bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Antibiotic Prophylaxis in a Retrospective Cohort of Hospitalized Patients with COVID-19 Treated with the Combination of Steroids and Tocilizumab
Authors: Membrillo de Novales Francisco Javier , Ramirez-Olivencia German , Mata Forte Tatiana , Zamora Cintas María Isabel , Simón Sacristán María , Sánchez de Castro María , Estébanez Muñoz Miriam
Published: August 2023

Objectives:
In the context of COVID-19, patients with severe or critical illness may be more susceptible to developing secondary bacterial infections. This study aims to investigate the relationship between the use of prophylactic antibiotic therapy and the occurrence of bacterial or fungal isolates following the administration of tocilizumab in hospitalized COVID-19 patients who had previously received steroids during the first and second waves of the pandemic in Spain.
Methods:
This retrospective observational study included 70 patients hospitalized with COVID-19 who received tocilizumab and steroids between January and December 2020. Data on demographics, comorbidities, laboratory tests, microbiologic results, treatment, and outcomes were collected from electronic health records. Patients were divided into two groups based on the use of antibiotic prophylaxis, and the incidence of bacterial and fungal colonizations/infections was analyzed.
Results:
Among the included patients, 45 patients received antibiotic prophylaxis. No significant clinical differences were observed between patients based on prophylaxis use regarding the number of clinically diagnosed infections, ICU admissions, or mortality rates. However, patients who received antibiotic prophylaxis showed a higher incidence of colonization by multidrug-resistant germs compared to the subgroup that did not receive prophylaxis. The most commonly isolated microorganisms were Candida albicans, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis.
Conclusions:
In this cohort of hospitalized COVID-19 patients treated with tocilizumab and steroids, the use of antibiotic prophylaxis did not reduce the incidence of secondary bacterial infections. However, it was associated with an increased incidence of colonization by multidrug-resistant organisms.
Exposure to Bisphenol A and its analogs among Thai School-age Children
Authors: Numsriskulrat Nattakarn , Teeranathada Thanawan , Bongsebandhu-phubhakdi Chansuda , Aroonparkmongkol Suphab , Choi Kyungho , Supornsilchai Vichit
Published: August 2023

Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as substitutes for bisphenol A (BPA) in the plastic industry, driven by concerns over the adverse effects of BPA. However, there is a lack of infor-mation on the extent of exposure to these chemicals in children. This study aims to assess the extent of BPA, BPF, and BPS exposure and determine factors that influence such exposure. A group of Thai children (age 6-13 years, n=358) were recruited between October 2019-2020. Two first morning void was collected one week apart. Demographic and exposure-related information was gathered. Urinary concentrations of bisphenols were analyzed by liquid-chromatography and tandem mass spectrometry. Correlation between bisphenol concentrations and various factors was determined using generalized estimating equations with linear model. BPA, BPF, and BPS were detected at 79.6%, 31.0%, and 16.8%, with geometric mean (GM) concentrations of 1.41, 0.013, and 0.014 ng/mL respectively. Younger children aged < 10 years exhibited 1.3-1.6 times higher GM levels of all bisphenols compared to older children. Exposure to food stored in plas-tic containers were associated with higher levels of BPF and BPS. In conclusion, BPA was the most fre-quently detected bisphenol in urine samples from Thai children, followed by BPF and BPS.
User Perceptions of Visual Blood: An International Mixed Methods Study on Novel Blood Gas Analysis Visualization
Authors: Gasciauskaite Greta , Lunkiewicz Justyna , Schweiger Giovanna , Budowski Alexandra D , Henckert David , Roche Tadzio R , Bergauer Lisa , Meybohm Patrick , Hottenrott Sebastian , Zacharowski Kai , Raimann Florian Jürgen , Rivas Eva , López-Baamonde Manuel , Ganter Michael T , Schmidt Tanja , Nöthiger Christoph B , Tscholl David W , Akbas Samira
Published: August 2023

Blood gas analysis plays a central role in modern medicine. Advances in technology have expanded the range of available parameters and increased the complexity of their interpretation. By applying user-centered design principles, it is possible to reduce the cognitive load associated with interpreting blood gas analysis. In this international, multicenter study, we explored anesthesiologists' perspectives on Visual Blood, a novel visualization technique for presenting blood gas analysis results. We conducted interviews with participants following two computer-based simulation studies: the first utilizing Virtual Reality (VR) (50 participants) and the second without VR (70 participants). Employing the template approach, we identified key themes in the interview responses and formulated six statements, which were rated using Likert scales: from 1 (strongly disagree) to 5 (strongly agree) in an online questionnaire. The most frequently mentioned theme was the positive usability features of Visual Blood. The online survey revealed that participants found Visual Blood to be an intuitive method for interpreting blood gas analysis (median 4, IQR 4-4, p<0.001). Participants noted that minimal training was required to effectively learn how to interpret Visual Blood (median 4, IQR 4-4, p<0.001). However, adjustments are necessary to reduce visual overload (median 4, IQR 2-4, p<0.001). Overall, Visual Blood received a favorable response. The strengths and weaknesses derived from these data will help optimize future versions of Visual Blood to improve the presentation of blood gas analysis results.
Exploring the Impact of Smartphone Addiction on Risk Decision-Making Behavior among College Students Based on fNIRS Technology
Authors: Liu Xiaolong , Tian Ruiyi , Liu Huafang , Bai Xue , Lei Yi
Published: August 2023

Smartphone Addiction is a social issue caused by excessive smartphone use, affecting decision-making processes. Current research on the risky decision-making abilities of smartphone addicts is limited. This study used the fNIRS brain imaging technique and a Sequential Risk-Taking Task experimental paradigm to investigate the decision-making behavior and brain activity of smartphone addicts under varying risk levels. Using a mixed experimental design, the research assessed decision-making ability and brain activation levels as dependent variables across two groups (addiction and control), two risk amounts (high and low), and two outcomes (gain and loss). The study included 42 participants, with 25 in the addiction group and 17 in the control group. Results indicated that risk level significantly impacted the decision-making ability of smartphone addicts, with high-risk levels leading to weaker decision-making ability and increased risk-taking. However, at low risk levels, decision-making abilities between addicts and healthy individuals showed no significant difference. Furthermore, brain imaging results using fNIRS revealed stronger brain activation in the dlPFC region for smartphone addicts under loss outcome conditions, with no significant differences between the two groups in terms of brain activation at varying risk volumes. These findings are critical in promoting healthy smartphone use, guiding clinical treatment, and advancing brain mechanism research.
Fine Structure of Solar Metric Radio bursts: ARTEMIS-IV/JLS and NRH Observations
Authors: Alissandrakis Costas , Hillaris Alexander E. , Bouratzis Costas , Armatas Spyros
Published: August 2023

Radio bursts provide important diagnostics of energetic phenomena of the Sun. In particular, bursts in decimetric and metric wavelengths probe the physical conditions and the energy release processes in the low corona as well as their association with heliospheric phenomena. The advent of spectral radio data with high time and high frequency resolution has provided a wealth of information on phenomena of short duration and narrow bandwidth. Of particular value are spectral data combined with imaging observations at specific frequencies. In this work we briefly review the results of a series of observations comprised from high-sensitivity, low-noise dynamic spectra obtained with the acousto-optic analyzer (SAO) of the ARTEMIS-IV/JLS solar radiospectrograph, in conjunction with high time-resolution images from the Nançay Radioheliograph (NRH). Our studies include fine structures embedded in type IV burst continua (mostly narrow-band "spikes" and intermediate drift "fiber" bursts) and spike-like structures detected near the front of type-II bursts. The implications of the observational results to theoretical models are discussed.
Characteristics of Filipino Online Learners: A Survey of Science Education Students' Engagement, Self-Regulation, and Self- Efficacy
Authors: Briones Mary Rose , Prudente Maricar , Errabo Denis Dyvee
Published: August 2023

Online education allows learners to develop knowledge and skills flexibly and conveniently—such observation among students whose characteristics involve student engagement, self-regulation, and self-efficacy. However, studies to characterize Filipino online learners seem lacking. Thus, this study aimed to characterize science education tertiary students in the Philippines concerning their online student engagement (OSE), self-regulated learning (SRL), and online learning self-efficacy (OLSE). The unprecedented events brought by COVID-19 pandemic also urged the implementation of online modalities while there is no available information on students’ online learning profiles. Hence, researchers used a survey research employed through ex post facto approach to determine the effects of the demographic profile on OSE, SRL, and OLSE. The survey was participated by N=373 respondents who answered the questionnaire with informed consent administered via Google Forms. Results revealed that OSE indicators moderately characterized students, while SRL and OLSE indicators are true of them, substantiated by the overall mean M=3.85(SD=0.90), M=3.86(SD=0.92), and M=3.14(SD=0.73), respectively. Also, multivariate tests showed no significant effect among the independent groups (p>0.05), except for gender and OLSE interaction (p<0.05) so, only in OLSE was a significant difference found in gender. In conclusion, Filipino online learners are moderate across aspects of student engagement, self-regulation, and self-efficacy.
Intravenous Administration of Human Muse Cells Ameliorates Deficits in a Rat Model of Subacute Spinal Cord Injury
Authors: Takahashi Yoshiharu , Kajitani Takumi , Endo Toshiki , Nakayashiki Atsushi , Inoue Tomoo , Niizuma Kuniyasu , Tominaga Teiji
Published: August 2023

Background Multilineage-differentiating stress-enduring (Muse) cells are newly established pluripotent stem cells. The aim of the present study was to examine the potential of the systemic administration of Muse cells as an effective treatment for subacute SCI.
Methods: We intravenously administered the clinical product “CL2020” containing Muse cells to a rat model two weeks after mid-thoracic spinal cord contusion. Eight experimental animals received CL2020 and 12 received the vehicle. Behavioral analyses were conducted over 20 weeks. Histological evaluations were performed. After 20 weeks of observation, diphtheria toxin was administered to 3 CL2020-treated animals to selectively ablate human cell functions.
Results: Hindlimb motor functions significantly improved from 6 to 20 weeks after the administration of CL2020. The cystic cavity was smaller in the CL2020 group. Furthermore, larger numbers of descending 5-HT fibers were preserved in the distal spinal cord. Muse cells in CL2020 were considered to have differentiated into neuronal and neural cells in the injured spinal cord. Neuronal and neural cells were identified in the gray and white matter, respectively. Importantly, these effects were reversed by the selective ablation of human cells by diphtheria toxin.
Conclusions: Intravenously administered Muse cells facilitated the therapeutic potential of CL2020 for severe subacute spinal cord injury.
A Review of the Network Arch Bridge
Authors: Danciu Alexandra Denisa , Guțiu Ștefan I. , Moga Catalin , Dragomir Mihai Liviu , Ciotlaus Madalina , Marusceac Vladimir
Published: August 2023

The first Network Arch Bridge (NAB) was opened to traffic 60 years ago and since then the idea spread around the world. It is a type of a tied arch bridge that combines the benefits of tied arch bridges and trusses in a single system, thus it has a very good stiffness even in the case of extremely slender structures. In a Network Arch the hangers are inclined and some of them intersect each other at least twice. The core of a NAB is the hanger arrangement that minimizes the bending moment in the arch to very small values, leading thus to compression in the arch. This paper reviews the Network Arch idea devised by prof. Tveit and extended by researchers and engineers worldwide. A comprehensive review of the literature about the Network Arch is provided and the situation of NABs around the world from the standpoint of location, span, structural system used for the cross-section, bridge type (road, railway, combined or pedestrian) and opening year is discussed.
Benefits of Urban Forest Healing Program on Depression and Anxiety Symptoms in Depressive Patients
Authors: Yeon Poung-Sik , Kang Si-Nae , Lee Nee-Eun , Kim In-Ok , Min Gyeong-Min , Kim Ga-Yeon , Kim Jin-Gun , Shin Won-Sop
Published: August 2023

Depression is considered a widespread mental health problem worldwide. Moreover, anxiety symptoms are very closely related to depression patients, and it is known that the coexistence rate of depression and anxiety diagnosed simultaneously is high. Treatment and preventive management of depression and anxiety are essential for public health. Forest healing is attracting attention as a form of low-cost preventive medicine that is safe and has no side effects. However, although the physiological and psychological effects have been scientifically proven, it is insufficient to reveal a direct relationship between forest healing and depression. This study investigated the benefits of an urban forest healing program on depression and anxiety symptoms of depressive disorders. We employed a randomized controlled trial design. Forty-seven depression patients were randomly divided into an urban forest healing program group and a control group. Measures included the Montgomery-Asberg depression rating scale (MADRS), the Hamilton Anxiety Rating Scale (HARS), and the State-Trait Anxiety Inventory (STAI) questionnaires. Our results revealed that the combination of general treatment and forest healing programs for patients with depression is more effective in improving depression and anxiety than routine treatment alone. We expect our work to serve as a starting point for more sophisticated research in discussing the availability of non-pharmacological treatments in forest healing.
Exploring the Significance of Gut Microbiota in Cardiovascular Health
Authors: Akter Shamima , Choubey Mayank , Arbee Shahida , Mohib Mohammad Mohabbulla , Tirumalasetty Munichandra B. , Minhaz Naofel , Akhtar Asif , Bismee Nadera Naquib , Mohiuddin Mohammad Sarif
Published: August 2023

Cardiovascular disease (CVD), comprising heart and blood vessel disorders, persists as the foremost contributor to global morbidity and mortality. In modern times, the intricate composition of gut microbiota has garnered significant focus, particularly for its varying impact on diverse ailments. Perturbations in gut microbiota and consequent dysbiosis have demonstrated connections with the advancement and origin of CVD, including conditions like atherosclerosis, hypertension, and heart failure. This comprehensive review delves into the pivotal role of gut microbiota in maintaining cardiovascular well-being.
Recognition of Cancer With Bipartite and Path as Neutrosophic SuperHyperGraph By the Tool Called Stability
Authors: Garrett Henry
Published: August 2023

New ideas on the framework of Neutrosophic SuperHyperGraph for different styles of Neutrosophic SuperHyper-Bipartite and Neutrosophic SuperHyper-Path are introduced. More instances and more clarifications alongside sufficient references.
Ebola Virus Infection of Liver Cells: Effect of Interferon Gamma on Virus Infection of Primary Kupffer Cells and a Kupffer Cell Line
Authors: Aguilar-Briseño José A. , Elliff Jonah M. , Patten J. J. , Wilson Lindsay R. , Davey Robert A. , Bailey Adam L. , Maury Wendy J.
Published: August 2023

Ebola virus disease (EVD) represents a global health threat. The etiological agents of EVD are 6 species of Orthoebolaviruses, with Orthoebolavirus zairense (EBOV) having the greatest public health and medical significance. EVD disease pathogenesis occurs as a result of broad cellular tropism of the virus, robust viral replication and a potent and dysregulated production of cytokines. In vivo, tissue macrophages are some of the earliest cells infected and contribute significantly to virus load and cytokine production. While EBOV is known to infect macrophages and to generate high titer virus in the liver, EBOV infection of liver macrophages, Kupffer cells, has not previously been examined in tissue culture or experimentally manipulated in vivo. Here, we employed primary murine Kupffer cells (KC) and an immortalized murine Kupffer cell line (ImKC) to assess EBOV-eGFP replication in liver macrophages. KCs and ImKCs were highly permissive for EBOV infection and IFN-γ polarization of these cells suppressed their permissiveness to infection. The kinetics of IFN-γ-elicited antiviral responses were examined using a biologically-contained model of EBOV infection termed EBOV ΔVP30. The antiviral activity of IFN-γ was transient, but a modest ~3-fold reduction of infection persisted for as long as 6 days post treatment. To assess the interferon stimulated gene products (ISGs) responsible for protection, the efficacy of secreted ISGs induced by IFN-γ were evaluated. Secreted ISGs blocked recombinant VSV expressing EBOV GP (rVSV/EBOV GP) infection, but failed to block EBOV ΔVP30. Our studies define new cellular tools for the study of EBOV infection that can potentially aid the development of new antiviral therapies. Furthermore, our data underscore the importance of macrophages in EVD pathogenesis and those IFN-γ-elicited ISGs that help to control EBOV infection.
A Study on the Calculation of TEQ and MEQ of 16 PAHs in Kuwait’s Crude Oil-Contaminated Soil and Ecotoxicity Assessment of Bioluminescent Bacteria
Authors: Lee Jaemin , Choi Byeongwook , Yang Haein , Lee Kyoungjin , Heo Taeyeong , Jho Eunhea , Song Deokjong , Lee Sungjong
Published: August 2023

The oil spilled from the Gulf war caused land pollution and various petroleum compounds. Among petroleum compounds, polycyclic aromatic hydrocarbons (PAHs) are known for toxicity, and have been designated as carcinogenic, mutagenic, and endocrine disrupting substances by the United States Environmental Protection Agency (USEPA). According to the USEPA, toxic equivalents quotient (TEQ), and mutagenic equivalents quotient (MEQ) can be calculated by multiplying the toxic equivalency factor (TEF) and mutagenic equivalency factor (MEF) by the concentration of PAHs. The USEPA calculates the relative TEF for other PAHs by setting the TEF value of benzo(a)pyrene, which is highly toxic, to 1. Relative MEF is also calculated in the same way. In this study, the TPH concentrations of crude oil-contaminated soils collected from the Burgan in Kuwait were measured to be 5, 8, and 20% and TEQ and MEQ of the soils were calculated by multiplying the 16 PAHs concentrations measured for each sample by TEF and MEF, respectively. When the TPH concentration was increased by 4 times from 5% to 20%, the TEQ was increased by about 9 times and the MEQ was increased by about 10 times. Therefore, it was concluded that as the TPH concentration increases, the carcinogenicity and mutation rate increase greatly. In addition, ecotoxicity assessment was performed using luminescent bacteria to compare the relationship between the calculated TEQ and MEQ and actual ecotoxicity, and benzo(a)pyrene among 16 PAHs showed a coefficient of determination of 0.95. In this study, ecotoxicity in PAHs-contaminated environment can be estimated by analyzing benzo(a)pyrene as a representative substance.
Novel In Vitro Models for Cell Differentiation and Drug Transport Studies of the Human Intestine
Authors: Przybylla Randy , Krohn Mathias , Sellin Marie-Luise , Frank Marcus , Oswald Stefan , Linnebacher Michael
Published: August 2023

Still, the most common in vitro model for absorption, distribution, metabolism and excretion (ADME) purposes is the Caco-2 cell line. However, clear differences in gene- and protein expression towards the small intestine and an at best fair prediction accuracy of intestinal drug absorption restrict the usefulness as model for intestinal epithelial cells. To overcome these limitations, we evaluated a panel of low-passaged patient-derived colorectal cancer cell lines of the HROC collection concerning similarities to small intestinal epithelial cells and their potential to predict intestinal drug absorption. After initial screen of a larger panel, ten cell lines with confluent outgrowth and long-lasting barrier forming potential were further characterized in close detail. Tight junctional complexes and microvilli structures were detected in all lines, differentiated epithelial cells were abundant in 7/10. All lines expressed multiple transporter molecules, with expression levels in three lines being close to those of small intestinal epithelial cells. Compared to the Caco-2 model, three HROC lines demonstrated both higher similarity to jejunal epithelial tissue cells and higher regulatory potential of relevant drug transporters. In sum, these lines would be better suited human small intestinal epithelium models for basic and translational research; especially for ADME studies.
Elevated Arterial Blood Pressure as a Delayed Complication Following COVID-19 -Systemic Review
Authors: Bielecka Emilia , Sielatycki Piotr , Pietraszko Paulina , Zapora-Kurel Agnieszka , Zbroch Edyta
Published: August 2023

Hypertension is one of the most common and significant cardiovascular risk factor. With the emergence of COVID-19, concerns have arisen about its potential to exacerbate cardiovascular morbidity and mortality. Several theories have emerged linking hypertension with COVID-19. However, the relationship between SARS-CoV-2 infection and long-term risk of hypertension remains insufficiently explored. Therefore, our study aimed to investigate the impact of COVID-19 infection on blood pressure and long-term risk of hypertension developing. A comprehensive search was conducted in the PubMed and SCOPUS databases up to 10th August 2023 for relevant papers. The analysis included 35 eligible articles. The majority of the latest studies pointed to increase BP after COVID-19 infection. However, several observations contradicted the influence of SARS-CoV-2 on the hypertension development or suggesting a transient effect. The possible role of the loss of angiotensin-converting enzyme 2, cytokine storm, increased sympathetic activity, and endothelial dysfunction were considered. On the other hand, BP changes following COVID-19 could be a consequence of lifestyle changes, which often accompanied COVID-19 infection. Our findings highlight the urgent need for comprehensive research on the COVID-19 and hypertension link. These insights are crucial for implementing effective prevention and management strategies for individuals with the history of COVID-19 infection.
Thermal Effects in Ising Cosmology
Authors: Irges Nikos , Kalogirou Antonis , Koutroulis Fotis
Published: August 2023

We consider a real scalar field in de Sitter background and compute its thermal propagators.
We propose that in a dS/CFT context, non-trivial thermal effects as seen by an `out' observer can be encoded in the anomalous dimensions of the d=3 Ising model.
One of these anomalous dimensions, the critical exponent eta, fixes completely a number of cosmological observables, which we compute.
Kinetic Investigation of Oxidative Thermal Decomposition of Levonorgestrel
Authors: Ridichie Amalia , Ledeţi Adriana , Peter Francisc , Ledeţi Ionuţ , Muntean Cornelia , Rădulescu Matilda
Published: August 2023

In this study, we have focused on studying the heterogenous degradation kinetics of regarding the decomposition of emergency contraceptive agent levonorgestrel (LNG), which is a second-generation synthetic progestogen that is the active component of the racemic mixture of norgestrel. The degradation processes of the active pharmaceutical ingredient (API) were compared with the ones obtained from model system containing the API along with the excipients that are found in a commercialized pharmaceutical formulation in a mass ratio of 1:1 (LNGMIX), in order to observe if the excipients have a stabilizing or destabilizing effect for the degradation of this progestogen. To achieve this, the following investigational methods were used: FTIR (Fourier transform infrared) spectroscopy and thermal analysis (TG/DTG/DSC analysis). For the kinetic analysis the data obtained from two main decomposition processes observed on the DTG curves were used and processed with a preliminary method, namely ASTM E698 and two isoconversional methods: Friedman and Flynn-Wall-Ozawa. The isoconversional study revealed that the decomposition mechanism of both LNG and LNGMIX are complex, and the excipients have a stabilizing effect over decomposition of API in tablet.
The Impact of Avoidable Mortality Changes on Life Expectancy at Birth in Korea, 2000-2020
Authors: Lee Shi Nae , Lim Dar Oh , Lee Soo Hyun
Published: August 2023

This study investigated mortality discrepancies in Korea, 2000-2020, with a focus on age, gender, and preventable causes of death, and their impact on life expectancy at birth. The study utilized data from Korean Standard Classification of Diseases and OECD/Eurostat lists of preventable and treatable causes of death and employed Arriaga's method. Noteworthy findings revealed gender disparities in life expectancy increases, with male gaining an advantage over females from 2000-2020 (8.142 years for males versus 6.806 years for females). The most significant increase in life expectancy was found to be in males aged 70-74 and females aged 75-79. The gender gap in LEB is narrowing and that the main age group contributing to the gender gap are getting older. Reduced gains in life expectancy at birth resulted from avoidable mortality, contributing 57.3% to the increase. Cerebrovascular disease and transport accidents were positive contributors, whereas suicide and certain cancers were negative contributors. The changes in mortality for individuals aged 65 and over had an impact of 45.44% (males) and 71.01% (females) on life expectancy gains. This study offers a novel approach in evaluating avoidable mortality and life expectancy, having the potential to improve international health indicators and inform health policies.
The Risk of Drug Interactions in Older Primary Care Patients after Hospital Discharge; the Role of Drug Reconciliation
Authors: Vocca Cristina , Siniscalchi Antonio , Rania Vincenzo , Galati Cecilia , Marcianò Gianmarco , Palleria Caterina , Catarisano Luca , Gareri Ilaria , Leuzzi Marco , Muraca Lucia , Citraro Rita , Nanci Giacinto , Scuteri Antonio , Bianco Rosa Candida , Fera Iolanda , Greco Antonietta , Leuzzi Giacomo , De Sarro Giovambattista , D'Agostino Bruno , Gallelli Luca
Published: August 2023

Introduction: Drug interactions (DDIs) represent an important clinical problem particularly in elderly patients due to polytherapy, comorbidity and physiological change in pharmacodynamic and pharmacokinetic pathways. In this study we investigated the association between drugs prescribed during hospital or clinical room discharge and the development of DDIs with drugs daily used from each patient.
Methods: we performed an observational, retrospective, multicenter study on medical records of outpatients referred to general practitioners. Drug interactions (DDIs) were measured using the drug interactions probability scale. Collected data were analyzed using the Statistical Package for the Social Sciences. Results: During the study we evaluated 1,622 medical records. We recorded the development of DDIs in 10.3% of patients, of those 11.6% induced the hospitalization. Logistic regression showed an association between DDIs and sex and number of drugs used (P = 0.023). Conclusion: this real-life study shows that DDIs are common in elderly patients and physicians must take more attention after hospital discharge revaluating the treatment in order to reduce the risk of DDIs and hospitalization.
Seroprevalence of Viral Enzootic Diseases in Backyard Farms in Serbia
Authors: Milicevic Vesna , Glišić Dimitrije , Zurovac Sapundzic Zorana , Milovanovic Bojan , Maletić Jelena , Jezdimirovic Nemanja , Kureljušić Branislav
Published: August 2023

Contrary to pig farming in developed western countries, in a large part of the world, pigs are still traditionally kept, in small backyard farms, usually for family needs. Their main characteristics are low biosecurity, swill feeding, natural breeding and uncontrolled trade. Given the high number of backyard farms in Serbia and the risk they are thought to pose to intensive pig farming, the main aim of this study was to evaluate the prevalence of major viral diseases of swine among traditionally kept pigs in small holdings with low biosecurity. For this investigation, 222 serum samples from 69 backyard holdings were randomly selected and tested for PRRS, Aujeszky`s disease, PPV infection and Swine influenza by ELISA. The herd-level seroprevalence of PRRS, Aujeszky`s disease and PPV infection was 2.9%, 27.5% and 37.7%, respectively. Swine influenza seroconversion was not confirmed in any of the tested holdings. Despite widely distributed PPV and AD in backyard farms in Serbia, almost 50% of them are still negative for the all-tested disease. The backyard farms must be monitored, and owners educated as their role in eradication programs and obtaining country-free status may be crucial.
Polysaccharide-Stabilized Pd-Ag Nanocatalysts for Hydrogenation of 2-Hexyn-1-Ol
Authors: Zharmagambetova Alima Kainekeyevna , Talgatov Eldar Talgatuly , Auyezkhanova Assemgul Seitkhanovna , Bukharbayeva Farida Umarovna , Jumekeyeva Aigul Iembergenovna
Published: August 2023

A new one-pot technique for preparation of polysaccharide-based Pd- and Pd-Ag nanocatalysts by sequential supporting natural polymer (2-hydroxyethyl cellulose (HEC), chitosan (Chit), pectin (Pec)) and metals on zinc oxide was developed. The nanocatalysts based on polysaccharide were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer–Emmett–Teller (BET), infrared spectroscopy (IRS), X-ray powder diffraction (XRD), X-ray photoelectron spectra (XPS) and elemental analysis methods. The catalyst characterization results indicated complete adsorption of polysaccharide and metal ions on zinc oxide, forming polymer-stabilized Pd nanoparticles of ~2 nm in size, evenly distributed on the support surface. The catalysts were studied in the hydrogenation of 2-hexyn-1-ol under mild conditions (0.1 MPa, 40 °C). The catalysts demonstrated nearly the same conversion of 2-hexyn-1-ol. The selectivity to cis-hexen-1-ol of the catalysts decreases in the following order: 0.5%Pd-Ag(3:1)HEC/ZnO>0.5%Pd-Ag(3:1)Pec/ZnO>0.5%Pd-Ag(3:1)Chit/ZnO. The optimum reaction temperature and catalyst loading for the Pd-Ag catalysts modified with HEC and Chit have been determined (40 °C, 0.05 g).
Mechanisms and Adsorption Capacities of Natural and Eco-friendly Nanoparticles for Heavy Metals and Phosphate Ions from Water Using the DSD Approach
Authors: Govedarica Jovana Jokić , Pilipović Dragana Tomašević , Gvoić Vesna , Kerkez Đurđa , Maćerak Anita Leovac , Slijepčević Nataša , Kozma Gábor , Tomin Milena Bečelić
Published: August 2023

The creation of nano-zero-valent iron (nZVI) by implementing plant extracts is an environmentally friendly process. High antioxidant capacity and phenol content indicated the possibility of oak-nZVI synthesis using oak leaf extract as a stable material with minimal agglomeration. Simultaneously removal of Cd and phosphates and Ni and phosphates was optimized by a statistically designed experiment with a definitive screening design. In terms of significance, 4 input parameters on process productivity were monitored: initial metal concentration (1 - 9 mgL-1), initial ion concentration (1- 9 mgL-1), pH value (2 - 10) and oak-nZVI dosage (2 - 16 ml). Phosphate removal efficiency, in the presence of cadmium, was the most influenced by oak-nZVI dose and cadmium concentration, while pH gave three statistically significant interactions with oak-nZVI dosage, cadmium concentration, and phosphate concentration. Phosphate removal efficiency, in the presence of Ni, is the most influenced by nickel concentration, phosphate concentration, pH, two-factor interaction between nZVI dose and Ni concentration, and quadratic phosphate interaction. The process optimization yielded the highest simultaneous removal efficiency of 98.99% and 87.30% for cadmium and phosphate ions, respectively. Also, the highest simultaneous removal efficiency of nickel and phosphates ion was 93.44% for 96.75%, respectively. The optimization process fits into the confidence intervals, confirming the assumption that the adopted regression model describes the process well. This work demonstrated an enormous potential and prosperous application for the Cd(II), Ni(II), and phosphate removal from water matrices.
Factors Influencing Purchase Intention of Organic Food among Individuals with Chronic Diseases
Authors: Limbu Yam B. , McKinley Christopher , Ganesan P. , Wang Tianfu , Zhang Junzhou
Published: August 2023

The objectives of this study are threefold. First, we evaluate the applicability of the information–motivation–behavioral skills (IMB) model for predicting organic food purchase intention (OFPI) in adults with chronic conditions. Second, we examine the indirect effects of organic food knowledge, attitudes toward organic food purchase, and subjective norms on OFPI through self-efficacy. Third, we examine whether these indirect effects are moderated by gender and educational attainment. Data were collected from Indian adults with chronic conditions using a self-administered questionnaire. The results show significant indirect effects of organic food knowledge, attitude toward organic food purchase, and subjective norm on OFPI through self-efficacy. Moreover, the mediating effect of knowledge was moderated by gender and education attainment, with the effect being stronger for females and among individuals with a lower level of education. Organic food marketers, social marketers, and public health agencies promoting organic food consumption to people with chronic conditions should aim to increase their confidence in comprehending organic food. This study contributes to the literature by assessing the applicability of Fisher and Fisher’s (1992) IMB model in understanding behavioral intention toward organic food.
Greener and Whiter Analytical Chemistry Using Cyrene as a More Sustainable and Eco-Friendlier Mobile Phase Solvent in Chromatography
Authors: El Deeb Sami , Abdelsamad Khalid , Parr Maria Kristina
Published: August 2023

Cyrene was evaluated for the first time as a potential sustainable mobile phase solvent in re-versed-phase chromatography. As a benign biodegradable solvent, Cyrene is an attractive re-placement to classical non-green organic chromatographic solvents such as acetonitrile and a modifier, co-eluent to known green solvents such as ethanol. Compared to ethanol, Cyrene is less toxic, non-flammable, bio-based, biodegradable and cheaper solvent. A fire safety spider Chart was generated to compare the properties of Cyrene to ethanol and show its superiority as a greener solvent. Cyrene's behaviour, advantages, and drawbacks in reversed-phase chromatography, including cut-off value, elution power, selectivity, and effect on the column, were investigated using a model drug mixture of moxifloxacin and metronidazole. A monolithic C18 (10 x 4.6 mm) column was used as a stationary phase. Different ratios of Cyrene: ethanol with an aqueous portion of sodium acetate buffer mobile phases were tested. A mobile phase consisting of Cyrene: ethanol: 0.1 M sodium acetate buffer pH 4.25 (8:13:79, v/v) was selected as most suitable mobile phase system for separating and simultaneously determining metronidazole and moxifloxacin. The greenness and whiteness of the method were evaluated using the qualitative green assessment tool AGREE and the white analytical chemistry assessment tool RGB12. Further potentials of Cyrene as a solvent or modifier in normal phase chromatography, liquid chromatography-mass spectrometry, and supercritical fluid chromatography are discussed.
Immune Responses of Anti-HCV Antibody after Direct-Acting Antiviral Therapy Associated Allograft Injury with Acute Jaundice in Patients underwent Liver Transplantation
Authors: Lin Shu-Hsien , Wu Kun-Ta , Wang Chih-Chi , Huang Kuang-Tzu , Hsu Li-Wen , Eng Hock-Liew , Chiu King-Wah
Published: August 2023

Background and Aims: The impact of antibody response after direct-acting antiviral (DAA) therapy in hepatitis C virus (HCV)-infected recipients before and after liver transplantation (LT) is still undetermined. Methods: In this observational cohort study, we aimed to explore the association of changes in anti-HCV antibody titers after pre-LT DAA therapy with allograft injury, including biliary complications (BCs) and acute cellular rejection (ACR). Results: A total of 153 cases were enrolled from January 2015 to February 2021. Serum anti-HCV antibody titers were assessed before and after (day 30) LT. Among all recipients, 31/153 (20.3%) had pre-LT DAA therapy (DAA group) and 122/153 (79.7%) did not undergo pre-LT DAA therapy (DAA-naïve group). Higher incidence of post-LT BCs was observed in the DAA group (P = 0.028). Compared with the DAA-naïve group, the DAA group had a significantly higher mean level of anti-HCV titer upregulation (P = 0.0024); furthermore, among the recipients with BCs (n = 28) and ACR (n = 41), those in the DAA group exhibited significantly higher mean levels of anti-HCV antibody titer upregulation (P < 0.005). Conclusion: In conclusion, we speculated that anti-HCV antibody titer upregulation, which might have been induced by restoration of HCV-specific immune responses with pre-LT DAA therapy, were associated with post-LT allograft injury.
Trypanosoma cruzi STIB980: A TcI Strain for Drug Discovery and Reverse Genetics
Authors: Fesser Anna , Beilstein Sabina , Kaiser Marcel , Schmidt Remo S. , Mäser Pascal
Published: August 2023

Since the first published genome sequence of Trypanosoma cruzi in 2005, there has been tremendous technological advance in genomics, reverse genetics, and assay development for this elusive pathogen. However, there is still an unmet need for new and better drugs to treat Chagas disease. Here we introduce a T. cruzi assay strain that is useful for drug research as well as basic studies in host-pathogen interaction. Trypanosoma cruzi STIB980 is a strain of discrete typing unit TcI that grows well in culture as axenic epimastigotes or intracellular amastigotes. We have evaluated the optimal parameters for genetic transfection and constructed derivatives of T. cruzi STIB980 that express reporter genes for fluorescence- or bioluminescence-based drug efficacy testing, as well as a Cas9-expressing line for CRISPR/Cas9-mediated gene editing. The genome of T. cruzi STIB980 was sequenced by combining short-read Illumina with long-read Oxford Nanopore technologies. The latter served for the primary assembly, the former to correct mistakes and fill the gaps. This resulted in a high-quality nuclear haplotype assembly of 28 Mb in 400 contigs, containing 10,043 open-reading frames with a median length of 1077 bp. We believe that T. cruzi STIB980 is a useful addition to the antichagasic toolbox, and propose that it can serve as a DTU TcI reference strain for drug efficacy testing.
Association of Growth Hormone Gen with KUB Chicken Productivity
Authors: Alfano Prayoga , Depison Depison , Erina Silvia
Published: August 2023

This study aims to investigate the diversity of quantitative characteristics and GH genes, along with the association between GH genes and quantitative characteristics. The research material consisted of 96 KUB chickens aged DOC-2 months and corresponding blood samples. The t-test was utilized to determine differences in body weight, weight gain, and body measurements between male and female KUB chickens, and assess the diversity of the GH gene. T2-Hotelling analysis was employed to compare body measurements between male and female KUB chickens, while principal component analysis was used to identify size and shape characteristics. Male KUB chickens' average body weight, weight gain and body size were significantly (p<0.05) higher than females. The analysis of the growth hormone gene exhibited three genotypes: +/+ (0.51), +/- (0.35), and -/- (0.14). Additionally, two alleles were identified: (+) accounting for 0.68% and (-) accounting for 0.32%. The growth hormone gene MspI of KUB chickens demonstrated polymorphism, with X2 count (2.93) < X2 table 0.05 (3.84). The heterozygosity value in the KUB chicken population was 0.43, and the obtained PIC value was 0.38. The genotype +/+ of the growth hormone gene in KUB chickens was significantly higher (p<0.05) than genotypes +/- and -/-.In conclusion, male KUB chickens exhibited higher body weight, weight gain, and body measurements compared to female KUB chickens. The chest circumference served as the identifier for body size in both male and female KUB chickens, while the length of the upper body and tibia length distinguished the shape characteristics of male and female KUB chickens, respectively. KUB chickens' growth hormone gene MspI was associated with body weight, weight gain, and body measurements, with the (+/+) genotype being the most favorable
The Capability of Rice Plant Waste Sourced from the Feed Concentration Index in Farmer Groups in Salo Urban Vilagge
Authors: Surya Surya , Rahman Rahmaniar , Fitriawaty Fitriawaty , Andriani Ida , Amir Azhar
Published: August 2023

This study aims to determine the characteristics of farmer groups, rice plant waste production, livestock performance, livestock economic density and feed concentration index in Salo Urban Village. This study uses a descriptive cross-sectional research design. Data were analyzed to look at the characteristics of farmer groups according to sex and age, rice plant waste production, livestock performance, livestock economic density and feed concentration index in Salo Urban Village. The results showed 23 farmer groups with 1.077 members, 937 male (87%) and 140 female (13%). There were 2 categories: the productive category for those aged 15 - 64 years old (85,24%) and the unproductive category for those over 65 years old (14,76%). The average harvested area is 39,18 Ha/farmer group; with fresh production of rice plant waste being 465,80 tons; dry production of rice plant waste being 263,65 tons; and dry matter production being 233,49 tons. The livestock performance equation is In (Y) = -348.47 + 0.18t with a growth rate of 18% for cattle and In (Y) = -340.38 + 0.17t with a growth rate of 17% for goats, and has an economic density of livestock in the rarely category. The feed concentration index obtained three categories, namely the high production category (1 farmer group), the medium production category (6 farmer groups) and the low production category (16 farmer groups). Shows that rice plant waste harvested from farmer groups in Salo Urban Village can be used as a ruminant feed source.
The Effect of Surgeon Volume on Revision for Periprosthetic Joint Infection
Authors: Vaotuua D. , O’Connor P. , Belford M. , Lewis P. , Hatton A. , Holder C. , McAuliffe M.
Published: August 2023

Background:

Periprosthetic joint infection (PJI) is a devastating complication of total knee arthroplasty (TKA). An association between low surgeon volume and higher rates of infection following primary TKA has been suggested. The purpose of the present study was to determine if there was a relationship between surgeon volume and the rate of revision for infection after primary TKA.

Methods:

We searched the Australian Orthopaedic Association National Joint Replacement Registry (AOANJRR) to identify all primary TKA procedures that were performed for the treatment of osteoarthritis from September 1, 1999, to December 31, 2020, and were subsequently revised because of infection. Surgeon volume was defined as the annual volume of procedures performed by a surgeon during the same year in which the primary TKA (which was subsequently revised for infection) was performed. Surgeon volume was defined as <25, 25 to 49, 50 to 74, 75 to 99, or ≥100 primary TKA procedures/year. The cumulative percent revision (CPR) for infection was determined with use of Kaplan-Meier estimates. Cox proportional hazards methods were used to compare rates of revision for infection by surgeon volume, with subanalyses for patellar resurfacing and polyethylene use. Further analyses for patients <65 years of age and male patients were undertaken.

Results:

Overall, 602,919 primary TKA procedures were performed for the treatment of osteoarthritis, of which 5,295 were revised because of infection. High-volume surgeons (≥100 TKAs/year) had a significantly lower rate of revision for infection, with a CPR at 1 and 19 years of 0.4% (95% confidence interval [CI], 0.3 to 0.4) and 1.5% (95% CI, 1.2 to 2.0), respectively, compared with 0.6% (95% CI, 0.5 to 0.7) and 2.1% (95% CI, 1.8 to 2.3), respectively, for low-volume surgeons (<25 TKAs/year). Hazard ratios (HRs), adjusted for age and sex, comparing these 2 groups varied, depending on the time point, between 3.07 (95% CI, 2.02 to 4.68) and 1.44 (95% CI, 1.26 to 1.63) but remained significant (p < 0.001). When the analysis was adjusted for age, sex, American Society of Anesthesiologists (ASA) classification, and body mass index (BMI), there remained an increased risk of revision for PJI for all lower surgeon volume levels in comparison with the high- surgeon-volume group (≥100 TKAs/year). The results were similar when stratified by patellar resurfacing and cross-linked polyethylene (XLPE) and adjusted for age and sex.

Conclusions:

High-volume surgeons had lower rates of revision for infection. A better understanding of how surgical volume contributes to decreasing this complication is important and requires in-depth study.

Level of Evidence:

Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.