bluuebunny/crossref_metadata_2025 huggingface dataset:
Satisfactory outcomes after one‐stage revision anterior cruciate ligament reconstruction using rectangular tunnel technique
Authors: Okimura Shinichiro , Suzuki Tomoyuki , Ikeda Yasutoshi , Shiwaku Kousuke , Teramoto Atsushi
Published: October 2023

Purpose

One‐stage revision anterior cruciate ligament reconstruction (ACLR) with the anatomic rectangular tunnel (RT) technique using bone–patellar tendon–bone (BTB) grafts results in anatomically precise tunnel placement and secure graft fixation. This study evaluated knee joint laxity and clinical outcomes in terms of femoral tunnel overlap. It was hypothesised that there would be no significant differences in knee joint laxity or clinical outcomes regardless of femoral tunnel overlap.

Methods

Between 2012 and 2021, a single surgeon conducted 196 one‐stage revision ACLRs with the RT technique using BTB grafts. Patients were divided based on the presence of femoral tunnel overlap. Knee joint laxity was evaluated using the Lachman test, pivot shift test, and side‐to‐side difference measured with a KT‐1000 arthrometer. Clinical outcomes were assessed using the Lysholm score, Knee Injury and Osteoarthritis Outcome Score (KOOS), and International Knee Documentation Committee (IKDC) Knee Examination Form 2000. Knee joint laxity and clinical outcomes were compared between groups after a median follow‐up of 2.5 years (range 2.0–8.0).

Results

The study included 30 and 73 patients in the overlap and non‐overlap groups, respectively. No significant differences were observed in the results of the Lachman test, pivot shift test, or KT‐1000 arthrometer as well as in the Lysholm, KOOS, or IKDC scores between the two groups. Based on the IKDC scores, all patients were graded as normal or nearly normal.

Conclusion

One‐stage revision ACLR with the RT technique using BTB grafts improved knee joint laxity and had favourable clinical outcomes regardless of femoral tunnel overlap. To achieve optimal results in one‐stage revision ACLR, it is crucial to create a tunnel within the anatomical attachment area and ensure proper graft fixation and tensioning.

Level of evidence

III.
Legality rating and corporate efficiency: evidence from a conditional nonparametric frontier analysis
Authors: DeBenedetto Marco A. , Giuranno Michele G. , Mastromarco Camilla , Ravenda Diego , Toma Pierluigi
Published: October 2023

Promoting legality and productivity is a central issue in modern economies. In this paper, we investigate the implications of a public policy that aims at achieving these goals through the adoption of the so-called legality rating (LR). The latter reduces corporate risk uncertainty by abating asymmetric information between companies that use good legal, fiscal and ethical practices and the credit system. We match companies whose legal and ethical practices have been certified by the Italian government—through the assignment of the LR—with a sample of unrated firms, and apply recent advances in nonparametric frontier analysis to assess the economic performance. We highlight a positive relationship between legality rating and firms’ production efficiency. Our findings also show that legality rating policy is an effective tool to enhance inter-regional technological catching-up of businesses.
Regulation of renal nitric oxide and eNOS/iNOS expression by tadalafil participates in the mitigation of amphotericin B–induced renal injury: Down-regulation of NF-κB/iNOS/caspase-3 signaling
Authors: Abdel-Rahman Doaa M. , Messiha Basim Anwar Shehata , Ali Fares E.M. , Azouz Amany A.
Published: October 2023

Amphotericin B (AmB)–induced acute kidney injury (AKI) is a common health problem having an undesirable impact on its urgent therapeutic utility for fatal systemic fungal infections. Tadalafil (TAD), a phosphodiesterase-5 (PDE-5) inhibitor, has been observed to have a wide range of pharmacological actions, including nephroprotection. The study’s objective was to examine the possible underlying protective mechanism of TAD against AmB-induced nephrotoxicity. Experimentally, animals were divided randomly into four groups: control, TAD (5 mg/kg/day; p.o.), AmB (18.5 mg/kg/day; i.p.), and TAD+AmB groups. Sera and tissue samples were processed for biochemical, molecular, and histological analyses. The biochemical investigations showed that TAD significantly ameliorated the increase of kidney function biomarkers (creatinine, urea, CysC, KIM-1) in serum, renal nitric oxide (NO), lipid peroxidation (MDA), and inflammatory cytokines (TNF-α, IL-6) in AmB-treated rats. Meanwhile, TAD significantly retarded AmB-induced decrease in serum magnesium, sodium, potassium, and renal glutathione content. Molecular analysis revealed that TAD reduced AmB-induced imbalance in the protein expression of eNOS/iNOS, which explains its regulatory effect on renal NO content. These results were also supported by the down-regulation of nuclear NF-κB p65 and cleaved caspase-3 protein expressions, as well as the improvement of histological features by TAD in AmB-treated rats. Therefore, it can be suggested that TAD could be a promising candidate for renoprotection against AmB-induced AKI. That could be partly attributed to its regulatory effect on renal eNOS/iNOS balance and NO, the inhibition of NF-κB p65 nuclear translocation, its downstream inflammatory cytokines and iNOS, and ultimately the inhibition of caspase-3-induced renal apoptosis.
Intra-arterial peptide-receptor radionuclide therapy for neuro-endocrine tumour liver metastases: an in-patient randomised controlled trial (LUTIA)
Authors: Ebbers S. C. , Barentsz M. W. , de Vries-Huizing D. M. V. , Versleijen M. W. J. , Klompenhouwer E. G. , Tesselaar M. E. T. , Stokkel M. P. M. , Brabander T. , Hofland J. , Moelker A. , van Leeuwaarde R. S. , Smits M. L. J. , Braat A. J. A. T. , Lam M. G. E. H.
Published: October 2023

Purpose

Peptide receptor radionuclide therapy (PRRT) using [177Lu]Lu-DOTATATE has been shown to effectively prolong progression free survival in grade 1–2 gastroenteropancreatic neuroendocrine tumours (GEP-NET), but is less efficacious in patients with extensive liver metastases. The aim was to investigate whether tumour uptake in liver metastases can be enhanced by intra-arterial administration of [177Lu]Lu-DOTATATE into the hepatic artery, in order to improve tumour response without increasing toxicity.

Methods

Twenty-seven patients with grade 1–2 GEP-NET, and bi-lobar liver metastases were randomized to receive intra-arterial PRRT in the left or right liver lobe for four consecutive cycles. The contralateral liver lobe and extrahepatic disease were treated via a “second-pass” effect and the contralateral lobe was used as the control lobe. Up to three metastases (> 3 cm) per liver lobe were identified as target lesions at baseline on contrast-enhanced CT. The primary endpoint was the tumour-to-non-tumour (T/N) uptake ratio on the 24 h post-treatment [177Lu]Lu-SPECT/CT after the first cycle. This was calculated for each target lesion in both lobes using the mean uptake. T/N ratios in both lobes were compared using paired-samples t-test.

Findings

After the first cycle, a non-significant difference in T/N uptake ratio was observed: T/NIA = 17·4 vs. T/Ncontrol = 16·2 (p = 0·299). The mean increase in T/N was 17% (1·17; 95% CI [1·00; 1·37]). Of all patients, 67% (18/27) showed any increase in T/N ratio after the first cycle.

Conclusion

Intra-arterial [177Lu]Lu-DOTATATE is safe, but does not lead to a clinically significant increase in tumour uptake.
Impact of simulated reduced injected dose on the assessment of amyloid PET scans
Authors: Young Peter , Heeman Fiona , Axelsson Jan , Collij Lyduine E. , Hitzel Anne , Sanaat Amirhossein , Niñerola-Baizan Aida , Perissinotti Andrés , Lubberink Mark , Frisoni Giovanni B. , Zaidi Habib , Barkhof Frederik , Farrar Gill , Baker Suzanne , Gispert Juan Domingo , Garibotto Valentina , Rieckmann Anna , Schöll Michael ,   on behalf of the AMYPAD consortium
Published: October 2023

Purpose

To investigate the impact of reduced injected doses on the quantitative and qualitative assessment of the amyloid PET tracers [18F]flutemetamol and [18F]florbetaben.

Methods

Cognitively impaired and unimpaired individuals (N = 250, 36% Aβ-positive) were included and injected with [18F]flutemetamol (N = 175) or [18F]florbetaben (N = 75). PET scans were acquired in list-mode (90–110 min post-injection) and reduced-dose images were simulated to generate images of 75, 50, 25, 12.5 and 5% of the original injected dose. Images were reconstructed using vendor-provided reconstruction tools and visually assessed for Aβ-pathology. SUVRs were calculated for a global cortical and three smaller regions using a cerebellar cortex reference tissue, and Centiloid was computed. Absolute and percentage differences in SUVR and CL were calculated between dose levels, and the ability to discriminate between Aβ- and Aβ + scans was evaluated using ROC analyses. Finally, intra-reader agreement between the reduced dose and 100% images was evaluated.

Results

At 5% injected dose, change in SUVR was 3.72% and 3.12%, with absolute change in Centiloid 3.35CL and 4.62CL, for [18F]flutemetamol and [18F]florbetaben, respectively. At 12.5% injected dose, percentage change in SUVR and absolute change in Centiloid were < 1.5%. AUCs for discriminating Aβ- from Aβ + scans were high (AUC ≥ 0.94) across dose levels, and visual assessment showed intra-reader agreement of > 80% for both tracers.

Conclusion

This proof-of-concept study showed that for both [18F]flutemetamol and [18F]florbetaben, adequate quantitative and qualitative assessments can be obtained at 12.5% of the original injected dose. However, decisions to reduce the injected dose should be made considering the specific clinical or research circumstances.
Influence of deep gaps on laminar–turbulent transition in two-dimensional boundary layers at subsonic Mach numbers
Authors: Risius Steffen , Costantini Marco , Klein Christian
Published: October 2023

Systematic experimental investigations on the influence of deep gaps on the location of laminar–turbulent transition are reported. The tests were conducted in the Cryogenic Ludwieg–Tube Göttingen, a blow-down wind tunnel with good flow quality, at eight different unit Reynolds numbers ranging from $$Re_1 = {17.5\,\,\times 10^{6}\,\mathrm{{m}^{-1}}}$$

R

e
1

=

17.5

×

10
6

m

-
1

to $$ 80\,\times\,10^{6}\,\mathrm{m}^{-1}$$

80

×

10
6

m

-
1

, three Mach numbers, $$M= 0.35$$

M
=
0.35

, 0.50 and 0.65, and various pressure gradients. A flat-plate configuration, the extended two-dimensional wind tunnel model PaLASTra was modified in order to allow the installation of gaps with nominal widths of $$30$$

30

 $$\upmu $$
μ
m, $$100$$

100

 $$\upmu $$
μ
m and $$200$$

200

 $$\upmu $$
μ
m and a depth of $$d = {9\,\mathrm{mm}}$$

d
=

9

mm

. A maximum Reynolds number based on the gap width $$Re_{w} = Re_1 \cdot w \approx {16{,}000}$$

R

e
w

=
R

e
1

·
w
≈

16
,
000

was reached. Transition Reynolds numbers ranging from $$Re_{tr}\approx $$

R

e

tr

≈

1 × 106 to 11 × 106 were measured, as a function of gap width, pressure gradient and Mach and Reynolds number. This systematic investigation facilitates a linear approximation of $$Re_{tr}$$

R

e

tr

dependent on the boundary layer shape factor $$H_{12}$$

H
12

for various flow conditions and gap widths. It was therefore possible to conduct an investigation of $$Re_{tr}$$

R

e

tr

depending on $$Re_{1}$$

R

e
1

and the relative change of the transition location depending on the gap width w. Incompressible linear stability analysis was used to calculate amplification rates of Tollmien–Schlichting waves and determine critical N-factors by correlation with measured transition locations. The change in the critical N-factor $$\varDelta N$$

Δ
N

by installation of the gap is investigated as a function of w and $$Re_w$$

R

e
w

. It was found that a gap width of 30 $$\upmu $$
μ
m reduces the critical N-factors in the range of $$\varDelta N \approx 0.5 \pm 0.25$$

Δ
N
≈
0.5
±
0.25

, while gap widths of 100 $$\upmu $$
μ
m and 200 $$\upmu $$
μ
m reduce the critical N-factor in the range of $$\varDelta N \approx 1.5 \pm 1$$

Δ
N
≈
1.5
±
1

. Interestingly, an increase in gap width from 100 to 200 $$\upmu $$
μ
m was not found to induce smaller transition Reynolds numbers or reduced N-factors, which might be due to resonance effects.
High-precision interferometric measurement of slow and fast temperature changes in static fluid and convective flow
Authors: Ge Xinyang , Zielińska Joanna A. , Maldonado Sergio
Published: October 2023

We explore the strengths and limitations of using a standard Michelson interferometer to sample line-of-sight-averaged temperature in water via two experimental setups: slow-varying temperature in static fluid and fast temperature variations in convective flow. The high precision of our measurements (a few mK) is enabled by the fast response time and high sensitivity of the interferometer to minute changes in the refractive index of water caused by temperature variations. These features allow us to detect the signature of fine fluid dynamical patterns in convective flow in a fully non-intrusive manner. For example, we are able to observe an asymmetry in the rising thermal plume (i.e., an asynchronous arrival of two counter-rotating vortices at the measurement location), which is not possible to resolve with more traditional (and invasive) techniques, such as RTD (Resistance Temperature Detector) sensors. These findings, and the overall reliability of our method, are further corroborated by means of Particle Image Velocimetry and Large Eddy Simulations. While this method presents inherent limitations (mainly stemming from the line-of-sight-averaged nature of its results), its non-intrusiveness and robustness, along with the ability to readily yield real-time, highly accurate measurements, render this technique very attractive for a wide range of applications in experimental fluid dynamics.
The neuropathology of intimate partner violence
Authors: Dams-O’Connor Kristen , Seifert Alan C. , Crary John F. , Delman Bradley N. , Del Bigio Marc R. , Kovacs Gabor G. , Lee Edward B. , Nolan Amber L. , Pruyser Ariel , Selmanovic Enna , Stewart William , Woodoff-Leith Emma , Folkerth Rebecca D.
Published: October 2023

Lifelong brain health consequences of traumatic brain injury (TBI) include the risk of neurodegenerative disease. Up to one-third of women experience intimate partner violence (IPV) in their lifetime, often with TBI, yet remarkably little is known about the range of autopsy neuropathologies encountered in IPV. We report a prospectively accrued case series from a single institution, the New York City Office of Chief Medical Examiner, evaluated in partnership with the Brain Injury Research Center of Mount Sinai, using a multimodal protocol comprising clinical history review, ex vivo imaging in a small subset, and comprehensive neuropathological assessment by established consensus protocols. Fourteen brains were obtained over 2 years from women with documented IPV (aged 3rd–8th decade; median, 4th) and complex histories including prior TBI in 6, nonfatal strangulation in 4, cerebrovascular, neurological, and/or psychiatric conditions in 13, and epilepsy in 7. At autopsy, all had TBI stigmata (old and/or recent). In addition, white matter regions vulnerable to diffuse axonal injury showed perivascular and parenchymal iron deposition and microgliosis in some subjects. Six cases had evidence of cerebrovascular disease (lacunes and/or chronic infarcts). Regarding neurodegenerative disease pathologies, Alzheimer disease neuropathologic change was present in a single case (8th decade), with no chronic traumatic encephalopathy neuropathologic change (CTE-NC) identified in any. Findings from this initial series then prompted similar exploration in an expanded case series of 70 archival IPV cases (aged 2nd–9th decade; median, 4th) accrued from multiple international institutions. In this secondary case series, we again found evidence of vascular and white matter pathologies. However, only limited neurodegenerative proteinopathies were encountered in the oldest subjects, none meeting consensus criteria for CTE-NC. These observations from this descriptive exploratory study reinforce a need to consider broad co-morbid and neuropathological substrates contributing to brain health outcomes in the context of IPV, some of which may be potentially modifiable.
Agreement of cerebrospinal fluid biomarkers and amyloid-PET in a multicenter study
Authors: Guillén Núria , Contador José , Buongiorno Mariateresa , Álvarez Ignacio , Culell Natalia , Alcolea Daniel , Lleó Alberto , Fortea Juan , Piñol-Ripoll Gerard , Carnes-Vendrell Anna , Lourdes Ispierto María , Vilas Dolores , Puig-Pijoan Albert , Fernández-Lebrero Aida , Balasa Mircea , Sánchez-Valle Raquel , Lladó Albert
Published: October 2023

Core Alzheimer’s disease (AD) cerebrospinal fluid (CSF) biomarkers have shown incomplete agreement with amyloid-positron emission tomography (PET). Our goal was to analyze the agreement between AD CSF biomarkers and amyloid-PET in a multicenter study. Retrospective multicenter study (5 centers). Participants who underwent both CSF biomarkers and amyloid-PET scan within 18 months were included. Clinical diagnoses were made according to latest diagnostic criteria by the attending clinicians. CSF Amyloid Beta1-42 (Aβ1-42, A), phosphorliated tau 181 (pTau181, T) and total tau (tTau, N) biomarkers were considered normal (−) or abnormal ( +) according to cutoffs of each center. Amyloid-PET was visually classified as positive/negative. Agreement between CSF biomarkers and amyloid-PET was analyzed by overall percent agreement (OPA). 236 participants were included (mean age 67.9 years (SD 9.1), MMSE score 24.5 (SD 4.1)). Diagnoses were mild cognitive impairment or dementia due to AD (49%), Lewy body dementia (22%), frontotemporal dementia (10%) and others (19%). Mean time between tests was 5.1 months (SD 4.1). OPA between single CSF biomarkers and amyloid-PET was 74% for Aβ1-42, 75% for pTau181, 73% for tTau. The use of biomarker ratios improved OPA: 87% for Aβ1-42/Aβ1-40 (n = 155), 88% for pTau181/Aβ1-42 (n = 94) and 82% for tTau/Aβ1-42 (n = 160). A + T + N + cases showed the highest agreement between CSF biomarkers and amyloid-PET (96%), followed by A-T-N- cases (89%). Aβ1-42/Aβ1-40 was a better marker of cerebral amyloid deposition, as identified by amyloid tracers, than Aβ1-42 alone. Combined biomarkers in CSF predicted amyloid-PET result better than single biomarkers.
A lighthouse to enhance the quality of life in the Nile River basin
Authors: Batisha Ayman
Published: October 2023

The future of large rivers is related to regional cooperation programs for the sustainable development of water and related resources in great river basins. The objective of this article is to present how roadmaps can be utilized for both building up sustainable development for the Nile River, and supporting the integration of national and regional development strategies in Egypt and other Nile basin countries. The strategic objective is to highlight a wide range of sustainable freshwater pathways for an inclusive, peaceful, and sustainable future for all. In particular, it focuses on generating innovative water solutions, actions, and practices that enhance water exploitation in large river basins, transboundary shared rivers, and other transboundary shared water resources. The roadmapping approach is adopted and developed widely in the science, technology, and innovation community. The paper concentrates on roadmapping as an important tool that promotes sound planning of sustainability of large rivers, and in particular, transboundary shared rivers. The roadmap analyzes the benefits of planning shared water cooperation that extends to ecological, economic, and political features, and also beyond the river for the benefit of all, and identifies at an early stage the actions needed to manage the associated technology and environmental risk. The roadmap includes the key water resources governance characteristics, geographical scope, climate change (CC), and its impact, member states, organizational structure, decision-making, data information sharing, monitoring, the role of multiple stakeholders, financing, legal basis, dispute resolution, and benefit sharing. Therefore, a roadmap for the Nile River can help raise productivity and support faster growth, if considered as a lighthouse for national development strategies. Roadmap for the Nile River proposes windows of opportunity and is a vital milestone for cooperation, peace, stability, joint investment, and prosperity. With possible benefits exceeding those derived from the river itself, a roadmap for the Nile River can catalyze strengthened cooperation and greater regional ecological, economic, social, and political integration. The paper concludes that the roadmap for the Nile River sustainability (NRS) is a promising model for assuring the sustainability of transboundary shared large rivers.
N6-methyladenosine modification of OIP5-AS1 promotes glycolysis, tumorigenesis, and metastasis of gastric cancer by inhibiting Trim21-mediated hnRNPA1 ubiquitination and degradation
Authors: Xie Rongjun , Liu Longfei , Lu Xianzhou , He Chengjian , Yao Hongyi , Li Guoxin
Published: October 2023

Background

Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) has been demonstrated to play vital roles in development and progression of tumors such as gastric cancer (GC). However, the detailed molecular mechanism of OIP5-AS1 has not been completely elucidated. Our study aimed to investigate the role and the epigenetic regulation mechanism of OIP5-AS1 in GC.

Methods

OIP5-AS1 expression in GC tissues was detected by RT-qPCR. Loss- and gain-of-function experiments were conducted to assess the biological function of OIP5-AS1 in vitro and in vivo. The interaction of OIP5-AS1 with insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) or heterogeneous nuclear nucleoprotein A1 (hnRNPA1) was verified by bioinformatics analysis, RNA pull-down assays, and RNA immunoprecipitation assays.

Results

In this study, we identified that OIP5-AS1 is specifically overexpressed in GC tumor tissues and cell lines and correlated with a poor prognosis. The loss of OIP5-AS1 suppressed the proliferation, migration, invasion, epithelial–mesenchymal transition (EMT), and glycolysis of GC cells, but the ectopic expression of OIP5-AS1 had the opposite impact. Meanwhile, knockdown of OIP5-AS1 inhibited tumor growth in patient-derived xenograft models, as well as repressed tumor metastasis. Mechanistically, IGF2BP3 could bind to OIP5-AS1 by N6-methyladenosine (m6A) modification sites on OIP5-AS1, thereby stabilizing OIP5-AS1. Moreover, OIP5-AS1 prevented Trim21-mediated ubiquitination and degradation of hnRNPA1, stabilizing hnRNPA1 protein and promoting the malignant progression of GC by regulating PKM2 signaling pathway.

Conclusions

In conclusion, this study highlighted that OIP5-AS1 is an oncogenic m6A-modified long non-coding RNA (lncRNA) in GC and that IGF2BP3/OIP5-AS1/hnRNPA1 axis may provide a potential diagnostic or prognostic target for GC.
Effective Neutralizing Antibody Response Against SARS-CoV-2 Virus and Its Omicron BA.1 Variant in Fully Vaccinated Hematological Patients
Authors: De Novellis Danilo , Folliero Veronica , Giudice Valentina , Pezzullo Luca , Sanna Giuseppina , Fontana Raffaele , Guariglia Roberto , Zannella Carla , Mettivier Laura , Ferrara Idalucia , Boccia Giovanni , Buonanno Maria Teresa , Martorelli Maria Carmen , Luponio Serena , Crudele Andrea , Pagliano Pasquale , Sessa Anna Maria , Velino Francesca , Langella Maddalena , Manzin Aldo , Galdiero Massimiliano , Selleri Carmine , Franci Gianluigi , Serio Bianca
Published: October 2023

SARS-CoV-2 and its variants cause CoronaVIrus Disease 19 (COVID-19), a pandemic disease. Hematological malignancies increase susceptibility to severe COVID-19 due to immunosuppression. Anti-SARS-CoV-2 neutralizing antibodies protect against severe COVID-19. This retrospective real-life study aimed to evaluate seropositivity and neutralizing antibody rates against SARS-CoV-2 and its Omicron BA.1 variant in hematological patients. A total of 106 patients with different hematologic malignancies, who have mostly received three or more vaccine doses (73%), were included in this study. Serum was collected between May and June 2022. The primary endpoint was anti-SARS-CoV-2 antibody response against ancestral (wild type; wt) and Omicron BA.1 virus, defined as a neutralizing antibody titer ≥ 1:10. Adequate neutralizing antibody response was observed in 75 (71%) and 87 (82%) of patients for wt and Omicron BA.1 variants, respectively.

However, patients with B-cell lymphoproliferative disorders and/or those treated with anti-CD20 monoclonal antibodies in the prior 12 months showed a lower seropositivity rate compared to other patients against both Omicron BA.1 variant (73% vs 91%; P = 0.02) and wt virus (64% vs 78%; P = 0.16). Our real-life experience confirmed that full vaccination against SARS-CoV-2 induces adequate neutralizing antibody protection for both the wt virus and Omicron BA.1 variants, even in hematological frail patients. However, protective measures should be maintained in hematological patients, especially those with B-cell lymphoproliferative diseases treated with anti-CD20 monoclonal antibodies, because these subjects could have a reduced neutralizing antibody production.
Software defect prediction using a bidirectional LSTM network combined with oversampling techniques
Authors: Khleel Nasraldeen Alnor Adam , Nehéz Károly
Published: October 2023

Software defects are a critical issue in software development that can lead to system failures and cause significant financial losses. Predicting software defects is a vital aspect of ensuring software quality. This can significantly impact both saving time and reducing the overall cost of software testing. During the software defect prediction (SDP) process, automated tools attempt to predict defects in the source codes based on software metrics. Several SDP models have been proposed to identify and prevent defects before they occur. In recent years, recurrent neural network (RNN) techniques have gained attention for their ability to handle sequential data and learn complex patterns. Still, these techniques are not always suitable for predicting software defects due to the problem of imbalanced data. To deal with this problem, this study aims to combine a bidirectional long short-term memory (Bi-LSTM) network with oversampling techniques. To establish the effectiveness and efficiency of the proposed model, the experiments have been conducted on benchmark datasets obtained from the PROMISE repository. The experimental results have been compared and evaluated in terms of accuracy, precision, recall, f-measure, Matthew’s correlation coefficient (MCC), the area under the ROC curve (AUC), the area under the precision-recall curve (AUCPR) and mean square error (MSE). The average accuracy of the proposed model on the original and balanced datasets (using random oversampling and SMOTE) was 88%, 94%, And 92%, respectively. The results showed that the proposed Bi-LSTM on the balanced datasets (using random oversampling and SMOTE) improves the average accuracy by 6 and 4% compared to the original datasets. The average F-measure of the proposed model on the original and balanced datasets (using random oversampling and SMOTE) were 51%, 94%, And 92%, respectively. The results showed that the proposed Bi-LSTM on the balanced datasets (using random oversampling and SMOTE) improves the average F-measure by 43 and 41% compared to the original datasets. The experimental results demonstrated that combining the Bi-LSTM network with oversampling techniques positively affects defect prediction performance in datasets with imbalanced class distributions.
Employment protection and labour productivity growth in the EU: skill-specific effects during and after the Great Recession
Authors: Fedotenkov Igor , Kvedaras Virmantas , Sanchez-Martinez Miguel
Published: October 2023

Does employment protection affect sectoral productivity growth differently during crises and recovery periods? This paper sheds light into this question by investigating the relationship between employment protection legislation (EPL hereafter) and sectoral labour productivity growth in the EU in the context of the Great Recession. We consider the crisis and recovery periods, evaluate the relevance of both levels and changes in EPL for productivity growth, and explore the conditioning role played by sectoral differences in terms of cumulativeness of knowledge as well as the skills of the labour force, captured by different levels of education. We find that stricter labour protection reduces labour productivity growth in sectors with a large share of workers with tertiary education, whereas this effect is negligible or positive in sectors where workers with secondary or only primary education are more prevalent (such as agriculture, mining and quarrying). We attribute this to a more intensive labour hoarding in the former, as EPL strengthens labour hoarding in sectors that rely on firm-specific knowledge accumulation and skilled human capital that are difficult to substitute with physical capital. Whereas it is simple to dismiss (and to find later) unskilled employees. They not only can be substituted more easily with capital, but also the costs of their firing are lower, they are overrepresented among workers holding temporary contracts, and they might be unequally informed and able to exercise their rights. This leads to low (if any) labour hoarding and little impact of EPL on labour productivity in such sectors. We also document that the negative effect is prominent only during the crisis, and an increase in the stringency of EPL over an extended period stimulates employers to substitute labour with investments in physical and knowledge capital.
The landscape of data and AI documentation approaches in the European policy context
Authors: Micheli Marina , Hupont Isabelle , Delipetrev Blagoj , Soler-Garrido Josep
Published: October 2023

Nowadays, Artificial Intelligence (AI) is present in all sectors of the economy. Consequently, both data-the raw material used to build AI systems- and AI have an unprecedented impact on society and there is a need to ensure that they work for its benefit. For this reason, the European Union has put data and trustworthy AI at the center of recent legislative initiatives. An important element in these regulations is transparency, understood as the provision of information to relevant stakeholders to support their understanding of AI systems and data throughout their lifecycle. In recent years, an increasing number of approaches for documenting AI and datasets have emerged, both within academia and the private sector. In this work, we identify the 36 most relevant ones from more than 2200 papers related to trustworthy AI. We assess their relevance from the angle of European regulatory objectives, their coverage of AI technologies and economic sectors, and their suitability to address the specific needs of multiple stakeholders. Finally, we discuss the main documentation gaps found, including the need to better address data innovation practices (e.g. data sharing, data reuse) and large-scale algorithmic systems (e.g. those used in online platforms), and to widen the focus from algorithms and data to AI systems as a whole.
Generative AI models should include detection mechanisms as a condition for public release
Authors: Knott Alistair , Pedreschi Dino , Chatila Raja , Chakraborti Tapabrata , Leavy Susan , Baeza-Yates Ricardo , Eyers David , Trotman Andrew , Teal Paul D. , Biecek Przemyslaw , Russell Stuart , Bengio Yoshua
Published: October 2023

The new wave of ‘foundation models’—general-purpose generative AI models, for production of text (e.g., ChatGPT) or images (e.g., MidJourney)—represent a dramatic advance in the state of the art for AI. But their use also introduces a range of new risks, which has prompted an ongoing conversation about possible regulatory mechanisms. Here we propose a specific principle that should be incorporated into legislation: that any organization developing a foundation model intended for public use must demonstrate a reliable detection mechanism for the content it generates, as a condition of its public release. The detection mechanism should be made publicly available in a tool that allows users to query, for an arbitrary item of content, whether the item was generated (wholly or partly) by the model. In this paper, we argue that this requirement is technically feasible and would play an important role in reducing certain risks from new AI models in many domains. We also outline a number of options for the tool’s design, and summarize a number of points where further input from policymakers and researchers would be required.
Analysis of the capsular bend in posterior capsular opacification using anterior segment optical coherence tomography
Authors: Gamal El-Deen Asmaa M
Published: October 2023

Purpose

To investigate the link between the capsular bend and the morphological types and characteristics of posterior capsular opacification (PCO) using anterior segment optical coherence tomography.

Methods

Thirty eyes with PCO were examined, and three types of PCO were identified: pearl, fibrosis, and mixed. We assessed anterior capsular overlap, intraocular lens-capsule adhesion, and capsular bending. In addition to measuring the intraocular lens-posterior capsule distance and capsule bending angle (CBA), the PCO parameters (area, density, and score at 6-, 5-, and 3-mm intraocular lens optic regions) were recorded. The associations between capsular bend and PCO type and characteristics were investigated. A control group of 12 eyes without PCO was used to compare the study variables.

Results

With p values greater than 0.001, there was a statistically significant difference in the mean PCO area and score at the 6-, 5-, and 3-mm optic zones in different PCO types, with the pearl type having the highest value, followed by the mixed type, and finally the fibrosis type. The PCO group had a significantly higher mean CBA than the control group (P = 0.001). CBA was positively related to intraocular lens-posterior capsule distance, PCO area, and PCO score at the 6-, 5-, and 3-mm zones (P = 0.001). The receiver operating characteristic curve's cut-off point for CBA was 96.85° when comparing PCO cases to controls. Partial overlap and incomplete adhesion were statistically more common in the PCO eyes than in the control (P = 0.001, 0.003, respectively).

Conclusion

PCO types and CBA have a strong relationship with PCO score and intraocular lens-posterior capsule space. In PCO's eyes, CBA has a cut-off value of 96.85°.
Cerebrospinal fluid circulating tumour DNA genotyping and survival analysis in lung adenocarcinoma with leptomeningeal metastases
Authors: Bai Kaixuan , Chen Xin , Qi Xuejiao , Zhang Yu , Zou Yueli , Li Jian , Yu Lili , Li Yuanyuan , Jiang Jiajia , Yang Yi , Liu Yajing , Feng Shuanghao , Bu Hui
Published: October 2023

Purpose

The prognosis of patients with leptomeningeal metastasis (LM) remains poor. Circulating tumour DNA (ctDNA) has been proven to be abundantly present in cerebrospinal fluid (CSF); hence, its clinical implication as a biomarker needs to be further verified.

Methods

We conducted a retrospective study of 35 lung adenocarcinoma (LUAD) patients with LM, and matched CSF and plasma samples were collected from all patients. All paired samples underwent next-generation sequencing (NGS) of 139 lung cancer-associated genes. The clinical characteristics and genetic profiling of LM were analysed in association with survival prognosis.

Results

LM showed genetic heterogeneity, in which CSF had a higher detection rate of ctDNA (P = 0.003), a higher median mutation count (P < 0.0001), a higher frequency of driver mutations (P < 0.01), and more copy number variation (CNV) alterations (P < 0.001) than plasma. The mutation frequencies of the EGFR, TP53, CDKN2A, MYC and CDKN2B genes were easier to detect in CSF than in LUAD tissue (P < 0.05), possibly reflecting the underlying mechanism of LM metastasis. CSF ctDNA is helpful for analysing the mechanism of EGFR-TKI resistance. In cohort 1, which comprised patients who received 1/2 EGFR-TKIs before the diagnosis of LM, TP53 and CDKN2A were the most common EGFR-independent resistant mutations. In cohort 2, comprising those who progressed after osimertinib and developed LM, 7 patients (43.75%) had EGFR CNV detected in CSF but not plasma. Furthermore, patient characteristics and various genes were included for interactive survival analysis. Patients with EGFR-mutated LUAD (P = 0.042) had a higher median OS, and CSF ctDNA mutation with TERT (P = 0.013) indicated a lower median OS. Last, we reported an LM case in which CSF ctDNA dynamic changes were well correlated with clinical treatment.

Conclusions

CSF ctDNA could provide a more comprehensive genetic landscape of LM, indicating the potential metastasis-related and EGFR-TKI resistance mechanisms of LM patients. In addition, genotyping of CSF combined with clinical outcomes can predict the prognosis of LUAD patients with LM.
Urbanization related changes in lepidopteran community
Authors: Vähätalo Anssi V. , Pulli Ahti , Kulmala Topias , Marin Roosa , Haimi Jari
Published: October 2023

Urbanization displaces natural habitats with impervious surfaces and managed ornamental green infrastructure. This study compared the structure of lepidopteran community in an office campus cleared from forest to that in the remaining forest. For the comparison, we trapped 2,233 lepidopteran specimens of 56 species from an office campus and adjacent forest. The species richness of lepidopteran assemblage in the office campus was half of that in the forest and consisted primarily of the same species found in the forest. The abundance and biomass of Lepidoptera in the office campus was a quarter of that in the forest. The biomass and abundance of Lepidoptera decreased along with the impervious area within 100-meter radius around the traps and approached zero when impervious surfaces covered the area entirely. The specimens in the trapped lepidopteran assembly from the office campus were on average larger, indicating elevated mobility, than those caught from the forest. Our results support earlier studies concluding that fragmented urban landscape selects for large mobile species, which can feed on ornamental plants or can disperse between high-quality habitats within urban landscape. Green infrastructure with native plants, high-quality native habitats and their connectivity can maintain species-rich lepidopteran communities in urban landscapes.
Environmental potential of fungal insulation: a prospective life cycle assessment of mycelium-based composites
Authors: Alaux Nicolas , Vašatko Hana , Maierhofer Dominik , Saade Marcella Ruschi Mendes , Stavric Milena , Passer Alexander
Published: October 2023

Purpose

Bio-based insulation materials are one of the most promising solutions for reducing the environmental impacts of building envelopes. Among these materials, the environmental benefits of mycelium-based materials have merely been investigated, despite their promising technical and thermal properties. In this paper, we perform a first prospective cradle-to-grave life cycle assessment (LCA) of mycelium-based composite blocks.

Methods

An attributional cradle-to-gate LCA of the laboratory production of mycelium-based composites was first performed, including 11 environmental impact indicators. Then, scenarios were defined to scale up the technology to the level of industrial production, including the remaining life cycle modules to perform a cradle-to-grave analysis. Biogenic and metabolic carbon were considered by applying the static −1/+1 approach and following the current LCA standards. Future-oriented energy and transport mixes were also included as an additional scenario, systematically modifying both the foreground and background data. Finally, the industrially scaled-up technology and alternative insulation materials were compared with these future conditions (as applied to both materials).

Results and discussion

Considering climate change, the results are encouraging in comparison to those for traditional plastic insulation, but do not necessarily surpass those for other existing materials such as rock wool. However, trade-offs are observed in other indicators, for which mycelium-based composites tend to perform worse than traditional insulation materials. The industrial scale-up reduced impacts for most indicators, but a considerable trade-off was observed with regard to terrestrial ecotoxicity. The main driver for the remaining greenhouse gas (GHG) emissions was found to be the electricity use during the manufacturing phase. We consider the inclusion of the other life cycle stages as relevant, as this increased the GHG emissions by 10%. Limitations of the current LCA standards, however, are noted and discussed, especially regarding the cascading use of biogenic materials, and highlight the relevance of this case study.

Conclusions

Mycelium-based composites show a potential for future development, but careful attention should be paid to reducing electricity needs in their manufacturing process. Further improvements could also be made by using fast-growing biogenic materials as a substrate. In particular, we encourage researchers to include all of the life cycle stages in future studies, especially if biogenic emissions are considered.
Theoretical study on bubble dynamics under hybrid-boundary and multi-bubble conditions using the unified equation
Authors: Zhang A-Man , Li Shi-Min , Cui Pu , Li Shuai , Liu Yun-Long
Published: October 2023

This paper aims to use the unified bubble dynamics equation to investigate bubble behavior in complex scenarios involving hybrid free surface/wall boundaries and interactions between multiple bubbles. The effect of singularity movement on the unified equation’s form is analyzed after deriving the bubble pulsation equation using a moving point source and a dipole, followed by discussions on the effect of migration compressibility-related terms on the bubble dynamics. In addition, the present study accounts for the impact of hybrid boundaries, including crossed and parallel boundaries, by introducing a finite number of mirror bubbles for the former and an infinite number of mirror bubbles for the latter. Spark bubble experiments and numerical simulation are conducted to validate the present theory. The application of the unified equation in multi-bubble interactions is exemplified by computing a spherical bubble array containing more than 100 uniformly distributed cavitation bubbles under different boundary conditions. The bubble cluster-induced pressure peak can reach nearly two times or even higher than that of an individual bubble, highlighting the damage potential caused by cavitation bubble clusters.
Influence of surface cracking, anchor head profile, and anchor head size on cast-in headed anchors in geopolymer concrete
Authors: Karmokar Trijon , Moyheddin Alireza
Published: October 2023

In this study, the concrete cone capacity, concrete cone angle, and load–displacement response of cast-in headed anchors in geopolymer concrete are explored using numerical analyses. The concrete damaged plasticity (CDP) model in ABAQUS is used to simulate the behavior of concrete substrates. The tensile behavior of anchors in geopolymer concrete is compared with that in normal concrete as well as that predicted by the linear fracture mechanics (LFM) and concrete capacity design (CCD) models. The results show that the capacity of the anchors in geopolymer concrete is 30%–40% lower than that in normal concrete. The results also indicate that the CCD model overestimates the capacity of the anchors in geopolymer concrete, whereas the LFM model provides a much more conservative prediction. The extent of the difference between the predictions by the numerical analysis and those of the above prediction models depends on the effective embedment depth of the anchor and the anchor head size. The influence of concrete surface cracking on the capacity of the anchor is shown to depend on the location of the crack and the effective embedment depth. The influence of the anchor head profile on the tensile capacity of the anchors is found to be insignificant.
Staging TEVAR after FET — an exception or the rule?
Authors: Di Marco Luca , Nocera Chiara , Snaidero Silvia , Campanini Francesco , Buia Francesco , Lovato Luigi , Murana Giacomo , Pacini Davide
Published: October 2023

Purpose

Frozen elephant trunk (FET) was born as an ideal one-step procedure to treat complex arch and descending thoracic aorta pathology. It was then proved that it frequently needs reintervention, which can often be performed by thoracic endovascular aortic repair (TEVAR) extension since FET provides a safe proximal landing zone. We hereby describe our experience in TEVAR extension after FET, its main indications, technique, and outcomes.

Methods

Between 2007 and 2022, 371 patients underwent FET at our center. Of these, 119 needed TEVAR extension. Some required more than one TEVAR, with a total of 154 procedures. The preoperative characteristics, indications, and outcomes were analyzed retrospectively.

Results

Of 154 TEVAR procedures, 15 were performed in an urgent setting. Mean time from FET to TEVAR was 22,2 ± 28,73 months. Two patients died in the operating room; no others died during the hospital stay. Survival after 1, 2, 5, and 10 years was 96.2%, 93.9%, 90.1%, and 70.5% respectively. There was no statistically significant difference in the rates of TEVAR extension for patients in which a Thoraflex™ vs E-vita™ graft was used, nor for zone 2 vs zone 3 anastomosis and stent length.

Conclusion

Though TEVAR extension is often required after FET, it is a safe and effective procedure with excellent post-operative outcomes in the short-, mid-, and long-term and allows successful treatment of complex aortic pathologies. Rigorous and specialized follow-up after FET is central to identify the right moment to intervene.
A Novel Paired Somatosensory-Cerebellar Stimulation Induces Plasticity on Cerebellar-Brain Connectivity
Authors: Ginatempo Francesca , Manzo Nicoletta , Spampinato Danny A. , Loi Nicola , Burgio Francesca , Rothwell John C. , Deriu Franca
Published: October 2023

The cerebellum receives and integrates a large amount of sensory information that is important for motor coordination and learning. The aim of the present work was to investigate whether peripheral nerve and cerebellum paired associative stimulation (cPAS) could induce plasticity in both the cerebellum and the cortex. In a cross-over design, we delivered right median nerve electrical stimulation 25 or 10 ms before applying transcranial magnetic stimulation over the cerebellum. We assessed changes in motor evoked potentials (MEP), somatosensory evoked potentials (SEP), short-afferent inhibition (SAI), and cerebellum-brain inhibition (CBI) immediately, and 30 min after cPAS. Our results showed a significant reduction in CBI 30 minutes after cPAS, with no discernible changes in MEP, SEP, and SAI. Notably, cPAS10 did not produce any modulatory effects on these parameters. In summary, cPAS25 demonstrated the capacity to induce plasticity effects in the cerebellar cortex, leading to a reduction in CBI. This novel intervention may be used to modulate plasticity mechanisms and motor learning in healthy individuals and patients with neurological conditions.