bluuebunny/crossref_metadata_2025 huggingface dataset:
Saponin-Rich Plant Premixture Supplementation Is as Efficient as Ionophore Monensin Supplementation Under Experimental Eimeria spp Challenge in Broiler Chicken
Authors: Benarbia Mohammed el Amine , Gaignon Pierre , Manoli Claire , Chicoteau Pierre
Published: July 2022

For decades avian coccidiosis prevention was based on the use of synthetic coccidiostats. However, their intensive use led to the development of resistance phenomena. In addition, societal demand is increasing for antibiotic-free animal products. Thus, there is a need for a natural and efficient solution for coccidiosis management. Saponin-rich plants, like Yucca schidigera and Trigonella foenum-graecum, are promising tools for coccidiosis management. This study assessed the effects of supplementing broiler chickens with a commercial blend of these two plants (NorponinXO2) under an experimental Eimeria challenge and compared their effects to monensin supplementation. Three trials were performed. For each trial, chickens were divided into 4 groups, untreated uninfested control (UUC), infested untreated control (IUC), infested supplemented with 120 ppm of Monensin in feed (PM), and infested supplemented with 250 ppm of Norponin XO2 in the feed (PX). Chickens were raised in cages; experimental infestation was performed on d14. On d21, intestinal lesions (ILs) scores and growth performances were recorded. A statistical study was carried out on each trial, as well as data from the 3 trials. Experimental infestation reduced in a significant way final body weight in IUC broilers compared to UUC broilers. This loss was numerically compensated by PM and PX treatment. As expected, intestinal lesions were almost absent in the UUC group; however, broilers from the IUC group showed a higher intestinal lesion occurrence. Supplementations with Monensin and NPXO were able to reduce intestinal lesions occurrence. These results suggest that NPXO supplementation is as efficient as Monensin in managing coccidiosis.
Nectar Secretion, Morphology, Anatomy and Ultrastructure of Floral Nectary in Selected Rubus idaeus L. Varieties
Authors: Kostryco Mikołaj , Chwil Mirosława
Published: July 2022

The distinctive features of floral nectaries facilitate identification of ecological and phylogenetic links between related taxa. The structure and functioning of nectaries determine the relationships between plants, pollinators, and the environment. The aim of the study was to determine and compare the micromorphology of the epidermis in the floral nectaries of six Rubus idaeus cultivars belonging to biennial (‘Glen Ample’, ‘Laszka’, ‘Radziejowa’) and repeated fruiting (‘Pokusa’, ‘Polana’, ‘Polka’) groups. Another objective was to characterize the cuticle ornamentation and stomatal morphology, the anatomy of the nectary epidermis, parenchyma, and sub-nectary parenchyma in the initial nectar secretion phase, as well as the ultrastructure of the nectary epidermis and parenchyma cells in the initial and full nectar secretion phases. The study was carried out using light, fluorescence, scanning and transmission-electron microscopy techniques. Semi-thin and ultrathin sections were used for the microscopic analyses. The cuticular ornamentation and stomatal morphology may be helpful elements in the identification of relatedness between Rubus species. The interaction of the extensive system of endoplasmic reticulum membranes, mitochondria, and Golgi apparatus indicates high metabolic activity, and the fusion of transport vesicles with the membrane suggests granulocrine nectar secretion. The results bring new data to the biology of plants.
Online Teaching Alternative in Human Anatomy
Authors: Garcia Barrios Alberto , Cisneros Gimeno Ana Isabel , Garza García María Camen , Lamiquiz Moneo Itziar , Whyte Orozco Jaime
Published: July 2022

The objective was to implement a “Breakout” activity using an online platform (Genially®) similar to those carried out in person to increase interactivity, motivation, and teamwork. The activity was proposed during the 2021–2022 academic year in the Human Anatomy II (Splanchnology) course taken in the second semester of the Bachelor’s Degree in Medicine (University of Zaragoza, Zaragoza, Spain) and was carried out with the participation of 89 students enrolled in the course. The evaluation of the experience by the students was carried out by means of an online questionnaire that comprised four questions (based on the Likert scale) and by the teaching staff through a coordination meeting. In total, 86% of the students agreed regarding the positive effect of these kinds of activities on interactivity and motivation, with 65% agreeing on the usefulness of these tools. Around 70% agree that this activity helped them to integrate course content and to enhance teamwork.
A Grant-Free Random Access Process for Low-End Distribution System Using Deep Neural Network
Authors: Almahjoub Alhusein , Qiu Dongyu
Published: July 2022

With the rising number of Internet of Things (IoT) devices joining the communication network, data exchange is increased tremendously resulting in network congestion. This paper deals with the optimal transmission of IoT devices to maximize the chances of success in random access procedures. With every machine trying to use the network for the transfer of data, IoT devices pose serious challenges to the already deployed infrastructure network. With a huge number of IoT devices and fixed limited resources, the existing handshaking-based random access process is not effective. To address this research gap, we propose a grant-free procedure while considering orthogonal transmission and devise a strategy to minimize collisions and idle events and maximize success. We use deep neural networks (DNN) that take channel conditions as an input to predict the device’s transmission for a successful maximization. In order to evaluate the performance of our proposed algorithm, we calculated the average delay with respect to channel coefficient and arrival rate in addition to the number of successes against the channel coefficient. Simulation results show that the proposed algorithm performs well and conforms with the claim of a successful maximization.
Melanoma Stem Cells Educate Neutrophils to Support Cancer Progression
Authors: Anselmi Martina , Fontana Fabrizio , Marzagalli Monica , Gagliano Nicoletta , Sommariva Michele , Limonta Patrizia
Published: July 2022

Background: It is now well-established that cancer stem cells (CSCs) can support melanoma progression by reshaping the tumor immune microenvironment. However, the molecular mechanisms underlying the crosstalk between melanoma SCs and cancer-associated neutrophils have not been elucidated yet. Methods: The aim of the present study was to unravel the role of melanoma SCs in neutrophil polarization. HL60 neutrophil-like (dHL60) cells were treated with conditioned medium from A375 melanoma SCs (CSC-CM), and their phenotype was investigated. Results: We demonstrated that CSC-CM could specifically activate immune cells by increasing CD66b and CD11b expression. In particular, we revealed that A375 CSCs could release various soluble factors, namely TGF-β, IL-6, and IL-8, able to promote the recruitment of neutrophils and their switch toward an N2 phenotype characterized by the activation of ERK, STAT3, and P38 pathways and the overexpression of CXCR2 and NF-kB. Moreover, after exposure to CSC-CM, dHL60 cells exhibited enhanced ROS production and NET release, without undergoing cell death; increased secretion of MMP-9 and pro-inflammatory cytokines was also observed. Finally, CSC-CM-activated neutrophils endowed A375 cells with stemness traits, stimulating both sphere formation and ABCG2 expression. Conclusion: Collectively, our results suggest that melanoma SCs can prime neutrophils to support cancer progression.
Diffusion Weighted Imaging in Gliomas: A Histogram-Based Approach for Tumor Characterization
Authors: Gihr Georg , Horvath-Rizea Diana , Kohlhof-Meinecke Patricia , Ganslandt Oliver , Henkes Hans , Härtig Wolfgang , Donitza Aneta , Skalej Martin , Schob Stefan
Published: July 2022

(1) Background: Astrocytic gliomas present overlapping appearances in conventional MRI. Supplementary techniques are necessary to improve preoperative diagnostics. Quantitative DWI via the computation of apparent diffusion coefficient (ADC) histograms has proven valuable for tumor characterization and prognosis in this regard. Thus, this study aimed to investigate (I) the potential of ADC histogram analysis (HA) for distinguishing low-grade gliomas (LGG) and high-grade gliomas (HGG) and (II) whether those parameters are associated with Ki-67 immunolabelling, the isocitrate-dehydrogenase-1 (IDH1) mutation profile and the methylguanine-DNA-methyl-transferase (MGMT) promoter methylation profile; (2) Methods: The ADC-histograms of 82 gliomas were computed. Statistical analysis was performed to elucidate associations between histogram features and WHO grade, Ki-67 immunolabelling, IDH1 and MGMT profile; (3) Results: Minimum, lower percentiles (10th and 25th), median, modus and entropy of the ADC histogram were significantly lower in HGG. Significant differences between IDH1-mutated and IDH1-wildtype gliomas were revealed for maximum, lower percentiles, modus, standard deviation (SD), entropy and skewness. No differences were found concerning the MGMT status. Significant correlations with Ki-67 immunolabelling were demonstrated for minimum, maximum, lower percentiles, median, modus, SD and skewness; (4) Conclusions: ADC HA facilitates non-invasive prediction of the WHO grade, tumor-proliferation rate and clinically significant mutations in case of astrocytic gliomas.
Long-Term Treatment with Bortezomib Induces Specific Methylation Changes in Differentiated Neuronal Cells
Authors: Łuczkowska Karolina , Taryma-Leśniak Olga , Bińkowski Jan , Sokołowska Katarzyna E. , Strapagiel Dominik , Jarczak Justyna , Paczkowska Edyta , Machaliński Bogusław , Wojdacz Tomasz K.
Published: July 2022

Bortezomib (BTZ) is proteasome inhibitor, effectively used in the treatment of multiple myeloma, but frequently discontinued due to peripheral neuropathy, which develops in patients after consecutive treatment cycles. The molecular mechanisms affected by BTZ in neuronal cells, which result in neuropathy, remain unknown. However, BTZ is unlikely to lead to permanent morphological nerve damage, because neuropathy reverses after discontinuation of treatment, and nerve cells have very limited renewal capacity. We have previously shown that BTZ induces methylation changes in SH-SY5Y cells, which take part in the development of treatment resistance. Here, we hypothesized that BTZ affects the methylomes of mature neurons, and these changes are associated with BTZ neurotoxicity. Thus, we studied methylomes of neuronal cells, differentiated from the LUHMES cell line, after cycles of treatment with BTZ. Our results show that BTZ induces specific methylation changes in mature neurons, which are not present in SH-SY5Y cells after BTZ treatment. These changes appear to affect genes involved in morphogenesis, neurogenesis, and neurotransmission. Furthermore, identified methylation changes are significantly enriched within binding sites of transcription factors previously linked to neuron physiology, including EBF, PAX, DLX, LHX, and HNF family members. Altogether, our results indicate that methylation changes are likely to be involved in BTZ neurotoxicity.
Treatment of RET-Positive Advanced Medullary Thyroid Cancer with Multi-Tyrosine Kinase Inhibitors—A Retrospective Multi-Center Registry Analysis
Authors: Koehler Viktoria Florentine , Adam Pia , Fuss Carmina Teresa , Jiang Linmiao , Berg Elke , Frank-Raue Karin , Raue Friedhelm , Hoster Eva , Knösel Thomas , Schildhaus Hans-Ulrich , Negele Thomas , Siebolts Udo , Lorenz Kerstin , Allelein Stephanie , Schott Matthias , Spitzweg Christine , Kroiss Matthias
Published: July 2022

Background: RET (rearranged during transfection) variants are the most prevalent oncogenic events in medullary thyroid cancer (MTC). In advanced disease, multi-tyrosine kinase inhibitors (MKIs) cabozantinib and vandetanib are the approved standard treatment irrespective of RET status. The actual outcome of patients with RET-positive MTC treated with MKIs is ill described. Methods: We here retrospectively determined the RET oncogene variant status with a targeted DNA Custom Panel in a prospectively collected cohort of 48 patients with advanced MTC treated with vandetanib and/or cabozantinib at four German referral centers. Progression-free survival (PFS) and overall survival (OS) probabilities were estimated using the Kaplan-Meier method. Results: In total, 44/48 (92%) patients had germline or somatic RET variants. The M918T variant was found in 29/44 (66%) cases. In total, 2/32 (6%) patients with a somatic RET variant had further somatic variants, while in 1/32 (3%) patient with a germline RET variant, additional variants were found. Only 1/48 (2%) patient had a pathogenic HRAS variant, and no variants were found in 3 cases. In first-line treatment, the median OS was 53 (95% CI (95% confidence interval), 32–NR (not reached); n = 36), and the median PFS was 21 months (12–39; n = 33) in RET-positive MTC patients. In second-line treatment, the median OS was 18 (13–79; n = 22), and the median PFS was 3.5 months (2–14; n = 22) in RET-positive cases. Conclusions: RET variants were highly prevalent in patients with advanced MTC. The treatment results in RET-positive cases were similar to those reported in unselected cohorts.
Cold Tolerance in Pinewood Nematode Bursaphelenchus xylophilus Promoted Multiple Invasion Events in Mid-Temperate Zone of China
Authors: Li Zhenxiao , Tao Jing , Zong Shixiang
Published: July 2022

Pinewood nematode (Bursaphelenchus xylophilus) is a highly destructive invasive species, causing extensive economic and ecological losses across Eurasia. It has recently invaded mid-temperate zone of northern China, threating large areas of coniferous forests. Herein, we evaluated the physiological and molecular basis of cold tolerance in pinewood nematode isolates from different temperature zones of China. After exposure to −5 °C and −10 °C, the survival rates of five pinewood nematode isolates from different temperature zones were 93.94%–94.77% and 43.26%–45.58% after 8 h, and 93.04%–94.85% and 9.93%–10.56% after 24 h, without significant differences among isolates. In a comparison of an isolate from a mid-temperate zone and an isolate from a subtropical zone under gradient cooling, the survival rates remained steady at nearly 95% when minimum temperature ranged from −5 °C to −15 °C, with no significant difference between isolates. In addition, phylogenetic and population structure analyses based on whole genome resequencing data suggested that isolates from mid-temperate and warm temperate zones are clustered with different isolates from subtropical zone, with no obvious geographic pattern. We did not detect significant variation in cold tolerance ability and selected gene among pinewood nematode isolates from different temperature zones. The recently invaded pinewood nematodes in the mid-temperate zone of northern China may spread by multiple invasion events from southern China, without adaptive revolution. Our research implies that it is important to reinforce quarantine inspection to control the rapid spread of pinewood nematode.
Xylem Transcriptome Analysis in Contrasting Wood Phenotypes of Eucalyptus urophylla × tereticornis Hybrids
Authors: Zhu Xianliang , He Jiayue , Zhou Changpin , Weng Qijie , Chen Shengkan , Bush David , Li Fagen
Published: July 2022

An investigation of the effects of two important post-transcriptional regulatory mechanisms, gene transcription and alternative splicing (AS), on the wood formation of Eucalyptusurophylla × tereticornis, an economic tree species widely planted in southern China, was carried out. We performed RNA-seq on E. urophylla × tereticornis hybrids with highly contrasting wood basic density (BD), cellulose content (CC), hemicellulose content (HC), and lignin content (LC). Signals of strong differentially expressed genes (DEGs) and differentially spliced genes (DSGs) were detected in all four groups of wood properties, suggesting that gene transcription and selective splicing may have important regulatory roles in wood properties. We found that there was little overlap between DEGs and DSGs in groups of the same trait. Furthermore, the key DEGs and DSGs that were detected simultaneously in the four groups tended to be enriched in different Gene Ontology terms, Kyoto Encyclopedia of Genes and Genomes pathways, and transcription factors. These results implied that regulation of gene transcription and AS is controlled by independent regulatory systems in wood formation. Lastly, we detected transcript levels of known wood biosynthetic genes and found that 79 genes encoding mainly enzymes or proteins such as UGT, LAC, CAD, and CESA may be involved in the positive or negative regulation of wood properties. This study reveals potential molecular mechanisms that may regulate wood formation and will contribute to the genetic improvement of Eucalyptus.
Non-Pharmacological Interventions for the Management of Chronic Health Conditions and Non-Communicable Diseases
Authors: Castellano-Tejedor Carmina
Published: July 2022

A chronic health condition has been defined by the World Health Organization (WHO) as a disease being of long duration, generally slow in progression and not passed from person to person; that is to say, a non-communicable disease (NCD) [...]
Valuation of Ecosystem Services for the Sustainable Development of Hani Terraces: A Rice–Fish–Duck Integrated Farming Model
Authors: Yuan Yuan , Xu Gangchun , Shen Nannan , Nie Zhijuan , Li Hongxia , Zhang Lin , Gong Yunchong , He Yanhui , Ma Xiaofei , Zhang Hongyan , Zhu Jian , Duan Jinrong , Xu Pao
Published: July 2022

As a complementary and symbiotic agro-ecological cycle system, a nature-based integrated rice–fish–duck farming ecosystem was developed in the Honghe Hani Rice Terraces. The main research objective was to evaluate the ecosystem services based on case studies of the Hani integrated rice–fish–duck terraced farming system and determine its potential and its importance as an ecological asset. We developed a valuation model to assess the value of the integrated farming system based on the three aspects of provisioning, regulation and maintenance, and cultural services; we selected eight groups and 10 indictors to evaluate the ecosystem services of the integrated ecosystem in Honghe Hani Rice Terraces was 3.316 billion CNY, of which the provisioning service value was 1.76 billion CNY, the regulation and maintenance service value was 1.32 billion CNY, and the cultural services value was 230.85 million CNY. The evaluation will be useful as a theoretical reference for poverty alleviation policy makers in similar poverty-stricken areas, enabling them to better protect and promote this mode of farming and further promote the protection of the natural environment and cultural heritage alongside the sustainable development of natural resources and human well-being.
Development of a Quantitative Preference Instrument for Person-Centered Dementia Care—Stage 2: Insights from a Formative Qualitative Study to Design and Pretest a Dementia-Friendly Analytic Hierarchy Process Survey
Authors: Mohr Wiebke , Rädke Anika , Afi Adel , Mühlichen Franka , Platen Moritz , Scharf Annelie , Michalowsky Bernhard , Hoffmann Wolfgang
Published: July 2022

Person-centered care (PCC) requires knowledge about patient preferences. An analytic hierarchy process (AHP) is one approach to quantify, weigh and rank patient preferences suitable for People living with Dementia (PlwD), due to simple pairwise comparisons of individual criteria from a complex decision problem. The objective of the present study was to design and pretest a dementia-friendly AHP survey. Methods: Two expert panels consisting of n = 4 Dementia Care Managers and n = 4 physicians to ensure content-validity, and “thinking-aloud” interviews with n = 11 PlwD and n = 3 family caregivers to ensure the face validity of the AHP survey. Following a semi-structured interview guide, PlwD were asked to assess appropriateness and comprehensibility. Data, field notes and partial interview transcripts were analyzed with a constant comparative approach, and feedback was incorporated continuously until PlwD had no further comments or struggles with survey completion. Consistency ratios (CRs) were calculated with Microsoft® Excel and ExpertChoice Comparion®. Results: Three main categories with sub-categories emerged: (1) Content: clear task introduction, (sub)criteria description, criteria homogeneity, (sub)criteria appropriateness, retest questions and sociodemography for heterogeneity; (2) Format: survey structure, pairwise comparison sequence, survey length, graphical design (incl. AHP scale), survey procedure explanation, survey assistance and response perspective; and (3) Layout: easy wording, short sentences and visual aids. Individual CRs ranged from 0.08 to 0.859, and the consolidated CR was 0.37 (0.038). Conclusions: Our formative qualitative study provides initial data for the design of a dementia-friendly AHP survey. Consideration of our findings may contribute to face and content validity in future quantitative preference research in dementia.
Public Opinion on Food Policies to Combat Obesity in Spain
Authors: Cavero Esponera Cristina , Fernández Sánchez-Escalonilla Sara , Royo-Bordonada Miguel Ángel
Published: July 2022

(1) Introduction: Poor diet is the fourth-leading cause of mortality in Spain, where adherence to the Mediterranean diet has declined in recent decades. To remedy this situation, a series of food policies have been proposed that would be easier to implement if they had public support. (2) Material and methods: Cross-sectional study covering a representative sample of the Spanish population (n = 1002), using telephone interviews that addressed nine food policies under four headings, namely, advertising, labeling, composition, and provision and sale. The sociodemographic determinants of support for these policies were analyzed using the chi-squared (χ2) test and Poisson multiple regression models with robust variance. (3) Results: All the proposed measures received more than 60% support. The policies that received greatest support were those targeting labeling at 96.6%, while the policies that received the least support were those directed at banning free refills at restaurants, at 63%. Support for policies was higher among women, older adults, and persons professing left-wing ideological affiliations. Compared with men, women’s support for advertising policies was 21% higher: similarly, compared with the youngest age group (18–29 years), support by the over-65 segment for provision and sale policies was 52% higher. Support for composition policies was 28% lower among persons with right-wing as opposed to left-wing political sympathies. (4) Conclusions: The authorities enjoy the support of the Spanish public as regards implementing food policies proposed by experts and overcoming the resistance of sectors opposed to such measures.
Italian Validation of the 12-Item Version of the Burnout Assessment Tool (BAT-12)
Authors: Mazzetti Greta , Consiglio Chiara , Santarpia Ferdinando Paolo , Borgogni Laura , Guglielmi Dina , Schaufeli Wilmar B.
Published: July 2022

The Burnout Assessment Tool (BAT) has shown satisfactory validity evidence in several countries, with the 23-item version of the instrument reporting adequate psychometric properties also in the Italian context. This paper is aimed to present results from the Italian validation of the 12-item version of the BAT. Based on a sample of 2277 workers, our results supported the factorial validity of a higher-order model represented by 4 first-order factors corresponding to the core dimensions of burnout, namely exhaustion, mental distance, and emotional and cognitive impairment. The measure invariance of the BAT-12 between data collected before and during the COVID-19 pandemic was supported. However, ANCOVA results suggest a higher score on the second-order burnout factor on data collected during the COVID-19 pandemic in comparison with earlier data. In line with the JD-R model, the BAT-12 total score reported a positive association with job demands (i.e., workload, time pressure, and role conflict) and a negative association with job resources (i.e., job autonomy, coworkers’ support) and personal resources (i.e., optimism, social self-efficacy, and task self-efficacy). Additionally, the BAT-12 showed a negative association with work engagement components (i.e., vigor, dedication, and absorption) and positive job attitudes (i.e., job satisfaction, affective commitment). All in all, our results identify the Italian version of the BAT-12 as a brief and reliable tool for measuring burnout among workers.
Risk of Falls Associated with Long-Acting Benzodiazepines or Tricyclic Antidepressants Use in Community-Dwelling Older Adults: A Nationwide Population-Based Case–Crossover Study
Authors: Na Inyoung , Seo Junyoung , Park Eunjin , Lee Jia
Published: July 2022

Background: Falls are common in older adults and increase in recent years. This study aimed to examine the risk of falls associated with long-acting benzodiazepines (BZDs) or tricyclic antidepressants (TCAs) use in community-dwelling older adults. Methods: A nationwide population-based case–crossover design was used. We screened information on 6,370,275 fall or fall fracture cases among community-dwelling elderly patients from the database of the national health insurance data warehouse in South Korea. We extracted the data of elderly patients who visited the hospital for a fall and were diagnosed with the first fall or fall fracture after prescription of long-acting BZDs (n = 1805) or TCAs (n = 554). The study used conditional logistic regression analysis to analyze the associations and stratified analysis by gender and age group to control for their confounding effects. Results: Risk of falls or fall fractures increased by more than two times after taking long-acting BZDs (odds ratio [OR] = 2.16; 95% confidence interval [CI] = 1.85–2.52) or TCAs (OR = 2.13; 95% CI = 1.62–2.83). The longer the prescription period of both, the higher the risk of falls or fall fractures was (≥49 days for long-acting BZDs vs. ≥ 56 days for TCAs). Conclusions: Long-acting BZDs or TCAs should be avoided or prescribed for a shorter duration based on these adverse effects. Health care providers should focus on fall prevention practices in older adults who take such drugs.
Mapping the Sustainable Human-Resource Challenges in Southeast Asia’s FinTech Sector
Authors: Wu An-Chi , Kao Duc-Dinh
Published: July 2022

The significance of human resources (HRs) has increased with the increasing awareness of sustainability issues and corporate social responsibility. However, the rapidly emerging financial technology (FinTech) sector still presents an HR challenge. Southeast Asia, which accounts for the highest adoption rate of mobile banking, has set new records regarding the number of transactions, as well as funding amount, in recent years. Moreover, borderless financial cooperation, coupled with in-demand tech talents, will rapidly boost the development of the region. Thus, this study explored the new opportunities as well as challenges of a new business model, FinTech, in Southeast Asia’s banking and enterprise sector in the post-COVID-19 era. It also examined how organizations can achieve sustainable development via the interaction of the new operating model with existing ones by developing relevant strategies in the context of the “new normal” working condition. By reviewing the literature on HR management (HRM), we proposed how banking and FinTech companies could supply tech talent with the relevant experience or engage in training projects before recruiting. Additionally, since organizations desire sustainability-minded employees, they offer flexible working arrangements and well-established reward policies that can create remote work performance and retention rates. Being committed to upskilling and reskilling global talent by offering talent mobility opportunities across the organization, as well as by fully embracing the creation of value for cross-cultural talent, companies can support their employees’ long-term career goals and maintain competitive strength. Finally, organizations must focus more on flexible adjustments and cross-domain communication for global talent. Forming strategic alliances with FinTech companies would be an alternative conduit that can ensure that regional laws comply with the local culture and national law, for bias and conflict reduction.
Resistance to Cybersecurity Attacks in a Novel Network for Autonomous Vehicles
Authors: Brocklehurst Callum , Radenkovic Milena
Published: July 2022

The increased interest in autonomous vehicles has led to the development of novel networking protocols in VANETs In such a widespread safety-critical application, security is paramount to the implementation of the networks. We view new autonomous vehicle edge networks as opportunistic networks that bridge the gap between fully distributed vehicular networks based on short-range vehicle-to-vehicle communication and cellular-based infrastructure for centralized solutions. Experiments are conducted using opportunistic networking protocols to provide data to autonomous trams and buses in a smart city. Attacking vehicles enter the city aiming to disrupt the network to cause harm to the general public. In the experiments the number of vehicles and the attack length is altered to investigate the impact on the network and vehicles. Considering different measures of success as well as computation expense, measurements are taken from all nodes in the network across different lengths of attack. The data gathered from each node allow exploration into how different attacks impact metrics including the delivery probability of a message, the time taken to deliver and the computation expense to each node. The novel multidimensional analysis including geospatial elements provides evidence that the state-of-the-art MaxProp algorithm outperforms the benchmark as well as other, more complex routing protocols in most of the categories. Upon the introduction of attacking nodes however, PRoPHET provides the most reliable delivery probability when under attack. Two different attack methods (black and grey holes) are used to disrupt the flow of messages throughout the network and the more basic protocols show that they are less consistent. In some metrics, the PRoPHET algorithm performs better when under attack due to the benefit of reduced network traffic.
Research on Mechanical Behavior of the Steel–Concrete–Steel Composite Structures Subjected to High Temperature of Fire
Authors: Cao Peng , Hu Xuebing , Liu Enlong , Chen Jianzhong , Jiang Shouchao , Ding Hao
Published: July 2022

A new type of steel–concrete–steel composite structure was adopted and widely used in the immersed tunnel of the Shenzhen–Zhongshan access. The research on the mechanical behavior of the new composite structure under a high temperature of fire is of great engineering significance to the fire protection design of the structure. Both the model test and a numerical simulation were adopted to study the mechanical behavior and damage characteristics of the new composite structure under fire. The RABT standard temperature rise curve was used to simulate the temperature rising law under fire (it reflects the characteristics of temperature rise in case of fire in an enclosed environment: rapidly raised to 1200 °C within 5 min, maintained at 1200 °C for 120 min, then it is cooled to normal temperature after 110 min). The temperature distribution law inside the structure, the deformation development law of the roof and the crack distribution were analyzed based on the thermal–mechanical coupling analysis method. The results showed that the internal part of the composite structure close to the fire surface was directly affected by the high temperature, and the temperature presented a step distribution law, while the part far from the fire surface was affected by the lag effect of the temperature transfer, and the temperature presented a continuous growth law. The roof deformation presented a three-stage model of “rapid growth-deformation stability-deformation recovery” with time. The overall cracks of the composite structure showed a symmetrical distribution under fire. The composite structure presented a shear failure mode as a whole. The results could provide a reference for the study of fire resistance for the new composite structure and support the structural fire protection design of the immersed tunnel of the Shenzhen–Zhongshan access.
Investigation of Cytotoxicity of Biosynthesized Colloidal Nanosilver against Local Leishmania tropica: In Vitro Study
Authors: Zein Raghad , Alghoraibi Ibrahim , Soukkarieh Chadi , Alahmad Abdalrahim
Published: July 2022

Leishmaniasis is one of the biggest health problems in the world. Traditional therapeutic methods still depend on a small range of products, mostly chemically. However, the treatment with these drugs is expensive and can cause serious adverse effects, and they have inconsistent effectiveness due to the resistance of parasites to these drugs. The treatment of leishmanial disease has always been a challenge for researchers. The development of nanoscale metals such as silver has attracted significant attention in the field of medicine. The unique characteristic features of silver nanoparticles (AgNPs) make them effective antileishmanial agents. In recent years, green nanotechnology has provided the development of green nanoparticle-based treatment methods for Leishmaniasis. Although there are many studies based on green nanoparticles against Leishmania parasites, this is the first study on the antileishmanial effect of biosynthesized AgNPs using an aqueous extract of Eucalyptus camaldulensis leaves (AEECL) as a reducing agent of silver ions. Different parameters such as AgNO3 concentration, AEECL concentration, and reaction time were studied to investigate the optimum factors for the preparation of stable and small-sized silver nanoparticles. The spherical shape of colloidal nanosilver (CN-Ag) was confirmed by atomic force microscope (AFM) and scanning electron microscope (SEM) images with sizes of 27 and 12 nm, respectively. A high density of nanoparticles with a small size of 10 nm has been confirmed from dynamic light scattering (DLS) analysis. The zeta potential value of 23 mV indicated that colloidal silver nanoparticles were stable. The nano-tracker analysis (NTA) showed the Brownian motion of silver nanoparticles with a hydrodynamic diameter of 31 nm. The antioxidant property of CN-Ag was determined using the stable radical 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. In this study, a significant cytotoxic effect of biosynthesized CN-Ag has been shown against Leishmania tropica parasites at low concentrations (1.25, 2.5, and 3.75 µg/mL). These results could be used as a future alternative drug or could be a supportive treatment for Leishmaniasis.
The Use of Electrode Tools Obtained by Selective Laser Melting to Create Textured Surfaces
Authors: Ablyaz Timur Rizovich , Shlykov Evgeny Sergeevich , Muratov Karim Ravilevich
Published: July 2022

The study and development of the technological foundations for creating a textured surface using an electrode tool obtained by the method of additive manufacturing are the purpose of the work. Methods for obtaining textured surfaces and for creating a tool electrode for electrical discharge machining are considered in this work. The modeling of the electrodetool, analysis of internal stresses during its manufacture by the selective laser melting method, and the manufacture of electrodes are considered. A Realizer SLM 50 laser machine was used to create the electrode tool. Ti6Al4V metal powder with an average particle size of 30 µm was chosen as the material for manufacturing. The experiments were carried out on a copy-piercing electrical discharge Smart CNC machine. The material of the workpiece is corrosion-resistant, heat-resistant, high-alloy steel 15Cr12H2MoWVNNB. An Olympus GX 51 light microscope (Olympus Corporation, Shinjuku-ku, Japan) at 100× magnification was used to visually evaluate the texturing results and measure dimensions. The possibility of using electrodes obtained by the selective laser melting method for texturing surfaces was studied.
Study on Correlation of Mechanical and Thermal Properties of Coal-Based Carbon Foam with the Weight Loss Rate after Oxidation
Authors: Wang Degang , Zhuang Qikai , Li Kai , Wang Yanfei
Published: July 2022

With the increasing demand for high temperature-resistant heat insulation materials for hypersonic vehicles, carbon foam has been studied extensively, and its mechanical and thermal properties have been fully researched, but the oxidation behavior of carbon foams during service and the change in their properties after oxidation are rarely studied. This paper studied the relationship between both mechanical and thermal properties and oxidation degree of two kinds of foams, coal-based carbon foam and antioxidant coal-based carbon foam with chemical vapor deposition of SiC particles. The variation trend for the two kinds of foam was the same. When the oxidation degree was less than 15%, the compression modulus and strength weakened with the increase in weight loss rate, but the thermal conductivity decreased with the increase in weight loss rate, which was a favorable influence for the thermal protection system. The mechanical and thermal properties had a linear relationship with the weight loss rate, but the slope was different between 0% to 10% and 10% to 15%. The microscopic mechanism of these changes was also analyzed.
Successful Endovascular Surgery for Iatrogenic Common Iliac Artery Injury during Lumbar Spine Surgery: A Case Report
Authors: Chin Chien-Ming , Liu Kuan-Lin , Chen Ing-Ho
Published: July 2022

An 80-year-old man was admitted with an L5 compression fracture, L4/5 spondylolisthesis, and L5 radiculopathy and underwent a TLIF procedure. Refractory hypotension occurred, though it indicated a possible great vessel injury with vasopressor and fluid infusion. Emergent intraoperative angiography was performed, which showed extravasation at the right common iliac artery. Resuscitative endovascular balloon occlusion of the aorta followed by right common iliac artery stenting was successfully performed to arrest the bleeding. The iatrogenic right common iliac artery laceration was complicated with abdomen compartment syndrome and acute kidney injury. The patient received supportive care, including continuous venovenous hemofiltration (CVVH) for a week, after which the patient’s condition improved. The patient did not have any residual complications at the one-month follow-up. Great vessel injury during the TLIF procedure is rare but fatal. Refractory hypotension is indicative of a great vessel injury. Endovascular intervention is a fast and promising method to diagnose and treat arterial injury.
Failure Analysis of a Chromium Plating Layer on a Piston Rod Surface and the Study of Ni-Based Composite Coating with Nb Addition by Laser Cladding
Authors: Wang Jiangang , Zhang Qiankun , Shen Wenjing , Liang Zhimin , Chang Chaoqiang , Yang Liyong , Li Jianhui , Huang Fengshan
Published: July 2022

The failure of a chromium plating layer on the surface of a piston rod was analyzed, and an Ni-based alloy mixed with a niobium (Nb) composite coating was investigated by means of stereomicroscopy, metallographic microscopy, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Vickers hardness testing, and wear testing. The results show that penetrating cracks were present in the chromium layer. Subsequently, the corrosion intensified, resulting in the bubbling, cracking, and peeling of the chromium layer. The cladding layer presented a structure morphology of planar crystalline at the bottom, dendritic at the middle, and equiaxial crystalline at the top. The solidification parameters, which were derived from simulation results, confirmed that the ratios between temperature gradient (G) and solidification speed (S) decreased, and the values of the cooling speeds increased from the bottom to the top of the cladding layer. With an increase in Nb content, the structure became gradually refined and uniformly dense. The cladding layer of the Ni-based alloy mixed with Nb was mainly composed of γ-Ni, Cr23C6, Cr7C3, NbC, and Ni3Nb. NbC could be formed in situ and presented itself in four forms: particles, dendrites, polyhedrals, and networks. The microhardness of the coating with 15% added Nb was enhanced to 400 HV0.2, and the wear resistance thereof was 11.14 times higher than the substrate. After the 15% Nb coating had aged for 16 h, the diffraction peak intensities of Cr23C6 and Cr7C3 significantly increased, and the volume fraction of granular NbC increased from 1.67% to 2.54%. The microhardness was enhanced to 580 HV0.2, and the wear resistance thereof was better than that of the chromium plating layer.
Development and Validation of Nutrition Literacy Assessment Instrument for Chinese Pregnant Women
Authors: Zhou Yalin , Lyu Ying , Zhao Runlong , Shi Hanxu , Ye Wanyun , Wen Zhang , Li Rui , Xu Yajun
Published: July 2022

The study was designed to develop and validate the nutrition literacy assessment instrument for pregnant women in China (NLAI-P). The dimension, components and questions of NLAI-P were identified via literature review and expert consultation. A panel of experts evaluated the content validity. The construct validity was evaluated by using the exploratory factor analyses (EFA) and confirmatory factor analyses (CFA). Cronbach’s α coefficient and split-half reliability were applied for examining the reliability. The NLAI-P was divided into 3 dimensions including knowledge, behavior and skill dimension. Findings showed NLAI-P possessed the satisfactory content validity (content validity index = 0.98, content validity ratio = 0.97), acceptable construct validity (χ2/df = 1.82, GFI = 0.86, AGFI = 0.84, RMSEA = 0.046) and good reliability (Cronbach’s α coefficient = 0.82). The average scores of NLAI-P were 46.59 ± 9.27. With the adjustment of confounding factors, education level presented a significantly positive correlation with NLAI-P scores. In conclusion, NLAI-P were valid and reliable to inspect NL level of pregnant women in China. Poor NL was prevalent among Chinese pregnant women. Based on the education level, taking targeted propaganda and education measures would achieve the optimal effect. NLAI-P can be applied as the tool for monitoring and assessing NL of pregnant women, and facilitate the designation of targeted interventions policies.
Tomato By-Products, a Source of Nutrients for the Prevention and Reduction of Malnutrition
Authors: Kaboré Kabakdé , Konaté Kiéssoun , Sanou Abdoudramane , Dakuyo Roger , Sama Hemayoro , Santara Balamoussa , Compaoré Ella Wendinpuikondo Rakèta , Dicko Mamoudou Hama
Published: July 2022

Malnutrition is a global phenomenon that mainly affects children under five years old, the elderly and food insecure people. It can be linked to undernourishment or overnutrition. To prevent it, a healthy and balanced diet, rich in energy and micronutrients, is necessary. The objective of this study was to evaluate the nutritional composition and contribution of tomato by-products to prevent and reduce malnutrition. Thus, standard methods were used for proximate composition and bioactive compounds. Results showed that tomato by-products are rich in macronutrients and micronutrients capable of preventing undernutrition and reducing the incidence of the effects of overnutrition. The average carbohydrate, protein and lipid contents were 15.43%, 11.71% and 5.4% (DM) in peels and for seeds the contents were 58.75%, 15.4% and 22.2%, respectively. The average energy values were 280.47 kcal/100 g and 472.8 kcal/100 g DM for peels and seeds, respectively. The main minerals found were, in decreasing order, potassium, magnesium, sodium, iron and zinc. High contents of phenolic compounds, lycopene, β-carotene and vitamin C were also found in these by-products. They also presented important antioxidant activities. Due to their nutritional and bioactive compounds, tomato by-products may be included in functional food formulation programs to reduce the incidence of nutritional diseases.
Effect of Flaxseed (Linum usitatissimum L.) Supplementation on Vascular Endothelial Cell Morphology and Function in Patients with Dyslipidaemia—A Preliminary Observation
Authors: Kanikowska Dominika , Malińska Agnieszka , Mickiewicz Agnieszka , Zawada Agnieszka , Rutkowski Rafał , Pawlaczyk Krzysztof , Sato Maki , Bręborowicz Andrzej , Witowski Janusz , Korybalska Katarzyna
Published: July 2022

Context: Flaxseed has a characteristic fatty acids composition and unique phytonutrient profile that may have health-promoting properties. Objective: This study aimed to determine the effects of 10 weeks of supplementation with the flaxseed (28 g/day) on endothelial cells (EC) function, serum lipids and proinflammatory mediators in patients with mild and severe dyslipidaemia. Materials and methods: Eleven lean patients with severe dyslipidaemia treated with apheresis (group 1; 10 weeks treated in four phases: (i) ordinary diet, (ii) ordinary diet + flaxseed, (iii) ordinary diet (wash out), (iv) ordinary diet + placebo) and eleven obese patients with mild dyslipidaemia—not treated with apheresis (group 2; 10 weeks treated in two phases: (i) ordinary diet, (ii) low fat diet + flaxseed). Flaxseed was given blindly. Serum was collected at the end of each phase of the study. ECs were exposed in vitro to the medium supplemented with pooled serum taken from patients from both groups to detect their morphological changes using light and electron microscopy. ECs proliferation was also measured at the end of each study phase. Results: Serum vascular endothelial growth factor was decreased after flaxseed supplementation but only in group 1. ECs proliferation was increased after flaxseed supplementation only in obese patients. ECs exposed to medium supplemented with obese patients’ serum revealed the following cellular abnormalities: accumulation of lipid droplets, changes of rough endoplasmic reticulum and mitochondria, and flaxseed did not reverse observed changes. At the same time, flaxseed supplementation decreases total cholesterol in both tested groups, low-density lipoprotein cholesterol in group 1 and triglycerides in group 2. Conclusions: Our findings support the potential role of flaxseed in treating dyslipidaemia but indicate only a slight impact on endothelial cell function.
Computer Simulation of Polyethylene Terephthalate Carbonated Beverage Bottle Bottom Structure Based on Manual–Automatic Double-Adjustment Optimization
Authors: Ge-Zhang Shangjie , Chen Xiaoli , Zhu Haotong , Song Yuan , Ding Yuyang , Cui Jingang
Published: July 2022

PET bottlesare often used as airtight containers for filling carbonated drinks. Because carbonated drinks contain large volumes of CO2 gas, the container needs to bear a tremendous pressure from the inside of the bottle.If the stress exceeds the bearing limit, the material will show the phenomenon of local cracking and liquid overflow.For the structural design, the method of manual adjustment before automatic adjustment was adopted. First, through manual optimization, the initial optimal parameter combination was as follows:the inner diameter of the bottle bottom was 17 mm, the dip angle of the valley bottom was 81°, the deepest part of the valley bottom was 25 mm, and the outer diameter was 27 mm. Comsol software was used for automatic optimization. Compared with the original bottle bottom, the total maximum principal stress and total elastic strain energy in the bottle bottom after manual–automatic double optimization decreased by 69.4% and 40.0%, respectively, and the displacement caused by deformation decreased by 0.60 mm (74.1%). The extremely high reduction ratio was caused by manual–automatic double optimization.