bluuebunny/crossref_metadata_2025 huggingface dataset:
Effective Use of Plant Proteins for the Development of “New” Foods
Authors: Yano Hiroyuki , Fu Wei
Published: April 2022

Diversity in our diet mirrors modern society. Affluent lifestyles and extended longevity have caused the prevalence of diabetes and sarcopenia, which has led to the increased demand of low-carb, high-protein foods. Expansion of the global population and Westernization of Asian diets have surged the number of meat eaters, which has eventually disrupted the supply–demand balance of meat. In contrast, some people do not eat meat for religious reasons or due to veganism. With these multiple circumstances, our society has begun to resort to obtaining protein from plant sources rather than animal origins. This “protein shift” urges food researchers to develop high-quality foods based on plant proteins. Meanwhile, patients with food allergies, especially gluten-related ones, are reported to be increasing. Additionally, growing popularity of the gluten-free diet demands development of foods without using ingredients of wheat origin. Besides, consumers prefer “clean-label” products in which products are expected to contain fewer artificial compounds. These diversified demands on foods have spurred the development of “new” foods in view of food-processing technologies as well as selection of the primary ingredients. In this short review, examples of foodstuffs that have achieved tremendous recent progress are introduced: effective use of plant protein realized low-carb, high protein, gluten-free bread/pasta. Basic manufacturing principles of plant-based vegan cheese have also been established. We will also discuss on the strategy of effective development of new foods in view of the better communication with consumers as well as efficient use of plant proteins.
Onicescu’s Informational Energy and Correlation Coefficient in Exponential Families
Authors: Nielsen Frank
Published: April 2022

The informational energy of Onicescu is a positive quantity that measures the amount of uncertainty of a random variable. However, contrary to Shannon’s entropy, the informational energy is strictly convex and increases when randomness decreases. We report a closed-form formula for Onicescu’s informational energy and its associated correlation coefficient when the probability distributions belong to an exponential family. We show how to instantiate the generic formula for several common exponential families. Finally, we discuss the characterization of valid thermodynamic process trajectories on a statistical manifold by enforcing that the entropy and the informational energy shall vary in opposite directions.
Identification and Validation of Reliable Reference Genes for Gene Expression Studies in Koelreuteria paniculata
Authors: Gao Kai , Khan Wasif Ullah , Li Juan , Huang Sai , Yang Xiong , Guo Ting , Guo Bin , Wu Ruqian , An Xinmin
Published: April 2022

RT-qPCR is considered a rapid and reliable technique for analyzing gene expression. This technique is commonly used to analyze the expression of various genes at diverse transcriptional levels in different samples. However, few studies have characterized ornamental Koelreuteria species for reliable reference genes. In this study, eight reference genes were evaluated as controls in RT-qPCR with SYBR green to quantify gene expression in different Koelreuteria paniculata samples. All selected reference genes showed a broad range of Ct values in all samples, which was supportive of their variable expression. Our results showed significant variation in the stable expression of K. paniculata genes. Sample data, analyzed using geNorm, NormFinder, and BestKeeper, showed that phospholipase (PLA2) and β-actin (ACT) were the most suitable and statistically reliable reference genes, whereas ribosomal protein L13 (RPL13) and elongation factor 1-α (EF1α) were less stable and unsuitable for use as internal controls. To compare gene expression levels, two or more reference genes should be used for data normalization. Thus, the stability and expression of both PLA2 and ACT were believed to provide better normalization and quantification of the transcript levels for gene expression studies in K. paniculata.
Influence of Scuba Diving on the Quality of Life of People with Physical Disabilities
Authors: Henrykowska Gabriela , Soin Joanna , Pleskacz Katarzyna , Siermontowski Piotr
Published: April 2022

The aim of the study was to assess quality of life related to mental and physical health among divers and non-divers with physical disabilities. The examined group consisted of 240 disabled people (both genders). The SF-36 questionnaire (Short-Form Health Survey) was used to measure the overall sense of health-related quality of life. Moreover, the authors’ survey was also used in the study. There was a significant difference (p < 0.05) in the self-assessment of the quality of life (physical functioning, social functioning, mental health, and vitality) between the examined diving and non-diving groups. In other areas evaluated with the use of the SF-36 questionnaire, i.e., limitation in performing roles due to emotional problems and pain, limitations in performing roles due to physical health, a tendency to a higher rating was noticed in the group of divers. Scuba diving can improve various components of the life-quality of people with disabilities, and in general can be seen as a form of physical activity and rehabilitation for people with disabilities. However, it is necessary to conduct extensive research in this area.
Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
Authors: Palomo-López Nora , Rodríguez-Rodríguez Ana , Martín-Villén Luis , Mendoza-Prieto María , Ruiz de Azúa-López Zaida , Sempere-Bordes Lluis , Boyero-Corral Laura , Daga-Ruiz Domingo , Gordillo-Brenes Antonio , Pacheco-Sánchez María , Perez-Villares José Miguel , Vilches-Arenas Ángel , Egea-Guerrero Juan José
Published: April 2022

The objective was to quantify oxidative stress resulting from ischemia during the donation process, using malondialdehyde (MDA) measurement, and its modulation by the administration of melatonin. We designed a triple-blind clinical trial with donors randomized to melatonin or placebo. We collected donors by donation after brain death (DBD) and controlled donation after circulatory death (DCD), the latter maintained by normothermic regional perfusion (NRP). Melatonin or placebo was administered prior to donation or following limitation of therapeutic effort (LTE). Demographic variables and medical history were collected. We also collected serial measurements of MDA, at 60 and 90 min after melatonin or placebo administration. A total of 53 donors were included (32 from DBD and 21 from DCD). In the DBD group, 17 donors received melatonin, and 15 placebo. Eight DCD donors were randomized to melatonin and 13 to placebo. Medical history and cause for LTE were similar between groups. Although MDA values did not differ in the DBD group, statistical differences were observed in DCD donors during the 0–60 min interval: −4.296 (−6.752; −2.336) in the melatonin group and −1.612 (−2.886; −0.7445) in controls. Given the antioxidant effect of melatonin, its use could reduce the production of oxidative stress in controlled DCD.
Extramedullary Acute Leukemia—Still an Unforeseen Presentation
Authors: Rochate Dina , Pavão Carolina , Amaral Rui , Viveiros Carolina , Cabeçadas José , Carneiro Vitor , Fraga Cristina
Published: April 2022

Myeloid sarcomas (MS) are rare extramedullary (EM) hematological tumors that generally arise during the natural course of acute myeloid leukemia (AML), occurring concomitantly with the onset of systemic leukemia; it can also occur following onset but rarely before. Common sites of EM involvement include the lymph nodes, skin, soft tissue, bone and peritoneum. Herein, we report the case of a 63-year-old man who presented EM AML upon initial diagnosis involving the bone marrow, lymph nodes and skin (leukemia cutis). A diagnosis was made based on immunohistochemistry (IHC). This case presents a diagnostic dilemma due to its atypical presentation and the sites involved. It also highlights the importance of IHC in the diagnosis of EM AML. The potential role of hypomethylating agents and Venetoclax in cases not eligible for hematopoietic stem cell transplant are also discussed.
Regulation of BcMYB44 on Anthocyanin Synthesis and Drought Tolerance in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis Makino)
Authors: Hao Yuli , Wang Jianjun , Hu Chunmei , Zhou Qian , Mubeen Hafiz Muhammad , Hou Xilin
Published: April 2022

The purpose of this study was to explore the regulation of BcMYB44 on anthocyanin synthesis and drought tolerance of non-heading Chinese cabbage. The BcMYB44 gene was cloned from the purple inbred line ‘NJZX1-3’ and its green mutant ‘NJZX1-0’. Sequence analysis confirmed that BcMYB44 belongs to the R2R3-MYB family and has the highest homology with BnMYB44. Subcellular localization revealed that BcMYB44 is a nuclear protein. Yeast two-hybrid (Y2H) and Bimolecular Fluorescent Complimentary (BiFC) experiments showed that BcMYB44 interacts with BcPAP1 and BcEGL3. Pigment detection of BcPAP1 and BcMYB44 protein activity in N. benthamiana indicates that BcMYB44 plays a negative regulatory role by inhibiting the expression of key structural genes (F3H, DFR, etc.) in anthocyanin synthesis. Virus-induced gene silencing (VIGS) further confirmed this inhibition. Analysis of drought tolerance of non-heading Chinese cabbage based on VIGS showed that pTY-S plants are more resistant to drought than pTY-BcMYB44 plants. The results indicate that BcMYB44 has a positive regulatory role in drought stress, which most likely is achieved by inhibiting anthocyanin accumulation, regulating stomatal movement, and improving osmotic regulation and homeostasis of reactive oxygen species (ROS).
Sleep Quality, Insomnia, Anxiety, Fatigue, Stress, Memory and Active Coping during the COVID-19 Pandemic
Authors: Alqahtani Jaber S. , AlRabeeah Saad M. , Aldhahir Abdulelah M. , Siraj Rayan , Aldabayan Yousef S. , Alghamdi Saeed M. , Alqahtani Abdullah S. , Alsaif Sulaiman S. , Naser Abdallah Y. , Alwafi Hassan
Published: April 2022

Background: The current study aimed to evaluate the impact of the coronavirus (COVID-19) pandemic on sleep quality, insomnia, anxiety, stress, fatigue and active coping in the United States. Methods: This was a cross-sectional study using a publicly available database taken from the Boston College COVID-19 Sleep and Well-Being Dataset. We have selected the most recent data that included information about sleep quality and other measures, including insomnia, anxiety, stress, fatigue and coping, collected between 22 February–8 March 2021. Results: A total of 476 subjects were included in the analysis. The mean (SD) age of the study population was 38.8 (17.8) years, and there were more females (85%) than males. The population had a mean (SD) score of the Pittsburgh Sleep Quality Index (PSQI) of 6 (3.2), with 65% having the prevalence of poor sleep quality (defined as PSQ ≥ 5; n = 311). The mean (SD) score for Insomnia Severity Index (ISI) was 6.9 (5.2), with 55 subjects (11.5%) having clinical insomnia (defined as ISI ≥ 15); of whom 9% had severe clinical insomnia. There were positive correlations between PSQI and ISI (r = 0.76, p < 0.001), PROMIS fatigue scale (r = 0.53, p < 0.001), Generalized Anxiety Disorder-7 (GAD-7) (r = 0.46, p < 0.001), and Perceived Stress Scale (PSS) (r = 0.44, p < 0.001). The PSQI was inversely correlated with the John Henryism Active Coping Scale (JHACS) and memory scale. In the multivariate regression model, JHACS, ISI, fatigue, PSS and GAD-7 were significant predictors of PSQI, and these variables accounted for 62% of the variance of PSQI, adjusted for age and gender. Conclusion: An important contribution to the literature is made by this research, which demonstrates the significant prevalence of poor sleep quality and its association with insomnia and other mental and physical well-being. It also underlines the need to prioritise policy and public health efforts to address sleep issues that have substantial health and economic effects for both individuals and the population at large.
Risk of Hypertension and Type 2 Diabetes in Relation to Changes in Alcohol Consumption: A Nationwide Cohort Study
Authors: Choi Jae Woo , Han Euna , Kim Tae Hyun
Published: April 2022

Little is known about the risk of hypertension and type 2 diabetes in relation to changes in frequency and amount of alcohol consumption. This study investigated associations between changes in alcohol consumption and the risk of both conditions. This study included 96,129 individuals without hypertension and/or diabetes mellitus aged ≥ 20 years between 2006 and 2008, with follow-up until 31 December 2015. This study identified 29,043 and 18,784 incident cases of hypertension and type 2 diabetes, respectively, during an average follow-up period of 6.2 ± 2.6 and 6.9 ± 1.9 years. This study measured changes in frequency and amount of alcohol consumption using standardized self-administered questionnaires over approximately 2 years. Hazard ratio (HR) and 95% confidence interval (CI) were calculated for the respective risks of the two conditions. Repeated occasional or frequent binge drinking was associated with an increased risk of hypertension (HR: 1.16 or 1.32; 95% CI: 1.11, 1.21 or 1.16, 1.51) and type 2 diabetes (HR: 1.14 or 1.36; 95% CI: 1.09, 1.20 or 1.17, 1.58) compared with continuous nondrinking. Reductions as well as increases in frequency of alcohol consumption among binge drinkers were associated with higher hypertension (HR: 1.29 or 1.30; 95% CI: 1.11, 1.49 or 1.13, 1.49) and type 2 diabetes (HR: 1.26 or 1.56; 95% CI: 1.06, 1.49 or 1.34, 1.81) risk. This study demonstrated that repeated binge drinking, even with a reduction of weekly alcohol consumption frequency, was associated with a higher risk of hypertension and type 2 diabetes.
Risk of Burnout among Emergency Department Staff as a Result of Violence and Aggression from Patients and Their Relatives
Authors: Schablon Anja , Kersten Jan Felix , Nienhaus Albert , Kottkamp Hans Werner , Schnieder Wilfried , Ullrich Greta , Schäfer Karin , Ritzenhöfer Lisa , Peters Claudia , Wirth Tanja
Published: April 2022

Emergency department staff are often affected by incidents of violence. The aim of the study was to generate data on the frequency of violence by patients and accompanying relatives and the correlation between experienced aggression, a possible risk of burnout and a high sense of stress. Additionally, the buffering effect of good preventive preparation of care staff by the facility on aggressive visitors and patients was examined. In this cross-sectional study, members of the German Society for Interdisciplinary Emergency and Acute Medicine were surveyed. The investigation of risk factors, particularly experiences of verbal and physical violence, as well as exhaustion and stress, was carried out using ordinal regression models. A total of 349 staff from German emergency departments took part in the survey, 87% of whom had experienced physical violence by patients and 64% by relatives. 97% had been confronted with verbal violence by patients and 94% by relatives. Violence by relatives had a negative effect on perceived stress. High resilience or effective preparation of employees for potential attacks was shown to have a protective effect with regard to the burnout risk and perceived stress. Therefore, management staff play a major role in preventing violence and its impact on employees.
Exploring Risk Factors of Recall-Associated Foodborne Disease Outbreaks in the United States, 2009–2019
Authors: Sanchez Emily , Simpson Ryan , Zhang Yutong , Sallade Lauren , Naumova Elena
Published: April 2022

Earlier identification and removal of contaminated food products is crucial in reducing economic burdens of foodborne outbreaks. Recalls are a safety measure that is deployed to prevent foodborne illnesses. However, few studies have examined temporal trends in recalls or compared risk factors between non-recall and recall outbreaks in the United States, due to disparate and often incomplete surveillance records in publicly reported data. We demonstrated the usability of the electronic Foodborne Outbreak Reporting System (eFORS) and National Outbreak Reporting System (NORS) for describing temporal trends and outbreak risk factors of food recalls in 1998–2019. We examined monthly trends between surveillance systems by using segmented time-series analyses. We compared the risk factors (e.g., multistate outbreak, contamination supply chain stage, pathogen etiology, and food products) of recalls and non-recalls by using logistic regression models. Out of 22,972 outbreaks, 305 (1.3%) resulted in recalls and 9378 (41%) had missing recall information. However, outbreaks with missing recall information decreased at an accelerating rate of ~25%/month in 2004–2009 and at a decelerating rate of ~13%/month after the transition from eFORS to NORS in 2009–2019. Irrespective of the contaminant etiology, multistate outbreaks according to the residence of ill persons had odds 11.00–13.50 times (7.00, 21.60) that of single-state outbreaks resulting in a recall (p < 0.001) when controlling for all risk factors. Electronic reporting has improved the availability of food recall data, yet retrospective investigations of historical records are needed. The investigation of recalls enhances public health professionals’ understanding of their annual financial burden and improves outbreak prediction analytics to reduce the likelihood and severity of recalls.
Understanding the Interaction between Regulatory Focus and Message Framing in Determining Chinese Consumers’ Attitudes toward Artificial Meat
Authors: Shi Hongxu , Ma Peihua , Zeng Yinchu , Sheng Jiping
Published: April 2022

While production and consumption of meat cast a shadow over the prospects for sustainable development, artificial meat may be the solution. However, consumer acceptability of artificial meat is a major impediment to its use as a suitable alternative. This study analyzed the relationship between regulatory focus and consumer acceptance of artificial meat using randomized controlled trial data. Results showed that promotion focus results in a higher acceptance of artificial meat products due to a higher perceived benefit and lower perceived risk, whereas prevention focus results in a lower acceptance of artificial meat products due to perceived benefit being lower and perceived risk being higher. The moderating effect of the message framing was investigated employing structural equation modeling (SEM). It was discovered that a gain-oriented message framing could greatly strengthen the association between promotion focus and perceived benefit, whereas an avoidance-oriented message framing could significantly diminish the relationship between prevention focus and perceived risk. This study has crucial implications for how policymakers and industries communicate with consumers about artificial meat.
Influence of Spirituality and Religiosity of Cancer Patients on Their Quality of Life
Authors: Majda Anna , Szul Natalia , Kołodziej Kinga , Wojcieszek Agata , Pucko Zygmunt , Bakun Kinga
Published: April 2022

Taking into account the spiritual and religious dimensions is important when it comes to taking care of patients with cancer and their quality of life. The study aimed to show the degree of dependence between the level of spirituality/religiosity of people who have just been diagnosed with cancer or have been diagnosed with cancer in the past and their quality of life. The Daily Spiritual Experience Scale (DSES), the EORTC QLQ-C30 quality of life and EORTC QLQ-FA12 fatigue-related quality of life questionnaire were used. One hundred one respondents of the Catholic faith obtained 65.22 points in DSES; 49.84 points on the QLQ-C30 functioning scale, 58.75 points on the physical scale, 60.73 points on the social scale, 50.17 points on the emotional scale, 64.69 points on the cognitive scale, 55.45 points in fulfilling one’s role and 28.38 points in financial impact. In the QLQ-FA12, respondents obtained 45.94 points on the physical scale, 47.53 points on the emotional scale and 30.69 points on the cognitive scale. In the respondents’ opinion, fatigue was the disease that reduced their quality of life the most—on average, 51.27 points. The oncological patients were characterized by a high level of spirituality/religiosity and an average level of quality of life. Spirituality/religiosity had a positive relationship with physical, emotional and social functioning. On the other hand, it was negatively associated with disease symptoms, such as pain or emotional and physical fatigue. Future research is needed in the context of the quality of life, focused on the spiritual and religious sphere of functioning of cancer patients, conducted in various cultural, ethnic and religious circles, which can serve to improve the education of nurses and develop their spiritual competences.
The Role of Self-Efficacy, Work-Related Autonomy and Work-Family Conflict on Employee’s Stress Level during Home-Based Remote Work in Germany
Authors: Lange Martin , Kayser Ina
Published: April 2022

Home-based remote work becomes increasingly popular. The facets of remote work, especially working from home, are multifaceted and can become stressors that affect a person’s health. At the same time, self-efficacy is an important personal resource to deal with health-related stressors. The objective of this study is therefore to explore the relationship between self-efficacy (SE), work-related stress (WRS), health outcomes (health and anxiety), contributing factors (autonomy and experience) and work-family conflict (WFC) in a remote work setting. Using a PLS-model (partial least square) with a sample of n = 5163 responses, we found that SE significantly reduces WRS (β = −0.164; p < 0.001). Moreover, WFC increases WRS and anxiety, while SE reduces WFC and mediates health outcomes (anxiety: β = −0.065; p < 0.001; health: β = −0.048; p < 0.001). At the same time, autonomy (β = 0.260; p < 0.001) and experience (β = 0.215; p < 0.001) increase SE. Our results have high practical implications for employers and employees, underlining the importance of self-efficacy as a personal resource to buffer WRS and WFC while promoting overall health at the same time.
The Effect of Sleep–Wake Routines on the Negative Emotional States and Aggressive Behaviors in Adults with Autism Spectrum Disorders (ASD) during the COVID-19 Outbreak
Authors: Levante Annalisa , Petrocchi Serena , Colombi Costanza , Keller Roberto , Narzisi Antonio , Masi Gabriele , Lecciso Flavia
Published: April 2022

Disruption in routine may be related to experiencing negative emotional states and to aggressive behaviors in individuals with Autism Spectrum Disorder (ASD). The lockdown because of COVID-19 contributed to the disruption of individuals’ routines, including the sleep–wake cycle. The current study tested a relationship between the adherence to the sleep–wake routine and aggressive behaviors via the mediation role of negative emotional states (i.e., anxiety and anger). Forty-three parents of adults with ASD completed a web-based questionnaire about their life condition during the first lockdown (April–May 2020). Preliminary analyses showed a worsening in the adults’ aggressive behaviors during the lockdown in comparison to before it (Z = −3.130; p = 0.002). In the mediation models, the relationship between the adherence to the sleep–wake routines and aggressive behaviors was significant. The models showed the hypothesized mediated relationships among the adherence to the sleep–wake routines, negative emotional states, and aggressive behaviors (Model 1: F (1, 41) = 10.478, p < 0.001; Model 2: F(1, 41) = 9.826, p = 0.003). The findings confirmed the potential protective role of the adherence to the sleep–wake routines for the emotional and behavioral adjustment of adults with autism. Theoretical and practical contributions of the study were discussed; indeed, our results may inform parent-coaching as well as intervention programs for individuals with ASD given that adequate sleep hygiene may contribute to improvements in internalizing/externalizing behaviors.
Changes in the Dissolved Organic Matter Characteristics Released from Sediment According to Precipitation in the Namhan River with Weirs: A Laboratory Experiment
Authors: Oh Haeseong , Choi Jung-Hyun
Published: April 2022

In this study, changes in the properties of dissolved organic matter (DOM) released from sediments into water layers were investigated. To analyze the spatial and temporal variation in dissolved organic carbon (DOC), sediment and bottom water samples were collected upstream of the Gangcheon, Yeoju, and Ipo weirs of the Namhan River during the rainy and non-rainy seasons. The initial DOC was correlated with precipitation (R2 = 0.295, p = 0.034) and residence time (R2 = 0.275, p = 0.040). The change in the bottom water DOC concentration resulted from the DOC released from the sediments, which may cause water quality issues in the bottom water. The fluorescence analysis revealed that the DOM contained higher levels of hydrophilic and low-molecular-weight (LMW) organic matter in the non-rainy season and higher levels of hydrophobic and high-molecular-weight (HMW) organic matter in the rainy season. Since the Namhan River is the main resource of drinking water for the Seoul metropolitan area, our results can help to optimize the drinking water treatment process by reflecting the DOM characteristics that vary with the seasons. Furthermore, the statistical analysis confirmed that the nutrient content of pore-water and sediment can be used to estimate the DOM release rate from the sediment to the water layer. The results of this study provide a better understanding of DOM movement in aquatic ecosystems and the influences of rainfall on the water quality of the surface waterbody.
Health Statistics in Australia: What We Know and Do Not Know
Authors: Madden Richard , Fortune Nicola , Gordon Julie
Published: April 2022

Australia is a federation of six states and two territories (the States). These eight governmental entities share responsibility for health and health services with the Australian Government. Mortality statistics, including causes of death, have been collected since the late 19th century, with national data produced by the (now) Australian Bureau of Statistics (ABS) from 1907. Each State introduced hospital in-patient statistics, assisted by State offices of the ABS. Beginning in the 1970s, the ABS conducts regular health surveys, including specific collections on Aboriginal and Torres Strait Islander peoples. Overall, Australia now has a comprehensive array of health statistics, published regularly without political or commercial interference. Privacy and confidentiality are guaranteed by legislation. Data linkage has grown and become widespread. However, there are gaps, as papers in this issue demonstrate. Most notably, data on primary care patients and encounters reveal stark gaps. This paper accompanies a range of papers from expert authors across the health statistics spectrum in Australia. It is hoped that the collection of papers will inform interested readers and stand as a comprehensive review of the strengths and weaknesses of Australian health statistics in the early 2020s.
Views and Attitudes of Blood Donors toward Blood Donation during the COVID-19 Pandemic in Thrace Region, Greece
Authors: Gkirtsou Christina , Konstantinidis Theocharis , Cassimos Dimitrios , Konstantinidou Eleni I. , Kontekaki Eftychia G. , Rekari Viki , Bezirtzoglou Eugenia , Martinis Georges , Stergiannis Pantelis ,   for Thrace Study Group on Blood Donors’ Attitude during the COVID-19 Pandemic
Published: April 2022

The COVID-19 pandemic has been going on for the last two years and it has affected our society and, amongst other things, has had a negative impact on blood donation, which has led to a significant reduction in blood supplies worldwide. The imposed restrictions in terms of physical presence and transportation, and the fear of the unknown, have aggravated the situation. In Greece, after the first cases of COVID-19 were reported, the blood supplies at the blood transfusion units (BTUs) were dramatically reduced. Although the blood transfusions were lessened during the COVID-19 pandemic period, the blood stocks at all the BTUs of the country were also reduced.
Using Co-Design to Develop a Health Literacy Intervention with Socially Disadvantaged Adolescents
Authors: Goss Hannah R. , Smith Craig , Hickey Laura , Issartel Johann , Morrissey Janis , Murrin Celine , Spillane Ailbhe , Belton Sarahjane
Published: April 2022

The aim of this study was to initiate a co-design process with adolescents to inform the development of a targeted health literacy intervention for implementation in designated socioeconomically disadvantaged post-primary schools in Ireland. Purposely developed vignettes were explored in a series of eight workshops that were conducted separately with staff (n = 26) and students (n = 33) across four schools. Data was analysed using content analysis. A number of key health topics were identified as important and influential for the participants in this context: food choices, mental health and wellbeing, physical activity and sedentary behaviour, sleep and substance misuse. Participants also suggested many health-related capacity building actions. Participants recognized that many of these health topics and capacity building actions were intertwined and also highlighted that some of these actions may be more feasible and/or impactful than others. For example, students and school staff both indicated the need to use relevant, applied and engaging approaches to improve health literacy and subsequent health behaviour. The co-design process adopted empowered stakeholders to actively engage in the design and development of future intervention strategies, which may increase the likelihood of acceptability, effectiveness and sustainability of the resulting intervention.
Effect of Farmland Scale on Farmers’ Application Behavior with Organic Fertilizer
Authors: Chen Yushi , Fu Xinhong , Liu Yuying
Published: April 2022

The application of organic fertilizer is an important measure for improving the quality of agricultural products and realizing the sustainable development of agriculture. The original small-scale family business model in China is no longer suitable for the development of modern agriculture. Large-scale agricultural business has become the mainstream trend, accompanied by the increasingly active land-transfer market. It is, therefore, necessary to determine whether farmland scale influences farmers’ organic fertilizer applications in China. Based on the analysis of the influence mechanism of farmland scale on farmers’ organic fertilizer application behaviors, empirical tests were conducted using survey data from 812 citrus farmers in Sichuan Province, China. The results show that the large-scale farmers are more likely to apply organic fertilizer and increase the application intensity than small-scale farmers and that the heterogeneity of farmers also affect their organic fertilizer application behavior. The results suggest that the government should encourage farmers to conduct moderate-scale management and promote their application behavior with organic fertilizer by improving awareness, strengthening education and training, and providing financial support.
The Effect of the Swimmer’s Trunk Oscillation on Dolphin Kick Performance Using a Computational Method with Multi-Body Motion: A Case Study
Authors: Chen Zhiya , Li Tianzeng , Yang Jin , Zuo Chuan
Published: April 2022

The effect of a specific Chinese swimmer’s trunk oscillation on dolphin kick was investigated in order to optimize competitive swimming movement. Using a numerical simulation method based on multi-body motion, different swimmer’s trunk oscillation during a dolphin kick was analyzed. The simulation was conducted using 3D incompressible Navier–Stokes equations and renormalization group k-ε turbulence model, combined with the Volume of Fluid method to capture the water surface. The simulation’s results were evaluated by comparing them with experimental data and with previous studies. The net streamwise forces, mean swimming velocity, and joint moments were also investigated. There was a positive correlation between the mean swimming velocity and the amplitudes of the swimmer’s trunk oscillation, where the Pearson correlation coefficient was 0.986 and the selected model was statistically significant (p < 0.05). In addition, as the mean swimming velocity increased from 1.42 m/s in Variant 1 to 2 m/s in Variant 5, the maximum positive moments of joints increased by about 24.7% for the ankles, 27.4% for the knees, −3.9% for the hips, and 5.8% for the upper waist, whereas the maximum negative moments of joints increased by about 64.5% for the ankles, 28.1% for the knees, 23.1% for the hips, and 10.1% for the upper waist. The relationship between the trunk oscillation and the vortices was also investigated. Therefore, it is recommended that swimmers should try to increase the amplitudes of trunk oscillation to increase their swimming velocity. In order to achieve this goal, swimmers should increase strength training for the ankles, knees, and upper waist during the upkick. Moreover, extra strength training is warranted for the ankles, knees, hips, and upper waist during the downkick.
Academic Teachers about Their Productivity and a Sense of Well-Being in the Current COVID-19 Epidemic
Authors: Bartkowiak Grażyna , Krugiełka Agnieszka , Dama Sebastian , Kostrzewa-Demczuk Paulina , Gaweł-Luty Elżbieta
Published: April 2022

This article looked at the situation of university teachers in Poland during the COVID-19 epidemic as a result of their need to work remotely. The study was conducted in the first stage (I) on 21 academicians and in the second stage (II) on 18 academicians. The study was conducted to determine the level of productivity of the study group in their online learning competencies in relation to their well-being, as well as during the height, and weakening of the epidemic. The results of the survey conducted, especially during the height of the epidemic, indicated varying levels of self-evaluation of their productivity in relation to their digital competencies linked to the need for them to work remotely, which also affected their psychological well-being. Several cases of respondents indicated a negative assessment of their own productivity, and thus their quality of work life and sense of psychological well-being. However, some respondents, on the contrary, showed a desire to work, the need for self-improvement, and to continue their mission of teaching.
How the COVID-19 Pandemic Affects Risk Awareness in Dentists: A Scoping Review
Authors: Wolf Thomas Gerhard , de Col Leonardo , Banihashem Rad Seyed Ahmad , Castiglia Paolo , Arghittu Antonella , Cannavale Mina , Campus Guglielmo
Published: April 2022

Dentists are on the frontline of infection, especially when it comes to respiratory viruses like the new coronavirus. The purpose of this paper is to conduct a scoping review to better understand dentists’ risk awareness, awareness of COVID-19 symptoms, preventive measures, and effective methods of COVID-19 infection prevention and management. This paper systematically assesses the published literature on dentistry and COVID-19. Various electronic databases including Ovid MEDLINE, Scopus, Embase, and MEDLINE via PubMed were searched up to 9 September 2021. Overall, 39 papers were included. Almost the entirety of dentists (94.5%) reported awareness of the three most common COVID-19 symptoms, and a risk awareness score of about 90% was shown, while 88.2% of dentists reported adopting preventive measures. More than 50% did not want to treat infected people. While 70.3% of dentists recommended usage of N95 masks, the rate of dentists using them was below 40%. Sufficient awareness of risks during the pandemic was found in dentists. Although they were using preventive measures, there remains upside potential for adopting all recommended measures. Further, the usage of N95 masks is improvable, even though the benefit of wearing them could not be confirmed.
Investigating Relationships between Runoff–Erosion Processes and Land Use and Land Cover Using Remote Sensing Multiple Gridded Datasets
Authors: Fonseca Cláudia Adriana Bueno da , Al-Ansari Nadhir , Silva Richarde Marques da , Santos Celso Augusto Guimarães , Zerouali Bilel , Oliveira Daniel Bezerra de , Elbeltagi Ahmed
Published: April 2022

Climate variability, land use and land cover changes (LULCC) have a considerable impact on runoff–erosion processes. This study analyzed the relationships between climate variability and spatiotemporal LULCC on runoff–erosion processes in different scenarios of land use and land cover (LULC) for the Almas River basin, located in the Cerrado biome in Brazil. Landsat images from 1991, 2006, and 2017 were used to analyze changes and the LULC scenarios. Two simulations based on the Soil and Water Assessment Tool (SWAT) were compared: (1) default application using the standard model database (SWATd), and (2) application using remote sensing multiple gridded datasets (albedo and leaf area index) downloaded using the Google Earth Engine (SWATrs). In addition, the SWAT model was applied to analyze the impacts of streamflow and erosion in two hypothetical scenarios of LULC. The first scenario was the optimistic scenario (OS), which represents the sustainable use and preservation of natural vegetation, emphasizing the recovery of permanent preservation areas close to watercourses, hilltops, and mountains, based on the Brazilian forest code. The second scenario was the pessimistic scenario (PS), which presents increased deforestation and expansion of farming activities. The results of the LULC changes show that between 1991 and 2017, the area occupied by agriculture and livestock increased by 75.38%. These results confirmed an increase in the sugarcane plantation and the number of cattle in the basin. The SWAT results showed that the difference between the simulated streamflow for the PS was 26.42%, compared with the OS. The sediment yield average estimation in the PS was 0.035 ton/ha/year, whereas in the OS, it was 0.025 ton/ha/year (i.e., a decrease of 21.88%). The results demonstrated that the basin has a greater predisposition for increased streamflow and sediment yield due to the LULC changes. In addition, measures to contain the increase in agriculture should be analyzed by regional managers to reduce soil erosion in this biome.
Iron Homeostasis in the CNS: An Overview of the Pathological Consequences of Iron Metabolism Disruption
Authors: Porras Christina A. , Rouault Tracey A.
Published: April 2022

Iron homeostasis disruption has increasingly been implicated in various neurological disorders. In this review, we present an overview of our current understanding of iron metabolism in the central nervous system. We examine the consequences of both iron accumulation and deficiency in various disease contexts including neurodegenerative, neurodevelopmental, and neuropsychological disorders. The history of animal models of iron metabolism misregulation is also discussed followed by a comparison of three patients with a newly discovered neurodegenerative disorder caused by mutations in iron regulatory protein 2.
Specific Amino Acid Substitutions in OXA-51-Type β-Lactamase Enhance Catalytic Activity to a Level Comparable to Carbapenemase OXA-23 and OXA-24/40
Authors: Chan Kwan-Wai , Liu Chen-Yu , Wong Ho-Yin , Chan Wai-Chi , Wong Kwok-Yin , Chen Sheng
Published: April 2022

The chromosomal blaOXA-51-type gene encodes carbapenem-hydrolyzing class D β-lactamases (CHDLs), specific variants shown to mediate carbapenem resistance in the Gram-negative bacterial pathogen Acinetobacter baumannii. This study aims to characterize the effect of key amino acid substitutions in OXA-51 variants of carbapenem-hydrolyzing class D β-lactamases (CHDLs) on substrate catalysis. Mutational and structural analyses indicated that each of the L167V, W222G, or I129L substitutions contributed to an increase in catalytic activity. The I129L mutation exhibited the most substantial effect. The combination of W222G and I129L substitutions exhibited an extremely strong catalytic enhancement effect in OXA-66, resulting in higher activity than OXA-23 and OXA-24/40 against carbapenems. These findings suggested that specific arrangement of residues in these three important positions in the intrinsic OXA-51 type of enzyme can generate variants that are even more active than known CHDLs. Likewise, mutation leading to the W222M change also causes a significant increase in the catalytic activity of OXA-51. blaOXA-51 gene in A. baumannii may likely continue to evolve, generating mutant genes that encode carbapenemase with extremely strong catalytic activity.
Multiple Roles of SMC5/6 Complex during Plant Sexual Reproduction
Authors: Yang Fen , Pecinka Ales
Published: April 2022

Chromatin-based processes are essential for cellular functions. Structural maintenance of chromosomes (SMCs) are evolutionarily conserved molecular machines that organize chromosomes throughout the cell cycle, mediate chromosome compaction, promote DNA repair, or control sister chromatid attachment. The SMC5/6 complex is known for its pivotal role during the maintenance of genome stability. However, a dozen recent plant studies expanded the repertoire of SMC5/6 complex functions to the entire plant sexual reproductive phase. The SMC5/6 complex is essential in meiosis, where its activity must be precisely regulated to allow for normal meiocyte development. Initially, it is attenuated by the recombinase RAD51 to allow for efficient strand invasion by the meiosis-specific recombinase DMC1. At later stages, it is essential for the normal ratio of interfering and non-interfering crossovers, detoxifying aberrant joint molecules, preventing chromosome fragmentation, and ensuring normal chromosome/sister chromatid segregation. The latter meiotic defects lead to the production of diploid male gametes in Arabidopsis SMC5/6 complex mutants, increased seed abortion, and production of triploid offspring. The SMC5/6 complex is directly involved in controlling normal embryo and endosperm cell divisions, and pioneer studies show that the SMC5/6 complex is also important for seed development and normal plant growth in cereals.
Identification of TALE Transcription Factor Family and Expression Patterns Related to Fruit Chloroplast Development in Tomato (Solanum lycopersicum L.)
Authors: Wang Jin , Zhao Pan , Cheng Baohui , Zhang Yanhong , Shen Yuanbo , Wang Xinyu , Zhang Qinghua , Lou Qianqi , Zhang Shijie , Wang Bo , Qi Shiming , Li Yushun , Islam Md. Monirul , Muhammad Tayeb , Zhang Fei , Liang Yan
Published: April 2022

The TALE gene family is an important transcription factor family that regulates meristem formation, organ morphogenesis, signal transduction, and fruit development. A total of 24 genes of the TALE family were identified and analyzed in tomato. The 24 SlTALE family members could be classified into five BELL subfamilies and four KNOX subfamilies. SlTALE genes were unevenly distributed on every tomato chromosome, lacked syntenic gene pairs, and had conserved structures but diverse regulatory functions. Promoter activity analysis showed that cis-elements responsive to light, phytohormone, developmental regulation, and environmental stress were enriched in the promoter of SlTALE genes, and the light response elements were the most abundant. An abundance of TF binding sites was also enriched in the promoter of SlTALE genes. Phenotype identification revealed that the green shoulder (GS) mutant fruits showed significantly enhanced chloroplast development and chlorophyll accumulation, and a significant increase of chlorophyll fluorescence parameters in the fruit shoulder region. Analysis of gene expression patterns indicated that six SlTALE genes were highly expressed in the GS fruit shoulder region, and four SlTALE genes were highly expressed in the parts with less-developed chloroplasts. The protein-protein interaction networks predicted interaction combinations among these SlTALE genes, especially between the BELL subfamilies and the KNOX subfamilies, indicating a complex regulatory network of these SlTALE genes in chloroplast development and green fruit shoulder formation. In conclusion, our result provides detailed knowledge of the SlTALE gene for functional research and the utilization of the TALE gene family in fruit quality improvement.
Human α-Defensin-6 Neutralizes Clostridioides difficile Toxins TcdA and TcdB by Direct Binding
Authors: Barthold Lara , Heber Sebastian , Schmidt Christoph Q. , Gradl Marion , Weidinger Gilbert , Barth Holger , Fischer Stephan
Published: April 2022

Rising incidences and mortalities have drawn attention to Clostridioides difficile infections (CDIs) in recent years. The main virulence factors of this bacterium are the exotoxins TcdA and TcdB, which glucosylate Rho-GTPases and thereby inhibit Rho/actin-mediated processes in cells. This results in cell rounding, gut barrier disruption and characteristic clinical symptoms. So far, treatment of CDIs is limited and mainly restricted to some antibiotics, often leading to a vicious circle of antibiotic-induced disease recurrence. Here, we demonstrate the protective effect of the human antimicrobial peptide α-defensin-6 against TcdA, TcdB and the combination of both toxins in vitro and in vivo and unravel the underlying molecular mechanism. The defensin prevented toxin-mediated glucosylation of Rho-GTPases in cells and protected human cells, model epithelial barriers as well as zebrafish embryos from toxic effects. In vitro analyses revealed direct binding to TcdB in an SPR approach and the rapid formation of TcdB/α-defensin-6 complexes, as analyzed with fluorescent TcdB by time-lapse microscopy. In conclusion, the results imply that α-defensin-6 rapidly sequesters the toxin into complexes, which prevents its cytotoxic activity. These findings extend the understanding of how human peptides neutralize bacterial protein toxins and might be a starting point for the development of novel therapeutic options against CDIs.
Generation of Cancer Stem/Initiating Cells by Cell–Cell Fusion
Authors: Dittmar Thomas
Published: April 2022

CS/ICs have raised great expectations in cancer research and therapy, as eradication of this key cancer cell type is expected to lead to a complete cure. Unfortunately, the biology of CS/ICs is rather complex, since no common CS/IC marker has yet been identified. Certain surface markers or ALDH1 expression can be used for detection, but some studies indicated that cancer cells exhibit a certain plasticity, so CS/ICs can also arise from non-CS/ICs. Another problem is intratumoral heterogeneity, from which it can be inferred that different CS/IC subclones must be present in the tumor. Cell–cell fusion between cancer cells and normal cells, such as macrophages and stem cells, has been associated with the generation of tumor hybrids that can exhibit novel properties, such as an enhanced metastatic capacity and even CS/IC properties. Moreover, cell–cell fusion is a complex process in which parental chromosomes are mixed and randomly distributed among daughter cells, resulting in multiple, unique tumor hybrids. These, if they have CS/IC properties, may contribute to the heterogeneity of the CS/IC pool. In this review, we will discuss whether cell–cell fusion could also lead to the origin of different CS/ICs that may expand the overall CS/IC pool in a primary tumor.