bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of bioceramic root canal sealers on the bond strength of fiber posts cemented with resin cements
Authors: Nesello Rafael , Silva Isadora Ames , Bem Igor Abreu De , Bischoff Karolina , Souza Matheus Albino , Só Marcus Vinícius Reis , Rosa Ricardo Abreu Da
Published: April 2022

Abstract This study aimed to evaluate the influence of calcium silicate-based sealers on the bond strength of fiber posts using conventional and self-adhesive resin cement. Sixty single-rooted teeth were selected. The canals were prepared with a reciprocating instrument 40.06. The roots were randomly distributed in six groups (n = 10) according to the strategies for root canal filling and fiber posts cementation: AH Plus/RelyX ARC; AH Plus/RelyX U200; Bio C Sealer/RelyX ARC; Bio C Sealer/RealyX U200; Sealer Plus BC/RelyX ARC; and Sealer Plus BC/RelyX U200. The roots were transversally sectioned, and one slice per post-third was obtained. The push-out test was performed at a crosshead speed of 1mm/min. The failure patterns were described after assessment with a stereomicroscope with a 10× magnification. Bond strength was calculated and analyzed using the ANOVA and Tukey test. AH Plus did not influence the bond strength of fiber posts cemented with conventional (RelyX ARC) or with self-adhesive resin cement (RelyX U200). The lowest bond strength values were obtained when calcium silicate-based sealers were associated with conventional resin cement (Bio C Sealer/RelyX ARC and Sealer Plus BC/RelyX ARC). Except for Sealer Plus BC/RelyX ARC, all groups presented lower bond strength at the apical portion compared to the cervical portion of the post. Adhesive failures between cement and post and cement and dentin were predominant (55.3%). Calcium silicate-based sealers decreased the bond strength of fiber posts cemented with conventional resin cement.
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
Authors: Guimarães Taísa Coelho , Marques Barbara Bruno Fagundes , Tinoco Justine Monteiro Monnerat , Porto Luís Cristóvão Moraes Sobrino , Tinoco Eduardo Muniz Barretto , Fischer Ricardo Guimarães
Published: April 2022

Abstract The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs.
Shaping Ability of Reciprocating and Rotary Systems After Root Canal Retreatment: a CBCT Study
Authors: Suzuki Eduardo Hideki , Sponchiado-Júnior Emílio Carlos , Pandolfo Mariana Travi , Garcia Lucas da Fonseca Roberti , Carvalho Fredson Márcio Acris de , Marques André Augusto Franco
Published: April 2022

Abstract The purpose of this in vitro study was to evaluate the shaping ability of reciprocating and continuous rotary systems after root canal retreatment. After preparation and root canal filling, mesial canals of 54 mandibular molars were distributed into 3 groups (n=18), according to the filling material removal and re-instrumentation protocols: WOG group - WaveOne Gold system; PTN group - ProTaper Next system; and PTU group - ProTaper Universal system. Cone-beam computed tomographic (CBCT) images acquisition of the mesial root canals was performed at different moments: (1) before instrumentation (unprepared root canals), (2) after preparation and filling, (3) after filling material removal and (4) re-instrumentation. The apical transportation (AT), centering ability (CA) and change in root canal diameter were assessed by CBCT analysis. The remaining filling material quantification was performed by radiographic examination. The statistical analyses were performed using the 3-way ANOVA, Tukey-Kramer, Kruskal-Wallis and Dunn multiple Comparison tests (p<0.05). The tested instruments did not show full CA (=1.0). PTN group had greater AT at the 5th mm in comparison with the WOG group (p<0.05). After re-instrumentation, WOG group had greater root canal diameter change at the 1st and 5th mm than PTN and PTU groups (p<0.05). There was no significant difference among groups when comparing the amount of remaining filling material after re-instrumentation (p>0.05). The tested systems provided minimal alteration in root canal morphology at the apical portion after root canal retreatment. However, WOG promoted greater change in root canal diameter.
Ehlers-Danlos syndrome in a crossbreed cat
Authors: Caramalac Silvana Marques , Caramalac Simone Marques , Barbosa Carolynne Ferreira , Ferreira Julia Franco , Oliveira-Filho Jose Paes de , Borges Alexandre Secorun , Palumbo Mariana Isa Poci
Published: April 2022

ABSTRACT: The Ehlers-Danlos syndrome (EDS) consists of a group of diseases characterized by defective collagen production or failure in its organization, resulting in changes in the strength and extensibility of connective tissue. This report describes the dermatological and histological findings observed in a 3-month-old crossbreed cat with rupture and detachment of skin in the thoracic limb and rupture of the skin in the cervical region. Upon dermatological examination, the cat presented fragile and hyperextensible skin in the cervical region and a skin extensibility index of 21%. Histopathological evaluation of the skin specimens revealed evident disorganization of collagen bundles in dermis and in the Masson’s trichrome staining, follicular dysplasia was found. The presumptive diagnosis of EDS was made based on the clinical and histopathological findings. Sanger sequencing did not detect any mutated alleles for the c.3420delG mutation in COL5A1 gene, which was an autosomal dominant mutation previously been associated with Ehlers-Danlos syndrome in cats. The absence of this mutation in the reported cat suggests that other mutation may also be responsible for the development of cutaneous asthenia in this or maybe other genes related to collagen metabolism.
Impacto do tamanho das instituições de longa permanência na adesão às orientações de prevenção de infecções por COVID-19
Authors: Wachholz Patrick Alexander , Melo Ruth Caldeira de , Jacinto Alessandro Ferrari , Villas Boas Paulo José Fortes
Published: April 2022

Resumo Objetivo: avaliar a adesão das instituições de longa permanência brasileiras às orientações de Prevenção e Controle de Infecções da Organização Mundial da Saúde, observando a associação entre seu porte e a adesão a essas recomendações. Método: estudo transversal realizado com gestores de estabelecimentos. Os autores desenvolveram um questionário de 20 itens, com base nessas orientações, e um escore global de adesão, com base na adesão a essas recomendações. A adesão foi classificada como (1) excelente para aquelas que atenderam ≥14 de 20 recomendações, (2) bom para 10 a 13 itens e (3) baixo para aquelas com menos de dez itens. O tamanho das instalações foi classificado como pequeno, médio e grande, de acordo com uma análise de cluster em duas etapas. Estatística descritiva e teste de qui-quadrado foram utilizados com nível de significância de 5%. Resultados: das 362 instituições incluídas, 308 (85,1%) aderiram a 14 ou mais recomendações. Em relação ao seu tamanho, a adesão à triagem de sintomas de COVID-19 dos visitantes (p=0,037) e ao isolamento de pacientes até que tenham dois exames laboratoriais negativos (p=0,032) foi menor em estabelecimentos maiores, em comparação com estabelecimentos de médio e pequeno porte. Conclusão: a adesão às medidas de mitigação da COVID-19 nas unidades brasileiras foi considerada excelente para a maioria das recomendações, independentemente do porte das unidades.
Intersections between rural women’s resilience and quality of life: a mixed-methods study
Authors: Silva Bruno Neves da , Santos José Luís Guedes dos , Riquinho Deise Lisboa , Miranda Francisco Arnoldo Nunes de , Souza Nilba Lima de , Pinto Erika Simone Galvão
Published: April 2022

Abstract Objective: to analyze the intersections between rural women’s quality of life and resilience. Method: convergent mixed methods design in which a cross-sectional quantitative study is triangulated with a qualitative study guided by Oral History. Data were collected concomitantly, using a socio-demographic form, Resilience Scale, Medical Outcomes Study 36-Item Short-Form Health Survey, and open-ended interviews. The analysis was based on descriptive and inferential statistics and inductive thematic analysis, which was integrated later. Results: an association was found between the social aspects domain of quality of life and a moderate level of resilience related to the characteristics of life in rural areas. The integration of results enabled verifying that these two constructs (which mutually influence each other) are mediated by protective factors, resilience developed by the rural women, such as spirituality and the formation of social support, enchantment, and a feeling of belonging to their context. Conclusion: by developing protective factors, rural women develop a resilient behavior that favors their quality of life. Identifying these factors enables the development of psychosocial interventions to promote rural women’s health.
Isolation and molecular characterization of Cordyceps sp. from Bemisia tabaci (Hemiptera: Aleyrodidae) and pathogenic to Glycaspis brimblecombei (Hemiptera: Aphalaridae)
Authors: Domingues M. M. , Santos P. L. , Gêa B. C. C. , Carvalho V. R. , Oliveira F. N. , Soliman E. P. , Silva W. M. , Zanuncio J. C. , Santos Junior V. C. , Wilcken C. F.
Published: April 2022

Abstract The Brazilian forestry sector stands out for its technology, forestry management practices, social and environmental responsibility and, mainly, for its high productivity and exotic pests can reduce it. The red gum lerp psyllid Glycaspis brimblecombei (Moore, 1964) (Hemiptera: Aphalaridae) is an important pest in Eucalyptus plantations. The parasitoid Psyllaephagus bliteus (Riek, 1962) (Hymenoptera: Encyrtidae), predatory bugs and entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae are the natural enemies and used in the biological control of the red gum lerp psyllid. The use of entomopathogenic fungi against exotic pests is increasing in the forestry sector and the prospecting and identification of fungus isolates is important for integrated pest management. The objective of this work was the isolation and molecular identification of Cordyceps spp. And to evaluate the pathogenicity of isolates, obtained from Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) adults, against to the red gum lerp psyllid G. brimblecombei. The fungi were isolated from B. tabaci adults found in soybean and tomato crops and molecularly identified. The conidia obtained were suspended in solution with Tween 80 (0.1%) at a concentration of 1.0 × 108 conidia/mL and sprayed on ten G. brimblecombei nymphs per Eucalyptus leaf cut and placed on a hydroretentive gel inside per Petri dishes as a replication. The number of dead insects was quantified, daily, for seven days, and transferred to humid chambers. Cordyceps javanica (LCBPF 11) and C. fumosorosea (LCBPF 12 and LCBPF 63) were identified with a molecular analysis and all isolates were pathogenic to the insects and indicates that they could be used to manage G. brimblecombei and adds to reports that, normally, fungi cause greater mortality on insects of the same order as that from which they were isolated.
Molecular and morphological diversity, qualitative chemical profile and antioxidant activity of filamentous fungi of the digestive tract of Phylloicus sp. (Trichoptera: Calamoceratidae)
Authors: Romão T. C. , Menezes-Filho A. C. P. , Harakava R. , Castro C. F. S. , Morais P. B.
Published: April 2022

Abstract This study aimed to identify by molecular analysis, morphology, chemistry and antioxidant extracts of filamentous fungi isolated from the digestive tract of Phylloicus sp, an aquatic insect that lives on leaf packages in tropical streams and participates together with fungi of the decomposition of plant substrates in aquatic habitats. Insect larvae of Phylloicus sp. were collected in streams in the state of Tocantins, Brazil. Fungi were isolated from the digestive tract of larvae after disinfection and dissection, then described and purified for identification purposes and testing for antioxidant activity. Molecular identity was performed of ITS1 and ITS4, TUB e TEF sequencing. Fungal extracts were produced in 70% ethanol solution and later lyophilized. For analysis of chemical groups of extracts, thin layer chromatography (TLC) was performed in two mobile phases and different developers. Morphology was performed by optical microscopy stained with Toluidine Blue and measurement performed using the ImageJ program. Antioxidant activity performed in TLC and by quantitative method for DPPH and hydrogen peroxide (H2O2) radicals. Four fungi were identified: Endomelanconiopsis endophytica, Myxospora musae, Neopestalotiopsis cubana and Fusarium pseudocircinatum. The TLC showed several spots with acetone/chloroform mobile phase and UV 254 nm developers and I2 vapor. Fungal extracts demonstrate antioxidant action to reduce the DPPH free radical and especially for H2O2 above 50%, E. endophytica 91.6%, M. musae 87.8%, N. cubana 89.5% and 92.3% for F. pseudocircinatum. This study demonstrated that the molecular technique by PCR was satisfactory for identifying fungi, and extracts with numerous chemical groups and potent reducing agents. Thus future work, should be carried out evaluating these four species for industrial use.
Applicability of an immersive virtual reality system for assessing route learning in older adults
Authors: Santos Michelle Didone dos , Silva Juliana Magalhães da , Costa Raquel Quimas Molina da , Viveiro Larissa Alamino Pereira de , Moretto Emerson Galves , Lopes Roseli de Deus , Brucki Sonia Maria Dozzi , Pompeu José Eduardo
Published: April 2022

ABSTRACT. Spatial orientation is defined as the ability to find one’s way around an environment, follow familiar routes, recognize places, and learn new routes. Spatial disorientation is one of the early symptoms of Alzheimer’s disease (AD), and traditional cognitive evaluation lacks ecological validity. Therefore, new assessment methods are needed for the early identification of this cognitive impairment. Objective: This study aimed to compare the applicability and stability of an immersive virtual reality (VR) system developed to assess route learning between older adults with and without mild cognitive impairment (MCI). Methods: The study sample included 43 older adults: 22 without MCI and 23 with MCI. Applicability was assessed based on the recording of adverse events and the sense of presence reported through questionnaires. The Mann–Whitney U test was applied to compare the applicability of the Spatial Orientation in Immersive Virtual Environment Test (SOIVET)-Route task between older adults with and without MCI. Both short- and long-term stabilities of the task were evaluated using the intraclass correlation coefficient (ICC). Results: The mean age of participants was 71.4 years (SD=5.5). A minimum number of adverse events (mean=1.46; SD=2.11) and high levels of presence (mean=138.04; SD=14.80) were reported, and there was no difference between groups with and without MCI. A good to excellent correlation was found for short-term stability (CCI 0.78) and a reasonable correlation was found for long-term stability (CCI 0.58). Conclusions: The VR system was applicable for older adults and showed a good to excellent correlation for short-term stability.
Riegl faz história: o Denkmalkultus visto à luz da historiografia de Alois Riegl
Authors: PAZ DANIEL JURACY MELLADO
Published: April 2022

RESUMO O livro Der moderne Denkmalkultus, sein Wesen und seine Entsehung (O culto moderno dos monumentos: sua essência e sua gênese), do historiador da arte Alois Riegl (1858-1905), é hoje considerado uma das obras capitais do diálogo que chamamos de Teorias da Conservação e Restauro, e no Brasil estudado cada vez mais, com concepções inéditas e mesmo pioneiras em 1903, quando foi publicado. A leitura mais atenta do conjunto da obra de Riegl revela uma construção muito mais sofisticada do que aparece à leitura isolada do Denkmalkultus, como usual na área do Patrimônio. Em seus livros, Riegl explora e estabelece uma concepção particular de história e do ofício do historiador. Nela, diacronicamente, estão presentes a ênfase crescente da História como um devir contínuo, onde eram reconhecíveis linhas de sentido de longo prazo - como os vetores progressivos e reativos coexistentes em cada obra de arte -, e a possibilidade de épocas diferentes se tangenciarem, a partir do olhar para o passado, inclusive do historiador contemporâneo. Também se encontram, sincronicamente, as contribuições da História Cultural e o estudo das cosmovisões. Revela-se assim que o Denkmalkultus, como outros textos de Riegl, não era apenas uma descrição dos processos globais que acreditava existir desde o Paleolítico até a Era Moderna, como um exemplo concreto da progressão teleológica, essencial para interpretar sua obra.
A relação das ações preservacionistas na conduta do Ministério Público e da Municipalidade: a gestão do carnaval de Ouro Preto
Authors: SAYEGH LILIANE MÁRCIA LUCAS
Published: April 2022

RESUMO Este estudo busca analisar a relação entre a conduta do Ministério Público e da administração municipal no que diz respeito às ações de preservação do patrimônio cultural urbano e edificado frente aos desafios impostos pela realização anual do carnaval na cidade de Ouro Preto, MG. Para tanto, utiliza como pressupostos teóricos o breve estudo das competências de preservação cultural que cabem aos municípios e ao Ministério Público, além de discutir as escassas diretrizes voltadas à realização de eventos em núcleos tombados, e analisa as ações praticadas até o momento por essas instituições na cidade, no que tange à proteção do patrimônio em relação à realização do carnaval. Tais ações estão voltadas singularmente à execução de Termos de Ajustamento de Conduta entre as instituições citadas, bem como à regulamentação municipal e ao contexto de tais práticas, que revelam o papel que cada instituição tem desempenhado na gestão dos impactos relacionados ao evento na cidade. Além disso, este estudo ressalta como o caso avaliado tornou-se paradigmático para que se criassem diretrizes protecionistas para a realização do evento em outras cidades brasileiras, além de refletir sobre as possibilidades, limites e interações interinstitucionais do ponto de vista preservacionista, com o intuito de colaborar para estimular a discussão sobre a realização de eventos em espaços urbanos tombados no debate patrimonial.
Chemical composition and fermentation characteristics of maize silage with citrus pulp
Authors: SOUZA Amelina de Oliveira , TAVEIRA José Henrique da Silva , SANTOS Alessandro José Marques , FERNANDES Patrick Bezerra , COSTA Kátia Aparecida de Pinho , COSTA Carolina Marques , GURGEL Antonio Leandro Chaves , SILVA Ana Carolina Gomes , COSTA João Victor Campos Pinho
Published: April 2022

ABSTRACT The objective of this study was to evaluate the effects of including citrus pulp in maize silage on chemical composition and fermentation parameters. The experimental design was fully randomized with four replicates. Maize silage consisting of 0–50% citrus pulp was produced and analyzed. The pH linearly decreased with the increased inclusion of citrus pulp (3.49 at 50% citrus pulp), which is far below what is considered adequate (3.8–4.2). However, not all fermentation parameters were compromised. At 33% citrus pulp, we estimated 30.82 g/kg total nitrogen. At 49% citrus pulp, we estimated 95.16 g/kg of crude protein. At 21% and 22% citrus pulp, we estimated 549.89 g/kg of neutral detergent fiber and 678.11 g/kg of total digestible nutrients, respectively. Therefore, we conclude that the inclusion of up to 30% citrus pulp improves the complete chemical composition of maize silage owing to the reduction in structural carbohydrate values and increases in total digestible nutrient and protein content.
Trypsin inhibitor in Enterolobium contortisiliquum calli grown in the presence of plant growth regulators
Authors: Rezende Renata Alves Lara Silva , Rodrigues Filipe Almendagna , Ramos Vinicius de Oliveira , Martins Adalvan Daniel , Pasqual Moacir , Braga Júnior Roberto Alves , Luz José Magno Queiroz , Dória Joyce
Published: April 2022

Abstract The objective of this work was to investigate the effect of plant growth regulators (PGR) on the induction of calli from cotyledons of Enterolobium contortisiliquum grown in vitro, as well as to evaluate the inhibitory activity of trypsin in these calli. Cotyledons of E. contortisiliquum were cultivated in culture medium supplemented with dichlorophenoxyacetic acid (2,4-D), picloram (PIC), kinetin (KIN), and 6-benzylaminopurine (BAP). Callus mass, inhibitory activity of trypsin, morphogenetic and cytochemical observations, and biological activity were measured after 60 days. The Pareto diagram showed that only 0.5 mg L-1 PIC influenced the increase of fresh mass, whereas 0.5 mg L-1 BAP influenced dry mass. The principal component analysis plot showed the formation of two clear clusters. The inhibitory activity of trypsin is achieved at a higher intensity in the treatment with 0.5 mg L-1 2,4-D + 2.0 mg L-1 PIC + 0.5 mg L-1 KIN + 2.0 mg L-1 BAP. The combination of PGR at different rates causes an intense callus formation. Low concentrations of PGR result in a higher fresh mass, dry mass, and biological activity. The use of PGR is efficient in obtaining E. contortisiliquum calli with a satisfactory inhibitory activity of trypsin.
Structural analysis of soybean pods and seeds subjected to weathering deterioration in pre-harvest
Authors: Brzezinski Cristian Rafael , Abati Julia , Zucareli Claudemir , Medri Cristiano , Mertz-Henning Liliane Marcia , Krzyzanowski Francisco Carlos , França Neto José de Barros , Henning Fernando Augusto
Published: April 2022

Abstract The objective of this work was to analyze structurally the pods and seed coats of soybean (Glycine max) cultivars, as well as to determine the quality of seeds when subjected to deterioration by weathering in pre-harvest. A 7×3 factorial arrangement was used – seven cultivars and three volumes of simulated rainfall (0, 54, and 162 mm). Exposure to rainfall was simulated in the R8 phenological stage. Then, the plants were taken to a greenhouse, where they were kept until the time of collection of pods and seeds. After collection, the following evaluations were carried out: tetrazolium test, lignin content in pods and seed coats, and structural analysis of pods (exocarp, mesocarp, and endocarp thickness) and seed coats (epidermis, hypodermis, and parenchymal-cell thickness). Pre-harvest rainfall of 54 and 162 mm reduces the quality of soybean seeds; however, the response to deterioration by weathering differs according to the cultivar. The tolerance to all simulated rainfall was greater for BRSMT Pintado, BRS Jiripoca, and M8210IPRO and lower for BRS 1010IPRO. Pods with a greater thickness of the exocarp, mesocarp, and endocarp and a high lignin content show greater resistance to weathering deterioration and seeds with greater vigor and viability. The thickness of the hypodermis of the testa is related to resistance to weathering deterioration and to the obtainment of high-quality seeds.
O Conhecimento e a Prescrição da Profilaxia Pré-Exposição ao HIV por Médicos em Goiás
Authors: Bernardes Cristiane Teixeira Vilhena , Port Maria Eduarda , Freire Manuelle Quixabeira , Souza Izabella Gomes de , Rocha Julia Sousa , Borges Naiza Murielly Pereira , Freitas Yago José Fagundes de
Published: April 2022

A Síndrome da Imunodeficiência Adquirida é uma infecção que acomete o sistema imunológico e, para redução da propagação dessa patologia, desenvolveu-se os antirretrovirais. Evidências demonstram a eficácia da Profilaxia Pré-Exposição (PrEP), mas ainda com obstáculos, como a falta de conhecimento, técnica e treinamento dos profissionais de saúde para a sua orientação e prescrição. O objetivo dessa pesquisa é investigar o conhecimento subjetivo, objetivo e prescrição da PrEP por médicos do Estado de Goiás. Para a coleta de dados foi aplicado um questionário à 152 médicos com inscrição ativa no Conselho Regional de Medicina do Estado de Goiás. Como resultado, 132 médicos (86,8%) afirmaram possuir conhecimento prévio sobre a PrEP, em média 44,2% informaram ter conhecimento para prescrição, 129 (84,8%) nunca prescreveram PrEP e, quando avaliado objetivamente, em média 83,8% possuem conhecimento sobre a categorização de risco para o uso do medicamento. Os resultados dessa pesquisa não estão em consonância com a hipótese de que apenas 20% dos médicos teriam conhecimento suficiente, de acordo com a literatura, mas é evidente a necessidade de capacitação médica visando otimizar o impacto clínico na saúde pública desta importante estratégia de prevenção do HIV.
Transient creep strain of fly ash concrete at elevated temperatures
Authors: Fan Kunjie , Li Jiabin , He Zhihai , Liu Qingfeng , Yao Yao
Published: April 2022

Accurate modelling of transient creep strain (TRC) is crucial for reliable fire performance assessments of concrete structures, since it is the largest strain component for structural concrete subjected to thermal exposure. Nonetheless, the mechanism underlying TRC is still not fully understood and most of the available models were established based on ordinary Portland cement concrete. As the most widely used supplemental cementitious material, the effect of fly ash on the development of TRC still requires further study. For this paper, steady state tests and transient state tests were carried out on ordinary Portland cement concrete (CEM I 52.5 as per BS EN 197-1, water/cement = 0.5) and fly ash concrete (class F fly ash as per BS EN 450-1, 25% replacement percentage), respectively. It was found that replacing 25% ordinary Portland cement with class F fly ash in concrete can mitigate the development of TRC above 400°C. Based on the experimental results, one assumption of the influence mechanism of class F fly ash on TRC is made and a TRC model for fly ash concrete (class F, 25% replacement percentage) at elevated temperatures is proposed.
Carbon nitride prepared through a combined strategy of electrophoresis and vapor deposition
Authors: Xie Zhiqiang , Wang Yuqing , Peng Wei , Yi Zhehan , Zhang Tao , Si Wenping , Hou Feng
Published: April 2022

Photoelectrochemical water splitting converts solar energy into clean hydrogen fuel. In this system, the preparation of high-quality photoanodes is essential for achieving efficient solar hydrolysis. Polymeric carbon nitride has emerged as a promising photocatalyst for solar water splitting due to its visible light absorption, suitable band edge positions and good chemical stability. However, considerable difficulties have been encountered in the preparation of robust and well-bonded carbon nitride films on conductive substrates. Herein, a combined strategy of electrophoresis and vapor deposition results in robust and high-performing carbon nitride film photoelectrodes with good bonding on substrates, suitable thickness for light absorption and enhanced charge separation and transfer abilities. The unique structure of the photoanode thus facilitates the oxidation of water and leads to an optimal photocurrent density of approximately 120 μA/cm2 at 1.23 VRHE. This work provides new insights for the preparation of polymeric carbon nitride film photoelectrodes, promoting the developments of photoelectric and photoelectrochemical applications based on carbon nitride.
Interleukin-33 Exacerbates IgA Glomerulonephritis in Transgenic Mice Overexpressing B Cell Activating Factor
Authors: Wang Yuan Min , Shaw Karli , Zhang Geoff Yu , Chung Edmund Y.M. , Hu Min , Cao Qi , Wang Yiping , Zheng Guoping , Wu Huiling , Chadban Steven J. , McCarthy Hugh J. , Harris David C.H. , Mackay Fabienne , Grey Shane T. , Alexander Stephen I.
Published: April 2022

Significance Statement

IL-33 is an activator of innate lymphoid cells 2 (ILC2s) in innate immunity and allergic inflammation and drives ILC2 expansion. High levels of B cell activating factor (BAFF), which plays a central role in B cell proliferation and differentiation, cause excess antibody production, including IgA. Using an animal model of IgA glomerulonephritis, a transgenic mouse that expresses excessive BAFF, the authors found that IL-33 exacerbates IgA glomerulonephritis, appearing to do so through expansion of ILC2 cells that drive an increase in IgA production and the development of kidney disease. They confirmed the role of ILC2s in exacerbating disease in a mouse model of IgA glomerulonephritis in transfer and depletion experiments. Their findings suggest that evaluation of IL-33 and ILC2s as potential mediators of IgA nephropathy in humans is warranted.

Background

The cytokine IL-33 is an activator of innate lymphoid cells 2 (ILC2s) in innate immunity and allergic inflammation. B cell activating factor (BAFF) plays a central role in B cell proliferation and differentiation, and high levels of this protein cause excess antibody production, including IgA. BAFF-transgenic mice overexpress BAFF and spontaneously develop glomerulonephritis that resembles human IgA nephropathy.

Methods

We administered IL-33 or PBS to wild-type and BAFF-transgenic mice. After treating Rag1-deficient mice with IL-33, with or without anti-CD90.2 to preferentially deplete ILC2s, we isolated splenocytes, which were adoptively transferred into BAFF-transgenic mice.

Results

BAFF-transgenic mice treated with IL-33 developed more severe kidney dysfunction and proteinuria, glomerular sclerosis, tubulointerstitial damage, and glomerular deposition of IgA and C3. Compared with wild-type mice, BAFF-transgenic mice exhibited increases of CD19+ B cells in spleen and kidney and ILC2s in kidney and intestine, which were further increased by administration of IL-33. Administering IL-33 to wild-type mice had no effect on kidney function or histology, nor did it alter the number of ILC2s in spleen, kidney, or intestine. To understand the role of ILC2s, splenocytes were transferred from IL-33–treated Rag1-deficient mice into BAFF-transgenic mice. Glomerulonephritis and IgA deposition were exacerbated by transfer of IL-33–stimulated Rag1-deficient splenocytes, but not by ILC2 (anti-CD90.2)–depleted splenocytes. Wild-type mice infused with IL-33–treated Rag1-deficient splenocytes showed no change in kidney function or ILC2 numbers or distribution.

Conclusions

IL-33–expanded ILC2s exacerbated IgA glomerulonephritis in a mouse model. These findings indicate that IL-33 and ILC2s warrant evaluation as possible mediators of human IgA nephropathy.
Gesundheitliche Risiken durch Schichtarbeit – immer noch ein Thema für die Prävention? (Interview)
Authors:   Zentralinstitut für Arbeitsmedizin und Maritime Medizin (ZfAM), Universitätsklinikum Hamburg- Eppendorf (UKE), Harth Volker
Published: April 2022

Univ.-Prof. Dr. Volker Harth, MPH, ist seit 2013 Direktor des Zentralinstituts für Arbeitsmedizin und Maritime Medizin (ZfAM) in Hamburg und hat die Universitätsprofessur für Arbeitsmedizin und Maritime Medizin am Universitätsklinikum Hamburg-Eppendorf (UKE) inne. Ein Forschungsschwerpunkt seiner wissenschaftlichen Arbeiten sind die gesundheitlichen Auswirkungen von Nacht- und Wechselschichtarbeit. Im Dezember 2020 wurde die S2K-Leitlinie „Gesundheitliche Aspekte und Gestaltung von Nacht- und Schichtarbeit“, in der er als Leitlinienkoordinator fungierte, durch die Arbeitsgemeinschaft der Wissenschaftlichen Fachgesellschaften (AWMF) veröffentlicht. ASU sprach mit ihm über die Bedeutung der Nacht- und Wechselschichtarbeit für die Gesundheit der Beschäftigten.
Mobile Arbeit und Homeoffice
Authors:   Bundesvereinigung der Deutschen Arbeitgeberverbände (BDA), Berlin, Clauß Elisa
Published: April 2022

Die Coronazeit hat mobile Arbeit und ihre spezielle Ausprägung, das Homeoffice, in den Vordergrund gebracht. Wie aber wird das Homeoffice der Zukunft aussehen, wenn es ein freiwilliges Angebot der Unternehmen an ihre Beschäftigten ist und keine Maßnahme des Infektionsschutzes? Der folgende Betrag liefert einen Einblick in die Chancen und Lösungsideen, greift aber auch Fragen und Herausforderungen auf, denen sich Unternehmen hinsichtlich dieser Thematik stellen müssen.
Research As Development: Book Forum
Authors: Widger Tom , Tousignant Noémi , Eddleston Michael , Dawson Andrew , Senanayake Nari , Sariola Salla , Simpson Bob
Published: April 2022

Book forum on 'Research as Development'
Crowds and COVID-19: An Introduction
Authors: Saria Vaibhav , Satyogi Pooja
Published: April 2022

Introduction to the Special Section 'Crowds and COVID-19', guest edited by Vaibhav Saria and Pooja Satyogi.
Boş Zaman Becerikliliği Ölçeğinin Türkçeye Uyarlanması: Geçerlik ve Güvenirlik Çalışması
Authors: SÜRÜCÜ Lütfi , ERTAN Şenay Sahil
Published: April 2022

Boş zaman becerikliliğine yönelik literatürde birçok çalışma olmakla birlikte bu araştırmaların çoğu nitel çalışmalardan oluşmaktadır. Petrou ve Bakker (2016) tarafından geliştirilen ölçekle beraber boş zaman becerikliliği nicel olarak da ölçmek mümkün olmuştur. Petrou ve Bakker (2016) tarafından geliştirilen ölçek daha öncesinde Türkçeye uyarlanmamıştır. Bu doğrultuda, çalışmanın amacı ise Petrou ve Bakker (2016) tarafından geliştirilen boş zaman becerikliliği ölçeğini Türkçeye uyarlamak ve çevirmektir. Bu maksatla İstanbul’da özel üniversitelerde okuyan 391 öğrenciye anket uygulanmıştır. Ölçeğin geçerliliği ve güvenilirliği elde edilen verilere göre test edilmiştir. Bulgular, boş zaman becerikliliği ölçeğinin güvenilir ve geçerli bir ölçüm aracı olduğunu ve Türkiye'deki örnek araştırma gruplarında kullanılabileceğini göstermiştir.
Patch-Seq Internal Solution with Biocytin v2
Authors: Institute for Brain Science Allen
Published: April 2022

This SOP provides instruction to prepare Internal Solution which is used for patch clamp electrophysiology, modified for cleanliness and mRNA capture and sustainability. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
scRNA-seq Data Processing (Cell Ranger, v6.0.0) v1
Authors: not provided molmer , Lotz Martin , Natarajan Padmaja , Head Steven
Published: April 2022

scRNA-seq data are analyzed using Cell Ranger from 10X Genomics.
[RETRACTED] Sports Illustrated Intensi-T Test Booster Reviews - Is This a Scam or Legit Product [2022 Update] v1
Authors: not provided dixiespaul
Published: April 2022

[RETRACTED]

Sports Illustrated Intensi-T Test Booster
Optimizing the electroporation parameters forHeterometopus palaeformis (strain RAJCA) v1
Authors: Gomaa Fatma , Rotterova Johana , Beinart Roxanne , Pachiadaki Maria , Edgcomb Virginia
Published: April 2022

Cell viability after incubation with different electroporation buffers, Phosphate buffered saline (PBS) is used as an electroporation buffer for many eukaryotic cells. However, we observed that the PBS has a detrimental effect on the cell, causing disruption in H. palaeformis cell membrane. We then tested the tolerance of H. palaeformis to three different electroporation buffers: A-Gene Pulser Electroporation Buffer(BIO-RAD, USA) B-Iso-osmolar Buffer (Eppendorf, USA) C-Hypo-osmolar Buffer (Eppendorf, USA) Cells were incubated in each buffer independently for 10 minutes and observed for their viability under inverted microscopy. For all the three tested buffers, we noticed that once buffer was added, the cells showed instant osmic shock, include loss of motility and relative shrinking in the cell membrane (Fig. 1). After 10 minutes incubation period in each buffer, cells were transferred to their Cerophyl culture medium to recover. We observed progressive recovery of the cells. Table 1 shows the time that took the cells to completely recovered and the percentages of the recovered cells after incubation at the three different electroporation buffers. Table 1 Electroporation buffer Duration of recovery Percentage of the recovered cells Gene Pulser 10 minutes >90% Iso-osmolar 10 minutes 70% Hypo-osmolar 2-5 minutes 70% Fig.1. Light microscopy images showing the effect of the different electroporation buffer on the H. palaeformis cell membrane; PBS has detrimental effect on the cell causing disruption of cell membrane.
Clustering of Fifth Generation Mutants of Genetically - Improved Kipas Putih Soybean with High Protein Content
Authors: Zuyasna Zuyasna , Andari Risliawati Andari Risliawati , Gunawan Gunawan
Published: April 2022

Kipas Putih is one of the local varieties of soybean from Aceh, Indonesia. To improve the productivity of this variety, the irradiation mutation technique was applied. This study aimed to examine the level of genetic diversity in the M5 generation of Kipas Putih soybean using SSR markers and to identify whether any used markers were associated with the loci that control the protein content in soybean. A total of 18 SSR markers were used to amplify the DNA of 11 mutant lines of Kipas Putih. The total alleles detected were 55 alleles which range from 1 to 7 alleles per locus. The average of major allele’s frequency was 0.71 with the heterogeneity of 0.17. The PIC value ranged from 0.00 to 0.73 with an average of 0.35. Satt308 was identified as the most polymorphic marker among others since it had a PIC value greater than 0.70. The genetic distance analysis showed that the mutant lines B4, B10 and B13 had the furthest genetic distance (0.45) with the Kipas Putih (B0) soybean variety. The phylogenetic analysis showed Kipas Putih soybean and ten mutants of M5 generation grouped into four main clusters at a similarity coefficient of 0.20. Furthermore, association analysis showed that only two SSR markers, Satt577 and Satt431 were significantly associated with protein content with an R2 value of 0.7855 to 0.9643.
Effect of Protein A-Immunoadsorption Therapy on Serum Cytokines in AAV Patients
Authors: Li Xiejia , Li Zheng , Deng Hongmei , Sun Cuifang , Liu Hong , Yuan Fang
Published: April 2022

Protein A-immunoadsorption (IA) is an extracorporeal apheresis technique used in patients with autoimmune diseases which aims to remove pathogenic autoantibodies. Apart from the adsorption of immunoglobulins (IgG), IA may influence cellular and humoral immunity. The aim of this study was to observe the effect of protein A-IA on cytokine networks in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) patients. A total of 12 newly diagnosed AAV patients received IA therapy on days 1, 3, and 5. Serum levels of inflammatory cytokines were measured before and after treatment including members of the interleukin family, PDGF-BB, TNF-α and IFN-γ. IL-1β, IL-9, IL-17A, PDGF-BB, IFN-γ, and TNF-α were elevated in AAV patients compared to healthy individuals. There were no changes in the levels of any cytokines after the first IA session but after 3 sessions, IL-9, PDGF-BB, and TNF-α dramatically decreased. Moreover, reductions in IL-9 levels were positively correlated with the changes of myeloperoxidase-ANCA (MPO-ANCA). Our observations suggest that protein A-IA therapy does not directly adsorb cytokines, but removes autoantibodies (MPO-ANCA) which indirectly leads to changes in cytokine networks linked to cellular or humoral immunity.
The Occurred Error in Microdosimetry Calculations while using Water Instead of the Body Organs in Proton Therapy and a New Formula for an Estimate of the Statistical Uncertainty of Microdosimetric Quantities
Authors: Jahanfar Somayeh , Tavakoli-Anbaran Hossein
Published: April 2022

In many experimental and simulation researches, water phantom is used instead of most body organs. Therefore, in this study, we replaced the water phantom instead of some organs to calculate its effect on the proton stopping-power, and range and the consequence of deposited energy and microdosimetric spectra in small sites. Some organs such as the spleen, thyroid, pancreas, prostate, testis, and ovaries are considered. We calculated the proton stopping-power in these organs using the SRIM code. Then using these results, we wrote a program in the programming language of Fortran and computed the proton range and deposited energy in two sites of 1 and 100 micron. Also, using the Geant4-10-4 code, we simulated these sites and obtained microdosimetric spectra of protons at 1 and 5MeV energies. In order to compare different states, the frequency-mean lineal energy, dose-mean lineal energy, these statistical uncertainties and absorb dose in each case were calculated and reported. Also, we estimated the statistical uncertainty of quantities with a new formula. We observed that using water instead of the organs causes a significant error in the calculations of the range and the maximum relative difference percentage of 18% and 22% in deposited energy in 1 and 100 micron sites, respectively. These differences depend on the energy of the incident proton, organ, and size site. Also, this replacement changes microdosimetric spectra, the location, and intensity of the Bragg's peak. The percent difference of location and intensity of the Bragg's peak for water instead of the spleen is -8.66 and 13.42%, respectively. Therefore, using water instead of the body organs in microdosimetry calculations is not recommended.
No Time to Lose Sex-Appeal
Authors: Duque Sanchez Elena , López de Aguileta Garazi , Maria Canal Josep , Foraster Mar Joanpere
Published: April 2022

The James Bond films have had great impact in society from the first one to the most recent one (1962-2021). Much research has been conducted on them, particularly about masculinity and femininity representations. Bond habitually embodies a model of masculinity that disdains women in repetitive dynamics in each film disguised as a halo of seduction. It is identified that this masculinity model has changed in the last films towards a more sensitive and less sexist one. However, the audience’s perception of Bond’s attractiveness according to this change has not been analyzed in depth. This paper aims at studying people’s perceptions of Bond’s attractiveness as the character’s way of treating women changes, particularly in the last film No time to die. To that end, film reviews and user comments in digital platforms were revised. Then, 6 interviews have been conducted. Results show that the language of ethics is used mostly to refer to the correctness of Bond’s changes. The language of desire is mainly used to express a preference for the attractiveness of the traditional model of Bond’s masculinity. A discussion about the language of ethics and the language of desire related to the coercive discourse is made proposing further research.
A Systems Approach to Studying Online Communities
Authors: Foote Jeremy
Published: April 2022

Much early communication research was inspired by systems theory. This approach emphasizes that individuals and groups use communication to interact with and respond to their larger environment and attempts to outline the ways that different levels interact with each other (e.g., work groups within departments within firms). Many concepts from systems theory—such as emergence and feedback loops—have become integral parts of communication theories. However, until recently, quantitative researchers have struggled to apply a systems approach. Large-scale, multilevel trace data from online platforms combined with computational advances are enabling a turn back toward systems-inspired research. I outline four systems-based approaches that recent research uses to study online communities: community comparisons, individual trajectories, cross-level mechanisms, and simulating emergent behavior. I end with a discussion of the opportunities and challenges of systems-based research for quantitative communication scholars.
Identification and Classification of Toxic Statements by Machine Learning Methods
Authors: Platonov E.N. , Rudenko V.Y.
Published: April 2022

The number of comments left on social media platforms can reach several million per day, so their owners are interested in automatic content filtering. In this paper, the task of identifying offensive statements in texts is considered. When solving the problem, various methods of vector text conversion were considered: TF-IDF, Word2Vec, Glove, etc. The results of the application of classical text classification methods and neural network methods (LSTM, CNN) were also considered and presented.
Automatic Establishment of Generic Relationships Between Concepts
Authors: Makarova A.E. , Nikitin Y.V. , Khoroshilov A.A.
Published: April 2022

The article describes the results of research on the creation of methods for constructing a formalized semantic description of documents for solving problems of semantic search for documents. The methods used in the study are based on the use of machine grammar procedures and conceptual analysis of texts, which ensure the identification of its conceptual composition and the assignment of characteristics to the names of concepts that correspond to their semantic role and significance in the text. To perform this work, a set of software tools was created, which was tested on media documents.
The “Regulatory Guillotine” Mechanism in Education: Problems and Prospects of Practical Implementation in the Work of the Federal Service for Supervision in the Field of Education and Science
Authors: Kirillovykh A. A.
Published: April 2022

The paper analyzes the control-supervisory and administrative practices of the Federal Service for Supervision in the Field of Education and Science (Rosobrnadzor of Russia) in relation to the activities of educational institutions of higher education. In the context of the review of verification measures, the paper provides an assessment of the current level, as well as the prospects for the effectiveness of the implementation of the regulatory concept of deregulation and reducing the administrative burden on controlled entities within the framework of the conceptual (main) provisions of the administrative reform. It is concluded that control and supervision activities in the field of education should be consistent with the principle of encouraging conscientious compliance by educational organizations with mandatory requirements. When forming the practice of sustainable conscientious behavior by educational organizations within the framework of their statutory activities, Rosobrnadzor has the opportunity to use incentive measures for such entities aimed at solving the tasks of ensuring the socioeconomic development of individual territories without reducing the quality of educational services provided by them.