bluuebunny/crossref_metadata_2025 huggingface dataset:
Practicing Dentists’ Self-Efficacy and Associated Factors in Managing the Treatment of Adults with Mental and Physical Disabilities: An Exploratory Cross-Sectional Study in Riyadh, Saudi Arabia
Authors: AlQahtani Sakher , Murshid Ebtissam , Fadel Hani , Kassim Saba
Published: December 2017

Background: Provision of oral healthcare to adults with mental and physical disabilities (AMPD) remains a challenging area across various healthcare systems. The present study aimed to assess self-reported efficacy and investigate factors associated with self-efficacy in the management of AMPD among practicing dentists in Saudi Arabia. Methods: A pilot-tested, self-administered questionnaire was distributed to a convenience sample of 1000 dentists. Descriptive and inferential analyses were performed on the collected responses. Results: Among the respondents (54%), 43% were males. Only 14% described their self-efficacy in managing AMPD as “High”. Multivariable regression analyses revealed significant associations between “High” self-efficacy and male gender (Odd ratio (OR) = 2.39, 95% CI = 1.16–4.89), experience practicing dentistry for 11 years or more (OR = 2.19, 95% CI = 1.04–4.47), specialization in pediatric dentistry (OR = 3.98, 95% CI = 1.31–12.07), previous experience in managing AMPD (OR = 4.23, 95% CI = 1.59–11.22), and awareness of regulations for managing AMPD (OR = 2.62, 95% CI = 1.42–4.38). The interaction of gender x age was significantly associated (p = 0.028) with “High” self-efficacy. Gender-stratified analyses revealed a significant association between age and “High” self-efficacy among male dentists only. Conclusions: The findings of the present study highlight that a large percentage of dentists reported “Low/Moderate” self-efficacy in managing AMPD, particularly among female dentists. However, further studies are required to verify the factors associated with self-efficacy in the present study, and to identify other factors that may influence self-efficacy in managing AMPD.
Food (In)Security in Rapidly Urbanising, Low-Income Contexts
Authors: Tacoli Cecilia
Published: December 2017

Urbanisation in low and middle-income nations presents both opportunities and immense challenges. As urban centres grow rapidly, inadequate housing and the lack of basic infrastructure and services affect a large and growing proportion of their population. There is also a growing body of evidence on urban poverty and its links with environmental hazards. There is, however, limited knowledge of how these challenges affect the ways in which poor urban residents gain access to food and secure healthy and nutritious diets. With some important exceptions, current discussions on food security continue to focus on production, with limited attention to consumption. Moreover, urban consumers are typically treated as a homogenous group and access to food markets is assumed to be sufficient. This paper describes how, for the urban poor in low and middle-income countries, food affordability and utilisation are shaped by the income and non-income dimensions of poverty that include the urban space.
Analgesic-Like Activity of Essential Oil Constituents: An Update
Authors: De Cássia da Silveira e Sá Rita , Lima Tamires , Da Nóbrega Flávio , De Brito Anna , De Sousa Damião
Published: December 2017

The constituents of essential oils are widely found in foods and aromatic plants giving characteristic odor and flavor. However, pharmacological studies evidence its therapeutic potential for the treatment of several diseases and promising use as compounds with analgesic-like action. Considering that pain affects a significant part of the world population and the need for the development of new analgesics, this review reports on the current studies of essential oils’ chemical constituents with analgesic-like activity, including a description of their mechanisms of action and chemical aspects.
Of Energy and Entropy: The Ineluctable Impact of Aging in Old Age Dementia
Authors: Boccardi Virginia , Comanducci Chiara , Baroni Marta , Mecocci Patrizia
Published: December 2017

Alzheimer’s disease (AD) represents the most common form of dementia among older age subjects, and despite decades of studies, the underlying mechanisms remain unresolved. The definition of AD has changed over the past 100 years, and while early-onset AD is commonly related to genetic mutations, late-onset AD is more likely due to a gradual accumulation of age-related modifications. “Normal brain aging” and AD may represent different pathways of successful or failed capability to adapt brain structures and cerebral functions. Cellular senescence and age-related changes (ARCs) affecting the brain may be considered as biologic manifestations of increasing entropy, a measure of disorder. Late-onset AD may be regarded as the final effect of a reduced energy production, due to exhausted mitochondria, and an increased entropy in the brain. This unique trajectory enables a bioenergetics-centered strategy targeting disease-stage specific profile of brain metabolism for disease prevention and treatment.
Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells—A Primary Culture Approach
Authors: Kranc Wiesława , Brązert Maciej , Ożegowska Katarzyna , Nawrocki Mariusz , Budna Joanna , Celichowski Piotr , Dyszkiewicz-Konwińska Marta , Jankowski Maurycy , Jeseta Michal , Pawelczyk Leszek , Bruska Małgorzata , Nowicki Michał , Zabel Maciej , Kempisty Bartosz
Published: December 2017

Because of the deep involvement of granulosa cells in the processes surrounding the cycles of menstruation and reproduction, there is a great need for a deeper understanding of the ways in which they function during the various stages of those cycles. One of the main ways in which the granulosa cells influence the numerous sex associated processes is hormonal interaction. Expression of steroid sex hormones influences a range of both primary and secondary sexual characteristics, as well as regulate the processes of oogenesis, folliculogenesis, ovulation, and pregnancy. Understanding of the exact molecular mechanisms underlying those processes could not only provide us with deep insight into the regulation of the reproductive cycle, but also create new clinical advantages in detection and treatment of various diseases associated with sex hormone abnormalities. We have used the microarray approach validated by RT-qPCR, to analyze the patterns of gene expression in primary cultures of human granulosa cells at days 1, 7, 15, and 30 of said cultures. We have especially focused on genes belonging to ontology groups associated with steroid biosynthesis and metabolism, namely “Regulation of steroid biosynthesis process” and “Regulation of steroid metabolic process”. Eleven genes have been chosen, as they exhibited major change under a culture condition. Out of those, ten genes, namely STAR, SCAP, POR, SREBF1, GFI1, SEC14L2, STARD4, INSIG1, DHCR7, and IL1B, belong to both groups. Patterns of expression of those genes were analyzed, along with brief description of their functions. That analysis helped us achieve a better understanding of the exact molecular processes underlying steroid biosynthesis and metabolism in human granulosa cells.
Population-Specific Associations of Deleterious Rare Variants in Coding Region of P2RY1–P2RY12 Purinergic Receptor Genes in Large-Vessel Ischemic Stroke Patients
Authors: Janicki Piotr , Eyileten Ceren , Ruiz-Velasco Victor , Sedeek Khaled , Pordzik Justyna , Czlonkowska Anna , Kurkowska-Jastrzebska Iwona , Sugino Shigekazu , Imamura-Kawasawa Yuka , Mirowska-Guzel Dagmara , Postula Marek
Published: December 2017

The contribution of low-frequency and damaging genetic variants associated with platelet function to ischemic stroke (IS) susceptibility remains unknown. We employed a deep re-sequencing approach in Polish patients in order to investigate the contribution of rare variants (minor allele frequency, MAF < 1%) to the IS genetic susceptibility in this population. The genes selected for re-sequencing consisted of 26 genes coding for proteins associated with the surface membrane of platelets. Targeted pooled re-sequencing (Illumina HiSeq 2500) was performed on genomic DNA of 500 cases (patients with history of clinically proven diagnosis of large-vessel IS) and 500 controls. After quality control and prioritization based on allele frequency and damaging probability, follow-up individual genotyping of deleterious rare variants was performed in patients from the original cohort. Gene-based analyses identified an association between IS and 6 rare functional and damaging variants in the purinergic genes (P2RY1 and P2RY12 locus). The predicted properties of the most damaging rare variants in P2RY1 and P2RY12 were confirmed by using mouse fibroblast cell cultures transfected with plasmid constructs containing cDNA of mutated variants (FLIPR on FlexStation3). This study identified a putative role for rare variants in P2RY1 and P2RY12 genes involved in platelet reactivity on large-vessel IS susceptibility in a Polish population.
An Oldie but Goodie: Lithium in the Treatment of Bipolar Disorder through Neuroprotective and Neurotrophic Mechanisms
Authors: Won Eunsoo , Kim Yong-Ku
Published: December 2017

Lithium has been used for the treatment of bipolar disorder (BD) for the last sixty or more years, and recent studies with more reliable designs and updated guidelines have recommended lithium to be the treatment of choice for acute manic, mixed and depressive episodes of BD, along with long-term prophylaxis. Lithium’s specific mechanism of action in mood regulation is progressively being clarified, such as the direct inhibition on glycogen synthase kinase 3β, and its various effects on neurotrophic factors, neurotransmitters, oxidative metabolism, apoptosis, second messenger systems, and biological systems are also being revealed. Furthermore, lithium has been proposed to exert its treatment effects through mechanisms associated with neuronal plasticity. In this review, we have overviewed the clinical aspects of lithium use for BD, and have focused on the neuroprotective and neurotrophic effects of lithium.
Significant Down-Regulation of “Biological Adhesion” Genes in Porcine Oocytes after IVM
Authors: Budna Joanna , Celichowski Piotr , Bryja Artur , Dyszkiewicz-Konwińska Marta , Jeseta Michal , Bukowska Dorota , Antosik Paweł , Brüssow Klaus Peter , Bruska Małgorzata , Nowicki Michał , Zabel Maciej , Kempisty Bartosz
Published: December 2017

Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes’ maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB+ oocytes were directly exposed to microarray assays and RT-qPCR (“before IVM” group), or first in vitro matured and then if classified as BCB+ passed to molecular analyses (“after IVM” group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes’ successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte’s achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.
DocCreator: A New Software for Creating Synthetic Ground-Truthed Document Images
Authors: Journet Nicholas , Visani Muriel , Mansencal Boris , Van-Cuong Kieu , Billy Antoine
Published: December 2017

Most digital libraries that provide user-friendly interfaces, enabling quick and intuitive access to their resources, are based on Document Image Analysis and Recognition (DIAR) methods. Such DIAR methods need ground-truthed document images to be evaluated/compared and, in some cases, trained. Especially with the advent of deep learning-based approaches, the required size of annotated document datasets seems to be ever-growing. Manually annotating real documents has many drawbacks, which often leads to small reliably annotated datasets. In order to circumvent those drawbacks and enable the generation of massive ground-truthed data with high variability, we present DocCreator, a multi-platform and open-source software able to create many synthetic image documents with controlled ground truth. DocCreator has been used in various experiments, showing the interest of using such synthetic images to enrich the training stage of DIAR tools.
Accelerated Benders’ Decomposition for Integrated Forward/Reverse Logistics Network Design under Uncertainty
Authors: Vahdat Vahab , Vahdatzad Mohammad Ali
Published: December 2017

In this paper, a two-stage stochastic programming modelling is proposed, to design a multi-period, multistage, and single-commodity integrated forward/reverse logistics network design problem under uncertainty. The problem involved both strategic and tactical decision levels. The first stage dealt with strategic decisions, which are the number, capacity, and location of forward and reverse facilities. In the second stage, tactical decisions, such as base stock level as an inventory policy, were determined. The generic introduced model consisted of suppliers, manufactures, and distribution centers in forward logistic and collection centers, remanufactures, redistribution, and disposal centers in reverse logistic. The strength of the proposed model is its applicability to various industries. The problem was formulated as a mixed-integer linear programming model and was solved by using Benders’ Decomposition (BD) approach. In order to accelerate the Benders’ decomposition, a number of valid inequalities were added to the master problem. The proposed accelerated BD was evaluated through small-, medium-, and large-sized test problems. Numerical results confirmed that the proposed solution algorithm improved the convergence of BD lower bound and the upper bound, enabling to reach an acceptable optimality gap in a convenient time.
Formation of Surface and Quantum-Well States in Ultra Thin Pt Films on the Au(111) Surface
Authors: Silkin Igor , Koroteev Yury , Echenique Pedro , Chulkov Evgueni
Published: December 2017

The electronic structure of the Pt/Au(111) heterostructures with a number of Pt monolayers n ranging from one to three is studied in the density-functional-theory framework. The calculations demonstrate that the deposition of the Pt atomic thin films on gold substrate results in strong modifications of the electronic structure at the surface. In particular, the Au(111) s-p-type Shockley surface state becomes completely unoccupied at deposition of any number of Pt monolayers. The Pt adlayer generates numerous quantum-well states in various energy gaps of Au(111) with strong spatial confinement at the surface. As a result, strong enhancement in the local density of state at the surface Pt atomic layer in comparison with clean Pt surface is obtained. The excess in the density of states has maximal magnitude in the case of one monolayer Pt adlayer and gradually reduces with increasing number of Pt atomic layers. The spin–orbit coupling produces strong modification of the energy dispersion of the electronic states generated by the Pt adlayer and gives rise to certain quantum states with a characteristic Dirac-cone shape.
In Vitro Investigation of Wear of CAD/CAM Polymeric Materials Against Primary Teeth
Authors: Choi Jae-Won , Song Eun-Ju , Shin Jong-Hyun , Jeong Tae-Sung , Huh Jung-Bo
Published: December 2017

The aim of the study was to evaluate the effects of polymeric computer-aided design/computer-aided manufacturing CAD/CAM materials on antagonistic primary tooth wear. Five CAD/CAM polymeric materials were examined: Vipi Block Monocolor (VBM), Yamahachi polymethylmethacrylate (PMMA) (YAP), Mazic Duro (MZD), Vita Enamic (ENA), and Pekkton (PEK). All of the specimens were tested in a thermomechanical loading machine with the primary canine as the antagonist (50 N, 1.2 × 105 cycles, 1.7 Hz, 5/55 °C). The wear losses of the antagonist tooth and the restorative materials were calculated using reverse modelling software and an electronic scale. VBM and ENA showed significantly higher antagonist tooth wear than PEK (p < 0.05), but there was no significant difference observed among VBM, YAP, MZD, and ENA (p > 0.05). PEK showed the largest value in both material volumetric and weight losses. In terms of material volumetric losses, there was no significant difference between all of the groups (p > 0.05). In terms of material weight losses, PEK was significantly larger than ENA (p < 0.05), but there was no significant difference between VBM, YAP, MZD, and ENA (p > 0.05). Volumetric and weight losses of materials showed similar wear behaviour. However, the wear patterns of antagonists and materials were different, especially in PEK.
Alkaline Material Effects on Roots of Teeth
Authors: Shetty Sowmya , Kahler Sam , Kahler Bill
Published: December 2017

The aim of this review was to identify and analyse all studies related to the effects of alkaline materials used in dentistry on roots of teeth. The first part of the review focused on mechanical property alterations of root dentine due to sodium hypochlorite (SH) used as an irrigant solution based on MeSH (Medical Subject Heading) terms from a previous study by Pascon et al in 2009. The second part reviewed literature on calcium hydroxide (CH), mineral trioxide aggregate (MTA) and other alkaline materials used as root canal dressings or filling materials. Additional MeSH terms used included “compressive strength”, “elastic modulus” “flexural strength”, “fracture strength” and “fracture resistance”. The language filter was English. Of the initial 205 articles identified, 49 were included in this review, of which 29 were on SH, 21 on CH/MTA, and 1 relating to both. Many in vitro studies indicated a strong link between reduced mechanical properties of roots of teeth or radicular dentine treated with SH, and when sealers or root fillings with CH- or MTA-based materials were placed in contact with roots or radicular dentine. Recent literature indicates that the association between reduced mechanical properties and alkaline sealers and/or root-filling materials is not as straightforward as previously assumed, and requires further investigation using more valid experimental models.
Characterization and Bone Response of Carbonate-Containing Apatite-Coated Titanium Implants Using an Aqueous Spray Coating
Authors: Yagi Ryo , Mochizuki Chihiro , Sato Mitsunobu , Toyama Takeshi , Hirota Masatsugu , Hayakawa Tohru , Ohkubo Chikahiro
Published: December 2017

We performed thin carbonate-containing apatite (CA) coating on titanium (Ti) by an aqueous spray coating (ASC) method that consisted of a Ca-CO3-PO4 complex. Two different CA coatings were produced by two different spray amounts and were heat-treated after spraying. We evaluated three-dimensional structures, adhesiveness to Ti, and durability of the CA film. In addition, we performed immersion experiments in simulated body fluid (SBF), and bone responses were evaluated after implantation into a femoral bone defect in rats. The bonding ability of ASC-coated implant into the bone was examined by push-in tests. Unique network structures with small particles were identified on CA coatings. Although heat treatment produced no significant difference in surface morphology, scratch tests revealed that heat treatment improved the adhesion of CA coatings to Ti. Crystal formation progressed on CA-coated specimens, and the sample placement direction influenced crystal formation and growth in SBF immersion. Animal implantation experiments revealed significantly greater bone-to-implant contact ratio and bone mass in both cortical and bone marrow, respectively, four weeks after implantation. Push-in tests suggested that the bonding of the CA coating to Ti is clinically acceptable. Therefore, we conclude that CA coating to Ti by the ASC method would be possible for clinical applications, including dentistry.
Pediatric Thermal Burns and Treatment: A Review of Progress and Future Prospects
Authors: Mathias Elton , Srinivas Murthy Madhu
Published: December 2017

Burn injuries are a devastating critical care problem. In children, burns continue to be a major epidemiologic problem around the globe resulting in significant morbidity and death. Apparently, treating these burn injuries in children and adults remains similar, but there are significant physiological and psychological differences. The dermal layer of the skin is generally thinner in neonates, infants, and children than in adults. Enhanced evaporative loss and need for isotonic fluids increases the risk of hypothermia in the pediatric population. The pain management of the children with major burns challenges the skills of the personnel of every unit. Managing these wounds requires intensive therapeutic treatment for multi-organ dysfunction, and surgical treatment to prevent sepsis and other complications that further delay wound closure. Alternatives to the practice of donor site harvest and autografting for the treatment of severe burns and other complex skin defects are urgently needed for both adult and pediatric populations. This review article focuses on thermal burn pathophysiology and pain management and provides an overview of currently approved products used for the treatment of pediatric burn wounds. A new promising approach has been presented as a first-line therapy in the treatment of burns to reduce surgical autografting in pediatric patients.
Extraction Optimization of Flavonoids from Hypericum formosanum and Matrix Metalloproteinase-1 Inhibitory Activity
Authors: Huang Ho-Shin , Liaw Ean-Tun
Published: December 2017

Hypericum formosanum is a valuable herb in Taiwan. In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction of flavonoids from Hypericum formosanum. A central composite design with three variables (ethanol concentration, extraction time, and extraction temperature) was applied. Experimental results were fitted to the second order polynomial model and one-way analysis of variance was used to determine the goodness of fit of the model and the optimal conditions for responses. The optimal conditions for the maximum extraction yield of total flavonoid content (101.1 mg/g) using ultrasound-assisted extraction were ethanol concentration, 73.5%; extraction time, 38.3 min; and extraction temperature, 62.5 °C. The predicted result was consistent with the experimental result obtained under optimal extraction conditions. Hyperoside, astilbin, quercitrin, and quercetin from Hypericum formosanum extract (HFE) were identified by Ultra performance liquid chromatography-diode array detector-mass (UPLC-DAD-MS). HFE significantly reduced matrix metalloproteinase-1 protein expression in human skin keratinocyte cells, induced by advanced glycation end products.
Five- and Six-Membered Nitrogen-Containing Compounds as Selective Carbonic Anhydrase Activators
Authors: Mollica Adriano , Macedonio Giorgia , Stefanucci Azzurra , Carradori Simone , Akdemir Atilla , Angeli Andrea , Supuran Claudiu
Published: December 2017

It has been proven that specific isoforms of human carbonic anhydrase (hCA) are able to fine-tune physiological pathways connected to signal processing, and that decreased CAs expression negatively influences cognition, leading to mental retardation, Alzheimer’s disease, and aging-related cognitive dysfunctions. For this reason, a small library of natural and synthetic nitrogen containing cyclic derivatives was assayed as activators of four human isoforms of carbonic anhydrase (hCA I, II, IV and VII). Most of the compounds activated hCA I, IV and VII in the micromolar range, with KAs ranging between 3.46 and 80.5 μM, whereas they were not active towards hCA II (KAs > 100 μM). Two natural compounds, namely l-(+)-ergothioneine (1) and melatonin (2), displayed KAs towards hCA VII in the nanomolar range after evaluation by a CO2 hydration method in vitro, showing a rather efficient and selective activation profile with respect to histamine, used as a reference compound. Corroborated with the above in vitro findings, a molecular modelling in silico approach has been performed to correlate these biological data, and to elucidate the binding interaction of these activators within the enzyme active site.
Novel Effective Small-Molecule Antibacterials against Enterococcus Strains
Authors: Ashraf Kerolos , Yasrebi Kaveh , Hertlein Tobias , Ohlsen Knut , Lalk Michael , Hilgeroth Andreas
Published: December 2017

Enterococcus species cause increasing numbers of infections in hospitals. They contribute to the increasing mortality rates, mostly in patients with comorbidities, who suffer from severe diseases. Enterococcus resistances against most antibiotics have been described, including novel antibiotics. Therefore, there is an ongoing demand for novel types of antibiotics that may overcome bacterial resistances. We discovered a novel class of antibiotics resulting from a simple one-pot reaction of indole and o-phthaldialdehyde. Differently substituted indolyl benzocarbazoles were yielded. Both the indole substitution and the positioning at the molecular scaffold influence the antibacterial activity towards the various strains of Enterococcus species with the highest relevance to nosocomial infections. Structure-activity relationships are discussed, and the first lead compounds were identified as also being effective in the case of a vancomycin resistance.
The Diverse Range of Possible Cell Membrane Interactions with Substrates: Drug Delivery, Interfaces and Mobility
Authors: Jang Hyun-Sook
Published: December 2017

The cell membrane has gained significant attention as a platform for the development of bio-inspired nanodevices due to its immune-evasive functionalities and copious bio-analogs. This review will examine several uses of cell membranes such as (i) therapeutic delivery carriers with or without substrates (i.e., nanoparticles and artificial polymers) that have enhanced efficiency regarding copious cargo loading and controlled release, (ii) exploiting nano-bio interfaces in membrane-coated particles from the macro- to the nanoscales, which would help resolve the biomedical issues involved in biological interfacing in the body, and (iii) its effects on the mobility of bio-moieties such as lipids and/or proteins in cell membranes, as discussed from a biophysical perspective. We anticipate that this review will influence both the development of novel anti-phagocytic delivery cargo and address biophysical problems in soft and complex cell membrane.
Effects of Bacterial Fermentation on the Biochemical Constituents and Antioxidant Potential of Fermented and Unfermented Soybeans Using Probiotic Bacillus subtilis (KCTC 13241)
Authors: Ali Muhammad , Kim Il-Doo , Bilal Saqib , Shahzad Raheem , Saeed Muhammad , Adhikari Bishnu , Nabi Rizwana , Kyo Jeong , Shin Dong-Hyun
Published: December 2017

Fermented soybeans, cheonggukjang (CKJ), are considered to be more wholesome than soybeans in Korea. To select the best soybean cultivar for making functional CKJ, a comparison was made between the biological activities of four soybean cultivars in their unfermented soybean (UFS) and CKJ states. Changes in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, superoxide dismutase (SOD)-like activity, total phenolic compounds, total amino acids, and isoflavones were investigated. The levels of DPPH, ABTS, SOD-like activity, and total phenolic compounds increased in CKJ among all cultivars. The isoflavone aglycone and total amino acids showed the highest amount in CKJ prepared from soybean cultivar Aga 3. These results suggest that the improved antioxidant activity of CKJ in all cultivars might occur because of the higher levels of aglycones and total phenolic compounds achieved during fermentation. Moreover, CKJ prepared from soybean cultivar Aga 3 showed higher antioxidant activity than the other cultivars and so can be considered for the commercial production of functional foods in the future.
New Three-Dimensional Porous Electrode Concept: Vertically-Aligned Carbon Nanotubes Directly Grown on Embroidered Copper Structures
Authors: Aguiló-Aguayo Noemí , Amade Roger , Hussain Shahzad , Bertran Enric , Bechtold Thomas
Published: December 2017

New three-dimensional (3D) porous electrode concepts are required to overcome limitations in Li-ion batteries in terms of morphology (e.g., shapes, dimensions), mechanical stability (e.g., flexibility, high electroactive mass loadings), and electrochemical performance (e.g., low volumetric energy densities and rate capabilities). Here a new electrode concept is introduced based on the direct growth of vertically-aligned carbon nanotubes (VA-CNTs) on embroidered Cu current collectors. The direct growth of VA-CNTs was achieved by plasma-enhanced chemical vapor deposition (PECVD), and there was no application of any post-treatment or cleaning procedure. The electrochemical behavior of the as-grown VA-CNTs was analyzed by charge/discharge cycles at different specific currents and with electrochemical impedance spectroscopy (EIS) measurements. The results were compared with values found in the literature. The as-grown VA-CNTs exhibit higher specific capacities than graphite and pristine VA-CNTs found in the literature. This together with the possibilities that the Cu embroidered structures offer in terms of specific surface area, total surface area, and designs provide a breakthrough in new 3D electrode concepts.
A Metagenomic and in Silico Functional Prediction of Gut Microbiota Profiles May Concur in Discovering New Cystic Fibrosis Patient-Targeted Probiotics
Authors: Vernocchi Pamela , Del Chierico Federica , Quagliariello Andrea , Ercolini Danilo , Lucidi Vincenzina , Putignani Lorenza
Published: December 2017

Cystic fibrosis (CF) is a life-limiting hereditary disorder that results in aberrant mucosa in the lungs and digestive tract, chronic respiratory infections, chronic inflammation, and the need for repeated antibiotic treatments. Probiotics have been demonstrated to improve the quality of life of CF patients. We investigated the distribution of gut microbiota (GM) bacteria to identify new potential probiotics for CF patients on the basis of GM patterns. Fecal samples of 28 CF patients and 31 healthy controls (HC) were collected and analyzed by 16S rRNA-based pyrosequencing analysis of GM, to produce CF-HC paired maps of the distribution of operational taxonomic units (OTUs), and by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) for Kyoto Encyclopedia of Genes and Genomes (KEGG) biomarker prediction. The maps were scanned to highlight the distribution of bacteria commonly claimed as probiotics, such as bifidobacteria and lactobacilli, and of butyrate-producing colon bacteria, such as Eubacterium spp. and Faecalibacterium prausnitzii. The analyses highlighted 24 OTUs eligible as putative probiotics. Eleven and nine species were prevalently associated with the GM of CF and HC subjects, respectively. Their KEGG prediction provided differential CF and HC pathways, indeed associated with health-promoting biochemical activities in the latter case. GM profiling and KEGG biomarkers concurred in the evaluation of nine bacterial species as novel putative probiotics that could be investigated for the nutritional management of CF patients.
Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
Authors: Miró Lluïsa , Garcia-Just Alba , Amat Concepció , Polo Javier , Moretó Miquel , Pérez-Bosque Anna
Published: December 2017

Increased life expectancy has promoted research on healthy aging. Aging is accompanied by increased non-specific immune activation (inflammaging) which favors the appearance of several disorders. Here, we study whether dietary supplementation with spray-dried animal plasma (SDP), which has been shown to reduce the activation of gut-associated lymphoid tissue (GALT) in rodents challenged by S. aureus enterotoxin B (SEB), and can also prevent the effects of aging on immune system homeostasis. We first characterized GALT in a mouse model of accelerated senescence (SAMP8) at different ages (compared to mice resistant to accelerated senescence; SAMR1). Second, we analyzed the SDP effects on GALT response to an SEB challenge in SAMP8 mice. In GALT characterization, aging increased the cell number and the percentage of activated Th lymphocytes in mesenteric lymph nodes and Peyer’s patches (all, p < 0.05), as well as the expression of IL-6 and TNF-α in intestinal mucosa (both, p < 0.05). With respect to GALT response to the SEB challenge, young mice showed increased expression of intestinal IL-6 and TNF-α, as well as lymphocyte recruitment and activation (all, p < 0.05). However, the immune response of senescent mice to the SEB challenge was weak, since SEB did not change cell recruitment or the percentage of activated Th lymphocytes. Mice supplemented with SDP showed improved capacity to respond to the SEB challenge, similar to the response of the young mice. These results indicate that senescent mice have an impaired mucosal immune response characterized by unspecific GALT activation and a weak specific immune response. SDP supplementation reduces non-specific basal immune activation, allowing for the generation of specific responses.
Catalyst Influence on Undesired Side Reactions in the Polycondensation of Fully Bio-Based Polyester Itaconates
Authors: Schoon Ina , Kluge Marcel , Eschig Steven , Robert Tobias
Published: December 2017

Bio-based unsaturated polyester resins derived from itaconic acid can be an alternative to established resins of this type in the field of radical-curing resins. However, one of the challenges of these polyester itaconates is the somewhat more elaborate synthetic process, especially under polycondensation conditions used on an industrial scale. The α,β-unsaturated double bond of the itaconic acid is prone to side reactions that can lead to the gelation of the polyester resin under standard conditions. This is especially true when bio-based diols such as 1,3-propanediol or 1,4-butanediol are used to obtain resins that are 100% derived from renewable resources. It was observed in earlier studies that high amounts of these aliphatic diols in the polyester lead to low conversion and gelation of the resins. In this work, a catalytic study using different diols was performed in order to elucidate the reasons for this behavior. It was shown that the choice of catalyst has a crucial influence on the side reactions occurring during the polycondensation reactions. In addition, the side reactions taking place were identified and suppressed. These results will allow for the synthesis of polyester itaconates on a larger scale, setting the stage for their industrial application.
Experimental Investigation of the Magnetorheological Behavior of PDMS Elastomer Reinforced with Iron Micro/Nanoparticles
Authors: Palacios-Pineda Luis , Perales-Martinez Imperio , Lozano-Sanchez Luis , Martínez-Romero Oscar , Puente-Córdova Jesús , Segura-Cárdenas Emmanuel , Elías-Zúñiga Alex
Published: December 2017

The aim of this article focuses on identifying how the addition of iron micro- and nanoparticles influences the physical properties of magnetorheological composite materials developed with a polydimethylsiloxane (PDMS) matrix with different contents of silicone oil used as additive. A number of characterization techniques have been performed in order to fully characterize the samples, such as cyclic and uniaxial extension, rheology, swelling, Vibrating sample magnetometer (VSM), X-ray Diffraction (XRD), Scanning electron microscopy (SEM), Fourier-Transform Infrared (FTIR), X-ray photoelectronic spectroscopy (XPS) and Thermogravimetric analysis (TGA). The comparison between two matrices with different shore hardnesses and their mechanical and chemical properties are elucidated by swelling and tensile tests. In fact, swelling tests showed that higher crosslink density leads to increasing elongation at break and tensile strength values for the composite materials. The best mechanical performance in the magnetorheological material was observed for those samples manufactured using a higher silicone oil content in a hard polymeric matrix. Furthermore, it has been found that the magnetic properties are enhanced when nanoparticles are used as fillers instead of microparticles.
The Effects of Forest Area Changes on Extreme Temperature Indexes between the 1900s and 2010s in Heilongjiang Province, China
Authors: Zhang Lijuan , Pan Tao , Zhang Hongwen , Li Xiaxiang , Jiang Lanqi
Published: December 2017

Land use and land cover changes (LUCC) are thought to be amongst the most important impacts exerted by humans on climate. However, relatively little research has been carried out so far on the effects of LUCC on extreme climate change other than on regional temperatures and precipitation. In this paper, we apply a regional weather research and forecasting (WRF) climate model using LUCC data from Heilongjiang Province, that was collected between the 1900s and 2010s, to explore how changes in forest cover influence extreme temperature indexes. Our selection of extreme high, low, and daily temperature indexes for analysis in this study enables the calculation of a five-year numerical integration trail with changing forest space. Results indicate that the total forested area of Heilongjiang Province decreased by 28% between the 1900s and 2010s. This decrease is most marked in the western, southwestern, and northeastern parts of the province. Our results also reveal a remarkable correlation between change in forested area and extreme high and low temperature indexes. Further analysis enabled us to determine that the key factor explaining increases in extreme high temperature indexes (i.e., calculated using the number of warm days, warm nights, as well as tropical nights, and summer days) is decreasing forest area; data also showed that this factor caused a decrease in extreme low temperature indexes (i.e., calculated using the number of cold days and cold nights, as well as frost days, and ice days) and an increase in the maximum value of daily minimum temperature. Spatial data demonstrated that there is a significant correlation between forest-to-farmland conversion and extreme temperature indexes throughout most of our study period. Spatial data demonstrated that there is a significant correlation between forest-to-farmland conversion and extreme temperature indexes throughout most of our study period. Positive correlations are also present between decreasing forest area, the more frequent occurrence of extreme high temperature events, and a rise in the maximum value of daily minimum temperature. At the same time, we found clear negative correlations between decreasing forest area and less frequent occurrence of extreme low temperature events.
Using GRACE Satellite Gravimetry for Assessing Large-Scale Hydrologic Extremes
Authors: Sun Alexander , Scanlon Bridget , AghaKouchak Amir , Zhang Zizhan
Published: December 2017

Global assessment of the spatiotemporal variability in terrestrial total water storage anomalies (TWSA) in response to hydrologic extremes is critical for water resources management. Using TWSA derived from the gravity recovery and climate experiment (GRACE) satellites, this study systematically assessed the skill of the TWSA-climatology (TC) approach and breakpoint (BP) detection method for identifying large-scale hydrologic extremes. The TC approach calculates standardized anomalies by using the mean and standard deviation of the GRACE TWSA corresponding to each month. In the BP detection method, the empirical mode decomposition (EMD) is first applied to identify the mean return period of TWSA extremes, and then a statistical procedure is used to identify the actual occurrence times of abrupt changes (i.e., BPs) in TWSA. Both detection methods were demonstrated on basin-averaged TWSA time series for the world’s 35 largest river basins. A nonlinear event coincidence analysis measure was applied to cross-examine abrupt changes detected by these methods with those detected by the Standardized Precipitation Index (SPI). Results show that our EMD-assisted BP procedure is a promising tool for identifying hydrologic extremes using GRACE TWSA data. Abrupt changes detected by the BP method coincide well with those of the SPI anomalies and with documented hydrologic extreme events. Event timings obtained by the TC method were ambiguous for a number of river basins studied, probably because the GRACE data length is too short to derive long-term climatology at this time. The BP approach demonstrates a robust wet-dry anomaly detection capability, which will be important for applications with the upcoming GRACE Follow-On mission.
Intraspecific Differences in Spectral Reflectance Curves as Indicators of Reduced Vitality in High-Arctic Plants
Authors: Zagajewski Bogdan , Tømmervik Hans , Bjerke Jarle , Raczko Edwin , Bochenek Zbigniew , Kłos Andrzej , Jarocińska Anna , Lavender Samantha , Ziółkowski Dariusz
Published: December 2017

Remote sensing is a suitable candidate for monitoring rapid changes in Polar regions, offering high-resolution spectral, spatial and radiometric data. This paper focuses on the spectral properties of dominant plant species acquired during the first week of August 2015. Twenty-eight plots were selected, which could easily be identified in the field as well as on RapidEye satellite imagery. Spectral measurements of individual species were acquired, and heavy metal contamination stress factors were measured contemporaneously. As a result, a unique spectral library of dominant plant species, heavy metal concentrations and damage ratios were achieved with an indication that species-specific changes due to environmental conditions can best be differentiated in the 1401–2400 nm spectral region. Two key arctic tundra species, Cassiope tetragona and Dryas octopetala, exhibited significant differences in this spectral region that were linked to a changing health status. Relationships between field and satellite measurements were comparable, e.g., the Red Edge Normalized Difference Vegetation Index (RENDVI) showed a strong and significant relationship (R2 = 0.82; p = 0.036) for the species Dryas octopetala. Cadmium and Lead were below detection levels while manganese, copper and zinc acquired near Longyearbyen were at concentrations comparable to other places in Svalbard. There were high levels of nickel near Longyearbyen (0.014 mg/g), while it was low (0.004 mg/g) elsewhere.
Evaluation of the SBAS InSAR Service of the European Space Agency’s Geohazard Exploitation Platform (GEP)
Authors: Galve Jorge Pedro , Pérez-Peña José Vicente , Azañón José Miguel , Closson Damien , Caló Fabiana , Reyes-Carmona Cristina , Jabaloy Antonio , Ruano Patricia , Mateos Rosa María , Notti Davide , Herrera Gerardo , Béjar-Pizarro Marta , Monserrat Oriol , Bally Philippe
Published: December 2017

The analysis of remote sensing data to assess geohazards is being improved by web-based platforms and collaborative projects, such as the Geohazard Exploitation Platform (GEP) of the European Space Agency (ESA). This paper presents the evaluation of a surface velocity map that is generated by this platform. The map was produced through an unsupervised Multi-temporal InSAR (MTI) analysis applying the Parallel-SBAS (P-SBAS) algorithm to 25 ENVISAT satellite images from the South of Spain that were acquired between 2003 and 2008. This analysis was carried out using a service implemented in the GEP called “SBAS InSAR”. Thanks to the map that was generated by the SBAS InSAR service, we identified processes not documented so far; provided new monitoring data in places affected by known ground instabilities; defined the area affected by these instabilities; and, studied a case where GEP could have been able to help in the forecast of a slope movement reactivation. This amply demonstrates the reliability and usefulness of the GEP, and shows how web-based platforms may enhance the capacity to identify, monitor, and assess hazards that are associated to geological processes.
Synergic Use of Sentinel-1 and Sentinel-2 Images for Operational Soil Moisture Mapping at High Spatial Resolution over Agricultural Areas
Authors: El Hajj Mohammad , Baghdadi Nicolas , Zribi Mehrez , Bazzi Hassan
Published: December 2017

Soil moisture mapping at a high spatial resolution is very important for several applications in hydrology, agriculture and risk assessment. With the arrival of the free Sentinel data at high spatial and temporal resolutions, the development of soil moisture products that can better meet the needs of users is now possible. In this context, the main objective of the present paper is to develop an operational approach for soil moisture mapping in agricultural areas at a high spatial resolution over bare soils, as well as soils with vegetation cover. The developed approach is based on the synergic use of radar and optical data. A neural network technique was used to develop an operational method for soil moisture estimates. Three inversion SAR (Synthetic Aperture Radar) configurations were tested: (1) VV polarization; (2) VH polarization; and (3) both VV and VH polarization, all in addition to the NDVI information extracted from optical images. Neural networks were developed and validated using synthetic and real databases. The results showed that the use of a priori information on the soil moisture condition increases the precision of the soil moisture estimates. The results showed that VV alone provides better accuracy on the soil moisture estimates than VH alone. In addition, the use of both VV and VH provides similar results, compared to VV alone. In conclusion, the soil moisture could be estimated in agricultural areas with an accuracy of approximately 5 vol % (volumetric unit expressed in percent). Better results were obtained for soil with a moderate surface roughness (for root mean surface height between 1 and 3 cm). The developed approach could be applied for agricultural plots with an NDVI lower than 0.75.
A New Laccase Based Biosensor for Tartrazine
Authors: Mazlan Siti , Lee Yook , Hanifah Sharina
Published: December 2017

Laccase enzyme, a commonly used enzyme for the construction of biosensors for phenolic compounds was used for the first time to develop a new biosensor for the determination of the azo-dye tartrazine. The electrochemical biosensor was based on the immobilization of laccase on functionalized methacrylate-acrylate microspheres. The biosensor membrane is a composite of the laccase conjugated microspheres and gold nanoparticles (AuNPs) coated on a carbon-paste screen-printed electrode. The reaction involving tartrazine can be catalyzed by laccase enzyme, where the current change was measured by differential pulse voltammetry (DPV) at 1.1 V. The anodic peak current was linear within the tartrazine concentration range of 0.2 to 14 μM (R2 = 0.979) and the detection limit was 0.04 μM. Common food ingredients or additives such as glucose, sucrose, ascorbic acid, phenol and sunset yellow did not interfere with the biosensor response. Furthermore, the biosensor response was stable up to 30 days of storage period at 4 °C. Foods and beverage were used as real samples for the biosensor validation. The biosensor response to tartrazine showed no significant difference with a standard HPLC method for tartrazine analysis.