bluuebunny/crossref_metadata_2025 huggingface dataset:
Natural Bioactive Compounds from Fungi as Potential Candidates for Protease Inhibitors and Immunomodulators to Apply for Coronaviruses
Authors: Suwannarach Nakarin , Kumla Jaturong , Sujarit Kanaporn , Pattananandecha Thanawat , Saenjum Chalermpong , Lumyong Saisamorn
Published: April 2020

The inhibition of viral protease is an important target in antiviral drug discovery and development. To date, protease inhibitor drugs, especially HIV-1 protease inhibitors, have been available for human clinical use in the treatment of coronaviruses. However, these drugs can have adverse side effects and they can become ineffective due to eventual drug resistance. Thus, the search for natural bioactive compounds that were obtained from bio-resources that exert inhibitory capabilities against HIV-1 protease activity is of great interest. Fungi are a source of natural bioactive compounds that offer therapeutic potential in the prevention of viral diseases and for the improvement of human immunomodulation. Here, we made a brief review of the current findings on fungi as producers of protease inhibitors and studies on the relevant candidate fungal bioactive compounds that can offer immunomodulatory activities as potential therapeutic agents of coronaviruses in the future.
Relation between Crystal Structures of Precursors and Final Products: Example of Vitamin D Intermediates
Authors: Wanat Monika , Malinska Maura , Kutner Andrzej , Woźniak Krzysztof
Published: April 2020

In this paper, we proved that the solid-state structure of vitamin D analog is well represented by the structures of its structural fragments. This is important in predicting the biological activity of vitamin D analogs that are not available in the solid form. The previously published crystal structure of advanced vitamin D intermediate provided additional insights into vitamin D properties. A similar analysis based on simple vitamin D intermediate analogues showed that precursors crystallized in the space groups typical for vitamins D; geometrical parameters were related to the corresponding parameters in the vitamin D analogues; and crystal structures of the basic intermediates and their final products contained similar intermolecular interactions, essential for the infinite hydrogen bond motif observed in the vitamin D analogues. The energy of these interactions is related as shown by theoretical calculations, that is, energy frameworks analysis. Moreover, analysis of the hydrogen bonds motifs revealed a relation between these motifs and the absolute configuration of basic intermediates as well as the space orientation of the exocyclic methylene group in the final structures.
Natural Compounds Rosmarinic Acid and Carvacrol Counteract Aluminium-Induced Oxidative Stress
Authors: Baranauskaite Juste , Sadauskiene Ilona , Liekis Arunas , Kasauskas Arturas , Lazauskas Robertas , Zlabiene Ugne , Masteikova Ruta , Kopustinskiene Dalia M. , Bernatoniene Jurga
Published: April 2020

Aluminum accumulation, glutathione (GSH) and malondialdehyde (MDA) concentrations as well as catalase (CAT) and superoxide dismutase (SOD) activities were determined in erythrocytes and brain and liver homogenates of BALB/c mice treated with Al3+ (7.5 mg/kg/day (0.15 LD50) as AlCl3 (37.08 mg/kg/day), whereas HCl (30.41 mg/kg/day) was used as Cl− control, the treatments were performed for 21 days, i.p., in the presence and absence of rosmarinic acid (0.2805 mg/kg/day (0.05 LD50), 21 days, i.g.) or carvacrol (0.0405 mg/kg/day (0.05 LD50), 21 days, i.g.). The treatment with AlCl3 increased GSH concentration in erythrocytes only slightly and had no effect on brain and liver homogenates. Rosmarinic acid and carvacrol strongly increased GSH concentration in erythrocytes but decreased it in brain and liver homogenates. However, AlCl3 treatment led to Al accumulation in mice blood, brain, and liver and induced oxidative stress, assessed based on MDA concentration in the brain and liver. Both rosmarinic acid and carvacrol were able to counteract the negative Al effect by decreasing its accumulation and protecting tissues from lipid peroxidation. AlCl3 treatment increased CAT activity in mice brain and liver homogenates, whereas the administration of either rosmarinic acid or carvacrol alone or in combination with AlCl3 had no significant effect on CAT activity. SOD activity remained unchanged after all the treatments in our study. We propose that natural herbal phenolic compounds rosmarinic acid and carvacrol could be used to protect brain and liver against aluminum induced oxidative stress leading to lipid peroxidation.
Residues of Persistent Organic Pollutants (POPs) in Agricultural Soils Adjacent to Historical Sources of Their Storage and Distribution—The Case Study of Azerbaijan
Authors: Ukalska-Jaruga Aleksandra , Lewińska Karolina , Mammadov Elton , Karczewska Anna , Smreczak Bożena , Medyńska-Juraszek Agnieszka
Published: April 2020

The aim of this study was to identify and examine the levels of organochlorine pesticides (OCPs), polycyclic aromatic hydrocarbons (PAHs), and polychlorinated biphenyls (PCBs) in soil collected from the surroundings of historical pesticide storage facilities on former agricultural aerodromes, warehouses, and pesticide distribution sites located in the most important agricultural regions in Azerbaijan. The conducted research included determination of three groups of POPs (occurring together), in the natural soil environment influenced for many years by abiotic and biotic factors that could have caused their transformations or decomposition. In this study, soil samples were collected in 21 georeferenced points located in the administrative area of Bilasuvar, Saatly, Sabirabad, Salyan and Jalilabad districts of Azerbaijan. Soil chemical analysis involved determination of organochlorine compounds (OCP): hexachlorocyclohexanes (HCHs) (three isomers α-HCH, β-HCH and γ-HCH) and dichlorodiphenyltrichloroethanes (DDTs) (six congeners 2,4′DDT; 4,4′DDT; 2,4′DDE; 4,4′DDE; 2,4′DDE; and 4,4′DDE); polycyclic aromatic hydrocarbons (PAHs): 16 compounds from the United States Environmental Protection Agency US EPA list and, PCBs (seven congeners identified with the following IUPAC numbers: 28, 52, 101, 118, 138, 153, and 180). Our research showed that OCPs reached the highest concentration in the studied areas. The total concentrations of OCPs ranged from 0.01 to 21,888 mg∙kg−1 with significantly higher concentrations of Σ6DDTs (0.01 μg kg−1 to 21880 mg kg−1) compared to ΣHCH (0.14 ng kg−1 to 166.72 µg kg−1). The total concentrations of PCBs in the studied soils was varied from 0.02 to 147.30 μg·kg−1 but only PCB138 and PCB180 were detected in all analyzed samples. The concentrations of Σ16 PAHs were also strongly diversified throughout the sampling areas and ranged from 0.15 to 16,026 mg kg−1. The obtained results confirmed that the agricultural soils of Azerbaijan contained much lower (up to by three orders of magnitude) concentrations of PCBs and PAHs than DDT. It is supported by the fact that PCBs and PAHs were not directly used by agriculture sector and their content results from secondary sources, such as combustion and various industrial processes. Moreover, the high concentrations of PAHs in studied soils were associated with their location in direct neighborhood of the airport, as well as with accumulation of contaminants from dispersed sources and long range transport. The high concentrations of pesticides confirm that deposition of parent OCPs have occurred from obsolete pesticide landfills.
Electrospun 3D Structured Carbon Current Collector for Li/S Batteries
Authors: Kalybekkyzy Sandugash , Mentbayeva Almagul , Yerkinbekova Yerkezhan , Baikalov Nurzhan , Kahraman Memet Vezir , Bakenov Zhumabay
Published: April 2020

Light weight carbon nanofibers (CNF) fabricated by a simple electrospinning method and used as a 3D structured current collector for a sulfur cathode. Along with a light weight, this 3D current collector allowed us to accommodate a higher amount of sulfur composite, which led to a remarkable increase of the electrode capacity from 200 to 500 mAh per 1 g of the electrode including the mass of the current collector. Varying the electrospinning solution concentration enabled obtaining carbonized nanofibers of uniform structure and controllable diameter from several hundred nanometers to several micrometers. The electrochemical performance of the cathode deposited on carbonized PAN nanofibers at 800 °C was investigated. An initial specific capacity of 1620 mAh g−1 was achieved with a carbonized PAN nanofiber (cPAN) current collector. It exhibited stable cycling over 100 cycles maintaining a reversible capacity of 1104 mAh g−1 at the 100th cycle, while the same composite on the Al foil delivered only 872 mAh g−1. At the same time, 3D structured CNFs with a highly developed surface have a very low areal density of 0.85 mg cm−2 (thickness of ~25 µm), which is lower for almost ten times than the commercial Al current collector with the same thickness (7.33 mg cm−2).
Laser-Assisted Fabrication for Metal Halide Perovskite-2D Nanoconjugates: Control on the Nanocrystal Density and Morphology
Authors: Kostopoulou Athanasia , Brintakis Konstantinos , Serpetzoglou Efthymis , Stratakis Emmanuel
Published: April 2020

We report on a facile and rapid photo-induced process to conjugate graphene-based materials with metal-halide perovskite nanocrystals. We show that a small number of laser pulses is sufficient to decorate the 2-dimensional (2D) flakes with metal-halide nanocrystals without affecting their primary morphology. At the same time, the density of anchored nanocrystals could be finely tuned by the number of irradiation pulses. This facile and rapid room temperature method provides unique opportunities for the design and development of perovskite-2D nanoconjugates, exhibiting synergetic functionality by combining nanocrystals of different morphologies and chemical phases with various 2D materials.
Fe-Cu Doped Multiwalled Carbon Nanotubes for Fenton-like Degradation of Paracetamol Under Mild Conditions
Authors: Barrios-Bermúdez Niurka , González-Avendaño Marta , Lado-Touriño Isabel , Cerpa-Naranjo Arisbel , Rojas-Cervantes María
Published: April 2020

A series of carbon nanotubes doped with Fe and/or Cu, Fe100−xCux/CNT (x = 0, 25, 50, 75 and 100) has been prepared by an easy method of wetness impregnation of commercial multiwalled carbon nanotubes previously oxidized with nitric acid. The wide characterization of the solids by different techniques demonstrates that the incorporation of Fe and Cu to the CNTs has been successfully produced. Fe100−xCux/CNT samples were tested as catalysts in the removal of paracetamol from aqueous solution by a combined process of adsorption and Fenton-like oxidation. Under mild conditions, 25 °C and natural pH of solution, i.e., nearly neutral, values of oxidation of paracetamol between 90.2% and 98.3% were achieved after 5 h of reaction in most of cases. Furthermore, with the samples containing higher amounts of copper, i.e., Cu100/CNT and Fe25Cu75/CNT, only 2 h were necessary to produce depletion values of 73.2% and 87.8%, respectively. The influence of pH and dosage of H2O2 on the performance has also been studied. A synergic effect between both Cu+/Cu2+ and Fe2+/Fe3+ in Fenton-like reaction was observed. These results demonstrate that Fe100−xCux/CNT are powerful Fenton-like catalyst for degradation of paracetamol from aqueous solution and they could be extended to the removal of other organic pollutants.
Preparation and Evaluation of Nanofibrous Hydroxypropyl Cellulose and β-Cyclodextrin Polyurethane Composite Mats
Authors: Gradinaru Luiza Madalina , Barbalata-Mandru Mihaela , Drobota Mioara , Aflori Magdalena , Spiridon Maria , Gradisteanu Pircalabioru Gratiela , Bleotu Coralia , Butnaru Maria , Vlad Stelian
Published: April 2020

A series of nanofibrous composite mats based on polyurethane urea siloxane (PUUS), hydroxypropyl cellulose (HPC) and β-cyclodextrin (β-CD) was prepared using electrospinning technique. PUUS was synthesized by two steps solution polymerization procedure from polytetramethylene ether glycol (PTMEG), dimethylol propionic acid (DMPA), 4,4′-diphenylmethane diisocyanate (MDI) and 1,3-bis-(3-aminopropyl) tetramethyldisiloxane (BATD) as chain extender. Then, the composites were prepared by blending PUUS with HPC or βCD in a ratio of 9:1 (w/w), in 15% dimethylformamide (DMF). The PUUS and PUUS based composite solutions were used for preparation of nanofibrous mats. In order to identify the potential applications, different techniques were used to evaluate the chemical structure (Fourier transform infrared-attenuated total reflectance spectroscopy—FTIR-ATR), morphological structure (Scanning electron microscopy—SEM and Atomic force microscopy—AFM), surface properties (contact angle, dynamic vapors sorption—DVS), mechanical characteristics (tensile tests), thermal (differential scanning calorimetry—DSC) and some preliminary tests for biocompatibility and microbial adhesion.
Occurrence of Blastocystis in Patients with Clostridioides difficile Infection
Authors: Vega Laura , Herrera Giovanny , Muñoz Marina , Patarroyo Manuel Alfonso , Ramírez Juan David
Published: April 2020

Clostridiodes difficile comprises a public-health threat that has been understudied in Colombia. Hypervirulent strains of C. difficile harbor multiple toxins, can be easily spread, and can have their onset of disease within healthcare facilities (HCFO) and the community (CO). Studies have shown that a disrupted microbiota (e.g., dysbiosis) may allow C. difficile infection (CDI). It has been suggested that dysbiosis prevents colonization by the anaerobic eukaryote Blastocystis, possibly due to an increase in luminal oxygen tension. No study has found co-occurrence of CDI and Blastocystis. Therefore, we aimed to determine the frequencies of C. difficile and Blastocystis infection/colonization in 220 diarrheal fecal samples. Molecular detection by PCR for both microorganisms was performed, with descriptive analyses of four variables (CDI detection, determination of C. difficile toxigenic profiles, Blastocystis detection, and patient site of onset). We demonstrate a significant association between the presence of Blastocystis and CDI, with coinfection found in 61 patients, and show a high frequency of CDI among both HCFO and CO groups. Our results of coinfection frequencies could support hypotheses that suggest Blastocystis can adapt to dysbiosis and oxidative stress. Further, the presence of toxigenic C. difficile occurring outside healthcare facilities shown here raises the alarm for community wide spread.
Characterization of an MLP Homologue from Haemaphysalis longicornis (Acari: Ixodidae) Ticks
Authors: Luo Jin , Shen Hui , Ren Qiaoyun , Guan Guiquan , Zhao Bo , Yin Hong , Chen Ronggui , Zhao Hongying , Luo Jianxun , Li Xiangrui , Liu Guangyuan
Published: April 2020

Members of the cysteine-rich protein (CRP) family are known to participate in muscle development in vertebrates. Muscle LIM protein (MLP) belongs to the CRP family and has an important function in the differentiation and proliferation of muscle cells. In this study, the full-length cDNA encoding MLP from Haemaphysalis longicornis (H. longicornis; HLMLP) ticks was obtained by 5′ rapid amplification of cDNA ends (RACE). To verify the transcriptional status of MLP in ticks, HLMLP gene expression was assessed during various developmental stages by real-time PCR (RT-PCR). Interestingly, HLMLP expression in the integument was significantly (P < 0.01) higher than that observed in other tested tissues of engorged adult ticks. In addition, HLMLP mRNA levels were significantly downregulated in response to thermal stress at 4 °C for 48 h. Furthermore, recombinant HLMLP was expressed in Escherichia coli, and Western blot analysis showed that rabbit antiserum against H. longicornis adults recognized HLMLP and MLPs from different ticks. Ten 3-month-old rabbits that had never been exposed to ticks were used for the immunization and challenge experiments. The rabbits were divided into two groups of five rabbits each, where rabbits in the first group were immunized with HLMLP, while those in the second group were immunized with phosphate-buffered saline (PBS) diluent as controls. The vaccination of rabbits with the recombinant HLMLP conferred partial protective immunity against ticks, resulting in 20.00% mortality and a 17.44% reduction in the engorgement weight of adult ticks. These results suggest that HLMLP is not ideal as a candidate for use in anti-tick vaccines. However, the results of this study generated novel information on the MLP gene in H. longicornis and provide a basis for further investigation of the function of this gene that could potentially lead to a better understanding of the mechanism of myofiber determination and transformation
Foetal Immune Response Activation and High Replication Rate during Generation of Classical Swine Fever Congenital Infection
Authors: Bohórquez José Alejandro , Muñoz-González Sara , Pérez-Simó Marta , Muñoz Iván , Rosell Rosa , Coronado Liani , Domingo Mariano , Ganges Llilianne
Published: April 2020

Classical swine fever virus (CSFV) induces trans-placental transmission and congenital viral persistence; however, the available information is not updated. Three groups of sows were infected at mid-gestation with either a high, moderate or low virulence CSFV strains. Foetuses from sows infected with high or low virulence strain were obtained before delivery and piglets from sows infected with the moderate virulence strain were studied for 32 days after birth. The low virulence strain generated lower CSFV RNA load and the lowest proportion of trans-placental transmission. Severe lesions and mummifications were observed in foetuses infected with the high virulence strain. Sows infected with the moderately virulence strain showed stillbirths and mummifications, one of them delivered live piglets, all CSFV persistently infected. Efficient trans-placental transmission was detected in sows infected with the high and moderate virulence strain. The trans-placental transmission occurred before the onset of antibody response, which started at 14 days after infection in these sows and was influenced by replication efficacy of the infecting strain. Fast and solid immunity after sow vaccination is required for prevention of congenital viral persistence. An increase in the CD8+ T-cell subset and IFN-alpha response was found in viremic foetuses, or in those that showed higher viral replication in tissue, showing the CSFV recognition capacity by the foetal immune system after trans-placental infection.
NH4+ Toxicity, Which Is Mainly Determined by the High NH4+/K+ Ratio, Is Alleviated by CIPK23 in Arabidopsis
Authors: Shi Sujuan , Xu Fangzheng , Ge Yuqian , Mao Jingjing , An Lulu , Deng Shuaijun , Ullah Zia , Yuan Xuefeng , Liu Guanshan , Liu Haobao , Wang Qian
Published: April 2020

Ammonium (NH4+) toxicity is always accompanied by ion imbalances, and NH4+ and potassium (K+) exhibit a competitive correlation in their uptake and transport processes. In Arabidopsis thaliana, the typical leaf chlorosis phenotype in the knockout mutant of calcineurin B-like interacting protein kinase 23 (CIPK23) is high-NH4+-dependent under low-K+ condition. However, the correlation of K+ and NH4+ in the occurrence of leaf chlorosis in the cipk23 mutant has not been deeply elucidated. Here, a modified hydroponic experimental system with different gradients of NH4+ and K+ was applied. Comparative treatments showed that NH4+ toxicity, which is triggered mainly by the high ratio of NH4+ to K+ (NH4+/K+ ≥ 10:1 for cipk23) but not by the absolute concentrations of the ions, results in leaf chlorosis. Under high NH4+/K+ ratios, CIPK23 is upregulated abundantly in leaves and roots, which efficiently reduces the leaf chlorosis by regulating the contents of NH4+ and K+ in plant shoots, while promoting the elongation of primary and lateral roots. Physiological data were obtained to further confirm the role CIPK23 in alleviating NH4+ toxicity. Taken all together, CIPK23 might function in different tissues to reduce stress-induced NH4+ toxicity associated with high NH4+/K+ ratios by regulating the NH4+–K+ balance in Arabidopsis.
A Chemometric Tool to Monitor and Predict Cell Viability in Filamentous Fungi Bioprocesses Using UV Chromatogram Fingerprints
Authors: Doppler Philipp , Veiter Lukas , Spadiut Oliver , Herwig Christoph , Rajamanickam Vignesh
Published: April 2020

Monitoring process variables in bioprocesses with complex expression systems, such as filamentous fungi, requires a vast number of offline methods or sophisticated inline sensors. In this respect, cell viability is a crucial process variable determining the overall process performance. Thus, fast and precise tools for identification of key process deviations or transitions are needed. However, such reliable monitoring tools are still scarce to date or require sophisticated equipment. In this study, we used the commonly available size exclusion chromatography (SEC) HPLC technique to capture impurity release information in Penicillium chrysogenum bioprocesses. We exploited the impurity release information contained in UV chromatograms as fingerprints for development of principal component analysis (PCA) models to descriptively analyze the process trends. Prediction models using well established approaches, such as partial least squares (PLS), orthogonal PLS (OPLS) and principal component regression (PCR), were made to predict the viability with model accuracies of 90% or higher. Furthermore, we demonstrated the platform applicability of our method by monitoring viability in a Trichoderma reesei process for cellulase production. We are convinced that this method will not only facilitate monitoring viability of complex bioprocesses but could also be used for enhanced process control with hybrid models in the future.
Hydrodynamic and Heat Transfer Study of a Fluidized Bed by Discrete Particle Simulations
Authors: Mu Lijing , Buist Kay A. , Kuipers J. A. M. , Deen Niels G.
Published: April 2020

A numerical simulation study was carried out to study the combined thermal behavior and hydrodynamics of a pseudo-2D fluidized bed using a computational fluid dynamics–discrete element method (CFD-DEM). To mimic the effect of heterogeneous exothermic reactions, a constant heat source was implemented in the particle energy equation. The effects of superficial gas velocity, bed height and heat source distribution were analyzed with the aid of averaged volume fraction and temperature distributions and velocity profiles. It was found that both the gas superficial velocity and the bed aspect ratio have a profound influence on fluidization behavior and temperature distributions.
Analysis of Reference Ranges of Total Serum Protein in Namibia: Clinical Implications
Authors: Henok Josephine N. , Okeleye Benjamin I. , Omodanisi Elizabeth I. , Ntwampe Seteno K. O. , Aboua Yapo G.
Published: April 2020

A reference range is an essential part of clinical laboratory test interpretation and patient care. The levels of total serum protein (TSP) are measured in sera to assess nutritional, liver, and kidney disorders. This study determined the TSP reference range with respect to gender, age, and region in Namibia. A retrospective cross-sectional study was conducted to determine the TSP reference range among 78,477 healthy participants within the ages of less than one year to more than 65 yrs in 14 regions of Namibia. The reference range of TSP was 51–91 g/L for females and 51–92 g/L for males. A reduced TSP range of 48.00–85.55 g/L (2.5–97.5 percentiles) was established at <1–5 years and increased towards adolescence. An uttermost range of 54–93 g/L was observed from 36–65 years of age. At the age >65 years; a steady decline in the reference range (51.00–89 g/L) was recorded. An upper TSP range of 53–92 g/L (2.5–97.5 percentiles) was detected in Erongo, Zambezi, Hardap, Kavango East, and a comparable trend was also seen in Omusati with a 54–91 g/L range. Meanwhile; a reduced TSP range of 50–89 g/L was identified in Ohangwena. This study showed that gender, age, and geographical location can impact TSP levels with a significant clinical difference (p < 0.05) between each category.
Dabo Swinney, Universal Whiteness, and a “Sin Problem”
Authors: Scholes Jeffrey
Published: April 2020

Clemson University head football coach Dabo Swinney was asked to respond to Colin Kaepernick’s protest of police killings of unarmed black men and women by kneeling for the national anthem. Swinney’s response was surprisingly comprehensive and illuminating into his stance on race, religion, and sport. He crystallizes his overall interpretation of societal problems with the statement, “It’s so easy to say we have a race problem, but we got a sin problem.” In this essay, I examine “whiteness” as that which endows whites with a kind of universal authority to establish norms as well as provide a protective cloak of invisibility that effectively hides the identity of those constructing the norms. I argue that Swinney’s unconscious display of his own whiteness coupled with the additional cloak of universal sin, that purportedly knows no color, serves to downplay and dismiss Kaepernick’s call for racial justice.
Reimagining Christian Hope(lessness) in the Anthropocene
Authors: Robinson Timothy
Published: April 2020

Faith in the Anthropocene requires a re-imagined account of Christian hope. Research on the emergence of eco-anxiety disorder shows that climate crisis and ecological destruction have psychological and emotional effects on persons and communities, producing fear, despair, and hopelessness. Accounts of hope in recent environmental literature and in traditional Christian formulations rely on faith in political will, technological innovation, or an omnipotent divine sovereign to intervene and save. Such accounts are inadequate for this moment. A re-imagined notion of Christian hope will embrace hopelessness, understood as the relinquishment of false optimism that the climate crisis can be reversed and a commitment to act without expectation of success, but with a commitment to nurturing the wisdom to live more humanly.
Sustainability in Maritime Sector: Waste Management Alternatives Evaluated in a Circular Carbon Economy Perspective
Authors: Gallo Michela , Moreschi Luca , Mazzoccoli Michela , Marotta Veronica , Del Borghi Adriana
Published: April 2020

Sustainability and waste management on board are key issues that need to be addressed by the maritime sector also in terms of greenhouse gas emissions (GHG). With the aim of evaluating waste management alternatives in a circular economy perspective, the study examines a combined system for the optimisation of ship waste management and assesses its possible use for energy purposes. Different systems are analysed in relation to their GHG emission reduction potential regardless of routes and ports of destination. A SWOT (Strengths, Weaknesses, Opportunities and Threats) analysis was carried out on waste management alternatives in order to preliminary identify their potential in terms of GHG emissions reduction, cost, environmental sustainability, methodological coherence, feasibility and replicability. Following this analysis, two case studies of particular interest were identified: (1) the thermo-chemical treatment of waste oils and sludge to obtain fuel oils; (2) the installation of a waste-to-energy plant and subsequent energy recovery on board. UNFCCC (United Nations Framework Convention on Climate Change) methodologies were applied to these two case studies to calculate GHG emission reduction resulting from their implementation. The obtained results are presented with the aim of supporting sustainable waste management strategies on board in a circular carbon economy perspective.
In Vivo Dielectric Properties of Healthy and Benign Rat Mammary Tissues from 500 MHz to 18 GHz
Authors: Yilmaz Tuba , Ates Alkan Fatma
Published: April 2020

This work investigates the in vivo dielectric properties of healthy and benign rat mammary tissues in an attempt to expand the dielectric property knowledge of animal models. The outcomes of this study can enable testing of microwave medical technologies on animal models and interpretation of tissue alteration-dependent in vivo dielectric properties of mammary tissues. Towards this end, in vivo dielectric properties of healthy rat mammary tissues and chemically induced benign rat mammary tumors including low-grade adenosis, sclerosing adenosis, and adenosis were collected with open-ended coaxial probes from 500 MHz to 18 GHz. The in vivo measurements revealed that the dielectric properties of benign rat mammary tumors are higher than the healthy rat mammary tissues by 9.3% to 35.5% and 19.6% to 48.7% for relative permittivity and conductivity, respectively. Furthermore, to our surprise, we found that the grade of the benign tissue affects the dielectric properties for this study. Finally, a comparison with ex vivo healthy human mammary tissue dielectric properties revealed that the healthy rat mammary tissues best replicate the dielectric properties of healthy medium density human samples.
Accessible Real-Time Surveillance Radar System for Object Detection
Authors: Park Seongha , Kim Yongho , Lee Kyuhwan , Smith Anthony H. , Dietz James E. , Matson Eric T.
Published: April 2020

As unmanned ground and aerial vehicles become more accessible and their usage covers a wider area of application, including for threatening purposes which can cause connected catastrophe, a surveillance system for the public places is being considered more essential to respond to those possible threats. We propose an inexpensive, lighter, safer, and smaller radar system than military-grade radar systems while keeping reasonable capability for use in monitoring public places. The paper details the iterative process on the system design and improvements with experiments to realize the system used for surveillance. The experiments show the practical use of the system and configuration for a better understanding of using the system. Cyber-physical systems for outdoor environments can benefit from the system as a sensor for sensing objects as well as monitoring.
A Deep Learning-Based Method for Overhead Contact System Component Recognition Using Mobile 2D LiDAR
Authors: Chen Lipei , Xu Cheng , Lin Shuai , Li Siqi , Tu Xiaohan
Published: April 2020

The overhead contact system (OCS) is a critical railway infrastructure for train power supply. Periodic inspections, aiming at acquiring the operational condition of the OCS and detecting problems, are necessary to guarantee the safety of railway operations. One of the OCS inspection means is to analyze data of point clouds collected by mobile 2D LiDAR. Recognizing OCS components from the collected point clouds is a critical task of the data analysis. However, the complex composition of OCS makes the task difficult. To solve the problem of recognizing multiple OCS components, we propose a new deep learning-based method to conduct semantic segmentation on the point cloud collected by mobile 2D LiDAR. Both online data processing and batch data processing are supported because our method is designed to classify points into meaningful categories of objects scan line by scan line. Local features are important for the success of point cloud semantic segmentation. Thus, we design an iterative point partitioning algorithm and a module named as Spatial Fusion Network, which are two critical components of our method for multi-scale local feature extraction. We evaluate our method on point clouds where sixteen categories of common OCS components have been manually labeled. Experimental results show that our method is effective in multiple object recognition since mean Intersection-over-Unions (mIoUs) of online data processing and batch data processing are, respectively, 96.12% and 97.17%.
An Indoor DFEC Ranging Method for Homologous Base Station Based on GPS L1 and BeiDou B1 Signals
Authors: Zhang Heng , Pan Shuguo , Sheng Chuanzhen , Gan Xingli , Yu Baoguo , Huang Lu , Li Yaning
Published: April 2020

High-precision navigation and positioning technology for indoor areas has become one of the research hotspots in the current navigation field. However, due to the complexity of the indoor environment, this technology direction is also one of the research difficulties. At present, our common indoor positioning methods are WIFI, Bluetooth, LED, ultrasound and pseudo satellite. However, due to the problem of inaccurate direct or indirect ranging, the positioning accuracy is usually affected, which makes the final application difficult to achieve. In order to avoid the ranging limitations of the existing methods, a new dual-frequency entanglement constraint (DFEC) ranging method based on homologous base station is proposed in this paper. The relationship between the homologous characteristics of dual-frequency signals and the phase relationship within the cycle is used to estimate the current carrier phase adjustment the true value of the cycle count is used to get rid of the constraints of the ranging conditions and improve the ranging accuracy. In order to verify the feasibility of this method, the wired environment test and the typical characteristic points of wireless environment are tested and analyzed respectively. The analysis results show that in the wired environment, the transmitting base station and the receiving terminal will introduce a ranging error of one wavelength; in the wireless environment, due to the influence of spatial noise and multipath, the error of the estimation of the whole cycles of the ranging value increases significantly. And this phenomenon is most obvious especially in the region where the signal is shaded, but the error estimate that satisfies ± 1 wavelength still accounts for 90%. Based on this, we conduct multiple observation data collection at five typical feature points, and used existing MATLAB positioning algorithms to conduct positioning error tests. The analysis found that under this error condition, the positioning accuracy was about 0.6 m, and 93% of the points met the 1-m positioning accuracy.
Human–Nature Relationships in East Asian Animated Films
Authors: Pan Yuan
Published: April 2020

Our relationship with nature is complex and exploring this extends beyond academia. Animated films with powerful narratives can connect humans with nature in ways that science cannot. Narratives can be transformative and shape our opinions. Nevertheless, there is little research into non-Western films with strong conservation themes. Hayao Miyazaki is a Japanese filmmaker that is acknowledged as one of the greatest animated filmmakers and master storytellers globally. The themes of environmentalism, feminism and pacifism resonate throughout his films. His underlying message is that humans must strive to live in harmony with nature, whilst presenting us with the socio-cultural complexities of human–nature relationships. I review five of Miyazaki’s films that explore human–nature relationships. One film was released with a special recommendation from the World Wildlife Fund for Nature (WWF) and the other won an Oscar. I explore the lessons that we can learn from these films regarding human–nature relationships, and how to create powerful narratives that resonate with audiences and transcend cultural barriers.
Big Data in Education: Perception of Training Advisors on Its Use in the Educational System
Authors: Ruiz-Palmero Julio , Colomo-Magaña Ernesto , Ríos-Ariza José Manuel , Gómez-García Melchor
Published: April 2020

Big Data has revolutionized decision making in many fields, including education. The incorporation of information and communication technologies into education enables us to gather information about the teaching and learning process. As Big Data can help us improve it, it is paramount to integrate it into initial and continuous learning stages. This study therefore aims at finding out the perception of the training advisors of teacher training centers (N = 117) in Andalusia on the application of Big Data in education. The tool is an adaptation of the VABIDAE (Assessment of Big Data Applied to Education) scale, and the study of the descriptive statistics was carried out by using the analysis of variance (ANOVA) and Mann–Whitney U tests in order to check the existence of significant differences and correlations between the items that make up the scale. The results reflect the positive perception of training advisors on the use of Big Data in education. Significant differences were found in the competence level variable, whereby this tool was better rated by those advisors who feel that they have an advanced competence level. In conclusion, Big Data is valued for its ability to personalize educational processes and the consequent improvement in academic results, which shows the need to increase the level of knowledge about this tool.
Sustainable Water Use Considering Three Hungarian Dairy Farms
Authors: Nagypál Virág , Mikó Edit , Hodúr Cecilia
Published: April 2020

Sustainable water management is one of the biggest challenges in the 21st century as availability of fresh water resources is under depletion. Growing population, extreme weather conditions (drought, fire, flood), and increasing global food demand all result in higher water consumption by humans. Assessing qualitative and quantitative deterioration of fresh water supplies is crucial in water scarcity areas. By identifying blue, green and grey water components, water use can be assessed in a more comprehensive way. Water use assessment on a dairy farm is influenced by several factors such as chosen breed, herd size, keeping, feeding and milking technology. Productivity level of milking cows, amount of daily milking and type of litter (straw or liquid manure) have impact on water use by technology and cattle. If these factors are assessed and their proportion within the total water use is identified or calculated, dairy farmers are able to analyze water management precisely and shift to more sustainable solutions. The aim of this research is to analyze and to compare the impact of different keeping systems, i.e., traditional and modern, and milking technologies, i.e., robotic milking system, parallel and polygon parlors, on the water use of dairy farms to give a guide to dairy experts and to find opportunities where water recycling/reuse might be applicable.
Analyzing Profitability and Discount Rates for Solar PV Plants. A Spanish Case
Authors: Guaita-Pradas Inmaculada , Blasco-Ruiz Ana
Published: April 2020

The widespread use of renewable energy sources and the growing concern about climate change, together with Spain’s exceptional weather and solar radiation conditions, have led to an increase in the use of photovoltaics for energy production in the country. Solar power generation has been tightly regulated, although the legal framework has changed frequently over the years. When assessing the potential financial performance of any business venture, legal as well as financial aspects must be considered, but a critical factor is the discount rate used, which must reflect the company’s capital cost. Other factors are the period of interest, the firm’s activity, market risk, and the level of debt of firms in the sector. The main objective of this study is thus to estimate the discount rate for companies using photovoltaics to produce solar power. We calculate it by employing two financial techniques: capital asset pricing model and historical return analysis. We then evaluate the investment in a photovoltaic plant with a capacity of 5000 kW located in eastern Spain, assuming it started its activity in different years which coincide with changes in the regulatory framework. The results show the relevance of the initial outlay costs for the profitability of photovoltaic power plants.
Rickettsial Diseases: Not Uncommon Causes of Acute Febrile Illness in India
Authors: Biswal Manisha , Krishnamoorthi Sivanantham , Bisht Kamlesh , Sehgal Amit , Kaur Jasleen , Sharma Navneet , Suri Vikas , Sethi Sunil
Published: April 2020

Rickettsial diseases (RDs) are major under-diagnosed causes of arthropod borne acute febrile illness (AFI) presenting with a range of symptoms from mild self-limiting fever to fatal sepsis. The spotted fever group (SFG) and typhus group (TG) are major RDs, which are commonly caused by Rickettsia conorii and Rickettsia typhi, respectively. The limited availability and role of serological tests in the acute phase of illness warrants rapid reliable molecular methods for diagnosis and epidemiological studies. Two hundred patients with AFI in whom the routine fever diagnostics were negative, were enrolled over a period of two months (April 2019 to May 2019). DNA was extracted and in-house nested PCR using primers specific for both SPG and TG pathogens was used. The positive amplified products were sequenced for species identification and phylogenetic analysis was performed using MEGA 7.0.14 software (iGEM, Temple University, Philadelphia, PA 19122, USA). The demographic details of the RD cases were documented. The prevalence of RD among AFI cases was 7% (14/200); SFG and TG were identified as the cause in 4% and 3% of AFI cases, respectively. The median age of the RD cases was 22 years (range 2–65). The median duration of fever was 3 days (range 1–12). The RD cases presented with respiratory symptoms or signs (44.44%), jaundice (22.22%), abdominal pain (22.22%), diarrhea (22.22), vesicular rash (11.11%), vomiting (11.11%), loss of appetite (11.11%), headache (11.11%), leukocytosis (88.88% with mean count 22,750/mm3), and thrombocytopenia (33.33%). The cases were treated empirically with piperacillin-tazobactam (66.66%), clindamycin (44.44%), cefotaxime (33.33%), meropenem (33.33%), metronidazole (33.33%), doxycycline (22.22%), azithromycin (22.22%), ceftriaxone (11.11%), and amoxicillin-clavulanic acid (11.11%). The mortality among the RD cases was 11.11%. The present pilot study shows that RD is not an uncommon cause of AFI in north India. The febrile episodes are usually transient, not severe and associated with heterogenous clinical presentation without documented history of tick exposure in the hospitalized patients. The transient, non-severe, febrile illness could be due to transient rickettsemia resulting from empirical antimicrobial therapy as the rickettsial organisms are expected to be more susceptible to higher doses of β-lactam antibiotics. The study emphasizes the molecular method as a useful tool to identify rickettsial etiology in AFI.
Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant
Authors: Cui Xuemei , Wang Yong , Guan Ran , Lu Meiqian , Yuan Lijia , Xu Wei , Hu Songhua
Published: April 2020

Our previous study demonstrated that a vegetable oil consisting of soybean oil, vitamin E, and ginseng saponins (SO-VE-GS) had an adjuvant effect on a foot-and-mouth disease (FMD) vaccine in a mouse model. The present study was to compare the adjuvant effects of SO-VE-GS and the conventional ISA 206 on an FMD vaccine in Hu sheep. Animals were intramuscularly (i.m.) immunized twice at a 3-week interval with 1 mL of an FMD vaccine adjuvanted with SO-VE-GS (n = 10) or ISA 206 (n = 9). Animals without immunization served as control (n = 10). Blood was sampled prior to vaccination and at 2, 4, 6, and 8 weeks post the booster immunization to detect FMD virus (FMDV)-specific IgG. Blood collected at 8 weeks after the booster was used for the analyses of IgG1 and IgG2, serum neutralizing (SN) antibody, IL-4 and IFN-γ production, and proteomic profiles. The results showed that IgG titers rose above the protection level (1:128) in SO-VE-GS and ISA 206 groups after 2 and 4 weeks post the booster immunization. At 6 weeks post the booster, the ISA 206 group had 1 animal with IgG titer less than 1:128 while all the animals in the SO-VE-GS group retained IgG titers of more than 1:128. At 8 weeks post the booster, 6 of 9 animals had IgG titers less than 1:128 with a protective rate of 33.3% in the ISA 206 group, while only 1 of 10 animals had IgG titer less than 1:128 with a protective rate of 90% in the SO-VE-GS group, with statistical significance. In addition, IgG1, IgG2, SN antibodies, IL-4, and IFN-γ in the SO-VE-GS group were significantly higher than those of the ISA 206 group. Different adjuvant effects of SO-VE-GS and ISA 206 may be explained by the different proteomic profiles in the two groups. There were 39 and 47 differentially expressed proteins (DEPs) identified in SO-VE-GS compared to the control or ISA 206 groups, respectively. In SO-VE-GS vs. control, 3 immune related gene ontology (GO) terms and 8 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were detected, while 2 immune related GO terms and 5 KEGG pathways were found in ISA 206 vs. control. GO and KEGG analyses indicated that ‘positive regulation of cytokine secretion’, ‘Th1/Th2 cell differentiation’, and ‘Toll-like receptor signaling pathways’, were obviously enriched in the SO-VE-GS group compared to the other groups. Coupled with protein–protein interaction (PPI) analysis, we found that B7TJ15 (MAPK14) was a key DEP for SO-VE-GS to activate the immune responses in Hu sheep. Therefore, SO-VE-GS might be a promising adjuvant for an FMD vaccine in Hu sheep.
Tumor Membrane Vesicle Vaccine Augments the Efficacy of Anti-PD1 Antibody in Immune Checkpoint Inhibitor-Resistant Squamous Cell Carcinoma Models of Head and Neck Cancer
Authors: Bommireddy Ramireddy , Munoz Luis E. , Kumari Anita , Huang Lei , Fan Yijian , Monterroza Lenore , Pack Christopher D. , Ramachandiran Sampath , Reddy Shaker J.C. , Kim Janet , Chen Zhuo G. , Saba Nabil F. , Shin Dong M. , Selvaraj Periasamy
Published: April 2020

Immune checkpoint inhibitor (ICI) immunotherapy improved the survival of head and neck squamous cell carcinoma (HNSCC) patients. However, more than 80% of the patients are still resistant to this therapy. To test whether the efficacy of ICI therapy can be improved by vaccine-induced immunity, we investigated the efficacy of a tumor membrane-based vaccine immunotherapy in murine models of HNSCC. The tumors, grown subcutaneously, are used to prepare tumor membrane vesicles (TMVs). TMVs are then incorporated with glycolipid-anchored immunostimulatory molecules GPI-B7-1 and GPI-IL-12 by protein transfer to generate the TMV vaccine. This TMV vaccine inhibited tumor growth and improved the survival of mice challenged with SCCVII tumor cells. The tumor-free mice survived for several months, remained tumor-free, and were protected following a secondary tumor cell challenge, suggesting that the TMV vaccine induced an anti-tumor immune memory response. However, no synergy with anti-PD1 mAb was observed in this model. In contrast, the TMV vaccine was effective in inhibiting MOC1 and MOC2 murine oral cancer models and synergized with anti-PD1 mAb in extending the survival of tumor-bearing mice. These observations suggest that tumor tissue based TMV vaccines can be harnessed to develop an effective personalized immunotherapy for HNSCC that can enhance the efficacy of immune checkpoint inhibitors.