bluuebunny/crossref_metadata_2025 huggingface dataset:
Strategies to prevent postoperative delirium: a comprehensive evaluation of anesthesia selection and drug intervention
Authors: Lan Shaoze , Liang Shuai , Wu Huiwen , Deng Shihao , Sun Kenan , Ye Canming , Yang Liu , Ciren Lunzhu , Li Jun
Published: December 2024

Postoperative delirium (POD) represents a common neurological complication encountered predominantly among the elderly cohort undergoing surgical intervention for hip fractures. This phenomenon, particularly commonplace in geriatric populations with heightened preoperative risk profiles, pronounced comorbidities, and later stages of lifespan, poses complex clinical challenges. The impact of perioperative pharmacological interventions and anesthetic strategies on POD’s emergence cannot be understated, as it may profoundly affect the length of hospital stays, rehabilitation milestones, and the overall mortality hazard. The pharmacotherapeutic landscape for managing POD remains constrained, underscoring the imperative nature of preventive measures. Prudent preoperative risk stratification, meticulous intraoperative neuromonitoring guided by electroencephalographic studies, and a holistic postoperative patient welfare model are cornerstone recommendations in the quest to mitigate POD’s incidence. Nonetheless, an extensive exploration into the influence of anesthetic approaches and perioperative medications on the emergence of POD is yet to be satisfactorily charted. Our investigation endeavors to dissect the nexus between anesthetic modalities, perioperative pharmacological interventions, and POD incident rates among the elderly with hip fractures. This study spotlights pivotal determinants of POD in the wake of hip fracture surgery by evaluating and synthesizing data from peer-reviewed sources that adhere to rigorous inclusion criteria. Preliminary studies have revealed that certain anesthesia protocols and perioperative medications may increase the potential incidence of POD, such as higher depth of anesthesia or benzodiazepine use, and the incidence of POD in specific populations, such as patients with higher age, prior history of psychosis, and lower intraoperative oxygen saturation The findings from this study are instrumental in refining strategic perioperative plans tailored for the elderly recipients of hip fracture surgery, aimed at not only diminishing the incidence but also the gravity of POD. Despite these forward steps, the clinical uncertainty concerning the efficacy and safety of the specific drugs and surgical techniques in question remains. These lingering questions underscore the exigency for more extensive, empirically grounded research to consolidate the learnings of this investigation.
Projecting temperature-related dengue burden in the Philippines under various socioeconomic pathway scenarios
Authors: Seposo Xerxes , Valenzuela Sary , Apostol Geminn Louis C. , Wangkay Keith Alexius , Lao Percival Ethan , Enriquez Anna Beatrice
Published: December 2024

Introduction

As climate change advances, the looming threat of dengue fever, intricately tied to rising temperatures, intensifies, posing a substantial and enduring public health challenge in the Philippines. This study aims to investigate the historical and projected excess dengue disease burden attributable to temperature to help inform climate change policies, and guide resource allocation for strategic climate change and dengue disease interventions.

Methods

The study utilized established temperature-dengue risk functions to estimate the historical dengue burden attributable to increased temperatures. Future projections were derived using Coupled Model Intercomparison Project Phase 6 (CMIP6) Shared Socioeconomic Pathway (SSP) scenarios to estimate the excess dengue burden on a national scale. Current health burden estimates were calculated by charting the attributable fraction per epidemiological week against the exponential risk function.

Results

Projections indicate a substantial increase in temperature-related dengue incidence across all SSP climate scenarios by 2100. Between 2010-2019, 72.1% of reported dengue cases in the Philippines were attributable to temperature, demonstrating that temperature is a significant driver in dengue transmission. The highest attributable fractions were observed between the warm-dry season to early rainy season (Epi Weeks 15–25). Southern, periequatorial areas, particularly those undergoing rapid urbanization, had the highest temperature-related dengue incidence.

Discussion

The findings emphasize the critical interplay between climate change and socioeconomic factors in shaping future dengue risk. By incorporating future climate scenarios and provincial-level projections, this study provides valuable insights for policy planning, early warning systems, and public health programming. Strengthening health infrastructure, promoting sustainable urban development, and implementing effective vector control measures are crucial to mitigating the future dengue burden in the Philippines.
Physical activity and anxiety during the COVID-19 pandemic in Tanzania: insights for public health policy in low-income contexts
Authors: Ndabi Joyce Sifa , Simwanza Alfa , Chukwuorji JohnBosco C. , Tladi Dawn , Muomah Rosemary C. , Nwonyi Sampson K. , Tay Doris Akosua , Joachim Dale , Malete Leapetswe , Adamba Clement , Nyawornota Vida Korleki , Nyanynofio Oscar Nyanyo , Donkor Samuel Kofi , Ocansey Reginald
Published: December 2024

Background

The COVID-19 pandemic heightened anxiety levels globally, disproportionately affecting low-and middle-income countries (LMICs). Physical activity (PA) has shown potential to alleviate mental health challenges, including anxiety. This study explores the relationship between PA and anxiety among Tanzanian adults during the pandemic, examining whether self-reported health status moderates this relationship and identifying demographic variations.

Methods

Data from 213 adults were collected using the International Physical Activity Questionnaire-Short Form (IPAQ-SF) and Generalized Anxiety Disorder-7 (GAD-7) scale. Self-reported health was also reported.

Results

Vigorous physical activity was significantly associated with lower anxiety, while moderate physical activity showed weaker effects. Walking and sedentary behavior were not significantly associated with anxiety. Health status did not moderate these relationships, but subgroup analyses indicated stronger effects of vigorous physical activity among men and younger adults.

Conclusion

These findings highlight the relevance of PA, particularly vigorous and moderate physical activity, in supporting mental health in LMIC contexts such as Tanzania. The findings further underscore the importance of targeted, culturally relevant physical activity interventions in LMICs to mitigate anxiety and enhance mental health resilience.
The role of artificial intelligence in preoperative planning for Total Hip Arthroplasty: a systematic review
Authors: Mozafari Javad Khaje , Moshtaghioon Seyed Ali , Mahdavi Seyed Mani , Ghaznavi Alireza , Behjat Morteza , Yeganeh Ali
Published: December 2024

Background

Total Hip Arthroplasty (THA) is a transformative surgical intervention for hip joint disorders, necessitating meticulous preoperative planning for optimal outcomes. With the emergence of Artificial Intelligence (AI), preoperative planning paradigms have evolved, leveraging AI algorithms for enhanced decision support and imaging analysis. This systematic review aims to comprehensively evaluate the role of AI in THA preoperative planning, synthesizing evidence from studies exploring various AI techniques and their applications.

Methods

A systematic search of PubMed, Scopus, and Web of Science databases was conducted to identify relevant articles. Inclusion criteria encompassed studies focusing on AI in THA preoperative planning, including randomized controlled trials (RCTs), observational studies, and comparative studies.

Results

Six studies from China met the inclusion criteria, collectively analyzing 831 patients. AI-assisted planning demonstrated superior accuracy in estimating prosthesis size and positioning compared to traditional methods. However, limitations such as geographic bias and language constraints were noted.

Conclusion

AI-assisted preoperative planning significantly enhances femoral positioning accuracy, providing superior outcomes compared to traditional methods. This improvement in precision, particularly in the placement of femoral and acetabular components, has been consistently observed across studies, making AI an indispensable tool in improving the overall success of Total Hip Arthroplasty. Despite promising findings, further research is warranted to address limitations and optimize the integration of AI technologies into routine clinical practice.
The experiences of attending an international veteran sporting competition and preparatory camp for Team Ukraine during the Russo-Ukrainian conflict
Authors: Castle Claire L. , Heinze Nikki , Gomes Renata S. M.
Published: December 2024

Introduction

This article offers unique insight into Team Ukraine's experiences of attending an international sporting event for veterans living with disability and injured active-duty personnel (the United States' Department of Defense Warrior Games, “The Games”) and a 5-week preparatory camp in the United Kingdom (UK).

Methods

A survey gathered qualitative data at three time points: during the second and final week of training camp, and the in the two weeks immediately following participation at The Games. Forty-four out of 55 members of Team Ukraine (including veterans, active-duty personnel, support staff, and family members) provided responses in Survey 1, 20 in Survey 2, and 18 in Survey 3. These responses were thematically analysed to explore experiences and motivations for participation.

Results and discussion

Five main themes were identified: Rest and recuperation; The ongoing conflict as a source of motivation; The sporting experience; Veterans in sport; and Relationships. Whilst physically demanding, the camp was an opportunity for respite and emotional processing, and participants reflected on social benefits and improvements to physical health and sporting abilities. Perhaps of greatest significance was the role of the ongoing conflict in their motivations to attend The Games. The conflict brought participants together in their collective goals of representing Ukraine on an international stage, raising awareness of the war, and demonstrating strength through sporting success. Findings highlight the challenges experienced and overcome by Team Ukraine and provides insight into the role that competitive sporting events may play during times of conflict as a means of demonstrating national strength and pride, and meeting individuals' health and well-being needs.
Transforming tennis with artificial intelligence: a bibliometric review
Authors: Sampaio Tatiana , Oliveira João P. , Marinho Daniel A. , Neiva Henrique P. , Morais Jorge E.
Published: December 2024

The aim of this study was to conduct a scoping and bibliometric review of articles using artificial intelligence (AI) in tennis. The analysis covered various aspects of tennis, including performance, health, match results, physiological data, tennis expenditure, and prize amounts. Articles on AI in tennis published until 2024 were retrieved from the Web of Science database. A total of 389 records were screened, and 108 articles were retained for analysis. The analysis identified intermittent gaps in publication output during certain intervals, notably in the years 2007–2008 and 2012–2013. From 2012 onward, there was a clear upward trend in publications and citations, peaking in 2022. The theme was led by China, the United States, and Australia. These countries maintain their status as the top contributors in terms of publications. The analysis of author collaborations revealed multiple clusters, with notable contributions from researchers in China, Australia, Japan, and the United States. This bibliometric review has elucidated the evolution of AI research in tennis, highlighting the countries and authors that have significantly contributed to this field over the years. The prediction model suggests that the number of articles and citations on this topic will continue to increase over the next decade (until 2034).
Sustainable agriculture and responsible use of pesticides: commercial crop cultivators’ knowledge, attitudes, and practice perspectives regarding pesticide use
Authors: Udayanga Samitha , Bellanthudawa B. K. A. , De Zoysa H. L. S.
Published: December 2024

The excessive use of pesticides has been identified as a main barrier to sustainable agriculture in the developing countries, that can lead to several negative consequences, posing a significant threat to the total environment. Therefore, the present study investigated the informedness of smallholder vegetable farmers on responsible use of pesticides, exploring how their knowledge, attitudes and protective practices (KAP) are associated with the safe use of pesticides. A questionnaire survey was conducted with 206 smallholder farmers in the central highlands of Sri Lanka to identify how KAP are associated with responsible use of pesticides. Secondly, two focus group discussion were conducted to gain insights into the farmers’ awareness of the responsible use of pesticides. The analysis reveals an overall deficiency in informedness about responsible pesticide use, primarily stemming from moderate level of knowledge and perceptions related to protective practices. Furthermore, farmers tend to face constraints due to limited access to information on responsible use of pesticides and a heavy reliance on pesticides, believing pesticides to be a primary means of increasing crops. Results also indicate a significant association between knowledge, practice, and informedness, whereas attitudes did not significantly contribute to informedness. Significant disparities in informedness were identified among different demographic groups, with men and younger farmers exhibiting higher levels of awareness. Thus, we suggest that farmers’ informedness can be improved by targeted efforts to enhance their knowledge and correct misconceptions on the use of pesticides.
A systematic review on the application of precision livestock farming technologies to detect lying, rest and sleep behavior in dairy calves
Authors: Pesenti Rossi Gaia , Dalla Costa Emanuela , Barbieri Sara , Minero Michela , Canali Elisabetta
Published: December 2024

Welfare studies are increasingly involving the application of Precision Livestock Farming (PLF) sensors, rather than the use of animal-based indicators directly assessed. PLF technology has the advantage to constantly monitor behavior over a long period of time, thus enabling the assessor to identify changes in animal time budgets in real-time. In calves, lying behavior is essential: new-borns have been reported to spend 70–80% of their daily time lying. Growing up, calves progressively reduce the time spent lying; at 3 months, lying behavior occupies around the 50% of their day. Several studies emphasize how lying behavior can be considered as a potential indicator of positive welfare in ruminants, including calves. The aim of this study was to critically revise scientific literature regarding the application of precision livestock farming technologies to measure lying, rest and sleep behaviors in dairy calves. A systematic literature search based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology was conducted through Scopus and Web of Science databases to retrieve full peer-reviewed papers written in English on different PLF technologies applied to measure lying behavior in dairy calves. Literature search retrieved 731 records. After duplicate removal and the application of inclusion criteria, a total of 16 papers were considered eligible for the evaluation. Different PLF technologies and approaches were reported to be used: triaxial accelerometers, machine learning with accelerometer data, computer vision with video cameras, wearable cameras and real-time locating system. Most of the papers (10 out of 16) reported the use of accelerometers, placed on different parts of body of the animal (hind leg, neck, head, ear). Considering the importance that lying behavior has for maintaining homeostasis and development of calves, the possibility to monitor it constantly and reliably with PLF technology would certainly provide a better understanding of calves’ behavior and positive welfare. However, our findings underline PLF technologies still show some practical limitations. Therefore, we must ensure that the sensors are valid and reliable before applying them in practice to detect changes that can be linked with welfare status of calves.
Morphologic study of patent ductus arteriosus based on computed tomography data in 25 dogs
Authors: Umh Heesung , Youp Kyoung-a , Lee Jeongmin , Seo Daeyun , Lim Seongsoo , Namgoong Beomkwan , Choe Ahreum , Hong Hyeajeong , Lee Nanju , Kim Isong , Yoon Junghee , Choi Jihye , Lee Kichang , Yoon Hakyoung , Kim Min Su
Published: December 2024

Introduction

The objective of this study is to analyze the morphology and measurement dimensions of patent ductus arteriosus (PDA) based on computed tomography images.

Methods

The present study retrospectively evaluated computed tomography angiography data from 25 client-owned dogs diagnosed with PDA. PDA was reconstructed based on the central axis and the minimum diameter, ampulla diameter, angle, ampulla cross-section area, and length values were measured at specific measurement sites. Additionally, the minimum diameter ratio, ampulla diameter ratio, ampulla cross-section diameter, and ampulla cross-section diameter/ampulla diameter ratio values were calculated based on direct measurement values.

Results and discussion

The morphology of PDA was distributed as follows: 48% Type IIA, 20% Type IIB, and 32% Type III. A significant correlation was observed between the minimal diameter sagittal and transverse and the ampulla diameter sagittal and transverse, body weight, and angle (descending aorta to PDA). A significant association was observed between ampulla diameter (in both the sagittal and transverse planes) and body weight. The minimal diameter ratio did not demonstrate a significant correlation with the ampulla diameter, body weight, angle and length. However, the ampulla diameter ratio exhibited a significant correlation with the length of the PDA and the angle (descending aorta to PDA). The minimal diameter ratio displayed results that were more closely approximated by a circle, whereas the ampulla diameter ratio showed results that were relatively oval. The ampulla cross-section diameter values differed by an average of 14% from the previously used reference length, ampulla diameter sagittal.

Conclusion

The computed tomography image demonstrated the distinctive cross-sectional configuration of the PDA, which could potentially facilitate advanced pre-procedural planning or the creation of novel occluding devices in the future.
Organic trace elements enhance growth performance, antioxidant capacity, and gut microbiota in finishing pigs
Authors: Xu Weiwei , Zhou Miao , Yang Zhikang , Zheng Mengli , Chen Qinghua
Published: December 2024

Excessive inorganic trace elements are added to livestock and poultry feed to meet the needs of animals, accompanied by frequent occurrence of excretion and gastrointestinal stress. Replacing inorganic trace elements with organic trace elements provides a promising solution to alleviate these problems. Therefore, this study aimed to assess the impact of replacing all inorganic trace elements (ITMs) in feed on the growth performance, meat quality, serum parameters, trace element metabolism, and gut microbiota of finishing pigs. A total of 128 Duroc × Landrace × Yorkshire growing-finishing pigs (33.88 ± 0.62 kg) were assigned to four treatments in a randomized complete block design. Each treatment was divided into four replicates, each containing eight pigs. The control group received a basal diet containing 100% inorganic trace elements, while the experimental groups were provided with diets where all inorganic trace elements were substituted with 30, 50, and 70% organic trace elements. The experiment spanned 56 days. Results indicated that replacing all ITMs with 30, 50, and 70% OTMs demonstrated no adverse effects on average daily feed intake, average daily gain, feed conversion ratio, eye muscle area, backfat thickness, and relative organ weight of finishing pigs compared to the control group. Furthermore, the replacement led to increased serum immunoglobulin A concentration and Cu-SOD enzyme activity, and decreased serum MDA level, and GSH-Px activity in the liver. Notably, 50 and 70% OTMs increased serum Mn-SOD activity, and 70% OTMs increased serum T-AOC content. Moreover, it significantly decreased the excretion of trace elements in feces without compromising their deposition in the muscle. Additionally, replacing 100% ITMs with 30% OTMs resulted in an improved Shannon index of colonic microbiota in finishing pigs. In conclusion, replacing 100% inorganic trace elements with 30, 50, and 70% organic trace elements exhibited no detrimental effects on the performance of finishing pigs. In conclusion, replacing 100% inorganic trace elements with 70% organic trace elements had certain potential to improve the production performance of finishing pigs. This replacement strategy can enhance meat quality, boost antioxidant capacity, reduce trace element excretion, facilitate trace element absorption and deposition, and enhance gut microbiota homeostasis.
Willing but Unable: Moral Distress and Burnout in Italian Veterinarians Working with Companion and Farm Animals
Authors: Prato-Previde Emanuela , De Mori Barbara , Colombo Nicoletta , Pelosi Annalisa
Published: December 2024

Veterinarians face ethical challenges during their careers, but despite evidence of work-related stress and burnout in veterinarians, moral distress has been poorly investigated. Using an online survey, professional experience, moral distress, and burnout were investigated in 704 Italian veterinarians caring for companion animals, farm animals, or both. The reliability of the moral distress questionnaire was optimal, and Factor analysis identified four factors for moral distress: I. Conflict with owner/farmer; II. Relationships with colleagues/superiors; III. Legal context/unsupportive Professional Institutions; IV. Unshared requests for euthanasia. Veterinarians reported rather low levels of moral distress but high levels of work-related stress/anxiety and a decline in empathy over time, mainly toward owners/farmers. More than 50% of veterinarians reported medium/high levels of burnout, and client-related burnout was significantly higher than animal-related burnout. Levels of work-related stress, moral distress, and burnout were higher in women, and experience exerted a protective effect. Moral distress was positively correlated with emotional exhaustion and depersonalization and, to a lesser extent, lower professional fulfillment. The relatively low level of moral distress that emerged in the study needs further investigation, especially considering the high levels of work-related stress and anxiety reported by veterinarians. Women’s greater sensitivity to moral distress and burnout should not be overlooked given the progressive feminization of the veterinary profession worldwide.
Specialized Feed-Additive Blends of Short- and Medium-Chain Fatty Acids Improve Sow and Pig Performance During Nursery and Post-Weaning Phase
Authors: Villagómez-Estrada Sandra , Melo-Durán Diego , van Kuijk Sandra , Pérez José F. , Solà-Oriol David
Published: December 2024

The present study investigates the impact of supplementing diets with a synergistic blend of short- and medium-chain fatty acids (SCFAs-MCFAs) during the peripartum and lactation phases on early microbial colonization and the subsequent growth performance of newborn pigs. The experiment involved 72 sows and their litters, with a follow-up on 528 weaned pigs. Sows were fed either a control diet or a diet supplemented with SCFAs-MCFAs and the pigs were monitored for their growth performance and microbial populations. Subsequently, selected weaned pigs were allotted to an SCFAs-MCFAs diet according to the maternal diet. Results showed that SCFAs-MCFAs supplementation led to reduced backfat loss in sows and improved pig weight and uniformity at weaning (p < 0.05). Additionally, suckling pigs exhibited significant shifts in gut microbiota, including increased lactic acid bacteria and reduced Streptococcus suis populations (p < 0.05). Although there was no influence of maternal diet on pig growth after weaning, there was a modulation on bacterial populations at 7 and 35 days post-weaning. Pigs fed SCFAs-MCFAs demonstrated improved feed efficiency with notable reductions in E. coli and Streptococcus suis counts. The findings suggest that maternal dietary supplementation with SCFAs-MCFAs can positively influence both sow and pig performance, offering a potential strategy to enhance productivity and health in the commercial swine production.
Ancient DNA Reveals the Earliest Evidence of Sheep Flocks During the Late Fourth and Third Millennia BC in Southern Iberia
Authors: Anaya Gabriel , Garrido Juan Manuel , Riquelme José Antonio , Martínez Rafael Mª. , Membrillo Alberto , Caro José Antonio , Pajuelo Ana , Ruiz Adrián , Martín de la Cruz José C. , Molina Antonio
Published: December 2024

The Spanish Merino is the most significant sheep breed globally due to its economic and cultural importance in human history. It has also had a substantial influence on the development of other Merino and Merino-derived breeds. Historical sources indicate that crossbreeding to produce finer, higher-quality wool was already taking place in the south of the Iberian Peninsula during the Roman era. This evidence suggests that individuals with a racial pattern very similar to that of the modern Merino may have already existed on the peninsula. The presence of the skeletal remains of these animals at various human settlements dated to the late fourth and third millennia BC could provide insights into the genomics of these ancestral sheep. This study analyses ancient DNA extracted from nine skeletal remains from three archaeological sites in Southern Iberia, dated to the third millennium BC. The samples were sequenced and aligned with the ovine genome. The genetic distances observed among the samples indicate a closer relationship between several animals from the Marinaleda (Seville) and Grañena Baja (Jaén) sites. The study of the slaughter/death age profiles identified at La Minilla (La Rambla, Córdoba) suggests an approach centred on meat exploitation, while the data from Marinaleda (Seville) and Grañena Baja (Jaén) indicate the potential exploitation of secondary resources. A review of the composition of these small ruminant herds could provide insights into the type of secondary resource exploitation that may have been prioritised. Our aim is to investigate the presence of distinct production systems, differentiating between those aimed primarily at meat use and those focused on secondary products. This is the first approach to exploring the genetic evidence for sheep livestock related to its productive use during this period and in this geographical area.
Residue Elimination Patterns and Determination of the Withdrawal Times of Seven Antibiotics in Eggs of Taihang Chickens
Authors: Chen Huan , Wu Xiajun , Cui Shasha , Li Yandong , Mu Yingli , Gao Jinduo , Liu Huage , Liu Juxiang
Published: December 2024

The objective of this study was to examine the residue elimination patterns of seven antibiotics in the eggs of Taihang chickens under free-range conditions and develop suitable withdrawal times (WDTs). A total of 240 healthy Taihang chickens, aged 180 days, were randomly divided into eight groups of 30 birds each. The first seven groups were administered oxytetracycline, chlortetracycline, erythromycin, tylosin, tylvalosin, lincomycin, and tiamulin, respectively, in accordance with the maximum dosages and longest durations of treatment recommended by the Veterinary Pharmacopoeia of the People’s Republic of China. Group 8 served as the control group, and the test period continued until no drug residue could be detected. The results demonstrate that the residues of oxytetracycline, chlortetracycline, tylosin, tylvalosin, and tiamulin throughout the test period were below the maximum residue limits (MRLs) outlined in GB 31650-2019, “National food safety standard-Maximum residue limits for veterinary drugs in foods”. The egg yolk was identified as the target tissue for estimating the withdrawal time periods (WDTs) of these seven drugs. It was thus concluded that the recommended withdrawal time for tiamulin should be 0 days. However, the WDTs of oxytetracycline, chlortetracycline, tylosin, tylvalosin, were 2.8, 0.3, 2.4, and 7.4, respectively, when the upper limit of the 95% confidence interval was found to be lower than the MRLs. It was thus determined that the recommended WDTs for oxytetracycline, chlortetracycline, tylosin, and tylvalosin should be 3, 1, 3, and 8 days, respectively. In contrast, erythromycin and lincomycin exhibited concentrations below the permitted MRLs on days 7 and 8, respectively. However, the upper limits of the 95% confidence intervals for erythromycin and lincomycin were found to be below the MRLs when the WDTs were 11 and 8.9, respectively. This suggests that the recommended WDTs for these two antibiotics should be 11 days and 9 days.
Mulberry Branch Extracts Enhance the Antioxidant Capacity of Broiler Breast Muscle by Activating the Nrf2 and Cytochrome P450 Signaling Pathway
Authors: Shi Xiang , Qian Wei , Wei Xinlan , Qin Xiaoqing , Han Jinyan , Su Chao , Bao Lijun
Published: December 2024

Mulberry branch extracts (MBEs) have garnered significant attention as natural feed additives and antioxidants; however, their antioxidant properties in meat post-slaughter and their influence on muscle-related metabolic processes remain largely unexplored. Herein, we evaluated the effects of MBEs on the antioxidant capacity and metabolic processes of breast muscle in yellow-feather broilers by adding 0 g/kg, 1.5 g/kg, 3.0 g/kg, and 4.5 g/kg of MBEs to their diets. The results demonstrate that MBEs enhanced the activity of antioxidant enzymes in muscle tissue. Specifically, a real-time quantitative PCR analysis revealed that MBEs increased the expression of antioxidant enzyme genes in a dose-dependent manner, activated the Nrf2 signaling pathway, and upregulated the expression of the Nrf2 gene and its downstream targets at doses of up to 3.0 g/kg. Furthermore, the results of widely targeted metabolomics indicate that the dietary supplementation of MBEs changed the amino acid profile of the muscle, increasing the levels of amino acids and small peptides that contribute to antioxidant properties while reducing the contents of oxidized lipids and carnitine (C5:1) and partially reducing the content of lysophosphatidylcholine (LPC). Notably, at doses of up to 3 g/kg, the levels of five signature bile acids increased in correlation with the added dose. A KEGG analysis indicated that the differential metabolites were predominantly enriched in the metabolism of xenobiotics by cytochrome P450, suggesting that the function of MBEs may be associated with the expression of P450 enzymes. In summary, this study demonstrates that MBEs are effective, safe, and natural antioxidants, offering a viable solution to mitigating oxidative stress in the yellow-feather broiler farming industry and even in livestock farming.
An Investigation of the Saccharides Profile and Metabolic Gene Expression in Muskrat Scented Glands in Different Secretion Seasons
Authors: Zhou Juntong , Hu Defu , Feng Nuannuan , Liu Shuqiang , Li Junqing
Published: December 2024

The adult male muskrat has a pair of scented glands, which show clear seasonal changes in their developmental status between the secretion season and non-secretion season. During the secretion season, the scented glands are much larger than in the non-secretion season, with the metabolism of glandular cells increasing and a large amount of musk being produced. In this work, the blood, musk, and scented gland tissue were collected from three healthy adult male muskrats during secretion season (September). And the blood and scented gland tissue from another three healthy adult male muskrats during the non-secretion season (November) were also sampled. The saccharides from blood and musk were detected by liquid chromatography–mass spectrometry (LC-MS), indicating the saccharides are concentrated in the scented glands during the secretion season. What is more, transcriptome analysis was employed to investigate the expression patterns of saccharides’ pathways, suggesting some saccharides’ metabolism-related genes undergo significant seasonal changes. Above all, scented gland saccharides’ metabolism displays seasonal differences, and the enhancement in saccharides’ metabolic activity during the secretion phase maintains glandular proliferation and secretion function.
Factors Affecting Milk Productivity, Milk Quality and Dairy Cow Health
Authors: Bednarski Michał , Kupczyński Robert
Published: December 2024

Milk and dairy products are considered important sources of nutrients in human nutrition due to their content of high-quality protein, minerals, vitamins, and energy [...]
Improvements in Blood Profiles of Canines Naturally Infected with Triple Blood Pathogens (Babesia vogeli, Ehrlichia canis, and Anaplasma platys) Subsequent to Doxycycline Monotherapy
Authors: Wongtawan Tuempong , Sontigun Narin , Boonchuay Kanpapat , Chiawwit Phatcharaporn , Wongtawan Oraphan , Hayakijkosol Orachun , Boonhoh Worakan
Published: December 2024

Multiple blood pathogen infections are increasingly found in many areas, particularly in tropical regions. This study aimed to investigate the effectiveness and safety of using doxycycline monotherapy to treat triple blood pathogen infection in sheltered dogs. A total of 375 sheltered dogs were screened for blood pathogen using polymerase chain reaction technique (PCR). There were 34 dogs with triple infection (Babesia vogeli, Ehrlichia canis, and Anaplasma platys), and most dogs displayed anemia and thrombocytopenia. These dogs were treated with doxycycline (10 mg/kg/day) orally for four weeks, and their blood profiles were monitored. Almost all pathogens were undetectable by PCR by day 14 and pathogens were all cleared by day 28. Most blood profiles significantly improved after 14 days. The improvement continued after 28 days. The red blood cell and platelet count (PLT) were increased in similar trends between mild-to-moderate and severe thrombocytopenia groups. The average PLT in the mild-to-moderate thrombocytopenia group raised to normal level by day 14, whereas in the severe group, it was gradually increased to normal level by day 70. We propose using doxycycline monotherapy for 28 days to eliminate all pathogens, and facilitate recovery and welfare in dogs suffering with triple blood pathogen infections.
Synergistic Antioxidant Effects of Molecular Hydrogen and Cold Atmospheric Plasma in Enhancing Mesenchymal Stem Cell Therapy
Authors: Artamonov Mikhail Yu. , Pyatakovich Felix A. , Minenko Inessa A.
Published: December 2024

In regenerative medicine, mesenchymal stem cells (MSCs) have shown their importance and potential in tissue reconstruction and immune system modification. However, such cells’ potential is often diminished by factors such as oxidative stress, immune rejection, and inadequate engraftment. This review highlights the role of molecular hydrogen (H2) and cold atmospheric plasma (CAP) as adjunct therapies to improve the effectiveness of MSC therapy. H2 has strong antioxidative and anti-inflammatory actions as it quenches reactive oxygen species and positively stimulates the Nrf2 pathway that promotes MSC survival and life. CAP, being a modulated source of ROS and RNS, also assists MSCs by altering the cellular redox balance, thus facilitating cellular adaptation, migration, and differentiation. H2 and CAP in conjunction with each other assist in establishing an ambience favorable for promoting MSCs’ survival and growth abilities, and reduce the healing time in various pathways such as wound, neuroprotection, and ischemia. Besides these concerns, this review also covers the best administration routes and doses of H2 and CAP together with MSCs in therapy. This study informs on a novel dual method aimed at improving the outcome of MSC therapy while adding several molecular targets and relevant clinical uses concerning these therapies. Research of the future has to deal with bettering these protocols so that the therapeutic benefits can be maximized without long-term implications for clinical applications.
Research on Transboundary Air Pollution Control and Cooperative Strategies Based on Differential Game
Authors: Yu Chengyue , Tu Guoping , Yu Feilong
Published: December 2024

This paper examines control and cooperation mechanisms for trans-regional air pollution using differential game theory. This study focuses on analyzing pollution control pathways in regions characterized by asymmetric economic development. Three models are constructed: the Nash non-cooperative game, the pollution control cost compensation mechanism, and the collaborative cooperation mechanism. These models are used to investigate optimal pollution control strategies for various regions. The findings indicate that the collaborative cooperation model substantially reduces pollution emissions and enhances overall benefits. Additionally, the pollution control cost compensation mechanism alleviates the burden of pollution control on less developed regions. Numerical analysis confirms the effectiveness of the proposed models and offers theoretical foundations and policy recommendations for regional cooperation in pollution prevention.
Analyzing the Reliability and Cost of the Most Commonly Used Dosimeters for Personal Ultraviolet Radiation Monitoring—A Rapid Review
Authors: Caetano Marco , Gregório João , Paulo Marília Silva
Published: December 2024

To identify the most used dosimeters for monitoring ultraviolet radiation (UVR) and analyze their reliability and cost for individual UV exposure monitoring, this study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. An extensive search of the PubMed, Scopus, and Web of Science databases, covering 2005–2023, was conducted, including examining reference lists of retrieved studies. Of the 1202 records, 52 were eligible for analysis. Three types of dosimeters were identified: photosensitive, photochromic, and electronic dosimeters. Photosensitive dosimeters were utilized for 1236 samples across the studies, while photochromic dosimeters were employed for 360 samples. Electronic dosimeters, with a sample size of 3632, were the most extensively studied. This study highlights the variety of resources available for UVR assessment and the significance of specific dosimeter types in this field. Although few studies have explored the costs associated with dosimeter use, electronic dosimeters are the most cost-effective for radiation monitoring and provide the highest accuracy for measuring UVR exposure. Electronic dosimeters, known for real-time data and high precision, are reliable but costly, being approximately 16.5 times more expensive than photosensitive dosimeters and 160 times more expensive than photochromic dosimeters. Photosensitive dosimeters suit large-scale personal use, and photochromic sensors such as polysulphone dosimeters are also reliable. Additional costs for data analysis software, laboratory equipment, or external analysis services may be incurred, especially for advanced research-grade sensors.
The Impacts of Environmental Assessment and Public Appeal on Air Quality: Evidence from the Chinese Provinces
Authors: Li Zhi , Wang Wen , Zhang Zuo
Published: December 2024

Local governments excessively pursued an economic growth-oriented incentive system while neglecting air pollution control for a long time in China. The impacts of environmental assessment and public appeal could potentially promote environmental governance, thus reducing air pollution. Based on panel data of 30 Chinese provinces from 2003 to 2021, we obtain results using the OLS and GLS methods indicating that environmental assessment and public appeal significantly impact both local environmental governance investments and environmental legislation, with environmental legislation having a more significant effect. Compared to environmental letters and visits, environmental proposals from NPC (National People’s Congress) deputies and CPPCC (Chinese People’s Political Consultative Conference) members, as well as public online environmental concerns, have more significant positive impacts on environmental governance. Environmental governance can indeed reduce air pollution and is also affected by the personal characteristics of the officials. Officials who are in their second term or have transferred from other provinces are more willing to implement environmental governance. Older officials and those with higher education are also inclined towards environmental governance. Compared to provincial governors, the results are more significant for CCP (China’s Communist Party) secretaries. We also further perform a series of robustness tests and find that the effect still exists. The presented results provide valuable insights for the optimization of the roles of environmental assessment and public participation, contributing to reforming the environmental governance system in China.
Development of a New Generalizable, Multivariate, and Physical-Body-Response-Based Extreme Heatwave Index
Authors: Cataldi Marcio , Galves Vitor Luiz Victalino , Sphaier Leandro Alcoforado , Garnés-Morales Ginés , Gallardo Victoria , Párraga Laurel Molina , Montávez Juan Pedro , Jimenez-Guerrero Pedro
Published: December 2024

The primary goal of this study is to introduce the initial phase of developing an impact-based forecasting system for extreme heatwaves, utilizing a novel multivariate index which, at this early stage, already employs a combination of a statistical approach and physical principles related to human body water loss. This system also incorporates a mitigation plan with hydration-focused measures. Since 1990, heatwaves have become increasingly frequent and intense across many regions worldwide, particularly in Europe and Asia. The main health impacts of heatwaves include organ strain and damage, exacerbation of cardiovascular and kidney diseases, and adverse reproductive effects. These consequences are most pronounced in individuals aged 65 and older. Many national meteorological services have established metrics to assess the frequency and severity of heatwaves within their borders. These metrics typically rely on specific threshold values or ranges of near-surface (2 m) air temperature, often derived from historical extreme temperature records. However, to our knowledge, only a few of these metrics consider the persistence of heatwave events, and even fewer account for relative humidity. In response, this study aims to develop a globally applicable normalized index that can be used across various temporal scales and regions. This index incorporates the potential health risks associated with relative humidity, accounts for the duration of extreme heatwave events, and is exponentially sensitive to exposure to extreme heat conditions above critical thresholds of temperature. This novel index could be more suitable/adapted to guide national meteorological services when emitting warnings during extreme heatwave events about the health risks on the population. The index was computed under two scenarios: first, in forecasting heatwave episodes over a specific temporal horizon using the WRF model; second, in evaluating the relationship between the index, mortality data, and maximum temperature anomalies during the 2003 summer heatwave in Spain. Moreover, the study assessed the annual trend of increasing extreme heatwaves in Spain using ERA5 data on a climatic scale. The results show that this index has considerable potential as a decision-support and health risk assessment tool. It demonstrates greater sensitivity to extreme risk episodes compared to linear evaluations of extreme temperatures. Furthermore, its formulation aligns with the physical mechanisms of water loss in the human body, while also factoring in the effects of relative humidity.
High-Resolution Air Temperature Forecasts in Urban Areas: A Meteorological Perspective on Their Added Value
Authors: Oswald Sandro M. , Schneider Stefan , Hahn Claudia , Žuvela-Aloise Maja , Schmederer Polly , Wastl Clemens , Hollosi Brigitta
Published: December 2024

Urban environments experience amplified thermal stress due to the climate change, leading to increased health risks during extreme temperature events. Existing numerical weather prediction systems often lack the spatial resolution required to capture this phenomenon. This study assesses the efficacy of a coupled modeling system, the numerical weather prediction AROME model and the land-surface model SURFace EXternalisée in a stand alone mode (SURFEX-SA), in forecasting air temperatures at high resolutions (2.5km to 100m) across four Austrian cities (Vienna, Linz, Klagenfurt and Innsbruck). The system is updated with the, according to the author’s knowledge, most accurate land use and land cover input to evaluate the added value of incorporating detailed urban environmental representations. The analysis focuses on the years 2019, 2023, and 2024, examining both summer and winter seasons. SURFEX-SA demonstrates improved performance in specific scenarios, particularly during nighttime in rural and suburban areas during the warmer season. By comprehensively analyzing this prediction system with operational and citizen weather stations in a deterministic and probabilistic mode across several time periods and various skill scores, the findings of this study will enable readers to determine whether high-resolution forecasts are necessary in specific use cases.
Emotion Recognition Model of EEG Signals Based on Double Attention Mechanism
Authors: Ma Yahong , Huang Zhentao , Yang Yuyao , Zhang Shanwen , Dong Qi , Wang Rongrong , Hu Liangliang
Published: December 2024

Background: Emotions play a crucial role in people’s lives, profoundly affecting their cognition, decision-making, and interpersonal communication. Emotion recognition based on brain signals has become a significant challenge in the fields of affective computing and human-computer interaction. Methods: Addressing the issue of inaccurate feature extraction and low accuracy of existing deep learning models in emotion recognition, this paper proposes a multi-channel automatic classification model for emotion EEG signals named DACB, which is based on dual attention mechanisms, convolutional neural networks, and bidirectional long short-term memory networks. DACB extracts features in both temporal and spatial dimensions, incorporating not only convolutional neural networks but also SE attention mechanism modules for learning the importance of different channel features, thereby enhancing the network’s performance. DACB also introduces dot product attention mechanisms to learn the importance of spatial and temporal features, effectively improving the model’s accuracy. Results: The accuracy of this method in single-shot validation tests on the SEED-IV and DREAMER (Valence-Arousal-Dominance three-classification) datasets is 99.96% and 87.52%, 90.06%, and 89.05%, respectively. In 10-fold cross-validation tests, the accuracy is 99.73% and 84.26%, 85.40%, and 85.02%, outperforming other models. Conclusions: This demonstrates that the DACB model achieves high accuracy in emotion classification tasks, demonstrating outstanding performance and generalization ability and providing new directions for future research in EEG signal recognition.