The Effect of Various Photoperiodic Conditions and Zn2+ Concentrations on Growth Rate and Metabolite Content in Euglena sp.
Authors: Suyono Eko Agus ,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia, Budiman Arief , Ferniah Rejeki Siti , Astiti Adam , Mardyansah Deviko , Natalia Fitri , Cindiati Maya , Maghfiroh Khusnul Qonita , Erfianti Tia , Nurafifah Istini , Amelia Ria , Kurnianto Dedy , Sadewo Brilian Ryan , Maggandari Revata ,   Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Biology, Faculty of Science and Mathematics, Universitas Diponegoro, Semarang 50275, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Research Centre for Food Technology and Processing, National Research and Innovation Agency, Gunung Kid-ul 55811, Indonesia,   Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Sleman 55281, Indonesia,   Department of Mechanical and Industrial Engineering, Faculty of Engineering, Universitas Gadjah Mada, Sleman 55281, Indonesia
Published: July 2024

The application of Euglena as a carbon capture organism has generated considerable interest among scientists. Through the photosynthesis process, many kinds of metabolites are produced by Euglena, such as lipids, proteins, and pigments. Due to the metabolites produced by Euglena, it is vital to optimize the carbon capture ability and cell growth rate by adding Zn2+ content and giving photoperiodic into Euglena culture. The purpose of this study is to identify the optimal photoperiod and Zn2+ concentration to increase the growth rate, biomass, and metabolite content of Euglena sp. This study is a laboratory experiment involving the cultivation of Euglena sp. in various photoperiod cycles (light: dark), namely 24:0, 12:12, 14:10, and 16:8. In addition, Euglena sp. was also cultivated using different concentrations of Zn2+ (0 ppm, 5 ppm, 10 ppm, and 15 ppm). The growth of Euglena sp. was monitored for 18 days before being harvested every three days to measure the research parameters, including primary and secondary metabolites. The results showed that the photoperiod treatment and various concentrations of Zn2+ had a significant impact (P<0.05) on the growth rate, biomass, lipid, carbohydrate, protein, chlorophyll, and carotenoid levels of Euglena sp.