bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Case of Pregnancy and Follow-Up of Bartter Syndrome (Type II) with a Novel Mutation
Authors: Lūse Laura , Miskova Anna , Rezeberga Dace , Jansone Gita , Rasnača Kristīne , Lūse Laura , Vedmedovska Natālija , Kovale Sabīne
Published: May 2020

Background: Bartter syndrome is a rare autosomal recessive inherited salt wasting tubulopathy, it`s
incidence proportion is 1.2 cases per 1.000.000 live births. The present case - report discusses a clinical case
of an antenatal Bartter syndrome (type II) with a novel mutation and it`s course from antenatal presentation
to 6 months postpartum.
Case Presentation: The case-report discusses a clinical case of an antenatal Bartter syndrome (type II) with
a novel homozygous missense variant mutation in KCNJ1 gene: c.554C>T (p. Pro185Leu). Symptoms
presented from 24 weeks of pregnancy as premature labour threats, maternal dyspnoea and severe
polyhydramnios (amniotic fluid index 36 cm). Therapeutic interventions included use of indomethacin,
dexamethasone, micronized progesterone and three consequent amnioreductions. Pregnancy was prolonged
until 32 weeks and induced due to severe reoccurring polyhydramnios, progressing maternal dyspnoea and
inability to perform next amnioreduction. Labour was complicated by severe placental abruption and new
born – boy was referred to neonatal intensive care unit. Neonatal period was complicated by electrolyte
abnormalities: hyponatremia, hypochloremic metabolic alkalosis, transient hyperkalaemia that gradually
developed into hypokalaemia, hypercalcemia and elevated rennin and aldosterone levels characteristic to
type II Bartter syndrome. At 6 months (corrected age 4 months) he is gaining weight within normal ranges
and his psychomotor development is ahead of his corrected age, without any need for daily medications.
Conclusion: The present case report describes the clinical course of a Bartter syndrome is of high
importance, due to the reason that it shows clinical course of patient with novel mutation and offers one of
the ways how to manage the disease. The described novel mutation may have favourable prognosis for
neonate. The pregnancy should be managed as high-risk pregnancy with expertise in perinatal diagnostics
and interventions. Early recognition, and interventions, are and essential to prolong a pregnancy and lessen
prematurity complications.
Gastro-Splenic Fistula from Diffuse Large B-Cell Lymphoma: Case Report
Authors: Smith Brian R. , Smith Brian R. , Smith Ellyn , N Smith Lauren
Published: May 2020

A gastro-splenic fistula is a rare complication of lymphoma, the etiology of which is poorly understood due
to the narrow spectrum of cases. Additional case reports of this complication allow for increased
understanding to facilitate treatment planning. This case report provides our detailed patient scenario and
surgical approach to a gastro-splenic fistula in the setting of large B-cell lymphoma and compares this
approach to similar cases provided in the literature review
Comparative efficacy of autologous versus cadaveric saphenous vein grafts in cerebral revascularization surgery
Authors: Stapleton Christopher J. , Hussein Ahmed E. , Behbahani Mandana , Alaraj Ali , Amin-Hanjani Sepideh , Charbel Fady T.
Published: May 2020

OBJECTIVE

Cerebral bypasses are performed for the purpose of either flow augmentation for ischemic cerebrovascular disease or flow replacement for vessel sacrifice during complex aneurysm or tumor surgery. Saphenous vein grafts (SVGs) are commonly used interposition grafts. The authors of this study sought to compare the efficacy of autologous versus cadaveric SVGs in a large series of cerebral bypasses using interposition vein grafts with long-term angiographic follow-up.

METHODS

All intracranial bypass procedures performed between 2001 and 2018 were reviewed. Demographic, clinical, angiographic, and operative data were recorded and then analyzed according to SVG type.

RESULTS

A total of 308 consecutive intracranial bypasses were performed during the study period, 53 (17.2%) of which were bypasses with an interposition SVG (38 autologous, 15 cadaveric). At a median follow-up of 2.2 months (IQR 0.2–29.1), 39 (73.6%) bypasses were patent (26 [68.4%] autologous, 13 [86.7%] cadaveric, p = 0.30). Comparing autologous and cadaveric SVG recipients, there were no statistically significant differences in age (p = 0.50), sex (p > 0.99), history of smoking (p = 0.75), hypertension (p > 0.99), diabetes mellitus (p = 0.13), indication for bypass (p = 0.27), or SVG diameter (p = 0.65). While there were higher intraoperative (autologous, 100.0 ml/min, IQR 84.3–147.5; cadaveric, 80.0 ml/min, IQR 47.3–107.8; p = 0.11) and postoperative (autologous, 142.2 ml/min, IQR 76.8–160.8; cadaveric, 92.0 ml/min, IQR 69.2–132.2; p = 0.42) volumetric flow rates in the autologous SVGs compared to those in the cadaveric SVGs, the difference between the two groups did not reach statistical significance. In addition, the blood flow index, or ratio of postoperative to intraoperative blood flow, for each bypass was similar between the groups (autologous, 1.3, IQR 0.9–1.6; cadaveric, 1.5, IQR 1.0–2.3; p = 0.37). Kaplan-Meier estimates showed no difference in bypass patency rates over time between autologous and cadaveric SVGs (p = 0.58).

CONCLUSIONS

Cadaveric SVGs are a reasonable interposition graft option in cerebral revascularization surgery when autologous grafts are not available.
Critical role of platelet-derived growth factor–α in angiogenesis after indirect bypass in a murine moyamoya disease model
Authors: Hayashi Tomohide , Yamamoto Seiji , Hamashima Takeru , Mori Hisashi , Sasahara Masakiyo , Kuroda Satoshi
Published: May 2020

OBJECTIVE

This study aimed to clarify the underlying mechanism of pathognomonic angiogenesis between the temporal muscle and neocortex after indirect bypass for moyamoya disease by shedding light on the role of platelet-derived growth factor receptor–α (PDGFRα) in angiogenesis.

METHODS

The gene for PDGFRα was systemically inactivated in adult mice (α-KO mice). The Pdgfra-preserving mice (Flox mice) and α-KO mice were exposed to bilateral common carotid artery stenosis (BCAS) by using microcoils. One week later the animals underwent encephalomyosynangiosis (EMS) on the right side. Cerebral blood flow (CBF) was serially measured using a laser Doppler flowmeter. Histological analysis was performed on the distribution of CD31-positive vessels and collagen deposit at 28 days after BCAS. Reverse transcription polymerase chain reaction (RT-PCR) was performed to assess the expression of collagen mRNA in the skin fibroblasts derived from Flox and α-KO mice.

RESULTS

BCAS significantly reduced CBF up to approximately 70% of the control level at 28 days after the onset. There was no significant difference in CBF between Flox and α-KO mice. EMS significantly enhanced the improvement of CBF on the ipsilateral side of Flox mice, but not α-KO mice. EMS significantly induced the development of CD31-positive vessels in both the neocortex and temporal muscle on the ipsilateral side of Flox mice, but not α-KO mice. Deposition of collagen was distinctly observed between them in Flox mice, but not α-KO mice. Expression of mRNA of collagen type 1 alpha 1 (Col1a1) and collagen type 3 alpha 1 (Col3a1) was significantly downregulated in the skin fibroblasts from α-KO mice.

CONCLUSIONS

This is the first study that denotes the role of a specific growth factor in angiogenesis after EMS for moyamoya disease by inactivating its gene in mice. The findings strongly suggest that PDGFRα signal may play an important role in developing spontaneous angiogenesis between the temporal muscle and neocortex after EMS in moyamoya disease.
Compassionate use of a custom 3D-printed sacral implant for revision of failing sacrectomy: case report
Authors: Chatain Grégoire P. , Finn Michael
Published: May 2020

Reconstruction of the spinopelvic continuity after sacral resection for primary sacral tumors remains challenging. Complex anatomical and biomechanical factors of this transition zone may be addressed with the advancement of 3D-printed implants. Here, the authors report on a 67-year-old patient with a sacral chordoma who initially underwent total en bloc sacrectomy followed by standard spinopelvic reconstruction. Pseudarthrosis and instrumentation failure of the lumbosacral junction construct subsequently developed. A custom 3D-printed sacral prosthesis was created using high-resolution CT images. Emergency Food and Drug Administration approval was obtained, and the custom device was implanted as a salvage reconstruction surgery. Made of porous titanium mesh, the custom artificial sacrum was placed in the defect based on the anticipated osteotomic planes and was fixed with a screw-rod system along with a fibular bone strut graft. At the 18-month follow-up, the patient was disease free and walking short distances with assistance. CT revealed excellent bony incorporation into the graft.

The use of a custom 3D-printed prosthesis in spinal reconstruction has been rarely reported, and its application in sacral reconstruction and long-term outcome are novel. While the implant was believed to be critical in endowing the region with enough biomechanical stability to promote healing, the procedure was difficult and several key learning points were discovered along the way.
Penerapan Lesson Study dalam Perkuliahan Matematika Dasar pada Mahasiswa Pendidikan Biologi Universitas Pasir Pengaraian
Authors: Isharyadi Ratri , Eka Afri Lusi , Annajmi Annajmi
Published: May 2020

Penelitian ini bertujuan untuk mendeskripsikan pembelajaran matematika dasar melalui kegiatan lesson study bagi mahasiswa pendidikan biologi universitas pasir pengaraian.  Penelitian ini dilaksanakan di program studi Pendidikan Biologi Universitas Pasir Pengaraian dengan subjek penelitian adalah mahasiswa S-1 yang mengambil mata kuliah matematika dasar tahun akademik 2019/2020 semester ganjil. Pelaksanaan penelitian menggunakan tahapan plan, do dan see. Instrument penelitian berupa lembar observasi, rekaman video dan hasil pekerjaan mahasiswa berupa lembar kerja mahasiswa. Data hasil observasi dianalisis secara deskriptif, data hasil belajar matematis peserta didik dianalisis untuk mengetahui proses pembelajaran matematika dasar melalui lesson study.  Pelaksanaan Lesson Study di perkuliahan Matematika Dasar secara garis besar berjalan dengan baik. Pelaksanaan pembelajaran berjalan sesuai dengan yang direncanakan. Selama proses pembelajaran, mahasiswa terlibat aktif dalam mempelajari materi perkuliahan. Hasil belajar yang diperoleh dari turnamen diakhir pelajaran menunjukkan tercapainya tujuan pembelajaran. Pelaksanaan lesson study dapat memberikan wadah untuk para pendidikan saling berbagi pengalaman tentang praktek terbaik dalam pembelajaran. Kolaborasi antar dosen dalam mempersiapkan dan melaksanakan pembelajaran memberikan hasil yang baik dalam proses dan hasil belajar mahasiswa. Rangkaian kegiatan pembelajaran tersebut ternyata dapat meningkatkan kualitas proses pembelajaran dan juga pemahaman mahasiswa dalam memahami materi perkuliahan. Walaupun demikian, masih terdapat beberapa perbaikan yang harus dilakukan untuk kedepannya
Evaluation of intercellular communication between normal human prostate epithelial cells and prostate cancer cell lines in co-culture model
Authors: Samli Hale , Samli DuruTalya , Ardicli Sena , Samli Murat , Vatansever Buse
Published: May 2020

BACKGROUND:

Recent evidences have provided new aspects to metastasis formation with respect to intercellular communication/interaction.

OBJECTIVE:

The purpose of this research was to present an in vitro co-culture-principle application for intercellular communication between normal human prostate epithelial cells and prostate cancer cell lines.

METHODS:

Two prostate cancer cell-lines including androgen-independent DU-145 and PC-3 and the PNT1A, normal human prostate epithelial cell-line, were used in co-culture design. Prostate cancer cells and normal prostate epithelial cells were allowed to grow on the same culture medium without direct cell-to-cell contact and to communicate with each other. After a period of six-days, expression profiles of seven marker genes, including EGFR, SOX2, CD133, CD44, CASPASE 3, CASPASE 9, and IL-6 were determined using qPCR analysis. Comparison of experimental co-cultures with control groups was performed based on repetitive measurements.

RESULTS:

Expression analysis indicated that several of the genes were expressed at different levels in DU145 and PC-3 cells co-cultured with PNTA1. In this context, SOX2, CASPASE 9, and IL-6 genes were over-expressed up to 2–16 times in co-culture set up.

CONCLUSIONS:

This study may provide important clues regarding intercellular communication between normal and cancer cell lines. However, further experiments are needed to confirm the present results and to evaluate novel aspects of cancer development.
Contents Vol. 33 no. 2 July – December 2018
Authors: Head and Neck Surg Philipp J Otolaryngol
Published: May 2020

EDITORIAL

4 A Dozen Years, A Dozen Roses

Lapeña JF

ORIGINAL ARTICLES

6 Efficacy of Clarithromycin Versus Methylprednisolone in the Treatment of Non-Eosinophilic and Eosinophilic Nasal Polyposis: A Randomized Controlled Trial

Gammad JC, Chua AH, Templonuevo-Flores CS

14 Simulation Platform for Myringotomy with Ventilation Tube Insertion in Adult Ears

Chan AL, Carrillo RJD, Ong KC

21 Prevalence of Supraorbital Ethmoid Air Cells among Filipinos

Carlos ALC, Gelera JE

24 Recurrent Laryngeal Nerve Paralysis and Hypocalcemia in Superior to Inferior Compared to Inferior to Superior Dissection Approaches in Thyroidectomy

Yu RD

28 Pathologic Laryngoscopic Findings, Number of Years in Teaching, and Related Factors among Secondary Public-School Teachers in Bacolod City, Negros Occidental

Mundo NP, Vinco VV

32 Tracheal Diameter Estimates Using Age-Related Formula Versus Radiographic Findings: Which Approximates the Actual Tracheostomy Tube in Pediatric Patients?

De Guzman ICS

CASE REPORTS

37 Lethal Midline Granuloma in a 15-Year-Old Girl: A Diagnostic Dilemma

Gonzales ICA

41 Tuberculosis of the Temporomandibular Region

Santos JM, Reala ET

45 Crab Shell Impaction in the Larynx with Aphonia

Parekh NM, Kashyap PR

SURGICAL INNOVATION AND INSTRUMENTATION

48 PHONETOVOX: A Novel Device for Alaryngeal Speech

Econ ME, Soriano RG

53 Can Modified Laryngosternopexy (Laryngoclaviculopexy) Project the Larynx Anteriorly?

Carrillo RJC, Lapeña JF

FEATURED GRAND ROUNDS

56 Cleft Beyond the Lip and Palate: A Bilateral Tessier Cleft

Canta LAB

FROM THE VIEWBOX

60 Pulsatile Tinnitus Due to a Sigmoid Sinus Diverticulum and/or Dehiscence

Yang NW

UNDER THE MICROSCOPE

62 Respiratory Epithelial Adenomatoid Hamartoma

Carnate JM, Abelardo AD

PASSAGES

64 Armando T. Chiong, Sr. (1930 - 2018)

Jamir JC

65 Carlos P. Dumlao (1950 - 2018)

Pontejos AQY
One in Research as One PSO-HNS
Authors: Poblete Danilo
Published: May 2020

Research is the tool that unravels what people have seen and ponder on ideas which nobody else has thought. As Neil Armstrong stated, Research is creating new knowledge. This percipience ignites development of every individual.

For this reason, the Philippine Society of Otolaryngology - Head and Neck Surgery (PSO-HNS) is committed to its objective of contributing insights and skills in the diagnosis and treatment of ENT-Head and Neck ailments. Fellows Research Grants are available to augment the expenses incurred during the process.

The various research contests hosted by the society through the commendable efforts and passion of our Vice-President and concurrent Scientific Chair Dr. Erasmo Gonzalo D. V. Llanes will continue to be the source of academic writings for the internationally recognized Philippine Journal of Otolaryngology Head and Neck Surgery (PJOHNS).

Our society recognizes the wisdom and leadership of the PJOHNS Editor-in-Chief Dr. Jose Florencio F. Lapeña, Jr. in maintaining the international stature of our publication

However, the task should be a collaborative effort of everyone and not limited to our training institutions.

We should be One in Research as One PSO-HNS! We should be One in Research as One PSO-HNS!
Contents Vol. 34 no. 1 January – June 2019
Authors: Head and Neck Surg Philipp J Otolaryngol
Published: May 2020

EDITORIAL

4 Authorship Controversies: Gift, Guest and Ghost Authorship

Lapeña JF

META-ANALYSIS

6 Morbidity Outcomes of Prophylactic Central Neck Dissection with Total Thyroidectomy versus Total Thyroidectomy Alone in Patients with Node-Negative Papillary Thyroid Cancer: A Meta-Analysis of Observational Studies

Sison CZI, Fernando AF, Gutierrez TMDG

ORIGINAL ARTICLES

14 Levothyroxine versus Levothyroxine with Iodine in Reduction of Thyroid Nodule Volume: A Double-Blind Randomized Controlled Trial

Segocio DJD, Cachuela JE

20 The Use of Bony Septum as an Extended Spreader Graft in Primary and Secondary Rhinoplasty

Que-Ansorge C, Yap EC

26 Clinical Profile of Filipino Patients with Epistaxis in a University Hospital

Gutierrez TMD, Lerma FJV

30 Advanced Laryngotracheal Stenosis Patients in a Tertiary Provincial Government Hospital: A Prospective Case Series

Villanueva JVM, Soriano RG

34 Maxillary Sinus Squamous Cell Carcinoma in a Tertiary Hospital in the Philippines

Hernandez AKM, Cabungcal ACA

38 Usability of a Smartphone Application for Preoperative Facial Analysis for Rhinoplasty among ENT Surgeons

Padua PFC, Dela Cruz APIC, Pascual RC, Cambe SMM

CASE REPORTS

44 Aggressive Tuberculous Otitis Media in a Young Child

Canta LAB, Dizon APJE, Abes FLLB

48 Non-Traumatic Cerebrospinal Fluid Leak from a Sphenoid Sinus Midline Roof Defect Previously Managed as Allergic Rhinitis

Formalejo JPD, Amable JPM

52 Arteriovenous Malformation of the Mandible in a Young Postpartum Woman

Mercado GAG, Delovino KAE, Carpela AB

56 Intraosseus Arteriovenous Malformation of the Mandible: Extracorporeal Curettage and Immediate Replantation

Tongol EA, Pontejos AQY, Fullante PB, Cabungcal ACA, Ong KMC

SURGICAL INNOVATION AND INSTRUMENTATION

60 Autologous Tracheal Cartilage Composite Graft for a Subglottic Defect after Laryngotracheal Resection for Invasive Papillary Thyroid Carcinoma

Crisostomo MV, Ureta CV

FEATURED GRAND ROUNDS

64 Solitary Fibrous Tumor of the Larynx and Anterior Neck

Pabayos GS, Chiong AM

UNDER THE MICROSCOPE

68 Intracapsular Carcinoma ex Pleomorphic Adenoma

Carnate JM, Masalunga MC
Contents Vol. 34 no. 2 July – December 2019
Authors: Head and Neck Surg Philipp J Otolaryngol
Published: May 2020

EDITORIAL

4 Open Access: DOAJ and Plan S, Digitization and Disruption Lapeña JF

ORIGINAL ARTICLES

7 Nasopharyngeal Tuberculosis in a Philippine Tertiary General Hospital

Gomez MAT, Villarta RL, Caro RM, Manasan CVC, Carnate JM

11 Salivary pH and Taste Sensitivity among Geriatric and Non-Geriatric Patients in a Tertiary Hospital: A Cross-Sectional Study

Crisostomo MV, Ureta CV

16 Timing of Tracheostomy, Weaning from Mechanical Ventilation and Duration of Hospitalization among a Sample of Pediatric Patients

Ferrolino JBA, Lapeña JFF, Carrillo RJD

20 Timing of Tracheostomy and Outcomes in Adults with Moderate and Severe Tetanus: A Cross-Sectional Study

Espinosa WZ, Vinco VV

24 Association of Ultrasonographic Findings and Thyroid Malignancy: A Cross-Sectional Study

Trinchera CS, Cruz ETS

29 Maxillofacial Gunshot and Blast Injuries Seen in a Tertiary Military Hospital

Garimbao JFP

32 Facial Paralysis in Longitudinal versus Oblique and Otic-Sparing versus Non Otic-Sparing Temporal Bone Fractures

Chua RJ, Lacanilao RC

CASE REPORTS

35 Kikuchi-Fujimoto Disease in a Filipino Boy: A Case Report

Escalderon JRDJ, Monroy AA

39 Congenital Muscular Torticollis: A Case Report

Villanueva LMB

42 Emergent Reconstruction of Laryngeal Penetrating Neck Injury: A Case Report

Pecolera RS, Onofre RDC

47 Autologous Fat Transfer to Improve Aesthetic Appearance in Facial Asymmetry from Parry-Romberg Syndrome: A Case Report

Sison MNK, Cruz ETS, Fernandez MAR

FEATURED GRAND ROUNDS

52 Left Hemifacial Lymphatic Malformation in a Nine-Year-Old Boy

Cambe SMM, Rabo JIS, Navarro-Locsin CGS

UNDER THE MICROSCOPE

55 Traumatic Ulcerative Granuloma with Stromal Eosinophilia

Inoferio KSA, Agbay RLM, Carnate JM
VIII Reunión del Grupo Fomento Social
Authors: Camacho Laraña, S.I. Ildefonso
Published: May 2020

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Comunicado final IV Asamblea Andalucía Acoge
Authors: Yare Cobos Santiago
Published: May 2020

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"Las ONGD y la crisis del desarrollo. Un análisis de la cooperación en Centroamerica" (Recensión)
Authors: Morales Gutiérrez Alfonso Carlos
Published: May 2020

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Elecciones municipales en un contexto de crisis e incertidumbre
Authors: Redacción Consejo de
Published: May 2020

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II Reunión del grupo de ética de las profesiones de la COCESU
Authors: Camacho Laraña, S.I. Ildefonso
Published: May 2020

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"La primacía de la sociedad civil. El proceso de formación de la España democrática" (Recensión)
Authors: Camacho Laraña, S.I. Ildefonso
Published: May 2020

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Reseñas
Authors: Rul-lán Buades Gaspar , Romero Rodríguez José Juan , Camacho Laraña Ildefonso , Prieto Martínez Fernando , Morales Gutiérrez Alfonso Carlos
Published: May 2020

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PENERAPAN MODEL PEMBELAJARAN REACT UNTUK MENINGKATKAN SIKAP ILMIAH DAN HASIL BELAJAR PESERTA DIDIK KELAS IX B SMP N 3 SELOMERTO TAHUN PELAJARAN 2018/2019
Authors: Wati Yudi Setya
Published: May 2020

Tujuan penelitian ini untuk meningkatkan sikap ilmiah dan hasil belajar menggunakan model pembelajaran REACT .Penelitian dilaksanakan pada bulan Juli sampai dengan Desember 2018, semester gasal Tahun Pelajaran 2018/2019. Yang menjadi subyek penelitian adalah Peserta Didik kelas IXB SMP Negeri 3 Selomerto. Metode yang digunakan dalam penelitian ini adalah Penelitian Tindakan Kelas.Teknik pengumpulan data dilakukan secara kualitatif dan  kuantitatif. Untuk mengungkap data sikap ilmiah digunakan lembar observasi, sedangkan hasil belajar diukur menggunakan soal pada tiap-tiap akhir siklus. Data kuantitatif untuk mengukur peningkatan hasil belajar, dianalisis dengan analisis deskriptif komparatif yaitu membandingkan nilai pra siklus, siklus 1 dan siklus 2. Data kualitatif untuk mengukur sikap ilmiah dianalisis menggunakan analisis deskriptif kualitatif.Dari hasil penelitian pengolahan dan analisis data ,  terjadi peningkatan sikap ilmiah dan hasil belajar peserta didik . Dari pra siklus sampai  siklus 2  terjadi peningkatan sikap ilmiah cukup signifikan yakni rata-rata 60,72 menjadi 75,00, sedangkan rata-rata hasil belajar meningkat dari 68,18 menjadi 79,5.  Hasil penelitian menunjukkan bahwa penerapan model REACT dapat meningkatkan sikap ilmiah dan hasil belajar IPA.
PENGGUNAAN VIDEO REFLEKTIF UNTUK MENINGKATKAN KEMAMPUAN MENGOMUNIKASIKAN HASIL PENGAMATAN BAGI PESERTA DIDIK KELAS VIIG SMPN 1 WONOSOBO PADA SEMESTER II TAHUN PELAJARAN 2017/2018
Authors: Priyanto Heri
Published: May 2020

Tujuan penelitian  ini adalah untuk meningkatkan kemampuan mengomunikasikan hasil pengamatan dan hasil belajar bagi peserta didik kelas VIIG SMPN 1 Wonosobo pada semester II tahun pelajaran 2017/2018 dengan menggunakan video reflektif. Penelitian tindakan kelas ini dilaksanakan selama dua siklus. Subyek penelitian adalah peserta didik kelas VIIG SMPN 1 Wonosobo pada tahun pelajaran 2017/2018. Data dikumpulkan dengan menggunakan lembar pengamatan dan tes hasil belajar peserta didik. Selanjutnya data dianalisis dengan menggunakan metode deskriptif kualitatif. Hasil penelitian ini menunjukkan bahwa kemampuan mengomunikasikan peserta didik meningkat dari 65  menjadi 78 pada akhir siklus I dan 94 pada akhir siklus I dan II. Hasil penelitian juga menunjukkan bahwa penggunaan video reflektif dapat meningkatkan nilai rata-rata hasil ulangan harian dari 80 menjadi 93 pada akhir siklus II. Pada aspek sikap peserta didik terdapat perubahan pada peduli lingkungan, kerja sama, dan mandiri dari 34, 63, dan 29 menjadi 94, 99, dan 89 pada akhir siklus II.
Fabrication of Nanofiltration Membrane Utilising Surfactant Via Non-Solvent Induced Phase Separation Method
Authors: Mohamat Rosmanisah
Published: May 2020

In this work, graphene oxide (GO) and titanium dioxide (TiO2) were used as an additive to fabricate the nanofiltration (NF) membrane. GO was synthesised via electrochemical exfoliation method utilising sodium bis(3,5,5-trimethyl-1-hexyl) sulphosuccinate (AOT4) surfactant. The synthesised GO was then used to fabricate PVDF-based NF membrane namely PVDF/GO_TiO2 via non-solvent induced phase separation (NIPS) method. The effects of embedded GO and TiO2 on the morphology and structural properties of PVDF/GO_TiO2 were investigated by using field emission scanning electron microscopy (FESEM) and micro-Raman spectroscopy. The finding shows that PVDF/GO_TiO2 present thin and dense top layer supported by macro-voids sub-layer with sponge-like layer at the bottom. Based on its morphology, the fabricated PVDF/GO_TiO2 membrane is potential to be applied as membrane filtration for water treatment application.
ANALISIS BENCANA TANAH LONGSOR SERTA MITIGASINYA
Authors: Fatiatun Fatiatun , Firdaus Firdaus , Jumini Sri , Adi Nugroho Prasetya
Published: May 2020

Analisis kejadian tanah longsor ini dilatarbelakangi oleh banyaknya bencana yang terjadi di Wonosobo. Tanah longsor merupakan salah satu bencana yang sering terjadi di daerah pegunungan, khususnya desa Tieng yang ada di Wonosobo. Masyarakat di daerah tersebut diantisipasi untuk selalu waspada terhadap bencana tanah longsor yang selalu mengancam di saat datangnya musim hujan. Cara penanganan yang tepat saat dan setelah terjadinya bencana tanah longsor merupakan hal yang sangat penting dimiliki oleh warga masyarakat sekitar daerah kejadian tersebut. Teknik survei dipakai dalam menganalisis bencana tanah longsor yang ada di daerah Wonosobo ini. Banyak korban jiwa yang meninggal, luka-luka dan hilang akibat bencana tanah longsor ini. Mitigasi bencana tanah longsor yang dilakukan pemerintah dan warga masyarakat sekitar bencana yang juga dibantu oleh para pecinta alam adalah dengan melakukan reboisasi dengan tanaman yang bisa menyerap banyak air dan menahan tanah supaya tidak longsor lagi. Kegiatan reboisasi ini disampaikan oleh pemerintah ke warga masyarakat sekitar kejadian tanah longsor melalui kegiatan penyuluhan tanggap bencana.
Agua no potable para consumo en la Horticultura de La Plata (Buenos Aires). Causas, consecuencias y alternativas
Authors: Garcia Matias , Rozadilla Gastón , Cano Leonardo
Published: May 2020

En la región periurbana de La Plata se destaca la producción de hortalizas frescas llevada a cabo por pequeños agricultores familiares. En el marco de las problemáticas de hábitat y salud, se observa una serie de sintomatologías que afectan tanto a los productores/trabajadores y sus familias -principalmente los chicos que allí habitan- como así también a los consumidores de los productos hortícolas. Estas sintomatologías tienen como origen principal la utilización de agua no potable. El trabajo se propone determinar el tipo y magnitud de la contaminación del agua, identificar los causales del mismo y evaluar alternativas que resuelvan el presente inconveniente. Los resultados indican que una problemática compleja como la planteada, requiere que las soluciones tecnológicas sean acompañadas de acciones políticas que modifiquen condiciones estructurales de la vida de los productores en el cinturón hortícola platense.
La escritura de docentes practicantes: sobre la enseñanza de Lengua y Literatura
Authors: Tapia Stella Maris
Published: May 2020

Se describen prácticas de escritura desarrolladas por docentes practicantes durante el dictado de la asignatura Planificación y Práctica Docente del Profesorado en Enseñanza de la Lengua y la Literatura –ciclo–, de la Universidad Nacional de Río Negro. Los textos documentan decisiones en torno al registro de observaciones en campo, planificaciones de secuencias, métodos de análisis de prácticas, formas de reflexión sobre el trabajo docente. La sistematización de las prácticas, su registro y análisis permite reconocer una trama de significados negociados en las interacciones cotidianas, compartida en un aula virtual donde convergen múltiples escenarios del sistema educativo. El análisis de los textos revela un problema común: la dificultad de articulación conceptual y metodológica de objetos de enseñanza presentados de manera disgregada en los diseños curriculares vigentes que trazan la política lingüístico-educativa para nivel secundario en Argentina.
El Programa Nacional de Formación Docente (1999-2001) desde una investigación biográfico-narrativa
Authors: Aguirre Jonathan Ezequiel , Porta Luis
Published: May 2020

En los últimos años la formación docente ha sido cuestionada por las políticas nacionales y los discursos gubernamentales. Es por ello que se vuelve nodal recuperar experiencias que propiciaron el fortalecimiento de la formación docente desde marcos institucionales y pedagógicos alternativos. Compartiremos aquí los hallazgos de una investigación doctoral en relación a las potencialidades y tensiones que asumió la política del Programa Nacional de Formación Docente (1999-2001) en Institutos Superiores de Formación Docente de Argentina. Interpretaremos la política no solo desde el análisis de la normativa y documentos oficiales, sino, principalmente, desde su rostro más humano. Por ello, recuperaremos las voces de los sujetos que intervinieron en la planificación y puesta en marcha del programa desde un enfoque metodológico biográfico-narrativo. Dicha perspectiva otorga la posibilidad de habitar las políticas de formación desde las huellas que estas dejan en las biografías de los sujetos aportando nuevas indagaciones y abordajes al campo de la formación docente argentina.
Sound characterization and fine-scale spatial mapping of an estuarine soundscape in the southeastern USA
Authors: Mueller C , Monczak A , Soueidan J , McKinney B , Smott S , Mills T , Ji Y , Montie EW
Published: May 2020

Estuaries are areas known for biological diversity, and their soundscapes reflect the acoustic signals used by organisms to communicate, defend territories, reproduce, and forage in an environment that has limited visibility. These biological sounds may be rhythmic in nature, spatially heterogeneous, and can provide information on habitat quality. The goal of our study was to investigate the temporal and spatial variability of sounds in Chechessee Creek (Stns CC1 and CC2) and an adjacent saltwater impoundment (Great Salt Pond, GSP) in South Carolina, USA, from April to November 2016. Fixed recording platforms revealed that sound pressure levels (SPLs) were significantly higher in CC1 and CC2 compared to GSP. We detected some biological sounds in GSP (snapping shrimp genera Alpheus and Synalpheus, silver perch Bairdiella chrysoura, oyster toadfish Opsanus tau, spotted seatrout Cynoscion nebulosus, Atlantic croaker Micropogonias undulatus, and American alligator Alligator mississippiensis); however, biological sound was much more prevalent in CC1 and CC2. In Chechessee Creek, snapping shrimp, oyster toadfish, and spotted seatrout sounds followed distinct temporal rhythms. Using these data, we conducted spatial passive acoustic surveys in Chechessee Creek. We discovered elevated high frequency SPLs (representing snapping shrimp acoustic activity) near an anti-erosion wall, as well as increased low frequency SPLs (indicating spotted seatrout spawning aggregations) near the anti-erosion wall and at the mouth of Chechessee Creek. This study has demonstrated the utility of combining stationary and mobile recording platforms to detect acoustic hotspots of biological sounds.
Convergencia de COBIT e ISO 38500 en el Gobierno de Tecnologías de la Información
Authors: Robayo Jácome Darío Javier , Villarreal Morales Verónica De Las Mercedes
Published: May 2020

El objetivo del proyecto de investigación fue la implementación de un Modelo de Gestión y Gobierno de Tecnologías de la Información en la Universidad Estatal Amazónica (UEA). El desarrollo se inició con una revisión del estado del arte seguido de la aplicación del ciclo de vida y la técnica de cascada de metas que contempla ISACA a través de su marco de referencia COBIT 5; todo esto aplicado junto con los principios que se establecen en la norma internacional ISO 38500(2015), garantizó que la UEA aplique buenas prácticas de Gobierno de Tecnologías de la Información (TI) dotando de valor adicional a los procesos y estableciendo directrices para la mitigación de riesgos. Este modelo permitió a la UEA conjugar los objetivos de TI con los objetivos estratégicos institucionales, agregando valor y satisfaciendo las necesidades de cada dependencia a través de la administración de recursos (aplicaciones e infraestructura de TI) y la medición del desempeño de procesos relacionados con las TI. Al final se evidenció que la adopción de este Gobierno fue altamente satisfactoria ya que los resultados obtenidos en el corto plazo reflejan la optimización y mejor aprovechamiento de los recursos de TI.
Tiempo de ser diferente, tiempo de ser teacherpreneur y motivar estudiantes
Authors: Huaita Acha Cristian Abel
Published: May 2020

Uno de los factores determinantes del fracaso y deserción en la educación superior es la falta de motivación, un gran número de estudios ha hecho evidente que muchas instituciones educativas no generan ese vínculo especial entre el disfrute del estudiante en aula y las metodologías utilizadas por el docente. Ante la necesidad de  motivar estudiantes a niveles adecuados, la investigación encontró en el “teacherpreneur” o docente emprendedor, a  un modelo de docente que  respaldado por su metodología y preparación  procura que el estudiante forje actitud positiva hacia su futura profesión y se mantenga constantemente motivado. La investigación de tipo experimental y diseño pre experimental  buscó demostrar la influencia positiva del teacherpreneur sobre la motivación del estudiante y para tal fin se aplicó en Lima, Perú, en una escuela de negocios, un pre-test y un post-test, antes y después de recibir clases con teacherpreneurs, de tal forma que se compararon los niveles de motivación iniciales con los finales y se obtuvieron resultados categóricos que demostraron el grado significativo de influencia que un teacherpreneur ejerce sobre la motivación de sus estudiantes. Por ello la motivación es el eje impulsor que moviliza  toda conducta.
Las habilidades blandas como base del buen desempeño del docente universitario
Authors: Rodríguez Siu Jenny Liliana
Published: May 2020

El objetivo general de la investigación fue hallar la correlación entre las habilidades blandas y el desempeño docente en la Escuela de Posgrado de la Universidad Norbert Wiener. La metodología utilizada fue el enfoque cuantitativo, tipo no experimental transversal, nivel relacional, método descriptivo correlacional. Los resultados obtenidos comprueban una relación directa y significativa (d=0,601) entre las habilidades blandas y el desempeño docente, pudiéndose generalizar que aquellos docentes que poseen habilidades blandas son aquellos que tienen mejor desempeño laboral.  Se determinó que las cinco habilidades blandas evaluadas tienen una correlación con el desempeño docente, sin embargo, las de mayor relación significativa considerable con el desempeño docente son la responsabilidad (d=0,547), la adaptabilidad (d=0,651) y la gestión eficaz de la información (d=0,552); mientras que las habilidades de comunicación (d=0,374) y el desarrollo de los demás (d=0,487) arrojan una correlación positiva media.  Las habilidades blandas son las responsables de al menos el 70% del éxito laboral y debido a la fuerte correlación entre ambas variables, resulta imprescindible reformular el proceso de selección docente, así como los programas de inducción- capacitación con miras a incluir las habilidades blandas como indicador de peso en una contratación docente, que garantice una mejora en el desempeño.
Uncertainty Quantification Approach on Numerical Simulation for Supersonic Jets Performance
Authors: Cravero Carlo , De Domenico Davide , Ottonello Andrea
Published: May 2020

One of the main issues addressed in any engineering design problem is to predict the performance of the component or system as accurately and realistically as possible, taking into account the variability of operating conditions or the uncertainty on input data (boundary conditions or geometry tolerance). In this paper, the propagation of uncertainty on boundary conditions through a numerical model of supersonic nozzle is investigated. The evaluation of the statistics of the problem response functions is performed following ‘Surrogate-Based Uncertainty Quantification’. The approach involves: (a) the generation of a response surface starting from a DoE in order to approximate the convergent–divergent ‘physical’ model (expensive to simulate), (b) the application of the UQ technique based on the LHS to the meta-model. Probability Density Functions are introduced for the inlet boundary conditions in order to quantify their effects on the output nozzle performance. The physical problem considered is very relevant for the experimental tests on the UQ approach because of its high non-linearity. A small perturbation to the input data can drive the solution to a completely different output condition. The CFD simulations and the Uncertainty Quantification were performed by coupling the open source Dakota platform with the ANSYS Fluent® CFD commercial software: the process is automated through scripting. The procedure adopted in this work demonstrate the applicability of advanced simulation techniques (such as UQ analysis) to industrial technical problems. Moreover, the analysis highlights the practical use of the uncertainty quantification techniques in predicting the performance of a nozzle design affected by off-design conditions with fluid-dynamic complexity due to strong nonlinearity.
Structure of O-Polysaccharide and Lipid A of Pantoea Agglomerans 8488
Authors: Bulyhina Tetiana V. , Zdorovenko Evelina L. , Varbanets Ludmila D. , Shashkov Alexander S. , Kadykova Alexandra A. , Knirel Yuriy A. , Lushchak Oleh V.
Published: May 2020

The Pantoea agglomerans 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation. Lipid A was studied by electrospray ionization mass spectrometry (ESI-MS) and found to consist of hexa-, penta-, tetra- and tri-acylated species. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed the following structure of the O-polysaccharide repeating unit →3)-α-L-Rhap-(1→6)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-β-D-GlcNAcp-(1→. The LPS showed a low level of toxicity, was not pyrogenic, and reduced the adhesiveness index of microorganisms to 2.12, which was twofold less than the control. LPS modified by complex compounds of germanium (IV) and tin (IV) were obtained. It was found that six LPS samples modified by Sn compounds and two LPS samples modified by Ge compounds lost their toxic activity when administered to mice in a dose of LD50 (105 µg/mice or 5 mg/kg). However, none of the modified LPS samples changed their serological activity in an Ouchterlony double immunodiffusion test in agar.
Erratum: Pagonabarraga, J.; et al. A Spanish Consensus on the Use of Safinamide for Parkinson’s Disease in Clinical Practice. Brain Sci. 2020, 10, 176
Authors: Pagonabarraga Javier , Arbelo José Matías , Grandas Francisco , Luquin Maria-Rosario , Martínez Martín Pablo , Rodriguez-Oroz Maria Cruz , Valldeoriola Francesc , Kulisevsky Jaime
Published: May 2020

We would like to submit the following erratum to our recently published paper [...]
Massive Data Management and Sharing Module for Connectome Reconstruction
Authors: Yuan Jingbin , Zhang Jing , Shen Lijun , Zhang Dandan , Yu Wenhuan , Han Hua
Published: May 2020

Recently, with the rapid development of electron microscopy (EM) technology and the increasing demand of neuron circuit reconstruction, the scale of reconstruction data grows significantly. This brings many challenges, one of which is how to effectively manage large-scale data so that researchers can mine valuable information. For this purpose, we developed a data management module equipped with two parts, a storage and retrieval module on the server-side and an image cache module on the client-side. On the server-side, Hadoop and HBase are introduced to resolve massive data storage and retrieval. The pyramid model is adopted to store electron microscope images, which represent multiresolution data of the image. A block storage method is proposed to store volume segmentation results. We design a spatial location-based retrieval method for fast obtaining images and segments by layers rapidly, which achieves a constant time complexity. On the client-side, a three-level image cache module is designed to reduce latency when acquiring data. Through theoretical analysis and practical tests, our tool shows excellent real-time performance when handling large-scale data. Additionally, the server-side can be used as a backend of other similar software or a public database to manage shared datasets, showing strong scalability.
Gold Nanoparticle Mediated Multi-Modal CT Imaging of Hsp70 Membrane-Positive Tumors
Authors: Kimm Melanie A. , Shevtsov Maxim , Werner Caroline , Sievert Wolfgang , Zhiyuan Wu , Schoppe Oliver , Menze Bjoern H. , Rummeny Ernst J. , Proksa Roland , Bystrova Olga , Martynova Marina , Multhoff Gabriele , Stangl Stefan
Published: May 2020

Imaging techniques such as computed tomographies (CT) play a major role in clinical imaging and diagnosis of malignant lesions. In recent years, metal nanoparticle platforms enabled effective payload delivery for several imaging techniques. Due to the possibility of surface modification, metal nanoparticles are predestined to facilitate molecular tumor targeting. In this work, we demonstrate the feasibility of anti-plasma membrane Heat shock protein 70 (Hsp70) antibody functionalized gold nanoparticles (cmHsp70.1-AuNPs) for tumor-specific multimodal imaging. Membrane-associated Hsp70 is exclusively presented on the plasma membrane of malignant cells of multiple tumor entities but not on corresponding normal cells, predestining this target for a tumor-selective in vivo imaging. In vitro microscopic analysis revealed the presence of cmHsp70.1-AuNPs in the cytosol of tumor cell lines after internalization via the endo-lysosomal pathway. In preclinical models, the biodistribution as well as the intratumoral enrichment of AuNPs were examined 24 h after i.v. injection in tumor-bearing mice. In parallel to spectral CT analysis, histological analysis confirmed the presence of AuNPs within tumor cells. In contrast to control AuNPs, a significant enrichment of cmHsp70.1-AuNPs has been detected selectively inside tumor cells in different tumor mouse models. Furthermore, a machine-learning approach was developed to analyze AuNP accumulations in tumor tissues and organs. In summary, utilizing mHsp70 on tumor cells as a target for the guidance of cmHsp70.1-AuNPs facilitates an enrichment and uniform distribution of nanoparticles in mHsp70-expressing tumor cells that enables various microscopic imaging techniques and spectral-CT-based tumor delineation in vivo.
A Clonal NG2-Glia Cell Response in a Mouse Model of Multiple Sclerosis
Authors: Barriola Sonsoles , Pérez-Cerdá Fernando , Matute Carlos , Bribián Ana , López-Mascaraque Laura
Published: May 2020

NG2-glia, also known as oligodendrocyte precursor cells (OPCs), have the potential to generate new mature oligodendrocytes and thus, to contribute to tissue repair in demyelinating diseases like multiple sclerosis (MS). Once activated in response to brain damage, NG2-glial cells proliferate, and they acquire a reactive phenotype and a heterogeneous appearance. Here, we set out to investigate the distribution and phenotypic diversity of NG2-glia relative to their ontogenic origin, and whether there is a clonal NG2-glial response to lesion in an experimental autoimmune encephalomyelitis (EAE) murine model of MS. As such, we performed in utero electroporation of the genomic lineage tracer, StarTrack, to follow the fate of NG2-glia derived from single progenitors and to evaluate their response to brain damage after EAE induction. We then analyzed the dispersion of the NG2-glia derived clonally from single pallial progenitors in the brain of EAE mice. In addition, we examined several morphological parameters to assess the degree of NG2-glia reactivity in clonally-related cells. Our results reveal the heterogeneity of these progenitors and their cell progeny in a scenario of autoimmune demyelination, revealing the ontogenic phenomena at play in these processes.
Central Modulation of Selective Sphingosine-1-Phosphate Receptor 1 Ameliorates Experimental Multiple Sclerosis
Authors: Musella Alessandra , Gentile Antonietta , Guadalupi Livia , Rizzo Francesca Romana , De Vito Francesca , Fresegna Diego , Bruno Antonio , Dolcetti Ettore , Vanni Valentina , Vitiello Laura , Bullitta Silvia , Sanna Krizia , Caioli Silvia , Balletta Sara , Nencini Monica , Buttari Fabio , Stampanoni Bassi Mario , Centonze Diego , Mandolesi Georgia
Published: May 2020

Future treatments of multiple sclerosis (MS), a chronic autoimmune neurodegenerative disease of the central nervous system (CNS), aim for simultaneous early targeting of peripheral immune function and neuroinflammation. Sphingosine-1-phosphate (S1P) receptor modulators are among the most promising drugs with both “immunological” and “non-immunological” actions. Selective S1P receptor modulators have been recently approved for MS and shown clinical efficacy in its mouse model, the experimental autoimmune encephalomyelitis (EAE). Here, we investigated the anti-inflammatory/neuroprotective effects of ozanimod (RPC1063), a S1P1/5 modulator recently approved in the United States for the treatment of MS, by performing ex vivo studies in EAE brain. Electrophysiological experiments, supported by molecular and immunofluorescence analysis, revealed that ozanimod was able to dampen the EAE glutamatergic synaptic alterations, through attenuation of local inflammatory response driven by activated microglia and infiltrating T cells, the main CNS-cellular players of EAE synaptopathy. Electrophysiological studies with selective S1P1 (AUY954) and S1P5 (A971432) agonists suggested that S1P1 modulation is the main driver of the anti-excitotoxic activity mediated by ozanimod. Accordingly, in vivo intra-cerebroventricular treatment of EAE mice with AUY954 ameliorated clinical disability. Altogether these results strengthened the relevance of S1P1 agonists as immunomodulatory and neuroprotective drugs for MS therapy.