bluuebunny/crossref_metadata_2025 huggingface dataset:
Zymo Duet DNA/RNA MiniPrep Plus Extractions v1
Authors: Lyden Amy
Published: February 2019

Attached are detailed instructions for following the kit guidelines for the Zymo Duet DNA/RNA MiniPrep Plus.
SEM Sample Prep v1
Authors: D'angelo Tim , N Orcutt Beth
Published: February 2019

The SEM can be used to directly image biofilms on solid surfaces such as polished rock chips or collected crustal samples. Seawater or fluid bacteria can be imaged after filtering the sample onto a 0.2 μM filter.
Disabled Mothering? Outlawed, Overlooked and Severely Prohibited: Interrogating Ableism in Motherhood
Authors: Daniels Julia N.
Published: February 2019

The ideology of motherhood precludes disabled people in various ways: sometimes outlawing it completely, in the case of enforced or coerced sterilisation; sometimes condemning it through the sanctioned removal of children and/or adoption; and at other times complicating it severely through lack of access to accessible goods and services that all mothers require to function in their day-to-day lives—such as pushchairs/prams, baby-changing equipment and baby-wearing apparatus. Ableism, “compulsory able-bodiedness” (Campbell, 2009; McRuer, 2013), will be used as an interrogative tool to aid in the ‘outing’ of the ‘able’: to tease out the values and principles undergirding this exclusionary perception of motherhood. As such I will be drawing on autoethnographic material, in conjunction with a Studies in Ableism (SiA; Campbell, 2009) approach to analyse the bypassing of disabled mothers and to suggest tentative ways forward. In the UK 1.7 million parents identify as disabled (Morris & Wates, 2006) and perhaps many more would do so if there were no fear of censure (see, especially, Booth & Booth, 2005; Llewellyn, McConell, & Ferronato, 2003; Sheerin, 2001; Swain, French, & Cameron, 2003) and their requirements need to be recognised, heard and provided for in the consumer market. The following article will articulate how disabled mothers are barred from the sacred hallow of motherhood, and delineate the need for the media, governmental organisations and marketing corporations to address their culpability in this blatant discrimination.
Distribution of malignant neoplasms among patients with endometriosis: an epidemiological study
Authors: Bairamova Nurana N. , Protasova Anna E. , Raskin Grigory A. , Yarmolinskaya Maria I. , Tsypurdeyeva Anna A. , Gavrilova Maria D.
Published: February 2019

Hypothesis/aims of study. The current analysis was undertaken to assess the incidence of malignant diseases in patients with endometriosis.

Study design, materials and methods. This is a cohort study of women with surgically verified endometriosis retrieved from the Saint Petersburg 122 Clinical Hospital Discharge Register 1996–2006 (n = 1551). Data were linked to the Saint Petersburg Cancer Agency to identify cases of malignancy.

Results. Malignant diseases were identified in 6.3% (n = 98) of cases. The median follow-up was 12.2 (7.5) years. Breast cancer (n = 29), ovarian cancer (n = 7), endometrial cancer (n = 7), thyroid cancer (n = 7), colorectal cancer (n = 6), melanoma (n = 6), non-Hodgkin’s lymphoma (n = 4), and glioblastoma (n = 3) were prevalent.

Conclusion. The incidence of cancer in patients with endometriosis is low. Women with endometriosis have an increased risk of some malignancies, particularly ovarian cancer.
Design, synthesis and biological evaluation of 1,3-diaryltriazenesubstituted sulfonamides as antioxidant, acetylcholinesterase and butyrylcholinesterase inhibitors
Authors: AKOCAK Süleyman , BOGA Mehmet , LOLAK Nabih , TUNEG Muhammed , SANKU Rajesh K K
Published: February 2019

1,3-diaryltriazenes are one of the most useful and
important linkers for many pharmaceutical applications. Therefore, in the
current work, a series of 1,3-diaryltriazene sulfonamides 4(a-k) were synthesized by reacting diazonium salt of sulfanilamide
and substituted aromatic amine derivatives 3(a-k).
The obtained compounds were investigated for antioxidant properties by using
different methods such as a DPPH radical scavenging assay, ABTS radical
decolarization, cupric reducing antioxidant capacity (CUPRAC) and metal
chelating methods. The cholinesterase inhibition activities
(acetylcholinesterase and butyrylcholinesterase) of synthesized compounds were
also tested. In general, compounds showed weak antioxidant activity, except
compounds 4d (IC50
=114.89 for DPPH activity), 4i (IC50
=25.31 for ABTS activity), 4a (IC50
= 86.33 for metal chelating activity), and 4k
(absorbance value 1.229 µM for CUPRAC). Some of the compounds showed great %
inhibition against both acetylcholinesterase and butyrylcholinesterase with %
inhibition values ranging from 11.54 to 93.67 and 62.24 to 98.47, respectively.
The impact of nutritional status on the outcomes of cancer patients such as mortality, survey and length of hospitalization in palliative care
Authors: AVCI Deniz , Çetinkaya Ali
Published: February 2019

Objectives: The aim of this study was to determine the effects of
nutritional status of the cancer patients in the palliative unit on some
important outcomes such as mortality, hospitalization periods and survey. Methods: The study was carried out through a retrospective
review of 65 cancer patients who were treated at the palliative care center.
The age and gender of the patients, the type of cancer, the place where they
are referred to the palliative unit (from home or from the hospital), from
which unit they came from (oncology, intensive care unit, other services), the
length of stay, how long they lived after discharge, how long each patient
lived after the palliative unit hospitalization day and the NRS-2002 scores
were recorded. Results: There was a statistically significant difference
between the median hospital duration of the home-based patients and the median
duration of hospital-based patients (11 [2-42] days versus 22 [2-180] days) (p = 0.001). The mean survival time of
the home-based patients was median 87.5 (2-323) days, while this was 9 (2-104)
days in hospital-based patients (p =
0.017). While 29.5% (n = 13/27) of the patients coming from the house died in
the palliative care center, it was 70.5% in the patients taken from the
hospital (p = 0.002). The NRS-2002
scores of the cancer patients who were followed up at the palliative unit were
correlated with the age of the patients (r = 0.365, p = 0.003).

Conclusions:
We concluded that the patients who came to palliative care from home have
better surveys than the ones came from the hospital.
Pengaruh Suhu Dan Kepadatan Ephippia Yang Berbeda Terhadap Penetasan Ephippia Daphnia magna [The Influence Of Defferent Temperature And Ephippia Density For Ephippia Hatch Of Daphnia magna.]
Authors: Cahyoko Yudi , Eka Pradana Yulian Cindra , Rahardja Boedi Setya
Published: February 2019

Abstract Daphnia magna has good nutrition to carry on egg produce and fry of fish cupang, maskoki, oscar, tetra, and also can used for food source of fry and seed. Daphnia magna not only supply from natural environment, cause their growth deppend on fertilizer as food and environment condition. The other side: high population density, limited of food source low temperature and shorter irradiation can produce dorman egg (ephippia). The dorman eeg can use for Daphnia magna stock which way by hatching. The factor that can be influence for ephippia hatch are temperature and density. Based on this fact, a research about influence of different temperature and ephippia density for ephippia hatch of Daphnia magna. The purpose of this research was to know the influence of different temperature and ephippia density for ephippia hatch of Daphnia magna. This research has experimental characteristic and use Factorial Completely Randomized Design with three experiments of temperature (A factor), three experiments of density (B factor) and three times replicated. There are 9 kinds of combination between density and temperature. The parameter which checked in this research is degree of hatch of ephippia Daphnia magna and water quality that is dissolve oxygen rate, NH3 and of pH hatch media. The result of the analysis showed that the temperature and density influence of hatching Daphnia magna ephippia. The best result can be used for hatching Daphnia magna ephippia is temperature at 25oC with density 50 ephippia /100 ml giving highest result for the hatch of ephippia Daphnia magna. There are interaction betwen temperature and density for hatching.
Pengkayaan Daphnia spp. Dengan Viterna Terhadap Kelangsungan Hidup Dan Pertumbuhan Larva Ikan Lele Dumbo (Clarias gariepinus) [Enrichment Of Daphnia spp. With Viterna To Survival And Growth Of African Catfish (Clarias gariepinus) Larvae]
Authors: Rahardja Boedi Setya , Satyantini Woro Hastuti , Mufidah Naila Budiatin Wahyu
Published: February 2019

Abstract African catfish (Clarias gariepinus) is one of fish consumption use to fulfill animal protein. The fulfillment need of animal protein with aquaculture effort, where as in the aquaculture effort will larvae quality increased. Increasingly of larvae quality with natural food had been adding feed supplement. Natural food to used is Daphnia spp. and then food supplement to used is viterna. The viterna will increase Daphnia nutritions, because of the viterna contains many nutritions to survival and growth of African catfish. The aim of this study was to know the influence of Daphnia spp. enrichment with viterna to survival and growth of African catfish (Clarias gariepinus) larvae. The rearing of larvae during 40 days. This study used Complete Random Design. The treatment were A (present Daphnia spp. without viterna), B (Daphnia spp. enrichment with viterna 10 ml/L dose), C (Daphnia spp. enrichment with viterna 10 ml/L dose), D (Daphnia spp. enrichment with viterna 10 ml/L dose), E (Daphnia spp. enrichment with viterna 10 ml/L dose) dan F (Daphnia spp. enrichment with viterna 10 ml/L dose), each treatment with 4 replications. The result show that viterna applications to Daphnia was significantly influenced (p<0,05) to survival African catfish larvae. The highest survival was E treatment (7,25 %). The viterna leaf to Daphnia spp. Was significantly influenced (p<0,05) to specific growth rate African catfish larvae. The highest specific growth rate was B treatment (1,885 %BW/day).
Pemberian Probiotik Pada Pengolahan Limbah Udang Yang Dimasak dengan Tekanan Tinggi Terhadap Kandungan Protein Kasar dan serat Kasar [Giving Probiotic At Prawn Waste Processing With High Pressure Cooked for Crude Protein and Crude Fibre Content]
Authors: Agustono Agustono , Handyani Ika Agustin , Lamid Mirni
Published: February 2019

Abstract Feed is one of the factors determines the success of a fish farm. Feed-quality feed stuffs that require high quality also, which still rely on imports, especially fish meal and soybean oilcake. Effort to reduce dependence on import of feed stuff is searching for alternative feed stuffs that the quality is quite good, cheap, easily obtained and can reduce cost of production. One of the feed stuff as an alternative source of animal protein feed that need to be examined is the prawn waste. Prawn waste meal enough potential to be used as fish feed with a crude protein content 45.29% and 17.59% crude fiber was alternative feed stuff for fish meal. The usage of prawn waste as a feed stuff should be through the handling and processing to further improve the nutrient value of waste. Prawn waste processing in this research consists of two ways, that is waste cooking prawn on the high pressure (100 kpa), and conducted fermentation by using probiotic. The existence of microorganisms activity during the fermentation process will cause changes of feed stuffs either through physically and chemicals. Probiotic is a microbe colony that is rich in celulolytic, lignolytic and proteolytic bacteria. The aim of this research is to know the influence probiotic on the prawn waste cooked with high pressure to increase the protein content and to decrease the crude fiber content. Method as used in the research is experimental method. Experiment design that used at research is Completely Randomized Design with 4 treatments and replicates 5 times. To get the data is conducted procsimat analysis on the crude protein content and crude fibers from each experimental unit. That result data analyzed with the Analysis of Varian continued with Duncan’s Multiple Range Test. The results of research is known that dosaged probiotic at process of prawn waste cooked fermentation not different to crude protein contents of the waste cook fermented prawn. Crude fiber content of the waste cook fermented prawn using probiotik (P1, P2, P3) has decreased if compared with waste cooking fermented prawn without using probiotic (P0)
Pengaruh Kombinasi Pupuk NPK dan TSP Terhadap Pertumbuhan, Kadar Air dan Klorofil A Gracilaria verrucosa [The Influenced Of NPK and TSP Fertiliter Combination On The Growth, Water Concentration and Chlorophyll A Of Gracilaria verrucosa]
Authors: Alamsjah Moch. Amin , Tjahjaningsih Wahyu , Pratiwi Anugraheny Widaratna
Published: February 2019

Abstract Gracilaria verrucosa is one of the most potential seaweed and consist of a high economy value. G. verrucosa had been succed cultivated in pond and it produces gel (Lewmanomont, 1995). Gracilaria cultivation in pond generally needs a wide area, easily infected by moss and shellfish so that obstructs Gracilaria’s growth, even decreases it’s quality (Aslan, 1998). One of the solution to solve the problem above is cultivate another seaweed G. verrucosa indoor using the combination of NPK and TSP. NPK and TSP are used to increase growth and formation of chlorophyll a which used to fotosintesis process (Anggadiredja dkk., 2006). The absortion of hara element will add nutrient and influenced to the G. verrucosa’s quality and quantity. This research uses water concentration measuring because gel content measuring is relatively expensive. The goal of this research is knowing the influence of NPK and TSP to G. verrucosa’s growth, water concentration and quantity of chlorophyll a. The result of G. verrocosa shows that daily growth heavy of G. verrocosa in D treatment extremely different (p<0,05) with A, B, C, E and F treatments. The best daily growth heavy of G. verrocosa is in D treatment and the lowest daily growth heavy of G. verrocosa is in A treatment. The result growth length shows that in D treatment extremely different (p<0,05) with A, B, C, E and F treatment. The best growth length is in D treatment and the lowest growth length is in A treatment. The result G. verrucosa’s water concentration shows that in C, D and E treatment extremely different (p<0,05) with A, B and F treatment. The best water concentration is in C, D and E treatment. The lowest water concentration is in A, B and F treatment. The result chlorophyll a quantity shows that in B, C, D E and F treatment extremely different (p<0,05) with A treatment. The best G. verrucosa’s chlorophyll a quantity is in B, C, D, E and F treatment. The lowest G. verrucosa’s chlorophyll a quantity is in A treatment. The conclusion of this research is the combination of NPK and TSP in G. verrucosa seaweed cultivation extremely affect the growth, water concentration and chlorophyll a G. verrucosa with the best dose is 2 g/l and the ratio NPK and TSP is 50%:50%.
Pengaruh Pemberian Vaksin Whole Cell Killed Virus Terhadap Sintasan Udang Vannamei (Lithopenaeus vannamei) Yang Diinfeksi Whitespot Baculovirus (WSBV)
[The Influence Of Whitespot Baculovirus (WSBV) Infection In Lithopenaeus vannamei By Giving Whole Cell Killed Virus Vaccine On Survival Rate.]
Authors: Suprapto Hari , Siswanto Angga Bahtera , Rahardja Boedi Setya
Published: February 2019

AbstractShrimp are susceptible to a wide variety of pathogens, one of each were viruses. Whitespot Baculovirus (WSBV) is one of virus attack in vannamei. Shrimp had been infected by WSBV will showed high mortality. One of Strategies for prophylaxis and control of WSBV is enhancement of disease resistant by using vaccines. Recently, quasi-immune response have been reported by which Penaeus japonicus surviving from WSBV infections possess a resistance against challenge WSBV (Venegas et al., 2000). This study was used Lithopenaeus vannamei that been vaccination with inactived-formaline WSBV virus cell or it called Whole Cell Killed Virus (WCKV) to know its responses to WSBV. The aim of this study was to know the effect Whole Cell Killed Virus vaccine to survival rate vannamei shrimp (Lithopenaeus vannamei) infected by Whitespot Baculovirus (WSBV). This study used descriptive study. The efficacy of vaccines made of inactivated WSSV with different dose. The dose are P1 (dose 0,01µg/ml each shrimp), P2 (dose 0,02 µg/ml each shrimp), P3 (dose 0,03 µg/ml each shrimp) dan P4 (non-vaccine (kontrol) injected by PBS). Primary parameter was survival rate (%). Secondary parameter was water quality which of temperature, pH, salinity and dissolved oxygen. The result showed that survival rate of L. Vannamei with different dose of WCKV vaccines was increases.
Daya Antibakteri Ekstrak Buah Adas (Foeniculum vulgare) Terhadap Bakteri Micrococcus luteus Secara In Vitro
[Antibacterial Effort Of Adas Fruit (Foeniculum vulgare) Extract On Micrococcus luteus Bacterial By In Vitro]
Authors: Kusdarwati Rahayu , Sari Ludira , Subekti Sri
Published: February 2019

AbstractOne of the disease attack of freshwater fish is micrococcus which caused by Micrococcus luteus bacterial. Antibiotic was caused of residual, polluted and expensive. Therefore, it was looked for alternative using natural and safety materials. The study used the fruit extract of adas as antibacterial to Micrococcus luteus. This experiment purpose to investigate of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of fruit extract of adas to Micrococcus luteus. The study was done on 15 June-05 July 2009 on Development Center of Quarantine Fish Juanda; Institute Tropical Disease, Airlangga University Surabaya; and Department of Faculty Pharmacology of Medicine, Airlangga University Surabaya. The experiment was used experimental method which completely randomized design with twelve treatments and three replicates. The parameters observed were Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The data analyzed used F test and was continued with Duncan's Multiple Range Test. The result indicated that fruit extract of adas had antibactericidal to Micrococcus luteus. According to  inhibitory test growth of bacterial had inhibitory capacity (MIC) at concentration 1,56% (0,018 g/ml). Therefore, the bactericidal capacity (MBC) was at concentration 3,13% (0,036 g/ml).
Teknik Budidaya Rumput Laut (Kappaphycus alvarezii) dengan Metode Rakit Apung di Desa Tanjung, Kecamatan Saronggi, Kabupaten Sumenep, Jawa Timur
[Technique Culture of Sea Weed (Kappaphycus alvarezii) with Flouting Raft Method in Tanjung Village, Saronggi Sub District, Sumenep Regency, East Java]
Authors: Abdullah Annur Ahadi
Published: February 2019

Abstract Sea weed is a great culture commodity with high economical value. The purpose of this Field Job Practice were to get knowledge and skill ,the constraint, and also the opportunity to develop it. This Field Job st thPractice performed in Tanjung Village at July 31 until September 10  2006 used description method. Sea weed culture techique use flouting raft with raft size 7 x 10 m and string distance 14 cm  with culture 30 days period. The constraint  are pest attack like Baronang (Siganus sp.), ice ice, white spot disease and also weather fluctuation.This culture has opportunity to develop because  it has fast cash back about 2,176 periode.
Efektivitas Alat Tangkap Mini Purse Seine Menggunakan Sumber Cahaya Berbeda Terhadap Hasil Tangkap Ikan Kembung (Rastrelliger sp.)
[Fishing Tool Effectivity of Mini Purse Seine by Using Different Light Source for Catching Fish of Puffer Fish (Rastrelliger sp.) ]
Authors: Farid Akhmad , Nugraha Wahyu Andy , Rosyidah Ifa Nur
Published: February 2019

AbstractIn fish catching activity, the fishermen in Banyuanyar district use mini tools for catching fish (purse seine) and use light assist tools for an operation in the night day. The light, that is used, is kerosene pressure lantern and mercury lights or lamp that is used on water surface (surface lamp). This kind of light is used to collect “pelagic” fish that has positive phototaxis characteristic. Puffer fish's response towards different sourse of light, that's kerosene pressure lantern and mercury light is needed to be known. Therefore, we can know the source of light that is more effective in order to collect the fish. It is hoped that the productivity of puffer fish will be developed for the fishermen. During the research with ten times repeating, the total account of puffer fish's haul that is resulted with two different treatment  by using kerosene pressure lantern and mercury light is that 810 kg and 1.460 kg. The data of puffer fish's haul that is gotten after using MannWhitney test shows that the kerosene pressure lantern and mercury light not to really different, especially in the haul of puffer fish in significant standart (0,05) is 2,262, while in Mann-Whitney test account is 0,171. That means the fishermans in Banyuanyar can use kerosene pressure lantern and mercury light for catching fish as the light assist tools for an operation in the night day.
Strategi Bakteri Probiotik untuk Menekan Pertumbuhan Bakteri Patogen di dalam Pencernaan Kerapu Chromileptes altivelis dengan Memproduksi Beberapa Bakterial Substansi
[ Probiotic Bacteria Strategy For Depressing Patogenic Bacteria Growth In Digestion Of Grouper Fish (Chromileptes altivelis) With Producing Of Substancy Bacterial ]
Authors: Agustono Agustono , Suprapto Hari , Muhajir Muhajir
Published: February 2019

Abstract The aim of the research is looking for probiotic bacteria strategy for depresing the growth of patogenic bacteria in digestion of grouper fish. Some of anti bacteria can be produced by bacteria, such as protease, bacteriocia (germicidine, pyrocydia, circulin), amilase lipase, and lyzozym. In this case, Bacillus sp. is chosen because it can produce antibacteria againts Vibrio sp. and probiotic multifactor effect, that is enzyme production and competition of nutrient and space.
Studi Perbandingan Kemampuan Nannochloropsis sp. dan Spirulina sp. sebagai Agen Bioremediasi terhadap Logam Berat Timbal (Pb)
[Comparative Study Of Ability Nannochloropsis sp. And Spirulina sp. As Agent Bioremediation Of Heavy Metal Plumbum (Pb) ]
Authors: Masithah Endang Dewi , Rahardja Boedi Setya , Hardianie Tri Nadya Olyvia Kerin
Published: February 2019

Abstract Sea water is a component that interacts with the terrestrial environment, where the discharge of waste empties into the sea to the mainland. One of the most dangerous pollutants for human health is the heavy metal plumbum (Pb). Bioremediation offers a promising alternative method and the potential to reduce the concentration of heavy metals in water. Bioremediation is the application of biological processes to recover a contaminated place by using microorganisms. Biomass of algae Nannochloropsis sp. can be used as bioremediation of heavy metals because it has the ability adsorption caused the active cluster contained therein. In addition, Spirulina sp. thought to have the ability as an agent of bioremediation of heavy metal plumbum (Pb) because the proteins and polysaccharides are high. Information about uptake ability of heavy metal of plumbum (Pb) by Nannochloropsis sp. and Spirulina sp., in order to know how it compares to the ability of Nannochloropsis sp. and Spirulina sp. in absorbing the content of heavy metal plumbum (Pb). The method used in this study is the experimental method, the test T of SPSS analysis as the experimental design. Treatment given in the form of differences in the concentration of plumbum, which include, treatment A (Nannochloropsis sp. without the addition of plumbum), treatment B (Nannochloropsis sp. 0.9 ppm with plumbum concentrations), treatment C (Spirulina sp. without the addition of plumbum) and treatment D (Spirulina sp. with plumbum concentrations 0.9 ppm) of each treatment was repeated 5 times. The main parameters measured were real heavy metal plumbum (Pb) in water culture media Nannochloropsis sp. and Spirulinna sp. The results showed that Nannochloropsis sp. and Spirulina sp. able to absorb the heavy metals plumbum (Pb) so that it can be used as a bioremediation agent, where Spirulina sp. have higher ability in absorbing heavy metals plumbum (Pb) compared with Nannochloropsis sp
Pengaruh Pemberian Probiotik Berbeda pada Pakan Komersial terhadap Pertumbuhan Dan Efisiensi Pakan Ikan Lele Sangkuriang (Clarias Sp.) [ The Present Effect Of Different Probiotics On Commercial Feed Towards Growth And Feed Efficiency Of Sangkuriang Catfish (Clarias Sp.) ]
Authors: Arief Muhammad , Fitriani Nur , Subekti Sri
Published: February 2019

Abstract Freshwater culture commodity such as sangkuriang catfish (Clarias sp.) has high sufficient demand. One way to fullfil necessity of sangkuriang catfis demand is accelerate the growth. The aim of this research is to determine the present effect of different probiotics on commercial feed towards growth and feed efficiency of sangkuriang catfish. The research using experimental method, using Completely Randomized Design (CRD). Sangkuriang catfish kept in 30 days with four treatments and five replications, that is P1 (control), P2 (5% probiotics A), P3 (5% probiotics B), and P4 (5% probiotics C). The obtained data were processed by Analysis of Variance (ANOVA) and followed by Duncan Multiple Range Test if there was significant data. The result showed that different probiotics giving significant effect to growth rate and feed efficiency (p<0,05). The best sangkuriang catfish (Clarias sp.) growth rate is P2 treatment 2,88% ± 0,38, and the lowest is P1 treatment at 1,73% ± 0,7. The best feed efficiency is P2 treatment at 54,69% ± 9,67 and the lowest is P1 treatment at 30,27% ± 12,65
Pengaruh Padat Tebar Ikan Lele Terhadap Laju Pertumbuhan dan Survival Rate Pada Sistem Akuaponik [ The Effect Of Stocking Density On Survival Rate and Grow Rate Of Aquaponic System]
Authors: Rahardja Boedi Setya , Wijaya Ongky , Prayogo Prayogo
Published: February 2019

AbstractIncreasing consumption of catfish and dairy products encourage increased domestic production of catfish. During the period of 5 years (2005-2009), catfish production has been increased significantly with an average annual growth reached 32%. In 2008 production reached 114.371 tonnes and in 2009 production increased by almost 75% to about 200 thousand tons. Aquaponic is a bio-integration that links recirculating aquaculture principles to the production of crops / vegetables hydroponically (Diver, 2006). Aquaponic technology has proven to successfully produce an optimal fish on less land and limited water resources, including in urban areas (Ahmad, 2007). This study aims to find out the influences of catfish stocking density difference on the growth rate and survival rate of catfish on aquaponic. The experimental design used was completely randomized design (CRD). Analysis of the data processed using Analysis of Variance (ANOVA) to know whether there is influance the growth rate and survival rate between the treatment given. If there are significantly difference then proceed by Duncan's Multiple Range Test. Based on the research obtained the result that there are significant differences in the survival rate (p> 0.01) and there is a significant difference to the rate of growth (p> 0,01). From the results of statistical tests that have been done show that the highest survival rate is in treatment 4 (87.5333%) and the highest growth rate is in treatment 4 (30.5333%). The lowest Survival found in treatment 1 (53.134%). Lowest growth rate found in treatment 1 (16.6%).
Pengembangan Metode Isolasi Kondroitin Sulfat dari Tulang Rawan Sotong (Sepia phraonis), Pari (Raja sp.), Dan Hiu (Carcharinus falciformes)
[The Isolation Development of Chondroitin Sulphate from Cuttlebone (Sepia phraonis), Ray’s Cartilage (Raja sp.), and Shark (Carcharinus falciformes) ]
Authors: Alamsjah Moch. Amin , Hanindika Dimas , Sugijanto Noor Erma
Published: February 2019

Abstract Chondroitin sulfate are drugs for osteoarthritis which usually made from pig cartilage and shark , the material can not be accepted because of the prohibition of pork and rare of shark. This study developed a method of Nakano, Garnjanagoonchorn, and Volpi . Extraction and isolation of chondroitin sulfate from alternative raw materials such as the rays and cuttlefish cartilage materials because both are abundant and cheap. Isolation results were analyzed using Fourier Transform - Infrared and Thermal Analysis diffrential, obtained 2.37% chondroitin sulfate from shark cartilage, 1.57 % from ray’s, while the squid was not obtained chondroitin sulfate.
Aplikasi Teknologi Asap Cair dalam Pengolahan dan Pengawetan Produk Perikanan di Pulau Mandangin [ Technology Application of Liquid Smoke in Processing and Preservation of Fishery Products in The Mandangin Island]
Authors: Andriyono Sapto , Thajaningsih Wahju , Agustono Agustono , Masithah Endang Dewi , Pursetyo Kustiawan tri , Abdillah Annur Ahadi , Pramono Heru
Published: February 2019

Abstract The increase in value-added of fishery products is one of which can be done by processing. In addition to maintaining the value of the protein in the fish raw material, it would be easier if the product is stored and increase the sale value of the fish itself. The ability to perform in the fish processing in Mandangin island is still very limited. Community service activities that have been carried out by a team of the Faculty of Fisheries and Marine Universitas Airlangga was introduce smoked fish product processing with liquid smoke,because this method easier and faster so that the products can be marketed. Liquid smoke was developed because the location is very minimal in the supply of raw materials such as firewood and coconut, so the use of liquid smoke is very appropriate. The resulting refined products are expected to be a source of protein Mandangin Island community itself and is able to become an alternative source of income for the community to increase the sale value of fishery products. Mentoring process being conducted by a team from the Faculty of Fisheries and Marine Universitas Airlangga so that the products can be marketed outside Mandangin Island or even to other areas in East Java.
Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus Koi terhadap Tingkat Kerusakan Usus Ikan Koi (Cyprinus carpio Koi) [ The Effectivety Crude and Soluble Protein of Myxobolus Koi Spore againts Intestine Different Degrees in Koi (Cyprinus carpio Koi)]
Authors: Mahasri Gunanti , Anggarani Rachma Woro , Suwanti Lucia Tri
Published: February 2019

Abstract Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi’s. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi’s were analyzed using the Kruskal-Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage
Pengaruh Ekstrak Alga Merah (Kappaphycus alvarezii) terhadap Jumlah Total Bakteri dan Nilai Organoleptik Ikan Kembung (Rastrelliger sp.) [ The Effect of Red Algae Extract (Kappaphycus alvarezii) against the Total Number of Bacteria and Organoleptic Value of Mackerel (Rastrelliger sp.)]
Authors: Tjahyaningsih Wahju , Hakim Dyo Maliki , Sudarno Sudarno , Abdillah Annur Ahadi
Published: February 2019

Abstract Mackerel (Rastrelliger sp.) is a kind of fish that have high economic value and good nutrition but susceptible to decay. People often to use formaldehyde as a preservative so that the fish does not quickly to decay. The use of formaldehyde could be replaced by natural ingredients that contain antibacterial compounds, one of which is Kappaphycus alvarezii. This study aims to determine the effect of red algae extract (K.alvarezii) against the total number of bacteria and organoleptic value of mackerel (Rastrelliger sp.). The research design that done in this study is Completely Randomized Design (CRD). The treatments that given are immersion of mackerel in a solution of extract of K. alvarezii with a concentration of 0 ppm, 600 ppm, 700 ppm, 800 ppm and 1% solution of formaldehyde with four repetitions in each treatment. Data analyzes using Analysis of Variants (ANOVA) and followed by Duncan's Multiple Range Test to determine differences between treatments. The results showed that the extract of red algae (K.alvarezii) at a concentration of 0 ppm, 600 ppm, 700 ppm and 800 ppm significantly (p <0.05) against the total number of bacteria of mackerel (Rastrelliger sp.). The ability of extract of K. alvarezii at a concentration of 800 ppm equivalent to the ability of formaldehyde as an antibacterial ingredient in mackerel. K.alvarezii extract could inhibit the growth of bacteria but have not been able to maintain the quality of mackerel based on organoleptic test.
Studi Bioakumulasi Timbal (Pb) pada Ikan Bandeng (Chanos chanos Forskall) di Tambak Sekitar Perairan Sungai Buntung, Kabupaten Sidoarjo [ Studies Bioaccumulation of Lead (Pb) in Milkfish (Chanos chanos Forskall) at The Fishponds Around Buntung River, Sidoarjo]
Authors: Triastuti Rr. Juni , Aditama Singgih , Rahardja Boedi Setya
Published: February 2019

Abstract Lead (Pb) has been recognized as hazardous metal because it is commonly found as contaminant, interfere the survival of aquatic organisms, and cause bioaccumulation process in aquatic biota at the waters. Bioaccumulation refers to the accumulation of substances in organism that can trough the food chain reaction of aquatic tissue. Bioaccumulation may occur in milkfish cultivated in fishponds around the Buntung river, Sidoarjo, because there are a lot of factories or industrial activities operating and disposing the waste along the river. The purposes of this study are to measure the lead (Pb) content in milkfish at pond around Buntung river, Sidoarjo, and to determine concentration of water and sediment river, water and sediment of fishponds, klekap, and milkfish meat from fishponds, and also to has exceeded specified maximum limit. Design of this study is descriptive study. The samples were collected from 4 stations that located in Buntung river, Sidoarjo, include water and sediment of river, water and sediment of fishponds, klekap, meats and gills from milkfish. Analyze the lead (Pb) content of water, sediment, and biota used Atomic Absorption Spectrophotometry (AAS). The histopathology slide was made by Haematoxylin-Eosin coloration. Cells of controls are the gill filaments at magnification up to 1000 times. The results showed concentration of lead (Pb) in the river water was about 0,018–0,028 ppm, the sediment of river (2,664–3,072 ppm), the water of fishponds (0,000-0,011 ppm), the sediment of fishponds (2,974-3,220 ppm), klekap (0,176-0,192 ppm), and milkfish meat (0,175-0,190 ppm). The lead concentration of each objects have lower than standardization of quality. The injuries found on the gill filaments of milkfish sample histopathologicaly are cell necrosis, rupture of secondary lamella, and congestion.
Perubahan Hematologi Ikan Mas Komet (Carassius auratus auratus) Akibat Infestasi Argulus japonicus Jantan Dan Argulus japonicus Betina [Hematologic Changes Comet Goldfish (Carassius auratus auratus) as a Result of Infestation Argulus japonicus Males And Argulus japonicus Females]
Authors: Kismiyati Kismiyati , Sulmartiwi Laksmi , Isnawati Norma
Published: February 2019

Abstract Diseases of the ornamental fish farming is mainly caused by ectoparasites. Ectoparasites are one cause of the declining value of the commodity selling ornamental fish in Indonesia. Argulus japonicus acute infestation levels could cause death and economic loss to farmers.The aim of this study was to determine the hematologic changes comet goldfish (Carassius auratus auratus) were infested A. japonicus males and females at different degrees of infestation. Research conducted at the Laboratory of Fisheries and Marine Laboratory of Airlangga University and the University of Airlangga Veterinary clinic and Center for Health Laboratory Surabaya in October 2014. The method used in this study is the experimental method. Data hemoglobin, hematocrit, erythrocytes, leukocytes were analyzed using ANOVA to determine the effect of treatment in the study group. This study used four treatments and five replicates with two groups. The variables were observed in this study were hematologic changes due to infestations of A. japonicus. Scoring is done to determine the hematologic changes comet goldfish.
Isolasi dan Identifikasi Bakteri Kitinolitik yang terdapat pada Cangkang Lobster Air Tawar (Cherax Quadricarinatus) [Isolation and Identification of Chytinolitic Bacteria from The Crayfish (Cherax Quadricarinatus) Shell]
Authors: Suprapto Hari , Sudarno Sudarno , Tito Istikhara Mentari
Published: February 2019

AbstrakIndonesia memiliki potensi perikanan yang sangat tinggi, salah satunya adalah lobster. Ekspor lobster air tawar cenderung meningkat tiap tahun. Total ekspor hasil lobster budidaya mencapai 94.511 ton/tahun. Pangsa pasar lobster air tawar tidak hanya terbatas di dalam negeri saja tetapi juga ke luar negeri. Penelitian ini bertujuan untuk mengetahui adanya bakteri kitinolitik dan juga jenis-jenis bakteri kitinolitik yang terdapat pada cangkang lobster air tawar. Penelitian ini menggunakan Penelitian ini menggunakan metode observasi untuk mengetahui adanya bakteri kitinolitik yang ada pada cangkang lobster air tawar. Data yang digunakan dalam penelitian ini adalah secara deskriptif. Data yang diperoleh dianalisis secara deskriptif yaitu penyajian data tentang morfologi dan karakteristik dari bakteri kitinolitik yang diisolasi dari cangkang lobser air tawar (Cherax quadricarinatus) dan dibandingkan dengan morfologi dan karakteristik bakteri kitinolitik dengan literatur yang sesuai. Hasil penelitian ini adalah diperoleh bahwa bakteri yang diisolasi dari cangkang lobster air tawar (Cherax quadricarinatus) dapat tumbuh dan berkembang pada media uji. Kemudian hasil uji hidrolisis bakteri kitinolitik ditandai dengan adanya zona bening yang dihasilkan dari bakteri tersebut. Dari hasil pengujian tersebut didapatkan jenis – jenis bakteri yaitu Aeromonas sp., Bacillus sp. dan Pseudomonas sp. AbstractIndonesia have highest potential of fishery. One of them is Lobster. It has increasing slightly, approximately 94.511 ton/years. The aims this research to determine the presence of chitinolytic bacteria and identify its species in crayfish shells. This research was performed via observation method to determine the presence of chitinolytic bacteria, which are exist in crayfish (cherax quadricarinatus) shell. The data used in this research is descriptive. The data descriptively analyzed represented the morphology and characteristics of chitinolytic bacteria isolated from crayfish (Cherax quadricarinatus) and comparison the morphology and characteristics of chitinolytic bacteria with appropriate literature. These results obtained that the bacteria isolated from crayfish (Cherax quadricarinatus) shell can grow and develop in the test medium. Then, chitinolytic activity was signed by the formation of clear zone on the test medium. The results obtained several bacterium species including Aeromonas sp., Bacillus sp. and Pseudomonas sp.
Chemical Constituents From the Aerial Roots of Ficus benghalensis L., Leaves of Nyctanthes arbor-tristis L. and Roots of Verbesina encelioides (Cav.) Benth. et Hook. f.
Authors: Shahnaz Sultana  , Mohammed Ali  , Showkat Rassol Mir
Published: February 2019

Ficus benghalensis L. (Moraceae) is a native to tropical Asia. Its aerial roots are styptic and taken to alleviate biliousness, dysentery, liver inflammation, jaundice, spermatorrhoea, syphilis and obstinate vomiting.   Nyctanthes arbor-tristis  L. (Oleaceae)  is distributed in the  eastern  Asia including  India, Nepal, Pakistan, Thailand and  Indonesia. Its  leaves are  used to treat  acidity,  asthma,  bronchitis, cough,  dyspepsia,  fevers, hypertension,  malaria, menstrual cramps, piles,  rheumatism,  sciatica, snake bites , strangury and to  expel intestinal worms. Verbesina encelioides (Cav.) Benth. et Hook. f. (Asteraceae) is a native to southeastern North America and one of the most common weeds in northern India after the rainy season. Its roots are used to cure bladder inflammation and also as a blood purifier.  The air-dried plant parts were exhaustively extracted with methanol individually in a Soxhlet apparatus. The concentrated methanol extracts were adsorbed on silica gel for column and chromatographed over silica gel column separately. The columns were eluted with petroleum ether, chloroform and methanol successively to isolate the phytoconstituents. Phytochemical investigation of the aerial roots of F.  benghalensis afforded  n-tritriacontan-10-one (1),  30-lauryloxy- urs-12-en-3β-olyl butyrate (30-lauryloxy-α-amyrin 3-butyrate, 2) and  urs-12-en-23,6α-olide 3β-olyl palmitate (3-palmityl α-amyrin-23,6α-olide, 3). The leaves of N.  arbor-tristis  furnished two vanillyl glycosidic disters characterized as oleiyl-O-α-D-xylopyranosyl-(2a→1b)-O-α-D-xylopyranosyl-2b-vanillyl-4b-caproate (oleiyl-O- α-D-dixylosyl vanillyl caproate, 4) and oleiyl-O-α-D-arabinopyranosyl-(2a→1b)-O-α-D-arabinopyranosyl--(2b→1c)-O-α-D-arabinopyranosyl--(2c→1d)-O-α-D-arabinopyranosyl-2d-vanillyl-4d-caproate  (oleiyl-O- α-D-tetra-arabinosyl vanillyl  caproate, 5). The roots of V.  encelioides produced  tetracosan-1-oyl   1-tetradecanoate   (lignoceryl   myristate,  6), β-amyrin palmitate  (7), urs-12-en-3β-olyl oleate (β-amyrin oleate,  8) and  β-amyrin stearate   (9). The structures of these phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.
Indian Medicinal Plants For Treatment of Ulcer: Systematic Review
Authors: Pahadiya Anitakumari Rambhai  , S S Sisodia
Published: February 2019

Peptic ulcers are a broad term that includes ulcers of digestive tract in the stomach or the duodenum. The formation of peptic ulcers depends on the presence of acid and peptic activity in gastric juice plus a breakdown in mucosal defenses. Gastric ulcers occur commonly at old age and lower socio-economic class of individuals. At the present time many article gives a general idea of ulcer and its treatment by using synthetic drugs. But due to their limitation and side effects we no longer used synthetic drugs. According to published results, it can be said that medical plants are more affordable and have less side effects compared synthetic drugs, and are more effective in treatment of ulcer. India still depends on medicinal plants and they have played an important role in treatment and prevention of ulcers. This article will be concerned only the pharmacology of that plant which shows the antiulcer effects and among all the plants which one is more potent for anti-ulcer. Further investigations are needed to explore the more potent lead compounds with lesser side effects.
O RELÓGIO DE OURO, DE MACHADO DE ASSIS:
Authors: Celestino Emanuely Monteiro , Vieira Precília Achermann , De Lima Braga Penha Gisela Maria
Published: February 2019

O presente trabalho propõe a leitura do conto O relógio de ouro, de Machado de Assis a alunos do 8º e 9º ano do ensino fundamental com o objetivo principal de apontar caminhos diferenciados de trabalho com o texto literário. Para tanto, utilizamos, como aporte teórico fundamental, o livro AULA (2013), de Roland Barthes e sua concepção de forças libertárias: a Mimeses, a Mathesis e a Semiosis, respectivamente: a representação do real, os diferentes saberes e jogo de palavras estabelecido no/pelo texto literário. Para a abordagem e aprofundamento desse trabalho, foram utilizados, também: Barbosa (1996), Barthes (2013), Cândido (2006), Cosson (2016), Soares (2007), dentre outros.
 
Palavras-chaves: literatura; forças da literatura; leitura literária; sala de aula.
ENSINO DE CONCORDÂNCIA VERBAL DE TERCEIRA PESSOA DO PLURAL POR MEIO DE SUPORTES DIGITAIS NO ENSINO FUNDAMENTAL
Authors: Freire Gilson Costa , Do Nascimento Xella Ohana da Cunha
Published: February 2019

Este artigo descreve uma mediação pedagógica aplicada numa turma de 7º ano do Ensino Fundamental para o trabalho com a concordância verbal de 3ª pessoa do plural, por meio de atividades de produção de textos de diferentes gêneros, distribuídos num contínuo oralidade-letramento (BORTONI-RICARDO, 2004), em suportes digitais. O objetivo foi propiciar o multiletramento dos alunos seja no uso de novas tecnologias aplicadas ao ensino, seja na ampliação de seu repertório linguístico quanto à concordância verbal segundo as normas urbanas de prestígio. Os resultados mostraram um crescimento na aplicação da regra em direção à semicategorização (LABOV, 2003) no decorrer das atividades.
PRODUÇÃO DE TEXTOS: ENSINO DE ESTRATÉGIAS DE REFERENCIAÇÃO POR (RE)CATEGORIZAÇÃO
Authors: Moraes Isabela Dias
Published: February 2019

Este artigo apresenta resultados de uma Proposta de Intervenção no ensino aprendizagem da produção textual, em que almejamos ensinar os alunos do 9º ano do Ensino Fundamental, de uma escola pública, a usar estratégias referenciais de (re)categorização por nominalizações na produção de crônicas narrativas. A pesquisa constituiu-se da análise de dois corpus, incluindo produções de textos dos alunos antes da intervenção e a reescrita dos textos ao final. Baseamo-nos nos pressupostos da pesquisa-ação, com fundamentação teórica na perspectiva da Linguística Textual. Os resultados demonstram que os processos de recategorização são essenciais para a produção de sentido e para a progressão textual.
O ENSINO DOS CONECTORES COM USO DE FORMULÁRIOS DO GOOGLE
Authors: Bonjardim da Silva Carmo Bougleux
Published: February 2019

Neste artigo discute-se o tratamento didático do conectores com uso do Google Forms. Nesse contexto, reflete-se na relação entre o ensino da coesão sequencial (KOCH, 2010), os estudos dos marcadores discursivos (ALOMBA RIBEIRO, 2005; PORTOLÉS, 2001) e a pedagogia dos multiletramentos (LEMKE, 2010; ROJO, 2012). Metodologicamente, realiza-se a abordagem teórica dos conceitos para transposição didática e, em seguida, expõe-se a utilização dos formulários. Com isso, amplia-se as propostas de construção dos materiais-fonte, conforme Mendes (2012), mediante emprego de ferramentas em diferentes suportes midiáticos para diversificação metodológica e ensino da articulação textual na Educação Básica.