bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatio-Temporal Patterns of CO2 Emissions and Influencing Factors in China Using ESDA and PLS-SEM
Authors: Wang Bin , Zheng Qiuxia , Sun Ao , Bao Jie , Wu Dianting
Published: October 2021

Controlling carbon dioxide (CO2) emissions is the foundation of China’s goals to reach its carbon peak by 2030 and carbon neutrality by 2060. This study aimed to explore the spatial and temporal patterns and driving factors of CO2 emissions in China. First, we constructed a conceptual model of the factors influencing CO2 emissions, including economic growth, industrial structure, energy consumption, urban development, foreign trade, and government management. Second, we selected 30 provinces in China from 2006 to 2019 as research objects and adopted exploratory spatial data analysis (ESDA) methods to analyse the spatio-temporal patterns and agglomeration characteristics of CO2 emissions. Third, on the basis of 420 data samples from China, we used partial least squares structural equation modelling (PLS-SEM) to verify the validity of the conceptual model, analyse the reliability and validity of the measurement model, calculate the path coefficient, test the hypothesis, and estimate the predictive power of the structural model. Fourth, multigroup analysis (MGA) was used to compare differences in the influencing factors for CO2 emissions during different periods and in various regions of China. The results and conclusions are as follows: (1) CO2 emissions in China increased year by year from 2006 to 2019 but gradually decreased in the eastern, central, and western regions. The eastern coastal provinces show spatial agglomeration and CO2 emission hotspots. (2) Confirmatory analysis showed that the measurement model had high reliability and validity; four latent variables (industrial structure, energy consumption, economic growth, and government management) passed the hypothesis test in the structural model and are the determinants of CO2 emissions in China. Meanwhile, economic growth is a mediating variable of industrial structure, energy consumption, foreign trade, and government administration on CO2 emissions. (3) The calculated results of the R2 and Q2 values were 76.3% and 75.4%, respectively, indicating that the structural equation model had substantial explanatory and high predictive power. (4) Taking two development stages and three main regions as control groups, we found significant differences between the paths affecting CO2 emissions, which is consistent with China’s actual development and regional economic pattern. This study provides policy suggestions for CO2 emission reduction and sustainable development in China.
A Review on Ion-Exchange Membranes Fouling during Electrodialysis Process in Food Industry, Part 2: Influence on Transport Properties and Electrochemical Characteristics, Cleaning and Its Consequences
Authors: Pismenskaya Natalia , Bdiri Myriam , Sarapulova Veronika , Kozmai Anton , Fouilloux Julie , Baklouti Lassaad , Larchet Christian , Renard Estelle , Dammak Lasâad
Published: October 2021

Ion-exchange membranes (IEMs) are increasingly used in dialysis and electrodialysis processes for the extraction, fractionation and concentration of valuable components, as well as reagent-free control of liquid media pH in the food industry. Fouling of IEMs is specific compared to that observed in the case of reverse or direct osmosis, ultrafiltration, microfiltration, and other membrane processes. This specificity is determined by the high concentration of fixed groups in IEMs, as well as by the phenomena inherent only in electromembrane processes, i.e., induced by an electric field. This review analyzes modern scientific publications on the effect of foulants (mainly typical for the dairy, wine and fruit juice industries) on the structural, transport, mass transfer, and electrochemical characteristics of cation-exchange and anion-exchange membranes. The relationship between the nature of the foulant and the structure, physicochemical, transport properties and behavior of ion-exchange membranes in an electric field is analyzed using experimental data (ion exchange capacity, water content, conductivity, diffusion permeability, limiting current density, water splitting, electroconvection, etc.) and modern mathematical models. The implications of traditional chemical cleaning are taken into account in this analysis and modern non-destructive membrane cleaning methods are discussed. Finally, challenges for the near future were identified.
Cascading Effects of Root Microbial Symbiosis on the Development and Metabolome of the Insect Herbivore Manduca sexta L.
Authors: Papantoniou Dimitra , Vergara Fredd , Weinhold Alexander , Quijano Teresa , Khakimov Bekzod , Pattison David I. , Bak Søren , van Dam Nicole M. , Martínez-Medina Ainhoa
Published: October 2021

Root mutualistic microbes can modulate the production of plant secondary metabolites affecting plant–herbivore interactions. Still, the main mechanisms underlying the impact of root mutualists on herbivore performance remain ambiguous. In particular, little is known about how changes in the plant metabolome induced by root mutualists affect the insect metabolome and post-larval development. By using bioassays with tomato plants (Solanum lycopersicum), we analyzed the impact of the arbuscular mycorrhizal fungus Rhizophagus irregularis and the growth-promoting fungus Trichoderma harzianum on the plant interaction with the specialist insect herbivore Manduca sexta. We found that root colonization by the mutualistic microbes impaired insect development, including metamorphosis. By using untargeted metabolomics, we found that root colonization by the mutualistic microbes altered the secondary metabolism of tomato shoots, leading to enhanced levels of steroidal glycoalkaloids. Untargeted metabolomics further revealed that root colonization by the mutualists affected the metabolome of the herbivore, leading to an enhanced accumulation of steroidal glycoalkaloids and altered patterns of fatty acid amides and carnitine-derived metabolites. Our results indicate that the changes in the shoot metabolome triggered by root mutualistic microbes can cascade up altering the metabolome of the insects feeding on the colonized plants, thus affecting the insect development.
Archaeometric Analysis of the Objects from the Scala Santa (Holy Stairs) in the Crypt under the Piarist Church in Cracow (Poland)
Authors: Marszałek Mariola , Gaweł Adam , Pachuta Karolina , Buszko Eliza
Published: October 2021

Conservators extracted and preserved reliquaries hidden in the steps of the right flight of the Holy Stairs erected in the Piarist church crypt in Cracow (Poland). Three items from among 59 reliquaries were selected for specialist analyses: a framed, transparent cross containing a particle of the True Cross, and two opaque beads; an ornamented blue one without a hole and a drilled black one were analysed using non-destructive and non-invasive methods. The methods included scanning electron microscopy coupled with energy dispersive X-ray spectrometry, Raman microspectroscopy and X-ray diffractometry. The reliquary cross was found to be made of rock crystal and framed with an alloy of gold, silver and probably copper. The beads are made of glass; the blue bead represents forest plant-ash potash–lime glass and the black one, plant-ash soda–lime glass. Cobalt, probably along with copper, was used to produce the colour of the blue bead; manganese and iron ions were used to produce that of the black bead. Lead was present in both beads as one of the minor components and also as a component of corrosion products on their surfaces and probably also as part of the filler for the ornamentation of the blue bead. Nevertheless, it cannot be ruled out that the lead compounds were introduced intentionally to emphasize the bead ornamentation. The possible place and date of manufacture of the artefacts were also discussed.
In Search of the Perfect Triple BB Bond: Mechanical Tuning of the Host Molecular Trap for the Triple Bond B≡B Fragment
Authors: Zilberg Shmuel , Zinigrad Michael
Published: October 2021

The coordination of the B2 fragment by two σ-donor ligands L: could lead to a diboryne compound with a formal triple bond L:→B≡B←:L. σ-Type coordination L:→B leads to an excess of electrons around the B2 central fragment, whereas π-back-donation from the B≡B moiety to ligand L has a compensation effect. Coordination of the σ-donor and π-acceptor ligand is accompanied by the lowering of the BB bond order. Here, we propose a new approach to obtain the perfect triple BB bond through the incorporation of the BB unit into a rigid molecular capsule. The idea is the replacement of π-back-donation, as the principal stabilization factor in the linear NBBN structure, with the mechanical stabilization of the BB fragment in the inert molecular capsule, thus preserving the perfect B≡B triple bond. Quantum-chemical calculations show that the rigid molecular capsule provided a linear NBBN structure and an unusually short BB bond of 1.36 Å. Quantum-chemical calculations of the proposed diboryne adducts show a perfect triple bond B≡B without π-back-donation from the B2 unit to the host molecule. Two mechanisms were tested for the molecular design of a diboryne adduct with a perfect B≡B triple bond: the elimination of π-back-donation and the construction of a suitable molecular trap for the encapsulation of the B2 unit. The second factor that could lead to the strengthening or stretching of a selected chemical bond is molecular strain produced by the rigid molecular host capsule, as was shown for B≡B and for C≡C triple bonds. Different derivatives of icosane host molecules exhibited variation in BB bond length and the corresponding frequency of the BB stretch. On the other hand, this group of molecules shows a perfect triple BB bond character and they all possess a similar level of HOMO.
Antiviral Potential of Naphthoquinones Derivatives Encapsulated within Liposomes
Authors: Giongo Viveca , Falanga Annarita , De Melo Camilly P. Pires , da Silva Gustavo B. , Bellavita Rosa , De-Simone Salvatore G. , Paixão Izabel C. , Galdiero Stefania
Published: October 2021

HSV infections, both type 1 and type 2, are among the most widespread viral diseases affecting people of all ages. Their symptoms could be mild, with cold sores up to 10 days of infection, blindness and encephalitis caused by HSV-1 affecting immunocompetent and immunosuppressed individuals. The severe effects derive from co-evolution with the host, resulting in immune evasion mechanisms, including latency and growing resistance to acyclovir and derivatives. An efficient alternative to controlling the spreading of HSV mutations is the exploitation of new drugs, and the possibility of enhancing their delivery through the encapsulation of drugs into nanoparticles, such as liposomes. In this work, liposomes were loaded with a series of 2-aminomethyl- 3-hydroxy-1,4-naphthoquinones derivatives with n-butyl (compound 1), benzyl (compound 2) and nitrobenzene (compound 3) substituents in the primary amine of naphthoquinone. They were previously identified to have significant inhibitory activity against HSV-1. All of the aminomethylnaphthoquinones derivatives encapsulated in the phosphatidylcholine liposomes were able to control the early and late phases of HSV-1 replication, especially those substituted with the benzyl (compound 2) and nitrobenzene (compound 3), which yields selective index values that are almost nine times more efficient than acyclovir. The growing interest of the industry in topical administration against HSV supports our choice of liposome as a drug carrier of aminomethylnaphthoquinones derivatives for formulations of in vivo pre-clinical assays.
CO2 Adsorption on Modified Mesoporous Silicas: The Role of the Adsorption Sites
Authors: Ravutsov Martin , Mitrev Yavor , Shestakova Pavletta , Lazarova Hristina , Simeonov Svilen , Popova Margarita
Published: October 2021

The post-synthesis procedure for cyclic amine (morpholine and 1-methylpiperazine) modified mesoporous MCM-48 and SBA-15 silicas was developed. The procedure for preparation of the modified mesoporous materials does not affect the structural characteristics of the initial mesoporous silicas strongly. The initial and modified materials were characterized by XRD, N2 physisorption, thermal analysis, and solid-state NMR. The CO2 adsorption of the obtained materials was tested under dynamic and equilibrium conditions. The NMR data revealed the formation of different CO2 adsorbed forms. The materials exhibited high CO2 absorption capacity lying above the benchmark value of 2 mmol/g and stretching out to the outstanding 4.4 mmol/g in the case of 1-methylpiperazin modified MCM-48. The materials are reusable, and their CO2 adsorption capacities are slightly lower in three adsorption/desorption cycles.
Graphene Transfer: A Physical Perspective
Authors: Langston Xavier , Whitener Keith E.
Published: October 2021

Graphene, synthesized either epitaxially on silicon carbide or via chemical vapor deposition (CVD) on a transition metal, is gathering an increasing amount of interest from industrial and commercial ventures due to its remarkable electronic, mechanical, and thermal properties, as well as the ease with which it can be incorporated into devices. To exploit these superlative properties, it is generally necessary to transfer graphene from its conductive growth substrate to a more appropriate target substrate. In this review, we analyze the literature describing graphene transfer methods developed over the last decade. We present a simple physical model of the adhesion of graphene to its substrate, and we use this model to organize the various graphene transfer techniques by how they tackle the problem of modulating the adhesion energy between graphene and its substrate. We consider the challenges inherent in both delamination of graphene from its original substrate as well as relamination of graphene onto its target substrate, and we show how our simple model can rationalize various transfer strategies to mitigate these challenges and overcome the introduction of impurities and defects into the graphene. Our analysis of graphene transfer strategies concludes with a suggestion of possible future directions for the field.
Amino Acid Profile in Malnourished Patients with Liver Cirrhosis and Its Modification with Oral Nutritional Supplements: Implications on Minimal Hepatic Encephalopathy
Authors: Espina Silvia , Gonzalez-Irazabal Yolanda , Sanz-Paris Alejandro , Lopez-Yus Marta , Garcia-Sobreviela Maria Pilar , del Moral-Bergos Raquel , Garcia-Rodriguez Beatriz , Fuentes-Olmo Javier , Bernal-Monterde Vanesa , Arbones-Mainar Jose M.
Published: October 2021

Low plasma levels of branched chain amino acids (BCAA) in liver cirrhosis are associated with hepatic encephalopathy (HE). We aimed to identify a metabolic signature of minimal hepatic encephalopathy (MHE) in malnourished cirrhotic patients and evaluate its modification with oral nutritional supplements (ONS) enriched with ß-Hydroxy-ß-methylbutyrate (HMB), a derivative of the BCAA leucine. Post hoc analysis was conducted on a double-blind placebo-controlled trial of 43 individuals with cirrhosis and malnutrition, who were randomized to receive, for 12 weeks, oral supplementation twice a day with either 220 mL of Ensure® Plus Advance (HMB group, n = 22) or with 220 mL of Ensure® Plus High Protein (HP group, n = 21). MHE evaluation was by psychometric hepatic encephalopathy score (PHES). Compared to the HP group, an HMB-specific treatment effect led to a larger increase in Val, Leu, Phe, Trp and BCAA fasting plasma levels. Both treatments increased Fischer’s ratio and urea without an increase in Gln or ammonia fasting plasma levels. MHE was associated with a reduced total plasma amino acid concentration, a reduced BCAA and Fischer´s ratio, and an increased Gln/Glu ratio. HMB-enriched ONS increased Fischer´s ratio without varying Gln or ammonia plasma levels in liver cirrhosis and malnutrition, a protective amino acid profile that can help prevent MHE.
Designing Lifestyle Interventions for Common Mental Disorders: What Can We Learn from Diabetes Prevention Programs?
Authors: Opie Rachelle S , Jacka Felice N , Marx Wolfgang , Rocks Tetyana , Young Claire , O’Neil Adrienne
Published: October 2021

Lifestyle factors including diet, sleep, physical activity, and substance use cessation, are recognised as treatment targets for common mental disorders (CMDs). As the field of lifestyle-based mental health care evolves towards effectiveness trials and real-world translation, it is timely to consider how such innovations can be integrated into clinical practice. This paper discusses the utility and scale-up of lifestyle interventions for CMDs and draws on diabetes prevention literature to identify enablers and barriers to translation efforts. We discuss the extent to which lifestyle interventions aimed at managing CMDs and preventing diabetes share commonalities (program content, theoretical underpinnings, program structures, interventionists, frameworks promoting fidelity, quality, sustainability). Specific considerations when utilising these programs for mental health include personalising content with respect to symptoms and trajectories of depression and anxiety, medication regimen and genetic risk profile. As this field moves from efficacy to effectiveness and implementation, it is important to ensure issues in implementation science, including “voltage drop”, “program drift”, logistics, funding, and resourcing, are in line with evidence-based models that are effective in research settings. Ongoing considerations includes who is best placed to deliver this care and the need for models to support implementation including long-term financing, workforce training, supervision, stakeholder and organisational support.
Association between Parents’ Perceptions of Preschool Children’s Weight, Feeding Practices and Children’s Dietary Patterns: A Cross-Sectional Study in China
Authors: Xiang Caihong , Zhang Youjie , Yong Cuiting , Xi Yue , Huo Jiaqi , Zou Hanshuang , Liang Jiajing , Jiang Zhiqian , Lin Qian
Published: October 2021

Parental perception of children’s weight may influence parents’ feeding practices, and in turn, child dietary intake and weight status; however, there is limited evidence generated for preschoolers. The aim of this cross-sectional study was to investigate associations between Chinese parents’ perceptions of child weight, feeding practices and preschoolers’ dietary patterns. Participants (1616 parent-child pairs) were recruited from six kindergartens in Hunan, China. Parents’ misperception, concern, and dissatisfaction on child weight were collected through a self-administered caregiver questionnaire. Parental feeding practices and children’s dietary intake were, respectively, assessed using the Child Feeding Questionnaire and a Food Frequency Questionnaire. Linear regression models were applied to analyze associations between parental weight perceptions, feeding practices, and preschooler’s dietary patterns. Associations between parents’ weight perceptions and dietary patterns were significant only among underweight children. Regardless of child weight status, parental weight underestimation and preference for a heavier child were positively associated with pressure-to-eat. Parental weight concern was positively associated with restriction in normal weight child, but this was not found in other weight groups. In conclusion, Parents’ misperception, concern, and dissatisfaction about child weight are associated with parents’ feeding practices and may influence preschoolers’ dietary quality, but the relationships vary by children’s actual weight status.
Mediterranean Diet, Psychological Adjustment and Health Perception in University Students: The Mediating Effect of Healthy and Unhealthy Food Groups
Authors: Vélez-Toral Mercedes , Morales-Domínguez Zaira , Granado-Alcón María del Carmen , Díaz-Milanés Diego , Andrés-Villas Montserrat
Published: October 2021

This study aims to identify the relationships between eating habits and psychological adjustment and health perception, and to analyze potential mediating role of healthy and unhealthy foods in the relationship between adherence to the Mediterranean diet (MedDiet) and the psychological constructs and health perception. The sample was selected through stratified random cluster sampling and was composed of 788 university students. The participants responded to a MedDiet adherence screener and food consumption inventory to assess the eating habits, instruments measuring self-esteem, life satisfaction, curiosity and sense of coherence to assess the psychological adjustment, and single item measuring perceived health. The results revealed 41.9% of the participants had a high consumption of vegetables and 85.1% a low consumption of energy drinks, while 29.9% showed a high adherence to the MedDiet which was positively associated to each psychological variable and healthy foods and negatively with unhealthy foods. In conclusion, a higher adherence to the MedDiet, and the consumption of fruits and vegetables is related to higher psychological adjustment and health perception. However, the relationships between MedDiet and the psychological variables and health perception were fully or partially explained because of the consumption of healthy and unhealthy foods.
An Evaluation of Omega-3 Status and Intake in Canadian Elite Rugby 7s Players
Authors: Armstrong Ashley , Anzalone Anthony J. , Pethick Wendy , Murray Holly , Dahlquist Dylan T. , Askow Andrew T. , Heileson Jeffery L. , Hillyer Lyn M. , Ma David W. L. , Oliver Jonathan M.
Published: October 2021

Background: EPA and DHA n-3 FA play crucial roles in both neurological and cardiovascular health and high dietary intakes along with supplementation suggest potential neuroprotection and concussion recovery support. Rugby athletes have a high risk of repetitive sub-concussive head impacts which may lead to long-term neurological deficits, but there is a lack of research looking into n-3 FA status in rugby players. We examined the dietary n-3 FA intake through a FFQ and n-3 FA status by measuring the percentage of n-3 FA and O3I in elite Canadian Rugby 7s players to show distribution across O3I risk zones; high risk, <4%; intermediate risk, 4 to 8%; and low risk, >8%. Methods: n-3 FA profile and dietary intake as per FFQ were collected at the beginning of the 2017–2018 Rugby 7s season in male (n = 19; 24.84 ± 2.32 years; 95.23 ± 6.93 kg) and female (n = 15; 23.45 ± 3.10 years; 71.21 ± 5.79 kg) athletes. Results: O3I averaged 4.54% ± 1.77, with female athlete scores slightly higher, and higher O3I scores in supplemented athletes (4.82% vs. 3.94%, p = 0.183), with a greater proportion of non-supplemented athletes in the high-risk category (45.5% vs. 39.1%). Dietary intake in non-supplemented athletes did not meet daily dietary recommendations for ALA or EPA + DHA compared to supplemented athletes. Conclusions: Overall, despite supplementation, O3I score remained in the high-risk category in a proportion of athletes who met recommended n-3 FA dietary intakes, and non-supplemented athletes had a higher proportion of O3I scores in the high-risk category, suggesting that dietary intake alone may not be enough and athletes may require additional dietary and n-3 FA supplementation to reduce neurological and cardiovascular risk.
Health-Related Lifestyle Profiles in Healthy Adults: Associations with Sociodemographic Indicators, Dispositional Optimism, and Sense of Coherence
Authors: Adorni Roberta , Zanatta Francesco , D’Addario Marco , Atella Francesca , Costantino Elena , Iaderosa Caterina , Petarle Giulia , Steca Patrizia
Published: October 2021

Cardiovascular disease (CVD) is the leading cause of morbidity and mortality globally. Promoting healthy behaviors throughout life is an essential prevention tool. This study investigated the associations among lifestyle profiles (including diet, alcohol consumption, physical activity, cigarette smoking, and cardiovascular screening), sociodemographic factors (gender, age, education, and family history of CVDs), and psychological factors (sense of coherence and dispositional optimism). In total, 676 healthy adults (mean age = 35 years; range = 19–57; 46% male) participated in an online survey. Lifestyle profiles were identified through cluster analysis, and a multinomial logistic regression was then performed to explore their association with sociodemographic and psychological variables. Results show that men were more likely than women to belong to the lifestyle profile with the highest amount of physical activity (OR = 2.40; p < 0.001) and the greatest attention to cardiovascular screening (OR = 2.09; p < 0.01). Lower dispositional optimism was associated with the profile paying the greatest attention to cardiovascular screening (OR = 0.67; p < 0.05). Sense of coherence, in terms of lower comprehensibility (OR = 0.67; p < 0.05) and higher manageability (OR = 1.43; p < 0.05), was associated with the lifestyle profile characterized by an unhealthy diet, sedentary lifestyle, and nonsmoking. This study shed light on factors associated with different co-occurring health-related behaviors that should be considered in planning effective communication strategies promoting adherence to health claims.
Proportions of Polyunsaturated Fatty Acids in Umbilical Cord Blood at Birth Are Related to Atopic Eczema Development in the First Year of Life
Authors: Barman Malin , Stråvik Mia , Broberg Karin , Sandin Anna , Wold Agnes E. , Sandberg Ann-Sofie
Published: October 2021

Atopic eczema, the most common atopic disease in infants, may pave the way for sensitization and allergy later in childhood. Fatty acids have immune-regulating properties and may regulate skin permeability. Here we examine whether the proportions of fatty acids among the infant and maternal plasma phospholipids at birth were associated with maternal dietary intake during pregnancy and development of atopic eczema during the first year of age in the Nutritional impact on Immunological maturation during Childhood in relation to the Environment (NICE) birth cohort. Dietary data were collected with a semi-quantitative food frequency questionnaire, fatty acids were measured with GC-MS and atopic eczema was diagnosed by a pediatric allergologist at 12 months of age. We found that higher proportions of n-6 PUFAs (including arachidonic acid) but lower proportions of n-3 PUFAs (including DPA) in the infant’s phospholipids at birth were associated with an increased risk of atopic eczema at 12 months of age. The n-6 and n-3 PUFAs were related to maternal intake of meat and fish, respectively. Our results suggest that prenatal exposure to unsaturated fatty acids is associated with eczema development in the infant. Maternal diet during pregnancy may partly explain the fatty acid profiles in utero.
Human Immunodeficiency Virus Type 2: The Neglected Threat
Authors: Ceccarelli Giancarlo , Giovanetti Marta , Sagnelli Caterina , Ciccozzi Alessandra , d’Ettorre Gabriella , Angeletti Silvia , Borsetti Alessandra , Ciccozzi Massimo
Published: October 2021

West Africa has the highest prevalence of human immunodeficiency virus (HIV)-2 infection in the world, but a high number of cases has been recognized in Europe, India, and the United States. The virus is less transmissible than HIV-1, with sexual contacts being the most frequent route of acquisition. In the absence of specific antiretroviral therapy, most HIV-2 carriers will develop AIDS. Although, it requires more time than HIV-1 infection, CD4+ T cell decline occurs more slowly in HIV-2 than in HIV-1 patients. HIV-2 is resistant to non-nucleoside reverse transcriptase inhibitors (NNRTIs) and some protease inhibitors. Misdiagnosis of HIV-2 in patients mistakenly considered HIV-1-positive or in those with dual infections can cause treatment failures with undetectable HIV-1 RNA. In this era of global integration, clinicians must be aware of when to consider the diagnosis of HIV-2 infection and how to test for this virus. Although there is debate regarding when therapy should be initiated and which regimen should be chosen, recent trials have provided important information on treatment options for HIV-2 infection. In this review, we focus mainly on data available and on the insight they offer about molecular epidemiology, clinical presentation, antiretroviral therapy, and diagnostic tests of HIV-2 infection.
Targeted Delivery of Epidermal Growth Factor to the Human Placenta to Treat Fetal Growth Restriction
Authors: Renshall Lewis J. , Beards Frances , Evangelinos Angelos , Greenwood Susan L. , Brownbill Paul , Stevens Adam , Sibley Colin P. , Aplin John D. , Johnstone Edward D. , Teesalu Tambet , Harris Lynda K.
Published: October 2021

Placental dysfunction is the underlying cause of pregnancy complications such as fetal growth restriction (FGR) and pre-eclampsia. No therapies are available to treat a poorly functioning placenta, primarily due to the risks of adverse side effects in both the mother and the fetus resulting from systemic drug delivery. The use of targeted liposomes to selectively deliver payloads to the placenta has the potential to overcome these issues. In this study, we assessed the safety and efficacy of epidermal growth factor (EGF)-loaded, peptide-decorated liposomes to improve different aspects of placental function, using tissue from healthy control pregnancies at term, and pregnancies complicated by FGR. Phage screening identified a peptide sequence, CGPSARAPC (GPS), which selectively homed to mouse placentas in vivo, and bound to the outer syncytiotrophoblast layer of human placental explants ex vivo. GPS-decorated liposomes were prepared containing PBS or EGF (50–100 ng/mL), and placental explants were cultured with liposomes for up to 48 h. Undecorated and GPS-decorated liposomes containing PBS did not affect the basal rate of amino acid transport, human chorionic gonadotropin (hCG) release or cell turnover in placental explants from healthy controls. GPS-decorated liposomes containing EGF significantly increased amino acid transporter activity in healthy control explants, but not in placental explants from women with FGR. hCG secretion and cell turnover were unaffected by EGF delivery; however, differential activation of downstream protein kinases was observed when EGF was delivered via GPS-decorated vs. undecorated liposomes. These data indicate that targeted liposomes represent a safe and useful tool for the development of new therapies for placental dysfunction, recapitulating the effects of free EGF.
Hyaluronan-Cholesterol Nanogels for the Enhancement of the Ocular Delivery of Therapeutics
Authors: Zoratto Nicole , Forcina Laura , Matassa Roberto , Mosca Luciana , Familiari Giuseppe , Musarò Antonio , Mattei Maurizio , Coviello Tommasina , Di Meo Chiara , Matricardi Pietro
Published: October 2021

The anatomy and physiology of the eye strongly limit the bioavailability of locally administered drugs. The entrapment of therapeutics into nanocarriers represents an effective strategy for the topical treatment of several ocular disorders, as they may protect the embedded molecules, enabling drug residence on the ocular surface and/or its penetration into different ocular compartments. The present work shows the activity of hyaluronan-cholesterol nanogels (NHs) as ocular permeation enhancers. Thanks to their bioadhesive properties, NHs firmly interact with the superficial corneal epithelium, without penetrating the stroma, thus modifying the transcorneal penetration of loaded therapeutics. Ex vivo transcorneal permeation experiments show that the permeation of hydrophilic drugs (i.e., tobramycin and diclofenac sodium salt), loaded in NHs, is significantly enhanced when compared to the free drug solutions. On the other side, the permeation of hydrophobic drugs (i.e., dexamethasone and piroxicam) is strongly dependent on the water solubility of the entrapped molecules. The obtained results suggest that NHs formulations can improve the ocular bioavailability of the instilled drugs by increasing their preocular retention time (hydrophobic drugs) or facilitating their permeation (hydrophilic drugs), thus opening the route for the application of HA-based NHs in the treatment of both anterior and posterior eye segment diseases.
Trait Variations and Probability Grading Index System on Leaf-Related Traits of Eucommia ulmoides Oliver Germplasm
Authors: Deng Peng , Xie Xiangchen , Long Feiyu , Zhang Liang , Li Yonghang , Zhao Zhangxu , Yang Shiyao , Wang Yiran , Fan Ruishen , Li Zhouqi
Published: October 2021

Eucommia ulmoides Oliver (EUO), an economic tree grown specifically in China, is widely used in various fields. To satisfy the requirements of industrial development, superior varieties need to be selected for different uses. However, there is no unified standard for breeders to reference. In this study, leaf-related traits were classified by a probability grading method. The results indicated there were significant differences between different planting models for the studied traits, and the traits in the Arbor forest model showed more abundant variation. Compared with genotype, the planting model accounted for relatively bigger variance, indicating that the standard should be divided according to planting models. Furthermore, the optimum planting model for different traits would be obtained by analyzing the variation range. Association analyses were conducted among traits to select the crucial evaluation indexes. The indexes were divided into three grades in different planting models. The evaluation system on leaf-related traits of EUO germplasm was established preliminarily, which considered planting models and stability across years for the first time. It can be treated as a reference to identify and evaluate EUO germplasm resources. Additionally, the study served as an example for the classification of quantitative traits in other economically important perennial plants.
Aster glehni F. Schmidt Extract Modulates the Activities of HMG-CoA Reductase and Fatty Acid Synthase
Authors: Lee Hyunbeom , Kim Hyoung Ja , Chae Hyungi , Yoon Na Eun , Jung Byung Hwa
Published: October 2021

Aster glehni F. Schmidt (AG), is a natural product known to have anti-obesity effects, but the mechanism underlying these effects is not well documented. We hypothesized that AG may have inhibitory effects on enzymes related to lipid accumulation. Herein, AG fractions were tested against HMG-CoA reductase (HMGR) and fatty acid synthase (FAS), two important enzymes involved in cholesterol and fatty acid synthesis, respectively. We found that dicaffeoylquinic acid (DCQA) methyl esters present in AG are largely responsible for the inhibition of HMGR and FAS. Since free DCQA is a major form present in AG, we demonstrated that a simple methylation of the AG extract could increase the overall inhibitory effects against those enzymes. Through this simple process, we were able to increase the inhibitory effect by 150%. We believe that our processed AG effectively modulates the HMGR and FAS activities, providing promising therapeutic potential for cholesterol- and lipid-lowering effects.
Analysis of Chromosome Associations during Early Meiosis in Wheat Lines Carrying Chromosome Introgressions from Agropyron cristatum
Authors: Prieto Pilar , Palomino Carmen , Cifuentes Zuny , Cabrera Adoración
Published: October 2021

Crested wheatgrass (Agropyron cristatum L. Gaertn., genome P), included in the Triticeae tribe (family Poaceae), is one of the most important grasses in temperate regions. It has been valued as a donor of important agronomic traits for wheat improvement, including tolerance to cold, drought, and high salinity, as well as resistance to leaf rust, stripe rust, and powdery mildew. For successful incorporation of beneficial alleles into wheat, it is essential that recombination between wheat and A. cristatum chromosomes occurs. In this work, we analysed chromosome associations during meiosis in wheat lines carrying chromosome introgressions from A. cristatum chromosomes 5P and 6P in the presence and absence of Ph1 locus using fluorescence in situ hybridisation. The results showed that the Ph1 locus does not affect chromosome associations between A. cristatum and wheat chromosomes because there were no interspecific chromosome associations; therefore, no recombination between chromosomes from wheat and Agropyron were observed in the absence of the Ph1 locus. The 5P and 6P A. cristatum chromosomes do not have a suppressor effect on the Ph1 locus. Wheat univalents in metaphase I suggest that Agropyron chromosomes might carry genes having a role in wheat homologous chromosome associations. Putative effect of the Agropyron genes on wheat chromosome associations does not interact with the Ph1 locus.
First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain
Authors: Canales Celia , Morán Félix , Olmos Antonio , Ruiz-García Ana Belén
Published: October 2021

Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.
Structure of Polytetrafluoroethylene Modified by the Combined Action of γ-Radiation and High Temperatures
Authors: Smolyanskii Alexander Sergeevich , Politova Ekaterina Dmitrievna , Koshkina Ol’ga Alekseevna , Arsentyev Mikhail Aleksandrovich , Kusch Pavel Prokof’evich , Moskvitin Lev Vladimirovich , Slesarenko Sergei Vital’evich , Kiryukhin Dmitrii Pavlovich , Trakhtenberg Leonid Izrailevich
Published: October 2021

By means of X-ray computed microtomography (XCMT), the existence of a developed porous structure with an average pore diameter of ~3.5 μm and pore content of ~1.1 vol.% has been revealed in unirradiated polytetrafluoroethylene (PTFE). It has been found that the combined action of gamma radiation (absorbed dose per PTFE of ~170 kGy) and high temperatures (327–350 °C) leads to the disappearance of the porous structure and the formation of several large pores with sizes from 30 to 50 μm in the bulk of thermal-radiation modified PTFE (TRM-PTFE). It has been established by X-ray diffraction (XRD) analysis that the thermal-radiation modification of PTFE leads to an increase in the interplanar spacings, the degree of crystallinity and the volume of the unit cell, as well as to a decrease in the size of crystals and the X-ray density of the crystalline phase in comparison with the initial polymer. It is assumed that the previously-established effect of improving the deformation-strength and tribological properties of the TRM-PTFE can be due not only to the radiation cross-linking of polymer chains but also to the disappearance of the pore system and to the ordering of the crystalline phase of PTFE.
Reviewing the State-of-the-Art of Smart Cities in Portugal: Evidence Based on Content Analysis of a Portuguese Magazine
Authors: Correia Diogo , Teixeira Leonor , Marques João Lourenço
Published: October 2021

The lack of examples of smart-city initiatives and the sharing of best practices in Portugal confirm the gap in the transference of empirical knowledge to the scientific literature in this area. The smart-city concept has passed through three stages. However, its evolution has not been noted equally throughout countries and their territories. The literature only provides information about specific projects implemented in a few cities. Therefore, the aim of this paper was to study the state-of-the-art of smart cities in Portugal by analyzing 25 editions of the most relevant national-wide smart-cities magazine. First, the objective of analyzing the magazine was to study each Portuguese city in terms of the subject areas and types of existing initiatives in order, ultimately, to frame cities within their respective smart-city phases, as per the literature. Second, the aim of the paper was also to provide information about the evolution of the concept through analyses of embedded experts’ quotes. The results of the first are complemented with the analysis of interviews with policymakers to provide information about the existing challenges to implementing a smart city and to understand the role of government therein. Qualitative and quantitative analyses were performed on the case study. The findings suggest that the three smart-city phases are perceived in slightly different ways in Portugal and heterogeneity within the country can be noted from the lack of strategies and a standard framework.
Forest Vertical Structure Mapping Using Two-Seasonal Optic Images and LiDAR DSM Acquired from UAV Platform through Random Forest, XGBoost, and Support Vector Machine Approaches
Authors: Yu Jin-Woo , Yoon Young-Woong , Baek Won-Kyung , Jung Hyung-Sup
Published: October 2021

Research on the forest structure classification is essential, as it plays an important role in assessing the vitality and diversity of vegetation. However, classifying forest structure involves in situ surveying, which requires considerable time and money, and cannot be conducted directly in some instances; also, the update cycle of the classification data is very late. To overcome these drawbacks, feasibility studies on mapping the forest vertical structure from aerial images using machine learning techniques were conducted. In this study, we investigated (1) the performance improvement of the forest structure classification, using a high-resolution LiDAR-derived digital surface model (DSM) acquired from an unmanned aerial vehicle (UAV) platform and (2) the performance comparison of results obtained from the single-seasonal and two-seasonal data, using random forest (RF), extreme gradient boosting (XGBoost), and support vector machine (SVM). For the performance comparison, the UAV optic and LiDAR data were divided into three cases: (1) only used autumn data, (2) only used winter data, and (3) used both autumn and winter data. From the results, the best model was XGBoost, and the F1 scores achieved using this method were approximately 0.92 in the autumn and winter cases. A remarkable improvement was achieved when both two-seasonal images were used. The F1 score improved by 35.3% from 0.68 to 0.92. This implies that (1) the seasonal variation in the forest vertical structure can be more important than the spatial resolution, and (2) the classification performance achieved from the two-seasonal UAV optic images and LiDAR-derived DSMs can reach 0.9 with the application of an optimal machine learning approach.
Characteristics of Deep Convective Systems and Initiation during Warm Seasons over China and Its Vicinity
Authors: Li Yang , Liu Yubao , Chen Yun , Chen Baojun , Zhang Xin , Wang Weisheng , Shu Zhuozhi , Huo Zhaoyang
Published: October 2021

The spatiotemporal statistical characteristics of warm-season deep convective systems, particularly deep convective systems initiation (DCSI), over China and its vicinity are investigated using Himawari-8 geostationary satellite measurements collected during April-September from 2016 to 2020. Based on a satellite brightness temperature multiple-threshold convection identification and tracking method, a total of 47593 deep convective systems with lifetimes of at least 3 h were identified in the region. There are three outstanding local maxima in the region, located in the southwestern, central and eastern Tibetan Plateau and Yunnan-Guizhou Plateau, followed by a region of high convective activities in South China. Most convective systems are developed over the Tibetan Plateau, predominantly eastward-moving, while those developed in Yunnan-Guizhou Plateau and South China mostly move westward and southwestward. The DSCI occurrences become extremely active after the onset of the summer monsoon and tend to reach a maximum in July and August, with a diurnal peak at 11–13 LST in response to the enhanced solar heating and monsoon flows. Several DCSI hotspots are identified in the regions of inland mountains, tropical islands and coastal mountains during daytime, but in basins, plains and coastal areas during nighttime. DCSI over land and oceans exhibits significantly different sub-seasonal and diurnal variations. Oceanic DCSI has an ambiguous diurnal variation, although its sub-seasonal variation is similar to that over land. It is demonstrated that the high spatiotemporal resolution satellite dataset provides rich information for understanding the convective systems over China and vicinity, particularly the complex terrain and oceans where radar observations are sparse or none, which will help to improve the convective systems and initiation nowcasting.
Chip Appearance Defect Recognition Based on Convolutional Neural Network
Authors: Wang Jun , Zhou Xiaomeng , Wu Jingjing
Published: October 2021

To improve the recognition rate of chip appearance defects, an algorithm based on a convolution neural network is proposed to identify chip appearance defects of various shapes and features. Furthermore, to address the problems of long training time and low accuracy caused by redundant input samples, an automatic data sample cleaning algorithm based on prior knowledge is proposed to reduce training and classification time, as well as improve the recognition rate. First, defect positions are determined by performing image processing and region-of-interest extraction. Subsequently, interference samples between chip defects are analyzed for data cleaning. Finally, a chip appearance defect classification model based on a convolutional neural network is constructed. The experimental results show that the recognition miss detection rate of this algorithm is zero, and the accuracy rate exceeds 99.5%, thereby fulfilling industry requirements.
Unlocking the Potential of Fintechs for Financial Inclusion: A Delphi-Based Approach
Authors: Joia Luiz Antonio , Cordeiro Joaquim Pedro Vasconcelos
Published: October 2021

The financial sector is experiencing an accelerated process of transformation shaped by fintechs, which opens an important window of opportunity to increase financial inclusion in emerging markets, such as Brazil, with high financial exclusion. Thus, this article investigates, through a Delphi approach involving fintech professionals, the potential of fintechs to enable financial inclusion in emerging markets, using Brazil as a proxy. The analysis carried out identified three domains related to fintechs that have the potential to impact financial inclusion: (i) fintechs can serve niches of people without a bank account in the traditional financial market, (ii) fintechs can reduce costs for clients through increased competition, and (iii) fintechs can offer financial services in remote locations, far from traditional financial institutions. Thus, with the objective of developing a public agenda of financial inclusion through fintechs, the article proposes four lines of public policies: (i) expansion and modernization of mobile and internet infrastructure, (ii) improvement of the population’s financial and digital education, (iii) implementation of a trustworthy environment for the fintech clients, and (iv) development and enforcement of an effective legal and regulatory framework for fintechs. These policies, if implemented, can benefit people excluded from the financial system around the world.