bluuebunny/crossref_metadata_2025 huggingface dataset:
Aberrant Transcript Usage Is Associated with Homologous Recombination Deficiency and Predicts Therapeutic Response
Authors: Kang Hyeon Gu , Hwangbo Haeun , Kim Myung Ji , Kim Sinae , Lee Eun Ji , Park Min Ji , Kim Jae-Weon , Kim Byoung-Gie , Cho Eun-Hae , Chang Suhwan , Lee Jung-Yun , Choi Jung Kyoon
Published: October 2021

BRCA1/2 mutations account for only a small fraction of homologous recombination (HR) deficiency (HRD) cases. Recently developed genomic HRD (gHRD) tests suffer confounding factors that cause low precision in predicting samples that will respond to PARP inhibitors and DNA damaging agents. Here we present molecular and clinical evidence of transcriptional HRD (tHRD) that is based on aberrant transcript usage (aTU) of minor isoforms. Specifically, increased TU of nonfunctional isoforms of DNA repair genes was prevalent in breast and ovarian cancer with gHRD. Functional assays validated the association of aTU with impaired HR activity. Machine learning–based tHRD detection by the transcript usage (TU) pattern of key genes was superior to directly screening for gHRD or BRCA1/2 mutations in accurately predicting responses of cell lines and patients with cancer to PARP inhibitors and genotoxic drugs. This approach demonstrated the capability of tHRD status to reflect functional HR status, including in a cohort of olaparib-treated ovarian cancer with acquired platinum resistance. Diagnostic tests based on tHRD are expected to broaden the clinical utility of PARP inhibitors.

Significance:

A novel but widespread transcriptional mechanism by which homologous recombination deficiency arises independently of BRCA1/2 mutations can be utilized as a companion diagnostic for PARP inhibitors.
U.S. FDA Drug Approvals for Gynecological Malignancies: A Decade in Review
Authors: Arora Shaily , Narayan Preeti , Ison Gwynn , Berman Tara , Suzman Daniel L. , Wedam Suparna , Prowell Tatiana M. , Ghosh Soma , Philip Reena , Osgood Christy L. , Gao Jennifer J. , Shah Mirat , Krol Danielle , Wahby Sakar , Royce Melanie , Brus Christina , Bloomquist Erik W. , Fiero Mallorie H. , Tang Shenghui , Pazdur Richard , Ibrahim Amna , Amiri-Kordestani Laleh , Beaver Julia A.
Published: October 2021

Over the last decade, there has been tremendous progress in the treatment of patients with gynecologic cancers with a changing therapy landscape. This summary provides an overview of U.S. Food and Drug Administration (FDA) approvals for gynecologic cancers from 2010 to 2020, totaling 17 new indications. For each of the approved indications, endpoints, trial design, results, and regulatory considerations are outlined. Among these 17 indications, six received accelerated approval (AA) and 11 received regular approval (RA). As of September 2021, of the six AA, three have subsequently demonstrated clinical benefit resulting in conversion to RA and the remaining three have ongoing clinical trials that have not yet reported results. Approval decisions for these 17 indications were supported by primary efficacy endpoints of progression-free survival (n = 10), objective response rate (n = 6), and overall survival (n = 1) and showed a favorable benefit–risk profile. Among the 17 indications, 15 received priority review and three applications participated in one or more novel Oncology Center of Excellence initiatives, including Real Time Oncology Review, Assessment Aid, and Project Orbis. Current FDA thinking on drug development opportunities and regulatory initiatives currently under way will be discussed.
U.S. FDA Drug Approvals for Breast Cancer: A Decade in Review
Authors: Arora Shaily , Narayan Preeti , Osgood Christy L. , Wedam Suparna , Prowell Tatiana M. , Gao Jennifer J. , Shah Mirat , Krol Danielle , Wahby Sakar , Royce Melanie , Ghosh Soma , Philip Reena , Ison Gwynn , Berman Tara , Brus Christina , Bloomquist Erik W. , Fiero Mallorie H. , Tang Shenghui , Pazdur Richard , Ibrahim Amna , Amiri-Kordestani Laleh , Beaver Julia A.
Published: October 2021

Over the last decade, the treatment of patients with breast cancer has been greatly impacted by the approval of multiple drugs and indications. This summary describes 30 FDA approvals of treatments for breast cancer from 2010 to 2020. The trial design endpoints, results, and regulatory considerations are described for each approved indication. Of the 30 indications, 23 (76.6%) received regular and 7 (23.3%) received accelerated approval. Twenty-six approvals were granted in metastatic breast cancer (MBC) and four in early breast cancer. Approval decisions for the 26 MBC indications were initially supported by progression-free survival (PFS) in 21 (80.8%), overall survival (OS) or a combination of OS and PFS in two (7.7%), and objective response rate (ORR) in three (11.5%). The four approvals in early breast cancer utilized pathologic complete response (pCR) in one (25%) and invasive disease-free survival (iDFS) in three (75%) trials. Among the 30 indications, 22 received priority review, seven were granted Breakthrough Therapy Designation, and 10 applications participated in one or more pilot Oncology Center of Excellence regulatory review initiatives, including Real Time Oncology Review, Assessment Aid, and Project Orbis. FDA initiatives to advance breast cancer drug development are also described.
Positive Anti-GABAB Receptor Antibodies in a Patient with Hashimoto’s Thyroiditis and Bipolar Affective Disorder
Authors: Sobolevskaia Polina A. , Andreev Boris V. , Churilov Leonid P. , Fedotkina Tamara V. , Gilburd Boris , Stanova Aliya K. , Utekhin Vladimir J. , Shoenfeld Yehuda
Published: October 2021

A kind of autoimmune encephalitis with positive autoantibodies toward the B1 subunit of the gamma-aminobutyric acid-type B receptor is known as anti-GABABR encephalitis. It is an autoimmune brain disorder with typical manifestations of a limbic encephalitis. It can coexist with positive anti-thyroid autoantibodies. We described a 57-year-old woman who was diagnosed with a bipolar affective disorder. The patient had high titers of anti-thyroid peroxidase antibodies, and she also was positive for anti-GABABR antibodies. There is a question, if this case is a kind of comorbidity of anti-thyroid and anti-brain autoimmunity or is it a single nosological entity – a kind of Hashimoto’s encephalopathy?
Clio e Psique: limites e perspectivas de diálogo
Authors: Franco Júnior Hilário
Published: October 2021

Entre a história e a psicanálise seria de se esperar colaboração sistemática e constante, que não acontece devido a divergências próprias ao perfil de cada uma das áreas e a certa concorrência no interior do universo científico. Contudo, elas apresentam afinidades quanto ao propósito (compreensão do ser humano, social e individual), ao material utilizado (fragmentos do passado observado), ao método adotado (indutivo). Assim, o melhor conhecimento recíproco, proposto por este artigo, pode viabilizar a cooperação potencialmente favorável a ambas as disciplinas.
Knee subchondroplasty for management of subchondral bone cysts: a novel treatment method
Authors: Zeng GJ , Foong FS , Lie DTT
Published: October 2021

Knee subchondroplasty (SCP) is one of the most novel minimally invasive methods for treating bone marrow lesions. The literature suggests that it is safe, with few complications and good outcomes. However, no studies have documented its usage for managing large subchondral bone cysts. This article outlines a case report and details the pearls and pitfalls of SCP in treating large subchondral bone cysts. Our patient underwent arthroscopic debridement with medial femoral condyle SCP. Mild posterior extravasation of synthetic bone substitute was observed on Postoperative Day 1, which was immediately rectified on revision arthroscopy. Gradual escalation of weight bearing and good pain relief were subsequently achieved, and the patient has remained complication-free after two years. No further extravasation were observed on repeat radiography. SCP is a feasible temporising measure that may help to delay the need for bone allograft or immediate knee arthroplasty in younger patients while retaining function and delaying loss of productivity.
A new species of Galerina (Hymenogastraceae, Agaricales) from northeast China
Authors: LIU XIAOLIANG , BAU TOLGOR
Published: October 2021

Galerina variibasidia, a new species in the Hymenogastraceae, was discovered in Arxan, Inner Mongolia Autonomous Region, China. This species is presented with morphological description, illustrations, and supported by phylogenetic analyses using Maximum Likelihood and Bayesian Inference analyses with the internal transcribed spacer rDNA sequences.
New combinations in Hyacinthaceae subfam. Ornithogaloideae
Authors: MARTÍNEZ-AZORÍN MARIO , DOLD ANTHONY P. , CRESPO MANUEL B. , ALONSO-VARGAS MARÍA Á.
Published: October 2021

Within the framework of a taxonomic revision of Hyacinthaceae subfamily Ornithogaloideae, we present two new combinations in Eliokarmos for South African species, and five new combinations in Loncomelos for taxa occurring in Iran and Iraq. Details on the concerned types are given, and a new lectotype is designated.
A new lectotype for Isolepis levynsiana (Cyperaceae)
Authors: MUASYA A. MUTHAMA , SIMPSON DAVID A.
Published: October 2021

N/A
Tachinid flies (Diptera: Tachinidae) reared from deciduous plant-feeding lepidopteran larvae at Hokkaido University Tomakomai Forest (Japan), with descriptions of three new species
Authors: SHIMA HIROSHI , ABE TOMOKAZU , LIBRA MARTIN
Published: October 2021

Seven species of tachinid flies reared from herbivorous lepidopterans collected from the deciduous forest canopy at Hokkaido University Tomakomai Experimental Forest (0.2 ha plot), Hokkaido, Japan, are recorded and described: Blepharomyia brevicornis sp. nov. ex Erannis golda Djakonov (Geometridae), Catocala lara Bremer and Orthosia odiosa (Butler) (both Noctuidae); Ctenophorinia grisea Mesnil ex Himeropteryx miraculosa Staudinger (Notodontidae); Blepharipa carbonata (Mesnil) ex Marumba jankowskii (Oberthür) (Sphingidae); Cyzenis equifacialis sp. nov. ex Faristenia geminisignella Ponomarenko (Gelechiidae), Archips crataegana (Hübner), A. nigricauda Walshingham, Epinotia exquisitana Christoph and Pseudohedya gradana Christo (all Tortricidae); Cyzenis tetrasetosa sp. nov. ex Lomographa simplicior (Butler), Operophtera brunnea Nakajima and O. relegata Prout (Geometridae); Eulasiona zimini Mesnil ex Archips crataegana (Hübner), Pseudohedya gradana (Christoph), Rhopobota naevana (Hübner) and Rhopobota sp. (all Tortricidae); Panzeria sp. (nr. japonica Shima) ex Erannis golda Djakonov (Geometridae). The genus Eulasiona Townsend is moved to the subfamily Tachininae from its present position in the subfamily Dexiinae, and the female and puparium of Eulasiona zimini Mesnil are described for the first time. The parasitization rates and life habits of these tachinids are briefly discussed.
A Nano-Based P1Cminimum Probe to Monitor Thrombosis in Grafts and Improve Cardiac Function After Coronary Artery Bypass Grafting Surgery
Authors: Wu Qiyong , Tang Xiaoqiang , Shi Haifeng , Zhang Yong , Wang Tao , Yin Ruohan , Pan Changjie
Published: October 2021

Magnetic resonance imaging is widely used to identify and monitor thrombi in grafted vessels following coronary artery bypass grafting surgery (CABG). We produced a biosensor, P1Cm-SPIO-Cy5.5, composed of P1Cm peptide, superparamagnetic iron oxide nanoparticles (SPIO), and polyethylene
glycol (PEG), for use in MRI thrombus imaging. Activated platelets induced by adenosine diphosphate were used for combination tests in vitro. A rat model of common carotid artery thrombosis was used for anti-thrombus experiments in vivo. P1Cm-SPIO-Cy5.5 remains stable in vitro
and has good anticoagulation capacity. It can bind specifically to activated platelets and thrombi. In rats that underwent bypass surgery, P1Cm-SPIO-Cy5.5 could detect and label thrombi over a long period, and prevent thrombosis in grafted vessels. P1Cm-SPIO-Cy5.5 improved cardiac function
in rats following CABG surgery. P1Cm-SPIO-Cy5.5 is a potential sensor for use in MRI for the early diagnosis and prevention of thrombosis after CABG surgery.
Diagnosis of primary ciliary dyskinesia: discrepancy according to different algorithms
Authors: Nussbaumer Mirjam , Kieninger Elisabeth , Tschanz Stefan A. , Savas Sibel T. , Casaulta Carmen , Goutaki Myrofora , Blanchon Sylvain , Jung Andreas , Regamey Nicolas , Kuehni Claudia E. , Latzin Philipp , Müller Loretta
Published: October 2021

Background

Diagnosis of primary ciliary dyskinesia (PCD) is challenging since there is no gold standard test. The European Respiratory (ERS) and American Thoracic (ATS) Societies developed evidence-based diagnostic guidelines with considerable differences.

Objective

We aimed to compare the algorithms published by the ERS and the ATS with each other and with our own PCD-UNIBE algorithm in a clinical setting. Our algorithm is similar to the ERS algorithm with additional immunofluorescence staining. Agreement (Cohen's κ) and concordance between the three algorithms were assessed in patients with suspicion of PCD referred to our diagnostic centre.

Results

In 46 out of 54 patients (85%) the final diagnosis was concordant between all three algorithms (30 PCD negative, 16 PCD positive). In eight patients (15%) PCD diagnosis differed between the algorithms. Five patients (9%) were diagnosed as PCD only by the ATS, one (2%) only by the ERS and PCD-UNIBE, one (2%) only by the ATS and PCD-UNIBE, and one (2%) only by the PCD-UNIBE algorithm. Agreement was substantial between the ERS and the ATS (κ=0.72, 95% CI 0.53–0.92) and the ATS and the PCD-UNIBE (κ=0.73, 95% CI 0.53–0.92) and almost perfect between the ERS and the PCD-UNIBE algorithms (κ=0.92, 95% CI 0.80–1.00).

Conclusion

The different diagnostic algorithms lead to a contradictory diagnosis in a considerable proportion of patients. Thus, an updated, internationally harmonised and standardised PCD diagnostic algorithm is needed to improve diagnostics for these discordant cases.
Complete genomic sequence and phylogenomics analysis of Agrobacterium strain AB2/73: a new Rhizobium species with a unique mega-Ti plasmid
Authors: Hooykaas Marjolein J. G. , Hooykaas Paul J. J.
Published: October 2021

Background

The Agrobacterium strain AB2/73 has a unique host range for the induction of crown gall tumors, and contains an exceptionally large, over 500 kbp mega Ti plasmid. We used whole genome sequencing to fully characterize and comparatively analyze the complex genome of strain AB2/73, including its Ti plasmid and virulence factors.

Results

We obtained a high-quality, full genomic sequence of AB2/73 by a combination of short-read Illumina sequencing and long-read Nanopore sequencing. The AB2/73 genome has a total size of 7,266,754 bp with 59.5% GC for which 7012 genes (6948 protein coding sequences) are predicted. Phylogenetic and comparative genomics analysis revealed that strain AB2/73 does not belong to the genus Agrobacterium, but to a new species in the genus Rhizobium, which is most related to Rhizobium tropici. In addition to the chromosome, the genome consists of 6 plasmids of which the largest two, of more than 1 Mbp, have chromid-like properties. The mega Ti plasmid is 605 kbp in size and contains two, one of which is incomplete, repABC replication units and thus appears to be a cointegrate consisting of about 175 kbp derived from an unknown Ti plasmid linked to 430 kbp from another large plasmid. In pTiAB2/73 we identified a complete set of virulence genes and two T-DNAs. Besides the previously described T-DNA we found a larger, second T-DNA containing a 6b-like onc gene and the acs gene for agrocinopine synthase. Also we identified two clusters of genes responsible for opine catabolism, including an acc-operon for agrocinopine degradation, and genes putatively involved in ridéopine catabolism. The plasmid also harbours tzs, iaaM and iaaH genes for the biosynthesis of the plant growth regulators cytokinin and auxin.

Conclusions

The comparative genomics analysis of the high quality genome of strain AB2/73 provided insight into the unusual phylogeny and genetic composition of the limited host range Agrobacterium strain AB2/73. The description of its unique genomic composition and of all the virulence determinants in pTiAB2/73 will be an invaluable tool for further studies into the special host range properties of this bacterium.
Molecular mechanism of modulating miR482b level in tomato with botrytis cinerea infection
Authors: Wu Fangli , Xu Jinfeng , Gao Tiantian , Huang Diao , Jin Weibo
Published: October 2021

Background

Plant miRNAs are involved in the response to biotic and abiotic stresses by altering their expression levels, and they play an important role in the regulation of plant resistance to stress. However, the molecular mechanism that regulates the expression levels of miRNAs in plants with biotic and abiotic stress still needs to be explored. Previously, we found that the expression of the miR482 family was changed in tomato infected by Botrytis cinerea. In this study, we investigated and uncovered the mechanism underlying the response of miR482 to B. cinerea infection in tomato.

Results

First, RT-qPCR was employed to detect the expression patterns of miR482b in tomato infected by B. cinerea, and results showed that miR482b primary transcripts (pri-miR482b) were up-regulated in B. cinerea-infected leaves, but the mature miR482b was down-regulated. Subsequently, we used rapid amplification cDNA end method to amplify the full-length of pri-miR482b. Result showed that the pri-miR482b had two isoforms, with the longer one (consisting 300 bp) having an extra fragment of 53 bp in the 3’-end compared with the shorter one. In vitro Dicer assay indicated that the longer isoform pri-miR482b-x1 had higher efficiency in the post-transcriptional splicing of miRNA than the shorter isoform pri-miR482b-x2. In addition, the transcription level of mature miR482b was much higher in transgenic Arabidopsis overexpressing pri-miR482b-x1 than that in OE pri-miR482b-x2 Arabidopsis. These results confirmed that this extra 53 bp in pri-miR482b-x1 might play a key role in the miR482b biogenesis of post-transcription processing.

Conclusions

Extra 53 bp in pri-miR482b-x1 enhanced miR482b biogenesis, which elevated the transcription level of miR482b. This study clarified the response of miR482 to B. cinerea infection in tomato, thereby helping us further understand the molecular mechanisms that regulate the expression levels of other miRNAs.
The burden of COVID-19 infection in a rural Tamil Nadu community
Authors: Isaac R. , Paul B. , Finkel M. , Moorthy M. , Venkateswaran S. , Bachmann T. T. , Pinnock H. , Norrie J. , Ramalingam S. , Minz S. , Hansdak S. , Blythe R. , Keller M. , Muliyil J. , Weller D.
Published: October 2021

Background

There have been over 30 million cases of COVID-19 in India and over 430,000 deaths. Transmission rates vary from region to region, and are influenced by many factors including population susceptibility, travel and uptake of preventive measures. To date there have been relatively few studies examining the impact of the pandemic in lower income, rural regions of India. We report on a study examining COVID-19 burden in a rural community in Tamil Nadu.

Methods

The study was undertaken in a population of approximately 130,000 people, served by the Rural Unit of Health and Social Affairs (RUHSA), a community health center of CMC, Vellore. We established and evaluated a COVID-19 PCR-testing programme for symptomatic patients—testing was offered to 350 individuals, and household members of test-positive cases were offered antibody testing. We also undertook two COVID-19 seroprevalence surveys in the same community, amongst 701 randomly-selected individuals.

Results

There were 182 positive tests in the symptomatic population (52.0%). Factors associated with test-positivity were older age, male gender, higher socioeconomic status (SES, as determined by occupation, education and housing), a history of diabetes, contact with a confirmed/suspected case and attending a gathering (such as a religious ceremony, festival or extended family gathering). Amongst test-positive cases, 3 (1.6%) died and 16 (8.8%) suffered a severe illness. Amongst 129 household contacts 40 (31.0%) tested positive. The two seroprevalence surveys showed positivity rates of 2.2% (July/Aug 2020) and 22.0% (Nov 2020). 40 tested positive (31.0%, 95% CI: 23.02 − 38.98). Our estimated infection-to-case ratio was 31.7.

Conclusions

A simple approach using community health workers and a community-based testing clinic can readily identify significant numbers of COVID-19 infections in Indian rural population. There appear, however, to be low rates of death and severe illness, although vulnerable groups may be under-represented in our sample. It’s vital these lower income, rural populations aren’t overlooked in ongoing pandemic monitoring and vaccine roll-out in India.
Clinicopathological characteristics and prognosis of patients with IgA nephropathy and renal vasculitic lesions
Authors: Tang Xueqing , Wen Qiong , Zhou Qian , Chen Wei
Published: October 2021

Background

We studied patients with IgA nephropathy (IgAN) and compared those with and without renal vasculitic lesions (RVLs).

Methods

From January 2006 to December 2011, patients with biopsy-proven primary IgAN at our institution were retrospectively examined and assigned to an RVL group or a no-RVL group. RVLs were defined as thromboses in arteries and/or arterioles, necrosis of capillary loops, crescent formation, and fibrinoid necrosis of small blood vessels. The association of RVLs with clinical outcomes was analyzed using multivariate models. The primary composite endpoint was end-stage renal disease or doubling of serum creatinine.

Results

There were 1570 patients, 50.2% (788) with RVLs and 49.8% (782) without RVLs. The RVL group was younger; had shorter disease course, more severe proteinuria and hematuria, worse renal function; and were prescribed more steroids and/or immunosuppressants. The RVL group had a greater prevalence of global glomerular sclerosis, more crescents, and a higher Oxford classification grade. A total of 501 patients in the RVL group (50.7%) and 487 in the no-RVL group (49.3%) completed follow-up. The RVL group was more likely to reach the composite endpoint after 1, 3, and 5 years (all P < 0.001). Proteinuria, anemia, low eGFR, and global and segmental sclerosis were independent predictors of progression to the composite endpoint in patients with RVLs.

Conclusions

Almost half of our IgAN patients had RVLs, and these patients were younger and had worse renal function, with more severe proteinuria, hematuria, and severe pathologic lesions. IgAN patients with RVLs had worse renal outcomes than those without RVLs.
Differences in the factors associated with tongue pressure between children with class I and Class II malocclusions
Authors: Fujita Yuko , Ohno Yoma , Ohno Keitaro , Takeshima Tomohiro , Maki Kenshi
Published: October 2021

Background

The relationship between tongue pressure and masticatory performance during the mixed dentition period in cases of Class II malocclusion has not been clarified. The aim of this study was to determine differences in tongue pressure-related factors, including maxillofacial morphology and masticatory performance, between Class I and Class II malocclusions during the mixed dentition period.

Methods

A total of 56 children with Class I malocclusion (12 boys, 16 girls) or Class II malocclusion (16 boys, 12 girls) with mixed dentition were included in the present study. Height, body weight, hand grip strength, maximum occlusal force, maximum tongue pressure, masticatory performance, and the number of decayed, missing, and filled teeth were measured in all participants. Their lateral cephalograms were also evaluated. The means of all measurements were compared between Class I and Class II malocclusions. Pearson’s correlation coefficients were used to determine associations between maximum tongue pressure and other variables for each type of malocclusion.

Results

The maximum tongue pressure, hand grip strength, and maximum occlusal force in the Class II malocclusion group were significantly lower than those in the Class I malocclusion group (all, p < 0.05). The maximum tongue pressure was significantly positively correlated with hand grip strength, maximum occlusal force, masticatory performance, and SNB (sella, nasion, B point) angle in the Class I group (all, p < 0.05), and with height, body weight, and labial inclination of the central incisors in the Class II group (all, p < 0.05).

Conclusions

The maxillofacial morphometric factors associated with tongue pressure were clearly different between cases of Class I and Class II malocclusion with mixed dentition. Masticatory performance and tongue pressure were significantly positively correlated in cases of Class I malocclusion, but not in cases of Class II malocclusion.
Process research: compare and contrast the recovery-orientated strengths model of case management and usual community mental health care
Authors: Tse Samson , Ng Catalina S. M. , Yuen Winnie W. Y. , Lo Iris W. K. , Fukui Sadaaki , Goscha Richard J. , Wan Eppie , Wong Stephen , Chan Sau-Kam
Published: October 2021

Background

The strengths model of case management (SMCM), which was developed by Rapp and Goscha through collaborative efforts at the University of Kansas, assists individuals with mental illness in their recovery by mobilizing individual and environmental resources. Increasing evidence has shown that the utilization of the SMCM improves outcomes, including increased employment/educational attainment, reduced hospitalization rates, higher self-efficacy, and hope. However, little is known about the processes through which the SMCM improves outcomes for mental health service users. This study explores the views of case workers and service users on their experience of providing or receiving the SMCM intervention.

Methods

A qualitative design was employed using individual interviews with service users and case workers drawn from two study conditions: the SMCM group and the control group (treatment as usual). For both study conditions, service users were recruited by either centres-in-charge or case workers from integrated community centres for mental wellness (ICCMWs) operated by three non-governmental organizations (NGOs) in different districts of Hong Kong. Through purposeful sampling, 24 service users and 14 case workers from the SMCM and control groups joined the study. We used an inductive approach to analyse the qualitative data.

Results

We identified two overarching themes: service users’ and case workers’ (1) perceptions of the impacts of the interventions (SMCM and control group) and (2) experiences of the interventions, such as features of the interventions and the factors that facilitated the outcomes. The results showed that there were improvements in the functional recovery of the SMCM group in areas such as employment and family relationships, how self-identified goals were achieved, and how service users gained a better understanding of their own strengths and weaknesses. Regarding their experience of the interventions, participants in both the SMCM group and the control group reported that a good relationship between service users and case workers was vital. However, some concerns were raised about the use of SMCM tools, including the strengths assessment and the personal recovery plan (PRP) and the risk of case workers being subjective in the presentation of cases in group supervision sessions.

Conclusion

The results were promising in terms of supporting the use of the SMCM, with some refinements, in mental health services for Chinese clients.

Trial registration

The Australian New Zealand Clinical Trials Registry (ANZCTR), ACTRN12617001435370, registered on 10/10/2017.
Associations of plasma 8-iso-prostaglandin F2αlevels with fasting blood glucose (FBG) and intra-abdominal fat (IAF) area in various Glycometabolism populations
Authors: Ma Ning , Zhang Yujian , Liu Binbin , Jia Xiaojiao , Wang Rui , Lu Qiang
Published: October 2021

Background

This study aimed to investigate the differences in oxidative stress (OS) levels represented by 8-iso-prostaglandin F2α(8-iso-PGF2α) and analyze its correlation with the intra-abdominal fat (IAF) area and the glycolipid index.

Methods

We recruited a total of 160 eligible subjects. According to the blood glucose levels and the T2DM duration, subjects were divided into three groups: Type 2 Diabetes (T2DM) group, Prediabetic group, and Normal glucose-tolerance (NC) group, containing 66, 41, 53 patients, respectively. T2DM groups were additionally divided into a new-onset T2DM group including 29 patients and a non-new-onset T2DM group including 37 patients. General clinical data and biochemical indicators were collected. Intra-abdominal fat (IAF) was measured by MRI. 8-iso-PGF2αwas measured by ELISA.

Results

Compared with the NC group, levels of systolic blood pressure (SBP), waist-to-hip ratio (WHR), FBG, 2 h postprandial glycemia(2hPG), 2 h insulin (2 h INS), IAF area, HOMA-IR, and 8-iso-PGF2αincreased, and high-density lipoprotein cholesterol (HDL-C) decreased in T2DM groups and Prediabetic group (P < 0.05). The 2 h INS level was the highest in the Prediabetic group; 2hPG, and IAF area were the highest in the new-onset T2DM group; WHR, FBG, HOMA-IR and 8-iso-PGF2αwere the highest in the non-new-onset T2DM group. Multiple stepwise regression analysis identified IAF area and FBG as the strongest and independent determinant of 8-iso-PGF2α(P < 0.01).

Conclusions

In various glycometabolism populations, 8-iso-PGF2α is significantly correlated with FBG and IAF, this suggests that high blood glucose and abdominal obesity can increase the damage related to the OS in vivo.
Attributions of survival and methods of coping of long-term ovarian cancer survivors: a qualitative study
Authors: Ketcher Dana , Lutgendorf Susan K. , Leighton Susan , Matzo Marianne , Carter Jeanne , Peddireddy Arjun , Karlan Beth Y. , Tew William P. , Sood Anil K. , Shinn Eileen H.
Published: October 2021

Background

Only 8–23% of advanced epithelial ovarian cancer patients survive for 10 years or longer. Given the need for targeted interventions to improve survival, we interviewed this relatively rare survivor population to gain personalized insights into the reasons for their survival. The aim of this study was to characterize subjective attributions of survival and specific coping mechanisms long-term survivors of ovarian cancer.

Methods

Twenty-two semi-structured, qualitative interviews assessing survival attributions and coping strategies were conducted from April to November 2014. Data were analyzed in a multistep process using ATLAS.ti.8: codes were identified during review of the transcripts and refined with literature review; the frequency of codes and code co-occurrence was calculated, and codes were grouped into themes. Resulting themes were checked by a national leader of an ovarian cancer advocacy organization and compared against available literature.

Results

Thematic analysis found that participants credited their long-term survival to a variety of factors including medical, social, religious/spiritual, and lifestyle/personal characteristics. Some participants rejected these same attributions, concluding that the reason for survival was due to luck or unknowable. Several of Carver et al.’s theoretical dimensions of coping were evident in our sample: planning, positive reinterpretation, social support, religion and acceptance whereas three relatively new strategies were uncovered: conserving emotional energy, value-based activity coping, and self-care.

Conclusions

Long-term survivors’ perspectives were largely consistent with those of newly diagnosed ovarian cancer patients and ovarian cancer survivors of shorter duration. However, the long-term survivors were also willing to reject conventional attributions for survival and recognized the importance of disciplined self-preservational coping strategies.
Alignment in implementation of evidence-based interventions: a scoping review
Authors: Lundmark Robert , Hasson Henna , Richter Anne , Khachatryan Ermine , Åkesson Amanda , Eriksson Leif
Published: October 2021

Background

Alignment (i.e., the process of creating fit between elements of the inner and outer context of an organization or system) in conjunction with implementation of an evidence-based intervention (EBI) has been identified as important for implementation outcomes. However, research evidence has so far not been systematically summarized. The aim of this scoping review is therefore to create an overview of how the concept of alignment has been applied in the EBI implementation literature to provide a starting point for future implementation efforts in health care.

Methods

We searched for peer-reviewed English language articles in four databases (MEDLINE, Cinahl, Embase, and Web of Science) published between 2003 and 2019. Extracted data were analyzed to address the study aims. A qualitative content analysis was carried out for items with more extensive information. The review was reported according to the preferred reporting items for systematic reviews and meta-analyses extension for scoping review (PRISMA-ScR) guidelines.

Results

The database searches yielded 3629 publications, of which 235 were considered potentially relevant based on the predetermined eligibility criteria, and retrieved in full text. In this review, the results of 53 studies are presented. Different definitions and conceptualizations of alignment were found, which in general could be categorized as structural, as well as social, types of alignments. Whereas the majority of studies viewed alignment as important to understand the implementation process, only a few studies actually assessed alignment. Outcomes of alignment were focused on either EBI implementation, EBI sustainment, or healthcare procedures. Different actors were identified as important for creating alignment and five overall strategies were found for achieving alignment.

Conclusions

Although investigating alignment has not been the primary focus of studies focusing on EBI implementation, it has still been identified as an important factor for the implementation success. Based on the findings from this review, future research should incorporate alignment and put a stronger emphasize on testing the effectiveness of alignment related to implementation outcomes.
Effects of family-based dignity intervention and expressive writing on anticipatory grief of family caregivers of patients with cancer: a study protocol for a four-arm randomized controlled trial and a qualitative process evaluation
Authors: Seyedfatemi Naima , Ghezeljeh Tahereh Najafi , Bolhari Jafar , Rezaei Masoud
Published: October 2021

Background

Family caregivers of dying cancer patients are affected by grief experiences and bereavement complications. Several approaches such as psycho-emotional care and an increase in spirituality have been suggested to diminish these complications. However, the knowledge about the effects of family-based dignity intervention and expressive writing on anticipatory grief in family caregivers of dying cancer patients is limited. This is a study protocol describing a hospital-based mixed-methods study on the effects of family-based dignity intervention and expressive writing on anticipatory grief in family caregivers of dying cancer patients.

Methods

This mixed-methods study will be done in an embedded explanatory design with two quantitative and qualitative phases. In the first phase (quantitative), a randomized clinical trial will be done, in which 200 family caregivers of dying cancer patients will be randomly assigned to one of the four groups: family-based single dignity intervention (group 1), expressive writing intervention (group 2), combined family-based single dignity intervention and expressive writing (group 3), and control (group 4). At baseline, 1 week and 2 weeks after the interventions, anticipatory grief will be assessed by a 13-item anticipatory grief scale. After the quantitative phase, the qualitative phase will be conducted through the conventional content analysis approach of Granheim and Lundman, in which an individual semi-structured interview will be taken from participants in the first phase to collect data on their experiences on interventions. Finally, data from the quantitative and qualitative phases will be analyzed and discussed.

Discussion

Family caregivers of dying cancer patients usually experience depression, anxiety, and psychological distress due to isolation and inadequate social support. Psychological interventions such as dignity and expressive writing interventions may help caregivers to obtain a better understanding of themselves and to increase their abilities to cope with caregiving difficulties. Therefore, there is a need for a comprehensive study confirming the effects of mentioned interventions on family caregivers of dying cancer patients.

Trial registration

Iranian Registry of Clinical Trials (www.irct.ir) identifier: IRCT20210111050010N1. Date of trial registration: Feb 6, 2021. This is the first version of this protocol.
Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector
Authors: de Paula Adriano Rodrigues , Silva Leila Eid Imad , Ribeiro Anderson , da Silva Gerson Adriano , Silva Carlos Peres , Butt Tariq M. , Samuels Richard Ian
Published: October 2021

Background

The use of entomopathogenic fungi (EPF) for the control of adult mosquitoes is a promising alternative to synthetic insecticides. Previous studies have only evaluated conidiospores against adult mosquitoes. However, blastospores, which are highly virulent against mosquito larvae and pupae, could also be effective against adults.

Methods

Metarhizium anisopliae (ESALQ 818 and LEF 2000) blastospores and conidia were first tested against adult Aedes aegypti by spraying insects with spore suspensions. Blastospores were then tested using an indirect contact bioassay, exposing mosquitoes to fungus-impregnated cloths. Virulence when using blastospores suspended in 20% sunflower oil was also investigated.

Results

Female mosquitoes sprayed with blastospores or conidia at a concentration of 108 propagules ml−1 were highly susceptible to both types of spores, resulting in 100% mortality within 7 days. However, significant differences in virulence of the isolates and propagules became apparent at 107 spores ml−1, with ESALQ 818 blastospores being more virulent than LEF 2000 blastospores. ESALQ 818 blastospores were highly virulent when mosquitoes were exposed to black cotton cloths impregnated with blastospores shortly after preparing the suspensions, but virulence declined rapidly 12 h post-application. The addition of vegetable oil to blastospores helped maintain virulence for up to 48 h.

Conclusion

The results showed that blastospores were more virulent to adult female Ae. aegypti than conidia when sprayed onto the insects or applied to black cloths. Vegetable oil helped maintain blastospore virulence. The results show that blastospores have potential for use in integrated vector management, although new formulations and drying techniques need to be investigated.

Graphical abstract
Insights from Leishmania (Viannia) guyanensis in vitro behavior and intercellular communication
Authors: Pereira Luiza O. R. , Sousa Cíntia S. , Ramos Hellen C. P. , Torres-Santos Eduardo C. , Pinheiro Liliane S. , Alves Marcelo R. , Cuervo Patricia , Romero Gustavo A. Sierra , Boité Mariana C. , Porrozzi Renato , Cupolillo Elisa
Published: October 2021

Background

Pentavalent antimonial-based chemotherapy is the first-line approach for leishmaniasis treatment and disease control. Nevertheless antimony-resistant parasites have been reported in some endemic regions. Treatment refractoriness is complex and is associated with patient- and parasite-related variables. Although amastigotes are the parasite stage in the vertebrate host and, thus, exposed to the drug, the stress caused by trivalent antimony in promastigotes has been shown to promote significant modification in expression of several genes involved in various biological processes, which will ultimately affect parasite behavior. Leishmania (Viannia) guyanensis is one of the main etiological agents in the Amazon Basin region, with a high relapse rate (approximately 25%).

Methods

Herein, we conducted several in vitro analyses with L. (V.) guyanensis strains derived from cured and refractory patients after treatment with standardized antimonial therapeutic schemes, in addition to a drug-resistant in vitro-selected strain. Drug sensitivity assessed through Sb(III) half-maximal inhibitory concentration (IC50) assays, growth patterns (with and without drug pressure) and metacyclic-like percentages were determined for all strains and compared to treatment outcomes. Finally, co-cultivation without intercellular contact was followed by parasitic density and Sb(III) IC50 measurements.

Results

Poor treatment response was correlated with increased Sb(III) IC50 values. The decrease in drug sensitivity was associated with a reduced cell replication rate, increased in vitro growth ability, and higher metacyclic-like proportion. Additionally, in vitro co-cultivation assays demonstrated that intercellular communication enabled lower drug sensitivity and enhanced in vitro growth ability, regardless of direct cell contact.

Conclusions

Data concerning drug sensitivity in the Viannia subgenus are emerging, and L. (V.) guyanensis plays a pivotal epidemiological role in Latin America. Therefore, investigating the parasitic features potentially related to relapses is urgent. Altogether, the data presented here indicate that all tested strains of L. (V.) guyanensis displayed an association between treatment outcome and in vitro parameters, especially the drug sensitivity. Remarkably, sharing enhanced growth ability and decreased drug sensitivity, without intercellular communication, were demonstrated.

Graphical Abstract
Designing a sustainable biofuel supply chain by considering carbon policies: a case study in Iran
Authors: Zarrinpoor Naeme , Khani Aida
Published: October 2021

Background

Carbon emissions and global warming have increased as a result of population growth and greater usage of fossil fuels. Finding a long-term replacement for fossil fuels, such as biofuels, has become a major problem for energy supply management in recent years. Sustainability must be addressed as a key problem in building biofuel supply chains (BSCs), given the pressing need for societies to limit environmental consequences and promote social responsibility of company activities. Various modeling frameworks have been established so far to design a BSC. At the same time, no research exists that examines both the sustainable development paradigm and the influence of various carbon regulatory policies on the strategic and operational decisions made by BSCs.

Methods

This study develops a multi-objective, multi-period, multi-echelon BSC from switch grass regarding the economic, environmental and social aspects of sustainability. Four carbon policies are taken into account when assessing the environmental aspect: the carbon cap, the carbon tax, the carbon trade, and the carbon offset. To solve the multi-objective model, the fuzzy interactive programming method is used, and the fuzzy best–worst method is used to weight the social objective components.

Results

An actual case study in Iran is studied to demonstrate the model’s applicability. Under various carbon policies, different network configurations are obtained based on the location of switch grass resources and installed facilities. Biofuel production and transportation activities account for approximately 28% and 51% of total carbon emissions, respectively, according to numerical results. Furthermore, these activities account for roughly 62% of overall expenses. In the suggested case study, implementing the carbon trade policy reduces carbon emissions by more than 30% while increasing total profit by about 27%. In comparison to other policies, the carbon trade policy has a substantial impact on enhancing social considerations. Overall, the carbon trade policy can greatly improve the economic and environmental components of sustainability without significantly decreasing in the social sustainability.

Conclusions

The proposed model can assist policymakers and governments in simultaneously optimizing BSC profitability, carbon emission reduction, and social concern.
Long-term outcome of postpartum psychosis: a prospective clinical cohort study in 106 women
Authors: Rommel Anna-Sophie , Molenaar Nina Maren , Gilden Janneke , Kushner Steven A. , Westerbeek Nicola J. , Kamperman Astrid M. , Bergink Veerle
Published: October 2021

Objective

We aimed to investigate the outcome of postpartum psychosis over a four-year follow-up, and to identify potential clinical markers of mood/psychotic episodes outside of the postpartum period.

Methods

One hundred and six women with a diagnosis of first-onset mania or psychosis during the postpartum period were included in this prospective longitudinal study. Women were categorized into either (1) recurrence of non-postpartum mood/psychotic episodes or (2) mania/psychosis limited to the postpartum period. We summarize the longitudinal course of the illness per group. We used a logistic regression model to identify clinical predictors of recurrence of mood/psychotic episodes outside of the postpartum period.

Results

Over two thirds of the women included in this study did not have major psychiatric episodes outside of the postpartum period during follow-up. The overall recurrence rate of mood/psychotic episodes outside the postpartum period was ~ 32%. Of these women, most transitioned to a bipolar disorder diagnosis. None of the women fulfilled diagnostic criteria for schizophrenia or schizophreniform disorder. No clinical markers significantly predicted recurrence outside of the postpartum period.

Conclusions

For the majority of women with first-onset postpartum psychosis, the risk of illness was limited to the period after childbirth. For the remaining women, postpartum psychosis was part of a mood/psychotic disorder with severe non-postpartum recurrence, mainly in the bipolar spectrum. No clinical predictors for risk of severe episodes outside the postpartum period emerged. Our findings add to previous evidence suggesting a fundamental link between postpartum psychosis and bipolar disorder, which may represent two distinct diagnoses within the same spectrum.