bluuebunny/crossref_metadata_2025 huggingface dataset:
The Identification of a Novel Nucleomodulin MbovP467 of Mycoplasmopsis bovis and Its Potential Contribution in Pathogenesis
Authors: Raheem Abdul , Lu Doukun , Khalid Abdul Karim , Zhao Gang , Fu Yingjie , Chen Yingyu , Chen Xi , Hu Changmin , Chen Jianguo , Chen Huanchun , Guo Aizhen
Published: March 2024

Mycoplasmopsis bovis is a causative agent of crucial diseases in both dairy and beef cattle leading to substantial economic losses. However, limited control measures for M. bovis-related diseases exist due to a lack of understanding about the virulence factors of this pathogen, a common challenge in mycoplasma research. Consequently, this study aimed to characterize a novel nucleomodulin as a virulence-related factor of M. bovis. Employing bioinformatic tools, we initially predicted MbovP467 to be a secreted protein with a nuclear localization signal based on SignalP scores and the cNLS (Nuclear Localization Signal) Mapper, respectively. Subsequently, the MbovP467 gene was synthesized and cloned into a pEGFP plasmid with EGFP labeling to obtain a recombinant plasmid (rpEGFP-MbovP467) and then was also cloned in pET-30a with a consideration for an Escherichia coli codon bias and expressed and purified for the production of polyclonal antibodies against the recombinant MbovP467 protein. Confocal microscopy and a Western blotting assay confirmed the nuclear location of MbovP467 in bovine macrophages (BoMacs). RNA-seq data revealed 220 up-regulated and 20 down-regulated genes in the rpEGFP-MbovP467-treated BoMac group compared to the control group (pEGFP). A GO- and KEGG-enrichment analysis identified associations with inflammatory responses, G protein-coupled receptor signaling pathways, nuclear receptor activity, sequence-specific DNA binding, the regulation of cell proliferation, IL-8, apoptotic processes, cell growth and death, the TNF signaling pathway, the NF-κB signaling pathway, pathways in cancer, and protein families of signaling and cellular processes among the differentially expressed up-regulated mRNAs. Further experiments, investigating cell viability and the inflammatory response, demonstrated that MbovP467 reduces BoMac cell viability and induces the mRNA expression of IL-1β, IL-6, IL-8, TNF-α, and apoptosis in BoMac cells. Further, MbovP467 increased the promoter activity of TNF-α. In conclusion, this study identified a new nucleomodulin, MbovP467, for M. bovis, which might have an important role in M. bovis pathogenesis.
Extracellular Vesicles of Patients on Peritoneal Dialysis Inhibit the TGF-β- and PDGF-B-Mediated Fibrotic Processes
Authors: Szebeni Beáta , Veres-Székely Apor , Pap Domonkos , Bokrossy Péter , Varga Zoltán , Gaál Anikó , Mihály Judith , Pállinger Éva , Takács István M. , Pajtók Csenge , Bernáth Mária , Reusz György S. , Szabó Attila J. , Vannay Ádám
Published: March 2024

Among patients on peritoneal dialysis (PD), 50–80% will develop peritoneal fibrosis, and 0.5–4.4% will develop life-threatening encapsulating peritoneal sclerosis (EPS). Here, we investigated the role of extracellular vesicles (EVs) on the TGF-β- and PDGF-B-driven processes of peritoneal fibrosis. EVs were isolated from the peritoneal dialysis effluent (PDE) of children receiving continuous ambulatory PD. The impact of PDE-EVs on the epithelial–mesenchymal transition (EMT) and collagen production of the peritoneal mesothelial cells and fibroblasts were investigated in vitro and in vivo in the chlorhexidine digluconate (CG)-induced mice model of peritoneal fibrosis. PDE-EVs showed spherical morphology in the 100 nm size range, and their spectral features, CD63, and annexin positivity were characteristic of EVs. PDE-EVs penetrated into the peritoneal mesothelial cells and fibroblasts and reduced their PDE- or PDGF-B-induced proliferation. Furthermore, PDE-EVs inhibited the PDE- or TGF-β-induced EMT and collagen production of the investigated cell types. PDE-EVs contributed to the mesothelial layer integrity and decreased the submesothelial thickening of CG-treated mice. We demonstrated that PDE-EVs significantly inhibit the PDGF-B- or TGF-β-induced fibrotic processes in vitro and in vivo, suggesting that EVs may contribute to new therapeutic strategies to treat peritoneal fibrosis and other fibroproliferative diseases.
Human Glial Cells as Innovative Targets for the Therapy of Central Nervous System Pathologies
Authors: Magni Giulia , Riboldi Benedetta , Ceruti Stefania
Published: March 2024

In vitro and preclinical in vivo research in the last 35 years has clearly highlighted the crucial physiopathological role of glial cells, namely astrocytes/microglia/oligodendrocytes and satellite glial cells/Schwann cells in the central and peripheral nervous system, respectively. Several possible pharmacological targets to various neurodegenerative disorders and painful conditions have therefore been successfully identified, including receptors and enzymes, and mediators of neuroinflammation. However, the translation of these promising data to a clinical setting is often hampered by both technical and biological difficulties, making it necessary to perform experiments on human cells and models of the various diseases. In this review we will, therefore, summarize the most relevant data on the contribution of glial cells to human pathologies and on their possible pharmacological modulation based on data obtained in post-mortem tissues and in iPSC-derived human brain cells and organoids. The possibility of an in vivo visualization of glia reaction to neuroinflammation in patients will be also discussed.
Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B-Lymphoma Cells through Tunnelling Nanotubes
Authors: Halász Henriett , Tárnai Viktória , Matkó János , Nyitrai Miklós , Szabó-Meleg Edina
Published: March 2024

Membrane nanotubes (NTs) are dynamic communication channels connecting spatially separated cells even over long distances and promoting the transport of different cellular cargos. NTs are also involved in the intercellular spread of different pathogens and the deterioration of some neurological disorders. Transport processes via NTs may be controlled by cytoskeletal elements. NTs are frequently observed membrane projections in numerous mammalian cell lines, including various immune cells, but their functional significance in the ‘antibody factory’ B cells is poorly elucidated. Here, we report that as active channels, NTs of B-lymphoma cells can mediate bidirectional mitochondrial transport, promoted by the cooperation of two different cytoskeletal motor proteins, kinesin along microtubules and myosin VI along actin, and bidirectional transport processes are also supported by the heterogeneous arrangement of the main cytoskeletal filament systems of the NTs. We revealed that despite NTs and axons being different cell extensions, the mitochondrial transport they mediate may exhibit significant similarities. Furthermore, we found that microtubules may improve the stability and lifespan of B-lymphoma-cell NTs, while F-actin strengthens NTs by providing a structural framework for them. Our results may contribute to a better understanding of the regulation of the major cells of humoral immune response to infections.
Galectin-3 Plays a Role in Neuroinflammation in the Visual Pathway in Experimental Optic Neuritis
Authors: Funaki Masako , Nio-Kobayashi Junko , Suzuki Ryoji , Bando Yoshio
Published: March 2024

Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS) featuring numerous neuropathologies, including optic neuritis (ON) in some patients. However, the molecular mechanisms of ON remain unknown. Galectins, β-galactoside-binding lectins, are involved in various pathophysiological processes. We previously showed that galectin-3 (gal-3) is associated with the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model of MS. In the current study, we investigated the expression of gal-3 in the visual pathway in EAE mice to clarify its role in the pathogenesis of ON. Immunohistochemical analysis revealed upregulation of gal-3 in the visual pathway of the EAE mice during the peak stage of the disease, compared with naïve and EAE mice during the chronic stage. Gal-3 was detected mainly in microglia/macrophages and astrocytes in the visual pathway in EAE mice. In addition, gal-3+/Iba-1+ cells, identified as phagocytic by immunostaining for cathepsin D, accumulated in demyelinating lesions in the visual pathway during the peak disease stage of EAE. Moreover, NLRP3 expression was detected in most gal-3+/Iba-1+ cells. These results strongly suggest that gal-3 regulates NLRP3 signaling in microglia/macrophages and neuroinflammatory demyelination in ON. In astrocytes, gal-3 was expressed from the peak to the chronic disease stages. Taken together, our findings suggest a critical role of gal-3 in the pathogenesis of ON. Thus, gal-3 in glial cells may serve as a potential therapeutic target for ON.
Combined Minimally Invasive Treatment of Pyeloureteral Junction Obstruction and Primary Obstructive Megaureter in Children: Case Report and Literature Review
Authors: Di Fabrizio Donatella , Tavolario Irene , Rossi Lorenzo , Nino Fabiano , Bindi Edoardo , Cobellis Giovanni
Published: March 2024

Introduction: In children, the association of ipsilateral pyeloureteral junction obstruction (PUJO) and ureterovesical junction obstruction (UVJO) is a rare malformation with a non-standardized treatment. We report a case of PUJO and UVJO treated by a combined minimally invasive surgical treatment to resolve the double urinary obstruction. The current literature was also reviewed. Case report and review: A two-month-old boy, without antenatal and postnatal signs of urinary tract anomalies, was hospitalized presenting right hydronephrosis, perirenal fluid effusion, and ascites. An acute pelvic rupture was suspected, and a retrograde pyelogram was performed, showing a primary obstructive megaureter (POM) associated with a corkscrew pyeloureteral junction. The impossibility to place a double J catheter through the pyeloureteral junction led us to achieve percutaneous nephrostomy and an abdominal drain placement. Three months later, the patient underwent a combined high-pressure balloon ureterovesical junction dilation and retroperitoneoscopic Anderson Hynes one-trocar-assisted pyeloplasty (OTAP). The literature search identified 110 children experiencing double urinary tract obstruction. All authors agreed on the difficulty to diagnose both obstructions preoperatively, but there is still no consensus on which obstruction should be relieved earlier, because the alteration in urinary vascularity during a double surgery could damage the ureter. Conclusions: The simultaneous occurrence of UPJO and UVJO is rare, with a challenging diagnosis. Prompt identification and timely surgical intervention are crucial to mitigate the risk of renal function loss attributable to obstruction and infection. Drawing from our expertise and the analysis of the existing literature, we propose employing a simultaneous double minimally invasive strategy in order to optimize the preservation of ureteral vascularity. This approach entails performing a minimally invasive pyeloplasty for the PUJ and utilizing high-pressure balloon dilatation for the UVJ.
Cyberbullying and Non-Suicidal Self-Injury (NSSI) in Adolescence: Exploring Moderators and Mediators through a Systematic Review
Authors: Predescu Elena , Calugar Iulia , Sipos Roxana
Published: March 2024

(1) Objective: This systematic review explores the intricate relationship between cyberbullying and non-suicidal self-injury (NSSI) in adolescents, acknowledging the dynamic nature of these phenomena in the evolving landscape of technology and social norms. (2) Methods: PubMed/MEDLINE, Web of Science, and EMBASE were searched, and 14 studies were selected based on the eligibility criteria, focusing on participants aged 10 to 19, cyberbullying roles, and NSSI as the predictor and outcome variables, respectively. (3) Results: Internalizing symptoms, specifically depression and anxiety, emerged as the most prominent mediators. However, factors such as externalizing symptoms, stress, and negative emotional responses (emotion reactivity, negative emotions) were also identified to play a significant role in the relationship between cyberbullying and NSSI. On the other hand, protective factors against the negative impact of cyberbullying on NSSI risk, such as strong peer connections and school engagement, were identified. (4) Discussions: This review underscores the multidimensional nature of the cyberbullying–NSSI association, emphasizing the roles of potential risk factors such as internalizing and externalizing symptoms, stress, and negative emotional response. Internalizing symptoms played a central role as pathways between cyberbullying victimization and NSSI. Additionally, social factors, including peer connections and school engagement, were found to act as protective elements. (4) Conclusion: Continuous investigation is crucial in order to adapt interventions to the evolving technological and social landscape. The study advocates for targeted interventions that prioritize positive social connections to mitigate the impact of cyberbullying on adolescent well-being.
Assessing the Implementation of the Baby-Friendly Hospital Initiative in Hungary: A Cross-Sectional Study
Authors: Hulman Anita , Varga Katalin , Csákvári Tímea , Pakai Annamária
Published: March 2024

We assessed the prevalence of the “Ten Steps to Successful Breastfeeding” in Hungary and identified possible associations of the steps with breastfeeding. Our quantitative, cross-sectional research was conducted anonymously online in Hungary with a self-administered questionnaire in 2021. Targeted sampling was used, with biological motherhood and having at least one child no older than 60 months as inclusion criteria (n = 2008). The implementation of the “Ten Steps to Successful Breastfeeding” was analyzed separately for breastfeeding and non-breastfeeding mothers. A breastfeeding mother was defined as breastfeeding for at least six months. Descriptive statistics, χ2 test, and t-test were calculated with SPSSv25 (p < 0.05). No significant differences were found between breastfeeding and non-breastfeeding mothers in terms of supplementary feeding at the advice of a health professional (p = 0.624) and in terms of assistance with breastfeeding or suggested breastfeeding positions during hospitalization (p = 0.413). Significant differences were found for receiving breastfeeding-friendly recommendations by staff (p = 0.006), valuing breastfeeding (p < 0.001), skin-to-skin contact within 1 h (p = 0.002), receiving supplementary feeding (p < 0.001), rooming-in (p < 0.001), responsive feeding, recognizing hunger signs (p < 0.001), pacifier/bottle use (p < 0.001), and availability of breastfeeding support (p = 0.005). Significant differences were observed between breastfeeding and non-breastfeeding subsamples regarding the implementation of baby-friendly steps (p < 0.001). Breastfeeding mothers experienced the implementation of more baby-friendly steps and a higher rate of breastfeeding, while there was no significant difference in the duration of exclusive (p = 0.795) and partial breastfeeding (p = 0.250) based on the results. We concluded that exposure to the Baby-Friendly Hospital Initiative may be associated with increased 6-month breastfeeding but may not influence longer durations.
Identification and Evaluation of Non-Accidental Trauma in the Pediatric Population: A Clinical Review
Authors: Schermerhorn Sophia M. V. , Muensterer Oliver J. , Ignacio Romeo C.
Published: March 2024

Non-accidental trauma (NAT) is a major cause of morbidity and mortality for children around the world and most significantly impacts children under one year of age. Prompt and comprehensive treatment of these children relies on a high index of suspicion from any medical provider that treats pediatric patients. This review discusses those most at risk for experiencing NAT, and common initial presentations, to assist providers in the identification of potential victims. In addition, this review provides guidance on the recommended workup for these patients so that the full extent of associated injuries may be identified and the appropriate healthcare team may be assembled.
Analysis of the Relationship between Psychiatric and Addiction-Related Disorders in Patients of an Outpatient Addiction Treatment Clinic for Children and Adolescents
Authors: Jandac Tomas , Stastna Lenka
Published: March 2024

Introduction: Dual diagnosis is used in addiction medicine to refer to the co-occurrence of an addiction-related disorder and another psychiatric disorder in the same individual. Adolescence is a key period for the development of both mental disorders and addictions. Objective: The aim of this study is to describe the relationships between psychiatric and addiction-related disorders in patients of the Outpatient Addiction Treatment Clinic for Children and Adolescents at the 1st Faculty of Medicine, Charles University in Prague in 2015–2022. Methods: Data were retrospectively analyzed from the hospital’s medical system, which collects basic diagnostic data on patients. Descriptive statistics and cluster analysis were performed to identify relationships between psychiatric and addiction-related disorders. Results: Of the 450 patients, 153 patients (34%) met the criteria for dual diagnosis. The most common addiction-related disorders were mental and behavioural disorders due to the use of cannabinoids (35%) and internet gaming disorder (35%). The most common psychiatric diagnoses were behavioural and emotional disorders with usual onset in childhood and adolescence (64%), with a lower prevalence in girls than in boys. Conclusions: These findings may be important for the diagnosis and treatment of risky behaviours and addictions in children and adolescents.
Spatiotemporal Assessment of Surface Solar Dimming in India: Impacts of Multi-Level Clouds and Atmospheric Aerosols
Authors: Jadhav Ashwin Vijay , Rahul P. R. C. , Kumar Vinay , Dumka Umesh Chandra , Bhawar Rohini L.
Published: March 2024

Surface solar radiation (SSR) is a fundamental energy source for an equitable and sustainable future. Meteorology-induced variability increases uncertainty in SSR, thereby limiting its reliability due to its intermittent nature. This variability depends on several meteorological factors, including clouds, atmospheric gases, and aerosol concentrations. This research investigates the detailed impact of different levels of clouds and aerosols on SSR across India. Utilizing satellite data with reanalysis retrievals, the research covers a span of three decades (30 years), from 1993 to 2022. Aerosols contributed to an average attenuation of ~13.33% on SSR, while high, mid, and low cloud conditions showed much stronger impacts, with an attenuation of ~30.80%, ~40.10%, and ~44.30%, respectively. This study reveals an alarming pattern of increasing cloud impact (Cimpact) on SSR in the recent decade, with a significant increasing rate of ~0.22% year−1 for high cloud (HCimpact) and ~0.13% year−1 for mid cloud (MCimpact) impact, while low cloud impact (LCimpact) showed minimal change. The trend of aerosol impact (Aimpact) also showed an average increase of ~0.14% year−1 across all regions. The findings underscore the imperative of considering climatic variables while studying the growing solar dimming. Our findings also will assist policymakers and planners in better evaluating the solar energy resources across India.
The “Restoration of the Restoration”: Investigation of a Complex Surface and Interface Pattern in the Roman Wall Paintings of Volsinii Novi (Bolsena, Central Italy)
Authors: Pelosi Claudia , Di Stasio Filomena , Lanteri Luca , Zuena Martina , Sardara Marta , Sodo Armida
Published: March 2024

(1) Background: The present paper reports the results of the analyses performed on the wall paintings of Room E of the Domus delle Pitture (Room of the Paintings) in the Roman archaeological site of Volsinii Novi (Bolsena, Viterbo, Central Italy) aimed at supporting the recently concluded restoration. (2) Methods: ultraviolet fluorescence photography was conducted to evaluate the conservation state. Painting materials were investigated via portable X-ray fluorescence spectrometer (p-XRF), µ-Raman spectroscopy, cross-section analysis under polarizing microscope, scanning electron microscopy coupled with energy dispersive spectroscopy (SEM-EDS), and Fourier transform infrared (FTIR) spectroscopy. (3) Results: This analysis revealed the presence of traditional pigments used in Roman wall paintings, such as ochres, earths, and Egyptian blue. Additionally, it identified pigments from previous restoration containing titanium and chromium, along with other materials present in the surface and interface of the wall painting containing arsenic, copper, lead, and vanadium, where a bluish alteration was observed. In these samples, µ-Raman spectroscopy detected calcium oxalates but also copper arsenates, likely conichalcite and/or duftite. (4) Conclusions: This result is highly relevant from an analytical point of view and in relation to the restoration decisions. Addressing the irregular bluish stains on the wall paintings poses challenges in understanding, requiring a decision on whether to remove or retain them.
Influence of Scanning Strategy and Post-Treatment on Cracks and Mechanical Properties of Selective-Laser-Melted K438 Superalloy
Authors: Zhang Bin , Yan Hua , Xia Zhisheng , Zhang Peilei , Shi Haichuan , Lu Qinghua
Published: March 2024

The feasibility of manufacturing high-performance components with complex structures is limited due to cracks in some superalloys fabricated by selective laser melting (SLM). By controlling the main process parameters such as scanning strategy, the adverse effects of cracks can be effectively reduced. In this paper, the effects of two different SLM scanning strategies with island and ‘back-and-forth’ and post-heat treatment on the cracks and mechanical properties of selective-laser-melted (SLMed) K438 alloy were investigated. The results show that the SLM method of the ‘back-and-forth’ scanning strategy had better lap and interlayer rotation angles and a more uniform distribution of laser energy compared with the island scanning strategy. The residual stress accumulation was reduced and crack formation was inhibited under this scanning strategy owing to the cooling and shrinkage process. In addition, the dislocation motion was hindered by the formation of uniformly dispersed MC carbides and γ’ phases during the SLM K438 alloy process, which resulted in the density of the as-built SLMed K438 alloy being up to 99.34%, the hardness up to 9.6 Gpa, and the tensile strength up to 1309 MPa. After post-heat treatment, the fine secondary γ’ phases were precipitated and dispersed uniformly in the Ni matrix, which effectively improved the Young’s modulus and tensile strength of the alloy by dispersing the stress-concentrated area.
Experimental Study on Tribological Properties of Coated Surface and Textured Surface under Seawater Lubrication Conditions
Authors: Liang Yingna , Zhang Zhepeng , Liu Xiaohui , Shen Miaomiao , Wang Wei , Xing Hao , Wang Cunyuan , Gao Dianrong
Published: March 2024

In order to improve the tribological properties of the flow distribution pair of the seawater axial piston pump, SAF2507 super duplex stainless steel and carbon-fiber-reinforced polyetheretherketone (CF/PEEK) were selected as the matching materials. The surface coating treatment and surface texture treatment were carried out on the flow distribution pair, and the friction and wear test were carried out by MMD-5A. In order to fully understand the friction and wear properties of the surface coating flow distribution pair and the surface texture flow distribution pair, and analyze the friction and wear mechanism, the data of the friction coefficient, wear loss, and wear surface morphology were obtained in the experiment. The results show that the coating surface has little difference in the test, the texture surface significantly reduces the friction coefficient, and the wear scar density is significantly reduced. Adding texture to the surface of the lower sample is better than adding it to the surface of the upper sample. The research results are helpful in improving the tribological performance of the flow distribution pair of the seawater axial piston pump.
Study on High-Temperature, Ultra-Low Wear Behaviors of Ti6Al4V Alloy with Thermal Oxidation Treatment
Authors: Zeng Qunfeng , Sun Shichuan , Pang Zeming , Wei Xunkai
Published: March 2024

Thermal oxidation (TO) is a simple and economical way to enhance the wear resistance of the Ti6Al4V alloy. The TO temperature has a very important effect on the tribological properties of the TiO2 layer formed. However, the impact of the oxidation temperature on the high-temperature tribological behavior of a TO-treated Ti6Al4V alloy is not clear. Therefore, the Ti6Al4V alloy was oxidized at 400 °C, 600 °C, and 700 °C for 36 h, and the sliding friction experiments were conducted at room temperature (RT) and 400 °C with a Si3N4 ball as the counter body to comparatively study the effect of the oxidation temperature on the high-temperature friction behavior of the TO-treated Ti6Al4V alloy. The results show that the TO treatment can effectively improve the wear resistance of the samples at both room and high temperatures. Among them, the oxidation-treated samples at 700 °C show the best wear resistance, with a reduction of 92.6% at high temperatures; the amount of wear loss at room temperature was so small that it was almost incalculable. At room temperature, the friction surface formed uneven agglomerate formations, resulting in an elevated coefficient of friction (CoF) compared to the untreated samples. At a high temperature, however, the CoF is reduced compared to the untreated samples due to the formation of a homogeneous transfer film in the wear area that is caused by the interaction of Si3N4 and oxygen.
Preparation of Wood-Based Carbon Quantum Dots and Promotion of Light Capture Applications
Authors: Fu Yujia , Xu Hui , Guo Qiang , Yang Dongbo , Pan Yanfei , Xue Zhenhua
Published: March 2024

CQDs are a type of fluorescent nanocarbon material that possess excellent optical properties. They have a wide range of raw material sources, making them a versatile option for various applications. The use of fluorescent materials to enhance the solar energy capture capacity of chloroplasts has the potential to significantly improve natural photosynthesis. CQDs and N-CQDs were prepared from natural Salix wood powder using a simple, green, and environmentally friendly hydrothermal method. These materials can effectively capture ultraviolet (UV) light and were used for photosynthesis to enable chloroplasts to utilize UV light that cannot be absorbed by them. The chlorophyll content of leaves treated with CQDs and N-CQDs increased, with the N-CQDs 25 mg/L treated group showing a 35.6% increase compared to the untreated group. Additionally, the treatment of CQDs and N-CQDs positively affected the transfer of electrons from photosystem II, further enhancing photosynthetic activity. This study presents ideas for expanding the use of solar energy, optimizing the photosynthesis charge transfer pathway, and improving solar energy conversion efficiency.
A Grinding Method with an Innovative, Efficient, and Weight-Saving Design for Double Helical Gears
Authors: Zhao Zemin , Shao Wen , Tang Jinyuan , Zhang Hongyan , Zhao Xingfu , Sun Hao , Dong Chao , Wang Shancheng
Published: March 2024

Grinding technology exerts an enormous effect on the surface quality of double helical gears, which are subject to stringent requirements in the aviation industry. This study presents a novel gear-gap-space-borrowing grinding method that diverges from existing techniques. Unlike conventional approaches, this method mitigates the design–manufacturing conflict by exploiting the opposing gear gap space. By borrowing space, the grinding wheel’s size can be enlarged to enhance machinability, diminish the width of the undercut, and align with the lightweight design imperative of double helical gears. The relationship between the grinding wheel diameter and the design parameters of double helical gears is analytically established, leading to the formulation of design rules. These rules facilitate the rapid selection of appropriate grinding wheel sizes for the space-borrowing method. Additionally, through geometric modeling and experimental validation, the optimal gear undercut width and the maximum wheel diameter are determined. The validity and accuracy of the proposed method are verified by simulations and experimental investigations. The study quantitatively evaluates the benefits of this method, including the expansion of the grinding wheel size, the reduction in double helical gear weight, and enhancements in processing efficiency and quality, providing a comprehensive comparison and discussion of these improvements.
Synergistic Humidification and Chemical Agglomeration to Improve Capturing the Fine Particulate Matter by Electrostatic Precipitator
Authors: Chen Hongrui , Li Hengtian , Wang Shuting , Han Yingying , Zhai Xiaoyu , Xiao Lichun
Published: March 2024

The wet electrostatic precipitator (WESP) overcomes the shortcomings of traditional electrostatic precipitators, such as dust re-entrainment and back corona. It can effectively remove high-specific-resistivity dust, with a good removal effect on PM2.5. It is proposed to adopt chemical agglomeration and humidification agglomeration technology in the wet electrostatic precipitators to achieve ultra-low dust emissions from coal-fired power plants. The results show that the addition of chemical agglomerates, surfactants, and water vapor all affect the dust diameter of coal-fired power plants. After adding sesbania gum (SG), the D50 of dust particles increases from 28.29 μm to 48.22 μm. And the D50 of dust particles is 36.46 μm when spraying 3.6 kg/h water vapor only. With the cooperation of chemical agglomeration agents and water vapor, the dust agglomeration effect and removal efficiency can be further improved. When 10 mg/L SG is synergistically combined with 2.9 kg/h water vapor, the D50 is 64.75 μm, and the dust removal efficiency reaches 97.88%. On this basis, by adding 5 mg/L of Hexadecyltrimethylammonium bromide (CTAB), the D50 is 83.06 μm, and the dust removal efficiency increases to 98.62%. The synergistic effect of chemical agglomeration and humidification agglomeration promotes the aggregation of dust from coal-fired power plants. It can improve the removal efficiency of WESP for fine particulate matter but has little impact on the operation of existing equipment. The synergistic effects of multiple agglomeration technologies are also the direction for future research on the removal efficiency of fine particulate matter.
Chitosan Gel Hydroxypropyl Methylcellulose Membranes: A Novel Approach for the Remediation of Cadmium in Aqueous Solutions and Soils
Authors: Cai Guanyu , Sun Jing , Kang Fei , Lv Qilin , Liu Jin , Wang Jie , Gao Zideng , Ren Xueqin
Published: March 2024

Cadmium (Cd2+) pollution in soil and water bodies is a significant environmental concern, necessitating effective remediation strategies. Traditional methods often fall short in efficiency, cost-effectiveness, and environmental sustainability. This study develops and evaluates the effectiveness of chitosan–gelatin–hydroxypropyl methylcellulose (CS-GEL-HPMC) membranes for Cd2+ removal from polluted environments. CS-GEL-HPMC membranes were synthesized with varying HPMC concentrations. Their structural and morphological characteristics were analyzed using UV–visible absorption spectroscopy and FT-IR. The membranes’ stability across different pH levels and their morphological traits were assessed. The adsorption efficiency for Cd2+ ions was evaluated in both aqueous solutions and soil environments under varying conditions of pH, initial ion concentration, and contact time. The CS-GEL-HPMC membranes demonstrated significant structural integrity and stability, especially at higher HPMC concentrations. UV–visible and FT-IR analyses confirmed the successful integration of HPMC into the CS-GEL matrix. In aqueous solutions, the membranes exhibited efficient Cd2+ adsorption, with the best performance observed for the CS30-GEL30-HPMC40 membrane. The adsorption capacity was influenced by contact time, initial Cd2+ concentration, and pH. In soil treatments, the membranes effectively reduced Cd2+ concentrations, with higher membrane dosages yielding better results. The incorporation of additives like (hydroxyapatite) HAP, fly ash (FA), and cement further enhanced the remediation efficiency. In summary, CS-GEL-HPMC membranes, particularly when combined with additives, emerge as a promising, sustainable solution for Cd2+ remediation in both soil and water bodies. This study highlights the potential of biopolymer-based composites in environmental clean-up efforts, offering a novel approach that is both effective and eco-friendly.
Effect of Heat Treatment on the Electrochemical and Tribological Properties of Aluminum-Bronze Coatings Deposited Used the Thermal Spraying Process
Authors: Morales Jose Alfredo , Piamba Oscar , Olaya Jhon , Vallejo Fabio
Published: March 2024

The corrosion and wear resistance of aluminum-bronze coatings deposited by thermal flame spraying were investigated after a 10 h heat treatment at 500 °C in a nitrogen atmosphere. The coatings were characterized by SEM, EDS, XRD, and XRF. Corrosion resistance was evaluated by Tafel and EIS tests, while wear resistance was assessed using a ball-on-disc test. Results showed the heat treatment compacted the coating microstructure, increased oxide content, and improved splat bonding through diffusion mechanisms. This led to enhanced corrosion resistance, evidenced by reduced corrosion current density in electrochemical tests, resulting from densification impeding electrolyte penetration. Heat treatment also increased wear resistance, as indicated by lower wear rates in ball-on-disc tests, attributable to increased hardness, reduced friction coefficients, and annealing-induced microstructural changes. Overall, the heat treatment optimizes the anticorrosive and tribological properties of thermally sprayed aluminum-bronze coatings via favorable alterations in composition, morphology, and physical characteristics. The research provides a new understanding of how thermal spray parameters and post-deposition heat treatment can be utilized to enhance the corrosion and wear resistance of aluminum-bronze coatings for marine applications.
Impact of Sterilization on the Adhesion Properties of a Polyamide 11 Coating on Textured Metal Substrates
Authors: Badji Célia , Allal Ahmed , Dupin Jean-Charles , Léonardi Frédéric
Published: March 2024

Polyamide materials are widely used for medical device coating. However, despite the fragile area at the interface, these devices must conserve their physical and mechanical performance after the sterilization process. In this work, the impact of steam sterilization, widely used in the medical sector, on the adhesion properties of biocompatible and biosourced polyamide-11-coated copper substrates was assessed. The adhesion strength, a quantitative indicator of the coating performance, was assessed thanks to a laboratory-made bench test. The surface of metal substrates was microstructured with laser engraving to enhance the coating adhesion. The Ra roughness value was varied to verify if the depth of valleys induced with the femtosecond laser could favor the interfacial anchoring. Scanning electron microscopy analyses highlighted the physical surface evolution of the polymer analyzed at the interface, across the various texturing parameter values, Ra. Fourier transform infrared spectroscopy was used to monitor the bands specific to polyamide thermo-oxidative degradation. Elemental composition deduced from X-ray photoelectron spectroscopy carried out on the coating after mechanical debonding clearly revealed that the chemical stability of the copper substrates was affected rather than the polyamide coating. Also, we discussed the impact of the chosen sterilization parameters (steam and pressure) on the formation of copper-based species detected with this technique.
COVID-19 Image Classification: A Comparative Performance Analysis of Hand-Crafted vs. Deep Features
Authors: Alinsaif Sadiq
Published: March 2024

This study investigates techniques for medical image classification, specifically focusing on COVID-19 scans obtained through computer tomography (CT). Firstly, handcrafted methods based on feature engineering are explored due to their suitability for training traditional machine learning (TML) classifiers (e.g., Support Vector Machine (SVM)) when faced with limited medical image datasets. In this context, I comprehensively evaluate and compare 27 descriptor sets. More recently, deep learning (DL) models have successfully analyzed and classified natural and medical images. However, the scarcity of well-annotated medical images, particularly those related to COVID-19, presents challenges for training DL models from scratch. Consequently, I leverage deep features extracted from 12 pre-trained DL models for classification tasks. This work presents a comprehensive comparative analysis between TML and DL approaches in COVID-19 image classification.
Application of Machine Learning to Predict Blockage in Multiphase Flow
Authors: Saparbayeva Nazerke , Balakin Boris V. , Struchalin Pavel G. , Rahman Talal , Alyaev Sergey
Published: March 2024

This study presents a machine learning-based approach to predict blockage in multiphase flow with cohesive particles. The aim is to predict blockage based on parameters like Reynolds and capillary numbers using a random forest classifier trained on experimental and simulation data. Experimental observations come from a lab-scale flow loop with ice slurry in the decane. The plugging simulation is based on coupled Computational Fluid Dynamics with Discrete Element Method (CFD-DEM). The resulting classifier demonstrated high accuracy, validated by precision, recall, and F1-score metrics, providing precise blockage prediction under specific flow conditions. Additionally, sensitivity analyses highlighted the model’s adaptability to cohesion variations. Equipped with the trained classifier, we generated a detailed machine-learning-based flow map and compared it with earlier literature, simulations, and experimental data results. This graphical representation clarifies the blockage boundaries under given conditions. The methodology’s success demonstrates the potential for advanced predictive modelling in diverse flow systems, contributing to improved blockage prediction and prevention.
Advancements in Regenerative Medicine for Aesthetic Dermatology: A Comprehensive Review and Future Trends
Authors: Trovato Federica , Ceccarelli Stefano , Michelini Simone , Vespasiani Giordano , Guida Stefania , Galadari Hassan Ibrahim , Nisticò Steven Paul , Colonna Laura , Pellacani Giovanni
Published: March 2024

The growing interest in maintaining a youthful appearance has encouraged an accelerated development of innovative, minimally invasive aesthetic treatments for facial rejuvenation and regeneration. The close correlation between tissue repair, regeneration, and aging has paved the way for the application of regenerative medicine principles in cosmetic dermatology. The theoretical substrates of regenerative medicine applications in dermo-aesthetics are plentiful. However, regenerative dermatology is an emerging field and needs more data and in vivo trials to reach a consensus on the standardization of methods. In this review, we summarize the principles of regenerative medicine and techniques as they apply to cosmetic dermatology, suggesting unexplored fields and future directions.
Enhanced Strength–Ductility Synergy of Mg-Al-Sn-Ca Alloy via Composite Asymmetric Extrusion
Authors: Chen Xiwen , Li Yuxuan , Li Zhigang , Wang Chao , Deng Hai
Published: March 2024

Fine-grain and weak-texture magnesium alloys are the long-term development targets of lightweight structural materials. In this study, a new composite asymmetric extrusion (CAE) is developed, which, coupling with an asymmetric die and an asymmetric billet, is proposed to improve the strength–ductility of the Mg-3.8Al-1.1Sn-0.4Ca alloy. The influence of the asymmetric billet on the microstructure and mechanical properties was investigated. The findings revealed that the asymmetric billet can induce greater plastic deformation, resulting in an increase in the cumulative strain and an improved nucleation rate. The CAE sheets exhibit fine grains (4.4 μm) and a weak tilted texture (7.57 mrd). Furthermore, the asymmetric billet results in the microstructure not forming a gradient microstructure under gradient strain along the transverse direction (TD) direction. The CAE sheets exhibited good mechanical properties, with a yield strength (YS) of 253 MPa, ultimate tensile strength (UTS) of 331 MPa, and elongation (EL) of 20%. This development shows promise in achieving high-efficiency, low-cost production of magnesium alloys.
Preference in the Type of Halogen Bonding Interactions within Co-Crystals of Anthraquinone with a Pair of Isosteric Perhalobenzenes
Authors: Bosch Eric , Unruh Daniel K. , Brooks Richard K. , Krueger Herman R. , Groeneman Ryan H.
Published: March 2024

The preference in the type of halogen bond accepted by anthraquinone (C14H8O2) from two isosteric donors, namely 1,4-diiodoperfluorobenzene (C6I2F4) and 1,4-diiodoperchlorobenzene (C6I2Cl4), is reported. The two co-crystals, (C6I2F4)·(C14H8O2) and (C6I2Cl4)·(C14H8O2), are sustained primarily by I···O rather than π-type halogen bonds to form these multicomponent solids. The ability for each component to engage in two divergent halogen-bonding interactions generates a one-dimensional chain structure for each co-crystal. The bias in the halogen-bonding type is due to the difference in electrostatic potential between the carbonyl oxygen and the aromatic surface on the anthraquinone. To support this observed preference, the binding energies of the I···O halogen bond were quantified for both co-crystals by using density functional theory calculations and then compared to the interaction energy for related π-type halogen bond from previously reported structures.
Chemical Composition, Crystal Structure, and Microstructure of Slags on the Korean Peninsula from the First Copper Production Remains of the 9th Century
Authors: Choi Hyoryeong , Yu Yeongchun , Cho Namchul
Published: March 2024

The scarcity of excavated early-stage smelting sites related to copper production presents significant challenges in gaining a comprehensive understanding of the copper production process. However, the archaeological site discovered in 2018 in Daeryang-ri, Jinan-gun, Jeollabuk-do, boasts a substantial number of copper smelting remains and related slags, marking it as the first copper manufacturing production site identified on the Korean Peninsula. Consequently, this study selected 10 slag samples, chosen based on surface color and characteristics indicative of a connection to copper smelting, for scientific analysis to accurately ascertain the site’s nature. The primary component analysis of the slags indicated that CuO content ranged from 0.30 to 3.29 wt%, which, although not high, reveals significant quantities of FeO and SiO2. X-ray diffraction analysis revealed the presence of minerals such as cristobalite, along with fayalite and wüstite, commonly found in slags, varying by sample. Furthermore, microstructural observation revealed circular copper particles containing sulfur and iron, indicating the presence of copper particles in a matte state that have not been refined. This analysis suggests that the slags recovered from Jinan Daeryang-ri bear evidence of iron smelting at the site, with the slag being produced as an intermediate by-product during copper production.
Influence of Spatter on Porosity, Microstructure, and Corrosion of Additively Manufactured Stainless Steel Printed Using Different Island Size
Authors: Vukkum Venkata Bhuvaneswari , Sanborn Taylor , Shepherd John , Saptarshi Sourabh , Basu Rakesh , Horn Timothy , Gupta Rajeev Kumar
Published: March 2024

Specimens of 316 L stainless steel were printed using laser powder bed fusion (LPBF), a popular metal additive manufacturing (AM) technique, with varying island sizes. Not many researchers have considered the impact of spatter while optimizing LPBF printing parameters. In this research, the influence of spatter was considered while also investigating the effect of varied island size on the microstructure, surface roughness, microhardness, and corrosion resistance of LPBF-316 L. No correlation was observed between surface roughness or microhardness and minor variations in island size. However, a correlation was drawn between varied island sizes and porosity in LPBF-316 L. The specimens associated with larger island sizes showed significantly enhanced corrosion resistance due to fewer manufacturing defects and reduced porosity, attributed to the minimal influence of the spatter. Based on analysis, the LPBF parameters were revised, which lead to superior corrosion resistance of LPBF-316 L, attributed to high density and reduced porosity.
Crystal Plasticity Modeling to Capture Microstructural Variations in Cold-Sprayed Materials
Authors: Williams Aulora , Paudel YubRaj , Mujahid Shiraz , Pepi Marc , Czech Peter , El Kadiri Haitham , Rhee Hongjoo
Published: March 2024

The high-velocity impact of powder particles in cold-spray additively manufactured (CSAM) parts creates intersplat boundaries with regions of high dislocation densities and sub-grain structures. Upon microstructure and mechanical characterization, CSAM Aluminum 6061 showed non-uniformity with spatial variation in the microstructure and mechanical properties, affecting the overall response of the additively manufactured parts. Post-processing treatments are conducted in as-printed samples to improve particle bonding, relieve residual stresses, and improve mechanical properties. In this work, we attempt to implement the effects of grain size and distribution of smaller grains along the intersplat boundaries using the grain size distribution function and powder size information to accurately predict the deformation response of cold-sprayed material using a mean-field viscoplastic self-consistent (VPSC) model. The incorporation of an intersplat boundary term in the VPSC model resulted in a stress–strain response closely matching the experimental findings, preventing the superficially high stresses observed due to Hall–Petch effects from ultra-fine-grain structures. Likewise, the results from the grain analysis showed the combined effects of grain size, orientation, and intersplat mechanisms that captured the stresses experienced and strain accommodated by individual grains.