bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring COVID-19 Vaccination Willingness in Italy: A Focus on Resident Foreigners and Italians Using Data from PASSI and PASSI d’Argento Surveillance Systems
Authors: Contoli Benedetta , Tosti Maria Elena , Asta Federica , Minardi Valentina , Marchetti Giulia , Casigliani Virginia , Scarso Salvatore , Declich Silvia , Masocco Maria
Published: January 2024

(1) The COVID-19 pandemic exacerbated health disparities, both between foreign and autochthonous populations. Italy was one of the European countries that was the most affected by the COVID-19 pandemic; however, only limited data are available on vaccine willingness. This study aims to assess the propensity of foreign and autochthonous populations residing in Italy to be vaccinated and the relative associated factors. (2) Data were collected and analysed from the two Italian surveillance systems, PASSI and PASSI d’Argento, in the period of August 2020–December 2021. The data include those of the Italian resident adult population over 18 years old. A multinomial logistic regression model, stratified by citizenship, was used to assess the associations of sociodemographic, health, and COVID-19 experience variables with vaccination attitudes. (3) This study encompassed 19,681 eligible subjects. Considering the willingness to be vaccinated, foreign residents were significantly less certain to get vaccinated (49.4% vs. 60.7% among Italians). Sociodemographic characteristics, economic difficulties, and trust in local health units emerged as factors that were significantly associated with vaccine acceptance. Having received the seasonal flu vaccine was identified as a predictor of COVID-19 vaccine acceptance among foreign and Italian residents. (4) This study underscores the significance of tailoring interventions to address vaccine hesitancy based on the diverse characteristics of foreign and Italian residents. This research offers practical insights for public health strategies, highlighting the importance of tailored educational campaigns, improved communication, and nuanced interventions to enhance vaccine acceptance and uptake within both populations.
COVID-19 Vaccination Coverage and Associated Factors in Patients with Multiple Sclerosis
Authors: Hernández-García Ignacio , Rodríguez-Montolio Joana , Almeida-Zurita Monserrath , Cheli-Gracia Dionisio , Sahuquillo Belén del Moral , Aibar-Remón Carlos , Garcés-Redondo Moisés
Published: January 2024

Our objective was to know the COVID-19 vaccination coverage in multiple sclerosis (MS) patients and its factors associated. A retrospective cohort study was carried out. Patients seen at the MS unit of the University Clinical Hospital of Zaragoza between 2017 and 2021 were included. Variables were obtained by reviewing the specialized and primary care records. Associations between receiving COVID-19 full primo-vaccination, as well as one booster dose since autumn 2022, and the other variables were analyzed using bivariate analysis and multiple logistic regression models. Of the 359 included patients, 90.3% received the COVID-19 full primo-vaccination. Having been born in Spain (aOR = 3.40) and having received the 2020–2021 influenza vaccine (aOR = 6.77) were associated with receiving the COVID-19 full primo-vaccination. Vaccination with a COVID-19 booster dose was detected in 141 patients (39.3%). Sex (man) (aOR = 2.36), age (60 years or over) (aOR = 6.82), type of MS (Primary Progressive/Secondary Progressive) (aOR = 3.94), and having received the 2022–2023 influenza vaccine (aOR = 27.54) were associated with receiving such a booster dose. The COVID-19 booster dose was administered at the same time as the 2022–2023 influenza vaccine in 57.8% (67/116) of the patients vaccinated with both vaccines. The COVID-19 full primo-vaccination coverage is higher than in other countries. However, the decrease in vaccination coverage with the booster dose makes it necessary to develop strategies to improve it that are not limited to administering the flu vaccine together with the COVID-19 booster dose. Such strategies should be in focus, especially for women under 60 years of age.
Lung IL-17A-Producing CD4+ T Cells Correlate with Protection after Intrapulmonary Vaccination with Differentially Adjuvanted Tuberculosis Vaccines
Authors: Stewart Erica L. , Counoupas Claudio , Quan Diana H. , Wang Trixie , Petrovsky Nikolai , Britton Warwick J. , Triccas James A.
Published: January 2024

Tuberculosis (TB), caused by Mycobacterium tuberculosis, results in approximately 1.6 million deaths annually. BCG is the only TB vaccine currently in use and offers only variable protection; however, the development of more effective vaccines is hindered by a lack of defined correlates of protection (CoP) against M. tuberculosis. Pulmonary vaccine delivery is a promising strategy since it may promote lung-resident immune memory that can respond rapidly to respiratory infection. In this study, CysVac2, a subunit protein previously shown to be protective against M. tuberculosis in mouse models, was combined with either Advax® adjuvant or a mixture of alum plus MPLA and administered intratracheally into mice. Peripheral immune responses were tracked longitudinally, and lung-local immune responses were measured after challenge. Both readouts were then correlated with protection after M. tuberculosis infection. Although considered essential for the control of mycobacteria, induction of IFN-γ-expressing CD4+ T cells in the blood or lungs did not correlate with protection. Instead, CD4+ T cells in the lungs expressing IL-17A correlated with reduced bacterial burden. This study identified pulmonary IL-17A-expressing CD4+ T cells as a CoP against M. tuberculosis and suggests that mucosal immune profiles should be explored for novel CoP.
Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies
Authors: Solodkov Pavel P. , Najakshin Alexander M. , Chikaev Nikolai A. , Kulemzin Sergey V. , Mechetina Ludmila V. , Baranov Konstantin O. , Guselnikov Sergey V. , Gorchakov Andrey A. , Belovezhets Tatyana N. , Chikaev Anton N. , Volkova Olga Y. , Markhaev Alexander G. , Kononova Yulia V. , Alekseev Alexander Y. , Gulyaeva Marina A. , Shestopalov Alexander M. , Taranin Alexander V.
Published: January 2024

The emergence of SARS-CoV-2 mutant variants has posed a significant challenge to both the prevention and treatment of COVID-19 with anti-coronaviral neutralizing antibodies. The latest viral variants demonstrate pronounced resistance to the vast majority of human monoclonal antibodies raised against the ancestral Wuhan variant. Less is known about the susceptibility of the evolved virus to camelid nanobodies developed at the start of the pandemic. In this study, we compared nanobody repertoires raised in the same llama after immunization with Wuhan’s RBD variant and after subsequent serial immunization with a variety of RBD variants, including that of SARS-CoV-1. We show that initial immunization induced highly potent nanobodies, which efficiently protected Syrian hamsters from infection with the ancestral Wuhan virus. These nanobodies, however, mostly lacked the activity against SARS-CoV-2 omicron-pseudotyped viruses. In contrast, serial immunization with different RBD variants resulted in the generation of nanobodies demonstrating a higher degree of somatic mutagenesis and a broad range of neutralization. Four nanobodies recognizing distinct epitopes were shown to potently neutralize a spectrum of omicron variants, including those of the XBB sublineage. Our data show that nanobodies broadly neutralizing SARS-CoV-2 variants may be readily induced by a serial variant RBD immunization.
Effect of Platform Type on Clinical Efficacy of SARS-CoV-2 Vaccines in Prime Vaccination Settings: A Systematic Review and Meta-Regression of Randomized Controlled Trials
Authors: Goryaynov Sergey , Gurova Olesya
Published: January 2024

This systematic review investigated the association between platform type and the clinical efficacy of SARS-CoV-2 vaccines using the meta-regression of randomized controlled trials to compare the rates of the first appearance of symptomatic COVID-19 on the platforms. The trial search was conducted using PubMed, ClinicalTrials.gov, and the EU Clinical Trials Register. The main selection criteria included: non-active control, immunocompetent individuals without previous vaccination, and a low risk of bias. The platform effect was summarized with an incidence rate ratio (IRR) and a 95% confidence interval for every platform category against the reference. IRR was obtained by random-effect meta-regression with adjustment for confounding by effect modifiers. The analysis was conducted in per-protocol (PP) and modified intention-to-treat (mITT) sets. Six vaccine types with 35 trials were included. Vector vaccines were a reference category. In the PP set, rates of symptomatic COVID-19 on mRNA and protein subunit vaccines were significantly lower than on the vector: IRR = 0.30 [0.19; 0.46], p = 0.001 and 0.63 [0.46; 0.86], p = 0.012, respectively. There was no difference for inactivated and virus-like particle vaccines compared to the vector: IRR = 0.98 [0.71; 1.36], p = 0.913 and 0.70 [0.41; 1.20], p = 0.197, respectively. The rate of cases on DNA vaccines was significantly higher than that on the vector: IRR = 2.58 [1.17; 5.68], p = 0.034. Results for the mITT set were consistent. Platform type is an effect modifier of the clinical efficacy of SARS-CoV-2 vaccines.
How Does the Government Guide Marine Resource Enterprises in China to Improve Their Business Performance? A Path Analysis Based on DEA-fsQCA
Authors: Wang Juying , Chen Jialu
Published: January 2024

Policy guidance is a key driving force for improving the business performance of marine resource enterprises. This study establishes a DEA-fsQCA model, selects 42 listed marine resource enterprises as samples, analyzes the business performance improvement paths of marine resource enterprises, and proposes relevant policy recommendations for the government to guide marine resource enterprises to improve their business performance. The result shows that there are three different path models for the high business performance of marine resource enterprises based on their scale and property-right attributes: the “private green innovation” type, the “private green concentration” type, and the “state-owned incentive decentralized” type. According to the research results, this study suggests that, in the process of promoting the improvement of the business performance of marine resource enterprises, the Chinese government should promote the green development of enterprises, stimulate the technological innovation vitality of private marine resource enterprises, optimize enterprise executive incentive policies, and deepen the reform of mixed ownership in state-owned enterprises. Compared with previous studies, this article presents a fresh perspective on researching marine resource enterprises from a macro perspective and constructs a policy system for improving the business performance of different types of marine resource enterprises, providing valuable reference and guidance for the high-quality development of marine resource enterprises and the overall marine economy.
Unraveling Zooplankton Diversity in a Pre-Alpine Lake: A Comparative Analysis of ZooScan and DNA Metabarcoding Methods
Authors: Vogelmann Christian , Barco Andrea , Knust Jean-Michel , Stibor Herwig
Published: January 2024

Zooplankton, integral to aquatic ecosystems, face diverse environmental influences. To comprehend their dynamics, critical for ecological insights and fisheries management, traditional morphological analysis proves laborious. Recent advances include automated systems like ZooScan and DNA metabarcoding. This study examines two methods on the same samples to identify similarities and dependencies between them, potentially reducing the required workload and enhancing the quality of the results. Ten Lake Starnberg vertical tows in September 2021 provided zooplankton samples preserved in ethanol. Subsamples underwent ZooScan morphological identification and subsequent DNA metabarcoding. High concordance between ZooScan counts and DNA reads (86.8%) was observed, while biomass calculations from body length (major axis) and equivalent spherical diameter (ESD) showed slightly lower agreement (78.1% and 79.6%, respectively). Linear regression analysis revealed a correlation between counts and DNA reads (r2 = 0.59). This study underscores the complementary strengths and limitations of ZooScan and DNA metabarcoding for zooplankton analysis. ZooScan aids biomass estimation and morphological differentiation, whereas DNA metabarcoding offers superior taxonomic resolution and low-abundance taxon detection. Combining both methods on the same sample enhances understanding and facilitates future advanced analyses.
Impact of COVID-19 vaccination on the severity of the course and outcomes of the disease in hospitalized patients in St. Petersburg
Authors:   North-Western State Medical University named after I. I. Mechnikov of the Ministry of Health of Russia, St. Petersburg, Russia, Nechaev V. V. , Zaguzov V. S. ,   St. Petersburg Pasteur Institute of Rospotrebnadzor, St. Petersburg, Russia; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, St. Petersburg, Russia, Lyalina L. V. ,   North-Western State Medical University named after I. I. Mechnikov of the Ministry of Health of Russia, St. Petersburg, Russia; St. Petersburg Pasteur Institute of Rospotrebnadzor, St. Petersburg, Russia
Published: January 2024

Vaccination against infection caused by the SARS-CoV-2 virus, despite the problems of a biological and social nature, remains the main preventive measure to prevent the spread of this infection. The aim is to assess the effect of COVID-19 vaccine prophylaxis on the incidence of severe forms and deaths among hospitalized patients. A retrospective epidemiological analysis of official data on 13,778 patients hospitalized in the main repurposed hospitals of the city of St. Petersburg was carried out, of which 973 people had a fatal disease. The frequency of pneumonia and deaths among vaccinated and unvaccinated patients was analyzed. Correlation and regression analysis was used to determine the nature of the relationship between these factors. An analysis of the incidence of pneumonia and deaths in patients with different vaccination status showed their statistically significant predominance among unvaccinated patients (p < 0.05). Quantitative fluctuations in deaths in 2021–2022 were established due to the beneficial effect of preventive vaccinations against COVID-19, expressed in a decrease in hospital mortality rates. The greatest reduction in mortality was achieved in the group of vaccinated patients in the absence of pneumonia. The risk of adverse outcomes was highest in the presence of pneumonia and the absence of vaccinations. A significant favorable factor was the vaccination coverage of hospitalized patients. COVID-19 vaccine prophylaxis has a positive effect on both the severity of this infection and its outcomes. However, a critical factor in this case is the degree of immunization of the population.
Epidemiological features of viral infections of the upper respiratory tract in the Voronezh Region
Authors:   Federal State Budgetary Educational Institution of Higher Education «Voronezh State Medical University named after N. N. Burdenko» of the Ministry of Health of the Russian Federation, Voronezh, Russia, Stepkin Yu. I. , Mamchik N. P. ,   Federal State Budgetary Educational Institution of Higher Education «Voronezh State Medical University named after N. N. Burdenko» of the Ministry of Health of the Russian Federation, Voronezh, Russia, Usacheva L. P. ,   Federal State Budgetary Educational Institution of Higher Education «Voronezh State Medical University named after N. N. Burdenko» of the Ministry of Health of the Russian Federation, Voronezh, Russia, Dolgopolova T. V. ,   Budgetary Healthcare Institution of the Voronezh Region «Voronezh City Emergency Hospital No. 1», Kameneva O,V. ,   Federal State Budgetary Educational Institution of Higher Education «Voronezh State Medical University named after N. N. Burdenko» of the Ministry of Health of the Russian Federation, Voronezh, Russia
Published: January 2024

Upper respiratory tract infections occupy an important place in the group of infectious diseases, determining significant damage to the economy of the Russian Federation, which is confirmed by data from state reports «On the state of sanitary and epidemiological welfare of the population of the Russian Federation». The aim of the study was to assess the epidemic situation of upper respiratory tract infections in the Voronezh region. In the course of the study, it was found that upper respiratory tract infections have been steadily determining epidemic problems in the Voronezh Region for many years. The presence of changes in indicators within the year, depending on the season, allows you to distribute efforts to prepare for the next epidemic upsurge, including specific prevention of influenza and other diseases against which vaccines have been developed and registered. The unified mechanism of transmission of the described infections indicates that the implementation of non-specific prevention, including timely identification and isolation of patients, air purification and disinfection, etc. It will help to reduce the spread of pathogens in organized groups.
Effects of vitamin D on the incidence and severity of COVID-19
Authors:   High Technology Park, Khanty-Mansiysk, Russia, Krivosheev V. V. , Nikitina L. Yu. ,   National Medical Research Center, specializing in pulmonology, Moscow, Russia, Kozlovskiy I. V. ,   BU «District Clinical Hospital», Khanty-Mansiysk, Russia, Fedorov A. V. ,   High Technology Park, Khanty-Mansiysk, Russia
Published: January 2024

In the fall of 2023, infection of a number of European countries population with coronavirus intensified, with the most noticeable increase in incidence observed in the Russian Federation. Patients infected with SARS-CoV-2 usually have a low level and high prevalence of vitamin D deficiency. Data from measurements of vitamin D levels in the municipalities of the Khanty-Mansiysk autonomous Okrug — Ugra, compared with information from the department of health of the autonomous okrug based on the results of 2021, suggest that in the Khanty-Mansi autonomous Okrug — Ugra, the average duration of one case of disability due to illness with a diagnosis of COVID-19 is directly proportional to the prevalence of severe vitamin D deficiency. Our data characterizing the positive effect of a high level of vitamin D in the blood serum of a patient infected SARS-CoV-2, on the body’s ability to recover, is confirmed in foreign clinical practice, as evidenced by the results of our statistical calculations. In the Russian Federation, the dependence of the overall incidence of COVID-19 is directly proportional to the geographical latitude of the territory, and the relationship b etween the parameters is extremely high (p = 0.0008 in 2021 and p < 0.000001 in 2022), which, along with other reasons, suggests the effect of ultraviolet radiation level decrease. Vitamin D levels are one of the key factors in the incidence of COVID-19, as it has a major impact on its duration, more severe lung damage, and higher mortality in patients infected with SARS-CoV-2. Clinical studies by domestic and foreign scientists have shown that severe vitamin D deficiency [25(OH)D < 10 ng/ml] in patients infected with SARS-CoV-2 can be considered a predictor of death. During the COVID-19 pandemic, enormous positive experience has been gained in the use of vitamin D as a therapeutic agent to reduce the severity of the disease and the level of hospital mortality, however, in order to prepare informed recommendations for taking medications and identify severe vitamin D deficiency, mandatory measurement of 25(OH)D levels in patients is required in patients diagnosed with SARS-CoV-2 infection.
Hygienic problem of providing university students with educational furniture
Authors:   Federal State Budgetary Educational Institution of Higher Education «Omsk State Medical University» of the Russian Federation Ministry of Health, Omsk, Russia, Vaskovskaya Yu. S. , Gudinova Zh. V. ,   Federal State Budgetary Educational Institution of Higher Education «Omsk State Medical University» of the Russian Federation Ministry of Health, Omsk, Russia, Zhernakova G. N. ,   Federal State Budgetary Educational Institution of Higher Education «Omsk State Medical University» of the Russian Federation Ministry of Health, Omsk, Russia, Schreider A. Yu. ,   Federal State Budgetary Educational Institution of Higher Education «Omsk State Medical University» of the Russian Federation Ministry of Health, Omsk, Russia, Zavyalova A. V. ,   Federal State Budgetary Educational Institution of Higher Education «Omsk State Medical University» of the Russian Federation Ministry of Health, Omsk, Russia
Published: January 2024

The primary task of state policy is to preserve and improve the health of young people, which requires compliance with the hygienic regulations of the educational process and the educational environment, including educational furniture. The present study substantiates hygienic recommendations for the selection of educational furniture at the university, taking into account the sexual structure of students. 75 % of girls and 25 % of boys study at Omsk State Medical University. To ensure the correct working position, two thirds (65 %) of students need furniture No. 5, 20 % of students need furniture No. 6, 11 % need furniture No. 4, and 4 % of students taller than 185 cm need furniture No. 7. Thus, the inclusion of furniture No. 7 in the regulations of sanitary rules is required. The results of the conducted research can provide significant assistance to the university administration in purchasing educational furniture.
Riconciliazione tra Davide e assalonne, padre e figlio analisi narrativa e valutazione del ruolo di ioab in 2 sam 13,39 – 14,33
Authors: Kljajić Stipo
Published: January 2024

L’articolo cerca di far luce sul ruolo di Ioab, il comandante di lunga data dell’esercito di Davide e uno degli uomini più vicini a Davide e allo stesso tempo uno dei personaggi più controversi dei Libri di Samuele, nel processo del ritorno di Assalonne, figlio di Davide e suo potenziale successore, dall’esilio (in cui si ritrovò dopo aver ucciso il fratello Amnòn dopo che questi aveva violentato la loro sorella Tamar ed era rimasto impunito), e in un altrettanto complesso processo di riconciliazione tra Davide e Assalonne, padre e figlio, re e futuro re. Come in tutta la Storia della successione (2 Sam 9 – 20; 1 Re 1 – 2), anche in questo racconto (2 Sam 13,39 – 14,33) il rapporto tra Ioab e Davide è piuttosto complesso, anzi ambivalente, motivo per cui l’autore, nella prima parte dell’articolo, analizza il ruolo di Ioab nella narrazione (attraverso sei fasi di analisi narrativa: azioni preliminari, ossia delimitazione della narrazione e problemi di traduzione; forme verbali; tempo e ritmo; azione; tecniche narrative; presentazione dei personaggi) per poi, nella seconda parte, presentare e valutare il ruolo di Ioab nella vicenda, utilizzando, oltre ai risultati dell’analisi narrativa, altri metodi e approcci biblici. Infine, nella conclusione, presenta brevemente i risultati dell’analisi del ruolo di Ioab nella narrazione, cioè nel racconto, e questi, in breve, rivelano il ruolo decisivo di Ioab non solo nel ritorno di Assalonne dall’esilio e nella ciliazione con il re, ma anche nella complessa Storia della successione, poiché Assalonne, Davide, e anche Ioab, che hanno avuto ruoli chiavi nelle vicende passate, avranno tali ruoli anche nelle vicende che seguiranno, strettamente connesse con le prime.
Bifunctional Tumor-Targeted Bioprobe for Phothotheranosis
Authors: Park Hae Sang , Yokomizo Shinya , Wang Haoran , Manganiello Sophia , Monaco Hailey , McDonnell Rose , Kim Hajin Joanne , Rho Jiyun , Ahn Sung , Jung Harry , Kang Homan , Bao Kai , Kashiwagi Satoshi , Choi Hak Soo
Published: January 2024

Background:
Near-infrared (NIR) phototheranostics provide promising noninvasive imaging and treatment for head and neck squamous cell carcinoma (HNSCC), capitalizing on its adjacency to skin or mucosal surfaces. Activated by laser irradiation, targeted NIR fluorophores can selectively eradicate cancer cells, harnessing the power of synergistic photodynamic therapy and photothermal therapy. However, there is a paucity of NIR bioprobes showing tumor-specific targeting and effective phototheranosis without hurting surrounding healthy tissues.
Methods:
We engineered a tumor-specific bifunctional NIR bioprobe designed to precisely target HNSCC and induce phototheranosis using bioconjugation of a cyclic arginine–glycine–aspartic acid (cRGD) motif and zwitterionic polymethine NIR fluorophore. The cytotoxic effects of cRGD-ZW800-PEG were measured by assessing heat and reactive oxygen species (ROS) generation upon an 808-nm laser irradiation. We then determined the in vivo efficacy of cRGD-ZW800-PEG in the FaDu xenograft mouse model of HNSCC, as well as its biodistribution and clearance, using a customized portable NIR imaging system.
Results:
Real-time NIR imaging revealed that intravenously administered cRGD-ZW800-PEG targeted tumors rapidly within 4 h postintravenous injection in tumor-bearing mice. Upon laser irradiation, cRGD-ZW800-PEG produced ROS and heat simultaneously and exhibited synergistic photothermal and photodynamic effects on the tumoral tissue without affecting the neighboring healthy tissues. Importantly, all unbound bioprobes were cleared through renal excretion.
Conclusions:
By harnessing phototheranosis in combination with tailored tumor selectivity, our targeted bioprobe ushers in a promising paradigm in cancer treatment. It promises safer and more efficacious therapeutic avenues against cancer, marking a substantial advancement in the field.
Cell Surface Modification-Mediated Primary Intestinal Epithelial Cell Culture Platforms for Assessing Host–Microbiota Interactions
Authors: Sittipo Panida , Anggradita Laurensia Danis , Kim Hyunbum , Lee Chanyoung , Hwang Nathaniel S. , Lee Yun Kyung , Hwang Yongsung
Published: January 2024

Background:
Intestinal epithelial cells (IECs) play a crucial role in regulating the symbiotic relationship between the host and the gut microbiota, thereby allowing them to modulate barrier function, mucus production, and aberrant inflammation. Despite their importance, establishing an effective ex vivo culture method for supporting the prolonged survival and function of primary IECs remains challenging. Here, we aim to develop a novel strategy to support the long-term survival and function of primary IECs in response to gut microbiota by employing mild reduction of disulfides on the IEC surface proteins with tris(2-carboxyethyl)phosphine.
Methods:
Recognizing the crucial role of fibroblast-IEC crosstalk, we employed a cell surface modification strategy, establishing layer-to-layer contacts between fibroblasts and IECs. This involved combining negatively charged chondroitin sulfate on cell surfaces with a positively charged chitosan thin film between cells, enabling direct intercellular transfer. Validation included assessments of cell viability, efficiency of dye transfer, and IEC function upon lipopolysaccharide (LPS) treatment.
Results:
Our findings revealed that the layer-by-layer co-culture platform effectively facilitates the transfer of small molecules through gap junctions, providing vital support for the viability and function of primary IECs from both the small intestine and colon for up to 5 days, as evident by the expression of E-cadherin and Villin. Upon LPS treatment, these IECs exhibited a down-regulation of
Villin
and tight junction genes, such as
E-cadherin
and
Zonula Occludens-1
, when compared to their nontreated counterparts. Furthermore, the transcription level of
Lysozyme
exhibited an increase, while
Mucin 2
showed a decrease in response to LPS, indicating responsiveness to bacterial molecules.
Conclusions:
Our study provides a layer-by-layer-based co-culture platform to support the prolonged survival of primary IECs and their features, which is important for understanding IEC function in response to the gut microbiota.
ОЦІНКА МІТОТИЧНОЇ АКТИВНОСТІ В КРИПТАХ ЕПІТЕЛІЮ ТОНКОГО КИШЕЧНИКУ CALIDRIS ALPINA (LINNAEUS, 1758)
Authors: Ликова І. О. , Харченко Л. П.
Published: January 2024

В даній роботі проаналізовано інтенсивність регенераційних процесів епітелію в тонкому кишечнику побережника чорногрудого Calidris alpina (Linnaeus, 1758) під час міграційних зупинок. Активне живлення птахів під час міграційних зупинок сприяє підвищенню метаболічних процесів в організмі птахів. Процеси всмоктування поживних речовин прискорюються, що призводить до швидкого виснаження облямованих ентероцитів на верхівці кишкових ворсинок. Наслідком цього є підвищення темпів регенерації кишкового епітелію. На сьогодні регенераційні потенції кишкового епітелію добре вивчені у ссавців та частково у домашніх видів птахів, на диких видах птахів такі дослідження не проводились. В роботі наведено коротку характеристику гістологічної будови стінки тонкого кишечнику побережника чорногрудого та описано цитологічний склад кишкових крипт. Вивчення мікроструктури крипт тонкого кишечнику C. alpina показало, що на відміну від епітелію ворсинок, до складу крипт входять не лише облямовані стовпчасті ентероцити та ендокриноцити, а також стовбурові клітини, клітини-попередниці, клітини, що диференціюються на різних стадіях розвитку та клітини Панета. Проліферативна активність крипт визначена шляхом обрахунку мітотичного індексу (МІ) в криптах тонкого кишечнику C. alpinа, за оцінкою якого визначено відділ з найвищими показниками МІ в криптах. За результатами проведених досліджень установлено, що в криптах дванадцятипалої кишки МІ збільшується в дистальному напрямку і в каудальному відділі дорівнює 21.35%. Найвищі показники МІ зафіксовано в криптах краніальної частини порожньої кишки –25.65%. В каудальному відділі клубової кишки відмічена тенденція до зниження показників МІ в криптах до 16.5%. Оцінка МІ в клітинах крипт тонкого кишечнику C. alpina показала, що всі відділи тонкого кишечнику мають високі показники МІ в криптах. Найвищі значення МІ епітеліоцитів крипт було зафіксовано у порожній кишці, що свідчить про інтенсифікацію процесів травлення в цьому відділі і високу проліфераційну активність його епітелію. Отримані результати є ще одним свідченням пластичності травної системи птахів-мігрантів.
ДИНАМІКА ЧИСЕЛЬНОСТІ ГРАКА CORVUS FRUGILEGUS L. У МИКОЛАЇВСЬКІЙ ОБЛАСТІ В ХХІ СТОРІЧЧІ
Authors: Редінов К. О. , Петрович З. О.
Published: January 2024

Облік колоній грака проведено маршрутним методом у 2016–2021 рр. Кількість гнізд в колонії визначали проїжджаючи повз них на невеликій швидкості або обходячи їх пішки. Обстежено більшу частину території області. Також використано дані з сайту eBird та повідомлення колег і деяких аматорів. На підставі отриманих даних зроблено експертну оцінку чисельності.

Для порівняння використано дані зібрані в 2008–2010 рр. таким же методом і отримані у 1984 р. методом анкетування. Вже у 1991–2010 рр. прослідковано зменшення чисельності та зміни у просторовому розподілі колоній грака. У 2008–2010 рр. виявлено 36 колоній (13202 гнізд), оціночна чисельність виду не перевищувала 20 тис. пар. Більшість птахів гніздились на півдні області. Після 2010 р. виявлено лише чотири нові колонії. Більшість відомих колоній зникли або зменшився їх розмір. У 2016–2023 рр. обліковано 20 колоній (3990 гнізд). Станом на 2020–2023 рр. чисельність грака, за нашою оцінкою, не перевищує 5 тис. пар. За останні 15 років чисельність грака скоротилася щонайменше в 4 рази, а порівняно з 1980-ми роками. – 11 разів. Розмір колонії становив у 2008–2010 рр. (n = 36) – 366,7 ± 352,2 (Ме = 208,5) (40–1210), а в 2016–2023 р. (n = 20) – 199,5 ± 143,6 (Ме = 180) (30–600) гнізд. Різниця між розмірами колоній (U-критерій Манна-Уітні) не є статистично значущою: U = 278; P > 0,001).

Важливими факторами, що спричинили зникнення колоній грака у ХХІ ст. є істотні зміни в сільському господарюванні, відлякування птахів та розселення великого яструба. Навіть у 2011–2023 рр. відомі випадки вигнання птахів з колонії під час гніздування шляхом відлякування або спилювання дерев з гніздами. Падіння чисельності виду в Миколаївській області, по-суті, відображає загальноєвропейську тенденцію.
Towards sustainable purchase: the effect of social responsibility, innovativeness and knowledge of natural cosmetics purchasing consumers’ intentions
Authors: Witek Lucyna , Kędzia Grażyna , Staniec Iwona
Published: January 2024

The article aims to broaden the understanding of factors affecting natural cosmetics purchasing behaviours and specifically, to specify the role social responsibility, consumers' innovativeness and knowledge play in shaping purchasing intentions for these products. The study uses the extended theory of planned behaviour as its theoretical framework and is based on theoretical support and suggested modification indices. The study applies a quantitative methodology which collects survey data from Ukraine and Poland. It was analysed using Structural Equation Modelling. Results reveal that attitude towards purchasing natural cosmetics, social norms, consumers' innovativeness and consumer’s natural cosmetics knowledge all have statistically significant and positive impacts on the purchase intention towards natural cosmetic products. This study contributes to the literature by incorporating other variables into the TPB model. It provides new insights and constitutes a useful step forward in the understanding of consumers’ behaviours towards natural cosmetics.
Is seniors' tourism behavior related to sustainable tourism?
Authors: Przybysz Klaudia , Roszko-Wójtowicz Elżbieta , Stanimir Agnieszka
Published: January 2024

The article addresses two global challenges in the realm of tourism participation: sustainable development and an ageing society. Our research aimed to determine whether European seniors engage in tourism and whether the factors influencing tourist destinations are related to sustainable tourism. To achieve this, we utilized correspondence analysis with representative Eurobarometer data. Our findings confirm that seniors actively participate in tourism. The majority of seniors in the European Union frequently express their heightened concern for health and safety measures when travelling in the post-COVID-19 era.Moreover, the analysis identified the countries where seniors actively pursue sustainable development goals during their tourism activities. Future research should focus on understanding the decision-making processes of seniors when choosing tourist destinations and accessing tourism offers. These results provide a foundation for designing tourism offerings that cater to the current and future needs of seniors.
Study of Korean Studies in China from the Perspective of Area studies
Authors:   Korean Association of Regional Sociology, Zhang Guoqiang , Xu Qianqian
Published: January 2024

This article investigates the research prospect of Korean studies after area studies have been newly established as a discipline in China. This study quantitively analyzes the current research situations and research fields of Korean studies based on the examination of the Korean Studies-related research projects sponsored by National Social Science Funding which is the representative national research project in China, and the Korean Studies-related articles published on the journals in China. The findings are: 1) the foundations of Korean studies in China are expanding;2) both the quantity and quality of Korean studies in China are increasingly growing. It is thus shown that more attentions have been paid to East Asian studies, China Theory, China perspective, comparative study and bilateral relation study. However, as an interdisciplinary field, there is a lack of interdisciplinary collaboration and cooperation between Chinese and Korean researchers. It is thus suggested that more exchanges and cooperations should be needed among the researchers from different disciplines, different areas and different nations in order for the problems to be solved.
Determinants of depressive symptoms and suicidal ideation among young adults in Jeollabuk-do: Focusing on sex-differential patterns before and during the COVID-19 pandemic
Authors:   Korean Association of Regional Sociology, Kim Jae-Woo
Published: January 2024

The purpose of the present study is to examine sex differences in determinants of depression and suicidal ideation risks among young adults in Jeollabuk-do before and after the COVID-19 outbreak, with focused on variables related to socioeconomic status and social isolation. Towards this end, we used the sample of twenties and thirties from the Korean Community Health Survey data in 2017 and 2021. During the pandemic, we found 1) the greater gradient in moderate or stronger depressive symptoms along educational attainment and the principle economic status regardless of gender and in suicidal ideation among economically inactive women during the pandemic; 2) the wider difference in the risk of suicidal thought between the married and the single due to divorce, separation, or non-marriage; and 3) solitary living and residing in urban areas as independent risk factors for both depression and suicidal ideation among women and for depression regardless of sex, respectively. In addition, good environmental amenities in neighborhood serves as a resource factor for mental health, whereas high risk drinking is more likely to suppress the association between depression and suicidal ideation among women than among men. From the perspective of the internal inequalities within the same group of young adults, we provide overarching principles to overcome their health disparities for local policy makers and practitioners based on our main findings.
Menurunkan Waktu Penggantian Tabung Gas Welding (GMAW) Menggunakan Metode Single Minute Exchange Die (SMED) di Politeknik Astra
Authors: Aisa Neilinda Novita , Prahara Rahayu Budi , Sadewa Eduardus Dimas Arya , Wahyudi Wahyudi , Wicaksono Andreadie
Published: January 2024

Politeknik Astra is a high vocational education institution that have several laboratories. Welding laboratoy has longest preparation time compare to another laboratories. Gas cylinder changes process in Gas Metal Arc Welding (GMAW) takes 29.2 minutes, which is the longest preparation time so the welding training is hinder by it. Previous condition of  gas instalation is, every GMAW machine equiped with one  gas cylinder,  so if the cylinder is run out of  gas, it have to changes and potentially it could be done more than one machine. The aim of this research is to reduce gas cylinder changes time in welding laboratory using single minute exchange of dies methode (SMED). SMED is one of lean manufacturing metodes that able to identified and reduce or eliminate time waste by separated internal setup and external setup. Using SMED metods, gas cylinder changes activities are identified then divided by internal and external setup. The activities that have no effect to gas flow from cylinder to machine are convert from internal to external setup. For the final, external setup are simplified using 5W2H tools. The solution that reserearcher found using SMED method is to built a centralized gas system with one cylinder as an active gas supply for whole GMAW machine and the other one as  a backup cylinder, in stead of install  gas cylinder on every GMAW machine. The result of this research is setup time for gas cylinder changing process is reduce from 1752 seconds or 29.2 minutes to 877 seconds or 14.6 minute or reduce 50%, furthermore man power alaso reduce from 3 person to 1 person.
Sistem Monitoring SPM Berbasis IoT untuk Meningkatkan Produktivitas & Kualitas pada Perusahaan Manufaktur
Authors: Widya Adi Rusdi , Putra Fibi Eko , Firmansyah Andri , Fatchan Muhammad
Published: January 2024

Perancangan sistem monitoring stroke per minute (SPMMS) mesin pada sebuah organisasi perusahaan dibutuhkan untuk meningkatkan kemampuan perusahaan dalam persaingan bisnis di era Revolusi Industri 4.0, penggunaan IoT (Internet of thing), Human Machine Interface (HMI) memudahkan dalam pengembangan sistem monitoring machine salah satunya adalah untuk melakukan sistem monitoring kerja mesin saat proses produksi. Monitoring SPM dilakukan untuk meningkatkan produktivitas dan kualitas suatu mesin dalam memenuhi jadwal produksi suatu organisasi manufaktur. Perancangan pembuatan sistem monitoring (SPMMS) adalah sistem monitoring mesin secara terprogram dengan menggunakan sistem monitoring SPM secara digital pada mesin proses produksi. SPM monitoring system dilakukan untuk memberikan informasi secara real time dan online status mesin saat mesin beroperasi dan memberikan informasi produk secara kuantitas dan kualitas secara tepat dan akurat sehingga target produksi terjaga dengan baik memenuhi target yang sudah ditetapkan oleh perusahaan.
Penerapan Metode Overall Equipment Effectiveness (OEE) dan Six Big Losses untuk Mengukur Efektivitas Mesin Packing pada PT. Surya Tsabat Mandiri
Authors: Wahyudi Rizqi , Ferdana Rafi Gredita , Nugraha Andhyka Tyaz
Published: January 2024

This research aims to investigate the risk of six big losses and the effectiveness of packing machines at PT Surya Tsabat Mandiri in South Lampung using monthly data on production of bread products from March 2021 to February 2022 used in the research. Research was carried out on packing machine objects using quantitative data or data that can be measured systematically. The Overall Equipment Effectiveness (OEE) method is applied to measure the total performance of equipment as a whole according to what must be done and to assess the dominant factors of the Six Big Losses which are dominantly used by the fishbone diagram approach. The research results show that the average OEE value of packing machines is 78.17% and is still below world standards (≥85%). Apart from that, evidence was found that the most dominant six big losses factors were idling and minor stoppage losses with a value of 16.85% and reduced speed losses with a value of 7.85%. Based on the fishbone diagram, the aspects that cause speed losses on packing machines are humans (lack of operator knowledge, lack of workers guarding bread baskets), machines (repair of damaged machines, unstable center and end seal temperatures), environment (unstable production room temperature) and method (non-periodic machine maintenance). The recommendation given to reduce these two losses is to use the TPM pillars, namely education and training, autonomous maintenance and planned maintenance.
Peningkatan Kualitas Produk Abon Bandeng Babon Senyum dengan Metode Kano dan QFD
Authors: Komarudin Muhammad Echang , Negoro Yanuar Pandu , Hidayat Hidayat
Published: January 2024

In the era of rapid development of the culinary industry, companies of all sizes must compete to improve the quality of their products in order to remain relevant in the current era of free trade, Quality product design refers to the design of products that not only fulfill their basic functions, but also in accordance with quality, capacity, and appearance that satisfies customers. The company is aware of the importance of improving as well as developing the best products. Therefore, how a business wants to identify the needs and wants of consumers. This study applies QFD and Kano methods. Data collection methods in this study are conservation, interviews and distributing questionnaires. To understand the characteristics of shredded milkfish products, smile baboons that are in accordance with consumer desires, using Kano and continued with QFD analysis and then included in the House of quality, aimed at prioritizing development. The purpose of this study is to bring out what are the attributes of baboon shredded milkfish products that are expected to lift product quality. The results of this study show that there are 20 attributes that are expected by consumers and 14 technical responses, the main things of development include making new packaging, sorting and inspecting raw materials, choosing writing or fonts that are easy to read by buyers or enthusiasts, also the most important is to promote through Instagram to boost the popularity of products from smile baboons.
ПРОБЛЕМА ФОРМУВАННЯ ПРОФЕСІЙНИХ КОМПЕТЕНТНОСТЕЙ МАГІСТІВ ХІМІЇ У ВИЩИХ ЗАКЛАДАХ ОСВІТИ
Authors: Пантелеймонова Тетяна
Published: January 2024

Анотація. У статті розглядаються питання формування професійних компетентностей магістрів хімії у вищій освіті України в умовах трансформації освітнього процесу. Сучасні реалії ситуації в Україні, нові економічні виклики, соціальні вимоги до вітчизняної освітньої практики спрямовують на переосмислення зробленого та посилення компетентнісного підходу у процесі підготовки майбутніх фахівців. Автором здійснено аналіз і визначено умови процесу формування професійних компетентностей магістрів хімії у вищій освіті України в умовах трансформації освітнього процесу. Окреслено принципи впровадження компетентнісного підходу в хімічній вищій освіті. Проаналізовано освітньо-наукову програму галузі знань 10 Природничі науки, спеціальності 102 «Хімія» Харківського національного університету імені В.Н. Каразіна щодо здійснення підготовки майбутніх фахівців хімії другого (магістерського) рівня вищої освіти. Уточнено вимоги до організації процесу навчання у дистанційному та змішаному форматах. Педагогічна цінність проведеного дослідження полягає у визначенні умов організації процесу формування професійних компетентностей магістрів хімії у вищій освіті України в умовах трансформації освітнього процесу: формування ціннісно-особистісного ставлення майбутнього фахівця хімії до оволодіння професійними компетентностями, до сфери діяльності, а також розуміння свідомої відповідальності за свої дії. Обґрунтовано, що практичне значення має врахування означених принципів у впровадженні компетентнісного підходу в хімічній вищій освіті (відображення повноцінних й актуальних компетентностей за ступенем; надання здобувачам дієвої педагогічної підтримки в освітньому процесі; забезпечення ефективності освітніх ресурсів; відкритість процесу трансформації компетентностей в навчальні курси, освітні навчальні цілі та оцінювання отриманих результатів навчання здобувачів; безпечність і надійність процедури оцінювання навчальних досягнень здобувачів). Наголошено на важливості підвищення інформаційно-цифрової грамотності усіх учасників освітнього процесу, зокрема науково-педагогічних працівників, які реалізують вищу хімічну освіту; урахування вимог до ефективної організації освітнього процесу в дистанційній та змішаній формі, оцінювання рівня освітніх втрат здобувачів вищої хімічної освіти.
Ключові слова: освітній процес; здобувачі вищої освіти; магістри хімії; компетентнісний підхід; освітні втрати.