bluuebunny/crossref_metadata_2025 huggingface dataset:
Sosyal Bilgiler Öğretiminde Proje Tabanlı Öğrenme Yaklaşımının Girişimcilik Becerilerine Etkisi: Bir Karma Yöntem Araştırması
Authors: ÜLKER Battal Gazi , BEKTAŞ Özlem
Published: July 2023

Bu araştırmada, Sosyal Bilgiler öğretiminde Proje Tabanlı Öğrenme (PTÖ) Yaklaşımının ortaokul öğrencilerinin girişimcilik becerilerine etkisinin incelenmesi amaçlanmıştır. Bu amaç doğrultusunda hem nitel hem de nicel veri toplama tekniklerinin birlikte kullanıldığı karma yöntem desenlerinden sıralı açıklayıcı karma desen kullanılmıştır. Nicel veri toplama aracı olarak, Eroğlu, Deveci ve Gürdoğan Bayır (2020) tarafından hazırlanan “Ortaokul Öğrencilerine Yönelik Girişimcilik Ölçeği” ve araştırmacı tarafından hazırlanan Kişisel Bilgi Formu kullanılmıştır. Nitel veriler ise yarı yapılandırılmış görüşme formu kullanılarak elde edilmiş ve içerik analizi ile yorumlanmıştır. Araştırmada çalışma grubu seçkisiz olmayan örnekleme yöntemlerinden uygun örnekleme yöntemi kullanılarak belirlenmiştir. Bu kapsamada Erzincan il merkezinde bir ortaokulda 5. sınıfta öğrenim görmekte olan iki sınıftan biri deney grubu (N14), diğeri ise kontrol grubu (N14) olarak seçilmiştir. Uygulama sürecinde dersler deney grubunda Proje Tabanlı Öğrenme Yaklaşımı çerçevesinde hazırlanan “Erzincan KOSGEB ile Bilgileniyorum”, “Erzincan OSB’yi Keşfediyorum”, “Üretiyorum-Satıyorum” ve “Kadın Girişimcilerle Buluşuyorum” başlıkları altında 4 ayrı proje kapsamında işlenirken, kontrol grubunda ise müfredata uygun bir şekilde işlenmiştir. Araştırma sonucunda, deney ve kontrol grubu öntest ve sontest puanları karşılaştırıldığında, deney grubu öğrencilerinin girişimcilik becerilerinin kontrol grubu öğrencilerine göre istatiksel olarak anlamlı bir şekilde farklılaştığı sonucuna ulaşılmıştır. Araştırmanın nitel boyutuna yönelik sonuçlara göre; Proje Tabanlı Öğrenme Yaklaşımının yaratıcılık, yenilikçilik, risk alma-cesaretli olma ve başarılı girişimciler ile meslekleri tanımada etkili bir yöntem olduğu gözlemlenmiştir. Sonuç olarak, Sosyal Bilgiler öğretiminde Proje Tabanlı Öğrenme Yaklaşımının, ortaokul öğrencilerine girişimcilik becerilerini kazandırmada etkili bir yaklaşım olduğu söylenebilir.
Babesia equi  and Trypanosoma vivax  infections in donkeys
Authors: Kyewalabye Kaggwa E. , Lawal I.A.
Published: July 2023

Six ânes (Equus asinus) ont été achetés sur des marchés locaux. Avant et pendant les infections par Trypanosoma vivax, pour détecter B. equi, les techniques suivantes ont été employées : étalement de sang et gouttes épaisses, prise de température, centrifugation hématocrite, volume globulaire total, numération globulaire et immunofluorescence indirecte. Pour cette dernière épreuve, un conjugué anti-cheval a été utilisé. En dépit des parasitémies à T. equi ou à T. vivax, la température des ânes est restée inférieure à 38,5¼C. Le volume globulaire total s'est abaissé davantage lors de l'infection à B. equi que lors de celle à T. vivax. Quatre ânes sur 6 avaient des anticorps contre B. equi alors que 2 seulement présentaient une parasitémie décelable. Le traitement, soit avec le BérénilND, soit avec l'ImizolND, a éliminé la parasitémie décelable à B. equi, et le test d'immunofluorescence indirecte était négatif 35 à 45 jours après le traitement. Cependant des rechutes se sont produites 60 à 70 jours après traitement. Dans deux cas, les titres sérologiques étaient inférieurs à 1 : 40 (c'est-à-dire négatifs) alors que la parasitémie était manifeste.
O Recife mal-assombrado
Authors: Silva Dalexon Sérgio da
Published: July 2023

Este trabalho promove um gesto de leitura e interpretação, por meio da análise discursiva de duas imagens de terror, extraídas do site Catamaran, que (se)dizem sobre o Recife mal-assombrado. Nelas vemos a encenação feita de lendas urbanas, num passeio turístico de Catamaran, sobre as águas do Rio Capibaribe, que cortam as ruas do centro da cidade e, entre memória, paráfrase e polissemia, apresentam a Veneza Brasileira como a cidade mais assombrada do Brasil. Assim, tomando essas imagens como materialidades discursivas, por meio da Análise Materialista do Discurso de vertente pecheuxtiana, este artigo analisa os deslocamentos de sentidos e seus efeitos produzidos na circulação delas, bem como, o modo como (se)dizem saberes discursivos a partir dessas imagens, que trazem já-ditos e funcionamentos constitutivos na exterioridade pela historicidade, inscritos numa rede de memórias, na relação sempre constitutiva entre paráfrase e polissemia. Nesse entremeio, este artigo traz como principal contribuição mostrar o funcionamento discursivo da capital pernambucana, dita de outro modo, no Dark Tourism.
Hiper(in)visibilidade e populismo urbano-midiático em São Paulo
Authors: Demuru Paolo
Published: July 2023

O artigo aborda as estratégias semiopolíticas por meio das quais se articula, hoje, em São Paulo, a relação entre poder e visibilidade. Com base no arcabouço teórico-metodológico da semiótica greimasiana e pós-greimasiana, defendem-se as seguintes teses: (i) São Paulo é caracterizada por uma verdadeira hipertrofia da visão, que se manifesta, ao mesmo tempo, sob o perfil arquitetônico-urbanístico e midiático; (ii) em São Paulo, o poder é hipervisível e transparente (iii); tal florilégio de imagens, olhares e perspectivas produz vastas áreas de invisibilidade topológica, midiática e social; (iv) o regime de visibilidade que impera na capital paulista é um regime de visibilidade populista.
Espiralando o cima-baixo do mundo
Authors: Santos Filho José Teixeira dos , Rosa Laís Cardoso da
Published: July 2023

Em outubro de 2022, como parte das atividades do Projeto de Extensão Poéticas do corpo em paisagens pandêmicas: criar comunidades para reavivar afetos com a cidade, oferecemos a oficina “como caminhar mais próximo do chão” para alunos e ex-alunos da Unicamp, em que buscamos construir uma relação mais próxima com o chão sob nossos pés e com os chãos de nossas histórias. Neste artigo, compartilhamos não só a experiência dividida com os participantes na experimentação do movimento espiral entre ser corpo horizontal e vertical, mas o próprio processo criativo da oficina, nascido em nosso encontro e no desejo comum de contracolonizar os nossos pensamentos-ações enquanto pessoas educadoras-artistas-pesquisadoras.
The Design and Simulation of a Reversible Quantum-Dot Cellular Automata-based Arithmetic Logic Unit
Authors: Alharbi Mohammed , Edwards Gerard , Stocker Richard
Published: July 2023

Quantum-dot cellular automata (QCA) are a promising nanoscale computing technology that exploits the quantum mechanical tunneling of electrons between quantum dots in a cell and electrostatic interaction between dots, in neighboring cells. QCA can achieve higher speed, lower power, and smaller areas than conventional complementary metal–oxide–semiconductor (CMOS) technology. Developing QCA circuits in a logically and physically reversible manner can provide exceptional reductions in energy dissipation. The main challenge of reversible QCA design is to maintain reversibility down to the physical level. The arithmetic logic unit (ALU) is an essential component of the central processing unit (CPU) of a computer. It performs various arithmetic and logical operations on the data that the CPU processes. Current QCA ALU designs are either irreversible or logically reversible; however, they lack physical reversibility, a crucial requirement to increase energy efficiency. In this paper, we present a novel multilayer design for a logically and physically reversible QCA ALU based on majority gates as the key components, which can perform 16 different operations. We use QCADesigner-E software to simulate and evaluate energy dissipation. The proposed logically and physically reversible QCA ALU offers an improvement of 88.8% in energy efficiency. Compared to the next most efficient 16-operation QCA ALU, this ALU uses 51% fewer QCA cells and 47% less area.
Gravity as a Result of Symmetry between Matter Energy and Space Energy
Authors: Sorli Amrit , Čelan Štefan
Published: July 2023

In physics, symmetry is mostly about the symmetry between elementary particles, between matter and antimatter. In the universe, we have two fundamental types of energy: energy of matter and energy of space. These two energies have a symmetry that is not geometry type, not electric charge type, it is energy type. Every physical object with mass m symmetrically diminishes the energy density of space in its center exactly for the amount of its mass. This symmetry of matter and space energies is the physical source of gravity that is also valid in the centre of black holes.
Are Smart Cities Successfully Accepted by all Citizens? A New Recommender System Applied to Tourist Mobile Technologies
Authors: Marín Díaz Gabriel , Galdón Salvador José Luis , Galán Hernández José Javier
Published: July 2023

Due to the irruption of new technologies in cities such as mobile applications, geographic infor-mation systems, Big Data, Internet of Things (IoT) or Artificial Intelligence (AI), new approaches to citizen management are being developed with the aim of adapting citizen services to this new en-vironment. These new services can enable city governments and businesses to offer their citizens a truly immersive experience that facilitates their day-to-day lives and ultimately improves their quality of life. In this sense, it is important to emphasize that all investments in infrastructure and technological developments in smart cities will be wasted if the citizens for whom they have been created eventually do not use them for whatever reason. To avoid these kinds of problems, the citizen's level of adaptation to the technologies should be evaluated. However, although much has been studied about new technological developments, studies to validate the actual impact and user acceptance of these technological models are much more limited. This paper tries to fill this gap presenting a new model of recommender system based in the most cited and used model in the scientific and academic literature: the Technology Acceptance Model (TAM) and using the most cited and agreed upon criteria in the existing literature. To accomplish the objective, this study in-troduces a novel recommender system that utilizes a fuzzy 2-tuple linguistic model in conjunction with the analytic hierarchy process (AHP) method to prioritize and personalize the relationship between tourists and smart cities. The methodology proposed in this paper was tested and validated in a case of study through different clusters to derive global recommendations tailored to each specific cluster. The main findings reveal that the use of technology is closely linked to the ability to enjoy personalized experiences in the realm of Smart Cities and Smart Tourism. As future works, authors recommend extending the recommender system model to any cluster of tourists using the proposed methodology and evaluate also other kind of disruptive technologies such as artificial intelligence (AI) in supporting the citizens.
Prediction of Seizure Stages Based on Electroencephalography Signals and Machine Learning
Authors: Dutta Kusumika Krori , Manohar Premila , K Indira
Published: July 2023

Electroencephalography (EEG) is essential for tracking brain activity and identifying seizure effects. However, epileptic behaviour can only be detected after a specialist has carefully analysed all EEG recordings along with a proper history of the patient. A skilled physician is required for the right epilepsy diagnosis and therapy. But most of the time, patients visit the clinician in the interictal stage with no proper history documented. Therefore, it was essential to the automatic prediction of stages of seizure. K nearest neighbours (KNN) and random forest (RF) models using raw EEG signals, preictal, ictal, postictal, and interictal stages were identified in this study. The possibility of these characteristics is explored by examining how well time-domain signals work in the prediction of epileptic stages using intracranial EEG datasets from Freiburg Hospital (FH), Children's Hospital Boston-Massachusetts Institute of Technology (CHB-MIT), and Temple University Hospital (TUHEEG). To test the viability of this approach, two different types of simulations were carried out on three binary classifications (interictal vs. preictal, interictal vs. ictal, preictal vs. postictal, and interictal vs. postictal), and one four-class problem (interictal vs. preictal vs. ictal vs. postictal) was performed for each model. The average accuracy when using time-domain signals in the FH database was 90.5% and 75.0%; CHB-MIT was 92.87% and 75.9%; and TUHEEG was 94.46% and 76.8%, respectively, for the KNN and RF models.
Entrevista com Katherine Blouin. A História Antiga para além do Eurocentrismo: indigenizando o ensino e a pesquisa
Authors: Silva Uiran Gebara da , Frizzo Fabio , Gontijo Leite Priscilla
Published: July 2023

Esta entrevista compõe o dossiê Antiguidade na Cultura Histórica: experiênciasescolares e públicas da Revista História Hoje. Este dossiê foi motivado,por um lado, pelos desdobramentos recentes na educação básica brasileira –instituição de uma base comum curricular nacional e a reforma do EnsinoMédio, numa disposição transversal das disciplinas de Humanidades (Filosofia,Sociologia, História) – e, por outro, pelos avanços nas pesquisas sobre ensinode História Antiga.O ensino superior brasileiro recebeu grande influência do modelo francêse, neste contexto, o campo da História em particular foi dependente dahistoriografia francesa, antes da recente imposição da linguagem e do padrãode pesquisa anglo-saxões.Para atuar como professor de História para alunos de 11 a 17 anos, deve--se ter a graduação em História, que dura em média 4 anos. O curso é compostopor disciplinas geralmente divididas em três grupos: primeiro, disciplinasque apresentam os eventos e processos sociais de sociedades passadasorganizadas em torno da linha do tempo cronológica quadripartite (HistóriaAntiga, História Medieval, História Moderna e História Contemporânea); segundo,disciplinas sobre Teoria e Metodologia da História; terceiro, disciplinas sobre ensino de História, que tomam uma maior parte no currículo daquelesque optam por licenciatura em História. Nesse modelo, as graduaçõescostumam ter de uma a duas disciplinas dedicadas à História Antiga, usualmentefocando em “Antiguidade Clássica” ou “Civilização Greco-Romana”.A especialização para atuar na História Antiga acontece na pós-graduaçãocom mestrados e doutorados em temas de História Antiga.Na Educação Superior Brasileira, Estudos Clássicos é um termo que frequentementefica restrito aos cursos de graduação e pós-graduação em Letrasespecializados em grego ou latim. Os estudantes de História que desejam melhorarsua formação geralmente a complementam cursando disciplinas de LetrasClássicas, Filosofia Antiga e (mais raramente) Arqueologia Clássica. Apesardo diálogo entre classistas, historiadores e filósofos, a departamentalizaçãono campo de trabalho restringe a atuação dos profissionais a sua graduação.
O uso do lúdico no contexto da pandemia: o jogo educativo digital para o ensino de História dos 4º e 7º anos do Ensino Fundamental
Authors: Sobrinho Junior João Ferreira , Moraes Cristina de Cássia Pereira
Published: July 2023

O artigo se propõe a identificar aplicaçõespráticas à utilização do jogo HistóriasConectadas – que trata da expansãomarítima portuguesa no século XV –para o ensino de História dos 4º e 7ºanos do Ensino Fundamental. Com finsa se alcançar esse objetivo, o percursometodológico se fundamentou em umapesquisa exploratória com a utilizaçãode levantamento bibliográfico e documental,com abordagem qualitativa. E,como resultado da pesquisa, identificamosque do jogo Histórias Conectadasforam levantados alguns elementos quepodem ser utilizados para ensino deHistória, fundamentado nos objetos diretrizadoresda Base Nacional ComumCurricular – BNCC. Conclui-se entãoque o jogo em questão buscou representaro contexto da época (ambiental, histórico,político, estruturas básicas, fauna e flora, dentre outros) e assim, este podeoferecer variados caminhos a serem exploradospelo professor, de acordo comseu planejamento e/ou plano de aula.
A escrita da Pré-História em compêndios escolares de História no Brasil: o caso João Ribeiro (1892)
Authors: Guerra Lolita Guimarães , Barnabé Luis Ernesto
Published: July 2023

No decorrer do século XIX, surge e sedesenvolve o conhecimento da Pré-Históriacomo período mais remoto daHistória e área específica de conhecimento.A circulação atlântica de notíciassobre ideias, conceitos, sítios e vestígiospré-históricos, junto à construçãode uma filiação ocidental para o nossopaís, enquanto nação moderna, resultamna primeira prescrição oficial daPré-História sob a República. O compêndioHistoria Antiga, de João Ribeiro,adotado pelo Ginasio Nacional (nomerepublicano dado ao Imperial Collegiode Pedro II) em 1892, é um documentocentral para a compreensão do processoda escrita da Pré-História escolar nocontexto daquele colégio, na década de1890. Nele, observamos a construção deuma Pré-História nos moldes do debatecientífico da época. Este trabalho situasua prescrição nos Programas de ensino docolégio e identifica as linhas mestrasde sua Pré-História.
Use of Microscopic Tubular Decompression in Thoracic Ossification of the Ligamentum Flavum: A Retrospective Analysis of Outcomes
Authors: Kulkarni Arvind G. , Patil Shankargouda R. , Sharma Sagar
Published: July 2023

Objective: This study retrospectively analyzed the outcomes of microscopic tubular decompression (MTD) in thoracic spinal stenosis due to ossification of the ligamentum flavum.Methods: Twelve patients who had symptomatic thoracic spinal stenosis with or without co-existing spinal stenosis at other spinal regions were included in the study. The inclusion criteria were complaints of numbness or paresthesia below the affected level, gait difficulties, back pain, hyperreflexia and increased muscle tone upon clinical examination with magnetic resonance imaging evidence of ligamentum flavum thickening resulting in thoracic canal stenosis. The whole spine was evaluated for tandem stenosis. Patients with other causes of thoracic spinal stenosis due to a tumor or malignancy and multiple-level thoracic spinal stenosis were excluded. Results: The patients’ mean age was 59 years. There were 9 men and 3 women. The average duration of symptoms was 8.6 months, and the average follow-up period was 15.4 months. The mean preoperative Nurick score was 3.83 and mean postoperative Nurick score was 1.5. Postoperatively, 1 patient presented with bilateral lower limb weakness due to local hematoma, which was promptly evacuated with open decompression.Conclusion: MTD allows a magnified surgical field while minimizing disruption to the surrounding soft tissue and bone structures and enables complete decompression of the spinal cord with minimal alteration to the biomechanical strength of the vertebral column. The advantages of tubular decompression include decreased blood loss, reduced hospital stay, early mobilization, and less muscle injury. Rapid recovery from surgical treatment is another potential advantage of this approach.
Fully-Endoscopic Posterior Cervical 2-Level Foraminotomy With Total Pediculectomy for Adjacent-Level Foraminal Stenosis in Severe Degenerative Spondylolisthesis: A Technical Note With 1-Year Follow-up
Authors: Kim Hyeun-Sung , Raorane Harshavardhan Dilip , An Jin Woo , Yi Yeon Jin , Jang Il Tae
Published: July 2023

Although the conventional anterior approach is the gold-standard procedure for multilevel cervical spondylotic radiculopathy, the fully-endoscopic posterior approach has recently become more popular. We present the case of a 73-year-old female patient with neck pain radiating to right shoulder and arm. The patient had adjacent-level cervical foraminal stenosis at C5–6 and C6–7 on right side and was managed with modified fully-endoscopic posterior foraminotomy at C5–6 and C6–7 with total pediculectomy of C6. The patient exhibited excellent clinical results, without any instability during long-term follow-up. Fully-endoscopic posterior cervical 2-level foraminotomy using total pediculectomy can be applied in patients for whom the anterior cervical approach is contraindicated, with the added advantages of the minimally invasive posterior approach. The technique has an extensive learning curve and needs to be selected according to the pathology.
Outer inverses in semigroups belonging to the prescribed Green’s equivalence classes
Authors: Ćirić Miroslav , Ignjatović Jelena , Stanimirović Predrag
Published: July 2023

We provide existence criteria and characterizations for outer inverses in a semigroup belonging to the prescribed Green’s ℛ-, ℒ- and ℋ-classes. These results generalize the well-known problem of finding outer inverses of a matrix over a field with the prescribed range or/and null space. Outer inverses belonging to the prescribed Green’s ℛ- and ℒ-classes also represent extensions of Drazin’s (b, c)-inverses and Mary’s inverses along an element. We provide an overview of other such extensions that have emerged recently and compare them with the extensions introduced in this paper.

Mathematics Subject Classification (2010). 20M99, 15A09, 15A24.
“It's a habit. They've been doing it for decades and they feel good and safe.”: A qualitative study of barriers and opportunities to changing antimicrobial use in the Indonesian poultry sector
Authors: Hibbard Rebecca , Chapot Lorraine , Yusuf Havan , Ariyanto Kurnia Bagus , Maulana Kusnul Yuli , Febriyani Widya , Cameron Angus , Vergne Timothée , Faverjon Céline , Paul Mathilde C.
Published: July 2023

Interventions to change antimicrobial use (AMU) practices can help mitigate the risk of antimicrobial resistance (AMR) development. However, changing AMU practices can be challenging due to the complex nature of the factors influencing AMU-related behaviours. This study used a qualitative approach to explore the factors that influenced decision-making on AMU by farmers and other actors in the Indonesian poultry sector. Thirty-five semi-structured interviews were conducted with farmers, technical services staff from the private sector, and representatives of associations, universities, and international organisations in Central Java, West Java, and East Java. Thematic analysis identified three patterns of influence on AMU: how farmers used information to make AMU-related decisions, the importance of farmers’ social and advisory networks, and the motivations driving changes in AMU behaviours. Key barriers identified included a lack of shared understanding around when to use antibiotics, financial pressures in the poultry sector, and a lack of engagement with government veterinary services. Potential opportunities identified included high farmer awareness of AMU, identification of private sector actors and peer networks as the stakeholders with established relationships of trust with farmers, and the importance of farmers’ conceptions of good farming practices, which could be engaged with to improve AMU practices.
​A Three-Dimensional DV-Hop Algorithm Based on the Improved Grey Wolf Algorithm for Wireless Sensor Networks
Authors: Wu YU Xiu , Shuai YE , Yong LIU
Published: July 2023

Location technology has become one of the core technologies of the Internet of Things, especially for wireless sensor networks. The DV-Hop algorithm is used in various situations as one of the representative algorithms. Aiming at the large positioning error of the DV-Hop algorithm, this paper proposes a three-dimensional DV-Hop algorithm based on the improved Grey Wolf algorithm (TDIGW). First of all, the algorithm refines the number of hops through a multi-communication radius strategy, which reduces errors caused by the number of node hops. Then, mean square error is introduced to optimize the average hop distance of the beacon nodes. Finally, the grey wolf algorithm with dynamic weight factor optimization is used instead of least squares to calculate the coordinate positions of the unknown nodes. The simulation results show that compared with the traditional 3D-DVHop algorithm and other algorithms. the average localization error of this algorithm is reduced by 20.13%, 12.62% and 8.98% respectively. The algorithm proposed in this paper reduces the localization error effectively.
Optimize incremental SFM for repetitive structures and short baseline issues
Authors: Li Bing , Ma Hengtao , Mi Wansheng
Published: July 2023

Structure from Motion has been widely used in 3D reconstruction based on multiview geometry, which is used to construct sparse point clouds and solve camera pose information. However, there are often problematic matching pairs in image sets, which makes it difficult to obtain correct reconstruction results by SFM method. Aiming at the problems of repetitive structure and short baseline, this paper constructs the Minimum Spanning Tree to filter the mismatched image pairs, and reconstructs the connection relationship between images, thus improving the incremental SFM method. In the initialization stage, the edge weights are calculated, and the MST is extracted, and then iterative merging is performed to get the final image connection graph from which the mismatching is filtered, and then it is expanded in an incremental way until the images in the whole data set are traversed. The evaluation results of public data sets and data sets with repeated structures show that the improved incremental SFM method can achieve similar or better reconstruction results.
RETRACTED: Wear Mechanism Analysis of Internal Chip Removal Drill for CFRP Drilling
Authors: Xu Chengyang , Liu Xueqing , Wang Gongdong , Sun Mingliang , Wang Yiwen , He Long
Published: July 2023

The authors have requested that this preprint be removed from Research Square.
Influence of co-ensiled rice straw with whole sugar beet on lactating cows performance
Authors: Abo-Donia Fawzy Mohamed , Elsheikh Hanim , Esh Ayman , El-Shora Mohamed , Eldiahy Yasser
Published: July 2023

This study investigated the effect of co-ensiled rice straw (RS) with whole sugar beet (SB) on lactating cows’ performance. Ensiled rice straw as control (SCG) was incorporated with immersed corn grains (CG) for 24 h, while the 2nd and 3rd ensiled RS (LSB and HSB) contained SB substituted of 50 and 100% of CG on an energy basis (total digestible nutrients, TDN), respectively. Ensiled RS were offered ad-libitum while a concentrate feed mixture (2% of body weight) was provided in diets, D1, D2, and D3, respectively. The HSB has a slightly higher population of lactic acid bacteria, consort with LSB and SCG. The OM, CP, EE, NFC, and TCH contents of SCG were slightly higher than LSB or HSB, while the opposite happened with the aNDFom, and ADFom contents. The digestibility of DM, OM, aNDFom, and ADFom of the D3 group was higher (P < 0.05) than in D1 and D2. The D3 recorded the highest values (P < 0.05) of silage consumption, and palatability. Milk production, fat-corrected milk (FCM), and energy-corrected milk (ECM) were (P < 0.05) higher for cows fed D3 compared with D1 and D2. Fat, protein, lactose, and total solids. The feed conversion ratio (FCR) of cows fed diet D3 was better than cows fed D1 diet. Blood constituents remained unchanged and were within the normal ranges. In conclusion, co-ensiling of RS with the whole SB plant consider a good method to improve its nutritional value.
Novel composite membrane based on Recycled low-density polyethylene-alumina used for vacuum membrane distillation
Authors: Ajari Hanen , Khaled Fatma , Akrout Hiba , Khaoula Hidouri , Chaouachi Bechir , Alsalhy Qusay
Published: July 2023

Background In this study, a composite membrane was prepared from recycled low-density polyethylene, thus helping to lighten plastics’ load on the environment, while the powder of alumina was used to enhance the membrane contact angle, its porosity and its mechanical properties. The membrane preparation was made by means of the thermal induced phase separation method by using the butyl acetate as a solvent, hexane as a non-solvent, and the alumina as an additive. Results: The membrane crystalline property was assessed via the Fourier transform infrared spectroscopy. The membrane characteristics were then investigated in terms of thickness, contact angle, pore size, porosity, mechanical test, bubble point pressure, AFM, and SEM analysis. The results revealed that the addition of alumina had an important role in improving membrane structure, properties and therefore its performance. Conclusions The evaluation of the recycled low-density polyethylene-alumina membrane showed that it has a good porosity, good hydrophobicity, and better mechanical properties. The obtained membranes were also applicated to the vacuum membrane distillation to test their performance.
The m6A methyltransferase RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism
Authors: Park Yun-Yong , Ju﻿ Jin-Sung , Park Su Hwan , Yun Hye Jun , Lee Su bin , Kim Seok-Ho , Lee Jong-Ho , Győrffy Balázs , Kim Ho , Yang Siyoung , Eyun Seong-il , Woo Hyun Min
Published: July 2023

N 6 -adenosine methylation (m6A) is critical for controlling cancer cell growth and tumorigenesis. However, the function and detailed mechanism of how m6A methyltransferases modulate m6A levels on specific targets remains unknown. In the current study, we identified significantly elevated levels of RBM15, an m6A writer, in basal-like breast cancer (BC) patients compared to non-basal like BC and linked it to worse clinical outcome. Gene expression profiling uncovered correlations between RBM15 and serine and glycine metabolism genes including PHGDH, PSAT1, PSPH, and SHMT2. RBM15 influences overall m6A levels and, specifically, m6A of serine and glycine metabolism genes via direct binding to target RNA. Further RBM15 effects on cell growth were largely dependent on serine and glycine metabolism. Thus, RBM15 coordinates cancer cell growth through altered serine and glycine metabolism suggesting RBM15 as a new therapeutic target in BC.
Rv2231c, a unique histidinol phosphate aminotransferase from Mycobacterium tuberculosis, supports virulence by inhibiting host-directed defense
Authors: Zarin Sheeba , Shariq Mohd , Rastogi Nilisha , Ahuja Yashika , Manjunath P. , Alam Anwar , Hasnain Seyed Ehtesham , Ehtesham Nasreen Zafar
Published: July 2023

Nitrogen metabolism of M. tuberculosis is critical for its survival in infected host cells. M. tuberculosis has evolved sophisticated strategies to switch between de novo synthesis and uptake of various amino acids from host cells for metabolic demands. Pyridoxal phosphate-dependent histidinol phosphate aminotransferase-HspAT enzyme is critically required for histidine biosynthesis. HspAT is involved in metabolic synthesis of histidine, phenylalanine, tyrosine, tryptophan, and novobiocin. We showed that M. tuberculosis Rv2231c is a conserved enzyme with HspAT activity. Rv2231c is a monomeric globular protein that contains α-helices and β-sheets. It is a secretory and cell wall-localized protein that regulates critical pathogenic attributes. Rv2231c enhances the survival and virulence of recombinant M. smegmatis in infected RAW264.7 macrophage cells. Rv2231c interacts with TLR4 immune receptor and modulates host immune response suppressing the secretion of antibacterial pro-inflammatory IL-12 and IL-6 cytokines. It also inhibits the expression of co-stimulatory molecules CD80 and CD86 along with antigen presenting molecule MHC-I on macrophage and suppresses reactive nitrogen species formation, thereby promoting M2 macrophage polarization. Rv2231c inhibits intrinsic apoptosis while activating necroptosis and pyroptosis, thereby promoting efficient bacterial survival and proliferation to increase virulence. Our results indicate that Rv2231c is a moonlighting protein that regulates multiple functions of M. tuberculosis pathophysiology to increase its virulence. These mechanistic insights can be used to better understand the pathogenesis of M. tuberculosis and to design strategies for tuberculosis mitigation.
Aedes aegypti midgut microbiota establishment in adult females is regulated by Juvenile Hormone to enhance fecundity and fitness.
Authors: Taracena Mabel , Walter-Nuno Ana , Bottino-Rojas Vanessa , Dotson Ellen , Oliveira Pedro , Paiva-Silva Gabriela O.
Published: July 2023

Understanding the physiological phenomena involved in female mosquitoes' preparation for a blood meal, including the effects of mating, is crucial for enhancing vector-control strategies against mosquito-borne diseases. At the same time, the mosquitoes’vector capacity can be impacted by fecundity and longevity, making important the study of the factors that can influence these. Our findings reveal that mating and Juvenile Hormone induce midgut growth in Aedes aegypti mosquitoes. Additionally, we observed the gradual establishment of a native bacterial population in the mosquito midgut due to JH-dependent suppression of the immune response. Mated mosquitoes and those treated with JH exhibited lower expression levels of Anti-Microbial Peptides. The increased presence of bacteria in the midgut stimulated epithelial cell turnover, indicating the dynamic response of the epithelium to the microbiota. Here we demonstrate how mating and JH levels can influence organ size and immunity in the mosquito midgut. JH-induced expansion of the microbiota downregulated certain antimicrobial peptides in Ae. aegypti, leading to increased egg counts and longer lifespans. Overall, these findings provide evidence of a cross-talk between JH response, gut epithelial tissue, cell cycle regulation, and the mechanisms governing the trade-offs between nutrition, immunity, and reproduction at the cellular level in the mosquito gut.
Assessing peri-implant bacterial community structure: the effect of microbiome sample collection method
Authors: Anitua Eduardo , Murias-Freijo Alia , Tierno Roberto , Tejero Ricardo , Alkhraisat Mohammad Hamdan
Published: July 2023

The Objective:
The aim of this study was to evaluate the effect of different oral sampling approaches on metagenomic profiles of peri-implant bacterial communities as revealed by 16S rRNA sequencing.
Background data:
The oral cavity is an exceptionally complex habitat that harbors a large number of microorganisms. Oral microbiota comprises polymicrobial communities shaped by mutualistic coevolution with the host, contributing to homeostasis and regulating immune function. Nevertheless, dysbiosis of oral bacterial communities is associated with a number of clinical symptoms that ranges from infections to oral cancer. Peri-implant diseases are biofilm-associated inflammatory conditions affecting the soft and hard tissues around dental implants. Therefore, the detection of early symptoms of infection are essential to prevent disease progression and implant failure. Since oral microbiota is also strongly associated with peri-implant health status, the study of oral bacterial communities may be a promising approach for monitoring peri-implant health.
Methods:
With the aim of selecting an appropriate microbiome sampling procedure, next generation sequencing was used for characterizing the bacterial communities of three sample types two months after transepithelial abutment placement: adjacent periodontal crevicular fluid (ToCF), crevicular fluid from transepithelial abutment (TACF) and transepithelial abutment (TA).
Results:
Significant differences in multiple alpha diversity indices were detected at both the OTU and the genus level between different sampling procedures. Differentially abundant taxa were detected between sample collection strategies, including peri-implant health and disease related taxa. At the community level significant differences were also detected between TACF and TA and also between TA and ToCF. Moreover, differential network properties and association patterns were identified.
Conclusion:
These results suggest that microbiome profiles could assist peri-implant health surveillance. However, the selection of sample collection strategy can significantly affect the community composition and structure.
HPAIV outbreak triggers enhanced colony connectivity in a seabird metapopulation
Authors: Jeglinski Jana Wibke Elisabeth , Lane Jude , Votier Stephen C , Furness Robert W , Hamer Keith C , McCafferty Dominic , Nager Ruedi G , Sheddan Maggie , Wanless Sarah , Matthiopoulos Jason
Published: July 2023

Disease outbreaks can drastically disturb the environment of surviving animals, but the behavioural, ecological, and epidemiological consequences of disease-driven disturbance are poorly understood. Here, we show that an outbreak of high pathogenicity avian influenza (HPAI) coincided with unprecedented short-term behavioural changes in Northern gannets (Morus bassanus). Breeding gannets have been characterised by strong fidelity to their nest sites and foraging areas (2015 – 2019; n = 120), but during the 2022 HPAI outbreak, GPS-tagged gannets instigated long-distance movements and the first ever recorded visits of adults to other gannet breeding colonies. Our findings suggest that the HPAIV outbreak triggered changes in space use patterns of exposed individuals that amplified the epidemiological connectivity among colonies and may generate super-spreader events that accelerate disease transmission across the metapopulation. Such self-propagating transmission from and towards high density animal aggregations may explain the unexpectedly rapid pan-European spread of HPAIV in the gannet.
Severe liver steatosis as potential risk factor of cardiovascular disease in patients with type 2 diabetes: a prospective cohort study
Authors: Firouzabadi Mohammad Dehghani , Poopak Amirhossein , Sheikhy Ali , Firouzabadi Fatemeh Dehghani , Moosaie Fatemeh , Rabizadeh Soghra , Momtazmanesh Sara , Nakhjavani Manouchehr , Esteghamati Alireza
Published: July 2023

Background and Aims: Non-alcoholic fatty liver disease (NAFLD), accelerates the progression of atherosclerosis and development of cardiovascular diseases (CVD) which is the most common cause of mortality in NAFLD patients. Methods and results In this prospective cohort study, 1197 patients with type 2 diabetes (T2D) were divided in to two groups (360 patients with NAFLD and 847 without) and were followed for median of 5 years for incidence of CVD. Cox regression analysis was used to assess the association between NAFLD, level of liver enzymes, and aspartate aminotransferase to platelet ratio index (APRI), and incidence risk of CVD and its subgroups (i.e., Myocardial Infarction, chronic heart disease, coronary artery bypass grafting, and percutaneous coronary intervention. There was a significant positive association between CVD incidence and NAFLD (HR = 1.487, 95% CI = 1.041–2.124, p-value = 0.029). Although patients with NAFLD had higher ALT and AST levels (p-value = < 0.001), there was no significant association between liver enzymes and the incidence risk of CVD when adjusting for different variables. Furthermore, NAFLD was linked to APRI Q (2), APRI Q(3), and APRIQ(4) NAFLD (1.365 (1.046–1.781), 1.623 (1.234–2.135), and 3.373 (2.509–4.536), respectively. Conclusion NAFLD increased the incidence risk of CVD in T2D. However, there was no association between liver enzymes (ALT, AST, ALK-P, and GGT) and a higher incidence risk of CVD in T2D when adjusted for confounding variables.
The impact of COVID-19 on routine child immunisation in South Africa
Authors: Moyo Sangiwe , Ashok Anushka , Sharma Saransh , Nyankieya Rebecca , Prasad Ram , Myers Laura
Published: July 2023

Background
The COVID-19 pandemic disrupted immunisation programs worldwide, reversing gains that had brought vaccine-preventable diseases largely under control. This study explored the impact of COVID-19 on the uptake of routine child immunisation services in South Africa.
Methods
We conducted qualitative research using in-depth interviews with 51 purposively selected parents/caregivers of children below the age of five who missed or delayed one or more scheduled immunisation doses in 2020-2022 and with 12 healthcare providers who provided public immunisation services during the pandemic.
Findings
During the pandemic lockdowns, most caregivers perceived the risk of their child being infected with COVID-19 during a clinic visit as more salient than the risk of missing immunisation doses. Caregivers reported minimal exposure to routine immunisation communication, as well as shortages of routine vaccines for children at public health facilities; healthcare workers experienced anxiety and burnout. There was a post-pandemic shift to more active decision-making about immunisation, which had previously been an almost automatic behaviour, leading some caregivers to delay vaccinating their children. There was also evidence of a “bad vaccine” mental model among some caregivers regarding COVID vaccinations, which could lead to doubts about the safety of routine childhood vaccinations.
Interpretation
The shift from default to active decision-making highlights a risk that routine immunisation will backslide in future pandemics. Governments should build resilient health systems at all levels and communicate clearly about the benefits and availability of RIs and the safety of vaccinations in general, alongside supply-side interventions.
Funding
Final Mile Consulting funded this study.
Implementation of policy and management interventions to improve health and care workforce capacity to address the COVID-19 pandemic response: a systematic review
Authors: de Oliveira Ana Paula Cavalcante , Galante Mariana Lopes , Maia Leila Senna , Craveiro Isabel , Silva Alessandra Pereira da , Fronteira Inês , Chança Raphael , Cometto Giorgio , Ferrinho Paulo , Poz Mario Roberto Dal
Published: July 2023

BACKGROUND
The COVID-19 pandemic highlighted pre-existing weaknesses in health and care systems and services and shortages of health and care workers (HCWs). As a result, policymakers needed to adopt measures to improve the health and care workforce (HCWF) capacity. This review aims to identify countries’ range of policies and management interventions implemented to improve HCWs’ capacity to address the COVID-19 pandemic response, synthesize their evidence on effectiveness, and identify gaps in the evidence.
METHODS
The literature was searched in PubMed, Embase, Scopus, LILACS-BVS, WHO’s COVID-19 Research Database and the ILO, OECD and HSRM websites for literature and documents published between January 2020 and March 2022. Eligibility criteria were HCWs as participants and policy and management interventions aiming to improve HCWF capacity to address the COVID-19 pandemic response. Risk of bias was assessed with Joanna Briggs Institute (JBI) Critical Appraisal Tools (CAT) and certainty of the evidence in presented outcomes with GRADE.
RESULTS
The searches retrieved 3378 documents. A total of 69 were included, but only 8 presented outcomes of interventions implemented. Most of the selected documents described at least one intervention implemented by countries at the organizational environment level to increase the flexibility and capacity of the HCWF to respond to the pandemic, followed by interventions to attract and retain HCWs in safe and decent working environments. There was a lack of studies addressing social protection, human resources for health information systems, and regarding the role of community health workers and other community-based providers. Regarding the risk of bias, most of documents were rated as medium or high quality (JBI’s CAT), while the evidence presented for the outcomes of interventions was classified as mostly low-certainty evidence (GRADE).
CONCLUSION
Countries have implemented various interventions, some innovative, in response to the pandemic, and others had their processes started earlier and accelerated by the pandemic. The evidence regarding the impact and efficacy of the strategies used by countries during the pandemic still requires further research.
Deep-Learning-Based Natural-Language-Processing Models to Identify Cardiovascular Disease Hospitalisations of Diabetic Patients from Routine Visits’ Text
Authors: Guazzo Alessandro , Longato Enrico , Fadini Gian Paolo , Morieri Mario Luca , Sparacino Giovanni , Camillo Barbara Di
Published: July 2023

Writing notes is the most widespread method to report clinical events. Therefore, most of the information about the disease history of a patient remains locked behind free-form text. Natural language processing (NLP) provides a solution to automatically transform free-form text into structured data. In the present work, electronic healthcare records data of diabetic patients were used to develop deep-learning based NLP models to automatically identify, within free-form text describing routine visits, the occurrence of hospitalisations related to cardiovascular disease (CVDs), an outcome of diabetes. Four possible time windows of increasing level of expected difficulty were considered: infinite, 24 months, 12 months, and 6 months. Model performance was evaluated by means of the area under the precision recall curve, as well as precision, recall, and F1-score after thresholding. Results showed that the proposed NLP approach was successful for both the infinite and 24-month windows, while, as expected, performance deteriorated with shorter time windows. Possible clinical applications of tools based on the proposed NLP approach include the retrospective filling of medical records with respect to a patient’s CVD history for epidemiological and research purposes as well as for clinical decision making.
Identification and verification of genes associated with hypoxia microenvironment in Alzheimer's disease
Authors: Yuan Mingyang , Feng Yanjin , Zhao Mingri , Xu Ting , Li Liuhong , Guo Ke , Hou Deren
Published: July 2023

As the incidence of Alzheimer's disease (AD) increases year by year, more people begin to study this disease. In recent years, many studies on reactive oxygen species (ROS), neuroinflammation, autophagy, and other fields have confirmed that hypoxia is closely related to AD. However, no researchers have used bioinformatics methods to study the relationship between AD and hypoxia. Therefore, our study aimed to screen the role of hypoxia-related genes in AD and clarify their diagnostic significance. A total of 7681 differentially expressed genes (DEGs) were identified in GSE33000 by differential expression analysis and cluster analysis. Weighted gene co-expression network analysis (WGCNA) was used to detect 9 modules and 205 hub genes with high correlation coefficients. Next, machine learning algorithms were applied to 205 hub genes and four key genes were selected. Through the verification of external dataset and quantitative real-time PCR (qRT-PCR), the AD diagnostic model was established by ANTXR2, BDNF and NFKBIA. The bioinformatics analysis results suggest that hypoxia-related genes may increase the risk of AD., However, more in-depth studies are still needed to investigate their association, This article would guide the insights and directions for further research.