bluuebunny/crossref_metadata_2025 huggingface dataset:
Biphenotypic Sinonasal Sarcoma: Literature Review of a Peculiar Pathological Entity—The Neurosurgical Point of View
Authors: Corvino Sergio , Corazzelli Giuseppe , Mariniello Giuseppe , Iuliano Adriana , Altieri Roberto , Pontillo Giuseppe , Strianese Diego , Barbarisi Manlio , Elefante Andrea , de Divitiis Oreste
Published: November 2024

Background: Biphenotypic sinonasal sarcoma (BSNS) is a low-grade tumor of the sinonasal tract with frequent extension to the orbit and skull base. Due to its rare incidence and recent histopathological and molecular characterization, little data are available in regard to its natural history, treatment and surveillance protocol. Methods: A comprehensive literature review in Embase online electronic databases on BSNS was made. The analyzed factors included the patients’ sex and age, presenting symptoms and signs, anatomical origin and pattern of growth of the tumor, immunohistochemical and molecular features, time to treatment, type of treatment, surgical approach, extent of resection, peri- and post-operative complications, adjuvant therapies, clinical outcome, recurrence and overall survival rates. Results: This literature review involved 34 studies for an overall series of 149 cases of BSNS. The female (66.9%) and middle-aged populations (median 54.88 years old) were mainly affected. The most frequent clinical onset was nasal obstruction (81%), followed by facial discomfort (44%), epistaxis (15.5%) and ocular impairment (14.3%). Ethmoid sinus (67.8%) and nasal cavity (45.4%) were the most common anatomical site of tumor origin, while an extension to the orbit and skull base was registered in 28.7% and 24.5% of cases. Surgery was the main treatment, especially in the form of endoscopic endonasal approach (56.9%), and allowed for gross total resection in 79% of cases. The recurrence rate was 26.2%; three cases of tumor-related death were reported. Median follow-up was 4.6 years. Conclusions: Biphenotypic sinonasal sarcoma is a rare and unique tumoral entity in terms of biological and clinical behavior. Based on the current knowledge, surgery plays the leading role in treatment, accounting for gross total tumor resection in most cases, allowing for clinical symptom and sign resolution and presenting a low rate of perioperative complications. The type of approach and the aim of surgery should be assessed case by case according to patient and pathology features and the surgeon’s experience, as well as the aim of the treatment. Further studies including large surgical series and with long follow-up are required to define prognostic factors and guidelines of treatment for this peculiar pathological entity.
Ballooning and Bursting of Barrels and Pipes: A Rare Case of Suspected Vascular Ehlers–Danlos Disease
Authors: Agogbuo Ogechi , Kanuri Sri Harsha , Salinas Luis , Goweba Mohamed , Vahdat Khashayar , Chastian Oscar , Frase Larry
Published: November 2024

Vascular Ehler–Danlos disease (vEDS), a rare subtype of a rare disease, is a life-threatening disease, with an increased risk for spontaneous vascular or visceral rupture. These patients have fatal complications ranging from vascular aneurysms, dissection, and rupture of systemic vessels to frequent thromboembolic events, the common causes of death in these individuals with a shortened life span. In the present case, a 28-year-old male with history of shoulder dislocations and spontaneous colon perforation presented to the primary care clinic with right lower extremity swelling and pain. His history includes presentation to the emergency department with left lower leg swelling with compartment syndrome one year prior. A CT angiogram of lower extremities and abdomen revealed acute arterial extravasation of the left posterior tibial artery, indicating a ruptured aneurysm along with aneurysms of the splenic artery and left common iliac artery. He was treated with a saphenous vein graft, but was associated with post-operative complications that necessitated below-knee amputation. CT angiogram of his right leg revealed occlusion of the anterior tibial and peroneal arteries with aneurysms, and, ultimately, he was referred to a tertiary care center for aneurysm embolization. This case report emphasizes the frequent vascular complications encountered in vascular EDS patients, and thus advocates for close and regular monitoring for early referral and surgical management of their vascular anomalies. Finally, genetic counseling and screening of asymptomatic family members should be routinely implemented in these patients.
Peroxydisulfate Activation by Biochar from Banana Peel Promoted with Copper Phosphide for Bisphenol S Degradation in Aqueous Media
Authors: Ioannidi Alexandra A. , Logginou Orestia , Kouvelis Konstantinos , Petala Athanasia , Antonopoulou Maria , Mantzavinos Dionissios , Frontistis Zacharias
Published: November 2024

In this work, the decomposition of bisphenol S (BPS) by biochar derived from banana peel (BPB) promoted by copper phosphide (Cu3P) was examined. Different materials with Cu3P loadings from 0.25 to 4.00 wt.% on biochar were synthesized, characterized using the Brunauer–Emmett–Teller (BET) method, X-ray diffraction (XRD) and a scanning electron microscope (SEM) equipped with an energy dispersive spectrometer (EDS), and evaluated. Nearly all of the synthesized materials exhibited low to moderate adsorption capacity, attributable to their limited surface area (<3.1 m2/g). However, in the presence of sodium persulfate (SPS), the 2%Cu3P/ΒPB/SPS system was capable of removing 90% of 500 μg/L BPS in less than 10 min. The system’s performance was enhanced under inherent pH, and the reaction rate followed pseudo-first-order kinetics with respect to BPS and persulfate concentrations. Interestingly, the presence of 250 mg/L of sodium chloride had a negligible effect, while low to moderate inhibition was observed in the presence of bicarbonates and humic acid. In contrast, significant retardation was observed in experiments performed in real matrices, such as secondary effluent (WW) and bottled water (BW). According to scavenging experiments, both radical and non-radical mechanisms participated in the BPS degradation. Four transformation products were identified using the UHPLC/TOF-MS system in negative ionization mode, with two of them having higher molecular weights than BPS, while the other two TBPs involved the ring-opening reaction, and a BPS decomposition pathway was proposed.
Functional Nano-Metallic Coatings for Solar Cells: Their Theoretical Background and Modeling
Authors: Jacak Witold Aleksander
Published: November 2024

We have collected theoretical arguments supporting the functional role of nano-metallic coatings of solar cells, which enhance solar cell efficiency via by plasmon-strengthening the absorption of sun-light photons and reducing the binding energy of photoexcitons. The quantum character of the plasmonic effect related to the absorption of photons (called the optical plasmonic effect) is described in terms of the Fermi golden rule for the quantum transitions of semiconductor-band electrons induced by plasmons from a nano-metallic coating. The plasmonic effect related to the lowering of the exciton binding energy (called the electrical plasmonic effect) is of particular significance for metalized perovskite solar cells and is also characterized in quantum mechanics terms. The coupling between plasmons in nanoparticles from a coating with band electrons in a semiconductor substrate significantly modifies material properties (dielectric functions) both of the particles and the semiconductor, beyond the ability of the classical electrodynamics to describe.
Influence of Ionic Liquids on the Functionality of Optoelectronic Devices Employing CsPbBr3 Single Crystals
Authors: Alresheedi Faisal
Published: November 2024

Regulating the nucleation temperature and growth rates during inverse temperature crystallization (ITC) is vital for obtaining high-quality perovskite single crystals via this technique. Precise control over these parameters enables growing crystals optimized for various optoelectronic devices. In this study, it is demonstrated that incorporating a 1-butyl-3-methylimidazolium bromide (BMIB) ionic liquid into the precursor solution of cesium lead bromide (CsPbBr3) brings about a dual enhancement effect. This includes a reduction in nucleation temperature from 85 °C to 65 °C and a significant improvement in both optoelectronic characteristics and crystal properties. The CsPbBr3 single crystals grown using ITC with BMIB added (method (2)) demonstrate improved chemical and physical properties (crystallinity, lattice strain, nonradioactive recombination, and trap density) compared to CsPbBr3 single crystals produced through conventional 85 °C ITC alone (method (1)). The exceptional quality of CsPbBr3 single crystals produced with the inclusion of BMIB allowed for the development of a highly responsive optoelectronic device, demonstrating heightened sensitivity to green light. The findings of this investigation reveal that the growth of perovskite single crystals assisted by ionic liquid exerts a substantial impact on the characteristics of the crystals. This influence proves advantageous for the development of optoelectronic devices based on single crystals.
The Mosquitoes (Diptera: Culicidae) of Sonora: Distribution, Ecology, and the First Records of Aedes deserticola Zavortink and Toxorhynchites septentrionalis (Dyar and Knab) in México
Authors: Ortega-Morales Aldo I. , Quijano-Barraza Juan Manuel , Rodríguez-Pérez Mario A. , Hernández-Triana Luis M. , Wong-Corral Francisco , Correa-Morales Fabián
Published: November 2024

The diversity and distribution of mosquitoes from Sonora, Mexico, was documented through entomologic surveys conducted in the four physiographic regions and sub-regions of Sonora: the Sonoran Plain, the Sierra Madre Occidental, the Northern Mountains and Plains, and the Pacific Coastal Plain. Immature stages were collected from aquatic habitats, while adult mosquitoes were collected using Shannon traps, resting in vegetation, and by human landing collections. Overall, 11,316 specimens, which comprised 493 larvae, 224 larval exuviae, 400 pupal exuviae, 33 pupae, 4552 females, 5607 males, and seven male genitalia, were identified. Two subfamilies: Anophelinae and Culicinae, seven tribes, 10 genera, 23 subgenera, and 56 species are reported below. Of these, one tribe, one genus, five subgenera, and 15 species were recorded for the first time in Sonora. Two species, Aedes deserticola Zavortink and Toxorhynchites septentrionalis (Dyar and Knab), are the first recordings of their kind nationwide. Toxorhynchites septentrionalis was also barcoded with the cytochrome oxidase subunit 1 (COI) gene. The presence of Psorophora columbiae (Dyar and Knab) is confirmed in Mexico. Taxonomic notes, new geographic distribution limits of mosquitoes in Sonora, and information regarding their importance as disease vectors are provided. By adding Ae. deserticola and Tx. septentrionalis and confirming Ps. columbiae in Mexico, there are currently 251 mosquito species in the Country.
Intelligent Inversion Analysis of Surrounding Rock Parameters and Deformation Characteristics of a Water Diversion Surge Shaft
Authors: Zou Xing-Wei , Zhou Tao , Li Gan , Hu Yu , Deng Bo , Yang Tao
Published: November 2024

The water diversion surge shaft is vital for a hydropower station. However, the complex geological properties of the surrounding rock make it challenging to obtain its mechanical parameters. A method combining particle swarm optimization (PSO) and support vector machine (SVM) algorithms is proposed for estimating these parameters. According to the engineering geological background and support scheme, a three-dimensional model of the water diversion surge shaft is established by FLAC3D. An orthogonal test is designed to verify the accuracy of the numerical model. Then, the surrounding rock mechanical parameter database is established. The PSO-SVM intelligent inversion algorithm is used to invert the optimal values of the mechanical parameters of the surrounding rock. The support for excavating the next layer depends on the mechanical parameters of the current rock layer. An optimized design scheme is then compared and analyzed with the original support scheme by considering deformation and plastic characteristics. The research results demonstrate that the PSO-SVM intelligent inversion algorithm can effectively improve the accuracy and efficiency of the inversion of rock mechanical parameters. Under the influence of excavation, the surrounding rock in the plastic zone mainly fails in shear, with maximum deformation occurring in the middle and lower parts of the excavation area. The maximum deformation of the surrounding rock under support with long anchor cables is 0.6 cm less than that of support without long anchor cables and 4.07 cm less than that of support without an anchor. In the direction of the maximum and minimum principal stress, the maximum depth of the plastic zone under the support with long anchor cables is 1.3 m to 2.6 m less than that of the support without long anchor cables and the support without an anchor. Compared with the support without long anchor cables and support without an anchor, the support with long anchor cables can effectively control the deformation of the surrounding rock and limit the development of the plastic zone.
HIV Self-Testing: A Discussion on the Benefits, Limitations, and Implications for Public Health with a Focus on Poland
Authors: Kozieł Aleksandra , Domański Igor , Szymczak Aleksandra , Dudzik Tomasz , Knysz Brygida , Szetela Bartosz
Published: November 2024

Background/Objectives: A late HIV diagnosis represents a significant public health concern in Poland, with approximately 50% of patients being identified as late presenters (LPs), resulting in the delayed initiation of treatment. This study assesses the potential of HIV self-testing (HIVST) to enhance early detection, particularly among heterosexual individuals, and evaluates its advantages and limitations within the Polish context. Methods: This study examines the advantages and disadvantages of HIVST by analyzing data from various studies. It focuses on the acceptability, sensitivity, and specificity of HIVST, comparing blood-based and oral fluid-based tests. Moreover, the economic impact and potential public health benefits of HIVST in Poland are evaluated. Results: HIVST is well-accepted, especially among key populations; it can reduce stigma and enhance privacy. The results of studies conducted in Poland indicate that heterosexuals are more inclined to use self-testing methods than traditional diagnostic procedures. On the other hand, HIVST has the potential for false-negative results due to the serological window and the possibility of missed diagnoses of other sexually transmitted infections (STIs). Moreover, the cost of HIVST remains a significant barrier, as it is not publicly funded in Poland. Conclusions: Despite its limitations, HIVST offers a number of significant benefits, including increased rates of testing and earlier detection, which could prove vital in reducing the transmission of HIV in Poland. This study proposes that increased funding, the integration of HIVST into public health strategies, and further research to enhance its implementation, alongside education and support for its effective use, should be prioritized.
Impact of Precision in Staging Acute Kidney Injury and Chronic Kidney Disease on Treatment Outcomes: An Observational Study
Authors: Endrich Olga , Nakas Christos T. , Triep Karen , Fiedler Georg M. , Caro Jaime J. , McGuire Alistair
Published: November 2024

(1) Background: “Kidney Disease: Improving Global Outcomes” (KDIGO) provides guidelines for identifying the stages of acute kidney injury (AKI) and chronic kidney disease (CKD). A data-driven rule-based engine was developed to determine KDIGO staging compared to KD-related keywords in discharge letters. (2) Methods: To assess potential differences in outcomes, we compare the patient subgroups with exact KDIGO staging to imprecise or missing staging for all-cause mortality, in-hospital mortality, selection bias and costs by applying Kaplan–Meier analysis and the Cox proportional hazards regression model. We analysed 63,105 in-patient cases from 2016 to 2023 at a tertiary hospital with AKI, CKD and acute-on-chronic KD. (3) Results: Imprecise and missing CKD staging were associated with an 85% higher risk of all-cause and in-hospital mortality (CI: 1.7 to 2.0 and 1.66 to 2.03, respectively) compared to exact staging for any given disease status; imprecise or missing AKI staging increased in-hospital mortality risk by 56% and 57% (CI: 1.43 to 1.70 and 1.37 to 1.81, respectively) in patients with AKI. (4) Conclusions: Exact staging is associated with better outcomes in KD management. Our study provides valuable insight into potential quality and outcome improvements and lower costs, considering elderly patients, women and patients with acute-on-chronic KD as the most vulnerable.
A Design of NLOS Communication Scheme Based on SC-FDE with Cyclic Suffix for UAV Payload Communication
Authors: Wang Peng , Xiang Xin , Wang Rui , Dong Pengyu , Li Qiao
Published: November 2024

Non-line-of-sight (NLOS) communication with severe loss always leads to performance degradation in unmanned aerial vehicle (UAV) payload communication. In this paper, a UAV NLOS communication scheme based on single-carrier frequency domain equalization with cyclic prefix and cyclic suffix (CP/CS-SC-FDE) is designed. First, the reasons behind the generation of later intersymbol interference (LISI) in UAV NLOS communication are investigated. Then, the frame structure of conventional single-carrier frequency domain equalization with cyclic prefix (CP-SC-FDE) is improved, and the UAV NLOS communication frame structure based on cyclic prefix (CP) and cyclic suffix (CS) is designed. Furthermore, a channel estimation algorithm applicable to this scheme is proposed. The numerical results show that this UAV communication scheme can eliminate intersymbol interference (ISI) in NLOS communication. Compared with the conventional CP-SC-FDE system, this scheme can achieve excellent performance in the Rayleigh channel and other standard NLOS channels. In the CP/CS-SC-FDE system, the BER result is similar to that under ideal synchronization.
Infrared and Visible Camera Integration for Detection and Tracking of Small UAVs: Systematic Evaluation
Authors: Pereira Ana , Warwick Stephen , Moutinho Alexandra , Suleman Afzal
Published: November 2024

Given the recent proliferation of Unmanned Aerial Systems (UASs) and the consequent importance of counter-UASs, this project aims to perform the detection and tracking of small non-cooperative UASs using Electro-optical (EO) and Infrared (IR) sensors. Two data integration techniques, at the decision and pixel levels, are compared with the use of each sensor independently to evaluate the system robustness in different operational conditions. The data are submitted to a YOLOv7 detector merged with a ByteTrack tracker. For training and validation, additional efforts are made towards creating datasets of spatially and temporally aligned EO and IR annotated Unmanned Aerial Vehicle (UAV) frames and videos. These consist of the acquisition of real data captured from a workstation on the ground, followed by image calibration, image alignment, the application of bias-removal techniques, and data augmentation methods to artificially create images. The performance of the detector across datasets shows an average precision of 88.4%, recall of 85.4%, and mAP@0.5 of 88.5%. Tests conducted on the decision-level fusion architecture demonstrate notable gains in recall and precision, although at the expense of lower frame rates. Precision, recall, and frame rate are not improved by the pixel-level fusion design.
Robot Localization Method Based on Multi-Sensor Fusion in Low-Light Environment
Authors: Wang Mengqi , Lian Zengzeng , Núñez-Andrés María Amparo , Wang Penghui , Tian Yalin , Yue Zhe , Gu Lingxiao
Published: November 2024

When robots perform localization in indoor low-light environments, factors such as weak and uneven lighting can degrade image quality. This degradation results in a reduced number of feature extractions by the visual odometry front end and may even cause tracking loss, thereby impacting the algorithm’s positioning accuracy. To enhance the localization accuracy of mobile robots in indoor low-light environments, this paper proposes a visual inertial odometry method (L-MSCKF) based on the multi-state constraint Kalman filter. Addressing the challenges of low-light conditions, we integrated Inertial Measurement Unit (IMU) data with stereo vision odometry. The algorithm includes an image enhancement module and a gyroscope zero-bias correction mechanism to facilitate feature matching in stereo vision odometry. We conducted tests on the EuRoC dataset and compared our method with other similar algorithms, thereby validating the effectiveness and accuracy of L-MSCKF.
Machine Learning and Deep Learning Applications in Disinformation Detection: A Bibliometric Assessment
Authors: Sandu Andra , Cotfas Liviu-Adrian , Delcea Camelia , Ioanăș Corina , Florescu Margareta-Stela , Orzan Mihai
Published: November 2024

Fake news is one of the biggest challenging issues in today’s technological world and has a huge impact on the population’s decision-making and way of thinking. Disinformation can be classified as a subdivision of fake news, the main purpose of which is to manipulate and generate confusion among people in order to influence their opinion and obtain certain advantages in multiple domains (politics, economics, etc.). Propaganda, rumors, and conspiracy theories are just a few examples of common disinformation. Therefore, there is an urgent need to understand this phenomenon and offer the scientific community a paper that provides a comprehensive examination of the existing literature, lay the foundation for future research areas, and contribute to the fight against disinformation. The present manuscript provides a detailed bibliometric analysis of the articles oriented towards disinformation detection, involving high-performance machine learning and deep learning algorithms. The dataset has been collected from the popular Web of Science database, through the use of specific keywords such as “disinformation”, “machine learning”, or “deep learning”, followed by a manual check of the papers included in the dataset. The documents were examined using the popular R tool, Biblioshiny 4.2.0; the bibliometric analysis included multiple perspectives and various facets: dataset overview, sources, authors, papers, n-gram analysis, and mixed analysis. The results highlight an increased interest from the scientific community on disinformation topics in the context of machine learning and deep learning, supported by an annual growth rate of 96.1%. The insights gained from the research bring to light surprising details, while the study provides a solid basis for both future research in this area, as well for the development of new strategies addressing this complex issue of disinformation and ensuring a trustworthy and safe online environment.
Likely Response to a Hypothetical Menthol Cigarette Ban Among Adults with Mood Disorders Who Smoke Menthol Cigarettes and Have No Current Plans to Quit Smoking
Authors: Mazhar Laraib , Foulds Jonathan , Allen Sophia I. , Veldheer Susan , Hrabovsky Shari , Yingst Jessica M.
Published: November 2024

Background: There is limited evidence on how the United States Food and Drug Administration’s (FDA) proposed ban on menthol cigarettes and flavored cigars will impact individuals with mood disorders who smoke menthol cigarettes. This study aimed to evaluate how individuals with mood disorders who smoke menthol cigarettes might respond to a hypothetical ban on menthol cigarettes, explore the reasons for their current use, and examine how these reasons are associated with participants’ characteristics. Methods: Study data were collected at baseline from adults (18+ years) with mood disorders who participated in a randomized controlled trial evaluating the impact of gradual nicotine reduction. Participants were individuals who smoked and had no plan to quit in the next six months. They reported demographics and tobacco consumption patterns, interest in quitting, and responded to a hypothetical question on a potential ban on menthol cigarettes. The question asked participants which actions they would most likely take if menthol-flavored cigarettes were banned. Means and frequencies were used to describe the sample. Logistic regression was used to determine factors associated with each reason for menthol use (less harmful, better flavor, less harsh on the throat, and less harsh on the chest). Results: Participants (n = 77) were an average age of 42.5 (SD 12.5) years, 61% (n = 47) were female, 68.8% (n = 53) identified as White, and 5.2% (n = 4) identified as Hispanic. On average, participants reported currently smoking 18.1 (SD 9.9) cigarettes per day and had smoked for 23.9 (SD 13.6) years. About 58.4% of participants (n = 45) expressed their intention to switch to non-menthol cigarettes, 19.5% (n = 15) intended to transition to a different type of tobacco product, and 22.1% (n = 17) intended to quit smoking entirely without substitution. The most endorsed reason for using menthol cigarettes was better flavor (89.6%, n = 69/77), followed by less harshness on the throat (41.3%, n = 31/75) and chest (40%, n = 30/75), and the belief that they were less harmful than non-menthol cigarettes (24%, n = 18/75). Older age was associated with the belief that menthol cigarettes were less harmful (OR = 1.06; p = 0.02). Conclusion: Among individuals with mood disorders and who smoke menthol cigarettes and have no plans to quit smoking, 22.1% may try to quit smoking if a menthol ban is implemented, while the majority (58.4%) stated that they would switch to non-menthol cigarettes. As the reasons for using menthol cigarettes included perceived lower harm, there is a need for targeted public awareness campaigns to correct misconceptions about the harms of menthol cigarettes.
Identification and Characterization of EIN3/EIL Transcription Factor Family Members in Pinus massoniana Lamb.
Authors: Yu Wenya , Ren Xingyue , Zhang Jingjing , Huang Zichen , Zhao Yulu , Zhang Mengyang , Yao Sheng , Ji Kongshu
Published: November 2024

Transcription factors refer to types of proteins that perform significant functions in the process of gene expression regulation. The ethylene insensitive 3/ethylene insensitive 3-like (EIN3/EIL) family, functioning as significant transcription factors regulating ethylene, plays a critical role in the growth and development of plants and participates in the plant’s response to diverse environmental stresses. Pinus massoniana is an excellent native tree with high economic and ecological value. However, the study of EIN3/EIL genes in gymnosperms, for instance, P. massoniana, is still relatively limited. In this research, four putative EIN3/EIL genes were identified in the transcriptome of P. massoniana. Bioinformatics analysis showed that PmEIL genes contain a highly conserved EIN3 domain and other structural features of acidic, proline-rich and glutamine-rich sites. The molecular evolution tree analysis demonstrated that the EIN3/EIL family was partitioned into three categories (A, B, and C), and the number, type, and distribution of conserved motifs grouped in one category were similar. The results of qRT-PCR indicated that the expression levels of PmEIL genes were markedly elevated in needles compared to other tissues. Through the analysis of expression patterns of the PmEIL genes under various stress treatments, it was found that the PmEIL genes could participate in plant hormone stimulation induction, osmosis, drought and other response processes. In addition, PmEIL is a nuclear localization protein. PmEIL1, PmEIL3, and PmEIL4 are transcriptional activators, while PmEIL2 is a transcriptional suppressor. This research provides a basis for further elucidating the function of EIN3/EIL transcription factors in growth, development and stress response of P. massoniana.
Genome-Wide Analysis of Heat Shock Protein Family and Identification of Their Functions in Rice Quality and Yield
Authors: Wang Hong , Charagh Sidra , Dong Nannan , Lu Feifei , Wang Yixin , Cao Ruijie , Ma Liuyang , Wang Shiwen , Jiao Guiai , Xie Lihong , Shao Gaoneng , Sheng Zhonghua , Hu Shikai , Zhao Fengli , Tang Shaoqing , Chen Long , Hu Peisong , Wei Xiangjin
Published: November 2024

Heat shock proteins (Hsps), acting as molecular chaperones, play a pivotal role in plant responses to environmental stress. In this study, we found a total of 192 genes encoding Hsps, which are distributed across all 12 chromosomes, with higher concentrations on chromosomes 1, 2, 3, and 5. These Hsps can be divided into six subfamilies (sHsp, Hsp40, Hsp60, Hsp70, Hsp90, and Hsp100) based on molecular weight and homology. Expression pattern data indicated that these Hsp genes can be categorized into three groups: generally high expression in almost all tissues, high tissue-specific expression, and low expression in all tissues. Further analysis of 15 representative genes found that the expression of 14 Hsp genes was upregulated by high temperatures. Subcellular localization analysis revealed seven proteins localized to the endoplasmic reticulum, while others localized to the mitochondria, chloroplasts, and nucleus. We successfully obtained the knockout mutants of above 15 Hsps by the CRISPR/Cas9 gene editing system. Under natural high-temperature conditions, the mutants of eight Hsps showed reduced yield mainly due to the seed setting rate or grain weight. Moreover, the rice quality of most of these mutants also changed, including increased grain chalkiness, decreased amylose content, and elevated total protein content, and the expressions of starch metabolism-related genes in the endosperm of these mutants were disturbed compared to the wild type under natural high-temperature conditions. In conclusion, our study provided new insights into the HSP gene family and found that it plays an important role in the formation of rice quality and yield.
Dietary Influence on Growth, Physicochemical Stability, and Antimicrobial Mechanisms of Antimicrobial Peptides in Black Soldier Fly Larvae
Authors: Liu Shaojuan , Raheel Tariq Muhammad , Zhang Qihui , Wang Hui , Wang Fei , Zheng Chaozhong , Li Kuntai , Zhuang Zhikai , Wang Leiyu
Published: November 2024

Safe antibiotic substitutes are needed given the rise in antimicrobial resistance, environmental contamination, and stringent antibiotic regulations. Insect-derived antimicrobial peptides (AMPs) are promising candidates due to their antimicrobial activity, stability, and safety. This study investigates the antimicrobial mechanism of crude AMP extracts and their physicochemical characteristics in black soldier fly larvae (BSFL). The results indicated that BSFL reared on a wheat bran diet exhibited significantly improved growth performance and AMP production when compared to the other three diets. AMP extracts showed enhanced antimicrobial activity and physicochemical stability, including temperatures and metal ions except Cu+. Moreover, AMP extracts disrupted the cell membrane and inhibited the cell cycle of Staphylococcus aureus (S. aureus), thus exhibiting antimicrobial activity. Furthermore, transcriptomic and KEGG enrichment analyses identified 509 differentially expressed genes (DEGs) related to the Toll and IMD signaling pathways. STRING and GeneMANIA analyses confirmed the association of these pathways with immune response and AMP secretion. qRT-PCR results showed elevated expression of immune genes (GNBP3, NFKBIA, GADD45, and Spz) in BSFL following S. aureus immunization, consistent with RNA-seq findings. These findings offer a valuable reference for using AMPs as antibiotic substitutes in animal feeds and highlight the need for further research on AMP purification and the synergistic regulation of protein synthesis and AMP production in BSFL.
Preoperative Narcotic Education in Spine Surgery: A Retrospective Study
Authors: Abbas Anas M. , Ngan Alex , Li Jian H. , Abbas Araf M. , Pandya Aadi , Ahmad Salman , Jung Bongseok , Shahsavarani Shaya , Verma Rohit B.
Published: November 2024

Background/Objectives: The objective of this study was to determine whether preoperative opioid education reduces opioid consumption after spine surgery and which educational methods are the most effective. Orthopedists are the most likely to prescribe opioids among all specialists. To alleviate the prescription opioid crisis, studies have identified ways to taper narcotic dosage and use following surgery. The role of preoperative education and its varying modalities on opioid consumption following spine surgery has yet to be reported in the literature. Methods: The study group received formal education describing the use of opioids, side effects, and alternatives to pain management. Patients were to choose their preferred modality of a 2 min narrated video and two handouts to be watched and read in their individual time, attending a small class led by a physician assistant where they watched the 2 min narrated video along with reading the two handouts or receiving a one-on-one session with the treating spine surgeon. Meanwhile, the control group received standard preoperative education. Refill prescriptions were collected from patients’ electronic medical record charts at the 2-week, 1-month, 3-month, and 6-month postoperative follow-ups. The primary outcome measure was morphine equivalents (MME) of prescription opioids at six months following spine surgery. Results: At 2 weeks postoperatively, there were no statistically significant differences between patients who received any formal narcotic education and those who did not. At 1 and 3 months postoperatively, the video education group (p-value < 0.001), class education group (p-value < 0.001), and the one-on-one education group (p-value < 0.05) all had significant reductions in opioid consumption. At 6 months postoperatively, only the video education group (p-value < 0.001) and the class education group (p-value < 0.01) had significant reductions in opioid consumption. Conclusions: A two-fold approach with a video and handouts significantly decreases the prescription dosage at six months postoperatively and allows for early opioid cessation after undergoing spine surgery.
Risk Factors, Comorbidities, and Prevention of Cardiovascular Diseases: Don’t Forget the Primary Cause!
Authors: Roubille François
Published: November 2024

Cardiovascular diseases (CVDs) remain a leading cause of mortality worldwide. Despiteinnovative treatments, both pharmacological and interventional, CVDs continue toprogress. This is largely due to the increased incidence of diseases such as diabetes andother well-established cardiovascular risk factors, as well as cumulative comorbidities andemerging risk factors.
Renal Denervation in Heart Failure Treatment: Data for a Self-Fulfilling Prophecy
Authors: Dimitriadis Kyriakos , Iliakis Panagiotis , Pyrpyris Nikolaos , Tatakis Fotis , Fragkoulis Christos , Mantziaris Vasileios , Plaitis Aristides , Beneki Eirini , Tsioufis Panagiotis , Hering Dagmara , Kollias Anastasios , Konstantinidis Dimitrios , Tsioufis Konstantinos
Published: November 2024

Renal denervation (RDN), a transcatheter renal sympathetic nerve ablation procedure, is a relatively novel established procedure for the treatment of hypertension, with it being recognized as a third option for hypertension management in the most recent European guidelines, together with pharmacotherapy, for achieving blood pressure targets. Given the relationship between both hypertension and sympathetic overdrive and the development of heart failure (HF), even studies at the dawn of research on RDN explored it as a treatment to overcome diuretic resistance in those patients. As it is now recognized that RDN does not only have organ-specific but also systemic effects, several investigators have aimed to delineate whether renal sympathetic denervation could alter the prognosis, symptoms, and adverse events of HF patients. Data are available in both HF patients with reduced and preserved ejection fraction. As the significance of neuromodulation is gaining grounds in the HF therapeutic arsenal, in this review, we aim to provide a rationale for using RDN in HF and an up-to-date overview of available data in both HF phenotypes, as well as discuss the future of neuromodulatory therapy in HF management.
The Management of Interstitial Lung Disease in the ICU: A Comprehensive Review
Authors: Dhanani Zehra , Gupta Rohit
Published: November 2024

Interstitial lung disease (ILD) encompasses a diverse group of parenchymal lung diseases characterized by varying degrees of inflammation and/or fibrosis. Patients with ILD frequently require hospitalization, with many needing intensive care unit (ICU) admission, most often due to respiratory failure. The diagnosis and management of ILD in the ICU present unique challenges. Diagnosis primarily relies on chest CT imaging to identify fibrosis and inflammation. Acute exacerbations, whether in idiopathic pulmonary fibrosis (IPF) or non-IPF ILD, require careful evaluation of potential triggers and differential diagnoses. Bronchoalveolar lavage may provide valuable information, such as the identification of infections, but carries risks of complications. Biopsies, whether transbronchial or surgical, can also be informative but pose significant procedural risks. Corticosteroids are the cornerstone of treatment for acute exacerbations of IPF, with higher doses potentially benefiting non-IPF ILD. Additional immunosuppressive agents may be used in cases with evidence of inflammation. Oxygen supplementation, particularly with high-flow nasal cannula, is often employed to manage severe hypoxemia, while noninvasive ventilation can be useful for worsening hypoxemia and/or hypercapnia. When mechanical ventilation is used, it is recommended to target low tidal volumes to minimize lung injury; high PEEP may be less effective and even associated with increased mortality. Prone positioning can improve oxygenation in severely hypoxemic patients. In addition to ventilatory strategies, careful fluid management and addressing concomitant pulmonary hypertension are essential components of care. Extracorporeal membrane oxygenation is a high-risk intervention reserved for the most severe cases. Lung transplantation may be considered for end-stage ILD patients in the ICU, with outcomes dependent on the urgency of transplantation and the patient’s overall condition. Managing ILD in the ICU requires a multidisciplinary approach, and despite recent advances, mortality remains high, emphasizing the need for continued research and individualized treatment strategies.
Comparative Efficacy of Different Pharmacological Treatments for Pityriasis Rosea: A Network Meta-Analysis
Authors: Ciccarese Giulia , Facciorusso Antonio , Herzum Astrid , Fidanzi Cristian , Recalcati Sebastiano , Foti Caterina , Drago Francesco
Published: November 2024

Background/Objectives: Pityriasis rosea (PR) is a self-limiting exanthematous disease associated with the endogenous reactivation of human herpesviruses (HHV)-6 and HHV-7. Classically, the lesions gradually resolve, leaving no sequelae. Therefore, the best treatment is reassuring the patient and suggesting a resting period. However, atypical PR cases characterized by extensive, persistent lesions and systemic symptoms may impact the patient’s quality of life, and, therefore, a treatment can be prescribed. There is limited evidence on the comparative effectiveness of pharmacological treatments for PR; therefore, we performed a network meta-analysis to compare these interventions. Methods: Overall, 12 randomized control trials (RCTs) were identified. The outcomes were itch resolution and rash improvement. Results were expressed as risk ratio (RR) and 95% confidence interval (CI). We also calculated the relative ranking of the interventions for achieving the aforementioned outcomes as their surface under the cumulative ranking (SUCRA). Results: On network meta-analysis, only oral steroids and the combination of oral steroids+antihistamine resulted significantly superior to the placebo in terms of itch resolution (RR 0.44, CI 0.27–0.72 and RR 0.47, CI 0.22–0.99). Oral steroids resulted in the best treatment (SUCRA 0.90) for itch resolution. In terms of rash improvement, only acyclovir and erythromycin resulted significantly superior to placebo (RR 2.55, CI 1.81–3.58; and RR 1.69, CI 1.23–2.33), and acyclovir outperformed all the other tested interventions. Consequently, acyclovir ranked as the best intervention (SUCRA score 0.92). Conclusions: Acyclovir represents the best option for patients with PR that have extensive, persistent lesions or systemic symptoms. Steroids and antihistamines seemed the best treatment for itch resolution.
Clinical, Radiological, and Surgical Risk Factors for Endoscopic Anastomotic Recurrence Following Surgery in Crohn’s Disease
Authors: Minordi Laura Maria , Sacchetti Franco , Balzano Domenico , Maresca Rossella , D’Angelo Francesca Bice , Larosa Luigi , Carano Davide , Laterza Lucrezia , Pugliese Daniela , Caprino Paola , Potenza Angelo Eugenio , Scaldaferri Franco , Sofo Luigi , Sala Evis
Published: November 2024

Objective: This study investigated the radiological, clinical, and surgical factors linked to the risk of endoscopic recurrence following ileocolic resection for Crohn’s disease. Materials and Methods: We conducted a retrospective analysis of data from all patients who underwent primary ileocecal resection for Crohn’s disease in a single colorectal unit between 2004 and 2020. We analyzed the potential risk factors subdivided by the clinical, radiological, and surgical factors associated with morphological recurrence, as detected by endoscopy within 2 years after surgery. Cox regression was employed to ascertain the risk factors associated with such recurrence. Results: In total, 63 patients were included, and 24 (38%) had endoscopic recurrence. The age of the patient at the time of surgery was identified as a significant clinical factor associated with the risk of recurrence (HR: 1.04; p = 0.003), indicating that the probability of recurrence increases by 1% as the surgical age increases each year. The radiological factors associated with an increased risk of recurrence included localization in the distal ileum (HR: 3.526; p = 0.015), the number of pathological small-bowel segments affected by the disease (HR: 1.15; p = 0.004), and the total length of the pathological intestinal segment (HR: 1.002; p = 0.014). The presence of granulomas (HR: 6.003; p = 0.004) and the length of the resected bowel (HR: 1.01; p = 0.003) were surgical factors associated with a higher risk of recurrence. Conclusions: This study delineated several clinical, radiological, and surgical factors that serve as predictors for the endoscopic recurrence of Crohn’s disease after surgery.
ECC at LLETZ—An Exploratory Retrospective Cohort Study
Authors: Paternostro Chiara , Joura Elmar A. , Ott Johannes , Ghobrial Stefan , Langthaler Eva M. , Pils Sophie
Published: November 2024

Background: The aim of this study is to evaluate the distribution of the histological results of the endocervical curettage (ECC) at large loop excision of the transformation zone (LLETZ) as well as the additional diagnostic information and its impact on further clinical treatment recommendations in accordance with national guidelines. The ECC in addition to LLETZ can serve to detect (pre)cancerogenic lesions above the endocervical cone margin, although its predictive value as well as diagnostic accuracy remains vague due to limited studies performed on this topic. Methods: In this retrospective cohort study, 1121 patients who underwent LLETZ and concomitant ECC during a twelve-year period (2009–2021) were analyzed. The main outcome parameters were the histological diagnosis and incidence of adequate or inadequate ECC specimens. Results: In 1.7% of the specimens, ECC performed at the completion of LLETZ yielded additional diagnostic information. The histological result of the ECC had an impact on subsequent therapeutic approach in 2 of the 1121 patients (0.2%). Furthermore, a negative ECC yielded a low negative predictive value (NPV) of 43.8% for the detection of residual disease. Conclusions: As current guidelines increasingly support the performance of an HPV test six months after LLETZ as an indicator of treatment success irrespective of the margin status, the routine performance of an ECC at LLETZ remains questionable.
Optimizing Bone Regeneration with Demineralized Dentin-Derived Graft Material: Impact of Demineralization Duration in a Rabbit Calvaria Model
Authors: Lee Bounghoon , Choi Hyunsuk , Sohn Dong-Seok
Published: November 2024

This study evaluated the regenerative potential of demineralized dentin-derived matrix (DDM) as a bone graft material in rabbit calvaria. DDM, sourced from extracted teeth, is emerging as an alternative to traditional grafts like allografts and xenografts. We aimed to identify the most effective demineralization protocol to optimize the regenerative capacity of DDM. Four groups were compared: a control group without grafts, a non-demineralized DDM group, and two demineralized DDM groups (15 and 30 min demineralization). Histomorphometric analysis was conducted in a randomized and blinded setting at 2, 4, and 8 weeks post-graft placement. The results revealed that the 15 min demineralized DDM group showed the most significant new bone formation (42.51% ± 6.40% at 8 weeks; p < 0.05), suggesting its potential as a highly effective regenerative graft material.
Influence of Interpass Temperature on the Simulated Coarse-Grained Heat-Affected Zone of a Circumferentially Welded 2.25Cr-1Mo Steel Pipe Joint
Authors: Dornelas Paulo Henrique Grossi , Payão Filho João da Cruz , Moraes e Oliveira Victor Hugo Pereira , Farias Francisco Werley Cipriano
Published: November 2024

To reduce manufacturing costs, energy companies aim to maximize the deposition rate during welding operations by increasing the interpass temperature (IT), thereby minimizing the cooling time. However, IT can significantly affect weldment performance, particularly its Charpy V-notch (CVN) impact energy (toughness). The present study investigates the effect of increasing IT beyond the limit specified by the ASME B31.3 (315 °C) on the CVN impact energy (−30 °C) of the simulated coarse-grained heat-affected zone (CGHAZ) of a 2.25Cr-1Mo steel submerged arc welded (SAW). The CGHAZ thermal cycles were obtained through finite element method simulations and physically replicated using a Gleeble machine. The increase in IT beyond the ASME-specified limit significantly reduces the CVN impact energy of the CGHAZ. However, the values obtained remained above the minimum required threshold (NORSOK M630, 42 J). The main effect of increased IT was grain coarsening. Additionally, an inverse linear relationship was observed between effective grain size (EGS) and CVN impact energy. The steel’s microstructure showed non-significant sensitivity to variations in IT within the studied range. These findings suggest that, under the conditions studied, increasing IT could be a viable option for optimizing production by reducing welding time and potentially lowering costs.