bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of aging and oxidative/genotoxic stress on poly(ADP-ribose) polymerase-1 activity in rat brain.
Authors: Strosznajder Robert P , Jesko Henryk , Adamczyk Agata
Published: November 2019

Poly(ADP-ribose) polymerase-1 (PARP-1, EC 2.4.2.30), a DNA-bound enzyme, plays a key role in genome stability, but after overactivation can also be responsible for cell death. The aim of the present study was to investigate PARP-1 activity in the hippocampus, brain cortex, striatum and cerebellum in adult (4 months) and aged (24 months) specific pathogen free Wistar rats and to correlate it with PARP-1 protein level and p53 expression. Moreover, the response of PARP-1 in adult and aged hippocampus to oxidative/genotoxic stress was evaluated. Our data indicated a statistically significant enhancement of PARP-1 activity in aged hippocampus and cerebral cortex comparing to adults without statistically significant changes in PARP-1 protein level. The expression of p53 mRNA was elevated in all aged brain parts with the exception of the cerebral cortex. Our data suggest that enhancement of PARP-1 activity and p53 expression in aged brain may indicate higher DNA damage. Our data also indicate that during excessive oxidative/genotoxic stress there is no response of PARP-1 activity in aged hippocampus in contrast to a significant enhancement of PARP-1 activity in adults which may have important consequences for the physiology and pathology of the brain.
New spectrophotometric methods for the determination of nifedipine in pharmaceutical formulations.
Authors: Rahman Nafisur , Azmi Syed Najmul Hejaz
Published: November 2019

Two simple, sensitive and economical spectrophotometric methods were developed for the determination of nifedipine in pharmaceutical formulations. Method A is based on the reaction of the nitro group of the drug with potassium hydroxide in dimethyl sulphoxide (DMSO) medium to form a coloured product, which absorbs maximally at 430 nm. Method B uses oxidation of the drug with ammonium molybdate and subsequently reduced molybdenum blue is measured at 830 nm. Beer's law is obeyed in the concentration range of 5.0-50.0 and 2.5-45.0 microg ml(-1) with methods A and B, respectively. Both methods have been successfully applied for the assay of the drug in pharmaceutical formulations. No interference was observed from common pharmaceutical adjuvants. The reliability and the performance of the proposed methods are established by point and interval hypothesis tests and through recovery studies.
Role of NAD(P)H:quinone oxidoreductase (NQO1) in apoptosis induction by aziridinylbenzoquinones RH1 and MeDZQ.
Authors: Nemeikaite-Ceniene Ausra , Dringeliene Aldona , Sarlauskas Jonas , Cenas Narimantas
Published: November 2019

We aimed to characterize the role of NAD(P)H:quinone oxidoreductase (NQO1) in apoptosis induction by antitumour quinones RH1 (2,5-diaziridinyl-3-hydroxymethyl-6-methyl-1,4-benzoquinone) and MeDZQ (2,5-dimethyl-3,6-diaziridinyl-1,4-benzoquinone). Digitonin-permeabilized FLK cells catalyzed NADPH-dependent single- and two-electron reduction of RH1 and MeDZQ. At equitoxic concentrations, RH1 and MeDZQ induced apoptosis more efficiently than the nonalkylating duroquinone or H(2)O(2). The antioxidant N,N'-diphenyl-p-phenylene diamine, desferrioxamine, and the inhibitor of NQO1 dicumarol, protected against apoptosis induction by all compounds investigated, but to a different extent. The results of multiparameter regression analysis indicate that RH1 and MeDZQ most likely induce apoptosis via NQO1-linked formation of alkylating species but not via NQO1-linked redox cycling.
The effect of new non-cross resistant antitumour agents on the energy state of human erythrocytes.
Authors: Nowak Robert , Baranowska-Bosiacka Irena , Stefańska Barbara , Machaliński Bogusław , Hłyńczak Alina J , Tarasiuk Jolanta
Published: November 2019

Multidrug resistance (MDR) of tumour cells is related to the overexpression of ATP-dependent pumps responsible for the active efflux of antitumour agents out of resistant cells. Benzoperimidine and anthrapyridone compounds exhibit comparable cytotoxic activity against sensitive and MDR tumour cells. They diffuse extremely rapidly across the plasma membrane and render the ATP-dependent efflux inefficient. Such uptake could disturb an energy metabolism of normal cells possessing an elevated level of ATP-dependent proteins, especially erythrocytes having a high level of the MRP1, MRP4 and MRP5 proteins. In this study the effect of five antitumour agents: benzoperimidine (BP1), anthrapyridones (CO1, CO7) and reference drugs used in the clinic: doxorubicin (DOX) and pirarubicin (PIRA), on the energetic state in human erythrocytes has been examined. These compounds have various types of structure and kinetics of cellular uptake (slow--DOX, CO7, moderate--PIRA, fast--BP1, CO1) resulting in their different ability to saturate ATP-dependent transporters. The energetic state of erythrocytes was examined by determination of purine nucleotide contents (ATP, ADP, AMP), NAD(+) and values of adenylate energy charge (AEC) using an HPLC method. It was found that the level of nucleotides as well as the AEC value of erythrocytes were not changed during 24 h of incubation with these agents independently of their structure and ability to saturate ATP-dependent pumps. This is a very promising result in view of their potential use in the clinic as antitumour drugs against multidrug resistant cancers.
Chelating ability of proctolin tetrazole analogue.
Authors: Łodyga-Chruścińska Elzbieta , Sanna Daniele , Micera Giovanni , Chruściński Longin , Olejnik Jadwiga , Nachman Ronald J , Zabrocki Janusz
Published: November 2019

The aim of the investigation was to establish the chelating ability of a new proctolin analogue of the sequence Arg-Tyr-LeuPsi[CN(4)]Ala-Thr towards copper(II) ions. The insertion of the tetrazole moiety into the peptide sequence has considerably changed the coordination ability of the ligand. Potentiometric and spectroscopic (UV-Vis, CD, EPR) results indicate that the incorporation of 1,5-disubstituted tetrazole ring favours the formation of a stable complex form of CuH(-1)L. This 4N coordination type complex is the dominant species in the physiological pH range. The tetrazole moiety provides one of these nitrogens. The data indicate that Cu(II) ions are strongly trapped inside the peptide backbone. These findings suggest that Cu(II) can hold peptide chains in a bent conformation. This bent conformation may be essential for bioactivity of the tetrazole peptides.
Cyclic dermorphin tetrapeptide analogues obtained via ring-closing metathesis.
Authors: Berezowska Irena , Chung Nga N , Lemieux Carole , Wilkes Brian C , Schiller Peter W
Published: November 2019

The dermorphin-derived cyclic tetrapeptide analogues H-Tyr-c[D-Cys-Phe-Cys]NH(2) and H-Tyr-c[D-Cys-Phe-D-Cys]NH(2) are opioid agonists at the mu and delta receptor. To enhance the metabolic stability of these peptides, we replaced the disulfide bridge with a bis-methylene moiety. This was achieved by solid-phase synthesis of the linear precursor peptide containing allylglycine residues in place of the Cys residues, followed by ring-closing metathesis. In the case of the peptide with L-configuration in the 4-position both the cis and the trans isomer of the resulting olefinic peptides were formed, whereas the cis isomer only was obtained with the peptide having the D-configuration in position 4. Catalytic hydrogenation yielded the saturated -CH(2)-CH(2)- bridged peptides. In comparison with the cystine-containing parent peptides, all olefinic peptides showed significantly reduced mu and delta agonist potencies in the guinea pig ileum and mouse vas deferens assays. The -CH(2)-CH(2)-bridged peptide with l-configuration in the 4-position was equipotent with its cystine-containing parent in both assays, whereas the bis-methylene analogue with D-configuration in position 4 was 10-27-fold less potent compared to its parent. The effect of the disulfide replacements with the -CH=CH- and -CH(2)-CH(2)- moieties on the conformational behavior of these peptides was examined by theoretical conformational analysis which provided plausible explanations in terms of structural parameters for the observed changes in opioid activity.
Synthetic peptides mimicking antigenic epitope of Helicobacter pylori urease.
Authors: Białek Magdalena , Grabowski Sebastian , Kamiński Zbigniew , Kaca Wiesław
Published: November 2019

Short peptides resembling the Helicobacter pylori urease antigen (UreB F8 Ser-Ile-Lys-Glu-Asp-Val-Gln-Phe) with deleted aspartic acid and glutamic acid residues, anchored through a triazine linker via the N-terminal moiety to cellulose plate were prepared. The peptides were used for binding of antibodies from sera of patients with medically confirmed atherosclerosis. Recognition of the peptides was also tested with anti-Jack beans urease antibodies. The important role of a Gly-Gly spacer separating the peptides from the cellulose support was shown. Different patterns of binding of antibodies from H. pylori infected patients and anti-Jack bean urease antibodies were observed only in the case of pentapeptides. The peptide Gly-Gly-Leu-Val-Phe-Lys-Thr was recognized by most of the tested sera.
Dynamic force measurements of avidin-biotin and streptavdin-biotin interactions using AFM.
Authors: De Odrowaz Piramowicz Marzena , Czuba Paweł , Targosz Marta , Burda Kvetoslava , Szymoński Marek
Published: November 2019

Using atomic force microscopy (AFM) we performed dynamic force measurements of the adhesive forces in two model systems: avidin-biotin and streptavidin-biotin. In our experiments we used glutaraldehyde for immobilization of (strept)avidin on the tip and biotin on the sample surface. Such interface layers are more rigid than those usually reported in the literature for AFM studies, when (strept)avidin is coupled with biotinylated bovine albumin and biotin with agarose polymers. We determined the dependence of the rupture forces of avidin-biotin and streptavidin-biotin bonds in the range 300-9600 pN/s. The slope of a semilogarithmic plot of this relation changes at about 1700 pN/s. The existence of two different regimes indicates the presence of two activation barriers of these complexes during the dissociation process. The dissociation rates and activation energy barriers, calculated from the Bell model, for the avidin-biotin and streptavidin-biotin interactions are similar to each other for loading rates > 1700 pN/s but they are different from each other for loading rates < 1700 pN/s. In the latter case, the dissociation rates show a higher stability of the avidin-biotin complex than the streptavidin-biotin complex due to a larger outer activation barrier of 0.8 k(B)T. The bond-rupture force is about 20 pN higher for the avidin-biotin pair than for the streptavidin-biotin pair for loading rates < 1700 pN/s. These two experimental observations are in agreement with the known structural differences between the biotin binding pocket of avidin and of streptavidin.
Synaptic protein UNC-13 interacts with an F-box protein that may target it for degradation by proteasomes.
Authors: Polinsky Cristina , Houston Chanelle , Vado Jaynine , Shaikh Azizahmed , Kohn Rebecca E
Published: November 2019

UNC-13 protein participates in regulating neurotransmitter release. In Drosophila melanogaster, proteasomal degradation controls UNC-13 levels at synapses. Function of the amino-terminal region of a 207 kDa form of Caenorhabditis elegans UNC-13 is unknown. Yeast two-hybrid and secondary yeast assays identified an F-box protein that interacts with this amino-terminal region. As F-box proteins bind proteins targeted for proteasomal degradation, this protein may participate in degrading a subset of UNC-13 proteins, suggesting that different forms of UNC-13 are regulated differently. Yeast assays also identified an exonuclease, a predicted splicing factor, and a protein with coiled-coil domains, indicating that UNC-13 may affect RNA function.
Cloning of two genes encoding Rab7 in Paramecium.
Authors: Surmacz Liliana , Wiejak Jolanta , Wyroba Elzbieta
Published: November 2019

Rab7 is a small GTPase that plays a crucial role in the regulation of transport from early to late endosomes and lysosomes, phagosome maturation and in lysosomal biogenesis in mammalian cells. It contains conserved and unique sequence elements that mediate its function. Two Rab7 genes, Rab7a (703 bp) and Rab7b (707 bp) were identified in the unicellular eukaryote Paramecium by PCR amplification. They contain three short introns of different lengths (28-32 bp) and sequence located at identical positions in both genes. The presence of two Rab7 genes in the Paramecium genome was confirmed by Southern hybridization analysis performed with six different restriction enzymes. Expression of both genes was assessed by Northern blot and RT-PCR. Two transcripts of 1.8 and 2.2 kb were identified by hybridization analysis. The cloned complementary DNAs, both of 618 nucleotides in length, encode polypeptides of 206 amino acids that are 97.6% identical and differ in their C-termini. The predicted protein sequences of Rab7a and Rab7b contain all characteristic domains essential for Rab function: the effector domain (YRATVGADF) and four GTP-binding consensus sequences (GDSGVGKT, WDTAGQ, NKLD, SAK) as well as the prenylation motif (-CC) at the C-terminus indispensable for Rab binding to the membrane. Similarity searches revealed 81.6-82.1% homology of Paramecium Rab7 isoforms to human Rab7 and a lack of an insert typical for the Kinetoplastida - the species that appeared earlier in evolution. Paramecium is the first free-living lower eukaryote in which homologues of Rab7 have been identified that exhibit features similar to those of mammalian Rab7.
Nonhomologous end-joining deficiency of L5178Y-S cells is not associated with mutation in the ABCDE autophosphorylation cluster.
Authors: Brzóska Kamil , Kruszewski Marcin , Szumiel Irena
Published: November 2019

Cells with mutated autophosphorylation sites in the ABCDE cluster of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) are defective in the repair of ionising radiation-induced DSB, but show in an in vitro test the same DNA-PK activity as the cells possessing wild type enzyme. Nevertheless, the mutated DNA-PK is able to undergo ATP-dependent autophosphorylation and inactivation. This characteristics correspond well with the phenotypic features of the L5178Y-S (LY-S) cell line that is defective in DSB repair, shows a pronounced G1 phase radiosensitivity, but in which the level of DNA-PK activity present in total cell extracts is similar to that of its radioresistant counterpart L5178Y-R (LY-R) cell line. The purpose of this work was to examine the possible alterations in the sequence encoding the cluster of autophosphorylation sites in the DNA-dependent protein kinase in LY-S cells. Despite the presence of phenotypic features indicating the possibility of such alterations, no differences were found between the sequences coding for the autophosphorylation sites in L5178Y-R and L5178Y-S cells. In conclusion, the repair defect in LY-S cells is not related to the structure of the DNA-PK autophosphorylation sites (ABCDE casette).
Experience and enlightenment: “Customer-oriented” nine-stage major gifts management model of world-class U.S. universities
Authors: Lin Chenghua , Xie Yanjie , Xu Ruixue
Published: November 2019

Since the 1990s, “customer orientation” marketing has been widely applied to major gifts management of world-class U.S. universities, forming a donor-centered model for major gifts management. It focuses on donor demands management, relationship cultivation and value creation, which has a major effect on university fundraising and development. Through literature review and case study of the University of Pittsburgh, this study analyzes the major gifts management model of world-class U.S. universities in terms of theory, structure, operation model, and development characteristics. The findings show that there are two important characteristics of the organizational structure of major gifts management in the United States: one is the donation market segmentation and the donor classification management; the other is the refinement of donation management functions. And this paper proposes a nine-stage major gifts management model of world-class U.S. universities, which contains “Definition-Identification- Qualification-Development of Strategy-Cultivation-Solicitation-Negotiation-Acknowledgment -Stewardship.” Finally, this paper summarizes the development trend of major gifts management: emphasizing donor’s value creation, donor internalization and capitalization, cultivating prospective donors from national to international, moving towards integration of leading capacity building and donor orientation.Received: 08 April 2019Accepted: 05 November 2019Published online: 29 November 2019
Qualidade e enquadramento dos recursos hídricos na bacia do rio Cuiabá na região metropolitana de Cuiabá
Authors: Marchetto Margarida , Noquelli Luiz Henrique , Alves Marciely Ferreira
Published: November 2019

Se propôs neste trabalho avaliar a qualidade da água do rio Cuiabá na região mais povoada, ou seja, no perímetro urbano da Capital Mato-grossense. Essa avaliação se justifica devido a captação de água para abastecimento das cidades de Várzea Grande e Cuiabá. Objetivo foi também avaliar os usos e impactos na aplicação dos instrumentos “outorga” para as indústrias localizadas na área urbana e “enquadramento” para os diversos setores de acordo com os usos da água na bacia hidrográfica. Avaliar o principal problema que é a poluição pontual devido à falta de saneamento no perímetro urbano, dos maiores aglomerados urbanos do estado de Mato Grosso se concentram nesta bacia, como a região metropolitana de Cuiabá que engloba a capital do estado com 580 mil habitantes e a segunda maior cidade, Várzea Grande com população de 185 mil habitantes, total aproximado de 765 mil habitantes. Para alcançar o objetivo, baseou-se no diagnóstico da qualidade de água na área de estudo, por meio de pesquisa bibliográfica. Foram utilizados dados da SEMA referente as cinco estações ao longo do percurso urbano do Rio Cuiabá, um ponto a montante da cidade, um ponto a jusante e três pontos intermediários, no período entre janeiro de 2011 a novembro de 2016, abrangendo tanto o período chuvoso, quanto o período de seca. Resultados de monitoramento ambiental realizado por pesquisadores da UFMT (2019) e no diagnóstico do Plano de Bacia da Região Hidrográfica-RH do rio Paraguai (2018). Os dados referentes a demanda pelo uso da água obtida junto ao órgão gestor de recursos hídricos no estado, a qualidade da água dos corpos hídricos inseridos na bacia e uso preponderante mais restritivo, em seu perímetro urbano do município de Cuiabá. Os resultados das análises indicam certa contaminação encontrada nas águas do Rio Cuiabá, originada da região urbana do município de Várzea Grande e Cuiabá e que ela vem alterando as características físicas, químicas e biológicas da água. Essa alteração muda a classe do rio em determinados meses, no período de chuvas.
ГЕОМЕХАНИЧЕСКАЯ ОЦЕНКА ПАРАМЕТРОВ УСТОЙЧИВОСТИ ОТКОСОВ БОРТОВ И УСТУПОВ ПРИ ОТРАБОТКЕ МЕСТОРОЖДЕНИЯ АПАТИТ-НЕФЕЛИНОВЫХ РУД «ОЛЕНИЙ РУЧЕЙ»
Authors: Немова Наталья Анатольевна , Бельш Татьяна Александровна
Published: November 2019

Актуальность обусловлена необходимостью безопасного ведения горных работ на карьерах глубиной свыше 360м. Наращивание объёмов добычи возможно при значительном углублении; для снижения объёмов вскрыши необходимо укручение откосов бортов и уступов. Для решения данного вопроса необходимо знать геомеханическую обстановку уступов и бортов как на предельном контуре, так и при изменении их основных параметров – высоты и углов наклона. Цель: проанализировать горно-геологические условия месторождения «Олений ручей» и существующие данные о физико-механических свойствах горных пород, слагающих массив рассматриваемого месторождения, произвести расчет и проверку устойчивости откосов бортов карьера на предельном контуре и изыскать возможность укручения откосов бортов и вскрышных уступов при ведении горных работ. Объект: крупное месторождение апатит-нефелиновых руд «Олений ручей». Методы: горно-геологический анализ месторождения «Олений ручей» и анализ существующих данных о физико-механических свойствах горных пород, слагающих массив рассматриваемого месторождения, аналитические расчеты устойчивости элементов откосов. Результаты. Получены данные расчетов устойчивости откосов при отсутствии поверхностей ослабления в прибортовом массиве борта в висячем боку рудной залежи (висячий борт) и с учетом угла падения Главного разлома под углом 40–45° при формировании борта в лежачем боку (лежачий борт), а также с учетом сейсмичности района и уровня грунтовых вод. Определены параметры откосов бортов на предельном контуре. Полученные значения параметров правомерны при отсутствии в прибортовом массиве поверхностей ослабления. При наличии поверхностей ослабления с падением в выработку откосы необходимо заоткашивать строго по этим поверхностям.
ПУТИ ПОВЫШЕНИЯ ЭФФЕКТИВНОСТИ УСТАНОВОК ЭЛЕКТРОЦЕНТРОБЕЖНЫХ НАСОСОВ ДОБЫЧИ НЕФТИ
Authors: Копырин Владимир Анатольевич , Портнягин Алексей Леонидович , Хамитов Рустам Нуриманович
Published: November 2019

Актуальность работы обусловлена необходимостью верификации данных узла «погружной электродвигатель – внутрискважинный компенсатор», полученных на имитационной модели с результатами стендовых испытаний. Внутрискважинная компенсация реактивной мощности является относительно новой технологией повышения энергетической эффективности нефтедобывающих объектов по сравнению с существующими способами. Ввиду технических особенностей размещения внутрискважинных компенсаторов реактивной мощности в эксплуатационной колонне нефтедобывающей скважины, в некоторых случаях, экономически не целесообразно исследование установки электроцентробежного насоса с внутрискважинным компенсатором, с целью определения оптимальных параметров работы установки на реальном объекте. Поэтому разработка адекватной имитационной модели узла «погружной электродвигатель – внутрискважинный компенсатор» является важной задачей, позволяющей при минимальных материальных затратах решить проблемы построения, функционирования и модернизации установок электроцентробежных насосов. Цель: исследование внутрискважинного компенсатора реактивной мощности в программной среде Matlab Simulink и экспериментальные исследования погружного асинхронного электродвигателя с внутрискважинным компенсатором на лабораторном стенде. Объект: погружной асинхронный электродвигатель с внутрискважинным компенсатором реактивной мощности. Методы. При разработке имитационной модели погружного асинхронного электродвигателя использованы готовые блоки библиотеки SimPowerSystem программного комплекса MATLAB Simulink. Для проверки адекватности имитационной модели изготовлен погружной асинхронный электродвигатель и опытный образец внутрискважинного компенсатора реактивной мощности. Стендовые испытания проведены на заводе ООО «Алмаз», имеющем лицензированные лаборатории и аттестованных специалистов. Результаты. Разработана имитационная модель узла нагрузки «ПЭД-ВКРМ» для моделирования электромеханических процессов узла. Разработан опытный образец внутрискважинного компенсатора реактивной мощности. По результатам стендовых испытаний получены рабочие характеристики погружного асинхронного электродвигателя с внутрискважинным компенсатором. Проведена верификация данных, полученных на имитационной модели, с результатами эксперимента. Расхождения между расчетными и экспериментальными данными составили менее 10 %. Таким образом, разработанная имитационная модель узла нагрузки «ПЭД-ВКРМ» адекватно отображает электромеханические процессы узла. Определены перспективы проведения опытно-промышленных испытаний погружного асинхронного электродвигателя с внутрискважинным компенсатором реактивной мощности.
Vpliv suše na drobne korenine dreves in ektomikorizo v gozdnih ekosistemih
Authors: Mrak Tanja , Kraigher Hojka
Published: November 2019

Drought stress elicits many changes in tree fine roots and ectomycorrhizal fungi. Trees cope with drought through avoidance mechanisms or tolerance. Drought can result in changes in colonization by ectomycorrhizal fungi and in the structure of ectomycorrhizal communities. Survival of tree seedlings is supported through common mycelium networks. In moderate drought, there is greater colonization by ectomycorrhizal fungi compared to severe drought, resulting in several beneficial effects to the tree. Under drought, the frequency of ectomycorrhizal fungus Cenococcum geophilum Fr. often increases. C. geophilum sustains tree fine roots function and therefore roots are able to absorb water as soon as the drought period is over. Under drought, synthesis of recalcitrant organic compounds in roots, e.g. lignine, is increased. Recalcitrant compounds such as melanine are also found in C. geophilum, contributing to the accumulation of recalcitrant soil organic matter.
EFISIENSI DAN KINERJA PERBANKAN SYARIAH DI INDONESIA
Authors: Addury Multazam Mansyur
Published: November 2019

Penelitian ini bertujuan untuk menganalisis pengaruh dari efisiensi terhadap kinerja perbankan syariah dengan menggunakan pangsa pasar sebagai variabel moderator. Data yang digunakan pada penelitian ini berjumlah 7 Bank Umum Syariah yang berbentuk tahunan dari periode 2014 sampai 2018. Teknik analisis data yang digunakan yaitu analisis regresi data panel. Hasil penelitian membuktikan bahwa pertama, efisiensi perbankan syariah memiliki pengaruh yang signifikan terhadap kinerja perbankan syariah. Kedua, pangsa pasar bank syariah tidak berdampak signifikan dalam memoderasi hubungan antara efisiensi terhadap kinerja perbankan syariah.
Kata Kunci: Efisiensi, Kinerja, Pangsa Pasar, Bank Syariah
Dossiê Temático 2019 - Materiais para o ensino de matemática nos primeiros anos escolares: condensando saberes pedagógicos e disciplinares
Authors: Valente Wagner
Published: November 2019

Apresemtação do dossiê Materiais para o ensino de matemática nos primeiros anos escolares: condensando saberes pedagógicos e disciplinares
Penerapan Sistem Fuzzy Logic pada Alat Ukur Kadar Nutrisi pada Sistem Hidroponik
Authors: Azizah Andi , Waris Abdul , Sapsal Muhammad Tahir
Published: November 2019

Sistem hidroponik merupakan cara produksi tanaman yang sangat efektif. Pada umumnya pemberian nutrisi pada sistem ini tidak menyesuaikan banyak sedikitnya nutrisi yang dibutuhkan dari masa penanaman hingga waktu pemanenan. Tujuan penelitian ini adalah untuk menghasilkan alat ukur nutrisi berbasis fuzzy logic yang dapat digunakan pada sistem hidroponik. Pembuatan program fuzzy logic terdiri dari fuzzifikasi, aplikasi fungsi implikasi dan komposisi aturan, serta defuzzifikasi. Berdasarkan hasil penelitian yang telah dilakukan dapat diketahui bahwa sensor memenuhi kriteria yaitu tanggapan waktunya cepat, relatif stabil, pengaruh suhu yang relatif kecil, sensitif, linear dengan nilai R2= 0.996, kesalahan pembacaan yang relatif kecil yaitu 7.75%.
THE ANALYSIS ON MALE AND FEMALE TALK IN OPRAH WINFREY SHOW (SOCIOLINGUISTIC APPROACH)
Authors: Yuliananingrum Asih Tri , Suparno Suparno , Sujoko Sujoko
Published: November 2019

This article reports the result of research on male and female talk in Oprah Winfrey Show. The objectives of this research are to find out (1) what are the differences between males and females’ speech in Oprah Winfrey Show, (2) what are the similarity characteristics of educated people’s speech in public performance. The method used in this research was descriptive method using sociolinguistic approach. The research data were collected through downloading videos from website and watching them several times. The data were analyzed in four elements including grammar, intonation, turn taking system, and register. The result of the research shows (1) there are not many differences between males and females’ speech in Oprah Winfrey Show, (2) there are some characteristics of educated people’s speech in public performance. The findings of this study are expected to give some benefits in language study, especially English and English teaching. These differences styles of male and female in talk show give side-effect conversation in society.
A CORRELATIONAL STUDY BETWEEN EXTROVERSION PERSONALITY, VOCABULARY MASTERY, AND SPEAKING SKILL
Authors: Anjarani Shelia , Asib Abdul , Sulistyawati Hefy
Published: November 2019

The article is aimed at investigating the correlation between extroversion personality and vocabulary mastery toward speaking skill of the fourth semester students of English Education Department, Teacher Training and Education Faculty, Sebelas Maret University in the academic year of 2012/2013; both partially and simultaneously. The method of this study is correlational research. The population of the study is all of the fourth semester students while the sample is 30 students taken by simple random sampling technique. The technique used to analyze the data are simple, multiple correlation, and regression by using microsoft excel. The results of the study show that there is a positive correlation between extroversion personality and vocabulary mastery toward speaking skill both partially and simultaneously. The positive correlation indicates that extroversion personality and vocabulary mastery tend to go up or to go down together with speaking skill.
A CORRELATIONAL STUDY BETWEEN READING HABIT, VOCABULARY MASTERY, AND TRANSLATION ABILITY
Authors: Setyaningsih Widya , Sujoko Sujoko , Sarosa Teguh
Published: November 2019

This study aims finding out whether there is a correlation between (1) students’ reading habit and translation ability; (2) students’ vocabulary mastery and translation ability; and (3) students’ reading habit and vocabulary mastery toward translation ability. The study is conducted from May to June 2013 at English Education Department of Teacher Training and Education Faculty of Sebelas Maret University, and the population is all of sixth semester students. The sample is 34 students taken by cluster random sampling technique. The method used in this study is descriptive research method, with category of correlational study. The instruments in collecting the data are questionnaire and test. The techniques which are used to analyze the data are Simple Correlation and Multiple Regression Correlation. The result of the data analysis shows that in the level of significance a = 0.05 (1) there is a positive correlation between students’ reading habit and translation ability (r-./ = 0.448 > rt  = 0.339) and it is about 20.07%; (2) there is a positive correlation between students’ vocabulary mastery and translation ability (r-0/ = 0.467 > rt = 0.339) and it is about 21.81%; and (3) there is positive correlation between students’ reading habit, vocabulary mastery, and translation ability (R/.0 = 0.24 and Fo = 6.26 > Ft = 3.32) and it is about 28.76%.
Using Picture Series to Enhance Students’ Speaking Fluency
Authors: Agdhila Harta Diba , Sujoko Sujoko , Setyaningsih Endang
Published: November 2019

The objectives of the research are: (1) to find out whether or not picture series can improve students’ speaking fluency; (2) to describe the classroom situation when picture series is applied in teaching speaking. The subject of the research is the students of class X.8 of Senior High School. The research data were collected through observation, interview, field notes, document analysis, photographs, and tests including pre-test and post test. The qualitative data were analyzed by assembling the data, coding the data, comparing the data, building interpretation, and reporting the outcomes. Meanwhile, the quantitative data were analyzed by comparing the mean scores of the pre-test and the post test. The research findings show that Picture Series could improve: (1) students’ speaking fluency and (2) classroom situation of English class. The improvement of students’ speaking fluency can be seen from students’ mean score increased from 60.81 in pre-test into 73.78 in post-test 1 and it improves to 82.97 in post-test 2. The description of classroom situation includes :(a) The students looked very interested and enthusiastic to join the speaking class and (b) the students were more active during teaching learning process.
A Teacher’s Beliefs in Grammar and Her Teaching Practices: A Case Study of One Experienced EFL Teacher in A High School in Surakarta
Authors: Puspitasari Dani , Susilohadi Gunarso , Wahyuni Dewi Sri
Published: November 2019

Borg (2009) states that there is a relationship between people’s action and their belief and knowledge. It makes educational researchers take more concern with a similar phenomenon which occurs in teaching learning process. Borg claims that ‘teachers’ mental lives play a role in their instructional practices’. This qualitative research reports a case study of teacher’s beliefs in grammar and her grammar teaching practice in SMA Negeri 5 Surakarta. The data were collected by in-depth interview with the teacher, in-depth interview with students, and passive classroom observation. The data were analyzed by qualitative data analysis proposed by Miles and Huberman (1994). The result of the research indicates that the teacher’s beliefs of grammar and teaching grammar influence her grammar teaching practices.
Describing The Way of Teaching English to Students with Hearing Impairment
Authors: Pratama Aditya , Nurkamto Joko , Setyaningsih Endang
Published: November 2019

Despite of the numerous researches of English language teaching, there are not many researches about teaching English to students with hearing impairment, thus this research is implemented. The objectives of the research are to: (1) describe the implementation of teaching and learning English in XI grade students of SLB-B in Surakarta (2) identify the problems faced by teacher and students in the process of teaching and learning English, and (3) find out the students’ proficiency level in English. This research used qualitative naturalistic method. The data were collected through observation, interview, and document collecting. Coding was used in analyzing the data. The research finding shows that the students with hearing impairment are also able to understand English as long as the teacher uses various teaching method which are suitable with them. The students’ problems are coming from of their lack of language input which results in their imperfect pronunciation and limited vocabulary.
Cohesion in 20th Century American Short Stories Compiled By Jean A. McConochie, and Its Contribution to Teach Reading in Senior High School
Authors: Laksani Hening , Martono, Martono, , Setyaningsih Endang
Published: November 2019

Five types of cohesive devices are identified by Halliday and Hasan (1976), namely, reference, substitution, ellipsis, conjunction and lexical cohesion. Each of the five types is about the formal links within sentences. This qualitative research method describes the kind of cohesive devices employed in 20th Century American Short Stories and discusses its implication for developing teaching material for reading skill in Senior High School. The result of the study shows that cohesive devices mostly appeared in the short stories are lexical cohesion followed by references, conjunction, ellipsis and then substitution. The contribution of this research to English education is the cohesive devices can be used as a lesson material for teaching reading both as the example of language use in order to familiarize  about the use of pronouns, conjunctions and dictions, also it can be used to show the way to substitute or omit a word meaningfully and as the task students need to accomplish.

 
“Passport to The World 1” Based on Communicative Language Teaching Principle
Authors: Prihatiningsih Ayu Putri , Nurkamto Joko , Sarosa Teguh
Published: November 2019

The purposes underlying this study are (1) to describe an English textbook entitled “Passport to the World 1” in general; (2) to analyze the quality of the English textbook in developing communicative tasks and types of communicative materials. This research used a descriptive method. The data source of this research is the textbook for seventh grade of Junior High School, published in 2009 by PT Tiga Serangkai Pustaka Mandiri. In collecting data, this study takes four of ten units in the textbook randomly. The study uses theory of Cunningsworth (1995) to analyze the textbook in general and Communicative Language Teaching principles to analyze the tasks and material of the textbook. The result of the study shows that (1) the description of the textbook in general is fairly good (62.5 %); (2) the quality of communicative tasks is good (68.7%), and the quality of types of material in CLT is very good (75.03%). The textbook has all criteria of communicative tasks and materials, but each unit of the the textbook is still not complete. It means that the textbook is appropriate to be used and it  will be better if the teacher also completes each unit which is not appropriate communicative criteria by using other sources in order to develop students’ communicative competence.
Relationship Between Interest in Listening English Songs, Vocabulary Mastery Toward Listening Skill
Authors: Atmaja Dimas Aditya Dwi , Ngadiso Ngadiso , Setyaningsih Endang
Published: November 2019

Despite of numerous researches on listening, vocabulary, and interest, there are few researches that relate the three variables in one study. This research aims at finding the correlation between interest in listening to English songs, vocabulary mastery and listening skill. The study involves 29 students of a state school in Banyudono. They were selected as the sample by using cluster random sampling. The data on students’ interest in listening to English songs are collected by using questionnaire and data on students’ vocabulary mastery and listening skill are collected by using test. The techniques used to analyze the data are Simple and Multiple Linear Regression and Correlation. The results indicate that: (1) interest in listening to English songs contributes to listening skill; (2) similar interaction is found between vocabulary mastery and listening skill; and (3) interest in listening to English songs and vocabulary mastery simultaneously contribute to students’ listening skill.
Improving The Students’ Writing Skill by Using Picture
Authors: Kartika Arif , Nurkamto Joko , Pudjobroto A. Handoko
Published: November 2019

The objectives of this thesis are to describe whether picture improves students’ writing skill and to describe the classroom situation when picture is used in writing class. The method of the research was classroom action research.   The action research was conducted from January to April 2013 and was carried out in two cycles in 32 students of VIII A in SMPN 1 Jaten. The researcher collected the data using observation and questionnaire. After collecting the data, the researcher analyzed the data using qualitative and quantitative technique.  The research findings show that the using of picture could improve the students’ writing. It can be seen from the mean scores improvement of pre test, post test cycle 1 ,and post test cycle 2, were 51.62 for the pre-test, 68.00 for the post-test in cycle 1 and 78.31 for the post-test in cycle 2. The using of picture in teaching writing also could improve classroom situation in writing class.
Improving Students’ Speaking Skill Using Tgt (Teams-Game-Tournament)
Authors: Fajriya Nurul , Asib Abdul , Susilohadi Gunarso
Published: November 2019

This article is aimed at reporting the research findings of using TGT (Teams-Game-Tournament) to improve the students’ speaking skill. The subject of this research is the eighth grade of SMP N 1 Kartasura in the academic year of 2013/2014 which consisted of 32 students. The research method used is action research. Each cycle consists of four essential moments: planning, implementing the action, observing, and reflecting. The data are collected through observation, interview, field notes, questionnaire, and tests (pre-test ad post-test). The qualitative data were analyzed by reducing the data, displaying the data, and drawing conclusion/verifying. While the quantitative data were analyzed through descriptive statistics by calculating and then comparing the mean scores of pre-test and post-test. The research findings prove that using TGT can improve the students’ speaking skill. The students’ mean score increases from pre-test of 4.4 to post-test 1 of 5.9, and post-test 2 of 6.8. Using TGT can improve the effectiveness of speaking activity in teaching and learning process as well. The students got more opportunities to practice speaking because the speaking activities were not dominated by the talkative students only.
The Correlation of Students’ Interest and Vocabulary Mastery Toward Writing Skill
Authors: Wulansari Fitria , Asrori Muh. , Wahyuni Dewi Sri
Published: November 2019

This research aimed to find out the correlation of: (1) students’ interest in learning to writing skill; (2) vocabulary mastery to writing skill; and (3) students’ interest in learning and vocabulary mastery simultaneously to writing skill. This study used a correlational method with a cluster random sample of 31 students of the eleventh grade students of SMK Batik 2 Surakarta in the academic year 2013/2014. The researcher used questionnaire, objective tests and essay test as the instrument to collect the data. Single and Multiple Linear Regression and Correlation were used to analyze the data.  The research findings show that: (1) students’ interest in learning brings 39.69% contribution to writing skill; (2) vocabulary mastery brings 40% contribution to writing skill; and (3) students’ interest in learning and vocabulary mastery simultaneously bring 47.98% contribution to writing skill.
The Profile of Effective Readers and Their Learning Achievement
Authors: Oktavia Riana , Nurkamto Joko , Kristiandi Kristiandi
Published: November 2019

This article discusses the characteristics of effective readers in reading course books and their learning achievement. It is based on a research at English Education Department of FKIP UNS Surakarta 2013/ 2014. The research used qualitative approach in term of case study that deeply describes the case. It employed purposive sampling. The data were collected through observations, interviews, and documents analysis. The effective readers are influenced by their family, friends, lecturers, and their motivation. They read for getting something new and as background knowledge in learning process. They usually read alone, plan the schedule and prefer to read English books. Reading strategies of the effective readers are text books marking to the key points, making notes, keep reading, find easy readings, focus and understand by the context or read multiple sources. They are aware that appropriate reading give positive effects to their learning achievement. So, they always use their own reading strategies and it makes their better achievement.
The Use of Round Table Technique to Improve Students’ Writing Skill
Authors: Putri Neta Elisabeth , Susilohadi Gunarso , Sulistyawati Hefy
Published: November 2019

The objective of this article is to report a study on how Round Table Technique is implemented effectively so that it can improve students’ writing skill. This study was an action research. The subject of the research was class X-7 students of SMA Negeri Kebakkramat in the academic year 2013/2014. The data were collected through observation, interview, diary, and test. The findings of the research show that when Round Table technique was used effectively, the teaching and learning process was described as: active learning, learner – centered, and the activities were taking turns to jot down phrases and group discussion. In order to implement Round Table technique effectively, there were some points to ponder to suit the students’ condition and need as follows: (1) determining students’ group, (2) classroom management, and (3) the adaptation to Round Table technique.
Item Analysis of Preparation Test for English National Examination
Authors: Laela Madiana , Rochsantiningsih Dewi , Martono Martono
Published: November 2019

This research aims to reveal the quality of English national examination preparation test in terms of qualitative and quantitative aspects. Qualitative aspect includes content validity, technical item quality and cognitive domain learning outcome while quantitative aspect include reliability, difficulty level, item discrimination, and distractor effectiveness. Sample was taken from 3 out of 10 schools in Pati district using simple random sampling. This research employs both qualitative and quantitative analysis in which expert judgement is used to analyze content validity and technical item quality while ITEMAN is used for quantitative analysis. The result showed that the test has good content validity, 99.06% items appropriate with competence being measured, good technical item quality and most items (81.13%)are categorized as cognitive domain learning outcome C2 (Understand). Moreover, the test has high reliability index (> 0.8), fair difficulty, and good discrimination. However, 35.85% items have ineffective distractors.
Correlation Between Cohesive Devices Mastery and Learning Motivation Toward Reading Comprehension
Authors: Utomo Arining Tyas , Asrori Muh. , Sulistyawati Hefy
Published: November 2019

The aims of the research are to investigate whether there is a positive correlation between (1) cohesive devices mastery and reading comprehension; (2) learning motivation and reading comprehension; (3) cohesive devices mastery, learning motivation simultaneously toward reading comprehension. The method used in this research is correlation. The study was conducted at a senior high school in Sukoharjo. The population of the research consists of 253 students. The sample is 34 students taken by cluster random sampling. The instruments in collecting the data are questionnaire and objective test. The technique of analyzing the data are simple and multiple linear regression and correlation. The result of the research shows that there is a positive correlation between (1) cohesive devices mastery and reading comprehension; (2) learning motivation and reading comprehension; (3) cohesive devices mastery, learning motivation simultaneously toward reading comprehension. Based on the research result, it can be concluded that cohesive devices mastery and learning motivation give contribution in the improvement of reading comprehension.
A Study On Frustration in Fantine on Les Miserables Novel By Victor Hugo: Psychological Approach and Its Implementation
Authors: Titaranti Amrita , Siswantoro Siswantoro , Rochsantiningsih Dewi
Published: November 2019

Novel has its own richness which is worth exploring. It has many kinds of subject discussions. The subject of frustration is chosen based on Fantine, the most influential woman character in this novel. From her life, frustration as a psychological effect appears. The discussion is not only in literary discussion, but it is also used as teaching material as the tool to complete the need of literary teaching among senior high school students. The data consist of every sentence or conversation which reveal Fantine’s thought, feeling, or behavior. They are collected by reading the novel empirically and analyzed by using flow model by Miles and Huberman (1994). The findings of the study show that (a) Fantine’s evidences of frustration are anxiety, anger and aggression, apathy and depression, cognitive trouble, health disruption, and physical appearance. (b) Fantine’s causes of frustration are unpredictable incident, uncontrolled incident, incident which challenges human limitation, and internal conflict. (c) Fantine’s defense mechanisms are suppression, formed reaction, displacement, and denial. (d) And then summarize of Fantine’s story is used as teaching reading material by putting it in lesson plan which uses curriculum 2013.