bluuebunny/crossref_metadata_2025 huggingface dataset:
The digitization process and the evolution of Clinical Risk Management concept: The role of Clinical Engineering in the operational management of biomedical technologies
Authors: Verga Matteo , Viganò Gian Luca , Capuzzo Martina , Duri Claudia , Ignoti Lucia Maria , Picozzi Paola , Cimolin Veronica
Published: February 2023

Introduction

Digital transformation and technological innovation which have influenced several areas of social and productive life in recent years, are now also a tangible and concrete reality in the vast and strategic sector of public healthcare. The progressive introduction of digital technologies and their widespread diffusion in many segments of the population undoubtedly represent a driving force both for the evolution of care delivery methods and for the introduction of new organizational and management methods within clinical structures.

Methods

The CS Clinical Engineering of the “Spedali Civili Hospital in Brescia” decided to design a path that would lead to the development of a software for the management of biomedical technologies within its competence inside the hospital. The ultimate aim of this path stems from the need of Clinical Engineering Department to have up-to-date, realistic, and systematic control of all biomedical technologies present in the company. “Spedali Civili Hospital in Brescia” is not just one of the most important corporate realities in the city, but it is also the largest hospital in Lombardy and one of the largest in Italy. System development has followed the well-established phases: requirement analysis phase, development phase, release phase and evaluating and updating phase.

Results

Finally, cooperation between the various figures involved in the multidisciplinary working group led to the development of an innovative management software called “SIC Brescia”.

Discussion

The contribution of the present paper is to illustrate the development of a complex implementation model for the digitization of processes, information relating to biomedical technologies and their management throughout the entire life cycle. The purpose of sharing this path is to highlight the methodologies followed for its realization, the results obtained and possible future developments. This may enable other realities in the healthcare context to undertake the same type of pathway inspired by an accomplished model. Furthermore, future implementation and data collection related to the proposed Key Performance Indicators, as well as the consequent development of new operational management models for biomedical technologies and maintenance processes will be possible. In this way, the Clinical Risk Management concept will also be able to evolve into a more controlled, safe, and efficient system for the patient and the user.
Sarcopenic obesity is associated with coffee intake in elderly Koreans
Authors: Lee Do-Youn , Shin Sunghoon
Published: February 2023

This study assessed the association between sarcopenic obesity (S+O+) and coffee intake inelderly Koreans. This study obtained data from the Korea National Health and Nutrition Examination Survey (KNHANES, 2008–2011), a cross-sectional and nationally representative survey conducted by the Korean Centers for Disease Control and Prevention. Of the 2,661 participants included in this study, there was a significant difference between 5.861 (95% CI 2.024–16.971) in less than one cup of coffee, and 6.245 (95% CI 2.136–18.260) in one cup of coffee, and 4.323 (95% CI 1.457–12.824) in two cups of coffee compared to three or more than cups of coffee. In contrast, in the case of sarcopenia or obesity only (S+O- or S-O+), no significant difference was found in any model. The results suggest that the elderly who consume less than one cup of coffee per day had a greater risk of S+O+ than those who consume more than three cups per day. Furthermore, there was an association between coffee intake and sarcopenia but not with obesity. Therefore, coffee intake may have prevented musculoskeletal loss in these patients.
Patient experiences receiving rehabilitation care via telehealth: Identifying opportunities for remote care
Authors: Goldman Jennifer G. , Merkitch Douglas , Brewington David , Peirce Hannah , Rho Monica , Jayabalan Prakash , Curran Jessica , Brennan Kimberly
Published: February 2023

Use of telehealth has grown substantially in recent times due to the COVID-19 pandemic. Remote care services may greatly benefit patients with disabilities; chronic conditions; and neurological, musculoskeletal, and pain disorders, thereby allowing continuity of rehabilitation care, reducing barriers such as transportation, and minimizing COVID-19 exposure. In March 2020, our rehabilitation hospital, Shirley Ryan AbilityLab, launched a HIPAA-compliant telemedicine program for outpatient and day rehabilitation clinics and telerehabilitation therapy programs. The objective of this study was to examine patients' experiences and satisfaction with telemedicine in the rehabilitation physician practice, including novel virtual multidisciplinary evaluations. The present study examines survey data collected from 157 patients receiving telemedicine services at Shirley Ryan AbilityLab from December 2020–August 2021. Respondents were 61.8% female, predominantly White (82.2%) with ages ranging across the lifespan (69.4% over age 50 years). Diagnostic categories of the respondents included: musculoskeletal conditions 28%, chronic pain 22.3%, localized pain 10.2%, neurological conditions 26.8%, and Parkinson's and movement disorders 12.7%. Survey responses indicate that the telemedicine experiences were positive and well received. The majority of participants found these services easy to use, effective, and safe, and were overall satisfied with the attention and care they received from the providers—even for those who had not previously used telehealth. Respondents identified a variety of benefits, including alleviating financial and travel-related burdens. There were no significant differences in telehealth experiences or satisfaction across the different clinical diagnostic groups. Respondents viewed the integrated physician and rehabilitation therapist telehealth multidisciplinary model favorably, citing positive feedback regarding receiving multiple perspectives and recommendations, feeling like an integrated member of their healthcare team, and having a comprehensive, holistic team approach along with effective communication. These findings support that telemedicine can provide an effective care model in physiatry (physical medicine and rehabilitation) clinics, across different neurological, musculoskeletal, and pain conditions and in multidisciplinary team care settings. The insights provided by the present study expand our understanding of patient experiences with remote care frameworks for rehabilitation care, while controlling for institutional variation, and ultimately will help provide guidance regarding longer term integration of telemedicine in physiatry and multidisciplinary care models.
Prelinguistic intersubjective and socio-communicative skills in infants with neurodevelopmental disabilities aged 0–36 months: A new assessment and parent support tool
Authors: Strazzer Sandra , Sacchi Daniela , Rigamonti Roberta , Miccoli Annalisa , Bonino Margherita , Giancola Serena , Germiniasi Chiara , Montirosso Rosario
Published: February 2023

Background

Although children with neurodevelopmental disability (NDD) present with several deficits, they partially share developmental impairments in prelinguistic intersubjective and socio-communicative skills, which are not easily assessed by conventional tests during the first years of life.

Aim

The current paper presents a new procedure to assess the prelinguistic intersubjective and socio-communicative skills of NDD children aged 0–36 months. A specific observation form template, called the Observation of Prelinguistic Intersubjective and Socio-Communicative Skills (OPISCoS) form, has been designed to systematically detect infant skills during daily routines (e.g., mealtime, playtime, desk activities). The OPISCoS form helps speech therapists to provide parents support to better perceive and understand early communicative signals from their children, avoiding the risk of excessive or reduced social stimulation.

Methods

The OPISCoS form is composed of three sections, namely, “Pragmatics and Communication,” “Decoding,” and “Expression,” which are useful to delineate the communication abilities of children with NDD and are not tapped by traditional batteries. Vignettes from clinical practice illustrate and provide exemplifications for using the OPISCoS form with NDD infants and their parents.

Results

The OPISCoS form was reported for two children and showed potential in detecting disrupted communicative behaviors and planning specific early interventions. Further, we observed an improvement not only in children's communicative abilities improve but also in their interactions with parents. From a clinical point of view, the OPISCoS form (1) offers an observational perspective of prelinguistic intersubjective and socio-communicative skills in infants with NDD and (2) may be useful to practitioners to enhance parents’ sensitivity to their infants’ communicative behavior.

Conclusion

The OPISCoS form was developed in clinical practice and is based on a very preliminary description of a new observational procedure as integration for the assessment of NDD children. The OPISCoS form appears to be a useful tool for the clinical assessment of prelinguistic intersubjective and socio-communicative skills in NDD infants as well as for promoting the quality of early parenting.
Blood donation during COVID-19 lockdown and its association with anger and stress: A cross-sectional study from Syria
Authors: Kakaje Ameer , Mansour Sabina , Ghareeb Ayham , Hosam Aldeen Osama
Published: February 2023

Background

The demand for blood donations in Syria was high as the country has suffered for 9 years from war, and this demand has substantially increased during and after the lockdown from the COVID-19 pandemic. This study aims to assess blood donations in Syria and their association with multiple factors.

Methods

Online questionnaires were distributed to social media groups, with questions related to stress, anger, and COVID-19 distress.

Results

This study included 1,423 participants, of which 899 (63.2%) were women. Only 48.5% have ever donated blood, of which 33.3% donated only once in their lifetime. Not having a good reason to donate blood was the main reason for not donating. Obtaining documents was the main reason for blood donation among people who donated blood (64.8%). Stress, anger, and fear of infection were not associated with blood donation and its patterns. Losing someone close and being endangered by war were associated with more frequent blood donations in contrast to being distressed by war.

Conclusion

COVID-19 distress, stress, and anger were not associated with the decrease in blood donation. Spreading awareness on volunteer blood donation is crucial to combat blood shortage during stressful times.
Isolation of Leptospira kirschneri serovar Grippotyphosa from a red panda (Ailurus fulgens) after antimicrobial therapy: Case report
Authors: LeCount Karen , Fox Kami , Anderson Tammy , Bayles Darrell O. , Stuber Tod , Hicks Jessica , Schlater Linda K. , Nally Jarlath E.
Published: February 2023

A 1-year-old female red panda started showing symptoms of illness, including lethargy, anorexia, abdominal discomfort, and vomiting, shortly after transfer to a new zoo. Serum was tested for leptospirosis using the microscopic agglutination test, and a titer of 1:25,600 to serogroup Grippotyphosa was detected. Antimicrobial treatment with doxycycline was initiated. After completion of treatment and resolution of clinical symptoms, a urine sample was collected to ensure clearance of leptospires and cessation of urinary shedding prior to co-housing with other red pandas. A repeat serum sample taken 13 days later had a lower titer of 1:6,400 to serogroup Grippotyphosa. A sample of the animal's urine was cultured in HAN media and was culture positive for Leptospira. The recovered isolate was completely characterized by whole genome sequencing and serotyping with reference antisera, and the isolate was classified as Leptospira kirschneri serogroup Grippotyphosa serovar Grippotyphosa strain RedPanda1.
Clinical response in dogs with acute hemorrhagic diarrhea syndrome following randomized probiotic treatment or fecal microbiota transplant
Authors: Jugan Maria C. , KuKanich Kate , Freilich Leah
Published: February 2023

Probiotics and fecal microbiota transplants (FMTs) are two microbiome-targeted therapies that have been investigated for use in gastrointestinal diseases associated with dysbiosis. The aim of this study was to compare the effects of an oral multi-strain probiotic and enema-administered FMTs on clinical signs and serum lipopolysaccharide in dogs with acute hemorrhagic diarrhea syndrome (AHDS). A total of 18 client-owned dogs with a diagnosis of AHDS were enrolled in a randomized, blinded study at the time of hospital admission. The dogs were randomized into two groups: the probiotic group received a daily oral probiotic (200 × 109 CFU/10kg q 24 h) for 14 days and a single sham enema; the FMT group received a single FMT via retention enema (10 mL/kg) and placebo oral capsule for 14 days. All dogs received concurrent standard-of-care therapy, including intravenous fluids and anti-emetics; no dogs received antimicrobials. The fecal score, disease severity scores, and serum lipopolysaccharide were measured on days 0, 3, and 14. Fourteen of eighteen enrolled dogs completed the study (n = 9 probiotics; n = 5 FMT). Lipopolysaccharide decreased on days 3 and 14 from baseline and correlated with fecal and disease severity scores. There was no difference in the duration or severity of clinical signs in dogs with AHDS following an enema-administered FMT compared to probiotic treatment. Further evaluation of serum lipopolysaccharide as a marker of disease severity and recovery is warranted.
Utility of diagnostic tests in vomiting dogs presented to an internal medicine emergency service
Authors: Holzmann Bettina , Werner Melanie , Unterer Stefan , Dörfelt René
Published: February 2023

Introduction

Vomiting is a common sign in dogs presenting to emergency services. It can be self-limiting, a sign of a life-threatening extraintestinal, or intestinal disorder. Reasonable diagnostics should be performed to determine the underlying cause. This study aimed to assess the utility of diagnostic tests in vomiting dogs, and its correlation with patient history, and physical examination results. Additionally, parameters to differentiate uncomplicated vomiting from complicated vomiting were investigated.

Methods

In this prospective, observational, clinical study, data from 99 client-owned dogs with vomiting, presenting as first opinion cases, were evaluated. History, physical examination, duration of clinical signs, overall number of episodes of vomiting, appetite, and additional clinical signs were recorded. The standardized diagnostic evaluation of all patients included venous blood gas analysis, complete blood count, serum biochemistry profile, canine pancreatic lipase, abdominal radiographs, ultrasound, and urinalysis. Follow-up was performed 4–5 days later. Based on severity of disease and clinical course, dogs were categorized to “uncomplicated vomiting” (UN), or “complicated vomiting” (COM). The utility of each test for diagnosing the cause of vomiting was evaluated. Spearman correlation coefficient, Chi-squared-, unpaired t-, and Mann–Whitney U-test were used. Statistical significance was defined as p ≤ 0.05.

Results

Out of the 99 dogs, 34 had uncomplicated courses of disease (UN). In 60/99 cases, a diagnosis was obtained, and in 39/99 cases, the cause for vomiting remained unknown. Longer duration of clinical signs, and reduced appetite were associated with higher utility of abdominal ultrasound. A poor mentation was associated with a higher utility of blood examinations and abdominal radiographs. Dogs presenting with an impaired mentation or with additional clinical signs other than diarrhea, were more likely to be in the COM group.

Discussion

Based on this investigation, general recommendations concerning the diagnostic approach for patients with vomiting could not be provided. For dogs who have exclusively vomiting as a clinical sign, and present in good mentation, further investigations might not be beneficial, and these dogs may recover with symptomatic treatment alone. Additional diagnostics could be indicated in dogs with additional clinical signs other than diarrhea.
Main factors associated with foot-and-mouth disease virus infection during the 2001 FMD epidemic in Uruguay
Authors: Iriarte María V. , Gonzáles José L. , de Freitas Costa Eduardo , Gil Andrés D. , de Jong Mart C. M.
Published: February 2023

Large epidemics provide the opportunity to understand the epidemiology of diseases under the specific conditions of the affected population. Whilst foot-and-mouth disease (FMD) epidemics have been extensively studied in developed countries, epidemics in developing countries have been sparsely studied. Here we address this limitation by systematically studying the 2001 epidemic in Uruguay where a total of 2,057 farms were affected. The objective of this study was to identify the risk factors (RF) associated with infection and spread of the virus within the country. The epidemic was divided into four periods: (1) the high-risk period (HRP) which was the period between the FMD virus introduction and detection of the index case; (2) the local control measures period (LCM) which encompassed the first control measures implemented before mass vaccination was adopted; (3) the first mass vaccination, and (4) the second mass vaccination round. A stochastic model was developed to estimate the time of initial infection for each of the affected farms. Our analyses indicated that during the HRP around 242 farms were probably already infected. In this period, a higher probability of infection was associated with: (1) animal movements [OR: 1.57 (95% CI: 1.19–2.06)]; (2) farms that combined livestock with crop production [OR: 1.93 (95% CI: 1.43–2.60)]; (3) large and medium farms compared to small farms (this difference was dependent on regional herd density); (4) the geographical location. Keeping cattle only (vs farms that kept also sheep) was a significant RF during the subsequent epidemic period (LCM), and remained as RF, together with large farms, for the entire epidemic. We further explored the RF associated with FMDV infection in farms that raised cattle by fitting another model to a data subset. We found that dairy farms had a higher probability of FMDV infection than beef farms during the HRP [OR: 1.81 (95% CI: 1.12–2.83)], and remained as RF until the end of the first round of vaccination. The delay in the detection of the index case associated with unrestricted animal movements during the HRP may have contributed to this large epidemic. This study contributes to the knowledge of FMD epidemiology in extensive production systems.
Effect of indoor house dust mite concentration on canine atopic dermatitis
Authors: Kim Jihee , Lee Ji-Hye , Song Yunji , Kim Ha-Jung
Published: February 2023

Introduction

House dust mites (HDM) are regarded as essential environmental allergens not only in human, but also in canine atopic dermatitis (CAD), however, there are only a few studies on the influence of indoor HDM concentration on the disease.

Methods

Our study analyzed the correlation between the indoor HDM concentration, the severity of CAD, and the residential environments in client-owned 35 AD and 13 healthy dogs. We measured the extent of CAD and severity index-04 (CADESI-04), pruritus visual analog scale (PVAS), and transepidermal water loss (TEWL), indoor relative humidity (RH) and analyzed the residential environment questionnaires to evaluate AD severity.

Results

The Der f 1 concentration had an inverse association with TEWL, and no association with CADESI-04 and PVAS. The Der f 1 concentration was significantly high in the group living near the green area and 40% or higher RH.

Discussion

Our results suggest two possibilities: (1) Living around green areas and maintaining an appropriate indoor climate may help to improve CAD clinical symptoms. (2) The HDM may contain endotoxin and when present in high concentrations in CAD, they play a preventive role by enhancing the skin barrier function. Further studies with a larger number of dogs may help further elucidate an association between CAD and Der f 1.
A prognostic 15-gene model based on differentially expressed genes among metabolic subtypes in diffuse large B-cell lymphoma
Authors: Hou Jun , Guo Peng , Lu Yujiao , Jin Xiaokang , Liang Ke , Zhao Na , Xue Shunxu , Zhou Chengmin , Wang Guoqiang , Zhu Xin , Hong Huangming , Chen Yungchang , Lu Huafei , Wang Wenxian , Xu Chunwei , Han Yusheng , Cai Shangli , Liu Yang
Published: February 2023

The outcomes of patients with diffuse large B-cell lymphoma (DLBCL) vary widely, and about 40% of them could not be cured by the standard first-line treatment, R-CHOP, which could be due to the high heterogeneity of DLBCL. Here, we aim to construct a prognostic model based on the genetic signature of metabolic heterogeneity of DLBCL to explore therapeutic strategies for DLBCL patients. Clinical and transcriptomic data of one training and four validation cohorts of DLBCL were obtained from the GEO database. Metabolic subtypes were identified by PAM clustering of 1,916 metabolic genes in the 7 major metabolic pathways in the training cohort. DEGs among the metabolic clusters were then analyzed. In total, 108 prognosis-related DEGs were identified. Through univariable Cox and LASSO regression analyses, 15 DEGs were used to construct a risk score model. The overall survival (OS) and progression-free survival (PFS) of patients with high risk were significantly worse than those with low risk (OS: HR 2.86, 95%CI 2.04–4.01, p < 0.001; PFS: HR 2.42, 95% CI 1.77–3.31, p < 0.001). This model was also associated with OS in the four independent validation datasets (GSE10846: HR 1.65, p = 0.002; GSE53786: HR 2.05, p = 0.02; GSE87371: HR 1.85, p = 0.027; GSE23051: HR 6.16, p = 0.007) and PFS in the two validation datasets (GSE87371: HR 1.67, p = 0.033; GSE23051: HR 2.74, p = 0.049). Multivariable Cox analysis showed that in all datasets, the risk model could predict OS independent of clinical prognosis factors (p < 0.05). Compared with the high-risk group, patients in the low-risk group predictively respond to R-CHOP (p = 0.0042), PI3K inhibitor (p < 0.05), and proteasome inhibitor (p < 0.05). Therefore, in this study, we developed a signature model of 15 DEGs among 3 metabolic subtypes, which could predict survival and drug sensitivity in DLBCL patients.
Parallel Algorithm for Connected-Component Analysis Using CUDA
Authors: Windisch Dominic , Kaever Christian , Juckeland Guido , Bieberle André
Published: February 2023

In this article, we introduce a parallel algorithm for connected-component analysis (CCA) on GPUs which drastically reduces the volume of data to transfer from GPU to the host. CCA algorithms targeting GPUs typically store the extracted features in arrays large enough to potentially hold the maximum possible number of objects for the given image size. Transferring these large arrays to the host requires large portions of the overall execution time. Therefore, we propose an algorithm which uses a CUDA kernel to merge trees of connected component feature structs. During the tree merging, various connected-component properties, such as total area, centroid and bounding box, are extracted and accumulated. The tree structure then enables us to only transfer features of valid objects to the host for further processing or storing. Our benchmarks show that this implementation significantly reduces memory transfer volume for processing results on the host whilst maintaining similar performance to state-of-the-art CCA algorithms.
A Data Analysis Smart System for the Optimal Deployment of Nanosensors in the Context of an eHealth Application
Authors: Lopez Alberto , Aguilar Jose
Published: February 2023

This paper presents the utilization of the Data Analysis Smart System (DASS) of ARMNANO in a nanotechnology application in electronic health. We made a special approach to the liver situation for patients that have been monitored with respect to two variables concerning their liver status: the Mean Corpuscular Volume (MCV) and the Alkaline phosphotas (ALKPHOS). These variables are analyzed using the autonomous cycle “Conditioning Thinking Mode” (CTM), one of the two autonomic cycles of data analysis tasks that make up the DASS. In this sense, an optimization problem is defined to determine the optimal deployment of nanosensors (NSs) for the proper determination of liver status. The application of genetic algorithms (GA) allows us to find the optimal number of NSs in the system to precisely determine the liver status, avoiding a large data volume. In total, we evaluated its implementation in two case studies and carried out a hyperparameterization process for assuring the definition of the key parameters. The greatest propensity is to place NSs in the regions close to the liver, becoming saturated as the amount of SNs increases (they do not improve the quality of the liver status value).
Improved Craig–Bampton Method Implemented into Durability Analysis of Flexible Multibody Systems
Authors: Wang Gengxiang , Niu Zepeng , Feng Ying
Published: February 2023

The Craig–Bampton method is frequently applied in most commercial multibody dynamic software. Nevertheless, the Craig–Bampton modes only represent the free-free modes in flexible multibody systems. However, the free-free modes are incapable of all engineering applications. Hence, a rational set of reference conditions must be correctly chosen to define a unique displacement field. Firstly, a simple 2D beam with two revolute joints is taken as an example to prove that the free-free modes are not suitable for all engineering applications, and the results are validated by ANSYS and the analytical solution. Secondly, the Craig–Bampton method is improved by two different methods: (i) the reference conditions are added to the original Craig–Bampton matrix and (ii) the reference conditions are applied to the shape functions to redefine the mass and stiffness matrices before constructing the original Craig–Bampton matrix. This implementation illustrates that the improved Craig–Bampton matrix can not only generate the free-free modes but is also suitable for the non-free-free modes. Finally, two discrepant reference conditions are imposed to obtain the dynamic response of the flexible connecting rod based on the improved Craig–Bampton method, which is validated using the normal mode approach. Simulations show that the improved Craig–Bampton method can be used as a general-purpose method in durability analysis.
Determining Spatial Relationships between Airports and Local Economy from Competitiveness Perspective: A Case Study of Airports in China
Authors: Wu Zhen , Lai Po-Lin , Ma Fei , Park Keun-Sik , Nimsai Suthep
Published: February 2023

The intent of this research is to present an investigation into whether spatial relationships in China’s airports are primarily characterised by competition or complementarity; accordingly, this is approached from the perspectives of passenger and cargo traffic. This research also focused on two issues: The first is the spatial Durbin model (SDM), which is used to judge the competition or complementarity among airports with spatial dependence as an indicator. Second, considering that airport activities may be affected by neighbouring cities due to the externality of economic development, the spillover effects of different geo-economic factors at the city level are calculated. Through the utilisation of a spatial Durbin model and yearly airport traffic data for 34 Chinese airports between 2007 and 2019, it was found that the nature of spatial relationships tends to differ regarding passengers and cargo traffic. Concerning passenger traffic, airports in China are mainly characterised by complementarity and are competitive regarding cargo traffic. This study also indicates that the geo-economic factors of central cities (Beijing, Shanghai, and Guangzhou) can affect the spatial relationship between China’s airports. China’s airports are more dependent on the economic development of central cities, and therefore more dependent on the traffic replenishment at China’s hub airports. In addition, the validity of the asymmetrical economic weight matrix illustrates that after controlling the exogenous interaction effects between the independent variables and dependent variables, the difference of regional economic development and airport traffic does lead to endogenous interaction effects among China’s airports.
Development of a General Prediction Model of Moisture Content in Maize Seeds Based on LW-NIR Hyperspectral Imaging
Authors: Wang Zheli , Li Jiangbo , Zhang Chi , Fan Shuxiang
Published: February 2023

Moisture content (MC) is one of the important indexes to evaluate maize seed quality. Its accurate prediction is very challenging. In this study, the long-wave near-infrared hyperspectral imaging (LW-NIR-HSI) system was used, and the embryo side (S1) and endosperm side (S2) spectra of each maize seed were extracted, as well as the average spectrum (S3) of both being calculated. The partial least square regression (PLSR) and least-squares support vector machine (LS-SVM) models were established. The uninformative variable elimination (UVE) and successive projections algorithm (SPA) were employed to reduce the complexity of the models. The results indicated that the S3-UVE-SPA-PLSR and S3-UVE-SPA-LS-SVM models achieved the best prediction accuracy with an RMSEP of 1.22% and 1.20%, respectively. Furthermore, the combination (S1+S2) of S1 and S2 was also used to establish the prediction models to obtain a general model. The results indicated that the S1+S2-UVE-SPA-LS-SVM model was more valuable with Rpre of 0.91 and RMSEP of 1.32% for MC prediction. This model can decrease the influence of different input spectra (i.e., S1 or S2) on prediction performance. The overall study indicated that LW-HSI technology combined with the general model could realize the non-destructive and stable prediction of MC in maize seeds.
Adoption of Partial Shook Swarm in the Integrated Control of American and European Foulbrood of Honey Bee (Apis mellifera L.)
Authors: Mosca Michela , Bubnic Jernej , Giannetti Luigi , Fortugno Luca , Pietropaoli Marco , Manara Veronica , Bonerba Elisabetta , Formato Giovanni
Published: February 2023

American foulbrood (AFB) and European foulbrood (EFB) are the two most important bacterial diseases that affect honey bee brood. The aim of this study was to evaluate the effects of an integrated treatment of AFB and EFB in naturally infected colonies in spring, using the beekeeping technique partial shook swarm combined with oxytetracycline treatment. The following parameters were assessed: strength of the colonies, consumption of antibiotic solution, queen losses, honey production and relapses. An increase in strength was observed in both (AFB and EFB) groups. Mean syrup consumption in the AFB colonies was 444.2 mL, while in the EFB colonies it was 497.3 mL. Linear correlation between the strength of the colonies and consumption (p-values < 0.0001; R² = 0.448) was observed. Three queens out of thirty (10%) were lost during the shook swarm procedure: one in the AFB group (5.3%) and two in the EFB group (18.2%). The results highlighted four clinical relapses (22.2%) of AFB, and no relapse (0%) of EFB after two years of observation. Six colonies (four in the AFB group and two in the EFB group) produced honey in the same beekeeping season that the shook swarm and antibiotic treatment were performed. Partial shook swarm is a good alternative to colony destruction, especially for EFB, and provides productive colonies during the foraging season.
Evaluation of Ultrasonic Sensor for Precision Liquid Volume Measurement in Narrow Tubes and Pipes
Authors: Smith Benjamin C. , Bergman Ryan W. , Darr Matthew J.
Published: February 2023

The introduction of computer vision and machine learning into agricultural systems has produced significant new opportunities for high precision application of liquid products in both grain and livestock agriculture. These technologies, which enable liquid application in site-specific, non-broadcast applications, are driving new evaluations of nozzle technologies which apply a consistent dose of liquid product in a non-conventional manner compared to historic perceptions. This field of innovation is driving the need for improved high-capacity systems for evaluating nozzle performance in high-precision applications. Historically, patternator tables with volumetric measurements of total applied liquid have served as the standard for fluid nozzle evaluation. These volumetric measurements are based on measuring the displaced distance of liquid over a defined time to determine flow rate. However, current distance sensors present challenges for achieving small-volume measurements and enabling automation at a scale necessary to meet innovation demands of high-precision nozzle systems. A novel concept for high speed and automated measurement of a high precision patternator table was developed using an ultrasonic sensor and a carefully designed liquid retainment system to maximize measurement precision. The performance of this system was quantified by comparing calibrations and performance across different vessels for volume measurement (tubes and pipes) used in the application of a nozzle patternator. A total of three square tubes (15.9, 22.3, 31.0 mm widths) and three pipes (25.2, 27.0, 35.1 mm diameters) were evaluated, with the 27 mm pipe matching the ultrasonic sensor’s rating. All calibrations were successful, depicting linear characteristics with R2 > 0.99. The smallest pipe presented issues for the sensor to measure in post-calibration and was thus not evaluated further. The residual values from operational performance highlight that the 25.2 mm tube and the 27.0 mm pipe are highly accurate with no indication of bias or non-normality. The relative uncertainty ranges from 2.9 to 42% (350 mL to 25 mL) depending on the tube and pipe cross-sectional diameter or width with the sensor accuracy and uncertainty in the tube and pipe area being the largest factors. The results of this study indicate that the 25.2 mm tube and the 27.0 mm pipe could be excellent options for autonomous liquid volume measurement with the ultrasonic sensor. A key challenge identified in this study is that the assumptions in the sensor’s intrinsic calibration are violated with the tubes and pipes evaluated.
Operational, Economic, and Environmental Assessment of an Agricultural Robot in Seeding and Weeding Operations
Authors: Vahdanjoo Mahdi , Gislum René , Sørensen Claus Aage Grøn
Published: February 2023

The development of robotic-based agricultural machinery systems has significantly increased in recent years. Many autonomous systems have not yet been measured based on sustainability and economic performances, even though automation is regarded as an opportunity to increase safety, dependability, productivity, and efficiency. The operational aspect, economic viability, and environmental impact of replacing conventional machinery with robotized alternatives are the primary focus of this study. The robot considered in this research is designed for extensive fieldwork, where PTO and external hydraulics are required. This robot is equipped with two 75 (hp) Kubota diesel engines with a total engine gross power of up to 144 (hp). Both robotic system and conventional machinery were described, and different scenarios were used to examine various operational and environmental indicators, as well as individual cost elements, considering various field sizes and working widths of implements used in seeding and weeding operations. The findings demonstrate that the robotic system outperforms conventional machinery in terms of operational efficiency by as much as 9%. However, the effective field capacity comparison reveals that the conventional system has a field capacity that is up to 3.6 times greater than that of the robotic system. Additionally, the total cost per hour of the robotic system is up to 57% lower than that of the conventional system. The robotic system can save up to 63.3% of fuel during operation, resulting in the same percentage reduction in CO2 emissions as the conventional system, according to a comparison of fuel consumption.
Foliar Application of Carnosine and Chitosan Improving Drought Tolerance in Bermudagrass
Authors: Hao Tian , Yang Zhimin , Liang Jianfeng , Yu Jingjin , Liu Jun
Published: February 2023

Drought stress is one of the crucial factors affecting plant growth and development in turfgrass species, especially during the summer season. Exogenous plant growth regulators are an effective and convenient approach to mitigating the adverse effects of drought stress on plant growth. The objectives of this study were to reveal the effects of exogenous carnosine or chitosan on turf performance and physiological indexes in bermudagrass (Cynodon transvaalensis × C. dactylon) in response to drought stress. Bermudagrass was foliar sprayed with carnosine or chitosan, and dose-dependent effects on turf quality were observed under drought stress. Under drought stress, foliar application of either carnosine (0.03%) or chitosan (10 mg L−1) significantly increased turf quality, chlorophyll content, leaf relative water content, and decreased electrolyte leakage, malonaldehyde, and hydrogen peroxide content in comparison with untreated control in bermudagrass. Moreover, exogenous carnosine treatment significantly enhanced the activities of both catalase and peroxidase, but chitosan application only increased catalase activity. The results of this experiment were beneficial to the development of new plant growth regulators and would provide helpful insights for turf management under drought-stressed conditions.
Detection and Molecular Characterization of Giardia and Cryptosporidium spp. Circulating in Wild Small Mammals from Portugal
Authors: Lux Laura , Ulrich Rainer G. , Santos-Silva Sérgio , Queirós João , Imholt Christian , Klotz Christian , Paupério Joana , Pita Ricardo , Vale-Gonçalves Hélia , Alves Paulo Célio , Mesquita João R.
Published: February 2023

Cryptosporidium spp. and Giardia spp. are important diarrhea-causing protozoan parasites worldwide that exhibit broad host ranges. Wild small mammals can harbor host-adapted and potentially zoonotic species of both parasites. The aim of this study was to investigate Cryptosporidium spp. and Giardia spp. in wild rodents and shrews in Portugal, focusing on the protist’s occurrence and genetic diversity. Molecular screening by PCR at the small subunit (SSU) rRNA gene locus of 290 fecal samples from wood mice (Apodemus sylvaticus), southwestern water voles (Arvicola sapidus), Cabrera’s voles (Microtus cabrerae), Lusitanian pine voles (Microtus lusitanicus), Algerian mice (Mus spretus) and greater white-toothed shrews (Crocidura russula) in Northeast Portugal revealed the low occurrence of Cryptosporidium spp. (1%) and high occurrence of Giardia spp. (32.8%). The analysis revealed that “species” was the only significant factor associated with the increasing probability of Giardia spp. infection, with the highest prevalence reported in southwestern water voles and Lusitanian pine voles. Cryptosporidium and Giardia species determination at the SSU rRNA gene locus revealed C. muris and G. microti as the only circulating species, respectively. Subtyping of the glutamate dehydrogenase (gdh) and beta-giardin (bg) genes provided evidence of the high genetic diversity within the G. microti clade. This study suggests that rodent-adapted G. microti occurs to a large extent in cricetid hosts and supports the limited role of wild rodents and shrews as natural sources of human infections in Northeast Portugal regarding the investigated parasites. Moreover, this is the first record of G. microti in southwestern water voles, Lusitanian pine voles, Algerian mice, wood mice and Cabrera’s voles and C. muris in Cabrera’s voles. Finally, this study improves the database of sequences relevant for the sequence typing of G. microti strains and provides new insights about the epidemiology of Giardia spp. and Cryptosporidium spp. in wild rodents and shrews, two parasite genera of high importance for public and animal health.
Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry
Authors: Zheng Chaojun , Xiao Gengsheng , Yan Xia , Qiu Ting , Liu Shun , Ou Jiancun , Cen Mingzhu , Gong Li , Shi Jiansheng , Zhang Huihua
Published: February 2023

This experiment investigated the benefits of plant essential oil (EO) composed with lauric acid monoglyceride and cinnamaldehyde on necrotic enteritis-challenged broilers. A total of 180 1-day-old healthy yellow-feathered broilers were randomly divided into 4 groups with 3 replicates of 15 chicks each. The experimental groups were as follows: the control group (CON) was fed with the basal diet and was not challenged by Eimeria acervulina (EA) and Clostridium perfringens (CP); CPEA group was also fed with a basal diet, but infected with CP and EA; CPEA_EO350 group and CPEA_EO500 group were fed with a basal diet supplemented with 350 and 500 mg/kg EO, respectively, and all infected with CP and EA. On the 7th day, each bird in the CPEA group, CPEA_EO350 group and CPEA_EO500 group was orally administrated with 1 mL Eimeria acervulina containing 5000 oocytes/mL, and the birds of the CON group were orally administrated with 1 mL normal saline. From the 15th day, 1 mL of CP type A CVCC-2030 strain (about 5 × 108 cfu/mL) was orally inoculated into each bird of the CPEA, CPEA_EO350 and CPEA_EO500 groups for three consecutive days. Similarly, the CON group was orally given 1 mL of normal saline. The CPEA stimulation reduced the average daily gain (ADG) of broilers, increased the feed-to-gain ratio (F:G), and increased the intestinal lesions of the broilers (p < 0.01), indicating that CPEA stimulation was clinically successful. Compared with the CPEA group, the ADG of CPEA_EO350 and CPEA_EO500 increased, the F:G decreased (p < 0.01), and the intestinal score of CPEA_EO500 decreased (p < 0.01). The expression of the tight junction protein of the jejunum and ileum on 21d was upregulated (p < 0.01), and the expression of jejunum inflammation factors TNF-α on 21d and jejunum and ileum inflammatory factor IL-6 on 28d were also downregulated. The CPEA_EO350 and CPEA_EO500 increased antioxidant capacity. To sum up, 350 and 500 mg/kg of lauric acid monoglyceride and cinnamaldehyde complex plant essential oils can improve ADG and F:G, improve intestinal morphology and the body’s antioxidant capacity, and downregulate the expression of inflammatory factors. The concentration of 500 mg/kg performed even better.
Effects of Different Iron Supplements on Reproductive Performance and Antioxidant Capacity of Pregnant Sows as Well as Iron Content and Antioxidant Gene Expression in Newborn Piglets
Authors: Xing Xiaokun , Zhang Chunyong , Ji Peng , Yang Jia , Li Qihua , Pan Hongbin , An Qingcong
Published: February 2023

To improve the reproductive performance of sows and the iron nutrition of newborn piglets, we studied the effects of dietary iron on reproductive performance in pregnant sows as well as antioxidant capacity and the visceral iron content of sows and newborn piglets. Forty pregnant sows were divided into four groups, the iron deficiency group (Id group) was fed a basic diet while sows in the treatment groups were fed diets supplemented with 200 mg/kg lactoferrin (LF group), 0.8% heme-iron (Heme-Fe group), or 500 mg/kg iron-glycine complex (Fe-Gly group). The results indicated that (1) different sources of iron had no significant effect on litter size, live litter size, and litter weight of sows; (2) the three additives improved iron nutrition in newborn piglets, with LF and Heme-Fe having better improvement effects; and (3) the addition of different iron sources improved the level of serum antioxidant biochemical indexes of sows and newborn piglets, and it can have an effect on gene level, among which lactoferrin has the best effect. Thus, adding LF, Heme-iron, or Fe-Gly to the diet of sows during the second and third trimester of gestation can improve the antioxidant capacity of the sows. The supplementation of LF in pregnant sow diets can also improve the antioxidant capacity and the iron nutrition of newborn piglets, with better additive effects than in Heme-Fe and Fe-Gly.
Proximal Ulnar Osteotomy as a Treatment for Humeral Intracondylar Fissure in a Shetland Sheepdog
Authors: Karydas Stavros , Danielski Alan
Published: February 2023

A seven-month-old male Shetland Sheepdog was presented for assessment of thoracic limb lameness of 3 weeks duration. Orthopaedic examination revealed severe discomfort in elbow extension, bilaterally. CT-scan confirmed presence of a complete humeral intracondylar fissure (HIF), bilaterally, and arthroscopic examination of both elbows confirmed the presence of the cartilaginous lesion previously reported in dogs suffering from HIF. A staged oblique proximal ulnar osteotomy was performed to address the humero-anconeal incongruency believed to be the cause of HIF formation. Orthopaedic examination performed 5 weeks after each surgical procedure confirmed that pain previously present on elbow manipulation had subsided. Follow-up examination performed 8 months after the second surgery revealed the dog to be sound at walking on the thoracic limbs with no discomfort present on elbow manipulation. Repeated CT scan confirmed complete healing of both HIFs. This is the first report documenting the presence of HIF in a Shetland sheepdog and complete healing of both HIFs following a proximal ulnar osteotomy.
Vaccination Upregulates Th1 Cytokines in the Lung of Pigs Experimentally Infected with Mycoplasma hyopneumoniae
Authors: Rodríguez Francisco , Rosales Rubén S. , Ramírez Ana S. , Poveda José B.
Published: February 2023

Mycoplasma hyopneumoniae (Mhy) is the causative agent of enzootic pneumonia, characterized by high morbidity and low mortality rates in intensive swine production systems. To better understand the mechanisms underlying the protection of an inactivated whole cell vaccine, we investigated the immunohistochemical differences in the cytokine expression in vaccinated and non-vaccinated pigs experimentally infected with Mhy. Four-week-old Mhy-negative pigs (n = 24) were allocated to negative control (n = 8), or one of two Mhy-infected groups: vaccinated (n = 8) and non-vaccinated (n = 8). Infection was carried out by a combination of trans-tracheal and aerosol route. Lung samples were processed for histopathological and immunohistochemical studies, by using antibodies against Mhy, IL1-α, IL1-β, IL-2, IL-4, IL-5, IL-6, Il-8, IL-10, IL-12p35, IL-13, IL-17A, TNF-α, IFN-γ, and CD-4 lymphocytes. Although all cytokines increased in both infected groups, IL-2, IL-12, and IFN-γ were significantly overexpressed in vaccinated pigs. These findings, in conjunction with the decrease of macroscopic and histological lesions in vaccinated animals, indicate the importance to enhance Th1 response in the immunization strategies to control Mhy infection.