bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Validation of a Questionnaire Evaluating the Impact of Prosthetic Dental Treatments on Patients’ Oral Health Quality of Life: A Prospective Pilot Study
Authors: Mijiritsky Eitan , Lerman Yael , Mijiritsky Ori , Shely Asaf , Meyerson Joseph , Shacham Maayan
Published: July 2020

Objectives: the aims of this study were the development of a novel questionnaire to assess the impact of prosthetic treatments on oral health-related quality of life (OHRQoL) and the performance of a prospective pilot study. Background: the currently preferred OHRQoL measurement tool is the oral health impact profile-49 (OHIP-49), a self-report questionnaire which mainly focuses on general effects related to oral health. Materials and methods: A total of 24 adult participants (9 females and 15 males) were recruited and asked to complete the novel questionnaire twice: once before the prosthetic treatment began and 4–6 weeks post-treatment. The assessment of the change in OHRQoL was based on the differences in participants’ answers before and after treatment. Data were analyzed using ANOVA with a repeated-measures method and t-tests. The reliability of the questionnaire was tested using Cronbach’s alpha and intraclass coefficient (ICC). Results: The questionnaire was found to be reliable (α ≥ 0.6), with “social disability” having the highest score (α = 0.868). All domains showed an improvement (α < 0.005) in OHRQoL scores after treatment. Conclusions: the novel questionnaire tested in this study was found to be reliable and convenient to use, and demonstrated that prosthetic treatments have a significant positive effect on OHRQoL post-treatment scores.
Feasibility and Reliability of a Questionnaire to Assess the Mode, Frequency, Distance and Time of Commuting to and from School: The PACO Study
Authors: Segura-Díaz José , Rojas-Jiménez Álvaro , Barranco-Ruiz Yaira , Murillo-Pardo Berta , Saucedo-Araujo Romina , Aranda-Balboa María , Herrador-Colmenero Manuel , Villa-González Emilio , Chillón Palma
Published: July 2020

Active commuting to and from school has several health implications. Self-reporting is the most common assessment tool, but there is a high heterogeneity of questionnaires in the scientific literature. The purpose of this study was to analyse the feasibility and reliability of the Spanish “New Version of Mode and Frequency of Commuting To and From School” questionnaire in children and adolescents. A total of 635 children (5–12 years old) and 362 adolescents (12–18 years old) filled out the questionnaire twice (14 days apart). Feasibility was evaluated using an observational checklist. The test-retest reliability of the “New Version of Mode and Frequency of Commuting To and From School” questionnaire and the distance and time to school were examined using the kappa and weight kappa coefficient (κ). No misunderstanding of questions was reported. The time to complete the questionnaire was 15 ± 3.62 and 9 ± 2.26 min for children and adolescents, respectively. The questionnaire showed substantial and almost perfect kappa coefficients for the overall six items (k = 0.61–0.94) in children and adolescents. The “New Version of Mode and Frequency of Commuting To and From School” questionnaire is a feasible and reliable questionnaire in Spanish children and adolescents.
Microbiological Hygiene Quality of Thermal Muds: A Pilot Study in Pelotherapy Facilities of the Euganean Thermal District (NE Italy)
Authors: Baldovin Tatjana , Amoruso Irene , Caldara Fabrizio , Buja Alessandra , Baldo Vincenzo , Cocchio Silvia , Bertoncello Chiara
Published: July 2020

Evaluation of hygienic aspects of thermal mud microbiology is still neglected. This study evaluates the microbiological hygiene quality of thermal muds, providing a comprehensive assessment of the whole mud cultivation chain. Maturing mud, peloid and used mud samples were collected twice in a year from 30 SPAs of the Euganean Thermal District, NE Italy. Samples were processed with an ad hoc laboratory method. The following indicator parameters were assessed: Total Count at 22, 37 and 55 °C; total coliforms; Escherichia coli; enterococci; Staphylococcus aureus; Pseudomonas aeruginosa; sulfite-reducing clostridia; dermatophytes. Statistical significance of differences between the two sampling campaigns and correlation between temperature and indicator parameters were evaluated. One-hundred eighty samples were analyzed. Widespread presence of environmental species was found, as well as hints of possible microorganism transfer from the patient’s skin to the mud. Proper setting of thermal water temperature resulted critical, in terms of hygienic quality. Although optimal maturation should be granted (thermal water at 30–42 °C), a pasteurization step at 60–65 °C is strongly recommended to sanitize peloids before pelotherapy. Facilities re-using thermal muds should also implement a regeneration step at ≥65 °C. Core evaluation of thermal mud hygienic quality could encompass the following guidelines: absence (i.e., 0 colony forming units (CFU)/g) of E. coli, P. aeruginosa, S. aureus and dermatophytes.
Addition of an Emotionally Stable Node in the SOSa-SPSa Model for Group Emotional Contagion of Panic in Public Health Emergency: Implications for Epidemic Emergency Responses
Authors: Ni Xiaoyang , Zhou Haojie , Chen Weiming
Published: July 2020

Sentiment contagion is similar to an infectious disease that spreads in a crowd. In this study, we explore the law of emotional infection under sudden public events by SIR model. The paper adds an emotionally stable node and establishes a group emotional infection model of U-SOSPa-SPSOa model. Simulation results show that our model is reasonable and can better explain the entire contagion process by considering four groups (unsusceptible-susceptible-optimistic-pessimistic) of people. Our theoretical results show: When the pessimists were below the critical value of 0.34, the number of negative emotional groups first increased and then decreased. As the proportion increases, the emotional peak of pessimists increases. The cure probability θo has the least influence on the P(t), and at the same time, under the action of θp, the P(t) reaches the stable state first. The increase of the risk coefficient can promote the pessimist infection. When the degree of risk is low, the rate of emotional infection is increased. When the degree of risk is high, the rate of infection is slowed. Therefore, system customizers and related managers can improve the efficiency of stable groups, adjust the proportion of initial negative emotions, control the infection of the spontaneous infection process, and directly deal with negative emotions. They can carry out treatment and other means to stabilize group emotions and maintain social stability.
Determination of the Microbial and Chemical Loads in Rivers from the Quito Capital Province of Ecuador (Pichincha)—A Preliminary Analysis of Microbial and Chemical Quality of the Main Rivers
Authors: Borja-Serrano Pamela , Ochoa-Herrera Valeria , Maurice Laurence , Morales Gabriela , Quilumbaqui Cristian , Tejera Eduardo , Machado António
Published: July 2020

Contamination of natural water sources is one of the main health problems worldwide, which could be caused by chemicals, metals, or microbial agents. This study aimed to analyze the quality of 18 rivers located in Quito, the capital province of Pichincha, Ecuador, through physico-chemical and microbial parameters. The E. coli and total coliforms assessments were performed by a counting procedure in growth media. Polymerase chain reaction (PCR) was realized to detect several microbial genera, as well as Candida albicans, two parasites (Cryptosporidium and Giardia spp.) and E. coli pathotypes: enterohemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC) and enteropathogenic E. coli (EPEC). Additionally, physico-chemical parameters and major and trace metals were analyzed in each surface water sample. Our results demonstrated that most of the rivers analyzed do not comply with the microbial, physico-chemical, and metal requirements established by the Ecuadorian legislation. In terms of microbial pollution, the most polluted rivers were Monjas, Machángara, Pisque, and Pita Rivers. Furthermore, three out of four analyzed E. coli pathotypes (EIEC, EHEC, and EAEC) were detected in certain rivers, specifically: Monjas River showed the presence of EIEC and EHEC; in the Machángara River, EAEC and EIEC were detected; and finally, EIEC was present in the Guayllabamba River. Several physico-chemical parameters, such as pH, CODtotal, and TSS values, were higher than the Ecuadorian guidelines in 11, 28, and 28% of the rivers, respectively. Regarding heavy metals, Zn, Cu, Ni, Pb, Cd, and Mn surpassed the established values in 94, 89, 61, 22, 22, and 17% of the rivers, respectively. Machangara River was the only one that registered higher Cr concentrations than the national guidelines. The values of Al and Fe were above the recommended values in 83 and 72% of the rivers. Overall, based on the physical-chemical and microbiological parameters the most contaminated rivers were Machángara and Monjas. This study revealed severe contaminations in Ecuadorean Rivers; further studies should evaluate the sources of contamination and their impact on public health.
The Use of Different Modes of Post-Activation Potentiation (PAP) for Enhancing Speed of the Slide-Step in Basketball Players
Authors: Gepfert Mariola , Golas Artur , Zajac Tomasz , Krzysztofik Michal
Published: July 2020

Background: This study aimed to determine whether 5% of body mass-resisted or assisted conditioning activity (CA) can enhance 5 m slide-step movement performance. Methods: Sixteen division I basketball players participated in this study (23.6 ± 4.4 years; 86.3 ± 5.9 kg; 192.3 ± 6.2 cm; training experience 6.7 ± 2.6 years). The experiment was performed following a randomized crossover design, where each participant performed two different exercise protocols—assisted and resisted CA each consisting of four sets of 10 m slide-step movement with 5% of body mass external load and 1 min rest intervals between. To assess the differences between baseline and post-assisted, as well as post-resisted CA, the participants performed a 2 × 5 m slide-step movement 6 min after each CA protocol. The differences in time between baseline, post-assisted and post-resisted CA were examined using repeated-measures ANOVA. Results: ANOVA indicated a statistically significant difference between baseline and post-assisted postactivation performance enhancement (PAPE) (p = 0.011). There were no significant intragroup differences between baseline and post-resisted CA (p = 0.230). Conclusion: Findings of the study show that a light load assisted CA (5% of body mass) effectively elicits a potentiation response among basketball players.
A System Model of Post-Migration Risk Factors Affecting the Mental Health of Unaccompanied Minor Refugees in Austria—A Multi-Step Modeling Process Involving Expert Knowledge from Science and Practice
Authors: Hynek Nicole , Franczukowska Arleta , Rössl Lydia , Schreder Günther , Faustmann Anna , Krczal Eva , Skrivanek Isabella , Sommer Isolde , Zenk Lukas
Published: July 2020

Various studies have indicated that unaccompanied minor refugees (UMRs) have a higher risk of suffering from mental health problems than do accompanied minor refugees and general population norm. However, only a few studies provide data on UMRs regarding post-migration risk factors, their interrelations, and their influence on mental health. In this study, system models of post-migration risk factors for mental health and their interactions were developed in the case of Austria. In three consecutive interactive workshops with scientists and practitioners, fuzzy-logic cognitive mapping techniques were used to integrate the experts’ knowledge. The resulting final system model consists of 11 risk factors (e.g., social contacts in the host country, housing situation, or professional health care services). The model provides a deeper insight into the complexity of interrelated direct, indirect, and reciprocal relations, as well as self-reinforcing triads. This systemic approach provides a sound basis for further investigations, taking into account the inherent complex multifactorial dependencies in this topic.
Mental Health and Work Attitudes among People Resuming Work during the COVID-19 Pandemic: A Cross-Sectional Study in China
Authors: Song Lili , Wang Yong , Li ZhengLin , Yang Ying , Li Hao
Published: July 2020

The unprecedented outbreak of the Coronavirus Disease 2019 (COVID-19) caused an economic downturn and increased the unemployment rate in China. In this context, employees face health and social economic stressors. To assess their mental health (i.e., anxiety, depression, insomnia and somatization) and work attitudes (i.e., work engagement, job satisfaction and turnover intention) as well as the associated factors, we conducted a cross-sectional study among people who resumed work after the Spring Festival holiday during the COVID-19 pandemic. The results show that the prevalence of anxiety, depression, insomnia and somatization among these people was 12.7%, 13.5%, 20.7% and 6.6%, respectively. The major risk factor for mental health was worrying about unemployment, and the main protective factors were psychological strengths (i.e., resilience and optimism). Regarding work attitudes, the percentage of people who felt more satisfied with their job (43.8%) was larger than that of those who felt less satisfied (26.9%), while the percentage of people who thought about quitting their job more frequently (15.7%) was smaller than that of those who considered it less frequently (63.2%). However, work engagement was lower than usual. Similar to the factors associated with mental health, the major risk factor for work attitudes was also worrying about unemployment, and the main protective factors were resilience and optimism. In addition, the nature of the organization, job status, age, position and income changes were also related to these work attitudes. Our findings shed light on the need for organization administrators to be aware of the status of and factors associated with employees’ mental health and work attitudes during the COVID-19 pandemic. Policies or interventions could be developed based on our findings.
The Potential Impacts of Urban and Transit Planning Scenarios for 2031 on Car Use and Active Transportation in a Metropolitan Area
Authors: Morency Patrick , Plante Céline , Dubé Anne-Sophie , Goudreau Sophie , Morency Catherine , Bourbonnais Pierre-Léo , Eluru Naveen , Tétreault Louis-François , Hatzopoulou Marianne , Iraganaboina Naveen Chandra , Bhowmik Tanmoy , Smargiassi Audrey
Published: July 2020

Land use and transportation scenarios can help evaluate the potential impacts of urban compact or transit-oriented development (TOD). Future scenarios have been based on hypothetical developments or strategic planning but both have rarely been compared. We developed scenarios for an entire metropolitan area (Montreal, Canada) based on current strategic planning documents and contrasted their potential impacts on car use and active transportation with those of hypothetical scenarios. We collected and analyzed available urban planning documents and obtained key stakeholders’ appreciation of transportation projects on their likelihood of implementation. We allocated 2006–2031 population growth according to recent trends (Business As Usual, BAU) or alternative scenarios (current planning; all in TOD areas; all in central zone). A large-scale and representative Origin-Destination Household Travel Survey was used to measure travel behavior. To estimate distances travelled by mode, in 2031, we used a mode choice model and a simpler method based on the 2008 modal share across population strata. Compared to the BAU, the scenario that allocated all the new population in already dense areas and that also included numerous public transit projects (unlikely to be implemented in 2031), was associated with greatest impacts. Nonetheless such major changes had relatively minor impacts, inducing at most a 15% reduction in distances travel by car and a 28% increase in distances walked, compared to a BAU. Strategies that directly target the reduction of car use, not considered in the scenarios assessed, may be necessary to induce substantial changes in a metropolitan area.
Clinical Presentation of COVID-19: Case Series and Review of the Literature
Authors: Macera Margherita , De Angelis Giulia , Sagnelli Caterina , Coppola Nicola ,   Vanvitelli COVID-19 Group
Published: July 2020

COVID-19 infection has a broad spectrum of severity ranging from an asymptomatic form to a severe acute respiratory syndrome that requires mechanical ventilation. Starting with the description of our case series, we evaluated the clinical presentation and evolution of COVID-19. This article is addressed particularly to physicians caring for patients with COVID-19 in their clinical practice. The intent is to identify the subjects in whom the infection is most likely to evolve and the best methods of management in the early phase of infection to determine which patients should be hospitalized and which could be monitored at home. Asymptomatic patients should be followed to evaluate the appearance of symptoms. Patients with mild symptoms lasting more than a week, and without evidence of pneumonia, can be managed at home. Patients with evidence of pulmonary involvement, especially in patients over 60 years of age, and/or with a comorbidity, and/or with the presence of severe extrapulmonary manifestations, should be admitted to a hospital for careful clinical-laboratory monitoring.
Shale Gas Development and Community Distress: Evidence from England
Authors: Aryee Feizel , Szolucha Anna , Stretesky Paul B. , Short Damien , Long Michael A. , Ritchie Liesel A. , Gill Duane A.
Published: July 2020

This research examines psychosocial stress associated with shale gas development through the narratives of residents and the Revised Impact of Event Scale (IES-R). We carried out our research in three of England’s communities impacted by shale gas development. To gather data, we conducted qualitative interviews and engaged in participant observation in all three communities and conducted a quantitative survey of residents. From our qualitative interviews it was apparent that the residents we spoke with experienced significant levels of stress associated with shale gas development in each community. Importantly, residents reported that stress was not only a reaction to development, but a consequence of interacting with industry and decision makers. Our quantitative findings suggest that a significant portion of residents 14.1% living near the shale gas sites reported high levels of stress (i.e., scoring 24 or more points) even while the mean IES-R score of residents living around the site is relatively low (i.e., 9.6; 95% CI 7.5–11.7). We conclude that the experiences, of the three English communities, reported in the qualitative interviews and quantitative survey are consistent with the reports of stress in the United States for those residents who live in shale gas communities. We therefore suggest that psychosocial stress is an important negative externality, which needs to be taken seriously by local planning officers and local planning committees when considering exploration and development permits for shale gas.
Examining the Gaps and Issues of End-of-Life Care among Older Population through the Lens of Socioecological Model—A Multi-Method Qualitative Study of Hong Kong
Authors: Chung Roger Yat-Nork , Dong Dong , Chau Nancy Nam Sze , Chau Patsy Yuen-Kwan , Yeoh Eng Kiong , Wong Eliza Lai-Yi
Published: July 2020

End-of-life (EOL) care for terminal illness and life-limiting conditions is a sector in the health service spectrum that is drawing increased attention. Despite having the world’s longest life expectancy and an ever-escalating demand for long-term care, Hong Kong’s EOL care was underdeveloped. The current study aims to provide a holistic picture of gaps and issues to EOL care in Hong Kong. Data collection was conducted using a multi-method qualitative approach that included focus groups and in-depth interviews with key informants and stakeholders, and longitudinal case studies with patients and families. Deductive thematic analysis was used to examine service gaps in current EOL care through the lens of a socioecological model where gaps and issues in various nested, hierarchical levels of care as well as the relationships between these levels were studied in detail. Using the model, we identified gaps and issues of EOL care among older populations in Hong Kong at the policy, legal, community, institutional, as well as intrapersonal and interpersonal levels. These include but are not limited to a lack of overarching EOL care policy framework, ambiguity in the legal basis for mental incapacity, legislative barriers for advance directives, inadequate capacity, resources, and support in the community to administer EOL care, inadequate knowledge, training, and resources for EOL care in health and social care sectors, inadequate medical-social interface, general reluctance and fear of death and dying, as well as the cultural interpretation of filial piety that may lengthen the suffering of the dying patients. Findings highlight the multi-level gaps and issues of EOL care in a place where western and eastern culture meet, and shed light on how best to design more effective and comprehensive policy interventions that will likely have a more sustainable and instrumental impact on facilitating person-centered EOL care during the end of life.
Spatiotemporal Characteristics and Driving Force of Ecosystem Health in an Important Ecological Function Region in China
Authors: Shen Wei , Zheng Zhicheng , Qin Yaochen , Li Yang
Published: July 2020

Quantitative assessment can scientifically determine the health status of a regional ecosystem, identify regional eco-environmental problems, and assist in promoting regional sustainable development and environmental management. Taking China’s important ecological function region, the Yellow River affected area as an example, this study constructed an extended evaluation index system based on the pressure-state-response framework, and remote sensing and GIS techniques were used to dynamically evaluate the spatial and temporal characteristics of ecosystem health in the study area. Furthermore, influencing factors on ecosystem health in the study area were extensively analyzed using the GeoDetector model. The results show that the ecosystem health level in the study area shows significant spatial heterogeneity from 1995–2015, and showed a fluctuating change process. Areas with large fluctuations in health level were mainly distributed in extreme climate areas, ecologically fragile areas, on plains and in hilly areas. Spatial differences of ecosystem health were well explained by using the biological abundance index, relief degree of land surface, soil type, annual average precipitation, elevation, annual average temperature, and population density. Influencing factors have significant interactive effects on ecosystem health.
The Roses Ocean and Human Health Chair: A New Way to Engage the Public in Oceans and Human Health Challenges
Authors: Lloret Josep , Abós-Herràndiz Rafael , Alemany Sílvia , Allué Rosario , Bartra Joan , Basagaña Maria , Berdalet Elisa , Campàs Mònica , Carreño Arnau , Demestre Montserrat , Diogène Jorge , Fontdecaba Eva , Gascon Mireia , Gómez Sílvia , Izquierdo Angel , Mas Lluïsa , Marquès Montse , Pedro-Botet Juan , Pery Maria , Peters Francesc , Pintó Xavier , Planas Marta , Sabatés Ana , San Joan , Sanchez-Vidal Anna , Trepat Martí , Vendrell Cristina , Fleming Lora E.
Published: July 2020

Involving and engaging stakeholders is crucial for studying and managing the complex interactions between marine ecosystems and human health and wellbeing. The Oceans and Human Health Chair was founded in the town of Roses (Catalonia, Spain, NW Mediterranean) in 2018, the fruit of a regional partnership between various stakeholders, and for the purpose of leading the way to better health and wellbeing through ocean research and conservation. The Chair is located in an area of the Mediterranean with a notable fishing, tourist, and seafaring tradition and is close to a marine reserve, providing the opportunity to observe diverse environmental conditions and coastal and maritime activities. The Chair is a case study demonstrating that local, collaborative, transdisciplinary, trans-sector, and bottom-up approaches offer tremendous opportunities for engaging coastal communities to help support long-lasting solutions that benefit everyone, and especially those living by the sea or making their living from the goods and services provided by the sea. Furthermore, the Chair has successfully integrated most of its experts in oceans and human health from the most prestigious institutions in Catalonia. The Chair focuses on three main topics identified by local stakeholders: Fish and Health; Leisure, Health, and Wellbeing; and Medicines from the Sea. Led by stakeholder engagement, the Chair can serve as a novel approach within the oceans and human health field of study to tackle a variety of environmental and public health challenges related to both communicable and non-communicable diseases, within the context of sociocultural issues. Drawing on the example provided by the Chair, four principles are established to encourage improved participatory processes in the oceans and human health field: bottom-up, “think local”, transdisciplinary and trans-sectorial, and “balance the many voices”.
An Epidemiological Study on the Prevalence of the Clinical Features of SARS-CoV-2 Infection in Romanian People
Authors: Ciucurel Constantin , Iconaru Elena Ioana
Published: July 2020

The aim of this study was to investigate the prevalence of the clinical features of the SARS-CoV-2 infection in Romanian population through a novel online survey. The survey included categorical socio-demographic and health-related variables. A total of 1830 participants were selected for statistical data processing (a response rate of 90.9%). We determined reasonable reliability of the survey section for clinical features of SARS-CoV-2 infection (Cronbach’s Alpha 0.671). Two meaningful dimensions were identified through CATPCA (Categorical Principal Component Analysis) for the survey’s items. We separated two significant clusters of items, each measuring a distinct factor: the sociodemographic characteristics linked to social distancing and the relevant clinical features of SARS-CoV-2 infection. Next, a two-step cluster analysis helped to classify the sample group taking into consideration the similarity of subjects. The clustering revealed a three-cluster solution, with significant differences between clusters and allowed the cluster detection of a group of individuals, possibly more affected by the infection with the SARS-CoV-2 virus. Through binomial logistic regression analysis, we identified a statistically significant prediction model for the presumptive diagnostic of some relevant clinical features of SARS-CoV-2 infection. Our study validated a cost-effective model for rapid assessment of the health status of subjects, adapted to the context of SARS-CoV-2 pandemic.
Dietary Diversity Score: Implications for Obesity Prevention and Nutrient Adequacy in Renal Transplant Recipients
Authors: Lin I-Hsin , Duong Tuyen Van , Nien Shih-Wei , Tseng I-Hsin , Wang Hsu-Han , Chiang Yang-Jen , Chen Chia-Yen , Wong Te-Chih
Published: July 2020

Obesity affects both medical and surgical outcomes in renal transplant recipients (RTRs). Dietary diversity, an important component of a healthy diet, might be a useful nutritional strategy for monitoring patients with obesity. In this cross-sectional study, the data of 85 eligible RTRs were analyzed. Demographic data, routine laboratory data, and 3-day dietary data were collected. Participants were grouped into nonobesity and obesity groups based on body mass index (BMI) (for Asian adults, the cutoff point is 27 kg/m2). Dietary diversity score (DDS) was computed by estimating scores for the six food groups emphasized in the Food Guide. The mean age and BMI of participants were 49.7 ± 12.6 years and 24.0 ± 3.8 kg/m2, respectively. In the study population, 20.0% (n = 17) were obese. DDS was significantly lower in obese participants than in those who were not obese (1.53 ± 0.87 vs. 2.13 ± 0.98; p = 0.029). In addition, DDS was correlated with nutrition adequacy of the diet. Multivariate analysis showed that the odds of obesity decreased with each unit increase in DDS (odds ratio, 0.278; 95% confidence interval, 0.101–0.766; p = 0.013). We conclude that patients with higher dietary diversity have a lower prevalence of obesity.
Can Green Walls Reduce Outdoor Ambient Particulate Matter, Noise Pollution and Temperature?
Authors: Paull Naomi , Krix Daniel , Torpy Fraser , Irga Peter
Published: July 2020

Green walls have previously demonstrated the capacity to reduce particulate matter (PM), noise pollution, and temperature conditions in manipulative experiments and computational models. There is, however, minimal evidence that green walls can influence ambient environmental conditions, especially taking into account the variable environmental conditions encountered in situ. The aim of this paper was to determine if green walls have a quantitative effect on ambient air quality in an urban environment. Ambient PM, noise, and temperature were recorded at 12 green wall and adjacent reference wall locations across a dense urban centre, over a 6-month period. The results indicated that PM levels and temperature did not significantly differ between the green wall and reference wall sites. Ambient noise at the green wall sites, however, was significantly lower than at the reference wall locations. It is suggested that mechanically assisted, or ‘active’ green wall systems may have a higher PM and temperature reduction capacity, and if so, they will be more valuable for installation in situ compared to standard passive systems, although this will require further research.
Geospatial Virtual Reality for Cyberlearning in the Field of Topographic Surveying: Moving Towards a Cost-Effective Mobile Solution
Authors: Levin Eugene , Shults Roman , Habibi Reza , An Zhongming , Roland William
Published: July 2020

In spite of the tremendous success in artificial intelligence technology and a high level of automation in geospatial data obtaining processes, there is still a need for topographical field data collection by professional surveyors. Understanding terrain topology and topography is a cognitive skill set that has to be demonstrated by geospatial Subject Matter Experts (SME) for the productive work in the topographic surveying field. For training of the mentioned above skillset, one has to be exposed to the theory and must also practice with surveying instruments in field conditions. The challenge of any surveying/geospatial engineering workforce training is to expose students to field conditions which might be limited due to equipment expenses and meteorological conditions that prevent good data collection. To meet this challenge, the Integrated Geospatial Technology research group is working on a geospatial virtual reality (VR) project which encompasses the following components: (a) immersive visualization of terrain; (b) virtual total station instrument; (c) virtual surveyor with reflector installed on the virtual rod. The application scenario of the technology we are working with has the following stages: (1) student is installing total station on the optimal location; (2) students move virtual surveyor on the sampling points they consider to be important (3) contours are generated and displayed in 3D being superimposed on 3D terrain; (4) accuracy of terrain modeling is observable and measurable by comparing the sampling model with initial one.
Geo-Spatial Analysis in Hydrology
Authors: Zhou Qiming , Li Jianfeng
Published: July 2020

With the increasing demand for accurate and reliable hydrological information, geo-spatial analysis plays a more and more important role in hydrological studies. The development of the geo-spatial technique advances our understanding of the complex and spatially heterogeneous hydrological systems. Meanwhile, how to efficiently and effectively process and analyze multi-source geo-spatial data has become more challenging in the fields of hydrology. In this editorial, we first review the development and application of geo-spatial analysis in three major topics in hydrological studies, namely the scaling issue, extraction of basin characteristics, and hydrological modelling. We hence introduce the articles of the Special Issue. These studies present the latest results of geo-spatial analysis in different topics in hydrology, and improve geo-spatial analytic methods for better accuracy and reliability.
Advances in Understanding the Initial Steps of Pruritoceptive Itch: How the Itch Hits the Switch
Authors: Kahremany Shirin , Hofmann Lukas , Gruzman Arie , Cohen Guy
Published: July 2020

Pruritoceptive (dermal) itch was long considered an accompanying symptom of diseases, a side effect of drug applications, or a temporary sensation induced by invading pruritogens, as produced by the stinging nettle. Due to extensive research in recent years, it was possible to provide detailed insights into the mechanism of itch mediation and modulation. Hence, it became apparent that pruritus is a complex symptom or disease in itself, which requires particular attention to improve patients’ health. Here, we summarize recent findings in pruritoceptive itch, including how this sensation is triggered and modulated by diverse endogenous and exogenous pruritogens and their receptors. A differentiation between mediating pruritogen and modulating pruritogen seems to be of great advantage to understand and decipher the molecular mechanism of itch perception. Only a comprehensive view on itch sensation will provide a solid basis for targeting this long-neglected adverse sensation accompanying numerous diseases and many drug side effects. Finally, we identify critical aspects of itch perception that require future investigation.
Genetic Markers for Later Remission in Response to Early Improvement of Antidepressants
Authors: Kang Hee-Ju , Kim Ki-Tae , Yoo Kyung-Hun , Park Yoomi , Kim Ju-Wan , Kim Sung-Wan , Shin Il-Seon , Kim Ju Han , Kim Jae-Min
Published: July 2020

Planning subsequent treatment strategies based on early responses rather than waiting for delayed antidepressant action can be helpful. We identified genetic markers for later non-remission in patients exhibiting poor early improvement using whole-exome sequencing data of depressive patients treated in a naturalistic manner. Among 1000 patients, early improvement at 2 weeks (reduction in Hamilton Depression Rating Scale [HAM-D] score ≥ 20%) and remission at 12 weeks (HAM-D score ≤ 7) were evaluated. Gene- and variant-level analyses were conducted to compare patients who did not exhibit early improvement and did not eventually achieve remission (n = 126) with those who exhibited early improvement and achieved remission (n = 385). Genes predicting final non-remission in patients who exhibited poor early improvement (COMT, PRNP, BRPF3, SLC25A40, and CGREF1 in males; PPFIBPI, LZTS3, MEPCE, MAP1A, and PFAS in females; ST3GAL5 in the total population) were determined. Among the significant genes, variants in the PRNP (rs1800014), COMT (rs6267), BRPF3 (rs200565609), and SLC25A40 genes (rs3213633) were identified. However, interpretations should be made cautiously, as complex pharmacotherapy involves various genes and pathways. Early detection of poor early improvement and final non-remission based on genetic risk would be helpful for decision-making in a clinical setting.
The Role of Micro RNA and Long-Non-Coding RNA in Osteoporosis
Authors: Ko Nai-Yu , Chen Li-Ru , Chen Kuo-Hu
Published: July 2020

Osteoporosis is a major concern worldwide and can be attributed to an imbalance between osteoblastic bone formation and osteoclastic bone resorption due to the natural aging process. Heritable factors account for 60–80% of optimal bone mineralization; however, the finer details of pathogenesis remain to be elucidated. Micro RNA (miRNA) and long-non-coding RNA (lncRNA) are two targets that have recently come into the spotlight due to their ability to control gene expression at the post-transcriptional level and provide epigenetic modification. miRNAs are a class of non-coding RNAs that are approximately 18–25 nucleotides long. It is thought that up to 60% of human protein-coding genes may be regulated by miRNAs. They have been found to regulate gene expression that controls osteoblast-dependent bone formation and osteoclast-related bone remodeling. lncRNAs are highly structured RNA transcripts longer than 200 nucleotides that do not translate into proteins. They have very complex secondary and tertiary structures and the same degradation processes as messenger RNAs. The fact that they have a rapid turnover is due to their sponge function in binding the miRNAs that lead to a degradation of the lncRNA itself. They can act as signaling, decoy, and framework molecules, or as primers. Current evidence suggests that lncRNAs can act as chromatin and transcriptional as well as post-transcriptional regulators. With regards to osteoporosis, lncRNA is thought to be involved in the proliferation, apoptosis, and inflammatory response of the bone. This review, which is based on a systematic appraisal of the current literature, provides current molecular and genetic opinions on the roles of miRNAs and lncRNAs in osteoporosis. Further research into the epigenetic modification and the regulatory roles of these molecules will bring us closer to potential disease-modifying treatment for osteoporosis. However, more issues regarding the detailed actions of miRNAs and lncRNAs in osteoporosis remain unknown and controversial and warrant future investigation.
Approaching Sex Differences in Cardiovascular Non-Coding RNA Research
Authors: Jusic Amela , Salgado-Somoza Antonio , Paes Ana B. , Stefanizzi Francesca Maria , Martínez-Alarcón Núria , Pinet Florence , Martelli Fabio , Devaux Yvan , Robinson Emma Louise , Novella Susana
Published: July 2020

Cardiovascular disease (CVD) is the biggest cause of sickness and mortality worldwide in both males and females. Clinical statistics demonstrate clear sex differences in risk, prevalence, mortality rates, and response to treatment for different entities of CVD. The reason for this remains poorly understood. Non-coding RNAs (ncRNAs) are emerging as key mediators and biomarkers of CVD. Similarly, current knowledge on differential regulation, expression, and pathology-associated function of ncRNAs between sexes is minimal. Here, we provide a state-of-the-art overview of what is known on sex differences in ncRNA research in CVD as well as discussing the contributing biological factors to this sex dimorphism including genetic and epigenetic factors and sex hormone regulation of transcription. We then focus on the experimental models of CVD and their use in translational ncRNA research in the cardiovascular field. In particular, we want to highlight the importance of considering sex of the cellular and pre-clinical models in clinical studies in ncRNA research and to carefully consider the appropriate experimental models most applicable to human patient populations. Moreover, we aim to identify sex-specific targets for treatment and diagnosis for the biggest socioeconomic health problem globally.
Intracellular Fate and Impact on Gene Expression of Doxorubicin/Cyclodextrin-Graphene Nanomaterials at Sub-Toxic Concentration
Authors: Caccamo Daniela , Currò Monica , Ientile Riccardo , Verderio Elisabetta AM , Scala Angela , Mazzaglia Antonino , Pennisi Rosamaria , Musarra-Pizzo Maria , Zagami Roberto , Neri Giulia , Rosmini Consolato , Potara Monica , Focsan Monica , Astilean Simion , Piperno Anna , Sciortino Maria Teresa
Published: July 2020

The graphene road in nanomedicine still seems very long and winding because the current knowledge about graphene/cell interactions and the safety issues are not yet sufficiently clarified. Specifically, the impact of graphene exposure on gene expression is a largely unexplored concern. Herein, we investigated the intracellular fate of graphene (G) decorated with cyclodextrins (CD) and loaded with doxorubicin (DOX) and the modulation of genes involved in cancer-associated canonical pathways. Intracellular fate of GCD@DOX, tracked by FLIM, Raman mapping and fluorescence microscopy, evidenced the efficient cellular uptake of GCD@DOX and the presence of DOX in the nucleus, without graphene carrier. The NanoString nCounter™ platform provided evidence for 34 (out of 700) differentially expressed cancer-related genes in HEp-2 cells treated with GCD@DOX (25 µg/mL) compared with untreated cells. Cells treated with GCD alone (25 µg/mL) showed modification for 16 genes. Overall, 14 common genes were differentially expressed in both GCD and GCD@DOX treated cells and 4 of these genes with an opposite trend. The modification of cancer related genes also at sub-cytotoxic G concentration should be taken in consideration for the rational design of safe and effective G-based drug/gene delivery systems. The reliable advantages provided by NanoString® technology, such as sensibility and the direct RNA measurements, could be the cornerstone in this field.
Gram-Negative Bacterial Endotoxin LPS Induces NeuGc Loss through Ets1-Dependent Downregulation of Intestine-Specific pcmah Transcript in Porcine Intestinal Cells
Authors: Kwak Choong-Hwan , Song Kwon-Ho , Kim Cheorl-Ho
Published: July 2020

N-glycolylneuraminic acid (NeuGc), a non-human sialic acid derivative synthesized by cytidine-5′-monophospho-N-acetylneuraminic acid hydroxylase (CMAH), plays a crucial role in mediating infections by certain pathogens. Although it has been postulated that NeuGc biosynthesis and CMAH expression are downregulated during microbial infection, the underlying mechanisms remain unclear. The present study showed that exposure to lipopolysaccharide (LPS), a Gram-negative bacterial endotoxin, leads to loss of NeuGc biosynthesis in pig small intestinal I2I-2I cells. This LPS-induced NeuGc loss was accompanied by decreased CMAH transcript levels, especially intestine-specific 5′pcmah-1. Furthermore, LPS suppressed the activity of the Pi promoter responsible for 5′pcmah-1 by inhibiting DNA binding of Est1. These findings provide insight into the regulatory mechanisms of Neu5Gc biosynthesis during pathogenic infectious events, which may represent a host defense mechanism that protects the self against pathogenic bacterial infections even in non-sanitary environments.
Effects of Short-Chain Fatty Acids on Human Oral Epithelial Cells and the Potential Impact on Periodontal Disease: A Systematic Review of In Vitro Studies
Authors: Magrin Gabriel Leonardo , Strauss Franz Josef , Benfatti Cesar Augusto Magalhães , Maia Lucianne Cople , Gruber Reinhard
Published: July 2020

Short-chain fatty acids (SCFA), bacterial metabolites released from dental biofilm, are supposed to target the oral epithelium. There is, however, no consensus on how SCFA affect the oral epithelial cells. The objective of the present study was to systematically review the available in vitro evidence of the impact of SCFA on human oral epithelial cells in the context of periodontal disease. A comprehensive electronic search using five databases along with a grey literature search was performed. In vitro studies that evaluated the effects of SCFA on human oral epithelial cells were eligible for inclusion. Risk of bias was assessed by the University of Bristol’s tool for assessing risk of bias in cell culture studies. Certainty in cumulative evidence was evaluated using GRADE criteria (grading of recommendations assessment, development, and evaluation). Of 3591 records identified, 10 were eligible for inclusion. A meta-analysis was not possible due to the heterogeneity between the studies. The risk of bias across the studies was considered “serious” due to the presence of methodological biases. Despite these limitations, this review showed that SCFA negatively affect the viability of oral epithelial cells by activating a series of cellular events that includes apoptosis, autophagy, and pyroptosis. SCFA impair the integrity and presumably the transmigration of leucocytes through the epithelial layer by changing junctional and adhesion protein expression, respectively. SCFA also affect the expression of chemokines and cytokines in oral epithelial cells. Future research needs to identify the underlying signaling cascades and to translate the in vitro findings into preclinical models.
The Soluble Adenylyl Cyclase Inhibitor LRE1 Prevents Hepatic Ischemia/Reperfusion Damage Through Improvement of Mitochondrial Function
Authors: Teodoro João S. , Amorim João A. , Machado Ivo F. , Castela Ana C. , Steegborn Clemens , Sinclair David A. , Rolo Anabela P. , Palmeira Carlos M.
Published: July 2020

Hepatic ischemia/reperfusion (I/R) injury is a leading cause of organ dysfunction and failure in numerous pathological and surgical settings. At the core of this issue lies mitochondrial dysfunction. Hence, strategies that prime mitochondria towards damage resilience might prove applicable in a clinical setting. A promising approach has been to induce a mitohormetic response, removing less capable organelles, and replacing them with more competent ones, in preparation for an insult. Recently, a soluble form of adenylyl cyclase (sAC) has been shown to exist within mitochondria, the activation of which improved mitochondrial function. Here, we sought to understand if inhibiting mitochondrial sAC would elicit mitohormesis and protect the liver from I/R injury. Wistar male rats were pretreated with LRE1, a specific sAC inhibitor, prior to the induction of hepatic I/R injury, after which mitochondria were collected and their metabolic function was assessed. We find LRE1 to be an effective inducer of a mitohormetic response based on all parameters tested, a phenomenon that appears to require the activity of the NAD+-dependent sirtuin deacylase (SirT3) and the subsequent deacetylation of mitochondrial proteins. We conclude that LRE1 pretreatment leads to a mitohormetic response that protects mitochondrial function during I/R injury.
Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-κB and AP-1 Activities In Vitro
Authors: Peron Gregorio , Hošek Jan , Prasad Phuyal Ganga , Raj Kandel Dharma , Adhikari Rameshwar , Dall’Acqua Stefano
Published: July 2020

Here we report the comprehensive characterization of the secondary metabolites from the leaves of Colebrookea oppositifolia Smith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MSn and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-κB and AP-1 inhibitory activity of C. oppositifolia extract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate that C. oppositifolia leaves extract could significantly reduce the viability of THP-1 cells (IC50 = 6.2 ± 1.2 µg/mL), as well as the activation of both NF-κB and AP-1 at the concentration of 2 μg/mL. Our results indicate that Nepalese C. oppositifolia is a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses of C. oppositifolia in Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.