bluuebunny/crossref_metadata_2025 huggingface dataset:
Deterring Deepfake Attacks with an Electrical Network Frequency Fingerprints Approach
Authors: Nagothu Deeraj , Xu Ronghua , Chen Yu , Blasch Erik , Aved Alexander
Published: April 2022

With the fast development of Fifth-/Sixth-Generation (5G/6G) communications and the Internet of Video Things (IoVT), a broad range of mega-scale data applications emerge (e.g., all-weather all-time video). These network-based applications highly depend on reliable, secure, and real-time audio and/or video streams (AVSs), which consequently become a target for attackers. While modern Artificial Intelligence (AI) technology is integrated with many multimedia applications to help enhance its applications, the development of General Adversarial Networks (GANs) also leads to deepfake attacks that enable manipulation of audio or video streams to mimic any targeted person. Deepfake attacks are highly disturbing and can mislead the public, raising further challenges in policy, technology, social, and legal aspects. Instead of engaging in an endless AI arms race “fighting fire with fire”, where new Deep Learning (DL) algorithms keep making fake AVS more realistic, this paper proposes a novel approach that tackles the challenging problem of detecting deepfaked AVS data leveraging Electrical Network Frequency (ENF) signals embedded in the AVS data as a fingerprint. Under low Signal-to-Noise Ratio (SNR) conditions, Short-Time Fourier Transform (STFT) and Multiple Signal Classification (MUSIC) spectrum estimation techniques are investigated to detect the Instantaneous Frequency (IF) of interest. For reliable authentication, we enhanced the ENF signal embedded through an artificial power source in a noisy environment using the spectral combination technique and a Robust Filtering Algorithm (RFA). The proposed signal estimation workflow was deployed on a continuous audio/video input for resilience against frame manipulation attacks. A Singular Spectrum Analysis (SSA) approach was selected to minimize the false positive rate of signal correlations. Extensive experimental analysis for a reliable ENF edge-based estimation in deepfaked multimedia recordings is provided to facilitate the need for distinguishing artificially altered media content.
Study on Drying Control Strategy of White Radish Slice Based on Monitoring Medium Relative Humidity
Authors: Jiang Dalong , Li Congcong , Zheng Zhian
Published: April 2022

Enhancing the drying rate and dried products quality, as well as energy efficiency, is very tempting for the drying industry. Recently, a lot of investigations have illustrated that the drying temperature, air velocity, and sample thickness have significant influences on the drying process. However, few investigations took into account the relative humidity (RH) as an important hot-air-drying parameter in the drying process. Therefore, in the current work, white radish slabs were used to explore the drying characteristics and quality under the drying condition of a constant RH, decreasing the RH step by step and decreasing RH automatically, together with a constant air velocity of 1 m·s−1 and a drying temperature of 60 °C. Compared to continuous dehumidification, the step-down RH process was conducive to the material center temperature rise in the early stage of drying. When the material central temperature was increased and then the RH was reduced, the drying rate was increased and the overall drying time was shortened. The automatic-down RH control drying process includes three dehumidification processes. The respective RH control values were 40%, 30%, and 20% and the respective durations were 180 min, 90 min, and 60 min. The comprehensive quality evaluation showed that the comprehensive score of the automatic-down RH control process at 60 °C was the highest, which was 0.85. The L* and b* values of the automatic-down RH control were 26.0 and 1.67, respectively, which were better than those of the step-down RH, constant 20% RH, and constant 40% RH. The maximum rehydration ratio was 3.96 under the automatic-down RH control condition, and the quality was good. The lowest energy consumption under the condition of the automatic-down RH control was 0.90 kW·h·kg−1. The present work contributes to a better understanding of the effect of the RH on the drying characteristics and quality of white radish slices, which is useful for enhancing the drying rate and dried products’ quality as well as energy efficiency.
The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 in Syntrichia caninervis
Authors: Zhang Yigong , Zhou Jiyang , Zhang Yi , Zhang Daoyuan
Published: April 2022

The ubiquitination pathway has been found to regulate plant responses to environmental stress. However, the role of E3 ubiquitin ligase in desiccation tolerant moss has not yet been elucidated. Previous research has shown that the abscisic acid (ABA) signaling factor ScABI3 can significantly increase desiccation tolerance and reduce ABA sensitivity in the desert moss Syntrichia caninervis. In this study, we identified a RING-type E3 ubiquitin ligase, ScPRT1, and showed that ScABI3 can directly interact with ScPRT1 in vitro and in vivo. Furthermore, we found that the high expression of ScPRT1 can interfere with the transcription of ScABI3 under ABA treatment. Therefore, we speculate that ScPRT1 may degrade ScABI3 through the ubiquitin-26S proteasome system and participate in ABA-dependent signaling in response to ABA-insensitivity or desiccation tolerance in S. caninervis. The findings from our study may enrich our knowledge of the role of E3 ubiquitin ligase in desiccation tolerance and lay a theoretical foundation for an in-depth study of the relationship between ubiquitination modification and ABA signal transduction under environmental stress.
Utilization Strategies of Two Environment Phenotypes in Genomic Prediction
Authors: Lin Qing , Teng Jinyan , Cai Xiaodian , Li Jiaqi , Zhang Zhe
Published: April 2022

Multiple environment phenotypes may be utilized to implement genomic prediction in plant breeding, while it is unclear about optimal utilization strategies according to its different availability. It is necessary to assess the utilization strategies of genomic prediction models based on different availability of multiple environment phenotypes. Here, we compared the prediction accuracy of three genomic prediction models (genomic prediction model (genomic best linear unbiased prediction (GBLUP), genomic best linear unbiased prediction (GFBLUP), and multi-trait genomic best linear unbiased prediction (mtGBLUP)) which leveraged diverse information from multiple environment phenotypes using a rice dataset containing 19 agronomic traits in two disparate seasons. We found that the prediction accuracy of genomic prediction models considering multiple environment phenotypes (GFBLUP and mtGBLUP) was better than the classical genomic prediction model (GBLUP model). The deviation of prediction accuracy of between GBLUP and mtGBLUP or GFBLUP was associated with the phenotypic correlation. In summary, the genomic prediction models considering multiple environment phenotypes (GFBLUP and mtGBLUP) demonstrated better prediction accuracy. In addition, we could utilize different genomic prediction strategies according to different availability of multiple environment phenotypes.
Epigenetic Regulation Mechanisms of the Cofilin-1 Gene in the Development and Differentiation of Bovine Primary Myoblasts
Authors: Sun Yujia , Ma Yaoyao , Zhao Tianqi , Li Mingxun , Mao Yongjiang , Yang Zhangping
Published: April 2022

As the quality of beef products has received increasing attention, it is essential to explore the underlying transcriptional and epigenetic mechanisms of meat traits. Our project uses Qinchuan cattle as the research subject. First, we examined the spatiotemporal expression pattern of the CFL1 gene in a panel of fetal bovine, calf, and adult cattle samples. Then, we performed DNA methylation experiments of CFL1 on myogenesis and muscle maturation using the BSP amplification and COBRA sequencing techniques and found that high DNA methylation levels showed low expression levels. Next, we performed an assay between bta-miR-182 and the CFL1 gene and demonstrated that miR-182 could promote bovine primary myoblast differentiation by negatively regulated the expression of CFL1. Finally, we constructed an adenovirus overexpression and interference vector and found that CFL1 could suppress the differentiation of bovine primary myoblasts. In summary, our experiment comprehensively analyzes the epigenetic regulation mechanisms of the CFL1 gene in the development and differentiation of bovine primary myoblasts. This has far-reaching significance for improving the meat production and meat quality of Qinchuan cattle. This can provide reliable data support and a theoretical research basis for the rapid and efficient breeding selection of local yellow cattle and the genetic improvement of meat quality.
Assessing the Perceptions and Impact of Critical Incident Stress Management Peer Support among Firefighters and Paramedics in Canada
Authors: Price Jill A. B. , Landry Caeleigh A. , Sych Jeff , McNeill Malcolm , Stelnicki Andrea M. , Asmundson Aleiia J. N. , Carleton R. Nicholas
Published: April 2022

Relative to the general population, public safety personnel (PSP) appear at an increased risk of developing mental health challenges as a result of repeated exposure to potentially psychologically traumatic events (PPTEs). To help mitigate the impact of PPTEs on PSP mental health, many PSP agencies have implemented diverse peer support despite limited empirical evidence. The current study was designed to expand the empirical evidence surrounding peer support by investigating one of the most widely used and structured peer support resources: Critical Incident Stress Management (CISM). Specifically, the current study with integrated firefighters and paramedics assessed (a) the prevalence of mental disorders; (b) perceptions of high fidelity CISM peer support; and (c) the comparative associations of CISM with high fidelity (n = 91) versus unknown fidelity (n = 60) versus no CISM (n = 64) and mental health. Results indicated that (a) mental disorders are prevalent among PSP irrespective of gender, age, and years of service; (b) participants perceived CISM peer support as offering beneficial and valuable tools (e.g., skills and coping strategies); and (c) high fidelity CISM environments offer some mental health benefits to individuals who screen positive for alcohol use disorder and generalized anxiety disorder. Overall, the current study offers novel information that can inform future directions for evidence-based peer support and policy decisions designed to support the mental health of PSP.
Coordinated Health in Texas Elementary Schools’ Campus Improvement Plans: Analysis of Regional Differences and Trends between 2016 and 2020
Authors: Classen Sarah , Szeszulski Jacob , Ranjit Nalini , Rivas-Ponce Genesis , Hoelscher Deanna M.
Published: April 2022

Schools signal health priorities through policies. Using a repeated cross-sectional study design, we compare the presence and strength of policies related to four topics—physical activity, nutrition, mental health, and bullying—described in elementary school Campus Improvement Plans (CIPs; also called school improvement plans) within Texas, across four Texas Public Health Regions (PHRs), and between 2016 and 2020. CIPs were collected using a multi-stage probability-based survey approach, scored using an adapted WellSAT tool, and analyzed to determine associations between PHR or year and health topic. Across 170 CIPs, bullying was the most frequently addressed topic, followed by mental health, physical activity, and nutrition. On average, schools addressed 2.7 ± 1.3 topics within their CIP; 38.2% of schools addressed all four, 26.5% addressed three, 12.4% addressed two, 15.3% addressed one, and 7.6% addressed none. CIPs in the same district had high levels of clustering (ICCs = 0.28–0.55). The mostly rural Panhandle PHR included the fewest topics in their CIPs and used the weakest policy language. Between 2016 and 2020, there was a decrease in the proportion of CIPs that addressed nutrition; the strength of language for mental health and bullying also decreased. Regional and time trends reveal opportunities for more robust school health policy interventions.
The Benefits of Physical Activity for People with Obesity, Independent of Weight Loss: A Systematic Review
Authors: Pojednic Rachele , D'Arpino Emma , Halliday Ian , Bantham Amy
Published: April 2022

Purposeful weight loss continues to be the primary focus for treating obesity. However, this strategy appears to be inadequate as obesity rates continue to rise and a myriad of benefits of physical activity that affect multiple health outcomes related to obesity and associated comorbidities are not integrated into treatment strategies. There are emerging correlational data in individuals with obesity that demonstrate physical activity can be beneficial to many critical health markers, independent of weight loss or changes in BMI. This systematic review investigates interventional studies that examine health markers, independent of weight loss, in individuals with obesity. Fourteen studies were identified that utilized a variety of physical activity interventions with primary endpoints that included cellular, metabolic, systemic and brain health outcomes. The review of the literature demonstrates that for individuals with obesity, there are both small-scale and large-scale physiologic benefits that occur with increased physical activity of various modalities. Focusing on these benefits, rather than a narrow focus of weight loss alone, may increase physical activity behavior and health for individuals with obesity.
Relationships among Sports Group Cohesion, Psychological Collectivism, Mental Toughness and Athlete Engagement in Chinese Team Sports Athletes
Authors: Gu Song , Xue Lan
Published: April 2022

Background: Cohesion is an important factor affecting sports performance. This study constructed a mediating model to explore the mechanism of cohesion toward psychological collectivism, mental toughness, and athlete engagement of Chinese team sports athletes, and to investigate the mediating effect of psychological collectivism and mental toughness on cohesion and athlete engagement. Methods: A total of 326 active Chinese athletes (54% males, 46% females) aged 14 to 26 years (M = 19.63, SD = 6.51) from eight sports were investigated by questionnaire. Results: The athlete engagement can be predicted significantly and positively by cohesion and its dimensions, and ATG-T is more important in advantage analysis. Direct and indirect paths indicate that cohesion affects athlete engagement, through the mediating effects of psychological collectivism, the mediating effects of mental toughness, the serial multiple mediating of psychological collectivism and mental toughness. The mediating effect model had a satisfactory goodness of fit and explained 50.5% of the variance in athlete engagement, and the SEM revealed the mechanism of cohesion in Chinese athlete engagement to a certain extent. Conclusion: Psychological collectivism is the embodiment of high-quality cohesion in Chinese team sports. The increase in cohesion and psychological collectivism can improve Chinese athletes’ ability to cope with stressful situations in sports, which may allow them to achieve a better performance through athlete engagement.
Childhood Maltreatment and Psychosocial Flourishing among Emerging Adults: Roles of Psychological Suzhi and Self-Esteem
Authors: Li Zewei , Pan Yangu , Liu Guangzeng , Li Bingbing , Li Xu
Published: April 2022

Studies have shown that childhood maltreatment can negatively predict psychosocial flourishing among emerging adults. However, few studies have revealed the factors that can protect the psychosocial flourishing of emerging adults who experienced maltreatment during childhood. Based on theoretical and empirical considerations, this study investigated whether and how psychological suzhi (a positive quality that can facilitate individuals’ adaptation to environment) plays a protective role in the relationship between childhood maltreatment and psychosocial flourishing among emerging adults. A total of 2863 Chinese emerging adults (Mage = 19.96 years) completed the self-report measures of the Childhood Trauma Questionnaire, Psychological Suzhi Questionnaire, Rosenberg Self-esteem Scale, and Flourishing Scale. The moderated analyses showed that childhood maltreatment had a less negative impact on psychological flourishing in high psychological suzhi emerging adults than in low psychological suzhi emerging adults. Part of the moderating effect of psychological suzhi is mediated through self-esteem. These results demonstrated that psychological suzhi plays a buffering effect between childhood maltreatment and psychosocial flourishing, and part of the effect is achieved by mediated variable self-esteem. These findings are discussed, and practical implications are presented.
Implementation of the Strategy for Breastfeeding and Complementary Feeding in the Federal District in Brazil
Authors: Moura Amanda Souza , Gubert Muriel Bauermann , Venancio Sonia Isoyama , Buccini Gabriela
Published: April 2022

Background: The Brazilian Breastfeeding and Complementary Feeding Strategy—Estratégia Amamenta e Alimenta Brasil (EAAB) aims to promote optimal breastfeeding (BF) and complementary feeding (CF) practices through the training of primary health professionals. Competition among health priorities and programs is one of the organizational contextual barriers to consolidating the implementation of the EAAB. Methods: This case study included six Primary Health Units (PHU) certified in the EAAB. Documentary analysis, interviews, and surveys were conducted, which informed a conceptual logical model. Organizational context indicators (positive and negative) were identified across the logical model based on the Matus Triangle, and they were used to analyze the degree of implementation of the EAAB in the PHUs. Results: The logic model elucidated six stages of EAAB implementation, but none on post-certification monitoring. Ten indicators positively influenced the implementation, including having legislation that prioritizes BF and CF. Seven indicators exerted negative influence, especially the lack of specific funding resources for the EAAB. Only one PHU had a consolidated degree of implementation. Conclusions: Lack of specific funding, monitoring of BF and CF practices, and compliance with certification criteria are the main challenges for the EAAB’s sustainability.
Hope and Resilience Related to Fear of COVID-19 in Young People
Authors: Javier-Aliaga David J. , Quispe Gluder , Quinteros-Zuñiga Dámaris , Adriano-Rengifo Cristian E. , White Michael
Published: April 2022

In the face of the psychological crisis of fear caused by the COVID-19 pandemic, it is relevant to know the positive impact of hope and resilience during this context. The purpose of this study was to determine the correlation between hope and resilience with fear of COVID-19 in young people. The design was non-experimental, cross-sectional, and correlational. The sample consisted of 192 young people living in Metropolitan Lima, Peru. We used the Hope-Despair Questionnaire, the Resilience Scale, and the COVID-19 Fear Questionnaire. The results show that there is a significant correlation between hope, resilience, and fear of COVID-19 in young people. On the other hand, a significant difference was found in resilience according to gender. Likewise, it was found that the variables hope and resilience explain 81% (R2 adjusted) of the fear of COVID-19 (F test = 21.53; p < 0.01). Hope and resilience are protective factors that have a positive impact when facing the fear of COVID-19. Thus, policies, programs, and public health strategies related to positive mental health should be promoted, with emphasis on hope and resilience.
Obstructive Sleep Apnoea Severity Is Negatively Associated with Depressive Symptoms: A Cross-Sectional Survey of Outpatients with Suspected Obstructive Sleep Apnoea in Japan
Authors: Ito Kazuki , Uetsu Masahiro , Ubara Ayaka , Matsuda Arichika , Sumi Yukiyoshi , Kadotani Hiroshi
Published: April 2022

Background: Multiple clinical departments are involved in the provision of obstructive sleep apnoea (OSA) therapy in Japan. Inconsistent results regarding the association between depression and OSA have been reported. Methods: This cross-sectional survey compared newly diagnosed OSA patients at two outpatient sleep apnoea units in Shiga Prefecture, Japan: one associated with the psychiatry department (n = 583), and the other with the otolaryngology department (n = 450). Results: The unit associated with the psychiatry department had more patients referred by psychiatrists than that with the otolaryngology department (11% vs. 3% p < 0.05). Sleepiness, insomnia, and depression were assessed using the Epworth Sleepiness Scale (ESS), Athens Insomnia Scale (AIS), and Patient Health Questionnaire-9 (PHQ-9), respectively. The ESS, AIS, and PHQ-9 scores were higher in the sleep unit in the psychiatry department (p < 0.001 each). Snoring and moderate to severe OSA were more prevalent in the unit attached to the otolaryngology department (p < 0.001 each). Patients with moderate to severe OSA had lower PHQ-9 scores than those with no to mild OSA (OR: 0.96, 95% CI: 0.92–1.00, p = 0.042). Conclusion: Patients with sleepiness, insomnia, and depressive symptoms were more likely to attend a sleep outpatient unit associated with a psychiatry department, whereas those with snoring and sleep apnoea attended that associated with an otolaryngology department. OSA severity was negatively associated with depressive symptoms.
Healthcare Services and Formal Caregiver’s Psychosocial Risk Factors: An Observational Study
Authors: Pereira Anabela , Brito Elisabeth , Souto Isabel , Alves Bruno
Published: April 2022

The prevention and management of chronic disease primarily requires risk reduction measures, through strategic coordination across various government areas. Recognizing that health workers and the public health workforce are integral to building strong and resilient health, the present study analyses the relation between Psychosocial Risk Factors (PRFs, to which formal caregivers are exposed in the healthcare settings), and the work system related elements of the Systems Engineering Initiative for Patient Safety (SEIPS 3.0) framework. An empirical study was carried out, involving 333 formal caregivers of healthcare services. A total of 31 PRFs were assessed (using the COPSOQ III), making it possible to find a relationship between the PRFs analyzed with three elements of the work system, namely Task (5 PRFs), Organizational factors (17 PRFs), and Individual (9 PRFs). The present work contributes not only in terms of outcomes that allow the development of mental illness prevention and mental health promotion actions for healthcare formal caregivers, but also in terms of the relevance that these factors can have on the quality of health services, as well as their users (patients), in line with SEIPS 3.0 model.
Long-Term Health Impacts of Wildfire Exposure: A Retrospective Study Exploring Hospitalization Dynamics Following the 2016 Wave of Fires in Israel
Authors: Cohen Odeya , Shapira Stav , Furman Eyal
Published: April 2022

Background: Climate-related events, including wildfires, which adversely affect human health, are gaining the growing attention of public-health officials and researchers. Israel has experienced several disastrous fires, including the wave of fires in November 2016 that led to the evacuation of 75,000 people. The fires lasted six days (22–27 November) with no loss of life or significant immediate health impacts. The objective of this study is to explore the long-term hospitalization dynamics in a population exposed to this large-scale fire, including the effects of underlying morbidity and socio-economic status (SES). Methods: This is a retrospective crossover study, conducted in 2020, analyzing the electronic medical records of residents from areas exposed to a wildfire in northern Israel. The study spans from one year before exposure to two years after it (22 November 2015–27 November 2018). The hospitalization days during the study period were analyzed using the Poisson regression model. The rate of hospitalization days along with 95% confidence intervals (CIs) were plotted. Results: The study included 106,595 participants. The median age was 37 (IQR = 17–56), with a mean socio-economic ranking of 6.47 out of 10 (SD = 2.01). Analysis revealed that people with underlying morbidity were at greater risk of experiencing long-term effects following fires, which was manifested in higher hospitalization rates that remained elevated for two years post-exposure. This was also evident among individuals of low socio-economic status without these background illnesses. Conclusions: Healthcare services should prepare for increased hospitalization rates during the two years following wildfires for populations with underlying morbidity and those of low socio-economic status. Implementing preventive-medicine approaches may increase the resiliency of communities in the face of extreme climate-related events and prevent future health burdens. Additional research should focus on the specific mechanisms underpinning the long-term effects of wildfire exposure.
Peer Support for Public Safety Personnel in Canada: Towards a Typology
Authors: Price Jill A. B. , Ogunade Adeyemi O. , Fletcher Amber J. , Ricciardelli Rosemary , Anderson Gregory S. , Cramm Heidi , Carleton R. Nicholas
Published: April 2022

Public safety personnel (PSP) are frequently exposed to potentially psychologically traumatic events (PPTEs) which can impact mental health. To help mitigate the negative effects of PPTEs, PSP commonly rely on peer support. Peer support generally refers to a wide variety of mental health resources that offer social or emotional assistance to a peer, and in some cases professional assistance. Despite the use of and demand for peer support, there is relatively little empirical evidence regarding effectiveness. The evidence gaps regarding peer support effectiveness may be due, in part, to inadequate guidelines and standards of practice that are publicly supported by a consensus among peer support providers. The current study was designed to explore the current conceptualization and implementation of peer support among Canadian PSP using a document analysis. The results indicate that peer support can be conceptualized via three models (i.e., peer-enabled, peer-led, peer-partnership) and implemented via two delivery methods (i.e., program, service). The research team proposed a novel diagram towards a typology of peer support to highlight the diversity in peer support conceptualization and implementation and provide a foundation for the development of mutually agreed-upon language and a shared framework. Overall, the current study can help inform peer support resources within and beyond PSP communities.
Analysis of Factors Influencing Polish Students’ Opinions on Euthanasia
Authors: Stokłosa Iga , Stokłosa Maciej , Więckiewicz Gniewko , Porwolik Mateusz , Bugajski Maciej , Masarczyk Wilhelm , Męcik-Kronenberg Tomasz , Piegza Magdalena , Pudlo Robert , Gorczyca Piotr
Published: April 2022

Due to the continuous development of palliative care and methods of pain relief in the last moments of patients’ lives, we are faced with the question of how long we should take measures to delay inevitable death for, without providing prolonged therapy against the patient’s will. For terminally ill and people experiencing unimaginable suffering, euthanasia is considered as one of the possible options for a dignified farewell. The aim of the study was to determine the views on euthanasia in a group of students from Polish universities. Questionnaire responses were analyzed for 9686 students (79.9% of women and 20.1% of men) aged 18–35 years. Respondents were asked to complete their own questionnaire on demographic data and attitudes toward euthanasia in the case of severe terminal illness or paralysis preventing independent living. Euthanasia was significantly more acceptable among women (85% vs. 75%, p < 0.0001; 69% vs. 62%, p < 0.0001) and non-believers [98% vs. 97% (denominations other than Christian), 84% (other forms of Christianity), 69% (Roman Catholic); p < 0.0001] in every case considered. Religious affiliation was the factor that most influenced attitudes toward euthanasia. Among the other elements influencing attitudes toward euthanasia, residence and field of study were distinguished. Considering the large sample size and specific conclusions, the article should be an important argument in the discussion on euthanasia.
Postural Balance in Relation with Vision and Physical Activity in Healthy Young Adults
Authors: Onofrei Roxana Ramona , Amaricai Elena
Published: April 2022

Postural balance is an essential part of a wide range of activities, from daily living tasks to sports. Regularly repeated physical and/or sport activities improve both the postural performance and the postural strategy. The aim of our study was to evaluate if the physical activity level is a factor that influences postural balance performance, including the impact of vision and gender, in healthy young adults. Postural balance was assessed in 78 subjects (38 males and 40 females, aged 20.64 ± 1.18 years) by using the PoData system, in open (EO) and closed (EC) eye conditions. Based on the physical activity level, subjects were classified in two groups—low physical activity level (n = 36, 46.15%) and moderate physical activity level (n = 42, 53.85%). A group significant difference was found only for the average centre of pressure (CoP) deviations on the latero-lateral axis (CoPX), with a higher lateral deviation of the CoP (toward right) in the low physical activity group (F = 4.005, p = 0.04). CoP path length, the 90% confidence ellipse and maximum CoP speed were significantly increased in EC conditions. A statistically significant interaction effect (vision × physical activity) was observed for the CoP path length (F = 7.9, p = 0.006).
How Does Public Health Investment Affect Subjective Well-Being? Empirical Evidence from China
Authors: Yang Yingzhu , Zhao Lexiang , Cui Feng
Published: April 2022

Maximizing or improving residents’ subjective well-being is one of the basic purposes of public expenditure. As an important component of public expenditure, the impact of public health investment on residents’ subjective well-being receives considerable attention. Regarding the empirical evidence, this paper measures residents’ subjective well-being from the perspectives of overall cognitive happiness, life satisfaction, positive emotions and negative emotions, on the basis of a multi-level structural model of subjective well-being. Factor analysis is used to estimate the subjective well-being of residents at the province level in China, based on the China Family Panel Studies of 2018. In addition, structural equation modeling is employed to explore the impact of public health investment and its regional disparity on the subjective well-being of residents. The empirical results show that public health investment has a significant positive effect on residents’ subjective well-being. Moreover, there is an inverted U-shaped relationship between the regional disparity of public health investment and residents’ subjective well-being. Further study illustrates that the effects of public health investment and its regional disparity on residents’ subjective well-being are heterogeneous by group. Public health investment has a greater impact on the well-being of low- and middle-income, eastern and urban residents than high-income, midwest and rural residents.
Sex-Specific Association between Environmental Tobacco Smoke Exposure and Asthma Severity among Adults with Current Asthma
Authors: Becerra Benjamin J. , Arias Devin , Becerra Monideepa B.
Published: April 2022

Background: Tobacco smoke has been associated with negative health outcomes, including those with chronic respiratory illnesses, such as asthma. This study aimed to assess the relationship between exposure to environmental tobacco smoke (ETS), as well as tobacco use (cigarette and electronic cigarettes), on asthma severity among adults with current asthma, with stratification by sex to understand potential biological sex differences. Methods: The study population consisted of Californian adults 18 years or older with self-reported physician/health care diagnosis of asthma and still having current asthma from 2020 California Health Interview Survey. All descriptive statistics and analyses were sex-stratified and survey-weighted. Crosstabulations were used to understand the association between asthma attack and ETS or firsthand smoke exposure, while binary logistic regression models were used to assess the effect of ETS exposure, current smoking status, and control variables on asthma attack in the past 12 months, with a sub-analysis among non-smoking adults with asthma. Results: Among the primary variable of interest, 35% of males and 30% of females reported ETS exposure in the past 12 months, while 13% of males and 6% of females reported being a current smoker. Past year asthma attack was reported among 43% and 55% of males and females, respectively. Among males, after adjusting for all control variables, asthma attack was significantly higher among those with ETS exposure (OR: 1.75, 95% CI: 1.01–3.02) and among current smokers (OR: 3.82, 95% CI: 1.49, 9.81). Male non-smokers with ETS exposure had a 109% higher odds of asthma attack, compared to non-exposure individuals. Conclusion: Using a population-based survey, our results highlight the ongoing burden of tobacco use and exposure particularly among males with current asthma, further corroborate the literature on the relationship between tobacco and asthma, and highlight putative sex-specific outcomes.
The Thousand Polish Genomes—A Database of Polish Variant Allele Frequencies
Authors: Kaja Elżbieta , Lejman Adrian , Sielski Dawid , Sypniewski Mateusz , Gambin Tomasz , Dawidziuk Mateusz , Suchocki Tomasz , Golik Paweł , Wojtaszewska Marzena , Mroczek Magdalena , Stępień Maria , Szyda Joanna , Lisiak-Teodorczyk Karolina , Wolbach Filip , Kołodziejska Daria , Ferdyn Katarzyna , Dąbrowski Maciej , Woźna Alicja , Żytkiewicz Marcin , Bodora-Troińska Anna , Elikowski Waldemar , Król Zbigniew J. , Zaczyński Artur , Pawlak Agnieszka , Gil Robert , Wierzba Waldemar , Dobosz Paula , Zawadzka Katarzyna , Zawadzki Paweł , Sztromwasser Paweł
Published: April 2022

Although Slavic populations account for over 4.5% of world inhabitants, no centralised, open-source reference database of genetic variation of any Slavic population exists to date. Such data are crucial for clinical genetics, biomedical research, as well as archeological and historical studies. The Polish population, which is homogenous and sedentary in its nature but influenced by many migrations of the past, is unique and could serve as a genetic reference for the Slavic nations. In this study, we analysed whole genomes of 1222 Poles to identify and genotype a wide spectrum of genomic variation, such as small and structural variants, runs of homozygosity, mitochondrial haplogroups, and de novo variants. Common variant analyses showed that the Polish cohort is highly homogenous and shares ancestry with other European populations. In rare variant analyses, we identified 32 autosomal-recessive genes with significantly different frequencies of pathogenic alleles in the Polish population as compared to the non-Finish Europeans, including C2, TGM5, NUP93, C19orf12, and PROP1. The allele frequencies for small and structural variants, calculated for 1076 unrelated individuals, are released publicly as The Thousand Polish Genomes database, and will contribute to the worldwide genomic resources available to researchers and clinicians.
Serine Hydroxymethyltransferase 1 Is Essential for Primary-Root Growth at Low-Sucrose Conditions
Authors: Yuan Yang , Xu Danyun , Xiang Denghao , Jiang Li , Hu Honghong
Published: April 2022

Plant roots are essential organs for absorbing nutrients from the soil or medium. Sucrose functions as a vital carbon source in root development, and sucrose starvation interferes with the redox state of plant cells. However, the mechanism of root growth at sucrose starvation remains unclear. Here, we report that SHMT1 (serine hydroxymethyltransferase 1) plays a crucial role in primary-root growth. SHMT1 mutation caused decreased sugar levels, excessive H2O2 accumulation, and severe root-growth arrest at sucrose-free conditions, whereas plants with SHMT1 overexpression had increased sugar and decreased H2O2 levels, and longer primary roots. Sucrose supply fully restored root growth of shm1-2, but CO2 alone could not, and SHMT1 is much more stable in roots than shoots at sucrose conditions, suggesting that SHMT1 accumulation in roots is critical for sucrose accumulation and root growth. Further ROS scavenging by GSH application or ROS synthesis inhibition by apocynin application or RBOHD mutation reduced H2O2 levels and partially restored the root-growth arrest phenotype of shm1-2 at low-sucrose conditions, suggesting that SHMT1 modulates root growth via sucrose-mediated ROS accumulation. Our findings demonstrated the role of SHMT1 in primary-root growth by regulating sucrose accumulation and ROS homeostasis in roots.
Systematic Analysis and Identification of Drought-Responsive Genes of the CAMTA Gene Family in Wheat (Triticum aestivum L.)
Authors: Wang Dezhou , Wu Xian , Gao Shiqin , Zhang Shengquan , Wang Weiwei , Fang Zhaofeng , Liu Shan , Wang Xiaoyan , Zhao Changping , Tang Yimiao
Published: April 2022

The calmodulin-binding transcription activator (CAMTA) is a Ca2+/CaM-mediated transcription factor (TF) that modulates plant stress responses and development. Although the investigations of CAMTAs in various organisms revealed a broad range of functions from sensory mechanisms to physiological activities in crops, little is known about the CAMTA family in wheat (Triticum aestivum L.). Here, we systematically analyzed phylogeny, gene expansion, conserved motifs, gene structure, cis-elements, chromosomal localization, and expression patterns of CAMTA genes in wheat. We described and confirmed, via molecular evolution and functional verification analyses, two new members of the family, TaCAMTA5-B.1 and TaCAMTA5-B.2. In addition, we determined that the expression of most TaCAMTA genes responded to several abiotic stresses (drought, salt, heat, and cold) and ABA during the seedling stage, but it was mainly induced by drought stress. Our study provides considerable information about the changes in gene expression in wheat under stress, notably that drought stress-related gene expression in TaCAMTA1b-B.1 transgenic lines was significantly upregulated under drought stress. In addition to providing a comprehensive view of CAMTA genes in wheat, our results indicate that TaCAMTA1b-B.1 has a potential role in the drought stress response induced by a water deficit at the seedling stage.
Immune Signature of COVID-19: In-Depth Reasons and Consequences of the Cytokine Storm
Authors: Niedźwiedzka-Rystwej Paulina , Majchrzak Adam , Kurkowska Sara , Małkowska Paulina , Sierawska Olga , Hrynkiewicz Rafał , Parczewski Miłosz
Published: April 2022

In the beginning of the third year of the fight against COVID-19, the virus remains at least still one step ahead in the pandemic “war”. The key reasons are evolving lineages and mutations, resulting in an increase of transmissibility and ability to evade immune system. However, from the immunologic point of view, the cytokine storm (CS) remains a poorly understood and difficult to combat culprit of the extended number of in-hospital admissions and deaths. It is not fully clear whether the cytokine release is a harmful result of suppression of the immune system or a positive reaction necessary to clear the virus. To develop methods of appropriate treatment and therefore decrease the mortality of the so-called COVID-19-CS, we need to look deeply inside its pathogenesis, which is the purpose of this review.
Density Scaling of Translational and Rotational Molecular Dynamics in a Simple Ellipsoidal Model near the Glass Transition
Authors: Liszka Karol , Grzybowski Andrzej , Koperwas Kajetan , Paluch Marian
Published: April 2022

In this paper, we show that a simple anisotropic model of supercooled liquid properly reflects some density scaling properties observed for experimental data, contrary to many previous results obtained from isotropic models. We employ a well-known Gay–Berne model earlier parametrized to achieve a supercooling and glass transition at zero pressure to find the point of glass transition and explore volumetric and dynamic properties in the supercooled liquid state at elevated pressure. We focus on dynamic scaling properties of the anisotropic model of supercooled liquid to gain a better insight into the grounds for the density scaling idea that bears hallmarks of universality, as follows from plenty of experimental data collected near the glass transition for different dynamic quantities. As a result, the most appropriate values of the scaling exponent γ are established as invariants for a given anisotropy aspect ratio to successfully scale both the translational and rotational relaxation times considered as single variable functions of densityγ/temperature. These scaling exponent values are determined based on the density scaling criterion and differ from those obtained in other ways, such as the virial–potential energy correlation and the equation of state derived from the effective short-range intermolecular potential, which is qualitatively in accordance with the results yielded from experimental data analyses. Our findings strongly suggest that there is a deep need to employ anisotropic models in the study of glass transition and supercooled liquids instead of the isotropic ones very commonly exploited in molecular dynamics simulations of supercooled liquids over the last decades.
Atomic-Resolution Structures and Mode of Action of Clinically Relevant Antimicrobial Peptides
Authors: Bhattacharjya Surajit , Mohid Sk Abdul , Bhunia Anirban
Published: April 2022

Global rise of infections and deaths caused by drug-resistant bacterial pathogens are among the unmet medical needs. In an age of drying pipeline of novel antibiotics to treat bacterial infections, antimicrobial peptides (AMPs) are proven to be valid therapeutics modalities. Direct in vivo applications of many AMPs could be challenging; however, works are demonstrating encouraging results for some of them. In this review article, we discussed 3-D structures of potent AMPs e.g., polymyxin, thanatin, MSI, protegrin, OMPTA in complex with bacterial targets and their mode of actions. Studies on human peptide LL37 and de novo-designed peptides are also discussed. We have focused on AMPs which are effective against drug-resistant Gram-negative bacteria. Since treatment options for the infections caused by super bugs of Gram-negative bacteria are now extremely limited. We also summarize some of the pertinent challenges in the field of clinical trials of AMPs.
Crosstalk between β-Catenin and CCL2 Drives Migration of Monocytes towards Glioblastoma Cells
Authors: Aretz Philippe , Maciaczyk Donata , Yusuf Suad , Sorg Rüdiger V. , Hänggi Daniel , Liu Hongjia , Liu Hongde , Dakal Tikam Chand , Sharma Amit , Bethanabatla Ramakrishna , Neumann Silke , Maciaczyk Jarek
Published: April 2022

Isocitrate dehydrogenase (IDH)-wildtype glioblastoma (GBM) is a fast growing and highly heterogeneous tumor, often characterized by the presence of glioblastoma stem cells (GSCs). The plasticity of GSCs results in therapy resistance and impairs anti-tumor immune response by influencing immune cells in the tumor microenvironment (TME). Previously, β-catenin was associated with stemness in GBM as well as with immune escape mechanisms. Here, we investigated the effect of β-catenin on attracting monocytes towards GBM cells. In addition, we evaluated whether CCL2 is involved in β-catenin crosstalk between monocytes and tumor cells. Our analysis revealed that shRNA targeting β-catenin in GBMs reduces monocytes attraction and impacts CCL2 secretion. The addition of recombinant CCL2 restores peripheral blood mononuclear cells (PBMC) migration towards medium (TCM) conditioned by shβ-catenin GBM cells. CCL2 knockdown in GBM cells shows similar effects and reduces monocyte migration to a similar extent as β-catenin knockdown. When investigating the effect of CCL2 on β-catenin activity, we found that CCL2 modulates components of the Wnt/β-catenin pathway and alters the clonogenicity of GBM cells. In addition, the pharmacological β-catenin inhibitor MSAB reduces active β-catenin, downregulates the expression of associated genes and alters CCL2 secretion. Taken together, we showed that β-catenin plays an important role in attracting monocytes towards GBM cells in vitro. We hypothesize that the interactions between β-catenin and CCL2 contribute to maintenance of GSCs via modulating immune cell interaction and promoting GBM growth and recurrence.
Porcine Corneas Incubated at Low Humidity Present Characteristic Features Found in Dry Eye Disease
Authors: Netto Alice Rocha Teixeira , Hurst José , Bartz-Schmidt Karl-Ulrich , Schnichels Sven
Published: April 2022

Dry eye is a multifactorial disease that affects the ocular surface and tear fluid. Current treatment options include lubricant eye drop application several times a day. However, these eye drops often cause local side effects like ocular allergies or blurred vision after the application. To test new treatment options, a robust dry eye model is needed. Here, a porcine ex vivo model was established by means of incubation of porcine corneas in low humidity (LH) and characterized by histological damage evaluation, epithelial thickness and by relevant dry eye markers, such as interleukin 1 beta (IL-1β), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), occludin and galectin-3. In the dry eye model proposed, an increased secretion of IL-1β was observed, as well as an upregulation of NF-κB, occludin and galectin-3 mRNA expression. Moreover, the model presented a higher rate of cell death in comparison to the controls. These effects could be reversed with successful treatment of dexamethasone (dexa) and partially reversed with hyaluronic acid (HA) containing eye drops. Furthermore, medium-molecular-weight HA stimulated an increase in IL-1β in the model proposed. In conclusion, this dry eye model mimics the in vivo condition and hence allows for animal-free testing of novel dry eye treatments.
PTPRD and DCC Are Novel BACE1 Substrates Differentially Expressed in Alzheimer’s Disease: A Data Mining and Bioinformatics Study
Authors: Taylor Hannah A. , Simmons Katie J. , Clavane Eva M. , Trevelyan Christopher J. , Brown Jane M. , Przemyłska Lena , Watt Nicole T. , Matthews Laura C. , Meakin Paul J.
Published: April 2022

The β-site Amyloid precursor protein Cleaving Enzyme 1 (BACE1) is an extensively studied therapeutic target for Alzheimer’s disease (AD), owing to its role in the production of neurotoxic amyloid beta (Aβ) peptides. However, despite numerous BACE1 inhibitors entering clinical trials, none have successfully improved AD pathogenesis, despite effectively lowering Aβ concentrations. This can, in part, be attributed to an incomplete understanding of BACE1, including its physiological functions and substrate specificity. We propose that BACE1 has additional important physiological functions, mediated through substrates still to be identified. Thus, to address this, we computationally analysed a list of 533 BACE1 dependent proteins, identified from the literature, for potential BACE1 substrates, and compared them against proteins differentially expressed in AD. We identified 15 novel BACE1 substrates that were specifically altered in AD. To confirm our analysis, we validated Protein tyrosine phosphatase receptor type D (PTPRD) and Netrin receptor DCC (DCC) using Western blotting. These findings shed light on the BACE1 inhibitor failings and could enable the design of substrate-specific inhibitors to target alternative BACE1 substrates. Furthermore, it gives us a greater understanding of the roles of BACE1 and its dysfunction in AD.