bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-scale isotopic heterogeneity reveals a complex magmatic evolution: An example from the wallundry suite granitoids of the lachlan fold belt, Australia
Authors: Iles Kieran A. , Hergt Janet M. , Woodhead Jon D.
Published: February 2023

Open-system magmatic processes are expected to impart various sorts of isotopic heterogeneity upon the igneous rocks they produce. The range of processes under the “open-system” umbrella (e.g., simple two-component mixing, magma mingling, assimilation with fractional crystallization) cannot usually be uniquely identified using data from a single isotope system. The use of bulk-rock, mineral separate and in situ techniques and multiple isotope systems allows the characterization of isotopic variability at different sampling scales, illuminating details of the petrogenesis of a magmatic system. This approach has been applied to granitoids of the Wallundry Suite in the Lachlan Fold Belt, Australia. The Wallundry Suite exhibits variations in mineral assemblage, mineral composition and trends in bulk-rock major and trace element compositions consistent with the involvement of liquid-crystal sorting processes such as fractional crystallization. In situ paired O-Hf isotope data from zircon in six samples show an array indicating the isotopic evolution of the melt phase. Similarly, bulk-rock Sr-Nd-Hf isotope arrays support open-system magma evolution. These data combined with the petrographic observations and major and trace element geochemical variations suggest some form of assimilation-fractional crystallization process in the petrogenesis of the Wallundry Suite. Added complexity is revealed by two observations: 1) the isotopic variations are only weakly coupled to the lithology and major element compositions of the samples; and 2) there are distinguishable differences between the Hf isotope compositions of bulk-rock samples and those of the magmatic zircons they host. To varying degrees the rocks consistently show negative ΔεHfbulk-zrc values (i.e., the bulk-rock compositions have less radiogenic Hf isotope values than their coexisting zircons). The preservation of distinctly low Nd and Hf isotope ratios in an Fe-Ti oxide mineral separate suggests that the bulk-rock vs. zircon discrepancy is caused by the presence of unmelted components derived from a contaminant of continental origin (i.e., a rock with low Sm/Nd and Lu/Hf and thus unradiogenic Nd and Hf). Evidently, a complex interplay of assimilation, crystallization and melt segregation is required to account for the data. This investigation demonstrates that such complexity can, nevertheless, be disentangled through comparison of complementary isotope data at multiple sampling scales.
Reflecting on 21 years of running full PBL programs
Authors: Oliveira José Manuel Nunes de
Published: February 2023

The technological programs offered at the Higher Education Polytechnic School of Águeda, one of the Departments of the University of Aveiro, have been organized, since 2001, around the Project Based Learning paradigm. This paper will briefly set the scene for the educational development and will then proceed to give an historical perspective on the curriculum developments that took place along the last 21 years, reflecting the experience grown on the field. Finally, the author will offer his personal perspective on the difficulties and benefits of the development and its implementation, as an engaged agent in the process. Preliminary results of a study aiming at understanding the impact of the program in its graduate’s careers will also be mentioned.
Probiotics intervention in preventing conversion of impaired glucose tolerance to diabetes: The PPDP follow-on study
Authors: Yan Qun , Hu Weiting , Tian Yan , Li Xu , Yu Yuan , Li Xing , Feng Bo
Published: February 2023

Objectives

The purpose of this study was to assess the incidence of type 2 diabetes mellitus (T2DM) after 6 years in patients with IGT who received early probiotic intervention in the Probiotics Prevention Diabetes Program (PPDP) trial.

Methods

77 patients with IGT in the PPDP trial were randomized to either probiotic or placebo. After the completion of the trial, 39 non-T2DM patients were invited to follow up glucose metabolism after the next 4 years. The incidence of T2DM in each group was assessed using Kaplan-Meier analysis. The 16S rDNA sequencing technology was used to analyze gut microbiota’s structural composition and abundance changes between the groups.

Results

The cumulative incidence of T2DM was 59.1% with probiotic treatment versus 54.5% with placebo within 6 years, there was no significant difference in the risk of developing T2DM between the two groups (P=0.674).

Conclusions

Supplemental probiotic therapy does not reduce the risk of IGT conversion to T2DM.

Clinical Trial Registration

https://www.chictr.org.cn/showproj.aspx?proj=5543, identifier ChiCTR-TRC-13004024.
Correlation of multiple lipid and lipoprotein ratios with nonalcoholic fatty liver disease in patients with newly diagnosed type 2 diabetic mellitus: A retrospective study
Authors: Li Ran , Kong Dehong , Ye Zhengqin , Zong Guannan , Hu Kerong , Xu Wei , Fang Ping , Zhang Liya , Zhou Yun , Zhang Keqin , Xue Ying
Published: February 2023

Background and objective

The diagnostic value of lipid and lipoprotein ratios for NAFLD in newly diagnosed T2DM remains unclear. This study aimed to investigate the relationships between lipid and lipoprotein ratios and the risk of NAFLD in subjects with newly diagnosed T2DM.

Methods

A total of 371 newly diagnosed T2DM patients with NAFLD and 360 newly diagnosed T2DM without NAFLD were enrolled in the study. Demographics variables, clinical history and serum biochemical indicators of the subjects were collected. Six lipid and lipoprotein ratios, including triglycerides to high-density lipoprotein-cholesterol (TG/HDL-C) ratio, cholesterol to HDL-C (TC/HDL-C) ratio, free fatty acid to HDL-C (FFA/HDL-C) ratio, uric acid to HDL-C (UA/HDL-C) ratio, low-density lipoprotein-cholesterol to HDL-C (LDL-C/HDL-C) ratio, apolipoprotein B to apolipoprotein A1 (APOB/A1) ratio, were calculated. We compared the differences in lipid and lipoprotein ratios between NAFLD group and non-NAFLD group, and further analyzed the correlation and diagnostic value of these ratios with the risk of NAFLD in the newly diagnosed T2DM patients.

Results

The proportion of NAFLD in patients with newly diagnosed T2DM increased progressively over the range Q1 to Q4 of six lipid ratios, including the TG/HDL-C ratio, TC/HDL-C ratio, FFA/HDL-C ratio, UA/HDL-C ratio, LDL-C/HDL-C ratio, and APOB/A1 ratio. After adjusting for multiple confounders, TG/HDL-C, TC/HDL-C, UA/HDL-C, LDL-C/HDL-C and APOB/A1 were all strongly correlated with the risk of NAFLD in patients with newly diagnosed T2DM. In patients with newly-onset T2DM, the TG/HDL-C ratio was the most powerful indicator for the diagnosis of NAFLD among all six indicators, with an area under the curve (AUC) of 0.732 (95% CI 0.696–0.769). In addition, TG/HDL-C ratio>1.405, with a sensitivity of 73.8% and specificity of 60.1%, had a good diagnostic ability for NAFLD in patients with newly diagnosed T2DM.

Conclusions

The TG/HDL-C ratio may be an effective marker to help identify the risk of NAFLD in patients with newly diagnosed T2DM.
Impact of 6-month triptorelin formulation on predicted adult height and basal gonadotropin levels in patients with central precocious puberty
Authors: Yoo Eunjoo , Kim Sinae , Jung Hye Lim , Shim Jung Yeon , Shim Jae Won , Kim Deok Soo , Kwak Ji Hee , Kim Eun Sil , Yang Aram
Published: February 2023

Background

Triptorelin, a long-acting gonadotropin-releasing hormone (GnRH) agonist, is available in 1-, 3-, and 6-month formulations to treat central precocious puberty (CPP). The triptorelin pamoate 22.5-mg 6-month formulation recently approved for CPP offers greater convenience to children by reducing the injection frequency. However, worldwide research on using the 6-month formulation to treat CPP is scarce. This study aimed to determine the impact of the 6-month formulation on predicted adult height (PAH), changes in gonadotropin levels, and related variables.

Methods

We included 42 patients (33 girls and nine boys) with idiopathic CPP treated with a 6-month triptorelin (6-mo TP) formulation for over 12 months. Auxological parameters, including chronological age, bone age, height (cm and standard deviation score [SDS]), weight (kg and SDS), target height (TH), and Tanner stage, were evaluated at baseline, and after 6, 12, and 18 months of treatment. Hormonal parameters, including serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol for girls or testosterone for boys, were analyzed concurrently.

Results

The mean age at treatment initiation was 8.6 ± 0.83 (8.3 ± 0.62 for girls, 9.6 ± 0.68 for boys). The peak LH level following intravenous GnRH stimulation at diagnosis was 15.47 ± 9.94 IU/L. No progression of the modified Tanner stage was observed during treatment. Compared to baseline, LH, FSH, estradiol, and testosterone were significantly reduced. In particular, the basal LH levels were well suppressed to less than l.0 IU/L, and the LH/FSH ratio was less than 0.66. The bone age/chronological age ratio remained stable with a decreasing trend (1.15 at the start of treatment, 1.13 at 12 months, 1.11 at 18 months). PAH SDS increased during treatment (0.77 ± 0.79 at baseline, 0.87 ± 0.84 at the start of treatment, 1.01 ± 0.93 at six months, and 0.91 ± 0.79 at 12 months). No adverse effects were observed during treatment.

Conclusion

The 6-mo TP suppressed the pituitary-gonadal axis stably and improved the PAH during treatment. Considering its convenience and effectiveness, a significant shift to long-acting formulations can be expected.
Quantitative study of 3T MRI qDixon-WIP applied in pancreatic fat infiltration in patients with type 2 diabetes mellitus
Authors: Yi Jixing , Xu Fengming , Li Tao , Liang Bumin , Li Shu , Feng Qing , Long Liling
Published: February 2023

Objective

To investigate the application value of 3T MRI qDixon-WIP technique in the quantitative measurement of pancreatic fat content in patients with type 2 diabetes mellitus (T2DM).

Methods

The 3T MRI qDixon-WIP sequence was used to scan the livers and the pancreas of 47 T2DM patients (experimental group) and 48 healthy volunteers (control group). Pancreatic fat fraction (PFF), hepatic fat fraction (HFF), Body mass index (BMI) ratio of pancreatic volume to body surface area (PVI) were measured. Total cholesterol (TC), subcutaneous fat area (SA), triglyceride (TG), abdominal visceral fat area (VA), high density lipoprotein (HDL-c), fasting blood glucose (FPC) and low-density lipoprotein (LDL-c) were collected. The relationship between the experimental group and the control group and between PFF and other indicators was compared. The differences of PFF between the control group and different disease course subgroups were also explored.

Results

There was no significant difference in BMI between the experimental group and the control group (P=0.231). PVI, SA, VA, PFF and HFF had statistical differences (P<0.05). In the experimental group, PFF was highly positively correlated with HFF (r=0.964, P<0.001), it was moderately positively correlated with TG and abdominal fat area (r=0.676, 0.591, P<0.001), and it was weakly positively correlated with subcutaneous fat area (r=0.321, P=0.033). And it had no correlation with FPC, PVI, HDL-c, TC and LDL-c (P>0.05). There were statistical differences in PFF between the control group and the patients with different course of T2DM (P<0.05). There was no significant difference in PFF between T2DM patients with a disease course ≤1 year and those with a disease course <5 years (P>0.05). There were significant differences in PFF between the groups with a disease course of 1-5 years and those with a disease course of more than 5 years (P<0.001).

Conclusion

PVI of T2DM patients is lower than normal, but SA, VA, PFF, HFF are higher than normal. The degree of pancreatic fat accumulation in T2DM patients with long disease course was higher than that in patients with short disease course. The qDixon-WIP sequence can provide an important reference for clinical quantitative evaluation of fat content in T2DM patients.
Research on arc fault detection using ResNet and gamma transform regularization
Authors: Shuai Zhang , Qu Na , Zheng Tianfang , Hu Congqiang , Lu Senxiang
Published: February 2023

Series arc fault is the main cause of electrical fire in low-voltage distribution system. A fast and accurate detection system can reduce the risk of fire effectively. In this paper, series arc experiment is carried out for different kinds of electrical load. The time-domain current is analyzed by Morlet wavelet. Then, the multiscale wavelet coefficients are expressed as the coefficient matrix. In order to meet the data dimension requirements of neural networks, a color domain transformation method is used to transform the feature matrix into an image. A regularization method based on gamma transform is proposed for small sample data sets. The results showed that the proposed regularization method improved the validation set accuracy of ResNet50 from 66.67% to 96.53%. The overfitting problem of neural network was solved. In addition, this method fused fault features of 64 different scales, and provided a valuable manually labeled arc fault dataset. Compared with the threshold detection method, this method was more objective. The use of image features increased intuitiveness and generality. Compared with other typical lightweight networks, this method had the best detection performance.
Evaluation of the effectiveness of some local plant extracts in improving the quality of unsafe water consumed in developing countries
Authors: Konkobo Frédéric Anderson , Savadogo Paul Windinpsidi , Diao Mamounata , Dakuyo Roger , Dicko Mamoudou Hama
Published: February 2023

This study highlights the possibility of using plant extracts as biocoagulants to replace aluminum sulfate in the process of raw water potabilization. For this purpose, nine plants were identified by an investigation and their effectiveness was evaluated in the laboratory by coagulation/flocculation on surface waters. Out of the nine plants identified, five extracts showed a very low coagulant activity in the reduction of water samples turbidity (maximum abatement of 1.03%): These were the seeds of Acacia nilotica, Adansonia digitata, Balanites aegyptiaca, Tamarindus indica and leaves of Capparis corymbosa. Two extracts showed an average activity, namely Aloe vera sap (20.7%) and Opuntia ficus indica sap (32.25%). Two other extracts which are Moringa oleifera seeds and Boscia senegelensis seeds, showed a very good activity (84.83% and 82.97%, respectively after 1 h of decantation). By fixing the optimal concentration of 1 g/L for the treatment with Moringa oleifera seeds, a water of 4.6 NTU was obtained after 2 h of decantation, which was about 98% of abatement. The treatment with Boscia senegelensis seeds also allowed us to obtain for 2.5 g/L a water of 4.9 NTU after 2 h of decantation. The combined action of Moringa and Boscia biocoagulants, and cactus and Aloe mucilages reduced the decanting time to 15 min. These two treatments induced a slight increase of the minerals initially present in the water and a reduction of almost 99% of the pathogenic microorganisms. Thus, Boscia senegelensis and Moringa oleifera seeds appear as very effective biocoagulants compared to aluminum sulfate, hence they constitute an alternative to the lack of access to drinking water especially for developing countries.
Decision-making criteria for pesticide spraying considering the bees’ presence on crops to reduce their exposure risk
Authors: Decourtye Axel , Rollin Orianne , Requier Fabrice , Allier Fabrice , Rüger Charlotte , Vidau Cyril , Henry Mickaël
Published: February 2023

The risk of poisoning bees by sprayed pesticides depends on the attractiveness of plants and environmental and climatic factors. Thus, to protect bees from pesticide intoxication, an usual exemption to pesticide regulations allows for spraying on blooming flowers with insecticides or acaricides when no bees are foraging on crops. Nevertheless, decision-making criteria for farmers to assess the absence of bees on their crops remain under debate. To fill this gap, we present here a review of the literature and an analysis of weather conditions and environmental factors that affect the presence of bees on flowering crops that may be treated with pesticides, with the objective of proposing to farmers a series of decision-making criteria on how and when to treat. We conclude that the criteria commonly considered, such as ambient temperature, crop attractiveness, or distance from field edges, cannot guarantee the absence of forager exposure during pesticide sprays. Nocturnal sprays of pesticides on crops would be the most effective action to help farmers avoid unintentional acute poisoning of bees.
How voles adapt to subterranean lifestyle: Insights from RNA-seq
Authors: Bondareva Olga , Petrova Tatyana , Bodrov Semen , Gavrilo Maria , Smorkatcheva Antonina , Abramson Natalia
Published: February 2023

Life under the earth surface is highly challenging and associated with a number of morphological, physiological and behavioral modifications. Subterranean niche protects animals from predators, fluctuations in environmental parameters, but is characterized by high levels of carbon dioxide and low levels of oxygen and implies high energy requirements associated with burrowing. Moreover, it lacks most of the sensory inputs available above ground. The current study describes results from RNA-seq analysis of four subterranean voles from subfamily Arvicolinae:Prometheomys schaposchnikowi,Ellobius lutescens,Terricola subterraneus, andLasiopodomys mandarinus. Original RNA-seq data were obtained for eight species, for nine species, SRA data were downloaded from the NCBI SRA database. Additionally assembled transcriptomes of Mynomes ochrogaster and Cricetulus griseus were included in the analysis. We searched for the selection signatures and parallel amino acid substitutions in a total of 19 species. Even within this limited data set, we found significant changes ofdN/dSratio by free-ratio model analysis for subterranean Arvicolinae. Parallel substitutions were detected in genesRAD23BandPYCR2. These genes are associated with DNA repair processes and response to oxidative stress. Similar substitutions were discovered in theRAD23genes for highly specialized subterraneanHeterocephalus glaberandFukomys damarensis. The most pronounced signatures of adaptive evolution related to subterranean niche within species of Arvicolinae subfamily were detected forEllobius lutescens. Our results suggest that genomic adaptations can occur very quickly so far as the amount of selection signatures was found to be compliant with the degree of specialization to the subterranean niche and independent from the evolutionary age of the taxon. We found that the number of genomic signatures of selection does not depend on the age of the taxon, but is positively correlated with the degree of specialization to the subterranean niche.
Cotton plants as ideal models for teaching and research on inducible direct plant defenses
Authors: Mamin Marine , Vallat Armelle , Turlings Ted C. J.
Published: February 2023

Cotton (Gossypium hirsutum) stores defensive compounds in glands covering its leaves and other tissues. The density and the chemical filling of these glands increase systematically in developing leaves in response to herbivory on older leaves. Cotton seedlings are known to respond more strongly to actual caterpillar herbivory than to mere physical damage. It is not clear whether this amplified response is linked to insect-derived elicitors or difference in damage properties. To investigate this, we assessed the effect of repeated artificial damage without and with application of regurgitant from Spodoptera exigua caterpillars. Repeated mechanical damage led to a systemic increase of gland density, gland size, and content of defensive terpenes, with no detectable additional elicitation upon regurgitant treatment. Dual choice feeding assays further showed that defense induction triggered by just physical damage made newly developing leaves far less palatable to S. exigua larvae as compared to leaves from undamaged seedlings, whereas they did not distinguish between leaves from damaged plants treated with or without regurgitant. Our study confirms that the systemic induction of cotton glands is an unspecific response to physical damage, although cotton is known to respond to caterpillar-associated elicitors for other defensive traits. Cotton glands induction can be readily visualized under modest magnification, making the experiments described in this study highly suited to teach chemical ecology and aspects of plant defense theory in practical classes.
Signaling pathway of targeting the pancreas in the treatment of diabetes under the precision medicine big data evaluation system
Authors: Song Ge , Zhang Yiqian , Jiang Yihua , Zhang Huan , Gu Wen , Xu Xiu , Yao Jing , Chen Zhengfang
Published: February 2023

Diabetes is a chronic noncommunicable disease, which is related to lifestyle, environmental and other factors. The main disease of diabetes is the pancreas. Inflammation, oxidative stress and other factors can interfere with the conduction of various cell signaling pathways, thus inducing pancreatic tissue lesions and diabetes. Precision medicine covers epidemiology, preventive medicine, rehabilitation medicine and clinical medicine. On the basis of precision medicine big data analysis, this paper takes pancreas as the target to analyze the signal pathway of diabetes treatment. This paper analyzes from the five aspects of the age structure of diabetes, the blood sugar control standard of type 2 elderly diabetes mellitus, the changes in the number of diabetic patients, the ratio of patients using pancreatic species and the changes in blood sugar using the pancreas. The results of the study showed that targeted pancreatic therapy for diabetes reduced the diabetic blood glucose rate by approximately 6.94%.
Precision immunointerception of EGFR-driven tumorigenesis for lung cancer prevention
Authors: Pan Jing , Xiong Donghai , Zhang Qi , Palen Katie , Shoemaker Robert H. , Johnson Bryon , Sei Shizuko , Wang Yian , You Ming
Published: February 2023

Epidermal growth factor receptor (EGFR) mutations occur in about 50% of lung adenocarcinomas in Asia and about 15% in the US. EGFR mutation-specific inhibitors have been developed and made significant contributions to controlling EGFR mutated non-small cell lung cancer. However, resistance frequently develops within 1 to 2 years due to acquired mutations. No effective approaches that target mutant EGFR have been developed to treat relapse following tyrosine kinase inhibitor (TKI) treatment. Vaccination against mutant EGFR is one area of active exploration. In this study, we identified immunogenic epitopes for the common EGFR mutations in humans and formulated a multi-peptide vaccine (Emut Vax) targeting the EGFR L858R, T790M, and Del19 mutations. The efficacy of the Emut Vax was evaluated in both syngeneic and genetic engineered EGFR mutation-driven murine lung tumor models with prophylactic settings, where the vaccinations were given before the onset of the tumor induction. The multi-peptide Emut Vax effectively prevented the onset of EGFR mutation-driven lung tumorigenesis in both syngeneic and genetically engineered mouse models (GEMMs). Flow cytometry and single-cell RNA sequencing were conducted to investigate the impact of Emut Vax on immune modulation. Emut Vax significantly enhanced Th1 responses in the tumor microenvironment and decreased suppressive Tregs to enhance anti-tumor efficacy. Our results show that multi-peptide Emut Vax is effective in preventing common EGFR mutation-driven lung tumorigenesis, and the vaccine elicits broad immune responses that are not limited to anti-tumor Th1 response.
Neoadjuvant immune checkpoint inhibition in the management of glioblastoma: Exploring a new frontier
Authors: Frederico Stephen C. , Darling Corbin , Bielanin John P. , Dubinsky Alexandra C. , Zhang Xiaoran , Hadjipanayis Constantinos G. , Kohanbash Gary
Published: February 2023

Brain tumors are one of the leading causes of cancer related death in both the adult and pediatric patient population. Gliomas represent a cohort of brain tumors derived from glial cell lineages which include astrocytomas, oligodendrogliomas and glioblastomas (GBMs). These tumors are known to grow aggressively and have a high lethality with GBM being the most aggressive tumor in this group. Currently, few treatment options exist for GBM outside of surgical resection, radiation therapy and chemotherapy. While these measures have been shown to marginally improve patient survival, patients, especially those diagnosed with GBM, often experience a recurrence of their disease. Following disease recurrence, treatment options become more limited as additional surgical resections can pose life threatening risk to the patient, patients may be ineligible for additional radiation, and the recurrent tumor may be resistant to chemotherapy. Immune checkpoint inhibitors (ICIs) have revolutionized the field of cancer immunotherapy as many patients with cancers residing outside the central nervous system (CNS) have experienced a survival benefit from this treatment modality. It has often been observed that this survival benefit is increased following neoadjuvant administration of immune checkpoint inhibitors as tumor antigen is still present in the patient which enables a more robust anti-tumor immune response. Interestingly, results for ICI-based studies for patients with GBM have been largely disappointing which is a stark contrast from the success this treatment modality has had in non-central nervous system cancers. In this review, we will discuss the various benefits of neoadjuvant immune checkpoint inhibition such as how this approach reduces tumor burden and allows for a greater induction of an anti-tumor immune response. Additionally, we will discuss several non-CNS cancers where neoadjuvant immune checkpoint inhibition has been successful and discuss why we believe this approach may provide a survival benefit for GBM patients. We hope this manuscript will foster future studies aimed at exploring whether this approach may be beneficial for patients diagnosed with GBM.
Extracorporeal photopheresis as an immunomodulatory treatment modality for chronic GvHD and the importance of emerging biomarkers
Authors: Bojanic Ines , Worel Nina , Pacini Carolina P. , Stary Georg , Piekarska Agnieszka , Flinn Aisling M. , Schell Kimberly J. , Gennery Andrew R. , Knobler Robert , Lacerda João F. , Greinix Hildegard T. , Pulanic Drazen , Crossland Rachel E.
Published: February 2023

Haematopoietic stem cell transplantation (HSCT) is the treatment of choice for malignant haematological diseases. Despite continuous improvements in pre- and post-transplantation procedures, the applicability of allo-HSCT is limited by life-threatening complications such as graft-versus-host disease (GvHD), engraftment failure, and opportunistic infections. Extracorporeal photopheresis (ECP) is used to treat steroid resistant GvHD with significant success. However, the molecular mechanisms driving its immunomodulatory action, whilst preserving immune function, require further understanding. As ECP is safe to administer with few significant adverse effects, it has the potential for earlier use in the post-HSCT treatment of GvHD. Thus, further understanding the immunomodulatory mechanisms of ECP action may justify more timely use in clinical practice, as well as identify biomarkers for using ECP as first line or pre-emptive GvHD therapy. This review aims to discuss technical aspects and response to ECP, review ECP as an immunomodulatory treatment modality for chronic GvHD including the effect on regulatory T cells and circulating vs. tissue-resident immune cells and consider the importance of emerging biomarkers for ECP response.
Thermoneutral housing shapes hepatic inflammation and damage in mouse models of non-alcoholic fatty liver disease
Authors: Oates Jarren R. , Sawada Keisuke , Giles Daniel A. , Alarcon Pablo C. , Damen Michelle S.M.A. , Szabo Sara , Stankiewicz Traci E. , Moreno-Fernandez Maria E. , Divanovic Senad
Published: February 2023

Introduction

Inflammation is a common unifying factor in experimental models of non-alcoholic fatty liver disease (NAFLD) progression. Recent evidence suggests that housing temperature-driven alterations in hepatic inflammation correlate with exacerbated hepatic steatosis, development of hepatic fibrosis, and hepatocellular damage in a model of high fat diet-driven NAFLD. However, the congruency of these findings across other, frequently employed, experimental mouse models of NAFLD has not been studied.

Methods

Here, we examine the impact of housing temperature on steatosis, hepatocellular damage, hepatic inflammation, and fibrosis in NASH diet, methionine and choline deficient diet, and western diet + carbon tetrachloride experimental models of NAFLD in C57BL/6 mice.

Results

We show that differences relevant to NAFLD pathology uncovered by thermoneutral housing include: (i) augmented NASH diet-driven hepatic immune cell accrual, exacerbated serum alanine transaminase levels and increased liver tissue damage as determined by NAFLD activity score; (ii) augmented methionine choline deficient diet-driven hepatic immune cell accrual and increased liver tissue damage as indicated by amplified hepatocellular ballooning, lobular inflammation, fibrosis and overall NAFLD activity score; and (iii) dampened western diet + carbon tetrachloride driven hepatic immune cell accrual and serum alanine aminotransferase levels but similar NAFLD activity score.

Discussion

Collectively, our findings demonstrate that thermoneutral housing has broad but divergent effects on hepatic immune cell inflammation and hepatocellular damage across existing experimental NAFLD models in mice. These insights may serve as a foundation for future mechanistic interrogations focused on immune cell function in shaping NAFLD progression.
Altered lipid metabolites accelerate early dysfunction of T cells in HIV-infected rapid progressors by impairing mitochondrial function
Authors: Li Si-Yao , Yin Lin-Bo , Ding Hai-Bo , Liu Mei , Lv Jun-Nan , Li Jia-Qi , Wang Jing , Tang Tian , Fu Ya-Jing , Jiang Yong-Jun , Zhang Zi-Ning , Shang Hong
Published: February 2023

The complex mechanism of immune-system damage in HIV infection is incompletely understood. HIV-infected “rapid progressors” (RPs) have severe damage to the immune system early in HIV infection, which provides a “magnified” opportunity to study the interaction between HIV and the immune system. In this study, forty-four early HIV-infected patients (documented HIV acquisition within the previous 6 months) were enrolled. By study the plasma of 23 RPs (CD4+ T-cell count < 350 cells/µl within 1 year of infection) and 21 “normal progressors” (NPs; CD4+ T-cell count > 500 cells/μl after 1 year of infection), eleven lipid metabolites were identified that could distinguish most of the RPs from NPs using an unsupervised clustering method. Among them, the long chain fatty acid eicosenoate significantly inhibited the proliferation and secretion of cytokines and induced TIM-3 expression in CD4+ and CD8+ T cells. Eicosenoate also increased levels of reactive oxygen species (ROS) and decreased oxygen consumption rate (OCR) and mitochondrial mass in T cells, indicating impairment in mitochondrial function. In addition, we found that eicosenoate induced p53 expression in T cells, and inhibition of p53 effectively decreased mitochondrial ROS in T cells. More importantly, treatment of T cells with the mitochondrial-targeting antioxidant mito-TEMPO restored eicosenoate-induced T-cell functional impairment. These data suggest that the lipid metabolite eicosenoate inhibits immune T-cell function by increasing mitochondrial ROS by inducing p53 transcription. Our results provide a new mechanism of metabolite regulation of effector T-cell function and provides a potential therapeutic target for restoring T-cell function during HIV infection.
Case report: Discovery of a de novo FAM111B pathogenic variant in a patient with an APECED-like clinical phenotype
Authors: Ferré Elise M. N. , Yu Yunting , Oikonomou Vasileios , Hilfanova Anna , Lee Chyi-Chia R. , Rosen Lindsey B. , Burbelo Peter D. , Vazquez Sara E. , Anderson Mark S. , Barocha Amisha , Heller Theo , Soldatos Ariane , Holland Steven M. , Walkiewicz Magdalena A. , Lionakis Michail S.
Published: February 2023

Introduction

Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) and poikiloderma in association with tendon contractures, myopathy, and pulmonary fibrosis (POIKTMP) are rare inherited syndromes resulting from biallelic pathogenic variants in AIRE and heterozygous pathogenic variants in FAM111B, respectively. The clinical diagnosis of APECED and POIKTMP rely on the development of two or more characteristic disease manifestations that define the corresponding syndromes. We discuss the shared and distinct clinical, radiographic, and histological features between APECED and POIKTMP presented in our patient case and describe his treatment response to azathioprine for POIKTMP-associated hepatitis, myositis, and pneumonitis.

Methods

Through informed consent and enrollment onto IRB-approved protocols (NCT01386437, NCT03206099) the patient underwent a comprehensive clinical evaluation at the NIH Clinical Center alongside exome sequencing, copy number variation analysis, autoantibody surveys, peripheral blood immunophenotyping, and salivary cytokine analyses.

Results

We report the presentation and evaluation of a 9-year-old boy who was referred to the NIH Clinical Center with an APECED-like clinical phenotype that included the classic APECED dyad of CMC and hypoparathyroidism. He was found to meet clinical diagnostic criteria for POIKTMP featuring poikiloderma, tendon contractures, myopathy, and pneumonitis, and exome sequencing revealed a de novo c.1292T>C heterozygous pathogenic variant in FAM111B but no deleterious single nucleotide variants or copy number variants in AIRE.

Discussion

This report expands upon the available genetic, clinical, autoantibody, immunological, and treatment response information on POIKTMP.
Gravity disturbance compensation for dual-axis rotary modulation inertial navigation system
Authors: Zhang Panpan , Wu Lin , Bao Lifeng , Wang Bo , Liu Hui , Li Qianqian , Wang Yong
Published: February 2023

Gravity disturbance compensation is an important technique for improving the positioning accuracy of high-precision inertial navigation systems (INS). Aiming at the current problems of the resolution of gravity compensation background field and the robustness of gravity compensation algorithm are insufficient for gravity compensation. In this study, the error and frequency characteristics of INS caused by gravity disturbances are investigated. The gravity disturbance with a spatial resolution of 1’ × 1’ from a high-precision satellite altimetry marine gravity field model is preliminarily introduced into the initial alignment and pure INS calculation to implement the gravity compensation of the dual-axis rotary modulation INS. Detailed calculation results show that the east gravity disturbance affects the north attitude, and the north gravity disturbance affects the east attitude in the initial alignment. In the pure INS calculation, the horizontal gravity disturbance causes a navigation error in the form of Schuler oscillation. The INS navigation error caused by horizontal gravity disturbance is mainly affected by its amplitude; however, the horizontal gravity disturbance accuracy from the satellite altimetry model for INS gravity compensation can be ignored in practice. In addition, for low-speed underwater vehicles, the influence of high-frequency gravity disturbance signals on the INS position shows an increasing trend. Finally, the effectiveness of the gravity compensation achieved by the horizontal gravity disturbance from the satellite altimeter model is confirmed by a dynamic shipborne test. The positioning accuracy of the rotary modulation INS is maximally improved by approximately 17.9% after the horizontal gravity disturbance is compensated simultaneously in the pure INS calculation and the initial alignment.
Functional diversity in reef fish assemblages in the Parque Nacional Sistema Arrecifal Veracruzano, Mexico: Temporal and spatial changes
Authors: Russet-Rodríguez Andy J. , Pérez-España Horacio , Payán-Alcacio Juan Á. , Peterson Mark S. , De La Cruz-Agüero Gustavo , Enríquez-García Arturo B. , Ornelas-Vargas Arelly , Cruz-Escalona Víctor H.
Published: February 2023

The Parque Nacional Sistema Arrecifal Veracruzano (hereafter called PNSAV) is the largest coral reef extension in the central region of the Gulf of Mexico. These reefs are unique since they have developed near a coastal environment that is directly influenced by the discharges of Veracruz city, the rivers located on the continental shelf, and the port of Veracruz. This study evaluates the functional diversity, in terms of richness, evenness, and divergence, of the PNSAV fish community. We were interested in quantifying any similarities or differences in functional diversity metrics when one examines reef fish assemblages on a single reef or joint reef subsystems; thus, is there a difference based on scale? A total of 297 fish assemblages were observed in seven PNSAV reefs between May 2006 and April 2021. Significant differences were found in the Functional Richness of the assemblages between subsystems, years, and reef-depth interaction, but none were found among the reefs, or between seascapes. The Functional Diversity presented annual mean values between 0.83 (sd= 0.085) and 0.90 (sd= 0.068) and did not show statistical differences between years, seascape, or reefs. In contrast, statistical differences were found between subsystems, and depth level and the seascape-depth interaction. The annual mean Functional Evenness values ranged between 0.34 (sd= 0.128) and 0.44 (sd= 0.060), and significant differences were detected between years, reef, and reefs-depth level interaction, but no difference were found between subsystems. Reef-fish diversity was greater within the north coral reef subsystem than the southern of the PNSAV. There were no overall tendencies for increased functional diversity throughout the time during this study.
Properties of graphene composite fiber seamless knitted fabric and its application in boxing training
Authors: Liu Tianyue
Published: February 2023

In boxing, the wrong training methods can easily cause physical injury, so many sports protective clothing will use new materials to reduce the impact of boxing training. The aim of this paper is to design a boxing suit based on graphene composite fiber seamless knitted fabric, so as to reduce the injury of boxers during training. In this paper, the head yarn, inner yarn and knitting structure of graphene composite fiber seamless knitted fabrics are discussed, and nine kinds of fabrics are determined by orthogonal matrix method; then, a comprehensive evaluation of the wear resistance of fabrics was proposed based on sequence entropy weight technology, and the ideal solution similarity (TOPSIS) method was used to calculate the wear resistance; finally, this paper designed the boxing protective suit according to the best combination of performance. The experimental results in this paper showed that the veil of sample No. 7 is 70D/48F graphene nylon, the inner yarn is 30D graphene spandex/70D graphene nylon covered yarn, and the fabric with the knitted structure of 1 + 3 false rib has the best comprehensive performance and the highest relative proximity, reaching 0.862. The actual wearing test proves that the comfort of anti-impact boxing suit is slightly better than that of ordinary boxing suit. The clothing designed in this paper has good anti-impact effect. Therefore, on the whole, the boxing suit with graphene composite fiber seamless knitted fabric has good protective effect and can be used for boxing training.
Baclofen as a therapeutic option for gastroesophageal reflux disease: A systematic review of clinical trials
Authors: Arabpour Erfan , Khoshdel Sina , Akhgarzad Ali , Abdi Mohammadamin , Tabatabaie Negin , Alijanzadeh Dorsa , Abdehagh Mohammad
Published: February 2023

Background

The main components of gastroesophageal reflux disease (GERD) management include a combination of medications and lifestyle modifications; Nevertheless, based on the severity of symptoms and their response to medications, other treatments could be considered. Baclofen has been demonstrated in studies to relieve GERD symptoms. The current study aimed to precisely address the effects of baclofen on the treatment of GERD and its characteristics.

Methods

A systematic search was carried out in Pubmed/Medline, Cochrane CENTRAL, Scopus, Google Scholar, Web of Science, and clinicaltrials.gov up to December 10, 2021. The search terms included baclofen, GABA agonists, GERD, and reflux.

Results

We selected 26 papers that matched the inclusion criteria after examining 727 records. Studies were classified into four categories based on the study population and reported outcomes: (1) adults, (2) children, (3) patients with gastroesophageal reflux-induced chronic cough, (4) hiatal hernia patients. The results revealed that baclofen can significantly improve reflux symptoms and pH-monitoring and manometry findings to different degrees in all four mentioned categories; although its effect on pH-monitoring parameters seems less significant than the other parameters. Mild neurological and mental status deterioration were the most reported side effects. However, side effects occurred in a portion of less than 5% of short-term users and nearly 20% of long-term users.

Conclusion

In PPI-resistant patients, a trial of adding baclofen to the PPI may be helpful. Baclofen therapies may be more beneficial for symptomatic GERD patients who also report concurrent conditions including alcohol use disorder, non-acid reflux, or obesity.

Systematic review registration

https://clinicaltrials.gov/.
Structural characteristics and diversity of the rhizosphere bacterial communities of wild Fritillaria przewalskii Maxim. in the northeastern Tibetan Plateau
Authors: Cui Zhijia , Li Ran , Li Fan , Jin Ling , Wu Haixu , Cheng Chunya , Ma Yi , Wang Zhenheng , Wang Yuanyuan
Published: February 2023

Introduction

Fritillaria przewalskii Maxim. is a Chinese endemic species with high medicinal value distributed in the northeastern part of the Tibetan Plateau. F. przewalskii root-associated rhizosphere bacterial communities shaped by soil properties may maintain the stability of soil structure and regulate F. przewalskii growth, but the rhizosphere bacterial community structure of wild F. przewalskii from natural populations is not clear.

Methods

In the current study, soil samples from 12 sites within the natural range of wild F. przewalskii were collected to investigate the compositions of bacterial communities via high-throughput sequencing of 16S rRNA genes and multivariate statistical analysis combined with soil properties and plant phenotypic characteristics.

Results

Bacterial communities varied between rhizosphere and bulk soil, and also between sites. Co-occurrence networks were more complex in rhizosphere soil (1,169 edges) than in bulk soil (676 edges). There were differences in bacterial communities between regions, including diversity and composition. Proteobacteria (26.47–37.61%), Bacteroidetes (10.53–25.22%), and Acidobacteria (10.45–23.54%) were the dominant bacteria, and all are associated with nutrient cycling. In multivariate statistical analysis, both soil properties and plant phenotypic characteristics were significantly associated with the bacterial community (p < 0.05). Soil physicochemical properties accounted for most community differences, and pH was a key factor (p < 0.01). Interestingly, when the rhizosphere soil environment remained alkaline, the C and N contents were lowest, as was the biomass of the medicinal part bulb. This might relate to the specific distribution of genera, such as Pseudonocardia, Ohtaekwangia, Flavobacterium (relative abundance >0.01), which all have significantly correlated with the biomass of F. przewalskii (p < 0.05).

Discussion

F. przewalskii is evidently averse to alkaline soil with high potassium contents, but this requires future verification. The results of the present study may provide theoretical guidance and new insights for the cultivation and domestication of F. przewalskii.
Antiproliferative activity of antimicrobial peptides and bioactive compounds from the mangrove Glutamicibacter mysorens
Authors: Karthik Yalpi , Ishwara Kalyani Manjula , Krishnappa Srinivasa , Devappa Ramakrishna , Anjali Goud Chengeshpur , Ramakrishna Krishnaveni , Wani Muneeb Ahmad , Alkafafy Mohamed , Hussen Abduljabbar Maram , Alswat Amal S. , Sayed Samy M. , Mushtaq Muntazir
Published: February 2023

The Glutamicibacter group of microbes is known for antibiotic and enzyme production. Antibiotics and enzymes produced by them are important in the control, protection, and treatment of chronic human diseases. In this study, the Glutamicibacter mysorens (G. mysorens) strain MW647910.1 was isolated from mangrove soil in the Mangalore region of India. After optimization of growth conditions for G. mysorens on starch casein agar media, the micromorphology of G. mysorens was found to be spirally coiled spore chain, each spore visualized as an elongated cylindrical hairy appearance with curved edges visualized through Field Emission Scanning Electron Microscopy (FESEM) analysis. The culture phenotype with filamentous mycelia, brown pigmentation, and ash–colored spore production was observed. The intracellular extract of G. mysorens characterized through GCMS analysis detected bioactive compounds reported for pharmacological applications. The majority of bioactive compounds identified in intracellular extract when compared to the NIST library revealed molecular weight ranging below 1kgmole−1. The Sephadex G-10 could result in 10.66 fold purification and eluted peak protein fraction showed significant anticancer activity on the prostate cancer cell line. Liquid Chromatography–Mass Spectrometry (LC–MS) analysis revealed Kinetin-9-ribose and Embinin with a molecular weight below 1 kDa. This study showed small molecular weight bioactive compounds produced from microbial origin possess dual roles, acting as antimicrobial peptides (AMPs) and anticancer peptides (ACPs). Hence, the bioactive compounds produced from microbial origin are a promising source of future therapeutics.