bluuebunny/crossref_metadata_2025 huggingface dataset:
Cytomegalovirus Viremia and Clinical Outcomes in Kenyan Children Diagnosed With Human Immunodeficiency Virus (HIV) in Hospital
Authors: Wamalwa Dalton , Njuguna Irene , Maleche-Obimbo Elizabeth , Begnel Emily , Chebet Daisy J , Onyango Judith A , Cranmer Lisa Marie , Huang Meei-Li , Richardson Barbra A , Boeckh Michael , John-Stewart Grace , Slyker Jennifer
Published: July 2021

Background

Cytomegalovirus (CMV) viremia is common in human immunodeficiency virus (HIV) infection and is associated with worse long-term outcomes. To date, no studies have assessed CMV viremia in children diagnosed with HIV in hospital.

Methods

We studied CMV viremia and clinical outcomes in 163 Kenyan children aged 2 months to 12 years, diagnosed with HIV in hospital. CMV DNA levels in plasma were measured using quantitative polymerase chain reaction (PCR). Regression models were used to assess associations between CMV viremia ≥1000 IU/mL and the risk of continued hospitalization or death at 15 days, duration of hospitalization, and 6-month mortality.

Results

At enrollment, 62/114 (54%) children had CMV viremia, and 20 (32%) were ≥1000 IU/mL. Eleven CMV reactivations were observed after admission. The prevalence and level of CMV viremia were highest in children <2 years and lowest in children ≥5 years old. CMV viremia ≥1000 IU/mL was independently associated with age <2 years (P = .03), higher log10 HIV RNA level (P = .01), and height-for-age z score >−2 (P = .02). Adjusting for age and log10 HIV RNA, the relative risk of death or continued hospitalization at 15 days was 1.74 (95% confidence interval [CI] = 1.04, 2.90), and the hazard ratio of 6-month mortality was 1.97 (95% CI = .57, 5.07) for children with CMV DNA ≥1000 IU/mL compared to lower-level or undetectable CMV DNA. Children with CMV DNA ≥1000 IU/mL were hospitalized a median ~5 days longer than children with lower-level or undetectable CMV DNA (P = .002).

Conclusions

In this nested observational study, CMV viremia was common in hospitalized children with HIV, and levels ≥1000 IU/mL were associated with increased risk of mortality and longer hospitalization.
Persistent Symptoms in Adult Patients 1 Year After Coronavirus Disease 2019 (COVID-19): A Prospective Cohort Study
Authors: Seeßle Jessica , Waterboer Tim , Hippchen Theresa , Simon Julia , Kirchner Marietta , Lim Adeline , Müller Barbara , Merle Uta
Published: July 2021

Background

Long COVID is defined as the persistence of symptoms beyond 3 months after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. To better understand the long-term course and etiology of symptoms we analyzed a cohort of patients with COVID-19 prospectively.

Methods

Patients were included at 5 months after acute COVID-19 in this prospective, noninterventional, follow-up study. Patients followed until 12 months after COVID-19 symptom onset (n = 96; 32.3% hospitalized, 55.2% females) were included in this analysis of symptoms, quality of life (based on an SF-12 survey), laboratory parameters including antinuclear antibodies (ANAs), and SARS-CoV-2 antibody levels.

Results

At month 12, only 22.9% of patients were completely free of symptoms and the most frequent symptoms were reduced exercise capacity (56.3%), fatigue (53.1%), dyspnea (37.5%), and problems with concentration (39.6%), finding words (32.3%), and sleeping (26.0%). Females showed significantly more neurocognitive symptoms than males. ANA titers were ≥1:160 in 43.6% of patients at 12 months post–COVID-19 symptom onset, and neurocognitive symptom frequency was significantly higher in the group with an ANA titer ≥1:160 versus <1:160. Compared with patients without symptoms, patients with ≥1 long-COVID symptom at 12 months did not differ significantly with respect to their SARS-CoV-2 antibody levels but had a significantly reduced physical and mental life quality compared with patients without symptoms.

Conclusions

Neurocognitive long-COVID symptoms can persist ≥1 year after COVID-19 symptom onset and reduce life quality significantly. Several neurocognitive symptoms were associated with ANA titer elevations. This may indicate autoimmunity as a cofactor in etiology of long COVID.
SwabExpress: An End-to-End Protocol for Extraction-Free COVID-19 Testing
Authors: Srivatsan Sanjay , Heidl Sarah , Pfau Brian , Martin Beth K , Han Peter D , Zhong Weizhi , van Raay Katrina , McDermot Evan , Opsahl Jordan , Gamboa Luis , Smith Nahum , Truong Melissa , Cho Shari , Barrow Kaitlyn A , Rich Lucille M , Stone Jeremy , Wolf Caitlin R , McCulloch Denise J , Kim Ashley E , Brandstetter Elisabeth , Sohlberg Sarah L , Ilcisin Misja , Geyer Rachel E , Chen Wei , Gehring Jase ,   Seattle Flu Study Investigators, Chu Helen Y , Boeckh Michael , Englund Janet A , Famulare Michael , Lockwood Christina M , Lutz Barry R , Nickerson Deborah A , Rieder Mark J , Starita Lea M , Thompson Matthew , Viboud Cécile , Shendure Jay , Bedford Trevor , Kosuri Sriram , Bedford Trevor , Rieder Mark J , Nickerson Deborah A , Chu Helen Y , Konnick Eric Q , Debley Jason S , Shendure Jay , Lockwood Christina M , Starita Lea M
Published: July 2021

Background

The urgent need for massively scaled clinical testing for SARS-CoV-2, along with global shortages of critical reagents and supplies, has necessitated development of streamlined laboratory testing protocols. Conventional nucleic acid testing for SARS-CoV-2 involves collection of a clinical specimen with a nasopharyngeal swab in transport medium, nucleic acid extraction, and quantitative reverse-transcription PCR (RT–qPCR). As testing has scaled across the world, the global supply chain has buckled, rendering testing reagents and materials scarce. To address shortages, we developed SwabExpress, an end-to-end protocol developed to employ mass produced anterior nares swabs and bypass the requirement for transport media and nucleic acid extraction.

Methods

We evaluated anterior nares swabs, transported dry and eluted in low-TE buffer as a direct-to-RT–qPCR alternative to extraction-dependent viral transport media. We validated our protocol of using heat treatment for viral inactivation and added a proteinase K digestion step to reduce amplification interference. We tested this protocol across archived and prospectively collected swab specimens to fine-tune test performance.

Results

After optimization, SwabExpress has a low limit of detection at 2–4 molecules/µL, 100% sensitivity, and 99.4% specificity when compared side by side with a traditional RT–qPCR protocol employing extraction. On real-world specimens, SwabExpress outperforms an automated extraction system while simultaneously reducing cost and hands-on time.

Conclusion

SwabExpress is a simplified workflow that facilitates scaled testing for COVID-19 without sacrificing test performance. It may serve as a template for the simplification of PCR-based clinical laboratory tests, particularly in times of critical shortages during pandemics.
Urinary Metabolite Biomarkers for the Detection of Synthetic Cannabinoid ADB-BUTINACA Abuse
Authors: Sia Chi Hon , Wang Ziteng , Goh Evelyn Mei Ling , Tan Yen Li , Fong Ching Yee , Moy Hooi Yan , Chan Eric Chun Yong
Published: July 2021

Background

(S)-N-(1-amino-3,3-dimethyl-1-oxobutan-2-yl)-1-butyl-1H-indazole-3carboxamide (ADB-BUTINACA) is an emerging synthetic cannabinoid that was first identified in Europe in 2019 and entered Singapore's drug scene in January 2020. Due to the unavailable toxicological and metabolic data, there is a need to establish urinary metabolite biomarkers for detection of ADB-BUTINACA consumption and elucidate its biotransformation pathways for rationalizing its toxicological implications.

Methods

We characterized the metabolites of ADB-BUTINACA in human liver microsomes using liquid chromatography Orbitrap mass spectrometry analysis. Enzyme-specific inhibitors and recombinant enzymes were adopted for the reaction phenotyping of ADB-BUTINACA. We further used recombinant enzymes to generate a pool of key metabolites in situ and determined their metabolic stability. By coupling in vitro metabolism and authentic urine analyses, a panel of urinary metabolite biomarkers of ADB-BUTINACA was curated.

Results

Fifteen metabolites of ADB-BUTINACA were identified with key biotransformations being hydroxylation, N-debutylation, dihydrodiol formation, and oxidative deamination. Reaction phenotyping established that ADB-BUTINACA was rapidly eliminated via CYP2C19-, CYP3A4-, and CYP3A5-mediated metabolism. Three major monohydroxylated metabolites (M6, M12, and M14) were generated in situ, which demonstrated greater metabolic stability compared to ADB-BUTINACA. Coupling metabolite profiling with urinary analysis, we identified four urinary biomarker metabolites of ADB-BUTINACA: 3 hydroxylated metabolites (M6, M11, and M14) and 1 oxidative deaminated metabolite (M15).

Conclusions

Our data support a panel of four urinary metabolite biomarkers for diagnosing the consumption of ADB-BUTINACA.
Parallel Divide-and-Conquer Algorithms for Bubble Sort, Selection Sort and Insertion Sort
Authors: Ganapathi Pramod , Chowdhury Rezaul
Published: July 2021

We present efficient parallel recursive divide-and-conquer algorithms for bubble sort, selection sort, and insertion sort. Our algorithms have excellent data locality and are highly parallel. The computational complexity of our insertion sort is ${{\mathcal{O}}}\left ({n^{\log _2 3}}\right )$ in contrast to ${{\mathcal{O}}}\left ({n^2}\right )$ of standard insertion sort.
Dichloroacetate inhibits the degeneration of decellularized cardiovascular implants
Authors: Chekhoeva Agunda , Nakanishi Sentaro , Sugimura Yukiharu , Toshmatova Mahfuza , Assmann Anna Kathrin , Lichtenberg Artur , Akhyari Payam , Assmann Alexander
Published: July 2021

OBJECTIVES

Intima hyperplasia is a major issue of biological cardiovascular grafts resulting in progressive in vivo degeneration that particularly decreases the durability of coronary and peripheral vascular bypasses. Previously, dichloroacetate (DCA) has been reported to prevent the formation of hyperplastic intima in injured arteries. In this study, the effect of DCA on the neointima formation and degeneration of decellularized small-caliber implants was investigated in a rat model.

METHODS

Donor rat aortic grafts (n = 22) were decellularized by a detergent-based technique, surface-coated with fibronectin (50 µl ml−1, 24 h incubation) and implanted via anastomoses to the infrarenal aorta of the recipients. Rats in the DCA group (n = 12) received DCA via drinking water during the whole follow-up period (0.75 g l−1), while rats without DCA treatment served as controls (n = 10). At 2 (n = 6 + 5) and 8 (n = 6 + 5) weeks, the grafts were explanted and examined by histology and immunofluorescence.

RESULTS

Systemic DCA treatment inhibited neointima hyperplasia, resulting in a significantly reduced intima-to-media ratio (median 0.78 [interquartile range, 0.51–1.27] vs 1.49 [0.67–2.39] without DCA, P < 0.001). At 8 weeks, neointima calcification, as assessed by an established von Kossa staining-based score, was significantly decreased in the DCA group (0 [0–0.25] vs 0.63 [0.06–1.44] without DCA, P < 0.001). At 8 weeks, explanted grafts in both groups were luminally completely covered by an endothelial cell layer. In both groups, inflammatory cell markers (CD3, CD68) proved negative.

CONCLUSIONS

Systemic DCA treatment reduces adverse neointima hyperplasia in decellularized small-caliber arterial grafts, while allowing for rapid re-endothelialization. Furthermore, DCA inhibits calcification of the implants.
The forgotten role of food cultures
Authors: Bourdichon François , Arias Emmanuelle , Babuchowski Andrzej , Bückle Anne , Bello Fabio Dal , Dubois Aurélie , Fontana Alessandra , Fritz Duresa , Kemperman Rober , Laulund Svend , McAuliffe Olivia , Miks Marta Hanna , Papademas Photis , Patrone Vania , Sharma Dinesh K , Sliwinski Edward , Stanton Catherine , Von Ah Ueli , Yao Su , Morelli Lorenzo
Published: July 2021

Fermentation is one of if not the oldest food processing technique, yet it is still an emerging field when it comes to its numerous mechanisms of action and potential applications. The effect of microbial activity on the taste, bioavailability and preservation of the nutrients and the different food matrices has been deciphered by the insights of molecular microbiology. Among those roles of fermentation in the food chain, biopreservation remains the one most debated. Presumably because it has been underestimated for quite a while, and only considered – based on a food safety and technological approach – from the toxicological and chemical perspective. Biopreservation is not considered as a traditional use, where it has been by design – but forgotten – as the initial goal of fermentation. The ‘modern’ use of biopreservation is also slightly different from the traditional use, due mainly to changes in cooling of food and other ways of preservation, Extending shelf life is considered to be one of the properties of food additives, classifying – from our perspective – biopreservation wrongly and forgetting the role of fermentation and food cultures. The present review will summarize the current approaches of fermentation as a way to preserve and protect the food, considering the different way in which food cultures and this application could help tackle food waste as an additional control measure to ensure the safety of the food.
A two-stage testing strategy for detecting genes×environment interactions in association studies
Authors: Zhou Jiabin , Li Shitao , Zhou Ying , Sheng Xiaona
Published: July 2021

Identifying gene×environment (G×E) interactions, especially when rare variants are included in genome-wide association studies, is a major challenge in statistical genetics. However, the detection of G×E interactions is very important for understanding the etiology of complex diseases. Although currently some statistical methods have been developed to detect the interactions between genes and environment, the detection of the interactions for the case of rare variants is still limited. Therefore, it is particularly important to develop a new method to detect the interactions between genes and environment for rare variants. In this study, we extend an existing method of adaptive combination of P-values (ADA) and design a novel strategy (called iSADA) for testing the effects of G×E interactions for rare variants. We propose a new two-stage test to detect the interactions between genes and environment in a certain region of a chromosome or even for the whole genome. First, the score statistic is used to test the associations between trait value and the interaction terms of genes and environment and obtain the original P-values. Then, based on the idea of the ADA method, we further construct a full test statistic via the P-values of the preliminary tests in the first stage, so that we can comprehensively test the interactions between genes and environment in the considered genome region. Simulation studies are conducted to compare our proposed method with other existing methods. The results show that the iSADA has higher power than other methods in each case. A GAW17 data set is also applied to illustrate the applicability of the new method.
Horizontal transmission and recombination of Wolbachia in the butterfly tribe Aeromachini Tutt, 1906 (Lepidoptera: Hesperiidae)
Authors: Zhao Zimiao , Zhu Jianqing , Hoffmann Ary A , Cao Lijun , Shen Li , Fang Jie , Ma Shuojia , Liu Qunxiu , Yu Weidong , Tang Liying , Wang Yongqiang , Jiang Weibin
Published: July 2021

Wolbachia is arguably one of the most ubiquitous heritable symbionts among insects and understanding its transmission dynamics is crucial for understanding why it is so common. While previous research has studied the transmission pathways of Wolbachia in several insect lineages including Lepidoptera, this study takes advantage of data collected from the lepidopteran tribe Aeromachini in an effort to assess patterns of transmission. Twenty-one of the 46 species of Aeromachini species were infected with Wolbachia. Overall, 25% (31/125) of Aeromachini specimens tested were Wolbachia positive. All Wolbachia strains were species-specific except for the wJho strain which appeared to be shared by three host species with a sympatric distribution based on a cophylogenetic comparison between Wolbachia and the Aeromachini species. Two tests of phylogenetic congruence did not find any evidence for cospeciation between Wolbachia strains and their butterfly hosts. The cophylogenetic comparison, divergence time estimation, and Wolbachia recombination analysis revealed that Wolbachia acquisition in Aeromachini appears to have mainly occurred mainly through horizontal transmission rather than codivergence.
NPSV: A simulation-driven approach to genotyping structural variants in whole-genome sequencing data
Authors: Linderman Michael D , Paudyal Crystal , Shakeel Musab , Kelley William , Bashir Ali , Gelb Bruce D
Published: July 2021

Background

Structural variants (SVs) play a causal role in numerous diseases but are difficult to detect and accurately genotype (determine zygosity) in whole-genome next-generation sequencing data. SV genotypers that assume that the aligned sequencing data uniformly reflect the underlying SV or use existing SV call sets as training data can only partially account for variant and sample-specific biases.

Results

We introduce NPSV, a machine learning–based approach for genotyping previously discovered SVs that uses next-generation sequencing simulation to model the combined effects of the genomic region, sequencer, and alignment pipeline on the observed SV evidence. We evaluate NPSV alongside existing SV genotypers on multiple benchmark call sets. We show that NPSV consistently achieves or exceeds state-of-the-art genotyping accuracy across SV call sets, samples, and variant types. NPSV can specifically identify putative de novo SVs in a trio context and is robust to offset SV breakpoints.

Conclusions

Growing SV databases and the increasing availability of SV calls from long-read sequencing make stand-alone genotyping of previously identified SVs an increasingly important component of genome analyses. By treating potential biases as a “black box” that can be simulated, NPSV provides a framework for accurately genotyping a broad range of SVs in both targeted and genome-scale applications.
How can constitutional review experiments fail? Lessons from the 1925 Chilean Constitution
Authors: Verdugo Sergio
Published: July 2021

Constitutional designers establishing a new judicial review mechanism can fail to make that mechanism a relevant instrument for checking the power of incumbent legislators or presidents. Judges may refuse to exercise their newly established powers, politicians may refuse to obey their rulings, or the judiciary may be packed, among other possible reasons. The causes can be attributed to the existence of a dominant party system, the lack of political competition, problems of institutional design, or judicial culture. This article contributes to the understanding of this problem by exploring the failed constitutional mechanism that Chilean constitutional designers established in 1925. The 1925 Chilean Constitution established the power of judicial review of legislation for the first time in Chile’s history, but the Supreme Court generally avoided to be involved in political battles. Chile had a competitive political system with frequent and regular rotation in power. The literature claims that, under these conditions, we should expect judges to be more independent and empowered, but this is not what happened in the Chilean case. Scholars studying this period of Chilean constitutional history generally associate the passivity of the Supreme Court with a legalistic culture promoting an apolitical and formalistic judicial behavior. This article claims that the narrative of judicial apoliticism served to justify, and perhaps to persuade, the Supreme Court’s choice not to intervene in politics, but more attention needs to be given to the institutional weaknesses of the judiciary of that time and to the possible strategic judicial choice.
The Varieties of Coups D’état: Introducing the Colpus Dataset
Authors: Chin John J , Carter David B , Wright Joseph G
Published: July 2021

Interest in authoritarian politics and democratic breakdown has fueled a revival in scholarship on coups d'état. However, this research is held back by the fact that no global coup dataset captures theoretically salient information on the identity of coup-makers, their goals, and the relationship between the coup leaders and the ruling regime. We introduce the Colpus dataset, new global data on coup types and characteristics in the post–World War II era. These data introduce a typology of coups, measurement strategy, and coding procedures to differentiate between whether coups seek to preserve existing ruling coalitions (leader reshuffling coups) or significantly alter ruling coalitions (regime change coups). We show trends in coup types across time and space. Finally, we demonstrate that poverty—an established determinant of coups—only predicts regime change coups. Colpus data will be of use to scholars of political instability and conflict, regime change, leadership accountability, the political economy of democracy and dictatorship, and related topics.
Estimated pill intake with on-demand PrEP with oral TDF/FTC using TFV-DP concentration in dried blood spots in the ANRS IPERGAY trial
Authors: Goldwirt Lauriane , Bauer Rebecca , Liegeon Geoffroy , Charreau Isabelle , Delaugerre Constance , Cotte Laurent , Pialou Gilles , Cua Eric , Laghzal Aïcha , Buschman Lane , Anderson Peter L , Mourah Samia , Meyer Laurence , Molina Jean-Michel ,   the ANRS IPERGAY Study Group
Published: July 2021

Background

Tenofovir diphosphate (TFV-DP) concentration in dried blood spots (DBSs) is a reliable pharmacokinetics biomarker of adherence to tenofovir disoproxil fumarate (TDF). We aimed to use DBSs to estimate pill intake among participants using on-demand pre-exposure prophylaxis (PrEP) and to identify predictive factors associated with higher TFV-DP concentrations.

Methods

DBSs were collected at the last study visit of the open-label phase of the ANRS IPERGAY study, assessing on-demand oral TDF/emtricitabine for PrEP among MSM and transgender female participants. We quantified TFV-DP in DBSs centrally. We assessed correlation between pill count and TFV-DP concentration by Spearman correlation and explored associations between participant demographics, sexual behaviour and PrEP use during sexual intercourse (SI) with TFV-DP concentrations by univariate and multivariate logistic regression models.

Results

The median age of the 245 participants included in this study was 40 years, with a median body weight of 73 kg. Median (IQR) TFV-DP concentration reached 517 (128–868) fmol/punch, corresponding to an estimated intake of 8–12 tablets per month (2–3 doses per week). Only 39% of participants had a TFV-DP concentration above 700 fmol/punch. TFV-DP concentrations were moderately correlated with pill count (r: 0.59; P < 0.001). In multivariate analysis, only systematic use of PrEP during SI and more frequent episodes of SI in the past 4 weeks were significantly associated with higher TFV-DP levels [OR (95% CI): 11.30 (3.62–35.33) and 1.46 (1.19–1.79), respectively; P < 0.001].

Conclusions

Among participants using on-demand PrEP, estimated pill intake reached 8–12 tablets per month and was correlated with frequency and systematic use of PrEP for SI.
Effect of feed restriction on dairy cow milk production: a review
Authors: Leduc Antoine , Souchet Sylvain , Gelé Marine , Le Provost Fabienne , Boutinaud Marion
Published: July 2021

In the dairy cow, negative energy balance affects milk yield and composition as well as animal health. Studying the effects of negative energy balance on dairy cow milk production is thus essential. Feed restriction (FR) experiments attempting to reproduce negative energy balance by reducing the quantity or quality of the diet were conducted in order to better describe the animal physiology changes. The study of FR is also of interest since with climate change issues, cows may be increasingly faced with periods of drought leading to a shortage of forages. The aim of this article is to review the effects of FR during lactation in dairy cows to obtain a better understanding of metabolism changes and how it affects mammary gland activity and milk production and composition. A total of 41 papers studying FR in lactating cows were used to investigate physiological changes induced by these protocols. FR protocols affect the entire animal metabolism as indicated by changes in blood metabolites such as a decrease in glucose concentration and an increase in non-esterified fatty acid or β-hydroxybutyrate concentrations; hormonal regulations such as a decrease in insulin and insulin-like growth factor I or an increase in growth hormone concentrations. These variations indicated a mobilization of body reserve in most studies. FR also affects mammary gland activity through changes in gene expression and could affect mammary cell turnover through cell apoptosis, cell proliferation, and exfoliation of mammary epithelial cells into milk. Because of modifications of the mammary gland and general metabolism, FR decreases milk production and can affect milk composition with decreased lactose and protein concentrations and increased fat concentration. These effects, however, can vary widely depending on the type of restriction, its duration and intensity, or the stage of lactation in which it takes place. Finally, to avoid yield loss and metabolic disorders, it is important to identify reliable biomarkers to monitor energy balance.
Evaluation of sow thermal preference across three stages of reproduction
Authors: Robbins Lindsey A , Green-Miller Angela R , Lay Donald C , Schinckel Allan P , Johnson Jay S , Gaskill Brianna N
Published: July 2021

The metabolic heat production of modern pigs has increased by an average of 16%, compared with sows of 30 years ago. Therefore, it is likely that temperature recommendations require updating to meet the needs of modern pigs. The objective of this study was to evaluate whether different reproductive stages of sows altered thermal preference and if current recommendations required updating. Twenty multiparous sows (3.4 ± 1.2 parity) in different reproductive stages (nonpregnant: n = 7; mid-gestation: 58.5 ± 5.68 d, n = 6; and late-gestation: 104.7 ± 2.8 d, n = 7) were tested. Thermal preference was individually tested, and sows could freely choose a temperature, using a thermal gradient between 10.4 and 30.5 °C. Sows were given 24 h to acclimate to the thermal apparatus. Before testing began, sows were given daily feed allotment and returned to the apparatus. Video from the 24-h test period was used to record sow behavior (time spent inactive), posture (upright and sternal and lateral lying), and location using instantaneous scan samples every 15 min. Data were analyzed using PROC MIXED procedure in SAS 9.4. A cubic regression model was used to calculate the sow’s most preferred temperature based on the location, or temperature, in which they spent the most time. The preference range was calculated using peak temperature preference ±SE for each sow. The reproductive stage altered where sows spent their time within the thermal gradient (P < 0.01). Late-gestation sows preferred cooler temperatures (14.0 °C) than mid-gestation (14.8 °C; P < 0.01) and nonpregnant sows (14.8 °C; P < 0.01). In summary, sow thermal preferences were within the lower half of the current recommended range (10 to 25 °C). This indicates that temperatures at the higher end of the recommended range could be uncomfortable to sows and that the thermal comfort zone of sows may be narrower than recommendations indicate.
Testicular Changes of Honey Bee Drones,Apis mellifera(Hymenoptera: Apidae), During Sexual Maturation
Authors: Klein Colby D , Kozii Ivanna V , Wood Sarah C , Koziy Roman V , Zabrodski Michael W , Dvylyuk Ihor , de Mattos Igor Medici , Moshynskyy Igor , Honaramooz Ali , Simko Elemir
Published: July 2021

The normal developmental anatomy and histology of the reproductive tract of the honey bee drone, Apis mellifera (Linnaeus, 1758), has been well documented. The post-emergence maturation changes of the accessory glands are likewise well understood, but the normal histological changes of the testicle undergoing physiologic atrophy are not well characterized. To address this knowledge gap, herein we describe the anatomy and sequential histological stages of normal testicular atrophy of drones sampled daily from emergence to sexual maturity in the spring (June) and early summer (July). Testicular histological changes during maturation are characterized by the following stages: I) conclusion of spermiogenesis; II) evacuation of spermatodesms from tubular lumens; III) progressive follicular cell atrophy, and IV) complete atrophy and collapse of testicular parenchyma. Tubular changes occur in a basilar to apical direction where segments closer to the vas deferens are histologically more mature than corresponding apical segments. In addition, the rate of testicular maturation was found to change with seasonal progression. This description of physiologic testicular atrophy should be useful for future studies investigating potential pathological effects of stressors on drone testes during sexual maturation.
Spray and Pour-On Acaricides Killed Tennessee (United States) Field-Collected Haemaphysalis longicornis Nymphs (Acari: Ixodidae) in Laboratory Bioassays
Authors: Butler R A , Chandler J G , Vail K M , Holderman C J , Trout Fryxell R T
Published: July 2021

Haemaphysalis longicornis Neumann (Asian longhorned tick) is an exotic and invasive tick species presenting a health and economic threat to the United States (U.S.) cattle industry due to its ability to transmit pathogens and infest hosts in large numbers. The objective of this study was to evaluate available products at causing H. longicornis mortality in a laboratory bioassay. The efficacy of products was evaluated at label rates using H. longicornis nymphs collected from a cattle farm in eastern Tennessee in two different bioassays (spray or dip) against untreated controls. After exposure, ticks were transferred to clean petri dishes and checked for mortality at 0, 1, 2, 3, 4, 21, 24, and 48 h post exposure. No mortality occurred in the untreated controls, whereas all treated ticks were dead within 24 h of exposure (P < 0.0001). These findings support the hypothesis that currently available spray and pour-on products are effective at causing H. longicornis mortality. We conclude that these acaricides can be used as a component to prevent H. longicornis dispersal and for control in the U.S.
Negotiating Refugee Deservingness at Europe’s Southern Frontier: Masculinity and Race in Sicilian Asylum Centres
Authors: Palillo Marco
Published: July 2021

By drawing on qualitative interviews with Sub-Saharan African international protection holders/seekers, conducted in Sicily in 2016/2017, the article investigates how hierarchies of deservingness reactivated by the refugee ‘crisis’ discourse are reproduced in small communities where all-male refugee centres are located and what the effects of these are on day-to-day interactions between refugees and Sicilians in the locale. In particular, I argue that gender hierarchies reproduced by the refugee regime intersect with racist and patriarchal structures outside the centre, resulting in the placement of refugee men at the bottom of any hierarchy of deservingness in the locale. This frames specific ways in which all-male refugee centres are interpreted as enclaves of Othered masculinities due to their assumed bogusness. Thus, participants’ masculinity emerges as a key site for locally negotiating boundaries of inclusion/exclusion between black and white communities—both inside and outside the centre.
Management and positive outcome of skull-base ossifying fibroma: a case report
Authors: El Akroud Sofia , Dokponou Yao Christian Hugues , El Mostarchid Mamoune , Chahdi Hafsa , El Asri Abad Cherif , Gazzaz Miloudi
Published: July 2021

The ossifying fibroma is a benign fibro-osseous tumor rarely affecting the skull base. The incidence of ossifying fibroma itself is uncommon. It is considered to be an aggressive and fast-growing bone lesion. Early detection and complete surgical removal are essential to deal with its aggressive nature and recurrence. We report a case of a 20-year-old man admitted for the management of ossifying fibroma of the orbital roof extending inside the orbit mimicking meningioma and revealed by a progressive proptosis and headache. The patient underwent surgery for the subtotal removal of the tumor with its frontal infiltration with a good outcome. All meningiomas like tumors are not meningiomas and other tumors such as ossifying fibroma might be mistaken for meningioma and even get confirmation from the pathological anatomy study. Need be for these tumors to be looked at more closely for better therapeutic decision-making.
Cybersecurity risks and recommendations for international travellers
Authors: Parsons Fintan J , Pantridge Michael J , Flaherty Gerard T
Published: July 2021

The unfamiliarity of their surroundings and dependence on internet connections make international travellers particularly vulnerable to cybercrime. Travel medicine clinicians should be aware of the cybersecurity threats facing travellers and be able to recognize particularly vulnerable travellers. This article outlines these threats and provides advice to mitigate them.
Clinical Characteristics and Resistance Patterns of Pseudomonas aeruginosa Isolated From Combat Casualties
Authors: Ford Mary B , Mende Katrin , Kaiser Susan J , Beckius Miriam L , Lu Dan , Stam Jason , Li Ping , Stewart Laveta , Tribble David R , Blyth Dana M
Published: July 2021

Introduction

Multidrug-resistant (MDR) Gram-negative infections complicate care of combat casualties. We describe the clinical characteristics, resistance patterns, and outcomes of Pseudomonas aeruginosa infections in combat casualties.

Methods

Combat casualties included in the Trauma Infectious Disease Outcomes Study with infections with and without P. aeruginosa isolation during initial hospitalization were compared. Pseudomonas aeruginosa from initial wound, blood, and serial isolates (≥7 days from previous isolate) collected from June 2009 through February 2014 was subjected to antimicrobial susceptibility testing, pulsed-field gel electrophoresis, and whole genome sequencing for assessing clonality. Multidrug resistance was determined using the CDC National Healthcare Safety Network definition.

Results

Of 829 combat casualties with infections diagnosed during initial hospitalization, 143 (17%) had P. aeruginosa isolated. Those with P. aeruginosa were more severely injured (median Injury Severity Score 33 [interquartile range (IQR) 27-45] vs 30 [IQR 18.5-42]; P < .001), had longer hospitalizations (median 58.5 [IQR 43-95] vs 38 [IQR 26-56] days; P < .001), and higher mortality (6.9% vs 1.5%; P < .001) than those with other organisms. Thirty-nine patients had serial P. aeruginosa isolation (median 2 subsequent isolates; IQR: 1-5), with decreasing antimicrobial susceptibility. Ten percent of P. aeruginosa isolates were MDR, associated with prior exposure to antipseudomonal antibiotics (P = .002), with amikacin and colistin remaining the most effective antimicrobials. Novel antimicrobials targeting MDR Gram-negative organisms were also examined, and 100% of the MDR P. aeruginosa isolates were resistant to imipenem/relabactam, while ceftazidime/avibactam and ceftolozane/tazobactam were active against 35% and 56% of the isolates, respectively. We identified two previously unrecognized P. aeruginosa outbreaks involving 13 patients.

Conclusions

Pseudomonas aeruginosa continues to be a major cause of morbidity, affecting severely injured combat casualties, with emergent antimicrobial resistance upon serial isolation. Among MDR P. aeruginosa, active antimicrobials remain the oldest and most toxic. Despite ongoing efforts, outbreaks are still noted, reinforcing the crucial role of antimicrobial stewardship and infection control.
A model for the formation of stellar associations and clusters from giant molecular clouds
Authors: Grudić Michael Y , Diederik Kruijssen J M , Faucher-Giguère Claude-André , Hopkins Philip F , Ma Xiangcheng , Quataert Eliot , Boylan-Kolchin Michael
Published: July 2021

We present a large suite of magnetohydrodynamic simulations of turbulent, star-forming giant molecular clouds (GMCs) with stellar feedback, extending previous work by simulating 10 different random realizations for each point in the parameter space of cloud mass and size. It is found that once the clouds disperse due to stellar feedback, both self-gravitating star clusters and unbound stars generally remain, which arise from the same underlying continuum of substructured stellar density, i.e. the hierarchical cluster formation scenario. The fraction of stars that are born within gravitationally bound star clusters is related to the overall cloud star formation efficiency set by stellar feedback, but has significant scatter due to stochastic variations in the small-scale details of the star-forming gas flow. We use our numerical results to calibrate a model for mapping the bulk properties (mass, size, and metallicity) of self-gravitating GMCs on to the star cluster populations they form, expressed statistically in terms of cloud-level distributions. Synthesizing cluster catalogues from an observed GMC catalogue in M83, we find that this model predicts initial star cluster masses and sizes that are in good agreement with observations, using only standard IMF and stellar evolution models as inputs for feedback. Within our model, the ratio of the strength of gravity to stellar feedback is the key parameter setting the masses of star clusters, and of the various feedback channels direct stellar radiation (photon momentum and photoionization) is the most important on GMC scales.
Clusters’ far-reaching influence on narrow-angle tail radio galaxies
Authors: de Vos K , Hatch N A , Merrifield M R , Mingo B
Published: July 2021

In order to study the ram-pressure interaction between radio galaxies and the intracluster medium, we analyse a sample of 208 highly bent narrow-angle tail radio sources (NATs) in clusters, detected by the Low-Frequency Array Two-metre Sky Survey. For NATs within $7R_{500}$ of the cluster centre, we find that their tails are distributed anisotropically with a strong tendency to be bent radially away from the cluster, which suggests that they are predominantly on radially inbound orbits. Within $0.5R_{500}$, we also observe an excess of NATs with their jets bent towards the cluster core, indicating that these outbound sources fade away soon after passing pericentre. For the subset of NATs with spectroscopic redshifts, we find the radial bias in the jet angles exists even out to $10R_{500}$, far beyond the virial radius. The presence of NATs at such large radii implies that significant deceleration of the accompanying inflowing intergalactic medium must be occurring there to create the ram pressure that bends the jets, and potentially even triggers the radio source.
Recognition of single-stranded nucleic acids by small-molecule splicing modulators
Authors: Tang Zhichao , Akhter Sana , Ramprasad Ankita , Wang Xiao , Reibarkh Mikhail , Wang Jinan , Aryal Sadikshya , Thota Srinivas S , Zhao Junxing , Douglas Justin T , Gao Philip , Holmstrom Erik D , Miao Yinglong , Wang Jingxin
Published: July 2021

Risdiplam is the first approved small-molecule splicing modulator for the treatment of spinal muscular atrophy (SMA). Previous studies demonstrated that risdiplam analogues have two separate binding sites in exon 7 of the SMN2 pre-mRNA: (i) the 5′-splice site and (ii) an upstream purine (GA)-rich binding site. Importantly, the sequence of this GA-rich binding site significantly enhanced the potency of risdiplam analogues. In this report, we unambiguously determined that a known risdiplam analogue, SMN-C2, binds to single-stranded GA-rich RNA in a sequence-specific manner. The minimum required binding sequence for SMN-C2 was identified as GAAGGAAGG. We performed all-atom simulations using a robust Gaussian accelerated molecular dynamics (GaMD) method, which captured spontaneous binding of a risdiplam analogue to the target nucleic acids. We uncovered, for the first time, a ligand-binding pocket formed by two sequential GAAG loop-like structures. The simulation findings were highly consistent with experimental data obtained from saturation transfer difference (STD) NMR and structure-affinity-relationship studies of the risdiplam analogues. Together, these studies illuminate us to understand the molecular basis of single-stranded purine-rich RNA recognition by small-molecule splicing modulators with an unprecedented binding mode.
Outcome of Debridement, Antibiotics, and Implant Retention for Staphylococcal Hip and Knee Prosthetic Joint Infections, Focused on Rifampicin Use: A Systematic Review and Meta-Analysis
Authors: Scheper H , Gerritsen L M , Pijls B G , Van Asten S A , Visser L G , De Boer M G J
Published: July 2021

The treatment of staphylococcal prosthetic joint infection (PJI) with debridement, antibiotics, and retention of the implant (DAIR) often results in failure. An important evidence gap concerns the treatment with rifampicin for PJI. A systematic review and meta-analysis were conducted to assess the outcome of staphylococcal hip and/or knee PJI after DAIR, focused on the role of rifampicin. Studies published until September 2, 2020 were included. Success rates were stratified for type of joint and type of micro-organism. Sixty-four studies were included. The pooled risk ratio for rifampicin effectiveness was 1.10 (95% confidence interval, 1.00–1.22). The pooled success rate was 69% for Staphylococcus aureus hip PJI, 54% for S aureus knee PJI, 83% for coagulase-negative staphylococci (CNS) hip PJI, and 73% for CNS knee PJI. Success rates for MRSA PJI (58%) were similar to MSSA PJI (60%). The meta-analysis indicates that rifampicin may only prevent a small fraction of all treatment failures.
Time to Save Time: Beneficial Effects of Blood Flow Restriction Training and the Need to Quantify the Time Potentially Saved by Its Application During Musculoskeletal Rehabilitation
Authors: Bielitzki Robert , Behrendt Tom , Behrens Martin , Schega Lutz
Published: July 2021

The main goal of musculoskeletal rehabilitation is to achieve the pre-injury and/or pre-surgery physical function level with a low risk of re-injury. Blood flow restriction (BFR) training is a promising alternative to conventional therapy approaches during musculoskeletal rehabilitation because various studies support its beneficial effects on muscle mass, strength, aerobic capacity, and pain perception. In this perspective article, we used an evidence-based progressive model of a rehabilitative program that integrated BFR in 4 rehabilitation phases: (1) passive BFR, (2) BFR combined with aerobic training, (3) BFR combined with low-load resistance training, and (4) BFR combined with low-load resistance training and traditional high-load resistance training. Considering the current research, we propose that a BFR-assisted rehabilitation has the potential to shorten the time course of therapy to reach the stage where the patient is able to tolerate resistance training with high loads. The information and arguments presented are intended to stimulate future research, which compares the time to achieve rehabilitative milestones and their physiological bases in each stage of the musculoskeletal rehabilitation process. This requires the quantification of BFR training-induced adaptations (eg, muscle mass, strength, capillary-to-muscle-area ratio, hypoalgesia, molecular changes) and the associated changes in performance with a high measurement frequency (≤1 week) to test our hypothesis. This information will help to quantify the time saved by BFR-assisted musculoskeletal rehabilitation. This is of particular importance for patients, because the potentially accelerated recovery of physical functioning would allow them to return to their work and/or social life earlier. Furthermore, other stakeholders in the health care system (eg, physicians, nurses, physical therapists, insurance companies) might benefit from that with regard to work and financial burden.
Associations between everyday discrimination and sleep quality and duration among African-Americans over time in the Jackson Heart Study
Authors: Johnson Dayna A , Lewis Tené T , Guo Na , Jackson Chandra L , Sims Mario , Wilson James G , Diez Roux Ana V , Williams David R , Redline Susan
Published: July 2021

Study Objectives

African-Americans have a high burden of poor sleep, yet, psychosocial determinants (e.g. discrimination) are understudied. We investigated longitudinal associations between everyday discrimination and sleep quality and duration among African-Americans (N = 3404) in the Jackson Heart Study.

Methods

At Exam 1 (2000–2004) and Exam 3 (2008–2013), participants completed the Everyday Discrimination Scale, rated their sleep quality (1 = poor to 5 = excellent), and self-reported hours of sleep. A subset of participants (N = 762) underwent 7-day actigraphy to objectively measure sleep duration and sleep quality (Sleep Exam 2012–2016). Changes in discrimination were defined as low stable (reference), increasing, decreasing, and high stable. Within-person changes in sleep from Exam 1 to Exam 3 were regressed on change in discrimination from Exam 1 to Exam 3 while adjusting for age, sex, education, income, employment, physical activity, smoking, body mass index, social support, and stress.

Results

At Exam 1, the mean age was 54.1 (12.0) years; 64% were female, mean sleep quality was 3.0 (1.1) and 54% were short sleepers. The distribution of the discrimination change trajectories were 54.1% low stable, 13.5% increasing, 14.6% decreasing, and 17.7% were high stable. Participants who were in the increasing (vs. low stable) discrimination group had greater decrease in sleep quality. There was no association between change in discrimination and change in sleep duration. Among Sleep Exam participants, higher discrimination was cross-sectionally associated with shorter self-reported sleep duration, independent of stress.

Conclusion

Discrimination is a unique stressor for African-Americans; thus, future research should identify interventions to reduce the burden of discrimination on sleep quality.
An on-line capacitor state identification method based on improved RLS
Authors: Cheng Shu , Liu Chang , Xue Shengxian , Wang Maoyu , Wu Xun , Luo Yu
Published: July 2021

As an essential part of DC-Link in the power converter, capacitor plays a crucial role in absorbing ripple current and suppressing ripple voltage. The health and residual service life of the DC-Link capacitor is one of the decisive factors for the safety, stability, and efficiency of the system in which it is located. Aiming at the shortcomings of existing methods, such as low dynamic sensitivity of data update and fluctuation of identification results, a capacitor state identification method based on improved RLS is proposed in this paper. The proposed method is optimized by introducing the forgetting factor algorithm and root means square algorithm to modify the iterative formula and final identification results. Compared with existing methods, this method can identify the capacitor's current state in real time and accurately. Finally, we successfully verified the accuracy, robustness, and adaptability of the proposed method by a series of experimental tests on a dSPACE platform.