bluuebunny/crossref_metadata_2025 huggingface dataset:
A functional reference map of the RNF8 interactome in cancer
Authors: Liu Chuanyang , Kuang Jingyu , Wang Yuxuan , Duan Ting , Min Lu , Lu Chenyu , Zhang Tianyi , Chen Ruifen , Wu Ying , Zhu Lingyun
Published: July 2022

Background

RNF8 is an E3 ligase identified as a critical DNA damage-responsive protein. Recently, multiple reports have shown that RNF8 could be used as an important therapeutic target for cancer chemo/radiotherapy. However, the understanding of RNF8 remains limited due to the lack of its interactome reference map and comprehensive analysis of RNF8 in diverse cancers, which underscores the need to map the interactome of RNF8 via high-throughput methods.

Results

A two-way identification method based on LC–MS was designed for the identification of the RNF8 interactome with high-specificity. By in silico analysis and in vitro validation, we identified a new reference map of the RNF8 interactome network containing many new targets, such as YBX1, DNMT1, and HDCA1, new biological functions and the gene-disease associations of RNF8. Our results revealed a close relationship between RNF8 and neurodegenerative diseases or tumor-infiltrating immune cells using bulk RNA-seq and scRNA-seq datasets. As a proof of concept of our interactome map, we validated the direct binding between RNF8 and YBX1 and showed that RNF8 catalyzed the ubiquitination of YBX1. These results demonstrated that RNF8 might be a crucial regulator of YBX1.

Conclusions

Our work provides a unique framework for researchers and clinicians who seek to better explore or understand RNF8-regulated biological functions in cancers. This study will hopefully facilitate the rational design and further development of anti-RNF8 therapy in cancers.

Graphical abstract
Treatment of dogs with fluralaner reduced pyrethroid-resistant Triatoma infestans abundance, Trypanosoma cruzi infection and human-triatomine contact in the Argentine Chaco
Authors: Gürtler Ricardo Esteban , Laiño Mariano Alberto , Alvedro Alejandra , Enriquez Gustavo Fabián , Macchiaverna Natalia Paula , Gaspe María Sol , Cardinal Marta Victoria
Published: July 2022

Background

Triatomine elimination efforts and the interruption of domestic transmission of Trypanosoma cruzi are hampered by pyrethroid resistance. Fluralaner, a long-lasting ectoparasiticide administered to dogs, substantially reduced site infestation and abundance of pyrethroid-resistant Triatoma infestans Klug (Heteroptera: Reduviidae) in an ongoing 10-month trial in Castelli (Chaco Province, Argentina). We assessed the effects of fluralaner on vector infection with T. cruzi and blood meal sources stratified by ecotope and quantified its medium-term effects on site infestation and triatomine abundance.

Methods

We conducted a placebo-controlled, before-and-after efficacy trial of fluralaner in 28 infested sites over a 22-month period. All dogs received either an oral dose of fluralaner (treated group) or placebo (control group) at 0 month post-treatment [MPT]. Placebo-treated dogs were rescue-treated with fluralaner at 1 MPT, as were all eligible dogs at 7 MPT. Site-level infestation and abundance were periodically assessed by timed manual searches with a dislodging aerosol. Vector infection was mainly determined by kDNA-PCR and blood meal sources were determined by enzyme-linked immunosorbent assay.

Results

In fluralaner-treated households, site infestation dropped from 100% at 0 MPT to 18–19% over the period 6–22 MPT while mean abundance plummeted from 5.5 to 0.6 triatomines per unit effort. In control households, infestation dropped similarly post-treatment. The overall prevalence of T. cruzi infection steadily decreased from 13.8% at 0–1 MPT (baseline) to 6.4% and subsequently 2.3% thereafter, while in domiciles, kitchens and storerooms it dropped from 17.4% to 4.7% and subsequently 3.3% thereafter. Most infected triatomines occurred in domiciles and had fed on humans. Infected-bug abundance plummeted after fluralaner treatment and remained marginal or nil thereafter. The human blood index of triatomines collected in domiciles, kitchens and storerooms highly significantly fell from 42.9% at baseline to 5.3–9.1% over the period 6–10 MPT, increasing to 36.8% at 22 MPT. Dog blood meals occurred before fluralaner administration only. The cat blood index increased from 9.9% at baseline to 57.9–72.7% over the period 6–10 MPT and dropped to 5.3% at 22 MPT, whereas chicken blood meals rose from 39.6% to 63.2–88.6%.

Conclusion

Fluralaner severely impacted infestation- and transmission-related indices over nearly 2 years, causing evident effects at 1 MPT, and deserves larger efficacy trials.

Graphical Abstract
TSG-6 released from adipose stem cells-derived small extracellular vesicle protects against spinal cord ischemia reperfusion injury by inhibiting endoplasmic reticulum stress
Authors: Lu Xiao , Lv Chengtang , Zhao Yuechao , Wang Yufei , Li Yao , Ji Chengyue , Wang Zhuanghui , Ye Wu , Yu Shunzhi , Bai Jianling , Cai Weihua
Published: July 2022

Background

Spinal cord ischemia reperfusion injury (SCIRI) is a complication of aortic aneurysm repair or spinal cord surgery that is associated with permanent neurological deficits. Mesenchymal stem cell (MSC)-derived small extracellular vesicles (sEVs) have been shown to be potential therapeutic options for improving motor functions after SCIRI. Due to their easy access and multi-directional differentiation potential, adipose‐derived stem cells (ADSCs) are preferable for this application. However, the effects of ADSC-derived sEVs (ADSC-sEVs) on SCIRI have not been reported.

Results

We found that ADSC-sEVs inhibited SCIRI-induced neuronal apoptosis, degradation of tight junction proteins and suppressed endoplasmic reticulum (ER) stress. However, in the presence of the ER stress inducer, tunicamycin, its anti-apoptotic and blood–spinal cord barrier (BSCB) protective effects were significantly reversed. We found that ADSC-sEVs contain tumor necrosis factor (TNF)-stimulated gene-6 (TSG-6) whose overexpression inhibited ER stress in vivo by modulating the PI3K/AKT pathway.

Conclusions

ADSC-sEVs inhibit neuronal apoptosis and BSCB disruption in SCIRI by transmitting TSG-6, which suppresses ER stress by modulating the PI3K/AKT pathway.
Lipid metabolism and storage in neuroglia: role in brain development and neurodegenerative diseases
Authors: Yang Danying , Wang Xifeng , Zhang Lieliang , Fang Yang , Zheng Qingcui , Liu Xing , Yu Wen , Chen Shoulin , Ying Jun , Hua Fuzhou
Published: July 2022

The importance of neuroglia in maintaining normal brain function under physiological and pathological conditions has been supported by growing evidence in recent years. The most important issues regarding glial metabolism and function include the cooperation between glial populations and neurons, morphological and functional changes in pathological states, and the role in the onset and progression of neurodegenerative diseases. Although lipid accumulation and further lipid droplet production in neurodegenerative disease brain models have been observed for a long time, the dynamic development of brain lipid droplet research in recent years suggests its role in the development and progression of neurodegenerative diseases was previously underestimated. First recognized as organelles of lipid storage, lipid droplets (LDs) have emerged as an important organelle in metabolic diseases, inflammation, and host defense. Dynamic changes in lipid metabolism within neurons and glial cells resulting in lipid accumulation and lipid droplet formation are present in brain models of various neurodegenerative diseases, yet their role in the brain remains largely unexplored. This paper first reviews the metabolism and accumulation of several major lipids in the brain and discusses the regulation of lipid accumulation in different types of brain cells. We explore the potential role of intracellular lipid accumulation in the pathogenesis of neurodegeneration, starting from lipid metabolism and LDs biogenesis in glial cells, and discuss several pathological factors that promote lipid droplet formation, mainly focusing on oxidative stress, energy metabolism and glial cell-neuron coupling, which are closely related to the etiology and progression of neurodegenerative diseases. Finally, the directions and challenges of intracellular lipid metabolism in glial cells in neurodegeneration are discussed.
Transcriptomic analysis reveals the key role of histone deacetylation via mediating different phytohormone signalings in fiber initiation of cotton
Authors: Wei Zhenzhen , Li Yonghui , Ali Faiza , Wang Ye , Liu Jisheng , Yang Zuoren , Wang Zhi , Xing Yadi , Li Fuguang
Published: July 2022

Background

Histone deacetylation is one of the most important epigenetic modifications and plays diverse roles in plant development. However, the detailed functions and mechanisms of histone deacetylation in fiber development of cotton are still unclear. HDAC inhibitors (HDACi) have been commonly used to study the molecular mechanism underlying histone deacetylation or to facilitate disease therapy in humans through hindering the histone deacetylase catalytic activity. Trichostatin A (TSA)—the most widely used HDACi has been extensively employed to determine the role of histone deacetylation on different developmental stages of plants.

Results

Through in vitro culture of ovules, we observed that exogenous application of TSA was able to inhibit the fiber initiation development. Subsequently, we performed a transcriptomic analysis to reveal the underlying mechanisms. The data showed that TSA treatment resulted in 4209 differentially expressed genes, which were mostly enriched in plant hormone signal transduction, phenylpropanoid biosynthesis, photosynthesis, and carbon metabolism pathways. The phytohormone signal transduction pathways harbor the most differentially expressed genes. Deeper studies showed that some genes promoting auxin, Gibberellic Acid (GA) signaling were down-regulated, while some genes facilitating Abscisic Acid (ABA) and inhibiting Jasmonic Acid (JA) signaling were up-regulated after the TSA treatments. Further analysis of plant hormone contents proved that TSA significantly promoted the accumulation of ABA, JA and GA3.

Conclusions

Collectively, histone deacetylation can regulate some key genes involved in different phytohormone pathways, and consequently promoting the auxin, GA, and JA signaling, whereas repressing the ABA synthesis and signaling to improve the fiber cell initiation. Moreover, the genes associated with energy metabolism, phenylpropanoid, and glutathione metabolism were also regulated by histone deacetylation. The above results provided novel clues to illuminate the underlying mechanisms of epigenetic modifications as well as related different phytohormones in fiber cell differentiation, which is also very valuable for the molecular breeding of higher quality cotton.
Human skin dermis-derived fibroblasts are a kind of functional mesenchymal stromal cells: judgements from surface markers, biological characteristics, to therapeutic efficacy
Authors: Tai Chenxu , Xie Zhengyao , Li Yu , Feng Yirui , Xie Yuanyuan , Yang Hui , Wang Liudi , Wang Bin
Published: July 2022

Background

Human mesenchymal stromal cells (MSCs) have been widely advocated to clinical use. Human skin dermis-derived fibroblasts shared similar cellular morphology and biological characteristics to MSCs, while it still keeps elusive whether fibroblasts are functionally equivalent to MSCs for therapeutic use.

Methods

We isolated various fibroblasts derived from human foreskins (HFFs) and human double-fold eyelids (HDF) and MSCs derived from human umbilical cords (UC-MSCs), and then comprehensively investigated their similarities and differences in morphology, surface markers, immunoregulation, multilineage differentiation, transcriptome sequencing, and metabolomics, and therapeutic efficacies in treating 2,4,6-Trinitrobenzenesulfonic acid (TNBS) induced colitis and carbontetrachloride (CCL4) induced liver fibrosis.

Results

Fibroblasts and UC-MSCs shared similar surface markers, strong multilineage differentiation capacity, ability of inhibiting Th1/Th17 differentiation and promoting Treg differentiation in vitro, great similarities in mRNA expression profile and metabolites, and nearly equivalent therapeutic efficacy on TNBS-induced colitis and CCL4-induced hepatic fibrosis.

Conclusion

Human skin dermis-derived fibroblasts were a kind of functional MSCs with functionally equivalent therapeutic efficacy in treating specific complications, indicating fibroblasts potentially had the same lineage hierarchy of origin as MSCs and had a remarkable potential as an alternative to MSCs in the treatment of a variety of diseases.
Infant formulas with synthetic oligosaccharides and respective marketing practices: Position Statement of the German Society for Child and Adolescent Medicine e.V. (DGKJ), Commission for Nutrition
Authors: Bührer Christoph , Ensenauer Regina , Jochum Frank , Kalhoff Hermann , Koletzko Berthold , Lawrenz Burkhard , Mihatsch Walter , Posovszky Carsten , Rudloff Silvia
Published: July 2022

Human milk contains more than 150 different oligosaccharides, which together are among to the quantitatively predominant solid components of breast milk. The oligosaccharide content and composition of human milk show large inter-individual differences. Oligosaccharide content is mostly influenced by genetic variants of the mother’s secretor status. Oligosaccharides in human milk are utilized by infants’ intestinal bacteria, affecting bacterial composition and metabolic activity. Maternal secretor status, and respective differing fucosylated oligosaccharide content, has been associated both with reduced and increased risk of infection in different populations of breastfed infants, possibly due to environmental conditions and the infant’s genotype. There are no safety concerns regarding the addition of previously approved oligosaccharides to infant formula; however, no firm conclusions can be drawn about clinically relevant benefits either. Therefore, infant formulas with synthetic oligosaccharide additives are currently not preferentially recommended over infant formulas without such additives. We consider the use of terms such as “human milk oligosaccharides” and corresponding abbreviations such as “HMO” in any advertising of infant formula to be an inappropriate idealization of infant formula. Manufacturers should stop this practice, and such marketing practices should be prevented by responsible supervisory authorities. Pediatricians should inform families that infant formulas supplemented with synthetic oligosaccharides do not resemble the complex oligosaccharide composition of human milk.
Assessment of the genetic variations of sago palm Metroxylon sagu in three regions of Sarawak, Malaysia using amplified fragment length polymorphism (AFLP) marker
Authors: Nisar Mehvish , Hussain Hasnain
Published: July 2022

Background

Sago palm (Metroxylon sagu) is an important staple crop in the rural area of Sarawak, Malaysia. The palms grow well in the rough, swampy peat, from low flooded areas to uplands and acidic to neutral soils. Among the starch-producing crops, this palm is the most productive and promising, where it can store a significant amount of carbohydrate in the trunk. Due to the lack of molecular study of sago palm, the work described here aimed to develop molecular markers for identifying M. sagu and accessing the genetic variations of sago palm in different locations of Sarawak.

Results

Amplified fragment length polymorphism was employed to determine the genetic variations of sago palm between fifteen populations collected from three different locations: Mukah, Pusa and Samarahan. Based on 35,016 polymorphic fragments generated from 32 primer combinations, polymorphic information content (PIC), marker index (MI) and resolving power (RP) averaged 0.269, 0.005157 and 51.52039 per primer combination, respectively, were obtained, signifying the effectiveness and accuracy of the AFLP marker. Mukah showed higher diversity (I = 0.428, H = 0.232), while Pusa showed the lowest (I = 0.367, H = 0.240). The average value of the Shannon information index was 0.404. AMOVA showed 99% of the variation was found within the population. The Mantel test was performed with 9999 permutations. A significantly positive correlation (r = 0.220, p = 0.010) was observed between the genetic divergence of the population (Nei genetic distance) with the geographical distance.

Conclusions

Our study considered AFLP is as an effective and reliable method to assess the genetic variations and the identification of M. sagu.

Graphical Abstract
Echocardiographic description and outcomes in a heterogeneous cohort of patients undergoing mitral valve surgery with and without mitral annular disjunction: a health service evaluation
Authors: Bennett Sadie , Tafuro Jacopo , Brumpton Marcus , Bardolia Caragh , Heatlie Grant , Duckett Simon , Ridley Paul , Nanjaiah Prakash , Kwok Chun Shing
Published: July 2022

Background

Mitral annular disjunction (MAD) is a structural abnormality characterized by the distinct separation of the mitral valve annulus/left atrium wall and myocardium. Little is known about the significance of MAD in patients requiring mitral valve surgery. This evaluation evaluates the echocardiographic characteristics and patient outcomes for patients with and without MAD who require mitral valve surgery.

Methods

All patients who underwent mitral valve surgery and who had a pre-surgical transthoracic echocardiogram between 2013 and 2020 were included. Patient demographics and clinical outcomes were collected on review of patient electronic records.

Results

A total of 185 patients were included in the analysis of which 32.4% had MAD (average MAD length 8.4 mm). MAD was seen most commonly in patients with mitral valve prolapse and myxomatous mitral valves disease (90% and 60% respectively). In the patients with MAD prior to mitral valve surgery, only 3.9% had MAD post mitral valve surgery. There were no significant difference in the severity of post-operative mitral regurgitation, arrhythmic events or major adverse cardiovascular events in patients with and without MAD.

Conclusions

MAD is common in patients who undergo mitral valve surgery. Current surgical techniques are able to correct the MAD abnormality in the vast majority of patients. MAD is not associated with an increased risk of adverse clinical outcomes post mitral valve surgery.
Dual Wasserstein generative adversarial network condition: A generative adversarial network-based acoustic impedance inversion method
Authors: Wang Zixu , Wang Shoudong , Zhou Chen , Cheng Wanli
Published: July 2022

Deep learning neural networks offer some advantages over conventional methods in acoustic impedance inversion. Because labeled data may be difficult to obtain in realistic field data settings, it can be difficult to obtain high-accuracy inversion results. Some generative adversarial network (GAN)-based acoustic impedance inversion methods have been proposed to solve this problem. However, due to the existence of lateral discontinuity in inversion results of these GAN-based methods, inversion accuracy of these proposals is still not fully satisfactory. Therefore, to tackle the shortcomings of GAN, we develop an acoustic impedance inversion method based on dual Wasserstein GAN condition (dual-WGAN-c). Dual-WGAN-c can perform seismic inversion and forward calculation simultaneously and take the low-frequency information of acoustic impedance as conditional input to obtain accurate acoustic impedance inversion results in the case of insufficient labeled data. Through a data simulation experiment, compared with GAN-based seismic acoustic impedance inversion methods, dual-WGAN-c can obtain more accurate inversion results, with high robustness when the seismic data are noisy. Dual-WGAN-c also can obtain accurate results in acoustic impedance inversion of the recorded seismic data when one has insufficient labeled well-log data.
Idiopathic Spinal Subarachnoid and Subdural Hemorrhage in a Patient With Eosinophilic Granulomatosis and Polyangiitis
Authors: Smith William J. , Kanter John H. , Jaleel Naser , Aronson Joshua P.
Published: July 2022

BACKGROUND AND IMPORTANCE:

Development of concomitant spontaneous subdural hematoma (SSDH) and spontaneous subarachnoid hemorrhage (SSAH) in the spine without inciting trauma, iatrogenic event, or underlying cerebrovascular malformation is rare. It is rarer still for these to present in a patient with eosinophilic granulomatosus with polyangiitis.

CLINICAL PRESENTATION:

Herein, we present the case of a 73-year-old woman with a complex medical history, including possible eosinophilic granulomatosus with polyangiitis, who was admitted to the medical intensive care unit at Dartmouth-Hitchcock Medical Center and was found to have concominant SSAH and SSDH.

CONCLUSION:

This case serves as a review of the common and uncommon symptoms of IS-SAH with SSDH to improve recognition of this acute neurosurgical emergency in the medical intensive care setting.
Patterns of single limb forces during terrestrial and arboreal locomotion in rosy-faced lovebirds (Psittaciformes: Agapornis roseicollis)
Authors: Young Melody W. , Lynch Samantha K. , Dickinson Edwin , Currier Allen A. , Davoli Elizabeth C. , Hanna Christopher S. , Fischer Hannah M. , DiUbaldi Gianluca A. , Granatosky Michael C.
Published: July 2022

The biomechanical demands of arboreal locomotion are generally thought to necessitate specialized kinetic and kinematic gait characteristics. While such data have been widely collected across arboreal quadrupeds, no study has yet explored how arboreal substrates influence the locomotor behavior of birds. Parrots – an ancient arboreal lineage that exhibit numerous anatomical specializations towards life in the trees – represent an ideal model group within which to examine this relationship. Here, we quantifiy limb loading patterns within the rosy-faced lovebird (Agapornis roseicollis) across a range of experimental conditions to define the circumstances under which arboreal gaits are triggered, and how, during arboreal walking, gait patterns change across substrates of varying diameter. In so doing, we address longstanding questions as to how the challenges associated with arboreality affect gait parameters. Arboreal locomotion was associated with the adoption of a sidling gait, which was employed exclusively on the small and medium diameter poles but not terrestrially. When sidling, the hindlimbs are decoupled into a distinct leading limb (which imparts exclusively braking forces) and trailing limb (which generates only propulsive forces). Sidling was also associated with relatively low pitching forces, even on the smallest substrate. Indeed, these forces were significantly lower than mediolateral forces experienced during striding on terrestrial and large diameter substrates. We propose that the adoption of sidling gaits is a consequence of avian foot morphology and represents a novel form of arboreal locomotion where inversion/eversion is impossible. Such movement mechanics is likely widespread among avian taxa and may also typify patterns of arboreal locomotion in humans.
Susceptibility-weighted imaging: an emerging technique for evaluation of the spine and spinal cord
Authors: Sharma Shalini , Neelavalli Jaladhar , Shah Tejas , Gupta Rakesh Kumar
Published: July 2022

We present the application of three-dimensional susceptibility-weighted imaging technique for evaluation of pathologies of the spine and spinal cord. This work focuses on the advantage of this imaging technique as an adjunct to the conventional imaging to evaluate various disorders of the spine and spinal cord like trauma, degenerative diseases, vascular malformations, and tumours, where susceptibility-weighted imaging may offer valuable harmonising evidence that may be helpful in the diagnosis and management of the patients with these pathologies.
Διαπολιτισμική επικοινωνία στο πλαίσιο της εκπαίδευσης ενηλίκων
Authors: Γιακουμάκης Γεώργιος
Published: July 2022

Αντικείμενο της παρούσας διδακτορικής διατριβής είναι η μελέτη του λόγου των ενήλικων εκπαιδευόμενων και εκπαιδευτριών και η κατασκευή ταυτοτήτων στις κοινότητες πρακτικής των τμημάτων δια βίου μάθησης, στο πλαίσιο της εκπαίδευσής ενηλίκων. Σημεία εστίασης αποτελούν η οργάνωση της συνομιλίας και τα γλωσσικά φαινόμενα της εναλλαγής κώδικα, της διαγλωσσικότητας, της ευγένειας/αγένειας και του χιούμορ. Οι στόχοι της έρευνας καθορίστηκαν ως εξής: πρώτον, να μελετηθεί η δομή της συνομιλίας και η κατανομή συνομιλιακών δικαιωμάτων στη ενήλικη διδακτική διεπίδραση. Δεύτερον, να καταγραφεί ο λόγος των ενήλικων εκπαιδευομένων στα τμήματα δια βίου μάθησης, κατά τη διάρκεια της διδασκαλίας ξένων γλωσσών. Τρίτον, να μελετηθούν τα φαινόμενα που εμφανίζονται κατά την επικοινωνία των συμμετεχουσών και ιδιαίτερα η εναλλαγή κώδικα, η διαγλωσσικότητα, η α/ευγένεια και το χιούμορ. Τέλος, να μελετηθεί η κατασκευή ταυτοτήτων μέσω του λόγου και δια των υπό διερεύνηση γλωσσικών φαινομένων. Ερευνητικά εργαλεία συλλογής των δεδομένων για την όσο το δυνατόν περισσότερο ρεαλιστική καταγραφή της πραγματικότητας στην αίθουσα διδασκαλίας αποτέλεσαν: α. η μέθοδος της συμμετοχικής παρατήρησης, β. η ψηφιακή ηχογράφηση του αυθόρμητου και φυσικού λόγου και γ. το ερωτηματολόγιο με σκοπό τη συλλογή βιογραφικών στοιχείων των συμμετεχόντων. Η συστηματική ανάλυση φυσικού ηχογραφημένου λόγου με χρήση πρωτογενούς υλικού, καθώς και η εξέταση των εκφωνημάτων σε πραγματικές επικοινωνιακές περιστάσεις, οδήγησε στην επιλογή λογοαναλυτικών προσεγγίσεων της συνομιλιακής οργάνωσης και των συνομιλιακών πρακτικών. Αξιοποιούνται μεθοδολογικά εργαλεία της ανάλυσης συνομιλίας, καθώς και θεωρητικές αρχές από την κοινωνιογλωσσολογική προσέγγιση της διαπολιτισμικής επικοινωνίας και την προσέγγιση της κοινωνικής κατασκευής της ταυτότητας στη συνομιλιακή διεπίδραση. Όλα τα γλωσσικά φαινόμενα που εξετάζουμε εν τέλει ανάγονται σε συνομιλιακές στρατηγικές διαχείρισης της επικοινωνίας και διαχείρισης του προσώπου κατά τη διεπίδραση, τόσο από την πλευρά του συστήματος οργάνωσης της συνομιλίας όσο και από τις πρακτικές που εφαρμόζουν οι συμμετέχουσες στην οργάνωση των συνεισφορών τους και στην οικοδόμηση των ταυτοτήτων τους. Η ανάλυση των δεδομένων έδειξε ότι η μορφή του συστήματος της οργάνωσης του λόγου στη διδακτική διεπίδραση προσιδιάζει σε ένα περισσότερο συμμετρικό σύστημα συνομιλίας, συμβάλλοντας στην εγκαθίδρυσή μιας λιγότερο ασύμμετρης σχέσης μεταξύ των μερών και στην απομειώση της κοινωνικής απόστασης των αντιθετικών ταυτοτήτων εκπαιδεύτριας - εκπαιδευόμενων. Φαίνεται ότι η γνωσιακή/διδακτική διαδικασία είναι δευτερεύουσα σε σχέση με την κοινωνική διεπίδραση και την οικοδόμηση ενδοομαδικών δεσμών εγγύτητας στους οποίους συμμετέχουν τόσο οι εκπαιδευόμενες όσο και οι εκπαιδεύτριες. Σημαντικές επίσης, είναι, οι εθνοπολιτισμικές ταυτότητες που κατασκευάζονται στον λόγο των εκπαιδευτριών και των εκπαιδευόμενων, καθώς και οι ταυτότητες που σχετίζονται με την ηλικιακή απόσταση των συμμετεχουσών στις κοινότητες πρακτικής των τμημάτων δια βίου μάθησης.
Δεοντοκρατική, ωφελιμιστική, θεολογική συζήτηση για τη δωρεά οργάνων
Authors: Καραματζιάνη Ελένη
Published: July 2022

Η παρούσα διατριβή είναι μια συζήτηση για το θέμα των μεταμοσχεύσεων πουαναδύει φως πολλών εννοιών. Αρχικά επιχειρείται μια σύντομη ιστορικήεπισκόπηση αναφορικά με τη Δωρεά Οργάνων από τον Όμηρο και τη ΧριστιανικήΠαράδοση μέχρι τη σύγχρονη πραγματικότητα. Ακολούθως, εξετάζoνται οιμείζονες ηθικές παραδόσεις και ο τρόπος κατά τον οποίον αυτές εγκύπτουνστην δωρεά οργάνων, με ιδιαίτερη εστίαση σε θεωρίες αυτονομίας(δεοντοκρατική προσέγγιση), αλλά και σε θεωρίες αλληλεγγύης καιφιλανθρωπίας (ωφελιμιστική και θεολογική προσέγγιση αντιστοίχως),αναφέροντας παράλληλα την εμπορία οργάνων, ζήτημα που συζητάται,εστιάζοντας κυρίως στο επιχείρημα από τη δυνατότητα. Παράλληλα, εκθέτονταςτις απόψεις των Immanuel Kant, Julian Savulescou, Herman TristramEngelhardt, John Harris, Charlies Erin και Peter Singer επιχειρείται ηαποτύπωση των θέσεων που έχουν διατυπωθεί υπέρ και κατά της ηθικήςεπιτακτικότητας της δωρεάς οργάνων, καθώς και η εμπορευσιμότητα τωνμοσχευμάτων. Ενώ, χρησιμοποιείται προσφυώς το επιχείρημα της ολισθηρήςπλαγιάς, ώστε να καταδειχθούν οι δυνητικοί κίνδυνοι, ενώ παράλληλασυζητούνται εκτενώς ζητήματα που άπτονται της ενήμερης συναίνεσης, αλλάκαι της εικαζόμενης συγκατάθεσης. Τέλος, εξετάζονται τα ΑποτελέσματαΈρευνας Ερωτηματολογίου σχετικά με τη Δωρεά Οργάνων βασισμένα στιςΔεοντοκρατικές, Ωφελιμιστικές και Θεολογικές Προσεγγίσεις, ενώπραγματοποιείται παραλληλισμός της αλληγορίας του σπηλαίου του Πλάτωνα, μετην άγνοια και τις προκαταλήψεις των ανθρώπων, βάση των οποίωνδικαιολογείται η άρνησή τους να προβούν σε μεταμοσχευτικές διαδικασίες.
Κεντητοί επιτάφιοι Αγίου Όρους (14ος-18ος αι.)
Authors: Τουτός Νικόλαος
Published: July 2022

Αντικείμενο μελέτης της εργασίας είναι οι κεντητοί επιτάφιοι του Αγίου Όρους που εντάσσονται χρονικά από τις αρχές του 14ου έως και τα τέλη του 18ου αι. Εξετάζονται συνολικά τριάντα επιτάφιοι που χρονολογούνται από τις απαρχές της εμφάνισης του συγκεκριμένου αυτού λειτουργικού πέπλου στα χρόνια της βασιλείας του Ανδρονίκου Β΄ Παλαιολόγου (1282-1328) μέχρι και τα τέλη του 18ου αι., λίγο πριν την εμφάνιση και εξάπλωση των ρωσικών χρυσοκέντητων επιταφίων που θα κατακλύσουν την Ορθόδοξη Ανατολή μετά τα μέσα του 19ου αι. Αρχικά, ανιχνεύονται οι ιστορικο-θεολογικές και λειτουργικές προϋποθέσεις που οδήγησαν στην εμφάνιση του κεντητού αυτού πέπλου με την απεικόνιση του Χριστού-Αμνού, ενώ ακολούθως εξετάζεται ο τρόπος της χρήσης του επιταφίου στην περίοδο πριν την Άλωση της Κωνσταντινούπολης (1453) αξιοποιώντας τις πηγές, τα λειτουργικά κείμενα της εποχής αυτής σε συνδυασμό με τις εντοίχιες μνημειακές απεικονίσεις της Ουράνιας Λειτουργίας. Στη συνέχεια διερευνάται η τυπολογική εξέλιξη των επιταφίων και ο τρόπος με τον οποίο έγινε η μετάβαση από τον πρώιμο λειτουργικό-συμβολικό τύπο (Μέγας Αήρ) του ύστερου Βυζαντίου στον αμιγώς ιστορικό-αφηγηματικό τύπο από το β΄ μισό του 17ου αι. και εξής, με τη μεσολάβηση όμως του λεγόμενου μεταβατικού - συγχωνευμένου τύπου στο μεσοδιάστημα των δύο αυτών περιόδων (15ος-αρχές 17ου αι.). Γίνεται ιδιαίτερη αναφορά στις λειτουργικές επιγραφές που συνοδεύουν τους κεντητούς επιταφίους, αλλά και στους επώνυμους κτίτορες-αφιερωτές τους. Το τρίτο μέρος της εργασίας συγκροτεί ο πολυσέλιδος αναλυτικός κατάλογος των τριάντα αθωνικών επιταφίων. Στο λήμμα του κάθε επιταφίου δίνονται γενικές πληροφορίες, μεταγράφονται και σχολιάζονται οι επιγραφές (λειτουργικές και αφιερωματικές) και, εκτός από την τυπολογική την εικονογραφική ανάλυση του επιταφίου, εξετάζονται οι τεχνικές και τα υλικά κατασκευής των τριάντα χρυσοκεντημάτων. Η εργασία ολοκληρώνεται με την παράθεση, εν είδει πολυσέλιδου Παραρτήματος, των χειρόγραφων Τυπικών και Τριωδίων από τις από τις μοναστηριακές συλλογές του Αγίου Όρους, στα οποία αποτυπώνεται η μαρτυρία της αθωνικής χειρόγραφης παράδοσης αναφορικά με τη χρήση του κεντητού αέρα-επιταφίου στο Τυπικό του Μεγάλου Σαββάτου. Ειδικότερα, μέσα από τις αναλυτικές μεταγραφές χειρόγραφων Τυπικών και Τριωδίων δημοσιεύεται ένα άγνωστο και αδιερεύνητο υλικό που αξιοποιήθηκε με τον καλύτερο τρόπο σχετικά με την εμφάνιση και σταδιακή αλλαγή στην τελετουργική χρήση του αέρα-επιταφίου στους χρόνους πριν και αμέσως μετά την Άλωση.
Solidarity in the EU after 2020; prospects for the cohesion “policy space"
Authors: Andreou George
Published: July 2022

In 2020, the EU established the “Recovery and Resilience Facility” (RRF), which aims at promoting its economic, social, and territorial cohesion. This development has had a significant impact on the institutional architecture of cohesion policy, which is widely viewed as the main “EU solidarity tool”. The goal of this paper is to map the institutional configuration of “old” and “new” funding programs devoted to the promotion of cohesion and solidarity in Europe through the lenses of public policy analysis and historical institutionalism. It is argued that despite its impressive redistributive impact in territorial terms, the new Facility does not represent a break from the past when it comes to the quality of its solidarity content. On top of that, by adding RRF in a “cohesion policy space” burdened with old and new policy goals and means and lacking a clear territorial and social focus, EU actors have further undermined both the coherence and the solidarity impact of “old” cohesion policy.
Health and Economic Responses to the COVID-19 Crisis in the EU
Authors: Katsikas Dimitris
Published: July 2022

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Foreword
Authors: Ribeiro Hoffmann Andrea , Katsikas Dimitris
Published: July 2022

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Records of antibodies in breast milk in postpartum women who have been vaccinated or exposed to COVID-19: A systematic review
Authors: Mardiyan Kurniawati Eighty , Rahmawati Nur Anisah
Published: July 2022

Background: Breast milk is a critical element in developing a baby's immunity through immune transfer. Antibodies are an essential unit of immunity against infection with the SARS-CoV-2 virus. This paper explores antibodies in breast milk in postpartum women who have been vaccinated or exposed to coronavirus disease 2019 (COVID-19). Duration of antibody appearance was studied to determine the adequate time in transferring antibodies by breastfeeding.

Methods: Three databases, PubMed, Google Scholar, and ScienceDirect, were used as sources of articles. Inclusion criteria applied in selecting articles were prospective observational study or experimental design study in English, evaluating antibodies in breast milk, and conducted between 2019–2021. Article quality and risk of bias were assessed with Critical Appraisal Skills Programme (CASP). The data found were synthesized in a narrative manner.

Results: This systematic review included 20 articles. A total of 306 postpartum women who were infected with COVID-19, 20 postpartum women who had viral symptoms and 495 postpartum women who had been vaccinated were studied. Immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies were found in the breast milk of infected and vaccinated postpartum women. SARS CoV-2 infection is associated with the presence of IgA dominant, whereas vaccination is related to the presence of IgG dominant. Antibodies persisted from day 10 of onset to 10 months in infected postpartum women and started from three days to six weeks in vaccinated postpartum women. Meta-analysis could not be carried out due to the variety of articles.

Conclusions: Antibodies found in breast milk in infected and vaccinated postpartum women have different dominant types. Further research needs to be done regarding the mechanism of antibody transfer in breast milk, longer research duration and studies that directly examine the comparison of antibodies in breast milk in vaccinated and infected postpartum women.

Registration: PROSPERO (CRD42022340859, 23 June 2022).
Effects of concomitant benzodiazepines and antidepressants long-term use on perspective-taking
Authors: Gonçalves Ana R. , Soares Márcia , Garcez Helena , Fernandes Carina , Pereira Mariana R. , Silveira Celeste , Marques-Teixeira João , Barbosa Fernando
Published: July 2022

Background: Benzodiazepines and antidepressants are known to alter responses to empathic pain when used alone, however the effects of their combined use on empathy are unknown. In order to examine the effects of concomitant benzodiazepines and antidepressants long-term use on perspective-taking, we analyzed behavioral and neural changes on perspective-taking ability using event-related potentials.

Methods: To this purpose, 13 long-term concomitant benzodiazepines and antidepressants users and 13 healthy controls performed a task designed to assess affective perspective-taking with simultaneous EEG recording.

Results: The behavioral results revealed similar performance between groups. The neural results showed no significant differences between groups for the N170 and late positive potential (LPP) components. These results seem to suggest that long-term use of benzodiazepines and antidepressants together does not affect perspective-taking abilities nor the processing of related information.

Conclusions: The concomitant benzodiazepines and antidepressants long-term use seem to preserve the perspective-taking ability of social cognition.
MEANING AND ROLE OF HIGHER SCHOOL FOR BULGARIAN REGIONS’ SUSTAINABLE DEVELOPMENT
Authors: Berberova-Valcheva Tsvetelina , Petrov Kamen , Tzonkov Nikolai
Published: July 2022

This article is a partial picture of higher education in Bulgaria and the need to provide quality educational services at the regional level. The exhibition presents the importance and role of higher education in the recruitment of the regional elite and the quality of staff. A methodological framework that is proposed, assumes the systematization of the accumulated theoretical and practical experience in the field of higher education. Some deficiencies have been systematized, which affect the quality of education and the mismatch between business expectations at the level of planning regions, as well as the impact on municipalities and the need for staff for higher education graduates in the country. The main trends and conditions in which higher education in Bulgaria is developing and the search for opportunities to increase its role within the Bulgarian regions are outlined. In practice, regional development can become a national priority by building a quality educational environment and having a balanced regional development.
BLOCKCHAIN AND THE APPLICATION OF BLOCKCHAIN TECHNOLOGY
Authors: Cekerevac Zoran , Cekerevac Petar
Published: July 2022

Thanks to cryptocurrencies, blockchain technology has recently become the focus of interest of both scientists and the public. The paper presents the principles on which blockchain is based and analyzes the possibilities of the application of blockchain technology in more detail. In a part of the analysis, some of the most common cryptocurrencies were considered, such as Bitcoin, Ethereum, USD Coin, Ripple, and ADA. Blockchains' applicability in supply chains, finance, real estate, health care, voting, and smart cities the authors discussed in the chapter about the application of blockchain technology. A separate chapter discusses the limitations of blockchain technology. Based on the analysis, at the end of the paper, the authors conclude that blockchain technology has great potential in automating and optimizing business processes and protecting the information and privacy of users. Given the demonstrated interest of companies in blockchain technology, investments, and demand for staff who understand it and can create applications, the authors concluded that the time of blockchain is yet to come.
GREEN LOANS AND ECO LOANS IN THE BANKING SECTOR UNDER THE CONDITIONS OF CLIMATE CHANGES
Authors: Ciobu Stela , Sajin Teodora
Published: July 2022

Environmental pollution and depletion of non-renewable resources are major problems at the international, national, regional, and local levels that endanger biological diversity, human health, and the further development of the economy. The economic and financial impact of climate change can lead to considerable future losses for banking financial institutions and environmental degradation is becoming a crucial dilemma globally. Climate change has become a threat to sustainable development. Mitigation and adaptation should be a priority of national policy. The increasing tendency of excessive consumption of natural resources due to intensive economic activity of corporations has led to a significant increase in greenhouse gas emissions, leading to global warming and, ultimately, to global climate change. These issues present the scientific community with the dilemma of finding a compromise between economic development and its impact on climate change. The main aim of the research is to emphasize the importance of responsible lending by promoting green banking products as green and eco loans, identify the potential environmental impact of negligent financing under the conditions of climate change, give definitions of this topic, consider current trends in the banking sector and prospects of development. Also, based on international experience, the authors tried to identify the main aspects of the environmental management policy of ProCredit Group and examine the share of the green loan portfolio at the group level, determine the environmental impact and subtract the improvement strategies and methods for implementing green lending in the Republic of Moldova.
FEATURES OF CHANGE MANAGEMENT IN SMEs IN THE ERA OF INFORMATIZATION OF SOCIETY
Authors: Dorogaia Irina
Published: July 2022

The trends of recent years of business development testify to the cardinal changes that have swallowed up companies. Small and medium enterprises face serious challenges related to informatization and digitalization. Considering the specifics of this business area, one can say that organizational changes in such companies will be significantly different. This article deals with the problems associated with changing environmental conditions, namely, digitalization, and possible options for overcoming the existing difficulties. The SME sector is facing serious challenges in the crisis. In addition to the external factors faced by all national companies, the overall digitalization has a significant impact, which affects an increasing number of companies in the Republic of Moldova and worldwide. This study aims to answer a multifaceted question: What are the features of the enterprises' adaptation in this sector to modern provocations? Many contemporary authors consider the conceptual characteristics of this issue. The topic of the digitalization of society is very relevant, but the authors pay little attention to small and medium enterprises. Given their importance in the Moldovan economy, we analyzed the specifics of the transition of this sector of the Moldovan economy.
INNOVATIVE STARTUPS AND LOCAL DEVELOPMENT - THE CASE OF THE NORTH-WEST REGION OF ROMANIA
Authors: Gavrilut Darie , Badulescu Alina , Badulescu Daniel
Published: July 2022

This article analyses the role and importance of new startups, especially innovative ones, in the regional context. What is more, we aim to assess the impact of measures at the local government level, meant to improve the business environment. In practical terms, we have addressed the situation of the startup sector in a developing region of Romania, namely the North-West Region, and, in two important cities located in this area - Cluj-Napoca and Oradea. The theoretical part has the task of discussing the main theories and contributions regarding aspects of the business environment and future opportunities that shape the creation of startups and how they will develop. At the same time, the theoretical part deals with finding and analyzing the factors that influence startup creation. Based on the theoretical foundations, a comparison between two selected areas has been made numerically (using quantitative comparison), and from the perspective of the future impact that innovative, fast-growth-oriented startups can have on the economic and social dynamization of the cities and region in which they operate. Finally, we have analyzed the extent to which the development of this sector can influence and support the creation of a dynamic entrepreneurial ecosystem, a solid infrastructure, that supports medium and long-term economic growth in these areas.
COOT OPTIMIZATION ALGORITHM FOR PARAMETER ESTIMATION OF PHOTOVOLTAIC MODEL
Authors: Rushdi Hussam , Al-Naima Fawzi
Published: July 2022

Because of the technical and environmental advantages of many solar energy sources, their use has recently been rapidly rising. The extraction of the unknown parameters in photovoltaic models is one of the key challenges in the modeling and simulation of solar energy sources. To satisfy the behavior of the solar photovoltaic (SPV) cells, the Single-Diode Model (SDM) is recommended as a more dependable modeling method. In this study, we applied the recently introduced meta-heuristic optimization method that inspires the behavior of the swarm of birds called Coot. This Coot Algorithm is used to estimate the unknown parameters of an SPV cell/module at 33 ºC. Simulation results of this study were compared with other nine different previous Optimization Algorithms, which are Moth Flame Optimization (MFO), Dragonfly Algorithm (DA), Whale Optimization Algorithm (WOA), Grey Wolf Optimization (GWO), Ant Lion Optimization (ALO), Harris Hawk Optimization (HHO), Hybrid of Particle Swarm Optimization and Grey Wolf Optimization (PSOGWO), Marine Predator Algorithm (MPA), and African Vultures Optimization Algorithm (AVOA). The obtained results of this comparison showed that the Coot algorithm outperformed the previous algorithms in terms of the root mean square error (RMSE) and the degree of convergence between the power versus voltage curve and the current versus voltage curve compared with the measured data. Moreover, the results confirm that the Coot optimization algorithm is favorable in reducing time and improving accuracy.
ANALYSIS OF THE STATUS OF THE FRAUD WITHIN THE ENTERPRISES IN DIFFERENT COUNTRIES AROUND THE WORLD
Authors: Rybalchenko Lyudmila , Ryzhkov Eduard
Published: July 2022

The consequences of fraudulent actions are losses of significant financial resources in companies, which endangers their business. The resolution of the occurred criminal issues is complex and therefore requires the exchange of the experience of many countries worldwide. It is needed to conduct an internal audit to detect embezzlement in the company. The relevant department must carry out the audit, e.g., the security services department, involving an internal audit, internal control, and legal department. Identification of persons possibly involved in fraudulent actions is a complex issue that carries a criminal responsibility. Different companies impose disciplinary liability, penalties, or criminal liability on employees. The most common are corruption, misappropriation of property, and falsification of financial statements. The variety of fraudulent schemes grows and becomes even more dangerous for businesses. Fraudsters analyze the financial statements of enterprises and find vulnerabilities to create threats and fraudulent actions. Such schemes lead to risks for both large and small businesses worldwide. Most companies have been affected by fraud incidents in recent years. The unreliable partners frequently cause losses in the companies. The negative consequences of risky activities include losses of money, reputation, and trust of customers and partners. All affect the loss of profits, which is the purpose of the activities.
PARTICIPATORY BUDGETING IN DABROWA GORNICZA - A RESEARCH REPORT
Authors: Sroka Jacek , Pawlica Beata
Published: July 2022

This article contains the preliminary results of our research on participatory budgeting in Poland. We did it as part of the research project No. 2019/33/B/HS5/00353 financed by the National Science Center under the OPUS 17 program "Evolution of the participatory budgeting in Poland - towards a deliberation or plebiscite?" The goal of the research was to find out what opinions about participatory budgeting the inhabitants of Dabrowa Gornicza held. In Poland, the participatory budget is called partycypacyjny bużet. The participatory budget is a democratic process in which residents can co-determine public spending in the city in the next fiscal year. In Poland, participatory budgets have been developing for a decade. Until 2018, the decision to introduce a civic budget belonged to local governments. Therefore, although they grew out of one idea, the different variants of budgeting differed among themselves, both in procedural and practical dimensions. In 2018, the law was amended, which introduced the regulation of participatory budgets into the common law.