bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Relationship between ADHD and Comorbid Psychiatric Conditions: A Comprehensive Study on a Large Cohort
Authors: Sarwar Adeel , Vitetta Frank
Published: November 2023

This study investigates the prevalence and nature of psychiatric comorbidities in individuals diagnosed with attention-deficit/hyperactivity disorder (ADHD), with a focus on diverse gender categories including transgender and non-binary individuals. Utilizing data from 1,528 participants sourced from the ADHDtest.ai website and under the guidance of MD Adeel Sarwar, employed standardised diagnostic tools to identify ADHD and subsequent comorbid psychiatric conditions. This investigation highlights a pronounced prevalence of anxiety, particularly among non-binary (63.2%) and transgender individuals (37.5%), suggesting the need for specialized care. Additionally, we observed a substantial number of females diagnosed with ADHD (44.97%) didn’t report any pre-existing conditions, a distinctive manifestation of ADHD within this demographic group. The discussion emphasizes the intertwined nature of ADHD with other conditions and the importance of gender-specific considerations in diagnosis and treatment. The study conclusion aligns with the National Institute of Mental Health's findings on ADHD comorbidity, underscoring the imperative for a multifaceted evaluative approach to improve clinical outcomes. This research enriches the comprehension of the intricate psychiatric terrain associated with ADHD, highlighting the imperative for ongoing investigations into its concurrent disorders and the repercussions these have on tailored therapeutic approaches.
Detection of Alport gene variants in children and young people with persistent haematuria
Authors: Ng Natasha Su Lynn , Yamamura Tomohiko , Shenoy Mohan , Stuart Helen M , Lennon Rachel
Published: November 2023

Background
Genetic kidney disease is an important cause of persistent microscopic haematuria in children and young people. We aimed to determine the frequency of variants in the Alport syndrome genes (COL4A3, COL4A4 or COL4A5) in individuals under 18 years of age presenting with persistent microscopic haematuria to a single specialist centre in the United Kingdom over a 10-year period.
Methods
We conducted a retrospective longitudinal study of individuals referred to a tertiary paediatric nephrology service with persistent microscopic haematuria between April 2012 to 2022.
Results
224 individuals (female 51.8%) were evaluated with persistent microscopic haematuria of greater than 6 months duration. The age at presentation was 7.5±4.3 years (mean±SD). Targeted exome sequencing was performed in 134 with 91 individuals (68%) identified to have a pathogenic or likely pathogenic variant in COL4A3, COL4A4 or COL4A5. Only 49.5% of individuals with identified variants had a family history of microscopic haematuria documented and 57.1% had additional proteinuria. COL4A5 was the commonest gene affected and missense variants affecting glycine residues were the most common variant type.
Conclusion
Over two thirds of children and young people who underwent genetic testing had an identifiable genetic basis for their microscopic haematuria and over half did not have a documented family history. Genetic testing should be part of the evaluation of persistent microscopic haematuria despite a negative family history.
Enhanced quantum coherence of plasmonic resonances with a chiral exceptional point
Authors: Yuwei Lu , Liu Jing-Feng , Liu Renming , Jiang Haoxiang
Published: November 2023

Strong dissipation of plasmonic resonances is detrimental to quantum manipulation. In this work, we showcase the quantum coherence of plasmonic resonances can be significantly enhanced by integrating with photonic cavity operated at a chiral exceptional point (CEP), where the phase of light offers a new degree of freedom for flexibly manipulating quantum dynamics. The few-mode quantization theory is employed to demonstrate the advantages and related quantum-optics applications of the proposed hybrid cavity in both off- and on-resonance plasmon-photon coupling. For the former case, local density of states (LDOS) can evolve into sub-Lorentzian lineshape, with order-of-magnitude enhancement and linewidth narrowing compared to that without CEP. This results in the enhanced coherent light-matter interaction accompanied by reduced dissipation of polaritonic states. On resonance two mechanisms are responsible for significant improvement of quantum yield at CEP, by reducing plasmonic absorption through Fano interference and enhancing cavity radiation through superscattering. The latter allows achieving near-unity quantum yield in cryostat and enhancing room-temperature quantum yield by two orders of magnitude. Therefore, CEP-engineered plasmonic resonances can serve as a promising platform for exploring the quantum states control by virtue of the non-Hermiticity of open resonators and building high-performance quantum devices for sensing, spectroscopy, and quantum computing.
Implementation of a Crisis Resolution Team service improvement programme: A qualitative study of the critical ingredients for success
Authors: Lamb Danielle , Milton Alyssa , Forsyth Rebecca , Lloyd-Evans Brynmor , Akther Syeda , Fullarton Kate , O'Hanlon Puffin , Johnson Sonia , Morant Nicola
Published: November 2023

Background
Crisis Resolution Teams (CRTs) offer home-based care for people in mental health crisis, as an alternative to hospital admission. The success of CRTs in England has been variable. In response to this, the CRT Optimization and RElapse prevention (CORE) study developed and trialled a 12-month Service Improvement Programme (SIP) based on a fidelity model. This paper describes a qualitative evaluation of the perspectives of CRT staff, managers, and programme facilitators. We identify barriers and facilitators to implementation, and mechanisms by which service improvements took place.
Methods
Managers and staff from six purposively sampled CRTs were interviewed, as well as six facilitators who were employed to support the implementation of service improvement plans. Semi-structured focus groups and individual interviews were conducted and analysed using thematic analysis.
Results
A majority of participants viewed all components of the SIP as helpful in improving practice, although online resources were under-used. Perceived barriers to implementation centred principally around lack of staff time and ownership. Support from both senior staff and facilitators was essential in enabling teams to undertake the work associated with the SIP. All stakeholder groups reported that using the fidelity model to benchmark their CRT work to best practice and feel part of a ‘bigger whole’ was valuable.
Conclusion
CRT staff, managers and programme facilitators thought that a structured service improvement programme helped to increase fidelity to a best practice model. Flexibility (from all stakeholders) was key to enable service improvement actions to be manageable within time- and resource-poor teams.
The landscape of Hepatocellular Carcinoma and Tumor-associated Telocytes Integrated Single-nucleus RNA Sequence and Transcriptomics Technology
Authors: Xu Ying , Li Mengyu , Zhang Jinqing , Ding Ning
Published: November 2023

Background: Hepatocellular carcinoma (HCC) is the main malignant tumor in humans, known for its high metastasis and lethality. However, the molecular mechanisms underlying tumorigenesis in HCC are still unclear.
Methods: To address this, we conducted a comprehensive analysis using single nucleus RNA sequencing (snRNA-seq) assay and transcriptomic technology. This approach allowed us to uncover genetic changes in cancer cells and other cells within the tumor microenvironment, leading to the identification of potential hub genes for HCC.
Results: Our study analyzed snRNA-seq and transcriptomics data, revealing novel differentially expressed genes (DEGs) in HCC and identifying characteristic biomarkers for telocytes (TCs) through bioinformatics analysis. Notably, STAB2, OIT3, BMPER, MARCO, CRHBP, and FCN2 showed high specificity and sensitivity in predicting the prognosis or development of HCC.
Conclusion: TMC5 and TRABD2B were identified as unique biomarkers for TCs in the liver. These DEGs warrant further investigation to validate their potential implications in HCC.
Assessment of American Bullfrog (Lithobates catesbeianus) spreading in the Republic of Korea using rule learning of elementary cellular automata
Authors: Oh Gyujin , Wi Yunju , Kang Hee-Jin , Cheon Seung-ju , Sung Ha-Cheol , Kim Yena , Jin Hong Sung
Published: November 2023

The spread of American Bullfrog, one of the 100 of the World’s Worst Invasive Alien Species, has a great impact on the surrounding ecosystem. Little is known about the tendancy and pattern of how they are spreading in South Korea geographically. It is important to study the tendancy of their spreading so that a proper mitigation can be applied when needed. This study is based on the results of national surveys that observed the distribution. The entire data is divided into 25 regional clusters using the divisive hierarchical clustering method. In order to estimate the degree of spreading, a sequence of spatial distribution is constructed for each cluster using the agglomerative clustering method. ECA(elementary cellular automata) is introduced to find rules governing the pattern variation in the sequence. Each cell represents either the observed or unobserved site of bullfrog. The number of Bullfrog Observed Site (BOS) in a sequence of each cluster is counted and used to define the spreading intensity. The rules of ECA are trained and learned by the CNN(Convolution Neural Network) method and used to estimate and predict the spreading intensity by counting the number of BOS. Taking environmental factors into account, habitat suitability is used and obtained using Maxent. The spreading intensity is multiplied by the habitat suitability to get an assessment of bullfrogs spreading. The relative spreading assessment is estimated, which is classified into 4 groups; spreading intensively, spreading slowly, maintaining or declining population.
Metabolomic and Transcriptomic Analyses Reveals Candidate Genes and Pathways Involved in Secondary Metabolism in Bergenia purpurascens
Authors: Yu Fang , Zhang Xuebin , Xu Nuomei , Chen Jingyu , Lyu Xin , Wu Yufeng , Zeng Hongyan , Zhu Qiankun
Published: November 2023

Bergenia purpurascens is an important medicinal, edible and ornamental plant. The lack of omics information hinders the study of its metabolic pathways and related genes. In order to investigate candidate genes and pathways involved in secondary metabolism in B. purpurascens, roots, stems and leaves of B. purpurascens were subjected to metabolomic and transcriptomic analyses in this study. A total of 351 differentially accumulated secondary metabolites were identified. We identified 111 candidate enzyme genes in phenylpropanoid and flavonoid biosynthesis pathway. 22 candidate key genes related to phenylpropanoid and flavonoid synthesis were obtained by WGCNA analysis, from which 14 structural genes in phenylpropanoid and flavonoid synthesis and five transcription factors related to flavonoid synthesis were identified. This study provides data resources and new insights for further studies on the biosynthesis of major active components in B. purpurascens.
Sources of resistance to Fusarium oxysporum f. sp. vanillae in Vanilla spp
Authors: Bautista-Aguilar José R , Iglesias-Andreu Lourdes G. , Luna‑Rodríguez Mauricio , Sánchez-Coello Nadia G
Published: November 2023

Fusarium oxysporum f. sp. vanillae (Fov) is the most devastating pathogen of the vanilla crop. A possible solution to this phytosanitary problem is to obtain promising genotypes with some degree of resistance to Fov. The use of biotechnological tools has been very useful for this purpose, so the present study was proposed to evaluate the degree of resistance to Fov in four accessions of Vanilla: Vanilla F1 hybrid (V. planifolia x V. pompona), V. planifolia "RH350" (V. planifolia resistant to Fov strain H3), V. planifolia "La Gran Lucha" (LGL, wild morphotype from Oaxaca, Mexico), and V. planifolia "Mansa" (commercial morphotype from Veracruz, Mexico). For this purpose, the mean lethal concentration (LC50) of the culture filtrates (FC) obtained from the pathogenic strain M21C5 of Fov was first determined based on the survival of V. planifolia shoots. The LC50 value for the Fov (strain evaluated) was determined to be 35%. Differences were found in the survival of in vitro seedlings subjected to 40% of the Fov culture filtrates. The highest survival values (75% and 70%) were found in V. planifolia "RH350" and V. planifolia "La Gran Lucha" accessions, respectively. In vivo tests under greenhouse conditions indicated that V. planifolia "RH350" and V. planifolia "La Gran Lucha" showed high values of survival (44± 2.6 and 37± 2.2, respectively) and Fov resistance (36±2.1 and 30±1.8, respectively). The results obtained in this study are of great interest for the development of genetic improvement work currently being developed for this crop.
Streamlined Biotinylation, Enrichment and Analysis for Enhanced Plasma Membrane Protein Identification Using Turboid and Turboid-Start Biotin Ligases
Authors: SARIHAN MEHMET , KASAP MURAT , AKPINAR GURLER
Published: November 2023

Plasma membrane proteins play pivotal roles in various cellular events and are crucial in disease pathogenesis, making their comprehensive characterization vital for biomedical research. However, the hydrophobic nature and low expression levels of PMPs pose challenges for conventional enrichment methods, hindering their identification and functional profiling. In this study, we presented a novel TurboID-based enrichment approach for PMPs that helped overcoming some of the existing limitations. We evaluated the efficacy of TurboID and its modified form, TurboID-START, in PMP enrichment, achieving more efficient and targeted labeling of PMPs without the need for stable cell line generation. This approach significantly reduced non-specific biotinylation events, leading to improved PMP enrichment and enabling a precise assessment of the subcellular proteome associated with the plasma membrane. Our findings highlighted the dynamic nature of the plasma membrane as well and captured transient associations of proteins with the plasma membrane. The novel TurboID-based enrichment approach presented here offers promising prospects for in-depth investigations into PMPs and their roles in cellular processes.
Metal-metal attachment of absorbers for Gamma-Ray TES
Authors: Dasgupta Adrika , Becker Daniel , Bennett Douglas , Keller Mark , Kislat Fabian , Schmidt Daniel , Swetz Daniel , Weber Joel , Wimalasena Kasun
Published: November 2023

In this work, we are reporting on the development of a method to attach Sn absorbers to the Transition Edge Sensors (TESs) with BiSn spheres (metal-to-metal attachment), using a die bonder, with the aim of improving thermalization, potentially reducing the presence of an a-thermal component in the tails of signal pulses. We describe our efforts towards finding optimal temperature and pressure conditions for making this contact and the progress toward contact resistance measurements of these joints.
The correlation between levels of serum neurofilaments light and cognitive performance in older adults within the United States
Authors: Xue Kun , Xing Shanshan
Published: November 2023

INTRODUCTION:
The aim of this study was to ascertain the association between sNfL levels and cognitive function.
METHODS:
In order to perform our analysis, we used National Health and Nutrition Examination Survey (NHANES) data from 2013 to 2014. 502 elderly persons (older than 60) were the subject of our investigation. Using a variety of measures, including the CERAD Word List Learning Test (WLLT), Word List Recall Test (WLRT), Intrusion Word Count measures (WLLT-IC and WLRT-IC), Animal Fluency Test (AFT), and Digit Symbol Substitution Test (DSST), the main goal was to assess cognitive performance. We utilized multiple linear regression and cubic spline analyses, correcting for a number of factors.
RESULTS:
The inverse association between sNfL and DSST was found to be statistically significant (β: -2.3, p = .045), indicating a detrimental impact on the DSST score. There was no indication that this connection had a plateau effect.
CONCLUSIONS:
There was a notable inverse correlation observed between sNfL levels and processing speed, sustained attention, and working memory in older adults aged 60 years and above.
Co-infection of Solanum tuberosum with PVX and PVY leads to systemic virus-mediated gene expression
Authors: Mehdi Rabih , Eydam Julia , Sonnewald Uwe
Published: November 2023

Background
Breeding novel crop cultivars can be an extremely laborious and time-consuming process, especially when it comes to highly heterozygous and tetraploid potato (Solanum tuberosum L.). Establishing biotechnological techniques to understand gene functions and to accelerate breeding processes are therefore inevitable to sustain resilient potato cultivars for future food security. Apart from being pathogens, viruses represent suitable vectors for delivering genetic information into plant cells. Taking regulatory obstacles for genetically modified crops into account, RNA viruses provide a convenient tool to introduce desired RNA molecules into plants without the application of foreign DNA. Hence, developing an RNA virus-mediated gene expression system to study and improve crop plants is of great importance. Additionally, plant RNA viruses represent an opportunity to obtain modified crops without the necessity for tissue culture. With this work, we seek to establish a virus-mediated gene expression system for cultivated potato.
Results
Irrespective of reports claiming successful gene expression or silencing upon infection of potato (S. tuberosum) with RNA viruses, such as potato virus X (PVX), single Agrobacterium-mediated infections with PVX did not lead to a sufficient systemic infection and concomitant expression of a green fluorescent protein (GFP) in various potato cultivars. Using the tuber-bearing species Solanum pinnatisectum for infections, systemic movement of PVX-GFP was detectable throughout the diploid wild potato, pointing out limitations for application in tetraploid potato cultivars. Further, hetero-graftings with Nicotiana benthamiana and S. tuberosum indicated that PVX-GFP can move through the vasculature of potato without leaving the transport tissue. Nonetheless, coinfection of PVX-GFP and potato virus Y (PVY) enabled adequate systemic spreading accompanied by GFP expression in different potato cultivars.
Conclusion
PVX-mediated gene expression is limited in cultivated potato. Utilizing PVY for co-infections facilitates systemic PVX spreading and accompanying expression of a desired gene. This method allows fast and uncomplicated viral delivery of genetic information into S. tuberosum opening up opportunities not only to express desired proteins but also to silence genes of interest and thereby establish epigenetic modifications serving as tool for basic science and possibly for plant breeding purposes.
Effect of Educational Campaign on Interpersonal Communication Skills among Health Care Professionals: Pre-post study, Jeddah, KSA
Authors: Mahmoud Manal Ibrahim Hanafi , Badawey Jude Tareq , Kouki Ahmad Mohamed Khaled , Malik Louay Fadi , Shakally Salman Mohamed Samih , Nasser Asmaa Abdel
Published: November 2023

Background
Communication among healthcare team members is crucial for effective patient care. It helps ensure that everyone involved in the healthcare process is aware of the patient’s needs and treatment plan, which increases the likelihood of positive outcomes and reduces the risk of medical errors. Strong communication can help build trust and collaboration among healthcare team members, which can improve job satisfaction and lead to better patient outcomes.
Aim
To study and explore the effect of an educational campaign on interpersonal communication skills among healthcare professionals in Jeddah, KSA.
Methods
A quasi-experimental pre- and post- study was carried out on a convenient sample of 150 healthcare professionals. A specially prepared self-administered, pre-coded, closed-ended, pilot-tested, reliable questionnaire format was used. Self- assessment communication skills inventory questionnaires were completed initially in the pre-study phase and at the end of a specifically arranged educational campaign as the post-study phase. To control for potential confounding factors, multivariate analyses using a forward stepwise (Wald) method were carried out to determine independent predictors of interpersonal communication skills among the healthcare professionals.
Results
There was a significant improvement of the measured responses to the interpersonal communication skill inventory in all its domains, namely: sending a clear message (p = 0.000), listening (p = 0.000), giving and receiving feedback (p = 0.000) and handling emotional reactions (p = 0.000). Significant independent predictors of interpersonal communication skills were nationality, mother language, specialty, qualification, daily working hours, number of monthly shifts and perceived satisfaction with the work environment. The mean evaluation of the whole campaign out of 10 was 9.6 (4.85), with a mean percent score for evaluation of 77.6 (22.15).
Conclusion
these educational campaigns for the development of interpersonal communication skills have desirable effects on healthcare professionals’ communication abilities and capabilities. Healthcare organizations should invest in training programs that develop healthcare professionals’ communication skills to improve healthcare quality and patient safety.
The Expression of VDBP in the Severe COVID-19 and its Role in Acute Lung Injury
Authors: Jiang Hongjuan , Chi Xiangyu , sun Yanhong , Wang Jing , Li Hongwen
Published: November 2023

Background
Vitamin D-binding protein (VDBP) may be involved in the occurrence and development of COVID-19. This study intends to investigate the expression of VDBP in COVID-19 and to explore the role of VDBP through animal and cellular models of acute lung injury (ALI).
Methods
The serum of COVID-19 and common pneumonia over 65 years old in the Department of Geriatrics of the Shandong Provincial Hospital Affiliated to Shandong First Medical University were collected from 1 December 2022 to 30 July 2023. ELISA was used to detect the expression of serum VDBP. The mouse model of ALI was established by intraperitoneal injection of LPS, and the expression of VDBP in the lung tissue of ALI mice was detected by immunohistochemistry and Western blot. Construct a VDBP gene-silencing plasmid and transfect it into human alveolar epithelial A549 cells. After 72 hours of LPS intervention, collect cells and cell supernatant for testing. CCK8 detection of cell proliferation. Flow cytometry was used to detect apoptosis in cells.
Results
The VDBP of severe COVID-19 was higher than that of non-severe COVID-19 and common pneumonia (p < 0.05), but the VDBP was not an independent risk factor for the severity of COVID-19 (p > 0.05). Immunohistochemistry and Western blot suggest that VDBP in lung tissue of ALI mice was significant overexpression (p < 0.05). VDBP in LPS-induced injury of alveolar epithelial cells was significant overexpression (p < 0.05). The ELISA results showed that after LPS intervention, the inflammatory factor IL-1β and TNF-a was significantly reduced in the VDBP gene silencing group compared to the control group (p < 0.05). The cell proliferation ability of the VDBP gene silencing group was significantly increased compared to the control group, and the cell apoptosis rate was significantly reduced compared to the control group (p < 0.05).
Conclusion
The level of VDBP in severe COVID-19 was significantly increased. VDBP may play an inflammatory factor in ALI and promote apoptosis of bronchial epithelial cells.
The effect of asymmetric regulation on market competition: the case of the 2013 Telecomm Reform in Mexico
Authors: Silva Rodrigo Alcázar , Pastrana Julio Alberto Ramos
Published: November 2023

We examine the economic effects of the 2013 Telecommunications Reform in Mexico that implemented asymmetric regulation on the largest telecommunications operator in the country. This regulation imposed on the “preponderant” telecommunications operator, defined as the operator with more than 50% of the connections of all the sector, a series of requirements aimed to increase market competition and facilitate entry in the sector. We investigate the effects of this regulation on market competition using quarterly data from the Mexican telecommunications operators in the period 2010.3-2017.3. Results provide evidence that the asymmetric regulations fostered competition in two ways: they fostered entry into the sector, which resulted in a decrease of the market share of the preponderant agent, driven by a decrease in the market share of prepaid lines; and at the same time intensified competition by increasing the quality of the services provided by the preponderant agent, which resulted in a net gain in the number of lines transferred to this agent, and an increase in the number of postpaid lines.
Effects of Glaucoma Treatment on Ocular Surface and Tear Functions: Comparison of Trabeculectomy and Antiglaucomatous Drops
Authors: Mermer Abdussamet , Dursun Özer , Bucak Öznur , Sayar Hamide , Bektaş Fatma Merve , Eröz Pınar , Yılmaz Ayça
Published: November 2023

Aim
To investigate the tear function and ocular surface disease findings in glaucoma patients who received antiglaucomatous medication in one eye and trabeculectomy surgery in the other eye.
Methods
The patient group included 38 eyes of 19 patients who underwent trabeculectomy surgery with mitomycin C treatment in one eye at least 6 months ago and were followed up without medication, while the other eye was receiving antiglaucomatous medication. As a control group, 20 eyes of 20 healthy individuals were included in the study. Demographic data, follow-up period after trabeculectomy, antiglaucomatous medications, number of drops per day and duration of medication were recorded. Tear break-up time, corneal and conjunctival fluorescein staining, Schirmer II test and conjunctival impression cytology were performed.
Results
There was a statistically significant difference in tear break-up time, corneal and conjunctival fluorescein staining, Schirmer II test and Nelson staging levels in both eyes of glaucoma patients when compared with the control group (p0.05). Although not statistically significant, tear break-up time, Schirmer II test, punctate staining and Nelson staging results were found to be better with increasing postoperative time.
Conclusions
Medications used in glaucoma patients and trabeculectomy surgery with MMC induced OSD of similar severity in our patient group. There was no superiority between trabeculectomy with MMC and trabeculectomy with MMC in terms of OSD incidence.
(non-aspirin) NSAIDs use and risk of cardiovascular diseases: A Mendelian randomization study
Authors: Liu Guihong , Chen Tao , Zhang Xin , Hu Binbin , Shi Huashan
Published: November 2023

Objectives Non-steroidal anti-inflammatory drugs (NSAIDs) are currently the most common anti-inflammatory and analgesic drugs. Some clinical studies have reported that NSAIDs increase the incidence of several cardiovascular diseases (CVDs). However, a solid causal association has not been demonstrated. In this Mendelian randomization (MR) study, we investigated the causal association of NSAIDs use with the risk of CVDs.Methods A two-sample MR was utilized to determine whether there is a causal relationship between NSAIDs use and the risk of CVDs. Single-nucleotide polymorphisms(SNPs)associated with NSAIDs indices were used as instrumental variables to estimate the associations with the risk of CVDs. The dataset was obtained from genome-wide association studies (GWAS). Estimation of the causal effect was mainly performed using the random effects inverse-variance weighted method (IVW). Furthermore, Cochran’s Q test, MR-Egger intercept tests, MR-PRESSO, leave-one-out analyses, and funnel plot were used in the sensitivity analysis.Results NSAIDs use increase causally the risks of coronary heart disease [CHD; odds ratio(OR) = 1.005, 95% confidence interval(CI): 1.002–1.007, P < 0.05], heart failure(HF; OR = 1.091; 95% CI, 1.027–1.160; P < 0.05), atrial fibrillation(AF; OR = 1.087; 95% CI, 1.034–1.142; P < 0.05) with multiplicative random-effects IVW MR analysis. However, there was no suggestive evidence of a causal association between NSAIDs use and myocardial infarction (MI; OR = 1.001; 95% CI, 0.999–1.003; P = 0.23), or hypertension(OR = 1.001; 95% CI, 0.995–1.007; P = 0.72). The main results are kept stable in the sensitivity analysis.Conclusion This MR study provided support for a causal association of NSAIDs use with CHD, HF, and AF. However, it did not support an association of genetically predicted NSAIDs use on MI, and hypertension.
Evaluation of Case Discussing Teaching by Multidisciplinary team combined with Case-Based Learning Approach in Gynecological Oncology
Authors: Zhou Bo , Zhao Mengya , Yuan Yunhe , Hu Zheng
Published: November 2023

Background: In gynecologic oncology, traditional teaching methods have been challenged due to the complex disease nature and the uniqueness of the patient. To address these challenges, innovative approaches are needed.
Methods: This study aims to assess the effectiveness of the multi-disciplinary team (MDT) combined with case-based learning (CBL) teaching methods in gynecological oncology education for standardized resident trainees. The study includes second and third-year resident trainees who underwent standardized training in our department from 2021 to 2022. These trainees were randomly assigned to either an experimental group or a control group, each consisting of 15 individuals. The experimental group received instruction using the MDT+CBL combined teaching model, while the control group followed the traditional CBL teaching model. We compared post-teaching theoretical assessment scores and clinical case analysis abilities between the two groups. Furthermore, we assessed trainee satisfaction with teaching effectiveness through a questionnaire survey.
Results: The experimental group achieved significantly higher scores in both theoretical knowledge assessment and clinical case assessment compared to the control group (t = 5.681 and 5.388, respectively, P < 0.05). Additionally, the experimental group expressed significantly greater satisfaction with the teaching effectiveness in terms of stimulating learning interest, mastering basic theoretical knowledge and skills, engaging in self-directed learning through literature review, enhancing clinical reasoning abilities, and improving teamwork cooperation abilities. All of these aspects displayed statistical significance (t = 5.046, 5.512, 4.838, 4.580, 9.707, respectively, P < 0.05).
Conclusions:
The MDT+CBL teaching model significantly enhances teamwork skills among students, improves the quality of gynecological oncology education, kindles students' enthusiasm for learning, promotes self-directed learning through literature review, enhances the application of relevant theoretical knowledge, and provides a more comprehensive understanding of diseases. Consequently, it substantially elevates overall teaching quality and enhances students' clinical thinking effectiveness. This approach is not only valuable for obstetrics and gynecology (OB/GYN) but also offers a promising model for medical education across disciplines.
Navigating the Complexities of Constructions: Unveiling the Dichotomy Between Added-Values and Applications of Risk Management.
Authors: Alrabie Mohammad
Published: November 2023

The different processes, developments, and advancements associated with the modern trend of constructions are complicated, rapid, and involve great risks that could impact projects. Thus, the necessity for an overall risk management approach in constructions is on a constantly rising trajectory. The paper discusses the added-values of implementing risk management within the construction industry, as well as differentiating between the added-values of risk management, and risk management applications within the projects. The paper also discusses and evaluates the reliability of the added-values of risk management presented in the literature review against the analysis results from the participants. The analysis results showed a significant positive correlation between the participants’ years of experience and their awareness of risk management’s applications. The analysis also demonstrated the variation in significance in risk management applications among participants, which concluded that there is a fundamental difference between the added-values and the applications of risk management.
Chronic kidney disease as a risk factor for severe preoperative anemia and heart failure in elderly patients with hip fracture: a single-center retrospective cohort study
Authors: Meng Yao , Wang Zhiqian , Hou Zhiyong
Published: November 2023

Background
Patients with chronic kidney disease (CKD) are prone to hip fractures and have a worse preoperative prognosis than patients without CKD. However, few studies have examined this issue by directly comparing patients with and without CKD.
Methods
We conducted a retrospective analysis of hip fracture patients ≥ 65 years of age who attended a level 1 trauma centre in China from January 2017 to December 2020. The CKD and non-CKD groups were matched 1:1 using the propensity score matching (PSM) method. Demographic data, comorbidities and preoperative complications were collected from the hospital's electronic medical records for both groups of patients. Patients with preoperative heart failure and anemia were classified into two classes based on different B-type natriuretic peptide (BNP) and haemoglobin (Hgb) levels. The above data were compared between the two groups of patients. The association between CKD and preoperative heart failure and anemia was assessed using univariate and multivariate logistic regression and subgroup analysis.
Results
We finally included 126 patients with CKD and 126 patients without CKD. The incidence of both preoperative heart failure (66.7% vs. 47.6%) and anemia (61.4% vs. 38.6%) was higher in CKD patients than in non-CKD patients. Mild heart failure (BNP < 500pg/ml) and severe heart failure (BNP ≥ 500pg/ml), mild anemia (Hgb ≥ 9.0g/dL) and severe anemia (Hgb < 9.0g/dL) were statistically different between the two groups (P < 0.05). By logistic regression analysis, patients with CKD were risk factors for severe heart failure (OR, 2.773; 95% CI, 1.299–5.922) and severe anemia (OR, 4.702; 95% CI, 1.982–11.157). After subgroup analyses, the above conclusions remain valid.
Conclusion
Patients with CKD have a higher risk of severe heart failure and anemia preoperatively than non-CKD patients. Clinicians should strengthen preoperative management of such patients and make full preparations for the prevention of serious complications.
Zwint is highly expressed in ovarian cancer and promotes cell proliferation, migration and invasion
Authors: Liu Wen-ting , Chen Ling , Fu Fen , Wan Xian-xian , Zhang Meng-meng
Published: November 2023

Objective: To investigate the expression level of ZWINT in ovarian cancer and its impact on the proliferation, migration and invasion of ovarian cancer cells.
Methods: The differentially expressed genes in ovarian cancer tissues were identified from both the TCGA and GEO databases, followed by an analysis of their expression levels and investigation into their impact on patients' survival and prognosis; two ovarian cell models were established to interfere with gene expression, and the expression and interference effects were evaluated by RT-PCR and Western blot; cell proliferation was detected by plate cloning and Edu assay.
Result: ZWINT was identified as a differentially expressed gene in ovarian cancer through database analysis, and further comparison of gene expression profiles revealed its high expression levels in this malignancy; Kaplan-Meier analysis revealed a correlation between higher expression of ZWINT and worse survival prognosis in patients. The success of interfering with the ZWINT cell line was verified through RT-PCR and Western blot experiments. Plate cloning assay demonstrated a significant reduction in the number of cell colonies after ZWINT interference, while Edu assay indicated a decrease in the percentage of Edu-positive cells. Transwell assay suggested that cell migration and invasion were also affected by ZWINT interference.
Conclusion: ZWINT was highly expressed in ovarian cancer tissues, and could promote the proliferation, migration and invasion of cancer cells.
Should hypervascular incidentalomas detected on per-interventional cone beam computed tomography during intra-arterial therapies for hepatocellular carcinoma impact the treatment plan in patients waiting for liver transplantation?
Authors: Derbel Haytham , Galletto Athena , Mulé Sébastien , Calderaro Julien , Zaarour Youssef , Saccenti Laetitia , Ghosn Mario , Reizine Edouard , Blain Maxime , Laurent Alexis , Brustia Raffaele , Leroy Vincent , Amaddeo Giuliana , Luciani Alain , Tacher Vania , Kobeiter Hicham
Published: November 2023

Background and objective Current guidelines do not indicate any comprehensive management of hepatic hypervascular incidentalomas (HVIs) discovered in hepatocellular carcinoma (HCC) patients during intra-arterial therapies (IAT). The objective of this study is to evaluate the prognostic value of HVIs detected on per-interventional cone beam computed tomography (CBCT) during IAT for HCC in patients waiting for liver transplantation (LT). Material and methods In this retrospective single-institutional study, all liver-transplanted HCC patients between January 2014 and December 2018 who received transarterial chemoembolization (TACE) or radioembolization (TARE) before LT were included. The number of ≥ 10 mm HCCs diagnosed on contrast-enhanced preinterventional imaging (PII) was compared to that detected on per-interventional CBCT with a nonparametric Wilcoxon test. The correlation between the presence of an HVI and histopathological criteria associated with poor prognosis (HPP) on liver explants was investigated using the chi-square test. Tumor recurrence (TR) and TR-related mortality were investigated using the chi-square test. Recurrence-free survival (RFS), TR-related survival (TRRS), and overall survival (OS) were assessed according to the presence of HVI using Kaplan‒Meier analysis. Results Among 63 included patients (average age: 59 ± 7 years, H/F = 50/13), 36 presented HVIs on per-interventional CBCT. The overall nodule detection rate of per-interventional CBCT was superior to that of PII (median at 3 [Q1:2, Q3:5] vs. 2 [Q1:1, Q3:3], respectively, p < 0.001). No significant correlation was shown between the presence of HVI and HPP (p = 0.34), TR (p = 0.095) and TR-related mortality (0.22). Kaplan‒Meier analysis did not show a significant impact of the presence of HVI on RFS (p = 0.07), TRRS (0.48), or OS (p = 0.14). Conclusion Detection of HVIs during IAT should not affect the IAT treatment plan in patients awaiting LT.
Light Weight Learning Algorithms for Profanity Detection
Authors: Khurdula Harsha Vardhan
Published: November 2023

Text classification, mainly in the realm of online interaction of people in general through text, plays a vital role in maintaining safety over any social platform. Especially, in this age where it is easy to go online and type without filter. This research focuses on a comparative study of two simple yet prominent classifiers - Logistic Regression (LR) and Naive Bayes - to classify provided text as either toxic or non toxic. This research identifies potential improvements through iterative training and data processing enhancements.
ChatGPT in Oral and Maxillofacial Radiology Education
Authors: Öztürk Hilal Peker , Avsever İsmail Hakan , Şenel Buğra , Ayran Şükran , Özgedik Hatice Seda , Baysal Nurten
Published: November 2023

Introduction
Artificial intelligence refers to the ability of computer systems or machines to perform cognitive functions and tasks that are similar to those of humans.
The aim of this study is to assess the knowledge and interpretative abilities of ChatGPT-versions by administering a dentomaxillofacial radiology exam, comparing its performance with that of dentistry-students in Türkiye, and questioning the effectiveness of different language options.
Methods
This study is a descriptive research comparing the data obtained by presenting the midterm exam questions, which were routinely administered to 4thyear dental students, to ChatGPT versions 3.5 and 4 in both Turkish and English.
Results
Firstly the 20 test questions were evaluated. The p-value was <0.05, there is a significant difference between the ChatGPT answer sheets. ChatGPT-4 in English demonstrated the highest performance.
Answer sheets of chatGPT-4 in Turkish and in English demonstrated the best performance with 5 correct answers in open-ended questions.
Based on the answers obtained from 89 students to 20 test questions, a class profile was created. ChatGPT answer sheets and class-profile were analyzed, the p-value was p<0.05. Class-profile ranked first as ChatGPT-4 in English.
It was found a significant difference between the answer-sheets of ChatGPT and the class-profile for open-ended questions(p<0.10). The most successful results were obtained from ChatGPT-4 in Turkish and English, as well as the class profile.
Conclusion
As a cautionary note to dental students, it is important to mention that ChatGPT's knowledge and perception in the field of oral and maxillofacial radiology are not yet sufficient, particularly for use in examinations.
MODELS: a novel six-step framework for developing an infectious disease model
Authors: Rui Jia , Li Kangguo , Wei Hongjie , Guo Xiaohao , Zhao Zeyu , Wang Yao , Song Wentao , Abudunaibi Buasiyamu , Chen Tianmu
Published: November 2023

During the COVID-19 pandemic, numerous modeling studies about COVID-19 have been published. While partial articles exhibit clever design and are noticeable, some also contain methodological errors. For beginners and primary health workers, grappling with the diverse content of these articles is challenging. Therefore, we develop a model framework (MODELS) that outlines the process and considerations for establishing a reliable epidemic model. Our work provides beginner guidance to researchers who are interested in epidemic models.
Edges are all you need: Potential of Medical Time Series Analysis with Graph Neural Networks.
Authors: Walke Daniel , Steinbach Daniel , Gibb Sebastian , Kaiser Thorsten , Saake Gunter , Ahrens Paul , Broneske David , Heyer Robert
Published: November 2023

Machine learning is a powerful tool to develop algorithms for clinical diagnosis. However, standard machine learning algorithms are not perfectly suited for clinical data since the data are interconnected and may contain time series. As shown for recommender systems and molecular property predictions graph neural networks (GNNs) may represent a powerful alternative. In this study, we evaluated the performance and time consumption of GNNs compared to state-of-the-art algorithms on the classification of sepsis from blood count data as well as the importance and slope of each feature for the final classification. GNNs on evaluated similarity-graphs achieved an AUROC of up to 0.8747 comparable to the performance of ensemble-based machine learning algorithms and a neural network. The exploitation of time series information with GNNs achieved a superior AUROC of up to 0.9565. Finally, we discovered that feature slope and importance highly differ between tree-based (e.g., XGBoost) and non-tree-based algorithms (e.g., GNN).
Nanoemulsion-based active packaging for food products
Authors: Prasad Jaishankar , Dixit Aishwarya , Sharma Sujata P. , Mwakosya Anjelina W. , Petkoska Anka T. , Upadhyay Ashutosh , Kumar Nishant
Published: November 2023

Recently, there has been an increasing trend in the food and pharmaceutical industries towards using nanotechnological approaches to drug delivery and active packaging (edible coatings and films). In the food sector, nanoemulsions are the most promising technology for delivering active components and improving the barrier, mechanical, and biological properties of packaging to ensure the safety and quality of food products, as well as extend their shelf life.

For this review, we used several databases (Google Scholar, Science Direct, PubMed, Web of Science, Scopus, Research Gate, etc.) to collect information about nanoemulsions and their role in edible packaging.

We searched for articles published between 2015 and 2022 and described different scientific approaches to developing active packaging systems based on nanoemulsions, as well as their high-energy and low-energy synthesis methods. We also reviewed the uses of different types of essential oil-based nanoemulsions in the packaging of food products to prolong their shelf life and ensure safety. Non-migratory active packaging and active-release packaging systems were also discussed, as well as their advantages and disadvantages.
Formação de professores e políticas públicas educacionais na Ibero-América
Authors: Rodriguez Milagros Elena , Fortunato Ivan , Araújo Osmar Hélio
Published: November 2023

Neste artigo, por meio da Análise Crítica do Discurso, discute-se a relação entre Políticas Públicas e Formação Docente na região Ibero-Americana, a partir das vozes de docentes entrevistados. Questionamos: como estes conceitos (decolonialidade, complexidade, entre outros) são vistos nas políticas públicas educacionais de seu país?, como são convertidos em ações efetivas nas instituições formadoras?, como se articulam as políticas públicas e como são efetivamente desenvolvidas nas universidades?. Os resultados não são animadores e em muitos países as contribuições das políticas educacionais estatais em prol da formação de professores são graves. Há um pedido de socorro em meio às vozes dos entrevistados, por isso continuamos a insistir e persistir rumo a uma educação libertadora.
LIMD2 is the Signature of Cell Aging-immune/Inflammation in Acute
Myocardial Infarction
Authors: Tao Ping , Chen Xiaoming , Xu Lei , Chen Junteng , Nie Qinqi , Xu Mujuan , Feng Jianyi
Published: November 2023

Background:

Acute myocardial infarction (AMI) is an age-dependent cardiovascular
disease in which cell aging, immunity, and inflammatory factors alter the course; however, cell
aging-immune/inflammation signatures in AMI have not been investigated.

Methods:

Based on the GEO database to obtain microRNA (miRNA) sequencing, mRNA sequencing
and single-cell sequencing data, and utilizing the Seurat package to identify AMI-associated
cellular subpopulations. Subsequently, differentially expressed miRNAs and mRNAs
were screened to establish a network of competing endogenous RNAs (ceRNAs). Senescence
and immunity scores were calculated by single sample gene set enrichment analysis (ssGSEA),
ESTIMATE and CIBERSORT algorithms, and the Hmisc package was used to screen for genes
with the highest correlation with senescence and immunity scores. Finally, protein-protein interaction
(PPI) and molecular docking analyses were performed to predict potential therapeutic
agents for the treatment of AMI.

Results:

Four cell types (Macrophage, Fibroblast, Endothelial cells, CD8 T cells) were identified
in AMI, and CD8 T cells exhibited the lowest cell aging activity. A ceRNA network of miRNAs-
mNRA interactions was established based on the overlapping genes in differentially expressed
miRNAs (DEmiRNAs) target genes and differentially expressed mRNAs (DEmRNAs).
Twenty-four marker genes of CD8 T cells were observed. LIMD2 was identified as cell aging-
immune/inflammation-related hub gene in AMI. This study also identified a potential therapeutic
network of DB03276-LIMD2-AMI, which showed excellent and stable binding status between
DB03276-LIMD2.

Conclusion:

This study identified LIMD2 as a cell aging-immune/inflammation-related hub
gene. The understanding of the pathogenesis and therapeutic mechanisms of AMI was enriched
by the ceRNA network and DB03276-LIMD2-LAMI therapeutic network.