bluuebunny/crossref_metadata_2025 huggingface dataset:
A Personalized Approach to Radical Cystectomy Can Decrease Its Complication Rates
Authors: Adamczyk Przemyslaw , Poblocki Pawel , Michalik Cyprian , Kadlubowski Mateusz , Adamowicz Jan , Mikolajczak Witold , Drewa Tomasz , Juszczak Kajetan
Published: February 2022

The aim of this study was to assess the influence of a patient’s general status on perioperative morbidity and mortality after radical cystectomy, and to assess which of the used scales is best for the prediction of major complications. The data of 331 patients with muscle-invasive bladder cancer, who underwent radical cystectomy, were analyzed. The general status was assessed according to the American Society of Anesthesiologists (ASA), Charlson Comorbidity Index (CCI), Eastern Cooperative Oncology Group (ECOG), and Geriatric-8 (G-8) scales. Complications were classified according to the Clavien–Dindo classification system. In a group of patients with the highest complication rate according to the Clavien–Dindo scale, (i) statistically more patients rated high according to the ASA and ECOG scales, (ii) patients had significantly higher CCI scores (minor complications (I-II), and (iii) there were significantly more patients rated as frail with G8—predominantly those with 11 points or fewer in the scale. A patient’s general status should be assessed before the start of therapy because patients with a high risk of death or serious complications (evaluated with any rating scale) should be offered conservative treatment. None of the scales can describe the risk of cystectomy, because the percentage of patients with major complications among those who achieved worse score results on any scale was not significantly different from the percentage of patients with major complications in the general group.
Predictors of Excess Return in a Green Energy Equity Portfolio: Market Risk, Market Return, Value-at-Risk and or Expected Shortfall?
Authors: Abraham Rebecca , El-Chaarani Hani , Tao Zhi
Published: February 2022

The rapid growth of electric vehicles, solar roofs, and wind power suggests that the potential growth in green equity investments is an emerging trend. Accordingly, this study measured the predictors of excess equity returns in a portfolio of global green energy producers, from 2010 to 2019. Fixed-effects panel data regressions of daily returns, followed by quantile regressions, were performed. There was some support for the explanation of green equity returns by market returns and market risk (beta), as indicated by the single-factor Capital Asset Pricing Model (CAPM), and the multifactor Fama–French Three-Factor and Fama–French Five-Factor Models. The most significant predictors of green equity returns were Value-at-Risk at a 95% confidence level, and Value-at-Risk at a 99% confidence level. Expected Shortfall was another extreme risk value measure. The importance of extreme value measures suggests the presence of fat-tailed leptokurtic distributions, whereby excess returns were explained by the risk of loss given adverse conditions, primarily at 95% confidence. We conclude that the proliferation of small firms and new entrants in the renewable energy sector has led to the explanation of returns by extreme values of risk.
Effect of Short-Term Zero Tillage and Legume Intercrops on Soil Quality, Agronomic and Physiological Aspects of Cotton under Arid Climate
Authors: Saleem Muhammad Faisal , Ghaffar Abdul , Rahman Muhammad Habib ur , Imran Muhammad , Iqbal Rashid , Soufan Walid , Danish Subhan , Datta Rahul , Rajendran Karthika , EL Sabagh Ayman
Published: February 2022

A detailed field experiment was carried out to study the effect of conventional and zero tillage and legume intercrops on soil health indicators and cotton productivity and also yield components of leguminous crops at the Research Farm, MNS-University of Agriculture, Multan, Pakistan. The field experiment was comprised of four legume intercrops (no intercrops (sole cotton), mung bean, mash bean, and soybean) and two tillage systems (three years zero tillage (ZT) and long-term conventional tillage (CT)). The CT showed the highest plant height (121 cm), total bolls per plant (22.9 bolls), boll weight (2.74 g) and seed yield (2031 kg ha−1) of the cotton crop, as compared to ZT. The highest leaf transpiration rate (9.28 mmol H2O m−2 s−1), net leaf photosynthetic rate (27.17 µmol m−2 s−1), stomatal conductance (0.493 mmol m−2 s−1), chlorophyll content (62.3 SPAD value), plant height (123 cm), total bolls per plant (24.4), boll weight (2.83 g), and seed yield (2090 kg ha−1) of cotton crop were recorded when it was grown as a sole crop, as compared to legume intercrops. However, soil organic matter (0.77%), phosphorus (8.08 mg kg−1), potassium (253 mg kg−1), and microbial population (7.26 × 106 Cfu) were higher in ZT than in CT. Mung bean showed a maximum number of pods (32), seed yield (173 kg ha−1), biomass (950 kg ha−1), and harvest index (19.0%), when intercropped with cotton. The highest land equivalent ratio and area time equivalent ratio were recorded in mung bean and cotton intercropping, grown under a CT system. Furthermore, the maximum benefit-cost ratio was recorded in mung bean and cotton intercropping, over sole cotton cropping under CT (1.75) and ZT (1.67) systems. The ZT and intercropping of leguminous crops with cotton might be a promising option for increasing the seed cotton yield, seed yield of leguminous crops, system profitability, and sustainability of soil health.
Assessment of Contamination Management Caused by Copper and Zinc Cations Leaching and Their Impact on the Hydraulic Properties of a Sandy and a Loamy Clay Soil
Authors: Angelaki Anastasia , Dionysidis Alkiviadis , Sihag Parveen , Golia Evangelia E.
Published: February 2022

Soil hydraulic properties are crucial to agriculture and water management and depend on soil structure. The impact of Cu and Zn cations on the hydraulic properties of sandy and loamy clay soil samples of Central Greece, was investigated in the present study. Metal solutions with increased concentrations were used to contaminate the soil samples and the effect on hydraulic properties was evaluated, demonstrating the innovation of the current study. The soil samples were packed separately into transparent columns and the initial values of hydraulic conductivity, cumulative infiltration, infiltration rate and sorptivity were estimated. In order to evaluate soil adsorption, metal concentrations were measured at the water leachate. After the contamination of the soil samples, the hydraulic properties under investigation were determined again, using distilled water as the incoming fluid; the differences at the hydraulic parameters were observed. After doubling metal concentrations into the incoming solution of loamy clay soil, metal adsorption and the values of the hydraulic parameters increased significantly. Loamy clay soil showed interaction between the clay particles and the positive charge in the incoming fluid, which led to a possible increase in aggregation. Furthermore, aggregation may led to pore generation. Contamination of sandy soil exhibited no impact on aggregation and soil structure. In order to evaluate the differences on the hydraulic properties and soil structure, the experimental points were approximated with two infiltration models.
Omics and Male Infertility: Highlighting the Application of Transcriptomic Data
Authors: Omolaoye Temidayo S. , Omolaoye Victor A. , Kandasamy Richard K. , Hachim Mahmood Yaseen , Du Plessis Stefan S.
Published: February 2022

Male infertility is a multifaceted disorder affecting approximately 50% of male partners in infertile couples. Over the years, male infertility has been diagnosed mainly through semen analysis, hormone evaluations, medical records and physical examinations, which of course are fundamental, but yet inefficient, because 30% of male infertility cases remain idiopathic. This dilemmatic status of the unknown needs to be addressed with more sophisticated and result-driven technologies and/or techniques. Genetic alterations have been linked with male infertility, thereby unveiling the practicality of investigating this disorder from the “omics” perspective. Omics aims at analyzing the structure and functions of a whole constituent of a given biological function at different levels, including the molecular gene level (genomics), transcript level (transcriptomics), protein level (proteomics) and metabolites level (metabolomics). In the current study, an overview of the four branches of omics and their roles in male infertility are briefly discussed; the potential usefulness of assessing transcriptomic data to understand this pathology is also elucidated. After assessing the publicly obtainable transcriptomic data for datasets on male infertility, a total of 1385 datasets were retrieved, of which 10 datasets met the inclusion criteria and were used for further analysis. These datasets were classified into groups according to the disease or cause of male infertility. The groups include non-obstructive azoospermia (NOA), obstructive azoospermia (OA), non-obstructive and obstructive azoospermia (NOA and OA), spermatogenic dysfunction, sperm dysfunction, and Y chromosome microdeletion. Findings revealed that 8 genes (LDHC, PDHA2, TNP1, TNP2, ODF1, ODF2, SPINK2, PCDHB3) were commonly differentially expressed between all disease groups. Likewise, 56 genes were common between NOA versus NOA and OA (ADAD1, BANF2, BCL2L14, C12orf50, C20orf173, C22orf23, C6orf99, C9orf131, C9orf24, CABS1, CAPZA3, CCDC187, CCDC54, CDKN3, CEP170, CFAP206, CRISP2, CT83, CXorf65, FAM209A, FAM71F1, FAM81B, GALNTL5, GTSF1, H1FNT, HEMGN, HMGB4, KIF2B, LDHC, LOC441601, LYZL2, ODF1, ODF2, PCDHB3, PDHA2, PGK2, PIH1D2, PLCZ1, PROCA1, RIMBP3, ROPN1L, SHCBP1L, SMCP, SPATA16, SPATA19, SPINK2, TEX33, TKTL2, TMCO2, TMCO5A, TNP1, TNP2, TSPAN16, TSSK1B, TTLL2, UBQLN3). These genes, particularly the above-mentioned 8 genes, are involved in diverse biological processes such as germ cell development, spermatid development, spermatid differentiation, regulation of proteolysis, spermatogenesis and metabolic processes. Owing to the stage-specific expression of these genes, any mal-expression can ultimately lead to male infertility. Therefore, currently available data on all branches of omics relating to male fertility can be used to identify biomarkers for diagnosing male infertility, which can potentially help in unravelling some idiopathic cases.
Reliability of Frail and Barthel Tests for Detecting Frailty in Palliative Oncological Patients in a Home Hospitalization Unit: A Comparative Study
Authors: León-Ramón Susana , Navarro-Flores Emmanuel , Losa-Iglesias Marta Elena , Becerro-de-Bengoa-Vallejo Ricardo , Jiménez-Cebrián Ana María , Romero-Morales Carlos , Palomo-López Patricia , López-López Daniel
Published: February 2022

Cancer is a condition that can increase the risk of frailty. In addition, palliative oncological patients in home hospitalization can find their activities of daily living affected. The main objective was to measure the degree of frailty in the oncological population in home hospitalization comparing Barthel and Frail-VIG Indexes. This is a descriptive cross-sectional study. A sample of oncological patients in home hospitalization (n = 50) that included 27 men and 23 women were recruited, and disability due to frailty was measured using the VIG frailty index and the Barthel scale for Activities of Daily Living (ADLs). Spearman’s correlation coefficients were categorized as weak (rs ≤ 0.40), moderate (0.41 ≤ rs ≥ 0.69) or robust (0.70 ≤ rs ≥ 1.00), with a strong indirect correlation between the domains using the toilet, transferring and wandering on the Frail-VIG scale with an r (s) value −0.810 (p < 0.001), −0.831 (p < 0.001) and −0.805 (p < 0.001), respectively, and a moderate indirect correlation for the domains of eating −0.718 (p < 0.001), dressing −0.770 (p < 0.001) and urination −0.704 (<0.001). The Frail-VIG index above 0.35 points, that is, from moderate to severe, does not affect ADLs except in the nutritional dimension in a palliative oncological population in home hospitalization. The preliminary outcomes obtained should be considered to determine the impact of nutritional status with regard to ADLs in palliative oncological patients in a home hospitalization unit.
Statistical Feature Construction for Forecasting Accuracy Increase and Its Applications in Neural Network Based Analysis
Authors: Gorshenin Andrey , Kuzmin Victor
Published: February 2022

This paper presents a feature construction approach called Statistical Feature Construction (SFC) for time series prediction. Creation of new features is based on statistical characteristics of analyzed data series. First, the initial data are transformed into an array of short pseudo-stationary windows. For each window, a statistical model is created and characteristics of these models are later used as additional features for a single window or as time-dependent features for the entire time series. To demonstrate the effect of SFC, five plasma physics and six oceanographic time series were analyzed. For each window, unknown distribution parameters were estimated with the method of moving separation of finite normal mixtures. First four statistical moments of these mixtures for initial data and increments were used as additional data features. Multi-layer recurrent neural networks were trained to create short- and medium-term forecasts with a single window as input data; additional features were used to initialize the hidden state of recurrent layers. A hyperparameter grid-search was performed to compare fully-optimized neural networks for original and enriched data. A significant decrease in RMSE metric was observed with a median of 11.4%. There was no increase in RMSE metric in any of the analyzed time series. The experimental results have shown that SFC can be a valuable method for forecasting accuracy improvement.
On Indeterminacy of Interval Multiplicative Pairwise Comparison Matrix
Authors: Kuo Ting , Chen Ming-Hui
Published: February 2022

The interval multiplicative pairwise comparison matrix (IMPCM) is widely used to model human judgments affected by uncertainty and/or ambiguity. To improve the quality of an IMPCM, consistency is not sufficient. The indeterminacy should also be within an acceptable threshold because a consistent IMPCM may be deemed unacceptable due to high indeterminacy. Regarding indeterminacy, two metrics have been proposed in the literature: the indeterminacy ratio and the indeterminacy index. The former is from a local view, and the latter is from a global view. We have proposed an acceptable IMPCM model, which guarantees that an inconsistent IMPCM can be transformed into a consistent IMPCM, and the maximal indeterminacy ratio can be reduced. However, there is still a research gap. That is, a concomitant question naturally arises: can the indeterminacy index be reduced as well? In this paper, we further prove that the indeterminacy index of an originally inconsistent IMPCM can be reduced under the proposed model. Three numerical examples are presented to illustrate the feasibility and superiority of the proposed model. We also flowcharted the proposed model from a pragmatic view such that we can judiciously reduce the indeterminacy index of the IMPCM to a certain satisfactory level. That is, by applying the proposed model once, the original inconsistent IMPCM can be transformed into a consistent IMPCM that will possess less indeterminacy than the original one has. Consequently, by successively applying the proposed model, we can reduce or even eventually eliminate the indeterminacy of the IMPCM. In other words, we can/may obtain an MPCM rather than an IMPCM. In addition to mathematical proofs, we present experimental results of computer simulations to corroborate our argument. In summary, this model is not only effective but also efficient because it only requires arithmetic operations without solving complex optimization problems.
Dual Sensory Impairment: The Correlation between Age Related Macular Degeneration and Sensorineural Hearing Loss
Authors: Istrate Marina , Hasbei-Popa Mihai , Iliescu Daniela Adriana , Ghiță Ana Cristina , Vlaicu Brigitha , Ghiță Mihai Aurelian
Published: February 2022

The pathogeneses of age-related macular degeneration (AMD) and age-related hearing impairment are not yet fully understood. If AMD and age-related hearing impairment are correlated, the cause of both may be a result of a common vulnerability. The aim of this study was to assess the interrelation between age-related macular degeneration and age-related hearing loss. Material and methods: In our case-control analysis, the hearing conditions of 40 subjects with AMD were compared with 40 age-matched healthy controls. In all patients, retinal changes were certified by clinical examinations, optical coherence tomography (OCT), and fluorescein angiography (FA). All subjects were inspected with pure tone audiometry (PTA), impedance audiometry, and speech audiometry. Results: A significant correlation (p < 0.001) was identified between age-related macular degeneration and age-related hearing impairment. The predominant hearing impairment in this case was sensorineural (SNHL). Of the patients diagnosed with AMD, SNHL was found in 88.89% of those with exudative macular degeneration and in 67.74% of those with atrophic macular degeneration. In contrast, we found that a significant proportion of the control group had normal hearing. Conclusion: One possible explanation for the association between retinal and cochlear impairment may be due to a melanin disorder.
The Effect of Free Volume on the Crystallization of Al87Ni8Gd5 Amorphous Alloy
Authors: Abrosimova Galina , Chirkova Valentina , Pershina Elena , Volkov Nikita , Sholin Ilia , Aronin Aleksandr
Published: February 2022

The effect of free volume on the process of crystallization of an Al87Ni8Gd5 amorphous alloy is investigated. The deformation of the amorphous alloys leads to the formation of shear bands, which contain an enhanced free volume concentration. To retain the free volume the amorphous alloy was coated with a layer of a refractory metal. The structure of the Al87Ni8Gd5 alloy with a protective Ta coating was studied by X-ray diffraction and transmission electron microscopy methods. The fraction of the nanocrystalline phase formed in the amorphous samples with a protective Ta coating under annealing was found to be larger than that in the uncoated samples. The size of Al nanocrystals formed in the coated and uncoated samples is the same. A higher rate of crystal nucleation in the deformed amorphous samples with a protective coating is caused by a higher diffusion coefficient due to an enhanced free volume concentration.
Calculation of Tc of Superconducting Elements with the Roeser–Huber Formalism
Authors: Koblischka Michael Rudolf , Koblischka-Veneva Anjela
Published: February 2022

The superconducting transition temperature, Tc, can be calculated for practically all superconducting elements using the Roeser–Huber formalism. Superconductivity is treated as a resonance effect between the charge carrier wave, i.e., the Cooper pairs, and a characteristic distance, x, in the crystal structure. To calculate Tc for element superconductors, only x and information on the electronic configuration is required. Here, we lay out the principles to find the characteristic lengths, which may require us to sum up the results stemming from several possible paths in the case of more complicated crystal structures. In this way, we establish a non-trivial relation between superconductivity and the respective crystal structure. The model enables a detailed study of polymorphic elements showing superconductivity in different types of crystal structures like Hg or La, or the calculation of Tc under applied pressure. Using the Roeser–Huber approach, the structure-dependent different Tc’s of practically all superconducting elements can nicely be reproduced, demonstrating the usefulness of this approach offering an easy and relatively simple calculation procedure, which can be straightforwardly incorporated in machine-learning approaches.
MALDI-TOF MS Using a Custom-Made Database, Biomarker Assignment, or Mathematical Classifiers Does Not Differentiate Shigella spp. and Escherichia coli
Authors: van den Beld Maaike J. C. , Rossen John W. A. , Evers Noah , Kooistra-Smid Mirjam A. M. D. , Reubsaet Frans A. G.
Published: February 2022

Shigella spp. and E. coli are closely related and cannot be distinguished using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) with commercially available databases. Here, three alternative approaches using MALDI-TOF MS to identify and distinguish Shigella spp., E. coli, and its pathotype EIEC were explored and evaluated using spectra of 456 Shigella spp., 42 E. coli, and 61 EIEC isolates. Identification with a custom-made database resulted in >94% Shigella identified at the genus level and >91% S. sonnei and S. flexneri at the species level, but the distinction of S. dysenteriae, S. boydii, and E. coli was poor. With biomarker assignment, 98% S. sonnei isolates were correctly identified, although specificity was low. Discriminating markers for S. dysenteriae, S. boydii, and E. coli were not assigned at all. Classification models using machine learning correctly identified Shigella in 96% of isolates, but most E. coli isolates were also assigned to Shigella. None of the proposed alternative approaches were suitable for clinical diagnostics for identifying Shigella spp., E. coli, and EIEC, reflecting their relatedness and taxonomical classification. We suggest the use of MALDI-TOF MS for the identification of the Shigella spp./E. coli complex, but other tests should be used for distinction.
Current Knowledge on the Oxidative-Stress-Mediated Antimicrobial Properties of Metal-Based Nanoparticles
Authors: Mammari Nour , Lamouroux Emmanuel , Boudier Ariane , Duval Raphaël E.
Published: February 2022

The emergence of multidrug-resistant (MDR) bacteria in recent years has been alarming and represents a major public health problem. The development of effective antimicrobial agents remains a key challenge. Nanotechnologies have provided opportunities for the use of nanomaterials as components in the development of antibacterial agents. Indeed, metal-based nanoparticles (NPs) show an effective role in targeting and killing bacteria via different mechanisms, such as attraction to the bacterial surface, destabilization of the bacterial cell wall and membrane, and the induction of a toxic mechanism mediated by a burst of oxidative stress (e.g., the production of reactive oxygen species (ROS)). Considering the lack of new antimicrobial drugs with novel mechanisms of action, the induction of oxidative stress represents a valuable and powerful antimicrobial strategy to fight MDR bacteria. Consequently, it is of particular interest to determine and precisely characterize whether NPs are able to induce oxidative stress in such bacteria. This highlights the particular interest that NPs represent for the development of future antibacterial drugs. Therefore, this review aims to provide an update on the latest advances in research focusing on the study and characterization of the induction of oxidative-stress-mediated antimicrobial mechanisms by metal-based NPs.
Impact of Thermal Treatment on the Starch-Protein Interplay in Red Lentils: Connecting Molecular Features and Rheological Properties
Authors: Bresciani Andrea , Emide Davide , Saitta Francesca , Fessas Dimitrios , Iametti Stefania , Barbiroli Alberto , Marti Alessandra
Published: February 2022

Thermal treatments are widely applied to gluten-free (GF) flours to change their functionality. Despite the interest in using pulses in GF formulations, the effects of thermal treatment at the molecular level and their relationship with dough rheology have not been fully addressed. Raw and heat-treated red lentils were tested for starch and protein features. Interactions with water were assessed by thermogravimetric analysis and water-holding capacity. Finally, mixing properties were investigated. The thermal treatment of red lentils induced a structural modification of both starch and proteins. In the case of starch, such changes consequently affected the kinetics of gelatinization. Flour treatment increased the temperature required for gelatinization, and led to an increased viscosity during both gelatinization and retrogradation. Regarding proteins, heat treatment promoted the formation of aggregates, mainly stabilized by hydrophobic interactions between (partially) unfolded proteins. Overall, the structural modifications of starch and proteins enhanced the hydration properties of the dough, resulting in increased consistency during mixing.
Selective Consumption of Fish Oil at End of the Day Increases the Physiological Fatty Acid Compositions of Eicosapentaenoic Acid and Docosahexaenoic Acid in Mice
Authors: Matsuzaka Hiroki , Matsuyama Hiroki , Tanaka Wataru , Tajiri Hayato , Sakakibara Hiroyuki
Published: February 2022

Diets with high daily fat consumption are associated with excess weight. However, the effects of fat type and consumption timing on excess weight remain unclear. We investigated the selection of a 30% (w/w) fat diet of soybean oil (SOY), lard (LARD), and fish oil (FISH) on the metabolic parameters of mice. Male C57BL/6 mice were divided into the double SOY-box (w-SOY), SOY-box/LARD-box (SOY-vs-LARD), or SOY-box/FISH-box (SOY-vs-FISH) groups and allowed to selectively consume for 8 weeks. The total energy intake was similar for all groups, but the mice selectively chose to consume LARD over SOY and SOY over FISH. Body weight in the SOY-vs-LARD group was significantly higher than that in the w-SOY and SOY-vs-FISH groups. Additionally, minimal but selective consumption of an omega-3 fatty-acid-rich FISH diet at the end of the active period increased the physiological fatty acid compositions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the SOY-vs-FISH group; their metabolic parameters were also lower than the SOY-vs-LARD group. In conclusion, selectively consuming small amounts of fish oil at the end of the day may prevent excess weight compared with LARD consumption.
Benefits of the Mediterranean Diet–Wine Association: The Role of Ingredients
Authors: Silva Paula , Latruffe Norbert
Published: February 2022

The cultural and nutritional aspects of the multi-secular Mediterranean civilization include diet as a central element of health and well-being, including wine if it is used in moderation [...]
A Mixture of Topical Forms of Polydeoxyribonucleotide, Vitamin C, and Niacinamide Attenuated Skin Pigmentation and Increased Skin Elasticity by Modulating Nuclear Factor Erythroid 2-like 2
Authors: Kim Hyoung Moon , Byun Kyung-A , Oh Seyeon , Yang Jin Young , Park Hyun Jun , Chung Moon Suk , Son Kuk Hui , Byun Kyunghee
Published: February 2022

It is well-known that increased oxidative stress caused by ultraviolet B (UV-B) radiation induces melanogenesis and activates metalloproteinases (MMPs), which degrade collagen and elastin fibers, leading to decreased skin elasticity. Various antioxidant agents, such as vitamin C and niacinamide, have been evaluated for use as treatments for photoaging or skin pigmentation. In this study, we evaluated the ability of a topical liquid formula of polydeoxyribonucleotide (PDRN), vitamin C, and niacinamide (PVN) delivered via a microneedling therapy system (MTS) to attenuate photoaging and pigmentation by increasing nuclear factor erythroid 2-like 2 (NRF2)/heme oxygenase-1 (HO-1) and decreasing MMP expression in a UV-B-radiated animal model. The effects of the PVN were compared with those of individual PDRN and hydroquinone (HQ) compounds. The expression of NRF2/HO-1 significantly increased in response to HQ, PDRN, and PVN in UV-B-radiated animal skin. The activity of nicotinamide adenine dinucleotide phosphate hydrogen oxidase decreased in response to HQ, PDRN, and PVN, and the superoxide dismutase activity increased. The expression of tumor protein p53 and microphthalmia-associated transcription factor and tyrosinase activity decreased in response to HQ, PDRN, and PVN, and this decrease was accompanied by decreased melanin content in the skin. The expression of nuclear factor kappa-light-chain enhancer of activated B cells and MMP2/3/9 decreased in response to HQ, PDRN, and PVN in UV-B-radiated skin. However, the expression of collagen type I α1 chain and the amount of collagen fibers that were evaluated by Masson’s trichrome staining increased in response to HQ, PDRN, and PVN. The contents of elastin fibers, fibrillin 1/2 and fibulin 5 increased in response to HQ, PDRN, and PVN. In conclusion, PVN delivered via MTS led to decreased melanogenesis and destruction of collagen and elastin fibers by MMPs, and, thus, PVN decreased skin pigmentation and increased skin elasticity.
Natural Dietary Compound Xanthohumol Regulates the Gut Microbiota and Its Metabolic Profile in a Mouse Model of Alzheimer’s Disease
Authors: Liu Wei , He Kaiwu , Wu Desheng , Zhou Li , Li Guowei , Lin Zequn , Yang Xifei , Liu Jianjun , Pui Man Hoi Maggie
Published: February 2022

Discovering new and effective drugs for the treatment of Alzheimer’s disease (AD) is a major clinical challenge. This study focuses on chemical modulation of the gut microbiome in an established murine AD model. We used the 16S rDNA sequencing technique to investigate the effect of xanthohumol (Xn) on the diversity of intestinal microflora in 2-month- and 6-month-old APP/PS1 mice, respectively. APP/PS1 and wild-type mice were treated by gavage with corn oil with or without Xn every other day for 90 days. Prior to and following treatment, animals were tested for spatial learning, cognitive and memory function. We found Xn reduced cognitive dysfunction in APP/PS1 mice and significantly regulated the composition and abundance of gut microbiota both in prevention experiments (with younger mice) and therapeutic experiments (with older mice). Differential microflora Gammaproteobacteria were significantly enriched in APP/PS1 mice treated with Xn. Nodosilineaceae and Rikenellaceae may be the specific microflora modulated by Xn. The penicillin and cephalosporin biosynthesis pathway and the atrazine degradation pathway may be the principal modulation pathways. Taken together, oral treatment with Xn may have a neuroprotective role by regulating the composition of intestinal microflora, a result that contributes to the scientific basis for a novel prophylactic and therapeutic approach to AD.
Inhibitory Effect of Ursolic Acid on the Migration and Invasion of Doxorubicin-Resistant Breast Cancer
Authors: Zong Li , Cheng Guorong , Zhao Jingwu , Zhuang Xiaoyu , Zheng Zhong , Liu Zhiqiang , Song Fengrui
Published: February 2022

The cause of death in most breast cancer patients is disease metastasis and the occurrence of multidrug resistance (MDR). Ornithine decarboxylase (ODC), which is involved into multiple pathways, is closely related to carcinogenesis and development. Ursolic acid (UA), a natural triterpenoid compound, has been shown to reverse the MDR characteristics of tumor cells. However, the effect of UA on the invasion and metastasis of tumor cells with MDR is not known. Therefore, we investigated the effects of UA on invasion and metastasis, ODC-related polyamine metabolism, and MAPK-Erk-VEGF/MMP-9 signaling pathways in a doxorubicin-resistant breast cancer cell (MCF-7/ADR) model. The obtained results showed that UA significantly inhibited the adhesion and migration of MCF-7/ADR cells, and had higher affinities with key active cavity residues of ODC compared to the known inhibitor di-fluoro-methyl-ornithine (DFMO). UA could downregulate ODC, phosphorylated Erk (P-Erk), VEGF, and matrix metalloproteinase-9 (MMP-9) activity. Meanwhile, UA significantly reduced the content of metabolites of the polyamine metabolism. Furthermore, UA increased the intracellular accumulation of Dox in MCF-7/ADR cells. Taken together, UA can inhibit against tumor progression during the treatment of breast cancer with Dox, and possibly modulate the Erk-VEGF/MMP-9 signaling pathways and polyamine metabolism by targeting ODC to exert these effects.
CoO Nanozymes with Multiple Catalytic Activities Regulate Atopic Dermatitis
Authors: Mao Mao , Guan Xuejiao , Wu Feng , Ma Lan
Published: February 2022

Herein, we prepared CoO nanozymes with three types of enzyme catalytic activities for the first time, which have SOD-like, CAT-like, and POD-like catalytic activities. This is the first study to report the preparation of CoO nanoparticles with three types of enzyme catalytic activities by the one-pot method. By modifying the surface of CoO nanozymes with a carboxyl group, its biocompatibility enhanced, so it can be used in the field of life sciences. In vitro cytotoxicity and anti-H2O2-induced ROS experiments proved that CoO nanozymes can protect HaCaT cells against ROS and cytotoxicity induced by H2O2. In addition, an atopic dermatitis (AD) mouse model was established by topical application of MC903, which verified the anti-inflammatory effect of CoO nanozymes on the AD mouse model. Traditional drugs for the treatment of AD, such as dexamethasone, have significant side-effects. The side-effects include skin burns, telangiectasias, and even serious drug dependence. CoO nano-enzymes have a low cytotoxicity and its multiple enzyme-like catalytic activities can effectively protect cells and tissues in ROS environments, which proves that CoO nano-enzymes have high application potential in the field of anti-inflammation.
Enhanced Thermoelectric Performance of Cu2Se via Nanostructure and Compositional Gradient
Authors: Bo Lin , Li Fujin , Hou Yangbo , Zuo Min , Zhao Degang
Published: February 2022

Forming co-alloying solid solutions has long been considered as an effective strategy for improving thermoelectric performance. Herein, the dense Cu2−x(MnFeNi)xSe (x = 0–0.09) with intrinsically low thermal conductivity was prepared by a melting-ball milling-hot pressing process. The influences of nanostructure and compositional gradient on the microstructure and thermoelectric properties of Cu2Se were evaluated. It was found that the thermal conductivity decreased from 1.54 Wm−1K−1 to 0.64 Wm−1K−1 at 300 K via the phonon scattering mechanisms caused by atomic disorder and nano defects. The maximum zT value for the Cu1.91(MnFeNi)0.09Se sample was 1.08 at 750 K, which was about 27% higher than that of a pristine sample.
The Impact of Restricted versus Liberal Early Fluid Volumes on Plasma Sodium, Weight Change, and Short-Term Outcomes in Extremely Preterm Infants
Authors: Diderholm Barbro , Normann Erik , Ahlsson Fredrik , Sindelar Richard , Ågren Johan
Published: February 2022

The optimal fluid requirements for extremely preterm infants are not fully known. We examined retrospectively the fluid intakes during the first week of life in two cohorts of extremely preterm infants born at 22–26 weeks of gestation before (n = 63) and after a change from a restrictive to a more liberal (n = 112) fluid volume allowance to improve nutrient provision. The cohorts were similar in gestational age and birth weight, but antenatal steroid exposure was more frequent in the second era. Although fluid management resulted in a cumulative difference in the total fluid intake over the first week of 87 mL/kg (p < 0.001), this was not reflected in a mean weight loss (14 ± 5% at a postnatal age of 4 days in both groups) or mean peak plasma sodium (142 ± 5 and 143 ± 5 mmol/L in the restrictive and liberal groups, respectively). The incidences of hypernatremia (>145 and >150 mmol/L), PDA ligation, bronchopulmonary dysplasia, and IVH were also similar. We conclude that in this cohort of extremely preterm infants a more liberal vs. a restricted fluid allowance during the first week had no clinically important influence on early changes in body weight, sodium homeostasis, or hospital morbidities.
Efficacy of Teduglutide for Parenteral Support Reduction in Patients with Short Bowel Syndrome: A Systematic Review and Meta-Analysis
Authors: Bioletto Fabio , D’Eusebio Chiara , Merlo Fabio Dario , Aimasso Umberto , Ossola Marta , Pellegrini Marianna , Ponzo Valentina , Chiarotto Alessia , De Francesco Antonella , Ghigo Ezio , Bo Simona
Published: February 2022

Teduglutide has been described as an effective treatment for parenteral support (PS) reduction in patients with short bowel syndrome (SBS). However, a quantitative summary of the available evidence is still lacking. PubMed/Medline, EMBASE, Cochrane library, OVID, and CINAHL databases were systematically searched up to July 2021 for studies reporting the rate of response (defined as a ≥20% reduction in PS) to teduglutide among PS-dependent adult patients. The rate of weaning (defined as the achievement of PS independence) was also evaluated as a secondary end-point. Ten studies were finally considered in the meta-analysis. Pooled data show a response rate of 64% at 6 months, 77% at 1 year and, 82% at ≥2 years; on the other hand, the weaning rate could be estimated as 11% at 6 months, 17% at 1 year, and 21% at ≥2 years. The presence of colon in continuity reduced the response rate (−17%, 95%CI: (−31%, −3%)), but was associated with a higher weaning rate (+16%, 95%CI: (+6%, +25%)). SBS etiology, on the contrary, was not found to be a significant predictor of these outcomes, although a nonsignificant trend towards both higher response rates (+9%, 95%CI: (−8%, +27%)) and higher weaning rates (+7%, 95%CI: (−14%, +28%)) could be observed in patients with Crohn’s disease. This was the first meta-analysis that specifically assessed the efficacy of teduglutide in adult patients with SBS. Our results provide pooled estimates of response and weaning rates over time and identify intestinal anatomy as a significant predictor of these outcomes.
Implementing the Synergy Model: A Qualitative Descriptive Study
Authors: Cordon Charissa P. , Baxter Pamela , Collerman Ari , Krull Kirsten , Aiello Celia , Lounsbury Jennifer , MacPhee Maura , Udod Sonia , Alvarado Kim , Dietrich Tim , Akhtar-Danesh Noori , Ramachandran Meena , Meisenburg Natalie
Published: February 2022

Hospitals across our nation are seeking to implement models of care that meet the primary goals of Quadruple Aim: Improved population health, cost-effective care delivery, and patient and provider satisfaction. In an effort to address the Quadruple Aim and our patients’ care needs, Hamilton Health Sciences (HHS) embarked on a model of care delivery redesign, beginning with nursing care delivery. From 2013 to 2018, 12 clinical programs at HHS implemented the Synergy Model with its accompanying synergy patient needs assessment tool for nurses to objectively assess patients’ acuity and dependency needs. Data on patients’ priority care needs were used to inform a nursing model of care redesign at HHS, including skill mix and staffing levels. This five-year project was an organization-wide quality improvement initiative. As part of the evaluation, HHS leaders partnered with health services nurse researchers to conduct a mixed methods study. This paper describes the evaluation outcomes from the qualitative component of the study, which included interviews with clinical nurse leaders and direct care nurses. Data were analyzed using descriptive thematic analysis. Some key findings were increased nurse awareness of patients’ holistic care needs and leaders’ capacity to plan staffing assignments based on patients’ priority care needs. Themes helped inform recommendations for key stakeholders, including nurse leaders and direct care nurses.
Resistin in Urine and Breast Milk: Relation to Type of Feeding and Anthropometry at 1-Month
Authors: Santosa Irena , Shoji Hiromichi , Awata Kentaro , Arai Yoshiteru , Suganuma Hiroki , Shimizu Toshiaki
Published: February 2022

Breast milk contains adipokines such as resistin and leptin and is known for its protective effect against obesity and insulin resistance. This pilot study aims to evaluate the correlation between resistin levels, feeding types (breast milk and formula), and anthropometric parameters in healthy 1-month-old term infants. Urine and breast milk samples were collected from 32 infants and their mothers at 1 month postpartum. Twelve infants were included in the breastfed group, while thirteen infants comprised the breastfed-dominant mix-fed group, and seven infants the formula-dominant mix-fed group. Using ELISA kits, we analyzed resistin levels in the infants’ urine and the mothers’ breast milk, and leptin levels in breast milk. Urinary resistin levels among the three groups were not significantly different. There was no correlation between the following: urinary resistin levels in the breastfed group with resistin levels in breast milk; resistin levels in urine with infant’s body weight and weight gain; resistin levels in breast milk with weight, age, and BMI of mothers and leptin levels in breast milk. This study suggests that the type of feeding does not affect resistin levels in term infants and resistin level does not affect growth in early infancy.
UHPLC-MS/MS Analysis of Antibiotics Transfer and Concentrations in Porcine Oral Fluid after Intramuscular Application
Authors: Gajda Anna , Nowacka-Kozak Ewelina , Gbylik-Sikorska Małgorzata , Cybulski Piotr
Published: February 2022

The monitoring of antibiotic use in animals is a crucial element to ensure food safety. The main goal of this study was to analyse the distribution of selected antibiotics to porcine oral fluid, as well as to demonstrate that an oral fluid is an alternative to other biological matrices used in the control of antibacterials. Therefore, an animal study with pigs treated using seven different antibiotics was performed. Sulfadoxine (SDX) with trimethoprim (TRMP), lincomycin (LIN), tiamulin (TIAM), tylosin (TYL), amoxicillin (AMX) and penicillin G (PEN G) were injected intramuscularly to pigs, and concentrations of these analytes in the oral fluid were assessed. Ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) was used to quantify the analytes. On the first day of medication, the highest concentrations for SDX and TRMP at the level of 22,300 µg/kg and 14,100 µg/kg were found, respectively. The concentrations of LIN (10,500 µg/kg) and TIAM (7600 µg/kg) were also relatively high. The peak of TYL was recorded on the second day of drug administration. Most of the analytes were present in oral fluid for 30 days, apart from TYL, which was detected for 27 days. It was found that AMX and PEN G were quantified only for 5 and 8 days, respectively, at very low concentrations. It was found that oral fluid can be used for the verification of antibiotics on pig farms.
Design, Molecular Docking, Synthesis, Anticancer and Anti-Hyperglycemic Assessments of Thiazolidine-2,4-diones Bearing Sulfonylthiourea Moieties as Potent VEGFR-2 Inhibitors and PPARγ Agonists
Authors: Abdelgawad Mohamed A. , El-Adl Khaled , El-Hddad Sanadelaslam S. A. , Elhady Mostafa M. , Saleh Nashwa M. , Khalifa Mohamed M. , Khedr Fathalla , Alswah Mohamed , Nayl AbdElAziz A. , Ghoneim Mohammed M. , Abd El-Sattar Nour E. A.
Published: February 2022

Newly designed thiazolidine-2,4-diones 3–7a–c were synthesized, and their anticancer activities were screened against three cancer lines. They showed potent activities against HepG2 compared to the other HCT116 and MCF-7 tumor cell lines. Compounds 7c and 6c were detected as highly effective derivatives against MCF-7 (IC50 = 7.78 and 8.15 µM), HCT116 (IC50 = 5.77 and 7.11 µM) and HepG2 (IC50 = 8.82 and 8.99 µM). The highly effective derivatives 6a–c and 7a–c were tested against VERO normal cell lines. All derivatives were evaluated for their VEGFR-2 inhibitory actions and demonstrated high to low activities, with IC50 values varying from 0.08 to 0.93 µM. Moreover, derivatives 5a–c, 6a–c and 7a–c were assessed to verify their in vitro binding affinities to PPARγ and insulin-secreting activities. Finally, docking studies were performed to explore their affinities and binding modes toward both VEGFR-2 and PPARγ receptors.
Metabolic Circuits in Sap Extracts Reflect the Effects of a Microbial Biostimulant on Maize Metabolism under Drought Conditions
Authors: Othibeng Kgalaletso , Nephali Lerato , Myoli Akhona , Buthelezi Nombuso , Jonker Willem , Huyser Johan , Tugizimana Fidele
Published: February 2022

The use of microbial biostimulants in the agricultural sector is increasingly gaining momentum and drawing scientific attention to decode the molecular interactions between the biostimulants and plants. Although these biostimulants have been shown to improve plant health and development, the underlying molecular phenomenology remains enigmatic. Thus, this study is a metabolomics work to unravel metabolic circuits in sap extracts from maize plants treated with a microbial biostimulant, under normal and drought conditions. The biostimulant, which was a consortium of different Bacilli strains, was applied at the planting stage, followed by drought stress application. The maize sap extracts were collected at 5 weeks after emergence, and the extracted metabolites were analyzed on liquid chromatography-mass spectrometry platforms. The acquired data were mined using chemometrics and bioinformatics tools. The results showed that under both well-watered and drought stress conditions, the application of the biostimulant led to differential changes in the profiles of amino acids, hormones, TCA intermediates, phenolics, steviol glycosides and oxylipins. These metabolic changes spanned several biological pathways and involved a high correlation of the biochemical as well as structural metabolic relationships that coordinate the maize metabolism. The hypothetical model, postulated from this study, describes metabolic events induced by the microbial biostimulant for growth promotion and enhanced defences. Such understanding of biostimulant-induced changes in maize sap pinpoints to the biochemistry and molecular mechanisms that govern the biostimulant–plant interactions, which contribute to ongoing efforts to generate actionable knowledge of the molecular and physiological mechanisms that define modes of action of biostimulants.
Evaluation of Reference Genes in the Polyploid Complex Dianthus broteri (Caryophyllaceae) Using qPCR
Authors: Rodríguez-Parra Alba , Picazo-Aragonés Jesús , Balao Francisco
Published: February 2022

Dianthus broteri is an endemic complex which is considered the largest polyploid series within the Dianthus genus. This polyploid species involves four cytotypes (2×, 4×, 6× and 12×) with spatial and ecological segregation. The study of gene expression in polyploid species must be very rigorous because of the effects of duplications on gene regulation. In these cases, real-time polymerase chain reaction (qPCR) is the most appropriate technique for determining the gene expression profile because of its high sensitivity. The relative quantification strategy using qPCR requires genes with stable expression, known as reference genes, for normalization. In this work, we evaluated the stability of 13 candidate genes to be considered reference genes in leaf and petal tissues in Dianthus broteri. Several statistical analyses were used to determine the most stable candidate genes: Bayesian analysis, network analysis based on equivalence tests, geNorm and BestKeeper algorithms. In the leaf tissue, the most stable candidate genes were TIP41, TIF5A, PP2A and SAMDC. Similarly, the most adequate reference genes were H3.1, TIP41, TIF5A and ACT7 in the petal tissue. Therefore, we suggest that the best reference genes to compare different ploidy levels for both tissues in D. broteri are TIP41 and TIF5A.