bluuebunny/crossref_metadata_2025 huggingface dataset:
Iron-Coupled Anaerobic Oxidation of Methane in Marine Sediments: A Review
Authors: Yang Hailin , Yu Shan , Lu Hailong
Published: August 2021

Anaerobic oxidation of methane (AOM) is one of the major processes of oxidizing methane in marine sediments. Up to now, extensive studies about AOM coupled to sulfate reduction have been conducted because SO42− is the most abundant electron acceptor in seawater and shallow marine sediments. However, other terminal electron acceptors of AOM, such as NO3−, NO2−, Mn(IV), Fe(III), are more energetically favorable than SO42−. Iron oxides, part of the major components in deep marine sediments, might play a significant role as an electron acceptor in the AOM process, mainly below the sulfate–methane interface, mediated by physiologically related microorganisms. Iron-coupled AOM is possibly the dominant non-sulfate-dependent AOM process to consume methane in marine ecosystems. In this review, the conditions for iron-coupled AOM are summarized, and the forms of iron oxides as electron acceptors and the microbial mechanisms are discussed.
Tocilizumab Induces IL-10-Mediated Immune Tolerance in Invasive Candidiasis
Authors: Tan Zhaohong , Mok Michelle Meng Huang , Mar Soe Win , Thamboo Thomas Paulraj , Goh Jessamine Geraldine , Sam Qi Hui , Osato Motomi , Ravikumar Sharada , Chai Louis Yi Ann
Published: August 2021

The existence of a hyperinflammatory state has been observed in patients with invasive fungal infections (IFI). It is being postulated whether morbidity from IFI may, in part, be a consequence of an unnecessarily prolonged or exaggerated proinflammatory immune response including interleukin 6 (IL-6) post-infection, in a host with dysregulated or compromised immunity. This, in turn, induces collateral host injury at the tissue and organ level, leading to adverse outcomes. Tocilizumab has become widely used as an immunomodulator in the treatment of inflammatory conditions. Here, we evaluated the use of tocilizumab to curb post-infective inflammatory flare in the setting of an in-vivo mouse model for invasive candidiasis. Following Candida infection, the tocilizumab-treated mice showed improved short-term survival compared with the saline-treated control mice. There was a reduced inflammatory response mounted by the host, coupled with reduced IL-6 but increased IL-10 levels. TNF-α and IFN-γ responses were not affected. Tocilizumab facilitated immune tolerance by selectively inducing IL-10, producing CD8α+ conventional dendritic cells (DCs) and peripheral T-regulatory cells, over CD11b+ conventional DCs and plasmacytoid DCs. We demonstrate here the sequelae from immunomodulatory manipulation and the basis whereby the use of monoclonal antibodies may be further explored in IFI.
DPYD Exome, mRNA Expression and Uracil Levels in Early Severe Toxicity to Fluoropyrimidines: An Extreme Phenotype Approach
Authors: Villalvazo Priscila , Marzal-Alfaro Belén , García-Alfonso Pilar , Revuelta-Herrero José Luis , Thomas Fabienne , López-Tarruella Sara , García-González Xandra , Calvo Aitana , Yakoubi Malika , Salvador-Martín Sara , López-López Flora , Aguilar Iker , Sanjurjo-Sáez María , Martín Miguel , López-Fernández Luis Andrés
Published: August 2021

Dihydropyrimidine dehydrogenase deficiency is a major cause of severe fluoropyrimidine-induced toxicity and could lead to interruption of chemotherapy or life-threatening adverse reactions. This study aimed to characterize the DPYD exon sequence, mRNA expression and in vivo DPD activity by plasma uracil concentration. It was carried out in two groups of patients with extreme phenotypes (toxicity versus control) newly treated with a fluoropyrimidine, during the first three cycles of treatment. A novel nonsense gene variant (c.2197insA) was most likely responsible for fluoropyrimidine-induced toxicity in one patient, while neither DPYD mRNA expression nor plasma uracil concentration was globally associated with early toxicity. Our present work may help improve pharmacogenetic testing to avoid severe and undesirable adverse reactions to fluoropyrimidine treatment and it also supports the idea of looking beyond DPYD.
Low-Temperature Gas Plasma Combined with Antibiotics for the Reduction of Methicillin-Resistant Staphylococcus aureus Biofilm Both In Vitro and In Vivo
Authors: Guo Li , Yang Lu , Qi Yu , Niyazi Gulimire , Zheng Jianbao , Xu Ruobing , Chen Xusong , Zhang Jingye , Xi Wang , Liu Dingxin , Wang Xiaohua , Chen Hailan , Kong Michael G.
Published: August 2021

Biofilm infections in wounds seriously delay the healing process, and methicillin-resistant Staphylococcus aureus is a major cause of wound infections. In addition to inactivating micro-organisms, low-temperature gas plasma can restore the sensitivity of pathogenic microbes to antibiotics. However, the combined treatment has not been applied to infectious diseases. In this study, low-temperature gas plasma treatment promoted the effects of different antibiotics on the reduction of S. aureus biofilms in vitro. Low-temperature gas plasma combined with rifampicin also effectively reduced the S. aureus cells in biofilms in the murine wound infection model. The blood and histochemical analysis demonstrated the biosafety of the combined treatment. Our findings demonstrated that low-temperature gas plasma combined with antibiotics is a promising therapeutic strategy for wound infections.
5-(2,6-Diacetamidopyridin-4-yl)-kryptoBODIPY
Authors: Cordaro Massimiliano , Trapani Mariachiara
Published: August 2021

The development of new dyes for various fields of application is of primary interest for the scientific community, among these BODIPY are widely studied for their versatility. This communication describes the synthesis of a BODIPY dye on which a diacetoamidopyridine moiety is connected in meso position. The synthesis procedure requires a one-pot step and the dye is obtained with a yield of 20%. The diacetoamido portion contains chemical functionalities able to favor the interaction of BODIPY with complementary molecules, such as uracil or thymine, offering potential applications for the design of new functional materials or sensors.
Harnessing 3D Printing of Plastics in Construction—Opportunities and Limitations
Authors: Skoratko Aneta , Katzer Jacek
Published: August 2021

Additive manufacturing has been of increasing interest to the construction industry for the last ten years. The subject of the research is the printing of concrete, metals, and plastics. In their analysis and research, authors have focused on printing plastics. 3D printing of reinforcement of concrete elements made of plastics can significantly improve the efficiency of their erection, reduce the amount of waste, and optimize their shape. In this paper, recent developments in the 3D printing of plastics for construction are reviewed. Various applications were discussed, including unconventional spatial reinforcement (impossible to achieve in a traditional way), printed permanent formwork, etc. The challenges for further research and practical applications of such solutions were also discussed.
A System Identification and Implementation of a Soft Sensor for Freeform Bending
Authors: Stebner Sophie Charlotte , Maier Daniel , Ismail Ahmed , Balyan Shubham , Dölz Michael , Lohmann Boris , Volk Wolfram , Münstermann Sebastian
Published: August 2021

The primary goal of this study is the formulation of a soft sensor that predicts industrially relevant mechanical properties for freeform bending. This serves as the foundation of a closed-loop property control. It is hypothesized that by inline measurement of hardness, predictions regarding residual hoop stresses, local strength and strain level can be achieved. A novel hardness-based correlation scheme is introduced, which is implemented into an extended Kalman filter (EKF) and allows an inline prediction of local strength, residual hoop stresses and plasticity. Furthermore, the ultrasonic contact impedance (UCI) method is validated as a suitable inline measuring solution.
Enamel Demineralization Resistance and Remineralization by Various Fluoride-Releasing Dental Restorative Materials
Authors: Kim Min-Ji , Lee Myung-Jin , Kim Kwang-Mahn , Yang Song-Yi , Seo Ji-Young , Choi Sung-Hwan , Kwon Jae-Sung
Published: August 2021

The aim of this study is to investigate the resistance of various fluoride-releasing restorative materials against the demineralization and remineralization of enamel surfaces, including those that have been recently introduced to the market. Three different fluoride-releasing restorative materials were considered: glass ionomer (FI), resin-modified glass ionomer (RL), and an alkasite restorative material (CN). The acid neutralization ability was investigated using pH measurement, and the concentrations of released fluoride and calcium ions were measured. Finally, the demineralization resistance and remineralization effects of enamel were observed using a microhardness tester and SEM. CN showed an initial substantial increase in pH followed by a steady increase, with values higher than those of the other groups (p < 0.05). All three groups released fluoride ions, and the CN group released more calcium ions than the other groups (p < 0.05). In the acid resistance test, from the microhardness and SEM images, the CN group showed effective resistance to demineralization. In the remineralization test, the microhardness results showed that the FI and CN groups recovered the microhardness from the values of the demineralized enamel surface (p < 0.05). This was confirmed by the SEM images from remineralization tests; the CN group showed a recovered demineralized surface when immersed in artificial saliva for 7 days. In conclusion, alkasite restorative material can be an effective material when used in cariogenic environments.
The Comparative Assessment of Effects on the Power System and Environment of Selected Electric Transport Means in Poland
Authors: Markowska Katarzyna , Flizikowski Józef , Bieliński Kazimierz , Tomporowski Andrzej , Kruszelnicka Weronika , Kasner Robert , Bałdowska-Witos Patrycja , Mazur Łukasz
Published: August 2021

Currently, electric vehicles are a rapidly growing alternative to those with combustion engines and can contribute to reduction of CO2 emissions in the transport sector, especially when the energy to power electric motors is predominantly derived from renewable sources. Until now, the comparison of environmental impact and influence of electric transport means on the power systems was not fully addressed in the case of Poland. The purpose of the study is to describe, analyse and assess electric vehicles (EV) operation against performance indicators in Poland, especially the influence of electric transport means (ETM) (electric cars, trams, trolley buses and buses) on power system and environment. The influence on the power system was investigated for the Polish National Powers system using the simulation of different scenarios of loads generated by EV charging. The energy demand of the National Power System and daily load variability indices were determined. Based on the data of ETM powers consumption and emissions of energy production, the emissions of harmful gases per one km and per one person were calculated, as well as the financial outlays for energy necessary to drive 1 km per 1 passenger. To assess and compare the environmental impact of the selected ETM life cycle, the life cycle assessment method was used. The results of environmental impacts were determined for selected assessment methods: CML 2 and IPCC 2013 GWP 100. The functional unit in this study is one selected ETM with a service life of 100,000 km. Comparison of trams, trolley buses, buses and electric passenger cars indicates that most beneficial are electric buses which do not need rails or overhead lines, thus investment costs are lower.
New Insights on the Vibrational Dynamics of 2-Methoxy-, 4-Methoxy- and 4-Ethoxy-Benzaldehyde from INS Spectra and Periodic DFT Calculations
Authors: Ribeiro-Claro Paulo J. A. , Vaz Pedro D. , Nolasco Mariela M. , Gil Francisco P. S. C. , de Carvalho Luís A. E. Batista , Marques Maria Paula M. , Amado Ana M.
Published: August 2021

The dynamics of 2-methoxybenzaldehyde, 4-methoxybenzaldehyde, and 4-ethoxybenzaldehyde in the solid state are assessed through INS spectroscopy combined with periodic DFT calculations. In the absence of experimental data for 4-ethoxybenzaldehyde, a tentative crystal structure, based on its similarity with 4-methoxybenzaldehyde, is considered and evaluated. The excellent agreement between calculated and experimental spectra allows a confident assignment of the vibrational modes. Several spectral features in the INS spectra are unambiguously assigned and torsional potential barriers for the methyl groups are derived from experimental frequencies. The intramolecular nature of the potential energy barrier for methyl rotation about O–CH3 bonds compares with the one reported for torsion about saturated C–CH3 bonds. On the other hand, the intermolecular contribution to the potential energy barrier may represent 1/3 of the barrier height in these systems.
Adaptive Data-Driven Control for Linear Time Varying Systems
Authors: Abdalla Talal
Published: August 2021

In this paper, we propose an adaptive data-driven control approach for linear time varying systems, affected by bounded measurement noise. The plant to be controlled is assumed to be unknown, and no information in regard to its time varying behaviour is exploited. First, using set-membership identification techniques, we formulate the controller design problem through a model-matching scheme, i.e., designing a controller such that the closed-loop behaviour matches that of a given reference model. The problem is then reformulated as to derive a controller that corresponds to the minimum variation bounding its parameters. Finally, a convex relaxation approach is proposed to solve the formulated controller design problem by means of linear programming. The effectiveness of the proposed scheme is demonstrated by means of two simulation examples.
On the Parity of the Order of Appearance in the Fibonacci Sequence
Authors: Trojovský Pavel
Published: August 2021

Let (Fn)n≥0 be the Fibonacci sequence. The order of appearance function (in the Fibonacci sequence) z:Z≥1→Z≥1 is defined as z(n):=min{k≥1:Fk≡0(modn)}. In this paper, among other things, we prove that z(n) is an even number for almost all positive integers n (i.e., the set of such n has natural density equal to 1).
Fast Terminal Sliding Control of Underactuated Robotic Systems Based on Disturbance Observer with Experimental Validation
Authors: Rojsiraphisal Thaned , Mobayen Saleh , Asad Jihad H. , Vu Mai The , Chang Arthur , Puangmalai Jirapong
Published: August 2021

In this study, a novel fast terminal sliding mode control technique based on the disturbance observer is recommended for the stabilization of underactuated robotic systems. The finite time disturbance observer is employed to estimate the exterior disturbances of the system and develop the finite time control law. The proposed controller can regulate the state trajectories of the underactuated systems to the origin within a finite time in the existence of external disturbances. The stability analysis of the proposed control scheme is verified via the Lyapunov stabilization theory. The designed control law is enough to drive a switching surface achieving the fast terminal sliding mode against severe model nonlinearities with large parametric uncertainties and external disturbances. Illustrative simulation results and experimental validations on a cart-inverted pendulum system are provided to display the success and efficacy of the offered method.
Supported MXene/GO Composite Membranes with Suppressed Swelling for Metal Ion Sieving
Authors: Yin Zongjie , Lu Zong , Xu Yanyan , Zhang Yonghong , He Liliang , Li Peishan , Xiong Lei , Ding Li , Wei Yanying , Wang Haihui
Published: August 2021

Novel two-dimensional (2D) membranes have been utilized in water purification or seawater desalination due to their highly designable structure. However, they usually suffer from swelling problems when immersed in solution, which limits their further applications. In this study, 2D cross-linked MXene/GO composite membranes supported on porous polyamide substrates are proposed to improve the antiswelling property and enhance the ion-sieving performance. Transition-metal carbide (MXene) nanosheets were intercalated into GO nanosheets, where the carboxyl groups of GO combined the neighboring hydroxyl terminal groups of MXene with the formation of -COO- bonds between GO and MXene nanosheets via the cross-linking reaction (−OH + −COOH = −COO− + H2O) after heat treatment. The permeation rates of the metal ions (Li+, Na+, K+, Al3+) through the cross-linked MXene/GO composite membrane were 7–40 times lower than those through the pristine MXene/GO membrane. In addition, the cross-linked MXene/GO composite membrane showed excellent Na+ rejection performance (99.3%), which was significantly higher than that through pristine MXene/GO composite membranes (80.8%), showing improved ion exclusion performance. Such a strategy represents a new avenue to develop 2D material-derived high-performance membranes for water purification.
Study on Deposition Characteristics of Microparticles in Terminal Pulmonary Acini by IB–LBM
Authors: Xu Du-Chang , Luo Yu-Xiao , Xu Yuan-Qing
Published: August 2021

As an indicator of health risk, the deposition of microparticles in terminal pulmonary acini is of great significance in the medical field. To control particulate pollution and optimize aerosol delivery, it is necessary to perform an in-depth study of the microparticle deposition in terminal pulmonary acini; however, little research has been done on this topic. This paper proposes a respiratory movement model of terminal pulmonary acini using an immersed boundary–lattice Boltzmann method. In addition, we explored the effect of gravity direction, respiratory rate, microparticle diameter, and other parameters on the microparticles deposition process and distribution, under the airflow in the acinar wall. It was found that the deposition of microparticles is sensitive to gravity direction, and the growth of the respiratory rate increases the rate of microparticle migration and deposition. It was observed that the gravity effect is enhanced by increasing the diameter of microparticles, causing a high deposition and dispersion rate. The study reveals the dynamic correlation between the respiration process and the movement of microparticles, which is of reference value to figure out the pathogenicity mechanism of inhalable particles and to optimize the aerosol delivery.
Assessment of Phenotype Relevant Amino Acid Residues in TEM-β-Lactamases by Mathematical Modelling and Experimental Approval
Authors: Madzgalla Sara , Duering Helena , Hey Jana C. , Neubauer Svetlana , Feller Karl-Heinz , Ehricht Ralf , Pletz Mathias W. , Makarewicz Oliwia
Published: August 2021

Single substitutions or combinations of them alter the hydrolytic activity towards specific β-lactam-antibiotics and β-lactamase inhibitors of TEM-β-lactamases. The sequences and phenotypic classification of allelic TEM variants, as provided by the NCBI National Database of Antibiotic Resistant Organisms, does not attribute phenotypes to all variants. Some entries are doubtful as the data assessment differs strongly between the studies or no data on the methodology are provided at all. This complicates mathematical and bioinformatic predictions of phenotypes that rely on the database. The present work aimed to prove the role of specific substitutions on the resistance phenotype of TEM variants in, to our knowledge, the most extensive mutagenesis study. In parallel, the predictive power of extrapolation algorithms was assessed. Most well-known substitutions with direct impact on the phenotype could be reproduced, both mathematically and experimentally. Most discrepancies were found for supportive substitutions, where some resulted in antagonistic effects in contrast to previously described synergism. The mathematical modelling proved to predict the strongest phenotype-relevant substitutions accurately but showed difficulties in identifying less prevalent but still phenotype transforming ones. In general, mutations increasing cephalosporin resistance resulted in increased sensitivity to β-lactamase inhibitors and vice versa. Combining substitutions related to cephalosporin and β-lactamase inhibitor resistance in almost all cases increased BLI susceptibility, indicating the rarity of the combined phenotype.
Molecule Diffusion Behavior of Tritium and Selenium in Mongolia Clay Rock by Numerical Analysis of the Spatial and Temporal Variation
Authors: Lee Chuan-Pin , Hu Yanqin , Tien Neng-Chuan , Tsai Shih-Chin , Shi Yunfeng , Liu Weigang , Kong Jie , Sun Yuzhen
Published: August 2021

Clay rock in the Tamusu (TMS) area in the Inner Mongolia province is one candidate as a geological disposal site for highly radioactive waste in China. The diffusion behavior of HTO and Se(IV) in Tamusu clay rock was studied by through-diffusion (TD) experiments and numerical analysis to determine the spatial and temporal variation. A minimum error analysis was conducted to determine the HTO and Se(IV) diffusion coefficients in compacted TMS clay. The TD experimental results and numerical analysis showed that the diffusion of HTO and Se reached a steady state within 7 and 50 days, respectively, and the apparent diffusion coefficients (Da) decreased with the increases in the compacted density. In fact, there was retardation of Se diffusion in intact TMS clay rock. A two-site sorption model for Se was applied to simulate fast and slow sorption behavior quantitatively.
Calorimetric Evaluation of Glycyrrhetic Acid (GA)- and Stearyl Glycyrrhetinate (SG)-Loaded Solid Lipid Nanoparticle Interactions with a Model Biomembrane
Authors: Santonocito Debora , Puglia Carmelo , Torrisi Cristina , Giuffrida Alessandro , Greco Valentina , Castelli Francesco , Sarpietro Maria Grazia
Published: August 2021

Glycyrrhetic acid (GA) and stearyl glycyrrhetinate (SG) are two interesting compounds from Glycyrrhiza glabra, showing numerous biological properties widely applied in the pharmaceutical and cosmetic fields. Despite these appreciable benefits, their potential therapeutic properties are strongly compromised due to unfavourable physical-chemical features. The strategy exploited in the present work was to develop solid lipid nanoparticles (SLNs) as carrier systems for GA and SG delivery. Both formulations loaded with GA and SG (GA-SLNs and SG-SLNs, respectively) were prepared by the high shear homogenization coupled to ultrasound (HSH-US) method, and we obtained good technological parameters. DSC was used to evaluate their thermotropic behaviour and ability to act as carriers for GA and SG. The study was conducted by means of a biomembrane model (multilamellar vesicles; MLVs) that simulated the interaction of the carriers with the cellular membrane. Unloaded and loaded SLNs were incubated with the biomembranes, and their interactions were evaluated over time through variations in their calorimetric curves. The results of these studies indicated that GA and SG interact differently with MLVs and SLNs; the interactions of SG-SLNs and GA-SLNs with the biomembrane model showed different variations of the MLVs calorimetric curve and suggest the potential use of SLNs as delivery systems for GA.
1H-NMR Metabolic Profiling and Antioxidant Activity of Saffron (Crocus sativus) Cultivated in Lebanon
Authors: Samaha Hala , Chahine Nathalie , Sobolev Anatoly Petrovich , Menghini Luigi , Makhlouf Hassane
Published: August 2021

Despite the beneficial health properties shown by Lebanese saffron, its qualitative and quantitative composition has never been investigated before. In the present study, NMR spectroscopy, together with antioxidant activity assays, were applied to evaluate the chemical composition of saffron samples of different geographical origins (Lebanon, Italy, Iran, and India) and to categorize the Lebanese saffron for the first time. The distinction between Lebanese saffron and that produced in other countries was attributed to its higher linolenic and linoleic fatty acids, glucose and picrocrocin contents. Moreover, spices produced in three different regions of the Lebanese territory have been clearly differentiated. Saffron cultivated in the Qaa region displayed a high glucose, fatty acids and polyphenols content, whereas Hermel saffron exhibited the largest rate of picrocrocin and glycosylated carotenoids. Finally, samples from Baalbeck showed lower rates for the majority of metabolites. Moreover, Lebanese saffron showed a high antioxidant activity in ABTS and DPPH assays. A low dose of saffron extract (10 µg/mL) inhibited the growth of human lung adenocarcinoma cells, probably due to the high polyphenolic content. This study highlights the quality and peculiarity of Lebanese saffron cultivated in Northern Beqaa district and allows for a good discrimination between spices produced in relatively close territory.
Saccharpiscinols A–C: Flavans with Potential Anti-Inflammatory Activities from One Actinobacteria Saccharomonospora piscinae
Authors: Su Yung-Shun , Chen Jih-Jung , Cheng Ming-Jen , Chai Chee-Yin , Kwan Aij-Lie , Huang Jheng-Cian , Kuo Yueh-Hsiung
Published: August 2021

Phytochemical investigation and chromatographic separation of extracts from the actinobacteria strain Saccharomonospora piscinae that was isolated from dried fishpond sediment of Kouhu township, in the south of Taiwan, led to the isolation of three new compounds, saccharpiscinols A–C (1–3, respectively), and three new natural products, namely (2S)-5,7,3′,4′-tetrahydroxy-6,8-dimethylflavanone (4), methyl-4-hydroxy-2-methoxy-6-methylbenzoate (5), and (±)-7-acetyl-4,8-dihydroxy-6-methyl-1-tetralone (6). Compounds 4–6 were reported before as synthesized products, herein, they are reported from nature for the first time. The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic data analysis (1D- and 2D-NMR, MS, and UV) and comparison with literature data. The effect of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages was evaluated. Saccharpiscinol A showed inhibitory activities against LPS-induced NO production.
Cytotoxic Evaluation and Determination of Organic and Inorganic Eluates from Restorative Materials
Authors: Roussou Konstantina , Nikolaidis Alexandros K. , Ziouti Fani , Arhakis Aristidis , Arapostathis Konstantinos , Koulaouzidou Elisabeth A.
Published: August 2021

Over the last years, diverse commercial resin-based composites have dominated as dental filling materials. The purpose of the present study was to determine organic and inorganic eluates from five restorative materials using GC/MS and ICP–OES and to compare the effect on cell survival of human gingival fibroblasts of a conventional and a bioactive resin. Five commercially available restorative materials were employed for this study: ActivaTM Bioactive Restorative, ENA HRi, Enamel plus HRi Biofunction, Fuji II LC Capsule, and Fuji IX Capsule. Disks that were polymerized with a curing LED light or left to set were immersed in: 1 mL methanol or artificial saliva for GC/MS analysis, 5mL deionized water for ICP–OES, and 5mL of culture medium for cell viability. Cell viability was investigated with a modified staining sulforhodamine B assay.The following organic substances were detected: ACP, BHT, BPA, 1,4-BDDMA, CQ, DBP, DMABEE, HEMA, MCE, MeHQ, MOPA, MS, TMPTMA, and TPSb and the ions silicon, aluminum, calcium, sodium, and barium. Activa Bioactive Restorative was found to be biocompatible. Elution of organic substances depended on material’s composition, the nature of the solvent and the storage time. Ions’ release depended on material’s composition and storage time. The newly introduced bioactive restorative was found to be more biocompatible.
Morphological Changes and Component Characterization of Coffee Silverskin
Authors: Wang Xia , Hong De-Fu , Hu Gui-Lin , Li Zhong-Rong , Peng Xing-Rong , Shi Qiang-Qiang , Qiu Ming-Hua
Published: August 2021

Nuclear magnetic resonance (NMR) spectroscopy was used for the qualitative and quantitative analysis of aqueous extracts of unroasted and roasted coffee silverskin (CS). Twenty compounds were identified from 1D and 2D NMR spectra, including caffeine, chlorogenic acid (CGA), trigonelline, fructose, glucose, sucrose, etc. For the first time, the presence of trigonelline was detected in CS. Results of the quantitative analysis showed that the total amount of the main components after roasting was reduced by 45.6% compared with values before roasting. Sugars in the water extracts were the main components in CS, and fructose was the most abundant sugar, its relative content accounting for 38.7% and 38.4% in unroasted and roasted CS, respectively. Moreover, 1D NMR combined with 2D NMR technology shows application prospects in the rapid, non-destructive detection of CS. In addition, it was observed by optical microscopy and scanning electron microscopy (SEM) that the morphology of CS changed obviously before and after roasting.
Triple-Band Anisotropic Perfect Absorbers Based on α-Phase MoO3 Metamaterials in Visible Frequencies
Authors: Tang Bin , Yang Neigang , Song Xianglian , Jin Gui , Su Jiangbin
Published: August 2021

Anisotropic materials provide a new platform for building diverse polarization-dependent optical devices. Two-dimensional α-phase molybdenum trioxides (α-MoO3), as newly emerging natural van der Waals materials, have attracted significant attention due to their unique anisotropy. In this work, we theoretically propose an anisotropic perfect metamaterial absorber in visible frequencies, the unit cell of which consists of a multi-layered α-MoO3 nanoribbon/dielectric structure stacked on a silver substrate. Additionally, the number of perfect absorption bands is closely related to the α-MoO3 nanoribbon/dielectric layers. When the proposed absorber is composed of three α-MoO3 nanoribbon/dielectric layers, electromagnetic simulations show that triple-band perfect absorption can be achieved for polarization along [100], and [001] in the direction of, α-MoO3, respectively. Moreover, the calculation results obtained by the finite-difference time-domain (FDTD) method are consistent with the effective impedance of the designed absorber. The physical mechanism of multi-band perfect absorption can be attributed to resonant grating modes and the interference effect of Fabry–Pérot cavity modes. In addition, the absorption spectra of the proposed structure, as a function of wavelength and the related geometrical parameters, have been calculated and analyzed in detail. Our proposed absorber may have potential applications in spectral imaging, photo-detectors, sensors, etc.
The Other Side of Malnutrition in Inflammatory Bowel Disease (IBD): Non-Alcoholic Fatty Liver Disease
Authors: Gibiino Giulia , Sartini Alessandro , Gitto Stefano , Binda Cecilia , Sbrancia Monica , Coluccio Chiara , Sambri Vittorio , Fabbri Carlo
Published: August 2021

Steatohepatitis and hepatobiliary manifestations constitute some of the most common extra-intestinal manifestations of Inflammatory Bowel Disease (IBD). On the other hand, non-alcoholic fatty liver disease (NAFLD) affects around 25% of the world’s population and is attracting ever more attention in liver transplant programs. To outline the specific pathways linking these two conditions is a pressing task for 21st-century researchers. We are accustomed to expecting the occurrence of fatty liver disease in obese people, but current evidence suggests that there are several different pathways also occurring in underweight patients. Genetic factors, inflammatory signals and microbiota are key players that could help in understanding the entire pathogenesis of NAFLD, with the aim of defining the multiple expressions of malnutrition. In the current review, we summarize the most recent literature regarding the epidemiology, pathogenesis and future directions for the management of NAFLD in patients affected by IBD.
The Functional DNA Methylation Signatures Relevant to Altered Immune Response of Neonatal T Cells with l-Arginine Supplementation
Authors: Yu Hong-Ren , Hsu Te-Yao , Tsai Ching-Chang , Huang Hsin-Chun , Cheng Hsin-Hsin , Lai Yun-Ju , Lin Yu-Ju , Chen Chih-Cheng , Li Sung-Chou , Yang Kuender
Published: August 2021

l-Arginine is an important nutrient in the infant diet that significantly regulates the maturation of the immune system in neonates, including the maturation of CD4+ T cells. The biological activities of CD4+ T cells differ substantially between neonates and adults, and these differences may be governed by epigenetic processes. Investigating these differences and the causative processes may help understand neonatal and developmental immunity. In this study, we compared the functional DNA methylation profiles in CD4+ T cells of neonates and adults, focusing on the role of l-arginine supplementation. Umbilical cord blood and adult CD4+ T cells were cultured with/without l-arginine treatment. By comparing DNA methylation in samples without l-arginine treatment, we found that CD4+ T cells of neonatal cord blood generally showed higher DNA methylation than those of adults (average CpG methylation percentage 0.6305 for neonate and 0.6254 for adult, t-test p-value < 0.0001), suggesting gene silencing in neonates. By examining DNA methylation patterns of CpG dinucleotides induced by l-arginine treatment, we found that more CpG dinucleotides were hypomethylated and more genes appeared to be activated in neonatal T-cells as compared with adult. Genes activated by l-arginine stimulation of cord blood samples were more enriched regarding immune-related pathways. CpG dinucleotides at IL-13 promoter regions were hypomethylated after l-arginine stimulation. Hypomethylated CpG dinucleotides corresponded to higher IL-13 gene expression and cytokine production. Thus, DNA methylation partially accounts for the mechanism underlying differential immune function in neonates. Modulatory effects of l-arginine on DNA methylation are gene-specific. Nutritional intervention is a potential strategy to modulate immune function of neonates.
Unveiling the Different Reactivity of Bent and Linear Three-Atom-Components Participating in [3 + 2] Cycloaddition Reactions
Authors: Ríos-Gutiérrez Mar , Domingo Luis , Ghodsi Fatemeh
Published: August 2021

The reactivity of a series of pairs of bent and linear three-atom-component (B-TACs and L-TACs) participating in [3 + 2] cycloaddition (32CA) reactions towards ethylene and electrophilic dicyanoethylene (DCE) have been studied within the Molecular Electron Density Theory. While the pseudodiradical structure of B-TACs changes to that of pseudoradical or carbenoid L-TACs upon dehydrogenation, zwitterionic B-TACs remain unchanged. Conceptual Density Functional Theory (CDFT) indices characterize five of the nine TACs as strong nucleophiles participating in polar reactions towards electrophilic ethylenes. The activation energies of the 32CA reactions with electrophilic DCE range from 0.5 to 22.0 kcal·mol−1, being between 4.3 and 9.1 kcal·mol−1 lower than those with ethylene. In general, B-TACs are more reactive than their L-TAC counterparts. A change in the regioselectivity is found in these polar 32CA reactions; in general, while B-TACs are meta regioselective, L-TACs are ortho regioselective. The geometrical parameters of the transition state structures suggest that the formation of the single bond involving the most electrophilic carbon of DCE is more advanced. A change in the asynchronicity in the reactions involving B-TACs and L-TACs is also found.
Increasing Public Health Mosquito Surveillance in Hidalgo County, Texas to Monitor Vector and Arboviral Presence
Authors: Guerrero Clarissa D. , Hinojosa Steven , Vanegas Diana , Tapangan Niko , Guajardo Matthew , Alaniz Sara , Cano Narda , Vitek Christopher J. , Thomas John , Hernandez Valerie , Garcia Juan , Bolling Bethany G. , Qualls Whitney A. , Tyler Ronald , Olivarez Eduardo
Published: August 2021

From 2016 to 2018, Hidalgo County observed the emergence of Zika virus (ZIKV) infections along with sporadic cases of Dengue virus (DENV) and West Nile virus (WNV). Due to the emergence of ZIKV and the historical presence of other mosquito-borne illnesses, Hidalgo County obtained funding to enhance mosquito surveillance and educate residents on arboviruses and travel risks. During this time period, Hidalgo County mosquito surveillance efforts increased by 1.275%. This increase resulted in >8000 mosquitoes collected, and 28 mosquito species identified. Aedes aegypti, Ae albopictus and Culex quinquefasciatus made up approximately two-thirds of the mosquitoes collected in 2018 (4122/6171). Spatiotemporal shifts in vector species composition were observed as the collection period progressed. Significantly, temperature variations (p < 0.05) accounted for associated variations in vector abundance, whereas all other climate variables were not significant.
Magnetoliposomes Based on Shape Anisotropic Calcium/Magnesium Ferrite Nanoparticles as Nanocarriers for Doxorubicin
Authors: Cardoso Beatriz D. , Rodrigues Ana Rita O. , Bañobre-López Manuel , Almeida Bernardo G. , Amorim Carlos O. , Amaral Vítor S. , Coutinho Paulo J. G. , Castanheira Elisabete M. S.
Published: August 2021

Multifunctional lipid nanocarriers are a promising therapeutic approach for controlled drug release in cancer therapy. Combining the widely used liposome structure with magnetic nanoparticles in magnetoliposomes allies, the advantages of using liposomes include the possibility to magnetically guide, selectively accumulate, and magnetically control the release of drugs on target. The effectiveness of these nanosystems is intrinsically related to the individual characteristics of the two main components—lipid formulation and magnetic nanoparticles—and their physicochemical combination. Herein, shape-anisotropic calcium-substituted magnesium ferrite nanoparticles (Ca0.25Mg0.75Fe2O4) were prepared for the first time, improving the magnetic properties of spherical counterparts. The nanoparticles revealed a superparamagnetic behavior, high saturation magnetization (50.07 emu/g at 300 K), and a large heating capacity. Furthermore, a new method for the synthesis of solid magnetoliposomes (SMLs) was developed to enhance their magnetic response. The manufacturing technicalities were optimized with different lipid compositions (DPPC, DPPC/Ch, and DPPC/DSPE-PEG) originating nanosystems with optimal sizes for biomedical applications (around or below 150 nm) and low polydispersity index. The high encapsulation efficiency of doxorubicin in these magnetoliposomes was proven, as well as the ability of the drug-loaded nanosystems to interact with cell membrane models and release DOX by fusion. SMLs revealed to reduce doxorubicin interaction with human serum albumin, contributing to a prolonged bioavailability of the drug upon systemic administration. Finally, the drug release kinetic assays revealed a preferable DOX release at hyperthermia temperatures (42 °C) and acidic conditions (pH = 5.5), indicating them as promising controlled release nanocarriers by either internal (pH) and external (alternate magnetic field) stimuli in cancer therapy.
Modification of Proliferation and Apoptosis in Breast Cancer Cells by Exposure of Antioxidant Nanoparticles Due to Modulation of the Cellular Redox State Induced by Doxorubicin Exposure
Authors: López-Barrera Laura Denise , Díaz-Torres Roberto , Martínez-Rosas Joselo Ramón , Salazar Ana María , Rosales Carlos , Ramírez-Noguera Patricia
Published: August 2021

In this report, we investigated whether the use of chitosan-carrying-glutathione nanoparticles (CH-GSH NPs) can modify proliferation and apoptosis, and reduce cell damage induced by doxorubicin on breast cancer cells. Doxorubicin is a widely used antineoplasic agent for the treatment of various types of cancer. However, it is also a highly toxic drug because it induces oxidative stress. Thus, the use of antioxidant molecules has been considered to reduce the toxicity of doxorubicin. CH-GSH NPs were characterized in size, zeta potential, concentration, and shape. When breast cancer cells were treated with CH-GSH nanoparticles, they were localized in the cellular cytoplasm. Combined doxorubicin exposure with nanoparticles increased intracellular GSH levels. At the same time, decreasing levels of reactive oxygen species and malondialdehyde were observed and modified antioxidant enzyme activity. Levels of the Ki67 protein were evaluated as a marker of cell proliferation and the activity of the Casp-3 protein related to cell apoptosis was measured. Our data suggests that CH-GSH NPs can modify cell proliferation by decreasing Ki67 levels, induce apoptosis by increasing caspase-3 activity, and reduce the oxidative stress induced by doxorubicin in breast cancer cells by modulating molecules associated with the cellular redox state. CH-GSH NPs could be used to reduce the toxic effects of this antineoplastic. Considering these results, CH-GSH NPs represent a novel delivery system offering new opportunities in pharmacy, material science, and biomedicine.
Miniaturized Interferometric Sensors with Spectral Tunability for Optical Fiber Technology—A Comparison of Size Requirements, Performance, and New Concepts
Authors: Hillmer Hartmut , Woidt Carsten , Kobylinskiy Aliaksei , Kraus Matthias , Istock André , Iskhandar Mustaqim S. Q. , Brunner Robert , Kusserow Thomas
Published: August 2021

Optical interferometric sensors have acquired significant importance in metrology and information technology, especially in terms of their potential application in launching size, selectivity, sensitivity, resolution, spectral tuning ranges, efficiency, and cost. However, these demands are often contradictory and counteract one another, and are thus difficult to simultaneously fulfill during their interaction. This review focuses on a detailed comparison of seven different strongly miniaturized sensor concepts investigating the limits of these demands. For the visible and near-infrared spectral range, seven optical sensors were reviewed based on the following methodologies: classical optical transmission and reflection gratings, arrayed waveguide gratings, static Fabry–Pérot (FP) filter arrays, MEMS tunable FP interferometers, MEMS tunable photonic crystals, plasmonic filters, and fiber tip sensors. The comparison between the selected concepts concentrates on (i) the minimum space required for a particular spectral range, (ii) resolution, (iii) the integration in optical fiber technology, (iv) tunability to save space, (v) efficiency in using available light, (vi) multiplexing, (vii) miniaturization limits, and (viii) the potential of nanoimprint for cost reduction. Technologies for enhancing efficiency to obtain more available light and their applicability to the different methodologies were studied.
Caribbean-Wide, Negative Emissions Solution to Sargassum spp. Low-Cost Collection Device and Sustainable Disposal Method
Authors: Gray Luke A. , Bisonó León Andres G. , Rojas Folkers E. , Veroneau Samuel S. , Slocum Alexander H.
Published: August 2021

Sargassum spp. blooms exacerbated by climate change and agricultural runoff are inundating Caribbean beaches, emitting toxic fumes and greenhouse gases through decomposition. This hurts tourism, artisanal fishing, shore-based industry, human health, standards-of-living, coastal ecology, and the global climate. Barriers, collection machinery, and Sargassum valorization have been unable to provide sufficient, sustainable, or widespread relief. This article presents a total Sargassum management system that is effective, low-impact, and economically scalable across the Caribbean. Littoral Collection Modules (LCMs), attached to artisanal fishing boats, collect Sargassum in nets which are brought to a barge. When full, the barge is towed to the deep ocean where Sargassum is pumped to ~150–200 m depth, whereafter it continues sinking (Sargassum Ocean Sequestration of Carbon; “SOS Carbon”). Costing and negative emissions calculations for this system show cleanup costs <$1/m3 and emissions reduction potential up to 1.356 → 3.029 tCO2e/dmt Sargassum. COVID-19 decimated Caribbean tourism, adding to the pressures of indebtedness and natural disasters facing the region. The “SOS Carbon strategy” could help the Caribbean “build back better” by establishing a negative emissions industry that builds resilience against Sargassum and flight shame (“flygskam”). Employing fishermen to operate LCMs achieves socioeconomic goals while increasing Sargassum cleanup and avoiding landfilling achieves sustainable development goals.
Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa)
Authors: Louati Marwa , Salazar-Sarasua Blanca , Roque Edelín , Beltrán José Pío , Salhi Hannachi Amel , Gómez-Mena Concepción
Published: August 2021

Argan trees (Argania spinosa) belong to a species native to southwestern Morocco, playing an important role in the environment and local economy. Argan oil extracted from kernels has a unique composition and properties. Argan trees were introduced in Tunisia, where hundreds of trees can be found nowadays. In this study, we examined reproductive development in Argan trees from four sites in Tunisia and carried out the functional characterization of a floral homeotic gene in this non-model species. Despite the importance of reproductive development, nothing is known about the genetic network controlling flower development in Argania spinosa. Results obtained in several plant species established that floral organ development is mostly controlled by MADS-box genes and, in particular, APETALA3 (AP3) and PISTILLATA (PI) homologs are required for proper petal and stamen identity. Here, we describe the isolation and functional characterization of a MADS-box gene from Argania spinosa. Phylogenetic analyses showed strong homology with PI-like proteins, and the expression of the gene was found to be restricted to the second and third whorls. Functional homology with Arabidopsis PI was demonstrated by the ability of AsPI to confer petal and stamen identity when overexpressed in a pi-1 mutant background. The identification and characterization of this gene support the strong conservation of PI homologs among distant angiosperm plants.
Surface Modification of Cellulose Nanocrystals with Lactone Monomers via Plasma-Induced Polymerization and Their Application in ABS Nanocomposites
Authors: Díaz de León Ramón , Guzmán Ediberto , López González Ricardo , Díaz Elizondo Alejandro , Magaña Ilse , Neira Guadalupe , Castañeda Facio Adali , Valencia Luis
Published: August 2021

The growing concern for environmental problems has motivated the use of materials obtained from bio-based resources such as cellulose nanocrystals which have a promising application acting as fillers or reinforcements of polymeric materials. In this context, in this article, plasma-induced polymerization is proposed as a strategy to modify nanocrystals at different plasma power intensities using ε-caprolactone and δ-decalactone to improve their compatibility with polymeric matrices. The characterization was carried out using techniques such as FTIR, TGA, XRD, XPS, and AFM, with which a successful functionalization was demonstrated without altering the inherent properties of the nanocrystals. The preparation of ABS nanocomposites was carried out with the modified nanoparticles and the evaluation of the mechanical properties indicates an increase in Young’s modulus and yield stress under certain concentrations of modified cellulose nanocrystals.