bluuebunny/crossref_metadata_2025 huggingface dataset:
Concurrent sequences of Bernoulli trials
Authors: Kaczkowski Stephen
Published: October 2020

Probability and expectation are two distinct measures, both of which can be used to indicate the likelihood of certain events. However, expectation values, which are often associated with waiting times for success, may, at times, speak more clearly and poignantly about the uncertainty of an event than a theoretical probability. To illustrate the point, suppose the probability of choosing a winning lottery ticket is 2.5 × 10−8. This information may not communicate the unlikely odds of winning as clearly as a statement like, “If five lottery tickets are purchased per day, the expected waiting time for a first win is about 22000 years.”
Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the up-regulation of nuclear factor erythroid 2-related factor 2 and decrease blood pressure in rats
Authors: Zarei Mehrdad , Acharya Pooja , Talahalli Ramaprasad Ravichandra
Published: October 2020

Deep-fried vegetable oils are reused multiple times to save costs, and their chronic consumption may cause organ dysfunction. In this study, we assessed the modulatory effects of lipid-solubles from ginger and turmeric that may migrate to oils during heating, on the cardio-hepatic antioxidant defence response and blood pressure in rats. Male Wistar rats were fed with: (1) control (native rapeseed (N-CNO) or native sunflower (N-SFO)) oil, (2) heated (heated rapeseed (H-CNO) or heated sunflower (H-SFO)) oil and (3) heated oil with ginger or turmeric (heated rapeseed oil with ginger (H-CNO + GI) or heated rapeseed oil with turmeric (H-CNO + TU), heated sunflower oil with ginger (H-SFO + GI) or heated sunflower oil with turmeric (H-SFO + TU)) for 120 d. Oxidative stress (OS) markers, antioxidant enzymes, nitric oxide synthase-2 (NOS-2), intercellular adhesion molecule-1 (ICAM-1), nuclear factor erythroid 2-related factor 2 (NRF-2), markers of hepatic and cardiac function and blood pressure were assessed. Feeding heated oils (H-CNO or H-SFO) (1) increased OS markers, NOS-2 and ICAM-1 expression; (2) decreased antioxidant enzyme activity and NRF-2 level; (3) increased marker enzymes of hepatic and cardiac function and (4) increased systolic and diastolic blood pressure significantly (P < 0·05), when compared with respective native oils (N-CNO or N-SFO). However, feeding oils heated with ginger or turmeric positively countered the changes induced by heated oils. Consumption of repeatedly heated oil causes cardio-hepatic dysfunction by inducing OS through NRF-2 down-regulation. Lipid-solubles from ginger and turmeric that may migrate to oil during heating prevent the oxidative stress and blood pressure triggered by heated oils in rats.
Lessons in “Bad Love”: Film Noir and the Rise of the American Oil Regime in Edgar G. Ulmer's Detour (1945)
Authors: PANGBORN MATTHEW
Published: October 2020

This article examines Edgar G. Ulmer's Detour (1945) as an example of film noir's exploration of the affective dimension of early oil-regime America. Drawing on the work of energy-humanities scholars, the article finds the film, and by extension the genre, providing a much-needed ground-level perspective on the efforts of industry and government to stimulate oil consumption by creating desires in a public struggling with the inherent paradoxes of new technologies, foremost among them the car. The automobile gave rise to “automobility,” seemingly an expansion of democratic freedoms, yet that new way of life also entrapped its participants within destructive habits of consumption involving an entire suite of beliefs, practices, habits, and other technologies. These features of the new life, in turn, were understood within a racialized narrative of whiteness to be productive rather than extractive habits. The shadowy and fated network to which film noir gestures, the article thus argues, is not some abstract metaphysical contemplation or generalized conclusion on a period of war, but a felt recognition of the ways the rapidly expanding network of extraction, distribution, and consumption was compelling Americans to remake their lives in dramatic ways that felt beyond their control.
Magnetic energy dissipation during the current quench of disruption in EAST
Authors: Tang T. , Zeng L. , Chen D. L. , Granetz R. S. , Mao S. T. , Duan Y. M. , Zhang L. , Zhuang H. D. , Zhu X. , Liu H. Q. , Shen B. , Jie Y. X. , Gao X.
Published: October 2020

A disruption database characterizing the current quench of disruptions with ITER-like tungsten divertor has been developed on EAST. It provides a large number of plasma parameters describing the predisruptive plasma, current quench time, eddy current, and mitigation by massive impurity injection, which shows that the current quench time strongly depends on magnetic energy and post-disruption electron temperature. Further, the energy balance and magnetic energy dissipation during the current quench phase has been well analysed. Magnetic energy is also demonstrated to be dissipated mainly by ohmic reheating and inductive coupling, and both of the two channels have great effects on current quench time. Also, massive gas injection is an efficient method to speed up the current quench and increase the fraction of impurity radiation.
Epigraphy and Ambition: Building Inscriptions in the Hinterland of Carthage
Authors: Hellström Monica
Published: October 2020

abstract

Building inscriptions are not a good proxy for building activity or, by extension, prosperity. In the part of Roman North Africa where they are the most common, the majority of surviving building inscriptions document the construction of religious buildings by holders of local priesthoods, usually of the imperial cult. The rise of such texts in the second centurya.d., and their demise in the early third century, have no parallel in the epigraphic evidence for other types of construction, and should not be used as evidence for the pace of construction overall. Rather than economic change, these developments reflect shifts in the prospects of aspirational local elites, for whom priesthoods served as springboards to more prestigious positions. These positions were linked to Carthage through administrative arrangements that made this city the metropolis for scores of dependent towns and their ambitious elites.
Is knowing the constructions enough to understand modality patterns in English?
Authors: Fong Ronald
Published: October 2020

Grammarians and teachers would admit that modality is one of the most difficult areas to deal with in English grammar, and it is particularly difficult for learners of English to master this area of grammar. Modality can be achieved by different means (see, for example, Huddleston & Pullum, 2002; Lyons, 1977; Quirk et al., 1985). The following examples illustrate modality by the use of words of various categories:

(1)

Maybe you are right.

(2)

You may be right.

(3)

I think you are right.

(4)

I am certain you are right.
Health Care Services in Shopping Centers: A Routine Mass-Gathering Event
Authors: Ceyhan Mehmet Ali , Demir Gültekin Günhan
Published: October 2020

Background:

Shopping centers (SCs) are social areas with a group of commercial establishments which attract customers of numerous people every day. However, analysis of urgent health conditions and provided health care in SCs has not been performed so far.

Objective:

The aim of the study was to perform a comparative analysis of clients visiting SCs and demographics, complaints, and health care of patients admitted to Emergency Medical Intervention Units (EMIU) located in grand SCs in Ankara, Turkey.

Methods:

Customer and health care records of nine grand SCs in Ankara from January 1, 2018 through December 31, 2018 were evaluated retrospectively. Health care services in EMIUs of SCs were provided by employed medical staff. Data including demographic characteristics, complaints, treatment protocols, discharge, and referral to hospital of the patients were retrospectively analyzed from medical registration forms.

Results:

Medical records of nine grand SCs were analyzed. Number of customers could not be obtained in three SCs due to privacy issues and were not included in patient presentation rate (PPR) and transport-to-hospital rate (TTHR) calculation. Total number of customers in the remaining six SCs were 53,277,239. The total number of patients seeking medical care was 6,749. The number of patients seeking health care in six SCs with known number of customers was 4,498 and PPR ranged from 0.018 to 0.381 patients per 1,000 attendants. The median age of the recorded 4,065 patients (60.2%) was 28 (interquartile range [IQR]: 38-21), and 3,611 (53.5%) of the patients admitted to EMIUs were female. The number of patients treated in the SC was 4,634 (68.6%) and 189 patients (2.8%) were transferred-to-hospital by ambulance for further evaluation and treatment. Transportation to hospital was required in 125 patients who sought medical care in six SCs which provided total number of customers, and TTHR ranged from 0.000 to 0.005 patients per 1,000 attendants. No sudden cardiac death was seen. Medical conditions were the primary reasons for seeking health care. The most frequent causes of presentation were laceration and abrasions (639 patients, 9.4%).

Conclusion:

The PPR and TTHR in SCs are low. The most common causes of presentation are minor conditions and injuries. Majority of urgent medical conditions in SCs can be managed by health care providers in EMIUs.
The Murder of the Jewish Chieftain Ka‘b b. al-Ashraf: A Re-examination
Authors: ROOHI EHSAN
Published: October 2020

The murder of the Prophet's chief Jewish opponent, Ka‘b b. al-Ashraf, led to grave consequences for the tribe of Banū al-Naḍīr and for the Jews as a whole. The incident ushered in a series of hostile Muslim-Jewish encounters that reached its climax in the battle of Khaybar. Despite the constructive study undertaken by previous scholars, there still seem to be some contradictory elements and vague accounts that have been either utterly ignored or for which a satisfactory explanation is lacking. In the light of certain striking pieces of evidence, scattered in unlikely places in the sīra and tafsīr compendia, the present study sets out to examine critically the extent to which the accounts of Ka‘b's murder can be trusted. It will be argued that what we are faced with is seriously distorted material with logical absurdities and discrepancies that cannot easily be reconciled. Apart from the historical reconstruction, special attention will be devoted to a momentous historiographical point—that our reports have been doctored for political reasons. This helps us adopt a more realistic view of the individuals whose names occurred in the accounts of the event in question.
Art Music and Transterritoriality: Reflections on Cuban Migrations to Europe during the 1990s
Authors: MORALES FLORES IVÁN CÉSAR , KUSS MALENA
Published: October 2020

The fall of Communist rule in Eastern Europe (1989–91) had a profound impact on economic, socio-political, and cultural conditions in Cuba during the 1990s. It was the beginning of the so-called ‘Special Period in Times of Peace’. Increasing dire economic conditions on the island led to many changes that impoverished cultural life, including the relocation of composers in Europe, the United States, and other countries in the Americas. The situations encountered by composers who migrated to Europe varied, depending on generational differences and on the dynamics of the socio-political and cultural powers to which each of them were exposed in their new reception contexts. Whether adopting an uncontested identification with the new cultural milieu or resorting to discursive strategies of appropriation and de/re-territorialized negotiation, this text centres on the compositional journeys of four Cuban composers who settled in different European cities: Leo Brouwer (Córdoba, Spain); Eduardo Morales-Caso (Madrid); Keyla Orozco (Amsterdam); and Louis Aguirre (Aalborg, Denmark). Each case represents an invaluable experience of aesthetic reconstruction in a new creative territory that, by its own transterritoriality, has in turn enriched the sonic map of the Caribbean island.
Health Beliefs, Health Anxiety, and Diagnostic Type in Food Hypersensitivity in Adults
Authors: Goble Melissa , Eberhardt Judith , van Schaik Paul
Published: October 2020

Abstract. Background: Food hypersensitivity is often self-diagnosed, and research into barriers to help-seeking is scarce. Aims: This study in the United Kingdom sought to establish the relationship between health beliefs, health anxiety, and diagnostic type (medically diagnosed vs. self-diagnosed) in individuals with food hypersensitivity, and qualitatively explored attitudes of self-diagnosed individuals and their barriers to attaining a medical diagnosis. Method: A mixed-methods design involving 107 participants with food hypersensitivity (64 medically diagnosed and 43 self-diagnosed). Participants completed an adapted version of the health belief model questionnaire and a health anxiety questionnaire. A subset of six self-diagnosed participants took part in semi-structured interviews. Results: Binary logistic regression showed that health anxiety, perceived susceptibility, and perceived severity were significantly associated with diagnostic type. Qualitative thematic analysis of interviews yielded three themes: control over food, diagnosis, and treatment; judgment regarding feeling judged negatively on one’s choice of food, and being compared to fad-dieters; and the public’s and participants’ own lack of perceived severity of food hypersensitivity. Limitation: The sample was self-selected and therefore not necessarily representative of the population; however, an adult population was examined in an area that has so far largely studied children. Conclusion: Health psychologists should become involved in developing and testing interventions to help those with food hypersensitivity to control and reduce distress. Further researching the issues of control, judgment, and perceived severity could help tackle barriers to help-seeking behavior.
A New Type of Polar Cap Arc Observed in the ~1500 MLT Sector: 1. Northern Hemisphere Observations
Authors: Han De‐Sheng , Feng Hui‐Ting , Zhang Hong , Zhou Su , Zhang Yongliang
Published: October 2020

A new type of polar cap aurora observed in ~1500 MLT sector, abbreviated as 15MLT‐PCA, is identified on examining the long‐term spectrographic imager observations on the DMSP satellite over the Northern Hemisphere. Apparent dependencies of 15MLT‐PCA on the solar cycle, season, universal time, and interplanetary magnetic field (IMF) are presented, which indicate that the 15MLT‐PCA occurs in the northern summer under conditions of large dipole tilt angle, long‐lasting positive IMF By, and predominant negative Bx. The IMF Bz and other solar wind conditions show less control over the occurrence of 15MLT‐PCA. The particle precipitations of 15MLT‐PCA show magnetosheath‐like or mantle‐like properties with the ions showing clear energy dispersion property. We suggest that the 15MLT‐PCA is caused by reconnection between a twisted lobe field line and an antisunward IMF field line, which differs from the mechanism for High‐latitude dayside aurora (HiLDA) that share some common observational features with the 15MLT‐PCA.
The hierarchical assembly of septins revealed by high-speed AFM
Authors: Jiao Fang , Cannon Kevin S. , Lin Yi-Chih , Gladfelter Amy S. , Scheuring Simon
Published: October 2020

Septins are GTP-binding proteins involved in diverse cellular processes including division and membrane remodeling. Septins form linear, palindromic heteromeric complexes that can assemble in filaments and higher-order structures. Structural studies revealed various septin architectures, but questions concerning assembly-dynamics and -pathways persist. Here we used high-speed atomic force microscopy (HS-AFM) and kinetic modeling which allowed us to determine that septin filament assembly was a diffusion-driven process, while formation of higher-order structures was complex and involved self-templating. Slightly acidic pH and increased monovalent ion concentrations favor filament-assembly, -alignment and -pairing. Filament-alignment and -pairing further favored diffusion-driven assembly. Pairing is mediated by the septin N-termini face, and may occur symmetrically or staggered, likely important for the formation of higher-order structures of different shapes. Multilayered structures are templated by the morphology of the underlying layers. The septin C-termini face, namely the C-terminal extension of Cdc12, may be involved in membrane binding.
Global distribution and conservation status of ecologically rare mammal and bird species
Authors: Loiseau Nicolas , Mouquet Nicolas , Casajus Nicolas , Grenié Matthias , Guéguen Maya , Maitner Brian , Mouillot David , Ostling Annette , Renaud Julien , Tucker Caroline , Velez Laure , Thuiller Wilfried , Violle Cyrille
Published: October 2020

Identifying species that are both geographically restricted and functionally distinct, i.e. supporting rare traits and functions, is of prime importance given their risk of extinction and their potential contribution to ecosystem functioning. We use global species distributions and functional traits for birds and mammals to identify the ecologically rare species, understand their characteristics, and identify hotspots. We find that ecologically rare species are disproportionately represented in IUCN threatened categories, insufficiently covered by protected areas, and for some of them sensitive to current and future threats. While they are more abundant overall in countries with a low human development index, some countries with high human development index are also hotspots of ecological rarity, suggesting transboundary responsibility for their conservation. Altogether, these results state that more conservation emphasis should be given to ecological rarity given future environmental conditions and the need to sustain multiple ecosystem processes in the long-term.
Genome-wide chromatin accessibility is restricted by ANP32E
Authors: Murphy Kristin E. , Meng Fanju W. , Makowski Claire E. , Murphy Patrick J.
Published: October 2020

Genome-wide chromatin state underlies gene expression potential and cellular function. Epigenetic features and nucleosome positioning contribute to the accessibility of DNA, but widespread regulators of chromatin state are largely unknown. Our study investigates how coordination of ANP32E and H2A.Z contributes to genome-wide chromatin state in mouse fibroblasts. We define H2A.Z as a universal chromatin accessibility factor, and demonstrate that ANP32E antagonizes H2A.Z accumulation to restrict chromatin accessibility genome-wide. In the absence of ANP32E, H2A.Z accumulates at promoters in a hierarchical manner. H2A.Z initially localizes downstream of the transcription start site, and if H2A.Z is already present downstream, additional H2A.Z accumulates upstream. This hierarchical H2A.Z accumulation coincides with improved nucleosome positioning, heightened transcription factor binding, and increased expression of neighboring genes. Thus, ANP32E dramatically influences genome-wide chromatin accessibility through subtle refinement of H2A.Z patterns, providing a means to reprogram chromatin state and to hone gene expression levels.
Structural characterization of the ICOS/ICOS-L immune complex reveals high molecular mimicry by therapeutic antibodies
Authors: Rujas Edurne , Cui Hong , Sicard Taylor , Semesi Anthony , Julien Jean-Philippe
Published: October 2020

The inducible co-stimulator (ICOS) is a member of the CD28/B7 superfamily, and delivers a positive co-stimulatory signal to activated T cells upon binding to its ligand (ICOS-L). Dysregulation of this pathway has been implicated in autoimmune diseases and cancer, and is currently under clinical investigation as an immune checkpoint blockade. Here, we describe the molecular interactions of the ICOS/ICOS-L immune complex at 3.3 Å resolution. A central FDPPPF motif and residues within the CC’ loop of ICOS are responsible for the specificity of the interaction with ICOS-L, with a distinct receptor binding orientation in comparison to other family members. Furthermore, our structure and binding data reveal that the ICOS N110 N-linked glycan participates in ICOS-L binding. In addition, we report crystal structures of ICOS and ICOS-L in complex with monoclonal antibodies under clinical evaluation in immunotherapy. Strikingly, antibody paratopes closely mimic receptor-ligand binding core interactions, in addition to contacting peripheral residues to confer high binding affinities. Our results uncover key molecular interactions of an immune complex central to human adaptive immunity and have direct implications for the ongoing development of therapeutic interventions targeting immune checkpoint receptors.
CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholerae
Authors: Weaver Sara J. , Ortega Davi R. , Sazinsky Matthew H. , Dalia Triana N. , Dalia Ankur B. , Jensen Grant J.
Published: October 2020

Natural transformation is the process by which bacteria take up genetic material from their environment and integrate it into their genome by homologous recombination. It represents one mode of horizontal gene transfer and contributes to the spread of traits like antibiotic resistance. InVibrio cholerae, a type IVa pilus (T4aP) is thought to facilitate natural transformation by extending from the cell surface, binding to exogenous DNA, and retracting to thread this DNA through the outer membrane secretin, PilQ. Here, we use a functional tagged allele of VcPilQ purified from nativeV. choleraecells to determine the cryoEM structure of the VcPilQ secretin in amphipol to ~2.7 Å. We use bioinformatics to examine the domain architecture and gene neighborhood of T4aP secretins in Proteobacteria in comparison with VcPilQ. This structure highlights differences in the architecture of the T4aP secretin from the type II and type III secretion system secretins. Based on our cryoEM structure, we design a series of mutants to reversibly regulate VcPilQ gate dynamics. These experiments support the idea of VcPilQ as a potential druggable target and provide insight into the channel that DNA likely traverses to promote the spread of antibiotic resistance via horizontal gene transfer by natural transformation.
Preparation of nickel-iron hydroxides by microorganism corrosion for efficient oxygen evolution
Authors: Yang Huan , Gong Lanqian , Wang Hongming , Dong Chungli , Wang Junlei , Qi Kai , Liu Hongfang , Guo Xingpeng , Xia Bao Yu
Published: October 2020

Nickel–iron composites are efficient in catalyzing oxygen evolution. Here, we develop a microorganism corrosion approach to construct nickel–iron hydroxides. The anaerobic sulfate-reducing bacteria, using sulfate as the electron acceptor, play a significant role in the formation of iron sulfide decorated nickel–iron hydroxides, which exhibit excellent electrocatalytic performance for oxygen evolution. Experimental and theoretical investigations suggest that the synergistic effect between oxyhydroxides and sulfide species accounts for the high activity. This microorganism corrosion strategy not only provides efficient candidate electrocatalysts but also bridges traditional corrosion engineering and emerging electrochemical energy technologies.
JMJD3 acts in tandem with KLF4 to facilitate reprogramming to pluripotency
Authors: Huang Yinghua , Zhang Hui , Wang Lulu , Tang Chuanqing , Qin Xiaogan , Wu Xinyu , Pan Meifang , Tang Yujia , Yang Zhongzhou , Babarinde Isaac A. , Lin Runxia , Ji Guanyu , Lai Yiwei , Xu Xueting , Su Jianbin , Wen Xue , Satoh Takashi , Ahmed Tanveer , Malik Vikas , Ward Carl , Volpe Giacomo , Guo Lin , Chen Jinlong , Sun Li , Li Yingying , Huang Xiaofen , Bao Xichen , Gao Fei , Liu Baohua , Zheng Hui , Jauch Ralf , Lai Liangxue , Pan Guangjin , Chen Jiekai , Testa Giuseppe , Akira Shizuo , Hu Jifan , Pei Duanqing , Hutchins Andrew P. , Esteban Miguel A. , Qin Baoming
Published: October 2020

The interplay between the Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) and transcriptional/epigenetic co-regulators in somatic cell reprogramming is incompletely understood. Here, we demonstrate that the histone H3 lysine 27 trimethylation (H3K27me3) demethylase JMJD3 plays conflicting roles in mouse reprogramming. On one side, JMJD3 induces the pro-senescence factor Ink4a and degrades the pluripotency regulator PHF20 in a reprogramming factor-independent manner. On the other side, JMJD3 is specifically recruited by KLF4 to reduce H3K27me3 at both enhancers and promoters of epithelial and pluripotency genes. JMJD3 also promotes enhancer-promoter looping through the cohesin loading factor NIPBL and ultimately transcriptional elongation. This competition of forces can be shifted towards improved reprogramming by using early passage fibroblasts or boosting JMJD3’s catalytic activity with vitamin C. Our work, thus, establishes a multifaceted role for JMJD3, placing it as a key partner of KLF4 and a scaffold that assists chromatin interactions and activates gene transcription.
High-throughput prediction of the carrier relaxation time via data-driven descriptor
Authors: Zhou Zizhen , Cao Guohua , Liu Jianghui , Liu Huijun
Published: October 2020

It has been demonstrated that many promising thermoelectric materials, such as tetradymite compounds are also three-dimensional topological insulators. In both cases, a fundamental question is the evaluation of carrier relaxation time, which is usually a rough task due to the complicated scattering mechanisms. Previous works using the simple deformation potential theory or considering complete electron-phonon coupling are, however, restricted to small systems. By adopting a data-driven method named SISSO (Sure Independence Screening and Sparsifying Operator) with the training data obtained via deformation potential theory, we propose an efficient and physically interpretable descriptor to evaluate the relaxation time, using tetradymites as prototypical examples. Without any input from first-principles calculations, the descriptor contains only several elemental properties of the constituent atoms, and could be utilized to quickly and reliably predict the carrier relaxation time of a substantial number of tetradymites with arbitrary stoichiometry.
Quantitative magnetic resonance image assessment of the optic nerve and surrounding sheath after spaceflight
Authors: Rohr Jesse J. , Sater Stuart , Sass Austin M. , Marshall-Goebel Karina , Ploutz-Snyder Robert J. , Ethier C. Ross , Stenger Michael B. , Martin Bryn A. , Macias Brandon R.
Published: October 2020

A subset of long-duration spaceflight astronauts have experienced ophthalmic abnormalities, collectively termed spaceflight-associated neuro-ocular syndrome (SANS). Little is understood about the pathophysiology of SANS; however, microgravity-induced alterations in intracranial pressure (ICP) due to headward fluid shifts is the primary hypothesized contributor. In particular, potential changes in optic nerve (ON) tortuosity and ON sheath (ONS) distension may indicate altered cerebrospinal fluid dynamics during weightlessness. The present longitudinal study aims to provide a quantitative analysis of ON and ONS cross-sectional areas, and ON deviation, an indication of tortuosity, before and after spaceflight. Ten astronauts undergoing ~6-month missions on the International Space Station (ISS) underwent high-resolution magnetic resonance imaging (MRI) preflight and at five recovery time points extending to 1 year after return from the ISS. The mean changes in ON deviation, ON cross-sectional area, and ONS cross-sectional area immediately post flight were −0.14 mm (95% CI: −0.36 to 0.08, Bonferroni-adjusted P = 1.00), 0.13 mm2 (95% CI −0.66 to 0.91, Bonferroni-adjusted P = 1.00), and −0.22 mm2 (95% CI: −1.78 to 1.34, Bonferroni-adjusted P = 1.00), respectively, and remained consistent during the recovery period. Terrestrially, ONS distension is associated with increased ICP; therefore, these results suggest that, on average, ICP was not pathologically elevated immediately after spaceflight. However, a subject diagnosed with optic disc edema (Frisen Grade 1, right eye) displayed increased ONS area post flight, although this increase is relatively small compared to clinical populations with increased ICP. Advanced quantitative MRI-based assessment of the ON and ONS could help our understanding of SANS and the role of ICP.
The first versatile human iPSC-based model of ectopic virus induction allows new insights in RNA-virus disease
Authors: Peischard Stefan , Ho Huyen Tran , Piccini Ilaria , Strutz-Seebohm Nathalie , Röpke Albrecht , Liashkovich Ivan , Gosain Hiteshika , Rieger Bettina , Klingel Karin , Eggers Britta , Marcus Katrin , Linke Wolfgang A. , Müller Frank Ulrich , Ludwig Stephan , Greber Boris , Busch Karin , Seebohm Guiscard
Published: October 2020

A detailed description of pathophysiological effects that viruses exert on their host is still challenging. For the first time, we report a highly controllable viral expression model based on an iPS-cell line from a healthy human donor. The established viral model system enables a dose-dependent and highly localized RNA-virus expression in a fully controllable environment, giving rise for new applications for the scientific community.
An agent-based algorithm resembles behaviour of tree-dwelling bats under fission–fusion dynamics
Authors: Zelenka Ján , Kasanický Tomáš , Budinská Ivana , Kaňuch Peter
Published: October 2020

Utilization of computational approach in the study of social behaviour of animals is increasing and we attempted such an approach in our study of tree-dwelling bats. These bats live in highly dynamic fission–fusion societies that share multiple roosts in a common home range. The key behavioural component associated with complex and non-centralized decision-making processes in roost switching is swarming around potential locations in order to recruit members to the new roost. To understand roost switching dynamics of bat groups in their natural environment, we employed a computational model, the SkyBat, which is based on swarm algorithm, to model this process. In a simulated environment of this agent-based model, we replicated natural fission–fusion dynamics of the Leisler’s bat,Nyctalus leisleri, groups according to predefined species and habitat parameters. Spatiotemporal patterns of swarming activity of agents were similar to bats. The number of simulated groups formed prior to sunrise, the mean number of individuals in groups and the roost height did not differ significantly from data on a local population of bats collected in the field. Thus, the swarm algorithm gave a basic framework of roost-switching, suggesting possible applications in the study of bat behaviour in rapidly changing environments as well as in the field of computer science.
Reduced spermatozoa functionality during stress is the consequence of adrenergic-mediated disturbance of mitochondrial dynamics markers
Authors: Starovlah Isidora M. , Radovic Pletikosic Sava M. , Kostic Tatjana S. , Andric Silvana A.
Published: October 2020

Here we investigate the stress-signaling responsible for the effects of acute/repeated psychological stresses (the most common stresses in human society) on spermatozoa number and functionality, as well as the transcriptional profile of mitochondrial dynamics markers by using the in vivo and ex vivo approaches. Acute and repeated stress inhibit spermatozoa functionality (acute –> 3.2-fold, repeated –> 2.5-fold), while only repeated stress reduces the spermatozoa number (1.7-fold). Stress hormones mimic these effects and decrease the spermatozoa functionality (adrenaline: 10 µM –> 2.4-fold, 100 µM – > 2.8-fold; hydrocortisone: 50 pM –> 2.7-fold, 500 pM –> 8.5-fold). They also significantly disturb the transcriptional profile of all main mitochondrial dynamics markers in spermatozoa. Ex vivo manipulation of stress signaling in spermatozoa reveals that most of these effects are mediated through ɑ1-and/or-β-adrenergic receptors. The transcription of these receptors and their kinases in the same samples is under the significant influence of adrenergic signaling. Our results are the first to show the importance of mitochondrial dynamics markers in spermatozoa since the transcriptional profiles of sixteen-out-of-ninteen are disturbed by manipulation of stress-hormones-signaling. This is a completely new molecular approach to assess spermatozoa functionality and it is important for a better understanding of the correlations between stress, environmental-life-style and other factors, and male (in)fertility.
Spectral weight reduction of two-dimensional electron gases at oxide surfaces across the ferroelectric transition
Authors: Jaiban P. , Lu M.-H. , Eknapakul T. , Chaiyachad S. , Yao S. H. , Pisitpipathsin N. , Unruan M. , Siriroj S. , He R.-H. , Mo S.-K. , Watcharapasorn A. , Yimnirun R. , Tokura Y. , Shen Z.-X. , Hwang H. Y. , Maensiri S. , Meevasana W.
Published: October 2020

The discovery of a two-dimensional electron gas (2DEG) at the $$\hbox {LaAlO}_3/\hbox {SrTiO}_3$$

LaAlO
3

/

SrTiO
3

interface has set a new platform for all-oxide electronics which could potentially exhibit the interplay among charge, spin, orbital, superconductivity, ferromagnetism and ferroelectricity. In this work, by using angle-resolved photoemission spectroscopy and conductivity measurement, we found the reduction of 2DEGs and the changes of the conductivity nature of some ferroelectric oxides including insulating Nb-lightly-substituted $$\hbox {KTaO}_3$$

KTaO
3

, $$\hbox {BaTiO}_3$$

BaTiO
3

(BTO) and (Ca,Zr)-doped BTO across paraelectric-ferroelectric transition. We propose that these behaviours could be due to the increase of space-charge screening potential at the 2DEG/ferroelectric regions which is a result of the realignment of ferroelectric polarisation upon light irradiation. This finding suggests an opportunity for controlling the 2DEG at a bare oxide surface (instead of interfacial system) by using both light and ferroelectricity.
Rewiring of gene expression in circulating white blood cells is associated with pregnancy outcome in heifers (Bos taurus)
Authors: Moorey Sarah E. , Walker Bailey N. , Elmore Michelle F. , Elmore Joshua B. , Rodning Soren P. , Biase Fernando H.
Published: October 2020

Infertility is a challenging phenomenon in cattle that reduces the sustainability of beef production worldwide. Here, we tested the hypothesis that gene expression profiles of protein-coding genes expressed in peripheral white blood cells (PWBCs), and circulating micro RNAs in plasma, are associated with female fertility, measured by pregnancy outcome. We drew blood samples from 17 heifers on the day of artificial insemination and analyzed transcript abundance for 10,496 genes in PWBCs and 290 circulating micro RNAs. The females were later classified as pregnant to artificial insemination, pregnant to natural breeding or not pregnant. We identified 1860 genes producing significant differential coexpression (eFDR < 0.002) based on pregnancy outcome. Additionally, 237 micro RNAs and 2274 genes in PWBCs presented differential coexpression based on pregnancy outcome. Furthermore, using a machine learning prediction algorithm we detected a subset of genes whose abundance could be used for blind categorization of pregnancy outcome. Our results provide strong evidence that transcript abundance in circulating white blood cells is associated with fertility in heifers.
Transcriptome sequencing and histology reveal dosage compensation in the liver of triploid pre-smolt Atlantic salmon
Authors: Odei Derrick K. , Hagen Ørjan , Peruzzi Stefano , Falk-Petersen Inger-Britt , Fernandes Jorge M. O.
Published: October 2020

Triploid Atlantic salmon (Salmo salar L.) is seen as one of the best solutions to solve key issues in the salmon farming industry, such as the impact of escapees on wild stocks and pre-harvest sexual maturation. However, the effects of triploidy on salmon smoltification are poorly understood at the molecular level, even though smoltification is a very sensitive period that has a major influence on survival rate and performance of farmed salmon. In this study, we have compared the liver transcriptomes of diploid and triploid Atlantic salmon at three ontogeny stages: fry, parr and smolt. In diploid fish, a total of 2,655 genes were differentially expressed between fry and parr, whereas 506 genes had significantly different transcript levels between parr and smolts. In triploids, 1,507 and 974 genes were differentially expressed between fry and parr, and between parr and smolts, respectively. Most of these genes were down-regulated and 34 genes were differentially expressed between ploidies at the same stage. In both ploidy groups, the top differentially expressed genes with ontogeny stage belonged to common functional categories that can be related to smoltification. Nucleotide and energy metabolism were significantly down-regulated in fry when compared to parr, while immune system processes were significantly down-regulated in parr when compared to smolts. The close resemblance of enriched biological processes and pathways between ploidy groups suggests that triploidy is regulated by genome dosage compensation in Atlantic salmon. Histological analysis revealed that areas of vacuolization (steatosis) were present only in fry and parr stages, in contrast to a compact cellular histology with glycogen granules after smoltification. There was no significant difference in vacuolization between ploidy groups at the fry stage but the liver of diploid parr had a 33.5% higher vacuolization area compared to their triploid counterparts. Taken together, our data provide novel insights into the changes that occur at the molecular and histological level in the liver of both diploid and triploid Atlantic salmon prior to and during smoltification.
Modeling the potential of wAu-Wolbachia strain invasion in mosquitoes to control Aedes-borne arboviral infections
Authors: Ogunlade Samson T. , Adekunle Adeshina I. , Meehan Michael T. , Rojas Diana P. , McBryde Emma S.
Published: October 2020

Arboviral infections such as dengue, Zika and chikungunya are fast spreading diseases that pose significant health problems globally. In order to control these infections, an intracellular bacterium called Wolbachia has been introduced into wild-type mosquito populations in the hopes of replacing the vector transmitting agent, Aedes aegypti with one that is incapable of transmission. In this study, we developed a Wolbachia transmission model for the novel wAu strain which possesses several favourable traits (e.g., enhanced viral blockage and maintenance at higher temperature) but not cyctoplasmic incompatibility (CI)—when a Wolbachia-infected male mosquito mates with an uninfected female mosquito, producing no viable offspring. This model describes the competitive dynamics between wAu-Wolbachia-infected and uninfected mosquitoes and the role of imperfect maternal transmission. By analysing the system via computing the basic reproduction number(s) and stability properties, the potential of the wAu strain as a viable strategy to control arboviral infections is established. The results of this work show that enhanced maintenance of Wolbachia infection at higher temperatures can overcome the lack of CI induction to support wAu-Wolbachia infected mosquito invasion. This study will support future arboviral control programs, that rely on the introduction of new Wolbachia variants.
Hot spot profiles of SARS-CoV-2 and human ACE2 receptor protein protein interaction obtained by density functional tight binding fragment molecular orbital method
Authors: Lim Hocheol , Baek Ayoung , Kim Jongwan , Kim Min Sung , Liu Jiaxin , Nam Ky-Youb , Yoon JeongHyeok , No Kyoung Tai
Published: October 2020

The prevalence of a novel β-coronavirus (SARS-CoV-2) was declared as a public health emergency of international concern on 30 January 2020 and a global pandemic on 11 March 2020 by WHO. The spike glycoprotein of SARS-CoV-2 is regarded as a key target for the development of vaccines and therapeutic antibodies. In order to develop anti-viral therapeutics for SARS-CoV-2, it is crucial to find amino acid pairs that strongly attract each other at the interface of the spike glycoprotein and the human angiotensin-converting enzyme 2 (hACE2) complex. In order to find hot spot residues, the strongly attracting amino acid pairs at the protein–protein interaction (PPI) interface, we introduce a reliable inter-residue interaction energy calculation method, FMO-DFTB3/D/PCM/3D-SPIEs. In addition to the SARS-CoV-2 spike glycoprotein/hACE2 complex, the hot spot residues of SARS-CoV-1 spike glycoprotein/hACE2 complex, SARS-CoV-1 spike glycoprotein/antibody complex, and HCoV-NL63 spike glycoprotein/hACE2 complex were obtained using the same FMO method. Following this, a 3D-SPIEs-based interaction map was constructed with hot spot residues for the hACE2/SARS-CoV-1 spike glycoprotein, hACE2/HCoV-NL63 spike glycoprotein, and hACE2/SARS-CoV-2 spike glycoprotein complexes. Finally, the three 3D-SPIEs-based interaction maps were combined and analyzed to find the consensus hot spots among the three complexes. As a result of the analysis, two hot spots were identified between hACE2 and the three spike proteins. In particular, E37, K353, G354, and D355 of the hACE2 receptor strongly interact with the spike proteins of coronaviruses. The 3D-SPIEs-based map would provide valuable information to develop anti-viral therapeutics that inhibit PPIs between the spike protein of SARS-CoV-2 and hACE2.
Carotenoid metabolite and transcriptome dynamics underlying flower color in marigold (Tagetes erecta L.)
Authors: Zhang Huali , Zhang Shiya , Zhang Hua , Chen Xi , Liang Fang , Qin Helan , Zhang Yue , Cong Richen , Xin Haibo , Zhang Zhao
Published: October 2020

Marigold (Tagetes erecta L.) is an important ornamental plant with a wide variety of flower colors. Despite its economic value, few biochemical and molecular studies have explored the generation of flower color in this species. To study the mechanism underlying marigold petal color, we performed a metabolite analysis and de novo cDNA sequencing on the inbred line ‘V-01’ and its petal color mutant ‘V-01M’ at four flower developmental stages. A total of 49,217 unigenes were identified from 24 cDNA libraries. Based on our metabolites and transcriptomic analyses, we present an overview of carotenoid biosynthesis, degradation, and accumulation in marigold flowers. The carotenoid content of the yellow mutant ‘V-01M’ was higher than that of the orange inbred line ‘V-01’, and the abundances of the yellow compounds lutein, neoxanthin, violaxanthin, zeaxanthin, and antheraxanthin were significantly higher in the mutant. During flower development, the carotenoid biosynthesis genes were upregulated in both ‘V-01’ and ‘V-01M’, with no significant differences between the two lines. By contrast, the carotenoid degradation genes were dramatically downregulated in the yellow mutant ‘V-01M’. We therefore speculate that the carotenoid degradation genes are the key factors regulating the carotenoid content of marigold flowers. Our research provides a large amount of transcriptomic data and insights into the marigold color metabolome.
Efficacy and safety of newer P2Y12 inhibitors for acute coronary syndrome: a network meta-analysis
Authors: Fei Yue , Lam Cheuk Kiu , Cheung Bernard Man Yung
Published: October 2020

Whether newer P2Y12 inhibitors are more efficacious and safer than clopidogrel and whether there is a superior one remain uncertain. We compared the effect of P2Y12 inhibitors on clinical outcomes in patients with acute coronary syndrome (ACS). Randomized controlled trials comparing clopidogrel, prasugrel, ticagrelor, or cangrelor, in combination with aspirin were searched. Sixteen trials with altogether 77,896 patients were included. Compared to clopidogrel, cardiovascular mortality was reduced with prasugrel (OR 0.85, 95% CI 0.75–0.97) and ticagrelor (0.82, 0.73–0.93). Myocardial infarction (0.75, 0.63–0.89) and major adverse cardiovascular events (0.80, 0.69–0.94) were reduced by prasugrel. Stent thrombosis was reduced by prasugrel (0.49, 0.38–0.63), ticagrelor (0.72, 0.57–0.90), and cangrelor (0.59, 0.43–0.81). It was reduced more by prasugrel than ticagrelor (0.69, 0.51–0.93). There were more major bleeds with prasugrel (1.24, 1.05–1.48). Thrombolysis in Myocardial Infarction (TIMI) major bleeding was increased with prasugrel compared to clopidogrel (1.36, 1.11–1.66) and ticagrelor (1.33, 1.06–1.67). TIMI minor bleeding was increased with prasugrel (1.44, 1.16–1.77) and cangrelor (1.47, 1.01–2.16) compared to clopidogrel while it was increased with prasugrel compared to ticagrelor (1.32, 1.01–1.72). Prasugrel is preferable to those ACS patients at low bleeding risk to reduce cardiovascular events whereas ticagrelor is a relatively safe antiplatelet drug of choice for most patients.
Study of the heavily p-type doping of cubic GaN with Mg
Authors: Hernández-Gutiérrez C. A. , Casallas-Moreno Y. L. , Rangel-Kuoppa Victor-Tapio , Cardona Dagoberto , Hu Yaoqiao , Kudriatsev Yuri , Zambrano-Serrano M. A. , Gallardo-Hernandez S. , Lopez-Lopez M.
Published: October 2020

We have studied the Mg doping of cubic GaN grown by plasma-assisted Molecular Beam Epitaxy (PA-MBE) over GaAs (001) substrates. In particular, we concentrated on conditions to obtain heavy p-type doping to achieve low resistance films which can be used in bipolar devices. We simulated the Mg-doped GaN transport properties by density functional theory (DFT) to compare with the experimental data. Mg-doped GaN cubic epitaxial layers grown under optimized conditions show a free hole carrier concentration with a maximum value of 6 × 1019 cm−3 and mobility of 3 cm2/Vs. Deep level transient spectroscopy shows the presence of a trap with an activation energy of 114 meV presumably associated with nitrogen vacancies, which could be the cause for the observed self-compensation behavior in heavily Mg-doped GaN involving Mg-VN complexes. Furthermore, valence band analysis by X-ray photoelectron spectroscopy and photoluminescence spectroscopy revealed an Mg ionization energy of about 100 meV, which agrees quite well with the value of 99.6 meV obtained by DFT. Our results show that the cubic phase is a suitable alternative to generate a high free hole carrier concentration for GaN.
Fitting the magnetoresponses of the OLED using polaron pair model to obtain spin-pair dynamics and local hyperfine fields
Authors: Weng Zhichao , Gillin William P. , Kreouzis Theo
Published: October 2020

Organic light-emitting diode (OLED) displays a sign reversal magnetic field effect (MFE) when the applied magnetic field range is reduced to the sub-milliTesla range and the Polaron Pair Model has been successful in explaining the ultra-small MFE. Here, we obtained high resolution (~ 1 µT) magnetoconductance (MC) and magnetoelectroluminescence (MEL) of a tris-(8-hydroxyquinoline)aluminium-based (Alq3) OLED within the magnetic field range of ± 500 µT with the earth magnetic field components cancelled. A clear “W” shaped MC with a dip position of ± 250 µT and a monotonic MEL were observed. We demonstrate a fitting technique using the polaron pair model to the experimentally obtained MC and MEL. The fitting process extracts physically significant parameters within a working OLED: the local hyperfine fields for electron and hole in Alq3: Bhf1 = (0.63 ± 0.01) mT (electron), Bhf2 = (0.24 ± 0.01) mT (hole); the separation rates for singlet and triplet polaron pairs: kS,s = (44.59 ± 0.01) MHz, kT,s = (43.97 ± 0.01) MHz, and the recombination rate for singlet polaron pair kS,r = (88 ± 6) MHz. The yielded parameters are highly reproducible across different OLEDs and are in broad agreement with density functional theory (DFT) calculations and reported experimental observations. This demonstrates the feasibility of this fitting technique to approach any working OLED for obtaining significant microscopic parameters.