bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach
Authors: Miotto Danyelle S. , Dionizio Aline , Jacomini André M. , Zago Anderson S. , Buzalaf Marília Afonso Rabelo , Amaral Sandra L.
Published: February 2021

Arterial stiffness, frequently associated with hypertension, is associated with disorganization of the vascular wall and has been recognized as an independent predictor of all-cause mortality. The identification of the molecular mechanisms involved in aortic stiffness would be an emerging target for hypertension therapeutic intervention. This study evaluated the effects of perindopril on pulse wave velocity (PWV) and on the differentially expressed proteins in aorta of spontaneously hypertensive rats (SHR), using a proteomic approach. SHR and Wistar rats were treated with perindopril (SHRP) or water (SHRc and Wistar rats) for 8 weeks. At the end, SHRC presented higher systolic blood pressure (SBP, +70%) and PWV (+31%) compared with Wistar rats. SHRP had higher values of nitrite concentration and lower PWV compared with SHRC. From 21 upregulated proteins in the aortic wall from SHRC, most of them were involved with the actin cytoskeleton organization, like Tropomyosin and Cofilin-1. After perindopril treatment, there was an upregulation of the GDP dissociation inhibitors (GDIs), which normally inhibits the RhoA/Rho-kinase/cofilin-1 pathway and may contribute to decreased arterial stiffening. In conclusion, the results of the present study revealed that treatment with perindopril reduced SBP and PWV in SHR. In addition, the proteomic analysis in aorta suggested, for the first time, that the RhoA/Rho-kinase/Cofilin-1 pathway may be inhibited by perindopril-induced upregulation of GDIs or increases in NO bioavailability in SHR. Therefore, we may propose that activation of GDIs or inhibition of RhoA/Rho-kinase pathway could be a possible strategy to treat arterial stiffness.
The Role of Apelin/Apelin Receptor in Energy Metabolism and Water Homeostasis: A Comprehensive Narrative Review
Authors: Hu Gonghui , Wang Zhen , Zhang Rumin , Sun Wenping , Chen Xiaoyu
Published: February 2021

The apelin receptor (APJ) is a member of the family A of G-protein-coupled receptors (GPCRs) and is involved in range of physiological and pathological functions, including fluid homeostasis, anxiety, and depression, as well as cardiovascular and metabolic disorders. APJ was classically described as a monomeric transmembrane receptor that forms a ternary complex together with its ligand and associated G proteins. More recently, increasing evidence indicates that APJ may interact with other GPCRs to form heterodimers, which may selectively modulate distinct intracellular signal transduction pathways. Besides, the apelin/APJ system plays important roles in the physiology and pathophysiology of several organs, including regulation of blood pressure, cardiac contractility, angiogenesis, metabolic balance, and cell proliferation, apoptosis, or inflammation. Additionally, the apelin/APJ system is widely expressed in the central nervous system, especially in neurons and oligodendrocytes. This article reviews the role of apelin/APJ in energy metabolism and water homeostasis. Compared with the traditional diuretics, apelin exerts a positive inotropic effect on the heart, while increases water excretion. Therefore, drugs targeting apelin/APJ system undoubtedly provide more therapeutic options for patients with congestive heart failure accompanied with hyponatremia. To provide more precise guidance for the development of clinical drugs, further in-depth studies are warranted on the metabolism and signaling pathways associated with apelin/APJ system.
Diversified Regulation of Cytokinin Levels and Signaling During Botrytis cinerea Infection in Arabidopsis
Authors: Li Beibei , Wang Ruolin , Wang Shiya , Zhang Jiang , Chang Ling
Published: February 2021

Cytokinins (CKs) can modulate plant immunity to various pathogens, but how CKs are involved in plant defense responses to the necrotrophic pathogen Botrytis cinerea is still unknown. Here, we found that B. cinerea infection induced transcriptional changes in multiple genes involved in the biosynthesis, degradation, and signaling of CKs, as well as their contents, in pathogen-infected Arabidopsis leaves. Among the CKs, the gene expression of CYTOKININ OXIDASE/DEHYDROGENASE 5 (CKX5) was remarkably induced in the local infected leaves and the distant leaves of the same plant without pathogen inoculation. Cis-zeatin (cZ) and its riboside (cZR) accumulated considerably in infected leaves, suggesting an important role of the cis-zeatin type of CKs in the plant response to B. cinerea. Cytokinin double-receptor mutants were more susceptible to B. cinerea infection, whereas an exogenous CK treatment enhanced the expression levels of defense-related genes and of jasmonic acid (JA) and ethylene (ET), but not salicylic acid (SA), resulting in higher resistance of Arabidopsis to B. cinerea. Investigation of CK responses to B. cinerea infection in the JA biosynthesis mutant, jar1-1, and ET-insensitive mutant, ein2-1, showed that CK signaling and levels of CKs, namely, those of isopentenyladenine (iP), isopentenyladenine riboside (iPR), and trans-zeatin (tZ), were enhanced in jar1-1-infected leaves. By contrast, reductions in iP, iPR, tZ, and tZ riboside (tZR) as well as cZR contents occurred in ein2-1-infected leaves, whose transcript levels of CK signaling genes were likewise differentially regulated. The Arabidopsis Response Regulator 5 (ARR5) gene was upregulated in infected leaves of ein2-1 whereas another type-A response regulator, ARR16, was significantly downregulated, suggesting the existence of a complex regulation of CK signaling via the ET pathway. Accumulation of the cis-zeatin type of CKs in B. cinerea-infected leaves depended on ET but not JA pathways. Collectively, our findings provide evidence that CK responds to B. cinerea infection in a variety of ways that are differently modulated by JA and ET pathways in Arabidopsis.
Mitochondrial Small Heat Shock Proteins Are Essential for Normal Growth of Arabidopsis thaliana
Authors: Escobar Mariela R. , Feussner Ivo , Valle Estela M.
Published: February 2021

Mitochondria play important roles in the plant stress responses and the detoxification of the reactive oxygen species generated in the electron transport chain. Expression of genes encoding stress-related proteins such as the mitochondrial small heat shock proteins (M-sHSP) is upregulated in response to different abiotic stresses. In Arabidopsis thaliana, three M-sHSPs paralogous genes were identified, although their function under physiological conditions remains elusive. The aim of this work is to uncover the in vivo function of all three M-sHSPs at the whole plant level. To accomplish this goal, we analyzed the phenotype, proteomic, and metabolic profiles of Arabidopsis knock-down lines of M-sHSPs (single, double, and triple knock-down lines) during normal plant growth. The triple knock-down plants showed the most prominent altered phenotype at vegetative and reproductive stages without any externally applied stress. They displayed chlorotic leaves, growth arrest, and low seed production. Concomitantly, they exhibited increased levels of sugars, proline, and citric, malic, and ascorbic acid, among other metabolites. In contrast, single and double knock-down plants displayed a few changes in their phenotype. A redundant function among the three M-sHSPs is indicated by the impairment in vegetative and reproductive growth associated with the simultaneous loss of all three M-sHSPs genes. The triple knock-down lines showed alteration of proteins mainly involved in photosynthesis and antioxidant defense compared to the control plants. On the other hand, heat stress triggered a distinct cytosolic response pattern and the upregulation of other sHSP members, in the knock-down plants. Overall, depletion of all three M-sHSPs in Arabidopsis severely impacted fundamental metabolic processes, leading to alterations in the correct plant growth and development. These findings expand our knowledge about the contribution of organelle-specific M-sHSPs to healthy plant growth under non-stress conditions.
Development and Validation of Methodology for Estimating Potato Canopy Structure for Field Crop Phenotyping and Improved Breeding
Authors: de Jesus Colwell Filipe , Souter Jock , Bryan Glenn J. , Compton Lindsey J. , Boonham Neil , Prashar Ankush
Published: February 2021

Traditional phenotyping techniques have long been a bottleneck in breeding programs and genotype- phenotype association studies in potato, as these methods are labor-intensive and time consuming. In addition, depending on the trait measured and metric adopted, they suffer from varying degrees of user bias and inaccuracy, and hence these challenges have effectively prevented the execution of large-scale population-based field studies. This is true not only for commercial traits (e.g., yield, tuber size, and shape), but also for traits strongly associated with plant performance (e.g., canopy development, canopy architecture, and growth rates). This study demonstrates how the use of point cloud data obtained from low-cost UAV imaging can be used to create 3D surface models of the plant canopy, from which detailed and accurate data on plant height and its distribution, canopy ground cover and canopy volume can be obtained over the growing season. Comparison of the canopy datasets at different temporal points enabled the identification of distinct patterns of canopy development, including different patterns of growth, plant lodging, maturity and senescence. Three varieties are presented as exemplars. Variety Nadine presented the growth pattern of an early maturing variety, showing rapid initial growth followed by rapid onset of senescence and plant death. Varieties Bonnie and Bounty presented the pattern of intermediate to late maturing varieties, with Bonnie also showing early canopy lodging. The methodological approach used in this study may alleviate one of the current bottlenecks in the study of plant development, paving the way for an expansion in the scale of future genotype-phenotype association studies.
Development of the Middle Layer in the Anther of Arabidopsis
Authors: Xue Jing-Shi , Yao Chi , Xu Qin-Lin , Sui Chang-Xu , Jia Xin-Lei , Hu Wen-Jing , Lv Yong-Lin , Feng Yi-Feng , Peng Yu-Jia , Shen Shi-Yi , Yang Nai-Ying , Lou Yu-Xia , Yang Zhong-Nan
Published: February 2021

The middle layer is an essential cell layer of the anther wall located between the endothecium and tapetum inArabidopsis. Based on sectioning, the middle layer was found to be degraded at stage 7, which led to the separation of the tapetum from the anther wall. Here, we established techniques for live imaging of the anther. We created a marker line with fluorescent proteins expressed in all anther layers to study anther development. Several staining methods were used in the intact anthers to study anther cell morphology. We clarified the initiation, development, and degradation of the middle layer inArabidopsis. This layer is initiated from both the inner and outer secondary parietal cells at stage 4, stopped cell division at stage 6, and finally degraded at stage 11. The neighboring cell layers, the epidermis, and endothecium continued cell division until stage 10, which led to a thin middle layer. The degradation of the tapetum cell wall at stage 7 lead to its isolation from the anther wall. This work presents fundamental information on the development of the middle layer, which facilitates the further investigation of anther development and plant fertility. These live imaging methods could be useful in future studies.
Specificity and Plasticity of the Functional Ionome of Brassica napus and Triticum aestivum Exposed to Micronutrient or Beneficial Nutrient Deprivation and Predictive Sensitivity of the Ionomic Signatures
Authors: D’Oria Aurélien , Courbet Galatéa , Lornac Aurélia , Pluchon Sylvain , Arkoun Mustapha , Maillard Anne , Etienne Philippe , Diquélou Sylvain , Ourry Alain
Published: February 2021

The specific variation in the functional ionome was studied in Brassica napus and Triticum aestivum plants subjected to micronutrient or beneficial mineral nutrient deprivation. Effects of these deprivations were compared to those of macronutrient deprivation. In order to identify early events, plants were harvested after 22 days, i.e., before any significant reduction in growth relative to control plants. Root uptake, tissue concentrations and relative root nutrient contents were analyzed revealing numerous interactions with respect to the 20 elements quantified. The assessment of the functional ionome under individual mineral nutrient deficiency allows the identification of a large number of interactions between elements, although it is not totally exhaustive, and gives access to specific ionomic signatures that discriminate among deficiencies in N, P, S, K, Ca, Mn, Fe, Zn, Na, Si, and Se in both species, plus Mg, Cl, Cu, and Mo in wheat. Ionome modifications and components of ionomic signatures are discussed in relation to well-known mechanisms that may explain crosstalks between mineral nutrients, such as between Na and K, V, Se, Mo and S or Fe, Zn and Cu. More surprisingly, when deprived of beneficial nutrients such as Na, Si, Co, or Se, the plant ionome was strongly modified while these beneficial nutrients contributed greatly to the leaf ionomic signature of most mineral deficiencies.
The Effects of COVID-19 Lockdown 1.0 on Working Patterns, Income, and Wellbeing Among Performing Arts Professionals in the United Kingdom (April–June 2020)
Authors: Spiro Neta , Perkins Rosie , Kaye Sasha , Tymoszuk Urszula , Mason-Bertrand Adele , Cossette Isabelle , Glasser Solange , Williamon Aaron
Published: February 2021

This article reports data collected from 385 performing arts professionals using the HEartS Professional Survey during the COVID-19 Lockdown 1.0 in the United Kingdom. Study 1 examined characteristics of performing arts professionals’ work and health, and investigated how these relate to standardized measures of wellbeing. Study 2 examined the effects of the lockdown on work and wellbeing in the respondents’ own words. Findings from Study 1 indicate a substantial reduction in work and income. 53% reported financial hardship, 85% reported increased anxiety, and 63% reported being lonelier than before the crisis. 61% sought support on finances while only 45% did so on health and wellbeing. Multiple regression analyses, using the Mental Health Continuum-Short Form, Center for Epidemiologic Studies Depression Scale, Social Connectedness Scale, and Three-Item Loneliness Scale as outcome variables, indicate that perceived financial hardship was associated with lower wellbeing and higher depression and loneliness scores. Higher self-rated health was associated with higher wellbeing and lower depression scores. More physical activity before lockdown was associated with higher wellbeing and social connectedness scores, as well as lower loneliness scores, and an increase in physical activity during lockdown compared with before, as well as older age, were associated with higher wellbeing and social connectedness scores, and lower depression and loneliness scores. Thematic inductive analysis of 341 open responses in Study 2 identified five overarching themes characterizing the effects of Lockdown 1.0: lost or uncertain work and income, including canceled work, financial concerns, and uncertainties for the future; constraints of lockdown working, including challenges of working at home, struggles with online work and skill maintenance, and caring responsibilities; loss and vulnerability, including reduced social connections, lack of support, vulnerability, feelings of loss and grief, and concern for others; detrimental effects on health and wellbeing, including anxiety, low or unstable mood, poorer physical health, and lack of motivation; and professional and personal opportunities, including coping well or living more healthily, more time and less pressure, new possibilities and activities, enhanced social connections, and new skills. Lockdown 1.0 had profound effects on performing arts professionals, but our findings reveal some opportunities and compelling links between positive wellbeing and physical activity.
It’s All Up to My Fellow Citizens. Descriptive Norms as a Decisive Mediator in the Relationship Between Infrastructure and Mobility Behavior
Authors: Rollin Philipp , Bamberg Sebastian
Published: February 2021

Following the implementation of temporary pop-up bike lanes in Berlin, traffic counts by the city administration show an increased number of cyclists. This present paper aims to understand reasons behind this observation. To this end, we focus on the role of mobility-related descriptive social norms as mediators of this effect. Results from one correlational and two experimental online studies are reported. The correlational study confirms the expected association of mobility-related descriptive social norms and self-reported mobility behavior. Moreover, it demonstrates that, as expected, mobility-related descriptive social norms reliably reflect differences in cities’ objective transport structure and mediate the impact of these infrastructural differences on mobility behavior. Results from two online experiments provide additional causal evidence that participants use the visual cues provided by manipulated photos to form their perceived mobility-related descriptive social norms. Furthermore, the second online experiment provides evidence that the combination of infrastructural cues and observable mobility behavior has the strongest impact on perceived mobility-related descriptive social norms.
The Impact of Coronavirus Disease 2019 Lockdown on Athletes’ Subjective Vitality: The Protective Role of Resilience and Autonomous Goal Motives
Authors: Martínez-González Natalia , Atienza Francisco L. , Tomás Inés , Duda Joan L. , Balaguer Isabel
Published: February 2021

The lockdown resulting from coronavirus disease 2019 (COVID-19) has had a huge impact on peoples’ health. In sport specifically, athletes have had to deal with frustration of their objectives and changes in their usual training routines. The challenging and disruptive situation could hold implications for their well-being. This study examined the effect of the COVID-19 lockdown on changes in athletes’ reported eudaimonic well-being (subjective vitality) and goal motives (autonomous and controlled) over time (i.e., pre-lockdown and during lockdown). The relationship of resilience to changes in subjective vitality was also determined, and changes in athletes’ goal motives were examined as potential mediators. Participants were 127 Spanish university athletes aged between 18 and 34 years (M = 21.14; SD = 2.77). Approximately 4 months before the start of the lockdown in Spain (T1), athletes responded to a questionnaire assessing their resilience, goal motives, and subjective vitality. Around 6 months later into the lockdown period (T2), athletes’ goal motives and subjective vitality were assessed again. Growth modeling using hierarchical linear models revealed a significant decrease of autonomous goal motives and subjective vitality during the lockdown, but athletes did not show change over time in controlled goal motives. Path analysis, adjusting T2 measures for their corresponding T1 measures, showed that resilience significantly predicted changes in athletes’ autonomous goal motives, which then accounted for changes in subjective vitality. The indirect effect was significant. Resilience did not predict changes in athletes’ controlled goal motives. However, changes in controlled goal motives negatively predicted changes in subjective vitality during lockdown. The findings suggest negative impacts of the COVID-19 lockdown on athletes’ goal motives and eudaimonic well-being. Results also support the hypothesized mediational role of autonomous goal motives in the relationship between resilience and subjective vitality during the lockdown. As such, findings confirm the relevance of resilience to a key feature of athletes’ eudaimonic well-being and the importance of enhancing their autonomous goal striving.
The Analysis of Mathematics Academic Burden for Primary School Students Based on PISA Data Analysis
Authors: Wang Li
Published: February 2021

To explore the impact of academic burden on the physical and mental health of primary school students, combined with the results of the Programme for International Student Assessment (PISA) report in 2018, the relationship among the development of mathematical literacy, mathematics academic burden, and the physical and mental health of primary school students is studied. First, the relationship between mathematical literacy and mathematics anxiety is analyzed, and related influencing factors and measurement methods of mathematics anxiety are introduced. A questionnaire is then designed for primary school students’ mathematical stress, and the reliability and validity of the designed questionnaire are tested. Finally, a questionnaire survey is conducted on students, parents, and teachers in the third, fourth, and fifth grades of three standardized public primary schools. The results of the questionnaire survey show that students, teachers, and parents have a general understanding of the mathematics academic burden of primary school students at this stage. A total of 70% of teachers believe that primary school students have a heavy mathematics burden; 50% of parents think that primary school students are under heavy academic stress; 70% of primary school students believe that the heavy mathematics burden leads to reduced sleep time and extracurricular activities, which has a serious impact on the physical and mental health of primary school students. This research provides a reference for improving the current balance between education and students’ physical and mental health in China.
INSPIRED but Tired: How Medical Faculty’s Job Demands and Resources Lead to Engagement, Work-Life Conflict, and Burnout
Authors: Lee Rebecca S. , Son Hing Leanne S. , Gnanakumaran Vishi , Weiss Shelly K. , Lero Donna S. , Hausdorf Peter A. , Daneman Denis
Published: February 2021

Background

Past research shows that physicians experience high ill-being (i.e., work-life conflict, stress, burnout) but also high well-being (i.e., job satisfaction, engagement).

Objective

To shed light on how medical faculty’s experiences of their job demands and job resources might differentially affect their ill-being and their well-being with special attention to the role that the work-life interface plays in these processes.

Methods

Qualitative thematic analysis was used to analyze interviews from 30 medical faculty (19 women, 11 men, average tenure 13.36 years) at a top research hospital in Canada.

Findings

Medical faculty’s experiences of work-life conflict were severe. Faculty’s job demands had coalescing (i.e., interactive) effects on their stress, work-life conflict, and exhaustion. Although supportive job resources (e.g., coworker support) helped to mitigate the negative effects of job demands, stimulating job resources (e.g., challenging work) contributed to greater work-life conflict, stress, and exhaustion. Thus, for these medical faculty job resources play a dual-role for work-life conflict. Moreover, although faculty experienced high emotional exhaustion, they did not experience the other components of burnout (i.e., reduced self-efficacy, and depersonalization). Some faculty engaged in cognitive reappraisal strategies to mitigate their experiences of work-life conflict and its harmful consequences.

Conclusion

This study suggests that the precise nature and effects of job demands and job resources may be more complex than current research suggests. Hospital leadership should work to lessen unnecessary job demands, increase supportive job resources, recognize all aspects of job performance, and, given faculty’s high levels of work engagement, encourage a climate that fosters work-life balance.
On the Multimodal Path to Language: The Relationship Between Rhythmic Movements and Deictic Gestures at the End of the First Year
Authors: Murillo Eva , Montero Ignacio , Casla Marta
Published: February 2021

The aim of this study is to analyze the relationship between rhythmic movements and deictic gestures at the end of the first year of life, and to focus on their unimodal or multimodal character. We hypothesize that multimodal rhythmic movement performed with an object in the hand can facilitate the transition to the first deictic gestures. Twenty-three children were observed at 9 and 12 months of age in a naturalistic play situation with their mother or father. Results showed that rhythmic movements with objects in the hand are a frequent behavior in children's repertoires. Rhythmic behaviors tend to decrease from 9 to 12 months, specifically when they are unimodal. Multimodal rhythmic behavior production at 9 months is positively related with proximal deictic gestures 3 months later. Multimodal rhythmic movements are not directly related to distal deictic gestures, but are indirectly related via proximal deictic gestures. These results highlight the relevance of multimodal behaviors in the transition to the use of early gestures, and can be considered as a transitional phenomenon between the instrumental action and early communicative gestures.
Male Mating Expectations in Brazilian and American Samples
Authors: Castro Felipe Nalon , Hattori Wallisen Tadashi , Gaulin Steven J. C. , Yamamoto Maria Emília , Lopes Fívia de Araújo
Published: February 2021

This study aims to investigate assortative mating based on mate value from male perspective. Male participants (132 Brazilian and 106 American) evaluated hypothetical “stimulus” males described in terms of physical attractiveness, social skills, and social status (each varied in high or low levels). Participants rated each stimulus and each stimulus' preferred mating partner on nine traits. The results showed that (1) positive assortative mating was expected in romantic relationships; (2) the stimulus ratings did not vary independently, suggesting that mate value is the result of the interaction of the characteristics of individuals; and (3) that participants expected physically attractive and healthier female partners to pair with high-status male stimuli. The American and Brazilian mating expectations were similar, minor differences indicate that Brazilian participants considered men with high levels of social skills to be more ambitious and intelligent; American participants expected men of high status to be healthier; Brazilians expect men of high status to have more attractive faces, while Americans expected these men to possess more attractive bodies; and Brazilian participants assigned more attractive bodies to men of lower status. These differences reflect the influence of economic and cultural factors on the local environment. The study contributes to the understanding of the construction of market value and reveals that male expectations are in line with human mating preferences. The investigation of mating expectations can be a rich approach to investigate socio-cultural aspects that are related to mating in different cultures
Prevalence and Factors Associated With Surgical Site Infections in the University Clinics of Traumatology and Urology of the National University Hospital Centre Hubert Koutoukou Maga in Cotonou
Authors: Dégbey Cyriaque , Kpozehouen Alphonse , Coulibaly Drissa , Chigblo Pascal , Avakoudjo Josué , Ouendo Edgard-Marius , Hans-Moevi Aristote
Published: February 2021

Objectives: Study the prevalence and factors associated with the occurrence of surgical site infections in University Clinics of Trauma-Orthopedics, Reconstructive Surgery and Urology in a developing country.

Methods: This was a retrospective descriptive and analytical study of 384 people operated on during the period of 2019. Logistic regression was used to study the factors associated with surgical site infections. The associations between the dependent variable and the other variables were assessed by the odds ratio (OR) followed by their 95% confidence interval.

Results: The prevalence of surgical site infections was 7.81% CI 95% = (5.12–10.51). The factors linked to the surgical site infections in the studied population were the patient's admission method [OR = 2.74; 95% CI = (1.08–6.95)] and the length of the postoperative stay [OR = 8.75; 95% CI = (2.83–26.98)]. The interview and direct observation identified health care system dysfunctions, medical errors, patient monitoring and financial unavailability as factors that could favor the onset of surgical site infections.

Conclusion: Interventions should be focused on the factors identified for the effective management of operated patients.
Accumulative Competitive Season Training Stress Affects Neuromuscular Function and Increases Injury Risk in Uninjured D1 Female Athletes
Authors: Purdom Troy M. , Levers Kyle S. , Giles Jacob , Brown Lindsey , McPherson Chase S. , Howard Jordan
Published: February 2021

Previous research has shown that acute competition training stress negatively affects neuromuscular function which can perpetuate a predisposition to injury. This study's aim was to investigate the effect of accumulated competition training stress effect on neuromuscular function and incidence of increased injury risk in uninjured female D1 soccer players. Neuromuscular function was evaluated in fifteen female division I soccer athletes who played >85% of competitive season competitions who were tested for mobility/stability, leg length symmetry, and vertical power at three different points across the competitive season (pre, mid, and post time blocks). Leg length symmetry was measured from the anterior superior iliac spine to the lateral malleolus prior to Y-balance testing. The Y-balance testing measures unilateral anterior, posteromedial, and posterolateral reach achieved in single leg stance using metrics that include L/R normalized composite reach (NCOMP), L/R normalized antiorior reach (NANT), and L/R NCOMP/NANT segmental differences across time. Injury risk was evaluated using validated objective criteria that included: (NCOMP total reach <94% of limb length*3), (NANT reach distance <84% leg length) along with NCOMP and NANT asymmetries >4.0. Maximal vertical power (MVP) was measured via vertical jump. Multiple repeated measures ANOVAs evaluated NCOMP, NANT, MVP, and leg length symmetry across time with LSD post hoc testing when relevant (X ± SD). A significant main effect was found [F(1, 14) = 62.92, p < 0.001; η2 =0.82] with training stress and neuromuscular function without affecting maximal vertical power. Eighty percent of subject's bilateral NCOMP scores fell below the YBT reach standard at midseason (ES = 0.95, p = 0.02) while all subjects NANT reach distance remained below the reach threshold (ES = 0.74, p = 0.003) indicating a 6.5× and 2.5× greater injury risk, respectively. Competition stress affected neuromuscular function without affecting maximal power, which negatively impacted stability and increased injury risk.
Method to Investigate Multi-Axis Release Action of Ski Safety Bindings: A New Approach for Testing in Research and Development
Authors: Nimmervoll Florian , Eckerstorfer Roland , Braumann Johannes , Petutschnigg Alexander , Sternad Bruno
Published: February 2021

The authors developed and elaborated on a new method to release ski bindings by utilizing an industrial robot to simulate release movement showing a spatial repeatability of ± 0.06 mm. The parametric programming of the release parameters gave free control while executing repeatable release tests. A series of different motion patterns were performed, on the one hand, to test the applicability of the setup to the simulation of motion patterns and, on the other, to check for the impact of the ski deformations like ski deflections within the range of −5 mm to −85 mm, on the safety bindings' release forces. As certain falling mechanisms are related to knee injury, which is the most common severe injury in alpine skiing, this testing method can be used to develop related displacement movements in future. This movements do not necessarily accord with the directional release mechanics of safety ski bindings. The authors specify the developed testing apparatus as device for force measurements in 3D with an accuracy of ± 0.5% in boot-sole-plane. The intention behind this development is to enable faster, more versatile and adaptive testing procedures in R&D.
Smart Black Box 2.0: Efficient High-Bandwidth Driving Data Collection Based on Video Anomalies
Authors: Feng Ryan , Yao Yu , Atkins Ella
Published: February 2021

Autonomous vehicles require fleet-wide data collection for continuous algorithm development and validation. The smart black box (SBB) intelligent event data recorder has been proposed as a system for prioritized high-bandwidth data capture. This paper extends the SBB by applying anomaly detection and action detection methods for generalized event-of-interest (EOI) detection. An updated SBB pipeline is proposed for the real-time capture of driving video data. A video dataset is constructed to evaluate the SBB on real-world data for the first time. SBB performance is assessed by comparing the compression of normal and anomalous data and by comparing our prioritized data recording with an FIFO strategy. The results show that SBB data compression can increase the anomalous-to-normal memory ratio by ∼25%, while the prioritized recording strategy increases the anomalous-to-normal count ratio when compared to an FIFO strategy. We compare the real-world dataset SBB results to a baseline SBB given ground-truth anomaly labels and conclude that improved general EOI detection methods will greatly improve SBB performance.
Rootstock Affects Floral Induction in Citrus Engaging the Expression of the FLOWERING LOCUS T (CiFT)
Authors: Bennici Stefania , Las Casas Giuseppina , Distefano Gaetano , Gentile Alessandra , Lana Giuseppe , Di Guardo Mario , Nicolosi Elisabetta , La Malfa Stefano , Continella Alberto
Published: February 2021

In Citrus, flower induction represents the transition from vegetative to reproductive growth. The regulation of flower induction is mainly triggered by exposure to low temperatures and water-deficit stress, which activates the signaling cascade leading to an increased expression of the citrus orthologs of the FLOWERING LOCUS T (CiFT). In this study, the relationship between rootstock and flower induction under Mediterranean field conditions was investigated by monitoring the expression levels of the floral promoter CiFT2 in leaves of the pigmented sweet orange “Tarocco Scirè” grafted onto “C35” citrange and “Swingle” citrumelo rootstocks. The latter two are known to confer, respectively, high and low yield efficiency to the scion. In both rootstock/scion combinations, CiFT2 showed a seasonal expression with a peak during the inductive period in January triggered by cold temperature. The “Tarocco Scirè”/”C35” citrange combination showed the highest expression levels for CiFT2; this increased expression was correlated with yield and a higher number of flowers in the following spring, suggesting a significant effect of rootstocks on flower induction mediated by the overexpression of the CiFT2 gene.
Quanti-Qualitative Response of Swiss Chard (Beta vulgaris L. var. cycla) to Soil Amendment with Biochar-Compost Mixtures
Authors: Libutti Angela , Rivelli Anna Rita
Published: February 2021

In recent years, soil addition with organic amendments, such as biochar and compost, has gained attention as an effective agronomic practice to sustain soil fertility, enhance plant growth and crop yield. Well known are the positive effects of compost on yield of a wide crop varieties, while both positive and negative responses are reported for biochar Therefore, the aim of the study was to verify the effect of biochar mixed with three types of compost on quanti-qualitative response of Swiss chard (Beta vulgaris L. cycla), a leafy green vegetable rich in dietary antioxidants, largely consumed worldwide. A factorial experiment in pots with two factors, including biochar (without biochar and with biochar from vine pruning residues) and compost (without compost, with compost from olive pomace, with vermicompost from cattle manure, and with compost from cattle anaerobic digestate), was setup. Two growth cycles were considered, and a set of quantitative (height of plants, number, area and fresh weight of leaves) and qualitative parameters (carotenoids, chlorophyll, total N, and NO3−content of leaves) were analyzed. Biochar decreased plant growth and NO3− leaf content; on the contrary, it increased total N leaf content, while compost improved all the considered parameters. The interactive effect of biochar and compost was evident only on total N and NO3− leaf content. In our experimental conditions, the compost showed to be the best option to improve Swiss chard growth and increase the content of phytopigments, while the biochar-compost mixtures did not produce the expected effect.
Foliar Spray Application of Chlorella vulgaris Extract: Effect on the Growth of Lettuce Seedlings
Authors: La Bella Emanuele , Baglieri Andrea , Rovetto Ermes Ivan , Stevanato Piergiorgio , Puglisi Ivana
Published: February 2021

Lettuce seedlings often require the use of fertilizers for their cultivation management to achieve appropriate yield. However, for eco-sustainable chemical-fertilizers-free agronomy, the implementation of totally organic farming often cannot support lettuce productivity, therefore new natural biostimulants able to increase lettuce yield could be considered of great interest. In this preliminary work, the foliar spray application of a Chlorella vulgaris extract in lettuce seedlings was investigated in order to achieve better yield performance. Its biostimulant effect was evaluated by monitoring the morphobiometric parameters, chlorophylls, carotenoids, total protein contents, and several enzymatic activities involved in primary and secondary metabolisms of the plant. The experimental trials were carried out by growing lettuce seedlings on inert substrate (pumice) with a 16 h photoperiod for 21 days. The treatment consisted of three consecutive applications by foliar spraying using a concentration of the C. vulgaris extract, corresponding to 1 mg Corg L−1, which were performed one week apart. The results showed that the C. vulgaris extract positively influenced the growth of lettuce seedlings, by increasing the fresh and dry weights, chlorophylls, carotenoids, protein content, and ashes at shoot level. From a biochemical point of view, primary and secondary metabolisms of shoots, in particular nitrogen metabolism, were positively influenced. At the root level, the extract increased dry matter, proteins, and ash content.
Testing the Suitability of Automated Machine Learning for Weeds Identification
Authors: Espejo-Garcia Borja , Malounas Ioannis , Vali Eleanna , Fountas Spyros
Published: February 2021

In the past years, several machine-learning-based techniques have arisen for providing effective crop protection. For instance, deep neural networks have been used to identify different types of weeds under different real-world conditions. However, these techniques usually require extensive involvement of experts working iteratively in the development of the most suitable machine learning system. To support this task and save resources, a new technique called Automated Machine Learning has started being studied. In this work, a complete open-source Automated Machine Learning system was evaluated with two different datasets, (i) The Early Crop Weeds dataset and (ii) the Plant Seedlings dataset, covering the weeds identification problem. Different configurations, such as the use of plant segmentation, the use of classifier ensembles instead of Softmax and training with noisy data, have been compared. The results showed promising performances of 93.8% and 90.74% F1 score depending on the dataset used. These performances were aligned with other related works in AutoML, but they are far from machine-learning-based systems manually fine-tuned by human experts. From these results, it can be concluded that finding a balance between manual expert work and Automated Machine Learning will be an interesting path to work in order to increase the efficiency in plant protection.
Chronological Expression of PITX2 and SIX1 Genes and the Association between Their Polymorphisms and Chicken Meat Quality Traits
Authors: Cao Haiyue , Zhou Wei , Tan Yuge , Xu Xiuli , Mao Haiguang , Dong Xinyang , Xu Ningying , Yin Zhaozheng
Published: February 2021

Meat quality is closely related to the development of skeletal muscle, in which PITX2 and SIX1 genes play important regulatory roles. The present study firstly provided the data of chronological expression files of PITX2 and SIX1 genes in the post-hatching pectoral muscle and analyzed the association of their polymorphisms with the meat quality traits of Wuliang Mountain Black-bone (WLMB) chickens. The results showed that both PITX2 and SIX1 genes were weakly expressed in the second and third weeks, and then increased significantly from the third week to the fourth week. Furthermore, there was a significant positive correlation between the expression levels of the two genes. Twelve and one SNPs were detected in the chicken PITX2 and SIX1 genes, respectively, of which four SNPs (g.9830C > T, g.10073C > T, g.13335G > A, g.13726A > G) of the PITX2 gene and one SNP (g.564G > A) of the SIX1 gene were significantly associated with chicken meat quality traits. For the PITX2 gene, chickens with the CT genotype of g.9830C > T showed the highest meat color L*, shear force (SF), pH, and the lowest electrical conductivity (EC), and drip loss (DL) (p < 0.05 or p < 0.01); chickens with the CC genotype of g.10073C > T had the lowest L*, pH, and the highest DL (p < 0.01). For the SIX1 gene, chickens with the GG genotype of g.564G > A had the highest (p < 0.05) SF and pH. Furthermore, pH had a significant correlation with all the other meat quality traits. The current study could contribute to the research of regulatory mechanisms of meat quality and lay the foundation for improving meat quality based on marker-assisted selection in chickens.
Glutamic Acid Decarboxylase Concentration Changes in Response to Stress and Altered Availability of Glutamic Acid in Rabbit (Oryctolagus cuniculus) Brain Limbic Structures
Authors: Szpręgiel Izabela , Wrońska Danuta , Kmiecik Michał , Pałka Sylwia , Kania Bogdan F.
Published: February 2021

Glutamic acid decarboxylase (GAD) is an enzyme that catalyses the formation of γ-aminobutyric acid (GABA), the most important inhibitory neurotransmitter, from glutamic acid (Glu), which is considered the most important excitatory transmitter in the central and peripheral nervous systems. GAD is a key enzyme that provides a balance between Glu and GABA concentration. Hence, it can be assumed that if the GAD executes the synthesis of GABA from Glu, it is important in the stress response, and thus also in triggering the emotional states of the body that accompany stress. The aim of the study was to investigate the concentration of the GAD in motivational structures in the brain of the rabbit (Oryctolagus cuniculus) under altered homeostatic conditions caused by stress and variable availability of Glu. Summarising, the experimental results clearly showed variable concentrations of GAD in the motivational structures of the rabbit brain. The highest concentration of GAD was found in the hypothalamus, which suggests a strong effect of Glu and GABA on the activity of this brain structure. The GAD concentrations in individual experimental groups depended to a greater extent on blocking the activity of glutamate receptors than on the effects of a single stress exposure. The results obtained clearly support the possibility that a rapid change in the concentration of GAD could shift bodily responses to quickly achieve homeostasis, especially in this species. Further studies are necessary to reveal the role of the Glu–GAD–GABA system in the modulation of stress situations as well as in body homeostasis.
Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface
Authors: Schmitt Kira , Kuster Stefan P. , Zurfluh Katrin , Jud Rahel S. , Sykes Jane E. , Stephan Roger , Willi Barbara
Published: February 2021

Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) among animals and humans are a public health threat. This study analyzed the occurrence of ESBL-E in a high-risk environment in a companion animal clinic and two animal patients’ households. In an intensive care unit (ICU), rectal swabs from 74 dogs and cats, 74 hand swabs from staff and 298 swabs from surfaces were analyzed for ESBL-E. Seventeen hospitalized patients (23%) and ten (3%) surfaces in the ICU tested ESBL-E positive. Transmission chains for Klebsiella pneumoniae ST307 blaCTX-M-15 and Escherichia coli ST38 blaCTX-M-14, ST88 blaCTX-M-14 and ST224 blaCTX-M-1 were observed over extended periods of time (14 to 30 days) with similar strains isolated from patients and the clinical environment. After discharge, two colonized dogs (dogs 7 and 12) and their household contacts were resampled. Dog 7 tested repeatedly positive for 77 days, dog 12 tested negative; six (24%) surfaces in the household of the persistently colonized dog tested ESBL-E positive. The owner of dog 7 and one of the owners of dog 12 were colonized. Based on whole genome sequencing, isolates from the owners, their dogs and other ICU patients belonged to the same clusters, highlighting the public health importance of ESBL-E in companion animal clinics.
An Aerodynamic Design Method to Improve the High-Speed Performance of a Low-Aspect-Ratio Tailless Aircraft
Authors: Liu Zhongyuan , Luo Lie , Zhang Binqian
Published: February 2021

This paper puts forward an aerodynamic design method to improve the high-speed aerodynamic performance of an aircraft with low-aspect-ratio tailless configuration. The method can ameliorate the longitudinal moment characteristics of the configuration by designing and collocating the key section airfoils with the constrains of fixed parameters of planform shape and capacity. Firstly, the effect of twisting the wing, fore-loading and aft-reflexing key section airfoils on the high-speed aerodynamic performance of the configuration is evaluated by high-fidelity numerical methods, and quantified by defining trimming efficiency factors. Then, a linear superposition formula is obtained by analyzing the effect rule of trimming efficiency factor, and based on the formula the design and collocation methods of key section airfoils are achieved. According to the methods, a trimmed configuration is obtained. The results of computational fluid dynamics (CFD) and wind tunnel tests show that the trimmed configuration has smaller zero-lift pitching moment and higher available lift-to-drag ratio than the initial configuration at cruise, besides the trimmed configuration achieves the design principle raised for tailless configuration, which can be described as the zero-pitching moment, cruising design lift coefficient, and maximum lift-to-drag ratio are coincident. In addition, at off-design conditions, the trimmed configuration shows favorable drag divergence characteristics, satisfactory aerodynamic characteristics at medium-altitude maneuvering condition, and good stall and pitching-moment performance at low speed state.
Observation, Simulation and Predictability of Fog: Review and Perspectives
Authors: Bergot Thierry , Koracin Darko
Published: February 2021

Fog affects human activities in various ways, but the societal impact of fog has significantly increased during recent decades due to increasing air, marine and road traffic [...]