bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
Authors: Xu Kaida , Xu Hanxiang , Han Zhiqiang
Published: November 2018

Heat shock proteins 70 (Hsp70) are required for key cellular processes and responses to environmental changes, however, there are an unknown number of hsp70 gene family members in the large yellow croaker (Larimichthys crocea). In the present study, 17 hsp70 genes were identified through the genome of the large yellow croaker. These genes are divided into seven evolutionarily distinct groups according to a phylogenetic tree. The orthologs of these hsp70 genes were found in humans and zebrafish. The expression patterns of the hsp70 gene family in the large yellow croaker under cold and heat stress were studied by examining transcriptome data. Six out of 17 genes were significantly unregulated or downregulated after cold or heat stress. There were two genes significantly upregulated and two genes downregulated in the liver after cold treatment, while after heat treatment, five genes were significantly upregulated, and no genes were significantly downregulated. Three expression patterns were detected: strictly heat-inducible hsp70, constitutively expressed and moderately heat-inducible hsp70, and constitutively expressed and less stress-dependent hsp70 genes. All the findings will contribute to a better understanding of the biological function of hsp70s in defending against thermal challenges.
Hope for the Best, Prepare for the Worst—An Assessment of Flood Preparedness at Primary Health Care Facilities in Central Vietnam
Authors: Älgå Andreas , Dang Thi Anh Thu , Saulnier Dell D. , Nguyen Gia Thanh , Von Schreeb Johan
Published: November 2018

Background: Floods affect over 85 million people every year and are one of the deadliest types of natural disasters. The health effects of floods are partly due to a loss of access to health care. This loss can be limited with proper flood preparedness. Flood preparedness is especially needed at the primary health care (PHC) level. Flood preparedness assessments can be used to identify vulnerable facilities and help target efforts. The existing research on PHC flood preparedness is limited. We aimed to assess the flood preparedness of PHC facilities in a flood-prone province in central Vietnam. Methods: Based on flood experience, the PHC facilities in the province were grouped as “severe” (n = 23) or “non-severe” (n = 129). Assessments were conducted during monsoon season at five facilities from each group, using a pre-tested, semi-structured questionnaire. Data were checked against official records when possible. Results: Nine of the ten facilities had a flood plan and four received regular flood preparedness training. Six facilities reported insufficient preparedness support. Half of the facilities had additional funding available for flood preparedness, or in case of a flood. Flood preparedness training had been received by 21/28 (75%) of the staff at the facilities with severe flood experience, versus 15/25 (52%) of the staff at the non-severe experience facilities. Conclusions: Our results suggest that the assessed PHC facilities were not sufficiently prepared for the expected floods during monsoon season. PHC flood preparedness assessments could be used to identify vulnerable facilities and populations in flood-prone areas. More research is needed to further develop and test the validity and reliability of the questionnaire.
Biomarkers of Exposure to Secondhand and Thirdhand Tobacco Smoke: Recent Advances and Future Perspectives
Authors: Torres Sònia , Merino Carla , Paton Beatrix , Correig Xavier , Ramírez Noelia
Published: November 2018

Smoking is the leading preventable disease worldwide and passive smoking is estimated to be the cause of about 1.0% of worldwide mortality. The determination of tobacco smoke biomarkers in human biological matrices is key to assess the health effects related to the exposure to environmental tobacco smoke. The biomonitoring of cotinine, the main nicotine metabolite, in human biofluids—including urine, serum or saliva—has been extensively used to assess this exposure. However, the simultaneous determination of cotinine together with other tobacco biomarkers and the selection of alternative biological matrices, such as hair, skin or exhaled breath, would enable a better characterization of the kind and extent of tobacco exposure. This review aims to perform a critical analysis of the up-to-date literature focused on the simultaneous determination of multiple tobacco smoke biomarkers studied in different biological matrices, due to the exposure to secondhand smoke (SHS) and thirdhand smoke (THS). Target biomarkers included both tobacco-specific biomarkers—nicotine and tobacco specific nitrosamine biomarkers—and tobacco-related biomarkers, such as those from polycyclic aromatic hydrocarbons, volatile organic compounds, metals and carbon monoxide. To conclude, we discuss the suitability of determining multiple biomarkers through several relevant examples of SHS and THS exposure.
Personal Network Analysis in the Study of Social Support: The Case of Chronic Pain
Authors: Fernández-Peña Rosario , Molina José Luis , Valero Oliver
Published: November 2018

In the context of chronic illness, the individual’s social and relational environment plays a critical role as it can provide the informal support and care over time, beyond healthcare and social welfare institutions. Social Network Analysis represents an appropriate theoretical and methodological approach to study and understand social support since it provides measures of personal network structure, composition and functional content. The aim of this mixed method study is to present the usefulness of Personal Network Analysis to explore social support in the context of chronic pain. Personal and support network data of 30 people with chronic pain (20 alters for each ego, 600 relationships in total) were collected, obtaining measures of personal network structure and composition as well as information about social support characteristics. Also, semi-structured interviews with participants were conducted to identify the context of their experience of pain, their limitations as regards leading an autonomous life, their social support needs and other aspects concerning the effect of pain on their social and relational lives. This approach shows the importance of non-kin social support providers and the significant role of non-providers in the personal networks of people suffering chronic pain.
Flux-Independent NMDAR Signaling: Molecular Mediators, Cellular Functions, and Complexities
Authors: Montes de Oca Balderas Pavel
Published: November 2018

The glutamate (Glu) N-methyl-d-aspartate (NMDA) receptor (NMDAR) plays a critical role in synaptic communication given mainly by its ionotropic function that permeates Ca2+. This in turn activates calmodulin that triggers CaMKII, MAPK, CREB, and PI3K pathways, among others. However, NMDAR signaling is more complex. In the last two decades several groups have shown that the NMDAR also elicits flux-independent signaling (f-iNMDARs). It has been demonstrated that agonist (Glu or NMDA) or co-agonist (Glycine or d-Serine) bindings initiate intracellular events, including conformational changes, exchange of molecular interactions, release of second messengers, and signal transduction, that result in different cellular events including endocytosis, LTD, cell death, and neuroprotection, among others. Interestingly, f-iNMDARs has also been observed in pathological conditions and non-neuronal cells. Here, the molecular and cellular events elicited by these flux-independent actions (non-canonical or metabotropic-like) of the NMDAR are reviewed. Considering the NMDAR complexity, it is possible that these flux-independent events have a more relevant role in intracellular signaling that has been disregarded for decades. Moreover, considering the wide expression and function of the NMDAR in non-neuronal cells and other tissues beyond the nervous system and some evolutionary traits, f-iNMDARs calls for a reconsideration of how we understand the biology of this complex receptor.
Development of Spermatogenesis In Vitro in Three-Dimensional Culture from Spermatogonial Cells of Busulfan-Treated Immature Mice
Authors: AbuMadighem Ali , Solomon Ronnie , Stepanovsky Alina , Kapelushnik Joseph , Shi QingHua , Meese Eckart , Lunenfeld Eitan , Huleihel Mahmoud
Published: November 2018

Aggressive chemotherapy may lead to permanent male infertility. Prepubertal males do not generate sperm, but their testes do contain spermatogonial cells (SPGCs) that could be used for fertility preservation. In the present study, we examined the effect of busulfan (BU) on the SPGCs of immature mice, and the possible induction of the survivor SPGCs to develop spermatogenesis in 3D in-vitro culture. Immature mice were injected with BU, and after 0.5–12 weeks, their testes were weighed and evaluated histologically compared to the control mice. The spermatogonial cells [Sal-like protein 4 (SALL4) and VASA (a member of the DEAD box protein family) in the testicular tissue were counted/seminiferous tubule (ST). The cells from the STs were enzymatically isolated and cultured in vitro. Our results showed a significant decrease in the testicular weight of the BU-treated mice compared to the control. This was in parallel to a significant increase in the number of severely damaged STs, and a decrease in the number of SALL4 and VASA/STs compared to the control. The cultures of the isolated cells from the STs of the BU-treated mice showed a development of colonies and meiotic and post-meiotic cells after four weeks of culture. The addition of homogenates from adult GFP mice to those cultures induced the development of sperm-like cells after four weeks of culture. This is the first study demonstrating the presence of biologically active spermatogonial cells in the testicular tissue of BU-treated immature mice, and their capacity to develop sperm-like cells in vitro.
Regulation of iNOS on Immune Cells and Its Role in Diseases
Authors: Xue Qingjie , Yan Yingchun , Zhang Ruihua , Xiong Huabao
Published: November 2018

In recent years, there have been many studies on the function of nitric oxide synthase (NOS) in experimental animals and humans. This review analyzes and explores the relationship between inducible nitric oxide synthase (iNOS) and T cells, macrophages, and dendritic cell et al. differentiation using data based on laboratory research, highlighting recent NOS laboratory research. Our insights into research prospects and directions are also presented.
Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential in Brachypodium Distachyon Callus Culture
Authors: Betekhtin Alexander , Rojek Magdalena , Nowak Katarzyna , Pinski Artur , Milewska-Hendel Anna , Kurczynska Ewa , Doonan John H. , Hasterok Robert
Published: November 2018

Effective regeneration of callus tissue into embryos and then into whole plants is essential for plant biotechnology. The embryonic potential is often low and can further decrease with time in culture, which limits the utilisation of calli for transformation procedures and in vitro propagation. In this study, we show that the loss of embryogenic potential in callus cultures of Brachypodium distachyon is progressive over time. Flow cytometry analyses indicated endoploidy levels increased in 60- and 90-day-old calli with effective loss of the 2C DNA content peak in the latter. Analysis of indolic compounds content revealed a decrease in 60- and 90-day-old calli compared to either freshly isolated explants or 30-day-old calli. Immunohistochemical analysis revealed a decrease in arabinogalactan proteins (AGP) signal with the time of culture, but extensin (EXT) epitopes either increased (JIM12 epitopes) or decreased (JIM11 epitopes). The transcript accumulation levels of AGPs and EXTs confirmed these results, with most of AGP and EXT transcripts gradually decreasing. Some chimeric EXT transcripts significantly increased on the 30th day of culture, perhaps because of an increased embryogenic potential. Selected somatic embryogenesis-related genes and cyclins demonstrated a gradual decrease of transcript accumulation for YUCCA (YUC), AINTEGUMENTA-LIKE (AIL), BABY BOOM (BBM), and CLAVATA (CLV3) genes, as well as for most of the cyclins, starting from the 30th day of culture. Notably, WUSCHEL (WUS) transcript was detectable only on the 30th and 60th day and was not detectable in the zygotic embryos and in 90-day-old calli.
AMPK: Regulation of Metabolic Dynamics in the Context of Autophagy
Authors: Tamargo-Gómez Isaac , Mariño Guillermo
Published: November 2018

Eukaryotic cells have developed mechanisms that allow them to link growth and proliferation to the availability of energy and biomolecules. AMPK (adenosine monophosphate-activated protein kinase) is one of the most important molecular energy sensors in eukaryotic cells. AMPK activity is able to control a wide variety of metabolic processes connecting cellular metabolism with energy availability. Autophagy is an evolutionarily conserved catabolic pathway whose activity provides energy and basic building blocks for the synthesis of new biomolecules. Given the importance of autophagic degradation for energy production in situations of nutrient scarcity, it seems logical that eukaryotic cells have developed multiple molecular links between AMPK signaling and autophagy regulation. In this review, we will discuss the importance of AMPK activity for diverse aspects of cellular metabolism, and how AMPK modulates autophagic degradation and adapts it to cellular energetic status. We will explain how AMPK-mediated signaling is mechanistically involved in autophagy regulation both through specific phosphorylation of autophagy-relevant proteins or by indirectly impacting in the activity of additional autophagy regulators.
A Label-Free Fluorescent DNA Machine for Sensitive Cyclic Amplification Detection of ATP
Authors: Zhang Jingjing , Han Jialun , Feng Shehong , Niu Chaoqun , Liu Chen , Du Jie , Chen Yong
Published: November 2018

In this study, a target recycled amplification, background signal suppression, label-free fluorescent, enzyme-free deoxyribonucleic acid (DNA) machine was developed for the detection of adenosine triphosphate (ATP) in human urine. ATP and DNA fuel strands (FS) were found to trigger the operation of the DNA machine and lead to the cyclic multiplexing of ATP and the release of single stranded (SS) DNA. Double-stranded DNA (dsDNA) was formed on graphene oxide (GO) from the combination of SS DNA and complementary strands (CS′). These double strands then detached from the surface of the GO and in the process interacted with PicoGreen dye resulting in amplifying fluorescence intensity. The results revealed that the detection range of the DNA machine is from 100 to 600 nM (R2 = 0.99108) with a limit of detection (LOD) of 127.9 pM. A DNA machine circuit and AND-NOT-AND-OR logic gates were successfully constructed, and the strategy was used to detect ATP in human urine. With the advantage of target recycling amplification and GO suppressing background signal without fluorescent label and enzyme, this developed strategy has great potential for sensitive detection of different proteins and small molecules.
Improved Mechanical Properties and Energy Absorption of BCC Lattice Structures with Triply Periodic Minimal Surfaces Fabricated by SLM
Authors: Zhao Miao , Liu Fei , Fu Guang , Zhang David Z. , Zhang Tao , Zhou Hailun
Published: November 2018

The triply periodic minimal surface (TPMS) method is a novel approach for lattice design in a range of fields, such as impact protection and structural lightweighting. In this paper, we used the TPMS formula to rapidly and accurately generate the most common lattice structure, named the body centered cubic (BCC) structure, with certain volume fractions. TPMS-based and computer aided design (CAD) based BCC lattice structures with volume fractions in the range of 10–30% were fabricated by selective laser melting (SLM) technology with Ti–6Al–4V and subjected to compressive tests. The results demonstrated that local geometric features changed the volume and stress distributions, revealing that the TPMS-based samples were superior to the CAD-based ones, with elastic modulus, yield strength and compression strength increasing in the ranges of 18.9–42.2%, 19.2–29.5%, and 2–36.6%, respectively. The failure mechanism of the TPMS-based samples with a high volume fraction changed to brittle failure observed by scanning electron microscope (SEM), as their struts were more affected by the axial force and fractured on struts. It was also found that the TPMS-based samples have a favorable capacity to absorb energy, particularly with a 30% volume fraction, the energy absorbed up to 50% strain was approximately three times higher than that of the CAD-based sample with an equal volume fraction. Furthermore, the theoretic Gibson–Ashby mode was established in order to predict and design the mechanical properties of the lattice structures. In summary, these results can be used to rapidly create BCC lattice structures with superior compressive properties for engineering applications.
Green Procedure to Manufacture Nanoparticle-Decorated Paper Substrates
Authors: Schlemmer Werner , Fischer Wolfgang , Zankel Armin , Vukušić Tomislava , Filipič Gregor , Jurov Andrea , Blažeka Damjan , Goessler Walter , Bauer Wolfgang , Spirk Stefan , Krstulović Nikša
Published: November 2018

For this study, a paper impregnated with silver nanoparticles (AgNPs) was prepared. To prepare the substrates, aqueous suspensions of pulp fines, a side product from the paper production, were mixed with AgNP suspensions. The nanoparticle (NP) synthesis was then carried out via laser ablation of pure Ag in water. After the sheet formation process, the leaching of the AgNPs was determined to be low while the sheets exhibited antimicrobial activity toward Escherichia coli (E. coli).
Development and Characterization of Mechanically Durable Silicone-Polythiourethane Composites Modified with Tetrapodal Shaped ZnO Particles for the Potential Application as Fouling-Release Coating in the Marine Sector
Authors: Qiu Haoyi , Hölken Iris , Gapeeva Anna , Filiz Volkan , Adelung Rainer , Baum Martina
Published: November 2018

Ecological considerations strongly necessitate the development of environmentally friendly antifouling paints. A promising alternative to biocide containing antifouling paints are fouling-release coatings, which are non-toxic and designed to prevent permanent attachment of marine organisms to the surface, due to their low surface energy. However, these coatings suffer from insufficient mechanical properties, which make them unsuitable for mechanically stressed surfaces e.g., on ship hulls. To overcome those obstacles, polydimethylsiloxane (PDMS)-polythiourethane (PTU) composites modified with tetrapodal shaped micro-nano ZnO particles (t-ZnO) were produced and characterized by evaluating the surface energy, mechanical properties, and fouling-release performance. Among all variations, PTU/1 wt.% PDMS composites with 1 wt.% t-ZnO particles possess superior properties for applications as fouling-release coatings for maritime purposes.
A Computational Model for Drug Release from PLGA Implant
Authors: Milosevic Miljan , Stojanovic Dusica , Simic Vladimir , Milicevic Bogdan , Radisavljevic Andjela , Uskokovic Petar , Kojic Milos
Published: November 2018

Due to the relative ease of producing nanofibers with a core–shell structure, emulsion electrospinning has been investigated intensively in making nanofibrous drug delivery systems for controlled and sustained release. Predictions of drug release rates from the poly (d,l-lactic-co-glycolic acid) (PLGA) produced via emulsion electrospinning can be a very difficult task due to the complexity of the system. A computational finite element methodology was used to calculate the diffusion mass transport of Rhodamine B (fluorescent drug model). Degradation effects and hydrophobicity (partitioning phenomenon) at the fiber/surrounding interface were included in the models. The results are validated by experiments where electrospun PLGA nanofiber mats with different contents were used. A new approach to three-dimensional (3D) modeling of nanofibers is presented in this work. The authors have introduced two original models for diffusive drug release from nanofibers to the 3D surrounding medium discretized by continuum 3D finite elements: (1) A model with simple radial one-dimensional (1D) finite elements, and (2) a model consisting of composite smeared finite elements (CSFEs). Numerical solutions, compared to experiments, demonstrate that both computational models provide accurate predictions of the diffusion process and can therefore serve as efficient tools for describing transport inside a polymer fiber network and drug release to the surrounding porous medium.
Temperature-Dependent Domain Dynamics and Electrical Properties of Nd-doped Bi4Ti2.99Mn0.01O12 Thin Films in Fatigue Process
Authors: Zhang Wanli , Mao Yanhu , Yan Shaoan , Xiao Yongguang , Tang Minghua , Li Gang , Peng Qiangxiang , Li Zheng
Published: November 2018

Bi4Ti2.99Mn0.01O12 (BTM) thin films with different ratio of neodymium (Nd) doping were prepared on Pt(111)/Ti/SiO2/Si(100) substrates through a sol-gel method. The effects of Nd doping on domain dynamics and temperature-dependent fatigue behaviors of BTM thin films were systematically studied. The polarization fatigues of BTM (not doped) and Bi3.5Nd0.5Ti2.99Mn0.01O12 (BNTM05) thin films first get better with the increasing temperature (T) from 300 to 350 K and then become worse from 350 to 400 K, while Bi3.15Nd0.85Ti2.99Mn0.01O12 (BNTM85) thin films show enhanced fatigue endurance from 300 to 400 K. It can be shown that the long-range diffusion of oxygen vacancies in BTM thin film happens more easily through the impedance spectra analysis with T from 300 to 475 K, which can be verified by a lower activation energies (0.13–0.14 eV) compared to those of BNTM05 and BNTM85 (0.17–0.21 eV). Using a temperature-dependent piezoresponse force microscopy (PFM), we have found more responsive domain fragments in Nd-substituted films. The microscopic domain evolution from 298 to 448 K was done to further explain that the domain wall unpinning effect has been enhanced with increasing T. The correlation between microscopic domain dynamics and macroscopic electrical properties clearly demonstrates the effects of charged domain wall in Nd-doped BTM thin films during the fatigue tests.
Microstructure, Texture, Electrical and Mechanical Properties of AA-6063 Processed by Multi Directional Forging
Authors: Dashti Alireza , Shaeri Mohammad Hossein , Taghiabadi Reza , Djavanroodi Faramarz , Vali Ghazvini Farzaneh , Javadi Hamid
Published: November 2018

In current research, the effect of the multi-directional forging (MDF) process on the microstructure, texture, mechanical and electrical properties of AA-6063 under different heat treatment conditions at various MDF temperatures was studied. The annealed AA-6063 alloy was processed up to three passes of MDF at ambient temperature. Three passes of this process were also applied to the solution-treated AA-6063 at ambient temperature and 177 °C. Microstructural investigations demonstrated that the MDF process led to a significant reduction in the average grain size as well as a considerable increase in the fraction of low angle grain boundaries. Texture analysis revealed that copper and Goss textures were mainly developed within the annealed and solution-treated samples of AA-6063, respectively. The hardness and shear strength values of all processed samples also showed a sizeable improvement compared to the initial heat-treated samples. For example, the hardness and shear yield strength value of the solution-treated sample MDFed for three passes showed more than 100 and 70% increase, respectively. The effect of the MDF process on the electrical conductivity of AA-6063 under different heat treatment conditions at various temperatures was negligible. So, it can be concluded that the MDF process increased the mechanical properties without an appreciable decrease in electrical conductivity.
Determination of the Elasticity Modulus of 3D-Printed Octet-Truss Structures for Use in Porous Prosthesis Implants
Authors: Bagheri Ali , Buj-Corral Irene , Ferrer Ballester Miquel , Magdalena Pastor Maria , Roure Fernandez Francesc
Published: November 2018

In tissue engineering, scaffolds can be obtained by means of 3D printing. Different structures are used in order to reduce the stiffness of the solid material. The present article analyzes the mechanical behavior of octet-truss microstructures. Three different octet structures with strut radii of 0.4, 0.5, and 0.6 mm were studied. The theoretical relative densities corresponding to these structures were 34.7%, 48.3%, and 61.8%, respectively. Two different values for the ratio of height (H) to width (W) were considered, H/W = 2 and H/W = 4. Several specimens of each structure were printed, which had the shape of a square base prism. Compression tests were performed and the elasticity modulus (E) of the octet-truss lattice-structured material was determined, both, experimentally and by means of Finite Element Methods (FEM). The greater the strut radius, the higher the modulus of elasticity and the compressive strength. Better agreement was found between the experimental and the simulated modulus of elasticity results for H/W = 4 than for H/W = 2. The octet-truss lattice can be considered to be a promising structure for printing in the field of tissue engineering.
Influence of Gradual Damage on the Structural Dynamic Behaviour of Composite Rotors: Experimental Investigations
Authors: Filippatos Angelos , Gude Maik
Published: November 2018

Fibre-reinforced composite structures subjected to complex loads exhibit gradual damage behaviour with the degradation of the effective mechanical properties and changes in their structural dynamic behaviour. Damage manifests itself as a spatial increase in inter-fibre failure and delamination growth, resulting in local changes in stiffness. These changes affect not only the residual strength but, more importantly, the structural dynamic behaviour. In the case of composite rotors, this can lead to catastrophic failure if an eigenfrequency coincides with the rotational speed. The description and analysis of the gradual damage behaviour of composite rotors, therefore, provide the fundamentals for a better understanding of unpredicted structural phenomena. The gradual damage behaviour of the example composite rotors and the resulting damage-dependent dynamic behaviour were experimentally investigated under propagating damage caused by a combination of out-of-plane and in-plane loads. A novel observation is the finding that a monotonic increase in damage results in a non-monotonic frequency shift of a significant number of eigenfrequencies.
Preparation of the Sodium Alginate-g-(Polyacrylic Acid-co-Allyltrimethylammonium Chloride) Polyampholytic Superabsorbent Polymer and Its Dye Adsorption Property
Authors: Tang Shuxian , Zhao Ying , Wang Haitao , Wang Yuqiao , Zhu Hexiang , Chen Yu , Chen Shusen , Jin Shaohua , Yang Ziming , Li Puwang , Li Sidong
Published: November 2018

A polyampholytic superabsorbent polymer (PASAP), sodium alginate-g-(polyacrylic acid-co-allyltrimethylammonium chloride) (SA-g-(PAA-co-PTM)), was prepared by free-radical graft copolymerization and characterized. The polymer exhibited pH-dependent swelling behaviors with extremely high swelling ratios, and was saline tolerant. The dye adsorption properties of SA-g-(PAA-co-PTM) were investigated using methylene blue (MB) as a cationic dye model. It was found that its dye adsorption capacity was significantly affected by the TM content in PASAP and pH of dye solution. The dye adsorption kinetics and isotherm obey the pseudo-second-order kinetic model and the Langmuir isotherm model, respectively, and the adsorption process is chemisorption in nature. In addition, SA-g-(PAA-co-PTM) exhibited high MB adsorption capacities in a wide pH range and reusability in at least five adsorption-desorption cycles, indicating its great application potentials as the adsorbent for dye removals from effluents.
Modeling of Gate Stack Patterning for Advanced Technology Nodes: A Review
Authors: Klemenschits Xaver , Selberherr Siegfried , Filipovic Lado
Published: November 2018

Semiconductor device dimensions have been decreasing steadily over the past several decades, generating the need to overcome fundamental limitations of both the materials they are made of and the fabrication techniques used to build them. Modern metal gates are no longer a simple polysilicon layer, but rather consist of a stack of several different materials, often requiring multiple processing steps each, to obtain the characteristics needed for stable operation. In order to better understand the underlying mechanics and predict the potential of new methods and materials, technology computer aided design has become increasingly important. This review will discuss the fundamental methods, used to describe expected topology changes, and their respective benefits and limitations. In particular, common techniques used for effective modeling of the transport of molecular entities using numerical particle ray tracing in the feature scale region will be reviewed, taking into account the limitations they impose on chemical modeling. The modeling of surface chemistries and recent advances therein, which have enabled the identification of dominant etch mechanisms and the development of sophisticated chemical models, is further presented. Finally, recent advances in the modeling of gate stack pattering using advanced geometries in the feature scale are discussed, taking note of the underlying methods and their limitations, which still need to be overcome and are actively investigated.
Identification, Characterization and Quantification of Process-Related and Degradation Impurities in Lisdexamfetamine Dimesylate: Identifiction of Two New Compounds
Authors: Gao Shenghua , Meng Lili , Zhao Chunjie , Zhang Tao , Qiu Pengcheng , Zhang Fuli
Published: November 2018

Twelve impurities (process-related and degradation) in lisdexamfetamine dimesylate (LDX), a central nervous system (CNS) stimulant drug, were first separated and quantified by high-performance liquid chromatography (HPLC) and then identified by liquid chromatography mass spectrometry (LC-MS). The structures of the twelve impurities were further confirmed and characterized by IR, HRMS and NMR analyses. Based on the characterization data, two previously unknown impurities formed during the process development and forced degradation were proposed to be (2S)-2,6-di-(lysyl)-amino-N-[(1S)-1-methyl-2-phenyl ethyl]hexanamide (Imp-H) and (2S)-2,6-diamino-N-[(1S)-1-methyl-2-(2-hydroxyphenyl)ethyl] hexanamide (Imp-M). Furthermore, these two compounds are new. Probable mechanisms for the formation of the twelve impurities were discussed based on the synthesis route of LDX. Superior separation was achieved on a YMC-Pack ODS-AQ S5 120A silica column (250 × 4.6 mm × 5 μm) using a gradient of a mixture of acetonitrile and 0.1% aqueous methanesulfonic acid solution. The HPLC method was optimized in order to separate, selectively detect, and quantify all the impurities. The full identification and characterization of these impurities should prove useful for quality control in the manufacture of lisdexamfetamine dimesylate.
Design, Synthesis, and Biological Activity of Novel Myricetin Derivatives Containing Amide, Thioether, and 1,3,4-Thiadiazole Moieties
Authors: Ruan Xianghui , Zhang Cheng , Jiang Shichun , Guo Tao , Xia Rongjiao , Chen Ying , Tang Xu , Xue Wei
Published: November 2018

A series of myricetin derivatives containing amide, thioether, and 1,3,4-thiadiazole moieties were designed and synthesized, and their antiviral and antibacterial activities were assessed. The bioassays showed that all the title compounds exhibited potent in vitro antibacterial activities against Xanthomonas citri (Xac), Ralstonia solanacearum (Rs), and Xanthomonas oryzae pv. Oryzae (Xoo). In particular, the compounds 5a, 5f, 5g, 5h, 5i, and 5l, with EC50 values of 11.5–27.3 μg/mL, showed potent antibacterial activity against Xac that was better than the commercial bactericides Bismerthiazol (34.7 μg/mL) and Thiodiazole copper (41.1% μg/mL). Moreover, the in vivo antiviral activities against tobacco mosaic virus (TMV) of the target compounds were also tested. Among these compounds, the curative, protection, and inactivation activities of 5g were 49.9, 52.9, and 73.3%, respectively, which were better than that of the commercial antiviral Ribavirin (40.6, 51.1, and 71.1%, respectively). This study demonstrates that myricetin derivatives bearing amide, thioether, and 1,3,4-thiadiazole moieties can serve as potential alternative templates for the development of novel, highly efficient inhibitors against plant pathogenic bacteria and viruses.
Biodistribution and Tumor Uptake of 67Ga-Nimotuzumab in a Malignant Pleural Mesothelioma Xenograft
Authors: Izquierdo-Sánchez Vanessa , Muñiz-Hernández Saé , Vázquez-Becerra Héctor , Pacheco-Yepez Judith , Romero-Piña Mario E. , Arrieta Oscar , Medina Luis Alberto
Published: November 2018

Malignant pleural mesothelioma (MPM) is the most common tumor of the pulmonary pleura. It is a rare and aggressive malignancy, generally associated with continuous occupational exposure to asbestos. Only a multimodal-approach to treatment, based on surgical resection, chemotherapy and/or radiation, has shown some benefits. However, the survival rate remains low. Nimotuzumab (h-R3), an anti-EGFR (epidermal growth factor receptor) humanized antibody, is proposed as a promising agent for the treatment of MPM. The aim of this research was to implement a procedure for nimotuzumab radiolabeling to evaluate its biodistribution and affinity for EGF (epidermal growth factor) receptors present in a mesothelioma xenograft. Nimotuzumab was radiolabeled with 67Ga; radiolabel efficiency, radiochemical purity, serum stability, and biodistribution were evaluated. Biodistribution and tumor uptake imaging studies by microSPECT/CT in mesothelioma xenografts revealed constant nimotuzumab uptake at the tumor site during the first 48 h after drug administration. In vivo studies using MPM xenografts showed a significant uptake of this radioimmunoconjugate, which illustrates its potential as a biomarker that could promote its theranostic use in patients with MPM.
Inhibitory Effects of 2N1HIA (2-(3-(2-Fluoro-4-Methoxyphenyl)-6-Oxo-1(6H)-Pyridazinyl)-N-1H-Indol-5-Ylacetamide) on Osteoclast Differentiation via Suppressing Cathepsin K Expression
Authors: Ahn Sun-Hee , Chen Zhihao , Lee Jinkyung , Lee Seok-Woo , Min Sang Hyun , Kim Nam Doo , Lee Tae-Hoon
Published: November 2018

Osteoclasts are large multinucleated cells which are induced by the regulation of the receptor activator of nuclear factor kappa-Β ligand (RANKL), which is important in bone resorption. Excessive osteoclast differentiation can cause pathologic bone loss and destruction. Numerous studies have targeted molecules inhibiting RANKL signaling or bone resorption activity. In this study, 11 compounds from commercial libraries were examined for their effect on RANKL-induced osteoclast differentiation. Of these compounds, only 2-(3-(2-fluoro-4-methoxyphenyl)-6-oxo-1(6H)-pyridazinyl)-N-1H-indol-5-ylacetamide (2N1HIA) caused a significant decrease in multinucleated tartrate-resistant acid phosphatase (TRAP)-positive cell formation in a dose-dependent manner, without inducing cytotoxicity. The 2N1HIA compound neither affected the expression of osteoclast-specific gene markers such as TRAF6, NFATc1, RANK, OC-STAMP, and DC-STAMP, nor the RANKL signaling pathways, including p38, ERK, JNK, and NF-κB. However, 2N1HIA exhibited a significant impact on the expression levels of CD47 and cathepsin K, the early fusion marker and critical protease for bone resorption, respectively. The activity of matrix metalloprotease-9 (MMP-9) decreased due to 2N1HIA treatment. Accordingly, bone resorption activity and actin ring formation decreased in the presence of 2N1HIA. Taken together, 2N1HIA acts as an inhibitor of osteoclast differentiation by attenuating bone resorption activity and may serve as a potential candidate in preventing and/or treating osteoporosis, or other bone diseases associated with excessive bone resorption.
O-Aminoalkyl-O-Trimethyl-2,3-Dehydrosilybins: Synthesis and In Vitro Effects Towards Prostate Cancer Cells
Authors: Vue Bao , Zhang Sheng , Vignau Andre , Chen Guanglin , Zhang Xiaojie , Diaz William , Zhang Qiang , Zheng Shilong , Wang Guangdi , Chen Qiao-Hong
Published: November 2018

As part of our ongoing silybin project, this study aims to introduce a basic nitrogen-containing group to 7-OH of 3,5,20-O-trimethyl-2,3-dehydrosilybin or 3-OH of 5,7,20-O-trimethyl-2,3-dehydrosilybin via an appropriate linker for in vitro evaluation as potential anti-prostate cancer agents. The synthetic approaches to 7-O-substituted-3,5,20-O-trimethyl-2,3-dehydrosilybins through a five-step procedure and to 3-O-substituted-5,7,20-O-trimethyl-2,3- dehydrosilybins via a four-step transformation have been developed. Thirty-two nitrogen-containing derivatives of silybin have been achieved through these synthetic methods for the evaluation of their antiproliferative activities towards both androgen-sensitive (LNCaP) and androgen-insensitive prostate cancer cell lines (PC-3 and DU145) using the WST-1 cell proliferation assay. These derivatives exhibited greater in vitro antiproliferative potency than silibinin. Among them, 11, 29, 31, 37, and 40 were identified as five optimal derivatives with IC50 values in the range of 1.40–3.06 µM, representing a 17- to 52-fold improvement in potency compared to silibinin. All these five optimal derivatives can arrest the PC-3 cell cycle in the G0/G1 phase and promote PC-3 cell apoptosis. Derivatives 11, 37, and 40 are more effective than 29 and 31 in activating PC-3 cell apoptosis.
Characterization of Polystyrene Wastes as Potential Extruded Feedstock Filament for 3D Printing
Authors: Turku Irina , Kasala Sushil , Kärki Timo
Published: November 2018

The recyclability of polystyrene, acrylonitrile butadiene styrene and polyvinylchloride waste and their use as a source for 3D printing were studied. Filaments of about 3 mm in diameter were extruded successfully with a small-size extruder. The processed filaments were tested on a broad range of parameters-melt flow index, glass transition temperature, tensile properties and a pyrolysis scenario were obtained. The measured parameters were compared with parameters of virgin counterparts presented in the literature. In order to estimate the composition of the recycled material, Fourier Transform Infrared and elemental analysis of the samples was done.
Agronomic and Economic Potential of Vegetation Indices for Rice N Recommendations under Organic and Mineral Fertilization in Mediterranean Regions
Authors: Moreno-García Beatriz , Casterad Mª Auxiliadora , Guillén Mónica , Quílez Dolores
Published: November 2018

Rice (Oryza sativa L.) farmers in Mediterranean regions usually apply organic or mineral fertilizers before seeding that are supplemented with mineral nitrogen (N) later in the season. In general, the midseason N is applied without consideration of the actual crop N status, which may lead to over-fertilization and associated environmental problems. Thus, the purpose of this study was to design and evaluate a N recommendation approach using aerial images for Mediterranean paddy rice systems. A two-year rice field experiment was established in northeastern Spain, with different rates of pig slurry (PS) and mineral N fertilizer. Multispectral aerial images were taken at the rice booting stage, and several vegetation indices (VIs) were calculated. The VIs showed strong relationships with yield and the relations significantly differed between the PS and mineral fertilization treatments. The strongest relations with yield were obtained with gMCARINIR, proposed in this study, (R2 = 0.67), GNDVI (R2 = 0.64) and MCARINIR (R2 = 0.64), indicating the importance of including the green band information. The N recommendation approach generated using the VIs information showed a high success (87.5%) in the preliminary evaluation. The economic and environmental analysis showed that this approach provides a useful tool when compared to the usual farmer practices.
Ice Surface Temperature Retrieval from a Single Satellite Imager Band
Authors: Liu Yinghui , Dworak Richard , Key Jeffrey
Published: November 2018

Current methods for estimating the surface temperature of sea and lake ice—the ice surface temperature (IST)—utilize two satellite imager thermal bands (11 and 12 μm) at moderate spatial resolution. These “split-window” or dual-band methods have been shown to have low biases and uncertainties. A single-band algorithm would be useful for satellite imagers that have only the 11 μm band at high resolution, such as the Visible Infrared Imaging Radiometer Suite (VIIRS), or that do not have a fully functional 12 μm band, such as the Thermal Infrared Sensor onboard the Landsat 8. This study presents a method for single-band IST retrievals, and validation of the retrievals using IST measurements from an airborne infrared radiation pyrometer during the NASA IceBridge campaign in the Arctic. Results show that IST with a single thermal band from the VIIRS has comparable performance to IST with the VIIRS dual-band (split-window) method, with a bias of 0.22 K and root-mean-square error of 1.03 K.
Freshwater Fish Habitat Complexity Mapping Using Above and Underwater Structure-From-Motion Photogrammetry
Authors: Kalacska Margaret , Lucanus Oliver , Sousa Leandro , Vieira Thiago , Arroyo-Mora Juan Pablo
Published: November 2018

Substrate complexity is strongly related to biodiversity in aquatic habitats. We illustrate a novel framework, based on Structure-from-Motion photogrammetry (SfM) and Multi-View Stereo (MVS) photogrammetry, to quantify habitat complexity in freshwater ecosystems from Unmanned Aerial Vehicle (UAV) and underwater photography. We analysed sites in the Xingu river basin, Brazil, to reconstruct the 3D structure of the substrate and identify and map habitat classes important for maintaining fish assemblage biodiversity. From the digital models we calculated habitat complexity metrics including rugosity, slope and 3D fractal dimension. The UAV based SfM-MVS products were generated at a ground sampling distance (GSD) of 1.20–2.38 cm while the underwater photography produced a GSD of 1 mm. Our results show how these products provide spatially explicit complexity metrics, which are more comprehensive than conventional arbitrary cross sections. Shallow neural network classification of SfM-MVS products of substrate exposed in the dry season resulted in high accuracies across classes. UAV and underwater SfM-MVS is robust for quantifying freshwater habitat classes and complexity and should be chosen whenever possible over conventional methods (e.g., chain-and-tape) because of the repeatability, scalability and multi-dimensional nature of the products. The SfM-MVS products can be used to identify high priority freshwater sectors for conservation, species occurrences and diversity studies to provide a broader indication for overall fish species diversity and provide repeatability for monitoring change over time.
Glacial Lakes in the Nepal Himalaya: Inventory and Decadal Dynamics (1977–2017)
Authors: Khadka Nitesh , Zhang Guoqing , Thakuri Sudeep
Published: November 2018

Himalayan glaciers, in general, are shrinking and glacial lakes are evolving and growing rapidly in number and size as a result of climate change. This study presents the latest remote sensing-based inventory (2017) of glacial lakes (size ≥0.0036 km2) across the Nepal Himalaya using optical satellite data. Furthermore, this study traces the decadal glacial lake dynamics from 1977 to 2017 in the Nepal Himalaya. The decadal mapping of glacial lakes (both glacial-fed and nonglacial-fed) across the Nepal Himalaya reveals an increase in the number and area of lakes from 1977 to 2017, with 606 (55.53 ± 16.52 km2), 1137 (64.56 ± 11.64 km2), 1228 (68.87 ± 12.18 km2), 1489 (74.2 ± 14.22 km2), and 1541 (80.95 ± 15.25 km2) glacial lakes being mapped in 1977, 1987, 1997, 2007, and 2017, respectively. Glacial lakes show heterogeneous rates of expansion in different river basins and elevation zones of Nepal, with apparent decadal emergences and disappearances. Overall, the glacial lakes exhibited ~25% expansion of surface areas from 1987 to 2017. For the period from 1987 to 2017, proglacial lakes with ice contact, among others, exhibited the highest incremental changes in terms of number (181%) and surface area (82%). The continuous amplified mass loss of glaciers, as reported in Central Himalaya, is expected to accompany glacial lake expansion in the future, increasing the risk of glacial lake outburst floods (GLOFs). We emphasize that the rapidly increasing glacial lakes in the Nepal Himalaya can pose potential GLOF threats to downstream population and infrastructure.
Assessment of Satellite-Derived Precipitation Products for the Beijing Region
Authors: Ren Meifang , Xu Zongxue , Pang Bo , Liu Wenfeng , Liu Jiangtao , Du Longgang , Wang Rong
Published: November 2018

Performance of four satellite precipitation products, namely, the China Meteorological Forcing Dataset (CMFD), Climate Prediction Center morphing technique (CMORPH), as well as 3B42 calibrated and 3B42-RT dataset, which are derived from the Tropical Rainfall Measuring Mission (TRMM) and Multi-satellite Precipitation Analysis (TMPA), were evaluated from daily to annual temporal scales over Beijing, using observations from 36 ground meteorological stations. Five statistical properties and three categorical metrics were used to test the results. The assessment showed that all four satellite precipitation products captured the temporal variability of precipitation. Although four satellite precipitation products captured the trend of more precipitation in the northeastern regions, all four products showed different distribution from the observations for 2001–2015 over Beijing. All precipitation products tended to overestimate moderate precipitation events and underestimate heavy precipitation events over Beijing, except for 3B42RT, which tended to overestimate most precipitation events. By comparison, the CMFD performed better than the CMORPH, 3B42 calibrated, and 3B42-RT datasets, having the higher correlation coefficient and low root mean squared difference, and mean absolute difference at all temporal scales. The average correlation coefficient of the CMFD, CMORPH, 3B42 calibrated, and 3B42-RT products for all 36 stations were 0.70, 0.60, 0.59, and 0.54 for daily precipitation and 0.78, 0.32, 0.74, and 0.44 for monthly precipitation. Overall, the CMFD was the most reliable for the Beijing region.