bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Dietary Amaranth (Amaranthus hybridus chlorostachys) Supplemented with Enzyme Blend on Egg Quality, Serum Biochemistry and Antioxidant Status in Laying Hens
Authors: Janmohammadi Hossein , Hosseintabar-Ghasemabad Babak , Oliyai Majid , Alijani Sadegh , Gorlov Ivan Fedorovich , Slozhenkina Marina Ivanovna , Mosolov Aleksandr Anatolievich , Suarez Ramirez Lourdes , Seidavi Alireza , Laudadio Vito , Tufarelli Vincenzo , Ragni Marco
Published: February 2023

A feeding trial was performed to assess the effects of dietary raw amaranth (Amaranthus hybridus chlorostachys) grain (RAG), with or without an enzyme blend, on the productive performance, blood biochemistry, and antioxidant status in laying hens. The trial was conducted following a completely randomized design by factorial method, including five levels of RAG (0, 10, 20, 30, and 40%, respectively) and two levels of enzyme blend (0 −E and 0.025 +E %). A total of 960 White Leghorn (Hy-line W-36) laying hens (56 weeks of age) were divided into 10 groups with eight repetitions, including 12 birds. The trial period was ten weeks. Results showed that RAG levels in feed (>10%) led to a significant decrease in blood total cholesterol (TC), but they also significantly decreased feed conversion ratio (FCR) (p ˂ 0.05) as measured by feed intake (FI), hen daily production (HDP), egg weight (EW), and mass (EM), leading to overall worse productivity compared to the control group. On the contrary, the addition of the enzyme blend led to an improvement in the investigated production traits (p ˂ 0.05), with the exception of HDP. The enzyme blend was also capable of recovering productive performance when combined with low concentrations of RAG (10%) (p ˂ 0.05), and RAG × enzyme blend groups showed the lowest values of TC (p ˂ 0.05). Moreover, the interaction effects for atherogenic index (LDL/HDL) indicated a significant and promising reduction in response to the addition of RAG both in the presence and absence of the enzyme blend (p ˂ 0.05), and this additive also significantly reduced levels of egg yolk cholesterol (p ˂ 0.05). In summary, the evidence gathered in this trial showed that dietary RAG had positive effects on egg quality characteristics, leading to the production of low-cholesterol eggs, and, at the same time, it may improve the health status of laying hens. Furthermore, the addition of an enzyme blend allowed feeding up to 10% RAG in the diet, leading to an optimal balance between animal productivity and the beneficial effects of RAG.
Antioxidative and Cytoprotective Efficacy of Ethanolic Extracted Cranberry Pomace against Salmonella Enteritidis Infection in Chicken Liver Cells
Authors: Ahmed Nada , El-Fateh Mohamed , Amer Magdy S. , El-Shafei Reham A. , Bilal Muhammad , Diarra Moussa S. , Zhao Xin
Published: February 2023

Salmonella enterica serovar Enteritidis is a globally significant zoonotic foodborne pathogen. Chicken liver is a vital organ that has been recently implicated in several reported human salmonellosis outbreaks in the U.S. One promising strategy for reducing Salmonella in chickens could be through supplementation with natural antimicrobial additives. Ethanolic extracted cranberry pomace (CPOH) is an excellent source of bioactive polyphenolic compounds with antioxidant and antimicrobial activities. However, the protective effect of CPOH against S. Enteritidis-induced chicken hepatic cell damage remains unclear. In this study, we used a chicken hepatoma cell (LMH) infection model to investigate the protective effects and potential mechanisms of CPOH. CPOH increased the viability of S. Enteritidis-infected LMH cells. Furthermore, CPOH reduced the adhesion and invasion of S. Enteritidis to LMH cells. CPOH downregulated the expression of Rho GTPase genes that are essential for Salmonella’s entry into LMH cells. Additionally, the expression of antioxidant regulatory genes, such as Nrf2, HO-1, Txn, and Gclc, was increased. Our data show that CPOH effectively protected LMH cells from cell damage through the inhibition of S. Enteritidis adhesion and invasion, as well as the induction of the expression of master antioxidant genes. These findings offer opportunities to develop sustainable, safe, and economic strategies to reduce the colonization and pathogenesis of Salmonella.
Extraction, Identification and Antioxidant Activity of 3-Deoxyanthocyanidins from Sorghum bicolor L. Moench Cultivated in China
Authors: Wu Yanbei , Wang Yali , Liu Zhengyan , Wang Jing
Published: February 2023

3-deoxyanthocyanidins (3-DAs) are typical flavonoids found in sorghum bran, and they have received much attention in recent years owing to their bioactivities. To further enhance the resource utilization of sorghums cultivated in China, three brewing sorghum cultivars (Liaoza-48, Liaonuo-11, and Liaonian-3) commonly used in China were selected as raw materials for the construction of an extraction technology system for 3-DAs and the clarification of their structures and bioactivities. Based on single-factor experiments and response surface analysis, the optimized system for the extraction of 3-DAs from sorghum grains was as follows: a hydrochloric acid-methanol solution (1:100, v/v) was the extraction solvent, the solid-liquid ratio was 1:20 (g/mL), the extraction time was 130 min, and the temperature was 40 °C. This extraction system was simple and feasible. High performance liquid chromatography analysis suggested that the main monomeric compounds of the extracted 3-DAs were Apigeninidin (AE) and Luteolinidin (LE). Among the three selected sorghum grains, Liaoza-48 had the highest amount of AE (329.64 μg/g) and LE (97.18 μg/g). Antioxidant experiments indicated that the 3-DAs extracted from Liaoza-48 showed higher free-radical scavenging activities for DPPH, ABTS+, and hydroxyl radicals than those extracted from Liaonuo-11 and Liaonian-3. These results provide basic data and technical support for the high-value and comprehensive utilization of sorghums in China.
Oxidative Stress in Depression: The Link with the Stress Response, Neuroinflammation, Serotonin, Neurogenesis and Synaptic Plasticity
Authors: Correia Ana Salomé , Cardoso Armando , Vale Nuno
Published: February 2023

Depression is a prevalent, complex, and highly debilitating disease. The full comprehension of this disease is still a global challenge. Indeed, relapse, recurrency, and therapeutic resistance are serious challenges in the fight against depression. Nevertheless, abnormal functioning of the stress response, inflammatory processes, neurotransmission, neurogenesis, and synaptic plasticity are known to underlie the pathophysiology of this mental disorder. The role of oxidative stress in disease and, particularly, in depression is widely recognized, being important for both its onset and development. Indeed, excessive generation of reactive oxygen species and lack of efficient antioxidant response trigger processes such as inflammation, neurodegeneration, and neuronal death. Keeping in mind the importance of a detailed study about cellular and molecular mechanisms that are present in depression, this review focuses on the link between oxidative stress and the stress response, neuroinflammation, serotonergic pathways, neurogenesis, and synaptic plasticity’s imbalances present in depression. The study of these mechanisms is important to lead to a new era of treatment and knowledge about this highly complex disease.
Oxidative Damages on the Alzheimer’s Related-Aβ Peptide Alters Its Ability to Assemble
Authors: Cheignon Clémence , Collin Fabrice , Sabater Laurent , Hureau Christelle
Published: February 2023

Oxidative stress that can lead to oxidation of the amyloid-β (Aβ) peptide is considered a key feature in Alzheimer’s disease (AD), influencing the ability of Aβ to assemble into β-sheet rich fibrils that are commonly found in senile plaques of AD patients. The present study aims at investigating the fallouts of Aβ oxidation on the assembly properties of the Aβ peptide. To accomplish this, we performed kinetics and analysis on an oxidized Aβ (oxAβ) peptide, resulting from the attack of reactive oxygen species (ROS) that are formed by the biologically relevant Cu/Aβ/dioxygen/ascorbate system. oxAβ was still able to assemble but displayed ill-defined and small oligomeric assemblies compared to the long and thick β-sheet rich fibrils from the non-oxidized counterpart. In addition, oxAβ does affect the assembly of the parent Aβ peptide. In a mixture of the two peptides, oxAβ has a mainly kinetic effect on the assembly of the Aβ peptide and was able to slow down the formation of Aβ fibril in a wide pH range [6.0–7.4]. However, oxAβ does not change the quantity and morphology of the Aβ fibrils formed to a significant extent. In the presence of copper or zinc di-cations, oxAβ assembled into weakly-structured aggregates rather than short, untangled Cu-Aβ fibrils and long untangled Zn-Aβ fibrils. The delaying effect of oxAβ on metal altered Aβ assembly was also observed. Hence, our results obtained here bring new insights regarding the tight interconnection between (i) ROS production leading to Aβ oxidation and (ii) Aβ assembly, in particular via the modulation of the Aβ assembly by oxAβ. It is the first time that co-assembly of oxAβ and Aβ under various environmental conditions (pH, metal ions …) are reported.
Extracellular Vesicles, as Drug-Delivery Vehicles, Improve the Biological Activities of Astaxanthin
Authors: Jang Young Jun , Cha Byung Seok , Kim Doyeon , Lee Eun Sung , Kim Seokjoon , Han Jinjoo , Shin Jiye , Kim Seokhwan , Park Ki Soo
Published: February 2023

Astaxanthin (AST) exhibits potent antioxidant and anti-inflammatory activities but poor stability and biological efficacy, which limit its application in the food and medical industries. In the present study, a new strategy was proposed to enhance the biological activities of AST using fetal bovine serum-derived extracellular vesicles (EVs). Saponin-assisted incubation was used to load AST owing to its high encapsulation efficiency and loading capacity. AST-incorporated EVs (EV-ASTs) maintained their original EV morphology and showed high stability at 4 °C, 25 °C, and 37 °C over a 28-day period, which was attributed to the protective environment provided by the phospholipid bilayer membrane of the EVs. Additionally, the EV-ASTs exhibited excellent antioxidant and anti-inflammatory activities in HaCaT keratinocytes and RAW 264.7 macrophage cells, respectively; these were significantly higher than those of free AST. Furthermore, the mechanism associated with the enhanced biological activities of EV-ASTs was evaluated by analyzing the expression of genes involved in antioxidation and anti-inflammation, in parallel with cellular in vitro assays. These results provide insights into methods for improving the performance of hydrophobic drugs using nature-derived EVs and will contribute to the development of novel drug-delivery systems.
Reversible Image Processing for Color Images with Flexible Control
Authors: Sugimoto Yuki , Imaizumi Shoko
Published: February 2023

In this paper, we propose an image processing method for color images to reversibly achieve flexible functions. Most previous research has focused on reversible contrast enhancement (CE) for grayscale images. When we directly apply these methods to color images, hue distortion is caused. Several previous methods have been proposed for color images. These methods, however, only have a CE function. We previously proposed a reversible method for color images that enhances the brightness contrast and improves the saturation. Without losing the advantages of our previous method, we propose a new method to expand the ability of image processing. The proposed method reversibly achieves not only CE and saturation improvement but also sharpening or smoothing and brightness increases or decreases. It ensures full reversibility and thus perfectly reconstructs raw images in any case. The experimental results demonstrate the effectiveness of the proposed method in terms of image quality and reversibility.
Cohort-Based Analysis of Foreign Residents’ Growth in Japan
Authors: Oo Sebal , Tsukai Makoto
Published: February 2023

In Japan, the cohort structure of foreign residents and its spatial distribution significantly change along with the acceptance of a specialized and technically skilled workforce. This study aims to analyze the transition of foreign residents’ characteristics with time series with statistical viewpoints, in order to clarify the policy building for foreign migrants by local government. A nonnegative matrix factorization model (NMF) is applied to the cohort data of foreign residents in 47 Japanese prefectures in 2010, 2015 and 2020. By applying NMF to the ratio by cohort shifting, the common basis of foreign residents among the prefectures and its structures are obtained. The results show the cohort transition for foreign migrants with infants or children were significantly different, especially between Tohoku and Kyusyu regions from 2010 to 2020. The elderly cohort also had a significant change in Tohoku region from 2015 to 2020. Since the regions highlighted in this analysis include many depopulated areas, and the capacity of those local governments for the policy building would not be enough, they should be supported well by the national government.
Seismic Performance of Fully Prefabricated L-Shaped Shear Walls with Grouted Sleeve Lapping Connectors under High Axial Compression Ratio
Authors: Yu Qiong , Tang Ziming , Zhang Xingkui , Fan Baoxiu , Zhang Zhi , Chen Zhenhai
Published: February 2023

The grouted sleeve lapping connector called the APC connector has the advantages of high fault tolerance, convenient construction, compacted grouting, and low cost, together with realizing full prefabrication of vertical components. In this paper, a quasi-static test of two fully prefabricated L-shaped walls connected with two types of APC connector and a cast-in-place wall was carried out under high axial compression ratio (0.5) to compare their seismic performance. The results indicated that the two types of connectors effectively transferred the rebar stress in the prefabricated walls, and the failure modes and final crack distribution of the prefabricated walls and the cast-in-place wall were basically identical. The failure of the cast-in-place wall occurred at the root of the wall limb, while the failure of the prefabricated walls occurred at the top of the sleeve due to the constraint of the sleeve. The bearing capacity, stiffness, ductility, and energy dissipation of the prefabricated specimen connected by the type-I sleeve was comparable to that of the cast-in-place wall, while the prefabricated wall connected by the type-II sleeve showed greater bearing capacity, stiffness, and ductility. Finally, some suggestions for seismic design of prefabricated components connected with APC connectors are proposed.
EYOLOv3: An Efficient Real-Time Detection Model for Floating Object on River
Authors: Zhang Lili , Xie Zhiqiang , Xu Mengqi , Zhang Yi , Wang Gaoxu
Published: February 2023

At present, the surveillance of river floating in China is labor-intensive, time-consuming, and may miss something, so a fast and accurate automatic detection method is necessary. The two-stage convolutional neural network models appear to have high detection accuracy, but it is hard to reach real-time detection, while on the other hand, the one-stage models are less time-consuming but have lower accuracy. In response to the above problems, we propose a one-stage object detection model EYOLOv3 to achieve real-time and high accuracy detection of floating objects in video streams. Firstly, we design a multi-scale feature extraction and fusion module to improve the feature extraction capability of the network. Secondly, a better clustering algorithm is used to analyze the size characteristics of floating objects to design the anchor box, enabling the network to detect objects more effectively. Then a focus loss function is proposed to make the network effectively overcome the sample imbalance problem, and finally, an improved NMS algorithm is proposed to solve the object suppressed problem. Experiments show that the proposed model is efficient in detection of river floating objects, and has better performance than the classical object detection method and the latest method, realizing real-time floating detection in video streams.
Robots in Inspection and Monitoring of Buildings and Infrastructure: A Systematic Review
Authors: Halder Srijeet , Afsari Kereshmeh
Published: February 2023

Regular inspection and monitoring of buildings and infrastructure, that is collectively called the built environment in this paper, is critical. The built environment includes commercial and residential buildings, roads, bridges, tunnels, and pipelines. Automation and robotics can aid in reducing errors and increasing the efficiency of inspection tasks. As a result, robotic inspection and monitoring of the built environment has become a significant research topic in recent years. This review paper presents an in-depth qualitative content analysis of 269 papers on the use of robots for the inspection and monitoring of buildings and infrastructure. The review found nine different types of robotic systems, with unmanned aerial vehicles (UAVs) being the most common, followed by unmanned ground vehicles (UGVs). The study also found five different applications of robots in inspection and monitoring, namely, maintenance inspection, construction quality inspection, construction progress monitoring, as-built modeling, and safety inspection. Common research areas investigated by researchers include autonomous navigation, knowledge extraction, motion control systems, sensing, multi-robot collaboration, safety implications, and data transmission. The findings of this study provide insight into the recent research and developments in the field of robotic inspection and monitoring of the built environment and will benefit researchers, and construction and facility managers, in developing and implementing new robotic solutions.
A 12.4–32 GHz CMOS Down-Conversion Mixer for 28 GHz 5G New Radio (NR)
Authors: Lin Yo-Sheng , Lan Kai-Siang
Published: February 2023

We report a low voltage (VDD) and power (PDC) 12.4–32 GHz CMOS down-conversion mixer with high conversion gain (CG) for 28 GHz 5G communications. A quarter-wavelength (λ/4) transmission line (TL) and a coupling capacitor (Cc), named the λ/4-TL-C-based coupler, is proposed. This is the way to attain low-VDD, independent RF transconductance (gm)-stage bias, harmonic suppression, and near perfect coupling from the RF gm stage to the LO switch transistors. The body-self-forward-bias (BSFB) technique, i.e., connection of the gm-stage transistors’ body to drain via a large body resistance, is used for threshold voltage (Vth) and VDD reduction and substrate leakage suppression. CG and noise figure (NF) enhancement at the same or even a lower PDC is achieved because lower VDD and higher gm (due to larger bias current) are used. To facilitate the RF measurement, a compact Wilkinson-power-divider-based balun with small-phase deviation and amplitude imbalance is included at RF and LO inputs. The mixer consumes 6.5 mW and achieves a CG of 14.4 ± 1.5 dB for 12.4–32 GHz (i.e., 3 dB bandwidth (f3dB) of 19.6 GHz), a lowest noise figure (NFmin) of 7 dB, and figure-of-merit (FOM) of 0.023, which is one of the best results ever reported for millimeter-wave (mm-wave) down-conversion mixers with an f3dB larger than 10 GHz and PDC lower than 10 mW.
Elastic–Plastic Stage of Transverse Deformations in the Compressed Zone of Real Reinforced Concrete Beams
Authors: Iskhakov Iakov , Ribakov Yuri , Holschemacher Klaus , Kaeseberg Stefan
Published: February 2023

The relationship between transverse and longitudinal deformations (Poisson ratio) in the compressed zone of bending reinforced concrete elements is still not properly considered in designs. This factor is important as it is related to the formation of transverse cracks in the compressed zone of the elements. The main aim of the present research was an experimental investigation of the Poisson ratio in the compressed zone of real single- and two-layer reinforced concrete beams as well as a comparison with the theoretical model proposed in this study. The elastic and elastic–plastic stages were analyzed using experimental data for real beams with a span of 8 m. It was shown that the term “Poisson coefficient” had a limited validity for elastic longitudinal deformations up to 0.5‰. After reaching this limit, the suitable term was “Poisson ratio”. The obtained results allowed a more accurate prediction of transverse deformations and the corresponding cracks, defining a new limit state for bending reinforced concrete elements. Therefore, the authors recommend that the obtained results are considered in order to increase the design accuracy of reinforced concrete bending elements. The outcomes of this study and the proposed theoretical concept should be included in modern design provisions.
Data Mining and Augmented Reality: An Application to the Fashion Industry
Authors: Fani Virginia , Antomarioni Sara , Bandinelli Romeo , Ciarapica Filippo Emanuele
Published: February 2023

The wider implementation of Industry 4.0 technologies in several sectors is increasing the amount of data regularly collected by companies. Those unstructured data need to be quickly elaborated to make on-time decisions, and the information extracted needs to be clearly visualized to speed up operations. This is strongly perceived in the quality field, where effective management of the trade-off between increasing quality controls to intercept product defects and decreasing them to reduce the delivery time represents a competitive challenge. A framework to improve data analysis and visualization in quality management is proposed, and its applicability is demonstrated with a case study in the fashion industry. A questionnaire assesses its on-field usability. The main findings refer to overcoming the lack in the literature of a decision support framework based on the joint application of association rules mining and augmented reality. The successful implementation in a real scenario has a twofold aim: on the one hand, sample sizes are strategically revised according to the supplier performance per product category and material; on the other hand, the daily quality controls are speeded up through accurate suggestions about the most occurrent defect and location per product characteristics, integrated with extra tips only for trainees.
Accuracy of Guided Endodontics in Posterior Teeth
Authors: Haarmann Benjamin , Leontiev Wadim , Magni Eva , Kühl Sebastian , Dagassan-Berndt Dorothea , Weiger Roland , Connert Thomas
Published: February 2023

The purpose of this ex vivo study was to determine the accuracy of template-based guided endodontics for access cavity preparation and root canal detection in posterior teeth. First, three maxillary and four mandibular models were constructed using 67 premolars and molars, with a total number of 135 main root canals. Cone beam computed tomography (CBCT) and three-dimensional 3D surface scans of each model were performed and matched in order to plan access cavity preparation and design templates virtually. Template-guided access cavity preparation was then performed for each tooth, followed by postoperative CBCT scanning. Deviations between planned and prepared access cavities were measured after superimposition of the pre- and postoperative CBCT scans, and they were analyzed using descriptive and multivariate statistics. All root canals (135/135) were detected utilizing guided endodontics. The mean angle deviation was 1.4 degrees, and the mean deviations at the tip and base of the bur were 0.24–0.31 mm and 0.26–0.29 mm, respectively. This study demonstrated that guided endodontics is an accurate and predictable method for endodontic access cavity preparation in posterior teeth.
Speech Communication System Based on Piezoelectric Electret Mechanical Antenna
Authors: Wang Shuopu , Yang Jing , Lu Ping , Yang Shaolong , Guo Limin , Hao Yanan , Huang Kai , Xu Jianchun
Published: February 2023

Low-frequency electromagnetic waves have strong penetration and low attenuation performance, making them widely used in long-wave communication areas such as underwater communication and earthquake prediction. Mechanical antennas are seen as a promising alternative to traditional low-frequency resonant antennas, as they offer the potential for miniaturization and low power consumption. However, existing communication systems using mechanical antennas are usually rudimentary, incompatible, and inconvenient to use. To address these problems, a novel speech communication system based on a piezoelectric electret mechanical antenna was proposed. This communication system includes a hardware system, a communication protocol for mechanical antennas, and a software system with a modular structure. This system includes hardware, a communication protocol, and a modular software system. The results of the experiments showed that this communication system provides real-time speech communication with a user-friendly design, high compatibility with different communication devices, and easy customization to meet specific requirements. This design holds great potential for applications in mineral detection, seismic research, submarine communication, industrial RF control, and other related mechanical antenna applications.
Assessment of Musculoskeletal Pain and Physical Demands Using a Wearable Smartwatch Heart Monitor among Precast Concrete Construction Workers: A Field Case Study
Authors: Arias Oscar , Groehler James , Wolff Mike , Choi Sang D.
Published: February 2023

This study aimed to quantify musculoskeletal symptoms/pain and characterize the physical demands at work and outside of work among precast concrete workers. Direct heart rate (HR) measurements and self-reported activity levels were used to estimate the physical demands. A total of 27 precast construction workers participated in a survey, and 21 wore a HR monitor smart watch for seven days. The HR data were parsed in minutes associated with occupational and nonoccupational physical activity. Correlation analysis and multivariate regression models were conducted to assess the associations between direct measured physical activity with self-reported physical activity, body mass index (BMI), years of work, smoking, and Borg ratings of perceived exertion (RPE). Approximately half of the participants experienced musculoskeletal symptoms in the last seven days and moderate functional limitations in carrying out activities of daily living (ADLs). The regression model revealed a positive relationship between direct measured moderate occupational physical activity (OPA) and the Borg RPE. Furthermore, an inverse association was found with BMI, smoking status, and years of work. The workers accrued a median of 415 min of moderate OPA per week. The findings showed a high amount of moderate OPA minutes per week and musculoskeletal issues among the precast concrete workers.
High-Pressure Effects on Selected Properties of Pea and Soy Protein Isolates
Authors: Queirós Rui , Ferreira Rita , Saraiva Jorge A. , Lopes-da-Silva José A.
Published: February 2023

The use of vegetable proteins has been the focus of research efforts to develop new products and/or to replace other sources of protein. Ergo, there is a need to assess the effects of new processing technologies on this type of protein. This work evaluated the influence of high-pressure processing (HPP) (pressure: 200, 400 and 600 MPa; holding time: 5, 10 and 15 min) on selected properties of pea (PPI) and soy (SPI) protein isolates at three pH values (6, 7 and 8). SPI presented a higher percentage of soluble proteins than PPI, still, HPP increased protein solubility of both isolates. This effect was more pronounced on SPI, particularly at pH 7 and 8, where the percentage of soluble proteins almost tripled under some HPP conditions. Similarly, the surface hydrophobicity also increased with HPP for proteins from both sources, increasing, in general, with increasing pressure and holding time. On the contrary, the content of free sulfhydryl groups generally decreased with HPP for proteins from both sources, suggesting a complex balance between protein unfolding and further aggregation under certain conditions. The effects of HPP on the emulsifying properties of the protein isolates were dependent on pH, pressure, holding time and whether the soluble or total fraction of the protein isolates were used.
Management of Used COVID-19 Personal Protective Equipment: A Bibliometric Analysis and Literature Review
Authors: de la Torre Bayo Juan Jesús , Martín-Lara María Ángeles , Calero Hoces Mónica , Sánchez Castillo Pedro Miguel , Pula Héctor J. , Zamorano Montserrat
Published: February 2023

Using a science mapping approach, we analyzed the exponential increase in the number of scientific documents about the negative environmental impacts produced by waste from personal protective equipment (PPE), especially face masks, used to reduce SARS-CoV-2 transmission worldwide. Our results revealed that India, China, and Canada are leaders in this research field, which is clearly related to environmental issues, but also the solutions developed from an engineering point of view. Our analysis of the most-relevant documents in the field uncovered the considerable negative effects of PPE waste in aquatic media, its contribution to greenhouse gas emissions, effects on wildlife, etc. To reduce the negative environmental impacts of PPE waste, we need to implement innovative ecodesign strategies for their green production, including their re-use as and the use of recycling materials, but also a collaboration with the population to reduce PPE waste at its source. Both action lines could be materialized by establishing a collective, extended producer responsibility system for PPE to ensure their sustainable production and consumption. These well-implemented strategies will contribute to maintaining progress towards achieving sustainable development goals.
Low-Cost Handheld Spectrometry for Detecting Flavescence Dorée in Vineyards
Authors: Imran Hafiz Ali , Zeggada Abdallah , Ianniello Ivan , Melgani Farid , Polverari Annalisa , Baroni Alice , Danzi Davide , Goller Rino
Published: February 2023

This study was conducted to evaluate the potential of low-cost hyperspectral sensors for the early detection of Flavescence dorée (FD) from asymptomatic samples prior to symptom development. In total, 180 leaf spectra from 60 randomly selected plants (three leaves per plant) were collected by using two portable mini-spectrometers (Hamamatsu: 340–850 nm and NIRScan: 900–1700 nm) at five vegetative growth stages in a vineyard with grape variety Garganega. High differences in the Hamamatsu spectra of the two groups were found in the VIS-NIR (visible–near infrared) spectral region while very small differences were observed in the NIRScan spectra. We analyzed the spectral data of two sensors by using all bands, features reduced by an ensemble method, and by genetic algorithms (GA) to discriminate the asymptomatic healthy (FD negative) and diseased (FD positive) leaves using five different classifiers. Overall, high classification accuracies were found in case of the Hamamatsu sensor compared to the NIRScan sensor. The feature selection techniques performed better compared to all bands, and the highest classification accuracy of 96% was achieved when GA features of the Hamamatsu sensor were used with the logistic regression (LR) classifier on test samples. A slightly low accuracy of 85% was achieved when the features (selected by the ensemble method) of the Hamamatsu sensor were used with the support vector machine (SVM) classifier by using leave-one-out (LOO) cross-validation on the whole dataset. Results demonstrated that employing a feature selection technique can provide a valid tool for determining the optimal bands that can be used to identify FD disease in the vineyard. However, further validation studies are required, as this study was conducted using a small dataset and from the single grapevine variety.
Simulation-Based Analysis of the Effect of New Immersion Freezing Equipment on the Freezing Speed
Authors: Chang Jiang , Sun Pengda , Gong Xue , Sun Zhihui , Wang Jing , Li Xiaoyan
Published: February 2023

Modeling software was used to improve the efficiency of existing immersion freezing equipment. The concept of adding a rotating hopper device was proposed to make pork rotate with the rotating hopper in a flowing refrigerant carrier to achieve the effect of shortening the freezing time. In order to make up for the shortcomings of time-consuming processes and the limited information obtained from the measurement of the central temperature of pork, this paper establishes a mathematical model of the pre-freezing process using computational fluid dynamics (CFD) software to simulate the three-dimensional unsteady state of the pork. The simulation results show that when the rotating hopper is stationary, the refrigerant circulation flow shortens the freezing speed by 490 s compared to the freezing speed in the stationary state of the refrigerant carrier, and the freezing time efficiency is improved by 9.5%. When the refrigerant carrier flow rate v = 0 m/s and when the hopper speed r = 2 rad/s, the central temperature of pork drops rapidly from 26 °C to −15 °C in 0~1250 s, the decline leveled off at 1250–2310 s, and finally dropped to −18 °C, requiring a total of 5410 s. The freezing time of the immersion and freezing equipment was reduced by 2830 s after the addition of the rotating device, improving efficiency by nearly 55%. When the rotation speed r is increased from 2 rad/s to 4 rad/s, the freezing time is shortened by 1290 s and the freezing efficiency is increased by nearly 53%. The addition of the rotating device will make the pork freezing effect more uniform.
Gene Drive: Past, Present and Future Roads to Vertebrate Biocontrol
Authors: McFarlane Gus R. , Whitelaw C. Bruce A. , Lillico Simon G.
Published: February 2023

Scientists have long sought a technology to humanely control populations of damaging invasive pests in a species-specific manner. Gene drive technology could see this become a reality. This review charts the twists and turns on the road to developing gene drives in vertebrates. We focus on rodents, as these will likely be the first targets, and trace the journey from the early understanding of selfish genetic elements to engineering gene drives in mice; before discussing future research focuses and the crucial role that public perception and governance will play in the application of this technology. The realisation of robust gene drive strategies in vertebrate pests has the potential to revolutionise biocontrol.
Artistic Responses to Crossing the Kālā Pānī
Authors: Ali Grace Aneiza
Published: February 2023

Between 1838 and 1917, a British system of indentured servitude replaced the enslavement of African peoples with Indian labor in the Americas and the Caribbean. Almost a quarter of a million indentured Indian laborers came to British Guiana and would form the foundation of the majority of the Indian population in present-day Guyana. These men and women would spend nearly eight decades toiling on sugar plantations and rice fields before the brutal system of labor was abolished. This curatorial essay explores the work of three key contemporary artists of Guyanese heritage—Maya Mackrandilal, Michael Lam, and Suchitra Mattai—who underscore St. Vincent-born poet Derek Walcott’s seminal words “the sea is history” with an exploration of the sea as a weapon of rupture. Collectively, their artworks return us to a British past to offer a visceral reminder of the perilous kālā pānī crossing [Hindi for “black waters”], marking the sea the place where ancestral histories, trauma, and survival all share space. Grounding us in the present and pointing us to a future, I illustrate how these artworks also function as contemporary tools of remembrance and repair.
Comparison of Speech, Spatial, and Qualities of Hearing Scale (SSQ) and the Abbreviated Profile of Hearing Aid Benefit (APHAB) Questionnaires in a Large Cohort of Self-Reported Normal-Hearing Adult Listeners
Authors: Srinivasan Nirmal , O’Neill Sadie
Published: February 2023

The Speech, Spatial, and Qualities of Hearing Scale (SSQ) and the Abbreviated Profile of Hearing Aid Benefit (APHAB) are two most commonly used questionnaires in the audiology clinic to assess an individual’s self-perception of their hearing ability. Here, we present the outcomes of these two questionnaires on a large group of self-reported normal hearing adult listeners. A total of 254 self-reported normal-hearing younger and older adults completed the SSQ and the APHAB questionnaire. The younger participants completed the questionnaires through Qualtrics, whereas the older participants completed the questionnaire through Qualtrics and a traditional pen-and-paper method. The younger listeners perceived a higher ability compared to the older adults in all the SSQ subscales (Speech, Spatial, and Qualities) and reported a lesser frequency of the problems in three of the four APHAB subscales (Ease of communication, Reverberation, and Background Noise). There was no significant difference in the frequency of the problems reported in the Aversiveness subscale. Self-reported normal-hearing listeners do not rate their listening ability at the top of the ability scale. Additionally, the large dataset presented here has a potential normative value for the SSQ and the APHAB questionnaires for self-reported normal-hearing adult listeners.
On the Application of the Block Hybrid Methods to Solve Linear and Non-Linear First Order Differential Equations
Authors: Shateyi Stanford
Published: February 2023

Block hybrid methods with intra-step points are considered in this study. These methods are implemented to solve linear and nonlinear single and systems of first order differential equations. The stability, convergence, and accuracy of the proposed methods are qualitatively investigated through the absolute and residual error analysis in some selected cases. A number of different numerical examples are tested to demonstrate the efficiency and applicability of the proposed methods. In this study we also implement the proposed methods to solve chaotic systems such as the Glukhvsky–Dolzhansky system, producing very comparable results to those already in the literature.
Best Approximation Results for Fuzzy-Number-Valued Continuous Functions
Authors: Font Juan J. , Macario Sergio
Published: February 2023

In this paper, we study the best approximation of a fixed fuzzy-number-valued continuous function to a subset of fuzzy-number-valued continuous functions. We also introduce a method to measure the distance between a fuzzy-number-valued continuous function and a real-valued one. Then, we prove the existence of the best approximation of a fuzzy-number-valued continuous function to the space of real-valued continuous functions by using the well-known Michael selection theorem.
Effect of Lactic Acid Fermentation on Phytochemical Content, Antioxidant Capacity, Sensory Acceptability and Microbial Safety of African Black Nightshade and African Spider Plant Vegetables
Authors: Irakoze Marie Lys , Wafula Eliud Nalianya , Owaga Eddy Elkana
Published: February 2023

Traditional preparation of African indigenous vegetables (AIVs) such as African black nightshade (Solanum nigrum) and African spiderplant (Cleome gynandra) involves either boiling and discarding the first water or lengthy boiling. Fermentation is considered a better alternative processing technique due to the enhanced retention of phytochemical contents and sensory properties. However, little is known about the impact of lactic acid fermentation on the phytochemical content, antioxidant capacity, sensory acceptability and microbial safety of the African black nightshade and African spiderplant. This study aimed to ferment AIVs using combined starter cultures (Lactobacillus fermentum and Lactococcus lactis) and further determine their effect on the phytochemical content (phenolic compounds and flavonoids), antioxidant capacity, sensory acceptability and microbial safety of the vegetables. There was a marked increase in phenol and flavonoid contents in all fermented vegetables (p < 0.05). The highest phenol content was 228.8 mg/g GAE (gallic acid equivalent) in the starter-culture-inoculated African black nightshade, while flavonoid content was 10.6 mg/g QE (quercetin equivalent) in the same. Starter-culture-inoculated AIVs presented significantly higher antioxidant capacity with a 60–80% radical scavenging activity compared to levels in uninoculated batches (p < 0.05). Fermented vegetables were more liked than the boiled vegetables and were microbiologically safe. In conclusion, lactic fermentation of AIVs increased phytochemical contents (phenolic compounds and flavonoids), maintained antioxidant capacity and improved product safety and sensory acceptability. Therefore, fermentation and consumption of the African indigenous vegetables are to be encouraged.
CO2 Pressure-Dependent Microstructure and Morphology of Carbon for Energy Storage: Unraveling the Role of CO2 in Green Synthesis of Carbon Materials
Authors: Li Peng , Chen Yun , Liang Chu , Zeng Chengfu , Zhang Xiaoyu , Zhong Haichang , Zhang Wenxian , Zheng Xiaohua , Gao Mingxia
Published: February 2023

Advanced carbon materials have played an important function in the field of energy conversion and storage. The green and low-carbon synthesis of elemental carbon with controllable morphology and microstructure is the main problem for carbon materials. Herein, we develop a green and low-carbon method to synthesize porous carbon by reacting CO2 with LiAlH4 at low temperatures. The starting reaction temperatures are as low as 142, 121, and 104 °C for LiAlH4 reacting with 1, 30, and 60 bar CO2, respectively. For the elemental carbon, the porosity of elemental carbon gradually decreased, whereas its graphitization degree increased as the CO2 pressure increased from 1 bar to 60 bar. CO2 serves as one of the two reactants and the CO2 pressure can adjust the thermodynamic and kinetic properties of the formation reaction for synthesizing elemental carbon. The mechanism for CO2 pressure-dependent microstructure and morphology of carbon is discussed on the basis of the formation reaction of elemental carbon and gas blowing effect of H2 and CO2. The elemental carbon with different morphology and microstructure exhibits distinct electrochemical lithium storage performance including reversible capacity, rate capability, cycling stability, and Coulombic efficiency, owing to their different lithium storage mechanism. The elemental carbon synthesized at 30 bar CO2 delivers the highest reversible capacity of 506 mAh g−1 after 1000 cycles even at 1.0 A g−1. Advanced energy storage technology based on the green and low-carbon synthesis of carbon materials is a requisite for providing a stable and sustainable energy supply to meet the ever-growing demand for energy.
Ιnclusion Complexes of Magnesium Phthalocyanine with Cyclodextrins as Potential Photosensitizing Agents
Authors: Kavetsou Eleni , Tsoukalas-Koulas Charalampos , Katopodi Annita , Kalospyros Alexandros , Alexandratou Eleni , Detsi Anastasia
Published: February 2023

In this work, the preparation of inclusion complexes, (ICs) using magnesium phthalocyanine (MgPc) and various cyclodextrins (β-CD, γ-CD, HP-β-CD, Me-β-CD), using the kneading method is presented. Dynamic light scattering (DLS) indicated that the particles in dispersion possessed mean size values between 564 to 748 nm. The structural characterization of the ICs by infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy provides evidence of the formation of the ICs. The release study of the MgPc from the different complexes was conducted at pH 7.4 and 37 °C, and indicated that a rapid release (“burst effect”) of ~70% of the phthalocyanine occurred in the first 20 min. The kinetic model that best describes the release profile is the Korsmeyer–Peppas. The photodynamic therapy studies against the squamous carcinoma A431 cell line indicated a potent photosensitizing activity of MgPc (33% cell viability after irradiation for 3 min with 18 mW/cm2), while the ICs also presented significant activity. Among the different ICs, the γ-CD-MgPc IC exhibited the highest photokilling capacity under the same conditions (cell viability 26%). Finally, intracellular localization studies indicated the enhanced cellular uptake of MgPc after incubation of the cells with the γ-CD-MgPc complex for 4 h compared to MgPc in its free form.
Crosstalk between Inflammation and Hemorrhage/Coagulation Disorders in Primary Blast Lung Injury
Authors: Li Junfeng , Zhang Jianfeng , Shi Mingyu , Yu Sifan , Ji Mengjun , Liang Yangfan , Meng Xiangyan
Published: February 2023

Primary blast lung injury (PBLI), caused by exposure to high-intensity pressure waves from explosions in war, terrorist attacks, industrial production, and life explosions, is associated with pulmonary parenchymal tissue injury and severe ventilation insufficiency. PBLI patients, characterized by diffused intra-alveolar destruction, including hemorrhage and inflammation, might deteriorate into acute respiratory distress syndrome (ARDS) with high mortality. However, due to the absence of guidelines about PBLI, emergency doctors and rescue teams treating PBLI patients rely on experience. The goal of this review is to summarize the mechanisms of PBLI and their cross-linkages, exploring potential diagnostic and therapeutic targets of PBLI. We summarize the pathophysiological performance and pharmacotherapy principles of PBLI. In particular, we emphasize the crosstalk between hemorrhage and inflammation, as well as coagulation, and we propose early control of hemorrhage as the main treatment of PBLI. We also summarize several available therapy methods, including some novel internal hemostatic nanoparticles to prevent the vicious circle of inflammation and coagulation disorders. We hope that this review can provide information about the mechanisms, diagnosis, and treatment of PBLI for all interested investigators.
Mechanistic Analysis of CCP1 in Generating ΔC2 α-Tubulin in Mammalian Cells and Photoreceptor Neurons
Authors: Hotta Takashi , Plemmons Alexandra , Gebbie Margo , Ziehm Trevor A. , Blasius Teresa Lynne , Johnson Craig , Verhey Kristen J. , Pearring Jillian N. , Ohi Ryoma
Published: February 2023

An important post-translational modification (PTM) of α-tubulin is the removal of amino acids from its C-terminus. Removal of the C-terminal tyrosine residue yields detyrosinated α-tubulin, and subsequent removal of the penultimate glutamate residue produces ΔC2-α-tubulin. These PTMs alter the ability of the α-tubulin C-terminal tail to interact with effector proteins and are thereby thought to change microtubule dynamics, stability, and organization. The peptidase(s) that produces ΔC2-α-tubulin in a physiological context remains unclear. Here, we take advantage of the observation that ΔC2-α-tubulin accumulates to high levels in cells lacking tubulin tyrosine ligase (TTL) to screen for cytosolic carboxypeptidases (CCPs) that generate ΔC2-α-tubulin. We identify CCP1 as the sole peptidase that produces ΔC2-α-tubulin in TTLΔ HeLa cells. Interestingly, we find that the levels of ΔC2-α-tubulin are only modestly reduced in photoreceptors of ccp1−/− mice, indicating that other peptidases act synergistically with CCP1 to produce ΔC2-α-tubulin in post-mitotic cells. Moreover, the production of ΔC2-α-tubulin appears to be under tight spatial control in the photoreceptor cilium: ΔC2-α-tubulin persists in the connecting cilium of ccp1−/− but is depleted in the distal portion of the photoreceptor. This work establishes the groundwork to pinpoint the function of ΔC2-α-tubulin in proliferating and post-mitotic mammalian cells.