bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of the effects of autologous and allogeneic purified platelet-rich plasma on cartilage damage in a rabbit model of knee osteoarthritis
Authors: Wang Lingling , Zhao Luting , Shen Lianwei , Fang Qilin , Yang Zhenglei , Wang Rongrong , Wu Qing , Xie Yulei
Published: July 2022

Background

Purified platelet-rich plasma (P-PRP) is gradually being used in the treatment of osteoarthritis (OA), and its sources are mainly divided into autologous and allogeneic blood. However, it is unclear whether autologous PRP is more effective or allogeneic PRP is superior.

Objective

In this study, autologous and allogeneic P-PRP was injected at early stage of KOA in rabbits, and then the differences in the efficacy of the two P-PRPs against KOA were compared from several perspectives, including pathological histology and immunohistochemistry.

Method

Experimental rabbits were divided into normal group (n = 8), model group (n = 8), autologous P-PRP group (n = 8), and allogeneic P-PRP group (n = 8) using a random number table method. The normal and model groups did not receive any treatment, and the autologous P-PRP and allogeneic P-PRP groups received intra-articular injections of autologous and allogeneic P-PRP, respectively, to observe the changes in the gross specimens of the knee joints of the experimental rabbits in each group. The histopathological changes of chondrocytes were also observed by HE-stained sections of articular cartilage, and the expression of chondrocytes Bone morphogenetic protein-2 (BMP-2) and Sox9 were detected by immunohistochemistry.

Results

Compared with the allogeneic P-PRP group, the differences were statistically significant (P < 0.05) in the gross specimens and pathological histological findings in the autologous PRP group. Immunohistochemical results showed that the expression of BMP-2 and Sox9 was elevated in both the autologous P-PRP group and the allogeneic P-PRP group compared with the model group, and the expression of BMP-2 was higher in the autologous P-PRP group than in the allogeneic P-PRP group, with a statistically significant difference (P < 0.05), while there was no difference in the expression of Sox9 between the two groups (P > 0.05).

Conclusion

Intra-articular injection of autologous P-PRP activated the expression of BMP-2 and Sox9 in chondrocytes and effectively improved KOA cartilage repair and reduced bone redundancy and joint fluid formation, and its efficacy was superior to that of intra-articular injection of allogeneic P-PRP.
Toxidromes for Working Dogs
Authors: McMichael Maureen A. , Singletary Melissa , Akingbemi Benson T.
Published: July 2022

Terrorist attacks with biological and chemical warfare agents are increasing in frequency worldwide. Additionally, hazardous chemical accidents, illicit drug laboratories and intentional poisonings are potential sites for exposure to working dogs. Working dogs play a crucial role in law enforcement, military and search and rescue teams. Their intelligence, agility and strength make them ideal partners to be deployed to these natural disaster sites, terrorist attacks and industrial accidents. This, unfortunately, leads to increasing exposure to chemical and biological weapons and other hazardous substances. First responders have little to no training in emergency care of working dogs and veterinarians have very little training on recognition of the clinical signs of many of these agents. In order to ensure a rapid medical response at the scene first responders and veterinarians need a primer on these agents. Identifying a specific agent amidst the chaos of a mass casualty event is challenging. Toxidromes are a constellation of clinical and/or laboratory findings that allow for rapid identification of the clinical signs associated with a class of toxin and have been helpful in human medical triage. Focusing on a class of agents rather than on each individual toxin, allows for more expedient administration of antidotes and appropriate supportive care. This article reviews toxidromes for the most common chemical weapons with a special emphasis on clinical signs that are specific (and different) for canines as well as appropriate antidotes for working canines. To our knowledge, there are no publications describing toxidromes for working dogs.
Proteome Informatics in Tibetan Sheep (Ovis aries) Testes Suggest the Crucial Proteins Related to Development and Functionality
Authors: Li Taotao , Wang Huihui , Luo Ruirui , An Xuejiao , Li Qiao , Su Manchun , Shi Huibin , Chen Haolin , Zhang Yong , Ma Youji
Published: July 2022

Testis has an indispensable function in male reproduction of domestic animals. Tibetan sheep (Ovis aries) is a locally adapted breed of sheep raised in the Qinghai-Tibet Plateau, with outsized roles in providing the livelihood for millions of residents. Nevertheless, less is known on how protein expression and their functional roles in developmental testes of such breed limit their use in breeding efforts. In this study, we obtained comprehensive protein profiles from testes of Tibetan sheep at three developmental stages (including pre-puberty, post-puberty, and adulthood) using data-independent acquisition-based proteomic strategy to quantitatively identify the differentially abundant proteins (DAPs) associated with testicular development and function and to unravel the molecular basis of spermatogenesis. A total of 6,221 proteins were differentially expressed in an age-dependent manner. The reliability of the gene expression abundance was corroborated by quantitative PCR and targeted parallel reaction monitoring. These DAPs were significantly enriched to biological processes concerning spermatid development and sperm deformation, mitosis, glycolytic process, cell-cell/extracellular matrix (ECM) junctions, cell proliferation, apoptosis, and migration and to the pathways including, developmental process and sexual reproduction-related (such as VEGF, estrogen, insulin, GnRH, Hippo, PI3K-Akt, mTOR, MAPK, and AMPK), and testicular cell events-related pathways (such as tight/gap/adherens junctions, ECM-receptor interaction, regulation of actin cytoskeleton, glycolysis, cell cycle, and meiosis). Based on these bioinformatics analysis, we constructed four protein–protein interaction network, among which the proteins are involved in mitosis, meiosis, spermiogenesis, and testicular microenvironment, respectively. Altogether, these bioinformatics-based sequencing results suggest that many protein-coding genes were expressed in a development-dependent manner in Tibetan sheep testes to contribute to the testicular cell development and their surrounding microenvironment remodeling at various stages of spermatogenesis. These findings have important implications for further understanding of the mechanisms underlying spermatogenesis in sheep and even other plateau-adapted animals.
Trust Deficit in Surgical Systems in an Urban Slum in India Under Universal Health Coverage: A Mixed Method Study
Authors: Vora Kranti , Saiyed Shahin , Mavalankar Dileep , Baines Lyndsay S. , Jindal Rahul M.
Published: July 2022

Objectives: We carried out a mixed method study to understand why patients did not avail of surgical care in an urban slum in India.

Methods: In our earlier study, we found that out of 10,330 people, 3.46% needed surgery; 42% did not avail of surgery (unmet needs). We conducted a follow-up study to understand reasons for not availing surgery, 141 in met needs, 91 in unmet needs. We administered 2 instruments, 16 in-depth interviews and 1 focused group discussion.

Results: Responses from the 2 groups for “the Socio-culturally Competent Trust in Physician Scale for a Developing Country Setting” scale did not have significant difference except for, prescription of medicines, patients with unmet needs were less likely to agree (p = 0.076). Results between 2 groups regarding “Patient perceptions of quality” did not show significant difference except for doctors answering questions where a higher proportion of unmet need group agreed (p = 0.064). Similar observations were made in the in depth interviews and focus group.

Conclusion: There is a need for understanding trust issues with health service delivery related to surgical care for marginalized populations.
Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods
Authors: Liang Zi-Qian , He Rong-Quan , Luo Jia-Yuan , Huang Zhi-Guang , Li Jie , Zhong Lu-Yang , Chen Jun-Hong , Huang Su-Ning , Shi Lin , Wei Kang-Lai , Zeng Jiang-Hui , Zeng Jing-Jing , Chen Gang
Published: July 2022

Introduction: We aimed to explore the abnormal expression of dual-specificity protein phosphatase 1 (DUSP1) and its latent molecular mechanisms in ovarian carcinoma (OVCA).

Materials and Methods: Two clinical cohorts collected from two different hospitals were used to evaluate the expression of DUSP1 protein in OVCA tissues. RNA-sequencing and microarray datasets were utilised to verify DUSP1 expression at mRNA levels in both OVCA tissues and in the peripheral blood of OVCA patients. Furthermore, an integrated calculation was performed to pool the standard mean difference (SMD) from each cohort in order to comprehensively assess the expression of DUSP1 in OVCA. Furthermore, we examined the relationship among DUSP1, tumour microenvironment (TME), and chemotherapy resistance in OVCA. Moreover, we used pathway enrichment analysis to explore the underlying mechanisms of DUSP1 in OVCA.

Results: A pooled SMD of −1.19 (95% CI [−2.00, −0.38], p = 0.004) with 1,240 samples revealed that DUSP1 was downregulated in OVCA at both mRNA and protein levels. The area under the receiver operating characteristic curve of 0.9235 indicated the downregulated DUSP1 in peripheral blood may have a non-invasive diagnostic value in OVCA. Through six algorithms, we identified that DUSP1 may related to tumour-infiltrating T cells and cancer associated fibroblasts (CAFs) in OVCA. Pathway enrichment demonstrated that DUSP1 might participate in the mitogen-activated protein kinase (MAPK) signalling pathway. Furthermore, DUSP1 may have relations with chemotherapy resistance, and a favourable combining affinity was observed in the paclitaxel-DUSP1 docking model.

Conclusion: DUSP1 was downregulated in OVCA, and this decreasing trend may affect the infiltration of CAFs. Finally, DUSP1 may have a targeting relation with paclitaxel and participate in MAPK signaling pathways.
Altrenogest Supplementation during Early Pregnancy Improves Reproductive Outcome in Pigs
Authors: Muro Bruno Bracco Donatelli , Oliveira Ana Clara Rodrigues , Carnevale Rafaella Fernandes , Leal Diego Feitosa , Monteiro Matheus Saliba , Poor André Pegoraro , Pereira Francisco Alves , de Souza Leury Jesus , Ferreira Juliana Bonin , Almond Glen William , Garbossa Cesar Augusto Pospissil
Published: July 2022

Progesterone plays an important role in initial conceptus development and in a successful pregnancy, but results related to progesterone or its analogues (altrenogest) supplementation in early pregnancy of pigs are conflicting. The present study evaluated the effects of altrenogest supplementation in sows during days 6 and 12 of pregnancy on reproductive performance. On day 6 of pregnancy, 301 females were allocated at random to one of the following treatments: CON (Control: non-supplemented females, n = 163) or ALT (females daily supplemented with 20 mg of altrenogest, orally, from day 6 to 12 of pregnancy, n = 138). Ovulation was considered as occurred at 48 h after the first estrus detection to standardize the first day of pregnancy. The supplementation increased the number of total piglets born (ALT: 17.3 ± 0.4; CON: 16.6 ± 0.4), piglets born alive (ALT: 15.6 ± 0.4; CON: 14.8 ± 0.3), and placenta weight (ALT: 4.2 ± 0.1; CON: 3.8 ± 0.1) and decreased the stillbirth rate (ALT: 5.9 ± 0.6; CON: 7.6 ± 0.6) and the number of piglets born weighing less than 800 g (ALT: 6.6 ± 0.6; CON: 8.0 ± 0.6), without impairment on farrowing rate. These results demonstrated that altrenogest supplementation on swine females between days 6 and 12 of pregnancy may be used to improve reproductive performance.
Causes of Sow Mortality and Risks to Post-Mortem Findings in a Brazilian Intensive Swine Production System
Authors: Monteiro Matheus Saliba , Matias Débora Novais , Poor André Pegoraro , Dutra Maurício Cabral , Moreno Luisa Zanolli , Parra Beatriz Martins , Silva Ana Paula Santos , Matajira Carlos Emílio Cabrera , de Moura Gomes Vasco Túlio , Barbosa Mikaela Renata Funada , Sato Maria Inês Zanoli , Moreno Andrea Micke
Published: July 2022

The present study was conducted to investigate the risk factors for post-mortem findings and causes of sow mortality. A post-mortem examination and microbiological investigation were conducted on 123 sows from a breeding herd with 15,000 dams. The mortality of spontaneous death in sows occurred mostly in the peripartum period (53%; p < 0.05). The spontaneous deaths were associated with heart failures, hemorrhagic and perforating gastric ulcers, and liver torsion, while in the euthanized sows, the post-mortem findings were associated with locomotor disorders. A higher body condition score (BCS ≥ 3.5) increased (p < 0.05) heart failure on the post-mortem examination. The excessive use of manual obstetric interventions increased sow deaths resulting from cervix/uterus ruptures and increased the odds of death (p < 0.05) due to metritis. Sow mortality had a multifactorial etiology. Infections were polymicrobial. The main microbial agents identified from a septic lesion in locomotor, genitourinary, and respiratory systems were Trueperella pyogenes, Escherichia coli, and Actinobacillus pleuropneumoniae, respectively. In conclusion, sow mortality involved multiple risk factors and several bacterial agents. These results indicate that better management practices can reduce sow mortality in swine production and increase sow welfare.
Epidemiological and Molecular Study of Cryptosporidium in Preweaned Calves in Kuwait
Authors: Majeed Qais A. H. , AlAzemi Maha S. , Al-Sayegh Mohammed T. , Abdou Nadra-Elwgoud M. I.
Published: July 2022

Cryptosporidium is a worldwide enteric protozoan parasite that causes gastrointestinal infection in animals, including humans. The most notable species is Cryptosporidium parvum because of its zoonotic importance; it is also the leading cause of cryptosporidiosis in preweaned calves. A cross-sectional study was conducted to determine the prevalence of Cryptosporidium infection, investigate the potential risk factors, and use molecular diagnosis to identify the predominant Cryptosporidium spp. in preweaned calves in Kuwait. Of 175 preweaned calves, Cryptosporidium antigens were detected in 58 (33.1%) using rapid lateral immunochromatography assay (IC). Calves less than one month of age (OR = 4.32, p = 0.0001) and poor hygiene (OR = 2.85, p = 0.0075) were identified as significant risk factors associated with Cryptosporidium infection. Molecular identification revealed that C. parvum (62.8%) was the dominant species infecting preweaned calves in Kuwait. In contrast, C. bovis and C. andersoni were recorded at 5.7% and 2.9%, respectively. All C. parvum gp60 nucleotide sequences were subtype IIaA15G2R1. Calves could be a source of C. parvum infection due to the similarity of the subtypes recorded previously in Kuwaiti children and preweaned calves in this study. Therefore, more research is needed to understand the Cryptosporidium transmission cycle in Kuwait.
Comparison of Nutritive Values of Tropical Pasture Species Grown in Different Environments, and Implications for Livestock Methane Production: A Meta-Analysis
Authors: Jayasinghe Priyanath , Ramilan Thiagarajah , Donaghy Daniel J. , Pembleton Keith G. , Barber David G.
Published: July 2022

The demand for dairy products is ever increasing across the world. The livestock sector is a significant source of greenhouse gas (GHG) emissions globally. The availability of high-quality pasture is a key requirement to increase the productivity of dairy cows as well as manage enteric methane emissions. Warm-season perennial grasses are the dominant forages in tropical and subtropical regions, and thus exploring their nutritive characteristics is imperative in the effort to improve dairy productivity. Therefore, we have collated a database containing a total of 4750 records, with 1277 measurements of nutritive values representing 56 tropical pasture species and hybrid cultivars grown in 26 different locations in 16 countries; this was done in order to compare the nutritive values and GHG production across different forage species, climatic zones, and defoliation management regimes. Average edaphoclimatic (with minimum and maximum values) conditions for tropical pasture species growing environments were characterized as 22.5 °C temperature (range 17.5–29.30 °C), 1253.9 mm rainfall (range 104.5–3390.0 mm), 582.6 m elevation (range 15–2393 m), and a soil pH of 5.6 (range 4.6–7.0). The data revealed spatial variability in nutritive metrics across bioclimatic zones and between and within species. The ranges of these nutrients were as follows: neutral detergent fibre (NDF) 50.9–79.8%, acid detergent fibre (ADF) 24.7–57.4%, crude protein (CP) 2.1–21.1%, dry matter (DM) digestibility 30.2–70.1%, metabolisable energy (ME)3.4–9.7 MJ kg−1 DM, with methane (CH4) production at 132.9–133.3 g animal−1 day−1. The arid/dry zone recorded the highest DM yield, with decreased CP and high fibre components and minerals. Furthermore, the data revealed that climate, defoliation frequency and intensity, in addition to their interactions, have a significant effect on tropical pasture nutritive values and CH4 production. Overall, hybrid and newer tropical cultivars performed well across different climates, with small variations in herbage quality. The current study revealed important factors that affect pasture nutritive values and CH4 emissions, with the potential for improving tropical forage through the selection and management of pasture species.
Impacts of Increasing Additions of Choline Chloride on Growth Performance and Carcass Characteristics of Broiler Chickens Reared to 66 Days of Age
Authors: Gregg Caroline R. , Tejeda Oscar J. , Spencer Lindsey F. , Calderon Allan J. , Bourassa Dianna V. , Starkey Jessica D. , Starkey Charles W.
Published: July 2022

The most recent research cited by the NRC Nutrition Requirements of Poultry to establish choline recommendations was published in 1987, so choline guidelines for modern broilers are outdated and may be insufficient to optimize growth. The objective was to determine the effect of additional dietary choline chloride supplementation on growth performance and carcass characteristics of modern broilers reared for 66 days. As-hatched Ross 708 × Yield Plus broiler chicks (n = 2160; 30 birds per pen) were randomly allotted to one of six experimental corn and soybean meal-based diets formulated to contain an additional 0, 400, 800, 1200, 1600, or 2000 mg of choline chloride above the choline content of the basal diet ingredients. Diets were fed in four phases, and birds were processed at day 66 of age. Growth performance and breast myopathy incidence was not impacted by added choline. While there were differences in breast, wing, thigh, and drum yields, the effects of added choline were not linear. Supplemental choline chloride was not beneficial for growth performance but did impact the carcass characteristics of modern, large frame broilers reared for 66 days.
In Silico Screening and Development of Microsatellite Markers for Genetic Analysis in Perca fluviatilis
Authors: Xu Pu , Lu Cuiyun , Sun Zhipeng , Kuang Youyi , Cao Dingchen , Huo Tangbin , Li Chao , Jin Hongyu , Zheng Xianhu
Published: July 2022

Perca fluviatilis is an economically important species of freshwater fish. To understand the genetic structure of P. fluviatilis in China, 268 samples were collected from Wulungu Lake (WL), Jili Lake (JL), the Wulungu River (WR), and the Kalaeerqisi River (KR). These samples were then analyzed using microsatellite markers. A total of 98,425 microsatellite markers were developed based on the genomic data, and 29 polymorphic microsatellite markers were selected to analyze genetic diversity in this study. The number of alleles (Na) and observed heterozygosity (Ho) per population ranged from 4.621 (KR) to 11.172 (WL) and from 0.510 (KR) to 0.716 (JL), respectively. The results of the polymorphic information content (PIC) showed that the WL, JL, and WR populations were highly polymorphic (PIC≥ 0.5) and that the KR population was moderately polymorphic (0.25 ≤ PIC < 0.5). The genetic differentiation coefficient (Fst) among the four P. fluviatilis populations was 0.074, indicating moderate genetic differentiation among the populations in Xinjiang. The reason for the significant difference between the rivers and lakes could be the presence of a dam blocking the flow of P. fluviatilis. The development of microsatellite markers provides support for population genetics in the future. The evaluation of the genetic structure of P. fluviatilis in Xinjiang provides a reference for the reproduction and conservation of P. fluviatilis.
Investigating the Antituberculosis Activity of Selected Commercial Essential Oils and Identification of Active Constituents Using a Biochemometrics Approach and In Silico Modeling
Authors: Boussamba-Digombou Katyna J. , Sandasi Maxleene , Kamatou Guy P. , van Vuuren Sandy , Sawicki Rafal , Fakhar Zeynab , Viljoen Alvaro M.
Published: July 2022

Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis which has become prevalent due to the emergence of resistant M. tuberculosis strains. The use of essential oils (EOs) as potential anti-infective agents to treat microbial infections, including TB, offers promise due to their long historical use and low adverse effects. The current study aimed to investigate the in vitro anti-TB activity of 85 commercial EOs, and identify compounds responsible for the activity, using a biochemometrics approach. A microdilution assay was used to determine the antimycobacterial activity of the EOs towards some non-pathogenic Mycobacterium strains. In parallel, an Alamar blue assay was used to investigate antimycobacterial activity towards the pathogenic M. tuberculosis strain. Chemical profiling of the EOs was performed using gas chromatography-mass spectrometry (GC-MS) analysis. Biochemometrics filtered out putative biomarkers using orthogonal projections to latent structures discriminant analysis (OPLS-DA). In silico modeling was performed to identify potential therapeutic targets of the active biomarkers. Broad-spectrum antimycobacterial activity was observed for Cinnamomum zeylanicum (bark) (MICs = 1.00, 0.50, 0.25 and 0.008 mg/mL) and Levisticum officinale (MICs = 0.50, 0.5, 0.5 and 0.004 mg/mL) towards M. smegmatis, M. fortuitum, M. gordonae and M. tuberculosis, respectively. Biochemometrics predicted cinnamaldehyde, thymol and eugenol as putative biomarkers. Molecular docking demonstrated that cinnamaldehyde could serve as a scaffold for developing a novel class of antimicrobial compounds by targeting FtsZ and PknB from M. tuberculosis.
Deep and Hybrid Learning Technique for Early Detection of Tuberculosis Based on X-ray Images Using Feature Fusion
Authors: Fati Suliman Mohamed , Senan Ebrahim Mohammed , ElHakim Narmine
Published: July 2022

Tuberculosis (TB) is a fatal disease in developing countries, with the infection spreading through direct contact or the air. Despite its seriousness, the early detection of tuberculosis by means of reliable techniques can save the patients’ lives. A chest X-ray is a recommended screening technique for locating pulmonary abnormalities. However, analyzing the X-ray images to detect abnormalities requires highly experienced radiologists. Therefore, artificial intelligence techniques come into play to help radiologists to perform an accurate diagnosis at the early stages of TB disease. Hence, this study focuses on applying two AI techniques, CNN and ANN. Furthermore, this study proposes two different approaches with two systems each to diagnose tuberculosis from two datasets. The first approach hybridizes two CNN models, which are Res-Net-50 and GoogLeNet techniques. Prior to the classification stage, the approach applies the principal component analysis (PCA) algorithm to reduce the features’ dimensionality, aiming to extract the deep features. Then, the SVM algorithm is used for classifying features with high accuracy. This hybrid approach achieved superior results in diagnosing tuberculosis based on X-ray images from both datasets. In contrast, the second approach applies artificial neural networks (ANN) based on the fused features extracted by ResNet-50 and GoogleNet models and combines them with the features extracted by the gray level co-occurrence matrix (GLCM), discrete wavelet transform (DWT) and local binary pattern (LBP) algorithms. ANN achieved superior results for the two tuberculosis datasets. When using the first dataset, the ANN, with ResNet-50, GLCM, DWT and LBP features, achieved an accuracy of 99.2%, a sensitivity of 99.23%, a specificity of 99.41%, and an AUC of 99.78%. Meanwhile, with the second dataset, ANN, with the features of ResNet-50, GLCM, DWT and LBP, reached an accuracy of 99.8%, a sensitivity of 99.54%, a specificity of 99.68%, and an AUC of 99.82%. Thus, the proposed methods help doctors and radiologists to diagnose tuberculosis early and increase chances of survival.
Skin Temperature: The Impact of Perfusion, Epidermis Thickness, and Skin Wetness
Authors: Saiko Gennadi
Published: July 2022

This work aimed to elucidate the primary factors which affect skin temperature. A simple thermophysical model of the skin, which accounts for radiative, convective, and evaporative heat losses, has been developed to address it. The model is based on the skin’s morphology and consists of passive (nonviable tissue) and active (viable tissue) layers. The bioheat equation was solved for these layers using realistic assumptions. It was found that other than the ambient temperature, blood perfusion and epidermis thickness are the primary factors responsible for the skin temperature variations. The main temperature drop in the skin is attributed to the cooling of the blood in the venous plexus. The temperature drop in the epidermis is on the scale of 0.1 °C for the normal epidermis but can be 1.5–2 °C or higher in calluses. Thus, local skin temperature variations can indicate the epidermis thickness variations, particularly in callus-prone areas. The effects of relative air humidity and skin wetness on skin temperature were also quantified. The presence of free moisture on the skin (e.g., wet wound) significantly increases the heat transfer, resulting in a skin temperature drop, which can be on the scale of several degrees Celsius. The relative air humidity significantly contributes (by slowing heat dissipation) only in the case of evaporative heat loss from wet skin. Therefore, wet skin is undesirable and should be avoided during a thermographic assessment.
The Influence of the Discharge Port Structure on the Infrared Characteristics of Underwater Vehicle Thermal Jets
Authors: Gao Chengzhe , Du Yongcheng , Yang Li
Published: July 2022

The circulating cooling water of underwater vehicle power systems is discharged through the discharge port and is mixed with the environmental water to form a thermal jet, which diffuses, floats up in the environmental water and displays infrared characteristics on the surface of the water body. In order to explore the influence of the discharge port structure on the infrared characteristics of underwater vehicle thermal jets, this paper adopts the methods of simulation analysis and experimental verification, and it establishes an underwater vehicle motion model based on the CFD computing software platform to design elliptic discharge port structures with different radius ratios and to compare their different thermal jet infrared characteristics. The influence of the oval discharge port radius ratio on the infrared characteristics of thermal jets is verified by the reduced-scale water tank experiment, and the authenticity of the simulation calculation method and design parameters is verified. On the basis of oval discharge ports, the number and distribution position of discharge ports are further designed to suppress the infrared characteristics of thermal jets and to improve the thermal stealth performance of underwater vehicles. According to the simulation calculation and experimental results, under the condition of the same discharge flow, as the radius ratio becomes smaller, the mixing heat transfer effect of the thermal jet becomes better, and the infrared characteristics become less obvious. Moreover, increasing the number of discharge ports and adopting the symmetrical arrangement of discharge ports can further strengthen the temperature attenuation of thermal jets and reduce the surface maximum temperature.
Conceptual Design Study Based on Reliability Assessment of Secondary Energy Conversion Mechanical System in Movable Object Type Wave Power Generator
Authors: Kim Tae-Wook , Park Ji-Yong , Oh Jae-Won , Kim Kyong-Hwan , Lee Jung-Hee , Kim Hyung-Woo
Published: July 2022

Movable object type wave power generators produce energy through the process of primary energy conversion, which converts the potential and kinetic energy of the waves into mechanical energy, secondary energy conversion that converts it into energy for generator operation using a mechanical system or hydraulic system, and final energy conversion, the last step in power generation. The secondary energy conversion system that transmits and amplifies energy according to the primary energy conversion needs to secure durability while power generation performance varies greatly depending on how the secondary energy conversion system is built. As a result, reliability assessment of systems based on system engineering are a very important issue. Therefore, in this study, for the conceptual design based on reliability assessment of the secondary energy conversion system, the system concept was established using the integrated computer-aided manufacturing (ICAM) definition for function modeling (IDEF0), a system analysis method, while necessary equipment and process flow diagrams (PFD) were derived. In addition, the database (DB) and formula of the secondary energy conversion system were constructed, and reliability assessment algorithms and programs were developed. Finally, the PFD and reliability assessment program were verified by applying them to a representative movable object type wave power generator.
Human Activity Recognition Based on Non-Contact Radar Data and Improved PCA Method
Authors: Zhao Yixin , Zhou Haiyang , Lu Sichao , Liu Yanzhong , An Xiang , Liu Qiang
Published: July 2022

Human activity recognition (HAR) can effectively improve the safety of the elderly at home. However, non-contact millimeter-wave radar data on the activities of the elderly is often challenging to collect, making it difficult to effectively improve the accuracy of neural networks for HAR. We addressed this problem by proposing a method that combines the improved principal component analysis (PCA) and the improved VGG16 model (a pre-trained 16-layer neural network model) to enhance the accuracy of HAR under small-scale datasets. This method used the improved PCA to enhance features of the extracted components and reduce the dimensionality of the data. The VGG16 model was improved by deleting the complex Fully-Connected layers and adding a Dropout layer between them to prevent the loss of useful information. The experimental results show that the accuracy of our proposed method on HAR is 96.34%, which is 4.27% higher after improvement, and the training time of each round is 10.88 s, which is 12.8% shorter than before.
Effects of Cowl Lip Angle on Starting Performances for a Mixed-Compression Two-Dimensional Axisymmetric Hypersonic Inlet
Authors: Zhou Yunfan , Xu Shangcheng , Wang Yi
Published: July 2022

The third quasi-uniform B-spline was adopted to design the cowl profiles with various cowl lip angles. Starting performances were simulated by the numerical method and the results showed that the starting processes for different cowls can be classified into two types. In the first type, the inlet starts when the opened large-scale separation zone is swallowed; in the second type, the opened separation zone enters the internal contraction region to form a closed separation zone, which is then swallowed to enable the start. In addition, inlets with small and large cowl lip angles exhibit the first and second types of the starting process, respectively. Moreover, there is an optimal cowl lip angle to achieve the best starting performance. For the inlet investigated herein, the starting Mach number is the lowest when the cowl lip angle is 9°. In addition, as the internal contraction ratio increases, the optimal cowl lip angle gradually decreases and the starting performance deteriorates.
Trends in Summer-Time Tropospheric Ozone during COVID-19 Lockdown in Indian Cities Might Forecast a Higher Future Risk
Authors: Das Sujit , Sarkar Abhijit , Mina Usha , Nandy Senjuti , Saadat Md Najmus , Agrawal Ganesh Kumar , Rakwal Randeep
Published: July 2022

High concentrations of tropospheric ozone (O3) is a serious concern in India. The generation and atmospheric dynamics of this trace gas depend on the availability of its precursors and meteorological variables. Like other parts of the world, the COVID-19 imposed lockdown and restrictions on major anthropogenic activities executed a positive impact on the ambient air quality with reduced primary pollutants/precursors load. In spite of this, several reports pointed towards a higher O3 in major Indian cities during the lockdown. The present study designed with 30 pan-Indian mega-, class I-, and class II-cities revealed critical and contrasting aspects of the geographical location, source, precursor, and meteorological variable dependency of the spatial and temporal O3 formation. This unexpected O3 increase in the major cities might forecast the probable future risks for the National Air Quality policies, especially O3 pollution management, in the Indian sub-continent. The results also pointed towards the severity of the north Indian air quality, followed by the western and eastern parts. We believe these results will definitely pave the way for researchers and policy-makers for predicting/framing regional and/or national O3 management strategies in the future.
Epigenetic Reprogramming of the Inflammatory Response in Obesity and Type 2 Diabetes
Authors: Zatterale Federica , Raciti Gregory Alexander , Prevenzano Immacolata , Leone Alessia , Campitelli Michele , De Rosa Veronica , Beguinot Francesco , Parrillo Luca
Published: July 2022

For the past several decades, the prevalence of obesity and type 2 diabetes (T2D) has continued to rise on a global level. The risk contributing to this pandemic implicates both genetic and environmental factors, which are functionally integrated by epigenetic mechanisms. While these conditions are accompanied by major abnormalities in fuel metabolism, evidence indicates that altered immune cell functions also play an important role in shaping of obesity and T2D phenotypes. Interestingly, these events have been shown to be determined by epigenetic mechanisms. Consistently, recent epigenome-wide association studies have demonstrated that immune cells from obese and T2D individuals feature specific epigenetic profiles when compared to those from healthy subjects. In this work, we have reviewed recent literature reporting epigenetic changes affecting the immune cell phenotype and function in obesity and T2D. We will further discuss therapeutic strategies targeting epigenetic marks for treating obesity and T2D-associated inflammation.
Research on the Influencing Mechanism of Paradoxical Leadership on Unethical Pro-Supervisor Behavior
Authors: He Suchao , Yun Xiaoying
Published: July 2022

Paradoxical leadership is a leadership style that combines both employees’ individual needs and organizational requirements. The existing literature shows that paradoxical leadership has a positive influence on variables at the individual level, team level and organizational level. It is necessary to further explore the negative impact of paradoxical leadership on the individual level (such as employees’ unethical pro-supervisor behavior), the path of influence and situational conditions. Based on social exchange theory, this paper studied the influence of paradoxical leadership on employees’ unethical pro-supervisor behavior, and clarified the mediating role of supervisor–subordinate Guanxi and the moderating effect of follower mindfulness. We conducted an empirical analysis on the data of 356 employees collected in two phases, and found that paradoxical leadership exerts a significant positive effect on unethical pro-supervisor behavior; supervisor–subordinate Guanxi has a partial mediating effect on the relationship between paradoxical leadership and unethical pro-supervisor behavior; and follower mindfulness moderates the influence of paradoxical leadership on supervisor–subordinate Guanxi, and moderates the intermediation of supervisor–subordinate Guanxi on the main effect. This paper enriches the existing research on the mechanism of influence of paradoxical leadership and deepens our understanding of boundary conditions in relation to the role of paradoxical leadership.
The Classification and Mechanism of Microcrack Homogenization Research in Cement Concrete Based on X-ray CT
Authors: Wang Rui , Wu Hongjuan , Zhao Mohan , Liu Yu , Chen Chengqin
Published: July 2022

Old cement pavement directly overlaid with an asphalt layer produces many reflection cracks. Using microcrack homogenization technology to treat old cement pavement can effectively reduce the occurrence of reflection cracks. Micro-crack homogenization is relatively mature at the technical level; however, there is relatively little research on its mechanism. To evaluate the microcrack effect of old cement pavement, we conducted core sampling on a project road section after the appearance of microcracks. The core samples were sliced by X-ray tomography (X-ray CT). The mesostructure of the core samples was obtained. The core sample was further divided into microscopic cracks and macroscopic cracks according to the length and width of the crack. The development characteristics of cracks were subdivided into type I-III microcracks and type I-IV cracks. The core-drilling sample was divided into 5652 CT images. The statistical results showed that there were 3582 type-I microcracks, 3197 type-II microcracks, and 1835 type-III microcracks. Among the specimens, the minor proportion of microcracks was 32.87%, the most significant proportion was 100.00%, and the average ratio was 47.51%. Furthermore, the cracks development law and formation mechanism were analyzed based on CT images and cracking statistics. The results showed: (1) The microcrack homogenization process produced many microcracks in the test section and achieved a specific microcrack effect. (2) The cracks produced by the microcrack homogenization process tended to develop along the transition zone of the aggregate–cement mortar interface. The development of microcracks was mainly related to the aggregates’ shape and gradation, as well as the energy required to generate cracks. The research conclusions of this paper can be used as a theoretical basis for the optimization and improvement of the microcrack homogenization process.
Key Assessment Criteria for Organizational BIM Capabilities: A Cross-Regional Study
Authors: Rajabi Mohammad Sadra , Radzi Afiqah R. , Rezaeiashtiani Mohammad , Famili Alireza , Rashidi Mohammad Emad , Rahman Rahimi A.
Published: July 2022

Building information modeling (BIM) is an emerging process for managing the design, construction, operation, and maintenance of a facility. While BIM has developed in diverse aspects, the lack of organizational BIM capabilities remains a barrier to its implementation across the global architecture, engineering, and construction (AEC) industry. Accordingly, AEC organizations need to understand their organizational BIM capabilities and those of other organizations to realize the benefits of implementing BIM. This study examines the key criteria for assessing organizational BIM capabilities across two countries—Malaysia and Iran. For this purpose, the study compares the assessment criteria for BIM capabilities among the two countries based on the following elements: (1) criticality of the criteria; (2) degree of centrality of the criteria; and (3) underlying groups of the criteria. A systematic literature review of 26 articles and semi-structured interviews with BIM professionals provided nineteen criteria. A total of 121 and 126 BIM professionals evaluated the criticality of the criteria through a survey in Malaysia and Iran. The collected data were analyzed using the contextual disparities test (Mann–Whitney U test, Kruskal–Wallis H test, and rank agreement factor), network analysis, and exploratory factor analysis (EFA). The leading key criteria in both countries are “the company has the necessary infrastructure to implement BIM”, “the company has a good attitude towards new technology”, and “the company understands its expertise”. However, the subsequent key criteria differ between countries. Furthermore, while the level of agreement on the ranking of the criteria is at a neutral level, the Mann–Whitney U test indicates that the level of criticality significantly differs between countries for most criteria. There are also changes in the level of criticality of the criteria between countries. Finally, criteria with a high degree of centrality differ between countries. On the contrary, although the criteria slightly differ between countries, the overarching groups of the criteria are similar (i.e., the criteria are related to organizational BIM capabilities and organizational capabilities). Understanding these criteria can help researchers and industry practitioners develop the optimal tool for assessing organizational BIM capabilities for the local industry.
Effect of an Innovative Friction Damper on Seismic Responses of a Continuous Girder Bridge under Near-Fault Excitations
Authors: Guo Junjun , Li Huaifeng , Zhang Changyong , Chu Shihyu , Dang Xinzhi
Published: July 2022

Continuous girder bridges have been extensively constructed in China over the past 30 years, and these bridges tend to experience severe damage under ground motions with velocity pulses. In the current research, an innovative linear friction damper (LFD) is proposed to mitigate the seismic damages of continuous girder bridges subjected to near-fault ground motions. The OpenSees platform is adopted to establish the numerical model of a continuous girder bridge in the near-fault region. Sixteen ground motions with velocity pulses are selected from the PEER ground motion database. The wavelet method is used to extract the maximum velocity pulse from the two orthogonal components of a ground motion. The effects of the initial gap, the coefficient of friction, and the spring stiffness of LFD on the seismic responses of the bridge are investigated by the response surface method (RSM). The seismic responses of the bridge for the original system (Non-isolated), LFD system (Isolated-LFD), and lead rubber bearing (LRB) system (Isolated-LRB), such as force–displacement relationship, bearing displacement, and pier curvature, are obtained after conducting a series of nonlinear time history analyses. The numerical results reveal that this innovative device (LFD) can effectively control the relative displacements between the superstructure and substructure of the bridge. Meanwhile, the seismic responses of the piers can be significantly decreased compared with the non-isolated system.
Prospective Trial of Neutrophil/Lymphocyte Ratio and Other Blood Counts as Biomarkers of Survival among Patients with High-Grade Soft Tissue Sarcomas Treated with Pegylated Liposomal Doxorubicin and Ifosfamide
Authors: Skubitz Keith M. , Domingo-Musibay Evidio , Lindgren Bruce R. , Cheng Edward Y.
Published: July 2022

Several studies have reported an association between levels of circulating blood cells, in particular the neutrophil to lymphocyte ratio (absolute neutrophil count (ANC)/absolute lymphocyte count (ALC)) and outcomes in patients with cancer. In the current study, the association between lymphocyte, neutrophil, monocyte, and platelet counts and survival was examined in a prospective trial of preoperative pegylated-liposomal doxorubicin and ifosfamide for high-grade soft-tissue sarcomas. A statistically significant association between overall survival, but not progression free-survival, was observed with the ANC/ALC ratio at a cutoff value of ≥2 and a statistically significant trend using a cutoff of ≥5. Our results suggest that a balance between the lymphocyte count and the number of circulating myeloid cells that can suppress lymphocyte function may be predictive of survival in patients with soft-tissue sarcomas. Future research should therefore examine the role of lymphocyte-myeloid cell balance in sarcoma biology.
New Genetic Technologies in Diagnosis and Treatment of Cancer of Unknown Primary
Authors: Krawczyk Paweł , Jassem Jacek , Wojas-Krawczyk Kamila , Krzakowski Maciej , Dziadziuszko Rafał , Olszewski Włodzimierz
Published: July 2022

Cancer of unknown primary (CUP) represents a rare oncological and heterogeneous disease in which one or more metastases are present, but the location of the primary site is unknown. Pathological diagnosis, using immunohistochemistry, of such metastatic materials is challenging and frequently does not allow for determining the tissue of origin (ToO). The selection of systemic therapy in patients with CUP is usually based on empiric grounds, and the prognosis is generally unfavourable. New molecular techniques could identify the tissue of origin and be used to select systemic agnostic therapies in various malignancies with specific molecular abnormalities. Targetable driver mutations or gene rearrangements in cancer cells may be identified using various molecular assays, of which particularly valuable are next-generation sequencing techniques. These assays may identify tumour sources and allow personalized treatments. However, current guidelines for CUP management do not recommend routine testing of gene expression and epigenetic factors. This is mainly due to the insufficient evidence supporting the improvement of CUP’s prognosis by virtue of this approach. This review summarizes the advantages and disadvantages of new genetic techniques in CUP diagnostics and proposes updating the recommendations for CUP management.
Employing an Artificial Neural Network in Correlating a Hydrogen-Selective Catalytic Reduction Performance with Crystallite Sizes of a Biomass-Derived Bimetallic Catalyst
Authors: Yakub Ibrahim , Kueh Ahmad Beng Hong , Pineda De La O Edwin Andres , Rahman Md. Rezaur , Barawi Mohamad Hardyman , Abdullah Mohammad Omar , Amran Mugahed , Fediuk Roman , Vatin Nikolai Ivanovich
Published: July 2022

A predictive model correlating the properties of a catalyst with its performance would be beneficial for the development, from biomass waste, of new, carbon-supported and Earth-abundant metal oxide catalysts. In this work, the effects of copper and iron oxide crystallite size on the performance of the catalysts in reducing nitrogen oxides, in terms of nitrogen oxide conversion and nitrogen selectivity, are investigated. The catalysts are prepared via the incipient wetness method over activated carbon, derived from palm kernel shells. The surface morphology and particle size distribution are examined via field emission scanning electron microscopy, while crystallite size is determined using the wide-angle X-ray scattering and small-angle X-ray scattering methods. It is revealed that the copper-to-iron ratio affects the crystal phases and size distribution over the carbon support. Catalytic performance is then tested using a packed-bed reactor to investigate the nitrogen oxide conversion and nitrogen selectivity. Departing from chemical characterization, two predictive equations are developed via an artificial neural network technique—one for the prediction of NOx conversion and another for N2 selectivity. The model is highly applicable for 250–300 °C operating temperatures, while more data are required for a lower temperature range.