bluuebunny/crossref_metadata_2025 huggingface dataset:
Approximating Permeability of Microcomputed-Tomography Images Using Elliptic Flow Equations
Authors: Chung T.. , Wang Y. D. , Armstrong R. T. , Mostaghimi P..
Published: February 2019

Direct simulation of flow on microcomputed-tomography (micro-CT) images of rocks is widely used for the calculation of permeability. However, direct numerical methods are computationally demanding. A rapid and robust method is proposed to solve the elliptic flow equation. Segmented micro-CT images are used for the calculation of local conductivity in each voxel. The elliptic flow equation is then solved on the images using the finite-volume method. The numerical method is optimized in terms of memory usage using sparse matrix modules to eliminate memory overhead associated with both the inherent sparsity of the finite-volume two-point flux-approximation (TPFA) method, and the presence of zero conductivity for impermeable grain cells. The estimated permeabilities for a number of sandstone and carbonate micro-CT images are compared against estimation of other solvers, and results show a difference of approximately 11%. However, the computational time is 80% lower. Local conductivity can furthermore be assigned directly into matrix voxels without a loss in generality, hence allowing the pore-scale finite-volume solver (PFVS) to be able to solve for flow in a permeable matrix as well as open pore space. This has been developed to include the effect of microporosity in flow simulation.
Different managements of vertical gas breakthrough in femtosecond laser assisted LASIK
Authors: Wei Chenghua , Zhang Pengfei , Ge Yao , Mei Lixin , Song E
Published: February 2019

Background

To describe the different management of vertical gas breakthrough during FSL-LASIK.

Cases presentation

We present three cases that experienced a vertical gas breakthrough (VGB) during the flap creation for femtosecond laser assisted laser in situ keratomileusis (FSL-LASIK). All the cases were remedied using a femtosecond laser without the occurrence of adverse events during the second flap creation.

Conclusions

FSL-LASIK could be performed as a remedy for VGB in patients without obvious corneal lesions.
Load monitoring on Pilates training: a study protocol for a randomized clinical trial
Authors: Cavina Allysiê P. S. , Junior Eduardo Pizzo , Machado Aryane Flauzino , Biral Taise Mendes , Pastre Carlos Marcelo , Vanderlei Franciele Marques
Published: February 2019

Background: Currently there are campaigns to raise the awareness of the need to practice some physical exercise with several objectives, mainly as a preventive character. From this perspective, we can see the use of the Pilates method as an instrument of therapeutic exercise for the protection and promotion of health. However, despite being popularly performed, there is still no scientific evidence on the standardization of the use of the method and its progression to an adequate prescription of physical training. Therefore, the purpose of the study was to develop a protocol to monitor the progression of Pilates loads daily between the basic, intermediate and advanced levels, as well as to analyze the effect of the method on the psychometric, cardiorespiratory and autonomic parameters. Methods: there will be a total of 36 sessions of Pilates mat for 32 healthy men. In each training session, initially, cardiorespiratory parameters, pain through the Visual Analogue Scale (VAS), and a psychometric questionnaire will be collected for the volunteers. Heart rate (HR), subjective perception of effort (SPE), and RR intervals will be measured during the sessions for subsequent use to analyze the progression of the loads by monitoring the internal training load and heart rate variability (HRV), respectively. At the end of the sessions, cardiorespiratory parameters, the VAS, the psychometric questionnaire will be measured again and the participants will only be released after 15 minutes of rest for the final HR analysis and to re-respond to the PSE scale. Before and after the 36 sessions of training, participants will also be evaluated in relation to psychometric, cardiorespiratory, and autonomic parameters. Discussion: this study deserves to be highlighted as it is a parallel randomized clinical trial with standardization of training, with the purpose of monitoring the prescription of loads of the method, as well as verifying its efficacy in clinical, cardiorespiratory, and autonomic outcomes. The easy reproducibility of the protocol from its description, also improves the study, besides providing support for prescribing the method to the professionals involved.
A Comparison Between Scalp Nerve Block and Local Anesthetic Infiltration on the Inflammatory Response, Hemodynamic Response, and Postoperative Pain in Patients undergoing Craniotomy for Cerebral Aneurysms: A Randomized Controlled Trial
Authors: Yang Xi , Ma Jing , Chen Lei , Dong Rui , Li Ke , Lu Yayuan , Zhang MD Zongze , Peng MD Mian
Published: February 2019

BACKGROUND

The purpose of this study was to compare the effects of the scalp nerve block (SNB) and local anesthetic infiltration (LA) with ropivacaine 0.75% on postoperative inflammatory response, intraoperative hemodynamic response, and postoperative pain control in patients undergoing craniotomy.
The Role of Toll-Like Receptor 4 Mediates Microglial Activation during Remifentanil-Induced Hyperalgesia in Rats
Authors: chen xiangxiang , Wei Xin
Published: February 2019

Background: Opioids can induce a state of nociceptive sensitization, also known as opioid-induced hyperalgesia. Nevertheless, the exact mechanism is still unclear. The following study investigates the role of Toll-like receptor 4 (TLR4) in the microglia activation during remifentanil—induced hyperalgesia in rats’ model of incisional pain.
Methods: Mechanical allodynia induced by remifentanil was established in adult male Sprague–Dawley rats with incisional pain. Paw withdrawal threshold (PWT) and paw withdrawal thermal latency (PWTL) were performed to evaluate mechanical and thermal hyperalgesia. The 32-G catheter intrathecal placement was used to deliver a specific TLR4 antagonist (LPS-RS).Western blot analysis was performed to measure the expression of the TLR4 and Iba-1, while Immunoﬂuorescence staining was used to investigate the cell type and cell activation.
Results：Incisionalpain-remifentanil decreased the PWT and PWTL, upregulated the expression of TLR4 and microglia activation in the spinal cord. On the contrary, the intrathecal delivery of LPS-RS at the dose of 25 μg significantly decreased mechanical allodynia and prevented the upregulation of TLR4 induced by incisional pain-remifentanil
Conclusion: These findings suggest that TLR4 signaling pathway has an important role in incisional pain-remifentanil hyperalgesia, and that it could serve as the therapeutic target for persistent postsurgical pain
The rare and forbidden: Testing physics beyond the standard model with Mu3e
Authors: Perrevoort Ann-Kathrin
Published: February 2019

The upcoming Mu3e experiment aims to search for the lepton flavour
violating decay
\boldsymbol{\muposeeemath}
with an unprecedented final sensitivity of one signal decay in
\boldsymbol{\num{e16}}
observed muon decays by making use of an innovative experimental design
based on novel ultra-thin silicon pixel sensors. In a first phase, the
experiment is operated at an existing muon beam line with rates of up to
\boldsymbol{\num{e8}}
muons per second. Detailed simulation studies confirm the feasibility of
background-free operation and project single event sensitivities in the
order of
\boldsymbol{\num{e-15}}
for signal decays modelled in an effective field theory
approach. The precise tracking of the decay electrons and large
geometric and momentum acceptance of Mu3e enable searches for physics
beyond the Standard Model in further signatures. Examples of which are
searches for lepton flavour violating two-body decays of the muon into
an electron and an undetected boson as well as for electron-positron
resonances in
\boldsymbol{\muposeeenunumath}
which could result for instance from a dark photon decay. The Mu3e
experiment is expected to be competitive in all of these channels
already in phase I.
Determination of Evolution Stages of Landform: A Comparative Study of Mountain-Plain (Kosi, Bihar) and Plateau-Plain (Kangsabati, WB) Regions of Tropical India
Authors: Mahala Avijit , Pathak Varuni
Published: February 2019

The major aim of quantitative geomorphology is to understand the geomorphological stages of evolution of any area. The quantitative revolution and the development of remote sensing and GIS techniques have brought greater attention to this field of analysis. We have used a morphometric analysis, which is an important indicator to understand the geomorphic stages of evolution of any drainage basin, to compare the drainage basin characteristics and related stages of evolution of a mountain-plain and plateau-plain drainage basin in tropical India. The Kosi basin has been selected for the mountain-plain area and Kangsabati basin is the chosen region for the plateau-plain area. Different drainage morphometric parameters and measurements related to linear, areal, relief characteristics have been determined through the use of SRTM (Shuttle Radar Topographic Mission) GeoDEM and ARC GIS 10.1. Area-altitude relationship and hypsometric characteristics have also been accessed to identify the stages of geomorphic evolution. All the relief characteristics indicate Kosi in a young or rejuvenated stage when compared to the mature plateau region of the river of Kangsabati. Morphometric characteristics also indicate that there are high geologic and geomorphological controls on river basin characteristics.
A Biodiversidade na Era da Globalização: Mercado Internacional e Degradação Ambiental como Vetores de Simplificação da Biosfera
Authors: Neves Frederico Monteiro , Brandão Guerreiro Barbosa Luciano Celso , Da Cunha de Oliveira Santos Neves Joanna Maria
Published: February 2019

Historicamente, a introdução de espécies exóticas nos territórios caracterizou a história humana, contribuindo para a expansão do sistema econômico. Entretanto, a globalização hegemônica atual, devido ao seu ritmo, intensidade e extensão da degradação ambiental que lhe é peculiar tem conduzido à introdução de espécies exóticas e à perda da biodiversidade em escalas inéditas. O objetivo deste trabalho é investigar os processos relacionados à globalização que contribuem para a intensificação do processo simplificação da biodiversidade do planeta. Para tanto, foi realizada pesquisa em bases de dados secundários e documentos, além de revisão da literatura. Os resultados indicam que com a intensificação dos processos relacionados à globalização, a partir de meados do século XX, está em curso um processo de homogeneização da biosfera sem precedentes na história do Planeta. Além das implicações evolutivas e ecológicas, a própria reprodução das sociedades humanas estaria comprometida em um contexto de menor diversidade biológica.
The Use of Statistical Analyses in Papers and Graduate Programs in the Environmental Sciences area in Brazil
Authors: Gonçalves Tatiel Venâncio , Silva Thâmara Machado e , Ferreira Rafael Batista , Ramalho Werther Pereira , Moraes Ronny José de , Arruda Filipe Viegas de , Lima Flávia Pereira
Published: February 2019

Environmental issues emerge in complex dimensions, which require an interdisciplinary framework in Environmental Sciences. Due to the diversity in statistic methods, the graduate programs need to update to form the environmental scientists. We test the hypothesis that QUALIS A1 Journals in the Environmental Science area use more complex statistical analyses. We describe the tests offered by graduate programs with PhD degree in the Environmental Sciences. 33.5% of 1560 papers evaluated, didn’t present statistical analysis. A1 Journals used more T-test, Chi-Square and Mann-Whitney than B1 Journals. There was no difference in the use of univariate, multivariate and Bayesian analyses. In Brazil there are 37 undergraduate programs in Environmental Sciences, of which 10 don’t offer statistics course. Of 38 courses offered, 73.7% provide only univariate statistics and 34.2% provide multivariate statistics. We conclude that the quality in papers doesn’t depend on the complexity of used statistical analyses, but on their theoretical framework.
Os Passadiços do Paiva: Estudo Exploratório do seu Impacto Económico e Social
Authors: Oliveira Diana Saraiva , Tavares Fernando Oliveira , Pacheco Luís Dias
Published: February 2019

Este artigo tem como objetivo avaliar o impacto económico e social dos Passadiços do Paiva na Região Norte de Portugal. O método de análise utilizado é quantitativo e exploratório. O turismo natureza preocupa-se com comportamentos ambientais responsáveis, que promovem a educação ambiental e a equidade entre gerações, tendo por base um modelo de desenvolvimento sustentável e procurando o melhoramento da qualidade de vida das populações locais. Dos resultados obtidos através de 300 respostas ao inquérito, pode-se concluir que este produto turístico pode ser integrado num plano estratégico para o desenvolvimento da região e constituir um fator chave para a captação de investimento.
Indicador Sintético de Prácticas Ambientales de los Hogares: Caso de Estudio Cantón Loja, Ecuador
Authors: Toledo-Macas Elisa Evelyn , Saraguro-Viteri Mónica Jacqueline
Published: February 2019

Resumen
El objetivo de esta investigación es calcular un Indicador Sintético de Medio Ambiente (ISMA), que refleje el comportamiento ambiental de los hogares del cantón Loja en el año 2014. La metodología utilizada se divide en tres etapas, en la primera se normalizó las seis dimensiones de prácticas ambientales (ahorro de agua, ahorro de energía, eliminación de residuos habituales y ocasionales, transporte y movilidad y actitud medioambiental), en la segunda se ponderó las dimensiones a través del método Delphi y finalmente se realizó el cálculo del indicador de cada una de las dimensiones y el ISMA. Los resultados obtenidos permiten concluir que las prácticas ambientales que realizan los hogares lojanos son adecuadas y óptimas para el medio ambiente, el mismo que se refleja por un ISMA de nivel medio. Las dimensiones que tienen mayor aporte son el ahorro de agua, energía, eliminación de residuos habituales y actitud medioambiental, a diferencia de la eliminación de residuos ocasionales y transporte y movilidad, los cuales presentan valores no aceptables.
Creation of the 'Happiness 360º' Model: Qualitative Analysis from Survey Responses
Authors: Ribeiro Diamantino José Teixeira , Laranjeira Erika , Remondes Jorge
Published: February 2019

Qualitative data analysis has been increasingly important in the development of research, especially in the author’s main fields of investigation: Happiness Economics and Communication for the Development. To understand what Happiness means and in what form certain variables like gender, literacy, etc., influence the concept of happiness, based on a survey conducted in April 2018, we have analyzed the answers to the question "What is happiness for you? " using the software of Qualitative Analysis, Webqda. The great peculiarity is that it is an open question. It could be considered that the question is recurrent and the work redundant, but the results are very interesting from an analytical point of view. The 360º Happiness model main purpose is to support organizations in understanding the expectations of their workforce and help on defining their human resources management policies and adapt them according to the gender, generation or education level, for instance.
Acetate Kinase (AcK) is Essential for Microbial Growth and Betel-derived Compounds Potentially Target AcK, PhoP and MDR Proteins in M. tuberculosis, V. cholerae and Pathogenic E. coli: An in silico and in vitro Study
Authors: Tiwari Sandeep , Barh Debmalya , Imchen M. , Rao Eswar , Kumavath Ranjith K. , Seenivasan S. Prabu , Jaiswal Arun K. , Jamal Syed B. , Kumar Vanaja , Ghosh Preetam , Azevedo Vasco
Published: February 2019

Background:

Mycobacterium tuberculosis, Vibrio cholerae, and pathogenic Escherichia coli
are global concerns for public health. The emergence of multi-drug resistant (MDR) strains of these
pathogens is creating additional challenges in controlling infections caused by these deadly bacteria. Recently,
we reported that Acetate kinase (AcK) could be a broad-spectrum novel target in several bacteria
including these pathogens.

Methods:

Here, using in silico and in vitro approaches we show that (i) AcK is an essential protein in
pathogenic bacteria; (ii) natural compounds Chlorogenic acid and Pinoresinol from Piper betel and
Piperidine derivative compound 6-oxopiperidine-3-carboxylic acid inhibit the growth of pathogenic E.
coli and M. tuberculosis by targeting AcK with equal or higher efficacy than the currently used antibiotics;
(iii) molecular modeling and docking studies show interactions between inhibitors and AcK that
correlate with the experimental results; (iv) these compounds are highly effective even on MDR strains
of these pathogens; (v) further, the compounds may also target bacterial two-component system proteins
that help bacteria in expressing the genes related to drug resistance and virulence; and (vi) finally, all the
tested compounds are predicted to have drug-like properties.

Results and Conclusion:

Suggesting that, these Piper betel derived compounds may be further tested
for developing a novel class of broad-spectrum drugs against various common and MDR pathogens.
Neurokinin-1 Receptor Antagonists as Anticancer Drugs
Authors: Muñoz Miguel , Coveñas Rafael
Published: February 2019

Background::

Human tumor cells lines and tumor samples overexpress the neurokinin-1 receptor (NK-1R). Substance P (SP), after binding to NK-1Rs, induces tumor cell proliferation, an antiapoptotic effect and promotes angiogenesis and the migration of cancer cells for invasion and metastasis.

Methods: :

In contrast, NK-1R antagonists block the previous pathophysiological actions mediated by SP. These antagonists promote the death of tumor cells by apoptosis. Peptide and non-peptide NK-1R antagonists have been reported.

Results: :

Peptide NK-1R antagonists show chemical modifications of the SP molecule (L-amino acids being replaced by D-amino acids), whereas non-peptide NK-1R antagonists include numerous compounds with different chemical compositions while showing similar stereochemical features (affinity for the NK- 1R). Currently, there are more than 300 NK-1R antagonists.

Conclusion::

In combination therapy with classic cytostatics, NK-1R antagonists have additive or synergic effects and minimize the side-effects of cytostatics. The effect of NK-1R antagonists as broad-spectrum anticancer drugs is reviewed and the use of these antagonists for the treatment of cancer is suggested.
Biology-oriented Drug Synthesis (BIODS), Structural Characterization and Bioactivities of Novel Albendazole Derivatives
Authors: Khan Momin , Khan Shahid , Salar Uzma , Khan Khalid Mohammed , Rehman Gauhar , Gul Naeem , Khan Iltaf
Published: February 2019

Background:

Albendazole is a drug, belongs to the family of benzimidazole, and used as an anthelmintic agent in both human and veterinary medicine. It is marketed as Albenza which is used for the treatment of a variety of parasitic worm infestations such as roundworms, tapeworms, and flukes. In recent past, we have reported various classes of compounds as anti-glycating agents, in continuation of Biology-oriented Drug Synthesis (BIODS), seventeen albendazole derivatives 2-18 were synthesized evaluated for yeast glucose uptake activity.

Methods:

In the present study, Albendazole (2 g, 7.5 mmol), potassium hydroxide (3 g) were dissolved in ethanol (50 mL) into a 250 mL round-bottomed flask and refluxed for 48 h. TLC (ethyl acetate: hexane, 6:4) was monitored in order to check the reaction progress. After completion, the reaction mixture was dried under air and washed with an excess of distilled water. Precipitates were dried and crystallized from ethanol. The product was characterized by EI-MS and 1H-NMR.

Results:

Our present study showed that all compounds showed a varying degree of yeast glucose uptake activity ranging between IC50 = 51.41-258.40 µM, compared with standard metronidazole (IC50 = 41.86 ± 0.09 µM). This study has identified a series of potential leads for anti-glycating agents.

Conclusion:

Biology-oriented drug synthesis and in vitro yeast glucose uptake activity of albendazole derivatives gave rise to a number of lead molecule such as 3 (IC50 = 59.37 ± 0.26 µM), 5 (IC50 = 59.70 ± 0.32 µM), 6 (IC50 = 60.78 ± 0.54 µM), 8 (IC50 = 54.61 ± 0.20 µM), 16 (IC50 = 56.57 ± 0.04 µM) and 14 (IC50 = 51.41 ± 1.25 µM).
Novel 3-{4-[2-Amino-4-(Substitutedphenyl)-2H-[1, 3] Oxazin/Thiazin-6-Yl} -2-Phenyl-3H-Quinazolin-4-One Derivatives as Enhancer of GABA Mediated Inhibition: Synthesis, Molecular Modeling and Pharmacological Studies
Authors: Jain Nimisha , Singour Pradeep Kumar
Published: February 2019

Background:

According to WHO, the 50 million people worldwide are suffering from epilepsy, making it one of the most common neurological diseases globally. Epilepsy is often characterized by neurobiological, cognitive, psychological and behavioral changes and that may enhance the susceptibility to seizures and affect the quality of life.

Objective:

The aim of the present work was to develop 2, 3 disubstituted 4-(3H)-quinazolinone derivatives in order to find an effective and highly lipophilic compound with lesser side effects and to evaluate them for anticonvulsant and neurotoxic activity.

Methods:

A novel series of 3-4-[2-amino-4-(substitutedphenyl)-2H-[1.3] oxazin/thiazin-6-yl 2- phenyl-3H-quinazolin-4-one derivatives were synthesized and evaluated for their anticonvulsant activity. The structures of the compound have been confirmed by spectral analysis. The molecular docking study was performed for finding the binding affinity with GABAA receptor in order to rationalize their anticonvulsant activities in a qualitative way. Quantitative estimate of drug-likeness was also performed which calculate the molecular properties and screen the molecules based on drug-likeness rules. Anticonvulsant activities of synthesized compounds were done by using (Maximal electroshock) MES induced seizures and subcutaneous pentylenetetrazole (scPTZ) induced seizure models in Wistar rats of either sex. None of the compounds demonstrated any sign of neurotoxicity.

Results:

Compounds 3-4-[2-amino-4-(fluorophenyl)-2H- [1, 3] oxazin-6-yl 2-phenyl-3H quinazolin-4-one (5i) and 3-4-[2-amino-4-(fluorophenyl)-2H- [1, 3] thiazin -6-yl 2-phenyl-3H quinazolin-4-one (5n) have shown significant activity against tonic seizure by the MES model and clonic seizure by scPTZ induced seizure model.

Conclusion:

These ten novels synthesized compounds had significant anticonvulsant activity. As a result, the compound (5i) and (5n) emerged out as the pilot molecule with a better anticonvulsant activity without any neurotoxicity, while the other compounds have moderate activity. QED analysis of compounds (5i) and (5n) also indicated that these compounds will have good oral absorption. The proposed work is to make efforts towards the development and identification of novel molecules as anticonvulsant agents by the synthesis of some novel quinazolinone derivatives with improved biological activity.
Characterization of an Unknown Impurity in Glucosamine Sulfate Sodium Chloride by HPLC-Q-TOF MS and NMR
Authors: Zhang Miao , Zhu Peixi , Chen Yue , Ni Weifang , Li Yu , Hong Liya
Published: February 2019

Background:

Glucosamine sulfate sodium chloride (glucosamine-SP) is mainly used for the
treatment of osteoarthritis. During quality control of glucosamine-SP capsules, an unknown impurity
was detected. Another unknown degradation product was generated together with above-mentioned
impurity in heat condition.

Objective:

The study aimed to characterize an unknown impurity in glucosamine-SP capsules.

Methods:

A new volatile HPLC method compatible with mass spectrometry detection was set up. An
amino column at 35 °C with a mobile phase consisting of water and acetonitrile (20: 80, v/v) was used
at a flow rate of 1.5 ml/min at 297 nm. High-performance liquid chromatography quadrupole time-offlight
mass spectrometry (HPLC-Q-TOF MS) was used to identify the impurity with the electrospray
ionization (ESI) source in the positive ionization mode.

Results:

The results of HPLC-Q-TOF MS analysis indicated that the protonated molecule ions [M + H]+
of the unknown impurity and the novel degradation product were both at m/z 287. Preparative LC method
was put into practice with a Prep-C18 column with a mobile phase consisting of water and acetonitrile
(99: 1, v/v) at a flow rate of 20.0 ml/min at 297 nm. The assignment of the 1D and 2D NMR signals
was performed for the unknown impurity. In addition, the formation of impurities was also studied.

Conclusion:

An unknown impurity and a degradation product in glucosamine-SP capsules were characterized.
They were assigned as (1R, 2S, 3R)-1-(5-((S, E)-3, 4-dihydroxybut-1-en-1-yl) pyrazin-2-yl)
butane-1, 2, 3, 4-tetraol and (1R, 2S, 3R)-1-(5-((S, Z)-3, 4-dihydroxybut-1-en-1-yl) pyrazin-2-yl) butane-
1, 2, 3, 4-tetraol.
Effect of Chitosan, Chitosan Nanoparticle, Anacyclus pyrethrum and Cyperus rotundus in Combating Plasmid Mediated Resistance in Periodontitis
Authors: Subbiah Usha , Elayaperumal Gokulalakshmi , Elango Sonaa , Ramanathan Arvind , Gita Bagavad , Subramani Karthikeyan
Published: February 2019

Background:

Chitosan, chitosan nanoparticle, ethanolic extracts of Anacyclus pyrethrum
root and Cyperus rotundus rhizome were evaluated against plasmid mediated multidrug resistance
of Enterococcus faecalis, Staphylococcus aureus and Bacillus sp. isolated from unstimulated
saliva of chronic periodontitis. The main aim of the current study centres the reduction of antibiotic
consumption and the development of natural compounds to combat multidrug resistance.

Methods:

Identification of bacteria, antimicrobial susceptibility, plasmid stability and plasmid curing
was carried out for the characterization of resistance plasmids.

Results:

E. faecalis showed 89% of sensitivity to chitosan nanoparticle, chitosan 81%, C. Rotundus
69% and A. pyrethrum 62% for S. aureus, with the MIC >100 µg/ml. In comparison with the
antimicrobials tested, maximum resistance to tetracycline (89%) for E. faecalis, is followed by
ampicillin (87%) and tetracycline (81%) for S. aureus. Meanwhile, chloramphenicol and tetracycline
(80%) for Bacillus sp. The plasmid stability for E. faecalis (20%), S. aureus (<4%) and Bacillus
sp. (16%). The order of stability corresponding to the broth media is LB ˃ BHI ˃ nutrient for
E. faecalis, S. aureus but similar in LB and nutrient broth for bacillus sp. The maximum plasmid
curing efficiency of chitosan for S. aureus (76%), Chitosan nanoparticle for E. faecalis (88%), A.
pyrethrum for S. aureus (73%), C. rotundus for E. faecalis (87%). The order of plasmid curing efficiency
is chitosan nanoparticle>C. rotundus>chitosan>A. pyrethrum.

Conclusion:

Chitosan, Chitosan nanoparticle, C. rotundus, and A. pyrethrum offered a greater potential
in eliminating plasmid mediated resistance acquired by periodontal pathogens, thus addressing
this crisis in dentistry.
Endogenous Repair System of Oxidative Damage of DNA
Authors: Sharma Anmol , Gupta Pawan , Prabhakar Pranav Kumar
Published: February 2019

DNA is one of the most important biomolecules of living cells which carries genetic information from generation to generation. Many endogenous and exogenous agents may disrupt the structure of DNA. Change in the cellular genome can lead to errors in replication, transcription and in protein synthesis. DNA damage occurs naturally or result from a metabolic and hydrolytic process which release some very active chemical entities like free radicals, Reactive Oxygen Species (ROS), Reactive Nitrogen Intermediate (RNI), Reactive Carbonyl Species (RCS), lipid peroxidation products and alkylating agents. Superoxide radical, hydroxyl radical and hydrogen peroxide cause a significant threat to cellular integrity by damaging the DNA, lipids, proteins and other biomolecules. Oxidative stress may be explained as a disturbance in the number of free radicals and our system’s ability to neutralize these free radicals. Imbalances in the normal redox potential can also lead to toxic effects via the generation of peroxides. Oxidation of DNA bases leads to the base damage, nick in the strand and break in the strand either single or double strand. Oxidative stress can also cause modifications in normal mechanisms of cell signaling. DNA mutation can result in a number of genetic abnormalities such as cancer, heart failure, Alzheimer’s disease, and depression. Human body has special protection in the form of antioxidant molecules and enzymes against these free radicals. Generation of ROS and its neutralization must be regulated to protect cells and signalling biomolecules from the deleterious effect of oxidative stress with the involvement of antioxidant systems, enzymes, and specific proteins. DNA repair system is a complex system which helps in the identification, removal of the wrong nucleotide and repairs them and as a result, the cell will produce correct and functional protein and active enzyme.
Kinetics and Mechanism of Permanganate Oxidation of ADA in Aqueous Perchlorate Solutions
Authors: Hassan Refat , Ibrahim Samia M.
Published: February 2019

Background:

ADA as N-(2-Acetemido) iminodiacetic acid has been widely used in preparation
of organic buffers and protein-free media for fibroblasts as chelating agent chicken embryo
fibroblasts as tridentate chelating agent ligand. However, a little attention has been focused to the behavior
of its oxidation in aqueous acidic solutions. Accordingly, a lack of information on the aqueous
chemistry of oxidation of ADA in acidic solutions was recognized. Therefore, we have prompted to
undertake the present work with the aims at shedding more light on the behavior of oxidation and the
nature of oxidation products. Again, it aims to compensate the lack of information about the aqueous
chemistry of ADA and the nature of electron-transfer for redox reactions involving permanganate ion
as a multi-equivalent oxidant.

Method:

A spectrophotometric investigation of oxidation of ADA by permanganate ion in aqueous
perchlorate solutions has been investigated.

Results:

Absorbance-time curves indicated the presence of two distinct stages for the oxidation process.
The experimental results indicated a decrease in the reaction rates with increasing the [H+], ionic
strength and F- ions. Again, the reaction rates were found to increase with increasing the added
Mn2+ and temperature.

Conclusion:

These results indicated that the oxidation process was of acid-inhibition nature and proceeding
by free-radical intervention mechanism. Again, the reactive species in the fast second stage
was the Mn4+ species formed in the initial stage. The activation parameters have been evaluated
from the temperature dependence of the rate constants. A tentative reaction mechanism was suggested
for such redox reaction.
The role of miRNA genes participating in VHL-HIF1α in clear cell renal cell carcinoma
Authors: Pavlov V. N. , Gilyazova I. R. , Izmailov A. A. , Klimentova E. A. , Sultanov I. R. , Bermishev M. A. , Akhmadeev Z. R. , Nurgalieva A. Kh. , Ishbulatova G. V. , Khusnutdinova E. K.
Published: February 2019

Introduction. Much attention in ccRCC development is paid to VHL-HIF1α pathway genes. Numerous genes involved in the pathogenesis of ccRCC are targets for miRNA. Alteration in the nature of interaction with miRNA binding site as a result of a single nucleotide substitution may promote change the expression of target genes involved in the genesis and development of tumors.Purpose of research. Analysis of the role of polymorphic variants in the miRNA binding sites of the VHL-HIF1α gene pathways in ccRCC development.Materials and methods. We used 225 DNA samples isolated from the venous blood of ccRCC patients who are hospitalized to the Clinic of the Bashkir State Medical University, and 298 healthy individuals. The genotyping of miRNA binding site polymorphisms in VHL-HIFα-dependent pathway genes (rs10982724 of the DEC1 gene, rs406271 of the TFRC gene, rs10491534 of the TSC1 gene, rs1642742 of the VHL gene, rs3025033 of the VEGFA gene) was performed using Taq-man assays.Results. The frequency distribution of alleles and genotypes of rs1642742 of the VHL gene showed that rs1642742 *GG is a marker of the increased risk for ccRCC. In addition, rs10491534 * C allele was found to be the marker for severe ccRCC (p = 0.044; OR = 1.72 (CI = 1.012-2.911)), and rs10491534 * TT genotype (p = 0.044; OR = 0.55; (95% CI = 0.31–0.98)) of the TSC1 gene was shown to be a protective marker for ccRCC of severe duration.Conclusions. The study indicated the association of miRNA binding sites polymorphisms with the risk of ccRCC development and severity of disease. However, further studies of the genes are needed to establish their functional significance and role in the pathogenesis of ccRCC.
Academician S.R. Mirotvortsev (1878 - 1949) – the Clinical Physician, the Teacher, the Urologist Innovator (to the 140 anniversary since birth)
Authors: Morgoshiia T. Sh.
Published: February 2019

The main milestones of life and S.R. Mirotvortsev’s creativity are given in article. The little-known facts from the biography of professor are noted. Rewarding with two gold medals for student’s works is analyzed: «Vessels and nerves of sinews of foot of the person» (in common with the student V.P. Vorobyov) and «Dislocations of bones of a wrist». It is noted that during the Russian-Japanese war spent 11 months in the besieged Port Arthur and remained with the wounded who were taken prisoner to Japanese there. From 1905 to 1914 worked in VMA at first under the leadership of professor S.P. Fedorov, then the assistant for professor V.A. Oppel. It is shown that since 1914 until the end of life worked in Saratov, occupying department of the general surgery, and since 1920 — faculty surgical clinic. From 1922 to 1928 the rector of the Saratov university. In the period of the Great Patriotic War was the chief surgeon of Saratov and the Saratov region. S.R. Mirotvortsev published about 150 scientific works on questions of clinical and field surgery. In work it is analyzed that he in 1908 offered original operation of change of ureters in a rectum, having defended the doctoral dissertation (1909) on this subject. It is noted that the broad popularity was gained by its researches about malignant new growths, in particular sarcomas of tubular bones. It developed methods of unilateral switching off of a large intestine, plasticity of a firm meninx the sealing gland, processing’s of burn wounds potassium permanganate solution. S.R. Mirotvortsev suggested to use haemostatic properties of a catgut for a local stop of bleedings from parenchymatous bodies and sine of a firm meninx. From numerous works on field surgery detailed statistical development of 180 thousand cases of a gunshot wound is especially valuable during World War I of 1914 — 1918. It is noted that the department of faculty surgery of the Saratov medical institute and the street in Saratov is called by the name of S.R. Mirotvortsev. It is awarded with awards of the Labour Red Banner, the Red Star and medals.
Pengaruh Kualitas Lingkungan Terhadap Pemenuhan Kebutuhan Dasar di Provinsi Banten
Authors: Suryani Anih Sri
Published: February 2019

The quality of the environment and basic needs of the community as long as it is a matter of concern and need to be improved. This study aims to measure the effect of environmental quality on the fulfillment of basic needs. Mixed methods of Quantitative (with questionnaires) and qualitative (by interview) are conducted in data collection and analysis. The results showed that all environmental quality indexes were correlated with the fulfillment of basic needs, except for the biodiversity index. Based on the correlation test it is found that all environmental quality index have positive effect on the fulfillment of basic needs. This means, if there is an increase in air quality, water quality, land cover, biodiversity, public health, and environmental health then the fulfillment of basic needs will increase, and vice versa. The air quality index and land cover have the largest constants among the other indices. Thus, an increase of that value will have a greater impact on the increase in basic compliance, and vice versa. Kualitas lingkungan dan kebutuhan dasar masyarakat selama ini merupakan hal yang menjadi perhatian dan perlu terus ditingkatkan. Penelitian ini bertujuan untuk mengukur pengaruh kualitas lingkungan terhadap pemenuhan kebutuhan dasar. Metode gabungan kuantitatif (dengan penyebaran kuesioner) dan kualitatif (dengan wawancara) dilakukan dalam pengumpulan dan analisis data. Hasil penelitian di Provinsi Banten menunjukkan bahwa semua indeks kualitas lingkungan berkorelasi dengan pemenuhan kebutuhan dasar, kecuali indeks keanekaragaman hayati. Berdasarkan uji korelasi didapatkan bahwa semua indeks kualitas lingkungan berpengaruh positif terhadap pemenuhan kebutuhan dasar. Hal ini berarti, apabila terjadi kenaikan kualitas udara, kualitas air, tutupan lahan, keanekaragaman hayati, kesehatan masyarakat, dan kesehatan lingkungan maka pemenuhan kebutuhan dasar akan meningkat, begitu juga sebaliknya. Indeks kualitas udara dan tutupan lahan mempunyai konstanta yang paling besar diantara indeks lainnya. Dengan demikian, kenaikan dari nilai tersebut akan berdampak lebih besar pada kenaikan pemenuhan dasar, demikian juga sebaliknya.
Apresentação
Authors: Pires Daniela De Oliveira , Peroni Vera Maria Vidal
Published: February 2019

Revista Práxis Educacional
O MARCO REGULATÓRIO DO TERCEIRO SETOR: A OFERTA DA EDUCAÇÃO INFANTIL NO MUNICÍPIO DE PORTO ALEGRE
Authors: Montano Monique Robain , Pires Daniela De Oliveira
Published: February 2019

Resumo: Este artigo apresenta alguns elementos para o debate sobre a construção do marco regulatório do Terceiro Setor, o qual estabelece um regime jurídico próprio para a realização das parcerias entre a administração pública e as organizações da sociedade civil na promoção dos direitos sociais. Para fins dessa análise, será apresentada a consecução da educação infantil entre o Município de Porto Alegre e as creches conveniadas. O recorte histórico-normativo tem início no final dos anos 1990, com a aprovação da Lei nº 9.637/98 e da Lei nº 9.790/99, no qual estabeleceu-se as figurasjurídicas das Organizações Sociais e das Organizações da Sociedade Civil de Interesse Público. Nos anos 2000, foi aprovado o Marco Regulatório do Terceiro Setor, por meio da Lei nº 13.019/14, que traz uma série de mudanças para a formalização de parcerias entre as Organizações da Sociedade Civil e a Administração Pública. Com relação específica ao contexto porto-alegrense, será analisada a elaboração do Decreto Municipal 19.775/2017, que regulamenta a aplicação da Lei Federal nº 13.019. A metodologia utilizada para o desenvolvimento do estudo foi a análise quantitativa e qualitativa. Verificamos, ao final, que as parcerias público-privada impulsionam um processo de reconfiguração do papel do Estado na execução das políticas públicas educacionais, com o repasse da responsabilidade no plano da execução para as entidades do Terceiro Setor na oferta da educaçãoinfantil, implicando negativamente na efetivação dos direitos, lesando princípios de equidade e justiçasocial.
O MARCO REGULATÓRIO E AS PARCERIAS PÚBLICO-PRIVADAS NO CONTEXTO EDUCACIONAL
Authors: Peroni Vera Maria Vidal , De Oliveira Cristina Maria Bezerra
Published: February 2019

Resumo: Nosso objetivo é analisar a parceria de uma instituição do terceiro setor – a Fundação Lemann – com o Estado, a partir das redefinições do Marco Regulatório (2015) frente às políticas sociais, o que provocou uma correlação de forças na definição do conteúdo e direção da proposta que interferem diretamente nas práticas pedagógicas e de gestão, no chão da escola. Metodologicamente, nosso trabalho explorou a pesquisa documental por meio da legislação do Marco Regulatório, bem como em informações disponibilizadas em sites da Fundação Lemann e análise de dados de outros sites, de seus parceiros. Selecionamos alguns elementos da parceria que a Fundação Lemann,instituição privada, sem fins lucrativos, tem apresentado como frentes de trabalho, na atuação de programas e projetos para as redes de ensino, municipais e estaduais, em todas as regiões do Brasil. A escolha por esta Fundação se deu por ser uma das mais novas instituições do terceiro setor e por conta de sua abrangência dentro e fora do país, influenciando políticas públicas de educação.
LO QUE SE ENSEÑANZA COMO YOGA, MINDFULNESS Y VIPASSANA EN LAS ESCUELAS POR EL MUNDO: DEBATES Y CONTROVERSIAS
Authors: Roach Eduardo Francisco Freyre , Lamar Adolfo Ramos , Zoboli Fabio Zoboli
Published: February 2019

Lo que se conoce hoy en día como yoga, mindfulness y vipassana con propósitos no religiosos, sino seculares, se ha extendido vertiginosamente por el mundo. Programas de esta índole se están introduciendo en las escuelas, prometiendo beneficios no solo para la salud, sino también espirituales: autoconocimiento, autocontrol, paz interior, conexión con el mundo, compasión, y sentido de realización plena y felicidad. Empero, cuando el programa docente se concibe en la perspectiva de la racionalidad económica neoliberal y corporativa, más bien tenderá a promover la espiritualidad del individualismo, el consumismo, la meritocracia, y la aceptación pasiva del status quo.
CURRÍCULO NA EDUCAÇÃO INFANTIL: O QUE PENSAM OS PROFESSORES?
Authors: Alves Francione Charapa , Fialho Lia Machado Fiuza
Published: February 2019

O objetivo deste trabalho é compreender a concepção de currículo constituída por professores de Educação Infantil da rede pública do município de Crato no Ceará. Utilizou-se estudo de caso desenvolvido por intermédio da metodologia do Discurso do Sujeito Coletivo, que sefundamenta na Teoria das Representações Sociais, com a atribuição de sentido de sujeitos para tratamento dos dados do tipo narrativas orais coletados em entrevistas semiestruturadas com nove professoras da Educação Infantil. A análise foi realizada a partir da criação de eixos discursivos organizados por grupos de atores sociais com base nas ancoragens para a criação do Discurso do Sujeito Coletivo. Constatou-se que há uma necessidade de ampliar a discussão sobre o currículo naescola, já que esta categoria não é percebida como um artefato social pelos professores e, muito embora eles a considerem um elemento importante, a compreensão é insipiente para fomentar um bom trabalhado na Educação Infantil.
ARTE, ATIVIDADE DE ENSINO E O DESENVOLVIMENTO DA CAPACIDADE CRIATIVA NA EDUCAÇÃO ESCOLAR
Authors: De Abreu Thiago Xavier
Published: February 2019

Resenha sobre o livro A criatividade na arte e na educação escolar: uma contribuição à pedagogia histórico-crítica à luz de Georg Lukács e Lev Vigotski, de Maria Cláudia da Silva Saccomani.
INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE
Authors: SÖYLEMEZ Saniye
Published: February 2019

Since carbon nanotubes and conjugated polymers promise to lead a number of real-world technologies, herein, an electrochemical biosensor, using poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-{2,1’,3}-thiadiazole)] (PFBTz) and multi-walled carbon nanotubes (MWCNTs) was constructed for glucose sensing. To create such a surface, a graphite electrode was covered with PFBTz and MWCNTs, respectively. Then, glucose oxidase (GOx) was immobilized onto this new platform using glutaraldehyde (GA) as the cross linker. The limit of detection, linear range and sensitivity values were found to be 0.018 mM, 0.025–0.6 mM and 66.0 μAmM-1cm-2, respectively. The selectivity of the prepared biosensor was also examined in the presence of interferences and as a result, it has been observed that the biosensor response was much higher for glucose in all measurements. Surface characterizations before and after enzyme immobilization were done using scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques. In the final part of the study, the constructed biosensor was applied to beverages for glucose detection and very promising results were obtained. With this work, a robust, novel and economic sensing platform was prepared for glucose determination.
Effects of Light Therapy on Mood and Insulin Sensitivity in Patients With Type 2 Diabetes and Depression: Results From a Randomized Placebo-Controlled Trial
Authors: Brouwer Annelies , van Raalte Daniel H. , Nguyen Hoang-Ton , Rutters Femke , van de Ven Peter M. , Elders Petra J.M. , Moll Annette C. , Van Someren Eus J.W. , Snoek Frank J. , Beekman Aartjan T.F. , Bremmer Marijke A.
Published: February 2019

OBJECTIVE

Depression is common in patients with type 2 diabetes and adversely affects quality of life and diabetes outcomes. We assessed whether light therapy, an antidepressant, improves mood and insulin sensitivity in patients with depression and type 2 diabetes.

RESEARCH DESIGN AND METHODS

This randomized, double-blind, placebo-controlled trial included 83 patients with depression and type 2 diabetes. The intervention comprised 4 weeks of light therapy (10,000 lux) or placebo light therapy daily at home. Primary outcomes included depressive symptoms (Inventory of Depressive Symptomatology [IDS]) and insulin sensitivity (M-value derived from the results of a hyperinsulinemic-euglycemic clamp). Secondary outcomes were related psychological and glucometabolic measures.

RESULTS

Intention-to-treat analysis showed that light therapy was not superior to placebo in reducing depressive symptoms (−3.9 IDS points [95% CI −9.0 to 1.2]; P = 0.248) and had no effect on insulin sensitivity (0.15 mg/kg*min [95% CI −0.41 to 0.70]; P = 0.608). Analyses incorporating only those participants who accurately adhered to the light therapy protocol (n = 51) provided similar results, but did suggest positive effects of light therapy on depression response rates (≥50% reduction in IDS points) (26% more response; P = 0.031). Prespecified analysis showed effect moderation by baseline insulin sensitivity (P = 0.009) and use of glucose-lowering medication (P = 0.023). Light therapy did not affect depressive symptoms in participants with higher insulin sensitivity or those who use only oral glucose-lowering medication or none at all, but it did produce a relevant effect in participants with lower insulin sensitivity (−12.9 IDS points [95% CI −21.6 to −4.2]; P = 0.017) and a trend toward effectiveness in those using insulin (−12.2 IDS points [95% CI −21.3 to −3.1]; P = 0.094). Light therapy was well tolerated.

CONCLUSIONS

Although this trial is essentially inconclusive, secondary analyses indicate that light therapy might be a promising treatment for depression among a subgroup of highly insulin-resistant individuals with type 2 diabetes.