bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of lumbar decompression under large-channel spinal endoscope in elderly patients with segmental lumbar spinal stenosis
Authors: Zhang Fei , Ye Dandan , Zhang Wei , Sun Yapeng , Guo Lei , Li Jiaqi
Published: January 2024

Objective

The present study was conducted with an attempt to explore the overall efficacy of large-channel spinal endoscopy technology in elderly patients with segmental lumbar spinal stenosis.

Methods

We included a total of 68 elderly patients with segmental lumbar spinal stenosis in our hospital from February 2021 to March 2023. The participants were randomly and equally distributed into the study group and the control group using a random number table method. The control group received the open lumbar decompression surgery, and the study group received the lumbar decompression under large-channel spinal endoscopy technology. We compared the surgical conditions of the two groups, including pain level, Oswestry Disability Index (ODI) score, and Japanese Orthopedic Association (JOA) score before surgery, 1 week after surgery, 3 months after surgery, and 1 year after surgery. In addition, we compared the efficacy and adverse reactions 1 year after surgery between the two groups.

Results

Our findings revealed that the operation time, intraoperative blood loss, postoperative drainage volume, and hospital stay in the study group were significantly lower than those in the control group (p < 0.05). There was no statistically significant difference in the degree of pain between the two groups before surgery (p > 0.05), and the pain intensity of the study group was significantly lower than that of the control group at 1 week, 3 months, and 1 year after surgery (p < 0.05). Similarly, preoperative ODI and JOA scores were not significantly different between the two groups (p > 0.05), while they were significantly lower in the study group than those in the control group at 1 week, 3 months, and 1 year after surgery (p < 0.05). Before surgery, no significant difference was seen in therapeutic efficacy between the two groups (p > 0.05), whereas the efficacy was remarkably improved in the study group comparing to the control group at 1 week, 3 months, and 1 year after surgery (p < 0.05). All patients in this study were followed up for 10 to 16 months, with an average of 13.29 ± 1.28 months. The incidence of adverse reactions in the study group was significantly lower than that in the control group (p < 0.05).

Conclusions

Large-channel spinal endoscopy technology exerted promising results in elderly patients with segmental lumbar spinal stenosis, in terms of reducing the surgical time, intraoperative bleeding, postoperative drainage volume, and hospital stay. The approach also alleviated pain, reduced ODI and JOA scores, and restored lumbar function, with decreased incidence of adverse reactions, thereby promoting patient recovery. It is considered valid for wide clinical application.
Incidence and risk factors of perioperative deep vein thrombosis in patients undergoing primary hip arthroplasty via the direct anterior approach
Authors: Zhuang Zaikai , Li Qiangqiang , Yao Yao , Shen Ying , Chen Dongyang , Jiang Qing
Published: January 2024

Background

Deep vein thrombosis (DVT) is a frequent complication following hip arthroplasty. There still has been a lack of studies analyzing the perioperative risk factors of DVT following hip arthroplasty via direct anterior approach (DAA).

Methods

Patients who underwent unilateral primary hip arthroplasty via DAA in our hospital from August 2015 to January 2022 were included. Patients’ data, including demographic data, clinical features, past medical history, operative data, and laboratory data, were analyzed and compared between patients with and without DVT. Logistic regression analysis was conducted to identify the independent risk factors. Receiver operating characteristic (ROC) curve analysis was used to assess the best cutoff value of continuous variables with statistical significance.

Result

A total of 651 patients were included. The incidence of DVT before and after hip arthroplasty was 12.7% and 6.7%, respectively. Logistic regression analysis indicated that age ≥ 65 years (OR 4.594, 95% CI 1.994–10.587), women (OR 2.331, 95% CI 1.285–4.227), and cerebral infarction (OR 1.984, 95% CI 1.138–3.460) were independent risk factors for preoperative DVT. And age ≥ 65 years (OR 4.859, 95% CI 1.062–22.226), tumor (OR 3.622, 95% CI 1.108–11.841), and preoperative D-dimer (OR 1.040, 95% CI 1.004–1.078) were risk factors for postoperative DVT. The ROC curve analysis showed that the best cutoff value of preoperative D-dimer for the diagnosis of postoperative DVT is 1.44 mg/L.

Conclusions

The incidence of DVT in patients undergoing DAA hip arthroplasty was low and the occurrence of DVT before and after unilateral primary hip arthroplasty performed through DAA was related to multiple factors.
Factors associated with intervertebral cage subsidence in posterior lumbar fusion
Authors: Liu Yan , Li Nian-Hu
Published: January 2024

Background

The interbody fusion apparatus is a key component of the operation and plays a key role in the postoperative efficacy. Cage subsidence is one of the common complications after lumbar fusion and internal fixation. Clinical studies on the risk factors of cage subsidence are incomplete and inaccurate, especially paravertebral muscle atrophy and intervertebral bone fusion time.

Methods

Among the patients who underwent PLIF surgery in our hospital from January 2016 to January 2019, 30 patients with cage subsidence and 30 patients without cage subsidence were randomly selected to be included in this study. The differences between the two groups were compared, and the relevant factors of cage subsidence were explored by single factor comparison and multiple logistic regression analysis.

Results

Bone mineral density (T) of the subsidence group [(− 1.84 ± 1.81) g/cm2 vs (− 0.87 ± 1.63) g/cm2, P = 0.018] was significantly lower than that of the normal group. There were 4 patients with end plate injury in the subsidence group (P = 0.038). Preoperative end plate Modic changes [I/II/III, (7/2/2) vs (2/5/8), P = 0.043] were significantly different between the two groups. In the subsidence group, preoperative rCSA of psoas major muscle [(1.43 ± 0.40) vs (1.64 ± 0.41), P = 0.043], CSA of paravertebral muscle [(4530.25 ± 776.55) mm2 vs (4964.75 ± 888.48) mm2, P = 0.047], paravertebral muscle rCSA [(3.03 ± 0.72) vs (3.84 ± 0.73), P < 0.001] and paravertebral muscle rFCSA [(2.29 ± 0.60) vs (2.89 ± 0.66), P < 0.001] were significantly lower than those in normal group. In the subsidence group, the vertebral body area [(1547.81 ± 309.89) mm2 vs (1326.48 ± 297.21) mm2, P = 0.004], the height of the immediately corrected vertebral space [(2.86 ± 1.10) mm vs (1.65 ± 1.02) mm, P = 0.020], immediately SL corrective Angle [(5.81 + 4.71)° vs (3.24 + 3.57) °, P = 0.009), postoperative PI—LL [(11.69 + 6.99)° vs (6.66 + 9.62) °, P = 0.029] and intervertebral fusion time [(5.38 ± 1.85) months vs (4.30 ± 1.49) months, P = 0.023] were significantly higher than those in the normal group. Multivariate logistic regression analysis showed that the time of intervertebral fusion (OR = 1.158, P = 0.045), the height of immediate intervertebral space correction (OR = 1.438, P = 0.038), and the Angle of immediate SL correction (OR = 1.101, P = 0.019) were the risk factors for cage subsidence. Bone mineral density (OR = 0.544, P = 0.016) and preoperative paravertebral muscle rFCSA (OR = 0.525, P = 0.048) were protective factors.

Conclusion

Intervertebral fusion time, correctable height of intervertebral space, excessive Angle of immediate SL correction, bone mineral density and preoperative paravertebral muscle rFCSA are risk factors for cage subsidence after PLIF.
Effect of long-term cervical extensor exercise program on functional disability, pain intensity, range of motion, cervical muscle mass, and cervical curvature in young adult population with chronic non-specific neck pain: a randomized controlled trial
Authors: Zhang Yao , Lin Wancheng , Yi Meng , Song Jipeng , Ding Lixiang
Published: January 2024

Background

The prevalence of chronic non-specific neck pain (CNNP) is on the rise among the young adult population. We herein aimed to compare the effects of long-term specific cervical extensor training and stretching exercises on improving this chronic disorder in young adults.

Methods

In this prospective, randomized, controlled study, 70 participants aged 18–35 years with CNNP and cervical lordosis loss were included. The participants were assigned to undergo either specific cervical extensor training (observation group) or perform usual stretching exercises (control group). The exercise duration was set at 12 months, with 9 months at the clinic and 3 months at home. The outcome assessments included changes in the neck disability index, visual analog scale from baseline, cervical range of motion (CROM), cross-sectional areas (CSAs) of cervical extensors, and cervical curvature from baseline. The outcome measures were compared between groups at 3, 6, and 12 months of follow-up.

Results

All 70 participants underwent randomization, and no significant differences in demographics and baseline data were found between the two groups. The observation group showed a greater improvement in neck disability index and visual analog scale scores at the 12-month follow-up than the control group. Additionally, a more substantial increase in CROM and CSAs of cervical extensors was observed in the observation group at the 6-month and 12-month follow-ups (P < 0.05). Although more participants in the observation group achieved cervical lordosis at the 12-month follow-up, the difference was marginally nonsignificant (9% in the control group vs. 28% in the observation group, P = 0.075).

Conclusions

In young adults with CNNP, long-term specific cervical extensor training was associated with a more significant clinically meaningful improvement in disability, pain, and CROM than stretching exercises. The increased CSAs of cervical extensors may potentially contribute to the restoration of cervical lordosis.

Trial registration The study is registered at the Chinese domestic clinical trial (ChiCTR2000040009) at Chictr.org. The date of registration: November 18, 2020.
Fusion with and without lever reduction in degenerative lumbar spondylolisthesis: a retrospective study
Authors: Kong Chao , Wang Dongfan , Wang Wei , Wang Yu , Lu Shibao
Published: January 2024

Background

The reduction of slipped vertebra is often performed during surgery for degenerative lumbar spondylolisthesis (DLS). This approach, while potentially improving clinical and radiological outcomes, also carries a risk of increased complications due to the reduction process. To address this, we introduced an innovative lever reduction technique for DLS treatment. This study aims to investigate the clinical efficacy, radiological outcomes, and complications of fusion with or without lever reduction.

Methods

We conducted a retrospective review of prospectively collected data from a registry of patients who underwent lumbar fusion surgery for DLS, with a follow-up of at least 24 months. Self-reported measures included visual analog scale (VAS) for back or leg pain, Oswestry Disability Index (ODI), and the achievement of minimal clinically important difference (MCID). Radiological assessments encompassed spondylolisthesis percentage (SP), focal lordosis (FL), and lumbar lordosis (LL). Complications were categorized using the modified Clavien–Dindo classification (MCDC) scheme. Patients were assigned to the reduction group (RG) and non-reduction group (NRG) based on the application of the lever reduction technique. Clinical and radiological outcomes at baseline, immediately after surgery, and at the last follow-up were compared.

Results

A total of 281 patients were analyzed (123 NRG, 158 RG). Baseline patient demographics, comorbidities, and surgical characteristics were similarly distributed between groups except for operating time (NRG 129.25 min, RG 138.04 min, P = .009). Both groups exhibited significant clinical improvement after surgery (all, P = .000), with no substantial difference between groups (VAS, ODI, or the ability to reach MCID). Patients in RG showed statistically lower SP and higher FL during follow-up (all, P = .000). LL was comparable at different time points within each group or at the same time point between the two groups (all, P > .050). The overall complication rate (NRG 38.2%, RG 27.2%, P = .050) or specific complication rates per MCDC were similar between groups (all, P > .050). Patients in RG were predisposed to a lower risk of adjacent segment degeneration (ASDeg) (NRG 9.8%, RG 6.3%, P = .035).

Conclusions

There were no significant differences in postoperative measures such as VAS scores for back and leg pain, ODI, the ability to reach MCID, overall complication rate, or specific complication rates per MCDC between surgical approaches. However, fusion with lever reduction demonstrated a notable advantage in restoring segmental spinal sagittal alignment and reducing the occurrence of ASDeg compared to in situ fusion.
Network meta-analysis of invasive treatment for early-stage osteonecrosis of the femoral head
Authors: Li Yingchun , Ma Xiuying , Dong Bo , Li Yue , Liang Zhuang
Published: January 2024

Background

Osteonecrosis of the femoral head (ONFH) is a common disabling disease in orthopedics. Blocking the progression of ONFH in the early stage is essential for avoiding total hip replacement.

Purposes

The purpose of this study is to evaluate the effect of invasive treatment on early-stage ONFH.

Methods

According to the PRISMA guidelines, relevant English databases were searched in August 2022 to collect published research. Extract result indicators and conduct network meta-analysis using R software.

Results

A total of 15 RCTs were included. All patients were diagnosed with early-stage ONFH. The surface under the cumulative ranking curve (SUCRA) showed that CD + BMMSC and CD + PRP were the most effective in improving HHS. The results of the league table showed that CD + BMMSC was superior to CD alone. Meanwhile, the SUCRA for FR showed that CD + BG + BMMSC was the most likely to be the most effective in reducing FR. The league table revealed that CD + BG, CD + BG + BMMSC, and CD + BMMSC were superior to CD alone, with statistically significant differences.

Conclusion

Considering the HHS and FR, CD + BMMSC may be the optimal treatment option to effectively delay the progression of ONFH and restore the postoperative function of patients.

Registration Number: The study protocol has been registered on the PROSPERO platform (CRD42023380169).
Research progress in the management of vascular disease with cannabidiol: a review
Authors: Guo Yilong , Wei Ren , Deng Jianqing , Guo Wei
Published: January 2024

The morbidity and mortality rates associated with vascular disease (VD) have been gradually increasing. Currently, the most common treatment for VD is surgery, with the progress in drug therapy remaining slow. Cannabidiol (CBD) is a natural extract of Cannabis sativa L. with sedative, analgesic, and nonaddictive properties. CBD binds to 56 cardiovascular-related receptors and exerts extensive regulatory effects on the cardiovascular system, making it a potential pharmacological agent for the management of VD. However, most CBD studies have focused on neurological and cardiac diseases, and research on the management of VD with CBD is still rare. In this review, we summarize the currently available data on CBD in the management of VD, addressing four aspects: the major molecular targets of CBD in VD management, pharmacokinetic properties, therapeutic effects of CBD on common VDs, and side effects. The findings indicate that CBD has anti-anxiety, anti-oxidation, and anti-inflammatory properties and can inhibit abnormal proliferation and apoptosis of vascular smooth muscle and endothelial cells; these effects suggest CBD as a therapeutic agent for atherosclerosis, stress-induced hypertension, diabetes-related vasculopathy, ischemia-reperfusion injury, and vascular damage caused by smoking and alcohol abuse. This study provides a theoretical basis for further research on CBD in the management of VD.
Forest carbon stock development following extreme drought-induced dieback of coniferous stands in Central Europe: a CBM-CFS3 model application
Authors: Cienciala Emil , Melichar Jan
Published: January 2024

Background

We analyze the forest carbon stock development following the recent historically unprecedented dieback of coniferous stands in the Czech Republic. The drought-induced bark-beetle infestation resulted in record-high sanitary logging and total harvest more than doubled from the previous period. It turned Czech forestry from a long-term carbon sink offsetting about 6% of the country's greenhouse gas emissions since 1990 to a significant source of CO2 emissions in recent years (2018–2021). In 2020, the forestry sector contributed nearly 10% to the country's overall GHG emissions. Using the nationally calibrated Carbon Budget Model of the Canadian Forest Sector (CBM-CFS3) at a regional (NUTS3) spatial resolution, we analyzed four scenarios of forest carbon stock development until 2070. Two critical points arise: the short-term prognosis for reducing current emissions from forestry and the implementation of adaptive forest management focused on tree species change and sustained carbon accumulation.

Results

This study used four different spruce forest dieback scenarios to assess the impact of adaptive forest management on the forest carbon stock change and CO2 emissions, tree species composition, harvest possibilities, and forest structure in response to the recent unprecedented calamitous dieback in the Czech Republic. The model analysis indicates that Czech forestry may stabilize by 2025 Subsequently, it may become a sustained sink of about 3 Mt CO2 eq./year (excluding the contribution of harvested wood products), while enhancing forest resilience by the gradual implementation of adaptation measures. The speed of adaptation is linked to harvest intensity and severity of the current calamity. Under the pessimistic Black scenario, the proportion of spruce stands declines from the current 43–20% by 2070, in favor of more suited tree species such as fir and broadleaves. These species would also constitute over 50% of the harvest potential, increasingly contributing to harvest levels like those generated by Czech forestry prior to the current calamity. The standing stock would only be recovered in 50 years under the optimistic Green scenario.

Conclusion

The results show progress of adaptive management by implementing tree species change and quantify the expected harvest and mitigation potential in Czech forestry until 2070.
Health systems resilience in fragile and conflict-affected settings: a systematic scoping review
Authors: Truppa Claudia , Yaacoub Sally , Valente Martina , Celentano Giulia , Ragazzoni Luca , Saulnier Dell
Published: January 2024

Background

Health systems resilience (HSR) research is a rapidly expanding field, in which key concepts are discussed and theoretical frameworks are emerging with vibrant debate. Fragile and conflict-affected settings (FCAS) are contexts exposed to compounding stressors, for which resilience is an important characteristic. However, only limited evidence has been generated in such settings. We conducted a scoping review to: (a) identify the conceptual frameworks of HSR used in the analysis of shocks and stressors in FCAS; (b) describe the representation of different actors involved in health care governance and service provision in these settings; and (c) identify health systems operations as they relate to absorption, adaptation, and transformation in FCAS.

Methods

We used standard, extensive search methods. The search captured studies published between 2006 and January 2022. We included all peer reviewed and grey literature that adopted a HSR lens in the analysis of health responses to crises. Thematic analysis using both inductive and deductive approaches was conducted, adopting frameworks related to resilience characteristics identified by Kruk et al., and the resilience capacities described by Blanchet et al.

Results

Thirty-seven studies met our inclusion criteria. The governance-centred, capacity-oriented framework for HSR emerged as the most frequently used lens of analysis to describe the health responses to conflict and chronic violence specifically. Most studies focused on public health systems’ resilience analysis, while the private health sector is only examined in complementarity with the former. Communities are minimally represented, despite their widely acknowledged role in supporting HSR. The documentation of operations enacting HSR in FCAS is focused on absorption and adaptation, while transformation is seldom described. Absorptive, adaptive, and transformative interventions are described across seven different domains: safety and security, society, health system governance, stocks and supplies, built environment, health care workforce, and health care services.

Conclusions

Our review findings suggest that the governance-centred framework can be useful to better understand HSR in FCAS. Future HSR research should document adaptive and transformative strategies that advance HSR, particularly in relation to actions intended to promote the safety and security of health systems, the built environment for health, and the adoption of a social justice lens.
Radiotherapy-activated NBTXR3 nanoparticles promote ferroptosis through induction of lysosomal membrane permeabilization
Authors: Da Silva Jordan , Bienassis Célia , Schmitt Peter , Berjaud Céline , Guedj Mickael , Paris Sébastien
Published: January 2024

Purpose

Radiotherapy-activated NBTXR3 (NBTXR3 + RT) has demonstrated superior efficacy in cancer cell destruction and tumor growth control, compared to radiotherapy (RT), in preclinical and clinical settings. Previous studies highlighted the immunomodulatory properties of NBTXR3 + RT, such as modification of tumor cell immunogenicity/adjuvanticity, producing an effective local tumor control and abscopal effect, related to an enhanced antitumor immune response. Furthermore, NBTXR3 + RT has shown potential in restoring anti-PD1 efficacy in a refractory tumor model. However, the early events leading to these results, such as NBTXR3 endocytosis, intracellular trafficking and primary biological responses induced by NBTXR3 + RT remain poorly understood.

Methods

We analyzed by transmission electron microscopy endocytosis and intracellular localization of NBTXR3 nanoparticles after endocytosis in various cell lines, in vitro and in vivo. A kinetic of NBTXR3 endocytosis and its impact on lysosomes was conducted using LysoTracker staining, and a RNAseq analysis was performed. We investigated the ability of NBTXR3 + RT to induce lysosomal membrane permeabilization (LMP) and ferroptosis by analyzing lipid peroxidation. Additionally, we evaluated the recapture by cancer cells of NBTXR3 released from dead cells.

Results

NBTXR3 nanoparticles were rapidly internalized by cells mainly through macropinocytosis and in a less extend by clathrin-dependent endocytosis. NBTXR3-containing endosomes were then fused with lysosomes. The day following NBTXR3 addition, we measured a significant increase in LysoTracker lysosome labeling intensity, in vitro as in vivo. Following RT, a significant lysosomal membrane permeabilization (LMP) was measured exclusively in cells treated with NBTXR3 + RT, while RT had no effect. The day post-irradiation, a significant increase in lipid peroxidation, a biomarker of ferroptosis, was measured with NBTXR3 + RT compared to RT. Moreover, we demonstrated that NBTXR3 nanoparticles released from dead cells can be recaptured by cancer cells.

Conclusions

Our findings provide novel insights into the early and specific biological effects induced by NBTXR3 + RT, especially LMP, not induced by RT in our models. The subsequent significant increase in lipid peroxidation partially explains the enhanced cancer cell killing capacity of NBTXR3 + RT compared to RT, potentially by promoting ferroptosis. This study improves our understanding of the cellular mechanisms underlying NBTXR3 + RT and highlights its potential as an agnostic therapeutic strategy for solid cancers treatment.

Graphical Abstract
Efficacy and Safety of Once-Weekly Insulin Regimes on Glycemic Control for Type 2 Diabetes: A Systematic Review and Network Meta-analysis
Authors: Wang Peng , Zhang Yu , Xu Wenhao , He Jialing , Peng Liyuan , Feng Yuning , Xu Ping , Chong Weelic , Hai Yang , Jia Lu , Fang Fang
Published: January 2024

Background

Randomized controlled trials have found that once-weekly insulin resulted in greater glycemic control compared to once-daily insulin in patients with type 2 diabetes. However, no direct comparisons have been made between different types of once-weekly insulin thus far. This systematic review and network meta-analysis aimed to evaluate the effect of the two most advanced once-weekly insulin analogues, namely insulin icodec and insulin Fc, in patients with type 2 diabetes.

Methods

We conducted a thorough search in the databases PubMed, Embase, and the Cochrane Central Register of Controlled Trials. The search included articles published from the beginning to October 10, 2023, with no language limitations. Our aim was to conduct a systematic review of randomized controlled trials that investigated the effectiveness and safety of once-weekly insulin in individuals with type 2 diabetes. Our primary outcome was to evaluate excellent glycemic control, defined as patients achieving glycated hemoglobin levels below 7%.

Results

We identified a total of 7 trials involving 2829 patients. The results showed that once-weekly insulin icodec is more effective than once-weekly insulin Fc (RR 1.59 [95% CI 1.08–2.38]), once-daily degludec (RR 1.43 [95% CI 1.14–1.83]), and once-daily glargine (RR 1.15 [95% CI 1.00-1.41]). Moreover, the incidence of severe hypoglycemia was lower with once-weekly insulin icodec compared to once-daily degludec (RR 0.00016 [95% CI 0 to 0.41]). However, no significant difference in the incidence of severe hypoglycemia was observed between once-weekly insulin icodec and once-daily glargine (RR 0.39 [95% CI 0.03 to 4.83]).

Conclusions

In patients with type 2 diabetes, once-weekly insulin icodec achieved superior glycemic control compared to once-weekly insulin Fc, with no significant difference in the occurrence of hypoglycemia. The ranking probability results have shown that one weekly icodec seems to be the preferred option in patients with type 2 diabetes.

Trial registration

PROSPERO Identifier: CRD42023470894.
Reliability of mixed dentition space analysis using a digital model obtained from an optical impression: a preliminary study
Authors: Okamoto Ayuko , Karibe Hiroyuki , Tanaka Satoshi , Kawakami Tomomi , Shinya Akikazu
Published: January 2024

Objective

While mixed dentition space analysis is a common practice in pediatric dentistry, digital models created using an intraoral scanner are not as widely used in clinical settings. This preliminary study used a very small sample size with one reference model and aimed to (1) compare the accuracy of mixed dentition space analysis using a digital model obtained from an optical impression with that of conventional plaster model-based analysis and (2) assess inter-examiner differences.

Results

The space required for the mandibular permanent canine and premolars and arch length discrepancy were calculated using each model. The largest significant difference between plaster- and digital model-based analyses was identified when the right arch length discrepancy was considered (-0.49 mm; 95% confidence interval: -0.95–0.03); however, the value was considered clinically insignificant. Significant inter-examiner differences were observed for six items of the plaster model; however, no such differences were observed when using the digital model. In conclusion, digital model space analysis may have the same level of accuracy as conventional plaster model analysis and likely results in smaller inter-examiner differences than plaster model analysis.
Non-coding RNA in the gut of the blood-feeding parasitic worm, Haemonchus contortus
Authors: Zhou Caixian , Tuersong Waresi , Liu Lu , Di Wenda , He Li , Li Fangfang , Wang Chunqun , Hu Min
Published: January 2024

The intestine of Haemonchus contortus is an essential tissue that has been indicated to be a major target for the prevention of haemonchosis caused by this parasitic nematode of small ruminants. Biological peculiarities of the intestine warrant in-depth exploitation, which can be leveraged for future disease control efforts. Here, we determined the intestinal ncRNA (lncRNA, circRNA and miRNA) atlas using whole-transcriptome sequencing and bioinformatics approaches. In total, 4846 novel lncRNA, 982 circRNA, 96 miRNA (65 known and 31 novel) and 8821 mRNA were identified from the H. contortus intestine. The features of lncRNA, circRNA and miRNA were fully characterized. Comparison of miRNA from the intestines and extracellular vesicles supported the speculation that the miRNA from the latter were of intestinal origin in H. contortus. Further function analysis suggests that the cis-lncRNA targeted genes were involved in protein binding, intracellular anatomical structure, organelle and cellular process, whereas the circRNA parental genes were mainly enriched in molecular function categories, such as ribonucleotide binding, nucleotide binding, ATP binding and carbohydrate derivative binding. The miRNA target genes were related to the cellular process, cellular response to stimulus, cellular protein modification process and signal transduction. Moreover, competing endogenous RNA network analysis revealed that the majority of lncRNA, circRNA and mRNA only have one or two binding sites with specific miRNA. Lastly, randomly selected circRNA, lncRNA and miRNA were verified successfully using RT-PCR. Collectively, these data provide the most comprehensive compilation of intestinal transcripts and their functions, and it will be helpful to decipher the biological and molecular complexity of the intestine and lay the foundation for further functional research.
Design of an oral vaccine using Lactococcus lactis against brucellosis: an in vitro and in vivo study
Authors: Kazemi-Roudsari Mahsa , Doosti Abbas , Jami Mohammad-Saeid
Published: January 2024

Brucellosis is regarded as one of the world’s most severe zoonotic diseases. This study aimed to investigate the possibility of using recombinant Lactococcus lactis (L. lactis) as a live vector to produce recombinant Brucella abortus (B. abortus) Omp10. The gene sequences were obtained from GenBank. The proteins’ immunogenicity was assessed using Vaxijen. After confirming the cloning of the Omp10 gene in the pNZ8148 vector by enzymatic digestion and PCR, transformation into L. lactis was done. SDS-PAGE and western blot methods evaluated omp10 protein expression. Mice received oral recombinant L. lactis vaccines. IgG antibodies against Omp10 were tested using ELISA. Real-time PCR and ELISA were used to analyze cytokine responses. Survival rate and histopathological changes were evaluated after the challenge. Omp10 was chosen for its 1.5524 antigenicity score. Enzymatic digestion and PCR identified a 381-bp gene fragment. A 10 kDa band indicated the success of L. lactis transformation. Mice administered the L. lactis-pNZ8148-Omp10-Usp45 vaccination 14 days after priming showed significantly higher Omp10-specific total IgG and IgG1 (P < 0.001) than the PBS control group. The mice who received the L. lactis-pNZ8148-Omp10-Usp45 and IRBA vaccines had significantly elevated levels of IFN-γ, TNFα, IL-4, and IL-10 in samples collected on days 14 and 28 (P < 0.001). Inflammatory response, morphological damage, alveolar edema, and lymphocyte infiltration were reduced in the target group. A recombinant L. lactis expressing the Omp10 protein was constructed as an oral Lactococcus-based vaccine and compared to live attenuated vaccines for future brucellosis investigations.
Current progression in application of extracellular vesicles in central nervous system diseases
Authors: Zhang Xiang-Min , Huang Jie , Ni Xiao-Ying , Zhu Hui-Ru , Huang Zhong-Xin , Ding Shuang , Yang Xin-Yi , Tan Yan-Di , Chen Jian-Fu , Cai Jin-Hua
Published: January 2024

Early diagnosis and pharmacological treatment of central nervous system (CNS) diseases has been a long-standing challenge for clinical research due to the presence of the blood–brain barrier. Specific proteins and RNAs in brain-derived extracellular vesicles (EVs) usually reflect the corresponding state of brain disease, and therefore, EVs can be used as diagnostic biomarkers for CNS diseases. In addition, EVs can be engineered and fused to target cells for delivery of cargo, demonstrating the great potential of EVs as a nanocarrier platform. We review the progress of EVs as markers and drug carriers in the diagnosis and treatment of neurological diseases. The main areas include visual imaging, biomarker diagnosis and drug loading therapy for different types of CNS diseases. It is hoped that increased knowledge of EVs will facilitate their clinical translation in CNS diseases.
Cell death of alveolar lymphocytes and monocytes is negatively correlated with driving pressure and mechanical power in patients with acute respiratory distress syndrome
Authors: Leu Shaw-Woei , Chu Chien-Min , Chung Chia-Jung , Huang Chih-Yu , Wang Chao-Hung , Li Li-Fu , Wu Huang-Pin
Published: January 2024

Background

Pathogenesis of acute respiratory distress syndrome (ARDS) involves immune cell death and removal from the injured lungs. ARDS severity is related to lung compliance. However, the correlation between the respiratory mechanics and alveolar immune cell death in patients with ARDS remains unclear.

Methods

Twenty-four patients with respiratory failure and ARDS were enrolled in the intensive care unit between November 2019 and November 2021. Neutrophil extracellular traps (NETs) and cell death of lymphocytes and monocytes in bronchoalveolar lavage fluid were detected on days 1 and 8.

Results

Lung compliance was positively correlated with the cell death percentage of alveolar CD4/CD8 lymphocytes and monocytes on day 8 (Pearson’s correlation coefficient (r) = 0.554, p = 0.005; r = 0.422, p = 0.040; r = 0.569, p = 0.004, respectively). There was no association between lung compliance and the percentage of alveolar NETs on days 1 and 8. The cell death percentages of alveolar CD4/CD8 lymphocytes and monocytes were negatively correlated with driving pressure (DP) on days 1 (r = − 0.440, p = 0.032; r = − 0.613, p = 0.001; r = -0.557, p = 0.005, respectively) and 8 (r = − 0.459, p = 0.024; r = − 0.407, p = 0.048; r = − 0.607, p = 0.002, respectively). The cell death percentages of alveolar CD4/CD8 lymphocytes and monocytes were also negatively correlated with mechanical power (MP) on days 1 (r = − 0.558, p = 0.005; r = − 0.593, p = 0.002; r = − 0.571, p = 0.004, respectively) and 8 (r = − 0.539, p = 0.007; r = − 0.338, p = 0.107; r = − 0.649, p < 0.001, respectively). The percentage of alveolar NETs on days 1 and 8 was not associated with DP or MP.

Conclusion

Patients with higher cell death rates of alveolar CD4/CD8 lymphocytes and monocytes exhibited lower DP and MP. Patients with less cell death of alveolar CD4/CD8 lymphocytes and monocytes required more DP or MP to maintain adequate ventilation.
Electrohydrodynamic (EHD) printing of nanomaterial composite inks and their applications
Authors: Ul Hassan Rizwan , Sharipov Mirkomil , Ryu WonHyoung
Published: January 2024

The utilization of high-resolution printed flexible electronic devices is prevalent in various fields, including energy storage, intelligent healthcare monitoring, soft robotics, and intelligent human–machine interaction, owing to its compact nature and mechanical flexibility. The EHD jet printing technology has the potential to develop the field of printing industry through its ability to fabricate high-resolution, flexible, stretchable, and 3D structures for electronic applications such as displays, sensors, and transistors. The EHD jet printing technology involves the use of solution-based inks made of diverse functional materials to print a wide range of structures. Consequently, it is imperative to have a comprehensive understanding of nanomaterial composites that are printed using EHD jet printing technology. This review provides a thorough overview of nanomaterial composite inks printed for electronic devices using EHD jet printing technology. In particular, a comprehensive overview has been provided about the utilization of EHD jet printing for nanomaterial composites in several domains, including flexible electrodes, flexible displays, transistors, energy harvesting, sensors, and biomedical applications. Moreover, this analysis presents a concise overview of the limitations and prospective future directions for nanomaterial composites fabricated by EHD jet printing.
Data reduction techniques for highly imbalanced medicare Big Data
Authors: Hancock John T. , Wang Huanjing , Khoshgoftaar Taghi M. , Liang Qianxin
Published: January 2024

In the domain of Medicare insurance fraud detection, handling imbalanced Big Data and high dimensionality remains a significant challenge. This study assesses the combined efficacy of two data reduction techniques: Random Undersampling (RUS), and a novel ensemble supervised feature selection method. The techniques are applied to optimize Machine Learning models for fraud identification in the classification of highly imbalanced Big Medicare Data. Utilizing two datasets from The Centers for Medicare & Medicaid Services (CMS) labeled by the List of Excluded Individuals/Entities (LEIE), our principal contribution lies in empirically demonstrating that data reduction techniques applied to these datasets significantly improves classification performance. The study employs a systematic experimental design to investigate various scenarios, ranging from using each technique in isolation to employing them in combination. The results indicate that a synergistic application of both techniques outperforms models that utilize all available features and data. Moreover, reduction in the number of features leads to more explainable models. Given the enormous financial implications of Medicare fraud, our findings not only offer computational advantages but also significantly enhance the effectiveness of fraud detection systems, thereby having the potential to improve healthcare services.
Feasibility of 177Lu activity quantification using a small portable CZT-based gamma-camera
Authors: Roth Daniel , Larsson Erik , Strand Joanna , Ljungberg Michael , Sjögreen Gleisner Katarina
Published: January 2024

Background

In image processing for activity quantification, the end goal is to produce a metric that is independent of the measurement geometry. Photon attenuation needs to be accounted for and can be accomplished utilizing spectral information, avoiding the need of additional image acquisitions. The aim of this work is to investigate the feasibility of 177Lu activity quantification with a small CZT-based hand-held gamma-camera, using such an attenuation correction method.

Methods

A previously presented dual photopeak method, based on the differential attenuation for two photon energies, is adapted for the three photopeaks at 55 keV, 113 keV, and 208 keV for 177Lu. The measurement model describes the count rates in each energy window as a function of source depth and activity, accounting for distance-dependent system sensitivity, attenuation, and build-up. Parameter values are estimated from characterizing measurements, and the source depth and activity are obtained by minimizing the difference between measured and modelled count rates. The method is applied and evaluated in phantom measurements, in a clinical setting for superficial lesions in two patients, and in a pre-clinical setting for one human tumour xenograft. Evaluation is made for a LEHR and an MEGP collimator.

Results

For phantom measurements at clinically relevant depths, the average (and standard deviation) in activity errors are 17% ± 9.6% (LEHR) and 2.9% ± 3.6% (MEGP). For patient measurements, deviations from activity estimates from planar images from a full-sized gamma-camera are 0% ± 21% (LEHR) and 16% ± 18% (MEGP). For mouse measurements, average deviations of − 16% (LEHR) and − 6% (MEGP) are obtained when compared to a small-animal SPECT/CT system. The MEGP collimator appears to be better suited for activity quantification, yielding a smaller variability in activity estimates, whereas the LEHR results are more severely affected by septal penetration.

Conclusions

Activity quantification for 177Lu using the hand-held camera is found to be feasible. The readily available nature of the hand-held camera may enable more frequent activity quantification in e.g., superficial structures in patients or in the pre-clinical setting.
Physical Activity Participation Decreases the Risk of Depression in Older Adults: The ATHLOS Population-Based Cohort Study
Authors: Lima Rodrigo A. , Condominas Elena , Sanchez-Niubo Albert , Olaya Beatriz , Koyanagi Ai , de Miquel Carlota , Haro Josep Maria
Published: January 2024

Background

To which extent physical activity is associated with depression independent of older adults’ physical and cognitive functioning is largely unknown. This cohort study using harmonised data by the EU Ageing Trajectories of Health: Longitudinal Opportunities and Synergies consortium, including over 20 countries, to evaluate the longitudinal association of physical activity (light-to-moderate or vigorous intensity) with depression in older adults (aged ≥ 50 years).

Results

We evaluated 56,818 participants (light-to-moderate models; 52.7% females, age 50–102 years) and 62,656 participants (vigorous models; 52.7% females, age 50–105 years). Compared to never, light-to-moderate or vigorous physical activity was associated with a lower incidence rate ratio (IRR) of depression (light-to-moderate model: once/week: 0.632, 95% CI 0.602–0.663; twice or more/week: 0.488, 95% CI 0.468–0.510; vigorous model: once/week: 0.652, 95% CI 0.623–0.683; twice or more/week: 0.591, 95% CI 0.566–0.616). Physical activity remained associated with depression after adjustment for the healthy ageing scale, which is a scale that incorporated 41 items of physical and cognitive functioning (light-to-moderate model: once/week: 0.787, 95% CI 0.752–0.824; twice or more/week: 0.711, 95% CI 0.682–0.742; vigorous model: once/week: 0.828, 95% CI 0.792–0.866; twice or more/week: 0.820, 95% CI 0.786–0.856).

Conclusions

Physical activity, of any intensity and weekly frequency, was a strong protective factor against depression, independent of physical and mental functioning. Health policies could stimulate the incorporation of lower physical activity intensity to protect against depression, which might be more feasible at the population level.
Optimal tracking control for underactuated airship
Authors: Atyya Mohamed , ElBayoumi Gamal M. , Lotfy Mohamed
Published: January 2024

A non-linear mathematical model of underactuated airship is derived in this paper based on Euler-Newton approach. The model is linearized with small disturbance theory, producing a linear time varying (LTV) model. The LTV model is verified by comparing its output response with the result of the nonlinear model for a given input signal. The verified LTV model is used in designing the LQT controller. The controller is designed to minimize the error between the output and required states response with acceptable control signals using a weighted cost function. Two LQT controllers are presented in this work based on two different costates transformations used in solving the differential Riccati equation (DRE). The first proposed assumption of costates transformation has a good tracking performance, but it is sensitive to the change of trajectory profile, whereas the second one overcomes this problem due to considering the trajectory dynamics. Therefore, the first assumption is performed across the whole trajectory tracking except for parts of trajectory profile changes where the second assumption is applied. The hybrid LQT controller is used and tested on circular, helical, and bowed trajectories. The simulation assured that the introduced hybrid controller results in improving airship performance.
A new QoS-aware service discovery technique in the Internet of Things using whale optimization and genetic algorithms
Authors: Liu Xiao , Deng Yun
Published: January 2024

Rapid technological advances have made daily life easier and more convenient in recent years. As an emerging technology, the Internet of Things (IoT) facilitates interactions between physical devices. With the advent of sensors and features on everyday items, they have become intelligent entities able to perform multiple functions as services. IoT enables routine activities to become more intelligent, deeper communication, and processes more efficient. In the dynamic landscape of the IoT, effective service discovery is key to optimizing user experiences. A Quality of Service (QoS)-aware service discovery technique is proposed in this paper to address this challenge. Through whale optimization and genetic algorithms, our method aims to streamline decision-making processes in IoT service selection. The bio-inspired optimization techniques employed in our approach facilitate the discovery of services more efficiently than traditional methods. Our results demonstrate superior performance regarding reduced data access time, optimized energy utilization, and cost-effectiveness through comprehensive simulations.
Insights on electromagnetic field distribution due to a vertical electric dipole source inside an infinite steel casing
Authors: Cuevas Nestor H.
Published: January 2024

Energized vertical steel casings have been developed to excite and monitor changes in the electrical properties of resistivity anomalies associated with hydrocarbon production and [Formula: see text] injection, among other applications. Accordingly, the spatial distribution of the fields expected in the medium surrounding a casing source has received considerable attention. However, investigations of the in-borehole distribution of the fields, currents, and charges have been lacking. Such an analysis is the objective of this work. Inside the borehole, the electric field is dominantly vertical, owing to the azimuthal symmetry of the charge density induced at the inner and outer casing boundaries. At the inner surface, charges accumulate dominantly in the vicinity of the source. At the outer surface, charges distribute along the extent of the casing, driven by the vertical channeling and radial leakage of the current flow. Inductive effects arise as the source oscillates in time, effectively resulting in a vanishing flux of azimuthal magnetic field and switching of the sign of the charge density along the vertical extent of the pipe. The alternating charge distribution yields fields propagating inward and outward within the metallic medium, describing inhomogeneous Zenneck surface waves that can interfere as they propagate and superimpose up and down the borehole. In the surrounding medium, the azimuthal magnetic field is driven by the current flowing vertically along the borehole, whereas a dominantly radial electric field is driven by the charge distributed on the outer casing surface.