The effectiveness of low‐dose of eCG in timed AI Nelore (Bos indicus) heifers
Authors: Neto A. R. Felisbino , Souza D. F. C. , Campos R. A. , Albertini S. , Bastos M. R. , Freitas B. G. , Colli M. H. A. , Elliff F. M. , Sales J. N. S. , Baruselli P. S. 
Published: March 2024

This study evaluated the efficacy of the administration of different doses of equine chorionic gonadotropin (eCG; 0 IU, 200 IU, or 300 IU) at the time of the progesterone device removal in 2‐year‐old Nelore (Bos indicus) heifers synchronized for fixed‐timed artificial insemination (FTAI). On day 0 (D0), a total of 398 heifers received 2 mg of oestradiol benzoate i.m., 0.53 mg of cloprostenol i.m., and an eight‐day previously used (second use) intravaginal device containing 1 g of progesterone (P4). Eight days later (D8), simultaneous with the P4 device removal, 0.5 mg of oestradiol cypionate i.m. and 0.53 mg of cloprostenol i.m. were administered. At the same time, heifers were randomly assigned to receive one of the following treatments: G‐0 IU (n = 141; no eCG treatment), G‐200 IU (n = 132; treated with 200 IU of eCG), and G‐300 IU (n = 125; treated with 300 IU of eCG). FTAI was performed 48 h after the P4 device removal (D10). Ultrasonographic evaluations were performed at D0, D10, and D17. Heifers were scanned to measure the size of the largest follicle (LF), the presence, number, and size of the corpus luteum (CL), and the ovulation rate. Subsequently, at D40, the heifers underwent scanning to determine the pregnancy rate and identify any twin pregnancies. Additionally, at D70, scans were performed to assess pregnancy loss (PG). Data were analysed by orthogonal contrasts [C1 (eCG effect): control x (200 IU + 300 IU) and C2 (eCG dose effect): 200 IU × 300 IU]. On D0, CL presence was similar between the groups [G‐0 IU = 65.2% (92/141), G‐200 IU = 55.3% (73/132), and G‐300 IU = 63.2% (79/125); p = .16]. No interactions between the presence of CL on D0 and eCG treatment were found for any of the variables (p > .05). The diameter of the LF at FTAI (D10) was not influenced by eCG treatment (p = .22) or eCG dose (p = .18). However, treatment with eCG increased the diameter of the CL at D17 (G‐0 IU = 15.7 ± 0.3 mmb, G‐200 IU = 16.6 ± 0.2 mma, and G‐300 IU = 16.6 ± 0.3 mma; p = .001), regardless of the dose used (p = .94). The ovulation rate was higher in heifers treated with eCG [G‐0 IU = 79.4%b (112/141), G‐200 IU = 90.2%a (119/132), and G‐300 IU = 93.6%a (117/125); p = .002], but there was no effect of eCG dose (p = .36). Pregnancy per AI (P/AI) on D40 [G‐0 IU = 32.6%b (46/141), G‐200 IU = 42.4%a (56/132), and G‐300 IU = 42.4%a (53/125); P = 0.05] and D70 [G‐0 IU = 29.1%b (41/141), G‐200 IU = 40.9%a (54/132), and G‐300 IU = 40.8%a (51/125); p = .02] were higher on heifers that received eCG; however, no dose effect was observed for P/AI on D40 (p = .89) nor D70 (p = .98). Pregnancy loss between D40 and D70 tended to reduce (p = .07) in eCG‐treated heifers without dose effect (p = .91). Heifers with CL at D0 presented a greater follicle diameter (LF) on D10 (With CL = 11.2 ± 0.2 mm and Without CL = 10.2 ± 0.2 mm; p = .05), CL diameter on D17 (With CL = 15.8 ± 0.03 mm and Without CL = 11.8 ± 0.6 mm; p = .01), and ovulation rate [With CL = 95.5% (233/244) and Without CL = 74.7% (115/154); p = .01]. However, no difference in pregnancy rate at D40 (p = .52) and D70 (p = .84) was found. In conclusion, eCG treatment increases ovulation and pregnancy rates of heifers submitted to a FTAI protocol. Furthermore, eCG treatment increases the diameter of the CL after FTAI and reduces pregnancy losses. No dose effect was observed, suggesting Nelore (Bos indicus) heifers respond to 200 IU of eCG treatment for FTAI.