bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrating Artificial Intelligence for Academic Advanced Therapy Medicinal Products: Challenges and Opportunities
Authors: Aguilar-Gallardo Cristobal , Bonora-Centelles Ana
Published: February 2024

Cell and gene therapies represent promising new treatment options for many diseases, but also face challenges for clinical translation and delivery. Hospital-based GMP facilities enable rapid bench-to-bedside development and patient access but require significant adaptation to implement pharmaceutical manufacturing in healthcare infrastructures constrained by space, regulations, and resources. This article reviews key considerations, constraints, and solutions for establishing hospital facilities for advanced therapy medicinal products (ATMPs). Technologies like process analytical technology (PAT), continuous manufacturing, and artificial intelligence (AI) can aid these facilities through enhanced process monitoring, control, and automation. However, quality systems tailored for product quality rather than just compliance, and substantial investment in infrastructure, equipment, personnel, and multi-departmental coordination, remain crucial for successful hospital ATMP facilities and to drive new therapies from research to clinical impact.
Optimization of Pin Type Single Screw Mixer for Fabrication of Functionally Graded Materials
Authors: Wang Shijie , Zhou Jing , Duan Guolin
Published: February 2024

The direct ink writing (DIW) process, used for creating components with functionally graded materials, holds significant promise for advancement in various advanced fields. However, challenges persist in achieving complex gradient variations in small-sized parts. In this study, we have developed a customized pin shape for an active screw mixer using a combination of quadratic B-Spline, the response surface method, and global optimization. This tailored pin design was implemented in a two-material extrusion-based printing system. The primary objective is to facilitate the transformation of material components with shorter transition distances, overcoming size constraints and enhancing both printing flexibility and resolution. Moreover, we characterized the transition delay time for material component changes and the mixing uniformity of the extruded material by constructing a finite element simulation model based on computational fluid dynamics. Additionally, we employed a particle tracking method to obtain the Lyapunov exponent and Poincaré map of the mixing process. We employed these metrics to represent and compare the degree of chaotic mixing and dispersive mixing ability with two other structurally similar mixers. It was found that the optimized pin-type mixer can reduce the transition delay distance by approximately 30% compared to similar structures. Finally, comparative experiments were carried out to verify the printing performance of the optimized pin-type active mixer and the accuracy of the finite element model.
Changes in the Mechanical, Sensory, and Microbiological Properties during the Storage of Innovative Vegetable and Meat Soups for Seniors
Authors: Stangierski Jerzy , Kawecka Agata , Rezler Ryszard , Tomczyk Łukasz , Siejak Przemysław
Published: February 2024

This study was conducted on vegetable soup with rabbit meat and vegetable soup with rabbit meat, beef balls, and carrots. The qualitative characteristics of the soups were adapted to the needs of elderly consumers. The soups used in the experiments were industrially produced. The aim of this study was to analyse changes in the mechanical, sensory, and microbiological properties of the soups occurring during their storage (1, 7, 14, and 21 days). Strength tests were performed at temperatures of 20 °C and 55 °C. Both soups had a high protein content (4.7–6.5%), low sugar (0.3–0.5%) and salt content (0.8%), and a fibre content of 1.4%. The texture analysis showed great similarity in the mechanical characteristics of both soups. The samples were characterised by low measured values for firmness (0.72 N) and cohesiveness (−0.14 N) in both temperatures. The average shear force of the beef balls with carrots at 20 °C was 12.3 N, but after heating, it decreased to 8.8 N (p < 0.05). The rheological tests on the soups showed that they were characterised by a relatively high viscosity (15–20 Pas at 55 °C). Storage of the soups for 21 days did not significantly affect their rheological parameters (p > 0.05). The soup with beef balls and carrots was rated higher by the sensory panel. On the 21st day of storage, the permitted limit of the count of bacteria was not exceeded in either of the samples. This study shows that the soups had desirable structural, nutritional, and sensory characteristics, which are important for this group of consumers. The values of the mechanical parameters of all the samples were low, and they were even significantly more reduced when the products were heated. This may suggest that the products should not be difficult to consume for seniors.
New Conditions for Testing the Oscillation of Solutions of Second-Order Nonlinear Differential Equations with Damped Term
Authors: Al-Jaser Asma , Qaraad Belgees , Ramos Higinio , Serra-Capizzano Stefano
Published: February 2024

This paper deals with the oscillatory behavior of solutions of a new class of second-order nonlinear differential equations. In contrast to most of the previous results in the literature, we establish some new criteria that guarantee the oscillation of all solutions of the studied equation without additional restrictions. Our approach improves the standard integral averaging technique to obtain simpler oscillation theorems for new classes of nonlinear differential equations. Two examples are presented to illustrate the importance of our findings.
Tractability of Approximation of Functions Defined over Weighted Hilbert Spaces
Authors: Yan Huichao , Chen Jia
Published: February 2024

We investigate L2-approximation problems in the worst case setting in the weighted Hilbert spaces H(KRd,α,γ) with weights Rd,α,γ under parameters 1≥γ1≥γ2≥⋯≥0 and 1<α1≤α2≤⋯. Several interesting weighted Hilbert spaces H(KRd,α,γ) appear in this paper. We consider the worst case error of algorithms that use finitely many arbitrary continuous linear functionals. We discuss tractability of L2-approximation problems for the involved Hilbert spaces, which describes how the information complexity depends on d and ε−1. As a consequence we study the strongly polynomial tractability (SPT), polynomial tractability (PT), weak tractability (WT), and (t1,t2)-weak tractability ((t1,t2)-WT) for all t1>1 and t2>0 in terms of the introduced weights under the absolute error criterion or the normalized error criterion.
C-S and Strongly C-S Orthogonal Matrices
Authors: Liu Xiaoji , Liu Ying , Jin Hongwei
Published: February 2024

In this paper, we present a new concept of the generalized core orthogonality (called the C-S orthogonality) for two generalized core invertible matrices A and B. A is said to be C-S orthogonal to B if ASB=0 and BAS=0, where AS is the generalized core inverse of A. The characterizations of C-S orthogonal matrices and the C-S additivity are also provided. And, the connection between the C-S orthogonality and C-S partial order has been given using their canonical form. Moreover, the concept of the strongly C-S orthogonality is defined and characterized.
Phosphorus-Containing Polymer Electrolytes for Li Batteries
Authors: Varan Narcis , Merghes Petru , Plesu Nicoleta , Macarie Lavinia , Ilia Gheorghe , Simulescu Vasile
Published: February 2024

Lithium-ion polymer batteries, also known as lithium-polymer, abbreviated Li-po, are one of the main research topics nowadays in the field of energy storage. This review focuses on the use of the phosphorus containing compounds in Li-po batteries, such as polyphosphonates and polyphosphazenes. Li-po batteries are mini-devices, capable of providing power for any portable gadget. From a constructive point of view, Li-po batteries contain an anode (carbon), a cathode (metal oxide), and a polymer electrolyte, which could be liquid electrolytes or solid electrolytes. In general, a divider is used to keep the anode and cathode from touching each other directly. Since liquid electrolytes have a generally high ionic conductivity, they are frequently employed in Li-ion batteries. In the last decade, the research in this field has also focused on solving safety issues, such as the leakage of electrolytes and risk of ignition due to volatile and flammable organic solvents. The research topics in the field of Li-po remain focused on solving safety problems and improving performance.
On the State of Usability for Lithium–Ion Batteries
Authors: Wett Christopher , Lampe Jörg , Haß Jan , Seeger Thomas , Turan Bugra
Published: February 2024

Lithium–ion batteries are well established as traction batteries for electric vehicles. This has led to a growing market for second-life batteries that can be used in applications like home energy storage systems. Moreover, the recyclability and safe handling of aged or damaged cells and packs has become more important. While there are several indicators, like state of health (SOH), state of power (SOP), or state of safety (SOS), which describe the state of a battery before its defined end of life (EOL), there is no consistent classification methodology by which to describe the usability of a cell or pack after its EOL is reached. The proposed state of usability (SOU) provides a new indicator that accounts for the usability for second life, recyclability, and possible required safety handling of a lithium–ion battery after its first intended life cycle. This work presents a decision tree method, which in turn leads to five discrete usability levels enabling a fast and rough determination of the SOU for practical use. Further, a calculation methodology for reasonable continuous regions of the SOU is proposed. Both methods are based on a literature-based rating of all of the relevant defect and aging mechanisms displayed in a risk matrix. Finally, some experimental methods that can be used for SOU determination are proposed. The developed methodology and the hands-on approach using a decision tree are well-suited for real world application in recycling companies and battery test laboratories.
Mechanical Behavior of Polyurethane Insulation of CRT Leads in Cardiac Implantable Electronic Devices: A Comparative Analysis of In Vivo Exposure and Residual Properties
Authors: Salih Anmar , Goswami Tarun
Published: February 2024

Left ventricle leads are designed for the purpose of long-term pacing in the left ventricle. This study investigated the leads that use polyurethane as an outer insulator and SI-polyimide as an inner insulator. Polyurethane is commonly used for the outer insulation of cardiac leads due to its flexibility and biocompatibility. SI-polyimide (SI-PI) is a high-performance material known for its electrical insulation properties and is used for the inner insulation to maintain the integrity of the electrical pathways within the lead. Ten leads were received from the Wright State University Anatomical Gift Program. The duration of in vivo implantation varied for each lead, from less than a month to 108 months, with an average in vivo duration of 41 ± 31 months. We used the Test Resources Q series system for conducting our tests, as well as samples prepared to ensure compliance with the ASTM Standard D 1708-02a and the ASTM Standard D 412-06a. During the test, the load was applied to the intact lead. Before conducting individual tests, each lead was carefully inspected for surface defects. After conducting the tests, the load to failure, percentage of elongation, percentage of elongation at 5 N, ultimate tensile strength, and modulus of elasticity were calculated. There was no significant difference in load to failure, the percentage of elongation to failure, ultimate tensile strength, and modulus of elasticity (p-value = 0.82, p-value = 0.62, p-value = 0.82, and p-value = 0.12), respectively, when compared to in vivo exposure time. On the other hand, the percentage of elongation at 5 N force showed a significant difference (p-value = 0.0066) after 60 months in an in vivo environment. As the duration of in vivo exposure increased, the load to failure, percentage of elongation, ultimate tensile strength, and modulus of elasticity decreased insignificantly. The residual properties of these left ventricle leads remained relatively stable after 108 months of in vivo exposure duration, with no statistically significant degradation or changes in performance.
Integrated Analysis of Gut Microbiome and Adipose Transcriptome Reveals Beneficial Effects of Resistant Dextrin from Wheat Starch on Insulin Resistance in Kunming Mice
Authors: Chen Xinyang , Hou Yinchen , Liao Aimei , Pan Long , Yang Shengru , Liu Yingying , Wang Jingjing , Xue Yingchun , Zhang Mingyi , Zhu Zhitong , Huang Jihong
Published: February 2024

Systemic chronic inflammation is recognized as a significant contributor to the development of obesity-related insulin resistance. Previous studies have revealed the physiological benefits of resistant dextrin (RD), including obesity reduction, lower fasting glucose levels, and anti-inflammation. The present study investigated the effects of RD intervention on insulin resistance (IR) in Kunming mice, expounding the mechanisms through the gut microbiome and transcriptome of white adipose. In this eight-week study, we investigated changes in tissue weight, glucose–lipid metabolism levels, serum inflammation levels, and lesions of epididymal white adipose tissue (eWAT) evaluated via Hematoxylin and Eosin (H&E) staining. Moreover, we analyzed the gut microbiota composition and transcriptome of eWAT to assess the potential protective effects of RD intervention. Compared with a high-fat, high-sugar diet (HFHSD) group, the RD intervention significantly enhanced glucose homeostasis (e.g., AUC-OGTT, HOMA-IR, p < 0.001), and reduced lipid metabolism (e.g., TG, LDL-C, p < 0.001) and serum inflammation levels (e.g., IL-1β, IL-6, p < 0.001). The RD intervention also led to changes in the gut microbiota composition, with an increase in the abundance of probiotics (e.g., Parabacteroides, Faecalibaculum, and Muribaculum, p < 0.05) and a decrease in harmful bacteria (Colidextribacter, p < 0.05). Moreover, the RD intervention had a noticeable effect on the gene transcription profile of eWAT, and KEGG enrichment analysis revealed that differential genes were enriched in PI3K/AKT, AMPK, in glucose-lipid metabolism, and in the regulation of lipolysis in adipocytes signaling pathways. The findings demonstrated that RD not only ameliorated IR, but also remodeled the gut microbiota and modified the transcriptome profile of eWAT.
Brain-Derived Neurotrophic Factor in Pediatric Acquired Brain Injury and Recovery
Authors: Treble-Barna Amery , Petersen Bailey A. , Stec Zachary , Conley Yvette P. , Fink Ericka L. , Kochanek Patrick M.
Published: February 2024

We review emerging preclinical and clinical evidence regarding brain-derived neurotrophic factor (BDNF) protein, genotype, and DNA methylation (DNAm) as biomarkers of outcomes in three important etiologies of pediatric acquired brain injury (ABI), traumatic brain injury, global cerebral ischemia, and stroke. We also summarize evidence suggesting that BDNF is (1) involved in the biological embedding of the psychosocial environment, (2) responsive to rehabilitative therapies, and (3) potentially modifiable. BDNF’s unique potential as a biomarker of neuroplasticity and neural repair that is reflective of and responsive to both pre- and post-injury environmental influences separates it from traditional protein biomarkers of structural brain injury with exciting potential to advance pediatric ABI management by increasing the accuracy of prognostic tools and informing clinical decision making through the monitoring of therapeutic effects.
Intermediate Repeat Expansion in the ATXN2 Gene as a Risk Factor in the ALS and FTD Spanish Population
Authors: Borrego-Hernández Daniel , Vázquez-Costa Juan Francisco , Domínguez-Rubio Raúl , Expósito-Blázquez Laura , Aller Elena , Padró-Miquel Ariadna , García-Casanova Pilar , Colomina María J. , Martín-Arriscado Cristina , Osta Rosario , Cordero-Vázquez Pilar , Esteban-Pérez Jesús , Povedano-Panadés Mónica , García-Redondo Alberto
Published: February 2024

Intermediate CAG expansions in the gene ataxin-2 (ATXN2) are a known risk factor for ALS, but little is known about their role in FTD risk. Moreover, their contribution to the risk and phenotype of patients might vary in populations with different genetic backgrounds. The aim of this study was to assess the relationship of intermediate CAG expansions in ATXN2 with the risk and phenotype of ALS and FTD in the Spanish population. Repeat-primed PCR was performed in 620 ALS and 137 FTD patients in three referral centers in Spain to determine the exact number of CAG repeats. In our cohort, ≥27 CAG repeats in ATXN2 were associated with a higher risk of developing ALS (odds ratio [OR] = 2.666 [1.471–4.882]; p = 0.0013) but not FTD (odds ratio [OR] = 1.446 [0.558–3.574]; p = 0.44). Moreover, ALS patients with ≥27 CAG repeats in ATXN2 showed a shorter survival rate compared to those with <27 repeats (hazard ratio [HR] 1.74 [1.18, 2.56], p = 0.005), more frequent limb onset (odds ratio [OR] = 2.34 [1.093–4.936]; p = 0.028) and a family history of ALS (odds ratio [OR] = 2.538 [1.375–4.634]; p = 0.002). Intermediate CAG expansions of ≥27 repeats in ATXN2 are associated with ALS risk but not with FTD in the Spanish population. ALS patients carrying an intermediate expansion in ATXN2 show more frequent limb onset but a worse prognosis than those without expansions. In patients carrying C9orf72 expansions, the intermediate ATXN2 expansion might increase the penetrance and modify the phenotype.
Prediction of Non-Muscle Invasive Papillary Urothelial Carcinoma Relapse from Hematoxylin–Eosin Images Using Deep Multiple Instance Learning in Patients Treated with Bacille Calmette–Guérin Immunotherapy
Authors: Drachneris Julius , Morkunas Mindaugas , Fabijonavicius Mantas , Cekauskas Albertas , Jankevicius Feliksas , Laurinavicius Arvydas
Published: February 2024

The limited reproducibility of the grading of non-muscle invasive papillary urothelial carcinoma (NMIPUC) necessitates the search for more robust image-based predictive factors. In a cohort of 157 NMIPUC patients treated with Bacille Calmette–Guérin (BCG) immunotherapy, we explored the multiple instance learning (MIL)-based classification approach for the prediction of 2-year and 5-year relapse-free survival and the multiple instance survival learning (MISL) framework for survival regression. We used features extracted from image patches sampled from whole slide images of hematoxylin–eosin-stained transurethral resection (TUR) NPMIPUC specimens and tested several patch sampling and feature extraction network variations to optimize the model performance. We selected the model showing the best patient survival stratification for further testing in the context of clinical and pathological variables. MISL with the multiresolution patch sampling technique achieved the best patient risk stratification (concordance index = 0.574, p = 0.010), followed by a 2-year MIL classification. The best-selected model revealed an independent prognostic value in the context of other clinical and pathologic variables (tumor stage, grade, and presence of tumor on the repeated TUR) with statistically significant patient risk stratification. Our findings suggest that MISL-based predictions can improve NMIPUC patient risk stratification, while validation studies are needed to test the generalizability of our models.
The Han:SPRD Rat: A Preclinical Model of Polycystic Kidney Disease
Authors: Kofotolios Ioannis , Bonios Michael J. , Adamopoulos Markos , Mourouzis Iordanis , Filippatos Gerasimos , Boletis John N. , Marinaki Smaragdi , Mavroidis Manolis
Published: February 2024

Autosomal Dominant Polycystic Kidney Disease (ADPKD) stands as the most prevalent hereditary renal disorder in humans, ultimately culminating in end-stage kidney disease. Animal models carrying mutations associated with polycystic kidney disease have played an important role in the advancement of ADPKD research. The Han:SPRD rat model, carrying an R823W mutation in the Anks6 gene, is characterized by cyst formation and kidney enlargement. The mutated protein, named Samcystin, is localized in cilia of tubular epithelial cells and seems to be involved in cystogenesis. The homozygous Anks6 mutation leads to end-stage renal disease and death, making it a critical factor in kidney development and function. This review explores the utility of the Han:SPRD rat model, highlighting its phenotypic similarity to human ADPKD. Specifically, we discuss its role in preclinical trials and its importance for investigating the pathogenesis of the disease and developing new therapeutic approaches.
Transcriptome-Wide N6-Methyladenosine Alternations in Pulmonary Arteries of Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats and Novel Therapeutic Targets
Authors: Feng Yilu , Yu Zaixin , Tang Mi , Li Jiang , Peng Baohua , Juaiti Mukamengjiang , Tang Yiyang , Liang Benhui , Ouyang Mingqi , Liu Qingqing , Song Jie
Published: February 2024

N6-methyladenosine (m6A) is a post-transcriptional epigenetic change with transcriptional stability and functionality regulated by specific m6A-modifying enzymes. However, the significance of genes modified by m6A and enzymes specific to m6A regulation in the context of pulmonary arterial hypertension (PAH) remains largely unexplored. MeRIP-seq and RNA-seq were applied to explore variances in m6A and RNA expression within the pulmonary artery tissues of control and monocrotaline-induced PAH rats. Functional enrichments were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. To screen candidate m6A-related genes, the STRING and Metascape databases were used to construct a protein–protein interaction network followed by a real-time PCR validation of their expression. The expression level of an m6A regulator was further investigated using immunohistochemical staining, immunofluorescence, and Western blot techniques. Additionally, proliferation assays were conducted on primary rat pulmonary artery smooth muscle cells (PASMCs). We identified forty-two differentially expressed genes that exhibited either hypermethylated or hypomethylated m6A. These genes are predominantly related to the extracellular matrix structure, MAPK, and PI3K/AKT pathways. A candidate gene, centromere protein F (CENPF), was detected with increased expression in the PAH group. Additionally, we first identified an m6A reader, leucine rich pentatricopeptide repeat containing (LRPPRC), which was downregulated in the PAH rat model. The in vitro downregulation of Lrpprc mediated by siRNA resulted in the enhanced proliferation and elevated expression of Cenpf mRNA in primary rat PASMCs. Our study revealed a modified transcriptome-wide m6A landscape and associated regulatory mechanisms in the pulmonary arteries of PAH rats, potentially offering a novel target for therapeutic strategies in the future.
Plant Monoterpenes and Essential Oils as Potential Anti-Ageing Agents: Insights from Preclinical Data
Authors: Zuzarte Mónica , Sousa Cátia , Alves-Silva Jorge , Salgueiro Lígia
Published: February 2024

Ageing is a natural process characterized by a time-dependent decline of physiological integrity that compromises functionality and inevitably leads to death. This decline is also quite relevant in major human pathologies, being a primary risk factor in neurodegenerative diseases, metabolic disorders, cardiovascular diseases and musculoskeletal disorders. Bearing this in mind, it is not surprising that research aiming at improving human health during this process has burst in the last decades. Importantly, major hallmarks of the ageing process and phenotype have been identified, this knowledge being quite relevant for future studies towards the identification of putative pharmaceutical targets, enabling the development of preventive/therapeutic strategies to improve health and longevity. In this context, aromatic plants have emerged as a source of potential bioactive volatile molecules, mainly monoterpenes, with many studies referring to their anti-ageing potential. Nevertheless, an integrated review on the current knowledge is lacking, with several research approaches studying isolated ageing hallmarks or referring to an overall anti-ageing effect, without depicting possible mechanisms of action. Herein, we aim to provide an updated systematization of the bioactive potential of volatile monoterpenes on recently proposed ageing hallmarks, and highlight the main mechanisms of action already identified, as well as possible chemical entity–activity relations. By gathering and categorizing the available scattered information, we also aim to identify important research gaps that could help pave the way for future research in the field.
Oxygen Saturation Increase in Ischemic Wound Tissues after Direct and Indirect Revascularization
Authors: Račytė Austėja , Pikturnaitė Gabija , Baltrūnas Tomas , Kalvaitis Evaldas , Vaitėnas Gediminas , Skrebūnas Arminas , Baltrūnienė Vaida , Ručinskas Kęstutis
Published: February 2024

Background: The primary approach for treating ischemic wounds is restoring oxygen supply to the ischemic region. While direct angiosomal revascularization is often associated with better post-operative wound healing and limb salvage, its superiority over non-angiosomal revascularization remains controversial. This study aimed to compare intraoperative tissue oxygen saturation changes in ischemic zones following either direct or indirect revascularization in below-the-knee arteries. Methods: This prospective observational study included patients undergoing direct and indirect below-the-knee endovascular revascularizations. Assignment to the groups was not randomized. Near-infrared spectroscopy was used to monitor rSO2 changes near the ischemic wounds intraoperatively. The changes were compared between the groups. Results: 15 patients (50%) underwent direct angiosomal revascularization, while an equal number of patients underwent indirect revascularization. Overall, a statistically significant increase in regional oxygen saturation was observed after revascularization (p = 0.001). No statistically significant difference was found between the direct and indirect revascularization groups (p = 0.619). Conclusions: This study revealed a minor difference in the oxygen saturation increase between the angiosomal and non-angiosomal revascularization groups. Such a finding indicates that the clinical significance of angiosomal revascularization is negligible and might be concealed by confounding factors, such as the vessel diameter and outflow impact on the restenosis rate.
National Development in the Use of Inhaled Corticosteroid Treatment in Chronic Obstructive Pulmonary Disease: Repeated Cross-Sectional Studies from 1998 to 2018
Authors: Klitgaard Allan , Ibsen Rikke , Lykkegaard Jesper , Hilberg Ole , Løkke Anders
Published: February 2024

Recommendations for the treatment of chronic obstructive pulmonary disease (COPD) have shifted towards a more restrictive use of inhaled corticosteroids (ICS). We aimed to identify the nationwide development over time in the use of ICS treatment in COPD. We conducted a register-based repeated cross-sectional study using Danish nationwide registers. On a yearly basis from 1998 to 2018, we included all patients in Denmark ≥ 40 years of age with an ICD-10 diagnosis of COPD (J44). Accumulated ICS use was calculated for each year based on redeemed prescriptions. Patients were divided into the following groups: No ICS, low-dose ICS, medium-dose ICS, or high-dose ICS. From 1998 to 2018, the yearly proportion of patients without ICS treatment increased (from 50.6% to 57.6%), the proportion of patients on low-dose ICS treatment increased (from 11.3% to 14.9%), and the proportion of patients on high-dose ICS treatment decreased (from 17.0% to 9.4%). We demonstrated a national reduction in the use of ICS treatment in COPD from 1998 to 2018, with an increase in the proportion of patients without ICS and on low-dose ICS treatment and a decrease in the proportion of patients on high-dose ICS treatment.
Comprehensive Study on Carbon-Coated Silver for Improved Tribo-Electrical and Wetting Performance
Authors: Alderete Bruno , Mücklich Frank , Suarez Sebastian
Published: February 2024

The rise in electrification has considerably increased the demand for high-efficiency and durable electrical contact materials. Carbon nanoparticles (CNP) are a promising coating material due to their intrinsic transport properties (thus minimizing the impact on conductivity), their proven solid lubricity (potentially improving tribological performance), and their hydrophobic wetting behavior (potentially providing atmospheric protection). In this study, carbon nanotube and nanohorn coatings are produced via electrophoretic deposition on silver-plated surfaces, followed by tribo-electrical and wetting characterization. The proposed coatings do not negatively affect the conductivity of the substrate, showing resistance values on par with the uncoated reference. Tribo-electrical characterization revealed that the coatings reduce adhesive wear during fretting tests while maintaining stable and constant electrical contact resistance. Furthermore, CNP-coated surfaces show a hydrophobic wetting behavior toward water, with graphite and carbon nanotube (CNT) coatings approaching super-hydrophobicity. Prolonged exposure to water droplets during sessile drop tests caused a reduction in contact angle (CA) measurement; however, CNT coatings’ CA reduction after five minutes was only approximately 5°. Accordingly, CNP (specifically CNT) coatings show auspicious results for their application as wear and atmospheric protective barriers in electrical contacts.
Shear Stress and Sub-Femtomolar Levels of Ligand Synergize to Activate ALK1 Signaling in Endothelial Cells
Authors: Cheng Ya-Wen , Anzell Anthony R. , Morosky Stefanie A. , Schwartze Tristin A. , Hinck Cynthia S. , Hinck Andrew P. , Roman Beth L. , Davidson Lance A.
Published: February 2024

Endothelial cells (ECs) respond to concurrent stimulation by biochemical factors and wall shear stress (SS) exerted by blood flow. Disruptions in flow-induced responses can result in remodeling issues and cardiovascular diseases, but the detailed mechanisms linking flow-mechanical cues and biochemical signaling remain unclear. Activin receptor-like kinase 1 (ALK1) integrates SS and ALK1-ligand cues in ECs; ALK1 mutations cause hereditary hemorrhagic telangiectasia (HHT), marked by arteriovenous malformation (AVM) development. However, the mechanistic underpinnings of ALK1 signaling modulation by fluid flow and the link to AVMs remain uncertain. We recorded EC responses under varying SS magnitudes and ALK1 ligand concentrations by assaying pSMAD1/5/9 nuclear localization using a custom multi-SS microfluidic device and a custom image analysis pipeline. We extended the previously reported synergy between SS and BMP9 to include BMP10 and BMP9/10. Moreover, we demonstrated that this synergy is effective even at extremely low SS magnitudes (0.4 dyn/cm2) and ALK1 ligand range (femtogram/mL). The synergistic response to ALK1 ligands and SS requires the kinase activity of ALK1. Moreover, ALK1’s basal activity and response to minimal ligand levels depend on endocytosis, distinct from cell–cell junctions, cytoskeleton-mediated mechanosensing, or cholesterol-enriched microdomains. However, an in-depth analysis of ALK1 receptor trafficking’s molecular mechanisms requires further investigation.
Comparative Analysis of the Comfort of Children and Adolescents in Digital and Conventional Full-Arch Impression Methods: A Crossover Randomized Trial
Authors: Serrano-Velasco Diego , Martín-Vacas Andrea , Cintora-López Patricia , Paz-Cortés Marta Macarena , Aragoneses Juan Manuel
Published: February 2024

The aim of this study was to evaluate the comfort of children and adolescents with conventional full-arch dental impression methods compared to two intraoral scanners (iTeroTM and PrimescanTM). Methods: A monocentric, analytical, controlled crossover study was designed to compare conventional impression and digital impression with two intraoral scanners (iTeroTM and PrimescanTM) in children and teenagers. Patient comfort was evaluated using a 100 mm VAS scale adapted to Spanish and for children. A descriptive and analytical statistical method was conducted with a confidence level of 95% (p ≤ 0.05) and asymptotic or bilateral significance. Results: A total of 51 subjects were enrolled in the study (mean age = 12.35 years). Although the group of 10–14-year-olds was the most numerous, gender was equally distributed among the age groups. None of the variables on the VAS scale showed differences between the gender categories (p > 0.05). There were differences (p < 0.05) with respect to the age categories, as the middle adolescent group showed the worst general perception and total comfort during the conventional impression. Statistically significant differences were found between all VAS scale items and the three impression methods (p < 0.05). Conclusions: The digital impression technique is superior in terms of total comfort to the conventional alginate impression in children and adolescents.
Theoretical–Methodological Foundations for the Global Integration Method (Método de Integração Global—MIG) in the Treatment of Autism Spectrum Disorder
Authors: Loffi Renato Guimarães , Cruz Thalita Karla Flores , Paiva Giulia Moreira , Souto Deisiane Oliveira , Barreto Simone Rosa , Santana Patrícia Aparecida Neves , Nascimento Amanda Aparecida Alves Cunha , Costa Fabiana Rachel Martins , Cota Elisa Braz , Haase Vitor Geraldi
Published: February 2024

Currently, there is no intervention model for autism spectrum disorder (ASD) that addresses all levels and factors of the International Classification of Functioning, Disability and Health (ICF, WHO). The most researched programs focus on naturalistic, developmental and behavioral approaches to socio-communication. Less attention has been paid to motor and environmental reactivity aspects (behavior/interest restriction and sensory reactivity). The evidence rationale for the Global Integration Method (MIG, “Método de Integração Global”), a model addressing sensorimotor reactivity in addition to socio-communication, is presented. MIG is an integrative, interdisciplinary, family-oriented intervention and naturalistic program that addresses all levels and moderating factors of ASD’s impact. MIG’s theoretical rationale is based on the predictive coding impairment and embodied cognition hypotheses. MIG incorporates both bottom-up (flexible therapeutic suit, social-motor synchronization) and top-down (schematic social information processing, narratives, imagery) strategies to promote the building and use of accurate, flexible and context-sensitive internal predictive models. MIG is based on the premises that predictive coding improves both socio-communication and environmental reactivity, and that the postural stabilization provided by the flexible therapeutic suit frees information processing resources for socio-cognitive learning. MIG builds on interdisciplinary, professionally and parentally mediated work based on behavioral principles of intensive training in a situated environment.
Participation Strategies of Parents of Children with Neurodevelopmental Disorders: An Exploratory Study
Authors: Shiozu Hiroyasu , Kimura Daisuke , Iwanaga Ryoichiro , Kurasawa Shigeki
Published: February 2024

Strategies are critical to promote child participation in important life activities. This study analyzed the participation strategies of the parents of children with neurodevelopmental disorders. Ninety-two Japanese elementary children with neurodevelopmental disorders and their parents were recruited. The parents completed the Participation and Environment Measure for Children and Youth (PEM-CY) questionnaire. Strategy text data obtained from the PEM-CY were analyzed with the co-occurrence network and correspondence analyses. The co-occurrence network analysis showed that the commonality of strategies to enable participation at home, school, and community settings was able to explain the child’s characteristics when involved in each setting. The correspondence analysis also suggested the need for specific strategies in each setting. The importance of strategies to improve the attitudinal environment and promote the participation of children with neurodevelopmental disorders was evident. Reducing stigma is important in all environments, especially in the public sphere. In addition, specific strategies are needed in each setting, suggesting the importance of context-specific approaches.
Risk for Recurrence in Long-Term Follow-Up of Children after Liver Transplantation for Hepatoblastoma or Hepatocellular Carcinoma
Authors: Stefanowicz Marek , Kaliciński Piotr , Ismail Hor , Kowalski Adam , Broniszczak Dorota , Szymczak Marek , Pankowska-Woźniak Katarzyna , Roszkiewicz Anna , Święszkowska Ewa , Kamińska Diana , Szymańska Sylwia , Kowalewski Grzegorz
Published: February 2024

The aim of this study was to assess the long-term results of liver transplantation (LT) in pediatric patients with unresectable hepatoblastoma (HB) or hepatocellular carcinoma (HCC) with special reference to the risk of tumor recurrence. We retrospectively analyzed data from 46 HB and 26 HCC patients who underwent LT between 1990 and 2022. In HCC patients, we compared outcomes depending on donor type. We evaluated the impact of a number of risk factors on recurrence-free survival after LT. Estimated patient survival after 5, 10, and 15 years was 82%, 73%, and 73% in the HB group and 79%, 75%, and 75% in the HCC group, respectively (p = 0.76). In the HCC group, living donor LT (LDLT) and deceased donor LT (DDLT) provided similar patient survival (p = 0.09). Estimated recurrence-free survival in patients who had three or fewer risk factors was significantly better than in patients with more than three risk factors (p = 0.0001). Adequate patient selection is necessary when considering LT for primary liver tumors in children. The presence of more than three risk factors is associated with a very high risk of recurrence and indicates poor prognosis, whereas extrahepatic disease may be considered a contraindication for transplantation.
Audiological Phenotypes of Connexin Gene Mutation Patterns: A Glance at Different GJB2/GJB6 Gene Mutation Profiles
Authors: Franz Leonardo , Incognito Alessandro , Gallo Chiara , Turolla Licia , Scquizzato Elisa , Cenedese Roberta , Matarazzo Alessandro , Savegnago Daniel , Zanatta Paolo , Genovese Elisabetta , de Filippis Cosimo , Marioni Gino
Published: February 2024

GJB2 mutations are the most common cause of autosomal-recessive non-syndromic sensorineural hearing loss (SNHL). The available evidence shows large phenotypic variability across different genotypes and allelic variants. The aim of this study was to investigate the clinical and audiological features of a cohort of subjects with different GJB2/GJB6 gene mutation profiles from a tertiary referral center in Northeastern Italy. We considered 57 patients with GJB2/GJB6 mutations presenting with congenital, non-syndromic SNHL, mainly coming from the Veneto region (Italy). The samples were screened for mutations in exons 1 and 2 of the GJB2 gene and for the GJB6 gene deletion del (GJB6-D13S1830). Free-field and air-conduction frequency-specific thresholds and the pure-tone average (PTA) were considered in the statistical analysis. Five patients (8.87%) had connexin gene mutations in simple heterozygosis, 15 (26.31%) in compound heterozygosis, 34 (59.64%) in homozygosis, and 3 (5.26%) with digenic patterns. The frequency-specific air-conduction thresholds showed significantly different mean values across the different genotypes (Roy’s largest-root test, p = 0.0473). Despite the evidence already available on genetic SNHL, many new insights are to be expected. Further large-scale prospective studies including different populations are necessary to confirm these preliminary findings about the clinical and audiological features of patients with different GJB2/GJB6 gene mutation patterns.
Non-Pharmacologic Intensive Interdisciplinary Pain Treatment in Pediatrics: Impact on Symptoms, Daily Functioning, and the Family Unit
Authors: Campanile Jessica , Wu Becky , Sonagra Maitry , McGill Mackenzie , Stryker Daneka , Bradford Jamie , Sherker Jennifer , Konieczny Tami , Sherry David D. , Gmuca Sabrina
Published: February 2024

Objectives: To assess non-pharmacologic treatment outcomes pertaining to health-related quality of life (HRQoL) in youth with chronic idiopathic pain and their families. Methods: We conducted a retrospective cohort study of 115 youth with chronic idiopathic pain enrolled in a non-pharmacologic, hospital-based intensive interdisciplinary pain treatment (IIPT) program. HRQoL measures for the patient (Pediatric Quality of Life Inventory [PedsQL] short form) and family unit (PedsQL Family Impact) were collected on admission and discharge as part of routine clinical care. Changes in PedsQL scores were calculated using the Wilcoxon signed-rank test. Multivariable linear regression was used to explore factors associated with patient-level HRQoL. Results: Both individuals and the family unit reported that their HRQoL improved in all domains by program completion. Improvements in pain and allodynia were present for program participants at the time of completion as well as at the 3-month follow-up, suggesting durability of these effects. Conclusions: A non-pharmacologic IIPT program is a compelling treatment for pediatric and adolescent chronic idiopathic pain, for both patients and the family unit. Patients participating in this program had positive treatment outcomes with significantly improved subjective and objective measures of physical, emotional, social, and cognitive function.
Relationship between Nutrition and Development of the Jaws in Children: A Pilot Study
Authors: Boo Gordillo Paula , Marqués Martínez Laura , Borrell García Carla , García Miralles Esther
Published: February 2024

Craniofacial growth and development have been shown to be influenced by various environmental factors that impact child development. This study aims to analyze the different patterns of feeding during early childhood, starting from birth, and assess the variability of nutrition during the first stage of childhood, along with the habits developed, to study their impact on jaw development. The study was conducted on a sample of twenty-five patients aged 3 to 5, following approval from the ethics committee of the Catholic University of Valencia. Informed consent was obtained from the fathers, mothers, and/or legal guardians, who were administered surveys on habits and diet. Cephalometric measurements within the parameters of ideal occlusion were subsequently taken. While previous studies examined this subject, the findings are challenging to evaluate. However, this study identified significant associations (p = 0.001) between clinical measurements and children’s eating habits. The growth and development of the craniofacial cavity are influenced by multiple factors, including a child’s diet and habits. Nonetheless, further research is required to determine whether diet can be considered a determining factor in proper jaw growth.
Comparison of Eosinophil Counts in Inflammatory Conditions: Multisystem Inflammatory Syndrome in Children, Kawasaki Disease, and Infectious Mononucleosis
Authors: Sarı Erdal , Erdede Özlem
Published: February 2024

This study examined the distinctions between multisystem inflammatory syndrome associated with coronavirus disease 2019, Kawasaki disease, and infectious mononucleosis. These three inflammatory disorders have commonalities according to clinical and laboratory results, particularly in relation to eosinophil levels. In this retrospective, single-center study, we documented the examination records (acute phase reactants and complete blood count) and clinical and cardiological findings of 130 patients diagnosed with multisystem inflammatory syndrome, Kawasaki disease, and infectious mononucleosis. These patients were treated and received follow-up care in our hospital from March 12, 2020, to September 13, 2022, as per the hospital records. Statistical analyses were performed using NCSS 2007, version 1 software. Eosinopenia was more prevalent in children with multisystem inflammatory syndrome than in those with Kawasaki disease, who showed normal or elevated eosinophil counts. The eosinophil counts in patients with infectious mononucleosis typically fell within the normal range. Our study found no correlation between the eosinophil counts and cardiac involvement in pediatric patients with either condition. These findings indicate a higher prevalence of eosinopenia in patients with multisystem inflammatory syndrome, irrespective of cardiac involvement, than in those with Kawasaki disease. Despite similarities in clinical findings, Kawasaki disease and multisystem inflammatory syndrome in children necessitate further studies for distinct characteristic elucidation.
Factors Influencing the Rheology of Methane Foam for Gas Mobility Control in High-Temperature, Proppant-Fractured Reservoirs
Authors: Parekh Aashish T. , Katiyar Amit , Nguyen Quoc P.
Published: February 2024

Gas-enhanced oil recovery (EOR) through huff-n-puff (HnP) is an important method of recovering oil from fracture-stimulated reservoirs. HnP productivity is hampered by fracture channeling, leading to early gas breakthroughs and gas losses. To mitigate these issues, foam-generating surfactants have been developed as a method of reducing injected gas phase mobility and increasing oil recovery. This work investigates foam generation and propagation by a proprietary surfactant blend in high-temperature, high-pressure, high-permeability, and high-shear conditions that simulate the environment of a proppant-packed fracture. Bulk foam tests confirmed the aqueous stability and foaming viability of the surfactant at the proposed conditions. Through several series of floods co-injecting methane gas and the surfactant solution through a proppant pack at residual oil saturation, the effects of several injection parameters on apparent foam viscosity were investigated. The foam exhibited an exceptionally high transition foam quality (>95%) and strong shear-thinning behavior. The foam viscosity also linearly decreased with increasing pressure. Another flood series conducted in an oil-free proppant pack showed that swelling of residual oil had no effect on the apparent foam viscosity and was not the reason for the inversely linear pressure dependency. An additional flood series with nitrogen as the injection gas was completed to see if the hydrophobic attraction between the methane and surfactant tail was responsible for the observed pressure trend, but the trend persisted even with nitrogen. In a previous study, the dependence of foam viscosity on pressure was found to be much weaker with a different foaming surfactant under similar conditions. Thus, a better understanding of this important phenomenon requires additional tests with a focus on the effect of pressure on interfacial surfactant adsorption.
Mathematical Modeling of Cell Growth via Inverse Problem and Computational Approach
Authors: Andrusyak Ivanna , Brodyak Oksana , Pukach Petro , Vovk Myroslava
Published: February 2024

A simple cell population growth model is proposed, where cells are assumed to have a physiological structure (e.g., a model describing cancer cell maturation, where cells are structured by maturation stage, size, or mass). The main question is whether we can guarantee, using the death rate as a control mechanism, that the total number of cells or the total cell biomass has prescribed dynamics, which may be applied to modeling the effect of chemotherapeutic agents on malignant cells. Such types of models are usually described by partial differential equations (PDE). The population dynamics are modeled by an inverse problem for PDE in our paper. The main idea is to reduce this model to a simplified integral equation that can be more easily studied by various analytical and numerical methods. Our results were obtained using the characteristics method.