bluuebunny/crossref_metadata_2025 huggingface dataset:
Polyneuropathy associated with chronic hemodialysis: Clinical and electrophysiological study
Authors: Ezzeldin Nillie , Abdel Galil Sahar Mahfouz , Said Dina , Kamal Nafesa Mohamed , Amer Mona
Published: December 2018

Aim

To estimate the frequency and pattern of peripheral polyneuropathy (PNP) that may affect patients maintained on hemodialysis.

Patients and methods

The study was carried out on 60 middle‐aged male patients attending the Internal Medicine Department for maintenance hemodialysis. All were subjected to a complete neurological examination. Motor and sensory nerve conduction studies of both lower limbs (the tibial, peroneal and sural nerves) and both upper limbs (median and ulnar nerves), as well as F‐wave measurements of both tibial and median nerves, were done. The patients were subdivided clinically into two groups, clinically apparent neuropathy and inapparent groups. Then they were divided according to the types of peripheral neuropathy detected by electrophysiological studies into axonal, demyelinated and mixed polyneuropathy. In addition, they were divided into motor, sensory and sensorimotor groups.

Results

Polyneuropathy was found clinically presented in 33 (55%) cases, while evident by electrophysiological examination in 100% of the clinically apparent group (33 patients) and evident in 92.5% of the clinically inapparent group (27 patients). The frequency of pathologic electrophysiological parameters was significantly higher in patients with longer duration of hemodialysis. Axonal polyneuropathy is the most prevalent type in those patients.

Conclusion

Peripheral polyneuropathy is a common presentation in patients maintained on hemodialysis. The longer the duration of hemodialysis, the more liability to develop PNP that can be detected earlier by electrodiagnostic studies in the subclinical cases.
House dust mite drives proinflammatory eicosanoid reprogramming and macrophage effector functions
Authors: Henkel Fiona D. R. , Friedl Antonie , Haid Mark , Thomas Dominique , Bouchery Tiffany , Haimerl Pascal , de los Reyes Jiménez Marta , Alessandrini Francesca , Schmidt‐Weber Carsten B. , Harris Nicola L. , Adamski Jerzy , Esser‐von Bieren Julia
Published: December 2018

Background

Eicosanoid lipid mediators play key roles in type 2 immune responses, for example in allergy and asthma. Macrophages represent major producers of eicosanoids and they are key effector cells of type 2 immunity. We aimed to comprehensively track eicosanoid profiles during type 2 immune responses to house dust mite (HDM) or helminth infection and to identify mechanisms and functions of eicosanoid reprogramming in human macrophages.

Methods

We established an LC‐MS/MS workflow for the quantification of 52 oxylipins to analyze mediator profiles in human monocyte‐derived macrophages (MDM) stimulated with HDM and during allergic airway inflammation (AAI) or nematode infection in mice. Expression of eicosanoid enzymes was studied by qPCR and western blot and cytokine production was assessed by multiplex assays.

Results

Short (24 h) exposure of alveolar‐like MDM (aMDM) to HDM suppressed 5‐LOX expression and product formation, while triggering prostanoid (thromboxane and prostaglandin D2 and E2) production. This eicosanoid reprogramming was p38‐dependent, but dectin‐2‐independent. HDM also induced proinflammatory cytokine production, but reduced granulocyte recruitment by aMDM. In contrast, high levels of cysteinyl leukotrienes (cysLTs) and 12‐/15‐LOX metabolites were produced in the airways during AAI or nematode infection in mice.

Conclusion

Our findings show that a short exposure to allergens as well as ongoing type 2 immune responses are characterized by a fundamental reprogramming of the lipid mediator metabolism with macrophages representing particularly plastic responder cells. Targeting mediator reprogramming in airway macrophages may represent a viable approach to prevent pathogenic lipid mediator profiles in allergy or asthma.
Genetics of ncHH: from a peculiar inheritance of a novel GNRHR mutation to a comprehensive review of the literature
Authors: Cioppi F. , Riera‐Escamilla A. , Manilall A. , Guarducci E. , Todisco T. , Corona G. , Colombo F. , Bonomi M. , Flanagan C. A. , Krausz C.
Published: December 2018

Background

Normosmic congenital hypogonadotropic hypogonadism (ncHH) is caused by the deficient production, secretion, or action of gonadotropin‐releasing hormone (GnRH). Its typical clinical manifestation is delayed puberty and azoospermia. Homozygous and compound heterozygous mutations in the GNRHR gene (4q13.2) are the most frequent genetic causes of ncHH.

Objectives

(i) Characterization at the molecular level (genetic origin and functional effect) of a unique homozygous mutation (p.Gly99Glu) in a ncHH man; (ii) to provide a comprehensive catalog of GNRHR mutations with genotype–phenotype correlation and comparison of in vitro studies vs. in silico prediction tools.

Material and Methods

A ncHH man and his parents, in whom we performed the following: (i) Sanger sequencing, qPCR of the GNRHR gene; (ii) chromosome 4 SNP array; and (iii) competition binding assay and inositol phosphate signaling assay. PubMed and Human Genome Mutation Database (HGMD) search for GNRHR mutations. Bioinformatic analysis of 55 reported variants.

Results

qPCR showed two GNRHR copies in the index case. SNP array revealed the inheritance of two homologous chromosomes 4 from the mother (maternal heterodisomy; hUPD) with two loss of heterozygosity regions, one of them containing the mutated gene (maternal isodisomy; iUPD). Functional studies for the p.Gly99Glu mutation demonstrated a right‐shifted GnRH‐stimulated signaling response. Bioinformatic tools show that commonly used in silico tools are poor predictors of the function of ncHH‐associated GNRHR variants.

Discussion

Functional analysis of the p.Gly99Glu mutation is consistent with severely decreased GnRH binding affinity (a severe partial loss‐of‐function mutation). Complete LOF variants are associated with severe and severe/moderate phenotype, whereas partial LOF variants show wide range of clinical manifestations.

Conclusion

This is the first ncHH patient carrying a novel causative missense mutation of GNRHR with proven ‘severe pLOF’ due to maternal hUPD/iUPD of chromosome 4. Our literature review shows that functional studies remain essential both for diagnostic and potential therapeutic purposes.
Fiction in Fact: Ernest Callenbach’sEcotopiaand the Creation of a Green Culture with Anthropological Ingredients
Authors: Lohmann Roger Ivar
Published: December 2018

Ecotopia is a quasi‐ethnographic novel influenced by anthropology. Some of its fictional practices have been adopted in the actual world since publication in 1975. Set two decades after Green secessionists achieve national independence for the U.S. Northwest, an American journalist‐fieldworker describes Ecotopians’ strange new ecological customs. Author Callenbach constructively criticized and inflenced his unsustainable culture by comparing it to an invented alternative. The case illustrates that a dialectic between cultural fact and fiction drives innovation.

“So a light went on in my head: if a country doesn’t exist that gets its bleep together, maybe it’s time to start thinking about inventing one.”

—Ernest Callenbach (Smith 2010)
Effect of wood kraft pulp feed on digestibility, ruminal characteristics, and milk production performance in lactating dairy cows
Authors: Nishimura Keiko , Kurosu Kazuhiro , Terada Fuminori , Mizuguchi Hitoshi , Sato Shigeru , Kushibiki Shiro
Published: December 2018

The effect of wood kraft pulp (KP) feed on dietary digestibility, ruminal fluid pH, rumen fermentation characteristics, and milk production performance in lactating dairy cows was examined. Four lactating dairy cows were used for the feeding experiment by the cross‐over design. The control group and KP group were set up as treatments. The control group was fed total mixed ration (TMR) (40% roughage and 60% concentrate) and the KP group was fed TMR containing 12% KP that replaced half of the rolled corn in the control diet. The dry matter intake, digestibility of the feed components, and milk yield were not significantly different between control group and KP group. The number of times that the ruminal fluid pH was below 6.1 tended to decrease in the KP group compared to the control group (p < 0.10). The acetic acid ratio in the ruminal fluid of the KP group increased compared to the control group (p < 0.05) and the propionic acid ratio in the ruminal fluid of the KP group decreased compared to the control group (p < 0.05). The acetate:propionate acid ratio was increased in the KP group compared with the control group (p < 0.05). Lipopolysaccharide levels in the ruminal fluid of the KP group tended to decrease compared to the control group (p < 0.10). Based on these results, it was indicated that the use of KP feed for lactating dairy cows induced the same rumen fermentation characteristics as those in cows given a large amount of roughage without depressing milk productivity. Therefore, KP could be a valuable feed resource substitute for grains, which would also reduce the risk for subacute rumen acidosis.
Atrial fibrillation and human immunodeficiency virus type‐1 infection: a systematic review. Implications for anticoagulant and antiarrhythmic therapy
Authors: Pastori Daniele , Mezzaroma Ivano , Pignatelli Pasquale , Violi Francesco , Lip Gregory Y. H.
Published: December 2018

The prevalence and incidence of atrial fibrillation/flutter (AF/AFL) in patients with human immunodeficiency virus type‐1 (HIV‐1) infection have been poorly investigated. We performed a systematic review using PubMed and Cochrane Database of Systematic Reviews, and screening of references, searching for clinical studies reporting on the association between HIV‐1 infection and AF/AFL. We also summarized the main interactions of antiretroviral agents with antithrombotic and antiarrhythmic drugs. We found a prevalence of AF/AFL ranging from 2.0% to 5.13% in patients with HIV‐1, with an incidence rate of 3.6/1000 person‐years. Low CD4+ count (<200–250 cells ml−1) and high viral load were predictors of AF/AFL. Regarding drugs interactions, nucleoside reverse transcriptase inhibitors, integrase inhibitor and maraviroc have the lowest interactions with oral anticoagulants. Among anticoagulants, dabigatran presents the most favourable profile. Most of antiarrhythmic drugs interact with protease inhibitors, with beta blockers and diltiazem having fewer interactions. The few studies available suggest a non‐negligible prevalence of AF/AFL in patients with HIV‐1 infection. Awareness of potential interactions with anticoagulation and antiarrhythmic drugs is needed to offer optimal management in this population.
Paleogene evolution and demise of the proto‐Paratethys Sea in Central Asia (Tarim and Tajik basins): Role of intensified tectonic activity at ca. 41 Ma
Authors: Kaya Mustafa Y. , Dupont‐Nivet Guillaume , Proust Jean‐Noël , Roperch Pierrick , Bougeois Laurie , Meijer Niels , Frieling Joost , Fioroni Chiara , Özkan Altıner Sevinç , Vardar Ezgi , Barbolini Natasha , Stoica Marius , Aminov Jovid , Mamtimin Mehmut , Zhaojie Guo
Published: December 2018

The proto‐Paratethys Sea covered a vast area extending from the Mediterranean Tethys to the Tarim Basin in western China during Cretaceous and early Paleogene. Climate modelling and proxy studies suggest that Asian aridification has been governed by westerly moisture modulated by fluctuations of the proto‐Paratethys Sea. Transgressive and regressive episodes of the proto‐Paratethys Sea have been previously recognized but their timing, extent and depositional environments remain poorly constrained. This hampers understanding of their driving mechanisms (tectonic and/or eustatic) and their contribution to Asian aridification. Here, we present a new chronostratigraphic framework based on biostratigraphy and magnetostratigraphy as well as a detailed palaeoenvironmental analysis for the Paleogene proto‐Paratethys Sea incursions in the Tajik and Tarim basins. This enables us to identify the major drivers of marine fluctuations and their potential consequences on Asian aridification. A major regional restriction event, marked by the exceptionally thick (≤ 400 m) shelf evaporites is assigned a Danian‐Selandian age (ca. 63–59 Ma) in the Aertashi Formation. This is followed by the largest recorded proto‐Paratethys Sea incursion with a transgression estimated as early Thanetian (ca. 59–57 Ma) and a regression within the Ypresian (ca. 53–52 Ma), both within the Qimugen Formation. The transgression of the next incursion in the Kalatar and Wulagen formations is now constrained as early Lutetian (ca. 47–46 Ma), whereas its regression in the Bashibulake Formation is constrained as late Lutetian (ca. 41 Ma) and is associated with a drastic increase in both tectonic subsidence and basin infilling. The age of the final and least pronounced sea incursion restricted to the westernmost margin of the Tarim Basin is assigned as Bartonian–Priabonian (ca. 39.7–36.7 Ma). We interpret the long‐term westward retreat of the proto‐Paratethys Sea starting at ca. 41 Ma to be associated with far‐field tectonic effects of the Indo‐Asia collision and Pamir/Tibetan plateau uplift. Short‐term eustatic sea level transgressions are superimposed on this long‐term regression and seem coeval with the transgression events in the other northern Peri‐Tethyan sedimentary provinces for the 1st and 2nd sea incursions. However, the 3rd sea incursion is interpreted as related to tectonism. The transgressive and regressive intervals of the proto‐Paratethys Sea correlate well with the reported humid and arid phases, respectively in the Qaidam and Xining basins, thus demonstrating the role of the proto‐Paratethys Sea as an important moisture source for the Asian interior and its regression as a contributor to Asian aridification.
Fluid flow and pore pressure development throughout the evolution of a trough mouth fan, western Barents Sea
Authors: Llopart Jaume , Urgeles Roger , Forsberg Carl Fredrik , Camerlenghi Angelo , Vanneste Maarten , Rebesco Michele , Lucchi Renata Giulia , Rüther Denise Christina , Lantzsch Hendrik
Published: December 2018

Using a combination of geophysical and geotechnical data from Storfjorden Trough Mouth Fan off southern Svalbard, we investigate the hydrogeology of the continental margin and how this is affected by Quaternary glacial advances and retreats over the continental shelf. The geotechnical results show that plumites, deposited during the deglaciation, have high porosities, permeabilities and compressibilities with respect to glacigenic debris flows and tills. These results together with margin stratigraphic models obtained from seismic reflection data were used as input for numerical finite element models to understand focusing of interstitial fluids on glaciated continental margins. The modelled evolution of the Storfjorden TMF shows that tills formed on the shelf following the onset of glacial sedimentation (ca. 1.5 Ma) acted as aquitards and therefore played a significant role in decreasing the vertical fluid flow towards the sea floor and diverting it towards the slope. The model shows that high overpressure ratios (up to λ ca. 0.6) developed below the shelf edge and on the middle slope. A more detailed model for the last 220 kyrs accounting for ice loading during glacial maxima shows that the formation of these aquitards on the shelf focused fluid flow towards the most permeable plumite sediments on the slope. The less permeable glacigenic debris flows that were deposited during glacial maxima on the slope hinder fluid evacuation from plumites allowing high overpressure ratios (up to λ ca. 0.7) to develop in the shallowest plumite layers. These high overpressures likely persist to the Present and are a critical precondition for submarine slope failure.
Dormancy in cancer
Authors: Endo Hiroko , Inoue Masahiro
Published: December 2018

The idea of tumor dormancy originated from clinical findings that recurrence of cancer occurs several years or even several decades after surgical resection of the primary tumor. Tumor mass dormancy was proposed as a model, where there is equal balance between increases in the number of cancer cells by proliferation and decreases as a result of cell death. Tumor mass dormancy includes angiogenic dormancy and immune‐mediated dormancy. Another emerging type of tumor dormancy is cellular dormancy in which cancer cells are in a quiescent state. Cellular dormancy is induced by cues such as the extracellular matrix environment, metastatic niches, a hypoxic microenvironment, and endoplasmic reticulum stress. Even the oncogenic pathways, on which active cancer cells depend for survival and growth, are suppressed in the dormant state. As tumor dormancy is one of the mechanisms of resistance against various cancer therapies, targeting dormant cancer cells should be considered for future treatment strategies.
Red flags for appropriate referral to the gastroenterologist and the rheumatologist of patients with inflammatory bowel disease and spondyloarthritis
Authors: Felice C , Leccese P , Scudeller L , Lubrano E , Cantini F , Castiglione F , Gionchetti P , Orlando A , Salvarani C , Scarpa R , Vecchi M , Olivieri I , Armuzzi A
Published: December 2018

Collaboration between gastroenterologists and rheumatologists is recommended for the correct management of patients with associated spondyloarthritis (SpA) and inflammatory bowel disease (IBD). We aimed to establish the appropriateness of several red flags for a prompt specialist referral. A systematic review of the literature was performed using the GRADE method to describe the prevalence of co-existing IBD-SpA and the diagnostic accuracy of red flags proposed by a steering committee. Then, a consensus among expert gastroenterologists and rheumatologists (10 in the steering committee and 13 in the expert panel) was obtained using the RAND method to confirm the appropriateness of each red flag as ‘major’ (one sufficient for patient referral) or ‘minor’ (at least three needed for patient referral) criteria for specialist referral. The review of the literature confirmed the high prevalence of co-existing IBD-SpA. Positive and negative predictive values of red flags were not calculated, given the lack of available data. A consensus among gastroenterology and rheumatology specialists was used to confirm the appropriateness of each red flag. Major criteria to refer patients with SpA to the gastroenterologist included: rectal bleeding, chronic abdominal pain, perianal fistula or abscess, chronic diarrhoea and nocturnal symptoms. Major criteria to refer patients with IBD to the rheumatologist included: chronic low back pain, dactylitis, enthesitis and pain/swelling of peripheral joints. Several major and minor red flags have been identified for the diagnosis of co-existing IBD-SpA. The use of red flags in routine clinical practice may avoid diagnostic delay and reduce clinic overload.
Thigh and abdominal adipose tissue depot associations with testosterone levels in postmenopausal females
Authors: Ofori Emmanuel K. , Conde Alonso Sonia , Correas‐Gomez Lorena , Carnero Elvis A. , Zwygart Karin , Hugues Henry , Bardy Daniel , Hans Didier , Dwyer Andrew A. , Amati Francesca
Published: December 2018

Objective

Research findings on the relationship between serum androgens and adipose tissue in older females are inconsistent. We aimed to clarify the relationship using state‐of‐the‐art techniques to evaluate associations between body fat distribution and plasma testosterone (T) levels in older postmenopausal women.

Design

Observational, cross‐sectional study of healthy, community dwelling postmenopausal women.

Patients and Measurements

Postmenopausal women (60‐80 years old) were included in this study. Overall body composition was evaluated by dual‐energy X‐ray absorptiometry. Abdominal and thigh fat depots were measured by magnetic resonance imaging. Circulating T concentrations were analysed by liquid chromatography‐tandem mass spectrometry.

Results

Thirty‐five women (66.6 ± 0.8 years) participated in this study. T levels were positively associated with clinical proxy measures of adiposity including weight (ρ = 0.39), BMI (ρ = 0.43) and waist circumference (ρ = 0.39) (all P < 0.05). Fat mass and % body fat were correlated with T levels (ρ = 0.42 and 0.38 respectively, both P < 0.05). T correlated with overall and superficial abdominal fat (ρ = 0.34 and 0.37 respectively, both P < 0.05) but not with visceral adipose tissue. T increased with greater thigh fat (ρ = 0.49, P < 0.05) in both superficial and deep depots (ρ = 0.50 and 0.35 respectively, both P < 0.05).

Conclusion

Our results suggest that postmenopausal women with higher circulating T levels have both higher regional and overall body adiposity. These findings underscore the sexual dimorphism in the relationship between serum androgen levels and adiposity.
Enigma portal case: Phaeohyphomycosis by fine needle aspiration
Authors: Jug Rachel , Shattuck Trisha Miller , Jiang Xiaoyin “Sara”
Published: December 2018

We present a case of phaeohyphomycosis clinically presenting as malignancy for which fine needle aspiration provided a rapid diagnosis, with an emphasis on cytomorphology and the utility of ancillary stains. Additionally, we provide educational questions which include diagnostic methods for and clinical significance of this entity.
Termitariophily: expanding the concept of termitophily in a physogastric rove beetle (Coleoptera: Staphylinidae)
Authors: Pisno Raul M. , Salazar Karen , Lino‐Neto José , Serrão José E. , DeSouza Og
Published: December 2018

1. Termitophily in some rove beetles is commonly attributed to the striking termite worker resemblance that is provided by the beetles' hypertrophic (‘physogastric’) abdomen. However, a termite nest may offer to a termitophile additional benefits, such as a continuously repaired shelter.

2. This could apply toCorotoca melantho(Aleocharinae: Corotocini), a viviparous obligatory termitophile staphylinid beetle species. While conferring morphological congruence to its host worker termites, its physogastry may impair mobility, leading to vulnerability and the need for a secure environment. It seems plausible to hypothesise that physogastry inC. melanthowould imply in interactions between this termitophile and its host termites as well as its host termitarium.

3. This study provides evidence to build such a hypothesis by inspecting the morpho‐anatomical reproductive traits of this termitophile. It was found that a gradient of growth stages of embryos and larvae in the oviduct explains physogastry in females while pointing to iteroparity. The asynchronous development of oocytes in females, combined with a full developing sequence of sperm cells indicative of continuous spermatogenesis in males, suggests frequent matings.

4. While improving guest–host similarity, physogastry and flightlessness should confer vulnerability to pregnant females, forcingC. melanthoto seek close and sheltered environments. These could facilitate the frequent male–female contacts demanded by iteroparity. It is possible, therefore, that physogastry inC. melanthois not only associated with the termites themselves but also with the physical structure of the termitarium. Thus,C. melanthocan be hypothesised to be a termitariophile in addition to being a termitophile.
Automatic detection of violations of statistical regularities in the periphery is affected by the focus of spatial attention: A visual mismatch negativity study
Authors: File Domonkos , Czigler István
Published: December 2018

We investigated the effect of spatial attention on an event‐related potential signature of automatic detection of violations of statistical regularities, namely, the visual mismatch negativity (vMMN). To vary the task‐field and the location of vMMN‐related stimulation, in the attentional field the stimuli of a tracking task with a steady and a moving (target) bar were presented. The target stimuli of the task appeared either relatively close or far from a passive (task‐irrelevant) oddball or equiprobable sequence at the lower part of the screen. Stimuli of the oddball sequence were shapes tilted either 45° (standard, p = 0.8) or 135° (deviant, p = 0.2), while the equiprobable sequence consisted of additional three shapes with identical number of lines to the oddball stimuli. Deviant stimuli in close proximity to a continuously attended field elicited larger vMMN than similar stimuli farther away from the stimulus field. In the condition with a smaller distance between the field of the tracking task and the vMMN‐related field, the deviant stimuli and the vMMN was followed by a posterior positivity. According to these results, spatial attention modulates vMMN and is capable of initiating further processing of the deviant stimuli.
One strategy does not fit all: determinants of urban adaptation in mammals
Authors: Santini Luca , González‐Suárez Manuela , Russo Danilo , Gonzalez‐Voyer Alejandro , von Hardenberg Achaz , Ancillotto Leonardo
Published: December 2018

Urbanisation exposes wildlife to new challenging conditions and environmental pressures. Some mammalian species have adapted to these novel environments, but it remains unclear which characteristics allow them to persist. To address this question, we identified 190 mammals regularly recorded in urban settlements worldwide, and used phylogenetic path analysis to test hypotheses regarding which behavioural, ecological and life history traits favour adaptation to urban environments for different mammalian groups. Our results show that all urban mammals produce larger litters; whereas other traits such as body size, behavioural plasticity and diet diversity were important for some but not all taxonomic groups. This variation highlights the idiosyncrasies of the urban adaptation process and likely reflects the diversity of ecological niches and roles mammals can play. Our study contributes towards a better understanding of mammal association to humans, which will ultimately allow the design of wildlife‐friendly urban environments and contribute to mitigate human‐wildlife conflicts.
Infections and risk of epilepsy in children and young adults: A nationwide study
Authors: Ahlers Frederik S. , Benros Michael E. , Dreier Julie W. , Christensen Jakob
Published: December 2018

Objective

The development of epilepsy has been linked to infections of the central nervous system, but recently also to infections and inflammation outside of the central nervous system. Thus we investigated the association between infections and the risk of subsequent epilepsy.

Methods

This was a Danish nationwide population‐based cohort study comprising a total of 1 938 555 individuals born between 1982 and 2012. Individuals were followed from birth until December 31, 2012, death, disappearance, emigration, or epilepsy diagnosis, whichever came first (28 512 666 person‐years of follow‐up). The exposure was hospital contacts for infection and the outcome was a diagnosis of epilepsy as recorded in the Danish National Hospital Register. Hazard ratios were calculated using Cox proportional hazards models adjusted for age, sex, calendar period, Apgar score, gestational age, birth weight, and parental history of epilepsy.

Results

A total of 25 825 individuals received an epilepsy diagnosis during the study period, among whom 8235 (32%) had a previous hospital contact for infection. A hospital contact for infection was associated with a 78% increase in the risk of subsequently receiving an epilepsy diagnosis (hazard ratio 1.78, 95% confidence interval [CI] 1.73‐1.83) compared with those without infection. The highest risk was observed after central nervous system infections (hazard ratio 4.97, 95% CI 4.42‐5.59), but increased risks were identified across all infected organ systems and types of pathogens. The risk of receiving an epilepsy diagnosis was correlated with the temporal proximity of the infection (P < 0.001) and increased with the number of hospital contacts for infection (P < 0.001) and with the severity of infection (P < 0.001).

Significance

The risk of receiving an epilepsy diagnosis was increased after a wide range of infections, suggesting that systemic inflammatory processes may be involved in the development of epilepsy.
Habitat use and hybridisation between the Rocky Mountain sculpin (Cottussp.) and slimy sculpin (Cottus cognatus)
Authors: Rudolfsen Tyana , Ruppert Jonathan L. W. , Taylor Eric B. , Davis Corey S. , Watkinson Douglas A. , Poesch Mark S.
Published: December 2018

Anthropogenic factors such as land‐use change, pollution, and climate change can cause fragmentation and reduce the amount of habitat by altering preferred conditions. This process can also bring about novel species interactions and, in some cases, create or alter levels of hybridisation between closely related species. We assessed the threat of hybridisation to the persistence of the Rocky Mountain sculpin (Cottussp.) and the slimy sculpin (Cottus cognatus) in the Flathead River, British Columbia, Canada.

Individuals collected from 95 sites provided 731 genetic samples that were genotyped at 10 polymorphic microsatellite loci and mitochondrial cytochrome C oxidase sequences, to assess: (a) if there are differences in the distribution of Rocky Mountain sculpin between contemporary and historical (35 years ago) records, (b) if hybridisation is symmetrical in terms of sex‐specific parental contributions, and (c) if habitat preferences contribute to the distribution of pure parental and hybrid populations.

We identified three hybrid locations and that Rocky Mountain sculpin have an altitudinal distribution (1,200–1,902 m) that exceeds the range limit reported 35 years ago (1,200–1,372 m). Additionally, hybrid mating appears to involve similar proportions of parents of both sexes from each species. Lastly, elevation, turbidity, and sediment type are significant factors predicting the presence of parental species. Further, elevation and water conductivity are significant factors to predicting hybrid presence.

The contrasting associations of parental species with different habitat types appear to influence the extent and distribution of hybridisation. Our results suggest that Rocky Mountain sculpin (a species of conservation concern) do not appear to be at risk due to hybridisation, but that this is a system in flux. Given the importance of environmental conditions to hybridisation, there is a need to quantify how environmental changes may disrupt current hybridisation and potential population viability.
Watershed‐level brook trout genetic structuring: Evaluation and application of riverscape genetics models
Authors: Nathan Lucas R. , Welsh Amy B. , Vokoun Jason C.
Published: December 2018

Stream connectivity promotes resilience and population viability of aquatic organisms. Landscape genetic approaches, traditionally applied to terrestrial systems, may reveal important watershed‐level dynamics that influence connectivity. Additional validation would improve understanding of how the models perform when gene flow is constrained to dendritic networks. The objectives of this study were to use simulations to assess the utility of landscape genetics analyses in dendritic stream networks and investigate riverscape variables influencing gene flow among brook trout Salvelinus fontinalis populations in headwater streams.

We used an individual‐based simulation program to simulate different dispersal scenarios and used combinations of landscape genetic models, model selection methods, and genetic metrics to determine the best combination for riverscape systems. We also sampled brook trout from 76 headwater streams in two watersheds (c. 1,000 km2) in Connecticut, U.S.A. to assess connectivity and riverscape influences on genetic structuring.

Gravity models with Bayesian information criterion (BIC) selection were the most accurate (>85%) landscape genetic models that consistently identified the correct simulated gene flow barrier. However, all models performed poorly when unidirectional barriers were simulated without a distance‐based dispersal limitation. Excluding this scenario, model accuracy for the gravity models using BIC selection was >90% across multiple genetic metrics, validating the application of landscape genetic models to riverscape systems.

We found highly variable levels of brook trout genetic connectivity (FST range 0.01–0.19) at the watershed level (5–15 river km). Gravity models identified increases in upstream impervious surfaces and decreases in riparian tree canopy cover as riverscape variables associated with increases in genetic differentiation in one watershed, while the other watershed was consistent with an isolation by distance pattern.

In this study we used demogenetic (i.e. combined demographic and genetic) simulations to demonstrate the utility of landscape genetics techniques in dendritic river networks. Our empirical genetic study documented gene flow among headwater populations of brook trout at the watershed level and also suggested connectivity can be limited by watershed development. Incorporating the heterogeneity of riverscapes into connectivity‐focused conservation planning is essential to the development of effective restoration actions, and landscape genetics approaches can be useful tools to identify watershed‐level connectivity in stream systems.
Hydrographic correlates of within‐river distribution and population genetic structure in two widespread species of mountain galaxias (Teleostei, Galaxiidae) in southern Australia
Authors: Cook Benjamin D. , Kennard Mark J. , Adams Mark , Raadik Tarmo A. , Real Kathryn , Bunn Stuart E. , Hughes Jane M.
Published: December 2018

Closely related species may have different patterns of distribution and spatial population structure in common landscapes, which can have implications for conservation of the species if they are vulnerable. The Australian mountain galaxias species complex has recently been split into 15 described species, 11 of which are threatened. In this study, we assessed the distribution and landscape genetic structure of two mountain galaxias species (Galaxias olidus and Galaxias oliros) in the Goulburn–Broken catchment (Murray–Darling Basin) in relation to hydrographic descriptors of river terrain, network and hydrology. We also assessed small‐scale population structure in juvenile G. oliros in tributary streams of the Goulburn River (the Granite Creeks).

Analyses of distribution revealed that G. olidus mostly occurs in smaller streams at high elevations with steep valley slopes and high stream gradients downstream, whereas G. oliros mostly occurs in larger downstream waterways with higher magnitude and variability of stream flow.

Population genetic structure in G. olidus was much greater than for G. oliros, with 17 of the 19 sampled locations resolved as genetically distinct populations for G. olidus, but only a single population revealed for G. oliros. Results for both species did not reflect the expectations of the stream hierarchy model or isolation‐by‐distance model. However, small‐scale spatial population structure in juvenile G. oliros in the Granite Creeks reflected the expectations of both stream hierarchy and isolation‐by‐distance models, suggesting that population structure of juveniles of this species reflects some level of spatial aggregation of closely related individuals that probably diminishes as the population ages.

Higher landscape genetic structure in G. olidus was correlated with steeper stream and valley slopes, whereas no population genetic correlations were significant for G. oliros. Genetic diversity in G. olidus was higher in areas of greater hydrological variability, suggesting that populations in hydrologically variable areas function as a metapopulation and have a more diverse gene pool than populations in areas with lower hydrological variability. In contrast, genetic diversity in G. olidus was lower in higher elevation areas with steep valley slopes, suggesting that smaller but more stable populations occur in high elevation streams with high perenniality and low interannual variability of flows.

Our results are consistent with other studies that highlight the importance of supporting natural hydrological regimes and natural patterns of hydrological connectivity for conservation of vulnerable freshwater fish.
Soil organic carbon stability in forests: Distinct effects of tree species identity and traits
Authors: Angst Gerrit , Mueller Kevin E. , Eissenstat David M. , Trumbore Susan , Freeman Katherine H. , Hobbie Sarah E. , Chorover Jon , Oleksyn Jacek , Reich Peter B. , Mueller Carsten W.
Published: December 2018

Rising atmospheric CO2 concentrations have increased interest in the potential for forest ecosystems and soils to act as carbon (C) sinks. While soil organic C contents often vary with tree species identity, little is known about if, and how, tree species influence the stability of C in soil. Using a 40 year old common garden experiment with replicated plots of eleven temperate tree species, we investigated relationships between soil organic matter (SOM) stability in mineral soils and 17 ecological factors (including tree tissue chemistry, magnitude of organic matter inputs to the soil and their turnover, microbial community descriptors, and soil physicochemical properties). We measured five SOM stability indices, including heterotrophic respiration, C in aggregate occluded particulate organic matter (POM) and mineral associated SOM, and bulk SOM δ15N and ∆14C. The stability of SOM varied substantially among tree species, and this variability was independent of the amount of organic C in soils. Thus, when considering forest soils as C sinks, the stability of C stocks must be considered in addition to their size. Further, our results suggest tree species regulate soil C stability via the composition of their tissues, especially roots. Stability of SOM appeared to be greater (as indicated by higher δ15N and reduced respiration) beneath species with higher concentrations of nitrogen and lower amounts of acid insoluble compounds in their roots, while SOM stability appeared to be lower (as indicated by higher respiration and lower proportions of C in aggregate occluded POM) beneath species with higher tissue calcium contents. The proportion of C in mineral associated SOM and bulk soil ∆14C, though, were negligibly dependent on tree species traits, likely reflecting an insensitivity of some SOM pools to decadal scale shifts in ecological factors. Strategies aiming to increase soil C stocks may thus focus on particulate C pools, which can more easily be manipulated and are most sensitive to climate change.
Trade and indeterminacy revisited
Authors: Iwasa Kazumichi , Nishimura Kazuo , Yano Makoto
Published: December 2018

We consider a dynamic two‐country model of trade with production externalities with an emphasis on the possibility of global indeterminacy, which means that the distribution of capital stocks in each country in the long run depends on households’ expectations. Opening trade yields expectation‐driven fluctuations. Global indeterminacy may occur around the free trade steady states even when the autarkic steady states are saddle points in both countries, and it must happen when local indeterminacy occurs around the autarkic steady states in both countries.
Bacteremia complicating urinary tract infection by Pseudomonas aeruginosa: Mortality risk factors
Authors: Kitagawa Koichi , Shigemura Katsumi , Yamamichi Fukashi , Osawa Kayo , Uda Atsushi , Koike Chihiro , Tokimatsu Issei , Shirakawa Toshiro , Miyara Takayuki , Fujisawa Masato
Published: December 2018

Objectives

To examine the clinical risk factors for death within 30 days of diagnosis of Pseudomonas aeruginosa‐causing bacteremia after a urinary tract infection.

Methods

A total of 62 patients with Pseudomonas aeruginosa isolated from both urine and blood at the same episode from January 2009 to December 2016 were enrolled in the present study. We retrospectively investigated clinical risk factors for death by comparison between surviving patients and those who died within 30 days after diagnosis of P. aeruginosa bacteremia. The comparison for risk factors for bacteremia‐related death included 31 categories, such as age, laboratory data, underlying diseases, clinical history, history of surgery, care in the intensive care unit, P. aeruginosa susceptibility to the antibiotics used at the time of bacteremia diagnosis and consultation with urological department.

Results

The study included 48 men and 14 women aged 71.3 ± 10.4 years. Nine patients (14.5%) died of P. aeruginosa bacteremia. Statistical analysis showed that non‐survivors had significantly lower albumin levels than survivors (2.07 ± 0.62 vs 2.62 ± 0.65; P = 0.023). The non‐survivors had significantly higher rates of ventilator use, history of heart disease, septic shock and lower rates of consultation with urological departments after diagnosis (P < 0.05).

Conclusions

Patients with bacteremia complicating urinary infection by P. aeruginosa have a low death rate. Earlier intervention by urologists might improve patients’ outcome. Lower albumin levels, ventilator use, history of heart disease and septic shock are factors associated with higher mortality rate.
Angiographic management of percutaneous renal procedure‐related bleeding: A single‐center experience
Authors: Choi Min Jeong , Kim Pyeong Hwa , Shin Ji Hoon , Kim Jong Woo , Gwon Dong Il , Kim Jin Hyoung , Ko Gi‐Young , Yoon Hyun‐Ki , Ohm Joon Young
Published: December 2018

Objectives

To present the radiological and clinical results of transcatheter arterial embolization in patients with active bleeding after percutaneous renal procedures.

Methods

A total of 79 consecutive patients who underwent angiography for percutaneous renal procedure‐related bleeding were included in the present retrospective analysis. Patient characteristics, angiographic management and clinical outcomes were analyzed.

Results

On angiography, bleeding foci were observed in 81.0% of the patients (64/79), all of whom underwent transcatheter arterial embolization. Among the 15 patients (19.0%) with negative angiographic findings, empirical transcatheter arterial embolization was carried out in six patients (40.0%). The technical success rate in 64 patients with positive angiographic findings was 100%, and the clinical success rate in 70 patients who underwent transcatheter arterial embolization was 85.7% (60/70). A total of 14.3% (10/70) of patients with clinical failure underwent repeat transcatheter arterial embolization and all achieved clinical recovery. There were no major complications. There was no statistical difference in estimated glomerular filtration rate values before the percutaneous renal procedure and those measured 7 days after transcatheter arterial embolization (43.4 ± 24.4 to 44.6 ± 25.1 mL/min/1.73 m2; P = 0.189). Clinical failure was not associated with age, sex, type of renal procedures, bleeding tendency, presence of active bleeding on angiography, latency time and embolic agents used (P > 0.05).

Conclusions

Transcatheter arterial embolization is a safe and effective method of treating percutaneous renal procedure‐related bleeding without renal function deterioration.
Experiences of self‐stigmatization and parenting in Chinese mothers with severe mental illness
Authors: Chan Sonia Ying Yin , Ho Grace W.K. , Bressington Daniel
Published: December 2018

Mental health stigma has serious ramifications on people with a severe mental illness (SMI). Stigma damages self‐esteem, recovery outcomes, family relationships, socialization abilities, access to housing, and career prospects. The cultural tendencies of Chinese people have been shown to be associated with particularly high levels of stigmatization. These cultural tenets can result in high levels of self‐stigma due to experiencing shame and a perceived need to keep mental illness a secret. Although there is a lack of existing evidence, it is possible that such experiences present unique challenges to Chinese mothers diagnosed with SMI when they parent their children. Therefore, this qualitative study explored the experiences of parenting and self‐stigmatization of Chinese mothers with SMI. Individual semi‐structured interviews were conducted with 15 mothers who were direct carers of their children aged under 18 and who were receiving community‐based care in Hong Kong. Manual inductive thematic analysis was used to analyse the interview data. Three main themes related to self‐stigmatization emerged from the interviews: (i) distancing and being distanced; (ii) doubting myself; and (iii) struggling for control. The experiences of self‐stigmatization appeared to damage these mothers’ self‐efficacy, which may negatively affect their parenting self‐esteem, tendency to seek professional help, and ability to manage their own mental health. Strategies to improve self‐efficacy, including psychoeducation, and additional childcare support/resources are required for mothers with SMI so they can better manage and balance the demands of motherhood and their mental healthcare needs.
Influenza B associated paediatric acute respiratory infection hospitalization in central vietnam
Authors: Yoshihara Keisuke , Le Minh Nhat , Toizumi Michiko , Nguyen Hien Anh , Vo Hien Minh , Odagiri Takato , Fujisaki Seiichiro , Ariyoshi Koya , Moriuchi Hiroyuki , Hashizume Masahiro , Dang Duc Anh , Yoshida Lay‐Myint
Published: December 2018

Background

Influenza B is one of the major etiologies for acute respiratory infections (ARI) among children worldwide; however, its clinical‐epidemiological information is limited. We aimed to investigate the hospitalization incidence and clinical‐epidemiological characteristics of influenza B‐associated paediatric ARIs in central Vietnam.

Methods

We collected clinical‐epidemiological information and nasopharyngeal swabs from ARI children hospitalized at Khanh Hoa General Hospital, Nha Trang, Vietnam from February 2007 through June 2013. Nasopharyngeal samples were screened for 13 respiratory viruses using Multiplex‐PCRs. Influenza B‐confirmed cases were genotyped by Haemagglutinin gene sequencing. We analyzed the clinical‐epidemiological characteristics of influenza B Lineages (Victoria/Yamagata) and WHO Groups.

Results

In the pre‐A/H1N1pdm09 period, influenza B‐associated ARI hospitalization incidence among children under five was low, ranging between 14.7 and 80.7 per 100 000 population. The incidence increased to between 51.4 and 330 in the post‐A/H1N1pdm09. Influenza B ARI cases were slightly older with milder symptoms. Both Victoria and Yamagata lineages were detected before the A/H1N1pdm09 outbreak; however, Victoria lineage became predominant in 2010‐2013 (84% Victoria vs 16% Yamagata). Victoria and Yamagata lineages did not differ in demographic and clinical characteristics. In Victoria lineage, Group1 ARI cases were clinically more severe compared to Group5, presenting a greater proportion of wheeze, tachypnea, and lower respiratory tract infection.

Conclusions

The current results highlight the increased incidence of influenza B‐related ARI hospitalization among children in central Vietnam in the post‐A/H1N1pdm09 era. Furthermore, the difference in clinical severity between Victoria lineage Group1 and 5 implies the importance of influenza B genetic variation on clinical presentation.