bluuebunny/crossref_metadata_2025 huggingface dataset:
Longitudinal analysis of epigenome-wide DNA methylation reveals novel loci associated with BMI change in East Asians
Authors: Li Wenran , Xia Mingfeng , Zeng Hailuan , Lin Huandong , Teschendorff Andrew E. , Gao Xin , Wang Sijia
Published: May 2024

Background

Obesity is a global public health concern linked to chronic diseases such as cardiovascular disease and type 2 diabetes (T2D). Emerging evidence suggests that epigenetic modifications, particularly DNA methylation, may contribute to obesity. However, the molecular mechanism underlying the longitudinal change of BMI has not been well-explored, especially in East Asian populations.

Methods

This study performed a longitudinal epigenome-wide association analysis of DNA methylation to uncover novel loci associated with BMI change in 533 individuals across two Chinese cohorts with repeated DNA methylation and BMI measurements over four years.

Results

We identified three novel CpG sites (cg14671384, cg25540824, and cg10848724) significantly associated with BMI change. Two of the identified CpG sites were located in regions previously associated with body shape and basal metabolic rate. Annotation of the top 20 BMI change-associated CpGs revealed strong connections to obesity and T2D. Notably, these CpGs exhibited active regulatory roles and located in genes with high expression in the liver and digestive tract, suggesting a potential regulatory pathway from genome to phenotypes of energy metabolism and absorption via DNA methylation. Cross-sectional and longitudinal EWAS comparisons indicated different mechanisms between CpGs related to BMI and BMI change.

Conclusion

This study enhances our understanding of the epigenetic dynamics underlying BMI change and emphasizes the value of longitudinal analyses in deciphering the complex interplay between epigenetics and obesity.
Role of hypoxia-mediated pyroptosis in the development of extending knee joint contracture in rats
Authors: Zhang Quan-Bing , Huo Lei , Li Mian , Zhang Rui , Zhou Ting , Wang Feng , Zhou Yun
Published: May 2024

Joint contracture is one of the common diseases clinically, and joint capsule fibrosis is considered to be one of the most important pathological changes of joint contracture. However, the underlying mechanism of joint capsule fibrosis is still controversial. The present study aims to establish an animal model of knee extending joint contracture in rats, and to investigate the role of hypoxia-mediated pyroptosis in the progression of joint contracture using this animal model. 36 male SD rats were selected, 6 of which were not immobilized and were used as control group, while 30 rats were divided into I-1 group (immobilized for 1 week following 7 weeks of free movement), I-2 group (immobilized for 2 weeks following 6 weeks of free movement), I-4 group (immobilized for 4 weeks following 4 weeks of free movement), I-6 group (immobilized for 6 weeks following 2 weeks of free movement) and I-8 group (immobilized for 8 weeks) according to different immobilizing time. The progression of joint contracture was assessed by the measurement of knee joint range of motion, collagen deposition in joint capsule was examined with Masson staining, protein expression levels of HIF-1α, NLRP3, Caspase-1, GSDMD-N, TGF-β1, α-SMA and p-Smad3 in joint capsule were assessed using western blotting, and the morphological changes of fibroblasts were observed by transmission electron microscopy. The degree of total and arthrogenic contracture progressed from the first week and lasted until the first eight weeks after immobilization. The degree of total and arthrogenic contracture progressed rapidly in the first four weeks after immobilization and then progressed slowly. Masson staining indicated that collagen deposition in joint capsule gradually increased in the first 8 weeks following immobilization. Western blotting analysis showed that the protein levels of HIF-1α continued to increase during the first 8 weeks of immobilization, and the protein levels of pyroptosis-related proteins NLRP3, Caspase-1, GSDMD-N continued to increase in the first 4 weeks after immobilization and then decreased. The protein levels of fibrosis-related proteins TGF-β1, p-Smad3 and α-SMA continued to increase in the first 8 weeks after immobilization. Transmission electron microscopy showed that 4 weeks of immobilization induced cell membrane rupture and cell contents overflow, which further indicated the activation of pyroptosis. Knee extending joint contracture animal model can be established by external immobilization orthosis in rats, and the activation of hypoxia-mediated pyroptosis may play a stimulating role in the process of joint capsule fibrosis and joint contracture.
Craniomandibular transverse tomographic evaluation after anterior open bite orthodontic treatment with miniplates anchorage
Authors: de Mesquita Enio Vitor , Meloti Fernanda , Silva Ertty , de Almeida Cardoso Mauricio , An Tien-Li , do Carmo Chagas Nascimento Monikelly
Published: May 2024

Background

Skeletal anterior open bite (SAOB) represents one of the most complex and challenging malocclusions in orthodontics. Orthodontic treatment supported by miniplates enable to reduce the need for orthognathic surgery. Transverse dimension may be affected by intrusion biomechanics. This study aims to assess transverse bone alterations in patients with SAOB who underwent orthodontic treatment with absolute anchorage using four miniplates.

Methods

A total of 32 patients of both sexes, with an average age of 33.8 years, diagnosed with SAOB and treated orthodontically with four miniplates (one in each hemiarch), were selected for this study. Tomographic examinations were performed before (T1) and after (T2) orthodontic treatment. Linear measurements (width of the maxillary base, maxillary alveolar, maxillary root, maxillary dental cusp, mandibular alveolar) and angular measurements (maxillary intermolar angle) were assessed in these images. The Shapiro-Wilks normality tests were applied to verify data distribution, and the paired t-test was used to compare the initial and final measures obtained.

Results

Among the evaluated parameters, the maxillary alveolar width, maxillary dental cusp width, mandibular alveolar cusp width, and intermolar angle showed statistically significant differences between T1 and T2 (p < 0.05). However, maxillary base and maxillary root widths showed no significant difference (p > 0.05).

Conclusions

Intrusion and distalization with miniplates in SAOB therapy may lead to significant expansive changes, due to molars cusps width and buccal inclination increase restricted at the alveolar level.
A comparative analysis of particulate bovine bone substitutes for oral regeneration: a narrative review
Authors: Pabst Andreas , Becker Philipp , Götz Werner , Heimes Diana , Thiem Daniel G.E. , Blatt Sebastian , Kämmerer Peer W.
Published: May 2024

Purpose

Particulate bovine bone substitutes (BS) are commonly used in oral regeneration. However, more literature is needed focusing on comparative analyses among various particulate bovine BS. This study evaluates pre-clinical and clinical data of different particulate bovine BS in oral regeneration.

Methods

A narrative review was conducted by screening the PubMed database Included in the review were pre-clinical and clinical studies until 2024 comparing a minimum of two distinct particulate bovine BS. In addition to examining general data concerning manufacturing and treatment processes, biological safety, physical and chemical characteristics, and graft resorption, particular emphasis was placed on assessing pre-clinical and clinical data related to ridge preservation, sinus floor elevation, peri-implant defects, and various forms of alveolar ridge augmentation utilizing particulate bovine BS.

Results

Various treatment temperatures ranging from 300 to 1,250 °C and the employment of chemical cleaning steps were identified for the manufacturing process of particulate bovine BS deemed to possess biosecurity. A notable heterogeneity was observed in the physical and chemical characteristics of particulate bovine BS, with minimal or negligible graft resorption. Variations were evident in particle and pore sizes and the porosity of particulate bovine BS. Pre-clinical assessments noted a marginal inclination towards favorable outcomes for particulate bovine BS subjected to higher treatment temperatures. However, clinical data are insufficient. No distinctions were observed regarding ridge preservation, while slight advantages were noted for high-temperature treated particulate bovine BS in sinus floor elevation.

Conclusions

Subtle variances in both pre-clinical and clinical outcomes were observed in across various particulate bovine BS. Due to inadequate data, numerous considerations related to diverse particulate bovine BS, including peri-implant defects, must be more conclusive. Additional clinical studies are imperative to address these knowledge gaps effectively.

Graphical abstract
Spatiotemporal multi-omics: exploring molecular landscapes in aging and regenerative medicine
Authors: Chu Liu-Xi , Wang Wen-Jia , Gu Xin-Pei , Wu Ping , Gao Chen , Zhang Quan , Wu Jia , Jiang Da-Wei , Huang Jun-Qing , Ying Xin-Wang , Shen Jia-Men , Jiang Yi , Luo Li-Hua , Xu Jun-Peng , Ying Yi-Bo , Chen Hao-Man , Fang Ao , Feng Zun-Yong , An Shu-Hong , Li Xiao-Kun , Wang Zhou-Guang
Published: May 2024

Aging and regeneration represent complex biological phenomena that have long captivated the scientific community. To fully comprehend these processes, it is essential to investigate molecular dynamics through a lens that encompasses both spatial and temporal dimensions. Conventional omics methodologies, such as genomics and transcriptomics, have been instrumental in identifying critical molecular facets of aging and regeneration. However, these methods are somewhat limited, constrained by their spatial resolution and their lack of capacity to dynamically represent tissue alterations. The advent of emerging spatiotemporal multi-omics approaches, encompassing transcriptomics, proteomics, metabolomics, and epigenomics, furnishes comprehensive insights into these intricate molecular dynamics. These sophisticated techniques facilitate accurate delineation of molecular patterns across an array of cells, tissues, and organs, thereby offering an in-depth understanding of the fundamental mechanisms at play. This review meticulously examines the significance of spatiotemporal multi-omics in the realms of aging and regeneration research. It underscores how these methodologies augment our comprehension of molecular dynamics, cellular interactions, and signaling pathways. Initially, the review delineates the foundational principles underpinning these methods, followed by an evaluation of their recent applications within the field. The review ultimately concludes by addressing the prevailing challenges and projecting future advancements in the field. Indubitably, spatiotemporal multi-omics are instrumental in deciphering the complexities inherent in aging and regeneration, thus charting a course toward potential therapeutic innovations.
Surgical treatment for pulmonary metastasis from ovarian cancer: a retrospective case series
Authors: Tsubouchi Saki , Tsukamoto Yo , Ishikawa Ai , Shigemori Rintaro , Kato Daiki , Shibazaki Takamasa , Mori Shohei , Nakada Takeo , Odaka Makoto , Ohtsuka Takashi
Published: May 2024

Background

Distant metastases of ovarian cancer are rarely detected alone. The effectiveness of surgical intervention for pulmonary metastases from ovarian cancer remains uncertain. This study aimed to investigate the clinicopathologic characteristics and outcomes of patients undergoing resection for pulmonary metastasis from ovarian cancer.

Case presentation

The clinicopathologic characteristics and outcomes of radical surgery for pulmonary metastasis from ovarian cancer were investigated. Out of 537 patients who underwent pulmonary metastasis resection at two affiliated hospitals between 2010 and 2021, four (0.74%) patients who underwent radical surgery for pulmonary metastasis from ovarian cancer were included. The patients were aged 67, 47, 21, and 59 years; the intervals from primary surgery to detection of pulmonary metastasis from ovarian cancer were 94, 21, 36, and 50 months; and the overall survival times after pulmonary metastasectomy were 53, 50, 94, and 34 months, respectively. Three of the four patients experienced recurrence after pulmonary metastasectomy. Further, preoperative carbohydrate antigen (CA) 125 levels were normal in two surviving patients and elevated in the two deceased patients.

Conclusion

In this study, three of the four patients experienced recurrence after pulmonary metastasectomy, but all patients survived for > 30 months after surgery. Patients with ovarian cancer and elevated CA125 levels may not be optimal candidates for pulmonary metastasectomy. To establish appropriate criteria for pulmonary metastasectomy in patients with ovarian cancer, further research on a larger patient cohort is warranted.
Dominance structure and constancy of spiders in the Indian Thar desert
Authors: Kashmeera Neisseril Anirudhan , Sudhikumar Ambalaparambil Vasu
Published: May 2024

Background

The knowledge about the species of a habitat (both resident and transient/dominant and rare) is a vital step to plan the conservation measures. Being generalist predators, spiders help controlling the population of their prey and maintain ecosystem stability. This makes spiders excellent bio-indicators for assessing the impact of anthropogenic disturbance factors on natural ecosystems (De, Siliwal, Uniyal and Hussain in Trop. Ecol. 63: 1–7, 2021). The aim of this study was to assess the dominance structure and constancy of spiders in three different habitats (Sand dunes, Riparian and Rocky) of the Thar desert. Study was conducted from March 2017 to February 2019 covering all seasons.

Results

The eudominant species in sand dunes and rocky desert belonged to the family Thomisidae. These species were Tmarus sp. 1 and Tmarus kotigeharus Tikader, 1963, respectively. There were two eudominants in Riparian habitat (Oecobius putus O. Pickard-Cambridge, 1876 and Menemerus bivittatus (Dufour, 1831)). Through the analysis of constancy of all the species in the Thar desert, it was revealed that three species were constant in all the three habitats. The number of accessory and accidental species was far higher than constant species in all the habitats.

Conclusions

The number of accessory and accidental spider species in all habitats was far higher than constants due to the unstability of spider population. Therefore, this study highlights the necessity for conservation of these habitats of the Thar desert.
Investigating the bacterial consortia properties of electrogenic anodic biofilm in a double-chamber microbial fuel cell: electrochemical, physical, biochemical and molecular characterization
Authors: Zater Doaa Khodary , Elzamik Fatma I. , Abdel Basit Howaida M. , Moustafa Gamal El-Din M. , Khater Dena Z. , El-Khatib Kamel M.
Published: May 2024

This work evaluated the electrochemical, physical, biochemical, and molecular characterization of electrogens from a graphite felt anode when zinc oxide on activated carbon (ZnO/AC) was used as a cathodic electrocatalyst in a double-chambered microbial fuel cell (DCMFC). The electrochemical polarization behavior of the DCMFC showed that ZnO/AC had a higher power density (PDmax) of 89 mW m−2 with a corresponding cell current density (CD) of 248 mA m−2 and a voltage output of 395 mV, which was higher than those of the blank electrode used as a benchmark (PDmax of 68 mW m−2 at a CD of 161 mA m−2 and a voltage of 421 mV). Furthermore, scanning electron microscopy and transmission electron microscopy revealed that the morphology and interior properties of the strains varied among the rods (bacilli), spirals (vibrios), and spheres (diplococci, staphylococci and streptococci). In addition, biochemical characterization via the Vitek2 compact system and molecular analysis via 16 S rRNA and 18 S rRNA gene sequencing revealed the occurrence of nine prevalent species that were correlated with Sphingobacterium spiritivorum, Ochrobactrum anthropicus, Pseudomonas mendocina, Stenotrophomonas maltophilia, Leuconostoc mesenteroides, Staphylococcus equorum, Bacillus subtilis HQ334981.1, Kocuria kristinae KC581674.1 and Saccharomyces cerevisiae NR111007.1. Consequently, the present study outlines different characterization strategies for electrogenic microbes that play an important role in the overall performance of DCMFC for scaling up and managing existing environmental pollution for sustainable energy generation.
Prognosis of transarterial chemoembolization-sorafenib compared to transarterial chemoembolization-alone in hepatocellular carcinoma stage C: a systematic review
Authors: Mulyadi Rahmad , Hasan Irsan , Sidipratomo Prijo , Putri Pungky Permata
Published: May 2024

Background

This systematic review aims to compare the prognosis of treatment transarterial chemoembolization (TACE) combined with sorafenib and TACE-alone in patients with hepatocellular carcinoma (HCC) with Barcelona clinic liver cancer-stage C (BCLC-C).

Materials and methods

A systematic search was conducted on five electronic databases: PubMed, ScienceDirect, Cochrane, Embase, and Scopus. Studies were included if they compared overall survival (OS) of TACE-Sorafenib to TACE-alone in patients with HCC BCLC-C within the 2019–2023 timeframe. We excluded studies consisting of conference abstracts, letters, editorials, guidelines, case reports, animal studies, trial registries, and unpublished work. The selected articles were evaluated from August 2023 to September 2023. The journal’s quality was assessed with NOS for a non-randomized controlled trial.

Results

This systematic review included four studies following the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA). All four studies compared the OS of 401 patients with TACE-sorafenib to TACE-alone. Two studies compared time-to-progression (TTP), one study compared progression-free survival (PFS), and two studies compared disease control rate (DCR). There were various population criteria, TACE techniques used, risk factors, follow-up time, and adverse events. The collected evidence generally suggested that the combination of TACE-sorafenib is superior compared to TACE-alone. Due to a lack of essential data for the included study, a meta-analysis couldn't be performed.

Conclusion

The results of this systematic review suggested that TACE-sorafenib combination therapy in patients with HCC BCLC-C improves OS superior compared to TACE-alone, without a notable increase in adverse events.
Comparative study between ultrasound and MR enterography in evaluation of Crohn’s disease
Authors: Mahdy Nada Sayed , El-Gaafary Sahar Mohammed , Mageed Khaled Hamdy Abdel , Shehata Khaled A. Ali , AbdelKarim Maha Ahmed Sayed , Abdulhafiz Essam Mohamed
Published: May 2024

Background

Crohn’s disease is a chronic inflammatory bowel disease that can affect any part of the gut. Endoscopy is the gold standard for diagnosis, but it only assesses mucosal lesions. Magnetic resonance enterography (MRE) can assess disease presence and activity, but it has limitations such as motion sensitivity, long scan time, and high cost. Bowel sonography has been introduced as a non-invasive, practical, safe, and low-cost technique to assess disease activity and complications. In our study we aim to assess the comparability of ultrasound to MRE in evaluation of patients with Crohn’s disease, and its complications.

Results

Twenty-five patients with 38 Crohn’s disease affected segments were evaluated by bowel ultrasound (BUS) and MRI enterography (MRE), where BUS and MRE showed equivalent diagnostic performance for disease detection and localization (97.4%, 100%), for sensitivity and specificity of both modalities. Peri-mural fluid (89.5%, 94.4%) and mural stratification loss (100%, 100%) showed high sensitivity and specificity by BUS compared to MRE, while for assessment of mural vascularity, BUS showed high sensitivity and specificity for high grade vascularity (100%, 83.3%), but low sensitivity and high specificity for low and moderate vascularity (0%–62.5%, 81.8%) compared to MRE. Complications including fistulae and abscessed were all correctly identified in BUS compared to MRE.

Conclusion

BUS showed comparable results to MRE for identification, localization, assessment of findings related to disease activity, and complications in cases of Crohn’s disease rendering it a viable alternative to MRE.
Medical Aspects of Crime by Sir William Norwood East, 1936
Authors: Gunn John
Published: May 2024

This is areview of an early and important textbook published by Sir William Norwood East.
Investigating the association of opioid prescription with the incidence of psychiatric disorders: nationwide cohort study in South Korea
Authors: Oh Tak Kyu , Park Hye Yoon , Song In-Ae
Published: May 2024

Background

The relationship between opioid use and the incidence of psychiatric disorders remains unidentified.

Aims

This study examined the association between the incidence of psychiatric disorders and opioid use.

Method

Data for this population-based cohort study were obtained from the National Health Insurance Service of South Korea. The study included all adult patients who received opioids in 2016. The control group comprised individuals who did not receive opioids in 2016, and were selected using a 1:1 stratified random sampling procedure. Patients with a history of psychiatric disorders diagnosed in 2016 were excluded. The primary end-point was the diagnosis of psychiatric disorders, evaluated from 1 January 2017 to 31 December 2021. Psychiatric disorders included schizophrenia, mood disorders, anxiety and others.

Results

The analysis included 3 505 982 participants. Opioids were prescribed to 1 455 829 (41.5%) of these participants in 2016. Specifically, 1 187 453 (33.9%) individuals received opioids for 1–89 days, whereas 268 376 (7.7%) received opioids for ≥90 days. In the multivariable Cox regression model, those who received opioids had a 13% higher incidence of psychiatric disorder than those who did not (hazard ratio 1.13; 95% CI 1.13–1.14). Furthermore, both those prescribed opioids for 1–89 days and for ≥90 days had 13% (hazard ratio 1.13, 95% CI 1.12–1.14) and 17% (hazard ratio 1.17, 95% CI 1.16–1.18) higher incidences of psychiatric disorders, respectively, compared with those who did not receive opioids.

Conclusions

This study revealed that increased psychiatric disorders were associated with opioid medication use. The association was significant among both short- and long-term opioid use.
Επικοινωνία και ψηφιακός κόσμος στο πολιτιστικό απόθεμα της Αθωνικής Πολιτείας
Authors: Πατσέας Κωνσταντίνος
Published: May 2024

Η παρούσα διδακτορική Διατριβή μελετά τις άυλες και υλικές παραμέτρους αξιοποίησης των τεχνολογιών πληροφορικής και επικοινωνιών (ΤΠΕ) για τη διαχείριση της χριστιανικής πολιτιστικής κληρονομιάς και δη αυτής του Αγίου Όρους, στο πλαίσιο του ψηφιακού μετασχηματισμού των σύγχρονωνκοινωνιών. Κύρια στοχοθεσία της έρευνας υπήρξε η καταγραφή και ανάλυση όλων των παραμέτρων θεολογικών, πολιτιστικών, τεχνολογικών που μπορούν να διαμορφώσουν καλές πρακτικές στην διαχείριση θρησκευτικών πολιτιστικών θησαυρών, αντίστοιχων φορέων, χωρίς την αλλοίωση του πνευματικούτους πυρήνα. Γίνεται ακριβής διατύπωση των τελικών συμπερασμάτων της έρευνας και καταγράφονται οι προτάσεις που αφορούν στην περαιτέρω αξιοποίηση της προσπάθειας αξιοποίησης των σύγχρονων τεχνολογιών πληροφορικής και επικοινωνιών από θρησκευτικούς φορείς για την διάσωση, προβολή τουπολιτιστικού τους αποθέματος.
Exploring the Feasibility of Solar and Wind Energy Utilization in Poland: A Hybrid Energy Mapping Approach
Authors: Denis Agata , Xydis George
Published: May 2024

Poland is obliged to reach the target of 21% share of renewable energy sources in gross final energy consumption by 2030. Thus, it makes it necessary and obligatory for the country to expand its wind and solar power generation. This research presents the preliminary process of creating the hybrid energy map for the country that would make it simpler both for the government and the enterprises to choose the available siting location. Geodata is assessed along with the siting constraints in a spatial analysis by using Geographic Information System (GIS). The final outcome of the paper is the visual hybrid energy map of Poland showing the available space for both wind and solar farms.
PREDICTION OF THE PRESENCE OF CHLAMYDIA INFECTION IN PERSISTENT YEAR-ROUND ALLERGIC RHINITIS
Authors: Portenko Elena G. , Burdo Georgy B. , Kuznetsova Valeria S. , Vashnevskaya Natalia A.
Published: May 2024

Background. Taking into account a number of features in the diagnosis of year-round allergic rhinitis caused by chlamydial infection, it is important to increase the efficiency of its detection.
Purpose. Creation of a methodology for predicting the presence of chlamydial infection in perennial allergic rhinitis, suitable for subsequent implementation in the form of a database of an expert decision support system.
Methods. Based on the literature analysis of the research results, the results of managing patients with confirmed year-round allergic rhinitis, who, according to the results of basic treatment, have an unstable remission, the symptoms of this disease were established and a method for predicting its presence was formulated. All symptomatic data were divided into four groups of signs with a certain number of indicators in each. A total of 23 indicators have been proposed, some of which are mandatory for confirming infection, and some are alternative. The forecasting technique is based on the analysis of the ratio of mandatory and alternative features and was presented in the form of clear rules suitable for implementation in the MATLAB computing environment. The use of data related to patients was carried out in accordance with the Declaration of Helsinki of the World Medical Association, which regulates the conduct of scientific research.
Research results and discussion. Based on the complex of studies performed, a method for predicting the presence of this pathology was proposed. A feature of the technique is a gradual increase in the reliability of the forecast, which eliminates the redundancy of surveys. Taking into account the possibilities of modern information technologies, the problem of creating a knowledge base for expert systems for the diagnosis of year-round allergic rhinitis caused by chlamydial infection was solved.
The practical significance of the results of the work is to increase the reliability of diagnosing year-round allergic rhinitis caused by chlamydial infection.
Conclusion. It was found that the identified 23 diagnostic indicators provide a fairly reliable prediction of the presence of chlamydial infection and, as a result, improve the quality of diagnosis of year-round allergic rhinitis.
SENSITIVITY BASELINE AND ORCHARD POPULATIONS OF VENTURIA INAEQUALIS TO THE SUCCINATE DEHYDROGENASE INHIBITOR FUNGICIDES (SDHI)
Authors: Nasonov Andrei I. , Yakuba Galina V. , Astapchuk Irina L. , Marchenko Nikita A.
Published: May 2024

Background. Scab plays the leading role in terms of significance and harmfulness among apple-spotted spots. The use of fungicides with a highly specific mechanism of action against the pathogen can lead to the development of resistance in the pathogen.
Purpose. To assess the sensitivity of monospore isolates of Venturia inaequalis from a population with different histories of interaction with fungicides to the active substances of the SDHI chemical class boscalid and fluxapiroxad in vitro.
Materials and methods. The in vitro sensitivity of isolates was assessed by mycelial growth at various concentrations of boscalid and fluxapiroxad and expressed as an effective 50% concentration (EC50).
Results. Boscalid EC50 values for all populations ranged from 0.04 to <300 mg a.i./l, and the average value was 16.27 mg a.i./l. The resistance factor (RF) for boscalid in various garden populations varied from 2 to 8, and the proportion of resistant isolates was not high. The population mean EC50 of fluxapiroxade was 0.259 mg a.i./l. The RF for fluxapiroxad did not exceed 4, and for two garden populations it was only 2, which indicates a slight shift in sensitivity in these populations relative to the "initial".
Conclusion. For the first time in the world, the EC50 value of the boscalid was obtained for the initial population. The activity of fluxapiroxad against the pathogen was higher than that of boscalid. For all orchards populations, a decrease in sensitivity to the investigated fungicides was shown in comparison with the original population. It is assumed that a relatively low shift in sensitivity to carboxamides for the studied populations is due to the use of these DIs in the region in the form of mixed preparations with fungicides that have a different mechanism of action.
SPATIAL ASPECTS OF EMPLOYMENT DEVELOPMENT IN NORTHERN ASIA
Authors: Ivanova Sembrika N.
Published: May 2024

Background. The article deals with open aspects of the development of employment in North Asia. Of particular importance is the location of labor resources. Solving the problem of the totality of socio-economic development between regions is a basic condition for ensuring national security. Northern Asia is an appraisal area, since it contains the main natural resource potentials of our country, which account for a quarter of the total population and produce almost a third of GRP.
Purpose. To study the features of the spatial development of employment of the population and the impact of national trends, industry specialization, sub-regional specifics and intra-regional regulation on the labor market of the territories of North Asia.
Materials and methods. Methods of comparative analysis and economic-statistical methods are used. A comprehensive assessment of the spatial aspects of the development of employment in the regions of North Asia was carried out on the basis of an adapted method of structural shifts. The information base was the data of the Federal State Statistics Service.
Results. The causes and contribution of individual changes in the structure of employment of the population of a particular region are identified. Based on the calculation of the localization coefficient, the territories of North Asia were identified, in which agriculture in the period under review is: the basic type of economic activity; local (regional significance) or is outside the specialization of the region.
Conclusion. The method of structural shifts makes it possible to formulate in-depth conclusions about the transformations in the regional labor markets. Studies have shown that in order to increase the attractiveness of North Asia in terms of investment and reduce migration risks, especially in rural areas, it is necessary to take measures aimed at increasing the availability of infrastructure services, including social, transport, medical, and educational services. The main conclusions and recommendations can be used by the legislative and executive authorities in the development of programs to promote employment and socio-economic development of the regions of Asian Russia.
Funding. The study was carried out within the framework of the state task of the Baikal Institute of Nature Management SB RAS Project No. АААА-А21-121011590039-6 (0273-2021-0003).
STRUCTURAL FEATURES AND ORIGIN OF CRESCENT CELLS OF SOME BLABERIDAE
Authors: Grebtsova Elena A. , Prisnyi Andrey A.
Published: May 2024

The purpose of the research is search for species of the family Blaberidae, which hemolymph includes crescent cells. To study the structural features of these hemocytes and their origin.
Materials and methods. The hemolymph of nymphs and imagos of Blaberus craniifer, Blaberus marajoara, Blaptica dubia, Gromphadorhina portentosa, Perisphaerus serville, Archimandrita tesselata, Pycnoscelus indicus, Gyna lurida, Pseudoglomeris magnifica, Simandoa conserfariam were studied. Using light microscopy we determined the quantitative content of crescent cells and their morphological features. Glycosaminglycans were detected by staining with alcyan blue (pH 1.0). Hemocytes were measured using NIS-Elements software. Due to the AFM scanning it possible to study the topography of the cell surface. Cell scanning, analysis and processing of AFM data in Nova and Image Analysis P9 applications.
Results. Among 10 species of the family Blaberidae, only three have sickle-shaped cells in the hemolymph: Gromphadorhina portentosa, Blaptica dubia, Archimandrita tesselata. They all belong to the same subfamily Blaberidae. The study of insects of different ages allows to identify intermediate forms and trace the stages of development of crescent cells and their relationship with spherulocytes. Scanning of cells gave additional information about size of hemocytes and their surface area. The change in the roughness parameters of spherulocytes, intermediate cells of nymph up to the characteristic crescent shape was determined.
Sensory Processing Sensitivity as a trait of temperament - evolutionary, socio-cultural, biological context and relation to mental disorders
Authors: Kurczewska Ewa , Ferensztajn-Rochowiak Ewa , Rybakowski Janusz , Rybakowski Filip
Published: May 2024

W artykule przedstawiono cechę Wrażliwości Przetwarzania Sensorycznego (Sensory Processing Sensitivity, SPS), jej charakterystykę, narzędzie oceny oraz związek z zaburzeniami psychicznymi na podstawie analizy piśmiennictwa dotyczącego SPS od 1997 roku.
Przedstawiono przegląd badań dotyczących SPS w kilku istotnych kontekstach: ewolucyjnym/adaptacyjnym, społeczno-kulturowym, temperamentalnym/osobowościowy oraz biologicznym, uwzględniając wpływ czynników genetycznych oraz aktywności poszczególnych obszarów ośrodkowego układu nerwowego, związanych z przetwarzaniem bodźców emocjonalnych i poznawczych. Wysoka Wrażliwość Przetwarzania Sensorycznego jest cechą wrodzoną, uwarunkowaną biologicznie i modulującą procesy rozwojowe, występującą u 20-35% populacji ogólnej niezależnie od płci. Charakteryzuje się głębszym przetwarzaniem bodźców, łatwością przestymulowania, silnymi reakcjami emocjonalnymi i więziami empatycznymi oraz wyczuleniem na subtelności w otaczającym świecie. Nie jest tożsama z introwersją, ani neurotyzmem, choć wykazuje do nich pewne podobieństwa. SPS może wiązać się z podatnością do rozwoju szerokiego spektrum objawów i zaburzeń psychicznych, m.in. zaburzeń depresyjnych i lękowych, fobii społecznej, aleksytymii, wypalenia zawodowego oraz zaburzeń internalizacyjnych i eksternalizacyjnych oraz mutyzmu wybiórczego u dzieci.
Editorial 2/2024
Authors: Dudek Dominika , Sobański Jerzy A. , Klasa Katarzyna
Published: May 2024

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Managing Multiple Information Sources for a Questioning Agenda
Authors: Łupkowski Paweł , Urbański Mariusz
Published: May 2024

In this paper, we consider how an agent may manage a questioning agenda in a situation where multiple information sources are available. We work within the framework of formal dialogue systems with the underpinning of Inferential Erotetic Logic. Firstly, we present the formal dialogue system DL(IEL)mult for managing multi-agent information retrieval. Then, we extend the proposed system so that it is capable of representing group and individual levels for the question decomposition process. We also propose two measures for evaluating information sources: their cooperativeness and success levels; next, we analyse how the choice of agents may influence the way in which a solution for a given problem is reached.
La cara de la paz: gobierno, pedagogía de paz y desinformación en Colombia
Authors: Burnyeat Gwen
Published: May 2024

El acuerdo de paz colombiano con la guerrilla de las FARC buscó poner fin a cincuenta años de guerra y le valió al presidente Juan Manuel Santos el Premio Nobel de Paz. Sin embargo, la sociedad colombiana rechazó el acuerdo en un plebiscito polarizante, celebrado en medio de una emotiva campaña de desinformación. Gwen Burnyeat se unió a la Oficina del Alto Comisionado para la Paz, la institución gubernamental a cargo de las negociaciones de paz, para observar y participar en una estrategia innovadora de “pedagogía de paz” que tenía el propósito de explicar el acuerdo a la sociedad colombiana. Esta etnografía multiescalar revela los desafíos que los oficiales del gobierno experimentaron al comunicarse con audiencias escépticas y al intentar traducir el contenido del acuerdo a la opinión pública. Burnyeat plantea que el proceso de paz se vio perjudicado sobre todo por las relaciones gobierno-sociedad, inmersas en lógicas culturalmente liberales y moldeadas por las políticas de donantes internacionales. La cara de la paz muestra el caso colombiano como un espejo en el que aparece reflejada la crisis del liberalismo del Norte global y en el que se desvanece la ilusión de racionalidad que tanto desvela al liberalismo y condiciona sus reacciones a la ‘posverdad’
EQUIPE MULTIPROFISSIONAL
Authors: Monteiro Priscila Carvalho , De Melo Marilândes Mol Ribeiro
Published: May 2024

No contexto educacional, as ações desenvolvidas multiprofissionalmente são reconhecidas como pedagógicas tanto por aqueles que as desenvolvem, quanto pela comunidade escolar. O presente artigo demonstra os dados obtidos através de pesquisa qualitativa baseada em Silva (2005) e Minayo (1999), com o objetivo de compreender sobre a atuação e percepções em relação ao desenvolvimento do trabalho multiprofissional. O referido estudo foi de campo, a partir de Lakatos e Marconi (2001), e teve como método de coleta de dados a utilização de questionário semiestruturado. A aplicação do questionário ocorreu no ano de 2022, 6 (seis) profissionais participaram da pesquisa e descreveram a respeito de sua atuação. A análise dos dados coletados foi conduzida a partir dos critérios estabelecidos nos estudos de Bourdieu (1930-2002), Dubar (1945-2015), entre outros, considerando as atividades desenvolvidas pela equipe multiprofissional do campus Araquari. Os resultados elencados demonstram o perfil profissional da equipe multiprofissional, o envolvimento da equipe no cotidiano profissional, além da proatividade e parceria, destaca-se a necessidade do rompimento com questões limitantes oriundas da própria profissão, pois no desenvolvimento de funções, no campo educacional, os profissionais possuem suas representações orientadas por seus movimentos neste campo. Além disso, reafirma-se a importância do referido trabalho no campo educacional, destaca-se a importância da criação de uma política na própria rede de Educação Profissional e Tecnológica para o fortalecimento das ações desenvolvidas pelos/as profissionais. Ademais, elenca-se a necessidade de criação de uma política na rede de educação básica com a previsão de profissionais que possam atuar em equipe multiprofissional, visando a qualificação para a formação humana.
FORMAÇÃO HUMANA INTEGRAL
Authors: Magalhães Nadja Regina Sousa , Martini Lucas , Brandt Andressa Graziele
Published: May 2024

Considerando a importância e relevância da Educação Integral em um âmbito teórico e prático para a formação de alunos críticos e curiosos em perspectiva da formação integral, o presente artigo emerge a partir da análise de questionários preenchidos por quatorze docentes participantes de um minicurso desenvolvido no III Seminário Mineiro de Educação Integral (SEMEI) pelas pessoas autoras do texto. O objetivo geral consiste na produção de sentidos compreendidos nos discursos de pessoas docentes frente a participação em um minicurso de Educação Integral. Para atingir o objetivo, utilizamos a Análise de Discurso (AD) Franco-brasileira no viés de Eni P. Orlandi (2012, 2017) como fundamentação teórica e metodológica para a produção dos sentidos que movimentam esta pesquisa. Em relação à Educação Integral, as discussões dialogam com autores como Cavaliere (2009) e Parente (2010). Os sentidos emergentes apontam para os diversos desafios enfrentados pela realidade docentes para a concretização da perspectiva de Educação Integral.
NO CONTEXTO DE CIBERCULTURA, O QUE PODE A FOTOGRAFIA NO ENSINO DE GEOGRAFIA?
Authors: Fernandes Nathan Moretto Guzzo , Borges Vilmar José , Martins Rebeca Soterio , Sousa Shirlliney Virginio de , Tiago Cardoso Melo
Published: May 2024

O presente artigo objetiva pensar a fotografia como alternativa aos métodos convencionais no ensino de geografia, considerando-a uma linguagem criadora de conhecimentos e produtora de saberes que permite aos estudantes serem participantes do processo de criação, produção e pensamento sobre o espaço geográfico em que estão inseridos. Para tal finalidade, dialoga com autores que trabalham conceitos fundamentais para a disciplina de geografia bem como com autores que estudam fotografia e sua contribuição à educação. Utiliza, como aportes metodológicos, o levantamento bibliográfico e o uso de oficinas pedagógicas como ferramenta de produção coletiva e de registro de informações. O trabalho justifica-se pela ampliação do uso de artefatos tecnológicos em geral e, em particular, da fotografia na educação no contexto de cibercultura. Aposta no cotidiano escolar como lugar privilegiado para criações curriculares. E, assim, deriva deste trabalho a realização de uma oficina de fotografias com estudantes do 9º ano de uma escola pública de ensino fundamental. Como reverberações da oficina, os estudantes puderam repensar seus cotidianos e espaços de vivências dentro de múltiplos e contínuos mundos a partir das imagens fotográficas registradas por eles e ainda compreender conceitos geográficos. Conclui que diferentes linguagens, como a fotografia, têm grande potencial pedagógico no ensino de geografia.
Polar Fungi Pseudogymnoascus: Secondary Metabolites and Ecological Significance
Authors:   Institute of Ocean and Earth Sciences, Universiti Malaya, 50603 Kuala Lumpur, Malaysia, Satriawan Herland , Teoh Teow Chong ,   Institute of Ocean and Earth Sciences, Universiti Malaya, 50603 Kuala Lumpur, Malaysia,   Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia, Rizman-Idid Mohammed ,   Institute of Ocean and Earth Sciences, Universiti Malaya, 50603 Kuala Lumpur, Malaysia, Krishnan Abiramy ,   National Antarctic Research Center, Universiti Malaya, 50603 Kuala Lumpur, Malaysia,   MAHSA University, Jln SP 2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Bakar Nurlizah Abu ,   National Antarctic Research Center, Universiti Malaya, 50603 Kuala Lumpur, Malaysia, Alias Siti Aisyah ,   Institute of Ocean and Earth Sciences, Universiti Malaya, 50603 Kuala Lumpur, Malaysia,   National Antarctic Research Center, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
Published: May 2024

Fun gi are widely distributed organisms known to produce secondary metabolites and other biochemical compounds. However, fungi from the polar regions with extreme environmental conditions such as low temperatures, high ultra-violet exposure, nutrient scarcity, and dryness are understudied. They produced many secondary metabolites that are yet to be identified, to adapt and survive the extreme conditions. These polar fungi with potential in secondary metabolites are promising in biotechnological applications such as pharmaceutical, textile, and food manufacturing. Pseudogymnoascus is a genus within the Pseudeurotiaceae family that has been reported in the Antarctic regions. This genus plays an important role in the decomposition process in the polar regions. To date, there are 24 species identified within the genus Pseudogymnoascus in the world. The true potential of Pseudogymnoascus, especially those from the polar region, is yet to be explored. This review gives an overview of the polar Pseudogymnoascus spp. Based on their secondary metabolites production and understanding their ecological perspective in the extreme environment.
Ecological Characteristics of Culturable Myxobacteria in the Rhizosphere Soil of Saline-Tolerant Plants in the Ebinur Saline Wetlands
Authors:   School of Life Sciences, Shihezi University, Shihezi, 832000, P. R. China, He Bo , Zhong Zhentao ,   School of Life Sciences, Shihezi University, Shihezi, 832000, P. R. China, Hu Wenge ,   School of Life Sciences, Shihezi University, Shihezi, 832000, P. R. China, Chen Xuemei ,   School of Life Sciences, Shihezi University, Shihezi, 832000, P. R. China, Ding Cheng ,   School of Life Sciences, Shihezi University, Shihezi, 832000, P. R. China, Qi Xiaoyun ,   School of Life Sciences, Shihezi University, Shihezi, 832000, P. R. China
Published: May 2024

T his study investigated the ecological distribution of myxobacteria in the saline environment of the Ebinur Lake Wetland, in China, which provides a unique microecological environment for their survival. Culturable myxobacteria were isolated using traditional methods including Escherichia coli DH5α, rabbit feces, and filter paper flakes as food sources. Molecular identification along with physiological, biochemical assays and morphological characterization was carried out for the classification and identification of myxobacteria. Associations between soil environmental factors and myxobacterial diversity were analyzed using redundancy analysis. This study involved collecting rhizosphere soil from 15 species of salt-tolerant plants, ultimately yielding 15 strains of myxobacteria from eight of these species. Fifteen strains of myxobacteria were taxonomically placed by analyzing 16S rRNA homology, seven strains were closely related to Myxococcus virescens, six strains were closely related to Myxococcus fulvus and two strains were closely related to Myxococcus xanthus. Myxococcus virescens, the dominant cultivable species of myxobacteria in the Ebinur Lake Wetland, was isolated from various sampling sites using traditional methods. Analysis of antibiotic resistance revealed that myxobacterial strains M2, M6, M9, M13, and M15 are resistant to gentamicin. Furthermore, predation assays demonstrated that myxobacterial strain M14 exhibits the broadest spectrum of predation against both bacterial and fungal prey. The limited culturable myxobacterial diversity observed in the Ebinur Lake Wetland could potentially result from variations in soil physicochemical properties, vegetation type, and employed isolation methods. These findings enhance our comprehension of the myxobacterial resources within this specific salt desert ecosystem.
Doing (in)equality in Swedish families: women’s narratives of outsourcing domestic work
Authors: Silfver Ann-Louise , Widding Ulrika
Published: May 2024

Outsourcing domestic work is an established global phenomenon increasingly common in Sweden, especially since introducing the RUT reform offering tax deductions for domestic services. Little is known about Swedish families using domestic services. This article investigates the narratives of 12 Swedish women living in families using domestic services and what this means for their everyday family life. The results show that outsourcing in part is regarded as a solution to a gender equality problem as it relieves women from unpaid household work. However, the women’s narratives also reveal that even when domestic work is outsourced, the women continue to have the main responsibility for everyday family life. The article thus contributes insights into how gender equality in everyday family practices is negotiated when domestic work is outsourced.