FIGURE 2 from Dabrafenib Alters MDSC Differentiation and Function by Activation of GCN2
Authors: Ciudad M. Teresa , Quevedo Rene , Lamorte Sara , Jin Robbie , Nzirorera Nadine , Koritzinsky Marianne , McGaha Tracy L. 
Published: March 2024

<p>PMN-MDSCs develop from monocytes and immature progenitors. <b>A,</b> Representative flow cytometry histogram of CFSE dilution in total monocytes (CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6C<sup>+</sup>) and total PMN (CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6G<sup>+</sup>) on days 1 and 4 in culture ± 1.5 µmol/L DAB. Day 4 panels show percent (%) of CFSE<sup>high</sup> cells. The purple shaded area shows PMN cells that remain quiescent and do not proliferate beyond the initial cycle on day 1. The green shaded area shows PMN cells that are highly proliferative. <b>B,</b> Representative flow cytometry contour plot of Ly6C and Ly6G expression in total PMNs gated as CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6G<sup>+</sup> on day 4 after differentiation ± 1.5 µmol/L DAB. Quiescent (purple) and proliferative (green) PMN cells described in A are overlaid for each condition. <b>C,</b> log<sub>2</sub> fold change (log<sub>2</sub>FC) of cell counts of total monocytes (CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6C<sup>+</sup>) and total PMNs (CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6G<sup>+</sup>) on day 4 normalized to the seeding counts of each lineage group at basal timepoint. MDSC were generated in presence of ± 1.5 µmol/L DAB. <i>P</i> values were determined by two-way ANOVA with Šidák correction post hoc-test. Significance considered <i>P</i> < 0.05. <b>D,</b> log<sub>2</sub>FC in proliferation measured by incorporation of DNA intercalating fluorescent dye on day 4, normalized to the fluorescence signal of cells at baseline. Sorted monocytes (CD3<sup>neg</sup>CD19<sup>neg</sup>CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6C<sup>+</sup>LyG6<sup>neg</sup>), PMN (CD3<sup>neg</sup>CD19<sup>neg</sup>CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6C<sup>med</sup>LyG6<sup>+</sup>) or progenitors (CD5<sup>neg</sup>CD11b<sup>neg</sup>CD19<sup>neg</sup> B220<sup>neg</sup>Gr-1<sup>neg</sup>TER119<sup>neg</sup>) were differentiated to MDSC ± 1.5 µmol/L DAB. Cells were sorted from fresh bone marrow and plated at the same cell density (2 × 10<sup>4</sup> cells/well). Bars show mean ± SD (<i>n</i> = 4). <i>P</i> values were determined by two-way ANOVA with Šidák correction post-test. Significance considered <i>P</i> < 0.05. <b>E,</b> Representative flow cytometry contour plot of Ly6C and Ly6G expression in CD11b<sup>+</sup>MHCII<sup>neg</sup> cells differentiated for 4 days. Initial cell source was either sorted bone marrow monocytes (CD3<sup>neg</sup>CD19<sup>neg</sup>CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6C<sup>+</sup>LyG6<sup>neg</sup>), bone marrow PMNs (CD3<sup>neg</sup>CD19<sup>neg</sup>CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6C<sup>med</sup>LyG6<sup>+</sup>) or bone marrow progenitors (CD5<sup>neg</sup>CD11b<sup>neg</sup>CD19<sup>neg</sup> B220<sup>neg</sup>Gr-1<sup>neg</sup>TER119<sup>neg</sup>). Gates show mean frequency. <b>F,</b> Frequency of total CD11b<sup>+</sup> cells during sorted progenitor differentiation, assessed by flow cytometry for each day of cell differentiation. Line represents mean ± SD (<i>n</i> = 4). <b>G,</b> Representative contour plot of Ly6C and Ly6G expression in CD11b<sup>+</sup>MHCII<sup>neg</sup> cells each day during sorted progenitor differentiation. Gates show mean frequency. Experiment repeated three times with similar results. <b>H,</b> Frequency of the granulocytic population CD11b<sup>+</sup>MHCII<sup>neg</sup>Ly6C<sup>+</sup>Ly6G<sup>+</sup> during progenitor differentiation, determined by flow cytometry for each day of cell differentiation. Bars indicate mean ± SD (<i>n</i> = 4). <i>P</i> values were determined by two-way ANOVA with Šidák correction post-test. Significance considered <i>P</i> < 0.05. For all panels, experiments were repeated three times with similar results. DAB = dabrafenib.</p>