bluuebunny/crossref_metadata_2025 huggingface dataset:
Compound Heterozygous Mutations of SACS in a Korean Cohort Study of Charcot-Marie-Tooth Disease Concurrent Cerebellar Ataxia and Spasticity
Authors: Pi Byung Kwon , Chung Yeon Hak , Kim Hyun Su , Nam Soo Hyun , Lee Ah Jin , Nam Da Eun , Park Hyung Jun , Kim Sang Beom , Chung Ki Wha , Choi Byung-Ok
Published: June 2024

Mutations in the SACS gene are associated with autosomal recessive spastic ataxia of Charlevoix-Saguenay disease (ARSACS) or complex clinical phenotypes of Charcot-Marie-Tooth disease (CMT). This study aimed to identify SACS mutations in a Korean CMT cohort with cerebellar ataxia and spasticity by whole exome sequencing (WES). As a result, eight pathogenic SACS mutations in four families were identified as the underlying causes of these complex phenotypes. The prevalence of CMT families with SACS mutations was determined to be 0.3%. All the patients showed sensory, motor, and gait disturbances with increased deep tendon reflexes. Lower limb magnetic resonance imaging (MRI) was performed in four patients and all had fatty replacements. Of note, they all had similar fatty infiltrations between the proximal and distal lower limb muscles, different from the neuromuscular imaging feature in most CMT patients without SACS mutations who had distal dominant fatty involvement. Therefore, these findings were considered a characteristic feature in CMT patients with SACS mutations. Although further studies with more cases are needed, our results highlight lower extremity MRI findings in CMT patients with SACS mutations and broaden the clinical spectrum. We suggest screening for SACS in recessive CMT patients with complex phenotypes of ataxia and spasticity.
The Nucleolar Protein C1orf131 Is a Novel Gene Involved in the Progression of Lung Adenocarcinoma Cells through the AKT Signalling Pathway
Authors: Wei Zhili , Zhao Yiming , Cai Jing , Xie Yajun
Published: June 2024

Lung adenocarcinoma (LUAD) is the most widespread cancer in the world, and its development is associated with complex biological mechanisms that are poorly understood. Here, we revealed a marked upregulation in the mRNA level of C1orf131 in LUAD samples compared to non-tumor tissue samples in The Cancer Genome Atlas (TCGA). Depletion of C1orf131 suppressed cell proliferation and growth, whereas it stimulated apoptosis in LUAD cells. Mechanistic investigations revealed that C1orf131 knockdown induced cell cycle dysregulation via the AKT and p53/p21 signalling pathways. Additionally, C1orf131 knockdown blocked cell migration through the modulation of epithelial–mesenchymal transition (EMT) in lung adenocarcinoma. Notably, we identified the C1orf131 protein nucleolar localization sequence, which included amino acid residues 137–142 (KKRKLT) and 240–245 (KKKRKG). Collectively, C1orf131 has potential as a novel therapeutic marker for patients in the future, as it plays a vital role in the progression of lung adenocarcinoma.
Peptides Derived from the SARS-CoV-2 S2-Protein Heptad-Repeat-2 Inhibit Pseudoviral Fusion at Micromolar Concentrations: The Role of Palmitic Acid Conjugation
Authors: Düzgüneş Nejat , Tao Zhihua , Zhang Yuxia , Krajewski Krzysztof
Published: June 2024

SARS-CoV-2 S-protein-mediated fusion is thought to involve the interaction of the membrane-distal or N-terminal heptad repeat (NHR) (“HR1”) of the cleaved S2 segment of the protein and the membrane-proximal or C-terminal heptad repeat (CHR) (“HR2”) regions of the protein. We examined the fusion inhibitory activity of a PEGylated HR2-derived peptide and its palmitoylated derivative using a pseudovirus infection assay. The latter peptide caused a 76% reduction in fusion activity at 10 µM. Our results suggest that small variations in peptide derivatization and differences in the membrane composition of pseudovirus preparations may affect the inhibitory potency of HR2-derived peptides. We suggest that future studies on the inhibition of infectivity of SARS-CoV-2 in both in vitro and in vivo systems consider the need for higher concentrations of peptide inhibitors.
CircRNA Regulation of T Cells in Cancer: Unraveling Potential Targets
Authors: Li Zelin , Yin Shuanshuan , Yang Kangping , Zhang Baojie , Wu Xuanhuang , Zhang Meng , Gao Dian
Published: June 2024

T lymphocytes play a critical role in antitumor immunity, but their exhaustion poses a significant challenge for immune evasion by malignant cells. Circular RNAs (circRNAs), characterized by their covalently closed looped structure, have emerged as pivotal regulators within the neoplastic landscape. Recent studies have highlighted their multifaceted roles in cellular processes, including gene expression modulation and protein function regulation, which are often disrupted in cancer. In this review, we systematically explore the intricate interplay between circRNAs and T cell modulation within the tumor microenvironment. By dissecting the regulatory mechanisms through which circRNAs impact T cell exhaustion, we aim to uncover pathways crucial for immune evasion and T cell dysfunction. These insights can inform innovative immunotherapeutic strategies targeting circRNA-mediated molecular pathways. Additionally, we discuss the translational potential of circRNAs as biomarkers for therapeutic response prediction and as intervention targets. Our comprehensive analysis aims to enhance the understanding of immune evasion dynamics in the tumor microenvironment by facilitating the development of precision immunotherapy.
Carboxymethyl β-Cyclodextrin Assistance for the 4-Nitrophenol Reduction Using Cobalt-Based Layered Double Hydroxides
Authors: Demeester Alexia , Douma Fatima , Cousin Renaud , Siffert Stéphane , Pourceau Gwladys , Wadouachi Anne , Ponchel Anne , Monflier Eric , Noël Sébastien
Published: June 2024

Cobalt-aluminum-layered double hydroxides containing carboxymethyl β-cyclodextrin (CMβCD) were synthesized by coprecipitation and evaluated as a cobalt source for the 4-nitrophenol reduction in an aqueous medium. Several physicochemical techniques (XRD, FTIR, TGA) indicated the intercalation of the anionic cyclodextrin without damages to the hydrotalcite-type structure. These lamellar cobalt-aluminum hybrid materials (CoAl_CMβCD) were evaluated in the 4-nitrophenol reduction and showed higher activities in comparison with the CMβCD-free standard material (CoAl_CO3). To rationalize these results, a set of experimental controls going from physical mixtures of CoAl_CO3 with different cyclodextrins to other cobalt-based materials were investigated, highlighting the beneficial effects of both the layered double hydroxide and CMβCD-based hybrid structures. CMβCD also showed a beneficial effect as an additive during the 4-nitrophenol reduction. CoAl_CO3, dispersed in a fresh CMβCD solution could be re-used for five successive cycles without the loss of activity.
Comparative Review on Cancer Pathology from Aberrant Histone Chaperone Activity
Authors: Lee Jiho , Bao Xiucong
Published: June 2024

Histone chaperones are integral to chromatin dynamics, facilitating the assembly and disassembly of nucleosomes, thereby playing a crucial role in regulating gene expression and maintaining genomic stability. Moreover, they prevent aberrant histone interactions prior to chromatin assembly. Disruption in histone chaperone function may result in genomic instability, which is implicated in pathogenesis. This review aims to elucidate the role of histone chaperones in cancer pathologies and explore their potential as therapeutic targets. Histone chaperones have been found to be dysregulated in various cancers, with alterations in expression levels, mutations, or aberrant interactions leading to tumorigenesis and cancer progression. In addition, this review intends to highlight the molecular mechanisms of interactions between histone chaperones and oncogenic factors, underscoring their roles in cancer cell survival and proliferation. The dysregulation of histone chaperones is significantly correlated with cancer development, establishing them as active contributors to cancer pathology and viable targets for therapeutic intervention. This review advocates for continued research into histone chaperone-targeted therapies, which hold potential for precision medicine in oncology. Future advancements in understanding chaperone functions and interactions are anticipated to lead to novel cancer treatments, enhancing patient care and outcomes.
Vitamin D and Sulforaphane Decrease Inflammatory Oxidative Stress and Restore the Markers of Epithelial Integrity in an In Vitro Model of Age-Related Macular Degeneration
Authors: Bergandi Loredana , Palladino Giulia , Meduri Alessandro , De Luca Laura , Silvagno Francesca
Published: June 2024

Age-related macular degeneration (AMD) is strictly linked to chronic oxidative stress, inflammation, loss of epithelial barrier integrity, and often with abnormal new blood vessel development. In this study, the retinal epithelial cell line ARPE-19 was treated with pro-inflammatory transforming growth factor-beta (TGF-β) to investigate the activity of vitamin D (VD) and sulforaphane (SF) in abating the consequences of oxidative stress and inflammation. The administration of VD and SF lowered reactive oxygen species (ROS) levels, and abated the related expression of the pro-inflammatory cytokines interleukin-6 and interleukin-8 induced by TGF-β. We evaluated mitochondrial respiration as a source of ROS production, and we discovered that the increased transcription of respiratory elements triggered by TGF-β was prevented by VD and SF. In this model of inflamed epithelium, the treatment with VD and SF also reduced the secretion of VEGF, a key angiogenic factor, and restored the markers of epithelial integrity. Remarkably, all the observed biological effects were potentiated by the co-stimulation with the two compounds and were not mediated by VD receptor expression but rather by the ERK 1/2 pathway. Altogether, the results of this study reveal the powerful synergistic anti-inflammatory activity of SF and VD and lay the foundation for future clinical assessment of their efficacy in AMD.
Understanding Local Government Cybersecurity Policy: A Concept Map and Framework
Authors: Hossain Sk Tahsin , Yigitcanlar Tan , Nguyen Kien , Xu Yue
Published: June 2024

Cybersecurity is a crucial concern for local governments as they serve as the primary interface between public and government services, managing sensitive data and critical infrastructure. While technical safeguards are integral to cybersecurity, the role of a well-structured policy is equally important as it provides structured guidance to translate technical requirements into actionable protocols. This study reviews local governments’ cybersecurity policies to provide a comprehensive assessment of how these policies align with the National Institute of Standards and Technology’s Cybersecurity Framework 2.0, which is a widely adopted and commonly used cybersecurity assessment framework. This review offers local governments a mirror to reflect on their cybersecurity stance, identifying potential vulnerabilities and areas needing urgent attention. This study further extends the development of a cybersecurity policy framework, which local governments can use as a strategic tool. It provides valuable information on crucial cybersecurity elements that local governments must incorporate into their policies to protect confidential data and critical infrastructure.
Dynamic Purkinje Meter as a Tool for Intraocular Lens Position Measurement
Authors: Palkovicova Eliska , Cendelin Jiri , Kudrna Petr
Published: June 2024

Due to the increasing demands of today’s society on visual quality and patient comfort, and due to the growing interest in the implantation of new and more complex intraocular lens (IOL) designs, determining the IOL position occupies an important position in current ophthalmological practice. The dynamic Purkinje meter combines the construction of static Purkinje meters, presented in recent years, with dynamic examination of the IOL position according to the optical axis of the IOL.
Linking Myocardial Infarction and Frailty Status at Old Age in Europe: Moderation Effects of Country and Gender
Authors: Sentandreu-Mañó Trinidad , Torres Zaira , Luján-Arribas Cecilia , Tomás José M. , González-Cervantes José Javier , Marques-Sule Elena
Published: June 2024

Background: Myocardial infarction (MI) is a serious condition affecting a considerable number of individuals, with important clinical consequences. Understanding the associated factors is crucial for effective management and prevention. This study aimed to (1) examine the association between MI and frailty in a sample of older European adults and (2) investigate the moderating effects of country and gender on this association. Methods: A cross-sectional survey of 22,356 Europeans aged 60 years and older was conducted. The data come from the sixth wave of the Survey of Health, Ageing and Retirement in Europe. Frailty, MI, gender, and country were studied. Results: Frailty is strongly associated with MI. Robust older adults are 13.31 times more likely not to have an MI. However, these odds drop to 5.09 if pre-frail and to 2.73 if frail. Gender, but not country, moderates this relationship. There is a strong association between MI and frailty in men, whereas for women, the association is not as strong. Conclusions: Frailty is highly associated with MI in European older adults. Country did not moderate the link between frailty and MI but gender does, with the relationship being notably stronger in men. The frailty–MI association remained significant even when controlling for a number of personal conditions and comorbidities.
Alternative Access for TAVR: Choosing the Right Pathway
Authors: Lutz Katherine , Asturias Karla M. , Garg Jasmine , Poudyal Abhushan , Lantz Gurion , Golwala Harsh , Doberne Julie , Politano Amani , Song Howard K. , Zahr Firas
Published: June 2024

Transcatheter aortic valve replacement (TAVR) has emerged as an alternative treatment option for patients with severe aortic stenosis regardless of surgical risk, particularly in those with a high and prohibitive risk. Since the advent of TAVR, transfemoral access has been the standard of care. However, given comorbidities and anatomical limitations, a proportion of patients are not good candidates for a transfemoral approach. Alternative access, including transapical, transaortic, transaxillary, transsubclavian, transcarotid, and transcaval, can be considered. Each alternative access has advantages and disadvantages, so the vascular route should be tailored to the patient’s characteristics. However, there is no standardized algorithm when choosing the optimal alternative vascular access. In this review, we analyzed the evolution and current evidence for the most common alternative access for TAVR and proposed an algorithm for choosing the optimal vascular access in this patient population.
A Case Study of a Rare Disease (Fructosemia) Diagnosed in a Patient with Abdominal Pain
Authors: Garbowski Leszek , Walasek Marzena , Firszt Rafał , Chilińska-Kopko Ewelina , Błażejewska-Gała Paulina , Popielnicki Daniel , Dzięcioł-Anikiej Zofia
Published: June 2024

Hereditary fructose intolerance is a rare genetic disorder that is inherited in an autosomal recessive manner, with mutations sometimes occurring spontaneously. Consuming fructose triggers biochemical abnormalities, disrupting liver processes like glycogenolysis and gluconeogenesis. Recent studies have revealed elevated intrahepatic fat levels in affected individuals. Symptoms include aversion to fructose-containing foods, hypoglycemia, liver and kidney dysfunction, and growth delays, with severe cases leading to liver enlargement, fatty liver disease, kidney failure, and life-threatening hypoglycemia. In this case study, we present a 20-month-old child with symptoms including difficulty passing stool, abdominal rigidity, abdominal pain with bloating and hypoglycemia. Initial clinical findings revealed elevated liver enzymes, a mildly enlarged hyperechoic liver, hypercholesterolemia, and borderline alpha-fetoprotein values. Diagnostic assessments identified hereditary fructose intolerance (HFI) with pathogenic variants in the ALDOB gene, along with a diagnosis of celiac disease. Genetic testing of the parents revealed carrier status for pathological aldolase B genes. This case underscores the importance of comprehensive clinical evaluation and genetic testing in pediatric patients with complex metabolic presentations.
An Integrated Approach to Cyber Risk Management with Cyber Threat Intelligence Framework to Secure Critical Infrastructure
Authors: El Amin Habib , Samhat Abed Ellatif , Chamoun Maroun , Oueidat Lina , Feghali Antoine
Published: June 2024

Emerging cyber threats’ sophistication, impact, and complexity rapidly evolve, confronting organizations with demanding challenges. This severe escalation requires a deeper understanding of adversary dynamics to develop enhanced defensive strategies and capabilities. Cyber threat actors’ advanced techniques necessitate a proactive approach to managing organizations’ risks and safeguarding cyberspace. Cyber risk management is one of the most efficient measures to anticipate cyber threats. However, it often relies on organizations’ contexts and overlooks adversaries, their motives, capabilities, and tactics. A new cyber risk management framework incorporating emergent information about the dynamic threat landscape is needed to overcome these limitations and bridge the knowledge gap between adversaries and security practitioners. Such information is the product of a cyber threat intelligence process that proactively delivers knowledge about cyber threats to inform decision-making and strengthen defenses. In this paper, we overview risk management and threat intelligence frameworks. Then, we highlight the necessity of integrating cyber threat intelligence and assessment in cyber risk management. After that, we propose a novel risk management framework with integrated threat intelligence on top of EBIOS Risk Manager. Finally, we apply the proposed framework in the scope of a national telecommunications organization.
Calcium Orthophosphate (CaPO4) Containing Composites for Biomedical Applications: Formulations, Properties, and Applications
Authors: Dorozhkin Sergey V.
Published: June 2024

The goal of this review is to present a wide range of hybrid formulations and composites containing calcium orthophosphates (abbreviated as CaPO4) that are suitable for use in biomedical applications and currently on the market. The bioactive, biocompatible, and osteoconductive properties of various CaPO4-based formulations make them valuable in the rapidly developing field of biomedical research, both in vitro and in vivo. Due to the brittleness of CaPO4, it is essential to combine the desired osteologic properties of ceramic CaPO4 with those of other compounds to create novel, multifunctional bone graft biomaterials. Consequently, this analysis offers a thorough overview of the hybrid formulations and CaPO4-based composites that are currently known. To do this, a comprehensive search of the literature on the subject was carried out in all significant databases to extract pertinent papers. There have been many formulations found with different material compositions, production methods, structural and bioactive features, and in vitro and in vivo properties. When these formulations contain additional biofunctional ingredients, such as drugs, proteins, enzymes, or antibacterial agents, they offer improved biomedical applications. Moreover, a lot of these formulations allow cell loading and promote the development of smart formulations based on CaPO4. This evaluation also discusses basic problems and scientific difficulties that call for more investigation and advancements. It also indicates perspectives for the future.
Conditioning Influence of Kaolinite Matrices on Flexural Strength of Raw Pressed Slurry Collected from Ceramic Tile Production Wastewater
Authors: Avram Simona Elena , Barbu Tudoran Lucian , Cuc Stanca , Borodi Gheorghe , Birle Bianca Violeta , Petean Ioan
Published: June 2024

Kaolinite is able to assure the high binding affinity of the filler particles of raw ceramic bodies. It acts as a matrix that strongly holds the other constituents’ particles in a compact structure. The slurry samples were characterized by XRD, mineralogical microscopy and SEM coupled with an EDX elemental analysis. The slurry collected from the ceramic tile production wastewaters had a significant amount of kaolinite (36%), mostly fine particles of 3 µm, less surrounding quartz (37%) and mullite (19%) particles of 5–100 µm in diameter and traces of lepidocrocite (8%). It is a dense paste with a relative moisture of 25%. The square bar of the slurry as received, pressed at a load of 350 N, had a flexural strength of 0.61 MPa. Increasing the moisture to 33% using regular water, followed by mechanical attrition at 2000 rpm for 5 min, resulted in a porous bar with a flexural strength of 0.09 MPa; by increasing the attrition speed to 6000 rpm, the microstructural homogenization was improved and the flexural strength was about 0.68 MPa. It seems that regular water does not assure an optimal moisture for the kaolinite matrix conditioning. Therefore, we used technological water at pH = 10, a moisture of 33% and attrition at 6000 rpm for 5 min, and the bar pressed at a load of 350 N had a flexural strength of 1.17 MPa. The results demonstrate that the bar moistened with technological water and an attrition regime assured a proper conditioning for the kaolinite matrix, achieving the optimal binding of the quartz and mullite particles under the pressing load. Bars with the optimal mixture were pressed at several loads, including 70, 140, 210 and 350 N, and the flexural strength was progressively increased from 0.56 MPa to 1.17 MPa. SEM fractography coupled with atomic force microscopy (AFM) revealed that the optimal moisture facilitated a proper kaolinite particle disposal regarding the quartz and mullite filler particles, and the progressive load assured the strong binding of the finest kaolinite platelets onto their surface.
An Assessment of Existing Sport-Related Concussion Guidelines in Ireland: The Need for a United Approach
Authors: Walshe Ayrton , Daly Ed , Ryan Lisa
Published: June 2024

In 2014, an Irish parliament white paper called for greater addressing of sport-related concussions (SRCs) in Ireland, requesting the adoption of the Concussion in Sport Group’s (CISGs) guidelines and greater consistency in SRC return to play (RTP) management. Ten years later, it is unclear how these requests have been addressed. Recently, the United Kingdom’s government centralised guidelines to one SRC document for all grassroots sports. This study aimed to investigate all publicly available SRC guidance in Irish sports and national governing bodies (NGBs) to determine if centralised guidelines are warranted. Sport Ireland and the Irish Federation of Sports were searched for all recognised NGBs and sports in Ireland. Websites were searched for any information pertaining to SRCs and data were extracted and collated in Microsoft Excel. In total, 15 of 83 sports and/or NGBs included SRC guidance, nine of which provided RTP protocols. Various iterations of the CISGs guidance and tools were implemented. Several sports with a documented SRC risk had no guidelines present. The findings indicate disjointed and outdated guidance across Irish sport. Additionally, there are sports with a documented concussion risk that have no SRC guidance available. This study provides support for centralised guidelines to be adopted in Irish grassroots sports.
A Multi-Shot Approach for Spatial Resolution Improvement of Multispectral Images from an MSFA Sensor
Authors: Yao Jean Yves Aristide , Ayikpa Kacoutchy Jean , Gouton Pierre , Kone Tiemoman
Published: June 2024

Multispectral imaging technology has advanced significantly in recent years, allowing single-sensor cameras with multispectral filter arrays to be used in new scene acquisition applications. Our camera, developed as part of the European CAVIAR project, uses an eight-band MSFA to produce mosaic images that can be decomposed into eight sparse images. These sparse images contain only pixels with similar spectral properties and null pixels. A demosaicing process is then applied to obtain fully defined images. However, this process faces several challenges in rendering fine details, abrupt transitions, and textured regions due to the large number of null pixels in the sparse images. Therefore, we propose a sparse image composition method to overcome these challenges by reducing the number of null pixels in the sparse images. To achieve this, we increase the number of snapshots by simultaneously introducing a spatial displacement of the sensor by one to three pixels on the horizontal and/or vertical axes. The set of snapshots acquired provides a multitude of mosaics representing the same scene with a redistribution of pixels. The sparse images from the different mosaics are added together to get new composite sparse images in which the number of null pixels is reduced. A bilinear demosaicing approach is applied to the composite sparse images to obtain fully defined images. Experimental results on images projected onto the response of our MSFA filter show that our composition method significantly improves image spatial resolution and minimizes reconstruction errors while preserving spectral fidelity.
Unveiling the Faunal Diversity in the Water Column Adjacent to Two Seamounts in the Deep Arabian Sea Using Environmental DNA Metabarcoding
Authors: Kaliyath Devika Raj , Abdulaziz Anas , Chekidhenkuzhiyil Jasmin , Koovapurath Useph Abdul Jaleel , Menon Nandini
Published: June 2024

The diversity of organisms inhabiting deep-sea ecosystems, such as seamounts, has hitherto remained under-studied. In this study, we report on the faunal diversity in the water column adjacent to the summit and periphery of two seamounts (SMS2 and SMS3) and an oxygen minimum zone site located away from the seamounts in the southeast Arabian Sea. Environmental DNA (eDNA) in the water column was metabarcoded using the Cytochrome Oxidase C subunit I (COI) gene marker and Oxford Nanopore sequencing technology. Hydrographic conditions revealed that the summits of the seamounts intersect with the core oxygen minimum zone between depths of 300 and 600 m. Comparisons of COI gene sequences with those in available databases, MIDORI and BOLD, indicated the existence of a diverse group of novel organisms in the study area. Crustaceans dominated (75–95%) in the summit and periphery of the SMS2 and the OMZ site, while Cnidaria (56–63%) and Chordata (55%), respectively, dominated the summit and periphery of SMS3. Overall, the current study highlights the broad diversity of organisms living in the water column around the seamounts and underscores the potential of eDNA for exploring them.
Hydrodynamic Characteristics Analysis and Mooring System Optimization of an Innovative Deep-Sea Aquaculture Platform
Authors: Zhang Lixin , Zhen Xingwei , Duan Qiuyang , Huang Yi , Chen Chao , Li Yangyang
Published: June 2024

As nearshore aquaculture spaces become saturated, the development of fisheries aquaculture for deep sea has become an inevitable trend. This paper proposes an innovative deep-sea aquaculture platform that incorporates a vessel-shaped main structure and a single-point mooring system. The potential flow theory and the Morison equation are utilized to calculate the hydrodynamic loads on the main structure and the netting and mooring systems, respectively. The deformation and force of the netting in current are simulated, and the accuracy of the analytical methods used is validated based on experimental results. The influences of the netting system on the hydrodynamic characteristics of the platform are analyzed. Optimization on the single-point mooring system is conducted under static and dynamic conditions, considering the influences of various mooring parameters, including mooring line length, buoyancy of buoys, and mass of sinkers. The patterns of changes in motion response, mooring line tension, and minimum touchdown length under different mooring parameters are calculated and analyzed. The results indicate that changes in mooring line length have minimal impact on the dynamic response of the platform and mooring system. The addition of appropriate buoys or sinkers can reduce the motion response of the platform and the tension in the mooring lines. Moreover, compared to adding buoys, incorporating sinkers more effectively enhances the overall safety and stability of the platform system.
Characteristics of Patients with Sarcoidosis with Emphasis on Acute vs. Chronic Forms—A Single Center Experience
Authors: Stjepanovic Mihailo , Maric Nikola , Belic Slobodan , Milin-Lazovic Jelena , Djurdjevic Natasa , Jankovic Jelena , Petrovic Masa , Peric Jovan , Tulic Ivan , Cvejic Jelena , Popevic Spasoje , Dimic Janjic Sanja , Mihailovic Vucinic Violeta
Published: June 2024

Sarcoidosis is a granulomatous disease of unknown etiology that can affect almost any organ. Although the acute form can have spontaneous regression, a certain number of patients can have a chronic form, which leads to an increase in mortality and a decrease in the quality of life. Considering that the risk factors are still unknown, we wanted to compare the characteristics of patients with acute and chronic forms of sarcoidosis in Serbia in order to determine significant differences between them with hopes of contributing to everyday clinical practice. A total of 2380 patients treated in our clinic were enrolled in this study. They were separated into the following two groups: 1126 patients with acute form and 1254 patients with chronic form. They were further compared by gender, smoking status, radiological status, exposition, biomarkers for sarcoidosis, organ involvement, and other comorbidities; the distribution of patients according to regions of Serbia was also noted. Statistical significance was found in radiological findings (p < 0.001), biomarkers (calcium in 24 h urine p < 0.001; chitotriosidase p = 0.001), and the affliction of organs (p < 0.001). The differences noted in this paper could help improve our understanding of this disease.
Local Perineal Capillary Perforator Flaps: A Minimally Invasive Technique for the Correction of Vulvar Stenosis
Authors: Caretto Anna Amelia , Garganese Giorgia , Fragomeni Simona Maria , Tagliaferri Luca , Fionda Bruno , Scambia Giovanni , Gentileschi Stefano
Published: June 2024

Background: Vulvar stenosis is a debilitating condition that compromises sexual function, urination, and the ability to undergo gynecological examinations. The purpose of this study is to describe the technique of capillary perforator perineal flaps (CPPF) for the correction of vulvar stenosis. Methods: We retrospectively examined patients with vulvar stenosis treated through surgical separation and reconstruction with CPPF. The procedure involved vulvar separation with the creation of a subsequent defect, repaired using a flap, harvested laterally to the labia majora including a capillary perforator and transferred through a subcutaneous tunnel to repair the vulvar defect. The functional outcome was evaluated with the Bradford scale, comparing the preoperative and postoperative scores using the Student’s t-test. Results: thirteen patients were included, three with stenosis following treatment for vulvar cancer and ten due to lichen sclerosus. In total, we analyzed 29 flaps, with an average size of 15.6 cm2. We always included just one perforator in the flap and no postoperative complications. Stenosis was resolved in all patients, with no recurrences one year after the surgery. The preoperative average severity of the stenosis was 2.3 + 0.6, reducing to 0.3 + 0.4 post-intervention, indicating a significant improvement (p < 0.01). Conclusions: CPPF has proven to be a quick and safe method for the reconstruction of vulvar stenosis.
Comparative Analysis of Frailty Scores for Predicting Adverse Outcomes in Hip Fracture Patients: Insights from the United States National Inpatient Sample
Authors: Forssten Maximilian Peter , Cao Yang , Mohammad Ismail Ahmad , Tennakoon Lakshika , Spain David A. , Mohseni Shahin
Published: June 2024

The aim of the current investigation was to compare the ability of several frailty scores to predict adverse outcomes in hip fracture patients. All adult patients (18 years or older) who suffered a hip fracture due to a fall and underwent surgical fixation were extracted from the 2019 National Inpatient Sample (NIS) Database. A combination of logistic regression and bootstrapping was used to compare the predictive ability of the Orthopedic Frailty Score (OFS), the Nottingham Hip Fracture Score (NHFS), the 11-factor modified Frailty Index (11-mFI) and 5-factor (5-mFI) modified Frailty Index, as well as the Johns Hopkins Frailty Indicator. A total of 227,850 patients were extracted from the NIS. In the prediction of in-hospital mortality and failure-to-rescue (FTR), the OFS surpassed all other frailty measures, approaching an acceptable predictive ability for mortality [AUC (95% CI): 0.69 (0.67–0.72)] and achieving an acceptable predictive ability for FTR [AUC (95% CI): 0.70 (0.67–0.72)]. The NHFS demonstrated the highest predictive ability for predicting any complication [AUC (95% CI): 0.62 (0.62–0.63)]. The 11-mFI exhibited the highest predictive ability for cardiovascular complications [AUC (95% CI): 0.66 (0.64–0.67)] and the NHFS achieved the highest predictive ability for delirium [AUC (95% CI): 0.69 (0.68–0.70)]. No score succeeded in effectively predicting venous thromboembolism or infections. In summary, the investigated frailty scores were most effective in predicting in-hospital mortality and failure-to-rescue; however, they struggled to predict complications.
The Use of Microfiltration for the Pretreatment of Backwash Water from Sand Filters
Authors: Wolska Małgorzata , Kabsch-Korbutowicz Małgorzata , Rosińska Agata , Solipiwko-Pieścik Anna , Urbańska-Kozłowska Halina
Published: June 2024

Tests of microfiltration efficiency used for the pretreatment of backwash water from sand filters were conducted at two water treatment plants treating surface water and infiltration water. Microfiltration efficiency was evaluated for three membrane modules: two with polymeric membranes and one with a ceramic membrane. This study showed that the contaminants that limit the reuse of backwash water from both plants by returning them to the water treatment line are mostly microorganisms, including pathogenic species (Clostridium perfringens). Additionally, in the case of backwash water from infiltration water treatment, iron and manganese compounds also had to be removed before its recirculation to the water treatment system. Unexpectedly, organic carbon concentrations in both types of backwash water were similar to those present in intake waters. Microfiltration provided for the removal of organic matter, ranging from 19.9% to 44.5% and from 7.2% to 53.9% for backwash water from the treatments of surface water and infiltration water, respectively. Furthermore, the efficiency of the iron removal from backwash water from infiltration water treatment was sufficient to ensure good intake water quality. On the other hand, manganese concentrations in the backwash water, from infiltration water treatment, pretreated using the microfiltration process exceeded the levels found in the intake water and were, therefore, an additional limiting factor for the reuse of the backwash water. In both types of backwash water, the number of microorganisms, including Clostridium perfringens (a pathogenic one), was a limiting parameter for backwash water reuse without pretreatment. The results of the present study showed the possibility for using microfiltration for the pretreatment of backwash water, regardless of its origin but not as the sole process. More complex technological systems are needed before recirculating backwash water into the water treatment system. The polyvinylidene fluoride (PVDF) membrane proved to be the most effective for DOC and microorganism removal from backwash water.
Three-Dimensional-Printed Composite Structures: The Effect of LSCF Slurry Solid Loading, Binder, and Direct-Write Process Parameters
Authors: Yang Man , Parupelli Santosh Kumar , Xu Zhigang , Desai Salil
Published: June 2024

In this research, a direct-write 3D-printing method was utilized for the fabrication of inter-digitized solid oxide fuel cells (SOFCs) using ceramic materials. The cathode electrode was fabricated using the LSCF (La0.6Sr0.2Fe0.8Co0.2O3-δ) slurry loading and the Polyvinyl butyral (PVB) binder. The rheological parameters of slurries with varying LSCF slurry loading and PVB binder concentration were evaluated to determine their effect on the cathode trace performance in terms of microstructure, size, and resistance. Additionally, the dimensional shrinkage of LSCF lines after sintering was investigated to realize their influence on cathode line width and height. Moreover, the effect of the direct-write process parameters such as pressure, distance between the nozzle and substrate, and speed on the cathode line dimensions and resistance was evaluated. LSCF slurry with 50% solid loading, 12% binder, and 0.2% dispersant concentration was determined to be the optimal value for the fabrication of SOFCs using the direct-write method. The direct-write process parameters, in addition to the binder and LSCF slurry concentration ratios, had a considerable impact on the microstructure of cathode lines. Based on ANOVA findings, pressure and distance had significant effects on the cathode electrode resistance. An increase in the distance between the nozzle and substrate, speed, or extrusion pressure of the direct writing process increased the resistance of the cathode lines. These findings add to the ongoing effort to refine SOFC fabrication techniques, opening the avenues for advanced performance and efficiency of SOFCs in energy applications.
Study of the Rolling Effect on MoS2–Carbon Fiber Density and Its Consequences for the Functionality of Li-Ion Batteries
Authors: Wu Tai-Yu , Li Xiao-Ru , Chen Bo-Chun , Wang Li-Wen , Wang Jia-Hao , Chu Sheng-Yuan , Chang Chia-Chin
Published: June 2024

In this study, an electrode slurry composed of molybdenum disulfide (MoS2) and vapor-grown carbon fiber (VGCF) prepared through a solid-phase synthesis method was blade-coated onto copper foil to form a thick film as the anode for lithium-ion batteries. In previously reported work, MoS2-based lithium-ion batteries have experienced gradual deformation, fracture, and pulverization of electrode materials during the charge and discharge cycling process. This leads to an unstable electrode structure and rapid decline in battery capacity. Furthermore, MoS2 nanosheets tend to aggregate over charge and discharge cycles, which diminishes the surface activity of the material and results in poor electrochemical performance. In this study, we altered the density of the MoS2–carbon fiber/Cu foil anode electrode by rolling. Three different densities of electrode sheets were obtained through varying rolling repetitions. Our study shows the best electrochemical performance was achieved at a material density of 2.2 g/cm3, maintaining a capacity of 427 mAh/g even after 80 cycles.
Electrochemical Polishing of Ti and Ti6Al4V Alloy in Non-Aqueous Solution of Sulfuric Acid
Authors: Kołkowska Agata , Michalska Joanna , Zieliński Rafał , Simka Wojciech
Published: June 2024

This paper reports the results of our study on electrochemical polishing of titanium and a Ti-based alloy using non-aqueous electrolyte. It was shown that electropolishing ensured the removal of surface defects, thereby providing surface smoothing and decreasing surface roughness. The research was conducted using samples made of titanium and Ti6Al4V alloy, as well as implant system elements: implant analog, multiunit, and healing screw. Electropolishing was carried out under a constant voltage (10–15 V) with a specified current density. The electrolyte used contained methanol and sulfuric acid. The modified surface was subjected to a thorough analysis regarding its surface morphology, chemical composition, and physicochemical properties. Scanning electron microscope images and profilometer tests of roughness confirmed significantly smoother surfaces after electropolishing. The surface profile analysis of processed samples also yielded satisfactory results, showing less imperfections than before modification. The EDX spectra showed that electropolishing does not have significant influence on the chemical composition of the samples.
Coupons versus Rebates: An Economic–Mathematical Comparative Analysis with Policy Implications
Authors: Lin Tin-Chun
Published: June 2024

It is very important to understand how promotions offered by sellers to consumers influence consumer and seller behaviors because it helps us study how consumers make their purchase decisions and how sellers determine their sale strategies. Unlike past studies which utilized the classical price discrimination dilemma and focused on sellers’ aspect to divide consumers into two types (higher/lower reservation prices), we focused on the consumers’ perspective and applied neoclassical consumer choice theory to develop a two-period mathematical utility-maximization model to study how different promotions influence consumer and seller behaviors. This mathematical study uncovered several key findings: (1) coupons offer consumers a greater discount compared to rebates with identical discount rates; (2) impatient consumers exhibit a preference for coupons over rebates; (3) coupons generate higher sales than rebates; and (4) sellers may adopt the coupon policy for low-ticket products and the rebate policy for high-ticket products.
Nodal Invulnerability Recovery Considering Power Generation Balance: A Bi-Objective Robust Optimization Framework
Authors: Zhang Xueyang , Huang Shengjun , Li Qingxia , Wang Rui , Zhang Tao , Guo Bo
Published: June 2024

Nodal invulnerability has broad application prospects because of its emphasis on the differences between buses. Due to their long-term exposure, transmission lines are inevitably susceptible to damage caused by physical attacks or extreme weather. Therefore, restoring nodal invulnerability through a remedial approach or the introduction of mobile generators (MGs) is pivotal for resisting subsequent damage after a system is attacked. However, the research devoted to this field is limited. In order to fill the gap, this study conducts research on the configuration of MGs considering power generation balance to recover nodal invulnerability. First, a defender–attacker–defender (DAD) model is established, corresponding to the bi-objective robust optimization problem. The upper-level model is formulated to obtain the optimal compromise configuration scheme, the uncertainties of the attacked lines are elucidated in the middle level, and the nodal N−k security criterion utilized for measuring nodal invulnerability cooperates in the lower level. Then, a modified column-and-constraint generation (C&CG) algorithm is developed to incorporate fuzzy mathematics into the solution framework. In addition, the nodal invulnerability settings are optimized under limited resources. Numerical experiments are executed on the IEEE 24-bus system to verify the effectiveness and rationality of the proposed method.
Enhancing Control Systems through Type-3 Fuzzy Logic Optimization
Authors: Ochoa Patricia , Peraza Cinthia , Melin Patricia , Castillo Oscar , Park Seungmin , Geem Zong Woo
Published: June 2024

The advancement of new tools in the field of control systems is a contemporary development. This work introduces the utilization of Type-3 fuzzy logic, a relatively recent concept that has been applied across various disciplines. In our case, a Type-3 fuzzy system is designed to enhance the optimization of parameters within the harmony search algorithm, specifically tailored for a control problem. Through a series of experiments, the efficacy of this novel Type-3 fuzzy logic tool is put to the test. Previous studies have primarily explored Type-1 and Type-2 fuzzy logic. To assess the performance of this new Type-3 fuzzy logic tool, a comparative analysis of results is conducted using statistical testing. The introduction of Type-3 fuzzy logic in the control domain represents a novel and innovative approach. This approach extends beyond the conventional Type-1 and Type-2 fuzzy logic, showcasing the dynamic evolution in the field. Results obtained through experimentation are analyzed, and statistical tests are employed to determine whether the Type-3 fuzzy logic tool yields superior outcomes compared to its predecessors. By doing so, this study contributes to the growing body of research that explores the potential benefits of Type-3 fuzzy logic and its application in control systems, offering new perspectives and opportunities for further advancements in the field. We have to mention that the utilization of Type-3 fuzzy logic in enhancing metaheuristics is a relatively new trend, and in this work, this research has extended this to the realm of harmony search. In addition, the application of the optimal design of the ball-and-beam fuzzy controllers has not been previously carried out with the Type-3 fuzzy harmony search algorithm, which is the novelty of this study.
Dynamics of Infectious Diseases Incorporating a Testing Compartment
Authors: Yang Chayu , Deng Bo
Published: June 2024

In this paper, we construct an infectious disease model with a testing compartment and analyze the existence and stability of its endemic states. We obtain the basic reproduction number, R0, and demonstrate the existence of one endemic equilibrium without testing and one endemic equilibrium with testing and prove their local and global stabilities based on the value of the basic reproduction number, R0. We then apply our model to the US COVID-19 pandemic and find that, for a large parameter set, including those relevant to the SARS-CoV-2 virus, our analytic and numerical results suggest that the trajectories will be trapped to the testing-free state when the testing number is small enough. This indicates that the pandemic may end with a testing-free endemic state through a novel and surprising mechanism called stochastic trapping.
A Multi-Party Privacy-Preserving Record Linkage Method Based on Secondary Encoding
Authors: Han Shumin , Wang Yizi , Shen Derong , Wang Chuang
Published: June 2024

With the advent of the big data era, data security and sharing have become the core elements of new-era data processing. Privacy-preserving record linkage (PPRL), as a method capable of accurately and securely matching and sharing the same entity across multiple data sources, is receiving increasing attention. Among the existing research methods, although PPRL methods based on Bloom Filter encoding excel in computational efficiency, they are susceptible to privacy attacks, and the security risks they face cannot be ignored. To balance the contradiction between security and computational efficiency, we propose a multi-party PPRL method based on secondary encoding. This method, based on Bloom Filter encoding, generates secondary encoding according to well-designed encoding rules and utilizes the proposed linking rules for secure matching. Owing to its excellent encoding and linking rules, this method successfully addresses the balance between security and computational efficiency. The experimental results clearly show that, in comparison to the original Bloom Filter encoding, this method has nearly equivalent computational efficiency and linkage quality. The proposed rules can effectively prevent the re-identification problem in Bloom Filter encoding (proven). Compared to existing privacy-preserving record linkage methods, this method shows higher security, making it more suitable for various practical application scenarios. The introduction of this method is of great significance for promoting the widespread application of privacy-preserving record linkage technology.