bluuebunny/crossref_metadata_2025 huggingface dataset:
Dexmedetomidine Inhibits Nlrp3 Inflammasome Priming in BV-2 microglia through the NF-κB Pathway and the ROS-Nlrp3-IL-1β Signaling Axis
Authors: Xie Jiawei , Chen Li , Luo Yuling , Li Jianling , Wang Xianxue , Tang Jieke , Tian He , Liang Shuqing , Xi Biao , Li Yalan
Published: September 2021

In the brain, the NOD-like receptor family pyrin domain containing 3 (Nlrp3) inflammasome is mostly expressed in microglia and is considered to be the primary cause of perioperative neurocognitive dysfunction (PND). Dexmedetomidine (Dex), a novel kind of clinical anesthetic with anti-inflammatory properties, has been shown to be effective in preventing PND in surgical patients. However, the mechanism of its anti-neuroinflammatory activity is still quite unclear. We examined the impact of Dex administration on Nlrp3 priming in activated BV-2 cells in this research. To investigate the mechanism by which Dex impacts Nlrp3 priming, we employed the inhibitors pyrrolidine dithiocarbamate (PDTC) and N-acetyl-L-cysteine (NAC) to block the NF-κB p65 and the reactive oxygen species (ROS)-Nlrp3-interleukin (IL)-1β signaling axis, respectively. The results showed that Dex substantially decreased the expression of Nlrp3 and p65 and significantly inhibited the levels of the inflammatory factors IL-1β and tumor necrosis factor (TNF)-α in BV-2 cells stimulated with lipopolysaccharide (LPS). Additionally, when the NF-κB pathway was inhibited by PDTC, Dex could aggravate the downregulation of Nlrp3 and IL-1β in BV-2 cells. What is more, Dex negatively regulated the expression of Nlrp3 and IL-1β in activated BV-2 cells when NAC was added. These results showed that Dex inhibited Nlrp3 priming in LPS-induced BV-2 cells, presumably via blocking the NF-κB pathway and the ROS-Nlrp3-IL-1β signaling axis.
Psychometric Properties of The Persian Version of Short-Form Five Factor Borderline Inventory (FFBI-SF)
Authors: Athar Mojtaba Elhami , Jazi Elham Azamian , Lashgari Zahra , Azizi Morteza , Karimi Sirvan , DeShong Hilary L.
Published: September 2021

Background
The Five-Factor Borderline Inventory-Short Form (FFBI-SF) is a self-report measure developed to assess traits of Borderline Personality Disorder (BPD) from the perspective of the Five-Factor Model of general personality. This study was designed to examine the factor structure, internal consistency, and convergent/discriminant validity of the Persian FFBI-SF in a sample of Iranian university students.
Methods
A total of 641 university students (M-age = 28.04, SD = 8.21, 66.7% women) completed the online forms of the FFBI-SF, PID-5-BF, and Mini IPIP.
Results
Confirmatory factor analysis supported the proposed twelve-factor model. Also, Cronbach's alpha (α) for the FFBI-SF scores ranged from unacceptable to excellent ranges. However, when relying on MIC values to measure internal consistency, the FFBI-SF Total and subscale scores demonstrated adequate internal consistency. Finally, the FFBI Total and subscale scores showed the expected relations with external correlates (e.g., Neuroticism, Antagonism, and Conscientiousness), which supports the validity of the interpretation of the FFBI-SF scores.
Conclusions
The findings indicated that FFBI-SF is a useful tool with sound psychometric properties for assessing BPD traits in Iranian students and may spark research in other Iranian settings (e.g., clinical samples).
Advanced Paternal Age With Risk of Lifespan in Male Offspring Using Chinese Genealogical Data
Authors: xiong yanyan , Zhu Xueqin , Xie Ying
Published: September 2021

Background Advanced paternal age has been associated with a variety of adverse reproductive outcomes. However, the effects of paternal age on offspring lifespan are still controversial. Result Here, we studied the correlation between parental reproductive age and offspring lifespan using a Chinese specific genealogy data. We chose a “Ding” genealogy data across 130 years during the Qing Dynasty (1726-1855). The present study showed that fathers aged more than 35 years were more likely to have male offspring with lower lifetime compared with fathers aged 20-35 years. The significant negative correlation between paternal age and male offspring lifespan existed after adjusted for maternal age. We proposed a new evidence that advanced paternal age is a risk for male offspring survival.
Risk Prediction of Hepatocellular Carcinoma in Patients With Alcoholic Cirrhosis in an Area With Intermediate Prevalence for Hepatitis B Virus Infection: a Competing Risk Analysis
Authors: Lee Kyunghan , Choi Gwang Hyeon , Jang Eun Sun , Jeong Sook-Hyang , Kim Jin-Wook
Published: September 2021

Background & Aims: The role of hepatocellular carcinoma (HCC) surveillance is being questioned in alcoholic cirrhosis because of the relative low incidence of HCC. Comorbid viral hepatitis may modify the risk, and competing outcomes may influence the actual incidence of HCC in alcoholic cirrhosis. This study aimed to assess the risk and predictors of HCC in patients with alcoholic cirrhosis by using competing risk analysis in an area with intermediate prevalence for hepatitis B virus.Methods: A total of 965 patients with alcoholic cirrhosis were recruited at a university-affiliated hospital in Korea and randomly assigned to either the derivation (n=643) and validation (n=322) cohort. Subdistribution hazards model of Fine and Gray was used with deaths and liver transplantation treated as competing risks. Death records were confirmed from Korean government databases. A nomogram was developed to calculate the Alcohol-associated Liver Cancer Estimation (ALICE) score.Results: Markers for viral hepatitis were positive in 21.0 % and 25.8 % of patients in derivation and validation cohort, respectively. The cumulative incidence of HCC was 13.5 and 14.9 % at 10 years for derivation and validation cohort, respectively. Age, positivity for viral hepatitis markers, alpha-fetoprotein level, and platelet count were identified as independent predictors of HCC and incorporated in the ALICE score, which discriminated low, intermediate, and high risk for HCC in alcoholic cirrhosis at the cut-off of 120 and 180. Conclusions: ALICE score reliably stratifies HCC risk of alcoholic cirrhosis in an area where the prevalence of viral hepatitis is substantial.
The Impact of Online Design Thinking-Based Learning On Entrepreneurial Intention: The Case of Vocational College
Authors: Woraphiphat Issariya , Roopsuwankun Pattama
Published: September 2021

Entrepreneurial intention has been found to be fundamental to decision-making and behavior to become entrepreneurs, with consequent effects on economic development. However, the COVID-19 pandemic calls for a novel approach to teaching entrepreneurship owing to the shift to online learning. The current study explores entrepreneurial intention derived from the entrepreneurship education program (EEP) using an online group-based learning approach. In particular, we examine satisfaction with an online group-based learning approach by integrating the theory of planned behavior with design thinking-based entrepreneurship course, peer interactions, and speaker interactions. The entrepreneurship education program (EEP) in this research was for vocational college students located in Southeast Asia. The online questionnaire was distributed to participants (N = 263, MAGE = 18.64) at the end of the online entrepreneurship education program. The model was tested using a structural equation model analysis. Attitude, subject norm, and satisfaction were found to predict higher entrepreneurial intention among vocational college students. Moreover, design thinking-based entrepreneurship course, peer interaction, and speaker interaction have indirect effects on entrepreneurial intention through satisfaction. This research extends the literature on entrepreneurship education by proposing a novel learning approach, that is, the online groupbased learning approach, which could be applied to entrepreneurship education programs to enhance students’ entrepreneurial intention.
Transfer of Cleavage-Stage Embryos May Benefit Normal Responders With Low Rate of Morphologically Good Embryos Formation On Day 3: A Comparison Study
Authors: Dai Xiuliang , Xia Xiyang , Gao Tingting , Yu Chunmei , Cao Fang , Li Tianfu , Li Lingjun , Wang Yufeng , Chen Li
Published: September 2021

Background
Do morphologically good (MG) embryos from patients with high and low rate of MG embryos on day 3 (RMD3) show similar developmental potential (DP)?
Methods

This respective study finally included a total of 916 fresh cycles and related 1074 FET cycles from Jan 2017 to May 2020 in our reproductive center. Cycles with high RMD3 were defined as the H group, while cycles with low RMD3 were defined as the L group. The basic characteristics of patients and fresh cycles, blastulation rate, and clinical outcomes were compared between the H and L groups in either ET cycles with MG day 3 cleavage embryos (ETC group) or ET cycles with MG blastocysts (ETB group).
Results

The overall characteristics of patients and cycles were grossly comparable between the H and L groups either in ETC or ETB groups. In ETB group, useable blastocysts formation rate, implantation rate and live birth rate was significantly reduced in the L group, compared to the H group;In ETC group, useable blastocysts formation rate was significantly reduced in the L group. However, implantation rate and livebirth rate was similar between the L and H groups.
Conclusion

The in vitro DP of MG day 3 embryos and in vivo DP of MG blastocysts were reduced significantly, while a similar in vivo DP of MG day 3 embryos was observed in patients with low RMD3 as compared to patients with high RMD3. It seems that direct transfer of day 3 MG embryos instead of extended culture may benefit patients with RMD3.
Factors Affecting People’s Environmental Awareness In The Urban Areas: A Case of Addis Ababa, Ethiopia
Authors: Getachew Dagne , Assefa Engdawork , Seyoum Abrham
Published: September 2021

Background: In developing countries, the urban environment is deteriorating over time. In the meantime, people's demand for clean and green residential and recreational places has increased. If so, why has it been hard to keep clean and green cities? This paper investigates the level and determinants of environmental awareness in Addis Ababa. From three sub-cities, a three-stage sampling procedure applies to select 293 sample respondents. The data collection applies a structured questionnaire. We applied a five-point Likert scale to classify the levels of awareness. Besides, an ordered logit model was applied to analyze factors that affect the level of awareness.Results: The result shows that the knowledge of forest degradation is high, while the attitude to reduce the use of glass bottles is medium. The cognitive skill on the cause of acid rain is medium. The probability of low (13%) and medium (26%) levels of environmental awareness increases for the income group of 601 to 1650. Likewise, the likelihood of having low levels of environmental awareness rises by 9%; in contrast, the probability of having a moderate level of environmental awareness increases by 12% for the age of 50 to 59. The TVET educational level has a low chance of having low (8%) and medium (12%) levels of awareness.Conclusions: An income-generating activity raises employment opportunities and creates a better income, which would influence the environmental mindset. So, improving the living standard assures clean and green cities. Besides, the higher the education, the better would be environmental knowledge, cognitive skills, and attitude. In the meantime, besides formal education, adult education, training, and workshops are alternatives to enhance environmental awareness.
The Impact of Design Elements on Undergraduate Nursing Students’ Educational Outcomes in Simulation Education: Protocol for a Systematic Review
Authors: Jackson Matthew , McTier Lauren , Brooks Laura A , Wynne Rochelle
Published: September 2021

Background: Although simulation-based education (SBE) has become increasingly popular as a mode of teaching in undergraduate nursing courses its effect on associated student learning outcomes remains ambiguous. Educational outcomes are influenced by SBE quality that is governed by technology, training, resources and SBE design elements. This paper reports the protocol for a systematic review to identify, appraise and synthesise the best available evidence regarding the impact of SBE on undergraduate nurses’ learning outcomes. Methods: Databases to be searched from 1st of January 1990 include the Cumulative Index to Nursing and Allied Health Literature (CINAHL), the Medical Literature Analysis and Retrieval System Online (MEDLINE), American Psychological Association (APA) PsycInfo and the Education Resources Information Centre (ERIC) via the EBSCO host platform. The Excerpta Medica database (EMBASE) will be searched via the OVID platform. We will review the reference lists of relevant articles for additional citations. A combination of search terms including ‘nursing students’, ‘simulation training, ‘patient simulation’, and ‘immersive simulation’ with common Boolean operators will be used. Specific search terms will be combined with either MeSH or Emtree terms and appropriate permutations for each database. Search findings will be imported into reference management software (Endnote© Version.X9) then uploaded into Covidence where two reviewers will independently screen titles, abstracts and retrieved full text. A third reviewer will be available to resolve conflicts and moderate consensus discussions. Quantitative primary research studies evaluating the effect of SBE on undergraduate nursing students’ educational outcomes will be included. The Mixed Methods Appraisal Tool (MMAT) will be used for quality assessment of core criteria, in addition to the Cochrane RoB 2 and ROBINS-I to assess risk of bias for randomised and non-randomised studies, respectively. Primary outcomes are any measure of knowledge, skills, or attitude. Discussion: SBE has been widely adopted by healthcare disciplines in tertiary teaching settings. This systematic review will reveal (i) the effect of SBE on learning outcomes, (ii) SBE element variability, and (iii) interplay between SBE elements and learning outcome. Findings will specify SBE design elements to inform the design and implementation of future strategies for simulation-based undergraduate nursing education.Systematic Review Registration: PROSPERO CRD42021244530
Mapping of Natural Radioactivity in Soil Samples of Kufa Districts, Iraq Using GIS Technique
Authors: Alasadi Lubna A. , Abojassim Ali Abid
Published: September 2021

The aim from this work is to detect and draw the natural radioactivity of (uranium-238, thorium-232 and potassium-40) levels in soil samples that taken from Kufa districts, Najaf governorate, Iraq. The detection system used in study is NaI (Tl) with "3x3" dimension, while maps were drawn by geographic information system (GIS). Some radiological parameters were calculated depending on measuring of specific activity for 238U, 232Th, and 40K. The results of the average value with standard error in all samples of the present study for 238U, 232Th, 40K, and 235U were 6.2±0.74, 6.41±0.82, 278.10±19.43, and 0.28±0.03 Bq/kg, respectively. The specific activity of Uranium-238, Thourium-232, poasium-40 and Uranium-235 found in all soil samples were within the safety limit reported by UNSCEAR, except three samples have poasium-40 higher than safety limit. Also, the results of radiological parameters were all found to be below OECD, UNSCEAR safety limit. Generally, the average of specific activity in soil collected arranged of potassium-40 > thorium-232 > uranium-238. Mapping GIS for natural radioactivity and some radiological parameters were successfully draw for the study area. Finally, natural radioactivity and radiological parameters for soil samples at Kufa districts were safety.
Self-healing Co3O4@Fe3O4/cellulose Blend Membranes Efficient Degrade Perfluorooctanoic Acid in the Visible Light-driven Photo-fenton System
Authors: Chen Wenqiang , Hou Chen , Wang Yang , Fu Linhui , Zhang Sufeng , Wang jianzhi
Published: September 2021

Co3O4@Fe3O4/cellulose was synthesized by in-situ self-assembly strategy coating rod-like MOF-derived Fe3O4 with Co3O4 nanoparticles and blending with cellulose solution, further applied in the visible light-driven photo-Fenton system for PFOA degradation. In addition, Co3O4@Fe3O4/cellulose/Vitrimer was obtained to explore the application of self-healing property in photo-Fenton filed and the result turned out to be good self-healing capacity for small cracks. In comparison, Co3O4@Fe3O4/cellulose can degrade around 94.5% PFOA within 180 min in reaction system, which shows better degradation capacity than others catalyst. Moreover, Co3O4@Fe3O4/cellulose was reused by rinsing with ultra-pure water and the degradation capacity was still 80.4% after five cycles. In this system, the results of Electron paramagnetic resonance analysis (EPR) and scavenger experiment suggested that PFOA degradation was a co-dependent mechanism via photogenerated electrons, photogenerated holes (h+) and various radical species, rather than a single active constituent. The degradation pathway of PFOA also was proposed based on UHPLC-MS analysis.
Dynamics and Functional Interplay of Non-histone Lysine Crotonylome and Ubiquitylome in Vascular Smooth Muscle Cell Phenotypic Remodeling
Authors: Cao Shan-Hu , Chen Zhi-Huan , Yue Lin , Jiang Han-Mei , Ma Ru-Yuan , Dong Li-Hua
Published: September 2021

BackgroundsCrotonylation of histones is a recently discovered type of post-translational modification that can regulate gene expression. However, the function of crotonylation on nonhistone proteins in vascular smooth muscle cells (VSMC) is unclear. Here, we aim to use modification and proteomic analysis to find the cellular characteristic of crotonylated nonhistone proteins and the crosstalk with ubiquitinated proteins in vascular smooth muscle cell (VSMC) phenotypic remodeling. ResultsWe performed modification and proteomic analysis of VSMCs before and after platelet-derived growth factor-BB (PDGF-BB) stimulation. The crotonylated and ubiquitinated pan-antibody was used to enrich the protein and then subjected to high-throughput mass spectrometry analysis. Then we compared the enrichment analysis of differentially modified proteins in regards to GO terms, KEGG pathway and protein domain. As a result, there were 2138 crotonylation sites in 534 proteins and 1359 ubiquitination sites corresponding to 657 proteins. The crotonylated proteins participate in a variety of important cellular pathways and perform different functions in VSMCs. Among them, some proteins were found to be closely involved in the physiological process of VSMC phenotypic remodeling including glycolysis/gluconeogenesis, vascular smooth muscle contraction, and PI3K-Akt signaling pathway. Furthermore, the KEGG pathway enrichment analysis showed that the ubiquitinated proteins were found to be closely involved in the physiological process of VSMC phenotypic remodeling including glycolysis/gluconeogenesis, vascular smooth muscle contraction, RAS signaling pathway or PI3K-Akt signaling pathway. Crosstalk analysis showed that there were 199 sites within 177 proteins modified by crotonylation and ubiquitination simultaneously. PPI network analysis indicated that crotonylated and ubiquitinated proteins played an important role in cellular bioprocess commonly and possible synergistic effect. ConclusionsIn summary, our bioinformatics show that nonhistone crotonylation and ubiquitination play an important role in the VSMC phenotypic transformation induced by PDGF-BB stimulation. The crosstalk of crotonylation and ubiquitination in glycolysis is possibly a novel mechanism underlying the VSMC phenotypic remodeling.
Validation of Acromegaly Quality of Life Questionnaire (AcroQol) for the Iranian Population
Authors: Danaei Mina , Bahadorizadeh Leyla , Dehnad Afsaneh , Mohamadzadeh Shirin , Hashemi-Madani Nahid , Khamseh Mohammad E
Published: September 2021

Background: Acromegaly is a chronic disease seriously impacting the patients' quality of life. This study aimed to validate the Acromegaly Quality of Life Questionnaire (AcroQoL) for the Persian-speaking population.Methods: This cross-sectional study recruited 73 Iranian patients with a confirmed diagnosis of acromegaly. The content validity of the scales was evaluated by an expert panel of eight endocrinologists. Construct validity was assessed by using confirmatory factor analysis. Internal consistency was assessed on the basis of Cronbach’s alpha, and a goodness-of-fit (GoF) index was calculated to display whether the model fitted the data.Results: The content validity index (CVI) and content validity ratio (CVR) yielded values of 0.85 and 0.80, respectively. The average variance extracted (AVE) from physical and psychological dimensions was 0.520 and 0.462, respectively, exceeding the minimum criterion of 0.40. Cronbach’s alpha for physical and psychological dimensions equaled 0.868 and 0.866, respectively, indicating the adequate internal consistency of multiple items for each construct. The subscales’ R square and path coefficient were greater than the recommended threshold (physical dimension: 0.778, psychological dimension: 0.873), demonstrating the suitability of this criterion. Finally, the GoF value of 0.29 indicated the model's moderate fit.Conclusions: The findings revealed that the Persian version of AcroQoL is of adequate validity and reliability for evaluating the quality of life of Iranian people with acromegaly.
A Capra Hircus Chromosome 19 Locus Linked to Milk Production Influences Mammary Conformation
Authors: Jiang Andrew , Ankersmit-Udy Alex , Turner Sally-Anne , Scholtens Megan , Littlejohn Mathew D , Lopez-Villalobos Nicolas , Proser Colin G , Snell Russell G , Lehnert Klaus
Published: September 2021

Background: Economically important milk production traits including milk volume, milk fat and protein yield vary considerably across dairy goats in New Zealand. A significant portion of the variation is attributable to genetic variation. Discovery of genetic markers linked to milk production traits can be utilised to drive selection of high-performance animals.A previously reported genome wide association study across dairy goats in New Zealand identified a quantitative trait loci (QTL) located on chromosome 19. The most significantly associated single nucleotide polymorphism (SNP) marker for this locus is located at position 26,610,610 (SNP marker 19:26,610,610). This locus is associated with multiple milk production traits including fat, protein and volume. The predicted effect of selection for the beneficial haplotype would result in an average production increase of 2.2kg fat, 1.9kg protein and 73.6kg volume.An outstanding question was whether selection for the beneficial allele would co-select for any negative pleiotropic effects. An adverse relationship between milk production and udder health traits has been reported at this locus. Therefore, a genome wide association study was undertaken looking for loci associated with udder traitsResults: The QTL and production associated marker 19:26,610,610 was identified in this study to also be associated with several goat udder traits including udder depth (UD), fore udder attachment (FUA) and rear udder attachment (RUA). Our study replicates the negative relationship between production and udder traits with the high production allele at position 19:26,610,610 associated with an adverse change in UD, FUA and RUAConclusions: Our study has confirmed this negative relationship between udder traits and production traits in the NZ goat population. We have found that the frequency of the high production allele is relatively high in the NZ goat population, indicating that its effect on udder conformation is not significantly detrimental on animal health. It will however be important to monitor udder conformation as the Chromosome 19 locus is progressively implemented for marker assisted selection. It will also be of interest to determine if the gene underlying the production QTL has a direct effect on mammary gland morphology or whether the changes observed are a consequence of the increased milk volume.
Evaluation of Flexibility in Adaptation Projects for Climate Change
Authors: Kim Myung-Jin , Nicholls Robert J. , Preston John M. , De Almeida Gustavo A. M.
Published: September 2021

Climate change adaptation inherently entails investment decision-making under the high levels of uncertainty. Under these circumstances, the option of deferring a decision to adapt is one of possible strategies to address uncertainty. However, this decision will potentially leave people and areas exposed to the risk of coastal flooding during the deferral. In order to address this issue, a single fixed large investment can be divided into two or more sequential investments. This reduces the initial investment cost and adds flexibility about the size and timing of subsequent investment decisions as the magnitude of climate change becomes more available. This paper employs a real option analysis framework, as an analytical tool, to evaluate adaptations including flexibility to reduce both the risk and uncertainty of climate change, against increasing coastal flooding due to sea-level rise as an example. This paper considers (i) how to design the sequence of adaptation options under the growing risk of sea-level rise, and (ii) how to make the efficient use of flexibility included in adaptations for addressing uncertainty. This research incorporates a set of flexibilities (i.e. wait or future growth) into single-stage investments (i.e. raising coastal defence from 2.5 mAOD to 3.5mAOD or 4.0 mAOD) in two or three stages so that a set of multiple-stage adaptations are created to address both the risk and uncertainty of climate change. The proposed method compares the multiple-stage adaptations in economic terms, including optimisation, providing important additional information on the efficiency of flexible adaptation strategies given the uncertainty of climate change. The results from the analysis suggest that an efficient and robust strategy can be chosen for a short- and long- term adaptation.
Infection with a Hypervirulent Strain of Helicobacter Pylori Primes Gastric Cells Toward Intestinal Transdifferentiation
Authors: Saberi Samaneh , Esmaeili Maryam , Tashakoripour Mohammad , Hosseini Mahmoud Eshagh , Baharvand Hossein , Mohammadi Marjan
Published: September 2021

Background: Intestinal metaplasia, gastric-to-intestinal transdifferentiation, occurs as a result of the misexpression of certain regulatory factors, leading to genetic reprogramming. Here, we have evaluated the H. pylori-induced expression patterns of these candidate genes.Methods: The expression levels of 1)tissue-specific transcription factors (RUNX3, KLF5, SOX2, SALL4, CDX1 and CDX2), 2)stemness factors (TNFRSF19, LGR5, VIL1) and 3)tissue-specific mucins (MUC5AC, MUC2) were evaluated by quantitative real-time PCR in gastric primary cells (GPCs), in parallel with two gastric cancer (MKN45 and AGS) cell lines, up to 96h following H. pylori infection. Results: Following H. pylori infection of GPCs, RUNX3 declined at 24h post infection (PI) (-6.2±0.3) and remained downregulated for up to 96h. Subsequently, overexpression of self-renewal and pluripotency transcription factors, KLF5 (3.6±0.2), SOX2 (7.6±0.5) and SALL4 (4.3±0.2) occurred. The expression of TNFRSF19 and LGR5, demonstrated opposing trends, with an early rise of the former (4.5±0.3) at 8h, and a simultaneous fall of the latter (-1.8±0.5). This trend was reversed at 96h, with the decline in TNFRSF19 (-5.5±0.2), and escalation of LGR5 (2.6±0.2) and VIL1 (1.8±0.3). Ultimately, CDX1 and CDX2 were upregulated by 1.9 and 4.7-fold, respectively. The above scenario was, variably observed in MKN45 and AGS cells.Conclusion: Our data suggests an interdependent gene regulatory network, induced by H. pylori infection. This interaction begins with the downregulation of RUNX3, upregulation of self-renewal and pluripotency transcription factors, KLF5, SOX2 and SALL4, leading to the downregulation of TNFRSF19, upregulation of LGR5 and aberrant expression of intestine-specific transcription factors, potentially facilitating the process of gastric-to-intestinal transdifferentiation.
Lubricant Coatings for Mobile Dry Drilling of Stacked Aluminium Alloys
Authors: Hassan Siti Rozakiyah Assurin , Mativenga Paul T , Cooke Kevin , Sun Hailin , Field Susan , Walker Mark , Chodynicki Jason , Sharples Colin , Jensen Boris , Mortensgaard Morten F.
Published: September 2021

Aluminium alloy stack materials offer good strength-to-weight ratio and are used for high strength airframe structures. For the panels to be joined, numerous holes have to be drilled and due to structure size mobile drilling heads are used. The drilling process releases lubricant oil which becomes airborne or collects on the shop-floor and presents a health and safety hazard. Avoiding this oil release is therefore a major driver for developing dry drilling processes. However, drilling aluminium alloys in the absence of cutting fluids is a challenging task due to its tendency to adhere to the cutting tool, and the high thermal conductivity of the workpiece. Owing to their low coefficient of friction, carbon-based coatings are an option to improve the machinability of aluminium alloys. This paper presents an industrial collaboration study on the performance of carbon-based coatings in dry drilling aluminium alloy 2024 and 7150 stacks. CVD Diamond, a hard DLC, Doped MoS2 and doped amorphous carbon (Doped a-C) were evaluated in comparison with an uncoated WC drills. Coating performance was assessed in terms of tool wear, hole diameter, and surface roughness. The results revealed that CVD diamond coated drills outperformed other coatings in terms of tool wear and hole quality. The coating enabled lower aluminium pick-up on the drills as well as minimised variations in diameter deviation and hole surface roughness. The work shows the capability for dry drilling of stacked aluminium alloys and hence eliminating the health and safety risk associated with use of oil in mobile drilling heads.
LncRNA ELFN1-AS1 Upregulates TRIM29 to Promote Gastric Cancer Progression by Suppressing miR-211-3p
Authors: Huang Jinxi , Yuan Weiwei , Chen Beibei , Li Gaofeng , Chen Xiaobing
Published: September 2021

BackgroundExtracellular leucine rich repeat and fibronectin type III domain containing 1-antisense RNA 1 (ELFN1-AS1) was upregulated in tumors. Nevertheless, the biological functions of ELFN1-AS1 in gastric cancer are not fully understood.MethodsThe ELFN1-AS1, miR-211-3p and TRIM29 expression levels were determined by reverse transcription-quantitative PCR. CCK8, EDU and colony formation assays were done to test the GC cell vitality. The migratory and invasive capabilities of GC cells were further measured by transwell invasion and cell scratch assays. The ceRNA activity of ELFN1-AS1 for TRIM29 via miR-211-3pp was ascertained through pull down, RIP and luciferase reporter assays.ResultsELFN1-AS1 and TRIM29 were robustly expressed in gastric cancer tissues and negatively associated overall survival time of patients. The ELFN1-AS1 silence blocked the proliferation, migration and invasion of GC cells. The oncogenic role of ELFN1-AS1 was recognized to be modulated by miR-211-3pp, which competitively bind to 3'UTR TRIM29 and resulted in the reduced expression of TRIM29.ConclusionELFN1-AS1 maintained the tumorigensis of GC cells by ELFN1-AS1/miR-211-3pp/TRIM29 axis, suggesting that intervention targeting this axis may be warranted for GC treatment.
Understanding Reasons for Lack of Acceptance in HIV Programs Among Indigenous: a Qualitative Study in Indonesia
Authors: Sianturi Elfride Irawati , Latifah E , Soltief S N , Sihombing R , Simaremare E S , Effendy C , Probandari A N , Suryawati S , Taxis K
Published: September 2021

Background: Melanesians are Indigenous Papuans that are racially and culturally different from most Indonesians. Poverty and inequalities remain high in this region despite having the highest revenues. Furthermore, Papua has the highest HIV prevalence rate, which is 24 times higher than other regions. Therefore, this study aimed to develop an understanding of the acceptance of HIV programs among Papuans.Methods: This is a qualitative study carried out using the grounded theory with data collected from 23 Papuans through semi-structured face-to-face interviews. All interviews were conducted in Bahasa Indonesia, audio-recorded, summarized, and transcribed for analysis.Results: The results showed that to “keep being a good Papuan," emerges from 4 interconnected categories, including 1) Culture and Religion, 2) Dealing with modernisms, 3) Tailoring program and 4) Stigma reduction. As a substantive theory underpinning the experiences of indigenous people with the programs, “Keep being a good Papuan” was a way of overcoming a series of problems and dealing with modernism to eliminating HIV. This is largely focused on the local culture, therefore any adjustment needs to balance keeping their tradition and welcoming modernism.Conclusions: Therefore, the future of the programs needs to be agreed upon, funded, implemented, measured, and achieved. One of the strategies used to achieve this is by elaborating a supportive culture and community-based interventions. Further, implementation needs to be prioritized to integrate and improve the quality of the programs due to the needs of the community and the available resources.
Efficacy of Alectinib in ALK-Positive Non-Small Cell Lung Cancer with Leptomeningeal Metastases Pretreated with Crizotinib
Authors: lu zhiqin , Liang Yuanyuan , Wang Xia , Zeng Zhimin , Zhong Congying , Liu Anwen
Published: September 2021

PurposeLeptomeningeal metastases is a fatal complication of advanced non-small cell lung cancer (NSCLC). This study aimed to assess the clinical efficacy of alectinib in ALK-positive NSCLC with LM patients previously treated with crizotinib.MethodsRetrospective study of NSCLC patients with alectinib-treated ALK- positive NSCLC and LM.ResultsFourteen patients (mean age, 55 years; 50% women) with adenocarcinoma NSCLC were included in the study. Before starting alectinib, all patients were treated with crizotinib. When LM was diagnosed, all patients had clinical symptoms. Sixteen (50%) patients had a performance status>2. Of 14 total patients, 85.7% (12/14) of the patients had a clinical and radiological responses. From the start of alectinib, median OS and PFS were 17.4 (95% CI, 8.9–25.9) and 11.6 months (95% CI: 8.4–14.8), respectively, one-year survival rate was 57.1%.ConclusionAlectinib had significant efficacy against NSCLC with LM, this efficacy was rapid in several patients, even some with poor performance status. Alectinib might be a suitable option for specific patient populations with advanced ALK-positive NSCLC with LM.
Tfet Biosensor Simulation and Analysis for Various Biomolecules
Authors: Vimala P , L Likith Krishna , SS Sharma , Ainapur Rakshanda , RH Swetha
Published: September 2021

This paper investigates the simulation and performance of Tunnel field effect transistor (TFET) with a nanocavity in it, which can be used for bio sensing application. The entire simulation is done using the tool Silvaco Atlas TCAD. This paper mainly aims in comparing the different parameters for few biomolecules which has different dielectric constant values, namely Streptavidin, Biotin, APTES, Cellulose and DNA. The device structure here consists of a nanocavity near the source end, which is used to place these biomolecules and hence observe the variation of the Drain current v/s Gate voltage characteristic graph, these biomolecules that are having unique dielectric constants are placed within this cavity and these graphs are observed. The energy band diagram of this device is obtained; on top of this various other parameters namely Surface Potential, Electric field are observed for the above-mentioned Biomolecules. The Length of the cavity of the biosensor is also varied to observe the difference, in addition to this Ion (ON current) variation is plotted for the change in the dielectric constant of the biomolecule.
Plasma Proteome Changes Linked to Late Phase Response After Inhaled Allergen Challenge in Asthmatics
Authors: Weitoft Maria , Kadefors Måns , Stenberg Henning , Tufvesson Ellen , Diamant Zuzana , Enes Sara Rolandsson , Bjermer Leif , Rosmark Oskar , Westergren-Thorsson Gunilla
Published: September 2021

BackgroundA subset of individuals with allergic asthma develops a late phase response (LPR) to inhaled allergens, which is characterized by a prolonged airway obstruction, airway inflammation and airway hyperresponsiveness. The aim of this study was to identify changes in the plasma proteome and circulating hematopoietic progenitor cells associated with the LPR following an inhaled allergen challenge. MethodsSerial plasma samples from asthmatics undergoing inhaled allergen challenge were analyzed by mass spectrometry and immunosorbent assays. Peripheral blood mononuclear cells were analyzed by flow cytometry. Mass spectrometry data was analyzed using a linear regression to model the relationship between airway obstruction during the LPR and plasma proteome changes. Data from immunosorbent assays was analyzed using linear mixed models.ResultsOut of 396 proteins quantified in plasma, 150 showed a statistically significant change 23 h post allergen challenge. Among the most upregulated were three protease inhibitors: alpha-1-antitrypsin, alpha-1-antichymotrypsin and plasma serine protease inhibitor. Altered levels of 13 proteins were associated with the LPR, including increased factor XIII A and a decreased von Willebrand factor. No relationship was found between the LPR and changes in the proportions of classical, intermediate, and non-classical monocytes.Conclusions﻿Allergic reactions to inhaled allergens in asthmatic subjects was associated with changes in a large proportion of the measured plasma proteome, whereof protease inhibitors showed the largest changes, likely to influence the inflammatory response. Many of the proteins altered in relation to the LPR are associated with coagulation, highlighting potential mechanistic targets for future treatments of type-2 asthma.
Penehyclidine Hydrochloride Has the Function of Lung Protection for Acute Lung Injury in Rat Models:A Systemic Review and Meta-analysis
Authors: Luo Shasha , Tan Hongbao , Chen Li , Wang Dongwei , Hua Rong
Published: September 2021

ObjectiveThis systematic review and meta-analysis aims to review the effects of penehyclidine hydrochloride (PHC) on acute lung injury in animal models. MethodsPubMed, Embase, CNKI were searched for collecting the randomized controlled trials(RCTs) on the effects of penehyclidine hydrochloride on acute lung injury in rat models from inception to July 1, 2021. We used Cochrane evaluating quality and RevMan 5.3 software performing Meta-analysis.(lung W/D ratio、PaO2/FiO2、SOD、MPO activity、IL-6 and TNF-α) ResultsOur search including 11 studies in 3 databases. Meta-analysis showed that, Compared with the acute lung injury model group, the PHC treatment group: lung W/D ratio[MD=-1.29，95%CI（-1.94，-0.64），P＜0.01]、PaO2/FiO2[MD=1.73，95%CI（0.01，3.45），P＜0.01]、SOD[MD=7.05，95%CI（5.58，8.52），P＜0.000,01]、IL-6 [MD=-1.03，95%CI（-1.29，-0.77），P＜0.0001]、IL-6 [MD=-3.35，95%CI（-5.29，-1.40），P＜0.001]and TNF-α[MD=-2.17，95%CI（-3.27，-1.07），P＜0.0001].Conclusions﻿penehyclidine hydrochloride has the function of lung protection in acute lung injury and provides a new therapy for future clinical treatment.
Chloroplast Genome Analysis of The Famous Chinese Herbal Medicine Yizhiren (Zingiberaceae)
Authors: Pan JiaoYi , Shiyun Sun , Qiong Xia , Xvhan Lv , Xinxian Yang , Fule He
Published: September 2021

Background: Yizhiren is the fruit of Alpinia oxyphylla(Zingiberaceae) ,a well-known Chinese herbal medicine from China. The complete chloroplast genome of Alpinia oxyphylla was studied in this paper, which laid the foundation of the further study of genetic information and data of Alpinia oxyphylla.Methods: The complete chloroplast sequences of 19 the family Zingiberaceae species were aligned using MEGAX software.The phylogenetic tree was constructed by the Maximum-Likelihood method and edited by the Evolview online.Results: The chloroplast gene group is a typical tetragonal structure, which is formed by 161,351 base pairs. Each genome has a large single-copy region (LSC) of 87,248 bp, a small single-copy region (SSC) of 16,175 bp and a pair of inverted-repeat regions (IRs) of 28,964 bp in each. The complete nucleotide composition of chloroplast genome is: 31.5% A, 32.4% T, 18.2% C, 17.9% G, and the total GC content is 36.2%. Among them 28 exons and 15 introns. A total of 137 genes were annotated, which included 92 protein coding genes (PCGs), 37 metastatic RNA (tRNAs) and 8 ribosomal RNA (rRNAs).Conclusions: The phylogenetic ML tree shown the conclusion that Alpinia oxyphylla is closely related to Alpinia chinensis on genetic position relationship. This result is of great value to the study of biological inheritance, species identification and medicinal value. Meanwhile, it provides references for the study of biological inheritance, species identification and medicinal value.
Circulating Carnitine Levels and Breast Cancer: A Matched Case-Control Study
Authors: Zhang Jiayi , Wu Gang , Zhu Hailong , Yang Fengyuan , Yang Shuman , Vuong Ann M , Li Jincheng , Zhu Demiao , Tao Wei
Published: September 2021

Background: The existing epidemiologic studies on the association between carnitine and breast cancer development are scarce. This study examined the association between circulating carnitine levels and breast cancer in females.Methods: This 1:1 age-matched case-control study identified 991 female breast cancer cases and 991 female controls without breast cancer. All cases and controls were confirmed with a pathological test. We measured 16 types of whole blood carnitine levels, such as free carnitine (C0) and octadecanoylcarnitine (C18), using targeted metabolomic technology. Results: The average age for cases and controls were 50.0 years (SD: 8.7 years) and 49.5 years (SD: 8.7 years), respectively. After adjusting for covariates, each SD increase in malonylcarnitine (C3DC; OR 0.91; 95% CI 0.83-1.00), decenoylcarnitine (C10:1; OR 0.87; 95% CI 0.79-0.96) and decadienoylcarnitine (C10:2; OR 0.90; 95% CI 0.82-0.99) level was associated with decreased odds of breast cancer. However, higher butyrylcarnitine (C4) levels were associated with increased risk of breast cancer (OR 1.12; 95% CI 1.02-1.23). We observed no relationship between other carnitines with breast cancer. The false discovery rates for C3DC, C4, C10:1 and C10:2 were 0.172, 0.120, 0.064 and 0.139, respectively. Conclusions: Higher levels of C3DC, C10:1, and C10:2 were protective factors for breast cancer, whereas increased C4 levels were a risk factor for breast cancer.
Mondor's Disease of Chest Wall: A Case Report
Authors: Zhang Kai , Xu Jiannan , Wu Yonghui , Wu Weibin , Zhang Jian
Published: September 2021

Background: Mondor's disease (MD) is a rare benign and self-limited entity characterized by superficial thrombophlebitis of a vein in special regions. It may be secondary to trauma, malignancy, surgery, excessive physical activity or hyperviscosity. Diagnosis of MD is often based on history and physical examination. Case presentation: Here we present a rare case of Mondor's disease of chest wall with a pain and subcutaneous cord-like induration of right lateral chest. Ultrasonography revealed a superficial venous thrombosis of right thoracoepigastric vein. After treatment of aspirin for 2 weeks, chest pain was obviously relieved, and the lesion completely resolved approximately 6 weeks after presentation. Conclusions: Mondor’s disease could spontaneously resolved after several weeks and excessive medical intervention should be avoided.
Ge-Source Based L-Shaped Tunnel Field Effect Transistor for Low Power Switching Application
Authors: Chander Sweta , Sinha Sanjeet Kumar , Chaudhary Rekha , Singh Avtar
Published: September 2021

In this work, the performance of the heterojunction L-Tunnel Field Effect Transistor (LTFET) has been analyzed with different engineering techniques such as bandgap engineering, pocket engineering, work-function engineering, and gate dielectric engineering, respectively. The electrical characteristics of the device has been investigated by using Synopsys Sentaurus TCAD tool and compared with some recent other TFETs. The proposed Ge-source L-TFET device with n-type pocket shows ON-state current of 2.12*10-5 Aµm-1, OFF-state current of 1.09*10-13 Aµm-1, current ratio of ~108 and sub-threshold slope (SS) of 21 mV/decade and the threshold voltage of 0.26 V and compared to the conventional Si/Ge source L-shaped TFETs without pocket simulation result. The pocket engineering techniques suppress the leakage without degrading the ON current, threshold voltage and SS of the proposed device. The simplified fabrication steps of the proposed device have also been discussed. The proposed L-TFET is free from ambipolarity issues and can be used to develop low-power switching devices.
CT-based Radiomic Nomogram for Predicting the Severity of Patients With COVID-19
Authors: Shi Hengfeng , Xu Zhihua , Cheng Guohua , Ji Hongli , He Linyang , Zhu Juan , Hu Hanjin , Xie Zongyu , Ao Weiqun , Wang Jian
Published: September 2021

Background: The coronavirus disease 2019 (COVID-19) is a pandemic now, and the severe COVID-19 determines the management and treatment, even prognosis. We aim to develop and validate a radiomics nomogram for identifying severe patients with COVID-19. To develop and validate a radiomics nomogram for identifying severe patients with COVID-19.Methods: There were 156 and 104 patients with COVID-19 enrolled in primary and validation cohorts respectively. Radiomics features were extracted from chest CT images. Least absolute shrinkage and selection operator (LASSO) method was used for feature selection and radiomics signature building. Multivariable logistic regression analysis was used to develop a predictive model, and the radiomics signature, abnormal WBC counts, and comorbidity were incorporated and presented as a radiomics nomogram. The performance of the nomogram was assessed through its calibration, discrimination, and clinical usefulness.Results: The radiomics signature consisting of 4 selected features was significantly associated with clinical condition of patients with COVID-19 in the primary and validation cohorts (P<0.001). The radiomics nomogram including radiomics signature, comorbidity and abnormal WBC counts, showed good discrimination of severe COVID-19, with an AUC of 0.972, and good calibration in the primary cohort. Application of the nomogram in the validation cohort still gave good discrimination with an AUC of 0.978 and good calibration. Decision curve analysis demonstrated that the radiomics nomogram was clinically useful to identify the severe COVID-19.Conclusion: We present an easy-to-use radiomics nomogram to identify the severe patients with COVID-19 for better guiding a prompt management and treatment.