bluuebunny/crossref_metadata_2025 huggingface dataset:
Neural Stem Cells/Neuronal Precursor Cells and Postmitotic Neuroblasts in Constitutive Neurogenesis and After ,Traumatic Injury to the Mesencephalic Tegmentum of Juvenile Chum Salmon, Oncorhynchus keta
Authors: Pushchina Evgeniya V. , Kapustyanov Ilya A. , Varaksin Anatoly A.
Published: January 2020

The proliferation of neural stem cells (NSCs)/neuronal precursor cells (NPCs) and the occurrence of postmitotic neuroblasts in the mesencephalic tegmentum of intact juvenile chum salmon, Oncorhynchus keta, and at 3 days after a tegmental injury, were studied by immunohistochemical labeling. BrdU+ constitutive progenitor cells located both in the periventricular matrix zone and in deeper subventricular and parenchymal layers of the brain are revealed in the tegmentum of juvenile chum salmon. As a result of traumatic damage to the tegmentum, the proliferation of resident progenitor cells of the neuroepithelial type increases. Nestin-positive and vimentin-positive NPCs and granules located in the periventricular and subventricular matrix zones, as well as in the parenchymal regions of the tegmentum, are revealed in the mesencephalic tegmentum of juvenile chum salmon, which indicates a high level of constructive metabolism and constitutive neurogenesis. The expression of vimentin and nestin in the extracellular space, as well as additionally in the NSCs and NPCs of the neuroepithelial phenotype, which do not express nestin in the control animals, is enhanced during the traumatic process. As a result of the proliferation of such cells in the post-traumatic period, local Nes+ and Vim+ NPCs clusters are formed and become involved in the reparative response. Along with the primary traumatic lesion, which coincides with the injury zone, additional Nes+ and Vim+ secondary lesions are observed to form in the adjacent subventricular and parenchymal zones of the tegmentum. In the lateral tegmentum, the number of doublecortin-positive cells is higher compared to that in the medial tegmentum, which determines the different intensities and rates of neuronal differentiation in the sensory and motor regions of the tegmentum, respectively. In periventricular regions remote from the injury, the expression of doublecortin in single cells and their groups significantly increases compared to that in the damage zone.
Lemur Tyrosine Kinase 2 (LMTK2) Level Inversely Correlates with Phospho-Tau in Neuropathological Stages of Alzheimer’s Disease
Authors: Bencze János , Szarka Máté , Bencs Viktor , Szabó Renáta Nóra , Módis László V. , Aarsland Dag , Hortobágyi Tibor
Published: January 2020

Alzheimer’s disease (AD) is the most common neurodegenerative dementia. Mapping the pathomechanism and providing novel therapeutic options have paramount significance. Recent studies have proposed the role of LMTK2 in AD. However, its expression pattern and association with the pathognomonic neurofibrillary tangles (NFTs) in different brain regions and neuropathological stages of AD is not clear. We performed chromogenic (CHR) LMTK2 and fluorescent phospho-tau/LMTK2 double-labelling (FDL) immunohistochemistry (IHC) on 10–10 postmortem middle frontal gyrus (MFG) and anterior hippocampus (aHPC) samples with early and late neuropathological Braak tau stages of AD. MFG in early stage was our ‘endogenous control’ region as it is not affected by NFTs. Semiquantitative CHR-IHC intensity scoring revealed significantly higher (p < 0.001) LMTK2 values in this group compared to NFT-affected regions. FDL-IHC demonstrated LMTK2 predominance in the endogenous control region, while phospho-tau overburden and decreased LMTK2 immunolabelling were detected in NFT-affected groups (aHPC in early and both regions in late stage). Spearman’s correlation coefficient showed strong negative correlation between phospho-tau/LMTK2 signals within each group. According to our results, LMTK2 expression is inversely proportionate to the extent of NFT pathology, and decreased LMTK2 level is not a general feature in AD brain, rather it is characteristic of the NFT-affected regions.
GPER Activation Inhibits Cancer Cell Mechanotransduction and Basement Membrane Invasion via RhoA
Authors: Rice Alistair , Cortes Ernesto , Lachowski Dariusz , Oertle Philipp , Matellan Carlos , Thorpe Stephen D. , Ghose Ritobrata , Wang Haiyun , Lee David A. , Plodinec Marija , del Río Hernández Armando E.
Published: January 2020

The invasive properties of cancer cells are intimately linked to their mechanical phenotype, which can be regulated by intracellular biochemical signalling. Cell contractility, induced by mechanotransduction of a stiff fibrotic matrix, and the epithelial–mesenchymal transition (EMT) promote invasion. Metastasis involves cells pushing through the basement membrane into the stroma—both of which are altered in composition with cancer progression. Agonists of the G protein-coupled oestrogen receptor (GPER), such as tamoxifen, have been largely used in the clinic, and interest in GPER, which is abundantly expressed in tissues, has greatly increased despite a lack of understanding regarding the mechanisms which promote its multiple effects. Here, we show that specific activation of GPER inhibits EMT, mechanotransduction and cell contractility in cancer cells via the GTPase Ras homolog family member A (RhoA). We further show that GPER activation inhibits invasion through an in vitro basement membrane mimic, similar in structure to the pancreatic basement membrane that we reveal as an asymmetric bilayer, which differs in composition between healthy and cancer patients.
Tumor Infiltrating Neutrophils Are Enriched in Basal-Type Urothelial Bladder Cancer
Authors: Mandelli Giulio , Missale Francesco , Bresciani Debora , Benerini Gatta Luisa , Scapini Patrizia , Caveggion Elena , Roca Elisa , Bugatti Mattia , Monti Matilde , Cristinelli Luca , Belotti Sandra , Simeone Claudio , Calza Stefano , Melocchi Laura , Vermi William
Published: January 2020

Background: Urothelial bladder cancers (UBCs) are distinct in two main molecular subtypes, namely basal and luminal type. Subtypes are also diverse in term of immune contexture, providing a rationale for patient selection to immunotherapy. Methods: By digital microscopy analysis of a muscle-invasive BC (MIBC) cohort, we explored the density and clinical significance of CD66b+ tumor-associated-neutrophils (TAN) and CD3+ T cells. Bioinformatics analysis of UBC datasets and gene expression analysis of UBC cell lines were additionally performed. Results: Basal type BC contained a significantly higher density of CD66b+ TAN compared to the luminal type. This finding was validated on TCGA, GSE32894 and GSE124305 datasets by computing a neutrophil signature. Of note, basal-type MIBC display a significantly higher level of chemokines (CKs) attracting neutrophils. Moreover, pro-inflammatory stimuli significantly up-regulate CXCL1, CXCL2 and CXCL8 in 5637 and RT4 UBC cell lines and induce neutrophil chemotaxis. In term of survival, a high density of T cells and TAN was significantly associated to a better outcome, with TAN density showing a more limited statistical power and following a non-linear predicting model. Conclusions: TAN are recruited in basal type MIBC by pro-inflammatory CKs. This finding establishes a groundwork for a better understanding of the UBC immunity and its relevance.
The LisH Domain-Containing N-Terminal Fragment is Important for the Localization, Dimerization, and Stability of Katnal2 in Tetrahymena
Authors: Joachimiak Ewa , Waclawek Ewa , Niziolek Michal , Osinka Anna , Fabczak Hanna , Gaertig Jacek , Wloga Dorota
Published: January 2020

Katanin-like 2 protein (Katnal2) orthologs have a tripartite domain organization. Two highly conserved regions, an N-terminal LisH (Lis-homology) domain and a C-terminal AAA catalytic domain, are separated by a less conserved linker. The AAA domain of Katnal2 shares the highest amino acid sequence homology with the AAA domain of the canonical katanin p60. Katnal2 orthologs are present in a wide range of eukaryotes, from protists to humans. In the ciliate Tetrahymena thermophila, a Katnal2 ortholog, Kat2, co-localizes with the microtubular structures, including basal bodies and ciliary outer doublets, and this co-localization is sensitive to levels of microtubule glutamylation. The functional analysis of Kat2 domains suggests that an N-terminal fragment containing a LisH domain plays a role in the subcellular localization, dimerization, and stability of Kat2.
Regulation of Synaptic Development by Astrocyte Signaling Factors and Their Emerging Roles in Substance Abuse
Authors: Walker Christopher D. , Risher W. Christopher , Risher Mary-Louise
Published: January 2020

Astrocytes have critical functions throughout the central nervous system (CNS) and have emerged as regulators of synaptic development and function. With their highly complex morphologies, they are able to interact with thousands of synapses via peripheral astrocytic processes (PAPs), ensheathing neuronal axons and dendrites to form the tripartite synapse. In this way, astrocytes engage in crosstalk with neurons to mediate a variety of CNS processes including the regulation of extracellular matrix protein signaling, formation and maintenance of the blood-brain barrier (BBB), axon growth and guidance, homeostasis of the synaptic microenvironment, synaptogenesis, and the promotion of synaptic diversity. In this review, we discuss several key astrocyte signaling factors (thrombospondins, netrins, apolipoproteins, neuregulins, bone morphogenetic proteins, and neuroligins) in the maintenance and regulation of synapse formation. We also explore how these astrocyte signaling factors are impacted by and contribute to substance abuse, particularly alcohol and cocaine use.
Allergen-Induced C5a/C5aR1 Axis Activation in Pulmonary CD11b+ cDCs Promotes Pulmonary Tolerance through Downregulation of CD40
Authors: Antoniou Konstantina , Ender Fanny , Vollbrandt Tillman , Laumonnier Yves , Rathmann Franziska , Pasare Chandrashekhar , Singh Harinder , Köhl Jörg
Published: January 2020

Activation of the C5/C5a/C5a receptor 1 (C5aR1) axis during allergen sensitization protects from maladaptive T cell activation. To explore the underlying regulatory mechanisms, we analyzed the impact of C5aR1 activation on pulmonary CD11b+ conventional dendritic cells (cDCs) in the context of house-dust-mite (HDM) exposure. BALB/c mice were intratracheally immunized with an HDM/ovalbumin (OVA) mixture. After 24 h, we detected two CD11b+ cDC populations that could be distinguished on the basis of C5aR1 expression. C5aR1− but not C5aR1+ cDCs strongly induced T cell proliferation of OVA-reactive transgenic CD4+ T cells after re-exposure to antigen in vitro. C5aR1− cDCs expressed higher levels of MHC-II and CD40 than their C5aR1+ counterparts, which correlated directly with a higher frequency of interactions with cognate CD4+ T cells. Priming of OVA-specific T cells by C5aR1+ cDCs could be markedly increased by in vitro blockade of C5aR1 and this was associated with increased CD40 expression. Simultaneous blockade of C5aR1 and CD40L on C5aR1+ cDCs decreased T cell proliferation. Finally, pulsing with OVA-induced C5 production and its cleavage into C5a by both populations of CD11b+ cDCs. Thus, we propose a model in which allergen-induced autocrine C5a generation and subsequent C5aR1 activation in pulmonary CD11b+ cDCs promotes tolerance towards aeroallergens through downregulation of CD40.
Neuromuscular Activity Induces Paracrine Signaling and Triggers Axonal Regrowth after Injury in Microfluidic Lab-On-Chip Devices
Authors: Sala-Jarque Julia , Mesquida-Veny Francina , Badiola-Mateos Maider , Samitier Josep , Hervera Arnau , del Río José Antonio
Published: January 2020

Peripheral nerve injuries, including motor neuron axonal injury, often lead to functional impairments. Current therapies are mostly limited to surgical intervention after lesion, yet these interventions have limited success in restoring functionality. Current activity-based therapies after axonal injuries are based on trial-error approaches in which the details of the underlying cellular and molecular processes are largely unknown. Here we show the effects of the modulation of both neuronal and muscular activity with optogenetic approaches to assess the regenerative capacity of cultured motor neuron (MN) after lesion in a compartmentalized microfluidic-assisted axotomy device. With increased neuronal activity, we observed an increase in the ratio of regrowing axons after injury in our peripheral-injury model. Moreover, increasing muscular activity induces the liberation of leukemia inhibitory factor and glial cell line-derived neurotrophic factor in a paracrine fashion that in turn triggers axonal regrowth of lesioned MN in our 3D hydrogel cultures. The relevance of our findings as well as the novel approaches used in this study could be useful not only after axotomy events but also in diseases affecting MN survival.
Tendon Biomimetic Electrospun PLGA Fleeces Induce an Early Epithelial-Mesenchymal Transition and Tenogenic Differentiation on Amniotic Epithelial Stem Cells
Authors: Russo Valentina , El Khatib Mohammad , di Marcantonio Lisa , Ancora Massimo , Wyrwa Ralf , Mauro Annunziata , Walter Torsten , Weisser Jürgen , Citeroni Maria Rita , Lazzaro Francesco , Di Federico Marta , Berardinelli Paolo , Cammà Cesare , Schnabelrauch Matthias , Barboni Barbara
Published: January 2020

Background. The design of tendon biomimetic electrospun fleece with Amniotic Epithelial Stem Cells (AECs) that have shown a high tenogenic attitude may represent an alternative strategy to overcome the unsatisfactory results of conventional treatments in tendon regeneration. Methods. In this study, we evaluated AEC-engineered electrospun poly(lactide-co-glycolide) (PLGA) fleeces with highly aligned fibers (ha-PLGA) that mimic tendon extracellular matrix, their biocompatibility, and differentiation towards the tenogenic lineage. PLGA fleeces with randomly distributed fibers (rd-PLGA) were generated as control. Results. Optimal cell infiltration and biocompatibility with both PLGA fleeces were shown. However, only ha-PLGA fleeces committed AECs towards an Epithelial-Mesenchymal Transition (EMT) after 48 h culture, inducing their cellular elongation along the fibers’ axis and the upregulation of mesenchymal markers. AECs further differentiated towards tenogenic lineage as confirmed by the up-regulation of tendon-related genes and Collagen Type 1 (COL1) protein expression that, after 28 days culture, appeared extracellularly distributed along the direction of ha-PLGA fibers. Moreover, long-term co-cultures of AEC-ha-PLGA bio-hybrids with fetal tendon explants significantly accelerated of half time AEC tenogenic differentiation compared to ha-PLGA fleeces cultured only with AECs. Conclusions. The fabricated tendon biomimetic ha-PLGA fleeces induce AEC tenogenesis through an early EMT, providing a potential tendon substitute for tendon engineering research.
Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study
Authors: Vankerckhoven Ann , Wouters Roxanne , Mathivet Thomas , Ceusters Jolien , Baert Thaïs , Van Hoylandt Anaïs , Gerhardt Holger , Vergote Ignace , Coosemans An
Published: January 2020

The role of the innate immune system in ovarian cancer is gaining importance. The relevance of tumor-associated macrophages (TAM) is insufficiently understood. In this pilot project, comprising the immunofluorescent staining of 30 biopsies taken from 24 patients with ovarian cancer, we evaluated the presence of total TAM (cluster of differentiation (CD) 68 expression), M1 (major histocompatibility complex (MHC) II expression), and M2 (anti-mannose receptor C type 1 (MRC1) expression), and the blood vessel diameter. We observed a high M1/M2 ratio in low-grade ovarian cancer compared to high-grade tumors, more total TAM and M2 in metastatic biopsies, and a further increase in total TAM and M2 at interval debulking, without beneficial effects of bevacizumab. The blood vessel diameter was indicative for M2 tumor infiltration (Spearman correlation coefficient of 0.65). These data mainly reveal an immune beneficial environment in low-grade ovarian cancer in contrast to high-grade serous ovarian cancer, where immune suppression is not altered by neoadjuvant therapy.
Catalysis of an Aldol Condensation Using a Coordination Cage
Authors: Mozaceanu Cristina , Taylor Christopher G. P. , Piper Jerico R. , Argent Stephen P. , Ward Michael D.
Published: January 2020

The aldol condensation of indane-1,3-dione (ID) to give ‘bindone’ in water is catalysed by an M8L12 cubic coordination cage (Hw). The absolute rate of reaction is slow under weakly acidic conditions (pH 3–4), but in the absence of a catalyst it is undetectable. In water, the binding constant of ID in the cavity of Hw is ca. 2.4 (±1.2) × 103 M−1, giving a ∆G for the binding of −19.3 (±1.2) kJ mol−1. The crystal structure of the complex revealed the presence of two molecules of the guest ID stacked inside the cavity, giving a packing coefficient of 74% as well as another molecule hydrogen-bonded to the cage’s exterior surface. We suggest that the catalysis occurs due to the stabilisation of the enolate anion of ID by the 16+ surface of the cage, which also attracts molecules of neutral ID to the surface because of its hydrophobicity. The cage, therefore, brings together neutral ID and its enolate anion via two different interactions to catalyse the reaction, which—as the control experiments show—occurs at the exterior surface of the cage and not inside the cage cavity.
Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor
Authors: Wang Tian-Tian , Guo Kun , Hu Xue-Mei , Liang Jian , Li Xing-De , Zhang Zhi-Feng , Xie Jing
Published: January 2020

In this work, a label-free colorimetric assay was developed for the determination of urine glucose using smartphone ambient-light sensor (ALS). Using horseradish peroxidase—hydrogen peroxide—3,3′,5,5′-tetramethylbenzidine (HRP-H2O2-TMB) colored system, quantitative H2O2 was added to samples to-be-determined for deepest color. The presence of glucose oxidase in urine led to the formation of H2O2 and the reduction of TMBred. As a result of this, the color of the urine faded and the solution changed from deep blue to light blue. We measured the illuminance of the transmitted light by a smartphone ambient light sensor, and thereby color changes were used to calculate the content of urine glucose. After method validation, this colorimetric assay was practically applied for the determination of urine samples from diabetic patients. Good linearity was obtained in the range of 0.039–10.000 mg/mL (R2 = 0.998), and a limit of detection was 0.005 mg/mL. Our method was had high accuracy, sensitivity, simplicity, rapidity, and visualization, providing a new sensor to be potentially applicable for point-of-care detection of urine glucose.
Scale-Dependent Reliability of Projected Rainfalls over Bangladesh with the PRECIS Model
Authors: Mondal M. Shahjahan , Nowreen Sara , Sakib Mostofa Najmus
Published: January 2020

The regional climate model, Providing REgional Climates for Impact Studies (PRECIS), has been widely used throughout the world to generate climate change projections for impact studies and adaptations. Its recent application in South Asia also includes the projection of rainfall extremes. In spite of its wide application, a stringent validation of the model is yet to be reported. In this study, we assessed the performance of the model in simulating annual, monthly and extreme rainfalls over Bangladesh by using a number of statistical techniques, e.g., pattern (both spatial and temporal) correlation, root mean square difference (RMSD), mean absolute difference (MAD), Student’s t-test for significance, probability density functions, etc. The results indicated that the PRECIS model could capture the overall spatial pattern of mean annual and monthly rainfalls very well. However, the inter-annual variability was poorly simulated by the model. In addition, the model could not capture the rainfall extremes. A spatial aggregation of rainfall data did not improve the reliability of the model as far as variability and extremes are concerned. Therefore, further improvements of the model and/or its driving global climate model are warranted for its practical use in the generation of rainfall scenarios.
Forest Contribution to Climate Change Mitigation: Management Oriented to Carbon Capture and Storage
Authors: Nunes Leonel J.R. , Meireles Catarina I.R. , Pinto Gomes Carlos J. , Almeida Ribeiro Nuno M.C.
Published: January 2020

Today, climate change is assumed by many researchers and scholars as a certainty and is presented as the biggest challenge humanity has ever faced. It is commonly accepted that anthropogenic greenhouse gas emissions are the main cause that is accelerating the process. Therefore, it is urgent to find solutions to mitigate climate change, mainly because the intense effects have already been felt, in many cases in the form of the occurrence of extremely violent weather events. Forests are undoubtedly one of the most effective and easiest ways to provide the function of carbon sinks. However, it is essential and convenient to analyze the permanence time of this carbon in forests, because this permanence time depends directly on the forest management model used. This article aims to analyze forest management models from the perspective of carbon residence time in temperate forests, dividing the models into three types, namely carbon conservation models, carbon storage models, and carbon substitution models, according to their ability to contribute to functioning as carbon sinks, thereby contributing to the mitigation of climate change.
Independence of Future Changes of River Runoff in Europe from the Pathway to Global Warming
Authors: Mentaschi Lorenzo , Alfieri Lorenzo , Dottori Francesco , Cammalleri Carmelo , Bisselink Berny , Roo Ad De , Feyen Luc
Published: January 2020

The outcomes of the 2015 Paris Agreement triggered a number of climate impact assessments, such as for floods and droughts, to focus on future time frames corresponding to the years of reaching specific levels of global warming. Yet, the links between the timing of the warming levels and the corresponding greenhouse gas concentration pathways to reach them remain poorly understood. To address this gap, we compared projected changes of annual mean, extreme high, and extreme low river discharges in Europe at 1.5 °C and 2 °C under Representative Concentration Pathways RCP8.5 and RCP4.5 from an ensemble of regional climate model (RCM) simulations. The statistical significance of the difference between the two scenarios for both warming levels was then evaluated. The results show that in the majority of Europe (>95% of the surface area for the annual mean discharge, >98% for high and low extremes), the changes projected in the two pathways were statistically indistinguishable. These results suggest that in studies of changes at global warming levels, the projections of the two pathways can be merged into a single ensemble without major loss of information. With regard to the uncertainty of the unified ensemble, the findings show that the projected changes of annual mean, extreme high, and extreme low river discharge were statistically significant in large portions of Europe.
Influence of Mesoporous Inorganic Al–B–P Amphiprotic Surfactant Material Resistances of Wood against Brown and White-Rot Fungi (Part 1)
Authors: Yves Beaudelaire Kouomo Guelifack , Zhuang Biaorong , Aladejana John Tosin , Li Dehong , Hou Xinjun , Xie Yongqun
Published: January 2020

This study describes the application of aluminum sulfate Al2(SO4)3, boric acid H3BO3, phosphoric acid H3PO4 (Al–B–P) and amphiprotic surfactant material synthesis by the sol-gel process, which were adopted as novel precursors for wood modification. The efficacy of Al–B–P-treated wood was tested against Poria placenta and Coriolus versicolor. Untreated wood samples had higher mass losses (>40%) compared to the treated sample, which had the lowest wood mass losses (of 4%) against P. placenta and C. versicolor. To analyze the reaction mechanism of Al–B–P wood, the mechanical properties, chemical structure, crystallinity, thermal analysis, binding energy and wettability was examined by modulus of rupture (MOR), modulus of elasticity (MOE), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Thermogravimetric analysis (TG) and X-ray photoelectron spectroscopy (XPS), respectively. Scanning electron microscopy- energy-dispersive X-ray spectroscopy (SEM-EDS) confirmed the wood colonization by fungi, and was used to identify the microstructures and morphologies changes that occurred in the cells during degradation by white and brown-rot fungi. At the same time, X-ray photoelectron spectroscopy (XPS) was employed to analyze the physical and chemical properties of the samples. Therefore, the study confirmed that Al–B–P and amphiprotic surfactant could replace the traditional wood preservative products, and have the potential to extend the service life of wood, particularly in soil contact and outdoor usage.
Montmorillonite-Synergized Water-Based Intumescent Flame Retardant Coating for Plywood
Authors: Hu Xiaochun , Sun Zhao , Zhu Xiaojun , Sun Zhiqiang
Published: January 2020

In this study, montmorillonite (MMT) was used as an inorganic synergist to prepare the water-based intumescent flame retardant (IFR) ornamental coating for plywood. Results indicate that the 7 wt.% MMT modified IFR coating (No. 3) possess the best fire resistance (longer than 20 min) of the tested samples according to the fire performance, which significantly declines the specific extinction area by 44.12 m2·kg−1 compared to the coating without MMT by cone calorimeter. In addition, characterizations such as XPS, XRD, TG, SEM and FTIR were characterized to investigate the surface and bulk properties as well as the morphology of MMT synergized water-based IFR coating. It is revealed that the residual nitrogenous polyaromatic structure and 25.5% residual mass in the No. 3 coating are a result of the effect of MMT on the antioxidation properties of the char layer.
First Complete Wing of a Stem Group Sphenisciform from the Paleocene of New Zealand Sheds Light on the Evolution of the Penguin Flipper
Authors: Mayr Gerald , De Pietri Vanesa L. , Love Leigh , Mannering Al A. , Bevitt Joseph J. , Scofield R. Paul
Published: January 2020

We describe a partial skeleton of a stem group penguin from the Waipara Greensand in New Zealand, which is tentatively assigned to Muriwaimanu tuatahi. The fossil includes the first complete wing of a Paleocene penguin and informs on previously unknown features of the mandible and tibiotarsus of small-sized Sphenisciformes from the Waipara Greensand. The wing is distinguished by important features from that of all geologically younger Sphenisciformes and documents an early stage in the evolution of wing-propelled diving in penguins. In particular, the wing of the new fossil exhibits a well-developed alular phalanx and the distal phalanges are not flattened. Because the wing phalanges resemble those of volant birds, we consider it likely that the wing feathers remained differentiated into functional categories and were not short and scale-like as they are in extant penguins. Even though the flippers of geologically younger penguins may favor survival in extremely cold climates, they are likely to have been shaped by hydrodynamic demands. Possible selective drivers include a diminished importance of the hindlimbs in subaquatic propulsion, new foraging strategies (the caudal end of the mandible of the new fossil distinctly differs from that of extant penguins), or increased predation by marine mammals.
Elevational Patterns of Generic Diversity in the Tenebrionid Beetles (Coleoptera Tenebrionidae) of Latium (Central Italy)
Authors: Fattorini Simone , Mantoni Cristina , Di Biase Letizia , Strona Giovanni , Pace Loretta , Biondi Maurizio
Published: January 2020

The concept of generic diversity expresses the ‘diversification’ of species into genera in a community. Since niche overlap is assumed to be higher in congeneric species, competition should increase generic diversity. On the other hand, generic diversity might be lower in highly selective environments, where only species with similar adaptations can survive. We used the distribution of tenebrionid beetles in Central Italy to investigate how generic diversity varies with elevation from sea level to 2400 m altitude. Generic diversity of geophilous tenebrionids decreased sharply with elevation, whereas the generic diversity of xylophilous tenebrionids showed similarly high values across the gradient. These results suggest that geophilous species are more sensitive to variation in environmental factors, and that the advantages of close relationships (similar adaptations to harsh conditions) are greater than the possible drawbacks (competition). This is consistent with the fact that geophilous tenebrionids are mostly generalist detritivores, and hence weakly affected by competition for resources. By contrast, xylophilous species are more protected from harsh/selective conditions, but more limited by competition for microhabitats and food. Our results support the environmental filtering hypothesis for the species composition of tenebrionid beetles along an elevational gradient.
Genetic Structure and Connectivity of the Red Mangrove at Different Geographic Scales through a Complex Transverse Hydrological System from Freshwater to Marine Ecosystems
Authors: Chablé Iuit Landy R. , Machkour-M’Rabet Salima , Espinoza-Ávalos Julio , Hernández-Arana Héctor A. , López-Adame Haydée , Hénaut Yann
Published: January 2020

Mangrove forests are ecologically and economically valuable resources composed of trees morphologically and physiologically adapted to thrive across a range of habitats. Although, mangrove trees have high dispersion capacity, complexity of hydrological systems may lead to a fine-scale genetic structure (FSGS). The Transverse Coastal Corridor (TCC) is an interesting case of hydrological systems from fresh to marine waters where mangrove forests dominate. We evaluated genetic diversity and structure of Rhizophora mangle across a range of hydrological conditions within the TCC using inter-simple sequence repeat molecular markers. Sampling included four hydrological systems, two localities inside each system, and fringe and dwarf trees. Genetic differentiation was evaluated at local (<100 km) and fine (<10 km) scales through a set of analyses, and genetic diversity was evaluated at all scale levels and between fringe and dwarf physiognomic types. Rhizophora mangle exhibited a high genetic structure at both scales with high genetic diversity. The genetic structure observed among hydrological systems likely reflects the historical dispersion of mangroves, whereas the FSGS reflect contemporary processes such as seed dispersal restriction, habitat fragmentation, and local water flow regimes. A higher genetic diversity for dwarf than for fringe trees and differentiation between both physiognomic types at a fine-scale were observed and discussed.
Camptocormia as a Novel Phenotype in a Heterozygous POLG2 Mutation
Authors: Lehmann Urban Diana , Motlagh Scholle Leila , Alt Kerstin , Ludolph Albert C. , Rosenbohm Angela
Published: January 2020

Mitochondrial dysfunction is known to play a key role in the pathophysiological pathway of neurodegenerative disorders. Nuclear-encoded proteins are involved in mtDNA replication, including DNA polymerase gamma, which is the only known replicative mtDNA polymerase, encoded by nuclear genes Polymerase gamma 1 (POLG) and Polymerase gamma 2 (POLG2). POLG mutations are well-known as a frequent cause of mitochondrial myopathies of nuclear origin. However, only rare descriptions of POLG2 mutations leading to mitochondriopathies exist. Here we describe a 68-year-old woman presenting with a 20-year history of camptocormia, mild proximal weakness, and moderate CK increase. Muscle histology showed COX-negative fibres. Genetic analysis by next generation sequencing revealed an already reported heterozygous c.1192-8_1207dup24 mutation in the POLG2 gene. This is the first report on a POLG2 mutation leading to camptocormia as the main clinical phenotype, extending the phenotypic spectrum of POLG2 associated diseases. This underlines the broad phenotypic spectrum found in mitochondrial diseases, especially in mitochondrial disorders of nuclear origin.
Circulating Anti-Sorting Nexins 16 Antibodies as an Emerging Biomarker of Coronary Artery Disease in Patients with Obstructive Sleep Apnea
Authors: Katsumata Yusuke , Terada Jiro , Matsumura Takuma , Koshikawa Ken , Sakao Seiichiro , Tomiyoshi Go , Shinmen Natsuko , Nakamura Rika , Kuroda Hideyuki , Nagashima Kengo , Kobayashi Yoshio , Kobayashi Eiichi , Iwadate Yasuo , Zhang Xiao-Meng , Hiwasa Takaki , Tatsumi Koichiro
Published: January 2020

Biomarkers are not available for monitoring the onset and progression of coronary artery disease (CAD) in patients with obstructive sleep apnea (OSA), a major risk factor for arteriosclerotic cardiovascular diseases. This study aimed to test for correlation between circulating anti-Sorting Nexins 16 antibody (SNX16-Ab) levels, CAD history and clinical parameters of patients with OSA. Sixty-four healthy donors, 82 adults with OSA, and 96 with acute coronary syndrome (ACS) were studied. Serum samples were collected at diagnostic polysomnography in the OSA group or at the disease onset in the ACS group. Serum SNX16-Ab levels were measured by amplified luminescence proximity homogeneous assay (AlphaLISA), and correlation between SNX16-Ab levels and clinical parameters was analyzed. SNX16-Ab levels and apnea-hypopnea index (AHI) were weakly correlated. Additionally, logistic regression analyses of OSA group identified that elevated SNX16-Ab level associated with the history of CAD. Circulating SNX16-Ab could increase during CAD pathogenesis in patients with OSA. Further prospective studies are required to prove the predictive potential of SNX16-Ab level in CAD onset of patients with OSA.
High-Payload Data-Hiding Method for AMBTC Decompressed Images
Authors: Yeh Jung-Yao , Chen Chih-Cheng , Liu Po-Liang , Huang Ying-Hsuan
Published: January 2020

Data hiding is the art of embedding data into a cover image without any perceptual distortion of the cover image. Moreover, data hiding is a very crucial research topic in information security because it can be used for various applications. In this study, we proposed a high-capacity data-hiding scheme for absolute moment block truncation coding (AMBTC) decompressed images. We statistically analyzed the composition of the secret data string and developed a unique encoding and decoding dictionary search for adjusting pixel values. The dictionary was used in the embedding and extraction stages. The dictionary provides high data-hiding capacity because the secret data was compressed using dictionary-based coding. The experimental results of this study reveal that the proposed scheme is better than the existing schemes, with respect to the data-hiding capacity and visual quality.
Analytic Expression of Quantum Discords in Werner States under LQCC
Authors: Xie Chuanmei , Zhang Zhanjun , Chen Jianlan , Yin Xiaofeng
Published: January 2020

In this paper, quantum discords in a special kind of states, i.e., Werner states by local quantum operations and classical communication (LQCC) protocols (WLQCC states), are studied. Nineteen parameters to quantify the quantum discords are reduced to four parameters in terms of properties of Werner states and quantum discord. In the case of orthogonal projective measures, analytic expression of quantum discords in WLQCC states is analytically worked out. Some properties of the quantum discord in the WLQCC states are obtained, especially the variation relations between the quantum discords and the parameters characterizing the WLQCC states. By virtue of numerical computations, quantum discords in a Werner state before and after LQCC protocols are compared. It is found that quantum discord in any WLQCC state cannot exceed that in the original Werner state.
Comprehensive Evaluation on Soil Properties and Artemisia ordosica Growth under Combined Application of Fly Ash and Polyacrylamide in North China
Authors: Niu Jiping , Su Xiaoling , Tang Zejun , Lu Kaiwen , Zhang Gengxi , Wang Fengxin , Wang Jie
Published: January 2020

A field experiment was conducted to investigate the combined application effects of fly ash (FA) (0, 5%, 10%, and 15% (w/w) soil) and polyacrylamide (PAM) (0, 0.006% and 0.012% (w/w) soil) on the edge of Hobq Desert in Inner Mongolia, China from May 2016 to October 2018. Seven different ratios of FA and PAM were selected as evaluation objects, a total of 14 soil property indices and 9 Artemisia ordosica growth indices were selected as evaluation indicators, and the entropy weight method was employed to evaluate the soil physicochemical properties and vegetation growth performances under FA and PAM amendments. The results showed that the F15P1 (15% FA + 0.006% PAM) and F5P1 (5% FA + 0.006% PAM) were the effective treatments for soil improvement and Artemisia ordosica growth respectively. Considering the soil properties and Artemisia ordosica growth in 2016–2018 synthetically, the highest score was observed in the F5P1, followed by the F5P2 (5% FA + 0.012% PAM) and F10P1 (10% FA + 0.006% PAM) treatments. The optimal amounts for FA and PAM should be recommended as 5% and 0.006%, respectively.
Dynamics and Complexity of Computrons
Authors: Erkurt Murat
Published: January 2020

We investigate chaoticity and complexity of a binary general network automata of finite size with external input which we call a computron. As a generalization of cellular automata, computrons can have non-uniform cell rules, non-regular cell connectivity and an external input. We show that any finite-state machine can be represented as a computron and develop two novel set-theoretic concepts: (i) diversity space as a metric space that captures similarity of configurations on a given graph and (ii) basin complexity as a measure of complexity of partitions of the diversity space. We use these concepts to quantify chaoticity of computrons’ dynamics and the complexity of their basins of attraction. The theory is then extended into probabilistic machines where we define fuzzy basin partitioning of recurrent classes and introduce the concept of ergodic decomposition. A case study on 1D cyclic computron is provided with both deterministic and probabilistic versions.
Gaussian Process Regression for Data Fulfilling Linear Differential Equations with Localized Sources
Authors: Albert Christopher G. , Rath Katharina
Published: January 2020

Specialized Gaussian process regression is presented for data that are known to fulfill a given linear differential equation with vanishing or localized sources. The method allows estimation of system parameters as well as strength and location of point sources. It is applicable to a wide range of data from measurement and simulation. The underlying principle is the well-known invariance of the Gaussian probability distribution under linear operators, in particular differentiation. In contrast to approaches with a generic covariance function/kernel, we restrict the Gaussian process to generate only solutions of the homogeneous part of the differential equation. This requires specialized kernels with a direct correspondence of certain kernel hyperparameters to parameters in the underlying equation and leads to more reliable regression results with less training data. Inhomogeneous contributions from linear superposition of point sources are treated via a linear model over fundamental solutions. Maximum likelihood estimates for hyperparameters and source positions are obtained by nonlinear optimization. For differential equations representing laws of physics the present approach generates only physically possible solutions, and estimated hyperparameters represent physical properties. After a general derivation, modeling of source-free data and parameter estimation is demonstrated for Laplace’s equation and the heat/diffusion equation. Finally, the Helmholtz equation with point sources is treated, representing scalar wave data such as acoustic pressure in the frequency domain.
A Thin Layer of Activated Carbon Deposited on Polyurethane Cube Leads to New Conductive Bioanode for (Plant) Microbial Fuel Cell
Authors: Sudirjo Emilius , Diaz Paola Y. Constantino , Cociancich Matteo , Lisman Rens , Snik Christian , Buisman Cees J. N. , Strik David P. B. T. B.
Published: January 2020

Large-scale implementation of (plant) microbial fuel cells is greatly limited by high electrode costs. In this work, the potential of exploiting electrochemically active self-assembled biofilms in fabricating three-dimensional bioelectrodes for (plant) microbial fuel cells with minimum use of electrode materials was studied. Three-dimensional robust bioanodes were successfully developed with inexpensive polyurethane foams (PU) and activated carbon (AC). The PU/AC electrode bases were fabricated via a water-based sorption of AC particles on the surface of the PU cubes. The electrical current was enhanced by growth of bacteria on the PU/AC bioanode while sole current collectors produced minor current. Growth and electrochemical activity of the biofilm were shown with SEM imaging and DNA sequencing of the microbial community. The electric conductivity of the PU/AC electrode enhanced over time during bioanode development. The maximum current and power density of an acetate fed MFC reached 3 mA·m−2 projected surface area of anode compartment and 22 mW·m−3 anode compartment. The field test of the Plant-MFC reached a maximum performance of 0.9 mW·m−2 plant growth area (PGA) at a current density of 5.6 mA·m−2 PGA. A paddy field test showed that the PU/AC electrode was suitable as an anode material in combination with a graphite felt cathode. Finally, this study offers insights on the role of electrochemically active biofilms as natural enhancers of the conductivity of electrodes and as transformers of inert low-cost electrode materials into living electron acceptors.
Utilization of CO2 as Cushion Gas for Depleted Gas Reservoir Transformed Gas Storage Reservoir
Authors: Cao Cheng , Liao Jianxing , Hou Zhengmeng , Xu Hongcheng , Mehmood Faisal , Wu Xuning
Published: January 2020

Underground gas storage reservoirs (UGSRs) are used to keep the natural gas supply smooth. Native natural gas is commonly used as cushion gas to maintain the reservoir pressure and cannot be extracted in the depleted gas reservoir transformed UGSR, which leads to wasting huge amounts of this natural energy resource. CO2 is an alternative gas to avoid this particular issue. However, the mixing of CO2 and CH4 in the UGSR challenges the application of CO2 as cushion gas. In this work, the Donghae gas reservoir is used to investigate the suitability of using CO2 as cushion gas in depleted gas reservoir transformed UGSR. The impact of the geological and engineering parameters, including the CO2 fraction for cushion gas, reservoir temperature, reservoir permeability, residual water and production rate, on the reservoir pressure, gas mixing behavior, and CO2 production are analyzed detailly based on the 15 years cyclic gas injection and production. The results showed that the maximum accepted CO2 concentration for cushion gas is 9% under the condition of production and injection for 120 d and 180 d in a production cycle at a rate of 4.05 kg/s and 2.7 kg/s, respectively. The typical curve of the mixing zone thickness can be divided into four stages, which include the increasing stage, the smooth stage, the suddenly increasing stage, and the periodic change stage. In the periodic change stage, the mixed zone increases with the increasing of CO2 fraction, temperature, production rate, and the decreasing of permeability and water saturation. The CO2 fraction in cushion gas, reservoir permeability, and production rate have a significant effect on the breakthrough of CO2 in the production well, while the effect of water saturation and temperature is limited.
Characterizing Meteorological Forecast Impact on Microgrid Optimization Performance and Design
Authors: Jane Robert , Parker Gordon , Vaucher Gail , Berman Morris
Published: January 2020

A microgrid consists of electrical generation sources, energy storage assets, loads, and the ability to function independently, or connect and share power with other electrical grids. Thefocus of this work is on the behavior of a microgrid, with both diesel generator and photovoltaic resources, whose heating or cooling loads are influenced by local meteorological conditions. Themicrogrid's fuel consumption and energy storage requirement were then examined as a function of the atmospheric conditions used by its energy management strategy (EMS). A fuel-optimal EMS, able to exploit meteorological forecasts, was developed and evaluated using a hybrid microgrid simulation. Weather forecast update periods ranged from 15 min to 24 h. Four representative meteorological sky classifications (clear, partly cloudy, overcast, or monsoon) were considered. Forall four sky classifications, fuel consumption and energy storage requirements increased linearly with the increasing weather forecast interval. Larger forecast intervals lead to degraded weather forecasts, requiring more frequent charging/discharging of the energy storage, increasing both the fuel consumption and energy storage design requirements. The significant contributions of this work include the optimal EMS and an approach for quantifying the meteorological forecast effects on fuel consumption and energy storage requirements on microgrid performance. The findings of this study indicate that the forecast interval used by the EMS affected both fuel consumption and energy storage requirements, and that the sensitivity of these effects depended on the 24-hour sky conditions.
Enhanced Performance Modified Discontinuous PWM Technique for Three-Phase Z-Source Inverter
Authors: Hossameldin Ahmed A. , Abdelsalam Ahmed K. , Ibrahim Ahmed A. , Williams Barry W.
Published: January 2020

Various industrial applications require a voltage conversion stage from DC to AC. Among them, commercial renewable energy systems (RES) need a voltage buck and/or boost stage for islanded/grid connected operation. Despite the excellent performance offered by conventional two-stage converter systems (dc–dc followed by dc–ac stages), the need for a single-stage conversion stage is attracting more interest for cost and size reduction reasons. Although voltage source inverters (VSIs) are voltage buck-only converters, single stage current source inverters (CSIs) can offer voltage boost features, although at the penalty of using a large DC-link inductor. Boost inverters are a good candidate with the demerit of complicated control strategies. The impedance source (Z-source) inverter is a high-performance competitor as it offers voltage buck/boost in addition to a reduced passive component size. Several pulse width modulation (PWM) techniques have been presented in the literature for three-phase Z-source inverters. Various common drawbacks are annotated, especially the non-linear behavior at low modulation indices and the famous trade-off between the operating range and the converter switches’ voltage stress. In this paper, a modified discontinuous PWM technique is proposed for a three-phase z-source inverter offering: (i) smooth voltage gain variation, (ii) a wide operating range, (iii) reduced voltage stress, and (iv) improved total harmonic distortion (THD). Simulation, in addition to experimental results at various operating conditions, validated the proposed PWM technique’s superior performance compared to the conventional PWM techniques.