bluuebunny/crossref_metadata_2025 huggingface dataset:
A genomic assessment of the marine-speciation paradox within the toothed whale superfamily Delphinoidea
Authors: Westbury Michael V , Cabrera Andrea A. , Rey-Iglesia Alba , De Cahsan Binia , Duchêne David A. , Hartmann Stefanie , Lorenzen Eline D
Published: October 2020

Abstract

The importance of post-divergence gene flow in speciation has been documented across a range of taxa in recent years, and may have been especially widespread in highly mobile, wide-ranging marine species, such as cetaceans. Here, we studied individual genomes from nine species across the three families of the toothed whale superfamily Delphinoidea (Delphinidae, Phocoenidae, Monodontidae). To investigate the role of post-divergence gene flow in the speciation process, we used a multifaceted approach, including: (i) phylogenomics, (ii) the distribution of shared derived alleles, and (iii) demographic inference. We found the divergence of lineages within Delphinoidea did not follow a process of pure bifurcation, but was much more complex. Sliding-window phylogenomics reveal a high prevalence of discordant topologies within the superfamily, with further analyses indicating these discordances arose due to both incomplete lineage sorting and gene flow. D-statistics, D-foil, andf-branch analyses supported gene flow between members of Delphinoidea, with the vast majority of gene flow occurring as ancient interfamilial events. Demographic analyses provided evidence that introgressive gene flow has likely ceased between all species pairs tested, despite reports of contemporary interspecific hybrids. Our study provides the first steps towards resolving the large complexity of speciation within Delphinoidea; we reveal the prevalence of ancient interfamilial gene flow events prior to the diversification of each family, and suggests that contemporary hybridisation events may be disadvantageous, as hybrid individuals do not appear to contribute to the parental species’ gene pools.
Distinct cellular origins and differentiation process account for distinct oncogenic and clinical behaviors of leiomyosarcomas
Authors: Darbo Elodie , Pérot Gaëlle , Darmusey Lucie , Guellec Sophie Le , Leroy Laura , Gaston Laëtitia , Desplat Nelly , Thébault Noémie , Merle Candice , Rochaix Philippe , Valentin Thibaud , Ferron Gwenaël , Chevreau Christine , Bui Binh , Stoeckle Eberhard , Ranchere-Vince Dominique , Méeus Pierre , Terrier Philippe , Piperno-Neumann Sophie , Collin Françoise , Pinieux Gonzague De , Duffaud Florence , Coindre Jean-Michel , Blay Jean-Yves , Chibon Frédéric
Published: October 2020

In leiomyosarcoma (LMS), a very aggressive disease, a relatively transcriptionally uniform subgroup of well differentiated tumors has been described and is associated with poor survival. The question raised how differentiation and tumor progression, two apparently antagonist processes, coexist and allow tumor malignancy. We first identified the most transcriptionally homogeneous LMS subgroup in three independent cohorts, which we named ‘hLMS’. The integration of multi-omics data and functional analysis suggests that hLMS originate from vascular smooth muscle cells and show that hLMS transcriptional program reflects both modulation of smooth muscle contraction activity controlled by MYOCD/SRF regulatory network and activation of the cell cycle activity controlled by E2F/RB1 pathway. We propose that the phenotypic plasticity of vascular smooth muscle cells coupled with MYOCD/SRF pathway amplification, essential for hLMS survival, concomitant with PTEN absence andRB1alteration, could explain how hLMS balance this uncommon interplay between differentiation and aggressiveness.
Learning sparse codes from compressed representations with biologically plausible local wiring constraints
Authors: Fallah Kion , Willats Adam A. , Liu Ninghao , Rozell Christopher J.
Published: October 2020

Sparse coding is an important method for unsupervised learning of task-independent features in theoretical neuroscience models of neural coding. While a number of algorithms exist to learn these representations from the statistics of a dataset, they largely ignore the information bottlenecks present in fiber pathways connecting cortical areas. For example, the visual pathway has many fewer neurons transmitting visual information to cortex than the number of photoreceptors. Both empirical and analytic results have recently shown that sparse representations can be learned effectively after performing dimensionality reduction with randomized linear operators, producing latent coefficients that preserve information. Unfortunately, current proposals for sparse coding in the compressed space require a centralized compression process (i.e., dense random matrix) that is biologically unrealistic due to local wiring constraints observed in neural circuits. The main contribution of this paper is to leverage recent results on structured random matrices to propose a theoretical neuroscience model of randomized projections for communication between cortical areas that is consistent with the local wiring constraints observed in neuroanatomy. We show analytically and empirically that unsupervised learning of sparse representations can be performed in the compressed space despite significant local wiring constraints in compression matrices of varying forms (corresponding to different local wiring patterns). Our analysis verifies that even with significant local wiring constraints, the learned representations remain qualitatively similar, have similar quantitative performance in both training and generalization error, and are consistent across many measures with measured macaque V1 receptive fields.
Epigenetics and expression of key genes associated with cardiac fibrosis:NLRP3, MMP2, MMP9, CCN2/CTGF, andAGT
Authors: Chandra Sruti , Ehrlich Kenneth C. , Lacey Michelle , Baribault Carl , Ehrlich Melanie
Published: October 2020

Excessive inflammatory signaling and pathological remodeling of the extracellular matrix are important contributors to cardiac fibrosis and involve major changes in gene expression. We examined the relationships between tissue-specific expression and the epigenetics of five genes involved in these pathways,NLRP3, MMP2, MMP9, CCN2/CTGF,andAGT.The proteins encoded by these genes play major fibrosis-related roles in inflammasome formation, degradation of extracellular matrix proteins and remodeling of the extracellular matrix and vasculature, autocrine regulation of fibrosis, or cell signaling. Our analyses showed that the first four of these genes had super-enhancers (unusually strong enhancer clusters) that correlate with their very high expression in monocytes, neutrophils, fibroblasts, or venous cells. Expression of the gene encoding miR-223, a micro-RNA that plays an important role in downregulating NLRP3 protein levels, is also probably driven by the super-enhancer in which it is embedded. Enhancer chromatin for all these genes was inside as well as outside the gene body. WhileAGT,which encodes precursors of angiotensin II, lacked a super-enhancer, its tissue-specific expression profile correlates with the tissue-specific enhancer chromatin extending into its distant silent gene neighbor (CAPN9). Tissue-specific peaks of DNA hypomethylation, open chromatin (DNaseI hypersensitivity), and transcription factor binding were detected in subregions of these super-enhancers/enhancers that are likely to be the main drivers of expression of their associated gene. We found thatCCN2/CTGFis co-expressed with its far-upstream neighborLINC01013, a noncoding RNA gene, specifically in vein endothelial cells (HUVEC) and chondrocytes. Evidence from chromatin looping profiling (Hi-C) suggests coregulation of these genes in HUVEC. Our findings indicate the importance of understanding the often-overlooked roles of enhancers and their hypomethylated, transcription factor-binding subregions in the regulation of expression of fibrosis-related genes in normal and fibrotic tissue.
A derivative of the D5 monoclonal antibody that targets the gp41 N-heptad repeat of HIV-1 with broad tier-2 neutralizing activity
Authors: Rubio Adonis A. , Filsinger Interrante Maria V. , Bell Benjamin N. , Brown Clayton L. , LaBranche Celia C. , Montefiori David C. , Kim Peter S.
Published: October 2020

HIV-1 infection is initiated by the viral glycoprotein Env, which, after interaction with cellular coreceptors, adopts a transient conformation known as the pre-hairpin intermediate (PHI). The N-heptad repeat (NHR) is a highly conserved region of gp41 exposed in the PHI; it is the target of the FDA-approved drug enfuvirtide and of neutralizing monoclonal antibodies (mAbs). However, to date these mAbs have only been weakly effective against tier-1 HIV-1 strains, which are most sensitive to neutralizing antibodies. Here, we engineered and tested 11 IgG variants of D5, an anti-NHR mAb, by recombining previously described mutations in four of D5’s six antibody complementarity-determining regions. One variant, D5_AR, demonstrated 6-fold enhancement in ID50against lentivirus pseudotyped with HXB2 Env. Importantly, D5_AR exhibited weak cross-clade neutralizing activity against a diverse set of tier-2 HIV-1 viruses, which are less sensitive to neutralizing antibodies than tier-1 viruses and are the target of current antibody-based vaccine efforts. In addition, the neutralization potency of D5_AR IgG was greatly enhanced in target cells expressing FcγRI with ID50values below 0.1 μg/mL; this immunoglobulin receptor is expressed on macrophages and dendritic cells, which are implicated in the early stages of HIV-1 infection of mucosal surfaces. D5 and D5_AR have equivalent neutralization potency in IgG, Fab, and scFv formats, indicating that neutralization is not impacted by steric hindrance. Taken together, these results provide support for vaccine strategies that target the PHI by eliciting antibodies against the gp41 NHR.

Importance

Despite advances in anti-retroviral therapy, HIV remains a global epidemic and has claimed more than 32 million lives. Accordingly, developing an effective vaccine remains an urgent public health need. The gp41 N-heptad repeat (NHR) of the HIV-1 pre-hairpin intermediate (PHI) is highly conserved (>90%) and is inhibited by the FDA-approved drug enfuvirtide, making it an attractive vaccine target. However, to date NHR antibodies have not been potent. Here, we engineered D5_AR, a more potent variant of the anti-NHR antibody D5, and established its ability to inhibit HIV-1 strains that are more difficult to neutralize and are more representative of circulating strains (tier-2 strains). The neutralizing activity of D5_AR was greatly potentiated in cells expressing FcγRI; FcγRI is expressed on cells that are implicated at the earliest stages of sexual HIV-1 transmission. Taken together, these results bolster efforts to target the gp41 NHR and the PHI for vaccine development.
CSF-1 maintains pathogenic but not homeostatic myeloid cells in the central nervous system during autoimmune neuroinflammation
Authors: Hwang Daniel , Seyedsadr Maryam S. , Ishikawa Larissa Lumi Watanabe , Boehm Alexandra , Sahin Ziver , Casella Giacomo , Jang Soohwa , Gonzalez Michael V. , Garifallou James P. , Hakonarson Hakon , Zhang Weifeng , Xiao Dan , Rostami Abdolmohamad , Zhang Guang-Xian , Ciric Bogoljub
Published: October 2020

The receptor for colony stimulating factor 1 (CSF-1R) is important for the survival and function of myeloid cells that mediate pathology during experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). CSF-1 and IL-34, the ligands of CSF-1R, have similar bioactivities but distinct tissue and context-dependent expression patterns, suggesting that they have different roles. This could be the case in EAE, given that CSF-1 expression is upregulated in the CNS, while IL-34 remains constitutively expressed. We found that targeting CSF-1 with neutralizing mAb halted ongoing EAE, with efficacy superior to CSF-1R inhibitor BLZ945, whereas IL-34 neutralization had no effect, suggesting that pathogenic myeloid cells were maintained by CSF-1, not IL-34. Both anti-CSF-1- and BLZ945-treatment greatly reduced numbers of monocyte-derived cells and microglia in the CNS. However, anti-CSF-1 selectively depleted inflammatory microglia and monocytes in inflamed CNS areas, whereas BLZ945 depleted virtually all myeloid cells, including quiescent microglia, throughout the CNS. Anti-CSF-1 treatments reduced the size of demyelinated lesions, and microglial activation in the grey matter. Lastly, we found that bone marrow-derived immune cells were the major mediators of CSF-1R-dependent pathology, while microglia played a lesser role. Our findings suggest that targeting CSF-1 could be effective in ameliorating MS pathology, while preserving the homeostatic functions of myeloid cells, thereby minimizing risks associated with total ablation of CSF-1R-dependent cells.

Significance Statement

Multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE), are autoimmune diseases characterized by accumulation of myeloid immune cells into the central nervous system (CNS). Both harmful and beneficial myeloid cells are present in EAE/MS, and a goal of MS therapy is to preferentially remove harmful myeloid cells. The receptor for CSF-1 (CSF-1R) is found on myeloid cells and it is important for their survival. CSF-1R can bind two ligands, CSF-1 and IL-34, but is unknown whether their functions in EAE/MS differ. We found that blocking CSF-1 depleted only harmful myeloid cells in the CNS and suppressed EAE, whereas blocking IL-34 had no effect. Thus, we propose that blocking CSF-1 could be a novel therapy for MS.
Emergent robustness of bacterial quorum sensing in fluid flow
Authors: Dalwadi Mohit P. , Pearce Philip
Published: October 2020

Bacteria use intercellular signaling, or quorum sensing (QS), to share information and respond collectively to aspects of their surroundings. The autoinducers that carry this information are exposed to the external environment; consequently, they are affected by factors such as removal through fluid flow, a ubiquitous feature of bacterial habitats ranging from the gut and lungs to lakes and oceans. To understand how QS genetic architectures in cells promote appropriate populationlevel phenotypes throughout the bacterial life cycle requires knowledge of how these architectures determine the QS response in realistic spatiotemporally varying flow conditions. Here, we develop and apply a general theory that identifies and quantifies the conditions required for QS activation in fluid flow by systematically linking cell- and population-level genetic and physical processes. We predict that, when a subset of the population meets these conditions, cell-level positive feedback promotes a robust collective response by overcoming flow-induced autoinducer concentration gradients. By accounting for a dynamic flow in our theory, we predict that positive feedback in cells acts as a low-pass filter at the population level in oscillatory flow, allowing a population to respond only to changes in flow that occur over slow enough timescales. Our theory is readily extendable, and provides a framework for assessing the functional roles of diverse QS network architectures in realistic flow conditions.
Spaceflight decelerates the epigenetic clock orchestrated with a global alteration in DNA methylome and transcriptome in the mouse retina
Authors: Chen Zhong , Stanbouly Seta , Nishiyama Nina C. , Chen Xin , Delp Michael D. , Qiu Hongyu , Mao Xiao W. , Wang Charles
Published: October 2020

Astronauts exhibit an assortment of clinical abnormalities in their eyes during long-duration spaceflight. The purpose of this study was to determine whether spaceflight induces epigenomic and transcriptomic reprogramming in the retina or alters the epigenetic clock. The mice were flown for 37 days in animal enclosure modules on the International Space Station; ground-based control animals were maintained under similar housing conditions. Mouse retinas were isolated and both DNA methylome and transcriptome were determined by deep sequencing. We found that a large number of genes were differentially methylated with spaceflight, whereas there were fewer differentially expressed genes at the transcriptome level. Several biological pathways involved in retinal diseases such as macular degeneration were significantly altered. Our results indicated that spaceflight decelerated the retinal epigenetic clock. This study demonstrates that spaceflight impacts the retina at the epigenomic and transcriptomic levels, and such changes could be involved in the etiology of eye-related disorders among astronauts.
FloridaDrosophila melanogastergenomes sampled 13 years apart show increases in warm-associated SNP alleles
Authors: Veeramah Krishna R. , Brud Evgeny , Eanes Walter F.
Published: October 2020

We studied genetic change inDrosophila melanogasterusing whole-genome SNP data from samples taken 13 years apart in Homestead, FL. This population is at the southern tip of a well-studied US latitudinal cline. On the non-inversion-carrying chromosome arms, 11-16% of SNPs show significant frequency changes. These are enriched for latitudinal clines and genic sites. For clinal SNPs each allele is either the northern- or southern-favored. Seventy-eight to 95 percent with significant frequency increase are southern-favored. Five to seven percent of SNPs also show significant seasonal change and involve increases in the northern-favored allele during the season. On the 2L and 3R chromosome arms there are significant seasonal shifts for common inversions. We identify regions and genes that are candidates for selection. These regions also show correspondence with those associated with soft sweeps in Raleigh, NC. This shift towards southern-favored alleles may be caused by climate shifts or increased African-European admixture.
Pharmacological but not physiological GDF15 suppresses feeding and the motivation to exercise
Authors: Klein Anders B. , Nicolaisen Trine S. , Ørtenblad Niels , Gejl Kasper D. , Jensen Rasmus , Fritzen Andreas M. , Larsen Emil L. , Karstoft Kristian , Poulsen Henrik E. , Morville Thomas , Sahl Ronni E. , Helge Jørn W. , Lund Jens , Falk Sarah , Lyngbæk Mark , Ellingsgaard Helga , Pedersen Bente K. , Lu Wei , Finan Brian , Jørgensen Sebastian B. , Seeley Randy J. , Kleinert Maximilian , Kiens Bente , Richter Erik A. , Clemmensen Christoffer
Published: October 2020

Growing evidence supports that pharmacological application of growth differentiation factor 15 (GDF15) suppresses appetite but also promotes sickness-like behaviors in rodents via GDNF family receptor α-like (GFRAL)-dependent mechanisms1,2. Conversely, the endogenous regulation and secretion of GDF15 and its physiological effects on energy homeostasis and behavior remain elusive. Here we show, in four independent studies that prolonged, moderate- to high-intensity endurance exercise substantially increases circulating GDF15, in a time-dependent and reversible fashion, to peak levels otherwise only observed in pathophysiological conditions. This exercise-induced increase can be recapitulated in mice following forced treadmill running and is accompanied by increasedGdf15expression in the liver, skeletal muscle, and heart muscle. Compared to other metabolic stressors, like fasting, acute high-fat diet feeding, severe caloric excess and temperature changes, exercise has a greater impact on circulating GDF15 levels. However, whereas pharmacological GDF15 inhibits appetite and suppresses wheel running activity via GFRAL, in response to exercise, the physiological induction of GDF15 does not. In summary, exercise-induced circulating GDF15 correlates with the duration of endurance exercise. However, higher GDF15 levels after exercise are not sufficient to evoke canonical pharmacological GDF15 effects on appetite or responsible for exercise aversion/fatigue. Thus, the physiological effects of GDF15 as an exerkine remain elusive.
Analysis of the Immune Response to Sciatic Nerve Injury Identifies Efferocytosis as a Key Mechanism of Nerve Debridement
Authors: Kalinski Ashley L. , Yoon Choya , Huffman Lucas D. , Duncker Patrick C. , Kohen Rafi , Passino Ryan , Hafner Hannah , Johnson Craig , Kawaguchi Riki , Carbajal Kevin S. , Jara Juan Sebastián , Hollis Edmund , Geschwind Daniel H. , Segal Benjamin M. , Giger Roman J.
Published: October 2020

Sciatic nerve crush injury triggers sterile inflammation within the distal nerve and axotomized dorsal root ganglia (DRGs). Granulocytes and pro-inflammatory Ly6Chighmonocytes infiltrate the nerve first, and rapidly give way to Ly6Cnegativeinflammation-resolving macrophages. In axotomized DRGs, few hematogenous leukocytes are detected and resident macrophages acquire a ramified morphology. Single-cell RNA-sequencing of injured sciatic nerve identifies five macrophage subpopulations, repair Schwann cells, and mesenchymal precursor cells. Macrophages at the nerve crush site are molecularly distinct from macrophages associated with Wallerian degeneration. In the injured nerve, macrophages “eat” apoptotic leukocytes, a process called efferocytosis, and thereby promote an anti-inflammatory milieu. Myeloid cells in the injured nerve, but not axotomized DRGs, strongly express receptors for the cytokine GM-CSF. In GM-CSF deficient (Csf2-/-) mice, inflammation resolution is delayed and conditioning-lesion induced regeneration of DRG neuron central axons is abolished. Thus, carefully orchestrated inflammation resolution in the nerve is required for conditioning-lesion induced neurorepair.
Rapid evolution of enhanced Zika virus virulence during direct vertebrate transmission chains
Authors: Riemersma Kasen K. , Jaeger Anna S. , Crooks Chelsea M. , Braun Katarina M. , Weger-Lucarelli James , Ebel Gregory D. , Friedrich Thomas C. , Aliota Matthew T.
Published: October 2020

Zika virus (ZIKV) has the unusual capacity to circumvent natural alternating mosquito-human transmission and be directly transmitted human-to-human via sexual and vertical routes. The impact of direct transmission on ZIKV evolution and adaptation to vertebrate hosts is unknown. Here we show that molecularly barcoded ZIKV rapidly adapted to a mammalian host during direct transmission chains in mice, coincident with the emergence of an amino acid substitution previously shown to enhance virulence. In contrast, little to no adaptation of ZIKV to mice was observed following chains of direct transmission in mosquitoes or alternating host transmission. Detailed genetic analyses revealed that ZIKV evolution in mice was generally more convergent and subjected to more relaxed purifying selection than in mosquitoes or alternate passages. These findings suggest that prevention of direct human transmission chains may be paramount to resist gains in ZIKV virulence.
Towards designing globular antimicrobial peptide mimics: role of polar functional groups in biomimetic ternary antimicrobial polymers
Authors: Rani Garima , Kuroda Kenichi , Vemparala Satyavani
Published: October 2020

Using atomistic molecular dynamics simulations, we study the interaction of ternary methacrylate polymers, composed of charged cationic, hydrophobic and neutral polar groups, with model bacterial membrane. Our simulation data shows that the random ternary polymers can penetrate deep into the membrane interior and partitioning of even a single polymer has a pronounced effect on the membrane structure. Lipid reorganization, on polymer binding, shows a strong affinity of the ternary polymer for anionic POPG lipids and the same is compared with the control case of binary polymers (only cationic and hydrophobic groups). While binary polymers exhibit strong propensity of acquired amphiphilic conformations upon membrane insertion, our results strongly suggest that such amphiphilic conformations are absent in the case of random ternary polymers. The ternary polymers adopt a more folded conformation, staying aligned in the direction of the membrane normal and subsequently penetrating deeper into the membrane interior suggesting a novel membrane partitioning mechanism without amphiphilic conformations. Finally, we also examine the interactions of ternary polymer aggregates with model bacterial membranes, which show that replacing some of the hydrophobic groups by polar groups leads to weakly held ternary aggregates enabling them to undergo rapid partitioning and insertion into membrane interior. Our work thus underscores the role of inclusion of polar groups into the framework of traditional binary biomimetic antimicrobial polymers and suggests different mode of partitioning into bacterial membranes, mimicking antimicrobial mechanism of globular antimicrobial peptides like Defensin.
Bifidobacteria-mediated immune system imprinting early in life
Authors: Henrick Bethany M. , Rodriguez Lucie , Lakshmikanth Tadepally , Pou Christian , Henckel Ewa , Olin Axel , Wang Jun , Mikes Jaromir , Tan Ziyang , Chen Yang , Ehrlich Amy M. , Bernhardsson Anna Karin , Mugabo Constantin Habimana , Ambrosiani Ylva , Gustafsson Anna , Chew Stephanie , Brown Heather K. , Prambs Johann , Bohlin Kajsa , Mitchell Ryan D. , Underwood Mark A. , Smilowitz Jennifer T. , German J. Bruce , Frese Steven A. , Brodin Petter
Published: October 2020

Immune-microbe interactions early in life influence an individual’s risk of developing allergies, asthma and some autoimmune disorders. Breastfeeding helps guide the development of healthy immune-microbe relationships, in part by providing nutrients to specialized microbes that in turn benefit the host and its developing immune system. Such bacteria having co-evolved with humans are associated with reduced risks of immune mediated diseases but are increasingly rare in modern societies. Here we map an immunological sequence of events, triggered by microbial colonization that distinguish children with different gut bacterial composition. Lack of bifidobacterial species is associated with elevated markers of intestinal inflammation and immune dysregulation and in a randomized trial of breastfed infants, the infant-adaptedBifidobacterium infantisEVC001 silenced intestinal Th2 and Th17 immune responses, while inducing IFNβ, and its metabolites skew T-cell polarizationin vitro, from Th2 towards Th1, suggesting a healthier immune imprinting during the first critical months of life.

HIGHLIGHTS

An ordered sequence of immune changes after birth, driven by microbial interactions

Low gutBifidobacteriumabundance is associated with markers of intestinal inflammation

FeedingB. infantisEVC001 silenced intestinal Th2 and Th17 but upregulates IFNβ

B. infantisEVC001 metabolites and/or enteric cytokines skew naïve T-cell polarization towards Th1 and away from Th2
How does film adaptation influence box office performance? An empirical analysis of science fiction films in Hollywood
Authors: Ryu Sunghan
Published: October 2020

Purpose

This study aims to identify the factors that influence box office performance in the specific context of the adaptation of science fiction (SF) to film in Hollywood.

Design/methodology/approach

Fifty-one film adaptation cases were collected and empirically analyzed with two-stage least-squares (2SLS) regression.

Findings

Empirical analysis demonstrates that the adaptation of the title, the popularity of the original novel and the director's experience in film adaptation have significant impacts on box office performance.

Research limitations/implications

The study contributes to the literature by bridging the gap between two separate streams of the research literature on film performance and film adaptation. Moreover, the study has extended the literature on the prediction of film performance by examining important factors in the special context of SF film adaptation.

Practical implications

In the case of film adaptation, recruiting an experienced director will be a good choice. Author power is also required for attracting more investment and increasing audience share in the short term. From a marketing perspective, pointing out in the title that the film is an adaptation of an original novel would be an advantageous approach.

Originality/value

This is among the pioneering research related to the effects of film adaptation on box office performance. The approach and results of this study direct future studies in many aspects.
Effect of environmental management accounting practices on organizational performance: role of process innovation as a mediating variable
Authors: Sari Ria Nelly , Pratadina Aura , Anugerah Rita , Kamaliah Kamaliah , Sanusi Zuraidah Mohd
Published: October 2020

Purpose

This study aims to determine (1) the effect of environmental management accounting on organizational performance and (2) the mediating effect of process innovation on the relationship between environmental management accounting and organizational performance.

Design/methodology/approach

This research uses survey methods designed for management accountants of large manufacturing companies in Indonesia. Data from 123 respondents were analyzed using the WarpPLS.

Findings

The implementation of environmental management accounting exerted a positive effect on organizational performance. It is evident that the implementation of environmental management accounting encourages companies to innovate processes which will improve how well the organization performs.

Research limitations/implications

These findings still contained several limitations such as data were only collected from one province in Indonesia, and so the findings might not be generalizable to other provinces in Indonesia. Also, the number of variables studied only involved three. The study only focused on certain dimensions of environmental management accounting without considering other dimensions in-depth.

Practical implications

These findings extend the literature on environmental management accounting and demonstrate, from a practical perspective, environmental management accounting (EMA), which prioritizes the environment, will encourage companies to innovate their processes so that they are more environmentally friendly; EMA recognizes the important role of accountants in managing environmental issues.

Originality/value

This study documents the importance of environmental management accounting to assist companies in getting accurate information on environmental issues and environmental costs.
Document delivering services: a strategy to support research in Mexican universities in the pandemic by COVID-19
Authors: Saavedra-Alamillas César , Pacheco-Mendoza Josmel , García-Meléndez Héctor Eduardo , Vilchis López Amado , Martínez-Camacho Humberto , Ortiz-Díaz Erik Miguel , Ortega-Martínez Eugenia
Published: October 2020

Purpose

The purpose of this paper is to report and to regulate the users’ documentary necessities of six Mexican universities involved, in a cooperative manner and during the period of confinement by SARS-CoV-2, through the Document Delivery Service (DDS).

Design/methodology/approach

A diagnostic was conducted within the participating institutions that considered the information resources and sources, documents requested and the number of users per institution, which were counted daily for one month. This research used documentary and quantitative methods.

Findings

The authors determined that DDS is a reliable service for information supply although it is going to be necessary to adjust legal schemes and interlibrary loan policies to develop a process and to adapt this one to the current information services in the future or “in our new normality”; DDS will have a high impact on the conformation of networks of librarian cooperation.

Originality/value

This study exposed the importance of DDS as a relevant service to provide information on demand by using information and communication technologies, which is a safe way to operate, avoiding exposure to physical contact, ensuring the continuity of research processes in the communities and always monitoring and promoting the copyright.
An adaptive resistance and stamina strategy-based dragonfly algorithm for solving engineering optimization problems
Authors: Yuan Yongliang , Wang Shuo , Lv Liye , Song Xueguan
Published: October 2020

Purpose

Highly non-linear optimization problems exist in many practical engineering applications. To deal with these problems, this study aims to propose an improved optimization algorithm, named, adaptive resistance and stamina strategy-based dragonfly algorithm (ARSSDA).

Design/methodology/approach

To speed up the convergence, ARSSDA applies an adaptive resistance and stamina strategy (ARSS) to conventional dragonfly algorithm so that the search step can be adjusted appropriately in each iteration. In ARSS, it includes the air resistance and physical stamina of dragonfly during a flight. These parameters can be updated in real time as the flight status of the dragonflies.

Findings

The performance of ARSSDA is verified by 30 benchmark functions of Congress on Evolutionary Computation 2014’s special session and 3 well-known constrained engineering problems. Results reveal that ARSSDA is a competitive algorithm for solving the optimization problems. Further, ARSSDA is used to search the optimal parameters for a bucket wheel reclaimer (BWR). The aim of the numerical experiment is to achieve the global optimal structure of the BWR by minimizing the energy consumption. Results indicate that ARSSDA generates an optimal structure of BWR and decreases the energy consumption by 22.428% compared with the initial design.

Originality/value

A novel search strategy is proposed to enhance the global exploratory capability and convergence speed. This paper provides an effective optimization algorithm for solving constrained optimization problems.
Cultivating interdisciplinary team creativity through an intensive design competition
Authors: Han Soo Jeoung , Abadi Mehrangiz , Jin Bora , Chen Jie
Published: October 2020

Purpose

The authors examined team-learning processes in short-term student project teams operating in an intensive design competition at a public university. The purpose of this paper is to explore the critical facilitators, inhibitors and processes for fostering students' creativity within interdisciplinary design teams in higher education.

Design/methodology/approach

The authors used a qualitative design to explore facilitators, inhibitors and critical processes in interdisciplinary student project teams. They conducted focus group interviews with three winning interdisciplinary teams that participated in a three-day design competition and used a constant comparison to analyze the data.

Findings

The authors identified themes that contributed to creativity at the individual level, the team level and the resource level. The key findings included 12 critical team process phases to achieve one common goal.

Originality/value

The findings of the study yielded to a holistic model of interdisciplinary team development for creativity. Implications for educators and practitioners and suggestions for researchers to expand the interdisciplinary team process model were discussed to facilitate interdisciplinary team creativity in higher education.
Association of patient safety indicator 03 and clinical outcome in a surgery hospital
Authors: Al-Tehewy Mahi Mahmoud , Abd Al-Razak Sara Ebraheem , Hikal Tamer Shahat , Wahdan Maha Magdy
Published: October 2020

Purpose

Patient safety indicators (PSIs) were developed as a tool for hospitals to identify potentially preventable complications and improve patient safety performance. The study aimed at measuring the incidence of the Agency for Healthcare Research and Quality (AHRQ) PSI03 (pressure ulcer [PU] rate) and to identify the association between PSI03 and clinical outcomes including death, readmission within 30 days and length of stay (LOS) at the cardiothoracic surgery hospital at Ain Shams University, Cairo, Egypt.

Design/methodology/approach

An exploratory prospective cohort study was conducted to follow up patients, who fulfilled the inclusion criteria, from admission until one month after discharge at the cardiothoracic surgery hospital. Data were collected through basic information and follow-up sheets. The total number of included participants in the study was 330.

Findings

PSI03 incidence rate was 67.7 per 1,000 discharges. Patients aged 60 years and above had the highest risk among all age groups. In patients who developed PSI03, the risk ratio (RR) of death was 8.8 [95% CI (3.79–20.24)], RR of staying more than 30 days at the hospital was 1.5 [95% CI (1.249–1.872)] and of readmission within 30 days in patients who developed PSI03 was 1.5 [95% CI (0.38–6.15)]. In the study’s hospital, the patients who developed PSI03 were at higher risk of death and stayed longer at the hospital than patients without PSI03. This study demonstrated a clear association between PSI03 and patient outcomes such as LOS and mortality. Early detection, prevention and proper management of PSI03 are recommended to decrease unfavorable clinical outcomes.

Originality/value

The importance of PSIs lies in the fact that they facilitate the recognition of the adverse events and complications which occurred during hospitalization and give the hospitals a chance to improve the possible clinical outcomes. Therefore, the current study aimed at measuring the association between AHRQ PSI03 ( PU rate) and the clinical outcomes including death, readmission within 30 days and the LOS at the cardiothoracic surgery hospital at Ain Shams University. This study will provide the hospital management with baseline data for this type of adverse event and guide them to develop a system for identifying the high-risk group of patients and to upgrade relevant hospital policies and guidelines that lead to improved patient outcomes.
Satisfaction with work and person–environment fit: are there intergenerational differences? An examination through person–job, person–group and person–supervisor fit
Authors: Deschênes Andrée-Anne
Published: October 2020

Purpose

Through three dimensions of person–environment (PE) fit, namely person–job (PJ) fit, person–group (PG) fit and person–supervisor (PS) fit, this paper examines generational differences on which dimension is more important to explain Baby Boomers', Generation X's and Generation Y's satisfaction with work.

Design/methodology/approach

Gathered from a sample of 1,065 employees in the province of Québec, Canada, data were analyzed through one-way ANOVA and structural equation modeling.

Findings

The findings suggest that Generation X scored lower on satisfaction with work, that there is a difference in the level of PG fit and PS fit between the generations, and that PJ fit explains satisfaction with work for all generations, while PG fit is significant only for Generation Y employees.

Practical implications

This paper sheds light on the importance for practitioners, when implementing human resource (HR) policies and strategies aiming to increase satisfaction with work, of prioritizing PJ fit and to consider PG fit for Generation Y members.

Originality/value

This research provides a meaningful contribution to current knowledge on generational diversity in the workplace and its impact on managerial practices by examining different levels of satisfaction with work and of PJ, PG and PS fit for three generations and the importance of each type of fit in explaining satisfaction with work for theses generations.
Accurately or accidentally? Recommendation agent and search experience in over-the-top (OTT) services
Authors: Kwon Yeeun , Park Jaecheol , Son Jai-Yeol
Published: October 2020

Purpose

Over-the-top (OTT) services, which provide streaming media through all devices in online setting, have surpassed the traditional content providers in the market. However, there is still no clear empirical evidence that indicates what recommendation agent values affect the users' search experience while using the OTT services and how it leads to continuous subscription. To address this gap, this study aims to examine recommendation agent values influencing search experience, which in turn affects decision satisfaction and continuance intention.

Design/methodology/approach

This study empirically develops and tests a research model with data obtained from 212 survey respondents in Korea. Structural equation modeling with partial least square approach was used to analyze the data.

Findings

(1) Recommendation agent variables such as match score accuracy, recommended content variety and thumbnail image appeal affect search experience variables such as perceived diagnosticity and perceived serendipity; (2) perceived diagnosticity and perceived serendipity of search experience increase decision satisfaction; and (3) decision satisfaction increases intention to continue to subscribe to OTT services.

Originality/value

Despite the widespread use of recommendation agents in OTT services, limited attention has been paid to understand what specific values of recommendation agents lead subscribers to continue their subscription. The findings of this study clarify subscribers' continuous subscription behavior in OTT services in terms of the recommendation agent values and search experience perspective.
Impact of personality traits and university green entrepreneurial support on students' green entrepreneurial intentions: the moderating role of environmental values
Authors: Qazi Wasim , Qureshi Jawaid Ahmed , Raza Syed Ali , Khan Komal Akram , Qureshi Muhammad Asif
Published: October 2020

Purpose

The earth needs to go green as it clarifies that humans should adopt a sustainable lifestyle that will be friendly to the environment and society. The emerging stream of academic literature on “go green” adds a new perspective to the deep-rooted conception of entrepreneurship. The objective of green entrepreneurship is to follow the traditional concepts of entrepreneurship but also brings the additional potential for society and the environment. The present paper is designed to study the impact of personality traits and university green entrepreneurial support on green entrepreneurial intentions along with the moderating role of environmental values.

Design/methodology/approach

The data are collected by using the survey method, and the five-point Likert scale is used for this purpose. The statistical techniques applied to the dataset were confirmatory factor analysis and partial least square structural equation modeling.

Findings

The results revealed that personality traits are positively and significantly associated with green entrepreneurial intention, but risk-averse students depict insignificant results towards green entrepreneurial intention. Also, university green entrepreneurial support results in positive green entrepreneurial intention. Environmental values also strengthen the association.

Originality/value

Previously, researchers emphasized the entrepreneurial intentions for the sake of improving the economy, decreasing unemployment and promoting small ventures, but none focused on the green aspects of entrepreneurship. To date, we still lack knowledge regarding students' green entrepreneurial intentions and how personality traits and green support affect the intention. The focus of the present study is to sustain nature and ecosystems through green entrepreneurship by providing directions and economic and noneconomic gains for investors, entrepreneurs, students, universities and societies.
Knowledge grafting during internationalization: utilizing localized professionals in the foreign market
Authors: Johanson Martin , Kao Pao T. , Lundberg Heléne
Published: October 2020

Purpose

The purpose of this paper is to understand knowledge grafting through localized professionals in the internationalization of the firm. Knowledge grafting refers to firms increasing their knowledge stock by acquiring new staff, and while the concept is not new in studies on firms’ internationalization, there is little understanding of the characteristics of the individuals carrying the knowledge, the types of knowledge grafted and how it contributes to a market entry process.

Design/methodology/approach

The authors conducted an explorative study with a multiple-case research design and purposely selected five localized Swedish managers working for Russian subsidiaries of Swedish firms. Face-to-face interviews were conducted. The interviews were transcribed and analyzed based on three types of knowledge: general foreign market knowledge, social network knowledge and professional knowledge. The authors also considered both private and professional ties.

Findings

The findings show that characteristics of the localized professional and the firm can influence the type of knowledge grafted and how it is used. The findings also highlight the key role of the individual as knowledge carrier and show an alternative way to obtain knowledge in firm internationalization.

Research limitations/implications

This study comes with limitations. Only Swedish firms entering Russia with wholly owned subsidiaries have been considered. Further studies comparing knowledge grafting with firms in different entry mode, varying stage of market entry, as well as other countries of origin can further enrich our understanding. Future studies can also focus on localized professionals to shed light on the knowledge transfer between them and other individuals within the firms and the potential impact of their departure on knowledge grafting.

Practical implications

Internationalizing firms should pay attention to the opportunity of grafting knowledge by appointing localized professionals already living in the market. Governmental agencies in the host county can be a valuable source for identifying foreign nationals of the same origin as the firm.

Originality/value

To the best of the authors’ knowledge, this is the first study to focus on the individual level of knowledge grafting and to examine how localized professionals acquire knowledge to support firms in internationalization.
Mobile contextual marketing in a museum setting
Authors: Dou Xueting , Fan Alei , Cai Liping
Published: October 2020

Purpose

This paper aims to investigate how the contextual marketing strategy facilitated by mobile technology enhances consumers’ purchase intention and experiential quality in a hedonic service setting – museum. Specifically, this study explores the impacts of a context-triggered push notification promoting context-related souvenirs embedded in the museum mobile app on visitors’ souvenir purchase intention and visit experience.

Design/methodology/approach

This study adopted a scenario-based experimental design with video stimuli and a follow-up survey questionnaire. A total of 151 participants were randomly assigned to one of the two art museum visiting scenarios (i.e. with vs without the in-app push notification). A series of one-way ANOVA comparisons and serial mediation tests were conducted for hypotheses testing.

Findings

The results show that the mobile app promoting context-related souvenirs positively affects museum visitors’ souvenir purchase intention without undermining their experiential quality. The serial mediation effects through contextual perceived value and impulse buying tendency further explain how the in-app push notification influences visitors’ souvenir purchase intention.

Originality/value

This study extends the contextual marketing research to a unique service domain (i.e. museum) and contributes to the service technology literature. The findings present empirical evidence for the effectiveness of mobile contextual marketing and its potential to enhance consumers’ experiential quality in a hedonic service setting. The current research provides practical guidelines to both the museum management and mobile app developers.