bluuebunny/crossref_metadata_2025 huggingface dataset:
PSVIII-14 Fifteen months of using litter from recycled manure as bedding for dairy cows: a focus on Klebsiella pneumoniae, Klebsiella variicola and Clostridium perfringens
Authors: Julien Carl
Published: November 2020

In recent years, for economical purposes and to improve well-being conditions of cows, several dairy producers have adopted litter made from recycled manure solids (RMS). However, its use raises several safety issues, particularly with respect to mastitis and other infections. We previously observed cases of mastitis caused by K. pneumoniae and K. variicola, and two deadly infections from C. perfringens from cows on RMS. A herd of Holstein cows, distributed in two barns of about 40 lactating cows, was used. RMS was used in one barn, and wood-chip litter (WCL), in the other. RMS was generated by roller presses separation followed by conditioning at 55°C for 24h. Clean and soiled (used) RMS and WCL samples were collected for 15 months. Similarly, material samples before conditioning were also collected, and all samples were analyzed by viability-qPCR. Milk samples from cows with mastitis and somatic cell count data (SCC) were analyzed. Total and living contents of K. pneumoniae, K. variicola, C. perfringens and Klebsiella spp. were higher in the RMS than the WCL (+1.6–4.2 log10, P < 0.05). Composting 24h at 55°C did not affect total and live levels of C. perfringens, K. pneumoniae, and even increased total and live levels of K. variicola (+0.9 log10, P < 0.05). A seasonal effect or the number of cycles recycling the RMS decreased levels of K. pneumoniae in winter. Overall, RMS had no significant effect on SCC, but increased mastitis incidence (+39%, P < 0.05), compared to WCL. Milk analysis revealed that higher levels of K. pneumoniae found in RMS could contribute to the increase in mastitis cases caused by this pathogen. Data reported from this study are not favorable to the use of an RMS conditioned for 24h at 55°C with dairy cows, apropos the microbiological aspect; however, these results must be confirmed by other studies.
PSVIII-7 Dietary L-arginine supplementation enhances growth performance, intestinal antioxidant capacity, immunity and modulate gut microbiota in yellow feathered chickens
Authors: Ruan Dong , Fouad A M , Fan Qiuli , Jiang Shouqun
Published: November 2020

This study investigated the effects of dietary Arginine (Arg) on performance, intestinal antioxidant capacity, immunity, and gut microbiota in Chinese yellow-feathered chickens. One thousand and two hundred 1-day-old female Qingyuan partridge chickens were randomly assigned to 5 groups with 6 replicates of 40 birds each. Chickens were fed diets with 5 total Arg levels (8.5, 9.7, 10.9, 12.1 and 13.3 g/kg) without antibiotics for 30 D. Average daily feed intake, average daily gain, and feed conversion ratio were improved with dietary Arg levels (P < 0.05). Dietary Arg level had a linear (P < 0.05) or quadratic (P < 0.05) effect on the gene expression of glutathione peroxidase 1, hame oxygenase 1, nuclear factor erythroid 2, linearly and quadraticly increased activities of GSH-Px and total antioxidant capacity in the jejunum and ileum. In addition, relative expression of interleukin-1β and toll-like receptor 4 decreased linearly (P < 0.05) and quadratically (P < 0.05) in the ileum with increasing dietary Arg levels; secretory immunoglobulin A contents were increased. In addition, sequencing data of 16S rRNA indicated that dietary Arg increased the abundances of Firmicutes phylum, Romboutsia and Candidatus Arthromitus genus, while decreased that of Clostridium sensu stricto 1. A diet containing 12.1 g Arg/kg could promote growth performance, intestinal antioxidation, innate immunity and modulate gut microbiota in yellow feathered chickens.
PSI-21 Relationship of captive bolt stunning location with basic tissue measurements and exposed cross-sectional brain area in cadaver heads from market pigs
Authors: Anderson Karly , Ries Elizabeth , Backes Jacob , Bishop Katherine , Boll Miranda , Brantner Ellie , Hinrichs Brady , Kirk Ashlynn , Olsen Hannah , Risius Blake , Bildstein Charles , Vogel Kurt D
Published: November 2020

The objective of this study was to contrast the soft tissue thickness, cranial thickness, total tissue thickness, cross-sectional brain area and bolt–brain contact from the common frontal application of captive bolt euthanasia with the alternative location behind the ear in cadaver swine heads. Twenty-three cadaver heads from pigs that were approximately 136 kg and 6 mo of age were collected from a slaughter establishment following CO2 stunning and assigned to either the FRONTAL (n = 11) or CAUDAL TO PINNA (n = 12) application of the captive bolt. The soft tissue thickness was different (P < 0.0001) between the 2 applications (FRONTAL: 8.3±3.4 mm; CAUDAL TO PINNA: 56.5±3.4 mm). The cranial thickness was different (P < 0.0001) between the applications (FRONTAL: 23.4±2.9 mm; CAUDAL TO PINNA: 26.5±2.9 mm). There was a difference (P < 0.0001) in the total tissue thickness between the 2 applications (FRONTAL: 31.7±3.8 mm; CAUDAL TO PINNA: 73.4±3.8 mm). Cross-sectional area was calculated from images collected immediately after the heads were cut along the plane of bolt travel by bandsaw and was different (P = 0.0028) between the 2 applications (FRONTAL: 25.2±1.3 cm2; CAUDAL TO PINNA: 18.9±1.3 cm2). Bolt–brain contact was also assessed from the images, and a difference (P = 0.0360) between the 2 applications (FRONTAL: 100±10.5%; CAUDAL TO PINNA: 66.7±10.5%) was identified. The results of this study suggest that the FRONTAL application may provide a bolt path with less tissue to travel through when compared with the CAUDAL TO PINNA application for pigs of the approximate age and weight of those in this study. Ultimately, the FRONTAL location may present less risk for the captive bolt euthanasia of swine at market weight at this time. Additional refinement of the CAUDAL TO PINNA procedure and modification to the captive bolt device to penetrate to a suitable depth to ensure brain damage is recommended.
PSI-20 Public perceptions of problematic equine industry sectors and management scenarios
Authors: Melvin Kelly , Ivey Jennie L , Schneider Liesel G , Krawczel Peter
Published: November 2020

The equine industry is highly variable with many different sectors and management practices. To determine how the public views common management practices and discipline-specific areas of the equine industry, an online study was distributed via email and social media over a 6-week period to U.S. residents over the age of 18 (n = 1,372). Survey questions included demographics, industry connection, definition of welfare and equine classification. Respondents were asked to select the most concerning option from a series of management-related scenarios. The production livestock and equine industries were then segmented by species or discipline, respectively, and respondents were asked which sector was most problematic. To analyze the data, frequency tables (Proc FREQ) and multinomial logistic regression (Proc LOGISTIC) were used in SAS 9.4 (Cary, NC) to test the factors associated with likelihood to select a given management scenario from each series (α=0.05). Respondents who were heavily connected to the industry were four times more likely than lightly connected individuals to select that a blanketed horse or unblanketed horse in 30°F weather with unlimited access to food and water equally presented no concern than to say that a blanketed or unblanketed horse in 30°F weather with unlimited access to food and water and were concerning (OR= 4.09; 95%CL: 2.08,8.04). Of the 1,244 respondents who answered, 563 (45%) said that the gaited horse industry is the most problematic equine industry compared to the racing industry (41%) and stock horse industry (8.7%). Understanding how the public perceives the various animal industries and management scenarios in relation to an individual’s connection to the industry, classification of equines and welfare definition is important to assess and improve educational intervention strategies.
PSI-4 Changes in serum cortisol concentrations and cytokine profiles of growing pigs subjected to low-intensity heat stress
Authors: Anderson Lauren E , Holt Jonathan P , van Heugten Eric , Poole Daniel H
Published: November 2020

This study was to determine the impact of low-intensity heat stress on inflammatory biomarkers in serum, ileum, jejunum and lung lavage samples. Pigs (n = 64; 24.6 ± 3.7 kg BW) were housed four per pen in thermoneutral conditions (CON; 23.9°C ± 2.5°C) or constant high ambient temperatures (HS; 28.7°C ± 1.3°C) for 16 d, followed by thermoneutral conditions (24.4°C ± 1.8°C) for both groups through d 21, and subsequent heat stress (28.0°C ± 3.0°C) for both groups through d 30. Respiration rate (P < 0.01) was greater in HS than CON pigs from d 2 through d 15 (mean: 82.54 breaths/min vs. 59.26 breaths/min). HS pigs had greater cortisol concentration (40.1 ng/mL vs. 27.3 ng/mL) than CON on d 2, but lower concentrations on d 22 (32.9 ng/mL vs. 48.9 ng/mL) and d 25 (25.4 ng/mL vs. 42.0 ng/mL; treatment x day: P < 0.001). HS pigs tended to have greater serum IL1β (190.1 vs. 143.4 pg/mL; P = 0.06) and greater serum IL12 concentrations (914.9 vs. 785.4 pg/mL; P < 0.01) than CON pigs. HS pigs tended to have greater ileal mucosa IL6 concentrations than CON pigs (50.4 vs. 30.9 pg/mL; P = 0.08). Jejunal mucosa IL1α concentrations tended (P = 0.10) to be greater for CON than HS pigs (13.5 vs. 9.0 pg/mL) and CON pigs had greater jejunal IL12 concentrations (18.1 vs. 11.8 pg/mL) than HS pigs (P = 0.02). Bronchioalveolar lavage from HS pigs tended (P = 0.10) to have a greater granulocyte-macrophage colony-stimulating factor concentration (6.9 vs. 5.3 pg/mL) than CON pigs and had greater IL4 (7.7 vs. 4.2 pg/mL) and IL12 concentrations (34.8 vs. 24.5 pg/mL) than CON pigs (P = 0.01). Increased inflammatory activity and endocrine stress response occurred during low-intensity heat stress, indicating it may be more detrimental than previously accepted.
PSIV-19 Relationship between liver abscess microbiome of feedlot cattle and soil organic matter microbiome isolated from feedlot pens
Authors: Smith Kathryn E , Bester Zeno , Hubbert Michael E , Loest Clint A
Published: November 2020

Liver abscess (LA) prevalence has increased 15% for beef cattle and 25% for Holstein cattle. The prevalence of liver abscesses in the Central Plains region (CR) is greater than the Southwest region (SR) of the United States. The objective was to evaluate soil organic matter (SOM) microbiome of feedlot pens, associated LA microbiomes, and the impact of breed, location, and tylosin. Eighty-three commercial feedlot pens were sampled for SOM prior to harvesting and LA samples were collected from two commercial packing plants in Tolleson, AZ (SR) and Greely, CO (CR). Liver abscess samples were linked to SOM samples. Amplification and sequencing of the V4 variable region of the 16S rRNA gene was performed using the Illumina MiSeq. Bacterial phyla, genera, and diversity indices were reported for LA and SOM samples. Reported data is from a subset of five pens. The most prevalent genera identified in liver abscesses were Fusobacterium (64.2%) and Bacteroides (18.6%). Predominant SOM genera were Atopostipes (13%) and Clostridium (10%). Phylum SOM Shannon Weiner Index (SWI) was lower for the CR compared to the SR (0.034 vs. 0.174; P < 0.05), indicating a dominant bacterium in the CR. Atopostipes and Clostridium comprised 48% of the CR SOM, explaining the lower SWI. The SWI of SOM and liver abscesses of Holsteins are lower (P < 0.05) compared to beef counterparts. Additionally, Clostridium, Fusobacterium, and Atopostipes populations were greater (P < 0.05) in SOM of Holstein pens than beef pens. Genus richness was lower (P < 0.05) for both SOM and liver abscesses from pens of cattle fed tylosin vs. no tylosin. There is a negative correlation between SOM SWI and LA% (R2 = -0.49; P = 0.03) and LA severity (A+) (R2 = -0.44; P = 0.05). In conclusion, SOM may serve as a potential vector for liver abscesses.
PSIV-11 Effects of very low-dose antibiotics on gene expression profiles in ileal mucosa of weaned pigs infected with a pathogenic E. coli
Authors: Kim Kwangwook , Jang Sungbong , Liu Yanhong
Published: November 2020

Our previous studies have shown that supplementation of low-dose antibiotic growth promoter (AGP) exacerbated growth performance and systemic inflammation of weaned pigs infected with pathogenic Escherichia coli (E. coli). The objective of this experiment, which is extension of our previous report, was to investigate the effect of low-dose AGP on gene expression in ileal mucosa of weaned pigs experimentally infected with F18 E. coli. Thirty-four pigs (6.88 ± 1.03 kg BW) were individually housed in disease containment rooms and randomly allotted to one of three treatments (9 to 13 pigs/treatment). The three dietary treatments were control diet (control), and 2 additional diets supplemented with 0.5 or 50 mg/kg of AGP (carbadox), respectively. The experiment lasted 18 d [7 d before and 11 d after first inoculation (d 0)]. The F18 E. coli inoculum was orally provided to all pigs with the dose of 1010 cfu/3 mL for 3 consecutive days. Total RNA [4 to 6 pigs/treatment on d 5; 5 to 7 pigs/treatment on 11 post-inoculation (PI)] was extracted from ileal mucosa to analyze gene expression profiles by Batch-Tag-Seq. The modulated differential gene expression were defined by 1.5-fold difference and a cutoff of P < 0.05 using limma-voom package. All processed data were statistically analyzed and evaluated by PANTHER classification system to determine the biological process function of genes in these lists. Compared to control, supplementation of recommended-dose AGP down-regulated genes related to inflammatory responses on d 5 and 11 PI; whereas, feeding low-dose AGP up-regulated genes associated with negative regulation of metabolic process on d 5, but down-regulated the genes related to immune responses on d 11 PI. The present observations support adverse effects of low-dose AGP in our previous study, indicated by exacerbated the detrimental effects of E. coli infection on pigs’ growth rate, diarrhea and systemic inflammation.
PSIV-25 Effect of Various Handling Methods of Plasma and Serum Samples on Pregnancy Associated Glycoprotein Concentrations
Authors: Wesson Grace M , Fernandez Lohana , Poole Rebecca K , Franco Gessica A , Reese Sydney T , Pohler Ky G
Published: November 2020

Pregnancy associated glycoproteins (PAG) can be used as a biomarker for early pregnancy diagnosis, so accurate and consistent PAG detection is critical. The objective of this study was to determine if plasma and serum PAG concentrations were altered when centrifugation occurred at different times post-collection, when subjected to repeated freezing and thawing, and when monoclonal antibodies were kept in frequently or infrequently opened containers. Plasma (n = 4) and serum (n = 4) samples were collected from two open cows and two pregnant cows 28 days after artificial insemination. Pregnancy status was determined via transrectal ultrasonography. Plasma and serum samples were evenly separated and either centrifuged on the day of collection, or placed at 4°C and centrifuged the next day. An in-house PAG ELISA was performed on all samples before freezing (NOTHAW), after being frozen for one week (INTACT), after one freeze/thaw cycle (THAW1), two freeze/thaw cycles (THAW2), and three freeze/thaw cycles (THAW3). Data were analyzed using one-way ANOVA (GLM procedure, SAS 9.4). All samples from open cows were below the baseline of the assay. For pregnant cows, plasma samples had greater PAG concentrations than serum samples (11.84 vs 3.30 ± 0.66 ng/mL, respectively, P < 0.05). No differences were observed for day of centrifugation in both plasma and serum samples (P = 0.50 and P = 0.60, respectively) and in handling of monoclonal antibodies (P = 0.90). Freezing and thawing did not impact PAG concentrations in plasma samples (P = 0.19), but did alter serum concentrations (P = 0.01). Specifically, THAW1 (1.98 ng/mL) and THAW2 (1.42 ng/mL) serum PAG concentrations were lower compared to NOTHAW, THAW3, and INTACT samples (4.66, 4.85, and 3.57 ng/mL, respectively). Based on these data, plasma yields more consistent results than serum, even after several freeze-thaw cycles, and handling of monoclonal antibodies or time of centrifugation has no significant effect on measured PAG.
PSVII-20 Supplementing pHix-Up® to feedlot cattle consuming a corn-based finishing diet
Authors: Colombo Eduardo , Cooke Reinaldo F , Brandão Alice , Souza Rodrigo , Duclos Julie , Schubach Kelsey , Pohler Ky G
Published: November 2020

This experiment evaluated the inclusion of a rumen-buffering agent on performance and carcass traits of feedlot cattle. Angus-influenced steers and heifers (n = 64 animals/sex) were ranked by sex and body weight (BW) into 1 or 16 drylot pens. Pens were randomly assigned to receive a corn-based diet without (CON = 8 pens) or with the inclusion (SUPP = 8 pens) of pHix-Up® (magnesium-based product; Timab, Dinard, France) at 0.50% of diet dry matter. Both dietary treatments contained monensin Na (360 mg animal/daily) but did not contain tylosin. Two animals per pen were equipped with an intraruminal bolus (smaXtec Premium Bolus, smaXtec Animal Care GmbH, Graz, Austria) that recorded pH every 15 min. Cattle received treatments for 128 d until slaughter. No differences (P ≥ 0.22) between SUPP and CON cattle were noted for BW gain (0.93 vs. 0.97 kg/d, respectively), feed intake (7.51 vs. 7.78 kg/animal daily, respectively), or feed efficiency (0.121 vs. 0.125 kg/kg, respectively). Rumen pH was greater in SUPP vs. CON cattle during 33% of the time (e.g. 6 h of the day; mean pH of 5.87 vs. 5.80, respectively). No treatment differences were noted (P ≥ 0.12) for carcass merit. Incidence of cattle with liver abscess upon slaughter was greater in CON vs. SUPP (16.4 vs. 4.7%, respectively), including incidence of severe liver abscess (A+; 8.2 vs. 0.0%, respectively). These results indicate that pHix-Up® inclusion into a corn-based finishing diet improved rumen pH without benefiting cattle productive responses. It should be noted that average temperature-humidity index during this experiment was 78, which hindered feed intake and potential benefits of pHix-Up® to rumen pH and cattle performance. Nonetheless, pHix-Up® inclusion substantially reduced the incidence of liver abscess, resulting no severe diagnoses. Hence, additional research is warranted to further explore the benefits of pHix-Up® to feedlot cattle.
PSVII-3 Correlations between L-lactate concentrations obtained using a handheld lactate analyser and a lactate assay colorimetric kit in beef cattle transported by road
Authors: Meléndez Daniela M , Marti Sonia , Faucitano Luigi , Haley Derek B , Schwinghamer Timothy D , Nordi Wiolene M , Schwartzkopf-Genswein Karen S
Published: November 2020

Blood metabolites are used to assess a variety of animal conditions for veterinary diagnosis and research. Concentration of metabolites in blood can be measured using a commercially-available lab-based assay or in real-time using a handheld device developed to be more time- and cost-effective than the lab-based method. Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer (Lactate Scout, EFK Diagnostics, Barleben, Germany) and a lactate assay colorimetric kit (Lactate Assay Kit, Cell Biolabs Inc., San Diego, CA). Blood samples were collected by venipuncture from 96 steers (245 ± 35.7 kg BW) prior to (L1) and after 36 h, and prior to and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in colorimetric analysis. Pearson correlations were calculated to determine the relationship between the experimental methods for the quantification of L-lactate concentrations. The strengths and levels of statistical significance of the correlation varied over the observed time points, r = -0.03, P = 0.75 (L1) to r = 0.75, P = < 0.0001 (d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, P < 0.001). Based on the experimental results, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay for measuring L-lactate in transported cattle, due to variability across sampling time points and weak correlation with the traditional enzymatic method.
PSVII-7 Equations for estimating commercial meat cuts of water buffaloes finished in feedlot
Authors: Jacob Vanessa R M , Castilhos André M , Francisco Caroline L , Luz Patricia A C , Marques da Silva Daiane C , Jacaúna Amanna G , Oliveira Camila S , Paula Tania V , de Barros Felipe , Jorge Andre M
Published: November 2020

Equations for estimating commercial meat cuts of water buffaloes finished in feedlot (means of initial body weight and age of 314 ±117 kg and 13 ±1.2 months, respectively) using biometric measurements performed in vivo, carcass traits performed postmortem, and the set of both measurements were determined. Seventy-five non-castrated males (25 of each genetic group: Jafarabadi, Mediterranean and Murrah) were used and allocated in collective pens to receive feeding and water ad libitum for 240 days. Body weight (BW), hip height (HH), and ultrasound assessments (backfat thickness – BFT; Ribeye area – REA) were performed and tested as independent variables. After the slaughter, the non-carcass components of each animal were weighed to obtain the empty body weight (EBW). Hot carcass weight (HCW), cold carcass weight (CCW), carcass length (CL, cm), carcass depth (CD, cm), and carcass compactness index (CCI, kg/cm) were obtained and tested as independent variables. The meat cuts (Brisket, Flank plate, Rump, Rump cap, Striploin, and Tenderloin) were obtained from right half-carcass and weighed. The equations were determined using the stepwise regression method and Mallows’ Cp criterion, and processed by the REG procedure in SAS. The independent variables which were included differed among the meat cut equations which can use both independent variables obtained in vivo and postmortem, or only those obtained postmortem: Brisket, kg = 0.165-0.004×EBW+0.007×HCW+0.017×CCW (R2 =0.81, SE =0.27, Cp=1.81); Flank plate, kg = -2.643-0.013×HCW+0.028×CCI +2.681×CL-1,021×HH (R2 =0.68, SE =0.116, Cp=2.73); Rump, kg = -4.975-0.004×EBW-0.004×HCW+0.032×CCI+4.771×CL-1.662×CD-0.017×BFT (R2 =0.84, SE =0.18, Cp=6.17); Rump Cap, kg = -0.485-0.002×EBW+0.011×CCI -1.291×CL (R2 =0.78, SE =0.19, Cp=2.20); Striploin, kg = -0.088-0.004×EBW+0.020×HCW +0.019×CCI-2.949×CL (R2 =0.85, SE =0.35, Cp=3.97); Tenderloin, kg = -2.125 + 0.008×CCI +1.863×CL-1.697×CD+0.698×HH-0.014×BFT (R2 =0.84, SE =0.12, Cp=5.89). In conclusion, the equations for estimating commercial meat cuts present good prediction and can be used for different GG of water buffaloes. Supported by FAPESP (#2014/05473-7).
PSVII-2 Blood concentrations of leptin, glucose, insulin, cholesterol, triglycerides, and lipoproteins and their association with feedlot performance and efficiency measurements of water buffaloes
Authors: Oliveira Camila S , Francisco Caroline L , Castilhos André M , da Silva Fabiola Martinez , Correa Hugo L , Jacaúna Amanna G , de Barros Felipe , Paula Tania V , Jacob Vanessa R M , Jorge Andre M
Published: November 2020

This study evaluated the association between serum parameters and feedlot performance, and efficiency measures of water buffalo for meat production. Seventy-five animals of three different genetic groups (GG:Jafarabadi, Mediterranean, and Murrah) were used, with means of initial body weight (BW) and age of 314±117 kg and 13±1.2 months, respectively. Animal BW were recorded at the beginning and every 28 days until the final of the efficiency period (EP; 84 days). Animals of each GG were allocated in collective pens provided with artificial shade and automatic feeder system to determine individual daily intake of diet and water offered ad libitum. Average daily gain (ADG, kg/day), dry matter intake (DMI, in kg/day and %BW) were evaluated. Efficiency measures [feed conversion ratio (F:G), feed efficiency (G:F), residual feed intake (RFI), residual gain (RG), and residual intake and gain (RIG)] were calculated considering 84 days of experiment. Blood samples were collected from the jugular vein on days 0, 56, and 84, and were evaluated for serum levels of leptin, glucose, insulin, cholesterol, triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) by commercial kits. Animals were clinically healthy and with no skin lesion, intestinal and blood parasites, as well as no diarrhea, urogenital or muscular abnormalities. Data were analyzed using the CORR procedure with partial command in SAS. There are some correlations between serum parameters and feedlot performance and efficiency measurements (P ≤ 0.05) as well as there are tendencies (P ≤ 0.10; Table 1). Furthermore, leptin, TRI, and lipoproteins serum levels are the parameters most associated with some performance and efficiency variables. In conclusion, although weak, the correlations suggest that there are alterations of serum parameters according to performance and efficiency and these parameters can be used as tools to assist in decision making within the buffalo production system. Supported by FAPESP (#2014/05473-7).
PSVII-6 Effects of 25-hydroxycholecalciferol supplementation on gene expression of feedlot cattle
Authors: Martins Tainá E , Acedo Tiago S , Gouvea Vinicius N , Vasconcellos Guilherme S , Arrigoni Mário B , Martins Cyntia L , Millen Danilo D , Pai Maeli D , Perdigão Alexandre , Melo Gabriel F , Rizzieri Ramon A , Rosolen Leonardo M , Costa Carolina , Sartor Ana Bárbara
Published: November 2020

The objective was to evaluate the effects of 25-hydroxycholecalciferol (HyD®, DSM Produtos Nutricionais Brasil S.A.) supplementation in expression of genes related to anabolism and catabolism of feedlot cattle. A total of 120 Nellore bulls (IBW = 370±20 kg) were distributed in 24 fully roofed-pens with concrete floor (5 animals/pen) in a randomized complete block design (8 pens/treatment). The treatments were T1 (control): no supplementation of HyD®; T2: HyD® supplementation at 1 mg/animal/day; T3: HyD® supplementation at 3 mg/animal/day. Basal diet was formulated to meet requirements of finishing bulls, considering an ADG of 1.6 kg/day (Level 2 Nutrition System, Fox et al., 2004). Adaptation to diets followed a step-up scheme for 14 days, with concentrate inclusion being gradually increased from 76 to 91% DM. Finishing diets were offered from 15th to 100th day of feedlot period and animals fed twice a day (0800 and 1500), with bunks managed for a maximum of 5% orts. After slaughter, muscle samples were collected for quantitative evaluation of gene expression using RT-qPCR method, considering the following genes: SOD1 (antioxidant marker), IGF1, IGF2 and MTOR (anabolism), FOXO1, MURF1, Atrogin-1 and MSTN (catabolism). Statistical analysis was performed using PROC MIXED of SAS® and means compared by Tukey test at 5% probability. No significant differences among treatments were observed for SOD1, FOXO1, MURF1 and Atrogin-1 expression (P >0.05). However, tendencies (0.05 > P ≤0.10) could be observed for IGF1, IGF2, MTOR and MSTN expression in animals receiving HyD®, regardless of dosage. These results indicate a positive effect of HyD® on muscular anabolism and protein synthesis on feedlot finishing cattle. Moreover, the greater expression on MSTN suggest a higher protein turnover and muscular growth regulation. In conclusion, HyD® supplementation increased expression of genes correlated to muscular growth and protein synthesis, being a viable technology for beef cattle finished in feedlot systems.
PSVII-9 Evaluating Performance Trends of the Four Most Represented Breeds of Bulls in the Utah Beef Improvement Performance Test
Authors: Garcia Matthew D , Kunzler Madelyn , Chapman Kim
Published: November 2020

This study evaluated changes in performance of bulls enrolled in the Utah Beef Improvement Association’s (UBIA) annual performance tests conducted in accordance with central bull test standards established by the Beef Improvement Federation. Breeds with over 500 bulls enrolled over the period of 2004 to 2018 were included in the evaluation. The breeds with greater than 500 animals evaluated included the Angus, Red Angus, Hereford and Simmental Breeds. Traits evaluated included birth weight, initial weight, a 205-day adjusted weaning weight, final test weight, average daily gain, adjusted yearling weight, and weight per day of age. The mixed model procedure of SAS and interval regression analyses were utilized to evaluate the yearly rate of change for each breed and to determine if that rate of change was significantly different (P < 0.05) between the evaluated breeds. When evaluating all breeds represented across years, significant differences (P < 0.05) were observed for rate of change when evaluating birth weight and 205-day adjusted weaning weight. However, no differences were observed between breeds with more than 500 animals represented. Significant differences (P < 0.05) for rate of change were observed when comparing the top four represented breeds, for average daily gain, initial test weight, final test weight, adjusted yearling weight and weight per day of age. Specifically, a declining trend was noted for all breeds when evaluation all significant traits. When evaluating the previously described traits, the Angus breed was significantly different in its rate of decline when compared to the Red Angus and Hereford breeds. The lone increasing trend was observed in the Hereford breed which had a positive increasing trend for initial test weight which was significantly different (P < 0.05) from both the Angus and Red Angus breeds but not significantly different from the Simmental breed.
PSVII-21 The impact of cell culture and copper dose on copper trafficking genes in bovine liver
Authors: Tillquist Nicole M , Thorndyke Meghan P , Thomas Tyler A , Coleman Stephen J , Engle Terry E
Published: November 2020

The objective of the current experiment was to investigate the influence of Cu dose on the relative abundance of Cu trafficking genes in cultured bovine hepatocytes. A liver sample was obtained immediately post-mortem from one healthy Angus steer. Hepatocytes were isolated, counted, and seeded at equal density into 15 separate wells, and incubated for 1 hour in culture media containing: 0.0, 0.10, 1.0, 10.0, or 100 mg Cu/L (3 replicates per Cu dose). Following incubation, cells were collected and total RNA was isolated. Quantitative RT-PCR was used to determine the abundance of transcripts for proteins involved in Cu homeostasis. The identified targets were: ALDH2, APOA1, ATOX1, ATP7A, ATP7B, BHMT, BLVRB, CA2, CCS, COX17, CTR1, ELN, GAPDH, GLUD1, GSS, LOXL1, PDIA3, SOD1, SOD3. β-Actin (ACTB) served as the endogenous control. Significant linear responses existed for ALDH2 (P < 0.001), ATOX1 (P < 0.01), PDIA3 (P < 0.05). As Cu dose increased, the relative abundance of ALDH2 increased, and ATOX1 and PDIA3 decreased. Significant quadratic responses existed for ATP7B (P < 0.001), COX17 (P < 0.05), and SOD1 (P < 0.05). The relative abundance of COX17 was lesser at 0.1 and 1.0 mg Cu/L when compared to 0.0, 10, and 100 mg Cu/L. Transcript abundance for ATP7B and SOD1 was lower at 0, 1, and 100 mg Cu/L when compared to 0.1 and 10 mg Cu/L. These data indicate that certain transcripts are differentially expressed in cultured bovine hepatocytes in response to increasing Cu dose.
PSVI-30 Requirements of the sulfur amino acids in growing Labrador Retrievers using the indicator amino acid oxidation technique
Authors: Weil Jordan T , Varney Jessica L , Fowler Jason W , Coon Craig N
Published: November 2020

Over time, the need to update amino acid (AA) requirements for canines becomes increasingly important to ensure animals are healthy and free of nutritional disease. Each of the AA plays a crucial role in the metabolism and health of mammals. Of the essential AA, the total sulfur amino acids (TSAA), namely methionine (Met) and cysteine (Cys), are involved in metabolic functions such as protein metabolism, intestinal health, and urinary function. Additionally, Met can function as a precursor in the requirement of Cys. The purpose of this experiment was to use the indicator amino acid oxidation technique to determine amino acid requirements on growing Labrador Retrievers. A total of 12 dogs were subjected to 12 diets with varying levels of Met and Cys, ranging from deficient to excess. Diets were formulated to 1.6x NRC values for all indispensable amino acids. The control diet was fed for two days, followed by a day in which the test diet was fed, a tracer amino acid was supplied, and breath samples were collected. On test day, a priming dose of L-[1-13C]phenylalanine (Cambridge Isotope Laboratories, Inc.) based on the subject’s body weight was first supplied, followed by [1-13C]Phe doses every thirty minutes, spanning a four hour period. A respiration mask was placed on each subject every thirty minutes (Oxymax, Columbus Instruments), 13CO2 was collected, and enrichment was determined by isotope ratio mass spectrometry (IRMS). Results for IRMS were converted to atom percent excess (APE) and analyzed using a broken line model (JMP® Pro 15). Results showed that the Met and TSAA mean and population requirements were 0.78 ± 0.16 and 1.53 ± 0.21 g/1000kcal (mean ± 2SD), respectively. Knowledge gained from these studies is necessary as the petfood industry aims to deliver accurate diet formulations to the expansive canine population.
PSVIII-28 Intercropping corn with field peas or forage soybeans for use as silage
Authors: Rodehorst Wayde J , Ostlie Mike H , Moore Rebecca L , Neville Bryan W
Published: November 2020

The objective of this study was to evaluate forage production and compare nutrient content of intercropped corn/legume silages. Forage production was determined by harvesting 3 m by 7.7 m plots (n = 4). Corn was seeded at 79,000 seeds/ha and intercropped with 408,000, 538,000, or 815,000 seeds/ha of field peas; or corn intercropped with 272,000, 359,000, or 544,000 seeds/ha of forage soybean. All forages were harvested on a common date. Subsamples of forage were utilized in a laboratory scale ensiling study. Forage samples were stored in vacuum bags for 28 d prior to determination of pH, DM, and nutrient analysis. Intercropping field peas with corn decreased forage production compared to corn alone (P = 0.007). The lower field pea seeding rate resulted in greater forage production (P = 0.002) than either of the higher seeding rates which did not differ (P = 0.65). Intercropping forage soybean with corn increased (P < 0.001) forage production over corn alone. Intercropping corn with field peas increased silage CP (5.8 vs. 4.5 ± 0.13; P < 0.001) compared to corn alone. Including field peas at the lower and higher rate increased (P < 0.001) silage CP compared to the mid-rate and corn alone. Inclusion of soybeans increased silage CP over corn alone (5.9 vs 4.5 ± 0.16; P < 0.001). The middle seeding rate of forage soybean resulted in the greatest silage CP (P < 0.001). All rates of field pea inclusion resulted in decreased (P = 0.03) silage pH (3.8 vs 3.9 ± 0.04) compared to corn alone. Intercropping soybeans resulted did not affect (P = 0.81) silage pH (3.9 ± 0.04) compared to corn alone. Lodging contributed to the decreased yield of intercropped corn and field peas. Further research is needed prior broader recommendation related to intercropping corn and legumes for silage.
PSVIII-34 Late-Breaking Abstract: Additivity of digestible amino acids in hatchery byproducts fed to nursery pigs
Authors: Kim Beob G , Sung Jung Yeol
Published: November 2020

The objectives were to determine standardized ileal digestibility (SID) of amino acids (AA) in hatchery byproducts fed to nursery pigs and to test the additivity of ileal digestible AA concentrations in hatchery byproducts. Ten barrows (initial body weight = 11.3 ± 1.3 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in pens. Four diets were prepared to contain 30% of infertile eggs, unhatched eggs, culled chicks, or a mixture (20% dried infertile eggs + 20% dried unhatched eggs + 60% dried culled chicks) as the sole source of nitrogen. A nitrogen-free diet was additionally prepared to measure basal endogenous losses of AA. All experimental diet contained 0.5% chromic oxide as an indigestible index. Ten pigs were assigned to a replicated 5 × 4 Latin square design with 5 diets and 4 periods. Each period consisted of a 5-d adaptation period and a 2-d collection period. The hypothesis was that the determined digestible AA concentrations of the mixture are not different from the calculated values based on digestible AA in the individual ingredients. A t-test was used to determine the additivity of digestible AA concentrations in individual ingredients. Infertile eggs had greater (P < 0.05) SID of all indispensable AA except for lysine and methionine compared with culled chicks. The SID of all indispensable AA except for tryptophan was not different between culled chicks and the mixture. The calculated apparent and standardized ileal digestible AA in the mixture were not different from the determined values, but calculated ileal digestible tryptophan in the mixture was less (P < 0.05) than the measured value. In conclusion, ileal digestibility of most indispensable AA in infertile eggs is greater than that in culled chicks and ileal digestible AA values are additive in the mixture except for tryptophan.
PSVIII-37 Late-Breaking Abstract: Effects of feeding a vitamin and mineral supplement and (or) an energy supplement on the abundance of SLC7A5 transporter in beef heifer placentomes at d 83 of gestation
Authors: Menezes Ana Clara B , McCarthy Kacie L , Kassetas Cierrah J , Baumgaertner Friederike , Kirsch James D , Dorsam Sheri T , Neville Tammi L , Ward Alison K , Borowicz Pawel P , Reynolds Lawrence P , Sedivec Kevin K , Forcherio J Chris , Scott Ronald , Caton Joel , Dahlen Carl R
Published: November 2020

Objectives were to evaluate effects of vitamin and mineral (VTM) and (or) energy (NRG) supplements on the abundance of the neutral amino acid (AA) transporter SLC7A5 in heifer placentomes at d 83 of gestation. Thirty-five crossbred Angus heifers (Body weight = 359.5 ± 7.1 kg) were randomly assigned to one of four treatments (n = 8 or 9/group) in a 2 × 2 factorial arrangement with main effects of vitamin and mineral (NoVTM or VTM) and energy (NoNRG or NRG) supplements. The VTM treatment was initiated 71 to 148 d before artificial insemination (AI). The NRG treatment was initiated at AI. Heifers were ovariohysterectomized at d 83 of gestation. Placentome cross sections (5 μm) were mounted onto glass slides, deparaffinized, and stained for SLC7A5. Fluorescent images of tissue areas including fetal membranes, caruncles (CAR), endometrial epithelium (EP), superficial glands (SG), deep glands (DG), and myometrium were obtained and analyzed for relative intensity of fluorescence within the areas of interest as an indicator of transporter abundance. The abundance of SLC7A5 was influenced by a VTM × NRG interaction (P = 0.05) in DG, with NoVTM-NRG being greater than heifers receiving VTM. Abundance of SLC7A5 was not influenced by NRG (P > 0.12) in any structures evaluated. In EP and SG abundance of SLC7A5 was less (P ≤ 0.05) in VTM than NoVTM heifers and tended to be less in CAR (P = 0.07). Our previous work revealed no differences in maternal serum AA concentrations, but VTM heifers had greater AA concentrations in allantoic fluid than NoVTM. We hypothesize that transporter efficiency may be reduced in NoVTM heifers, with greater transporter abundance being a mechanism of compensation to supply AA to the fetus. Together, these data help to further elucidate mechanisms of AA uptake and balance between the maternal and fetal compartments.
PSX-33 Late-Breaking Abstract: Productive longevity of Holstein cows and causes of their replacement
Authors: Abylgazinova Aizhan , Shamshidin Alzhan , Yesseyeva Gainiya , Serikbaeva Aliya , Ayupova Almagul , Tilepova Assel
Published: November 2020

One of the main ways to increase the milk production of cows and extend the terms of their use is targeted breeding work. Currently, the long-term economic use of cows on average in Kazakhstan is 3.2 lactations. Thus, cows do not live up to 4–6 lactation, when the highest productivity is manifested and the costs of raising heifers, pregnant heifers and keeping productive animals pay off. (Komarov V.Y, 2019)In this regard, we studied the duration of the economic use of cows, taking into account their lifelong productivity. Objective and research technique. The purpose is to study the duration of the productive use of cows and the reasons for their replacement at Aina Dairy Farm LLP. The subjects of this research were 631 Holstein cows. An analysis of the reasons for culling cows at Aina Dairy Farm LLP showed that up to 25% of cows were replaced with udder diseases, up to 50% with hoof and limb diseases, up to 15% with reproductive dysfunctions, and only 10% in terms of productivity and age. Cows who left during the first lactation and didn’t manage to recoup the costs of growing, which caused a loss to farms, make up 30% of all rejected. Cows who left on the second lactation and only recouped their own costs and didn’t bring profit, make up 25%. (Serikbaeva A.K.,Tilepova A.K.,2020) To achieve this goal, the following tasks were solved: identifying the reasons for the cows to be replaced, determining the replacement age, calculating productive longevity and the dynamics of its change in calving. Analysis of productive longevity showed that a large number of cows are replaced from the herd during the first and second lactation, mainly due to health problems associated with metabolism. The main cause of morbidity is feeding, milking and milking conditions.
PSV-29 Influence of Akaushi Genetics on Color and Sensory Attributes of Beef from Grain and Grass-finishing systems
Authors: de Faria Maciel Isabella Cristina , Schweihofer Jeannine P , Fenton Jenifer I , Hodbod Jennifer , McKendree Melissa , Cassida Kim , Rowntree Jason E
Published: November 2020

The effects of beef genetics and finishing system on color and sensory attributes of longissimus dorsi steaks were evaluated. Steaks were from Red Angus (RA, n = 30) and RA x Akaushi (AK, n = 30) fed with a mixed-species pasture forage (GRASS) or a total mixed feedlot ration (GRAIN). Animals were slaughtered at 18 (GRAIN) and 26 (GRASS) mo. Rib sections (11th and 13th) were collected 48 h postmortem from the left side of each carcass, cut into two 2.54 cm-thick steaks, vacuum packaged, and aged 14 d. One steak was frozen until water holding capacity and Warner Bratzler shear force (WBSF) analysis. The other fresh steak was evaluated for instrumental color and cooked for consumer panelists (n = 105) to evaluate flavor, texture, juiciness, and overall acceptability using a 9-point hedonic scale (1 = dislike extremely and 9 = like extremely). There was no difference between beef genetics. GRASS had higher chroma (more color saturation) and a* values (redder) compared to GRAIN treatment. There was genetics x diet interaction for hue angle and L* values, where RA had greater hue angle than AK in GRAIN (P = 0.01) and AK had greater L* (lighter, P < 0.01) than RA in GRASS. The GRASS-finishing reduced beef steaks thawing (-20 %) and cooking (-12.8 %) loss compared to GRAIN-finishing (P < 0.05). There were no differences (P > 0.05) in WBSF between finishing systems. Steaks from GRAIN received the greatest ratings (P < 0.01) for flavor, texture, juiciness, and overall acceptability compared to GRASS. Results indicate that the color attributes were influenced by finishing system. Furthermore, beef finishing system had a marked impact on steaks’ sensory attributes and consumer acceptability. The favorable results for texture and juiciness in GRAIN, which likely impacted overall acceptability, may be related to high marbling.
PSIII-17 Program Chair Poster Pick: A study on the effect of dihydroquercetin added into a diet of growing pigs on meat quality
Authors: Semenova Anastasiia A , Nasonova Victoria V , Kuznetsova Tatiana G , Tunieva Elena K , Bogolyubova Nadezhda V , Nekrasov Roman V
Published: November 2020

Dihydroquercetin (DHQ; taxifolin) and quercetin are interesting for pig breeding as adaptogens positively affecting the antioxidant status of animals. The aim of the study was a comparative quality analysis of pork from the control and experimental pig groups (hybrid young barrows F2 (LWxL)xD with livestock weight of 35.0±1.0 kg at the beginning of the experiment; 18 animals) exposed to the technological stress (rearrangement) and received 0 and 32 mg DHQ per 1 kg feed, respectively, throughout the feeding period up to livestock weight of 115.0±1.5 kg. Pork (m. L.dorsi) was analyzed 24 hours after slaughter. In the control and experimental groups, the loin eye area was 61.02±2.54 and 61.51±3.85 сm2 (р=0.83); the content of moisture was 68.9±2.2 and 70.9±0.6% (P = 0.39), fat 7.0±2.6 and 4.3±0.8% (P = 0.34), protein 22.7±0.3 and 23.3±0.6% (P = 0.39); рН 24 was 5.39±0.05 and 5.46±0.09 (P = 0.51); the moisture holding capacity was 65.97±1.96 and 68.36±2.55% (P = 0.47); color: lightness 56.56±2.22 and 60.58±3.16 (P = 0.31), redness 3.34±1.06 and 5.54±2.54 (P = 0.44), yellowness 9.42±1.43 and 10.79±1.64 (P = 0.55), respectively. The intensity of meat odor (a.u. х 107) was 28.29±7.79 and 19.46±2.23 (P = 0.29) in raw meat, 123.61±14.49 and 113.06±4.58 (P = 0.50) in cooked meat, respectively. The obtained data are in agreement with the works by Kremer B.T., 1999; Zou Y., 2016; Ivanova S.G., 2019 and others, who demonstrated an insignificant or ambiguous contribution of adaptogens into meat quality. Therefore, despite the positive trend of the DHQ effect on pork quality, statistically significant differences between two groups were not established. The work was supported by the grant No. 19-16-00068 of the Russian Science Foundation.
PSIII-29 Feeding arsenic-containing rice bran to growing pigs: arsenic distribution in major tissues
Authors: Liao Shengfa F , Hasan M Shamimul , Yang Zhongyue , Stevens Andrew W , Brett James , Peng Zhaohua
Published: November 2020

Previous research suggested that arsenic (As) is required by pigs, while inorganic As is a non-threshold carcinogen to humans. This research was conducted to study the As tissue distribution in pigs fed As-containing rice bran (833 ppb). Twenty individually penned gilts (26.3 kg) were randomly assigned to 3 dietary treatments (n = 6 or 7) with Diets I, II, and III containing 0, 306, and 612 ppb As, respectively. Pigs were fed ad libitum for 6 weeks, and the blood and hair samples were collected immediately before and after. Pigs were harvested at the end of the 6-weeks, and the liver, kidney, and muscle samples were collected. The total As contents in all samples were determined by a commercial laboratory. Data were analyzed with ANOVA, Tobit, or ordinary least squares regression model where appropriate. No pig displayed any poisoning signs throughout the trial. The hair As concentrations of Diets II and III pigs were both higher (P < 0.003) than Diet I pigs, and the hair As concentration of Diet III pigs was higher (P = 0.007) than Diet II pigs. The blood As concentrations were all below the detection limit. In kidney and liver, the As concentrations in Diets II and III pigs were higher (P = 0.01) and numerically higher (P < 0.30), respectively, than Diet I pigs, and there was no difference (P > 0.10) between Diets II and III pigs. In skeletal muscle, the As concentrations were all below 10 ppb. These As tissue distribution data indicate that the absorbed As was rapidly cleared from the blood with some retained in various organs. While pigs’ hair retained the highest level of As, the retention is much lower in the liver and kidney. The muscle As data suggest that the pork produced from the pigs fed a typical As-containing rice bran is safe for human consumption.