bluuebunny/crossref_metadata_2025 huggingface dataset:
Inherent Safety Characteristics of Lead Bismuth Eutectic-Cooled Accelerator Driven Subcritical Systems
Authors: Luo Run , Revankar Shripad T. , Zhang De , Zhao Fuyu
Published: March 2022

An accelerator driven subcritical system (ADS) is a new nuclear energy system which could not only produce clean energy but also incinerate nuclear waste. In this paper, inherent safety analysis of an ADS is performed with neutronics and thermal-hydraulics coupled code named ARTAP. Five typical accidents are carried out, including the cases of proton beam interruption, transient overpower, reactivity insertion, loss of flow, and loss of heat sink. The transient simulations are performed in the average channel and the hottest channel of the fuel pin in the ADS core. The simulation results for beam interruption show that the highest temperature of the pellet is in the middle of the fuel element in the average channel, while the peak temperature of the cladding is in the top of the fuel element. After the beam is interrupted for 20s, the maximum temperature drops at the fuel center, the cladding inner surface, and the outlet coolant in the hottest channel are 644.46K, 162.27K, and 136.42K respectively. For transient overpower accidents with the increase of beam intensity, the maximum temperature of the fuel and the cladding are below the safety limit. Concerning the reactivity insertion accident, it is found that the ADS has good inherent safety and its margin of criticality safety is large. The calculation results for loss of flow show that the power drop is small due to low sensitivity of the subcritical core to negative reactivity feedback, and the maximum temperature of the cladding reaches 1726K, which means the fuel element would rupture. However, the power and the temperatures of fuel, cladding, and coolant could decrease quickly to the safety level after the proton accelerator is cut off under a loss of flow accident. The results also show that the peak temperature of the cladding is lower than the safety limit under a loss of heat sink accident. The present simulation results reveal that the ADS has a remarkable advantage against severe accidents. It also implies that its inherent safety characteristics could ensure reactor shutdown by cutting off the proton beam after accidents occur.
Environmental Regulation, Sentiment, and Eco-Innovation: Evidence From China
Authors: Sun Yunpeng , Li Haoning , Bao Qun , Lu Zhou
Published: March 2022

This study aims to shed light on the effects of environmental regulation on China’s eco-innovation driven by investor sentiment and public sentiment from 2003 to 2017. Adopting a text analysis technique and LSTM model, this study identifies the investor sentiment and public sentiment by online comments. We find that environmental regulation has a positive effect on China’s eco-innovation through public sentiment, whereas environmental regulation inhibits China’s eco-innovation through investor sentiment. Second, the regulatory tools of environmental regulation not only improve the public’s enthusiasm and awareness of environmental protection and eco-innovation in China but also bring the pressure to investor survival and continuous innovation. Third, we confirm that the environmental regulation has threshold effects on general public sentiment and investor sentiment for affecting China’s eco-innovation. Based on this result, environmental regulation easily triggers public sentiment for affecting China’s eco-innovation. Furthermore, in order to test whether environmental regulation has spatial spillover effect, by constructing the Spatial Durbin model, this study finds that the environmental regulation has spatial spillover effects on different regions’ eco-innovation in China through two kinds of sentiment. To the policy implication, this study from two kinds of sentiment to guide the environmental regulators would effectively improve the level of eco-innovation in China.
Soil Health: New Opportunities to Innovate in Crop Protection Research and Development
Authors: Atwood L. W. , Racette K. A. , Diggelmann M. , Masala C. A. , Maund S. , Oliver R. , Screpanti C. , Wironen M. , Wood S. A.
Published: March 2022

Soil health-based agricultural management practices are widely promoted to reduce erosion, increase nutrient use efficiency, improve soil structure, and sustain or increase yields. Pest and disease management are less frequently considered as components of a soil health management system. We present a framework for how the crop protection industry can advance soil health by developing systems of crop protection innovation that simultaneously target soil health outcomes, either through direct impact on soil or by enabling practices that promote soil health outcomes. Such an approach could lead to cross-sectoral, integrated agricultural solutions that achieve agronomic, environmental, and economic goals.
Improvement of Lipoplexes With a Sialic Acid Mimetic to Target the C1858T PTPN22 Variant for Immunotherapy in Endocrine Autoimmunity
Authors: Arena Andrea , Belcastro Eugenia , Ceccacci Francesca , Petrini Stefania , Conti Libenzio Adrian , Pagliarosi Olivia , Giorda Ezio , Sennato Simona , Schiaffini Riccardo , Wang Peng , Paulson James C. , Mancini Giovanna , Fierabracci Alessandra
Published: March 2022

The C1858T variant of the protein tyrosine phosphatase N22 (PTPN22) gene is associated with pathophysiological phenotypes in several autoimmune conditions, namely, Type 1 diabetes and autoimmune thyroiditis. The R620W variant protein, encoded by C1858T, leads to a gain of function mutation with paradoxical reduced T cell activation. We previously exploited a novel personalized immunotherapeutic approach based on siRNA delivered by liposomes (lipoplexes, LiposiRNA) that selectively inhibit variant allele expression. In this manuscript, we functionalize lipoplexes carrying siRNA for variant C1858T with a high affinity ligand of Siglec-10 (Sig10L) coupled to lipids resulting in lipoplexes (LiposiRNA-Sig10L) that enhance delivery to Siglec-10 expressing immunocytes. LiposiRNA-Sig10L lipoplexes more efficiently downregulated variant C1858T PTPN22 mRNA in PBMC of heterozygous patients than LiposiRNA without Sig10L. Following TCR engagement, LiposiRNA-Sig10L more significantly restored IL-2 secretion, known to be paradoxically reduced than in wild type patients, than unfunctionalized LiposiRNA in PBMC of heterozygous T1D patients.
Grapefruit Oil and Cobalt Nitrate-Loaded Polyurethane Hybrid Nanofibrous Scaffold for Biomedical Applications
Authors: Mani Mohan Prasath , Mohd Faudzi Ahmad Athif , Mohamaddan Shahrol , Ismail Ahmad Fauzi , Rathanasamy Rajasekar , Ayyar Manikandan , Jaganathan Saravana Kumar
Published: March 2022

The goal of this work is to fabricate a new composite based on polyurethane (PU), grapefruit (GP) oil, and cobalt nitrate [Co(NO3)2] using an electrospinning technique. Morphology results revealed the reduction in the fiber diameter of the composites compared to pristine PU control. The interaction of PU with GP and Co(NO3)2 was confirmed by hydrogen bond formation evident in infrared analysis. The fabricated PU/GP composites depicted a more hydrophobic behavior, while PU/GP/Co(NO3)2 showed a hydrophilic behavior than the pristine PU. Atomic force micrographs (AFM) revealed that the developed composites showed a decrease in the surface roughness (Ra) compared to PU. The addition of GP and Co(NO3)2 improved the mechanical strength of the pristine PU. The blood compatibility assays concluded not only the increase in blood clotting levels but also the less toxic nature of the fabricated composites compared to the pristine PU. Hence, the newly designed composites possessing outstanding physicochemical and biological properties may be used as a potential candidate for scaffolding in tissue engineering applications.
A Comparative Study on the Degradation of Alkali-Activated Slag/Fly Ash and Cement-Based Mortars in Phosphoric Acid
Authors: Ren Jie , Zhang Lihai , Zhu Yingcan , Li Zhenming , San Nicolas Rackel
Published: March 2022

This study compares the degradation behavior of the alkali-activated slag/fly ash (AASF) and ordinary Portland cement (OPC) mortars exposed to phosphoric acid with different pH values. The experimental results show that AASF mortars exhibit better resistance than OPC mortars against surface damage, although both systems get white deposits on the surface in phosphoric acid with a relatively high pH level. AASF mortars obtained lower mass loss than OPC mortars in phosphoric acid with pH at 2 and 3. The strength reduction in AASF mortars after immersion in phosphoric acid is more significant than that in OPC mortars. However, total degradation depth of AASF was smaller than that of OPC regardless of the pH of the acid solutions. Based on the experimental data, linear relationships were identified between the slope of degradation depth–mass loss curves and the Al/Si and Ca/Si ratios of the binders. This may indicate a new way to assess the degradation behavior of AASF and OPC based on their chemical compositions.
Eosinophilia and Lung Cancer: Analysis From Real-World Data and Mendelian Randomization Study
Authors: Wang Zhufeng , Chen Bigui , Fu Yu , Ou Changxing , Rong Qiuping , Kong Xuetao , Xu Wei , Deng Yangqing , Jiang Mei , Xie Jiaxing
Published: March 2022

Background and Objective

Growing evidence added to the results from observational studies of lung cancer patients exhibiting eosinophilia. However, whether eosinophils contributed to tumor immune surveillance or neoplastic evolution was unknown. This study aimed to analyze the causal association between eosinophilia and lung cancer.

Methods

The causal effect of eosinophil count on lung cancer from a genome-wide association study (GWAS) was investigated using the two-sample Mendelian randomization (MR) method. Secondary results according to different histological subtypes of lung cancer were also implemented. Meanwhile, we compared the measured levels of blood eosinophil counts among different subtypes of lung cancer from real-world data.

Results

The median absolute eosinophilic count (unit: 109/L) [median (min, max): Lung adenocarcinoma 0.7 (0.5, 15); Squamous cell lung cancer 0.7 (0.5, 1.3); Small cell lung cancer 0.7 (0.6, 1.3); p = 0.96] and the median eosinophil to leukocyte ratio [median (min, max): Lung adenocarcinoma 8.7% (2.1, 42.2%); Squamous cell lung cancer 9.3% (4.1, 17.7%); Small cell lung cancer 8.9% (5.1, 24.1%); p = 0.91] were similar among different histological subtypes of lung cancer. MR methods indicated that eosinophilia may provide 28% higher risk for squamous cell lung cancer in East Asian [Weighted median method: odds ratio (OR) = 1.28, 95% CI: 1.04–1.57, p = 0.02].

Conclusion

Our study suggested that eosinophilia may be a potential causal risk factor in the progression of squamous cell lung cancer in East Asian.
UPLC-Q-TOF-MS/MS Analysis of Seco-Sativene Sesquiterpenoids to Detect New and Bioactive Analogues From Plant Pathogen Bipolaris sorokiniana
Authors: Wang Yan-Duo , Yang Jian , Li Qi , Li Yuan-Yuan , Tan Xiang-Mei , Yao Si-Yang , Niu Shu-Bin , Deng Hui , Guo Lan-Ping , Ding Gang
Published: March 2022

Seco-sativene sesquiterpenoids are an important member of phytotoxins and plant growth regulators isolated from a narrow spectrum of fungi. In this report, eight seco-sativene sesquiterpenoids (1–8) were first analyzed using the UPLC-Q-TOF-MS/MS technique in positive mode, from which their mass fragmentation pathways were suggested. McLafferty rearrangement, 1,3-rearrangement, and neutral losses were considered to be the main fragmentation patterns for the [M+1]+ ions of 1–8. According to the structural features (of different substitutes at C-1, C-2, and C-13) in compounds 1–8, five subtypes (A–E) of seco-sativene were suggested, from which subtypes A, B/D, and E possessed the diagnostic daughter ions at m/z 175, 189, and 203, respectively, whereas subtype C had the characteristic daughter ion at m/z 187 in the UPLC-Q-TOF-MS/MS profiles. Based on the fragmentation patterns of 1–8, several known compounds (1–8) and two new analogues (9 and 10) were detected in the extract of plant pathogen fungus Bipolaris sorokiniana based on UPLC-Q-TOF-MS/MS analysis, of which 1, 2, 9, and 10 were then isolated and elucidated by NMR spectra. The UPLC-Q-TOF-MS/MS spectra of these two new compounds (9 and 10) were consistent with the fragmentation mechanisms of 1–8. Compound 1 displayed moderate antioxidant activities with IC50 of 0.90 and 1.97 mM for DPPH and ABTS+ scavenging capacity, respectively. The results demonstrated that seco-sativene sesquiterpenoids with the same subtypes possessed the same diagnostic daughter ions in the UPLC-Q-TOF-MS/MS profiles, which could contribute to structural characterization of seco-sativene sesquiterpenoids. Our results also further supported that UPLC-Q-TOF-MS/MS is a powerful and sensitive tool for dereplication and detection of new analogues from crude extracts of different biological origins.
Phylogeographic Assessment Reveals Geographic Sources of HIV-1 Dissemination Among Men Who Have Sex With Men in Kenya
Authors: Nduva George M. , Otieno Frederick , Kimani Joshua , McKinnon Lyle R. , Cholette Francois , Sandstrom Paul , Graham Susan M. , Price Matt A. , Smith Adrian D. , Bailey Robert C. , Hassan Amin S. , Esbjörnsson Joakim , Sanders Eduard J.
Published: March 2022

HIV-1 transmission dynamics involving men who have sex with men (MSM) in Africa are not well understood. We investigated the rates of HIV-1 transmission between MSM across three regions in Kenya: Coast, Nairobi, and Nyanza. We analyzed 372 HIV-1 partial pol sequences sampled during 2006–2019 from MSM in Coast (N = 178, 47.9%), Nairobi (N = 137, 36.8%), and Nyanza (N = 57, 15.3%) provinces in Kenya. Maximum-likelihood (ML) phylogenetics and Bayesian inference were used to determine HIV-1 clusters, evolutionary dynamics, and virus migration rates between geographic regions. HIV-1 sub-subtype A1 (72.0%) was most common followed by subtype D (11.0%), unique recombinant forms (8.9%), subtype C (5.9%), CRF 21A2D (0.8%), subtype G (0.8%), CRF 16A2D (0.3%), and subtype B (0.3%). Forty-six clusters (size range 2–20 sequences) were found—half (50.0%) of which had evidence of extensive HIV-1 mixing among different provinces. Data revealed an exponential increase in infections among MSM during the early-to-mid 2000s and stable or decreasing transmission dynamics in recent years (2017–2019). Phylogeographic inference showed significant (Bayes factor, BF > 3) HIV-1 dissemination from Coast to Nairobi and Nyanza provinces, and from Nairobi to Nyanza province. Strengthening HIV-1 prevention programs to MSM in geographic locations with higher HIV-1 prevalence among MSM (such as Coast and Nairobi) may reduce HIV-1 incidence among MSM in Kenya.
Lipoproteins Cause Bone Resorption in a Mouse Model of Staphylococcus aureus Septic Arthritis
Authors: Schultz Michelle , Mohammad Majd , Nguyen Minh-Thu , Hu Zhicheng , Jarneborn Anders , Wienken Carina M. , Froning Matti , Pullerits Rille , Ali Abukar , Hayen Heiko , Götz Friedrich , Jin Tao
Published: March 2022

Septic arthritis, most often caused by Staphylococcus aureus, is a rapidly progressive and destructive joint disease with substantial mortality and morbidity. Staphylococcus aureus lipoproteins (Lpps) are known to induce arthritis and bone destruction. Here, we aimed to investigate the bone resorptive effect of S. aureus Lpps in a murine arthritis model by intra-articular injection of purified S. aureus Lpps, synthetic lipopeptides, and live S. aureus strains. Analyses of the bone mineral density (BMD) of the distal femur bone were performed. Intra-articular injection of both live S. aureus and purified S. aureus Lpps were shown to significantly decrease total- and trabecular BMD. Liquid chromatography–mass spectrometry analyses revealed that the Lpps expressed by S. aureus SA113 strain contain both diacyl and triacyl lipid moieties. Interestingly, synthetic diacylated lipopeptide, Pam2CSK4, was more potent in inducing bone resorption than synthetic triacylated lipopeptide, Pam3CSK4. Modified lipoproteins lacking the lipid moiety were deprived of their bone resorptive abilities. Monocyte depletion by clodronate liposomes fully abrogated the bone resorptive capacity of S. aureus lipoproteins. Our data suggest that S. aureus Lpps induce bone resorption in locally-induced murine arthritis, an effect mediated by their lipid-moiety through monocytes/macrophages.
Whole-Genome and Transcriptome Sequencing-Based Characterization of Bacillus Cereus NR1 From Subtropical Marine Mangrove and Its Potential Role in Sulfur Metabolism
Authors: Kashif Muhammad , Lu Zhaomei , Sang Yimeng , Yan Bing , Shah Syed Jalil , Khan Sohail , Azhar Hussain Muhammad , Tang Hongzhen , Jiang Chengjian
Published: March 2022

Sulfur, organosulfur compounds, and sulfides are essential parts of life. Microbial sulfate assimilation is among the most active and ancient metabolic activities in the sulfur cycle that operates in various ecosystems. We analyzed the molecular basis of bacterial characterization. NR1 was isolated and purified from mangrove sediments. Whole-genome sequencing indicated that the NR1 isolate was closely related to Bacillus cereus. The genome contained 5,305 functional genes with a total length of 5,420,664 bp, a GC content of 35.62%, 42 rRNA, and 107 tRNA. DBT-grown cultures exhibited DBT utilization, fleeting emergence of DBT sulfone (DBTO2), and formation of 2-hydroxybiphenyl (2-HBP). Molecular analysis of the PCR products’ dsz operon revealed the presence of dszA, dszB, and dszC genes, which encoded for NR1’s 90% DBT desulfurization activity. Furthermore, 17 sulfur metabolism-related genes, including genes involved in assimilation sulfate reduction, APS and PAPS, and the cys, ssu, and TST gene families, were identified. In sulfate media, alkenesulfonate was converted to sulfite and inhibited ssu enzymes. Downregulated cysK variants were associated with nrnA expression and the regulation of L-cysteine synthesis. These findings established a scientific foundation for further research and application of bacteria to mangrove rehabilitation and ecological treatment by evaluating the bacterial characterization and sulfur degradation metabolic pathway. We used whole-genome and transcriptome sequencing to examine their genetic characteristics.
Past, Present, and Future of Deep Brain Stimulation: Hardware, Software, Imaging, Physiology and Novel Approaches
Authors: Frey Jessica , Cagle Jackson , Johnson Kara A. , Wong Joshua K. , Hilliard Justin D. , Butson Christopher R. , Okun Michael S. , de Hemptinne Coralie
Published: March 2022

Deep brain stimulation (DBS) has advanced treatment options for a variety of neurologic and neuropsychiatric conditions. As the technology for DBS continues to progress, treatment efficacy will continue to improve and disease indications will expand. Hardware advances such as longer-lasting batteries will reduce the frequency of battery replacement and segmented leads will facilitate improvements in the effectiveness of stimulation and have the potential to minimize stimulation side effects. Targeting advances such as specialized imaging sequences and “connectomics” will facilitate improved accuracy for lead positioning and trajectory planning. Software advances such as closed-loop stimulation and remote programming will enable DBS to be a more personalized and accessible technology. The future of DBS continues to be promising and holds the potential to further improve quality of life. In this review we will address the past, present and future of DBS.
Intravenous Lidocaine and Ketamine Infusions for Headache Disorders: A Retrospective Cohort Study
Authors: Ray Jason C. , Cheng Shuli , Tsan Kirsten , Hussain Hassan , Stark Richard J. , Matharu Manjit S. , Hutton Elspeth
Published: March 2022

Introduction

The use of lidocaine (lignocaine) and ketamine infusion in the inpatient treatment of patients with headache disorders is supported by small case series. We undertook a retrospective cohort study in order to assess the efficacy, duration and safety of lidocaine and ketamine infusions.

Methods

Patients admitted between 01/01/2018 and 31/07/2021 were identified by ICD code and electronic prescription. Efficacy of infusion was determined by reduction in visual analog score (VAS), and patient demographics were collected from review of the hospital electronic medical record.

Results

Through the study period, 83 infusions (50 lidocaine, 33 ketamine) were initiated for a headache disorder (77 migraine, three NDPH, two SUNCT, one cluster headache). In migraine, lidocaine infusion achieved a ≥50% reduction in pain in 51.1% over a mean 6.2 days (SD 2.4). Ketamine infusion was associated with a ≥50% reduction in pain in 34.4% over a mean 5.1 days (SD 1.5). Side effects were observed in 32 and 42.4% respectively. Infusion for medication overuse headache (MOH) led to successful withdrawal of analgesia in 61.1% of lidocaine, and 41.7% of ketamine infusions.

Conclusion

Lidocaine and ketamine infusions are an efficacious inpatient treatment for headache disorders, however associated with prolonged length-of-stay and possible side-effects.
Kernel Density Estimation of Electromyographic Signals and Ensemble Learning for Highly Accurate Classification of a Large Set of Hand/Wrist Motions
Authors: Ghaderi Parviz , Nosouhi Marjan , Jordanic Mislav , Marateb Hamid Reza , Mañanas Miguel Angel , Farina Dario
Published: March 2022

The performance of myoelectric control highly depends on the features extracted from surface electromyographic (sEMG) signals. We propose three new sEMG features based on the kernel density estimation. The trimmed mean of density (TMD), the entropy of density, and the trimmed mean absolute value of derivative density were computed for each sEMG channel. These features were tested for the classification of single tasks as well as of two tasks concurrently performed. For single tasks, correlation-based feature selection was used, and the features were then classified using linear discriminant analysis (LDA), non-linear support vector machines, and multi-layer perceptron. The eXtreme gradient boosting (XGBoost) classifier was used for the classification of two movements simultaneously performed. The second and third versions of the Ninapro dataset (conventional control) and Ameri’s movement dataset (simultaneous control) were used to test the proposed features. For the Ninapro dataset, the overall accuracy of LDA using the TMD feature was 98.99 ± 1.36% and 92.25 ± 9.48% for able-bodied and amputee subjects, respectively. Using ensemble learning of the three classifiers, the average macro and micro-F-score, macro recall, and precision on the validation sets were 98.23 ± 2.02, 98.32 ± 1.93, 98.32 ± 1.93, and 98.88 ± 1.31%, respectively, for the intact subjects. The movement misclassification percentage was 1.75 ± 1.73 and 3.44 ± 2.23 for the intact subjects and amputees. The proposed features were significantly correlated with the movement classes [Generalized Linear Model (GLM); P-value < 0.05]. An accurate online implementation of the proposed algorithm was also presented. For the simultaneous control, the overall accuracy was 99.71 ± 0.08 and 97.85 ± 0.10 for the XGBoost and LDA classifiers, respectively. The proposed features are thus promising for conventional and simultaneous myoelectric control.
Create Fat Substitute From Soybean Protein Isolate/Konjac Glucomannan: The Impact of the Protein and Polysaccharide Concentrations Formulations
Authors: Huang Lu , Ren Yuqing , Li He , Zhang Qibo , Wang Yong , Cao Jinnuo , Liu Xinqi
Published: March 2022

In this study, soybean protein isolate (SPI) and coconut oil were emulsified and konjac flour was added to prepare the protein/polysaccharide composite emulsion gel. The SPI/polysaccharide compound fat substitute was obtained by vacuuming. The effects of protein and konjac flour addition on the gel system of the mixed emulsion were explored. Sensory evaluation experiments showed that the overall acceptability of fat substitutes added with 1% SPI was higher. With the increase of protein and konjac content, the juiciness of the samples decreased gradually. The increase of konjac content reduced the brightness of compound fat substitutes, and the yellowness of compound fat substitute increases significantly with the increase of protein content. The rheological results showed that the G′ and loss modulus (G″) increased with the increase of protein and konjac content, forming a rigid elastic gel matrix, which provided a basis for the preparation of fat substitutes. Texture profile analysis (TPA) results showed that the springiness of all samples was similar to the natural fat after 20 min of heating. With the increase of protein and konjac content, the hardness of the samples increased gradually. The results of oral tribology showed that the friction coefficients of all samples were very small. The friction behavior of the samples with SPI content of 1% was similar to that of natural fat, which could better simulate the swallowing feeling and lubricity of natural fat. To sum up, the appearance of solid fat substitutes prepared with SPI and konjac flour is similar to pork fat. They show ideal functional characteristics in mechanical properties and oral tribology. Among them, the fat substitute with the protein content of 1% and konjac content of 4% is the most popular among consumers.
Production of a Novel Superoxide Dismutase by Escherichia coli and Pichia pastoris and Analysis of the Thermal Stability of the Enzyme
Authors: Zhao Yang , Zhao Liang , Zhang Weiwei , Rao Lei , Wang Yongtao , Liao Xiaojun
Published: March 2022

Previously, a new copper-zinc SOD (CuZnSOD) isolated from chestnut rose (Rosa roxburghii) with good stability was described. In this study, the biosynthetic approach was used to create recombinant CuZnSOD. RACE PCR was also used to amplify the full-length CuZnSOD gene from chestnut rose, and the ORF segment was expressed in E. coli BL21 and P. pastoris GS115. For characterization, the enzyme was isolated in two steps in E. coli and one step in P. pastoris. The biochemical properties of the two recombinant enzymes were similar, and their optimal reaction pH and temperature were 6.0 and 50°C, respectively. According to molecular dynamics simulation, the CuZnSOD showed high stability from 70 to 90°C, and eight amino acids are important for enzyme thermal stability at high temperatures. This study set the stage for industrial manufacture by filling gaps in the link between conformational changes and the thermal stability of the new CuZnSOD.
The Evolution of Clinicopathological Diagnostic Features of Upper Tract Urothelial Carcinoma in China: A Summary of 2561 Cases in the Last 20 Years
Authors: Xu Chunru , Yuan Changwei , Zhang Cuijian , Fang Dong , Yu Yanfei , Wang Xiang , Li Zhihua , Wang Yan , Tang Qi , Xiong Gengyan , Zhang Lei , He Zhisong , Lin Jian , Zhou Liqun , Li Xuesong
Published: March 2022

Objectives

To summarize the clinicopathological diagnostic features and evolutionary trends of upper tract urothelial carcinoma (UTUC) in China over the past 20 years.

Methods

All patients diagnosed with upper tract urothelial carcinoma in the Peking University First Hospital from 2001 to 2020 were retrospectively collected. Data were divided into two groups (2001-2010 and 2011-2020) according to the date of diagnosis. Statistical analysis was done with the SPSS V22.0. Chi-square analysis and t-test were adopted to analyze depending on the data type. Subgroup analysis based on 5 years was used for visualization to present trends. Both Kaplan-Meier curve and Cox regression were used for univariate and multivariate survival analysis.

Results

The study included 2561 cases diagnosed with upper tract urothelial carcinoma in total. Compared with the first decade (2001-2010), patients of the second decades (2011-2020) had elder mean age (66.65 versus 67.59, years, p=0.025), higher male proportion (43.5% versus 49.0%, p=0.034), lower incidence of renal pelvic tumors (53.4% versus 45.8%, p<0.001) and multifocality (18.6% versus 12.0%, p<0.001), higher incidence of ureteral tumors (52.2% versus 60.9%, p<0.001).In recent ten years, the incidence of muscle-invasive urothelial carcinoma (pT2+) decreased significantly (64.4% versus 54.9%, p<0.001),and the mean size of renal pelvic tumors increased(3.46 versus 3.73, cm, p=0.043). The size of the ureteral tumor, the histopathologic grade showed no significant change. The prognostic analysis based on 709 patients regularly followed at our center revealed that the male gender and G3 histopathological grade were independent risk factors for poorer prognosis in patients with UTUC.

Conclusion

In the past 20 years, the clinicopathological diagnostic features of upper tract urothelial carcinoma in the Chinese population has changed significantly, suggesting an increased risk of a poorer prognosis for UTUC. This trend may be related to updating diagnostic techniques and self-monitoring awareness. However, we need more high-grade, multicenter trials to verify it in the future.
Adipogenic Transdifferentiation and Regulatory Factors Promote the Progression and the Immunotherapy Response of Renal Cell Carcinoma: Insights From Integrative Analysis
Authors: Wang Shuai , Wei Xiyi , Ji Chengjian , Wang Yichun , Zhang Xi , Cong Rong , Song Ninghong
Published: March 2022

Background

Adipogenic transdifferentiation was an important carcinogenic factor in various tumors, while studies on its role in clear cell renal cell carcinoma (ccRCC) were still relatively few. This study aimed to investigate its prognostic value and mechanism of action in ccRCC.

Methods

Gene expression profiles and clinical data of ccRCC patients were obtained from The Cancer Genome Atlas database. Nonnegative matrix factorization was used for clustering. Gene set variation analysis (GSVA) and gene set enrichment analysis (GSEA) were used to analyze the pathways and biological process activities. single-sample GSEA (ssGSEA) was utilized to quantify the relative abundance of each immune cell. Tumor Immune Estimation Resource (TIMER) was used to evaluate the proportion of various immune infiltrating cells across diverse cancer types. Real-Time PCR was performed to examine the gene expression. R software was utilized to analyze the expression and prognostic role of genes in ccRCC.

Results

A total of 49 adipose-related genes (ARGs) were screened for differential expression between normal and ccRCC tissues. Based on differentially expressed ARGs, patients with ccRCC were divided into two adipose subtypes with different clinical, molecular, and pathway characteristics. Patients in cluster A exhibited more advanced pathological stages, higher expressions of RARRES2 and immune checkpoint genes, higher immune infiltration scores, and less nutrient metabolism pathways. Adipose differentiation index (ADI) was constructed according to the above ARGs and survival data, and its robustness and accuracy was validated in different cohorts. In addition, it was found that the expression of ARGs was associated with immune cell infiltration and immune checkpoint in ccRCC, among which GBP2 was thought to be the most relevant gene to the tumor immune microenvironment and play a potential role in carcinogenesis and invasion of tumor cells.

Conclusion

Our analysis revealed the consistency of higher adipogenic transdifferentiation of tumor cells with worse clinical outcomes in ccRCC. The 16-mRNA signature could predict the prognosis of ccRCC patients with high accuracy. ARGs such as GBP2 might shed light on the development of novel biomarkers and immunotherapies of ccRCC.
Case Report: Management of Recurrent Ovarian Squamous Cell Carcinoma With PD-1 Inhibitor
Authors: Song Xiao-chen , Wang Yong-xue , Yu Mei , Cao Dong-yan , Yang Jia-xin
Published: March 2022

Malignant transformations, such as ovarian squamous cell carcinoma (SCC) in ovarian mature cystic teratoma (OMCT), are rare tumors. The management of recurrent disease is still a challenge, and the gene mutations involved remain unclear. We herein report a recurrent case of ovarian SCC with a PIK3CA gene variation and immunohistochemical staining of programmed death-ligand 1 (PD-L1) >10%. This patient achieved clinical remission after platinum-based effective chemotherapy and programmed death 1 (PD-1) immunotherapy.
Vincristine, Irinotecan, and Temozolomide in Patients With Relapsed/Refractory Neuroblastoma
Authors: Zhu Jia , Wang Juan , Sun Feifei , Zhen Zijun , Chen Tingting , Lu Suying , Huang Junting , Zhang Yizhuo , Sun Xiaofei
Published: March 2022

Purpose

The combination of irinotecan, temozolomide and vincristine has been proposed as an effective salvage regimen for some pediatric malignancies. Thus, we sought to evaluate this combination for patients with relapsed and refractory neuroblastoma (NB).

Patients and Methods

In this retrospective study, forty-six patients with relapsed or refractory NB were treated with the combination of vincristine (1.5 mg/m2 i.v. day 1), irinotecan (50 mg/m2/day i.v. days 1–5) and temozolomide (100 mg/m2/day p.o. days 1–5) (VIT) during the period 2011–2019. All toxicities were documented.

Results

A total of 251 cycles (median 6 cycles/patient) were administered. A complete response (CR) was achieved in 5 patients, partial response (PR) in 27 patients, stable disease (SD) in 8 patients, and progression disease (PD) in 6 patients, with an overall objective response rate (CR+PR) of 69.6%. Eighteen patients developed diarrhea with Grade 3 or less. Grade 1-2 hematologic toxicity occurred in 10 patients. Grade 3-4 hematologic toxicity developed in 32 patients. VIT was an effective regimen for different metastatic sites. UGT1A*28 genotyping performed in 7 patients revealed wild type. Diarrhea occurred in 4 of them.

Conclusion

The shorter, 5-day VIT regimen is an active and well-tolerated salvage regimen in relapse/refractory NB.
New Liver MR Imaging Hallmarks for Small Hepatocellular Carcinoma Screening and Diagnosing in High-Risk Patients
Authors: Gao Feifei , Wei Yi , Zhang Tong , Jiang Hanyu , Li Qian , Yuan Yuan , Yao Shan , Ye Zheng , Wan Shang , Wei Xiaocheng , Nie Lisha , Tang Hehan , Song Bin
Published: March 2022

Objective

Early detection and diagnosis of hepatocellular carcinoma (HCC) is essential for prognosis; however, the imaging hallmarks for tumor detection and diagnosis has remained the same for years despite the use of many new immerging imaging methods. This study aimed to evaluate the detection performance of hepatic nodules in high risk patients using either hepatobiliary specific contrast (HBSC) agent or extracellular contrast agent (ECA), and further to compare the diagnostic performances for hepatocellular carcinoma (HCC) using different diagnostic criteria with the histopathological results as reference standard.

Methods

This prospective study included 247 nodules in 222 patients (mean age, 53.32 ± 10.84 years; range, 22–79 years). The detection performance and imaging features of each nodule were evaluated in all MR sequences by three experienced abdominal radiologists. The detection performance of each nodule on all MR sequences were compared and further the diagnostic performance of various diagnostic criteria were evaluated.

Results

For those patients who underwent ECA-MRI, the conventional imaging hallmark of “AP + PVP and/or DP” was recommended, as 60.19% diagnostic sensitivity, 80.95% specificity and 100% lesion detection rate. Additionally, for those patients who underwent HBSC-MRI, the diagnostic criteria of “DWI + HBP” was recommended. This diagnostic criteria demonstrated, both in all tumor size and for nodules ≤2 cm, higher sensitivity (93.07 and 90.16%, all p <0.05, respectively) and slightly lower specificity (64.71 and 87.50%, all p >0.05, respectively) than that of the European Association for the Study of the Liver (EASL) criteria.

Conclusions

Different abbreviated MR protocols were recommended for patients using either ECA or HBSC. These provided imaging settings demonstrated high lesion detection rate and diagnostic performance for HCC.
Updated Evaluation of the Diagnostic Performance of Double Contrast-Enhanced Ultrasonography in the Preoperative T Staging of Gastric Cancer: A Meta-Analysis and Systematic Review
Authors: Zhang Xin , Yao Jun , Zhang Yu , Huang Xin , Wang Weijun , Huang Hejing
Published: March 2022

Objective

This study aimed to systematically evaluate the diagnostic performance of double contrast-enhanced ultrasonography (DCEUS) in the preoperative T staging of gastric cancer (GC).

Methods

Literature searches for eligible studies were performed using MEDLINE, EMBASE, and Cochrane Library. The pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and area under the summary receiver operating characteristic curve of DCEUS in the diagnosis of each T stage tumor were calculated. Meta-analyses were performed to obtain the pooled effects of risk ratio (RR) with 95% confidence interval (CI) in the comparison of DCEUS with CT/endoscopic ultrasound (EUS).

Results

A total of 8 studies including 1,232 patients were identified for inclusion in this meta-analysis. The pooled sensitivity and specificity were 0.78 (95% CI = 0.64–0.88) and 0.98 (95% CI = 0.96–0.99) for T1, 0.81 (95% CI = 0.76–0.86) and 0.96 (95% CI = 0.91–0.98) for T2, 0.88 (95% CI = 0.84–0.91) and 0.85 (95% CI = 0.79–0.90) for T3, and 0.81 (95% CI = 0.69–0.89) and 0.96 (95% CI = 0.93–0.97) for T4. Moreover, DCEUS demonstrated significant superiority to CT in diagnosing T1 (RR = 1.57, 95% CI = 1.20–2.05, p = 0.001) and T2 (RR = 1.41, 95% CI = 1.16–1.71, p = 0.001) and to EUS in diagnosing T3 (RR = 1.24, 95% CI = 1.08–1.42, p = 0.003) and T4 (RR = 1.40, 95% CI = 1.09–1.79, p = 0.008). However, it showed a lower diagnostic accuracy than EUS in T1 tumors (RR = 0.77, 95% CI = 0.62–0.94, p = 0.013).

Conclusions

DCEUS is a feasible complementary diagnostic tool for clinical T staging of GC. However, it is still far from a definitive conclusion for DCEUS to be proposed for use in routine clinical practice.
Lymphatic Collecting Vessel: New Perspectives on Mechanisms of Contractile Regulation and Potential Lymphatic Contractile Pathways to Target in Obesity and Metabolic Diseases
Authors: Lee Yang , Zawieja Scott D. , Muthuchamy Mariappan
Published: March 2022

Obesity and metabolic syndrome pose a significant risk for developing cardiovascular disease and remain a critical healthcare challenge. Given the lymphatic system’s role as a nexus for lipid absorption, immune cell trafficking, interstitial fluid and macromolecule homeostasis maintenance, the impact of obesity and metabolic disease on lymphatic function is a burgeoning field in lymphatic research. Work over the past decade has progressed from the association of an obese phenotype with Prox1 haploinsufficiency and the identification of obesity as a risk factor for lymphedema to consistent findings of lymphatic collecting vessel dysfunction across multiple metabolic disease models and organisms and characterization of obesity-induced lymphedema in the morbidly obese. Critically, recent findings have suggested that restoration of lymphatic function can also ameliorate obesity and insulin resistance, positing lymphatic targeted therapies as relevant pharmacological interventions. There remain, however, significant gaps in our understanding of lymphatic collecting vessel function, particularly the mechanisms that regulate the spontaneous contractile activity required for active lymph propulsion and lymph return in humans. In this article, we will review the current findings on lymphatic architecture and collecting vessel function, including recent advances in the ionic basis of lymphatic muscle contractile activity. We will then discuss lymphatic dysfunction observed with metabolic disruption and potential pathways to target with pharmacological approaches to improve lymphatic collecting vessel function.
Mapping of QTLs for Yield Traits Using F2:3:4 Populations Derived From Two Alien Introgression Lines Reveals qTGW8.1 as a Consistent QTL for Grain Weight From Oryza nivara
Authors: Beerelli Kavitha , Balakrishnan Divya , Addanki Krishnam Raju , Surapaneni Malathi , Rao Yadavalli Venkateswara , Neelamraju Sarla
Published: March 2022

Wild introgressions play a crucial role in crop improvement by transferring important novel alleles and broadening allelic diversity of cultivated germplasm. In this study, two stable backcross alien introgression lines 166s and 14s derived from Swarn/Oryza nivara IRGC81848 were used as parents to generate populations to map quantitative trait loci (QTLs) for yield-related traits. Field evaluation of yield-related traits in F2, F3, and F4 population was carried out in normal irrigated conditions during the wet season of 2015 and dry seasons of 2016 and 2018, respectively. Plant height, tiller number, productive tiller number, total dry matter, and harvest index showed a highly significant association to single plant yield in F2, F3, and F4. In all, 21, 30, and 17 QTLs were identified in F2, F2:3, and F2:4, respectively, for yield-related traits. QTLs qPH6.1 with 12.54% phenotypic variance (PV) in F2, qPH1.1 with 13.01% PV, qTN6.1 with 10.08% PV in F2:3, and qTGW6.1 with 15.19% PV in F2:4 were identified as major effect QTLs. QTLs qSPY4.1 and qSPY6.1 were detected for grain yield in F2 and F2:3 with PV 8.5 and 6.7%, respectively. The trait enhancing alleles of QTLs qSPY4.1, qSPY6.1, qPH1.1, qTGW6.1, qTGW8.1, qGN4.1, and qTDM5.1 were from O. nivara. QTLs of the yield contributing traits were found clustered in the same chromosomal region. qTGW8.1 was identified in a 2.6 Mb region between RM3480 and RM3452 in all three generations with PV 6.1 to 9.8%. This stable and consistent qTGW8.1 allele from O. nivara can be fine mapped for identification of causal genes. From this population, lines C212, C2124, C2128, and C2143 were identified with significantly higher SPY and C2103, C2116, and C2117 had consistently higher thousand-grain weight values than both the parents and Swarna across the generations and are useful in gene discovery for target traits and further crop improvement.
Effects of Nitrogen and Phosphorus Addition on Soil Extracellular Enzyme Activity and Stoichiometry in Chinese Fir (Cunninghamia lanceolata) Forests
Authors: Liu Meihua , Gan Bingping , Li Quan , Xiao Wenfa , Song Xinzhang
Published: March 2022

Soil extracellular enzymes play an important role in microbial functions and soil nutrient cycling in the context of increasing N deposition globally. This is particularly important for Chinese fir (Cunninghamia lanceolata) forests because of the decline in soil fertility induced by successive rotation. In this study, we aimed to determine the effects of simulated N deposition (N30: 30 kg ha−2 year−1; N60: 60 kg ha−2 year−1) and phosphorus addition (P20: 20 mg kg−1; P40: 40 mg kg−1) on the activity and stoichiometry of soil extracellular enzymes related to soil C, N, and P cycling in Chinese fir. The results showed that N addition alone increased the activity of soil β-1,4 glucosidase (BG) but decreased the activity of N-acetyl-β-d-glucosidase (NAG) and leucine aminopeptidase (LAP). N addition increased the ratios of soil enzymes, C:N and C:P, alleviated microbial N-limitation, and aggravated microbial C-limitation. P addition alone increased enzyme activity, and P40 addition increased the ratio of BG to soil microbial biomass carbon (MBC), and (NAG + LAP):MBC activity ratio, thereby aggravating C restriction. N and P co-addition significantly affected soil extracellular enzyme activity and stoichiometry. For instance, BG activity and BG:MBC activity ratio increased significantly under the N30 + P40 treatment, which intensified C-limitation. Soil pH was the main factor influencing enzyme activity, and these variables were positively correlated. The stoichiometric relationships of enzyme reactions were coupled with soil pH, total nitrogen (TN), and available phosphorus (AP). Our results indicate that changes in soil characteristics induced by N and P inputs influence the activities of soil microorganisms and result in changes in microbial resource acquisition strategies. This study provides useful insights into the development of management strategies to improve the productivity of Chinese fir forests under scenarios of increasing N deposition.
Affect and Cognition in Managerial Decision Making: A Systematic Literature Review of Neuroscience Evidence
Authors: Cristofaro Matteo , Giardino Pier Luigi , Malizia Andrea P. , Mastrogiorgio Antonio
Published: March 2022

How do affect and cognition interact in managerial decision making? Over the last decades, scholars have investigated how managers make decisions. However, what remains largely unknown is the interplay of affective states and cognition during the decision-making process. We offer a systematization of the contributions produced on the role of affect and cognition in managerial decision making by considering the recent cross-fertilization of management studies with the neuroscience domain. We implement a Systematic Literature Review of 23 selected contributions dealing with the role of affect and cognition in managerial decisions that adopted neuroscience techniques/points of view. Collected papers have been analyzed by considering the so-called reflexive (X-) and reflective (C-) systems in social cognitive neuroscience and the type of decisions investigated in the literature. Results obtained help to support an emerging “unified” mind processing theory for which the two systems of our mind are not in conflict and for which affective states have a driving role toward cognition. A research agenda for future studies is provided to scholars who are interested in advancing the investigation of affect and cognition in managerial decision making, also through neuroscience techniques – with the consideration that these works should be at the service of the behavioral strategy field.
Psychometric Properties of the Zarit Burden Interview in Informal Caregivers of Persons With Intellectual Disabilities
Authors: Boluarte-Carbajal Alicia , Paredes-Angeles Rubí , Tafur-Mendoza Arnold Alejandro
Published: March 2022

Intellectual disability leads to a loss of autonomy and a high level of dependence, requiring support from another person permanently. Therefore, it is necessary to incorporate the assessment of caregiver burden in healthcare actions, to avoid putting the health of caregivers and patients at risk. In this sense, the study aimed to analyze the internal structure of the Zarit Burden Interview (ZBI) in a sample of caregivers of people with intellectual disabilities, to provide convergent and discriminant evidence with a measure of the risk of maltreatment, and to estimate the reliability of the scores from the Classical Test Theory and the Rasch Measurement Theory. The study was instrumental. The sample consisted of 287 Peruvian informal primary caregivers of persons diagnosed with intellectual disabilities. To collect validity evidence, the internal structure (confirmatory factor analysis, CFA) and the relationship with other variables (convergent and discriminant evidence) were used, while reliability was estimated through the omega coefficient and Rasch analysis. The internal structure of the ZBI corroborated a unidimensional structure. In terms of convergent and discriminant evidence, the scale presents adequate evidence. Reliability levels were also good. Previously, the psychometric properties of the ZBI have not been studied in caregivers of people with intellectual disabilities, and it represents the first study of the scale in Peru. The results obtained will allow the use of this scale to design actions in the work with caregivers and studies to understand the psychology of the caregiver.