bluuebunny/crossref_metadata_2025 huggingface dataset:
A Collocation Numerical Method for Highly Oscillatory Algebraic Singular Volterra Integral Equations
Authors: SAIRA  , Ma Wen-Xiu , Liu Guidong
Published: January 2024

The highly oscillatory algebraic singular Volterra integral equations cannot be solved directly. A collocation numerical method is proposed to overcome the difficulty created by the highly oscillatory algebraic singular kernel. This paper is composed primarily of two methods—the piecewise constant collocation method and the piecewise linear collocation method—in which uniformly distributed nodes serve as collocation points. For the efficient computation of highly oscillatory and algebraic singular integrals, the steepest descent method as well as the Gauss–Laguerre and generalized Gauss–Laguerre quadrature rules are employed. Consequently, the resulting linear system is solved for the unknown function approximated by the Lagrange interpolation polynomial. Detailed theoretical analysis is carried out and numerical experiments showing high accuracy are also presented to confirm our analysis.
Influences of Different Acid Solutions on Pore Structures and Fractal Features of Coal
Authors: Zhang Jingshuo , Ni Xiaoming , Liu Xiaolei , Su Erlei
Published: January 2024

The effect of different acids on the pore structure and fractal characteristics of micropores and mesopores was determined with the help of low-temperature liquid nitrogen adsorption, X-ray diffraction, and the Frenkel–Halsey–Hill (FHH) model by using Yuwu coal as a sample and placing it in acidic environments, such as HF, HCl, HNO3, and CH3COOH. The results show that the acidization effects of HF and CH3COOH are separately dominated by the micropore and mesopore formation effects, while HCl and HNO3 mainly play their roles in expanding mesopores. After acidization, the surface fractal dimensions D1 and D1′ of micropores and mesopores in coal are always negatively correlated with the total specific surface area SBET, specific surface area Smic of micropores, and specific surface area Smes of mesopores. After being acidized by HF, D2 is negatively correlated with the total volume Vtot and the corresponding micropore volume Vmic, while acidization with HCl and HNO3 leads to the opposite result. After being acidized by CH3COOH, D2 has a negative correlation with Vtot and a positive correlation with Vmic. The structural fractal dimensions D2′ of mesopores in samples acidized by HF and CH3COOH are positively correlated with both the volume Vtot and mesopore volume Vmes, while it is the opposite for samples acidized by HNO3. D2′ of coal samples acidized by HCl is negatively correlated with Vtot while positively correlated with Vmes.
Bang-Bang Property and Time-Optimal Control for Caputo Fractional Differential Systems
Authors: Abel-Gaid Shimaa H. , Qamlo Ahlam Hasan , Mohamed Bahaa Gaber
Published: January 2024

In this paper, by using the controllability method, a bang-bang property and a time optimal control problem for time fractional differential systems (FDS) are considered. First, we formulate our problem and prove the existence theorem. We then state and prove the bang-bang theorem. Finally, we state the optimality conditions that characterize the optimal control. Some application examples are given to illustrate our results.
A Discrete-Time Fractional-Order Flocking Control Algorithm of Multi-Agent Systems
Authors: Chen Haotian , He Ming , Han Wei , Liu Sicong , Wei Chenyue
Published: January 2024

In this paper, a discrete-time fractional flocking control algorithm of multi-agent systems is put forward to address the slow convergence issue of multi-agent systems. Firstly, by introducing Grünwald-Letnikov (G-L) fractional derivatives, the algorithm allows agents to utilize historical information when updating their states. Secondly, based on the Lyapunov stability theory, the convergence of the algorithm is proven. Finally, simulations are conducted to verify the effectiveness of the proposed algorithm. Comparisons are made between the proposed algorithm and other methods. The results show that the proposed algorithm can effectively improve the convergence speed of multi-agent systems.
Fractal Analysis on the Crushing Characteristics of Soil-Soft Rock Mixtures under Compaction
Authors: Hu Fengyun , Zhang Keneng , Zhu Kaofei , Li Bintian , Zhang Zhao , He Yong
Published: January 2024

Soil-rock mixtures (SRM) are extensively utilized as filling materials in engineering slopes and roadbeds. A comprehensive understanding of the crushing characteristics of SRM during compaction is essential for precisely controlling its mechanical properties, particularly when dealing with SRM comprising soft rock blocks. This study conducted heavy compaction and screening tests to investigate the crushing and compaction behaviors of soil-soft rock mixture (SSRM) with varying coarse particle content (P5 content), the primary focus was primarily on analyzing the double fractal characteristics of coarse and fine particles. The research findings are as follows: with the increase of P5 content, the maximum dry density of SSRM initially rises and then declines, reaching its peak when P5 content is 70%. Soft rock blocks in SSRM exhibit extreme fragility during compaction, the crushing index of coarse particles exhibits a linear increase with the rise in P5 content, whereas the crushing index of fine particles displays a “double peak” characteristic. After compaction, a linear positive correlation is observed between the fractal dimension and the crushing index of coarse and fine particles. With the increase in P5 content, the slope of the relationship curve between the fractal dimension and the crushing index of coarse particles remains relatively constant, while the intercept gradually decreases. Moreover, the fractal dimension of fine particles effectively reflects the compaction characteristics of SSRM, and the relationship between the fractal dimension of fine particles and dry density aligns with the compaction curve of SSRM.
Application of Fiber Optics for Completion Design Optimization: A Methodological Approach and Key Findings
Authors: Fathi Ebrahim , Belyadi Fatemeh , Adenan Mohammad Faiq , Pacheco Christian
Published: January 2024

This study investigates the application of fiber optic technology to optimize completion design in a hydraulic fracture stimulation for Marcellus Shale Reservoir. With a focus on improving cluster efficiencies and overcoming interstage communication challenges, the research utilizes real-time data from distributed acoustic (DAS), temperature (DTS), and strain (DSS) measurements. The methodology comprises a comprehensive analysis of completion and stimulation reports, fiber optics, microseismic data, and well logs. Conducted at the MSEEL well pads, MIP, and Boggess, and equipped with permanent and deployable fiber optic cables, this study emphasizes that engineered/geomechanical completion design leads to sustained cluster efficiency and stage production performance. Inefficient cluster efficiencies are primarily linked to fracture communication. Recommendations include employing a geomechanical completion design, avoiding non-uniform high natural fracture zones during hydraulic fracture stimulations, implementing short stage length, and using more 100 mesh sand. These insights, derived from correlations between fracture counts, distributed strain sensing (DSS), cluster efficiency, production logging, and production data, offer significant implications for optimizing completion design in unconventional reservoirs. The effective application of fiber optic technology, providing real-time DAS, DTS, and slow strain data, proves instrumental in addressing interstage communication challenges, contributing to improved reservoir performances and cost-effective operations in hydraulic fracture stimulations.
Understanding Hypertriglyceridemia: Integrating Genetic Insights
Authors: Alves Mara , Laranjeira Francisco , Correia-da-Silva Georgina
Published: January 2024

Hypertriglyceridemia is an exceptionally complex metabolic disorder characterized by elevated plasma triglycerides associated with an increased risk of acute pancreatitis and cardiovascular diseases such as coronary artery disease. Its phenotype expression is widely heterogeneous and heavily influenced by conditions as obesity, alcohol consumption, or metabolic syndromes. Looking into the genetic underpinnings of hypertriglyceridemia, this review focuses on the genetic variants in LPL, APOA5, APOC2, GPIHBP1 and LMF1 triglyceride-regulating genes reportedly associated with abnormal genetic transcription and the translation of proteins participating in triglyceride-rich lipoprotein metabolism. Hypertriglyceridemia resulting from such genetic abnormalities can be categorized as monogenic or polygenic. Monogenic hypertriglyceridemia, also known as familial chylomicronemia syndrome, is caused by homozygous or compound heterozygous pathogenic variants in the five canonical genes. Polygenic hypertriglyceridemia, also known as multifactorial chylomicronemia syndrome in extreme cases of hypertriglyceridemia, is caused by heterozygous pathogenic genetic variants with variable penetrance affecting the canonical genes, and a set of common non-pathogenic genetic variants (polymorphisms, using the former nomenclature) with well-established association with elevated triglyceride levels. We further address recent progress in triglyceride-lowering treatments. Understanding the genetic basis of hypertriglyceridemia opens new translational opportunities in the scope of genetic screening and the development of novel therapies.
Exploring the Influence of Climate Change on Earthen Embankments with Expansive Soil
Authors: Ghosh Debayan , Banerjee Aritra , Puppala Anand J. , Kumar Prince
Published: January 2024

Climate change is known to cause alterations in weather patterns and disturb the natural equilibrium. Changes in climatic conditions lead to increased environmental stress on embankments, which can result in slope failures. Due to wetting–drying cycles, expansive clayey soil often swells and shrinks, and matric suction is a major factor that controls the behavior. Increased temperature accelerates soil evaporation and drying, which can cause desiccation cracks, while precipitation can rapidly reduce soil shear strength. Desiccated slopes on embankments built with such soils can cause surficial slope failures after intense precipitation. This study used slope stability analysis to quantify how climate-change-induced extreme weather affects embankments. Historic extreme climatic events were used as a baseline to estimate future extremes. CMIP6 provided historical and future climatic data for the study area. An embankment was numerically modeled to evaluate the effect on slope stability due to the precipitation change induced by climate change. Coupled hydro-mechanical finite element analyses used a two-dimensional transient unsaturated seepage model and a limit equilibrium slope stability model. The study found that extreme climatic interactions like precipitation and temperature due to climate change may reduce embankment slope safety. The reduction in the stability of the embankment due to increased precipitation resulting from different greenhouse gas emission scenarios was investigated. The use of unsaturated soil strength and variation of permeability with suction, along with the phase transition of these earthen embankments from near-dry to near-saturated, shows how unsaturated soil mechanics and the hydro-mechanical model can identify climate change issues on critical geotechnical infrastructure.
The Third Dimension of Eye Care: A Comprehensive Review of 3D Printing in Ophthalmology
Authors: Lin Neil , Gagnon Maryse , Wu Kevin Y.
Published: January 2024

Three-dimensional (3D) printing is a process in which materials are added together in a layer-by-layer manner to construct customized products. Many different techniques of 3D printing exist, which vary in materials used, cost, advantages, and drawbacks. Medicine is increasingly benefiting from this transformative technology, and the field of ophthalmology is no exception. The possible 3D printing applications in eyecare are vast and have been explored in the literature, such as 3D-printed ocular prosthetics, orbital implants, educational and anatomical models, as well as surgical planning and training. Novel drug-delivery platforms have also emerged because of 3D printing, offering improved treatment modalities for several ocular pathologies. Innovative research in 3D bioprinting of viable tissues, including the cornea, retina, and conjunctiva, is presenting an avenue for regenerative ophthalmic therapies in the future. Although further development in printing capabilities and suitable materials is required, 3D printing represents a powerful tool for enhancing eye health.
Role of Social Support in Screening Colonoscopy/Sigmoidoscopy Uptake among U.S. Adults
Authors: Ansa Benjamin E. , Datta Biplab , Ibrahim Samah , Islam KM Monirul , Saucier Ashley , Coffin Janis
Published: January 2024

Colorectal cancer (CRC) is a major clinical and public health burden. Screening has been shown to be effective in preventing CRC. In 2021, less than 72% of adult Americans had received CRC screening based on the most recent guidelines. This study examined the relationship between social support and screening colonoscopy or sigmoidoscopy uptake among U.S. adults and the socioeconomic factors that impact the relationship. We conducted a cross-sectional study using the 2021 National Health Interview Survey (NHIS) data for 20,008 U.S. adults to assess the weighted rates of screening colonoscopy or sigmoidoscopy among individuals with strong, some, and weak social support. Adjusted binary logistic regression models were utilized to obtain the weighted odds of receiving a screening colonoscopy or sigmoidoscopy among adults with different levels of social support and socioeconomic status. About 58.0% of adults who reported having colonoscopy or sigmoidoscopy had strong social support, compared to 52.0% who had some or weak social support. In addition, compared to adults with weak social support, the weighted adjusted odds of having colonoscopy or sigmoidoscopy were 1.0 (95% C.I. = 0.994, 0.997; p < 0.001) and 1.3 (95% C.I. = 1.260, 1.263; p < 0.001) for adults with some and strong social support, respectively. Socioeconomic differences were observed in the odds of colonoscopy or sigmoidoscopy uptake based on having strong social support. Having strong social support is an important factor in increasing colonoscopy or sigmoidoscopy screening uptake. Policies and interventions that enhance social support among adults for screening colonoscopy or sigmoidoscopy are warranted.
A Qualitative, Small-Sample Study of Employment Challenges for People with Disabilities in Saudi Arabia
Authors: Abed Mohaned G. , Abed Lowai G. , Shackelford Todd K.
Published: January 2024

Many people live with disabilities and many countries worldwide are acting to provide people with disabilities opportunities to find and sustain gainful employment. Notwithstanding, people with disabilities still do not have the same access to employment as their counterparts without disabilities. Although some research has investigated these issues in Western countries, very little research has investigated these issues in the Middle East, in general, and in Saudi Arabia, in particular. The aim of the present study is to gain an initial understanding of the employment experiences of people with disabilities and prospective employers of people with disabilities in Saudi Arabia. We identify several potential obstacles preventing people with disabilities from securing and maintaining gainful employment in Saudi Arabia. We secured qualitative data from semi-structured interviews with five prospective employers and five individuals with disabilities in Saudi Arabia. We focused on identifying barriers to employment. The impact of demographic factors such as age, gender, and education on employment opportunities was also examined. The results suggest several practical conclusions and recommendations relating to improving and enhancing opportunities for employment for people with disabilities in Saudi Arabia.
Effectiveness and Health Outcomes of Collaborative Nurse Prescribing for Patients Anticoagulated with Antivitamin K in Primary Care: A Study Protocol
Authors: Lara Juan Carlos Palomo , March-Cerdà Joan Carles , Ponce-Blandón José Antonio , Pabón-Carrasco Manuel , Jiménez-Picón Nerea
Published: January 2024

The development of collaborative nurse prescribing (NP) in Andalusia (Spain) in 2018 gives us the opportunity to measure the impact of this practice. Scientific evidence indicates that prescribing is not more costly when performed by nurses and, in fact, is more economical in some cases. The aim of this study is to determine the effects of NP on the effectiveness, health outcomes and adverse events related to prescribing including in the follow-up of patients treated with antivitamin K oral anticoagulants in primary care (PC) by nurses. The design is a randomized clinical trial. The population comprises 1208 anticoagulated patients in 2019. The sample size calculation considers an alpha error of 0.05, a power of 99% and an effect size of 0.5, resulting in 127 users per group. Therefore, a total sample of 254 participants is needed. However, as the project intends to treat patients it will include the universal sample that meets the criteria in the two health centers participating in the study, with 575 participants in total. Data collection was carried out in the PC District of the Alamillo-San Jerónimo Clinical Management Unit of Sevilla for one year from January 2020. Data analysis is performed using the SPSS Statistics 25 package. We intend to study if nurse collaborative prescription in the follow-up and management of patients taking antivitamin K oral anticoagulants in PC is as effective as the traditional approach to follow-ups carried out by a family physician.
The Impact of the Physical Activity Level on Sarcopenic Obesity in Community-Dwelling Older Adults
Authors: Choi Seongmin , Chon Jinmann , Yoo Myung Chul , Shim Ga Yang , Kim Minjung , Kim Miji , Soh Yunsoo , Won Chang Won
Published: January 2024

Previous studies have reported that low levels of physical activity result in sarcopenic obesity (SO). However, the effects of specific intensities of physical activity on SO and the optimal amount of physical activity for lowering the prevalence of SO have not been well studied. This study aimed to identify the effects of physical activity levels and intensity on SO and the optimal amount of physical activity related to a lower prevalence of SO. This cross-sectional study used data from the nationwide Korean Frailty and Aging Cohort Study (KFACS), which included 2071 older adults (1030 men, 1041 women). SO was defined according to the criteria of the European Society for Clinical Nutrition Metabolism (ESPEN) and the European Association for the Study of Obesity (EASO). Multivariate logistic regression analysis was performed to investigate the association between the physical activity level and SO. The high activity group had a significantly lower prevalence of SO than the non-high activity (low and moderate activity) group. On the other hand, moderate-intensity physical activity was associated with a lower prevalence of SO. A total physical activity energy expenditure of > 3032 kcal/week (433 kcal/day) for men and 2730 kcal/week (390 kcal/day) for women was associated with a reduced prevalence of SO. The high physical activity and total physical energy expenditure described above may be beneficial for reducing the prevalence of SO.
Inverse Association between Exercising Blood Pressure Response and Left Ventricular Chamber Size and Mass in Women Who Habitually Resistance Train
Authors: Matthews Evan L. , Guers John J. , Ramick Meghan G. , Hosick Peter A.
Published: January 2024

Exercise is a major modifiable lifestyle factor that leads to temporarily increased systolic blood pressure (SBP), which is thought to influence left ventricular mass normalized to body surface area (LVM/BSA). This relationship has never been studied in women who habitually perform resistance exercise. Purpose: To determine if a direct correlation exists between the SBP response to resistance exercise (change from rest; eSBP) and LVM/BSA in young healthy women who habitually resistance train. Methods: Leg extension resistance exercise was performed while continuously monitoring blood pressure using finger plethysmography. LVM was estimated using echocardiography. Data are shown as mean ± SD. Results: Thirty-one women participated (age 23 ± 3 years, height 164 ± 7 cm, body mass 63.7 ± 10.3 kg). Resting SBP (110 ± 8 mmHg, r = 0.355, p = 0.049) was shown to be directly correlated to LVM/BSA (72.0 ± 28.4 g/m2). Conversely, eSBP (30.8 ± 14.6 ∆mmHg, r = −0.437, p = 0.014) was inversely related to LVM/BSA. eSBP was not correlated to interventricular septum width (0.88 ± 0.12 cm, r = −0.137, p = 0.463) or posterior wall thickness (0.91 ± 0.15 cm, r = −0.084, p = 0.654). eSBP was inversely related to left ventricle internal diameter during diastole (LVIDd) (4.25 ± 0.33 cm, r = −0.411, p = 0.021). Conclusion: Counter to the hypothesis, these data suggest an inverse association between eSBP during resistance exercise and LVM/BSA in healthy young women who resistance train. This relationship is due to a smaller LVIDd with greater eSBP.
Disinformation by Proponents of Perkins’ Patent “Metallick Tractors” (1798–1806) to Sway Public Opinion in Britain in Favor of a Fraudulent Therapy
Authors: Lanska Douglas J.
Published: January 2024

In 1796, American physician Elisha Perkins patented “metallick Tractors” for the treatment of various ailments, particularly those associated with pain. They were subsequently rapidly and widely disseminated in the United States and Great Britain based on testimonials and deceptive marketing tactics. Dissemination was facilitated by endorsements from prominent physicians, politicians, and clergymen; quasi-theoretical, handwaving explanations of efficacy based on Galvani’s then-current experiments; and the procedure’s apparent safety and simplicity. However, blinded placebo-controlled trials in Great Britain using sham devices demonstrated that the therapy was ineffective. In response, in the period from 1798 to 1806, Perkinists unleashed a barrage of disinformation (ad hominem attacks, misleading arguments, unethical propaganda tactics, and poetic and graphic satire) to sway public opinion in favor of the fraudulent therapy and against its critics. The disinformation slowed the abandonment of “tractoration”, but higher-level scientific argumentation ultimately prevailed. The Perkinist disinformation campaign had antecedents with the Mesmerist disinformation campaign in the mid-1780s. Similar propaganda tactics are still widely employed to encourage the purchase and use of disproven or fraudulent therapies, as evidenced by propaganda from adherents of acupuncture in response to negative clinical trials and from supporters of unsafe and ineffective therapies promulgated during COVID-19.
Seaweed Polysaccharides as Potential Biostimulants in Turnip Greens Production
Authors: Mamede Mariana , Cotas João , Pereira Leonel , Bahcevandziev Kiril
Published: January 2024

Seaweed polysaccharides can act as substitutes for synthetic compounds present in commercial stimulants and fertilizers used in agriculture to improve crop yields and vigor. In this study, three different polysaccharides (alginate, agar, and carrageenan) were extracted from one brown seaweed, Saccorhiza polyschides, and two red seaweeds, Gracilaria gracilis and Chondrus crispus, respectively, and applied to potted turnip greens (Brassica napus L.), with the intention to analyze their impact on plant growth, development, and metabolism. Turnip greens treated with polysaccharides, especially carrageenan of C. crispus, showed the best results in improving the crop productivity in terms of plant length and weight, number of leaves, nutrient and pigment content, and soil fertility compared with turnip greens from the negative control or those treated with a commercial leaf fertilizer. λ-carrageenan extracted from the tetrasporophyte generation of C. crispus had the highest bioactivity and positive effect on turnip greens among all treatments. λ-carrageenan has been shown to improve plant growth; increase the plant’s biomass (plant leaves: CC(T) (40.80 ± 5.11 g) compared to the positive control (15.91 ± 15.15 g)) and root system; enhance photosynthetic activity; increase the uptake of soil nutrients; and protect plants against abiotic and biotic stresses, stimulating the production of secondary metabolites and managing its defense pathways. Seaweed-extracted polysaccharides have the potential to be used in sustainable agriculture.
Poverty–Food Insecurity Nexus in the Post-Construction Context of a Large Hydropower Dam in the Brazilian Amazon
Authors: Johansen Igor Cavallini , Calvi Miquéias Freitas , Luz Verônica Gronau , Segall-Corrêa Ana Maria , Arantes Caroline C. , Isaac Victoria Judith , Utsunomiya Renata , Reis Vanessa Cristine e Souza , Moran Emilio F.
Published: January 2024

Within the 2030 Sustainable Development Agenda, large hydropower dams are positioned as a sustainable energy source, notwithstanding their adverse impacts on societies and ecosystems. This study contributed to ongoing discussions about the persistence of critical social issues, even after the investments of large amounts of resources in areas impacted by the construction of large hydropower dams. Our study focused on food insecurity and evaluated this issue in the city of Altamira in the Brazilian Amazon, which has been profoundly socially and economically impacted by the construction, between 2011 and 2015, of Brazil’s second-largest dam, namely, Belo Monte. A survey in Altamira city featured a 500-household random sample. Structural equation modeling showed conditioning factors of 60% of the population experiencing varying food insecurity degrees. Poverty, female-led households, lower education, youth, and unemployment were strongly linked to higher food insecurity. Crowded, officially impacted, and resettled households also faced heightened food insecurity. Our findings underscore the food insecurity conditions in the region impacted by the Belo Monte dam, emphasizing the need to take into account this crucial issue while planning and implementing hydropower dams.
Stress Due to Inflation: Changes over Time, Correlates, and Coping Strategies among Working-Age Adults in the United States
Authors: Mitra Sophie , Shen Chan , Pinnamraju Jahnavi , Wiener R. Constance , Wang Hao , Pathak Mona , Findley Patricia A. , Sambamoorthi Usha
Published: January 2024

Background: During the COVID-19 pandemic, the annual US inflation rate increased from 1.2% in July 2020 to 8% in July 2022. It has since declined to 3.4% (December 2023). This study examined the prevalence of stress due to inflation during a period when it declined from 8.2% in September 2022 to 3% in June 2023 and its association with demographic and social determinants of health (SDOH). Methods: We conducted a cross-sectional analysis of the online Household Pulse Survey (HPS), which surveils the experiences of US households. Beginning September 2022, HPS initiated data collection on “stress due to inflation” through a question on how stressful the increase in prices in the last 2 months has been. Participants could respond: very, moderately, a little, or not stressful. We analyzed data on working-age adults (18–64 years) who responded to the above question of stress due to inflation during 14–26 September 2022 (N = 32,579) and 7–19 June 2023 (N = 36,229). We used replicate weights in chi-squared tests and ordinal logistic regression analyses controlling for gender, age, race and ethnicity, COVID-19, COVID-19 vaccination, health insurance, and SDOH, including education, lost employment income, poverty status, marital status, food affordability, and region. Results: The prevalence of stress due to inflation (price increases being very or moderately stressful) significantly increased from 76.9% in September 2022 to 78.9% in June 2023. The odds of stress due to inflation were higher for individuals with the following characteristics: female, transgender, having income below 400% of the federal poverty line, having lost employment income, not being able to afford food, had long or acute COVID-19, and did not have a COVID-19 vaccine. Conclusions: More than three quarters of working-age adults in the US experienced stress due to inflation. Despite a declining national inflation rate in recent months, stress due to inflation has significantly increased among working-age adults. Inflation-related stress warrants further research and policy attention.
Psychosocial Impact of Infertility Diagnosis and Conformity to Gender Norms on the Quality of Life of Infertile Spanish Couples
Authors: Bueno-Sánchez Lidia , Alhambra-Borrás Tamara , Gallego-Valadés Alfonso , Garcés-Ferrer Jorge
Published: January 2024

Epidemiological data show that human reproductive disorders are a common problem worldwide, affecting almost one in six people of reproductive age. As a result, infertility has been identified by the World Health Organization as a public health disease. Reproductive problems can take a heavy toll on the psychosocial well-being of couples suffering from infertility. This is especially true for women, who tend to be the ones who undergo the most treatment. The main objective of the present study is to find out whether a sex-based infertility diagnosis influences the quality of life of couples with infertility. Also, we aim to find out whether the degree of adherence to gender norms influences their quality of life. A cross-sectional study was conducted using the Fertility Quality of Life Questionnaire (FertiQoL) and the Conformity to Feminine and Masculine Norms Inventories in a sample of 219 infertile Spanish couples (438 participants). The results show that, in all cases, regardless of the degree of conformity to gender norms and whether the infertility diagnosis was of female or male origin, women have lower scores on the self-perceived quality of life. This suggests that being female is already a psychosocial risk factor when assessing the psychosocial consequences of infertility.
Molecular Mechanisms of Seasonal Gene Expression in Trees
Authors: Chu Xian , Wang Minyan , Fan Zhengqi , Li Jiyuan , Yin Hengfu
Published: January 2024

In trees, the annual cycling of active and dormant states in buds is closely regulated by environmental factors, which are of primary significance to their productivity and survival. It has been found that the parallel or convergent evolution of molecular pathways that respond to day length or temperature can lead to the establishment of conserved periodic gene expression patterns. In recent years, it has been shown in many woody plants that change in annual rhythmic patterns of gene expression may underpin the adaptive evolution in forest trees. In this review, we summarize the progress on the molecular mechanisms of seasonal regulation on the processes of shoot growth, bud dormancy, and bud break in response to day length and temperature factors. We focus on seasonal expression patterns of genes involved in dormancy and their associated epigenetic modifications; the seasonal changes in the extent of modifications, such as DNA methylation, histone acetylation, and histone methylation, at dormancy-associated loci have been revealed for their actions on gene regulation. In addition, we provide an outlook on the direction of research on the annual cycle of tree growth under climate change.
PVA-Based Electrospun Materials—A Promising Route to Designing Nanofiber Mats with Desired Morphological Shape—A Review
Authors: Türkoğlu Gizem Ceylan , Khomarloo Niloufar , Mohsenzadeh Elham , Gospodinova Dilyana Nikolaeva , Neznakomova Margarita , Salaün Fabien
Published: January 2024

Poly(vinyl alcohol) is one of the most attractive polymers with a wide range of uses because of its water solubility, biocompatibility, low toxicity, good mechanical properties, and relatively low cost. This review article focuses on recent advances in poly(vinyl alcohol) electrospinning and summarizes parameters of the process (voltage, distance, flow rate, and collector), solution (molecular weight and concentration), and ambient (humidity and temperature) in order to comprehend the influence on the structural, mechanical, and chemical properties of poly(vinyl alcohol)-based electrospun matrices. The importance of poly(vinyl alcohol) electrospinning in biomedical applications is emphasized by exploring a literature review on biomedical applications including wound dressings, drug delivery, tissue engineering, and biosensors. The study also highlights a new promising area of particles formation through the electrospraying of poly(vinyl alcohol). The limitations and advantages of working with different poly(vinyl alcohol) matrices are reviewed, and some recommendations for the future are made to advance this field of study.
Extended Cleavage Specificity of two Hematopoietic Serine Proteases from a Ray-Finned Fish, the Spotted Gar (Lepisosteus oculatus)
Authors: Valentini Paolo , Akula Srinivas , Alvarado-Vazquez Abigail , Hallgren Jenny , Fu Zhirong , Racicot Brett , Braasch Ingo , Thorpe Michael , Hellman Lars
Published: January 2024

The extended cleavage specificities of two hematopoietic serine proteases originating from the ray-finned fish, the spotted gar (Lepisosteus oculatus), have been characterized using substrate phage display. The preference for particular amino acids at and surrounding the cleavage site was further validated using a panel of recombinant substrates. For one of the enzymes, the gar granzyme G, a strict preference for the aromatic amino acid Tyr was observed at the cleavable P1 position. Using a set of recombinant substrates showed that the gar granzyme G had a high selectivity for Tyr but a lower activity for cleaving after Phe but not after Trp. Instead, the second enzyme, gar DDN1, showed a high preference for Leu in the P1 position of substrates. This latter enzyme also showed a high preference for Pro in the P2 position and Arg in both P4 and P5 positions. The selectivity for the two Arg residues in positions P4 and P5 suggests a highly specific substrate selectivity of this enzyme. The screening of the gar proteome with the consensus sequences obtained by substrate phage display for these two proteases resulted in a very diverse set of potential targets. Due to this diversity, a clear candidate for a specific immune function of these two enzymes cannot yet be identified. Antisera developed against the recombinant gar enzymes were used to study their tissue distribution. Tissue sections from juvenile fish showed the expression of both proteases in cells in Peyer’s patch-like structures in the intestinal region, indicating they may be expressed in T or NK cells. However, due to the lack of antibodies to specific surface markers in the gar, it has not been possible to specify the exact cellular origin. A marked difference in abundance was observed for the two proteases where gar DDN1 was expressed at higher levels than gar granzyme G. However, both appear to be expressed in the same or similar cells, having a lymphocyte-like appearance.
Aquaporin-4 and Parkinson’s Disease
Authors: Lapshina Ksenia V. , Ekimova Irina V.
Published: January 2024

The water-selective channel aquaporin-4 (AQP4) is implicated in water homeostasis and the functioning of the glymphatic system, which eliminates various metabolites from the brain tissue, including amyloidogenic proteins. Misfolding of the α-synuclein protein and its post-translational modifications play a crucial role in the development of Parkinson’s disease (PD) and other synucleopathies, leading to the formation of cytotoxic oligomers and aggregates that cause neurodegeneration. Human and animal studies have shown an interconnection between AQP4 dysfunction and α-synuclein accumulation; however, the specific role of AQP4 in these mechanisms remains unclear. This review summarizes the current knowledge on the role of AQP4 dysfunction in the progression of α-synuclein pathology, considering the possible effects of AQP4 dysregulation on brain molecular mechanisms that can impact α-synuclein modification, accumulation and aggregation. It also highlights future directions that can help study the role of AQP4 in the functioning of the protective mechanisms of the brain during the development of PD and other neurodegenerative diseases.
New Discoveries on Protein Recruitment and Regulation during the Early Stages of the DNA Damage Response Pathways
Authors: Waters Kelly L. , Spratt Donald E.
Published: January 2024

Maintaining genomic stability and properly repairing damaged DNA is essential to staying healthy and preserving cellular homeostasis. The five major pathways involved in repairing eukaryotic DNA include base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), non-homologous end joining (NHEJ), and homologous recombination (HR). When these pathways do not properly repair damaged DNA, genomic stability is compromised and can contribute to diseases such as cancer. It is essential that the causes of DNA damage and the consequent repair pathways are fully understood, yet the initial recruitment and regulation of DNA damage response proteins remains unclear. In this review, the causes of DNA damage, the various mechanisms of DNA damage repair, and the current research regarding the early steps of each major pathway were investigated.
Neuroprotection via Carbon Monoxide Depends on the Circadian Regulation of CD36-Mediated Microglial Erythrophagocytosis in Hemorrhagic Stroke
Authors: Kaiser Sandra , Henrich Luise , Kiessling Iva , Loy Benedikt , Schallner Nils
Published: January 2024

The molecular basis for circadian dependency in stroke due to subarachnoid hemorrhagic stroke (SAH) remains unclear. We reasoned that microglial erythrophagocytosis, crucial for SAH response, follows a circadian pattern involving carbon monoxide (CO) and CD36 surface expression. The microglial BV-2 cell line and primary microglia (PMG) under a clocked medium change were exposed to blood ± CO (250 ppm, 1 h) in vitro. Circadian dependency and the involvement of CD36 were analyzed in PMG isolated from control mice and CD36−/− mice and by RNA interference targeting Per-2. In vivo investigations, including phagocytosis, vasospasm, microglia activation and spatial memory, were conducted in an SAH model using control and CD36−/− mice at different zeitgeber times (ZT). In vitro, the surface expression of CD36 and its dependency on CO and phagocytosis occurred with changed circadian gene expression. CD36−/− PMG exhibited altered circadian gene expression, phagocytosis and impaired responsiveness to CO. In vivo, control mice with SAH demonstrated circadian dependency in microglia activation, erythrophagocytosis and CO-mediated protection at ZT2, in contrast to CD36−/− mice. Our study indicates that circadian rhythmicity modulates microglial activation and subsequent CD36-dependent phagocytosis. CO altered circadian-dependent neuroprotection and CD36 induction, determining the functional outcome in a hemorrhagic stroke model. This study emphasizes how circadian rhythmicity influences neuronal damage after neurovascular events.
Changes in the Dentate Gyrus Gene Expression Profile Induced by Levetiracetam Treatment in Rats with Mesial Temporal Lobe Epilepsy
Authors: Diaz-Villegas Veronica , Pichardo-Macías Luz Adriana , Juárez-Méndez Sergio , Ignacio-Mejía Iván , Cárdenas-Rodríguez Noemí , Vargas-Hernández Marco Antonio , Mendoza-Torreblanca Julieta Griselda , Zamudio Sergio R.
Published: January 2024

Temporal lobe epilepsy (TLE) is one of the most common forms of focal epilepsy. Levetiracetam (LEV) is an antiepileptic drug whose mechanism of action at the genetic level has not been fully described. Therefore, the aim of the present work was to evaluate the relevant gene expression changes in the dentate gyrus (DG) of LEV-treated rats with pilocarpine-induced TLE. Whole-transcriptome microarrays were used to obtain the differential genetic profiles of control (CTRL), epileptic (EPI), and EPI rats treated for one week with LEV (EPI + LEV). Quantitative RT–qPCR was used to evaluate the RNA levels of the genes of interest. According to the results of the EPI vs. CTRL analysis, 685 genes were differentially expressed, 355 of which were underexpressed and 330 of which were overexpressed. According to the analysis of the EPI + LEV vs. EPI groups, 675 genes were differentially expressed, 477 of which were downregulated and 198 of which were upregulated. A total of 94 genes whose expression was altered by epilepsy and modified by LEV were identified. The RT–qPCR confirmed that LEV treatment reversed the increased expression of Hgf mRNA and decreased the expression of the Efcab1, Adam8, Slc24a1, and Serpinb1a genes in the DG. These results indicate that LEV could be involved in nonclassical mechanisms involved in Ca2+ homeostasis and the regulation of the mTOR pathway through Efcab1, Hgf, SLC24a1, Adam8, and Serpinb1a, contributing to reduced hyperexcitability in TLE patients.
In the Rat Hippocampus, Pilocarpine-Induced Status Epilepticus Is Associated with Reactive Glia and Concomitant Increased Expression of CD31, PDGFRβ, and Collagen IV in Endothelial Cells and Pericytes of the Blood–Brain Barrier
Authors: Kyriatzis Grigorios , Bernard Anne , Bôle Angélique , Khrestchatisky Michel , Ferhat Lotfi
Published: January 2024

In humans and animal models, temporal lobe epilepsy (TLE) is associated with reorganization of hippocampal neuronal networks, gliosis, neuroinflammation, and loss of integrity of the blood–brain barrier (BBB). More than 30% of epilepsies remain intractable, and characterization of the molecular mechanisms involved in BBB dysfunction is essential to the identification of new therapeutic strategies. In this work, we induced status epilepticus in rats through injection of the proconvulsant drug pilocarpine, which leads to TLE. Using RT-qPCR, double immunohistochemistry, and confocal imaging, we studied the regulation of reactive glia and vascular markers at different time points of epileptogenesis (latent phase—3, 7, and 14 days; chronic phase—1 and 3 months). In the hippocampus, increased expression of mRNA encoding the glial proteins GFAP and Iba1 confirmed neuroinflammatory status. We report for the first time the concomitant induction of the specific proteins CD31, PDGFRβ, and ColIV—which peak at the same time points as inflammation—in the endothelial cells, pericytes, and basement membrane of the BBB. The altered expression of these proteins occurs early in TLE, during the latent phase, suggesting that they could be associated with the early rupture and pathogenicity of the BBB that will contribute to the chronic phase of epilepsy.
A Blood Test for the Diagnosis of Multiple Sclerosis
Authors: Giuliano Paola , La Rosa Giuliana , Capozzi Serena , Cassano Emanuele , Damiano Simona , Habetswallner Francesco , Iodice Rosa , Marra Maurizio , Pavone Luigi Michele , Quarantelli Mario , Vitelli Giuseppe , Santillo Mariarosaria , Paternò Roberto
Published: January 2024

Multiple sclerosis (MS) is an autoimmune chronic disease characterized by inflammation and demyelination of the central nervous system (CNS). Despite numerous studies conducted, valid biomarkers enabling a definitive diagnosis of MS are not yet available. The aim of our study was to identify a marker from a blood sample to ease the diagnosis of MS. In this study, since there is evidence connecting the serotonin pathway to MS, we used an ELISA (Enzyme-Linked Immunosorbent Assay) to detect serum MS-specific auto-antibodies (auto-Ab) against the extracellular loop 1 (ECL-1) of the 5-hydroxytryptamine (5-HT) receptor subtype 2A (5-HT2A). We utilized an ELISA format employing poly-D-lysine as a pre-coating agent. The binding of 208 serum samples from controls, both healthy and pathological, and of 104 serum samples from relapsing–remitting MS (RRMS) patients was tested. We observed that the serum-binding activity in control cohort sera, including those with autoimmune and neurological diseases, was ten times lower compared to the RRMS patient cohort (p = 1.2 × 10−47), with a sensitivity and a specificity of 98% and 100%, respectively. These results show that in the serum of patients with MS there are auto-Ab against the serotonin receptor type 2A which can be successfully used in the diagnosis of MS due to their high sensitivity and specificity.
CRISPR Manipulation of Age-Related Macular Degeneration Haplotypes in the Complement System: Potential Future Therapeutic Applications/Avenues
Authors: Salman Ahmed , McClements Michelle E. , MacLaren Robert E.
Published: January 2024

Age-related macular degeneration (AMD) is the leading cause of irreversible vision loss among the elderly in the developed world. Whilst AMD is a multifactorial disease, the involvement of the complement system in its pathology is well documented, with single-nucleotide polymorphisms (SNPs) in different complement genes representing an increased risk factor. With several complement inhibitors explored in clinical trials showing limited success, patients with AMD are still without a reliable treatment option. This indicates that there is still a gap of knowledge in the functional implications and manipulation of the complement system in AMD, hindering the progress towards translational treatments. Since the discovery of the CRISPR/Cas system and its development into a powerful genome engineering tool, the field of molecular biology has been revolutionised. Genetic variants in the complement system have long been associated with an increased risk of AMD, and a variety of haplotypes have been identified to be predisposing/protective, with variation in complement genes believed to be the trigger for dysregulation of the cascade leading to inflammation. AMD-haplotypes (SNPs) alter specific aspects of the activation and regulation of the complement cascade, providing valuable insights into the pathogenic mechanisms of AMD with important diagnostic and therapeutic implications. The effect of targeting these AMD-related SNPs on the regulation of the complement cascade has been poorly explored, and the CRISPR/Cas system provides an ideal tool with which to explore this avenue. Current research concentrates on the association events of specific AMD-related SNPs in complement genes without looking into the effect of targeting these SNPs and therefore influencing the complement system in AMD pathogenesis. This review will explore the current understanding of manipulating the complement system in AMD pathogenesis utilising the genomic manipulation powers of the CRISPR/Cas systems. A number of AMD-related SNPs in different complement factor genes will be explored, with a particular emphasis on factor H (CFH), factor B (CFB), and complement C3 (C3).
Kynurenines, Neuronal Excitotoxicity, and Mitochondrial Oxidative Stress: Role of the Intestinal Flora
Authors: Nagy-Grócz Gábor , Spekker Eleonóra , Vécsei László
Published: January 2024

The intestinal flora has been the focus of numerous investigations recently, with inquiries not just into the gastrointestinal aspects but also the pathomechanism of other diseases such as nervous system disorders and mitochondrial diseases. Mitochondrial disorders are the most common type of inheritable metabolic illness caused by mutations of mitochondrial and nuclear DNA. Despite the intensive research, its diagnosis is usually difficult, and unfortunately, treating it challenges physicians. Metabolites of the kynurenine pathway are linked to many disorders, such as depression, schizophrenia, migraine, and also diseases associated with impaired mitochondrial function. The kynurenine pathway includes many substances, for instance kynurenic acid and quinolinic acid. In this review, we would like to show a possible link between the metabolites of the kynurenine pathway and mitochondrial stress in the context of intestinal flora. Furthermore, we summarize the possible markers of and future therapeutic options for the kynurenine pathway in excitotoxicity and mitochondrial oxidative stress.