bluuebunny/crossref_metadata_2025 huggingface dataset:
Personal Correlates of Support for Medical and Recreational Cannabis Legalization in Australia
Authors: Chiu Vivian , Chan Gary , Hall Wayne , Hides Leanne , Lim Carmen , Leung Janni
Published: February 2021

Introduction and Aims: Increasingly more Australians are in favor of legalizing medical and recreational cannabis use. This paper explored the personal characteristics of those who supported each of these policies in Australia.

Design: Cross-sectional national survey.

Methods: This study included 21,729 participants aged 18 years and above who responded to the 2016 National Drug Strategy Household Survey. Participants were provided the assurance of confidentiality for their participations. Logistic regression models were used to examine the relationships between personal characteristics and support for the legalization of medical and recreational cannabis.

Results: Overall, 77 and 40% of participants supported the legalization of medical and recreational cannabis respectively. People of older age were more likely to support medical cannabis legalization while those who supported legalization of recreational cannabis use were more likely to be younger. Medical cannabis supporters were more likely to report chronic pain (OR = 1.44, 95% CI: 1.04, 2.00) while recreational cannabis supporters were more likely to suffer high level of psychological distress (OR = 1.28, 95% CI: 1.14, 1.43). Experience with cannabis use was strongly associated with supportive attitudes, with recent cannabis users almost 14 times (OR = 14.13, 95% CI: 5.37, 37.20) and 34 times (OR = 33.74, 95% CI: 24.22, 47.01) more likely to support the legalization of medical and recreational cannabis use, respectively.

Discussion and Conclusions: The majority of Australians approve the legalization of cannabis for medicinal purposes but most remain cautious about legalizing recreational cannabis use. The sociodemographic and clinical profile of supporters of medical and recreational legalization suggests a potential interaction of self-interests and beliefs about the harms of cannabis use.
Protein Biomarkers of Autism Spectrum Disorder Identified by Computational and Experimental Methods
Authors: Yao Fang , Zhang Kaoyuan , Feng Chengyun , Gao Yan , Shen Liming , Liu Xukun , Ni Jiazuan
Published: February 2021

Background: Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder that affects millions of people worldwide. However, there are currently no reliable biomarkers for ASD diagnosis.

Materials and Methods: The strategy of computational prediction combined with experimental verification was used to identify blood protein biomarkers for ASD. First, brain tissue–based transcriptome data of ASD were collected from Gene Expression Omnibus database and analyzed to find ASD-related genes by bioinformatics method of significance analysis of microarrays. Then, a prediction program of blood-secretory proteins was applied on these genes to predict ASD-related proteins in blood. Furthermore, ELISA was used to verify these proteins in plasma samples of ASD patients.

Results: A total of 364 genes were identified differentially expressed in brain tissue of ASD, among which 59 genes were predicted to encode ASD-related blood-secretory proteins. After functional analysis and literature survey, six proteins were chosen for experimental verification and five were successfully validated. Receiver operating characteristic curve analyses showed that the area under the curve of SLC25A12, LIMK1, and RARS was larger than 0.85, indicating that they are more powerful in discriminating ASD cases from controls.

Conclusion: SLC25A12, LIMK1, and RARS might serve as new potential blood protein biomarkers for ASD. Our findings provide new insights into the pathogenesis and diagnosis of ASD.
Demographic, Health, and Exposure Risks Associated With Cognitive Loss, Alzheimer's Disease and Other Dementias in US Military Veterans
Authors: Zhu Carolyn W. , Sano Mary
Published: February 2021

The US military veteran population receiving care through the Veterans Health Administration (VHA) is particularly susceptible to cognitive impairment and dementias such as Alzheimer's disease and related dementias due to demographic, clinical, and economic factors. In this report we summarize the prevalence of dementia among US veterans and risks associated with AD and related dementias. We discuss the likelihood that these risks may be increasing in those about to enter the age in which dementias are common. We propose that VHA, the largest integrated health care system in the US, has shown promise in managing health risks that impact dementia prevention and propose further system wide approaches to be assessed for effective dementia prevention and care delivery.
The Complex Interaction Between the Major Sleep Symptoms, the Severity of Obstructive Sleep Apnea, and Sleep Quality
Authors: Frangopoulos Frangiskos , Zannetos Savvas , Nicolaou Ivi , Economou Nicholas-Tiberio , Adamide Tonia , Georgiou Andreas , Nikolaidis Pantelis T. , Rosemann Thomas , Knechtle Beat , Trakada Georgia
Published: February 2021

Introduction: Little information exists in the general population whether clinical presentation phenotypes of obstructive sleep apnea (OSA) differ in terms of sleep quality and comorbidities.

Aim: The purpose of our study was to assess possible differences between symptomatic and asymptomatic OSA patients concerning syndrome's severity, patients' sleep quality, and comorbidities.

Subjects and methods: First, in a nationwide, stratified, epidemiological survey, 4,118 Cypriot adult participants were interviewed about sleep habits and complaints. In the second stage of the survey, 264 randomly selected adults underwent a type III sleep study for possible OSA. Additionally, they completed the Greek version of Pittsburgh Sleep Quality Index (Gr-PSQI), Epworth Sleepiness Scale (ESS), Athens Insomnia Scale (AIS), and Hospital Anxiety and Depression Scale (HADS).

Results: From 264 enrolled participants, 155 individuals (40 females and 115 males) were first diagnosed with OSA. Among these 155 patients, 34% had ESS ≥ 10 and 49% AIS ≥ 6. One or both symptoms present categorized the individual as symptomatic (60%) and neither major symptom as asymptomatic (40%). There were no significant statistical differences (SSDs) between the two groups (symptomatic–asymptomatic) with regard to anthropometrics [age or gender; neck, abdomen, and hip circumferences; and body mass index (BMI)]. The two groups had no differences in OSA severity—as expressed by apnea–hypopnea index (AHI), oxygen desaturation index (ODI), and mean oxyhemoglobin saturation (SaO2)—and in cardiometabolic comorbidities. Symptomatic patients expressed anxiety and depression more often than asymptomatics (p < 0.001) and had poorer subjective sleep quality (Gr-PSQI, p < 0.001). According to PSQI questionnaire, there were no SSDs regarding hours in bed and the use of sleep medications, but there were significant differences in the subjective perception of sleep quality (p < 0.001), sleep efficiency (p < 0.001), duration of sleep (p = 0.001), sleep latency (p = 0.007), daytime dysfunction (p < 0.001), and finally sleep disturbances (p < 0.001).

Conclusion: According to our data, OSA patients reporting insomnia-like symptoms and/or sleepiness do not represent a more severe phenotype, by the classic definition of OSA, but their subjective sleep quality is compromised, causing a vicious cycle of anxiety or depression.
Monitoring of Adverse Drug Reaction-Related Parameters in Children and Adolescents Treated With Antipsychotic Drugs in Psychiatric Outpatient Clinics
Authors: Minjon Lenneke , Brozina Ivona , Egberts Toine C. G. , Heerdink Eibert R. , van den Ban Els
Published: February 2021

Aim: To assess the frequency of monitoring of adverse drug reaction (ADR) related parameters in children and adolescents treated with antipsychotic drugs in psychiatric outpatient clinics and the considerations when monitoring was not performed.

Methods: This retrospective follow-up study included 100 randomly selected outpatients aged ≤18 years who had a first prescription of an antipsychotic drug recorded in the electronic medical records of psychiatric outpatient clinics between 2014 and 2017. They were followed for up to 3 years. This study assessed the frequency of monitoring for physical parameters (weight, height, body mass index, waist circumference, pulse, blood pressure, and an electrocardiogram) and laboratory parameters (glucose, lipids, and prolactin) before the first prescription of an antipsychotic drug as well as during its use. Monitoring frequencies were stratified by the patient characteristics (sex, age, cardiovascular risk factors, and use of other psychotropic drugs), and by location of antipsychotic drug initiation (psychiatric outpatient clinic or elsewhere). Additionally, this study assessed the considerations mentioned in the medical records for not monitoring ADR-related parameters.

Results: Overall, physical parameters were monitored more frequently (weight: 85.9% during the first half-year) than laboratory parameters (glucose and cholesterol: both 23.5%). There were no significant differences in monitoring at least one physical as well as in monitoring at least one laboratory parameter during the baseline period and during the total follow-up of antipsychotic drug treatment between the patient characteristics. In total, 3% of the children and adolescents were never monitored for any physical parameter, and 54% were never monitored for any laboratory parameter. For a minority of the children (14.8%) who were never monitored for laboratory parameters, considerations were recorded in their medical records, including refusal by the child or parents and monitoring performed by the general practitioner or elsewhere.

Conclusion: Monitoring frequencies of ADR-related parameters in children and adolescents treated with antipsychotic drugs in psychiatric outpatient clinics varied and especially monitoring of laboratory parameters was infrequent. Considerations why monitoring was not performed were rarely recorded. The optimal method of monitoring and documentation thereof should become clear to optimize the benefit-risk balance of antipsychotic drug treatment for each child.
Discharge Body Mass Index, Not Illness Chronicity, Predicts 6-Month Weight Outcome in Patients Hospitalized With Anorexia Nervosa
Authors: Redgrave Graham W. , Schreyer Colleen C. , Coughlin Janelle W. , Fischer Laura K. , Pletch Allisyn , Guarda Angela S.
Published: February 2021

Proposed treatments for severe and enduring anorexia nervosa (SE-AN) focus on quality of life, and psychological and social functioning. By de-emphasizing weight restoration as a priority, however, premature diagnosis of SE-AN may reduce potential for recovery. The present study assessed the effect of weight restoration, illness duration, and severity on treatment outcome 6 months after discharge from an intensive, meal-based behavioral treatment program. Participants included hospitalized adult women (N = 191) with AN or underweight other specified feeding and eating disorder (OSFED). Participants were characterized as short-term (ill <7 years; n = 74) or long-term ill (ill ≥ 7 years; n = 117). Compared with short-term ill, long-term ill patients were older, had lower lifetime body mass index (BMI), more prior admissions, and exhibited greater depression and neuroticism. Long-term vs. short-term ill patients gained weight at the same rate (~2 kg/wk) and were equally likely to be weight restored by discharge (>75% reached BMI ≥ 19 kg/m2 in both groups). At 6-month follow-up (n = 99), both groups had equivalent self-reported BMI, and depression, drive for thinness, body dissatisfaction, and bulimia scores. The only predictor of BMI ≥ 19 kg/m2 at follow-up was discharge BMI. The likelihood of a BMI ≥ 19 kg/m2 at follow-up was 5-fold higher for those with discharge BMI ≥ 19 kg/m2. Few studies of long-term ill inpatients with AN have examined the impact of full weight restoration on short-term outcomes. This study supports the therapeutically optimistic stance that, regardless of illness duration, hospitalized patients with AN benefit from gaining weight to a BMI ≥ 19 kg/m2.
Last Mile Access to Enriched Children's Complementary Food: Mitigating Malnutrition in Kenya
Authors: Pamela Muthuuri , Joseph Kassim , Patrick Richard , Florence Mwarania
Published: February 2021

Commercial complementary foods are not accessible at the last mile of delivery, despite a veritable stunted growth explosion in Kenya. A Mile for the Brain aims to reduce child malnutrition by solving the pervasive distribution bottlenecks and prohibitive pricing challenges. This paper presents the systematized measurement for change process. We focus on the selection of off-the-shelf complementary foods, training of women entrepreneurs responsible for commercializing these complementary foods, coaching given to mothers on appropriate feeding education, and lastly the learning cycle revolving around feeding mothers, entrepreneurs for the Mile for the Brain social enterprise itself. We highlight the real-life challenges involved in this process in the context of adversity and constrained resources. The results, findings, and policy implications of this study will be reported elsewhere.
Emergence of NDM-5 Producing Carbapenem-Resistant Klebsiella aerogenes in a Pediatric Hospital in Shanghai, China
Authors: Pan Fen , Xu Qi , Zhang Hong
Published: February 2021

Background: Carbapenem-resistant Klebsiella aerogenes (CRKA) has posed a serious threat for clinical anti-infective therapy. However, the molecular characteristics of CRKA in Shanghai are rarely reported.

Objective: This study aimed to investigate the resistance profiles, dissemination mechanism, and molecular characteristics of CRKA strains isolated from children in a pediatric hospital, Shanghai.

Method: Fifty CRKA isolates were collected in 2019. Antimicrobial susceptibility of the strains was determined by broth microdilution method. The β-lactamases and outer membrane porin genes were characterized by polymerase chain reaction (PCR). Conjugation experiments were performed to determine the transferability of the plasmids. The plasmids were typed based on their incompatibility group using the PCR-based replicon typing method. Multilocus sequence typing (MLST) and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) were performed for the genetic relationship.

Results: All CRKA strains showed high level of resistance to cephalosporins and carbapenems, but still susceptible to aminoglycosides, colistin, and tigecycline. Forty five of fifty isolates carried blaNDM−5 genes (45/50, 90%), alongside with other β-Lactamase genes including blaCTX−M−1, blaTEM−1, and blaSHV−11 being detected. Loss of ompK35 and ompK36 genes were observed in 14% (7/50) and 28% (14/50), respectively, with 5 isolates lacking both ompK35 and ompK36. MLST analysis demonstrated that the majority of isolates belonged to ST4 (47/50, 94%) and ERIC-PCR fingerprinting was performed to identify NDM-5-producing isolates with approximately or more than 80% similarity levels. Plasmids carrying blaNDM−5 were successfully transferred to the E. coli recipient and plasmid typing showed that IncX3 were the prevalent among CRKA isolates.

Conclusions: Our finding revealed the emergence of NDM-5 producing CRKA belonging to ST4 among children in Shanghai. Further attention should be paid to control the horizontal spread of the Class B carbapenemases like NDM in children.
A National Study of Colorectal Cancer Survivorship Disparities: A Latent Class Analysis Using SEER (Surveillance, Epidemiology, and End Results) Registries
Authors: Montiel Ishino Francisco A. , Odame Emmanuel A. , Villalobos Kevin , Liu Xiaohui , Salmeron Bonita , Mamudu Hadii , Williams Faustine
Published: February 2021

Introduction: Long–standing disparities in colorectal cancer (CRC) outcomes and survival between Whites and Blacks have been observed. A person–centered approach using latent class analysis (LCA) is a novel methodology to assess and address CRC health disparities. LCA can overcome statistical challenges from subgroup analyses that would normally impede variable–centered analyses like regression. Aim was to identify risk profiles and differences in malignant CRC survivorship outcomes.

Methods: We conducted an LCA on the Surveillance, Epidemiology, and End Results data from 1975 to 2016 for adults ≥18 (N = 525,245). Sociodemographics used were age, sex/gender, marital status, race, and ethnicity (Hispanic/Latinos) and stage at diagnosis. To select the best fitting model, we employed a comparative approach comparing sample-size adjusted BIC and entropy; which indicates a good separation of classes.

Results: A four–class solution with an entropy of 0.72 was identified as: lowest survivorship, medium-low, medium-high, and highest survivorship. The lowest survivorship class (26% of sample) with a mean survival rate of 53 months had the highest conditional probabilities of being 76–85 years–old at diagnosis, female, widowed, and non-Hispanic White, with a high likelihood with localized staging. The highest survivorship class (53% of sample) with a mean survival rate of 92 months had the highest likelihood of being married, male with localized staging, and a high likelihood of being non-Hispanic White.

Conclusion: The use of a person–centered measure with population-based cancer registries data can help better detect cancer risk subgroups that may otherwise be overlooked.
A Survey on Joint Communication-Radar Systems
Authors: Mazahir Sana , Ahmed Sajid , Alouini Mohamed-Slim
Published: February 2021

Concerns for spectrum congestion have spurred extensive research efforts on efficient spectrum management. Therefore, devising schemes for spectrum sharing between radar and wireless communication systems has become an important area of research. Joint communications-radar (JCR) systems are among the several approaches proposed to achieve this objective. In JCR systems, additional components and processes are added to an existing standardized communication platform to enable radar functions. Moreover, the communication waveform is used as an integrated JCR waveform, i.e., the same signal is used to communicate information to a receiver and to perform radar detection and estimation operations for a nearby target. The most common application of JCR systems is found in vehicle-to-vehicle (V2V) communication scenarios. In this article, an overview of the spectrum sharing methods is presented, with a focus on JCR systems in automotive and other applications. We first review the recent works on IEEE 802.11p- and IEEE 802.11ad-based radars. A basic description of the modeling of a JCR system and channels is presented, followed by discussions on the main components and processes employed in various JCR systems. We are mainly interested in how radar detection and estimation functions are performed in conjunction with the communication receiver functions with minimal alterations to the existing system. At the end of the paper, some performance trade-offs between the communication and radar sub-systems are also discussed.
Guidelines for Robotic Flexible Endoscopy at the Time of COVID-19
Authors: Onaizah Onaizah , Koszowska Zaneta , Winters Conchubhair , Subramanian Venkatamaran , Jayne David , Arezzo Alberto , Obstein Keith L. , Valdastri Pietro
Published: February 2021

Flexible endoscopy involves the insertion of a long narrow flexible tube into the body for diagnostic and therapeutic procedures. In the gastrointestinal (GI) tract, flexible endoscopy plays a major role in cancer screening, surveillance, and treatment programs. As a result of gas insufflation during the procedure, both upper and lower GI endoscopy procedures have been classified as aerosol generating by the guidelines issued by the respective societies during the COVID-19 pandemic—although no quantifiable data on aerosol generation currently exists. Due to the risk of COVID-19 transmission to healthcare workers, most societies halted non-emergency and diagnostic procedures during the lockdown. The long-term implications of stoppage in cancer diagnoses and treatment is predicted to lead to a large increase in preventable deaths. Robotics may play a major role in this field by allowing healthcare operators to control the flexible endoscope from a safe distance and pave a path for protecting healthcare workers through minimizing the risk of virus transmission without reducing diagnostic and therapeutic capacities. This review focuses on the needs and challenges associated with the design of robotic flexible endoscopes for use during a pandemic. The authors propose that a few minor changes to existing platforms or considerations for platforms in development could lead to significant benefits for use during infection control scenarios.
We Are the Earth and the Earth Is Us: How Palates Link Foodscapes, Landscapes, Heartscapes, and Thoughtscapes
Authors: Provenza Frederick D. , Anderson Cindi , Gregorini Pablo
Published: February 2021

Humans are participating in the sixth mass extinction, and for the first time in 200,000 years, our species may be on the brink of extinction. We are facing the greatest challenges we have ever encountered, namely how to nourish eight billion people in the face of changing climates ecologically, diminish disparity between the haves and the have-nots economically, and ease xenophobia, fear, and hatred socially? Historically, our tribal nature served us well, but the costs of tribalism are now far too great for one people inhabiting one tiny orb. If we hope to survive, we must mend the divides that isolate us from one another and the communities we inhabit. While not doing so could be our undoing, doing so could transform our collective consciousness into one that respects, nourishes, and embraces our interdependence with life on Earth. At a basic level, we can cultivate life by using nature as a model for how to produce and consume food; by decreasing our dependence on fossil fuels for energy to grow, process, and transport food; and by transcending persistent battles over one-size-fits-all plant- or animal-based diets. If we learn to do so in ways that nourish life, we may awaken individually and collectively to the wisdom of the Maori proverb Ko au te whenua. Ko te whenua Ko au: I am the land. The land is me. In this paper, we use “scapes” —foodscapes, landscapes, heartscapes, and thoughtscapes—as unifying themes to discuss our linkages with communities. We begin by considering how palates link animals with foodscapes. Next, we address how palates link foodscapes with landscapes. We then consider how, through our reverence for life, heartscapes link palates with foodscapes and landscapes. We conclude with transformations of thoughtscapes needed to appreciate life on Earth as a community to which we belong, rather than as a commodity that belongs to us.
Impact of Cryopreservation on Spermatozoa Freeze-Thawed Traits and Relevance OMICS to Assess Sperm Cryo-Tolerance in Farm Animals
Authors: Khan Ibrar Muhammad , Cao Zubing , Liu Hongyu , Khan Adnan , Rahman Sajid Ur , Khan Muhammad Zahoor , Sathanawongs Anucha , Zhang Yunhai
Published: February 2021

Sperm cryopreservation is a powerful tool for the livestock breeding program. Several technical attempts have been made to enhance the efficiency of spermatozoa cryopreservation in different farm animal species. However, it is well-recognized that mammalian spermatozoa are susceptible to cryo-injury caused by cryopreservation processes. Moreover, the factors leading to cryo-injuries are complicated, and the cryo-damage mechanism has not been methodically explained until now, which directly influences the quality of frozen–thawed spermatozoa. Currently, the various OMICS technologies in sperm cryo-biology have been conducted, particularly proteomics and transcriptomics studies. It has contributed while exploring the molecular alterations caused by cryopreservation, identification of various freezability markers and specific proteins that could be added to semen diluents before cryopreservation to improve sperm cryo-survival. Therefore, understanding the cryo-injury mechanism of spermatozoa is essential for the optimization of current cryopreservation processes. Recently, the application of newly-emerged proteomics and transcriptomics technologies to study the effects of cryopreservation on sperm is becoming a hotspot. This review detailed an updated overview of OMICS elements involved in sperm cryo-tolerance and freeze-thawed quality. While also detailed a mechanism of sperm cryo-injury and utilizing OMICS technology that assesses the sperm freezability potential biomarkers as well as the accurate classification between the excellent and poor freezer breeding candidate.
Detection and Genetic Diversity of Porcine Coronavirus Involved in Diarrhea Outbreaks in Spain
Authors: Puente Héctor , Argüello Héctor , Mencía-Ares Óscar , Gómez-García Manuel , Rubio Pedro , Carvajal Ana
Published: February 2021

Porcine enteric coronaviruses include some of the most relevant viral pathogens to the swine industry such as porcine epidemic diarrhea virus (PEDV) or porcine transmissible gastroenteritis virus (TGEV) as well as several recently identified virus such as swine enteric coronavirus (SeCoV), porcine deltacoronavirus (PDCoV) or swine enteric alphacoronavirus (SeACoV). The aim of this study is the identification and characterization of enteric coronaviruses on Spanish pig farms between 2017 and 2019. The study was carried out on 106 swine farms with diarrhea outbreaks where a viral etiology was suspected by using two duplex RT-PCRs developed for the detection of porcine enteric coronaviruses. PEDV was the only coronavirus detected in our research (38.7% positive outbreaks, 41 out of 106) and neither TGEV, SeCoV, PDCoV nor SeACoV were detected in any of the samples. The complete S-gene of all the PEDV isolates recovered were obtained and compared to PEDV and SeCoV sequences available in GenBank. The phylogenetic tree showed that only PEDV of the INDEL 2 or G1b genogroup has circulated in Spain between 2017 and 2019. Three different variants were detected, the recombinant PEDV-SeCoV being the most widespread. These results show that PEDV is a relevant cause of enteric disorders in pigs in Spain while new emerging coronavirus have not been detected so far. However, the monitoring of these virus is advisable to curtail their emergence and spread.
Systematic Investigations of the ZF-HD Gene Family in Tobacco Reveal Their Multiple Roles in Abiotic Stresses
Authors: Sun Jinhao , Xie Minmin , Li Xiaoxu , Li Zhiyuan , Wang Qi , Ding Anming , Wang Weifeng , Sun Yuhe
Published: February 2021

Zinc finger homeodomain (ZF-HD) transcription factors play significant roles in plant growth and responses to environmental stresses. In this study, 32 ZF-HD genes identified in the tobacco (Nicotiana tabacum L.) genome were divided into six groups according to phylogenetic analysis with Arabidopsis and tomato ZF-HD members. An examination of gene structures and conserved motifs revealed the relatively conserved exon/intron structures and motif organization within each subgroup. In addition, various stress-related elements are found in the promoter region of these genes. The expression profiling analysis revealed that NtZF-HD genes expressed in different tissues and could be induced by several abiotic stresses. Notably, NtZF-HD21 was highly expressed in response to the drought treatments. Subcellular localization analysis and a virus-induced gene silencing (VIGS) experiment were performed to investigate the potential functions of NtZF-HD21. The subcellular localization indicated that NtZF-HD21 is a nuclear protein. Furthermore, gene silencing of the NtZF-HD21 gene reduced the drought resistance of tobacco. These findings provide insights for further biological functional analyses of the NtZF-HD genes in tobacco.
Mycobiome Composition and Diversity under the Long-Term Application of Spent Mushroom Substrate and Chicken Manure
Authors: Frąc Magdalena , Pertile Giorgia , Panek Jacek , Gryta Agata , Oszust Karolina , Lipiec Jerzy , Usowicz Bogusław
Published: February 2021

Waste exogenous organic matter, including spent mushroom substrate (SMS) and chicken manure (CM), can be used as the basis of a soil-improving cropping system in sustainable agriculture. However, there is—as yet—a lack of information about important quality indicators such as the fungal community relative abundance, structure and biodiversity in soils treated with these additives. In this study, the responses of the soil fungal community composition and mycobiome diversity to SMS and CM application compared to the control soil were evaluated using a combination of the following molecular approaches: quantitative polymerase chain reactions, denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, and next-generation sequencing. The most abundant phylum for both treatments was Ascomycota, followed by Basidiomycota. The application of SMS and CM increased the abundance of fungi, including Tremellomycetes and Pezizomycetes for the SMS additive, while the Mortierellomycetes, Pezizomycetes, and Leotiomycetes levels increased after CM addition. SMS and CM beneficially reduced the relative abundance of several operational taxonomic units (OTUs) which are potential crop pathogens. The results provide a novel insight into the fungal community associated with organic additives, which should be beneficial in the task of managing the soil mycobiome as well as crop protection and productivity.
Impact of Grass Cover Management with Herbicides on Biodiversity, Soil Cover and Humidity in Olive Groves in the Southern Iberian
Authors: Piñar Fuentes J.C. , Leiva Felipe , Cano-Ortiz Ana , Musarella Carmelo M. , Quinto-Canas Ricardo , Pinto-Gomes Carlos J. , Cano Eusebio
Published: February 2021

In the present work, we studied the effect of herbicide use on extensive olive grove cultivation. To carry out this study, we analysed the effect that herbicide use had on biodiversity, vegetation cover and soil water content. For this purpose, 96 vegetation and soil sampling points were first taken, then georeferenced, and for each sampling point, several bioclimatic variables were interpolated. We concluded that the management of cover crops with herbicides over a long period of time resulted in a decrease in biodiversity, and the dominance of some species that were more resistant to herbicides was increased. Another finding was that the vegetation cover was reduced in the resampling in cases with herbicide management and that the location within the cropland (under the tree canopy, road, boundary or pasture) also has an influence. Finally, the study of soil moisture shows that soil water content was lower in the case of management with herbicides than in the case of management without herbicides. This loss of soil moisture was more accentuated and faster in areas with less vegetation cover. This work highlights the need to change the management models for tree crops in order to preserve biodiversity, soil quality and optimise water resources in a context of accelerated climate change in one of the regions most severely affected by global warming, the Mediterranean belt.
Constitutive and Induced Salt Tolerance Mechanisms and Potential Uses of Limonium Mill. Species
Authors: González-Orenga Sara , Grigore Marius-Nicusor , Boscaiu Monica , Vicente Oscar
Published: February 2021

Limonium is one of the most interesting and biodiverse genera of halophytes, with many species adapted to saline environments. Limonium species have a promising potential as cultivated minor crops as many have ornamental value, or are already used as medicinal plants. Other species are marketed as gourmet food or can be used for decontamination of polluted soils. Design and implementation of specific breeding programmes are needed to fully realise this potential, based on the vast genetic variation and high stress tolerance of wild species within the genus. Most Limonium species are halophytes, but many are also resistant to drought, especially those from the Mediterranean and other arid regions. Such species constitute attractive models for basic research on the mechanisms of stress tolerance, both constitutive and induced. As typical recretohalopyhtes, with excretive salt glands, Limonium species possess remarkable morpho-anatomical traits. Salt tolerance in this genus relies also on ion accumulation in the leaves, the concomitant use of diverse osmolytes for osmotic adjustment, and the activation of efficient antioxidant systems.
Evaluation of Various Nitrogen Indices in N-Fertilizers with Inhibitors in Field Crops: A Review
Authors: Folina Antigolena , Tataridas Alexandros , Mavroeidis Antonios , Kousta Angeliki , Katsenios Nikolaos , Efthimiadou Aspasia , Travlos Ilias S. , Roussis Ioannis , Darawsheh Mohammed K. , Papastylianou Panagiota , Kakabouki Ioanna
Published: February 2021

Nitrogen (N) management remains a global challenge for the sustainability of diversified farming systems. Field crops are often over-supplied with nitrogen by farmers aiming to high productivity. Although the increase of nitrogen rates leads in many instances to high yields, degree of effectiveness for nitrogen use remains low. Urease and nitrification inhibitors are technologies which have been present in the fertilizers market at least 50 years. Inhibitors exploitation ensures long-term nitrogen release and improved N-uptake by plants and N-storage in seeds and silage. Avail of inhibitors, such as the decline of nitrogen leaching in form of NO3−, reduction of emissions in NH3 form, and rise of yield, are some of the desirable attributes that are derived from their integration in fertilization schedules. This review reports the evaluation of applied nitrogen, with inhibitors, and field crops based on nitrogen indices. The examined N-indicators include Nitrogen use efficiency (NUE), Nitrogen Utilization Efficiency (NUtE,) Nitrogen Agronomic Efficiency (NAE), Nitrogen Harvest Index (NHI), and N uptake. This review gathered all, to the best of our knowledge, available data regarding the utilization of nitrification and urease inhibitors under an exclusively agronomic perspective. Either dual or single use of nitrification and urease inhibitors has been reported to significantly increase yield components and promote nitrogen uptake. To conclude, the assessment of N-related indices is vital to promoting sustainability in diversified farming systems, while the integration of inhibitors in national N fertilizations schemes may contribute to system profitability through enhancement of N-supply to crops.
Nitrogen Gap Amelioration Is a Core for Sustainable Intensification of Agriculture—A Concept
Authors: Grzebisz Witold , Łukowiak Remigiusz
Published: February 2021

The main reason for the development of the yield gap in crop production is the inefficient management of nitrogen (N). The nitrogen gap (NG) cannot be ameliorated without an indication and quantification of soil characteristics that limit N uptake by a crop plant. The insufficient supply of N to a plant during its cardinal stages of yield formation is a result of two major-variabilities. The first is spatial variability in the soil characteristics responsible for water supply to a plant, also serving as a nutrient carrier. The second is a vertical variability in soil factors, decisive for pools of available nutrients, and their in-season accessibility to the grown crop. The long-term strategy for NG cover should focus first on soil characteristics (humus stock, pH, nutrient content) responsible for water storage and its availability to the currently grown plant. Diagnostics of plant nutrient availability should deliver data on their contents both in the topsoil and subsoil. The combined use of both classical diagnostic tools and spectral imagery is a way to divide a single field into units, differing in productivity. Remote-sensing techniques offer a broad number of tools to define the in-season crop canopy requirement for fertilizer N in homogenous field units.
Long-Term Analysis of the Variability of Agronomic Characters in the VIR Oat Germplasm Collection in Central Black Soil Region of Russia
Authors: Loskutov Igor G. , Novikova Liubov Yu. , Belskaya Galina V. , Blinova Elena V.
Published: February 2021

Climate change has become a significant factor in crop production in the 21st century for many countries. To turn losses into profit, adaptation measures are needed, which are based on the analysis and forecast of economically valuable characteristics of crops. The field trial data were analyzed for 764 oat accessions from the global germplasm collection by the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in 2001–2019 and the cultivar ‘Gorizont’ in 1990–2019 in Yekaterinino Experiment Station of VIR (Tambov Province, Russia, 52°59′ N, 40°50′ E). A progressive shortening of the growing season and a yield increase were observed during the study both in the mean values for the tested accessions and in the cv. ‘Gorizont’. Grain yield variability of cv. ‘Gorizont’ across the years was also associated with 1000 grain weight variations. The models predict a further reduction in the growing season by 2.4 days/10 years, mainly caused by an increase in temperatures above 15 °C, and an increase in yield by 47.6 g/m2/10 years, mainly caused by an increase in the temperature in May. ANOVA demonstrated that the highest yields in Tambov Province were produced by accessions from Ulyanovsk Province, Ukraine, Moscow Province, Norway, Germany, and Poland.
Efficacy of Post-Emergence Herbicides against Important Weeds of Sugarcane in North-East Thailand
Authors: Aekrathok Phitsanu , Songsri Patcharin , Jongrungklang Nuntawoot , Gonkhamdee Santimaitree
Published: February 2021

Weeds represent a significant problem in Thailand’s sugarcane production. The various cycles of sugarcane cultivation result in degrees of weed severity in which each species requires a different weed control method, the most popular of which is the post-emergence herbicide method. However, sugarcane farmers often use incorrect rates, and operators’ safety is missing or applications are not selective, causing toxicity and interrupted growth of sugarcane. The effects of post-emergence herbicide types, time, and application rates are presented herein, through an experiment in Northeast Thailand, conducted in a randomized complete block design (RCBD) with four replications. The herbicides paraquat and ametryn were evaluated, with paraquat being more effective, yet resulting in higher toxicity and lower yield components. We may conclude through our experiments that ametryn and paraquat can be applied only once for sugarcane at the tillering stage and at rates of 540 and 2400 g ai ha−1, respectively. These rates were lower than the recommended rates, which represented the minimum doses necessary to control Thailand’s dominant weed species like Brachiaria distachya (L.) Stapf., Dactyloctenium aegyptium (L.) P.B., Praxelis clematidea R.M King & H. Rob and Pennisetum polystachion (L.) Schult. Consequently, the adequate control of weeds is a rather case-specific situation and therefore each weed species should be taken into account.
Radiographic Measurements of the Cardiac Silhouette and Comparison with Other Radiographic Landmarks in Wild Galahs (Eolophus roseicapilla)
Authors: Schnitzer Petra , Sawmy Shivananden , Crosta Lorenzo
Published: February 2021

Background: Part of the diagnostic workup for cardiac diseases is radiographic imaging. To determine an enlarged heart, species-specific reference values are necessary. Wild birds are rarely diagnosed with cardiac disease, and only a few studies have been done to investigate the cardiac silhouette in wild birds. Methods: In this retrospective study, the cardiac silhouette of 36 wild galahs, presented at the hospital, was investigated in relation to other anatomic landmarks like the thoracic width, clavicula width, synsacrum width, distance between the third and fourth rib, distance of the clavicula, and length and height of the sternum using a digital DICOM viewer. Results: The cardiac width was significant compared to the thoracic width with a minimum to maximum of 50 to 65%. The cardiac width compared with the coracoid width also showed significant results with a minimum to maximum range of 570 to 743%. A significant correlation was found between the weight and the cardiac width and length. Conclusion: The cardiac silhouette in wild galahs is easily measured in both radiographic views, and the heart size can be compared to other anatomical landmarks.
A Comparative Perspective on Three Primate Species’ Responses to a Pictorial Emotional Stroop Task
Authors: Hopper Lydia M. , Allritz Matthias , Egelkamp Crystal L. , Huskisson Sarah M. , Jacobson Sarah L. , Leinwand Jesse G. , Ross Stephen R.
Published: February 2021

The Stroop effect describes interference in cognitive processing due to competing cognitive demands. Presenting emotionally laden stimuli creates similar Stroop-like effects that result from participants’ attention being drawn to distractor stimuli. Here, we adapted the methods of a pictorial Stroop study for use with chimpanzees (N = 6), gorillas (N = 7), and Japanese macaques (N = 6). We tested all subjects via touchscreens following the same protocol. Ten of the 19 subjects passed pre-test training. Subjects who reached criterion were then tested on a standard color-interference Stroop test, which revealed differential accuracy in the primates’ responses across conditions. Next, to test for an emotional Stroop effect, we presented subjects with photographs that were either positively valenced (a preferred food) or negatively valenced (snakes). In the emotional Stroop task, as predicted, the primates were less accurate in trials which presented emotionally laden stimuli as compared to control trials, but there were differences in the apes’ and monkeys’ response patterns. Furthermore, for both Stroop tests, while we found that subjects’ accuracy rates were reduced by test stimuli, in contrast to previous research, we found no difference across trial types in the subjects’ response latencies across conditions.
Processed By-Products from Soy Beverage (Okara) as Sustainable Ingredients for Nile Tilapia (O. niloticus) Juveniles: Effects on Nutrient Utilization and Muscle Quality
Authors: Voss Glenise B. , Sousa Vera , Rema Paulo , Pintado Manuela. E. , Valente Luísa M. P.
Published: February 2021

The apparent digestibility coefficients (ADCs) of differently processed okara meals were assessed in Nile tilapia diets: dried okara not autoclaved (FOK), dried okara autoclaved (AOK), okara hydrolyzed with Alcalase (ALOK) or Cynara cardunculus proteases (CYOK), and hydrolyzed okara fermented with lactic bacteria: Lactobacillus rhamnosus R11 (CYR11OK) or Bifidobacterium animalis ssp. lactis Bb12 (CYB12OK). Okara processing significantly affected nutrient digestibility: dry matter ADC was highest in CYR11OK (80%) and lowest in FOK (40%). The lowest protein digestibility was observed in CYR11OK (72%), and the highest in AOK (97%) and CYOK (91%), evidencing the effectiveness of the autoclave and the use of C. cardunculus proteases to increase okara protein bioavailability. The inclusion of up to 20% of AOK or CYOK did not affect fish growth, nutrient utilization, or whole body composition of Nile tilapia. The flesh quality (color, pH, water activity, cohesiveness, elasticity and resilience) was not affected by the dietary incorporation of AOK or CYOK. Fish fed with AOK diets stand out for their high density of muscle fibers, particularly in AOK20, which can explain their high muscle firmness and may result in further hypertrophic growth. Altogether, results suggest that hydrolyzed or autoclaved okara are valuable ingredients for Nile tilapia diets.
Dog Tethering in Slovakia: Legal, Ethical and Behavioral Aspects and Dog Welfare Implications
Authors: Takáčová Daniela , Skurková Lenka , Mesarčová Lýdia , Lešková Lenka , Kottferová Lucia , Packová Anna , Vajányi Dávid , Kottferová Jana
Published: February 2021

Long-term tethering of dogs, or their keeping under unsuitable conditions can result in issues related to changes in their behavior as they may not satisfy their basic needs of life. These needs are discussed in this paper, along with cases when dogs unnecessarily have to endure cruelty and pain. The unavoidable tethering of a dog must not cause trauma and must be arranged in a way that it guarantees physical comfort. Failure to meet the basic needs of an animal may result in manifestation of fear and subsequent aggressiveness. Owners of animals are responsible for their life and health, and their obligations include eliminating the possibility of them hurting themselves or other beings. The relevant adopted legislative provisions should provide protection to animals and be enforceable, which currently appears rather difficult. Controlling and observation of the legislative provisions related to the tethering of dogs raises some difficulties for animal protection inspectors. It is necessary to focus on the specificities of keeping conditions of various dog breeds and on their individual features. Based on research and the relevant Slovak legislative provisions, this paper discusses various views on the practice of tethering dogs from the point of view of public safety and the ethical consequences of permanent dog tethering. Data on dog tethering in Slovakia were evaluated based on a survey and Slovak legal rules governing this issue were analyzed along with various views of public safety and the ethical consequences of permanent dog tethering.
Association between Experience of Pet Ownership and Psychological Health among Socially Isolated and Non-Isolated Older Adults
Authors: Ikeuchi Tomoko , Taniguchi Yu , Abe Takumi , Seino Satoshi , Shimada Chiho , Kitamura Akihiko , Shinkai Shoji
Published: February 2021

The psychological health effects of pet ownership have been widely studied, but only a few studies investigated its impact among socially isolated older adults. The present study aims to investigate the psychological health of older adults with or without the experience of pet (i.e., dog or cat) ownership who are socially isolated or not socially isolated. This study used cross-sectional data from 9856 community-dwelling older adults in a metropolitan area of Japan. Social and non-social isolation and type of pet ownership (i.e., dog or cat) were stratified to examine the psychological health. Logistic regression models indicated that, after adjusting for demographic and potential confounders, socially isolated older adults who never owned a dog were 1.22 times more likely to report lower psychological health in comparison to socially isolated current or past dog owners. No such difference was observed among cat owners. The results suggest that the experience of dog ownership may be effective to improve the psychological health among socially isolated older adult.
Development of Effective Antibacterial Treatment: Lessons from the Past and Novel Approaches
Authors: Di Luca Mariagrazia , Marzo Tiziano
Published: February 2021

In the last three decades, the appearance and rapid diffusion of antibiotic-resistant bacterial strains have been observed [...]