bluuebunny/crossref_metadata_2025 huggingface dataset:
Classification and Characterization of Traumatic Brain Injuries in the Northern Region of Sweden
Authors: Magnusson Beatrice M. , Koskinen Lars-Owe D.
Published: December 2023

Background: Traumatic brain injury (TBI) is a common cause of death and disability, the incidence of which in northern Sweden is not fully investigated. This study classifies and characterize epidemiological and demographic features of TBIs in a defined population in Umeå county, Sweden. Specifically, to evaluate frequencies of (1) intracranial lesions detected with computed tomography (CT), (2) need for emergency intervention, and (3) hospital admission, in minimal, mild, moderate, and severe TBI, respectively. Methods: The data were gathered from 4057 TBI patients visiting our emergency room (ER) during a two-year period (2015–2016), of whom 56% were men and approximately 95% had minimal TBIs (Glasgow Coma Scale (GCS), score 15). Results: Of all injuries, 97.8% were mild (GCS 14–15), 1.7% were moderate (GCS 9–13), and 0.5% were severe (GCS < 9). CT scans were performed on 46% of the patients, with 28% being hospitalized. A high annual TBI incidence of 1350 cases per 100,000 citizens was found. The mortality rate was 0.5% with the majority as expected in the elderly group (>80 years). Conclusions: Minimal TBIs were not as mild as previously reported, with a relatively high frequency of abnormal CT findings and a high mortality rate. No emergency intervention was required in patients in the GCS 13–15 group with normal CT scans. These findings have implications for clinical practice in the ER with the suggestion to include biomarkers to reduce unnecessary CT scans.
Post-Transjugular Intrahepatic Portosystemic Shunt (TIPS) Hepatic Encephalopathy—A Review of the Past Decade’s Literature Focusing on Incidence, Risk Factors, and Prophylaxis
Authors: Friis Karina Holm , Thomsen Karen Louise , Laleman Wim , Montagnese Sara , Vilstrup Hendrik , Lauridsen Mette Munk
Published: December 2023

Transjugular intrahepatic portosystemic shunt (TIPS) is an established treatment for portal hypertension and its’ complications in liver cirrhosis, yet the development of hepatic encephalopathy (HE) remains a significant concern. This review covers the reported incidence, risk factors, and management strategies for post-TIPS HE over the past decade. Incidence varies widely (7–61%), with factors like age, liver function, hyponatremia, and spontaneous portosystemic shunts influencing risk. Procedural aspects, including TIPS timing, indication, and stent characteristics, also contribute. Pharmacological prophylaxis with lactulose and rifaximin shows promise, but current evidence is inconclusive. Procedural preventive measures, such as shunt embolization and monitoring portal pressure gradients, are explored. Treatment involves pharmacological options like lactulose and rifaximin, and procedural interventions like stent diameter reduction. Ongoing studies on novel predictive markers and emerging treatments, such as faecal microbiota transplant, reflect the evolving landscape in post-TIPS HE management. This concise review provides clinicians with insights into the multifaceted nature of post-TIPS HE, aiding in improved risk assessment, prophylaxis, and management for patients undergoing TIPS procedures.
Exchange of a Tracheal Tube and Supraglottic Airway Device: Evaluation of Different Techniques in Three Simulated Airway Scenarios (TUBE Study)—A Prospective, Randomised Controlled Study
Authors: Kriege Marc , Demare Tim , Ruemmler Robert , Schmidtmann Irene , Wojciechowski Janosh , Busch Anneke , Ott Thomas
Published: December 2023

Background: The swapping of a supraglottic airway device or a tracheal tube in anaesthetised adult patients is a challenging procedure because potential complications through hypoxemia and loss of airway may occur, with life-threatening implications. This study aims to evaluate which airway technique offers the highest success rate concerning a secure airway in established supraglottic airway and tracheal tube airway exchange scenarios. Methods: After ethical approval, anaesthesiologists were randomised 1:1 into simulated scenarios: an LTS group (malpositioned laryngeal tube) and a Cuff group (relevant cuff leakage of a placed tracheal tube). After that, both groups completed a common scenario consisting of a partially obstructed tracheal tube lumen in a fixed prone position with a Mayfield clamp. The primary endpoint was a successful tracheal airway exchange within ten minutes after the start of the scenario and before severe hypoxemia (SpO2 < 80%) arose. Secondary endpoints were the evaluation of factors influencing success after 10 min. Results: In total, 60 anaesthesiologists (LTS group n = 30; Cuff group n = 30) with a median experience of 7 years (IQR 4-11) were observed. Within 10 min, a malpositioned laryngeal tube was successfully exchanged by 27/30 (90%) participants, compared to the exchange of a tracheal tube with a relevant cuff leakage by 29/30 (97%; p > 0.05). An airway exchange in an obstructed tube scenario occurred in 22/59 (37%). Loss of airway maintenance showed an obvious association with failure in the common scenario (p = 0.02). Conclusion: The results of this simulation-based study reflect that the exchange of an existing but insufficient airway device in clinical practice is a high-risk procedure. Especially in a fixed prone position, the deliberate evaluation of the existing airway patency and well-conceived airway management in the case of the accidental loss of the airway or obstructed airway access are crucial.
Blood-Based Biomarkers Reflecting Protease 3 and MMP-12 Catalyzed Elastin Degradation as Potential Noninvasive Surrogate Markers of Endoscopic and Clinical Disease in Inflammatory Bowel Disease
Authors: Pehrsson Martin , Domislovic Viktor , Alexdottir Marta Sorokina , Brinar Marko , Karsdal Morten Asser , Barisic Ana , Krznaric Zeljko , Mortensen Joachim Høg
Published: December 2023

Chronic inflammation in inflammatory bowel disease (IBD) triggers significant extracellular matrix remodeling, including elastin remodeling, leading to severe clinical complications. Novel methods to assess intestinal tissue destruction may act as surrogate markers of endoscopic disease activity, relieving patients of invasive endoscopy. We explored the noninvasive blood-based biomarkers ELP-3 and ELM-12, measuring elastin degradation in IBD. In a study involving 104 Crohn’s disease (CD), 39 ulcerative colitis (UC), and 29 healthy donors, we assessed these biomarkers’ association with endoscopic and clinical disease activity using ELISA. Patients were evaluated based on the SES-CD and CDAI for CD patients and modified MES and partial Mayo for UC patients. ELP-3 and ELM-12 were elevated in patients with IBD. Discerning CD patients in endoscopic remission and mild from moderate to severe, ELP-3 provided an AUC of 0.69 and ELM-12 an AUC of 0.73. The ELP-3 biomarker was associated with UC patients and provided the highest diagnostic power of 0.87 for remission vs. active clinical disease. The data suggest an association of ELP-3 with active CD and ELM-12 with endoscopic remission in CD patients. Additionally, ELP-3 could identify UC patients with active clinical disease from patients in remission. The noninvasive biomarkers ELP-3 and ELM-12 could be potential surrogate biomarkers of elastin degradation and endoscopic and clinical disease markers.
Using Ecological Momentary Assessments to Study How Daily Fluctuations in Psychological States Impact Stress, Well-Being, and Health
Authors: Mengelkoch Summer , Moriarity Daniel P. , Novak Anne Marie , Snyder Michael P. , Slavich George M. , Lev-Ari Shahar
Published: December 2023

Despite great interest in how dynamic fluctuations in psychological states such as mood, social safety, energy, present-focused attention, and burnout impact stress, well-being, and health, most studies examining these constructs use retrospective assessments with relatively long time-lags. Here, we discuss how ecological momentary assessments (EMAs) address methodological issues associated with retrospective reports to help reveal dynamic associations between psychological states at small timescales that are often missed in stress and health research. In addition to helping researchers characterize daily and within-day fluctuations and temporal dynamics between different health-relevant processes, EMAs can elucidate mechanisms through which interventions reduce stress and enhance well-being. EMAs can also be used to identify changes that precede critical health events, which can in turn be used to deliver ecological momentary interventions, or just-in-time interventions, to help prevent such events from occurring. To enable this work, we provide examples of scales and single-item questions used in EMA studies, recommend study designs and statistical approaches that capitalize on EMA data, and discuss limitations of EMA methods. In doing so, we aim to demonstrate how, when used carefully, EMA methods are well poised to greatly advance our understanding of how intrapersonal dynamics affect stress levels, well-being, and human health.
Reusing Bisphenol—A Type of Epoxy Polymer Recyclates from the Solvolysis of CFRP
Authors: Cho Ching Mui , Wang Xiaobai , Tsumura Sean Kenzo , Thitsartarn Warintorn , Tay Siok Wei
Published: December 2023

Carbon fiber-reinforced polymer (CFRP) composites are highly functional composites which comprise two major components: the polymer matrix and the carbon fiber. Lightweight and having high strength, CFRPs have been used heavily in various industries such as wind, aerospace and automobile. The increasing demand and extensive use led to a huge quantum of CFRP waste from both end-of-life and during manufacturing. Out of this waste, only 2% is recycled, the rest are disposed of via incineration and/or landfill. This has raised significant environmental and sustainability concerns. The current state-of-the-art way of recycling CFRPs is by pyrolysis. However, through the pyrolysis process, the polymer used in the CFRPs, which accounts for around 65–75 wt.%, cannot be recovered and reused. In most publications, the focus on CFRP recycling was on the recovering of the more valuable carbon fiber. The polymer matrix is mostly burnt off, in the case of pyrolysis, or disposed. To obtain full circularity, recovering and reusing both the carbon fiber and polymer is necessary. In this paper, we primarily focus on the recovered bisphenol-A type of epoxy polymer (REP) obtained from solvolysis digestion of CFRP and explore the feasibility of reusing this REP by blending it with pristine epoxy in various compositions to create new materials. The physical and mechanical properties, including decomposition temperatures (Td), glass transition temperatures (Tg), storage modulus, loss modulus, flexural and tensile strength, were characterized using thermal gravity analyzer (TGA), differential scanning calorimetry (DSC), dynamic mechanical analyzer (DMA) and Instron universal tester. The results indicate a decrease in glass transition and decomposition temperature, and mechanical properties as the blending composition increases. This suggests that the total blending composition should not exceed 10 wt.%, with an optimal range potentially falling between 5 to 6 wt.%.
Sacrificial-Rotating Rod-Based 3D Bioprinting Technique for the Development of an In Vitro Cardiovascular Model
Authors: Lee Jooyoung , Lee Hyungseok
Published: December 2023

Several studies have attempted to develop complex cardiovascular models, but the use of multiple cell types and poor cell alignments after fabrication have limited the practical application of these models. Among various bioprinting methods, extrusion-based bioprinting is the most widely used in the bioengineering field. This method not only has the potential to construct complex 3D biological structures but it also enables the alignment of cells in the printing direction owing to the application of shear stress to the cells during the printing process. Therefore, this study developed an in vitro cardiovascular model using an extrusion-based bioprinting method that utilizes a rotating rod as a printing platform. The rotating rod was made of polyvinyl alcohol (PVA) and used as a sacrificial rod. This rotating platform approach enabled the printing of longer tubular-vascular structures of multiple shapes, including disease models, and the water-soluble properties of PVA facilitated the isolation of the printed vascular models. In addition, this method enabled the printing of the endothelial cells in the bloodstream direction and smooth muscle cells in the circumferential direction to better mimic the anatomy of real blood vessels. Consequently, a cardiovascular model was successfully printed using a gelatin methacryloyl bioink with cells. In conclusion, the proposed fabrication method can facilitate the fabrication of various cardiovascular models that mimic the alignment of real blood vessels.
Design Methodology of Wind Turbine Rotor Models Based on Aerodynamic Thrust and Torque Equivalence
Authors: Ma Yuan , Chen Chaohe , Yin Guang , Ong Muk Chen , Lu Hongchao , Fan Tianhui
Published: December 2023

Limited by scaling effects, the physical model tests of FOWTs (floating offshore wind turbines) cannot simulate the aerodynamic loads on rotors correctly. To solve this problem, the real-time hybrid model tests in wind tunnels were developed and provided a feasible solution for the aerodynamic simulation. To perform the wind tunnel tests, the design of aerodynamic equivalent rotor models is most critical. In this study, an innovative methodology of aerodynamic equivalent design for the wind turbine rotors is developed based on GA (genetic algorithm). The NREL (National Renewable Energy Laboratory) 5 MW and DTU (Technical University of Denmark) 10 MW rotors are employed for the case studies to validate the proposed methodology. According to the results, the model-scale aerodynamic thrust performance can be accurately matched with the prototype in the entire region between cut-in and cut-out wind speeds, which allows the rotor model to provide correct thrust at different wind speeds. The variance of the aerodynamic torque with the wind speeds for the developed model is also in good agreement with the prototype, which could be beneficial for the design of the model-scale active pitch control strategy. Moreover, the applicability of the fitness functions of GA is discussed.
A Numerical Simulation Study and Effectiveness Evaluation on the Flow Field Effect of Trapezoidal Artificial Reefs in Different Layouts
Authors: Chen Xiaolong , Che Xuan , Zhou Yin , Tian Changfeng , Li Xinfeng
Published: December 2023

The combined release of artificial reefs in different quantities and arrangements leads to different flow field effects. This study designs a small trapezoidal artificial reef. To optimize the quantity and layout of these reefs, trapezoidal reefs in three different layouts were selected for analysis at five different velocity gradients (0.1, 0.5, 1.0, 1.5, and 2.0 m/s). The effects of disposal spacing and layout on the flow field effect of trapezoidal artificial reefs at different flow velocities were simulated using Ansys Fluent. According to the findings: after simulation, flow velocity could indirectly reflect the distribution of upwelling and back eddy, the scale and strength of upwelling increased as flow velocity increased, and the back eddy showed no obvious variation with flow velocity. In transverse combination mode, both the scale and strength of the upwelling and back eddy were maximized when the reef spacing was 1.0 L; in longitudinal combination mode, upwelling and back eddy reached maximum scale and strength when the disposal spacing of the reefs was 1.5 L of a single reef. In 2020, flow mapping and fishery surveys were carried out in the engineering pilot area. The results showed that the number and species of fish populations with 1.5 L spacing in the vertical combination method were significantly higher than those in other forms, and the structure of the fish reef was stable without any flipping or sliding phenomenon. This study can provide a theoretical reference for the design and the actual deployment of artificial reefs to improve the ecological restoration of the water.
Integrating Software FMEA and STPA to Develop a Bayesian Network-Based Software Risk Model for Autonomous Ships
Authors: Yang Xue , Zhu Yawei , Zhou Tao , Xu Sheng , Zhang Wenjun , Zhou Xiangyu , Meng Xiangkun
Published: December 2023

The autonomous shipping industry is increasingly focusing on enhancing the safety and reliability of software-based systems. Conducting a risk assessment is a requirement for demonstrating the safety equivalence of autonomous ships based on such systems to conventional vessels. Traditional risk assessment models, however, primarily focus on hardware failures, often overlooking potential software-related failures and functional inadequacies. This study proposes a framework integrating Software Failure Mode and Effects Analysis (FMEA), System–Theoretic Process Analysis (STPA), and Bayesian Network (BN) for risk identification of autonomous ship software systems. The results of a case study reveal that the framework sufficiently addresses the multifaceted nature of risks related to software in autonomous ships. Based on the findings of this study, we suggest the need for standardization of software architecture development in the autonomous ship industry and highlight the necessity for an enhanced understanding of AI-specific risks and the development of tailored risk assessment methodologies.
Effect of Lateral Airflow on Initial HSI and Flame Behavior of Marine Fuel in a Ship Engine Room: Experiment and Analysis
Authors: Wang Kan , Ming Yang , Liu Xiaolei , Wang Hao , He Yuru
Published: December 2023

The flame behavior of engine fires, such as those caused by leaked fuel coming into contact with an ignition source, is significant in practical applications, where flame detection is used to minimize the damage of the attendant ship fire safety problem. In this work, the flame behavior of hot-surface ignition (HSI) under crossflow was studied, with a particular focus on the difference in lateral airflow velocities for HSI-driven flame deviations at the windward and leeward sides of a ship engine room; a problem such as this has not previously been quantified. Full-scale experiments were conducted in a ship engine room using marine diesel and hydraulic oil as the fuel, and by adopting lateral airflow with the velocities of 0 m/s, 1.0 m/s, 3.0 m/s, and 5.0 m/s, together with an HSI mechanism consisting of marine diesel and hydraulic oil coming into contact with elevated hot-surface temperatures. The results show that the effects of disturbing the combustible gaseous mixture for marine fuel HSI, at both the windward and leeward sides, strengthened as the airflow velocity increased. The HSI position of the leaked marine fuel in the engine room was strongly dependent on ventilation, while that under the airflow condition decreased with the increase in the hot-surface temperature. A model was proposed to characterize this difference on the basis of the HSI height, which was defined as the ratio of the height during the initial HSI to the stationary period. The results indicate that the scale of the flame gradually increased in the horizontal direction, which was significantly different from the result in the scenario without mechanical ventilation. The results also revealed that the fluctuation of hydraulic oil through the temperature field was significant and lasted for a long time under a low HSI temperature.
An Underwater Image Restoration Deep Learning Network Combining Attention Mechanism and Brightness Adjustment
Authors: Zheng Jianhua , Zhao Ruolin , Yang Gaolin , Liu Shuangyin , Zhang Zihao , Fu Yusha , Lu Junde
Published: December 2023

This study proposes Combining Attention and Brightness Adjustment Network (CABA-Net), a deep learning network for underwater image restoration, to address the issues of underwater image color-cast, low brightness, and low contrast. The proposed approach achieves a multi-branch ambient light estimation by extracting the features of different levels of underwater images to achieve accurate estimates of the ambient light. Additionally, an encoder-decoder transmission map estimation module is designed to combine spatial attention structures that can extract the different layers of underwater images’ spatial features to achieve accurate transmission map estimates. Then, the transmission map and precisely predicted ambient light were included in the underwater image formation model to achieve a preliminary restoration of underwater images. HSV brightness adjustment was conducted by combining the channel and spatial attention to the initial underwater image to complete the final underwater image restoration. Experimental results on the Underwater Image Enhancement Benchmark (UIEB) and Real-world Underwater Image Enhancement (RUIE) datasets show excellent performance of the proposed method in subjective comparisons and objective assessments. Furthermore, several ablation studies are conducted to understand the effect of each network component and prove the effectiveness of the suggested approach.
Dermatophyte Infections Worldwide: Increase in Incidence and Associated Antifungal Resistance
Authors: Kruithoff Caroline , Gamal Ahmed , McCormick Thomas S. , Ghannoum Mahmoud A.
Published: December 2023

The increase in incidence of superficial fungal infections combined with the emergence of antifungal resistance represents both a global health challenge and a considerable economic burden. Recently, dermatophytes, the main culprit causing superficial fungal infections, have started to exhibit antifungal resistance. This can be observed in some of the most common species such as Trichophyton rubrum and Trichophyton mentagrophytes. Importantly, the new subspecies, known as Trichophyton indotineae, has been reported to show high resistance to terbinafine, a first-line treatment for dermatophyte infections. Compounding these issues is the realization that diagnosing the causative infectious agents requires using molecular analysis that goes beyond the conventional macroscopic and microscopic methods. These findings emphasize the importance of conducting antifungal susceptibility testing to select the appropriate antifungal necessary for successful treatment. Implementing these changes may improve clinical practices that combat resistant dermatophyte infections.
Comparison of the Effect of BPA and Related Bisphenols on Membrane Integrity, Mitochondrial Activity, and Steroidogenesis of H295R Cells In Vitro
Authors: Štefunková Nikola , Greifová Hana , Jambor Tomáš , Tokárová Katarína , Zuščíková Lucia , Bažány Denis , Massányi Peter , Capcarová Marcela , Lukáč Norbert
Published: December 2023

Bisphenol A (BPA) is an endocrine-disruptive chemical that is widely utilized in the production of polycarbonate plastic and epoxy resin, which are used to make a wide range of consumer products, food and drink containers, and medical equipment. When the potential risk of BPA emerged, it was substituted by allegedly less harmful substitutes such as bisphenols S, F, B, and AF. However, evidence suggests that all bisphenols can have endocrine-disruptive effects, while the extent of these effects is unknown. This study aimed to determine effect of BPA, BPAF, BPB, BPF, and BPS on viability and steroidogenesis in human adrenocortical carcinoma cell line in vitro. The cytotoxicity of bisphenols was shown to be considerable at higher doses. However, at low concentrations, it improved viability as well as steroid hormone secretion, indicating that bisphenols have a biphasic, hormetic effect in biological systems. The results are consistent with the hypothesis that bisphenols selectively inhibit some steroidogenic enzymes. These findings suggest that bisphenols have the potential to disrupt cellular steroidogenesis in humans, but substantially more detailed and systematic research is needed to gain a better understanding of the risks associated with bisphenols and their endocrine-disrupting effect on humans and wildlife.
A Control Architecture for Developing Reactive Hybrid Remotely Operated Underwater Vehicles
Authors: Gómez-Bravo Fernando , Garrocho-Cruz Alejandro , Marín-Cañas Olga , Pulido-Calvo Inmaculada , Gutierrez-Estrada Juan Carlos , Peregrín-Rubio Antonio
Published: December 2023

This article introduces a control architecture designed for the development of Hybrid Remotely Operated Underwater Vehicles. The term ”Hybrid” characterizes Remotely Operated systems capable of autonomously executing specific operations. The presented architecture maintains teleoperation capabilities while enabling two fully autonomous applications. The approach emphasizes the implementation of reactive navigation by exclusively utilizing data from a Mechanical Scanned Imaging Sonar for control decisions. This mandates the control system to solely react to data derived from the vehicle’s environment, without considering other positioning information or state estimation. The study involves transforming a small-scale commercial Remotely Operated Underwater Vehicle into a hybrid system without structural modifications, and details the development of an intermediate Operational Control Layer responsible for sensor data processing and task execution control. Two practical applications, inspired by tasks common in natural or open-water aquaculture farms, are explored: one for conducting transects, facilitating monitoring and maintenance operations, and another for navigating toward an object for inspection purposes. Experimental results validate the feasibility and effectiveness of the authors’ hypotheses. This approach expands the potential applications of underwater vehicles and facilitates the development of Hybrid Remotely Operated Underwater Vehicles, enabling the execution of autonomous reactive tasks.
New Spectral Results for Laplacian Harary Matrix and the Harary Laplacian-Energy-like Applying a Matrix Order Reduction
Authors: Medina Luis , Rodríguez Jonnathan , Trigo Macarena
Published: December 2023

In this paper, we introduce the concepts of Harary Laplacian-energy-like for a simple undirected and connected graph G with order n. We also establish novel matrix results in this regard. Furthermore, by employing matrix order reduction techniques, we derive upper and lower bounds utilizing existing graph invariants and vertex connectivity. Finally, we characterize the graphs that achieve the aforementioned bounds by considering the generalized join operation of graphs.
New Ways to Calculate the Probability in the Bertrand Problem
Authors: Rodrigo Javier , López Mariló , Lantarón Sagrario
Published: December 2023

We give two new ways of calculating the probability of a chord of circumference randomly selected being larger than the side of an equilateral triangle inscribed in the circumference (this problem is known as the Bertrand paradox). The first one employs an immersion in R4, and the second one uses a direct probability measure over the set of chords.
A Fuzzy Entropy-Based Group Consensus Measure for Financial Investments
Authors: Dombi József , Fáró Jenő , Jónás Tamás
Published: December 2023

This study presents a novel, fuzzy entropy-based approach to the measurement of consensus in group decision making. Here, the basic assumption is that the decision inputs are the ‘yes’ or ‘no’ votes of group members on a financial investment that has a particular expected rate of return. In this paper, using a class of fuzzy entropies, a novel consensus measure satisfying reasonable requirements is introduced for a case where the decision inputs are dichotomous variables. It is also shown here that some existing consensus measures are just special cases of the proposed fuzzy entropy-based consensus measure when the input variables are dichotomous. Next, the so-called group consensus map for financial investments is presented. It is demonstrated that this construction can be used to characterize the level of consensus among the members of a group concerning financial investments as a function of the expected rate of return. Moreover, it is described how a consensus map can be constructed from empirical data and how this map is connected with behavioral economics.
Global Existence, Blowup, and Asymptotic Behavior for a Kirchhoff-Type Parabolic Problem Involving the Fractional Laplacian with Logarithmic Term
Authors: Guan Zihao , Pan Ning
Published: December 2023

In this paper, we studied a class of semilinear pseudo-parabolic equations of the Kirchhoff type involving the fractional Laplacian with logarithmic nonlinearity: ut+M([u]s2)(−Δ)su+(−Δ)sut=|u|p−2uln|u|,in Ω×(0,T),u(x,0)=u0(x),in Ω,u(x,t)=0,on ∂Ω×(0,T),, where [u]s is the Gagliardo semi-norm of u, (−Δ)s is the fractional Laplacian, s∈(0,1), 2λ
2s, and u0 is the initial function. To start with, we combined the potential well theory and Galerkin method to prove the existence of global solutions. Finally, we introduced the concavity method and some special inequalities to discuss the blowup and asymptotic properties of the above problem and obtained the upper and lower bounds on the blowup at the sublevel and initial level.
The Impact of Comorbidities and Demographic Factors on Ejection Fraction
Authors: Soysaler Cezara Andreea , Andrei Cătălina Liliana , Ceban Octavian , Sinescu Crina Julieta
Published: December 2023

Heart failure (HF) presents an increasingly significant problem as the population ages. The cause of HF plays a significant role in determining treatment options and outcomes. It is worth noting that several studies have identified gender disparities in both morbidity and mortality, which may suggest differing causes of HF. The purpose of this research is to investigate the influence of various factors, including demographics and comorbidities, on ejection fraction (EF). The objectives of this study involve implementing preventive measures, ensuring timely diagnosis, and implementing interventions that target risk factors and specific comorbidities. These efforts aim to improve the prognosis for individuals affected by heart failure. The main method consists of linear regression. The demographic factors under scrutiny are gender and education, while the comorbidities of interest encompass valvulopathy, ischemia, smoking, obesity, high cholesterol, and diabetes. The main results consist of the fact that high education is associated with a 12.8% better EF on average, while among the factors with a negative role analyzed, ischemia is the most harmful, being 12.8% lower on average. Factors with a smaller impact are smoking, obesity, and high cholesterol. Diabetes does not seem to affect EF.
Study on the Pharmacological Mechanism of Icariin for the Treatment of Alzheimer’s Disease Based on Network Pharmacology and Molecular Docking Techniques
Authors: Wang Dongwei , Zheng Jilong , Sun Xingsheng , Xie Liuwei , Yang Yang
Published: December 2023

The purpose of this study is to explore the pharmacological mechanism of icariin (ICA) in the treatment of Alzheimer’s disease (AD) based on network pharmacology and network molecular docking technology. In order to investigate the regulatory effect of ICA on the expression level of AD pathological phosphorylation regulatory proteins, this study further explored the possible molecular mechanism of ICA regulating AD autophagy through network pharmacology. Macromolecular docking network was verified by Autodock Vina 1.1.2 software. The main active ingredients of ICA, the physicochemical properties, and pharmacokinetic information of ICA were predicted using online databases and relevant information. The results showed that the targets of MAPK3, AKT1, HSP90AA1, ESR1, and HSP90AA1 were more critical in the treatment of AD. Autophagy, apoptosis, senescence factors, phosphatidylinositide 3-kinase/protein kinase B (P13K/AKT) signaling pathway, MAKP, mTOR, and other pathways were significantly associated with AD. Docking of ICA with HIF-1, BNIP3, PINK1, and Parkin pathway molecules showed that the key targets of the signaling pathway were more stably bound to ICA, which may provide a better pathway for ICA to regulate autophagy by providing a better pathway. ICA can improve AD, and its mechanism may be related to the P13K/AKT, MAKP, and mTOR signaling pathways, thereby regulating autophagy-related proteins.
Direct Impact of the Air on Mutant Cells for Mutagenicity Assessments in Urban Environments
Authors: Caredda Chiara , Franchitti Elena , Gilli Giorgio , Pignata Cristina , Traversi Deborah
Published: December 2023

Background: Urban air pollution is recognized as a critical problem for public health and is classified as a carcinogen for humans. A great number of studies have focused on the monitoring of urban air mutagenicity. One of the best-known and applied methods for assessing mutagenicity is the Ames test, a bacterial reverse mutation test. The classic protocol for assessing air mutagenicity involves the concentration of particulate matter (PM) on filters and subsequent extraction using organic solvents. This work aimed to develop a method for the evaluation of air mutagenicity directly impacted by air on microbial plates already containing an Ames’ microbial sensor. Methods: A specific six-month sampling campaign was carried out in Turin in a period with high air pollution. Samples were tested for mutagenicity on Salmonella typhimurium strains TA98, TA100, and YG1024 with the traditional method and with the new direct method. Results: The new protocol is able to evaluate the mutagenicity of the sampled air and obtain repeatable results. The final sensitivity is similar to the traditional method (≈10 net revertants/m3); however, the mutagenic response is due to the complete air pollution mixture, including volatile and semivolatile pollutants avoiding the concentration of filters and the following laborious extraction procedures. Conclusions. Despite some critical issues in contamination control, the method is easier, faster, and less expensive than traditional methods.
Mosquito-Borne Arboviruses Occurrence and Distribution in the Last Three Decades in Central Africa: A Systematic Literature Review
Authors: Poungou Natacha , Sevidzem Silas Lendzele , Koumba Aubin Armel , Koumba Christophe Roland Zinga , Mbehang Phillipe , Onanga Richard , Zahouli Julien Zahouli Bi , Maganga Gael Darren , Djogbénou Luc Salako , Borrmann Steffen , Adegnika Ayola Akim , Becker Stefanie C. , Mavoungou Jacques François , Nguéma Rodrigue Mintsa
Published: December 2023

Arboviruses represent a real public health problem globally and in the Central African subregion in particular, which represents a high-risk zone for the emergence and re-emergence of arbovirus outbreaks. Furthermore, an updated review on the current arbovirus burden and associated mosquito vectors is lacking for this region. To contribute to filling this knowledge gap, the current study was designed with the following objectives: (i) to systematically review data on the occurrence and distribution of arboviruses and mosquito fauna; and (ii) to identify potential spillover mosquito species in the Central African region in the last 30 years. A web search enabled the documentation of 2454 articles from different online databases. The preferred reporting items for systematic reviews and meta-analyses (PRISMA) and the quality of reporting of meta-analyses (QUORUM) steps for a systematic review enabled the selection of 164 articles that fulfilled our selection criteria. Of the six arboviruses (dengue virus (DENV), chikungunya virus (CHIKV), yellow fever virus (YFV), Zika virus (ZIKV), Rift Valley fever virus (RVFV), and West Nile virus (WNV)) of public health concern studied, the most frequently reported were chikungunya and dengue. The entomological records showed >248 species of mosquitoes regrouped under 15 genera, with Anopheles (n = 100 species), Culex (n = 56 species), and Aedes (n = 52 species) having high species diversity. Three genera were rarely represented, with only one species included, namely, Orthopodomyia, Lutzia, and Verrallina, but individuals of the genera Toxorhinchites and Finlayas were not identified at the species level. We found that two Aedes species (Ae. aegypti and Ae. albopictus) colonised the same microhabitat and were involved in major epidemics of the six medically important arboviruses, and other less-frequently identified mosquito genera consisted of competent species and were associated with outbreaks of medical and zoonotic arboviruses. The present study reveals a high species richness of competent mosquito vectors that could lead to the spillover of medically important arboviruses in the region. Although epidemiological studies were found, they were not regularly documented, and this also applies to vector competence and transmission studies. Future studies will consider unpublished information in dissertations and technical reports from different countries to allow their information to be more consistent. A regional project, entitled “Ecology of Arboviruses” (EcoVir), is underway in three countries (Gabon, Benin, and Cote d’Ivoire) to generate a more comprehensive epidemiological and entomological data on this topic.
Antibacterial, Resistance Modulation, Anti-Biofilm Formation, and Efflux Pump Inhibition Properties of Loeseneriella africana (Willd.) N. Halle (Celastraceae) Stem Extract and Its Constituents
Authors: Anokwah Daniel , Asante-Kwatia Evelyn , Asante Jonathan , Obeng-Mensah Daniel , Danquah Cynthia Amaning , Amponsah Isaac Kingsley , Ameyaw Elvis Ofori , Biney Robert Peter , Obese Ernest , Oberer Lukas , Amoako Daniel Gyamfi , Abia Akebe Luther King , Mensah Abraham Yeboah
Published: December 2023

This study investigated the antibacterial, resistance modulation, biofilm inhibition, and efflux pump inhibition potentials of Loeseneriella africana stem extract and its constituents. The antimicrobial activity was investigated by the high-throughput spot culture growth inhibition (HT-SPOTi) and broth microdilution assays. The resistance modulation activity was investigated using the anti-biofilm formation and efflux pump inhibition assays. Purification of the extract was carried out by chromatographic methods, and the isolated compounds were characterized based on nuclear magnetic resonance, Fourier transform infrared and mass spectrometry spectral data and comparison with published literature. The whole extract, methanol, ethyl acetate, and pet-ether fractions of L. africana all showed antibacterial activity against the test bacteria with MICs ranging from 62.5 to 500.0 µg/mL The whole extract demonstrated resistance modulation effect through strong biofilm inhibition and efflux pump inhibition activities against S. aureus ATCC 25923, E. coli ATCC 25922 and P. aeruginosa ATCC 27853. Chromatographic fractionation of the ethyl acetate fraction resulted in the isolation of a triterpenoid (4S,4αS,6αR,6βS,8αS,12αS,12βR,14αS,14βR)-4,4α,6β,8α,11,11,12β,14α-Octamethyloctadecahydropicene-1,3(2H,4H)-dione) and a phytosterol (β-sitosterol). These compounds showed antibacterial activity against susceptible bacteria at a MIC range of 31–125 µg/mL and potentiated the antibacterial activity of amoxicillin (at ¼ MIC of compounds) against E. coli and P. aeruginosa with modulation factors of 32 and 10, respectively. These compounds also demonstrated good anti-biofilm formation effect at a concentration range of 3–100 µg/mL, and bacterial efflux pump inhibition activity at ½ MIC and ¼ MIC against E. coli and P. aeruginosa. Loeseneriella africana stem bark extracts and constituents elicit considerable antibacterial, resistance modulation, and biofilm and efflux pump inhibition activities. The results justify the indigenous uses of L. africana for managing microbial infections.
Barley Yellow Dwarf Virus Influences Its Vector’s Endosymbionts but Not Its Thermotolerance
Authors: Chirgwin Evatt , Yang Qiong , Umina Paul A. , Thia Joshua A. , Gill Alex , Song Wei , Gu Xinyue , Ross Perran A. , Wei Shu-Jun , Hoffmann Ary A.
Published: December 2023

The barley yellow dwarf virus (BYDV) of cereals is thought to substantially increase the high-temperature tolerance of its aphid vector, Rhopalosiphum padi, which may enhance its transmission efficiency. This is based on experiments with North American strains of BYDV and R. padi. Here, we independently test these by measuring the temperature tolerance, via Critical Thermal Maximum (CTmax) and knockdown time, of Australian R. padi infected with a local BYDV isolate. We further consider the interaction between BYDV transmission, the primary endosymbiont of R. padi (Buchnera aphidicola), and a transinfected secondary endosymbiont (Rickettsiella viridis) which reduces the thermotolerance of other aphid species. We failed to find an increase in tolerance to high temperatures in BYDV-infected aphids or an impact of Rickettsiella on thermotolerance. However, BYDV interacted with R. padi endosymbionts in unexpected ways, suppressing the density of Buchnera and Rickettsiella. BYDV density was also fourfold higher in Rickettsiella-infected aphids. Our findings indicate that BYDV does not necessarily increase the temperature tolerance of the aphid transmission vector to increase its transmission potential, at least for the genotype combinations tested here. The interactions between BYDV and Rickettsiella suggest new ways in which aphid endosymbionts may influence how BYDV spreads, which needs further testing in a field context.
Remobilization of HFSE, Y, and REE during Diagenetic Alteration of Heavy Minerals in Sandstones from the Chvalčov Site, Flysch Belt of the Outer Western Carpathians, Czech Republic
Authors: Dolníček Zdeněk , Krejčí Kotlánová Michaela , Ulmanová Jana , Sejkora Jiří
Published: December 2023

An in situ electron microprobe study of detrital minerals yielded important insights into the diagenetic history of the Cretaceous-to-Paleogene flysch sandstones from the Chvalčov site, Rača Unit, Flysch Belt of the Outer Western Carpathians. Detrital titanite and a Fe-Ti mineral (probably ilmenite) were almost completely altered to TiO2 minerals, which also newly crystallized in intergranular spaces of sandstone. Brookite, anatase, and, exceptionally, rutile were identified by Raman spectroscopy. Authigenic TiO2 phases show complex composition with occasionally elevated contents of Fe, Nb, Zr, V, Sc, Cr, Al, Y, and/or P, which were likely sourced from altered neighboring heavy minerals. In addition, rare authigenic LREE- and Y-enriched apatite rims were observed on detrital apatite. The remobilization of REE, Y, and HFSE was likely mediated by acidic early diagenetic fluids enriched in fluoride and sulfate anions. The superimposed formation of calcite cement was associated with the dissolution of detrital garnet, feldspars, and quartz. The compositions of detrital apatite and garnet (Alm60-82Prp4-30Sps0-24Grs0-19) are comparable with those from adjacent parts of the Flysch Belt. Detrital rutile is enriched in Nb, V, Cr, and Zr. Our study illustrates the intensity of diagenetic alteration of detrital minerals in flysch sandstones as well as the usefulness of in-situ electron-microprobe investigations for the recognition of processes influencing heavy minerals in diagenetically altered sediments.
Reworking of Eoarchean to Mesoarchean Continental Crust in the Anshan–Benxi Area, North China Craton—Evidence from Lianshanguan ca. 2.5 Ga Syenogranites
Authors: Zhang Wen , Wang Wei , Du Lilin , Liu Pinghua , Xu Wang
Published: December 2023

The Anshan–Benxi area, situated in the northeast of the North China Craton (NCC), is home to not only the oldest rocks in China (~3.8 Ga) but also a diverse range of granitoids dated between 3.8 and 2.5 Ga. The Lianshanguan batholith, covering an area of approximately 250 km2 with an east–west trend, predominantly consists of syenogranites (K2O > 4 wt. % and K2O/Na2O ratios > 1.3). Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) U-Pb analyses of the two syenogranites yielded concordant ages of 2541 ± 22 and 2512 ± 13 Ma, respectively. These syenogranites had zircon εHf(t) values ranging from −20 to +4.9 with two-stage Hf model ages (TDM2(Hf)) spanning 3.9–2.7 Ga. Based on petrological, geochemical, and isotopic characteristics, we conclude that the Lianshanguan syenogranites are mainly resulted from the reworking of complicated Eoarchean–Mesoarchean crustal materials, possibly with a small proportion of ~2.7 Ga juvenile crustal materials. When compared with coeval syenogranites from the Northern Liaoning and Western Liaoning–Eastern Hebei areas, ~2.5 Ga syenogranites from the Anshan–Benxi area displayed more complicated TDM2(Hf) ages, hinting at a pronounced late Neoarchean reworking of the Eoarchean to Mesoarchean continental crust (including metasedimentary sources) primarily in the Anshan–Benxi region of the North China Craton. This scenario significantly bolsters the arc–continent collision model.
Probing Reactivity with External Forces: The Case of Nitroacetamides in Water
Authors: La Penna Giovanni , Machetti Fabrizio
Published: December 2023

Many computational methods have been applied to interpret and predict changes in reactivity by slight modifications of a given molecular scaffold. We describe a novel and simple method based on approximate density-functional theory of valence electrons that can be applied within a large high-performance computational infrastructure to probe such changes using a statistical sample of molecular configurations, including the solvent. All the used computational tools are fully open-source. Following our previous application, we are able to explain the high acidity of C-H bond at α position in nitro compounds when the amide linkage an ammonium group is inserted into the α substituent.
Monitoring of Cleaning Treatments for Paper Heritage with Raman Spectroscopy Mapping
Authors: Botti Sabina , Bonfigli Francesca , Mezi Luca , Flora Francesco
Published: December 2023

In the field of book heritage, it is important to develop cleaning/disinfecting treatments that can slow down the degradation of paper to prevent evident and irreversible damage. The objectives of the cleaning treatments are to remove external contaminants and oxidation and decomposition products of the paper, but these processes must not modify the unique characteristics of the book heritage resulting in irreversible changes in the structure of the paper. Recently, several innovative cleaning treatments were developed with the aim of being minimally invasive; however, to assess the effect of these treatments on paper, it is necessary to use a diagnostic non-destructive, rapid, and affordable process. In previous work, we used surface scanning Raman spectroscopy to develop a diagnostic protocol able to follow the aging processes of the paper, discriminating between hydrolysis and oxidation. In this paper, we applied this protocol to study the action of different types of treatments (hydrogel and EUV irradiation), evaluating both their effectiveness and impact on paper parameters. The results reported here demonstrate that the developed in operando diagnostic procedure can follow the changes in the paper structure comparing them to the variability due to the intrinsic inhomogeneity of paper, without sample contact in a rapid and effective way.