bluuebunny/crossref_metadata_2025 huggingface dataset:
Papillary thyroid cancer with translocation in the TPM3‑NTRK1 gene
Authors: Savchuk M. R. , Shved N. V. , Savelov N. A. , Plaksa I. L.
Published: April 2022

The study objective – to identify specific morphological criteria characteristic of papillary thyroid cancer with translocations in the NTRK genes.

Materials and methods. A retrospective analysis of 130 cases of morphologically confirmed papillary thyroid cancer from the archives of the Moscow City Oncology Hospital No 62, Moscow Healthсare Department was performed. The morphological selection criteria for the immunohistochemical study were: metastatic lesions of the lymph nodes, microcalcifications, extrathyroid spread of the tumor, the presence of a capsule / intratumorous areas of connective tissue, invasion (into the tumor capsule, blood vessels, lymphatic vessels), the presence of intranuclear pseudo-inclusions, the follicle quantity <5 %. Thus, all criteria of thyroid cancer with detected translocation in NTRK genes found in the literature were used. If the tumor met the criteria we performed an immunohistochemistry study with Ventana pan-TRK (EPR17341) Assay antibodies was performed on a BenchMark Ultra immunoassayer. In case of a positive immunohistochemistry reaction, next-generation sequencing on the Illumina HiSeq high-throughput genome-wide sequencing system.

Results. Of the 130 cases analyzed, we identified 10 cases of tumor with histological features characteristic of NTRK positive thyroid cancer. In all cases, the disease was characterized by an indolent course, a slow increase in the tumor over a long time. In patient 5, a tumor of the thyroid gland was combined with extra-organ sarcoma of the retroperitoneal space. The sizes of the tumor node varied from 0.5 . 0.5 cm to 4.0 . 3.0 cm. All patients had metastases to the lymph nodes, in 3 cases the tumor grew beyond the thyroid capsule. According to the results of an immunohistochemical study with antibodies to NTRK (out of 10 applicants) mutation was detected in 1 case. Cytoplasmic expression of varying severity was observed in 95 % of tumor cells. TPM3‑NTRK1 translocation was confirmed by next-generation sequencing.

Conclusion. The presented study demonstrates the possibility of using morphological criteria for detecting thyroid tumors with mutations in the NTRK genes. In the case of a larger sample, it will be possible to identify the structural features of tumors with NTRK mutations. This will clarify morphological criteria and increase the probability of detecting a mutation, which is essential when choosing treatment methods (prescribing targeted therapy).
THE EARLIEST INSECT ENDOPHYTIC OVIPOSITION (EARLY PENNSYLVANIAN, EASTERN UKRAINE)
Authors:   Institute of Geological Sciences of the NAS of Ukraine, Dernov V.
Published: April 2022

The world's oldest insect endophytic oviposition from the deposits of the Mospinka Formation (Upper Bashkirian, Lower Pennsylvanian) of the Donets Basin is described in the paper. There is little information on Carboniferous endophytic oviposition of insects. The earliest insect endophytic oviposition specimens are specimens from the Upper Moscovian and the uppermost part of the Gzhelian of Germany, as well as the uppermost Gzhelian of France and the USA. The endophytic oviposition described in this article is almost 10 million years older than specimens from the Upper Moscovian of Germany. The studied material was collected on five localities and four stratigraphic levels. The study area is the upper reaches of the Velyka Kamyanka River (southern part of the Luhansk Region, Ukraine). The rocks with the studied oviposition were formed in shallow marine, lagoonal and lacustrine environments. Insect damages were noted on leaves of arborescent lycopsids and cordaitaleans, and pteridosperm rachises. The above mentioned oviposition belongs to the following damage types sensu Labandeira et al., 2007: DT76, DT100, DT101 and DT175. The studied oviposition occurs mainly in sediments formed in the environments of a highly watered coastal alluvialdeltaic lowland with associations of the semi-aquatic sphenopsids (shores of lakes, rivers, and freshened lagoons), predominantly arborescent lycopsids (swampy areas), as well as pteridosperms on the elevated areas of accumulation plain. The most likely producers of endophytic oviposition are representatives of Odonatoptera, Palaeodictyopteroidea, and Orthoptera. The new findings substantially supplement the fossil record of insect endophytic oviposition.
INVESTIGATION OF SECONDARY TRANSFORMATIONS WITHIN LOWER CARBONIFEROUS ROCKS OF THE CENTRAL PART OF THE DNIEPER-DONETS DEPRESSION
Authors:   Taras Shevchenko National University of Kyiv, Tunik O.
Published: April 2022

The article describes the main study results of the secondary transformations effect on the lower Carboniferous shales at depths exceeding 3 000 m. Using silicate, energy-dispersive X-ray fluorescence analysis and electron microscopic studies, the structure and the main rock-forming components in core samples were studied. The study showed the presence of both background and imposed epigenetic changes in the studied core samples. The intensity and variety of the secondary transformations increase with depth. It has been confirmed that compaction of clay rocks reduces their porosity and improves sealing properties. The presence of scattered siltstone material does not affect the value of porosity. Porosity increases with increasing of kaolinite content which occurred in result of secondary imposed processes. According to the composition of mineral paragenesis, it was found that the studied shales are at the stage of mesocatagenesis. The further research development for the purpose of approximation of microscopic and geochemical data by means of the extended well logging suit was outlined.
Comparative analysis of the linear and nonlinear rules of mixtures in modeling the stress state of a half-space
Authors:   State University of Telecommunications, Yakovenko N. D. , Chervinko O. P. ,   S.P. Timoshenko Institute of Mechanics NAS Ukraine, Yakymenko S. M. ,   Central Ukrainian National Technical University
Published: April 2022

In the present work we solve the axially symmetric problem of a half-space under thermal loading. The statement of the problem includes: Cauchy relations, equations of motion, heat conduction equation, initial conditions, thermal and mechanical boundary conditions. The thermomechanical behavior of an isotropic material is described by the Bodner–Partom unified model of flow generalized in the case of microstructure influence on inelastic characteristics of steel. To determine the parameters of the model corresponded to yield stress and yield strength the mixture rule is utilized. The problem is solved with using the finite element technique. The numerical realization of our problem is performed with the help of step-by-step time integration. Equations of the evolution for the inelastic flow model are integrated by the second-order Euler implicit method. The equations of motion are integrated by the Newmark method, whereas the heat-conduction equation is integrated by the first-order implicit method. We use quadrangular isoparametric elements. The parameters of a fine grid are chosen with the help of the criterion of practical convergence of the solutions. The stress state taking into account linear and nonlinear rules of mixtures is described.
Prospective directions for innovative development strategies in pharmacy in the military health system of the Russian Federation
Authors: Miroshnichenko Yuri V. , Ivchenko Evgeniy V. , Kononov Vladimir N. , Golubenko Roman A. , Ovchinnikov Dmitrii V. , Enikeeva Rimma A. , Shcherba Maria P. , Merkulov Andrey V. , Mavrenkov Eduard M.
Published: April 2022

Strengthening the readiness of the medical service system of the Armed Forces of the Russian Federation to fulfill given tasks is possible only with innovative development, which constitutes the scientific and methodological basis for the modernization of military healthcare. In this connection, researchers at the pharmacy department of the Kirov Military medical Academy considered the development of kits and equipment, medical equipment, mobile and special medical equipment, and pharmaceutical technologies as the absolute priority of their scientific development. The requirements for the equipment of different units and military medical organizations both at present conditions and in future wars include high level of mobility, shortened deployment (curtailment) terms, autonomy, increased operational efficiency of units, etc. The realization of these requirements is possible if certain issues are solved, primarily aimed at the digitalization of medical supply system of military forces, improvement of basic equipment, and entering new developments in the field of pharmaceutical technology into the medical service of the Armed Forces of the Russian Federation. The article presents the perspective directions of the innovative development of the medical supply system of the Armed Forces, which is the most important element of military healthcare. Results of the activity such as creation and acceptance for the supply of mobile units for production, accumulation (storage), delivery, distribution of medical gaseous oxygen, registration of medicinal products (medical oxygen, 93%), and acceptance of the corresponding pharmacopoeial article are already represented. Still at the development stage, there are innovative sets of service equipment of airborne troops, sterilization, and distillation unit, allowing the use of water from natural surface sources of domestic and drinking water supply, etc.
Academician of the Russian academy of sciences Yu.L. Shevchenko and his contribution to the development of the Military Medical Academy and health care of the Russian Federation (on his 75th anniversary)
Authors: Kryukov Evgeniy V. , Khubulava Gennady G.
Published: April 2022

April 7, 2022 marks the 75th anniversary of the outstanding national cardiac surgeon, Academician of the Russian Academy of Sciences Yuri Leonidovich Shevchenko. Yu.L. Shevchenko graduated from the Military Medical Academy in 1974 and after a year of servicing in the military he returned to the Academy, where he worked for a quarter of a century, having done his way up from a senior resident of the clinic of the Hospital Surgery Department to the Head of the Academy. During his leadership of the Academy (19922000) he did a lot not only for its preservation but also for its development and re-equipment. Great attention was paid to the international scientific cooperation, increasing the public prestige of the Academy and preserving its historical heritage: the academician B.V. Petrovsky medal "For outstanding surgeon in the world" was established; the monument "Military medics who died in wars" was opened; the Institute of honorary doctors and academicians of the Military Medical Academy was restored; the Academy was included in the State List of especially valuable objects of cultural heritage of Russian Federation. Internship and certification of graduates were introduced in the educational and scientific activities, and the Academy scientific schools were preserved and strengthened. As the Minister of Health of Russian Federation Yu.L. Shevchenko participated in the development of a number of federal target programs: justified and implemented in practice the territorial system of health care, also he was one of the initiators of creation and formation of health insurance system. Yu.L. Shevchenko contributed greatly to the development of Russian and world cardiosurgery. His works were a milestone in solving the problems of surgical treatment of infectious endocarditis. For the first time, the "sanitizing effect" of artificial circulation was theoretically substantiated and proved in practice, the optimal values of perfusion during surgical treatment of generalized forms of infection were developed, and the integral approach to sanitation of the heart chambers was substantiated. Since 2002 Yu.L. Shevchenko has been the head of Pirogov National Medical-Surgical Center. For his great contribution to the development of medical science academician Yu.L. Shevchenko was awarded the State Prize and honorary titles of Honoured Scientist and Honoured Physician of Russia, he was awarded the Order of Merit for the Motherland of 4th Class and the Order of Honour.
Approaches to isolating and purifying nucleic acids from blood for genotyping human leukocytic antigen
Authors: Baranov Oleg A. , Bayran Dmitry A. , Markin Ilya V. , Shchelkanova Elena S. , Zhurbin Evgeny A.
Published: April 2022

Donorrecipient histocompatibility results from the presence on cell membranes of the main protein complex of histocompatibility and is a key condition for successful transplantation of cells, tissues, and organs. To determine histocompatibility, human leukocyte antigen is genotyped, and its accuracy relied significantly on the quality and quantity of nucleic acids obtained from the biomaterial. In laboratory practice, the most pure and intact deoxyribonucleic and ribonucleic acids are extracted from the blood; however, the choice of an accessible, effective, time-efficient, and viable method for their production remains challenging. The methods of isolation and purification of nucleic acids from the blood include organic extraction, salting out, and use of spin columns and magnetic particles (bidids), and their advantages and disadvantages, efficiency indicators, practicality, and cost were compared. The selection of the peripheral blood as a source of genetic material for genotyping human leukocyte antigen is justified. Experimental data comparing the pricequality ratio of commercial protocols for the extraction of deoxyribonucleic and ribonucleic acids from blood were analyzed. Prospects of modification of procedures for isolation and purification of nucleic acids from biomaterial for sequencing of genes of human leukocyte antigen classes I and II to increase efficiency of high-tech care are evaluated. Generally, the need for affordable and effective protocols for the extraction of deoxyribonucleic and ribonucleic acids from minimal biomaterial volumes stimulates the optimization and modification of existing protocols and the creation of new methods on new physicochemical principles.
Lumbosacral dorsopathies: modern aspects of diagnosis and pharmacological treatment
Authors: Zhivolupov Sergey A. , Kozhevnikov Evgeny Y. , Samartsev Igor N. , Rashidov Nariman A.
Published: April 2022

Lumbosacral dorsopathy is the most common diagnosis in patients with lower back pain. Issues of conservative treatment of lumbosacral dorsopathies are considered based on the analysis of scientific materials contained in large databases such as PubMed, Cochrane library, MEDLINE, and eLibrary. From all publications, 40 of the most relevant works were selected and considered in detail, and key figures and short conclusions for each work are displayed in a table. Symptoms of lumbosacral dorsopathies often have an extremely negative effect on the patients ability to work and quality of life; therefore, effective and prompt treatment is important. Given the complex and multifactorial pathogenesis and multitudes of options for lumbosacral dorsopathies, conservative treatment should be complex and individualized, and drugs should have a high safety profile, especially those used in the treatment of chronic diseases. These requirements are met by some nonsteroidal anti-inflammatory drugs and co-analgesics, such as chondroprotectors and B vitamins. For the treatment of acute and exacerbated lumbosacral dorsopathies, the local use of hormonal drugs, anticonvulsants, antidepressants, muscle relaxants, etc., is possible, while treatment dosage and duration should be strictly limited. The local use of hormonal drugs and hodroprotectors such as therapeutic and diagnostic blockades is justified in the absence of contraindications and availability of opportunities, which helps reduce the likelihood of systemic adverse reactions and provides greater bioavailability.
Features of the military population health in the arctic zone
Authors: Dybin Alexey S. , Poteryaev Alexander E. , Kuznetsov Sergey A. , Luchnikov Eduard A. , Mavrenkov Eduard M. , Menshikova Larisa I.
Published: April 2022

Тhe study aimed to assess the population health of military personnel serving under contract in the Arctic region and to identify its features. On the basis of statistical reports on form 3/med., relative indicators characterizing the health status of military personnel are calculated. Polynomial trends of the second degree and absolute increase/decrease values were calculated for dynamic analysis. The determination factor was used to estimate the significance of the changes. Quantitative data are presented as arithmetic mean with 95% confidence interval. For comparison, Students t-test and the Man Whitney test were used. Indicators of the health status of the military personnel in the Arctic region have specific features. The primary incidence rate among all categories of military personnel was 452.28, and the total incidence was 993.28. The hospitalization rate coefficient was equal to 154.16, the incidence with temporary disability coefficient was 3912.29, the dismissal rate was 10.61 , and the mortality rate was 118.4 per 100 thousand people. The health coefficient of the sample population was 43.67 units, with a significant downward trend. Respiratory (50%), musculoskeletal (13%), skin and subcutaneous (7%), digestive (6%), and circulatory (6%) diseases account for the highest rates of primary morbidity, whereas the rates of skin diseases decreased and the proportion of musculoskeletal diseases increased. An assessment of the primary morbidity dynamics revealed a significant increase among respiratory, digestive, musculoskeletal, and connective diseases and a significant trend toward a decrease in the rate of primary morbidity of mental and behavioral disorders and a decrease in the number of injuries, poisoning, and some other consequences of external causes. The health of the military population in the Arctic region has specific features. Climatogeographic and heliophysical factors of the Far North significantly influence the health of military personnel under contract in this region. Thus, targeted preventive measures, including improving the health-promoting behaviors of military personnel under adverse environmental conditions, are necessary.
Modern methods of detection and identification of microbial toxins that inhibit protein synthesis in cells
Authors: Miteva Olga A. , Yudina Nadezhda S. , Myasnikov Vadim A. , Stepanov Alexander V. , Chepur Sergey V.
Published: April 2022

Pathogenic microorganisms and products of their metabolism, namely, bacterial protein exotoxins, are considered one of the main sources of biological threat. Microbial toxins are highly active and extremely dangerous to humans. Determining trace amounts of such compounds remains relevant in healthcare and biological protection sector. Timely qualitative- and quantitative-specific indication of biotoxins is a key component in the diagnosis and implementation of therapeutic and preventive measures. Pathogenic microorganisms and products of their metabolism, bacterial protein exotoxins, are considered one of the main sources of biological threat. Microbial toxins are highly active and extremely dangerous to humans. Determining trace amounts of such compounds remains relevant in healthcare and biological protection sector. Timely qualitative- and quantitative-specific indication of biotoxins is a key component in the diagnosis and implementation of therapeutic and preventive measures. The current state and prospects of development in formulating specific indications of microbial toxins that disrupt protein synthesis in cells are analyzed. Modern ideas about the structure and mechanism of action of these toxins are briefly presented. Possibilities were considered, the advantages and disadvantages of classical traditional and modern innovative methods for identifying bacterial toxins that inhibit protein synthesis in cells were compared, and classifications were provided. Examples of the use of various approaches to identify the most significant representatives of this group in both clinical material and in environmental objects, including regulated ones, were given. The review also listed modern domestic and foreign developments in formulating specific indications of microbial toxins inhibiting protein synthesis. The review summarizes the results of studies to determine the current directions in the development of tools and methods for rapid specific indication of microbial toxins. The main trends in the creation of new methods of toxicological screening as part of an effective national system for monitoring biological threats were analyzed. Prospects for the development and introduction to the market of domestic test systems and automatic analysis platforms for the detection of bacterial toxins in environmental objects and biological material were determined.
Reconstruction of the ligamentous-tendinous complex of the knee joint, ensuring its varus stability
Authors: Khominets Vladimir V. , Kudyashev Aleksey L. , Gaivoronskiy Ivan V. , Bazarov Ivan S. , Grankin Aleksey S. , Semenov Aleksey  A. , Konokotin Dmitri A.
Published: April 2022

А new technique for anatomical reconstruction of the ligamenttendon complex of the knee joint, which restores its varus stability, in patients with multiligament injury is substantiated. The technical feasibility, safety, and effectiveness of the proposed technique were evaluated in an anatomical experiment. The study was performed on eight lower extremities of four unfixed corpses. After simulating the varus instability of the knee joint, the plasty of the ligamenttendon complex providing the varus stability of the knee joint was performed according to the proposed technique. The essence of the proposed technique is the simultaneous restoration of the peroneal collateral ligament, popliteal tendon, and poplitealperoneal ligament using a single autograft of a semitendinosus muscle. After simulating the surgical procedure on anatomical specimens, the varus stability of the knee joint was evaluated based on the results of functional radiography. The safety of the experimental procedure was evaluated based on the results of the applied morphometric study of the distance from the reconstructed elements of the posterolateral corner of the knee joint to the popliteal artery and the common peroneal nerve in the 90-degree flexion position in the knee joint. Accordingly, the formed bone tunnels for a single autograft were located at a safe distance from the elements of the neurovascular bundle of the popliteal fossa. The technical possibility of reconstruction of the collateral peroneal ligament, popliteal tendon, and poplitealperoneal ligament according to the proposed technique. After simulating the reconstruction of elements of the posterolateral corner of the knee joint, varus stability was objectified by a series of functional X-ray studies of the anatomical specimens. The results indicate the technical feasibility, effectiveness, and relative safety of the proposed method of reconstruction of the ligamenttendon complex of the posterolateral corner of the knee joint providing the varus stability of the knee joint in patients with multiligament injury.
Variants of facial nerve neurotization
Authors: Gaivoronsky Alexey I. , Skaliitchouk Bogdan V. , Vinogradov Vyacheslav V. , Alekseev Dmitriy E. , Svistov Dmitriy V.
Published: April 2022

This study presents facial nerve neurotization, a common method of surgical treatment of facial muscle paralysis. In this surgical procedure, a trunk or some portions of individual fibers are sewn to an intact nerve-neurotizator to the injured facial nerve that can act as sublingual, masseteric, phrenic, accessory, glossopharyngeal nerves, as well as the descending branch of the sublingual nerve and anterior branches of the C2C3 cervical spinal nerves. Often, neurosurgeons combine various donor nerves and autotransplanting inserts for better results. The main stages of neurotization of the facial nerve includes isolation and transection of the facial nerve, isolation and transection of the trunk or separate fibers of the neurotizer, and nerve suturing in an end-to-end or end-to-side fashion. Facial cross-plasty, the most innovative method of facial nerve neurotization, should be carefully performed, during which an anastomosis is performed between the damaged and intact facial nerves using autotransplantation inserts from the calf nerve or from a free muscle graft, including a tender muscle and an anterior branch of the locking nerve. Recovery of facial nerve function and regression of characteristic symptoms takes time and specialized recovery treatment. Generally, among the lesions of the cranial nerves, injuries and diseases of the facial nerve rank first and are one of the most common pathologies of the peripheral nervous system. The clinical picture of facial nerve injuries in various origins is quite monotonous and manifested by persistent paralysis or paresis of the facial muscles. Various highly effective techniques are aimed at restoring the function of the facial nerve and facial muscles. Many conservative and operative methods of treating facial nerve neuropathy have been presented in the modern medical literature. However, all methods of facial nerve neurotization have several disadvantages, and the leading ones are the inability to achieve 100% efficiency and development of one degree or another neurological deficit.
Use of a local hemostatic agent based on chitosan and external compression of the abdominal area to control intra-abdominal bleeding
Authors: Golovko Konstantin P. , Samokhvalov Igor M. , Grishin Maxim S. , Nosov Artem M. , Yudin Andrey B. , Kovalevsky Arkady Y. , Bagnenko Andrey S. , Kovalishin Ivan M.
Published: April 2022

The paper presents the intermediate results of an experimental study of three samples of chitosan-based local hemostatic agent in a model of intense intra-abdominal bleeding in an average-sized experimental animal (a Soviet Chinchilla rabbit) with a standard wound on the parenchymal organ (liver) in combination with external compression of the abdominal area to achieve hemostasis in the acute phase of the experiment. At the first stage, nine experimental groups and one control group, with three biological objects each, were involved (n = 30). The composition and properties of laboratory preparations of hemostatics were different from the concentration of the main component. To select the optimal sample of a local hemostatic agent with the least local irritant effect on the organs and structures of the abdominal cavity, three experimental groups with three animals each were formed at the second stage, without modeling blood loss (n = 9) with follow-up from 24 to 72 h. The studied drugs have high hemostatic activity in the model of intense intra-abdominal bleeding with follow-up periods from several hours to 3 days. Moreover, intraperitoneal administration of hemostatic agent samples does not lead to a pronounced local irritant effect. However, during the first 180 min of the experiment, only one fatal outcome was recorded in a group of animals with 15% chitosan in the studied drug because of a technical error in modeling the source of bleeding. External compression of the abdominal area demonstrated its effectiveness as an auxiliary technique for temporary control of the intra-abdominal source of bleeding in the experimental model. The objectification of the obtained results was achieved through a control sectional study and laboratory screening of peripheral blood indicators of experimental animals at different stages of the experiment. To further evaluate the hemostatic activity of chitosan-based drugs, creating an experimental model using a large experimental animal is necessary.
History and continuity of the first therapeutic departments at the Military Medical Academy
Authors: Ovchinnikov Dmitrii V.
Published: April 2022

The problem of the lack of a statute on the creation of the medicalSurgical Academy arises every time the authors start discussing the history of the medicalSurgical Academy or its subdivisions. There are also misunderstandings in the issue of internal medicine teaching and creation of therapeutic departments. The contents of several historical and archival documents and earlier publications about the period of formation and development of the first therapeutic departments of the Academy (the last third of the 18th to the first half of the 19th century) were clarified. The article also analyzed the general anniversary editions of the academy, dissertations defended on the history of the departments, historical collections, and documents published in the Complete Collection of Laws of the Russian Empire, stored in the archives (Central State Archive of Ancient Acts, Russian State Archive of the Navy, etc.). The teaching of internal medicine started in October 1767 by F.T. Tikhorsky. From 1786, P. Hoffman continued to teach pathology, therapy, and medical practice at the Chief medical School. G.I. Bazilevich was appointed the second professor of pathology and therapy at the MedicoSurgical School. I.A. Smelovsky became the first professor after the college was renamed into academy. I.P. Frank, who created the second therapeutic department, started a direct historical branch of the therapeutic department until 1931, when the Department of the Private Pathology and Therapy merged with the united therapeutic department, and the Department of Propaedeutics of Internal Diseases emerged from it. From 1848 to 1924, the Department of General Therapy, attached to the first department, existed. In 1806, thanks to I.P. Frank, the second therapeutic department was founded, which existed since 1810 and is now the Department of General Therapy. Initially created as the Department of Hospital Therapy Clinic and renamed in 1965 at the insistence of N.S. Molchanov into the Department of Therapeutic Advancement of Physicians № 1 was actually created by the Decree of January 19th, 1842, simultaneously with the appointment of its first full-time head Prof. O.I. Myanovsky.
Strategy for the development of specialized medical care for patients with urolithiasis in the armed forces of the Russian Federation
Authors: Sergoventsev Alexander A. , Kryukov Evgeny V. , Protoshchak Vladimir V. , Paronnikov Mihail V. , Savchenko Igor F. , Orlov Dmitriy N.
Published: April 2022

The development of a system of therapeutic, diagnostic, and organizational measures to improve specialized medical care for members of the Armed Forces of the Russian Federation with urolithiasis is scientifically substantiated. The study was conducted in 3 stages. At the first stage, the principal functions of the system of specialized urological care for patients with urolithiasis were singled out, and possible ways of their realization were determined. At the second stage, planning options for the system were formulated and described. In the third stage, the criteria for analyzing the system, estimation of their superiority, and scientific substantiation of the rational concept were elaborated. Three systems of providing specialized urological care to patients with urolithiasis with the conditional names, including proposed, real, and adaptive, were formulated and put forward. The proposed system was based on the principle of the maximum volume of specialized medical care according to the urology profile in the district hospitals of each military district and hospital of the Northern Fleet. The real system has a four-stage structure, which was based on staged treatment of patients with urolithiasis receiving the maximum treatment in organizations of central subordination. In the adaptive system, its conceptual basis is the central military medical organizations, and the volume of care in the remaining hospitals is determined by the chief urologist of the Ministry of Defense of the Russian Federation. Using hierarchy analysis, a pairwise comparison of each concept was performed, and the proposed system has the highest priority index. Generally, to improve the specialized care for members of the Armed Forces of the Russian Federation with urolithiasis, we have developed and scientifically proved the system of the organizational treatment and diagnostic measures that assume the decentralization and standardization of the urological care for patients with urolithiasis, staff reorganization, provision of modern equipment, professional training of the medical staff, improvement of research potential, and interaction with medical institutions of the civilian population.
Combined diseases of endocrine glands in the clinic of nervous diseases
Authors: Protopopov K. I.
Published: April 2022

The endocrine glands play an enormous role in the life of the organism in general and in its neuropsychic activity in particular. The glands are in a certain interaction with each other (chemical correlation), their secrets mutually reinforce each other or neutralize; thus, the loss of one of these organs (resp. secrets) from the system or a change in their activity leads, in addition to the loss of a function specific to this organ, to a certain change in the entire system. The clinic gives us a whole series of examples confirming the propositions worked out by physiology.
Chronicle and mix. Volume XXIII, № 1-2 (1916)
Authors: Baklushinsky I. D.
Published: April 2022

In Moscow, in the premises of the 1st Red Cross Hospital for mentally ill soldiers, the final exams of the first courses of sisters of mercy for the care of mentally ill soldiers were held. Courses opened two months ago. The courses were taken by women with education not lower than average. Particular attention was paid to the practical training of future sisters of mercy. The head of the courses was Privatdozent of Moscow University, Doctor of Medicine A. L. Lyubushin
Chief editor of the Kazan journal "Neurological Bulletin", Honored Scientist of the Republic of Tatarstan, prof. M.F. Ismagilov
Authors: Badalyan Levon O.
Published: April 2022

The editorial board of the S.S. Korsakov Journal of Neuropathology and Psychiatry welcomes the resumption of the publication of the Neurological Bulletin, founded by Academician V.M. Bekhterev!

Your Vestnik is nine years older than Korsakov's journal. For many years before the October Revolution, our journals were published in parallel in Kazan and Moscow, Moscow and Kazan. In 1917-1918. almost all magazines were closed. In 1925, the Journal of Neuropathology and Psychiatry began to be published again. The Kazan Medical Journal was published.
Dependence of spectral power of EEG rhythms on menstrual cycle phases in women suffering from epilepsy
Authors: Karlov V. A. , Vlasov P. N. , Eligulashvili T. S.
Published: April 2022

The effect of menstrual cycle on EEG parameters is investigated in norm, in epilepsy becoming acute in menstrual and perimenstrual periods (catamenial), and in epilepsy whose exacerbation is not connected with menstrual cycle. The ten dencies to the decrease of spectral power in cycle luteal phase in healthy women and women suffering from epilepsy are mentioned. The primary role of spectrum low-frequency components and total power level in neurodynamics of catamenial epilepsy is shown.
Paroxismal states in neuromuscular diseases
Authors: Lobzin V. S.
Published: April 2022

The diagnosis, clinical picture, problems of patogenesis and emergency medical service in myasthenia and Lambert-Itons syndrome are considered, for the most part in crises with disorders in respiration and cardiac activity, crisis states in Tomsens myotonia and dystrophyc myotonia as well as in the large group of so-called dispotassemic paralyses, thyrotoxic myopathy and some other rarer forms of neuromuscular diseases.
To the 100th anniversary of the discovery of the two-neuron pathway of the parasympathetic nerves of the heart
Authors: Volkova I. N. , Zefirov А. L.
Published: April 2022

100 years ago (1894), an article by V.V. Nikolaev appeared in the journal Neurological Bulletin under the modest title: About the end of n. vagi in the heart." The author performed his research in the physiological laboratory of Kazan University, led by Professor N.A. Mislavsky.
Achatina fulica infectado por Angiostrongylus cantonensis en Napo, Ecuador
Authors: Solórzano Álava Luis , Hernández Álvarez Hilda , Bedoya Pilozo Cesar , Rodríguez Misladys , Rojas Rivero Lázara
Published: April 2022

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Clonamiento, expresión y seroreactividad de la proteína recombinante de ensamblaje de lipopolisacáridos – D (LptD) de Bartonella bacilliformis
Authors: Flores-Nuñez Astrid , Ventura Gladis , Bailon Henri , Marcelo Adolfo , Sandoval Gustavo , Padilla-Rojas Carlos
Published: April 2022

Objetivo. Evaluar in silico y a nivel serológico el potencial antigénico del dominio extracelular recombinante de la proteína de ensamblaje de lipopolisacáridos - D (LptD) de Bartonella bacilliformis (dexr_LptD). Materiales y métodos. Mediante el análisis in silico se realizó la selección de una proteína de B. bacilliformis con potencial antigénico e inmunogénico. El gen de la proteína seleccionada se clonó en Escherichia coli TOP10 y se expresó en Escherichia coli BL21 (DE3) pLysS. La proteína recombinante fue expresada usando isopropil-β-D-1-tiogalactopiranósido (IPTG) y se optimizaron las condiciones de inducción. Por último, se purificó con resina Ni-IDA (His60 Ni Superflow) y se realizó un ensayo de Western Blot. Resultados: In silico, la proteína seleccionada fue LptD por estar localizada en la membrana externa y ser antigénica e inmunogénica. Las condiciones optimizadas para la inducción del dexr_LptD fueron 0,5 mM IPTG, 16 h, medio TB (Terrific Broth), etanol al 3% (v/v), 28 ºC, OD600: 1-1,5 y 200 r.p.m. La purificación se realizó en condiciones denaturantes a pequeña escala y se obtuvo 2,6 μg/mL de dexr_LptD parcialmente purificada. El ensayo de Western Blot mostró una reacción positiva entre los sueros provenientes de pacientes con la enfermedad de Carrión y dexr_LptD, ello evidencia la antigenicidad del dexr_LptD. Conclusiones. El dexr_LptD muestra antigenicidad in silico y a nivel serológico, estos resultados son base para posteriores estudios sobre candidatos vacunales contra la enfermedad de Carrión.
COVID-19 SALGINININ S&P 500 ENDEKSİ OYNAKLIĞI ÜZERİNDEKİ ETKİSİ
Authors: ÜRKMEZ Emre
Published: April 2022

Yeni tip bir koronavirüs hastalığı (COVID-19) yakın tarihte yalnızca benzeri görülmemiş bir sağlık krizi değil, aynı zamanda küresel ekonomiyi etkisi altına alan en maliyetli pandemilerden biri olması bekleniyor. Son yapılan çalışmalar, COVID-19 salgınının bulaşıcılık etkisi ile finansal piyasaların oynaklığını arttırdığını gösteriyor. Bu çalışma, COVID-19 salgınının S&P 500 endeksi hisse senedi getirilerinin oynaklığı üzerindeki etkisini incelemeyi amaçlamaktadır. Bu amaçla hisse senedi getirilerinin oynaklığı 21 Ocak 2020 ile 09 Nisan 2021 dönemleri arası günlük veriler kullanılarak GARCH-X modeli ile analiz edilmiştir. GARCH-X modeli COVID-19 faktörünü koşullu ortalama ve varyans denklemlerine bir dışsal değişken olarak ilave edilmesine izin vermektedir. Bulgular, ABD’de günlük olarak bildirilen COVID-19 vakalarının kısa dönemde hisse senedi getirileri üzerinde istatistiksel olarak anlamlı bir etkiye sahip değildir. Ancak, sonuçlar COVID-19 salgınının hisse senedi getirilerinin oynaklığı üzerinde pozitif ve istatistiksel olarak anlamlı bir etkiye sahip olduğunu göstermektedir. Genel olarak, sonuçlar COVID-19 salgınının hisse senedi getirilerinin oynaklığı üzerinde ciddi bir etkisi olabileceğine işaret etmektedir.
TUBULİN-SPECİFİC CHAPERONE D (TBCD) GENİNDE YENİ BİR MUTASYON TESPİT EDİLEN PEBAT SENDROMLU OLGU
Authors: Elmas Muhsin , Göğüş Başak , Bükülmez Ayşegül , Solak Mustafa
Published: April 2022

Tubulinopatiler, tubulin izoformlarını kodlayan çeşitli genlerde meydana gelen mutasyonların sebep olduğu nörogelişimsel / nörodejeneratif hastalıklar ailesini tanımlamaktadır. Tubulin katlanmasına ve polimerizasyonuna katılan beş tubulin spesifik şaperondan birini kodlayan tubuline özgü şaperon D (Tubulin-Specific Chaperone D, TBCD)'deki mutasyonlar, nadir görülen nörogelişimsel “ensefalopati, ilerleyici, erken başlangıçlı ve beyin atrofisi ve ince korpus kallosum” ile karakterize PEBAT sendromuna neden olur. Söz konusu sendrom, erken başlangıçlı kortikal atrofi, sekonder hipomyelinasyon, mikrosefali, gelişimsel gecikme, mental retardasyon, epilepsi, optik atrofi, spastik kuadripleji ve beyin manyetik rezonans görüntülemesinde ince korpus kallozum görüntüsü ile karakterize nörogelişimsel ve nörodejeneratif özelliklere sahiptir. PEBAT sendromuna neden olan TBCD'deki mutasyonlar otozomal ressesif kalıtım paterni ile geçiş göstermektedir. Dolayısıyla akraba evliliği söz konusu mutasyon için önemli bir risk faktörüdür. 5 yaşındaki erkek hasta epilepsi ve nöromotor gerilik şikayetiyle tarafımıza başvurmuştur. Yapılan tüm ekzom dizileme analizinde TBCD geninde homozigot [c.230A>G (p.His77Arg) (p.H77R)] mutasyonu saptandı.
KRONİK LENFOSİTİK LÖSEMİ VAKALARINDA HÜCRE YÜZEY ANTİJEN EKSPRESYONLARININ DEĞERLENDİRİLMESİ
Authors: Yavaşoğlu Filiz , Özdemir Çiğdem , Köken Tülay
Published: April 2022

AMAÇ: İmmünofenotipleme, B hücre malignitesi teşhisi için kritik öneme sahiptir. Bu çalışmada klinik ve morfolojik olarak Kronik Lenfositik Lösemi (KLL) tanısı almış vakalarımızda akım sitometri yöntemi ile hücre yüzey antijen ekspresyon yüzdelerini ve yoğunluklarını değerlendirmeyi amaçladık.GEREÇ VE YÖNTEM: 33 KLL hastasının (12 kadın / 21 erkek) periferik venöz kanına ait akım sitometri sonuçları geriye dönük incelenmiştir. İlk olarak CD45-Side Scatter grafiği üzerinde lenfositler işaretlendi ve bu popülasyondaki antikor ekspresyon yüzdeleri ve yoğunlukları belirlendi.BULGULAR: Tüm hastalarda klasik paternde olduğu gibi CD5, CD19, CD20, CD23, CD200 ve CD43 antijen ekspresyonlarının mevcut olduğu görülmüştür. Klasik paternden farklı olarak ekspresyonu beklenmeyen FMC7’nin sekiz hastada ekspresyonu %30 un üzerindedir. Vakaların 6’sı kappa, 15’i lambda monoklonalitesi gösterirken 11 vakada da her iki hafif zincir ekspresyonunun %30’un altında olduğu görüldü. ZAP 70 ekspresyonu üç vakada (%16, %23, %42) rastlanmıştır. Tüm vakaların antijen ekspresyonlarının ortalama yüzdelerine bakıldığında CD5, CD19, Cd20, Cd23, CD200 ve CD43 ün %80’nin üzerinde olduğu görülmektedir.SONUÇ: KLL tanısında bakılması önerilen yüzey antijen ekspresyonları her vakada tanımlanan kurallara uymayabilir. Bu vakalarda tanı algoritmasına girecek daha spesifik ek hücre markırlarının araştırılmasına ihtiyaç ortaya çıkmaktadır.
Reversible facial nerve palsy associated with acute parotitis- A case report
Authors: Singh Harjitpal , Guleria Trilok Chand , Sharma Disha , Saini Amit , Ranot Bharti , Kaur Ravinder
Published: April 2022

Parotid infection/abscess leading to facial nerve palsy is a rare presentation. Normally, malignant parotid masses are associated with facial nerve palsy. We present a case of parotitis which was complicated by facial nerve dysfunction and recovered well with conservative treatment.
Medición del impacto socioeconómico del desarrollo de infraestructura de última milla en Jamaica
Authors: Puig Gabarró Pau , Katz Raúl , Galperin Hernán , Callorda Fernando , Iglesias Rodriguez Enrique , García Zaballos Antonio , Robles Marcos , Valencia Ramiro
Published: April 2022

El presente documento busca adentrarse en la medición del impacto económico y social del despliegue de infraestructura digital de última milla en Jamaica (por ejemplo, en el nivel de gasto de la población y los indicadores laborales). Acceder a esta información puede ayudar al gobierno de Jamaica a tomar decisiones de políticas públicas sobre la conveniencia de extender el despliegue de banda ancha y generar incentivos para la adopción de la conectividad en los hogares. Los resultados demuestran la importancia de acompañar el despliegue de banda ancha con políticas públicas que promuevan un beneficio equitativo de la tecnología. En este sentido, se destaca la necesidad de realizar acciones de alfabetización digital en la población con menor nivel educativo, así como implementar medidas específicas para reducir la brecha por género.
A nyelvjárási adatközlő nyelvi konstruálása : Esettanulmány a Szlovákiai Magyar Nyelvjárási Hangoskönyv szövegeiből
Authors: Tolcsvai Nagy Gábor , Presinszky Károly
Published: April 2022

A szerzők komplex módon, a korpusz egységeit szövegként értelmezve vizsgálják szlovákiai kisebbségi beszélők spontán nyelvjárási beszédeinek (interjúinak) jelentéstani és pragmatikai jellemzőit. A Szlovákiai Magyar Nyelvjárási Hangoskönyv rögzített, lejegyzett anyagából három nyelvjárási régióból kilenc szöveg (szövegrészlet) vizsgálatára kerül sor. A vizsgálat a következő négy szempont szerint történt: az adatközlők mondatszerkesztésének jellemzői (a kifejezett jelenet, típusa, a szereplők és körülmények megkonstruálásának jellemzői; a mondatban a beszélő által megvalósított közlési funkció; a kontextualizálók szerepe); az adatközlőtől megkérdezett központi fogalom szemantikai megkonstruálásának módja; az adatközlőnek a témához való viszonya és a szubjektivizáltság mértéke; az adatközlőnek a beszédhelyzethez való viszonya (az interjú beszédhelyzetében). Az eredmények azt jelzik, hogy a korpuszban a leggyakoribb az alapértelmezés szerinti (legegyszerűbb) mondattípus, a semleges pozitív kijelentő mondat. Ezeket korlátozott információmennyiség (csak a szükséges bővítmények és módosítók sora) jellemzi, mindennapi helyi eseményeket, állapotokat és helyzeteket kifejezve pozitív kijelentésekben, helyi közösségi, tér- és időbeli kontextuális elemekre utalva. A beszélgetések központi témáit a beszélők keretek és forgatókönyvek Gestaltként való szemantikai és pragmatikai használata révén fejtik ki, csak néhány jellegzetes elemre utalva. A beszélők az eseményeket és helyzeteket nagyfokú objektivizálással konstruálják meg, bár ezek a részek szubjektivizált nézőpontba vannak foglalva, főképp diskurzusjelölőkkel. A beszélők a beszédhelyzethez való viszonyukat általában impliciten diskurzusjelölőkkel és metapragmatikai kifejezésekkel jelzik, nem túl nagy számban. A három nyelvjárási régióból származó interjúszövegek között az elemzett adatok alapján nincsen lényeges különbség, miközben fonológiai és morfológiai tekintetben erősen eltérnek.