bluuebunny/crossref_metadata_2025 huggingface dataset:
Type-I interferon pathway and DNA damage accumulation in peripheral blood of patients with psoriatic arthritis
Authors: Fragoulis George E. , Ntouros Panagiotis A. , Nezos Adrianos , Vlachogiannis Nikolaos I. , McInnes Iain B. , Tektonidou Maria G. , Skarlis Charalampos , Souliotis Vassilis L. , Mavragani Clio P. , Sfikakis Petros P.
Published: December 2023

Objectives

The abnormal DNA damage response is associated with upregulation of the type-1 interferon (IFN-I) pathway in certain rheumatic diseases. We investigated whether such aberrant mechanisms operate in psoriatic arthritis (PsA).

Methods

DNA damage levels were measured by alkaline comet assay in peripheral blood mononuclear cells from 52 PsA patients and age-sex-matched healthy individuals. RNA expression of IFIT1, MX1 and IFI44, which are selectively induced by IFN-I, was quantitated by real-time polymerase chain reaction and their composite normalized expression resulted in IFN-I score calculation. RNA expression of IL1β, IL6, TNF, IL17A and IL23A was also assessed in PsA and control subgroups.

Results

In PsA, DNA damage accumulation was increased by almost two-fold compared to healthy individuals (olive tail moment arbitrary units, mean ± SD; 9.42 ± 2.71 vs 4.88 ± 1.98, p<0.0001). DNA damage levels significantly correlated with serum C-Reactive-protein and IL6 RNA expression in PBMCs. Despite increased DNA damage, the IFN-I score was strikingly lower in PsA patients compared to controls (-0.49 ± 6.99 vs 4.24 ± 4.26; p<0.0001). No correlation was found between IFN-I pathway downregulation and DNA damage. However, the IFN-I score in a PsA subgroup was lower in those patients with higher IL1β expression, as well as in those with higher TNF/IL23A PBMCs expression.

Conclusion

DNA damage in PsA correlates with measures of inflammation but is not associated with the IFN-I pathway induction. The unexpected IFN-I downregulation, albeit reminiscent to findings in experimental models of spondyloarthritis, may be implicated in PsA pathogenesis and explained by operation of other cytokines.
Immunogenicity of PE18, PE31, and PPE26 proteins from Mycobacterium tuberculosis in humans and mice
Authors: García-Bengoa María , Vergara Emil Joseph , Tran Andy C. , Bossi Lorenzo , Cooper Andrea M. , Pearl John E. , Mussá Tufária , von Köckritz-Blickwede Maren , Singh Mahavir , Reljic Rajko
Published: December 2023

Introduction

The large family of PE and PPE proteins accounts for as much as 10% of the genome of Mycobacterium tuberculosis. In this study, we explored the immunogenicity of three proteins from this family, PE18, PE31, and PPE26, in humans and mice.

Methods

The investigation involved analyzing the immunoreactivity of the selected proteins using sera from TB patients, IGRA-positive household contacts, and IGRA-negative BCG vaccinated healthy donors from the TB endemic country Mozambique. Antigen-recall responses were examined in PBMC from these groups, including the evaluation of cellular responses in healthy unexposed individuals. Moreover, systemic priming and intranasal boosting with each protein, combined with the Quil-A adjuvant, were conducted in mice.

Results

We found that all three proteins are immunoreactive with sera from TB patients, IGRA-positive household contacts, and IGRA-negative BCG vaccinated healthy controls. Likewise, antigen-recall responses were induced in PBMC from all groups, and the proteins stimulated proliferation of peripheral blood mononuclear cells from healthy unexposed individuals. In mice, all three antigens induced IgG antibody responses in sera and predominantly IgG, rather than IgA, responses in bronchoalveolar lavage. Additionally, CD4+ and CD8+ effector memory T cell responses were observed in the spleen, with PE18 demonstrating the ability to induce tissue-resident memory T cells in the lungs.

Discussion

Having demonstrated immunogenicity in both humans and mice, the protective capacity of these antigens was evaluated by challenging immunized mice with low-dose aerosol of Mycobacterium tuberculosis H37Rv. The in vitro Mycobacterial Growth Inhibition Assay (MGIA) and assessment of viable bacteria in the lung did not demonstrate any ability of the vaccination protocol to reduce bacterial growth. We therefore concluded that these three specific PE/PPE proteins, while immunogenic in both humans and mice, were unable to confer protective immunity under these conditions.
Fluid mud induced by periodic tidal advection and fine-grained sediment settling in the Yangtze estuary
Authors: Wu Hao , Tang Jieping , Li Weihua , Xing Fei , Yang Haifei , Zhang Fan , Wang Ya Ping
Published: December 2023

This study presents in-situ observations of fluid mud in the Yangtze Estuary via a bottom tripod. Six occurrences of periodic fluid muds (F1-F6) were observed at high slack water, with suspended sediment concentration (SSC) exceeding 10 kg/m3. The thickness of the fluid mud varied across the six occurrences, reaching a maximum of 0.32m. Notably, temperature and salinity anomalies were observed within the fluid mud. The formation of fluid mud was found to be influenced by turbulence, with turbulence kinetic energy (TKE) below 5×10-4 m2/s2 favoring settling as the primary cause of formation. The critical shear stress (τcw) for fluid mud formation was estimated to be approximately 0.09 Pa. The formation of fluid muds during early tidal cycles was attributed to liquefaction and bed erosion resulting from strong waves. However, in most cases, the wave energy was insufficient for erosion, suggesting that advection played a role in sediment supply during the subsequent tide cycles. The increased cross-channel current velocity during flood facilitated the lateral transport of a significant amount of eroded sediment from shallow water into the channel. Due to its short duration, the fluid mud layer was entrained by the current before consolidation. Enhanced turbulence led to the breakdown of fluid mud at the early ebb. The critical τcw for fluid mud breakdown depended on the density and duration of the fluid mud, with a maximum value of up to 0.70 Pa. The absence of the fluid mud layer during low slack water and neap tide was associated with a reduction in advection and tidal pumping, disrupting the original sediment balance between supply and demand. These data provide valuable insights into the formation and breakdown of fluid mud, contributing to estuarine hydrodynamic modeling studies and enhancing the understanding of estuary dynamics.
Catalyzing progress in the blue economy through joint marine microbiome research across the Atlantic
Authors: Ortmann Alice C. , Goodwin Kelly D. , Bolhuis Henk , Groben René , Makhalanyane Thulani , Bowman Jeff , Robinson Shawn , Thompson Fabiano
Published: December 2023

International agreements recognize the importance of cooperative scientific research to conserve and promote sustainable development of a shared Atlantic Ocean. In 2022, the All-Atlantic Ocean Research and Innovation Alliance Declaration was signed. The All-Atlantic Declaration continues and extends relationships forged by the Galway Statement on Atlantic Ocean Cooperation and the Belém Statement on Atlantic Ocean Research and Innovation Cooperation. These efforts are consistent with programs, actions, and aims of the United Nations Decade of Ocean Science for Sustainable Development. In preparation for implementation of the All-Atlantic Declaration, members of the Marine Microbiome Working Group and the Marine Biotechnology Initiative for the Atlantic under the Galway and Belém Statements respectively, joined forces to call for cooperation across the Atlantic to increase marine microbiome and biotechnology research to promote ocean health and a sustainable bioeconomy. This article reviews the goals of the marine microbiome and biotechnology initiatives under the Galway and Belém Statements and outlines an approach to implement those goals under the All-Atlantic Declaration through a Blue Biotech and Marine Microbiome (BBAMM) collaboration.
Case report: One case of umbilical vein thrombosis in the second trimester with associated portal vein thrombosis after childbirth
Authors: Dai Wei-Wei , Hou Qi-Shu , Yang Lian-Hua , Chen Shang-Qin , Ye Ji-Feng
Published: December 2023

Background

Umbilical vein thrombosis is a rare pregnancy complication, that is difficult to detect prenatally but can lead to poor fetal outcomes.

Case presentation

We described a 33-year-old primiparae who was identified as having umbilical vein thrombosis by ultrasound at 21 weeks gestation, and the neonate was found to have a portal vein thrombus after delivery. Following enoxaparin anticoagulant therapy, the thrombus disappeared within 4 weeks. No thrombus formation occured during the 10-month follow-up, and the baby was in excellent clinical condition.

Conclusion

Owing to the poor fetal outcomes related to umbilical thrombosis, pay attention to abnormal clinical signs during prenatal ultrasound, fetal heart monitoring and counting fetal movements can help in the early identification of umbilical cord thrombosis.The findings highlight the importance of regular prenatal ultrasound evaluation, enabling early detection and monitoring of any anomalies or vascular abnormalities related to the fetal umbilical vein. Further research is warranted to explore the clinical implications and long-term outcomes associated with these findings.
Unveiling patenting strategies of therapeutics and vaccines: evergreening in the context of COVID-19 pandemic
Authors: Bacigalupo María Lorena , Pignataro María Florencia , Scopel Carolinne Thays , Kondratyuk Sergiy , Mellouk Othoman , Chaves Gabriela Costa
Published: December 2023

In the pharmaceutical sector, evergreening is considered a range of practices applied to extend monopoly protection on existing products. Filing several patent applications related to the same active pharmaceutical ingredient (API) is one of the most common manifestations of evergreening. During the COVID-19 pandemic, several health technologies were developed. This study aimed to analyze the extension of evergreening for selected health technologies for SARS-CoV-2 through patent filing strategies. Starting with the selection of three antivirals, one biological and two vaccines, a patent landscape was built based on public and private databases. Regarding these selected technologies, we analyzed some of the evergreening strategies used by different applicants, academic institutions or pharmaceutical companies and found a total of 29 applications (10 after the pandemic) for antivirals, 3 applications for a biological drug (1 after the pandemic), and 41 applications for vaccines (23 after the pandemic). Despite differences among the technologies, a common aspect found in all analyzed cases is the intense patent filing after the pandemic, aligned to the fact that those technologies were moving through the R&D process up to regulatory approval. The evergreening approach pursued has already been found in other diseases, with the risk of monopoly extension and also bringing legal uncertainty due to the lack of transparency of newer patent applications covering specific medical indications. Therefore, efforts to address evergreening should be pursued by countries, including the adoption of a public health approach to the patent examination of those technologies to prevent the granting of undeserved patents.
Challenges in characterization of transcriptomes of extracellular vesicles and non-vesicular extracellular RNA carriers
Authors: Makarova Julia , Maltseva Diana , Tonevitsky Alexander
Published: December 2023

Since its original discovery over a decade ago, extracellular RNA (exRNA) has been found in all biological fluids. Furthermore, extracellular microRNA has been shown to be involved in communication between various cell types. Importantly, the exRNA is protected from RNases degradation by certain carriers including membrane vesicles and non-vesicular protein nanoparticles. Each type of carrier has its unique exRNA profile, which may vary depending on cell type and physiological conditions. To clarify putative mechanisms of intercellular communication mediated by exRNA, the RNA profile of each carrier has to be characterized. While current methods of biofluids fractionation are continuously improving, they fail to completely separate exRNA carriers. Likewise, most popular library preparation approaches for RNA sequencing do not allow obtaining exhaustive and unbiased data on exRNA transcriptome. In this mini review we discuss ongoing progress in the field of exRNA, with the focus on exRNA carriers, analyze the key methodological challenges and provide recommendations on how the latter could be overcome.
Systematic review and meta-analysis of the efficacy and safety of electroacupuncture for poststroke dysphagia
Authors: Li Xuezheng , Lu Lijun , Fu Xuefeng , Li Hao , Yang Wen , Guo Hua , Guo Kaifeng , Huang Zhen
Published: December 2023

Introduction

Optimal treatment strategies for post-stroke dysphagia (PSD) remain to be explored. Electroacupuncture (EA) has attracted widespread attention due to its simplicity, cheapness, and safety. However, the efficacy of EA in the treatment of PSD lacks high-level evidence-based medical support. This study aimed to systematically evaluate the clinical value of EA in the treatment of PSD.

Methods

A total of seven databases were searched for relevant literature. All randomized controlled trials (RCTs) on EA alone or EA combined with other interventions for the treatment of PSD were assessed using the modified Jadad scale. The studies with a score of ≥4 were included. The quality of the included studies was then assessed using the Cochrane Collaboration’s tool. The meta-analysis was performed using Rev. Man 5.3 software.

Results

Twelve studies involving 1,358 patients were included in the meta-analysis. Meta-analysis results showed that the EA group was superior to the control group in terms of clinical response rate (OR = 2.63, 95% CI = 1.97 to 3.53) and videofluoroscopic swallowing study (VFSS) score (MD = 0.73, 95% CI = 0.29 to 1.16). There was no significant difference between the two groups in the standardized swallowing assessment (SSA) score (MD = -3.11, 95% CI = -6.45 to 0.23), Rosenbek penetration-aspiration scale (PAS) score (MD = -0.68, 95% CI = -2.78 to 1.41), Swallowing Quality of Life (SWAL-QOL) score (MD = 13.24, 95% CI = -7.74 to 34.21), or incidence of adverse events (OR = 1.58, 95% CI = 0.73 to 3.38).

Conclusion

This study shows that EA combined with conventional treatment or other interventions can significantly improve the clinical response rate and VFSS score in patients with PSD without increasing adverse reactions.

Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=396840.
Prospective evaluation of NGS-based sequencing in epilepsy patients: results of seven NASGE-associated diagnostic laboratories
Authors: Witzel Maximilian G. W. , Gebhard Christian , Wenzel Sören , Kleier Saskia , Eichhorn Birgit , Lorenz Peter , Heyden Laura von der , Kuhn Marius , Luedeke Manuel , Döcker Miriam , Jüngling Jerome , Schulte Björn , Hörtnagel Konstanze , Glaubitz Ralf , Knippenberger Sarah , Teubert Anna , Abicht Angela , Neuhann Teresa M.
Published: December 2023

Background

Epilepsy is one of the most common and disabling neurological disorders. It is highly prevalent in children with neurodevelopmental delay and syndromic diseases. However, epilepsy can also be the only disease-determining symptom. The exact molecular diagnosis is essential to determine prognosis, comorbidity, and probability of recurrence, and to inform therapeutic decisions.

Methods and materials

Here, we describe a prospective cohort study of patients with epilepsy evaluated in seven diagnostic outpatient centers in Germany. Over a period of 2 months, 07/2022 through 08/2022, 304 patients (317 returned result) with seizure-related human phenotype ontology (HPO) were analyzed. Evaluated data included molecular results, phenotype (syndromic and non-syndromic), and sequencing methods.

Results

Single exome sequencing (SE) was applied in half of all patients, followed by panel (P) testing (36%) and trio exome sequencing (TE) (14%). Overall, a pathogenic variant (PV) (ACMG cl. 4/5) was identified in 22%; furthermore, a significant number of patients (12%) carried a reported clinically meaningful variant of unknown significance (VUS). The average diagnostic yield in patients ≤ 12 y was higher compared to patients >12 y cf. Figure 2B vs. Figure 3B. This effect was more pronounced in cases, where TE was applied in patients ≤ 12 vs. >12 y [PV (PV + VUS): patients ≤ 12 y: 35% (47%), patients > 12 y: 20% (40%)]. The highest diagnostic yield was achieved by TE in syndromic patients within the age group ≤ 12 y (ACMG classes 4/5 40%). In addition, TE vs. SE had a tendency to result in less VUS in patients ≤ 12 y [SE: 19% (22/117) VUS; TE: 17% (6/36) VUS] but not in patients >12 y [SE: 19% (8/42) VUS; TE: 20% (2/10) VUS]. Finally, diagnostic findings in patients with syndromic vs. non-syndromic symptoms revealed a significant overlap of frequent causes of monogenic epilepsies, including SCN1A, CACNA1A, and SETD1B, confirming the heterogeneity of the associated conditions.

Conclusion

In patients with seizures—regardless of the detailed phenotype—a monogenic cause can be frequently identified, often implying a possible change in therapeutic action (36.7% (37/109) of PV/VUS variants); this justifies early and broad application of genetic testing. Our data suggest that the diagnostic yield is highest in exome or trio-exome-based testing, resulting in a molecular diagnosis within 3 weeks, with profound implications for therapeutic strategies and for counseling families and patients regarding prognosis and recurrence risk.
Case report: Childhood epilepsy and borderline intellectual functioning hiding an AADC deficiency disorder associated with compound heterozygous DDC gene pathogenic variants
Authors: Cursio Ida , Siliquini Sabrina , Carducci Claudia , Bisello Giovanni , Mastrangelo Mario , Leuzzi Vincenzo , Bertoldi Mariarita , Marini Carla
Published: December 2023

Aromatic L-amino acid decarboxylase (AADC) deficiency is a rare autosomal recessive neurometabolic disorder leading to severe combined serotonin, dopamine, norepinephrine, and epinephrine deficiency. We report on a female patient with borderline functioning and sporadic clear-cut focal to bilateral seizures from age 10 years. A neuropsychological assessment highlighted a mild impairment in executive functions, affecting attention span and visual–spatial abilities. Following the diagnosis of epilepsy with a presumed genetic etiology, we applied a diagnostic approach inclusive of a next-generation sequencing (NGS) gene panel, which uncovered two variants in trans in the DOPA decarboxylase (DDC) gene underlying an AADC deficiency. This compound heterozygous genotype was associated with a mild reduction of homovanillic acid, a low level of the norepinephrine catabolite, and a significant reduction of 5-hydroxyindoleacetic acid in cerebrospinal fluid. Remarkably, 3-O-methyldopa (3-OMD) and 5-hydroxytryptophan were instead increased. During the genetically guided re-evaluation process, some mild signs of dysautonomic dysfunction (nasal congestion, abnormal sweating, hypotension and fainting, excessive sleepiness, small hands and feet, and increased levels of prolactin, tiredness, and fatigue), more typical of AADC deficiency, were evaluated with new insight. Of the two AADC variants, the R347Q has already been characterized as a loss-of-function with severe catalytic impairments, while the novel L391P variant has been predicted to have a less severe impact. Bioinformatic analyses suggest that the amino acid substitution may affect affinity for the PLP coenzyme. Thus, the genotype corresponds to a phenotype with mild and late-onset symptoms, of which seizures were the clinical sign, leading to medical attention. This case report expands the spectrum of AADC deficiency phenotypes to encompass a less-disabling clinical condition including borderline cognitive functioning, drug-responsive epilepsy, and mild autonomic dysfunction.
Alzheimer’s disease: the role of extrinsic factors in its development, an investigation of the environmental enigma
Authors: Suresh Swathi , Singh S Ankul , Rushendran Rapuru , Vellapandian Chitra , Prajapati Bhupendra
Published: December 2023

In the realm of Alzheimer’s disease, the most prevalent form of dementia, the impact of environmental factors has ignited intense curiosity due to its substantial burden on global health. Recent investigations have unveiled these environmental factors as key contributors, shedding new light on their profound influence. Notably, emerging evidence highlights the detrimental role of various environmental contaminants in the incidence and progression of Alzheimer’s disease. These contaminants encompass a broad spectrum, including air pollutants laden with ozone, neurotoxic metals like lead, aluminum, manganese, and cadmium, pesticides with their insidious effects, and the ubiquitous presence of plastics and microplastics. By meticulously delving into the intricate web connecting environmental pollutants and this devastating neurological disorder, this comprehensive chapter takes a deep dive into their involvement as significant risk factors for Alzheimer’s disease. Furthermore, it explores the underlying molecular mechanisms through which these contaminants exert their influence, aiming to unravel the complex interactions that drive the pathogenesis of the disease. Additionally, this chapter proposes potential strategies to mitigate the detrimental effects of these environmental contaminants on brain health, with the ultimate goal of restoring and preserving typical cognitive function. Through this comprehensive exploration, we aim to enhance our understanding of the multifaceted relationship between neurotoxins and Alzheimer’s disease, providing a solid foundation for developing innovative in-vivo models and advancing our knowledge of the intricate pathological processes underlying this debilitating condition.
Few-shot EEG sleep staging based on transductive prototype optimization network
Authors: Li Jingcong , Wu Chaohuang , Pan Jiahui , Wang Fei
Published: December 2023

Electroencephalography (EEG) is a commonly used technology for monitoring brain activities and diagnosing sleep disorders. Clinically, doctors need to manually stage sleep based on EEG signals, which is a time-consuming and laborious task. In this study, we propose a few-shot EEG sleep staging termed transductive prototype optimization network (TPON) method, which aims to improve the performance of EEG sleep staging. Compared with traditional deep learning methods, TPON uses a meta-learning algorithm, which generalizes the classifier to new classes that are not visible in the training set, and only have a few examples for each new class. We learn the prototypes of existing objects through meta-training, and capture the sleep features of new objects through the “learn to learn” method of meta-learning. The prototype distribution of the class is optimized and captured by using support set and unlabeled high confidence samples to increase the authenticity of the prototype. Compared with traditional prototype networks, TPON can effectively solve too few samples in few-shot learning and improve the matching degree of prototypes in prototype network. The experimental results on the public SleepEDF-2013 dataset show that the proposed algorithm outperform than most advanced algorithms in the overall performance. In addition, we experimentally demonstrate the feasibility of cross-channel recognition, which indicates that there are many similar sleep EEG features between different channels. In future research, we can further explore the common features among different channels and investigate the combination of universal features in sleep EEG. Overall, our method achieves high accuracy in sleep stage classification, demonstrating the effectiveness of this approach and its potential applications in other medical fields.
Combined exposure to multiple essential elements and cadmium at early pregnancy on gestational diabetes mellitus: a prospective cohort study
Authors: Deng Guifang , Chen Hengying , Liu Yao , Zhou Yingyu , Lin Xiaoping , Wei Yuanhuan , Sun Ruifang , Zhang Zheqing , Huang Zhenhe
Published: December 2023

Background

Minerals and trace elements were involved in the pathogenesis and progression of diabetes. However, the association of mixed exposure to essential elements and toxic elements with gestational diabetes mellitus (GDM) is poorly understood.

Objective

This study aims to examine the associations between serum calcium (Ca), iron (Fe), zinc (Zn), copper (Cu), magnesium (Mg), and cadmium (Cd) concentrations in early pregnancy and GDM risk in Chinese pregnant women.

Method

A total of 1,168 pregnant women were included in this prospective cohort study. The concentrations of serum elements were measured using the polarography method before 14 gestational weeks and an oral glucose tolerance test was conducted at 24–28 gestational weeks to diagnose GDM. Binary logistic regression analysis and restricted cubic spline were applied to evaluate the association between serum individual element and GDM. Bayesian kernel machine regression (BKMR) and weighted quantile sum (WQS) regression were used to assess the associations between mixed essential elements and Cd exposure and GDM risk.

Results

The mean concentrations of Zn (124.65 vs. 120.12 μmol/L), Fe (135.26 vs. 132.21 μmol/L) and Cu (23.33 vs. 23.03 μmol/L) in the GDM group were significantly higher than those in the control group. Single-element modeling results suggested that second and fourth-quartile maternal Zn and Fe concentration, third and fourth-quartile Cu concentration and fourth-quartile Ca concentration were associated with an increased risk of GDM compared to first-quartile values. Restricted cubic spline analysis showed U-shaped and non-linear relationships between Cd and GDM. According to the BKMR models and WQS analyses, a six-element mixture was significantly and positively associated with the risk of GDM. Additionally, Cd, Zn, and Cu contributed the most strongly to the association.

Conclusion

Serum Zn, Cu, Fe, and Ca exposure during early pregnancy showed a positive association with GDM in the individual evaluation. The multiple-evaluation showed that high levels of elements mixture, particularly Cd, Zn, and Cu, may promote the development of GDM.
The research progress on synchronous endometrial and ovarian carcinoma
Authors: Gan Wenli , Bian Ce
Published: December 2023

Synchronous endometrial and ovarian carcinoma (SEOC) is the most common combination of primary double cancer in the female reproductive system. The etiology and pathogenesis of SEOC remain unclear, and clinically, it is often misdiagnosed as metastatic cancer, affecting the formulation of treatment plans and prognosis for patients. This article provides a review of its epidemiology, pathological and clinical characteristics, risk factors, pathogenesis, diagnosis, treatment, and prognosis.
High-grade fetal adenocarcinoma of the lung misdiagnosed as male breast carcinoma: a case report and literature review
Authors: Zhuo Yuejian , Xu Yanran , Qin Rong , Guo Min , Zhang Dongdong
Published: December 2023

Background and aim

High-grade fetal adenocarcinoma of the lung (HG-FLAC) is a specific subtype of lung adenocarcinoma with a poor prognosis. A lack of understanding exists because of the rarity of this disease. This study aimed to present a case of HG-FLAC with multiple metastases misdiagnosed as male breast carcinoma at the initial diagnosis.

Case presentation

The patient visited our hospital due to a month-long cough. The chest computed tomography (CT) scan revealed a mass in the left lung and chest wall, accompanied by enlargement of mediastinal lymph nodes. The magnetic resonance imaging indicated potential metastatic lesions in the brain and adrenal glands. The patient underwent a biopsy of the lesion in the right chest wall. The pathological and immunohistochemical findings indicated a high possibility of male breast cancer. However, the clinical features did not support this diagnosis. Therefore, a CT-guided percutaneous lung biopsy was performed, and the pathological examination finally indicated HG-FLAC.

Conclusions

We presented a complex yet interesting case in which HG-FLAC was misdiagnosed as male breast cancer. Our interesting case may stimulate discussions about the methods to manage patients with HG-FLAC.
Assessment of anticancer properties of cumin seed (Cuminum cyminum) against bone cancer
Authors: Chandrasekaran Rajkuberan , Krishnan Muthukumar , Chacko Sonu , Gawade Omkar , Hasan Sheik , Joseph John , George Evelin , Ali Nemat , AlAsmari Abdullah F. , Patil Sandip , Jiang Haoli
Published: December 2023

Introduction

Early-life osteosarcoma is associated with severe morbidity and mortality, particularly affecting young children and adults. The present cancer treatment regimen is exceedingly costly, and medications like ifosfamide, doxorubicin, and cisplatin have unneeded negative effects on the body. With the introduction of hyphenated technology to create medications based on plant molecules, the application of ayurvedic medicine as a new dimension (formulation, active ingredients, and nanoparticles) in the modern period is rapidly growing. The primary source of lead compounds for the development of medications for avariety of ailments is plants and their products. Traditionally, Cuminum cyminum (cumin) has been used as medication to treat a variety of illnesses and conditions.

Methods

The cumin seed was successfully extracted with solvents Hexane, Chloroform, Methanol, Ethanol and Acetone. Following the solvent extraction, the extract residue was assayed in MG63 cells for their anti-proliferative properties.

Results

First, we used the [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] (MTT) assay to test the extracted residue’s cytotoxicity. The results show that hexane extract Half-maximal inhibitory concentration (IC50 86 µG/mL) effciently inhibits cells by causing programmed cell death. Furthermore, using the Acridine orange/ethidium bromide (AO/EB) staining method, the lactate dehydrogenase assay, and the reactive oxygen species assay using the Dichloro-dihydro-fluorescein diacetate (DCHFDA) staining method, we have demonstrated that the hexane extract causes apoptosis in MG63 cells. Furthermore, flow cytometry research revealed that the hexane extract stops the cell cycle in the S phase. In addition, the hexane extract limits colony formation and the migration potential as shown by the scratch wound healing assay. Furthermore, the extract from cumin seeds exhibits remarkable bactericidal properties against infections that are resistant to drugs. Gas chromatography analysis was used to quantitatively determine the hexane and methanolic extract based on the experimental data. The primary chemical components of the extract are revealed by the study, and these help the malignant cells heal. The present study finds that there is scientific validity in using cumin seeds as a novel method of anticancer therapy after undergoing both intrinsic and extrinsic research.
The effects of upper- vs. lower-body aerobic exercise on perceived pain in individuals with chronic knee pain: a randomised crossover trial
Authors: Deere Rachel , Chowdhury Enhad , Tabor Abby , Thompson Dylan , Bilzon James L. J.
Published: December 2023

Background and objectives

Some patients with chronic knee pain experience an increase in knee pain following a single bout of exercise involving their knee joint, which can negatively affect exercise adherence and thus result in reduced overall health and lack of disease management. We want to determine whether a single bout of upper-body (UB) aerobic arm-ergometry exercise is effective in reducing the experience of pain in those with chronic knee pain compared with lower-body (LB) aerobic leg ergometry exercise.

Methods

A total of 19 individuals (women = 11, men = 8; age = 63 ± 8 years; body mass index = 24 ± 3 kg/m2) who suffered from chronic knee pain for ≥3 months took part in this study. Arm-ergometry and cycle-ergometry exercises were performed for 30 min at a moderate intensity, separated by 7 days. Pain intensity was assessed by means of a visual analogue scale (VAS) pre- and post-exercise and for 7 days post-exercise. Pressure pain threshold (PPT) and mechanical detection threshold (MDT) were measured pre- and post-exercise at both local and distal anatomical sites. Data are presented as mean ± SD.

Results

VAS pain was significantly reduced (p = 0.035) at 1 day post-exercise following the UB exercise trial (−1.4 ± 0.8) when compared with the LB exercise trial (+0.1 ± 2.1). Both UB and LB exercises were effective in reducing local and distal PPT. MDT responses were heterogeneous, and no differences between the UB and LB exercise conditions were noted.

Conclusion

An acute bout of upper-body aerobic arm-ergometry exercise evoked a significant decrease in the affected knee joint pain in individuals with chronic knee pain of up to 24 h/1 day post-exercise compared with lower-body aerobic exercise. While the exact mechanisms remain unclear, upper-body exercise may offer a viable, novel therapeutic treatment for patients with chronic knee pain.
“I cannot see your fear!” Altered recognition of fearful facial expressions in anorexia nervosa
Authors: Vaioli Giulia , Bastoni Ilaria , Villa Valentina , Mendolicchio Leonardo , Castelnuovo Gianluca , Mauro Alessandro , Scarpina Federica
Published: December 2023

Background

The evidence about facial emotion recognition in anorexia nervosa as the role of alexithymic traits on this emotional ability is conflicting and heterogeneous.

Objective

We assessed the capability of recognizing facial expressions of two primary emotions, fear, and anger, in the context of anorexia nervosa.

Methods

Women affected by anorexia nervosa were compared with healthy weight women in a well-established implicit facial emotion recognition task. Both reaction time and level of accuracy were computed. Moreover, the individual levels of alexithymia were assessed through a standard self-report questionnaire.

Results

Participants with anorexia nervosa reported a significantly lower performance in terms of reaction time and accuracy when the emotion of fear—but not anger—was the target. Notably, such an alteration was linked to the levels of alexithymia reported in the self-report questionnaire.

Conclusion

In anorexia nervosa, difficulties in processing facial fearful (but not angry) expressions may be observed as linked to higher expressions of alexithymic traits. We suggested future research in which emotional processing will be investigated taking into account the role of the bodily dimensions of emotional awareness.
Zuranolone – synthetic neurosteroid in treatment of mental disorders: narrative review
Authors: Marecki Rafał , Kałuska Joanna , Kolanek Agata , Hakało Dominika , Waszkiewicz Napoleon
Published: December 2023

With each passing year, the number of people suffering from mental disorders grows at a disturbing speed. Neuroactive steroids are a new promising group of drugs with the potential for use in many diseases like postpartum depression, postnatal psychosis, major depression, insomnia, bipolar disorder, and Parkinson’s tremor, due to their ability to modulate the activity of GABAA receptor. Neurosteroids are progesterone metabolites that are synthesized from cholesterol or steroid hormones in various brain regions. They regulate neuronal development, regeneration, and neurotransmission. They are implicated in mood disorders, anxiety disorders, schizophrenia, PTSD, and impulsive aggression. Neurosteroids have been studied for their potential to prevent or treat neurodegenerative diseases such as Alzheimer’s disease and HIV-associated dementia. They can promote neurogenesis, neuronal survival, myelination, and memory function. They can also affect the growth and sensitivity of hormone-dependent brain tumors such as gliomas. Zuranolone, a newly registered neurosteroid drug has shown huge flexibility in both clinical and ambulatory treatment thanks to its pharmacokinetic traits, especially the possibility for oral administration, unlike its predecessor Brexanolone. Zuranolone is a synthetic positive allosteric modulator of the GABAA receptor that can be taken orally. The review aims to summarize the current knowledge on zuranolone as a novel neurosteroid drug for various mental disorders, especially for postpartum mental disorders for which this drug was meant originally. It covers studies indexed in the PubMed, Scopus, and Web of Science databases published since 2017. Keywords used in the search, as well as inclusion and exclusion criteria, are given in the aims and methodology section. The review explains the evidence for the role of neurosteroids, especially allopregnanolone, in the pathophysiology and treatment of postpartum depression. It discusses the mechanisms of neurosteroid action, the changes in neurosteroid levels during pregnancy and postpartum, and the clinical trials of brexanolone and zuranolone, two synthetic analogs of allopregnanolone, for postpartum depression. It provides an overview of the biosynthesis and metabolism of neurosteroids in the central and peripheral nervous system. Furthermore, it explains the different sources and pathways of neurosteroid production and the factors that influence their synthesis and regulation, such as stress, hormones, drugs, and genetic variations. The review also explores the potential relevance of neurosteroids for other psychiatric disorders, such as major depression, bipolar disorder, post-traumatic stress disorder (PTSD), schizophrenia, and premenstrual dysphoric disorder. Finally, it highlights the associations between neurosteroid levels and symptom severity and the effects of neurosteroid modulation on mood, cognition, and neuroplasticity.
The influence of demographic and lifestyle factors on blood donation delay among student population: a retrospective study
Authors: Feng Wang , Yun Wang , Le Wang , Zhi-guo Xu , Hai-ying Yang , Shu-fang Wu , Zhen-yan Wei , Yi-zhu Chen , Quan Sun , Jing-xian Fei
Published: December 2023

Objective

This study analysed blood donation deferral trends, reasons and demographic/lifestyle characteristics among students in Huzhou City. The aim was to understand the health status of students and reduce the deferral rate.

Methods

Data on blood donation deferral among students in Huzhou City from 2018 to 2022 were collected and analysed. Deferral trends and main reasons were investigated. Using demographic and lifestyle data from 2,619 cases in 2022, a risk prediction model for deferral was constructed.

Results

The deferral rate among students in Huzhou City from 2018 to 2022 was 12.60% (p = 0.000, 95%CI: 12.14–13.06%), showing a significant increasing trend. Temporary deferral was the main reason, with alanine aminotransferase (ALT), blood pressure (BP) and haemoglobin (Hb) as the main deferral factors. ALT had a deferral rate of 5.23% (4.92–5.53%), BP 3.30% (3.06–3.55%), and Hb 2.92% (2.68–3.15%). Demographic and lifestyle characteristics in 2022 showed no significant differences between education level, household registration and deferral rate (p > 0.05). However, age, sex, blood donation history, sleep quality, diet and mental state had variable effects on ALT, BP, and Hb deferrals (p < 0.05). Logistic regression showed that sex, blood donation history, sleep quality, diet and mental status were independent risk factors for ALT deferral (p < 0.05), with odds ratios (ORs) of 5.057, 2.735, 1.594, 3.679, and 1.957, respectively. Age, blood donation history, sleep quality and mental state were independent risk factors for BP deferral (p < 0.05), with ORs of 0.256, 3.658, 6.042, and 1.812, respectively. Gender, blood donation history and diet were independent risk factors for Hb deferral (p < 0.05), with ORs of 0.244, 0.542, and 3.103, respectively.

Conclusion

Students’ health problems require attention. Effective health education should improve self-health management and pre-donation health behaviour to encourage regular blood donation.
RISE: an open-source architecture for interdisciplinary and reproducible human–robot interaction research
Authors: Groß André , Schütze Christian , Brandt Mara , Wrede Britta , Richter Birte
Published: December 2023

In this article, we present RISE—a Robotics Integration and Scenario-Management Extensible-Architecture—for designing human–robot dialogs and conducting Human–Robot Interaction (HRI) studies. In current HRI research, interdisciplinarity in the creation and implementation of interaction studies is becoming increasingly important. In addition, there is a lack of reproducibility of the research results. With the presented open-source architecture, we aim to address these two topics. Therefore, we discuss the advantages and disadvantages of various existing tools from different sub-fields within robotics. Requirements for an architecture can be derived from this overview of the literature, which 1) supports interdisciplinary research, 2) allows reproducibility of the research, and 3) is accessible to other researchers in the field of HRI. With our architecture, we tackle these requirements by providing a Graphical User Interface which explains the robot behavior and allows introspection into the current state of the dialog. Additionally, it offers controlling possibilities to easily conduct Wizard of Oz studies. To achieve transparency, the dialog is modeled explicitly, and the robot behavior can be configured. Furthermore, the modular architecture offers an interface for external features and sensors and is expandable to new robots and modalities.
Cable Bacteria Get Energy Through Electrical Teamwork
Authors: Geerlings Nicole M. J. , Middelburg Jack J. , Polerecky Lubos , Meysman Filip J. R.
Published: December 2023

Cable bacteria are very different from most other bacteria. First of all, they are multicellular, and each cable bacterium has tens of thousands of cells connected in a chain called a filament that can be several centimeters long. What makes cable bacteria really special is the way they get the energy they need for living: the cells within a filament have to work together to generate energy, and this involves electricity! In this article, we will describe these fascinating bacteria and tell you about recent research that shows how the cells within a filament work together.
Validity of a virtual reality endoscopic retrograde cholangiopancreatography simulator: can it distinguish experts from novices?
Authors: Georgiou Konstantinos , Boyanov Nikola , Antonakis Pantelis , Thanasas Dimitrios , Sandblom Gabriel , Enochsson Lars
Published: December 2023

Background

There is a lack of evidence regarding the effectiveness of virtual simulators as a means to acquire hands-on exposure to endoscopic retrograde cholangiopancreatography (ERCP). The present study aimed to assess the outcome and construct validity of virtual ERCP when training on the GI II Mentor simulator.

Methods

A group of seven experienced endoscopists were compared with 31 novices. After a short introduction, they were requested to carry out three virtual ERCP procedures: diagnosing and removing a common bile duct (CBD) stone; diagnosing and taking brush cytology from a hilar stenosis; and, finally, diagnosing and treating a cystic leakage with a BD stent. For each task, the total time required to complete the task, time required to correctly view the papilla, total time of irradiation, time to deep cannulation, time to define diagnosis, time to complete sphincterotomy, and time to complete the respective intervention were measured. Cannulation of the BD, correct diagnosis, sphincterotomy, and time to complete intervention were assessed by an assessor blinded to the status of the endoscopist who performed the virtual ERCP.

Results

The time required to visualize the papilla and to cannulate deeply when removing the BD stone was significantly shorter for the experts (both p < 0.05). The time to visualize the papilla, cannulate deeply, reach a diagnosis, complete sphincterotomy, and complete the intervention was significantly shorter for the experts when managing cystic leakage (all p < 0.05). In diagnosing and taking brush cytology from a hilar stenosis, there was only a trend toward the experts needing less time for the deep cannulation of the BD (p = 0.077).

Conclusion

The performance differed between experts and novices, especially in the management of cystic leakage. This corroborates the construct validity of the GI II Mentor simulator.
Effects of alkaline mineral complex supplementation on production performance, serum variables, and liver transcriptome in calves
Authors: Guo Cheng , Wang Xiaowei , Dai Dongwen , Kong Fanlin , Wang Shuo , Sun Xiaoge , Li Shengli , Xu Xiaofeng , Zhang Lili
Published: December 2023

Calf diarrhea causes huge economic losses to livestock due to its high incidence and mortality rates. Alkaline mineral complex water is an alkaline solution containing silicon, sodium, potassium, zinc, and germanium, and has biological benefits and therapeutic effects. This study aimed to evaluate the impact of alkaline mineral complex water supplementation on the health of calves and to investigate the effect of Alkaline mineral complex water supplementation on neonatal calf serum variables and the liver transcriptome. Sixty Holstein calves (age 1.88 ± 0.85 days, weight 36.63 ± 3.34 kg) were selected and randomly divided into two groups: the T group (treatment group with alkaline mineral complex water supplemented during the experiment) and C group (control group without alkaline mineral complex water supplementation). Alkaline mineral complex water supplementation significantly increased the body weight for calves aged 60 d and average daily gain during the experimental period (1–60 d). In addition, Alkaline mineral complex water supplementation could significantly decrease the diarrhea rate for calves aged 16–30 d, enhance the T-AOC, IgG, IGF-1, and IGFBP-2 in concentrations. The results of KEGG enrichment analysis in transcriptomics indicate that Alkaline mineral complex water supplementation inhibited the target IL-1B gene of the NF-kappa B signaling pathway of liver. Alkaline mineral complex water supplementation decreased calf diarrhea and improved partial immune function, anti-inflammatory activity, antioxidant capacity, and health of calves. Alkaline mineral complex is a candidate to replace medicated feed additives. Alkaline mineral complex waterAlkaline mineral complex waterAlkaline mineral complex waterAlkaline mineral complex waterAlkaline mineral complex waterAlkaline mineral complex waterAlkaline mineral complex water.