bluuebunny/crossref_metadata_2025 huggingface dataset:
Scoping Review on Epigenetic Mechanisms in Primary Immune Thrombocytopenia
Authors: Tan Jian Hong , Ahmad Azahari Ahmad Hazim Syakir , Ali Adli , Ismail Noor Akmal Shareela
Published: February 2023

Immune Thrombocytopenia (ITP) is an autoimmune blood disorder that involves multiple pathways responsible for the homeostasis of the immune system. Numerous pieces of literature have proposed the potential of immune-related genes as diagnostic and prognostic biomarkers, which mostly implicate the role of B cells and T cells in the pathogenesis of ITP. However, a more in-depth understanding is required of how these immune-related genes are regulated. Thus, this scoping review aims to collate evidence and further elucidate each possible epigenetics mechanism in the regulation of immunological pathways pertinent to the pathogenesis of ITP. This encompasses DNA methylation, histone modification, and non-coding RNA. A total of 41 studies were scrutinized to further clarify how each of the epigenetics mechanisms is related to the pathogenesis of ITP. Identifying epigenetics mechanisms will provide a new paradigm that may assist in the diagnosis and treatment of immune thrombocytopenia.
LhANS-rr1, LhDFR, and LhMYB114 Regulate Anthocyanin Biosynthesis in Flower Buds of Lilium ‘Siberia’
Authors: Fang Shaozhong , Lin Mi , Ali Muhammad Moaaz , Zheng Yiping , Yi Xiaoyan , Wang Shaojuan , Chen Faxing , Lin Zhimin
Published: February 2023

The bulb formation of Lilium is affected by many physiological and biochemical phenomena, including flower bud differentiation, starch and sucrose accumulation, photoperiod, carbon fixation, plant hormone transduction, etc. The transcriptome analysis of flower buds of Lilium hybrid ‘Siberia’ at different maturity stages showed that floral bud formation is associated with the accumulation of anthocyanins. The results of HPLC-MS showed that cyanidin is the major anthocyanin found in Lilium ‘Siberia’. Transcriptome KEGG enrichment analysis and qRT-PCR validation showed that two genes related to flavonoid biosynthesis (LhANS-rr1 and LhDFR) were significantly up-regulated. The functional analysis of differential genes revealed that LhMYB114 was directly related to anthocyanin accumulation among 19 MYB transcription factors. Furthermore, the qRT-PCR results suggested that their expression patterns were very similar at different developmental stages of the lily bulbs. Virus-induced gene silencing (VIGS) revealed that down-regulation of LhANS-rr1, LhDFR, and LhMYB114 could directly lead to a decrease in anthocyanin accumulation, turning the purple phenotype into a white color. Moreover, this is the first report to reveal that LhMYB114 can regulate anthocyanin accumulation at the mature stage of lily bulbs. The accumulation of anthocyanins is an important sign of lily maturity. Therefore, these findings have laid a solid theoretical foundation for further discussion on lily bulb development in the future.
Identification of Small RNAs Associated with Salt Stress in Chrysanthemums through High-Throughput Sequencing and Bioinformatics Analysis
Authors: Nai Jiefei , Ma Tieming , Liu Yingjie , Zhou Yunwei
Published: February 2023

The Chrysanthemum variety “Niu 9717” exhibits excellent characteristics as an ornamental plant and has good salt resistance. In this study, this plant was treated with 200 mM NaCl for 12 h followed by high-throughput sequencing of miRNA and degradome. Subsequently, the regulatory patterns of potential miRNAs and their target genes were searched to elucidate how Chrysanthemum miRNAs respond to salt. From the root and leaf samples, we identified a total of 201 known miRNAs belonging to 40 families; furthermore, we identified 79 new miRNAs, of which 18 were significantly differentially expressed (p < 0.05). The expressed miRNAs, which targeted a total of 144 mRNAs in the leaf and 215 mRNAs in the root, formed 144 and 226 miRNA–target pairs in roots and leaves, respectively. Combined with the miRNA expression profile, degradome and transcriptome data were then analyzed to understand the possible effects of the miRNA target genes and their pathways on salt stress. The identified genes were mostly located in pathways related to hormone signaling during plant growth and development. Overall, these findings suggest that conserved and novel miRNAs may improve salt tolerance through the regulation of hormone signal synthesis or expression of genes involved in hormone synthesis.
Experiences of Female Nurses’ Parental Leave in Taiwan: A Qualitative Study
Authors: Tseng Ya-Hui , Wu Kuo-Feng , Lin Hung-Ru
Published: February 2023

(1) Background: To counteract the recent severe decline in birthrates in Taiwan, a number of child welfare policies are being promoted. Parental leave is among the most discussed policies in recent years. Nurses are healthcare providers, but their own right to healthcare has not been well investigated and should receive more attention. (2) Aim: This study aimed to understand the experience journey of nurses in Taiwan from considering applying for parental leave to returning to work. (3) Methods: Qualitative design with in-depth interviews was conducted with 13 female nurses from three hospitals in northern Taiwan. (4) Results: Content analysis of the interviews revealed five themes, i.e., considerations for taking parental leave, support received from other parties, life experience during parental leave, concerns regarding the return to the workplace, and preparations for the return to the workplace. Participants were motivated to apply for parental leave due to the lack of help with childcare, the desire to care for their own child, or if their financial situation allowed it. They received support and help during the application process. Participants were happy that they could participate in important developmental stages of their child, but were concerned about disconnect from society. Participants were concerned about not being able to resume work. They successfully returned to the workplace through arranging childcare services, self-adapting and learning. (5) Conclusions: This study can serve as a reference for female nurses considering parental leave and provides insights to management teams for building a friendly nursing workplace and creating mutually beneficial situations.
Effect of Three Weeks of High-Intensity, Long-Term Preoperative Rehabilitation for Esophageal Cancer Patients with Stroke Sequelae Who Were Considered Unfit for Surgery Due to Low Activity: A Case Report
Authors: Kinoshita Tokio , Nishimura Yukihide , Zaiki Rikito , Yasuoka Yoshinori , Umemoto Yasunori , Koike Yumi , Kawanishi Makoto , Tajima Fumihiro
Published: February 2023

Treatment of esophageal cancer is based on tumor-node-metastasis (TNM) classification, and surgical treatment is chosen based on the patient’s ability to tolerate surgery. Surgical endurance partly depends on activity status, with performance status (PS) generally used as an indicator. This report describes a 72-year-old man with lower esophageal cancer and an 8-year history of severe left hemiplegia. He had sequelae of cerebral infarction and a TNM classification of T3, N1, and M0, and was judged ineligible for surgery because his PS was grade three; he underwent preoperative rehabilitation with hospitalization for 3 weeks. He had been able to walk with a cane in the past, but once he was diagnosed with esophageal cancer, he began using a wheelchair and was dependent on assistance from his family in his daily life. Rehabilitation consisted of strength training, aerobic exercise, gait training, and activities of daily living (ADL) training for 5 h a day, according to the patient’s condition. After 3 weeks of rehabilitation, his ADL ability and PS improved sufficiently for surgical indication. No complications occurred postoperatively, and he was discharged when his ADL ability was higher than that before preoperative rehabilitation. This case provides valuable information for the rehabilitation of patients with inactive esophageal cancer.
The Practice of Physical Exercise and Sports in Portuguese Trans Youth: A Case Study
Authors: Oliveira Joana , Frontini Roberta , Jacinto Miguel , Antunes Raul
Published: February 2023

The following case study aims to analyze the experience of a Portuguese trans individual regarding their practice of physical exercise (PE) and sports in Portuguese gyms and sports clubs. A 30-min interview was conducted through the Zoom platform. Before the interview, four questionnaires—Satisfaction with Life Scale (SWLS), Positive and Negative Affect Schedule (PANAS), Hospital Anxiety and Depression Scale (HADS), and EUROHIS-QOL 8-item index—were also applied, all in their Portuguese version. The interview was digitally video recorded after consent was obtained, transcribed verbatim, and subject to thematic analysis. Findings suggest positive values for satisfaction with life and quality of life. The values of positive affect were higher than those of negative affect, and there was an absence of depressive and anxious symptomatology. In the qualitative analysis, mental health was the main motive for the practice, while locker rooms separated by gender and university life were the main barriers mentioned. Mixed changing rooms were identified as facilitators of PE practice. This study highlights the importance of developing strategies for the creation of mixed changing rooms and sports teams in order to promote a comfortable and safe practice for all individuals.
Sustainable Use of CO2 and Wastewater from Mushroom Farm for Chlorella vulgaris Cultivation: Experimental and Kinetic Studies on Algal Growth and Pollutant Removal
Authors: Širić Ivan , Abou Fayssal Sami , Adelodun Bashir , Mioč Boro , Andabaka Željko , Bachheti Archana , Goala Madhumita , Kumar Pankaj , AL-Huqail Arwa A. , Taher Mostafa A. , Eid Ebrahem M.
Published: February 2023

The potential use of carbon dioxide (CO2) and wastewater released from a mushroom farm for the cultivation of Chlorella vulgaris microalga was investigated in this study. For this purpose, a microcontroller-based aided CO2 capture and mixing prototype was constructed for the cultivation of C. vulgaris under varying concentrations of mushroom farm wastewater (0 as control, 50 and 100%). The results showed that the constructed prototype was helpful to maintain desirable CO2 levels (6000 ppm) in the mushroom cultivation chamber with constant CO2 supply to algal culture, i.e., 0.6% at an airflow rate of 50 mL/min. After 16 days of algal cultivation, it was observed that the maximum significant (p < 0.05) algal biomass production of 2.550 ± 0.073 mg/L was recorded in 50% wastewater concentration followed by 100% and control. Also, the maximum removal of selected mushroom farm wastewater pollutants, such as total dissolved solids (84.00 ± 1.37%), biochemical oxygen demand (90.17 ± 2.42%), chemical oxygen demand (91.53 ± 0.97%), total nitrogen (86.27 ± 1.60%) and total phosphorus (94.19 ± 2.33%), was achieved in 50% concentration of wastewater treatment with maximum first-order rate constant (k) values. In addition, the algal growth kinetics results showed that the logistic model fit best compared to the modified Gompertz model, based on selected validation tools, such as experimental vs. predicted values, coefficient of determination (R2 > 0.9938), model efficiency (ME > 0.98) and root mean square error (RMSE < 0.03). The post-harvest characterization of algal biomass revealed that the proximate, biochemical, ultimate elements (carbon, oxygen and nitrogen) and structural properties were significantly higher in 50% treatment than those in 100% and control treatments. Therefore, the findings of this study are novel and provide significant insight into the synergistic use of CO2 and wastewater produced by mushroom farms for algal cultivation and biological wastewater treatment.
Effect of Different Intensities of Aerobic Exercise Combined with Resistance Exercise on Body Fat, Lipid Profiles, and Adipokines in Middle-Aged Women with Obesity
Authors: Oh Du-Hwan , Lee Jang-Kyu
Published: February 2023

We aimed to investigate the effect of different intensities of aerobic exercise (VO2max: 50% vs. 80%) on body weight, body fat percentage, lipid profiles, and adipokines in obese middle-aged women after 8 weeks of combined aerobic and resistance exercise. The participants included 16 women aged >40 years with a body fat percentage of ≥30%; they were randomly assigned to the resistance and either moderate (RME, 50% VO2max, 200 kcal [n = 8]) or vigorous aerobic exercise groups (RVE, 80% VO2max, 200 kcal [n = 8]), respectively. After 8 weeks of exercise, we observed that body weight and body fat percentage decreased significantly in both groups (p < 0.01). The total cholesterol (p < 0.01) and LDL (p < 0.05) levels decreased significantly in the RME group, while triglyceride levels decreased significantly in both groups (p < 0.01). The HDL levels tended to increase only slightly in both groups. The adiponectin levels decreased significantly in the RVE group (p < 0.05), and the leptin levels decreased significantly in both groups (p < 0.05). To prevent and treat obesity in middle-aged women, combined exercise (aerobic and resistance) is deemed effective; additionally, aerobic exercise of moderate intensity during combined exercise could be more effective than that of vigorous intensity.
“Internet+ Recycling” Platform Participation Selection Strategy in a Two-Echelon Remanufacturing Closed-Loop Supply Chain
Authors: Feng Zhangwei , Yang Deyan , Wang Xintian
Published: February 2023

Compared with traditional offline recycling channel, recycling through the “Internet+ recycling” platform has increasingly attracted the academic and practical intention in the past decade because of its accessibility and convenience. To promote the recycling initiatives and construct sustainable operations, how to stimulate supply chain stakeholders participating in the online recycling becomes a challenge issue. This paper considers one supplier, one manufacturer, and one third-party recycler (3PR) in a two-echelon remanufacturing closed-loop supply chain with an “Internet+ recycling” platform, in which consumers can access the online recycling platform and make an appointment for recycling without a physical visit. The manufacturer has three choices: either do not participate or participate with one of two strategies: cost-sharing (CS) or active promotion (AP) strategy. We develop a Stackelberg game model to study the motivation of the manufacturer to participate in the “Internet+ recycling” platform and the influence mechanism of key factors. The key findings include the following: (1) compared with the case without the “Internet+ recycling” platform, when the proportion of cost sharing for the 3PR is low, strategy CS contributes to the improvement of the 3PR’s performance; (2) in the presence of two participation strategies, when the disassembly rate is low enough, the manufacturer prefers strategy AP; otherwise, he selects strategy CS; and (3) a high proportion of cost sharing for the manufacturer or low promotion effort cost can increase the whole profit of the closed-loop supply chain.
Evaluating the Influences of Health Expenditure, Energy Consumption, and Environmental Pollution on Life Expectancy in Asia
Authors: Polcyn Jan , Voumik Liton Chandra , Ridwan Mohammad , Ray Samrat , Vovk Viktoriia
Published: February 2023

This study examines the effects of health expenditure, energy consumption, CO2 emissions, population size, and income on health outcomes in 46 Asian nations between 1997 and 2019. Cross-sectional dependence (CSD) and slope heterogeneity (SH) tests are utilized due to the close linkages between Asian nations as a result of commerce, tourism, religion, and international agreements. The research uses unit root and cointegration tests of the second generation after validating CSD and SH issues. Due to the results of the CSD and SH tests, it is clear that conventional methods of estimation are inappropriate, so a new panel method, the inter autoregressive distributive lag (CS-ARDL) model, is used instead. In addition to CS-ARDL, the study’s results were checked with a common correlated effects mean group (CCEMG) method and an augmented mean group (AMG) method. According to the CS-ARDL study, higher rates of energy use and healthcare spending lead to better health outcomes for Asian countries over the long run. CO2 emissions are shown to be harmful to human health, according to the study. The influence of a population’s size on health outcomes is shown to be negative in the CS-ARDL and CCEMG, but favorable in the AMG. Only the AMG coefficient is significant. In most instances, the results of the AMG and CCEMG corroborate the results of the CS-ARDL. Among all the factors influencing life expectancy in Asian countries, healthcare spending is the most influential. Hence, to improve health outcomes, Asian countries need to take the required actions to boost health spending, energy consumption, and long-term economic growth. To achieve the best possible health outcomes, Asian countries should also reduce their CO2 emissions.
Incarcerated Loved Ones: Building a Community to Support and Advocate on Facebook
Authors: Tadros Eman , Presley Sarah , Gomez Eunice
Published: February 2023

Individuals with an incarcerated loved one are often overlooked when discussing the impacts of incarceration. It can be difficult for these individuals to navigate the criminal justice system, as well as form meaningful connections and obtain support from others that are experiencing a similar situation. Social media allows for connections to be made between individuals in similar situations that might not be geographically close to one another. Specifically, for those with an incarcerated loved one, the Facebook group “Incarcerated Loved Ones” allows for meaningful connection to others who are navigating incarceration. Posts were collected from this Facebook group, with the themes emerging including COVID, information seeking, and advocacy. Findings, as well as future directions, will be discussed.
BiOI-SnO2 Heterojunction Design to Boost Visible-Light-Driven Photocatalytic NO Purification
Authors: Chen Han , Hu Yutao , Ying Zanyun , Xia Yinfeng , Ye Jiexu , Zhao Jingkai , Zhang Shihan
Published: February 2023

The efficient, stable, and selective photocatalytic conversion of nitric oxide (NO) into harmless products such as nitrate (NO3−) is greatly desired but remains an enormous challenge. In this work, a series of BiOI/SnO2 heterojunctions (denoted as X%B-S, where X% is the mass portion of BiOI compared with the mass of SnO2) were synthesized for the efficient transformation of NO into harmless NO3−. The best performance was achieved by the 30%B-S catalyst, whose NO removal efficiency was 96.3% and 47.2% higher than that of 15%B-S and 75%B-S, respectively. Moreover, 30%B-S also exhibited good stability and recyclability. This enhanced performance was mainly caused by the heterojunction structure, which facilitated charge transport and electron-hole separation. Under visible light irradiation, the electrons gathered in SnO2 transformed O2 to ·O2− and ·OH, while the holes generated in BiOI oxidized H2O to produce ·OH. The abundantly generated ·OH, ·O2−, and 1O2 species effectively converted NO to NO− and NO2−, thus promoting the oxidation of NO to NO3−. Overall, the heterojunction formation between p-type BiOI and n-type SnO2 significantly reduced the recombination of photo-induced electron-hole pairs and promoted the photocatalytic activity. This work reveals the critical role of heterojunctions during photocatalytic degradation and provides some insight into NO removal.
Translation, Cross-Cultural Adaptation, and Psychometric Validation of the Positive Body Image among Adolescents Scale (PBIAS) into Spanish and Catalan
Authors: Tort-Nasarre Glòria , Artigues-Barberà Eva , Pollina-Pocallet Mercè , Espart Anna , Roca Judith , Vidal-Alaball Josep
Published: February 2023

The Positive Body Image among Adolescents Scale (PBIAS) explores the factors that bolster and interfere with developing and maintaining a positive body image during adolescence. The aim of this study was to translate, adapt, and validate the PBIAS into Spanish and Catalan. A cross-sectional study was conducted for the instrument’s translation, cross-cultural adaptation, and psychometric validation. A process of translation, back-translation, expert consultation, and piloting was followed. The reliability and statistical validity were evaluated. The Cronbach’s alpha was 0.95 in both the Spanish and Catalan versions. Pearson’s correlation coefficients were statistically significant (r > 0.087) for all items analyzed. The resulting values of the Spanish and Catalan versions indicate a good level of concordance (p < 0.001) with the original questionnaire, the comparative fit index being 0.914 and 0.913, the Tucker–Lewis index being 0.893 and 0.892, the root mean square error of approximation being 1.31 and 1.28, and the standardized root mean square residual being 0.051 and 0.060, respectively. The instrument presents a good level of internal consistency, a high level of reliability, and statistical validity compared to the original instrument. The PBIAS in Spanish and Catalan can be a useful assessment instrument for educators and health professionals in the context of adolescent mental health literacy. This work contributes to the Sustainable Development Goals (Goal 3) of the United Nations 2030 Agenda.
The Association of Perceived Neighbourhood Environment and Subjective Wellbeing in Migrant Older Adults: A Cross-Sectional Study Using Canonical Correlation Analysis
Authors: Liu Yuxi , Liu Huanting , Chen Qin , Xiao Junhui , Wan Chonghua
Published: February 2023

Existing studies often focus on the impact of the neighbourhood environment on the subjective wellbeing (SWB) of the residents. Very few studies explore the impacts of the neighbourhood environment on migrant older adults. This study was conducted to investigate the correlations between perceived neighbourhood environment (PNE) and SWB among migrant older adults. A cross-sectional design was adopted. Data were collected from 470 migrant older adults in Dongguan, China. General characteristics, levels of SWB, and PNE were collected via a self-reported questionnaire. Canonical correlation analysis was performed to evaluate the relationship between PNE and SWB. These variables accounted for 44.1% and 53.0% of the variance, respectively. Neighbourhood relations, neighbourhood trust, and similar values in social cohesion made the most important contributions correlated with positive emotion and positive experience. A link between SWB and walkable neighbourhoods characterized by opportunities and facilities for physical activities with other people walking or exercising in their community, is positively associated with positive emotions. Our findings suggest that migrant older adults have a good walkable environment and social cohesion in neighbourhoods positively correlated with their subjective wellbeing. Therefore, the government should provide a more robust activity space for neighbourhoods and build an inclusive community for older adults.
Spatial Zoning of Carbon Dioxide Emissions at the Intra-City Level: A Case Study of Nanjing, China
Authors: Yuan Yuan , Xu Ping , Zhang Hui
Published: February 2023

With ever-increasing urbanization and industrialization in developing countries, the challenge posed by carbon dioxide emissions (CDEs) has become a hot topic of concern in the realm of sustainable development from a socioeconomic perspective. However, previous studies have only been conducted at macro and meso scales, including at the global, country, and urban levels, and few researchers have delved into the territorial space of urban areas due to a lack of high-precision data. To address this deficiency, we established a theoretical framework to explore the spatial zoning of CDEs based on the newly emerging China high-resolution emission gridded data (CHRED). This study’s innovativeness lies in its provision of a step-by-step process for spatial matching of CDEs based on CHRED in the framework and the construction of square layers to reveal spatial heterogeneity of CDEs at the intra-city level. Taking Nanjing City as the case study area, our findings indicated that CDEs intensity (CDEI) shows an inverted “U-shaped” trend that first increased and then decreased, and finally stabilized from the center to the periphery of the city. With further urbanization and industrialization, the energy consumption sector was found to be the largest contributor to CDEs in Nanjing, and the expanding carbon source zonings will therefore shrink the existing carbon sink zonings. Collectively, these results can provide a scientific reference point to realize China’s “dual carbon” target from the perspective of spatial layout optimization.
Heat Stroke in the Work Environment: Case Report of an Underestimated Phenomenon
Authors: Marrone Maricla , Buongiorno Luigi , Caricato Pierluigi , Pititto Fortunato , De Luca Benedetta Pia , Angeletti Carlo , Sebastiani Gabriele , Cascardi Eliano , Ingravallo Giuseppe , Stellacci Alessandra , Cazzato Gerardo
Published: February 2023

Average global temperatures continue to trend upward, and this phenomenon is part of the more complex climate change taking place on our planet over the past century. Human health is directly affected by environmental conditions, not only because of communicable diseases that are clearly affected by climate, but also because of the relationship between rising temperatures and increased morbidity for psychiatric diseases. As global temperatures and the number of extreme days increase, so does the risk associated with all those acute illnesses related to these factors. For example, there is a correlation between out-of-hospital cardiac arrest and heat. Then, there are pathologies that recognize excessive heat as the main etiological agent. This is the case with so-called “heat stroke”, a form of hyperthermia accompanied by a systemic inflammatory response, which causes multi-organ dysfunction and sometimes death. Starting with a case that came to their attention of a young man in good general health who died while working unloading fruit crates from a truck, the authors wanted to express some thoughts on the need to adapt the world of work, including work-specific hazards, in order to protect the worker exposed to this “new risk” and develop multidisciplinary adaptation strategies that incorporate climatology, indoor/building environments, energy use, regulatory perfection of work and human thermal comfort.
Value Orientations, Personal Norms, and Public Attitude toward SDGs
Authors: Guan Ting , Zhang Qian
Published: February 2023

Improving communication and engagement with the public is vital for implementing sustainable development goals (SDGs). Public attitude toward SDGs can influence this engagement, as people are more likely to accept SDG-relevant information and take actions that are consistent with their own attitudes. This study examines the determinants for individual attitudes in supporting SDGs and further explores the formation of public attitudes toward SDGs, i.e., how public attitude is shaped by the value orientations and norms of the individuals. Using an online survey (n = 3089), we uncovered several important findings: (1) individuals’ altruistic/biospheric value orientations are positively associated with pro-SDG attitudes; (2) personal norms mediate the relationship of individuals’ altruistic values and attitudes; (3) some demographic characteristics (i.e., age, gender, having children) moderate the relationship of people’s value orientations and attitudes; and (4) people’s biospheric values have heterogeneous effects on their pro-SDG attitudes based on education and income. Through these findings, this study enhanced the public’s general understanding of SDGs by providing a holistic analytical framework of public attitude formation on SDGs and uncovering the significant role of value orientations. We further identify the moderating effects of demographic characteristics and the mediating effects of personal norms in the relationship between individuals’ values and attitude on SDGs.
Developing Public Health Promotion Strategies for Social Networking Sites: Perspectives of Young Immigrant Women in Norway
Authors: Agdal Rita , Spjeldnaes Ingrid Onarheim
Published: February 2023

Background: Social networking sites (SNS) have emerged as digital settings for youth participation and health promotion. Understanding the complex dynamic of analog/digital participation has become crucial for settings-based health promotion strategies that aim to enable people to increase control over their health and environments. Previous research demonstrates that SNS influence young people’s health in complex ways, but less is known about how processes related to intersectionality are reflected in digital settings. This study asked the following question: how do young women with immigrant backgrounds experience and navigate SNS and how can this inform settings-based health promotion strategies? Methods: The study included three focus groups with 15 women aged 16–26 years and used thematic content analysis. Findings and conclusion: Young women with immigrant backgrounds reported that transnational networks provided a sense of belonging. However, their presence on SNS strengthened negative social control and had consequences for endeavors to connect with local peers in both digital and analog settings. Both challenges and resources were amplified. The participants reported that sharing strategies to navigate complex networks was useful; they emphasized the importance of anonymous chats, they shared health-related information with extended networks with lower e-literacy, and they saw opportunities for the cocreation of health promotion strategies.
The Impact of Digital Coaching Intervention for Improving Healthy Ageing Dimensions among Older Adults during Their Transition from Work to Retirement
Authors: Santini Sara , Fabbietti Paolo , Galassi Flavia , Merizzi Alessandra , Kropf Johannes , Hungerländer Niklas , Stara Vera
Published: February 2023

Retirement is a critical step in older adults’ lives, so it is important to motivate them to stay physically active, mentally healthy, and socially connected in the transition from work to retirement, including through digital health coaching programs. This study aims to: evaluate the impact of a digital coaching intervention to enhance three healthy ageing dimensions, i.e., physical activity, mental well-being, and socialization of a group of adults near retirement; understand the users’ experience; and identify the system strengths and weaknesses. This longitudinal mixed-methods study, carried out in 2021 in Italy and the Netherlands, enrolled 62 individuals. In the first 5 weeks of the trial, participants used a digital coach with the support of human coaches, and then they continued autonomously for another 5 weeks. The use of the digital coach improved the participants’ physical activity, mental well-being and self-efficacy during the first period and only the physical activity in the second. An effective coaching system should be flexible and attractive. High levels of personalization remain the golden key to aligning the health program to the physical, cognitive and social status of the intended target, thus increasing the user-system interaction, usability, and acceptability, as well as enhancing adherence to the intervention.
Impact of Contextual-Level Social Determinants of Health on Newer Antidiabetic Drug Adoption in Patients with Type 2 Diabetes
Authors: Li Yujia , Hu Hui , Zheng Yi , Donahoo William Troy , Guo Yi , Xu Jie , Chen Wei-Han , Liu Ning , Shenkman Elisabeth A. , Bian Jiang , Guo Jingchuan
Published: February 2023

Background: We aimed to investigate the association between contextual-level social determinants of health (SDoH) and the use of novel antidiabetic drugs (ADD), including sodium-glucose cotransporter-2 inhibitors (SGLT2i) and glucagon-like peptide-1 receptor agonists (GLP1a) for patients with type 2 diabetes (T2D), and whether the association varies across racial and ethnic groups. Methods: Using electronic health records from the OneFlorida+ network, we assembled a cohort of T2D patients who initiated a second-line ADD in 2015–2020. A set of 81 contextual-level SDoH documenting social and built environment were spatiotemporally linked to individuals based on their residential histories. We assessed the association between the contextual-level SDoH and initiation of SGTL2i/GLP1a and determined their effects across racial groups, adjusting for clinical factors. Results: Of 28,874 individuals, 61% were women, and the mean age was 58 (±15) years. Two contextual-level SDoH factors identified as significantly associated with SGLT2i/GLP1a use were neighborhood deprivation index (odds ratio [OR] 0.87, 95% confidence interval [CI] 0.81–0.94) and the percent of vacant addresses in the neighborhood (OR 0.91, 95% CI 0.85–0.98). Patients living in such neighborhoods are less likely to be prescribed with newer ADD. There was no interaction between race-ethnicity and SDoH on the use of newer ADD. However, in the overall cohort, the non-Hispanic Black individuals were less likely to use newer ADD than the non-Hispanic White individuals (OR 0.82, 95% CI 0.76–0.88). Conclusion: Using a data-driven approach, we identified the key contextual-level SDoH factors associated with not following evidence-based treatment of T2D. Further investigations are needed to examine the mechanisms underlying these associations.
End-User Assessment of an Innovative Clothing-Based Sensor Developed for Pressure Injury Prevention: A Mixed-Method Study
Authors: Rêgo Anderson S. , Filipe Luísa , Dias Rosana A. , Alves Filipe S. , Queiroz José , Ainla Alar , Arruda Luísa M. , Fangueiro Raul , Bouçanova Maria , Bernardes Rafael A. , de Sousa Liliana B. , Santos-Costa Paulo , Apóstolo João A. , Parreira Pedro , Salgueiro-Oliveira Anabela
Published: February 2023

This study aimed to evaluate a clothing prototype that incorporates sensors for the evaluation of pressure, temperature, and humidity for the prevention of pressure injuries, namely regarding physical and comfort requirements. A mixed-method approach was used with concurrent quantitative and qualitative data triangulation. A structured questionnaire was applied before a focus group of experts to evaluate the sensor prototypes. Data were analyzed using descriptive and inferential statistics and the discourse of the collective subject, followed by method integration and meta-inferences. Nine nurses, experts in this topic, aged 32.66 ± 6.28 years and with a time of profession of 10.88 ± 6.19 years, participated in the study. Prototype A presented low evaluation in stiffness (1.56 ± 1.01) and roughness (2.11 ± 1.17). Prototype B showed smaller values in dimension (2.77 ± 0.83) and stiffness (3.00 ± 1.22). Embroidery was assessed as inadequate in terms of stiffness (1.88 ± 1.05) and roughness (2.44 ± 1.01). The results from the questionnaires and focus groups’ show low adequacy as to stiffness, roughness, and comfort. The participants highlighted the need for improvements regarding stiffness and comfort, suggesting new proposals for the development of sensors for clothing. The main conclusions are that Prototype A presented the lowest average scores relative to rigidity (1.56 ± 1.01), considered inadequate. This dimension of Prototype B was evaluated as slightly adequate (2.77 ± 0.83). The rigidity (1.88 ± 1.05) of Prototype A + B + embroidery was evaluated as inadequate. The prototype revealed clothing sensors with low adequacy regarding the physical requirements, such as stiffness or roughness. Improvements are needed regarding the stiffness and roughness for the safety and comfort characteristics of the device evaluated.
Mental Health and the COVID-19 Pandemic: Observational Evidence from Malaysia
Authors: Tan Eugenie Sin Sing , Chin Shaun Ashley Fung Xian , Sathapan Manimeyapan S. Palaniappan , Dewi Astrid Disimond , Amini Farahnaz , Bustami Normina Ahmad , Tan Pui Yee , Ho Yu Bin , Tan Chung Keat
Published: February 2023

The interplay of physical, social, and economic factors during the pandemic adversely affected the mental health of healthy people and exacerbated pre-existing mental disorders. This study aimed to determine the impact of the COVID-19 pandemic on the mental health of the general population in Malaysia. A cross-sectional study involving 1246 participants was conducted. A validated questionnaire consisting of the level of knowledge and practice of precautionary behaviors, the Depression, Anxiety, and Stress Scales (DASS), and the World Health Organization Quality of Life—Brief Version (WHOQOL-BREF) was used as an instrument to assess the impacts of the COVID-19 pandemic. Results revealed that most participants possessed a high level of knowledge about COVID-19 and practiced wearing face masks daily as a precautionary measure. The average DASS scores were beyond the mild to moderate cut-off point for all three domains. The present study found that prolonged lockdowns had significantly impacted (p < 0.05), the mental health of the general population in Malaysia, reducing quality of life during the pandemic. Employment status, financial instability, and low annual incomes appeared to be risk factors (p < 0.05) contributing to mental distress, while older age played a protective role (p < 0.05). This is the first large-scale study in Malaysia to assess the impacts of the COVID-19 pandemic on the general population.
Conversion of Polyethylene to High-Yield Fuel Oil at Low Temperatures and Atmospheric Initial Pressure
Authors: Zhang Yuanjia , Chen Xueru , Cheng Leilei , Gu Jing , Xu Yulin
Published: February 2023

The transformation of waste plastics into fuels via energy-efficient and low-cost pyrolysis could incentivize better waste plastic management. Here, we report pressure-induced phase transitions in polyethylene, which continue to heat up without additional heat sources, prompting the thermal cracking of plastics into premium fuel products. When the nitrogen initial pressure is increased from 2 to 21 bar, a monotonically increasing peak temperature is observed (from 428.1 °C to 476.7 °C). At 21 bar pressure under different atmosphere conditions, the temperature change driven by high-pressure helium is lower than that driven by nitrogen or argon, indicating that phase transition is related to the interaction between long-chain hydrocarbons and intercalated high-pressure medium layers. In view of the high cost of high-pressure inert gases, the promotion or inhibition effect of low-boiling hydrocarbons (transitioning into the gaseous state with increasing temperature) on phase transition is explored, and a series of light components are used as phase transition initiators to replace high-pressure inert gases to experiment. The reason that the quantitative conversion of polyethylene to high-quality fuel products is realized through the addition of 1-hexene at a set temperature of 340 °C and the initial atmospheric pressure. This discovery provides a method for recycling plastics by low energy pyrolysis. In addition, we envisage recovering some of the light components after plastic pyrolysis as phase change initiators for the next batch of the process. This method is able to reduce the cost of light hydrocarbons or high-pressure gas insertion, reduce heat input, and improve material and energy utilization.
Pain and Clinical Presentation: A Cross-Sectional Study of Patients with New-Onset Chronic Pain in Long-COVID-19 Syndrome
Authors: Calvache-Mateo Andrés , López-López Laura , Martín-Núñez Javier , Heredia-Ciuró Alejandro , Granados-Santiago María , Ortiz-Rubio Araceli , Valenza Marie Carmen
Published: February 2023

The aim of this study was to evaluate the characteristics of pain (i.e., pain intensity, pain interference, clinical presentation) in Long-COVID-19 patients and compare the location of pain between successfully recovered COVID-19 patients and healthy matched controls. A cross-sectional case-control study was carried out. Long-COVID-19 patients, age- and sex-matched patients with a history of COVID-19 who had successfully recovered, and healthy controls were included. Outcomes included were pain characteristics (Brief Pain Inventory and Short-Form McGill Pain Questionnaire) and clinical presentation (Widespread Pain Index and Euroqol-5 Dimensions 5 Levels Visual Analogue Scale). Sixty-nine patients with Long-COVID-19 syndrome, sixty-six successfully recovered COVID-19 patients, and sixty-seven healthy controls were evaluated. Patients with Long-COVID-19 syndrome showed greater pain intensity and interference. In addition, they showed worse quality of life and greater widespread pain, with the most frequent locations of pain being the neck, legs, and head. In conclusion, patients with Long-COVID-19 syndrome show a high prevalence of pain, characterized by widespread pain of moderate intensity and interference, with the most frequent locations being the neck, legs, and head, significantly affecting the quality of life of these patients.
Urban Tree Canopy and Environmental Justice: Examining the Distributional Equity of Urban Tree Canopy in Guangzhou, China
Authors: Zhuang Yutian , Xie Dixiang , Yu Xijun
Published: February 2023

Rapid urbanization has led to a series of environmental problems that are harmful to the physical and mental health of residents. Not only can increasing urban tree canopy (UTC) improve sustainable city development, but it can also effectively promote the quality of life for residents; however, the uneven spatial distribution of UTC can also bring about social justice problems. Currently, there are few studies related to the justice of UTC distribution in China. Based on this, the paper adopts object-oriented image classification technology to interpret and extract UTC data from satellite images, and it uses house price to explore the justice of the spatial distribution of UTC in the main urban area of Guangzhou from the perspective of environmental justice by ANOVA, Pearson correlation coefficient, and bivariate local spatial autocorrelation. The results show that: (1) There is a significant positive correlation between UTC and house price in the main urban area of Guangzhou, and there are regional differences in the distribution of UTC, with the UTC in the “very high” house price group being significantly higher than that in other groups. (2) The spatial clustering of UTC and house price in the main urban area of Guangzhou is found to be “low-low” and “high-high”; thus, it can be concluded that the spatial distribution of UTC in the main urban area of Guangzhou is uneven. This is an environmental injustice, as the areas with low UTC are spatially clustered in old residential areas, while the areas with high UTC are spatially clustered in commercial housing estates with high house prices. The study shows that urban tree planting should not only focus on quantitative improvement but also on equitable spatial layouts to promote social equity and justice thereby improving the urban ecological environment and promoting healthy urban development.
Optimization of Sperm Cryopreservation Formulation in Portunus trituberculatus
Authors: Chang Le , Lu Chengpeng , Zhu Junquan , Chen Yiner , Wang Chunlin , Mu Changkao , Hou Congcong
Published: February 2023

Portunus trituberculatus is a very important marine economic species, and its aquaculture industry has been developing rapidly. However, the phenomenon of marine wild capture of P. trituberculatus and germplasm degradation has become increasingly serious. It is necessary to develop the artificial farming industry and carry out germplasm resource protection, for which sperm cryopreservation technology is an effective method. This research compared three methods (mesh-rubbing, trypsin digestion, and mechanical grinding) for acquiring free sperm, and the best method was mesh-rubbing. Then, the optimal cryopreservation conditions were selected, and the optimal formulation was sterile calcium-free artificial seawater, the optimal cryoprotectant was 20% glycerol, and the best equilibrium time was 15 min at 4 °C. The optimal cooling program was suspending the straws at 3.5 cm on the liquid nitrogen surface for 5 min and then storing them in liquid nitrogen. Finally, the sperm were thawed at 42 °C. However, the expression of sperm-related genes and the total enzymatic activities of frozen sperm were significantly decreased (p < 0.05), which showed that sperm cryopreservation damaged the sperm. Our study improves the sperm cryopreservation technology and the yield of aquaculture in P. trituberculatus. Additionally, the study provides a certain technical basis for the establishment of a sperm cryopreservation library of crustaceans.
Selected Genetic Factors Associated with Primary Ovarian Insufficiency
Authors: Chen Mengchi , Jiang Haotian , Zhang Chunping
Published: February 2023

Primary ovarian insufficiency (POI) is a heterogeneous disease resulting from non-functional ovaries in women before the age of 40. It is characterized by primary amenorrhea or secondary amenorrhea. As regards its etiology, although many POI cases are idiopathic, menopausal age is a heritable trait and genetic factors play an important role in all POI cases with known causes, accounting for approximately 20% to 25% of cases. This paper reviews the selected genetic causes implicated in POI and examines their pathogenic mechanisms to show the crucial role of genetic effects on POI. The genetic factors that can be found in POI cases include chromosomal abnormalities (e.g., X chromosomal aneuploidies, structural X chromosomal abnormalities, X-autosome translocations, and autosomal variations), single gene mutations (e.g., newborn ovary homeobox gene (NOBOX), folliculogenesis specific bHLH transcription factor (FIGLA), follicle-stimulating hormone receptor (FSHR), forkhead box L2 (FOXL2), bone morphogenetic protein 15 (BMP15), etc., as well as defects in mitochondrial functions and non-coding RNAs (small ncRNAs and long ncRNAs). These findings are beneficial for doctors to diagnose idiopathic POI cases and predict the risk of POI in women.
Experimental Autoimmune Encephalomyelitis of Mice: IgGs from the Sera of Mice Hydrolyze miRNAs
Authors: Nevinsky Georgy A. , Urusov Andrey E. , Aulova Kseniya S. , Ermakov Evgeny A.
Published: February 2023

It was shown that the spontaneous development of experimental encephalomyelitis (EAE) in C57BL/6 mice occurs due to changes in the profile of bone marrow stem cells differentiation. This leads to the appearance of lymphocytes producing antibodies-abzymes that hydrolyze DNA, myelin basic protein (MBP), and histones. The activity of abzymes in the hydrolysis of these auto-antigens slowly but constantly increases during the spontaneous development of EAE. Treatment of mice with myelin oligodendrocyte glycoprotein (MOG) leads to a sharp increase in the activity of these abzymes with their maximum at 20 days (acute phase) after immunization. In this work, we analyzed changes in the activity of IgG-abzymes hydrolyzing (pA)23, (pC)23, (pU)23, and six miRNAs (miR-9-5p, miR-219a-5p, miR-326, miR-155-5p, miR-21-3p, and miR-146a-3p) before and after mice immunization with MOG. Unlike abzymes hydrolyzing DNA, MBP, and histones, the spontaneous development of EAE leads not to an increase but to a permanent decrease of IgGs activity of hydrolysis of RNA-substrates. Treatment of mice with MOG resulted in a sharp but transient increase in the activity of antibodies by day 7 (onset of the disease), followed by a sharp decrease in activity 20–40 days after immunization. A significant difference in the production of abzymes against DNA, MBP, and histones before and after mice immunization with MOG with those against RNAs may be since the expression of many miRNAs decreased with age. This can lead to a decrease in the production of antibodies and abzymes that hydrolyze miRNAs with age mice.