bluuebunny/crossref_metadata_2025 huggingface dataset:
Few-Shot Air Object Detection Network
Authors: Cai Wei , Wang Xin , Jiang Xinhao , Yang Zhiyong , Di Xingyu , Gao Weijie
Published: October 2023

Focusing on the problem of low detection precision caused by the few-shot and multi-scale characteristics of air objects, we propose a few-shot air object detection network (FADNet). We first use a transformer as the backbone network of the model and then build a multi-scale attention mechanism (MAM) to deeply fuse the W- and H-dimension features extracted from the channel dimension and the local and global features extracted from the spatial dimension with the object features to improve the network’s performance when detecting air objects. Second, the neck network is innovated based on the path aggregation network (PANet), resulting in an improved path aggregation network (IPANet). Our proposed network reduces the information lost during feature transfer by introducing a jump connection, utilizes sparse connection convolution, strengthens feature extraction abilities at all scales, and improves the discriminative properties of air object features at all scales. Finally, we propose a multi-scale regional proposal network (MRPN) that can establish multiple RPNs based on the scale types of the output features, utilizing adaptive convolutions to effectively extract object features at each scale and enhancing the ability to process multi-scale information. The experimental results showed that our proposed method exhibits good performance and generalization, especially in the 1-, 2-, 3-, 5-, and 10-shot experiments, with average accuracies of 33.2%, 36.8%, 43.3%, 47.2%, and 60.4%, respectively. The FADNet solves the problems posed by the few-shot characteristics and multi-scale characteristics of air objects, as well as improving the detection capabilities of the air object detection model.
A Fast Adaptive Binarization Method for QR Code Images Based on Dynamic Illumination Equalization
Authors: Chen Rongjun , Huang Yue , Lan Kailin , Li Jiawen , Ren Yongqi , Hu Xianglei , Wang Leijun , Zhao Huimin , Lu Xu
Published: October 2023

The advancement of Internet of Things (IoT) has enhanced the extensive usage of QR code images in various computer vision applications. Nonetheless, this has also brought forth several technical challenges. In particular, the logistics sorting system often encounters issues such as a low recognition rate and slow processing speed when dealing with QR code images under complex lighting conditions like uneven illumination. To address these difficulties, a method that focuses on achieving a fast adaptive binarization of QR code images through dynamic illumination equalization was proposed. First, an algorithm based on edge enhancement to obtain the position detection patterns within QR code images was applied, which enabled the acquisition of structural features in uneven illumination. Subsequently, QR code images with complex lighting conditions can achieve a fast adaptive binarization through dynamic illumination equalization. As for method validation, the experiments were performed on the two datasets that include QR code images influenced by strong light, weak light, and different shadow degrees. The results disclosed the benefits of the proposed method compared to the previous approaches; it produced superior recognition rates of 78.26–98.75% in various cases through commonly used decoders (Wechat and Zxing), with a faster processing speed of 0.0164 s/image, making it a proper method to satisfy real-time requirements in practical applications, such as a logistics sorting system.
Siamese Trackers Based on Deep Features for Visual Tracking
Authors: Lim Su-Chang , Huh Jun-Ho , Kim Jong-Chan
Published: October 2023

Visual object tracking poses challenges due to deformation of target object appearance, fast motion, brightness change, blocking due to obstacles, etc. In this paper, a Siamese network that is configured using a convolutional neural network is proposed to improve tracking accuracy and robustness. Object tracking accuracy is dependent on features that can well represent objects. Thus, we designed a convolutional neural network structure that can preserve feature information that is produced in the previous layer to extract spatial and semantic information. Features are extracted from the target object and search area using a Siamese network, and the extracted feature map is input into the region proposal network, where fast Fourier-transform convolution is applied. The feature map produces a probability score for the presence of an object region and an object in a region, where the similarities are high to search the target. The network was trained with a video dataset called ImageNet Large Scale Visual Recognition Challenge. In the experiment, quantitative and qualitative evaluations were conducted using the object-tracking benchmark dataset. The evaluation results indicated competitive results for some video attributes through various experiments. By conducting experiments, the proposed method achieved competitive results for some video attributes, with a success metric of 0.632 and a precision metric of 0.856 as quantitative values.
Market-Based TSO–DSO Coordination: A Comprehensive Theoretical Market Framework and Lessons from Real-World Implementations
Authors: Troncia Matteo , Chaves Ávila José Pablo , Damas Silva Carlos , Gerard Helena , Willeghems Gwen
Published: October 2023

This paper introduces a theoretical market framework (TMF) for conceptualizing and designing electricity markets, integrating transmission system operator and distribution system operator (TSO–DSO) coordination mechanisms. The TMF represents a comprehensive tool that formalizes new, innovative market concepts and their impact on existing markets, and outlines fundamental categories and decisions essential to market design. This paper, through the TMF, addresses the integration challenges posed by new mechanisms for system services. Utilizing the TMF, the study maps 13 European demonstrators’ TSO–DSO coordination solutions, identifying real-world challenges in designing and implementing novel system services markets. Drawing on these real-world insights, the paper offers market design and policy recommendations to address and overcome the specific challenges in market-based TSO–DSO coordination.
Ceramic Materials Containing Volcanic Ash and Characterized by Photoluminescent Activity
Authors: Candamano Sebastiano , De Luca Pierantonio , Garofalo Pietro , Crea Fortunato
Published: October 2023

The present work was aimed at investigating the possibility to produce bricks using volcanic ash from the Etna volcano and with photoluminescent properties. The volcanic ash was analyzed using X-ray diffractometry, scanning electron and energy dispersive X-ray microscopy. Mixtures with 0%, 10% and 30% of volcanic ash were prepared and fired at 950 °C for 14 h. Their mechanical properties, dynamic modulus of elasticity, drying linear retraction, capillary water absorption and water absorption were measured. The obtained results show that increasing the percentage of volcanic ash in the initial mixture results in a decrease in the measured dynamic modulus of elasticity, in a greater dimensional stability and in a lower weight loss upon firing. Mechanical properties are negatively affected by volcanic ash, with a compressive strength of 11 MPa obtained with mixtures with 30% of it. A decrease in the absorption of water was also observed upon increasing the amount of volcanic ash in the mixes. Brick surfaces with intense photoluminescent activity were obtained. The produced bricks meet the criteria required by the for bricks that can be used in normal weathering and absorption criteria for second-class brick.
Removal of Cadmium and Lead from Synthetic Wastewater Using Galdieria sulphuraria
Authors: Kharel Hari Lal , Shrestha Ina , Tan Melissa , Selvaratnam Thinesh
Published: October 2023

The strain of red microalgae Galdieria sulphuraria CCMEE 5587.1 was evaluated in a controlled laboratory environment for its ability to tolerate and remove two heavy metal (HM) ions: cadmium [Cd(II)] and lead [Pb(II)] in aqueous solutions as a single metal species. Various concentrations (0 mg L−1 to 5 mg L−1) of Cd and Pb ions were added to the Cyanidium medium in which the chosen microalgae strain G. sulphuraria CCMEE 5587.1 was grown at an acidic pH of 2.5. The effectiveness of G. sulphuraria CCMEE 5587.1 in tolerating and removing these two metal ions was measured by analyzing its growth profile, growth rate, nutrient removal, and metal ion removal efficiency. The growth of G. sulphuraria CCMEE 5587.1 was inhibited during the initial days of incubation, and the growth rate decreased when the HM concentration in the media was increased. Nutrient removal in the HM-containing media is comparable to that in the control media at low metal concentrations but decreases as the metal concentration rises. G. sulphuraria CCMEE 5587.1 has the highest removal efficiency for Cd and Pb in a medium containing 2.5 mg L−1 of metal ions, which is 49.80% and 25.10%, and the corresponding sorption capacity is 1.45 mg g−1 and 0.53 mg g−1 of dry biomass, respectively. These findings suggest that G. sulphuraria CCMEE 5587.1 holds potential as a viable bioremediation solution for extracting Cd and Pb from wastewater, alongside its capacity to remove nutrients concurrently. The study underscores the dual advantage of G. sulphuraria CCMEE 5587.1, making it a promising candidate for addressing heavy metal pollution in wastewater treatment processes.
Differential Sorption of Short-Chain versus Long-Chain Anionic Per- and Poly-Fluoroalkyl Substances by Soils
Authors: Brusseau Mark L.
Published: October 2023

The impact of chain length on the sorption of anionic PFAS by soils and sediments was investigated by aggregating and synthesizing data sets from the literature. Quantitative structure/property relationship (QSPR) analysis was applied to characterize the influence of molecular size and soil properties on sorption. The log of the organic carbon-normalized equilibrium sorption coefficient (Koc) exhibited a biphasic relationship with molar volume, wherein the log Koc values for the short-chain PFAS were generally greater than would be predicted using the QSPR correlation determined for the long-chain PFAS. This enhanced differential sorption is observed to different degrees for all studies, which are compiled and synthesized for the first time. The results reveal remarkable congruency across a wide array of soils comprising a large range of properties and indicate that the observed enhanced differential sorption of short-chain PFAS is a prevalent phenomenon. Aggregating the long-chain PFAS data for all soils and sediments with organic carbon contents > 1% produced a strong correlation, indicating that the resultant QSPR model can produce representative log Koc values irrespective of the other properties of the medium. Silt+clay content was shown to be an important soil component for the short-chain PFAS for most soils, as well as the long-chain PFAS for soils with organic carbon contents < 1%. The results indicate that while the simple Koc-foc approach may produce reasonable estimates of Kd values for long-chain anionic PFAS, particularly for soils and sediments with larger organic carbon contents, it is unlikely to do so for short-chain anionic PFAS.
Evaluating MPTCP Congestion Control Algorithms: Implications for Streaming in Open Internet
Authors: Łuczak Łukasz Piotr , Ignaciuk Przemysław , Morawski Michał
Published: October 2023

In today’s digital era, the demand for uninterrupted and efficient data streaming is paramount across various sectors, from entertainment to industrial automation. While the traditional single-path solutions often fell short in ensuring rapid and consistent data transfers, Multipath TCP (MPTCP) emerges as a promising alternative, enabling simultaneous data transfer across multiple network paths. The efficacy of MPTCP, however, hinges on the choice of appropriate congestion control (CC) algorithms. Addressing the present knowledge gap, this research provides a thorough evaluation of key MPTCP CC algorithms in the context of streaming applications in open Internet environments. Our findings reveal that BALIA stands out as the most suitable choice for MPTCP streaming, adeptly balancing waiting time, throughput, and Head-of-Line blocking reduction. Conversely, the wVegas algorithm, with its delay-centric approach, proves less adequate for multipath streaming. This study underscores the imperative to fine-tune MPTCP for streaming applications, at the same time offering insights for future development areas and innovations.
Optimization and Investigation of Rapid Sealing Technology Based on Mine Disaster Period
Authors: Li Feng , Jing Yadong , Wang Chenchen , Ren Baorui , Zhang Chenyu , Wang Guanghao
Published: October 2023

Due to the particularity of mine spaces and the limitations of underground ventilation, the gas in disaster areas changes greatly after a fire occurs. Rapid sealing technology is beneficial for preventing the development of fires and gas explosions by controlling oxygen. Using the Fuzzy Analytic Hierarchy Process (FAHP), in this research, we analyzed the three most effective rapid sealing processes, conducted experimental research on the three sealing processes, and developed a further optimized design. At the same time, according to different stages of blasting damage, the change characteristics and migration laws of explosive hazardous gases in a disaster area were analyzed using fluent numerical simulation. Additionally, the ability of the three optimal processes to create an airtight area was measured in this research. The applicable scenarios and scope of the three technologies were found, which provides a wider range of application scenarios and more diverse options for rapid airtightness during catastrophic periods, mine fire prevention, and explosion protection.
Environmental DNA Analysis in a River Detected a Possible Distribution of Fish Species Difficult to Capture
Authors: Nakamichi Tomoki , Ono Masahiro , Hayashi Masatoshi , Okamura Takahiko , Wada Toshihiro , Saitoh Kenji
Published: October 2023

Environmental DNA (eDNA) analysis is a biological survey method that has drawn much attention in recent years. However, the results of eDNA analysis and capture surveys often do not completely match, and the validity of the eDNA analysis needs to be verified. Verification of eDNA metabarcoding was conducted in a river in Fukushima Prefecture, Japan, in comparison with capture survey data. Most of the captured species were detected, and 13 uncaptured lineages (two genera and 11 species) were detected in the eDNAs. Some rare species detected in the eDNAs were also identified, including exotic eels and an endangered yet out-of-range bitterling fish. To confirm the validity of the exotic Anguilla spp. detected in eDNAs, mitochondrial Cytb sequencing was performed on captured eel specimens. All eel specimens were sequenced as the native Anguilla japonica, indicating a small biomass of the exotic species difficult to capture. Our results clearly indicated the eDNA analysis as a powerful tool for detecting possible habitats of rare fish species without disturbance to the natural environment.
Unveiling the Potential of Cavitation Erosion-Induced Heavy Crude Oil Upgrading
Authors: Baimukhametov Gadel F. , Kayumov Aydar A. , Dengaev Aleksey V. , Maksimenko Alexander F. , Marakov Denis A. , Shishulin Vladimir A. , Drozdov Ilya M. , Samuylova Larisa V. , Getalov Andrey A. , Aliev Firdavs A. , Vakhin Alexey V.
Published: October 2023

The application of ultrasonic waves in the processing of hydrocarbons is a new promising technology that has developed rapidly in recent years. However, the acoustic-induced cavitation erosion phenomenon is poorly studied. In this paper, a comparison study of cavitation erosion was carried out in water and oils with different viscosities produced from Ashal’cha and North Komsomol using an ultrasonic reactor operating at an industrial frequency of 20 kHz. The acoustic spectra obtained from hydrophones during the ultrasonic treatment of fluids can be characterized by using subharmonics of the main frequency and a continuous white noise level. Moreover, the cavitation erosion of aluminum foil under various ultrasound irradiation times and power levels was thoroughly investigated. It has been found that the process of ultrasonic cavitation has a less erosive impact on metal foil in oil due to its high viscosity. In addition, the formation of microflows in the oil phase, which also intensify the erosion process, is hindered. Cavitation erosion in the Ashal’cha oil sample exhibited a higher intensity compared to that in the North Komsomol oil sample. It was found that upon increasing ultrasound intensity in the case of the viscous (Ashal’cha) oil sample, cavitation stability was disrupted. In turn, this led to a reduction in the collapse energy of the cavitation bubbles. The results we obtained enable the assessment of cavitation erosion in crude oil and could be used to improve methodologies for monitoring and optimizing cavitation processes in crude oil.
Assessment of the Quality, Chemometric and Pollen Diversity of Apis mellifera Honey from Different Seasonal Harvests
Authors: Rivera-Mondragón Andrés , Marrone Maravi , Bruner-Montero Gaspar , Gaitán Katerin , de Núñez Leticia , Otero-Palacio Rolando , Añino Yostin , Wcislo William T. , Martínez-Luis Sergio , Fernández-Marín Hermógenes
Published: October 2023

The parameters for assessing the quality of honey produced by Apis mellifera are standardized worldwide. The physicochemical properties of honey might vary extensively due to factors such as the geographical area where it was produced and the season in which it was harvested. Little information is available on variations in honey quality among different harvest periods in tropical areas, and particularly in neotropical dry forests. This study describes variations in seventeen physicochemical parameters and the pollen diversity of honey harvested from beehives during the dry season in February, March, and April 2021, in the dry arc of Panama. Potassium is the most abundant mineral in honey samples, and its concentration increases during the harvest period from February to April. A PCA analysis showed significant differences among the samples collected during different harvest periods. The pollen diversity also differs among honey samples from February compared with March and April. The results indicate that climatic conditions may play an important role in the quality of honey produced in the dry arc of Panama. Furthermore, these results might be useful for establishing quality-control parameters of bee honey produced in Panama in support of beekeeping activities in seasonal wet–dry areas of the tropics.
Effects of Leaf Removal on Free and Glycoconjugate Aromas of Skins and Pulps of Two Italian Red Grapevine Varieties
Authors: Piombino Paola , Pittari Elisabetta , Genovese Alessandro , Bellincontro Andrea , Failla Osvaldo , Moio Luigi
Published: October 2023

Leaf removal is a cultural practice mainly aimed at improving cluster zone microclimates and impacting primary and secondary metabolites, such as volatiles. This research aimed to assess the impact of defoliation on free and glycosylated aromas of a neutral (‘Nebbiolo’) and a semi-aromatic (‘Aleatico’) red variety. Defoliation was performed at fruit set (BBCH 71) and, for ‘Nebbiolo’, also at berries touch (BBCH 81) phenological stages. Skins and pulps were separately analyzed by Solid Phase Extraction–Gas Chromatography/Mass Spectrometry. Results showed that the response to defoliation was variety-dependent. For ‘Nebbiolo’, especially when performed at the berries’ touch stage, defoliation had a significant effect on the accumulation of free volatiles and glycosidic precursors. Differently, free and bound ‘Aleatico’ volatiles were less impacted by defoliation. Interestingly, in both grapevine varieties, defoliation significantly enhanced the accumulation of aroma precursors in grapes’ skins, which is of particular relevance for red wine production and their aging potential. Moreover, results could be helpful for the management of grape quality, as defoliation is currently considered as a strategy to address climate change issues.
Mixed Multi-Chaos Quantum Image Encryption Scheme Based on Quantum Cellular Automata (QCA)
Authors: Mohamed Nehal Abd El-Salam , El-Sayed Hala , Youssif Aliaa
Published: October 2023

The advent of quantum computers could enable the resolution of complex computational problems that conventional cryptographic protocols find challenging. As a result, the formidable computing capabilities of quantum computers may render all present-day cryptographic schemes that rely on computational complexity ineffectual. Inspired by these possibilities, the primary purpose of this paper is to suggest a quantum image encryption scheme based on quantum cellular automata with mixed multi-chaos hybrid maps and a hyperchaotic system with quantum operations. To achieve desirable encryption outcomes, we designed an encryption scheme involving two main operations: (1) pixel-level diffusion and (2) pixel-level permutation. Initially, the secret keys generated using the hyperchaotic system were closely tied to the original image. During the first phase, the establishment of correlations among the image pixels, in addition to the three chaotic sequences obtained from the hyperchaotic system, was achieved with the application of a quantum-state superposition and measurement principle, wherein the color information of a pixel is described using a single qubit. Therefore, the three channels of the plain image were subjected to quantum operations, which involve Hadamard transformation and the quantum-controlled NOT gate, before the diffusion of each color channel with the hyperchaotic system. Subsequently, a quantum ternary Toffoli gate was used to perform the diffusion operation. Next, the appropriate measurement was performed on the three diffused channels. To attain the confusion phase, a blend of mixed multi-chaos hybrid maps and a two-dimensional quantum cellular automaton was used to produce random and chaotic sequence keys. Subsequently, the circular shift was utilized to additionally shuffle the rows and columns of the three diffused components, in order to alter the positions of their pixel values, which significantly contributes to the permutation process. Lastly, the three encoding channels, R, G, and B, were merged to acquire the encrypted image. The experimental findings and security analyses established that the designed quantum image encryption scheme possesses excellent encryption efficiency, a high degree of security, and the ability to effectively withstand a diverse variety of statistical attacks.
Edible Gels with Cranberry Extract: Evaluation of Anthocyanin Release Kinetics
Authors: Šedbarė Rima , Janulis Valdimaras , Ramanauskiene Kristina
Published: October 2023

The bioactive compounds found in cranberry fruit are natural antioxidants, and their consumption reduces the risk of diabetes, cardiovascular disease, cancers, and urinary tract infections. Oral gels with cranberry fruit extract are a promising product that can ensure accurate dosage and release of the active compounds and are suitable for people with dysphagia. The aim of this study was to determine the effect of polymeric materials on the dissolution kinetics of cranberry fruit anthocyanins from gel formulations. Gel formulations were prepared using freeze-dried cranberry fruit extract with different gelling excipients: chitosan (G1–G3), sodium carboxymethylcellulose (G4–G6), and sodium carboxymethylcellulose combined with carbomers (G7–G9). The dissolution test showed that the release of anthocyanins from gel formulations G1–G6 and G9 was most intense within the first 10 min, with little change in the anthocyanin content of the acceptor medium afterwards. For the formulations based on carboxymethyl cellulose and carbomers G7 and G8, the amount of anthocyanins released into the acceptor medium gradually increased, which prolonged the release time of the active compounds. The test for the release of anthocyanins from the semi-solid systems through a hydrophilic membrane revealed that within the first hour, the total amount of anthocyanins released from the modeled gel formulations (G1–G9) was within the range of 6.02%–13.50%. The 1% chitosan (G1) gel formulation released the fastest and highest amount of anthocyanins (70% within 6 h). The other formulations showed a slower release of anthocyanins, and after 6 h, the amount of anthocyanins released from formulations G2–G9 was <57%.
Colorimetric Sensors Based on Poly(acrylic Acid)/TiO2 Nanocomposite Hydrogels for Monitoring UV Radiation Exposure
Authors: Botti Sabina , Bonfigli Francesca , D’Amato Rosaria , Rodesi Jasmine , Santonicola Maria Gabriella
Published: October 2023

In recent years, there has been an open debate on proper sun exposure to reduce the risk of developing skin cancer. The mainly encountered issue is that general guidelines for UV radiation exposure could not be effective for all skin types. The implementation of customized guidelines requires a method by which to measure the UV dose as a result of daily exposure to sunlight, ideally with an inexpensive, easy-to-read sensor. In this work, we present the characterization of nanocomposite hydrogel materials acting as colorimetric sensors upon exposure to UV light. The sensor was prepared using a poly(acrylic acid) (PAA) hydrogel matrix in which TiO2 nanoparticles and methylene blue (MB) were integrated. Raman mapping was used to determine the network structure of the hydrogel and its water distribution. The TiO2 nanoparticles dispersed in the PAA matrix maintain their photoactivity and catalyze a reaction by which methylene blue is converted into leuko-methylene. The conversion causes a discoloration effect that is visible to the naked eye and can therefore be used as an indicator of UV radiation exposure. Moreover, it was possible to tune the discoloration rate to the limit exposure of each skin type, simply by changing the ratio of titanium dioxide to dye. We obtained a response time ranging from 30 min to 1.5 h. Future work will be dedicated to the possibility of scaling up this range and to improve the sensor wearability; however, our study paves the way to the realisation of sensors suitable for public use, which could help us find a solution to the challenge of balancing sufficient UV exposure to prevent Vitamin D deficiency with excessive UV exposure that could ultimately cause skin cancer.
Is Sea Level Rise a Known Threat? A Discussion Based on an Online Survey
Authors: Solarino Stefano , Eva Elena , Anzidei Marco , Musacchio Gemma , De Lucia Maddalena
Published: October 2023

Since the last century, global warming has been triggering sea level rise at an unprecedented rate. In the worst case climate scenario, sea level could rise by up to 1.1 m above the current level, causing coastal inundation and cascading effects, thus affecting about one billion people around the world. Though widespread and threatening, the phenomenon is not well known to citizens as it is often overshadowed by other effects of global warming. Here, we show the results of an online survey carried out in 2020–2021 to understand the level of citizens’ knowledge on sea level rise including causes, effects, exacerbation in response to land subsidence and best practice towards mitigation and adaptation. The most important result of the survey is that citizens believe that it is up to governments to take action to cope with the effects of rising sea levels or mitigate the rise itself. This occurs despite the survey showing that they actually know what individuals can do and that a failure to act poses a threat to society. Gaps and preconceptions need to be eradicated by strengthening the collaboration between scientists and schools to improve knowledge, empowering our society.
Advances in Postharvest Diseases Management of Fruits and Vegetables: A Review
Authors: Moradinezhad Farid , Ranjbar Azam
Published: October 2023

Sustainable agriculture requires factors to directly stimulate plant growth and induce the plant’s innate immune system to protect against stresses. Protection of plants is one of the main approaches to the supply of food resource. Furthermore, improved techniques for plant disease management must be environmentally sustainable, reliable, acceptable by society, and chemical-free to ensure sustainable food security. Although it is not possible to accurately determine postharvest losses due to diseases and physiological disorders, the use of proper harvesting and transportation methods that minimize damage to the product, along with optimal storage conditions that prevent the development of diseases, will be effective in reducing these postharvest losses. Since handling and storage conditions are potential threats for postharvest spoilage, it is necessary to identify environmentally friendly approaches and their precision mechanisms for postharvest disease management. Recently, biological control, non-chemical, and eco-friendly techniques have been investigated for this purpose.
Effect of Preharvest Ethephon Application on Selected Biochemical Components and Polyphenol Oxidase Activity in Macadamia Nuts
Authors: Aruwajoye Noluthando Noxolo , Mditshwa Asanda , Magwaza Lembe Samukelo , Ngidi Mjabuliseni Simon Cloapas , Tesfay Samson Zeray
Published: October 2023

Ethephon is a plant growth regulator that triggers diverse responses in plants, such as fruit ripening, leaf senescence, hull senescence, stem elongation, and nut abscission. This study examined how the preharvest application of ethephon 480 SL® affects selected biochemical components and polyphenol oxidase (PPO) activity in two macadamia nut cultivars: ‘788’ and ‘Beaumont’. Ethephon was applied to the trees via a Cima mist blower at rates of 13.33 mL/L per hectare for the ‘788’ cultivar and 16.67 mL/L per hectare for the ‘Beaumont’. Following harvest, the nuts were stored at 25 °C for 72 days, and samples were taken at 18-day intervals. Standard procedures were used to assess the following: total phenolics, total flavonoids, 2,2,-diphenyl-1-picrylhydrazyl (DPPH) assay, Ferric reducing ability power (FRAP) assay, sucrose, total protein, and PPO activities. This evaluation was carried out across a total of four treatments: ethephon-treated nuts from the orchard floor (ED), ethephon-treated nuts from the tree (ET), untreated nuts from the orchard floor (CD), and untreated nuts from the tree (CT). The evaluation’s outcomes were analyzed using a principal component analysis (PCA), a correlation matrix heat map (CMHM), and a graphical assessment. The results unveiled significant correlations and associations among the assessed parameters. The correlation matrix heat map analysis highlighted a strong positive correlation (0.97) between the sucrose and the PPO activity in the ‘Beaumont’ cultivar, supported by the PCA analysis identifying the ED treatment as the most influential. At the storage period’s conclusion, the ED treatment had the highest sucrose content (18.63 mg/g) and polyphenol oxidase activity (1.06 U g−1). In the ‘788’ cultivar, a close relationship emerged between the phenolic content, the PPO activity, and the Ferric reducing antioxidant power (FRAP)’s antioxidant activity. Consistently, the CT treatment (untreated nuts) demonstrated positive correlations with several key parameters in both cultivars, displaying heightened phenolic content and antioxidant activities. Consequently, our findings indicate that the CT treatment, involving tree-harvested nuts without ethephon application, could be the preferred option for sustaining macadamia nuts’ quality and shelf life compared to other methods. Moreover, our study underscores the significance of proper storage conditions for maintaining the desired biochemical parameters of macadamia nuts. By comprehending the effects of distinct treatments and harvesting techniques, producers and processors can devise strategies to optimize storage conditions and uphold macadamia nut quality.
Growth Responses and Adventitious Root Formation of Cucumber Hybrid Lines in a Waterlogged Condition
Authors: Kolozs Henriett , Szegő Anita , Kiss-Bába Erzsébet , Hesari Neda , Cardoso Juliana Teles , Mirmazloum Iman , Papp István
Published: October 2023

Cucumber (Cucumis sativus L.) F1 hybrids are grown commercially in open-field or greenhouse conditions. Hybrids are well adapted to these settings due to directed breeding. In protected cultivation systems, a small rhizosphere volume and intensive, continuous fertigation predispose the roots to waterlogging (WL) conditions and potentially to hypoxia. However, high productivity is expected and achieved under these conditions. The aim of this study was to identify traits that play a role in this surprising behavior. Initial observations revealed the presence of a significantly higher number of adventitious roots (ARs) in three greenhouse (7–14) vs. three open-field cultivars (less than two) grown under normal conditions. Further on, two contrasting representative hybrids typically grown in open-field and in greenhouse conditions were subjected to WL stress. Declining oxygen levels in the media and increased alcohol dehydrogenase activity (ADH) in the roots were experienced during the WL treatment in both hybrids, with anaerobic metabolism triggered less intensively (~4-fold less ADH activity) in the greenhouse-type ‘Oitol’. The induction characteristics of cysteine oxidase (CysOx) genes, key components of the hypoxia sensing pathway, were used to confirm the hypoxic stress experienced by the roots. The lower extent of upregulation in CysOx genes expression agreed with the milder level of hypoxic stress in the roots of ‘Oitol’ than in ‘Joker’. The more efficient induction of AR formation with a ~50% increase upon waterlogging stress was found to be a prominent trait in ‘Oitol’, apparently helping root internal aeration and mitigating hypoxia. The shoot growth of neither hybrid was set back by hypoxic root conditions. ‘Joker’ plants maintained the same growth rate as that of the control, while the growth of ‘Oitol’ accelerated when its root system was flooded with nutrient solution. Acclimation processes to hypoxia were proposed to explain the lack of growth retardation in both varieties. This corresponded well with a general abundance of AR development in greenhouse-type (slicing) cucumbers that are typically cultivated in soilless systems.
Experiences Pertaining to Successful Aging in Middle-Aged Women in South Korea
Authors: Lee Do-young , Kim Hyun-ju , Jo A-young
Published: October 2023

This study aimed to analyze and gain an in-depth understanding of the experiences pertaining to successful aging in middle-aged women in South Korea. A sample of 12 middle-aged women, capable of sharing their lived experiences, was divided into three age-based groups: those in their 40s, those in their 50s, and those aged 60–65 years. The collected data were analyzed using Colaizzi’s phenomenological method. Five theme clusters and ten themes emerged. The experiences of successful aging among middle-aged women were categorized as: “Coping with changes in the mind and body”, “Financially stable life”, “Undergoing the aging process with a healthy family”, “Preparations for dying well”, and “Pursuing a meaningful, harmonious life”. These findings highlight the need for programs that prepare middle-aged women to positively accept and enjoy older adulthood by identifying and addressing the factors essential for successful aging and reducing any negative emotions attached to aging and older adulthood.
INT-767—A Dual Farnesoid-X Receptor (FXR) and Takeda G Protein-Coupled Receptor-5 (TGR5) Agonist Improves Survival in Rats and Attenuates Intestinal Ischemia Reperfusion Injury
Authors: Canovai Emilio , Farré Ricard , Accarie Alison , Lauriola Mara , De Hertogh Gert , Vanuytsel Tim , Pirenne Jacques , Ceulemans Laurens J.
Published: October 2023

Intestinal ischemia is a potentially catastrophic emergency, with a high rate of morbidity and mortality. Currently, no specific pharmacological treatments are available. Previous work demonstrated that pre-treatment with obeticholic acid (OCA) protected against ischemia reperfusion injury (IRI). Recently, a more potent and water-soluble version has been synthesized: Intercept 767 (INT-767). The aim of this study was to investigate if intravenous treatment with INT-767 can improve outcomes after IRI. In a validated rat model of IRI (60 min ischemia + 60 min reperfusion), three groups were investigated (n = 6/group): (i) sham: surgery without ischemia; (ii) IRI + vehicle; and (iii) IRI + INT-767. The vehicle (0.9% NaCl) or INT-767 (10 mg/kg) were administered intravenously 15 min after start of ischemia. Endpoints were 7-day survival, serum injury markers (L-lactate and I-FABP), histology (Park–Chiu and villus length), permeability (transepithelial electrical resistance and endotoxin translocation), and cytokine expression. Untreated, IRI was uniformly lethal by provoking severe inflammation and structural damage, leading to translocation and sepsis. INT-767 treatment significantly improved survival by reducing inflammation and preserving intestinal structural integrity. This study demonstrates that treatment with INT-767 15 min after onset of intestinal ischemia significantly decreases IRI and improves survival. The ability to administer INT-767 intravenously greatly enhances its clinical potential.
miR-136 Regulates the Proliferation and Adipogenic Differentiation of Adipose-Derived Stromal Vascular Fractions by Targeting HSD17B12
Authors: Liu Jianhua , Che Yutong , Cai Ke , Zhao Bishi , Qiao Liying , Pan Yangyang , Yang Kaijie , Liu Wenzhong
Published: October 2023

Fat deposition involves the continuous differentiation of adipocytes and lipid accumulation. Studies have shown that microRNA miR-136 and 17β-hydroxysteroid dehydrogenase type 12 (HSD17B12) play important roles in lipid accumulation. However, the regulatory mechanism through which miR-136 targets HSD17B12 during ovine adipogenesis remains unclear. This study aimed to elucidate the role of miR-136 and HSD17B12 in adipogenesis and their relationship in ovine adipose-derived stromal vascular fractions (SVFs). The target relationship between miR-136 and HSD17B12 was predicted and confirmed using bioinformatics and a dual-luciferase reporter assay. The results showed that miR-136 promoted proliferation and inhibited adipogenic differentiation of ovine SVFs. We also found that HSD17B12 inhibited proliferation and promoted adipogenic differentiation of ovine SVFs. Collectively, our results indicate that miR-136 facilitates proliferation and attenuates adipogenic differentiation of ovine SVFs by targeting HSD17B12. These findings provide a theoretical foundation for further elucidation of the regulatory mechanisms of lipid deposition in sheep.
Crystal Structure of DNA Replication Protein SsbA Complexed with the Anticancer Drug 5-Fluorouracil
Authors: Su Hsin-Hui , Huang Yen-Hua , Lien Yi , Yang Po-Chun , Huang Cheng-Yang
Published: October 2023

Single-stranded DNA-binding proteins (SSBs) play a crucial role in DNA metabolism by binding and stabilizing single-stranded DNA (ssDNA) intermediates. Through their multifaceted roles in DNA replication, recombination, repair, replication restart, and other cellular processes, SSB emerges as a central player in maintaining genomic integrity. These attributes collectively position SSBs as essential guardians of genomic integrity, establishing interactions with an array of distinct proteins. Unlike Escherichia coli, which contains only one type of SSB, some bacteria have two paralogous SSBs, referred to as SsbA and SsbB. In this study, we identified Staphylococcus aureus SsbA (SaSsbA) as a fresh addition to the roster of the anticancer drug 5-fluorouracil (5-FU) binding proteins, thereby expanding the ambit of the 5-FU interactome to encompass this DNA replication protein. To investigate the binding mode, we solved the complexed crystal structure with 5-FU at 2.3 Å (PDB ID 7YM1). The structure of glycerol-bound SaSsbA was also determined at 1.8 Å (PDB ID 8GW5). The interaction between 5-FU and SaSsbA was found to involve R18, P21, V52, F54, Q78, R80, E94, and V96. Based on the collective results from mutational and structural analyses, it became evident that SaSsbA’s mode of binding with 5-FU diverges from that of SaSsbB. This complexed structure also holds the potential to furnish valuable comprehension regarding how 5-FU might bind to and impede analogous proteins in humans, particularly within cancer-related signaling pathways. Leveraging the information furnished by the glycerol and 5-FU binding sites, the complexed structures of SaSsbA bring to the forefront the potential viability of several interactive residues as potential targets for therapeutic interventions aimed at curtailing SaSsbA activity. Acknowledging the capacity of microbiota to influence the host’s response to 5-FU, there emerges a pressing need for further research to revisit the roles that bacterial and human SSBs play in the realm of anticancer therapy.
Evaluating an Immersive Virtual Classroom as an Augmented Reality Platform in Synchronous Remote Learning
Authors: Flórez Marulanda Juan Fernando , Collazos Cesar A. , Hurtado Julio Ariel
Published: October 2023

Previous research has explored different models of synchronous remote learning environments supported by videoconferencing and virtual reality platforms. However, few studies have evaluated the preference and acceptance of synchronous remote learning in a course streamed in an immersive or augmented reality platform. This case study uses ANOVA analysis to examine engineering students’ preferences for receiving instruction during the COVID-19 pandemic in three classroom types: face-to-face, conventional virtual (mediated by videoconferencing) and an immersive virtual classroom (IVC). Likewise, structural equation modeling was used to analyze the acceptance of the IVC perceived by students, this includes four latent factors: ease of receiving a class, perceived usefulness, attitude towards IVC and IVC use. The findings showed that the IVC used in synchronous remote learning has a similar level of preference to the face-to-face classroom and a higher level than the conventional virtual one. Despite the high preference for receiving remote instruction in IVC, aspects such as audio delays that affect interaction still need to be resolved. On the other hand, a key aspect for a good performance of these environments is the dynamics associated with the teaching–learning processes and the instructor’ qualities.
Biosecurity and Management Strategies for Economically Important Exotic Tephritid Fruit Fly Species in Australia
Authors: Hoskins Jessica L. , Rempoulakis Polychronis , Stevens Mark M. , Dominiak Bernard C.
Published: October 2023

Exotic tephritid incursions are of high concern to Australia’s biosecurity and its horticultural industries. It is vital that Australia remains ready to respond to incursions as they arise, as an incursion of tephritid fruit fly species will result in significant economic losses. In this review, we compared Australian incursion management strategies for fruit flies with global management strategies and identified possible areas where improvements could be made in an Australian context. Overall, Australia has a good understanding of the main tephritid threats, of which Bactrocera species from across the Torres Strait (northern Australia) are of most concern. Effective tools for tephritid detection and early warning surveillance at points of entry are in place at ports and in horticultural areas Australia-wide and provide the basis for initiating biosecurity responses in the event of an incursion. Area-wide control measures used in successful eradication attempts globally are available for use in Australia. However, a specific tephritid emergency response plan identifying suitable response measures and control options for species of concern is not yet available. We have identified that Australia has the policies and management tools available to respond to an exotic tephritid incursion, but the speed at which this could be accomplished would be greatly improved by the development of species-specific emergency response plans.
Detection of Early Myocardial Dysfunction by Imaging Biomarkers in Cancer Patients Undergoing Photon Beam vs. Proton Beam Radiotherapy: A Prospective Study
Authors: Abbasi Muhannad Aboud , Bruno Giulia , Di Stefano Cristina , Garcia Bello Laura , Laack Nadia N. , Corbin Kimberly S. , Whitaker Thomas J. , Pellikka Patricia A. , Mutter Robert W. , Villarraga Hector R.
Published: October 2023

1. Background: We sought to determine acute and subacute changes in cardiac function after proton beam (PBT) and photon beam (PhT) radiotherapy (RT) using conventional and two-dimensional speckle tracking echocardiography (2D-STE) in patients with malignant breast and thoracic tumors. 2. Methods: Between March 2016 and March 2017, 70 patients with breast or thoracic cancer were prospectively enrolled and underwent transthoracic echocardiography with comprehensive strain analysis at pretreatment, mid-treatment, end of treatment, and 3 months after RT. 3. Results: PBT was used to treat 44 patients; PhT 26 patients. Mean ± SD age was 55 ± 12 years; most patients (93%) were women. The median (interquartile range) of the mean heart dose was lower in the PBT than the PhT group (47 [27–79] vs. 217 [120–596] cGy, respectively; p < 0.001). Ejection fraction did not change in either group. Only the PhT group had reduced systolic tissue Doppler velocities at 3 months. 2D-STE showed changes in endocardial and epicardial longitudinal, radial, and circumferential early diastolic strain rate (SRe) in patients undergoing PhT (global longitudinal SRe, pretreatment vs. end of treatment (p = 0.04); global circumferential SRe, pretreatment vs. at 3-month follow-up (p = 0.003); global radial SRe, pretreatment vs. at 3-month follow-up (p = 0.02) for endocardial values). Epicardial strain values decreased significantly only in patients treated with PhT. Patients in the PhT group had a significant decrease in epicardial global longitudinal systolic strain rate (GLSRs) (epicardial GLSRs, at baseline vs. at end of treatment [p = 0.009]) and in GCSRe and GRSRe (epicardial GCSRe, at baseline vs. at 3-month follow-up (p = 0.02); epicardial GRSRe, at baseline vs. at 3-month follow-up (p = 0.03)) during treatment and follow-up. No changes on 2D-STE were detected in the PBT group. 4. Conclusions: Patients who underwent PhT but not PBT had reduced tissue Doppler velocities and SRe values during follow-up, suggesting early myocardial relaxation abnormalities. PBT shows promise as a cardiac-sparing RT technology.
Evaluation of Tropicamide–Phenylephrine Mydriatic Eye Drop Instillation on Choroidal Thickness
Authors: Gioia Marco , De Bernardo Maddalena , Pagliarulo Sergio , Cione Ferdinando , Mottola Francesco Ferdinando , La Marca Aniello , De Pascale Ilaria , Albano Giovanni , Rosa Nicola
Published: October 2023

The purpose of this study is to evaluate choroidal thickness (ChT) at the subfoveal and peripheral level after the instillation of 0.5% tropicamide + 10% phenylephrine 9 hydrochloride eye drops by using OCT scans in enhanced depth image (EDI) mode. In total, 53 patients (30 males and 23 females) were involved, and the mean age was 25.62 ± 2.41 (age range: 23–36). The dominant eye was treated with tropicamide + phenylephrine (Visumidriatic Fenil 100 mg/mL + 5 mg/mL, Visufarma) while the nondominant eye was used as the control. An OCT analysis was performed on both eyes before and 30 min after the instillation of a drop of mydriatic in the dominant eye. The ChT was measured by using the OCT software measurement tool (Spectralis; Heidelberg Engineering; Heidelberg, Germany, version 6.0). The results showed a statistically significant ChT decrease (p = 0.009) in the temporal sector after the treatment with tropicamide + phenylephrine. In the subfoveal and nasal sectors, no statistically significant ChT changes were detected (p = 0.94; p = 0.85) following the administration of the mydriatic eye drops. The ChT thinning in the temporal sector following the instillation of the tropicamide + phenylephrine eye drops suggests that in the case of ChT studies, mydriatic administration should be avoided.