bluuebunny/crossref_metadata_2025 huggingface dataset:
Microglial activating transcription factor 3 upregulation: An indirect target to attenuate inflammation in the nervous system
Authors: Holland Seth D. , Ramer Matt S.
Published: March 2023

Activating Transcription Factor 3 (ATF3) is upregulated in reaction to several cellular stressors found in a wide range of pathological conditions to coordinate a transcriptional response. ATF3 was first implicated in the transcriptional reaction to axotomy when its massive upregulation was measured in sensory and motor neuron cell bodies following peripheral nerve injury. It has since been shown to be critical for successful axon regeneration in the peripheral nervous system and a promising target to mitigate regenerative failure in the central nervous system. However, much of the research to date has focused on ATF3’s function in neurons, leaving the expression, function, and therapeutic potential of ATF3 in glia largely unexplored. In the immunology literature ATF3 is seen as a master regulator of the innate immune system. Specifically, in macrophages following pathogen or damage associated molecular pattern receptor activation and subsequent cytokine release, ATF3 upregulation abrogates the inflammatory response. Importantly, ATF3 upregulation is not exclusively due to cellular stress exposure but has been achieved by the administration of several small molecules. In the central nervous system, microglia represent the resident macrophage population and are therefore of immediate interest with respect to ATF3 induction. It is our perspective that the potential of inducing ATF3 expression to dampen inflammatory microglial phenotype represents an unexplored therapeutic target and may have synergistic benefits when paired with concomitant neuronal ATF3 upregulation. This would be of particular benefit in pathologies that involve both detrimental inflammation and neuronal damage including spinal cord injury, multiple sclerosis, and stroke.
No evidence of a genetic causal relationship between ankylosing spondylitis and iron homeostasis: A two-sample Mendelian randomization study
Authors: Yang Mingyi , Yu Hui , Xu Ke , Xie Jiale , Zheng Haishi , Feng Ruoyang , Wang Jiachen , Xu Peng
Published: March 2023

Background

Ankylosing spondylitis (AS) is an immune-mediated chronic inflammatory disease that leads to bone hyperplasia and spinal ankylosis. Iron homeostasis plays a very important role in the inflammatory response and is closely related to the pathogenesis of AS. This study aimed to use large-scale genome-wide association study (GWAS) summary data to study the genetic causal relationship between AS and iron homeostasis using Mendelian randomization (MR).

Methods

Genome-wide association study summary data of AS and iron homeostasis-related indicators were obtained from the FinnGen consortium and the DeCODE genetics database, respectively. We used four iron homeostasis-related indicators: ferritin, serum iron, total iron binding capacity (TIBC), and transferrin saturation (TSAT) for two-sample MR analyses to test for genetic causal association with AS using the “TwoSampleMR” package of the R software (version 4.1.2). The random-effects inverse variance weighted (IVW) method was the main analysis method used for MR. We examined the MR analysis results for heterogeneity, horizontal pleiotropy, and possible outliers. In addition, we confirmed the robustness of the MR analysis by testing whether the results were affected by a single SNP and whether they followed a normal distribution.

Results

The random-effects IVW results showed that ferritin [p = 0.225, OR 95% confidence interval (CI) = 0.836 (0.627–1.116)], serum iron [p = 0.714, OR 95% CI = 0.948 (0.714–1.260)], TIBC [p = 0.380, OR 95% CI = 0.917 (0.755–1.113)], and TSAT [p = 0.674, OR 95% CI = 0.942 (0.713–1.244)] have no genetic causal relationship with AS. We detected no heterogeneity，horizontal pleiotropy and possible outliers in our MR analysis (p > 0.05). In addition, our MR analysis results were not affected by a single SNP, and were normally distributed.

Conclusion

Our study did not detect a genetic causal relationship between AS and iron homeostasis. Nonetheless, this does not rule out a relationship between the two at other mechanistic levels.
Amino acids in hematologic malignancies: Current status and future perspective
Authors: Wang Mengyao , Zhao Ailin , Li Meng , Niu Ting
Published: March 2023

In recent years, growing emphasis has been placed on amino acids and their role in hematologic malignancies. Cancer cell metabolism is altered during tumorigenesis and development to meet expanding energetic and biosynthetic demands. Amino acids not only act as energy-supplying substances, but also play a vital role via regulating key signaling pathways, modulating epigenetic factors and remodeling tumor microenvironment. Targeting amino acids may be an effective therapeutic approach to address the current therapeutic challenges. Here, we provide an updated overview of mechanisms by which amino acids facilitate tumor development and therapy resistance. We also summarize novel therapies targeting amino acids, focusing on recent advances in basic research and their potential clinical implications.
Lycopene prevents non-alcoholic fatty liver disease through regulating hepatic NF-κB/NLRP3 inflammasome pathway and intestinal microbiota in mice fed with high-fat and high-fructose diet
Authors: Gao Xiang , Zhao Xia , Liu Min , Zhao Huimin , Sun Yongye
Published: March 2023

Lycopene (LY) belongs to carotenoids and is abundant in red fruits and vegetables. Several previous studies suggested that LY is beneficial for ameliorating non-alcoholic fatty liver disease (NAFLD), while the potential mechanisms are unclear. The present study aimed to clarify the potential mechanisms of LY in preventing NAFLD via exploring the hepatic NF-κB/NLRP3 inflammasome pathway and intestinal microbiota composition in high-fat and high-fructose diet (HFFD)-fed mice. Fifty eight-week-old male C57BL/6J mice were randomly assigned into 5 groups: Normal control group (NC); HFFD group; HFFD with low dose of lycopene group (LLY, 20 mg/kg/d); HFFD with high dose of lycopene group (HLY, 60 mg/kg/d) and HFFD with resveratrol group (RSV, 50 mg/kg/d, positive control). After 8 weeks, feces were collected and the 12 h fasted mice were sacrificed to acquire tissues and blood for parameters measurement. The results showed that the mice in LLY, HLY and RSV groups had significantly lower body weight gain, weight of white adipose tissue, serum levels of high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), lipopolysaccharide (LPS), alanine aminotransferase (ALT), and hepatic concentrations of triglyceride (TG) and interleukin-6 (IL-6) than that in the HFFD group (p < 0.05). HLY and RSV groups also displayed lower serum levels of TG, total cholesterol (TC) and hepatic levels of tumor necrosis factor-α (TNF-α) than the HFFD group (p < 0.05). Liver protein expressions of NLRP3, Pro-Caspase-1, Caspase-1 and NF-κB were lower in the LLY, HLY and RSV groups than those in the HFFD group (p < 0.05). The feces of LY -treated mice had higher relative levels of SCFAs producing bacteria Allobaculum and lower destructive bacteria, including Firmicutes, Lachnospiraceae_NK4A136_group, Desulfovibrio, and Alistipes over the HFFD group (p < 0.05). RSV group also displayed lower fecal levels of Lachnospiraceae_NK4A136_group, Desulfovibrio, and Alistipes than the HFFD group (p < 0.05). In conclusion, LY might prevent NAFLD by suppressing hepatic NF-κB/NLRP3 inflammasome pathway and attenuating gut microbiota dysbiosis.
Effects of low-GI biscuits as pre-loads or mid-meal snacks on post-prandial glycemic excursions in women with recent gestational diabetes: A protocol for a randomized crossover trial and an extended tailored intervention
Authors: Li Chunrong , Gao Yan , Luo Tongyong , Qin Shiji , Yao Xue , Wen Ye , Wang Xue , Zhang Jing , Zhong Qiong , Shi Hao , Liu Jing
Published: March 2023

Background

Increased post-prandial glycemic excursions contribute to the development of diabetes and have been observed in women with recent gestational diabetes mellitus (GDM) and with normal glucose tolerance at post-partum. As a convenient meal replacement, low-GI biscuits are helpful for improving glycemic excursions in patients with type 2 diabetes. However, it is unknown whether low-GI biscuits as pre-loads or mid-meal snacks have a better effect in diminishing post-prandial glycemic excursions from the individual level in women with recent GDM. Therefore, the aim of this trial is to tailor a better dietary strategy utilizing low-GI biscuits (Fitmeal) to improve post-prandial glycemic excursions through within-subject comparison in such a population and observe the long-term effect of a tailored dietary approach in glycemic control.

Methods

We have designed a two-phase trial including a randomized, crossover, non-blinded trial in the first phase, followed by a 4-week tailored intervention in the second phase. A total of 52 post-partum women with recent GDM will be allocated into four meal plans: (1) Fitmeal pre-load 30 min before standard lunch meal (P+L), (2) Fitmeal as a mid-meal snack 2 h before standard lunch meal (S+L), (3) isocaloric standard control with co-ingestion of Fitmeal and standard lunch meal (CL) at the same time, and (4) placebo control with 200 ml of water taken 30 min before standard lunch meal (W + L), on four consecutive days. Acute post-prandial glycemic response (PGR) measured by continuous glucose monitoring (CGM) will be compared among the four meals. In the second phase, all participants will receive a 4-week tailored intervention using Fitmeal as pre-loads or mid-meal snacks based on within-subject PGR results from the first phase. Glycemic metrics, dietary behaviors, and psychosocial factors (e.g., quality of life, self-efficacy, perceived stress, and depression) will be examined at baseline and end-point.

Discussion

This trial is expected to optimize the use of low-GI biscuits as pre-loads or mid-meal snacks in improving individual post-prandial glycemic excursions among women with recent GDM. Furthermore, the findings of this study will provide novel information on how to deliver an effective dietary intervention at the individual level and guide future clinical practice of medical nutrition therapy for diabetes prevention.

Trial registration number

Chinese clinical trial registry, ChiCTR2200060923.
Association between the prudent dietary pattern and blood pressure in Chinese adults is partially mediated by body composition
Authors: Chen Mengxue , Xu Yujie , Wang Xiaoyu , Shan Shufang , Cheng Guo
Published: March 2023

High blood pressure or hypertension is one of the major risks of cardiovascular disease, which is the leading cause of death in China. This study aimed to assess the relationship between dietary patterns and blood pressure among Chinese adults. Using factor analysis of 66-item food frequency questionnaire to identify dietary patterns. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured according to standardized guidelines. Multivariate linear regressions were performed in 6849 Chinese adults (46.5% female) aged 21–70 years considering sociodemographic characteristics, lifestyle behaviors, and anthropometry data. The vegetable-rich pattern, animal-food pattern, and prudent dietary pattern were identified. After adjustment for potential confounders including age, gender, alcohol consumption, smoking status, energy intake, and physical activity, only prudent dietary pattern was negatively related to SBP (β = −2.30, p for trend =0.0003) and DBP (β = −1.44, p for trend =0.0006). Body mass index, waist circumstance and body fat percentage explained, respectively, 42.5%/47.8, 14.8%/17.6 and 26.0%/29.1% of the association between prudent pattern and SBP/DBP in mediation analysis. There were no association were observed between other dietary patterns and blood pressure. In conclusion, Prudent dietary pattern was associated with lower SBP and DBP among Southwest Chinese and this association was partially explained by body composition.
The lipid-lowering effects of fenugreek gum, hawthorn pectin, and burdock inulin
Authors: Wang Yi , Zheng Yu , Liu Yi , Shan Guoshun , Zhang Baojie , Cai Qian , Lou Jiayue , Qu Yang
Published: March 2023

Objective

The present study aimed to investigate the lipid-lowering effects and mechanisms of fenugreek gum (FG), hawthorn pectin (HP), and burdock inulin (BI) on high-fat diet (HFD)-induced hyperlipidemic rats.

Methods

In this study, high-fat diet (HFD) together with fat emulsion administration were used to establish hyperlipidemia model. The biochemical indices were assayed after administration of FG, HP, and BI. Their effects were evaluated by factor analysis. Alterations of gut microbiota and short chain fatty acids (SCFAs) in the cecal were assessed to illustrate the mechanism of lipid lowering.

Results

The supplementation of FG, HP, and BI on HFD-fed rats decreased the levels of serum lipid and reduced the HFD-related liver and testicle damage. In the scatter plot of factor analysis, HP and BI were closer to normal fat diet (NFD) group in restoring the severity of hyperlipidemia, while FG and HP enhanced the excretion of cholesterol and bile acids (BAs). The levels of total SCFAs, especially butyric acid reduced by HFD were increased by HP. The ratio of Firmicutes to Bacteroidetes increased by HFD was reduced by HP and BI. FG, HP, and BI enriched intestinal probiotics, which were related to bile acid excretion or lipid-lowering.

Conclusions

FG inhibited the absorption of cholesterol and enhanced the excretion of it, as well as increased the abundance of beneficial bacteria. While BI restored the imbalance of intestinal microbiota. HP enhanced the excretion of cholesterol and BAs, and restored the imbalance of intestinal microbiota. It was also utilized by intestinal microorganisms to yield SCFAs. This study suggested that FG, HP, and BI possessed the potential to be utilized as dietary supplements for obesity management.
Association of macronutrients intake distribution with osteoarthritis risk among adults in NHANES, 2013–2016
Authors: Peng Peng , Gao Shihua , Xiao Fangjun , He Mincong , Fang Weiuhua , Zhang Yunqi , Wei Qiushi
Published: March 2023

The association between dietary macronutrient distribution and the risk of OA remains unknown. We aimed to evaluate how dietary macronutrient distribution was correlated with the risk of OA in US adults. We performed a cross-sectional study consisting of 7,725 participants from National Health and Nutrition Examination Survey (NHANES) 2013–2016. Dietary macronutrient intake and OA status were assessed by using dietary recall method and self-reported questionnaire, respectively. We evaluated the association between dietary macronutrient distribution and the risk of OA using multivariate regression models. We conducted the isocaloric substitution analysis using the multivariate nutrient density method. Higher percentage of energy intake from fat was associated with higher risk of OA [OR = 1.05 (95% CI, 1.00, 1.09); P = 0.034]. No significant correlation was observed between the percentage of energy intake from carbohydrate or protein and risk of OA. Isocaloric substitution analysis revealed that only the substitution between fat and carbohydrate was significantly associated with the risk of OA [OR = 1.05 (95% CI, 1.003 to 1.09); P = 0.037]. Our findings suggested that a diet with low percentage of energy intake from fat may be beneficial in the prevention of OA. Further prospective cohort studies are needed to assess our results.
RAD-UNet: Research on an improved lung nodule semantic segmentation algorithm based on deep learning
Authors: Wu Zezhi , Li Xiaoshu , Zuo Jianhui
Published: March 2023

Objective

Due to the small proportion of target pixels in computed tomography (CT) images and the high similarity with the environment, convolutional neural network-based semantic segmentation models are difficult to develop by using deep learning. Extracting feature information often leads to under- or oversegmentation of lesions in CT images. In this paper, an improved convolutional neural network segmentation model known as RAD-UNet, which is based on the U-Net encoder-decoder architecture, is proposed and applied to lung nodular segmentation in CT images.

Method

The proposed RAD-UNet segmentation model includes several improved components: the U-Net encoder is replaced by a ResNet residual network module; an atrous spatial pyramid pooling module is added after the U-Net encoder; and the U-Net decoder is improved by introducing a cross-fusion feature module with channel and spatial attention.

Results

The segmentation model was applied to the LIDC dataset and a CT dataset collected by the Affiliated Hospital of Anhui Medical University. The experimental results show that compared with the existing SegNet [14] and U-Net [15] methods, the proposed model demonstrates better lung lesion segmentation performance. On the above two datasets, the mIoU reached 87.76% and 88.13%, and the F1-score reached 93.56% and 93.72%, respectively. Conclusion: The experimental results show that the improved RAD-UNet segmentation method achieves more accurate pixel-level segmentation in CT images of lung tumours and identifies lung nodules better than the SegNet [14] and U-Net [15] models. The problems of under- and oversegmentation that occur during segmentation are solved, effectively improving the image segmentation performance.
Personalized intrahepatic cholangiocarcinoma prognosis prediction using radiomics: Application and development trend
Authors: Chen Pengyu , Yang Zhenwei , Zhang Haofeng , Huang Guan , Li Qingshan , Ning Peigang , Yu Haibo
Published: March 2023

Radiomics was proposed by Lambin et al. in 2012 and since then there has been an explosion of related research. There has been significant interest in developing high-throughput methods that can automatically extract a large number of quantitative image features from medical images for better diagnostic or predictive performance. There have also been numerous radiomics investigations on intrahepatic cholangiocarcinoma in recent years, but no pertinent review materials are readily available. This work discusses the modeling analysis of radiomics for the prediction of lymph node metastasis, microvascular invasion, and early recurrence of intrahepatic cholangiocarcinoma, as well as the use of deep learning. This paper briefly reviews the current status of radiomics research to provide a reference for future studies.
The origins, roles and therapies of cancer associated fibroblast in liver cancer
Authors: Zulaziz Natasha , Chai San Jiun , Lim Kue Peng
Published: March 2023

Hepatocellular carcinoma (HCC) is the most common form of liver cancer. It is often preceded by chronic inflammation such as liver fibrosis and cirrhosis. Different cell types are believed to give rise to liver-specific cancer associated fibroblast (CAF), these include resident fibroblast, hepatic stellate cell, liver cancer cell, hepatic sinusoidal endothelial cell and mesenchymal stromal cell. The abundance of fibroblasts has contributed to the cancer progression, immune modulation and treatment resistance in HCC. In this review, we discussed the origins, subtypes and roles of cancer associated fibroblasts in HCC. Their specific roles in shaping the tumor microenvironment, facilitating cancer growth, and modulating different immune cell types to confer a permissive environment for cancer growth. CAF is now an attractive therapeutic target for cancer treatment, however specific therapeutic development in HCC is still lacking. Hence, we have included preclinical and clinical development of CAF-specific interventions for other cancer types in this review. However, most CAF-specific therapies have resulted in disappointing clinical outcomes, likely due to the difficulties in differentiating CAF from normal fibroblast. A thorough understanding of the characteristics and functionalities of CAF is warranted to further improve the therapeutic efficacy of anti-CAF therapies.
Number of positive lymph nodes affects outcomes in parotid adenoid cystic carcinoma
Authors: Han Feng
Published: March 2023

Objectives

Survival significance of the number of positive lymph nodes (LNs) in parotid adenoid cystic carcinoma (ACC) remains unknown; thus, this study aimed to determine the impact of the number of positive LNs on the prognosis of parotid ACC.

Methods

Patients with surgically treated parotid ACC were enrolled from the SEER database. The number of positive LNs was analyzed using three models (0 vs 1+, 0 vs 1 vs 2 vs 3 vs 4 vs 4 vs 5 vs 6+, 0/1 vs 2–4 vs 5+), its hazard ratios on disease specific survival (DSS) and overall survival (OS) were assessed using univariate and multivariate Cox analyses.

Results

A total of 1,689 patients were included. In all models, the number of positive LNs was independently related to DSS and OS, model 3 had the highest C-index for DSS [0.83 (95% CI: 0.81–0.85)] and OS [0.82 (95% CI: 0.80–0.84)]. Compared with the 0/1 positive LN group, the 2–4 positive LN group had an HR of 2.81 (95% CI: 1.73–4.56) for DSS and 2.36 (95% CI: 1.58–3.54) for OS. The 5+ LN group had an HR of 20.15 (95% CI: 7.50–54.18) for DSS and 14.20 (95% CI: 5.45–36.97) for OS. No overlap existed in the 95% CI of the HR.

Conclusions

The three prognostic categories based on the number of positive LNs (0/1 vs 2–4 vs 5+) could stratify the DSS and OS in parotid ACC without overlap.
Heterogeneous pattern of gene expression driven by TTN mutation is involved in the construction of a prognosis model of lung squamous cell carcinoma
Authors: Liu Zhao , Zhao Xiaowen , Wang Ruihong , Tang Xiangyue , Zhao Yuxiang , Zhong Guanghui , Peng Xin , Zhang Chunlin
Published: March 2023

Objective

To investigate the impact that TTN mutation had on the gene heterogeneity expression and prognosis in patients with lung adenocarcinoma.

Methods

In this study, the Cancer Genome Atlas (TCGA) dataset was used to analyze the TTN mutations in lung adenocarcinoma. Lung adenocarcinoma data was collected from the TCGA database, clinical information of patients was analyzed, and bioinformatics statistical methods were applied for mutation analysis and prognosis survival analysis. The results were verified using the GEO dataset.

Results

The incidence of TTN mutations in lung adenocarcinoma was found to be 73%, and it was related to the prognosis of lung adenocarcinoma. Ten genes were screened with significant contributions to prognosis. A prognosis model was constructed and verified by LASSO COX analysis in the TCGA and GEO datasets based on these ten beneficial factors. The independent prognostic factor H2BC9 for TTN mutation-driven gene heterogeneity expression was screened through multi-factor COX regression analysis.

Conclusion

Our data showed that the gene heterogeneity expression, which was driven by TTN mutations, prolonged the survival of lung adenocarcinoma patients and provided valuable clues for the prognosis of TTN gene mutations in lung adenocarcinoma.
Aberration in myeloid-derived pro-angiogenic cells in type-2 diabetes mellitus; implication for diabetic retinopathy?
Authors: Shariatzadeh Mahnaz , Binda Trishika R.R. , van Holten-Neelen Conny , ten Berge Josianne C. , Martinez Ciriano Jose P. , Wong King T. , Dik Willem A. , Leenen Pieter J.M.
Published: March 2023

Purpose

Diabetic retinopathy (DR) is a major microvascular complication of type 2 diabetes mellitus (T2DM). Myelomonocytic proangiogenic cells (PAC) have been implicated in DR pathogenesis, but their functional and developmental abnormalities are unclear. In this study we assessed PAC characteristics from healthy controls, T2DM patients with DR (DR) and without (NoDR) in order to determine the consequence of the diabetic condition on PAC phenotype and function, and whether these differ between DR and NoDR patients.

Methods

PAC were generated by culturing PBMC on fibronectin coating and then immunophenotyped using flow cytometry. Furthermore, cells were sorted based on CD14, CD105, and CD133 expression and added to an in vitro 3-D endothelial tubule formation assay, containing GFP-expressing human retinal endothelial cells (REC), pericytes, and pro-angiogenic growth factors. Tubule formation was quantified by fluorescence microscopy and image analysis. Moreover, sorted populations were analyzed for angiogenic mediator production using a multiplex assay.

Results

The expression of CD16, CD105 and CD31, but not CD133, was lower in PAC from T2DM patients with or without DR. Myeloid and non-myeloid T2DM-derived sorted populations increased REC angiogenesis in vitro as compared to control cultures. They also showed increased S100A8 secretion, decreased VEGF-A secretion, and similar levels of IL-8, HGF, and IL-3 as compared to healthy control (HC)-derived cell populations.

Conclusion

T2DM PAC are phenotypically and functionally altered compared to PAC from HC. Differences between DR and NoDR PAC are limited. We propose that impaired T2DM PAC provide inadequate vascular support and promote compensatory, albeit pathological, retinal neovascularization.
Time series analysis of non-invasive hemodynamic monitoring data in neonates with hypoxic-ischemic encephalopathy
Authors: Balog Vera , Vatai Barbara , Kovacs Kata , Szabo Attila J. , Szabo Miklos , Jermendy Agnes
Published: March 2023

Background and aims

Hemodynamic instability is common in neonates with hypoxic-ischemic encephalopathy (HIE) undergoing therapeutic hypothermia (TH). Rewarming is a critical period and non-invasive circulatory monitoring may help guide cardiovascular supportive therapy. The aim of the study was to provide a comprehensive analysis of cardiac function parameters during TH and its relation to neurodevelopmental outcome.

Methods

In a prospective, observational study, 26 neonates with moderate-severe HIE were enrolled, born between 2016 and 2019. A hemodynamic monitor based on electrical velocimetry (ICON, Osypka Medical GmbH, Berlin, Germany) was used. Heart rate (HR), stroke volume (SV), cardiac output (CO) data were recorded continuously throughout TH and rewarming. Neurological outcome was assessed at 2 years of age using the Bayley Scales of Infant Development II. edition. Favorable outcome was defined as >70 points on both the psychomotor and mental scales. Time-series analysis was used and features of cardiac function were described to perform logistic regression modeling for outcome prediction.

Results

Fourteen (54%) patients had favorable and 12 (46%) had adverse outcome. Data collection started from median [IQR] of 11.8 [7.0; 24.3] hours (h) of life and lasted until 84.0. [81.8; 87.0] h. During TH, the mean HR of the favorable outcome group was significantly lower than that of the adverse outcome group (86 ± 13/min vs. 104 ± 18/min, p = 0.01). During rewarming HR increased similarly in both groups. SV was unaffected by rewarming, and showed a slowly increasing trend. SV of the favorable outcome group was significantly higher compared to the adverse outcome group (1.55 ± 0.23 ml/kg vs. 1.29 ± 0.30 ml/kg, p = 0.035). In line with this, CO was similar in both groups (136 ± 27 ml/kg/min vs. 134 ± 36 ml/kg/min), and a significant 25% increase in CO was observed during rewarming. Based on multiple regression modeling, HR during TH was independently associated with neurological outcome (p = 0.023).

Conclusion

Based on continuous hemodynamic monitoring, patients with adverse outcome have lower SV and higher HR to achieve similar CO to patients with favorable outcome during TH. HR during hypothermia is independently associated with the neurodevelopmental outcome.
Development and validation of the patient reported outcomes questionnaire of children with asthma in China: A Caregiver's proxy-reported measure
Authors: Xu Tong , Zhang Haiyue , Zhang Yuhai , Yang Peng , Yang Zhe , Jiang Xun , Shang Lei
Published: March 2023

Background

Research on asthma control levels and quality of life is essential for children with asthma during their growth stage. Therefore, it is necessary to develop a questionnaire that can be used for monitoring and evaluating the disease control effectiveness and quality of life of children with asthma in China and to conduct a preliminary evaluation for its reliability, validity, and discriminative ability.

Methods

The questionnaire was created through a literature review and qualitative interviews for a targeted population. Based on the previous work, 30 caregivers of children with asthma and 5 experienced pediatricians reviewed and discussed a collection of items. Then, 72 items were screened and selected to form the draft questionnaire. After three rounds of investigation (with 240, 503, and 360 participants, respectively), the final questionnaire was established according to the evaluation results. The structure of the questionnaire was explored through confirmatory factor analysis. Exploratory factor analysis and variability analysis were applied based on the first two rounds of investigation. Reliability, construct validity, and discriminative ability were evaluated based on the third round of investigation.

Results

The questionnaire contains 6 dimensions and 34 items, and the total cumulative variance contribution rate was 54.96%; Cronbach's α coefficient was 0.91; the split-half reliability coefficient was 0.75, and the test–retest reliability coefficient was 0.74. The children's age, gender, residence, asthma attack in the last three months, caregivers' education background, and monthly income per caregiver were correlated with patient-reported outcomes of children with asthma.

Conclusion

The questionnaire appeared to have good reliability, construct validity, and discriminative ability in children with asthma in China.
Drug exposure during pregnancy: Current understanding and approaches to measure maternal-fetal drug exposure
Authors: Hudson Rachel E. , Metz Torri D. , Ward Robert M. , McKnite Autumn M. , Enioutina Elena Y. , Sherwin Catherine M. , Watt Kevin M. , Job Kathleen M.
Published: March 2023

Prescription drug use is prevalent during pregnancy, yet there is limited knowledge about maternal-fetal safety and efficacy of this drug use because pregnant individuals have historically been excluded from clinical trials. Underrepresentation has resulted in a lack of data available to estimate or predict fetal drug exposure. Approaches to study fetal drug pharmacology are limited and must be evaluated for feasibility and accuracy. Anatomic and physiological changes throughout pregnancy fluctuate based on gestational age and can affect drug pharmacokinetics (PK) for both mother and fetus. Drug concentrations have been studied throughout different stages of gestation and at or following delivery in tissue and fluid biospecimens. Sampling amniotic fluid, umbilical cord blood, placental tissue, meconium, umbilical cord tissue, and neonatal hair present surrogate options to quantify and characterize fetal drug exposure. These sampling methods can be applied to all therapeutics including small molecule drugs, large molecule drugs, conjugated nanoparticles, and chemical exposures. Alternative approaches to determine PK have been explored, including physiologically based PK modeling,in vitromethods, and traditional animal models. These alternative approaches along with convenience sampling of tissue or fluid biospecimens can address challenges in studying maternal-fetal pharmacology. In this narrative review, we 1) present an overview of the current understanding of maternal-fetal drug exposure; 2) discuss biospecimen-guided sampling design and methods for measuring fetal drug concentrations throughout gestation; and 3) propose methods for advancing pharmacology research in the maternal-fetal population.
Mitochondrial CPT1A: Insights into structure, function, and basis for drug development
Authors: Liang Kai
Published: March 2023

Carnitine Palmitoyl-Transferase1A (CPT1A) is the rate-limiting enzyme in the fatty acid β-oxidation, and its deficiency or abnormal regulation can result in diseases like metabolic disorders and various cancers. Therefore, CPT1A is a desirable drug target for clinical therapy. The deep comprehension of human CPT1A is crucial for developing the therapeutic inhibitors like Etomoxir. CPT1A is an appealing druggable target for cancer therapies since it is essential for the survival, proliferation, and drug resistance of cancer cells. It will help to lower the risk of cancer recurrence and metastasis, reduce mortality, and offer prospective therapy options for clinical treatment if the effects of CPT1A on the lipid metabolism of cancer cells are inhibited. Targeted inhibition of CPT1A can be developed as an effective treatment strategy for cancers from a metabolic perspective. However, the pathogenic mechanism and recent progress of CPT1A in diseases have not been systematically summarized. Here we discuss the functions of CPT1A in health and diseases, and prospective therapies targeting CPT1A. This review summarizes the current knowledge of CPT1A, hoping to prompt further understanding of it, and provide foundation for CPT1A-targeting drug development.
Lightweight neural architectures to improve COVID-19 identification
Authors: Hassan Mohammad Mehedi , AlQahtani Salman A. , Alelaiwi Abdulhameed , Papa João P.
Published: March 2023

The COVID-19 pandemic has had a global impact, transforming how we manage infectious diseases and interact socially. Researchers from various fields have worked tirelessly to develop vaccines on an unprecedented scale, while different countries have developed various sanitary protocols to deal with more contagious variants. Machine learning-assisted diagnosis has emerged as a powerful tool that can help health professionals deliver faster and more accurate outcomes. However, medical systems that rely on deep learning often require extensive data, which may be impractical for real-world applications. This paper compares lightweight neural architectures for COVID-19 identification using chest X-rays, highlighting the strengths and weaknesses of each approach. Additionally, a web tool has been developed that accepts chest computer tomography images and outputs the probability of COVID-19 infection along with a heatmap of the regions used by the intelligent system to make this determination. The experiments indicate that most lightweight architectures considered in the study can identify COVID-19 correctly, but further investigation is necessary. Lightweight neural architectures show promise in computer-aided COVID-19 diagnosis using chest X-rays, but they did not reach accuracy rates above 88%, which is necessary for medical applications. These findings suggest that additional research is necessary to improve the accuracy of lightweight models and make them practical for real-world use.
Path analysis method in an epidemic model and stability analysis
Authors: Zhou Yong , Ding Yiming , Guo Minrui
Published: March 2023

In this paper, a new method for obtaining the basic reproduction number is proposed, called the path analysis method. Compared with the traditional next-generation method, this method is more convenient and less error-prone. We develop a general model that includes most of the epidemiological characteristics and enumerate all disease transmission paths. The path analysis method is derived by combining the next-generation method and the disease transmission paths. Three typical examples verify the effectiveness and convenience of the method. It is important to note that the path analysis method is only applicable to epidemic models with bilinear incidence rates. The Volterra-type Lyapunov function is given to prove the global stability of the system. The simulations prove the correctness of our conclusions.
A pilot study on intracerebral hemorrhage imaging based on electrical capacitance tomography
Authors: Xu Rui , Zhuang Wei , Bai Zelin , Wang Feng , Chen Mingsheng , Liu Nan , Jin Gui
Published: March 2023

Introduction: Intracerebral hemorrhage (ICH) is a devastating disease with high rates of mortality and disability. The survival rate and postoperative outcome of ICH can be greatly improved through prompt diagnosis and treatment. CT and MRI are now the gold standards for the diagnosis of ICH, but they are not practical for use in pre-hospital emergencies or at the bedside monitoring.

Methods: Based on the earlier research of ICH detection with a single parallel plate electrode sensor, we developed a 16-electrode Electrical Capacitance Tomography (ECT) system for two-dimensional tomographic imaging of ICH in this study. A 5-layer spherical numerical model and an ex vivo porcine physical model of ICH were created for ECT simulation imaging and actual imaging, respectively, to assess the feasibility of this ECT for ICH imaging.

Results: The bleeding circles were easily seen in the image reconstruction in numerical imaging. In ex vivo imaging, the existence of bleeding was also more clearly shown with the ECT system; however, the position of the bleeding reconstructed in the image was offset by 3 mm from the real site.

Discussion: The study analyzes the causes of this discrepancy and discusses the steps that may be taken to rectify it. Overall, the simulation and ex vivo experimental trials validated the potential of ICH imaging with the ECT method; however, further work is required to increase the performance of the ECT and a more advanced imaging reconstruction algorithm is urgently needed for ICH imaging.
Genome-wide identification and expression profiling reveal the regulatory role of U-box E3 ubiquitin ligase genes in strawberry fruit ripening and abiotic stresses resistance
Authors: Jiang Leiyu , Lin Yuanxiu , Wang Liangxin , Peng Yuting , Yang Min , Jiang Yuyan , Hou Guoyan , Liu Xiaoyang , Li Mengyao , Zhang Yunting , Zhang Yong , Chen Qing , Wang Yan , He Wen , Wang Xiaorong , Tang Haoru , Luo Ya
Published: March 2023

The plant U-box (PUB) proteins are a type of E3 ubiquitin ligases well known for their functions in response to various stresses. They are also related to fruit development and ripening. However, PUB members possess such roles that remain unclear in strawberry. In this study, 155 PUB genes were identified in octoploid strawberry and classified into four groups. Their promoters possessed a variety of cis-acting elements, most of which are associated with abiotic stresses, followed by phytohormones response and development. Protein-protein interaction analysis suggested that FaU-box members could interact with each other as well as other proteins involved in hormone signaling and stress resistance. Transcriptome-based and RT-qPCR expression analysis revealed the potential involvement of FaU-box genes in resistance to stresses and fruit ripening. Of these, FaU-box98 and FaU-box136 were positively while FaU-box52 was negatively related to strawberry ripening. FaU-box98 comprehensively participated in resistance of ABA, cold, and salt, while FaU-box83 and FaU-box136 were broadly associated with drought and salt stresses. FaU-box18 and FaU-box52 were ABA-specific; FaU-box3 was specific to salt stress. In addition, the functional analysis of a randomly selected FaU-box (FaU-box127) showed that the transient overexpression of FaU-box127 promoted the ripening of strawberry fruit, along with significant changes in the expression levels of some ripening-related genes and the content of organic acid and soluble sugar. Overall, these findings provided comprehensive information about the FaU-box gene family and identified the potential FaU-box members participating in stress resistance and strawberry fruit ripening regulation.
Developing and validating a multidimensional Chinese Parental Psychological Control Scale
Authors: Zhu Xiaoqin , Dou Diya , Pan Yangu
Published: March 2023

Introduction

This study validated a Chinese Parental Psychological Control Scale (CPPCS) among secondary school students in mainland China.

Methods

The item pool consisting of 65 items was constructed based on consultation with existing measures and focus group interviews of 19 Chinese adolescents. After content validation conducted by 14 experts, a total of 40 items were retained and subject to further factorial validation based on a sample of 963 Chinese adolescents (mean age = 13.39 ± 0.72; 52.23% females).

Results

Using the two random-split half samples, exploratory and confirmatory factor analyses retained 30 items that loaded on three factors, including “relational induction” (twelve items), “harsh psychological control” (twelve items), and “social comparison shame” (six items). The three-factor structure was invariant across gender (male versus female) and grades (grade 7 versus grade 8) among the whole sample. Cronbach’s αs of the three dimensions in maternal and paternal subscales ranged between 0.89 and 0.92, suggesting adequate internal consistency. The three dimensions were significantly correlated with each other, supporting the scale’s convergent validity. The concurrent validity of the CPPCS was supported by the positive correlations between subscales and parental rejection, and the negative correlations between subscales and parental warmth. In addition, while the “harsh psychological control” and “social comparison shame” negatively predicted adolescents’ well-being, “relational induction” positively predicted adolescents’ well-being.

Discussion

The findings suggest that the CPPCS is a promising instrument for measuring multidimensional psychological control among Chinese parents and for investigating and comparing individual dimensions’ effect on adolescents’ development.
Effectiveness of yoga for major depressive disorder: A systematic review and meta-analysis
Authors: Wu Yufei , Yan Danni , Yang Jianli
Published: March 2023

Objective

Major depressive disorder (MDD) has a relapse rate that cannot be ignored and places a tremendous burden on the patient in the prevention and treatment process. Yoga, a combination of physical and mental exercises, is effective and acceptable for the adjunctive treatment of MDD. This study aimed to explore further the evidence of yoga’s efficacy for patients with MDD.

Methods

PubMed, Embase, Cochrane library, PsycINFO, SinoMed, CNKI, Wanfang, and VIP databases from their inception to 13 October 2022 were searched by a pre-defined search strategy. RCTs of patients with MDD who met diagnostic criteria for yoga treatment were included. RoB2.0 was used to evaluate the quality of the literature. Improvement in depressive symptoms was assessed by the Beck Depression Inventory (BDI), Hamilton Depression Rating Scale (HAMD), or other scales were used as primary outcome indicators, and improvement in anxiety was assessed by the Hamilton Anxiety Scale (HAMA) and State–Trait Anxiety Inventory (STAI) scale as secondary outcome indicators. RR and Cohen’s d at 95% CI were used as effect size estimates, and Q and I2 were used to evaluate the size of heterogeneity, with a p-value less than 0.05 indicating statistical significance.

Results

Thirty-four RCT studies, including 1,269 patients in the treatment group and 1,072 patients in the control group, 48.4% of whom were women, were included in the study. Compared to the control group, the BDI-II results yielded a moderate effect of yoga on the improvement of depressive symptoms (Cohen’s d = −0.60; 95% CI: −1.00 to −0.21; p < 0.01), the HAMD results yielded a moderate improvement of yoga on the severity of depressive symptoms (Cohen’s d = −0.64; 95% CI: −0.98 to −0.30; p < 0.01), and the STAI results can be concluded that yoga had a negligible effect on the improvement of the level of anxiety (Cohen’s d = −0.26; 95% CI: −0.48 to −0.04; p = 0.02). No adverse events occurred in the yoga group during the treatment.

Conclusion

Yoga can improve depressive symptoms and anxiety in patients with MDD and has a safe and wide patient acceptance.

Systematic review registration

[https://www.crd.york.ac.uk/prospero/], identifier [PROSPERO, CRD42022373282].
Social support and quality of life in migrant workers: Focusing on the mediating effect of healthy lifestyle
Authors: Yang Yufan , Zhao Shuzhen , Lin Lulu , Qian Jieyu , Zhang Haiyan , Cai Fuman
Published: March 2023

Objective

To investigate the relationship between social support and quality of life of Chinese migrant workers and to explore the mediating role of healthy lifestyles in social support and quality of life.

Methods

Using a stratified multi-stage sampling method, 1, 298 migrant workers and 983 urban workers across 110 neighborhood committees in five economic development zones in eastern China were surveyed. The social support level of participants was quantified using the Social Support Rating Scale, and quality of life was evaluated using the SF-8. Healthy lifestyle was evaluated based on a combination of sleep, smoking, alcohol consumption, and exercise. Multiple linear regression analysis was used to assess the relationship between quality of life and social support. Stepwise regression was used to analyze the mediating effect of healthy lifestyle, social support, and quality of life among migrant workers.

Results

Total SSRS and total SF-8 scores of migrant workers were significantly higher than those of urban workers (P < 0.001). After controlling for confounders, social support showed an independent positive association with quality of life for both migrant (β = 0.50, P < 0.05) and urban workers (β = 0.62, P < 0.05). Mediation effect analysis revealed that healthy lifestyle partially mediated the relation between social support and quality of life of migrant workers with a mediation effect of 0.07, accounting for 11.70% of the total effect.

Conclusions

This study showed a significant correlation between social support and quality of life of Chinese migrant workers, with healthy lifestyle playing a mediating role. Improving the social support and health literacy of migrant workers and developing a healthy lifestyle are key to improving their quality of life.
A simplified nasopharyngeal swab collection procedure for minimizing patient discomfort while retaining sample quality
Authors: Uršič Tina , Kogoj Rok , Šikonja Jaka , Jevšnik Virant Monika , Petrovec Miroslav
Published: March 2023

A nasopharyngeal swab (NPS) is the most frequently collected sample type when molecular diagnosis of respiratory viruses, including SARS CoV-2, is required. An optimal collection technique would provide sufficient sample quality for the diagnostic process and would minimize the discomfort felt by the patient. This study compares a simplified NPS collection procedure with only one rotation of the swab to a more standard procedure with five rotations. Swabs were collected from 76 healthy volunteers by the same healthcare professional on 2 consecutive days at a similar hour to minimize variability. The number of Ubiquitin C copy number per sample was measured by real-time quantitative PCR and patient discomfort was assessed by questionnaire. No statistically significant difference (p = 0.15) was observed in the Ubiquitin C copy number per sample between a NPS collected with one rotation (5.2 ± 0.6 log UBC number copies/sample) or five rotations (5.3 ± 0.5 log UBC number copies/sample). However, a statistically significant difference was observed in discomfort between these two procedures, the second being much more uncomfortable. Additional analysis of the results showed a weak correlation between discomfort and the number of human cells recovered (Spearman's rho = 0.202) and greater discomfort in younger people. The results of this study show that a NPS collected with one slow rotation has the same quality as a NPS collected with five rotations. However, the collection time is shorter and, most importantly, less unpleasant for patients.