bluuebunny/crossref_metadata_2025 huggingface dataset:
Synergistic Benefits: Exploring the Anti-Virulence Effects of Metformin/Vildagliptin Antidiabetic Combination against Pseudomonas aeruginosa via Controlling Quorum Sensing Systems
Authors: Khayat Maan T. , Abbas Hisham A. , Ibrahim Tarek S. , Elbaramawi Samar S. , Khayyat Ahdab N. , Alharbi Majed , Hegazy Wael A. H. , Yehia Fatma Al-zahraa A.
Published: May 2023

The repurposing of drugs is one of the most competent strategies for discovering new antimicrobial agents. Vildagliptin is a dipeptidyl peptidase-4 inhibitor (DPI-4) that is used effectively in combination with metformin to control blood glucose levels in diabetic patients. This study was designed to evaluate the anti-virulence activities of this combination against one of the most clinically important pathogens, Pseudomonas aeruginosa. The current findings show a significant ability of the vildagliptin–metformin combination to diminish biofilm formation, bacterial motility, and the production of virulent extracellular enzymes and pyocyanin pigment. Furthermore, this drug combination significantly increased the susceptibility of P. aeruginosa to oxidative stress, indicating immunity enhancement in the eradication of bacterial cells. In compliance with the in vitro findings, the histopathological photomicrographs of mice showed a considerable protective effect of the metformin–vildagliptin combination against P. aeruginosa, revealing relief of inflammation due to P. aeruginosa-induced pathogenesis. P. aeruginosa mainly employs quorum sensing (QS) systems to control the production of its huge arsenal of virulence factors. The anti-virulence activities of the metformin–vildagliptin combination can be interrupted by the anti-QS activities of both metformin and vildagliptin, as both exhibited a considerable affinity to QS receptors. Additionally, the metformin–vildagliptin combination significantly downregulated the expression of the main three QS-encoding genes in P. aeruginosa. These findings show the significant anti-virulence activities of metformin–vildagliptin at very low concentrations (10, 1.25 mg/mL, respectively) compared to the concentrations (850, 50 mg/mL, respectively) used to control diabetes.
Molecular Pathogenesis of Human Immunodeficiency Virus-Associated Disease of Oropharyngeal Mucosal Epithelium
Authors: Tugizov Sharof M.
Published: May 2023

The oropharyngeal mucosal epithelia have a polarized organization, which is critical for maintaining a highly efficient barrier as well as innate immune functions. In human immunodeficiency virus (HIV)/acquired immune deficiency syndrome (AIDS) disease, the barrier and innate immune functions of the oral mucosa are impaired via a number of mechanisms. The goal of this review was to discuss the molecular mechanisms of HIV/AIDS-associated changes in the oropharyngeal mucosa and their role in promoting HIV transmission and disease pathogenesis, notably the development of opportunistic infections, including human cytomegalovirus, herpes simplex virus, and Epstein-Barr virus. In addition, the significance of adult and newborn/infant oral mucosa in HIV resistance and transmission was analyzed. HIV/AIDS-associated changes in the oropharyngeal mucosal epithelium and their role in promoting human papillomavirus-positive and negative neoplastic malignancy are also discussed.
A Newly Developed Method-Based Xanthine Oxidoreductase Activities in Various Human Liver Diseases
Authors: Sato Ken , Naganuma Atsushi , Nagashima Tamon , Arai Yosuke , Mikami Yuka , Nakajima Yuka , Kanayama Yuki , Murakami Tatsuma , Uehara Sanae , Uehara Daisuke , Yamazaki Yuichi , Murase Takayo , Nakamura Takashi , Uraoka Toshio
Published: May 2023

Studies evaluating xanthine oxidoreductase (XOR) activities in comprehensive liver diseases are scarce, and different etiologies have previously been combined in groups for comparison. To accurately evaluate XOR activities in liver diseases, the plasma XOR activities in etiology-based comprehensive liver diseases were measured using a novel, sensitive, and accurate assay that is a combination of liquid chromatography and triple quadrupole mass spectrometry to detect [13C2, 15N2]uric acid using [13C2, 15N2]xanthine as a substrate. We also mainly evaluated the association between the plasma XOR activities and parameters of liver tests, purine metabolism-associated markers, oxidative stress markers, and an inflammation marker. In total, 329 patients and 32 controls were enrolled in our study. Plasma XOR activities were generally increased in liver diseases, especially in the active phase, such as in patients with hepatitis C virus RNA positivity, those with abnormal alanine transaminase (ALT) levels in autoimmune liver diseases, and uncured hepatocellular carcinoma patients. Plasma XOR activities were numerically highest in patients with acute hepatitis B. Plasma XOR activities were closely correlated with parameters of liver tests, especially serum ALT levels, regardless of etiology and plasma xanthine levels. Our results indicated that plasma XOR activity might reflect the active phase in various liver diseases.
Rapid Prototyping Flexible Capacitive Pressure Sensors Based on Porous Electrodes
Authors: Zhao Tiancong , Zhu Huichao , Zhang Hangyu
Published: May 2023

Flexible pressure sensors are widely applied in tactile perception, fingerprint recognition, medical monitoring, human–machine interfaces, and the Internet of Things. Among them, flexible capacitive pressure sensors have the advantages of low energy consumption, slight signal drift, and high response repeatability. However, current research on flexible capacitive pressure sensors focuses on optimizing the dielectric layer for improved sensitivity and pressure response range. Moreover, complicated and time-consuming fabrication methods are commonly applied to generate microstructure dielectric layers. Here, we propose a rapid and straightforward fabrication approach to prototyping flexible capacitive pressure sensors based on porous electrodes. Laser-induced graphene (LIG) is produced on both sides of the polyimide paper, resulting in paired compressible electrodes with 3D porous structures. When the elastic LIG electrodes are compressed, the effective electrode area, the relative distance between electrodes, and the dielectric property vary accordingly, thereby generating a sensitive pressure sensor in a relatively large working range (0–9.6 kPa). The sensitivity of the sensor is up to 7.71%/kPa−1, and it can detect pressure as small as 10 Pa. The simple and robust structure allows the sensor to produce quick and repeatable responses. Our pressure sensor exhibits broad potential in practical applications in health monitoring, given its outstanding comprehensive performance combined with its simple and quick fabrication method.
A Modified Electrochemical Sensor Based on N,S-Doped Carbon Dots/Carbon Nanotube-Poly(Amidoamine) Dendrimer Hybrids for Imatinib Mesylate Determination
Authors: Saleh Mohammadnia Maryam , Roghani-Mamaqani Hossein , Ghalkhani Masoumeh , Hemmati Salar
Published: May 2023

Imatinib mesylate, an anticancer drug, is prescribed to treat gastrointestinal stromal tumors and chronic myelogenous leukemia. A hybrid nanocomposite of N,S-doped carbon dots/carbon nanotube-poly(amidoamine) dendrimer (N,S-CDs/CNTD) was successfully synthesized and used as a significant modifier to design a new and highly selective electrochemical sensor for the determination of imatinib mesylate. A rigorous study with electrochemical techniques, such as cyclic voltammetry and differential pulse voltammetry, was performed to elucidate the electrocatalytic properties of the as-prepared nanocomposite and the preparation procedure of the modified glassy carbon electrode (GCE). A higher oxidation peak current was generated for the imatinib mesylate on a N,S-CDs/CNTD/GCE surface compared to the GCE and CNTD/GCE. The N,S-CDs/CNTD/GCE showed a linear relationship between the concentration and oxidation peak current of the imatinib mesylate in 0.01–100 μM, with a detection limit of 3 nM. Finally, the imatinib mesylate’s quantification in blood-serum samples was successfully performed. The N,S-CDs/CNTD/GCE’s reproducibility and stability were indeed excellent.
Bayesian RC-Frame Finite Element Model Updating and Damage Estimation Using Nested Sampling with Nonlinear Time History
Authors: Wang Kunyang , Kajita Yukihide , Yang Yaoxin
Published: May 2023

This paper proposes a Bayesian RC-frame finite element model updating (FEMU) and damage state estimation approach using the nonlinear acceleration time history based on nested sampling. Numerical RC-frame finite element model (FEM) parameters are selected through nested sampling, and their probability density is estimated using nonlinear time history. In the first step, we estimate the error standard deviation and select the FEM parameters that are required to be updated by FEMU. In the second step, we estimate the probability density of the selected parameters and realize the FEMU through the resampling method and kernel density estimation (KDE). Additionally, we propose a damage state estimate approach, which is a derivative method of the FEMU sample. The numerical results demonstrate that the proposed approach is reliable for the Bayesian FEMU and damage state estimation using nonlinear time history.
Introduction of a Polyethylene Glycol Linker Improves Uptake of 67Cu-NOTA-Conjugated Lactam-Cyclized Alpha-Melanocyte-Stimulating Hormone Peptide in Melanoma
Authors: Qiao Zheng , Xu Jingli , Fisher Darrell R. , Gonzalez Rene , Miao Yubin
Published: May 2023

The aim of this study was to evaluate the effect of linker on tumor targeting and biodistribution of 67Cu-NOTA-PEG2Nle-CycMSHhex {67Cu-1,4,7-triazacyclononane-1,4,7-triyl-triacetic acid-polyethylene glycol-Nle-c[Asp-His-DPhe-Arg-Trp-Lys]-CONH2} and 67Cu-NOTA-GGNle-CycMSHhex {67Cu-NOTA-GlyGlyNle-CycMSHhex} on melanoma-bearing mice. NOTA-PEG2Nle-CycMSHhex and NOTA-GGNle-CycMSHhex were synthesized and purified by HPLC. The biodistribution of 67Cu-NOTA-PEG2Nle-CycMSHhex and 67Cu-NOTA-GGNle-CycMSHhex was determined in B16/F10 melanoma-bearing C57 mice. The melanoma imaging property of 67Cu-NOTA-PEG2Nle-CycMSHhex was further examined in B16/F10 melanoma-bearing C57 mice. 67Cu-NOTA-PEG2Nle-CycMSHhex exhibited higher tumor uptake than 67Cu-NOTA-GGNle-CycMSHhex at 2, 4, and 24 h post-injection. The tumor uptake of 67Cu-NOTA-PEG2Nle-CycMSHhex was 27.97 ± 1.98, 24.10 ± 1.83, and 9.13 ± 1.66% ID/g at 2, 4, and 24 h post-injection, respectively. Normal organ uptake of 67Cu-NOTA-PEG2Nle-CycMSHhex was lower than 2.6% ID/g at 4 h post-injection, except for kidney uptake. The renal uptake of 67Cu-NOTA-PEG2Nle-CycMSHhex was 6.43 ± 1.31, 2.60 ± 0.79, and 0.90 ± 0.18% ID/g at 2, 4, and 24 h post-injection, respectively. 67Cu-NOTA-PEG2Nle-CycMSHhex showed high tumor to normal organ uptake ratios after 2 h post-injection. The B16/F10 melanoma lesions could be clearly visualized by single photon emission computed tomography (SPECT) using 67Cu-NOTA-PEG2Nle-CycMSHhex as an imaging probe at 4 h post-injection. The favorable tumor targeting and biodistribution properties of 67Cu-NOTA-PEG2Nle-CycMSHhex underscored its potential as an MC1R-targeted therapeutic peptide for melanoma treatment.
Surgical Interest of an Accurate Real-World Prediction of Primary Systemic Therapy Response in HER2 Breast Cancers
Authors: Sánchez-Méndez Jose Ignacio , Horstmann Mónica , Méndez Nieves , Frías Laura , Moreno Elisa , Yébenes Laura , Roca Mᵃ José , Hernández Alicia , Martí Covadonga
Published: May 2023

Human epidermal growth factor receptor 2 (HER2)-enriched breast cancers (BC) present the highest rates of pathological response to primary systemic therapy (PST), but they are also the ones that tend to be larger at diagnosis, with microcalcifications and, often, with axillary involvement. If we do not have a reliable method to predict the degree of response, we may not be able to transfer the benefits of PST to surgery. The post-PST surgery planning is guided by the findings in the magnetic resonance imaging (MRI), whose predictive capacity, although high, is far from optimal. Thus, it seems interesting to find other variables to improve it. A retrospective observational study including women with HER2 BC treated with PST and further surgery was conducted. Information regarding clinical, radiological, and histopathological variables was gathered from a total of 132 patients included. Radiological complete response (rCR) was achieved in 65.9% of the sample, and pathological complete response (pCR), according to Miller and Payne criteria, in 58.3% of cases. A higher Ki67 value, the absence of Hormonal Receptors expression, and an rCR was significantly related to a pCR finding. This information impacts directly in surgery planning, as it permits adjustment of the breast resection volume.
The Potential Effect of General Anesthetics in Cancer Surgery: Meta-Analysis of Postoperative Metastasis and Inflammatory Cytokines
Authors: Li Ru , Mukherjee Mousumi Beto , Jin Zhaosheng , Liu Hengrui , Lin Kevin , Liu Qiuyue , Dilger James P. , Lin Jun
Published: May 2023

Metastasis or recurrence following curative surgery is the main indicator of tumor progress and is the main cause of patient death. For more than three decades, the potential for general anesthesia to affect cancer outcomes has been a subject of concern with considerable research interest. Here, we conducted this systematic review and meta-analysis to summarize the effect of inhalational anesthesia (IHNA) vs. propofol-based total intravenous anesthesia (TIVA) on metastasis and recurrence after cancer surgery from clinical and pre-clinical studies. The relative risk for metastasis/recurrence in TIVA is 0.61 (95% confidence interval (95% CI) 0.46 to 0.82, p = 0.0009) compared to IHNA. Inflammatory cytokines have been implicated in cancer metastasis following cancer surgery, thus we analyzed inflammatory cytokines levels after surgery under IHNA or TIVA. Based on pooled analysis, a lower IL-6 level was noticed in TIVA in comparison to IHNA (standardized mean difference (SMD) = 0.77, 95% CI = 0.097 to 1.44, I2 = 92%, p = 0.02) but not TNF-α or IL-10. Preclinical animal model studies show that inhalational anesthetics increase the risk of breast cancer metastasis compared to propofol. In conclusion, the current evidence suggests intravenous anesthetic propofol is associated with less metastasis/recurrence and lower postoperative IL-6 level over inhaled anesthetics in the oncological surgery. We urge more well-designed clinical and preclinical studies in this field.
Main Routes of Production of High-Value-Added 2,5-Furandincarboxylic Acid Using Heterogeneous Catalytic Systems
Authors: Bueno Ane , Barredo Asier , Viar Nerea , Requies Jesus
Published: May 2023

The production of polymers from lignocellulosic biomass is currently one of the challenges to minimizing dependence on fossil fuels such as oil. The cellulosic fraction of this feedstock can be transformed into simple sugars such as glucose or fructose. These sugars can be further converted into 2,5-furandicarboxylic acid (FDCA), a precursor of polyethylene furanoate (PEF). The dehydration of sugars to 5-hydroxymethylfurfural (HMF), a platform molecule to obtain products of interest, has been extensively studied. In addition, the oxidation of this platform molecule to FDCA has been widely investigated. However, a study of the direct or one-step production of FDCA from sugars is needed. This review provides a general overview of the recent research on the catalytic systems for the direct production of FDCA from sugars. Ideally, a single-stage system should be employed. The investigations carried out in a one-step process are first detailed. Different strategies have been tested, such as the physical separation of two phases, where dehydration and oxidation took place separately. In this case, an efficient transfer of HMF is needed. To avoid HMF transfer limitations, other authors focused on the investigation of the one-pot transformation of HMF without physical separation. The major requirement of these processes is to achieve catalytic systems functional for both dehydration and oxidation reactions. Therefore, other investigations focused on the study of two-step integrated systems are also analyzed in this review.
Elucidating Mass Transport within Nanoporous Au for CO2 Electroreduction
Authors: Yang Wenpeng , Qian Lihua , Zheng Rui , Yang Dapeng , Lu Xiyuan
Published: May 2023

The electrochemical reduction of CO2 to value-added chemicals renewable electricity is a promising and ecofriendly strategy to achieve the national strategic goal of “carbon peak and carbon neutrality” and solve the greenhouse effect. Due to the variety of products in CO2 electroreduction (CO2ER), catalytic selectivity has become a key factor in the design of electrode structure. Herein, a systematic investigation of CO2ER on the nanoporous gold films with different thicknesses prepared by the self-deposition method developed by ourselves. Mass transfer effects are found to play an important role in determining product selectivity and activity. The specific activity for CO evolution (jCO) with exponential declination has more dramatic tendency than the specific activity for hydrogen evolution (jH2) with linear decay with increasing nanoporous gold film thickness. Different from the behaviors within the mesoporous structures in previous studies, the retarded transport of HCO3− ions within the nanoscale pores is more sensitive than that of protons. This phenomenon implies the necessity of considering mass transfer effects in the design of outstanding electrocatalysts for CO2ER as well as for understanding the geometrical infrastructure-performance relationships.
Basket to Purkinje Cell Inhibitory Ephaptic Coupling Is Abolished in Episodic Ataxia Type 1
Authors: Martin Henry G. S. , Kullmann Dimitri M.
Published: May 2023

Dominantly inherited missense mutations of the KCNA1 gene, which encodes the KV1.1 potassium channel subunit, cause Episodic Ataxia type 1 (EA1). Although the cerebellar incoordination is thought to arise from abnormal Purkinje cell output, the underlying functional deficit remains unclear. Here we examine synaptic and non-synaptic inhibition of Purkinje cells by cerebellar basket cells in an adult mouse model of EA1. The synaptic function of basket cell terminals was unaffected, despite their intense enrichment for KV1.1-containing channels. In turn, the phase response curve quantifying the influence of basket cell input on Purkine cell output was maintained. However, ultra-fast non-synaptic ephaptic coupling, which occurs in the cerebellar ‘pinceau’ formation surrounding the axon initial segment of Purkinje cells, was profoundly reduced in EA1 mice in comparison with their wild type littermates. The altered temporal profile of basket cell inhibition of Purkinje cells underlines the importance of Kv1.1 channels for this form of signalling, and may contribute to the clinical phenotype of EA1.
Co-Stimulation of AGEs and LPS Induces Inflammatory Mediators through PLCγ1/JNK/NF-κB Pathway in MC3T3-E1 Cells
Authors: Tanabe Natsuko , Tomita Keiko , Manaka Soichiro , Ichikawa Risa , Takayama Tadahiro , Kawato Takayuki , Ono Misae , Masai Yuma , Utsu Akihisa , Suzuki Naoto , Sato Shuichi
Published: May 2023

Advanced glycation end-products (AGEs) are increased under hyperglycemia in vivo and are associated with the onset of diabetes. According to previous studies, AGEs exacerbate inflammatory diseases. However, the mechanism by which AGEs aggravate osteoblast inflammation remains unknown. Therefore, the aim of this study was to determine the effects of AGEs on the production of inflammatory mediators in MC3T3-E1 cells and the underlying molecular mechanisms. Co-stimulation with AGEs and lipopolysaccharide (LPS) was found to increase the mRNA and protein levels of cyclooxygenase 2 (COX2), interleukin-1α (IL-1α), S100 calcium-binding protein A9 (S100A9), and the production of prostaglandin E2 (PGE2) compared to no stimulation (untreated control) or individual stimulation with LPS or AGEs. In contrast, the phospholipase C (PLC) inhibitor, U73122, inhibited these stimulatory effects. Co-stimulation with AGEs and LPS also increased the nuclear translocation of nuclear factor-kappa B (NF-κB) compared to no stimulation (untreated control) or individual stimulation with LPS or AGE. However, this increase was inhibited by U73122. Co-stimulation with AGEs and LPS-induced phosphorylated phospholipase Cγ1 (p-PLCγ1) and phosphorylated c-Jun N-terminal kinase (p-JNK) expression compared to no stimulation or individual stimulation with LPS or AGEs. U73122 inhibited the effects induced by co-stimulation. siPLCγ1 did not increase the expression of p-JNK and the translocation of NF-κB. Overall, co-stimulation with AGEs and LPS may promote inflammation mediators in MC3T3-E1 cells by activating the nuclear translocation of NF-κB via PLCγ1-JNK activation.
PPSW–SHAP: Towards Interpretable Cell Classification Using Tree-Based SHAP Image Decomposition and Restoration for High-Throughput Bright-Field Imaging
Authors: Goktas Polat , Carbajo Ricardo Simon
Published: May 2023

Advancements in high−throughput microscopy imaging have transformed cell analytics, enabling functionally relevant, rapid, and in−depth bioanalytics with Artificial Intelligence (AI) as a powerful driving force in cell therapy (CT) manufacturing. High−content microscopy screening often suffers from systematic noise, such as uneven illumination or vignetting artifacts, which can result in false−negative findings in AI models. Traditionally, AI models have been expected to learn to deal with these artifacts, but success in an inductive framework depends on sufficient training examples. To address this challenge, we propose a two−fold approach: (1) reducing noise through an image decomposition and restoration technique called the Periodic Plus Smooth Wavelet transform (PPSW) and (2) developing an interpretable machine learning (ML) platform using tree−based Shapley Additive exPlanations (SHAP) to enhance end−user understanding. By correcting artifacts during pre−processing, we lower the inductive learning load on the AI and improve end−user acceptance through a more interpretable heuristic approach to problem solving. Using a dataset of human Mesenchymal Stem Cells (MSCs) cultured under diverse density and media environment conditions, we demonstrate supervised clustering with mean SHAP values, derived from the ‘DFT Modulus’ applied to the decomposition of bright−field images, in the trained tree−based ML model. Our innovative ML framework offers end-to-end interpretability, leading to improved precision in cell characterization during CT manufacturing.
Genetically Engineered Triple MAPT-Mutant Human-Induced Pluripotent Stem Cells (N279K, P301L, and E10+16 Mutations) Exhibit Impairments in Mitochondrial Bioenergetics and Dynamics
Authors: Szabo Leonora , Grimm Amandine , García-León Juan Antonio , Verfaillie Catherine M. , Eckert Anne
Published: May 2023

Pathological abnormalities in the tau protein give rise to a variety of neurodegenerative diseases, conjointly termed tauopathies. Several tau mutations have been identified in the tau-encoding gene MAPT, affecting either the physical properties of tau or resulting in altered tau splicing. At early disease stages, mitochondrial dysfunction was highlighted with mutant tau compromising almost every aspect of mitochondrial function. Additionally, mitochondria have emerged as fundamental regulators of stem cell function. Here, we show that compared to the isogenic wild-type triple MAPT-mutant human-induced pluripotent stem cells, bearing the pathogenic N279K, P301L, and E10+16 mutations, exhibit deficits in mitochondrial bioenergetics and present altered parameters linked to the metabolic regulation of mitochondria. Moreover, we demonstrate that the triple tau mutations disturb the cellular redox homeostasis and modify the mitochondrial network morphology and distribution. This study provides the first characterization of disease-associated tau-mediated mitochondrial impairments in an advanced human cellular tau pathology model at early disease stages, ranging from mitochondrial bioenergetics to dynamics. Consequently, comprehending better the influence of dysfunctional mitochondria on the development and differentiation of stem cells and their contribution to disease progression may thus assist in the potential prevention and treatment of tau-related neurodegeneration.
Aβ Oligomer Toxicity-Reducing Therapy for the Prevention of Alzheimer’s Disease: Importance of the Nrf2 and PPARγ Pathways
Authors: Araki Wataru
Published: May 2023

Recent studies have revealed that soluble amyloid-β oligomers (AβOs) play a pathogenetic role in Alzheimer’s disease (AD). Indeed, AβOs induce neurotoxic and synaptotoxic effects and are also critically involved in neuroinflammation. Oxidative stress appears to be a crucial event underlying these pathological effects of AβOs. From a therapeutic standpoint, new drugs for AD designed to remove AβOs or inhibit the formation of AβOs are currently being developed. However, it is also worth considering strategies for preventing AβO toxicity itself. In particular, small molecules with AβO toxicity-reducing activity have potential as drug candidates. Among such small molecules, those that can enhance Nrf2 and/or PPARγ activity can effectively inhibit AβO toxicity. In this review, I summarize studies on the small molecules that counteract AβO toxicity and are capable of activating Nrf2 and/or PPARγ. I also discuss how these interrelated pathways are involved in the mechanisms by which these small molecules prevent AβO-induced neurotoxicity and neuroinflammation. I propose that AβO toxicity-reducing therapy, designated ATR-T, could be a beneficial, complementary strategy for the prevention and treatment of AD.
Anti-Melanogenic Effects of Fractioned Cynanchum atratum by Regulation of cAMP/MITF Pathway in a UVB-Stimulated Mice Model
Authors: Wang Jing-Hua , Hwang Seung-Ju , Lee Sam-Keun , Choi Yujin , Byun Chang Kyu , Son Chang-Gue
Published: May 2023

Based on traditional pharmacological applications and partial in vitro data, Cynanchum atratum (CA) is proposed to act on skin whitening. However, its functional evaluation and underlying mechanisms have yet to be identified. This study aimed to examine the anti-melanogenesis activity of CA fraction B (CAFB) on UVB-induced skin hyperpigmentation. Forty C57BL/6j mice were exposed to UVB (100 mJ/cm2, five times/week) for eight weeks. After irradiation, CAFB was applied to the left ear once a day for 8 weeks (the right ear served as an internal control). The results showed that CAFB significantly reduced melanin production in the ear skin, as indicated by the gray value and Mexameter melanin index. In addition, CAFB treatment notably decreased melanin production in α-MSH-stimulated B16F10 melanocytes, along with a significant reduction in tyrosinase activity. Cellular cAMP (cyclic adenosine monophosphate), MITF (microphthalmia-associated transcription factor), and tyrosinase-related protein 1 (TRP1) were also noticeably downregulated by CAFB. In conclusion, CAFB is a promising ingredient for treating skin disorders caused by the overproduction of melanin and its underlying mechanisms involving the modulation of tyrosinase, mainly mediated by the regulation of the cAMP cascade and MITF pathway.
Screening the Specific Surface Area for Metal-Organic Frameworks by Cataluminescence
Authors: Li Zenghe , Pei Danning , Tian Rui , Lu Chao
Published: May 2023

Metal-organic frameworks (MOFs) are famous for their large surface area, which is responsible for the dispersed active sites and decent behaviors in gas adsorption, storage, and catalytic reactions. However, it remains a great challenge to acquire a cost-effective and accurate evaluation on the surface area for the MOFs. In this work, we have proposed cataluminescence (CTL) to evaluate the specific surface area for the MOFs, based on the adsorption–desorption and the catalytic reaction of ethanol. Aluminum-based MOFs with large-pore (lp), narrow-pore (np), and medium-pore (mp-130, mp-140, and mp-150 synthesized under 130, 140, and 150 °C) have been prepared. Distinguished CTL signals were acquired from ethanol in the presence of these MOFs: lp > mp-150 > mp-130 > mp-140 > np. Note that the CTL intensities were positively correlated with the specific surface areas of these MOFs acquired by the Brunauer–Emmett–Teller (BET) method. The distinct specific surface area of MOFs determined the capacity to accommodate and activate ethanol, leading to the varied CTL intensity signals. Therefore, the proposed CTL could be utilized for the rapid and accurate evaluation of the specific surface area for MOFs. It is believed that this CTL strategy showed great possibilities in the structural evaluation for various porous materials.
Capillary Refill Time and Serum Lactate as Predictors of Mortality and Postoperative Extracorporeal Membrane Oxygenation Requirement in Congenital Heart Surgery
Authors: Cruz Gustavo , Pedroza Gómez Santiago , Arango Akemi , Guevara Paula A. , González Carlos , Aguirre Jesus , Valencia-Orozco Andrea , Suguimoto Antonio J.
Published: May 2023

Multiple tissue perfusion markers are described to guide therapy in critically ill pediatric patients undergoing congenital heart surgery. Given the advantages of capillary refill time, our goal is to determine its predictive capacity for mortality and postoperative extracorporeal oxygenation requirements in congenital heart surgery and compare it to serum lactate. We conducted a prospective cohort observational study in a single high-complexity university hospital. Serum lactate and capillary refill time were measured at five predetermined time points: preoperative, immediate postoperative, 6, 12, and 24 h after the surgery. Prolonged immediate postoperative, 6 h, and 12 h capillary refill time measurements turned out to be independent risk factors for both outcomes. The capillary refill time area under the curve ranged between 0.70 and 0.80, while the serum lactate resulted between 0.79 and 0.92 for both outcomes. Both tissue perfusion markers resulted in mortality and extracorporeal oxygenation requirement predictors. Given the advantages of capillary refill time over serum lactate, a monitoring strategy including these two perfusion markers should be considered for congenital heart surgeries.
Examination of Upper Extremity Length Discrepancy in Patients with Obstetric Brachial Plexus Paralysis
Authors: Danisman Murat , Emet Abdulsamet , Kocyigit Ismail Aykut , Hassa Ercan , Uzumcugil Akin
Published: May 2023

Since the natural course of obstetric brachial plexus palsy is variable, several problems are encountered. One important question, in considering patients with OBPP under observation in outpatient clinical settings, is whether children will have length discrepancies in their arms. The aim of this study was to determine differences in the length of the affected extremity, in comparison to the opposite upper extremity. As such, 45 patients, aged 6 months to 18 years, with unilateral brachial plexus palsy developed due to obstetric reasons, were included in the study. Affected and healthy side humerus, ulna, radius, 2nd metacarpal and 5th metacarpal lengths were evaluated according to gender, age, side, Narakas classification, primary and secondary surgery. Statistically significant differences were found in the change rates of affected/healthy humerus, radius, 2nd metacarpal and 5th metacarpal lengths according to age (93%, 95%, 92%, 90% and 90%, respectively). Affected/healthy change rates of ulna, radius, 2nd metacarpal and 5th metacarpal lengths were found to differ statistically (p < 0.05) according to the Narakas classification variable (94%, 92%, 95%, 94% and 94%, respectively). There were no statistically significant differences in the ratios of affected/healthy change in the lengths of the humerus, ulna, radius and 5th metacarpal compared to the primary surgery (p > 0.05). The ratios of affected/healthy change in ulna, radius and 5th metacarpal lengths were found to differ statistically (p < 0.05) according to secondary surgeries (93%, 91%, 91% and 92%, respectively). Joint and bone deformities and bone shortening were observed after changes that occurred in the postnatal and growing periods due to obstetric brachial plexus palsy. Every increase in function to be gained in the upper extremity musculature was also potentially able to reduce problems, such as shortness.
Could the Hybridization of the SE/TGfU Pedagogical Models Be an Alternative for Learning Sports and Promoting Health? School Context Study
Authors: López-Lemus Ismael , Del Villar Fernando , Rodríguez-Gutiérrez Amparo , González-Silva Jara , Moreno Alberto
Published: May 2023

The present study aims to analyze the influence of the Sport Education (SE)/Teaching for understanding (TGfU) hybrid unit on enjoyment, perceived competence, intention to be physically active, skill execution, decision making, performance and game involvement. A short-term (12-lesson) pre-test/post-test quasi-experimental design was conducted in two groups: control (technical approach: 70 students; age = 14.43 ± 0.693; n = 32 female) and experimental (hybrid unit SE–TGfU: 67 students; age = 13.91 ± 0.900; n = 30 female). The coding instrument was based on the Game performance Assessment Instrument. The Enjoyment and Perceived Competence Scale and the Measure of Intentionality to be Physically Active questionnaire were also used. The results of pairwise comparisons between the groups showed higher post-test scores for most dependent variables for boys and girls using the hybrid SE/TGfU unit. Lower post-test scores were found in pairwise comparisons for several dependent variables in both boys and girls. The present study showed that the application of hybrid models SE/TGfU could increase and help facilitate students’ game involvement and game performance, enjoyment, perceived competence and intention to be physically active, in both boys and girls. In future studies, it would be necessary to analyze psychological variables in the educational context for a deeper assessment.
Emotion Regulation in Toddlerhood: Regulatory Strategies in Anger and Fear Eliciting Contexts at 24 and 30 Months
Authors: Ponzetti Silvia , Spinelli Maria , Coppola Gabrielle , Lionetti Francesca , D’Urso Giulio , Shah Prachi , Fasolo Mirco , Aureli Tiziana
Published: May 2023

The study investigated the emergence of toddlers’ regulatory strategies in aversive contexts. Forty-two toddlers were observed at 24 and 30 months of age using two paradigms designed to elicit fear and anger. We examined toddlers’ use of regulatory strategies at these two stages of life regarding the frequency of self-versus other-oriented strategies and of reactive versus more controlled behaviors. Results showed that the type and level of control of strategies used in toddlerhood in managing negative emotions depend on emotion (e.g., fear versus anger) and age. Toddlers used self-oriented strategies to regulate fear and other-oriented strategies to regulate anger. To manage fear, when toddlers got older, they increased the use of reactive strategies (i.e., releasing tension) and decreased the use of more purposeful strategies (i.e., dealing with the aversive stimulus). In contrast, to regulate anger, toddlers utilized an intermediate level of control (i.e., drawing the mother’s attention to themselves) and increased the use of this strategy with age. In addition, toddlers were able to select appropriate strategies for different stressors, and they increased with age the ability to adapt the strategies to the environmental conditions. Theoretical and practical implications are discussed.
Genetic Disruption of Cilia-Associated Signaling Pathways in Patients with VACTERL Association
Authors: Ritter Jessica , Lisec Kristina , Klinner Marina , Heinrich Martina , von Schweinitz Dietrich , Kappler Roland , Hubertus Jochen
Published: May 2023

VACTERL association is a rare malformation complex consisting of vertebral defects, anorectal malformation, cardiovascular defects, tracheoesophageal fistulae with esophageal atresia, renal malformation, and limb anomalies. According to current knowledge, VACTERL is based on a multifactorial pathogenesis including genomic alterations. This study aimed to improve the understanding of the genetic mechanisms in the development of VACTERL by investigating the genetic background with a focus on signaling pathways and cilia function. The study was designed as genetic association study. For this, whole-exome sequencing with subsequent functional enrichment analyses was performed for 21 patients with VACTERL or a VACTERL-like phenotype. In addition, whole-exome sequencing was performed for three pairs of parents and Sanger-sequencing was performed for ten pairs of parents. Analysis of the WES-data revealed genetic alteration in the Shh- and Wnt-signaling pathways. Additional performed functional enrichment analysis identified an overrepresentation of the cilia, including 47 affected ciliary genes with clustering in the DNAH gene family and the IFT-complex. The examination of the parents showed that most of the genetic changes were inherited. In summary, this study indicates three genetically determined damage mechanisms for VACTERL with the potential to influence each other, namely Shh- and Wnt-signaling pathway disruption, structural cilia defects and disruption of the ciliary signal transduction.
Impact of Dental Trauma on Orthodontic Parameters—A Systematic Review and Meta-Analysis
Authors: Alam Mohammad Khursheed , Awawdeh Mohammed , Aljhani Ali S. , Alotaib Ghada Serhan , Abutayyem Huda , Alswairki Haytham Jamil , Hajeer Mohammad Younis
Published: May 2023

Background and objectives: Investigation into the impact of dental trauma on the results of orthodontic treatment is crucial because it can have a major influence on patient care. However, there has not yet been a thorough review or meta-analysis of the available data, which is inconsistent and scant. Therefore, the goal of this systematic review and meta-analysis is to investigate the impact of dental trauma on orthodontic parameters. Search methods and criterion of selection: Major online databases were searched (beginning from the year 2011) for relevant articles using a properly defined search strategy. Analysis protocol: Risk of bias (RoB) and the Cochrane risk of bias tool were utilized for the purposes of bias evaluation within the individual studies and within the review, respectively. Results: Out of the six clinical trials selected, a significant impact of trauma was observed in individuals in all but one paper. Gender predilection varied across studies and could not be conclusively determined. The follow-up period ranged from two months to two years in the trials. The odds ratio (OR) 0.38 [0.19, 0.77] and the risk ratio (RR) 0.52 [0.32, 0.85] indicated that both the odds as well as the relative risk of experiencing dental trauma were lower in the group with negligible impact compared to the group with noticeable impact. Conclusion and further implications: The findings show that dental trauma significantly affects orthodontic parameters, with lower risk and likelihood of suffering dental trauma in the group with negligible impact than in the group with noticeable impact. However, given the substantial heterogeneity among the studies, it is advised to exercise caution when extrapolating the findings to all populations. Registration and protocol: Registration in the PROSPERO database was carried out before initiating the investigation [CRD42023407218].
Survival with Good Neurological Outcome despite Prolonged Cardiopulmonary Resuscitation and Extreme Acidosis after Out-of-Hospital Cardiac Arrest Due to Acute Myocardial Infarction: A Case Report and Review of the Literature
Authors: Störmann Sylvère , Busygina Kristina , Hein-Rothweiler Ralph , Steffen Julius , Förderreuther Stefanie , Salein Nora , Angstwurm Matthias W.
Published: May 2023

We report the case of a 49-year-old male who suffered from a myocardial infarction with subsequent cardiac arrest. The emergency medical team began cardiopulmonary resuscitation, including defibrillation of ventricular fibrillation. Although a return of spontaneous circulation was achieved after approximately 30 min of continued efforts, the patient went back into cardiac arrest on the way to the hospital and resuscitation had to be resumed. On admission, the patient was severely acidotic with a pH of 6.67, lactatemia of 19 mmol/L, and pronounced hypercapnia (pCO2 127 mmHg). Despite the poor prognosis, all possible efforts including coronary intervention and therapeutic hypothermia were carried out and the patient made a quick recovery with discharge from the intensive care unit on day 5. Survival of extreme acidosis, such as in this case, is rare. This is the first report of survival with good neurologic outcome in a patient with myocardial infarction, cardiac arrest, and pH of under 6.7 on admission at the clinic.
Efficacy Evaluation of Cu- and Ag-Based Antibacterial Treatments on Polypropylene Fabric and Comparison with Commercial Products
Authors: Gallo Nunzia , Iaconisi Giorgia Natalia , Pollini Mauro , Paladini Federica , Pal Sudipto , Nobile Concetta , Capobianco Loredana , Licciulli Antonio , Buonocore Giovanna Giuliana , Mansi Antonella , Salvatore Luca , Sannino Alessandro
Published: May 2023

Filter masks are disposable devices intended to be worn in order to reduce exposure to potentially harmful foreign agents of 0.1–10.0 microns. However, to perform their function correctly, these devices should be replaced after a few hours of use. Because of this, billions of non-biodegradable face masks are globally discarded every month (3 million/minute). The frequent renewal of masks, together with the strong environmental impact of non-biodegradable plastic-based mask materials, highlights the need to find a solution to this emerging ecological problem. One way to reduce the environmental impact of masks, decrease their turnover, and, at the same time, increase their safety level is to make them able to inhibit pathogen proliferation and vitality by adding antibacterial materials such as silver, copper, zinc, and graphene. Among these, silver and copper are the most widely used. In this study, with the aim of improving commercial devices’ efficacy and eco-sustainability, Ag-based and Cu-based antibacterial treatments were performed and characterized from morphological, compositional, chemical–physical, and microbiological points of view over time and compared with the antibacterial treatments of selected commercial products. The results demonstrated the good distribution of silver and copper particles onto the surface of the masks, along with almost 100% antibacterial capabilities of the coatings against both Gram-positive and Gram-negative bacteria, which were still confirmed even after several washing cycles, thus indicating the good potential of the developed prototypes for mask application.
Structural Fault Diagnosis Based on Static and Dynamic Response Parameters
Authors: Yang Qiuwei , Qin Fengjiang , Peng Xi
Published: May 2023

Structural fault diagnosis is an important subject for ensuring the normal use of structures. More test data will help to improve the accuracy and reliability of structural fault diagnosis. Therefore, a structural fault detection algorithm based on static–dynamic mixed sensitivity analysis is proposed. The vibration parameters used were the vibration modes of some of the nodes in the structure measured by the vibration test system. The static response parameter used was the vertical displacement of the structure under the gravity load measured by the static test system. In particular, the gravity load and the structure were connected rigidly to form a new added-mass system. The vibration mode of the additional-mass system was measured again to obtain more equations for fault evaluation. Based on the static and dynamic measurement data, the failure coefficients of all components in the structure were calculated through the mixed sensitivity of the static displacement and vibration-mode shape. According to the calculated value of the failure coefficient, the failure state of all components in the structure could be finally evaluated. The main innovation of the proposed method was the use of the static load as a part of the new added-mass system to obtain more vibration parameters for the defect diagnosis. The implementation process and effect of this method were verified using a numerical truss structure and an experimental steel beam structure. Moreover, the defect diagnosis results of the proposed hybrid method were compared with those of a pure static algorithm and a pure dynamic algorithm to illustrate the advantages of the hybrid method. The research results showed that this method has the advantages of simple implementation and high diagnosis accuracy. Especially for symmetric structures, the proposed method can successfully avoid the possible missed diagnoses of the pure static algorithm and pure dynamic method. The algorithm provides a simple and feasible method for structural defect identification.
Reconstruction of Meteorological Records by Methods Based on Dimension Reduction of the Predictor Dataset
Authors: Balsa Carlos , Breve Murilo M. , Rodrigues Carlos V. , Rufino José
Published: May 2023

The reconstruction or prediction of meteorological records through the Analog Ensemble (AnEn) method is very efficient when the number of predictor time series is small. Thus, in order to take advantage of the richness and diversity of information contained in a large number of predictors, it is necessary to reduce their dimensions. This study presents methods to accomplish such reduction, allowing the use of a high number of predictor variables. In particular, the techniques of Principal Component Analysis (PCA) and Partial Least Squares (PLS) are used to reduce the dimension of the predictor dataset without loss of essential information. The combination of the AnEn and PLS techniques results in a very efficient hybrid method (PLSAnEn) for reconstructing or forecasting unstable meteorological variables, such as wind speed. This hybrid method is computationally demanding but its performance can be improved via parallelization or the introduction of variants in which all possible analogs are previously clustered. The multivariate linear regression methods used on the new variables resulting from the PCA or PLS techniques also proved to be efficient, especially for the prediction of meteorological variables without local oscillations, such as the pressure.
Pressure-Based Posture Classification Methods and Algorithms: A Systematic Review
Authors: Fonseca Luís , Ribeiro Fernando , Metrôlho José
Published: May 2023

There are many uses for machine learning in everyday life and there is a steady increase in the field of medicine; the use of such technologies facilitates the tiresome work of health professionals by either automating repetitive tasks or making them simpler. Bed-related disorders are a great example where tedious tasks could be facilitated by machine learning algorithms, as suggested by many authors, by providing information on the posture of a particular bedded patient to health professionals. To assess the already existing studies in this field, this study provides a systematic review where the literature is analyzed to find correlations between the various factors involved in the making of such a system and how they perform. The overall findings suggest that there is only a significant relationship between the postures considered for classification and the resulting accuracy, despite some other factors such as the amount of data available providing some differences according to the type of algorithm used, with neural networks needing larger datasets. This study aims to increase awareness in this field and give future researchers information based on previous works’ strengths and limitations while giving some suggestions based on the literature review.