bluuebunny/crossref_metadata_2025 huggingface dataset:
DEVELOPMENT OF AN AUTOMATED BIBLIOGRAPHIC SYSTEM
Authors: Tsybulnyk Serhii , Bidnyk Danylo , Pivtorak Diana
Published: July 2022

Most research and higher education institutions of Ukraine are funded by the state. Almost all research is conducted mainly in the public sector, while private universities and companies usually do not conduct any research. Public higher education institutions are financed from the state budget and lack of funds is one of the main factors hindering the introduction of new information technologies and systems into the daily routine. Analysis of scientific achievements using bibliographic information has become a significant part of scientific and technical management in many countries around the world, but unlike Slovenia and Poland, Ukraine does not have a single bibliographic database and relevant search interfaces. Therefore, the aim of the work is to develop an automated bibliographic system that will simplify the process of creating, editing, searching, sorting, statistics, deleting bibliographic information of research and teaching staff and will automate the formation of personalized reports. To implement the proposed automated system, a review of existing databases of bibliographic information and search interfaces was conducted. It is concluded that they cannot provide Ukrainian scientists with the necessary functionality to form bibliographic descriptions of their own research papers. To design the architecture of a mobile application based on the Android operating system, a multi-level architecture class and an MVVM template were selected. Designed architecture contains three levels and five layers on which separate subsystems are located. The Pixel emulator and Xiaomi Redmi 5 Plus smartphone were used to test the application on different screen sizes, as well as on different versions of the software interface. The application was developed in the AndroidStudio software environment. The design of the entered data according to two standards was implemented, namely: DSTU 8302-2015 and IEEE. Algorithms for searching and sorting records according to the necessary criteria are implemented. Saving the list of all necessary bibliographic descriptions in text format is implemented. Additionally, the possibility of sending it to e-mail is implemented. In the future it is planned to expand the list of standards for bibliographic information and improve search and sorting algorithms.
The Influence of Surgeon Sex on Adverse Events Following Primary Total Hip Arthroplasty
Authors: Jolbäck Per , Rogmark Cecilia , Bedeschi Rego De Mattos Camila , Chen Antonia F. , Nauclér Emma , Tsikandylakis Georgios
Published: July 2022

Background:

Stereotypes may prevail, but little is known about the influence that the sex of an orthopaedic surgeon may have on outcomes. In a recently published study, there were no differences in the rate of adverse events following total hip arthroplasties (THAs) performed by female or male orthopaedic surgeons. The objective of the present study was to investigate whether there was any difference in adverse events within 90 days following a primary THA performed by either a male or female surgeon in Sweden.

Methods:

A retrospective study was performed to evaluate primary THAs performed for osteoarthritis between 2008 and 2016 at 10 hospitals in western Sweden. Local hospital data were linked with the Swedish Hip Arthroplasty Register (SHAR) and with a regional patient register. Data collected from local hospitals and the SHAR included surgeon-related information (e.g., sex, annual volume, and level of training) and patient-related information (e.g., age, sex, and Elixhauser comorbidity index). Adverse events were retrieved from the regional patient register. The definition of adverse events followed the SHAR definition of adverse events. Mixed models were used to investigate the impact of surgeon sex on adverse events.

Results:

A total of 11,993 primary THAs were performed by 200 surgeons, of whom 17.5% were women. The proportions of adverse events within 90 days were similar for female (6%) and male (7%) surgeons. No association was found between surgeon sex and adverse events (adjusted odds ratio, 0.72; 95% confidence interval, 0.52 to 1.00) when all surgeons (both attendings and residents) were included in the analysis. A sensitivity analysis that included attendings only yielded similar results (adjusted odds ratio, 0.88; 95% confidence interval, 0.60 to 1.29).

Conclusions:

Despite a small tendency toward lower rates of adverse events at 90 days after THAs performed by female surgeons, there was no significant association between surgeon sex and the risk of adverse events following THA.

Level of Evidence:

Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Effects of COVID-19 on Geographical Trends in the Orthopaedic Surgery Residency Match
Authors: Holderread Brendan M. , Han Alex , Mand Davinder S. , Liu Jonathan , Harris Joshua D. , Liberman Shari R.
Published: July 2022

BACKGROUND:

Historically, medical students often match within the same geographic location or to an orthopaedic surgery residency program affiliated with their medical school. The objective of this investigation was to determine differences in geographic trends between orthopaedic residents matching before and during the Coronavirus-19 (COVID-19) pandemic.

METHODS:

This study analyzed 2 groups of orthopaedic residents: Pre-COVID cohort (years 2016-2020) and COVID-impacted cohort (year 2021). A list of accredited orthopaedic surgery residency programs (n = 202) was obtained. Orthopaedic residency program webpages were located (region [n = 4], division [n = 9], state [n = 50]). For each resident, their medical school and year of postgraduate training were recorded. Year 2021 resident information was obtained from the orthopaedic residency program webpages, social media accounts, and medical school match lists. Residency programs affiliated with a medical school were also assigned. Descriptive statistics were performed. Two sample Student t tests with Bonferroni correction applied to p-values (α < 0.05 significant) were performed.

RESULTS:

There were 4,832 residents analyzed (4,074 in Pre-COVID cohort; 758 in COVID-impacted cohort [758/868 of all positions in 2021 Match]). Statistically significant differences were detected between the COVID-impacted cohort (39.6%, p < 0.001) matching in the same state as their medical school (Pre-COVID 33.1%) and the COVID-impacted cohort (28.0%, p < 0.001) matching to a residency program affiliated with their medical school (Pre-COVID 21.2%). In the COVID-impacted cohort, students who matched in state matched to their home program more frequently (69.3%) compared with the Pre-COVID cohort (60.5%). Geographically, there was a difference in the COVID-impacted cohort (52.5%, p < 0.011) matching in the same division (Pre-COVID 47.5%). No statistically significant differences were identified for residents matching to the same region as their medical school (Pre-COVID 60.1%; COVID-impacted 61%, p = 0.968).

CONCLUSION:

Residents matching in the same state, in the same division, and to a residency program affiliated with their medical school increased significantly in the COVID-impacted cohort. There was no difference between cohorts matching in the same region as their medical school.

Level of Evidence:

Observational/Cross-Sectional
Transferências Voluntárias e o Efeito Flypaper
Authors: Oliveira da Luz Estela , Nossa Silvania Neris , Soares Monte-mor Danilo , Bezerra Filho João Eudes
Published: July 2022

Objetivo da pesquisa: Examinar a ocorrência do efeito flypaper nos repasses de transferências voluntárias da União para os municípios brasileiros. Foram analisados os fatores complexidade e visibilidade explorados pelos autores Tuckman e Chang (1991), Rios e Costa (2005), Araújo e Siqueira (2015), acrescido do fator coligações políticas, no cenário dos dispositivos da Emenda Constitucional (EC) 86/2015 e EC 95/2019.
Originalidade: Apesar de existir vários estudos sobre orçamento impositivo no Brasil (Santos & Gasparine, 2020; Godoy, 2014; Gontijo, 2017), o diferencial desta pesquisa em relação às demais se dá pela análise da ocorrência do efeito flypaper dos repasses decorrentes das emendas orçamentária impositivas (Lei Orçamentária da União).
Metodologia: Foi utilizado o método de análise multivariada para dados em painel sobre dados secundários coletados de 5.484 municípios brasileiros para testar o efeito flypaper nas contas totais das despesas correntes e de capital, em relação às transferências voluntárias recebidas.
Resultados: Foi possível evidenciar o efeito flypaper para as despesas orçamentárias de capital, que têm aumentado acima da capacidade da economia dos municípios em proverem recursos para manutenção e custeios dos bens instalados. De forma associada, questões partidárias e políticas tendem a direcionar recursos para suas bases, sem realizarem estudos técnicos que viabilizam manutenções futuras dos investimentos decorrentes das emendas parlamentares. Os resultados também sugerem que mudanças na complexidade tributária podem favorecer o investimento em bens estruturais.
Contribuições teóricas e práticas: O estudo do flypaper, na administração pública, ratifica as análises do ministério do Planejamento junto ao Governo Federal brasileiro, no que tange à efetividade de aplicação dos recursos públicos; seu efeito pode trazer repercussões na geração de aumento dos gastos públicos; favorece a imperícia do recolhimento de tributos e constantes déficits nos cofres municipais; promove a nulidade de retorno financeiro dos recursos públicos, talvez na forma de redução tributária.
Princípios da Responsabilidade Social e Cooperação Internacional para o Desenvolvimento: Uma Análise do Programa Lagoas do Norte: Uma Análise do Programa Lagoas do Norte
Authors: De Oliveira Sousa Francílio , Neto Alexandre Rabêlo , Gomes Campelo Filho Eulálio , De Menezes  Urra Monique
Published: July 2022

Objetivo da Pesquisa: Analisar o benefício dos princípios da responsabilidade social das Normas Brasileiras NBR-ISO 26000 em um programa de cooperação internacional para o desenvolvimento.
Enquadramento teórico: O artigo apresenta uma evolução do conceito de Responsabilidade social, mediante as teorias relacionadas à cooperação internacional para o desenvolvimento – CID, transparência e accountability.
Metodologia: O estudo utilizou uma abordagem qualitativa, de caráter exploratória, ancorada na triangulação de dados documentais e análise de conteúdo de entrevistas semiestruturadas com os gestores do Programa Lagoas do Norte e cidadãos que residem na área de abrangência do projeto em Teresina.
Resultados: Os princípios da responsabilidade social da NBR-ISO 26000 impactaram a gestão pública do município de Teresina em sua capacidade de desenvolvimento e gerenciamento de intervenções urbanas e desenvolvimento econômico e social.
Originalidade: Os resultados contribuem para suprir a ausência, na literatura brasileira, de artigos que façam uma discussão teórica acerca dos aspectos relacionados à cooperação internacional para o desenvolvimento – CID, transparência e accountability.
Contribuições teóricas e práticas: Uma vez que a ISO 26000 aponta para a necessidade de uma investigação mais criteriosa em relação às partes interessadas, esse estudo contribui em uma perspectiva prática para a gestão dos projetos de cooperação internacional de desenvolvimento – CID, no que diz respeito à relação com empresas, governos, mídia, fornecedores, organizações pares e a comunidade em que opera.
Hydrochemical Characteristics and Quality Assessment of Groundwater under the Impact of Seawater Intrusion and Anthropogenic Activity in the Coastal Areas of Zhejiang and Fujian Provinces, China
Authors: Fu Tengfei , Qi Chen , Wang Zhenyan , Li Chenzhe , Liu Wenquan , Fu Yushan , Chen Guangquan , Su Qiao , Xu Xingyong , Yu Hongjun
Published: July 2022

Coastal groundwater is an important resource in the developed region associated with human health and sustainable economic development. To identify the origins of salinity and evaluate the impact of water-rock interactions, seawater intrusion (SWI), and evaporation on groundwater in the coastal areas of Zhejiang and Fujian provinces, a comprehensive investigation was performed. Meanwhile, nitrate and fluoride indicators resulting from the anthropogenic activity and SWI were also considered. At last, the water quality index (WQI) of coastal groundwater was evaluated with geochemical and multivariate statistical methods. The results indicated that (1) the groundwater in coastal areas of Zhejiang and Fujian provinces has been affected by SWI to varying degrees. The analysis of selected ion ratios (Na+/Cl− and Br−/Cl−) and isotopic compositions showed that SWI is the predominant cause of increasing salinity in the groundwater of Zhejiang Province, while the cause is water-rock interactions (ion exchange and mineral weathering) in Fujian Province. The hydrochemical evolution path of groundwater in Zhejiang Province is Ca/Mg-HCO3 to Na-Cl, while a different pattern of Ca/Mg-HCO3 to Na (Mg/Ca)-Cl occurs in Fujian Province. However, the trend of SWI development in both provinces was freshening. (2) Nitrification, sewage infiltration, and SWI increased the NO3− content in groundwater. Some of the NO3− concentration in Fujian Province exceeds the standard, and the nitrogen pollution was more serious than in Zhejiang Province. The F− content in coastal groundwater was affected by SWI and mineral dissolution; the F− content in Zhejiang Province was higher than in Fujian Province, which was close to the groundwater standard limit. The average WQI value of Zhejiang was 103.61, and the WQI of Fujian was 61.69, indicating that the coastal groundwater quality in Fujian Province was better than in Zhejiang Province. The results of the study revealed the impact of SWI and anthropogenic activity on groundwater in the southern coastal zone of China and will be valuable for sustainable groundwater resource management.
A Novel Proposed CNN-SVM Architecture for ECG Scalograms Classification
Authors: Ozaltin Oznur , Ozgur Yeniay
Published: July 2022

Nowadays, the number of sudden deaths due to heart disease is increasing with the Coronavirus pandemic. Thus, Electrocardiogram (ECG) signals automatic classification is of vital importance for diagnosis and treatment. Thanks to deep learning algorithms, the classification can be performed without manual feature extraction. In this study, we propose a novel convolutional neural networks (CNN) architecture. Further, the proposed CNN can be extracted automatic features from images. Here, we classify a real ECC data set using our proposed CNN that includes 34 layers. While this dataset is one-dimensional signals, these are transformed into two-dimensional scalograms by continuous wavelet transform (CWT). In addition, we compare it with well-known architectures: AlexNet and SqueezeNet. When we classify ECG scalograms with proposed CNN, we find it more effectively than others. Although the results are very good, we benefit from support vector machines (SVM). Essentially, our main aim is to achieve the best classification results on account of health. Thus, we modify the proposed CNN with SVM. As a result, we achieve the highest success with an accuracy of 99.21% from our proposed CNN-SVM.
Seasonal rainfall pattern using coupled neural network-wavelet technique of of southern Uttarakhand, India
Authors: Singh Shekhar , Vishwakarma Dinesh Kumar , Kumar Rohitashw , Kushwaha N. L.
Published: July 2022

Uttarakhand, the land of peaceful, sanctity and venerable beauty. Most of its geographical area comes under the hill and the remaining areas lives in the belt of Tarai plains. The patchiness in topography has led to a significant change in the rainfall pattern. It is playing a vital role in water resource management and the field of hydrology. With the help of a statistical and hybrid model of Wavelet-ANN (WANN), the seasonal rainfall variation for theperiod of 1901 to 2016 in the southern part of Uttarakhand namely, Almora, Kashipur, Landsdown and Mukteswar was studied.The study was done on a seasonalbasis ofmonthly rainfall data.The statistical results suggested that the mean maximum rainfall was found to be 746.82 mm, 1586.58 mm, 1060.53 mm and 964.43 mm for Almora, Lansdown, Kashipur and Mukteswar respectively. For each season, four WANN modelshave been developed (total of sixteen models) by varying the number of hidden neurons. The results suggested that only one WANN model was not sufficient to predict the rainfall of all stations. Every station has a specific networked model which could model the data more preciously. The findings illustrated that the hybrid model of WANNhavingNetwork (4-7-1) was found best model for the Landsdown stations among all the stations.
Imaging investigation of cervicocranial artery dissection by using high resolution magnetic resonance VWI and MRA : qualitative and quantitative analysis at different stages
Authors: Ma Weiqiong , Zhou kexin , Lan Bowen , Li Wuming , Jiang Guihua
Published: July 2022

Background To explore the value of magnetic resonance angiography(MRA) and high resolution magnetic resonance vessel wall imaging(HRMR-VWI) in cervicocranial artery dissection(CCAD) diagnosis and disease staging.On the basis of qualitative evaluation,this study also extract the dynamic changes in vessel wall in different vessel segments to identify imaging indicators for the quantitative evaluation of CCAD. Methods We retrospectively enrolled 33 patients with CCAD（37 branches）with conventional MRA and HRMR-VWI examinations.Two radiologists independently analyzed imaging features of vessel wall and lumen in the different stages,and the typical sign detection of artery dissection were compared between MRA and HRMR-VWI.Then the parameters of vessel wall was quantitatively evaluated by Vesselmass.Results HRMR-VWI revealed typical sign detection of artery dissection in all patients in the acute and subacute stage.Among them,the intimal flap/double lumen sign ditection were more common than the MRA,there was significant difference(P=0.01).MRA revealed typical sign detection of artery dissection in more than half the patients,and the detection was no significant difference at the chronic stage between MRA and HRMR-VWI(P=1.000/1.000/0.761).In the acute and subacute stage, the typical sign detection of artery dissection revealed by HR-MRI was higher than the observations in the chronic stage(P=0.028),while there was no significant difference by MRA(P=0.682/1.000). The relative signal intensity of IMH and wall enhancement, wall thickness index were gradually decreased from the acute stage to the chronic stage;Significant differences were found between the recovered group of chronic stage and the acute stage(P＜0.05),but it is no significant difference between the recovered group of chronic stage and the acute stage(p=1.000、0.061).Conclusions As the only noninvasive imaging technology, HRMR-VWI displays the structure of the vessel wall in vivo, showing not only excellent performance in the early diagnosis of CCAD, but also describing the dynamic changes in the qualitative and quantitative characteristics of vessel wall. It also helps to guide the disease course, staging, prognosis, and treatment of CCAD.Although the diagnostic efficacy of MRA was not as good as HRMR-VWI, it should be the first choice of method for routine examination in evaluating CCAD, especially at the chronic stage of CCAD.
Spectral CT - a new supplementary method for preoperative assessment of pathological grades of esophageal squamous cell carcinoma
Authors: Wang Yi , Tian Weizhong , Tian Shuangfeng , He Liang , Xia Jianguo , Zhang Ji
Published: July 2022

Background
Spectral CT imaging parameters have been reported to be useful in the differentiation of pathological grades in different malignancies. This study aims to investigate the value of spectral CT in the quantitative assessment of esophageal squamous cell carcinoma (ESCC) with different degrees of differentiation༎
Methods
There were 191 patients with proven ESCC who underwent enhanced spectral CT from June 2018 to March 2020 retrospectively enrolled. These patients were divided into three groups based on pathological results: well differentiated ESCC, moderately differentiated ESCC, and poorly differentiated ESCC. Virtual monoenergetic 40keV-equivalent image (VMI40keV), iodine concentration (IC), water concentration (WC), effective atomic number (Eff-Z), and the slope of the spectral curve(λHU) of the arterial phase (AP) and venous phase (VP) were measured or calculated. The quantitative parameters of the three groups were compared by using one-way ANOVA and pairwise comparisons were performed with LSD. Receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic performance of these parameters in poorly differentiated groups and non-poorly differentiated groups.
Results
There were significant differences in VMI40keV, IC, Eff-Z, and λHU in AP and VP among the three groups (all p < 0.05) except for WC (p > 0.05). The VMI40keV, IC, Eff-Z, and λHU in the poorly differentiated group were significantly higher than those in the other groups both in AP and VP (all p < 0.05). In the ROC analysis, IC performed the best in the identification of the poorly differentiated group and non-poorly differentiated group in VP (AUC = 0.729, Sensitivity = 0.829, and Specificity = 0.569 under the threshold of 21.08 mg/ml).
Conclusions
Quantitative parameters of spectral CT could offer supplemental information for the preoperative differential diagnosis of ESCC with different degrees of differentiation.
Bilateral orofacial clefts confer increased risk of psychiatric morbidity relative to unilateral clefts
Authors: Tillman Karin , Hakelius Malin , Höijer Jonas , Ramklint Mia , Ekselius Lisa , Nowinski Daniel , Papadopoulos Fotios
Published: July 2022

Background
Nonsyndromic orofacial clefts (OFC) are associated with neurodevelopmental disorders. The aim of this study was to compare children with bilateral clefts with unilateral clefts regarding psychiatric disorders.
Methods
We utilized data on all children born in Sweden between 1973 and 2012 with unilateral cleft lip (UCL), bilateral cleft lip (BCL), unilateral cleft lip and palate (UCLP) and bilateral cleft lip and palate (BCLP). We performed Cox regression analyses with direct comparisons between bilateral and unilateral clefts compared to matched community cohorts and adjusted for confounders.
Results
Children with BCL showed risk increases for any psychiatric disorder (aHR 2.13, 95% CI 1.04–4.36), including intellectual disability (aHR 5.31, 95% CI 1.29–21.78) compared to UCL. Children with BCLP demonstrated risk increases for any psychiatric disorder (aHR 1.55, 95% CI 1.20–2.01), including speech and language disorders (aHR 1.99, 95% CI 1.00-3.97), neurodevelopmental disorders (aHR 1.66, 95% CI 1.11–2.47) and other psychiatric disorders (aHR 1.54, 95% CI 1.11–2.15), such as personality disorders (aHR 5.76, 95% CI 2.13–15.55) compared to UCLP.
Conclusions
This large nationwide register study shows for the first time that individuals with bilateral clefts demonstrate elevated risks of psychiatric disorders compared to unilateral clefts.
Association between Body Mass Index Structure and Physical Activity, Sleep, Sedentary Behavior, Health, Stress, Emotional Intelligence, and Healthy Eating: A Study of 8,759 People in Lithuania
Authors: Skurvydas Albertas , Lisinskiene Ausra , Majauskienė Daiva , Valanciene Dovile , Dadeliene Ruta , Fatkulina Natalja , Mockeviciene Daiva , Sarkauskiene Asta
Published: July 2022

Objective: To determine how the body mass index (BMI) structure (underweight, normal weight, overweight, and obesity grades I and II) affects the physical activity structure (light physical activity, moderate physical activity, and vigorous physical activity), sleep, sedentary behaviour, eating habits, stress, depression, health, and emotional intelligence.Results: We determined that health and stress depended on the BMI structure only among women and Emotional Intelligence depended on the body mass index structure only among men. Emotional Intelligence was higher among overweight and obese men than normal weight and underweight men. We found that light physical activity in women and men, and especially vigorous physical activity METs structures, sedentary, significantly were associated with the BMI structure, whereas sleep METs didn’t depend on the BMI structure in women and men. As BMI increases, vigorous physical activity significantly decreases in men and women. We identified a significant direct correlation relationship between vigorous physical activity and eating breakfast. We found that of all energy expenditure determinants vigorous physical activity had the best correlation with the BMI structure among women and men. We identified that BMI was closely and directly associated with the frequencies of overeating and skipping breakfast among women and men.
Perceptions of Social Media Safety in Japan
Authors: Kudo Noriko , Manabe Masae , Liew Kongmeng , Wakamiya Shoko , Ayaya Satsuki , Kumagaya Shinichiro , Aramaki Eiji
Published: July 2022

Background and objective: We often treat the dangers of social networking as similar across sites. However, this study demonstrates that users' perceptions of social media safety differs between social media platforms.Methods: We conducted a questionnaire of Japanese social media users (N = 7198) using an adapted version of the Psychological Safety Scale, to investigate the relationship between perceived psychological safety and the operational statistics associated with each social media platform. We then conducted data collection examining subjective classification from a different set of users (N = 2304).Results: Of the social networking sites examined, “Yahoo! Chiebukuro" and "note" were rated with high perceived psychological safety.The higher the number of monthly active users in Japan, the higher the psychological safety score, and there was also a positive correlation between login frequency and psychological safety. In addition, high openness and high self-disclosure were associated with low psychological safety.Conclusions: The psychological safety of social media sites was related to the number of domestic users, the number of logins, openness, and self-disclosure. These results of this study can be used as a reference for the design of social media platforms.
Impact of the application of NRS2002 by nurses and dieticians in a tertiary care centre: reliability and clinical outcomes
Authors: Deschepper Mieke , Duprez Veerle , Lapauw Bruno , Eeckloo Kristof , Marchand Sophie
Published: July 2022

Objective: To evaluate the level of agreement between nurse and dietician nutritional risk assessments, using the Nutritional Risk Screening 2002 (NRS2002) based risk screening protocol, to explore the effect of falsely labeling patients `not at risk` for malnutrition, and the effect of delayed involvement of the dietician (STD) on the length of stay (LoS).Methods: Including all patients hospitalized in a tertiary care center between January 2017 and December 2019 screened for malnutrition by both a nurse and a dietician. The inter-rater reliability is evaluated using Cohen’s Kappa. The relation between STD and the patient classification (PCET) is assessed by a linear mixed effect model. The relation between the LoS and PCET is evaluated with the Kaplan-Meier method and multivariate Cox regression including STD with pathology group and severity of illness as random effect.Results: 9085 patients are assessed by nurse and dietician. 72% of all assessments agree (Kappa=0.44 [0.43-0.46]). The dietician is involved later for patients falsely labeled ‘not at risk’ (1.06 [0.92 – 1.20] days; p<0.001). Compared to patients where the dietician is involved within three days, the LoS is 7.37 days (Hazard Ratio (HR): 0.51 [0.43-0.61]) longer for patients falsely labeled ‘not at risk’, while only 3.51 days (HR: 0.72 [0.64-0.80]) longer for patients correctly labeled ‘at risk’ . Conclusions: Agreement of screening for malnutrition between nurses and dieticians is weak. Avoiding falsely labeling patients `not at risk` should be a main concern upon patient admission as later involvement of dieticians is correlated with a longer LoS.
Mitochondrial DNA is a frequent target of HBV integration
Authors: Giosa Domenico , Lombardo Daniele , Musolino Cristina , Chines Valeria , Raffa Giuseppina , Tocco Francesca Casuscelli di , D’Aliberti Deborah , Caminiti Giuseppe , Saitta Carlo , Alibrandi Angela , Cigliano Riccardo Aiese , Romeo Orazio , Navarra Giuseppe , Raimondo Giovanni , Pollicino Teresa
Published: July 2022

Hepatitis B virus (HBV) may integrate into the genome of an infected cell and contribute to hepatocarcinogenesis. In this study, we applied a new high-throughput HBV integration sequencing approach that allows sensitive identification of HBV integration sites and enumeration of integration clones. We identified 3,339 HBV integration sites in paired tumour and non-tumour tissue samples from 7 patients with hepatocellular carcinoma (HCC). A total of 2,107 clonally expanded integrations were detected: 1,817 in tumour and 290 in non-tumour tissues. Moreover, we detected significant enrichment of clonal integrations in mitochondrial DNA (mtDNA), and HBV integration in mtDNA preferentially involved OXPHOS genes in tumours and the regulatory D-loop region in non-tumour tissues. We also found that HBV RNA sequences may be imported into the mitochondria of hepatoma cells, polynucleotide phosphorylase (PNPASE) is involved in this import, and HBV RNA might be involved in the process of HBV integration into mtDNA. Our data suggest a novel mechanism by which HBV insertion might contribute to HCC development.
Screening of single nucleotide polymorphisms within HLA region related to hematopoietic stem cell transplantation using MassARRAY technology
Authors: Tsai Shu-Hui , Chang Pi-Yueh , Wen Ying-Hao , Lin Wei-Tzu , Hsu Fang-Ping , Chen Ding-Ping
Published: July 2022

A growing number of studies showed that single nucleotide polymorphisms (SNPs) in the human leukocyte antigen (HLA)-related genes were associated with the outcome of hematopoietic stem cell transplantation (HSCT). Thus, other SNPs located nearby the classical HLA genes must be considered in HSCT. We evaluated the clinical feasibility of MassARRAY by comparing to Sanger sequencing. The PCR amplicons with each one of the 17 loci that were related to the outcomes of HSCT published by our previous study were transferred onto a SpectroCHIP Array for genotyping by mass spectrometry. The sensitivity of MassARRAY was 100% (1,419/1,419) and the specificity was 98.9% (470/475), where the positive predictive value (PPV) was 99.6% (1,419/1,424) and the negative predictive value (NPV) was 98.9%. MassARRAY is high-throughput, which can accurately analyze multiple SNPs at the same time. Based on these properties, it could accelerate the time of matching the genotype between the graft and the recipient before transplantation.
Modulation of the microbiota across different intestinal segments by Rifaximin in PI-IBS mice
Authors: Zhang Shengyan , Hong Gaichao , Li Gangping , Qian Wei , Jin Yu , Hou Xiaohua
Published: July 2022

Background
Rifaximin has been increasingly applied in irritable bowel syndrome (IBS) treatment. Whether there were differences in the effects of rifaximin on microbiota from different intestinal segments, especially the small intestine where rifaximin predominantly acted, has not been confirmed.
Methods
In this study, we used Trichinella spiralis infection to induce post infectious irritable bowel syndrome (PI-IBS) and measured visceral sensitivity of mice by means of abdominal withdrawal reflex (AWR) tests to colorectal distention (CRD). We compared the effects of rifaximin on the composition of ileal, colonic mucosal and fecal microbiota in PI-IBS mice.
Results
Rifaximin significantly reduced AWR scores and increased pain threshold in PI-IBS mice, and this effect was associated with the change in the relative abundance of ileal mucosal microbiota. Rifaximin could obviously decrease ileum mucosal microbiota alpha diversity assessed by Shannon microbial diversity index. Meanwhile, the analysis of beta diversity and relative abundance of microbiota at phylum, family and genus levels showed that rifaximin could improve the microbiota structure of ileal mucosa. However, for colonic mucosal and fecal microbiota, this effect of rifaximin was not obvious. Rifaximin could reshape the correlation of genera between different intestinal segments.
Conclusion
Rifaximin improved visceral hypersensitivity in PI-IBS mice. Rifaximin mainly affected ileal mucosal microbiota, and its improvement effect on IBS might be closely related to the improvement of ileal microbiota structure.
Undernutrition and associated Factors among Pregnant Women in East Borena Zone, Liban District, Oromia regional state, Ethiopia: A community-based cross-sectional study.
Authors: Dassie Godana Arero
Published: July 2022

Background
Undernutrition is “cellular imbalance b/n supply of nutrients, energy and body’s demand to ensure growth, maintenance, and specific function. However, there was no study conducted earlier on this topic in East Borena Zone.
Objective
To assess the prevalence of undernutrition and associated factors among pregnant women in East Borena Zone, Liban district.
Method
A community-based cross-sectional study was conducted on 500 study participants from November 20 to December 2021. The systematic sampling technique and simple random sampling methods were used to select study participants. Data were double entered into Epi-info software version 7 and SPSS version 21 software for analysis. Descriptive statistics were used to describe the characteristics of study participants. Bivariate and multivariable logistic regressions were carried out to identify the association between independent and dependent variables measuring the adjusted odds ratio and 95% confidence interval. P-values less than 0.05 were considered statistically significant.
RESULTS
Prevalence of undernutrition among pregnant women was about (44.9%) family monthly income [AOR = 8.72 (4.80, 15.83)], women decision making autonomy [AOR = 0.40 (0.19, 0.82)], skipping meal [AOR = 2.62 (1.41, 4.89)], substance use [AOR = 2.01 (1.07, 3.77)], household food insecurity [AOR = 2.01 (1.06, 3.80)], lack of prenatal dietary advices [AOR = 2.73 (1.53, 4.89)], absence of household latrine [AOR = 9.23 (3.48, 24.46)], not participating health development army’s meeting at village level [AOR = 3.01 (1.57, 5.72)] and hand washing habit [AOR = 6.55 (3.02, 14.20)] had shown statistically significant association with undernutrition.
Conclusion
The prevalence of undernutrition among pregnant women was high income, women's decision making autonomy, skipping meals, substances use, household food insecurity, lack of prenatal dietary advice, poor hand washing habit, lack of latrine, and not a participation in health development army’s meeting were found to be predictors of the undernutrition.
Mental Health among Children with Long COVID during the COVID- 19 Pandemic
Authors: Shachar-Lavie Iris , Shorer Maayan , Segal Hila , Fennig Silvana , Ashkenazi-Hoffnung Liat
Published: July 2022

Purpose
A growing number of studies report that persons of all ages, infected with SARS-CoV-2, may experience long-term persistent symptoms, known as Long-COVID (LC) or post COVID-19 condition. This is one of the first studies examining the consequences of LC on children’s mental health.
Method
In this case control study, we compared select mental health aspects of 103 children diagnosed with LC to a control group of 113 children uninfected with SARS-COV-2; all 4–18 years old. Both groups were assessed via parents’ questionnaires.
Results
In comparison to the control group, children with LC exhibited more memory difficulties. However, no group differences emerged in other functional aspects (connection with friends and engagement in physical activities), problems with concentration, or levels of emotional-behavioral problems (externalizing, internalizing, ADHD, and PTSD symptoms). We also found that children with LC had greater exposure to COVID 19 related stressors. Higher levels of parental worries regarding their children’s functioning and economic difficulties at home significantly predicted higher levels of children’s emotional-behavioral problems and were better predictors than the child’s age, social functioning, or LC diagnosis.
Conclusions
LC was associated with impairments in some aspects of children’s memory which may relate to academic functioning, but not with higher rates of emotional-behavioral problems, thus warranting interventional programs addressing school functioning and cognitive abilities in this population. Additionally, parents' economic stress and worries regarding their child’s emotional adjustment during the pandemic, are important factors affecting pandemic-related emotional-behavioral problems among children, regardless of COVID-19 infection, that should be addressed.
Design and preparation of MOF@PDA core-shell particles embedded with nano- Ag for PVDF dielectric composites
Authors: Chu Rui , Weng Ling , Guan Lizhu , Li Yuhan , Zhang Xiaorui , Wu Zijian
Published: July 2022

In recent years, dielectric composites as excellent energy storage materials have attracted extensive attention from scholars. Improving the energy storage density of dielectric composites can start from two aspects: increasing the dielectric constant and breakdown field strength of materials. In this paper, the metal-organic framework (MOF) ZIF-67 were selected, the polydopamine (PDA) core shell were coated on the surface of ZIF-67, and nano-Ag were embedded in the PDA, and MOF@PDA-Ag hybrid nanoparticles were prepared. Nanoparticles were introduced into the polyvinylidene fluoride (PVDF) matrix, and MOF@PDA-Ag/PVDF nanocomposites were prepared. The results show that ZIF-67 promote the generation of β-phase in the PVDF matrix, which improve the overall polarization capacity of the composites, thereby improving the mechanical properties and dielectric properties of the materials. Under the strengthening effect of nano-Ag, the dielectric constant of 7 wt% 10MOF@PDA-Ag/PVDF nanocomposite film reaches 15.6 (100 Hz), which is 54.5% higher than that of pure PVDF. Under the influence of the insulation stability of ZIF-67 and PDA and the Coulomb blocking effect of nano-Ag, the breakdown field strength of 5 wt% 10MOF@PDA-Ag/PVDF nanocomposite film is increased to 159kV/mm, which is 62.2% higher than that of pure PVDF.
Development of Functional Cotton Fabric by Simultaneous Dyeing and Finishing with Novel Bioactive Reactive dye
Authors: Nosheen Anum , Ashraf Munir , Hussain Muhammad Tahir , Rehman Abdur , Aziz Humera , Riaz Shagufta , Akram Saba
Published: July 2022

Bioactive textiles have garnered considerable attention during the current pandemic COVID-19 owing to their antimicrobial properties. However, the fabrication and utilization of bioactive textiles have imposed a toxic impact on the environment due to the leaching of bioactive agents during laundering. In this study, a novel functional reactive dye was synthesized with an antibacterial agent covalently linked to the triazine reactive group for simultaneous dyeing and finishing of cotton fabric. The synthesized dye was characterized by FTIR, 1H-NMR, 13C-NMR, and UV-Vis spectroscopy for structure elucidation and confirmation. The dye was applied to cotton fabric using the exhaust dyeing method. The antibacterial efficacy of the powder dye and dyed fabric against Staphylococcus Aureus and Escherichia Coli was estimated according to standard test protocols. The color strength (K/S), colorimetric data (L*, a*, b*, C*, H*) measurement, ultraviolet protection, and colorfastness properties of dyed fabric were also established and evaluated. The synthesized powder dye revealed outstanding antibacterial activity and killed 99.99% of bacteria (E. coli and S. aureus). The dyed fabric showed excellent antibacterial activity before washing and after 20 industrial laundry cycles, there was no significant reduction in antibacterial activity establishing non-leaching of antibacterial agent into environment. The dyed fabrics before and after washing showed excellent ultraviolet protection values (> 50), better dye fixation, and fastness properties. The current study has established a sustainable process to develop bioactive textiles which do not leach bioactive agent in the environment, and it is easily scalable at industrial scale.
Anti-hyperuricemia mechanism of TongFengTangSan (TFTS) by UHPLC-Q-TOF/MS-based nontargeted metabonomics
Authors: Huang Zhichao , Zhang Wugang , An Qiong , Lang Yifan , Liu Ye , Fan Huifang , Chen Haifang
Published: July 2022

Background: TongFengTangSan (TFTS) is a commonly used Tibetan prescription for the treatment of gout. Previously, we confirmed that 90% alcohol extract of TFTS (CF) has a significant uric acid-lowering effect; however, the anti-hyperuricemia mechanisms and main active fractions remain unclear. This study aimed to investigate the anti-hyperuricemia mechanism by metabolomics and confirm the active fraction of CF. Methods: Hyperuricemia model was established by intraperitoneal injection of 100 mg/kg potassium oxonate and 150 mg/kg hypoxanthine by gavage. The serum uric acid (sUA), creatinine (CRE), blood urea nitrogen (BUN), xanthine oxidation (XOD) activity, interleukin-6 (IL-6) and interleukin-1β (IL-1β) were used as indicators to evaluate the efficacy of the CF and four fractions (SX, CF30, CF60, and CF90). The anti-hyperuricemia mechanism of CF in the treatment of hyperuricemia was evaluated through nontargeted metabolomics based on UPLC-Q-TOF-MS technology. Principle component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to explore the potential biomarkers in hyperuricemia, as well as the differential metabolites and metabolic pathways regulated by CF and four fractions.Results: CF showed the significant anti-hyperuricemia effect through the down-regulation on the level of sUA, sCRE, sIL-1β and XOD. SX, CF30, CF60, and CF90 showed the difference on the anti-hyperuricemia effect. Among four fractions, CF60 significantly lowered the level of sUA and could be the main lowering uric acid efficacy fraction of TFTS. 33 differential metabolites were identified in hyperuricemia rats. Pathway analysis revealed five pathways disrupted in hyperuricemia rats. CF reversed 12 endogenous metabolites and the anti-hyperuricemia mechanism of CF could involve in the regulation on purine metabolism and riboflavin metabolism. SX, CF30, CF60, and CF90 regulated 6, 6, 10 and 5 differential metabolites, and involved in differential metabolic pathways, respectively. Among these metabolic pathways, CF60 regulate purine metabolism and riboflavin metabolism. In addition, CF and CF60 could improve intestinal and renal function through the reverse of abnormality of purine metabolism. Conclusions: CF60 could be the main active fraction of CF. CF and CF60 could exert anti-hyperuricemia effect through the regulation on the abnormal purine metabolism caused by hyperuricemia and consequently the improvement of intestinal and renal function.
Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived mesenchymal stem cells via miR-143-3p/ADD3 axis
Authors: Xiong Hewei , Ren Sen , Chen Jing , Yang Xiaofan , Liu Yutian , Xu Zhao , Guo Jiahe , Jiang Tao , Yuan Meng , Liu Yang , Zhang Guolei , Li Wenqing , Machens Hans-Günther , Chen Zhenbing
Published: July 2022

Background
Senescent adipose-derived stem cells (ASCs) exhibit reduced therapeutic efficacy during wound healing. Transcriptional regulation factors including long non-coding RNAs (lncRNAs) reportedly play essential roles in stem cell aging. However, the mechanisms by which lncRNAs influence mesenchymal stem cell aging have not been investigated.
Methods
The lncRNA senescence-associated non-coding RNA (SAN) was identified among differentially expressed lncRNAs in ASCs obtained from old and young volunteer donors. ASCs with overexpression or knockdown of SAN and ADD3 were constructed by lentiviral transduction. Proliferation, migration, and cellular senescence were investigated in ASCs, as were the angiogenic effects on endothelial cells and promigratory effects on fibroblasts stimulated with conditioned medium (CM) from ASCs. Dual-luciferase assays were performed to characterize RNA interactions. The therapeutic effects of SAN-depleted aged ASCs were evaluated in a skin injury model.
Results
The lncRNA SAN (NONHSAT035482.2) was upregulated in aged ASCs and functioned to regulate cellular senescence in ASCs. lncRNA SAN knockdown and ADD3 silencing in ASCs led to ASC functional enhancement and the inhibition of cellular senescence; it also promoted the effects of CM on endothelial cells and fibroblasts. Mechanistic analysis showed that SAN serves as a sponge for miR-143-3p, thereby regulating the expression of γ-adducin (ADD3); moreover, ADD3 silencing could rejuvenate senescent ASCs. The application of SAN-depleted aged ASCs accelerated the skin wound closure, compared with transplantation of aged ASCs.
Conclusion
The lncRNA SAN mediates ASC senescence by regulating the miR-143-3p/ADD3 pathway, providing a potential target for rejuvenation of senescent ASCs and enhancement of wound repair.
Knowledge, attitude and practice of healthcare providers on mistreatment during labour and childbirth: a cross-sectional study in Tehran, Iran, 2021
Authors: Mirzania Marjan , Shakibazadeh Elham , Bohren Meghan A. , Babaey Farah , Hantoushzadeh Sedigheh , Khajavi Abdoljavad , Foroushani Abbas Rahimi
Published: July 2022

Background: Mistreatment of women during childbirth is a global health challenge. Maternity healthcare providers have key roles in influencing women’s birth experiences. This study aimed to assess knowledge, attitudes and practices of maternity healthcare providers about mistreatment during labour and childbirth in public hospitals in Tehran, Iran.Methods: This cross-sectional study was conducted from October to December 2021 in five public hospitals in Tehran. All maternity healthcare providers (obstetricians, midwives) and students were invited to the study. Data were collected using a questionnaire consisting of four sections: socio-demographic characteristics (11 items), knowledge (11 items), attitudes (13 items), and practices (14 items) about mistreatment. Knowledge, attitude, and practice levels were determined using Bloom’s cut off point. Data were analyzed using descriptive and analytical statistics at a significant level of p < 0.05.Results: A total of 255 individuals participated (response rate: 94.5%). Most participants (82.7%) had poor knowledge about physical abuse, verbal abuse, poor rapport between women and providers, and failure to meet professional standards of care. Most participants (69.4%) were alright with physical abuse, verbal abuse, and discrimination. Self-reported practices of different types of mistreatment were not common and only 3.1% of the participants were in moderate level. However, shouted at women, applied fundal pressure, and slapped the thighs during birth were categories used by providers. Age, profession, field of study, employment status, monthly income, work experience, numbers of night shifts, and training history were significantly related with the participants’ knowledge, attitudes, and practices about mistreatment.Conclusions: Knowledge, attitudes, and practices of our participants were poor about maternity mistreatment. Findings of our study have important implications for program planners and decision makers on development of effective interventions to reduce mistreatment during labour and childbirth in Iran. These interventions should include designing and implementing continuing education courses and revising the educational curriculums to increase knowledge, strengthen positive attitudes, and modify practice of maternity healthcare providers, overcoming staff shortages, paying staff fairly, establishing support culture for mother-centered and respectful care, and increasing quality of maternity care.