bluuebunny/crossref_metadata_2025 huggingface dataset:
Dissolution Behavior of Lime with Different Properties into Converter Slag
Authors: Zhang Mengxu , Li Jianli , Jia Cao , Yu Yue
Published: September 2023

China’s 2022 crude steel production soared to an impressive 1.018 billion tons, and steel slag constituted approximately 10% to 15% of this massive output. However, a notable hindrance to the comprehensive utilization of steel slag arises from the fact that it contains 10% to 20% of free calcium oxide (f-CaO), resulting in volume instability. To address this challenge, our study delved into the dynamic transformation of the interface between lime and slag, as well as the fluctuations in the dissolution rate of lime. An Electron Probe Micro Analyzer, equipped with an energy-dispersive spectrometer, was employed for the analysis. Our findings revealed that the configuration of the reaction interface between quicklime and slag underwent alterations throughout various phases of converter smelting. At a temperature of 1400 °C, several significant transformations occurred, including the formation of a CaO-FeO solid solution, (Ca, Mg, Fe) olivine, and low-melting point (Ca, Mg) silicate minerals. With the gradual reduction in FeO content, a robust and high-melting 2CaO·SiO2 layer emerged, generated through the interaction between CaO and (Ca, Mg, Fe) olivine. Furthermore, for lime with a particle size of 20 mm and a calcination rate of 0%, the thickest layer of 2CaO·SiO2 was observed after 120 s of dissolution in slag A2 at 1400 °C. Overall, the dissolution rates of lime with different particle sizes in slag A1 to A4 showed a gradual increase. On the other hand, the dissolution rates of lime with different calcination rates in slag A1 to A4 exhibited an initial increase, followed by a decrease, and then another increase. The formation of a high-melting point and continuous dense 2CaO·SiO2 layer during the dissolution process hindered the mass transfer between lime and slag.
Development and Performance Evaluation of an Asphalt Regenerant Derived from Waste Engine Oil Residue
Authors: Li Rukai , Pang Zhansheng , Ling Tianqing , Wang Tengfei
Published: September 2023

This study assessed the fundamental physical properties and chemical composition of three specific waste engine oil residue (WEORs) asphalt regenerants. Through dynamic shear rheometer and rolling thin-film oven tests, the performance of aged asphalt was evaluated using three key indicators. Thin-layer chromatography investigations probed the WEOR-induced changes in the aging asphalt components, leading to the creation of two novel asphalt regenerants, WEOR-H and WEOR-G. WEOR-G was developed from WEOR-1, liquid rubber, ultraviolet absorber, light shielding agent, and antioxidant, while WEOR-H was formulated from WEOR-2, aromatic oil, and liquid rubber. The study employed differential scanning calorimetry and conventional laboratory tests to analyze the road performance attributes of Ingevity J type regenerant (J), WEOR-G, and WEOR-H. The results indicated that WEORs increase the saturate and aromatic content in asphalt and partially replenish the missing lightweight components of aged asphalt, moderately improving the three key indicators, though the regenerative effect is restricted. Achieving a full restoration of component proportions within aged asphalt to their initial levels proved unattainable, and direct application of any of the three WEORs as asphalt regenerants is impractical. WEOR-H and WEOR-G demonstrated potential in enhancing aged asphalt binder road performance, outpacing three other WEORs. At a 14% dosage, WEOR-G and WEOR-H could increase the 10 °C ductility to 23.5 and 21.4 cm, respectively, effectively counterbalancing the insufficient ability of WEOR-1 and WEOR-2 to restore the low-temperature performance of aged asphalt. Among the regenerants, WEOR-G, possessing superior regenerative effects, the lowest glass transition temperature, and optimal low-temperature deformation resistance, emerged as the most efficacious. This inquiry furnishes vital data support for future applications of WEOR-G asphalt regenerant.
In Situ Observation of Micro-Patterned Elastomeric Surfaces: The Formation of the Area of Real Contact and the Influence on Its Friction and Deformation Behaviour
Authors: Hausberger Andreas , Pecora Marina , Favier Damien , Rossegger Elisabeth , Tockner Martin , Ules Thomas , Haselmann Matthias , Schlögl Sandra , Gauthier Christian
Published: September 2023

Structured surfaces, which are the basis of the lotus blossom effect, have great potential to serve/operate as functionalised surfaces, i.e., surfaces with specific and/or adjustable properties. In the present study, the aim is to use micro-structured elastomeric surfaces to specifically influence the friction and deformation behaviours on the basis of the shape and arrangement of the structures. Thiol-acrylate-based photopolymers patterned via nanoimprint lithography were investigated by using an in situ tribological measurement set-up. A clear influence of the different structures on the surface’s friction behaviour could be shown, and, furthermore, this could be brought into relation with the real area of contact. This finding provides an important contribution to further development steps, namely, to give the structures switchable properties in order to enable the control of friction properties in a targeted manner.
Characterization of Hot-Applied Joint Sealants and Their Components in Terms of Their Chemical Composition and Basic Performance Properties
Authors: Stępień Justyna , Remišová Eva
Published: September 2023

Hot- and cold-applied joint sealants are materials commonly used for the surface repairs of the upper layers of asphalt or concrete road surfaces. Our investigations covered six hot-applied joint sealants, classified as the high-extension type N1 (elastic) or low-extension type N2 (normal), in accordance with the standard EN 14188-1; the sealants were obtained commercially from four European manufacturers. The present paper focuses on the characterization of the consistency of the joint sealants, the bituminous binders that contain them, and the characterization of their insoluble components. Additional testing methods included an FTIR analysis of the sealants and the extracted binders, as well as SEM, EDS, and sieving analyses of the insoluble material. Joint sealants are complex formulations and include a broad range of base asphalt binders and other components. Their compositions may vary widely, while still fulfilling the performance specifications. Through the extraction of the solvents and the separation of the crack sealants, it was found that radically different compositions of crack sealants resulted in the comparatively similar performance of the tested material. The EDS and FTIR analysis methods provided insights into the composition of the crack sealants and the types of mineral materials used.
Fully Physically Crosslinked Conductive Hydrogel with Ultrastretchability, Transparency, and Self-Healing Properties for Strain Sensors
Authors: Ji Feng , Shang Pengbo , Lai Yingkai , Wang Jinmei , Zhang Guangcai , Lin Dengchao , Xu Jing , Cai Daniu , Qin Zhihui
Published: September 2023

Currently, conductive hydrogels have received great attention as flexible strain sensors. However, the preparation of such sensors with integrated stretchability, transparency, and self-healing properties into one gel through a simple method still remains a huge challenge. Here, a fully physically crosslinked double network hydrogel was developed based on poly(hydroxyethyl acrylamide) (PHEAA) and κ-carrageenan (Car). The driving forces for physical gelation were hydrogen bonds, ion bonding, and electrostatic interactions. The resultant PHEAA-Car hydrogel displayed stretchability (1145%) and optical transparency (92%). Meanwhile, the PHEAA-Car hydrogel exhibited a self-healing property at 25 °C. Additionally, the PHEAA-Car hydrogel-based strain sensor could monitor different joint movements. Based on the above functions, the PHEAA-Car hydrogel can be applied in flexible strain sensors.
Performance of Osprey Optimization Algorithm for Solving Economic Load Dispatch Problem
Authors: Ismaeel Alaa A. K. , Houssein Essam H. , Khafaga Doaa Sami , Abdullah Aldakheel Eman , AbdElrazek Ahmed S. , Said Mokhtar
Published: September 2023

The osprey optimization algorithm (OOA) is a new metaheuristic motivated by the strategy of hunting fish in seas. In this study, the OOA is applied to solve one of the main items in a power system called economic load dispatch (ELD). The ELD has two types. The first type takes into consideration the minimization of the cost of fuel consumption, this type is called ELD. The second type takes into consideration the cost of fuel consumption and the cost of emission, this type is called combined emission and economic dispatch (CEED). The performance of the OOA is compared against several techniques to evaluate its reliability. These methods include elephant herding optimization (EHO), the rime-ice algorithm (RIME), the tunicate swarm algorithm (TSA), and the slime mould algorithm (SMA) for the same case study. Also, the OOA is compared with other techniques in the literature, such as an artificial bee colony (ABO), the sine cosine algorithm (SCA), the moth search algorithm (MSA), the chimp optimization algorithm (ChOA), and monarch butterfly optimization (MBO). Power mismatch is the main item used in the evaluation of the OOA with all of these methods. There are six cases used in this work: 6 units for the ELD problem at three different loads, and 6 units for the CEED problem at three different loads. Evaluation of the techniques was performed for 30 various runs based on measuring the standard deviation, minimum fitness function, and maximum mean values. The superiority of the OOA is achieved according to the obtained results for the ELD and CEED compared to all competitor algorithms.
High-Quality Reversible Data Hiding Based on Multi-Embedding for Binary Images
Authors: Li Xiang , Li Xiaolong , Xiao Mengyao , Zhao Yao , Cho Hsunfang
Published: September 2023

Unlike histogram-based reversible data hiding (RDH), the general distortion-based framework considers pixel-by-pixel distortions, which is a new research direction in RDH. The advantage of the general distortion-based RDH method is that it can enhance the visual quality of the marked image by embedding data into visually insensitive regions (e.g., edges and textures). In this paper, following this direction, a high-capacity RDH approach based on multi-embedding is proposed. The cover image is decoupled to select the embedding sequence that can better utilize texture pixels and reduce the size of the reconstruction information, and a multi-embedding strategy is proposed to embed the secret data along with the reconstruction information by matrix embedding. The experimental results demonstrate that the proposed method provides a superior visual quality and higher embedding capacity than some state-of-the-art RDH works for binary images. With an embedding capacity of 1000 bits, the proposed method achieves an average PSNR of 49.45 dB and an average SSIM of 0.9705 on the test images. This marks an improvement of 1.1 dB in PSNR and 0.0242 in SSIM compared to the latest state-of-the-art RDH method.
Hybrid Modeling of Anxiety Propagation in Response to Threat Stimuli Flow
Authors: Sakalauskas Leonidas , Denisov Vitalij , Dirzyte Aiste
Published: September 2023

Previous studies have demonstrated that the rates of anxiety have been constantly increasing worldwide in recent years. To understand this phenomenon, based on the complemented cognitive model TVAPA of anxiety, the hybrid method of modeling and simulating the dynamics of anxiety in the population is proposed. The suggested method combines agent-based modeling, dynamic systems modeling with differential equations, and machine learning methods. The four-level STAI methodology is applied to assess anxiety in the proposed models. Sentiment analysis of social media content is used to identify the parameters of triggering stimuli flow. The proposed models were implemented and verified using open access data sets. Created models are characterized by simplicity, and the parameters used in them have a clear socio-informational meaning. The developed models can be calibrated by applying statistical methods according to indicators of anxiety measured at discrete sets of time intervals by associating them with parameters of the threat stimuli flow taken from statistical data and/or Internet content tracking data.
Damage Metrics for Void Detection in Adhesive Single-Lap Joints
Authors: Tenreiro António Francisco G. , Lopes António M. , da Silva Lucas F. M.
Published: September 2023

Structural Health Monitoring (SHM) techniques, such as Electromechanical Impedance Spectroscopy (EMIS), aim to continuously monitor structures for defects, thus avoiding the need for regular maintenance. While attention has been given to the application of EMIS in the automatic detection of damage in metallic and composite components, integrity monitoring of structural adhesive joints has been comparatively neglected. This paper investigated the use of damage metrics with electrical impedance measurements to detect defects in Single-Lap Joints (SLJs) bonded with a modified epoxy adhesive. Traditional metrics using statistical and distance-based concepts, such as the Root-Mean-Squared Deviation, RMSD, or the Correlation Coefficient, CC, are addressed at detecting voids in the adhesive layer and are applied to five different spectral frequency ranges. Furthermore, new damage metrics have been developed, such as the Average Canberra Distance, ACD, which enables a reduction of possible outliers in damage detection, or the complex Root-Mean-Squared Deviation, cRMSD, which allows for the use of both the real and imaginary components of the impedance, enabling better damage detection in structural adhesive joints. Overall, damage detection is achieved, and for certain spectral conditions, differentiation between certain damage sizes, using specific metrics, such as the RMSD or cRMSD, may be possible. Overall, the RMSD or cRMSD values from damaged SLJs tend to be double the metric values from undamaged joints.
The Antiviral Potential of Algal Lectins
Authors: Alvarez Christian , Félix Carina , Lemos Marco
Published: September 2023

Algae have emerged as fascinating subjects of study due to their vast potential as sources of valuable metabolites with diverse biotechnological applications, including their use as fertilizers, feed, food, and even pharmaceutical precursors. Among the numerous compounds found in algae, lectins have garnered special attention for their unique structures and carbohydrate specificities, distinguishing them from lectins derived from other sources. Here, a comprehensive overview of the latest scientific and technological advancements in the realm of algal lectins with a particular focus on their antiviral properties is provided. These lectins have displayed remarkable effectiveness against a wide range of viruses, thereby holding great promise for various antiviral applications. It is worth noting that several alga species have already been successfully commercialized for their antiviral potential. However, the discovery of a diverse array of lectins with potent antiviral capabilities suggests that the field holds immense untapped potential for further expansion. In conclusion, algae stand as a valuable and versatile resource, and their lectins offer an exciting avenue for developing novel antiviral agents, which may lead to the development of cutting-edge antiviral therapies.
Angiotensin-I-Converting Enzyme (ACE)-Inhibitory Peptides from the Collagens of Monkfish (Lophius litulon) Swim Bladders: Isolation, Characterization, Molecular Docking Analysis and Activity Evaluation
Authors: Hu Yu-Dong , Xi Qing-Hao , Kong Jing , Zhao Yu-Qin , Chi Chang-Feng , Wang Bin
Published: September 2023

The objective of this study was to isolate and characterize collagen and angiotensin-I-converting enzyme (ACE)-inhibitory (ACEi) peptides from the swim bladders of monkfish (Lophius litulon). Therefore, acid-soluble collagen (ASC-M) and pepsin-soluble collagen (PSC-M) with yields of 4.27 ± 0.22% and 9.54 ± 0.51%, respectively, were extracted from monkfish swim bladders using acid and enzyme methods. The ASC-M and PSC-M contained Gly (325.2 and 314.9 residues/1000 residues, respectively) as the major amino acid, but they had low imino acid content (192.5 and 188.6 residues/1000 residues, respectively) in comparison with collagen from calf skins (CSC) (216.6 residues/1000 residues). The sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) patterns and ultraviolet (UV) absorption spectrums of ASC-M and PSC-M illustrated that they were mainly composed of type I collagen. Subsequently, three ACEi peptides were isolated from a PSC-M hydrolysate prepared via a double-enzyme system (alcalase + neutrase) and identified as SEGPK (MHP6), FDGPY (MHP7) and SPGPW (MHP9), with molecular weights of 516.5, 597.6 and 542.6 Da, respectively. SEGPK, FDGPY and SPGPW displayed remarkable anti-ACE activity, with IC50 values of 0.63, 0.94 and 0.71 mg/mL, respectively. Additionally, a molecular docking assay demonstrated that the affinities of SEGPK, FDGPY and SPGPW with ACE were −7.3, −10.9 and −9.4 kcal/mol, respectively. The remarkable ACEi activity of SEGPK, FDGPY and SPGPW was due to their connection with the active pockets and/or sites of ACE via hydrogen bonding, hydrophobic interaction and electrostatic force. Moreover, SEGPK, FDGPY and SPGPW could protect HUVECs by controlling levels of nitric oxide (NO) and endothelin-1 (ET-1). Therefore, this work provides an effective means for the preparation of collagens and novel ACEi peptides from monkfish swim bladders, and the prepared ACEi peptides, including SEGPK, FDGPY and SPGPW, could serve as natural functional components in the development of health care products to control hypertension.
Bird’s Eye View on Lattice Structures: Design Issues and Applications for Best Practices in Mechanical Design
Authors: Ahmad Abas , Belluomo Luca , Bici Michele , Campana Francesca
Published: September 2023

Lattice structures for engineering applications are patterns of unit cells designed to make a larger functional structure. Research on lattice structures ranges in many fields, from mechanical characterization and cell and pattern designs in respect of their applications, to the manufacturing process and its final shape control. From the manufacturing point of view, some kinds of lattice structures can be infeasible when approached with traditional manufacturing methods. It may offer an inevitable limitation of their adoption. However, advancements in Additive Manufacturing (AM) have solved this manufacturing issue to a great extent, allowing to obtain major complexity of the cells that can be achieved. The topology, shape of the unit cell, and the characteristics of its replication pattern allow us to obtain many kinds of structures in respect of the different engineering requirements and manufacturing constraints. Nevertheless, the necessity of new or dedicated CAD-CAE approaches arises to manage the domains of multiscale modeling. These are some of the advantages and disadvantages that may arise while approaching the design of a component using lattice structures. The aim of this paper is to provide an overview that integrates the most recent applications of lattice structures with their related design and manufacturing issues so that, from a practical design point of view, any state-of-the-art improvements may be established in respect of the related field of applications. In this article, engineers and researchers may find a practical summary of the capabilities and processes of lattice structures that are currently available from a design and development point of view.
Studying the Effect of Cr and Si on the High-Temperature Oxidation-Resistance Mechanism of Hot Stamping Steel
Authors: Wu Yanxin , Zhang Qi , Zhu Rong , Wang Mai , Jiang Haitao , Mi Zhenli
Published: September 2023

The surface of hot stamping steel is severely oxidized during heating, holding, and transfer from the heating furnace to the stamping die in the production of traditional automotive parts. Coating-free hot stamping steel with Cr and Si elements exhibits excellent oxidation resistance during hot stamping without the protection of a surface coating. This paper investigates the oxidation behavior of three types of hot stamping steel at 800–1200 °C. The results show that although Cr-Si hot stamping steel performs excellently short-term (≤7.5 min) for oxidation resistance, its long-term (≥15 min) or high-temperature (≥1100 °C) oxidation resistance is much lower than that of the conventional hot stamping steel 22MnB5, affecting the production and surface quality control of the new coating-free Cr-Si hot stamping steel. By analyzing the oxidation kinetics and characterizing the structure of oxide layers in hot stamping steel, it was found that the structural change in the Cr and Si element enrichment layer between the oxide scale and the substrate varied in oxidation performance at different temperatures. When the oxidation temperature was below 1000 °C, the solid Cr and Si enrichment layer acted as a barrier to prevent the diffusion of Fe ions. When the oxidation temperature exceeded 1100 °C, the molten Cr and Si enrichment layer effectively adapted to the substrate and avoided blistering. Meanwhile, Fe2SiO4 penetrated the Fe oxide layer along the grain boundary and became a rapidly diffusing channel of Fe ions, contributing to a significant increase in the oxidation rate.
Research of Three-High Screw Rolling of Aluminum Billets with Copper Inserts at Different Rolls Feed Angles
Authors: Skripalenko Mikhail M. , Rogachev Stanislav O. , Bazhenov Viacheslav E. , Romantsev Boris A. , Skripalenko Mikhail N. , Karpov Boris V. , Titov Andrey Yu. , Koltygin Andrey V. , Danilin Andrei V.
Published: September 2023

Three-high screw-rolling of aluminum alloy ingots was carried out at 12- and 20-degrees rolls feed angles. The ingots had two copper, cylindrical inserts oriented along the ingot axis: the center of one insert coincided with the ingot’s center, and the center of the other insert coincided with the ingot’s half-radius. The effect of the rolls feed angle value on the aluminum and copper microstructure as well as the hardness formation was established. X-ray study and three-dimensional modeling allowed the copper insert twisting angle to be estimated along the rolled billet axis. It also allowed detection of the number of breaks of the insert located in the ingot’s center and the insert located in the ingot’s half-radius depending on the rolls feed angle value.
Effect of Ultrasonic Surface Rolling on the Fretting Wear Property of 7075 Aluminum Alloy
Authors: Xiong Qiwen , Zhang Po , Zhai Wenzheng , Luo Xiaoshuang , Cai Zhaobing , Zheng Feilong , Gu Le
Published: September 2023

This paper investigates the effect of ultrasonic surface rolling (USR) on the fretting wear properties of the 7075 aluminum alloy. A white light interferometer, Vickers hardness tester, and X-ray diffractometer were employed to comparatively analyze the variations in surface roughness, hardness. and grain size before and after the USR treatment. The fretting tests were carried out under oil lubricated and dry fretting conditions, using a ball-on-flat contact tangential fretting tester. The worn surface morphology, wear debris, and chemical composition were analyzed using an optical microscope (OM), a scanning electron microscope (SEM), and X-ray photoelectron spectroscopy (XPS), etc. The results revealed that after USR treatment, the surface roughness was reduced by 90%, the hardness was increased by 13HV, and the grains were refined. Moreover, the wear was reduced under oil lubrication conditions but increased under dry fretting conditions. It can be concluded that the microstructure and mechanical properties of the 7075 aluminum alloy surface can be enhanced by the USR treatment. The improved fretting performance in oil should be attributed to the increased surface hardness, which helps reduce abrasive wear and plastic deformation. However, under dry fretting conditions, the wear was increased due to the presence of hard debris particles that peel off from the USR-treated surface, leading to aggravated abrasion.
Lactobacillus johnsonii Attenuates Liver Steatosis and Bile Acid Dysregulation in Parenteral Nutrition-Fed Rats
Authors: Xu Juan , Zhou Yongchang , Cheng Siyang , Zhao Yuling , Yan Junkai , Wang Ying , Cai Wei , Jiang Lu
Published: September 2023

Parenteral nutrition (PN), a vital therapy for patients with intestinal failure, can lead to the development of parenteral nutrition-associated liver disease (PNALD). In this study, we aimed to investigate the role of Lactobacillus johnsonii (L. johnsonii) in a rat model of PNALD. Total parenteral nutrition (TPN)-fed rats were used to assess the role of L. johnsonii in liver steatosis, bile acid metabolism, gut microbiota, and hepatocyte apoptosis. We observed a depletion of L. johnsonii that was negatively correlated with the accumulation of glycochenodeoxycholic acid (GCDCA), a known apoptosis inducer, in rats subjected to TPN. L. johnsonii attenuated TPN-induced liver steatosis by inhibiting fatty acid synthesis and promoting fatty acid oxidation. TPN resulted in a decrease in bile acid synthesis and biliary bile secretion, which were partially restored by L. johnsonii treatment. The gut microbial profile revealed depletion of pathogenic bacteria in L. johnsonii-treated rats. L. johnsonii treatment reduced both hepatic GCDCA levels and hepatocyte apoptosis compared with the TPN group. In vitro, L. johnsonii treatment inhibited GCDCA-induced hepatocyte apoptosis via its bile salt hydrolase (BSH) activity. Our findings suggest that L. johnsonii protects against liver steatosis, bile acid dysregulation, and hepatocyte apoptosis in TPN-fed rats.
Particle Separation in a Microchannel with a T-Shaped Cross-Section Using Co-Flow of Newtonian and Viscoelastic Fluids
Authors: Song Jinhyeuk , Jang Jaekyeong , Kim Taehoon , Cho Younghak
Published: September 2023

In this study, we investigated the particle separation phenomenon in a microchannel with a T-shaped cross-section, a unique design detailed in our previous study. Utilizing a co-flow system within this T-shaped microchannel, we examined two types of flow configuration: one where a Newtonian fluid served as the inner fluid and a viscoelastic fluid as the outer fluid (Newtonian/viscoelastic), and another where both the inner and outer fluids were Newtonian fluids (Newtonian/Newtonian). We introduced a mixture of three differently sized particles into the microchannel through the outer fluid and observed that the co-flow of Newtonian/viscoelastic fluids effectively separated particles based on their size compared with Newtonian/Newtonian fluids. In this context, we evaluated and compared the particle separation efficiency, recovery rate, and enrichment factor across both co-flow configurations. The Newtonian/viscoelastic co-flow system demonstrated a superior efficiency and recovery ratio when compared with the Newtonian/Newtonian system. Additionally, we assessed the influence of the flow rate ratio between the inner and outer fluids on particle separation within each co-flow system. Our results indicated that increasing the flow rate ratio enhanced the separation efficiency, particularly in the Newtonian/viscoelastic co-flow configuration. Consequently, this study substantiates the potential of utilizing a Newtonian/viscoelastic co-flow system in a T-shaped straight microchannel for the simultaneous separation of three differently sized particles.
Choice of the Miniature Inertial Optomechanical Sensor Geometric Parameters with the Help of Their Mechanical Characteristics Modelling
Authors: Kumanchik Lee , Rezinkina Marina , Braxmaier Claus
Published: September 2023

In this paper, the mechanical characteristics of a miniature optomechanical accelerometer, similar to those proposed for a wide range of applications, have been investigated. With the help of numerical modelling, characteristics such as eigenfrequencies, quality factor, displacement magnitude, normalized translations, normalized rotations versus eigenfrequencies, as well as spatial distributions of the azimuthal and axial displacements and stored energy density in a wide frequency range starting from the stationary case have been obtained. Dependencies of the main mechanical characteristics versus the minimal and maximal system dimensions have been plotted. Geometries of the optomechanical accelerometers with micron size parts providing the low and the high first eigenfrequencies are presented. It is shown that via the choice of the geometrical parameters, the minimal measured acceleration level can be raised substantially.
Editorial for the Special Issue on Micro/Nano-Structure Based Optoelectronics and Photonics Devices
Authors: Wu Zhiyong , Zhu Lu , Xu Zhengji
Published: September 2023

In the ever-evolving fields of optoelectronics and photonics, the introduction of carefully designed micro-/nanostructures enables personalized customization of the electrical and optical properties of optoelectronic and photonic devices [...]
Delineating Conformational Variability in Small Protein Structures Using Combinatorial Refinement Strategies
Authors: Kelly Deborah F. , Jonaid G M , Kaylor Liam , Solares Maria J. , Berry Samantha , DiCecco Liza-Anastasia , Dearnaley William , Casasanta Michael
Published: September 2023

As small protein assemblies and even small proteins are becoming more amenable to cryo-Electron Microscopy (EM) structural studies, it is important to consider the complementary dynamic information present in the data. Current computational strategies are limited in their ability to resolve minute differences among low molecular weight entities. Here, we demonstrate a new combinatorial approach to delineate flexible conformations among small proteins using real-space refinement applications. We performed a meta-analysis of structural data for the SARS CoV-2 Nucleocapsid (N) protein using a combination of rigid-body refinement and simulated annealing methods. For the N protein monomer, we determined three new flexible conformers with good stereochemistry and quantitative comparisons provided new evidence of their dynamic properties. A similar analysis performed for the N protein dimer showed only minor structural differences among the flexible models. These results suggested a more stable view of the N protein dimer than the monomer structure. Taken together, the new computational strategies can delineate conformational changes in low molecular weight proteins that may go unnoticed by conventional assessments. The results also suggest that small proteins may be further stabilized for structural studies through the use of solution components that limit the movement of external flexible regions.
First Report and Characterization of the mcr-1 Positive Multidrug-Resistant Escherichia coli Strain Isolated from Pigs in Croatia
Authors: Kompes Gordan , Duvnjak Sanja , Reil Irena , Hendriksen Rene S. , Sørensen Lauge Holm , Zdelar-Tuk Maja , Habrun Boris , Cvetnić Luka , Bagarić Antonela , Špičić Silvio
Published: September 2023

The emergence and rapid spread of the plasmid-mediated colistin-resistant mcr-1 gene introduced a serious threat to public health. In 2021, a multi-drug resistant, mcr-1 positive Escherichia coli EC1945 strain, was isolated from pig caecal content in Croatia. Antimicrobial susceptibility testing and whole genome sequencing were performed. Bioinformatics tools were used to determine the presence of resistance genes, plasmid Inc groups, serotype, sequence type, virulence factors, and plasmid reconstruction. The isolated strain showed phenotypic and genotypic resistance to nine antimicrobial classes. It was resistant to colistin, gentamicin, ampicillin, cefepime, cefotaxime, ceftazidime, sulfamethoxazole, chloramphenicol, nalidixic acid, and ciprofloxacin. Antimicrobial resistance genes included mcr-1, blaTEM-1B, blaCTX-M-1, aac(3)-IId, aph(3’)-Ia, aadA5, sul2, catA1, gyrA (S83L, D87N), and parC (A56T, S80I). The mcr-1 gene was located within the conjugative IncX4 plasmid. IncI1, IncFIB, and IncFII plasmids were also detected. The isolate also harbored 14 virulence genes and was classified as ST744 and O101:H10. ST744 is a member of the ST10 group which includes commensal, extraintestinal pathogenic E. coli isolates that play a crucial role as a reservoir of genes. Further efforts are needed to identify mcr-1-carrying E. coli isolates in Croatia, especially in food-producing animals to identify such gene reservoirs.
Incidence and Virulence Factor Profiling of Vibrio Species: A Study on Hospital and Community Wastewater Effluents
Authors: Mavhungu Mashudu , Digban Tennison O. , Nwodo Uchechukwu U.
Published: September 2023

This study aimed to determine the incidence and virulence factor profiling of Vibrio species from hospital wastewater (HWW) and community wastewater effluents. Wastewater samples from selected sites were collected, processed, and analysed presumptively by the culture dependent methods and molecular techniques. A total of 270 isolates were confirmed as Vibrio genus delineating into V. cholerae (27%), V. parahaemolyticus (9.1%), V. vulnificus (4.1%), and V. fluvialis (3%). The remainder (>50%) may account for other Vibrio species not identified in the study. The four Vibrio species were isolated from secondary hospital wastewater effluent (SHWE), while V. cholerae was the sole specie isolated from Limbede community wastewater effluent (LCWE) and none of the four Vibrio species was recovered from tertiary hospital wastewater effluent (THWE). However, several virulence genes were identified among V. cholerae isolates from SHWE: ToxR (88%), hylA (81%), tcpA (64%), VPI (58%), ctx (44%), and ompU (34%). Virulence genes factors among V. cholerae isolates from LCWE were: ToxR (78%), ctx (67%), tcpA (44%), and hylA (44%). Two different genes (vfh and hupO) were identified in all confirmed V. fluvialis isolates. Among V. vulnificus, vcgA (50%) and vcgB (67%) were detected. In V. parahaemolyticus, tdh (56%) and tlh (100%) were also identified. This finding reveals that the studied aquatic niches pose serious potential health risk with Vibrio species harbouring virulence signatures. The distribution of virulence genes is valuable for ecological site quality, as well as epidemiological marker in the control and management of diseases caused by Vibrio species. Regular monitoring of HWW and communal wastewater effluent would allow relevant establishments to forecast, detect, and mitigate any public health threats in advance.
Validating Interactions of Pathogenic Proteins of Staphylococcus aureus and E. coli with Phytochemicals of Ziziphus jujube and Acacia nilotica
Authors: Zou Wen , Hassan Iram , Akram Bushra , Sattar Huma , Altaf Awais , Aqib Amjad Islam , Aslam Hassaan Bin , Almutairi Mikhlid H. , Li Kun
Published: September 2023

This study focused on the assessment of the antimicrobial resistance of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) isolated from bovine mastitis milk samples and the revealing anti-mastitis potential of phytocompounds of Ziziphus jujube and Acacia nilotica through molecular docking analysis. The mastitis milk samples were collected from various dairy farms for the isolation of the bacteria (S. aureus and E. coli) and their response to antibiotics. Ethanolic extracts of both plants were prepared. Their antibacterial activity was evaluated, and they were processed for phytochemical analysis after which, molecular docking analysis with pathogenic proteins of the bacteria was carried out. Parametric and non-parametric statistical analyses were performed to reach the conclusions of this study. The findings of the study revealed a higher drug resistance (≥40%) of E. coli against ampicillin, amikacin, and vancomycin, while S. aureus exhibited the highest resistance to ampicillin, erythromycin, and ciprofloxacin. The ethanolic extracts of the Ziziphus jujube and Acacia nilotica plants produced a ZOI between 18 and 23 mm against multidrug-resistant S. aureus and E. coli. Gas chromatography–mass spectrophotometry (GC–MS) was used to explore 15 phytocompounds from Ziziphus jujube and 18 phytocompounds from Acacia nilotica. The molecular docking analysis of 2cyclopenten−1-one,3,4,4 trimethyl and Bis (2ethylhexyl) phthalate of Ziziphus jujube showed a binding affinity of −4.8 kcal/mol and −5.3 kcal/mol and −5.9 kcal/mol and −7.1 kcal/mol against the DNA Gyrase and toxic shock syndrome toxin-1 proteins of S. aureus and E. coli, respectively. The suberic acid monomethyl ester of Acacia nilotica showed a binding affinity of −5.9 kcal/mol and −5 kcal/mol against the outer membrane protein A and Topoisomerase IV protein of E. coli and −5.1 kcal/mol and −5.8 kcal/mol against the toxic shock syndrome toxin-1 and Enterotoxin B proteins of S. aureus. Similarly, 2,2,4-trimethyl-1,3-pentanediol di-iso-butyrate showed a binding affinity of −6.5 kcal/mol and −5.3 kcal/mol against the outer membrane protein A and Topoisomerase IV of E. coli and −5.2 kcal/mol and −5.9 kcal/mol against the toxic shock syndrome toxin-1 and Enterotoxin B proteins of S. aureus, respectively. The study concluded that there was an increasing trend for the antimicrobial resistance of S. aureus and E. coli, while the Ziziphus jujube and Acacia nilotica plant extracts expressed significant affinity to tackle this resistance; hence, this calls for the development of novel evidence-based therapeutics.
Effects of Male and Female Strains of Salix linearistipularis on Physicochemical Properties and Microbial Community Structure in Saline–Alkali Soil
Authors: Cui Haojun , Li Yan , Wang Wenyi , Chen Lili , Han Zhouqing , Ma Shurong , Wang Weidong
Published: September 2023

The woody plant gender difference may lead to alteration in rhizosphere microbial communities and soil physicochemical properties. In this study, we investigated the differences in rhizosphere soil properties and microbial community structures of S. linearistipularis. Rhizosphere microorganisms were analyzed by high-throughput sequencing technology. The results showed that there were significant differences in rhizosphere soil nutrition between male and female S. linearistipularis plants in saline–alkali soil. The female S. linearistipularis plants significantly reduce soil pH values and significantly increase the soil water content (SWC), available total nitrogen (TN), soil organic matter (SOM), and soil urease activity (S-UE) compared to the male plant. The ACE, Chao, and Shannon index of the female plant was significantly higher than that of the male strain. At the level of Bacteriophyta, the relative abundance of Actinobacteriota in male and female S. linearistipularis was the highest, with 34.26% and 31.03%, respectively. Among the named bacterial genera, the relative abundance of Defluviicoccus of male and female plants was the highest, with 2.67% and 5.27%, respectively. At the level of Eumycophyta, the relative abundance of Ascomycetes in male and female plants was the highest, with 54.93% and 52.10%, respectively. Among the named fungi genera, the relative abundance of male and female plants of Mortierella was the highest, with 6.18% and 9.31%, respectively. In addition, soil pH, SOM, SWC, and S-UE activities were the main driving factors of soil microbial community structures. In the process of restoring saline–alkali land in the Songnen Plain, we may prioritise the planting of female S. linearistipularis, which also provides a theoretical basis for the microorganisms restoration of saline–alkali land in the Songnen plain.
Gold Leaching from Printed Circuit Boards Using a Novel Synergistic Effect of Glycine and Thiosulfate
Authors: Godigamuwa Kasun , Okibe Naoko
Published: September 2023

Printed circuit boards (PCBs) are a secondary source for the extraction of precious metals, such as gold (Au), silver (Ag), platinum (Pt), palladium (Pd), etc. Thiosulfate and glycine systems have recently gained a significant amount of attention for Au leaching. However, in the thiosulfate system, the stability of leached Au tends to decrease when using lower thiosulfate concentrations. In this study, a copper–ammonia–thiosulfate system (thiosulfate system) and glycine or histidine were combined to leach Au from PCBs. The glycine–thiosulfate system resulted in a higher Au leaching than the histidine–thiosulfate system. The results revealed that the glycine–thiosulfate system exhibited a synergistic effect on Au leaching (93.7%) at pH 9.3 and 40 °C, while the Au leaching percentages were 47.1% and 50.7% for the thiosulfate and glycine systems, respectively. In the dual system, Fe leaching was insignificant, although Ag and Al leaching were 95.3% and 27.0%, respectively. Compared to the thiosulfate system, the dual system maintained the stability of the leached Au. The system required 60 mM thiosulfate and 0.5 M glycine at 40 °C and pH 9.3 in order to leach Au from PCBs. The kinetic study suggested that Au and Ag leaching from PCBs in the dual system followed the diffusion-controlled model. The Au leaching rate in the initial phase of the dual system was similar to that of the glycine–cyanide system. This novel, mild approach could be applied to hydrometallurgy to leach other precious metals from sources, such as ore and spent catalysts.
Editorial for the Special Issue “Isotopic Tracers of Mantle and Magma Evolution”
Authors: Li Zhuang
Published: September 2023

Over the past few decades, an increasing number of isotopic studies (Re–Os, Lu–Hf, Sm–Nd, etc [...]
Integration of Whole-Rock Geochemistry and Mineral Chemistry Data for the Petrogenesis of A-Type Ring Complex from Gebel El Bakriyah Area, Egypt
Authors: Abd El-Fatah Ahmed A. , Surour Adel A. , Azer Mokhles K. , Madani Ahmed A.
Published: September 2023

El Bakriyah Ring Complex (BRC) is a prominent Neoproterozoic post-collisional granite suite in the southern part of the Central Eastern Desert of Egypt. The BRC bears critical materials (F, B, Nb, and Ta) in appreciable amounts either in the form of rare-metals dissemination or in the form of fluorite and barite vein mineralization. The complex consists of inner syenogranite and outer alkali feldspar granite that have been emplaced in a Pan-African assemblage made up of granitic country rocks (granodiorite and monzogranite), in addition to post-collisional fresh gabbro as a part of the Arabian-Nubian Shield (ANS) in northeast Africa. Granites of the BRC are characterized by enrichment in silica, alkalis, Rb, Y, Ga, Nb, Ta, Th, and U and depletion in Sr, Ba, and Ti. Geochemical characterization of the BRC indicates that the magma is a crustal melt, which originated from the partial melting of metasedimentary sources. Concentrations of rare-earth elements (REEs) differ in magnitude from the ring complex and its granitic country rocks but they have similar patterns, which are sub-parallel and show LREEs enrichment compared to HREEs. The presence of a negative Eu anomaly in these rocks is related to plagioclase fractionation. The abundance of fluorine (F) in the different granite varieties plays an important role in the existence of a tetrad influence on the behavior of REEs (TE1, 3 = up to 1.15). Geochemical parameters suggest the crystallization of the BRC granite varieties by fractional crystallization and limited assimilation. Mn-columbite and Mn-tantalite are the most abundant rare-metals dissemination in the BRC granite varieties. We present combined field, mineralogical and geochemical data that are in favor of magma originating from a metasedimentary source for the BRC with typical characteristics of A-type granites. Our geodynamic model suggests that the Gebel El Bakriyah area witnessed the Neoproterozoic post-collisional stage of the ANS during its late phase of formation. This stage was characterized by the emplacement of fresh gabbros followed by the syenogranite and alkali-feldspar granite of the BRC into an arc-related assemblage (granodiorite and monzogranite). It is believed that the mantle-derived magma was interplated and then moved upward in the extensional environment to a shallower level in the crust owing to events of lithospheric delamination. This presumably accelerated the processes of partial melting and differentiation of the metasedimentary dominated source (Tonian-Cryogenian) to produce the A-type granites building up the BRC (Ediacaran).
NdFeB Permanent Magnet Uses, Projected Growth Rates and Nd Plus Dy Demands across End-Use Sectors through 2050: A Review
Authors: Heim James W. , Vander Wal Randy L.
Published: September 2023

Rare earth element (REE) permanent magnets (NdFeB) are a critical element in a vast and growing number of industrial applications. In consumer electronics, a broad category encompassing computer, CD, and DVD hard drives, in addition to the ubiquitous cell phones, the nominal NdFeB magnet content may be small, but the global market share for this sector accounts for almost 30% of NdFeB demand, due to a large and continually increasing consumer base. It is estimated that wind turbines that primarily employ permanent magnets will add roughly 110 GW annually of on- and off-shore capability over the next few years. Electric vehicles (EVs) and E-bicycles (EBs) equipped with permanent magnet motors comprise the transportation contribution. Permanent magnet motors have garnered nearly 100% of the market share among EV manufacturers worldwide. Industrial, professional service, and personal robots, most using permanent magnets, are also included in the projected global need for rare earths, particularly Nd and Dy. The sector projects significant growth of approximately 10% across robotic categories. In this paper, we calculate the future demand for Nd and Dy through 2050 across these sectors using a compounded annual growth rate coupled with magnet weight and rare earth content. Uncertainties in the estimates, such as the true global production of Nd, a range of end-product scales and/or unit types in each sector, varied magnet compositions, and the variety of uses within a sector, are all considered.
Flotation Behavior and Interface Characteristics of Apatite with Co-Depression by Sulfuric Acid and Phosphoric Acid
Authors: Lai Xiangping , Cheng Wei , Pan Xueling , Huang Wenhao
Published: September 2023

Phosphate ore is an important strategic mineral resource. The efficient utilization of phosphate resources faces challenges such as low grade of raw ore and difficulty in discharging gangue minerals. One of the key problems to be solved urgently in the reverse flotation of phosphate ore is the effective depression of apatite. However, research on the influence mechanism of acid depressants on the surface properties and adsorption characteristics of apatite is still insufficient. In this study, the influence of different depressants (such as sulfuric acid, phosphoric acid or mixed acid of sulfur acid and phosphorus acid) on the flotation separation performance of an artificial mixture of apatite and dolomite (gangue mineral) was investigated through laboratory flotation tests. On this basis, with the addition of different depressants, the contact angle, zeta potential, XPS and TOC were used to investigate the surface wettability, surface charge, surface species and the adsorption characteristics of the collector (sodium oleate) on the apatite surface, and, accordingly, the inhibiting mechanism was discussed. The results show that, when mixed acid of sulfur acid and phosphorus acid is used as a depressant, a concentrate with a P2O5 grade of 33.53% and a recovery of 88.92% can be obtained, and the parameters are better than when using phosphoric acid with a P2O5 grade of 30.15% and a recovery of 80.12% or sulfuric acid with a P2O5 grade of 30.12% and a recovery of 80.58%. Our analysis shows that the mixed acid has the best inhibiting effect on apatite, which is mainly due to the following: (a) after adding the mixed acid, chemicals such as CaSO4, CaHPO4/Ca(H2PO4)2 are generated on the surface of apatite, resulting in a significant reduction in the contact angle and stronger surface hydrophilicity; (b) the mixed acid reduces the zeta potential of apatite, produces new species and weakens the non-selective adsorption of negatively charged oleate on the surface of apatite, thus preventing the apatite from floating.