bluuebunny/crossref_metadata_2025 huggingface dataset:
Precision medicine applied to metastatic colorectal cancer using tumor-derived organoids and in-vitro sensitivity testing: a phase 2, single-center, open-label, and non-comparative study
Authors: Jensen Lars Henrik , Rogatto Silvia Regina , Lindebjerg Jan , Havelund Birgitte , Abildgaard Cecilie , do Canto Luisa Matos , Vagn-Hansen Chris , Dam Claus , Rafaelsen Søren , Hansen Torben Frøstrup
Published: May 2023

Background

Patients with colorectal metastatic disease have a poor prognosis, limited therapeutic options, and frequent development of resistance. Strategies based on tumor-derived organoids are a powerful tool to assess drug sensitivity at an individual level and to suggest new treatment options or re-challenge. Here, we evaluated the method’s feasibility and clinical outcome as applied to patients with no satisfactory treatment options.

Methods

In this phase 2, single-center, open-label, non-comparative study (ClinicalTrials.gov, register NCT03251612), we enrolled 90 patients with metastatic colorectal cancer following progression on or after standard therapy. Participants were 18 years or older with an Eastern Cooperative Oncology Group performance status of 0–2, adequate organ function, and metastasis available for biopsy. Biopsies from the metastatic site were cultured using organoids model. Sensitivity testing was performed with a panel of drugs with proven activity in phase II or III trials. At the discretion of the investigator considering toxicity, the drug with the highest relative activity was offered. The primary endpoint was the proportion of patients alive without disease progression at two months per local assessment.

Results

Biopsies available from 82 to 90 patients were processed for cell culture, of which 44 successfully generated organoids with at least one treatment suggested. The precision cohort of 34 patients started treatment and the primary endpoint, progression-free survival (PFS) at two months was met in 17 patients (50%, 95% CI 32–68), exceeding the pre-defined level (14 of 45; 31%). The median PFS was 67 days (95% CI 51–108), and the median overall survival was 189 days (95% CI 103–277).

Conclusions

Patient-derived organoids and in-vitro sensitivity testing were feasible in a cohort of metastatic colorectal cancer. The primary endpoint was met, as half of the patients were without progression at two months. Cancer patients may benefit from functional testing using tumor-derived organoids.

Trial registration

ClinicalTrials.gov, register NCT03251612.
Tumor microenvironmental modification by the current target therapy for head and neck squamous cell carcinoma
Authors: Okuyama Kohei , Naruse Tomofumi , Yanamoto Souichi
Published: May 2023

Current clinical and observational evidence supports the EXTREME regimen as one of the standards of care for patients with recurrent or metastatic head and neck squamous cell carcinoma (HNSCC) followed by the administration of immune checkpoint inhibitors (ICIs). In addition to the inhibition of the epidermal growth factor receptor (EGFR) pathway, cetuximab-mediated EGFR blockade has been shown to modulate tumor microenvironment (TME) characteristics, such as antibody-dependent cellular cytotoxicity (ADCC) activity, cytotoxic T-lymphocyte (CTL) infiltration into the tumor, anti-angiogenesis activity, and cytokine secretion via associated natural killer (NK) cells, etc.. On the other hand, there are reports that nivolumab affects the TME via Programmed cell death 1 (PD-1) inhibition, Interleukin-10 upregulation via T-cells, myeloid-derived suppressor cell-mediated immune escape induction, and tumor vessel perfusion by promoting CD8 + T-cell accumulation and Interferon-γ production in treatment-sensitive tumor cells. Actually, nivolumab administration can give T cells in the TME both immune superiority and inferiority. HNSCC treatment using cetuximab increases the frequency of FoxP3 + intratumoral effector regulatory T cells (Tregs) expressing CTL associated antigen (CTLA)-4, and targeting CTLA-4 + Tregs using ipilimumab restores the cytolytic function of NK cells, which mediate ADCC activity. Treg-mediated immune suppression also contributes to clinical response to cetuximab treatment, suggesting the possibility of the addition of ipilimumab or the use of other Treg ablation strategies to promote antitumor immunity. Moreover, also in hyper progression disease (HPD), intratumoral frequency of FoxP3 + effector Tregs expressing CTLA-4 is increased. Therefore, combination treatment with cetuximab plus anti-CTLA-4 antibody ipilimumab for HNSCC and this combination therapy after nivolumab administration for HPD may be expected to result in a higher tumor-control response. Based on the above evidence, we here suggest the efficacy of using these therapeutic strategies for patients with local-advanced, recurrent, and metastatic HNSCC and patients who do not respond well to nivolumab administration.
Intra-and inter-observer variability of point of care ultrasound measurements to evaluate hemodynamic parameters in healthy volunteers
Authors: Blanca Deborah , Schwarz Esther C. , Olgers Tycho Joan , ter Avest Ewoud , Azizi Nasim , Bouma Hjalmar R. , Ter Maaten Jan Cornelis
Published: May 2023

Background

Point-of-care ultrasound (POCUS) is a valuable tool for assessing the hemodynamic status of acute patients. Even though POCUS often uses a qualitative approach, quantitative measurements have potential advantages in evaluating hemodynamic status. Several quantitative ultrasound parameters can be used to assess the hemodynamic status and cardiac function. However, only limited data on the feasibility and reliability of the quantitative hemodynamic measurements in the point-of-care setting are available. This study investigated the intra- and inter-observer variability of PoCUS measurements of quantitative hemodynamic parameters in healthy volunteers.

Methods

In this prospective observational study, three sonographers performed three repeated measurements of eight different hemodynamic parameters in healthy subjects. An expert panel of two experienced sonographers evaluated the images’ quality. The repeatability (intra-observer variability) was determined by calculating the coefficient of variation (CV) between the separate measurements for each observer. The reproducibility (inter-observer variability) was assessed by determining the intra-class correlation coefficient (ICC).

Results

32 subjects were included in this study, on whom, in total, 1502 images were obtained for analysis. All parameters were in a normal physiological range. Stroke volume (SV), cardiac output (CO), and inferior vena cava diameter (IVC-D) showed high repeatability (CV under 10%) and substantial reproducibility (ICC 0.61–0.80). The other parameters had only moderate repeatability and reproducibility.

Conclusions

We demonstrated good inter-observer reproducibility and good intra-observer repeatability for CO, SV and IVC-D taken in healthy subjects by emergency care physicians.
A systematic literature review of indicators measuring food security
Authors: Manikas Ioannis , Ali Beshir M. , Sundarakani Balan
Published: May 2023

Measurement is critical for assessing and monitoring food security. Yet, it is difficult to comprehend which food security dimensions, components, and levels the numerous available indicators reflect. We thus conducted a systematic literature review to analyse the scientific evidence on these indicators to comprehend the food security dimensions and components covered, intended purpose, level of analysis, data requirements, and recent developments and concepts applied in food security measurement. Data analysis of 78 articles shows that the household-level calorie adequacy indicator is the most frequently used (22%) as a sole measure of food security. The dietary diversity-based (44%) and experience-based (40%) indicators also find frequent use. The food utilisation (13%) and stability (18%) dimensions were seldom captured when measuring food security, and only three of the retrieved publications measured food security by considering all the four food security dimensions. The majority of the studies that applied calorie adequacy and dietary diversity-based indicators employed secondary data whereas most of the studies that applied experience-based indicators employed primary data, suggesting the convenience of collecting data for experience-based indicators than dietary-based indicators. We confirm that the estimation of complementary food security indicators consistently over time can help capture the different food security dimensions and components, and experience-based indicators are more suitable for rapid food security assessments. We suggest practitioners to integrate food consumption and anthropometry data in regular household living standard surveys for more comprehensive food security analysis. The results of this study can be used by food security stakeholders such as governments, practitioners and academics for briefs, teaching, as well as policy-related interventions and evaluations.
What sets cooperative farmers apart from non-cooperative farmers? A transaction cost economics analysis of coffee farmers in Mexico
Authors: Trejo-Pech Carlos Omar , Servín-Juárez Roselia , Reyes-Duarte Álvaro
Published: May 2023

This study investigates what factors relate to the coffee farmer’s cooperative affiliation decision and whether this decision impacts the farmer’s cash holdings. First, we propose a cooperative affiliation model based on transaction cost economics theory. There is a lack of consensus in the literature on what factors explain the farmer’s cooperative affiliation decision in the coffee sector. Overall, we find that the more specialized coffee farmers are, the more likely they will become cooperative affiliates. This is consistent with transaction cost economics predicting that cooperatives are business structures that can reduce transaction costs and safeguard specialized assets from opportunistic behavior. Specifically, logit regression models suggest that shade-grown coffee plantations, off-farm income, coffee farming experience, low-level market competition, farmland size, altitude, and private farmland are statistically related to the farmer’s decision to affiliate with cooperatives. Results on farmland size and shade-grown coffee plantations can be particularly relevant for scholars, policymakers, cooperative leaders, and extension professionals in the region. Second, based on the affiliation model, we employ propensity score matching to evaluate the impact of the farmer’s cooperative affiliation decision on cash holdings, particularly on cash shortness. It is often claimed that farmers do not affiliate with cooperatives because these organizations cannot pay them in full at harvest and coffee collection time. It is believed that cooperatives’ inability to pay farmers early increases the likelihood of farmers’ cash shortness and their need for additional financing to operate or cover household needs. However, this study finds no evidence that the affiliation decision is related to the likelihood of the farmer experiencing cash shortness around harvesting and selling time.
Changes in color and iron ions of commercial iron gall inks after artificial aging
Authors: Xu Zhi , Wang Qiwen , Fan Huiming
Published: May 2023

Iron gall inks consist of vitriols (sulphates of certain metals), gall nut extracts; and gum Arabic: after exposure to oxygen, dark-colored compounds of the inks are formed. As the complexity of the composition of iron gall inks renders documents susceptible to environmental influences, this causes the handwriting thereon to fade. These add technical difficulties to the protection of iron gall inks. Therefore, it is particularly important to understand the changes in the inks during the aging process. For this reason, iron gall ink-stained paper specimens were subject to an intense analytical program to investigate their chemical and physical modifications after aging (temperature/humidity, temperature, and ultraviolet light aging), commercial iron gall ink was used for this experiment, making the study more applicable. The changes of iron gall inks were evaluated using color variation, color density, absorbance, and X-ray photoelectron spectroscopy (XPS). All results indicate that the temperature, humidity, and UV are harmful to the inks in both physical and chemical terms. Physical damage is mainly the aging of the ink color lightening, color density decreases, of which the color of the samples treated with damp heat for 30 days undergoes the greatest change. The chemical change is represented by the ratio of the concentration of iron ions in different valence states, the amount of Fe3+ in the untreated inks is much greater than that of Fe2+, and the amount of Fe2+ exceeds that of Fe3+ after exposure to different methods of aging. Experiments show that UV light causes the most severe damage to handwriting. The main manifestation thereof is color-fade and the paper surface ink part of the iron ion content changes, with the increase of aging time, the Fe2+ content gradually increases. This experimental study of the changes produced by iron gall inks during aging can provide better technical support for the protection of the ink handwriting.
Home-based tDCS for apathy in Alzheimer’s disease: a protocol for a randomized double-blinded controlled pilot study
Authors: Teixeira Antonio L. , Martins Laís Bhering , Cordeiro Thiago Macedo e , Jose Lijin , Suchting Robert , Holmes Holly M. , Acierno Ron , Ahn Hyochol
Published: May 2023

Background

Apathy is among the most common behavioral symptoms in dementia and is consistently associated with negative outcomes in Alzheimer’s disease (AD). Despite its prevalence and clinical relevance, available pharmacological and non-pharmacological strategies to treat apathy in AD have been marked, respectively, by potentially severe side effects and/or limited efficacy. Transcranial direct current stimulation (tDCS) is a relatively novel non-pharmacological method of neuromodulation with promising results. Compared to previous tDCS formats, recent technological advances have increased the portability of tDCS, which creates the potential for caregiver-administered, home use. Our study aims to evaluate the feasibility, safety, and efficacy of home-based tDCS for the treatment of apathy in AD.

Methods/design

This is an experimenter- and participant-blinded, randomized, sham-controlled, parallel-group (1:1 for two groups) pilot clinical trial, involving 40 subjects with AD. After a brief training, caregivers will administer tDCS for participants at home under remote televideo supervision by research staff to ensure the use of proper technique. Participants will be assessed at baseline, during treatment (week 2, week 4, and week 6), and 6 weeks post-treatment. Dependent measures will cover cognitive performance, apathy, and other behavioral symptoms. Data about side effects and acceptability will also be collected.

Discussion

Our study will address apathy, an overlooked clinical problem in AD. Our findings will advance the field of non-pharmacological strategies for neuropsychiatric symptoms, presenting a great potential for clinical translation.

Trial registration

ClinicalTrials.gov, NCT04855643.
Evaluation of short-term outcomes of neoadjuvant chemotherapy followed by radical cystectomy in muscle-invasive bladder cancer: a single Egyptian institution experience
Authors: Abdelrahman Ibrahim , Aboulkassem Hatem , Elazab Abdallah , Abdallah Ahmed Younis , Ismail Yahia , Taher Mohammad
Published: May 2023

Background and aim

Neoadjuvant chemotherapy (NAC) before radical cystectomy (RC) became the standard of care for muscle-invasive bladder cancer (MIBC) in the last few years. We aimed to evaluate the radiological, pathological responses to NAC, and the 30-day surgical outcomes after RC in MIBC.

Patients and methods

A retrospective cohort study involving adult patients with localized urothelial MIBC who received NAC followed by RC at the National Cancer Institute of Egypt (NCI-E) for 2 years (2017 and 2018). Out of 235 MIBC cases, we recognized 72 patients (30%) who fitted the eligibility criteria.

Results

A cohort of 72 patients with a median age of 60.5 years (range 34–87). Hydronephrosis, gross extravesical extension (cT3b), and radiologically negative nodes (cN0) were depicted initially in 45.8, 52.8, and 83.3% of patients, respectively. Gemcitabine and cisplatin (GC) was the rampant NAC employed in 95.8%. Radiological evaluation post NAC using RECIST v1.1 revealed a response rate (RR) of 65.3% in bladder tumor and progressive disease in the former and lymph nodes encountered in 19.4 and 13.9%, respectively. The median time from the end of NAC to surgery was 8.1 weeks (range 4–15). Open RC and ileal conduit were the most common types of surgery and urinary diversion, respectively. Pathological down-staging was encountered in 31.9%, and only 11 cases (15.3%) achieved pathological complete response (pCR). The latter was significantly correlated with the absence of hydronephrosis, low-risk tumors, and associated bilharziasis (p = 0.001, 0.029, and 0.039, respectively). By logistic regression, the high-risk category was the only independent factor associated with a poor likelihood of achieving pCR (OR 4.3; 95% CI 1.1–16.7; p = 0.038). Thirty-day mortality occurred in 5(7%) patients, and 16(22%) experienced morbidity, with intestinal leakage being the most frequent complication. cT4 was the only significant factor associated with post-RC morbidity and mortality compared to cT2 and cT3b (p = 0.01).

Conclusions

Our results are further supporting the radiological and pathological benefits of NAC in MIBC, evidenced by tumor downstaging and pCR. The complication rate after RC is still considerable; hence, more larger studies are necessary to postulate a comprehensive risk assessment tool for patients who would get the maximum benefit from NAC, hoping to accomplish higher complete response rates with ultimately increased adoption of the bladder preservation strategies.
Barriers and enablers for the implementation of trauma-informed care in healthcare settings: a systematic review
Authors: Huo Yan , Couzner Leah , Windsor Tim , Laver Kate , Dissanayaka Nadeeka N. , Cations Monica
Published: May 2023

Background

Healthcare services can be re-traumatising for trauma survivors where they trigger memories of past distressing events and exert limits to a survivor’s sense of autonomy, choice, and control. The benefits of receiving trauma-informed healthcare are well established; however, factors that promote or impede the implementation of trauma-informed care are not yet well characterised and understood.

The aim of this review was to systematically identify and synthesise evidence regarding factors that promote or reduce the implementation of TIC in healthcare settings.

Methods

This systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) 2.0 guidelines. Scopus, MEDLINE, Proquest, PsycINFO and grey literature were searched for original research or evaluations published between January 2000 and April 2021 reporting barriers and/or facilitating factors for the implementation of trauma-informed care in a healthcare setting. Two reviewers independently assessed the quality of each included study using the Mixed Methods Appraisal Tool (MMAT) Checklist.

Results

Twenty-seven studies were included, 22 of which were published in the USA. Implementation occurred in a range of health settings, predominantly mental health services. The barriers and facilitators of implementing trauma-informed care were categorised as follows: intervention characteristics (perceived relevance of trauma-informed care to the health setting and target population), influences external to the organisation (e.g. interagency collaboration or the actions of other agencies) and influences within the organisation in which implementation occurred (e.g. leadership engagement, financial and staffing resources and policy and procedure changes that promote flexibility in protocols). Other factors related to the implementation processes (e.g. flexible and accessible training, service user feedback and the collection and review of initiative outcomes) and finally the characteristics of individuals within the service or system such as a resistance to change.

Conclusions

This review identifies key factors that should be targeted to promote trauma-informed care implementation. Continued research will be helpful for characterising what trauma-informed care looks like when it is delivered well, and providing validated frameworks to promote organisational uptake for the benefit of trauma survivors.

Registration

The protocol for this review was registered on the PROSPERO database (CRD42021242891).
Biphasic positive airway pressure in the management of acute respiratory distress syndrome: a comparative study
Authors: Salem Shymaa Sayed , Hussein Khaled , Badawy M. Sh. , Gad Gad s , Rashad Alaa
Published: May 2023

Background

Biphasic positive airway pressure, also known as BIPAP, is a type of pressure-controlled ventilation that permits unrestricted spontaneous breathing at any time during the ventilatory cycle. Our study’s objective was to compare BIPAP’s effects on ARDS patients with those of the synchronized intermittent mandatory ventilation with pressure control (SIMV-PC) mode.

Results

The present observational, cross-sectional study was conducted on 40 ARDS patients of both sex. These patients were admitted to the intensive care unit, at Qena University Hospital, from April 2019 to November 2021. They were categorized into two groups at random, with group (A) patients receiving BIPAP mode and group (B) patients receiving (SIMV-PC) mode. Changes in arterial blood gases, hemodynamics, and lung mechanics (mean airway pressure, minute volume, tidal volume, compliance, and pressure limit) were compared in both groups after 24 h. There were no differences in the baseline clinical data, demographic, hemodynamic, arterial blood gases, and mortality between the two groups. Follow-up data after 24 h showed that BIPAP was associated with better hemodynamics, oxygenation, and lung mechanics (mean airway pressure, minute volume, and tidal volume). Fewer days of sedation requirements and mechanical ventilation in BIPAP compared to SIMV PC during the duration of the study.

Conclusions

In ARDS patients, BIPAP can improve oxygenation, hemodynamics, lung mechanics, decrease sedation use, and decrease duration of mechanical ventilation.

Trial registration

BIPAP in the Management of Acute Respiratory Distress Syndrome, ID: NCT05483959 Retrospectivelyregistered,https://register.clinicaltrials.gov/prs/app/template/EditRecord.vm?epmode=View&listmode=Edit&uid=U0003OBB&ts=12&sid=S00078AY&cx=9n7oml. Registered on 1 August 2022.
Dexmedetomidine Pretreatment Protects Against Myocardial Ischemia/Reperfusion Injury by Activating STAT3 Signaling
Authors: Chen Zhao-Rong , Hong Yu , Wen Shi-Hong , Zhan Ya-Qing , Huang Wen-Qi
Published: May 2023

BACKGROUND:

Myocardial infarction is a common perioperative complication, and blood flow restoration causes ischemia/reperfusion injury (IRI). Dexmedetomidine (DEX) pretreatment can protect against cardiac IRI, but the mechanism is still insufficiently understood.

METHODS:

In vivo, myocardial ischemia/reperfusion (30 minutes/120 minutes) was induced via ligation and then reperfusion of the left anterior descending coronary artery (LAD) in mice. Intravenous infusion of 10 μg/kg DEX was performed 20 minutes before ligation. Moreover, the α2-adrenoreceptor antagonist Yohimbine and STAT3 inhibitor Stattic were applied 30 minutes ahead of DEX infusion. In vitro, hypoxia/reoxygenation (H/R) with DEX pretreatment for 1 hour was performed in isolated neonatal rat cardiomyocytes. In addition, Stattic was applied before DEX pretreatment.

RESULTS:

In the mouse cardiac ischemia/reperfusion model, DEX pretreatment lowered the serum creatine kinase-MB isoenzyme (CK-MB) levels (2.47 ± 0.165 vs 1.55 ± 0.183; P < .0001), downregulated the inflammatory response (P ≤ .0303), decreased 4-hydroxynonenal (4-HNE) production and cell apoptosis (P = .0074), and promoted the phosphorylation of STAT3 (4.94 ± 0.690 vs 6.68 ± 0.710, P = .0001), which could be blunted by Yohimbine and Stattic. The bioinformatic analysis of differentially expressed mRNAs further confirmed that STAT3 signaling might be involved in the cardioprotection of DEX. Upon H/R treatment in isolated neonatal rat cardiomyocytes, 5 μM DEX pretreatment improved cell viability (P = .0005), inhibited reactive oxygen species (ROS) production and calcium overload (both P ≤ .0040), decreased cell apoptosis (P = .0470), and promoted STAT3 phosphorylation at Tyr705 (0.102 ± 0.0224 vs 0.297 ± 0.0937; P < .0001) and Ser727 (0.586 ± 0.177 vs 0.886 ± 0.0546; P = .0157), which could be abolished by Stattic.

CONCLUSIONS:

DEX pretreatment protects against myocardial IRI, presumably by promoting STAT3 phosphorylation via the α2-adrenoreceptor in vivo and in vitro.
Assessment of Temporomandibular Joint for Occurrence and Severity of Disorders in Adult Cases with Unilateral Cleft Lip and Palate and Non-Cleft Class I Using Helkimo Index
Authors: Kumar Nikhil , Daigavane Pallavi
Published: May 2023

Background: Cleft lip and palate is congenital anomalies. Cleft patients are more akin toward surgical treatment plan neglecting the dental treatment aspects as aesthetic is main concern of patient bond to have malocclusion. Which may contribute to Temporomandibular joint disorder (TMDs) in cleft patients.

Methods: A total 40 patients (20 cleft patients and 20 non cleft class I) were taken in the study. patients’ selection was done based on inclusion and exclusion criteria. Helkimo index was used for both subjective and clinical assessment for TMDs in both groups.

Results: Cleft patients shows significant symptom of TMD between range of mild to severe while non cleft I cases showing mostly mild to moderate symptom of TMDs.

Conclusions: It was found that Unilateral cleft lip and palate (UCLP) cases shows higher occurrence of TMDs in comparison with non-cleft class I group.
El presente desde el pasado o el pasado desde el presente: dos vías complementarias de comprensión del pensamiento arquitectónico contemporáneo.
Authors: Guerra de Hoyos Carmen
Published: May 2023

El texto explora las dificultades de la docencia del pensamiento arquitectónico contemporáneo, intentando encontrar las causas no coyunturales, las debidas a la evolución de la propia arquitectura al adaptarse a las condiciones del contexto socioespacial. Desde esa perspectiva es especialmente importante destacar la insuficiente lectura que la historiografía hace de la transición entre arquitectura moderna y arquitectura contemporánea, como parte de una historia que es necesario narrar todavía. En ese marco se propone la hermenéutica de obras de arquitectura contemporánea como metodología que contribuye por un lado a la comprensión del pensamiento arquitectónico, pero que también, a través de la genealogía, permite enlazar el pasado con el presente y por tanto, superar la brecha que hemos mencionado anteriormente.
Del esteticismo-la posmodernidad: contra la efímera modernidad sXX.
Authors: Saldaña Puerto César
Published: May 2023

La modernidad de la estandarización y el rigor formal compartido, atacada por figuras como Walter Benjamin, Theodor Adorno y Max Horkheimer, Herbert Marcuse o Arnold Hauser, no sólo es la de la tecnocracia y la hegemonía de la razón instrumental en el caso del fascismo y el estalinismo; también fue clave para el establecimiento de otros discursos totalizadores, como en el caso del contexto institucional de Occidente, donde aún alimenta los mitos que conforman nuestra imagen global del sXX. Un siglo que, sin embargo, y como precisamente se propone remarcar este artículo, estuvo dominado desde sus comienzos por tendencias esteticistas y, en su conclusión, por tendencias posmodernas. La fase de superación del relativismo, y los mitos modernos portadores de la ilusión de esta superación —entre los cuales hay que destacar la imagen del movimiento moderno construida desde el discurso institucionalizado de la disciplina arquitectónica moderna—, no fueron más que un efímero tour de force que, aún hoy, retrasan y dificultan nuestra comprensión del presente.
La proyectación por layouts editables. El caso de Bruther.
Authors: Gastambide Federico
Published: May 2023

La planta ha sido probablemente la pieza que dominó históricamente la representación en arquitectura. Orden y control, grillas, ejes y patrones reguladores, entre otros, han garantizado un presunto equilibrio y orden conceptual del proyecto. La planta es una abstracción que ordena y que permite entender un proyecto. En el presente se asiste a una concepción más tensa de la planta, en que se presupone la organización de usos contingentes al mismo tiempo que se conciben. Los dibujos que organizan los usos expresan continuidades, posiciones y relaciones, enlaces y categorías o superposiciones, son diagramas que manifiestan la mecánica de los potenciales (y cambiantes) usos. Esta lectura permite ver a la arquitectura como un elemento adaptativo en el que nunca se comienza desde cero, el origen es un momento reactivo, es la reinterpretación de algún otro diagrama que se retoma, que se formatea hacia otros usos. Al respecto pareciera repetirse en varias oficinas de arquitectura un modo de proyectación híbrido analógico-digital denominado layouts editables. Éste afirma el valor de un cierto oficio más operativo y acelerado, distante de las concepciones paramétricas y procesuales más recientes. Este modo presupone que el proyecto no tiene pretensiones totalizadoras y no es definitivo o cerrado. Sus creadores asumen que el proyecto se transformará, se reconfigurará y quizá solo podrá realizarse parcialmente, tal concepción se acerca a una cierta sumisión creativa. En tal caso emerge la dificultad de la crítica de explicar un proyecto que en poco tiempo será otro.
REFLEXÕE SOBRE O SUL-SUDESTE-CENTRISMO EM RELAÇÃO AOS ESTUDOS DO ENSINO DE HISTÓRIA E HISTÓRIA DIGITAL: UMA CONVERSA COM MARCELLA ALBAINE, HSTÉFFANY MUNIZ E CLEICIMAR SOUZA.
Authors: Peçanha Natália Batista , Alexandre Maria Lúcia Bezerra da Silva , Alexandre Maria Lúcia Bezerra da Silva
Published: May 2023

Entrevista realizada com as professoras Marcella Albaine, Hztéffany Muniz e Cleicimar Souza, acerca da importância de se refletir sobre o sul-sudeste-centrismo da produção do ensino de História e História Digital, enfatizando a produção intelectual do norte do páis, em especial, de Roraima.
O QUE SIGNIFICA SER UM PROFESSOR YOUTUBER DE HISTÓRIA? APONTAMENTOS SOBRE ATIVIDADE DOCENTE, PRÁTICAS E VISUALIDADES PARA O ENSINO DE HISTÓRIA NO YOUTUBE
Authors: Rocha Pedro Botelho
Published: May 2023

Através da análise de entrevistas de três professores que produzem vídeos de História para o site do YouTube, este presente trabalho tem como objetivo investigar a categorização de um determinado perfil docente, na qual a literatura do Ensino de História e Mídias Digitais têm chamado de Professor Youtuber, destacando suas características e elementos didáticos, pedagógicos, metodológicos e historiográficos que perpassam sua atividade. Também pretendemos enfocar aspectos do próprio entendimento e auto-avaliação que esses professores possuem de suas produções audiovisuais dentro de uma mídia social digital de relevância, como é o site do YouTube, e suas possíveis conexões com o universo de influenciadores digitais, personalidades da cultura digital.
ENSINO DE HISTÓRIA NA EDUCAÇÃO BÁSICA E A EXPANSÃO DO CAPITAL IMPERIALISMO NO CIBERESPAÇO
Authors: Brinco Naicon De Souza
Published: May 2023

A partir do campo do ensino de História e do conceito de território o presente artigo discute a expansão dos oligopólios de tecnologia no fazer cotidiano dos professores de História da educação básica com o advento da pandemia de COVID-19 e das medidas de distanciamento social nos anos de 2020 a 2022. As reflexões partem da experiência em salas de aula nos doze anos como professor da educação pública e de dados globais acerca do uso do digital pelos estudantes. Apresentamos como resultado a potência do ensino de História na desnaturalização não só dos aparelhos da tecnologia, mas das práticas culturais de uso dos espaços digitais ocupados pelos alunos.
O ENSINO DE HISTÓRIA EM ENCRUZILHADA: UM ENCONTRO ENTRE A SALA DE AULA INVERTIDA E AS TECNOLOGIAS DIGITAIS
Authors: Casali de Oliveira Medeiros Leandro , Spinosa Vanessa
Published: May 2023

Este artigo busca investigar as contribuições de uma metodologia ativa de ensino híbrido, a sala de aula invertida, para o ensino de história escolar, mediado por Tecnologias Digitais da Informação e Comunicação (TDICs). Para isso, busca-se estabelecer um diálogo entre as reflexões da história pública e da didática da história de Rüsen enquanto chaves de leitura para compreender e analisar criticamente essa metodologia, com base numa experiência com alunos do 2o ano do Ensino Médio de uma instituição privada de ensino na cidade do Rio de Janeiro, no ano de 2021.
O LETRAMENTO DIGITAL EM TEMPOS DE PLATAFORMIZAÇÃO DA EDUCAÇÃO E AS POSSIBILIDADES PARA O ENSINO DE HISTÓRIA.
Authors: Rodrigues de Oliveira Max Fabiano
Published: May 2023

ResumoEste artigo reflete sobre a importância do letramento digital de professoras e professores que atuam no ensino básico. Além de observar a importância de um letramento midiático que dê conta não apenas de questões operacionais, mas também se debruce para o estudo do funcionamento da rede em relação a privacidade de dados de crianças e adolescentes em um cenário de plataformização da educação e comportamento algorítmico que customiza aquilo que deve ou não aparecer em feeds infinitos. Há uma grande necessidade de formação continuada que busque o aperfeiçoamento desses docentes no que se refere ao uso das novas tecnologias digitais de informação e comunicação, as chamadas (NTICs). Letrados digitalmente, professores e estudantes, tornam-se peças fundamentais para combater a disseminação do discurso de ódio, fake news e a desinformação. Palavras-chave: Letramento digital; fake news; discurso de ódio; ensino de história
Infographic: Total hip arthroplasty in early osteoarthritis
Authors: Sharrock Martin , Board Tim
Published: May 2023

Cite this article: Bone Joint Res 2023;12(5):309–310.
Short Communication: Diversity of dragonflies (Ordo: Odonata) on the natural reserve areas of Mt. Sigogor and Mt. Picis, Ponorogo District, Indonesia
Authors: PRANOTO MALINDA DUTA PERTIWI , MARDIONO DHENY , WIDIYANI TETRI , PERTIWI RIZQI ADANTI PUTRI , AZ ZHARA FATIMAH , IZZATI NAILA
Published: May 2023

Abstract. Pranoto MDP, Mardiono D, Widiyani T, Pertiwi RAP, Az Zhara F, Izzati N. 2019. Short Communication: Diversity of dragonflies (Ordo: Odonata) on the natural reserve areas of Mts. Sigogor and Picis, Ponorogo District, Indonesia. Bonorowo Wetlands 9: 27-31. The Mount Sigogor and Picis Natural Reserves are the conservation areas in Ponorogo, East Java. Mount Sigogor and Picis nature reserves have an ecosystem of tropical rain forests that are naturally protected, providing the reserve with a high potential for biodiversity. The preserved ecosystem conditions are a good habitat for the dragonfly since some dragonfly species require a clean habitat and are sensitive to pollutants. The study was conducted from January to February 2019. The research site was carried out at 7 points, covering 5 points in the reserve and 2 points around Mount Sigogor Nature Reserve. Data retrieval was done using the explorative method. Qualitative and quantitative descriptions were used to analyze the results of dragonflies’ biodiversity. The results have found 18 species of dragonflies with details of 6 species as damselflies (Zygoptera) and 12 species of common dragonflies (Anisoptera). There are 5 species of endemic dragonflies from Java Island, i.e., Drepanosticta sundana, Euphaea variegata, Heliogomphus drescheri, Heliocypha fenestrat, and Vestalis luctuosa. From the Shannon-Wiener discounting index, the value of index diversity on the entire research site is 1.466. The highest diversity value lies in the river's location leading to Toyo Marto's waterfall with a 2.02 diversity-index value. Obtained results that the Euphaea variegata has the most abundant with a 40.23% value.
Isolation and identification of phosphate solubilizing bacteria from the rhizosphere of rice in organic and non-organic rice fields in Sukoharjo District, Indonesia
Authors: PURWANTI DWI , SETYANINGSIH RATNA , SUSILOWATI ARI
Published: May 2023

Abstract. Purwanti D, Setyaningsih R, Susilowati A. 2019. Isolation and identification of phosphate solubilizing bacteria from the rhizosphere of rice in organic and non-organic rice fields in Sukoharjo District, Indonesia. Bonorowo Wetlands 9: 108-115. One of the essential nutrients in the soil is phosphate. Phosphate fertilization is often inefficient because phosphate is bound and difficult for plants to absorb. The use of phosphate solubilizing bacteria (PSB) can be used as an alternative to the availability of P in the soil. This study aimed to obtain PSB isolates with high phosphate solubilization ability and identify these PSB isolates molecularly. Soil samples were taken from organic and non-organic rice (Oryza sativa L.) fields in Sukoharjo, Central Java, Indonesia, namely Grogol Village, Menuran Village, and Pondok Village. The research activity was carried out in several stages: isolation of PSB on Pikovskaya medium, characterization and testing of PSB in dissolving P, and Gram staining. PSB colonies with a phosphate solubilization index and showed different colony colors were taken, and then DNA was isolated. DNA isolation was carried out by extraction method using a DNA extraction kit. The gene encoding 16S rRNA amplified the obtained DNA samples. Sequencing was carried out using a genetic analyzer. Data analysis by comparing sequence data on the BLASTN program. Based on the isolation of rhizosphere samples from organic paddy fields, 14 isolates of PSB were obtained, and ten isolates of non-organic paddy were PSB. P13, P1, Q7, and Q8 isolates had the highest P solubilization ability with dissolution index (IP), namely 3.35, 2.13, 1.82, and 1.47. The 16S rRNA gene sequence analysis showed that isolates P1 and P13 had 99% similarity to Acinetobacter sp. ADP1 strain. The isolate Q7 had a 96% similarity with the genus Clavibacter strain NCPPB 382, ??while Q8 had a 99% similarity with Pseudomonas aeruginosa strain PAO1.
Isolation and identification of lipolytic bacteria from the digestive tract of eel (Anguilla bicolor bicolor)
Authors: HAPSARI RHISMA TRY , SUSILOWATI ARI , PANGASTUTI ARTINI
Published: May 2023

Abstract. Hapsari RT, Susilowati A, Pangastuti A. 2020. Isolation and identification of lipolytic bacteria from the digestive tract of eel (Anguilla bicolor bicolor). Bonorowo Wetlands 10: 44-50. Anguilla bicolor bicolor is commonly found in the waters of western Indonesia. Currently, the demand for eel is increasing, causing an increase in the economic value of eel and being used as an export commodity. This study aims to isolate and identify lipolytic bacteria found in the digestive tract of eel (A. bicolor bicolor). Lipolytic bacteria isolated from the digestive tract of eel (A. bicolor bicolor). Bacterial isolation uses minimal media enriched with olive oil. When lipolytic bacteria were screened using Rhodamine B agar media, the presence of an orange glow in the colony upon exposure to 350 nm UV light indicated lipolytic activity. Identification of lipolytic bacteria was based on observing the morphological characters of bacterial colonies (edges, elevation, colonies, and colors) and the gene sequences encoding 16S rRNA. DNA sequence characteristics of lipolytic bacteria were analyzed by BLAST Nucleotide on the NCBI website (www.blast.ncbi.mlm.nih.gov/blast.cgi). Based on the screening results, 7 isolates of lipolytic bacteria were obtained from 30 successfully isolated isolates. The DNA sequence characteristics of the bacterial isolates were analyzed by BLASTN and found 5 species, namely Pseudomonas azotoformans, Providencia vermicola, Providencia sp., Aeromonas veronii, and Aeromonas hydrophila.
POČÍTAČOVÁ SIMULACE ZRANĚNÍ CHLAPCE NA ZADNÍM SEDADLE OSOBNÍHO VOZIDLA
Authors: Pavlata Petr
Published: May 2023

Příspěvek se zabývá využitím moderních simulačních technik s podporou sw VPS (Virtual Performance System) a škálovatelnýho biomechanickým modelem lidského těla Virthuman při řešení silniční nehody s těžkým zraněním chlapce, sedícího na dětské sedačce na zadním sedadle osobního vozidla. Závěry analýzy naznačují, že chlapec v rozporu s předchozím tvrzením nebyl připoután. S podporou a využitím výše uvedeného sw a modelu, bylo možné zprěsnit výpočtové vymezení střetové rychlosti silničního vozidla.
A New Archaeological Response to an Old Question: When and how Did Ur Recover in the Old Babylonian Period?
Authors: Otto Adelheid
Published: May 2023

The destruction of the city of Ur at the end of the Ur III period was so radical that it remained in the collective memory for centuries. But when and how did Ur recover in the Old Babylonian period and became important again? New research on the South Mound of Ur provides fresh answers to this old question. The area in the southern part of the South Mound shows a hiatus in occupation between the Ur III period and the 19th century and suggests that a new living quarter was designed here after a period of abandonment. Combined with historical sources attesting to a Larsa dynasty restoration programme and the creation of new residential areas, we argue that Ur did not fully recover from the aftermath of destruction until the mid-19th century.
Microwave target location method based on the diamond NV color center
Authors: Liu Yusong , Wang Qi , Qin Yue , Guo Hao , Li Jie , Li Zhonghao , Wen Huanfei , Ma Zongmin , Tang Jun , Liu Jun
Published: May 2023

We propose a method for microwave target source localization based on the diamond
nitrogen vacancy color center. We use coherent population oscillation
effect and modulation and demodulation techniques to achieve the
detection of microwave intensity of microwave target sources, with a
minimum detection intensity of 0.59 µW. Positioning of
the microwave source was achieved within 50×100cm2 distance from the system 1 m
away using the cubic spline interpolation algorithm and minimum mean
squared error. The maximum positioning error was 3.5 cm. This
method provides a new, to the best of our knowledge, idea for the
passive localization of microwave targets.
Modeling SARS-CoV-2 antibody seroprevalence and its determinants in Ghana: A nationally representative cross-sectional survey
Authors: Owusu Donkor Irene , Mensah Sedzro Kojo , Dwomoh Duah , Akorli Jewelna , Abuaku Benjamin , Ashong Yvonne , Opoku Millicent , Andoh Nana Efua , Sumboh Jeffrey Gabriel , Ohene Sally-Ann , Owusu-Asare Ama Akyampomaa , Quartey Joseph , Dumashie Edward , Lomotey Elvis Suatey , Odumang Daniel Adjei , Gyamfi Grace Opoku , Dorcoo Christopher , Afatodzie Millicent Selassie , Osabutey Dickson , Ismail Rahmat bint Yussif , Quaye Isaac , Bosomprah Samuel , Munster Vincent , Koram Kwadwo Ansah
Published: May 2023

Estimates of SARS-CoV-2 transmission rates have significant public health policy implications since they shed light on the severity of illness in various groups and aid in strategic deployment of diagnostics, treatment and vaccination. Population-based investigations have not been conducted in Ghana to identify the seroprevalence of SARS-CoV-2. We conducted an age stratified nationally representative household study to determine the seroprevalence of SARS-CoV-2 and identify risk factors between February and December 2021. Study participants, 5 years and older regardless of prior or current infection COVID-19 infection from across Ghana were included in the study. Data on sociodemographic characteristics, contact with an individual with COVID-19-related symptoms, history of COVID-19-related illness, and adherence to infection prevention measures were collected. Serum obtained was tested for total antibodies with the WANTAI ELISA kit. The presence of antibodies against SAR-COV-2 was detected in 3,476 of 5,348 participants, indicating a seroprevalence of 67.10% (95% CI: 63.71–66.26). Males had lower seroprevalence (65.8% [95% CI: 63.5–68.04]) than females (68.4% [95% CI: 66.10–69.92]). Seroprevalence was lowest in >20 years (64.8% [95% CI: 62.36–67.19]) and highest among young adults; 20–39 years (71.1% [95% CI 68.83,73.39]). Seropositivity was associated with education, employment status and geographic location. Vaccination status in the study population was 10%. Exposure is more likely in urban than rural areas thus infection prevention protocols must be encouraged and maintained. Also, promoting vaccination in target groups and in rural areas is necessary to curb transmission of the virus.
Comparative analysis of retinal photoplethysmographic spatial maps and thickness of retinal nerve fiber layer
Authors: Odstrcilik Jan , Kolar Radim , Horn Folkert , Tornow Ralf-Peter
Published: May 2023

The paper presents a comparative study of the pulsatile attenuation amplitude (PAA) within the optic nerve head (ONH) at four different areas calculated from retinal video sequences and its relevance to the retinal nerve fiber layer thickness (RNFL) changes in normal subjects and patients with different stages of glaucoma. The proposed methodology utilizes processing of retinal video sequences acquired by a novel video ophthalmoscope. The PAA parameter measures the amplitude of heartbeat-modulated light attenuation in retinal tissue. Correlation analysis between PAA and RNFL is performed in vessel-free locations of the peripapillary region with the proposed evaluating patterns: 360° circular area, temporal semi-circle, nasal semi-circle. For comparison, the full ONH area is also included. Various positions and sizes of evaluating patterns in peripapillary region were tested which resulted in different outputs of correlation analysis. The results show significant correlation between PAA and RNFL thickness calculated in proposed areas. The highest correlation coefficient Rtemp = 0.557 (p<0.001) reflects the highest PAA-RNFL correspondence in the temporal semi-circular area, compared to the lowest value in the nasal semi-circular area (Rnasal = 0.332, p<0.001). Furthermore, the results indicate the most relevant approach to calculate PAA from the acquired video sequences is using a thin annulus near the ONH center. Finally, the paper shows the proposed photoplethysmographic principle based on innovative video ophthalmoscope can be used to analyze changes in retinal perfusion in peripapillary area and can be potentially used to assess progression of the RNFL deterioration.