bluuebunny/crossref_metadata_2025 huggingface dataset:
Model of the “Economic Cross” of the Digital Platform: Opportunities and Conditions for Interindustry Technological Convergence
Authors: Davydova Natalya , Timokhin Dmitry , Trubacheev Evgeniy
Published: October 2024

The platform economy is an objective stage in the evolution of the digital economy, whose development is carried out under the influence of the trend of involving a large number of SMEs and individuals in economic relations as their direct participants. Unlike the pre-digital era, when operational commercial direct interaction between small participants in economic relations turned out to be impossible, communication technologies of the second decade of the 21st century have created favorable opportunities for the development of independent businesses of individuals and small professional teams. The process of commercialization of household resources is actively taking place in the form of the development of private blogs as platforms for commercial advertising and mutual trade of goods and services by individuals online, which creates a direct competitive threat to traditional trading companies. The current stage in the development of online participation of SMEs and individuals in commercial relations is the development of outsourcing relationships, within which part of the processes implemented by large businesses independently using hired personnel and their own infrastructure will be delegated to independent network structures on a competitive basis. At the same time, in proportion to the growing popularity of e-commerce, public demands for the security of such transactions are increasing, which is especially important in conditions of sanctions uncertainty and high risks of supply chain collapse. This circumstance determines the business request for systematization of the digital space and its centralization, the terminal stage of which is an inter-industry digital platform. The purpose of the scientific article is to develop a system of proposals for the formation of a national intersectoral outsourcing platform based on the “economic cross” model. A system of innovative solutions has been developed regarding the organization of economic interaction between subjects, the procedure for financing the activities of the platform by its participants, and the distribution of indirect industry income arising from the functioning of the platform.
Characterising
g
lycosaminoglycans in
h
uman
b
reastmilk and their
p
otential
r
ole in
i
nfant
health
Authors: Greenwood Melissa , Murciano-Martinez Patricia , Berrington Janet , Flitsch Sabine L , Austin Sean , Stewart Christopher
Published: October 2024

Human breastmilk is composed of many well researched bioactive components crucial for infant nutrition and priming of the neonatal microbiome and immune system. Understanding these components gives us crucial insight to the health and wellbeing of infants. Research surrounding glycosaminoglycans (GAGs) previously focused on those produced endogenously; however, recent efforts have shifted to understanding GAGs in human breastmilk. The structural complexity of GAGs makes detection and analysis complicated therefore, research is time consuming and limited to highly specialised teams experienced in carbohydrate analysis. In breastmilk, GAGs are present in varying quantities in four forms; chondroitin sulphate, heparin/heparan sulphate, dermatan sulphate and hyaluronic acid, and are hypothesised to behave similar to other bioactive components with suspected roles in pathogen defense and proliferation of beneficial gut bacteria. Chondroitin sulphate and heparin, being the most abundant, are expected to have the most impact on infant health. Their decreasing concentration over lactation further indicates their role and potential importance during early life.
The 'Histoire des Deux Indes'
Authors: Kwiatkowski Nicolás
Published: October 2024

This article proposes an interpretation of the Histoire philosophique et politique des établissements et du commerce des Européens dans les deux Indes (1770) within the intellectual context of Enlightenment historiography. The first section describes the book, its contents, editions, authorship, and censorship. The second section provides a critical synthesis of the history of historiography on the work. The third section proposes an interpretation of the Histoire as an enlightened critique of colonialism and empire. A brief conclusion situates the book within a specific definition of the Enlightenment that accounts for its contradictions rather than attempting to overcome them.
El pasado como puesta en juego
Authors: Napoli Diana
Published: October 2024

Este artículo explora las reflexiones de Certeau para mostrar cómo el pasado, dentro del contexto de la operación historiográfica, más que ser un referente estable para investigar, representa un resultado y una puesta en juego a través de la cual una sociedad, al transformar la muerte y la pérdida en objeto de conocimiento, permite un intercambio entre los vivos. Se plantean preocupaciones sobre una experiencia del tiempo centrada principalmente en el presente, que interpreta el pasado sobre todo desde un paradigma anticuario, y sobre un horizonte marcado por la amenaza inminente del cambio climático, en el que el futuro parece desaparecer. Dado este contexto, en el cual el valor de la historiografía como “tópica de lo inteligible” está fuertemente erosionado, se contemplan posibles formas de configurar el pasado, que, siendo capaces de asumir la pérdida, puedan abrir el horizonte del futuro.
Ortaokul Matematik Öğretmenlerinin Etkinlik Tercihlerinde Belirleyici Olan Dijital Materyal Özellikleri: Bir Durum Çalışması
Authors: Özbey Nuh , Özmantar Mehmet Fatih
Published: October 2024

Matematiksel etkinlikler üzerine yapılan araştırmalar, etkinliklerin seçiminde önemli bir rol oynayan öğretmenlerin, çeşitli olguları dikkate alarak etkinliklere titizlikle karar verdiklerini göstermektedir. Bu bağlamda gerçekleştirilen çalışmanın amacı, ortaokul matematik öğretmenlerinin, derslerinde kullanacakları etkinlikleri belirleme sürecinde, dijital materyallerin sahip olduğu özelliklerin, tercihlerini nasıl etkilediğini incelemektir. Araştırmada, yöntem olarak çoklu durum deseni kullanılmıştır. Çalışmanın katılımcıları, etkinlik temelli öğretim gerçekleştirmeye özen gösteren üç ortaokul matematik öğretmenidir. Veri toplama sürecinde katılımcılar ile kendi okullarında altı hafta boyunca yarı yapılandırılmış görüşmeler gerçekleştirilmiştir. Bu süreçte katılımcılardan, aynı kazanıma yönelik olarak Geogebra ile hazırlanan ve farklı özelliklerin kullanımını gerektiren etkinlikler arasından, derslerinde kullanma isteğine göre sıralama yapmaları ve bu tercihlerini gerekçelendirmeleri istenmiştir. Araştırma sürecinde elde edilen veriler, tematik içerik analizi yöntemiyle analiz edilmiştir. Bulgular, katılımcıların etkinlik tercihlerinde dijital materyallerin etkisine ilişkin düşüncelerinin yedi tema altında toplandığını göstermektedir. Bu temalar; dijital materyallerin; öğrencinin odaklanmasına etkisi, öğrencinin hazırbulunuşluk düzeyiyle uyumu, zaman yönetimine katkısı, öğrenme çıktılarına ulaşmadaki rolü, öğretim ilkelerine uygunluk derecesi, kullanım kolaylığı veya zorluğu ve ilgi çekicilik düzeyidir. Araştırma sonuçları, dijital materyal özelliklerinin, öğretmenlerin etkinlik seçimlerinde oldukça önemli bir faktör olduğunu ortaya koymaktadır. Bu kapsamda, içerik geliştiricilerine, dijital materyallerin öğretim ihtiyaçlarına uygunluğunu artırarak, öğretmenlerin etkinlik seçim süreçlerini desteklemeleri önerilmektedir.
Plumbagin Induces Apoptosis in Diffuse Large B-Cell Lymphoma by Modulating the ROS-PI3K-Akt-mTOR Signaling Pathway
Authors: Du Jingfang , Ye Tao , Li Pian , Yu Yanfang , Fan Fengli , Zhang Ruiying , Shen Na
Published: October 2024

This study aimed to investigate the potential effects and underlying mechanism of plumbagin (PL) on the proliferation and apoptosis of SU-DHL-4 cells, a type of diffuse large B-cell lymphoma (DLBCL), through in vitro and in vivo experiments. The in vitro experiments were performed by subjecting SU-DHL-4 cells to different concentrations of PL. The proliferation rate of the cells was evaluated using the CCK8 assay. Flow cytometry, quantitative real-time polymerase chain reaction (qRT-PCR), and a commercial ROS detection kit were employed to quantify the apoptosis rate, the antioxidant enzyme activity, and the levels of reactive oxygen species (ROS), respectively. The protein expression of Bax, BCL2, caspase-3, cleaved caspase-3, PI3K, p-PI3K, Akt, p-Akt, mTOR, and p-mTOR were determined by western blotting. The cell-derived tumor xenograft tumor model was constructed by subcutaneously injecting SU-DHL-4 cells into NOD-SCID mice. The therapeutic effect of PL was then evaluated by morphological staining. Results from the in vitro experiments demonstrated that PL could effectively inhibit cell proliferation, increase the production of reactive oxygen species (ROS), and induce apoptosis in SU-DHL-4 cells in both a time- and a dosage-dependent manner. Furthermore, PL treatment upregulated the protein expression of Bax and cleaved caspase-3 (P < 0.05). In parallel, PL treatment concurrently DOWNREGULATED the protein expression of Bcl-2, p-PI3K, p-Akt, and p-mTOR (P < 0.05). More important, it inhibits the growth of mouse xenograft tumors. PL promotes apoptosis of DLBCL cells, potentially by upregulating ROS and suppressing the PI3K/Akt/mTOR signaling pathway. These findings might be a useful reference for future drug discovery.
Exploring the Potential of Spent Pleurotus eryngii (Agaricomycetes) Substrate to Cultivate Pleurotus florida with Enhanced Productivity, Nutritional Profile, and Therapeutic Potential
Authors: Thakur Preeti , Jandaik Savita , Guleria Shikha , Sharma Annu , Sharma Ranjana , Singh Rajender
Published: October 2024

Every year, several mushroom farms create a significant volume of discarded mushroom substrate. To make use of spent mushroom substrate, Pleurotus florida was grown on wheat straw that had been supplemented in varying amounts with one year old spent substrate of Pleurotus eryngii, wheat bran, and CaCo3. Physico-chemical parameters of the substrates (pH, C, and N) were higher prior to cultivation and reduced following cultivation except electrical conductivity, that displayed the opposite trend. The wheat straw used as the control showed the highest yield, approximate composition, myco-chemicals, and antioxidant activity of P. florida sporophores. According to the data, the optimal ratio for P florida production following control, was for a combination of P. eryngii spent substrate (PESS) + wheat straw + wheat bran + CaCO3 (50:40:9.5:0.5). The results imply that adding a suitable quantity of wheat straw, wheat bran and CaCo3 to discarded mushroom substrate can enable its reuse, offering a practical method for preserving the environment, cutting expenses, and producing high-quality mushrooms.
Pattern design for capacity building of promoters in climate-smart agriculture environment
Authors: Rafiee Sefid Dashti Mohsen , Mirdamadi Seyed Mehdi , Farajollah Hosseini Seyed Jamal , Shokri ShahabAlddin
Published: October 2024

This study aimed to investigate the impact of agricultural environment, organisational, educational, economic, social, technical, cultural, and legal factors on the capacity building of extension experts in the development of climate smart agriculture. The research is applied in terms of its objective and descriptive survey in terms of its method. The results showed that infrastructural factors had the highest impact on climate-smart agriculture with 22%, followed by organisational factors with 21%, social factors with almost 17%, cultural factors with 16.5%, educational factors with 13%, legal factors with nearly 13%, technical factors with 11.4%, and, finally, economic factors with just over 10%. Overall, the factors under study explain more than 68% of the variance in climate-smart agriculture (R2 = 0.687), indicating the impact of the aforementioned factors on the development of climate-smart agriculture. Also, the design of this model made the promoters, by identifying and increasing the potential and capacities of themselves, villagers, and farmers, to be able to adapt themselves to climate change and drought and, by increasing resilience and adaptability, to have positive effects for the development of sustainable agriculture. Along with increasing the product, it will increase the income and reduce the costs for them.
Transmembrane Serine Protease 2 and Proteolytic Activation of the Epithelial Sodium Channel in Mouse Kidney
Authors: Sure Florian , Afonso Sara , Essigke Daniel , Schmidt Paul , Kalo M. Zaher , Nesterov Viatcheslav , Kißler Alicia , Bertog Marko , Rinke Ralf , Wittmann Sabine , Broeker Katharina A.E. , Gramberg Thomas , Artunc Ferruh , Korbmacher Christoph , Ilyaskin Alexandr V.
Published: October 2024

Key Points

Proteolytic activation of the epithelial sodium channel (ENaC) was compromised by transmembrane serine protease 2 deficiency in murine cortical collecting duct cells and native mouse kidney.

To compensate for impaired ENaC activation, rise in plasma aldosterone in response to low-salt diet was enhanced in Tmprss2
−/− mice.

Transmembrane serine protease 2 may be a potential drug target to limit proteolytic ENaC activation in disorders with increased renal ENaC activity.

Background

The renal epithelial sodium channel (ENaC) is essential for sodium balance and BP control. ENaC undergoes complex proteolytic activation by not yet clearly identified tubular proteases. Here, we examined a potential role of transmembrane serine protease 2 (TMPRSS2).

Methods

Murine ENaC and TMPRSS2 were (co)expressed in Xenopus laevis oocytes. ENaC cleavage and function were studied in TMPRSS2-deficient murine cortical collecting duct (mCCDcl1) cells and TMPRSS2-knockout (Tmprss2

−/−
) mice. Short-circuit currents (I
SC) were measured to assess ENaC-mediated transepithelial sodium transport of mCCDcl1 cells. The mCCDcl1 cell transcriptome was studied using RNA sequencing. The effect of low-sodium diet with or without high potassium were compared in Tmprss2

−/−
and wild-type mice using metabolic cages. ENaC-mediated whole-cell currents were recorded from microdissected tubules of Tmprss2

−/−
and wild-type mice.

Results

In oocytes, coexpression of murine TMPRSS2 and ENaC resulted in fully cleaved γ-ENaC and approximately two-fold stimulation of ENaC currents. High baseline expression of TMPRSS2 was detected in mCCDcl1 cells without a stimulatory effect of aldosterone on its function or transcription. TMPRSS2 knockout in mCCDcl1 cells compromised γ-ENaC cleavage and reduced baseline and aldosterone-stimulated I
SC, which could be rescued by chymotrypsin. A compensatory transcriptional upregulation of other proteases was not observed. Tmprss2
−/− mice kept on standard diet exhibited no apparent phenotype, but renal γ-ENaC cleavage was altered. In response to a low-salt diet, particularly with high potassium intake, Tmprss2
−/− mice increased plasma aldosterone significantly more than wild-type mice to achieve a similar reduction of renal sodium excretion. Importantly, the stimulatory effect of trypsin on renal tubular ENaC currents was much more pronounced in Tmprss2
−/− mice than that in wild-type mice. This indicated the presence of incompletely cleaved and less active channels at the cell surface of TMPRSS2-deficient tubular epithelial cells.

Conclusions

TMPRSS2 contributes to proteolytic ENaC activation in mouse kidney in vivo.
Uso corporativo da mídia social X e a previsão dos analistas
Authors: Giordani Mikaéli da Silva , Klann Roberto Carlos
Published: October 2024

Este trabalho objetivou investigar se o uso corporativo do X (antigo Twitter), para divulgação de informações financeiras, bem como curtidas, comentários e retweets relacionados a essas informações, podem melhorar a precisão da previsão dos analistas. A disseminação de informações no X sinaliza evidências adicionais para os interessados na organização, como os analistas. Foi operacionalizada regressão linear múltipla, com erros padrão robustos. A análise consistiu em 2.548 observações, no período de 2013 a 2019. Os resultados demonstram que o erro da previsão dos analistas é maximizado de acordo com as reações das publicações. As curtidas, comentários e retweets do trimestre anterior influenciam a precisão da previsão dos analistas no trimestre atual. Uma possível explicação para este resultado pode residir no fato de que as empresas publicam informações demasiadamente otimistas. Isso leva a uma reação otimista por parte dos usuários, consequentemente, induzindo os analistas ao erro. A análise adicional verificou a interação entre empresas com lucro no período e as variáveis do X. Os resultados da interação das variáveis com o lucro das empresas, indicaram que empresas lucrativas que tweetam demonstram ter um erro maior de previsão dos analistas, em comparação com empresas lucrativas que não tweetam. A pesquisa evidenciou que as mídias sociais podem ser um importante canal para disseminar informações financeiras pelas empresas. Ademais, a interação entre empresas e stakeholders, ou entre os próprios stakeholders no X, facilita a comunicação, circulação de informações e possibilita o feedback, aspectos que, em conjunto, influenciam os interessados na empresa, como os analistas.
Shear Capacity Prediction of Extremely-Loaded Box Culvert on Elastic Soil Using Artificial Neural Network
Authors: Tuskan Yesim , Yıldırım Uncu Dilay
Published: October 2024

A box culvert, buried at shallow depths beneath roadways, may experience deflections caused by the dynamic impact of traffic loading and the vertical pressure exerted by the soil fill. A computational model commonly employed used to various engineering issues, including those in geotechnical applications, is the beam-on-elastic-foundation model. In this context, the Moment Distribution Method (MDM) must be applied to account for the elastic foundation. To achieve this, the internal forces acting on the ends of both exterior and interior walls are transferred to the beam-like bottom slab of the culvert, which rests on an elastic soil bed. Subsequently, the secondary internal forces are determined by refining the structural parameters, taking into account the characteristics of the elastic soil bed. This study presents the development and application of an Artificial Neural Network (ANN) model to predict the shear capacity of box culverts on elastic soil under traffic loading conditions. The proposed model is trained and validated using a comprehensive database of beam on elastic foundation solutions. The input parameters include the geometrical and mechanical properties of the culvert and the soil, as well as the loading conditions. The results of the ANN model show R2 values of 0.9633 and 0.9581 for the training and testing sets, respectively, indicating the model's excellent accuracy. These findings suggest that the ANN model can reliably predict the shear capacity of culverts.
Fishery characteristics, population dynamics and the impacts of the invasive red swamp crayﬁsh (Procambarus clarkii) in the Nile River, Egypt
Authors: Mehanna Sahar Fahmy
Published: October 2024

Alien intrusive species are one of the extreme pressures on biodiversity for their rapid reproduction and adaptation to new environments. Crayfish Procambarus clarkii entered Egyptian freshwater ecosystems at the beginning of 1980's. After many years of negative effects of its introduction onto the Egyptian waters, it became one of the commercially important species. Population parameters of P. clarkii are estimated based on 1355 specimens collected from the Nile off El-Minya during 2019–2020. The isometric growth of the species under the study is confirmed according to the obtained b-value of length–weight relationship (LWR). Annual sex ratio (female : male) was in favour of females with an annual sex ratio 1.44 : 1. The values of growth coefficient (K) and the asymptotic total length (L∞) were assessed for males, females and combined sexes. Using mortality estimates, the exploitation ratio was 0.64 year–1. Investigation of relative yield per recruit (Y'/R) and analysis of relative biomass per recruit (B'/R) for P. clarkii in the Nile off El-Minya provides the highest (Y'/R) at Emax = 0.81 and the E0.5 (utilisation level that preserves the spawner stock biomass at 50% of the virgin spawning biomass) was 0.38. From fisheries management point of view, the current exploitation level should be decreased to that maintain the spawning stock biomass (from 0.64 to 0.38).
Las disposiciones educativas y laborales de egresados de secundaria. Un estudio de caso comparativo
Authors: Montoya Arias Diana Milena , Castellanos Obregón Juan Manuel
Published: October 2024

Este artículo busca identificar la influencia de las prácticas socializadoras familiares y escolares en las disposiciones laborales y educativas de los jóvenes egresados de un colegio público de un municipio pequeño. La investigación se realizó mediante un enfoque de estudio de caso comparativo, con entrevistas a profundidad a 24 jóvenes egresados de las cohortes graduadas en los años 2018 y 2019, sus padres y los docentes de la institución. Se comparan las condiciones causales de las socializaciones familiares y escolares de cada individuo con respecto a las trayectorias elegidas presentadas como resultado de “enrutamiento” usando técnicas analíticas como el análisis de clúster, el árbol de decisiones (chi cuadrado) y el análisis de correspondencias. La información se interpretó mediante las categorías de disposición, estrategia y sentido práctico en el sentido dado por Pierre Bourdieu (2000, 2008, 2011). En el análisis se identificó cómo la interacción entre las agencias familia y escuela forma propensiones y capacidades asociadas con una estrategia socializadora en la que incide en el tiempo, la consistencia y la radicalidad de sus socializaciones. El estudio da como resultado dos agrupamientos formados por quienes construyeron una relación con la academia como una oportunidad abierta de opciones; y quienes no lograron construir el interés ni tenían las condiciones para hacerlo, no accedieron a lo que hubiera disponible y en conclusión no se orientaron.
Perfiles de gravedad semántica y desempeño de estudiantes en el tema de pH en química
Authors: Sierra Vargas Johny , Ruiz Ortega Francisco Javier
Published: October 2024

La comunicación en el aula de ciencias naturales es crucial para el aprendizaje. Esta investigación analizó el código semántico "gravedad semántica" en clases de química de cuatro docentes de la ciudad de Manizales, Colombia. El objetivo de la investigación fue identificar cómo la gravedad semántica del docente se relaciona con la gravedad semántica de los estudiantes y sus desempeños en el tema de pH en química. En la investigación, que es de tipo descriptivacomprensiva, participaron docentes y estudiantes de cuatro instituciones educativas públicas. Se grabaron clases y se analizó el discurso para identificar momentos de gravedad semántica débil y fuerte, correspondientes al nivel de lejanía o cercanía al contexto y cotidianidad de los estudiantes. Se crearon perfiles de discurso para los docentes, y se correlacionaron con resultados estudiantiles en pruebas con pregunta abierta. Se encontró que un discurso docente dinámico facilita el aprendizaje de conceptos científicos. Además, se evidenció que los perfiles de gravedad semántica de los docentes influyen en el desempeño de los estudiantes. Por otra parte, se observó que algunos docentes lograron relacionar conceptos abstractos con situaciones cotidianas, lo que motivó a los estudiantes a comprender mejor los saberes propios del área de química, específicamente en el tema de pH.
Relationship between predation and maternal investment In Curly-Tailed Lizards
Authors: Consuegra Valentina , Flemming Adania , Claunch Natalie
Published: October 2024

The Northern Curly-tailed Lizard (Leiocephalus carinatus armouri) is invasive in Florida, where it has establishedmultiple populations. Like most animals, the species faces various environmental pressures, including predation,which influences reproductive strategies. To test the hypothesis that a correlation exists between predation rates onfemales and offspring-resource allocation, we examined specimens from 11 populations in Florida, assuming that thosewith broken tails had encountered a potential predator. To quantify offspring-resource allocation, we used the numberof ovarian follicles in each female and the ratio of number to average size of the largest follicles. We predicted that thosetraits would differ between lizards with and without broken tails. We did not detect the expected correlation, suggestingthat predation pressure did not affect the number or size of follicles. Consistent with other lizard species, we did findthat larger body size was correlated with a greater number of follicles.
Fresh Cell Isolation RNA Extraction v1
Authors: G Tansey Malu
Published: October 2024

This protocol describes the process of extracting RNA from freshly isolated immune cell populations using the Qiagen RNeasy Mini Kit.
West Nile Virus NS2B-NS3 protease fusion protein expression and purification: large scale 1 L cultures v1
Authors: fairhead michael
Published: October 2024

This method describes a protocol for the 1 L scale expression and purification of West Nile Virus NS2B-NS3 protease fusion protein in E. coli using autoinduction growth media. After enzyme detergent based cell lysis the fusion protein is purified using his tag chromatography, TEV protease tag cleavage, a reverse chromatography step and finally gel filtration. Final yield is 47 mg of West Nile Virus NS2B-NS3 protease fusion protein per litre of culture grown
BSA: The Bloch Sphere Approachas a Geometrical Design Tool for Building Cost-Effective Quantum Gates v1
Authors: Al-Bayaty Ali , Perkowski Marek
Published: October 2024

The Bloch sphere is a geometrical 3D sphere that visualizes the states of a qubit after a series of quantum gates are applied to it. For that, the Bloch sphere is mainly used as a geometrical visualization (and verification) tool. On the other hand, in this protocol, we introduce the Bloch sphere as a geometrical design tool for building cost-effective quantum gates based on their rotational quantum operations in the XY-plane, which is the 2D circular top-view of the Bloch sphere. Collectively, the Bloch sphere and its XY-plane are termed the Bloch sphere approach (BSA). With the BSA, various generic and cost-effective quantum gates and libraries are designed for IBM quantum computers, using the symmetrical and semi-symmetrical structures [1-5], Clifford+T gates, and IBM native gates ( , , , and ), as follows. Quantum libraries (GALA-n [3, 6] and CALA-n [4, 7]), which have become part of the IBM Qiskit ecosystem [8] n-bit Toffoli gate [2-4] n-bit Boolean gates (AND, NAND, OR, NOR, implication, and inhibition) [3, 4] n-bit controlled- ( ) and controlled- ( ) gates [3, 4] n-bit Fredkin gate [3, 4] n-bit Miller gate [3, 4] Boolean-Phase SWAP gate (p-SWAP) [4, 5] Because the quantum operations of all IBM native "basis" gates mainly rotate around the X-axis and Z-axis of the Bloch sphere, we propose the XY-plane of the Bloch sphere for the BSA. However, the BSA can also be utilized to build generic and cost-effective quantum gates for other quantum computers, e.g., Intel, Google, and Rigetti, using different projectional planes of the Bloch sphere, e.g., the XZ-plane and the YZ-plane, based on the supported native gates for such quantum computers. Therefore, in this protocol, we introduce the BSA as an open geometrical framework for prospective quantum computing research.
Zika Virus NS2B-NS3 protease fusion protein his10-tagged protein expression and purification: large scale 1 L cultures v1
Authors: fairhead michael
Published: October 2024

This protocol details the expression and purification of Zika Virus NS2B-NS3 protease fusion protein with a his10-tag for biophysical assay at a 1 L culture scale. Recombinant proteins are expressed in Escherichia coli using the autoinduction method and then purified in using a IMAC, desalt, tag cleavage, reverse IMAC and gel filtration work flow.
Biomarkers of renal parenchymal damage in young children with congenital hydronephrosis: literature review
Authors: Klimov Vladislav N. , Deryugina Lyudmila A. , Krasnova Elena I. , Popyhova Era B. , Kazymov Dzhalal F.
Published: October 2024

Enlargement of the renal cavity system in a fetus is revealed at prenatal ultrasound in 1–5% of cases and can be caused by congenital hydronephrosis due to pelvic-ureteral junction obstruction, which is one of the most common nosologies in the structure of obstructive uropathies in childhood. A high risk of renal function decrease at the preclinical stage of disease development dictates a need to create highly informative diagnostic programs and treatment algorithms aimed at preventing complications. In this review, we discuss results of studies on diagnostic and prognostic value of cytokine biomarkers which are of a great interest in congenital hydronephrosis in young children. They include: kidney injury molecule-1, vascular endothelial growth factor-A, monocyte chemoattractant protein-1, neutrophil gelatinase-associated lipocalin, interleukin-1. Literature search for this review was made in Web of Science, PubMed, Russian Science Citation Index, CyberLeninka, Scopus databases.
Genre et stéréotypage médiatique: du procès des médias aux processus psycho-sociaux de facilitation ou de réactance
Authors: Chabrol Claude
Published: October 2024

Résumé: Les discours sociaux publiquement produits dans les sociétés occidentales contemporaines mettent en oeuvre des opérations de stéréotypages qui évoquent des représentations bien connues et parfois opposées, conformes ou non aux catégorisations psycho-sociales profondes. Leurs fonctions premières ne semblent pas être de renforcer les catégorisations de genre les plus traditionnelles ou de promouvoir leurs avatars modernes opposés, mais bien plutôt de les rendre compatibles entre elles, au risque de les vider de leurs significations premières au profit d’un Imaginaire social qui les concilierait toutes à la façon dont les mythes tentaient eux aussi pour Levi Strauss d’opérer une conciliation et un dépassement de représentations sociales contraires dans les sociétés antérieures. Ces discours suscitent aussi des phénomènes importants de résistance dits de “reactance”. Des psychologues proposent aussi de renforcer la résistance à cette persuasion qu’ils ont d’ailleurs souvent étudiée de près.Mots-clés: genre; medias; stéréotypage; catégorisation; réactance.Abstract: Social discourses publicly produced in contemporary Western societies implement stereotyping operations that evoke well-known representations and sometimes opposite representations, according to deep psycho-social categorizations or not. Their main functions do not seem to be the ones that reinforce the most traditional gender categorizations or promote their opposite modern avatars, but rather the one that makes them compatible with one another, at the risk of emptying their first meanings in favor of a social imaginary that would conciliate them all in the same way that myths also tried to operate a reconciliation and an overcoming of social representations contrary to previous societies with Levi Strauss.Keywords: Gender; Media; Stereotypes; Categorization; Reactance.
Com que se parece a Retórica da Guerra Cultural no debate sobre imigração
Authors: Dos Santos Frederico Rios C.
Published: October 2024

O presente artigo tem o objetivo de verificar como se manifesta a Retórica da Guerra Cultural no debate sobre imigração em dois dos maiores jornais cotidianos franceses, em termos de distribuição, o Le Figaro e o Le Monde, o primeiro mais à direita e o segundo mais à esquerda no espectro político. No lapso temporal de 2012 a 2017, período entre duas eleições presidenciais com resultados substancialmente distintos, foram selecionados todos os artigos de opinião que contivessem a palavra-chave “imigração” no mecanismo de busca, o que resultou em um total de 433 artigos, 345 do Le Figaro, e 88 do Le Monde. Chegou-se ao resultado de uma série de constantes retóricas encontradas que seriam típicas desse tipo de embate cultural sobre imigração, o que pode ser testado em outros contextos em futuras pesquisas.
palatalização dos segmentos /t/ e /d/ adjacentes a ditongo em registros de fala mossoroense
Authors: Ananias Thayná Cristina , Cunha Carla Maria
Published: October 2024

Este artigo focaliza a análise do fenômeno da palatalização dos segmentos /t/ e /d/ em onset silábico contíguo a ditongo, em Mossoró–RN. As postulações de Clements e Hume (1996) sobre Geometria de Traços, de Selkirk (1982) sobre o Modelo Autossegmental de Sílaba formam a base teórica da pesquisa, que transversalmente traz também à discussão características socioculturais dos falantes (ARAGÃO, 2006, 2020; CARDOSO; MOTA; PAIM, 2012; WEINREICH; LABOV; HERZOG, 2006). A metodologia de coleta de dados envolve dois Questionários Fonético-Fonológicos Lúdicos, um Questionário Fonético-Fonológico e uma Narrativa Semidirigida aplicados a seis informantes. As variáveis extralinguísticas contempladas são sexo, idade e escolaridade. Após a pesquisa de campo, os dados foram registrados em transcrição fonética de oitiva e alguns deles passaram por análise acústica. A pesquisa objetiva verificar as motivações para a palatalização nessa comunidade, até então conhecida como não palatalizante. Os resultados indicam que a palatalização, em Mossoró, é linguisticamente significativa, principalmente no contexto de /t/ e /d/ em onset compartilhando sílaba átona final de palavra com ditongo iniciado por [i] ou [j]. Ademais, propõe-se a seguinte interpretação: a palatalização ocorre mediante espraiamento de nó Vocálico de /i/ e a africação mediante espraiamento desse nó e de [+contínuo], ramificado diretamente do nó Raiz. Por fim, a análise revela que há indícios de palatalização mais frequente por parte das mulheres e dos mais jovens – quando levado em consideração o âmbito extralinguístico.
Diferença na produção de Expressões Não-Manuais por usuários fluentes em Libras como primeira ou segunda língua
Authors: Hanada Letícia Kaori , Barbosa Plinio Almeida
Published: October 2024

As Expressões Não-Manuais (ENMs), em combinação com outros parâmetros como configuração, localização, movimento e orientação da palma da mão, são responsáveis pelos sinais que contemplam o léxico das línguas de sinais. Essas ENMs são movimentos do corpo e da expressão facial (BAKER-SHENK; COKELY, 1980) que possuem funções como diferenciação lexical, participação na construção sintática e contribuição para processos de intensificação (PAIVA et al., 2018). O atual trabalho tem como objetivo comparar o uso dessas ENMs entre um surdo fluente em Língua Brasileira de Sinais (Libras) como primeira língua (L1) e um ouvinte, fluente em Libras, como segunda língua (L2), a partir de enunciados assertivos afirmativos e negativos, interrogativas parciais, imperativas, incluindo sentenças com expressão de intensidade e movimento de topicalização. Utilizando o programa ELAN, foi possível transcrever esses enunciados considerando movimentos de sobrancelha, olhos, nariz, bochechas, boca, cabeça e tronco. A hipótese inicial é a de que o sinalizante fluente em Libras como L2 intensificaria com certa frequência e com movimentos mais amplos sua produção de ENMs, considerando esta uma questão da identidade com uma L2 e, também, a sua intensificação sendo necessária, por razões didáticas. A análise quantitativa avaliou durações e amplitudes médias dos sinais manuais e ENMs e a análise qualitativa averiguou a presença e marcação de cada ENM em relação à cada tipo de sentença. Ambas as análises apontam que o participante surdo produziu as ENMs responsáveis pela marcação dos diferentes tipos de enunciados de forma mais sistemática e ampliou mais a produção de ENMs do que o participante intérprete.
Metáforas e metonímias multimodais nos cartazes Soviéticos antialcoolismo
Authors: Pinelli Erica
Published: October 2024

Nas últimas décadas, houve uma proliferação de estudos relativos à metáfora e à metonímia, segundo a definição da Teoria da Metáfora Conceitual. Recentemente, uma crescente atenção tem vindo a ser dedicada às metáforas e metonímias multimodais e ao papel persuasivo que desempenham na publicidade comercial e social. Neste trabalho, analisamos os cartazes soviéticos antialcoolismo publicados durante a campanha de propaganda de Gorbačëv contra o consumo de bebidas alcoólicas (1985-1988). De modo particular, interessa-nos o papel central de um elemento pictórico, a garrafa, metonímia que se traduz como os conceitos de alcool ou alcoolismo. Nos cartazes anti-alcoolismo dos anos oitenta, a imagem metonímica da garrafa entra frequentemente em interação com outros processos metafóricos. Nosso estudo vai precisamente focalizar a interação entre as metonimias desencadeadas por meio do uso pictório e/ou verbal da garrafa e o processo de (des)personalização ocorrido nesses contextos específicos. Alguns cartazes apresentam a garrafa como personificação para transmitir a mensagem de que o alcool é prejudicial para a vida privada dos indivíduos, pois pode manifestar-se enquanto um substituto dos entes queridos e levar às ações que independem da vontade do sujeito. Outros cartazes mostram a pessoa alcoolizada representada enquanto uma garrafa, para demonstrar que o alcoolismo destrói características humanas. A interação entre a imagem metonímica da garrafa e os processos de (des)personificação desempenha um papel primordial nos cartazes antialcoolismo soviéticos e visa representar os efeitos negativos do abuso de álcool na vida privada e social dos indivíduos.
Pedagogia dos multiletramentos e letramento multimodal crítico
Authors: Regner Ana Paula , Boas Gislaine Vilas
Published: October 2024

Este artigo tem por objetivo analisar um texto multimodal, o tweet, à luz da Gramática do Design Visual (GDV) com vistas à proposição de movimentos didático-pedagógicos em uma perspectiva de (multi)letramentos críticos. O corpus de análise é constituído do texto “Ataque a tiros em escola do ES: Por que ataques em escolas têm se repetido no Brasil?”, publicado no Twitter do Jornal do Estado de São Paulo (Estadão), em novembro de 2022. Os procedimentos de análise compreendem: i) analisar e descrever, a partir da GDV, os significados representacionais, interativos e composicionais que constituem os recursos imagéticos do texto (Kress; van Leeuwen, 2020) e ii) apresentar proposta de exploração didática para ser desenvolvida nas aulas de língua portuguesa, com base na Pedagogia dos Multiletramentos (Kalantzis et al., 2020).
Modelo para la gestión del conocimiento e innovación en escenarios de educación virtual de la Universidad del Quindío
Authors: Ramírez Agudelo Angélica María , Giraldo Gaviria Alejandra , Parra Sánchez Alejandra María
Published: October 2024

El conocimiento es un tema de interés para aquellos que reconocen su impacto como activo intangible en las organizaciones, incluidas las instituciones de educación superior, las cuales han incorporado en su quehacer académico las TIC y el modelo e-learning para el desarrollo de sus procesos de formación y gestión. El presente trabajo propone el diseño de un modelo de gestión del conocimiento e innovación en escenarios de educación virtual para instituciones de educación superior, partiendo de los ejes misiónales como la extensión, docencia e investigación, en las que se evidencian estrategias (desde el hacer) y buenas prácticas (desde el ser), las cuales confluyen en cuatro elementos integrativos del modelo propuesto, que parten de la construcción de saberes y aprendizajes mediante la implementación de los procesos de socialización, combinación, exteriorización e interiorización propuestos por Nonaka y Takeuchi (1995). Estos procesos sin duda dinamizan la formación en escenarios de educación virtual, a distancia y presencial. Todos estos elementos, dinámicos desde su concepción, están constituidos por un capital humano, un capital estructural (tecnología), un capital relacional (cultura organizacional) y la motivación e innovación (sector educativo). Estos elementos son presentados a fin de consolidar la Gestión del Conocimiento e Innovación en escenarios de educación virtual, que día a día toma mayor auge en la Universidad del Quindío y la educación global.
How Effective are Active Labour Market Policies and Public Employment Services?: Lessons for Latin America and the Caribbean
Authors: Nunn Alex
Published: October 2024

Active Labour Market Policies/Programmes (ALMPs) include a range of interventions such as training, employment incentives, and subsidized employment, with Public Employment Services (PES) being a central element. PES typically provide intermediation services for jobseekers and employers, disseminate labor market information, and coordinate other ALMPs like training programs. ALMPs differ from passive labor market measures, which involve unemployment benefits or cash transfers. In many countries, PES administer these benefits and combine them with active measures, particularly where passive programs are more generous. In Latin America and the Caribbean (LAC), countries invest in ALMPs and PES, particularly focusing on youth training, but overall spending on active and passive measures remains below OECD averages. Empirical studies on ALMP effectiveness suggest that well-targeted and well-funded programs are more successful. PES services tend to show small but positive short-term impacts, while training programs often exhibit long-term benefits, such as increased employment formality in LAC. Other programs show variable results. The main question is not whether ALMPs work, but how they can be designed to address the needs of specific groups in different contexts. Evidence shows that aligning ALMPs with socio-economic conditions and including ongoing evaluation and adaptation are key to improving outcomes. Thus, a focus on program design, participant selection, and performance management is crucial to ensure the effectiveness of ALMPs across different labor market environments.
Revolucionando la tributación: la administración tributaria 3.0 ya está aquí
Authors: Calijuri Mónica
Published: October 2024

Los gobiernos a nivel global reconocen la necesidad de una recaudación de impuestos eficiente y transparente para fomentar la equidad entre los ciudadanos. La administración tributaria 3.0 se caracteriza por la utilización avanzada de datos y la automatización, lo que abarca la identidad digital, los puntos de contacto con los contribuyentes, la gestión de datos y estándares, la gestión de reglas tributarias, nuevas habilidades y marcos de gobernanza. Esta publicación profundiza en los elementos clave de la administración tributaria 3.0, y resalta el cambio desde la gestión manual de impuestos (administración tributaria 1.0) hacia un enfoque integral basado en datos, que hace hincapié en la transparencia y la cooperación. En el centro de esta transformación están los datos, los cuales facilitan prácticas innovadoras, como declaraciones de impuestos prellenadas, análisis de datos y modelado predictivo para combatir el fraude y mejorar la equidad en el sistema tributario. Aunque la digitalización promete una mayor eficiencia y participación de los contribuyentes, también requiere una gestión proactiva de la seguridad y privacidad de los datos para mantener la confianza pública.
Evaluación de la madurez de la gestión de activos públicos
Authors: García Mejía Mauricio , Aguirre De Mora Florencia , Brankevich Paula , Roseth Benjamin , Naranjo Bautista Sandra
Published: October 2024

Este documento presenta un marco para la Evaluación de la Madurez de la Gestión de Activos Públicos (EMGAP), que permite diagnosticar de manera precisa el grado de desarrollo de la gestión de activos públicos en los países. Los cimientos de una gestión eficiente de activos públicos incluyen unas políticas y marcos normativos coherentes, un liderazgo claramente definido, procedimientos operativos estándar formalizados y mejorados continuamente, estructuras organizativas apropiadas, financiación suficiente y estable, así como recursos tecnológicos adecuados para respaldar las actividades a realizar. A su vez, los sistemas deben nutrirse de información completa y actualizada, de manera que permitan contar con una caracterización completa de los bienes disponibles, detallar y controlar el riesgo de las decisiones tomadas sobre estos bienes y elaborar indicadores para medir resultados y asignar responsabilidades. Finalmente, se requiere contar con planes estratégicos que permitan adoptar una visión a largo plazo de la cartera de activos y de los activos individuales. Este diagnóstico, junto con el ideal de dónde la organización desea estar en el futuro, proporciona la base para diseñar un plan de mejora, rastrear el progreso y cumplir con el objetivo de modernizar la gestión de los activos públicos, para así maximizar el valor socioeconómico que estos generan.
Development and research testing of a low thrust on gaseous-propellant rocket engine chamber
Authors: Musteikis A. I. , Levikhin A. A. , Kolosenok S. V.
Published: October 2024

The article presents preliminary results of the development and research testing of a rocket engine chamber with a thrust of 100 N operating on fuel components: gaseous oxygen-gaseous hydrogen, designed to be used as the main engine of a small upper stage for launching a payload weighing up to 150 kg into target orbits. The main features of the engine chamber are its fabrication by selective laser melting from 12X18N10T steel powder and its regenerative cooling with gaseous hydrogen. Calculation and experiments confirmed the possibility of regenerative cooling of the chamber in the nominal mode. In addition, the current results of the development of a technique for optical recording of the process of structural material removal from the chamber during tests using different optical filters are presented. It is shown that there is a correlation between the brightness of the obtained frames and the hydrogen flow rate. It is also shown that the afterburning of the removed material particles, in contrast to high thrust engines, occurs mainly in the tail of the jet.
Stuck at the Bottom? Gender and Immigrants’ Entrapment in Low-skilled Work
Authors: Grönlund Anne , Nordlund Madelene
Published: October 2024

Immigrant women are widely portrayed as facing a serious labor market disadvantage, yet gender differences in immigrants’ occupational mobility have been little explored. The article studied gender gaps in immigrants’ occupational entrapment, defined as entering and remaining in low-skilled/low-prestige work rather than moving on to more skilled/prestigious work. The analyses were based on longitudinal register data and followed five immigrant cohorts arriving in Sweden in the early 2000s over a 12-year period. The results show that immigrant women were more likely than men to start out in a low-skilled/low-prestige job and less likely to experience occupational mobility. However, tertiary education strongly mitigated these gender gaps. At the same time, the differences between high- and low-educated individuals widened more among women than among men. Family-related factors and country-specific human capital did not explain these patterns, but the findings indicate that occupational gender segregation can be crucial to immigrants’ mobility prospects.
Trauma-informed practice as a component to improve student wellbeing
Authors: Julies Yolanda
Published: October 2024

Trauma-informed schools are deeply responsive to the trauma experiences of students and address their primary need for safety and connection as a foundation for emotional, social, and cognitive wellbeing. Trauma-informed schools have systems in place that provide trauma awareness, knowledge, and skills as part of their school culture, practices, and policies. This article draws on a literature base to explore what it means to be trauma-informed and shows ways that schools can play a key role in addressing the effects of traumatic stress on students.