bluuebunny/crossref_metadata_2025 huggingface dataset:
Dolor pélvico crónico en mujeres: una revisión sistemática de estudios de la prevalencia poblacional 2004-2009
Authors: Campo-Arias Adalberto
Published: February 2020

Introducción: el dolor pélvico crónico (DPC) es un síndrome de alta prevalencia en mujeres de la población general y una de las causas más frecuentes de consulta a los servicios de salud. Sin embargo, no hay disponibles revisiones sistemáticas recientes de la prevalencia de DPC en la comunidad general en los últimos cinco años.Objetivo: conocer la prevalencia de DPC en mujeres de la población general en estudios publicados entre enero de 2005 y diciembre de 2009.Materiales y métodos: se realizó una revisión sistemática de artículos que informaron la prevalencia de DPC en mujeres adultas de la población general. Se incluyeron artículos que se publicaron en español, inglés o portugués, entre enero de 2005 y diciembre de 2009. Se excluyeron las revisiones sistemáticas. Se realizó la búsqueda en BIREME, EBSCO, IMBIOMED, LILACS, MEDLINE, Ovid y ProQuest. Los Descriptores en Ciencias de la Salud que se usaron fueron: dolor pélvico (crónico) ("chronic pelvic pain"; "dor pélvica"), mujeres ("women"o "female"; "mulheres"), adulto ("adult"; "adulto"), epidemiología ("epidemiology"; "epidemiologia") y prevalencia ("prevalence"; "prevalência"). Se realizó un análisis cualitativo de los datos.Resultados: se encontraron 79 títulos de los cuales se incluyeron cuatro estudios de algunas comunidades de Australia, Estados Unidos, Austria y Nueva Zelanda que encontraron prevalencias de DPC entre 5,7 y 26,5%.Conclusiones: la prevalencia de DPC es variable en mujeres. Se necesitan más estudios en población general, en países desarrollados y en vía de desarrollo.
Quiste gigante de la glándula de Bartolino: reporte de caso y revisión de la literatura
Authors: Rivas-Perdomo Édgar E.
Published: February 2020

Introducción: en el presente artículo se expone el caso de una paciente que tuvo un quiste gigante de la glándula de Bartolino, el cual alcanzó un diámetro de 10 cm. La paciente presentó algunas dificultades diagnósticas y terapéuticas por lo que fue necesaria la escisión de la glándula y la posterior reconstrucción de la vulva. El diagnóstico definitivo fue establecido por anatomía patológica. El caso se presenta con el objetivo de realizar una revisión de la literatura respecto al tratamiento.Materiales y métodos: se realizó una búsqueda de la literatura relacionada con el tema en las bases de datos electrónicas PubMed, SciELO, ScienceDirect y MD Consult, utilizando las palabras clave: "glándulas de Bartolino", "quistes" y "marsupialización". Además, se revisaron referencias de libros de textos.Conclusiones: la urgencia en el tratamiento depende de los síntomas de la paciente. Un quiste asintomático podría no requerir tratamiento. Sin embargo, la no mejoría dentro de las primeras 72 horas o la evolución hacia una forma de absceso requerirá un tratamiento quirúrgico de emergencia. Se ha descrito una amplia variedad de tratamientos que incluyen una serie de procedimientos quirúrgicos tales como: 1) incisión simple y drenaje, 2) fistulización o marsupialización, 3) colocación de un catéter de Word, 4) escleroterapia con alcohol, 5) aplicación de nitrato de plata y 6)ablación del quiste utilizando dióxido de carbono (CO2 ) con láser.
Leiomioma uterino en paciente con síndrome de Mayer-Rokitansky-Küster-Hauser: reporte de caso
Authors: González Mariño Mario Arturo , Pérez-Solano Mario
Published: February 2020

Objetivo: el síndrome de Mayer-Rokitansky-Küster-Hauser (MRKH) es una malformación congénita del útero y la parte superior de la vagina en las mujeres que muestran características sexuales secundarias normales y cariotipo 46 XX. Puede ocurrir aplasia completa del útero en presencia de dos cuernos rudimentarios o hipoplasia uterina simétrica o asimétrica acompañada por aplasia de uno de los dos cuernos. Es poco frecuente la aparición de tumores en los rudimentos uterinos. Este caso se presenta con el objetivo de hacer una revisión de la literatura respecto a la asociación de leiomioma uterino y síndrome de MRKH.Materiales y métodos: se presenta el caso y se realiza búsqueda a través de MEDLINE en la página de PubMed con los términos "leiomyoma" y "Mayer-Rokitansky-Küster-Hauser syndrome". Se obtuvieron 8 referencias, de las cuales 7 se identificaron como pertinentes y se incluyeron en la revisión. Además, se hace una búsqueda manual de publicaciones relacionadas con la patología de base.Conclusión: el hallazgo de un leiomioma en el remanente uterino en el síndrome de MRKH es muy poco frecuente, con apenas unas cuantas publicaciones en la literatura médica. En esta paciente, con la impresión diagnóstica de leiomioma uterino, se prefirió realizar laparotomía dado el tamaño del tumor, donde se reportó la patología de biopsia por congelación y la definitiva como leiomioma.
AN IMPROVED PRE-FORECASTING ANALYSIS OF ELECTRICAL LOADS OF PUMPING STATION
Authors: Voloshko Anatolii V. , Bederak Yaroslav S. , Kozlovskyi Oleksandr A.
Published: February 2020

Relevance of research. In order to reduce energy losses, an accurate and timely forecast of the amount of consumed electricity is necessary. Accurate forecasting of electrical loads of industrial enterprises and their divisions (productions, workshops,  departments etc.) allows planning of normal operating conditions, concluding contracts for the electricity supply with the electricity supply company under more favorable conditions, and improving the electricity quality, which ultimately affects the final cost of the products produced by an enterprise. So far, more than 150 forecasting methods of electrical loads have been developed. Usually, the most convenient one is selected based on the forecaster experience by creating and analyzing several forecasting models in order to identify the best. Therefore, in order to simplify the forecasting procedure, it is necessary to develop the methodology for forecasting analysis. This methodology should enable canceling forecasting algorithms that will create lower quality forecasts. The main objective is to develop the methodology for making a forecasting analysis of power consumption on the example of a pumping station of an enterprise with a continuous cycle of work to increase the efficiency of energy consumption and implementation of energy-saving measures. Objects of research: the process of forecasting electrical loads of a pumping station of the enterprise with a continuous cycle of work. Methods of research: fundamental principles of the theory of electrical engineering, statistical methods for power consumption forecasting, the method for detecting the trend of radio signals, and fractal analysis of time series. Research results. The methodology for forecasting analysis of power consumption, which makes it possible to apply the most appropriate methods to forecast the operational power consumption, is developed. For the first time, the radio signal trend detection method is applied to identify the trend of electrical loads. The variation ranges of the fractal parameters of time series of electrical loads are established depending on the variation coefficient of the time series for different periods of time. The Brown method of exponential smoothing that is used to forecast the electrical loads, in the case of identifying the smoothing constant α is in the beyond set ( ), is further improved. The regularity of changes in the fractal parameters of time series of power consumption of a pumping station with an increase in the time series duration and their field of application are explained.
СОВРЕМЕННЫЕ СОЦИАЛЬНО ОРИЕНТИРОВАННЫЕ ПРАКТИКИ И КОНСТРУИРОВАНИЕ ИДЕНТИЧНОСТИ: ОБЛАСТИ ВЗАИМОДЕЙСТВИЯ
Authors: Гиниятова Елена Владимировна
Published: February 2020

Актуальность статьи связана с проблемой конструирования идентичности современного человека. Кризис идентичности как явление, зародившееся во второй половине ХХ в., только обостряется и усиливается в современном культурном пространстве, чему способствуют новые научные направления (например, квир-исследования). В то же время можно декларировать смену общества потребления на культуру просьюмеризма. В связи с этим изменяются и подходы к формированию идентичности. Цель: рассмотреть, как современные социально ориентированные практики культуры просьюмеризма влияют на конструирование идентичности современного человека. Выводы. Социальное предпринимательство и hhррычвааand-made как практики, получившие распространение в просьюмерской культуре, становятся формами работы с идентичностью человека и могут способствовать его самоопределению и самоидентификации.
Marco regulatorio del manejo del picudo del algodonero (Anthonomus grandis Boheman) en Colombia
Authors: Sierra-Monroy Janeth Alexandra , Burbano-Figueroa Oscar
Published: February 2020

Las medidas de control de las plagas que afectan el cultivo del algodón en Colombia han sido reguladas legalmente por más de 70 años, mucho antes que ICA, la agencia gubernamental hoy en día encargada de tal control, haya sido creada. El picudo es uno de los ejemplos más emblemáticos de la lucha contra las plagas en la agricultura colombiana, y también el ejemplo claro de las dificultades ambientales, y socioeconómicas de la implementación de medidas efectivas de control en el contexto colombiano. El control del picudo entonces es un ejemplo para revisar cómo estas normativas han cambiado a través del tiempo y analizar cómo estas han sido aplicadas para el control efectivo de esta plaga. Esta revisión además propone alternativas a los problemas actuales de regulación del picudo del algodonero en Colombia.
Effectiveness of Physical Activity Related to Food Addiction in Obese and Overweight Healthy Adults: A Systematic Review
Authors: Piñol-Piñol David , Sole Silvia , Rubí-Carnacea Francesc , Bravo Cristina
Published: February 2020

Obesity is one of the main causes of morbidity and mortality, and food addiction has been considered one of its phenotypes. The study aims to determine the effectiveness of physical activity (PA), exercise, and sedentary behaviour management in attenuating food addiction in obese and overweight healthy adults. A search strategy was undertaken using MEDLINE, CENTRAL, PsycINFO, and CINAHL until April 2019. A total of 295 studies were found from which seven studies, after duplicates were removed, were included. The acute effect of PA of moderate intensity had an effect on attentional bias, craving, and sweet taste perception, although no difference was observed between genders and body mass index groups. No effect was observed on the hedonic and reward value. The acute effects of PA of moderate intensity may be a useful tool to control the levels of food addiction. More research is needed to clarify if this effect also occurs during chronic application, at different doses and types of PA, and for all populations.
Dissipation of Momenergy to a Bose Gas by an Electromagnetic Propulsion Device
Authors: Cornwall Remi
Published: February 2020

Theoretical analysis into the energetics of a novel putative electromagnetic field propulsion device by the author, found that it was able to impart momenergy to the ground state of the electromagnetic field; some rest-energy of the craft was converted to kinetic energy of the craft. Electrical analysis showed that the propulsor was always a net electrical load – if the device accelerated from one frame, then deaccelerated to the original frame, both processes would consume electrical work. The aim of this paper is to look further into this sinking of high-grade electrical energy into the field ground state and to show that an even more pernicious form of 2nd Law of Thermodynamics exists.
Políticas de Nanotecnologia em Argentina, Brasil e México: emulação e adaptação
Authors: Invernizzi Noela
Published: February 2020

O artigo examina, de forma comparativa, o desenho e a implementação das políticas de nanotecnologia na Argentina, Brasil e México, de 2000 a 2016. A análise situa-se no contexto da recorrente emulação de políticas de ciência, tecnologia e inovação, e as adaptações realizadas durante a implementação. As dimensões analisadas se baseiam no Survey sobre Nanotecnologia, realizado em 2008 pela OECD, para facilitar a comparação internacional. Estas incluem: desenho da política, instrumentos e financiamento, atores envolvidos na governança, avaliação de questões sociais e éticas e riscos. A pesquisa foi desenvolvida mediante revisão de literatura e análise documental. Os resultados evidenciam a emulação da racionalidade e dos instrumentos das políticas dos países industrializados. Porém, a forma de governança e a avaliação das implicações sociais e dos riscos da nanotecnologia foram aspectos não emulados evidenciando processos de edição local.
Estado, Identidade e Educação: cento e cinquenta anos de resistências e lutas em Cuba
Authors: Leite Maria do Carmo Luiz Caldas , Caldas Camilo Onoda Luiz
Published: February 2020

O presente artigo está dirigido ao estudo das transformações, que abarcam o projeto societário e tendem a caminhar em conjunto com os contextos históricos em Cuba. Apresentam-se aspectos do tensionamento entre a insurgência e a invasão estrangeira, desde a dominação espanhola, passando pela república mediatizada, até a presente conjuntura de mudanças, período em que a chamada geração histórica está deixando os cargos diretivos. A discussão tem os seus alicerces fincados na visão retrospectiva dos dilemas e das tensões presentes na construção da cubanía, que vem delineando pautas à população em todas as épocas. As análises são traçadas em sentido contrário à visão eurocentrista, que recorta os esforços de compreensão relacionados às revoluções cubanas, incluindo as inconclusas e as vitoriosas dos séculos XIX e XX, abarcando um período de 150 anos, em que as raízes da cubanidade foram materializadas. As principais evidências deste exercício reflexivo, apontam que a história da Ilha produziu uma cultura de características autóctones com reflexos no sistema educativo e que as instituições escolares, afetadas por dificuldades de várias ordens, ainda assim, se apresentam como a pedra angular do processo revolucionário cubano, que tem na Educação a grande igualadora social do país.
The Central-Splitting Approach for Achilles Insertional Tendinopathy and Haglund Deformity
Authors: Ricci A. Gianni , Stewart Matthew , Thompson David , Watson Benjamin Collier , Ashmyan Roman
Published: February 2020

Background:

Insertional Achilles tendinopathy causes posterior heel pain at the insertion of the Achilles tendon, often in combination with a calcaneal exostosis, or Haglund deformity. Insertional Achilles tendinopathy often presents with a posterior osseous prominence and leads to calcification of the Achilles tendon
1
. Nonoperative treatment of these conditions includes activity modification, nonsteroidal anti-inflammatory agents, heel lifts, shoe modification, physical therapy focused on eccentric strengthening exercises, iontophoresis, and shock wave therapy. Nonoperative treatment will fail in approximately 50% of these cases, and such patients become candidates for surgical intervention
2,3
. Multiple surgical approaches have been described, including the medial J-shaped, lateral, Cincinnati transverse, double incision, and central-splitting approaches
4
. Currently, there is no consensus regarding the ideal approach. Recent literature has suggested that the central-splitting approach allows for adequate exposure of both the most commonly diseased area of the tendon and the calcaneal exostosis, with excellent postoperative pain and functional results
5–13
.

Description:

Place the patient in the prone position with the feet at the edge of the operating table. Make a full-thickness, 5 to 7-cm longitudinal incision centered over the Achilles tendon and the posterior aspect of the calcaneus. Make a central incision through the Achilles tendon. Sharply mobilize the medial and lateral slips and excise the diseased portion of the Achilles tendon. Expose the calcaneal exostosis and perform the calcaneal exostectomy with a microsagittal saw. Repair the remaining healthy-appearing Achilles tendon to the calcaneus with 2 suture anchors. An additional suture anchor or, alternatively, the double-row technique for the Achilles tendon repair may be used. Repair the central split in the Achilles tendon with absorbable suture. Close the soft tissue and skin in layers.

Alternatives:

Alternative approaches include the medial, lateral, or Cincinnati transverse incisions. The central-splitting approach is favored because of the excellent exposure of both the diseased tendon and the calcaneal exostosis. Additional augmentations to this procedure include a flexor hallucis longus transfer and a gastrocnemius recession.

Rationale:

This technique provides adequate exposure to the diseased Achilles tendon, calcific deposits, and calcaneal exostosis. Recent studies have demonstrated it to be a safe and effective technique with high patient-satisfaction scores
5–13
.
El leprosario de José Revueltas
Authors: Martínez Torres José
Published: February 2020

Este artículo se enfoca en analizar ciertos aspectos técnicos de lo que José Revueltas denominó «realismo dialéctico-materialista», que consistía en mostrar las escenas y los personajes de mayor crudeza de la sociedad desde la perspectiva de un militante comunista. Esta poética la expuso en un breve ensayo que aparece como prefacio de la edición de 1961 de Los muros de agua y que el autor consideraba el aniversario número veinte de su carrera de escritor, ya que este, su primer libro, había aparecido en 1941. Se ejemplifican los principios literarios aludidos con algunos pasajes de la misma novela y se aprovecha una idea de Virginia Woolf a propósito de la enfermedad en la literatura.
A Holistic Approach in Establishing a Structured Framework to Maximize Production Availability through Integrated ESP Key Performance Indicators
Authors: Al-Khalaf Ahmed , Alquwizani Saud , AlSadah Hashim , Flippin Cleavant
Published: February 2020

Managing large-scale electrical submersible pump (ESP) operations and assessing their performance can be a challenging task. Diverse operational environments, widely spread geographical areas, large ESP populations, and different service providers are some of the complications facing operators. Nonetheless, it is vital to the success of any artificial lift project to establish a performance evaluation structure that can effectively capture deficiencies and highlight improvements.

While many operators focus on run life statistics as the central key performance indicator (KPI) for ESPs, these types of statistics may not be sufficient in providing meaningful information to decision makers. Other important ESP performance parameters include ESP shutdowns (both planned and unplanned), restart time of tripped ESPs, commissioning time, failure rate, and the number of premature ESP failures. Thus, a comprehensive study was jointly initiated between an oil operator and ESP vendors to establish KPIs that drive improvements in all aspects. The selected KPIs were developed in a structure that ultimately focuses on maximizing production availability and revenue generation.

By constructing the ESP KPI framework, subpar performance areas were clearly visible by both the operator and the service provider. Decision makers were able to identify and act on fields that lag in performance while exerting efforts to improve underperforming service providers. Furthermore, regular meetings were conducted to review the established KPIs and recommend some action items, which might focus on either technical or operational solutions. Finally, KPI targets were set on the basis of the review of historical trends and were assigned to be challenging yet relevant and attainable. The followed practice can prove to be successful in forming a common ground where service providers can quantify losses by the operator as a result of ESP performance deficiencies.

Comprehensive data collection and keeping of ESP trips, failures, and replacements are critical to the success of this work. Regular review of field reports and well performance are imperative to accurately compute the various KPI formulas. In fact, many of the KPI calculations can be automated to capitalize on the available communication networks installed in the field to improve ESP monitoring and accurately assess their performance.
Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata
Authors: Zemanová Veronika , Popov Marek , Pavlíková Daniela , Kotrba Pavel , Hnilička František , Česká Jana , Pavlík Milan
Published: February 2020

Background Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc -Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity. Results The As accumulation of Pc -Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg -1 for 122 days, decreased with age. Content of As was higher in young than old fronds. The highest As content was determined in old fronds of Pc -Al grown in pots with 250 mg As kg -1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc -Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in both fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn. Conclusions The results of this paper point to changes in the metabolism of Pc -Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of As hyperaccumulator Pc -Al, especially in old fronds.
Multiple association analysis of loci and candidate genes that regulate body size at three growth stages in Simmental beef cattle
Authors: An Bingxing , Xu Lei , Xia Jiangwei , Wang Xiaoqiao , Miao Jian , Chang Tianpeng , Song Meihua , Ni JunQing , Xu Lingyang , Zhang Lupei , Li Junya , Gao Huijiang
Published: February 2020

Background: Body size traits as one of the main breeding selection criteria was widely used to monitor cattle growth and to evaluate the selection response. In this study, body size was defined as body height (BH), body length (BL), hip height (HH), heart size (HS), abdominal size (AS), and cannon bone size (CS). We performed genome-wide association studies (GWAS) of these traits over the course of three growth stages (6, 12 and 18 months after birth) using three statistical models, single-trait GWAS, multi-trait GWAS and LONG-GWAS. The Illumina Bovine HD 770K BeadChip was used to identify genomic single nucleotide polymorphisms (SNPs) in 1217 individuals.
Results: In total, 19, 29, and 10 significant SNPs were identified by the three models, respectively. Among these, 21 genes were promising candidate genes, including SOX2, SNRPD1, RASGEF1B, EFNA5, PTBP1, SNX9, SV2C, PKDCC, SYNDIG1, AKR1E2, and PRIM2 identified by single-trait analysis; SLC37A1, LAP3, PCDH7, MANEA, and LHCGR identified by multi-trait analysis; and P2RY1, MPZL1, LINGO2, CMIP, and WSCD1 identified by LONG-GWAS.
Conclusions: Multiple association analysis was performed for six growth traits at each growth stage. These findings offer valuable insights for the further investigation of potential genetic mechanism of growth traits in Simmental beef cattle.
The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype
Authors: Li Gang , Shi Weiyi , Niu Wenbin , Xu Jiawei , Guo Yihong , Su Yingchun , Sun Yingpu
Published: February 2020

Background: Balanced complex chromosome rearrangements (BCCR) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population.The objective is to explore the difference of influence of three types of BCCR on early embryonic development and molecular karyotype.Results:Twelve couples were recruited including four couples of three-way rearrangements carriers (group A), three couples of double two-way translocations carriers (group B) and five couples of exceptional CCR carriers (group C). A total of 243 oocytes were retrievedin the seventeen preimplantation genetic testing (PGT) cycles, and 207 of these were available for fertilization.After intracytoplasmic sperm injection, 181oocytes normally fertilized. The rates of embryos forming on day3 in three groups were 87.88%, 97.78% and77.14%, which was significantly different (P=0.01). Compared with group B, the rate of embryo formation was statistically significantly lower in group C(P=0.01).Furthermore, the rates of high-quality blastocysts in three group were 14.71%, 48.15% and 62.96%, respectively, which was significantly different (P=0.00). Compared with group B andC, the rate of high-quality blastocysts in group A was statistically significantly lower (P=0.00;P=0.00).Comprehensive chromosome analysis was performed on 83 embryos, including 75 trophectodermcellsand 8 blastomeres. Except 7 embryos failed to amplify, 9.01%embryos were diagnosed as euploidy, and 90.91% were diagnosed as abnormal. As for group A, the euploid embryo rate was 10.71%and the abnormal embryo rate was 89.29%. In group B,the euploid embryo rate was 3.85%, the abnormal embryo rate was 96.15%. The euploid embryo rate was 13.04%, the abnormal embryo rate was 86.96% in group C.There were no significant differences among the three groups (P = 0.55).Conclusions:The lowest rate of high quality blastocysts has been for three-way rearrangements and the lowest rate of euploidy has been for double two-way translocations, although no significant difference. Different types of BCCR maybe have little effect on the embryonic molecular karyotype. The difference of influence of BCCR on early embryonic developmentandmolecular karyotypeshould be further studied.
Psychiatric disorders in children and adolescents in a middle-income Latin American country
Authors: Caqueo-Urízar Alejandra , Flores Jerome , Escobar Carolang , Urzúa Alfonso , Irarrázaval Matías
Published: February 2020

Background: Child and adolescent mental health has scarcely been studied in developing countries, though it is an important aspect of health. Mental health problems in youth often continue into adulthood if not diagnosed or treated in time.Methods: The Sistema de Evaluación de Niños y Adolescentes (SENA) [Child and Adolescent Evaluation System] was used to evaluate mental health indicators in a sample of students in Northern Chile. Two age-appropriate versions of the assessment were applied to a total sample of 5,043 students, which included an elementary education sample of 1,953 schoolchildren from fourth grade through sixth grade (ages 8 to 13 years), and a Secondary School sample of 3,090 schoolchildren from seventh grade (the last year of elementary school) through the last year of Secondary school (senior high school) (ages 12 to 19 years). For each group, the version of the assessment used was determined by the students’ grade level. Both samples included municipal, government-subsidized, and private schools.Results: In this student population, depression, anxiety, and behavioral disorders were the main mental health problems identified, and indicators revealed a progressive increase in cases over the years, coinciding with the global epidemiological scenario. Males showed a greater presence of externalizing behaviors related to mental health problems associated with aggression and defiant behavior. However, females showed the highest number of mental health issues overall, especially regarding problems related to internalization. There are significant differences between school types.Conclusions: Our sample population analysis indicates that early intervention is necessary for the diagnosis and treatment of youth, with the goal of reducing the probability that psychiatric disorders will be prolonged, evolving, and worsening in adulthood.
Psychometric Properties of Instrumented Postural Sway Measures Recorded in Community Settings in Independent Living Older Adults
Authors: Alqahtani Bader , Sparto Patrick J. , Whitney Susan L. , Greenspan Susan L. , Perera Subashan , Brach Jennifer S.
Published: February 2020

Background: In the last few decades, research related to balance in older adults has been conducted in lab-based settings. The lack of portability and high cost that is associated with the current gold standard methods to quantify body balance limits their application to community settings such as independent living facilities. The purpose of the study was to examine the relative and absolute reliability and the convergent validity of static standing balance performance using an accelerometer device .
Methods: A total of 131 participants (85% female, mean age 80 ± 8 years) were included for the validity aim, and a subsample of 38 participants were enrolled in the reliability testing (89% female, mean age 76 ± 7 years). The root-mean-square (RMS) and normalized path length (NPL) for sway in antero-posterior (AP) and medio-lateral (ML) directions were calculated for different standing balance conditions. Test-retest reliability was assessed over two testing visits occurring one week apart using the intraclass correlation coefficient (ICC) for relative reliability, and the minimal detectable change (MDC) was calculated for the absolute reliability. Spearman’s rank correlation coefficient was used to test convergent validity at baseline between balance measurements and related mobility measures.
Results: Reliability of balance performance using accelerometers was good to excellent with ICC values ranging from 0.41 to 0.83 for RMS sway and from 0.49 to 0.82 for NPL sway. However, the ICC during semi-tandem stance in A-P direction was 0.35, indicating poor reliability. The MDC of the sway measurements ranged from 2.4 to 9.4 for the RMS and 5.2 to 13.8 for the NPL. Balance measurements were correlated with mobility measurements.
Conclusions: Using a portable accelerometer to quantify static standing postural control provides reliable measurements in community settings.
Factors Related to Health Civic Engagement: Results from the 2018 National Survey of Health Attitudes to Understand Progress towards a Culture of Health﻿
Authors: Dubowitz Tamara , Nelson Christopher , Weilant Sarah , Sloan Jennifer C. , Bogart Andy , Miller Carolyn , Chandra Anita
Published: February 2020

Objectives: Civic engagement, including voting, volunteering, and participating in civic organizations, is associated with better psychological, physical and behavioral health and well-being. In addition, civic engagement is increasingly viewed (e.g., in Robert Wood Johnson Foundation’s Culture of Health action framework) as a potentially important driver for raising awareness of and addressing unhealthy conditions in communities. As such, it is important to understand the factors that may promote civic engagement, with a particular focus on the less-understood, health civic engagement, or civic engagement in health-related and health-specific activities. Using data from a nationally representative sample of adults in the United States (U.S.), we examined whether the extent to which individuals feel they belong in their community (i.e., perceived sense of community) and the value they placed on investing in community health were associated with individuals’ health civic engagement.Methods: Using data collected on 7187 nationally representative respondents from the 2018 National Survey of Health Attitudes, we examined associations between sense of community, valued investment in community health, and perceived barriers to taking action to invest in community health, with health civic engagement. We constructed continuous scales for each of these constructs and employed multiple linear regressions adjusting for multiple covariates including U.S. region and city size of residence, educational attainment, family income, race/ethnicity, household size, employment status, and years living in the community.Results: Participants who endorsed (i.e., responded with mostly or completely) all 16 sense of community scale items endorsed an average of 22.8% (95%CI: 19.8 - 25.7%) more of the health civic engagement scale items compared with respondents who did not endorse any of the sense of community items. Those who endorsed (responded that it was an important or top priority) all items capturing valued investment in community health endorsed 14.0% (95%CI: 11.2 – 16.8%) more of the health civic engagement items than those who did not endorse any valued investment in community health items.Conclusions: Health civic engagement, including voting and volunteering to ultimately guide government decisions about health issues, may help improve conditions that influence health and well-being for all. Focusing on individuals’ sense of community and highlighting investments in community health may concurrently be associated with increased health civic engagement and improved community and population health.Trial Registration: Not applicable
HPV-based cervical cancer screening in Nicaragua: from testing to treatment
Authors: Holme Francesca , Maldonado Francisco , Martinez-Granera Orlando B. , Rodriguez Jose Maria , Almendarez Juan , Slavkovsky Rose , Bansil Pooja , Thomson Kerry A. , Jeronimo Jose , de Sanjose Silvia
Published: February 2020

Background: In Nicaragua, cervical cancer is the leading cause of cancer death among women. Human papillomavirus (HPV) testing, primarily using self-sampling, was introduced between 2014 and 2018 in three provinces. We analyzed data from the HPV screening program with the goal of describing key characteristics including reach, HPV prevalence, triage and treatment, and factors associated with follow-up completion. Methods: We analyzed individual-level data from routinely collected forms for women attending HPV-based cervical cancer screening. HPV-positive women were triaged with Pap or visual inspection with acetic acid (VIA) prior to treatment. Logistic regression was used to identify factors associated with receiving triage and treatment; analyses were adjusted for province, age, and self- vs. provider-collected sampling.Results: 44,635 women were screened with HPV testing; 96.6% of women used self-sampling. 6,776 women were HPV positive (15.2%), 54.0% of screen-positive women received triage, and 53.1% of triage-positive women were treated, primarily with cryotherapy. If women lost at triage are included, the overall treatment percentage was 27.8%. Province and provider sampling were significantly associated with completing triage. Province and triage type were significantly associated with receiving treatment. The odds of receiving treatment after Pap triage as compared to VIA was significantly lower (aOR: 0.05, 95% CI: 0.04–0.08, p<0.001), and the relative proportion of women receiving treatment after Pap triage versus VIA was 0.29.. Conclusions: Introduction of HPV testing resulted in a substantial number of women screened, and acceptance of self-sampling was high. Management of screen-positive women remained a challenge, particularly with Pap triage. Our results can inform other developing countries as they work to reach World Health Organization (WHO) elimination targets.Trial registration :Not applicable.
Evolutionary significance of amino acid permease transporters in 17 plants from Chlorophyta to Angiospermae
Authors: Author) Chao Zhang(Former Corresponding , Kong Nana , Cao Minxuan , Wang Dongdong , Chen Yue , Author) Qin Chen(New Corresponding
Published: February 2020

Background: Nitrogen is an indispensable nutrient for plant growth. It is used and transported in the form of amino acids in living organisms. Transporting amino acids to various parts of plants requires relevant transport proteins, such as amino acid permeases ( AAP s), which were our focus in this study. Results: We found that 5 AAP genes were present in Chlorophyte species and more AAP genes were predicted in Bryophyta and Lycophytes. Two main groups were defined and group I comprised 5 clades. Our phylogenetic analysis indicated that the origin of clades 2, 3, and 4 is Gymnospermae and that these clades are closely related. The members of clade 1 included Chlorophyta to Gymnospermae. Group II, as a new branch consisting of non-seed plants, is first proposed in our research. Our results also indicated that the AAP family was already present in Chlorophyta and then expanded accompanying the development of vasculature. Concurrently, the AAP family experienced multiple duplication events that promoted the generation of new functions and differentiation of sub-functions. Conclusions: Our findings suggest that the AAP gene originated in Chlorophyta, and some non-seed AAP genes clustered in one group. A second group, which contained plants of all evolutionary stages, indicated the evolution of AAP s. These new findings can be used to guide future research.
Efficient identification of patients eligible for clinical studies using case-based reasoning on Scottish Health Research Register (SHARE).
Authors: Shi Wen , Kelsey Tom , Sullivan Frank
Published: February 2020

Background: Trials often struggle to achieve their target sample size with only half doing so. Some researchers have turned to Electronic Health Records (EHRs), seeking a more efficient way of recruitment. The Scottish Health Research Register (SHARE) obtained patients’ consent for their EHRs to be used as a searching base from which researchers can find potential participants. However, due to the fact that EHR data is not complete, sufficient or accurate, a database search strategy may not generate the best case-finding result. The current study aims to evaluate the performance of a case-based reasoning method in identifying participants for population-based clinical studies recruiting through SHARE, and assess the difference between its resultant cohort and the original one deriving from searching EHRs. Methods: A case-based reasoning framework was applied to 119 participants in nine projects using two-fold cross-validation, with records from a further 86,292 individuals used for testing. A prediction score for study participation was derived from the diagnosis, procedure, pharmaceutical prescription, and laboratory test results attributes of each participant. Evaluation was conducted by calculating Area Under the ROC Curve and information retrieval metrics for the ranking list of the test set by prediction score. We compared the most likely participants as identified by searching a database to those ranked highest by our model. Results: The average ROCAUC for nine projects was 81% indicating strong predictive ability for these data. However, the derived ranking lists showed lower predictive performance, with only 21% of the persons ranked within top 50 positions being the same as identified by searching databases. Conclusions: Case-based reasoning is may be more effective than a database search strategy for participant identification for clinical studies using population EHRs. The lower performance of ranking lists derived from case-based reasoning means that patients identified as highly suitable for study participation may still not be recruited. This suggests that further study is needed into improvements in the collection and curation of population EHRs, such as use of free text data to aid reliable identification of people more likely to be recruited to clinical trials.
PTH Derivative Promotes Wound Healing via Synergistic Multicellular Stimulating and Exosomal Activities
Authors: Shen Yi-Fan , Huang Jing-Huan , Wang Kai-Yang , Zheng Jin , Cai Lin , Gao Hong , Li Xiao-Lin , Li Jing-Feng
Published: February 2020

Background: Diabetic wounds are a disturbing and rapidly growing clinical problem. A novel peptide, parathyroid hormone related peptide (PTHrP-2), is assumed as multifunctional factor in angiogenesis, fibrogenesis and re-epithelization. This study aims to test PTHrP-2 efficiency and mechanism in wound healing. Methods: Through repair phenomenon in vivo some problems were detected, and further research on their mechanisms was made. In vivo therapeutic effects of PTHrP-2 were determined by HE, Masson, microfil and immunohistochemical staining. In vitro direct effects of PTHrP-2 were determined by proliferation, migration, Vascular Endothelial Grown Factor and collagen I secretion of cells and Akt/ Erk1/2 pathway change. In vitro indirect effects of PTHrP-2 was study via exosomes. Exosomes from PTHrP-2 untreated and treated HUVECs and HFF-1 cells were insolated and identified. Exosomes were co-cultured with original cells, HUVECs or HFF-1 cells, and epithelial cells. Proliferation and migration and pathway change were observed. PTHrP-2-HUVEC-Exos were added into in vivo wound to testify its hub role in PTHrP-2 indirect effects in wound healing. Results: In vivo, PTHrP-2 exerted multifunctional pro-angiogenesis, pro-firbogenesis and re-epithelization effects. In vitro, PTHrP-2 promoted proliferation and migration of endothelial and fibroblast cells, but had no effect on epithelial cells. Therefore, we tested PTHrP-2 indirect effects via exosomes. PTHrP-2 intensified intercellular communication between endothelial cells and fibroblasts and initiated endothelial-epithelial intercellular communication. PTHrP-2-HUVEC-Exos played a hub role in PTHrP-2 indirect effects in wound healing. Conclusion: These findings of this study indicated that PTHrP-2, a multifunctional factor, could promote wound healing via synergistic multicellular stimulating and exosomal activities. Key words PTH, multifunctional factor, diabetic wound, exosomes, synergistic effect
Optical monitoring of glutamate release at multiple synapses in situ detects changes following LTP induction
Authors: Kopach Olga , Zheng Kaiyu , Rusakov Dmitri
Published: February 2020

Information processing and memory formation in the brain relies on release of the main excitatory neurotransmitter glutamate from presynaptic axonal specialisations. The classical Hebbian paradigm of synaptic memory, long-term potentiation (LTP) of transmission, has been widely associated with an increase in the postsynaptic receptor current. Whether and to what degree LTP induction also enhances presynaptic glutamate release has been the subject of debate. Here, we took advantage of the recently developed genetically encoded optical sensors of glutamate (iGluSnFR) to monitor its release at CA3-CA1 synapses in acute hippocampal slices, before and after the induction of LTP. We attempted to trace release events at multiple synapses simultaneously, by using two-photon excitation imaging in fast frame-scanning mode. We thus detected a significant increase in the average iGluSnFR signal during potentiation, which lasted for up to 90 min. This increase may reflect an increased amount of released glutamate or, alternatively, reduced glutamate binding to high-affinity glutamate transporters that compete with iGluSnFR.
Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice
Authors: Lei Han , Xie Bowen , Gao Tong , Cen Qianhong , Ren Yi
Published: February 2020

Background Existing methods for preparing influenza vaccines pose the greatest challenge against highly pandemic avian influenza H7N9 outbreak in the poultry and humans. Exploring a new strategy for manufacturing and delivering a safe and effective H7N9 vaccine is needed urgently. Results An alternative approach is to develop an influenza H7N9 oral vaccine based on yeast display technology in a timely manner. Hemagglutinin (HA) of A/Anhui/1/2013 (AH-H7N9) is used as a model antigen and characterized its expression on the surface of Saccharomyces cerevisiae (S.cerevisiae) EBY 100. Mice administrated orally with S.cerevisiae EBY100/pYD5-HA produced significant titers of IgG antibody as well as significant amounts of cytokines IFN-γ and IL-4. Importantly, S.cerevisiae EBY100/pYD5-HA could provide effective immune protection against homologous A/Anhui/1/2013 (AH-H7N9) virus challenge. Conclusions Our findings suggest that platform based on yeast surface technology provides an alternative approach to prepare a promising influenza H7N9 oral vaccine candidate that can significantly shorten the preparedness period and result in effective protection against influenza A pandemic. Keywords: S.cerevisiae EBY100/pYD5-HA, Yeast display technology, Influenza A pandemic.
Decreased Angiogenic And Increased Apoptotic Activities Of Bone Microvascular Endothelial Cells In Patients With Glucocorticoid-Induced Osteonecrosis Of The Femoral Head
Authors: Yu Huachen , Liu Pei , Zuo Wei , Sun Xiaowei , Liu Hongzhi , Lu Feifan , Guo Wanshou , Zhang Qidong
Published: February 2020

Background: Glucocorticoid-induced osteonecrosis of the femoral head (ONFH) is closely associated with the dysfunction of the bone microvascular endothelial cells (BMECs). The present study investigated the angiogenic and apoptotic activity of the BMECs in glucocorticoid-induced ONFH. Methods: This study enrolled a total of 12 patients, six of whom were assigned to the ONFH group whereas the other six served as the control group. The ONFH group was composed of patients with glucocorticoid-induced ONFH while the control group had femoral neck fractures. BMECs were isolated from the subchondral region of the femoral head. Cell proliferation, cell viability, tube formation assay, Transwell assay, TUNEL assay, and Western blot analysis were performed. Results: BMECs of the two groups were successfully isolated and identified. No significant differences were noticed in BMECs proliferation between the two groups. However, compared to the control, cell viability, tube formation, and migration of BMECs were significantly decreased and the number of TUNEL positive cells was markedly increased in the ONFH group. In the ONFH group, it was also noted that the amount of Bax and cleaved-caspase3 was elevated while that of Bcl-2 was reduced. Conclusion: The findings of our study revealed that BMECs obtained from the glucocorticoid-induced ONFH patients had decreased angiogenic and increased apoptotic activities, which could explain the pathogenesis and progression of glucocorticoid-induced ONFH.