bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA
Authors: Aviñó Anna , Mazzini Stefania , Gargallo Raimundo , Eritja Ramon
Published: February 2016

Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
Novel Triazole-Quinoline Derivatives as Selective Dual Binding Site Acetylcholinesterase Inhibitors
Authors: Mantoani Susimaire , Chierrito Talita , Vilela Adriana , Cardoso Carmen , Martínez Ana , Carvalho Ivone
Published: February 2016

Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder worldwide. Currently, the only strategy for palliative treatment of AD is to inhibit acetylcholinesterase (AChE) in order to increase the concentration of acetylcholine in the synaptic cleft. Evidence indicates that AChE also interacts with the β-amyloid (Aβ) protein, acting as a chaperone and increasing the number and neurotoxicity of Aβ fibrils. It is known that AChE has two binding sites: the peripheral site, responsible for the interactions with Aβ, and the catalytic site, related with acetylcholine hydrolysis. In this work, we reported the synthesis and biological evaluation of a library of new tacrine-donepezil hybrids, as a potential dual binding site AChE inhibitor, containing a triazole-quinoline system. The synthesis of hybrids was performed in four steps using the click chemistry strategy. These compounds were evaluated as hAChE and hBChE inhibitors, and some derivatives showed IC50 values in the micro-molar range and were remarkably selective towards hAChE. Kinetic assays and molecular modeling studies confirm that these compounds block both catalytic and peripheral AChE sites. These results are quite interesting since the triazole-quinoline system is a new structural scaffold for AChE inhibitors. Furthermore, the synthetic approach is very efficient for the preparation of target compounds, allowing a further fruitful new chemical library optimization.
Characterization of Nanoparticle Dispersion in Red Blood Cell Suspension by the Lattice Boltzmann-Immersed Boundary Method
Authors: Tan Jifu , Keller Wesley , Sohrabi Salman , Yang Jie , Liu Yaling
Published: February 2016

Nanodrug-carrier delivery in the blood stream is strongly influenced by nanoparticle (NP) dispersion. This paper presents a numerical study on NP transport and dispersion in red blood cell (RBC) suspensions under shear and channel flow conditions, utilizing an immersed boundary fluid-structure interaction model with a lattice Boltzmann fluid solver, an elastic cell membrane model and a particle motion model driven by both hydrodynamic loading and Brownian dynamics. The model can capture the multiphase features of the blood flow. Simulations were performed to obtain an empirical formula to predict NP dispersion rate for a range of shear rates and cell concentrations. NP dispersion rate predictions from the formula were then compared to observations from previous experimental and numerical studies. The proposed formula is shown to accurately predict the NP dispersion rate. The simulation results also confirm previous findings that the NP dispersion rate is strongly influenced by local disturbances in the flow due to RBC motion and deformation. The proposed formula provides an efficient method for estimating the NP dispersion rate in modeling NP transport in large-scale vascular networks without explicit RBC and NP models.
Biomolecules and Natural Medicine Preparations: Analysis of New Sources of Bioactive Compounds from Ribes and Rubus spp. Buds
Authors: Donno Dario , Mellano Maria , Cerutti Alessandro , Beccaro Gabriele
Published: February 2016

It is well known that plants are important sources for the preparation of natural remedies as they contain many biologically active compounds. In particular, polyphenols, terpenic compounds, organic acids, and vitamins are the most widely occurring groups of phytochemicals. Some endemic species may be used for the production of herbal preparations containing phytochemicals with significant bioactivity, as antioxidant activity and anti-inflammatory capacities, and health benefits. Blackberry sprouts and blackcurrant buds are known to contain appreciable levels of bioactive compounds, including flavonols, phenolic acids, monoterpenes, vitamin C, and catechins, with several clinical effects. The aim of this research was to perform an analytical study of blackcurrant and blackberry bud-preparations, in order to identify and quantify the main biomarkers, obtaining a specific phytochemical fingerprint to evaluate the single botanical class contribution to total phytocomplex and relative bioactivity, using a High Performance Liquid Chromatograph−Diode Array Detector; the same analyses were performed both on the University laboratory and commercial preparations. Different chromatographic methods were used to determine concentrations of biomolecules in the preparations, allowing for quantification of statistically significant differences in their bioactive compound content both in the case of Ribes nigrum and Rubus cultivated varieties at different harvest stages. In blackcurrant bud-extracts the most important class was organic acids (50.98%) followed by monoterpenes (14.05%), while in blackberry preparations the main bioactive classes were catechins (50.06%) and organic acids (27.34%). Chemical, pharmaceutical and agronomic-environmental knowledge could be important for obtaining label certifications for the valorization of specific genotypes, with high clinical and pharmaceutical value: this study allowed to develop an effective tool for the natural preparation quality control and bioactivity evaluation through the chemical fingerprinting of bud preparations.
Efficient Emulation of Radiative Transfer Codes Using Gaussian Processes and Application to Land Surface Parameter Inferences
Authors: Gómez-Dans José , Lewis Philip , Disney Mathias
Published: February 2016

There is an increasing need to consistently combine observations from different sensors to monitor the state of the land surface. In order to achieve this, robust methods based on the inversion of radiative transfer (RT) models can be used to interpret the satellite observations. This typically results in an inverse problem, but a major drawback of these methods is the computational complexity. We introduce the concept of Gaussian Process (GP) emulators: surrogate functions that accurately approximate RT models using a small set of input (e.g., leaf area index, leaf chlorophyll, etc.) and output (e.g., top-of-canopy reflectances or at sensor radiances) pairs. The emulators quantify the uncertainty of their approximation, and provide a fast and easy route to estimating the Jacobian of the original model, enabling the use of e.g., efficient gradient descent methods. We demonstrate the emulation of widely used RT models (PROSAIL and SEMIDISCRETE) and the coupling of vegetation and atmospheric (6S) RT models targetting particular sensor bands. A comparison with the full original model outputs shows that the emulators are a viable option to replace the original model, with negligible bias and discrepancies which are much smaller than the typical uncertainty in the observations. We also extend the theory of GP to cope with models with multivariate outputs (e.g., over the full solar reflective domain), and apply this to the emulation of PROSAIL, coupled 6S and PROSAIL and to the emulation of individual spectral components of 6S. In all cases, emulators successfully predict the full model output as well as accurately predict the gradient of the model calculated by finite differences, and produce speed ups between 10,000 and 50,000 times that of the original model. Finally, we use emulators to invert leaf area index ( L A I ), leaf chlorophyll content ( C a b ) and equivalent leaf water thickness ( C w ) from a time series of observations from Sentinel-2/MSI, Sentinel-3/SLSTR and Proba-V observations. We use sophisticated Hamiltonian Markov Chain Monte Carlo (MCMC) methods that exploit the speed of the emulators as well as the gradient estimation, a variational data assimilation (DA) method that extends the problem with temporal regularisation, and a particle filter using a regularisation model. The variational and particle filter approach appear more successful (meaning parameters closer to the truth, and smaller uncertainties) than the MCMC approach as a result of using the temporal regularisation mode. These work therefore suggests that GP emulators are a practical way to implement sophisticated parameter retrieval schemes in an era of increasing data volumes.
EnGeoMAP 2.0—Automated Hyperspectral Mineral Identification for the German EnMAP Space Mission
Authors: Mielke Christian , Rogass Christian , Boesche Nina , Segl Karl , Altenberger Uwe
Published: February 2016

Algorithms for a rapid analysis of hyperspectral data are becoming more and more important with planned next generation spaceborne hyperspectral missions such as the Environmental Mapping and Analysis Program (EnMAP) and the Japanese Hyperspectral Imager Suite (HISUI), together with an ever growing pool of hyperspectral airborne data. The here presented EnGeoMAP 2.0 algorithm is an automated system for material characterization from imaging spectroscopy data, which builds on the theoretical framework of the Tetracorder and MICA (Material Identification and Characterization Algorithm) of the United States Geological Survey and of EnGeoMAP 1.0 from 2013. EnGeoMAP 2.0 includes automated absorption feature extraction, spatio-spectral gradient calculation and mineral anomaly detection. The usage of EnGeoMAP 2.0 is demonstrated at the mineral deposit sites of Rodalquilar (SE-Spain) and Haib River (S-Namibia) using HyMAP and simulated EnMAP data. Results from Hyperion data are presented as supplementary information.
Understanding the Spatial Temporal Vegetation Dynamics in Rwanda
Authors: Ndayisaba Felix , Guo Hao , Bao Anming , Guo Hui , Karamage Fidele , Kayiranga Alphonse
Published: February 2016

Knowledge of current vegetation dynamics and an ability to make accurate predictions of ecological changes are essential for minimizing food scarcity in developing countries. Vegetation trends are also closely related to sustainability issues, such as management of conservation areas and wildlife habitats. In this study, AVHRR and MODIS NDVI datasets have been used to assess the spatial temporal dynamics of vegetation greenness in Rwanda under the contrasting trends of precipitation, for the period starting from 1990 to 2014, and for the first growing season (season A). Based on regression analysis and the Hurst exponent index methods, we have investigated the spatial temporal characteristics and the interrelationships between vegetation greenness and precipitation in light of NDVI and gridded meteorological datasets. The findings revealed that the vegetation cover was characterized by an increasing trend of a maximum annual change rate of 0.043. The results also suggest that 81.3% of the country’s vegetation has improved throughout the study period, while 14.1% of the country’s vegetation degraded, from slight (7.5%) to substantial (6.6%) deterioration. Most pixels with severe degradation were found in Kigali city and the Eastern Province. The analysis of changes per vegetation type highlighted that five types of vegetation are seriously endangered: The “mosaic grassland/forest or shrubland” was severely degraded, followed by “sparse vegetation,” “grassland or woody vegetation regularly flooded on water logged soil,” “artificial surfaces” and “broadleaved forest regularly flooded.” The Hurst exponent results indicated that the vegetation trend was consistent, with a sustainable area percentage of 40.16%, unsustainable area of 1.67% and an unpredictable area of 58.17%. This study will provide government and local authorities with valuable information for improving efficiency in the recently targeted countrywide efforts of environmental protection and regeneration.
Block Sparse Compressed Sensing of Electroencephalogram (EEG) Signals by Exploiting Linear and Non-Linear Dependencies
Authors: Mahrous Hesham , Ward Rabab
Published: February 2016

This paper proposes a compressive sensing (CS) method for multi-channel electroencephalogram (EEG) signals in Wireless Body Area Network (WBAN) applications, where the battery life of sensors is limited. For the single EEG channel case, known as the single measurement vector (SMV) problem, the Block Sparse Bayesian Learning-BO (BSBL-BO) method has been shown to yield good results. This method exploits the block sparsity and the intra-correlation (i.e., the linear dependency) within the measurement vector of a single channel. For the multichannel case, known as the multi-measurement vector (MMV) problem, the Spatio-Temporal Sparse Bayesian Learning (STSBL-EM) method has been proposed. This method learns the joint correlation structure in the multichannel signals by whitening the model in the temporal and the spatial domains. Our proposed method represents the multi-channels signal data as a vector that is constructed in a specific way, so that it has a better block sparsity structure than the conventional representation obtained by stacking the measurement vectors of the different channels. To reconstruct the multichannel EEG signals, we modify the parameters of the BSBL-BO algorithm, so that it can exploit not only the linear but also the non-linear dependency structures in a vector. The modified BSBL-BO is then applied on the vector with the better sparsity structure. The proposed method is shown to significantly outperform existing SMV and also MMV methods. It also shows significant lower compression errors even at high compression ratios such as 10:1 on three different datasets.
Post-Seismic Deformation from the 2009 Mw 6.3 Dachaidan Earthquake in the Northern Qaidam Basin Detected by Small Baseline Subset InSAR Technique
Authors: Liu Yang , Xu Caijun , Wen Yangmao , Li Zhicai
Published: February 2016

On 28 August 2009, one thrust-faulting Mw 6.3 earthquake struck the northern Qaidam basin, China. Due to the lack of ground observations in this remote region, this study presents high-precision and high spatio-temporal resolution post-seismic deformation series with a small baseline subset InSAR technique. At the temporal scale, this changes from fast to slow with time, with a maximum uplift up to 7.4 cm along the line of sight 334 days after the event. At the spatial scale, this is more obvious at the hanging wall than that at the footwall, and decreases from the middle to both sides at the hanging wall. We then propose a method to calculate the correlation coefficient between co-seismic and post-seismic deformation by normalizing them. The correlation coefficient is found to be 0.73, indicating a similar subsurface process occurring during both phases. The results indicate that afterslip may dominate the post-seismic deformation during 19–334 days after the event, which mainly occurs with the fault geometry and depth similar to those of the c-seismic rupturing, and partly extends to the shallower and deeper depths.
An Improved STARFM with Help of an Unmixing-Based Method to Generate High Spatial and Temporal Resolution Remote Sensing Data in Complex Heterogeneous Regions
Authors: Xie Dengfeng , Zhang Jinshui , Zhu Xiufang , Pan Yaozhong , Liu Hongli , Yuan Zhoumiqi , Yun Ya
Published: February 2016

Remote sensing technology plays an important role in monitoring rapid changes of the Earth's surface. However, sensors that can simultaneously provide satellite images with both high temporal and spatial resolution haven’t been designed yet. This paper proposes an improved spatial and temporal adaptive reflectance fusion model (STARFM) with the help of an Unmixing-based method (USTARFM) to generate the high spatial and temporal data needed for the study of heterogeneous areas. The results showed that the USTARFM had higher accuracy than STARFM methods in two aspects of analysis: individual bands and of heterogeneity analysis. Taking the predicted NIR band as an example, the correlation coefficients (r) for the USTARFM, STARFM and unmixing methods were 0.96, 0.95, 0.90, respectively (p-value < 0.001); Root Mean Square Error (RMSE) values were 0.0245, 0.0300, 0.0401, respectively; and ERGAS values were 0.5416, 0.6507, 0.8737, respectively. The USTARM showed consistently higher performance than STARM when the degree of heterogeneity ranged from 2 to 10, highlighting that the use of this method provides the capacity to solve the data fusion problems faced when using STARFM. Additionally, the USTARFM method could help researchers achieve better performance than STARFM at a smaller window size from its heterogeneous land surface quantitative representation.
Vision-Based Pose Estimation for Robot-Mediated Hand Telerehabilitation
Authors: Airò Farulla Giuseppe , Pianu Daniele , Cempini Marco , Cortese Mario , Russo Ludovico , Indaco Marco , Nerino Roberto , Chimienti Antonio , Oddo Calogero , Vitiello Nicola
Published: February 2016

Vision-based Pose Estimation (VPE) represents a non-invasive solution to allow a smooth and natural interaction between a human user and a robotic system, without requiring complex calibration procedures. Moreover, VPE interfaces are gaining momentum as they are highly intuitive, such that they can be used from untrained personnel (e.g., a generic caregiver) even in delicate tasks as rehabilitation exercises. In this paper, we present a novel master–slave setup for hand telerehabilitation with an intuitive and simple interface for remote control of a wearable hand exoskeleton, named HX. While performing rehabilitative exercises, the master unit evaluates the 3D position of a human operator’s hand joints in real-time using only a RGB-D camera, and commands remotely the slave exoskeleton. Within the slave unit, the exoskeleton replicates hand movements and an external grip sensor records interaction forces, that are fed back to the operator-therapist, allowing a direct real-time assessment of the rehabilitative task. Experimental data collected with an operator and six volunteers are provided to show the feasibility of the proposed system and its performances. The results demonstrate that, leveraging on our system, the operator was able to directly control volunteers’ hands movements.
A Path Analysis of the Effects of Principal Professional Orientation towards Leadership, Professional Teacher Behavior, and School Academic Optimism on School Reading Achievement
Authors: Mitchell Roxanne , Tarter C.
Published: February 2016

This study tested the effects of the principal’s professional orientation towards leadership/enabling school structure (ESS) on two mediating variables, school academic optimism (SAO) and professional teacher behavior (PTB), on the outcome variable school reading achievement (RA). Data were drawn from a sample of 54 schools (including 45 elementary schools and nine middle schools); the school was the unit of analysis. Data analysis supported a path to RA in which a structural variable, ESS was the immediate antecedent of SAO and PTB. Two control variables, school level and SES, were included in the model. SES had a significant effect on SAO but not on PTB. School level had a negative effect on both PTB and SAO suggesting that both variables were higher in elementary school and declined in middle school. SES paired with SAO in predicting RA. As expected, SAO had a greater effect on RA than SES. The significance of the findings lies in the confirmation of SAO as an important influence on RA and in demonstrating the importance of ESS in establishing a context in which AO and PTB can flourish.
Vertical Jumping Tests versus Wingate Anaerobic Test in Female Volleyball Players: The Role of Age
Authors: Nikolaidis Pantelis , Afonso Jose , Clemente-Suarez Vicente , Alvarado Jose , Driss Tarak , Knechtle Beat , Torres-Luque Gema
Published: February 2016

Single and continuous vertical jumping tests, as well as the Wingate anaerobic test (WAnT), are commonly used to assess the short-term muscle power of female volleyball players; however, the relationship among these tests has not been studied adequately. Thus, the aim of the present study was to examine the relationship of single and continuous vertical jumps with the WAnT in female volleyball players. Seventy adolescent (age 16.0 ± 1.0 years, body mass 62.5 ± 7.1 kg, height 170.4 ± 6.1 cm, body fat 24.2% ± 4.3%) and 108 adult female volleyball players (age 24.8 ± 5.2 years, body mass 66.5 ± 8.7 kg, height 173.2 ± 7.4 cm, body fat 22.0% ± 5.1%) performed the squat jump (SJ), countermovement jump (CMJ), Abalakov jump (AJ), 30 s Bosco test and WAnT (peak power, Ppeak; mean power, Pmean). Mean power in the Bosco test was correlated (low to large magnitude) with Pmean of the WAnT (r = 0.27, p = 0.030 in adolescents versus r = 0.56, p < 0.001 in adults). SJ, CMJ and AJ also correlated with Ppeak (0.28 ≤ r ≤ 0.46 in adolescents versus 0.58 ≤ r ≤ 0.61 in adults) and with Pmean (0.43 ≤ r ≤ 0.51 versus 0.67 ≤ r ≤ 0.71, respectively) of the WAnT (p < 0.05). In summary, the impact of the Bosco test and WAnT on muscle power varied, especially in the younger age group. Single jumping tests had larger correlations with WAnT in adults than in adolescent volleyball players. These findings should be taken into account by volleyball coaches and fitness trainers during the assessment of short-term muscle power of their athletes.
Proactive Environmental Strategy, Technological Eco-Innovation and Firm Performance—Case of Poland
Authors: Ryszko Adam
Published: February 2016

Nowadays, scholars exploring the relationship between proactive environmental strategy and firm competitiveness do not focus on the question of whether it pays to be green but rather investigate when, for whom or how it does so. This paper analyzes the links between proactive environmental strategy, technological eco-innovation and firm performance. In particular, it examines the influence of proactive environmental strategy on firm performance and explores this relationship through technological eco-innovation. A research model has been developed and tested using a sample of 292 firms operating in Poland. In order to test the proposed research model and hypotheses, structural equation modeling using partial least squares has been employed. The findings do not confirm that proactive environmental strategy directly affects firm performance. However, the results show the significant mediating role of technological eco-innovation in this relationship. Since the technological eco-innovation reduces environmental impact and improves business performance, this research proves that it simultaneously contributes to environmental and economic pillars of sustainable development.
Apoptosis Activation in Human Lung Cancer Cell Lines by a Novel Synthetic Peptide Derived from Conus californicus Venom
Authors: Oroz-Parra Irasema , Navarro Mario , Cervantes-Luevano Karla , Álvarez-Delgado Carolina , Salvesen Guy , Sanchez-Campos Liliana , Licea-Navarro Alexei
Published: February 2016

Lung cancer is one of the most common types of cancer in men and women and a leading cause of death worldwide resulting in more than one million deaths per year. The venom of marine snails Conus contains up to 200 pharmacologically active compounds that target several receptors in the cell membrane. Due to their diversity and specific binding properties, Conus toxins hold great potential as source of new drugs against cancer. We analyzed the cytotoxic effect of a 17-amino acid synthetic peptide (s-cal14.1a) that is based on a native toxin (cal14.1a) isolated from the sea snail Conus californicus. Cytotoxicity studies in four lung cancer cell lines were complemented with measurement of gene expression of apoptosis-related proteins Bcl-2, BAX and the pro-survival proteins NFκB-1 and COX-2, as well as quantification of caspase activity. Our results showed that H1299 and H1437 cell lines treated with s-call4.1a had decreased cell viability, activated caspases, and reduced expression of the pro-survival protein NFκB-1. To our knowledge, this is the first report describing activation of apoptosis in human lung cancer cell lines by s-cal14.1a and we offer insight into the possible mechanism of action.
A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target
Authors: Bernáldez Johanna , Jiménez Samanta , González Luis , Ferro Jesús , Soto Enrique , Salceda Emilio , Chávez Daniela , Aguilar Manuel , Licea-Navarro Alexei
Published: February 2016

A novel conotoxin, named as PiVIIA, was isolated from the venom of Conus princeps, a marine predatory cone snail collected in the Pacific Southern Coast of Mexico. Chymotryptic digest of the S-alkylated peptide in combination with liquid chromatography coupled to tandem mass spectrometry, were used to define the sequencing of this peptide. Eleven N-terminal amino acids were verified by automated Edman degradation. PiVIIA is a 25-mer peptide (CDAOTHYCTNYWγCCSGYCγHSHCW) with six cysteine residues forming three disulphide bonds, a hydroxyproline (O) and two gamma carboxyglutamic acid (γ) residues. Based on the arrangement of six Cys residues (C-C-CC-C-C), this conotoxin might belong to the O2-superfamily. Moreover, PiVIIA has a conserved motif (-γCCS-) that characterizes γ-conotoxins from molluscivorous Conus. Peptide PiVIIA has 45% sequence identity with γ-PnVIIA—the prototype of this family. Biological activity of PiVIIA was assessed by voltage-clamp recording in rat dorsal root ganglion neurons. Perfusion of PiVIIA in the µM range produces a significant increase in the Ca2+ currents, without significantly modifying the Na+, K+ or proton-gated acid sensing ionic currents. These results indicate that PiVIIA is a new conotoxin whose activity deserves further studies to define its potential use as a positive modulator of neuronal activity.
String-Inspired Gravity through Symmetries
Authors: Belinchón José
Published: February 2016

We study a string-inspired cosmological model from the symmetries point of view. We start by deducing the form that each physical quantity must take so that the field equations, in the string frame, admit self-similar solutions. In the same way, we formalize the use of power-law solutions (less restrictive than the self-similar ones) by studying the wave equation for the dilaton through the Lie group method. Furthermore, we show how to generate more solutions by using this approach. As examples, we calculate exact solutions to several cosmological models in the four-dimensional NS-NS (Neveu-Schwarz-Neveu-Schwarz) sector of low-energy effective string theory coupled to a dilaton and an axion-like H-field within the string frame background, with FRW and the Bianchi Type II metrics. We also study the existence of Noether symmetries, which allow us to determine the form of the physical quantities in the framework of FRW geometry and to find exact cosmological solutions.
Efficiency of a Horizontal Sub-Surface Flow Constructed Wetland Treatment System in an Arid Area
Authors: Albalawneh Abeer , Chang Tsun-Kuo , Chou Chi-Su , Naoum Sireen
Published: February 2016

The main objective of this study was to evaluate the performance and treatment efficiency of the Horizontal Sub-Surface Flow Constructed Wetland treatment system (HSF-CW) in an arid climate. Seventeen sub-surface, horizontal-flow HSF-CW units have been operated for approximately three years to improve the quality of partially-treated municipal wastewater. The studied design parameters included two sizes of volcanic tuff media (i.e., fine or coarse), two different bed dimensions (i.e., long and short), and three plantation types (i.e., reed, kenaf, or no vegetation as a control). The effluent Biological Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), Total Suspended Solid (TSS), and phosphorus from all of the treatments were significantly lower as compared to the influent and demonstrated a removal efficiency of 55%, 51%, 67%, and 55%, respectively. There were significant increases in Electrical Conductivity (EC), sulfate, and calcium in the effluent of most HSF-CWs due to evaporative concentration and mineral dissolution from the media. The study suggests that unplanted beds with either fine or coarse media are the most suitable combinations among all of the studied designs based on their treatment efficiency and less water loss in arid conditions.
Estimating Sediment Discharge Using Sediment Rating Curves and Artificial Neural Networks in the Shiwen River, Taiwan
Authors: Tfwala Samkele , Wang Yu-Min
Published: February 2016

Sediment in river is usually transported during extreme events related to intense rainfall and high river flows. The conventional means of collecting data in such events are risky and costly compared to water discharge measurements. Hence, the lack of sediment data has prompted the use of sediment rating curves (SRC). The aim of this study is to explore the abilities of artificial neural networks (ANNs) in advancing the precision of stream flow-suspended discharge relationships during storm events in the Shiwen River, located in southern Taiwan. The ANNs used were multilayer perceptrons (MLP), the coactive neurofuzzy inference system model (CANFISM), time lagged recurrent networks (TLRN), fully recurrent neural networks (FRNN) and the radial basis function (RBF). A comparison is made between SRC and the ANNs. Hourly based water and sediment discharge during 8 storms were manually collected and used as inputs for the SRC and the ANNs. Results have shown that the ANN models were superior in reproducing hourly sediment discharge compared to SRC. The findings further suggest that MLP can provide the most accurate estimates of sediment discharge, (R2 of 0.903) compared to CANFISM, TLRN, FRNN and RBF. SRC had the lowest R2 (0.765), and resulted in underestimations of peak sediment discharge (−47%).
La villa gallo-romaine de Fréthun
Authors: Demarez Jean-Daniel
Published: February 2016

Het Gallo-Romeinse stadje Fréthun ziet eruit als een klein landbouwbedrijf waarvan men maar twee gebouwen ontdekt heeft. Het woongebouw dat tijdens de Flavische période op een waarschijnlijk reeds bewoonde site opgetrokken werd is het typevoorbeeld van een villa met gevelgalerij. In de tweede eeuw werden achter het huis baden bijgebouwd, die vaak herbouwd werden in tegenstelling tot de woonvertrek- ken die vrijwel niet veranderd werden. Tijdens een latere période werd voor de galerij een soort drogerij ingericht. In gebouw B vond men zo nog een installatie. De site is blijkbaar in gebruik geweest tot het einde van de IVe of het begin van de Ve eeuw, en werd dan definitief verlaten.
Central tarsal bone fracture in a cat
Authors: Cinti Filippo , Penazzi Claudio , Carusi Umberto , Vezzoni Luca , Vezzoni Aldo , Pisani Guido
Published: February 2016

Fracture of the central tarsal bone is an uncommon injury in dogs and occurs predominantly in racing Greyhounds. To the authors’ knowledge, this type of fracture has not been described previously in cats. This case report describes a five-year-old Domestic Shorthair cat referred to the Centro Veterinario Luni Mare because of lameness, swelling and signs of pain in the right hindlimb caused by trauma. Clinical examination and diagnostic imaging revealed a right central tarsal bone fracture. Open reduction and internal fixation with a 2.0 mm position screw and two 0.8 mm Kirschner wires were carried out. The last follow-up examination three years postoperatively found the cat in good health with normal range of motion and function, and no signs of lameness in the right hindlimb.
Optogenetic Stimulation of Neural Grafts Enhances Neurotransmission and Downregulates the Inflammatory Response in Experimental Stroke Model
Authors: Daadi Marcel M. , Klausner Jill Q. , Bajar Bryce , Goshen Inbal , Lee-Messer Christopher , Lee Soo Yeun , Winge Mårten C. G. , Ramakrishnan Charu , Lo Maisie , Sun Guohua , Deisseroth Karl , Steinberg Gary K.
Published: February 2016

Compelling evidence suggests that transplantation of neural stem cells (NSCs) from multiple sources ameliorates motor deficits after stroke. However, it is currently unknown to what extent the electrophysiological activity of grafted NSC progeny participates in the improvement of motor deficits and whether excitatory phenotypes of the grafted cells are beneficial or deleterious to sensorimotor performances. To address this question, we used optogenetic tools to drive the excitatory outputs of the grafted NSCs and assess the impact on local circuitry and sensorimotor performance. We genetically engineered NSCs to express the Channelrhodopsin-2 (ChR2), a light-gated cation channel that evokes neuronal depolarization and initiation of action potentials with precise temporal control to light stimulation. To test the function of these cells in a stroke model, rats were subjected to an ischemic stroke and grafted with ChR2-NSCs. The grafted NSCs identified with a human-specific nuclear marker survived in the peri-infarct tissue and coexpressed the ChR2 transgene with the neuronal markers TuJ1 and NeuN. Gene expression analysis in stimulated versus vehicle-treated animals showed a differential upregulation of transcripts involved in neurotransmission, neuronal differentiation, regeneration, axonal guidance, and synaptic plasticity. Interestingly, genes involved in the inflammatory response were significantly downregulated. Behavioral analysis demonstrated that chronic optogenetic stimulation of the ChR2-NSCs enhanced forelimb use on the stroke-affected side and motor activity in an open field test. Together these data suggest that excitatory stimulation of grafted NSCs elicits beneficial effects in experimental stroke model through cell replacement and non-cell replacement, anti-inflammatory/neurotrophic effects.
Effect of an Epineurial-Like Biohybrid Nerve Conduit on Nerve Regeneration
Authors: Hsieh Shu-Chih , Chang Chen-Jung , Cheng Wen-Tung , Tseng Ting-Chen , Hsu Shan-Hui
Published: February 2016

A novel approach of making a biomimetic nerve conduit was established by seeding adipose-derived adult stem cells (ADSCs) on the external wall of porous poly(D, L-lactic acid) (PLA) nerve conduits. The PLA conduits were fabricated using gas foaming salt and solvent–nonsolvent phase conversion. We examined the effect of two different porous structures (GS and GL) on ADSC growth and proliferation. The GS conduits had better structural stability, permeability, and porosity, as well as better cell viability at 4, 7, and 10 days. The epineuriallike tissue was grown from ADSC-seeded conduits cultured for 7 days in vitro and then implanted into 10-mm rat sciatic nerve defects for evaluation. The regeneration capacity and functional recovery were evaluated by histological staining, electrophysiology, walking track, and functional gait analysis after 6 weeks of implantation. Experimental data indicated that the autograft and ADSC-seeded GS conduits had better functional recovery than the blank conduits and ADSC-seeded GL conduits. The area of regenerated nerve and number of myelinated axons quantified based on the histology also indicated that the autograft and AGS groups performed better than the other two groups. We suggested that ADSCs may interact with endogenous Schwann cells and release neurotrophic factors to promote peripheral nerve regeneration. The design of the conduit may be critical for producing a biohybrid nerve conduit and to provide an epineurial-like support.
Downregulation of Heme Oxygenase 1 (HO-1) Activity in Hematopoietic Cells Enhances Their Engraftment after Transplantation
Authors: Adamiak Mateusz , Moore Joseph B. , Zhao John , Abdelbaset-Ismail Ahmed , Grubczak Kamil , Rzeszotek Sylwia , Wysoczynski Marcin , Ratajczak Mariusz Z.
Published: February 2016

Heme oxygenase 1 (HO-1) is an inducible stress-response enzyme that not only catalyzes the degradation of heme (e.g., released from erythrocytes) but also has an important function in various physiological and pathophysiological states associated with cellular stress, such as ischemic/reperfusion injury. HO-1 has a well-documented anti-inflammatory potential, and HO-1 has been reported to have a negative effect on adhesion and migration of neutrophils in acute inflammation in a model of peritonitis. This finding is supported by our recent observation that hematopoietic stem progenitor cells (HSPCs) from HO-1 KO mice are easy mobilizers, since they respond better to peripheral blood chemotactic gradients than wild-type littermates. Based on these findings, we hypothesized that transient inhibition of HO-1 by nontoxic small-molecule inhibitors would enhance migration of HSPCs in response to bone marrow chemoattractants and thereby facilitate their homing. To directly address this issue, we generated several human hematopoietic cell lines in which HO-1 was upregulated or downregulated. We also exposed murine and human BM-derived cells to small-molecule activators and inhibitors of HO-1. Our results indicate that HO-1 is an inhibitor of hematopoietic cell migration in response to crucial BM homing chemoattractants such as stromal-derived factor 1 (SDF-1) and sphingosine-1-phosphate (S1P). Most importantly, our in vitro and in vivo animal experiments demonstrate for the first time that transiently inhibiting HO-1 activity in HSPCs by small-molecule inhibitors improves HSPC engraftment. We propose that this simple and inexpensive strategy could be employed in the clinical setting to improve engraftment of HSPCs, particularly in those situations in which the number of HSPCs available for transplant is limited (e.g., when transplanting umbilical cord blood).
Intrathecal Transplantation of Embryonic Stem Cell-Derived Spinal GABAergic Neural Precursor Cells Attenuates Neuropathic Pain in a Spinal Cord Injury Rat Model
Authors: Hwang Insik , Hahm Suk-Chan , Choi Kyung-Ah , Park Sung-Ho , Jeong Hyesun , Yea Ji-Hye , Kim Junesun , Hong Sunghoi
Published: February 2016

Neuropathic pain following spinal cord injury (SCI) is a devastating disease characterized by spontaneous pain such as hyperalgesia and allodynia. In this study, we investigated the therapeutic potential of ESC-derived spinal GABAergic neurons to treat neuropathic pain in a SCI rat model. Mouse embryonic stem cell–derived neural precursor cells (mESC-NPCs) were cultured in media supplemented with sonic hedgehog (SHH) and retinoic acid (RA) and efficiently differentiated into GABAergic neurons. Interestingly, low doses of SHH and RA induced MGE-like progenitors, which expressed low levels of DARPP32 and Nkx2.1 and high levels of Irx3 and Pax6. These cells subsequently generated the majority of the DARPP32- GABAergic neurons after in vitro differentiation. The spinal mESC-NPCs were intrathecally transplanted into the lesion area of the spinal cord around T10–T11 at 21 days after SCI. The engrafted spinal GABAergic neurons remarkably increased both the paw withdrawal threshold (PWT) below the level of the lesion and the vocalization threshold (VT) to the level of the lesion (T12, T11, and T10 vertebrae), which indicates attenuation of chronic neuropathic pain by the spinal GABAergic neurons. The transplanted cells were positive for GABA antibody staining in the injured region, and cells migrated to the injured spinal site and survived for more than 7 weeks in L4–L5. The mESC-NPC-derived spinal GABAergic neurons dramatically attenuated the chronic neuropathic pain following SCI, suggesting that the spinal GABAergic mESC-NPCs cultured with low doses of SHH and RA could be alternative cell sources for treatment of SCI neuropathic pain by stem cell-based therapies.
Outcome of Burns Treated with Autologous Cultured Proliferating Epidermal Cells: A Prospective Randomized Multicenter Intrapatient Comparative Trial
Authors: Gardien Kim L. M. , Marck Roos E. , Bloemen Monica C. T. , Waaijman Taco , Gibbs Sue , Ulrich Magda M. W. , Middelkoop Esther
Published: February 2016

Standard treatment for large burns is transplantation with meshed split skin autografts (SSGs). A disadvantage of this treatment is that healing is accompanied by scar formation. Application of autologous epidermal cells (keratinocytes and melanocytes) may be a suitable therapeutic alternative, since this may enhance wound closure and improve scar quality. A prospective, multicenter randomized clinical trial was performed in 40 adult patients with acute full thickness burns. On two comparable wound areas, conventional treatment with SSGs was compared to an experimental treatment consisting of SSGs in combination with cultured autologous epidermal cells (ECs) seeded in a collagen carrier. The primary outcome measure was wound closure after 5–7 days. Secondary outcomes were safety aspects and scar quality measured by graft take, scar score (POSAS), skin colorimeter (DermaSpectrometer®) and elasticity (Cutometer®). Wound epithelialization after 5–7 days was significantly better for the experimental treatment (71%) compared to the standard treatment (67%) ( p = 0.034, Wilcoxon), whereas the take rates of the grafts were similar. No related adverse events were recorded. Scar quality was evaluated at 3 ( n = 33) and 12 ( n = 28) months. The POSAS of the observer after 3 and 12 months and of the patient after 12 months were significantly better for the experimental area. Improvements between 12% and 23% ( p ≤ 0.010, Wilcoxon) were detected for redness, pigmentation, thickness, relief, and pliability. Melanin index at 3 and 12 months and erythema index at 12 months were closer to normal skin for the experimental treatment than for conventional treatment ( p ≤ 0.025 paired samples t-test). Skin elasticity showed significantly higher elasticity ( p = 0.030) in the experimental area at 3 months follow-up. We showed a safe application and significant improvements of wound healing and scar quality in burn patients after treatment with ECs versus SSGs only. The relevance of cultured autologous cells in treatment of extensive burns is supported by our current findings.
Indiscernible Benefit of Double-Unit Umbilical Cord Blood Transplantation in Children: A Single-Center Experience from Hong Kong
Authors: Tsang Kam Sze , Leung Alex Wing Kwan , Lee Vincent , Cheng Frankie Wai Tsoi , Shing Matthew Ming Kong , Pong Henry Nga Hin , Leung Ting Fan , Yuen Patrick Man Pan , Li Chi Kong
Published: February 2016

Double-unit umbilical cord blood (DU-UCB) may extend the use of UCB transplantation and improve clinical outcomes. Data in the literature show that single-unit dominance happened in a vast majority of recipients, and the mechanism is unknown. We examined the clinical relevance and engraftment kinetics of DU-UCB transplant in 65 consecutive children who underwent unrelated single-unit ( n = 25) and double-unit ( n = 40) UCB transplantation for various hematological malignancies ( n = 45) and nonmalignant disorders ( n = 20). Our result showed no discernible benefit to children receiving double-unit transplant over those receiving single-unit transplant when the total nucleated cell (TNC) doses are ≥2.5 × 107/kg, in terms of the hastening of the engraftment of neutrophils and platelets, reduction of nonengraftment, disease recurrence, early mortality, and graft-versus-host disease, despite significantly higher numbers of TNCs in double units. Further analyses demonstrated that the phenomena were not associated with underlying disease, duration of UCB storage, postthaw viability, HLA disparity, ABO incompatibility, gender, or doses of TNCs, CD34+ cells, CD3+ cells, or colony-forming units. Engrafting units in DU-UCB transplants were notably associated with higher CD34+ cell dose. Chimerism studies demonstrated that single-unit dominance started before neutrophil engraftment in DU-UCB transplants. Data from the study suggested no advantage of infusing double-unit UCB, if an adequately dosed single-unit UCB is available. Successful prediction of the dominant graft would optimize algorithms of UCB selection and maximize the long-term engraftment of chosen units.
Determination of Islet Viability Using a Zinc-Specific Fluorescent Dye and a Semiautomated Assessment Method
Authors: Komatsu Hirotake , Omori Keiko , Parimi Mounika , Rawson Jeffrey , Kandeel Fouad , Mullen Yoko
Published: February 2016

Islet transplantation is an effective therapy that allows the achievement of insulin independence in patients with type 1 diabetes (T1D). To ensure successful transplantation, islet viability and function are of great importance. Viability assessments most often use fluorescein diacetate (FDA)/propidium iodide (PI) staining. However, results using this method often do not correlate well with graft function. Because FDA nonspecifically penetrates all cells present in the islet preparation, including islets and contaminating acinar cells, its use often complicates viability assessments of the overall cell population. Furthermore, the manual method for determining viability percentages is highly subjective. Shortcomings of the conventional islet viability assay can be potentially improved by staining cells with Newport Green (NG). NG, is a zinc-specific fluorescent dye that specifically reacts with zinc-rich β cells. Two kinds of NG dyes, NG-DCF and NG-PDX, are currently available. We examined the zinc specificity of these NG dyes and compared NG staining with traditional FDA staining to explore the potential of NG dyes to improve islet viability assessment. Of the two NGs tested, NG-DCF showed the higher specificity toward a β-cell line as well as human islets. NG-DCF accurately identified the islet area, even in low-purity islets, while neither FDA nor NG-PDX did. Although NG-DCF staining required a longer incubation time, the addition of poloxamer F127 and incubation at 37°C allowed viability assessment to take place within 30 min. Unlike FDA/PI staining, NG-DCF/PI staining allowed for islet-specific assessment. We also introduced a semiautomated measurement to determine NG-DCF/PI staining results, which enabled us to obtain objective and reproducible results. NG-DCF/PI staining is easy and reliable, and this method permits highly objective islet-specific viability assessments.