bluuebunny/crossref_metadata_2025 huggingface dataset:
THE EFFECT OF LEARNING DISCIPLINE ON MATHEMATICS LEARNING OUTCOMES
Authors: Utama Ilham Tresna , Sukmanasa Elly , Wijaya Ade
Published: August 2022

The purpose of this study is to find outThe Effect of Learning Discipline on Mathematics Learning Outcomes. The population in this study were students of fourth grade of SDN 01Gunung Picung. The analysis prerequisite testing technique is in the form of a normality test, then the homogeneity test is carried out. Data that is declared normal and homogeneous is used to test the hypothesis which results show that there isThe Effect of Learning Discipline on Mathematics Learning Outcomes. Simple correlation regression analysis technique produces a relationship model which is expressed in the form of a regression equation, namelyŶ = 88.1 + 1.37X. The results of this study are indicated by statistical analysis which produces a correlation coefficient (rxy) of 0.57. While the coefficient of determination is 32.49%. Regression analysis techniques and simple correlation of learning discipline with mathematics learning outcomes produce an effect which is expressed in the form of a regression equation, namely Ŷ = 88.1 + 1.37X. which means that each increase in learning discipline units causes an increase in mathematics learning outcomes by 0.57 units. Based on the results of the research above, it can be concluded that there is a positive influence between the learning discipline and mathematics learning outcomes on the fourth grade of SDN 01 Gunung Picung, Bogor Regency, 2020/2021 academic year
THE INFLUENCE OF UNDERSTANDING OF ACADEMIC RULES ON THE STUDENTS CONFLICT
Authors: Muhajir Muhajir , Rismawati Rismawati , Latief Abdul
Published: August 2022

This research about of understanding of academic rules which aimed to the describe the understanding the students of Muhammadiyah University of Makassar toward academic rules and to describe the influence of the students' understanding of academic rules and the occurrence of conflict between students in Muhammadiyah University of Makassar. This research is ex-post facto research, also known as a retrospective study because this research is a research of returning to an event or an event and then tracing back to know the factors that can cause the incident. Based on data analysis, it can be concluded that (1) students’ understanding of academic rules and perceptions about the conflict with the occurrence of conflict between students at the Muhammadiyah University of Makassar are in the medium category, (2) There is a positive influence on the students' understanding of academic rules with the occurrence of conflict between students at the Muhammadiyah University of Makassar
SURVEY ON THE EXISTENCE OF THE INFORMAL SECTOR DURING THE COVID-19 PANDEMIC (Case Study In Mataram City, West Nusa Tenggara Province)
Authors: Suprianto Suprianto , Agustiani Eka , Alwi Mohammad
Published: August 2022

The purpose of this research is to The purpose of the study was to analyze the income and welfare level of informal sector workers during the Covid-19 pandemic in the city of Mataram. The research method was carried out by means of a survey by taking a sample of 60 respondents, and as many respondents as possible were determined by chance by looking at the characteristics of the informal sector workers. The data collected will be analyzed quantitatively and qualitatively. Qualitative analysis is used to explain or explain data from field research as a support for quantitative analysis.  The characteristics of informal and formal workers mostly sell in crowded places with dense population and visitors in the area of Mataram city seen from the level of education 56.7% have education equivalent to high school and above and 43.3% have graduated from junior high and elementary school with an average the average length of work is more than 10 hours a day, while the average family dependents for informal and formal workers are between 2 and 4 dependents. The average income earned by informal workers is below Rp 2,000,000, - as many as 25 respondents, 41.67% and income above Rp 2,000,000, - for 20 respondents or 33.33%. While the income of informal workers on average is above IDR 3,000,000, as many as 11 respondents, 18.33%, the rest of the income above IDR 5,000,000, as many as 4 respondents or 6.67% is obtained by fried rice traders and food stalls.total income analysis of informal workers if according to the Sajogyo criteria level with an equivalent size, then the poor category is 68.33% with an expenditure / capita / year of 450 kg - 650 kg equivalent to the value of rice / year. While the remaining 31.67% or as many as 19 respondents are at the threshold of affluent living with per capita expenditures / year above 750 kg as many as 9 respondents. While the decent living category if the competition per capita / year is higher than 960 kg equivalent to the value of rice / year as many as 10 respondents or 16.67%. Overall, the income received by informal workers during the Covid-19 pandemic decreased by 40% compared to before the Covid-19 pandemic.  To further increase the income of informal workers, in particular, to increase the working time of at least 15 hours per day and to open sales services through social media (medsos) with a digital system via the internet with Go food and Go-jek services, especially for traders of cooked food such as restaurants, fresh vegetables, rice and beverage traders.
CONFLICT RESOLUTION COUNSELING FOR A PEACEFUL FAMILY
Authors: Subkhan Muhamad , Maftuf Bunyamin , Malihah Elly , Rumiati Sri , Kurniati Pat
Published: August 2022

Humans are identical with a difference, because Allah Azza Wajalla created humans in different conditions. In fact, this difference has become a decree of Allah (Sunnatullah). On the other hand, humans are also given the nature of having a feeling of attraction to other types, especially when they hit puberty or adolescence. The feeling of attraction is the beginning of the feeling of belonging or the feeling of love. So for those who have reached a mature or mature age, they will focus their love on the desire to build a family or a household. Conflict in a household is a reality that cannot be avoided. Conflict can have a positive or negative impact. So managing conflict requires maturity to think. However, not all families are able to resolve their conflicts, therefore counseling is needed in resolving a conflict in the family, so that a peaceful family can be created or we are left with a sakinah mawadah warohmah family.
THE CONCEPT OF THE HUMAN BODY IN THE HOLY AL QUR'AN AND ITS IMPLICATIONS AGAINST ISLAMIC EDUCATION
Authors: Latipah Euis , Suhartini Andewi , EQ Nurwadjah Ahmad
Published: August 2022

Humans are creatures that Allah SWT created perfectly from the essence of the soil, then become nuthfah, then become mudzgoh and become bones wrapped in meat to form the human body. With this perfection, the human task is always to be grateful and to maintain this potential. Al-Qur'an invites people to think about the origin of humans about their creation. Because in the human body there is a sign of great power that shows the majesty of Allah SWT. The research method in this journal uses a type of qualitative research with a literature approach. The data was collected by searching related reference book sources. After the data is obtained then the data is analyzed and some additions from the researcher. In this study, researchers found several results and conclusions in the discussion of the human body in the Koran, that the verses of Allah on the human body are very clearly explained in the Koran, which at that time had no biological research. Second, the concept of the human body has implications for Islamic education.
EDUCATIONAL ENVIRONMENT IN THE IMPLEMENTATION OF CHARACTER EDUCATION
Authors: Suchyadi Yudhie , Suryani Amalia
Published: August 2022

the meaning and role of the environmenteducation in character education. The analytical method used indiscussion of the main topic using an effectual causal analysis model withexamines rational relationships, which analyzes the cause-and-effect relationships betweeneducational environment on character education with the main source ofliterature review. The results of the discussion show that the educational environmenthave a great influence on character education. This article concludesthat the implementation of character education needs to be supported by the environmentgood education
IMPLEMENTATION OF PUBLIC PARTICIPATION IN THE ESTABLISHMENT OF RULES OF LAWS AND REGULATIONS IN LEGISLATURE THE HOUSE OF REPRESENTATIVES OF THE REPUBLIC OF INDONESIA DURING THE COVID 19 PANDEMIC
Authors: Ikhwanudin Ikhwanudin , Tuti Retnowati WD
Published: August 2022

In the preamble to the Constitution of the Republic of Indonesia, it is stated that one of the objectives of the establishment of the Government of the State of Indonesia is to educate the life of the nation. Educating the nation's life through the education of people's sovereignty is one of the demands of a state of law. Realizing people's sovereignty in the Indonesian constitutional system is implemented through the branches of state power regulated in the Constitution of the Republic of Indonesia. The House Of Representatives (DPR), the Regional Representative Board (DPD), and the President hold the sovereign power of the people respectively. The DPR has the power to make laws. As a branch of state power that has the power to form laws, the DPR must pay attention to the sovereignty of the people which is reflected in the participation of the community or the public in forming laws and regulations, under any conditions, including during the Covid-19 pandemic. The involvement of public participation in legislation is intended so that the legislative products formed by the DPR are in accordance with the principles of people's sovereignty, aspirations and legal needs of the people and carry out the constitutional mandate, namely to educate the life of the nation. The implementation of public participation is not only carried out in the formation of laws and regulations, but also to the supervision of laws, as part of public political education efforts. Thus, one of the objectives of the formation of laws and regulations is to maintain the sovereignty of the people by educating the nation's life, even during the Covid 19 pandemic. This study aims to describe the implementation and objectives of public participation in the formation of legislation in the future. Covid-19 pandemic.
THE CULTURE AND LOCAL WISDOM OF THE INDIGENOUS PEOPLE KASEPUHAN SINAR RESMI
Authors: Pudjiastuti Sri Rahayu , Hadi Nestiyanto , Arfani Hamdan , Akbar Reza Purwana , Tajudin Amran Setia
Published: August 2022

The indigenous people of Kasepuhan Sinar Resmi are one of the indigenous communities that are members of the Banten Kidul traditional unit, which is located in the Sukabumi district, West Java. This study aims to: Identify the living conditions and their philosophies, social and cultural conditions of the people of Kasepuhan Sinar Resmi and Identify the local wisdom of the Kasepuhan Sinar Resmi residents and their implementation in daily life. To study the local wisdom values of the Kasepuhan Sinar Resmi, using a descriptive-qualitative method by conducting analysis and literature review. This study found that local cultural values serve as the basis for the daily life of the Kasepuhan indigenous people. As a farming community, Kasepuhan residents obey the rules of their ancestors by carrying out various traditional rituals related to agricultural activities. The philosophy of life of the Kasepuhan indigenous people, in treating nature, is a form of local wisdom that is maintained by the Kasepuhan indigenous people. The development of technology and information may affect the sustainability of the cultural values of the Kasepuhan indigenous people. The results of this study are expected to provide benefits in the form of: Understanding the philosophy of life as the basis for local wisdom of the community; and As a consideration in determining policies related to indigenous peoples.
COMMUNICATION STRATEGY FOR THE DEVELOPMENT OF ALMOUS ECOTURISM IN THE ISLAND OF THE KELAPA ISLAND
Authors: Muslim Muslim
Published: August 2022

This study aims to explore the perceptions of the community and tourists on the development plan for the marine alms ecotourism in the island of kelapa, and to formulate a communication strategy policy for the development of ecotourism in the Kelapa Island village. The approach used is a combination of communicative and qualitative approaches. Data analysis was compiled using tabulation, then interpreted descriptively qualitatively in accordance with the research objectives. The communication strategy implementation plan is carried out through a SWOT analysis. The perception of the village community to participate in its management so as to increase business opportunities and income. In addition, it can preserve rituals as a habitual action (hereditary action) and also contain transcendental values while maintaining natural preservation. The perception of visitors to the existence of sea alms strongly supports the ritual of "Sea Alms" which is used as a tourist attraction for marine culture, by displaying dance, culinary arts and typical Bugis cultural symbols which are ornaments of marine tourism attractions. Recommendations for ecotourism development communication strategies are: (1). Creating seaside village branding (2) conducting intensive promotions (3) Synergizing ecotourism with local culture and wisdom (4) Empowering local communities in ecotourism activities.
THE EFFECTS OF COORDINATION ON PUBLIC SERVICE QUALITY AT THE OFFICE OF ARCHIVES AND LIBRARY OF SUMEDANG REGENCY
Authors: Hermayanty Irma
Published: August 2022

This study aims to determine the effects of Coordination on the Public Service Quality at the Office of Archives and Library of Sumedang Regency and to determine the relationship between Coordination and Public Services Quality at the Office of Archives and Library of Sumedang Regency. The method used in this study is a quantitative research method to test the predetermined hypothesis. In this study, researchers used inferential descriptive data analysis techniques to describe the collected data as it is by making general conclusions. The sampling technique used in this study is a saturated sampling technique with a population of 70 people to obtain a sample size of 70 respondents.Based on the results of the study, it can be illustrated that the coordination at the Office of Archives and Library of Sumedang Regency is sufficient, reaching 67.80%, and the Public Service Quality at the Office of Archives and Library of Sumedang Regency reaches 65.70%, and this figure is categorized as sufficient. The correlation between coordination and public service quality at the Office of Archives and Library of Sumedang Regency with a correlation coefficient of 0.52 is in the medium category. Meanwhile, the effect of coordination public service quality at the Office of Archives and Library of Sumedang Regency reached 27%, while the remaining 73% was affected by unexamined factors called the epsilon factor.
THE EFFECT OF EDMODO – BASED BLENDED LEARNING ON ACCOUNTING LEARNING OUTCOMES VIEWED SELF REGULATED LEARNING
Authors: Satiti Abidah Dwi Rahmi , Fibriyanti Yenni Vera
Published: August 2022

The objective of this research was to investigate the difference effect between the Edmodo - Based Blended Learning and the conventional learning on accounting learning outcomes viewed self regulated learning. This research was an quasi experimental with nonequivalent pretest posttest control-group design. The population of research was the students in semester 4 of classes A, B, and C. The research sample was taken 2 classes, namely class B and class C.  The data were analyzed by using the two-way analysis of variance and was continued with the Scheffe test. The findings showed that: (1) there was a difference effect between the Edmodo –Based Blended Learning on accounting learning outcomes; (2) there was a difference effect of the high, moderate, and low self regulated learning on accounting learning outcomes; and (3) there was not an interaction between the Edmodo – Based Blended Learning and the conventional learning with self regulated learning on accounting learning outcomes.
INCREASING COMMITMENT TO ORGANIZATION THROUGH ORGANIZATIONAL JUSTICE AND GROUP COHESIVENESS (Study Using Correlation Approach and SITOREM Analysis on Private Junior High School Teachers in Tanah Sareal District, Bogor City)
Authors: Patras Yuyun Elizabeth , Hendarman Hendarman , Kuswendi Uut
Published: August 2022

Commitment to organization is determined by the extent to which organizational justice and group cohesiveness emerges and manifests within the organization. In the context of school, this is a challenge that must be used as a principle in the development and performance change for each element in school. The purpose of this study is to seek efforts to increase commitment to the organization through an assessment of the strength of the relationship between commitment to organization owned by teachers and organizational justice and group cohesiveness. This study was conducted with a quantitative approach using correlation analysis and SITOREM analysis. The research was conducted in 16 private junior high schools in Tanah Sareal district, Bogor City. The research population was 171 teachers, the research sample was 120 permanent teachers’ foundation the so-called Gru Tetap Yayasan (GTY). The results of this study are: 1) there is a very significant positive relationship between organizational justice and commitment to organization; 2) there is a very significant positive relationship between group cohesiveness and commitment to organization; 3) there is a very significant positive relationship between organizational justice and group cohesiveness together with commitment to organization. Efforts that need to be improved to increase commitment to the organization are: 1) honesty, 2) feasibility in obtaining resources, 3) propriety, 4) justification, 5) explanation, 6) process control, 7) intensity of members to be together and 9) loyalty towards school.
THE INFLUENCE OF SOFT SKILLS AND E-COMMERCE DEVELOPMENT ACTIVITIES ON INCREASING INCOME DURING THE COVID-19 PANDEMIC IN THE WOMEN'S BUSINESS GROUP IN JATIREJO VILLAGE
Authors: Najiah Evi Fitrotun , Mahmudah Henny , Agustin Niken
Published: August 2022

The Jatirejo village government during the Covd-19 pandemic sees the need for soft skills and e-commerce development activities that are intended primarily for residents/communities. This is considered very useful in increasing the income of the people in Jatirejo village during the Covid-19 pandemic. In this activity, the village government held training for people who have businesses and people who are unemployed as a result of the impact of the pandemic in hamlets such as Topeng Hamlet, Kemendung Hamlet, Karangpilang Hamlet and Tambakjurit Hamlet.The purpose of this study was to determine whether soft skills and E-Commerce, either partially or simultaneously, had an effect on increasing income during the Covid 19 pandemic in the women's business group in Jatirejo Village. In this research, quantitative descriptive method is used by looking at the impact of the activities that have been carried out on increasing income. This activity was started first with observations and interviews, then continued with multiple linear regression testing, Partial Hypothesis (t-test) and Simultaneous Hypothesis (F-Test) using the SPSS tool.The results of this study obtained Multiple Linear Regression test Y = 8.702 + 0.241X1 +0.732X2 , Partial Hypothesis Test t obtained t value (soft skill) 2.105; the value of t arithmetic (E-Commerce) 4.271 is greater than the value of t table 2.032 with = 0.05. significance value 0.000 0.05, the results of the Simultaneous Hypothesis test obtained Fcount 33.351 F table 3.28 with = 0.05. significance value 0.000 0.05. Then Ho is rejected and H1 is accepted.From the results of this study, it can be concluded that soft skills and E-Commerce, both partially and simultaneously, have an effect on increasing income during the Covid 19 pandemic in the Jatirejo Village women's business group and the most dominant influencing variable is E-Commerce.
ALTERNATIVE MARKETING STRATEGIES OF TRADITIONAL DRUG IN FACING THE NEW NORMAL ERA AS THE IMPACT OF COVID 19
Authors: Yulia Evi , Nurcholidah Lilik , Sari Kurnia Riska
Published: August 2022

The purpose of this research is to find out alternative marketing strategies for traditional medicine in the face of the new normal era as the impact of the second covid 19 to find out the best alternative strategy that can be used for traditional medicine in facing the new normal era as the impact of covid 19. This type of research is qualitative with tools The analysis used is a SWOT analysis. The results of the study indicate that an alternative strategy that can be used is the SO Strategy, one of which is by conducting or increasing the marketing of herbal medicines by expanding the media. ST strategy, one of the strategies is to open outlets or branches elsewhere to avoid long queues due to the narrow business location. The WO strategy, one of the strategies is to make unique packaging to attract consumers, to provide delivery facilities. WT strategy. there needs to be training for employees so that they can make quality instant products such as instant turmeric herbs, instant ginger, instant temulawak and other herbs. The best alternative strategy is to make instant ready-to-eat traditional medicines with different variants, provide delivery facilities, make unique and attractive packaging so that consumers are more interested in traditional medicinal products. Finally, providing training to employees to be able to make quality instant products.
Questions for Dictators: Question Times and Protests under Competitive Authoritarianism
Authors: Dwinger Felix
Published: August 2022

Question times under competitive authoritarianism offer opposition deputies a public arena for criticism. I argue that autocrats grant opposition elites regular opportunities to evaluate government performance as a credible commitment to discourage protests. In a model of Bayesian persuasion, I show that arbitrary threats of violence intimidate opposition legislators into softening their criticism. It creates a biased public signal which dissuades citizens from protesting in situations where their protests would have succeeded, had no question times occurred. This analysis clarifies the role of opposition deputies under competitive authoritarianism. Unlike previous research suggests, opposition elites should have no interest in informing service provision, although autocrats would prefer informational over biased question times. But autocrats can capitalize on incentives for opposition elites to manipulate beliefs about regime support. Moreover, covert repression is essential for information manipulation, a common survival strategy in modern autocracies, although previous research interprets these concepts as mutual replacements.
Language and the myth of scientific neutrality: a study of impersonality and clarity in academic researches’ elaboration manuals
Authors: Oliveira Paulo de Tarso , Vidal Maria Eunice Barbosa
Published: August 2022

The context that the present article is included is of the questions’ discussion about artificiality of the use of impersonal treatment in the language of scientific researches reports, especially in qualitative studies. Its fundamental question refers to what roles the manuals of elaboration of academic researchers can play – proposed by university institutions –regarding to communication in scientific reports. The highlighted aspects are of impersonality and passivity that are suggested in the academic manuals and its implications concerning clarity in qualitative research. Considering the actual context of dynamic tendencies in knowledge construction, this study is justified, mainly, because of the influence that the recommendations about writing scientific works, established by the university institutions, exert in the elaboration of research reports. Another aspect that is investigated refers to impersonal language that is perceived as a way to keep, in the reports, any influence exemption of the subject-researcher above the researched object. The aim is to describe and to understand the usage patterns of this manuals regarding to language characteristics’ recommendations that need to be followed when it comes to adequacy to various modalities of research. Under an outline of documental research, the data were collected from electronic websites of various university institutions that are localized in a delimited region that includes two Brazilian States. Other data that were collected include rules’ texts of Associação Brasileira de Normas Técnicas (ABNT) that refers to elaboration of academic works. The analysis, with interpretative bia, was done in two phases: in the beginning, one exploratory and, after one descriptive. The manual’s content was grouped in six analysis’ categories, according to the language characteristics. The results revealed that the manuals are not flexible about academic language and, they do not explain terms like clarity, objectivity and legimaticy of passive and active voices, compromising clear and updated writing that, in conclusion, could recognize the presence of the researcher as an information manager. So, the suggested inflexibility stes up as a serious obstacle to elaborate a compatible writing with the actual thought that refers to qualitative research.
Estimation of Some Epidemiological Parameters With the COVID-19 Data of Mayotte
Authors: Manou-Abi Solym M. , Slaoui Yousri , Balicchi Julien
Published: August 2022

We study in this article some statistical methods to fit some epidemiological parameters. We first consider a fit of the probability distribution which underlines the serial interval distribution of the COVID-19 on a given set of data collected on the viral shedding in patients with laboratory-confirmed. The best-fit model of the non negative serial interval distribution is given by a mixture of two Gamma distributions with different shapes and rates. Thus, we propose a modified version of the generation time function of the package R0. Second, we estimate the time-varying reproduction number in Mayotte. Using a justified mathematical learning model, we estimate the transmission parameters range values during the outbreak together with a sensitivity analysis. Finally, using some regression and forecasting methods, we give some learning models of the hospitalized, intensive care, and death cases over a given period. We end with a discussion and the limit of this study together with some forthcoming theoretical developments.
By characterizing metabolic and immune microenvironment reveal potential prognostic markers in the development of colorectal cancer
Authors: Liao Liangliang , Gao Yongjian , Su Jie , Feng Ye
Published: August 2022

Colon adenocarcinoma (COAD) is one of the deadliest cancers in the world and survival rates vary significantly between early and advanced stage patients. Therefore, the identification of the pathogenesis in the development of COAD and prognostic markers is urgently demanded. Herein, we collected RNA-seq and somatic mutation data of COAD for statistical analysis. Clinical stage-specific differentially expressed genes (DEGs) and tumor development-dependent DEGs were identified. By characterizing the metabolic and immune features of COAD between stages, we found that the energy supply and inflammatory response of advanced tumors were suppressed. Next, the ETS1, AR, GATA1, GATA2, SREBF1, FOXP3, STAT4, and NFKB1 were identified to drive the metabolic and immune-related pathways in the development of COAD. The three potential prognostic markers (HOXC8, IRF7, and CXCL13) were identified based on Cox regression analysis. Additionally, immune infiltration analysis revealed that the resting CD4+ T cell was significantly related to the overall survival (OS) of COAD patients. Collectively, the specific metabolic and immune characteristics of advanced patients and the identified prognostic biomarkers will contribute to the development of precision medicine.
Nanomedicines Targeting Metabolism in the Tumor Microenvironment
Authors: Ren Mengdi , Zheng Xiaoqiang , Gao Huan , Jiang Aimin , Yao Yu , He Wangxiao
Published: August 2022

Cancer cells reprogram their metabolism to meet their growing demand for bioenergy and biosynthesis. The metabolic profile of cancer cells usually includes dysregulation of main nutritional metabolic pathways and the production of metabolites, which leads to a tumor microenvironment (TME) having the characteristics of acidity, hypoxic, and/or nutrient depletion. Therapies targeting metabolism have become an active and revolutionary research topic for anti-cancer drug development. The differential metabolic vulnerabilities between tumor cells and other cells within TME provide nanotechnology a therapeutic window of anti-cancer. In this review, we present the metabolic characteristics of intrinsic cancer cells and TME and summarize representative strategies of nanoparticles in metabolism-regulating anti-cancer therapy. Then, we put forward the challenges and opportunities of using nanoparticles in this emerging field.
Tendon mechanical properties are enhanced via recombinant lysyl oxidase treatment
Authors: Nguyen Phong K. , Jana Aniket , Huang Chi , Grafton Alison , Holt Iverson , Giacomelli Michael , Kuo Catherine K.
Published: August 2022

Tendon mechanical properties are significantly compromised in adult tendon injuries, tendon-related birth defects, and connective tissue disorders. Unfortunately, there currently is no effective treatment to restore native tendon mechanical properties after postnatal tendon injury or abnormal fetal development. Approaches to promote crosslinking of extracellular matrix components in tendon have been proposed to enhance insufficient mechanical properties of fibrotic tendon after healing. However, these crosslinking agents, which are not naturally present in the body, are associated with toxicity and significant reductions in metabolic activity at concentrations that enhance tendon mechanical properties. In contrast, we propose that an effective method to restore tendon mechanical properties would be to promote lysyl oxidase (LOX)-mediated collagen crosslinking in tendon during adult tissue healing or fetal tissue development. LOX is naturally occurring in the body, and we previously demonstrated LOX-mediated collagen crosslinking to be a critical regulator of tendon mechanical properties during new tissue formation. In this study, we examined the effects of recombinant LOX treatment on tendon at different stages of development. We found that recombinant LOX treatment significantly enhanced tensile and nanoscale tendon mechanical properties without affecting cell viability or collagen content, density, and maturity. Interestingly, both tendon elastic modulus and LOX-mediated collagen crosslink density plateaued at higher recombinant LOX concentrations, which may have been due to limited availability of adjacent lysine residues that are near enough to be crosslinked together. The plateau in crosslink density at higher concentrations of recombinant LOX treatments may have implications for preventing over-stiffening of tendon, though this requires further investigation. These findings demonstrate the exciting potential for a LOX-based therapeutic to enhance tendon mechanical properties via a naturally occurring crosslinking mechanism, which could have tremendous implications for an estimated 32 million acute and chronic tendon and ligament injuries each year in the U.S.
Strategies to improve the therapeutic effect of pluripotent stem cell-derived cardiomyocytes on myocardial infarction
Authors: Xiao Yang , Chen Yihuan , Shao Chunlai , Wang Yaning , Hu Shijun , Lei Wei
Published: August 2022

Myocardial infarction (MI) is a common cardiovascular disease caused by permanent loss of cardiomyocytes and the formation of scar tissue due to myocardial ischemia. Mammalian cardiomyocytes lose their ability to proliferate almost completely in adulthood and are unable to repair the damage caused by MI. Therefore, transplantation of exogenous cells into the injured area for treatment becomes a promising strategy. Pluripotent stem cells (PSCs) have the ability to proliferate and differentiate into various cellular populations indefinitely, and pluripotent stem cell-derived cardiomyocytes (PSC-CMs) transplanted into areas of injury can compensate for part of the injuries and are considered to be one of the most promising sources for cell replacement therapy. However, the low transplantation rate and survival rate of currently transplanted PSC-CMs limit their ability to treat MI. This article focuses on the strategies of current research for improving the therapeutic efficacy of PSC-CMs, aiming to provide some inspiration and ideas for subsequent researchers to further enhance the transplantation rate and survival rate of PSC-CMs and ultimately improve cardiac function.
Ship wake forcing and performance of a living shoreline segment on an estuarine shoreline
Authors: Everett Cassandra L. , Williams Oscar , Ruggiero Emma , Larner Michael , Schaefer Rachel , Malej Matt , Shi Fengyan , Bruck Jules , Puleo Jack A.
Published: August 2022

Investigation of the effectiveness of Natural and Nature-Based Features (NNBF) for protecting shorelines from ship wake is increasingly important with continued development along the coast, especially when combined with sea level rise. Studies that investigate the wave energy dissipation capacity of different techniques and account for variation in context will lead to improvements and innovation in designed NNBF. Few studies have examined the performance of NNBF in protecting shorelines from ship wake. In this study of a low-sloping estuarine shoreline adjacent to a major shipping route, a natural design using coir logs and wooden staking was implemented in a T-head groin configuration. Pressure transducers and electromagnetic current meters were deployed over ∼1.5 months to investigate the energy dissipation and velocity attenuation capabilities of the installation. Results indicate that ship wakes account for 25%–50% of the total daily energy impacting the shoreline at the study site. Peak background velocities are typically over 50% smaller than the largest ship wake velocities. Field data and results of the fully nonlinear Boussinesq model, FUNWAVE-TVD, indicate that the installation is capable of decreasing energy impacting the shoreline by 10%–80% and is effective over the lower 50% of the tidal range and when submerged up to twice its height. Elevation surveys of the site indicate accrual of sediment within the installation, suggesting wave diffraction patterns promoting further accretion at the site over time. Observations indicate that coir logs may be effective in reducing wave energy from ship wakes but may fail under storm conditions in a moderate fetch confined channel. Findings from this study illustrate the opportunities and challenges nature-based solutions face in addressing ship wakes, and their ability to protect shorelines under high energy stressors.
Integrated network pharmacology and hepatic metabolomics to reveal the mechanism of Acanthopanax senticosus against major depressive disorder
Authors: Gu Xinyi , Zhang Guanying , Wang Qixue , Song Jing , Li Ying , Xia Chenyi , Zhang Ting , Yang Li , Sun Jijia , Zhou Mingmei
Published: August 2022

Objective:Acanthopanax senticosus(Rupr. et Maxim.) Harms (ASH) is a traditional herbal medicine widely known for its antifatigue and antistress effects, as well as tonifying qi, invigorating spleen and kidney, and tranquilizing the mind. Recent evidence suggests that ASH has a therapeutic effect on major depressive disorder (MDD), but its mechanism is still unclear. The current study aimed to investigate the effect of ASH on MDD and potential therapeutic mechanisms.

Materials and Methods:The chemical compound potential target network was predicted based on network pharmacology. Simultaneously, chronic unpredictable mild stress (CUMS) model mice were orally administrated ASH with three dosages (400, 200, and 100 mg/kg) for 6 weeks, and hepatic metabolomics based on gas chromatography–mass spectrometry (GC–MS) was carried out to identify differential metabolites and related metabolic pathways. Next, the integrated analysis of metabolomics and network pharmacology was applied to find the key target. Finally, molecular docking technology was employed to define the combination of the key target and the corresponding compounds.

Results:A total of 13 metabolites and four related metabolic pathways were found in metabolomics analysis. From the combined analysis of network pharmacology and metabolomics, six targets (DAO, MAOA, MAOB, GAA, HK1, and PYGM) are the overlapping targets and two metabolic pathways (glycine, serine, and threonine metabolism and starch and sucrose metabolism) are the most related pathways. Finally, DAO, MAOA, MAOB, GAA, HK1, and PYGM were verified bounding well to their corresponding compounds including isofraxidin, eleutheroside B1, eleutheroside C, quercetin, kaempferol, and acacetin.

Conclusion:Based on these results, it was implied that the potential mechanism of ASH on MDD was related to the regulation of metabolism of several excitatory amino acids and carbohydrates, as well as the expression of DAO, MAOA, MAOB, GAA, HK1, and PYGM.
Regulatory role of RNA N6-methyladenosine modifications during skeletal muscle development
Authors: Yu Baojun , Liu Jiamin , Zhang Juan , Mu Tong , Feng Xiaofang , Ma Ruoshuang , Gu Yaling
Published: August 2022

Functional cells in embryonic myogenesis and postnatal muscle development undergo multiple stages of proliferation and differentiation, which are strict procedural regulation processes. N6-methyladenosine (m6A) is the most abundant RNA modification that regulates gene expression in specific cell types in eukaryotes and regulates various biological activities, such as RNA processing and metabolism. Recent studies have shown that m6A modification-mediated transcriptional and post-transcriptional regulation plays an essential role in myogenesis. This review outlines embryonic and postnatal myogenic differentiation and summarizes the important roles played by functional cells in each developmental period. Furthermore, the key roles of m6A modifications and their regulators in myogenesis were highlighted, and the synergistic regulation of m6A modifications with myogenic transcription factors was emphasized to characterize the cascade of transcriptional and post-transcriptional regulation during myogenesis. This review also discusses the crosstalk between m6A modifications and non-coding RNAs, proposing a novel mechanism for post-transcriptional regulation during skeletal muscle development. In summary, the transcriptional and post-transcriptional regulatory mechanisms mediated by m6A and their regulators may help develop new strategies to maintain muscle homeostasis, which are expected to become targets for animal muscle-specific trait breeding and treatment of muscle metabolic diseases.
Neospora caninum Infection Triggers S-phase Arrest and Alters Nuclear Characteristics in Primary Bovine Endothelial Host Cells
Authors: Velásquez Zahady D. , Rojas-Barón Lisbeth , Larrazabal Camilo , Salierno Marcelo , Gärtner Ulrich , Pervizaj-Oruqaj Learta , Herold Susanne , Hermosilla Carlos , Taubert Anja
Published: August 2022

Neospora caninumrepresents a major cause of abortive disease in bovines and small ruminants worldwide. As a typical obligate intracellular apicomplexan parasite,N. caninumneeds to modulate its host cell for successful replication. In the current study, we focused on parasite-driven interference with host cell cycle progression. By performing DNA content-based cell cycle phase analyses inN. caninum-infected primary bovine umbilical vein endothelial cells (BUVEC), a parasite-driven S-phase arrest was detected at both 24 and 32 h p. i., being paralleled by fewer host cells experiencing the G0/G1 cell cycle phase. When analyzing S-subphases, proliferation cell nuclear antigen (per PCNA)-based experiments showed a reduced population of BUVEC in the late S-phase. Analyses on key molecules of cell cycle regulation documented a significant alteration of cyclin A2 and cyclin B1 abundance inN. caninum-infected host endothelial cells, thereby confirming irregularities in the S-phase and S-to-G2/M-phase transition. In line with cell cycle alterations, general nuclear parameters revealed smaller nuclear sizes and morphological abnormalities of BUVEC nuclei within theN. caninum-infected host cell layer. The latter observations were also confirmed by transmission electron microscopy (TEM) and by analyses of lamin B1 as a marker of nuclear lamina, which illustrated an inhomogeneous nuclear lamin B1 distribution, nuclear foldings, and invaginations, thereby reflecting nuclear misshaping. Interestingly, the latter finding applied to both non-infected and infected host cells within parasitized BUVEC layer. Additionally, actin detection indicated alterations in the perinuclear actin cap formation since typical nucleo-transversal filaments were consistently lacking inN. caninum-infected BUVEC, as also documented by significantly decreased actin-related intensities in the perinuclear region. These data indicate thatN. caninumindeed alters host cell cycle progression and severely affects the host cell nuclear phenotype in primary bovine endothelial host cells. In summary, these findings add novel data on the complexN. caninum-specific modulation of host cell and nucleus, thereby demonstrating clear differences in cell cycle progression modulation driven by other closely related apicomplexans likeToxoplasma gondiiandBesnotia besnoiti.
Phase transformation mechanism of MnCO3 as cathode materials for aqueous zinc-ion batteries
Authors: Zheng Junjie , Liu Pengcheng , Yao Jia , Gan Yi , Li Jingying , Wang Cong , Liu Xiang , Rao Yiheng , Ma Guokun , Lv Lin , Wang Hanbin , Tao Li , Zhang Jun , Wang Hao
Published: August 2022

Aqueous rechargeable zinc-ion batteries (ZIBs) have been given more and more attention because of their high specific capacity, high safety, and low cost. The reasonable design of Mn-based cathode materials is an effective way to improve the performance of ZIBs. Herein, a square block MnCO3 electrode material is synthesized on the surface of carbon cloth by a one-step hydrothermal method. The phase transition of MnCO3 was accompanied by the continuous increase of specific capacity, and finally maintained good cycle stability in the charge–discharge process. The maximum specific capacity of MnCO3 electrode material can reach 83.62 mAh g−1 at 1 A g−1. The retention rate of the capacity can reach 85.24% after 1,500 cycles compared with the stable capacity (the capacity is 61.44 mAh g−1 under the 270th cycle). Ex situ characterization indicates that the initial MnCO3 gradually transformed into MnO2 accompanied by the embedding and stripping of H+ and Zn2+ in charge and discharge. When MnCO3 is no longer transformed into MnO2, the cycle tends to be stable. The phase transformation of MnCO3 could provide a new research idea for improving the performance of electrode materials for energy devices.
Sensitive electrochemical detection of p-nitrophenol by pre-activated glassy carbon electrode integrated with silica nanochannel array film
Authors: Su Ruobing , Tang Hongliang , Xi Fengna
Published: August 2022

Convenient, rapid and sensitive detection of p-nitrophenol (p-NP), one of the priority environmental pollutants, in environmental samples is of great significance. Electrochemical sensor with simple fabrication process, high sensitivity and selectivity, good antifouling, and regeneration performance is highly desirable. Herein, an electrochemical sensing platform is demonstrated based on the integration of vertically-ordered mesoporous silica-nanochannel film (VMSF) on electrochemical pre-activated glassy carbon electrode (p-GCE), which is able to realize ultrasensitive detection of p-NP in environmental samples. Electrochemical pre-activation of GCE is achieved through a simple and green electrochemical polarization process including anodic oxidation at high voltage and the following cathodic reduction at low voltage. The p-GCE possesses enhanced active area and introduced active sites, and enables stable binding of VMSF. VMSF is easily grown on p-GCE through the electrochemically assisted self-assembly (EASA) method within 10 s. Owing to the hydrogen bonding between silanol groups and p-NP, VMSF nanochannels display strong enrichment effect for the detection of p-NP. The developed VMSF/p-GCE sensor can achieve sensitive detection of p-NP ranging from 10 nM to 1 μM and from 1 to 30 μM with a limit of detection (LOD) of 9.4 nM. Considering the antifouling ability of VMSF, detection of p-NP in pond water is achieved.
Unexpected kinetically controlled organoselenium-based isomaleimide: X-ray structure, hirshfeld surface analysis, 3D energy framework approach, and density functional theory calculation
Authors: Shaaban Saad , Ferjani Hela , Abd El-Lateef Hany M. , Khalaf Mai M. , Gouda Mohamed , Alaasar Mohamed , Yousef Tarek A.
Published: August 2022

Reduction of 4,4′-diselanediyldianiline (1) followed by the reaction with bromo-4-(bromomethyl)benzene afforded the corresponding 4-((4-bromobenzyl)selanyl)aniline (2) in 85% yield. N-Maleanilic acid 3 was obtained in 94% yield via the reaction of selenoamine 2 with toxilic anhydride. Subsequent dehydration of N-maleanilic acid 3 using acetic anhydride furnished the unexpected isomaleimide 5-((4-((4-bromophenyl)selanyl)phenyl)imino)furan-2(5H)-one (4) instead of the maleimide 5. The molecular structure of compound 4 was confirmed by mass spectrometry, 1H- and 13C-NMR spectroscopy, and X-ray diffraction analysis. Their cytotoxicity was assessed against two oligodendrocytes, and their respective redox properties were evaluated using 2′,7′-dichlorodihydrofluorescein diacetate (H2-DCFDA) assay. Furthermore, their antiapoptotic potential was also evaluated by flow cytometry. The compound crystallizes in triclinic P-1 space group with unit cell parameters a = 5.7880 (4) Å, b = 9.8913 (6) Å, c = 14.5951 (9) Å, V = 1731.0 (3) Å3 and Z = 2. The crystal packing is stabilized by intermolecular hydrogen bonding, π···π, C-Br···π stacking interactions, and other non-covalent interactions. The mapping of different Hirshfeld surfaces and 2D-fingerprint were used to investigate intermolecular interactions. The interaction energies that stabilize the crystal packing were calculated and graphically represented as framework energy diagrams. We present a computational investigation of compound 4’s molecular structure at the Density Functional Theory level using the B3LYP method and the 6-31G ++ basis set in this paper. The optimized structure matches the experimental outcome. The global reactivity descriptors and molecular electrostatic potential (M.E.P.) map emphasize the molecule’s reactive locations, allowing reactivity prediction. The charge transfer properties of molecules can be estimated by examining Frontier molecular orbitals.