bluuebunny/crossref_metadata_2025 huggingface dataset:
Three-column Subdivision for Isolated Posterolateral Tibial Plateau Fractures and Perspective Surgical Approachs
Authors: Sim Jae Ang , Park Ji Hyeon , Na Young Gon , Lee Byung Hoon
Published: July 2020

Background: Management of the tibial plateau fracture involving posterolateral compartment is technically challenging. This study aimed to introduce a computed tomography-based classification of the posterolateral compartment of tibial plateaus based on the fibula and to propose the individualized surgical approaches for each zone; and 2) to determine the surgical approach based on the classification, would achieve a safe and effective reduction and can improve postoperative clinical outcomes.Methods: Eighteen cases of tibia plateau fracture involved the isolated posterolateral compartment in a single institution were retrospectively analyzed. The posterolateral compartment of tibial plateau was segmented into three zones based on the fibular position and individualized surgical approach was proposed for each zone. In anterior Zone I, surgical treatment was performed using an extended anterolateral approach and patient was placed in the supine position; In middle Zone II, using the transfibular approach in the supine position; In posterior Zone III, using the posteromedial approach in prone. Articular reduction (intra-articular step off in CT images) and mechanical medial proximal tibial angle (mMPTA) in simple radiographs taken in the immediate postoperative period and clinical outcomes of the Lysholm knee scoring scale and range of motion were evaluated at postoperative 1-year follow-up,Results: In all cases, anatomical articular reduction (intra-articular step off < 2mm) was achieved, maintained for the follow-up period. The average mMPTA was increased from 87.6º before surgery to 88.2º in immediate postoperative period (p = 0.060), and maintained for the follow-up period (mean 89.9º at one-year follow-up). At the 1-year follow-up, the knee range of motion averaged 140 degrees and the Lysholm knee function scored 95.0 points.Conclusion: An individualized surgical approach and fixation according to three-zone subdivision for isolated posterolateral tibial plateau fractures provided an effective and safe method to treat posterolateral tibial plateau fractures. Level of evidence: Therapeutic study, IV
Experience of N-acetylcysteine airway management in the successful treatment of one case of critical condition with COVID-19
Authors: Liu Yan , Luo Guoshi , Qian Xin , Wu Chenglin , Tang Yijun , lu kun , Chen Biyu , Leung Elaine Lai-Han , Wang Meifang
Published: July 2020

Object: To report the successful diagnosis and treatment of a patient with critical condition of novel coronavirus pneumonia (COVID-19) and to summarize its clinical features and airway management experience in successful treatment.Methods: Retrospectively analyzed the successful management of one case of COVID-19 with critical condition combined respiratory failure and discussed the clinical characteristics and airway management of the patient in conjunction with a review of the latest literature.Results: A patient with an anastomotic fistula after radical treatment of esophageal cancer and right-side encapsulated pyopneumothorax was admitted with cough and dyspnea and was diagnosed with novel coronavirus pneumonia and malnutrition by pharyngeal swab nucleic acid test in combination with chest CT. The patient was treated with antibiotics, antiviral and antibacterial medications, respiratory support, expectorant nebulization, and nutritional support, expressed progressive deterioration. Endotracheal intubation and mechanical ventilation were performed since the onset of the type Ⅱ respiratory failure on the 13th day of admission. The patient had persistent refractory hypercapnia after mechanical ventilation. Based on the treatment mentioned above, combined with repeated bronchoalveolar lavage by using N-acetylcysteine ​​(NAC) inhalation solution, the patient's refractory hypercapnia was gradually improved. It was cured and discharged after being given the mechanical ventilation for 26 days as well as 46 days of hospitalization, currently is surviving well.Conclusion: Patients with severe conditions of novel coronavirus pneumonia often encounter bacterial infection in their later illness-stages. They may suffer respiratory failure and refractory hypercapnia that is difficult to improve due to excessive mucus secretion leading to small airway obstruction. In addition to the use of reasonable antibiotics and symptomatic respiratory support and other treatment, timely artificial airway and repeated bronchoalveolar NAC inhalation solution lavage, expectorant and other airway management are essential for such patients.
Study of Structural and Magnetic Properties, Trivalent Cation Substitution of Cobalt base Spinel Ferrites CoCr0.04LaxFe1.96-xO4 (0 ≤ x ≤ 0.12)
Authors: MUSTAFA G. , FAROOQ Z. , AHMAD M.R. , ANWAR H. , AKHTAR H. , YASEEN M. , QAYYUM M. A. , MAHMOOD MIAN H.R.
Published: July 2020

In this research work a series of polycrystalline trivalent cation (Cr3+, La3+) substituted cobalt ferrite with general formula CoCr0.04LaxFe1.96-xO4 where 0.00 ≤ x ≤ 0.12 (in steps of 0.02) was synthesized using co-precipitation method and were sintered at 900°C for 6 hours. The phase identification and confirmation of the structure were confirmed employing the X-ray diffraction (XRD) and FTIR techniques. The size of crystallite was found on average, in the range of 53-106 nano-meters. FTIR results confirmed spinel ferrites structure. Moreover the morphological studies were observed through SEM. The elemental analysis of the samples was done by EDX while, I-V characterization of the represented sample was recorded using two probe method. The measured electrical resistivity of materials increased as dopant content increased. The magnetic behavior of the materials was studied using vibrating sample magnetometer (VSM). The saturation magnetization values and magnetic coercivity values decreased with the increase of La3+ concentration. The saturation magnetization (Ms) of the ferrite materials decreased with the reduction of size.
Effectiveness of Theory of Planned Behavior-Based Educational Intervention on Newborn Care in Pregnant Mothers: A quasi-experimental study
Authors: Navabi Azamalsadat , shamsi mohsen , Khorsandi Mahboobeh , Zamanian Maryam
Published: July 2020

Background and Purpose: The neonatal period is one of the most sensitive stages of life, requiring proper understanding and attentive care. Accordingly, this study aimed to investigate the effectiveness of theory of planned behavior-based educational intervention on newborn care in pregnant mothers. Methodology: This study was conducted as a quasi-experimental research on 100 pregnant mothers who were randomly assigned to two groups of intervention (N=50) and control (N=50). The data were collected using a theory of planned behavior-based questionnaire. Considering the effective constructs of this theory, the educational intervention was performed in the form of four 60-minute education sessions in one month in the intervention group. One month after delivery, the data were recollected from both groups and analyzed using statistical tests.Findings: The mean and standard deviation of the mothers’ age in both intervention and control groups were 26.14±4.7 and 26.10±5.19, respectively (p=0.48). Before the educational intervention, the behavioral intention of the intervention group was 3.33 (out of 5), which significantly increased to 4.54 after the educational intervention (p<0.001). In addition, the performance of mothers regarding neonatal care was significantly enhanced from 2.86 to 4.64 one month after the delivery (p=0.001). Conclusion: The theory of planned behavior-based educational intervention, along with active follow-ups, led to better and more principled behavioral intention and care provided by pregnant mothers to their newborn infants. Therefore, providing theory of planned behavior-based education in other healthcare centers is recommended.
A Positive Feedback Regulation Involving PVT1 and HER2/hER3 Promotes Progression of High-grade Serous Ovarian Cancer
Authors: wang Wenxiang , Fan Wenqiang , Gao Yuxia , Liu Yu , Li Li , Yi Lisha , He Qian , Hai Jing , Dong Xuecai , Chen Caixia
Published: July 2020

Background

In recent years, long noncoding RNAs (lncRNAs) have been reported frequently to play important roles in specific cancers, including high-grade serous ovarian cancer (HGSOC). PVT1 is an important oncogenic lncRNA highly expressed in various cancers. However, little is known about the role of PVT1 in HGSOC and underlying mechanisms.
Methods

The expression levels of PVT1 and HER2/3 in HGSOC tissue and adjacent normal tissue were determined by qRT-PCR. MTT and transwell assays were used to identify the effects of PVT1 cell proliferation and migration respectively. Dual-luciferase reporter assay and RIP experiment were carried out to verify target genes of PVT1. ChIP experiment was used to identify that HER2 was transcription factor of PVT1.
Results

Our results showed that PVT1 expression was up-regulation in human HGSOC specimens and promoted ovarian cancer cell proliferation and migration. We further validated that HER2 was a direct transcription factor for facilitating PVT1 expression. In return, PVT1 enhanced HER2 transcript stability. Moreover, bioinformatics analysis and dual luciferase reporter assay results uncovered that PVT1 functioned as a competing endogenous RNA (ceRNA) for miR-1301-3p to promote cell proliferation and migration by increasing HER3 expression.
Conclusions

Taken together, our results showed that PVT1, controlled by HER2, elevated HER2 and HER3 expression to promote HGSOC progression. Thus, PVT1 can be regarded as a vital diagnostic biomarker for HGSOC and a potential novel therapeutic target.
Ethical Decision-Making of Health Professionals Caring for HIV-Infected People in Hunan, China: A Qualitative Study
Authors: Huang Hangyu , Koniak-Griffin Deborah , Wang Honghong , Yang Min
Published: July 2020

Background: Numerous ethical issues surged the moment AIDS was discovered. As advocates of HIV-infected people, health professionals encounter many ethical dilemmas in clinical practice. However, it remains unclear how health professionals solve these issues. To explore the ethical decision-making experiences of health professionals who care for HIV-infected people in Hunan Province, China, and to discuss the strategies for enhancing ethical decision-making competence in AIDS care. Methods: This descriptive qualitative research adopted semi-structured, in-depth interview and thematic analysis. Participants were recruited by purposive sampling. In total, twenty-two participants completed the interview. Results: Three themes emerged from data analyses of the interviews: (1) common ethical dilemma experienced by health professionals, (2) factors influencing ethical judgment, (3) ethical motivations. Conclusion: About two thirds of informants failed to recall ethical dilemmas experienced in their clinical practice. Emotions, gender, occupation, and difficulty balancing different roles may influence the ethical judgments of health professionals. In the ethical decision-making process, most informants took other people’s interests into consideration and conformed to law and professional codes of conduct. However, negative attitudes towards HIV-infected people still exist among a few professionals. Furthermore, some informants showed a misunderstanding of ethical principles. And the fear of medical disputes (conflicts with families and others) was experienced by many informants, influencing their ethical behaviors. Hence, more efforts are needed to eliminate negative attitudes towards HIV-infected patients. This study underscores the importance of continuing ethical education for HIV/AIDS-related professionals to enhance their ethical decision-making competence. Moreover, sound governmental laws may promote ethical behavior in AIDS care.
Screening and identifcation of differentially expressed microRNAs in diffuse large B-cell lymphoma based on microRNA microarray
Authors: Gao Hai-Xia , Li Xinxia
Published: July 2020

We aimed to screen and identify differentially expressed microRNAs (miRNAs) between diffuse large B-cell lymphoma (DLBCL) and control (lymph node reactive hyperplasia, LRH) groups, investigate whether miRNAs associated with DLBCL and could serve as potential therapeutic targets. Five DLBCL experimental samples and five control samples were obtained from fresh tissues. The exclusion criteria were expressed as follows: patients with other lymphoid diseases and patients undergoing chemical treatment. Firstly, fresh samples were analyzed using microRNA microarray to identify differentially expressed miRNAs. Next, three databases (Targetscan, microRNAorg, PITA) were used to intersection predict the potential target gene of the 204 differential miRNAs and performed Venn diagram of the results. Then, target genes of differential miRNAs were performed by Gene ontology (GO) and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway analysis. Finally, to validate the microRNA microarray data, we performed Real-time reverse transcription PCR (qRT-PCR) for eight differentially expressed miRNAs (miR-193a-3p, miR-19a-3p, miR-19b-3p, miR-370-3p, miR-1275, miR-490-5p, miR-630 and miR-665) using DLBCL and LRH fresh samples. Two hundred and four miRNAs showed differential expression, including 105 down-regulated and 54 up-regulated miRNAs. The cut-off criterion was set as P value ≤0.05 and fold change (FC) ≥2. A total of 7522 potential target genes for the 204 miRNAs were predicted. Potential target genes were enriched in pathway in cancers, MAPK signal pathway, regulation of actin cytoskeleton, focal adhesion, endocytosis, Wnt signal pathway, axon guidance, calcium signal pathway, and PI3K/AKT signal pathway. Eight miRNAs validation by qRT-PCR, four miRNAs (miR-19b-3p, miR-193a-3p, miR-370-3p and miR-490-5p) were low expression in DLBCL (P< 0.05). The high expression of miR-630 in DLBCL was statistically significant (P< 0.05). Our results of identification of differentially expressed miRNAs, predicted the target gene of the differential miRNAs, enrichment of target gene biological functions and signaling pathway, will provide basis for researchers in identifying the pathogenesis of DLBCL, could serve as reliable biomarkers for precise diagnosis and as therapeutic target for improvement of therapeutic efficacy in DLBCL in the future.
Accuracy of Ultrasound Estimation of Fetal Weight at Three Teaching Hospitals in Addis Ababa
Authors: Ibrahim Alexander Mohamed , Weldetensaye Eskinder Kebede
Published: July 2020

BackgroundUltrasound examination and measurement of fetal weight have become an integral part of modern obstetric care. Knowing the accuracy of the ultrasound estimation of fetal weight (EFW) and what factors affect it helps make a more informed decision. Fetal weight estimation is thought to help predict fetal survival and make management decisions in the low birth weight group and in managing the delivery of the large baby, where complications may occur.ObjectiveTo assess the accuracy of ultrasound estimation of fetal weight.MethodsThe study design was a facility-based prospective cross-sectional study comparing sonographic EFW with actual birth weight, involving 384 women admitted to the study hospitals. And assessed 12 factors that might affect accuracy (place of delivery/scan, level of the operator, time of scan (working vs. non-working hours), fetal presentation, liquor volume, placental location, days passed between scan and delivery, time of the scan to labor (antepartum vs. intrapartum), fetal sex, maternal BMI, gestational age, and fetal weight. Data were collected using structured questionnaires, developed based on study variables and available literature, and analyzed using SPSS software.ResultThe EFW that is within 10% of the actual birth weight is 62.4%. The overall mean error of EFW was 9.1%, with an SD of ± 7.1%. Of the 12 factors assessed, it was found that the place of delivery/scan (p = 0.017) and the level of resident experience (p = 0.47) significantly affected the accuracy. When R4 was compared to R1’s OR = 4.656(95%CI, [1.111–19.506], p = 0.035) and R2’s OR = 2.112(95%CI,[1.062–4.201],p = 0.033) but not significant when compared to R3’s OR = 1.227(95%CI,[0.563–1.191],p = 0.607). A paired sample t-test was applied (P = 0.14).Conclusion and recommendationThe EFW of R4’s was significantly different from that of R1’s and R2’s, but not R3’s we recommend that all ultrasound scans whose weight estimation can affect the management plan of the patient to be done by the senior residents rather than the junior residents i.e. the R3’s and R4’s.
Subcellular Localization of Cerebellar Degeneration-Related Protein 1
Authors: Solheim Eirik Tveit , Kråkenes Torbjørn , Totland Cecilie , Vedeler Christian Alexander
Published: July 2020

Background: Cerebellar degeneration-related protein 1 (CDR1) is associated with paraneoplastic cerebellar degeneration – an autoimmunedisease characterized by neurodegeneration in the cerebellum. Paraneoplastic syndromes are believed to be triggered by ectopic tumour expression of neuronal proteins. The CDR1 protein is expressed by neurons in the cerebellar cortex and in certain cancers. In this study, we characterized the subcellular localization of CDR1 to help delineate potential functions in cancer cells and neurons. Methods: Ovarian cancer cells, cerebellar sections, and Purkinje cells in culture were immunostained and imaged by super-resolution stimulated emission depletion microscopy and potential interaction partners were investigated using a proximity ligation assay. Results: CDR1 was localized to the mitochondria in cancer cells and was colocalized with proteins of the outer mitochondrial membrane. Using a proximity ligation assay, we discovered that CDR1 interacts with mitofusin 1 – a transmembrane GTPase involved in mitochondrial fusion – in both cancer cells and rat cerebellum. CDR1 also colocalized with intermediate filaments, including vimentin, in cancer cells and with neurofilaments in human cerebellum. Conclusion: The dual localization to mitochondria and intermediate filaments as well as colocalization with key proteins involved in mitochondrial dynamics suggest that CDR1 is involved in the subcellular distribution of mitochondria in cells.
Genetic characteristics and prognosis of m6A RNAmethylation regulator in acute myeloid leukemia
Authors: Xu Jiasheng , Liao Kaili , Fu Zhonghua , Xiong Zhenfang
Published: July 2020

Background: To identify the genetic characteristics of m6A RNA methylation regulators in AML and explore their potential value as prognostic markers.Methods: RNA-seq transcriptome data and clinical survival data of acute myeloid leukemia (AML) were downloaded from ICGC and TCGA, gene annotation files were downloaded from GENECODE (1). 13 widely reported m6A RNA alphas were obtained from the literature. The expression of m6A RNA methylation regulators were collected and analized using gene annotation files. The samples were subjected to consistent clustering to obtain two subgroups RM1 and RM2, and the pathological characteristics and survival between the two subgroups were analyzed. Comparative analysis and functional analysis of m6A RNA methylation regulators between subgroups were completed. The STRING database analyzed the interactions between m6A RNA methylation regulators, and Spearman analyzed the correlation of expression of m6A RNA methylation regulators. COX regression analysis and risk scores were used to predict prognosis and pathological characteristics, and risk scores calculated using features were used to predict the prognosis and clinicopathological characteristics of tumor patients.Results: According to the morphological characteristics of AML, the samples were divided into 8 categories (M0 Undifferentiated, M1, M2, M3, M4, M5, M6, M7), and among them, the expression profile and expression heat map of 13 m6A RNA methylation regulators were constructed. Using m6A RNA methylation regulator as a feature vector, consistent clustering of 151 samples yielded two subgroups RM1 and RM2. Among them, the expression of the regulator in RM1 was higher than RM2,and RM2 patients had a longer survival time than RM1. Gene set enrichment analysis found that functional processes such as endothelial-hematopoietic transformation through the Notch pathway were significantly enriched in RM1. The analysis of the KM curve indicates that when the expression of FTO or ALKBH5 or of ZC3H13 was low, the survival time of patients was significantly higher than that with high expression.Conclusion: m6A RNA methylase regulator is not only an independent prognostic marker of AML, but also can predict its clinicopathological characteristics, which has potential value for stratifying prognosis and improving treatment strategies.
The distribution and abundance of multiple chalazia in pediatrics receiving surgery in Zhejiang province, China: a hospital-based cross-sectional study.
Authors: Zheng Xiaoyu , Dorazio Robert M. , Wang Tingyan , Zhao Sujuan , Zhao Zhengyan
Published: July 2020

Background: To investigate the distribution and abundance of multiple chalazia in pediatric patients that need surgical treatments.Methods: Patients with multiple chalazia treated in a tertiary Children's Hospital were reviewed between June and December 2016. Demographic data, locations and numbers of chalazia were recorded. Data were analyzed using generalized linear models of the counts and the occurrences of chalazia. Hypotheses were tested using likelihood ratio tests appropriate for each type of data.Results: The study included 128 subjects, most of which were 1-3 years of age. The majority of patients had bilateral chalazia (95.3%), and the proportions of patients with internal, external and marginal chalazion differed significantly (99.2%, 61.7% and 2.3% respectively). The total number of internal chalazia had a negative binomial distribution, whereas the total number of external chalazia followed a Poisson distribution. The number of internal and external chalazia did not differ significantly with gender, age, or residence of patients. Internal chalazia were located more frequently on the upper lids (P < 0.01). External chalazia showed no preference of localization. The average abundance of internal chalazia on each eyelid did not relate to the presence of external chalazia.Conclusions: In Zhejiang province, China, multiple chalazia that require surgery are more common among younger children (aged 1-3 years) than older ones. The anatomical distribution varies depending on the chalazion’s types. Multiple chalazia often occur bilaterally and internally. Doctors should pay more attention to the anatomical distribution to achieve a higher success rate in chalazion surgery.
Calcination Temperatures, Compositions and Antimicrobial Properties of Heterostructural ZnO–CuO Nanocomposites from Calotropis Gigantea Targeted for Skin Ulcer Pathogens
Authors: Govindasamy G Ambarasan , Mydin Rabiatul Basria S.M.N. , Harun Nor Hazliana , Sreekan Srimala
Published: July 2020

An eco-friendly green route is employed for the successful synthesis of heterostructured ZnO-CuO nanocomposites using Calotropis gigantea plant and the investigation of their antimicrobial properties against skin ulcer pathogens. Binary ZnO-CuO nanocomposites prepared at calcination temperature of 300 °C exhibited superior antimicrobial effect on S. aureus, whereas the negative control sample did not show any antibacterial activities. High ZnO nanoparticles of 75 wt.% ZnO-CuO nanocomposites showed zero count of Staphylococcus aureus at a minimum inhibitory concentration of 0.625 mg/mL and minimum bactericidal concentration (MBC) of 2.5 mg/mL. Interestingly, the 75 wt.% ZnO-CuO nanocomposites exhibited strong antimicrobial activity against multi-drug resistant pathogens, with MBC ranging from 0.3125 mg/mL to 1.25 mg/mL. A time-kill assay captured a reduction in viable count from 4.3 log 10 to 1.3 log 10 after 12 h of incubation for S. aureus . Elucidating the antimicrobial activities could be useful for identifying novel ways to incorporate ZnO-CuO nanocomposites in polymers for applications in biocide materials, such as for wound dressing. Further, molecular studies are needed to explain the underlying biocidal mechanism of ZnO-CuO nanocomposites especially in the presence of Cu 2+ and Zn 2+.
Dependence of heme biosynthesis on iron-sulfur cluster biogenesis: human ALAD is an unrecognized iron-sulfur protein
Authors: Liu Gang , Sil Debangsu , Tong Wing-Hang , Maio Nunziata , Bollinger J. Martin , Krebs Carsten , Rouault Tracey Ann
Published: July 2020

Heme biosynthesis and iron-sulfur cluster (ISC) biogenesis are two major mammalian metabolic pathways that require iron. It has long been known that these two pathways interconnect, but the previously described interactions do not fully explain why heme biosynthesis depends on intact ISC biogenesis. Herein we have identified a previously unrecognized connection between these two pathways through our discovery that human aminolevulinic acid dehydratase (ALAD), which catalyzes the second step of heme biosynthesis, is an Fe-S protein. We found that several highly conserved cysteines and an Ala306-Phe307-Arg308 motif of human ALAD are important for [Fe4S4] cluster acquisition and coordination. The enzymatic activity of human ALAD was greatly reduced upon loss of its Fe-S cluster, which resulted in reduced heme biosynthesis in human cells. Our findings explain why heme biosynthesis depends on intact ISC biogenesis, as ALAD provides an early Fe-S-dependent checkpoint in the heme biosynthetic pathway.
The proportion and effect of corticosteroid therapy for patients with COVID-19 infection: a systematic review and meta-analysis
Authors: Wang Jun-ning , Yang Wei-xia , Chen Pu-wen , Guo Jian-bin , Liu Rui , Wen Peng-fei , Li Kun , Lu Yao , Ma Tao , Song Wei , Qin Si-qing , Zhang Yu-Min , Wang Yakang
Published: July 2020

Objectives: COVID-19 remains a global challenge. Corticosteroids are a group of anti-inflammatory and suppressive immune response drugs that are widely used in the treatment of COVID-19, especially when it presents with viral pneumonia. Comprehensive reviews investigating the comparative proportion and efficacy of corticosteroid use are scarce. Therefore, we conducted a systematic review and meta-analysis of clinical trials to evaluate the proportion and efficacy of corticosteroid use for the treatment of COVID-19.Methods: We conducted a comprehensive literature review of PubMed, EMBASE, the Cochrane Controlled Trials Registry, and the China Academic Journal Network Publishing Database for relevant trials on glucocorticoid therapy in COVID-19 patients. Outcome measures were the proportion of patients administered corticosteroids, viral clearance and mortality. Effect size was reported as weighted mean differences (WMDs) for continuous outcomes and odds ratios (ORs) for dichotomous outcomes with associated 95% confidence intervals (CIs).Results: Forty-three trials involving 6603 patients were included. The meta-analysis demonstrated that the proportion of COVID-19 patients who received corticosteroids was significantly lower than that of patients who did not receive corticosteroids. In addition, our meta-analysis demonstrated no significant difference in the proportions of severe and nonsevere patients who were administered corticosteroids. We also performed subgroup analyses stratified by severity, indicating that the proportion of patients administered corticosteroids was significantly higher among intensive care unit (ICU) patients than among non-ICU patients. The results of our meta-analysis indicated that corticosteroid treatment significantly delayed the viral clearance time. Finally, our meta-analysis demonstrated no significant difference between the use of corticosteroids for COVID-19 patients who died and those who survived. This result indicated that mortality was not correlated with corticosteroid therapy.Conclusion: The proportion of COVID-19 patients who received corticosteroids was significantly lower than that of patients who did not receive corticosteroids. Corticosteroid use in subjects with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections delayed virus clearance and did not convincingly improve survival;therefore, corticosteroids should be used with caution in the treatment of COVID-19.
Macromolecule Biomaterials Promote the Osteogenic Differentiation Capacity of Equine Adipose -Derived Mesenchymal Stem Cells
Authors: Elashry Mohamed I. , Baulig Nadine , Wagner Alena-Svenja , Klymiuk Michele C. , Kruppke Benjamin , Hanke Thomas , Wenisch Sabine , Arnhold Stefan
Published: July 2020

Background: Combination of mesenchymal stem cells (MSCs) and biomaterials is a rapidly growing approach in regenerative medicine particularly for chronic degenerative disorders including osteoarthritis and osteoporosis. In the present study, the effect of biomaterial bone substitutes on equine adipose derived MSCs morphology, viability, adherence, migration and osteogenic differentiation were investigated. Methods: MSCs were cultivated in conjunction with collagen CultiSpher-S Microcarrier (MC), nanocomposite xerogels B30 and B30Str biomaterials in osteogenic differentiate medium either under static or mechanical fluid shear stress (FSS) culture conditions. The data were generated by histological means, life cell imaging, cell viability, adherence and migration assays. Osteogenic differentiation was detected by semi-quantification of alkaline phosphatase (ALP) activity, matrix mineralization using Alizarin Red S (ARS) staining and quantification of the osteogenic markers; runt related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) expression using RT-qPCR. All data were statistically analyzed using ANOVA. Results: The data revealed that combined mechanical stress with MC but not B30 enhanced MSCs viability and promoted their migration. Combined osteogenic medium with MC, B30 and B30Str increased ALP activity compared to cultivation in basal medium. Osteogenic induction with MC, B30 and B30Str resulted in diffused matrix mineralization by means of ARS. FSS increased the viability in the presence of the osteogenic medium with MC but not B30 or B30Str. FSS enhanced osteogenic differentiation in the presence of B30Str. Upregulation of Runx2 and ALP expression was detected with osteogenic differentiation together with B30 and B30Str regardless of static or FSS culture. Conclusions: Taken together, the data revealed that FSS in conjunction with biomaterials promoted osteogenic differentiation of MSCs. This combination may be considered as a marked improvement for clinical applications to cure bone defects.
TRIM16 Positively Regulates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating the Ubiquitination and Degradation of RUNX2
Authors: Zhao Yi , Zhai Qiaoli , Liu Hong , Xi Xun , Chen Shuai , Liu Dongxu
Published: July 2020

BackgroundPeriodontal disease is a common disease that compromises the integrity of tooth-supporting tissues. Bone regeneration is the ultimate goal of periodontal therapies, in which osteogenic differentiation of human periodontal ligament stem cells plays a critical role. The tripartite motif (TRIM)16 is downregulated in periodontal tissues of patients with periodontitis and involved in osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs).However, the role of TRIM16 in the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) is largely unknown.MethodshPDLSCs were isolated and identified by immunophenotype assays using flow cytometry. Overexpression plasmids and specific short-hairpin RNAs (shRNAs) were constructed to manipulate the expression of target molecules. Alkaline phosphatase (ALP) staining, alizarin red staining (ARS) and enzyme‐linked immunosorbent assays (ELISA) were used to evaluate osteogenic potential capacity. Reverse transcription quantitative PCR (RT-qPCR) and Western blot analysis were performed to determine the expression of osteogenic-related markers and activation of relevant signaling pathways. Co-immunoprecipitation assays were performed to confirm the interactions between proteins and the ubiquitination of RUNX2. A LC-MS/MS analysis was performed to explore the different expression proteins in present of TRIM16.ResultsTRIM16 significantly promoted alkaline phosphatase activity and mineralized nodule formation, and positively regulated the osteogenic differentiation of hPDLSCs by enhancing protein expression of RUNX2, COL1A1 and OCN. Mechanistically, TRIM16 serves as a pivotal factor that stabilizes RUNX2 protein levels by decreasing CHIP-mediated K48-linked ubiquitination degradation of the RUNX2 protein. Besides, TRIM16 significantly increased expression of COL1A1 via activation of p38MAPK/RUNX2.ConclusionThis study identified a novel mechanism of TRIM16 in regulating stability of the RUNX2 protein, which may promote the osteogenic differentiation of hPDLSCs. TRIM16 may be a potential target of stem cell based-bone regeneration for periodontal therapies.
Differentially expressed genes in cotyledon of ewes fed mycotoxins
Authors: Britt Jessica L , Noorai Rooksana , Duckett Susan
Published: July 2020

Background
Ergot alkaloids (E+) are mycotoxins produced by the endophytic fungus, Epichloë coenophiala, in tall fescue that are associated with ergotism in animals. Exposure to ergot alkaloids during gestation reduces fetal weight and placental mass in sheep. These reductions are related to vasoconstrictive effects of ergot alkaloids and potential alterations in nutrient transport to the fetus. Cotyledon samples were obtained from eight ewes that were fed E+ (n = 4; E+/E+) or E- (endophyte-free without ergot alkaloids; n = 4; E-/E-) seed during both mid (d 35 to 85) and late (d 85–133) gestation to assess differentially expressed genes associated with ergot alkaloid induced reductions in placental mass and fetal weight, and discover potential adaptive mechanisms to alter nutrient supply to fetus.
Results
Ewes fed E+/E + fescue seed during both mid and late gestation had 20% reduction in fetal body weight and 33% reduction in cotyledon mass compared to controls (E-/E-). Over 13,000 genes were identified with 110 upregulated and 33 downregulated. Four genes had a |log2FC|>5 for ewes consuming E+/E + treatment compared to controls: LECT2, SLC22A9, APOC3, and MBL2. REViGO revealed clusters of upregulated genes associated glucose, carbohydrates, lipid, protein, macromolecular and cellular metabolism, regulation of wound healing and response to starvation. For downregulated genes, no clusters were present, but all enriched GO terms were associated with anion and monocarboxylic acid transport. The complement and coagulation cascade and the peroxisome proliferator-activated receptor signaling pathway were found to be enriched for ewes consuming E+/E + treatment.
Conclusions
Consumption of ergot alkaloids during gestation altered the cotyledonary transcriptome specifically related to macronutrient metabolism, wound healing and starvation. These results show that ergot alkaloid exposure upregulates genes involved in nutrient metabolism to supply the fetus with additional substrates in attempts to rescue fetal growth.
A genome-wide screening for RNAi pathway genes in Acari
Authors: Soroker Victoria , Nganso Beatrice T , Sela Noa
Published: July 2020

BackgroundRNA interference (RNAi) is a highly conserved, sequence-specific gene silencing mechanism present in Eukaryotes. Three RNAi pathways critical for organismal development and survival are known, namely micro-RNA (miRNA), Piwi-interacting RNA (piRNA) and short interfering RNA (siRNA) pathways. Little knowledge exist about the genes involved in these pathways in Acari. Moreover, variable successes has been obtained in gene knockdown via siRNA pathway in functional genomics and management of Acari species. We hypothesized that the clue may be in the variability in the composition and the efficacy of siRNAi machinery among Acari. ResultsBoth comparative genomic analyses and domain annotation suggest that all the analyzed species have orthologs of genes that mediate gene silencing via the three RNAi pathways though gene duplication and/or loss have occurred in the different species. We also identified orthologs of Caenorhabditis elegans RNA-dependent RNA polymerase (RdRP) gene in all Acari species though no secondary Argonaute homologs that operate with this gene in siRNA amplification mechanism were found. This finding suggests that the siRNA amplification mechanism present in Acari may be distinct from that described in C. elegans. Moreover, the genomes of these Acari species encode no ortholog of C. elegans systemic RNAi defective 1 (Sid-1) that mediate systemic RNAi, suggesting that the phenomena of systemic and parental RNAi that has been reported in some Acari species probably occur through a different mechanism. Orthologs of RNAi spreading defective-3 (Rsd-3) gene and scavenger receptors namely Eater and SR-CI that mediate endocytosis cellular update of dsRNA in C. elegans and Drosophila melanogaster were found in Acari genomes. This result suggests that cellular dsRNA uptake in Acari is endocytosis-dependent. Detailed phylogenetic analyses of core RNAi pathway genes in the studied Acari species revealed that their evolution is compatible with the proposed monophyletic evolution of this group.ConclusionsTaken together, our in silico comparative analyses have revealed the potential activity of all three pathways in Acari. However, much experimental work remains to be done in elucidating the operating mechanisms behind these pathways in particular those that govern systemic/parental RNAi and siRNA amplification in Acari.
The Role of Age Distribution, Time Lag Between Reporting and Death and Healthcare System Capacity in Case Fatality Estimates of COVID-19
Authors: Vanella Patrizio , Wiessner Christian , Holz Anja , Krause Gérard , Möhl Annika , Wiegel Sarah , Lange Berit , Becher Heiko
Published: July 2020

Background: European countries report large differences in coronavirus disease (COVID-19) case fatality risk (CFR). CFR estimates depend on demographic characteristics of the cases, time lags between reporting of infections and deaths and infrastructural characteristics, such as healthcare and surveillance capacities. Methods: We used publicly available data from official reports of the national health authorities of Germany, Italy, France, and Spain on COVID-19. These include age-specific numbers of cases and deaths for different dates, which we used to compute age-standardized CFR ratios using a standard European population for standardization. Moreover, we investigated the impact of different potential time lags on the estimation of the CFR using data published by the European Centre for Disease Prevention and Control (ECDC). Finally, we described the association between case fatality and the intensive care bed capacity.Results: We found that age-standardized CFR estimates increased from the beginning of March to mid-May 2020 in all included European countries. In Germany, CFRs are lower than in other countries. However, the differences are much larger when comparing the crude risks rather than the age-adjusted risks. Thus, the different age distribution of the cases account for a major proportion of the reported differences. Case fatality estimates using time lags of 1-10 days converged in all countries over time, however, there is no optimal time lag to assess the CFR during the pandemic. Time lags that provided the most constant estimates and approach best the observed CFR after the pandemic ranged from 5-10 days in different countries and at different time points during the pandemic. For the association between intensive care bed capacity and fatality we found that days with a high need for intensive care beds were positively correlated with daily hospitalization fatality in France, Italy, and Spain, but not in Germany. Conclusions: Our results highlight that cross-country comparisons of crude CFR estimates can be misleading and should be avoided. However, to adjust for potential sources of bias more disaggregated data and information on surveillance and health care capacities are needed. Filling these gaps and harmonizing data across European countries will facilitate further analysis.
Serum miR-218 is a Potential Biomarker in the Diagnosis of Colorectal Cancer
Authors: Zhang Keqian , Mao Tianqi , He Zhicheng , Wu Xiaojiao , Peng Yu , Chen Yanrong , Dong Yan , Ruan Zhihua , Wang Zhe
Published: July 2020

Background: Our study was designed to explore the diagnostic role of serum microRNA-218 (miR-218) in colorectal cancer (CRC).Methods: Serum level of miR-218 was measured in 117 CRC samples and 88 normal controls using quantitative reverse transcription (qRT-PCR). Chi-square test was performed to assess the relationships between miR-218 expression and clinical characteristics of CRC patients. The receiver operating characteristic (ROC) analysis was established to investigate the diagnostic significance of miR-218 in CRC with the area under ROC curve (AUC).Results: MiR-218 was found to be weekly expressed in CRC serum samples compared with healthy controls (P < 0.001). And the down-regulation of miR-218 shared close relationships with lymph node metastasis (P = 0.013), vascular invasion (P = 0.020) and TNM stage (P = 0.031). However, age, tumor size and gender had no significant influence on miR-218 expression (P > 0.05). According to the ROC curve, miR-218 yielded an AUC value of 0.897 and an optimal cutoff point value of 0.021, providing a 83.8% sensitivity and a 78.4% specificity. Conclusion: In summary, decreased expression of serum miR-218 was detected in CRC patients and the expression of miR-218 was a diagnostic marker in CRC.
Carbon Stock Variation along altitudinal gradient of Wacho Forest in Hawa Galan District, Kellem Wollega Zone, Oromia Region, Ethiopia
Authors: Gashu Gezahegn , Gidey Gebre , Deressa Sime , Fekansa Zame , Awoke Halid
Published: July 2020

Background

Forest ecosystem plays a crucial role in the global carbon cycle; as such, mitigating high atmospheric concentrations of carbon dioxide and other greenhouse gases by naturally taking carbon from the atmosphere through photosynthesis. Verification and accounting of carbon stock in forest ecosystem have been renowned as a potential strategy to reduce and stabilize atmospheric concentrations of greenhouse gas. Forest sequesters and store more carbon than any other terrestrial ecosystem and it is an important natural break on climate change. It acts as a carbon reservoir by storing large amount of carbon in trees, undergrowth vegetation, forest floor and soil.
Result

The mean carbon stock of each carbon pool was changed along altitudinal class of the study area. The largest mean above and below ground carbon stock was found in the second altitudinal class(1560.01-1643m) followed by first altitudinal class(1435-1560m) and the third altitudinal class (1643.01-1704m) of the study area. The largest mean dead tree and dead wood carbon stock was also stored in the first altitudinal class followed by the third and the second altitudinal class of the study area. The largest mean litter carbon stock was found in the first altitudinal class followed by the second and the third altitudinal class of the study area. The largest mean soil organic carbon was found in the third altitudinal class followed by the second and the first altitudinal class of the study area.
Conclusions

The carbon stock variation along altitudinal gradients indicated that, altitude had no a statistically significant effect on any of the carbon pools except litter carbon of the study area at 95% of confidence interval.
P2X7 receptor/NLRP3 inflammasome complex and a-synuclein/parkin balance in neo-diagnosed, treatment-naïve Parkinson disease: a prospective study
Authors: Solini Anna , Rossi Chiara , Santini Eleonora , Giuntini Martina , Raggi Francesco , Parolini Federico , Biancalana Edoardo , Prete Eleonora Del , Bonuccelli Ubaldo , Ceravolo Roberto
Published: July 2020

Background

Neuro, and likely systemic inflammation, with abnormal α-synuclein deposition, participate in the development of Parkinson’s disease (PD). The P2 × 7 receptor/NLRP3 inflammasome complex is upregulated in the brain of PD patients. Aim of this study was to explore whether the systemic activation of such complex might participate in the pathogenesis of PD.
Methods

Systemic expression and functional activity of the inflammasome were measured in 25 newly diagnosed PD patients and 25 controls at baseline and after twelve months of pharmacologic treatment, exploring the involved intracellular signalling and its epigenetic regulation. A putative mechanistic explanation of the results was validated in a murine model of neuroinflammation.
Results

De-novo PD patients were characterized by a systemic hyper-expression of the P2 × 7R/NLRP3 inflammasome platform, likely modulating circulating and lymphomonocyte α-synuclein, whose deposits represent the main pathogenetic factor of PD. A reduced JNK phosphorylation might be the involved intracellular signalling. miR-7 and miR-30, implied in the pathogenesis of PD and in the post-transcriptional control of α-synuclein and NLRP3 expression, were also increased in PD. After one year of usual anti-Parkinson treatments, such inflammatory platform was significantly reduced. In the substantia nigra of P2 × 7R KO mice, a neuroinflammatory stimulus induced a strong expression of parkin, a protective protein, suggesting that P2 × 7R activation might participate in the inflammatory damage occurring in specific brain areas also by inhibiting parkin expression.
Conclusion

Newly-diagnosed PD subjects display a systemic hyper-expression of the P2 × 7R/NLRP3 inflammasome platform that seems to modulate circulating and lymphomonocyte α-synuclein; a reduced JNK phosphorylation might be the intracellular signalling mediating this effect, undergoing an epigenetic regulation by miR-7 and miR-30.
Trial registration
ClinicalTrials.gov (NCT03918616).