bluuebunny/crossref_metadata_2025 huggingface dataset:
A ∼28-kyr Continuous Lacustrine Paleoseismic Record of the Intraplate, Slow-Slipping Fuyun Fault in Northwest China
Authors: Fan Jiawei , Xu Hongyan , Shi Wei , Guo Qiaoqiao , Zhang Siqi , Wei Xiaotong , Cai Minggang , Huang Shuaitang , Wang Jiangyong , Xiao Jule
Published: January 2022

The Fuyun Fault is a typical intraplate, slow-slipping fault, but has been repeatedly ruptured by surface wave magnitude (Ms) ≥ 8.0 earthquakes. The 11 August 1931 Ms 8.0 Fuyun earthquake resulted in more than 10,000 casualties in the sparsely populated Fuyun area. Cosmogenic 10Be dating of offset landforms produced by prehistoric Ms ≥ 8.0 earthquakes yields an average recurrence interval of 9,700 ± 3,300 years, much longer than previously estimated 2,000–4,500 years, clouding our understanding of the timing and recurrence behavior of past earthquakes originating from the Fuyun Fault. Reflection seismic data reveal widely distributed subaquatic faults in Yileimu Lake, implying high sensitivity of the lake sediments to paleoearthquakes originating from the Fuyun Fault. Two new long sediment cores (Y20A: 267 cm; Y20B: 890 cm) together with previously published two short cores (Y19: 71 cm; Y20: 31.5 cm) from the depocenter and nearshore zone of Yileimu Lake are used for stratigraphic correlations and analyses of sedimentary structures, grain-size distributions, magnetic susceptibility, elemental composition and carbon content. The mass-wasting deposits with underlying soft-sediment deformation structures (SSDS) in the Y20B core indicate six siliciclastic-enriched sandy sediment fluxes from earthquake-triggered landslides of granitic rocks, and isolated SSDS record three additional earthquake-induced in situ deformations. Turbidite-like deposits with sorting indices >3 and Si contents >700 counts per second (cps) are comparable to those of the seismic mass-wasting deposits, and are thus interpreted as seismites from earthquake-induced re-deposition of nearshore sediments. There are a total of 20 seismic events recorded by the Y20B core. Seismic intensity calculation results, combined with historical seismic data, provide potential magnitudes of Ms ≥ 8.0, Ms ≥ 7.0, and Ms ≥ 5.5 for the earthquake-triggered mass-wasting deposits, SSDS, and turbidite-like seismites, respectively, in Yileimu Lake, generally consistent with previously published magnitude thresholds. Radiocarbon dating and stratigraphic correlations constrain the timing of these past earthquakes to ∼28 cal kyr BP. This unique, long lacustrine paleoseismic record suggests a weakly periodic pattern with recurrence intervals between 2,317 and 7,830 years and an average of 5,303 years for potential Ms ≥ 8.0 earthquakes, and reveals an unprecedented high frequency of potential Ms ≥ 7.0 earthquakes originating from the Fuyun Fault in the last 5 kyr, demonstrating the urgent need for an improved assessment of seismic hazards and risks in the Fuyun Fault zone.
Career Competencies for Academic Career Progression: Experiences of Academics at a South African University
Authors: Barnes Nina , du Plessis Marieta , Frantz José
Published: January 2022

An understanding of career competencies is critical for the progression of academic careers, as it influences the availability of adequate and capable academic staff at all levels within universities. The study aimed to explore and describe the career competencies that academics demonstrate to successfully progress in their careers, while theoretically underpinned by an integrated competency framework. This report is based on the qualitative experiences, gathered through semi-structured interviews of eight academic staff in various career phases, in a South African university. Data was thematically analysed, while a deductive modality was adopted to identify the competencies. The findings align very closely with the dimensions of the integrated competency framework, including reflective competencies: gap analysis, self-evaluation, social comparison, and goal orientation; communicative competencies: information seeking and negotiation; and behavioural competencies: strategy alignment, control and agency, university awareness, continuous learning and collaboration. Whilst the study did not include a comparative analysis, it is interesting to note that strategy alignment was the most commonly found competency, with negotiation only demonstrated by more senior academics. A career competency approach provides leaders and development practitioners in the higher education sector with factors to consider, as they attempt to understand holistic development for academic career progression. Moreover, how to assist and support the development of academic career progression. It offers academics a keen awareness, as a personal resource, to engage and navigate self-directed career management behaviour.
Genetic Insights Into the Introduction History of Black Rats Into the Eastern Indian Ocean
Authors: Thomson Vicki A. , Wiewel Andrew S. , Palmer Russell , Hamilton Neil , Algar Dave , Pink Caitlyn , Mills Harriet , Aplin Ken P. , Clark Geoffrey , Anderson Atholl , Herrera Michael B. , Myers Steven , Bertozzi Terry , Piper Philip J. , Suzuki Hitoshi , Donnellan Steve
Published: January 2022

Islands can be powerful demonstrations of how destructive invasive species can be on endemic faunas and insular ecologies. Oceanic islands in the eastern Indian Ocean have suffered dramatically from the impact of one of the world’s most destructive invasive species, the black rat, causing the loss of endemic terrestrial mammals and ongoing threats to ground-nesting birds. We use molecular genetic methods on both ancient and modern samples to establish the origins and minimum invasion frequencies of black rats on Christmas Island and the Cocos-Keeling Islands. We find that each island group had multiple incursions of black rats from diverse geographic and phylogenetic sources. Furthermore, contemporary black rat populations on these islands are highly admixed to the point of potentially obscuring their geographic sources. These hybridisation events between black rat taxa also pose potential dangers to human populations on the islands from novel disease risks. Threats of ongoing introductions from yet additional geographic sources is highlighted by genetic identifications of black rats found on ships, which provides insight into how recent ship-borne human smuggling activity to Christmas Island can negatively impact its endemic species.
An Augmented High-Dimensional Graphical Lasso Method to Incorporate Prior Biological Knowledge for Global Network Learning
Authors: Zhuang Yonghua , Xing Fuyong , Ghosh Debashis , Banaei-Kashani Farnoush , Bowler Russell P. , Kechris Katerina
Published: January 2022

Biological networks are often inferred through Gaussian graphical models (GGMs) using gene or protein expression data only. GGMs identify conditional dependence by estimating a precision matrix between genes or proteins. However, conventional GGM approaches often ignore prior knowledge about protein-protein interactions (PPI). Recently, several groups have extended GGM to weighted graphical Lasso (wGlasso) and network-based gene set analysis (Netgsa) and have demonstrated the advantages of incorporating PPI information. However, these methods are either computationally intractable for large-scale data, or disregard weights in the PPI networks. To address these shortcomings, we extended the Netgsa approach and developed an augmented high-dimensional graphical Lasso (AhGlasso) method to incorporate edge weights in known PPI with omics data for global network learning. This new method outperforms weighted graphical Lasso-based algorithms with respect to computational time in simulated large-scale data settings while achieving better or comparable prediction accuracy of node connections. The total runtime of AhGlasso is approximately five times faster than weighted Glasso methods when the graph size ranges from 1,000 to 3,000 with a fixed sample size (n = 300). The runtime difference between AhGlasso and weighted Glasso increases when the graph size increases. Using proteomic data from a study on chronic obstructive pulmonary disease, we demonstrate that AhGlasso improves protein network inference compared to the Netgsa approach by incorporating PPI information.
Analysis and Visualization of Spatial Transcriptomic Data
Authors: Liu Boxiang , Li Yanjun , Zhang Liang
Published: January 2022

Human and animal tissues consist of heterogeneous cell types that organize and interact in highly structured manners. Bulk and single-cell sequencing technologies remove cells from their original microenvironments, resulting in a loss of spatial information. Spatial transcriptomics is a recent technological innovation that measures transcriptomic information while preserving spatial information. Spatial transcriptomic data can be generated in several ways. RNA molecules are measured by in situ sequencing, in situ hybridization, or spatial barcoding to recover original spatial coordinates. The inclusion of spatial information expands the range of possibilities for analysis and visualization, and spurred the development of numerous novel methods. In this review, we summarize the core concepts of spatial genomics technology and provide a comprehensive review of current analysis and visualization methods for spatial transcriptomics.
MIMRDA: A Method Incorporating the miRNA and mRNA Expression Profiles for Predicting miRNA-Disease Associations to Identify Key miRNAs (microRNAs)
Authors: Li Xianbin , Ai Hannan , Li Bizhou , Zhang Chaohui , Meng Fanmei , Ai Yuncan
Published: January 2022

Identifying cancer-related miRNAs (or microRNAs) that precisely target mRNAs is important for diagnosis and treatment of cancer. Creating novel methods to identify candidate miRNAs becomes an imminent Frontier of researches in the field. One major obstacle lies in the integration of the state-of-the-art databases. Here, we introduce a novel method, MIMRDA, which incorporates the miRNA and mRNA expression profiles for predicting miRNA-disease associations to identify key miRNAs. As a proof-of-principle study, we use the MIMRDA method to analyze TCGA datasets of 20 types (BLCA, BRCA, CESE, CHOL, COAD, ESCA, HNSC, KICH, KIRC, KIRP, LIHC, LUAD, LUSC, PAAD, PRAD, READ, SKCM, STAD, THCA and UCEC) of cancer, which identified hundreds of top-ranked miRNAs. Some (as Category 1) of them are endorsed by public databases including TCGA, miRTarBase, miR2Disease, HMDD, MISIM, ncDR and mTD; others (as Category 2) are supported by literature evidences. miR-21 (representing Category 1) and miR-1258 (representing Category 2) display the excellent characteristics of biomarkers in multi-dimensional assessments focusing on the function similarity analysis, overall survival analysis, and anti-cancer drugs’ sensitivity or resistance analysis. We compare the performance of the MIMRDA method over the Limma and SPIA packages, and estimate the accuracy of the MIMRDA method in classifying top-ranked miRNAs via the Random Forest simulation test. Our results indicate the superiority and effectiveness of the MIMRDA method, and recommend some top-ranked key miRNAs be potential biomarkers that warrant experimental validations.
A Single Dose of an MVA Vaccine Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Protein Neutralizes Variants of Concern and Protects Mice From a Lethal SARS-CoV-2 Infection
Authors: Pérez Patricia , Lázaro-Frías Adrián , Zamora Carmen , Sánchez-Cordón Pedro J. , Astorgano David , Luczkowiak Joanna , Delgado Rafael , Casasnovas José M. , Esteban Mariano , García-Arriaza Juan
Published: January 2022

We generated an optimized COVID-19 vaccine candidate based on the modified vaccinia virus Ankara (MVA) vector expressing a full-length prefusion-stabilized SARS-CoV-2 spike (S) protein, termed MVA-CoV2-S(3P). The S(3P) protein was expressed at higher levels (2-fold) than the non-stabilized S in cells infected with the corresponding recombinant MVA viruses. One single dose of MVA-CoV2-S(3P) induced higher IgG and neutralizing antibody titers against parental SARS-CoV-2 and variants of concern than MVA-CoV2-S in wild-type C57BL/6 and in transgenic K18-hACE2 mice. In immunized C57BL/6 mice, two doses of MVA-CoV2-S or MVA-CoV2-S(3P) induced similar levels of SARS-CoV-2-specific B- and T-cell immune responses. Remarkably, a single administration of MVA-CoV2-S(3P) protected all K18-hACE2 mice from morbidity and mortality caused by SARS-CoV-2 infection, reducing SARS-CoV-2 viral loads, histopathological lesions, and levels of pro-inflammatory cytokines in the lungs. These results demonstrated that expression of a novel full-length prefusion-stabilized SARS-CoV-2 S protein by the MVA poxvirus vector enhanced immunogenicity and efficacy against SARS-CoV-2 in animal models, further supporting MVA-CoV2-S(3P) as an optimized vaccine candidate for clinical trials.
Pleiotropic Effect of IL-6 Produced by B-Lymphocytes During Early Phases of Adaptive Immune Responses Against TB Infection
Authors: Linge Irina , Tsareva Anastasiya , Kondratieva Elena , Dyatlov Alexander , Hidalgo Juan , Zvartsev Ruslan , Apt Alexander
Published: January 2022

The role of B cells migrating to the lung and forming follicles during tuberculosis (TB) inflammation is still the subject of debate. In addition to their antibody production and antigen-presenting functions, B cells secrete different cytokines and chemokines, thus participating in complex networks of innate and adaptive immunity. Importantly, lung B-cells produce high amounts of the pleiotropic gp130 cytokine IL-6. Its role during TB infection remains controversial, partly due to the fact that IL-6 is produced by different cell types. To investigate the impact of IL-6 produced by B cells on TB susceptibility and immune responses, we established a mouse strain with specific IL-6 deficiency in B cells (CD19cre-IL-6fl/fl, B-IL-6KO) on the B6 genetic background. Selective abrogation of IL-6 in B cells resulted in shortening the lifespan of TB-infected B-IL-6KO mice compare to the wild-type controls. We provide evidence that at the initial TB stages B cells serve as a critical source of IL-6. In the lung, the effect of IL-6 deficiency in B cells is associated rather with B and T cell functioning, than with macrophage polarization. TB-infected B-IL-6KO mice displayed diminished sizes of B cells themselves, CD4+IFN-γ+, Th17+, and CD4+CXCR5+follicular T cell populations. The pleiotropic effect of B-cell-derived IL-6 on T-cells demonstrated in our study bridges two major lymphocyte populations and sheds some light on B- and T-cells interactions during the stage of anti-TB response when the host switches on a plethora of acquired immune reactions.
Multiplex Antibody Analysis of IgM, IgA and IgG to SARS-CoV-2 in Saliva and Serum From Infected Children and Their Close Contacts
Authors: Dobaño Carlota , Alonso Selena , Vidal Marta , Jiménez Alfons , Rubio Rocío , Santano Rebeca , Barrios Diana , Pons Tomas Gemma , Melé Casas María , Hernández García María , Girona-Alarcón Mònica , Puyol Laura , Baro Barbara , Millat-Martínez Pere , Ajanovic Sara , Balanza Núria , Arias Sara , Rodrigo Melero Natalia , Carolis Carlo , García-Miquel Aleix , Bonet-Carné Elisenda , Claverol Joana , Cubells Marta , Fortuny Claudia , Fumadó Victoria , Codina Anna , Bassat Quique , Muñoz-Almagro Carmen , Fernández de Sevilla Mariona , Gratacós Eduard , Izquierdo Luis , García-García Juan José , Aguilar Ruth , Jordan Iolanda , Moncunill Gemma
Published: January 2022

COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the infectivity dynamics of SARS-CoV-2 in pediatric populations and guide public health interventions, particularly if this population is not fully vaccinated. We evaluated the utility of high-throughput Luminex assays to quantify saliva IgM, IgA and IgG antibodies against five SARS-CoV-2 spike (S) and nucleocapsid (N) antigens in a contacts and infectivity longitudinal study in 122 individuals (52 children and 70 adults). We compared saliva versus serum/plasma samples in infected children and adults diagnosed by weekly RT-PCR over 35 days (n=62), and those who consistently tested negative over the same follow up period (n=60), in the Summer of 2020 in Barcelona, Spain. Saliva antibody levels in SARS-CoV-2 RT-PCR positive individuals were significantly higher than in negative individuals and correlated with those measured in sera/plasmas. Asymptomatic infected individuals had higher levels of anti-S IgG than symptomatic individuals, suggesting a protective anti-disease role for antibodies. Higher anti-S IgG and IgM levels in serum/plasma and saliva, respectively, in infected children compared to infected adults could also be related to stronger clinical immunity in them. Among infected children, males had higher levels of saliva IgG to N and RBD than females. Despite overall correlation, individual clustering analysis suggested that responses that may not be detected in blood could be patent in saliva, and vice versa.

In conclusion, measurement of SARS-CoV-2-specific saliva antibodies should be considered as a complementary non-invasive assay to serum/plasma to determine COVID-19 prevalence and transmission in pediatric populations before and after vaccination campaigns.
HLA-G: Too Much or Too Little? Role in Cancer and Autoimmune Disease
Authors: Martín-Villa José Manuel , Vaquero-Yuste Christian , Molina-Alejandre Marta , Juarez Ignacio , Suárez-Trujillo Fabio , López-Nares Adrián , Palacio‐Gruber José , Barrera-Gutiérrez Luis , Fernández-Cruz Eduardo , Rodríguez-Sainz Carmen , Arnaiz-Villena Antonio
Published: January 2022

HLA-G is a non-classical HLA class I molecule with immunomodulatory properties. It was initially described at the maternal-fetal interface, and it was later found that this molecule was constitutively expressed on certain immuneprivileged tissues, such as cornea, endothelial and erythroid precursors, and thymus. The immunosuppressive effect of HLA-G is exerted through the interaction with its cognate receptors, expressed on immunocompetent cells, like ILT2, expressed on NK, B, T cells and APCs; ILT4, on APCs; KIR, found on the surface of NK cells; and finally, the co-receptor CD8. Because of these immunomodulatory functions, HLA-G has been involved in several processes, amongst which organ transplantation, viral infections, cancer progression, and autoimmunity. HLA-G neo-expression on tumors has been recently described in several types of malignancies. In fact, tumor progression is tightly linked to the presence of the molecule, as it exerts its tolerogenic function, inhibiting the cells of the immune system and favoring tumor escape. Several polymorphisms in the 3’UTR region condition changes in HLA-G expression (14bp and +3142C/G, among others), which have been associated with both the development and outcome of patients with different tumor types. Also, in recent years, several studies have shown that HLA-G plays an important role in the control of autoimmune diseases. The ability of HLA-G to limit the progression of these diseases has been confirmed and, in fact, levels of the molecule and several of its polymorphisms have been associated with increased susceptibility to the development of autoimmune diseases, as well as increased disease severity. Thus, modulating HLA-G expression in target tissues of oncology patients or patients with autoimmune diseases may be potential therapeutic approaches to treat these pathological conditions.
Immunogenomic Landscape in Breast Cancer Reveals Immunotherapeutically Relevant Gene Signatures
Authors: Wang Tao , Li Tianye , Li Baiqing , Zhao Jiahui , Li Zhi , Sun Mingyi , Li Yan , Zhao Yanjiao , Zhao Shidi , He Weiguang , Guo Xiao , Ge Rongjing , Wang Lian , Ding Dushan , Liu Saisai , Min Simin , Zhang Xiaonan
Published: January 2022

Breast cancer is characterized by some types of heterogeneity, high aggressive behaviour, and low immunotherapeutic efficiency. Detailed immune stratification is a prerequisite for interpreting resistance to treatment and escape from immune control. Hence, the immune landscape of breast cancer needs further understanding. We systematically clustered breast cancer into six immune subtypes based on the mRNA expression patterns of immune signatures and comprehensively depicted their characteristics. The immunotherapeutic benefit score (ITBscore) was validated to be a superior predictor of the response to immunotherapy in cohorts from various datasets. Six distinct immune subtypes related to divergences in biological functions, signatures of immune or stromal cells, extent of the adaptive immune response, genomic events, and clinical prognostication were identified. These six subtypes were characterized as immunologically quiet, chemokine dominant, lymphocyte depleted, wounding dominant, innate immune dominant, and IFN-γ dominant and exhibited features of the tumor microenvironment (TME). The high ITBscore subgroup, characterized by a high proportion of M1 macrophages:M2 macrophages, an activated inflammatory response, and increased mutational burden (such as mutations in TP53, CDH1 and CENPE), indicated better immunotherapeutic benefits. A low proportion of tumor-infiltrating lymphocytes (TILs) and an inadequate response to immune treatment were associated with the low ITBscore subgroup, which was also associated with poor survival. Analyses of four cohorts treated with immune checkpoint inhibitors (ICIs) suggested that patients with a high ITBscore received significant therapeutic advantages and clinical benefits. Our work may facilitate the understanding of immune phenotypes in shaping different TME landscapes and guide precision immuno-oncology and immunotherapy strategies.
Role of ROS-Induced NLRP3 Inflammasome Activation in the Formation of Calcium Oxalate Nephrolithiasis
Authors: Liu Yunlong , Sun Yan , Kang Juening , He Ziqi , Liu Quan , Wu Jihua , Li Derong , Wang Xiang , Tao Zhiwei , Guan Xiaofeng , She Wusheng , Xu Hua , Deng Yaoliang
Published: January 2022

Calcium oxalate nephrolithiasis is a common and highly recurrent disease in urology; however, its precise pathogenesis is still unknown. Recent research has shown that renal inflammatory injury as a result of the cell-crystal reaction plays a crucial role in the development of calcium oxalate kidney stones. An increasing amount of research have confirmed that inflammation mediated by the cell-crystal reaction can lead to inflammatory injury of renal cells, promote the intracellular expression of NADPH oxidase, induce extensive production of reactive oxygen species, activate NLRP3 inflammasome, discharge a great number of inflammatory factors, trigger inflammatory cascading reactions, promote the aggregation, nucleation and growth process of calcium salt crystals, and ultimately lead to the development of intrarenal crystals and even stones. The renal tubular epithelial cells (RTECs)-crystal reaction, macrophage-crystal reaction, calcifying nanoparticles, endoplasmic reticulum stress, autophagy activation, and other regulatory factors and mechanisms are involved in this process.
Geographies of Dirty Water: Landscape-Scale Inequities in Coastal Access in Rhode Island
Authors: Twichell Julia H. , Mulvaney Kate K. , Merrill Nathaniel H. , Bousquin Justin J.
Published: January 2022

Across the United States, development, gentrification, and water quality degradation have altered our access to the coasts, redistributing the benefits from those spaces. Building on prior coastal and green space access research, we examined different populations’ relative travel distances to all public coastal access and to public marine swimming beaches across the state of Rhode Island, by race, ethnicity, and socioeconomics. Next, we assessed relative travel distances to high quality public coastal amenities, i.e., sites with no history of water quality impairment. We used three state-level policy attributes to identify sites with the best water quality: Clean Water Act Section 303(d) impaired waters, shellfishing restrictions, and bacterial beach closure histories. Our analysis revealed statewide disparities in access to Rhode Island’s public coastal amenities. With robust socioeconomic and geographic controls, race and ethnicity remained strongly correlated to travel distance. Higher proportions of Black and Latinx populations in census block groups were associated with longer travel distances to public access, in particular to public coastal sites with better water quality and to public swimming beaches. This translates to added costs on each trip for areas with higher Black and Latinx populations.
Reoxygenation of the Hypoxia in the East China Sea: A Ventilation Opening for Marine Life
Authors: Chen Chung-Chi , Ko Dong S. , Gong Gwo-Ching , Lien Chun-Chi , Chou Wen-Chen , Lee Hung-Jen , Shiah Fuh-Kwo , Huang Yu-Sin Wita
Published: January 2022

Hypoxia and upwelling co-occur in the summer, and well-mixed water typically reaches the subsurface in the East China Sea (ECS), especially off the Changjiang River estuary. The impact of upwelling on hypoxia and, therefore, on the ecosystem in the ECS is not known. This study demonstrates both positive and negative effects of upwelling on hypoxia and its impact on the ecosystem. With upwelling, the spatial extent of hypoxic water increases with a lower pH but waters with high regenerated nutrients and fugacity of CO2 (fCO2), which are normally confined to the near-bottom, are found just up to 5–10 m below the surface. This upwelled high nutrient water can enhance phytoplankton growth in this region. On one occasion in August 2014, upwelling reached to the surface and lasted for 3 weeks, with the area of coverage ranging from 326.8 to 24,368.0 km2. During this event, the water was mixed thoroughly throughout the water column, with high concentrations of nutrients, chlorophyll a, and slightly undersaturated dissolved oxygen but saturated fCO2, alongside a normal pH. This event may have served as an important pathway from the ocean to the atmosphere for the regenerated CO2. It also provided a productive and suitable environment for marine life and ventilation to alleviate low-oxygen stress in this hypoxic but upwelling region in the ECS.
Structure and Conductivity Correlation in NASICON Based Na3Al2P3O12 Glass: Effect of Na2SO4
Authors: Mandal Indrajeet , Chakraborty Saswata , Ghosh Manasi , Dey Krishna K. , Annapurna K. , Allu Amarnath R.
Published: January 2022

Identifying the factors influencing the movement of sodium cations (Na+) in glasses accelerates the possible options of glass-based solid electrolyte materials for their applications as a promising electrolyte material in sodium-ion batteries. Nevertheless, due to the poor correlation between the structure and conductivity in glass materials, identifying the factors governing the conductivity still exists as a challenging task. Herein, we have investigated the DC-conductivity variations by correlating the structure and conductivity in sodium superionic conductor (NASICON) based Na3Al2P3O12 (NAP) glass (mol%: 37.5 P2O5—25.0 Al2O3—37.5 Na2O) due to the successive substitution of Na2SO4 for Al2O3. Structural variations have been identified using the Raman and magic-angle spinning nuclear magnetic resonance (MAS-NMR) (for 31P, 23Na, and 27Al nuclei) and conductivity measurements have been done using the impedance spectroscopy. From the ac-conductivity spectra, the correlations between mean square displacement (MSD) and dc-conductivity and between the Na+ concentration and dc-conductivity have also been evaluated. Raman spectra reveal that the increase in the Na2SO4 concentration increases the number of isolated SO42− sulfate groups that are charge compensated by the Na+ cations in the NAP glass. MAS-NMR spectra reveal that the increase in Na2SO4 concentration increases the concentration of non-bridging oxygens and further neither S-O-P nor S-O-Al bonds are formed. Impedance spectroscopy reveals that, at 373 K, the DC conductivity of the NAP glass increases with increasing the Na2SO4 up to 7.5 mol% and then decreases with the further increase. In the present study, we have shown that the mobility of sodium cations played a significant role in enhancing the ionic-conductivity. Further, we have shown that inter-ionic Coulombic interactions and the structural modification with the formation of SO42− units significantly influence the critical hopping length < R2 (tp)> of the sodium cations and consequently the mobility and the ionic conductivity. The present study clearly indicates that, based on the compositions, glass materials can also be treated as strong-electrolyte materials.
Bone Marrow-Derived Cells and Wound Age Estimation
Authors: Ishida Yuko , Nosaka Mizuho , Kondo Toshikazu
Published: January 2022

Appropriate technology as well as specific target cells and molecules are key factors for determination of wound vitality or wound age in forensic practice. Wound examination is one of the most important tasks for forensic pathologists and is indispensable to distinguish antemortem wounds from postmortem damage. For vital wounds, estimating the age of the wound is also essential in determining how the wound is associated with the cause of death. We investigated bone marrow-derived cells as promising markers and their potential usefulness in forensic applications. Although examination of a single marker cannot provide high reliability and objectivity in estimating wound age, evaluating the appearance combination of bone marrow-derived cells and the other markers may allow for a more objective and accurate estimation of wound age.
Dietary Effects on Biological Parameters and Gut Microbiota of Harmonia axyridis
Authors: Huang Zhendong , Zhu Li , Lv Jia , Pu Zhanxu , Zhang Lipin , Chen Guoqing , Hu Xiurong , Zhang Zhenyu , Zhang Hongyu
Published: January 2022

The multicolored Asian lady beetle (Harmonia axyridis, H. axyridis, Coleoptera, and Coccinellidae) is an effective biocontrol agent against agricultural pests. Previous studies have suggested that amount, type, and the quality of food can directly affect the biological aspects of H. axyridis. In this study, we investigated the influence of the food sources (Acyrthosiphon pisum Harris, Diaphorina citri Kuwayama, and artificial diets) on the gut microbiota diversity and the biology, reproductive variables, and population growth indicators of H. axyridis. Three kinds of diets were considered in this study: (1) HY: the adult of A. pisum Harris (HY group); (2) HM: the adult of D. citri Kuwayama (HM group); (3) HR: artificial diets prepared by blending a portion of fresh homogenized pork liver (15 g), honey (3 g), distilled water (35 ml) (HR group). We found that gut microbiota composition and diversity and the biological parameters differed when H. axyridis was fed with different diets. The abundance of Enterobacteriaceae was the highest in the HM group, followed by HY group, and was the lowest in the HR group. The abundance of Staphylococcaceae was highest in the HR group. Among the gut fungi, Davidiellaceae and Wallemiaceae were the highest and lowest in the HY group; Incertae_sedis were the major gut fungi in the HR group. Meanwhile, the changes of biological parameters may be correlated with the changes of Streptococcaceae abundance, Micrococcaceae abundance, Staphylococcaceae abundance, and Enterobacteriaceae abundance in responds to diet changes. To sum up, these data suggest that different diets can influence the changes in adult H. axyridis gut microbiota, consequently affecting the biological parameters.
Phage Revolution Against Multidrug-Resistant Clinical Pathogens in Southeast Asia
Authors: Carascal Mark B. , dela Cruz-Papa Donna May , Remenyi Roland , Cruz Mely Cherrylynne B. , Destura Raul V.
Published: January 2022

Southeast Asia (SEA) can be considered a hotspot of antimicrobial resistance (AMR) worldwide. As recent surveillance efforts in the region reported the emergence of multidrug-resistant (MDR) pathogens, the pursuit of therapeutic alternatives against AMR becomes a matter of utmost importance. Phage therapy, or the use of bacterial viruses called bacteriophages to kill bacterial pathogens, is among the standout therapeutic prospects. This narrative review highlights the current understanding of phages and strategies for a phage revolution in SEA. We define phage revolution as the radical use of phage therapy in infectious disease treatment against MDR infections, considering the scientific and regulatory standpoints of the region. We present a three-phase strategy to encourage a phage revolution in the SEA clinical setting, which involves: (1) enhancing phage discovery and characterization efforts, (2) creating and implementing laboratory protocols and clinical guidelines for the evaluation of phage activity, and (3) adapting regulatory standards for therapeutic phage formulations. We hope that this review will open avenues for scientific and policy-based discussions on phage therapy in SEA and eventually lead the way to its fullest potential in countering the threat of MDR pathogens in the region and worldwide.
Polysubstance Use in Early Adulthood: Patterns and Developmental Precursors in an Urban Cohort
Authors: Steinhoff Annekatrin , Bechtiger Laura , Ribeaud Denis , Eisner Manuel P. , Quednow Boris B. , Shanahan Lilly
Published: January 2022

Polysubstance use (i.e., simultaneous or sequential use of different psychoactive substances) is associated with increases in the risk of severe health problems and social impairments. The present study leverages community-representative, long-term longitudinal data from an urban cohort to assess: (a) the prevalence and continuation of polysubstance use between adolescence and early adulthood; (b) different patterns of polysubstance use (i.e., combinations of substances) in early adulthood; and (c) childhood risk factors for polysubstance use in early adulthood. At age 20 (n = 1,180), respondents provided comprehensive self-reported information on past-year substance use, including use of legal and illicit substances (e.g., cannabinoids, stimulants, and hallucinogens), and nonmedical use of prescription drugs (e.g., opioids, tranquilizers). In adolescence (ages 13–17), limited versions of this questionnaire were administered. In childhood (ages 7–11), potential risk factors, including individual-level factors (e.g., sensation-seeking, low self-control, aggression, and internalizing symptoms) and social-environmental factors (e.g., social stressors, exposure to others’ substance use), were assessed. We fitted latent class models to identify classes of participants with different substance use profiles in early adulthood. The results show that polysubstance use increased between early adolescence and early adulthood. The continuation of polysubstance use was common (stability between all adjacent assessments: odds ratio >7). At age 20, more than one-third of participants reported polysubstance use (involving illicit substances, nonmedical use of prescription drugs, and cannabidiol). Four latent classes with polysubstance use were identified: (1) broad spectrum of substances; (2) cannabis and club drugs; (3) cannabis and the nonmedical use of prescription drugs; and (4) different cannabinoids. Risk factors for any polysubstance use included childhood sensation-seeking and exposure to others’ substance use; some childhood risk factors were differentially associated with the four classes (e.g., low self-control in childhood was associated with an increased likelihood of being in the broad spectrum class). The classes also differed with regard to socio-demographic factors. This study revealed that polysubstance use is a widespread and multifaceted phenomenon that typically emerges during adolescence. To facilitate the design of tailored prevention mechanisms, the heterogeneity of polysubstance use and respective socio-demographic and developmental precursors need to be considered.
Which Is a Better Predictor of GFR Decline: 24-h Urine Protein or 24-h Protein–Creatinine Ratio? An Exploration of the MDRD Study Data
Authors: Ayoub Isabelle , Nagaraja Haikady N. , Kang Rima , Rovin Brad , Bhatt Udayan
Published: January 2022

Background

Proteinuria is a known risk factor for progression of chronic kidney disease. Proteinuria magnitude can be estimated by measuring spot urine protein-to-creatinine ratio (least accurate), 24-h urine collection for protein (24 P), or 24-h protein–creatinine ratio (24 PCR). The MDRD study found that 24 P measured at baseline was the strongest single predictor of the rate of GFR decline during study follow-up. However, predictive powers of 24 P and 24 PCR have not been compared in the literature. The current study addresses this question using the MDRD cohort data.

Methods

The study is a retrospective analysis of prospectively collected data from the MDRD cohort using simple and multiple regression models. Slope of measured GFR (mGFR) over time was used as the response and models that included baseline 24 PCR or 24 P were compared for the entire sample and for subgroups formed by restricting the values of 24-h creatinine and 24 P.

Results

Log 24 P and Log 24 PCR correlated almost equally with mGFR slope. However, in simple linear regression models and multivariable linear regression models adjusting for age and sex, the model with 24 PCR had a higher R2 than the corresponding one that had 24 P except for the subgroup 24 P < 1 g.

Conclusion

We observe that 24 PCR may be a better marker of proteinuria magnitude in predicting decline in kidney function compared to 24 P in particular for patients with 24 P ≥ 1. This finding needs validation in prospective clinical trials.
Management of Unruptured Small Multiple Intracranial Aneurysms in China: A Comparative Effectiveness Analysis Based on Real-World Data
Authors: Chen Jigang , Tong Xin , Feng Xin , Peng Fei , Niu Hao , Han Mingyang , Liu Lang , Zhao Yuanli , Wang Daming , Pan Yuesong , Liu Aihua
Published: January 2022

Background

Unruptured small aneurysms with a size of <7 mm were often followed conservatively. However, it is unknown whether unruptured small multiple intracranial aneurysms (MIAs) are better to be prophylactically treated or conservatively followed.

Objective

We aim to compare the effectiveness of different strategies regarding their management.

Methods

A decision-analytic Markov model was built over a entire life cycle. The compared strategies include natural history, treat one aneurysm, treat both aneurysms, annual follow-up, biennial follow-up, and follow-up every 5 years. The inputs for the model were obtained from real-world data and related medical literature. Outcomes were measured in terms of quality-adjusted life-years (QALYs).

Results

Treat both aneurysms had the highest effectiveness of 15.36 QALYs and treat one aneurysm had the second-highest effectiveness of 15.11 QALYs. Probabilistic sensitivity analysis with 10,000 iterations showed that treat both aneurysms and treat one aneurysm were optimal in 67.28 and 17.91% of all cases, respectively. One-way and two-way sensitivity analyses showed that the result was sensitive to the proportion of moderate to severe disability after treating two aneurysms, mortality after treating two aneurysms, proportion of moderate to severe disability after treating one aneurysm, and rupture rate of small growing aneurysm. Either treat both aneurysms or treat one aneurysm would be the optimal strategy under most of the circumstances with the variations of these parameters.

Conclusion

For patients with small unruptured MIAs, prophylactic coiling was superior to conservative management and at least one aneurysm should be treated.
Perspective: Balance Assessments in Progressive Supranuclear Palsy: Lessons Learned
Authors: Dale Marian L. , Prewitt Austin L. , Harker Graham R. , McBarron Grace E. , Mancini Martina
Published: January 2022

Many studies have examined aspects of balance in progressive supranuclear palsy (PSP), but guidance on the feasibility of standardized objective balance assessments and balance scales in PSP is lacking. Balance tests commonly used in Parkinson's disease often cannot be easily administered or translated to PSP. Here we briefly review methodology in prior studies of balance in PSP; then we focus on feasibility by presenting our experience with objective balance assessment in PSP-Richardson syndrome and PSP-parkinsonism during a crossover rTMS intervention trial. We highlight lessons learned, safety considerations, and future approaches for objective balance assessment in PSP.
Differential Tractography and Correlation Tractography Findings on Patients With Mild Traumatic Brain Injury: A Pilot Study
Authors: Li Meng-Jun , Yeh Fang-Cheng , Huang Si-Hong , Huang Chu-Xin , Zhang Huiting , Liu Jun
Published: January 2022

Differential tractography and correlation tractography are new tractography modalities to study neuronal changes in brain diseases, but their performances in detecting neuronal injuries are yet to be investigated in patients with mild traumatic brain injury (mTBI). Here we investigated the white matter injury in mTBI patients using differential and correlation tractography. The diffusion MRI was acquired at 33 mTBI patients and 31 health controls. 7 of the mTBI patients had one-year follow-up scans, and differential tractography was used to evaluate injured fiber bundles on these 7 patients. All subjects were evaluated using digital symbol substitution test (DSST) and trail making test A (TMT-A), and the correlation tractography was performed to explore the exact pathways related to the cognitive performance. Our results showed that differential tractography revealed neuronal changes in the corpus callosum in all 7 follow-up mTBI patients with FDR between 0.007 and 0.17. Further, the correlation tractography showed that the splenium of the corpus callosum, combined with the right superior longitudinal fasciculus and right cingulum, were correlated with DSST (FDR = 0.001669) in the acute mTBI patients. The cognitive impairment findings in the acute stage and the longitudinal findings in the corpus callosum in the chronic stage of mTBI patients suggest that differential tractography and correlation tractography are valuable tools in the diagnostic and prognostic evaluation of neuronal injuries in mTBI patients.
The Italian Observatory on Food Surplus, Recovery, and Waste: The Development Process and Future Achievements
Authors: Grant Federica , Rossi Laura
Published: January 2022

Food loss and waste (FLW) is an environmental, social, and economic problem. Countries all around the world are looking for efficient strategies to prevent and reduce FLW, as recommended by target 12.3 of the Sustainable Development Goals (SDGs) of the United Nations. The European Union (EU) is strongly committed to helping solve the FLW issue, setting up the Platform on Food Losses and Food Waste, and adopting the Farm to Fork Strategy. Italy has also adopted a consolidated approach toward this issue, in particular through the redistribution of food surplus to those in need, a policy that was instituted with the Gadda Law 166/2016. Importantly, this normative framework also provided for the establishment of the National Observatory on Food Surplus, Recovery, and Waste [Osservatorio sulle Eccedenze, i Recuperi e gli Sprechi Alimentari (OERSA)]. This article describes the creation and development of the OERSA, as the technical entity supporting national FLW policies. One of the first actions taken by the OERSA was that of mapping the FLW initiatives that were being implemented along the entire food supply chain in Italy. This gave the OERSA a solid foundation on which to begin working on two different fronts: (1) Collecting data regarding the primary production sector and at the consumer level and (2) Establishing educational programs and awareness campaigns. The data collected by the OERSA highlight that, although several actors of the Italian supply chain are already conscious of the causes of FLW, new strategies that focus on innovation and cooperation should be encouraged.
Pulsed Electric Field-Assisted Extraction of Aroma and Bioactive Compounds From Aromatic Plants and Food By-Products
Authors: Carpentieri Serena , Režek Jambrak Anet , Ferrari Giovanna , Pataro Gianpiero
Published: January 2022

In this work, the effect of pulsed electric field (PEF) pre-treatment on the extractability in green solvents (i. e., ethanol–water mixture and propylene glycol) of target aroma and bioactive compounds, such as vanillin from vanilla pods, theobromine and caffeine from cocoa bean shells, linalool from vermouth mixture, and limonene from orange peels, was investigated. The effectiveness of PEF as a cell disintegration technique in a wide range of field strength (1–5 kV/cm) and energy input (1–40 kJ/kg) was confirmed using impedance measurements, and results were used to define the optimal PEF conditions for the pre-treatment of each plant tissue before the subsequent solid–liquid extraction process. The extracted compounds from untreated and PEF-treated samples were analyzed via GC-MS and HPLC-PDA analysis. Results revealed that the maximum cell disintegration index was detected for cocoa bean shells and vanilla pods (Zp = 0.82), followed by vermouth mixture (Zp = 0.77), and orange peels (Zp = 0.55). As a result, PEF pre-treatment significantly enhanced the extraction yield of the target compounds in both solvents, but especially in ethanolic extracts of vanillin (+14%), theobromine (+25%), caffeine (+34%), linalool (+114%), and limonene (+33%), as compared with untreated samples. Moreover, GC-MS and HPLC-PDA analyses revealed no evidence of degradation of individual compounds due to PEF application. The results obtained in this work suggest that the application of PEF treatment before solid–liquid extraction with green solvents could represent a sustainable approach for the recovery of clean labels and natural compounds from aromatic plants and food by-products.
Tea Tree Oil Terpinen-4-ol Protects Gut Barrier Integrity by Upregulation of Tight Junction Proteins via the ERK1/2-Signaling Pathway
Authors: Yong Yanhong , Fang Biao , Huang Yingxin , Li Junyu , Yu Tianyue , Wu Lianyun , Hu Canying , Liu Xiaoxi , Yu Zhichao , Ma Xingbin , Gooneratne Ravi , Li Sidong , Abd El-Aty A. M. , Ju Xianghong
Published: January 2022

Tea tree oil (TTO) exhibits a potent antioxidant, antibacterial, and anti-inflammatory activity and is commonly used in skincare products. However, it is not clear whether TTO can protect gut barrier damage in inflammatory bowel disease (IBD) patients. Herein, we report the impact of terpinen-4-ol (TER, the primary constituent of TTO), on lipopolysaccharide (LPS)-induced intestinal epithelial cell barrier function impairment in intestinal porcine epithelial cell lines (IPEC-J2) and dextran sulfate sodium (DSS)-induced IBD in mice. TER protected against LPS-induced damage in IPEC-J2 cells in vitro and attenuated DSS-induced colitis in vivo. Added TER promoted the tight junction (TJ) proteins expressing in vitro and in vivo and attenuated the LPS-induced upregulation of ERK phosphorylation in IPEC-J2 cells. However, when an inhibitor of ERK phosphorylation was added, TER did not promote the expression of TJ protein, denoting that the ERK signaling pathway mediates the upregulation of TJ proteins. Our data may propose the potential application of TER in treating IBD.