bluuebunny/crossref_metadata_2025 huggingface dataset:
Ruegeria pomeroyi DSS-3 gene annotations - April 2020 v1
Authors: Smith Christa , Ann Moran Mary
Published: April 2020

Ruegeria pomeroyi DSS-3 is an alphaproteobacterium isolated in 1998 from coastal Georgia, USA, seawater by enriching for growth on the organic sulfur compound dimethylsulfoniopropionate (DMSP) (González and Moran 1997, Buchan et al. 2001). R. pomeroyi is a member of the Roseobacter group, which consists of marine bacteria within the family Rhodobacteraceae that are important members of bacterioplankton communities in coastal and open oceans. R. pomeroyi grows quickly in the laboratory and has a genetic system. Since its genome sequence became available in 2004 (Moran et al., 2004), R. pomeroyi has been serving as a model organism for studies of the biogeochemical, ecological, and physiological strategies of heterotrophic marine bacteria. This document catalogs the current manually curated gene annotations of the Ruegeria pomeroyi DSS-3 genome, updated as of April, 2020.
TissueCyte Specimen Embedding Acrylamide Coembedding v3
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol describes the preparation of a fixed mouse brain specimen bonded to an agarose block for blockface imaging and sectioning using the TissueCyte 1000 system. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
RNA extraction and precipitation for 47 mm PES and polycarbonate filters v2
Authors: Ferrer-Gonzalez Frank , Smith Christa
Published: April 2020

This protocol is a modified version of Bryn Durham et al., 2015 for extracting RNA from filters. This protocol has been tested with 0.22 μm pore size Durapore filters and 2.0 μm polycarbonate filters. Custom synthesized RNA transcript standards are added at the time of extraction and are recovered post-sequencing for quantitative metatranscriptome analysis (Satinsky et al., 2013). To prevent RNA degradation, work in a clean environment with asceptic practices and avoid possible introduction of RNases to samples and reagents.
FACS Single Cell Sorting v3
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol provides step by step instructions for performing single-cell sorting by using BD FACSAria II machine or BD FACSAria Fusion machine and BD FACSDiva software. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Patch-Seq Internal Solution with Biocytin v3
Authors: Institute for Brain Science Allen
Published: April 2020

This SOP provides instruction to prepare Internal Solution which is used for patch clamp electrophysiology, modified for cleanliness and mRNA capture and sustainability. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
SARS-CoV-2 Enrichment Sequencing by Spiked Primer MSSPE method v4
Authors: E. Manning Jessica , Bohl Jennifer , Lay Sreyngim , Chea Sophana , Ahyong Vida , Karlsson Erik
Published: April 2020

This protocol was used to enrich for SARS-CoV2 sequencing reads from a confirmed COVID-19 swab sample and attain the full genome using an iSeq100. By using a spiked primer approach with 73 primers spanning the entire SARS-CoV2 genome, we were able to get an average of 15x genome coverage on an iSeq100 with 1.8 million paired end-reads. Here we overview all the steps, from sample extraction, library preparation with a spiked primer enrichment step, and sequencing on an iSeq100. The collaborative effort involved the Manning Lab, NIAID in Phnom Penh, Cambodia, Institut Pasteur Cambodge, Cambodia Ministry of Health, the Chan Zuckerberg Biohub, and the Chan Zuckerberg Initiative. This approach was developed based on the work from Deng et al, Nature Microbiology, January 13, 2020. https://www.nature.com/articles/s41564-019-0637-9
Brain Dissection of Post-natal Mice v3
Authors: Institute for Brain Science Allen
Published: April 2020

This protocol describes the procedure for removal and embedding of the post-natal mouse brain. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
The benefits of early surgical treatment of deep cervical burns in children
Authors: Gnipov Pavel A. , Baindurashvili Alexey G. , Brazol Marina A. , Mitrofanova Ekaterina V. , Melnikov Maxim R. , Mashevskiy Gleb A.
Published: April 2020

Background. The frequency of deep cervical burns in children is four times higher than that of deep face burns. Currently, there is no consensus on the methods for surgical treatment of deep burns in cervical areas; meshed skin autografts continue to be used.
Aim. To evaluate the benefits of early surgical treatment of deep сervical burns in children between the third and fifth days from the moment of injury.
Materials and methods. Case-control study. Surgical treatment was performed in 81 children with deep cervical burns. The main group with early surgical treatment included 46 children and underwent surgical treatment at 3.37 0.14 days from the moment of injury; the control group received autograft during stage treatment for 35 children at 27.17 0.18 days. The treatment results were evaluated by the following indicators: the number of dressing changes, the period of skin restoration, and the area of graft success. In the long term, functional and cosmetic treatment results were evaluated.
Results. In the study and control groups, 7.93 0.45 and 18.75 0.61 dressings were required to complete the treatment, respectively (p 0.001). The skin restoration periods were 16.54 0.68 and 36.94 0.89 days, respectively (p 0.001). The graft success areas were 99.50% 0.13% in the main group and 93.91% 2.68% in the control (p 0.001). During the staged surgical treatment, one patient showed a loss of 90% of the graft, which required regrafting. Other complications in the treatment process have not been noted. When assessing long-term cosmetic results using the Vancouver Scar Scale, the average score was 4.0 0.26 points in the main group and 7 0.28 points in the control (p 0.001). The presence of post-burn cicatricial contracture in the main group was noted in 12 (26%) people and the absence in 34 (74%) children. In the control group, 20 (57%) patients required surgical removal of post-burn deformity, and 15 (43%) children did not need further surgical interventions.
Conclusions. Early surgical treatment of deep cervical burns in children on the third and fifth days from injury allows not only to accelerate the process of restoration of the skin but also to directly affect the cosmetic and functional results in a better way.
Effect of brachial plexus block on the incidence of arterial hypotension and bradycardia events during shoulder arthroscopy in adolescents
Authors: Trukhin Konstantin S. , Zabolotskii Dmitrii V. , Koriachkin Viktor A. , Kuleshov Oleg V. , Zakharov Kirill I. , Сherednichenko Andrei A. , Kulikov Aleksei Yu.
Published: April 2020

Background. The role and significance of the technical aspects of interscalene brachial plexus block in the occurrence of sudden arterial hypotension and bradycardia events during shoulder arthroscopy in a semi-sitting position are ambiguous.
Aim. The study aimed to assess the effect of interscalene brachial plexus block on the incidence of hypotension-bradycardia events during shoulder arthroscopic surgery in adolescents in a semi-sitting position.
Materials and methods. This retrospective analysis of anesthesia protocols included 288 patients who underwent arthroscopic shoulder surgery in a semi-sitting position under the interscalene brachial plexus block. Regional blockades were performed with neurostimulation in Group 1 (n = 23), neurostimulation and ultrasound navigation without repositioning the needle in Group 2 (n = 70), and neurostimulation and ultrasound navigation with multiple precision repositioning the needle in Group 3 (n = 195).
Results. Hypotension-bradycardia events were detected in 26 patients out of 288 (9%). There was a statistically significant difference in the frequency of hypotension-bradycardia in all groups: 10 (43.48%) in Group 1, 15 (21.43%) in Group 2, and 1 (0.51%) in Group 3 (p = 0.000). A direct correlation between hypotension-bradycardia episodes and local anesthetic volume (r = 0.405; p 0.05), and Horners syndrome (r = 0.684, p 0.05) was found.
Conclusions. Interscalene brachial plexus block with a target delivery of low volume of local anesthetic and dual navigation reduces the risk of hypotension-bradycardia. Horners syndrome can be considered an early predictor of hypotension-bradycardia events.
Extension of the Basin Rayleigh-Wave Amplification Theory to Include Basin-Edge Effects
Authors: Brissaud Quentin , Bowden Daniel C. , Tsai Victor C.
Published: April 2020

The presence of sediments near the Earth’s surface can significantly amplify the strength of shaking during earthquakes. Such basin or site amplification effects have been well documented in numerous regions, yet the complex and often situational dependence of competing reasons for this amplification makes it hard to quantify in a general sense or to determine the most significant contributions. Simple 1D seismic profiles can be used to estimate the amplitude differences between a basin site and a hard-rock reference site, but this ignores any reflections or conversions at the basin edge or a resonance effect depending on the basin’s geometry. In this article, we explore an analytic model based on coupling coefficients for surface Rayleigh waves to account for the lateral discontinuities at a basin’s edge (Datta 2018). We use this simple tool to explore the relationship between the basin’s Rayleigh-wave amplification spectrum and various parameters such as basin depth, edge slope angle, and impedance contrast. The step-by-step construction of the model allows us to quantify the contributions from various wave propagation effects with the goal of identifying situations under which various basin-edge effects must be considered in addition to purely 1D estimates. For the most velocity contrasts (less than a factor of 5), the error made by the 1D theory in predicting maximum Rayleigh-wave basin amplification is under 35% for both the horizontal and the vertical components. For simple basins, the vertical amplification dominates at larger high frequencies and the horizontal at lower frequencies. Finally, we demonstrate from comparisons with spectral-element wavefield simulations that realistic velocity structures can be reduced to a simpler “box” shape for the semi-analytic formulation used here with reasonable results. For the purposes of estimating site-amplification or microzonation, an improved model that accounts for basin-edge effects can be implemented without high-computational cost.
Role of peritoneal drainage in moribund patients of perforation peritonitis
Authors: Jaiswal Sakshi , Sharma Subhash Chandra
Published: April 2020

Background: Objective of the present study was to evaluate applicability of primary peritoneal drainage under local anesthesia in moribund patients as pre-laparotomy support when laparotomy under general anesthesia could not be done. Also to assess outcome in terms of survival, patients needing definitive surgery and complications associated with the procedure on immediate and follow up basis.Methods: We conducted this study in Teerthanker Mahaveer Medical College, Moradabad, UP, India from October 18 to October 2019. 71 patients were admitted as cases of perforation peritonitis in moribund condition, demographic data of all patients was noted, peritoneal drainage under local anesthesia in flanks was done and variable amount of fluid was drained in different patients, simple parameters were taken in to consideration in pre and post drainage phase. We also noted the complications after the definitive surgery.Results: Out of 71 patients, admitted in late and in very critical state, 61 patients improved after drainage and resuscitative procedures, although in ASA grade 3 and 4 they underwent surgical treatment and we were able to save their lives. 13 patients expired after definitive surgery inspite of best efforts.Conclusions: Peritoneal drainage under local anesthesia in late reporting and critical patients, not only improves general condition but makes patients better to undergo further surgery, and can prove to be life saver. It being a simple procedure can easily be done at even PHC level, before patient is referred or shifted to higher centre for further management.
RE-IMAGINING CHILD AND YOUTH CARE PRACTICE WITH AFRICAN CANADIAN YOUTH
Authors: Daniel Beverly-Jean , Jean-Pierre Johanne
Published: April 2020

This article is based on a plenary held during the Child & Youth Care in Action VI Conference: Moving Through Trails and Trials Toward Community Wellness, held in Victoria, British Columbia in April 2019. It explores how we can re-imagine child and youth care practice with African Canadian youth. This emerging paradigm aligns with child and youth care politicized praxis as well as trauma-informed and strengths-based approaches in the field’s literature. We highlight the importance of mobilizing critical and transformative theoretical frameworks along with an Africentric worldview to substantially support youth of African descent with a strengths-based approach. Moreover, the protective role of Black-affirming spaces is developed and articulated.
Cinética de secagem e sua influência nas dimensões de sementes de abóbora sem casca
Authors: Vieira Danise Medeiros , Barros Sâmela Leal , Silva Vírgínia Mirtes de Alcântara , Santos Newton Carlos , Nascimento Amanda Priscila Silva , Melo Mylena Olga Pessoa
Published: April 2020

A secagem aplicada as sementes de abóbora, proporciona o aumento da vida de prateleira do produto e consequentemente facilita a sua comercialização. Objetivou-se através do presente estudo realizar a cinética de secagem das sementes de abóbora sem cascas em diferentes temperaturas do ar de secagem, ajustando os dados experimentais obtidos à modelos matemáticos empíricos, avaliando a influência da secagem em suas dimensões. A cinética de secagem das sementes de abóbora sem casca foi realizada em temperaturas do ar de secagem (50, 70 e 90 °C), ajustando os dados à modelos matemáticos empíricos de Handerson e Pabis, Lewis e Page. O tamanho das sementes foi analisado quanto ao comprimento, largura, espessura com auxílio de paquímetro digital. Com relação aos modelos matemáticos aplicados, o modelo de Page foi considerado como sendo mais eficiente na descrição do processo de secagem de sementes de abóbora sem casca. Constatou-se que as dimensões das sementes foram diretamente influenciadas pelo aumento da temperatura de secagem. Portanto, o aumento da temperatura do ar de secagem aplicado proporciona maior remoção de umidade do produto e os dados de suas dimensões podem ser usados para dimensionar o tamanho de peneiras e equipamentos de pós-colheita.
Real Scientific Index (RSI): A Totally New Approach in Quantifying the Real Academic Value of an Author in the Contemporary Literature
Authors: Nashibi Masoud , Nashibi Amin , Javadi Seyed Amir , Hussain Khan Zahid
Published: April 2020

This article is an Editorial and does not include an Abstract.
Comparison of Labetalol and Nitroglycerine on Intraoperative Bleeding in Patients Who Underwent Dacryocystorhinostomy
Authors: Sanatkar Mehdi , Ebrahim Soltani Alireza , Takzare Alireza
Published: April 2020

The bleeding during dacryocystorhinostomy (DCR) surgery is one of the main complications that led to the surgeon’s dissatisfaction and increased the duration of the operation. The current study aimed at comparing the effects of labetalol and nitroglycerine (TNG) on blood loss and the surgeon’s satisfaction during DCR. The current prospective and randomized study enrolled 60 patients candidate for DCR under local anesthesia and sedation and divided into two groups. When the surgeon dissatisfied with bleeding during the operation, patients in the labetalol group received labetalol infusion at a rate of 0.5-2 mg/kg and compared with the subjects in the TNG group that received TNG infusion at a rate of 0.1 µg/kg/min. Additionally, the surgical condition was assessed by the surgeon using the average category scale (ACS) and surgeon’s satisfaction by a scoring system. The average bleeding in the labetalol group was 140.5±24.5 ml versus 170.4±24.6 ml in the TNG group, respectively (P=0.001). The average category scale for the labetalol group was better than the TNG group during all the time of operation. The surgeon satisfaction score during the operation was more in the labetalol group (3.4 in the labetalol group versus 2.8 in the TNG group, respectively). Labetalol was better than TNG for controlling bleeding during DCR procedure because of decreasing surgical blood loss and optimum operative condition.
Lower Circulating Levels of Soluble TNF-Like Weak Inducer of Apoptosis and Chromium in Treated Brucella Infection
Authors: Mohsenpour Azari Mohammad , Golestani Eimani Bahram , Entezarmahdi Rasoul
Published: April 2020

Brucellosis is a common infectious disease around the globe. The aim of the present investigation was to evaluate the effect of brucellosis on the serum levels of chromium (Cr), lead (Pb), soluble TNF–like weak inducer of apoptosis (sTWEAK) as well as high-sensitivity C-reactive protein (hs-CRP) and homocysteine in patients suffering from brucellosis. The present case-control study was executed on 40 treated brucellosis patients (case) and 40 healthy individuals (control). Blood samples were obtained from all the participants for the measurement of the desired indices. The serum levels of chromium in the case group (0.109±0.025 μg/L) was significantly lower compared with that in the control group (0.121±0.027 μg/L, P=0.047). The levels of lead in serum were almost unchanged between the two groups (12.58±1.94 vs. 12.28±2.42 μg/dL, P=0.533). The sTWEAK levels were significantly lower in the case group as compared to the control subjects (235.20±48.45 pg/mL vs. 262.00±67.25 pg/mL, P=0.044). The levels of hs-CRP were significantly higher in the case group (2.23±0.34 mg/L) than that in the control group (2.05±0.32 mg/L, P=0.016); similarly, homocysteine levels were higher in the serum of patients in case group as compared to the control ones (16.18±4.47 µmol/L vs. 14.36±3.06 µmol/L, P=0.038). The current investigation underlined that brucellosis causes alterations in serum chromium and sTWEAK levels, the markers that are considered as the predictors of cardiovascular disease.
Association Between Forced Expiratory Volume in one Second and Glycated Hemoglobin Values in Patients With Chronic Obstructive Pulmonary Disease
Authors: Kheirandish Masoumeh , Shahbazi Roghayeh , Azimzadeh Aida , Mahmoodi Masoumeh , Dehestani Vahid
Published: April 2020

Chronic Obstructive Pulmonary Disease (COPD) is one of the leading causes of mortality worldwide. Evidence shows that COPD increases the risk of type 2 diabetes possibly due to insulin resistance induced by inflammatory cytokines. The aim of the current study was to evaluate the association between forced expiratory volume in one second (FEV1) and plasma glycated hemoglobin (HbA1c) level in patients with COPD. In this study, 50 non-hospitalized patients with COPD were studied. For all patients, a spirometry test was performed and FEV1 was determined. The quality of spirometry was assessed based on Guidelines from the American Thoracic Society/European Respiratory Society Task Force and the severity of COPD was determined based on GOLD criteria. HbA1c was measured by commercial kits. Anthropometric indices were measured and a questionnaire was applied to collect general characteristics of patients. The mean age of patients was 60.18±7.63 years. Seventy-eight percent and 22% of patients were male and female, respectively. Twenty-seven subjects were current smokers and 23 subjects were non-smokers. A significant inverses association was found between HbA1c level and FEV1% in (r= -0.722, P<0.001). There was a statistically significant correlation between weight and HbA1c level (r=0.349, P<0.05), and BMI and HbA1c (r=0.242, P<0.05). We could not find any significant correlation between age and smoking and the level of HbA1c and FEV1 (P>0.05). Our study showed there is a negative correlation between the level of HgA1c and respiratory function in COPD patients
Non-Hodgkin's Primary Lymphoma in the Base of Tongue: A Case Report
Authors: Dehghan Parvaneh , Kakhki Samaneh , Eshaghzadeh Maliheh , Khezri Mohammad Amin
Published: April 2020

Although the most common site in Non-Hodgkin's Lymphoma (NHL) is the Waldeyer’s ring, the involvement of the tongue is extremely rare. The present study revealed a 70-year-old female with primary non‑Hodgkin’s lymphoma (NHL) of the base of the tongue with the chief complaint of sore throat and dysphasia as a rare case report. The patient was expired 3 months after diagnosis because of the advanced stage of the disease and poor physical statue. The diagnosis was confirmed as primary NHL diffuse large B cell type by histopathological and immunohistochemistry results
The Effect of 8 weeks Aerobic Training and Glycogon Consumption on Serum Apelin and Insulin Resistance in Women with Type 2 Diabetes
Authors: Ramezani Mahboobeh , Taghian Farzaneh
Published: April 2020

Objective: Apelin is a novel adipokine that plays an important role in regulating energy homeostasis and improving insulin resistance. The purpose of the present study was to compare the effect of eight weeks aerobic interval training and glycogon consumption on serum apelin levels and insulin resistance in women with type 2 diabetes. Materials and Methods: This is a quasi-experimental study. Forty diabetic women with mean age (48.08± 6.14) weight (72.61± 11.51) kg, height (156.33± 6.36) cm, body mass index (BMI) (29.73 ± 4.62) kg / m 2 were selected. Samples were randomly divided into four groups: aerobic interval training (n= 10), glycogon consumption (n= 10), combination (n= 10) and control (n= 10). To measure low density lipoprotein (LDL), high density lipoprotein (HDL), triglyceride (TG), fasting blood glucose, cholesterol, insulin, apelin and insulin resistance were measured. Then the aerobic and combination group participated in an aerobic interval training program (8 weeks, 3 sessions per week, 1 hour session, 40-60% of maximal beat intensity). Glycogel 3 capsules of 145 mg daily were used. After eight weeks, all variables were measured in four groups. T-test and analysis of variance were used. Results: The findings of this study showed that the level of apelin decreased significantly in all three groups (P-value=0.018). Changes in glucose (P-value=0.16) and insulin (P-value=0.31) in groups were not significant .Insulin resistance was significantly decreased in the combination and glycogel groups (P-value=0.03). Conclusion: Aerobic interval exercises and glycogon consumption can be used to reduce some of the risk factors for diabetes in people with diabetes.
Effect of 6 Weeks of High Intensity Interval Training with Nano curcumin Supplement on Antioxidant Defense and Lipid Peroxidation in Overweight Girls- Clinical Trial
Authors: Fakhri Somaye , Shakeryan Saeed , Alizadeh Aliakbar , Shahryari Ali
Published: April 2020

Objective: Thepurpose of this study was to investigate the effect of 6 weeks of HIIT training combining nano-curcumin supplement on antioxidant defense and lipid degradation in overweight girls. Materials and Methods: The research method was semiexperimental study. Accordingly, 48 overweight girl students were randomly divided into four groups: training (n=12), training-supplement (n=12), supplement (n=12) and control group (n=12). Supplement groups consumed 80 mg nano-curcumin capsule daily. Training groups performed an exercise protocol of HIIT training with maximum heart rate for 6 weeks (three sessions per week). The control group did not have any regular exercise. Blood samples were obtained before and after training period for antioxidant indicators and lipid degradation measurement. T-test and one-way analysis of variance were used for the evaluation of within-group and between-group differences, respectively. Results: A significant increase was observed in serum levels of Malondialdehyde ( P -value= 0.004) in the training group after 6 weeks. Also, there was a significant increase in serum Glutathione ( P -value= 0.001), Superoxide dismutase ( P -value= 0.006) and Catalase indexes ( P value= 0.01) in the supplement group. Moreover, a significant increase in catalase ( P -value= 0.001), glutathione ( P -value= 0.006), superoxide dismutase ( P -value= 0.015) and glutathione peroxidase indexes ( P value= 0.05) and a significant decrease in malondialdehyde ( P -value= 0.009) were observed in the training supplement group. Conclusion: A positive antioxidant effect was seen, so taking curcumin supplement along with exercises may have beneficial effects on reinforcement the antioxidant system and prevention of lipid peroxidation in overweight individuals.
The Effect of Endurance Training Intensity on the Expression of Perlipin a Protein of Subcutaneous Adipose Tissue and Pancreatic B cells Function in Diabetic Rats
Authors: Ghaedi Hadi
Published: April 2020

Objective: The aim of this study was to compare the three endurance training intensities on the expression of Perlipin A protein in subcutaneous adipose tissue and pancreatic B-cells function in male diabetic rats. Materials and Methods: In this study, 40 healthy male wistar rats were divided into five groups, including diabetic with low intensity endurance training, diabetic with moderate intensity endurance training, diabetic with high intensity endurance training, diabetic control and healthy control. After diabetic induction with streptozotocin, endurance training was performed with low intensity, moderate and severe for eight weeks, three sessions per week. The relative expression of Perilipin A was measured by western blot technique. Results: The results indicated a significant effect of endurance training with three intensities on serum levels of insulin and glucose and pancreatic B-cells function ( P -value: 0.001). Also the results showed that there was no significant difference between Perlipin A expression in healthy and diabetic control groups with endurance training groups (with low, moderate and high intensity) ( P -value: 0.07). Conclusion: However, Moderate and high intensity endurance training compared to low-intensity training can compensate for the loss caused by diabetes in the expression of the Perlipin A protein but the difference was not significant. It seems that more intensity endurance training lead to more increase in Perlipin A expression in diabetic rats.
The Effects of Continuous and High Intensity Interval Trainings on Plasma Betatrophin Level in Diabetic Rats Treated with Metformin
Authors: Mahmoudi Yadollah , Gholami Mandana , Nikbakht Hojatolah , Ebrahim Khosrow , Bakhtiyari Salar
Published: April 2020

Objective: The study aimed to determine the effect of eight weeks high intensity interval (HIIT) and sub-maximal continuous trainings on plasma betatrophin level in diabetic rats treated with metformin. Materials and Methods: In this experimental study, 42 diabeticwistar rats were divided into six groups (n=7): diabetic control (C), diabetic control + metformin consumption(C+M), diabetic HIIT, diabetic HIIT + metformin (HIIT+M), diabetic sub-maximal continuous training (SMCT), and diabetic sub-maximal continuous training + metformin (SMCT+M). Metformin was given 150 mg/kg/day by gavage every day, 48 hours after the end of the last training session, the rats were sacrificed. Then blood glucose and glycated hemoglobin (HbA1c) levels were measured. One-way ANOVA test was used for statistical analysis of data. Results: The level of plasma betatrophin was significantly different in the HIIT ( P -value= 0.01) and C+M ( P -value= 0.001) groups compared to C group. Blood glucose was significantly decreased in all training groups with or without betatrophin compared with the diabetic control group ( P -value= 0.001). However, there were no significant changes between glucose levels in HIIT, HIIT+M, SMCT, and SMCT+M groups but SMCT showed most reduction in blood glucose. Conclusion: Treatment with metformin did not change blood glucose but two types of exercise training with high and moderate intensity reduced blood glucose thus exercise can be a good alternative modality rather than taking medicine.
The Effect of 12 Weeks Resistance Training on FOXO1 Expression in Hepatocytes, Glucose and Insulin in Diabetic Rats- A Brief-Report
Authors: Yarmohammadi Masoomeh , Behboudi Laleh , Eizadi Mojtaba
Published: April 2020

Objective: In diabetic patients, hyperglycemia is associated with impaired FOXO signaling pathways in liver cells. This study aimed to determine the effect of resistance training on FOXO1 expression in liver hepatocytes and fasting glucose levels in type 2 diabetic rats. Materials and Methods: In this experimental study, type 2 diabetes induced by intraperitoneal injection of nicotinamide-STZ in 16 male wistar rats (220±20 g) and were randomly divided into exercise (n=8) and control (n=8) groups. Exercise group were participated in resistance training program (12 weeks, 5 days/weekly). Fasting glucose and insulin as well as FOXO1 expressioninliver hepatocytes were measured lasted exercise session of 2 groups and compared by independent T-test. Results: Compared to control group, resistance training resulted in significant decrease in fasting glucose ( P -value< 0.0001) and decrease in serum insulin ( P -value< 0.0001). However, no significant difference was found in FOXO1 expression in liver cells between exercise and control groups ( P -value: 0.725). Conclusion: Based on this data, improvement of glycemic profile in response to resistance training in diabetic rats cannot be attributed to FOXO1 expression in liver cells. This improvement may be attributed to an increase in serum insulin or other hormonal or genetic changes in response to resistance exercise, which will require further studies in this area.
Effect of Interval and Continued Exercises with Crocin on Bax/Bcl-2 in Diabetic Obese Rats
Authors: Hassanpour Ghobad , Nikbakht Hojatollah , Azarbayjani Mohammad Ali , Shakeri Nader , Abed Natanzi Hossein
Published: April 2020

Objective: Diabetes is a metabolic disease which is linked to increased physical disabilities and muscle tissue damage. The aim of this study was to investigate the effect of interval and continued exercises with crocin on Bax/Bcl-2 ratio in diabetic obese rats. Materials and Methods: In this clinical trial study, 56 adult diabetic rats (high-fat diet and venous injection of streptozotocin) were selected and randomly assigned to groups (1), intense interval exercises (2), low intensity exercise (3), intense interval exercise with crocin consumption, (4) Low intensity exercise with crocin consumption, (5) Crocin consumption, (6) sham and (7) control were divided. Intense interval and low intensity exercise groups lasted for 8 weeks, three sessions per week, with intensity of 80 to 85 and 50 to 55 percent of maximum treadmill running, and crocin consumption groups for 8 weeks per day, mg / kg 25 crocin were taken peritoneal. To analyze the research hypotheses, Kolmogorov- Smirnov tests, independent T- tests and two-way multi-variable analysis of variance were used along with Benferron's comparison method. It should be noted that the significance level in all measurements was considered to be P -value≤ 0.05. Results: Results showed that exercise ( P -value: 0.12) and crocin ( P value: 0.10) consumption had no significant effect on Bax/Bcl-2 gene expression in diabetic rats. Also interaction of exercise and crocin consumption on Bax/Bcl-2 was not significant ( P -value: 0.12). Conclusion: It appears that exercise and crocin consumption have not interaction effect on improvement of Bax / Bcl-2 ratio in diabetic rats.
Investigation Trp64Arg polymorphism of the beta 3-adrenergic receptor gene in nonobese women with polycystic ovarian syndrome
Authors: Zafari Zangeneh Farideh , Sarmast Shoushtari Maryam , Shojaee Sahar , Aboutorabi Elahe
Published: April 2020

Background: Polycystic ovary syndrome (PCOS) is a multifactorial and heterogeneous disease that has a potent inheritable component based on familial clustering. Despite many studies in the genetic field of PCOS, the genes that are involved in the causes of this syndrome have not been thoroughly investigated.
Objective: The purpose of this study was to establish the occurrence of the Trp64Arg polymorphism of beta3 adrenergic receptor in non-obese women with PCOS.
Materials and Methods: This cross-sectional study was performed on 100 women with PCOS and normal women as the control group in Imam Khomeini Hospital of Tehran in 2016-2017. Peripheral blood sample (2 cc) was obtained from two groups for genomic DNA based on the gene bank. Polymorphisms were genotyped by of using ADRB3 Trp64Arg. Then the DNA was extracted by genomic kiagen kit. The primer was analyzed for PCR based on gene bank by using Primer3 software and then confirmed by primer Blast tool at NCBI site to conformity to the beta-3 adrenergic receptor gene. The protein changes were assessment by the Clastal W software.
Results: The sequence analysis presented in NCBI, transcript variant 1, with the code NM_000025.2, shows changes in the amino acid sequence of exon 1 in women with PCOS. Polymorphism in the codon 64 encoding the amino acid tryptophan (W) occurred in the nucleotide c.T190C, which changed the nucleotide T to C and then the amino acid sequence of the tryptophan was altered to arginine pW64R.
Conclusion: T-C polymorphism is evident in the codon 64 of the adrenergic β3 receptor in patients with PCOS. Therefore, Beta3 adrenergic receptor gene polymorphism (Thr164Ile) associates with this syndrome in nonobese women.
Key words: Codon 64, Beta-3 adrenergic receptor, Polymorphism, Polycystic ovarian syndrome.
Is psychosexual therapy a reliable alternative to bupropion extended-release to promote the sexual function in infertile women? An RCT
Authors: Pasha Hajar , Basirat Zahra , Faramarzi Mahbobeh , Kheirkhah Farzan
Published: April 2020

Background: Various treatment methods are used to deal with sexual problems.
Objective: This study was applied to answer the question of whether psychosexual therapy (PST) can be a reliable alternative to bupropion extended-release (BUP ER) to promote sexual function in infertile women.
Materials and Methods: In this randomized clinical trial, 105 infertile women with sexual dysfunction were randomly allocated to three groups: PST, BUP ER, and a control group. The PST group participated in a total of eight 2-hr group sessions. In BUP ER group, 150 mg/day Bupropion ER was administered for eight weeks. The control group did not receive any interventions. The female sexual function index (FSFI) and a clinical interview were used to assess their sexual dysfunction.
Results: The mean pre-to-post treatment scores of FSFI and its subscales increased significantly in PST and BUP ER groups (except in the subscale of sexual pain) (p = 0.0001, p = 0.0001). The changes in the subjects were not significant in the control group. After adjusting for the baseline values, the results remained significant for the mean FSFI (p = 0.0001), and its subscales between the groups. Compared to the control group, a significant increase was observed in the mean FSFI (p = 0.0001, p = 0.002) and its subscales in the PST group and in the BUP ER group (except in the subscale of sexual pain). Comparison of two intervention methods showed that PST had the better effect on the sexual function improving (p = 0.0001) and its subscales (exempting the subscale of orgasm) than BUP ER.
Conclusion: PST can be considered not only a reliable alternative to pharmacotherapy; it also produces better results in terms of improving sexual function in infertile women.
Key words: Infertility, Sexual activities, Drug therapy, Psychotherapy, Bupropion.
Effects of clomiphene citrate plus estradiol or progesterone on endometrial ultrastructure: An RCT
Authors: Taheripanah Robabeh , Kabir-Salmani Maryam , Favayedi Masoomeh , Zamaniyan Marzieh , Malih Narges , Taheripanah Anahita
Published: April 2020

Background: Pinopods concentrations in endometrial surface is a marker of implantation. Estradiol valerate (EV) was used to change the adverse effects of Clomiphene Citrate (CC) on the endometrium.
Objective: The goal was to assess whether there is a significant difference in the endometrial pinopods concentrations and other parameters after adding EV and progesterone to higher doses of CC.
Materials and Methods: In this prospective randomized clinical trial, a total of 30 women who did not respond to 100 mg of CC from February 2016 to June 2016 were evaluated. They were divided into three groups: group I) received 150 mg of CC alone, group II) CC with EV, and group III) CC plus progesterone. On day 21 of the menstrual cycle, endometrial biopsy, a blood sampling, and a scanning by electron microscopy were performed.
Results: On day 21 of the menstrual cycle, there was no significant difference in the pinopods concentrations (p = 0.641) and serum estrogen levels (p = 0.276) between groups. However, the Serum progesterone levels in group I was higher than the other two groups (p = 0.007) in the same day.
Conclusion: Since the addition of EV and progesterone to higher dosages of CC did not change the pinopods concentration and serum estrogen levels on day 21 of the menstrual cycle, and the serum progesterone levels was higher in CC alone group (i.e. group I) compared to other groups, it can be concluded that the anti-estrogenic effects of CC just appear on the endometrium and not on the plasma levels.
Key words: Ovulation induction, Clomiphene, Estradiol, Progesterone, Electron microscopy, Endometrium.
Status of sickness absence in the workers of a home appliance manufacturing company
Authors: Hosseininejad Mahin , Mohammadi Saber , Mirzamohammadi Elham , Kabir-mokamelkhah Elahe
Published: April 2020

Introduction: In recent years, sickness absence in industrialized societies has been accompanied by a lot of costs and problems and is still increasing. In addition, sickness absence is progressively used as a health indicator among the workforce. The aim of this study was to determine and compare the status of absenteeism due to illness in workers of a home appliance company.Methods: This cross-sectional study was conducted on workers of a home appliance manufacturing company from September 2015 till September 2016. Information about the number of days and episodes of sickness absence and personal information was collected.Results: The sickness absence in blue-collar worker was higher than the white-collar groups (OR=2.20, and P-value < 0.001). In addition, the total and long-term absence in shift workers was higher than non-shift workers. Also, older age and higher body mass index was associated with lower sickness absence. Long-term sickness absence was more common among smokers than non-smokers.Conclusion: Findings of this study show that sickness absence among workers can be affected by various demographic and occupational factors including age, body mass index, smoking status, and work group.
HEART assessment of human error by the hospital operating rooms staff Imam Hussein (AS) and provide solutions Golpayegan city prioritized
Authors: Asgari Mahsa , Halvani Gholamhosein , Mehrparvar Amirhooshang , Fallah Hossein , Zarinkafsh Majid
Published: April 2020

Considering the importance of medical care and especially occupations in the operating room for the improvement of illnesses as well as the preservation of life, the consequences of failure and failure to perform the duties of the staff of these centers will be irreparable, so the findings of this study can be a useful guide to reduce Errors in operating room staff.Methods: This study was performed by HEART method to investigate the human error in the operating room staff. To perform this study, presenting various shifts in the operating rooms, training and justifying the employees of this department regarding the study and its objectives, the staffing process The operating room was observed in various occupations and the existing documents and guidelines were reviewed. Also, the main steps, including hierarchical analysis of occupations, identifying errors in terms of each task and sub task, completing other parts of the work sheet and determining the probability of human error and providing Prioritized control strategies were carried out in the next steps.Results: According to the results, Fatigue factor with frequency of 164 (11.47%) and negligence with frequency of 160 (11.2%) were the most frequent errors in the occupations of operation rooms of this hospital. The inadequate work environment with frequency 1 (/0007%) The least factor is the error.Conclusion: equipping the operating rooms with advanced equipment, employing and recruiting skilled and experienced personnel, adjusting the work schedule and resting the staff, providing training tailored to the needs of the staff, and improving the supervisory and management systems. The most important ways to improve existing conditions and reduce Human errors
Prevalence of musculoskeletal disorders and determination of associated risk factors in female workers in clothing manufacturing workshops of Yazd city
Authors: Neshastegar Fateme , Halvani Gholamhossein , Choobineh Alireza , AhmadiyeYazdi Mohammadhossein
Published: April 2020

Introduction: Upper limb musculoskeletal disorders are prevalent and undesirable health consequences among workers in clothing industry. To improve working conditions, workplace ergonomic assessment is necessary. This study was conducted among workers of clothing manufacturing workshops to determine the prevalence of musculoskeletal disorders and to assess ergonomic working conditions to identify major risk factors associated with musculoskeletal symptoms.Methods: One hundred and fifty female workers from clothing manufacturing workshops of Yazd city participated. The Nordic questionnaire and an ergonomics checklist consisting of 6 sections were used as data collection tools. An index was calculated for each section of the checklist. Action categories indicating the priority of corrective measures were also defined. Data analysis was performed using the ANOVA and ROC curve tests by SPSS software (version 17).Results: The results showed a high prevalence of musculoskeletal disorders, especially in the upper limbs among the study population. In this study, a significant association was found between the calculated ergonomics index and MSDs in different body regions (p<0.05). The mean of this index among the workers who reported symptoms was less than those of the other group indicating undesirable ergonomic conditions. In addition, 68.7% of the workers were categorized in the first priority of intervention (requiring ergonomic intervention as soon as possible).Conclusion: The prevalence of MSDs was high among the workers of clothing workshops. The developed checklist was considered as an appropriate and low cost tool for ergonomic evaluation of sewing workshops. The results also indicated that most ergonomic problems in these workshops originate from inappropriate general working conditions, work organization, manual material handling, hand tools, workstation and working post
Evaluating and managing the probability of medical errors in nursing personnel using the HEART method
Authors: Mirza Beigi H , Halvani GH , Zare S , Anooshe V , Jam bar sang S , Soltani NA
Published: April 2020

Introduction: Medical errors cause serious and often preventable injuries to patients. Studying human errors and their use as an opportunity for learning is a key factor in the effort to improve patient safety and quality of care in the hospitals. The purpose of this study was to identify and evaluate human errors to reduce their risks in nursing personnel using the Human Error Evaluation and Reduction (HEART) method.Materials and Methods: This cross-sectional descriptive study was performed in one of the hospitals of Rafsanjan in 1977. Initially, hierarchical task analysis was performed using HTA method and nurses' tasks were identified. In the next step, the probability of their human errors during work for all tasks was determined using the HEART method and the factors and conditions that were effective in causing the error were determined. Finally, in light of these conditions, suggestions were made to reduce the likelihood of these errors occurring.Results: The mean (standard deviation) human error score for the different tasks of nursing job was 1.79 (3.44). The maximum likelihood of error occurring in relation to the task of regulating and applying DC shock in emergencies was determined as 14.48. The least probability of error occurring for skin care task was 0.0014. The results of this study regarding the conditions that can lead to human error in nurses showed that the most important error generating conditions in nurses were novice and inexperienced, high stress, unreliable work tool, lack of control system transparency, lack of clarity and timely and direct confirmation of action. The highest mean human error probability score for Type C tasks was 4.34. Also the mean human error probability score for D, E and G tasks were 0.225, 0.198, and 0.00206 from right to left, respectively. The results of Kruskal-Wallis nonparametric test showed that there was a statistically significant difference among the mean scores of human error probability scores of different groups (P-value <0.001).Conclusion: Considering the critical consequences of human errors in nurses' work activities, developing specialized guidelines for their various activities, providing specialized training, as well as retraining of scientific and practical skills, increasing the workforce, reducing overtime, regular work shifts to improve the reliability of medical staff performance and familiarize staff with the risks that may result from errors in patients are necessary to reduce the likelihood of errors occurring in nurses.
National Referendum as an Example for Legitimizing Authoritarian Rule (the Case of the Republic of Belarus after 1991)
Authors: Ziętara Wojciech
Published: April 2020

The article is the result of research project no. 2014/15/B/HS5/01866 funded by the National Science Centre.