, and half of the articles (51%) employed device-based assessments. Approximately 47% of the articles reported the prevalence of physical inactivity on the basis of inconsistently defined recommendations. Approximately 14%, 23%, and 10% of the articles focused on active transportation, organized PA (including physical education or leisure-time PA), and parent-child PA, respectively.

Conclusions

Future studies need to focus on longitudinal design and interventions, randomly selected samples, a mix of device-based and self-reported methods, and the recognition of health-related 24-hour time use patterns. This review advocates the government-supported development of a national surveillance system that will help to reduce insufficient PA and excessive SB.
Factors influencing follow-up care post-TIA and minor stroke: a qualitative study using the theoretical domains framework
Authors: Turner Grace M. , Aquino Maria Raisa Jessica V. , Atkins Lou , Foy Robbie , Mant Jonathan , Calvert Melanie
Published: February 2022

Background

Follow-up care after transient ischaemic attack (TIA) and minor stroke has been found to be sub-optimal, with individuals often feeling abandoned. We aimed to explore factors influencing holistic follow-up care after TIA and minor stroke.

Methods

Qualitative semi-structured interviews with 24 healthcare providers (HCPs): 5 stroke doctors, 4 nurses, 9 allied health professionals and 6 general practitioners. Participants were recruited from three TIA clinics, seven general practices and one community care trust in the West Midlands, England. Interview transcripts were deductively coded using the Theoretical Domains Framework and themes were generated from coded data.

Results

There was no clear pathway for supporting people with TIA or minor stroke after rapid specialist review in hospital; consequently, these patients had limited access to HCPs from all settings (‘Environmental context and resources’). There was lack of understanding of potential needs post-TIA/minor stroke, in particular residual problems such as anxiety/fatigue (‘Knowledge’). Identification and management of needs was largely influenced by HCPs’ perceived role, professional training (‘Social professional role and identity’) and time constraints (‘Environmental context and resources’). Follow-up was often passive – with onerous on patients to seek support – and predominantly focused on acute medical management (‘Intentions’/‘Goal’).

Conclusions

Follow-up care post-TIA/minor stroke is currently sub-optimal. Through identifying factors which influence follow-up, we can inform guidelines and practical strategies to improve holistic healthcare.
US local public health department spending between 2008 and 2016 did not increase for communities in need
Authors: Reszczynski Olivia , Connolly John , Shultz Kaitlyn , Kelly Sheila , Mitra Nandita , Hom Jeffrey , Venkataramani Atheendar , Chaiyachati Krisda H.
Published: February 2022

Background

Greater US local public health department (LPHD) spending has been associated with decreases in population-wide mortality. We examined the association between changes in LPHD spending between 2008 and 2016 and county-level sociodemographic indicators of public health need.

Methods

Multivariable linear regression was used to estimate the association between changes in county-level per-capita LPHD spending and 2008 sociodemographic indicators of interest: percent of population that was over 65 years old, Black, Hispanic, in poverty, unemployed, and uninsured. A second model assessed the relationship between changes in LPHD spending and sociodemographic shifts between 2008 and 2016.

Results

LPHD spending increases were associated with higher percentage points of 2008 adults over 65 years of age (+$0.53 per higher percentage point; 95% CI: +$0.01 to +$1.06) and unemployment (−$1.31; 95% CI: −$2.34 to −$0.27). Spending did not increase for communities with a higher proportion of people who identified as Black or Hispanic, or those with a greater proportion of people in poverty or uninsured, using either baseline or sociodemographic shifts between 2008 and 2016.

Conclusion

Future LPHD funding decisions should consider increasing investments in counties serving disadvantaged communities to counteract the social, political, and structural barriers which have historically prevented these communities from achieving better health.
Application of engineered extracellular vesicles for targeted tumor therapy
Authors: Zhang Fusheng , Guo Jinshuai , Zhang Zhenghou , Duan Meiqi , Wang Guang , Qian Yiping , Zhao Haiying , Yang Zhi , Jiang Xiaofeng
Published: February 2022

All cells, including prokaryotes and eukaryotes, could release extracellular vesicles (EVs). EVs contain many cellular components, including RNA, and surface proteins, and are essential for maintaining normal intercellular communication and homeostasis of the internal environment. EVs released from different tissues and cells exhibit excellent properties and functions (e.g., targeting specificity, regulatory ability, physical durability, and immunogenicity), rendering them a potential new option for drug delivery and precision therapy. EVs have been demonstrated to transport antitumor drugs for tumor therapy; additionally, EVs' contents and surface substance can be altered to improve their therapeutic efficacy in the clinic by boosting targeting potential and drug delivery effectiveness. EVs can regulate immune system function by affecting the tumor microenvironment, thereby inhibiting tumor progression. Co-delivery systems for EVs can be utilized to further improve the drug delivery efficiency of EVs, including hydrogels and liposomes. In this review, we discuss the isolation technologies of EVs, as well as engineering approaches to their modification. Moreover, we evaluate the therapeutic potential of EVs in tumors, including engineered extracellular vesicles and EVs' co-delivery systems.
Current applications and future perspective of CRISPR/Cas9 gene editing in cancer
Authors: Wang Si-Wei , Gao Chao , Zheng Yi-Min , Yi Li , Lu Jia-Cheng , Huang Xiao-Yong , Cai Jia-Bin , Zhang Peng-Fei , Cui Yue-Hong , Ke Ai-Wu
Published: February 2022

Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated nuclease 9 (CRISPR/Cas9) genome editing system. Due to its high efficiency and precision, the CRISPR/Cas9 technique has been employed to explore the functions of cancer-related genes, establish tumor-bearing animal models and probe drug targets, vastly increasing our understanding of cancer genomics. Here, we review current status of CRISPR/Cas9 gene editing technology in oncological research. We first explain the basic principles of CRISPR/Cas9 gene editing and introduce several new CRISPR-based gene editing modes. We next detail the rapid progress of CRISPR screening in revealing tumorigenesis, metastasis, and drug resistance mechanisms. In addition, we introduce CRISPR/Cas9 system delivery vectors and finally demonstrate the potential of CRISPR/Cas9 engineering to enhance the effect of adoptive T cell therapy (ACT) and reduce adverse reactions.
The VEGF expression associated with prognosis in patients with intrahepatic cholangiocarcinoma: a systematic review and meta-analysis
Authors: Cai Chunping , Wang Xiaoji , Fu Qiurong , Chen Ai
Published: February 2022

Objective

To systematically evaluate the relationship between vascular endothelial growth factor (VEGF) and prognosis of intrahepatic cholangiocarcinoma by meta-analysis.

Methods

We systematically searched relevant studies in the databases of PubMed, Embase, Cochrane Library, CNKI, Wangfang, and Web of Science, with search dates limited to September 1, 2021. We extracted relevant data, including prognosis and clinicopathological features of patients with different expressions of VEGF in intrahepatic cholangiocarcinoma. The combined hazard ratio (HR), odds ratio (OR), and 95% confidence interval (CI) were calculated to evaluate the link strength between VEGF and prognosis of cholangiocarcinoma patients.

Results

A total of 7 eligible studies with 495 patients were included in this meta-analysis. The results showed that the high expression of VEGF was significantly related to poor overall survival (OS) (HR = 1.93, 95% CI 1.52–2.46, P < 0.05) in patients with intrahepatic cholangiocarcinoma. Moreover, high expression of VEGF in tumor tissues associated with lymph node metastasis (LNM) (OR = 6.79, 95% CI 3.93–11.73, P < 0.05) and advanced TNM stage (OR = 4.35, 95% CI 2.34–8.07, P < 0.05) in intrahepatic cholangiocarcinoma. Sensitivity analysis shows that the meta-analysis results are stable and reliable.

Conclusion

The expression of VEGF is related to the OS of patients with intrahepatic cholangiocarcinoma, and the OS of patients with high expression of VEGF is shorter. VEGF may be a novel predictor of intrahepatic cholangiocarcinoma patients.

Trial registration

PROSPERO (CRD42022297443).
Proteome-defined changes in cellular pathways for decidua and trophoblast tissues associated with location and viability of early-stage pregnancy
Authors: Beer Lynn A. , Senapati Suneeta , Sammel Mary D. , Barnhart Kurt T. , Schreiber Courtney A. , Speicher David W.
Published: February 2022

Background

In early pregnancy, differentiating between a normal intrauterine pregnancy (IUP) and abnormal gestations including early pregnancy loss (EPL) or ectopic pregnancy (EP) is a major clinical challenge when ultrasound is not yet diagnostic. Clinical treatments for these outcomes are drastically different making early, accurate diagnosis imperative. Hence, a greater understanding of the biological mechanisms involved in these early pregnancy complications could lead to new molecular diagnostics.

Methods

Trophoblast and endometrial tissue was collected from consenting women having an IUP (n = 4), EPL (n = 4), or EP (n = 2). Samples were analyzed by LC–MS/MS followed by a label-free proteomics analysis in an exploratory study. For each tissue type, pairwise comparisons of different pregnancy outcomes (EPL vs. IUP and EP vs. IUP) were performed, and protein changes having a fold change ≥ 3 and a Student’s t-test p-value ≤ 0.05 were defined as significant. Pathway and network classification tools were used to group significantly changing proteins based on their functional similarities.

Results

A total of 4792 and 4757 proteins were identified in decidua and trophoblast proteomes. For decidua, 125 protein levels (2.6% of the proteome) were significantly different between EP and IUP, whereas EPL and IUP decidua were more similar with only 68 (1.4%) differences. For trophoblasts, there were 66 (1.4%) differences between EPL and IUP. However, the largest group of 344 differences (7.2%) was observed between EP and IUP trophoblasts. In both tissues, proteins associated with ECM remodeling, cell adhesion and metabolic pathways showed decreases in EP specimens compared with IUP and EPL. In trophoblasts, EP showed elevation of inflammatory and immune response pathways.

Conclusions

Overall, differences between an EP and IUP are greater than the changes observed when comparing ongoing IUP and nonviable intrauterine pregnancies (EPL) in both decidua and trophoblast proteomes. Furthermore, differences between EP and IUP were much higher in the trophoblast than in the decidua. This observation is true for the total number of protein changes as well as the extent of changes in upstream regulators and related pathways. This suggests that biomarkers and mechanisms of trophoblast function may be the best predictors of early pregnancy location and viability.
How mycobacterium tuberculosis infection could lead to the increasing risks of chronic fatigue syndrome and the potential immunological effects: a population-based retrospective cohort study
Authors: Yang Tse-Yen , Lin Cheng-Li , Yao Wei-Cheng , Lio Chon-Fu , Chiang Wen-Po , Lin Kuan , Kuo Chien-Feng , Tsai Shin-Yi
Published: February 2022

Background

Chronic fatigue syndrome (CFS) has been shown to be associated with infections. Tuberculosis (TB) is a highly prevalent infectious disease. Patients with chronic fatigue syndrome and post-tuberculosis experience similar symptoms. Furthermore, chronic fatigue syndrome and tuberculosis share similar plasma immunosignatures. This study aimed to clarify the risk of chronic fatigue syndrome following the diagnosis of Mycobacterium tuberculosis infection (MTI), by analyzing the National Health Insurance Research Database of Taiwan.

Methods

7666 patients aged 20 years or older with newly diagnosed Mycobacterium tuberculosis infection during 2000–2011 and 30,663 participants without Mycobacterium tuberculosis infection were identified. Both groups were followed up until the diagnoses of chronic fatigue syndrome were made at the end of 2011.

Results

The relationship between Mycobacterium tuberculosis infection and the subsequent risk of chronic fatigue syndrome was estimated through Cox proportional hazards regression analysis, with the incidence density rates being 3.04 and 3.69 per 1000 person‐years among the non‐Mycobacterium tuberculosis infection and Mycobacterium tuberculosis infection populations, respectively (adjusted hazard ratio [HR] = 1.23, with 95% confidence interval [CI] 1.03–1.47). In the stratified analysis, the Mycobacterium tuberculosis infection group were consistently associated with a higher risk of chronic fatigue syndrome in the male sex (HR = 1.27, 95% CI 1.02–1.58) and age group of ≥ 65 years old (HR = 2.50, 95% CI 1.86–3.38).

Conclusions

The data from this population‐based retrospective cohort study revealed that Mycobacterium tuberculosis infection is associated with an elevated risk of subsequent chronic fatigue syndrome.
Rare isolation of human-tropic recombinant porcine endogenous retroviruses PERV-A/C from Göttingen minipigs
Authors: Halecker Sabrina , Krabben Ludwig , Kristiansen Yannick , Krüger Luise , Möller Lars , Becher Dietmar , Laue Michael , Kaufer Benedikt , Reimer Christian , Denner Joachim
Published: February 2022

Background

Porcine endogenous retroviruses (PERVs) can infect human cells and pose a risk for xenotransplantation when pig cells, tissues or organs are transplanted to human recipients. Xenotransplantation holds great promise to overcome the shortage of human donor organs after solving the problems of rejection, functionality and virus safety. We recently described the transmission of a human-tropic recombinant PERV-A/C, designated PERV-F, from peripheral blood mononuclear cells (PBMCs) of a Göttingen Minipig (GöMP) to human 293 cells (Krüger et al., in Viruses 12(1):38, 2019). The goal of this study was to characterize PERV-F in more detail and to analyze the probability of virus isolation from other animals.

Methods

The recombination site in the envelope (env) gene, the long terminal repeats (LTR), the proteins and the morphology of the recombinant PERV-F were characterized by polymerase chain reaction (PCR), sequencing, Western blot analysis, immunofluorescence, and transmissible electron microscopy. Mitogen-stimulated PBMCs from 47 additional pigs, including 17 new GöMP, were co-cultured with highly susceptible human 293 T cells, and the PERV-A/C prevalence and PERV transmission was analyzed by PCR.

Results

PERV-F, isolated from a GöMP, is an infectious human-tropic PERV-A/C virus with a novel type of recombination in theenvgene. The length of the LTR of PERV-F increased after passaging on human cells. In a few minipigs, but not in German landrace pigs, PERV-A/C were found. There was no transmission of human-tropic PERV-A/C from additional 47 pigs, including 17 GöMP, to human cells.

Conclusion

These data show that human-tropic recombinant PERV-A/C proviruses can only be found in a very small number of minipigs, but not in other pigs, and that their isolation as infectious virus able to replicate on human cells is an extremely rare event, even when using highly susceptible 293 cells.
The pro-inflammatory effects of combined exposure to diesel exhaust particles and mineral particles in human bronchial epithelial cells
Authors: Grytting Vegard Sæter , Chand Prem , Låg Marit , Øvrevik Johan , Refsnes Magne
Published: February 2022

Background

People are exposed to ambient particulate matter (PM) from multiple sources simultaneously in both environmental and occupational settings. However, combinatory effects of particles from different sources have received little attention in experimental studies. In the present study, the pro-inflammatory effects of combined exposure to diesel exhaust particles (DEP) and mineral particles, two common PM constituents, were explored in human lung epithelial cells.

Methods

Particle-induced secretion of pro-inflammatory cytokines (CXCL8 and IL-1β) and changes in expression of genes related to inflammation (CXCL8, IL-1α, IL-1β and COX-2), redox responses (HO-1) and xenobiotic metabolism (CYP1A1 and CYP1B1) were assessed in human bronchial epithelial cells (HBEC3-KT) after combined exposure to different samples of DEP and mineral particles. Combined exposure was also conducted using lipophilic organic extracts of DEP to assess the contribution of soluble organic chemicals. Moreover, the role of the aryl hydrocarbon receptor (AhR) pathway was assessed using an AhR-specific inhibitor (CH223191).

Results

Combined exposure to DEP and mineral particles induced increases in pro-inflammatory cytokines and expression of genes related to inflammation and redox responses in HBEC3-KT cells that were greater than either particle sample alone. Moreover, robust increases in the expression of CYP1A1 and CYP1B1 were observed. The effects were most pronounced after combined exposure to α-quartz and DEP from an older fossil diesel, but enhanced responses were also observed using DEP generated from a modern biodiesel blend and several stone particle samples of mixed mineral composition. Moreover, the effect of combined exposure on cytokine secretion could also be induced by lipophilic organic extracts of DEP. Pre-incubation with an AhR-specific inhibitor reduced the particle-induced cytokine responses, suggesting that the effects were at least partially dependent on AhR.

Conclusions

Exposure to DEP and mineral particles in combination induces enhanced pro-inflammatory responses in human bronchial epithelial cells compared with exposure to the individual particle samples. The effects are partly mediated through an AhR-dependent pathway and lipophilic organic chemicals in DEP appear to play a central role. These possible combinatory effects between different sources and components of PM warrant further attention and should also be considered when assessing measures to reduce PM-induced health effects.
Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry
Authors: Wieczorek Dorota , Żyszka-Haberecht Beata , Kafka Anna , Lipok Jacek
Published: February 2022

Although the spectrum of effective methods and techniques that allow determination of inorganic or total phosphorus is impressive, more precise analysis of these substances in plant tissues is not a routine or trivial task. The complexity of chemical composition of plant tissues treated as the analytical matrices is thought to be the main cause why there is no one answer, how appropriate phosphorus compounds may be determined qualitatively and quantitatively. Even if more advanced spectrophotometric measurements and classical variants of absorption (FAAS) or emission (ICP-AES/ ICP-OES) spectrometry techniques are used, it is necessary at first to isolate various forms of phosphorus from the matrix, and then to mineralize them prior the determination. Significant progress in such a kind of analytical efforts was brought by implementation of combined methods e.g. ETV-ICP-AES or HR-ETAAS, does allow the isolation of the phosphorus analyte and its detection during a kind of “one step” analytical procedure, directly in plant tissues. Similar benefits, regarding sensitivity of determinations, are obtained when XRF, SIMS or nanoSIMS—more expensive techniques of imaging the presence of phosphorus in biological matrices have been used. Nowadays, obviously being aware of higher limit of detection, nuclear magnetic resonance spectroscopy, especially the 31P NMR technique, is thought to be the most universal analytical tool allowing to determine various chemical forms of plant phosphorus qualitatively and quantitatively, at the same time. Although 31P NMR provides valuable information about the phosphorus profile of plants, it should be emphasized that each analytical issue related to the determination of phosphorus compounds in plant tissues and organs, requires an individual approach to defined problem.
Barriers, frameworks, and mitigating strategies influencing the dissemination and implementation of health promotion interventions in indigenous communities: a scoping review
Authors: Sacca Lea , Shegog Ross , Hernandez Belinda , Peskin Melissa , Rushing Stephanie Craig , Jessen Cornelia , Lane Travis , Markham Christine
Published: February 2022

Background

Many Indigenous communities across the USA and Canada experience a disproportionate burden of health disparities. Effective programs and interventions are essential to build protective skills for different age groups to improve health outcomes. Understanding the relevant barriers and facilitators to the successful dissemination, implementation, and retention of evidence-based interventions and/or evidence-informed programs in Indigenous communities can help guide their dissemination.

Purpose

To identify common barriers to dissemination and implementation (D&I) and effective mitigating frameworks and strategies used to successfully disseminate and implement evidence-based interventions and/or evidence-informed programs in American Indian/Alaska Native (AI/AN), Native Hawaiian/Pacific Islander (NH/PI), and Canadian Indigenous communities.

Methods

A scoping review, informed by the York methodology, comprised five steps: (1) identification of the research questions; (2) searching for relevant studies; (3) selection of studies relevant to the research questions; (4) data charting; and (5) collation, summarization, and reporting of results. The established D&I SISTER strategy taxonomy provided criteria for categorizing reported strategies.

Results

Candidate studies that met inclusion/exclusion criteria were extracted from PubMed (n= 19), Embase (n= 18), and Scopus (n= 1). Seventeen studies were excluded following full review resulting in 21 included studies. The most frequently cited category of barriers was “Social Determinants of Health in Communities.” Forty-three percent of barriers were categorized in this community/society-policy level of the SEM and most studies (n= 12, 57%) cited this category. Sixteen studies (76%) used a D&I framework or model (mainly CBPR) to disseminate and implement health promotion evidence-based programs in Indigenous communities. Most highly ranked strategies (80%) corresponded with those previously identified as “important” and “feasible” for D&I The most commonly reported SISTER strategy was “Build partnerships (i.e., coalitions) to support implementation” (86%).

Conclusion

D&I frameworks and strategies are increasingly cited as informing the adoption, implementation, and sustainability of evidence-based programs within Indigenous communities. This study contributes towards identifying barriers and effective D&I frameworks and strategies critical to improving reach and sustainability of evidence-based programs in Indigenous communities.

Registration number

N/A (scoping review)
Effect of tourniquet use on blood loss, pain, functional recovery, and complications in robot-assisted total knee arthroplasty: a prospective, double-blinded, randomized controlled trial
Authors: Lai Ya-hao , Xu Hong , Su Qiang , Wan Xu-feng , Yuan Ming-cheng , Zhou Zong-ke
Published: February 2022

Background

Robot-assisted total knee arthroplasty (TKA) has been largely studied to confirm its advantages in terms of accurate component positioning, microembolus formation, less blood loss, and so on, but is currently usually performed under tourniquet due to its longer operative time than conventional TKA. The aim of this study was to estimate the effects of tourniquet use in robot-assisted TKA on blood loss, pain, functional recovery, and complications.

Methods

Patients scheduled for robot-assisted TKA were prospectively randomized into a tourniquet or non-tourniquet group (each n = 14). The primary outcome measure was blood loss. The secondary outcome measures were operation time; visual analog scale (VAS) pain scores; time to achieve the first straight-leg raise; swelling of the thigh, knee, and calf; range of motion; Hospital for Special Surgery score; length of stay; and postoperative complications.

Results

There was no significant difference in total blood loss between the tourniquet and non-tourniquet groups (738.57 ± 276.158 vs. 866.85 ± 243.422 ml, P = 0.061). The tourniquet group showed significantly lower intraoperative blood loss (P < 0.001), but higher hidden blood loss (P = 0.002). The non-tourniquet group showed better knee range of motion on postoperative days (PODs) 1–3 (all P < 0.001), less thigh swelling on PODs 2 and 3 (P < 0.05), earlier straight-leg raising (P = 0.044), and shorter length of stay (P = 0.044). Thigh pain VAS score at 1 month after surgery was significantly greater in the tourniquet group (P < 0.001), as was knee pain during activity and at rest on PODs 2–3 (all P < 0.05). The tourniquet group also showed a significantly higher rate of tension blisters (28.8% vs. 7.1%, P = 0.038).

Conclusions

Tourniquet use during robot-assisted TKA does not reduce total blood loss, and it appears to increase postoperative pain, aggravate muscle injury, and prolong postoperative recovery.

Trial registration ChiCTR, ChiCTR2100041800. Registered 5 January 2021, http://www.chictr.org.cn/index.aspx.
Contamination detection in genomic data: more is not enough
Authors: Cornet Luc , Baurain Denis
Published: February 2022

The decreasing cost of sequencing and concomitant augmentation of publicly available genomes have created an acute need for automated software to assess genomic contamination. During the last 6 years, 18 programs have been published, each with its own strengths and weaknesses. Deciding which tools to use becomes more and more difficult without an understanding of the underlying algorithms. We review these programs, benchmarking six of them, and present their main operating principles. This article is intended to guide researchers in the selection of appropriate tools for specific applications. Finally, we present future challenges in the developing field of contamination detection.
The Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel
Authors: Fitzgerald Tomas , Brettell Ian , Leger Adrien , Wolf Nadeshda , Kusminski Natalja , Monahan Jack , Barton Carl , Herder Cathrin , Aadepu Narendar , Gierten Jakob , Becker Clara , Hammouda Omar T. , Hasel Eva , Lischik Colin , Lust Katharina , Sokolova Natalia , Suzuki Risa , Tsingos Erika , Tavhelidse Tinatini , Thumberger Thomas , Watson Philip , Welz Bettina , Khouja Nadia , Naruse Kiyoshi , Birney Ewan , Wittbrodt Joachim , Loosli Felix
Published: February 2022

Background

Unraveling the relationship between genetic variation and phenotypic traits remains a fundamental challenge in biology. Mapping variants underlying complex traits while controlling for confounding environmental factors is often problematic. To address this, we establish a vertebrate genetic resource specifically to allow for robust genotype-to-phenotype investigations. The teleost medaka (Oryzias latipes) is an established genetic model system with a long history of genetic research and a high tolerance to inbreeding from the wild.

Results

Here we present the Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel: the first near-isogenic panel of 80 inbred lines in a vertebrate model derived from a wild founder population. Inbred lines provide fixed genomes that are a prerequisite for the replication of studies, studies which vary both the genetics and environment in a controlled manner, and functional testing. The MIKK panel will therefore enable phenotype-to-genotype association studies of complex genetic traits while allowing for careful control of interacting factors, with numerous applications in genetic research, human health, drug development, and fundamental biology.

Conclusions

Here we present a detailed characterization of the genetic variation across the MIKK panel, which provides a rich and unique genetic resource to the community by enabling large-scale experiments for mapping complex traits.
Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues
Authors: Lakew Aynalem , Assefa Teshome , Woldeyohannes Meseret , Megersa Negussie , Chandravanshi Bhagwan Singh
Published: February 2022

Background

Antibiotics are routinely used on poultry for therapy and prevention of diseases and to enhance animal growth. The objective of this study was to develop and validate a sensitive and reliable liquid chromatography with UV detection (LC-UV) method for the simultaneous determination of seven multiclass antibiotic residues (amoxicillin, ampicillin, penicillin, sulfamethoxazole, gentamicin, ciprofloxacin, and erythromycin) in chicken tissues.

Methods

The liquid chromatography method with UV detection was optimized for complete separation of the seven selected antibiotic compounds with reversed phase and isocratic elution using Hypersil BDS-C18 (3 µm, 100 mm × 4 mm) column. The mobile phase consisted a ratio of 0.05 M Na2HPO4, acetonitrile and methanol (70:10:20), at UV absorption wavelength of 230 nm. The column thermostat was set at 40 °C, the mobile phase flow rate was 1 mL min−1, and the injection volume was 20 μL.

Results

All the seven standard compounds were eluted within 14 min. The results for: linearity, precision, sensitivity, accuracy, specificity, decision limit (CCα), detection capability (CCβ), suitability and method robustness were validated according to the criteria of Commission Decision 2002/657/EC guidelines. Calibration plot correlation coefficients ranged from 0.9983 to 0.9998 and the percent relative standard deviations for repeated analysis were below 5% indicating acceptable method precision. The limits of detection (LODs) and quantification (LOQs) ranged from 0.098–0.255 μg kg−1 to 0.297–0.574 μg kg−1, respectively. The accuracy study yielded recoveries in the ranges 98.1–107% for the pure compounds and 94.0–102% for the spiked drug free chicken tissue samples.

Conclusions

The method was found to be appropriate for simultaneous determination of five different classes of seven antibiotic residues in chicken tissues. Furthermore, this is the first instance for the simultaneous determination of seven multiclass, multi-residues analysis using LC-UV from chicken tissue samples. This is a cost-effective and alternative method with simple instrumentation approach for laboratories that lack highly specialized state-of-the-art instrumentation.
Between two stools: preclinical research, reproducibility, and statistical design of experiments
Authors: Reynolds Penny S.
Published: February 2022

Translation of animal-based preclinical research is hampered by poor validity and reproducibility issues. Unfortunately, preclinical research has ‘fallen between the stools’ of competing study design traditions. Preclinical studies are often characterised by small sample sizes, large variability, and ‘problem’ data. Although Fisher-type designs with randomisation and blocking are appropriate and have been vigorously promoted, structured statistically-based designs are almost unknown. Traditional analysis methods are commonly misapplied, and basic terminology and principles of inference testing misinterpreted. Problems are compounded by the lack of adequate statistical training for researchers, and failure of statistical educators to account for the unique demands of preclinical research. The solution is a return to the basics: statistical education tailored to non-statistician investigators, with clear communication of statistical concepts, and curricula that address design and data issues specific to preclinical research. Statistics curricula should focus on statistics as process: data sampling and study design before analysis and inference. Properly-designed and analysed experiments are a matter of ethics as much as procedure. Shifting the focus of statistical education from rote hypothesis testing to sound methodology will reduce the numbers of animals wasted in noninformative experiments and increase overall scientific quality and value of published research.
Nanoliposome-encapsulated phenolic-rich fraction from Alcea rosea as a dietary phytobiotic in mice challenged by Escherichia coli
Authors: Hassirian Niloofar , Karimi Ehsan , Oskoueian Ehsan
Published: February 2022

Purpose

This research was performed to evaluate the antibacterial and health-promoting potentials of nanoliposome-encapsulated phenolic-rich fraction (PRF) from Alcea rosea leaves, as a dietary phytobiotic, in mice as challenged by enteropathogenic Escherichia coli (E. coli; O157: H7).

Method

The PEF was encapsulated in nanoliposomes (PEF-NLs), and the phenolic profiling of PEF-NLs was confirmed by HPLC. Next, 40 white male balb/c mice were assigned to four treatment groups to assess the antibacterial potential of PEF-NLs by measuring the blood parameters and the liver’s lipid peroxidation in the mice as a result of the infection caused by E. coli. Finally, the expression of cyclooxygenase 2 (COX2), inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were determined in the miceʼs ileum tissues. A real-time PCR was used to analyze the relative fold changes in the population of E. coli in the ileum.

Results

The overall results demonstrated that the nanoliposome-loaded PRF contained gallic acid, salicylic acid, pyrogallol, cinnamic acid, catechin, naringin, and ferulic acid. The E. coli intervention impaired the mice's weight gain, food intake, liver enzymes, lipid peroxidation, and the ileum’s morphometric characteristics. The challenge also upregulated the inflammatory genes (COX2, iNOS), downregulated the antioxidant-related genes (SOD and GPx), and increased the population of E. coli in the ileum. The dietary inclusion of the nonencapsulated PRF and the nanoliposome-encapsulated PRF, at the concentration of 10 mg TPC/kg BW/day, improved these parameters. However, compared to nonencapsulated PRF, the nanoliposome-encapsulated PRF appeared to be more effective in improving the health parameters in mice.

Conclusion

As a promising phytobiotic, the nanoliposome-encapsulated PRF could play a critical role against the E. coli infection in mice probably due to the increase in the higher intestinal solubility, bioavailability, and absorption of phenolic compounds encapsulated in the nanoliposome carrier.
Optimization of the energy efficiency in Smart Internet of Vehicles assisted by MEC
Authors: Fu Jiafei , Zhu Pengcheng , Hua Jingyu , Li Jiamin , Wen Jiangang
Published: February 2022

Smart Internet of Vehicles (IoV) as a promising application in Internet of Things emerges with the development of the fifth generation mobile communication (5G). Nevertheless, the heterogeneous requirements of sufficient battery capacity, powerful computing ability and energy efficiency for electric vehicles face great challenges due to the explosive data growth in 5G and the sixth generation of mobile communication (6G) networks. In order to alleviate the deficiencies mentioned above, this paper proposes a mobile edge computing (MEC) enabled IoV system, in which electric vehicle nodes upload and download data through an anchor node which is integrated with a MEC server. Meanwhile, the anchor node transmitters radio signal to electric vehicles with simultaneous wireless information and power transfer technology so as to compensate the battery limitation of eletric vehicles. Moreover, the spectrum efficiency is further improved by multi-input and multi-output and full-duplex technologies which is equipped at the anchor node. In consideration of the issues above, we maximize the average energy efficiency of electric vehicles by jointly optimize the CPU frequency, vehicle transmitting power, computing tasks and uplink rate. Since the problem is nonconvex, we propose a novel alternate interior-point iterative scheme (AIIS) under the constraints of computing tasks, energy consumption and time latency. Results and discussion section verifies the effectiveness of the proposed AIIS scheme comparing with the benchmark schemes.
Influence of occupant presence patterns on energy consumption and its relation to comfort: a case study based on sensor and crowd-sensed data
Authors: Rusek Robert , Melendez Frigola Joaquim , Colomer Llinas Joan
Published: February 2022

Background

In recent years, the monitoring of occupant presence patterns has become an imperative for building energy optimization. Very often, there is a significant discrepancy between the building energy performance predicted at the design stage and the actual performance rendered during the building operation. This stems from the difference in user occupancy. In spite of this, user interaction and feedback are rarely taken into account and evidence of the impact of occupant presence patterns on energy consumption is still scarce. Thus, the purpose of this study is to apply crowd-sensing techniques to understand how energy is consumed and how appropriate performance indicators should be defined to provide inputs for building operations regarding more efficient use of resources.

Methods

Monitoring strategies were implemented in an office lab with controlled variables to collect quantitative data on occupancy patterns, ambient factors and energy consumption. In addition, crowd-sensing techniques were applied to model user activity in different ambient conditions over time and to contrast their occupancy with energy consumption patterns in combination with new inquiry tools to identify how occupants perceive their comfort level. In addition, a set of energy efficiency indicators was used to compare energy performance over different periods.

Results

It was discovered that there is a strong relation between user occupancy patterns and energy consumption. However, more than 50% of energy was consumed when no user activity was registered. Energy performance indicators revealed that measuring energy efficiency in terms of kWh per surface area encourages a less efficient use of space and, therefore, including a coefficient of person hours is advisable. It was also discovered that users do not fully rely on feedback mechanisms and they prefer to take action to adapt the ambient conditions rather than simply expressing their opinion. Analysis of energy usage during the Covid-19 lock down revealed substantial use of energy contrary to what was expected. This was because home computers were used as terminals only, while the actual tasks were performed on the lab computers, using remote desktop connections, which were turned on 24/7. In addition, energy consumed by each employee at his/her home should be taken into account. Moreover, a set of practical recommendations was formulated.
Incidence and risk factors of surgical site infections and related antibiotic resistance in Freetown, Sierra Leone: a prospective cohort study
Authors: Lakoh Sulaiman , Yi Le , Sevalie Stephen , Guo Xuejun , Adekanmbi Olukemi , Smalle Isaac O. , Williams Nathaniel , Barrie Umu , Koroma Celesis , Zhao Yongkun , Kamara Matilda N. , Cummings-John Constance , Jiba Darlinda F. , Namanaga Enanga Sonia , Deen Betsy , Zhang Juling , Maruta Anna , Kallon Christiana , Liu Peng , Wurie Haja Ramatulai , Kanu Joseph Sam , Deen Gibrilla F. , Samai Mohamed , Sahr Foday , Firima Emmanuel
Published: February 2022

Background

There is limited information on surgical site infections (SSI) and the related antibiotic resistance needed to guide their management and prevention in Sierra Leone. In this study, we aimed to establish the incidence and risk factors of SSI and the related antibiotic resistance among adults attending a tertiary hospital, and a secondary health facility in Freetown, Sierra Leone.

Methods

This is a prospective cohort study designed to collect data from adult (18 years or older) patients who attended elective and emergency surgeries at two hospitals in Freetown between February and July, 2021. Data analysis was done using STATA version 16.

Results

Of 338 patients, 245 (72.5%) and 93 (27.5%) had their surgeries at the tertiary and secondary hospitals, respectively. Many were males 192 (56.8%), less than 35 years 164 (48.5%), and 39 (11.5%) developed an SSI. Of the 39 patients who acquired an SSI, 7 (17.9%) and 32 (82.1%) had their surgeries at the secondary and tertiary hospitals, respectively. The incidence of SSI is higher in contaminated 17 (43.6%) than in clean-contaminated 12 (30.8%) and clean 10 (25.6%) wounds. Wound swabs were collected in 29 (74.4%) patients, of which 18 (62.1%) had bacterial growth. In total, 49 isolates of 14 different bacteria including gram-negative 41 (83.7%) and gram-positive 8 (16.3%) isolates were identified. Of these, 32 (65.3%) were Enterobacteriaceae, 9 (18.4%) were Non-fermenting gram-negative bacilli and 10 (12.2%) were Enterococci. The most common isolates were Escherichia coli (12, 24.5%), Klebsiella pneumoniae (10, 20.4%), Acinetobacter baumannii (5, 10.2%), Klebsiella oxytoca (4, 8.2%) and Enterococcus faecalis (4, 8.2%). The Enterobacteriaceae were either resistance to carbapenems (4, 8.2%) or were extended-spectrum beta-lactamase (ESBL) producing organisms (29, 59.2%). Male sex [p = 0.031], an ASA score ≥ 2 [p = 0.020), administration of general anaesthesia [p = 0.018] and elevated fasting glucose [p = 0.033] were predictive of SSI.

Conclusion

The incidence of SSI in this study is comparable to other low- and middle-income countries, but a substantial proportion of these postoperative wounds have an ESBL-producing Enterobacteriaceae. Therefore, routine surveillance of SSI and related antibiotic resistance is required in resource-limited settings.