bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the metabolic regulation mechanism of Yangyin Qingfei decoction plus in severe pneumonia caused by Mycoplasma pneumoniae in mice
Authors: Zhang Tianyu , Zhao Xiyu , Zhang Xining , Liang Xiangyu , Guan Zhenglong , Wang Guanghan , Liu Guanghua , Wu Zhenqi
Published: April 2024

Introduction: With amazing clinical efficacy, Yangyin Qingfei Decoction Plus (YQDP), a well-known and age-old Chinese compound made of ten Chinese botanical drugs, is utilized in clinical settings to treat a range of respiratory conditions. This study examines the impact of Yangyin Qingfei Decoction (YQDP) on lung tissue metabolic products in severe Mycoplasma pneumoniae pneumonia (SMPP) model mice and examines the mechanism of YQDP in treating MP infection using UPLC-MS/MS technology.

Methods: YQDP’s chemical composition was ascertained by the use of Agilent 1260 Ⅱ high-performance liquid chromatography. By using a nasal drip of 1010 CCU/mL MP bacterial solution, an SMPP mouse model was created. The lung index, pathology and ultrastructural observation of lung tissue were utilized to assess the therapeutic effect of YQDP in SMPP mice. Lung tissue metabolites were found in the normal group, model group, and YQDP group using UPLC-MS/MS technology. Using an enzyme-linked immunosorbent test (ELISA), the amount of serum inflammatory factors, such as interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α), was found. Additionally, the protein expression of PI3K, P-PI3K, AKT, P-AKT, NF-κB, and P-NF-κB was found using Western blot.

Results: The contents of chlorogenic acid, paeoniflorin, forsythrin A, forsythrin, and paeonol in YQDP were 3.480 ± 0.051, 3.255 ± 0.040, 3.612 ± 0.017, 1.757 ± 0.031, and 1.080 ± 0.007 mg/g respectively. YQDP can considerably lower the SMPP mice’s lung index (p < 0.05). In the lung tissue of YQDP groups, there has been a decrease (p < 0.05) in the infiltration of inflammatory cells at varying concentrations in the alveoli compared with the model group. A total of 47 distinct metabolites, including choline phosphate, glutamyl lysine, L-tyrosine, 6-thioinosine, Glu Trp, 5-hydroxydecanoate, etc., were linked to the regulation of YQDP, according to metabolomics study. By controlling the metabolism of porphyrins, pyrimidines, cholines, fatty acids, sphingolipids, glycerophospholipids, ferroptosis, steroid hormone biosynthesis, and unsaturated fatty acid biosynthesis, enrichment analysis suggested that YQDP may be used to treat SMPP. YQDP can lower the amount of TNF-α and IL-6 in model group mice as well as downregulate P-PI3K, P-AKT, and P-NF-κB expression (p < 0.05).

Conclusion: A specific intervention effect of YQDP is observed in SMPP model mice. Through the PI3K/Akt/NF-κB signaling pathways, YQDP may have therapeutic benefits by regulating the body’s metabolism of α-Linoleic acid, sphingolipids, glycerophospholipids, arachidonic acid, and the production of unsaturated fatty acids.
Chinese medicine in the treatment of non-alcoholic fatty liver disease based on network pharmacology: a review
Authors: Zheng Shihao , Xue Chengyuan , Li Size , Zao Xiaobin , Li Xiaoke , Liu Qiyao , Cao Xu , Wang Wei , Qi Wenying , Zhang Peng , Ye Yongan
Published: April 2024

Non-alcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by abnormalities in hepatic fat deposition, the incidence of which has been increasing year by year in recent years. It has become the largest chronic liver disease globally and one of the important causes of cirrhosis and even primary liver cancer formation. The pathogenesis of NAFLD has not yet been fully clarified. Modern medicine lacks targeted clinical treatment protocols for NAFLD, and most drugs lack efficacy and have high side effects. In contrast, Traditional Chinese Medicine (TCM) has significant advantages in the treatment and prevention of NAFLD, which have been widely recognized by scholars around the world. In recent years, through the establishment of a “medicine-disease-target-pathway” network relationship, network pharmacology can explore the molecular basis of the role of medicines in disease prevention and treatment from various perspectives, predicting the pharmacological mechanism of the corresponding medicines. This approach is compatible with the holistic view and treatment based on pattern differentiation of TCM and has been widely used in TCM research. In this paper, by searching relevant databases such as PubMed, Web of Science, and Embase, we reviewed and analyzed the relevant signaling pathways and specific mechanisms of action of single Chinese medicine, Chinese medicine combinations, and Chinese patent medicine for the treatment of NAFLD in recent years. These related studies fully demonstrated the therapeutic characteristics of TCM with multi-components, multi-targets, and multi-pathways, which provided strong support for the exact efficacy of TCM exerted in the clinic. In conclusion, we believe that network pharmacology is more in line with the TCM mindset of treating diseases, but with some limitations. In the future, we should eliminate the potential risks of false positives and false negatives, clarify the interconnectivity between components, targets, and diseases, and conduct deeper clinical or experimental studies.
Effectiveness of a sensorimotor exercise program on proprioception, balance, muscle strength, functional mobility and risk of falls in older people
Authors: Freire Ivelize , Seixas Adérito
Published: April 2024

Introduction:

Sensory systems provide the necessary information for a motor response to be provided. In this sense, the objective of this study is to evaluate the effectiveness of a sensorimotor exercise program on proprioceptive acuity, balance, muscle strength, functional mobility and risk of falls in institutionalized elderly.

Methodology:

56 participants (84.6 ± 8.4 years) were randomly distributed between the control (CG, n = 28) and intervention groups (IG, n = 28). The CG performed a protocol based on warm-up, muscle strengthening and warm down and the IG performed the same intervention, with the addition of sensorimotor exercises. Joint Position Sensation (JPS) was evaluated in both limbs at angles of 20° and 45°, balance, functional mobility, fear of falling in the elderly and muscle strength of quadriceps, hamstrings, adductors and abductors in both limbs, before and after the 12 weeks of intervention.

Results:

Both groups showed gains in muscle strength. When analyzing functionality through Timed Up and Go (TUG), before and after for each group separately, both showed a significant difference (CG p = 0.002; IG p < 0.001). For the Short Physical Performance Battery (SPPB) variable, there were significant differences in IG in balance (p < 0.001), gait speed time (s) (p = 0.004) and sit-to-stand (p = 0.002). In JPS, significant differences were recorded for Absolute Error 45° Non-Dominant (p = 0.045) and Relative Error 45° Non-Dominant (p = 0.045) in the CG and Relative Error 45° Non-Dominant for IG (p = 0.018). In the Falls Efficacy Scale International (FES-I) variable there were significant improvements in the CG (p = 0.006) and in the GI (p = 0.002). However, only IG showed significant improvements (p = 0.013) for Activities-Specific Balance Confident (ABC) in a comparison between before and after the 12-week research period. When comparing the differences verified with the intervention between CG and IG, only balance SPPB (p < 0.001) and sit-to-stand SPPB (p = 0.022) showed significant values.

Conclusion:

He effectiveness of sensorimotor exercises provides balance gain in the elderly (p < 0.001) and positively impacts their confidence (p = 0.013) when performing their duties. It is concluded that the protocol presented in its different levels of difficulty is effective and important for the quality of life of the institutionalized sedentary elderly.
Optimal fibre length and maximum isometric force are the most influential parameters when modelling muscular adaptations to unloading using Hill-type muscle models
Authors: Cowburn James , Serrancolí Gil , Colyer Steffi , Cazzola Dario
Published: April 2024

Introduction: Spaceflight is associated with severe muscular adaptations with substantial inter-individual variability. A Hill-type muscle model is a common method to replicate muscle physiology in musculoskeletal simulations, but little is known about how the underlying parameters should be adjusted to model adaptations to unloading. The aim of this study was to determine how Hill-type muscle model parameters should be adjusted to model disuse muscular adaptations.

Methods: Isokinetic dynamometer data were taken from a bed rest campaign and used to perform tracking simulations at two knee extension angular velocities (30°·s−1 and 180°·s−1). The activation and contraction dynamics were solved using an optimal control approach and direct collocation method. A Monte Carlo sampling technique was used to perturb muscle model parameters within physiological boundaries to create a range of theoretical and feasible parameters to model muscle adaptations.

Results: Optimal fibre length could not be shortened by more than 67% and 61% for the knee flexors and non-knee muscles, respectively.

Discussion: The Hill-type muscle model successfully replicated muscular adaptations due to unloading, and recreated salient features of muscle behaviour associated with spaceflight, such as altered force-length behaviour. Future researchers should carefully adjust the optimal fibre lengths of their muscle-models when trying to model adaptations to unloading, particularly muscles that primarily operate on the ascending and descending limbs of the force-length relationship.
Ladder-based resistance training with the progression of training load altered the tibial nerve ultrastructure and muscle fiber area without altering the morphology of the postsynaptic compartment
Authors: Krause Neto Walter , Silva Wellington , Oliveira Tony , Vilas Boas Alan , Ciena Adriano , Caperuto Érico Chagas , Gama Eliane Florencio
Published: April 2024

Scientific evidence regarding the effect of different ladder-based resistance training (LRT) protocols on the morphology of the neuromuscular system is scarce. Therefore, the present study aimed to compare the morphological response induced by different LRT protocols in the ultrastructure of the tibial nerve and morphology of the motor endplate and muscle fibers of the soleus and plantaris muscles of young adult Wistar rats. Rats were divided into groups: sedentary control (control, n = 9), a predetermined number of climbs and progressive submaximal intensity (fixed, n = 9), high-intensity and high-volume pyramidal system with a predetermined number of climbs (Pyramid, n = 9) and lrt with a high-intensity pyramidal system to exhaustion (failure, n = 9). myelinated fibers and myelin sheath thickness were statistically larger in pyramid, fixed, and failure. myelinated axons were statistically larger in pyramid than in control. schwann cell nuclei were statistically larger in pyramid, fixed, and failure. microtubules and neurofilaments were greater in pyramid than in control. morphological analysis of the postsynaptic component of the plantar and soleus muscles did not indicate any significant difference. for plantaris, the type i myofibers were statistically larger in the pyramid and fixed compared to control. the pyramid, fixed, and failure groups for type ii myofibers had larger csa than control. for soleus, the type i myofibers were statistically larger in the pyramid than in control. pyramid and fixed had larger csa for type ii myofibers than control and failure. the pyramid and fixed groups showed greater mass progression delta than the failure. We concluded that the LRT protocols with greater volume and progression of accumulated mass elicit more significant changes in the ultrastructure of the tibial nerve and muscle hypertrophy without endplate changes.
Perspectives on the adaptation of Japanese plum-type cultivars to reduced winter chilling in two regions of Spain
Authors: Guerrero Brenda I. , Fadón Erica , Guerra M. Engracia , Rodrigo Javier
Published: April 2024

Japanese plum, like other temperate fruit tree species, has cultivar-specific temperature requirements during dormancy for proper flowering. Knowing the temperature requirements of this species is of increasing interest due to the great genetic variability that exists among the available Japanese plum-type cultivars, since most of them are interspecific hybrids. The reduction of winter chilling caused by climate change is threatening their cultivation in many regions. In this work, the adaptation perspectives of 21 Japanese plum-type cultivars were analyzed in two of the main plum-growing regions in Spain, Badajoz and Zaragoza, to future climate conditions. Endodormancy release for subsequent estimation of chilling and heat requirements was determined through empirical experiments conducted during dormancy at least over two years. Chill requirements were calculated using three models [chilling hours (CH), chilling units (CU) and chilling portions (CP)] and heat requirements using growing degree hours (GDH). Chilling requirements ranged 277-851 CH, 412-1,030 CU and 26-51 CP, and heat requirements ranged from 4,343 to 9,525 GDH. The potential adaption of the cultivars to future warmer conditions in both regions was assessed using climate projections under two Representative Concentration Pathways (RCP), RCP4.5 (effective reduction of greenhouse gas emissions) and RCP8.5 (continuous increase in greenhouse gas emissions), in two time horizons, from the middle to the end of 21st century, with temperature projections from 15 Global Climate Models. The probability of satisfying the estimated cultivar-specific chilling requirements in Badajoz was lower than in Zaragoza, because of the lower chill availability predicted. In this region, the cultivars analyzed herein may have limited cultivation because the predicted reduction in winter chill may result in the chilling requirements not being successfully fulfilled.
Constraints on novel word learning in heritage speakers
Authors: Ge Yuxin , Rato Anabela , Rebuschat Patrick , Monaghan Padraic
Published: April 2024

Introduction

Recent research on word learning has found that adults can rapidly learn novel words by tracking cross-situational statistics, but learning is greatly influenced by the phonological properties of the words and by the native language of the speakers. Mandarin-native speakers could easily pick up novel words with Mandarin tones after a short exposure, but English-native speakers had specific difficulty with the tonal components. It is, however, unclear how much experience with Mandarin is needed to successfully use the tonal cue in word learning. In this study, we explored this question by focusing on the heritage language population, who typically are exposed to the target language at an early age but then develop and switch to another majority language. Specifically, we investigated whether heritage Mandarin speakers residing in an English-speaking region and speaking English as a dominant language would be able to learn novel Mandarin tonal words from statistical tracking. It helps us understand whether early exposure to the target feature is sufficient to promote the use of that feature in word learning later in life.

Methods

We trained 30 heritage Mandarin speakers with Mandarin pseudowords via a cross-situational statistical word learning task (CSWL).

Results and discussion

Heritage Mandarin speakers were able to learn the pseudowords across multiple situations, but similar-sounding words (i.e., minimal pairs) were more difficult to identify, and words that contrast only in lexical tones (i.e., Mandarin lexical tone) were distinguished at chance level throughout learning. We also collected information about the participants’ heritage language (HL) experience and usage. We did not observe a relationship between HL experience/usage and performance in tonal word learning, suggesting that HL exposure does not necessarily lead to an advantage in learning the target language.
Patient-reported experience measures (PREMs) in outpatient psychiatry – is there an association to perceived discrimination and devaluation?
Authors: Billian Janina , Imfeld Lukas , Roth Carl B. , Moeller Julian , Lang Undine E. , Huber Christian G.
Published: April 2024

Patient-Reported Experience Measures (PREMs) are gaining significance in the field of psychiatry, with patient satisfaction being a key measure. However, it is unclear if patient satisfaction in psychiatry is influenced by variables outside the treatment setting. This brief report thus examines the possible impact of perceived discrimination and devaluation (PDD) on patient satisfaction in the psychiatric outpatient setting. Data from 1,126 individuals who were undergoing or had recently completed treatment at 15 outpatient centers of the Psychiatric University Clinic in Basel, Switzerland, was analyzed. Patient satisfaction was assessed using the Münsterlingen Patient Satisfaction Questionnaire (MüPF), and perceived stigma was measured with the Perceived Discrimination Devaluation Scale. The results revealed a positive small effect size relationship between MüPF and PDD, suggesting that patients who perceived less stigma report higher treatment satisfaction. This relationship may affect most ratings, with the total MüPF score remaining relatively robust against this potential influence. Linear regression analysis indicated that a one-unit increase in PDD score could lead to a maximum change of 1.8 points on the 7-point Likert scale for the MüPF item correlating highest with PDD and 0.4 points on the total MüPF score. These findings highlight the importance of considering perceived stigma when evaluating patient satisfaction with psychiatric outpatient treatment. Future research should investigate associations between stigma, patient satisfaction, treatment outcome, as well as other external factors that may influence patient satisfaction in psychiatric settings.
The prevalence and its associated factors of psychological stress among middle school students in China: pooled evidence from a systematic scoping review
Authors: Hao Li , Mat Ludin Arimi Fitri , Ahmad Mahadir , Meng Xie , Zhong Lei He
Published: April 2024

Psychological issues are of significant concern in present-day society, as poor mental well-being results in depression and suicidal behavior. Understanding the current situation of psychological stress among secondary school students will help policy makers to formulate targeted measures to help them cope with stress, and at the same time evaluate the effectiveness of the existing policies to address the shortcomings and enhance the diversification of interventions. The main purpose of this review was to map the existing evidence on the prevalence and levels of psychological stress among adolescents in China, and to identify the associated risk factors. This review strictly adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). A comprehensive search was performed spanning Web of Science, PubMed, and Scopus databases. Studies involving only humans and full text in English were selected. Selection was limited to samples from mainland China, Hong Kong, Macau, and Taiwan. Variables were extracted, exploring the factors that affected the mental wellness of Chinese middle school students. A final 15 articles and 1 report were included. The findings revealed psychological stress is prevalent among Chinese middle school population, with the degree of prevalence from low to severe stress levels. Three dimensions of psychological stress affecting Chinese secondary school students: school, family and lifestyle. Within the school, the factors included academic stress, peer relationships etc. Family-related factors were comprised of parent-child relationship, parents’ mental health status etc. Lastly, lifestyle-related factors included poor diet, sedentary and inactivity etc. Our findings suggest that policy makers should reduce the excessive emphasis on examination results and focus on the all-round development of students, and that schools should organize a variety of extra-curricular activities to reduce students’ stress. Parents should create a harmonious family atmosphere to minimize conflicts and maintain close communication with teachers.

Systematic Review Registration

OSF; https://doi.org/10.17605/OSF.IO/HEFCP.
Husbands’ knowledge and involvement in sexual and reproductive health rights of women in Bahir Dar City, Northwest Ethiopia: a community-based study
Authors: Jemberie Mekdes Mengesha , Zewdu Meseret , Rade Bayew Kelkay
Published: April 2024

Background

Sexual and reproductive health rights (SRHRs) are integral elements of the rights of everyone to the highest attainable standard of physical and mental health, but they are the most underdeveloped and least understood sphere of rights, especially in Africa, including the country of Ethiopia. The implementation of women’s SRHRs is essential for achieving gender equality and promoting women’s rights. Husbands’ knowledge and involvement play a significant role in improving women’s practice of their SRHRs. However, there is limited information/data about the level of husbands’ knowledge and involvement in Northwest Ethiopia, including Bahir Dar City. Therefore, this study aimed to assess husbands’ knowledge, involvement, and factors influencing their involvement in women’s SRHRs.

Methods

Community-based cross-sectional study design was conducted from March 20 to April 5, 2023, in Bahir Dar City, Northwest Ethiopia, among 391 husbands. Multi-stage sampling and simple random sampling technique were applied to select kebeles and study participants, respectively. Participants were interviewed face-to-face using structured and pretested questionnaire. Binary logistic regression was applied to identify associated factors, and a p-value of <0.05 was a cutoff point to declare statistical significance.

Results

In this study, 50.6% (198/391) of the husbands had good knowledge about their wives’ SRHRs and 44.2% (173/391) (95% CI, 39.3–49.1%) of the husbands were involved when their wives practiced their SRHRs. Access training/education about sexual health [AOR = 5.99; 95% CI (2.7–13.2)], husbands’ advance educational level [AOR = 8.81; 95% CI (2.04–38)], good knowledge about SRHRs [AOR = 7.94; 95% CI (4.3–14.4)], low monthly income (<4,600 birr) [AOR = 9.25; 95% CI (4.2–20.5)], and had open discussion with family members and friends about SRHRs [AOR = 1.92; 95% CI (1.01–3.6)] were found to have significant association with husbands’ involvement.

Conclusion

Husbands’ level of knowledge on SRHRs of women and their involvement remain low. Therefore, responsible concerned bodies need to work on the strategies that help to improve men involvement and knowledge, and tackle the above-mentioned factors influencing their involvement.
Epidemiology, use, and practice of the intraosseous route in an out-of-hospital emergency department: a retrospective cross-sectional study
Authors: Burgos-Esteban Amaya , Quintana-Diaz Manuel , Cordón-Hurtado Valvanera , Giménez-Luzuriaga Marta , Santolalla-Arnedo Iván , de Viñaspre-Hernández Regina Ruiz , Gea-Caballero Vicente , Santos-Sánchez Jose Angel , Navas-Echazarreta Noelia , Rodríguez-Calvo Antonio , Sánchez-Conde Pilar , Juárez-Vela Raúl
Published: April 2024

Introduction

The Spanish Emergency Medical Services, according to the model we know today, were formed during the 80s and 90s of the 20th century. The Health Emergency Service (EMS), 061 La Rioja, began to assist the population of La Rioja in November 1999. An essential part of the mission of the SES is the provision of care and the transfer of critical patients using advanced life support unit (ALSU) techniques. In daily practice, out-of-hospital emergency services are faced with situations in which they must deal with the care of serious or critically ill patients, in which the possibility of being able to channel peripheral vascular access as part of ALSU quickly may be difficult or impossible. In these cases, cannulation of intraosseous (IO) vascular access may be the key to early and adequate care.

Aim

This study aimed to determine the incidence and epidemiology use of IO vascular access in SES 061 La Rioja during the year 2022.

Matherial and methods

We performed observational retrospective cross-sectional studies conducted in 2022. It included a population of 4.364 possible patients as a total of interventions in the community of La Rioja in that year.

Results

A total of 0.66% of patients showed a clinical situation that required the establishment of IO vascular access to enable out-of-hospital stabilization; this objective was achieved in 41.3%. A total of 26.1% of patients who presented with cardiorespiratory arrest (CA) were stabilized, while 100% presented with shock and severe trauma.

Discussion

IO vascular access provides a suitable route for out-of-hospital stabilization of critically ill patients when peripheral vascular access is difficult or impossible.
Understanding sedimentation trends to enhance sustainable reservoir management in the Angereb reservoir, Upper Blue Nile Basin, Ethiopia
Authors: Tessema Yoseph M. , Zimale Fasikaw A. , Kebedew Mebrahtom G.
Published: April 2024

Reservoir sedimentation is a significant issue that shortens reservoir life and impacts operations such as irrigation, hydropower, and drinking water supply because it significantly reduces the reservoir’s initial capacity. Information on changes in the storage capacity is required to reduce the risk of reservoir sustainability. This study was conducted on the Angereb reservoir in the sub-humid region of Ethiopia, in the Upper Blue Nile Basin. Bathymetric surveys and ArcGIS 10.8 were used to estimate the sedimentation and service life of the Angereb reservoir. A Triangulated Irregular Network (TIN) surface was developed from the survey data to estimate the volume of the reservoir. Based on the findings of this bathymetry a total of 2.18 Mm3 of sediment was deposited on the reservoir bed between 2007 to 2022. Storage capacity was decreased by 62.28% in 16 years of operation and caused an average annual volume reduction of 3.9%. The sediment yield (SY) and specific sediment yield (SSY) of the Angereb watershed revealed 192715.5 and 170312.5 m3 year−1 and 3043.2 and 2689.4-ton km−2 y−1 for trap efficiencies of 70.1 and 80% calculated with two methods, respectively. The SSY has increased by about 50.3% to the minimum and 70.1% to the maximum indicating more degradation of the watershed in sixteen years. The gross storage capacity loss of the reservoir was about 75% which was 5.3 Mm3 in 1997 and reduced to 1.32 Mm3 in 2022 with an annual reduction of 2.9% in storage capacity. The reservoir will be filled up by sediment in 7 years if the incoming sediment remains the same in the future. These findings will serve as a call for action in the watershed and a foundation for managing the accumulated sedimentation in the Angereb reservoir and for developing sediment control and management strategies for similar reservoirs.
The 100 most cited articles in artificial intelligence related to orthopedics
Authors: Turgut Necmettin , Beyaz Salih
Published: April 2024

Background

This bibliometric study aimed to identify and analyze the top 100 articles related to artificial intelligence in the field of orthopedics.

Methods

The articles were assessed based on their number of citations, publication years, countries, journals, authors, affiliations, and funding agencies. Additionally, they were analyzed in terms of their themes and objectives. Keyword co-occurrence, co-citation of authors, and co-citation of references analyses were conducted using VOSviewer (version 1.6.19).

Results

The number of citations of these articles ranged from 32 to 272, with six papers having more than 200 citations The years of 2019 (n: 37) and 2020 (n: 19) together constituted 56% of the list. The USA was the leading contributor country to this field (n: 61). The most frequently used keywords were “machine learning” (n: 26), “classification” (n: 18), “deep learning” (n: 16), “artificial intelligence” (n: 14), respectively. The most common themes were decision support (n: 25), fracture detection (n: 24), and osteoarthrtitis staging (n: 21). The majority of the studies were diagnostic in nature (n: 85), with only two articles focused on treatment.

Conclusions

This study provides valuable insights and presents the historical perspective of scientific development on artificial intelligence in the field of orthopedics. The literature in this field is expanding rapidly. Currently, research is generally done for diagnostic purposes and predominantly focused on decision support systems, fracture detection, and osteoarthritis classification.
Liver transcriptome and physiological analyses preliminarily revealed the adaptation mechanisms of Amur grayling (Thymallus arcticus grubei, Dybowski, 1869) fry for dietary lipid nutrition
Authors: Fan Ze , Ma Kai , Wang Yan , Wang Liansheng , Zhang Yongquan , Li Chenhui , Li Jiaxin , Wu Di , Li Jinnan , Li Zhengwei
Published: April 2024

The Amur grayling (Thymallus arcticusgrubei Dybowski, 1869), a species of potentially economic and research value, is renowned for its tender meat, exquisite flavor, and high nutritional contents. This study was conducted to investigate the physiological adaptation mechanisms to dietary lipids in Amur grayling fry (with average initial weight 4.64±0.03 g). This study involved a 56-day feeding trial with diets containing varying lipid levels (9.07%, 12.17%, 15.26%, 18.09%, 21.16%, and 24.07%, designated as GL1 through GL6, respectively) to explore the impact of dietary lipids on growth performance, intestinal digestion, liver antioxidative function, and transcriptomic profiles. Results showed that The group receiving 18% dietary lipid exhibited a markedly higher weight gain rate (WGR) and specific growth rate compared to other groups, alongside a reduced feed conversion ratio (FCR), except in comparison to the 15% lipid group. Activities of lipase in pancreatic secretion and amylase in stomach mucosa peaked in the 18% lipid treatment group, indicating enhanced digestive efficiency. The liver of fish in this group also showed increased activities of antioxidative enzymes and higher levels of glutathione and total antioxidative capacity, along with reduced malondialdehyde content compared to the 9% and 24% lipid treatments. Additionally, serum high-density lipoprotein cholesterol levels were highest in the 18% group. Transcriptomic analysis revealed four significant metabolic pathways affected: Cholesterol metabolism, Fat digestion and absorption, PPAR signaling, and Fatty acid degradation, involving key genes such as Lipase, Lipoprotein lipase, Fatty acid-binding protein, and Carnitine palmitoyltransferase I. These findings suggest that the liver of Amur grayling employs adaptive mechanisms to manage excessive dietary lipids. Quadratic regression analysis determined the optimal dietary lipid levels to be 16.62% and 16.52%, based on WGR and FCR, respectively. The optimal dietary lipid level for juvenile Amur grayling appears to be around 18%, as evidenced by improved growth performance, digestive function, balanced serum lipid profile, and enhanced liver antioxidative capacity. Exceeding this lipid threshold triggers both adaptive and potentially detrimental liver responses.
Study on the Penetration Performance of a Double-Angle Linear Shaped Charge: Performance Improvement and Miniaturization
Authors: Park Jongmin , Kwon Sejin
Published: April 2024

This study was conducted on a linear shaped charge with a double-angle liner. The double-angle liner has a large inner apex angle and a small outer liner angle. Experiments and numerical analysis were performed in a penetration performance study, and it was confirmed that the experimental results and numerical analysis results matched well. As a result of the numerical analysis, at the standoff distance of 1.5 CD, the penetration performance of the double-angle linear shaped charge was improved by 14.5% compared to the conventional linear shaped charge, and at the standoff distance of 2.5 CD, the penetration performance was improved by 12.5%. For miniaturization, numerical analysis was performed by reducing the height of the explosive and the standoff distance. As a result of the numerical analysis, the penetration performance of the double-angle linear shaped charge was improved by 14.6% compared to the conventional linear shaped charge. Double-angle liners are effective in improving the penetration performance of linear shaped charges.
The Use of a New Ionic Derivative of Salicylic Acid in Sugar Beet Cultivation
Authors: Kukawka Rafal , Spychalski Maciej , Grzempa Bartosz , Smiglak Marcin , Górski Dariusz , Gaj Renata , Kiniec Agnieszka
Published: April 2024

The need for sustainable development in the context of pesticide use has been recognized by the European Union. The “Farm to Fork Strategy” indicates a goal of 50% reduction in pesticide use by 2030. To address this challenge, we used the concept of ionic liquids to modify known resistance inducers, i.e., a group of substances whose action is indicated as an alternative to fungicides. A new, patented substance developed by us, which is a choline 3,5-dichlorosalicylate, has been tested in the context of its use in sugar beet cultivation with the aim of controlling Cercospora leaf spot (CLS). The results suggest that the use of this substance in combination with one fungicide treatment reduces disease infection and produces yields very similar to the use of a standard protection program assuming the use of two fungicides. Such results provide the basis for further development of 3,5-dichlorosalicylate in terms of its use in agriculture. Thanks to its use, it was possible to resign from one fungicide treatment, while maintaining protection against CLS and yields at the same level as for the full fungicide protection program. Such an approach is in line with European Union policies.
The Influence of Different, Long-Term Fertilizations on the Chemical and Spectroscopic Properties of Soil Organic Matter
Authors: Weber Jerzy , Mielnik Lilla , Leinweber Peter , Hewelke Edyta , Kocowicz Andrzej , Jamroz Elżbieta , Podlasiński Marek
Published: April 2024

Currently, revealing soil management strategies that store the maximum atmospheric CO2 in the soil is a major issue. This is best explored by investigating long-term experiments, like the Skierniewice (Poland) field trial, established in 1921 on sandy loam Luvisol. In this trial, the variants analyzed included control (CON), manure (MAN), legumes (LEG), and manure + legumes (MAN + LEG). Soil samples from the A horizon were analyzed for total organic carbon (TOC), carbon content of humic acids (HA), fulvic acids (FA), and humin (HUM), as well as for spectroscopic properties of bulk soil and isolated HUM. Compared to the control, all other treatments caused an increase in TOC, while the application of manure resulted in an increase in the amount of HUM. Legume application caused an increase in UV-Vis absorbance and fluorescence emission. Thermochemolysis and gas chromatography/mass spectrometry showed that HUM was enriched in carbohydrates in almost all pairs of soil and HUM. Compared to the CON, the largest proportion of carbohydrate in HUM was found in MAN + LEG. Different long-term soil management strategies not only altered TOC, but also, surprisingly, the chemical composition of HUM, which is considered to be particularly stable and a long-term sink of atmospheric carbon.
Brassinosteroids: Relevant Evidence Related to Mitigation of Abiotic and Biotic Stresses in Plants
Authors: Silva Aminthia Pombo Sudré da , Alencar Antônio André da Silva , Sudré Cláudia Pombo , Araújo Maria do Socorro Bezerra de , Lobato Allan Klynger da Silva
Published: April 2024

Extreme events of climate change are increasing, such as droughts and heat waves, causing limitations on growth and yield in relevant food crops, as well as threatening global food security. Brassinosteroids (BRs) are natural or synthetic steroids with significant properties that promote plant growth and development. In the current world scenario, research and solutions that can improve plant tolerance to climate change are strategic to ensure food security. The distinctiveness and novelty of this review lie in its comprehensive and detailed approach to the role of BRs in plants under biotic and abiotic stresses. We consolidate information on the action mechanisms on specific organs, providing detailed experimental conclusions of these plant growth regulators, including also commercial products and concentrations tested aiming to mitigate the adverse effects of the stresses. This practical approach highlights the potential of BRs in agriculture and plant protection against stresses. Additionally, our review presents results with plant models and essential food crops, focusing on multidisciplinary approaches and using physiological, biochemical, nutritional, anatomical and agronomic tools to explain the mechanisms of action of brassinosteroids in plants exposed to abiotic and biotic stresses.
External Hoof Measurements of Untrimmed and Unshod Mules in Northern Thailand
Authors: Phannithi Thawijit , Laikul Aree , Pathomsakulwong Watcharapol , Rungsri Porrakote , Apichaimongkonkun Tawanhathai , Watchrarat Krisana , Cherdchutham Worakij
Published: April 2024

External hoof characteristics, balance, and conformation have been extensively studied in horses; however, mules remain understudied in these aspects. This study evaluated the size, shape, and symmetry of untrimmed and unshod forelimb hooves, compared the symmetry between forelimb hooves and stratified external forelimb hoof measurements based on the body condition score of mules raised in the foothill plains of northern Thailand. The forelimb hooves of 38 mules were photographed and 33 parameters, including angular and linear measurements, were analyzed. A multivariate analysis was used to explore the influence of sex, age, and body condition scores (BCS) on angular, linear, and area parameters. Additionally, one-way ANOVA followed by Tukey’s test was used to compare these parameters across different BCS groups. Despite the absence of shoeing and trimming, these mules exhibited optimal left–right forelimb hoof symmetry, with no significant (p < 0.05) differences in: outer wall length and inner wall length (OWL–IWL: Left 0.11 ± 0.66 cm; Right −0.12 ± 0.43 cm); sole length and sole width (SLS–SW: Left 1.65 ± 0.76 cm; Right 1.46 ± 0.89 cm); dorsal hoof wall length and heel length (DHWL–HL: Left 4.00 ± 0.80 cm; Right 3.81 ± 0.72 cm); and frog length and frog width (FL–FW: Left 3.88 ± 1.13 cm; Right 3.82 ± 0.18 cm). However, significant (p < 0.05) differences were observed within each body condition score group for forelimb hoof measurements for DHWL, IWL, heel separation (HS), heel bulb distance (HBD), SW, FW, and FL, while sex and age had no significant differences across the study variables. These findings provide valuable insights into mule welfare and management, contributing to understanding of the interplay between overall health and hoof conformation in the study area.
Herpesvirus and Subsequent Usutu Virus Infection in a Great Grey Owl (Strix nebulosa) at the Ljubljana Zoo, Slovenia
Authors: Žlabravec Zoran , Kvapil Pavel , Slavec Brigita , Zorman Rojs Olga , Švara Tanja , Račnik Jožko
Published: April 2024

Herpesvirus (HV) has been known to cause disease in owls, with various clinical signs and outcomes for the last several decades. The HV DNA polymerase gene was detected in oropharyngeal and cloacal swabs of a male great grey owl (Strix nebulosa) in a zoological collection in Ljubljana, Slovenia. In the following 4 months, despite continuous HV detection in swabs, no clinical signs with a clear link to HV disease were observed. Hepatoprotective and immunostimulant therapies applied during this period did not prevent HV shedding. Therefore, peroral antiviral therapy with acyclovir (150 mg/kg q24 h for seven days) was performed, and the owl tested negative at the next sampling and remained negative for the next 8 months. After that, the owl again tested positive for HV presence, and the same protocol with antiviral therapy was performed. After 3 weeks with a negative test for HV presence, without any clinical signs of illness, the owl suddenly died because of Usutu virus (USUV) infection. Among all the owls at the zoo, interestingly, only the HV-positive great grey owl died because of USUV infection. The USUV sequence detected and obtained in this study clusters together with other Europe 2 sequences detected in neighboring countries. Our study shows the potential of acyclovir therapy in the prevention of herpesvirus shedding and, moreover, lowering the possibility for spreading HV to other owls and birds. To the best of our knowledge, this is the first report of HV presence and USUV infection in a great grey owl in Slovenia.
Whole-Genome Sequencing Analyses Reveal the Evolution Mechanisms of Typical Biological Features of Decapterus maruadsi
Authors: Deng Wen-Jian , Li Qian-Qian , Shuai Hao-Nan , Wu Ren-Xie , Niu Su-Fang , Wang Qing-Hua , Miao Ben-Ben
Published: April 2024

Decapterus maruadsi is a typical representative of small pelagic fish characterized by fast growth rate, small body size, and high fecundity. It is a high-quality marine commercial fish with high nutritional value. However, the underlying genetics and genomics research focused on D. maruadsi is not comprehensive. Herein, a high-quality chromosome-level genome of a male D. maruadsi was assembled. The assembled genome length was 716.13 Mb with contig N50 of 19.70 Mb. Notably, we successfully anchored 95.73% contig sequences into 23 chromosomes with a total length of 685.54 Mb and a scaffold N50 of 30.77 Mb. A total of 22,716 protein-coding genes, 274.90 Mb repeat sequences, and 10,060 ncRNAs were predicted, among which 22,037 (97%) genes were successfully functionally annotated. The comparative genome analysis identified 459 unique, 73 expanded, and 52 contracted gene families. Moreover, 2804 genes were identified as candidates for positive selection, of which some that were related to the growth and development of bone, muscle, cardioid, and ovaries, such as some members of the TGF-β superfamily, were likely involved in the evolution of typical biological features in D. maruadsi. The study provides an accurate and complete chromosome-level reference genome for further genetic conservation, genomic-assisted breeding, and adaptive evolution research for D. maruadsi.
Study of Salmonella spp. from Cage Papers Belonging to Pet Birds in an Argentinean Canary Breeder Championship
Authors: Bueno Dante J. , Rodríguez Francisco I. , Machado Luciana C. , Soria Mario A. , Procura Francisco , Gómez Silvana C. , Hoffmann Teresa M. , Alcain Andrea , Caffer María I. , Latorre Juan D. , Quintar Javier O.
Published: April 2024

Birds, including canaries and other birds, have become increasingly popular as pets. Bird fairs, where breeders gather and show their production in a championship setting, present a setting for possible Salmonella spp. contamination and transmission. Therefore, this study estimated the rate of Salmonella spp. isolation from cage papers, located in the bottom of cages of exotic pet birds, including canaries. Collected Salmonella isolates were used to determine the antimicrobial resistance profile to 52 antibiotics and 17 commercial disinfectants, based on pure or a mixture of acids, alcohols, aldehydes, alkalis, halogens, peroxygen, and quaternary ammonium compounds. The samples consisted of 774 cage papers taken in the 2015 Argentinean canary breeder championship, pooling three cage papers into one sterile sampling bag. Only one pool of the cage papers was positive for Salmonella spp. (0.4%), which belonged to the sample from three frill canary cages. Two strains of Salmonella serotype Glostrup were isolated, which were only resistant to sulfonamides and erythromycin and sensitive to alkali-based product PL301 AS. Although the rate of Salmonella spp. isolation from cage papers in an Argentinean canary breeder championship is low, it should not be discounted because Salmonella ser. Glostrup can be a source of human Salmonella outbreaks and they show high resistance to disinfecting products.
Some Early Studies of Isotropic Turbulence: A Review
Authors: Shi John Z.
Published: April 2024

A re-examination of some early classic turbulence literature, mainly of isotropic turbulence, is given in this selective review. Some early studies, including original concepts and points, are reviewed or highlighted. Two earliest studies and six major original concepts are found: (i) Lord Kelvin’s pioneering elementary studies of homogeneous, isotropic turbulence; (ii) Kelvin’s early introduction of Fourier Principles into turbulence studies; (iii) the Kelvin elementary concept of the direct energy cascade; (iv) the Kelvin early concept of the symmetry of turbulence; (v) the Taylor concept of the coefficient of eddy viscosity; (vi) the Taylor concept of the ‘age’ of the eddy; (vii) the Taylor–Fage–Townend concept of small eddies or microturbulence or small scale turbulence; and (viii) the Obukhov concept of a function of the inner Reynolds number (i.e., Re dependent coefficient) in both the balance equation and the energy distribution equation (the two-thirds law). Both Kelvin and Taylor should be regarded as the co-founders of the statistical theory of homogeneous, isotropic turbulence. The notion, ‘the Maxwell–Reynolds decomposition of turbulent flow velocity’, should be used. The Kolmogorov–Obukhov scaling laws are reviewed in detail. Heisenberg’s inverse seventh power spectrum is briefly reviewed. The implications or significances of these early studies, original concepts and points are briefly discussed, with special reference to their possible links with modern approaches and theories.
Implementing an Early Detection Program for Autism Spectrum Disorders in the Polish Primary Healthcare Setting—Possible Obstacles and Experiences from Online ASD Screening
Authors: Sobieski Mateusz , Grata-Borkowska Urszula , Bujnowska-Fedak Maria Magdalena
Published: April 2024

A screening questionnaire for autism symptoms is not yet available in Poland, and there are no recommendations regarding screening for developmental disorders in Polish primary healthcare. The aim of this study was to assess the opinions of parents and physicians on the legitimacy and necessity of screening for autism spectrum disorders, potential barriers to the implementation of the screening program, and the evaluation and presentation of the process of online ASD screening, which was part of the validation program for the Polish version of one of the screening tools. This study involved 418 parents whose children were screened online and 95 primary care physicians who expressed their opinions in prepared surveys. The results indicate that both parents and doctors perceive the need to screen children for ASD in the general population without a clear preference as to the screening method (online or in person). Moreover, online screening is considered by respondents as a satisfactory diagnostic method. Therefore, online screening may prove to be at least a partial method of solving numerous obstacles indicated by participants’ systemic difficulties including time constraints, the lack of experienced specialists in the field of developmental disorders and organizational difficulties of healthcare systems.