bluuebunny/crossref_metadata_2025 huggingface dataset:
METABODY AS PERFORMANCE IN DON DELILLO’S THE BODY ARTIST, 2001
Authors: Rykova Hanna
Published: December 2023

The article delves into Don DeLillo’s novel “The Body Artist” (2001), presenting it as a pioneering work that introduces a novel perspective on the concept of the body in literature. Central to the analysis is the notion of the meta-body, characterized by its dynamic and flexible nature. The study posits that the portrayal of the body undergoes a transformation, evolving from a static entity in the reader’s consciousness to a dynamic force responsive to shifts in perception and newly acquired information. The meta-body is portrayed as a fresh phenomenon, its understanding facilitated through the rejection of biases and the evaluation of the likelihood of sensations in specific contexts.A pivotal aspect of the exploration is the meta-body’s emancipation from traditional stereotypes and assumptions. It is underscored that the meta-body increasingly relies on the reader’s capacity to assess the probability of sensations in diverse circumstances, challenging preconceived notions and enriching the literary portrayal of the body as an adaptive entity within contemporary discourse.The article further scrutinizes the concept of the meta-body from various analytical perspectives within the novel. Firstly, it examines the meta-body through the collapse of distance, elucidating how spatiotemporal experiences are altered. The second perspective investigates the meta-body through the lens of material artifacts, encompassing mechanical devices such as dictaphones, radios, and webcams. Additionally, the communication-dialogical nature of family relationships is explored, emphasizing the interplay between silence and sound, verbalized and unspoken dialogues among members of a marital couple.Noteworthy is the analysis of the meta-body through the perspective of bodily performance.In summary, the article positions “The Body Artist” as a literary milestone that introduces the concept of the meta-body, portraying the body as a dynamic and adaptive entity. It goes beyond conventional literary portrayals, encouraging readers to reassess their preconceptions and engage with the body in diverse contexts, offering a nuanced understanding of its multifaceted nature within contemporary literature.
Examining the Relationship Between the Dominant Eye and the Dominant Hand
Authors:   Department of Optometry, School of Rehabilitation Sciences, Iran University of Medical Sciences, Tehran, Iran., Almasi Mohammad , Mirzajani Ali ,   Department of Optometry, School of Rehabilitation Sciences, Iran University of Medical Sciences, Tehran, Iran., Abolghasemi Jamile ,   Department of Biostatistics, School of Health, Iran University of Medical Sciences, Tehran, Iran., Rahmani Saeed ,   Department of Optometry, School of Rehabilitation Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Published: December 2023

Background and Objectives: This study aims to determine the relationship between hand dominance and ocular dominance (OD) using different test formats of handedness and ocular dominance. Methods: Three OD test formats were administered to 74 healthy young adult subjects, hole-in-card test for sighting dominance, near-point-of-convergence (NPC) test for motor dominance, and +1.50 to blur test for sensory dominance. All of these tests were performed while the best correction was in front of the subject’s eyes. An interview for preferred dominance and a finger-tapping test for performance dominance were administered to determine the dominant hand. Results: Right eye dominance was indicated in 66.2% with the hole-in-card test, 56.8% with the NPC test, and 51.4% of cases with the +1.50-to-blur test. Statistically, a significant nonrandom relationship was observed between the results of hole-in-card and NPC tests (P=0.000), hole-in-card and +1.50-to-blur tests (P=0.003), and also between the results of NPC and +1.50-to-blur tests (P=0.003). In addition, it was found that in 52.7% of the cases, all three dimensions of eye dominance (sighting, sensory, and motor) are not in the same eye and do not have pure eye dominance. In examining the dominant hand, the majority of subjects were right-handed both in the preference (62 of 74 [83.8%]) and in the performance dimension (63 of 74 [85.1%]). Statistical analysis shows a significant nonrandom relationship between the pure dominant hand and the pure dominant eye (P=0.000). In addition, the pure dominant hand (0.813) and pure dominant eye (P=0.126) are independent of gender. Conclusion: Determining the OD, similar to determining the hand dominance based on their different dimensions, can have different results. According to the results, in all dimensions of ocular and hand dominance, both the dominant eye and the dominant hand are more on the right side of people. Furthermore, the pure dominant eye and pure dominant hand are not independent of each other.
Influence of meteorological conditions of the year on the biochemical composition of sorghum grain
Authors: Bychkova V. V. , Kibalnik O. P. , Sazonova I. A. , Kameneva O. B.
Published: December 2023

Relevance. Currently, the study of the interaction of these traits with each other is a very important task of breeding. In the work, 15 varieties of grain sorghum were studied, differing in morphological parameters, growing season, degree of grain ripening, biochemical composition and yield of grain and green mass. The purpose of the study is to study the interaction of environmental conditions on the yield and biochemical composition of sorghum grain and to assess the significance of these relationships.Methods. The studies were conducted in 2020, 2021 and 2022. The years of research differed in meteorological conditions (hydrothermal Selyaninov coefficient (SCC) was 0.56–0.76). To assess the biochemical composition in sorghum grain, the spectroscopy method was used using an infrared analyzer Spectra Star XT. The grain yield was determined according to the generally accepted method. Results. The experimental results were processed using the “Agros 2.09” software package by the method of correlation analysis. Based on the results of three years of research, a correlation was established between biochemical composition and the meteorological conditions of the year (sum of temperatures, total precipitation, SCC), as well as a significant effect of these traits on the yield of sorghum grain.
Makna Sufistik Tanda-Tanda Kata Isim (Nomina) Pada Ilmu Nahwu Dalam Kitab Alfiyah Ibnu Malik (Analisis Semiotika Roland Barthes)
Authors: Zarkasyi Muhamad Qustulani
Published: December 2023

This paper discusses (1) Roland Barthes' semiotic theory; and (2) its application in finding the Sufistic meaning of the signs of the word ism (noun) in nahwu science in the Alfiyah book by Ibn Malik. This research is a qualitative descriptive literature (library) with Roland Barthes's semiotic approach, in which the text is considered autonomous from its author (the dead of author), and the meaning is divided into how many levels. Therefore, this study concludes that (1) Roland Barthes' semiotic theory opens opportunities for multilevel meanings, where the first level is called denotation, and the second level is called connotation. The meaning of each is influenced by the imagination and feelings as well as socio-historical and mythological readers; and (2) the autonomization of the text makes the meaning which originally meant "nahwu" change into the meaning of tasawuf, which is a way of achieving privilege before Allah by applying the signs of ism, namely by (1) jarr (religious/ humble). ); (2) tanwi>n (having a strong desire, strong intention and consistent value in obedience); (3) nida>' (consistent recitation/remembrance of Allah physically and mentally); (4) alif + lam "al" (thinking positively and managing the heart from negative influences and gaining knowledge; and (5) musnad ilah (real charity and establishing good relations with fellow human beings, the universe, and the creator).
„Geomorfologija“ u Istriotskom lingvističkom atlasu i dijalektalnim rječnicima Istre
Authors: Giudici Alberto
Published: December 2023

Istriotski lingvistički atlas (ILA) prvi je dio projekta Atlas Linguarum Histriae et Liburniae (Lingvistički atlas Istre i Kvarnera). Svezak koji smo ovom prilikom analizirali izdan je u Puli 1998. godine, nakon prikupljanja i analize građe koja je trajala desetak godina. Drugo izdanje Atlasa (popravljeno i dopunjeno) izašlo je 2017. Ovaj rad analize istriotskih govora razmatra semantičku skupinu „geomorfologije”, drugu u knjizi, koja sadrži trideset devet pitanja postavljenih informatorima. Glavni cilj ovoga rada bit će rasprava geografskih naziva ILA-e usredotočena na istraživanje zajedničkih karakteristika sviju šest govora, ističući gdje su prisutni, raščlanjene odgovore i usporedbu s dijalektalnim rječnicima ovoga područja. Nadalje, pokušat ćemo objasniti romanskoslavenske prožetosti ovoga pograničnog područja.
INNOVATION THROUGH PARTNERSHIP – THE IMPACT OF SRM ON PRODUCT AND SERVICE DEVELOPMENT
Authors:   Lviv Polytechnic National University, Bilyk Iryna , Klimchuk Oleksandr ,   Lviv Polytechnic National University
Published: December 2023

The article examines the key role of strategic supplier relationship management (SRM) in the modern business environment and its impact on the development of enterprise products and services. In the process of research, the method of systematic analysis of scientific sources and literature was used to assess the impact of strategic management of relationships with suppliers on the development of products and services of enterprises. In addition, a problem-oriented approach, abstract logical methods, as well as methods of analysis, synthesis, and generalization were used. SRM is a systematic approach to supplier relationship management aimed at optimizing collaboration, reducing risk and creating value for businesses. In the context of this article, various aspects of SRM are reviewed, and the focus is on tools and software that help businesses effectively manage supplier relationships. The choice of SRM tools requires a careful assessment of the needs and resources of the enterprise to achieve strategic goals and objectives. SRM plays an important role in reducing research and development (R&D) costs and accelerating the introduction of new products to market. SRM tools and programs contribute to the optimization of the supply chain by helping to select the best suppliers for the implementation of R&D projects. Important aspects of SRM effectiveness are examined, including the role of effective communication in achieving innovation and creating value for enterprises. The impact of SRM on the competitiveness of enterprises that strive to achieve competitive advantages in the modern business environment is studied. In particular, the article examines SRM tools and programs, their advantages and disadvantages, as well as the specifics of their use in various industries and types of economic activity. Based on the analysis of theoretical concepts and practical studies, strategic opportunities and challenges related to the implementation of SRM, as well as ways to overcome these challenges, were identified. In conclusion, this study reveals the importance of strategic supplier relationship management in the context of enterprise product and service development, providing insights and methods that help improve competitiveness and drive innovation.
Exploring the Relationship Between Oil Prices and Economic Growth in Türkiye
Authors: YILDIRIM Abdulmecit , KONAT Gökhan
Published: December 2023

This study investigates the existence of a long-run relationship between economic growth and oil prices in Türkiye, using annual data for the period 1987-2019. First, the stationarity of the variables is evaluated using the Residual Augmented Least Squares (RALS) based ADF unit root test. Second, the RALS-based cointegration test is used to investigate the long-run relationship between the variables. The findings indicate that there is no long-run relationship between economic growth and oil prices in Türkiye. As a result, it is concluded that the series does not return to equilibrium in the long run and oil price shocks during the financial turmoil may affect economic growth.
Subfoveal Nodule Affecting Visual Prognosis in Coats Disease
Authors: Jeong Hyun Chul , Park Sang Jun , Joo Kwangsic
Published: December 2023

Purpose: To investigate the characteristics of subfoveal nodules in Korean patients with Coats disease and their association with visual outcomes Methods: A retrospective analysis was conducted within the medical records of patients with stage 2B or 3A1 Coats disease, including clinical features, imaging, presence of either a subfoveal nodule or macular fibrosis, and visual outcome.Results: Twelve patients were present with stage 2B or 3A1 Coats disease, and nine patients (75%) presented with subfoveal nodule. Between the group without subfoveal nodule and the group with subfoveal nodule, there were no significant differences in age (mean, 14.0 ± 1.7 years vs. 27.7 ± 21.8 years; p = 0.482), sex (all men), stage of the disease (stage 2B: three patients vs. eight patients, p > 0.999; stage 3A1: none vs. one patient, p > 0.999), extension of retinal exudation (mean, 7.7 hours vs. 4.1 hours; p = 0.209) and peripheral telangiectasia (mean, 3.7 hours vs. 4.2 hours; p = 0.727), and follow-up duration (mean, 65.0 months vs. 46.1 months; p = 0.600). There were significantly more patients with severe visual loss (≤20 / 200) among the patients with subfoveal nodule (none vs. seven patients, p = 0.045), and the cause for severe visual loss was macular fibrosis in all cases. Macular fibrosis developed significantly more frequently in the patients with subfoveal nodule (none vs. seven = patients, p = 0.045).Conclusions: This study is the first study covering the analysis of subfoveal nodules in Korean patients with Coats disease. The existence of a subfoveal nodule at the initial diagnosis serves as an indicator predicting the development of macular fibrosis and a less favorable visual outcome in the patients with Coats disease. A multicenter study with a larger patient pool and further studies toward the therapeutic approach for the subfoveal nodule and macular fibrosis are needed.
SELF-MANAGEMENT DIET AND RANDOM PLASMA GLUCOSE CONTROL OF PATIENT WITH TYPE 2 DIABETES MELLITUS AT PUSKESMAS ALUN-ALUN GRESIK
Authors:   Widya Mandala Surabaya Catholic University, Willianto Vincentius Michael , Cundawan Robertus Saldi ,   Widya Mandala Surabaya Catholic University, Julius Dana Parama ,   Widya Mandala Surabaya Catholic University, Ananta I Dewa Gede Pangestu Banu ,   Widya Mandala Surabaya Catholic University, Hilarius Sancha Melarisa ,   Widya Mandala Surabaya Catholic University, Setiawan Christin ,   Widya Mandala Surabaya Catholic University, Khorianto Alecia Fransiska ,   Widya Mandala Surabaya Catholic University, Grecia Valerie ,   Widya Mandala Surabaya Catholic University, Dayoh Paul Jhosua ,   Widya Mandala Surabaya Catholic University, Dwiranti Jasinda ,   Widya Mandala Surabaya Catholic University, Murbarani Ivita Tahta ,   Widya Mandala Surabaya Catholic University, Vincensa Elicia , ,   Widya Mandala Surabaya Catholic University, Taufik Ahmad ,   Puskesmas Alun-Alun Gresik, Rihadi Lukas Slamet ,   Widya Mandala Surabaya Catholic University, Wattimena Inge ,   Widya Mandala Surabaya Catholic University, Sustini Florentina ,   Widya Mandala Surabaya Catholic University, Wijono Steven , ,   Widya Mandala Surabaya Catholic University, Dewi Dewa Ayu Liona ,   Widya Mandala Surabaya Catholic University, Sincihu Yudhiakuari ,   Widya Mandala Surabaya Catholic University
Published: December 2023

Background: Diabetes Mellitus (DM) is a non-communicable metabolic disease characterized by the pancreas not being able to produce insulin and a decrease in insulin receptor sensitivity. Epidemiological data show the prevalence of type 2 diabetes mellitus is high in Indonesia and estimated to increase by more than 2.5 times in 2030 compared to 2020. Several factors play an important role in the development and management of diabetes cases, including the management of a good independent diet. People with DM who do not pay attention to their diet can trigger complications and disability. Objective: This study aims to determine the correlation between independent diet management and blood sugar control in patients with type 2 diabetes mellitus. Methods: This study used an observational analytic method with a cross sectional design. The sampling technique was consecutive sampling. Self-management assessment of diet used the Self-Management Diabetic Diet Questionnaire (SMBDQ) which has been adjusted and tested for validity and reliability. Blood sugar measured using a glucometer. Data processing was conducted using Kendall's tau C with a significance level of 95% (α=0.05). Results: There was 79 respondents. There was a correlation with a significance value (P= 0.002) with a low correlation (τ=0.255) between independent diet management and current blood sugar control in patients with type 2 diabetes mellitus. Conclusion: Independent diet management has a significant correlation with blood sugar control in patients type 2 diabetes mellitus. Keywords: Diabetes mellitus, independent diet management, Random Plasma Glucose, Self-Management Diabetic Diet Questionnaire.
Enironmentálny rozmer medzinárodných územných režimov
Authors:   Univerzita Pavla Jozefa Šafárika v Košiciach
Published: December 2023

Zborník príspevkov zo XVII. ročníka študentského sympózia z medzinárodného a európskeho práva konaného dňa 27. októbra 2023.
Lettura in L1 e in L2: una prospettiva multicomponenziale
Authors: Yu Yedi
Published: December 2023

La lettura in L1 e in L2 condividono i processi cognitivi universali, ma differiscono per i processi specifici determinati dalle proprietà delle singole lingue. Al fine di analizzare questi specifici processi linguistici, si adotta il Component Skills Approach, una prospettiva multicomponenziale, che può essere applicata alla lettura in L2 per identificare le diverse abilità richieste dal sistema linguistico d’arrivo. Si crede che confrontare queste ultime con le sotto-abilità di lettura in L1 consenta di prevedere il grado di facilitazione o inibizione nell’elaborazione a livello inferiore nella lettura in L2. Grazie all’osservazione di apprendenti sinofoni di italiano L2, questo studio fornirà un’analisi descrittiva delle sotto-abilità di lettura acquisite nella L1, a confronto con quelle da sviluppare nella L2. Il lavoro prosegue poi discutendo il rapporto fra la lettura in L1 e in L2 e, infine, riassume i risultati di indagini empiriche che si prefiggono di darne una definizione sulla base di teorie differenti.
Hz. Peygamber Devrinde Bir Hadım: Me’bûr el-Kıbtî (ö. 60/679-80) Üzerine Bir Değerlendirme
Authors: Erbay Enes Ensar
Published: December 2023

Kurumsal istihdam anlamında Orta Asur dönemine kadar uzanan hadım varlığı, tarih boyu belirgin bir rağbet gören temel kölelik türleri arasında zikredilebilir. Hadımların Cahiliye devri de dâhil olmak üzere İslâm’ın ilk yıllarındaki varlıklarının eserlerde bahis konusu olduğu gözden kaçmamaktadır. Mukavkıs (ö. 21/642 [?]) tarafından hediye olarak gönderilen köleler içerisinde bulunan Kıbtî kökenli Me’bûr da söz konusu hadımlar arasında yer almaktadır. Hz. Mâriye’nin (ö. 16/637) ağabeyi veya amcaoğlu olduğu yönünde kayıtlar bulunan ve ona hizmet etmesi nedeniyle çeşitli odakların iftira ve suçlamalarına maruz kaldığı anlaşılan Me’bûr hakkında, kaynaklarda oldukça kısıtlı bilgi yer almaktadır. Bu bilgiler çerçevesinde öne çıkan yönünün hadımlığı olduğunu ifade etmekte yarar vardır. Bu makale, Me’bûr’un Mukavkıs tarafından Hz. Peygamber’e gönderilişi, fizyolojik bir özellik olarak hadımlığı ve tematik bir bahis çerçevesinde klasik kaynaklarda yer alan Hz. Mâriye ile ilgili bir suçlamaya konu olması gibi temel tarihî meseleler üzerinde durmaktadır. Bu doğrultuda onun hadımlığı etrafında nakledilen rivayetler, makalenin temel odak noktasını oluşturmaktadır.
El conocimiento especializado del futuro profesor que enseña el espacio proyectivo presupone la capacidad de adaptar tareas matemáticas significativas de acuerdo con las necesidades del contexto. Así, este estudio busca caracterizar el conocimiento especializado del futuro profesor mediante un ciclo de Lesson Study con la adaptación de tareas matemáticas, las cuales son dirigidas principalmente hacía instituciones educativas del sector urbano. Se concluye la necesidad de profundizar en el conocimiento especializado que tienen los docentes en formación desde el componente espacial-métrico. Aunque se trata de una investigación en curso, se esperan obtener como resultado la caracterización del conocimiento especializado que tiene el futuro profesor y las estrategias que usan para adaptar tareas matemáticas según el entorno escolar en el que se van a desarrollar. Esta investigación resulta de gran relevancia, pues permite conocer el conocimiento que tienen los futuros profesores sobre matemáticas al inicio de su carrera.
Authors: Mejía Barajas María Fernanda
Published: December 2023

El conocimiento especializado del futuro profesor que enseña el espacio proyectivo presupone la capacidad de adaptar tareas matemáticas significativas de acuerdo con las necesidades del contexto. Así, este estudio busca caracterizar el conocimiento especializado del futuro profesor mediante un ciclo de Lesson Study con la adaptación de tareas matemáticas, las cuales son dirigidas principalmente hacía instituciones educativas del sector urbano. Se concluye la necesidad de profundizar en el conocimiento especializado que tienen los docentes en formación desde el componente espacial-métrico. Aunque se trata de una investigación en curso, se esperan obtener como resultado la caracterización del conocimiento especializado que tiene el futuro profesor y las estrategias que usan para adaptar tareas matemáticas según el entorno escolar en el que se van a desarrollar. Esta investigación resulta de gran relevancia, pues permite conocer el conocimiento que tienen los futuros profesores sobre matemáticas al inicio de su carrera.
Dialogando com autores sobre o ensino de Física no Ensino Fundamental
Authors: De Moura Pasinatto Joana , Rodrigues de Araujo Rafaele
Published: December 2023

Este estudo foi desenvolvido com o intuito de problematizar a formação dos professores de Ciências para o ensino de Física nos anos finais do Ensino Fundamental a partir de um diálogo com autores que discutem acerca do tema. Nesse sentido, a partir de uma revisão de literatura, apresentamos problematizações sobre a inclusão das disciplinas de Ciências e Física na Educação Básica, um diálogo dos documentos oficiais com o ensino de Física no Ensino Fundamental e discussões sobre a formação dos professores que atuam na disciplina de Ciências. Alguns aspectos que emergiram dessa pesquisa foi a presença marcante dos conceitos físicos nos documentos oficiais para o Ensino Fundamental, a interdisciplinaridade como possibilidade para abordar esses conceitos e também alguns fatores relacionados as dificuldades dos docentes que lecionam na disciplina de Ciências. Nesse estudo ficou evidente que essas dificuldades podem estar relacionadas a falta de domínio dos conteúdos, aos saberes a respeito da metodologia, a linguagem matemática, ao planejamento, a realização de experimentos, entre outros fatores. Dessa forma, se torna válido identificar, principalmente em relação aos professores que não possuem a sua formação inicial em Física, as suas dificuldades para abordar conceitos físicos na disciplina de Ciências.
La Inteligencia artificial y el futuro de la educación superior:
Authors: Zamora Varela Yessika , Mendoza Encinas Maria del Carmen
Published: December 2023

El desarrollo de nuevas tecnologías ha permeado en todos los ámbitos, la educación se considera como una de las menos expuestos a estas. A lo largo de las últimas dos décadas, las instituciones han invertido dinero, esfuerzos y personal en la implementación de las nuevas tecnologías, con el objeto de preparar a los alumnos para su uso y aplicación.

En este contexto, la Inteligencia Artificial cobra gran importancia. En cuanto a los orígenes de este tipo de inteligencia, se puede mencionar que tuvo sus inicios en los años cincuenta, pero es hasta el inicio de este siglo que las compañías desarrolladoras han empezado a ofrecer sus productos al público en general.

El presente trabajo hace una reflexión sobre la Inteligencia Artificial y su papel en la educación superior en América Latina, con una aproximación a los desafíos que enfrenta y las oportunidades que plantea, desde un punto de vista documental. De igual forma, realiza recomendaciones pertinentes para el planteamiento metodológico del uso de la Inteligencia Artificial, en el marco educativo en este nivel.

A través de la aplicación de una encuesta como instrumento de medición del conocimiento, aproximación y opinión sobre la Inteligencia Artificial, se realizó el análisis pertinente para desarrollar de manera adecuada los planteamientos metodológicos. 

El instrumento se constituye de 20 preguntas, algunas son de opción múltiple y otras de respuestas abiertas, que permiten analizar el conocimiento, tipos de uso, percepción de facilidad, utilidad, eficiencia y eficacia de las herramientas o sistemas de inteligencia artificial utilizados.

Los resultados demuestran un uso y aplicación de esta inteligencia, por un porcentaje importante de la población. Al mismo tiempo, presentan la necesidad de desarrollar lineamientos claros para su uso en el ámbito educativo y una clara necesidad de aclarar conceptos éticos relacionados.

Finalmente, en este trabajo se presentan recomendaciones aplicables a la estrategia sobre la Innovación Tecnológica en la Educación, dentro del Consenso de Beijín sobre la Inteligencia Artificial en la Educación. En estas se incluyen los principales aspectos relacionados con el rol del docente, regulaciones sobre con los derechos de autor, requisitos de recursos humanos, tecnológicos y de capacitación e inclusión en la currícula de nivel superior.
Percepción de la calidad de vida en personas recuperadas de covid-19 vs personas no COVID
Authors: Otálora Pineda Julián David , Polanco Barreto Ángela María
Published: December 2023

Introducción: La enfermedad de la COVID-19, se ha convertido en una pandemia al alcanzar más de 5 millones de personas fallecidas en todo el mundo, desde que fue identificado el virus. La enfermedad afecta varios sistemas del organismo, incluyendo el respiratorio, gastrointestinal, cardiovascular y nervioso. Se han reportado casos de secuelas respiratorias, cardiovasculares y neurológicas que duran varias semanas después de superada la enfermedad; sin embargo, no se conocen estudios que evalúen de calidad de vida y genere un proceso comparativo con personas que no lo han tenido. Objetivo: Identificar las diferencias en la percepción de calidad de vida en personas recuperadas de COVID-19 versus la percepción de las personas que no tuvieron la enfermedad. Método: Estudio de enfoque cuantitativo de corte transversal de alcance exploratorio, donde se evaluó la calidad de vida en personas recuperadas de COVID-19, con la escala de calidad de vida WHOQOL BREF, a 30 adultos que se recuperaron de la enfermedad y 30 voluntarios sanos entre los 30 a 50 años de edad. Resultados: en el grupo de personas recuperadas participaron 24 mujeres y 6 hombres, en el grupo de personas sanas participaron 17 mujeres y 13 hombres, se encontró que las personas recuperadas de COVID-19, con una diferencia significativa de 0,05, tuvieron una percepción de calidad de vida mayor al grupo personas que no han presentado la enfermedad.
Construction and application of a multifunctional CHO cell platform utilizing Cre/lox and Dre/rox site-specific recombination systems
Authors: Zhang Chen , Chang Feng , Miao Hui , Fu Yunhui , Tong Xikui , Feng Yu , Zheng Wenyun , Ma Xingyuan
Published: December 2023

During the development of traditional Chinese hamster ovary (CHO) cell lines, target genes randomly integrate into the genome upon entering the nucleus, resulting in unpredictable productivity of cell clones. The characterization and screening of high-yielding cell lines is a time-consuming and expensive process. Site-specific integration is recognized as an effective approach for overcoming random integration and improving production stability. We have designed a multifunctional expression cassette, called CDbox, which can be manipulated by the site-specific recombination systems Cre/lox and Dre/rox. The CDbox expression cassette was inserted at the Hipp11(H11) locus hotspot in the CHO-K1 genome using CRISPR/Cas9 technology, and a compliant CHO-CDbox cell platform was screened and obtained. The CHO-CDbox cell platform was transformed into a pool of EGFP-expressing cells using Cre/lox recombinase-mediated cassette exchange (RMCE) in only 2 weeks, and this expression remained stable for at least 75 generations without the need for drug stress. Subsequently, we used the Dre/rox system to directly eliminate the EGFP gene. In addition, two practical applications of the CHO-CDbox cell platform were presented. The first was the quick construction of the Pembrolizumab antibody stable expression strain, while the second was a protocol for the integration of surface-displayed and secreted antibodies on CHO cells. The previous research on site-specific integration of CHO cells has always focused on the single functionality of insertion of target genes. This newly developed CHO cell platform is expected to offer expanded applicability for protein production and gene function studies.
Quantitative analysis of preferential utilization of AAV ITR as the packaging terminal signal
Authors: Li Xin , Miller Lohra Mickelle , Chrzanowski Matthew , Tian Jiahe , Jarrold Martin F. , Herzog Roland W. , Xiao Weidong , Draper Benjamin , Zhang Junping
Published: December 2023

Genetic engineering advances have led to recombinant adeno-associated virus (rAAV) becoming an invaluable tool for the development of effective gene therapies. The production of rAAV is susceptible to off-target heterogeneous packaging, the effects of which are still being understood. Here, rAAV vectors with four-genome lengths were produced using both adherent and suspension HEK293 cells to understand the 5′ITR termination. AAV8 vectors were produced from the human FVIII plasmid for a full-length cargo of 4,707 nucleotides with specific truncations, creating smaller genomes. Conventionally, rAAV is characterized by differentiating empty capsids from full capsids, but for this work, that description is incomplete. The small genomes in this study were characterized by charge detection-mass spectrometry (CD-MS). Using CD-MS, packaged genomes in the range conventionally attributed to partials were resolved and quantified. In addition, alkaline gels and qPCR were used to assess the identity of the packaged genomes. Together, these results showed a propensity for unit-length genomes to be encapsidated. Packaged genomes occurred as replication intermediates emanating from the 5′ITR, indicating that HEK293 cells prefer unit-length genomes as opposed to the 5′ITR termination and heterogeneous DNA packaging observed previously from Sf9 cell systems. As both manufacturing processes are used and continually assessed to produce clinical material, such an understanding will benefit rAAV design for basic research and gene therapy.
In-hospital and 1-year outcomes of patients without modifiable risk factors presenting with acute coronary syndrome undergoing PCI: a Sex-stratified analysis
Authors: Sheikhy Ali , Fallahzadeh Aida , Jameie Mana , Aein Afsaneh , Masoudkabir Farzad , Maghsoudi Milad , Tajdini Masih , Salarifar Mojtaba , Jenab Yaser , Pourhosseini Hamidreza , Mehrani Mehdi , Alidoosti Mohammad , Vasheghani-Farahani Ali , Hosseini Kaveh
Published: December 2023

Aim

A considerable proportion of patients admitted with acute coronary syndrome (ACS) have no standard modifiable cardiovascular risk factors (SMuRFs: hypertension, diabetes mellitus, dyslipidemia, and cigarette smoking). The outcomes of this population following percutaneous coronary intervention (PCI) are debated. Further, sex differences within this population have yet to be established.

Methods

This retrospective cohort study included 7,847 patients with ACS who underwent PCI. The study outcomes were in-hospital mortality, all-cause mortality, and major adverse cardio-cerebrovascular events (MACCE). The association between the absence of SMuRFs (SMuRF-less status) and outcomes among all the patients and each sex was assessed using logistic and Cox proportional hazard regressions.

Results

Approximately 11% of the study population had none of the SMuRFs. During 12.13 [11.99–12.36] months of follow-up, in-hospital mortality (adjusted-odds ratio (OR):1.51, 95%confidence interval (CI): 0.91–2.65, P:0.108), all-cause mortality [adjusted-hazard ratio (HR): 1.01, 95%CI: 0.88–1.46, P: 0.731], and MACCE (adjusted-HR: 0.93, 95%CI:0.81–1.12, P: 0.412) did not differ between patients with and without SMuRFs. Sex-stratified analyses recapitulated similar outcomes between SMuRF+ and SMuRF-less men. In contrast, SMuRF-less women had significantly higher in-hospital (adjusted-OR: 3.28, 95%CI: 1.92–6.21, P < 0.001) and all-cause mortality (adjusted-HR:1.41, 95%CI: 1.02–3.21, P: 0.008) than SMuRF+ women.

Conclusions

Almost one in 10 patients with ACS who underwent PCI had no SMuRFs. The absence of SMuRFs did not confer any benefit in terms of in-hospital mortality, one-year mortality, and MACCE. Even worse, SMuRF-less women paradoxically had an excessive risk of in-hospital and one-year mortality.
Assessment of causal association between differentiated thyroid cancer and disordered serum lipid profile: a Mendelian randomization study
Authors: Ma Qiang , Li Yu , An Lijuan , Guo Liang , Liu Xiaokang
Published: December 2023

Background

Research has shown that the disordered serum lipid profile may be associated with the risk of differentiated thyroid cancer (DTC). Whether this association reflect causal effect is still unclear. The aim of this study was to evaluate the causality of circulating lipoprotein lipids on DTC.

Methods

Mendelian randomization (MR) analysis was conducted to evaluate the relationship between the circulating lipoprotein lipids and DTC risk using single-nucleotide polymorphisms (SNPs) from a genome-wide association (GWA) study containing a high-incidence Italian population of 690 cases samples with DTC and 497 controls.

Results

Univariate and multivariate mendelian randomization analysis demonstrated that ‘total cholesterol’, ‘HDL cholesterol’, ‘apolipoprotein B’ and ‘ratio of apolipoprotein B to apolipoprotein A1’ were correlated with DTC. According to sensitivity analysis, our results were reliable. Furthermore, multivariate analysis revealed that there is no causative association between DTC and any of the many cause factors when they interact with one another, suggesting that there was a deep interaction between the four factors, which could affect each other. Finally, the mechanism of the related effects each other as well as the target genes with significant SNP regulatory effects in DTC was explored by conducting functional enrichment analysis and constructing the regulatory networks.

Conclusions

We obtained four exposure factors (total cholesterol, HDL cholesterol, apolipoprotein B and ratio of apolipoprotein B to apolipoprotein A1) closely related to DTC, which laid a theoretical foundation for the treatment of DTC.
Clinical and imaging associations for non-ketotic hyperglycemic chorea: a case-control study
Authors: Liang Zhuo-Yuan , Lu Zhi-Hao , Qu Jian-Feng , Chen Yang-Kun
Published: December 2023

Background

The non-ketotic hyperglycemic chorea (NKHC) was a rare complication for patients with diabetes mellitus, but not been well studied. In the present research, we aimed to investigate the clinical and imaging characteristics of NKHC and explore the potential association.

Methods

We performed a case-control study with patients diagnosed as NKHC. The patients with group of NKHC were retrospectively recruited, while the matched group were set to screened patients with diabetes mellitus but no NKHC at a 1:3 ratio. The clinical and imaging data were collected for all the participants of the two groups. Firstly, Correlation analysis was conducted to test the difference of all the variables between the NKHC group and matched group. Then, the putative associated factors for NKHC were further identified.

Results

Eleven men and 9 women with NKHC and 60 matched participants were analyzed. The mean age of the NKHC group was 68.5 ± 14.9 years. Participants with NKHC were more likely to have a higher glycosylated hemoglobin (HbA1c) level (13 ± 2.82 vs. 10.57 ± 2.71, P<0.001), and a higher frequency of renal dysfunction (estimated glomerular filtration rates <60 ml/min/1.73m2) (55% vs. 20%, P=0.005). Logistic regression analyses showed that both higher HbA1c and renal dysfunction were significantly correlated with NKHC.

Conclusion

A higher value of HbA1c and renal dysfunction may be associated with the occurrence of NKHC.
The effects of anthropogenic noise and urban habitats on song structure in a vocal mimic; the gray catbird (Dumetella carolinensis) sings higher frequencies in noisier habitats
Authors: Rhodes Morgan L. , Ryder T. Brandt , Evans Brian S. , To Jennifer C. , Neslund Elizabeth , Will Christopher , O’Brien Lauren E. , Moseley Dana L.
Published: December 2023

In urban and human-modified landscapes, animals face novel selection pressures resulting from differences in habitat structure and increased anthropogenic noise. Urban noise pollution can negatively impact songbirds because low-frequency noise often masks portions of birds’ mating signals and reduces signal transmission. Previous research has demonstrated that the songs of birds in more urban habitats have structural differences that can enhance signal transmission when noise is present. The majority of these studies have focused on species that deliver short, stereotyped songs and have limited repertoires. Gray catbirds (Dumetella carolinensis, family: Mimidae) sing long bouts containing imitated, improvised, and invented song elements, and therefore may have an increased ability to vary songs in response to noise. We hypothesized that aspects of developed habitats including loud anthropogenic noise and changes to land cover would impact catbirds’ song structural parameters, including song minimum, peak, and maximum frequency, frequency bandwidth, and entropy. We recorded and processed songs from 42 male catbirds and analyzed over 18,000 song elements from sites along an urban gradient from western Virginia to the Washington, DC metropolitan region. We quantified the urban intensity at each site-centroid based on percent canopy cover, percent impervious surface, and noise level. Song features such as minimum, maximum, and peak frequency increased significantly as noise levels increased, demonstrating that catbirds in noisier areas sing higher frequency songs compared to individuals in quieter habitats. Land cover variables also significantly predicted certain song features such as maximum frequency (impervious surface) or entropy (canopy cover). These structural differences in catbird song can limit the negative effects of environmental noise-masking, even for their long song bouts, and suggest that vocal mimics respond to anthropogenic noise. Future studies could investigate repertoire size and composition along an urban gradient and if these structural differences lead to functional consequences for the songs of vocal mimics.
Importance of comprehensive genetic testing for patients with suspected vascular Ehlers–Danlos syndrome: a family case report and literature review
Authors: Wei Xianda , Zhou Xu , Xie BoBo , Shi Meizhen , Gui Chunrong , Liu Bo , Li Caiyan , Zhang Chi , Luo Jiefeng , Mi Cundong , Gui Baoheng
Published: December 2023

Vascular Ehlers–Danlos syndrome (vEDS), the most severe type of Ehlers–Danlos syndrome, is caused by an autosomal-dominant defect in the COL3A1 gene. In this report, we describe the clinical history, specific phenotype, and genetic diagnosis of a man who died of vEDS. The precise diagnosis of this case using whole-exome sequencing provided solid evidence for the cause of death, demonstrating the practical value of genetic counseling and analysis. Early diagnosis for the proband’s son, who was also affected by vEDS, revealed initial complications of vEDS in early childhood, which have rarely been reported. We also reviewed the literature on COL3A1 missense mutations and related phenotypes. We identified an association between digestion tract events and non-glycine missense variants, which disproves a previous hypothesis regarding the genotype–phenotype correlation of vEDS. Our results demonstrate the necessity of offering comprehensive genetic testing for every patient suspected of having vEDS.
The PRotective Effect of Maternal Immunisation on preTerm birth: characterising the Underlying mechanisms and Role in newborn immune function: the PREMITUR study protocol
Authors: Giles Michelle L. , Cole Stephen , O’Bryan Jessica , Krishnaswamy Sushena , Ben-Othman Rym , Amenyogbe Nelly , Davey Mary-Ann , Kollmann Tobias
Published: December 2023

Maternal immunisation, a low cost and high efficacy intervention is recommended for its pathogen specific protection. Evidence suggests that maternal immunisation has another significant impact: reduction of preterm birth (PTB), the single greatest cause of childhood morbidity and mortality globally. Our overarching question is: how does maternal immunisation modify the immune system in pregnant women and/or their newborn to reduce adverse pregnancy outcomes and enhance the newborn infant’s capacity to protect itself from infectious diseases during early childhood? To answer this question we are conducting a multi-site, prospective observational cohort study collecting maternal and infant biological samples at defined time points during pregnancy and post-partum from nulliparous women. We aim to enrol 400 women and determine the immune trajectory in pregnancy and the impact of maternal immunisation (including influenza, pertussis and/or COVID-19 vaccines) on this trajectory. The results are expected to identify areas that can be targeted for future intervention studies.
Cystoseira myrica: from beach-cast seaweed to fucoidan with antioxidant and anticoagulant capacity
Authors: Dhahri Manel
Published: December 2023

This study highlights the potential of the brown algae Cystoseira myrica, collected from raw beach seaweed wastes, as a reliable source of bioactive fucoidan. Fucoidans are natural bioactive sulfated polysaccharides that are gaining popularity worldwide due to their diverse biochemical composition, attractive physical properties, and functional pharmacological activities. The aim of this work is to valorize the beach-Accumulated brown algae C. myrica by fucoidan extraction and to investigate its anticoagulant and antioxidant activity. Fucoidan was extracted using several steps of papain digestion followed by cetyltrimethylammonium bromide (CTAB) precipitation and calcium chloride treatment to avoid the coextraction of other polysaccharides. Structural features of the extracted fucoidan were investigated by Fourier transform infrared spectroscopy (FT-IR), solid-state nuclear magnetic resonance (NMR) and high-resolution mass spectroscopy. Agarose gel electrophoresis was used to confirm the purity of the isolated fucoidan from C. myrica. Anticoagulant properties were studied in vitro by activated partial thromboplastin time (aPTT) and prothrombin time (PT) assays. Furthermore, the antioxidant activity was investigated by 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and Fe chelating ability. Our results indicate that the approach used was effective in extracting fucoidan with a yield of 3.07%, a high amount of sulfate (27.79%), and fucose was found to be the major monosaccharide component. The extracted fucoidan showed an interesting anticoagulant activity. It prolonged aPTT significantly at a concentration of 10 µg/ml and prolonged PT at high doses. This demonstrated that fucoidan from C. myrica may affect intrinsic pathways while having little impact on the extrinsic mechanism of coagulation. However, the extracted fucoidan significantly exhibited an interesting antioxidant capacity as shown by the higher ABTS radical scavenging activity and Ferrous ion-chelating effect. The current findings suggest that fucoidan isolated from C. myrica has unique structural, antioxidant and anticoagulant properties and offers innovative therapeutic possibilities.
Automated deep bottleneck residual 82-layered architecture with Bayesian optimization for the classification of brain and common maternal fetal ultrasound planes
Authors: Rauf Fatima , Khan Muhammad Attique , Bashir Ali Kashif , Jabeen Kiran , Hamza Ameer , Alzahrani Ahmed Ibrahim , Alalwan Nasser , Masood Anum
Published: December 2023

Despite a worldwide decline in maternal mortality over the past two decades, a significant gap persists between low- and high-income countries, with 94% of maternal mortality concentrated in low and middle-income nations. Ultrasound serves as a prevalent diagnostic tool in prenatal care for monitoring fetal growth and development. Nevertheless, acquiring standard fetal ultrasound planes with accurate anatomical structures proves challenging and time-intensive, even for skilled sonographers. Therefore, for determining common maternal fetuses from ultrasound images, an automated computer-aided diagnostic (CAD) system is required. A new residual bottleneck mechanism-based deep learning architecture has been proposed that includes 82 layers deep. The proposed architecture has added three residual blocks, each including two highway paths and one skip connection. In addition, a convolutional layer has been added of size 3 × 3 before each residual block. In the training process, several hyper parameters have been initialized using Bayesian optimization (BO) rather than manual initialization. Deep features are extracted from the average pooling layer and performed the classification. In the classification process, an increase occurred in the computational time; therefore, we proposed an improved search-based moth flame optimization algorithm for optimal feature selection. The data is then classified using neural network classifiers based on the selected features. The experimental phase involved the analysis of ultrasound images, specifically focusing on fetal brain and common maternal fetal images. The proposed method achieved 78.5% and 79.4% accuracy for brain fetal planes and common maternal fetal planes. Comparison with several pre-trained neural nets and state-of-the-art (SOTA) optimization algorithms shows improved accuracy.