bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Identification of Two Rare Fusions, PDK1-ALK and STRN-ALK, That Coexist in a Lung Adenocarcinoma Patient and the Response to Alectinib
Authors: Zeng Hao , Li Yalun , Wang Ye , Huang Meijuan , Zhang Yan , Tian Panwen , Li Weimin
Published: August 2021

Several double ALK fusions coexisting in one patient have been reported. However, few studies have reported the clinical efficacy of ALK inhibitors in rare double ALK fusions. Here, we described a rare PDK1-ALK, STRN-ALK double-fusion variant in a patient with metastatic lung adenocarcinoma. The patient responded well to alectinib (600 mg) twice daily. This case shows a promising treatment option for patients with rare ALK double-fusion variants.
The Efficacy and Safety of Continuous Intravenous Endostar Treatment Combined With Concurrent Chemoradiotherapy in Patients With Locally Advanced Cervical Squamous Cell Carcinoma: A Randomized Controlled Trial
Authors: Shu Hang , Dong Yaqin , Xu Zhonghua , Luo Weiwei , Xu Lei , Zhu Haochen , Cheng Linghui , Lv Yin
Published: August 2021

Objective

To investigate the short-term efficacy and safety of Endostar combined with concurrent chemoradiotherapy in the treatment of locally advanced cervical squamous cell carcinoma (LACSC).

Methods

A total of 91 patients with LACSC admitted to the First Affiliated Hospital of Anhui Medical University from June 2019 to December 2020 were randomly assigned to either the experimental group (n = 48) or control group (n = 43). The control group received radiotherapy for cervical cancer and paclitaxel combined with platinum chemotherapy (CCRT), and the experimental group received Endostar continuous intravenous infusion of anti-angiogenic therapy plus CCRT. The short-term efficacy, common clinical indicators, tumor indicators, changes in serum vascular endothelial growth factor-A (VEGF-A), and the occurrence of adverse events (AEs) were explored after treatment.

Results

Compared with the control group, the complete response (CR) rate in the experimental group was significantly increased (83.33% vs 65.12%, P < 0.05). Both routine indicators and tumor indicators in the two groups were significantly decreased compared to before treatment. Compared with the control group, patients in the experimental group had higher incidences of neutropenia, hypertension, and infection, but lower incidence of nausea. After treatment, the serological expression of VEGF-A was significantly decreased in both groups.

Conclusion

Endostar combined with CCRT in the treatment of LACSC can further improve the efficacy of CR rate and significantly reduce serum tumor indicators and VEGF-A levels, with mild and controllable AEs. Endostar combined with CCRT is expected to be a new treatment regimen for LACSC.
Type 2 Diabetes Mellitus Intersects With Pancreatic Cancer Diagnosis and Development
Authors: Duan Xiaoye , Wang Weihao , Pan Qi , Guo Lixin
Published: August 2021

The relationship between type 2 diabetes mellitus (T2DM) and pancreatic cancer (PC) is complex. Diabetes is a known risk factor for PC, and new-onset diabetes (NOD) could be an early manifestation of PC that may be facilitate the early diagnosis of PC. Metformin offers a clear benefit of inhibiting PC, whereas insulin therapy may increase the risk of PC development. No evidence has shown that novel hypoglycemic drugs help or prevent PC. In this review, the effects of T2DM on PC development are summarized, and novel strategies for the prevention and treatment of T2DM and PC are discussed.
Therapeutic Applications of Terpenes on Inflammatory Diseases
Authors: Del Prado-Audelo María Luisa , Cortés Hernán , Caballero-Florán Isaac H. , González-Torres Maykel , Escutia-Guadarrama Lidia , Bernal-Chávez Sergio A. , Giraldo-Gomez David M. , Magaña Jonathan J. , Leyva-Gómez Gerardo
Published: August 2021

In the last decades, the search for natural products with biological applications as alternative treatments for several inflammatory diseases has increased. In this respect, terpenes are a family of organic compounds obtained mainly from plants and trees, such as tea, cannabis, thyme, and citrus fruits like lemon or mandarin. These molecules present attractive biological properties such as analgesic and anticonvulsant activities. Furthermore, several studies have demonstrated that certain terpenes could reduce inflammation symptoms by decreasing the release of pro-inflammatory cytokines for example, the nuclear transcription factor-kappa B, interleukin 1, and the tumor necrosis factor-alpha. Thus, due to various anti-inflammatory drugs provoking side effects, the search and analysis of novel therapeutics treatments are attractive. In this review, the analysis of terpenes’ chemical structure and their mechanisms in anti-inflammatory functions are addressed. Additionally, we present a general analysis of recent investigations about their applications as an alternative treatment for inflammatory diseases. Furthermore, we focus on terpenes-based nanoformulations and employed dosages to offer a global perspective of the state-of-the-art.
Ascorbic Acid-Induced Photosynthetic Adaptability of Processing Tomatoes to Salt Stress Probed by Fast OJIP Fluorescence Rise
Authors: Chen Xianjun , Zhou Yan , Cong Yundan , Zhu Pusheng , Xing Jiayi , Cui Jinxia , Xu Wei , Shi Qinghua , Diao Ming , Liu Hui-ying
Published: August 2021

In this study, the protective role of exogenous ascorbic acid (AsA) on salt-induced inhibition of photosynthesis in the seedlings of processing tomatoes under salt stress has been investigated. Plants under salt stress (NaCl, 100 mmol/L) were foliar-sprayed with AsA (0.5 mmol/L), lycorine (LYC, 0.25 mmol/L, an inhibitor of key AsA synthesis enzyme l-galactono-γ-lactone dehydrogenase activity), or AsA plus LYC. The effects of AsA on fast OJIP fluorescence rise curve and JIP parameters were then examined. Our results demonstrated that applying exogenous AsA significantly changed the composition of O-J-I-P fluorescence transients in plants subjected to salt stress both with and without LYC. An increase in basal fluorescence (Fo) and a decrease in maximum fluorescence (Fm) were observed. Lower K- and L-bands and higher I-band were detected on the OJIP transient curves compared, respectively, with salt-stressed plants with and without LYC. AsA application also significantly increased the values of normalized total complementary area (Sm), relative variable fluorescence intensity at the I-step (VI), absorbed light energy (ABS/CSm), excitation energy (TRo/CSm), and reduction energy entering the electron transfer chain beyond QA (ETo/CSm) per reaction centre (RC) and electron transport flux per active RC (ETo/RC), while decreasing some others like the approximated initial slope of the fluorescence transient (Mo), relative variable fluorescence intensity at the K-step (VK), average absorption (ABS/RC), trapping (TRo/RC), heat dissipation (DIo/RC) per active RC, and heat dissipation per active RC (DIo/CSm) in the presence or absence of LYC. These results suggested that exogenous AsA counteracted salt-induced photoinhibition mainly by modulating the endogenous AsA level and redox state in the chloroplast to promote chlorophyll synthesis and alleviate the damage of oxidative stress to photosynthetic apparatus. AsA can also raise the efficiency of light utilization as well as excitation energy dissipation within the photosystem II (PSII) antennae, thus increasing the stability of PSII and promoting the movement of electrons among PS1 and PSII in tomato seedling leaves subjected to salt stress.
Unraveling the Genome of a High Yielding Colombian Sugarcane Hybrid
Authors: Trujillo-Montenegro Jhon Henry , Rodríguez Cubillos María Juliana , Loaiza Cristian Darío , Quintero Manuel , Espitia-Navarro Héctor Fabio , Salazar Villareal Fredy Antonio , Viveros Valens Carlos Arturo , González Barrios Andrés Fernando , De Vega José , Duitama Jorge , Riascos John J.
Published: August 2021

Recent developments in High Throughput Sequencing (HTS) technologies and bioinformatics, including improved read lengths and genome assemblers allow the reconstruction of complex genomes with unprecedented quality and contiguity. Sugarcane has one of the most complicated genomes among grassess with a haploid length of 1Gbp and a ploidies between 8 and 12. In this work, we present a genome assembly of the Colombian sugarcane hybrid CC 01-1940. Three types of sequencing technologies were combined for this assembly: PacBio long reads, Illumina paired short reads, and Hi-C reads. We achieved a median contig length of 34.94 Mbp and a total genome assembly of 903.2 Mbp. We annotated a total of 63,724 protein coding genes and performed a reconstruction and comparative analysis of the sucrose metabolism pathway. Nucleotide evolution measurements between orthologs with close species suggest that divergence between Saccharum officinarum and Saccharum spontaneum occurred <2 million years ago. Synteny analysis between CC 01-1940 and the S. spontaneum genome confirms the presence of translocation events between the species and a random contribution throughout the entire genome in current sugarcane hybrids. Analysis of RNA-Seq data from leaf and root tissue of contrasting sugarcane genotypes subjected to water stress treatments revealed 17,490 differentially expressed genes, from which 3,633 correspond to genes expressed exclusively in tolerant genotypes. We expect the resources presented here to serve as a source of information to improve the selection processes of new varieties of the breeding programs of sugarcane.
Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology
Authors: Bharadwaj Revuru , Kumar Sarma R. , Sharma Ashutosh , Sathishkumar Ramalingam
Published: August 2021

Plants are a remarkable source of high-value specialized metabolites having significant physiological and ecological functions. Genes responsible for synthesizing specialized metabolites are often clustered together for a coordinated expression, which is commonly observed in bacteria and filamentous fungi. Similar to prokaryotic gene clustering, plants do have gene clusters encoding enzymes involved in the biosynthesis of specialized metabolites. More than 20 gene clusters involved in the biosynthesis of diverse metabolites have been identified across the plant kingdom. Recent studies demonstrate that gene clusters are evolved through gene duplications and neofunctionalization of primary metabolic pathway genes. Often, these clusters are tightly regulated at nucleosome level. The prevalence of gene clusters related to specialized metabolites offers an attractive possibility of an untapped source of highly useful biomolecules. Accordingly, the identification and functional characterization of novel biosynthetic pathways in plants need to be worked out. In this review, we summarize insights into the evolution of gene clusters and discuss the organization and importance of specific gene clusters in the biosynthesis of specialized metabolites. Regulatory mechanisms which operate in some of the important gene clusters have also been briefly described. Finally, we highlight the importance of gene clusters to develop future metabolic engineering or synthetic biology strategies for the heterologous production of novel metabolites.
Costunolide Influences Germ Tube Orientation in Sunflower Broomrape – A First Step Toward Understanding Chemotropism
Authors: Krupp Anna , Bertsch Barbara , Spring Otmar
Published: August 2021

Orobanche cumana WALLR. is a host-specific root parasite of cultivated sunflowers with increasing economic importance in Europe, North Africa, and parts of Asia. While sesquiterpene lactones (STLs) released from sunflower roots were identified as natural germination stimulants of O. cumana seeds in the soil, the chemical nature of the signals guiding the emerging germ tube toward the host root has remained unknown hitherto. Thus, we designed a bioassay that allowed the observation of broomrape germination and subsequent germ tube development in the presence of substances with putative chemotropic activity. Root exudates and sunflower oil extracts, both containing STLs in micromolar concentrations, caused the positive chemotropic orientation of germ tubes. A similar positive chemotropic effect was achieved with costunolide, one of the four STLs of sunflower present in the exudate and oil extracts. In contrast, GR24, a synthetic strigolactone (SL) with germination-inducing activity on O. cumana seeds, showed no effect on the germ tube orientation. The effect of costunolide was concentration-dependent and within the range of its natural micromolar occurrence in roots. We assume that an STL gradient is responsible for the stronger inhibition of elongation growth on the host-facing flank of the germ tube compared with the far side flank. This would confer a double role of STLs from sunflower root exudates in the sunflower–broomrape interaction, namely, as germination stimulants and as chemotropic signals.
Potassium and Phosphorus Fertilizer Impacts on Alfalfa Taproot Carbon and Nitrogen Reserve Accumulation and Use During Fall Acclimation and Initial Growth in Spring
Authors: Berg W. Kess , Brouder Sylvie M. , Cunningham Suzanne M. , Volenec Jeffrey J.
Published: August 2021

Phosphorus (P) and potassium (K) impact alfalfa (Medicago sativa L.) performance, but how these nutrients alter taproot physiology during fall acclimation and subsequent growth in spring is unclear. Our objectives were to: (1) determine seasonal patterns for taproot P and K concentrations during fall acclimation and during initial shoot growth in spring; (2) determine how P and K nutrition impacts accumulation of taproot C and N reserves during fall and their subsequent use when shoot growth resumes in spring; and (3) assess how addition of P and K fertilizer impacts survival and shoot growth in spring. Two P (0 and 75 kg ha−1) and two K (0 and 400 kg ha−1) treatments were applied and taproots were sampled between September and December, and again from March to May over 2 years. Concentrations of taproot sugar, starch, buffer-soluble protein, amino-N, and RNA pools were determined. While P and K fertilizer application increased taproot P and K concentrations two- to three-fold, concentrations of P and K in taproots over time did not change markedly during cold acclimation in fall, however, taproot P declined in spring as plant growth resumed. Compared to the 0K-0P treatment, taproots of plants fertilized with 400K-75P had higher starch, protein, amino-N, and RNA, but reduced sugar concentrations in fall. Concentrations of all these pools, except starch, declined during the initial 2 weeks of sampling beginning in late March as shoot growth resumed in spring. Herbage yield in May was highest for the 400K-75P treatment and least for the 0K-0P treatment, differences that were associated with variation in mass shoot−1 and not shoots m−2. High yield of the 400K-75P plants in May was consistently associated with greater concentrations and use of amino-N, soluble protein, and RNA pools in taproots, and not with accumulation and use of starch and sugar pools. Understanding factors leading to the accumulation of taproot N reserves and RNA during cold acclimation in fall and their use during the initial growth in spring should enhance efforts to improve alfalfa growth and herbage yield in spring.
Screening of Olive Biodiversity Defines Genotypes Potentially Resistant to Xylella fastidiosa
Authors: Pavan Stefano , Vergine Marzia , Nicolì Francesca , Sabella Erika , Aprile Alessio , Negro Carmine , Fanelli Valentina , Savoia Michele Antonio , Montilon Vito , Susca Leonardo , Delvento Chiara , Lotti Concetta , Nigro Franco , Montemurro Cinzia , Ricciardi Luigi , De Bellis Luigi , Luvisi Andrea
Published: August 2021

The recent outbreak of the Olive Quick Decline Syndrome (OQDS), caused by Xylella fastidiosa subsp. pauca (Xf), is dramatically altering ecosystem services in the peninsula of Salento (Apulia Region, southeastern Italy). Here we report the accomplishment of several exploratory missions in the Salento area, resulting in the identification of thirty paucisymptomatic or asymptomatic plants in olive orchards severely affected by the OQDS. The genetic profiles of such putatively resistant plants (PRPs), assessed by a selection of ten simple sequence repeat (SSR) markers, were compared with those of 141 Mediterranean cultivars. Most (23) PRPs formed a genetic cluster (K1) with 22 Italian cultivars, including ‘Leccino’ and ‘FS17’, previously reported as resistant to Xf. The remaining PRPs displayed relatedness with genetically differentiated germplasm, including a cluster of Tunisian cultivars. Markedly lower colonization levels were observed in PRPs of the cluster K1 with respect to control plants. Field evaluation of four cultivars related to PRPs allowed the definition of partial resistance in the genotypes ‘Frantoio’ and ‘Nocellara Messinese’. Some of the PRPs identified in this study might be exploited in cultivation, or as parental clones of breeding programs. In addition, our results indicate the possibility to characterize resistance to Xf in cultivars genetically related to PRPs.
The Experience of International Students and Institutional Recommendations: A Comparison Between the Students From the Developing and Developed Regions
Authors: Qadeer Talat , Javed Muhammad Kashif , Manzoor Aqsa , Wu Min , Zaman Syed Imran
Published: August 2021

Prior studies on the experiences of international students in China have mostly focused on their academic, sociocultural, and accommodation experiences. Hence, student health and safety, discrimination, and the services by the International Student Office (ISO) have remained unexplored. Moreover, due to the motivational differences between the students from developing and developed regions, a study that samples students from both regions may depict an exact picture of the experience of international students. Therefore, the objective of this study is to examine the influence of the dimensions (including those dimensions that have been ignored) of the experience of international students on their satisfaction. In addition, we make recommendations regarding Chinese institutes for future students based on a comparison between the students from developing and developed regions. Using hierarchical regression analysis, this study reveals that educational and non-educational experiences vary among students from different regions. Therefore, based on developing (e.g., Asia and Africa) and developed (e.g., America, Europe, and Australia) regions, important recommendations are discussed regarding how educational institutions and the Chinese government could best allocate resources and introduce policies to improve the experience of international students.
The Development of Teachers' and Their Students' Social and Emotional Learning During the “Learning to Be Project”-Training Course in Five European Countries
Authors: Berg Minna , Talvio Markus , Hietajärvi Lauri , Benítez Isabel , Cavioni Valeria , Conte Elisabetta , Cuadrado Francisco , Ferreira Marco , Košir Matej , Martinsone Baiba , Ornaghi Veronica , Raudiene Irena , Šukyte Daiva , Talić Sanela , Lonka Kirsti
Published: August 2021

In recent years, the school curricula in many European countries have introduced social and emotional learning (SEL). This calls for the teachers to have SEL competencies. The present study evaluates teachers' and their students' readiness for SEL during an intervention in five European countries. The participants were teachers (n = 402) in five European countries; Italy, Latvia, Lithuania, Slovenia, and Spain. The pre- and post-measuring points for both the intervention and the comparison group were at approximately the same time before and after the intervention. Comparison data consisted of 159 teachers in the same countries. The training for the intervention group lasted 16 h for the teachers and a maximum of 16 h for the principles and headmasters. An additional 9 h of further monitoring took place. There were two student groups participating in the study: the age group of 8–11 years (pre puberty) and the age group of 12–15-years (adolescents). Students, whose teachers had participated in the intervention, formed the intervention group (n = 2,552). Those students, whose teachers did not participate in the intervention, formed the comparison group (n = 1,730). The questionnaire data were collected at the beginning and at the end of the school year for both age groups. The results indicated that there was a favorable development in the intervention group in some of the measured skills among students, but the effects were different for the two age groups. This study adds to both theoretical and practical development of continuing teacher training about SEL and its possible role in reducing problem behavior among the students.
Are Founders More Socially Responsible? —An Empirical Research on Private Listed Companies in China
Authors: Xiaofei Shi , Xiaoyu Zhou , Lisha Geng
Published: August 2021

Personal factors such as the founder's values and psychological characteristics will influence founder's vision, his perception and interpretation of the decision-making environment, and his strategic choice and decision-making. Based on the theory of entrepreneurial characteristics, combined with the founder's unique psychological characteristics, this paper takes Chinese private listed companies between 2010 and 2018 as a research sample to study the effect of the founder of private enterprises on corporate social responsibility. Furthermore, this study analyzes the impact of different management roles of the founder on corporate social responsibility. We demonstrate that the private enterprises have better social responsibility performance when there are founders; the founders have different management roles, and their corporate social responsibility performance has certain differences, and the higher the management level of the founders, the better the corporate social responsibility performance. This paper studies the issue of corporate social responsibility from the perspective of the characteristics of founders, which expands the current framework of corporate social responsibility research and provides an empirical basis for founders to effectively participate in corporate management in practice.
The Impact of Structural Inequities on Older Asian Americans During COVID-19
Authors: Ma Kris Pui Kwan , Bacong Adrian Matias , Kwon Simona C. , Yi Stella S. , Ðoàn Lan N.
Published: August 2021

Structural racism manifests as an historical and continued invisibility of Asian Americans, whose experiences of disparities and diverse needs are omitted in research, data, and policy. During the pandemic, this invisibility intersects with rising anti-Asian violence and other persistent structural inequities that contribute to higher COVID-19 mortality in older Asian Americans compared to non-Hispanic whites. This perspective describes how structural inequities in social determinants of health—namely immigration, language and telehealth access, and economic conditions—lead to increased COVID-19 mortality and barriers to care among older Asian Americans. Specifically, we discuss how the historically racialized immigration system has patterned older Asian immigrant subpopulations into working in frontline essential occupations with high COVID-19 exposure. The threat of “public charge” rule has also prevented Asian immigrants from receiving eligible public assistance including COVID-19 testing and vaccination programs. We highlight the language diversity among older Asian Americans and how language access remains unaddressed in clinical and non-clinical services and creates barriers to routine and COVID-19 related care, particularly in geographic regions with small Asian American populations. We discuss the economic insecurity of older Asian immigrants and how co-residence in multigenerational homes has exposed them to greater risk of coronavirus transmission. Using an intersectionality-informed approach to address structural inequities, we recommend the disaggregation of racial/ethnic data, meaningful inclusion of older Asian Americans in research and policy, and equitable investment in community and multi-sectoral partnerships to improve health and wellbeing of older Asian Americans.
Global Perspectives of Oral Health Policies and Oral Healthcare Schemes for Older Adult Populations
Authors: Jiang Chloe Meng , Chu Chun Hung , Duangthip Duangporn , Ettinger Ronald L. , Hugo Fernando Neves , Kettratad-Pruksapong Matana , Liu Jian , Marchini Leonardo , McKenna Gerry , Ono Takahiro , Rong Wensheng , Schimmel Martin , Shah Naseem , Slack-Smith Linda , Yang Stella X. , Lo Edward C. M.
Published: August 2021

The aim of this study was to present a concise summary of the oral health policies and oral healthcare schemes for older adult populations in a number of selected countries around the world. In this paper, the current and planned national/regional oral health policies and oral healthcare schemes of nine countries (Australia, Brazil, China including Hong Kong, India, Japan, Switzerland, Thailand, the United Kingdom, and the United States) are reported. Barriers and challenges in oral health promotion in terms of devising oral health policies, implementing oral health schemes, and educating the future dental workforce are discussed. In response to the aging of population, individual countries have initiated or reformed their healthcare systems and developed innovative approaches to deliver oral health services for older adults. There is a global shortage of dentists trained in geriatric dentistry. In many countries, geriatric dentistry is not formally recognized as a specialty. Education and training in geriatric dentistry is needed to produce responsive and competent dental professionals to serve the increasing number of older adults. It is expected that oral health policies and oral healthcare services will be changing and reforming in the coming decades to tackle the enduring oral health challenges of aging societies worldwide.
Unemployment in Socially Disadvantaged Communities in Tennessee, US, During the COVID-19
Authors: Antipova Anzhelika , Momeni Ehsan
Published: August 2021

Urban studies related to previous pandemics and impacts on cities focused on vulnerable categories including poor and marginalized groups. We continue this tradition and analyze unemployment outcomes in a context of a multi-dimensional social disadvantage that is unfolding during the ongoing public health crisis. For this, we first propose an approach to identify communities by social disadvantage status captured by several key metrics. Second, we apply this methodology in the study of the effect of social disadvantage on unemployment during the COVID-19 and measure the COVID-19-related economic impact using the most recent data on unemployment. The study focuses upon vulnerable communities in in the southeastern US (Tennessee) with a concentration of high social vulnerability and rural communities. While all communities initially experienced the impact that was both sudden and severe, communities that had lower social disadvantage pre-COVID were much more likely to start resuming economic activities earlier than communities that were already vulnerable pre-COVID due to high social disadvantage with further implications upon community well-being. The impact of social disadvantage grew stronger post-COVID compared with the pre-pandemic period. In addition, we investigate worker characteristics associated with adverse labor market outcomes during the later stage of the current economic recession. We show that some socio-demographic groups have a systematically higher likelihood of being unemployed. Compared with the earlier stages, racial membership, poverty and loss of employment go hand in hand, while ethnic membership (Hispanics) and younger male workers are not associated with higher unemployment. Overall, the study contributes to a growing contemporaneous research on the consequences of the COVID-19 recession. Motivated by the lack of the research on the spatial aspect of the COVID-19-caused economic recession and its economic impacts upon the vulnerable communities during the later stages, we further contribute to the research gap.
Spatial Perception Enhancement in Assembly Training Using Augmented Volumetric Playback
Authors: Sasikumar Prasanth , Chittajallu Soumith , Raj Navindd , Bai Huidong , Billinghurst Mark
Published: August 2021

Conventional training and remote collaboration systems allow users to see each other’s faces, heightening the sense of presence while sharing content like videos or slideshows. However, these methods lack depth information and a free 3D perspective of the training content. This paper investigates the impact of volumetric playback in a Mixed Reality (MR) spatial training system. We describe the MR system in a mechanical assembly scenario that incorporates various instruction delivery cues. Building upon previous research, four spatial instruction cues were explored; “Annotation”, “Hand gestures”, “Avatar”, and “Volumetric playback”. Through two user studies that simulated a real-world mechanical assembly task, we found that the volumetric visual cue enhanced spatial perception in the tested MR training tasks, exhibiting increased co-presence and system usability while reducing mental workload and frustration. We also found that the given tasks required less effort and mental load when eye gaze was incorporated. Eye gaze on its own was not perceived to be very useful, but it helped to compliment the hand gesture cues. Finally, we discuss limitations, future work and potential applications of our system.
Acute Effects of a Perturbation-Based Balance Training on Cognitive Performance in Healthy Older Adults: A Pilot Study
Authors: Martelli Dario , Kang Jiyeon , Aprigliano Federica , Staudinger Ursula M. , Agrawal Sunil K.
Published: August 2021

Aging is accompanied by an alteration in the capacity to ambulate, react to external balance perturbations, and resolve cognitive tasks. Perturbation-based balance training has been used to induce adaptations of gait stability and reduce fall risk. The compensatory reactions generated in response to external perturbations depend on the activation of specific neural structures. This suggests that training balance recovery reactions should show acute cognitive training effects. This study aims to investigate whether exposure to repeated balance perturbations while walking can produce acute aftereffects that improve proactive and reactive strategies to control gait stability and cognitive performance in healthy older adults. It is expected that an adaptation of the recovery reactions would be associated with increased selective attention and information processing speed. Twenty-eight healthy older adults were assigned to either an Experimental (EG) or a Control Group (CG). The protocol was divided in 2 days. During the first visit, all participants completed the Symbol Digit Modalities Test (SDMT) and the Trail Making Test (TMT). During the second visit, a cable-driven robot was used to apply waist-pull perturbations while walking on a treadmill. The EG was trained with multidirectional perturbations of increasing intensity. The CG walked for a comparable amount of time with cables on, but without experiencing perturbations. Before and after the training, all participants were exposed to diagonal waist-pull perturbations. Changes in gait stability were evaluated by comparing the distance between the heel of the leading leg and the extrapolated Center of Mass (Heel-XCoM Distance—HXD) at perturbation onset (PON) and first compensatory heel strike (CHS). Finally, the cables were removed, and participants completed the SDMT and the TMT again. Results showed that only the EG adapted the gait stability (p < 0.001) in reaction to diagonal perturbations and showed improved performance in the SDMT (p < 0.001). This study provides the first evidence that a single session of perturbation-based balance training produce acute aftereffects in terms of increased cognitive performance and gait stability in healthy older adults. Future studies will include measures of functional activation of the cerebral cortex and examine whether a multi-session training will demonstrate chronic effects.
Assessing Millets and Sorghum Consumption Behavior in Urban India: A Large-Scale Survey
Authors: Kane-Potaka Joanna , Anitha Seetha , Tsusaka Takuji W. , Botha Rosemary , Budumuru Muralidhar , Upadhyay Shweta , Kumar Parkavi , Mallesh Karuna , Hunasgi Raghavendra , Jalagam Ashok Kumar , Nedumaran Swamikannu
Published: August 2021

There is growing attention by governments and industry in regard to the role played by millets (including sorghum) to help build resilience for farmers and cope with climate change, malnutrition, diabetes, and some other major issues. To understand public knowledge and practices of consuming millets in urban areas, a survey was conducted with 15,522 individuals from seven major cities of India using a structured questionnaire, and after data cleaning 15,139 observations were subjected to analysis using descriptive and inferential statistics. It was found that the largest group among early adopters of millets were people with health problems (28%), it being the single largest reason for consuming millets, followed by those wanting to lose weight (15%) and those selecting millets for its taste (14%). There was a significant gap between people who were health conscious (91%) and those who were sure millets were healthy (40%). The major reason the respondents did not eat more millets was that it was not eaten at home (40%), followed by reactions such as not liking the taste (22%). Reaching the urban consumers through social media is recommended, given that it is their main source of information. There was no statistically significant relationship between state-wise per capita production and frequency of consumption of millets in the urban areas (p = 0.236). In conclusion, three key actions are recommended to enhance the consumption of millets: developing delicious products to satisfy the taste, providing knowledge on nutritional and health facts on millets, and improving accessibility of millets in urban markets.
Distributed Secure Edge Computing Architecture Based on Blockchain for Real-Time Data Integrity in IoT Environments
Authors: Xu Rongxu , Hang Lei , Jin Wenquan , Kim Dohyeun
Published: August 2021

The traditional cloud-based Internet of Things (IoT) architecture places extremely high demands on computers and storage on cloud servers. At the same time, the strong dependence on centralized servers causes major trust problems. Blockchain provides immutability, transparency, and data encryption based on safety to solve these problems of the IoT. In this paper, we present a distributed secure edge computing architecture using multiple data storages and blockchain agents for the real-time context data integrity in the IoT environment. The proposed distributed secure edge computing architecture provides reliable access and an unlimited repository for scalable and secure transactions. The architecture eliminates traditional centralized servers using an edge computing framework that represents cloud computing for computer and security issues. Also, blockchain-based edge computing-compatible IoT design is supported to achieve the level of security and scalability required for data integrity. Furthermore, we present the blockchain agent to provide internetworking between blockchain networks and edge computing. For experimenting with the proposed architecture in the IoT environment, we implement and perform a concrete IoT environment based on the EdgeX framework and Hyperledger Fabric. The evaluation results are collected by measuring the performance of the edge computing and blockchain platform based on service execution time to verify the proposed architecture in the IoT environment.
Identification and Validation of QTLs for Yield and Yield Components under Long-Term Salt Stress Using IR64 CSSLs in the Genetic Background of Koshihikari and Their Backcross Progenies
Authors: Mai Nguyen Sao , Hanh Dao Duy , Nakashima Mai , Kumamoto Kotaro , Thuy Nguyen Thi Thu , Kobata Tohru , Saitoh Kuniyuki , Hirai Yoshihiko
Published: August 2021

Unraveling the complex genetic bases and mechanisms underlying salt tolerance is of great importance for developing salt-tolerant varieties. In this study, we evaluated 42 chromosome segment substitution lines (CSSLs) carrying chromosome segments from IR64 on the genetic background of Koshihikari under salt stress. Two CSSLs, SL2007 and SL2038, produced higher plant dry weight and grain yield than did Koshihikari under the stress condition. These CSSLs also showed lower Na+ and Cl− accumulation in the leaf and whole plant at the full heading stage, which might be related to the higher grain yield and yield components. To understand the genetic control of its grain yield and yield components, a SL2007/Koshihikari F2 population was generated for quantitative trait locus (QTL) analysis. Six QTLs for grain yield and yield-related traits were detected on chromosome 2. Using near-isogenic lines (NILs) from a SL2007/Koshihikari F5 population, qSTGY2.2 was delimited to a 2.5 Mb region and novel qSTPN2 was delimited to a 0.6 Mb region. We also detected a novel QTL, qSTGF2, for grain filling, which was considered an important contributor to grain yield under salt stress in this CSSL. Our results provide insights into mechanisms conferring grain yield under salinity stress and new genetic resources for cloning and breeding.
Sub-Lethal Effects of Lecanicillium lecanii (Zimmermann)-Derived Partially Purified Protein and Its Potential Implication in Cotton (Gossypium hirsutum L.) Defense against Bemisia tabaci Gennadius (Aleyrodidae: Hemiptera)
Authors: Abdulle Yusuf Ali , Nazir Talha , Sayed Samy , Mahmoud Samy F. , Majeed Muhammad Zeeshan , Aslam Hafiz Muhammad Usman , Iqbal Zubair , Nisar Muhammad Shahid , Keerio Azhar Uddin , Ali Habib , Qiu Dewen
Published: August 2021

Whiteflies, Bemisia tabaci Gennadius (Aleyrodidae: Hemiptera), are a polyphagous economically destructive pest of several solanaceous crops around the world. Many secondary metabolites are synthesized by different biotrophic and necrotrophic fungi which are capable of inducing systemic resistance in plants against various phytophagous pests. The present laboratory work demonstrated the anti-insect impact of a protein extracted and purified partially from an entomopathogenic fungus (EPF) Lecanicillium lecanii (Zimmermann) against B. tabaci. Three different concentrations (i.e., 7.43, 11.15, and 22.31 μg mL−1) of this protein were bioassayed to assess its effect on the fecundity rate of B. tabaci on cotton (Gossypium hirsutum L.) plants. Furthermore, the possible implication of this fungal protein in defense pathways of cotton plants was evaluated by determining the expression profiles of salicylic acid (SA) and jasmonic acid (JA) pathways related to major genes through reverse transcription qPCR (RT-qPCR). According to the results, all protein concentrations exerted a significant (F3, 252 = 62.51; p ≤ 0.001) and negative impact on the fecundity rate of B. tabaci females. At the highest protein concentration (22.31 μg mL−1), the minimum rate of fecundity (i.e., 2.46 eggs female−1day−1) of B. tabaci was noted on the seventh day, whereas fecundity rates for the other two protein concentrations (i.e., 11.15 and 7.43 μg mL−1) were, respectively, 3.06 and 3.90 eggs day−1 female−1. The maximum rate of fecundity (6.01 eggs female−1day−1) was recorded in untreated (control) treatments. In addition, the foliar application of L. lecanii derived protein significantly upregulated all SA linked genes (OPR3, PPO1 and COI1) and slightly triggered up the JA linked genes (LOX1, UBQ7 and AOS) in the cotton plants. These findings revealed that this L. lecanii extracted partially purified protein triggered systemic resistance against B. tabaci in the cotton plants, proposing its putative effectiveness as an innovative biological control technique against B. tabaci and other phloem-feeding hemipteran pests. Nevertheless, further investigations such as purification and molecular and functional characterization of this L. lecanii-derived partially purified protein are required.
Weeds Spectrum, Productivity and Land-Use Efficiency in Maize-Gram Intercropping Systems under Semi-Arid Environment
Authors: Abbas Rana Nadeem , Arshad Muhammad Awais , Iqbal Asif , Iqbal Muhammad Aamir , Imran Muhammad , Raza Ali , Chen Jen-Tsung , Alyemeni Mohammed Nasser , Hefft Daniel Ingo
Published: August 2021

To ensure food security on sustainable basis, reducing weeds interference and boosting land use efficiency are critical. A field study was conducted at research farm of University of Agriculture Faisalabad, Pakistan, to sort out the most productive maize-gram intercropping system under semi-arid environment. Treatments included sole maize in single row (60 cm apart) (T1) and double rows (90 cm apart) (T2) strips, sole black (T3) and green gram (T4) crops, six single rows (60 cm apart) of maize with twelve double rows (20 cm) of black (T5) and green gram (T6), three double rows (90 cm apart) of maize with three sets of quadratic rows (20 cm apart) of black (T7) and green gram (T8). The experiment was executed in regular arrangement of randomized complete block design with three replications. The results revealed that T1 produced the highest grain yield (6.97 t ha−1) of maize and significantly lower weeds infestation compared to wider row spacing (T2). Among intercropping systems, T8 significantly decreased weeds density (16.33 plants m−2) and their fresh (20.93 g m−2) and dry weights (5.63 g m−2), while the maximum land use efficiency as indicated by unmatched land equivalent ratio and intercropping advantage were recorded by T7 and T8. Interestingly, green gram in intercropping recorded over 58% higher productivity than black gram. We conclude that maize-green gram intercropping hold potential to impart sustainability to maize production by reducing weeds infestation (431% lower than sole maize) and could be a viable option for smallholder farmers in semi-arid environment.
Pre- and Postharvest Management of Sunburn in ‘Granny Smith’ Apples (Malus × domestica Borkh) under Neotropical Climate Conditions
Authors: Severino Vivian , Arias-Sibillotte Mercedes , Dogliotti Santiago , Frins Erna , Yuri José Antonio , González-Talice Jaime
Published: August 2021

Sun damage on apples is attributed to the occurrence of high temperatures, incident radiation, and fruit and plant water parameters, all dependent on climatic conditions and management. The development of new production areas and climate changes increase the interest in studying the behavior of the fruit under different conditions. The effect on sun damage of three nets and two chemical protectants was evaluated in a commercial orchard of ‘Granny Smith’ in a neotropical climate during the 2012–2016 seasons. We recorded the evolution, incidence and severity of sunburn and sunscald. Fruit surface temperature, fruit size, sprouting and return to flowering was also recorded. Incident radiation and air temperature were considered for the assessment of the crop’s microclimate. The transmittance in PAR wavelength and the air temperature variation on netting treatments reached 0.4 and 9 °C, respectively. The fruit surface temperature for the White-Net and Black-Net-50% treatments was always lower than 46 °C. For the sun damaged fruits, the Black-Net-50% treatment showed the highest proportion of slightly sunburned fruits (94%) and the lowest proportion of heavy damages, with a sunscald index equal to or less than 2 (on a scale of 1–4) in all the tested conditions. In a neotropical climate, protectant applications did not reduce the incidence of sunburn, but with the use of nets it was possible to reduce both sunburn and sunscald without affecting growth processes dependent on leaf net assimilation.
Multidrug-Resistant Klebsiella pneumoniae Causing Severe Infections in the Neuro-ICU
Authors: Fursova Nadezhda K. , Astashkin Evgenii I. , Ershova Olga N. , Aleksandrova Irina A. , Savin Ivan A. , Novikova Tatiana S. , Fedyukina Galina N. , Kislichkina Angelina A. , Fursov Mikhail V. , Kuzina Ekaterina S. , Biketov Sergei F. , Dyatlov Ivan A.
Published: August 2021

The purpose of this study was the identification of genetic lineages and antimicrobial resistance (AMR) and virulence genes in Klebsiella pneumoniae isolates associated with severe infections in the neuro-ICU. Susceptibility to antimicrobials was determined using the Vitek-2 instrument. AMR and virulence genes, sequence types (STs), and capsular types were identified by PCR. Whole-genome sequencing was conducted on the Illumina MiSeq platform. It was shown that K. pneumoniae isolates of ST14K2, ST23K57, ST39K23, ST76K23, ST86K2, ST218K57, ST219KL125/114, ST268K20, and ST2674K47 caused severe systemic infections, including ST14K2, ST39K23, and ST268K20 that were associated with fatal incomes. Moreover, eight isolates of ST395K2 and ST307KL102/149/155 were associated with manifestations of vasculitis and microcirculation disorders. Another 12 K. pneumoniae isolates of ST395K2,KL39, ST307KL102/149/155, and ST147K14/64 were collected from patients without severe systemic infections. Major isolates (n = 38) were XDR and MDR. Beta-lactamase genes were identified: blaSHV (n = 41), blaCTX-M (n = 28), blaTEM (n = 21), blaOXA-48 (n = 21), blaNDM (n = 1), and blaKPC (n = 1). The prevalent virulence genes were wabG (n = 41), fimH (n = 41), allS (n = 41), and uge (n = 34), and rarer, detected only in the genomes of the isolates causing severe systemic infections—rmpA (n = 8), kfu (n = 6), iroN (n = 5), and iroD (n = 5) indicating high potential of the isolates for hypervirulence.
MALDI-TOF Mass Spectrometry Technology as a Tool for the Rapid Diagnosis of Antimicrobial Resistance in Bacteria
Authors: Yoon Eun-Jeong , Jeong Seok Hoon
Published: August 2021

Species identification by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a routine diagnostic process for infectious diseases in current clinical settings. The rapid, low-cost, and simple to conduct methodology is expanding its application in clinical microbiology laboratories to diagnose the antimicrobial resistance (AMR) in microorganisms. Primarily, antimicrobial susceptibility testing is able to be carried out either by comparing the area under curve of MALDI spectra of bacteria grown in media with antimicrobial drugs or by identifying the shift peaks of bacteria grown in media including 13C isotope with antimicrobial drugs. Secondly, the antimicrobial resistance is able to be determined through identifying (i) the antimicrobial-resistant clonal groups based on the fingerprints of the clone, (ii) the shift peak of the modified antimicrobial drug, which is inactivated by the resistance determinant, (iii) the shift peak of the modified antimicrobial target, (iv) the peak specific for the antimicrobial determinant, and (v) the biomarkers that are coproduced proteins with AMR determinants. This review aims to present the current usage of the MALDI-TOF MS technique for diagnosing antimicrobial resistance in bacteria, varied approaches for AMR diagnostics using the methodology, and the future applications of the methods for the accurate and rapid identification of AMR in infection-causing bacterial pathogens.