bluuebunny/crossref_metadata_2025 huggingface dataset:
Kemijska analiza hlapljivih spojeva tradicionalne rakije Anižete s otoka Korčule – Republika Hrvatska
Authors: Šarolić Mladenka , Marijanović Zvonimir , Dorbić Boris , Svalina Tomislav , Šuste Marko , Delić Žana , Friganović Emilija , Bosnić Nikolina
Published: June 2020

Anižeta je prirodno jako alkoholno piće koje se proizvodi aromatiziranjem rakije lozovače prirodnim ekstraktima zvjezdastog anisa (llicium verum Hook.), anisa (Pimpinella anisum L.), koromača (Foeniculum vulgare Mill.) ili drugog bilja koje sadržava jednake glavne
aromatične sastojke. U brojnim zemljama mediteranskog područja rakije aromatizirane anisom imaju dugu tradiciju proizvodnje. U našoj zemlji Anižeta je tradicionalno jako alkoholno piće koje se najvećim dijelom proizvodi na otoku Korčuli. Tijekom proizvodnje rakije nastaju brojne hlapljive tvari čiji udjel i sastav određuje kvalitetu gotovog proizvoda. Cilj ovog rada je utvrditi profil hlapljivih sastojaka rakije
Anižete. Za izolaciju hlapljivih spojeva korištena je tehnika mikroekstrakcije vršnih para na krutoj fazi (HS-SPME). Analiza izoliranih hlapljivih spojeva provedena je primjenom plinske kromatografije udružene s spektrometrijom masa (GC-MS). Identificirano je ukupno 26 hlapljivih spojeva koji pripadaju sljedećim kemijskim skupinama: esteri, viši alkoholi, terpeni, fenilpropani i norizoprenoidni derivati. Brojčano su najzastupljeniji esteri, a fenilpropani cis-anetol (17,88 %) i trans-anetol (31,62 %) su prisutni u
najvišem relativnom udjelu te doprinose slatkastoj, specifičnoj aromi Anižete. Dobiveni rezultati su uspoređeni sa literaturnim podacima sličnih alkoholnih pića u drugim zemljama.
- Musik Mozart Memiliki Ekspresi Brain Deriverd Neurotrophic Factor Tertinggi Pada Serebrum dan Serebellum Rattus norvegicus Dibandingkan Dengan Gamelan Jawa, Sunda Dan Bali Selama Kehamilan
Authors: Widoyanti Virki , J Hermanto Tri , Widjiati Widjiati
Published: June 2020

Pendahulan: Musik dapat memengaruhi sistem saraf parasimpatis atau sistem saraf otomatis, baik secara langsung maupun tidak langsung. Paparan musik Mozarts, gamelan Jawa, Sunda, dan Bali memengaruhi faktor neurotropik yang ada di otak. Metode: Penelitian ini adalah eksperimental laboratorium dengan desain random post-test onlydalam desain kelompok kontrol yang digunakan oleh hewan percobaan Rattus norvegicus sebagai model. Hasil: Ada perbedaan ekspresi brain deriverd neurotrophic factor yang signifikan pada  anak Rattus norvegicus antara kelompok dengan p = 0,018 (rata-rata 1,60 ± 0,42 untuk kelompok kontrol, 4,00 ± 1,85 untuk kelompok musik mozart, 3,00 ± 0,96 untuk orang Jawa Kelompok gamelan, 2,48 ± 0,68 kelompok Gamelan Sunda, dan 2,16 ± 1,27 pada kelompok Gamelan Bali) juga di otak kecil dengan p = 0,022 (rata-rata 1, 44 ± 0,29 untuk kelompok kontrol, 3,92 ± 1,81 untuk acara Mozart, 3.04 ± 0.94 grup gamelan Jawa, 2.92 ± 0, 41 grup gamelan Sunda, dan 2.57 ± 1.053 grup gamelan Bali). Kesimpulan: Ada perbedaan ekspresi brain deriverd neurotrophic factor pada serebrum dan serebellum Rattus norvegicus antara yang terpapar musik Mozart, gamelan Jawa, Sunda, Bali dan kontrol.

Key words :  Mozart, Javanese Gamelan, Sundanese Gamelan, Balinese Gamelan, Brain Deriverd Neurotrophic Factor
Solubilisasi Fosfat Anorganik oleh Burkholderia spp. pada Rizosfer Kelapa Sawit (Elaeis guineensis Jacq.) di Tanah Mineral Masam
Authors: Nugraha Gregorius Baskara Aji , Wandri Ruli , Asmono Dwi
Published: June 2020

Nugraha et al, 2019. Solubilization of Inorganic Phospate by Burkholderia spp. Associated with Oil Palm Rhizosphere in Mineral Acid Soil. JSLO 8(1):86-93.Phosphate Solubilizing Bacteria (PSB) play important role by enhancing phosphate availability bounded with Al3+ or  Fe3+ in acidic soils to oil palm plants through release the inorganic phosphate by enzyme or organic acids solubilization. The aims of this study were to isolate of PSB from oil palm rhizosphere and to conduct a comparative analysis of the solubility inorganic phosphates source by selected PSB. The ability of 15 selected PSB to grow and solubilize aluminum phosphate (AlPO4) and iron phosphate (FePO4) was examined and identified. The highest phospate solubilising efficiency showed K3.1 isolate with phosphate solubilization index 3.2 on NBRIP media. Quantitative analysis revealed that isolate K3.1 solubilized 53.52 mg/mL phosphate in 5 days after being inoculated in AlPO4 containing liquid medium, isolate A4 solubilized 63.45 mg/mL phosphate in 5 days after being inoculated in FePO4 containing liquid medium accompanied by a decrease in pH of the growth medium. Based on the 16s rRNA gene sequence analysis, isolate K3.1 and A.4 were closely related to Burkholderia arboris and Burkholderia gladioli. This potential isolates can be used in order to make oil palm crops more sustainable especially on marginal soil with low pH and less dependent on inorganic P fertilizers.
Akumulasi Logam Berat Zn dan Pb pada Sedimen, Akar dan Daun Mangrove Avicennia alba di Pulau Payung, Sumatera Selatan
Authors: Riyanti Ida , Putri Wike Ayu Eka , Ulqodry Tengku Zia , Santeri Tiara
Published: June 2020

Riyanti I, Putri WAE, Ulqodry TZ, Santeri T. 2019. Accumulation of heavy metal Zn and Pb in sediment, root and leaves of mangrove Avicennia alba in Payung Island, South Sumatra. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands. 8(2):141-147. Various industrial activities or natural activity in the Musi River, might contribute to the heavy metals input in mangrove area of Payung Island. The purpose of this study was to analyze the concentration of  heavy metals zinc (Zn) and lead (Pb) in sediment, root and leaves of mangrove A. alba. This research was conducted in January 2018. Analysis of heavy metal used Atomic Absorption Spectrophotometry (AAS). The results indicated that Zn concentration of sediment (13.35-22.61 mg/kg) and Pb (0.14-3.17 mg/kg), was still in quality standard level. Zn concentration in roots was 0.45-4.95 mg/kg and in leaf was Zn 2.46-12.76 mg/kg, while Pb on root and leaves was not detected.  The value of BCF roots, leaf BCF and TF values were 0.15-0.24 ; 0.30-0.59 ; and 1.69-2.47, respectively. The results of this study indicated that mangrove A. alba in Payung Island as moderate accumulator plants category, and plants could transmit heavy metals effectively.
Respon Penambahan Pupuk Hayati terhadap Pertumbuhan dan Hasil Padi Sawah
Authors: Jumakir Jumakir , Endrizal Endrizal
Published: June 2020

Jumakir J, Endrizal E. 2019. Response of biological fertilizer addition to growth and results of rice field. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands. 8(2):173-180. The research objectives were to determine the effect or response of the addition of biological fertilizers to the growth and yield of rice, to determine the most effective type of biological fertilizer. This study was conducted in irrigated rice field of Sri Agung Village, Batang Asam sub District, West Tanjung Jabung District, Jambi Province in dry season 2016. The design was Randomized Block Design (RBD) with six treatments and three replications with 5 mx 10 m plot area, implemented in a farmer area of 1200 m2. The results of the analysis based on the analysis of variance showed significant and unreal differences in growth and yield of rice. The addition of biological fertilizer provided higher growth and yields than without biological fertilizer. Provibio biofertilizer is the most effective biofertilizer that can provide the highest rice yield and the use of biofertilizer can save 50% recommended NPK fertilizer.
Effectiveness of the Ageratum (Ageratum conyzoides) Leaf Extract as Botanical Fungicide Against Twisted Disease of Shallot
Authors: Rianosa Rianosa , Hartal Hartal , Setyowati Setyowati
Published: June 2020

Rianosa R, Hartal H, Setyowati N. 2020. Effectiveness of the Ageratum (Ageratum conyzoides) leaf extract as botanical fungicide against moler diseases of shallot. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands 9(1): 1-10.  Moler is a major disease of onion caused by Fusarium oxysporum f. sp. cepae. Application of synthetic fungicides in the long-term has a negative effect to the environment. Weed leaves of Ageratum (Ageratum conyzoides) have potential to be an anti-fungal, anti-bacterial as well as anti-cancer. This study aimed to determine the best concentration of Ageratum leaf extract to control twisted disease and to compare the effectiveness of botanical fungicide and synthetic fungicide. The research was conducted in June - September 2018 at the Plant Protection Laboratory and greenhouse, University of Bengkulu. The experiment was consists of five Ageratum leaf extract concentrations as a botanical fungicide (i.e. 1%, 2%, 3%, 4%, and 5%), and synthetic fungicide namely mancozeb and metil tiofanat and control treatment (in sterile soil with no fungicide treatment). The experiment used Completely Randomized Design (CRD) with a single factor. Ageratum leaf extract was obtained through the maceration method using ethanol 96%. The application of fungicide was carried out on shallot bulbs before planting. The results showed botanical fungicides from Ageratum leaf extract were effective against twisted disease of shallots, concentration 4% of Ageratum leaf extract resulted in a longer incubation period and lower disease intensity compared to other concentrations, and the effectiveness of Ageratum leaf extract at a concentration of 4% was comparable to that of mancozeb to control twisted disease. Application of both synthetic and botanical fungicide resulted in a higher yield of shallot. Therefore, Ageratum leaf extract has potential as a substitution of synthetic fungicides to control twisted disease of shallots.
Ajattelun ajattelua
Authors: Koikkalainen Riitta
Published: June 2020

Kirja, joka opettaa paljon mutta ei mestaroi. Työkseen kirjoittavan self-help, kiinnostava ja oivalluksiin herättävä teos kaikille kielen käyttäjille. Sellainen on Kersti Juvan kirja Löytöretki suomeen.
Analisis Deskriptif ISPA pada Anak dan Balita di Pulau Moti
Authors: Hasan Marhaeni , The Fera
Published: June 2020

Acute Respiratory Infection (ARI) continues to be the leading cause of death in infants and children under the age of five throughout the world. ARI is responsible for the deaths of around 3.9 million children worldwide each year. It was reported that around 40% of total deaths from ARI around the world occurred in Bangladesh, India, Indonesia, and Nepal. The results of the 2013 Basic Health Research (Riskesdas) report showed that the prevalence of ARI in Indonesia is still quite high at 25%. The highest prevalence occurred in infants (25.8%) and infants (22%). This study aims to determine the factors that influence the incidence of acute respiratory infections (ARI) in children 0-5 years on Moti Island. This study uses an observational study using a cross-sectional approach. The samples used were 100 people with an age range of 0-5 years. The data used in the form of a questionnaire. The results showed that education occupying the highest percentage was elementary school (SD) graduates, 38%, while the percentage according to employment was as follows, the highest percentage was housewives (83%), the majority of toddlers born weighing over 2500 grams (89%), complete immunization 81%, exclusive breastfeeding has also reached 76%, 81% of these toddlers live in families who have smoking habits, 79% of respondents still use fuel, 80% of parents whose environment around the house is dusty. The conclusion that can be drawn is the risk factors that most influence the incidence of ARI in infants on Moti Island are environmental factors, namely the level of occupancy density, dusty house conditions, the use of firewood as fuel for cooking, and smoking habits of family members in the house.
Separation of Mycobacterium smegmatis From a Mixed Culture Using the Cell Wall Binding Domain of D29 Mycobacteriophage Endolysin
Authors: Nair Gokul , Jain Vikas
Published: June 2020

Pathological infection caused by Mycobacterium tuberculosis is still a major global health concern. Traditional diagnostic methods are time-consuming, less sensitive, and lack high specificity. Due to an increase in the pathogenic graph of mycobacterial infections especially in developing countries, there is an urgent requirement for a rapid, low cost, and highly sensitive diagnostic method. D29 mycobacteriophage, which is capable of infecting and killing M. tuberculosis, projects itself as a potential candidate for the development of novel diagnostic methods and phage therapy of mycobacterial infections. In our previous study, we showed that the cell wall binding domain [C-terminal domain (CTD)] located at the C-terminal end of the D29 mycobacteriophage LysA endolysin very selectively binds to the peptidoglycan (PG) of Mycobacterium smegmatis and M. tuberculosis. Here, by using M. smegmatis as model organism and by exploiting the PG binding ability of CTD, we have developed a method to isolate M. smegmatis cells from a mixed culture via magnetic separation. We show that green fluorescent protein (GFP)-tagged CTD (CTD-GFP) can bind to M. smegmatis cells in vitro after treatment with non-ionic detergent Triton X-100. Fluorescence-based assays show that CTD-GFP binding to M. smegmatis cells is highly specific and stable, and is not disrupted by an excess of either GFP or BSA. We further fused CTD with glutathione-S-transferase (GST) to generate CTD-GST protein and carried out an anti-GST antibody-mediated coating of CTD-GST on Dynabeads. This allowed us to perform successful magnetic separation of M. smegmatis from a mixed culture of bacteria having both Gram-negative and Gram-positive bacteria. Furthermore, the separated cells could be confirmed by a simple PCR. Thus our assay allows us to separate and identify M. smegmatis from a mixed culture.
Technologies for Sustainable Biomass Supply—Overview of Market Offering
Authors: Flak Jacek
Published: June 2020

This article introduces a collection of Information and Communications Technologies (ICT), Internet of Things (IoT), and Industry 4.0 technologies utilized in (or applicable to) biomass supply chains that constitute the current state-of-the-art along with their brief descriptions. The scoping of technologies has been conducted by means of direct interactions with bioeconomy stakeholders and technology providers, analysis of the reports from bioeconomy-related projects, literature surveys, and internet searches. It is to highlight that technology mapping investigated here is focused on commercially available tools and services, which usually come with support, thus removing the necessity for expert knowledge or unusual technical proficiency. The list with over 100 items represents the current best knowledge of its creators and it is currently available through the ICT-BIOCHAIN project platform, serving as a database with technology descriptions and capability for updating the information. The ultimate objective of the database and the platform is to serve as a common point facilitating the cross-sectorial connection, where biomass stakeholders looking for new ICT, IoT, and Industry 4.0 solutions to make their work more efficient and sustainable can browse and filter out the records of their interest and obtain the contact information of the providers.
The Effect of Cropping Method and Botanical Form on Seed Yielding and Chemical Composition of Chickpeas (Cicer arietinum L.) Grown under Organic System
Authors: Księżak Jerzy , Bojarszczuk Jolanta
Published: June 2020

A field study was conducted at the Agricultural Experimental Station in Grabów in Poland between 2017–2018. This study evaluated seed yield and chemical composition of chickpeas (Cicer arietinum L.) under organic conditions, either growing as a sole crop, or with barley (Hordeum vulgare) or oats (Avena sativa L.) as supporting plants. Two chickpea types were included in experiment scheme: kabuli and desi. The experiment was established as a split-plot design with four replicates. The study showed that a higher total seed yields of both forms of chickpeas grown in both pure sowing and with spring cereals was obtained in 2018 than 2017. The higher yield in this study period was the result of a greater number of pods, seeds, and higher weight of the chickpea seed and cereal grains on a plant. Higher yields were noted in chickpeas grown with supporting crops than in sole cropping. Significantly better thousand seed weight of both botanical forms of chickpeas was observed in chickpeas grown in sole cropping than with supporting plants. Regardless of cropping method, the desi form was characterized by higher yields than the kabuli type, and its percentage in seed yields of chickpeas grown with cereals was higher than the kabuli type. The highest seed yields were obtained in chickpeas grown with oats. Neither chickpea type had a significant effect on the height of cereal plant, the number of grains on each plant, the number of producing shoots or thousandgrainweight of the two cereal species. Regardless of cropping method, the highest content of fiber and fat was determined in desi-type seeds, while the highest protein and phosphorus content was characterized kabuli-type seeds.
Volatile Profile of Wall Rocket Baby-Leaves (Diplotaxis erucoides) Grown under Greenhouse: Main Compounds and Genotype Diversity
Authors: Guijarro-Real Carla , Rodríguez-Burruezo Adrián , Fita Ana
Published: June 2020

Wall rocket is a leafy vegetable with pungent flavor related to the presence of isothiocyanates (ITCs). Despite interest in it as a crop of high organoleptic quality, the variability of the volatile profile in the species remains unknown. Twenty-four populations grown under a greenhouse were evaluated. A considerable diversity for the total levels of volatiles was found, providing information of the aroma intensity among accessions. ITCs represented the main fraction. Allyl ITC was the main compound, and levels showed up to 6-fold difference among populations. The esters fraction was mainly represented by cis-3-hexenyl isovalerate and cis-3-hexenyl butyrate, with 20-fold differences among populations. Additionally, the content in sinigrin was evaluated as main GSL in wall rocket. Differences reached up to 13-fold. These results suggest that some populations can be used to develop highly pungent varieties, whereas some others can be selected for mild-pungent varieties, as it is the case of DER045 with low levels of ITCs and high in esters. The presence of several ITCs in the profile also suggested the presence of other novel GSLs. Overall, the work increases the knowledge in the variability of wall rocket for the volatile profile and sinigrin accumulation, a starting point for future breeding programs.
Performance of Slow-Growing Male Muscovy Ducks Exposed to Different Dietary Levels of Quebracho Tannin
Authors: Castillo Annelisse , Schiavone Achille , Cappai Maria Grazia , Nery Joana , Gariglio Marta , Sartore Stefano , Franzoni Alessandro , Marzoni Margherita
Published: June 2020

The study of the nutritional effects of tannins is complex due to the large chemical diversity; consequently, in poultry nutrition the biological responses may vary greatly. The aim of the present study was to evaluate the effect of different levels of dietary Quebracho tannins (QT) on growth and production performance in slow-growing type Muscovy ducks. For this purpose, a 42-d trial was carried out on 126 male ducks (42-d old at start), fed on three levels of dietary QT inclusion in the diet (0% as control diet, vs. 1.5% vs. 2.5% on an as fed basis). Birds were reared under free-range conditions. A linear increase in feed intake as a function of QT inclusion in the diet was observed (p < 0.05). No difference as to final body weight, overall average daily weight gain (ADG) and total feed conversion ratio (FCR) in relation to dietary treatments was observed. Carcass yields were positively improved in QT birds (p < 0.05). No adverse responses were recorded in total blood protein and liver weight. Dietary QT might be safely used up by to 2.5% in 42- to 84-d aged male Muscovy ducks.
Process Monitoring of Antisolvent Based Crystallization in Low Conductivity Solutions Using Electrical Impedance Spectroscopy and 2-D Electrical Resistance Tomography
Authors: Rao Guruprasad , Aghajanian Soheil , Koiranen Tuomas , Wajman Radosław , Jackowska-Strumiłło Lidia
Published: June 2020

Industrial process monitoring is an important field of research where different chemical processes are monitored and controlled. In this work, electrical impedance spectroscopy (EIS) was used to analyze antisolvent based crystallization of sucrose solutions. The impedance and phase spectra were recorded for four known sucrose concentrations in water, and for each case, four predetermined amounts of ethanol were added. As a result, sixteen different solutions involving sucrose solutions of different concentrations and ethanol to water ratios were analyzed. Significant differences were observed in the magnitude and phase spectra of the solutions in the frequency range of 50 kHz to 300 kHz. The experimentally obtained data from the EIS were converted into frequency response models. Three continuous-time transfer function models of the first-order, second-order, and a second-order with a zero were estimated and compared. In addition, a 2-D electrical resistance tomography (ERT) system with a low conductivity sensor unit was designed and tested with demineralized water, tap water and industrial food grade saturated sucrose solution. Non-conducting phantom and sugar crystals were observed within the saturated sucrose solution using the Bayesian reconstruction algorithm. These demonstrations have the potential to be developed into a multi-frequency ERT systems for monitoring the distribution of the crystals in the reactor. The EIS modality can be a complementary process analytical technology (PAT) tool indicating supersaturation status and provide quality assurance.
Lipidic Matrixes Containing Lemon Essential Oil Increases Storage Stability: Rheological, Thermal, and Microstructural Studies
Authors: Rojas John , Cabrera Sergio , Ciro Gelmy , Naranjo Ana
Published: June 2020

Lemon essential oil (LEO) is known for having excellent antioxidant properties with applications in the pharmaceutical, food and cosmetic sectors. However, its deficient stability to light and temperature compromise this activity. In this study, waxy emulsifiers, such as beewax, candelilla wax, carnauba wax, and ozokerite wax, were employed in order to create lipidic matrixes with LEO as a continuous phase by heating to 80 °C under homogenization, followed by rapid cooling to 25 °C. The thermal, microstructural, rheological, and antioxidant tests were then conducted on such matrixes. The difference in melting, crystallization, and gelling temperatures was explained by the diverse chemical composition (long-chain wax esters in carnauba wax having a high melting point, and short-chain fatty acids and hydrocarbons in beewax and ozokerite wax, respectively). Crystal habits of these matrix systems varied from grainy, oval, to fiber-like shape, whereas there was a prevalent orthorhombic allomorph. The alignment and reorganization of carnauba and candelilla wax crystals led to an increase in the matrix strength as compared to those of beeswax and ozokerite matrixes, which showed a weak texture and poor crystallization behavior. The last two matrix systems showed the best flow and extensibility. These lipidic matrixes showed potential use for topical applications having good oil retention capacity and better antioxidant properties as compared to the raw essential oil.
Influence of Enzyme Additives on the Rheological Properties of Digester Slurry and on Biomethane Yield
Authors: Müller Liane , Engler Nils , Rostalsky Kay , Müller Ulf , Krebs Christian , Hinz Sandra
Published: June 2020

The use of enzyme additives in anaerobic digestion facilities has increased in recent years. According to the manufacturers, these additives should increase or accelerate the biogas yield and reduce the viscosity of the digester slurry. Such effects were confirmed under laboratory conditions. However, it has not yet been possible to quantify these effects in practice, partly because valid measurements on large-scale plants are expensive and challenging. In this research, a new enzyme product was tested under full-scale conditions. Two digesters were operated at identic process parameters—one digester was treated with an enzyme additive and a second digester was used as reference. A pipe viscometer was designed, constructed and calibrated and the rheological properties of the digester slurry were measured. Non-Newtonian flow behavior was modelled by using the Ostwald–de Baer law. Additionally, the specific biomethane yield of the feedstock was monitored to assess the influence of the enzyme additive on the substrate degradation efficiency. The viscosity measurements revealed a clear effect of the added enzyme product. The consistency factor K was significantly reduced after the enzyme application. There was no observable effect of enzyme application on the substrate degradation efficiency or specific biomethane yield.
Climate Adaptation in Maintenance Operation and Management of Buildings
Authors: Grynning Steinar , Gradeci Klodian , Gaarder Jørn Emil , Time Berit , Lohne Jardar , Kvande Tore
Published: June 2020

The aim of this paper is to analyze the basic criteria, trends, applications, and developments related to climate adaptation in building maintenance and operation management (MOM) practices in Norway. Investigations conducted as part of the study include an analysis of current literature addressing climate adaptation in relation to MOM practices, supplemented by a review of existing research projects and initiatives in this field. Three case studies involving different Norwegian building owner organizations were examined in order to investigate the current status of the application and extent of climate adaptation practices in relation to MOM. The study has revealed a significant gap between theory and practice when it comes to integrating MOM in relation to climate adaptation. The concept of climate adaptation is only addressed as a high-level strategic issue. The case studies thus emphasize the need for a structured process that can enable the incorporation of climate adaptation in current MOM practices. This proposes a generic and structured climate-adaptive MOM framework that will enable the incorporation of climate adaptation in into corporate MOM practices at different scales and organizational levels. Implementation of this flexible and transferable framework is expected to provide a basis for accruing further knowledge on climate adaptation. Further work with the framework should include the introduction of more tangible and tailored tools and processes, including checklists or scoring systems accompanied by relevant climate adaptation factors and plans.
Redirecting T Cells against Epstein–Barr Virus Infection and Associated Oncogenesis
Authors: Münz Christian
Published: June 2020

The Epstein–Barr virus (EBV) is associated with lymphomas and carcinomas. For some of these, the adoptive transfer of EBV specific T cells has been therapeutically explored, with clinical success. In order to avoid naturally occurring EBV specific autologous T cell selection from every patient, the transgenic expression of latent and early lytic viral antigen specific T cell receptors (TCRs) to redirect T cells, to target the respective tumors, is being developed. Recent evidence suggests that not only TCRs against transforming latent EBV antigens, but also against early lytic viral gene products, might be protective for the control of EBV infection and associated oncogenesis. At the same time, these approaches might be more selective and cause less collateral damage than targeting general B cell markers with chimeric antigen receptors (CARs). Thus, EBV specific TCR transgenic T cells constitute a promising therapeutic strategy against EBV associated malignancies.
Targeting Cancer Stem Cells to Overcome Therapy Resistance in Ovarian Cancer
Authors: Muñoz-Galván Sandra , Carnero Amancio
Published: June 2020

Ovarian cancer is the most lethal gynecological malignancy due to its late detection and high recurrence rate. Resistance to conventional platinum-based therapies and metastasis are attributed to a population of cells within tumors called cancer stem cells, which possess stem-like features and are able to recapitulate new tumors. Recent studies have deepened the understanding of the biology of ovarian cancer stem cells and their special properties and have identified multiple markers and signaling pathways responsible for their self-renewal abilities. Targeting cancer stem cells represents the most promising strategy for overcoming therapy resistance and reducing mortality in ovarian cancer, but further efforts must be made to improve our understanding of the mechanisms involved in therapy resistance. In this review, we summarize our current knowledge about ovarian cancer stem cells, their involvement in metastasis and their interactions with the tumor microenvironment; we also discuss the therapeutic approaches that are being developed to target them to prevent tumor relapse.
Ion Charge Influence on the Molecular Structure of Polyethylene Terephthalate Films after Irradiation with Swift Heavy Ions
Authors: Tuleushev Adil Z. , Zdorovets Maxim V. , Kozlovskiy Artem L. , Harrison Fiona E.
Published: June 2020

We report here experimental results investigating the influence of the initial swift heavy ion charge on the structure of polyethylene terephthalate (PET) film after irradiation, using a structurally sensitive X-ray diffraction method. Kr ions with an energy of 100 MeV and charges of 13+, 14+, and 15+ were each used at irradiation fluences of 5 × 1010, 7.5 × 1010, 1 × 1011, 2.5 × 1011 and 5 × 1011 ions/cm2. At constant energy and irradiation fluence, the post-irradiation structural changes in PET film show a clear dependence on the initial ion charge. As either the fluence or ion charge increase, the latent tracks begin to overlap, leading to cross-linking of PET chain molecules to form rotational isomers (rotamers). We use the fluence corresponding to the onset of overlapping to estimate the size of latent tracks for different ion charges. At the highest fluences, the latent tracks become entirely overlapped, and the interchain cross-linking extends throughout the whole film. Since this cross-linking is due to the dipole–dipole interaction of subunits of repeat units of PET chain molecules, it is reversible, in contrast to the well-known chemical cross-linking of polymer chain molecules under irradiation.
The Cost for Biodiversity: Records of Ciliate–Nematode Epibiosis with the Description of Three New Suctorian Species
Authors: Baldrighi Elisa , Dovgal Igor , Zeppilli Daniela , Abibulaeva Alie , Michelet Claire , Michaud Emma , Franzo Annalisa , Grassi Eleonora , Cesaroni Lucia , Guidi Loretta , Balsamo Maria , Sandulli Roberto , Semprucci Federica
Published: June 2020

Epibiosis is a common phenomenon in marine systems. In marine environments, ciliates are among the most common organisms adopting an epibiotic habitus and nematodes have been frequently reported as their basibionts. In the present study, we report several new records of peritrich and suctorian ciliates-nematode association worldwide: from a deep-sea pockmark field in the NW Madagascar margin (Indian Ocean), from a shallow vent area in the Gulf of Naples (Mediterranean, Tyrrhenian Sea), in a MPA area in the Gulf of Trieste (Mediterranean, Adriatic Sea), from a mangrove system in French Guiana (South America, Atlantic Ocean), and from the Maldivian Archipelago. In addition, three new species of Suctorea from the Secca delle Fumose shallow vent area (Gulf of Naples) were described: Loricophrya susannae n. sp., Thecacineta fumosae n. sp. and Acinetopsis lynni n. sp. In the light of these new records and data from the existing literature, we discuss the suctorian–nematode epibiosis relationship as a lever to biodiversity.
Novel Protocol for Estimating Viruses Specifically Infecting the Marine Planktonic Diatoms
Authors: Tomaru Yuji , Kimura Kei
Published: June 2020

Since their discovery, at least 15 diatom viruses have been isolated and characterised using a culture method with two cycles of extinction dilution. However, the method is time consuming and laborious, and it isolates only the most dominant virus in a water sample. Recent studies have suggested inter-species host specificity of diatom viruses. Here, we describe a new protocol to estimate previously unrecognised host-virus relationships. Host cell cultures after inoculation of natural sediment pore water samples were obtained before complete lysis. The proliferated viral genomes in the host cells were amplified using degenerate primer pairs targeting protein replication regions of single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA) viruses, and then sequenced. Diverse ssRNA virus types within known diatom virus group were detected from inoculated Chaetoceros tenuissimus and C. setoensis cells. A previously unknown ssDNA virus type was detected in inoculated C. tenuissimus cells, but not in C. setoensis cells. Despite the possible protocol biases, for example non-specific adsorptions of virions onto the host cells, the present method helps to estimate the viruses infectious to a single host species. Further improvements to this protocol targeting the proliferated viral genomes might reveal unexpected diatom–virus ecological relationships.
Evanescent Wave Approximation for Non-Hermitian Hamiltonians
Authors: Militello Benedetto , Napoli Anna
Published: June 2020

The counterpart of the rotating wave approximation for non-Hermitian Hamiltonians is considered, which allows for the derivation of a suitable effective Hamiltonian for systems with some states undergoing decay. In the limit of very high decay rates, on the basis of this effective description we can predict the occurrence of a quantum Zeno dynamics, which is interpreted as the removal of some coupling terms and the vanishing of an operatorial pseudo-Lamb shift.
Fault Detection Algorithm for Wind Turbines’ Pitch Actuator Systems
Authors: Pujol-Vazquez Gisela , Acho Leonardo , Gibergans-Báguena José
Published: June 2020

A fault detection innovation to wind turbines’ pitch actuators is an important subject to guarantee the efficiency wind energy conversion and long lifetime operation of these rotatory machines. Therefore, a recent and effective fault detection algorithm is conceived to detect faults on wind turbine pitch actuators. This approach is based on the interval observer framework theory that has proved to be an efficient tool to measure dynamic uncertainties in dynamical systems. It is evident that almost any fault in any actuator may affect its historical-time behavior. Hence, and properly conceptualized, a fault detection system can be successfully designed based on interval observer dynamics. This is precisely our main contribution. Additionally, we realize a numerical analysis to evaluate the performance of our approach by using a dynamic model of a pitch actuator device with faults. The numerical experiments support our main contribution.
The Sensitivity of an Electro-Thermal Photovoltaic DC–DC Converter Model to the Temperature Dependence of the Electrical Variables for Reliability Analyses
Authors: Van De Sande Wieland , Ravyts Simon , Alavi Omid , Nivelle Philippe , Driesen Johan , Daenen Michaël
Published: June 2020

The operational expenditures of solar energy are gaining attention because of the continuous decrease of the capital expenditures. This creates a demand for more reliable systems to further decrease the costs. Increased reliability is often ensured by iterative use of design for reliability. The number of iterations that can take place strongly depends on the computational efficiency of this methodology. The main research objective is to quantify the influence of the temperature dependence of the electrical variables used in the electro-thermal model on the reliability and the computation time. The influence on the reliability is evaluated by using a 2-D finite elements method model of the MOSFET and calculating the plastic energy dissipation density in the die-attach and the bond wire. The trade-off between computation time of the electro-thermal model in PLECS (4.3, Plexim, Zurich, Switzerland) and generated plastic energy accuracy obtained in COMSOL (5.3, COMSOL Inc., Burlington, MA, USA) is reported when excluding a certain temperature dependence. The results indicate that the temperature dependence of the input and output capacitors causes no change in the plastic energy dissipated in the MOSFET but does introduce the largest increase in computation time. However, not including the temperature dependence of the MOSFET itself generates the largest difference in plastic energy of 10% as the losses in the die are underestimated.
Methane Oxidation Efficiency in Biofiltration Systems with Different Moisture Content Treating Diluted Landfill Gas
Authors: Frasi Niccolò , Rossi Elena , Pecorini Isabella , Iannelli Renato
Published: June 2020

This study investigates the influence of moisture content on the potential oxidation efficiency of methane (CH4) of biofiltration systems treating landfill gas containing high oxygen concentrations. Column tests filled with compost with different moisture contents (20%, 30%, and 40%) loaded with different methane flows were set up on a laboratory scale. Analyzing the results the following evidences can be summarized: With low methane load (<100 g CH4 m−2 d−1), a moisture content of 20% was not enough to support bacterial activity, while a moisture content of 40% advantaged the compost respiration assisting it to become the dominating process; with higher methane load (100–300 g CH4 m−2 d−1), a moisture content of 30% resulted in an optimal value to support methanotrophic activity showing the highest CH4 concentration reduction; moving on to a CH4 load above 300 g CH4 m−2 d−1, the inhibition of methanotrophic activity emerged independently to the moisture content of the filter media. The optimal configuration is obtained for a moisture content of 30% and in the case of flows below 200 g CH4 m−2 d−1 for which the oxidation efficiency results higher than 80%.
Performance and Design Optimization of Two-Mirror Composite Concentrating PV Systems
Authors: Li Guihua , Yu Yamei , Tang Runsheng
Published: June 2020

The reflectors of a linear solar concentrator investigated in this work consisted of two plane mirrors (2MCC), and they were designed in such a way that made all radiation within the acceptance angle (θa) arrive on flat-plate absorber, after less than two reflections. To investigate the performance of an east–west aligned 2MCC-based photovoltaic (PV) system (2MCPV), a mathematical procedure was suggested based on the three-dimensional radiation transfer and was validated by the ray-tracing analysis. Analysis indicated that the performance of 2MCPV was dependent on the geometry of 2MCC, the reflectivity of mirrors (ρ), and solar resources in a site, thus, given θa, an optimal geometry of 2MCC for maximizing the annual collectible radiation (ACR) and annual electricity generation (AEG) of 2MCPV in a site could be respectively found through iterative calculations. Calculation results showed that when the ρ was high, the optimal design of 2MCC for maximizing its geometric concentration (Cg) could be utilized for maximizing the ACR and AEG of 2MCPV. As compared to similar compound parabolic concentrator (CPC)-based PV systems, the 2MCPV with the tilt-angle of the aperture yearly fixed (1T-2MCPV), annually generated more electricity when the ρ was high; and the one with the tilt-angle adjusted yearly four times at three tilts (3T-2MCPV), performed better when θa < 25° and ρ > 0.7, even in sites with poor solar resources.
Optimal Control for Hydraulic Cylinder Tracking Displacement of Wave Energy Experimental Platform
Authors: Zhang Wei , Li Shizhen , Liu Yanjun , Li Detang , He Qin
Published: June 2020

The wave energy converter captures the wave power by buoy’s heaving motion, transfers it by hydraulic system, and converts it into the electric power by generator. The hydraulic conversion system plays an important role that can realize the effective regulation of the output power. In order to develop the working characteristics of the hydraulic transmission system, a new wave energy experimental platform was devised. The platform adopts the matching design mode of the driving oil cylinder and the driven oil cylinder. The active hydraulic cylinder and the clump weight can simulate the movement of the oscillating float under certain sea conditions, and the driven oil cylinder realizes the conversion and the output of wave energy. In order to improve the operation accuracy of the active hydraulic cylinder, the control strategy of the active hydraulic cylinder was studied. An adaptive sliding mode control strategy based on the back-stepping method was proposed to overcome the influence of the parameter uncertainty in state equation. The adaptive law was designed by Lyapunov criterion to ensure the stability and the convergence of the closed-loop system. The proposed control strategy was verified and compared with proportional integral derivative control strategy through the concrete experiment, which shows the rapidity and the stability of it. The hydraulic transmission system of wave energy converter was developed; at the same time, the characteristics of hydraulic regulation under different working conditions were summarized through experiments. The results of the research could be the guidance for the power control design.
Effect of FSI Based Ionic Liquid on High Voltage Li-Ion Batteries
Authors: Zhang Wenlin , Zhao Yongqi , Huo Yu
Published: June 2020

In this manuscript, a functionalized ionic liquid 1-cyanoethyl-2-methyl-3-allylimidazolium bis (trifluoromethanesulfonimide) salt (CEMAImTFSI) was synthesized and explored as an electrolyte component to improve the oxidation resistance of the electrolyte in high-voltage lithium-ion batteries. Based on the calculation by Gaussian 09, CEMAImTFSI has a higher highest occupied molecular orbital (HOMO) value than the organic solvents ethylene carbonate (EC) and dimethyl carbonate (DMC), suggesting that CEMAImTFSI is more susceptible to oxidation than EC and DMC. Moreover, a low Li+ binding energy value of –3.71 eV and the lower lowest unoccupied molecular orbital (LUMO) enable CEMAImTFSI to migrate easily to the surface of the LiNi0.5Mn1.5O4 cathode and participate in the formation of the SEI (solid electrolyte interphase) film, protecting the electrode materials. Electrochemical studies showed that the LiNi0.5Mn1.5O4/Li cell with 1.0 mol/L LiPF6-EC/DMC/10 vol% has the best cycling stability in the voltage range of 3–5 V. The initial discharge specific capacity of the cells was 131.03 mAh·g−1 at 0.2 C, and even after 50 cycles the discharge specific capacity value of 126.06 mAhg−1 was observed, with the cell showing a capacity retention as high as 96.2%. Even at the rate of 5 C, the average discharge specific capacity of the cell was still 109.30 mAh·g−1, which was 1.95 times higher than the cell without the CEMAImTFSI addition. The ionic liquid molecules adsorption on the cell electrode surface was confirmed by X-ray photoelectron spectroscopic (XPS) analysis after charge–discharge measurements.
A Review of the Relation between Household Indoor Temperature and Health Outcomes
Authors: Lima Fátima , Ferreira Paula , Leal Vítor
Published: June 2020

This paper provides a review of research that addresses the relationship between indoor temperatures and health outcomes, taking into consideration studies that focus heat or cold exposure within the household context. It aims to extend previous research by considering both indoor temperatures from existing housing, and empirical studies that focus on energy efficiency measures and subsequent health impacts. To achieve this aim, a literature review was undertaken, combining engineering and health databases. The review established that, overall, inadequate indoor temperatures are associated with poor health status, whereas energy efficiency measures have been associated to improved indoor temperatures and occupant’s health namely regarding cardiovascular, respiratory and mental health disorders. These health conditions are among the most prevalent non-communicable diseases (NCD). The review also highlighted the need for more empirical studies with an extended timeframe to deal with climate change challenges. It underlined the potential advantages of the convergence between health and energy efficiency studies, for better modelling and planning.
Characteristics of Flame Stability and Gaseous Emission of Bio-Crude Oil from Coffee Ground in a Pilot-Scale Spray Burner
Authors: Choi Sang Kyu , Choi Yeon Seok , Jeong Yeon Woo , Han So Young , Van Nguyen Quynh
Published: June 2020

Coffee ground has been recently considered as a new biomass resource in relation to the increasing coffee consumption worldwide. The bio-crude oil can be produced by fast pyrolysis of coffee ground, and it has advantages of larger heating values in comparison with those from other biomass. But the bio-crude oil from coffee ground has a significantly high viscosity which can hinder the application to conventional burners. In this study, a pilot-scale burner system with a 35 kW capacity with an air-blast atomizing nozzle was developed for the combustion of bio-crude oil from coffee ground with a high viscosity. A downward fuel injection system was adopted to enhance the ignition of fuel spray and the flame stabilization, and a movable block swirl generator was installed for the combustion air. The bio-crude oil was blended with ethanol at the volumetric ratio of 9:1 to enhance the combustion characteristics. The effect of various atomizing air pressures, swirl intensities, and overall equivalence ratios on the flame stability and gaseous emission were investigated to find out the optimum operating conditions for a bio-crude oil burner.
Improved Solubility Model for Pure Gas and Binary Mixture of CO2-H2S in Water: A Geothermal Case Study with Total Reinjection
Authors: H. Niknam Pouriya , Talluri Lorenzo , Fiaschi Daniele , Manfrida Giampaolo
Published: June 2020

Geothermal energy is acknowledged globally as a renewable resource, which, unlike solar, wind or wave energy, can be continuously exploited. The geothermal fluids usually have some acid gas content, which needs to be precisely taken into account when predicting the actual potential of a power plant in dealing with an effective reinjection. One of the key parameters to assess is the solubility of the acid gas, as it influences the thermodynamic conditions (saturation pressure and temperature) of the fluid. Therefore, an enhanced solubility model for the CO2-H2S-water system is developed in this study, based on the mutual solubility of gases. The model covers a wide range of pressures and temperatures. The genetic algorithm is employed to calculate the correlation constants and corresponding solubility values of both CO2 and H2S as functions of pressure, temperature and the balance of the gas. The results are validated against previously published models and experimental data available in the literature. The proposed model estimates the pure gas solubility, which is also a feature of other models. The more innovative feature of the model is the solubility estimation of each CO2 or H2S in simultaneous presence, such as when the binary gas is injected into the pure water of the geothermal reinjection well. The proposed solubility model fits well with the available experimental data, with a mean deviation lower than 0.2%.