bluuebunny/crossref_metadata_2025 huggingface dataset:
“Chamber #8” – a holistic approach of high-throughput non-destructive assessment of plant roots
Authors: Claussen Joelle , Wittenberg Thomas , Uhlmann Norman , Gerth Stefan
Published: January 2024

Introduction

In the past years, it has been observed that the breeding of plants has become more challenging, as the visible difference in phenotypic data is much smaller than decades ago. With the ongoing climate change, it is necessary to breed crops that can cope with shifting climatic conditions. To select good breeding candidates for the future, phenotypic experiments can be conducted under climate-controlled conditions. Above-ground traits can be assessed with different optical sensors, but for the root growth, access to non-destructively measured traits is much more challenging. Even though MRI or CT imaging techniques have been established in the past years, they rely on an adequate infrastructure for the automatic handling of the pots as well as the controlled climate.

Methods

To address both challenges simultaneously, the non-destructive imaging of plant roots combined with a highly automated and standardized mid-throughput approach, we developed a workflow and an integrated scanning facility to study root growth. Our “chamber #8” contains a climate chamber, a material flow control, an irrigation system, an X-ray system, a database for automatic data collection, and post-processing. The goals of this approach are to reduce the human interaction with the various components of the facility to a minimum on one hand, and to automate and standardize the complete process from plant care via measurements to root trait calculation on the other. The user receives standardized phenotypic traits and properties that were collected objectively.

Results

The proposed holistic approach allows us to study root growth of plants in a field-like substrate non-destructively over a defined period and to calculate phenotypic traits of root architecture. For different crops, genotypic differences can be observed in response to climatic conditions which have already been applied to a wide variety of root structures, such as potatoes, cassava, or corn.

Discussion

It enables breeders and scientists non-destructive access to root traits. Additionally, due to the non-destructive nature of X-ray computed tomography, the analysis of time series for root growing experiments is possible and enables the observation of kinetic traits. Furthermore, using this automation scheme for simultaneously controlled plant breeding and non-destructive testing reduces the involvement of human resources.
Performance of plant-produced RBDs as SARS-CoV-2 diagnostic reagents: a tale of two plant platforms
Authors: Santoni Mattia , Gutierrez-Valdes Noemi , Pivotto Denise , Zanichelli Elena , Rosa Anthony , Sobrino-Mengual Guillermo , Balieu Juliette , Lerouge Patrice , Bardor Muriel , Cecchetto Riccardo , Compri Monica , Mazzariol Annarita , Ritala Anneli , Avesani Linda
Published: January 2024

The COVID-19 pandemic has underscored the need for rapid and cost-effective diagnostic tools. Serological tests, particularly those measuring antibodies targeting the receptor-binding domain (RBD) of the virus, play a pivotal role in tracking infection dynamics and vaccine effectiveness. In this study, we aimed to develop a simple enzyme-linked immunosorbent assay (ELISA) for measuring RBD-specific antibodies, comparing two plant-based platforms for diagnostic reagent production. We chose to retain RBD in the endoplasmic reticulum (ER) to prevent potential immunoreactivity issues associated with plant-specific glycans. We produced ER-retained RBD in two plant systems: a stable transformation of BY-2 plant cell culture (BY2-RBD) and a transient transformation in Nicotiana benthamiana using the MagnICON system (NB-RBD). Both systems demonstrated their suitability, with varying yields and production timelines. The plant-made proteins revealed unexpected differences in N-glycan profiles, with BY2-RBD displaying oligo-mannosidic N-glycans and NB-RBD exhibiting a more complex glycan profile. This difference may be attributed to higher recombinant protein synthesis in the N. benthamiana system, potentially overloading the ER retention signal, causing some proteins to traffic to the Golgi apparatus. When used as diagnostic reagents in ELISA, BY2-RBD outperformed NB-RBD in terms of sensitivity, specificity, and correlation with a commercial kit. This discrepancy may be due to the distinct glycan profiles, as complex glycans on NB-RBD may impact immunoreactivity. In conclusion, our study highlights the potential of plant-based systems for rapid diagnostic reagent production during emergencies. However, transient expression systems, while offering shorter timelines, introduce higher heterogeneity in recombinant protein forms, necessitating careful consideration in serological test development.
Impact of physical activity on executive functions: a moderated mediation model
Authors: Zhao Guoguo , Sun Kaihong , Fu Jian , Li Zhe , Liu Dongbin , Tian Xin , Yang Jiehui , Zhang Qiushi
Published: January 2024

Objective

To provide both empirical support and a theoretical framework for systematically improving and optimizing the cognitive capabilities of college students through physical activity, while considering the mediating and regulating impacts of self-efficacy and negative emotion.

Methods

The study employed an overall random sampling method, examining 500 college students from five universities in Jiangsu Province using the Physical Activity Rating Scale (PARS-3), Adult Executive Function Scale (ADEXI), Positive and Negative Emotion Scale (PANAS), and General Self-Efficacy Scale (GSES).

Results

The findings indicated that the average age of the participants was 18.41 ± 0.73 years, encompassing 215 male students (43%), and 185 female students (57%). Engagement in physical activity was significantly and positively correlated with executive function (β = 0.246, p < 0.01), inversely associated with negative emotion (β = −0.137, p < 0.01), and demonstrated a significant positive predictive impact on self-efficacy (β = 0.183, p < 0.01). Self-efficacy was observed to partially mediate the relationship between executive function and physical activity. In addition, negative mood was identified as playing a partial mediating and modifying role in the relationship between executive function and physical activity.

Conclusion

Increasing college students’ daily physical activity participation not only benefits their executive function, self-efficacy, and confidence levels but also exerts a limited positive impact on negative mood, with the potential to regulate the intensity of negative emotion.
Taxonomic structure in a set of abstract concepts
Authors: Persichetti Andrew S. , Shao Jiayu , Denning Joseph M. , Gotts Stephen J. , Martin Alex
Published: January 2024

A large portion of human knowledge comprises “abstract” concepts that lack readily perceivable properties (e.g., “love” and “justice”). Since abstract concepts lack such properties, they have historically been treated as an undifferentiated category of knowledge in the psychology and neuropsychology literatures. More recently, the categorical structure of abstract concepts is often explored using paradigms that ask participants to make explicit judgments about a set of concepts along dimensions that are predetermined by the experimenter. Such methods require the experimenter to select dimensions that are relevant to the concepts and further that people make explicit judgments that accurately reflect their mental representations. We bypassed these requirements by collecting two large sets of non-verbal and implicit judgments about which dimensions are relevant to the similarity between pairs of 50 abstract nouns to determine the representational space of the concepts. We then identified categories within the representational space using a clustering procedure that required categories to replicate across two independent data sets. In a separate experiment, we used automatic semantic priming to further validate the categories and to show that they are an improvement over categories that were defined within the same set of abstract concepts using explicit ratings along predetermined dimensions. These results demonstrate that abstract concepts can be characterized beyond their negative relation to concrete concepts and that categories of abstract concepts can be defined without using a priori dimensions for the concepts or explicit judgments from participants.
The role of interpersonal coordination dynamics in alliance rupture and repair processes in psychotherapy—A systematic review
Authors: Høgenhaug S. S. , Kongerslev M. T. , Kjaersdam Telléus G.
Published: January 2024

Introduction

The purpose of this systematic review is to expand our knowledge of the underlying mechanisms of the alliance in psychotherapy. This is done by examining the association between alliance rupture and repair processes and interpersonal coordination dynamics.

Method

A systematic review based on PRISMA guidelines was conducted, aimed at papers investigating the association between alliance rupture and repair episodes and different behavioral modalities (i.e., physiology, movement) in the psychotherapeutic interaction. Seventeen studies were included for full text-analysis.

Results

The results indicate that rupture and repair episodes were associated with interpersonal coordination dynamics. Different modalities (movement, heart rate, and vocalization) were found to serve as markers for alliance rupture and repair events. Facial expressions, physiological arousal, vocalization, and behavior were found to play important roles in the therapeutic interaction in relation to mutual emotion regulation, empathic response, safety, trust, and meaning-making.

Discussion

Limitations of this review are discussed, including the great methodological variation and selection bias observed in the reviewed studies. Recommendations for future research in this area are presented. Overall, interpersonal coordination dynamics was found to have the potential to help identify and manage alliance ruptures and foster repairs in the therapeutic interaction, which has high potential for future clinical work and training.
Values and physical activity among sports science students in France and China: a transcultural analysis
Authors: Liang Yan , Rascle Olivier , Hanel Paul H. P. , Yang Jian , Souchon Nicolas
Published: January 2024

Objective

The aim of this study was to analyze the relationships between values and physical activity in France (a Western European individualistic country) and in China (an East Asian collectivist country).

Method

Six hundred and twenty-seven sport science students in France (N = 308, Mage = 18.99, SD = 1.64) and China (N = 319, Mage = 20.44, SD = 1.09) completed the International Physical Activity Questionnaire long version and the Portrait Values Questionnaire.

Results

In both France and China, moderated regression analysis revealed that hedonism positively/negatively predicted physical activity, while security-societal, security-personal, and conformity-rules values negatively predicted physical activity. In contrast, stimulation and universalism-nature values positively predicted physical activity only in France. In China, benevolence and benevolence-care positively predicted physical activity, while power dominance negatively predicted physical activity. Additionally, we found evidence of measurement invariance of the value questionnaire.

Discussion and conclusion

Our findings add to the literature by showing that the value–behavior link is partly the same across countries and partly different. Further, our findings show that for certain populations, the previously established hierarchy of human values does not replicate.
Barriers and enablers of integrated care in the UK: a rapid evidence review of review articles and grey literature 2018–2022
Authors: Thomson Linda J. M. , Chatterjee Helen J.
Published: January 2024

Integrated care refers to person-centered and coordinated, health and social care, and community services. Integrated care systems are partnerships of organizations that deliver health and care services which were placed on a statutory footing in England, April 2022. Due to the need for fast, accessible, and relevant evidence, a rapid review was conducted according to World Health Organization methods to determine barriers and enablers of integrated care across the United Kingdom, 2018–2022. Nine databases were searched for review articles reporting evaluation of integrated care interventions involving medical (clinical and diagnostic) and nonmedical (public health services and community-based or social care/person-centred care) approaches, quality checked with the Critical Appraisal Skills Program qualitative checklist. OpenGrey and hand searches were used to identify grey literature, quality checked with the Authority, Accuracy, Coverage, Objectivity, Date, and Significance checklist. Thirty-four reviews and 21 grey literature reports fitted inclusion criteria of adult physical/mental health outcomes/multiple morbidities. Thematic analysis revealed six themes (collaborative approach; costs; evidence and evaluation; integration of care; professional roles; service user factors) with 20 subthemes including key barriers (cost effectiveness; effectiveness of integrated care; evaluation methods; focus of evidence; future research; impact of integration) and enablers (accessing care; collaboration and partnership; concept of integration; inter-professional relationships; person-centered ethos). Findings indicated a paucity of robust research to evaluate such interventions and lack of standardized methodology to assess cost effectiveness, although there is growing interest in co-production that has engendered information sharing and reduced duplication, and inter-professional collaborations that have bridged task-related gaps and overlaps. The importance of identifying elements of integrated care associated with successful outcomes and determining sustainability of interventions meeting joined-up care and preventive population health objectives was highlighted.
Regional variation in NAFLD prevalence and risk factors among people living with HIV in Europe: a meta-analysis
Authors: Jin Dachuan , Jin Shunqin , Zhou Tao , Cui Zhongfeng , Guo Baoqiang , Li Guangming , Zhang Chunming
Published: January 2024

Background and Aim

Europe faces an elevated risk of nonalcoholic fatty liver disease (NAFLD) among people living with HIV (PLWH), contributing to the region’s highest global burden of NAFLD. However, the prevalence of NAFLD across various European countries and regions remains unclear. This study aims to investigate the prevalence and risk factors associated with NAFLD among PLWH across European countries.

Methods

A systematic search was conducted across four databases: PubMed, Embase, Web of Science, and Cochrane Library. Data on the prevalence of NAFLD, nonalcoholic steatohepatitis (NASH), and fibrosis, as well as the associated risk factors, were collected among PLWH in Europe.

Results

Thirty-six studies from 13 European nations were included. The prevalence of NAFLD, NASH, and fibrosis were 42% (95%CI 37–48), 35% (95%CI 21–50) and 13% (95%CI 10–15), respectively. Male gender, BMI, waist circumference, Diabetes, hypertension, metabolic syndrome, dyslipidemia, triglycerides, HDL, LDL, ALT, AST, and years on antiretroviral therapy (ART) were found to be risk factors for NAFLD. High BMI and triglycerides were associated with NASH. Patients with high BMI and triglycerides are at increased risk of significant liver fibrosis.

Conclusion

The high prevalence of NAFLD, NASH, and fibrosis among PLWH in Europe highlights the need for early screening, intervention, and increased research focus on adolescents living with HIV. Furthermore, the significant variations observed between countries and regions underscore the influence of related risk factors.
Impact of floor covering on wheelchair rugby players: analysis of rolling performance
Authors: Vigié O. , Faupin A. , Ngo M-A. , Fauvet C. , Pradon D.
Published: January 2024

Introduction

Despite the increased interest in indoor wheelchair sports in many countries, research on the effect of floor coverings on sports performance is limited. Currently, there are no specific guidelines for covering characteristics for wheelchair sports, whether for competitive or recreational purposes. This study aimed to determine the impact of floor coverings on the biomechanical parameters of manual wheelchair propulsion for wheelchair rugby practice.

Methods

Ten wheelchair rugby players performed 6 maximum-velocity sprints over 20 meters, with a 20-second recovery time between sprints, on 3 different coverings, using their personal sports wheelchairs. The coverings were: wood parquet, Gerflor TX System Endurance®, and a plastic synthetic covering (balatum). Performance and propulsion technique variables were collected using inertial measurement units (265 Hz, Kinvent, France). Additionally, rolling resistance quantification tests were conducted on each covering.

Results

Rolling resistance was lowest on the wood parquet, with an average value of 3.98 ± 0.97 N. Best sprint performance was achieved on the wood parquet. The fatigue index on the parquet was significantly lower than on the balatum (p < 0.05).

Discussion

Our results highlight that floor surface influences both performance and propulsion technique variables. Therefore, we recommend performing wheelchair rugby training on wood parquet to optimize performance. It is also important to consider the impact of different coverings on sprint performance when organizing player rotations to maintain a high level of competition during tournaments.
Exploring farmers’ communication pattern and satisfaction regarding the adoption of Agromet advisory services in semi-arid regions of southern India
Authors: Rohit Jagriti , Bal S. K. , Anshida Beevi C. N. , Nagasree K. , Ravi Shankar K. , Nirmala G. , Pushpanjali  , Samuel Josily , Veni V. Girija , Nagarjuna Kumar R. , Pankaj Prabhat Kumar , Prabhakar Mathyam , Singh Vinod Kumar
Published: January 2024

Agriculture is significantly impacted by the variability in weather patterns, imposing substantial constraints on farmers’ ability to make informed tactical and strategic decisions regarding their crops. Seasonal climate projections have shown potential for informing agricultural decisions, but the actual adoption of climate information by farmers has been relatively slow and limited. The present study was conducted with the objective of investigating the characteristics of adopters, the communication network, and the level of farmer’s satisfaction concerning the adoption and continued use of Agromet Advisories Services (AAS). Two semi-arid districts, namely Kurnool and Anantapur in Andhra Pradesh, were purposively selected, wherein 280 farmers constituted the sampling frame. In this study, social network analysis (SNA) was conducted to examine the peer-to-peer communication patterns, while importance-performance analysis (IPA) was employed to evaluate farmer’s satisfaction, contributing to the continued adoption of AAS. The findings revealed that with regard to personality and communication characteristics, farmers were in the low category for their ability to cope with uncertainties and risk and even for their information-seeking behavior. Furthermore, the results showed farmers to be highly satisfied with the overall adoption of AAS. However, the IPA matrix revealed that among the nine attributes, the credibility of the forecasts needed refinement to promote sustained adoption. Excessive emphasis was placed on attributes such as the frequency of forecasts, which could be channeled into other initiatives. Peer-to-peer communication emerged as a crucial strategy in the adoption of AAS. Governments, non-governmental organizations, and extension functionaries should make a concerted effort to enhance the continued adoption of AAS by involving local stakeholders in sharing and participating in climate information production, forming farmer’s groups, and focusing on farmers’ literacy toward AAS.
Shrimp industry in China: overview of the trends in the production, imports and exports during the last two decades, challenges, and outlook
Authors: N’Souvi Kodjo , Sun Chen , Che Bin , Vodounon Anani
Published: January 2024

China’s shrimp farming industry has experienced significant growth in recent years. With such a development, some major constraints linked to the industry’s sustainable development have emerged. This paper aims to present an overview of China’s shrimp industry over the last two decades, with special attention to farm-raised shrimp both in marine and inland waters. Documentary research on its recent evolution was carried out based on data collected from China Fishery Statistics Yearbooks 2003 to 2022 coupled with data from FAO. Findings indicated that there has been an upward trend in China’s total shrimp production over the last 20 years, with a growth rate of 120%, representing an average annual growth rate of 4.3%. The observed higher shrimp production was driven by expanded shrimp aquaculture production, with major species dominated by the Pacific whiteleg shrimp (litopenaeus vannamei), the black tiger shrimp (Penaeus monodon), the Chinese shrimp (Penaeus chinensis), and the Japanese prawn (Penaeus japonicus). Challenges associated with the intensification of the production and the driven environmental deterioration need to be properly addressed. First, the shrimp farming industry should be practiced while considering both environment and ecosystem protection. Applying such an organic farming principle presupposes some practices for addressing the existing issues threatening the sustainable growth of the industry. To that end, there is a need for innovative techniques that should be strengthened. Second, possible solutions to shrimp disease problems, namely prevention, early diagnosis, and various control techniques, should also be developed and improved, with an emphasis on the former. In addition, encouraging the use of good-quality feed in appropriate quantity and form is also of paramount importance. It is thus worth noting that further policies need to focus on promoting a large range of ecological shrimp aquaculture technologies that should be encouraged among farmers.
Thoracic electrical impedance tomography identifies heterogeneity in lungs associated with respiratory disease in cattle. A pilot study
Authors: Brabant Olivia , Karpievitch Yuliya V. , Gwatimba Alphons , Ditcham William , Ho Ho Yin , Raisis Anthea , Mosing Martina
Published: January 2024

Respiratory disease in cattle is a significant global concern, yet current diagnostic methods are limited, and there is a lack of crush-side tests for detecting active disease. To address this gap, we propose utilizing electrical impedance tomography (EIT), a non-invasive imaging technique that provides real-time visualization of lung ventilation dynamics. The study included adult cattle from farms in Western Australia. The cattle were restrained in a crush. A standardized respiratory scoring system, which combined visual, auscultation, and clinical scores, was conducted by two non-conferring clinicians for each animal. The scores were blinded and averaged. During assessment, an EIT electrode belt was placed around the thorax. EIT recordings of ten suitable breaths were taken for analysis before the cattle were released back to the herd. Based on the combined examination scoring, the cattle were categorized as having healthy or diseased lungs. To allow visual interpretation of each breath and enable the creation of the quartile ventilation ratio (VQR), Flow/Tidal Impedance Variation curves (F/TIV) were generated for each breath. The analysis focused on two EIT variables: The novel VQR over time during inhalation and exhalation and global expiratory impedance (TIVEXP) adjusted by breath length. A mixed effects model was used to compare these variables between healthy and diseased cattle. Ten adult cattle of mixed ages were used in the current analysis. Five cattle were scored as healthy and five as diseased. There was a significant difference in the examination scores between the healthy and diseased group (P = 0.03). A significant difference in VQR during inhalation (P = 0.03) was observed between the healthy and diseased groups. No difference was seen in VQR over time during exhalation (P = 0.3). The TIVEXP was not different between groups (P = 0.36). In this study, EIT was able to detect differences in inhalation mechanics when comparing healthy and diseased cattle as defined via clinical examination, highlighting the clinical utility of EIT.
Evaluation of the protective efficacy of six major immunogenic proteins of Mycoplasma Synoviae
Authors: Han Shuizhong , Wang Ying , Chang Wenchi , Wang Lizhen , Fang Junyang , Han Jingjing , Hou Xiaolan , Qi Xuefeng , Wang Jingyu
Published: January 2024

Mycoplasma synoviae (MS) is a primary avian pathogen prevalent worldwide that causes airsacculitis and synovitis in birds. Vaccination is recommended as the most cost-effective strategy in the control of MS infection. Novel alternative vaccines are needed for eradicating and controlling MS infection in flocks. DnaK, enolase, elongation factor Tu (EF-Tu), MSPB, NADH oxidase and LP78 are the major immunogenic antigens of MS and are promising targets for subunit vaccine candidates. In the present study, genes encoding DnaK, enolase, EF-Tu, MSPB, LP78, and NADH oxidase were cloned and expressed in Escherichia coli. Enzyme-linked immunosorbent assay showed that the six recombinant proteins were recognized by convalescent sera, indicating that they were expressed during infection. Two injections of the six subunit vaccines induced a robust antibody response and increased the concentrations of IFN-γ and IL-4, especially rEnolase and rEF-Tu. The proliferation of peripheral blood lymphocytes was enhanced in all of the immunized groups. Chickens immunized with rEnolase, rEF-Tu, rLP78, and rMSPB conferred significant protection against MS infection, as indicated by significantly lower DNA copies in the trachea, lower scores of air sac lesions, and lesser tracheal mucosal thickness than that in the challenge control. Especially, rEnolase provided the best protective efficacy, followed by rEF-Tu, rMSPB, and rLP78. Our finds demonstrate that the subunit vaccines and bacterin can only reduce the lesions caused by MS infection, but not prevent colonization of the organism. Our findings may contribute to the development of novel vaccine agents against MS infection.
Supratentorial meningeal melanocytoma mimicking meningioma: case report and literature review
Authors: Ferreira de Araújo Mayle Gomes , Almondes Santana Lemos Luiz Euripedes , Negromonte Guerra Pedro Lucas , Marcia dos Santos Lima Didjurgeit Fernanda , Batista Cezar Auricelio , Faquini Igor Vilela , Cirne de Azevedo Filho Hildo Rocha
Published: January 2024

Introduction: Primary melanocytic tumors originating from CNS melanocytes are rare, with a low incidence of 0.7 cases per 10 million annually. This study focuses on primary leptomeningeal melanocytomas, emphasizing their epidemiology, clinical characteristics, and diagnostic challenges. Despite their infrequency, these tumors warrant attention due to their unique features and potential for local recurrence.

Case Report: A 32-year-old female presented with syncope and seizures, leading to the discovery of two left-sided supratentorial lesions initially misidentified as convexity meningiomas. Detailed imaging suggested meningioma-like features, but intraoperative findings revealed unexpected hyperpigmented lesions. Histopathological examination, supported by immunohistochemistry, confirmed primary leptomeningeal melanocytoma. The surgical approach and subsequent management are discussed.

Discussion: The discussion emphasizes challenges in diagnosing primary leptomeningeal melanocytomas. Treatment debates, especially regarding adjuvant radiotherapy, are explored. Recurrence risks stress the importance of vigilant follow-up, advocating for complete surgical resection as the primary approach. The rarity of supratentorial cases adds complexity to diagnosis, necessitating a multidisciplinary approach. Insights from this case contribute to understanding and managing primary leptomeningeal melanocytomas, addressing challenges in differentiation from more common tumors and prompting ongoing research for refined diagnostics and optimized treatments.

Conclusion: This study contributes insights into primary leptomeningeal melanocytomas, highlighting their rarity in supratentorial regions. The case underscores the importance of a multidisciplinary approach, incorporating clinical, radiological, and histopathological expertise for accurate diagnosis and tailored management. Ongoing research is crucial to refine treatment strategies, enhance prognostic precision, and improve outcomes for individuals with this uncommon CNS neoplasm.
KRASG12C mutant lung adenocarcinoma: unique biology, novel therapies and new challenges
Authors: Moldvay Judit , Tímár József
Published: January 2024

KRAS mutant lung cancer is the most prevalent molecular subclass of adenocarcinoma (LUAD), which is a heterogenous group depending on the mutation-type which affects not only the function of the oncogene but affects the biological behavior of the cancer as well. Furthermore, KRAS mutation affects radiation sensitivity but leads also to bevacizumab and bisphosphonate resistance as well. It was highly significant that allele specific irreversible inhibitors have been developed for the smoking associated G12C mutant KRAS (sotorasib and adagrasib). Based on trial data both sotorasib and adagrasib obtained conditional approval by FDA for the treatment of previously treated advanced LUAD. Similar to other target therapies, clinical administration of KRASG12C inhibitors (sotorasib and adagrasib) resulted in acquired resistance due to various genetic changes not only in KRAS but in other oncogenes as well. Recent clinical studies are aiming to increase the efficacy of G12C inhibitors by novel combination strategies.
Topology Optimization of a Single-Point Diamond-Turning Fixture for a Deployable Primary Mirror Telescope
Authors: Bourgenot Cyril , Krumins Valdis , Bramall David G. , Haque Abdul M.
Published: January 2024

CubeSats, known for their compact size and cost effectiveness, have gained significant popularity. However, their limited size imposes restrictions on the optical aperture and, consequently, the Ground Resolution Distance in Earth Observation missions. To overcome this limitation, the concept of deployable optical payloads with segmented primary mirrors which can unfold like petals has emerged, enabling larger synthetic apertures and enhanced spatial resolution. This study explores the potential benefits of leveraging Additive Manufacturing (AM) and Topology Optimization (TO) in the realm of ultra-precision machining, specifically single-point diamond machining. The goal is to reduce fixture weight while improving stiffness to minimize deformations caused by rotational and cutting forces which compromise optical performance. Through Finite Element Analysis, this research compares conventionally machined fixtures with those produced using AM and TO techniques. The results reveal that concept designs created via TO can achieve a remarkable 68% reduction in weight. This reduction makes the assembly, including the machining fixture and 12 U deployable segments, manageable by a single operator without the need for specialized lifting equipment. Moreover, these innovative designs lead to substantial reductions of up to 86% and 51% in deformation induced by rotational and cutting forces, respectively.
Effect of Different Herbage Allowances from Mid to Late Gestation on Nellore Cow Performance and Female Offspring Growth until Weaning
Authors: Sousa Luciana Melo , de Souza William Luiz , Oliveira Karla Alves , Cidrini Iorrano Andrade , Moriel Philipe , Nogueira Henrique César Rodrigues , Ferreira Igor Machado , Ramirez-Zamudio Germán Dario , Oliveira Ivanna Moraes de , Prados Laura Franco , Resende Flávio Dutra de , Siqueira Gustavo Rezende
Published: January 2024

This study evaluated different herbage allowances from mid to late pregnancy on pre- and postpartum physiological responses, milk production, and the performance of Nellore cows and the preweaning growth of their female offspring. Sixty multiparous Nellore cows were blocked by their body weight (BW; 425 ± 36 kg) and body condition score (BCS; 3.67 ± 0.23, scale 1–5) and randomly allocated to twelve pastures. Treatments consisted of two different herbage allowances (HA) during pregnancy: low HA (LHA; 2.80 kg DM/kg of BW) and high HA (HHA; 7.60 kg DM/kg of BW). Both treatment groups were fed 1 g/kg BW of a protein supplement. After calving, all cow–calf pairs were combined in a single group. The effects of maternal treatment × day of the study were detected for herbage mass and allowance, the stocking rate and forage crude protein, and for cow BW, BCS, and carcass measures (p < 0.01). Milk yield corrected to 4% fat, while the levels of fat total solids and cow plasma IGF-1 and urea were different (p ≤ 0.04) between treatments. HHA offspring was heavier (p ≤ 0.05) at 120 days and at weaning. A high herbage allowance can be implemented from mid-gestation until calving to increase cow prepartum performance, post-partum milk yield and composition, and positively modulate female offspring preweaning growth.
Egg Quality, Yolk Fatty Acid Profiles from Laying Hens Housed in Conventional Cage and Cage-Free Production Systems in the Andean Tropics
Authors: Rodríguez-Hernández Roy , Rondón-Barragán Iang Schroniltgen , Oviedo-Rondón Edgar O.
Published: January 2024

Egg consumers worldwide have increased their concerns about laying hens’ welfare and its impact on final egg product quality. This study compared the egg quality parameters under the conventional cage (CC) and cage-free (CF) egg production systems in the tropics. The study was conducted on a commercial farm in Colombia using Hy-Line Brown pullets, reared under the same conditions for the first 15 wks. At 16 wks, the hens were distributed into two housing systems, CC and CF, on the same farm. The hens were fed the same diet for each phase in both systems and feed intake varied slightly. Egg samples were collected every six wks, from 22 to 82 wks of age. A total of 3960 eggs were analyzed at 11 sampling times. Parameters such as albumen height, egg weight, yolk color, eggshell thickness, eggshell strength, and Haugh units were determined using a DET-6000 machine. At 22 and 82 wks, screening for Salmonella spp. status was conducted using environmental and egg samples. Additionally, at 34, 64, and 82 wks, yolk samples were obtained for fatty acid profiles and crude protein (CP) analysis. The data were analyzed in a completely randomized block design with repeated measures (11 times): mean separation by Student’s t-test yolk pigmentation, Haugh Units, and albumen height (p < 0.001) were higher in the CF compared with the CC between 38 and 69 wks of age, and eggs at 63 and 82 wks (p < 0.05) were heavier in the CF compared to the CC. Likewise, eggs from the CC had better eggshell strength from 57 to 82 wks. In the egg yolk fatty acid profile at the 34th wk, the pentadecanoic, palmitic, and heptadecanoic acids had higher concentrations in the CF systems than the CC. At the 64th wk, the egg yolk fatty acids—lauric, myristic, and heptadecanoic—had higher concentrations in the CF; likewise, at the 82nd wk, egg yolks from the CC had higher concentrations of lauric, heptadecanoic, and nervonic fatty acids than the CF. The eggs and environmental samples were negative for Salmonella spp. throughout the whole production phase. These results indicated that the production system might impact internal and external egg quality measures, potentially due to various stressors, including environmental factors or behavior restrictions.
Identification and Analysis of Genes Related to Testicular Size in 14-Day-Old Piglets
Authors: Zhao Yunjiao , Zhang Liangzhi , Wang Lei , Zhang Jianbo , Shen Wenjuan , Ma Yuhong , Ding Chengxiang , Wu Guofang
Published: January 2024

The RNA-Seq technology was used to screen the key genes that affect the early development of the testes of Duroc × Landrace × Yorkshire piglets, to determine the regulatory pathway and provide reference for subsequent reproductive performance research, breeding, and other production practices. This study selected 14-day-old Duroc × Landrace × Yorkshire piglets as the trial animals. Testes from piglets with similar weights and no pathological changes were divided into small testis (ST) and large testis (LT) groups, and the RNA-Seq screening of differentially expressed genes (DEGs) was performed to find candidate genes and regulatory pathways related to early testicular development. The results show that 570 DEGs were found in the ST and LT groups, with 281 upregulated and 289 downregulated. The DEGs were mainly enriched on 47 gene ontology (GO) functional items. The Kyoto encyclopedia of genes and genotypes (KEGG) enrichment analysis found that there were 44 significantly enriched KEGG signal pathways, and the regulation of testicular development mainly focused on the arachidonic acid metabolism, Wnt signaling pathway and GnRH secretion pathways. The PTGES, SFRP1, SPP1, PLA2G4E, KCNJ5, PTGS2, and HCN1 genes were found to be as closely related to the testicular development of these Duroc × Landrace × Yorkshire piglets, and the differential gene expression was consistent with the real-time quantitative reverse transcription PCR (real-time qRT-PCR) validation results. This study was validated by high-throughput sequencing analysis and real-time qRT-PCR, and showed that the PTGES, SFRP1, SPP1, PLA2G4E, KCNJ5, PTGS2, and HCN1 genes may be involved in the regulation of germ cell development, spermatogenesis and semen traits. These should be further studied as candidate genes for early testicular development and reproductive trait regulation in boars.
The Genetic Selection of HSPD1 and HSPE1 Reduce Inflammation of Liver and Spleen While Restraining the Growth and Development of Skeletal Muscle in Wuzhishan Pigs
Authors: Ren Yuwei , Wang Feng , Sun Ruiping , Zheng Xinli , Liu Yuanyuan , Lin Yanning , Hong Lingling , Huang Xiaoxian , Chao Zhe
Published: January 2024

Wuzhishan (WZS) pigs, which are minipigs native to Hainan Province in China, are characterized by strong resistance to extreme hot temperatures and humidity. The relationship between their immune response and growth still needs to be clarified. In this study, we used whole genome sequencing (WGS) to detect variations within 37 WZS pigs, 32 Large White (LW) pigs, and 22 Xiangxi black (XXB) pigs, and ~2.49 GB of SNPs were obtained. These data were combined with those of two other pig breeds, and it was found that most of the genes detected (354) were located within the distinct genetic regions between WZS pigs and LW pigs. The network that was constructed using these genes represented a center including 12 hub genes, five of which had structural variations (SVs) within their regulatory regions. Furthermore, RNA-seq and RT-qPCR data for 12 genes were primarily consistent in liver, spleen, and LDM tissues. Notably, the expression of HSPs (HSPD1 and HSPE1) was higher while that of most genes involved in the JAK3-STAT pathway were lower in liver tissue of WZS pigs, compared with LW pigs. This likely not only reduced inflammation-related immune response but also impaired their growth. Our findings demonstrated the role of HSPs in the connection between inflammation and growth rate, while also providing the fundamental genetic selection of the adaptability of WZS pigs.
Influencing Mechanism of Tidal Disasters on Locust Breeding Area Evolution in the Eastern Coastal Area of China during the Ming and Qing Dynasties
Authors: Feng Di , Li Gang , Feng Chenxi , Wang Shuo , Nie Qifan , Wang Xingxing
Published: January 2024

Locust plagues and tidal disasters are primary natural hazards in China’s eastern coastal regions, yet their interrelationship remains unclear. This study, drawing on historical documents from the Ming and Qing dynasties (1368–1911 AD), focuses on Zhejiang Province and its Hangzhou Bay coastline, areas typically affected by tidal disasters. Employing advanced quantitative analysis and spatiotemporal models, the research aims to reveal the mechanisms behind tidal disasters and their impact on locust population dynamics. The findings indicate a limited spatiotemporal correlation between locust plagues and tidal or drought disasters but a significant association with flooding. The relationship between locust infestations and floods is notably strong in the unique geographical context of Hangzhou Bay’s northern shore. The ‘hydromarginal’ nature of the north coast creates an ideal habitat for locusts. This study pioneers in identifying flooding as a crucial mediator between tidal disasters and locust plagues, shedding light on the ‘typhoon-tidal-flood-locust’ disaster sequence and offering new insights into understanding and mitigating natural disasters in the region. In this study, we primarily employ a quantitative methodology, utilizing advanced data analysis and sophisticated spatiotemporal modeling to investigate the interplay between locust plagues and tidal disasters. Although some progress has been made in the study of historical natural disasters, systematic studies of the relationship between tidal floods and locust breeding sites along the east coast of China during the Ming and Qing dynasties are still scarce. This study fills this gap by employing advanced GIS and time series analysis techniques, combining traditional historical documentary studies with modern scientific methods and providing a new methodological approach to the analysis of historical disaster patterns.
Neurobiological Underpinnings of Hyperarousal in Depression: A Comprehensive Review
Authors: Xie Musi , Huang Ying , Cai Wendan , Zhang Bingqi , Huang Haonan , Li Qingwei , Qin Pengmin , Han Junrong
Published: January 2024

Patients with major depressive disorder (MDD) exhibit an abnormal physiological arousal pattern known as hyperarousal, which may contribute to their depressive symptoms. However, the neurobiological mechanisms linking this abnormal arousal to depressive symptoms are not yet fully understood. In this review, we summarize the physiological and neural features of arousal, and review the literature indicating abnormal arousal in depressed patients. Evidence suggests that a hyperarousal state in depression is characterized by abnormalities in sleep behavior, physiological (e.g., heart rate, skin conductance, pupil diameter) and electroencephalography (EEG) features, and altered activity in subcortical (e.g., hypothalamus and locus coeruleus) and cortical regions. While recent studies highlight the importance of subcortical–cortical interactions in arousal, few have explored the relationship between subcortical–cortical interactions and hyperarousal in depressed patients. This gap limits our understanding of the neural mechanism through which hyperarousal affects depressive symptoms, which involves various cognitive processes and the cerebral cortex. Based on the current literature, we propose that the hyperconnectivity in the thalamocortical circuit may contribute to both the hyperarousal pattern and depressive symptoms. Future research should investigate the relationship between thalamocortical connections and abnormal arousal in depression, and explore its implications for non-invasive treatments for depression.
Strength Estimation and Feature Interaction of Carbon Nanotubes-Modified Concrete Using Artificial Intelligence-Based Boosting Ensembles
Authors: Zhu Fei , Wu Xiangping , Lu Yijun , Huang Jiandong
Published: January 2024

The standard approach for testing ordinary concrete compressive strength (CS) is to cast samples and test them after different curing times. However, testing adds cost and time to projects, and, therefore, construction sites experience delays. Because carbon nanotubes (CNTs) vary in length, composition, diameter, and dispersion, experiment and formula fitting alone cannot reliably predict the strength of CNTs-based composites. For empirical equations or traditional statistical approaches to properly forecast complex materials’ mechanical characteristics, various significant parameters, databases, and nonlinear relationships between variables must be considered. Machine learning (ML) tools are the most advanced for accurate predictions of material behaviour. This study employed gradient boosting, light gradient boosting machine, and extreme gradient boosting techniques to forecast the CS of CNTs-modified concrete. Also, in order to explore the influence and interaction of various features, an interaction analysis was conducted. In terms of R2, gradient boosting, light gradient boosting machine, and extreme gradient boosting models proved their accuracy. Extreme gradient boosting had the highest R2 of 0.97, followed by light gradient boosting machine and gradient boosting with scores of 0.94 and 0.93, respectively. This type of research may help both academics and industry forecast material properties and influential elements, thereby reducing lab test requirements.
Neuronal Autophagy: Regulations and Implications in Health and Disease
Authors: Liénard Caroline , Pintart Alexandre , Bomont Pascale
Published: January 2024

Autophagy is a major degradative pathway that plays a key role in sustaining cell homeostasis, integrity, and physiological functions. Macroautophagy, which ensures the clearance of cytoplasmic components engulfed in a double-membrane autophagosome that fuses with lysosomes, is orchestrated by a complex cascade of events. Autophagy has a particularly strong impact on the nervous system, and mutations in core components cause numerous neurological diseases. We first review the regulation of autophagy, from autophagosome biogenesis to lysosomal degradation and associated neurodevelopmental/neurodegenerative disorders. We then describe how this process is specifically regulated in the axon and in the somatodendritic compartment and how it is altered in diseases. In particular, we present the neuronal specificities of autophagy, with the spatial control of autophagosome biogenesis, the close relationship of maturation with axonal transport, and the regulation by synaptic activity. Finally, we discuss the physiological functions of autophagy in the nervous system, during development and in adulthood.
Nanorods Assembled Hierarchical Bi2S3 for Highly Sensitive Detection of Trace NO2 at Room Temperature
Authors: Yang Yongchao , Liu Chengli , Wang You , Hao Juanyuan
Published: January 2024

The bismuth sulfide nanostructure has become a promising gas sensing material thanks to its exceptional intrinsic properties. However, pristine Bi2S3 as a room-temperature sensing material cannot achieve the highly sensitive detection of ppb-level NO2 gas. Herein, 1D nanorods with self-assembled hierarchical Bi2S3 nanostructures were obtained via a simple hydrothermal process. The as-prepared hierarchical Bi2S3 nanostructures exhibited outstanding NO2 sensing behaviors, such as a high response value (Rg/Ra = 5.8) and a short response/recovery time (τ90 = 28/116 s) upon exposure to 1 ppm NO2. The limit of detection of hierarchical Bi2S3 was down to 50 ppb. Meanwhile, the sensor exhibited excellent selectivity and humidity tolerance. The improved NO2 sensing properties were associated with the self-assembled hierarchical nanostructures, which provided a rich sensing active surface and accelerated the diffusion and adsorption/desorption processes between NO2 molecules and Bi2S3 materials. Additionally, the sensing response of hierarchical Bi2S3 nanostructures is much higher at 100% N2 atmosphere, which is different from the chemisorption oxygen model.