bluuebunny/crossref_metadata_2025 huggingface dataset:
Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine
Authors: Corral Paulina , Amoozegar Mohammad A. , Ventosa Antonio
Published: January 2020

The organisms thriving under extreme conditions better than any other organism living on Earth, fascinate by their hostile growing parameters, physiological features, and their production of valuable bioactive metabolites. This is the case of microorganisms (bacteria, archaea, and fungi) that grow optimally at high salinities and are able to produce biomolecules of pharmaceutical interest for therapeutic applications. As along as the microbiota is being approached by massive sequencing, novel insights are revealing the environmental conditions on which the compounds are produced in the microbial community without more stress than sharing the same substratum with their peers, the salt. In this review are reported the molecules described and produced by halophilic microorganisms with a spectrum of action in vitro: antimicrobial and anticancer. The action mechanisms of these molecules, the urgent need to introduce alternative lead compounds and the current aspects on the exploitation and its limitations are discussed.
Reconstruction for Defects of Total Nail Bed and Germinal Matrix Loss with Acellular Dermal Matrix Coverage and Subsequently Skin Graft
Authors: Liu Tsung-Hsien , Hsieh Meng-Chien , Chou Ping-Ruey , Huang Shu-Hung
Published: January 2020

Background and Objectives: Nail bed and germinal matrix loss due to wide excision for fingertip tumors or malignancy are occasionally encountered complications. These defects also result from severely comminuted fingertip crush injuries. Large-area dorsal finger or toenail bed defects, which usually present with phalangeal bone exposure, remain challenging regardless of the usage of different reconstruction strategies. This study aimed to evaluate the clinical outcome of a staged operation with an acellular dermal matrix coverage and subsequent skin graft as reconstruction for defects of total nail bed, germinal matrix loss, and bone exposure. Materials and Methods: From April 2018 to October 2019, four patients with total nail bed, germinal matrix, and bone exposure loss after surgery were enrolled in our series. A staged operation of the acellular dermal matrix coverage with subsequent skin graft was performed on these patients. Skin graft take rate, oncological prognosis, and cosmetic outcome were evaluated. Patients were followed up for 5–13 months. An excellent skin graft take rate with a satisfying aesthetic result without local malignancy recurrence was noted. Minimal functional deficit and donor site morbidity were reported. Results: A staged operation with acellular dermal matrix coverage and subsequent skin graft proves to serve as a feasible strategy for patients who experience total nail bed, germinal matrix loss, and bone exposure after surgery. Conclusions: This reconstruction method provides a reliable repair result, satisfying aesthetic outcomes, as well as having minimal functional deficits and donor site morbidity.
Focused Electron Beam-Based 3D Nanoprinting for Scanning Probe Microscopy: A Review
Authors: Plank Harald , Winkler Robert , Schwalb Christian H. , Hütner Johanna , Fowlkes Jason D. , Rack Philip D. , Utke Ivo , Huth Michael
Published: January 2020

Scanning probe microscopy (SPM) has become an essential surface characterization technique in research and development. By concept, SPM performance crucially depends on the quality of the nano-probe element, in particular, the apex radius. Now, with the development of advanced SPM modes beyond morphology mapping, new challenges have emerged regarding the design, morphology, function, and reliability of nano-probes. To tackle these challenges, versatile fabrication methods for precise nano-fabrication are needed. Aside from well-established technologies for SPM nano-probe fabrication, focused electron beam-induced deposition (FEBID) has become increasingly relevant in recent years, with the demonstration of controlled 3D nanoscale deposition and tailored deposit chemistry. Moreover, FEBID is compatible with practically any given surface morphology. In this review article, we introduce the technology, with a focus on the most relevant demands (shapes, feature size, materials and functionalities, substrate demands, and scalability), discuss the opportunities and challenges, and rationalize how those can be useful for advanced SPM applications. As will be shown, FEBID is an ideal tool for fabrication/modification and rapid prototyping of SPM-tipswith the potential to scale up industrially relevant manufacturing.
An Integrated Optofluidic Platform Enabling Total Phosphorus On-Chip Digestion and Online Real-Time Detection
Authors: Li Chang , Wang Bingbing , Wan Hao , He Rongxiang , Li Qi , Yang Siyuan , Dai Wencan , Wang Ning
Published: January 2020

This paper presents a total phosphorus online real-time monitoring system integrated with on-chip digestion based on the merits of optofluidic technology. The integrated optofluidic device contains a hollow optical fiber employed for pretreatment and digestion of phosphorus solution samples, a polydimethylsiloxane (PDMS)-based micromixer with convergent–divergent walls designed to enable sufficient mixing and chromogenic reaction, and a couple of optical fiber collimators attached with a Z-shaped flow cell for optical detection. Details of system design and fabrication are introduced in this paper. In the experiment, on-chip digestion of four typical phosphates in aqueous solution including organophosphorus and inorganic phosphorus is investigated under different reaction conditions, such as digestion temperature, concentration of oxidant and pH value, and the optimal reaction parameters are explored under different conditions. Meanwhile, we demonstrate the online real-time monitoring function of the optofluidic device, and the digestion mechanisms of four different phosphates are analyzed and discussed. Compared with the national standard method, we find that the measurement accuracy and sensitivity are acceptable when the concentration of total phosphorus is between 0.005–0.9 mg/L (by weight of P) in aqueous solution, which covers the range defined in the national standard. The traditional digestion time of several hours is greatly reduced to less than 10 s, and the content of total phosphorus can be obtained in a few minutes. The integrated optofluidic device can significantly shorten the test time and reduce the sample amount, and also provides a versatile platform for the real-time detection and analysis of many biochemical samples.
Comparison of Anthraquinones, Iridoid Glycosides and Triterpenoids in Morinda officinalis and Morinda citrifolia Using UPLC/Q-TOF-MS and Multivariate Statistical Analysis
Authors: Wang Maoyuan , Wang Qinglong , Yang Qing , Yan Xiaoxia , Feng Shixiu , Wang Zhunian
Published: January 2020

Roots of Morinda officinalis and Morinda citrifolia have been interchangeably used in traditional Chinese medicine. However, there is no experimental evidence to support this. In this study, a ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS)-based approach and a multivariate statistical analysis (MSA) were adopted to compare the difference in the chemical compounds present in the root extract of M. officinalis and M. citrifolia. There were 26 anthraquinones, 15 triterpenes, and 8 iridoid glycosides identified in the root extracts of M. officinalis, 30 anthraquinones, 1 triterpene, and 8 iridoid glycosides in the root extracts of M. citrifolia. Among these, 25 compounds presented in both plants. In addition, a principal component analysis (PCA) showed that these two herbs could be separated clearly. Furthermore, an orthogonal partial least squares-discriminant analysis (OPLS-DA) found 9 components that could be used as chemical markers to discrimination the root extracts of M. officinalis and M. citrifolia. In addition, the results of a Cell Counting Kit 8 (CCK-8) assay and cell colony formation assay indicated that methanol root extracts of M. officinalis and M. citrifolia showed no cell cytotoxicity to normal cells, even promoted the proliferation of normal liver cells. To our knowledge, this is the first time that the differences between the root extracts of M. officinalis and M. citrifolia (Hainan province) have been observed systematically at the chemistry level.
DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)
Authors: Zhao Lei , Zhao Xiaoyan , Ma Yue , Zhang Yan , Wang Dan
Published: January 2020

Yam yellow pigments (YP) are natural pigments formed during the storage of freshly cut yam (Dioscorea opposita) under certain conditions. The interaction of YP with calf thymus DNA (ctDNA) and its protective effect against DNA oxidative damage were investigated using multiple spectroscopic techniques, competitive binding experiments, viscosity measurements, and gel electrophoresis. Results showed that YP participated in intercalative binding with ctDNA. YP exhibited a protective effect against hydroxyl-induced DNA damage, which was attributed to the high hydroxyl radical scavenging activity of YP. Our findings improve our understanding of the mechanism of interaction between YP and ctDNA, and provide a theoretical basis for the application of YP in the food and drug industry.
Potential Beneficial Effects of Vitamin D in Coronary Artery Disease
Authors: Legarth Christian , Grimm Daniela , Krüger Marcus , Infanger Manfred , Wehland Markus
Published: January 2020

Vitamin D plays a pivotal role in bone homeostasis and calcium metabolism. However, recent research has indicated additional beneficial effects of vitamin D on the cardiovascular system. This review aims to elucidate if vitamin D can be used as an add-on treatment in coronary artery disease (CAD). Large-scale epidemiological studies have found a significant inverse association between serum 25(OH)-vitamin D levels and the prevalence of essential hypertension. Likewise, epidemiological data have suggested plasma levels of vitamin D to be inversely correlated to cardiac injury after acute myocardial infarction (MI). Remarkably, in vitro trials have showed that vitamin D can actively suppress the intracellular NF-κB pathway to decrease CAD progression. This is suggested as a mechanistic link to explain how vitamin D may decrease vascular inflammation and atherosclerosis. A review of randomized controlled trials with vitamin D supplementation showed ambiguous results. This may partly be explained by heterogeneous study groups. It is suggested that subgroups of diabetic patients may benefit more from vitamin D supplementation. Moreover, some studies have indicated that calcitriol rather than cholecalciferol exerts more potent beneficial effects on atherosclerosis and CAD. Therefore, further studies are required to clarify these assumptions.
Pasta Consumption and Connected Dietary Habits: Associations with Glucose Control, Adiposity Measures, and Cardiovascular Risk Factors in People with Type 2 Diabetes—TOSCA.IT Study
Authors: Vitale Marilena , Masulli Maria , Rivellese Angela Albarosa , Bonora Enzo , Babini Anna Carla , Sartore Giovanni , Corsi Laura , Buzzetti Raffaella , Citro Giuseppe , Baldassarre Maria Pompea Antonia , Bossi Antonio Carlo , Giordano Carla , Auciello Stefania , Dall’Aglio Elisabetta , Iannarelli Rossella , Tonutti Laura , Sacco Michele , Di Cianni Graziano , Clemente Gennaro , Gregori Giovanna , Grioni Sara , Krogh Vittorio , Riccardi Gabriele , Vaccaro Olga
Published: January 2020

Background: Pasta is a refined carbohydrate with a low glycemic index. Whether pasta shares the metabolic advantages of other low glycemic index foods has not really been investigated. The aim of this study is to document, in people with type-2 diabetes, the consumption of pasta, the connected dietary habits, and the association with glucose control, measures of adiposity, and major cardiovascular risk factors. Methods: We studied 2562 participants. The dietary habits were assessed with the European Prospective Investigation into Cancer and Nutrition (EPIC) questionnaire. Sex-specific quartiles of pasta consumption were created in order to explore the study aims. Results: A higher pasta consumption was associated with a lower intake of proteins, total and saturated fat, cholesterol, added sugar, and fiber. Glucose control, body mass index, prevalence of obesity, and visceral obesity were not significantly different across the quartiles of pasta intake. No relation was found with LDL cholesterol and triglycerides, but there was an inverse relation with HDL-cholesterol. Systolic blood pressure increased with pasta consumption; but this relation was not confirmed after correction for confounders. Conclusions: In people with type-2 diabetes, the consumption of pasta, within the limits recommended for total carbohydrates intake, is not associated with worsening of glucose control, measures of adiposity, and major cardiovascular risk factors.
Synbiotic Combination of Djulis (Chenopodium formosanum) and Lactobacillus acidophilus Inhibits Colon Carcinogenesis in Rats
Authors: Lee Chih-Wei , Chen Hong-Jhang , Chien Yu-Hua , Hsia Shih-Min , Chen Jiann-Hwa , Shih Chun-Kuang
Published: January 2020

Djulis is a functional grain containing prebiotic dietary fiber, which has an anti-cancer potential. This study examined the preventive effect of djulis alone or in combination with Lactobacillus acidophilus on colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) and dextran sulfate sodium (DSS). Rats were divided into five groups and fed B (AIN-93G, blank), C (AIN-93G, control), D (10% djulis), DLA (10% djulis plus 5 × 106 cfu L. acidophilus/g), and DHA (10% djulis plus 5 × 107 cfu L. acidophilus/g) diets, respectively. All rats except for those in group B received three doses of DMH (40 mg/kg) by intraperitoneal injection and 3% DSS in drinking water. After 10 weeks of feeding, the colon was analyzed for precancerous lesions and biomarkers. DMH and DSS treatment induced aberrant crypt foci (ACF), especially in the distal colon. D, DLA, and DHA significantly reduced the numbers of total ACF, sialomucin-producing ACF (SIM-ACF), and mucin-depleted foci (MDF) in the distal colon compared to C. Additionally, DLA and DHA further downregulated the expressions of proliferating cell nuclear antigen (PCNA) and cyclooxygenase-2 (COX-2) and regulated apoptosis-related proteins. These results suggest that synbiotic combination of djulis and L. acidophilus shows the best inhibitory effect on colon carcinogenesis via regulation of proliferative, inflammatory, and apoptotic pathways.
The Kidney–Gut–Muscle Axis in End-Stage Renal Disease is Similarly Represented in Older Adults
Authors: Lustgarten Michael S.
Published: January 2020

Decreased renal function, elevated circulating levels of urea, intestinal levels of urea-degrading bacteria, and gut-derived uremic metabolites are present in end-stage renal disease (ESRD), a cohort that has reduced muscle mass and physical function, and poor muscle composition. This phenotype, defined as the kidney–gut–muscle axis, is similarly represented in older adults that do not have ESRD. The purpose of this short communication is to illuminate these findings, and to propose a strategy that can positively impact the kidney–gut–muscle axis. For example, dietary fiber is fermented by intestinal bacteria, thereby producing the short-chain fatty acids (SCFAs) acetate, propionate, and butyrate, which affect each component of the kidney–gut–muscle axis. Accordingly, a high-fiber diet may be an important approach for improving the kidney–gut–muscle axis in ESRD and in older adults that do not have ESRD.
Antidiabetic Properties of Curcumin I: Evidence from In Vitro Studies
Authors: Den Hartogh Danja J. , Gabriel Alessandra , Tsiani Evangelia
Published: January 2020

Type 2 diabetes mellitus (T2DM) is a growing metabolic disease characterized by insulin resistance and hyperglycemia. Current preventative and treatment strategies for T2DM and insulin resistance lack in efficacy resulting in the need for new approaches to prevent and manage/treat the disease better. In recent years, epidemiological studies have suggested that diets rich in fruits and vegetables have beneficial health effects including protection against insulin resistance and T2DM. Curcumin, a polyphenol found in turmeric, and curcuminoids have been reported to have antioxidant, anti-inflammatory, hepatoprotective, nephroprotective, neuroprotective, immunomodulatory and antidiabetic properties. The current review (I of II) summarizes the existing in vitro studies examining the antidiabetic effects of curcumin, while a second (II of II) review summarizes evidence from existing in vivo animal studies and clinical trials focusing on curcumin’s antidiabetic properties.
Bariatric Surgery and Brain Health—A Longitudinal Observational Study Investigating the Effect of Surgery on Cognitive Function and Gray Matter Volume
Authors: Prehn Kristin , Profitlich Thorge , Rangus Ida , Heßler Sebastian , Witte A. Veronica , Grittner Ulrike , Ordemann Jürgen , Flöel Agnes
Published: January 2020

Dietary modifications leading to weight loss have been suggested as a means to improve brain health. In morbid obesity, bariatric surgery (BARS)—including different procedures, such as vertical sleeve gastrectomy (VSG), gastric banding (GB), or Roux-en-Y gastric bypass (RYGB) surgery—is performed to induce rapid weight loss. Combining reduced food intake and malabsorption of nutrients, RYGB might be most effective, but requires life-long follow-up treatment. Here, we tested 40 patients before and six months after surgery (BARS group) using a neuropsychological test battery and compared them with a waiting list control group. Subsamples of both groups underwent structural MRI and were examined for differences between surgical procedures. No substantial differences between BARS and control group emerged with regard to cognition. However, larger gray matter volume in fronto-temporal brain areas accompanied by smaller volume in the ventral striatum was seen in the BARS group compared to controls. RYGB patients compared to patients with restrictive treatment alone (VSG/GB) had higher weight loss, but did not benefit more in cognitive outcomes. In sum, the data of our study suggest that BARS might lead to brain structure reorganization at long-term follow-up, while the type of surgical procedure does not differentially modulate cognitive performance.
Associations between Dietary Patterns and Cardiometabolic Risks in Japan: A Cross-Sectional Study from the Fukushima Health Management Survey, 2011–2015
Authors: Ma Enbo , Ohira Tetsuya , Sakai Akira , Yasumura Seiji , Takahashi Atsushi , Kazama Junichiro , Shimabukuro Michio , Nakano Hironori , Okazaki Kanako , Maeda Masaharu , Yabe Hirooki , Suzuki Yuriko , Kamiya Kenji
Published: January 2020

Cardiometabolic risks were increasing in Fukushima residents after the Great East Japan Earthquake. We examined the association between dietary patterns and cardiometabolic risks in those aged ≥16 years. Dietary patterns were derived by principal component analysis for participants who underwent at least one diet assessment using a short-form food frequency questionnaire during 2011–2013 and a health checkup in 2014 and 2015 (n = 15,409 and 14,999, respectively). In 2014, the adjusted prevalence ratio (PR) and 95% confidence interval (CI) in the highest versus lowest quartile of accumulative mean scores were 0.97 (0.96–0.99) for overweight/obesity, 0.96 (0.95–0.97) for total cholesterol (TC) ≥ 220 mg/dL, 0.96 (0.95–0.98) for low-density lipoprotein cholesterol (LDL-C) ≥ 140 mg/dL, and 0.97 (0.96–0.99) for triglycerides ≥ 150 mg/dL for a vegetable diet and 1.03 (1.01–1.04) for TC ≥ 220 mg/dL and 1.02 (1.01–1.04) for LDL-C ≥ 140 mg/dL for a juice/milk diet. In 2015, we found consistently significant associations for the vegetable and juice/milk diets, and the PR and 95% CI were 0.99 (0.98–1.00) for HDL-C < 40 mg/dL for a meat diet. The continuous promotion of the vegetable pattern diet is necessary to reduce cardiometabolic risks, particularly dyslipidemia, in Japan.
Dietary Patterns in Italy and the Risk of Renal Cell Carcinoma
Authors: Dalmartello Michela , Bravi Francesca , Serraino Diego , Crispo Anna , Ferraroni Monica , La Vecchia Carlo , Edefonti Valeria
Published: January 2020

Background: Conclusive evidence on foods, nutrients, or dietary patterns and the risk of renal cell carcinoma (RCC) is lacking in the literature. Methods: We considered data from an Italian hospital-based case–control study (1992–2004) on 767 incident RCC cases and 1534 controls. A posteriori dietary patterns were identified by applying principal component factor analysis on 28 nutrients derived from a 78-item food-frequency questionnaire. We estimated the odds ratios (ORs) of RCC and corresponding 95% confidence intervals (CIs) for each quartile category (compared to the lowest one) using conditional multiple logistic regression models providing adjustment for major confounding factors. Results: We identified four dietary patterns, named “Animal products”, “Starch-rich”, “Vitamins and fiber”, and “Cooking oils and dressings”. Higher intakes of the “Starch-rich” pattern were positively associated with RCC risk (OR = 1.38, 95% CI: 1.04–1.82 for the highest quartile, p = 0.018). The association was inverse with the “Cooking oils and dressings” pattern (OR = 0.61, 95% CI: 0.47–0.80, p < 0.001), whereas no association was found with “Animal products” and “Vitamins and fiber” patterns. Conclusions: Higher intakes of starch-related foods may increase RCC risk, whereas consumption of olive and seed oils may favorably influence RCC risk.
Antinociceptive and Anti-Inflammatory Effects of Nypa fruticans Wurmb by Suppressing TRPV1 in the Sciatic Neuropathies
Authors: Kang Mi-sun , Hyun Kyung-Yae
Published: January 2020

Neuropathic pain is generally characterized by sensory abnormalities such as sensory disorders, hyperalgesia, and allodynia. Recent studies have reported that TRPV1 activation is essential for establishing of inflammation in the neuropathy pain models, showing that the expression of this receptor is increased, and contributing to enhanced thermal sensitivity. Nypa fruticans Wurmb (NF), which was used as a folk remedy, is a plant that is gaining attention due to its various effects. In this study, we investigated the antinociceptive and anti-inflammatory effects of NFE (Nypa fruticans Wurmb extracts) by controlling the neurological function of TRPV1. In sciatic crush injury rat models, a significant level of antinociceptive effect was observed in the thermal hyperalgesia test in which NF extracts (NFE 500 mg/kg) were orally administered, daily. Protein quantification of the sciatic nerve and the of the L4–L6 spinal cord showed a decrease of the TRPV1 expression, the inflammatory expression factor, COX2, and proinflammatory factors in the NFE treated groups. Our results indicate that NFE affects antinociceptive and anti-inflammatory by controlling TRPV1 in sciatic neuropathic pain models.
Vitamin K as a Diet Supplement with Impact in Human Health: Current Evidence in Age-Related Diseases
Authors: Simes Dina C. , Viegas Carla S. B. , Araújo Nuna , Marreiros Catarina
Published: January 2020

Vitamin K health benefits have been recently widely shown to extend beyond blood homeostasis and implicated in chronic low-grade inflammatory diseases such as cardiovascular disease, osteoarthritis, dementia, cognitive impairment, mobility disability, and frailty. Novel and more efficient nutritional and therapeutic options are urgently needed to lower the burden and the associated health care costs of these age-related diseases. Naturally occurring vitamin K comprise the phylloquinone (vitamin K1), and a series of menaquinones broadly designated as vitamin K2 that differ in source, absorption rates, tissue distribution, bioavailability, and target activity. Although vitamin K1 and K2 sources are mainly dietary, consumer preference for diet supplements is growing, especially when derived from marine resources. The aim of this review is to update the reader regarding the specific contribution and effect of each K1 and K2 vitamers in human health, identify potential methods for its sustainable and cost-efficient production, and novel natural sources of vitamin K and formulations to improve absorption and bioavailability. This new information will contribute to foster the use of vitamin K as a health-promoting supplement, which meets the increasing consumer demand. Simultaneously, relevant information on the clinical context and direct health consequences of vitamin K deficiency focusing in aging and age-related diseases will be discussed.
Reply to “A Gluten Reduction Is the Patients’ Choice for a Dietary ‘Bottom Up’ Approach in IBS—A Comment on “A 5Ad Dietary Protocol for Functional Bowel Disorders” Nutrients 2019, 11, 1938”
Authors: Ibrahim Fandi , Stribling Philippa
Published: January 2020

We are grateful to Shaw et al [...]
2019: An Awesome Year for Pharmaceuticals
Authors: Vanden Eynde Jean Jacques
Published: January 2020

Ending the year is an opportunity to reflect on the past, in particular the past twelve months [...]
Targeted Polymer-Based Probes for Fluorescence Guided Visualization and Potential Surgery of EGFR-Positive Head-and-Neck Tumors
Authors: Pola Robert , Böhmová Eliška , Filipová Marcela , Pechar Michal , Pankrác Jan , Větvička David , Olejár Tomáš , Kabešová Martina , Poučková Pavla , Šefc Luděk , Zábrodský Michal , Janoušková Olga , Bouček Jan , Etrych Tomáš
Published: January 2020

This report describes the design, synthesis and evaluation of tumor-targeted polymer probes to visualize epidermal growth factor receptor (EGFR)-positive malignant tumors for successful resection via fluorescence guided endoscopic surgery. Fluorescent polymer probes of various molecular weights enabling passive accumulation in tumors via enhanced permeability and retention were prepared and evaluated, showing an optimal molecular weight of 200,000 g/mol for passive tumor targeting. Moreover, poly(N-(2-hydroxypropyl)methacrylamide)-based copolymers labeled with fluorescent dyes were targeted with the EGFR-binding oligopeptide GE-11 (YHWYGYTPQNVI), human EGF or anti-EGFR monoclonal antibody cetuximab were all able to actively target the surface of EGFR-positive tumor cells. Nanoprobes targeted with GE-11 and cetuximab showed the best targeting profile but differed in their tumor accumulation kinetics. Cetuximab increased tumor accumulation after 15 min, whereas GE 11 needed at least 4 h. Interestingly, after 4 h, there were no significant differences in tumor targeting, indicating the potential of oligopeptide targeting for fluorescence-navigated surgery. In conclusion, fluorescent polymer probes targeted by oligopeptide GE-11 or whole antibody are excellent tools for surgical navigation during oncological surgery of head and neck squamous cell carcinoma, due to their relatively simple design, synthesis and cost, as well as optimal pharmacokinetics and accumulation in tumors.
Analgesia Effect of Enteric Sustained-Release Tetrodotoxin Pellets in the Rat
Authors: Hong Bihong , He Jianlin , Sun Jipeng , Le Qingqing , Bai Kaikai , Mou Yanhua , Zhang Yiping , Chen Weizhu , Huang Wenwen
Published: January 2020

Tetrodotoxin (TTX) was identified as a latent neurotoxin that has a significant analgesia effect. It was rapidly absorbed and excreted in rat after intramuscular (i.m.) injection. To maintain the effect, frequent injections were required. The enteric sustained-release TTX pellets with sucrose pellets as a drug carrier was prepared by fluidized bed spray irrigation, coated in sequence with Eudragit NE30D as a sustained-release layer, hydroxypropyl methylcellulose (HPMC) as a barrier layer and Eudragit L30D-55 as an enteric coating. TTX in the pellets could be sustained released for 12 h in dissolution test. In vivo, TTX pellets reached Cmax at 5 h, and t1/2 was 14.52 ± 2.37 h after intragastrically (i.g.) administration in rat. In acetic acid induced writhing test in rat, the pellets at the dosages of 20, 40, 60 and 80 μg·kg−1 produced analgesic effect at about 1.5 h to 9 h and the strongest effect was at about 3 h to 6 h. Simultaneously, the LD50 of the enteric sustained-release TTX pellets was 840.13 μg·kg−1, and the ED50 was about 30 μg·kg−1. Thus, the therapeutic index was about 25. The enteric sustained-release TTX pellets with absolute analgesia effect and greatly enhanced safety was prepared.
Molecularly Imprinted Polymers (MIPs) as Theranostic Systems for Sunitinib Controlled Release and Self-Monitoring in Cancer Therapy
Authors: Parisi Ortensia Ilaria , Ruffo Mariarosa , Malivindi Rocco , Vattimo Anna Francesca , Pezzi Vincenzo , Puoci Francesco
Published: January 2020

Cytotoxic agents that are used conventionally in cancer therapy present limitations that affect their efficacy and safety profile, leading to serious adverse effects. In the aim to overcome these drawbacks, different approaches have been investigated and, among them, theranostics is attracting interest. This new field of medicine combines diagnosis with targeted therapy; therefore, the aim of this study was the preparation and characterization of Molecularly Imprinted Polymers (MIPs) selective for the anticancer drug Sunitinib (SUT) for the development of a novel theranostic system that is able to integrate the drug controlled release ability of MIPs with Rhodamine 6G as a fluorescent marker. MIPs were synthesized by precipitation polymerization and then functionalized with Rhodamine 6G by radical grafting. The obtained polymeric particles were characterized in terms of particles size and distribution, ξ-potential and fluorescent, and hydrophilic properties. Moreover, adsorption isotherms and kinetics and in vitro release properties were also investigated. The obtained binding data confirmed the selective recognition properties of MIP, revealing that SUT adsorption better fitted the Langmuir model, while the adsorption process followed the pseudo-first order kinetic model. Finally, the in vitro release studies highlighted the SUT controlled release behavior of MIP, which was well fitted with the Ritger-Peppas kinetic model. Therefore, the synthesized fluorescent MIP represents a promising material for the development of a theranostic platform for Sunitinib controlled release and self-monitoring in cancer therapy.
Application of Box–Behnken Design to Investigate the Effect of Process Parameters on the Microparticle Production of Ethenzamide through the Rapid Expansion of the Supercritical Solutions Process
Authors: Hsu Yung-Tai , Su Chie-Shaan
Published: January 2020

In this study, the rapid expansion of the supercritical solutions (RESS) process was used to produce microparticles of a commonly used anti-inflammatory drug, ethenzamide. The effects of process parameters in RESS including the extraction temperature, pre-expansion temperature, and post-expansion temperature were investigated using the Box–Behnken design. According to the results of the analysis of variance (ANOVA), the effect of pre-expansion temperature is the most significant parameter on the mean size of RESS-produced ethenzamide. A higher pre-expansion temperature benefits the production of smaller crystals. In addition, a quadratic effect of the post-expansion temperature was also identified. Through RESS, ethenzamide microparticles with a mean size of 1.6 μm were successfully produced. The solid-state properties including the crystal habit, crystal form, thermal behavior, and spectrometric property were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectrometer (FTIR), differential scanning calorimeter (DSC), and powder X-ray diffraction (PXRD). These analytical results show that the rod-like crystals were generated through RESS, and the crystal form, thermal behavior, and spectrometric property of RESS-produced crystals are consistent with the unprocessed ethenzamide.
Multicompartmental Mesoporous Silica/Polymer Nanostructured Hybrids: Design Capabilities by Integrating Linear and Star-Shaped Block Copolymers
Authors: Iatridi Zacharoula , Evangelatou Kyriaki , Theodorakis Nikolaos , Angelopoulou Athina , Avgoustakis Konstantinos , Tsitsilianis Constantinos
Published: January 2020

Poly(2-vinyl pyridine)-b-poly(ethylene oxide) (P2VP-b-PEO) linear diblock copolymer and polystyrene–poly(ethylene oxide) (PS10PEO10) heteroarm star copolymer were used as building elements to prepare organic–inorganic hybrids. By using the layer-by-layer (LbL) methodology, these elements were integrated on mesoporous silica through non-covalent interactions, namely, ionic and H-bonding. For the latter, tannic acid (TA) was used as an intermediate layer. The deposition of the various layers was monitored by thermogravimetric analysis (TGA), electrophoretic measurements, and confocal microscopy. The final silica hybrid, bearing alternating P2VP-b-PEO and PS10PEO10 star layers was capable of carrying one hydrophilic and two hydrophobic chemical species in distinct compartments. These multicompartmental organic–inorganic hybrids could be used as nanostructured carriers for pH-responsive multiple drug delivery and potential theranostic applications.
Adsorptive Removal of Antibiotic Ciprofloxacin from Aqueous Solution Using Protein-Modified Nanosilica
Authors: Pham Tien Duc , Vu Thi Ngan , Nguyen Hai Long , Le Pham Hai Phong , Hoang Thi Sim
Published: January 2020

The present study aims to investigate adsorptive removal of molecular ciprofloxacin using protein-modified nanosilica (ProMNS). Protein was successfully extracted from Moringa seeds while nanosilica was synthesized from rice husk. Fourier-transform infrared (FTIR), ultraviolet visible (UV-Vis) and high-performance liquid chromatography (HPLC) were used to evaluate the characterization of protein. Adsorption of protein onto nanosilica at different pH and ionic strength was thoroughly studied to modify nanosilica surface. The removal efficiency of antibiotic ciprofloxacin (CFX) increased from 56.84% to 89.86% after surface modification with protein. Effective conditions for CFX removal using ProMNS were systematically optimized and found to be pH 7.0, adsorption time 90 min, adsorbent dosage 10 mg/mL, and ionic strength 1 mM KCl. A two-step model was successfully used to fit the adsorption isotherms of CFX onto ProMNS at different ionic strength while a pseudo-second-order model could fit adsorption kinetic of CFX onto ProMNS very well. Maximum adsorption capacity was very high that reached to 85 mg/g. Adsorption of CFX onto ProMNS decreased with increasing KCl concentration, suggesting that adsorption of CFX onto ProMNS is mainly controlled by electrostatic attraction between positively charged ProMNS surface and anionic species of CFX. Adsorption mechanisms of CFX onto ProMNS were discussed in detail based on adsorption isotherms, the change in surface charge by zeta potentail and the change in functional groups by FT-IR. The removal of CFX after three regenerations was greater than 73% while CFX removal from an actual hospital wastewater using ProMNS reached to 70%. Our results suggest that ProMNS is a new and eco-friendly adsorbent to remove antibiotics from aqueous solutions.
Structural Transformation of Polyacrylonitrile (PAN) Fibers during Rapid Thermal Pretreatment in Nitrogen Atmosphere
Authors: Dang Wei , Liu Jie , Wang Xiaoxu , Yan Kaiqi , Zhang Aolin , Yang Jia , Chen Liang , Liang Jieying
Published: January 2020

The modification before the stabilization process could tune the exothermic behavior and the structural evolution of PAN fibers during stabilization. In this study, we demonstrate that a rapid thermal pretreatment in nitrogen can effectively mitigate the exothermic behavior of PAN fibers, such as decreasing the initial temperature, broadening the exothermal peak, and decreasing the nominal heat release during heating the fibers in air. The color of fibers has shown gradual changes from white to light yellow, yellow and brown during thermal pretreatment in nitrogen with the increase of pretreating temperature and time. The differential scanning calorimetry (DSC), Fourier Transform Infrared Spectrometer (FTIR), X-ray diffraction (XRD), and Thermogravimetric Analysis (TG) characterization have been applied to analyze the thermal properties, chemical and physical structural difference between PAN, and thermally pretreated PAN fibers. The thermal pretreatment of PAN fibers in nitrogen could induce cyclization, dehydrogenation, and cross-linking reactions, in which the cyclization play an important role on improving the cyclization index of stabilized PAN fibers. Meanwhile, the pretreatment can result in noticeable changes of the aggregation structure of PAN fibers, as indicated by the increase of crystallinity and crystalline size. These structural modifications can benefit the main cyclization reaction during stabilization and enhance the carbon yield in resultant carbon fibers. The rapid thermal pretreatment in nitrogen could increase efficiency of modification on PAN fibers, and that could save much time and energy. It is beneficial to manufacture low-cost carbon fibers and to spread the applications of carbon fibers.
Lignin-Based Hydrogels: Synthesis and Applications
Authors: Rico-García Diana , Ruiz-Rubio Leire , Pérez-Alvarez Leyre , Hernández-Olmos Saira L. , Guerrero-Ramírez Guillermo L. , Vilas-Vilela José Luis
Published: January 2020

Polymers obtained from biomass are an interesting alternative to petro-based polymers due to their low cost of production, biocompatibility, and biodegradability. This is the case of lignin, which is the second most abundant biopolymer in plants. As a consequence, the exploitation of lignin for the production of new materials with improved properties is currently considered as one of the main challenging issues, especially for the paper industry. Regarding its chemical structure, lignin is a crosslinked polymer that contains many functional hydrophilic and active groups, such as hydroxyls, carbonyls and methoxyls, which provides a great potential to be employed in the synthesis of biodegradable hydrogels, materials that are recognized for their interesting applicability in biomedicine, soil and water treatment, and agriculture, among others. This work describes the main methods for the preparation of lignin-based hydrogels reported in the last years, based on the chemical and/or physical interaction with polymers widely used in hydrogels formulations. Furthermore, herein are also reviewed the current applications of lignin hydrogels as stimuli-responsive materials, flexible supercapacitors, and wearable electronics for biomedical and water remediation applications.
Xiuzhen (Immortality Cultivation) Fantasy: Science, Religion, and the Novels of Magic/Superstition in Contemporary China
Authors: Ni Zhange
Published: January 2020

In early twenty-first-century China, online fantasy is one of the most popular literary genres. This article studies a subgenre of Chinese fantasy named xiuzhen 修真 (immortality cultivation), which draws on Daoist alchemy in particular and Chinese religion and culture in general, especially that which was negatively labelled “superstitious” in the twentieth century, to tell exciting adventure stories. Xiuzhen fantasy is indebted to wuxia xiaoshuo 武俠小說 (martial arts novels), the first emergence of Chinese fantasy in the early twentieth century after the translation of the modern Western discourses of science, religion, and superstition. Although martial arts fiction was suppressed by the modernizing nation-state because it contained the unwanted elements of magic and supernaturalism, its reemergence in the late twentieth century paved the way for the rise of its successor, xiuzhen fantasy. As a type of magical arts fiction, xiuzhen reinvents Daoist alchemy and other “superstitious” practices to build a cultivation world which does not escape but engages with the dazzling reality of digital technology, neoliberal governance, and global capitalism. In this fantastic world, the divide of magic and science breaks down; religion, defined not by faith but embodied practice, serves as the organizing center of society, economy, and politics. Moreover, the subject of martial arts fiction that challenged the sovereignty of the nation-state has evolved into the neoliberal homo economicus and its non-/anti-capitalist alternatives. Reading four exemplary xiuzhen novels, Journeys into the Ephemeral (Piaomiao zhilv 飄渺之旅), The Buddha Belongs to the Dao (Foben shidao 佛本是道), Spirit Roaming (Shenyou 神遊), and Immortality Cultivation 40K (Xiuzhen siwannian 修真四萬年), this article argues that xiuzhen fantasy provides a platform on which the postsocialist generation seek to orient themselves in the labyrinth of contemporary capitalism by rethinking the modernist triad of religion, science, and superstition.
Evaluating Thermal Attribute Mapping Strategies for Oblique Airborne Photogrammetric System AOS-Tx8
Authors: Lin Dong , Bannehr Lutz , Ulrich Christoph , Maas Hans-Gerd
Published: January 2020

Thermal imagery is widely used in various fields of remote sensing. In this study, a novel processing scheme is developed to process the data acquired by the oblique airborne photogrammetric system AOS-Tx8 consisting of four thermal cameras and four RGB cameras with the goal of large-scale area thermal attribute mapping. In order to merge 3D RGB data and 3D thermal data, registration is conducted in four steps: First, thermal and RGB point clouds are generated independently by applying structure from motion (SfM) photogrammetry to both the thermal and RGB imagery. Next, a coarse point cloud registration is performed by the support of georeferencing data (global positioning system, GPS). Subsequently, a fine point cloud registration is conducted by octree-based iterative closest point (ICP). Finally, three different texture mapping strategies are compared. Experimental results showed that the global image pose refinement outperforms the other two strategies at registration accuracy between thermal imagery and RGB point cloud. Potential building thermal leakages in large areas can be fast detected in the generated texture mapping results. Furthermore, a combination of the proposed workflow and the oblique airborne system allows for a detailed thermal analysis of building roofs and facades.
Comparison of Different Multispectral Sensors for Photosynthetic and Non-Photosynthetic Vegetation-Fraction Retrieval
Authors: Ji Cuicui , Li Xiaosong , Wei Huaidong , Li Sike
Published: January 2020

It is very difficult and complex to acquire photosynthetic vegetation (PV) and non-PV (NPV) fractions (fPV and fNPV) using multispectral satellite sensors because estimations of fPV and fNPV are influenced by many factors, such as background-noise interference of pixel-, spatial-, and spectral-scale effects. In this study, comparisons between Sentinel-2A Multispectral Instrument (S2 MSI), Landsat-8 Operational Land Imager (L8 OLI), and GF1 Wide Field View (GF1 WFV) sensors for retrieving sparse photosynthetic and non-photosynthetic vegetation coverage are presented. The analysis employed a linear spectral-mixture model (LSMM) and nonlinear spectral-mixture model (NSMM) to unmix pixels with different spectral and spatial resolution images based on field endmembers; the estimated endmember fractions were later validated with reference to fraction measurements. The results demonstrated that: (1) with higher spatial and spectral resolution, the S2 MSI sensor had a clear advantage for retrieving PV and NPV fractions compared to L8 OLI and GF1 WFV sensors; (2) through incorporating more red edge (RE) and near-infrared (NIR) bands, the accuracy of NPV fraction estimation could be greatly improved; (3) nonlinear spectral mixing effects were not obvious on the 10–30 m spatial scale for desert vegetation; (4) in arid regions, a shadow endmember is a significant factor for sparse vegetation coverage estimated with remote-sensing data. The estimated NPV fractions were especially affected by the shadow effects and could increase root mean square by 50%. The utilized approaches in the study could effectively assess the performance of major multispectral sensors to extract fPV and fNPV through the novel method of spectral-mixture analysis.
Coarse-Resolution Satellite Images Overestimate Urbanization Effects on Vegetation Spring Phenology
Authors: Tian Jiaqi , Zhu Xiaolin , Wu Jin , Shen Miaogen , Chen Jin
Published: January 2020

Numerous investigations of urbanization effects on vegetation spring phenology using satellite images have reached a consensus that vegetation spring phenology in urban areas occurs earlier than in surrounding rural areas. Nevertheless, the magnitude of this rural–urban difference is quite different among these studies, especially for studies over the same areas, which implies large uncertainties. One possible reason is that the satellite images used in these studies have different spatial resolutions from 30 m to 1 km. In this study, we investigated the impact of spatial resolution on the rural–urban difference of vegetation spring phenology using satellite images at different spatial resolutions. To be exact, we first generated a dense 10 m NDVI time series through harmonizing Sentinel-2 and Landsat-8 images by data fusion method, and then resampled the 10 m time series to coarser resolutions from 30 m to 8 km to simulate images at different resolutions. Afterwards, to quantify urbanization effects, vegetation spring phenology at each resolution was extracted by a widely used tool, TIMESAT. Last, we calculated the difference between rural and urban areas using an urban extent map derived from NPP VIIRS nighttime light data. Our results reveal: (1) vegetation spring phenology in urban areas happen earlier than rural areas no matter which spatial resolution from 10 m to 8 km is used, (2) the rural–urban difference in vegetation spring phenology is amplified with spatial resolution, i.e., coarse satellite images overestimate the urbanization effects on vegetation spring phenology, and (3) the underlying reason of this overestimation is that the majority of urban pixels in coarser images have higher diversity in terms of spring phenology dates, which leads to spring phenology detected from coarser NDVI time series earlier than the actual dates. This study indicates that spatial resolution is an important factor that affects the accuracy of the assessment of urbanization effects on vegetation spring phenology. For future studies, we suggest that satellite images with a fine spatial resolution are more appropriate to explore urbanization effects on vegetation spring phenology if vegetation species in urban areas is very diverse.