bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence, Knowledge and Awareness of Pelvic Floor Disorder among Pregnant Women in a Tertiary Centre, Malaysia
Authors: Nur Farihan Mukhtar , Ng Beng Kwang , Phon Su Ee , Nor Azlin Mohamed Ismail , Nur Azurah Abdul Ghani , Lim Pei Shan
Published: July 2022

Pelvic floor disorders are common and of concern, as the majority of maternity healthcare providers seldom discuss this issue with patients compared to other antenatal issues. The aim of this study is to determine the prevalence and to assess the knowledge and awareness of pelvic floor disorder (PFD) among pregnant women in a tertiary centre in Malaysia. We also aim to assess the association between women’s risk factors regarding their knowledge and awareness of pelvic floor disorder so that primary prevention strategies can be planned, initiated and implemented in the future. This is a cross-sectional study with a total of four hundred twenty-four pregnant women that were recruited over a 6-month duration from May to November 2017 in a tertiary centre in Malaysia. The Pelvic Floor Distress Inventory (PFDI-20) was used to evaluate pelvic floor distress symptoms during pregnancy, namely urinary incontinence, pelvic organ prolapse and faecal incontinence. A validated Prolapse and Incontinence Knowledge Questionnaire (PIKQ), which consists of 24 items, was used to assess respondents’ knowledge about urinary incontinence (UI) and pelvic organ prolapse (POP). A total of 450 participants were approached, but 424 participants agreed to participate, showing a response rate of 94.3%. The median age was 31.5 years old, and 33.3% were primiparity. Overall, 46.1% of pregnant women had at least one symptom of pelvic floor disorder during pregnancy. Of these, 62.3% experienced urinary incontinence, 41.1% experienced symptoms of pelvic organ prolapse, and 37.8% experienced symptoms of faecal incontinence. The overall median score of PIKQ was 12.0 (8.0, 17.0). The median score for PIKQ—UI was 7.0 (5.0, 9.0) and the median score for PIKQ—POP was 6.0 (4.0, 8.0). There were 341 (80.4%) pregnant women that had a low level of knowledge in UI, and 191 (45.0%) had a low level of knowledge in POP. Having a tertiary level of education and receiving antenatal specialist care were both associated with better proficiency in both the PIKQ—UI (p < 0.001) and PIKQ—POP (p < 0.001) subscales. Pelvic floor disorder during pregnancy was common. A knowledge of pelvic floor disorder was lacking among pregnant women in this study. Having a tertiary education and receiving antenatal specialist care were both associated with better knowledge proficiency. This study hopefully serves as a basic platform for future educational programs to bridge the knowledge gaps in PFD among pregnant women.
Business Strategy and Environmental Information Disclosure Quality: Empirical Evidence from Chinese Heavy Pollution Listed Firms
Authors: Li Zhengguang , Li Ping , Zhao Xibo , Tu Ziying
Published: July 2022

Using the data of listed firms in China’s A-share heavy pollution industry between 2008 and 2020, based on organizational theory, this study examines the impact of prospector-type firms and defender-type firms on environmental information disclosure quality. Empirical evidence shows that prospector-type firms reduce environmental information disclosure quality, compared with defender-type firms. After a series of robustness tests, the conclusion is still valid. This paper tests the impact mechanism of business strategy on environmental information disclosure quality and finds that financing constraints play a mediating effect in the relationship between business strategy and environmental information disclosure quality. This paper enriches and expands the literature in the field of influencing factors of environmental information disclosure quality and economic consequences of business strategy. At the same time, the conclusion of this paper has important reference significance for regulators to formulate policies to improve environmental information disclosure quality according to the heterogeneity of business strategy.
The Impact of Pandemic-Related Life Stress on Internet Gaming: Social Cynicism and Gaming Motivation as Serial Mediators
Authors: Yan Elsie , Sun Rong-Wei , Wu Anise M. S. , Lai Daniel W. L. , Lee Vincent W. P.
Published: July 2022

A heightened interest in online gaming has emerged during COVID-19, and people have become increasingly vulnerable to internet gaming disorder (IGD). However, playing video games can also have a positive effect; gaming has been recognized as an efficient coping strategy. Currently, relatively little is understood about how online gaming can turn from an efficient coping strategy into an addiction disorder. This study investigated the mediating roles of social cynicism, escape and coping motives on the association between daily disruption during COVID-19 and IGD, seeking to reveal the underlying mechanism that influences the effects of gaming. A total of 203 participants in Hong Kong who reported having played electronic games during COVID-19 were surveyed. We conducted three hierarchical multiple regressions, then tested a serial mediation model using path analysis with structural equation modeling. The results revealed that escape motives significantly mediated the relationship between daily disruption related to COVID-19 and IGD, but no such effect was found for coping motives. Social cynicism alone was not a significant mediator, but social cynicism and escape motives in series mediated the relationship between daily disruption and IGD. These difference outcomes suggested different underlying mechanisms of escape and coping motives.
Adapting PCIT-Health for Telehealth Delivery: A Case Study
Authors: Domoff Sarah E. , Overton Mikaela M. , Borgen Aubrey L. , Niec Larissa N.
Published: July 2022

During the COVID-19 pandemic, children’s physical health and access to mental health resources have been two critical concerns. Parent-Child Interaction Therapy-Health (PCIT-Health) is a treatment model aimed at helping parents manage children’s general behavior and their behavior in obesogenic contexts (screen time and mealtime). Due to social distancing guidelines, PCIT-Health was adapted for remote delivery through video conferencing. In this article, we describe the experience of implementing virtual PCIT-Health with a family. The family’s progress through treatment is described, along with the challenges associated with remote service delivery and how those challenges were addressed. Progress through treatment was measured with questionnaires administered to caregivers and with observational measures of parent-child interactions. The results from these measures indicate that caregivers experienced a reduction in stress and improvements in their child’s behavior after PCIT-Health completion. They also reported engaging in healthier management of their child’s screen time and mealtime behaviors. As coded from observational assessments, parents increased their use of positive parenting practices. Telehealth-delivered PCIT-Health is a promising treatment modality for increasing parenting skills and improving child behavior.
A Review of the Presence of SARS-CoV-2 in Wastewater: Transmission Risks in Mexico
Authors: Herazo Mayerlin Sandoval , Nani Graciela , Zurita Florentina , Nakase Carlos , Zamora Sergio , Herazo Luis Carlos Sandoval , Betanzo-Torres Erick Arturo
Published: July 2022

The appearance of SARS-CoV-2 represented a new health threat to humanity and affected millions of people; the transmission of this virus occurs through different routes, and one of them recently under debate in the international community is its possible incorporation and spread by sewage. Therefore, the present work’s research objectives are to review the presence of SARS-CoV-2 in wastewater throughout the world and to analyze the coverage of wastewater treatment in Mexico to determine if there is a correlation between the positive cases of COVID-19 and the percentages of treated wastewater in Mexico as well as to investigate the evidence of possible transmission by aerosol sand untreated wastewater. Methodologically, a quick search of scientific literature was performed to identify evidence the presence of SARS-CoV-2 RNA (ribonucleic acid) in wastewater in four international databases. The statistical information of the positive cases of COVID-19 was obtained from data from the Health Secretary of the Mexican Government and the Johns Hopkins Coronavirus Resource Center. The information from the wastewater treatment plants in Mexico was obtained from official information of the National Water Commission of Mexico. The results showed sufficient evidence that SARS-CoV-2 remains alive in municipal wastewater in Mexico. Our analysis indicates that there is a low but significant correlation between the percentage of treated water and positive cases of coronavirus r = −0.385, with IC (95%) = (−0.647, −0.042) and p = 0.030; this result should be taken with caution because wastewater is not a transmission mechanism, but this finding is useful to highlight the need to increase the percentage of treated wastewater and to do it efficiently. In conclusions, the virus is present in untreated wastewater, and the early detection of SAR-CoV-2 could serve as a bioindicator method of the presence of the virus. This could be of great help to establish surveillance measures by zones to take preventive actions, which to date have not been considered by the Mexican health authorities. Unfortunately, wastewater treatment systems in Mexico are very fragile, and coverage is limited to urban areas and non-existent in rural areas. Furthermore, although the probability of contagion is relatively low, it can be a risk for wastewater treatment plant workers and people who are close to them.
Digital Health and Big Data Analytics: Implications of Real-World Evidence for Clinicians and Policymakers
Authors: Magalhães Teresa , Dinis-Oliveira Ricardo Jorge , Taveira-Gomes Tiago
Published: July 2022

Real world data (RWD) and real-world evidence (RWE) plays an increasingly important role in clinical research since scientific knowledge is obtained during routine clinical large-scale practice and not experimentally as occurs in the highly controlled traditional clinical trials. Particularly, the electronic health records (EHRs) are a relevant source of data. Nevertheless, there are also significant challenges in the correct use and interpretation of EHRs data, such as bias, heterogeneity of the population, and missing or non-standardized data formats. Despite the RWD and RWE recognized difficulties, these are easily outweighed by the benefits of ensuring the efficacy, safety, and cost-effectiveness in complement to the gold standards of the randomized controlled trial (RCT), namely by providing a complete picture regarding factors and variables that can guide robust clinical decisions. Their relevance can be even further evident as healthcare units develop more accurate EHRs always in the respect for the privacy of patient data. This editorial is an overview of the RWD and RWE major aspects of the state of the art and supports the Special Issue on “Digital Health and Big Data Analytics: Implications of Real-World Evidence for Clinicians and Policymakers” aimed to explore all the potential and the utility of RWD and RWE in offering insights on diseases in a broad spectrum.
Exploring the Chinese Public’s Perception of Omicron Variants on Social Media: LDA-Based Topic Modeling and Sentiment Analysis
Authors: Wang Han , Sun Kun , Wang Yuwei
Published: July 2022

The COVID-19 pandemic caused by SARS-CoV-2 is still raging. Similar to other RNA viruses, SARS-COV-2 is constantly mutating, which leads to the production of many infectious and lethal strains. For instance, the omicron variant detected in November 2021 became the leading strain of infection in many countries around the world and sparked an intense public debate on social media. The aim of this study is to explore the Chinese public’s perception of the omicron variants on social media. A total of 121,632 points of data relating to omicron on Sina Weibo from 0:00 27 November 2021 to 23:59:59 30 March 2022 (Beijing time) were collected and analyzed with LDA-based topic modeling and DLUT-Emotion ontology-based sentiment analysis. The results indicate that (1) the public discussion of omicron is based on five topics, including omicron’s impact on the economy, the omicron infection situation in other countries/regions, the omicron infection situation in China, omicron and vaccines and pandemic prevention and control for omicron. (2) From the 3 sentiment orientations of 121,632 valid Weibo posts, 49,402 posts were judged as positive emotions, accounting for approximately 40.6%; 47,667 were negative emotions, accounting for nearly 39.2%; and 24,563 were neutral emotions, accounting for about 20.2%. (3) The result of the analysis of the temporal trend of the seven categories of emotion attribution showed that fear kept decreasing, whereas good kept increasing. This study provides more insights into public perceptions of and attitudes toward emerging SARS-CoV-2 variants. The results of this study may provide further recommendations for the Chinese government, public health authorities, and the media to promote knowledge about SARS-CoV-2 variant pandemic-resistant messages.
Padel Match Analysis: Notational and Time-Motion Analysis during Official Italian Sub-Elite Competitions
Authors: Ungureanu Alexandru , Lupo Corrado , Brustio Paolo
Published: July 2022

Although performance analysis in padel represents a useful process to gain references about players’ technical and tactical behavior, most of the research was conducted in elite compared to the sub-elite competitions. Therefore, this study aimed to describe sub-elite competitions in order to enhance scientific knowledge for sub-elite athletes and technical staff. 4287 shots were analyzed within five areas (time-motion analysis, shots characteristics, errors, serve and points won). Effective playing time and work-to-rest ratio were lower than in elite competitions, while strokes per minute and total match duration were in line with it. Shots were mainly forehand volleys performed under the head, while volleys and smashes were more likely to end with a point in comparison with ground or wall shots. However, sub-elite winning pairs performed fewer volleys than the losing side and fewer errors on volleys. One serve out of five ended in errors (almost half were net errors); fewer errors during serve return shots represented an advantage for the winning pairs. Finally, 65% of the points scored were caused by unforced errors of opponents. This knowledge should help technical staff design specific training programs for sub-elite padel players.
Assessing the Relationship among Land Transfer, Fertilizer Usage, and PM2.5 Pollution: Evidence from Rural China
Authors: Guo Lili , Song Yuting , Tang Mengqian , Tang Jinyang , Dogbe Bright , Su Mengying , Li Houjian
Published: July 2022

Concern for environmental issues is a crucial component in achieving the goal of sustainable development of humankind. Different countries face various challenges and difficulties in this process, which require unique solutions. This study investigated the relationship between land transfer, fertilizer usage, and PM2.5 pollution in rural China from 2000 to 2019, considering their essential roles in agricultural development and overall national welfare. A cross section dependence test, unit root test, and cointegration test, among other methods, were used to test the panel data. A Granger causality test was used to determine the causal relationship between variables, and an empirical analysis of the impulse response and variance decomposition was carried out. The results show that the use of chemical fertilizers had a significant positive impact on PM2.5 pollution, but the impact of land transfer on PM2.5 pollution was negative. In addition, land transfer can reduce the use of chemical fertilizers through economies of scale, thus reducing air pollution. More specifically, for every 1% increase in fertilizer usage, PM2.5 increased by 0.17%, and for every 1% increase in land transfer rate, PM2.5 decreased by about 0.07%. The study on the causal relationship between land transfer, fertilizer usage, and PM2.5 pollution in this paper is helpful for exploring environmental change—they are supplements and innovations which are based on previous studies and provide policy-makers with a basis and inspiration for decision-making.
Antibacterial, Antibiofilm, and Antiviral Farnesol-Containing Nanoparticles Prevent Staphylococcus aureus from Drug Resistance Development
Authors: Ivanova Aleksandra , Ivanova Kristina , Fiandra Luisa , Mantecca Paride , Catelani Tiziano , Natan Michal , Banin Ehud , Jacobi Gila , Tzanov Tzanko
Published: July 2022

Multidrug antimicrobial resistance is a constantly growing health care issue associated with increased mortality and morbidity, and huge financial burden. Bacteria frequently form biofilm communities responsible for numerous persistent infections resistant to conventional antibiotics. Herein, novel nanoparticles (NPs) loaded with the natural bactericide farnesol (FSL NPs) are generated using high-intensity ultrasound. The nanoformulation of farnesol improved its antibacterial properties and demonstrated complete eradication of Staphylococcus aureus within less than 3 h, without inducing resistance development, and was able to 100% inhibit the establishment of a drug-resistant S. aureus biofilm. These antibiotic-free nano-antimicrobials also reduced the mature biofilm at a very low concentration of the active agent. In addition to the outstanding antibacterial properties, the engineered nano-entities demonstrated strong antiviral properties and inhibited the spike proteins of SARS-CoV-2 by up to 83%. The novel FSL NPs did not cause skin tissue irritation and did not induce the secretion of anti-inflammatory cytokines in a 3D skin tissue model. These results support the potential of these bio-based nano-actives to replace the existing antibiotics and they may be used for the development of topical pharmaceutic products for controlling microbial skin infections, without inducing resistance development.
New Frontiers in Monoclonal Antibodies for the Targeted Therapy of Acute Myeloid Leukemia and Myelodysplastic Syndromes
Authors: Gallazzi Marco , Ucciero Maghalie Anais Marie , Faraci Danilo Giuseppe , Mahmoud Abdurraouf Mokhtar , Al Essa Wael , Gaidano Gianluca , Mouhssine Samir , Crisà Elena
Published: July 2022

Acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS) represent an unmet clinical need whose prognosis is still dismal. Alterations of immune response play a prominent role in AML/MDS pathogenesis, revealing novel options for immunotherapy. Among immune system regulators, CD47, immune checkpoints, and toll-like receptor 2 (TLR2) are major targets. Magrolimab antagonizes CD47, which is overexpressed by AML and MDS cells, thus inducing macrophage phagocytosis with clinical activity in AML/MDS. Sabatolimab, an inhibitor of T-cell immunoglobulin and mucin domain-containing protein 3 (TIM3), which disrupts its binding to galectin-9, has shown promising results in AML/MDS, enhancing the effector functions of lymphocytes and triggering tumor cell death. Several other surface molecules, namely CD33, CD123, CD45, and CD70, can be targeted with monoclonal antibodies (mAbs) that exert different mechanisms of action and include naked and conjugated antibodies, bispecific T-cell engagers, trispecific killer engagers, and fusion proteins linked to toxins. These novel mAbs are currently under investigation for use as monotherapy or in combination with hypomethylating agents, BCL2 inhibitors, and chemotherapy in various clinical trials at different phases of development. Here, we review the main molecular targets and modes of action of novel mAb-based immunotherapies, which can represent the future of AML and higher risk MDS treatment.
Molecular Regulation of Androgen Receptors in Major Female Reproductive System Cancers
Authors: Wu Sujun , Yu Kun , Lian Zhengxing , Deng Shoulong
Published: July 2022

There are three main types of cancer in the female reproductive system, specifically ovarian cancer (OVCA), endometrial cancer (EC), and cervical cancer (CC). They are common malignant tumors in women worldwide, with high morbidity and mortality. In recent years, androgen receptors (ARs) have been found to be closely related to the occurrence, progression, prognosis, and drug resistance of these three types of tumors. This paper summarizes current views on the role of AR in female reproductive system cancer, the associations between female reproductive system cancers and AR expression and polymorphisms. AR regulates the downstream target genes transcriptional activity and the expression via interacting with coactivators/corepressors and upstream/downstream regulators and through the gene transcription mechanism of “classical A/AR signaling” or “non-classical AR signaling”, involving a large number of regulatory factors and signaling pathways. ARs take part in the processes of cancer cell proliferation, migration/invasion, cancer cell stemness, and chemotherapeutic drug resistance. These findings suggest that the AR and related regulators could target the treatment of female reproductive system cancer.
Large-Scale Multi-Omics Studies Provide New Insights into Blood Pressure Regulation
Authors: Kamali Zoha , Keaton Jacob M. , Haghjooy Javanmard Shaghayegh ,   International Consortium of Blood Pressure,   Million Veteran Program,   eQTLGen Consortium,   BIOS Consortium, Edwards Todd L. , Snieder Harold , Vaez Ahmad
Published: July 2022

Recent genome-wide association studies uncovered part of blood pressure’s heritability. However, there is still a vast gap between genetics and biology that needs to be bridged. Here, we followed up blood pressure genome-wide summary statistics of over 750,000 individuals, leveraging comprehensive epigenomic and transcriptomic data from blood with a follow-up in cardiovascular tissues to prioritise likely causal genes and underlying blood pressure mechanisms. We first prioritised genes based on coding consequences, multilayer molecular associations, blood pressure-associated expression levels, and coregulation evidence. Next, we followed up the prioritised genes in multilayer studies of genomics, epigenomics, and transcriptomics, functional enrichment, and their potential suitability as drug targets. Our analyses yielded 1880 likely causal genes for blood pressure, tens of which are targets of the available licensed drugs. We identified 34 novel genes for blood pressure, supported by more than one source of biological evidence. Twenty-eight (82%) of these new genes were successfully replicated by transcriptome-wide association analyses in a large independent cohort (n = ~220,000). We also found a substantial mediating role for epigenetic regulation of the prioritised genes. Our results provide new insights into genetic regulation of blood pressure in terms of likely causal genes and involved biological pathways offering opportunities for future translation into clinical practice.
Current Understanding of Immunological Skin Diseases: Atopic Dermatitis, Generalized Anhidrosis, and Drug Hypersensitivity
Authors: Hashizume Hideo
Published: July 2022

Recent dermatological research has progressed, particularly novel technologies and analytical methodologies, providing great advances in the exploration of previously poorly understood interactions between the skin—the outermost surface of humans—and the external environment [...]
Towards an Understanding of Retinal Diseases and Novel Treatment
Authors: Joachim Stephanie C.
Published: July 2022

The Special Issue, “Towards an Understanding of Retinal Diseases and Novel Treatment”, provides comprehensive information on retinal diseases such as glaucoma, age-related macular degeneration (AMD), diabetic retinopathy, retinitis pigmentosa (RP), and others [...]
The Qc5 Allele Increases Wheat Bread-Making Quality by Regulating SPA and SPR
Authors: Guo Zhenru , Chen Qing , Zhu Jing , Wang Yan , Li Yang , Li Qingcheng , Zhao Kan , Li Yue , Tang Rui , Shi Xiaoli , Tan Kenan , Kong Li , Jiang Yunfeng , Jiang Qiantao , Wang Jirui , Chen Guoyue , Wei Yuming , Zheng Youliang , Qi Pengfei
Published: July 2022

Common wheat (Triticum aestivum L.) is an important food crop with a unique processing quality. The Q gene positively regulates the processing quality of wheat, but the underlying mechanism remains unclear. Here, a new Q allele (Qc5) responsible for compact spikes and good bread performance was identified. Compared with the Q allele widely distributed in modern common wheat cultivars, Qc5 had a missense mutation outside the miRNA172-binding site. This missense mutation led to a more compact messenger RNA (mRNA) secondary structure around the miRNA172-binding region, resulting in increased Qc5 expression during the spike development stage and a consequent increase in spike density. Furthermore, this missense mutation weakened the physical interaction between Qc5 and storage protein activator (SPA) in seeds and suppressed the expression of storage protein repressor (SPR). These changes increased the grain protein content and improved the bread-making quality of wheat. In conclusion, a missense mutation increases Q expression because of the resulting highly folded mRNA secondary structure around the miRNA172-binding site. Furthermore, this mutation improves the bread-making quality of wheat by repressing the expression of SPR and influencing the physical interaction between Q and SPA. These findings provide new insights into the miRNA172-directed regulation of gene expression, with implications for wheat breeding.
KDM4C Contributes to Trophoblast-like Stem Cell Conversion from Porcine-Induced Pluripotent Stem Cells (piPSCs) via Regulating CDX2
Authors: Yu Shuai , Shen Qiaoyan , Zhang Rui , Wu Xiaolong , Zhang Juqing , Zhao Wenxu , Wu Xiaojie , Li Na , Peng Sha , Zhang Shiqiang , Yang Fan , Hua Jinlian
Published: July 2022

Studies on ESRRB-regulating porcine-induced pluripotent stem cells (piPSCs) converted to trophoblast-like stem cells (TLSCs) contribute to the understanding of early embryo development. However, the epigenetic modification regulation network during the conversion is poorly understood. Here, the global change in histone H3 Lysine 4, 9, 27, 36 methylation and Lysine 27 acetylation was investigated in piPSCs and TLSCs. We found a high modification profile of H3K36me2 in TLSCs compared to that of piPSCs, whereas the profiles of other modifications remained constant. KDM4C, a H3K36me3/2 demethylase, whose gene body region was combined with ESRRB, was upregulated in TLSCs. Moreover, KDM4 inhibitor supplementation rescued the AP-negative phenotype observed in TLSCs, confirming that KDM4C could regulate the pluripotency of TLSCs. Subsequently, KDM4C replenishment results show the significantly repressed proliferation and AP-positive staining of TLSCs. The expressions of CDX2 and KRT8 were also upregulated after KDM4C overexpression. In summary, these results show that KDM4C replaced the function of ESRRB. These findings reveal the unique and crucial role of KDM4C-mediated epigenetic chromatin modifications in determination of piPSCs’ fate and expand the understanding of the connection between piPSCs and TSCs.
Investigations on the Spin States of Two Mononuclear Iron(II) Complexes Based on N-Donor Tridentate Schiff Base Ligands Derived from Pyridine-2,6-Dicarboxaldehyde
Authors: Bayeh Yosef , Suryadevara Nithin , Schlittenhardt Sören , Gyepes Róbert , Sergawie Assefa , Hrobárik Peter , Linert Wolfgang , Ruben Mario , Thomas Madhu
Published: July 2022

Iron(II)-Schiff base complexes are a well-studied class of spin-crossover (SCO) active species due to their ability to interconvert between a paramagnetic high spin-state (HS, S = 2, 5T2) and a diamagnetic low spin-state (LS, S = 0, 1A1) by external stimuli under an appropriate ligand field. We have synthesized two mononuclear FeII complexes, viz., [Fe(L1)2](ClO4)2.CH3OH (1) and [Fe(L2)2](ClO4)2.2CH3CN (2), from two N6–coordinating tridentate Schiff bases derived from 2,6-bis[(benzylimino)methyl]pyridine. The complexes have been characterized by elemental analysis, electrospray ionization–mass spectrometry (ESI-MS), Fourier-transform infrared spectroscopy (FTIR), solution state nuclear magnetic resonance spectroscopy, 1H and 13C NMR (both theoretically and experimentally), single-crystal diffraction and magnetic susceptibility studies. The structural, spectroscopic and magnetic investigations revealed that 1 and 2 are with Fe–N6 distorted octahedral coordination geometry and remain locked in LS state throughout the measured temperature range from 5–350 K.
The Use of “Tail-Pedometers” to Evaluate the Impact of Dipterans in Feeder Cattle
Authors: Desquesnes Marc , Thaisungnoen Kornkanok , Chalermwong Piangjai , Nevot Adèle , Fossaert Clément , Lucas Antoine , Onju Sathaporn , Boonsaen Phoompong , Jittapalapong Sathaporn
Published: July 2022

Hematophagous flies are a pest for livestock; their direct impact reduces productivity, and they are vectors of parasites, bacteria and viruses. Their control using insecticides is inefficient and highly polluting. The validation of new control tools requires efficacy and cost-effectiveness evaluation. The quantification of hematophagous insects’ impact in livestock is a challenging prerequisite. Tail flicks counts can reliably evaluate fly-burden; however, visual records are tedious and time-consuming. In the present study, automation of tail flick counts was made through the use of pedometers attached to the tail, in two groups of feeder cattle. Group A was kept in a pen under the protection of a mosquito net, and Group B was kept in an open-air pen. The fly density of Group B was evaluated using fly traps. The apparent density per trap ranged from 130 to 1700 in the study. The mean pedometer records per 24 h ranged from 957+/−58 bits in Group A to 11,138+/−705 bits in Group B. The night/day records observed in Group A (200/800 bits) were drastically increased in Group B (1000–4000/4000–14,000 bits) and variable along seasons. A very high correlation was observed between fly density and visual records or pedometer records (PR). Two-hour PRs proved to be a reliable predictive tool for fly density. Moreover, the pedometers revealed an unsuspected but significant nuisance of mosquitoes, which should be thoroughly investigated.
Catalysts for Change: The Role of Nonprofits in Solving Single Ventricle Heart Disease
Authors: Davis Kaitlin A. , Pickles Diane M. , Keller Kirstie E.
Published: July 2022

Single ventricle (SV) heart disease comprises a spectrum of complex congenital heart defects (CHDs), including hypoplastic left heart syndrome (HLHS), one of the most common causes of death amongst infants with CHD. Despite its incompletely defined etiology and a dearth of curative solutions, SV is a solvable problem that can be addressed by unifying a nascent field that is ripe for investment, in part due to its high economic impact and growth potential. Here, we explore the landscape of SV and identify areas of opportunity that will yield an outsized impact through strategic investment that focuses on synchronization across disciplines, community involvement, and infrastructure development, and argue that nonprofits are the appropriate catalyst to spark transformative innovation and impact in the form of functional cures.
Topical Steroids and Glaucoma Filtration Surgery Outcomes: An In Vivo Confocal Study of the Conjunctiva
Authors: Mastropasqua Leonardo , Brescia Lorenza , D’Arcangelo Francesca , Nubile Mario , D’Onofrio Giada , Totta Michele , Perna Fabiana , Aloia Raffaella , Agnifili Luca
Published: July 2022

(1) Background: The purpose of this study is to investigate the effects of topical steroids on conjunctiva in patients undergoing filtration surgery (FS) for glaucoma by using confocal microscopy (CM); (2) Methods: One hundred and four glaucomatous patients were randomized to fluorometholone or lubricants four weeks before FS. CM was performed before treatments and pre-operatively. Dendritic and goblet cell densities (DCD, GCD), stromal meshwork reflectivity (SMR), vascular tortuosity (VT), and intra-ocular pressure (IOP) were the main outcomes. By evaluating treatments and outcomes (12-month success/failure) as categorical variables, patients were grouped into Group 1, 2, 3, or 4 (success/failure with fluorometholone, or lubricants); (3) Results: Twelve-month IOP was reduced in Groups 1 and 3 (p < 0.001). After treatments, DCD and SMR were reduced in Groups 1 and 2 (p < 0.01), and 1 and 3 (p < 0.05), respectively. Pre-operative DCD was lower in the steroid compared to lubricant group (p < 0.001), whereas SMR was lower in successful (1 and 3) compared to failed groups (2 and 4) (p = 0.004). There were no significant differences between the fluorometholone and lubricant groups for success percentages. The number of bleb management procedures and IOP lowering medications were lower in Group 1 compared to Groups 2–4 (p < 0.05); (4) Conclusions: Topical steroids mitigate conjunctival inflammation and lower the stromal density in patients undergoing FS. These modifications lead to less intensive post-operative management.
Symptomatic Bunionette Treated with Sliding Oblique Metatarsal Osteotomy—Case Series
Authors: Shen Yu-Ting , Huang Peng-Ju , Chen Shu-Jung , Chang Shun-Min
Published: July 2022

Background: The purpose of this study is to investigate the clinical and radiological results of a sliding oblique metatarsal osteotomy (SOMO) to correct bunionette deformity. Methods: We retrospectively reviewed 44 patients (51 feet, left/right: 29/22) from December 2010 to December 2018 who underwent SOMO and compared radiographic measurements and clinical outcome scores preoperatively and postoperatively. Radiographic measurements included 4th and 5th intermetatarsal angle (IMA), metatarsophalangeal angle (MTPA), and lateral deviation angle (LDA). Clinical outcome measurements included The American Orthopedic Foot and Ankle Society (AOFAS) score for lesser metatarsophalangeal procedures and visual analog scale (VAS) pain score. The mean follow-up period was 26.6 months (minimum 18 months). Based on Coughlin and Fallat classification, all cases were separated into four subtypes: 6 type I, 10 type II, 12 type III, 23 type IV cases included.) Results: All radiographic parameters significantly improved after SOMO procedure (IMA/MTPA/LDA, p value < 0.001). Clinical scores also showed a significant improvement in AOFAS and VAS scores (p value < 0.001). In terms of subgroup based on each type, both radiographic measurements and clinical scores revealed significant improvements in each subgroup (p value < 0.05), except LDA of type I subgroup (p value = 0.09). Three cases reported pin-tract infection but recovered with good healing after removal of the K-wire and a prescription of oral antibiotic. Conclusion: The SOMO procedure may be considered as a reliable and simple treatment for most types of bunionette deformity with satisfactory outcomes and no severe complications. Level of Evidence: Level IV, case series.
A Prospective Study of Etiology and Auditory Profiles in Infants with Congenital Unilateral Sensorineural Hearing Loss
Authors: Johansson Marlin , Karltorp Eva , Edholm Kaijsa , Drott Maria , Berninger Erik
Published: July 2022

Congenital unilateral sensorineural hearing loss (uSNHL) is associated with speech-language delays and academic difficulties. Yet, controversy exists in the choice of diagnosis and intervention methods. A cross-sectional prospective design was used to study hearing loss cause in twenty infants with congenital uSNHL consecutively recruited from a universal neonatal hearing-screening program. All normal-hearing ears showed ≤20 dB nHL auditory brainstem response (ABR) thresholds (ABRthrs). The impaired ear median ABRthr was 55 dB nHL, where 40% had no recordable ABRthr. None of the subjects tested positive for congenital cytomegalovirus (CMV) infection. Fourteen subjects agreed to participate in magnetic resonance imaging (MRI). Malformations were common for all degrees of uSNHL and found in 64% of all scans. Half of the MRIs demonstrated cochlear nerve aplasia or severe hypoplasia and 29% showed inner ear malformations. Impaired ear and normal-hearing ear ABR input/output functions on a group level for subjects with ABRthrs < 90 dB nHL were parallel shifted. A significant difference in interaural acoustic reflex thresholds (ARTs) existed. In congenital uSNHL, MRI is powerful in finding a possible hearing loss cause, while congenital CMV infection may be relatively uncommon. ABRs and ARTs indicated an absence of loudness recruitment, with implications for further research on hearing devices.
Self-Reported Mental and Physical Measures in Adult Fontan Patients
Authors: Schamroth Pravda Nili , Zusman Oren , Richter Ilan , Blieden Leonard , Vig Shahar , Marchushamer Ilan , Dadashev Alexander , Razon Yaron , Kornowski Ran , Hirsch Rafael
Published: July 2022

Introduction: The Fontan procedure is a palliative operation for patients with single functional ventricles, arising from a heterogeneous group of heart defects. There is a considerable gap in evidence regarding the self-reported physical and mental health of these patients surviving to adulthood. Methods and Results: We administered the PROMIS® Global Short Form (v 1.2) to Fontan patients during their scheduled clinic visits during 2017–2018. The raw PROMIS scores were subsequently converted to standardized T-scores, where the mean performance was 50 for the general population. We used Cronbach’s alpha to assess reliability, with >0.8 considered good. A total of 42 patients were included. The median age was 30 (IQR: 24–34) years and 59% (95% CI: 43–74%) were female. The median time from birth to operation was 4.5 (IQR: 3–8) years, with 55% having an extracardiac Fontan. The questionnaire had good internal reliability with an alpha of 0.87. Seventy-one percent of respondents rated their overall health as “excellent” or “good”. The mean T-score for physical health was 46.6, lower than the age-group mean (51.6, p < 0.001). The mean T-score for mental health was 53.3, higher than the age-group mean (48.5, p < 0.001). T-scores showed strong correlation with each other (r = 0.7) and weak correlation with age and time from procedure. There was no association of T-score with diagnosis or operation type. Conclusions: Adult Fontan patients report better mental health despite worse reporting physical health compared with the age group means. Patient-reported measures can provide clinically meaningful insights about the care of patients with complex congenital heart disease.
Risks in Induction of Platelet Aggregation and Enhanced Blood Clot Formation in Platelet Lysate Therapy: A Pilot Study
Authors: Wen Ying-Hao , Lee Chen-Fang , Chen Yu-Ju , Chang Gwo-Jyh , Chong Kowit-Yu
Published: July 2022

Platelet concentrates (PCs) are widely used in regenerative medicine; as it is produced from freeze–thawing PC, platelet lysate (PL) has a longer shelf life. The thrombotic risk of PL therapy needs to be explored since PL and PC contain cytokines that contribute to platelet aggregation and thrombus formation. Whole blood samples of 20 healthy subjects were collected; PL was produced from PCs with expired shelf life through freeze–thawing. The direct mixing of PL with platelet-rich plasma (PRP) or whole blood was performed. In addition, rotational thromboelastometry (ROTEM) was used to investigate whether PL enhanced coagulation in vitro; the effects of fibrinogen depletion and anticoagulants were evaluated to prevent hypercoagulation. The results showed that PL induced platelet aggregation in both PRP and whole blood. In ROTEM assays, PL was shown to cause a significantly lower clotting onset time (COT) and clot formation time (CFT), and a significantly greater α angle and maximum clot firmness (MCF). Compared with the controls, which were 1:1 mixtures of normal saline and whole blood, fibrinogen depletion of PL showed no significant difference in CFT, α angle and MCF. Moreover, heparin- and rivaroxaban-added PL groups demonstrated no clot formation in ROTEM assays. Platelet lysate-induced hypercoagulability was demonstrated in vitro in the present study, which could be prevented by fibrinogen depletion or the addition of an anticoagulant.
Efficacy and Safety of Two Salts of Trientine in the Treatment of Wilson’s Disease
Authors: Woimant France , Debray Dominique , Morvan Erwan , Obadia Mickael Alexandre , Poujois Aurélia
Published: July 2022

Background: Wilson’s disease (WD) is one of the few genetic disorders that can be successfully treated with pharmacological agents. Copper-chelating agents (D-penicillamine and Trientine salts) and zinc salts have been demonstrated to be effective. There are two salts of trientine. Trientine dihydrochloride salt (TETA 2HCL) is unstable at room temperature and requires storage at 2–8 °C. Trientine tetrahydrochloride (TETA 4HCL) is a more stable salt of trientine that can be stored at room temperature. No comparative study between both of the salts of trientine has been performed to date. As the two chemical forms were available in France between 1970 and 2009, we conducted a study to evaluate their efficacy and safety profiles. Methods: This retrospective cohort study was conducted by reviewing data from the national WD registry in France. Forty-three WD patients who received TETA 2HCL or TETA 4HCL monotherapy for at least one year until 2010 were included. The primary endpoints were hepatic and neurological outcomes. Secondary endpoints were the events leading to a discontinuation of medication. Results: Changes in medication were common, leading to the analysis of 57 treatment sequences of TETA 4HCL or TETA 2HCL. The mean duration of treatment sequence was significantly longer in the TETA 4 HCL group (12.6 years) than in the TETA 2HCL group (7.6 years) (p = 0.011). Ten patients experienced both trientine salts: eight stopped TETA 4 HCL (six had a hepatologic phenotype and two had a neurological phenotype) because this treatment was not available anymore (mean duration 7.4 years). Three of these patients already experienced TETA 2 HCL before the sequence. Two patients with a hepatologic phenotype (one had a previous sequence of TETA 4 HCL before) stopped TETA 2 HCL because of cold storage issues (mean duration 42.8 years). The total number of sequences was 57. All of the patients were clinically stable. No difference in efficacy was detected. Both treatments were well tolerated, except for a case of recurrence of lupus erythematosus-like syndrome in the TETA 2HCL group. The major reason for interruption of TETA 4HCL was due to a discontinuation in production of this salt. The reasons for stopping TETA 2HCL were mainly due to adherence issues largely attributed to the cold storage requirement. Conclusions: The two salts of trientine were effective in treating patients with WD. However, interruption of TETA 2HCL was frequent, linked to the cold storage requirement. As adherence to treatment is a key factor in the successful management of WD, physicians need to be even more vigilant in detecting adherence difficulties in patients receiving treatment with TETA 2HCL.
Clinical Islet Transplantation Covered by Health Insurance in Japan
Authors: Noguchi Hirofumi
Published: July 2022

Pancreatic islet transplantation is a treatment option for patients with type 1 diabetes mellitus and has been performed in various countries [...]
Exploring the High Frequencies AC Conductivity Response in Disordered Materials by Using the Damped Harmonic Oscillator
Authors: Tsonos Christos
Published: July 2022

The AC conductivity response of disordered materials follows a universal power law of the form σ′(ω)∝ωn at the low frequency regime, with the power exponent values in the range 0 < n < 1. At the high frequency regime, in many experimental data of different disordered materials, superlinear values of the power exponent n were observed. The observed superlinear values of the power exponent are usually within 1<n<2, but in some cases values n>2 were detected. The present work is based on the definitions of electromagnetic theory as well as the Havriliak–Negami equation and the damped harmonic oscillator equation, which are widely used for the description of dielectric relaxation mechanisms and vibration modes in the THz frequency region, respectively. This work focuses mainly on investigating the parameters that affect the power exponent and the range of possible n values.
Color Stability Determination of CAD/CAM Milled and 3D Printed Acrylic Resins for Denture Bases: A Narrative Review
Authors: Dimitrova Mariya , Corsalini Massimo , Kazakova Rada , Vlahova Angelina , Barile Giuseppe , Dell’Olio Fabio , Tomova Zlatina , Kazakov Stoyan , Capodiferro Saverio
Published: July 2022

The aim of this paper is to review the available literature on the different methods for color stability determination of CAD/CAM milled and 3D printed resins for denture bases. The methodology included applying a search strategy, defining inclusion and exclusion criteria and selecting studies to summarize the results. Searches of PubMed, Scopus, and Embase databases were performed independently by three reviewers to gather the literature published between 1998 and 2022. A total of 186 titles were obtained from the electronic database, and the application of exclusion criteria resulted in the identification of 66 articles pertaining to the different methods for color stability determination of CAD/CAM acrylic resins for denture bases. Color change in dental materials is clinically very important for the dental operator, as it determines the clinical serviceability of the material. Discoloration of the denture bases can be evaluated with various instruments and methods. Dental resins may undergo color changes over time due to intrinsic and/or extrinsic factors. The extrinsic factors are considered the more frequent causes of color changes. According to a number of studies, CAD/CAM fabricated acrylics have achieved better color stability than the conventional PMMA (polymethyl methacrylate) resins.
Failures and Flaws in Fused Deposition Modeling (FDM) Additively Manufactured Polymers and Composites
Authors: Baechle-Clayton Maggie , Loos Elizabeth , Taheri Mohammad , Taheri Hossein
Published: July 2022

In this review, the potential failures and flaws associated with fused deposition modeling (FDM) or fused filament fabrication (FFF) 3D printing technology are highlighted. The focus of this article is on presenting the failures and flaws that are caused by the operational standpoints and which are based on the many years of experience with current and emerging materials and equipment for the 3D printing of polymers and composites using the FDM/FFF method. FDM or FFF 3D printing, which is also known as an additive manufacturing (AM) technique, is a material processing and fabrication method where the raw material, usually in the form of filaments, is added layer-by-layer to create a three-dimensional part from a computer designed model. As expected, there are many advantages in terms of material usage, fabrication time, the complexity of the part, and the ease of use in FDM/FFF, which are extensively discussed in many articles. However, to upgrade the application of this technology from public general usage and prototyping to large-scale production use, as well as to be certain about the integrity of the parts even in a prototype, the quality and structural properties of the products become a big concern. This study provides discussions and insights into the potential factors that can cause the failure of 3D printers when producing a part and presents the type and characteristics of potential flaws that can happen in the produced parts. Common defects posed by FDM printing have been discussed, and common nondestructive detection methods to identify these flaws both in-process and after the process is completed are discussed. The discussions on the failures and flaws in machines provides useful information on troubleshooting the process if they happen, and the review on the failures and flaws in parts helps researchers and operators learn about the causes and effects of the flaws in a practical way.