bluuebunny/crossref_metadata_2025 huggingface dataset:
Exogenous Brassinolide Ameliorates the Adverse Effects of Gamma Radiation Stress and Increases the Survival Rate of Rice Seedlings by Modulating Antioxidant Metabolism
Authors: Lu Yanting , Wang Bingkui , Zhang Mengchao , Yang Wenxin , Wu Mingming , Ye Jing , Ye Shenghai , Zhu Guofu
Published: October 2024

Gamma irradiation-based mutant creation is one of the most important methods for rice plant mutagenesis breeding and molecular biology research. Although median lethal dose irradiation severely damages rice seedlings, applying brassinolide (BR) can increase the survival rate of irradiated seedlings. In this study, we investigated the effects of soaking seeds in solutions containing different BR concentrations (0.001, 0.01, 0.1, 1.0, and 5.0 μmol/L) and then spraying the resulting seedlings twice with 0.1 μmol/L BR. The combined BR treatments markedly decreased the superoxide anion (O2•−), hydrogen peroxide (H2O2), and malondialdehyde contents but increased the chlorophyll content. An appropriate BR treatment of gamma-irradiated samples substantially increased the activities of the antioxidant enzymes superoxide dismutase, peroxidase, and ascorbate peroxidase as well as the proline, ascorbic acid, and glutathione contents in rice seedling shoots. The BR treatment also promoted the growth of seedlings derived from irradiated seeds and increased the shoot and root fresh and dry weights. Most notably, soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BR significantly increased the final seedling survival rate and decreased mutant loss. The study results suggest that exogenous BR treatments can protect rice seedlings from gamma irradiation stress by enhancing antioxidant metabolism.
Systemic Inflammation Across Metabolic Obesity Phenotypes: A Cross-Sectional Study of Korean Adults Using High-Sensitivity C-Reactive Protein as a Biomarker
Authors: Baek Seong-Uk , Yoon Jin-Ha
Published: October 2024

Chronic systemic inflammation is a hallmark of obesity. This cross-sectional study aimed to investigate the association between metabolic obesity phenotypes and inflammatory markers in Korean adults (N = 21,112; mean age: 50.9 ± 16.6). Metabolic obesity phenotypes were categorized into metabolically healthy non-obesity (MHNO), metabolically unhealthy non-obesity (MUNO), metabolically healthy obesity (MHO), and metabolically unhealthy obesity (MUO) based on body mass index and the presence of any metabolic abnormalities. High-sensitivity C-reactive protein (hs-CRP) levels were measured. Multiple linear regression was used to determine the association between obesity phenotypes and hs-CRP levels. In the male sample, compared to the MHNO type, the MUNO, MHO, and MUO types were associated with a 22.3% (95% confidence interval; CI: 14.7–30.3%), 15.8% (95% CI: 2.6–30.7%), and 12.5% (95% CI: 3.0–22.9%) increase in the hs-CRP levels, respectively. The association between metabolic obesity types and hs-CRP levels was stronger among the female sample; compared to the MHNO type, the MUNO, MHO, and MUO types were associated with a 30.2% (95% CI: 22.8–38.2%), 16.0% (95% CI: 6.5–26.4%), and 22.8% (95% CI: 13.6–32.8%) increase in the hs-CRP levels, respectively. Our findings indicate a varying profile of systemic inflammation across different metabolic obesity phenotypes.
Two Regions with Different Expression of Lipogenic Enzymes in Rats’ Posterior Subcutaneous Fat Depot
Authors: Turyn Jacek , Stelmanska Ewa , Szrok-Jurga Sylwia
Published: October 2024

Lipid metabolism in various adipose tissue depots can differ vastly. This also applies to lipogenesis, the process of synthesizing fatty acids from acetyl-CoA. This study compared the expression of some lipogenic enzymes: fatty acid synthase (FASN), ATP-citrate lyase (ACLY), and malic enzyme 1 (ME1) in different regions of the posterior subcutaneous adipose tissue in rats. Methods and Results: Posterior subcutaneous adipose tissue collected from twelve-month-old Wistar rats was divided into six parts (A–F). The expression of genes encoding lipogenic enzymes was assessed by measuring their activity and mRNA levels using real-time PCR. In the gluteal region of the fat pad, there were much higher levels of activity and mRNA for these lipogenic enzymes compared to the dorsolumbar region. The mRNA level of FASN increased by more than twentyfold, whereas the level of ME1 and ACLY increased eight- and fivefold respectively. This phenomenon was observed in both old and young animals. Furthermore, the lack of uncoupling protein one (Ucp1) expression suggests that neither the presence of brown adipocytes in the gluteal part nor the transformation of white adipocytes into beige contributed to the observed differences. Conclusion: These results indicate that the gluteal white adipose tissue appears to be a unique and separate subcutaneous fat depot.
The Yeast Ribosomal Protein Rpl1b Is Not Required for Respiration
Authors: Futcher Bruce
Published: October 2024

Previously, Segev and Gerst found that mutants in any of the four ribosomal protein genes rpl1b, rpl2b, rps11a, or rps26b had a petite phenotype—i.e., the mutants were deficient in respiration. Strikingly, mutants of their paralogs rpl1a, rpl2a, rps11b, and rps26a were grande—i.e., competent for respiration. It is remarkable that these paralogs should have opposite phenotypes, because three of the paralog pairs (Rpl1a/Rpl1b, Rpl2a/Rpl2b, Rps11a/Rps11b) are 100% identical to each other in terms of their amino acid sequences, while Rps26a and Rps26b differ in 2 amino acids out of 119. However, while attempting to use this paralog-specific petite phenotype in an unrelated experiment, I found that the rpl1b, rpl2b, rps11a, and rps26b deletion mutants are competent for respiration, contrary to the findings of Segev and Gerst.
Correction: Ferroni et al. A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue. Int. J. Mol. Sci. 2015, 16, 4666–4681
Authors: Ferroni Letizia , Gardin Chiara , Sivolella Stefano , Brunello Giulia , Berengo Mario , Piattelli Adriano , Bressan Eriberto , Zavan Barbara
Published: October 2024

In the original publication [...]
The Glutamine Synthetases Are Required for Sensory Hair Cell Formation and Auditory Function in Zebrafish
Authors: Zhao Yuanrong , Wang Ziyang , Xu Mengting , Qian Fuping , Wei Guanyun , Liu Dong
Published: October 2024

The development of sensory hair cells (HCs) is closely linked to hearing loss. There are still many unidentified genes that may play a crucial role in HC development and function. Glutamine synthetase, Glul, is expressed in sensory hair cells and auditory organs. However, the role of the Glul gene family in the auditory system remains largely unexplored. This study aims to investigate the function of the Glul gene family in the auditory system. The expression patterns of the glul gene family were examined via in situ hybridization in zebrafish embryos. It was revealed that the expression of glula occurred in the otic vesicle, while glulb was expressed in the neuromast. In contrast, glulc did not exhibit any discernible signal. glula loss of function caused abnormal otolith formation and reduced hair cell number in otic vesicles, while glulb knockdown caused a decrease in HC number in both neuromasts and otic vesicles and impaired auditory function. Furthermore, we found that the knockdown of glulb induces apoptosis of hair cells. Transcriptomic analysis of zebrafish with glula and glulb knockdown revealed significant alterations in the expression of many genes associated with auditory organs. The current study sheds light on the requirement of glula and glulb in zebrafish hair cell formation and auditory function.
SNHG15 Mediates MTSS1 Gene Expression via Interacting with the Gene Promoter and Regulating Transcription Pausing
Authors: Zhou Yanchun , Chen Shaoying , Chen Weibin , Wu Jundong , Gu Wei
Published: October 2024

Metastasis suppressor 1 (MTSS1) has been reported to play important roles in suppressing cancer progression. In this study, we investigated the underlying mechanism that regulates MTSS1 expression. We showed that in breast cancer cells, lncRNA-SNHG15-induced cell invasion and proliferation was accompanied with the decreased expression of MTSS1 mRNA. Further study revealed that SNHG15 mediated MTSS1 repression through blocking its promoter activity. Mechanistically, SNHG15 complexes with DDX5 and RTF1 and interacts with the core promoter of the MTSS1 gene to interfere with RNA-Pol-II-directed transcriptional initiation. Association with DDX5 stabilizes SNHG15 while binding to RTF1 allows SNHG15 to carry RTF1 to the core promoter, where RTF1 forms a complex with PNA pl II to enhance transcriptional pausing. Our findings revealed a molecular mechanism by which SNHG15 serves as a regulator to suppresses MTSS1 transcription via interaction with the gene core promoter.
Unraveling the Molecular Mechanisms of SIRT7 in Angiogenesis: Insights from Substrate Clues
Authors: Ma Junjie , Yang Liqian , Wu Jiaxing , Huang Zhihong , Zhang Jiaqi , Liu Minghui , Li Meiting , Luo Jianyuan , Wang Haiying
Published: October 2024

Angiogenesis, a vital physiological or pathological process regulated by complex molecular networks, is widely implicated in organismal development and the pathogenesis of various diseases. SIRT7, a member of the Sirtuin family of nicotinamide adenine dinucleotide + (NAD+) dependent deacetylases, plays crucial roles in cellular processes such as transcriptional regulation, cell metabolism, cell proliferation, and genome stability maintenance. Characterized by its enzymatic activities, SIRT7 targets an array of substrates, several of which exert regulatory effects on angiogenesis. Experimental evidence from in vitro and in vivo studies consistently demonstrates the effects of SIRT7 in modulating angiogenesis, mediated through various molecular mechanisms. Consequently, understanding the regulatory role of SIRT7 in angiogenesis holds significant promise, offering novel avenues for therapeutic interventions targeting either SIRT7 or angiogenesis. This review delineates the putative molecular mechanisms by which SIRT7 regulates angiogenesis, taking its substrates as a clue, endeavoring to elucidate experimental observations by integrating knowledge of SIRT7 substrates and established angiogenenic mechanisms.
Challenges Associated with Smooth Muscle Tumor of Uncertain Malignant Potential (STUMP) Management—A Case Report with Comprehensive Literature Review
Authors: Kwiatkowski Jakub , Akpang Nicole , Zaborowska Lucja , Grzelak Marcelina , Lukasiewicz Iga , Ludwin Artur
Published: October 2024

Background: Smooth Muscle Tumor of Uncertain Malignant Potential (STUMP) is a poorly studied neoplasm that does not fulfill the definition of either leiomyoma or leiomyosarcoma. STUMP symptoms are indistinguishable from those of benign lesions; it has no specific biochemical markers or ultrasound presentations. The management of this type of tumor is particularly challenging due to significant heterogeneity in its behavior and the lack of clear guidelines; moreover, the lesion may recur after excision. Case Report: We report on a case of a 42-year-old patient diagnosed with a STUMP. The preliminary diagnosis was a submucous leiomyoma, which was removed hysteroscopically due to menorrhagia resulting in anemia. The histopathological examination of the resected myoma pointed to the diagnosis of STUMP. The hysterectomy was performed as the patient had completed her reproductive plans. There were no complications. The patient is currently recurrence-free after a 9-month follow-up. Discussion and Conclusions: The care of a patient diagnosed with STUMP requires a personalized approach and the cooperation of various medical disciplines, including molecular diagnostics, imaging techniques, and minimally invasive surgery. Management of STUMP must consider the patient’s plans for childbearing. All cases of tumors with “uncertain malignant potential” are a challenge in the context of patient-physician communication.
A Review of Simulation Tools for Thin-Film Solar Cells
Authors: Salgado-Conrado Lizbeth , Álvarez-Macías Carlos , Reyes-Durán Bernardo
Published: October 2024

Unlike current silicon-based photovoltaic technology, the development of last-generation thin-film solar cells has been marked by groundbreaking advancements in new materials and novel structures to increase performance and lower costs. However, physically building each new proposal to evaluate the device’s efficiency can involve unnecessary effort and time. Numerical simulation tools provide a solution by allowing researchers to predict and optimize solar cell performance without physical testing. This paper reviews thirteen of the main numerical simulation tools for thin-film solar cells, including SCAPS, AMPS, AFORS-HET, ASPIN3, GPVDM, SESAME, SILVACO, SENTAURUS, and ADEPT. This review evaluates each tool’s features, modeling methods, numerical approaches, and application contexts. The findings reveal notable differences in material modeling, numerical accuracy, cost, and accessibility among the tools. Each tool’s strengths and limitations in simulating thin-film solar cells are highlighted. This study emphasizes the necessity of selecting suitable simulation tools based on specific research requirements. It provides a comparative analysis to assist researchers in choosing the most effective software for optimizing thin-film solar cells, contributing to advancements in photovoltaic technology.
Current-Induced Field-Free Switching of Co/Pt Multilayer via Modulation of Interlayer Exchange Coupling and Magnetic Anisotropy
Authors: Kim Byungro , Seo Dongpyo , Yoon Seungha , Han Songhee , Kim Taeheon , Cho Beongki
Published: October 2024

Current-induced field-free magnetic switching using spin–orbit torque has been an important topic for decades due to both academic and industrial interest. Most research has focused on introducing symmetry breakers, such as geometrical and compositional variation, pinned layers, and symmetry-broken crystal structures, which add complexity to the magnetic structure and fabrication process. We designed a relatively simple magnetic structure, composed of a [Co/Pt] multilayer and a Co layer with perpendicular and in-plane magnetic anisotropy, respectively, with a Cu layer between them. Current-induced deterministic magnetic switching was observed in this magnetic system. The system is advantageous due to its easy control of the parameters to achieve the optimal condition for magnetic switching. The balance between magnetic anisotropic strength and interlayer coupling strength is found to provide the optimal condition. This simple design and easy adjustability open various possibilities for magnetic structures in spin-based electronics applications using spin–orbit torque.
A New Feasible Opportunity for Recycling Lead and Silver from Zinc Plant Residues by Flotation
Authors: Kamran Haghighi Hossein , Hoseinian Fatemeh Sadat , Maria Sastre Ana
Published: October 2024

Millions of tons of zinc plant leach residues (ZPLR) have been stockpiled in Iranian hydrometallurgical zinc plants during the last few decades. Due to the low grades of zinc, lead, and silver in these residues, these residues have been abandoned without treatment. The authors of this paper studied zinc plant leach residues (ZPLR) to propose a flotation process for separating and producing lead and silver concentrate. A response surface methodology (RSM) was employed to obtain six models for optimizing the best conditions for lead recovery, lead grade, zinc recovery, zinc grade, silver recovery, and silver grade. In these models, the effect of the different main variables, including density, flotation time, pH, sodium sulfide dosage, and potassium amyl xanthate dosage, was investigated to optimize grades and recoveries. The studied ZPLRs were categorized into two types based on the disposal time, including new and old residues. The chemical analysis showed that the grades of lead, zinc, and silver in the new residues are higher than in the old residues. In a previous mineralogical study, it was found that silver forms in lead and zinc minerals as a solid solution within their structures. The resulting 3D graphs showed that the interacting variables have significant effects on responses. The ANOVA analysis exhibited the order of model significance to be lead grade (F-value of 36.46) > silver grade (19.76) > lead recovery (7.88) > zinc grade (5.63) > silver recovery (5.58) > zinc recovery (4.83). Based on these models, under the conditions of 1126.26 g/cm3 density, 20.83 min retention time, 9.9 pH, 6 kg/t sodium sulfide, and 749.66 g/t potassium amyl xanthate dosage for a new residue type, the recoveries of lead, zinc, and silver were determined to be 51.10%, 11.13%, and 72.85%, with grades of 38.87% Pb, 8.46% Zn, and 1209.11 g/t Ag, respectively. According to the feasibility study results, the presented work is reasonable in terms of technical, economic, and investment potential.
Directionally Oriented Reinforcements of Warp-Knitted Fabrics for Composite Preforms
Authors: Pieklak Katarzyna
Published: October 2024

This paper focuses on the development of a methodology for the directional structural modification of warp-knitted fabrics by sewing on carbon fiber tapes. Four-, five-, and six-axial geometric systems were designed to optimize the qualitative distribution of stresses on the surface of the tested product. Through a numerical experiment in the ANSYS environment, the impact of the change in the axiality of a textile structure on the mechanical properties of the modeled geometric configuration was assessed. This analysis was experimentally verified by measuring the multiaxial force distribution on the knitted surface, which demonstrated that Variant 7, with six axes 30° apart, was the most favorable.
Investigation of Tensile Properties and In Situ Analysis of Fracture Behavior in High-Porosity Open-Cell Nickel Foam
Authors: Fan Sufeng , Wang Xihai , Kong Zhe , Hou Qinghua
Published: October 2024

Nickel foam offers excellent conductivity, a high surface area, and lightweight structure, making it ideal for applications, like battery electrodes, catalysts, and filtration systems. Its durability and corrosion resistance further enhance its performance in various industries. However, few studies focus on the tensile anisotropy of nickel foam and its tensile fracture process. In this study, the anisotropic tensile behavior of nickel foam with varying relative densities has been investigated, along with its tensile fracture behavior using in situ techniques. The tensile properties of nickel foams show strong anisotropy due to the flattening process in the production process. The results show that the tensile properties, including the yield strength, tensile strength, and elastic modulus, increase with the increasing relative density, while the elongation percentage has no relationship with the relative density. The experiment data on tensile strength are in agreement with Gibson’s formula and Liu’s formula. In situ tensile tests are conducted to explore the microscopic fracture mechanism of nickel foam. The results show that the struts of nickel foam are tensile fractures or shear fractures near the joints, and the fracture process of struts is clearly recorded and analyzed. This study is significant as it provides critical insights into the anisotropic tensile behavior of nickel foam and fracture mechanism, enabling the optimization of production processes and broadening its potential applications.
Integrated Control of Thermal Residual Stress and Mechanical Properties by Adjusting Pulse-Wave Direct Energy Deposition
Authors: Yan Zhou , Guo Jia , Zou Xi , Wang Siyu
Published: October 2024

Directed energy deposition with laser beam (DED-LB) components experience significant residual stress due to rapid heating and cooling cycles. Excessive residual tensile stress can lead to cracking in the deposited sample, resulting in service failure. This study utilized digital image correlation (DIC) and thermal imaging to observe the in situ temporal evolution of strain and temperature gradients across all layers of a deposited 316 L stainless steel thin wall during DED-LB. Both continuous-wave (CW) and pulsed-wave (PW) laser modes were employed. Additionally, the characteristics of thermal cracks and geometric dislocation density were examined. The results reveal that PW mode generates a lower temperature gradient, which in turn reduces thermal strain. In CW mode, the temperature–stress relationship curve of the additive manufacturing sample enters the “brittleness temperature zone”, leading to the formation of numerous hot cracks. In contrast, PW mode samples are almost free of cracks, as the metal avoids crack-sensitive regions during solidification, thereby minimizing hot crack formation. Overall, these factors collectively contribute to reduced residual stress and improved mechanical properties through the adjustment of pulsed-wave laser deposition.
Effect of rPET Content and Preform Heating/Cooling Conditions in the Stretch Blow Molding Process on Microcavitation and Solid-State Post-Condensation of vPET-rPET Blend: Part I—Research Methodology and Results
Authors: Wawrzyniak Paweł , Karaszewski Waldemar , Różański Artur
Published: October 2024

Polyethylene terephthalate (PET) is widely used in bottle production due to its cost-effectiveness and low environmental impact. The first part of this article describes the research and statistical analysis methodology of the influence of the virgin PET (vPET) and recycled PET (rPET) content in the vPET-rPET blend, as well as the preform heating/cooling conditions in the stretch blow molding (SBM) process on the microscopic bottle properties. Microscopic properties such as crystallinity, density, viscosity, relaxation degree of the amorphous phase, and microcavitation in PET were examined. This study reveals that microcavity and solid-state post-condensation effects occur during PET deformation in the SBM process. The increase in free volume, indicating microcavitation, was confirmed by measuring positron annihilation lifetime spectroscopy (PALS). PALS and density of the amorphous phase studies prove a reduction in the dimensions of the free volumes, with a simultaneous significant increase in their number and ellipsoidization. It can be associated with crystallite rotation in a temperature-dependent non-crystalline matrix. The occurrence of solid-state post-condensation effects was confirmed by measuring the intrinsic viscosity. The conclusions resulting from the analysis of the microstructure affecting the mechanical strength of the material were validated by pressure resistance tests of the bottles.
Implementing the Design of Experiments (DoE) Concept into the Development of Mucoadhesive Tablets Containing Orange Peel Extract as a Potential Concept for the Treatment of Oral Infections
Authors: Paczkowska-Walendowska Magdalena , Karpiński Tomasz M. , Garbiec Ewa , Walendowski Michał , Cielecka-Piontek Judyta
Published: October 2024

This study explores for the first time the impact of chitosan (CS) with varying molecular weights (MW), orange peel extract concentration, and hydroxypropyl methylcellulose (HPMC) content on the formulation of buccal tablets for treating oral infections. Utilizing a statistical design of experiments (DoE), nine different formulations were evaluated for mechanical properties, dissolution behavior, mucoadhesion, and biological activity. A formulation with high CS MW, 60% orange peel extract, and 8% HPMC, emerged as the optimal formulation, demonstrating superior tabletability, compressibility, and compactibility. Dissolution studies indicated that hesperidin release followed the Higuchi model, with higher extract content enhancing this phenomenon. Mucoadhesion improved with increased HPMC and CS concentrations, although higher extract content reduced bioadhesion. Biological assays showed that higher extract levels boosted antioxidant activity, while CS primarily contributed to anti-inflammatory effects. The optimized formulation exhibited broad antimicrobial activity against key oral pathogens, surpassing the effectiveness of the individual components. Principal component analysis (PCA) further confirmed the significant influence of extract content on tablet properties. These findings suggest that the optimized tablet formulation holds promise for effective buccal delivery in the treatment of oral infections, warranting further investigation in clinical settings.
Direct Preparation of Alginate Oligosaccharides from Brown Algae by an Algae-Decomposing Alginate Lyase AlyP18 from the Marine Bacterium Pseudoalteromonas agarivorans A3
Authors: Sun Xiao-Hui , Zhang Xiao-Dong , Zhang Xin-Ru , Wang Xiao-Fei , Zhang Xi-Ying , Zhang Yu-Zhong , Zhang Yu-Qiang , Xu Fei
Published: October 2024

Alginate oligosaccharides (AOs), derived from alginate degradation, exhibit diverse biological activities and hold significant promise in various fields. The enzymatic preparation of AOs relies on alginate lyases, which offers distinct advantages. In contrast to the conventional use of sodium alginate derived from brown algae as the substrate for the enzymatic preparation of AOs, AO preparation directly from brown algae is more appealing due to its time and energy efficiency. Thus, the identification of potent alginate lyases and cost-effective brown algae substrates is crucial for optimizing AO production. Herein, we identified and characterized an alginate lyase, AlyP18, capable of efficiently decomposing algae, from a marine bacterium Pseudoalteromonas agarivorans A3 based on secretome analysis. AlyP18 is a mesothermal, endo-type and bifunctional alginate lyase with high enzymatic activity. Two brown algae substrates, Laminaria japonica roots and Macrocystis pyrifera, were used for the AO preparation by AlyP18. Upon optimization of AlyP18 hydrolysis parameters, the substrate degradation efficiency and AO production reached 53% and ~32% for L. japonica roots, respectively, and 77% and ~46.5% for M. pyrifera. The generated AOs primarily consisted of dimers to pentamers, with trimers and tetramers being dominant. This study provides an efficient alginate lyase and alternative brown algal feedstock for the bioconversion of high-value AOs from brown algae.
The Effectiveness of Heat-Killed Pediococcus acidilactici K15 in Preventing Respiratory Tract Infections in Preterm Infants: A Pilot Double-Blind, Randomized, Placebo-Controlled Study
Authors: Takeshita Kenichi , Hishiki Haruka , Takei Haruka , Ikari Naho , Tanaka Saori , Iijima Yuta , Ogata Hitoshi , Fujishiro Kensuke , Tominaga Takahiro , Konno Yuki , Iwase Yukiko , Nakano Taiji , Endo Mamiko , Ishiwada Naruhiko , Osone Yoshiteru , Kawaguchi Tomohiro , Horiba Taro , Takemura Ryo , Hamada Hiromichi , Shimojo Naoki
Published: October 2024

Background: Preterm infants discharged from the neonatal intensive care unit (NICU) have a risk of severe viral respiratory tract infections (RTIs). Researchers have recently reported the potential use of postbiotics to decrease RTIs in young children. However, the safety and efficacy of postbiotics for preventing RTIs in preterm infants is not yet established. Methods: We conducted a pilot double-blind, randomized, placebo-controlled study of the heat-killed lactic acid bacterium Pediococcus acidilactici K15 in 41 preterm infants born at <36 weeks of gestation and discharged from the NICU at Chiba University Hospital. Results: Following once-daily K15 or placebo treatment for one year, no significant differences were found in the mean number of febrile days (4.5 [1.5–7.4] days vs. 6.6 [2.6–10.5] days). The subgroup analysis showed that the effect of treatment on the number of febrile days was more prominent in the K15 group than in the placebo group, among children with older siblings. The 16S rRNA gene sequencing of fecal samples illustrated that the genus Faecalimonas was enriched in the K15 group, potentially promoting butyrate production by butyrate-producing bacteria. No adverse events were found to be associated with K15 intake. Conclusion: There were no clear data to show the effectiveness of K15 in preventing fever and RTIs in preterm babies during infancy. A larger clinical trial is warranted.
Beneficial Effects of a Freeze-Dried Kale Bar on Type 2 Diabetes Patients: A Randomized, Double-Blinded, Placebo-Controlled Clinical Trial
Authors: Jeppesen Per , Dorner Amanda , Yue Yuan , Poulsen Nikolaj , Andersen Sofie , Aalykke Fie , Lambert Max
Published: October 2024

Background/Objectives: Type 2 diabetes (T2D) is one of the most common global diseases, with an ever-growing need for prevention and treatment solutions. Kale (Brassica oleracea L. var. acephala) offers a good source of fiber, minerals, bioavailable calcium, unsaturated fatty acids, prebiotic carbohydrates, vitamins, health-promoting secondary plant metabolites, as well as higher amounts of proteins and essential amino acids compared to other vegetables. The objective of this study was to investigate whether daily intake of freeze-dried kale powder can provide health benefits for T2D patients vs. placebo. Methods: This study was designed as a 12-week, blinded, randomized, controlled trial. Thirty T2D patients were randomly assigned to either a placebo bar (control) or a kale bar (intervention). Participants in the intervention group were instructed to consume three bars/day, each containing 26.25 g of freeze-dried kale (corresponding to approx. 341 g fresh kale/day). At baseline and 12 weeks, all participants underwent an oral glucose tolerance test (OGTT), 24 h blood pressure measurements, DEXA scans, and fasted blood samples were taken. Results: A significant reduction in HbA1c, insulin resistance, body weight, and calorie intake was observed in the intervention group compared to control. Positive trends were detected in fasted blood glucose and LDL-cholesterol for those in the kale intervention group. No significant differences were found in total body fat mass and area under the curve glucose 240 min OGTT. Conclusions: Given the positive effects of high daily kale intake observed in this study, further research with a larger sample size is needed to better understand the health benefits of kale bars. This could potentially lead to new dietary recommendations for patients with T2D.
The Association Between Dietary Vitamin C and Sleep Disorders: A Cohort Study Based on UK Biobank
Authors: Zhang Qiuge , Qi Xueting , Wang Zhaoguo , Zhang Dongfeng , Wang Tong
Published: October 2024

Objective: Approximately 30% of adults globally suffer from sleep disorders. However, there are few longitudinal studies on the association between dietary vitamin C and sleep disorders. This study aimed to investigate the association between dietary vitamin C intake and various types of sleep disorders, including sleep apnea and insomnia. Methods: We enrolled 68,221 participants from the UK Biobank. Dietary vitamin C intake was assessed using a 24 h dietary recall questionnaire. We employed a Cox regression model to assess the association between dietary vitamin C and sleep disorders and used restricted cubic spline models to investigate potential nonlinear relationships. Stratified and sensitivity analyses were also conducted to validate the findings. Results: The results indicated that vitamin C intake at the Q4 level (132.88–191.51 mg/d) was significantly associated with a reduced risk of sleep disorders, with an HR (95% CI) of 0.78 (0.65–0.94), and against sleep apnea, with an HR (95% CI) of 0.75 (0.62–0.92). The protective effect persisted significant in stratified analyses of men and those aged ≤ 60 years; the results were consistent in the sensitivity analyses. Conclusions: Our findings suggest that sufficient dietary vitamin C intake may help to prevent sleep disorders.
Preparation Process Optimization of Glycolipids from Dendrobium officinale and the Difference in Antioxidant Effects Compared with Ascorbic Acid
Authors: Long Yan , Yang Jiajing , Ji Hongfei , Han Xiao , Fan Yuting , Dai Keyao , Ji Haiyu , Yu Juan
Published: October 2024

Background:Dendrobium officinale glycolipids (DOG), often left as residues after hot water extraction for polysaccharide production, are often discarded. Methods: This study investigates the optimal extraction of DOG using response surface methodology, focusing on liquid–solid ratios, ethanol concentrations, extraction temperatures, and extraction times, while preliminarily analyzing DOG’s structural properties. Additionally, the differences in antioxidant effects between DOG and ascorbic acid based on intestinal flora metabolism were further evaluated. Results: The optimal parameters for DOG extraction were determined as follows: liquid–solid ratio of 20 mL/g, ethanol concentration of 70%, extraction temperature of 70 °C, and extraction time of 2.5 h, yielding 2.64 ± 0.18%. In addition, DOG was identified as a diglyceride, mainly composed of glucose, mannose, linoleic acid, 9,12,15-octadecatrienoic acid, and presented certain direct free radicals scavenging effects. In animal experiments, unlike the direct free scavenging effects of ascorbic acid, DOG increased intestinal Bacteroides acidifaciens abundance in mice, up-regulated piceatannol expression, and down-regulated 1-naphthol expression, which contributed to antioxidant effects by enhancing the activities of SOD and GSH-Px while reducing MDA content. Conclusions: DOG was a diglyceride isolated from D. officinale residues after hot water extraction, and presented strong antioxidant effects by regulating intestinal flora metabolism. These findings could promote the efficient utilization of D. officinale and support further development of DOG in functional food applications.
Relationship Between Vitamin D Insufficiency and Anemia in Older Adults: An Approach Considering Clinical Aspects and Food Insecurity
Authors: Mesquita Maria Cecília Cougo , de Castro Rafaela Martins , Mendes Talissa Vicente , do Carmo Mariana Araújo Vieira , Sampaio Eliza de Souza , Corona Ligiana Pires , Lima Daniela Braga , Raposo António , Alasqah Ibrahim , Alqarawi Nada , Albaridi Najla A. , Alsharari Zayed D. , de Brito Tábatta Renata Pereira
Published: October 2024

Background/Objectives: Studies have shown a high prevalence of anemia and vitamin D insufficiency in older adults, and the literature suggests a relationship between these two conditions, as vitamin D insufficiency may impair erythrocyte synthesis. Food insecurity refers to the lack of regular access to sufficient and nutritious food, which can directly affect health by worsening conditions such as anemia and vitamin D insufficiency. This study evaluated the association between vitamin D insufficiency and anemia in older adults. Methods: We conducted a cross-sectional study with 430 individuals aged 60 and older, using personal interviews and blood tests for data collection. Anemia was identified with serum hemoglobin levels of <12 g/dL for women and <13 g/dL for men, while vitamin D insufficiency was defined as serum levels <30 ng/mL. We used multiple logistic regression to analyze associations through Stata version 17.0 software. Results: The prevalence of anemia was identified in 14.7% of the sample, and vitamin D insufficiency was observed in 63.5%. We found an association between vitamin D insufficiency and anemia (OR = 2.4; 95% CI = 1.2–4.7). In the final model, factors such as male sex (OR = 2.7; 95% CI = 1.5–4.9) and polypharmacy use (OR = 2.0; 95% CI = 1.0–3.9) were also associated, regardless of age group, food insecurity, and multimorbidity. Conclusions: Vitamin D insufficiency increased the likelihood of anemia among the older adults evaluated, suggesting that prevention and treatment strategies for anemia should consider vitamin D serum levels.
Exploring the ‘Pet Effect’: Does Playing with Pets Contribute to Owner Wellbeing?
Authors: Gradidge Sarah , Loughnan Steve , Gibson Nic
Published: October 2024

Although many people strongly believe in wellbeing benefits of having pets (the ‘pet effect’), research on the ‘pet effect’ in actuality is highly mixed. Surprisingly, little research has explored the nature of (i.e., specific components of) the human–pet relationship, as a way to explain the contradictory findings. One such component is human–pet play, with play outside of the human–pet relationship being important for wellbeing and social buffering. Thus, the current quantitative study explores whether greater perceived play with pets contributes to greater wellbeing (‘pet effect’) and reduces anxiety during a time of acute stress (social buffering). The study employs a regression design recruiting men and women residing in the UK during the COVID-19 pandemic who own a dog and/or cat, with perceived pet play as the key predictor variable, and outcome variables of wellbeing and COVID-19 anxiety. Data were gathered and analysed cross-sectionally on day one (N = 189), and longitudinally over five days (N = 105), using multiple regressions. Overall, perceived pet play did not predict wellbeing nor COVID-19 anxiety. As such, the current study indicates human–pet play does not contribute to the ‘pet effect’ nor social buffering, thus raising questions for future research regarding the exact purpose of play within the human–pet relationship.
Molecular Docking, Bioinformatic Analysis, and Experimental Verification for the Effect of Naringin on ADHD: Possible Inhibition of GSK-3β and HSP90
Authors: Mokhtar Hatem I. , Zaitone Sawsan A. , El-Sayed Karima , Lashine Rehab M. , Ahmed Nada , Moursi Suzan M. M. , Shehata Shaimaa A. , Aldahish Afaf A. , Helal Mohamed A. , El-Kherbetawy Mohamed K. , Fawzy Manal S. , Abd El-Fadeal Noha M.
Published: October 2024

Background/Objectives: One of the most abundant and growing neurodevelopmental disorders in recent decades is attention deficit hyperactivity disorder (ADHD). Many trials have been performed on using drugs for the improvement of ADHD signs. This study aimed to detect the possible interaction of naringin with Wnt/β-catenin signaling and its putative anti-inflammatory and protective effects in the mouse ADHD model based on bioinformatic, behavioral, and molecular investigations. Furthermore, molecular docking was applied to investigate possible interactions with the GSK-3β and HSP90 proteins. Methods: Male Swiss albino mice were divided into four groups, a normal control group, monosodium glutamate (SGL) control, SGL + naringin 50 mg/kg, and SGL + naringin 100 mg/kg. The psychomotor activity of the mice was assessed using the self-grooming test, rope crawling test, and attentional set-shifting task (ASST). In addition, biochemical analyses were performed using brain samples. Results: The results of the SGL group showed prolonged grooming time (2.47-folds), a lower percentage of mice with successful crawling on the rope (only 16.6%), and a higher number of trials for compound discrimination testing in the ASST (12.83 ± 2.04 trials versus 5.5 ± 1.88 trials in the normal group). Treatment with naringin (50 or 100 mg per kg) produced significant shortening in the grooming time (31% and 27% reductions), as well as a higher percentage of mice succeeding in crawling with the rope (50% and 83%, respectively). Moreover, the ELISA assays indicated decreased dopamine levels (0.36-fold) and increased TNF-α (2.85-fold) in the SGL control group compared to the normal mice, but an improvement in dopamine level was observed in the naringin (50 or 100 mg per kg)-treated groups (1.58-fold and 1.97-fold). Similarly, the PCR test showed significant declines in the expression of the Wnt (0.36), and β-catenin (0.33) genes, but increased caspase-3 (3.54-fold) and BAX (5.36-fold) genes in the SGL group; all these parameters were improved in the naringin 50 or 100 mg/kg groups. Furthermore, molecular docking indicated possible inhibition for HSP90 and GSK-3β. Conclusions: Overall, we can conclude that naringin is a promising agent for alleviating ADHD symptoms, and further investigations are required to elucidate its mechanism of action.
Ionospheric Absorption Variation Based on Ionosonde and Riometer Data and the NOAA D-RAP Model over Europe During Intense Solar Flares in September 2017
Authors: Barta Veronika , Bozóki Tamás , Süle Dávid Péter , Kouba Daniel , Mielich Jens , Raita Tero , Buzás Attila
Published: October 2024

A novel method was developed based on the amplitude data of the EM waves measured by Digisondes to calculate and investigate the relative ionospheric absorption changes. The effect of 13 solar flares (>C8) that occurred from 4 to 10 September 2017 were studied at three European Digisonde stations (Juliusruh (54.63°N, 13.37°E), Průhonice (49.98°N, 14.55°E) and San Vito (40.6°N, 17.8°E)). The present study compares the results of the amplitude method with the absorption changes measured by the Finnish Riometer Network and determined by the NOAA D-RAP model during the same events. The X-class flares caused 1.5–2.5 dB of attenuation at 30–32.5 MHz based on the riometer data, while the absorption changes were between 10 and 15 dB in the 2.5–4.5 MHz frequency range according to the amplitude data. The impact caused by energetic particles after the solar flares are clearly seen in the riometer data, while among the Digisonde stations it can be observed only at Juliusruh in some certain cases. Comparing the results of the amplitude method with the D-RAP model it seems evident that the observed absorption values almost always exceed the values given by the model both at 2.5 MHz and at 4 MHz during the investigated period. According to the comparison between the riometer data with the D-RAP, generally, the model underestimates the absorption values obtained from the riometers during solar flares except at the highest latitude stations, while D-RAP overestimates the impact during the particle events.
The Lunar Regolith Thickness and Stratigraphy of the Chang’E-6 Landing Site
Authors: Li Jin , Yin Chengxiang , Chi Siyue , Mao Wenshuo , Fu Xiaohui , Zhang Jiang
Published: October 2024

The Chang’E-6 (CE-6) mission successfully returned 1935.3 g of lunar soil samples from the Apollo basin within the South Pole–Aitken basin. One of its scientific objectives is to investigate the subsurface structure and regolith thickness at the landing site. Using remote sensing datasets, we estimated the regolith and basalt thicknesses at the landing site by employing the crater morphology method and crater excavation technique. A total of 53 concentric craters and 108 fresh craters with varying excavation depths were identified. Our results indicate that the regolith thickness at the CE-6 landing site ranges from 1.1 to 7.0 m, with an average thickness of 3.5 m. Beneath the regolith, the basalt layer consists of high-Ti basalt overlaying low-Ti basalt, with a total thickness of approximately 64 to 82 m, of which the high-Ti basalt layer accounts for about 22 to 30 m. Based on the local geological history, we proposed a stratigraphy at the CE-6 landing site. These findings provide valuable geological context for interpreting the Lunar Penetrating Radar data and analyzing the returned samples.
Exploring Spatio-Temporal Dynamics of Future Extreme Precipitation, Runoff, and Flood Risk in the Hanjiang River Basin, China
Authors: Wang Dong , Shao Weiwei , Liu Jiahong , Su Hui , Zhang Ga , Fu Xiaoran
Published: October 2024

The hydrological cycle is altered by climate change and human activities, amplifying extreme precipitation and heightening the flood risk regionally and globally. It is imperative to explore the future possible alterations in flood risk at the regional scale. Focusing on the Hanjiang river basin (HRB), this study develops a framework for establishing a scientific assessment of spatio-temporal dynamics of future flood risks under multiple future scenarios. In this framework, a GCMs statistical downscaling method based on machine learning is used to project future precipitation, the PLUS model is used to project future land use, the digitwining watershed model (DWM) is used to project future runoff, and the entropy weight method is used to calculate risk. Six extreme precipitation indices are calculated to project the spatio-temporal patterns of future precipitation extremes in the HRB. The results of this study show that the intensity (Rx1day, Rx5day, PRCPTOT, SDII), frequency (R20m), and duration (CWD) of future precipitation extremes will be consistently increasing over the HRB during the 21st century. The high values of extreme precipitation indices in the HRB are primarily located in the southeast and southwest. The future annual average runoff in the upper HRB during the near-term (2023–2042) and mid-term (2043–2062) is projected to decrease in comparison to the baseline period (1995–2014), with the exception of that during the mid-term under the SSP5-8.5 scenario. The high flood risk center in the future will be distributed in the southwestern region of the upper HRB. The proportions of areas with high and medium–high flood risk in the upper HRB will increase significantly. Under the SSP5-8.5 scenario, the area percentage with high flood risk during the future mid-term will reach 24.02%. The findings of this study will facilitate local governments in formulating effective strategic plans for future flood control management.
CLOUDSPAM: Contrastive Learning On Unlabeled Data for Segmentation and Pre-Training Using Aggregated Point Clouds and MoCo
Authors: Mahmoudi Kouhi Reza , Stocker Olivier , Giguère Philippe , Daniel Sylvie
Published: October 2024

SegContrast first paved the way for contrastive learning on outdoor point clouds. Its original formulation targeted individual scans in applications like autonomous driving and object detection. However, mobile mapping purposes such as digital twin cities and urban planning require large-scale dense datasets to capture the full complexity and diversity present in outdoor environments. In this paper, the SegContrast method is revisited and adapted to overcome its limitations associated with mobile mapping datasets, namely the scarcity of contrastive pairs and memory constraints. To overcome the scarcity of contrastive pairs, we propose the merging of heterogeneous datasets. However, this merging is not a straightforward procedure due to the variety of size and number of points in the point clouds of these datasets. Therefore, a data augmentation approach is designed to create a vast number of segments while optimizing the size of the point cloud samples to the allocated memory. This methodology, called CLOUDSPAM, guarantees the performance of the self-supervised model for both small- and large-scale mobile mapping point clouds. Overall, the results demonstrate the benefits of utilizing datasets with a wide range of densities and class diversity. CLOUDSPAM matched the state of the art on the KITTI-360 dataset, with a 63.6% mIoU, and came in second place on the Toronto-3D dataset. Finally, CLOUDSPAM achieved competitive results against its fully supervised counterpart with only 10% of labeled data.