bluuebunny/crossref_metadata_2025 huggingface dataset:
State Ownership and Risk-Taking Behaviour: Evidence from Malaysia’s Banking Industry
Authors: Lee Ai-Xin , Hooy Chee-Wooi
Published: January 2021

This study investigates state ownership on risk-taking behaviour in Malaysia’s banking industry. Using the panel of Malaysian commercial banks, this paper examines whether banks’ risk-taking is affected by Malaysian government ownership through the five largest investment arms of Malaysia (GLICs). The findings show that state-owned banks exhibit higher risk-taking behaviour compared to the private-owned banks in terms of loans. There is evidence that a higher degree of state ownership has a more significant impact on banks’ risk-taking behaviour. We also investigate the relationship with corporate governance mechanisms. The findings suggest that the composition of board of directors somehow plays a significant role in the governance of banks.
MOLECULAR SCREENING OF SOME ROMANIAN APPLE CULTIVARS FOR SCAB RESISTANCE GENES
Authors: Militaru Mădălina , Sturzeanu Monica , Iancu Adina
Published: January 2021

Apple scab, incided by the fungus Venturia inaequalis (Cke.) Wint., is a devastating disease of apple reported from almost all apple producing Romanian areas, which causes up to 70% losses of production. Molecular markers were used for detection of scab resistance genes in 22 old and introduced apple cultivars ('Romus 3', 'Romus 5', 'Rebra', 'Rustic', 'Nicol', 'Colmar', 'Colonade', registered by Research Institute for Fruit Growing Pitesti; 'Generos', 'Iris', 'Irisem', 'Luca', 'Ciprian', 'Cezar', 'Remar', 'Valery', 'Real', registered by Research Station for Fruit Growing Voinesti, Dambovita; 'Aura', 'Starkprim', 'Ionaprim', 'Bistritean', registered by Research Station for Fruit Growing Bistrita and old cultivars: 'Domnesc', 'Cretesc'). The presence of scab resistance genes were detected using the molecular markers: AL-07 (SCAR), AM19 (SCAR), VfC for Rvi6 (Vf) gene, AD13 (SCAR) for Rvi4 (Vr1) gene, OPL19 (SCAR) for Rvi2 (Vh2) and Rvi8 (Vh8) genes and OPB12 (STS) for Rvi5 (Vm) gene. The Rvi6 gene was detected in 17 cultivars from different breeding center. The marker AD13 presents in genome of 8 cultivars, such as 'Romus 3', 'Romus 5', 'Generos', 'Iris', 'Irisem', 'Cezar', 'Remar', 'Aura'. The Rvi5 gene was revealed in 3 cultivars ('Nicol', 'Generos', 'Irisem'), only.
GENETIC DIVERSITY IN SELECTED PROGENY OF PLUM CULTIVAR ‘TULEU GRAS’
Authors: Butac Mădălina , Chivu Mihai , Mareși Eugenia
Published: January 2021

Becoming from several species and influenced by the very varied climate and soil conditions that characterize the Romanian territory, the plum has developed in this area numerous biological forms with a wide range of characters and characteristics. Taking into account these realities, fruit growers have selected valuable forms with an amazing diversity in terms of taste, size, productivity, ripening season, resistance to biotic and abiotic factors. Of these, the 'Tuleu gras' variety, due to its high plasticity and fruit quality, was very widespread in orchards in Romania and was used as parent in the autochthonous plum breeding since the second half of the last century, representing a numerous source of genes. Thus, by using this variety in breeding works, so far 23 varieties with a great genetic diversity have been registered. The aim of this paper was to study the phenotypic variability of some of these descendants. Thus, many varieties created in this way from 'Tuleu gras' (‘Piteștean’, ‘Centenar’, ‘Tita’, ‘Roman’) certainly recorded a genetic gain in almost all objectives, being in turn used as parents in the current works plum improvement.
Efficient Synthesis of Pharmaceutically Relevant Prochiral Heterocyclic Aminoketones
Authors: Lakó Ágnes , Poppe László , Mendonça Ricardo
Published: January 2021

In this work, we report a practical method for alkylation of saturated heterocycles with chloroacetone yielding prochiral heterocyclic ketones, including previously not described molecules. The desired building blocks were obtained with high yields in hydrochloric salt forms, without the need for chromatographic purification.
Power-HIL Application Analysis of a 3-level Inverter for PMSM Machine
Authors: Kiss Dávid , Varjasi István
Published: January 2021

Power-HIL simulation is one of the emerging areas in power electronics development nowadays. It offers a convenient test environment for the whole power electronics hardware but eliminates the necessity of motor test benches and rotating machines. Selecting a suitable power amplifier for the simulator is however a challenging task. Switching power supplies can be an interesting option as Power Amplifier, but they have to offer superior power capability and dynamic performance over the DUT (Device Under Test), while maintaining high enough switching frequency to meet the dynamic requirements as well. Using commercially available inverters as Power Amplifiers would be an attractive option, if they can achieve the desired emulation accuracy. This paper investigates the possibility of using a common 3-level inverter with an L-C-L coupling network as a Power Amplifier for a P-HIL simulator, to emulate a PMSM (Permanent Magnet Synchronous Machine) machine.
The Social Art of Language Acquisition: A Theatre Approach in Language Learning for Migrants and its Digitization in the Corona Lockdown
Authors: Best Christine , Guhlemann Kerstin , Argemí Guitart Ona
Published: January 2021

Today's challenges and developments ? migration flows, globalization, diversification of educational pathways – clearly indicate that language teaching can no longer only take place within the framework of school and vocational training. Outside the context of schools, approaches which combine language learning with other goals of development or that are linked to concrete objectives appear most promising. A particularly challenging field is teaching languages to adult refugees, since language courses can seldom address the learning culture, the different learning speeds and key competencies adequately. In the wake of the current Corona crisis, the problem is becoming more pressing. By digitalization of language courses, there is the danger that people with better prerequisites benefit more from the offered courses than those more in need, and the gap between people with higher or lower learning success will widen further. The article uses the practical example JobAct Sprachkultur, which is a German programme for job placement, based on a combination of theatre methods, language learning and social work, to investigate the extent to which theatre methods can contribute to reducing educational inequality in the language acquisition of adult refugees/migrants. Based on a document analysis and qualitative spotlight surveys with the trainers of the program, the potentials and limitations of the theatre approach and its digital implementation can be shown.
Theater der Unterdrückten – analog und digital!
Authors: Hoffelner Alexander , Scheidl Walter
Published: January 2021

Medienbildung, Politische Bildung und Theaterpädagogik teilen einige wesentliche Gemeinsamkeiten, sowohl in struktureller Hinsicht als auch in Bezug auf ihre Ziele. Der Beitrag erörtert diese Schnittstellen anhand grundlegender pädagogisch-didaktischer Literatur und stellt dar, dass es sowohl in der Politischen Bildung, der Medien- wie auch in der Theaterpädagogik um eine Ermächtigung Lernender geht, sich gegen ungleiche Machtverhältnisse (in der analogen und digitalen Welt) zur Wehr zu setzen und selbst gestalterisch tätig zu werden. Neben der theoretischen Erörterung dieser Verbindungen werden praktische Konzepte für den Unterricht präsentiert.
COVID-19 Australia: Epidemiology Report 32: Four-week reporting period ending 3 January 2021
Authors:   COVID-19 National Incident Room Surveillance Team
Published: January 2021

This is the thirty-second epidemiological report for coronavirus disease 2019 (COVID-19), reported in Australia as at 23:59 Australian Eastern Daylight Time [AEDT] 3 January 2021. It includes data on COVID-19 cases diagnosed in Australia and the international situation.
A presença da Medicina Legal como disciplina nos cursos de Direito: análise de sua importância para o Direito Processual Penal e a expertise do sistema de justiça brasileiro, aludindo ao caso Isabella Nardoni
Authors: Oliveira Mariana Ferreira de , Oliveira Daniela Ferreira de , Teles José Cledson Paciência , Limeira Ronaldo Barbosa , Silva Lucas Nunes de Brito
Published: January 2021

Objetivo: O presente artigo tem por escopo analisar a presença da disciplina de Medicina Legal (ML) nas Instituições de Ensino Superior (IES) da região nordeste brasileira, bem como melhor elucidar a influência positiva que a ML possui na resolução de casos concretos do processo penal. Metodologia: Por meio da base de dados eletrônica do Ministério da Educação (e-MEC), coletou-se todas as IES nordestinas com graduação em Direito, seguido pela consulta da grade curricular no site de cada instituição. Foram avaliados presença da disciplina ML na matriz curricular, sua carga horária, obrigatoriedade de ensino e período em que é lecionada. Além disso, analisou-se o caso do “casal Nardoni”, discutindo a imprescindibilidade da ML para o alcance da verdade processual real. Resultados: Incluíram-se 264 faculdades, onde apenas 91 possuem disciplina exclusiva para o ensino da mesma. Dentre as faculdades em que é lecionada, a sua obrigatoriedade está presente em apenas 45,7% das graduações. A carga horária média foi de 49,7 horas/aula, representando somente 1,2% do total de horas da estrutura curricular. Conclusão: É possível constatar que o ensino da Medicina Legal não está em preponderância nas IES do nordeste, cabendo ao bacharel em direito buscar formação complementar sobre o tema.
Revisão: Implantação das boas práticas de fabricação na indústria Brasileira de alimentos
Authors: Oliveira Patricia Ocampos  de , Silveira Roberta da , Alves Eloize Silva , Saqueti Bruno Henrique Figueiredo , Castro Matheus Campos de , Souza Patricia Magalhães de , Ponhozi Isadora Boaventura , Costa Joice Camila Martins da , Schueler Janaina , Santos Oscar Oliveira , Visentainer Jesui Vergilio , Duailibi Sandra Rojas
Published: January 2021

Segurança dos alimentos abrange conhecimentos e práticas referentes à saúde coletiva, com intuito de prevenir riscos associados à alimentação. Adjacente está o controle de qualidade, que associa fundamentos básicos para que o processo de produção de alimentos seja realizado evitando o surgimento de doenças transmitidas por alimentos (DTAs). DTAs são originadas pela ingestão de alimentos ou água contaminados, onde diversos fatores colaboram para emergência de doenças. Com isto, a Vigilância Sanitária apresenta como um dos papeis de fiscalização para liberação de licença sanitária. Sendo assim, o presente trabalho trata-se de uma revisão na literatura baseada nos programas de controle de qualidade em função da segurança dos alimentos com ênfase nas doenças transmitidas por alimentos. Para garantir a higiene dos produtos e a segurança dos alimentos, existem diversos programas, tais como: Boas Práticas de Fabricação (BPF) têm como objetivo evitar a contaminação dos produtos, desde a recepção das matérias-primas até o consumo. Programa de Autocontrole (PAC) principal ferramenta da agroindústria no controle dos processos de fabricação. Procedimento operacional padronizado (POP) consiste em descrever todas as operações para realizar determinado procedimento. Sistema de análise de perigos e pontos críticos de controle (APPCC) baseia-se na aplicação de princípios técnicos e científicos de prevenção, com finalidade de garantir inocuidade dos processos de produção, manipulação, transporte, distribuição e consumo dos alimentos. E método 5S consiste em organizar o local de trabalho por meio de manutenção, limpeza, padronização e disciplina na realização do trabalho, com o mínimo de supervisão possível. Demonstra-se que todos programas caminham juntos.
Desejo de performance na arena discursiva da desprofissionalização docente
Authors: Brittes Letícia Ramalho , Costa Joacir Marques da , Lima Cléber Lixinski de
Published: January 2021

O princípio da gestão democrática no sistema educacional brasileiro avança, discursivamente, mediante direcionamentos da Constituição Federal de 1988 e da Lei de Diretrizes e Bases n° 9.394/96, o que, de certo modo, movimenta re/configurações do trabalho docente. E é a partir desse contexto que repousa o presente estudo, tendo como interesse problematizar como o enfoque democrático/neoliberal e suas dissoluções de sentidos têm operado paralelamente à cultura da performatividade, delineando novos papéis e novos perfis para o trabalho docente. Para tanto, realizou-se uma pesquisa de cunho bibliográfico para subsidiar a discussão em torno das noções de trabalho, gestão e da arena discursiva global e local das políticas educacionais. Concluiu-se que, quando uma cultura performativa opera sobre o trabalho docente, desencadeiam-se sentidos de desprofissionalização, atrelados, sobretudo, a um processo de autonomia imaginada, o que poderá gerar maior controle e adesão por parte do professorado às políticas de responsabilização docente. Nesse sentido, o professor é peça fundamental para o funcionamento discursivo das políticas, haja vista o possível engajamento desse sujeito relacionado, tanto, ao desejo de performance, quanto, para delinear os rumos da recontextualização dessas políticas, as quais poderão conduzir ao sucesso e/ou fracasso escolares.
Rehabilitation and reintegration policies of children victims of criminal action in the Children's Criminal Jurisdiction System
Authors: Purwati Ani , Purwaningtyas Fifin Dwi , Agung Jumali Sapta
Published: January 2021

This research examines the harmonization of statutory regulations and the application of law on child victim protection as an achievement of optimal independence for child victims during formal restorative justice and post-recovery. The research objectives and specific targets were (1) formulating the concept of rehabilitation and reintegration of child victims in the juvenile justice system; (2) establishment of SOP (Standard Operational Implementation) for law enforcement officers on the handling of child victims; (3) integrated networking institutions / legal aid institutions and psychological assistance for child victims; and (4) the formulation of juvenile justice teaching, restorative justice, legal and psychological approaches. The method used in this research is through a mix methodological approach between law and psychology with a conceptual approach and a statue approach to analyze the weaknesses of current legislation, such as centralized legal protection for child victims of the fulfillment of restorative judicial evidence (informal), but not yet. Integrated psychosocial and vocational rehabilitation programs for every victims. Apart from that, the child criminal law policy in Indonesia which implements restorative justice is still in the perspective of children in conflict with the law. The concept method of victim rehabilitation is implemented by rehabilitation programs in accordance with the principles of the Convention on the Rights of the Child (KHA).
Evaluation of the omission of nutrients in the mineral development and composition of young cupuaçuzeiro plants (Theobroma grandiflorum (Willd. ex Spreng.) Schum.) progeny 61
Authors: Almeida Gabriela Mourão , Viégas Ismael de Jesus Matos , Alves Rafael Moysés , Silva Henrique Barata da , Silva Alasse Oliveira da , Silva Diocléa Almeida Seabra
Published: January 2021

Include the abstract in English. (TNR font 10 – simple space) The objectives of the study were to evaluate the fertility of the Yellow Latosol medium texture in plants of cupuassu (Theobroma grandiflorum) progeny 61 and to characterize the visual symptoms of macronutrient and micronutrient deficiencies. The plants were grown in nutrient solution containing all macronutrients and micronutrients and in solutions with omission of N, P, K, Ca, Mg, S, B, and Zn, using the missing element technique. The experimental design was completely randomized with eleven treatments and five replications. Growth was evaluated through production and dry matter at 60 days after planting. The progeny showed market potential, as it is more productive than the others, but it presents nutritional requirements similar to those of the cultivars already studied. The nutrient required in greater quantities by the culture was nitrogen, but the omission, but limiting, was that of potassium for biometric variables. The best treatment was complete followed by omission of zinc, but the difference between them was significant. The levels of nutrients in the leaves were similar to those found by other authors in other cultivars. The decreasing order of treatments was C> Zn> T> N> P> Ca> Mg> S> B> K.
Estudo retrospectivo de surtos de doenças veiculadas por alimentos, na região nordeste e Estado do Maranhão, no período de 2007 a 2019
Authors: Souza Jaqueline Freitas , Souza Antonio Carlos Freitas , Costa Francisca Neide
Published: January 2021

A incidência de doenças veiculadas por alimentos cresce a cada ano no Brasil, porém estima-se que um número elevado de casos não é notificado. O objetivo deste trabalho será fazer um estudo retrospectivo   da ocorrência de surtos de doenças veiculadas por alimentos, no estado do Maranhão, período de 2007 a 2019, onde foi realizado um estudo descritivo do tipo transversal, utilizando-se como base de dados o Sistema de Informação de Agravos de Notificação – SINAN. No período estudado foram identificados 48 surtos de doenças de origem alimentar no estado do Maranhão, com 2.535 doentes. Os agentes etiológicos identificados foram a Salmonella sp. (6,25%), Escherichia coli (4,17%), rotavírus (4,17%), Staphylococcus aureus e coliformes, ambos com 2,08%, porém, em 81,25% dos surtos, o agente etiológico não foi identificado. A residência foi o local de ocorrência com maior número de surtos (37,5%), seguindo de casos dispersos no bairro (12,5%) e restaurante/padaria (10,42%), ainda é importante citar a ocorrência de 3 surtos (6,25%) em hospitais/Unidade de saúde. Quanto à origem, em 47,75% dos surtos, o alimento envolvido no surto não foi identificado e a água e alimentos mistos foram os mais comuns, com frequência de 14,58% e 12,5%, respectivamente. Quanto aos critérios de confirmação, em 52,08% dos surtos foi utilizado o critério clínico-epidemiológico, 14,58% realizaram análises bromatológicas para confirmação e 10,42% apenas critérios clínicos, inconclusivos e ignorados representaram 22,92%.  As diferenças na quantidade de surtos notificados no período do estudo podem decorrer de uma possível subnotificação ocasionando lacunas nos resultados encontrados.
Divulgação de informações sobre higiene e mudança de hábitos durante a pandemia da Covid-19
Authors: Rodrigues Noádia Priscila Araújo , Silva Daniella Rocha da , Garcia Júnior Élcio Antônio , Silva Júnior Edmilson Fernandes da , Gomes Renally da Silva , Fernandes Karina Felix Dias , Oliveira Kataryne Árabe Rimá de
Published: January 2021

Covid-19 é uma doença respiratória aguda, causada pelo vírus SARS-CoV-2, que foi categorizada como pandemia em 2020. Sua contaminação acontece através de gotículas do vírus ativo, que pode ser transmitido entre indivíduos ou através de objetos contaminados. Mediante esses aspectos o presente trabalho teve como objetivo verificar se a busca por informações sobre medidas de higiene promoveu mudanças de hábitos nas práticas de higiene durante a pandemia da Covid-19. Para isso foi realizado um estudo transversal, através de questionário on-line (na plataforma Google formulários), que continha perguntas relacionadas ao período de emergência sanitária. A análise dos dados foi realizada através de estatística descritiva e inferencial por meio do teste de Qui-Quadrado de Pearson. Dentre os respondentes, a maioria declarou buscar informações sobre higiene para mitigar a contaminação do coronavírus durante o período da pandemia, com destaque para informações específicas sobre higiene de alimentos. As estratégias mais utilizadas para obter informação sobre práticas de higiene foram: internet, telejornais e e-book sobre boas práticas de prevenção ao Coronavírus. Dentre essas a internet influenciou positivamente nos hábitos de higiene de alimentos e uso correto de máscara. Ainda foi observado que os participantes mudaram os hábitos de aquisição de alimentos no que diz respeito a observar o prazo de validade e higienizar as embalagens no domicilio. É possível concluir que o período de pandemia gerou mudanças importantes nos hábitos de higiene da população estudada, e que estratégias de educação sanitária podem gerar transformações positivas nas práticas de produção de alimentos seguros.
Uma aplicação ao setor de transporte agrícola da análise de confiabilidade
Authors: Santos Isaac Pereira , Almeida Pablo Lourenço Ribeiro de , Silva Deise Pereira da , Fidelis Cleanderson Romualdo , Medeiros Elias Silva de , Oliveira Tiago Almeida de
Published: January 2021

A análise de confiabilidade é a conversão de técnicas de análise de sobrevivência aplicadas no departamento de produção. Para realizar a análise de confiabilidade, o conjunto de dados em estudo deve atender às condições necessárias para que seja possível fazer estimativas razoáveis ​​das funções de risco e confiabilidade. O objetivo deste trabalho é utilizar a análise de confiabilidade, utilizando as técnicas não paramétricas, semi-paramétricas e paramétricas, para explorar e modelar o tempo de do pneu 11.00R22. Foi realizado um estudo com os 11.00R22, e o tempo em quilômetros rodados foi calculado por meio de um computador de bordo acoplado em caminhões. Os conjuntos de dados têm um total de 552 pneus, os quais apresentaram diferença entre os três tipos de tempo de vida. Na execução deste trabalho, aplicou-se o estimador limite produto de Kaplan-Meier para os três grupos de vida, e o teste log-rank para verificar a existência de diferença significativa entre as curvas de sobrevivência ambos métodos não paramétricos, e em sequência, a abordagem paramétrica com o uso do modelo de regressão para verificar qual distribuição se adequava ao tempo de vida dos pneus e ainda o modelo de Cox para modelar o risco abordagem semi-paramétrica. Na aplicação dos métodos foi utilizado o software R por meio do pacote survival. O modelo Weibull foi mais adequado para modelar o tempo de vida dos pneus 11.00R22.
Cumprimento do protocolo clínico para atendimento de usuários de análogos de insulina em portadores de Diabetes Mellitus via judicial em Belém, Pará
Authors: Brandão Yasmin Hanna Couto , Bormann Paula Doriani dos Santos , Queiroz Auriekson Noronha , Soler Orenzio
Published: January 2021

Objetivo: Avaliar o cumprimento dos critérios estabelecidos para o atendimento via judicial de análogos de insulina para utentes portadores de Diabetes mellitus do Centro de Especialidades Médico Odontológica (CEMO), em Belém, Pará. Método: Trata-se de um estudo de caso, realizado entre agosto de 2018 e junho de 2019, utilizando-se de amostragem de conveniência, tendo como critérios de inclusão, utentes com Diabetes mellitus na faixa etária entre 50 e 59 anos, de ambos os sexos, em uso de insulinas análogas lispro (Humalog®), asparte (NovoRapid®), glulisina (Lispro®), glargina (Lantus®), detemir (Levemir®), degludeca (Tresiba®), sob demanda judicial. Resultados: Evidenciou-se o cumprimento dos critérios de inclusão; porém, não dos critérios para a manutenção e para a exclusão do programa de acesso a insulinas análogas sob demanda judicial. Conclusão: Infere-se que os critérios estabelecidos para o atendimento via judicial de análogos de insulina para utentes portadores de Diabetes mellitus no Centro de Especialidades Médico Odontológica (CEMO) do município de Belém, Pará, são parcialmente cumpridos.
Armazenamento de grãos na agricultura familiar: principais problemáticas e formas de armazenamento na região nordeste paraense
Authors: Silva Aline Oliveira da , Silva Alasse Oliveira da , Gomes Jhonatah Albuquerque , Oliveira Raylan Costa de , Silva Diocléa Almeida Seabra , Viégas Ismael de Jesus Matos
Published: January 2021

A etapa de armazenamento é tida como uma das fases mais importantes e indispensáveis dentro das etapas de pós-colheita de grãos e sementes. O armazenamento inadequado e a aparição de agentes patogênicos afetam diretamente a sanidade e durabilidade dos grãos ocasionando percas expressivas na produtividade. Diante disso, o trabalho teve como objetivo analisar as principais formas de armazenamento e embalagens utilizadas pelos agricultores familiares e as principais dificuldades enfrentadas na região nordeste paraense. Os principais grãos produzidos pelos agricultores familiares são feijão, milho, arroz e o café, cultura essas que têm grande expressão cultural e econômica, tendo em vista que todos esses compõem como base na alimentação humana e também na alimentação animal. O armazenamento nas propriedades rurais é de grande importância, visto que traz muitos benefícios ao produtor, como, comercialização com maiores lucros, visto que pode ser armazenado e vendido nos períodos de menor oferta, redução de perdas pós-colheita devido ataque de pragas, doenças e mudanças na temperatura e umidade. Apesar da prática de armazenamento ser de extrema importância, grande parte dos produtores rurais ainda utilizam técnicas rudimentares e estruturas improvisadas, que normalmente são inadequadas para o correto acondicionamento dos grãos.
Conflitos de proximidade na mineração no vale do Rio Tijucas/SC
Authors: Ravazzoli Cláudia , Scheibe Luiz Fernando
Published: January 2021

A extração de agregados para construção civil no Vale do Rio Tijucas/SC teve início por volta do ano de 1900, e causou, em tempos mais recentes, uma série de impactos sociais e ambientais fazendo emergir conflitos do tipo ‘de proximidade’. Conflitos de Proximidade é uma teoria ou metodologia desenvolvida pelo geógrafo francês Patrice Melé que caracteriza um determinado tipo de conflito social onde se valoriza os aspectos dos seus efeitos, tendo sido elaboradas quatro hipóteses de produção desses conflitos quais sejam: processo de territorialização, processo de transações sociais, construção de um espaço público intermediário e processo de atualização local da lei. Neste artigo buscamos verificar se essas hipóteses podem ser aplicadas aos conflitos da região do Vale do Rio Tijucas. A metodologia contou com revisão bibliográfica, saídas de campo e entrevistas. Concluímos que a metodologia francesa se aplica no estudo de caso brasileiro apresentado, podendo eventualmente ser utilizada em outros casos em nosso País.
Melhoria do acesso em uma Estratégia Saúde da Família em tempos de pandemia pela COVID-19: um relato de experiência
Authors: Lourenço Geovane Menezes , Rodrigues Diego Osmar , Chenek Kamila Silva , Lima Anne Karollyne de , Gustavson Jackson Paiva , Lech Anna Julia Zilli
Published: January 2021

O objetivo do presente artigo é relatar a experiência das equipes de estratégia saúde da família (ESF) de uma unidade de saúde, quanto a reorganização do fluxo do atendimento com intuito de melhorar o acesso em tempos de pandemia devido ao COVID-19. Consiste em um estudo descritivo, no qual se buscou reorganização do fluxo e do atendimento, visando proporcionar o acesso integral às pessoas no serviço de saúde, diretamente ou indiretamente, evitar número de contágio e infecção por COVID-19 entre a população, diminuir o risco de contágio aos profissionais da unidade, evitar a propagação rápida do vírus.  Percebe-se que o processo de organização do fluxo da unidade teve bons resultados como participação interprofissionais e adesão dos pacientes, a equipe conseguiu suprir as necessidades básicas dos pacientes, mantendo vínculo com os usuários priorizando as situações crônicas e descompensadas, bem como os cuidados preventivos com foco na promoção de saúde, enfatizando a prevenção da infecção pelo Sars-Cov 2.
Digital Monument Reconstruction in Architectural Studies: Synthesis of Research on the Previously Unknown Form of the Palace in Łobzów (Cracow) from the Period of the Rule of John III Sobieski
Authors: Pikulski Piotr
Published: January 2021

The former palace in Łobzów, which currently houses the Faculty of Architecture of the Cracow University of Technology, has an exceedingly rich architectural history. Since the Middle Ages, it has gone through a series of changes that have significantly altered its form each time. Thanks to modern digital reconstruction technology, it was possible to recreate all of its architectural phases in the form of 3D models on the basis of archaeological studies and the analysis of historical materials. The models were then used to reconstruct the most probable appearance of the building during the period when the Polish king John III Sobieski lived there. Determining the most probable state of the palace’s preservation during King Sobieski’s rule, which had not been investigated thus far, was possible only because of the combining of traditional research methods with modern technology.
Rebound Bursts in GABAergic Neurons of the Thalamic Reticular
Nucleus in Postnatal Mice
Authors: Wang X , Yu G , Hou X , Zhou J , Yang B , Zhang L
Published: January 2021

Whole cell patch-clamp recordings from GABAergic cells of
thalamic reticular nucleus (RTN) in thalamocortical slices made
from postnatal day 6 (P6) to 10 (P10) were used to investigate
the pattern of rebound bursts (RBs) triggered by an injection of
hyperpolarizing current into RTN cells. The number of RBs in the
RTN and the overlying Na+/K+ spikes changed in an agedependent manner. The generation of RBs depended largely on
the amplitude of the after-hyperpolarizations (AHPs). RB patterns
in response to hyperpolarizing current injection into relay cells
were markedly different from RB patterns in RTN cells with an
after-depolarization. GABAA receptor antagonist bicuculline
methiodide (BMI) changed burst firing patterns, increasing the
duration of RB and decreasing the amplitude of AHP in RTN cells.
Furthermore, local puffs of NMDA in the presence of BMI induced
RBs. K+ channel blocker 4-aminopyridine partially mimicked the
effect of BMI on AHPs. The shapes of RBs were altered by a
selective CaMKII inhibitor KN-62, but not by an inactive analog
KN-04.
Increased Expression of Erythropoietin Receptor in 1-Methyl-4-
Phenyl-1, 2, 3, 6-Tetrahydropyridine-Induced Parkinsonian Model
Authors: Wu Y , Sun X , Feng X , Liu R , Huang L , Shang Y , Liang Z , Chen J , Qiao X , Liu C , Sun S
Published: January 2021

Erythropoietin (EPO), known for its role in erythroid differentiation,
has been suggested to have a direct protective role against a
variety of neurotoxic insults. In the present study, we investigated
the expression of EPO receptor (EPOR) and the number of EPORpositive cells in three encephalic regions (ventral mesencephalon,
striatum, cortex) following lesion induced by 1-methyl-4-phenyl1,2,3,6-tetrahydropyridine (MPTP). C57BL/6 mice underwent
intraperitoneal injection of MPTP at 24 h intervals for 5 days, and
their brains were examined 1, 2, 4, 7, 14 or 21 days after the last
injection. Western blot and immunohistochemistry analysis
revealed that EPOR was dramatically up-regulated in the ventral
mesencephalon, 4 days after MPTP insult until the day 21. In
contrast, there was a baseline level of EPOR in the striatum and
cortex. At subsequent time points after MPTP injury, the levels of
EPOR in the two regions were not statistically different compared
with those in normal animals. These results suggest that the
regional specific up-regulation of EPOR at an early stage after
MPTP stimulus may represent a pro-survival mechanism against
neurotoxin injury in Parkinsonian model.
Orexins Activates Protein Kinase C-Mediated Ca2+ Signaling in
Isolated Rat Primary Sensory Neurons
Authors: Ozcan M , Ayar A , Serhatlioglu I , Alcin E , Sahin Z , Kelestimur H
Published: January 2021

Previous results have suggested that orexins causes a rise of
intracellular free calcium ([Ca2+]i) in cultured rat dorsal root
ganglion (DRG) neurons, implicating a role in nociception, but the
underlying mechanism is unknown. Hence, the aim of the present
study was to investigate whether the orexins-mediated signaling
involves the PKC pathways in these sensory neurons. Cultured
DRG neurons were loaded with 1 μmol Fura-2 AM and [Ca2+]i
responses were quantified by the changes in 340/380 ratio using
fluorescence imaging system. The orexin-1 receptor antagonist
SB-334867-A (1 µM) inhibited the calcium responses to orexin-A
and orexin-B (59.1±5.1 % vs. 200 nM orexin-A, n=8, and
67±3.8 % vs. 200 nM orexin-B, n=12, respectively). The PKC
inhibitor chelerythrine (10 and 100 µM) significantly decreased
the orexin-A (200 nM)-induced [Ca2+]i increase (59.4±4.8 %
P<0.01, n=10 and 4.9±1.6 %, P<0.01, n=9) versus response to
orexin-A). It was also found that chelerythrine dose-dependently
inhibited the [Ca2+]i response to 200 nM orexin-B. In conclusion,
our results suggest that orexins activate intracellular calcium
signaling in cultured rat sensory neurons through PKC-dependent
pathway, which may have important implications for nociceptive
modulation and pain.
Anticonvulsant Action of a New Analogue of Allopregnanolone in
Immature Rats
Authors: Mareš P , Kubová H , Kasal A
Published: January 2021

Neuroactive steroids represent potential antiepileptic drugs. We
tested a newly synthesized analogue of allopregnanolone 3αhydroxy-21ξ,22-oxido-21-homo-5α-pregnan-20-on (HOHP) against two types of pentylenetetrazol-induced seizures
(100 mg/kg s.c.) in 12- and 25-day-old rats. Ganaxolone, a neuroactive steroid in clinical trials, served as a reference drug. Pretreatment with either steroid suppressed generalized tonicclonic seizures in both age groups, their efficacy was comparable.
HOHP as well as ganaxolone were more active in 12- than in
25-day-old rats (effective doses were 40 and 60 mg/kg,
respectively). Minimal clonic seizures, which can be elicited only
in 25-day-old rats, were not influenced by any drug. Very short
duration of anticonvulsant action of HPOP demonstrated in
12-day-old animals indicates that this drug might be used only in
acute treatment in epileptology.
Integration of the Thiol Redox Status with Cytokine Response to
Physical Training in Professional Basketball Players
Authors: Zembron-Lacny A , Slowinska-Lisowska M , Ziemba A
Published: January 2021

The present study was designed to evaluate the plasma markers
of reactive oxygen species (ROS) activity and cytokines, and their
relationship with thiol redox status of basketball players during
training. Sixteen professional players of the Polish Basketball
Extraleague participated in the study. The study was performed
during the preparatory period and the play-off round. Markers of
ROS activity (lipid peroxidation TBARS, protein carbonylation PC)
and reduced glutathione (GSH) demonstrated regularity over
time, i.e. TBARS, PC and GSH were elevated at the beginning
and decreased at the end of training periods. Oxidized
glutathione (GSSG) was not affected by exercise training. Thiol
redox status (GSHtotal-2GSSG/GSSG) correlated with TBARS and
PC in both training periods. The level of interleukin-6 (IL-6) was
increased and positively correlated with thiol redox (r=0.423) in
the preparatory period, whereas tumor necrosis factor α (TNFα)
was increased and inversely correlated with thiol redox
(r=-0.509) in the play-off round. The present study showed
significant shifts in markers of ROS activity, thiol redox status and
inflammatory mediators (IL-6, TNFα) following professional sport
training as well as correlation between changes in thiol redox and
cytokine response.
High level cognitive process of high school students in solving mathematics problems based on learning style
Authors: Jamilah Irma Zahrotul , Sulaiman Raden , Wijayanti Pradnyo
Published: January 2021

Tujuan dari penelitian ini adalah untuk mendeskripsikan proses kognitif tingkat tinggi siswa SMA dalam memecahkan masalah matematika ditinjau gaya belajar visual, auditori dan kinestetik. Penelitian ini merupakan penelitian kualitatif. Subjek penelitian adalah 3 orang siswa yang diambil dari 32 siswa kelas XII-1 SMAN 15 Surabaya yang memiliki gaya belajar visual, auditori dan kinestetik serta kemampuan matematika setara. Instrumen dalam penelitian ini meliputi peneliti, tes kemampuan matematika, angket gaya belajar, tes pemecahan masalah matematika serta pedoman wawancara. Proses analisis jawaban siswa dalam memecahkan masalah matematika berdasarkan proses kognitif tingkat tinggi Anderson dan Krathwohl yang meliputi analisis, evaluasi dan mencipta serta tahapan pemecahan masalah Polya. Hasil penelitian menunjukkan bahwa siswa visual, auditori dan kinestetik melewati proses kognitif tingkat tinggi yang berbeda. Siswa kinestetik mampu melalui proses kognitif hingga pada tahap proses kognitif planning. Siswa visual dan auditori melalui proses kognitif hingga pada tahap critiquing. Siswa perlu dilatih menyelesaikan masalah matematika dengan cara yang beragam.
Functional Gradient Metallic Biomaterials: Techniques, Current Scenery, and Future Prospects in the Biomedical Field
Authors: Shi Hongyuan , Zhou Peng , Li Jie , Liu Chaozong , Wang Liqiang
Published: January 2021

Functional gradient materials (FGMs), as a modern group of materials, can provide multiple functions and are able to well mimic the hierarchical and gradient structure of natural systems. Because biomedical implants usually substitute the bone tissues and bone is an organic, natural FGM material, it seems quite reasonable to use the FGM concept in these applications. These FGMs have numerous advantages, including the ability to tailor the desired mechanical and biological response by producing various gradations, such as composition, porosity, and size; mitigating some limitations, such as stress-shielding effects; improving osseointegration; and enhancing electrochemical behavior and wear resistance. Although these are beneficial aspects, there is still a notable lack of comprehensive guidelines and standards. This paper aims to comprehensively review the current scenery of FGM metallic materials in the biomedical field, specifically its dental and orthopedic applications. It also introduces various processing methods, especially additive manufacturing methods that have a substantial impact on FGM production, mentioning its prospects and how FGMs can change the direction of both industry and biomedicine. Any improvement in FGM knowledge and technology can lead to big steps toward its industrialization and most notably for much better implant designs with more biocompatibility and similarity to natural tissues that enhance the quality of life for human beings.
Mammalian SWI/SNF Chromatin Remodeling Complexes in Embryonic Stem Cells: Regulating the Balance Between Pluripotency and Differentiation
Authors: Ye Ying , Chen Xi , Zhang Wensheng
Published: January 2021

The unique capability of embryonic stem cells (ESCs) to maintain and adjust the equilibrium between self-renewal and multi-lineage cellular differentiation contributes indispensably to the integrity of all developmental processes, leading to the advent of an organism in its adult form. The ESC fate decision to favor self-renewal or differentiation into specific cellular lineages largely depends on transcriptome modulations through gene expression regulations. Chromatin remodeling complexes play instrumental roles to promote chromatin structural changes resulting in gene expression changes that are key to the ESC fate choices governing the equilibrium between pluripotency and differentiation. BAF (Brg/Brahma-associated factors) or mammalian SWI/SNF complexes employ energy generated by ATP hydrolysis to change chromatin states, thereby governing the accessibility of transcriptional regulators that ultimately affect transcriptome and cell fate. Interestingly, the requirement of BAF complex in self-renewal and differentiation of ESCs has been recently shown by genetic studies through gene expression modulations of various BAF components in ESCs, although the precise molecular mechanisms by which BAF complex influences ESC fate choice remain largely underexplored. This review surveys these recent progresses of BAF complex on ESC functions, with a focus on its role of conditioning the pluripotency and differentiation balance of ESCs. A discussion of the mechanistic bases underlying the genetic requirements for BAF in ESC biology as well as the outcomes of its interplays with key transcription factors or other chromatin remodelers in ESCs will be highlighted.
Sophisticated Gene Regulation for a Complex Physiological System: The Role of Non-coding RNAs in Photoreceptor Cells
Authors: Carrella Sabrina , Banfi Sandro , Karali Marianthi
Published: January 2021

Photoreceptors (PRs) are specialized neuroepithelial cells of the retina responsible for sensory transduction of light stimuli. In the highly structured vertebrate retina, PRs have a highly polarized modular structure to accommodate the demanding processes of phototransduction and the visual cycle. Because of their function, PRs are exposed to continuous cellular stress. PRs are therefore under pressure to maintain their function in defiance of constant environmental perturbation, besides being part of a highly sophisticated developmental process. All this translates into the need for tightly regulated and responsive molecular mechanisms that can reinforce transcriptional programs. It is commonly accepted that regulatory non-coding RNAs (ncRNAs), and in particular microRNAs (miRNAs), are not only involved but indeed central in conferring robustness and accuracy to developmental and physiological processes. Here we integrate recent findings on the role of regulatory ncRNAs (e.g., miRNAs, lncRNAs, circular RNAs, and antisense RNAs), and of their contribution to PR pathophysiology. We also outline the therapeutic implications of translational studies that harness ncRNAs to prevent PR degeneration and promote their survival and function.
Letrozole Versus Clomiphene Citrate and Natural Cycle: Endometrial Receptivity During Implantation Window in Women With Polycystic Ovary Syndrome
Authors: Wang Li , Lv Shulan , Li Fen , Bai E. , Yang Xiaofeng
Published: January 2021

Objective

Numerous studies have reported on ovulation and pregnancy rates in patients with polycystic ovary syndrome (PCOS). However, relevant data on endometrial receptivity are limited. This study was conducted to compare endometrial receptivity during implantation windows among letrozole (LE), clomiphene citrate (CC), and natural cycle, and to assess the predictive value for pregnancy of observed indicators.

Methods

This randomized controlled trial study enrolled 270 patients with PCOS. Patients were given LE (n=90) at a dose of 2.5mg/day or CC (n=90) at a dose of 50 mg/day on cycle days 5–9 for ovulation induction. Patients in the natural cycle group (n=90) did not receive any drug for ovulation induction. Endometrial ultrasonic parameters, integrin αvβ3, and vascular endothelial growth factor (VEGF) concentrations in uterine secretion were detected during the implantation window. The endometrial receptivity, ovulation rate, pregnancy rates, and predictive value of observed indicators for pregnancy were analyzed.

Results

The successful ovulation rate did not differ between the LE group and CC group (P>0.05). Endometrial ultrasonic parameters [endometrial thickness (ET), endometrial volume (EV), vascularization index (VI), flow index (FI), vascularization flow index (VFI)], integrin αvβ3, and VEGF concentrations in uterine fluid were significantly higher in the LE group compared with the CC group and natural cycle group (P<0.05). The clinical pregnancy and ongoing pregnancy rates of the LE group were significantly higher than in the CC group (P<0.05). Endometrial ultrasonic parameters (VI, FI, and VFI), integrin αvβ3, and VEGF concentrations in uterine fluid of all pregnancy groups were significantly higher compared with the no pregnancy group (P<0.05), and the above parameters in ongoing pregnancy were significantly higher than in biochemical pregnancy (P<0.05). The endometrial FI during the implantation window had the highest predictive value for pregnancy (AUC=0.889). The integrin αvβ3 in uterine fluid had better predictive value (AUC=0.876) than VEGF.

Conclusions

Endometrial receptivity during the implantation window of LE is superior to CC in PCOS women, which may be related to higher clinical pregnancy and ongoing pregnancy rates. Endometrial FI examined by 3-D power Doppler, and integrin αvβ3 in uterine secretion during the implantation window, could be preferable non-invasive predictor markers for pregnancy.

Clinical Trial Registration

www.chictr.org.cn, ChiCTR1900023423.
Epigenetic Regulation Associated With Sirtuin 1 in Complications of Diabetes Mellitus
Authors: Wang(a) Jie , Wang Shudong , Wang(b) Jie , Xiao Mengjie , Guo Yuanfang , Tang Yufeng , Zhang Jingjing , Gu Junlian
Published: January 2021

Diabetes mellitus (DM) has been one of the largest health concerns of the 21st century due to the serious complications associated with the disease. Therefore, it is essential to investigate the pathogenesis of DM and develop novel strategies to reduce the burden of diabetic complications. Sirtuin 1 (SIRT1), a nicotinamide adenosine dinucleotide (NAD+)-dependent deacetylase, has been reported to not only deacetylate histones to modulate chromatin function but also deacetylate numerous transcription factors to regulate the expression of target genes, both positively and negatively. SIRT1 also plays a crucial role in regulating histone and DNA methylation through the recruitment of other nuclear enzymes to the chromatin. Furthermore, SIRT1 has been verified as a direct target of many microRNAs (miRNAs). Recently, numerous studies have explored the key roles of SIRT1 and other related epigenetic mechanisms in diabetic complications. Thus, this review aims to present a summary of the rapidly growing field of epigenetic regulatory mechanisms, as well as the epigenetic influence of SIRT1 on the development and progression of diabetic complications, including cardiomyopathy, nephropathy, and retinopathy.
Metal-Organic Framework MOF-76(Nd): Synthesis, Characterization, and Study of Hydrogen Storage and Humidity Sensing
Authors: Garg Akash , Almáši Miroslav , Rattan Paul Devina , Poonia Ekta , Luthra J. R. , Sharma Anshu
Published: January 2021

The nanoporous metal-organic framework (MOF), MOF-76(Nd) [neodymium (III) benzene-1,3,5-tricarboxylate], has been synthesized, characterized, and tested for hydrogen storage and humidity sensing applications. These synthesized MOFs were characterized using scanning and transmission electron microscopy techniques. Thermal analysis revealed that, after the dehydration process, the compound showed high thermal stability up to 500°C. Hydrogen adsorption/desorption measurements of MOF-76(Nd) were performed at 77K and 20 bar and the material was further used for the humidity measurement at room temperature.