bluuebunny/crossref_metadata_2025 huggingface dataset:
Ultrasonographic assessment of preoperative gastric volume in patients with dyspepsia: a prospective observational study
Authors: Tan Yuming , Wang Xianchun , Yang Han , Pan Chuanlong , Luo Nanbo , Li Junjie , Yang Fang , Bei Yanling , Cahilog Zhen , Chen Qian , Liu Zhiheng , Yang Xinping
Published: January 2022

Background

Patients undergoing gastroenteroscopy during sedation are prone to aspiration, and most patients with dyspepsia have delayed gastric emptying. This study aimed to investigate the feasibility of measuring the gastric antrum cross-sectional area (CSA) to supply a novel clinical diagnostic reference value in patients with dyspepsia.

Methods

Patients with dyspepsia undergoing elective gastroscopy were included. The Perlas qualitative 0–2 grading scale score was determined before the operation. The anteroposterior diameter (D1) and craniocaudal diameter (D2) between gastric antrum serosal surfaces were measured perpendicular to each other in the supine and right lateral decubitus (RLD) positions. CSA values in the supine position and RLD position were determined. Gastric contents were endoscopically suctioned with the volumes measured and noted as actual gastric volume. Multiple regression analysis was used to fit a mathematical model for estimating the gastric volume. Receiver operating characteristic (ROC) curves were constructed to determine the accuracy of RLD CSA to detect gastric volumes of > 0.8 ml/kg.

Results

A total of 117 patients were enrolled and divided into a functional dyspepsia (FD) group and an organic dyspepsia group according to gastroscopy findings. For a gastric volume of > 0.8 ml/kg, cut-off values for FD and organic dyspepsia were 6.7 cm2 and 10.0 cm2, respectively. Two new modified mathematical models were derived to predict an estimated gastric volume for FD and organic dyspepsia: volume = 3.93 × RLD CSA - 0.47 × age; and volume = 6.15 × RLD CSA - 0.61 × age.

Conclusion

We used the cut-off value of the antral area for the fast diagnosis of gastric volumes in patients with dyspepsia, which may assist clinicians in identifying patients at risk of aspiration.

Trial registration

www.chictr.org.cn (CHICTR-DDD-17010871); registered 15 March 2017.
Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury
Authors: Yin Moli , Nie Yuanwang , Liu Hao , Liu Lei , Tang Lu , Dong Yuan , Hu Chuanmin , Wang Huiyan
Published: January 2022

Background

AKI is related to severe adverse outcomes and mortality with Coronavirus Disease 2019 (COVID-19) patients, that early diagnosed and intervened is imperative. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising biomarkers for detection of acute kidney injury (AKI), but current detection methods are inadequacy, so more rapid, convenient and accuracy methods are needed to detect NGAL for early diagnosis of AKI. Herein, we established a rapid, reliable and accuracy lateral flow immunoassay (LFIA) based on europium nanoparticles (EU-NPS) for the detection of NGAL in human urine specimens.

Methods

A double-antibody sandwich immunofluorescent assay using europium doped nanoparticles was employed and the NGAL monoclonal antibodies (MAbs) conjugate as labels were generated by optimizing electric fusion parameters. Eighty-three urine samples were used to evaluate the clinical application efficiency of this method.

Results

The quantitative detection range of NGAL in AKI was 1-3000 ng/mL, and the detection sensitization was 0.36 ng/mL. The coefficient of variation (CV) of intra-assay and inter-assay were 2.57-4.98 % and 4.11-7.83 %, respectively. Meanwhile, the correlation coefficient between europium nanoparticles-based lateral fluorescence immunoassays (EU-NPS-LFIA) and ARCHITECT analyzer was significant (R2 = 0.9829,n = 83,p < 0.01).

Conclusions

Thus, a faster and easier operation quantitative assay of NGAL for AKI has been established, which is very important and meaningful to diagnose the early AKI, suggesting that the assay can provide an early warning of final outcome of disease.
Simple and effective serum biomarkers potential for predicting status epilepticus in anti-N-methyl-D-aspartate receptor encephalitis
Authors: Liu Yingying , Ma Lili , Ma Xiaomeng , Ma Xueying , Li Jing , Li Donghong , Lin Xiuli , Su Zhumin , Chen Xiaohong
Published: January 2022

Background

Patients with anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis who also present with status epilepticus (SE) often have a poor prognosis. The aim of this study is to explore simple and effective predictors for anti-NMDAR encephalitis accompanied with SE.

Methods

We retrospectively analyzed 65 anti-NMDAR encephalitis patients from January 2015 to December 2018 who admitted to the Third Affiliated Hospital of Sun Yat-sen University. Patients were divided into SE group and non-SE groups. Their pre-treatment data and 3-month follow-up data were retrospectively analyzed.

Results

The results showed that compared with the non-SE group, the levels of serum uric acid (UA) and high-density lipoprotein cholesterol (HDL-C) in anti-NMDAR encephalitis patients with SE decreased significantly before treatment. Additionally, the levels of serum UA and HDL-C increased while the level of C-reactive protein (CRP) decreased 3 months after treatment in the SE group. Compared with the non-SE group, the SE patients had higher modified Rankin scale (mRS) scores before (mRS1) and after treatment (mRS2). Serum UA concentrations before treatment showed significantly negative correlations with mRS1 (r = − 0.407, p < 0.01) and mRS2 (r = − 0.458, p < 0.001), while the level of serum CRP before treatment had strong positive correlations with mRS1 (r = 0.304, p < 0.05) and mRS2 (r = 0.301, p < 0.05) in anti-NMDAR encephalitis patients. The receiver operating characteristic curve demonstrated that the combined detection of UA, HDL-C and CRP before treatment had a significantly higher value (the area under the curve = 0.848; 95% confidence interval [CI], 0.74–0.957; p < 0.001) to predict anti-NMDAR encephalitis accompanied with SE than that of single detection.

Conclusions

Hence, the combined detection of serum UA, HDL-C and CRP before treatment may be simple and effective indicators for predicting SE in anti-NMDAR encephalitis, which may be helpful in early stages to remind clinicians to be alert to the emergence of SE.
Women’s views on accepting COVID-19 vaccination during and after pregnancy, and for their babies: a multi-methods study in the UK
Authors: Skirrow Helen , Barnett Sara , Bell Sadie , Riaposova Lucia , Mounier-Jack Sandra , Kampmann Beate , Holder Beth
Published: January 2022

Background

COVID-19 vaccines are advised for pregnant women in the United Kingdom (UK) however COVID-19 vaccine uptake among pregnant women is inadequate.

Methods

An online survey and semi-structured interviews were used to investigate pregnant women’s views on COVID-19 vaccine acceptability for themselves when pregnant, not pregnant and for their babies. One thousand one hundred eighty-one women, aged over 16 years, who had been pregnant since 23rd March 2020, were surveyed between 3rd August–11th October 2020. Ten women were interviewed.

Results

The majority of women surveyed (81.2%) reported that they would ‘definitely’ or were ‘leaning towards’ accepting a COVID-19 vaccine when not pregnant. COVID-19 vaccine acceptance was significantly lower during pregnancy (62.1%, p < 0.005) and for their babies (69.9%, p < 0.005). Ethnic minority women were twice as likely to reject a COVID-19 vaccine for themselves when not pregnant, pregnant and for their babies compared to women from White ethnic groups (p < 0.005). Women from lower-income households, aged under 25-years, and from some geographic regions were more likely to reject a COVID-19 vaccine when not pregnant, pregnant and for their babies. Multivariate analysis revealed that income and ethnicity were the main drivers of the observed age and regional differences. Women unvaccinated against pertussis in pregnancy were over four times more likely to reject COVID-19 vaccines when not pregnant, pregnant and for their babies. Thematic analysis of the survey freetext responses and interviews found safety concerns about COVID-19 vaccines were common though wider mistrust in vaccines was also expressed. Trust in vaccines and the health system were also reasons women gave for accepting COVID-19 vaccines.

Conclusion

Safety information on COVID-19 vaccines must be clearly communicated to pregnant women to provide reassurance and facilitate informed pregnancy vaccine decisions. Targeted interventions to promote COVID-19 vaccine uptake among ethnic minority and lower-income women may be needed.
Memantine augmentation of sertraline in the treatment of symptoms and executive function among patients with obsessive-compulsive disorder: A double-blind placebo-controlled, randomized clinical trial
Authors: Askari Sanaz , Mokhtari Saba , Shariat Seyed Vahid , Shariati Behnam , Yarahmadi Masoomeh , Shalbafan Mohammadreza
Published: January 2022

Background

Medications currently recommended for the treatment of Obsessive-Compulsive Disorder (OCD) usually decrease the severity of the symptoms by 20–30%; however, 40–60% of OCD patients do not achieve a satisfactory response. Our main objective was to investigate the effectiveness of memantine, a non-competitive N-Methyl-D-aspartate (NMDA) receptor antagonist, as an adjunct therapy to sertraline, a selective serotonin reuptake inhibitor (SSRI), to improve severity of symptoms and executive function among patients with obsessive-compulsive disorder.

Methods

Seventy patients with OCD according to the Diagnostic and Statistical Manual of Mental Disorders (DSM–5) criteria, and a Yale-Brown obsessive compulsive scale (Y-BOCS) score of more than 21 were recruited to the study. They received sertraline (100 mg daily initially followed by 200 mg daily after week 4) and either memantine (10 mg twice daily) or placebo in a placebo controlled, double-blinded, parallel-group, clinical trial of 12 weeks. The primary outcome was OCD symptoms measured by the Y-BOCS. Moreover, executive function of participants was measured by the Wisconsin Card Sorting Test (WCST).

Results

The total score, and obsession and compulsion subscales of Y-BOCS significantly dropped in both groups with no significant difference between the two groups. However, memantine group showed a greater response in the number of completed categories subscale of the WCST (p value<0.001). We did not observe any major adverse effects in any of the groups.

Conclusion

Memantine has an acceptable safety and tolerability in patients with OCD and might have a positive effect on their executive function. Nevertheless, the current results don`t support the efficacy of memantine as an adjunctive agent to sertraline for symptoms in patients with OCD.

Trial registration

The trial was registered at the Iranian Registry of Clinical Trials on 04/10/2019 (www.irct.ir; IRCT ID: IRCT20170123032145N4).
Prevalence of risk behaviors and correlates of SARS-CoV-2 positivity among in-school contacts of confirmed cases in a Georgia school district in the pre-vaccine era, December 2020–January 2021
Authors: Hast Marisa , Swanson Megan , Scott Colleen , Oraka Emeka , Espinosa Catherine , Burnett Eleanor , Kukielka Esther A. , Rice Marion E. , Mehari Lemlem , McCloud Jazmyn , Miller Danielle , Franklin Rachel , Tate Jacqueline E. , Kirking Hannah L. , Morris Elana
Published: January 2022

Background

There is a continuing risk for COVID-19 transmission in school settings while transmission is ongoing in the community, particularly among unvaccinated populations. To ensure that schools continue to operate safely and to inform implementation of prevention strategies, it is imperative to gain better understanding of the risk behaviors of staff and students. This secondary analysis describes the prevalence of COVID-19 risk behaviors in an exposed population of students and school staff in the pre-vaccine era and identifies associations between these behaviors and testing positive for SARS-CoV-2.

Methods

From December 2020–January 2021, school staff and students exposed to confirmed COVID-19 cases in a Georgia school district were tested for SARS-CoV-2 and surveyed regarding risk behaviors in and out of school. Prevalence of risk behaviors was described by age group and school level, and associations with SARS-CoV-2 positivity were identified using chi squared tests.

Results

Overall, 717 students and 79 school staff participated in the investigation; SARS-CoV-2 positivity was 9.2%. In the 2 weeks prior to COVID-19 exposure, 24% of participants reported unmasked indoor time at school, 40% attended social gatherings with non-household members, and 71% visited out-of-school indoor locations, including 19% who ate indoors in restaurants. Frequencies of risk behaviors increased by age. Among students, 17% participated in school sports, of whom 86% participated without a mask. SARS-CoV-2 positivity was significantly associated with school sports and unmasked time in sports. Among K-5 students, positivity was associated with exposure to a teacher index case.

Conclusions

This analysis highlights the high prevalence of risk behaviors in an unvaccinated population exposed to COVID-19 in school and identifies an association between student sports participation and SARS-CoV-2 positivity. These findings illustrate the importance of school-level prevention measures to reduce SARS-CoV-2 transmission, including limiting close-contact indoor sports and promoting consistent mask use in unvaccinated individuals. Future research could explore the role of community vaccination programs as a strategy to reduce COVID-19 transmission and introductions into school settings.
The association between transfer coefficient of the lung and the risk of exacerbation in asthma-COPD overlap: an observational cohort study
Authors: Ogata Hiroaki , Katahira Katsuyuki , Enokizu-Ogawa Aimi , Jingushi Yujiro , Ishimatsu Akiko , Taguchi Kazuhito , Nogami Hiroko , Aso Hiroshi , Moriwaki Atsushi , Yoshida Makoto
Published: January 2022

Background

Asthma–chronic obstructive pulmonary disease (COPD) overlap (ACO) patients experience exacerbations more frequently than those with asthma or COPD alone. Since low diffusing capacity of the lung for carbon monoxide (DLCO) is known as a strong risk factor for severe exacerbation in COPD, DLCOor a transfer coefficient of the lung for carbon monoxide (KCO) is speculated to also be associated with the risk of exacerbations in ACO.

Methods

This study was conducted as an observational cohort survey at the National Hospital Organization Fukuoka National Hospital. DLCOand KCOwere measured in 94 patients aged ≥ 40 years with a confirmed diagnosis of ACO. Multivariable-adjusted hazard ratios (HRs) for the exacerbation-free rate over one year were estimated and compared across the levels of DLCOand KCO.

Results

Within one year, 33.3% of the cohort experienced exacerbations. After adjustment for potential confounders, low KCO(< 80% per predicted) was positively associated with the incidence of exacerbation (multivariable-adjusted HR = 3.71 (95% confidence interval 1.32–10.4)). The association between low DLCO(< 80% per predicted) and exacerbations showed similar trends, although it failed to reach statistical significance (multivariable-adjusted HR = 1.31 (95% confidence interval 0.55–3.11)).

Conclusions

Low KCOwas a significant risk factor for exacerbations among patients with ACO. Clinicians should be aware that ACO patients with impaired KCOare at increased risk of exacerbations and that careful management in such a population is mandatory.
Diabetic ketoacidosis (DKA) induced cerebral edema complicating small chronic subdural hematoma/hygroma/ at Zewuditu memorial hospital: a case report
Authors: Shiferaw Mestet Yibeltal , Laeke T/Mariam Tsegazeab , Aklilu Abenezer Tirsit , Akililu Yemisirach Bizuneh , Worku Bethelhem Yishak
Published: January 2022

Background

While both DKA & CSDH/subdural hygroma/ are known to cause significant morbidity and mortality, there is no a study that shows the role & effect of DKA on CSDH/subdural hygroma/ & vice versa to authors’ best knowledge; hence this work will show how important relation does exist between DKA & CSDH/ hygroma.

Case summary

This study highlights the diagnostic & management challenges seen for a case of a 44 years old female black Ethiopian woman admitted with a diagnosis of newly diagnosed type 1 DM with DKA + small CSDH/subdural hygroma/ after she presented with sever global headache and a 3 month history of lost to her work. She needed burrhole & evacuation for complete clinical improvement besides DKA’s medical treatment.

Conclusion

DKA induced cerebral edema on the CSDH/subdural hematoma/ can have a role in altering any of the parameters (except the thickness of CSDH) for surgical indication of patients with a diagnosis of both CSDH +DM with DKA. Hence, the treating physicians should be vigilant of different parameters that suggests tight brain &/ cerebral edema (including midline shift, the status of cisterns, fissures & sulci) and should not be deceived of the thickness of the CSDH/subdural hygroma/alone; especially when there is a disproportionately tight brain for the degree of collection. Whether DKA induced cerebral edema causes a subdural hygroma is unknown and needs further study.
Examining the views of operating room nurses and physicians on the relationship between professional values and professional communication
Authors: Yeganeh Sedigheh , Torabizadeh Camellia , Bahmani Tayebeh , Molazem Zahra , Doust Hamed Yeganeh , Dehnavi Samira Daneshvar
Published: January 2022

Purpose

Professional communication and professional values are two basic concepts in operating rooms and should be studied more closely in view of the nature of work and the high circulation of patients in operating rooms.

Methods

The present work is a descriptive-analytic study with a cross-sectional design. The sample was 603 operating room physicians and personnel selected from the public hospitals of Shiraz. The data collection instruments were the 41-item professional communication questionnaire and the 26-item professional values scale.

Results

The results showed that the operating room nurses and physicians perceived the status of professional communication and professional values to be satisfactory. As for professional communication, the participants’ perception of the domains of mutual respect and trust (p ≤ 0.001), teamwork (p ≤ 0.001), ethical competence (p ≤ 0.017), and workplace conflicts (p ≤ 0.001) was significant. As for professional values, only the dimension of care (p ≤ 0.016) was perceived to be significant. Moreover, a significant positive relationship was found to exist between professional communication and professional values (p ≤ 0.001).

Conclusion

Considering the significance of the concept of professional communication and its connection with professional values, it is recommended that operating room personnel and physicians receive systematic education about professional communication and the harms of destructive attitudes as part of their academic education and afterwards.
Exploring the experiences of nurses caring for patients with COVID-19: a qualitative study in Iran
Authors: Ahmadidarrehsima Sudabeh , Salari Nasibeh , Dastyar Neda , Rafati Foozieh
Published: January 2022

Background

The coronavirus disease (COVID-19) is now a major public health emergency in the world. Nurses as key members of the COVID-19 patient care team are exposed to most challenges caused by the disease. As exploring the experiences of nurses as patient supporters and caregivers can play an important role in improving the quality of care for patients with COVID-19 disease, the present study explored the experiences of nurses caring for patients with COVID-19.

Methods

The study employed a qualitative design. This study employed purposive sampling to select 10 nurses with bachelors and master’s degrees in nursing who were taking care of patients with COVID-19 in ICUs or inpatient wards in southern Iran. Data were collected through semi-structured interviews. The collected data were analyzed using the qualitative content analysis procedure proposed by Graneheim and Lundman.

Results

The analysis of the data revealed four main themes and ten sub-themes: A) physical, psychological, and social burden of care (excessive workload; fear, anxiety, worry; unpleasant social experiences; compassion fatigue) B) unmet needs (personal needs and professional needs) C) positive experiences (pleasant social experiences and inner satisfaction), and D) strategies (problem-solving strategies and stress symptom mitigation strategies).

Conclusions

An analysis of the themes and subthemes extracted in this study suggested that the nurses who participated in this study faced many personal and professional challenges. Therefore, health officials and specialists need to pay special attention to nurses’ challenges and needs.
Patients’ perspective on supposedly patient-relevant process and outcome parameters: a cross-sectional survey within the ‘PRO patients study’
Authors: Kersting Christine , Hülsmann Julia , Weckbecker Klaus , Mortsiefer Achim
Published: January 2022

Background

To be able to make informed choices based on their individual preferences, patients need to be adequately informed about treatment options and their potential outcomes. This implies that studies measure the effects of care based on parameters that are relevant to patients. In a previous scoping review, we found a wide variety of supposedly patient-relevant parameters that equally addressed processes and outcomes of care. We were unable to identify a consistent understanding of patient relevance and therefore aimed to develop an empirically based concept including a generic set of patient-relevant parameters. As a first step we evaluated the process and outcome parameters identified in the scoping review from the patients’ perspective.

Methods

We conducted a cross-sectional survey among German general practice patients. Ten research practices of Witten/Herdecke University supported the study. During a two-week period in the fall of 2020, patients willing to participate self-administered a short questionnaire. It evaluated the relevance of the 32 parameters identified in the scoping review on a 5-point Likert scale and offered a free-text field for additional parameters. These free-text answers were inductively categorized by two researchers. Quantitative data were analyzed using descriptive statistics. Bivariate analyses were performed to determine whether there are any correlations between rating a parameter as highly relevant and patients’ characteristics.

Results

Data from 299 patients were eligible for analysis. All outcomes except ‘sexuality’ and ‘frequency of healthcare service utilization’ were rated important. ‘Confidence in therapy’ was rated most important, followed by ‘prevention of comorbidity’ and ‘mobility’. Relevance ratings of five parameters were associated with patients’ age and gender, but not with their chronic status. The free-text analysis revealed 15 additional parameters, 12 of which addressed processes of care, i.e., ‘enough time in physician consultation’.

Conclusion

Patients attach great value to parameters addressing processes of care. It appears as though the way in which patients experience the care process is not less relevant than what comes of it. Relevance ratings were not associated with chronic status, but few parameters were gender- and age-related.

Trial registration

Core Outcome Measures in Effectiveness Trials Initiative, registration number: 1685.
Long-term in vivo application of a potassium channel-based optogenetic silencer in the healthy and epileptic mouse hippocampus
Authors: Kleis P. , Paschen E. , Häussler U. , Bernal Sierra Y. A. , Haas C. A.
Published: January 2022

Background

Optogenetic tools allow precise manipulation of neuronal activity via genetically encoded light-sensitive proteins. Currently available optogenetic inhibitors are not suitable for prolonged use due to short-lasting photocurrents, tissue heating, and unintended changes in ion distributions, which may interfere with normal neuron physiology. To overcome these limitations, a novel potassium channel-based optogenetic silencer, named PACK, was recently developed. The PACK tool has two components: a photoactivated adenylyl cyclase from Beggiatoa (bPAC) and a cAMP-dependent potassium channel, SthK, which carries a large, long-lasting potassium current in mammalian cells. Previously, it has been shown that activating the PACK silencer with short light pulses led to a significant reduction of neuronal firing in various in vitro and acute in vivo settings. Here, we examined the viability of performing long-term studies in vivo by looking at the inhibitory action and side effects of PACK and its components in healthy and epileptic adult male mice.

Results

We targeted hippocampal cornu ammonis (CA1) pyramidal cells using a viral vector and enabled illumination of these neurons via an implanted optic fiber. Local field potential (LFP) recordings from CA1 of freely moving mice revealed significantly reduced neuronal activity during 50-min intermittent (0.1 Hz) illumination, especially in the gamma frequency range. Adversely, PACK expression in healthy mice induced chronic astrogliosis, dispersion of pyramidal cells, and generalized seizures. These side effects were independent of the light application and were also present in mice expressing bPAC without the potassium channel. Light activation of bPAC alone increased neuronal activity, presumably via enhanced cAMP signaling. Furthermore, we applied bPAC and PACK in the contralateral hippocampus of chronically epileptic mice following a unilateral injection of intrahippocampal kainate. Unexpectedly, the expression of bPAC in the contralateral CA1 area was sufficient to prevent the spread of spontaneous epileptiform activity from the seizure focus to the contralateral hippocampus.

Conclusion

Our study highlights the PACK tool as a potent optogenetic inhibitor in vivo. However, further refinement of its light-sensitive domain is required to avoid unexpected physiological changes.
Use of an in silico knowledge discovery approach to determine mechanistic studies of silver nanoparticles-induced toxicity from in vitro to in vivo
Authors: Mao Bin-Hsu , Luo Yi-Kai , Wang Bour-Jr , Chen Chun-Wan , Cheng Fong-Yu , Lee Yu-Hsuan , Yan Shian-Jang , Wang Ying-Jan
Published: January 2022

Background

Silver nanoparticles (AgNPs) are considered a double-edged sword that demonstrates beneficial and harmful effects depending on their dimensions and surface coating types. However, mechanistic understanding of the size- and coating-dependent effects of AgNPs in vitro and in vivo remains elusive. We adopted an in silico decision tree-based knowledge-discovery-in-databases process to prioritize the factors affecting the toxic potential of AgNPs, which included exposure dose, cell type and AgNP type (i.e., size and surface coating), and exposure time. This approach also contributed to effective knowledge integration between cell-based phenomenological observations and in vitro/in vivo mechanistic explorations.

Results

The consolidated cell viability assessment results were used to create a tree model for generalizing cytotoxic behavior of the four AgNP types: SCS, LCS, SAS, and LAS. The model ranked the toxicity-related parameters in the following order of importance: exposure dose > cell type > particle size > exposure time ≥ surface coating. Mechanistically, larger AgNPs appeared to provoke greater levels of autophagy in vitro, which occurred during the earlier phase of both subcytotoxic and cytotoxic exposures. Furthermore, apoptosis rather than necrosis majorly accounted for compromised cell survival over the above dosage range. Intriguingly, exposure to non-cytotoxic doses of AgNPs induced G2/M cell cycle arrest and senescence instead. At the organismal level, SCS following a single intraperitoneal injection was found more toxic to BALB/c mice as compared to SAS. Both particles could be deposited in various target organs (e.g., spleen, liver, and kidneys). Morphological observation, along with serum biochemical and histological analyses, indicated that AgNPs could produce pancreatic toxicity, apart from leading to hepatic inflammation.

Conclusions

Our integrated in vitro, in silico, and in vivo study revealed that AgNPs exerted toxicity in dose-, cell/organ type- and particle type-dependent manners. More importantly, a single injection of lethal-dose AgNPs (i.e., SCS and SAS) could incur severe damage to pancreas and raise blood glucose levels at the early phase of exposure.
A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
Authors: Gingrich Ellen C. , Case Kendra , Garcia A. Denise R.
Published: January 2022

Background

The molecular signaling pathway, Sonic hedgehog (Shh), is critical for the proper development of the central nervous system. The requirement for Shh signaling in neuronal and oligodendrocyte development in the developing embryo are well established. However, Shh activity is found in discrete subpopulations of astrocytes in the postnatal and adult brain. Whether Shh signaling plays a role in astrocyte development is not well understood.

Methods

Here, we use a genetic inducible fate mapping approach to mark and follow a population of glial progenitor cells expressing the Shh target gene, Gli1, in the neonatal and postnatal brain.

Results

In the neonatal brain, Gli1-expressing cells are found in the dorsolateral corner of the subventricular zone (SVZ), a germinal zone harboring astrocyte progenitor cells. Our data show that these cells give rise to half of the cortical astrocyte population, demonstrating their substantial contribution to the cellular composition of the cortex. Further, these data suggest that the cortex harbors astrocytes from different lineages. Gli1 lineage astrocytes are distributed across all cortical layers, positioning them for broad influence over cortical circuits. Finally, we show that Shh activity recurs in mature astrocytes in a lineage-independent manner, suggesting cell-type dependent roles of the pathway in driving astrocyte development and function.

Conclusion

These data identify a novel role for Shh signaling in cortical astrocyte development and support a growing body of evidence pointing to astrocyte heterogeneity.
Masked diabetes insipidus in pituitary metastasis from breast cancer after thalamic biopsy: a case report
Authors: Hashimoto Hiroaki , Maruo Tomoyuki , Nakamura Masami , Ushio Yukitaka , Hirata Masayuki , Kishima Haruhiko
Published: January 2022

Background

Symptomatic pituitary metastasis is rare; furthermore, it can result in diabetes insipidus and panhypopituitarism. Since diabetes insipidus is masked by concurrent panhypopituitarism, it can impede the diagnosis of pituitary dysfunction.

Case presentation

A 68-year-old Japanese female suffering from pituitary and thalamic metastases caused by untreated breast cancer, underwent a biopsy targeting the thalamus, not the pituitary. She lacked prebiopsy pituitary dysfunction symptoms; however, these symptoms unexpectedly occurred after biopsy. Diabetes insipidus was masked by corticosteroid insufficiency, and she showed normal urinary output and plasma sodium levels. Upon commencement of glucocorticoid replacement therapy, the symptoms of diabetes insipidus appeared.

Conclusions

In this case, thalamic biopsy, as opposed to pituitary biopsy, was performed to preserve pituitary function. However, pituitary dysfunction could not be avoided. Caution is necessary for asymptomatic patients with pituitary metastases as invasive interventions, such as surgery, may induce pituitary dysfunction. Moreover, with respect to masked diabetes insipidus, there is a need to carefully consider pituitary dysfunction to avoid misdiagnosis and delayed treatment.
Secure machine learning against adversarial samples at test time
Authors: Lin Jing , Njilla Laurent L. , Xiong Kaiqi
Published: January 2022

Deep neural networks (DNNs) are widely used to handle many difficult tasks, such as image classification and malware detection, and achieve outstanding performance. However, recent studies on adversarial examples, which have maliciously undetectable perturbations added to their original samples that are indistinguishable by human eyes but mislead the machine learning approaches, show that machine learning models are vulnerable to security attacks. Though various adversarial retraining techniques have been developed in the past few years, none of them is scalable. In this paper, we propose a new iterative adversarial retraining approach to robustify the model and to reduce the effectiveness of adversarial inputs on DNN models. The proposed method retrains the model with both Gaussian noise augmentation and adversarial generation techniques for better generalization. Furthermore, the ensemble model is utilized during the testing phase in order to increase the robust test accuracy. The results from our extensive experiments demonstrate that the proposed approach increases the robustness of the DNN model against various adversarial attacks, specifically, fast gradient sign attack, Carlini and Wagner (C&W) attack, Projected Gradient Descent (PGD) attack, and DeepFool attack. To be precise, the robust classifier obtained by our proposed approach can maintain a performance accuracy of 99% on average on the standard test set. Moreover, we empirically evaluate the runtime of two of the most effective adversarial attacks, i.e., C&W attack and BIM attack, to find that the C&W attack can utilize GPU for faster adversarial example generation than the BIM attack can. For this reason, we further develop a parallel implementation of the proposed approach. This parallel implementation makes the proposed approach scalable for large datasets and complex models.
Existence and asymptotic properties of singular solutions of nonlinear elliptic equations in $R^{n}\backslash\{0\}$
Authors: Bachar Imed , Aljarallah Entesar
Published: January 2022

We consider the following singular semilinear problem$$ \textstyle\begin{cases} \Delta u(x)+p(x)u^{\gamma }=0,\quad x\in D ~(\text{in the distributional sense}), \\ u>0,\quad \text{in }D, \\ \lim_{ \vert x \vert \rightarrow 0} \vert x \vert ^{n-2}u(x)=0, \\ \lim_{ \vert x \vert \rightarrow \infty }u(x)=0,\end{cases} $${Δu(x)+p(x)uγ=0,x∈D(in the distributional sense),u>0,in D,lim|x|→0|x|n−2u(x)=0,lim|x|→∞u(x)=0,where$\gamma <1$γ<1,$D=\mathbb{R}^{n}\backslash \{0\}$D=Rn∖{0}($n\geq 3$n≥3) andpis a positive continuous function inD, which may be singular at$x=0$x=0. Under sufficient conditions for the weighted function$p(x)$p(x), we prove the existence of a positive continuous solution onD, which could blow-up at the origin. The global asymptotic behavior of this solution is also obtained.
Stochastic optimal control with random coefficients and associated stochastic Hamilton–Jacobi–Bellman equations
Authors: Moon Jun
Published: January 2022

We consider the optimal control problem for stochastic differential equations (SDEs) with random coefficients under the recursive-type objective functional captured by the backward SDE (BSDE). Due to the random coefficients, the associated Hamilton–Jacobi–Bellman (HJB) equation is a class of second-order stochastic PDEs (SPDEs) driven by Brownian motion, which we call the stochastic HJB (SHJB) equation. In addition, as we adopt the recursive-type objective functional, the drift term of the SHJB equation depends on the second component of its solution. These two generalizations cause several technical intricacies, which do not appear in the existing literature. We prove the dynamic programming principle (DPP) for the value function, for which unlike the existing literature we have to use the backward semigroup associated with the recursive-type objective functional. By the DPP, we are able to show the continuity of the value function. Using the Itô–Kunita’s formula, we prove the verification theorem, which constitutes a sufficient condition for optimality and characterizes the value function, provided that the smooth (classical) solution of the SHJB equation exists. In general, the smooth solution of the SHJB equation may not exist. Hence, we study the existence and uniqueness of the solution to the SHJB equation under two different weak solution concepts. First, we show, under appropriate assumptions, the existence and uniqueness of the weak solution via the Sobolev space technique, which requires converting the SHJB equation to a class of backward stochastic evolution equations. The second result is obtained under the notion of viscosity solutions, which is an extension of the classical one to the case for SPDEs. Using the DPP and the estimates of BSDEs, we prove that the value function is the viscosity solution to the SHJB equation. For applications, we consider the linear-quadratic problem, the utility maximization problem, and the European option pricing problem. Specifically, different from the existing literature, each problem is formulated by the generalized recursive-type objective functional and is subject to random coefficients. By applying the theoretical results of this paper, we obtain the explicit optimal solution for each problem in terms of the solution of the corresponding SHJB equation.
Experiment and Analysis of Mechanical Properties of Lightweight Concrete Prefabricated Building Structure Beams
Authors: Fang Yingguang , Xu Yafei , Gu Renguo
Published: January 2022

Recent years have witnessed that the prefabricated concrete structure is in the widespread use of building structures. This structure, however, still has some weaknesses, such as excessive weight of components, high requirements for construction equipment, difficult alignment of nodes, and poor installation accuracy. In order to handle the problems mentioned above, the prefabricated component made of lightweight concrete is adopted. At the same time, this prefabricated component is beneficial to reducing the load of the building structure itself and improving the safety and economy of the building structure. Nevertheless, it is rarely found that the researches and applications of lightweight concrete for stressed members are conducted. In this context, this paper replaces ordinary coarse aggregate with lightweight ceramsite or foam based on the C60 concrete mix ratio so as to obtain a mix ratio of C40 lightweight concrete that meets the engineering standards. Besides, ceramsite concrete beams and foamed concrete beams are fabricated. Moreover, through three-point bending tests, this paper further explores the mechanical properties of lightweight concrete beams and plain concrete beams during normal use conditions. As demonstrated in the results, the mechanical properties of the foamed concrete beam are similar to those of the plain concrete beam. Compared to plain concrete beams, the density of foamed concrete beams was lower by 23.4%; moreover, the ductility and toughness of foamed concrete were higher by 13% and 3%, respectively. However, in comparison with the plain concrete beam, the mechanical properties of the ceramsite concrete beam have some differences, with relatively large dispersion and obvious brittle failure characteristics. Moreover, in consideration of the nonlinear deformation characteristics of reinforced concrete beams, the theoretical calculation value of beam deflection was given in this paper based on the assumption of flat section and the principle of virtual work. The theoretically calculated deflection values of ordinary concrete beams and foamed concrete beams are in good agreement with the experimental values under normal use conditions, verifying the rationality and effectiveness of the calculation method. The research results of this paper can be taken as a reference for similar engineering designs.
Cytogenetic effect of some nanostructure polymers prepared via gamma irradiation on Vicia faba plant
Authors: Salah M. , Yehia Soad , Ali Rania T.
Published: January 2022

Background

Nourishment plants during the field time is a must; to have healthy, high productive and self-propagating plants. The trendy nano-fertilizers came to the front in modernized agriculture seeking for minimizing the soil suffocation with other chemical fertilizers in the bulk size. Nano-fertilizers may represent a way out of shot as they are completely absorbed by plant due to their small size, also it magnifies the benefit to the plant due to its high surface area. Nano-fertilizers are introduced via different way of synthesis methods. In this work, three of new nanocomposites are prepared in nano form via Gamma irradiation from Cobalt 60 source at irradiation dose 5 KGy. These composites which can supply plants with P, Zn elements needs to be revised for their safety usage in agriculture.

Methodology

Three compounds; Zinc oxide, phosphorous and the mixed Zinc–phosphorous elements were prepared in nano-composite forms coated with PVP as a shell and then characterized by HR-TEM, UV and FT-IR to emphasize their new sizes and shapes, then, they were examined for their cytotoxicity in three concentrations (0.5, 1 and 2%) on Vicia faba plants; after 3 h of direct roots treatment. Cytotoxicity test concerned the mitotic index, phase index, abnormal mitosis and the type of the aberrations at each phase.

Results

The three tested NPs exerted mito-accelerating effect on root meristematic cells. However, concentration‐dependent genotoxicity was also an evident.

Conclusion

The three examined nano-composites may recommend to be used in the lowest examined concentrations to minimize its harm effect on the plant cell and keep their benefits to the environment. It also recommended to count the Zn/P mix NPs over ZN or P separately as it induces an intermediating cytogenetic effect on mitosis apparatus of Vicia faba plant.

Graphical Abstract
In-Competition Severe Injury Events in Elite Alpine Ski Racing from 1997 to 2020: The Case of the Austrian Ski Team
Authors: Barth Michael , Platzer Hans-Peter , Forstinger Carina Andrea , Innerhofer Gunnar , Giger Anton , Schröcksnadel Peter , Nachbauer Werner
Published: January 2022

Background

To increase safety in elite alpine ski racing Injury Surveillance Systems were implemented and preventive measures introduced. However, studies analysing the change in athletes’ injury risk by controlling for their exposure are still scarce.

Objectives

This study aimed to describe and analyse the risk of in-competition severe injury events (SIEcomp) in elite alpine ski racing.

Methods

Data recorded in the Austrian Ski Federation’s Injury Surveillance System were used to analyse the SIEcomp incidence. Information on athletes’ competition exposure was obtained from the official website of the International Ski Federation. In 23 seasons, 2333 skier seasons were recorded for the Austrian Ski Team. Within a total of 114,531 runs 169 SIEcomp occurred. Generalised Estimating Equation for Poisson Regressions were applied.

Results

The SIEcomp incidence per 1000 runs was 1.48 [95% confidence interval (CI) 1.26–1.73] for elite alpine ski racers and 2.21 (95% CI 1.79–2.75) for the subgroup of World Cup racers. A significant sex difference was detected for the subgroup of junior racers with a higher risk for female athletes [risk ratio (RR): 2.97, 95% CI 1.46–6.05]. Between the seasons of 1997 and 2020, the seasonal SIEcomp incidence increased by a factor of 2.67 for elite alpine ski racers and 3.53 for World Cup racers. Downhill (2.75, 95% CI 2.18–3.47) had the highest SIEcomp incidence, followed by super-G (1.94, 95% CI 1.30–2.88), giant slalom (1.40, 95% CI 1.06–1.85), and slalom (0.64, 95% CI 0.43–0.96).

Conclusion

Although many preventive measures have been implemented in elite alpine ski racing, the risk of SIEcomp has increased over the last two decades.
Causal theory error in college students’ understanding of science studies
Authors: Seifert Colleen M. , Harrington Michael , Michal Audrey L. , Shah Priti
Published: January 2022

When reasoning about science studies, people often make causal theory errors by inferring or accepting a causal claim based on correlational evidence. While humans naturally think in terms of causal relationships, reasoning about science findings requires understanding how evidence supports—or fails to support—a causal claim. This study investigated college students’ thinking about causal claims presented in brief media reports describing behavioral science findings. How do science students reason about causal claims from correlational evidence? And can their reasoning be improved through instruction clarifying the nature of causal theory error? We examined these questions through a series of written reasoning exercises given to advanced college students over three weeks within a psychology methods course. In a pretest session, students critiqued study quality and support for a causal claim from a brief media report  suggesting an association between two variables. Then, they created diagrams depicting possible alternative causal theories. At the beginning of the second session, an instructional intervention introduced students to an extended example of a causal theory error through guided questions about possible alternative causes. Then, they completed the same two tasks with new science reports immediately and again 1 week later. The results show students’ reasoning included fewer causal theory errors after the intervention, and this improvement was maintained a week later. Our findings suggest that interventions aimed at addressing reasoning about causal claims in correlational studies are needed even for advanced science students, and that training on considering alternative causal theories may be successful in reducing casual theory error.
Assessment of four strain energy decomposition methods for phase field fracture models using quasi-static and dynamic benchmark cases
Authors: Zhang Shuaifang , Jiang Wen , Tonks Michael R.
Published: January 2022

Strain energy decomposition methods in phase field fracture models separate strain energy that contributes to fracture from that which does not. However, various decomposition methods have been proposed in the literature, and it can be difficult to determine an appropriate method for a given problem. The goal of this work is to facilitate the choice of strain decomposition method by assessing the performance of three existing methods (spectral decomposition of the stress or the strain and deviatoric decomposition of the strain) and one new method (deviatoric decomposition of the stress) with several benchmark problems. In each benchmark problem, we compare the performance of the four methods using both qualitative and quantitative metrics. In the first benchmark, we compare the predicted mechanical behavior of cracked material. We then use four quasi-static benchmark cases: a single edge notched tension test, a single edge notched shear test, a three-point bending test, and a L-shaped panel test. Finally, we use two dynamic benchmark cases: a dynamic tensile fracture test and a dynamic shear fracture test. All four methods perform well in tension, the two spectral methods perform better in compression and with mixed mode (though the stress spectral method performs the best), and all the methods show minor issues in at least one of the shear cases. In general, whether the strain or the stress is decomposed does not have a significant impact on the predicted behavior.