bluuebunny/crossref_metadata_2025 huggingface dataset:
Fundamental Technologies and Recent Advances of Cell-Sheet-Based Tissue Engineering
Authors: Imashiro Chikahiro , Shimizu Tatsuya
Published: January 2021

Tissue engineering has attracted significant attention since the 1980s, and the applications of tissue engineering have been expanding. To produce a cell-dense tissue, cell sheet technology has been studied as a promising strategy. Fundamental techniques involving tissue engineering are mainly introduced in this review. First, the technologies to fabricate a cell sheet were reviewed. Although temperature-responsive polymer-based technique was a trigger to establish and spread cell sheet technology, other methodologies for cell sheet fabrication have also been reported. Second, the methods to improve the function of the cell sheet were investigated. Adding electrical and mechanical stimulation on muscle-type cells, building 3D structures, and co-culturing with other cell species can be possible strategies for imitating the physiological situation under in vitro conditions, resulting in improved functions. Finally, culture methods to promote vasculogenesis in the layered cell sheets were introduced with in vivo, ex vivo, and in vitro bioreactors. We believe the present review that shows and compares the fundamental technologies and recent advances for cell-sheet-based tissue engineering should promote further development of tissue engineering. The development of cell sheet technology should promote many bioengineering applications.
Iron Transport across Symbiotic Membranes of Nitrogen-Fixing Legumes
Authors: Day David A. , Smith Penelope M. C.
Published: January 2021

Iron is an essential nutrient for the legume-rhizobia symbiosis and nitrogen-fixing bacteroids within root nodules of legumes have a very high demand for the metal. Within the infected cells of nodules, the bacteroids are surrounded by a plant membrane to form an organelle-like structure called the symbiosome. In this review, we focus on how iron is transported across the symbiosome membrane and accessed by the bacteroids.
Risk of Contracting COVID-19, Personal Resources and Subjective Well-Being among Healthcare Workers: The Mediating Role of Stress and Meaning-Making
Authors: Krok Dariusz , Zarzycka Beata , Telka Ewa
Published: January 2021

The latest research suggests that the relationships between the risk of contracting COVID-19, personal resources and subjective well-being have rather an indirect character and can include the occurrence of mediating factors related to meaning-making processes and stress experiences. Protection motivation theory offers a theoretical paradigm that enables these associations to be thoroughly investigated and understood. The current study aimed to examine the mediating roles of meaning-making and stress in the relationship of risk of contracting COVID-19 and personal resources (self-efficacy and meaning in life) with subjective well-being among healthcare workers. A total of 225 healthcare workers from hospitals, medical centres and diagnostic units completed a set of questionnaires during the first few months of the COVID-19 lockdown period (March–May 2020). The results revealed that greater self-efficacy and meaning in life were associated with higher cognitive and affective dimensions of subjective well-being, whereas a lesser risk of contracting COVID-19 was only associated with the higher affective dimension. The central finding demonstrated different mediating roles of stress and meaning-making in the relationship of risk of contracting COVID-19 and personal resources with the cognitive and affective dimensions of subjective well-being. This confirmed the applicability of meaning-oriented and stress management processes for understanding how healthcare workers’ well-being is affected during the COVID-19 pandemic.
A Psychosocial Genomics Pilot Study in Oncology for Verifying Clinical, Inflammatory and Psychological Effects of Mind-Body Transformations-Therapy (MBT-T) in Breast Cancer Patients: Preliminary Results
Authors: Cozzolino Mauro , Cocco Stefania , Piezzo Michela , Celia Giovanna , Costantini Susan , Abate Valentina , Capone Francesca , Barberio Daniela , Girelli Laura , Cavicchiolo Elisa , Ascierto Paolo Antonio , Madonna Gabriele , Budillon Alfredo , De Laurentiis Michelino
Published: January 2021

Several studies have highlighted the key role of chronic inflammation in breast cancer development, progression, metastasis, and therapeutic outcome. These processes are mediated through a variety of cytokines and hormones that exert their biological actions either locally or distantly via systemic circulation. Recent findings suggest that positive psychosocial experiences, including psychotherapeutic interventions and therapeutic mind-body protocols, can modulate the inflammatory response by reducing the expression of genes/proteins associated with inflammation and stress-related pathways. Our preliminary results indicate that a specific mind-body therapy (MBT-T) could induce a significant reduction of the release of different cytokines and chemokines, such as SCGFβ, SDF-1α, MCP3, GROα, LIF, and IL-18, in the sera of breast cancer patients compared to a control group, suggesting that MBT-T could represent a promising approach to improve the wellness and outcome of breast cancer patients.
Complementary and Integrative Medicines as Prophylactic Agents for Pediatric Migraine: A Narrative Literature Review
Authors: Yamanaka Gaku , Kanou Kanako , Takamatsu Tomoko , Takeshita Mika , Morichi Shinichiro , Suzuki Shinji , Ishida Yu , Watanabe Yusuke , Go Soken , Oana Shingo , Kawashima Hisashi
Published: January 2021

Complementary and integrative medicines (CIMs) are increasingly used as a preventive antimigraine therapy. In this review, we aimed to summarize the evidence for the efficacy and safety of eight CIMs (riboflavin, coenzyme Q10, magnesium, melatonin, polyunsaturated fatty acids, and combination therapy of feverfew, vitamin D, and ginkgolide B) in pediatric migraine prevention. The level of evidence for riboflavin was relatively high; it was investigated by many studies with five/seven studies demonstrating its efficacy. Five studies investigated the use of melatonin, with one reporting negative results. There was insufficient evidence on the effectiveness of coenzyme Q10, magnesium, and polyunsaturated fatty acids. Combination therapy showed positive potential; however, reports on the individual antimigraine effects of the CIMs were lacking. A definitive conclusion was not reached regarding the specific integrative drugs clinicians should choose for pediatric migraines, owing to low-quality evidence and a limited number of studies. Integrative medications are becoming more common for pediatric migraine prevention as they do not produce serious side effects, and underlying research data suggest their efficacy in preventing migraine. Additional studies are warranted to confirm the role of CIMs in treating patients with migraines.
The Stress of Competing: Cortisol and Amylase Response to Training and Competition
Authors: De Pero Roberta , Minganti Carlo , Cibelli Giuseppe , Cortis Cristina , Piacentini Maria Francesca
Published: January 2021

TeamGym is a popular form of gymnastics, including tumbling (Tu), trampette (Tr) and floor exercises (F) characterized by intensive practice placing high levels of stress on athletes. The aim of the study was to investigate athletes’ stress-related changes during TeamGym training and competition, considering hormonal and enzymatic responses (i.e., salivary cortisol and alpha-amylase). Ten (5 males and 5 females) TeamGym athletes (age: 22–28 y) were tested twice at the same time before training and competition; furthermore, for excluding circadian effect on hormonal and enzymatic responses, they were tested at the same time during a rest day. Alpha-amylase and cortisol were measured 15 min before the beginning of exercise, after each gymnastic equipment performance, and after thirty minutes from the end of the performance. Factorial ANOVA with repeated measures was used to verify differences between training and competition (p < 0.05). Competition elicited higher values of alpha-amylase than training (p ranging from 0.001 to 0.019) and rest (p ranging from 0.001 to 0.019). Cortisol showed no exercise induced increase, and its concentrations were higher prior to training compared to competition. TeamGym responses confirm other sports findings in stating that competition elicits higher stress response than training and suggest that salivary alpha-amylase is a more sensitive marker than cortisol to psychophysiological stress also in gymnastics intermittent performance.
Estimating Production Metric for Ship Assembly Based on Geometric and Production Information of Ship Block Model
Authors: Choi Won-Seok , Kim Dong-Ham , Nam Jong-Ho , Kim Min-Jun , Son Young-Bin
Published: January 2021

To secure technological competitiveness in shipbuilding and offshore industries, the continuous application and development of various technologies is essential. Efficient scheduling in shipyards is an important management task, whereby materials and manpower are allocated at the appropriate time and to the correct workspace. Although some large shipyards ensure effective scheduling and production management through simulations employing advanced technologies, most shipbuilding industries, including small- and medium-sized shipyards, continue to use an index based on past experiences. However, this legacy index, termed the basic unit, involves poor engineering logic; therefore, it does not appropriately reflect a shipyard’s working environment, which changes rapidly in response to technological developments. Although this has led to a demand for improvements in the basic unit, a clear solution has not been presented thus far. In this study, a method for calculating the man-hours required for assembly, which is the basis for preparing the basic unit, is proposed. First, the assembly process is analyzed, and individual activities involved in the assembly process are quantified and formulated into working hours, which is defined as a production metric. Based on a ship’s computerized block model, the geometric properties and production information required for calculating the metric are generated automatically as far as possible; this is to establish a convenient production metric calculation system. The proposed method features complete applicability in new shipyards through a customization. It also serves as a tool for predicting the metric of new ships or comparisons with those of existing ships.
Numerical Modelling of the Effects of the Gulf Stream on the Wave Characteristics
Authors: Ponce de León Sonia , Guedes Soares C.
Published: January 2021

The influence of the Gulf Stream on the wind wave characteristics is investigated. Wave–current interaction inside the current field can result in significant inhomogeneities of the wave field that change the wave spectrum and wave statistics. This study relies on regional realistic simulations using high resolution in time, space and in the spectral space that allow to solve small scale features of the order of 5 km. Wave model simulations are performed with and without ocean currents to understand the impact of the Gulf Stream. Modelled wave spectra are examined along the main axis of the Gulf Stream, and also along a transect that crosses the current. The behavior of significant wave height (Hs), the current speed, as well as the mean wave propagation and the current direction are analyzed at the selected transect locations. It is shown that inside the current the spectral wave energy grows if the wave and the current are aligned and opposed which result in a very peaked and elongated spectrum. The Gulf Stream causes a widening of the spectrum angular distribution. The results indicate that the Hs increases with the current velocity once the waves are inside the Gulf Stream. Most of the time, waves travelled in opposite direction to the current that flows from the SW to the NE, which could explain why inside the Gulf Stream waves are high. The validation of the numerical simulations is performed for Hs using different wave buoy data available in the study region for the winter period of 2019. In addition, one-dimensional wave spectra measured by an NDBC (National Data Buoy Center) wave buoy are compared with the WAM (Wave Advanced Modeling) modelled 1d spectra showing a good correlation. Accounting for ocean currents improves the quality of the simulated results, which is more realistic than only considering waves.
Different Measures of Country Risk: An Application to European Countries
Authors: Bonatti Guido , Ciacci Andrea , Ivaldi Enrico
Published: January 2021

Country Risk (CR) is a relevant instrument to analyze and understand economic performances and relationships between different countries in the actual economic and political international globalized context. The present work develops indexes for the European Union countries by applying three different methods in the field of formative approach. Our aim is to show how robust CR measurements can be developed by operational and easily computable methods. We identify a set of significant variables included in the reference literature. Then, we propose three simple aggregative processes in order to obtain CR measures, at a precise time and over time. As a result, if we compare the outcomes, similar CR rankings emerge. In other words, there are no relevant differences in results also due to different methods of applications. The findings demonstrate that the choice of the aggregation method depends on the willingness of the researcher to baste the analysis with or without weighing and, therefore, on the semantic content that is assigned to the entire research structure. Each analysis should follow a disinterested theoretical–methodological consistency, knowing that the choice of a particular indexing process in the field of aggregation does not significantly alter the nature of the results compared to what would result by applying a different method.
Microstructure and Interfacial Reactions of Resistance Brazed Lap Joints between TC4 Titanium Alloy and 304 Stainless Steel Using Metal Powder Interlayers
Authors: Zhang Pengxian , Fang Zhizhong , Li Shilong
Published: January 2021

In the brazing joint between titanium alloy and stainless steel, a lot of Fe-Ti intermetallic compounds (IMCs) can be easily formed to make joints crack. A lap resistance brazing process with metal powder layers on both sides of the filler metal was used to solve this problem. The microstructure and metallurgical behavior of joints was studied through comparative experiments. The result showed that Nb, V and Cr powders and the solder reacted with the base material to form a new phase, which replaced the Ti-Fe brittle phase in the joint. At the same time, metal powder clusters hindered the diffusion of Ti and Fe elements and improved the distribution of new phases. The established atomic reaction model revealed the metallurgical behavior and formation mechanism of the joints. Therefore, the intervening position of the metal powder layer and the multi-reaction zone structure are the main reasons the shear strength of joints is improved.
Dynamic Performance of Laminated High-Damping and High-Stiffness Composite Structure Composed of Metal Rubber and Silicone Rubber
Authors: Zheng Xiaoyuan , Ren Zhiying , Shen Liangliang , Zhang Bin , Bai Hongbai
Published: January 2021

In this study, a laminated composite damping structure (LCDS) with metal rubber (MR) as matrix and silicone rubber (SR) as reinforcement has been designed. The embedded interlocking structure formed by the multi-material interface of the LCDS can effectively incorporate the high damping characteristics of traditional polymer damping materials and significantly enhance the adjustable stiffness of the damping structure. Based on the periodic cyclic vibration excitation, dynamic tests on different laminated structures were designed, and the damping performance and fatigue characteristics under periodic vibration excitation of the LCDS, based on MR and SR, were explored in depth. The experimental results exhibited that, compared to single-compound damping structures, the LCDS with SR as reinforcement and MR as matrix has excellent stiffness and damping characteristics. The incorporation of the silicon-based reinforcement can significantly improve the performance of the entire structure under cyclic fatigue vibration. In particular, the effects of material preparation and operating parameters on the composite structure are discussed. The effects of MR matrix density, operating frequency, amplitude, and preload on the stiffness and damping properties of the MR- and SR-based LCDS were investigated by the single factor controlled variable method. The results demonstrated that the vibration frequency has little effect on the LCDS damping performance. By increasing the density of the MR matrix or increasing the structural preload, the energy dissipation characteristics and damping properties of the LCDS can be effectively improved. With the increase in vibration excitation amplitude, the energy consumption of the LCDS increases, and the average dynamic stiffness changes at different rates, resulting in the loss factor decreasing first and then increasing. In this study, a damping structure suitable for narrow areas has been designed, which overcomes the temperature intolerance and low stiffness phenomena of traditional polymer rubber materials, and provides effective guidance for the design of damping materials with controllable high damping and stiffness.
Additive Manufacturing Processes in Medical Applications
Authors: Salmi Mika
Published: January 2021

Additive manufacturing (AM, 3D printing) is used in many fields and different industries. In the medical and dental field, every patient is unique and, therefore, AM has significant potential in personalized and customized solutions. This review explores what additive manufacturing processes and materials are utilized in medical and dental applications, especially focusing on processes that are less commonly used. The processes are categorized in ISO/ASTM process classes: powder bed fusion, material extrusion, VAT photopolymerization, material jetting, binder jetting, sheet lamination and directed energy deposition combined with classification of medical applications of AM. Based on the findings, it seems that directed energy deposition is utilized rarely only in implants and sheet lamination rarely for medical models or phantoms. Powder bed fusion, material extrusion and VAT photopolymerization are utilized in all categories. Material jetting is not used for implants and biomanufacturing, and binder jetting is not utilized for tools, instruments and parts for medical devices. The most common materials are thermoplastics, photopolymers and metals such as titanium alloys. If standard terminology of AM would be followed, this would allow a more systematic review of the utilization of different AM processes. Current development in binder jetting would allow more possibilities in the future.
Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach
Authors: Kujawa Joanna , Głodek Marta , Koter Izabela , Ośmiałowski Borys , Knozowska Katarzyna , Al-Gharabli Samer , Dumée Ludovic , Kujawski Wojciech
Published: January 2021

A highly effective method was developed to functionalize ceramic supports (Al2O3 powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity >97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules.
Tryptophan and Kynurenine Enhances the Stemness and Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro and In Vivo
Authors: Pham Hai , Ono Mitsuaki , Hara Emilio , Nguyen Ha , Dang Anh , Do Hang , Komori Taishi , Tosa Ikue , Hazehara-Kunitomo Yuri , Yoshioka Yuya , Oida Yasutaka , Akiyama Kentaro , Kuboki Takuo
Published: January 2021

Aging tissues present a progressive decline in homeostasis and regenerative capacities, which has been associated with degenerative changes in tissue-specific stem cells and stem cell niches. We hypothesized that amino acids could regulate the stem cell phenotype and differentiation ability of human bone marrow-derived mesenchymal stromal cells (hBMSCs). Thus, we performed a screening of 22 standard amino acids and found that D-tryptophan (10 μM) increased the number of cells positive for the early stem cell marker SSEA-4, and the gene expression levels of OCT-4, NANOG, and SOX-2 in hBMSCs. Comparison between D- and L-tryptophan isomers showed that the latter presents a stronger effect in inducing the mRNA levels of Oct-4 and Nanog, and in increasing the osteogenic differentiation of hBMSCs. On the other hand, L-tryptophan suppressed adipogenesis. The migration and colony-forming ability of hBMSCs were also enhanced by L-tryptophan treatment. In vivo experiments delivering L-tryptophan (50 mg/kg/day) by intraperitoneal injections for three weeks confirmed that L-tryptophan significantly increased the percentage of cells positive for SSEA-4, mRNA levels of Nanog and Oct-4, and the migration and colony-forming ability of mouse BMSCs. L-kynurenine, a major metabolite of L-tryptophan, also induced similar effects of L-tryptophan in enhancing stemness and osteogenic differentiation of BMSCs in vitro and in vivo, possibly indicating the involvement of the kynurenine pathway as the downstream signaling of L-tryptophan. Finally, since BMSCs migrate to the wound healing site to promote bone healing, surgical defects of 1 mm in diameter were created in mouse femur to evaluate bone formation after two weeks of L-tryptophan or L-kynurenine injection. Both L-tryptophan and L-kynurenine accelerated bone healing compared to the PBS-injected control group. In summary, L-tryptophan enhanced the stemness and osteoblastic differentiation of BMSCs and may be used as an essential factor to maintain the stem cell properties and accelerate bone healing and/or prevent bone loss.
The Impact of the Lubricant Dose on the Reduction of Wear Dies Used in the Forging Process of the Valve Forging
Authors: Dworzak Łukasz , Hawryluk Marek , Janik Marta
Published: January 2021

The paper presents the results of research on the influence of the settings of lubrication and cooling system parameters (solenoid valve opening time and lubricant feed pressure in terms of its quantity) in order to select the optimal lubricating conditions and thus reduce the wear of the dies used in the first forging operation of the valve forging made of high-nickel steel. Based on the observation of lubrication in the industrial process, it was found that a significant part of the lubricant fails to reach the die cavity, reaching the outside of it, which causes die wear due to seizure resulting from adhesion of the forging material to the tool surface as well as high lubricant consumption and dirt in the press chamber. The authors proposed their own mobile lubricating and cooling system, which allows for a wide range of adjustments and provided with automatic cleaning procedures of the entire system, unlike the fixed lubrication system used so far in the industrial process. First, tests were carried out in laboratory conditions to determine the highest wettability and the lubricant remaining inside the tool cavity. These tests determined the lubrication system parameter settings that ensured that the greatest amount of lubricant remains in the cold die cavity without the forging process. Then, to verify the obtained results, tests were carried out in the industrial process of hot die forging of valve forgings for short production runs of up to 500 forgings. The results were compared with the measurement of changes in the geometry of tools and forgings based on 3D scanning and surface topography analysis with the use of SEM (Scanning Electron Microscope). For the best results (the variant of the setting of the dose and the time of exposure to lubricant), the forging process was carried out with the use of a new tool up to the maximum service life.
Rigorous Mathematical Investigation of a Nonlocal and Nonlinear Second-Order Anisotropic Reaction-Diffusion Model: Applications on Image Segmentation
Authors: Moroşanu Costică , Pavăl Silviu
Published: January 2021

In this paper we are addressing two main topics, as follows. First, a rigorous qualitative study is elaborated for a second-order parabolic problem, equipped with nonlinear anisotropic diffusion and cubic nonlinear reaction, as well as non-homogeneous Cauchy-Neumann boundary conditions. Under certain assumptions on the input data: f(t,x), w(t,x) and v0(x), we prove the well-posedness (the existence, a priori estimates, regularity, uniqueness) of a solution in the Sobolev space Wp1,2(Q), facilitating for the present model to be a more complete description of certain classes of physical phenomena. The second topic refers to the construction of two numerical schemes in order to approximate the solution of a particular mathematical model (local and nonlocal case). To illustrate the effectiveness of the new mathematical model, we present some numerical experiments by applying the model to image segmentation tasks.
Prediction of Maximum Pressure at the Roofs of Rectangular Water Tanks Subjected to Harmonic Base Excitation Using the Multi-Gene Genetic Programming Method
Authors: Bahreini Toussi Iman , Mohammadian Abdolmajid , Kianoush Reza
Published: January 2021

Liquid storage tanks subjected to base excitation can cause large impact forces on the tank roof, which can lead to structural damage as well as economic and environmental losses. The use of artificial intelligence in solving engineering problems is becoming popular in various research fields, and the Genetic Programming (GP) method is receiving more attention in recent years as a regression tool and also as an approach for finding empirical expressions between the data. In this study, an OpenFOAM numerical model that was validated by the authors in a previous study is used to simulate various tank sizes with different liquid heights. The tanks are excited in three different orientations with harmonic sinusoidal loadings. The excitation frequencies are chosen as equal to the tanks’ natural frequencies so that they would be subject to a resonance condition. The maximum pressure in each case is recorded and made dimensionless; then, using Multi-Gene Genetic Programming (MGGP) methods, a relationship between the dimensionless maximum pressure and dimensionless liquid height is acquired. Finally, some error measurements are calculated, and the sensitivity and uncertainty of the proposed equation are analyzed.
Current Knowledge on Microviridin from Cyanobacteria
Authors: do Amaral Samuel Cavalcante , Monteiro Patrick Romano , Neto Joaquim da Silva Pinto , Serra Gustavo Marques , Gonçalves Evonnildo Costa , Xavier Luciana Pereira , Santos Agenor Valadares
Published: January 2021

Cyanobacteria are a rich source of secondary metabolites with a vast biotechnological potential. These compounds have intrigued the scientific community due their uniqueness and diversity, which is guaranteed by a rich enzymatic apparatus. The ribosomally synthesized and post-translationally modified peptides (RiPPs) are among the most promising metabolite groups derived from cyanobacteria. They are interested in numerous biological and ecological processes, many of which are entirely unknown. Microviridins are among the most recognized class of ribosomal peptides formed by cyanobacteria. These oligopeptides are potent inhibitors of protease; thus, they can be used for drug development and the control of mosquitoes. They also play a key ecological role in the defense of cyanobacteria against microcrustaceans. The purpose of this review is to systematically identify the key characteristics of microviridins, including its chemical structure and biosynthesis, as well as its biotechnological and ecological significance.
Evaluation of the Effects of Cuminum cyminum on Cellular Viability, Osteogenic Differentiation and Mineralization of Human Bone Marrow-Derived Stem Cells
Authors: Lee Hyunjin , Song Youngmin , Park Yoon-Hee , Uddin Md. Salah , Park Jun-Beom
Published: January 2021

Background and Objectives: Cuminum cyminum L. has long been used in the treatment of various diseases in multiple geographical regions. This study was performed to determine the effects of C. cyminum methanolic extract (CCT) on the cellular viability, alkaline phosphatase activity and mineralization of human mesenchymal stem cells. Materials and Methods: Bone marrow-derived stem cells were cultured in the presence of CCT at concentrations of 0, 0.001, 0.01, 0.1 and 1 μg/mL. Evaluations of cell morphology were performed on days 1, 3, 7 and 14. Cellular viability was evaluated on days 1, 3, 5 and 7. On the 7th and 14th day, alkaline phosphatase activity measurements and Alizarin red S staining were conducted to assess the osteogenic differentiation of stem cells. A real-time polymerase chain reaction was used to determine the expression levels of RUNX2, BSP, OCN, COL2A1 and β-catenin mRNAs. Results: Stem cells in the control group showed fibroblast-like morphology and the addition of CCT at 0.001, 0.01, 0.1 and 1 μg/mL did not generate noticeable changes in morphology compared with the untreated control group. The application of CCT did not produce significant changes in cellular viability or alkaline phosphatase activity compared with controls. Alizarin Red S staining was significantly increased with the application of CCT. Treatment with CCT increased the expressions of RUNX2, BSP and OCN. Conclusions: These results indicate that CCT enhanced the osteogenic differentiation of stem cells derived from bone marrow by regulating the expressions of RUNX2, BSP and OCN. Thus, the use of CCT may be applied to achieve beneficial effects on the mineralization of stem cells.
Mechanical Power during Veno-Venous Extracorporeal Membrane Oxygenation Initiation: A Pilot-Study
Authors: Belliato Mirko , Epis Francesco , Cremascoli Luca , Ferrari Fiorenza , Quattrone Maria Giovanna , Fisser Christoph , Malfertheiner Maximilian Valentin , Taccone Fabio Silvio , Di Nardo Matteo , Broman Lars Mikael , Lorusso Roberto
Published: January 2021

Mechanical power (MP) represents a useful parameter to describe and quantify the forces applied to the lungs during mechanical ventilation (MV). In this multi-center, prospective, observational study, we analyzed MP variations following MV adjustments after veno-venous extra-corporeal membrane oxygenation (VV ECMO) initiation. We also investigated whether the MV parameters (including MP) in the early phases of VV ECMO run may be related to the intensive care unit (ICU) mortality. Thirty-five patients with severe acute respiratory distress syndrome were prospectively enrolled and analyzed. After VV ECMO initiation, we observed a significant decrease in median MP (32.4 vs. 8.2 J/min, p < 0.001), plateau pressure (27 vs. 21 cmH2O, p = 0.012), driving pressure (11 vs. 8 cmH2O, p = 0.014), respiratory rate (RR, 22 vs. 14 breaths/min, p < 0.001), and tidal volume adjusted to patient ideal body weight (VT/IBW, 5.5 vs. 4.0 mL/kg, p = 0.001) values. During the early phase of ECMO run, RR (17 vs. 13 breaths/min, p = 0.003) was significantly higher, while positive end-expiratory pressure (10 vs. 14 cmH2O, p = 0.048) and VT/IBW (3.0 vs. 4.0 mL/kg, p = 0.028) were lower in ICU non-survivors, when compared to the survivors. The observed decrease in MP after ECMO initiation did not influence ICU outcome. Waiting for large studies assessing the role of these parameters in VV ECMO patients, RR and MP monitoring should not be underrated during ECMO.
Comparison of Nanofiltration with Reverse Osmosis in Reclaiming Tertiary Treated Municipal Wastewater for Irrigation Purposes
Authors: Hafiz MhdAmmar , Hawari Alaa H. , Alfahel Radwan , Hassan Mohammad K. , Altaee Ali
Published: January 2021

This study compares the performance of nanofiltration (NF) and reverse osmosis (RO) for the reclamation of ultrafiltered municipal wastewater for irrigation of food crops. RO and NF technologies were evaluated at different applied pressures; the performance of each technology was evaluated in terms of water flux, recovery rate, specific energy consumption and quality of permeate. It was found that the permeate from the reverse osmosis (RO) process complied with Food and Agriculture Organization (FAO) standards at pressures applied between 10 and 18 bar. At an applied pressure of 20 bar, the permeate quality did not comply with irrigation water standards in terms of chloride, sodium and calcium concentration. It was found that nanofiltration process was not suitable for the reclamation of wastewater as the concentration of chloride, sodium and calcium exceeded the allowable limits at all applied pressures. In the reverse osmosis process, the highest recovery rate was 36%, which was achieved at a pressure of 16 bar. The specific energy consumption at this applied pressure was 0.56 kWh/m3. The lowest specific energy of 0.46 kWh/m3 was achieved at an applied pressure of 12 bar with a water recovery rate of 32.7%.
The Harmonious Interplay of Amino Acid and Monocarboxylate Transporters Induces the Robustness of Cancer Cells
Authors: Yoshida Go J.
Published: January 2021

There is a growing body of evidence that metabolic reprogramming contributes to the acquisition and maintenance of robustness associated with malignancy. The fine regulation of expression levels of amino acid and monocarboxylate transporters enables cancer cells to exhibit the metabolic reprogramming that is responsible for therapeutic resistance. Amino acid transporters characterized by xCT (SLC7A11), ASCT2 (SLC1A5), and LAT1 (SLC7A5) function in the uptake and export of amino acids such as cystine and glutamine, thereby regulating glutathione synthesis, autophagy, and glutaminolysis. CD44 variant, a cancer stem-like cell marker, stabilizes the xCT antiporter at the cellular membrane, and tumor cells positive for xCT and/or ASCT2 are susceptible to sulfasalazine, a system Xc(-) inhibitor. Inhibiting the interaction between LAT1 and CD98 heavy chain prevents activation of the mammalian target of rapamycin (mTOR) complex 1 by glutamine and leucine. mTOR signaling regulated by LAT1 is a sensor of dynamic alterations in the nutrient tumor microenvironment. LAT1 is overexpressed in various malignancies and positively correlated with poor clinical outcome. Metabolic reprogramming of glutamine occurs often in cancer cells and manifests as ASCT2-mediated glutamine addiction. Monocarboxylate transporters (MCTs) mediate metabolic symbiosis, by which lactate in cancer cells under hypoxia is exported through MCT4 and imported by MCT1 in less hypoxic regions, where it is used as an oxidative metabolite. Differential expression patterns of transporters cause functional intratumoral heterogeneity leading to the therapeutic resistance. Therefore, metabolic reprogramming based on these transporters may be a promising therapeutic target. This review highlights the pathological function and therapeutic targets of transporters including xCT, ASCT2, LAT1, and MCT.
SARS-CoV-2 Entry Genes Expression in Relation with Interferon Response in Cystic Fibrosis Patients
Authors: Bitossi Camilla , Frasca Federica , Viscido Agnese , Oliveto Giuseppe , Scordio Mirko , Belloni Laura , Cimino Giuseppe , Pietropaolo Valeria , Gentile Massimo , d’Ettorre Gabriella , Midulla Fabio , Trancassini Maria , Antonelli Guido , Pierangeli Alessandra , Scagnolari Carolina
Published: January 2021

The expression rate of SARS-CoV-2 entry genes, angiotensin-converting enzyme 2 (ACE2), the main viral receptor and the proteases, furin and transmembrane serine protease 2 (TMPRSS2) in cystic fibrosis (CF) individuals is poorly known. Hence, we examined their levels in upper respiratory samples of CF patients (n = 46) and healthy controls (n = 45). Moreover, we sought to understand the interplay of type I interferon (IFN-I) with ACE2, furin and TMPRSS2 by evaluating their gene expression with respect to ISG15, a well-known marker of IFN activation, in upper respiratory samples and after ex vivo IFNβ exposure. Lower ACE2 levels and trends toward the reduction of furin and TMPRSS2 were found in CF patients compared with the healthy controls; decreased ACE2 amounts were also detected in CF individuals with pancreatic insufficiency and in those receiving inhaled antibiotics. Moreover, there was a strong positive correlation between ISG15 and ACE2 levels. However, after ex vivo IFNβ stimulation of nasopharyngeal cells, the truncated isoform (dACE2), recently demonstrated as the IFN stimulated one with respect to the full-length isoform (flACE2), slightly augmented in cells from CF patients whereas in those from healthy donors, dACE2 levels showed variable levels of upregulation. An altered expression of SARS-COV-2 entry genes and a poor responsiveness of dACE2 to IFN-I stimulation might be crucial in the diffusion of SARS-CoV-2 infection in CF.
Rapid Diagnostics of Orthopaedic-Implant-Associated Infections Using Nanopore Shotgun Metagenomic Sequencing on Tissue Biopsies
Authors: Noone J. Christopher , Helmersen Karin , Leegaard Truls Michael , Skråmm Inge , Aamot Hege Vangstein
Published: January 2021

Conventional culture-based diagnostics of orthopaedic-implant-associated infections (OIAIs) are arduous. Hence, the aim of this study was to evaluate a culture-independent, rapid nanopore-based diagnostic protocol with regard to (a) pathogen identification, (b) time to pathogen identification, and (c) identification of antimicrobial resistance (AMR). This prospective proof-of-concept study included soft tissue biopsies from 32 patients with OIAIs undergoing first revision surgery at Akershus University Hospital, Norway. The biopsies were divided into two segments. Nanopore shotgun metagenomic sequencing and pathogen and antimicrobial resistance gene identification using the EPI2ME analysis platform (Oxford Nanopore Technologies) were performed on one segment. Conventional culture-based diagnostics were performed on the other. Microbial identification matched in 23/32 OIAI patients (72%). Sequencing detected additional microbes in 9/32 patients. Pathogens detected by culturing were identified by sequencing within a median of 1 h of sequencing start [range 1–18 h]. Phenotypic AMR was explained by the detection of resistance genes in 11/23 patients (48%). Diagnostics of OIAIs using shotgun metagenomics sequencing are possible within 24 h from biopsy using nanopore technology. Sequencing outperformed culturing with respect to speed and pathogen detection where pathogens were at sufficient concentration, whereas culture-based methods had an advantage at lower pathogen concentrations. Sequencing-based AMR detection may not yet be a suitable replacement for culture-based antibiotic susceptibility testing.
Transamidation of Amides and Amidation of Esters by Selective N–C(O)/O–C(O) Cleavage Mediated by Air- and Moisture-Stable Half-Sandwich Nickel(II)–NHC Complexes
Authors: Buchspies Jonathan , Rahman Md. Mahbubur , Szostak Michal
Published: January 2021

The formation of amide bonds represents one of the most fundamental processes in organic synthesis. Transition-metal-catalyzed activation of acyclic twisted amides has emerged as an increasingly powerful platform in synthesis. Herein, we report the transamidation of N-activated twisted amides by selective N–C(O) cleavage mediated by air- and moisture-stable half-sandwich Ni(II)–NHC (NHC = N-heterocyclic carbenes) complexes. We demonstrate that the readily available cyclopentadienyl complex, [CpNi(IPr)Cl] (IPr = 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene), promotes highly selective transamidation of the N–C(O) bond in twisted N-Boc amides with non-nucleophilic anilines. The reaction provides access to secondary anilides via the non-conventional amide bond-forming pathway. Furthermore, the amidation of activated phenolic and unactivated methyl esters mediated by [CpNi(IPr)Cl] is reported. This study sets the stage for the broad utilization of well-defined, air- and moisture-stable Ni(II)–NHC complexes in catalytic amide bond-forming protocols by unconventional C(acyl)–N and C(acyl)–O bond cleavage reactions.
Recent Discoveries in the Field of Lipid Bio-Based Ingredients for Meat Processing
Authors: Domínguez Rubén , Bohrer Benjamin , Munekata Paulo E. S. , Pateiro Mirian , Lorenzo José M.
Published: January 2021

Current culture and pace of lifestyle, together with consumer demand for ready-to-eat foods, has influenced the food industry, particularly the meat sector. However, due to the important role that diet plays in human health, consumers demand safe and healthy food products. As a consequence, even foods that meet expectations for convenience and organoleptic properties must also meet expectations from a nutritional standpoint. One of the main nutritionally negative aspects of meat products is the content and composition of fat. In this sense, the meat industry has spent decades researching the best strategies for the reformulation of traditional products, without having a negative impact in technological processes or in the sensory acceptance of the final product. However, the enormous variety of meat products as well as industrial and culinary processes means that a single strategy cannot be established, despite the large volume of work carried out in this regard. Therefore, taking all the components of this complex situation into account and utilizing the large amount of scientific information that is available, this review aims to comprehensively analyze recent advances in the use of lipid bio-based materials to reformulate meat products, as well as their nutritional, technological, and sensorial implications.
Evaluation of Ionic Liquids and Ionic Liquids Active Pharmaceutical Ingredients Inhibition in Elastase Enzyme Activity
Authors: Mota Fátima A. R. , Pereira Sarah A. P. , Araujo André R. T. S. , Saraiva M. Lúcia M. F. S.
Published: January 2021

Human neutrophil elastase (HNE) is used as diagnostic biomarker for inflammation/infection. In this work, 10 ionic liquids (ILs) and 11 ionic liquids active pharmaceutical ingredients (ILs-APIs) were tested to evaluate the inhibition effect on the activity of porcine pancreatic elastase enzyme, frequently employed as a model for HNE. The insertion of ionic liquids in some drugs is useful, as the insertion of ILs with inhibitory capacity will also slow down all processes in which this enzyme is involved. Therefore, a spectrophotometric method was performed to the determination of EC50 values of the compounds tested. EC50 values of 124 ± 4 mM to 289 ± 11 mM were obtained, with the most toxic IL for elastase being tetrabutylammonium acetate and the least toxic 1-butyl-3-methylimidazolium acetate. Moreover, sodium salicylate (raw material) presented the lower and benzethonium bistriflimide the higher EC50 when compared with all the IL-APIs tested. This work provides significant information about the effect of the studied IL and IL-APIs in elastase enzyme activity.
Inhibitory Effect of Nepeta deflersiana on Climax Bacterial Community Isolated from the Oral Plaque of Patients with Periodontal Disease
Authors: Ahmad Irfan , Irfan Safia , Abohashrh Mohammed , Wahab Shadma , Abullais Shahabe Saquib , Javali Mukhatar Ahmed , Nisar Nazima , Alam Mohammad Mahtab , Srivastava Saurabh , Saleem Mohd , Zaman Gaffar Sarwar , Ahmad Irshad , Mansuri Nasrin
Published: January 2021

Background: The red-complex bacteria are one of the most significant complexes found simultaneously in subgingival plaque next to the periodontal pocket. The current antibacterial treatment is not adequate, and multidrug resistance to it is developing. Henceforth, the antibacterial effect of the ethanolic extract of Nepeta deflersiana was put to test against red-complex bacteria in patients with chronic periodontitis. Methods: Well diffusion and micro broth dilution procedure by Alamar blue were applied to assess the zone of inhibition (ZOI), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Anti-virulence efficacies of the plant extract that comprise of adherence and formation of biofilms were examined by the process of adherence and biofilm production assay. Results: The crude extract of Nepeta deflersiana exhibited significant inhibitory outcome against periodontopathic bacteria with noteworthy MIC (0.78–3.12 mg/mL), inhibitory zone (12–20 mm), as well as MBC (3.12–12.50 mg/mL). The N. deflersiana extract inhibited bacterial adhesion ranging from 41% to 52%, 53% to 66%, and 60% to 79% at the given MIC × 0.5, MIC × 1, and MIC × 2 in succession. Substantial suppression was also developed in the biofilm production of the investigated periodontopathic strains following exposure to numerous concentrations of N. deflersianan extract for a period of 24 and 48 h. Conclusion: These outcomes divulge a new concept that N. deflersiana extract can be utilized to manufacture valuable antibacterial compounds to treat chronic and acute periodontitis. This identifies N. deflersiana as an essential natural source for future drug development.
Lactoferrin: A Glycoprotein Involved in Immunomodulation, Anticancer, and Antimicrobial Processes
Authors: Rascón-Cruz Quintín , Espinoza-Sánchez Edward A. , Siqueiros-Cendón Tania S. , Nakamura-Bencomo Sayuri I. , Arévalo-Gallegos Sigifredo , Iglesias-Figueroa Blanca F.
Published: January 2021

Lactoferrin is an iron binding glycoprotein with multiple roles in the body. Its participation in apoptotic processes in cancer cells, its ability to modulate various reactions of the immune system, and its activity against a broad spectrum of pathogenic microorganisms, including respiratory viruses, have made it a protein of broad interest in pharmaceutical and food research and industry. In this review, we have focused on describing the most important functions of lactoferrin and the possible mechanisms of action that lead to its function.
Synthesis and Investigation of Tetrahydro-β-carboline Derivatives as Inhibitors of Plant Pathogenic Fungi
Authors: Buaban Koonchira , Phutdhawong Weerachai , Taechowisan Thongchai , Phutdhawong Waya S.
Published: January 2021

A series of tetrahydro-ß-carbolines substituted with an alkyl or acyl side chain was synthesized and screened for its antifungal activity against plant pathogenic fungi (Bipolaris oryzae, Curvularia lunata, Fusarium semitectum, and Fusarium fujikuroi). The structure activity relationship revealed that the substituent at the piperidine nitrogen plays an important role for increasing antifungal activities. In this series, 2-octyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole (3g) displayed potent antifungal activities with a minimum inhibitory concentration of 0.1 μg/mL, including good inhibitory activity to the radial growth of fungus at a concentration of 100 μg/mL compared to amphotericin B.