bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of remodeling of small diameter kidney arteries in the prognosis of progression of tubulointerstitial fibrosis in patients with chronic glomerulonephritis
Authors: Levitskaya E. S. , Batyushin M. M. , Chistyakov V. A. , Razina A. V. , Dudarev I. V.
Published: June 2019

Aim. To assess the role of remodeling of small diameter kidney arteries in the prognosis of progression of tubulointerstitial fibrosis in patients with chronic glomerulonephritis.

Material and methods. The study involved 97 patients with primary glomerulonephritis. The average age of patients was 37,27±1,2 years. The inclusion criterion was the presence of indications for renal biopsy. Renal biopsy was used to analyze renal tissue, in particular, for determining of tubulointerstitial fibrosis (TIF). Based on TIF sign, the patients were divided in 2 groups. The first group includes patients with TIF, the second — without TIF. Vasometry, a measurement of the renal arteries structures, was performed as part of renal biopsy. Parameters of internal and external diameters, wall thickness, intima, media, intima-media complex, vascular mass were analyzed.

Results. The influence of the size of the inner and outer diameters of the interlobular artery (MA) on the studied risk was established. Probability of TIF developing increases with decreasing of MA inner diameter (χ2-criterion =5,34, p=0,02) and increasing of MA outer diameter (χ2-criterion =7,77, p=0,005). Intimal thickness does not have a statistically significant effect on the risk of TIF developing (p=0,29), whereas media thickness has a high statistical significance in renal tissue remodeling (χ2-criterion =13,71, p=0,0002). A statistically significant effect of an increase in the vascular mass on the risk of TIF was determined (χ2-criterion =6,55, p=0,01).

Conclusion. We established that changes in the structure of small diameter kidney arteries in chronic glomerulonephritis occurs according to a hypertrophic type. Remodeling of small diameter kidney arteries plays significant role on the risk of TIF.
Endoskopik ultrasonografi yapılan olgularda propofole ketamin veya fentanil ilavesinin sedo-analjezi kalitesi üzerine etkileri
Authors: DAŞKAYA Hayrettin , UYSAL Harun , YILMAZ İNAL Ferda , ESEN Asım , KARAASLAN Kazım
Published: June 2019

Amaç:Endoskopik ultrasonografi işlemleri genellikle pron

pozisyonunda ve sedo-analjezi altında yapılmaktadır. Bu prospektif çalışmada

tanısal endoskopik ultrasonografi yapılan hastalarda propofol+ketamin ve

propofol+fentanil kombinasyonları ile yapılan sedo-analjezinin etkinliği, hasta

memnuniyeti ve endoskopist memnuniyeti farklılıklarının incelenmesi

amaçlanmaktadır.Gereç-yöntem: Sedo-analjezi ile endoskopik ultrasonografi işlemi

planlanmış 111 hasta çalışmaya dâhil edildi. Tüm hastalara 1mg/kg Lidokain ve

0,01 mg/kg Atropin yapıldıktan sonra Propofol+ketamin grubuna (Grup K) 1,5

mg.kg-1 Propofol, 1 mg.kg-1 Ketamin, Propofol+fentanil

grubuna (Grup F) da 1,5 mg.kg-1 Propofol, 1 mcg. kg-1

Fentanil ile sedo-analjezi sağlandı. Hastaların intraoperatif dönemde Richmond

Ajitasyon Skoru, kalp atım hızı, sistolik-diyastolik-ortalama kan basıncı,

periferik oksijen saturasyonu total işlem süresi ve bu esnada tüketilen toplam

propofol miktarı kaydedildi. Tüm hastalarda Numerik Rank Skoru, vizüel ağrı

skoru ve Aldrete derlenme skoru kaydedildi. İşlem bittikten sonra endoskopistten

ve hastadan memnuniyetini 0-4 arsında puanlandırması istenip kaydedildi.Bulgular: Her iki grupta demografik veriler ve total operasyon

süreleri benzerdi. Toplamda tüketilen tüketilen Propofol düzeyi grup F’de daha

düşüktü (p<0.001). Grup F’de derlenme ünitesinde kalış süresi daha kısa

gözlendi (p<0.001). Endoskopist memnuniyetinde herhangi bir fark

gözlenmezken, hasta memnuniyetinde anlamlı fark tespit edildi (p:001). Grup K’da

İntraoperatif kalp atım hızı ve tansiyon arteriyalde anlamlı artışlar

gözlenirken, grup F’de bu değerler daha stabil seyr etti. Derlenme ünitesi

giriş kalp atım hızları ve tansiyon değerleri grup K’da daha yüksek gözlendi.Sonuç: Endoskopik girişim yapılan hastaların büyük

çoğunluğunun ileri yaş ve ek hastalık mevcudiyeti bulunmaktadır. Bu hastalarda

anestezi ile yapılan işlemlerde anestejik ajan seçimi önem arz etmektedir. Bu

açıdan bakıldığında endoskopik girişimlerde sedo-analjezi uygulanmalarında fentanil

propofol kombinasyonunun avantajlı olduğunu düşünmekteyiz.
The relation of chronic idiopathic urticaria with Fibromyalgia, sleep disturbance and anxiety
Authors: KOCA Tuba Tülay , NAZİK Hülya , MÜLAYİM Mehmet Kamil , ÖZTÜRK Perihan
Published: June 2019

Aim:

Fibromyalgia syndrome (FMS) and chronic idiopathic urticaria

(CIU) are closely related due to peripheral neurogenic inflammation

(neuropeptide secretion), immune dysfunction, and somatic complaints such as fatigue,

pain, anxiety, and sleep disturbance. This study aimed to reveal the relationship

of CIU with FMS, sleep disturbance and anxiety.Materials

and Methods: A total of 51 patients with CIU aged

18–64 years (36.2±10.4) and 45 sex- and age-matched healthy controls were

included. Urticaria activity score (UAS)

was assessed for the severity of urticaria. 

The 2010 American College of Rheumatology classification criteria were

used for FMS diagnosis. The participants were evaluated with visual analog scale (VAS), fibromyalgia impact

questionnaire (FIQ), Pittsburgh sleep quality index (PSQI), and Beck anxiety inventory

(BAI).Results:

The presence of FMS and body mass index

(BMI) were significantly higher in the urticaria group compared with the control

group. The mean FIQ and UAS of the urticaria group were 47.0 ± 22.1 and 1.3 ±

0.9, respectively.  UAS was positively

correlated with FIQ, PSQI, BAI and VAS (rho=0.411 p=0.004; rho=0.310 p=0.034;

rho=0.419 p=0.004; rho=0.414 p=0.004; respectively).  The presence of FMS was found to be

significantly associated with high BMI (p = 0.04).

Conclusions:

The prevalence of FMS was higher in patients with CIU than control group. Also,

FMS was more severe, general pain and fatigue, sleep disturbance and anxiety

were higher in patients with high urticarial activity. 
Calculation of the grain potential barrier in the poly− and nanocrystalline semiconductors
Authors: Ilin A. S. , Gololobov V. M. , Forsh E. A. , Forsh P. A. , Kashkarov P. K.
Published: June 2019

The distribution of potential and parameters of potential barrier in semiconductor crystallite was calculated numerically. The calculation was carried out in spherical crystallite with evenly distributed donors and surface states. The calculation assumed that the surface charge is screened by both ionized donors and free electrons, the contribution of which cannot be neglected in semiconductors with high concentration of free electrons. The height of potential barrier is shown to nonmonotonically depend on the concentration of donors. The dependence of height of potential barrier on the concentration of donors may be divided into two part. One part of dependence describes the fully depleted crystallite and the second part describes the party depleted crystallite. On the first part the height of potential barrier increases with the donor concentration but on the second part the height of potential barrier decreases. The height of the potential barrier increases with increasing of concentration of surface states. The possibility of existing of potential barriers is estimated in nano− and polycrystalline metal oxide semiconductors used as sensitive layers of gas sensors. It is concluded that if the radius of crystal grains in metal oxide semiconductors does not exceed 10 nm, the explanation of the sensitivity of the sensor to gas by using a commonly barrier model seems unlikely. It is demonstrated that shape of crystallite and the contribution of free electrons to screening of surface charge have to be taken into account to calculation of width of potential barrier.
About nature of the effective surface charge on InAs crystals transformation during anode oxide layer growing
Authors: Artamonov A. V. , Astakhov V. P. , Warlashov I. B. , Gindin P. D. , Evstafieva N. I. , Mitasov P. V. , Miroshnikova I. N.
Published: June 2019

Dynamics of changes in fluorine atoms distribution through grown anodic oxide layer thickness and the effective surface charge on InAs crystals under such layers has been studied. Anodic oxidation was performed in alkaline electrolyte with fluorochemical additive component in galvanostatic mode at anode current densities 0.05 or 0.5 mA·cm−2. The layers thickness in boundes 32—51 nm varied by electrodes final voltage setting in range 15—25 V. The layer thickness and refractive index was measured by ellipsometric method, and distribution of fluorine atoms through thickness — by photoelectron−spectroscopy method, combined with ion etching. At the same time, based on grown layers there were produced MIS structures, and from calculation of theirs capacitance−voltage characteristics are determined effective surface charge and surface states density, corresponding to different layer thicknesses.

Main results are reduced to the facts during layers growing despite of anodizing current density comes their sealing, the profile of fluorine atoms distribution shifts towards InAs, positive effective surface charge gradually decreases from 3.6 · 1011 to 2.0 · 1011 cm−2 at surface states density in (6—7) · 1011 eV·cm−2 range for all cases. Based on comparison of these data and theoretical concepts of MIS structure charge construction, there was made a conclusion about gradual built−in charge distancing from the border with InAs in the process of growing anodic oxide layer, which explains observed effective surface charge decrease during layer thickness increasing. This results indicates that the layer growth rate exceeds the built−in charge displacement rate towards InAs.
Quantum conductance staircase of holes in silicon nanosandwiches
Authors: Bagraev N. T. , Klyachkin L. E. , Malyarenko A. M. , Khromov V. S.
Published: June 2019

The results of studying the quantum conductance staircase of holes in one−dimensional channels obtained by the split−gate method inside silicon nanosandwiches that are the ultra−narrow quantum well confined by the delta barriers heavily doped with boron on the n−type Si (100) surface are reported. Since the silicon quantum wells studied are ultra−narrow (~2 nm) and confined by the delta barriers that consist of the negative−U dipole boron centers, the quantized conductance of one−dimensional channels is observed at relatively high temperatures (T > 77 K). Further, the current−voltage characteristic of the quantum conductance staircase is studied in relation to the kinetic energy of holes and their sheet density in the quantum wells. The results show that the quantum conductance staircase of holes in p−Si quantum wires is caused by independent contributions of the one−dimensional (1D) subbands of the heavy and light holes; these contributions manifest themselves in the study of square−section quantum wires in the doubling of the quantum−step height (G0 = 4e2/h), except for the first step (G0 = 2e2/h) due to the absence of degeneracy of the lower 1D subband. An analysis of the heights of the first and second quantum steps indicates that there is a spontaneous spin polarization of the heavy and light holes, which emphasizes the very important role of exchange interaction in the processes of 1D transport of individual charge carriers. In addition, the field−related inhibition of the quantum conductance staircase is demonstrated in the situation when the energy of the field−induced heating of the carriers become comparable to the energy gap between the 1D subbands. The use of the split−gate method made it possible to detect the effect of a drastic increase in the height of the quantum conductance steps when the kinetic energy of holes is increased; this effect is most profound for quantum wires of finite length, which are not described under conditions of a quantum point contact. In the concluding section of this paper we present the findings for the quantum conductance staircase of holes that is caused by the edge channels in the silicon nanosandwiches prepared within frameworks of the Hall. This longitudinal quantum conductance staircase, Gxx, is revealed by the voltage applied to the Hall contacts, Vxy, to a maximum of 4e2/h. In addition to the standard plateau, 2e2/h, the variations of the Vxy voltage appear to exhibit the fractional forms of the quantum conductance staircase with the plateaus and steps that bring into correlation respectively with the odd and even fractional values.
The correlation between the transthoracic lung ultrasound score (LUS) and the severity of changes in HRCT in patients with interstitial lung diseases v2
Authors: Milena Adina Man , Elena Dantes , Domokos Hancu Bianca , Ioana Bondor Cosmina , Ruscovan Alina , Adriana Parau , Stefania Motoc Nicoleta , Marc Monica
Published: June 2019

Chest high-resolution computed tomography (HRCT) is considered the "gold" standard radiological method in interstitial lung disease(ILD) patients. The objectives of our study were to evaluate the correlation between the transthoracic lung ultrasound (LUS) score (total LUS score and positive chest areas score) and the severity of changes in HRCT simplified scores, in different interstitial disorders, between LUS and symptoms, LUS and pulmonary function impairment. We have evaluated 58 consecutively patients diagnosed with ILD and compared with a non-healthy control group(n=30). TotalLUS score was correlated with HRCT score(r=0.784,p<0.001) and positive chest areas (intercostal spaces with >3B-lines) were correlated with HRCT score (r=0.805,p<0.005). Area under the receiver operating characteristic curve (ROC) for total LUS score using 5 as a HRCT score cut-off was 0.86(p<0.001), respectively for positive chest areas with intercostal space with >3B-lines was 0.88(p<0.001). The sensitivity(Se) was 76.7%, the specificity(Sp) 92.9%.We found a negative good correlation between both ultrasound scores and DLCO, a good correlation with Borg test dyspnea post-effort and significant desaturation. The use of LUS in ILD patients can be a useful, cheap, accessible and free radiation exposure investigation and can play a complementary role in the diagnosis and monitoring of these patients.
Almond Macaroons Recipe v1
Authors: Teytelman Lenny
Published: June 2019

This is one of the EASIEST deserts to make. It takes almost no time, bakes quickly, and has no butter, wheat flour, or egg yolk. It is also infinitely flexible - add Khalua instead of vanilla, make them chocolaty with a quarter cup of cacao, place an almond inside each one, or substitute almond with shredded coconut (partially or completely). This is inspired by Mark Bittman's macaroons recipe. Bittman, Mark. (2008). How to cook everything. 2,000 simple recipes for great food. Hoboken, NJ :J. Wiley
Sample fixation of biopsy tissue for Electron Microscopy (EM) v1
Authors: Riesterer Jessica , Stempinski Erin , Lopez Claudia
Published: June 2019

The most crucial step in the entire electron microscopy workflow is sample fixation. Tissue needs to be preserved in strong fixative as soon as possible to maintain cellular ultrastructure. In a clinical setting, fixation time is critical in order to capture precious human tissue adequately. Of course, priority is given to patient care, and therefore, the tissue sometimes cannot be handled as quickly as needed for optimal ultrastructure preservation. However, we recommend 2 minutes as a “best practice” time to start of preservation.
Immunofluorescence Staining of Sea Urchin Embryos v1
Authors: Booth David
Published: June 2019

In this lab, you will use immunofluroescence stainging to visualize the wondrous cellular transformations that occur throughout sea urchin development. The goals of this module are 1. To learn about marine organisms at Marine Resources and some of their biology 2. Understand the steps of immunofluorescence and its relative advantages 3. Visualize stained embryos and understained the basic tradeoffs of some types of imaging techniques
Helicase-like transcription factor (Hltf) gene-deletion promotes oxidative phosphorylation (OXPHOS) in colorectal tumors of AOM/DSS-treated mice v1
Authors: Ann Helmer Rebecca , Kaur Gurvinder , Ann Smith Lisa , S. Chilton Beverly
Published: June 2019

The helicase-like transcription factor (HLTF) gene – a tumor suppressor in human colorectal cancer (CRC) - is regulated by alternative splicing and promoter hypermethylation. The detection of hypermethylated HLTF DNA in fecal occult blood tests is an indicator of disease recurrence and poor survival. Hltf-deficiency in the ApcMin/+ mouse strain increased the formation of intestinal adenocarcinoma with a high incidence of gross chromosomal instabilities. To investigate Hltf-deletion effects in CRC without cross-breeding into a tumorigenic strain, Hltf-deletion was studied in mice treated with the carcinogen azoxymethane (AOM) and the proinflammatory agent dextran sodium sulfate (DSS). Hltf-deletion resulted in weight loss beginning at treatment week 6, and poor survival (Kaplan-Meier survival plot). Hltf-deletion increased tumor multiplicity compared to controls, and dramatically shifted the topographic distribution of lesions into the rectum. Differential isoform expression analysis of lesions from control mice revealed both the truncated isoform that lacks a DNA-repair domain and the full length isoform capable of DNA damage repair are present (3:1.8 ratio) during adenocarcinoma formation. iPathwayGuide identified 51 dynamically regulated genes of 10,967 total genes with measured expression. Oxidative Phosphorylation (Kegg: 00190), the top biological pathway perturbed by Hltf-deletion, resulted from increased transcription of Atp5e, Cox7c, Uqcr11, Ndufa4 and Ndufb6 genes, concomitant with increased endogenous levels of ATP (p=0.0176). Upregulation of gene expression, as validated with qRT-PCR, was accompanied by a stable mtDNA/nDNA ratio. This is the first study to show Hltf-deletion in an inflammation-associated CRC model elevates mitochondrial bioenergetics. The distal shift in tumorigenesis indicates the detection of hypermethylated HLTF DNA in stool samples might be a prognostic biomarker for distal (left-sided) CRC in patients with inflammatory bowel disease.
DASH Protocol v2.5 v2
Authors: Lyden Amy , Crawford Emily , Quan Jenai , Caldera Saharai , Dynerman David
Published: June 2019

This protocol is for performing Depletion of Abundant Sequences by Hybridization (DASH) after preparing sequencing libraries and pooling together. DASH is most useful for RNA-seq of human metagenomic samples, where abundant species such as human mitochondrial ribosomal RNAs (rRNAs) occupy a majority of the sequencing space available, leaving a minor fraction for regions of interest. DASH treatment is performed after ligation of adapters and unique barcoding of the RNA-seq library. It employs CRISPR-Cas9 complexed to a set of guide RNAs (gRNAs) targeted to the abundant regions to be depleted in a given library. These abundant regions in the library are then cleaved, leaving only the fragments with intact adapters on both ends to be further amplified and sequenced.
PENGARUH PENGGUNAAN ALAT PERMAIANAN EDUKATIF BUSY BOOK TERHADAP PERKEMBANGAN KECERDASAN VISUAL-SPASIAL ANAK
Authors: Annisa R. Maryam Nur , Agustin Mubiar , Eliyawati Cucu
Published: June 2019

The Influence Of Teaching And Learning Using Busy Book Educative Game On Children’s Visual-Spatial Intelligence. Visual-spatial intelligence is strongly linked to the cognitive aspect, because this intelligence hones thinking ability and concentration. Teaching and learning using busy book educative game stimulates children’s cognitive aspect, enabling them to have optimal activities and curiosity. The aim of this research is to describe the influence of busy book educative games on children’s visual-spatial intelligence. This research employed quasi-experimental design, more precisely Nonequivalent Control Group Design by conducting observation to the control and experimental groups before and after treatment. The data were collected through observation method. The sample consisted of 15 and 13 experimental and control groups, respectively. The calculation of independent t-test results in 0.000, where 0.000 < 0.05, so that Ho is rejected and Ha is accepted. Based on this finding, it is concluded that busy book educative game has influence on the visual-spatial intelligence of children in the orange group of TK Negeri Pembina Cimahi. Pengaruh Penggunaan Alat Permaianan Edukatif Busy Book Terhadap Perkembangan Kecerdasan Visual-Spasial Anak. Kecerdasan visual-spasial erat kaitannya dengan aspek kognitif  karena kemampuan yang ada pada kecerdasan visual-spasial ini mengasah kemampuan berfikir dan konsentrasi. Melalui pembelajaran dengan menggunakan Alat Permainan Edukatif busy book ini menstimulasi aspek kognitif anak memungkinkan anak menunjukan aktivitas dan rasa ingin tahu yang optimal. Tujuan dari penelitian ini adalah untuk mendeskripsikan bagaimana pengaruh alat permainan edukatif busy book terhadap kecerdasan visual-spasial anak. Penelitian ini menggunakan desain penelitian Quasi-Experimental jenis Nonequivalent Control Group Design dengan melakukan observasi sebelum perlakuan dan sesudah perlakuan pada kelompok kontrol dan eksperimen. Metode pengumpulan data yang digunakan adalah metode observasi. Sampel pada penelitian ini masing-masing berjumlah 15 anak dari kelompok eksperimen dan 13 anak dari kelompok kontrol. Berdasarkan hasil perhitungan menunjukkan bahwa hasil uji t independen adalah 0,000, yang artinya 0,000 < 0,05, maka Ho ditolak dan Ha diterima. Berdasarkan penelitian tersebut dapat disimpulkan bahwa ada pengaruh Alat Permainan Edukatif (APE) busy book terhadap kecerdasan visual-spasial anak di kelompok jeruk di TK Negeri Pembina Cimahi. Rekomendasi dari penelitian ini adalah guru diharapkan dapat menggunakan alat permainan edukatif busy book untuk menstimulus perkembangan kecerdasan visual-spasial anak.
Overcoming Barriers: The Local and the Innovative Dimensions of Inclusive Socio-Educational Practices
Authors: Antunes Fátima , Lúcio Joana
Published: June 2019

This paper discusses some results of a broader research, focusing on a set of eleven socio-educational practices aiming to overcome school failure and dropout, developed in Portugal, giving particular attention to the local and innovative dimensions. This research aims to understand the point of view of the several actors involved, about which factors, processes and relationships contribute the most to building such practices. Data was gathered through documental analysis and semi-structured interviews with those (institutionally) responsible for each practice under study, and was analysed using two instruments. From the point of view of the people responsible, the practices that contribute the most to overcoming school failure and dropout fall into one of four categories: Study Support (4 Practices), Student Grouping (3), Mediation (3) and Pedagogical Differentiation (1). Some practices mobilise resources; others interfere with learning and life contexts, in order to confront institutional, situational and dispositional barriers to participation and learning. Those practices seem to have an impact on school-family communication. Formal schooling, as well as the socio-cultural inclusion of youth from disadvantaged backgrounds, are seen as relevant; yet, we can observe a somewhat fragile involvement of families and communities in practices aimed at promoting their youth’s educational success.
BRCa1 participates in XIST RNa localization on inactive x chromosome
Authors: Shestakova E. A. , Bogush T. A.
Published: June 2019

Introduction . Inactive X chromosome (Xi) is associated with noncoding XIST RNA, series of proteins and contains multiple epigenetic modifications that altogether determine a silence of the most of X-linked genes. Recently the data were obtained that tumor suppressor BRCA1 is also associated with Xi. The purpose of this study was to reveal the colocalization of BRCA1 and XIST RNA and precise spatial organization on Xi with the high resolution of confocal microscopy.

Materials and methods . The object of the study is IMR90hTERT diploid immortalized fibroblast cell line. For BRCA1 and XIST RNA colocalization analysis on Xi the method of fluorescent hybridization in situ associated with immunofluorescent cell staining (immunoFISH) and confocal microscopy were used. For BRCA1 and heterochromatin protein-1 colocalization study the method of double immunofluorescent staining and common fluorescent microscopy were applied.

Results . The study using confocal fluorescent microscopy with higher resolution has demonstrated at first the colocalization of BRCA1 with XIST RNA region of Xi revealed with XIST RNA probes and with replicating Xi and autosomes revealed with BrdU in late S-phase of cell cycle. Altogether, the data obtained suggest the involvement of BRCA1 in the inhibition of gene expression on Xi due to the regulation of XIST RNA association with Xi. Moreover, according to the results of confocal microscopy, BRCA1 also colocalizes with replicating Xi and autosomes revealed with BrdU in late S-phase of cell cycle. This indicates a possible involvement of this protein in the replication of pericentromeric repeats in cellular chromosomes. Colocalization of BRCA1 with heterochromatin protein-1α presented in pericentromeric regions of all chromosomes supports this suggestion.

Conclusions . Altogether, the data obtained in this study suggest the involvement of BRCA1 in the inhibition of gene expression on Xi due to the association with noncoding inhibiting XIST RNA and in replication of heterochromatin regions.
Study of antitumor activity of synthetic peptide ryqlhpyr on the prostate cancer cells
Authors: Akentieva N. P. , Shushanov S. S.
Published: June 2019

Introduction . The RHAMM (hyaluronan mediated mobility receptor) is overexpressed in many types of human cancer and increased synthesis of the RHAMM usually correlates with a poor prognostic factor. In this paper, we synthesized the peptide-RYQLHPYR modulating the activity of the RHAMM and examined the therapeutic potential of this RHAMM-targeting peptide as an antitumor agent.

Objective . Study the effect of the synthetic peptide RYQLHPYR on viability, apoptosis, necrosis, caspase-3 / 7 activity, and invasion of prostate cancer cells.

Materials and methods . The peptide RYQLHPYR was prepared by solid phase synthesis. Human prostate cancer cells (PC3 m-LN4), murine embryonic fibroblasts and murine embryonic fibroblasts (RHAMM- / -). To quantify the effect of the peptide on apoptosis and cell necrosis, ELISAPLUS was used. The activity of caspase-3 / 7 was determined by the colorimetric method. Evaluation of the anti-metastatic effect of the peptide in vitro was evaluated by invasion of cells by quantitative analysis of the area of degradation of fluorescent gelatin.

Results . It was found that the peptide RYQLHPYR inhibited the growth of tumor cells PC3 m-LN4 at a concentration of 10 μg / ml (2 × 10–7 M) after 24 h by ~80 %. It was shown that the peptide stimulated the level of apoptosis in cancer cells, approximately 10-fold. It was found that the peptide increased the necrotic death of tumor cells by 2.5 times. During the research it was revealed that the peptide increased the caspase-3 / 7 activity in tumor cells by 2 times. At the same time, it was shown that RHAMM-targeting peptide had no significant effect on apoptosis and necrosis of normal cells (fibroblasts) and fibroblasts (RHAMM- / -). It was found that the peptide inhibited invasion of tumor cells by ~99.86 % at a concentration of 10 μg / ml (2 × 10–7 M).

Conclusions . The obtained results indicate that the peptide RYQLHPYR has antitumor activity and, therefore, has a therapeutic potential for the treatment of prostate cancer.
"La Nuestra Fútbol Feminista": experiencias de un juego posible
Authors: Berardi Maria Jose , Russo Corina , Aon Jimena , Dominich Victoria , Royano Emiliano , Lozano Juliana , Santino Monica
Published: June 2019

La Nuestra Fútbol Feminista sostiene su praxis dedicada al fútbol jugado por mujeres en la Villa 31, de la CABA, Buenos Aires, Argentina. Iniciando su práctica en el año 2007, a partir de una demanda de la comunidad local.
Hoje, o futebol de mulheres
Authors: Goellner Silvana Vilodre , Guimarães Gustavo Cerqueira
Published: June 2019

Apresentação da revista FuLiA / UFMG, v. 4, n. 1, jan.-abr., 2019.
Apresentadora, torcedora ou jogadora: Fernanda Gentil, Larissa Riquelme e Marta nas representações das mulheres pelo jornalismo esportivo
Authors: Neves Thalita
Published: June 2019

Jornalistas homens e mulheres têm a mesma capacidade para analisar um jogo de futebol, escrever matérias esportivas e contar histórias interessantes do mundo da bola. Mas, por que é que, na maioria dos casos, são apenas elas quem passam por provações (e provocações) de gênero que (des)qualificam seu trabalho relacionando-o ao fato de serem mulheres ou são submetidas a situações que pejorativamente as subjugam como figuras de mero apelo sexual? Sem a pretensão de responder concretamente à pergunta, este artigo debate sobre questões de gênero no jornalismo esportivo, um campo dotado de raízes que envolvem preconceitos não só de gênero, mas também de raça e orientação sexual, ao mesmo tempo em que se configura como uma área em potencial para a contextualização da sensibilidade e subjetividade jornalísticas. Para promover reflexões e apontar desafios e perspectivas da área, a análise parte da abordagem midiática de três casos envolvendo mulheres no esporte – Fernanda Gentil, Larissa Riquelme e Marta – e suas respectivas representações noticiosas, construídas em sua maioria sob óticas que associam as figuras femininas a papéis secundários no jornalismo de esportes, independentemente da posição ocupada pela mulher em pauta no noticiário: apresentadora, torcedora ou jogadora.
Un análisis de las representaciones mediáticas y las desigualdades estructurales en el fútbol de mujeres en Argentina
Authors: Moreira Verónica , Litke Martín Álvarez
Published: June 2019

Este artículo analiza algunos aspectos de la posición subalterna de las atletas argentinas. El caso de las jugadoras que participan en la liga competitiva de la AFA (Asociación de Fútbol Argentino) expone las dificultades que estas deportistas enfrentan a diario cuando quieren desarrollar su práctica. El artículo estudia la relación conflictiva entre las deportistas, las instituciones deportivas y los medios de comunicación; una relación que pone de manifiesto la desigualdad y el trato discriminatorio hacia las futbolistas en dichos ámbitos. Para esto, por un lado, realizamos un diagnóstico de las representaciones de las futbolistas en el diario Olé, medio de comunicación deportivo online que aún publica ejemplares en versión papel; y, por otro lado, analizamos los datos de la observación realizada durante el trabajo de campo en el Club Atlético Boca Juniors, así como también las entrevistas a sus jugadoras y a representantes de otros equipos de la primera división.
Favoreciendo el cambio emocional positivo hacia las ciencias en la formación inicial del profesorado
Authors: Pipitone Vela Carolina , Guitart Josefa , Agudelo Carlos , García LLadó Àngela
Published: June 2019

En este trabajo identificamos las emociones de los futuros maestros (FM) en relación con la física y la química, además de buscar evidencias de que estas sean modificadas a lo largo del contexto de la asignatura Didáctica de la Materia, la Energía y la Interacción (DMEI). Después de pasar un cuestionario a los FM sobre sus emociones antes y después de la asignatura, los resultados muestran una modificación notable de emociones negativas hacia emociones positivas. Dichos cambios se relacionan con la metodología de trabajo implementada en la asignatura, concretamente con el carácter experimental que tiene. Los resultados también sugieren el reto de favorecer que los FM valoren la importancia que tienen los aspectos emocionales del aprendizaje, así como el hecho de dar sentido a los conocimientos.
Indicadores del proceso de control de la propia comprensión durante la lectura de textos de ciencias
Authors: Calvo Valiente Juan José , Gómez López Ángela , Fernández Rivera Juan José , Sanjosé López Vicente
Published: June 2019

El objetivo de este trabajo fue analizar las acciones de control y regulación de la comprensión de estudiantes de Magisterio durante la lectura de textos de ciencias. Se propuso la tarea de juzgar la comprensibilidad de textos en los que se insertaron incoherencias deliberadas que afectaban la relación entre ideas importantes. En un ordenador, de forma individual y usando el software Read & Answer se registraron las acciones de los sujetos durante realización de la tarea. A partir de modelos teóricos de comprensión de la información, y regulación metacognitiva, se definieron indicadores asociados con los procesos de control y regulación. Estos indicadores discriminaron de forma efectiva entre buen o mal resultado.
The Use of IR Fourier Spectroscopy of Saliva for Rapid Assessment of the Level of Lipid Peroxidation Products
Authors: Bel’skaya L.V. , Sarf E.A.
Published: June 2019

Infrared spectroscopy of saliva is an express and non-invasive method of analysis, applicable for diagnostics of various diseases and for studying metabolic processes and adaptive changes in the body. The goal of this study was to determine possibility of analyzing the products of lipid peroxidation (LPO) by using IR spectroscopy of saliva on the example of oncological diseases. The study involved 203 patients with lung cancer (n = 40), breast cancer (n = 50) and the control group (n = 113). Saliva samples were collected in the morning after overnight fast. The content of LPO products (conjugated dienes, and trienes, Schiff bases, malonic dialdehyde MDA) was determined in all samples and the IR absorption spectra were recorded in the range of 500–4000 cm–1. In the IR spectra, an increase in the intensity of the absorption bands of lipids was observed; it corresponded to an increase in the total lipid content and correlated with the content of MDA, and a decrease in the intensity of vibrations of oxygen-containing groups, which corresponded to a negative correlation with secondary LPO products. Apparently, on the IR spectra, we simultaneously register both primary, intermediate, and final LPO products. Statistically significant regression equations were obtained, allowing to estimate the content of intermediate LPO products - conjugated triene, and Schiff bases. The proposed method allows to monitor LPO processes, as well as to characterize the direction of the equilibrium shift in these processes.
Going the Distance: Understanding the Process of Maintaining a Long-Distance Relationship in Graduate School
Authors: Fletcher Emma
Published: June 2019

Graduate studies can be one of the most stressful periods of an individual’s life, and the added stressor of being at a distance from a significant other (SO) can complicate this issue. The partner is an important source of support and understanding during this process, and they can help alleviate stressors during this transition. Graduate students are typically not the focus of the research, and neither are the positive aspects of engaging in an long-distance relationship (LDR). Thus, this study addresses: What has been the process of maintaining an LDR while entering graduate studies? Participants were selected based on heterogenous and opportunistic sampling, and included four heterosexual, female graduate students whom were engaged in an LDR. Semi-structured interviews were utilized in conjunction with an grounded theory approach to better understand the subjective experiences of initiating, transitioning into, and maintaining an LDR while one partner undertook graduate studies.

Keywords: graduate studies, grounded theory, long-distance relationship, mental health, stress
Discoursing Health Literacies for HIV/AIDS Education
Authors: Oguanobi Hembadoon Iyortyer
Published: June 2019

Health literacy is an important tool for HIV/AIDS education.  It provides a space for students to use local literacy tools such as films, literature, and arts to explore ways of managing the HIV/AIDS virus in communities ravaged by the disease. HIV/AIDS affects the lives of millions of people in many African countries and requires a robust strategy by educators to tackle the epidemic and create safe spaces for students in schools and communities where young people face stigma and discrimination for having the virus, or living with people who have the virus. In this paper, the author discusses how students in some African countries respond to locally manufactured HIV literacy educational tools produced by members of the community. The author makes the case that it is important for schools to incorporate locally manufactured HIV/AIDS health literacy instruction into the curriculum; this would allow young people to engage with health literacies that are resonant of their embodied experiences.

Keywords: Africa, discrimination, health literacies, HIV/AIDS, school programs
Exploring the Stories of Asian-Canadian Women on What It Means to Be Bicultural
Authors: Yeung Betty
Published: June 2019

Individuals who identify with two cultures, or bicultural individuals, often face unique challenges as they attempt to negotiate the demands of their ethnic and dominant cultures. While much of the research focuses on conceptualizing and measuring bicultural identity, studies investigating the lived experiences of being bicultural are scarce. This study explores the stories of four second-generation Asian-Canadian women about their bicultural experiences and what being bicultural means to them. Drawing from narrative inquiry, interviews were conducted to collect stories from participants. The findings of this study indicate that there are multiple definitions of being bicultural and elucidate some of the challenges faced by second-generation Asian-Canadian women associated with their multifaceted identity. The results provide a better understanding of how Asian-Canadian women define and interact with their bicultural identity.

Keywords: biculturality, culture, ethnies, identity, second-generation
Aporías entre Pluralismo y Justicia en la Legalización de la Eutanasia en Colombia
Authors: Ovalle Gómez Constanza Eugenia
Published: June 2019

Este artículo propone reflexionar sobre la dificultad de determinar la reivindicación del derecho a morir con dignidad en la forma de eutanasia. Se discuten los conflictos éticos que surgen al ser defendido este derecho en aquellas personas que son incapaces de brindar su consentimiento de manera directa –por su edad o condición– y lo hacen a través de la figura ética y jurídica del “consentimiento por sustitución”.Se analizan cuatro valores que suelen estar en conflicto en estos casos: entre quienes propenden por extremar las medidas terapéuticas (aplicar una tecnología para postergar la muerte) en oposición a quienes consideran que lo mejor sería suspender dichas medidas (permitir el desenlace natural de la muerte); entre quienes reclaman ser escuchados en calidad de sustitutos (libertad), frente a quienes atribuyen una serie de consecuencias desafortunadas si se respeta esta solicitud y consideran que lo justo sería hacer un control social al respecto (justicia). Las tesis para dar solución a tales conflictos son controvertibles y contradictorias en su raciocinio. Se plantea una aproximación metodológica para resolver esta aporía mediante la comprensión de los valores en conflicto y el intercambio que puede hacerse entre ellos. Con esto en mente, se propone una perspectiva bioética sustentada en capacidades críticas de origen dialéctico.
Mejoramiento Deportivo Postconvencional en el Alto Rendimiento: Bioética y Participación de los Beneficios Económicos
Authors: Caro romero Henry David
Published: June 2019

El objetivo de este artículo, es hacer una aproximación fenomenológica al denominado Mejoramiento Deportivo Postconvencional, encaminado a la prolongación del máximo performance, es decir, hacia la posibilidad de mantener un óptimo desempeño por más tiempo, superando el declive fisiológico natural y coartando de cierta manera el derecho a los atletas en formación, el poder disfrutar de las ventajas que representa pertenecer a esta élite, en términos de honor, status y los crecientes estímulos económicos que ofrece el deporte profesional. Primero se hace una aproximación teórica al Mejoramiento Deportivo Postconvencional, definido como el uso de aditamentos biotecnológicos con capacidad de producir transformaciones corporales para aumentar el desempeño. Luego se indagan los conceptos de límite y riesgo desde una perspectiva psicológica dirigida a la idiosincrasia del deportista, al igual de cómo se originan y sustentan las “preferencias individuales” y su evolución. Más adelante, se hace referencia a la participación de los beneficios, idea original del campo de la investigación biomédica, vinculada al deporte de rendimiento dado que una parte de sus avances se aprovechan allí y por último, se retoma el concepto de justicia, para comprender la relación entre deporte profesional, sus beneficios y la noción de frugalidad.
Situación de la violencia sexual en Colombia, 2012-2016
Authors: Perdomo-Sandoval Luis-Albeiro , Cardona-Gómez Gloria-del-Pilar , Urquijo-Velásquez Lenis-Enrique
Published: June 2019

Objetivo: determinar la prevalencia y las características de la violencia sexual en Colombia durante el periodo 2012-2016. Método: se realizó un estudio cuantitativo y descriptivo de todos los casos de violencia sexual reportados al Sistema de Vigilancia en Salud Pública (Sivigila) del 2012 al 2016. Resultados: en el periodo 2012-2016, se reportaron al Sivigila 73.986 casos de violencia sexual, de los cuales el 74,78% ocurrió en las cabeceras municipales; el 65,91% en las viviendas; el 88% de las víctimas eran mujeres y el 49,92% adolescentes. De los agresores, el 91,90% eran hombres con edad promedio de 30 años y el 50,65% no convivía con la víctima. De las víctimas, el 26,1% recibió profilaxis para el vih/sida y el 17,0% para el virus de la hepatitis b; al 45,42% se le proporcionó atención en salud mental, el 31,84% fue remitido a protección y el 56,99% fue reportado a las autoridades competentes. Conclusión: en los 73.986 casos de violencia sexual se encontró un ascenso de la prevalencia que va del 13,45% en el 2012 al 27,96% en el 2016, siendo las mujeres en los ciclos vitales de adolescencia, infancia y la primera infancia las principales víctimas de violencia sexual de agresores hombres con edad promedio de 30 años
Associations of hypertension Diabetes Smoking History and SNPs with Responses to Aflibercept Treatment for tAMD and PCV
Authors: K Tanaka , K Furuya , R Mori , A Kawamura , M Yuzawa , S Yamagami
Published: June 2019

Purpose: To determine the correlation between therapeutic effects of IVA treatment on typical AMD (tAMD), and polypoidal choroidal vasculopathy (PCV) and the history of hypertension, diabetes mellitus, smoking history and single nucleotide polymorphisms (SNPs).Methods: Prospective, interventional study. Subjects were assigned to 125 untreated patients with exudative AMD (tAMD: 58 patients, PCV: 67 patients, male: 91:34, mean age 73.4 years). Among the tAMD patients, there were 28 bimonthly injections 30 who received pro re nata (PRN) injections after three monthly injections. Among the PCV patients, 33 were treated with bimonthly injections and 34 received PRN injections after three monthly injections. Therapeutic effects were evaluated by best-corrected visual acuity (BCVA), central retinal thickness (CRT), subfoveal choroidal thickness (CCT), and exudative change after 3 months and 1 year from initial treatment, and also the history of hypertension, diabetes mellitus, smoking and five SNPs (rs10490924, rs800292, rs699947, rs1061170, rs13278062).Results: Improvements of BCVA, CRT were observed in all groups at 1 year after initial treatment. The one-yearchange in CRT showed significant improvement in nonsmokers than smokers in tAMD. The one-year change in CRT indicated a significant improvement in non-diabetic patients in PCV. There was more exudation at both 3 months and 1 year who had smoking history in tAMD. With respect to the rs1061170 mutation of tAMD, in the case with TT type, significant residual exudation was noted at both 3 and 12 months.Conclusions: The history of smoking and diabetes could be influence to IVA treatment for AMD.
Antimicrobial Peptides and Cancer: Potential Use of Antimicrobial-Like Peptides in Chemotherapy
Authors: GJ Mizejewski
Published: June 2019

Antimicrobial peptides (AMPs) constitute host defense peptides found among insects, fish, amphibians, and mammals including man. The targets of AMPs are gram-negative and gram-positive bacteria, fungi, enveloped viruses, and transformed/cancerous cells. The AMPs are broad spectrum antibiotics which display the propensity to serve as therapeutic agents not only in infectious disease, but also in human cancer. AMPs demonstrate unique properties which include cell membrane penetration, destabilization of biological membranes, ability to form and/or interact with membrane channels, and the capability to modulate host immune responses. The three types of AMPs consists a) naturally-occurring; b) artificially synthesized; and c) cleaved peptide fragments from blood and extracellular matrix proteins. The present treatise presents one such example of an AMP-like peptide derived from a naturally-occurring human protein as a potential candidate for future cancer therapy. The biological activities of human AMP-like peptides as cancer therapeutic agents are reviewed and reported in multiple in vitro and in vivo cancer assays. The possibility of using such human protein-derived peptides as primary and adjunct cancer therapeutic agents is addressed.
Maxillary Ridge Vertical Augmentation Based on a Mixture of Xenogenic and Allogenic Bone Supported by Resorbable Membrane: One Case Report
Authors: M Gonzalez-Menendez , J Gonzalez-Tuñon , S Ordoñez , L Junquera , JA Vega
Published: June 2019

One 60 years-old patient was scheduled for left posterior maxillary ridge augmentation due to failure of standard implants, followed by successful maxillary dental implants. We used an unproved technique consisting in a mixture of 50% allogenic and 50% xenogenic bone supported by reabsorbable membrane. No complications were found at the different stages of the treatment, and at the ending the patient showed a good level of satisfactory outcomes. Radiological evaluation demonstrated ridge augmentation able to support implant, and within the graft islands of connective and bone-like tissue was found. Within these tissues osteoclasts and osteoblast putative cells were found. Results demonstrate that the used method in addition to support implants has osteogenic and bone remodeling activity.
Multidisciplinary Approach in a Child with Complex Trauma of Maxillary Permanent Central Incisors
Authors: MP Lucisano , RGL Corrêa Leite , P Nelson-Filho , AMP Minto , JAB Ferraz , RT Cesco , FK De Carvalho , AM Queiroz
Published: June 2019

The present case report describes a multidisciplinary approach involving endodontic, restorative, surgical and periodontal treatment and rehabilitation of a central incisor with crown-root fracture (CRF) in an 11-year-old child. Although in the future possible sequelae and complications should be considered since it is an extensive trauma, the prognosis of the present case is favorable, with the occurrence of repair, evidenced by clinical, radiographic and tomographic success, after one year and six months of follow-up. It was concluded that the association of prolonged flexible splint, biological endodontic treatment, gingivoplasty and restorative/rehabilitative procedures seem to be an adequate and conservative management for CRF cases.