bluuebunny/crossref_metadata_2025 huggingface dataset:
A genetically encoded red fluorescence dopamine biosensor enables dual imaging of dopamine and norepinephrine
Authors: Nakamoto Chihiro , Goto Yuhei , Tomizawa Yoko , Fukata Yuko , Fukata Masaki , Harpsøe Kasper , Gloriam David E. , Aoki Kazuhiro , Takeuchi Tomonori
Published: May 2020

Dopamine (DA) and norepinephrine (NE) are pivotal neuromodulators that regulate a broad range of brain functions, often in concert. Despite their physiological importance, untangling the relationship between DA and NE in finely controlling output functions is currently challenging, primarily due to a lack of techniques to visualize spatiotemporal dynamics with sufficiently high selectivity. Although genetically encoded fluorescent biosensors have been developed to detect DA, their poor selectivity prevents distinguishing DA from NE. Here, we report the development of a red fluorescent genetically encoded GPCR (G protein-coupled receptor)-activation reporter for DA termed ‘R-GenGAR-DA’. More specifically, a circular permutated red fluorescent protein (cpmApple) was inserted into the third intracellular loop of human DA receptor D1 (DRD1) followed by the screening of mutants within the linkers between DRD1 and cpmApple. We developed two variants: R-GenGAR-DA1.1, which brightened following DA stimulation, and R-GenGAR-DA1.2, which dimmed. R-GenGAR-DA1.2 demonstrated reasonable dynamic range (ΔF/F0= –50%) and DA affinity (EC50= 0.7 µM) as well as the highest selectivity for DA over NE (143-fold) amongst available DA biosensors. Due to its high selectivity, R-GenGAR-DA1.2 allowed dual-color fluorescence live imaging for monitoring DA and NE, combined with the existing green-NE biosensor GRABNE1m, which has high selectivity for NE over DA (>350-fold) in HeLa cells and hippocampal neurons grown from primary culture. By enabling precise measurement of DA, as well as simultaneous visualization of DA and NE, the red-DA biosensor R-GenGAR-DA1.2 is promising in advancing our understanding of the interplay between DA and NE in organizing key brain functions.

Significance Statement

The neuromodulators dopamine and norepinephrine modulate a broad range of brain functions, often in concert. One current challenge is to measure dopamine and norepinephrine dynamics simultaneously with high spatial and temporal resolution. We therefore developed a red-dopamine biosensor that has 143-fold higher selectivity for dopamine over norepinephrine. Taking advantage of its high selectivity for dopamine over norepinephrine, this red-dopamine biosensor allowed dual-color fluorescence live imaging for monitoring dopamine and norepinephrine in both HeLa cells and hippocampal neuronsin vitrocombined with the existing green-norepinephrine biosensor that has 350-fold selectivity for norepinephrine over dopamine. Thus, this approach can provide new opportunities to advance our understanding of high spatial and temporal dynamics of dopamine and norepinephrine in normal and abnormal brain functions.
Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2
Authors: Yao Dehui , Lao Fang , Zhang Zeyi , Liu Yan , Cheng Jianwei , Ding Fengjiao , Wang Xiaofei , Xi Lun , Wang Chuang , Yan Xichong , Zhang Rongkun , Ouyang Fangxing , Ding Hui , Ke Tianyi
Published: May 2020

The outbreak of COVID-19 has so far inflicted millions of people all around the world and will have a long lasting effect on every aspect of everyone’s life. Yet there is no effective approved treatment for the disease. In an effort of utilizing human ferritin as nanoplatform for drug delivery, we engineered a fusion protein by presenting receptor-binding motif (RBM) of SARS-CoV-2 virus spike glycoprotein on the N-terminus of ferritin subunits. The designed fusion protein with a cage-like structure, similar to that of corona virus, is a potential anti-SARS-CoV-2 vaccine. We hereby show the construction, preparation, and characterization of the fusion protein RBM-HFtn. Our initial affinity study confirmed its biological activity towards ACE2 receptor which suggests its mode of action against SARS-CoV-2 could be either through vaccine therapy or blocking the cellular entry of virus as antagonist of ACE2 receptor.
Cell states beyond transcriptomics: integrating structural organization and gene expression in hiPSC-derived cardiomyocytes
Authors: Gerbin Kaytlyn A. , Grancharova Tanya , Donovan-Maiye Rory , Hendershott Melissa C. , Brown Jackson , Dinh Stephanie Q. , Gehring Jamie L. , Hirano Matthew , Johnson Gregory R. , Nath Aditya , Nelson Angelique , Roco Charles M. , Rosenberg Alexander B. , Filip Sluzewski M. , Viana Matheus P. , Yan Calysta , Zaunbrecher Rebecca J. , Cordes Metzler Kimberly R. , Menon Vilas , Palecek Sean P. , Seelig Georg , Gaudreault Nathalie , Knijnenburg Theo , Rafelski Susanne M. , Theriot Julie A. , Gunawardane Ruwanthi N.
Published: May 2020

We present a quantitative co-analysis of RNA abundance and sarcomere organization in single cells and an integrated framework to predict subcellular organization states from gene expression. We used human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes expressing mEGFP-tagged alpha-actinin-2 to develop quantitative image analysis tools for systematic and automated classification of subcellular organization. This captured a wide range of sarcomeric organization states within cell populations that were previously difficult to quantify. We performed RNA FISH targeting genes identified by single cell RNA sequencing to simultaneously assess the relationship between transcript abundance and structural states in single cells. Co-analysis of gene expression and sarcomeric patterns in the same cells revealed biologically meaningful correlations that could be used to predict organizational states. This study establishes a framework for multi-dimensional analysis of single cells to study the relationships between gene expression and subcellular organization and to develop a more nuanced description of cell states.

Graphical Abstract

Transcriptional profiling and structural classification was performed on human induced pluripotent stem cell-derived cardiomyocytes to characterize the relationship between transcript abundance and subcellular organization.
Sirtuin-1 Sensitive Lysine-136 Acetylation Drives Phase Separation and Pathological Aggregation of TDP-43
Authors: Morato Jorge Garcia , Hans Friederike , von Zweydorf Felix , Feederle Regina , Elsässer Simon J. , Skodras Angelos A. , Gloeckner Christian Johannes , Buratti Emanuele , Neumann Manuela , Kahle Philipp J.
Published: May 2020

The trans-activation response DNA-binding protein TDP-43 regulates RNA processing and forms neuropathological aggregates in patients with amyotrophic lateral sclerosis and frontotemporal lobar degeneration. Investigating TDP-43 post-translational modifications, we discovered that K84 acetylation reduced nuclear import whereas K136 acetylation impaired RNA binding and splicing capabilities of TDP-43. Such failure of RNA interaction triggered TDP-43 phase separation mediated by the C-terminal low complexity domain, leading to the formation of insoluble aggregates with pathologically phosphorylated and ubiquitinated TDP-43. Confirming the results from site-directed mutagenesis, we succeeded to introduce authentic acetyl-lysine at the identified sites via amber suppression. [AcK84]TDP-43 showed cytoplasmic mislocalization and the aggregation propensity of [acK136]TDP-43 was confirmed. With newly developed antibodies, we found that the nuclear sirtuin SIRT1 can potently deacetylate [acK136]TDP-43. Moreover, SIRT1 reduced the aggregation propensity of [acK136]TDP-43. Thus, distinct lysine acetylations modulate nuclear import, RNA binding and phase separation of TDP-43, suggesting novel regulatory mechanisms for TDP-43 pathogenesis.
A model of the PI cycle reveals the regulating roles of lipid-binding proteins and pitfalls of using mosaic biological data
Authors: Mazet Francoise , Tindall Marcus J. , Gibbins Jonathan M. , Fry Michael J.
Published: May 2020

The phosphatidylinositol (PI) cycle is central to eukaryotic cell signaling. Its complexity, due to the number of reactions and lipid and inositol phosphate intermediates involved makes it difficult to analyze experimentally. Computational modelling approaches are seen as a way forward to elucidate complex biological regulatory mechanisms when this cannot be achieved solely through experimental approaches. Whilst mathematical modelling is well established in informing biological systems, many models are often informed by data sourced from different cell types (mosaic data), to inform model parameters. For instance, kinetic rate constants are often determined from purified enzyme datain vitroor use experimental concentrations obtained from multiple unrelated cell types. Thus they do not represent any specific cell type nor fully capture cell specific behaviours. In this work, we develop a model of the PI cycle informed byin-vivoomics data taken from a single cell type, namely platelets. Our model recapitulates the known experimental dynamics before and after stimulation with different agonists and demonstrates the importance of lipid- and protein-binding proteins in regulating second messenger outputs. Furthermore, we were able to make a number of predictions regarding the regulation of PI cycle enzymes and the importance of the number of receptors required for successful GPCR signaling. We then consider how pathway behavior differs, when fully informed by data for HeLa cells and show that model predictions remain relatively consistent. However, when informed by mosaic experimental data model predictions greatly vary. Our work illustrates the risks of using mosaic datasets from unrelated cell types which leads to over 75% of outputs not fitting with expected behaviors.

Authors summary

Computational models of cellular complexity offer much in terms of understanding cell behaviors and in informing experimental design, but their usefulness is limited in them being built with mosaic data not representing specific cell types and tested against limited experimental outputs. In this work we demonstrate an approach using quantitative proteomic datasets and temporal experimental data from a single cell type (platelets) to inform kinetic rate constants and protein concentrations for a mathematical model of a key signaling pathway - the phosphatidylinositol (PI) cycle; known for its central role in numerous cell functions and diseases. After using our model to make novel predictions regarding how aspects of the pathway are regulated, we demonstrate its versatile nature by utilising proteomic data from other cell types to generate similar predictions for those cells while highlighting the pitfalls of using mosaic data when constructing computational models.
Structure, function and variants analysis of the androgen-regulatedTMPRSS2, a drug target candidate for COVID-19 infection
Authors: David Alessia , Khanna Tarun , Beykou Melina , Hanna Gordon , Sternberg Michael J E
Published: May 2020

SARS-CoV-2 is a novel virus causing mainly respiratory, but also gastrointestinal symptoms. Elucidating the molecular processes underlying SARS-CoV-2 infection, and how the genetic background of an individual is responsible for the variability in clinical presentation and severity of COVID-19 is essential in understanding this disease.

Cell infection by the SARS-CoV-2 virus requires binding of its Spike (S) protein to the ACE2 cell surface protein and priming of the S by the serine protease TMPRSS2. One may expect that genetic variants leading to a defective TMPRSS2 protein can affect SARS-CoV-2 ability to infect cells. We used a range of bioinformatics methods to estimate the prevalence and pathogenicity of TMPRSS2 genetic variants in the human population, and assess whether TMPRSS2 and ACE2 are co-expressed in the intestine, similarly to what is observed in lungs.

We generated a 3D structural model of the TMPRSS2 extracellular domain using the prediction server Phyre and studied 378 naturally-occurring TMPRSS2 variants reported in the GnomAD database. One common variant, p.V160M (rs12329760), is predicted damaging by both SIFT and PolyPhen2 and has a MAF of 0.25. Valine 160 is a highly conserved residue within the SRCS domain. The SRCS is found in proteins involved in host defence, such as CD5 and CD6, but its role in TMPRSS2 remains unknown. 84 rare variants (53 missense and 31 leading to a prematurely truncated protein, cumulative minor allele frequency (MAF) 7.34×10−4) cause structural destabilization and possibly protein misfolding, and are also predicted damaging by SIFT and PolyPhen2 prediction tools. Moreover, we extracted gene expression data from the human protein atlas and showed that both ACE2 and TMPRSS2 are expressed in the small intestine, duodenum and colon, as well as the kidneys and gallbladder.

The implications of our study are that: i. TMPRSS2 variants, in particular p.V160M with a MAF of 0.25, should be investigated as a possible marker of disease severity and prognosis in COVID-19 and ii. in vitro validation of the co-expression of TMPRSS2 and ACE2 in gastro-intestinal is warranted.
Pharmacokinetics of anti-tuberculosis drugs in multidrug resistant tuberculosis patients in India
Authors: Hemanth Kumar AK , Natarajan PL , Kannan T , Sridhar R , Kumar S , Vinod Kumar V , Gomathi NS , Bharathiraja T , Sudha V , Balaji S , Rameshkumar S , Nair Dina , Tripathy SP , Geetha R
Published: May 2020

Programmatic Management of multidrug resistant tuberculosis (MDR TB) services were introduced in the Indian TB control programme in 2007. A pharmacokinetic (PK) study of drugs used to treat MDR TB, namely levofloxacin (LFX), ethionamide (ETH), cycloserine (CS), pyrazinamide (PZA), moxifloxacin (MFX) and isoniazid (INH) was undertaken in adult MDR TB patients treated according to the prevailing guidelines in India. Factors influencing drug PK and end-of-intensive phase (IP) status were also determined. We recruited 350 MDR TB patients receiving anti-TB treatment (ATT) in the Indian Government programme in south India. At steady state, serial blood samples were collected, after supervised drug administration. Status at end of IP was noted from the programme records. Of the 303 patients for whom end-of-IP status was known, 214 were culture negative (responders), while 45 patients were either culture positive or required change of regimen or had died before completion of IP (non-responders). The median Cmax (2.0 vs 2.9μg/ml; p = 0.005) and AUC0-12 (12.2 vs 17.0μg/ml.h; p = 0.002) of ETH were significantly lower in non-responders than responders at IP. In multivariate logistic regression analysis, after excluding defaulters and adjusting for confounders, AUC0-12 of ETH significantly influenced end-of-IP status (aOR - 1.065; 95% CI: 1.001 - 1.134; p = 0.047). Drug doses used currently in the programme produced optimal drug concentrations in majority of patients. ETH played a major role in the MDR TB combination regimen and was a key determinant of end-of-IP status.
Near, far, wherever you are: simulations on the dose efficiency of holographic and ptychographic coherent imaging
Authors: Du Ming , Gürsoy Dogˇa , Jacobsen Chris
Published: May 2020

Different studies in X-ray microscopy have arrived at conflicting conclusions about the dose efficiency of imaging modes involving the recording of intensity distributions in the near (Fresnel regime) or far (Fraunhofer regime) field downstream of a specimen. A numerical study is presented on the dose efficiency of near-field holography, near-field ptychography and far-field ptychography, where ptychography involves multiple overlapping finite-sized illumination positions. Unlike what has been reported for coherent diffraction imaging, which involves recording a single far-field diffraction pattern, it is found that all three methods offer similar image quality when using the same fluence on the specimen, with far-field ptychography offering slightly better spatial resolution and a lower mean error. These results support the concept that (if the experiment and image reconstruction are done properly) the sample can be near or far; wherever you are, photon fluence on the specimen sets one limit to spatial resolution.
A pedagogical protocol for iterative robotic fabrication on remote grounds
Authors: Bar-Sinai Karen Lee , Shaked Tom , Sprecher Aaron
Published: May 2020

Purpose

The purpose of the paper is to advance remote robotic fabrication through an iterative and pedagogical protocol for shaping architectural grounds. Advancements in autonomous robotic tools enable to reach increasingly larger scales of architectural and landscape construction and operate in remote and inaccessible sites. In parallel, the relation of architecture to its environment is significantly reconsidered, as the building industry's contribution to the environmental stress increases. In response, new practices emerge, addressing the reshaping and modulation of environments using digital tools. The context of extra-terrestrial architecture provides a ground for exploring these issues, as future practice in this domain relies on the use of remote autonomous means for repurposing local matter. As a result, the novelty in robotic construction laboratories is tied to innovation in architectural pedagogy.

Design/methodology/approach

This paper puts forth a pedagogical protocol and iterative framework for digital groundscaping using robotic tools. The framework is demonstrated through an intensive workshop led by the authors. To situate the discussion, digital groundscaping is linked to several conditions that characterize practice and relate to pedagogy. These conditions include the experimental dimension of knowledge in digital fabrication, the convergence of knowledge as part of the blur between the fields of architecture and landscape architecture and the bridging of heterogeneous knowledge sets (virtual and physical), which robotic fabrication on natural terrains entails.

Findings

The outcomes of the workshop indicate that iterative processes can assist in applying autonomous design protocols on remote grounds. The protocols were assessed in light of the roles of technological tools, design iterations and material agency in the robotic fabrication.

Originality/value

The paper concludes with observations linking the iterative protocol to new avenues in architectural pedagogy as means of advancing the capacity to digitally design, modulate and transform natural grounds.
Factors leading to design changes in Jordanian construction projects
Authors: Gharaibeh Lina Ghazi , Matarneh Sandra T. , Arafeh Mazen , Sweis Ghaleb
Published: May 2020

Purpose

The problem of design changes in the construction industry is common worldwide, and the Jordanian market is no exception. The purpose of this paper is to identify the factors causing design changes in construction projects in Jordan in both the public and private sectors. Furthermore, this research will examine the effect of these factors on project's performance during the construction phase.

Design/methodology/approach

This research commences by identifying the factors causing design changes in construction projects worldwide through an intensive literature review. The identified factors were then filtered to those applicable to the Jordanian construction market based on the results obtained from a questionnaire survey and real case construction projects. In total, 252 professionals from the Jordanian construction industry and 10 completed and/or ongoing construction projects in different parts of Jordan were compared.

Findings

The results find that the top major factors affecting design changes are owner's requirements; design errors and omissions and value engineering. The research also studies and documents the impacts of design changes on project cost, schedule and quality.

Originality/value

The results obtained from this research will assist the construction professionals representing owners, consultants and contractors in applying control measures to minimize the occurrence of the identified factors causing design changes and to mitigate their sever impacts on projects in terms of cost, schedule and quality.
Distribution channels of vegetable industry in Indonesia: impact on business performance
Authors: Mariyono Joko , Waskito Jaka , Kuntariningsih Apri , Gunistiyo Gunistiyo , Sumarno Sumarno
Published: May 2020

Purpose

The purpose of this paper is to analyse the distribution channels of vegetable sectors in Indonesia, its economic impact on the performance of vegetable sales and the factors affecting marketing channels selected by producers.

Design/methodology/approach

The study employed qualitative and quantitative methods. A market survey was qualitatively conducted at producer, intermediary, wholesaler, hotel and food processing company as well as retailer levels. Producer survey was quantitatively conducted at the farm level, by interviewing 556 randomly selected farm households. Structural equation modelling was employed to accomplish the objectives of the study.

Findings

Marketing channels for vegetables in Indonesia was complex and relatively long. Farmers decided to select particular channels because of business circumstance and their knowledge. Distance and gentleman’s agreement with traders limited farmers to choose the desirable marketing channel. Marketing channels affect business performance in terms of high sales and profit.

Research limitations/implications

This study only pays attention to the supply side of vegetables. The effect of marketing channels also encumbers the consumers, which are beyond this study. Other studies are expected to highlight the consumer side.

Originality/value

This study focused on smallholder agribusiness players. This study uses two surveys as data sources: market survey and producer survey. The market survey serves as vital information to design producer surveys.
Blending foodscapes and urban touristscapes: international tourism and city marketing in Indian cities
Authors: Amore Alberto , Roy Hiran
Published: May 2020

Purpose

Gateway cities such as Delhi, Mumbai and Kolkata are central in the tourist experience to India, yet the official government authorities and destination marketing organizations tend to underestimate the potential of these destinations to prospective and returning international tourists. In particular, there is little empirical research on urban tourism, food tourism and city marketing in the aforementioned cities. This paper aims to explore the scope for the promotion of Delhi, Mumbai and Kolkata as food urban destinations.

Design/methodology/approach

For the purposes of this study, a case study methodology using content analysis was developed to ascertain the nexus between food and tourism in the three observed cities. Materials were gathered for the year 2019, with a focus on brochures, tourist guides, websites and social media accounts for Delhi, Mumbai and Kolkata. A two-coding approach through NVivo was designed to analyse and report the findings.

Findings

The findings of the study suggest that the cities of Delhi, Mumbai and Kolkata fall short in positioning themselves as food urban destinations. Moreover, the study reports a dissonance between the imagery of Delhi, Mumbai and Kolkata portrayed to international tourists through induced images and the food-related experiences available in the cities. This divide reflects a pattern in destination marketing in India observed in previous research.

Research limitations/implications

The exploratory nature of this study calls for more research in the trends and future directions of food tourism and urban marketing in Indian cities. Moreover, this study calls for further research on the perceptions of urban food experience in Indian cities among international and domestic tourists.

Practical implications

A series of practical implications can be drawn. First, urban and national destination marketing organizations need to join efforts in developing urban marketing campaigns that place food as a key element of the urban experience. Second, cities worldwide are rebranding themselves as food destinations and Indian cities should reconsider local and regional culinary traditions as mean to reposition themselves to food travellers’ similar niche segments.

Social implications

The quest for authenticity is central in the expectations of incoming tourists. Moreover, the richness and variety of local and regional food in the cities analysed in this study can enhance urban visitor experience, with obvious economic and socio-cultural benefits for the local businesses and residents.

Originality/value

This study is the first of its kind to provide preliminary evidence on the nexus between food and tourism in Indian cities. Building from the literature, it developed a conceptual framework for the analysis of food tourism and urban branding and shed light on a currently overlooked aspect of incoming tourism to India.
Exploring Millennial's perceptions towards luxury fashion wearable technology
Authors: Blazquez Marta , Alexander Bethan , Fung Karie
Published: May 2020

Purpose

This study aims to examine the relationship between key value propositions of luxury fashion smartwatches, consumer attitudes and their purchase intentions, and to explore Millennial consumers' overall perceptions of using these wearable technologies.

Design/methodology/approach

The research adopts a mixed methods approach. Quantitative enquiry consisting of 312 respondents was followed by two qualitative focus groups in order to provide a more comprehensive understanding of the issue.

Findings

The findings indicate that functional, individual and social factors influence Millennial consumers' adoption intention of luxury fashion smartwatches. Empirical results reveal that perceived hedonism and usefulness are the most important factors that motivate adoption intentions, followed by subjective norm and perceived conspicuousness, indicating that luxury smartwatches are perceived as both a technological device and luxury fashion accessory.

Originality/value

Given extant research on luxury fashion smartwatches is limited, this study contributes to this unique research stream by exploring Millennial's perceptions towards using these new generation smartwatches. This research develops a theoretical framework building on technology adoption model 2 (Venkatesh and Davis, 2000), theory of reasoned action (Ajzen and Fishbein, 1975) and luxury perception models (Wiedmann et al., 2007).
Foreign aid and growth nexus: empirical evidence from South Asian countries
Authors: Jena Nihar Ranjan , Sethi Narayan
Published: May 2020

Purpose

The purpose of this paper is to empirically examine the effectiveness of foreign aid in improving economic growth prospects in the South Asian region from 1996 to 2017.

Design/methodology/approach

A sample of eight South Asian countries for the period 1996–2017 is being considered for this study. This study uses various econometrics tools such as Pedroni and Johansen–Fisher panel cointegration test, panel fully modified ordinary least square and panel dynamic ordinary least square (PDOLS) to ascertain the long-run and short-run dynamics among the variables under consideration.

Findings

The empirical results found that long-run, as well as the short-run relationship, exist among foreign aid, economic growth, investment, financial deepening, price stability and trade openness of the South Asian economies. The authors also found unidirectional causality running from foreign aid to economic growth. Both the long-run relationship as well as short-run causality between foreign aid and economic growth is unequivocally positive.

Originality/value

This study uses a dynamic macroeconomic modeling framework to assess the impact of aid flows on economic growth in South Asian economies. Taking into account the diversity of level of growth experienced by the eight countries in the Asian region, this study uses an appropriate regression technique, i.e. PDOLS whose results are robust. Therefore, the policymakers in these countries are well-advised to implement suitable policy measures to ensure optimum utilization of foreign capital resources garnered by way of receipt of foreign aid and build on for stronger future economic growth.
Developing an Athlete Brand Identity Scale using Rasch analysis
Authors: Linsner Annika , Hill Brad , Hallmann Kirstin , Sotiriadou Popi
Published: May 2020

Purpose

This study identifies important dimensions of the athlete brand identity construct incorporating the athlete perspective. It also uses Rasch analysis to provide a practical tool (the Athlete Brand Identity Scale) to measure how closely an athlete's personal brand identity is aligned with their perceived brand image.

Design/methodology/approach

Reference to existing athlete branding measurement tools and consultation with ten athlete experts generated (74) items considered important to an athlete brand. Two different response scales were then used to test those items in wider surveys of athletes and consumers. This allowed for further scale development and measurement of congruence between an athlete's self-image and the brand image held by consumers (within the same survey). Factor analysis and Rasch analysis were carried out to refine the item pool and assess item measurement properties to establish a concise scale for determining athlete brand identity.

Findings

Results show successful identification of four dimensions of athlete brand identity measurement: athletic integrity, athletic success, fan engagement and character traits, informed development of the Athlete Brand Identity Scale (ABIdS). The unique and significant aspect of the ABIdS is its capacity to incorporate the athlete's perspective into brand management.

Practical implications

The ABIdS can be utilised by early-career athletes to plan and prioritise branding efforts whilst established athletes can identify incongruence between self-image and consumer perceptions. Such gaps can be evaluated and branding activities modified accordingly. This will enable athletes to better access corporate support/sponsorship thereby reducing reliance on public funds.

Originality/value

The major difference between the ABIdS and other existing scales in the athlete brand research domain is the focus on the athlete perspective, as opposed to the consumer perspectives. Evaluating consumer perspectives does not explain how athletes perceive their own brand or how their own perception of their brand compares to that of people external to the brand (fans and consumers). The ABIdS developed in this study has the potential to achieve this objective as its design was driven by athlete perceptions but tested on both athletes and consumers.
Can foreign aid contribute to sustained growth? A comparison of selected African and Asian countries
Authors: Adams John , Elassal Ola
Published: May 2020

Purpose

Identifying if aid flows have contributed to economic growth or growth divergence between a sample of Asian and African countries is the purpose of this paper. Using data over the period of 1980–2015, the paper attempts to establish whether aid, in any of its forms, has played a role in economic growth in these countries.

Design/methodology/approach

A comprehensive literature analysis over the past 70 years sets the scene for the paper. A panel data fixed-effects model is applied for each sample (Africa and Asia) between 1980 and 2015. Both theoretical predictions and empirical studies are used to derive the independent variables selected for modelling.

Findings

The findings strongly suggest that aid flows in both the Asian and African samples have no relation at all to either long-run growth paths or growth divergence. However, there is a suggestion in the case of the Africa sample that governance decline may well be the primary source of growth divergence.

Research limitations/implications

This result cannot be generalised because it only focuses on six countries but as demonstrated in the paper, other possible samples (from both regions) actually make no difference to the results. It could also be argued (given the comprehensive literature analysis presented here) that it is not essential to have a theoretical relationship between aid and growth because aid is given to different countries with very different characteristics, needs, governance and policy environments.

Practical implications

Donor countries must play a more supervisory role to ensure aid flows are directed to the right channels in recipient countries. Aid should be given to countries which have a certain degree of macroeconomic stability and “good” policy to ensure effectiveness. They also need to pay attention to the sectoral distribution of aid as do recipient countries to better allocate aid flows to productive sectors that contribute to both short- and long-term growth.

Social implications

These are not given much emphasis in this paper.

Originality/value

Most aid–growth studies are based on a large number of countries from different regions with different characteristics or on a single country case. This paper compares between two samples of countries sharing the same characteristics to overcome the heterogeneity problem. This paper is based on a more protracted time series from 1980 to 2015 to capture more accurately the impact of foreign aid on economic growth.
Tax Responses at Low Taxable Incomes: Evidence from Germany*
Authors: Schächtele Simeon
Published: May 2020

The desirability of a particular tax system depends on how different taxpayers react to it. Exploiting the personal allowance threshold and detailed German tax administration data, this paper examines responses at low taxable incomes to extend previous findings. Taxpayers bunch at the allowance threshold, and more so with non‐wage income. Unlike in other studies, wage earners also bunch, at least if they file a tax return, while incomes gross of deductions do not. Deductions account for a sizeable share of the sharp bunching mass of taxpayers with non‐wage income. A machine learning analysis identifies which deduction items predict such sharp bunching. The pattern of results suggests that local intensive‐margin real responses induce moderate deadweight loss.
Effectiveness of probiotics, prebiotics, and prebiotic‐like components in common functional foods
Authors: Peng Mengfei , Tabashsum Zajeba , Anderson Mary , Truong Andy , Houser Ashley K. , Padilla Joselyn , Akmel Ahlam , Bhatti Jacob , Rahaman Shaik O. , Biswas Debabrata
Published: May 2020

The bioactive ingredients in commonly consumed foods include, but are not limited to, prebiotics, prebiotic‐like components, probiotics, and postbiotics. The bioactive ingredients in functional foods have also been associated with beneficial effects on human health. For example, they aid in shaping of gut microflora and promotion of immunity. These functional components also contribute in preventing serious diseases such as cardiovascular malfunction and tumorigenesis. However, the specific mechanisms of these positive influences on human health are still under investigation. In this review, we aim to emphasize the major contents of probiotics, prebiotics, and prebiotic‐like components commonly found in consumable functional foods, and we present an overview of direct and indirect benefits they provide on human health. The major contributors are certain families of metabolites, specifically short‐chain fatty acids and polyunsaturated fatty acids produced by probiotics, and prebiotics, or prebiotic‐like components such as flavonoids, polyphenols, and vitamins that are found in functional foods. These functional ingredients in foods influence the gut microbiota by stimulating the growth of beneficial microbes and the production of beneficial metabolites that, in turn, have direct benefits to the host, while also providing protection from pathogens and maintaining a balanced gut ecosystem. The complex interactions that arise among functional food ingredients, human physiology, the gut microbiota, and their respective metabolic pathways have been found to minimize several factors that contribute to the incidence of chronic disease, such as inflammation oxidative stress.
Preparation and characterization of gellan gum–guar gum blend films incorporated with nisin
Authors: Guo Na , Zhu Guilan , Chen Ding , Wang Dongkun , Zhang Fangyan , Zhang Zhilan
Published: May 2020

Demand for antimicrobial packaging films is growing due to public attention to food safety. The structures and properties of gellan gum–guar gum blend films incorporated with nisin were investigated in this paper. Fourier transform infrared spectroscopy, rheological analyses showed intermolecular interactions among gellan gum, guar gum, and nisin. Furthermore, scanning electron microscopy and thermogravimetric analysis also indicated higher compatibility of the blend film components and better thermal stability than the gellan gum film. Tensile strength (TS), elongation at break (EAB) and water vapor permeability (WVP) of the blend films were enhanced with the addition of guar gum. The TS of the blend film reached 2.89 × 103 MPa, the EAB increased to 67.99%, and the WVP increased to 1.80 × 10−5 g/mm·s·Pa. Additionally, the film with nisin had antibacterial activity for Bacillus subtilis. The results demonstrated that a homogenous and smooth antimicrobial film with gellan gum, guar gum, and nisin could be a good option of antimicrobial packaging film for food preservation.

Practical Application

This work investigated blend package films of gellan gum and guar gum incorporated with nisin. The results showed compatibility and thermal stability of the film were improved with adding a certain amount of guar gum, and also antibacterial activity for Bacillus subtilis of the blend film with nisin. Therefore, it can be used to the development of antimicrobial packaging films.
Determination of biogenic amines in Chub Mackerel from different storage methods
Authors: He Shan , Chen Yaonan , Yang Xianqing , Gao Jingrong , Su Dongxiao , Deng Jianchao , Tian Bin
Published: May 2020

The eight biogenic amines (BA), which are histamine (HIS), tryptamine, putrescine, 2‐phenylethylamine, cadaverine, tyramine, spermidine, and spermine, were determined in Chub Mackerel under different storage conditions after being freshly caught. The storage time and temperature were varied and the guts either present or removed. This study describes a reverse‐phase high‐performance liquid chromatography (HPLC) with a fluorescence detector following precolumn derivation with dansyl chloride method for the determination of BA in in Chub Mackerel samples. HIS represented the highest content of BA regardless of the storage temperature and time. During a 24‐hr period, the content of HIS reached its highest level of 6,466.63 mg/kg at the storage temperature of 30 °C, whereas it only reached 28.73 mg/kg in 24 hr when the storage temperature was 0 °C, which is way below the standard acceptable threshold level (400 mg/kg). The storage times for the content of HIS to exceed the standard threshold level for HIS at different storage temperatures of 0, 4, 10, 15, 20, and 25 °C were 12, 5, 4 days, 48, 36, 15, and 14 hr, respectively. However, these times were significantly shortened when the viscera was removed from freshly‐caught fish before storage. This study not only provides data for monitoring the content of BA, especially HIS, to ensure the safe storage and consumption of freshly‐caught Chub Mackerel, but also presents a cost‐effective method to extend the storage time of freshly‐caught Chub Mackerel.

Practical Application

This study not only provides data for monitoring the content of BA, especially HIS, to ensure the safe storage and consumption of freshly‐caught Chub Mackerel, but also presents a cost‐effective method, which is gut‐removal during storage, to extend the storage time of freshly‐caught Chub Mackerel.
Policy Diffusion: The Issue‐Definition Stage
Authors: Gilardi Fabrizio , Shipan Charles R. , Wüest Bruno
Published: May 2020

We put forward a new approach to studying issue definition within the context of policy diffusion. Most studies of policy diffusion—which is the process by which policymaking in one government affects policymaking in other governments—have focused on policy adoptions. We shift the focus to an important but neglected aspect of this process: the issue‐definition stage. We use topic models to estimate how policies are framed during this stage and how these frames are predicted by prior policy adoptions. Focusing on smoking restriction in U.S. states, our analysis draws upon an original data set of over 52,000 paragraphs from newspapers covering 49 states between 1996 and 2013. We find that frames regarding the policy's concrete implications are predicted by prior adoptions in other states, whereas frames regarding its normative justifications are not. Our approach and findings open the way for a new perspective to studying policy diffusion in many different areas.
Causative role for defective expression of mitochondria‐eating protein in accumulation of mitochondria in thyroid oncocytic cell tumors
Authors: Mussazhanova Zhanna , Shimamura Mika , Kurashige Tomomi , Ito Masahiro , Nakashima Masahiro , Nagayama Yuji
Published: May 2020

Oncocytic cell tumor of the thyroid is composed of large polygonal cells with eosinophilic cytoplasm that is rich in mitochondria. These tumors frequently have the mutations in mitochondrial DNA encoding the mitochondrial electron transport system complex I. However, the mechanism for accumulation of abnormal mitochondria is unknown. A noncanonical mitophagy system has recently been identified, and mitochondria‐eating protein (MIEAP) plays a key role in this system. We therefore hypothesized that accumulation of abnormal mitochondria could be attributed to defective MIEAP expression in these tumors. We first show that MIEAP was expressed in all the conventional thyroid follicular adenomas (FAs)/adenomatous goiters (AGs) but not in oncocytic FAs/AGs; its expression was defective not only in oncocytic thyroid cancers but also in the majority of conventional thyroid cancers. Expression of MIEAP was not correlated with methylation status of the 5′‐UTR of the gene. Our functional analysis showed that exogenously induced MIEAP, but not PARK2, reduced the amounts of abnormal mitochondria, as indicated by decreased reactive oxygen species levels, mitochondrial DNA / nuclear DNA ratios, and cytoplasmic acidification. Therefore, together with previous studies showing that impaired mitochondrial function triggers compensatory mitochondrial biogenesis that causes an increase in the amounts of mitochondria, we conclude that, in oncocytic cell tumors of the thyroid, increased abnormal mitochondria cannot be efficiently eliminated because of a loss of MIEAP expression, ie impaired MIEAP‐mediated noncanonical mitophagy.
Comparison of different morphologies of choroidal neovascularization evaluated by ocular coherence tomography angiography in age‐related macular degeneration
Authors: Tew Teck Boon , Lai Tso‐Ting , Hsieh Yi‐Ting , Ho Tzyy‐Chang , Yang Chung‐May , Yang Chang‐Hao
Published: May 2020

Importance

The clinical implications of different morphologies of choroidal neovascularization (CNV), as evaluated by ocular coherence tomography angiography (OCTA) in neovascular age‐related macular degeneration (nAMD), are lacking.

Background

To describe the morphology of CNV in nAMD using OCTA, and to compare the visual prognosis and other structural OCT biomarkers between different morphologic patterns.

Design

Retrospective cohort study.

Participants

One hundred and forty eyes with nAMD treated with anti‐vascular endothelial growth factor (VEGF).

Methods

Patients were examined using OCTA prior to and at 3, 6 and 12 months after receiving anti‐VEGF therapy.

Main Outcome Measures

Best‐corrected visual acuity (BCVA) and morphologic retinal features.

Results

Organized CNV was identified in 110/140 eyes (78.6%) using OCTA. These CNV complexes could be divided into three OCTA patterns: the ‘medusa’ pattern (n = 41), characterized by branching vessels radiating in all directions; the ‘seafan’ pattern (n = 43), characterized by branching vessels radiating to one side of the lesion; and the ‘tangled’ pattern (n = 26), characterized by globular entwined vessels without a main trunk. At baseline, the eyes with the tangled pattern were from younger patients (P = .031) with better BCVA (P = .007). There were also fewer intraretinal cysts (P = .021), less fibrovascular pigment epithelial detachment (P = .009), and more pachychoroid (P = .007) in eyes with the tangled pattern on OCT. At 12 months post‐treatment, patients with the tangled CNV pattern also showed greater visual improvement than patients with the other two patterns (P = .049).

Conclusions and Relevance

Using OCTA, distinct morphologies of CNV in nAMD patients were identified. These different patterns might be useful predictors for the prognosis of nAMD patients after anti‐VEGF therapy.
Attitudes and knowledge regarding referrals for bariatric surgery among Danish secondary healthcare providers: A national survey
Authors: Elliott Anja F. , B.C. Deepti , Stolberg Charlotte R. , Hepp Nicola , Juhl Anna J. A. , Adhikari Kalyan , Juhl Claus B.
Published: May 2020

Bariatric surgery induces significant and sustained weight loss and subsequently reduces obesity‐related comorbidities. However, only a small percentage of patients with severe obesity undergo bariatric surgery in Denmark. There is limited knowledge about the experiences with and possible reservations to bariatric surgery among secondary healthcare providers. The aim of this cross‐sectional study was to investigate referral patterns and knowledge regarding the criteria for bariatric surgery among Danish secondary healthcare providers, treating obesity‐related diseases. A questionnaire regarding experiences with and reservations to referring patients for consideration for bariatric surgery, along with thoughts to specific patient cases were sent to several specialists: endocrinologists, obstetricians and gynaecologists, orthopaedic surgeons and otorhinolaryngologists. Most questions required responses on a 5‐point Likert scale and frequency distributions were calculated. A total of 345 (44%) specialists responded to the questionnaires. Good knowledge of the criteria for referral to bariatric surgery varied among the specialist from 6% to 68%. One of the main issues was a concern about the medical and surgical postoperative complications, which was a barrier to and influenced referral decisions. Furthermore, specialists were more likely to refer patients to bariatric surgery when patients requested this. Except for endocrinologists, the Danish secondary healthcare specialists interviewed have limited knowledge about bariatric surgery, which results in a reluctance in referring patients. Our results indicate that there is a need to improve knowledge among specialists, regarding the indications, criteria and outcomes for bariatric surgery to establish a more pro‐active, specialist led approach to referrals.