bluuebunny/crossref_metadata_2025 huggingface dataset:
Основные концепции биологического вида и их функциональный потенциал в палеоантропологии /
THE MAIN BIOLOGICAL SPECIES CONCEPTS AND THEIR FUNCTIONAL POTENTIAL IN PALEOANTHROPOLOGY
Authors: Shulimova E. A.
Published: March 2023

Фрагментарность палеоантропологических находок и неполнота палеоантропологической летописи не позволяют рассмотреть «вид», как функциональную категорию научного изыскания древнейшей истории человечества, основываясь на классическое определение «вида», данное Э. Майером, поскольку исследовать степень репродуктивной изоляции у не живущих видов невозможно. Имеющийся в научном обороте широкий спектр концептуальных подходов определения понятия «вид», позволяет изучить «вид» в рамках палеоантропологии. В статье рассмотрены и проанализированы 22 концепции биологического вида на предмет их функционального потенциала в палеоантропологии. Произведено сопоставление видов рода Homo и семейства австралопитековых. Осуществлен обзор экологических, демографических, миграционных факторов, а также факторов полового отбора и систематических построений. Показаны достижения и перспективы генетических исследований рода Homo в направлении понимания категории «вид», в частности. Выделены три группы концепций: «рабочие», «частично рабочие» и «нерабочие» в палеоантропологии. Для первых двух групп обозначены возможные варианты их применения в исследовании категории «вида» в роду Homo. При выявлении «частично рабочий» концепции вида дано конкретное указание на возможный вариант применения концепции вида в качестве функциональной в палеоантропологии.

The classical definition of “species” formulated by E. Mayr is based on reproductive isolation of a group, which is impossible to study when the group is extinct. Such studies of hominins are further hampered by the fragmentary nature of paleoanthropological fossils and the incompleteness of the paleoanthropological chronicle. But the wide range of conceptual approaches to define the concept of “species” available in scientific publications allows us to study “species” within paleoanthropology as a scientific field. In the article, twenty‑two concepts of a biological species in paleoanthropology are examined for their functional potential. Species of the Homo and Australopithecus genera are compared. An overview of environmental, demographic, migration factors, as well as factors of sexual selection and systematic constructions is given. The article also highlights the achievements and prospects of genetic research of the genus Homo for understanding the category “species”. Three groups of concepts were identified: “operational”, “partly operational” and “non‑operational” in paleoanthropology. For the first two groups, the article shows possible ways of their application to the study of the category of “species” within the Homo genus with a specific example for a “partly operational” concept.
The application of metagenomic next-generation sequencing in patients with infection or colonization caused by Lichtheimia species
Authors: Chen Lihua , Peng Weiting , Lan Gongbin , Long Guo , Yang Honghui , Xu Yajing , Fu Ai , Yi Huimin , Wan Qiquan
Published: March 2023

Background

Mucormycosis is considered the fourth most common invasive fungal disease after candidiasis, aspergillosis and cryptococcosis. Lichtheimia species accounted for 5%-29% of all mucormycosis. However, available data on species-specific analysis of Lichtheimia infections are limited.

Methods

This study included nine patients hospitalized in five hospitals in two cities in south China with mucormycosis or colonization caused by Lichtheimia species, diagnosed mainly by metagenomic next-generation sequencing (mNGS). The corresponding medical records were reviewed, and the clinical data analyzed included demographic characteristics, site of infection, host factors and type of underlying disease, diagnosis, clinical course, management, and prognosis.

Results

In this study, nine patients with Lichtheimia infections or colonization had a recent history of haematological malignancy (33.3%), solid organ transplants (33.3%), pulmonary disease (22.2%), and trauma (11.1%) and were categorized as 11.1% (one case) proven, 66.7% (six cases) probable mucormycosis and 22.2% (two cases) colonization. Pulmonary mucormycosis or colonization was the predominant presentation in 77.8% of cases and mucormycosis caused by Lichtheimia resulted in death in four out of seven patients (57.1%).

Conclusion

These cases highlight the importance of early diagnosis and combined therapy for these sporadic yet life-threatening infections. Further studies on the diagnosis and control of Lichtheimia infection in China are required.
Study on the time-varying fatigue reliability of the invert structure of subway tunnel under the action of train loads
Authors: Chen Zhaofeng , Xu Linrong , Huang Changxi , Wu Lei , Shang Yonghui
Published: March 2023

Introduction: The present study examines the fatigue reliability of subway tunnel invert structures subjected to train-induced loads, a critical factor in ensuring the safety and longevity of these vital infrastructures.

Methods: By integrating higher-order moment theory, fatigue equations, and cumulative damage theory, this research analyzes the impact of train loads on invert structures, taking into account factors such as tension stress, compression stress, train axle weight, speed, and lining thickness.

Results: The findings indicate that the reinforcement of the invert structure is required under tension stress, while it is not necessary for compression stress. Notably, the train’s axle weight and speed exert a significant influence on the fatigue reliability index, with speed displaying a marginally greater effect than axle weight. Conversely, the lining thickness demonstrates a negligible impact on the reliability index. As time progresses, the fatigue reliability index diminishes, resulting in an elevated probability of failure.

Discussion: In light of these findings, it is imperative to conduct regular inspections and maintain the tensile state of the invert structure, which warrants the most attention. To safeguard the safety and longevity of subway tunnel invert structures, it is essential to concentrate on the aspects related to tensile stress and closely monitor train loads, specifically axle weight and speed.
Conventional and new proposals of GnRH therapy for ovarian, breast, and prostatic cancers
Authors: Garrido Maritza P. , Hernandez Andrea , Vega Margarita , Araya Eyleen , Romero Carmen
Published: March 2023

For many years, luteinizing hormone-releasing hormone or gonadotropin-releasing hormone (GnRH) analogs have been used to treat androgen or estrogen-dependent tumors. However, emerging evidence shows that the GnRH receptor (GnRH-R) is overexpressed in several cancer cells, including ovarian, endometrial, and prostate cancer cells, suggesting that GnRH analogs could exert direct antitumoral actions in tumoral tissues that express GnRH-R. Another recent approach based on this knowledge was the use of GnRH peptides for developing specific targeted therapies, improving the delivery and accumulation of drugs in tumoral cells, and decreasing most side effects of current treatments. In this review, we discuss the conventional uses of GnRH analogs, together with the recent advances in GnRH-based drug delivery for ovarian, breast, and prostatic cancer cells.
Association of antihypertensive drugs with fracture and bone mineral density: A comprehensive drug-target Mendelian randomization study
Authors: Huang Xin , Zhang Tianxin , Guo Ping , Gong Weiming , Zhu Hengchao , Zhao Meng , Yuan Zhongshang
Published: March 2023

Background

Observational studies have investigated the associations between antihypertensive drugs and fracture risk as well as bone mineral density (BMD), but yielding controversial results.

Methods

In this study, a comprehensive drug-target Mendelian randomization (MR) analysis was conducted to systematically examine the associations between genetic proxies for eight common antihypertensive drugs and three bone health-related traits (fracture, total body BMD [TB-BMD], and estimated heel BMD [eBMD]). The main analysis used the inverse-variance weighted (IVW) method to estimate the causal effect. Multiple MR methods were also employed to test the robustness of the results.

Results

The genetic proxies for angiotensin receptor blockers (ARBs) were associated with a reduced risk of fracture (odds ratio [OR] = 0.67, 95% confidence interval [CI]: 0.54 to 0.84; P = 4.42 × 10-4; P-adjusted = 0.004), higher TB-BMD (β = 0.36, 95% CI: 0.11 to 0.61; P = 0.005; P-adjusted = 0.022), and higher eBMD (β = 0.30, 95% CI: 0.21 to 0.38; P = 3.59 × 10-12; P-adjusted = 6.55 × 10-11). Meanwhile, genetic proxies for calcium channel blockers (CCBs) were associated with an increased risk of fracture (OR = 1.07, 95% CI: 1.03 to 1.12; P = 0.002; P-adjusted = 0.013). Genetic proxies for potassium sparing diuretics (PSDs) showed negative associations with TB-BMD (β = -0.61, 95% CI: -0.88 to -0.33; P = 1.55 × 10-5; P-adjusted = 1.86 × 10-4). Genetic proxies for thiazide diuretics had positive associations with eBMD (β = 0.11, 95% CI: 0.03 to 0.18; P = 0.006; P-adjusted = 0.022). No significant heterogeneity or pleiotropy was identified. The results were consistent across different MR methods.

Conclusions

These findings suggest that genetic proxies for ARBs and thiazide diuretics may have a protective effect on bone health, while genetic proxies for CCBs and PSDs may have a negative effect.
High throughput screening of fungal phytopathogens caught in Australian forestry insect surveillance traps
Authors: Trollip Conrad , Carnegie Angus J. , Piper Alexander M. , Kaur Jatinder , Martoni Francesco , Dinh Quang , Smith David , Mann Ross , Rodoni Brendan , Edwards Jacqueline
Published: March 2023

Post-border surveillance for forestry’s high priority pests and pathogens is conducted routinely through established programs focused on the main points-of-entry and across the major plantation growing regions. Currently, most diagnostic protocols used to identify fungal phytopathogens sampled during these surveys rely on traditional methods, such as morphological examination and DNA barcoding techniques. This stepwise process from isolation to species identification is often regarded as slow, expensive, and limited due to the need for disease manifestation and/or comprehensive expertise for rapid and accurate detection. In this study, we applied a recently validated high-throughput, dual-marker amplicon sequencing approach on insect surveillance traps from across Australia to assess its performance for the targeted surveillance of the Ophiostomatales, an order of fungi comprising notable phytopathogens which are vectored by bark beetles. By using a recently validated assay we were able to confidently characterize a range of Ophiostomatalean taxa known to be present in Australia, while reporting eight first detections from environmental DNA. Our study demonstrates the value of targeted multi-barcode amplicon sequencing for high-throughput screening of fungi caught in post-border surveillance traps, in addition to emphasizing research priorities that require further investigation before such methods can be implemented routinely for biosecurity.
Circulating adipokine concentrations and the risk of venous thromboembolism: A Mendelian randomization and mediation analysis
Authors: Xiao Weizhong , Li Jian , Feng Tianyuyi , Jin Long
Published: March 2023

Background: Previous observational studies have suggested that circulating adipokine concentrations are related to a greater risk of venous thromboembolism (VTE). However, it remained unclear whether these observations reflect causality.

Objective: This study aimed to investigate the causal relationship between circulating adipokine concentrations (including adiponectin, leptin, PAI-1, MCP-1, leptin receptor, and RETN) and the risk of VTE and its subtypes (DVT and PE) and to determine whether circulating adipokine concentrations are a mediator of venous thromboembolic events in obese patients.

Methods: We used Mendelian randomization (MR) analyses to determine the effects of the body mass index (BMI), adiponectin, leptin, PAI-1, MCP-1, leptin receptor, and RETN levels on VTE, DVT, and PE in a cohort of 11,288 VTE cases, 5,632 DVT cases, 5,130 PE cases, and 254,771 controls. We then assessed the proportion of the effect of obesity on VTE, DVT, and PE explained by circulating leptin levels.

Result: Genetically predicted higher BMI was related to increased VTE (OR = 1.45, p < 0.001), DVT (OR = 1.63, p < 0.001), and PE (OR = 1.37, p < 0.001) risk, and higher circulating leptin levels increase odds of VTE (OR = 1.96, q < 0.001), DVT (OR = 2.52, q < 0.001), and PE (OR = 2.26, q = 0.005). In addition, we found that the causal effect between elevated serum adiponectin and the decreased risk of VTE (OR = 0.85, p = 0.013, q = 0.053) and PE (OR = 0.81, p = 0.032, q = 0.083) and between MCP-1 and the reduced risk of VTE (OR = 0.88, p = 0.048, q = 0.143) is no longer significant after FDR adjustment. In MR mediation analysis, the mediation effect of circulating leptin levels in the causal pathway from BMI to PE was estimated to be 1.28 (0.95–1.71, p = 0.10), accounting for 39.14% of the total effect.

Conclusion: The circulating leptin level is a risk factor for VTE, DVT, and PE, but it might be a potential mediator of BMI on the risk of PE, and thus, interventions on the circulating leptin level in obesity might reduce the risk of PE. Adiponectin is a potential protective factor for both VTE and PE.
Genotype-phenotype pattern analysis of pathogenic PAX9 variants in Chinese Han families with non-syndromic oligodontia
Authors: Ren Jiabao , Gan Sifang , Zheng Shushen , Li Meikang , An Yilin , Yuan Shuo , Gu Xiuge , Zhang Li , Hou Yan , Du Qingqing , Zhang Guozhong , Shen Wenjing
Published: March 2023

Background: Non-syndromic oligodontia is characterized by the absence of six or more permanent teeth, excluding third molars, and can have aesthetic, masticatory, and psychological consequences. Previous studies have shown that PAX9 is associated with autosomal dominant forms of oligodontia but the precise molecular mechanisms are still unknown.

Methods: Whole-exome and Sanger sequencing were performed on a cohort of approximately 28 probands with NSO, for mutation analysis. Bioinformatic analysis was performed on the potential variants. Immunofluorescence assay, western blotting, and qPCR were used to explore the preliminary functional impact of the variant PAX9 proteins. We reviewed PAX9-related NSO articles in PubMed to analyze the genotype-phenotype correlations.

Results: We identified three novel PAX9 variants in Chinese Han families: c.152G>T (p.Gly51Val), c.239delC (p.Thr82Profs*3), and c.409C>T (q.Gln137Ter). In addition, two previously reported missense variants were identified: c.140G>C (p.Arg47Pro) and c.146C>T (p.Ser49Leu) (reference sequence NM_006194.4). Structural modeling revealed that all missense variants were located in the highly conserved paired domain. The other variants led to premature termination of the protein, causing structural impairment of the PAX9 protein. Immunofluorescence assay showed abnormal subcellular localizations of the missense variants (R47P, S49L, and G51V). In human dental pulp stem cells, western blotting and qPCR showed decreased expression of PAX9 variants (c.140G>C, p.R47P, and c.152G>T, p.G51V) compared with the wild-type group at both the transcription and translation levels. A review of published papers identified 64 PAX9 variants related to NSO and found that the most dominant feature was the high incidence of missing upper second molars, first molars, second premolars, and lower second molars.

Conclusion: Three novel PAX9 variants were identified in Chinese Han families with NSO. These results extend the variant spectrum of PAX9 and provide a foundation for genetic diagnosis and counseling.
Impact of BCG vaccination on the repertoire of human γδ T cell receptors
Authors: Xia Mei , Blazevic Azra , Fiore-Gartland Andrew , Hoft Daniel F.
Published: March 2023

Introduction

Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) infection is a serious threat to human health. Vaccination with BCG prevents the development of the most severe forms of TB disease in infants and was recently shown to prevent Mtb infection in previously uninfected adolescents. γδ T cells play a major role in host defense at mucosal sites and are known to respond robustly to mycobacterial infection. However, our understanding of the effects of BCG vaccination on γδ T cell responses is incomplete.

Methods

In this study we performed γδ T cell receptor (TCR) repertoire sequencing of samples provided pre- and post-BCG vaccination from 10 individuals to identify specific receptors and TCR clones that are induced by BCG.

Results

Overall, there was no change in the diversity of γTCR or δTCR clonotypes in post- vs pre-BCG samples. Furthermore, the frequencies of TCR variable and joining region genes were minimally modulated by BCG vaccination at either the γTCR or δTCR loci. However, the γTCR and δTCR repertoires of individuals were highly dynamic; a median of ~1% of γTCR and ~6% of δTCR in the repertoire were found to significantly expand or contract in post- vs pre-BCG comparisons (FDR-q < 0.05). While many of the clonotypes whose frequency changed after BCG vaccination were not shared among multiple individuals in the cohort, several shared (i.e., “public”) clonotypes were identified with a consistent increase or decrease in frequency across more than one individual; the degree of sharing of these clonotypes was significantly greater than the minimal sharing that would be expected among γTCR and δTCR repertoires. An in vitro analysis of Mtb antigen-reactive γδ T cells identified clonotypes that were similar or identical to the single-chain γTCRs and δTCRs that changed consistently after BCG vaccination; pairings of γTCRs and δTCRs that increased after BCG vaccination were significantly over-represented among the Mtb-reactive γδ T cells (p = 1.2e-6).

Discussion

These findings generate hypotheses about specific γδTCR clonotypes that may expand in response to BCG vaccination and may recognize Mtb antigens. Future studies are required to validate and characterize these clonotypes, with an aim to better understand the role of γδ T cells in Mtb immunity.
Induction of antigen specific intrahepatic CD8+ T cell responses by a secreted heat shock protein based gp96-Ig-PfCA malaria vaccine
Authors: Padula Laura , Fisher Eva , Wijayalath Wathsala , Patterson Noelle B. , Huang Jun , Ganeshan Harini , Robinson Tanisha , Bates François A. , Hanson Margaret A. , Martin Monica L. , Rivas Katelyn , Garcia Denisse , Edgel Kimberly A. , Sedegah Martha , Villasante Eileen , Strbo Natasa
Published: March 2023

Introduction

A highly efficacious and durable vaccine against malaria is an essential tool for global malaria eradication. One of the promising strategies to develop such a vaccine is to induce robust CD8+ T cell mediated immunity against malaria liver-stage parasites.

Methods

Here we describe a novel malaria vaccine platform based on a secreted form of the heat shock protein, gp96-immunoglobulin, (gp96-Ig) to induce malaria antigen specific, memory CD8+ T cells. Gp96-Ig acts as an adjuvant to activate antigen presenting cells (APCs) and chaperone peptides/antigens to APCs for cross presentation to CD8+ T cells.

Results

Our study shows that vaccination of mice and rhesus monkeys with HEK-293 cells transfected with gp96-Ig and two well-known Plasmodium falciparum CSP and AMA1 (PfCA) vaccine candidate antigens, induces liver-infiltrating, antigen specific, memory CD8+ T cell responses. The majority of the intrahepatic CSP and AMA1 specific CD8+ T cells expressed CD69 and CXCR3, the hallmark of tissue resident memory T cells (Trm). Also, we found intrahepatic, antigen-specific memory CD8+ T cells secreting IL-2, which is relevant for maintenance of effective memory responses in the liver.

Discussion

Our novel gp96-Ig malaria vaccine strategy represents a unique approach to induce liver-homing, antigen-specific CD8+ T cells critical for Plasmodium liver-stage protection.
Persistent immune activation and altered gut integrity over time in a longitudinal study of Ugandan youth with perinatally acquired HIV
Authors: Dirajlal-Fargo Sahera , Strah Monika , Ailstock Kate , Sattar Abdus , Karungi Christine , Nazzinda Rashidah , Kityo Cissy , Musiime Victor , Funderburg Nicholas , McComsey Grace A.
Published: March 2023

Introduction

Perinatally acquired HIV infection (PHIV) occurs during a critical window of immune development. We investigated changes in systemic inflammation and immune activation in adolescents with PHIV and those without HIV (HIV-) in Uganda.

Methods

A prospective observational cohort study was performed in 2017-2021 in Uganda. All participants were between 10-18 years of age and without active co-infections. PHIVs were on ART with HIV-1 RNA level ≤400 copies/mL. We measured plasma and cellular markers of monocyte activation, T-cell activation (expression of CD38 and HLA-DR on CD4+ and CD8+), oxidized LDL, markers of gut integrity and fungal translocation. Groups were compared using Wilcoxon rank sum tests. Changes from baseline were examined with 97.5% confidence intervals on relative fold change. P values were adjusted for false discovery rate.

Results

We enrolled 101 PHIV and 96 HIV-; among these, 89 PHIV and 79 HIV- also had measurements at 96 weeks. At baseline, median (Q1, Q3) age was 13 yrs (11,15), and 52% were females. In PHIV, median CD4+ cell counts were 988 cells/µL (638, 1308), ART duration was 10 yrs (8, 11), and 85% had viral load <50 copies/mL throughout the study, 53% of participants had a regimen switch between visits, 85% of whom switched to 3TC, TDF and DTG. Over 96 weeks, while hsCRP decreased by 40% in PHIV (p=0.12), I-FABP and BDG both increased by 19 and 38% respectively (p=0.08 and ≤0.01) and did not change in HIV- (p≥0.33). At baseline, PHIVs had higher monocyte activation (sCD14) (p=0.01) and elevated frequencies of non-classical monocytes (p<0.01) compared to HIV- which remained stable over time in PHIV but increased by 34% and 80% respectively in HIV-. At both time points, PHIVs had higher T cell activation (p ≤ 0.03: CD4+/CD8+ T cells expressing HLA-DR and CD38). Only in PHIV, at both timepoints, oxidized LDL was inversely associated with activated T cells(p<0.01). Switching to dolutegravir at week 96 was significantly associated an elevated level of sCD163 (β=0.4, 95% CI=0.14,0.57, p<0.01), without changes in other markers.

Conclusion

Ugandan PHIV with viral suppression have some improvement in markers of inflammation over time, however T-cell activation remains elevated. Gut integrity and translocation worsened only in PHIV over time. A deeper understanding of the mechanisms causing immune activation in ART treated African PHIV is crucial.
Circulating miRNA-19b as a biomarker of disease progression and treatment response to baricitinib in rheumatoid arthritis patients through miRNA profiling of monocytes
Authors: Ciechomska Marzena , Roszkowski Leszek , Burakowski Tomasz , Massalska Magdalena , Felis-Giemza Anna , Roura Adria-Jaume
Published: March 2023

Introduction

A number of studies have demonstrated a key role of miRNA isolated from cells, tissue or body fluids as disease-specific biomarkers of autoimmune rheumatic diseases including rheumatoid arthritis (RA) and systemic sclerosis (SSc). Also, the expression level of miRNA is changing during disease development, therefore miRNA can be used as biomarkers monitoring RA progression and treatment response. In this study we have investigated the monocytes-specific miRNA that could serve as potential biomarkers of disease progression observed in sera and synovial fluids (SF) in early (eRA) and advanced (aRA) RA and in RA patients before and 3 months after selective JAK inhibitor (JAKi) -baricitinib treatment.

Methods

Samples from healthy control (HC) (n=37), RA (n=44) and SSc (n=10) patients were used. MiRNA-seq of HC, RA, and SSc monocytes was performed to find versatile miRNA present in different rheumatic diseases. Selected miRNAs were validated in body fluids in eRA (<2 years disease onset) and aRA (>2 years disease onset) and RA patients receiving baricitinib.

Results

Using miRNA-seq, we selected top 6 miRNA out of 95 that were significantly changed in both RA and SSc monocytes compared to HC. To identify circulating miRNA predicting RA progression, these 6 miRNA were measured in eRA and aRA sera and SF. Interestingly, miRNA (-19b-3p, -374a-5p, -3614-5p) were significantly increased in eRA sera vs HC and even further upregulated in SF vs aRA sera. In contrast, miRNA-29c-5p was significantly reduced in eRA sera vs HC and even further decreased in SF vs aRA sera. Kegg pathway analysis predicted that miRNA were involved in inflammatory-mediated pathways. ROC analysis demonstrated that miRNA-19b-3p (AUC=0.85, p=0.04) can be used as biomarker predicting JAKi response.

Discussion

In conclusion, we identified and validated miRNA candidates which were present simultaneously in monocytes, sera, SF and that can be used as biomarkers predicting joint inflammation and monitoring therapy response to JAKi in RA patients.
Factors influencing dietary patterns among the youth from higher educational institutions in India
Authors: Soam Sudhir K. , Yashavanth B. S. , Dhumantarao Thammi Raju , Maruthamuthu Balakrishnan , Balasani Raghupathi , Rakesh S.
Published: March 2023

Purpose

To determine the factors influencing the dietary habits of the varied groups among adults in India.

Design/approach

Data on food habits such as choice of diet, preference toward meat, spicy food, sugar/calorie etc., were collected from the participants (from several higher education institutions) of different training programmes and events organised at ICAR-NAARM, Hyderabad and its students of Post Graduate Diploma in Agribusiness Management.

Findings

Results of the study indicated that the food choice of the respondents is highly influenced by their region, age and gender. Most of the respondents preferred vegetarian food with increasing age. We also noticed that as age of the respondents increased, their preference toward simple & plain food (with less oil/spice) also increased. From the present investigation, it is recommended that the customized food menu should be prepared in every food serving institution based on the region, age and gender of the consumer.

Novelty

Analysis of dietary patterns can be helpful for doctors, dieticians, food policy-making, restaurateurs, youth hostels, food organisations, mega kitchens etc. that would also contribute to responsible food consumption.
Efficacy and tolerability of perampanel in patients with seizures in real-world clinical practice: A systematic review and meta-analysis
Authors: Hou Liyan , Yang Jingjing , Zhang Xuan , Li Na , Li Sheng , Zhang Lei , Zhao Jie , Wang Qingshan
Published: March 2023

Objectives: The aim of this study was to systematically review the efficacy and tolerability of perampanel (PER) when used as add-on treatment or monotherapy in patients with epilepsy aged 12 years and older in routine clinical practice.

Methods: Electronic and clinical trials databases were searched for observational studies of PER published up to 1 March 2022. The outcomes of interest were responder rates, adverse effects (AEs), and withdrawal rates. Subgroup analyses were performed to explore the potential factors that might affect the efficacy and safety of PER usage.

Results: A total of 56 studies, which included 10,688 patients, were enrolled. The results showed that after 3, 6, and 12 months of PER treatment, the pooled 50% responder rates in patients with epilepsy were 50.0% (95% CI: 0.41–0.60), 44.0% (95% CI: 0.38–0.50), and 39.0% (95% CI: 0.31–0.48), respectively, and the pooled seizure-free rates were 24.0% (95% CI: 0.17–0.32), 21.0% (95% CI: 0.17–0.25), and 20.0% (95% CI: 0.16–0.24), respectively. Subgroup analyses revealed that the efficacy of PER could be affected by the way in which PER is administrated. Patients in the groups where PER was used as the first add-on, primary monotherapy, or combined with non–enzyme-inducing AEDs (non-EIAEDs) displayed a high 50% responder rate and seizure-free rate when compared with those in the late add-on, conversion therapy, or combined with the EIAEDs groups, respectively. Furthermore, the incidences of AEs at 3, 6, and 12 months of PER treatment were 46% (95% CI: 0.38–0.55), 52.0% (95% CI: 0.43–0.60), and 46.0% (95% CI: 0.40–0.52), respectively. The withdrawal rates due to AEs were 8.0% (95% CI: 0.06–0.11), 16.0% (95% CI: 0.13–0.20), and 16% (95% CI: 0.11–0.21) at 3, 6, and 12 months of PER treatment, respectively. Subgroup analyses showed a higher withdrawal rate in the rapid (30%, 95% CI: 0.22–0.38) than in the slow (12%, 95% CI: 0.06–0.18) titration group.

Conclusion: Altogether, PER was effective and could be fairly tolerated in both short-term and long-term usage in patients with epilepsy in routine clinical practice. Furthermore, PER appeared to be more effective when PER was used as the first add-on, monotherapy, or concomitant with non-EIAEDs.

Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022384532.
Effects of different concentrations of biochar amendments and Pb toxicity on rhizosphere soil characteristics and bacterial community of red clover (Trifolium pretense L.)
Authors: Meng Lingdong , Wu Yuchen , Mu Meiqi , Wang Zicheng , Chen Zirui , Wang Lina , Ma Zewang , Cui Guowen , Yin Xiujie
Published: March 2023

Amending soil with biochar can reduce the toxic effects of heavy metals (HM) on plants and the soil. However, the effects of different concentrations of biochar on the properties and microbial activities in lead (Pb)-contaminated soils are unclear. In this study, two Pb concentrations were set (low, 1000 mg/kg; high, 5000 mg/kg), and five corn straw biochar (CSB) concentrations (0, 2.5, 5, 10 and 15%) were used to determine the response of the growth and rhizosphere of red clover (Trifolium pretense L.) (in terms of soil properties and bacteria) to CSB and Pb application. The results showed that 5% CSB better alleviated the toxicity of Pb on the shoot length of red clover, the biomass increased by 74.55 and 197.76% respectively and reduced the enrichment factor (BCF) and transport factor (TF) of red clover. Pb toxicity reduced soil nutrients, catalase (CAT), acid phosphatase (ACP) and urease activity, while the addition of CSB increased soil pH, soil organic matter (SOM) content and soil enzyme activity. 16S rDNA amplicon sequencing analysis showed that Pb toxicity reduced the diversity of rhizosphere bacteria in red clover and reduced the relative abundance of plant growth-promoting rhizobacteria such as Gemmatimonas, Devosia and Bryobacter. Spearman correlation analysis showed that the addition of alkaline CSB restored the relative abundance of rhizobacteria positively correlated with pH, such as Chitinophaga, Sphingomonas, Devosia and Pseudomonas, and thus restored the rhizosphere soil environment. This study demonstrates that 5% CSB can better alleviate the toxicity of Pb to red clover and soil. We also provide a theoretical basis for the subsequent use of beneficial bacteria to regulate the repair efficiency of red clover.
All-in-one aerial image enhancement network for forest scenes
Authors: Chen Zhaoqi , Wang Chuansheng , Zhang Fuquan , Zhang Ling , Grau Antoni , Guerra Edmundo
Published: March 2023

Drone monitoring plays an irreplaceable and significant role in forest firefighting due to its characteristics of wide-range observation and real-time messaging. However, aerial images are often susceptible to different degradation problems before performing high-level visual tasks including but not limited to smoke detection, fire classification, and regional localization. Recently, the majority of image enhancement methods are centered around particular types of degradation, necessitating the memory unit to accommodate different models for distinct scenarios in practical applications. Furthermore, such a paradigm requires wasted computational and storage resources to determine the type of degradation, making it difficult to meet the real-time and lightweight requirements of real-world scenarios. In this paper, we propose an All-in-one Image Enhancement Network (AIENet) that can restore various degraded images in one network. Specifically, we design a new multi-scale receptive field image enhancement block, which can better reconstruct high-resolution details of target regions of different sizes. In particular, this plug-and-play module enables it to be embedded in any learning-based model. And it has better flexibility and generalization in practical applications. This paper takes three challenging image enhancement tasks encountered in drone monitoring as examples, whereby we conduct task-specific and all-in-one image enhancement experiments on a synthetic forest dataset. The results show that the proposed AIENet outperforms the state-of-the-art image enhancement algorithms quantitatively and qualitatively. Furthermore, extra experiments on high-level vision detection also show the promising performance of our method compared with some recent baselines.
Setting limits to tolerance: An experimental investigation of individual reactions to extremism and violence
Authors: Trüdinger Eva-Maria , Ziller Conrad
Published: March 2023

Tolerating others' opinions, even if disliked, is a cornerstone of liberal democracy. At the same time, there are limits to political tolerance as tolerating extremists and groups who use violence would threaten the foundations of tolerance itself. We study people's willingness to set limits to tolerance in case of violence and extremism (scope of tolerance)—under different conditions regarding ideological groups (left-wing, right-wing, religious) and offline/online contexts of free speech. Using data from a large-scale survey experiment conducted in Germany, we show that citizens clearly set limits to tolerance of different groups, especially if the latter have violent intentions, and that people tend to be more tolerant online than offline. Moreover, we find that citizens are more tolerant toward groups that are closer to their own ideological stance. However, violence disrupts such an ideological bias as respondents across the ideological spectrum exhibit low levels of tolerance toward violent groups—irrespectively of their political stance. Our findings highlight the importance of situational factors as foundations of judgments on the limits to tolerance.
Coaching leadership and creative performance: A serial mediation model of psychological empowerment and constructive voice behavior
Authors: Hwang Chan Young , Kang Seung-Wan , Choi Suk Bong
Published: March 2023

This study empirically analyzes the role of coaching leadership in enhancing an organization’s creative performance, discussing and evaluating important mediating paths of coaching leadership regarding creative performance. As a result of an empirical analysis based on survey data collected from 332 employees of Korean companies, this study first confirms that coaching leadership has a positive effect on both employees’ creative performance. We also found that psychological empowerment and constructive voice behavior positively mediated the relationship between coaching leadership and creative performance. Finally, the serial mediating effect of coaching leadership on creative performance was tested through psychological empowerment and constructive voice behavior and confirmed to have a positive effect. This study indicates the importance of leadership as a critical variable that promotes employees’ creative performance. In addition, by confirming the serial mediating role of psychological empowerment and constructive voice behavior, this study improves understanding of key mechanism in which coaching leadership leads to creative performance.
Individual differences moderate effects in an Unusual Disease paradigm: A psychophysical data collection lab approach and an online experiment
Authors: Wyszynski Marc , Diederich Adele
Published: March 2023

We report two studies investigating individual intuitive-deliberative cognitive-styles and risk-styles as moderators of the framing effect in Tversky and Kahneman's famous Unusual Disease problem setting. We examined framing effects in two ways: counting the number of frame-inconsistent choices and comparing the proportions of risky choices depending on gain-loss framing. Moreover, in addition to gain-loss frames, we systematically varied the number of affected people, probabilities of surviving/dying, type of disease, and response deadlines. Study 1 used a psychophysical data collection approach and a sample of 43 undergraduate students, each performing 480 trials. Study 2 was an online study incorporating psychophysical elements in a social science approach using a larger and more heterogeneous sample, i.e., 262 participants performed 80 trials each. In both studies, the effect of framing on risky choice proportions was moderated by risk-styles. Cognitive-styles measured on different scales moderated the framing effect only in study 2. The effects of disease type, probability of surviving/dying, and number of affected people on risky choice frequencies were also affected by cognitive-styles and risk-styles but different for both studies and to different extents. We found no relationship between the number of frame-inconsistent choices and cognitive-styles or risk-styles, respectively.
Improving memory for unusual events with wakeful reactivation
Authors: Glicksohn Arit , Shams Ladan , Seitz Aaron R.
Published: March 2023

Memory consists of multiple processes, from encoding information, consolidating it into short- and long- term memory, and later retrieving relevant information. Targeted memory reactivation is an experimental method during which sensory components of a multisensory representation (such as sounds or odors) are ‘reactivated’, facilitating the later retrieval of unisensory attributes. We examined whether novel and unpredicted events benefit from reactivation to a greater degree than normal stimuli. We presented participants with everyday objects, and ‘tagged’ these objects with sounds (e.g., animals and their matching sounds) at different screen locations. ‘Oddballs’ were created by presenting unusual objects and sounds (e.g., a unicorn with a heartbeat sound). During a short reactivation phase, participants listened to a replay of normal and oddball sounds. Participants were then tested on their memory for visual and spatial information in the absence of sounds. Participants were better at remembering the oddball objects compared to normal ones. Importantly, participants were also better at recalling the locations of oddball objects whose sounds were reactivated, compared to objects whose sounds that were not presented again. These results suggest that episodic memory benefits from associating objects with unusual cues, and that reactivating those cues strengthen the entire multisensory representation, resulting in enhanced memory for unisensory attributes.
Risk of recurrent cardiovascular events in coronary artery disease patients with Type D personality
Authors: Torgersen Kristin Stensland , Sverre Elise Christine Bjørkholen , Weedon-Fekjær Harald , Andreassen Ole A. , Munkhaugen John , Dammen Toril
Published: March 2023

Introduction

Data on the association between Type D personality, its traits negative affectivity (NA) and social inhibition (SI), and risk of major adverse cardiac events (MACE) in coronary outpatients is sparse. Furthermore, the associations between Type D subgroups and cardiovascular risk factors are largely unknown.

Methods

We investigated i) Type D personality, NA and SI and risk of recurrent MACE, and ii) the relationship between Type D subgroups and risk factors in a coronary population. This prospective cohort study included 1083 patients` median 16 months after a myocardial infarction and/or a revascularization procedure who were followed-up for 4.2 (SD 0.4) years. Type D personality was assessed by DS14. Anxiety and depression, statin adherence, and risk factors were assessed by patients’ self-report and a clinical examination with blood samples. MACE, defined as cardiovascular death, myocardial infarction, revascularization, stroke or heart failure, were obtained from hospital records from index event to end of study lasting 5.7 years. Data were analyzed by Cox proportional hazard regression.

Results

In all, 352 MACE occurred in 230 patients after average 4.2 years follow-up. Higher NA score was associated with MACE after adjustment for age, risk factors and comorbidity (HR 1.02 per unit increase, 95% CI 1.00-1.05), whereas we found a weaker, not statistically significant estimated effect of higher SI score. After additional adjustment for symptoms of anxiety and depression, we found a weaker, not statistically significant association between NA and MACE (HR 1.01 per unit increase, 95% CI 0.98-1.05). Low statin adherence and smoking were more prevalent in the Type D and high NA group.

Discussion

Our results indicate that the NA trait is related to worse prognosis in outpatients with coronary artery disease.
Sustainable entrepreneurship out of entrepreneurial opportunity identification: The mediating role of psychological capital
Authors: Zhang Hongxin , Chen Hongxia
Published: March 2023

Introduction

The aim of the present study, was to examine the simultaneous effects of entrepreneurial opportunity identification (EOI) and psychological capital (PC) on university students' entrepreneurial intention (EI). Compared with necessity-driven entrepreneurship, opportunity-driven entrepreneurship is more sustainable. Scholars have shown that EOI is key to forming EI, but little has been discussed about its association with PC.

Methods

A total of 555 university students in China were enrolled by means of convenience sampling. Descriptive statistics and correlation analysis of variables were performed using SPSS 21.0 software. Structural equation modeling analysis (SEM) with AMOS 21.0 was used to examine the structural effects of EOI and PC on university students' EI.

Results

According to the results, university students' EOI and PC had a positive and insignificant influence on their levels of EI. Furthermore, PC was found to fully mediate the impact of EOI on EI.

Discussion

The present study could shed light on new instructions to examine the interaction between the cognitive and psychological components of EI in the field of entrepreneurship. It is recommended that educators and practitioners should pay regard to the role of EOI and PC.
Preemptive endoluminal vacuum therapy with the VACStent—A pilot study to reduce anastomotic leakage after Ivor Lewis hybrid esophagectomy
Authors: Lange Jonas , Eisenberger Claus Ferdinand , Knievel Judith , Linderer Anne , Heiss Markus Maria
Published: March 2023

Introduction

Endoscopic treatment by vacuum therapy (EVT) or covered stents has emerged as an improved treatment option for upper gastrointestinal wall defects and is regarded as an improved treatment option for anastomotic leakage (AL) after esophagectomy. However, endoluminal EVT devices may lead to obstruction of the GI tract; and a high rate of migration and missing functional drainage has been shown for covered stents. The recently developed VACStent, a combination of a fully covered stent within a polyurethane sponge cylinder may overcome these issues allowing EVT while stent passage is still open. Initial clinical applications have demonstrated efficacy, practicability and safety in the treatment of esophageal leaks (AL).

Methods

In this pilot study, 9 patients with high-risk anastomosis after neoadjuvant therapy undergoing hybrid esophagectomy received the VACStent in a preemptive setting for the assessment of the reduction of the AL rate, postoperative morbidity and mortality.

Results

Technical success of the application of the VACStent® was achieved in all interventions. One patient experienced anastomotic leakage 10 days after esophagectomy and was successfully treated with two consecutive VACStents and a VAC Sponge. In summary, mortality in-hospital was 0% and anastomotic healing was uneventful without septic episodes. No severe device-related adverse events (SADE) nor significant local bleeding or erosion could be observed. Oral intake of liquids or food was documented in all patients. The device handling was regarded uncomplicated.

Discussion

The preemptive application of the VACStent offers a promising new option for improved clinical treatment avoiding of critical situations in hybrid esophagectomy, which should be validated in a large clinical study.
Impact of resveratrol-loaded liposomal nanocarriers on heat-stressed broiler chickens: Effects on performance, sirtuin expression, oxidative stress regulators, and muscle building factors
Authors: Kishawy Asmaa T. Y. , Ibrahim Doaa , Roushdy Elshimaa M. , Moustafa Amira , Eldemery Fatma , Hussein Elham M. , Hassan Fardos A. M. , Elazab Sara T. , Elabbasy Mohamed Tharwat , Kanwal Raheela , Kamel Walid M. , Atteya Mohamed R. , Zaglool Asmaa W.
Published: March 2023

Climate change is considered to be the primary cause of heat stress (HS) in broiler chickens. Owing to the unique properties of extracted polyphenols, resveratrol-loaded liposomal nanoparticles (Resv-Lipo NPs) were first explored to mitigate the harmful effects of HS. The dietary role of Resv-Lipo NPs in heat-stressed birds was investigated based on their growth performance, antioxidative potential, and the expression of heat shock proteins, sirtuins, antioxidant, immune, and muscle-building related genes. A total of 250 1-day-old Ross 308 broiler chickens were divided into five experimental groups (5 replicates/group, 10 birds/replicate) for 42 days as follows: the control group was fed a basal diet and reared in thermoneutral conditions, and the other four HS groups were fed a basal diet supplemented with Resv-Lipo NPsI, II, and III at the levels of 0, 50, 100, and 150 mg/kg diet, respectively. The results indicated that supplementation with Resv-Lipo NP improved the growth rate of the HS group. The Resv-Lipo NP group showed the most significant improvement in body weight gain (p < 0.05) and FCR. Additionally, post-HS exposure, the groups that received Resv-Lipo NPs showed restored functions of the kidney and the liver as well as improvements in the lipid profile. The restoration occurred especially at higher levels in the Resv-Lipo NP group compared to the HS group. The elevated corticosterone and T3 and T4 hormone levels in the HS group returned to the normal range in the Resv-Lipo NPsIII group. Additionally, the HS groups supplemented with Resv-Lipo NPs showed an improvement in serum and muscle antioxidant biomarkers. The upregulation of the muscle and intestinal antioxidant-related genes (SOD, CAT, GSH-PX, NR-f2, and HO-1) and the muscle-building genes (myostatin, MyoD, and mTOR) was observed with increasing the level of Resv-Lipo NPs. Heat stress upregulated heat shock proteins (HSP) 70 and 90 gene expression, which was restored to normal levels in HS+Resv-Lipo NPsIII. Moreover, the expression of sirtuin 1, 3, and 7 (SIRT1, SIRT3, and SIRT7) genes was increased (p < 0.05) in the liver of the HS groups that received Resv-Lipo NPs in a dose-dependent manner. Notably, the upregulation of proinflammatory cytokines in the HS group was restored in the HS groups that received Resv-Lipo NPs. Supplementation with Resv-Lipo NPs can mitigate the harmful impact of HS and consequently improve the performance of broiler chickens.