bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainability in Peripheral and Ultra-Peripheral Rural Areas through a Multi-Attribute Analysis: The Case of the Italian Insular Region
Authors: Zolin M. Bruna , Ferretti Paola , Grandi Mirco
Published: November 2020

Italy has adopted the strategy of inner areas, mainly based on physical distance from public services. The strategy promotes a multi-level and multi-fund governance approach and the local partnership of mayors. Our paper focuses on rural areas, identified by the national strategy of inner areas, as peripheral and ultra-peripheral, in the Italian insular region (Sicily and Sardinia). It analyzes, at the municipality level, socio-demographic, economic, and environmental sustainability using appropriate indicators. Aiming at discovering the underlying relationship portrayed by multi-attribute data in an information system, we applied rough set theory. The inductive decision rules obtained through this data mining methodology reveal the simultaneous presence or absence of important characteristics aiming at reaching different levels of sustainability. Without the requirement of statistical assumptions regarding data distribution or structures for collecting data, such as functions or equations, this method ensures the description of patterns exhibited by data. Of particular interest is the assessment of conditional attributes (i.e., the selected indicators), and the information connecting them to sustainability, as a decision attribute. The most important result is rule generation, specifically, decision rules that are able to suggest tools for policy makers at different levels.
Carbon and Nitrogen Stocks and Soil Quality in an Area Cultivated with Guinea Grass under the Residual Effect of Nitrogen Doses
Authors: Gurgel Antonio Leandro Chaves , Difante Gelson dos Santos , Araujo Alexandre Romeiro de , Montagner Denise Baptaglin , Euclides Valeria Pacheco Batista , da Silva Manoel Gustavo Paranhos
Published: November 2020

This study examines the residual effect of nitrogen (N) doses on the carbon (C) and N stocks and on soil quality in an area cultivated with guinea grass. The pastures received three annual doses of N (100, 200 and 300 kg ha−1) from 2015 to 2017. In 2018, N fertilization was not applied so the residual effect of the nutrient could be characterized. Soil chemical attributes, C and N stocks, density and penetration resistance and root system characteristics were evaluated at different depths. No difference was observed between the N doses for soil density, which averaged 1.22 and 1.25 g cm−3 under and between the tussocks of guinea grass, respectively. Penetration resistance was affected by the N doses post-grazing, with the highest value (1.9 MPa) observed in pastures that received 300 kg ha−1 of N for three consecutive years. Root dry mass was not affected by the N doses. There was no effect of N doses on the average (19.7 mg ha−1) or total (134.3 mg ha−1) C stock in the soil. The total N stock did not change (11.3 mg ha−1) in response to the N doses; however, the average N stock was higher in the soil cultivated with guinea grass under the N dose of 300 kg ha−1 (1.7 mg ha−1). The N doses had little interference with the soil chemical and physical aspects. Regardless of the dose, high C and N stocks were observed in the soil cultivated with guinea grass. Therefore, when properly managed, intensive pasture-based animal production systems become important allies of the environment.
The Urban Museum as a Creative Tourism Attraction: London Museum Lates Visitor Motivation
Authors: Choi Ayeon , Berridge Graham , Kim Chulwon
Published: November 2020

The urban museum has become a multi-functional institution that transcends the functional display of artifacts. The museum has become, in cities, a hybrid institution that keeps its doors open late to support a wide variety of special events. This study explored London’s “Museum Lates” programs and event characteristics, theoretical views of museums as cultural and creative tourism attractions. Furthermore, the study applied the contextual model of learning to understand visitor attendance motivations. The authors employed an interpretive approach using interviews with two types of participants: event visitors and event coordinators. The findings suggest that attendees possess personal, physical, and socio-cultural motivations for participating in “Museum Lates” events. The characteristics of late events—extraordinary quality and evening-time atmosphere—produce different effects from those of current museum exhibitions held during regular operating hours. “Museum Lates” events can contribute to achieving cultural sustainability, adding a cultural construct to the traditional three pillars of sustainability.
Social Networks as Drivers for Technology Adoption: A Study from a Rural Mountain Area in Italy
Authors: Filippini Rosalia , Marescotti Maria Elena , Demartini Eugenio , Gaviglio Anna
Published: November 2020

Innovation processes includes social and communicative elements. The role of innovative technology for the development of farming systems is investigated in literature, but only a few studies deal with the influence of networks on the adoption of technologies by farmers. The aim of this paper is to verify if the adoption of smartphones for professional reasons by farmers is influenced by the networks to which farmers belong, the socio-demographic characteristics of the farmers, and their farm’s size. The case study is in the Valtellina valley, a rural mountain area in the north of Italy, where 53 livestock farmers were interviewed. The methodology is based on two steps. First, social network analysis is applied to diagnose the relationships of farmers in terms of connectivity and closeness and to detect the centrality measures of farmers in three different social relationships: production networks, market networks, and information exchange networks. A multiple linear regression model is then applied to test whether centrality measures of the three networks, the farmers’ socio-demographic characters, and the farm’s features drive the adoption of smartphones for professional reasons. Results suggest that the centrality measures in production networks positively drive the adoption of smartphones, while the centrality measures of market and information exchange networks do not have the same effect. At the same time, the farmers’ age negatively affects the use of smartphones for professional reasons, while the size of the herd, and the education and gender of farmers have no significant influence. The study contributes to the debate around the Agricultural Knowledge Innovation System and supports local policies based on the inclusion of farmers in the technological development in rural areas.
Spatiotemporal Differentiation of Soil Organic Carbon of Grassland and Its Relationship with Soil Physicochemical Properties on the Northern Slope of Qilian Mountains, China
Authors: Yang Le , Jia Wenxiong , Shi Yang , Zhang Zhiyuan , Xiong Hui , Zhu Guofeng
Published: November 2020

The soil organic carbon pool is an important part of the global carbon cycle, and its accumulation and decomposition affect the balance of the global carbon cycle. It is important to understand scientifically the temporal and spatial variation of soil organic carbon (SOC) and its influencing factors, which could aid further understanding of the accumulation and decomposition of SOC. In order to reveal the relationship between soil organic carbon and soil’s physicochemical properties, six plots were selected on the east, middle and west of forest steppes and typical grasslands on the northern slope of Qilian Mountains during two consecutive growing seasons from 2013 to 2014. Soil samples under 0–30 cm were used to study the spatiotemporal differentiation of SOC and its relationship with the soil’s physicochemical properties in the grassland of the study area. The results show that the content of SOC in the grassland in 2013 was higher than that in 2014, and that it decreased gradually from east to west. The content of SOC is significantly different between the soil layer of 0–10 cm and the soil layers of 10–20 cm and 20–30 cm (p < 0.05), and it decreases with increases in soil depth. The SOC content on forest steppe is higher than that on typical grassland. Significant positive correlations appear between SOC with soil water content and soil nutrients (alkaline nitrogen, available phosphorus, available potassium) (p < 0.01), but there are significant negative correlations between SOC and soil temperature, soil pH, and soil electrical conductivity (p < 0.01).
Atmospheric Carbon Dioxide and Electricity Production Due to Lockdown
Authors: Yusup Yusri , Ramli Nur Kamila , Kayode John Stephen , Yin Chee Su , Hisham Sabiq , Mohamad Isa Hassim , Ahmad Mardiana Idayu
Published: November 2020

We analyzed real-time measurements of atmospheric carbon dioxide (CO2), with total electricity production and nationwide restrictions phases in China, the United States of America, Europe, and India due to the novel coronavirus COVID-19 pandemic and its effects on atmospheric CO2. A decline of 3.7% in the global energy demand at about 150 million tonnes of oil equivalent (Mtoe) in the first quarter (Q1) of 2020 was recorded compared to Q1 2019 due to the cutback on international economic activities. Our results showed that: (1) electricity production for the same period in 2018, 2019, and 2020 shrunk at an offset of 9.20%, which resulted in a modest reduction (−1.79%) of atmospheric CO2 to the 2017–2018 CO2 level; (2) a non-seasonal, abrupt, and brief atmospheric CO2 decrease by 0.85% in mid-February 2020 could be due to Phase 1 restrictions in China. The results indicate that electricity production reduction is significant to the short-term variability of atmospheric CO2. It also highlights China’s significant contribution to atmospheric CO2, which suggests that, without the national restriction of activities, CO2 concentration is set to exceed 2019 by 1.79%. Due to the lockdown, it quickly decreased and sustained for two months. The results underscore atmospheric CO2 reductions on the monthly time scale that can be achieved if electricity production from combustible sources was slashed. The result could be useful for cost-benefit analyses on the decrease in electricity production of combustible sources and the impact of this reduction on atmospheric CO2.
The Unknown Potential of Blockchain for Sustainable Supply Chains
Authors: Varriale Vincenzo , Cammarano Antonello , Michelino Francesca , Caputo Mauro
Published: November 2020

The growing attention to emerging technologies has raised several doubts regarding the sustainability aspects. Among the new technologies, blockchain has laid the foundations for a revolution in the financial field but also in the energy, tourism, medical, industrial and supply chains sectors. Through a systematic literature review, 37 documents were analyzed to describe the sustainable aspects generated using blockchain in supply chains. Therefore, the results achieved provide two levels of analysis. The former highlights the impact of the adoption of blockchain in supply chains based on the three dimensions of sustainability: environmental, economic and social. The latter shows the positive and negative impacts of each form of sustainability. The purpose of the work is to summarize the current state of the art in order to propose a future development agenda based on the need to simulate and define a measurability for each dimension of sustainability. Finally, the study provides a broad overview of practical and managerial implications for entrepreneurs and researchers. On the one hand, the work shows how the use of blockchain improves the profits and reputation of companies. On the other hand, it highlights several research gaps to be investigated and considered by researchers.
Gaining Insight into Violence from Gender Stereotypes and Sexist Attitudes in the Context of Tourism
Authors: Díaz-Meneses Gonzalo , Vilkaitė-Vaitonė Neringa , Estupiñan-Ojeda Miriam
Published: November 2020

It is difficult to identify, but there is a type of harassment grounded in gender stereotyping in the context of tourism. It would be useful to discover the hidden relationships between gender harassment and certain beliefs about women as travellers, tourism professionals and sex objects in the field of hospitality. Methodologically, a survey was carried out reaching a sample of ±684 units by means of a convenience sampling procedure. The measuring instruments consisted of a structured questionnaire divided into two kinds of Likert beliefs scales comprising general statements and statements related to tourism. The survey respondent data were also gathered as regards sociodemographic characteristics. This paper presents empirical evidence to identify the causal factors of gender violence by considering general and particular stereotyping in tourism. Firstly, by performing three exploratory factor analyses, three female stereotyping dimensions were labelled in the field of tourism (occupational sexism, ambivalent sexist discrimination, and sex as a commodity), as well as three general prejudices about women (dysfunctional romantic relationships, ethically challenged presumptions, and aesthetical manners conventions) and the gender harassment factor. Secondly, a linear regression analysis was carried out to demonstrate that both general stereotyping related to a broader “life” framework and sectorial prejudices in tourism cause gender harassment. Finally, this research proves that general stereotyping determines sectorial prejudices in the field of tourism. The practical implications could be to enhance gender equality and combat gender harassment by revealing unintentional and unobserved prejudices that occur in a general life setting and in the tourism sphere against women as neglected professionals, under the subtle and ambivalent condition of travellers, and even as objects of consumption.
Assessing the Legitimacy of Technological Innovation in the Public Sphere: Recovering Raw Materials from Waste Water
Authors: Blankesteijn Marlous , Bossink Bart
Published: November 2020

This paper researches legitimacy creation in a publicly-funded trajectory of innovative technological development. It develops a framework of input, throughput and output legitimacy. The framework is developed based on a review of the literature on the creation of legitimacy in innovative technological development. The framework assists in further exploring the potential of the integrated assessment of the legitimacy of technological innovation trajectories in the public sphere, in terms of (1) public accountability (ensuring input legitimacy); (2) science, technology and innovation policy (ensuring throughput legitimacy); and (3) the potential for the implementation of the technology itself in practical contexts (ensuring output legitimacy). The framework is used to analyze a case study about the publicly-funded development of innovative technology for the retrieval of raw materials from waste water. Theoretically, the value of a more processual approach to the conceptualization of legitimacy becomes apparent. Furthermore, the framework assists in the development of practical recommendations on the ways in which to optimize the legitimacy in an earlier stage in the innovation’s trajectory. However, due attention should also be paid to the role of regulatory arrangements in the optimization of the legitimacy of publicly-funded technological innovation. This is an avenue for further research.
Service Quality and Service Gap of Autonomous Driving Group Rapid Transit System
Authors: Hung Wei-Hsi , Hsu Yao-Tang
Published: November 2020

Recently, the trend of public transportation has evolved from traditional vehicles to intelligent transportation systems. Among many innovative systems, the development of group rapid transit (GRT) has become increasingly important. This study aims to explore the key acceptance factors for users to adopt GRT through three dimensions: technology, sharing, and experiential marketing (TSE). First, this study identifies variables under each construct of the TSE model through a literature review and interviews with experts, so as to understand what factors of the model impact users’ usage intention and continuous usage intention. Subsequently, through a questionnaire survey, the theoretical model is verified. The participants of the survey were users of GRT, and a total of 306 valid questionnaires were collected. Through structural equation modeling (SEM) analysis, the results indicate that technology does not significantly impact usage intention, as users may not fully understand GRT’s future developments; technology only affects continuous usage intention. Sharing also only influences continuous usage intention. These results show that the adoption of GRT may be gradual and long-term rather than short-term. Finally, experiential marketing has a significant impact on both usage intention and continuous usage intention. This implies that users’ experiences are vital in promoting innovative services, hence service providers should seek to not only improve the service but also enhance users’ trust in and support for the service.
Cell-Based Meat and Firms’ Environmental Strategies: New Rationales as per Available Literature
Authors: Reis Germano Glufke , Heidemann Marina Sucha , Matos Katherine Helena Oliveira de , Molento Carla Forte Maiolino
Published: November 2020

Higher demand for meat production and limited inputs, as well as environmental and animal ethics issues, are bringing alternative protein sources to the market, such as cell-based meat (CBM), i.e., meat produced through cell culturing, without involving animal raising and killing. Although the potential social and environmental benefits of the technology have been recently addressed in the blossoming CBM literature, little has been discussed about the possible implications for the environmental strategies of firms that are entering the new cell-based production chain. Thus, drawing on the theoretical framework of competitive environmental strategies and a systematic review of the literature, we discuss prospects for cell-based meat regarding the possible adoption of environmental strategies by firms that are entering the CBM chain. The technology may be considered a potential means for mitigating most of the environmental impacts of large-scale meat production, e.g., extensive land use and greenhouse gas emissions. We discuss how such benefits and consumer attitudes towards cultivated meat could encourage the adoption of environmental strategies by firms, and the roles that value chain firms are likely to play in those strategies in the future.
Social Sustainability in Adolescents’ Music Event Attendance
Authors: Kinnunen Maarit , Homi Harri , Honkanen Antti
Published: November 2020

This study focuses on social sustainability of music events in adolescents’ lives through their perceptions and own words as they describe their live music experiences. Scrutinizing music event attendance from the social sustainability perspective demonstrates that the cultural content per se is not as meaningful as the social network that comes together in such events. The research questions were: How is social sustainability manifested in adolescents’ music event attendance, and what are the implications? The research data comprised the results of a web survey directed at 15–18 year old adolescents. Bonding and bridging, as well as the sense of community, were present in adolescents’ descriptions of live music experiences, producing various forms of well-being effects. The sense of belonging was almost missing from the narratives, which suggests that how adolescents consume music has a decaying interest in the grassroots culture that fosters the sense of belonging. This has major implications for the development of popular culture.
Regulatory Compliance of Community-Based Conservation Organizations: Empirical Evidence from Annapurna Conservation Area, Nepal
Authors: Baral Nabin , Heinen Joel T.
Published: November 2020

Community-based conservation in the developing world generally puts more emphasis on voluntary commitments and compliance rather than enforcement of formal laws and regulations for the governance of protected areas. However, as with other forms of organizational management, once institutions are established, they are required to comply with all relevant, legally binding regulations. Furthermore, it is broadly assumed that compliance with established regulations is critical for good governance. In this paper, we review these matters through an empirical study of Conservation Area Management Committees’ degree of compliance with regulations under Nepalese law, within the Annapurna Conservation Area—one of the best-known community-based protected areas worldwide—based on quantitative content analysis of the committees’ meeting minutes from 2008 to 2012. According to the established rules, two to four women and one to five minorities serve as committee members in each instance. On average, fewer members than expected attended meetings, and the number of decisions made per meeting showed a curvilinear relationship with the number of members present as well as their demographic diversity. Of the 13 committees selected for study, only two met the legal mandate of holding six regular meetings annually within two-month intervals. In all the other cases, non-compliance was noted for one to all five years of the committees’ terms. In general, compliance declined over the five-year terms, and some committees were significantly less-compliant than others. Although enforceable decisions were made within both compliant and non-compliant committees, several problems of non-compliance were identified that may affect conservation outcomes. We suggest several possible reasons for non-compliance and argue that these may be symptoms of institutional weaknesses. Organizations that fail to meet their commitments risk liability and may also lose the formal legal authority to govern. Regular monitoring is recommended to address compliance issues.
Dynamic Symmetry in Dozy-Chaos Mechanics
Authors: Egorov Vladimir V.
Published: November 2020

All kinds of dynamic symmetries in dozy-chaos (quantum-classical) mechanics (Egorov, V.V. Challenges 2020, 11, 16; Egorov, V.V. Heliyon Physics 2019, 5, e02579), which takes into account the chaotic dynamics of the joint electron-nuclear motion in the transient state of molecular “quantum” transitions, are discussed. The reason for the emergence of chaotic dynamics is associated with a certain new property of electrons, consisting in the provocation of chaos (dozy chaos) in a transient state, which appears in them as a result of the binding of atoms by electrons into molecules and condensed matter and which provides the possibility of reorganizing a very heavy nuclear subsystem as a result of transitions of light electrons. Formally, dozy chaos is introduced into the theory of molecular “quantum” transitions to eliminate the significant singularity in the transition rates, which is present in the theory when it goes beyond the Born–Oppenheimer adiabatic approximation and the Franck–Condon principle. Dozy chaos is introduced by replacing the infinitesimal imaginary addition in the energy denominator of the full Green’s function of the electron-nuclear system with a finite value, which is called the dozy-chaos energy γ. The result for the transition-rate constant does not change when the sign of γ is changed. Other dynamic symmetries appearing in theory are associated with the emergence of dynamic organization in electronic-vibrational transitions, in particular with the emergence of an electron-nuclear-reorganization resonance (the so-called Egorov resonance) and its antisymmetric (chaotic) “twin”, with direct and reverse transitions, as well as with different values of the electron–phonon interaction in the initial and final states of the system. All these dynamic symmetries are investigated using the simplest example of quantum-classical mechanics, namely, the example of quantum-classical mechanics of elementary electron-charge transfers in condensed media.
A Distinctive Symmetric Analyzation of Improving Air Quality Using Multi-Criteria Decision Making Method under Uncertainty Conditions
Authors: Narayanamoorthy Samayan , Anuja Arumugam , Kang Daekook , Kureethara Joseph Varghese , Kalaiselvan Samayan , Manirathinam Thangaraj
Published: November 2020

This world has a wide range of technologies and possibilities that are available to control air pollution. Still, finding the best solution to control the contamination of the air without having any impact on humans is a complicated task. This proposal helps to improve the air quality using the multi-criteria decision making method. The decision to improve air quality is a challenging problem with today’s technology and environmental development level. The multi-criteria decision making method is quite often faced with conditions of uncertainty, which can be tackled by employing fuzzy set theory. In this paper, based on an objective weighting method (CCSD), we explore the improved fuzzy MULTIMOORA approach. We use the classical Interval-Valued Triangular Fuzzy Numbers (IVTFNs), viz. the symmetric lower and upper triangular numbers, as the basis. The triangular fuzzy number is identified by the triplets; the lowest, the most promising, and the highest possible values, symmetric with respect to the most promising value. When the lower and upper membership functions are equated to one, we get the normalized interval-valued triangular fuzzy numbers, which consist of symmetric intervals. We evaluate five alternatives among the four criteria using an improved MULTIMOORA method and select the best method for improving air quality in Tamil Nadu, India. Finally, a numerical example is illustrated to show the efficiency of the proposed method.
Comparison between the Thomas–Fermi and Hartree–Fock–Bogoliubov Methods in the Inner Crust of a Neutron Star: The Role of Pairing Correlations
Authors: Shelley Matthew , Pastore Alessandro
Published: November 2020

We investigated the role of a pairing correlation in the chemical composition of the inner crust of a neutron star with the extended Thomas–Fermi method, using the Strutinsky integral correction. We compare our results with the fully self-consistent Hartree–Fock–Bogoliubov approach, showing that the resulting discrepancy, apart from the very low density region, is compatible with the typical accuracy we can achieve with standard mean-field methods.
Put Some Guts into It: Intestinal Organoid Models to Study Viral Infection
Authors: García-Rodríguez Inés , Sridhar Adithya , Pajkrt Dasja , Wolthers Katja C.
Published: November 2020

The knowledge about enteric viral infection has vastly increased over the last eight years due to the development of intestinal organoids and enteroids that suppose a step forward from conventional studies using cell lines. Intestinal organoids and enteroids are three-dimensional (3D) models that closely mimic intestinal cellular heterogeneity and organization. The barrier function within these models has been adapted to facilitate viral studies. In this review, several adaptations (such as organoid-derived two-dimensional (2D) monolayers) and original intestinal 3D models are discussed. The specific advantages and applications, as well as improvements of each model are analyzed and an insight into the possible path for the field is given.
Torque Teno Virus Viral Load as a Marker of Immune Function in Allogeneic Haematopoietic Stem Cell Transplantation Recipients
Authors: Mouton William , Conrad Anne , Bal Antonin , Boccard Mathilde , Malcus Christophe , Ducastelle-Lepretre Sophie , Balsat Marie , Barraco Fiorenza , Larcher Marie-Virginie , Fossard Gaëlle , Labussière-Wallet Hélène , Ader Florence , Brengel-Pesce Karen , Trouillet-Assant Sophie ,   Lyon HEMINF Study Group
Published: November 2020

Torque teno virus (TTV) has been proposed as a surrogate biomarker of T-cell function in allogeneic–haematopoietic–stem-cell transplantation (allo-HSCT). Conflicting data exists regarding the value of TTV to assess the degree of immunosuppression. The aim of the present study was to investigate the correlation between TTV viral load and immune function. Using samples from a prospective cohort composed of healthy-volunteers (HV) and allo-HSCT recipients at 6 months post-transplantation, we assessed the correlation between TTV viraemia and immune cell counts or T-cell proliferation capacity post-phytohaemagglutinin stimulation. TTV viraemia was detected in 68% of HV (n = 80) and 100% of allo-HSCT recipients (n = 41; p < 0.001); it was significantly higher in allo-HSCT recipients (3.9 vs. 2.1 Log copies/mL, p < 0.001). There was no correlation between T-cell function and CD3+T-cell count (rho: 0.002) suggesting that T-cell count can normalise without full functional recovery. Furthermore, no significant correlation was observed between TTV viraemia and absolute total/subset lymphocyte counts (rho: <0.13). The highest correlation was observed between TTV viral load and T-cell proliferation capacity (rho: −0.39). We therefore report an inverse correlation between T-cell function and TTV viraemia that is independent of T-cell count. Monitoring of TTV viraemia could be a fast suitable option to objectively assess the competence of immune function in at-risk populations.
Significance of Viral Activity for Regulating Heterotrophic Prokaryote Community Dynamics along a Meridional Gradient of Stratification in the Northeast Atlantic Ocean
Authors: Mojica Kristina D. A. , Brussaard Corina P. D.
Published: November 2020

How microbial populations interact influences the availability and flux of organic carbon in the ocean. Understanding how these interactions vary over broad spatial scales is therefore a fundamental aim of microbial oceanography. In this study, we assessed variations in the abundances, production, virus and grazing induced mortality of heterotrophic prokaryotes during summer along a meridional gradient in stratification in the North Atlantic Ocean. Heterotrophic prokaryote abundance and activity varied with phytoplankton biomass, while the relative distribution of prokaryotic subpopulations (ratio of high nucleic acid fluorescent (HNA) and low nucleic acid fluorescent (LNA) cells) was significantly correlated to phytoplankton mortality mode (i.e., viral lysis to grazing rate ratio). Virus-mediate morality was the primary loss process regulating the heterotrophic prokaryotic communities (average 55% of the total mortality), which may be attributed to the strong top-down regulation of the bacterivorous protozoans. Host availability, encounter rate, and HNA:LNA were important factors regulating viral dynamics. Conversely, the abundance and activity of bacterivorous protozoans were largely regulated by temperature and turbulence. The ratio of total microbial mediated mortality to total available prokaryote carbon reveals that over the latitudinal gradient the heterotrophic prokaryote community gradually moved from a near steady state system regulated by high turnover in subtropical region to net heterotrophic production in the temperate region.
Flow Virometry Quantification of Host Proteins on the Surface of HIV-1 Pseudovirus Particles
Authors: Burnie Jonathan , Tang Vera A. , Welsh Joshua A. , Persaud Arvin T. , Thaya Laxshaginee , Jones Jennifer C. , Guzzo Christina
Published: November 2020

The HIV-1 glycoprotein spike (gp120) is typically the first viral antigen that cells encounter before initiating immune responses, and is often the sole target in vaccine designs. Thus, characterizing the presence of cellular antigens on the surfaces of HIV particles may help identify new antiviral targets or impact targeting of gp120. Despite the importance of characterizing proteins on the virion surface, current techniques available for this purpose do not support high-throughput analysis of viruses, and typically only offer a semi-quantitative assessment of virus-associated proteins. Traditional bulk techniques often assess averages of viral preparations, which may mask subtle but important differences in viral subsets. On the other hand, microscopy techniques, which provide detail on individual virions, are difficult to use in a high-throughput manner and have low levels of sensitivity for antigen detection. Flow cytometry is a technique that traditionally has been used for rapid, high-sensitivity characterization of single cells, with limited use in detecting viruses, since the small size of viral particles hinders their detection. Herein, we report the detection and surface antigen characterization of HIV-1 pseudovirus particles by light scattering and fluorescence with flow cytometry, termed flow virometry for its specific application to viruses. We quantified three cellular proteins (integrin α4β7, CD14, and CD162/PSGL-1) in the viral envelope by directly staining virion-containing cell supernatants without the requirement of additional processing steps to distinguish virus particles or specific virus purification techniques. We also show that two antigens can be simultaneously detected on the surface of individual HIV virions, probing for the tetraspanin marker, CD81, in addition to α4β7, CD14, and CD162/PSGL-1. This study demonstrates new advances in calibrated flow virometry as a tool to provide sensitive, high-throughput characterization of the viral envelope in a more efficient, quantitative manner than previously reported techniques.
Methamphetamine Increases the Proportion of SIV-Infected Microglia/Macrophages, Alters Metabolic Pathways, and Elevates Cell Death Pathways: A Single-Cell Analysis
Authors: Niu Meng , Morsey Brenda , Lamberty Benjamin G. , Emanuel Katy , Yu Fang , León-Rivera Rosiris , Berman Joan W. , Gaskill Peter J. , Matt Stephanie M. , Ciborowski Pawel S. , Fox Howard S.
Published: November 2020

Both substance use disorder and HIV infection continue to affect many individuals. Both have untoward effects on the brain, and the two conditions often co-exist. In the brain, macrophages and microglia are infectable by HIV, and these cells are also targets for the effects of drugs of abuse, such as the psychostimulant methamphetamine. To determine the interaction of HIV and methamphetamine, we isolated microglia and brain macrophages from SIV-infected rhesus monkeys that were treated with or without methamphetamine. Cells were subjected to single-cell RNA sequencing and results were analyzed by statistical and bioinformatic analysis. In the animals treated with methamphetamine, a significantly increased proportion of the microglia and/or macrophages were infected by SIV. In addition, gene encoding functions in cell death pathways were increased, and the brain-derived neurotropic factor pathway was inhibited. The gene expression patterns in infected cells did not cluster separately from uninfected cells, but clusters comprised of microglia and/or macrophages from methamphetamine-treated animals differed in neuroinflammatory and metabolic pathways from those comprised of cells from untreated animals. Methamphetamine increases CNS infection by SIV and has adverse effects on both infected and uninfected microglia and brain macrophages, highlighting the dual and interacting harms of HIV infection and drug abuse on the brain.
Simplified Bioprinting-Based 3D Cell Culture Infection Models for Virus Detection
Authors: Koban Robert , Lam Tobias , Schwarz Franziska , Kloke Lutz , Bürge Silvio , Ellerbrok Heinz , Neumann Markus
Published: November 2020

Studies of virus–host interactions in vitro may be hindered by biological characteristics of conventional monolayer cell cultures that differ from in vivo infection. Three-dimensional (3D) cell cultures show more in vivo-like characteristics and may represent a promising alternative for characterisation of infections. In this study, we established easy-to-handle cell culture platforms based on bioprinted 3D matrices for virus detection and characterisation. Different cell types were cultivated on these matrices and characterised for tissue-like growth characteristics regarding cell morphology and polarisation. Cells developed an in vivo-like morphology and long-term cultivation was possible on the matrices. Cell cultures were infected with viruses which differed in host range, tissue tropism, cytopathogenicity, and genomic organisation and virus morphology. Infections were characterised on molecular and imaging level. The transparent matrix substance allowed easy optical monitoring of cells and infection even via live-cell microscopy. In conclusion, we established an enhanced, standardised, easy-to-handle bioprinted 3D-cell culture system. The infection models are suitable for sensitive monitoring and characterisation of virus–host interactions and replication of different viruses under physiologically relevant conditions. Individual cell culture models can further be combined to a multicellular array. This generates a potent diagnostic tool for propagation and characterisation of viruses from diagnostic samples.
Identification of Novel Astroviruses in the Gastrointestinal Tract of Domestic Cats
Authors: Van Brussel Kate , Wang Xiuwan , Shi Mang , Carrai Maura , Li Jun , Martella Vito , Beatty Julia , Holmes Edward , Barrs Vanessa
Published: November 2020

Astroviruses, isolated from numerous avian and mammalian species including humans, are commonly associated with enteritis and encephalitis. Two astroviruses have previously been identified in cats, and while definitive evidence is lacking, an association with enteritis is suggested. Using metagenomic next-generation sequencing of viral nucleic acids from faecal samples, we identified two novel feline astroviruses termed Feline astrovirus 3 and 4. These viruses were isolated from healthy shelter-housed kittens (Feline astrovirus 3; 6448 bp) and from a kitten with diarrhoea that was co-infected with Feline parvovirus (Feline astrovirus 4, 6549 bp). Both novel astroviruses shared a genome arrangement of three open reading frames (ORFs) comparable to that of other astroviruses. Phylogenetic analysis of the concatenated ORFs, ORF1a, ORF1b and capsid protein revealed that both viruses were phylogenetically distinct from other feline astroviruses, although their precise evolutionary history could not be accurately determined due to a lack of resolution at key nodes. Large-scale molecular surveillance studies of healthy and diseased cats are needed to determine the pathogenicity of feline astroviruses as single virus infections or in co-infections with other enteric viruses.
Reliability and Efficiency of Pollutant Removal in Four-Stage Constructed Wetland of SSVF-SSHF-SSHF-SSVF Type
Authors: Gizińska-Górna Magdalena , Jóźwiakowski Krzysztof , Marzec Michał
Published: November 2020

The present paper reports pollutant removal efficiencies and reliability for a four-stage hybrid constructed wetland (HCW) consisting of the following sequence of subsurface (SS), vertical flow (VF) and horizontal flow (HF) beds: SSVF-SSHF-SSHF-SSVF. The experiments were carried out over a period of three years, with sampling done in each season: winter, spring, summer and autumn. Grab samples of wastewater collected from different stages of treatment were tested for total suspended solids (TSS), BOD5, COD, total nitrogen (TN) and total phosphorus (TP). The wetland was found to have a very high efficiency of removal of suspended solids and organics, with relatively little seasonal variability. The three-year average TSS removal efficiency was approximately 92.7%. The effectiveness of elimination of organic compounds was very high throughout the study period at 96.6% BOD5 and 95% COD. The effluent from the four-stage system had significantly lower contents of TN and TP compared to the wastewater discharged from the first two beds of this facility.
BTX Removal from Open Aqueous Systems by Modified Cellulose Fibers and Evaluation of Competitive Evaporation Kinetics
Authors: Tursi Antonio , Chidichimo Francesco , Bagetta Rita , Beneduci Amerigo
Published: November 2020

BTX stands for Benzene, Toluene, and Xylenes, which are volatile organic compounds contained in petroleum products such as gasoline. They have negative health effects and are sadly known for soil, air, and water contamination. This paper provides an investigation on BTX removal from open water systems like those represented by natural water bodies. In such systems, the evaporation process takes place, stealing the pollutants from the aqueous matrix by transferring them into the air, resulting in a secondary pollution. To prevent this situation, adsorption of these organic compounds on cellulose fibers, extracted from Spanish Broom vegetable, was studied. Raw and surface modified cellulose fibers were used for this purpose. The second ones were hydrophobized by two different green and low-cost functionalization systems (no solvent urethane functionalization and low pressure plasma treatments). Batch experiments were performed in an open system where BTX underwent two competing removing mechanisms: volatilization, and adsorption/desorption on/from the fibers dispersed in the water system. A mathematical model was implemented for the interpretation of the observed time-varying pollutant concentrations and the estimation of the kinetic constants for adsorption, desorption, and evaporation. The developed model, provided with the aforementioned parameters calibrated for each type of fibers, was then used for the prediction of their adsorption capacities both into open and closed systems.
Investigation of Hydraulic Performance Based on Response Surface Methodology for an Agricultural Chemigation Proportional Injector
Authors: Tang Pan , Chen Chao , Li Hong
Published: November 2020

Injectors are key pieces of equipment for chemigation systems, and their hydraulic performance has a significant effect on chemigation systems and crops. In order to investigate the influence of different working parameters on hydraulic performance for a water-powered proportional injector (PI), three key parameters of inlet and injection flow rate were researched using a one-factor experimental design method. The regression equations between different factors and response variables were established through a response surface method based on one-factor experimental results. Lastly, a mathematical model of the actual injection ratio was established. Some experiments under different, randomly selected parameter combinations were carried out to verify the prediction precision of the mathematical mode. The results showed that the injection flow rate increased first within the differential pressure of 0.05 to 0.10 MPa and then tended towards stability with increasing differential pressure. The injection flow rate decreased by increasing the viscosity and the change in the injection flow rate was small enough when the viscosity was greater than 500 mPa·s. The impact factors, in order of significance, for inlet flow rate were differential pressure, viscosity of injection liquid and setting injection ratio. The impact factors, in order of significance, for injection flow rate were viscosity of injection liquid, setting injection ratio and differential pressure. The regressive model for predicting the actual injection ratio was validated using an experiment and the relative deviation between calculated value and tested value was less than 5.98%, which indicated that the mathematical model had high credibility.
Eremitis berbertii and E. fluminensis (Poaceae, Bambusoideae): New Species from the Brazilian Atlantic Forest and Updates on Leaf Microcharacters in the Genus
Authors: Ferreira Fabrício Moreira , Silva Christian , Welker Cassiano A. D. , Dórea Marcos C. , Leite Kelly Regina B. , Clark Lynn G. , Oliveira Reyjane P.
Published: November 2020

Eremitis Döll is a herbaceous bamboo genus (Poaceae, Bambusoideae, Olyreae) belonging to subtribe Parianinae that currently includes seven species. During fieldwork conducted throughout the geographic distribution of the genus, two new species with blue iridescent leaves, E. berbertii F. M. Ferreira & R. P. Oliveira and E. fluminensis F. M. Ferreira & R. P. Oliveira, from the Brazilian Atlantic forest, were collected and are here described and illustrated. Eremitis berbertii is endemic to the Private Reserve of Natural Heritage (Reserva Particular do Patrimônio Natural [RPPN]) Serra do Teimoso, in Bahia State, and E. fluminensis is restricted to the south of Rio de Janeiro State. In this study, micromorphological features of the leaf epidermis of the new species were compared with other species of the genus that display blue iridescence in their leaf blades. Variegated leaves are recorded for the first time in herbaceous bamboos. Comments on habitat, distribution, and conservation status of the new taxa are provided, as well as a revised identification key to all species of the genus described so far.
MATERIALS FOR THE TOPONIMIC DICTIONARY OF THE DIALECT OF THE VILLAGE LOYMA IN THE PRILUZSKY DISTRICT OF THE REPUBLIC OF KOMI
Authors: Lesnikov S.V.
Published: November 2020

He article reviews the composition and main level of names (including placenames, activity, oronyms, hydronyms and microtoponyms different) vocabulary early emigrant dialect of the Vologda-Vyatka group of Northern Russian dialects.
GEOGRAPHICAL TERMS BALKA/SALA IN TOPONIMIA OF THE SOUTH OF RUSSIAON
Authors: Kichikova N.A. , Dzhambinova N.C.
Published: November 2020

The article discusses the functioning of the geographical terms balka/sala, which play an important role in the formation of the toponymy of the regions of the South of Russia. The analysis showed that these terms are actively involved in the formation of the toponymic system of the South of Russia, which is due to the common landscape and the commonality of geographical appellatives functioning in this territory, which are part of toponyms of different categories.
THE ROLE OF ENVIRONMENTAL TEMPERATURE FLUCTUATION IN THE NATURAL REGULATION OF FOREST STATE
Authors: Krutskih Yuliya , Kamalova Nina , Matveev Nikolai
Published: November 2020

The paper analyzes meteorological data on the maximum daily temperature of the day (noon) and evening (at sunset) in detail. Based on the analysis, a verbal model of the influence of the planetary motion of the Earth and the water cycle in nature on temperature fluctuations and, consequently, on the state of forests is formed. Then a formal model of these influences is presented, that is in good agreement with experiment at a certain computer simulation.
Prototipo de reconocimiento facial para mejorar el control de asistencia de estudiantes en UNIANDES, Quevedo
Authors: Lara-Jacho Steven Bryan , Albarracín-Zambrano Luis Orlando , Ponce-Ruiz Dionisio Vitalio
Published: November 2020

La investigación tiene por objetivo diseñar un prototipo de reconocimiento facial para mejorar el control de asistencia de estudiantes en UNIANDES – Quevedo, para la identificación facial de los estudiantes al laboratorio de cómputo. El uso de este prototipo logró automatizar el proceso de registro de asistencia a clases de los docentes y estudiantes en los laboratorios de cómputo mediante el reconocimiento facial, facilitando esta tarea con rapidez y exactitud. El docente se coloca frente a la cámara que está en la parte de afuera del laboratorio de cómputo, por reconocimiento facial se activa el dispositivo que abre la puerta, enciende el aire acondicionado y las luces. Posteriormente los estudiantes se colocan uno por uno frente a la cámara que realiza la detección del rostro comparándolos con los almacenados en la base de datos.