bluuebunny/crossref_metadata_2025 huggingface dataset:
Automatic Sensory Predictions: A Review of Predictive Mechanisms in the Brain and Their Link to Conscious Processing
Authors: Tivadar Ruxandra I. , Knight Robert T. , Tzovara Athina
Published: August 2021

The human brain has the astonishing capacity of integrating streams of sensory information from the environment and forming predictions about future events in an automatic way. Despite being initially developed for visual processing, the bulk of predictive coding research has subsequently focused on auditory processing, with the famous mismatch negativity signal as possibly the most studied signature of a surprise or prediction error (PE) signal. Auditory PEs are present during various consciousness states. Intriguingly, their presence and characteristics have been linked with residual levels of consciousness and return of awareness. In this review we first give an overview of the neural substrates of predictive processes in the auditory modality and their relation to consciousness. Then, we focus on different states of consciousness - wakefulness, sleep, anesthesia, coma, meditation, and hypnosis - and on what mysteries predictive processing has been able to disclose about brain functioning in such states. We review studies investigating how the neural signatures of auditory predictions are modulated by states of reduced or lacking consciousness. As a future outlook, we propose the combination of electrophysiological and computational techniques that will allow investigation of which facets of sensory predictive processes are maintained when consciousness fades away.
Providing Measurement, Evaluation, Accountability, and Leadership Support (MEALS) for Non-communicable Diseases Prevention in Ghana: Project Implementation Protocol
Authors: Laar Amos , Kelly Bridget , Holdsworth Michelle , Quarpong Wilhemina , Aryeetey Richmond , Amevinya Gideon Senyo , Tandoh Akua , Agyemang Charles , Zotor Francis , Laar Matilda E. , Mensah Kobby , Laryea Dennis , Asiki Gershim , Pradeilles Rebecca , Sellen Daniel , L'Abbe Mary R. , Vandevijvere Stefanie
Published: August 2021

Background: This study describes the rationale, adaptation, and final protocol of a project developed to address the increase in obesity and nutrition-related non-communicable diseases (NR-NCDs) in Ghana. Code-named the Measurement, Evaluation, Accountability, and Leadership Support for NCDs (MEALS4NCDs) project, it aims to measure and support public sector actions that create healthy food marketing, retail, and provisioning environments for Ghanaian children using adapted methods from the International Network for Food and Obesity/NCDs Research Monitoring and Action Support (INFORMAS).

Methods: The protocol for this observational study draws substantially from the INFORMAS' Food Promotion and Food Provision Modules. However, to appraise the readiness of local communities to implement interventions with strong potential to improve food environments of Ghanaian children, the MEALS4NCDs protocol has innovatively integrated a local community participatory approach based on the community readiness model (CRM) into the INFORMAS approaches. The setting is Ghana, and the participants include health and nutrition policy-makers, nutrition and food service providers, consumers, school authorities, and pupils of Ghanaian basic schools.

Results: The study establishes a standardized approach to providing implementation science evidence for the prevention of non-communicable diseases (NCDs) in Ghana. It demonstrates feasibility and the innovative application of the INFORMAS expanded food promotion and food provision modules, together with the integration of the CRM in a lower-middle income setting.

Conclusion: The research will facilitate the understanding of the processes through which the INFORMAS approach is contextualized to a lower-middle income African context. The protocol could be adapted for similar country settings to monitor relevant aspects of food environments of children.
Higher Dietary Se Intake Is Associated With the Risk of New-Onset Fracture: A National Longitudinal Study for 20 Years
Authors: Zhang Yangchang , Ye Mengliang , Zhao Yong , Xiong Yang , Shen Shisi , Yu Qiuhua , Lu Yanjun , Shi Zumin , Lei Xun
Published: August 2021

Background: The association between dietary selenium (Se) intake and osteoporosis-related fractures remains inconsistent. We aimed to examine the dose relationship between Se intake and incident fracture among Chinese adults.

Methods: The dietary data were retrieved from the China Health and Nutrition Survey conducted between 1991 and 2011, and 17,150 participants aged above 20 were included. A 3-day, 24-h recall of food intake was performed to assess cumulative average dietary Se intake. The fracture was based on self-report in each survey between 1997 and 2011. The association between Se intake and fracture was tested by Cox regression, and the non-linear association was examined by restricted cubic splines (RCS).

Results: There were 976 fracture cases during a mean of 10.2 years follow-up. In a fully adjusted Cox model, across the quartiles of Se intake, the hazard ratios (HRs) for fracture were 1.07 (95% CI .86–1.33), 1 (reference), 1.25 (95% CI 1.02–1.53), and 1.33 (95% CI 1.07–1.65). RCS showed a parabolic association (P non-linear = 0.037) between Se and fracture for men as well as a U-shape dose-response (P non-linear = 0.04) between Se and fracture for subjects living in highly urbanized areas.

Conclusion: In conclusion, there is a non-linear association between selenium intake and fracture, with higher intake associated with increased risk. The shape of the association varies by gender and urbanization level.
Increased Risk of Vitamin D Deficiency Among HIV-Infected Individuals: A Systematic Review and Meta-Analysis
Authors: Wang Yingying , Huang Xiaojie , Wu Yaxin , Li Aixin , Tian Yakun , Ren Meixin , Li Zhen , Zhang Tong , Wu Hao , Wang Wen
Published: August 2021

Background: Human immunodeficiency virus (HIV) infection is a heavy burden worldwide. Observational studies have reported a high prevalence of vitamin D deficiency (VDD) among people living with HIV (PLWH). However, its deficiency is also a global health problem. Therefore, we conducted a meta-analysis and systemic review to compare differences between HIV-infected subjects and non-HIV-infected subjects.

Methods: We searched PubMed, Web of Science, Embase, and Cochrane library. We extracted data, including demographic information, study type, vitamin D-related values, and HIV-related values, ultimately including 15 studies after removing duplicates and screening titles, abstracts, and full texts and finally performing a meta-analysis in terms of vitamin D level and vitamin D deficiency prevalence.

Results: Regarding VDD prevalence, the HIV vs. the non-HIV group had an odds ratio of 1.502 (95% CI, 1.023–2.205; P = 0.038). In the subgroup analysis, the odds ratios were 1.647 (95% CI, 1.020–2.659; P = 0.041; I2 = 94.568) from 7 studies (age over 40), 2.120 (95% CI, 1.122–4.008; P = 0.021; I2 = 0.000) from 2 studies (BMI less than or equal to 25), 1.805 (95% CI, 1.373–2.372; P = 0.042; I2 = 74.576) from 7 studies (latitude <40), 2.120 (95% CI, 1.122–4.088; P = 0.021; I2 = 0.000) from 2 studies (only included male participants), and 2.296 (95% CI, 1.287–4.097; P = 0.005; I2 = 19.927) from 3 studies (only included ART-experienced participants). Thirteen studies were deemed to have moderate quality, while two had high quality.

Conclusions: HIV infected subjects are prone to have VDD compared with general population. ART, older age, lower BMI, lower latitude and male sex may present risk factors for VDD in PLWH.

Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=228096.
Digestion and Absorption of Milk Phospholipids in Newborns and Adults
Authors: Nilsson Åke , Duan Rui-Dong , Ohlsson Lena
Published: August 2021

Milk polar lipids provide choline, ethanolamine, and polyunsaturated fatty acids, which are needed for the growth and plasticity of the tissues in a suckling child. They may also inhibit cholesterol absorption by interacting with cholesterol during micelle formation. They may also have beneficial luminal, mucosal, and metabolic effects in both the neonate and the adult. The milk fat globule membrane contains large proportions of sphingomyelin (SM), phosphatidylcholine (PC), and phosphatidylethanolamine (PE), and some phosphatidylserine (PS), phosphatidylinositol (PI), and glycosphingolipids. Large-scale technical procedures are available for the enrichment of milk fat globule membrane (MFGM) in milk replacement formulations and food additives. Pancreatic phospholipase A2 (PLA2) and mucosal phospholipase B digest glycero-phospholipids in the adult. In the neonate, where these enzymes may be poorly expressed, pancreatic lipase-related protein 2 probably has a more important role. Mucosal alkaline SM-ase and ceramidase catalyze the digestion of SM in both the neonate and the adult. In the mucosa, the sphingosine is converted into sphingosine-1-phosphate, which is both an intermediate in the conversion to palmitic acid and a signaling molecule. This reaction sequence also generates ethanolamine. Here, we summarize the pathways by which digestion and absorption may be linked to the biological effects of milk polar lipids. In addition to the inhibition of cholesterol absorption and the generation of lipid signals in the gut, the utilization of absorbed choline and ethanolamine for mucosal and hepatic phospholipid synthesis and the acylation of absorbed lyso-PC with polyunsaturated fatty acids to chylomicron and mucosal phospholipids are important.
Utilizing Intraoperative Navigated 3D Color Doppler Ultrasound in Glioma Surgery
Authors: Saß Benjamin , Pojskic Mirza , Zivkovic Darko , Carl Barbara , Nimsky Christopher , Bopp Miriam H. A.
Published: August 2021

Background

In glioma surgery, the patient’s outcome is dramatically influenced by the extent of resection and residual tumor volume. To facilitate safe resection, neuronavigational systems are routinely used. However, due to brain shift, accuracy decreases with the course of the surgery. Intraoperative ultrasound has proved to provide excellent live imaging, which may be integrated into the navigational procedure. Here we describe the visualization of vascular landmarks and their shift during tumor resection using intraoperative navigated 3D color Doppler ultrasound (3D iUS color Doppler).

Methods

Six patients suffering from glial tumors located in the temporal lobe were included in this study. Intraoperative computed tomography was used for registration. Datasets of 3D iUS color Doppler were generated before dural opening and after tumor resection, and the vascular tree was segmented manually. In each dataset, one to four landmarks were identified, compared to the preoperative MRI, and the Euclidean distance was calculated.

Results

Pre-resectional mean Euclidean distance of the marked points was 4.1 ± 1.3 mm (mean ± SD), ranging from 2.6 to 6.0 mm. Post-resectional mean Euclidean distance was 4.7. ± 1.0 mm, ranging from 2.9 to 6.0 mm.

Conclusion

3D iUS color Doppler allows estimation of brain shift intraoperatively, thus increasing patient safety. Future implementation of the reconstructed vessel tree into the navigational setup might allow navigational updating with further consecutive increasement of accuracy.
PD-1 and TIGIT Are Highly Co-Expressed on CD8+ T Cells in AML Patient Bone Marrow
Authors: Xu Ling , Liu Lian , Yao Danlin , Zeng Xiangbo , Zhang Yikai , Lai Jing , Zhong Jun , Zha Xianfeng , Zheng Runhui , Lu Yuhong , Li Minming , Jin Zhenyi , Hebbar Subramanyam Sudheendra , Chen Shaohua , Huang Xin , Li Yangqiu
Published: August 2021

Despite the great success of immune-checkpoint inhibitor (ICI) treatment for multiple cancers, evidence for the clinical use of ICIs in acute myeloid leukemia (AML) remains inadequate. Further exploration of the causes of immune evasion in the bone marrow (BM) environment, the primary leukemia site, and peripheral blood (PB) and understanding how T cells are affected by AML induction chemotherapy or the influence of age may help to select patients who may benefit from ICI treatment. In this study, we comprehensively compared the distribution of PD-1 and TIGIT, two of the most well-studied IC proteins, in PB and BM T cells from AML patients at the stages of initial diagnosis, complete remission (CR), and relapse-refractory (R/R) disease after chemotherapy. Our results show that PD-1 was generally expressed higher in PB and BM T cells from de novo (DN) and R/R patients, while it was partially recovered in CR patients. The expression of TIGIT was increased in the BM of CD8+ T cells from DN and R/R patients, but it did not recover with CR. In addition, according to age correlation analysis, we found that elderly AML patients possess an even higher percentage of PD-1 and TIGIT single-positive CD8+ T cells in PB and BM, which indicate greater impairment of T cell function in elderly patients. In addition, we found that both DN and R/R patients accumulate a higher frequency of PD-1+ and TIGIT+ CD8+ T cells in BM than in corresponding PB, indicating that a more immunosuppressive microenvironment in leukemia BM may promote disease progression. Collectively, our study may help guide the combined use of anti-PD-1 and anti-TIGIT antibodies for treating elderly AML patients and pave the way for the exploration of strategies for reviving the immunosuppressive BM microenvironment to improve the survival of AML patients.
Novel Butein Derivatives Repress DDX3 Expression by Inhibiting PI3K/AKT Signaling Pathway in MCF-7 and MDA-MB-231 Cell Lines
Authors: Rampogu Shailima , Kim Seong Min , Shaik Baji , Lee Gihwan , Kim Ju Hyun , Kim Gon Sup , Lee Keun Woo , Kim Myeong Ok
Published: August 2021

Background

Breast cancer is one of the major causes of mortalities noticed in women globally. DDX3 has emerged as a potent target for several cancers, including breast cancer to which currently there are no reported or approved drugs.

Methods

To find effective cancer therapeutics, three compounds were computationally designed tweaking the structure of natural compound butein. These compounds were synthesized and evaluated for their anticancer property in MCF-7 and MDA-MB-231 cell lines targeting DDX3. The in silico molecular docking studies have shown that the compounds have occupied the binding site of the human DDX3 target. Furthermore, to investigate the cell viability effect of 3a, 3b, and 3c on MCF-7 and MDA-MB-231 cell lines, the cell lines were treated with different concentrations of compounds for 24 and 48 h and measured using MTT assay.

Results

The cell viability results showed that the have induced dose dependent suppression of DDX3 expression. Additionally, 3b and 3c have reduced the expression of DDX3 in MCF-7 and MDA-MD-231 cell lines. 3b or 3c treated cell lines increased apoptotic protein expression. Both the compounds have induced the apoptotic cell death by elevated levels of cleaved PARP and cleaved caspase 3 and repression of the anti-apoptosis protein BCL-xL. Additionally, they have demonstrated the G2/M phase cell cycle arrest in both the cell lines. Additionally, 3c decreased PI3K and AKT levels.

Conclusions

Our results shed light on the anticancer ability of the designed compounds. These compounds can be employed as chemical spaces to design new prospective drug candidates. Additionally, our computational method can be adapted to design new chemical scaffolds as plausible inhibitors.
Metformin Alleviates Steatohepatitis in Diet-Induced Obese Mice in a SIRT1-Dependent Way
Authors: Guo Wan-rong , Liu Juan , Cheng Li-dan , Liu Zi-yu , Zheng Xiao-bin , Liang Hua , Xu Fen
Published: August 2021

Metformin is the first-line anti-diabetic drug for type 2 diabetes. It has been found to significantly reduce liver aminotransferase in nonalcoholic fatty liver disease (NAFLD). However, whether metformin improves NAFLD progression remains controversial. Sirtuin 1 (SIRT1), an NAD+-dependent deacetylase, plays a vital role in hepatic steatosis and inflammation. Here, we investigated the effect of metformin on steatohepatitis and the role of SIRT1 in diet-induced obese (DIO) mice. The results showed that metformin significantly reduced body weight and fat mass of DIO mice. In addition, metformin also alleviated adiposity and hepatic steatosis, and greatly upregulated uncoupling protein 1 (UCP1) expression in adipose tissues of DIO mice. Unexpectedly, the effects of metformin on reducing body weight and alleviating hepatic steatosis were not impaired in Sirt1 heterozygous knockout (Sirt1+/−) mice. However, SIRT1-deficiency remarkably impaired the effects of metformin on lowering serum transaminases levels, downregulating the mRNA expression of proinflammatory factors, and increasing the protein level of hepatic Cholesterol 25-Hydroxylase (CH25H), a cholesterol hydroxylase in cholesterol catabolism. In summary, we demonstrated that metformin alleviates steatohepatitis in a SIRT1-dependent manner, and modulation of M1 polarization and cholesterol metabolism may be the underlying mechanism.
Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy
Authors: Dai Feng , Yu Pengfei , Yu Zhenhan , Jiang Hong , Ma Zhijia , Liu Jintao
Published: August 2021

Autophagy has been proved to occur in rats with intervertebral disc degeneration (IVDD). Yiqi Huoxue recipe (YQHXR), an effective therapy of traditional Chinese medicine, was widely used for ruptured lumbar disc herniation under clinical observation. More importantly, YQHXR positively regulated the expression of autophagy-related proteins. However, little is known about the significance of YQHXR in the pathologic process of IVDD. Therefore, this study explored the protective effect of YQHXR based on IVDD rat model through magnetic resonance imaging and histopathologic analysis. Then we evaluated the formation of autophagosomes in the degenerated intervertebral disc by transmission electron microscope. Real-time PCR was used to detect the changes of autophagy-related genes. Western blot and immunoprecipitation were used to assess the protein expression of the autophagy-related pathway. We found that YQHXR-induced autophagy attenuated the release of inflammatory factors. In addition, YQHXR promoted the formation of Beclin1-VPS34 complex to activate autophagy through not only activation of the upstream protein AMPK and upregulation of the deubiquitinase USP13, thus in turn alleviating the development of IVDD. We proposed the potential molecular mechanism of YQHXR on autophagy for the first time, so as to provide a theoretical and experimental basis for the clinical application of YQHXR in the treatment of IVDD-related diseases.
Effect of Two Unique Nanoparticle Formulations on the Efficacy of a Broadly Protective Vaccine Against Pseudomonas Aeruginosa
Authors: Howlader Debaki R. , Das Sayan , Lu Ti , Hu Gang , Varisco David J. , Dietz Zackary K. , Walton Sierra P. , Ratnakaram Siva Sai Kumar , Gardner Francesca M. , Ernst Robert K. , Picking William D. , Picking Wendy L.
Published: August 2021

Pseudomonas aeruginosa is an opportunistic pathogen responsible for a wide range of infections in humans. In addition to its innate antibiotic resistance, P. aeruginosa is very effective in acquiring resistance resulting in the emergence of multi-drug resistance strains and a licensed vaccine is not yet available. We have previously demonstrated the protective efficacy of a novel antigen PaF (Pa Fusion), a fusion of the type III secretion system (T3SS) needle tip protein, PcrV, and the first of two translocator proteins, PopB. PaF was modified to provide a self-adjuvanting activity by fusing the A1 subunit of the heat-labile enterotoxin from Enterotoxigenic E. coli to its N-terminus to give L-PaF. In addition to providing protection against 04 and 06 serotypes of P. aeruginosa, L-PaF elicited opsonophagocytic killing and stimulated IL-17A secretion, which have been predicted to be required for a successful vaccine. While monomeric recombinant subunit vaccines can be protective in mice, this protection often does not transfer to humans where multimeric formulations perform better. Here, we use two unique formulations, an oil-in-water (o/w) emulsion and a chitosan particle, as well as the addition of a unique TLR4 agonist, BECC438 (a detoxified lipid A analogue designated Bacterial Enzymatic Combinatorial Chemistry 438), as an initial step in optimizing L-PaF for use in humans. The o/w emulsion together with BECC438 provided the best protective efficacy, which correlated with high levels of opsonophagocytic killing and IL-17A secretion, thereby reducing the lung burden among all the vaccinated groups tested.
Drug-Induced Liver Disturbance During the Treatment of COVID-19
Authors: Zhai Guanghua , Li Meifen , Wang Ying , Wu Jian
Published: August 2021

An outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurred in Wuhan, China, at the end of 2019. The World Health Organization named the resulting infectious disease as coronavirus disease-2019 (COVID-19). Many studies concluded that patients infected with SARS-CoV-2 have different degrees of liver disturbance. However, the relationship between the drugs used for COVID-19 treatment and liver disturbance remains controversial. It is essential to evaluate the potential liver damage caused by various drugs in order to help guide clinical practice. This review analyzed the effect of drugs on hepatic function during the treatment of COVID-19.
Tunable Nanosensor With a Horizontal Number Eight-Shape Cavity in a MIM Waveguide System
Authors: Xu Daijing , Yan Shubin , Yang Xiaoyu , Wang Jinxi , Wu Xiushan , Hua Ertian
Published: August 2021

Optical devices play an important role in different fields, such as refractive index detection in food processing and the biochemical industry. In our work, a novel nanoscale optical structure, composed of a metal-insulator-metal waveguide with a stub and a horizontal number eight-shape cavity (HNEC), is presented. The transmission properties of this structure are investigated in detail by using finite element method The effects of geometric parameters on sensing performance are studied in detail. Moreover, the influences of an asymmetric resonator caused by shifting central rectangular cavity of HNEC on transmission spectrum are discussed. The changing parameters of HNEC resonator have different effects on different resonance dips. Then, when the parameters of this presented structure are fixed as a of 540 nm, b of 340 nm, S of 0, l of 70 nm and g of 10 nm, this intriguing structure can serve as a refractive index sensor, whose maximum sensitivity can reach 1,500 nm/refractive index unit with a figure of merit of 75. Therefore, this structure will contribute to the development of miniaturization of optical devices.
Numerical Simulation of Thermal Field in Mass Concrete With Pipe Water Cooling
Authors: Zhu Fuxian , Chen Guorong , Zhang Feng , Li Qingwen
Published: August 2021

Water pipe cooling is mainly used to control temperature in the construction of mass concrete structures. It is important to reveal how to accurately stimulate the temperature field of mass concrete under action of this water pipe cooling. This paper presents a new method for this purpose. In this method, the contact surface of the water pipe and the concrete is used as the heat dissipation surface into the control equation and the composite Multiquadrics radial basis function (MQ-RBF) and low-order linear polynomial combination are used to discrete the spatial domain. The heat dissipation surface of the water pipe is included in the boundary conditions so that there is no need to build the refined water pipe modeling. This new method not only reduces the calculation cost but also ensures calculation accuracy. Through four calculation examples, this paper show that the algorithm has advantages in the numerical simulation of the concrete temperature field with water pipe cooling.
Comparative Analysis of Super-Kamiokande Solar Neutrino Measurements and Geological Survey of Israel Radon Decay Measurements
Authors: Sturrock P. A. , Piatibratova O. , Scholkmann F.
Published: August 2021

Analyses of neutrino measurements acquired by the Super-Kamiokande Neutrino Observatory (SK, in operation 1996–2001) and radon decay measurements acquired by the Geological Survey of Israel (GSI, in operation 2007–2017) yield strikingly similar detections of an oscillation with frequency 9.43 ± 0.04 year−1 (SK), 9.44 ± 0.04 year−1 (GSI); amplitude 6.8 ± 1.7% (SK), 7.0 ± 1.0% (GSI); and phase 124 ± 15° (SK), 124 ± 9° (GSI). This remarkably close correspondence supports the proposition that neutrinos may somehow influence nuclear decays. It is interesting to note that an oscillation at this frequency has also been reported by (Alexeyev EN, Gavrilyuk YM, Gangapshev AM, Phys Particles Nuclei, 2018 49(4):557–62) in the decay of 214Po. The physical process responsible for this influence of neutrinos on nuclear processes is currently unknown. Related oscillations in GSI data at 7.45 ± 0.03 year−1 and 8.46 ± 0.03 year−1 suggest that these three oscillations are attributable to a solar core that rotates with a sidereal rotation rate of 8.44 ± 0.03 year−1 about an axis almost orthogonal to that of the convection zone. We briefly discuss possible implications of these results.
The Effect of Fatigue on Single-Leg Postural Sway and Its Transient Characteristics in Healthy Young Adults
Authors: Kozinc Žiga , Trajković Nebojša , Smajla Darjan , Šarabon Nejc
Published: August 2021

Neuromuscular fatigue is known to impair balance ability, which is reflected in increased postural sway during quiet standing tasks. Recently, quantifying transient characteristics of postural sway has been suggested as an approach to obtain additional information regarding postural control. However, this approach is currently vastly unexplored. The purpose of this study was to investigate the effects of fatigue (induced by a repeated change of direction task) on postural sway and its transient characteristics during single-leg standing, including whole-trial estimates and indexes of transient behavior in young healthy active adults. The study involved 28 physically active students (14 females). Single-leg postural sway was recorded for 30s before and after a fatiguing protocol, which consisted of a repeated change of direction tasks. We calculated the traditional whole-trial estimates of postural sway [center-of-pressure (CoP) velocity and amplitude in anterior-posterior (AP) and medial-lateral (ML) directions] and corresponding transient behavior indexes, based on three 10-s intervals. Statistically significant sex×fatigue interaction with medium effect sizes was found for whole-trial CoP velocity in AP (p=0.028;η2=0.17) and ML directions (p=0.019;η2=0.19).Post-hoctest showed that both variables substantially decreased in female participants (p=0.041–0.045;d=0.54–0.56), but remained similar in males (p=0.194–0.294). There were small to medium statistically significant main effects of fatigue on transient index for CoP amplitude in both directions (p=0.042–0.049;η2=0.02–0.14). Notably, CoP AP amplitude increased in the first 10-s interval for males (before fatigue: 5.6±1.3mm; after fatigue: 6.3±1.6mm), while the CoP AP amplitude in the third interval remained similar after fatigue (before fatigue: 5.5±1.4mm; after fatigue: 5.1±1.2mm). In conclusion, the responses to fatigue in terms of postural sway were time interval specific, and there were certain sex-differences in responses to fatigue, which could be related to better ability to adapt balance strategies in females. Moreover, our results demonstrate that the indexes of transient behavior could perhaps detect smaller fatigue-induced changes in postural sway that are seen in whole-trial estimates.
Glutamate 73 Promotes Anti-arrhythmic Effects of Voltage-Dependent Anion Channel Through Regulation of Mitochondrial Ca2+ Uptake
Authors: Shimizu Hirohito , Huber Simon , Langenbacher Adam D. , Crisman Lauren , Huang Jie , Wang Kevin , Wilting Fabiola , Gudermann Thomas , Schredelseker Johann , Chen Jau-Nian
Published: August 2021

Mitochondria critically regulate a range of cellular processes including bioenergetics, cellular metabolism, apoptosis, and cellular Ca2+ signaling. The voltage-dependent anion channel (VDAC) functions as a passageway for the exchange of ions, including Ca2+, across the outer mitochondrial membrane. In cardiomyocytes, genetic or pharmacological activation of isoform 2 of VDAC (VDAC2) effectively potentiates mitochondrial Ca2+ uptake and suppresses Ca2+ overload-induced arrhythmogenic events. However, molecular mechanisms by which VDAC2 controls mitochondrial Ca2+ transport and thereby influences cardiac rhythmicity remain elusive. Vertebrates express three highly homologous VDAC isoforms. Here, we used the zebrafish tremblor/ncx1h mutant to dissect the isoform-specific roles of VDAC proteins in Ca2+ handling. We found that overexpression of VDAC1 or VDAC2, but not VDAC3, suppresses the fibrillation-like phenotype in zebrafish tremblor/ncx1h mutants. A chimeric approach showed that moieties in the N-terminal half of VDAC are responsible for their divergent functions in cardiac biology. Phylogenetic analysis further revealed that a glutamate at position 73, which was previously described to be an important regulator of VDAC function, is sevolutionarily conserved in VDAC1 and VDAC2, whereas a glutamine occupies position 73 (Q73) of VDAC3. To investigate whether E73/Q73 determines VDAC isoform-specific anti-arrhythmic effect, we mutated E73 to Q in VDAC2 (VDAC2E73Q) and Q73 to E in VDAC3 (VDAC3Q73E). Interestingly, VDAC2E73Q failed to restore rhythmic cardiac contractions in ncx1 deficient hearts, while the Q73E conversion induced a gain of function in VDAC3. In HL-1 cardiomyocytes, VDAC2 knockdown diminished the transfer of Ca2+ from the SR into mitochondria and overexpression of VDAC2 or VDAC3Q73E restored SR-mitochondrial Ca2+ transfer in VDAC2 deficient HL-1 cells, whereas this rescue effect was absent for VDAC3 and drastically compromised for VDAC2E73Q. Collectively, our findings demonstrate a critical role for the evolutionary conserved E73 in determining the anti-arrhythmic effect of VDAC isoforms through modulating Ca2+ cross-talk between the SR and mitochondria in cardiomyocytes.
Genomic-Assisted Marker Development Suitable for CsCvy-1 Selection in Cucumber Breeding
Authors: Kahveci Erdem , Devran Zübeyir , Özkaynak Ercan , Hong Yiguo , Studholme David J. , Tör Mahmut
Published: August 2021

Cucumber is a widely grown vegetable crop plant and a host to many different plant pathogens. Cucumber vein yellowing virus (CVYV) causes economic losses on cucumber crops in Mediterranean countries and in some part of India such as West Bengal and in African countries such as Sudan. CVYV is an RNA potyvirus transmitted mechanically and by whitefly (Bemisia tabaci) in a semipersistent manner. Control of this virus is heavily dependent on the management of the insect vector and breeding virus-resistant lines. DNA markers have been used widely in conventional plant breeding programs via marker-assisted selection (MAS). However, very few resistance sources against CVYV in cucumber exist, and also the lack of tightly linked molecular markers to these sources restricts the rapid generation of resistant lines. In this work, we used genomics coupled with the bulked segregant analysis method and generated the MAS-friendly Kompetitive allele specific PCR (KASP) markers suitable for CsCvy-1 selection in cucumber breeding using a segregating F2 mapping population and commercial plant lines. Variant analysis was performed to generate single-nucleotide polymorphism (SNP)-based markers for mapping the population and genotyping the commercial lines. We fine-mapped the region by generating new markers down to 101 kb with eight genes. We provided SNP data for this interval, which could be useful for breeding programs and cloning the candidate genes.
The Complexity of Modulating Anthocyanin Biosynthesis Pathway by Deficit Irrigation in Table Grapes
Authors: Afifi Maha , Obenland David , El-kereamy Ashraf
Published: August 2021

Deficit irrigation (DI) is an irrigation scheduling technique that is used in grapes to improve red color development; however, results are not always satisfactory in table grapes. The red color in grapes is mainly due to the plant pigment anthocyanin. In the present study, the anthocyanin biosynthesis in Scarlet Royal grapes (Vitis viniferaL.) grown in the San Joaquin and Coachella Valleys, and subjected to two different DI strategies was investigated. The objective of this study was to identify potential regulatory factors that may lead to potential treatments to improve red color in table grapes, especially under warm climate conditions. In both locations, DI induced the expression of several genes involved in three major pathways that control the red color in table grapes: anthocyanin biosynthesis, hormone biosynthesis, and antioxidant system. DI at veraison induced anthocyanin accumulation and enhanced red color in berries at harvest time. However, anthocyanin accumulation was lower at the Coachella Valley compared to the San Joaquin Valley. The lower level of anthocyanin was associated with lower expression of critical genes involved in anthocyanin biosynthesis, such asflavonoid-3-O-glucosyltransferase(UFGT), myb-related regulatory gene (R2R3-MYB)(MYBA1),basic helix-loop-helix(bHLH) (MYCA1) and thetryptophan-aspartic acid repeat (WDR or WD40) proteins(WDR1). Further, gene expression analysis revealed the association of ABA biosynthesis gene9-cis-epoxycarotenoid dioxygenase(NCED1),1-aminocyclopropane-1-carboxylic acid oxidase(ACO3), and the gibberellic acid (GA) catabolic geneGA2 oxidase(GA2ox1) in the induction of anthocyanin biosynthesis. An increase in thechalcone synthasegene (CHS2) was observed in response to DI treatments in both sites. However,CHS2expression was higher in Coachella Valley after ending the DI treatment, suggesting the involvement of environmental stress in elevating its transcripts. This data was also supported by the lower level of antioxidant gene expression and enzyme activities in the Coachella Valley compared to the San Joaquin Valley. The present data suggested that the lack of grape red coloration could partially be due to the lower level of antioxidant activities resulting in accelerated anthocyanin degradation and impaired anthocyanin biosynthesis. It seems that under challenging warmer conditions, several factors are required to optimize anthocyanin accumulation via DI, including an active antioxidant system, proper light perception, and hormonal balance.
Neural Responses During Emotion Transitions and Emotion Regulation
Authors: Hao Yu , Yao Lin , Evans Gary W.
Published: August 2021

Why are some people more susceptible to interference from previous emotional stimuli? Neural mechanisms underlying emotion regulation are typically studied with one-off positive or negative stimuli. Less is known about how they operate during dynamic emotional experiences, which more closely resemble how emotions occur in real life. Therefore, we investigated the interaction among temporal context, stimulus content, and regulatory strategy. Image sequences included either neutral to negative emotion or negative to neutral emotion. Participants were instructed to either passively watch the emotional stimuli or apply cognitive reappraisal during the image sequences presentation. Participants also reported their habitual use of cognitive reappraisal in their daily lives on a standard scale. We measured functional connectivity (FC) with electroencephalography (EEG) source localization. A three-way interaction suggested that, in addition to momentary emotional content and regulatory effort, the temporal context of stimuli impacts the FC between the ventromedial prefrontal cortex (vmPFC) and the ventral anterior cingulate cortex (ACC) in both alpha and beta frequency bands. In the reappraisal condition—but not the passive watch conditions—, individual differences in habitual reappraisal were manifested in the FC of vmPFC-ACC in alpha band. Emotion transitions may be more demanding because prefrontal-posterior FC in the beta band decreased during emotion transitions regardless of emotional content or regulation efforts. Flexible emotion regulation enables the recruiting of neural activities in response to the content of dynamic, ever-changing experiences encountered in daily life. Studying brain responses to dynamic emotional stimuli may shed light on individual differences in adaptation and psychological health. It also provides a more ecologically valid assessment of emotion regulation.
Bedtime Smart Phone Usage and Its Effects on Work-Related Behaviour at Workplace
Authors: Ellahi Abida , Javed Yasir , Begum Samina , Mushtaq Rabia , Rehman Mobashar , Rehman Hafiz Mudassir
Published: August 2021

The over usage and over dependency on digital devices, like smartphones, has been considered as a growing international epidemic. The increased dependency on gadgets, especially smartphones for personal and official uses, has also brought many detrimental effects on individual users. Hence it is vital to understand the negative effects of smartphone usage on human. Therefore, this study aims to investigate the effects of bedtime smartphone usage on work performances, interpersonal conflicts, and work engagement, via the mediating role of sleep quality among employees. Using a cross-sectional study design, a questionnaire-based field survey was conducted on 315 employees who participated as respondents. The results confirmed the negative effects of bedtime smartphone usage on sleep quality. Along with it, the effects of sleep quality on work performances, work engagements and interpersonal conflicts were also proven to be statistically significant. Regarding the mediating role of sleep quality, it was empirically evident that sleep quality mediates the relationship between bedtime smartphone usage with work performances and interpersonal conflicts. The findings revealed that bedtime smartphone usage reduces sleep quality among the employees, resulting in lower work performances and engagements while contributing to higher interpersonal conflicts. The findings concluded that smartphone usage before sleep increases the prospects of employees to be less productive, less engaged, and have more workplace conflicts. The findings warrant the continued managerial as well as academic research attention, as the smartphones are now used by many organisations to run businesses as well.
No Moral Wiggle Room in an Experimental Corruption Game
Authors: Balafoutas Loukas , Sandakov Fedor , Zhuravleva Tatyana
Published: August 2021

Recent experimental evidence reveals that information is often avoided by decision makers in order to create and exploit a so-called “moral wiggle room,” which reduces the psychological and moral costs associated with selfish behavior. Despite the relevance of this phenomenon for corrupt practices from both a legal and a moral point of view, it has hitherto never been examined in a corruption context. We test for information avoidance in a framed public procurement experiment, in which a public official receives bribes from two competing firms and often faces a tradeoff between maximizing bribes and citizen welfare. In a treatment where officials have the option to remain ignorant about the implications of their actions for citizens, we find practically no evidence of information avoidance. We discuss possible reasons for the absence of willful ignorance in our experiment.
Into the Unknown: Uncertainty and Procrastination in Students From a Life History Perspective
Authors: Lim Amy J. , Javadpour Sasha
Published: August 2021

While existing findings have established an increase in procrastination among students in higher education during COVID-19, they do not elucidate how COVID-19 has effected an increase in procrastination. Drawing upon previous findings and employing a life history framework, this paper proposed that the increase in procrastination may be attributed to the heightened levels of uncertainty in the pandemic. Additionally, this paper examined life history strategy as the psychological mechanism underlying the relation between uncertainty and procrastination. By collecting data across two school semesters in a university (N = 253), we found that uncertainty and procrastination did not differ between the semester where changes were abruptly imposed to stem the spread of COVID-19 and the following semester. Our findings also revealed that uncertainty predicted procrastination, and that life history strategy mediated the relation between uncertainty and procrastination. Specifically, uncertainty was associated with a faster life history strategy, which was positively associated with procrastination. By shedding light on the psychology behind the effect of uncertainty on procrastination, the findings of this paper hold important implications for the design of procrastination interventions for the uncertain climate during the pandemic and “the new normal” post COVID-19.
Synchronous Mindfulness in Motion Online: Strong Results, Strong Attendance at a Critical Time for Health Care Professionals (HCPs) in the COVID Era
Authors: Klatt Maryanna , Bawa Rani , Gabram Olivia , Westrick Alexis , Blake Amanda
Published: August 2021

Mindfulness in Motion (MIM) is an organizationally-sponsored mindfulness program for employees at a large academic health center that consistently produces significant reductions in burnout and perceived stress, alongside significant increases in work engagement and resilience. This study compared outcome measures of a synchronous virtual delivery of MIM, necessitated by COVID-19, to traditional in-person delivery of MIM. Outcome measures from the virtual COVID (AU20, WI21, SP21) MIM cohorts (n = 99) were compared with the in-person Pre-COVID (SP19, AU19, WI20) MIM cohorts (n = 124). Both Pre-COVID and COVID cohorts had similar attendance rates with an average attendance of 84 and 80%, respectively. Qualitative analysis of COVID cohorts reported community support during COVID as a substantial intervention benefit, which was important at a time when isolation dominated the healthcare professional experience. Total burnout was determined by scores on the subscales of the Maslach Burnout Inventory (MBI). There were no significant differences in depersonalization (p = 0.3876) and personal accomplishment (p = 0.1519) changes between Pre-COVID and COVID cohorts, however there was a significant difference in emotional exhaustion (p = 0.0315), with COVID cohorts improving more. In both Pre, and COVID cohorts, the percentage of people meeting burnout criteria from pre to post between groups were similar, yielding a non-significant difference (p = 0.2950). The Connor Davidson Resiliency Scale (CDRS) and Utrecht Work Engagement Scale (UWES) also produced no significant differences between groups (p = 0.4259, p = 0.1984, respectively). The Perceived Stress Scale (PSS) though yielded significant differences in reduction between groups (p = 0.0405), again with COVID cohorts showing greater improvement. Results of the first synchronous, virtually delivered MIM cohorts reflect that participants achieved very similar results and that MIM created a community in a time when it was greatly needed due to pandemic healthcare professional stress.
Conspiratorial Thinking During COVID-19: The Roles of Paranoia, Delusion-Proneness, and Intolerance of Uncertainty
Authors: Larsen Emmett M. , Donaldson Kayla R. , Liew Megan , Mohanty Aprajita
Published: August 2021

The COVID-19 global pandemic has left many feeling a sense of profound uncertainty about their world, safety, and livelihood. Sources espousing misinformation and conspiracy theories frequently offer information that can help make sense of this uncertainty. Individuals high in intolerance of uncertainty (IU) may be particularly impacted by the impoverished epistemic environment and may thus be more drawn to conspiratorial thinking (CT). In the present work, we show across 2 studies (N = 519) that COVID-19-specific CT is associated with higher levels of IU as well as delusion-proneness, and paranoia. Furthermore, delusion-proneness and paranoia explained the relationship between IU and CT and emerged as independent partial correlates of CT even when controlling for other facets of schizotypy. In contrast, anxiety did not explain the relationship between IU and CT. Overall, our findings highlight the importance of individual differences in IU, delusion-proneness and paranoia in the development of CT in the context of the acute uncertainty of a global crisis, in which conspiracy theories are more prevalent and salient. Informational intervention designs may benefit from leveraging the body of work demonstrating the efficacy of targeting IU to incite meaningful changes in thinking.
Validation of the Rage Attack Questionnaire-Revised (RAQ-R) in a Mixed Psychiatric Population
Authors: Palm Lisa , Haas Martina , Pisarenko Anna , Jakubovski Ewgeni , Müller-Vahl Kirsten R.
Published: August 2021

Rage Attacks (RA) represent a clinically relevant symptom in patients with different psychiatric disorders. However, only recently the Rage Attack Questionnaire Revised (RAQ-R, 22 items, range, 0–66) has been developed as a new instrument for the assessment of RA. This study aimed to validate the RAQ-R in a large mixed psychiatric and psychosomatic sample. We tested internal consistency, convergent and discriminant validity as well as factor structure. In order to further explore the relationship of RA to other psychiatric symptoms, we calculated Pearson correlations between the RAQ-R and several other self-assessments including measurements for general psychological distress, quality of life, depression, anxiety, attention deficit/hyperactivity disorder (ADHD), impulsivity, and self-regulation abilities. Most relevant predictors of RA were examined in a multiple regression with stepwise elimination. In order to assess the manifestation of RA in different psychiatric disorders, group differences between diagnostic categories and healthy controls were calculated. Additionally, psychiatric patients were compared to patients with Tourette syndrome along RAQ-R scores. Data from healthy subjects and patients with Tourette syndrome were obtained from a previous study of our group. In this study, we included 156 patients with a wide and typical spectrum of psychiatric diseases. The RAQ-R was found to have excellent internal consistency and strong construct validity in this sample (Cronbach's α = 0.97, Average Variance Extracted = 0.58). Thus, the RAQ-R was shown to be a psychometrically sound assessment of RA in patients with different psychiatric disorders. Close constructs to RA were found to be aggression and hostility (r = 0.68) as well as low frustration tolerance and impulse control (r = 0.69). Compared to healthy controls, RA were significantly more common in the psychiatric sample (p < 0.001). More specifically, RAQ-R scores in all diagnostic categories assessed were higher compared to controls. Highest scores and effect sizes were found in patients with ADHD and borderline personality disorder (p < 0.001). Our results suggest that RA are a common and relevant symptom in many psychiatric disorders. As depression and RA showed only a moderate relation, RA should be distinguished from the concept of anger attacks, which are described as a core symptom of depression.
Lifestyle and Risk Factors in Hemorrhoidal Disease
Authors: De Marco Stefania , Tiso Domenico
Published: August 2021

Constipation, a low fiber diet, a high Body Mass Index, pregnancy, and a sedentary lifestyle are often assumed to increase the risk of hemorrhoidal disease (HD). However, evidence regarding these factors is controversial. This mini-review aims to examine and critically analyze the association between main risk factors and the prevalence of HD, focusing both on the patient's clinical history and on a tailored treatment. Moreover, some practical suggestions about lifestyle and conservative approaches are given to help clinicians in the management of patients with HD and to obtain the best results from therapy.