bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptional profiling of long non-coding RNAs regulating fruit cracking in Punica granatum L. under bagging
Authors: Wang Yuying , Zhao Yujie , Wu Yaqiong , Zhao Xueqing , Hao Zhaoxiang , Luo Hua , Yuan Zhaohe
Published: October 2022

Fruit cracking tremendously damages the appearance of fruit, easily leads to pathogen invasion, greatly reduces the marketability and causes immense economic losses. The pivotal role of long non-coding RNAs (lncRNAs) in diverse biological processes has been confirmed, while the roles of lncRNAs underlying fruit cracking remain poorly understood. In this study, the incidence of fruit cracking was 7.26% under the bagging treatment, the control group was 38.11%, indicating that bagging considerably diminished the fruit cracking rate. LncRNA libraries for fruit cracking (FC), fruit non-cracking (FNC) and fruit non-cracking under bagging (FB) in pomegranate (Punica granatum L.) were performed and analysed via high-throughput transcriptome sequencing. A total of 3194 lncRNAs were obtained with a total length of 4898846 nt and an average length of 1533.77 nt in pomegranate. We identified 42 differentially expressed lncRNAs (DELs) and 137 differentially expressed mRNAs (DEGs) in FC vs FNC and 35 DELs and 160 DEGs in FB vs FC that formed co-expression networks respectively, suggesting that there are involved in phytohormone signaling pathway, lignin catabolic process, lipid transport/binding, cutin biosynthetic process and cell wall organization. We also found that 18 cis-acting DELs regulated 18 target genes, and 10 trans-acting DELs regulated 24 target genes in FC vs FNC, 23 DELs regulate 23 target genes for the cis-acting lncRNAs and 12 DELs regulated 36 target genes in FB vs FC, which provides an understanding for the regulation of the fruit cracking. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results demonstrated that DELs participated in calcium ion binding, glycerophospholipid metabolism, flavonoid biosynthetic process, cell wall biogenesis, xyloglucan metabolic process, hormone signal transduction and starch and sucrose metabolism. Our findings provide new insights into the roles of lncRNAs in regulating the fruit cracking and lay the foundation for further improvement of pomegranate quality.
Analysis of plant cell death-inducing proteins of the necrotrophic fungal pathogens Botrytis squamosa and Botrytis elliptica
Authors: Malvestiti Michele C. , Steentjes Maikel B. F. , Beenen Henriek G. , Boeren Sjef , van Kan Jan A. L. , Shi-Kunne Xiaoqian
Published: October 2022

Fungal plant pathogens secrete proteins that manipulate the host in order to facilitate colonization. Necrotrophs have evolved specialized proteins that actively induce plant cell death by co-opting the programmed cell death machinery of the host. Besides the broad host range pathogen Botrytis cinerea, most other species within the genus Botrytis are restricted to a single host species or a group of closely related hosts. Here, we focused on Botrytis squamosa and B. elliptica, host specific pathogens of onion (Allium cepa) and lily (Lilium spp.), respectively. Despite their occurrence on different hosts, the two fungal species are each other’s closest relatives. Therefore, we hypothesize that they share a considerable number of proteins to induce cell death on their respective hosts. In this study, we first confirmed the host-specificity of B. squamosa and B. elliptica. Then we sequenced and assembled high quality genomes. The alignment of these two genomes revealed a high level of synteny with few balanced structural chromosomal arrangements. To assess the cell death-inducing capacity of their secreted proteins, we produced culture filtrates of B. squamosa and B. elliptica that induced cell death responses upon infiltration in host leaves. Protein composition of the culture filtrate was analysed by mass spectrometry, and we identified orthologous proteins that were present in both samples. Subsequently, the expression of the corresponding genes during host infection was compared. RNAseq analysis showed that the majority of the orthogroups of the two sister species display similar expression patterns during infection of their respective host. The analysis of cell death-inducing proteins of B. squamosa and B. elliptica provides insights in the mechanisms used by these two Botrytis species to infect their respective hosts.
Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen
Authors: Jia Wenqing , Wang Yanli , Mi Zhaorong , Wang Zheng , He Songlin , Kong Dezheng
Published: October 2022

Pollen morphology, pollen vigor, and long-term pollen storage are critical for plant cross-breeding and genetic improvement of Exochorda racemosa. We developed a protocol for viability determination and storage of E. racemosa pollen for breeding new varieties. The medium components for E. racemosa pollen germination was optimized by using an Orthogonal Assay Test Strategy (OATS). The germination rates of E. racemosa pollen were investigated after storing at different temperatures and different storage periods. The size of E. racemosa pollen was medium with three germination ditches, and the sculptural type of pollen was striate. Red ink and 2,3,5-triphenyl tetrazolium chloride (TTC) can effectively distinguish viable pollen from the unviable pollen of E. racemosa. The most suitable medium (CK2) for E. racemosa was composed of 150 g· L–1 sucrose, 100 mg·L–1 boric acid, 150 mg· L–1 Ca(NO3)2 and 50 mg· L–1 GA3. Low-temperature stress produced the greater inhibition of pollen tube growth compared with high-temperature conditions. The CK2 medium at pH 6.5 resulted in the highest pollen germination rate and most extended pollen tube length. The optimal temperature for storage of dried pollen was –80°C (P < 0.01), and the germination rate was 53.60% after storage for 390 days. Thawing in a 35°C water bath produced the best viability of E. racemosa pollen after storage at –20°C and –80°C. The short-term storage of E. racemosa fresh pollen at 4°C was better than that at –20°C and –80°C (P < 0.01). It is possible to evaluate pollen quality and store pollen grains for E. racemosa by the parameters defined in this study.
Transcriptome and functional analyses reveal ERF053 from Medicago falcata as key regulator in drought resistances
Authors: Li Qian , Jiang Wenbo , Jiang Zhihu , Du Wenxuan , Song Jiaxing , Qiang Zhiquan , Zhang Bo , Pang Yongzhen , Wang Yuxiang
Published: October 2022

Medicago falcata L. is an important legume forage grass with strong drought resistant, which could be utilized as an important gene pool in molecular breed of forage grass. In this study, M. falcata seedlings were treated with 400 mM mannitol to simulate drought stress, and the morphological and physiological changes were investigated, as well as the transcriptome changes of M. falcata seedlings at different treatment time points (0 h, 2 h, 6 h, 12 h, 24 h, 36 h and 48 h). Transcriptome analyses revealed four modules were closely related with drought response in M. falcata by WGCNA analysis, and four ERF transcription factor genes related with drought stress were identified (MfERF053, MfERF9, MfERF034 and MfRAP2.1). Among them, MfERF053 was highly expressed in roots, and MfERF053 protein showed transcriptional activation activity by transient expression in tobacco leaves. Overexpression of MfERF053 in Arabidopsis improved root growth, number of lateral roots and fresh weight under drought, salt stress and exogenous ABA treatments. Transgenic Arabidopsis over-expressing MfERF053 gene grew significantly better than the wild type under both drought stress and salt stress when grown in soil. Taken together, our strategy with transcriptome combined WGCNA analyses identified key transcription factor genes from M. falcata, and the selected MfERF053 gene was verified to be able to enhance drought and salt resistance when over-expressed in Arabidopsis.
The moderating role of transformational leadership on the relationship between deviant workplace behaviors and employee turnover intentions in China
Authors: Qi Linkai , Chaudhary Naveed Iqbal , Yao Kai , Mirza Farhan , Khalid Rabia
Published: October 2022

This study aimed to analyze the effect of deviant workplace behaviors, such as mistreatment, bullying, and incivility on employee turnover intention and identify the transformational leadership role as a moderator. The data was collected through a survey questionnaire with the help of a purposive sampling technique. A total of 318 respondents’ data was gathered from university academic and general staff in China. The results were analyzed through SPSS and structural equation modeling structural equation modeling (SEM) software. The findings indicate that deviant workplace behavior, i.e., mistreatment, bullying, and incivility, significantly affect employee turnover intention. Moreover, a result shows that transformational leadership has a significant moderating role on the relationship between turnover intention and workplace bullying and incivility but was insignificant between turnover intention and workplace mistreatment. Lastly, implications and limitations were also discussed in this article.
Drivers of university–business cooperation of university faculty from the social cognitive theory perspective
Authors: Zhang Hongwei , Chen Xiyue , Lv Yang , Li Mengru
Published: October 2022

As an independent research field, there is growing attention to university–business cooperation (UBC). However, few studies focus on the driving factors of UBC, which remains an open problem in this area. This study analyzes a broad mix of drivers underlying seven UBC activities, namely, curriculum development and design (CDD), student mobility (SD), lifelong learning (LLL), professional mobility (PM), research and development (R&D), commercialization (COM), and entrepreneurship (ENT), and discusses the internal mechanism and external environment of higher education institutions (HEIs) as the moderator variable affecting UBC activities and individual motivations. Specifically, based on the social cognition theory, the independent variables include motivations (money, career, research, education, and social), the internal mechanism (support mechanism, strategic mechanism, and management mechanism), and the external environment (policy environment, economic environment, and cultural environment) are designed. The aforementioned seven UBC activities are taken as dependent variables. This work takes university faculty as the research object. Through empirical analysis, it demonstrates that the combination of driving factors of different UBC activities has its particularity. Furthermore, the results showed that the internal mechanism and external environment of HEIs could positively moderate the relationship between individual motivations and UBC activities. In terms of theoretical contribution, this study reveals the combination of factors that drive university faculty to engage in UBC. On the other hand, it can provide a reference for policymakers and managers to better development of UBC.
Post-error adjustments depend causally on executive attention: Evidence from an intervention study
Authors: Li Qing , Lin Yixuan , Wang Xiangpeng , Zhang Mengke , Stonier Francis , Chen Xu , Chen Antao
Published: October 2022

Detecting and correcting execution errors is crucial for safe and efficient goal-directed behavior. Despite intensive investigations on error processing, the cognitive foundations of this process remain unclear. Based on the presumed relation between executive attention (EA) and error processing, we implemented a seven-day EA intervention by adopting the Posner cueing paradigm to test the potential causal link from EA to error processing in healthy adults. The experimental group (high EA, HEA) was trained on the Posner cueing paradigm, with a ratio of invalid cue (IC) trials to valid cue (VC) trials of 5:1 and a corresponding ratio of 1:1 in the active control group (low EA, LEA). We found that the EA intervention improved EA across intervention sessions. Critically, after the EA intervention, the HEA group showed that post-error accuracy (PEA) was restored to the same level as the post-correct accuracy (in comparison with the LEA group). However, post-error slowing and the flanker effect were not modulated by the EA intervention. Furthermore, we observed that the changes in the accuracy of VC trials positively predicted the changes in PEA and that the two groups were classified according to the changes in PEA with a 61.3% accuracy. Based on these results, we propose that EA causally drives error processing. And the capabilities of the “actively catch” more attention resources and the automatic mismatch processing developed after EA intervention is transferable to error processing, thereby directly resulting in the gains in post-error adjustments. Our work informs the potential cognitive mechanisms underlying this causal link.
The metaverse in education: Definition, framework, features, potential applications, challenges, and future research topics
Authors: Zhang Xinli , Chen Yuchen , Hu Lailin , Wang Youmei
Published: October 2022

The declaration of the COVID-19 pandemic forced humanity to rethink how we teach and learn. The metaverse, a 3D digital space mixed with the real world and the virtual world, has been heralded as a trend of future education with great potential. However, as an emerging item, rarely did the existing study discuss the metaverse from the perspective of education. In this paper, we first introduce the visions of the metaverse, including its origin, definitions, and shared features. Then, the metaverse in education is clearly defined, and a detailed framework of the metaverse in education is proposed, along with in-depth discussions of its features. In addition, four potential applications of the metaverse in education are described with reasons and cases: blended learning, language learning, competence-based education, and inclusive education. Moreover, challenges of the metaverse for educational purposes are also presented. Finally, a range of research topics related to the metaverse in education is proposed for future studies. We hope that, via this research paper, researchers with both computer science and educational technology backgrounds could have a clear vision of the metaverse in education and provide a stepping stone for future studies. We also expect more researchers interested in this topic can commence their studies inspired by this paper.
Skin-to-skin SDF positioning: The key to intersubjective intimacy between mother and very preterm newborn—A pilot matched-pair case-control study
Authors: Buil Aude , Sankey Carol , Caeymaex Laurence , Gratier Maya , Apter Gisèle , Vitte Lisa , Devouche Emmanuel
Published: October 2022

Background

Skin-to-skin contact (SSC) has been widely studied in NICU and several meta-analyses have looked at its benefits, for both the baby and the parent. However, very few studies have investigated SSC’ benefits for communication, in particular in the very-preterm newborn immediately after birth.

Aims

To investigate the immediate benefits of Supported Diagonal Flexion (SDF) positioning during SSC on the quality of mother—very-preterm newborn communication and to examine the coordination of the timing of communicative behaviors, just a few days after birth.

Subjects and study design

Monocentric prospective matched-pair case-control study. Thirty-four mothers and their very preterm infants (27 to 31 + 6 weeks GA, mean age at birth: 30 weeks GA) were assigned to one of the two SSC positioning, either the Vertical Control positioning (n = 17) or the SDF Intervention positioning (n = 17). Mother and newborn were filmed during the first 5 min of their first SSC.

Outcome measures

Infants’ states of consciousness according to the Assessment of Preterm Infants’ Behavior scale (APIB). Onset and duration of newborns’ and mothers’ vocalizations and their temporal proximity within a 1-s time-window.

Results

In comparison with the Vertical group, very preterm newborns in the SDF Intervention Group spent less time in a drowsy state and more in deep sleep. At 3.5 days of life, newborns’ vocal production in SSC did not differ significantly between the two groups. Mothers offered a denser vocal envelope in the SDF group than in the Vertical group and their vocalizations were on average significantly longer. Moreover, in a one-second time-frame, temporal proximity of mother-very preterm newborn behaviors was greater in the SDF Intervention Group.

Conclusion

Although conducted on a limited number of dyads, our study shows that SDF positioning fosters mother-very preterm newborn intimate encounter during the very first skin to skin contact after delivery. Our pioneer data sheds light on the way a mother and her very preterm vocally meet, and constitutes a pilot step in the exploration of innate intersubjectivity in the context of very preterm birth.
Gaze behavior in social interactions between beach volleyball players—An exploratory approach
Authors: Nicklas Andre , Rückel Lisa-Marie , Noël Benjamin , Varga Matyas , Kleinert Jens , Boss Martin , Klatt Stefanie
Published: October 2022

Previous research has indicated that social interactions and gaze behavior analyses in a group setting could be essential tools in accomplishing group objectives. However, only a few studies have examined the impact of social interactions on group dynamics in team sports and their influence on team performance. This study aimed to investigate the effects of game performance pressure on the gaze behavior within social interactions between beach volleyball players during game-like situations. Therefore, 18 expert beach volleyball players conducted a high and a low game performance pressure condition while wearing an eye tracking system. The results indicate that higher game performance pressure leads to more and longer fixation on teammates’ faces. A higher need for communication without misunderstandings could explain this adaptation. The longer and more frequent look at the face could improve the receiving of verbal and non-verbal information of the teammate’s face. Further, players showed inter-individual strategies to cope with high game performance pressure regarding their gaze behavior, for example, increasing the number of fixations and the fixation duration on the teammate’s face. Thereby, this study opens a new avenue for research on social interaction and how it is influenced in/through sport.
Interventions to increase young people's interest in STEM. A scoping review
Authors: Sáinz Milagros , Fàbregues Sergi , Romano María José , López Beatriz-Soledad
Published: October 2022

The underrepresentation of young people and particularly young women in many STEM fields has inspired various intervention programmes and research intended to boost their interest in these areas. The purpose of this scoping review is to examine the characteristics and effectiveness of interventions designed to encourage interest in STEM among secondary school students, particularly female students, over the past 20 years. A systematic search of the literature in five databases and additional search strategies resulted in identifying 215 studies evaluating interventions in different disciplinary fields. Data extraction and synthesis of these studies were carried out, focusing on the methodologies and theoretical foundations used. Twenty-five exemplars were selected to illustrate best practices in designing and evaluating interventions that address the various facets of young people's lack of interest in STEM. These interventions attempt to modify and/or manipulate multiple environmental and school factors to impact students' personal factors associated with STEM interest, such as achievement, self-perception of ability, and self-efficacy. Implications for the design of future interventions and potential outcomes are then discussed.
How do MNEs shape their international strategic alliances to facilitate higher alliance performance? Absorptive capacity as an antecedent
Authors: Chen Zhong , Zheng Langping , Peng Michael Yao-Ping , Shao Lijin
Published: October 2022

The international strategic alliance in international marketing is international social network to realize superior performance. This requires firms to increasingly consolidate international relationship with foreign partners. Knowledge acquisition and integration by absorptive capacity (AC) as an antecedent for international strategic alliance is understudied. This study aims to explore the relationship between AC and international strategic alliance, and their impact on the international performance of multinational enterprises (MNEs). This study empirically verifies the research framework from 223 Taiwanese MNEs. In terms of structural model, researchers conduct PLS-SEM to verify the hypotheses in this study. The results confirm previous studies that indicate a positive relationship among AC, international strategic alliance, and international performance. The results also indicate that international explorative and exploitative alliance fits mediate the relationship between AC and international performance.
The double burden of COVID-19 and Israeli military rule on persons with disabilities in the West Bank of the occupied Palestinian territory
Authors: Mitwalli Suzan , Kiwan Dina , Abdul-Samad Lina , Giacaman Rita
Published: October 2022

Background

The COVID-19 pandemic has enormous negative effects on people’s lives. People with disabilities (PWDs) who have been made vulnerable and who tend to face significant barriers in accessing public services are likely to be affected even more. This study aims to shed light on the impact of the COVID-19 pandemic on PWDs with special attention to their ability to access public services in the West Bank region of the occupied Palestinian territory.

Methods

This qualitative study was conducted between March 2021 and October 2021. The study was divided into two phases. The first phase consisted of interviews with people with different disabilities, while the second phase targeted policymakers and stakeholders in discussion of the results of the first phase. A total of 16 interviews with people with different types of disabilities (7 males, 9 females) were conducted via telephone. For the second phase, a total of 6 interviews were conducted with stakeholders most responsible for addressing the issue of disability and the needs of PWDs in the West Bank. Analytical memos were prepared for all interviews. Main themes and subthemes were identified by reading and re-reading memos and transcripts until themes and subthemes emerged.

Results

All participants agreed that the COVID-19 pandemic had affected all aspects of life for all groups of people, including PWDs. The effects of the pandemic exacerbated the overall living conditions and access to basic services for PWDs. The results show that access to public transportation, public services, and to health services was all interrupted during the pandemic. This was compounded by deterioration of the financial situation for PWDs which further worsened their access. The stakeholders’ interviews confirmed and explained the findings as mainly due to lack of prioritization of PWD’s rights and needs.

Conclusion

The study emphasized that most of the reported barriers to accessing essential services were intensified during the pandemic. Furthermore, the results show that PWDs and their needs are not considered a priority by the Palestinian Authority, exacerbated by the Israeli occupation. Our findings underline the importance of including PWDs in any future crisis planning.
A methodological note on the CYP-guides randomized controlled trial for the treatment of major depression
Authors: Sideridis Georgios , Alkhadim Ghadah
Published: October 2022

Genetically guided therapy for major depression has been recently recommended but has resulted in null effects. We hypothesized that a potential confounding variable for not finding differences in Length of Stay and Re-admission rate in a major depression clinical trial is the equivalence between treatment protocols in the standard treatment (S) and CYP2D6 genotype (G) treatment groups. The two treatment protocols (i.e., type and degree of substrate drugs) were contrasted using a latent class analysis (LCA) model. Specifically, an LCA model specifying the presence of two classes, namely, the G and S groups was estimated with the intercepts of the 30 prescribed drugs freely estimated. This model was compared to a constrained latent class model in which the two treatment protocols (intercept terms) were contrasted to be equivalent between the two treatment groups. Results indicated that there were no significant differences between G and S treatment groups in the types and number of drugs administered. Consequently, the lack of finding significant differences in length of stay and readmission rate may likely be attributed to the equivalence of the treatment protocols.
Training needs and curriculum of continuing medical education among general practitioners in Tibet, China: A cross-sectional survey
Authors: An Kang , Zhang Lin , Qiao Runjuan , Li Caizheng , Zhong Qian , Ma Yiru , Rao Xin , Mao Tingrui , Liu Feng , Zhuo Qiang , She Yi , Li Shuangqing , Su Qiaoli
Published: October 2022

Background

Uneven distribution of health resources is higher in Tibet than in other regions. The development of core professional capability for general practitioners (GPs) is the main goal of continuing medical education (CME) training programs.

Aim

This study aimed to explore the needs of CME for GPs and provide advice for the development of policy, practice, and CME curriculums.

Methods

We conducted a cross-sectional online survey among GPs in Tibet Autonomous Region, China. We designed an online questionnaire including the demographic section, training contents, and training formats about CME.

Results

A total of 108 questionnaires were included in this study. Notably, 79 (73.15%) were women and 56 participants (51.85%) were working in primary care settings. We developed a curriculum priority: first-choice, major alternatives, and secondary considerations. The topics identified as first-choice for CME were related to “cardiovascular disease” (85.19%), “respiratory disease” (81.48%), and “digestive disease” (80.56%). Major alternatives included two essential knowledge and eight clinical skill items. We rated 10 items as secondary considerations. Only 39.81% ranked mental health as an essential priority; bedside teaching (51.85%) was the first choice.

Conclusion

We presented priority areas identified in this study to focus on CME for GPs in Tibet. The 23 topics may reflect the features of general practice, which increasingly require common disease management skills, while a demand-oriented curriculum and staged training plans should be adopted. CME programs should be adapted dynamically to respond to evolving needs.
Association between lifestyle and COVID-19 vaccination: A national cross-sectional study
Authors: Miao Yudong , Zhang Wanliang , Li Yi , Wu Jian , Xu Dongyang , Gu Jianqin , Wang Meiyun , Wei Wei , Ye Beizhu , Miao Chengyuan , Tarimo Clifford Silver , Dong Wenyong
Published: October 2022

Objective

To assess lifestyles, COVID-19 vaccination coverage rates, and the relationships between lifestyles and COVID-19 vaccination among Chinese population.

Methods

We collected data on sociodemographics, perception of the COVID-19 pandemic, lifestyles, and self-reported COVID-19 vaccination via an online survey in China. The chi-square goodness-of-fit test was used to monitor sample saturation throughout the formal online survey. The binary logistic regression analyses were conducted to examine the association between COVID-19 vaccination rate and lifestyle score. We assigned values to 12 lifestyles ranging from positive to negative, with positive lifestyles receiving a higher score and negative lifestyles receiving a lower score, ranging from 1 to 5. For each participant, the total lifestyle scored from 12 to 56. Restricted cubic spline (RCS) was used to visualize the trends and correlations between lifestyle score and COVID-19 vaccination coverage. Propensity score matching (PSM) was used to explore the association between specific lifestyles and COVID-19 vaccination.

Results

A total of 29,925 participants (51.4% females) responded. The lifestyle score of the sample was 44.60 ± 6.13 (scoring range: 12–56). COVID-19 vaccination rate was found to be 89.4% (89.1–89.8%). Female participants reported a higher vaccination rate than male participants (91.5 vs. 87.1%). Compared to Q1, COVID-19 vaccination coverage rates increased with lifestyle total scores [ORQ2 = 1.901 (1.718–2.103), P < 0.001; ORQ3 = 2.373 (2.099–2.684), P < 0.001; and ORQ4 = 3.765 (3.209–4.417), P < 0.001]. After applying PSM, it was determined that all the 12 specific healthy lifestyles analyzed, including maintaining a healthy body weight, a healthy diet, regular physical exercises, adequate sleep, regular physical examination, and others, were found to be positive factors for COVID-19 vaccination.

Conclusion

The majority of mainland Chinese lived a healthy lifestyle throughout the COVID-19 pandemic, and the rate of COVID-19 vaccination was high. Specific healthy lifestyles contributed to COVID-19 vaccination coverage rates significantly. According to the study's findings, global efforts to achieve herd immunity should be prioritized by continually promoting healthy lifestyles and improving public perception of COVID-19 vaccines.
Drugs quality supervision strategy of different distribution channels in pharmaceutical supply chain
Authors: Zhang Siyi , Zhu Lilong
Published: October 2022

Aiming at the dual-channel pharmaceutical supply chain, which consists of two distribution channels, offline medical institutions, and online e-commerce platforms, and taking into account the impact of different strategic choices made by relevant stakeholders on the drugs quality of different distribution channels, this article constructs an evolutionary game model involving the participation of government regulator, pharmaceutical enterprises, medical institutions, and pharmaceutical e-commerce companies. The stable equilibrium points of each participant's strategic choices are solved; the stability of strategic combination is analyzed by Lyapunov's first method, and MATLAB 2020b is used for simulation to verify the influence of each decision variable on the strategic choice of different participants. The results show that, first, the purpose of punishment is to ensure the drugs quality in the pharmaceutical supply chain, but when the fine is too high, it will restrain the economic behavior of pharmaceutical enterprises, which is not conducive to the performance of social responsibilities by other relevant participants. Second, the probability that government regulator strictly supervises the pharmaceutical supply chain and the probability that pharmaceutical enterprises provide high-quality drugs are negatively related to their additional cost. Third, whether medical institutions and pharmaceutical e-commerce companies choose inspection is affected by multiple factors such as inspection cost, sales price, and sales cost. Furthermore, when the penalty for non-inspection of pharmaceutical e-commerce companies is greater than the threshold Fm0, it can ensure that it chooses an inspection strategy. Finally, this article puts forward countermeasures and suggestions on the drugs quality supervision of different distribution channels in the pharmaceutical supply chain.
Is it time we get real? A systematic review of the potential of data-driven technologies to address teachers' implicit biases
Authors: Gauthier Andrea , Rizvi Saman , Cukurova Mutlu , Mavrikis Manolis
Published: October 2022

Data-driven technologies for education, such as artificial intelligence in education (AIEd) systems, learning analytics dashboards, open learner models, and other applications, are often created with an aspiration to help teachers make better, evidence-informed decisions in the classroom. Addressing gender, racial, and other biases inherent to data and algorithms in such applications is seen as a way to increase the responsibility of these systems and has been the focus of much of the research in the field, including systematic reviews. However, implicit biases can also be held by teachers. To the best of our knowledge, this systematic literature review is the first of its kind to investigate what kinds of teacher biases have been impacted by data-driven technologies, how or if these technologies were designed to challenge these biases, and which strategies were most effective at promoting equitable teaching behaviors and decision making. Following PRISMA guidelines, a search of five databases returned n = 359 records of which only n = 2 studies by a single research team were identified as relevant. The findings show that there is minimal evidence that data-driven technologies have been evaluated in their capacity for supporting teachers to make less biased decisions or promote equitable teaching behaviors, even though this capacity is often used as one of the core arguments for the use of data-driven technologies in education. By examining these two studies in conjunction with related studies that did not meet the eligibility criteria during the full-text review, we reveal the approaches that could play an effective role in mitigating teachers' biases, as well as ones that may perpetuate biases. We conclude by summarizing directions for future research that should seek to directly confront teachers' biases through explicit design strategies within teacher tools, to ensure that the impact of biases of both technology (including data, algorithms, models etc.) and teachers are minimized. We propose an extended framework to support future research and design in this area, through motivational, cognitive, and technological debiasing strategies.
Training machine learning with physics-based simulations to predict 2D soil moisture fields in a changing climate
Authors: Leonarduzzi Elena , Tran Hoang , Bansal Vineet , Hull Robert B. , De la Fuente Luis , Bearup Lindsay A. , Melchior Peter , Condon Laura E. , Maxwell Reed M.
Published: October 2022

The water content in the soil regulates exchanges between soil and atmosphere, impacts plant livelihood, and determines the antecedent condition for several natural hazards. Accurate soil moisture estimates are key to applications such as natural hazard prediction, agriculture, and water management. We explore how to best predict soil moisture at a high resolution in the context of a changing climate. Physics-based hydrological models are promising as they provide distributed soil moisture estimates and allow prediction outside the range of prior observations. This is particularly important considering that the climate is changing, and the available historical records are often too short to capture extreme events. Unfortunately, these models are extremely computationally expensive, which makes their use challenging, especially when dealing with strong uncertainties. These characteristics make them complementary to machine learning approaches, which rely on training data quality/quantity but are typically computationally efficient. We first demonstrate the ability of Convolutional Neural Networks (CNNs) to reproduce soil moisture fields simulated by the hydrological model ParFlow-CLM. Then, we show how these two approaches can be successfully combined to predict future droughts not seen in the historical timeseries. We do this by generating additional ParFlow-CLM simulations with altered forcing mimicking future drought scenarios. Comparing the performance of CNN models trained on historical forcing and CNN models trained also on simulations with altered forcing reveals the potential of combining these two approaches. The CNN can not only reproduce the moisture response to a given forcing but also learn and predict the impact of altered forcing. Given the uncertainties in projected climate change, we can create a limited number of representative ParFlow-CLM simulations (ca. 25 min/water year on 9 CPUs for our case study), train our CNNs, and use them to efficiently (seconds/water-year on 1 CPU) predict additional water years/scenarios and improve our understanding of future drought potential. This framework allows users to explore scenarios beyond past observation and tailor the training data to their application of interest (e.g., wet conditions for flooding, dry conditions for drought, etc…). With the trained ML model they can rely on high resolution soil moisture estimates and explore the impact of uncertainties.
The ethics of astrobiology: Humanity’s place in the cosmos and the extinction problem
Authors: Lo Sapio Luca
Published: October 2022

Western philosophy has always stressed the importance of understanding the very nature of the universe and the relationship between humanity and the cosmos. Nowadays, astrobiology is used to shed light on that issue through a set of empirical data. Also, the Fermi paradox and the Rare Earth hypothesis could offer a suitable ground to properly interpret the proofs emerging from space exploration and direct and indirect observations from astronomers, that is, “we are likely the only intelligent species within the universe.” From this, we can say that maybe we are confronted with a cosmic bet. We should better wager on our cosmic importance as a species, otherwise the loss could be immense: the disappearance of the only species through which the universe enabled itself to understand its own secrets and cosmic treasures.
Monitoring mental distress in Para athletes in preparation, during and after the Beijing Paralympic Games 2022: A 22 week prospective mixed-method study
Authors: Bentzen Marte , Kenttä Göran , Karls Tommy , Fagher Kristina
Published: October 2022

It is common in elite sport to monitor athletes' training load, injuries and illnesses, but mental distress is rarely included. An improved understanding of the epidemiology of mental distress among elite Para athletes and how their coaches perceive such monitoring would allow us to better develop and implement preventive measures. The purpose of this study was therefore to (1) prospectively describe elite Para athletes' mental distress, before, during and after the Beijing Paralympic Games (Paralympics Games 22 = PG22); and to (2) gain a better understanding ofifandpotentially howawareness of athletes' mental distress changed, through weekly monitoring, and influenced how coachers perceive athletes' mental distress and if they accounted for this before, during and after PG22. A mixed-method study design was used, in which prospective mental distress (depression and anxiety) data were collected weekly from 13 [Swedish] elite Para athletes in preparation, during and after PG22. Data were screened and evaluated weekly by a physiotherapist and a sports psychologist, and coaches also received weekly reports. A focus-group interview with the coaches were conducted post Paralympics to address coaches' awareness about mental distress and athlete health monitoring in Parasport. For data analyses, descriptive statistics was used for the quantitative data and a content analysis was conducted for the qualitative data. The results reveled the following proportion of datapoints indicating symptoms of anxiety and depression: before PG22 (15.8 and 19.1%); during PG22 (47.6 and 38.2%); and after PG22 (0 and 11.8%). The qualitative results indicated that coaches perceived athlete health monitoring as helpful for increasing their awareness of mental distress, and as a useful tool to initiate support for their athletes as well as improving their coaching. In summary, this cohort of elite Para athletes reported a high proportion of mental distress during the Winter Paralympic Games in Beijing. The results also show that it is important and feasible to monitor Para athletes' mental distress to detect and manage early symptoms of mental distress.
“Central” and “peripheral” adaptation pathways of entangled agrifood systems transformations
Authors: Dagli Winifredo
Published: October 2022

In the agrifood systems of developing countries, local adaptation actions and pathways often interact with other climate and development responses, creating new trade-offs, uncertainties, and potentially maladaptive outcomes. While knowledge on the interacting pathways of adaptation is expanding, previous studies have focused on complex systems dynamics, and studies that address the human, social, and political forces that drive the cascading of risks between different coupled social-ecological systems are few. This paper aims to examine climate adaptation trade-offs, uncertainties, and maladaptation through an interdisciplinary analysis of two interacting pathways of transformational adaptation in the Philippines: the post 2004 disaster rural transformations in the coconut-producing municipality of Infanta and a state-led urban water resilience strategy for the capital region of Metro Manila. Data were collected from January 2021 to March 2022 through ethnographic field visits, participant observation, focus group discussions, semi-structured interviews, geospatial analysis, multicriteria mapping sessions, and review of planning documents and secondary data sources. Key findings suggest that the pathways of transformation and their entanglement are rooted in historical processes of change and that maladaptation is contingent on the political relations between the “central” and “peripheral” pathways. Overall, the paper offers a significant contribution to adaptation research in the agrifood systems of developing countries as it calls for a deeper kind of collective reflexivity and action that can transform narrow notions and practices of resilience and sustainable development.
Transareolar uniportal thoracoscopic extended thymectomy for patients with myasthenia gravis
Authors: Lin Jianbo , Lin Nanlong , Li Xu , Lai Fancai
Published: October 2022

Background

Transareolar uniportal thoracoscopic extended thymectomy (TUTET) has not been previously reported. We attempted to assess the feasibility and safety of TUTET for male myasthenia gravis (MG) patients.

Patients and methods

From February 2013 to February 2020, 46 men with MG underwent TUTET. All patients were followed up for 12–84 months postoperatively by clinic visits or telephone/e-mail interviews.

Results

All surgeries were completed successfully, with an average operation time of 72.6 min. The mean length of transareolar uniportal incision was 3.0 ± 0.4 cm, and the mean postoperative cosmetic score was 3.1 ± 0.5 at discharge. Three months postoperatively, no patients had an apparent surgical scar on the chest wall or complained of postoperative pain. Substantial amelioration of the disease was achieved in a short period, and several benefits were clear. At the 1-year follow-up, all patients showed a good cosmetic effect and high satisfaction.

Conclusions

TUTET is an effective and safe way for men with MG. The uniportal incision is hidden in the areola with sound cosmetic effects. We believe that TUTET is an acceptable procedure for extended thymectomy.
Association of neutropenia at disease onset with severe surgical necrotizing enterocolitis and higher mortality: A retrospective study
Authors: Qin Fanyue , Yuan Mengjie , Zhang Chen , Zhu Chu , Dong Huifang , Xu Falin
Published: October 2022

Background

Neutrophils are among the earliest immune cells recruited to the site of an intestinal injury, but their predictive role in the progression of necrotizing enterocolitis (NEC) has not been fully elucidated. This study aimed to evaluate if a reduction in neutrophils at the onset of NEC is associated with severe surgical NEC and/or NEC-associated deaths.

Methods

This is a retrospective cohort study in which neonates underwent surgery due to NEC during 2015–2020. The data on absolute neutrophil count (ANC), before and at the onset of NEC, were collected from the complete blood count results. The primary exposure was the difference in absolute neutrophil count (ΔANC) at NEC onset. The primary outcome was severe surgical NEC, defined as the residual small bowel length after intestinal resection of <30 cm.

Results

A total of 157 neonates were included in this study, of which 53 were diagnosed with severe surgical NEC. A decrease in ANC at the onset of NEC was associated with an increased probability of severe surgical NEC (crude odds ratio [OR] 1.248, 95% CI 1.107–1.407; P = 0.000). ΔANC (area under the curve [AUC] 0.729, 95% CI 0.653–0.797; P < 0.001] was a good predictor for severe surgical NEC. The addition of platelets to ΔANC at NEC onset (AUC 0.738, 95% CI 0.662–0.808; P < 0.001) resulted in a higher AUC and specificity for severe surgical NEC prediction than ΔANC alone. A reduction in the neutrophil count at NEC onset (ΔANC > 0) was associated with adverse outcomes (hazard ratio [HR] 3.48, 95% CI 1.64–7.36) and a lower survival probability (χ2 10.63; P < 0.001).

Conclusion

A reduction in the ANC at the onset of NEC was associated with severe surgical NEC and higher mortality. The addition of platelets to ΔANC at NEC onset resulted in a higher predictive value of severe surgical NEC. This study may provide a new insight into the bedside evaluation of NEC by analyzing data from the day of NEC onset.
Pulmonary Large Cell Neuroendocrine Carcinoma
Authors: Yang Lan , Fan Ying , Lu Hongyang
Published: October 2022

Pulmonary large cell neuroendocrine carcinoma (LCNEC) is a rare subtype of malignant pulmonary tumor. The incidence rate of LCNEC was reported to be 0.3%–3% in lung cancers. Although LCNEC is classified as non-small cell lung cancer (NSCLC), it is more aggressive and malignant than other NSCLC, and its biological behavior is similar to that of small cell lung cancer (SCLC). Most of the LCNEC patients are elderly smoking male and the clinical manifestations are not specific. The imaging manifestations of the tumors are often located in the periphery and the upper lobes, and the enlargement of mediastinal or hilar lymph nodes is common. The diagnosis is mainly based on pathology by the histological features and immunohistochemistry (IHC). Specific neuroendocrine markers such as chromogranin A (CgA), synaptophysin (Syn) and CD56 are usually diffusely positive in LCNEC, and found that insulinoma-associated protein (INSM1) and high rate of Ki-67 are helpful for diagnosis. More differential diagnoses also increase the difficulty of correctly diagnosing LCNEC. The rise of LCNEC molecular typing in recent years may be helpful for diagnosis and subsequent treatment. This review focuses on the epidemiological features, imaging studies, pathology, diagnosis, treatment, and prognosis of LCNEC.
PotatoMASH—A Low Cost, Genome-Scanning Marker System for Use in Potato Genomics and Genetics Applications
Authors: Leyva-Pérez Maria de la O. , Vexler Lea , Byrne Stephen , Clot Corentin R. , Meade Fergus , Griffin Denis , Ruttink Tom , Kang Jie , Milbourne Dan
Published: October 2022

We have developed PotatoMASH (Potato Multi-Allele Scanning Haplotags), a novel low-cost, genome-scanning marker platform. We designed a panel of 339 multi-allelic regions placed at 1 Mb intervals throughout the euchromatic portion of the genome. These regions were assayed using a multiplex amplicon sequencing approach, which allows for genotyping hundreds of plants at a cost of 5 EUR/sample. We applied PotatoMASH to a population of over 700 potato lines. We obtained tetraploid dosage calls for 2012 short multi-allelic haplotypes in 334 loci, which ranged from 2 to 14 different haplotypes per locus. The system was able to diagnose the presence of targeted pest-resistance markers, to detect quantitative trait loci (QTLs) by genome-wide association studies (GWAS) in a tetraploid population, and to track variation in a diploid segregating population. PotatoMASH efficiently surveys genetic variation throughout the potato genome, and can be implemented as a single low-cost genotyping platform that will allow the routine and simultaneous application of marker-assisted selection (MAS) and other genotyping applications in commercial potato breeding programmes.