bluuebunny/crossref_metadata_2025 huggingface dataset:
Polarization and Spectral Energy Distribution in OJ 287 during the 2016/17 Outbursts
Authors: Valtonen Mauri , Zola Stanislaw , Jermak Helen , Ciprini Stefano , Hudec Rene , Dey Lankeswar , Gopakumar Achamveedu , Reichart Daniel L. , Caton Daniel B. , Gazeas Kosmas , Matsumoto Katsura , Ogloza Waldemar , Drozdz Marek , Alicavus Fahri , Baransky Oleksandr , Berdyugin Andrei , Boumis Panos , Bufan Yurii , Debski Bartlomiej , Er Huseyin , Erdem Ahmet , Godunova Vira , Haque Shirin , Hoette Vivian L. , Janik Jan , Kidger Mark , Kundera Tomasz , Kurowski Sebastian , Liakos Alexis , Mohammed Isa , Nilsson Kari , Pajdosz Urszula , Piirola Vilppu , Pursimo Tapio , Rajkumar Brandon , Simon Andrii O. , Siwak Michal , Sonbas Eda , Steele Ian A. , Vasylenko Volodymir V. , Zejmo Michal , Zielinski Pawel
Published: November 2017

We report optical photometric and polarimetric observations of the blazar OJ 287 gathered during 2016/17. The high level of activity, noticed after the General Relativity Centenary flare, is argued to be part of the follow-up flares that exhibited high levels of polarization and originated in the primary black hole jet. We propose that the follow-up flares were induced as a result of accretion disk perturbations, travelling from the site of impact towards the primary SMBH. The timings inferred from our observations allowed us to estimate the propagation speed of these perturbations. Additionally, we make predictions for the future brightness of OJ 287.
Micromechanical Characterization of Hydrogels Undergoing Swelling and Dissolution at Alkaline pH
Authors: Hu Wei , Martin Francois , Jeantet Romain , Chen Xiao , Mercadé-Prieto Ruben
Published: November 2017

The swelling of polyelectrolyte hydrogels usually depends on the pH, and if the pH is high enough degradation can occur. A microindentation device was developed to dynamically test these processes in whey protein isolate hydrogels at alkaline pH 7–14. At low alkaline pH the shear modulus decreases during swelling, consistent with rubber elasticity theory, yet when chemical degradation occurs at pH ≥ 11.5 the modulus decreases quickly and extensively. The apparent modulus was constant with the indentation depth when swelling predominates, but gradients were observed when fast chemical degradation occurs at 0.05–0.1 M NaOH. In addition, these profiles were constant with time when dissolution rates are also constant, the first evidence that a swollen layer with steady state mechanical properties is achieved despite extensive dissolution. At >0.5 M NaOH, we provide mechanical evidence showing that most interactions inside the gels are destroyed, gels were very weak and hardly swell, yet they still dissolve very slowly. Microindentation can provide complementary valuable information to study the degradation of hydrogels.
Effect of Shear History on Rheology of Time-Dependent Colloidal Silica Gels
Authors: Santos Paulo , Carignano Marcelo , Campanella Osvaldo
Published: November 2017

This paper presents a rheological study describing the effects of shear on the flow curves of colloidal gels prepared with different concentrations of fumed silica (4%, 5%, 6%, and 7%) and a hydrophobic solvent (Hydrocarbon fuel, JP-8). Viscosity measurements as a function of time were carried out at different shear rates (10, 50, 100, 500, and 1000 s−1), and based on this data, a new structural kinetics model was used to describe the system. Previous work has based the analysis of time dependent fluids on the viscosity of the intact material, i.e., before it is sheared, which is a condition very difficult to achieve when weak gels are tested. The simple action of loading the gel in the rheometer affects its structure and rheology, and the reproducibility of the measurements is thus seriously compromised. Changes in viscosity and viscoelastic properties of the sheared material are indicative of microstructural changes in the gel that need to be accounted for. Therefore, a more realistic method is presented in this work. In addition, microscopical images (Cryo-SEM) were obtained to show how the structure of the gel is affected upon application of shear.
Gene-Based Pathogen Detection: Can We Use qPCR to Predict the Outcome of Diagnostic Metagenomics?
Authors: Andersen Sandra , Fachmann Mette , Kiil Kristoffer , Møller Nielsen Eva , Hoorfar Jeffrey
Published: November 2017

In microbial food safety, molecular methods such as quantitative PCR (qPCR) and next-generation sequencing (NGS) of bacterial isolates can potentially be replaced by diagnostic shotgun metagenomics. However, the methods for pre-analytical sample preparation are often optimized for qPCR, and do not necessarily perform equally well for qPCR and sequencing. The present study investigates, through screening of methods, whether qPCR can be used as an indicator for the optimization of sample preparation for NGS-based shotgun metagenomics with a diagnostic focus. This was used on human fecal samples spiked with 103 or 106 colony-forming units (CFU)/g Campylobacter jejuni, as well as porcine fecal samples spiked with 103 or 106 CFU/g Salmonella typhimurium. DNA was extracted from the samples using variations of two widely used kits. The following quality parameters were measured: DNA concentration, qPCR, DNA fragmentation during library preparation, amount of DNA available for sequencing, amount of sequencing data, distribution of data between samples in a batch, and data insert size; none showed any correlation with the target ratio of the spiking organism detected in sequencing data. Surprisingly, diagnostic metagenomics can have better detection sensitivity than qPCR for samples spiked with 103 CFU/g C. jejuni. The study also showed that qPCR and sequencing results may be different due to inhibition in one of the methods. In conclusion, qPCR cannot uncritically be used as an indicator for the optimization of sample preparation for diagnostic metagenomics.
Differentially Expressed tRNA-Derived Small RNAs Co-Sediment Primarily with Non-Polysomal Fractions in Drosophila
Authors: Göktaş Çağdaş , Yiğit Hatice , Coşacak Mehmet İlyas , Akgül Bünyamin
Published: November 2017

Recent studies point to the existence of poorly characterized small regulatory RNAs generated from mRNAs, rRNAs and tRNAs. To explore the subcellular location of tRNA-derived small RNAs, 0–1 and 7–8 h Drosophila embryos were fractionated on sucrose density gradients. Analysis of 12,553,921 deep-sequencing reads from unfractionated and fractionated Drosophila embryos has revealed that tRFs, which are detected mainly from the 5’ends of tRNAs, co-sediment with the non-polysomal fractions. Interestingly, the expression levels of a subset of tRFs change temporally following the maternal-to-zygotic transition in embryos. We detected non-polysomal association of tRFs in S2 cells as well. Differential tRF expression pattern points to developmental significance at the organismal level. These results suggest that tRFs are associated primarily with the non-polysomal complexes in Drosophila embryos and S2 cells.
Wood Petrifaction: A New View of Permineralization and Replacement
Authors: Mustoe George E.
Published: November 2017

Petrified wood has traditionally been divided into two categories based on preservation processes: permineralization (where tissues are entombed within a mineral-filled matrix) and replacement (where organic anatomical features have been replicated by inorganic materials). New analytical evidence suggests that for most petrified wood, permineralization and replacement are not independent processes; instead, both processes may occur contemporaneously during diagenesis. Infiltration of mineral-bearing groundwater may initially cause permineralization of cellular tissues, but the wood is undergoing gradual degradation. The degree of anatomical preservation thus depends on the relative rates of mineral precipitation and tissue destruction. Rapid rates of mineralization under relatively mild Eh and pH conditions favor the preservation of organic matter. These conditions appear to be more common for calcium carbonate deposition than for silicification, based on observations of fossil woods from many localities. Because of these preservational complexities, “mineralization” and “mineralized” are more accurate as general descriptive terms than “permineralization” and “permineralized”.
Real-Time Three-Dimensional Imaging of Soil Resistivity for Assessment of Moisture Distribution for Intelligent Irrigation
Authors: Tremsin Vasily
Published: November 2017

An affordable standalone sensor that can provide volumetric information on soil moisture distribution in real time was developed and tested for potential application in irrigation control systems. The moisture content of soil is reconstructed tomographically from electrical resistivity measured between multiple pairs of electrodes, which are installed on two opposite sides of the soil volume. The measurement of relative moisture content reconstructed from the measured resistance values demonstrated in this paper requires a simple, in-situ, two-point calibration (for dry and wet soil conditions) after electrodes are installed in place. This calibration has to be repeated once the soil conditions, such as salinity or fertilizer content, are altered as the season progresses. Historical data collected over a 12-month period can be stored locally or transferred over a wireless network at given intervals or in real time. Although existing single-point sensors can provide more accurate measurements of soil moisture, knowledge on the three-dimensional distribution of moisture around plant roots should allow substantial savings of precious fresh water resources and more intelligent multi-channel irrigation systems. The same system can possibly be extended to estimation of fertilizer distribution.
Dual Competing Photovoltaic Supply Chains: A Social Welfare Maximization Perspective
Authors: Chen Zhisong , Su Shong-Iee
Published: November 2017

In the past decades, the inappropriate subsidy policies in many nations have caused problems such as serious oversupply, fierce competition and subpar social welfare in the photovoltaic (PV) industry in many nations. There is a clear shortage in the PV industry literature regarding how dual supply chains compete and the key decision issues regarding the competition between dual PV supply chains. It is critical to develop effective subsidy policies for the competing PV supply chains to achieve social welfare maximization. This study has explored the dual PV supply chain competition under the Bertrand competition assumption by three game-theoretical modeling scenarios (or supply chain strategies) considering either the public subsidy or no subsidy from a social welfare maximization perspective. A numerical analysis complemented by two sensitivity analyses provides a better understanding of the pricing and quantity decision dynamics in the dual supply chains under three different supply chain strategies and the corresponding outcomes regarding the total supply chain profits, the social welfare and the required total subsidies. The key findings disclose that if there are public subsidies, the dual PV supply chains have the strongest intention to pursue the decentralized strategy to achieve their maximal returns rather than the centralized strategy that would achieve the maximal social welfare; however, the government would need to pay for the maximal subsidy budget. Thus, the best option for the government would be to encourage the dual PV supply chains to adopt a centralized strategy since this will not only maximize the social welfare but also, at the same time, minimize the public subsidy. With a smart subsidy policy, the PV industry can make the best use of the subsidy budget and grow in a sustainable way to support the highly demanded solar power generation in many countries trying very hard to increase the proportion of their clean energy to combat the global warming effect. Several subsidy policies such as shared solar energy arrangements and performance-based incentive (PBI) are proposed to integrate the market users and the PV supply chains. This study serves as a pioneering study into the dual PV supply chain research which is very limited in the PV management and policy study literature. The findings and several untended issues provide a foundation for the future PV supply chain studies.
Clinical Beneficial Effects of Using Crystalloid only in Recipients of Living Donor Liver Transplantation
Authors: Huang Chia-Jung , Cheng Kwok-Wai , Chen Chao-Long , Wu Shao-Chun , Shih Tsung-Hsiao , Yang Sheng-Chun , Juang Sin-Ei , Lee Ying-En , Huang Chiu-En , Jawan Bruno , Wang Chih-Hsien
Published: November 2017

Objective: Liver transplantation (LT) is a major surgery associated with intraoperative massive fluid shift, which is usually replaced by crystalloid, 5% albumin (colloid) and blood products. We studied 15 patients from 477 consecutive recipients of adult living donor liver transplantation. Each patient received crystalloid only during LT. Whether LT provides any clinical benefit is not clear and must be determined. Methods and Patients: The anesthesia records of 477 adult LDLT were reviewed retrospectively. The patients were divided into three groups according to the fluids received. Group I (GI) had received blood products, 5% albumin and crystalloid, group II (GII) received 5% albumin and crystalloid, and group III (GIII) received crystalloid only. The characteristic intraoperative variable and postoperative acute rejection and survival rate were compared amongst groups by using One Way ANOVA post hoc with Bonferroni and by Ficher’s Exact test and Chi-square χ2 test. Results and Conclusions: GIII had less intraoperative ascites and blood loss; they also had more stable hemodynamics. Furthermore, they could be extubated significantly earlier than GI, and the one- and three-year survival rates were excellent, with 100% in GIII, while that of GI and GII were 94.1%, 90.5% and 98.6%, 94.5%, respectively.
The Short-Term Effects of Visibility and Haze on Mortality in a Coastal City of China: A Time-Series Study
Authors: Gu Shaohua , Yang Jun , Woodward Alistair , Li Mengmeng , He Tianfeng , Wang Aihong , Lu Beibei , Liu Xiaobo , Xu Guozhang , Liu Qiyong
Published: November 2017

Few studies have been conducted to investigate the acute health effects of visibility and haze, which may be regarded as proxy indicators of ambient air pollution. We used a distributed lag non-linear model (DLNM) combined with quasi-Poisson regression to estimate the relationship between visibility, haze and mortality in Ningbo, a coastal city of China. We found that the mortality risk of visibility was statistically significant only on the current day, while the risk of haze and PM10 peaked on the second day and could last for three days. When the visibility was less than 10 km, each 1 km decrease of visibility at lag 0 day was associated with a 0.78% (95% CI: 0.22–1.36%) increase in total mortality and a 1.61% (95% CI: 0.39–2.85%) increase in respiratory mortality. The excess risk of haze at lag 0–2 days on total mortality, cardiovascular and respiratory mortality was 7.76% (95% CI: 3.29–12.42%), 7.73% (95% CI: 0.12–15.92%) and 17.77% (95% CI: 7.64–28.86%), respectively. Greater effects of air pollution were observed during the cold season than in the warm season, and the elderly were at higher risk compared to youths. The effects of visibility and haze were attenuated by single pollutants. These findings suggest that visibility and haze could be used as surrogates of air quality where pollutant data are scarce, and strengthen the evidence to develop policy to control air pollution and protect vulnerable populations.
Optimizing Cruising Routes for Taxi Drivers Using a Spatio-Temporal Trajectory Model
Authors: Wu Liang , Hu Sheng , Yin Li , Wang Yazhou , Chen Zhanlong , Guo Mingqiang , Chen Hao , Xie Zhong
Published: November 2017

Much of the taxi route-planning literature has focused on driver strategies for finding passengers and determining the hot spot pick-up locations using historical global positioning system (GPS) trajectories of taxis based on driver experience, distance from the passenger drop-off location to the next passenger pick-up location and the waiting times at recommended locations for the next passenger. The present work, however, considers the average taxi travel speed mined from historical taxi GPS trajectory data and the allocation of cruising routes to more than one taxi driver in a small-scale region to neighboring pick-up locations. A spatio-temporal trajectory model with load balancing allocations is presented to not only explore pick-up/drop-off information but also provide taxi drivers with cruising routes to the recommended pick-up locations. In simulation experiments, our study shows that taxi drivers using cruising routes recommended by our spatio-temporal trajectory model can significantly reduce the average waiting time and travel less distance to quickly find their next passengers, and the load balancing strategy significantly alleviates road loads. These objective measures can help us better understand spatio-temporal traffic patterns and guide taxi navigation.
Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
Authors: Sulheim Einar , Iversen Tore-Geir , To Nakstad Vu , Klinkenberg Geir , Sletta Håvard , Schmid Ruth , Hatletveit Anne , Wågbø Ane , Sundan Anders , Skotland Tore , Sandvig Kirsten , Mørch Ýrr
Published: November 2017

Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batches. Here we report the toxicity of poly(alkyl cyanoacrylate) nanoparticles in 12 different cell lines after synthesizing and analyzing 19 different nanoparticle batches and report that large variations were obtained when using different cell lines or various toxicity assays. Surprisingly, we found that nanoparticles with intermediate degradation rates were less toxic than particles that were degraded faster or more slowly in a cell-free system. The toxicity did not vary significantly with either the three different combinations of polyethylene glycol surfactants or with particle size (range 100–200 nm). No acute pro- or anti-inflammatory activity on cells in whole blood was observed.
Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain
Authors: Jo Yeong-Min , Cho Kyoungwon , Lee Hye-Jung , Lim Sun-Hyung , Kim Jin , Kim Young-Mi , Lee Jong-Yeol
Published: November 2017

Rice (Oryza sativa L.) is a primary global food cereal. However, when compared to wheat, rice has poor food processing qualities. Dough that is made from rice flour has low viscoelasticity because rice seed lacks storage proteins that are comparable to gluten protein from wheat. Thus, current research efforts aim to improve rice flour processing qualities through the transgenic expression of viscoelastic proteins in rice seeds. In this study, we characterized the transgenic expression of wheat glutenin subunits in rice seeds. The two genes 1Dx5_KK and 1Dy10_JK, which both encode wheat high-molecular-weight glutenin subunits that confer high dough elasticity, were cloned from Korean wheat cultivars KeumKang and JoKyung, respectively. These genes were inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter and were expressed in the high-amylose Korean rice cultivar Koami (Oryza sativa L.). Individual expression of both glutenin subunits was confirmed by SDS-PAGE and immunoblot analyses performed using T3 generation of transgenic rice seeds. The subcellular localization of 1Dx5_KK and 1Dy10_JK in the rice seed endosperm was confirmed by immunofluorescence analysis, indicating that the wheat glutenin subunits accumulate in protein body-II and novel protein body types in the rice seed. These results contribute to our understanding of engineered seed storage proteins in rice.
Illumination of a Vision—How Arthur Rimbaud Will Give Us Motivation to Find New Input into Bladder Cancer Biomarker Research
Authors: Ecke Thorsten , Otto Thomas
Published: November 2017

Bladder cancer (BC) accounts for approximately 430,000 new cases and 165,000 deaths each year worldwide [...]
Green Routes for the Production of Enantiopure Benzylisoquinoline Alkaloids
Authors: Ghirga Francesca , Bonamore Alessandra , Calisti Lorenzo , D’Acquarica Ilaria , Mori Mattia , Botta Bruno , Boffi Alberto , Macone Alberto
Published: November 2017

Benzylisoquinoline alkaloids (BIAs) are among the most important plant secondary metabolites, in that they include a number of biologically active substances widely employed as pharmaceuticals. Isolation of BIAs from their natural sources is an expensive and time-consuming procedure as they accumulate in very low levels in plant. Moreover, total synthesis is challenging due to the presence of stereogenic centers. In view of these considerations, green and scalable methods for BIA synthesis using fully enzymatic approaches are getting more and more attention. The aim of this paper is to review fully enzymatic strategies for producing the benzylisoquinoline central precursor, (S)-norcoclaurine and its derivatives. Specifically, we will detail the current status of synthesis of BIAs in microbial hosts as well as using isolated and recombinant enzymes.
Bevacizumab for Patients with Recurrent Multifocal Glioblastomas
Authors: Burger Michael , Breuer Stella , Cieplik Hans , Harter Patrick , Franz Kea , Bähr Oliver , Steinbach Joachim
Published: November 2017

In patients with glioblastoma, antiangiogenic therapy with bevacizumab (BEV) has been shown to improve progression-free survival (PFS), but not overall survival (OS). Especially in patients with an unusual infiltrative phenotype as seen in multifocal glioblastoma, the use of BEV therapy is still more controversial. Therefore, we prepared a retrospective case series with 16 patients suffering from a multifocal glioblastoma treated with BEV. We compared these patients to a matched control cohort of 16 patients suffering from glioblastoma with a single lesion treated with BEV. The objective of this study was to evaluate whether the course of disease differs in glioblastoma patients with a multifocal disease pattern compared to those with a single lesion only. Patients were treated with BEV monotherapy or BEV in combination with irinotecan or lomustine (CCNU). Response rates and PFS were similar in both groups. There was a trend for an unfavorable OS in the patient group with multifocal glioblastoma, which was expected due to the generally worse prognosis of multifocal glioblastoma. We investigated whether BEV therapy affects the invasive growth pattern as measured by the appearance of new lesions on magnetic resonance imaging (MRI). Under BEV therapy, there was a trend for a lower frequency of new lesions both in multifocal and solitary glioblastoma. Based on these results, BEV therapy at relapse appears to be justified to no lesser extent in multifocal glioblastoma than in solitary glioblastoma.
A Novel Mutation in the Fibrinogen Bβ Chain (c.490G>A; End of Exon 3) Causes a Splicing Abnormality and Ultimately Leads to Congenital Hypofibrinogenemia
Authors: Taira Chiaki , Matsuda Kazuyuki , Arai Shinpei , Sugano Mitsutoshi , Uehara Takeshi , Okumura Nobuo
Published: November 2017

We found a novel heterozygous mutation in the fibrinogen Bβ chain (c.490G>A) of a 3-year-old girl with congenital hypofibrinogenemia. To clarify the complex genetic mechanism, we made a mini-gene including a FGB c.490G>A mutation region, transfected it into a Chinese Hamster Ovary (CHO) cell line, and analyzed reverse transcription (RT) products. The assembly process and secretion were examined using recombinant mutant fibrinogen. Direct sequencing demonstrated that the mutant RT product was 99 bp longer than the wild-type product, and an extra 99 bases were derived from intron 3. In recombinant expression, a mutant Bβ-chain was weakly detected in the transfected CHO cell line, and aberrant fibrinogen was secreted into culture media; however, an aberrant Bβ-chain was not detected in plasma. Since the aberrant Bβ-chain was catabolized faster in cells, the aberrant Bβ-chain in a small amount of secreted fibrinogen may catabolize in the bloodstream. FGB c.490G>A indicated the activation of a cryptic splice site causing the insertion of 99 bp in intron 3. This splicing abnormality led to the production of a Bβ-chain possessing 33 aberrant amino acids, including two Cys residues in the coiled-coil domain. Therefore, a splicing abnormality may cause impaired fibrinogen assembly and secretion.
Advanced Glycation End Products in the Pathogenesis of Psoriasis
Authors: Papagrigoraki Anastasia , Maurelli Martina , Del Giglio Micol , Gisondi Paolo , Girolomoni Giampiero
Published: November 2017

Advanced glycation end products (AGEs) are extremely oxidant and biologically reactive compounds, which form through oxidation of sugars, lipids and amino acids to create aldehydes that bind covalently to proteins. AGEs formation and accumulation in human tissues is a physiological process during ageing but it is enhanced in case of persistent hyperglycemia, hyperlipidemia and oxidative or carbonyl stress, which are common in patients with moderate to severe psoriasis. Exogenous AGEs may derive from foods, UV irradiation and cigarette smoking. AGEs elicit biological functions by activating membrane receptors expressed on epithelial and inflammatory cell surface. AGEs amplify inflammatory response by favoring the release of cytokines and chemokines, the production of reactive oxygen species and the activation of metalloproteases. AGEs levels are increased in the skin and blood of patients with severe psoriasis independently of associated metabolic disorders. Intensified glycation of proteins in psoriasis skin might have a role in fueling cutaneous inflammation. In addition, AGEs released from psoriatic skin may increase metabolic and cardiovascular risk in patients with severe disease.
A Single-Extremity Staged Approach for Critical Congenital Heart Disease Screening: Results from Tennessee
Authors: Walsh William , Ballweg Jean
Published: November 2017

Tennessee initiated single-extremity staged screening by pulse oximetry for undetected CCHD in 2012. The algorithm begins with a saturation reading in the foot and allows an automatic pass if the foot pulse oximetry is 97% or greater. This was based on the principle that it is not possible to have a greater than 4% difference in the pulse oximetry between upper and lower extremities if the lower extremity is equal to or greater than 97%. This approach eliminates over 75,000 “unnecessary” pulse oximetry determinations in Tennessee each year without affecting the ability to detect CCHD before hospital discharge.
Kinking and Torsion Can Significantly Improve the Efficiency of Valveless Pumping in Periodically Compressed Tubular Conduits. Implications for Understanding of the Form-Function Relationship of Embryonic Heart Tubes
Authors: Hiermeier Florian , Männer Jörg
Published: November 2017

Valveless pumping phenomena (peristalsis, Liebau-effect) can generate unidirectional fluid flow in periodically compressed tubular conduits. Early embryonic hearts are tubular conduits acting as valveless pumps. It is unclear whether such hearts work as peristaltic or Liebau-effect pumps. During the initial phase of its pumping activity, the originally straight embryonic heart is subjected to deforming forces that produce bending, twisting, kinking, and coiling. This deformation process is called cardiac looping. Its function is traditionally seen as generating a configuration needed for establishment of correct alignments of pulmonary and systemic flow pathways in the mature heart of lung-breathing vertebrates. This idea conflicts with the fact that cardiac looping occurs in all vertebrates, including gill-breathing fishes. We speculate that looping morphogenesis may improve the efficiency of valveless pumping. To test the physical plausibility of this hypothesis, we analyzed the pumping performance of a Liebau-effect pump in straight and looped (kinked) configurations. Compared to the straight configuration, the looped configuration significantly improved the pumping performance of our pump. This shows that looping can improve the efficiency of valveless pumping driven by the Liebau-effect. Further studies are needed to clarify whether this finding may have implications for understanding of the form-function relationship of embryonic hearts.
Function and Biosynthesis of Cell Wall α-1,3-Glucan in Fungi
Authors: Yoshimi Akira , Miyazawa Ken , Abe Keietsu
Published: November 2017

Although α-1,3-glucan is a major cell wall polysaccharide in filamentous fungi, its biological functions remain unclear, except that it acts as a virulence factor in animal and plant pathogenic fungi: it conceals cell wall β-glucan on the fungal cell surface to circumvent recognition by hosts. However, cell wall α-1,3-glucan is also present in many of non-pathogenic fungi. Recently, the universal function of α-1,3-glucan as an aggregation factor has been demonstrated. Applications of fungi with modified cell wall α-1,3-glucan in the fermentation industry and of in vitro enzymatically-synthesized α-1,3-glucan in bio-plastics have been developed. This review focuses on the recent progress in our understanding of the biological functions and biosynthetic mechanism of cell wall α-1,3-glucan in fungi. We briefly consider the history of studies on α-1,3-glucan, overview its biological functions and biosynthesis, and finally consider the industrial applications of fungi deficient in α-1,3-glucan.
(±)-Methyl 1,1a,2,7b-Tetrahydro-2-oxocyclopropa[c] Chromene-1a-carboxylate
Authors: Choi Sunyoung , Kim Sung-Gon
Published: November 2017

A novel method for the preparation of (±)-methyl 1,1a,2,7b-tetrahydro-2-oxocyclopropa[c]chromene-1a-carboxylate has been developed. The cyclopropanation of methyl coumarin-3-carboxylate provided title compound in moderate yield by dropwise treatment with 1.5 equiv. of dimethylsulfoxonium methylide over 6 h. The structure of the newly synthesized compound was determined using 1H-, 13C-NMR, IR and mass spectral data.
Efficiency of True-Green Light Emitting Diodes: Non-Uniformity and Temperature Effects
Authors: Titkov Ilya , Karpov Sergey , Yadav Amit , Mamedov Denis , Zerova Vera , Rafailov Edik
Published: November 2017

External quantum efficiency of industrial-grade green InGaN light-emitting diodes (LEDs) has been measured in a wide range of operating currents at various temperatures from 13 K to 300 K. Unlike blue LEDs, the efficiency as a function of current is found to have a multi-peak character, which could not be fitted by a simple ABC-model. This observation correlated with splitting of LED emission spectra into two peaks at certain currents. The characterization data are interpreted in terms of non-uniformity of the LED active region, which is tentatively attributed to extended defects like V-pits. We suggest a new approach to evaluation of temperature-dependent light extraction and internal quantum efficiencies taking into account the active region non-uniformity. As a result, the temperature dependence of light extraction and internal quantum efficiencies have been evaluated in the temperature range mentioned above and compared with those of blue LEDs.
Facile Control of the Porous Structure of Larch-Derived Mesoporous Carbons via Self-Assembly for Supercapacitors
Authors: Zhao Xin , Li Wei , Chen Honglei , Wang Shoujuan , Kong Fangong , Liu Shouxin
Published: November 2017

Mesoporous carbons have been successfully synthesized via self-assembly using larch-based resins as precursors and triblock copolymers as soft templates. The porous structure of mesoporous carbons can be tailored by adjusting the ratio of hydrophilic/hydrophobic (EO/PO) units owing to interfacial curvature. Interestingly, the porous structures show a distinct change from vortex-like to worm-like pores, to stripe-like pores, and to ordered two-dimensional hexagonal pores as the ratio of hydrophilic/hydrophobic units increases, indicating the significant effect of EO/PO ratio on the porous structure. The mesoporous carbons as supercapacitor electrodes exhibit superior electrochemical capacitive performance and a high degree of reversibility after 2000 cycles for supercapacitors due to the well-defined mesoporosity of the carbon materials. Meanwhile, the superior carbon has a high specific capacitance of 107 F·g−1 in 6 M KOH at a current density of 10 A·g−1.
Generalized Langevin Equation and the Prabhakar Derivative
Authors: Sandev Trifce
Published: November 2017

We consider a generalized Langevin equation with regularized Prabhakar derivative operator. We analyze the mean square displacement, time-dependent diffusion coefficient and velocity autocorrelation function. We further introduce the so-called tempered regularized Prabhakar derivative and analyze the corresponding generalized Langevin equation with friction term represented through the tempered derivative. Various diffusive behaviors are observed. We show the importance of the three parameter Mittag-Leffler function in the description of anomalous diffusion in complex media. We also give analytical results related to the generalized Langevin equation for a harmonic oscillator with generalized friction. The normalized displacement correlation function shows different behaviors, such as monotonic and non-monotonic decay without zero-crossings, oscillation-like behavior without zero-crossings, critical behavior, and oscillation-like behavior with zero-crossings. These various behaviors appear due to the friction of the complex environment represented by the Mittag-Leffler and tempered Mittag-Leffler memory kernels. Depending on the values of the friction parameters in the system, either diffusion or oscillations dominate.
Multilevel Analysis of Employee Satisfaction on Commitment to Organizational Culture: Case Study of Chinese State-Owned Enterprises
Authors: Liu Fangtao , Tso Kwok , Yang Yongheng , Guan Jingjing
Published: November 2017

This study analyzes the effects of employee satisfaction and demographic indicators on employee commitment to organizational culture at the enterprise level. With data from a survey of 3029 employees from 27 state-owned enterprises (SOEs), a hierarchical linear model (HLM) is used to identify the influencing factors of employee commitment to organizational culture at the enterprise level. An empirical study indicates that apart from the factors of employee satisfaction and demographic background, four contextual variables of enterprises, namely, comprehensive management, energy intensity, cost-income ratio, and capacity-load ratio, also influence commitment to organizational culture levels. Results show that applying HLM can substantially improve the explanatory power of employee satisfaction factors on commitment to organizational culture using nested enterprise contextual variables. Although measurement scales and satisfaction models have been proposed over the years, only a few studies have addressed the particular nature inherent in Chinese SOEs. HLM, which accounts for the nested data structure and determines the effects of employee satisfaction factors on commitment to organizational culture without bias, is developed in this study. Through an insider view based on empirical work, this research can improve the ability of senior managers to understand the culture and dynamics of organizations, to deliver strong leadership, and to enhance corporate internal management.
Endogenous Sphingolipid Signaling Pathway Implicated in the Action of Croton membranaceus on the Prostate Gland in BPH Patients
Authors: Asare George , Anang Yvonne , Afriyie Daniel , Amoah Brodrick , Asiedu Bernice , Doku Derek , Ocansey Hannah , Odei Danso Nana , Tekpor Prince , Osam Sarah
Published: November 2017

Background: Croton membranaceus extract has apoptotic effects on BPH-1 cells. This study determined if the apoptotic effects were created through the ceramide pathway. Methods: The study was a follow-up to a previous observational study of 30 histologically confirmed patients with benign prostatic hyperplasia (BPH) who were on C. membranaceus ethanolic extract at 20 mg t.i.d orally for 3 mo. Thereafter, total and free prostate-specific antigen (PSA), lipid profile plus Apo lipoprotein A and B, ceramide/Sphingophospho-kinase 1 (SphK1) and 2 (SphK2), sphingosine lyase (SPL), the cytotoxic adducts of oxidative stress 4-hydroxy-2-nonenal (4HNE) and malondialdehyde (MDA), were determined. Results: Total and free PSA were significantly (p < 0.05) different after treatment. Apo lipoprotein A was significantly different (p = 0.024). The SphK1/SphK2 ratio reduced significantly (p = 0.049). Furthermore, SPL, ceramide, and MDA increased significantly after treatment (p = 0.05, p = 0.004, and p = 0.007, respectively). A weak positive correlation was found between high-density lipoprotein (HDL) cholesterol and SphK1, and HDL and ceramide before treatment (p = 0.036, r = 0.3826; p = 0.018, r = 0.4286, respectively. Conclusions: C. membranaceus uses the ceramide pathway by modulating the SphK1/SphK2 ratio and increasing SPL to generate oxidative stress and consequently apoptosis.
Assessment of the Contour Method for 2-D Cross Sectional Residual Stress Measurements of Friction Stir Welded Parts of AA2024-T3—Numerical and Experimental Comparison
Authors: Sonne Mads , Carlone Pierpaolo , Hattel Jesper
Published: November 2017

The contour method is one of the newest techniques for obtaining residual stress fields from friction stir welded (FSW) parts, experimentally. This method has many advantages; however, edge effects coming from the process itself might introduce artifacts in the obtained results, and this was slightly touched upon in the very first paper on the method. This concern is further assessed in the present work, where the contour method is compared with the results that were obtained numerically via a thermomechanical model and experimentally via the cut-compliance method. For the two-dimensional (2-D) cross sectional map obtained by the method, peak stresses in tension are observed in the mid-section of the FSW butt-welded plates at the distance of the tool radius from the centerline. The corresponding numerical simulation indicates the same behavior because of the particular clamping conditions, and consequently this should not be interpreted as a misleading result of the contour method. Edge effects from the cutting process involved in the contour method should, however, be taken into consideration, most likely resulting in the residual stresses observed near the surfaces of the cross section being less extreme in reality than observed.
Entropy Change Caused by Martensitic Transformations of Ferromagnetic Shape Memory Alloys
Authors: L’vov Victor , Cesari Eduard , Kosogor Anna , Torrens-Serra Joan , Recarte Vicente , Pérez-Landazábal José
Published: November 2017

In this paper, our most recent findings on the influence of magnetic order on the main transformational caloric and elastic properties of shape memory alloys (SMAs) are reviewed. It is argued that ferromagnetic order has a strong influence on the temperature interval of martensitic transformation (MT), the characteristics of stress-induced MT, and the shear elastic modulus of SMA. The problem of separation of the magnetic contributions to the entropy change ΔS and heat Q exchanged in the course of martensitic transformation (MT) of SMA is considered in general terms, and theoretical formulas enabling the solution of the problem are presented. As an example, the ΔS and Q values, which were experimentally determined for Ni-Mn-Ga and Ni-Fe-Ga alloys with different Curie temperatures TC and MT temperatures TM, are theoretically analyzed. It is shown that for Ni-Mn-Ga martensites with TM < TC, the ratio of elastic and magnetic contributions to the entropy change may be greater or smaller than unity, depending on the temperature difference TC – TM.
Computational Approaches for Integrative Analysis of the Metabolome and Microbiome
Authors: Chong Jasmine , Xia Jianguo
Published: November 2017

The study of the microbiome, the totality of all microbes inhabiting the host or an environmental niche, has experienced exponential growth over the past few years. The microbiome contributes functional genes and metabolites, and is an important factor for maintaining health. In this context, metabolomics is increasingly applied to complement sequencing-based approaches (marker genes or shotgun metagenomics) to enable resolution of microbiome-conferred functionalities associated with health. However, analyzing the resulting multi-omics data remains a significant challenge in current microbiome studies. In this review, we provide an overview of different computational approaches that have been used in recent years for integrative analysis of metabolome and microbiome data, ranging from statistical correlation analysis to metabolic network-based modeling approaches. Throughout the process, we strive to present a unified conceptual framework for multi-omics integration and interpretation, as well as point out potential future directions.
Light-Activated Metal Oxide Gas Sensors: A Review
Authors: Xu Fang , HO Ho-Pui
Published: November 2017

Conductometric gas sensors facilitated by photons have been investigated for decades. Light illumination may enhance device attributes including operational temperature, sensing sensitivity and selectivity. This paper aims to provide an overview on the progress of light-activated gas sensors, with a specific focus on sensors based on metal oxides. The material systems that have been studied include pure metal oxides, heterostructures of semiconductor-metal oxides and metal-metal oxides, and metal oxides with dopant. Other reported works on the use of different nanostructures such as one-dimensional and porous nanostructures, study of sensing mechanisms and the interplay between various factors are also summarized. Possible directions for further improvement of sensing properties, through optimizing the size of nanomaterials, film thickness, light intensity and wavelength are discussed. Finally, we point out that the main challenge faced by light-activated gas sensors is their low optical response, and we have analyzed the feasibility of using localized surface plasmon resonance to solve this drawback. This article should offer readers some key and instructive insights into the current and future development of light-activated gas sensors.
Fabrication and Actuation of an Electrowetting Droplet Array on a Flexible Substrate
Authors: Van Grinsven Kari , Ousati Ashtiani Alireza , Jiang Hongrui
Published: November 2017

Electrowetting-on-dielectric (EWOD) is a fast, well-established actuation method for a variety of applications, from microfluidics to electrowetting displays to electrowetting lenses. We therefore seek to develop a robust, scalable fabrication method for the realization of EWOD on a flexible polydimethylsiloxane (PDMS) substrate in order to increase the range of possible applications. We fabricated a 5 × 5 array of individually controlled electrowetting cells to manipulate silicone oil droplets via EWOD. The fabrication process utilized exclusively flexible materials to improve the robustness of the overall device, and processing methods were adapted to accommodate the particular challenges posed by flexible materials. Simulation of the EWOD devices was conducted using ANSYS Fluent and showed the change in contact angle in response to voltage applied. Fabricated devices were also tested, with actuation of the oil droplet observed with up to 100 V (RMS) AC applied across underlying electrodes. We demonstrated fabrication of a fully flexible array and verified actuation to center droplets over the electrodes. This work may be expanded to address more specific flexible applications for EWOD.
A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle
Authors: Aziz Aziz , Fu Mengjie , Deng Jiu , Geng Chunyang , Luo Yong , Lin Bingcheng , Yu Xiaohui , Liu Bo
Published: November 2017

Microfluidic chips have been proved effective in mimicking different organs of human body. Simulating human ovarian follicles by microfluidic device will be useful in exploring the mechanism of folliculogenesis and related diseases. In this paper, a microfluidic chip was designed to culture a single human pre-antral follicle. Ovarian follicles were first encapsulated in 3D calcium alginate hydrogel beads and then cultured on chip and in dish under same conditions. The diameters of cultured ovarian follicles were measured, and the same amount of medium was collected from microfluidic device or dish per two days for measuring the estradiol and androgen concentrations. The results confirmed the successful growth of ovarian follicles on chip with their hormonal trends and diameters increase, which were similar to ovarian follicles cultured in dish. It is concluded that this microfluidic chip can be used to culture a single human ovarian follicle, which provides a useful tool to explore the hormonal changes and their interactions during folliculogenesis.
Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme
Authors: Dassa Bareket , Borovok Ilya , Lombard Vincent , Henrissat Bernard , Lamed Raphael , Bayer Edward A. , Moraïs Sarah
Published: November 2017

The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides. Several cellulolytic bacteria have evolved various elaborate modular architectures of active cellulosomes. We present here a genome-wide analysis of a dozen mesophilic clostridia species, including both well-studied and yet-undescribed cellulosome-producing bacteria. We first report here, the presence of cellulosomal elements, thus expanding our knowledge regarding the prevalence of the cellulosomal paradigm in nature. We explored the genomic organization of key cellulosome components by comparing the cellulosomal gene clusters in each bacterial species, and the conserved sequence features of the specific cellulosomal modules (cohesins and dockerins), on the background of their phylogenetic relationship. Additionally, we performed comparative analyses of the species-specific repertoire of carbohydrate-degrading enzymes for each of the clostridial species, and classified each cellulosomal enzyme into a specific CAZy family, thus indicating their putative enzymatic activity (e.g., cellulases, hemicellulases, and pectinases). Our work provides, for this large group of bacteria, a broad overview of the blueprints of their multi-component cellulosomal complexes. The high similarity of their scaffoldin clusters and dockerin-based recognition residues suggests a common ancestor, and/or extensive horizontal gene transfer, and potential cross-species recognition. In addition, the sporadic spatial organization of the numerous dockerin-containing genes in several of the genomes, suggests the importance of the cellulosome paradigm in the given bacterial species. The information gained in this work may be utilized directly or developed further by genetically engineering and optimizing designer cellulosome systems for enhanced biotechnological biomass deconstruction and biofuel production.