bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibition Effect of Kinetic Hydrate Inhibitors on the Growth of Methane Hydrate in Gas–Liquid Phase Separation State
Authors: Cheng Liwei , Wang Limin , Li Zhi , Liu Bei , Chen Guangjin
Published: November 2019

The effect of kinetic hydrate inhibitors (KHIs) on the growth of methane hydrate in the gas–liquid phase separation state is studied at the molecular level. The simulation results show that the kinetic inhibitors, named PVP and PVP-A, show good inhibitory effects on the growth of methane hydrate under the gas–liquid phase separation state, and the initial position of the kinetic hydrate inhibitors has a major effect on the growth of methane hydrates. In addition, inhibitors at different locations exhibit different inhibition performances. When the inhibitor molecules are located at the gas–liquid phase interface, increasing the contact area between the groups of the inhibitor molecules and methane is beneficial to enhance the inhibitory performance of the inhibitors. When inhibitor molecules are located at the solid–liquid phase interface, the inhibitor molecules adsorbed on the surface of the hydrate nucleus and decreased the direct contact of hydrate nucleus with the surrounding water and methane molecules, which would delay the growth of hydrate nucleus. Moreover, the increase of hydrate surface curvature and the Gibbs–Thomson effect caused by inhibitors can also reduce the growth rate of methane hydrate.
Study on Deformation and Energy Release Characteristics of Overlying Strata under Different Mining Sequence in Close Coal Seam Group Based on Similar Material Simulation
Authors: Cui Feng , Jia Chong , Lai Xingping
Published: November 2019

For the characteristics of overburden deformation and energy release under different mining sequences of close-distance coal seam groups, physical material similar simulation experiments were carried out, and comprehensive monitoring methods such as dial gauge, total station, micro-seismic monitor, and pressure sensor were used to test the Guangou Coal Mine. The comparative analysis of the initial mining and the upward recovery of the B4-1 coal seam is carried out to study the migration law, mine pressure distribution, and energy release characteristics of the overlying strata during W1145 mining face mining and residual coal mining. The results show that the maximum subsidence of surface and rock formation caused by re-mining of B4-1 residual coal is 0.96 m and 2.57 m respectively, which is 0.42 m and 0.47 m lower than that of W1145 working face. The boundary angle, moving angle, and rock stratum formed by the upward recovery of the remaining coal seam are 79.3°, 81.1°, and 67.5° respectively, which are smaller than the 80.9°, 82.3°, and 75.8° formed by the first mining. The cumulative development height of the fracture zone caused by upward mining is 115.7 m, which is 8.0% smaller than the cumulative development height of the downstream fracture zone of 125.8 m. When the up-level mining is carried out, the fragmentation effect of the rock layer below the key layer is strong, which makes the loosely broken rock block have a better supporting effect. Therefore, the residual coal mining time is longer than that of the first mining. The initial pressure step of the residual coal recovery is 139.2 m, and the average step of the cycle is 34.2 m, which is significantly larger than the 128.0 m and 26.0 m of the first mining. The loose rock strata that are disturbed by the upward recovery are more likely to be broken. Therefore, there are more micro-seismic events during the re-mining of the remaining coal. The B4-1 residual coals have a total of 945 incidents of re-seismic micro-seismic events, which is 292 more than the W1145 working face. After the B2 coal seam mining disturbance, the energy of some rock layers above the B4-1 coal seam is released, so that the micro-seismic energy caused by re-mining of the remaining coal seam is small. Through microseismic monitoring, it can be concluded that the accumulated energy in the process of upward re-mining of remaining coal seam is less than that in the process of downward mining of W1145 working face. Upward recovery is more likely to cause damage in the disturbed loose rock formation. Therefore, the frequency of micro-seismic events during the upward recovery is higher, and the partial energy release of the rock after the disturbance is caused, so that the source energy generated in the unit length of the upward recovery is smaller than the initial one.
A Game-Theory Method to Design Job Rotation Schedules to Prevent Musculoskeletal Disorders Based on Workers’ Preferences and Competencies
Authors: Asensio-Cuesta Sabina , García-Gómez Juan M. , Poza-Luján José-Luis , Conejero J. Alberto
Published: November 2019

Job rotation is an organizational strategy based on the systematic exchange of workers between jobs in a planned manner according to specific criteria. This study presents the GS-Rot method, a method based on Game Theory, in order to design job rotation schedules by considering not only workers’ job preferences, but also the competencies required for different jobs. With this approach, we promote workers’ active participation in the design of the rotation plan. It also let us deal with restrictions in assigning workers to job positions according to their disabilities (temporal or permanent). The GS-Rot method has been implemented online and applied to a case in a work environment characterized by the presence of a high repetition of movements, which is a significant risk factor associated with work-related musculoskeletal disorders (WMSDs). A total of 17 workstations and 17 workers were involved in the rotation, four of them with physical/psychological limitations. Feasible job rotation schedules were obtained in a short time (average time 27.4 milliseconds). The results indicate that in the rotations driven by preference priorities, almost all the workers (94.11%) were assigned to one of their top five preferences. Likewise, 48.52% of job positions were assigned to workers in their top five of their competence lists. When jobs were assigned according to competence, 58.82% of workers got an assignment among their top five competence lists. Furthermore, 55.87% of the workers achieved jobs in their top five preferences. In both rotation scenarios, the workers varied performed jobs, and fatigue accumulation was balanced among them. The GS-Rot method achieved feasible and uniform solutions regarding the workers’ exposure to job repetitiveness.
The Effect of Treatment during A Haze/Post-Haze Year on Subsequent Respiratory Morbidity Status among Successful Treatment Tuberculosis Cases
Authors: Suyanto Suyanto , Geater Alan , Chongsuvivatwong Virasakdi
Published: November 2019

The purpose of this study was to evaluate the respiratory morbidity status within the two to three years among successful (completed/cured) treatment of tuberculosis cases during a haze year (2015) and a post-haze year (2016). The study was conducted among 133 cases of a 2015 group and 103 cases of a 2016 group between January to March 2018 in Pekanbaru city, Indonesia. The St George Respiratory Questionnaire (SGRQ) was used to assess respiratory morbidity status. A higher score corresponds to worse respiratory morbidity. Based on a directed acyclic graph, quantile regression models were constructed to assess the associations between haze/post-haze year and the SGRQ (symptom, activity, impact, and total) domains score. The subsequent respiratory morbidity status of tuberculosis (TB) cases was poorer among respondents treated during a haze year (2015). Among SGRQ domains, only the activity domain score showed significant difference, in which the median for the 2015 group was 23.7 (inter-quartile range (IQR); 17.2, 30.9) compared to 18.4 (IQR; 11.9, 24.8) for the 2016 group. The effect was limited to the 2015 group who were exposed by an average PM10 index ≥ 55 during TB treatment. This raises concern for monitoring and improving the quality of life of TB patients treated during a haze year.
Distribution, Sources and Water Quality Evaluation of the Riverine Solutes: A Case Study in the Lancangjiang River Basin, Tibetan Plateau
Authors: Liu Jinke , Han Guilin , Liu Man , Zeng Jie , Liang Bin , Qu Rui
Published: November 2019

To examine the chemical composition, potential sources of solutes, and water quality of Lancangjiang River, the concentrations of major ions (Ca2+, Mg2+, Na+, K+, HCO3−, SO42−, Cl− and NO3−) in 45 river water samples collected in July and August 2019 were determined. Ca2+ and HCO3− are the predominant ions in river water. The extremely low K+ and NO3− concentrations and the sparse population suggest that the anthropogenic inputs are limited. The Pearson correlation coefficients and the elemental ratios Ca2+/Na+ versus Mg2+/Na+, Ca2+/Na versus HCO3−/Na+, [Ca2+ + Mg2+]/[HCO3−] versus [SO42−]/[HCO3−] reveal the mixing processes of different sources; the chemical composition of the river water is controlled by the mixture of carbonate weathering, evaporite weathering and silicate weathering inputs. To quantify the contributions of atmospheric input and rock dissolution, the forward method is employed in this study, which is based on the mass balance equation. The calculation results suggest the carbonate weathering inputs and gypsum dissolution make up the majority of the riverine cations, while silicate weathering and halite dissolution constitutes a relatively small proportion, the contributions of the atmospheric input are limited. The fast dissolution rate of evaporite and carbonate minerals and their lithologic distributions should be the key factor. To evaluate the water quality for drinking and irrigation purposes, the drinking water quality guidelines and the calculated parameters were employed, including sodium adsorption ratio (SAR), soluble sodium percentage (Na%,) and residual sodium carbonate (RSC). The assessments indicate that the river waters in the middle-lower reaches are generally suitable for irrigation and drinking purpose, and will not lead to health and soil problems, such as soil compaction and salinization. While in the upper reaches, the dissolution of carbonate and gypsum minerals transport abundant ions into river water and the river waters are not appropriate to use directly. This result highlights that the water quality status can also be affected by natural weathering processes in the area without anthropogenic inputs, where the long-time monitoring of water quality is also necessary.
How Energy Consumption and Pollutant Emissions Affect the Disparity of Public Health in Countries with High Fossil Energy Consumption
Authors: Xing Xinpeng , Wang Jianhua , Liu Tiansen , Liu He , Zhu Yue
Published: November 2019

Public health issues are a global focus, but recent research on the links between fossil energy consumption, pollutant emissions, and public health in different regions have presented inconsistent conclusions. In order to quantify the effect of fossil energy use and pollutant emissions on public health from the global perspective, this paper investigates 33 countries with high GDP and fossil energy consumption from 1995 to 2015 using a fixed effect model. Further, this paper utilizes heterogeneity analysis to characterize the disparity of countries with different features. Empirical results indicate that total fossil energy consumption is beneficial to the life expectancy of the population (LEP), but pollutant emissions (PM10 concentration and greenhouse gas scale) have a negative effect on LEP. Moreover, the heterogeneity test indicates that pollutant emissions lowers LEP in net energy importers more than in net energy exporters, and the effect of such emissions in low- and middle-income countries on public health is more harmful than that in high-income countries. These findings suggest that it is a greater priority for governments to strengthen the control of pollutant emissions through enhancing the efficiency of energy consumption, rather than by reducing its scale of use in low- and middle-income, and net energy importing countries. Additionally, governments also need to focus on the volatility of pollutant emissions in high-income countries with necessary control measures.
Satisfaction with the Aesthetic Effect and Quality of Life for Women after Breast Conserving Therapy (BCT)—Preliminary Research
Authors: Krzos Agata , Stanisławek Andrzej , Jędrych Marian , Łuczyk Marta , Ślusarska Barbara
Published: November 2019

All methods of breast cancer treatment may potentially lead to breast deformities, which are often associated with the reduced mental well-being of patients. Breast conserving therapy (BCT) is commonly used, and its core element is breast conserving surgery (BCS).The aim of this study was to determine the level of satisfaction with the aesthetic outcome of surgery and quality of life (QoL) of breast cancer patients undergoing BCT in a longitudinal study performed three months, six months and 12 months after surgery. This longitudinal observational study was carried out on a group of 91 women. The Breast-QTM BCT 1.0 questionnaire was used in this study. Before surgery, patients assessed their satisfaction with the appearance of their breasts (SwB) at an average level of M = 56.0. Satisfaction with the aesthetic outcome (SwO) of BCS was highest among all patients three months after surgery (M = 63.0). The mean score in the sub-scale of psychosocial well-being (Psycho-soc W-B) before surgery was M = 62.0, while in the subsequent periods of the study, it was higher. The mean score for physical well-being (Physical W-B) before surgery was M = 69.92; and in the subsequent study periods, it was lower. The level of patient satisfaction with the outcome of the surgery and the QoL related to health do not differ significantly in post-operative observation. QoL in terms of psychosocial functioning in patients undergoing BCT is significantly higher 12 months after surgery compared to the pre-operative period. Patient satisfaction with the BCS aesthetic outcome is positively related to the evaluation of QoL in terms of psychosocial functioning.
Geochemical Baseline Values Determination and Evaluation of Heavy Metal Contamination in Soils of Lanping Mining Valley (Yunnan Province, China)
Authors: Li Zuran , Deblon Judith , Zu Yanqun , Colinet Gilles , Li Bo , He Yongmei
Published: November 2019

The largest lead/zinc mine in China is located in Lanping mining valley. The real impact of mining activity on the Lanping mining valley has not been studied to date. This study aims to characterize the geochemical baseline values and risk assessment of heavy metal contamination in soils of a study area located in the Lanping mining valley including upstream, mining and downstream areas. The results showed that the mean soil pH value was 6.8, and organic matter was 34.3%, in surface layer of the mining area. The mean soil pH value in the upstream and downstream areas was less than 5.5. The concentrations of Pb and Zn in the mining area were 56 and 47 times above the world average, the concentrations of Pb, Zn and Cd in the upstream area were six, seven, and six times above the world average, and the concentrations of Pb, Zn and Cd in the downstream area were eight, eight, and 18 times above the world average, respectively. The proposed geochemical baseline values of Pb, Cu, Zn and Cd were 169.93, 31.81, 569.06 and 4.13 mg·kg−1, respectively. The pseudo total and ethylene diamine tetraacetic acid (EDTA)-extractable concentrations of Pb, Zn and Cd showed similar tendency as follows: mining area > downstream area > upstream area. The contamination degree with the geoaccumulation index (Igeo) and the improved Nemerow index (IIN) in the upstream and mining areas was non-contamination or slight contamination with low or moderate risk with the individual ecological risk index (Er) and the comprehensive potential ecological risk index (RI), although moderate or heavy contamination with pollution factor (Pi) and the Nemerow index (IN). The contamination degree with Igeo and IIN in the downstream area was non-contamination or extreme contamination with low or extreme risk with Er and RI. The results suggest that the IIN should be recommended to assess the soil contamination of heavy metals and the geochemical baseline values would be important for the environmental management and remediation of soils contaminated by heavy metals.
Stepping Up: Predictors of ‘Stepping’ within an iCBT Stepped-Care Intervention for Depression
Authors: Nicholas Jennifer , Ringland Kathryn E. , Graham Andrea K. , Knapp Ashley A. , Lattie Emily G. , Kwasny Mary J. , Mohr David C.
Published: November 2019

Internet-based cognitive behavioral therapy (iCBT) may overcome barriers to mental health care and has proven efficacious. However, this approach currently exists outside the existing mental health care delivery system. Stepped care is a proposed framework for integrating digital mental health (DMH) into health systems by initiating iCBT and “stepping up” care to a more intensive intervention should iCBT prove ineffective. This study explores pre-treatment factors associated with reaching stepping criteria among patients receiving iCBT. This exploratory analysis of a stepped care arm of a larger randomized trial examined participants who were stepped to a more intensive intervention if they did not respond to iCBT. The association of pre-treatment factors on stepping were examined using Kruskal–Wallis and Chi-square tests. One-fifth of the 151 participants met criteria for stepping within the 20-week treatment period. Only pre-treatment depression severity and treatment preference were associated with increased likelihood of stepping (p = 0.049 and 0.048, respectively). The low number of individuals who stepped provides support for iCBT as an effective, low intensity treatment for depression. The modest association of pre-treatment depression and preference to not receive iCBT may be useful in identifying patients who are less likely to respond.
Community Stakeholders’ Perspectives on Intimate Partner Violence during Pregnancy—A Qualitative Study from Ethiopia
Authors: Gashaw Bosena , Magnus Jeanette , Schei Berit , Solbraekke Kari
Published: November 2019

Intimate partner violence (IPV) in pregnancy adversely affects the health of women and unborn children. To prevent this, the community responses, societal systems, and structures to support victims of IPV in pregnancy are vital. Objectives: to explore community stakeholders’ perspectives related to IPV in pregnancy in Jimma, Ethiopia, and if needed, create the knowledge base for interventions. Methods: using an exploratory design, this qualitative study had a maximum-variation (multiple spectrum sources) sampling strategy with 16 semi-structured interviews of purposively selected key informants representing different community institutions. Guided by Connell’s theory of gender and power, a content analysis of the translated interviews was conducted using Atlas.ti 7 software. Results: reconciliation between IPV victims and their abusers was the solution promoted by almost all the respondents. There was limited awareness of the adverse impacts IPV in pregnancy has on the health of the woman and the foetus. Despite regular encounters with victims, there is no organized or structured operational response to support IPV victims between the participating institutions. Conclusion: the potential danger of IPV for the mother or the unborn child was not well understood by the members of the studied Ethiopian community. Neither coordinated efforts to support IPV victims nor links among relevant agencies existed. The study demonstrated the dire need of coordinated practical action, changes in current socio-cultural norms, formal training and capacity building, awareness creation, clear intervention guidelines, and facilitation of support networks among relevant institutions in Ethiopian communities.
A ‘Control Model’ of Social Media Engagement in Adolescence: A Grounded Theory Analysis
Authors: Throuvala Melina A. , Griffiths Mark D. , Rennoldson Mike , Kuss Daria J.
Published: November 2019

Adolescents actively use social media, which engages them cognitively, emotionally, and behaviorally. However, the underlying psychological mechanisms of engagement have not been adequately addressed. The present study examined adolescents’ psychological processes as these develop in their everyday interactions via social media. The sample comprised six focus groups with 42 adolescents from UK-based schools. Data were analyzed using constructivist grounded theory. The resulting concepts related to individual, social, and structurally related processes, highlighting a synergy between the processes underlying use and a gradual reduction of control as individual, social, and structurally led processes emerge, conceptualized as the ‘control model’ of social media engagement. The findings highlight a controlling aspect in engagement and a dynamic interplay between the processes as mutually determining the quality and the intensity of the interaction. Recommendations are provided for examining control as a main emotional, cognitive, and behavioral mechanism in problematic and/or addictive social media and smartphone use.
A Potent Autophagy Inhibitor (Lys05) Enhances the Impact of Ionizing Radiation on Human Lung Cancer Cells H1299
Authors: Cechakova Lucie , Ondrej Martin , Pavlik Vojtech , Jost Petr , Cizkova Dana , Bezrouk Ales , Pejchal Jaroslav , Amaravadi Ravi K. , Winkler Jeffrey D. , Tichy Ales
Published: November 2019

Autophagy inhibition through small-molecule inhibitors is one of the approaches to increase the efficiency of radiotherapy in oncological patients. A new inhibitor—Lys05—with the potential to accumulate within lysosomes and to block autophagy was discovered a few years ago. Several studies have addressed its chemosensitizing effects but nothing is known about its impact in the context of ionizing radiation (IR). To describe its role in radiosensitization, we employed radioresistant human non-small cell lung carcinoma cells (H1299, p53-negative). Combined treatment of H1299 cells by Lys05 together with IR decreased cell survival in the clonogenic assay and real-time monitoring of cell growth more than either Lys05 or IR alone. Immunodetection of LC3 and p62/SQSTM1 indicated that autophagy was inhibited, which correlated with increased SQSTM1 and decreased BNIP3 gene expression determined by qRT-PCR. Fluorescence microscopy and flow cytometry uncovered an accumulation of lysosomes. Similarly, transmission electron microscopy demonstrated the accumulation of autophagosomes confirming the ability of Lys05 to potentiate autophagy inhibition in H1299 cells. We report here for the first time that Lys05 could be utilized in combination with IR as a promising future strategy in the eradication of lung cancer cells.
Genome-Wide Transcriptome Analysis of Rice Seedlings after Seed Dressing with Paenibacillus yonginensis DCY84T and Silicon
Authors: Yoo Yo-Han , Kim Minjae , Chandran Anil Kumar Nalini , Hong Woo-Jong , Ahn Hye Ryun , Lee Gang Taik , Kang Sungju , Suh Dabin , Kim Jin-O , Kim Yeon-Ju , Jung Ki-Hong
Published: November 2019

Plant-growth-promoting bacteria (PGPB) are beneficial microorganisms that can also protect against disease and environmental stress. Silicon (Si) is the second most abundant element in soil, and is known to increase plant growth, grain yield, resistance to biotic stress, and tolerance to abiotic stress. Combined treatment of PGPB and Si has been shown to further enhance plant growth and crop yield. To determine the global effects of the PGPB and Si on rice growth, we compared rice plants treated with Paenibacillus yonginensis DCY84T (DCY84T) and Si with untreated rice. To identify the genes that respond to DCY84T+Si treatment in rice, we performed an RNA-Seq transcriptome analysis by sampling treated and untreated roots on a weekly basis for three weeks. Overall, 576 genes were upregulated, and 394 genes were downregulated in treated roots, using threshold fold-changes of at least 2 (log2) and p-values < 0.05. Gene ontology analysis showed that phenylpropanoids and the L-phenylalanine metabolic process were prominent in the upregulated genes. In a metabolic overview analysis using the MapMan toolkit, pathways involving phenylpropanoids and ethylene were strongly associated with upregulated genes. The functions of seven upregulated genes were identified as being associated with drought stress through a literature search, and a stress experiment confirmed that plants treated with DCY84T+Si exhibited greater drought tolerance than the untreated control plants. Furthermore, the predicted protein–protein interaction network analysis associated with DCY84T+ Si suggests mechanisms underlying growth promotion and stress tolerance.
ABCC3 Expressed by CD56dim CD16+ NK Cells Predicts Response in Glioblastoma Patients Treated with Combined Chemotherapy and Dendritic Cell Immunotherapy
Authors: Pellegatta Serena , Di Ianni Natalia , Pessina Sara , Paterra Rosina , Anghileri Elena , Eoli Marica , Finocchiaro Gaetano
Published: November 2019

Recently, we found that temozolomide (TMZ) can upregulate the expression of the multidrug-resistance protein ABCC3 in NK cells from both glioma-bearing mice and glioblastoma patients treated with dendritic cell immunotherapy combined with TMZ, allowing NK cells to escape apoptosis and favoring their role as antitumor effector cells. Here, we demonstrate that CD56dim NK cells expressing CD16+ are predominant in patients surviving more than 12 months after surgery without disease progression. CD56dim CD16+ NK cells co-expressed high levels of ABCC3 and IFN-γ. Notably, not only basal but also TMZ-induced ABCC3 expression was related to a strong, long-term NK cell response and a better prognosis of patients. The identification of the single nucleotide polymorphism (SNP) rs35467079 with the deletion of a cytosine (−897DelC) in the promoter region of the ABCC3 gene resulted associated with a better patient outcome. ABCC3 expression in patients carrying DelC compared to patients with reference haplotype was higher and modulated by TMZ. The transcription factor NRF2, involved in ABCC3 induction, was phosphorylated in CD56dim CD16+ NK cells expressing ABCC3 under TMZ treatment. Thus, ABCC3 protein and the SNP −897DelC can play a predictive role in patients affected by GBM, and possibly other cancers, treated with dendritic cell immunotherapy combined with chemotherapy.
Static Solid Relaxation Ordered Spectroscopy: SS-ROSY
Authors: Boutis Gregory S. , Kausik Ravinath
Published: November 2019

A two-dimensional pulse sequence is introduced for correlating nuclear magnetic resonance anisotropic chemical shifts to a relaxation time (e.g., T1) in solids under static conditions. The sequence begins with a preparatory stage for measuring relaxation times, and is followed by a multiple pulse sequence for homonuclear dipolar decoupling. Data analysis involves the use of Fourier transform, followed by a one-dimensional inverse Laplace transform for each frequency index. Experimental results acquired on solid samples demonstrate the general approach, and additional variations involving heteronuclear decoupling and magic angle spinning are discussed.
Combined Effect of Cadmium and Lead on Durum Wheat
Authors: Aprile Alessio , Sabella Erika , Francia Enrico , Milc Justyna , Ronga Domenico , Pecchioni Nicola , Ferrari Erika , Luvisi Andrea , Vergine Marzia , De Bellis Luigi
Published: November 2019

Cadmium (Cd) and lead (Pb) are two toxic heavy metals (HMs) whose presence in soil is generally low. However, industrial and agricultural activities in recent years have significantly raised their levels, causing progressive accumulations in plant edible tissues, and stimulating research in this field. Studies on toxic metals are commonly focused on a single metal, but toxic metals occur simultaneously. The understanding of the mechanisms of interaction between HMs during uptake is important to design agronomic or genetic strategies to limit contamination of crops. To study the single and combined effect of Cd and Pb on durum wheat, a hydroponic experiment was established to examine the accumulation of the two HMs. Moreover, the molecular mechanisms activated in the roots were investigated paying attention to transcription factors (bHLH family), heavy metal transporters and genes involved in the biosynthesis of metal chelators (nicotianamine and mugineic acid). Cd and Pb are accumulated following different molecular strategies by durum wheat plants, even if the two metals interact with each other influencing their respective uptake and translocation. Finally, we demonstrated that some genes (bHLH 29, YSL2, ZIF1, ZIFL1, ZIFL2, NAS2 and NAAT) were induced in the durum wheat roots only in response to Cd.
The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues
Authors: Soni Nikul , Ross Alastair B. , Scheers Nathalie , Nookaew Intawat , Gabrielsson Britt G. , Sandberg Ann-Sofie
Published: November 2019

Excess energy intake can trigger an uncontrolled inflammatory response, leading to systemic low-grade inflammation and metabolic disturbances that are hypothesised to contribute to cardiovascular disease and type 2 diabetes. The long chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are suggested to mitigate this inflammatory response, but the mechanisms are unclear, especially at the tissue level. Adipose tissues, the first tissues to give an inflammatory response, may be an important target site of action for EPA and DHA. To evaluate the effects of EPA and DHA in white and brown adipose tissues, we fed male C57Bl/6J mice either a high fat diet (HFD) with 5% corn oil, an HFD with 40% of the corn oil substituted for purified EPA and DHA triglycerides (HFD-ED), or normal chow, for 8 weeks. Fatty acid profiling and transcriptomics were used to study how EPA and DHA affect retroperitoneal white and brown adipose tissues. HFD-ED fed mice showed reduced lipid accumulation and levels of the pro-inflammatory fatty acid arachidonic acid in both white and brown adipose tissues, compared with HFD-corn oil fed animals. The transcriptomic analysis showed changes in β-oxidation pathways, supporting the decreased lipid accumulation in the HFD-ED fed mice. Therefore, our data suggests that EPA and DHA supplementation of a high fat diet may be anti-inflammatory, as well as reduce lipid accumulation in adipose tissues.
Advances in Sphingolipidoses: CRISPR-Cas9 Editing as an Option for Modelling and Therapy
Authors: Santos Renato , Amaral Olga
Published: November 2019

Sphingolipidoses are inherited genetic diseases characterized by the accumulation of glycosphingolipids. Sphingolipidoses (SP), which usually involve the loss of sphingolipid hydrolase function, are of lysosomal origin, and represent an important group of rare diseases among lysosomal storage disorders. Initial treatments consisted of enzyme replacement therapy, but, in recent decades, various therapeutic approaches have been developed. However, these commonly used treatments for SP fail to be fully effective and do not penetrate the blood–brain barrier. New approaches, such as genome editing, have great potential for both the treatment and study of sphingolipidoses. Here, we review the most recent advances in the treatment and modelling of SP through the application of CRISPR-Cas9 genome editing. CRISPR-Cas9 is currently the most widely used method for genome editing. This technique is versatile; it can be used for altering the regulation of genes involved in sphingolipid degradation and synthesis pathways, interrogating gene function, generating knock out models, or knocking in mutations. CRISPR-Cas9 genome editing is being used as an approach to disease treatment, but more frequently it is utilized to create models of disease. New CRISPR-Cas9-based tools of gene editing with diminished off-targeting effects are evolving and seem to be more promising for the correction of individual mutations. Emerging Prime results and CRISPR-Cas9 difficulties are also discussed.
Sphingolipids in Obesity and Correlated Co-Morbidities: The Contribution of Gender, Age and Environment
Authors: Torretta Enrica , Barbacini Pietro , Al-Daghri Nasser M. , Gelfi Cecilia
Published: November 2019

This paper reviews our present knowledge on the contribution of ceramide (Cer), sphingomyelin (SM), dihydroceramide (DhCer) and sphingosine-1-phosphate (S1P) in obesity and related co-morbidities. Specifically, in this paper, we address the role of acyl chain composition in bodily fluids for monitoring obesity in males and females, in aging persons and in situations of environmental hypoxia adaptation. After a brief introduction on sphingolipid synthesis and compartmentalization, the node of detection methods has been critically revised as the node of the use of animal models. The latter do not recapitulate the human condition, making it difficult to compare levels of sphingolipids found in animal tissues and human bodily fluids, and thus, to find definitive conclusions. In human subjects, the search for putative biomarkers has to be performed on easily accessible material, such as serum. The serum “sphingolipidome” profile indicates that attention should be focused on specific acyl chains associated with obesity, per se, since total Cer and SM levels coupled with dyslipidemia and vitamin D deficiency can be confounding factors. Furthermore, exposure to hypoxia indicates a relationship between dyslipidemia, obesity, oxygen level and aerobic/anaerobic metabolism, thus, opening new research avenues in the role of sphingolipids.
Transcriptome Analysis of Salt-Sensitive and Tolerant Genotypes Reveals Salt-Tolerance Metabolic Pathways in Sugar Beet
Authors: Geng Gui , Lv Chunhua , Stevanato Piergiorgio , Li Renren , Liu Hui , Yu Lihua , Wang Yuguang
Published: November 2019

Soil salinization is a common environmental problem that seriously affects the yield and quality of crops. Sugar beet (Beta vulgaris L.), one of the main sugar crops in the world, shows a strong tolerance to salt stress. To decipher the molecular mechanism of sugar beet under salt stress, we conducted transcriptomic analyses of two contrasting sugar beet genotypes. To the best of our knowledge, this is the first comparison of salt-response transcriptomes in sugar beet with contrasting genotypes. Compared to the salt-sensitive cultivar (S710), the salt-tolerant one (T710MU) showed better growth and exhibited a higher chlorophyll content, higher antioxidant enzyme activity, and increased levels of osmotic adjustment molecules. Based on a high-throughput experimental system, 1714 differentially expressed genes were identified in the leaves of the salt-sensitive genotype, and 2912 in the salt-tolerant one. Many of the differentially expressed genes were involved in stress and defense responses, metabolic processes, signal transduction, transport processes, and cell wall synthesis. Moreover, expression patterns of several genes differed between the two cultivars in response to salt stress, and several key pathways involved in determining the salt tolerance of sugar beet, were identified. Our results revealed the mechanism of salt tolerance in sugar beet and provided potential metabolic pathways and gene markers for growing salt-tolerant cultivars.
Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells
Authors: Wu Ti-Hui , Chang Shan-Yueh , Shih Yu-Lueng , Huang Tsai-Wang , Chang Hung , Lin Ya-Wen
Published: November 2019

Cisplatin is still the primary therapeutic choice for advanced lung cancers without driver mutations. The occurrence of cisplatin resistance is a major clinical problem in lung cancer treatment. The natural extracted agent emetine reportedly has anticancer effects. This study aimed to explore the possible role of emetine in cisplatin resistance. We used cell viability, Western blot, and Wnt reporter assays to show that emetine suppresses proliferation, β-catenin expression, and Wnt/β-catenin signaling in non-small cell lung cancer (NSCLC). The synergism of emetine and cisplatin was assessed by constructing isobolograms and calculating combination index (CI) values using the Chou-Talalay method. Emetine effectively synergized with cisplatin to suppress the proliferation of cancer cells. Furthermore, nuclear β-catenin and cancer stem cell-related markers were upregulated in the cisplatin-resistant subpopulation of CL1-0 cells. Emetine enhanced the anticancer efficacy of cisplatin and synergized with cisplatin in the cisplatin-resistant subpopulation of CL1-0 cells. Taken together, these data suggest that emetine could suppress the growth of NSCLC cells through the Wnt/β-catenin pathway and contribute to a synergistic effect in combination with cisplatin.
Comparative Transcriptomics of Flammulina filiformis Suggests a High CO2 Concentration Inhibits Early Pileus Expansion by Decreasing Cell Division Control Pathways
Authors: Yan Jun-Jie , Tong Zong-Jun , Liu Yuan-Yuan , Li Yi-Ning , Zhao Chen , Mukhtar Irum , Tao Yong-Xin , Chen Bing-Zhi , Deng You-Jin , Xie Bao-Gui
Published: November 2019

Carbon dioxide is commonly used as one of the significant environmental factors to control pileus expansion during mushroom cultivation. However, the pileus expansion mechanism related to CO2 is still unknown. In this study, the young fruiting bodies of a popular commercial mushroom Flammulina filiformis were cultivated under different CO2 concentrations. In comparison to the low CO2 concentration (0.05%), the pileus expansion rates were significantly lower under a high CO2 concentration (5%). Transcriptome data showed that the up-regulated genes enriched in high CO2 concentration treatments mainly associated with metabolism processes indicated that the cell metabolism processes were active under high CO2 conditions. However, the gene ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with cell division processes contained down-regulated genes at both 12 h and 36 h under a high concentration of CO2. Transcriptome and qRT-PCR analyses demonstrated that a high CO2 concentration had an adverse effect on gene expression of the ubiquitin–proteasome system and cell cycle–yeast pathway, which may decrease the cell division ability and exhibit an inhibitory effect on early pileus expansion. Our research reveals the molecular mechanism of inhibition effects on early pileus expansion by elevated CO2, which could provide a theoretical basis for a CO2 management strategy in mushroom cultivation.
Transplacental Gene Delivery (TPGD) as a Noninvasive Tool for Fetal Gene Manipulation in Mice
Authors: Nakamura Shingo , Watanabe Satoshi , Ando Naoko , Ishihara Masayuki , Sato Masahiro
Published: November 2019

Transplacental gene delivery (TPGD) is a technique for delivering nucleic acids to fetal tissues via tail-vein injections in pregnant mice. After transplacental transport, administered nucleic acids enter fetal circulation and are distributed among fetal tissues. TPGD was established in 1995 by Tsukamoto et al., and its mechanisms, and potential applications have been further characterized since. Recently, discoveries of sequence specific nucleases, such as zinc-finger nuclease (ZFN), transcription activator-like effector nucleases (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) (CRISPR/Cas9), have revolutionized genome editing. In 2019, we demonstrated that intravenous injection of plasmid DNA containing CRISPR/Cas9 produced indels in fetal myocardial cells, which are comparatively amenable to transfection with exogenous DNA. In the future, this unique technique will allow manipulation of fetal cell functions in basic studies of fetal gene therapy. In this review, we describe developments of TPGD and discuss their applications to the manipulation of fetal cells.
IL-1β Induces SOCS2 Expression in Human Dendritic Cells
Authors: Sarajlic Muamera , Neuper Theresa , Föhrenbach Quiroz Kim Tamara , Michelini Sara , Vetter Julia , Schaller Susanne , Horejs-Hoeck Jutta
Published: November 2019

Dendritic cells (DCs) regulate immunity and inflammation and respond to various stimuli, including cytokines. IL-1β is a key cytokine in the course of both acute and chronic inflammatory responses, making it indispensable for protection of the host, but also linking it to several diseases. Thus, IL-1β signaling must be tightly regulated. As suppressor of cytokine signaling (SOCS) proteins effectively control immune responses, we investigated the role of SOCS2 in IL-1β-induced DC activation. Human monocyte-derived DCs were stimulated with IL-1β, and SOCS2 mRNA and protein levels were measured. DC activation was assessed by cytokine secretion and surface marker expression. For functional analysis, small interfering RNA (siRNA)-based SOCS2 silencing was performed. SOCS2 expression was also analyzed in a curated NCBI GEO dataset of myeloid leukemia patients. We found IL-1β to be a potent inducer of SOCS2 expression. By silencing SOCS2, we showed that SOCS2 specifically limits IL-1β-induced IL-8 secretion. Moreover, our analysis revealed that SOCS2 levels are significantly increased in patients with acute and chronic myeloid leukemia, two hematological malignancies where disease progression is closely linked to IL-1β. This study identifies SOCS2 as a novel IL-1β-inducible target gene and points toward a potential role of SOCS2 in IL-1β-mediated DC activation.
Software-Based Three-Dimensional Deconvolution Microscopy of Cytoskeletal Proteins in Cultured Fibroblast Using Open-Source Software and Open Hardware
Authors: Katoh Kazuo
Published: November 2019

As conventional fluorescence microscopy and confocal laser scanning microscopy generally produce images with blurring at the upper and lower planes along the z-axis due to non-focal plane image information, the observation of biological images requires “deconvolution.” Therefore, a microscope system’s individual blur function (point spread function) is determined theoretically or by actual measurement of microbeads and processed mathematically to reduce noise and eliminate blurring as much as possible. Here the author describes the use of open-source software and open hardware design to build a deconvolution microscope at low cost, using readily available software and hardware. The advantage of this method is its cost-effectiveness and ability to construct a microscope system using commercially available optical components and open-source software. Although this system does not utilize expensive equipment, such as confocal and total internal reflection fluorescence microscopes, decent images can be obtained even without previous experience in electronics and optics.
Composite Plate Rolling Technology of 304/Q345R Based on a Corrugated Interface
Authors: Wang Shun , Zhao Guanghui , Li Yugui , Li Juan , Song Yaohui
Published: November 2019

A new rolling process of the 304/Q345R composite plate based on a corrugated interface was developed. Through numerical simulation and rolling experiments, the metal deformation law, the stress and strain field distribution, and the bond strength of the corrugated plate were studied. By comparison, the corrugated interface effectively increased the length of the composite interface. Also, the composite interface from the 2D corrugated surface became a 3D corrugated surface, and a combination of the 304/Q345R composite plate metallurgy was achieved with a low reduction rate. Simultaneously, the interface bonding strength was improved.
Coherent THz Hyper-Raman: Spectroscopy and Application in THz Detection
Authors: Ceraso Arianna , Mou Sen , Rubano Andrea , Paparo Domenico
Published: November 2019

Recently we have demonstrated a new nonlinear optical effect in the THz interval of frequencies. The latter is based on the use of femtosecond optical pulses and intense, sub-ps, broadband terahertz (THz) pulses to generate a THz-optical four- and five-wave mixing in the investigated material. The spectrum of the generated signal is resolved in time and wavelength and displays two pronounced frequency sidebands, Stokes and anti-Stokes, close to the optical second harmonic central frequency 2 ω L , where ω L is the optical central frequency of the fundamental beam, thus resembling the spectrum of standard hyper-Raman scattering, and hence we named this effect ‘THz hyper-Raman’—THYR. We applied this technique to several crystalline materials, including α-quartz and gallium selenide. In the first material, we find that the THYR technique brings spectroscopic information on a large variety of low-energy excitations that include polaritons and phonons far from the Γ-point, which are difficult to study with standard optical techniques. In the second example, we show that this new tool offers some advantages in detecting ultra-broadband THz pulses. In this paper we review these two recent results, showing the potentialities of this new THz technique.
Feasibility of Using 3D Printed Polyvinyl Alcohol (PVA) for Creating Self-Healing Vascular Tunnels in Cement System
Authors: Li Zijing , Souza Lívia Ribeiro de , Litina Chrysoula , Markaki Athina E. , Al-Tabbaa Abir
Published: November 2019

Pursuing long-term self-healing infrastructures has gained popularity in the construction field. Vascular networks have the potential to achieve long-term self-healing in cementitious infrastructures. To avoid further monitoring of non-cementitious tubes, sacrificial material can be used as a way of creating hollow channels. In this research, we report a new method for fabrication of complex 3D internal hollow tunnels using 3D printing of polyvinyl alcohol (PVA). The behaviour of 3D printed PVA structures in cement pastes was investigated using computed-tomography (CT) combined with X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy with energy dispersive spectroscopy (SEM/EDX). Results showed that (i) 1300 min were needed to fully dissolve 1 g of a 3D printed PVA structure, and different pH solutions did not significantly change the PVA dissolving process compared with a neutral environment; (ii) a low water/cement ratio can minimize early stage cracking resulting from PVA expansion; (iii) and PVA-cement interaction products were mainly calcite and a Ca-polymer compound. In conclusion, controlling the PVA expansion by decreasing the water/cement (w/c) ratio provides a promising approach to achieve 3D hollow channels in cement and, therefore, makes it possible to create complex tunnels within self-healing cementitious materials.
Analysis of Important Fabrication Factors That Determine the Sensitivity of MWCNT/Epoxy Composite Strain Sensors
Authors: Hwang Mun-Young , Kang Lae-Hyong
Published: November 2019

Composite sensors based on carbon nanotubes have been leading to significant research providing interesting aspects for realizing cost-effective and sensitive piezoresistive strain sensors. Here, we report a wide range of piezoresistive performance investigations by modifying fabrication factors such as multi-wall carbon nanotubes (MWCNT) concentration and sensor dimensions for MWCNT/epoxy composites. The resistance change measurement analyzed the influence of the fabrication factors on the changes in the gauge factor. The dispersion quality of MWCNTs in the epoxy polymer matrix was investigated by scanning electron microscopy (SEM) images and conductivity measurement results. A configuration circuit was designed to use the composite sensor effectively. It has been shown that, in comparison with commercially available strain gauges, composites with CNT fillers have the potential to attain structural health monitoring capabilities by utilizing the variation of electrical conductivity and its relation to strain or damage within the composite. Based on the characteristics of the MWCNT, we predicted the range of conductivity that can be seen in the fabricated composite. The sensor may require a large surface area and a thin thickness as fabrication factors at minimum filler concentration capable of exhibiting a tunneling effect, in order to fabricate a sensor with high sensitivity. The proposed composite sensors will be suitable in various potential strain sensor applications, including structural health monitoring.