bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Potential of Avenanthramides and their Analogues as α-Glucosidase Inhibitors for Type 2 Diabetes Treatment Utilizing Virtual Screening, Molecular Dynamics, and Drug-likeness Predictions
Authors: Guendouze Assia , Mokrani El Hassen , Benslama Ouided , Lekmine Sabrina
Published: July 2024

Background:

Optimal glycemic control is crucial in type 2 diabetes treatment, with αglucosidase inhibitors emerging as promising candidates. Avenanthramides, compounds found in
oats, exhibit therapeutic potential, but their efficacy as α-glucosidase inhibitors requires thorough
evaluation.

Objective:

This study investigates the potential of avenanthramides and their derivatives as inhibitors
of α-glucosidase for the treatment of type 2 diabetes through comprehensive computational analysis.

Methods:

Structure-Based Virtual Screening of 3543 avenanthramides and their analog compounds
was conducted using FlexX. The top 30 ranked compounds were subjected to visual inspection of
their binding mode within the α-glucosidase binding site to eliminate false positives. The top-ranked
molecule was subjected to dynamic simulation and ADMET prediction.

Results:

The results revealed that 1634 compounds were found to exhibit a greater α-glucosidase
inhibitory potency than miglitol, the reference molecule. Compound S1 exhibited superior αglucosidase inhibitory potency with a binding energy of -45.7786 kJ/mol compared to miglitol,
which had a binding energy of -26.5186 kJ/mol. S1 was predicted to occupy the entire binding site
with an optimized number of hydrogen bonds and hydrophobic interactions. Molecular dynamics
simulations demonstrated that S1 had a lower average RMSD (0.15 ± 0.01 nm) compared to miglitol
(0.16 ± 0.01 nm), indicating superior stability within the α-glucosidase binding site. S1 exhibited
favorable drug-like properties, suggesting its potential as a lead compound for further development
in type 2 diabetes treatment.

Conclusion:

These findings highlight S1's potential for diabetes treatment and pave the way for future experimental investigations. The computational approach utilized offers valuable insights into
the inhibitory potential of avenanthramides, providing a foundation for further drug development for
type 2 diabetes.
Therapeutic Effect of Unmodified Gold-platinum Nanoparticles on Gouty Arthritis Via the Nrf2/HO-1 Pathway
Authors: Liu Zirui , Li Dan , Zhao Baofeng , Mei Xifan
Published: July 2024

Background:

Gouty arthritis, characterized by excruciating pain and discomfort,
poses a significant burden on patients. While nanomedicines have shown promise in addressing
this ailment, their complicated synthesis processes often involve potentially toxic procedures,
contributing to adverse side effects in disease management.

Methods:

In this study, we introduce a straightforward and elegant solution by utilizing easily
prepared gold platinum (AuPt) nanoparticles for the treatment of gouty arthritis. The synthesis of
these nanoparticles involves the use of gold and platinum precursors in conjunction with
NaBH4, simplifying the manufacturing process. Experimental models of gout were established
in both in vivo and in vitro settings through lipopolysaccharide and monosodium urate crystal
induction.

Results:

Our findings revealed that AuPt nanoparticles exhibited potent anti-inflammatory effects
against gout. This effect was attributed to their ability to activate the Nrf2/HO-1 pathway,
resulting in pain alleviation and the inhibition of inflammation, ultimately leading to the reduction
of joint edema. With their uncomplicated synthesis and promising therapeutic potential,
these simply prepared AuPt nanoparticles emerge as a compelling candidate for pharmaceutical
intervention in the treatment of gouty arthritis.

Conclusion:

This approach not only holds the promise of delivering effective relief to patients
but also minimizes the risk of unwanted side effects associated with complex nanomedicine
synthesis processes.
Weaving the Future of Topical Medicine: A Journey with Electrospinning Nanofibre Scaffolds
Authors: Parmar Ghanshyam , Chudasama Jay Mukesh , Aundhia Chintan
Published: July 2024

The pharmaceutical sciences are gradually shielding mankind from a plethora of
deadly but as-of-yet-undiscovered ailments. Many diseases, some of which can be fatal, have
their initial line of defence on the skin. A significant challenge for scientists is the development
of appropriate pharmacological formulations for transdermal drug administration. Low side effect
risk reduces first-pass impact, and good patient compliance makes transdermal distribution
superior to oral delivery. This is why transdermal medication delivery is so important. Thanks to
advancements in pharmaceutical delivery systems, the skin can now absorb medications more
effectively. Researchers from different parts of the globe have investigated many different kinds
of medications as possible transdermal delivery routes using electrospinning nanofibres. The
skin can more easily absorb therapeutic compounds thanks to the nanofibres' ability to concentrate
them. It is possible to load hydrophilic and lipophilic medications onto polymeric nanofibres.
Another option is the transdermal distribution of biopolymer nanofibres. Over long periods
of time, they control the release of medicinal substances. Nanofibres and nanoparticles allow
for the controlled release of both hydrophobic and hydrophilic drugs. Transdermal and topical
medication delivery using polymeric electrospinning nanofibres laden with nanoparticles and
medicines is the subject of this research review. After that, we'll look at some practical instances
of engineers using electrospinning fibres to control the release of drugs in reaction to environmental
and internal factors. Afterwards, we will quickly go over the latest developments in tissue
engineering, hard tissue engineering (which includes repairing musculoskeletal systems,
bones, and cartilage), and cancer therapy that uses electrospin nanofiber scaffolds to control the
distribution of drugs. Thanks to recent advancements in medicine and pharmaceuticals, nanofibres
may soon be able to transport a wide variety of drugs, allowing for more targeted methods
of cellular regeneration and topical medication delivery.
METTL3-mediated m6A Modification of hsa_circ_0131922 Attenuates the Progression of Papillary Thyroid Cancer by Regulating the p53 Pathway
Authors: Li Chan , Xie Ping , Lv Kun , Yang Qian , Mou Yanjie
Published: July 2024

Aims:

This study aimed to confirm the regulatory role and mechanism of circular
RNA (circRNA) hsa_circ_0131922 in Papillary Thyroid Carcinoma (PTC) progression.

Background:

Accumulating evidence suggests that N6-methyladenosine (m6A)-modified circular
RNAs (circRNAs) perform pivotal functions in various malignancies. However, the specific
role of the m6A modification of circRNA mediated by METTL3 in Papillary Thyroid Carcinoma
(PTC) remains undocumented.

Objective:

In this work, we aimed to examine the molecular mechanisms of a novel m6Amodified
circRNA, hsa_circ_0131922, in PTC progression.

Methods:

Potential circRNA was identified from GEO datasets. The RNA or protein levels of
hsa_circ_0131922, METTL3, p53, and p21 were evaluated by qRT-PCR or western blot assays.
The various cellular functions were checked by CCK8, wound healing, transwell, and xenograft
tumor assays. MeRIP-qPCR was performed to observe the METTL3-mediated m6A modification
of hsa_circ_0131922. Furthermore, the interactions between hsa_circ_0131922 and
METTL3 in PTC were analyzed by bioinformatics analysis and various rescue experiments.

Results:

The levels of hsa_circ_0131922 were markedly downregulated in PTC tissues and cell
lines. In addition, the lower hsa_circ_0131922 levels correlated with poor prognosis in PTC
patients. The hsa_circ_0131922 overexpression reduced the malignant phenotypes of PTC cells
and activated the p53/p21 pathway. Bioinformatic analysis showed the m6A-modified sites of
hsa_circ_0131922, and a positive correlation between hsa_circ_0131922 and METTL3. Moreover,
overexpression of METTL3 increased the levels of m6A modification of
hsa_circ_0131922. Mechanistically, the anti-tumor effects of hsa_circ_0131922 overexpression
have been found to be partially reversed by silencing METTL3 in vivo and in vitro.

Conclusion:

The results have demonstrated m6A-modified hsa_circ_0131922 by METTL3 to
attenuate the progression of PTC by regulating the p53 pathway. Therefore, hsa_circ_0131922
could be a predictive prognostic biomarker and therapeutic target for PTC.
Mediating Role of Psychological Safety on Support at the Workplace and its Association with Organization Performance: Context of Indian IT Sector
Authors: Jindal Priya , Mittal Amit , Gupta Piyush , Chaudhry Rishi
Published: July 2024

Introduction

In India, the IT sector has grown to be a substantial industry. Emerging technologies demonstrate their capability to offer clients globally, not only on-shore but also off-shore services. The workplace evolution of the IT sector, which includes a highly structured dynamic system and cutting-edge technology, makes the employees experience anxiety, trepidation, or a general unease while interacting socially or personally with their coworkers. Employees may have anxiety over their capacity to sustain themselves, hold onto their position within the company, and be viewed as valuable contributors by superiors and peers. As the work pressure increases, this helps the organization to understand the impression of psychological safety.

Methods

The current study is an attempt to delve into the association between social support, i.e., Perceived Organizational Support (POS), Perceived Supervisory Support (PSS), and Perceived Co-worker Support (PCS), and personal employee support, i.e., Personal Self-Concept (PSC) at the workplace and Organizational Performance (OP). The study also analyzes the mediating role of Psychological Safety (PS) in this relationship. A cross-sectional survey was conducted to collect data from 232 Indian IT professionals. The data were analyzed using the SPSS statistical software package with Covariance-Based Structural Equation Modeling (CB-SEM). Evaluation of the hypotheses was conducted through the SEM, and mediation analysis was carried out using bootstrap estimation in SPSS AMOS.

Results

The results suggest that POS and PSS have a significant effect on PS and OP. Also, PS has a direct impact on OP. Mediation analysis suggests that PS has full mediation between the relationship of PSC and OP while partial mediation between PSS and OP.

Conclusion

To stay competitive and gain an edge in a continuously changing environment, IT organizations are looking to execute strategies that will help them retain their personnel. Therefore, businesses are keen to provide their employees with psychological safety and a sense of support so that they can perform more effectively in the workplace. Regarding psychological safety, this affects not only the individual but also teams and, thus, the entire company.
Determinants of Small and Rural Local Governments’ Renewable Energy Program Adoption in Cascadia
Authors: Steel Brent Steven , Wolters Erika Allen , Krawchenko Tamara , Farooq Sadaf
Published: July 2024

Aim:

This study aimed to investigate the determinants of renewable energy policy
adoption by small and rural local governments in Cascadia.

Background:

Small and rural local governments currently face many ongoing and numerous new
challenges that complicate their task of sustaining current public services and programs. How
government officials adapt to these changes can affect the long-term viability of local governments in both the U.S. and Canadian contexts.

Objective:

This study has examined the presence or absence of renewable energy programs in
small and rural local governments in the “Cascadia” region of Canada and the U.S. (British Columbia, Oregon, and Washington).

Methods:

Using surveys and interviews of Cascadia local government leaders during the summer
and fall of 2023, correlates of renewable energy policy adoption have been examined, including
cultural, demographic, economic, and political factors.

Results:

Key findings have indicated cities, experiencing population growth, and those with a
progressive political orientation to be more inclined to adopt renewable energy policies. Conversely, remote communities have demonstrated a lower propensity for such adoption. Financial
constraints, evidenced by the impact of inflation and the necessity for service cutbacks, have
been found to negatively correlate with the consideration of renewable energy policies.

Conclusion:

This study has indicated renewable energy projects to be more often found or contemplated in areas being politically liberal, densely populated, and not predominantly rural. It
could be beneficial in shifting the perception of renewable energy from being predominantly an
environmental concern to being recognized for its economic benefits.
De venatione sapientiæ: Nicolás de Cusa y el legado escéptico
Authors: González Fernández Martín
Published: July 2024

The impact of Ambrogio Traversari’s (1386-1439) translation of Diogenes Laertius’ De vitis et sen-tentiæ philosophorum (1433) on Nicholas of Cusa’s De venatione sapientiæ(1462) in relation to the skeptical tradition.
Grammar, Logic, and Cognition: Magnus Hundt (1419-1519) and the Notion of Material Supposition
Authors: Hoenen Maarten J. F. M.
Published: July 2024

In a number of late medieval treatises, the question is raised whether material supposition should be treated in logic or in grammar. Traditionally, this kind of supposition was dealt with in logic. How-ever, some late medieval authors argued that it should rather be treated in grammar. At first glance, this debate may seem to be a fruitless scholastic exercise. Upon closer inspection, however, this issue sheds fundamental light on the different ways in which late medieval scholastics understood the relationship between thoughts, terms, and things.In my paper I will focus mainly on the position of Magnus Hundt, who taught the arts at the Uni-versity of Leipzig towards the end of the fifteenth century. He is not often mentioned in the modern literature. His position, however, is highly remarkable. As will become clear, Hundt’s rejection of material supposition as belonging to the field of logic is illustrative of his strategy of separating logic from grammar, in order to bring it closer to the science of metaphysics
Positive vs. Negative E-WOM: Understanding the More Significant Role in Mediating E-Commerce Quality on Online Impulse Buying
Authors: Arissaputra Rivaldi ,   Universitas Pendidikan Indonesia, Indonesia, Sultan Mokh Adib , Hurriyati Ratih , Gaffar Vanessa , Asmara Maisa Azizah , Sentika Sarah ,   Universitas ‘Aisyiyah Bandung, Indonesia,   Universitas Pendidikan Indonesia, Indonesia,   Universitas Pendidikan Indonesia, Indonesia,   Universitas Pendidikan Indonesia, Indonesia,   Universitas ‘Aisyiyah Bandung, Indonesia,   Universitas ‘Aisyiyah Bandung, Indonesia
Published: July 2024

Electronic Word-of-Mouth (e-WOM) can convey product information about ratings, desires, requests and experiences. Therefore, it plays an important role in influencing purchases. This study aims to identify e-commerce quality, positive e-WOM and negative e-WOM on online impulse buying. E-commerce users sometimes act impulsively in purchasing after seeing some responses contained in e-WOM as information about the products. Positive product responses are not enough to convince e-commerce users to act impulsively, considering negative product responses is also an essential consideration for e-commerce users. This research uses a quantitative approach using a survey method of 315 respondents sampled from the population of e-commerce users in West Java, which was processed using smartPLS. This study found that e-commerce quality directly impacts online impulse buying, positive e-WOM, and negative e-WOM. Positive e-WOM does not directly impact online impulse buying, while negative e-WOM does. Another result shows that negative e-WOM successfully mediates the relationship between e-commerce quality and online impulse buying, while positive e-WOM fails to moderate the relationship between e-commerce quality and online impulse buying. This research contributes to e-commerce and consumer behaviour knowledge, serving as a reference for scholars by exploring how e-commerce quality relates to online impulse buying through positive and negative e-WOM among e-commerce users.
The Effect of Perceived Organizational Support on Intention to Stay Mediated by Employee Engagement and Job Satisfaction
Authors: Pithaloka Dyah Ayu ,   Magister Management Faculty of Economics and Business, Universitas Indonesia, Indonesia, Ardiyanti Niken ,   Magister Management Faculty of Economics and Business, Universitas Indonesia, Indonesia
Published: July 2024

Employees have an important role in a business. Employees are human resources employed by companies who play the role of planners, executors, and drivers in determining the performance and sustainability of the company. Organizational support for employees can be an important factor in retaining employees in the company. The main objective of this study was to determine the perceptions of employee organizational support, employee engagement, and the level of job satisfaction on the employee's intention to stay with the organization. The dataset was generated through a quantitative survey of 233 respondents at PT. Yodya Karya (Persero) from March to April 2023, and analyzed using Structural Equation Modelling (SEM). The findings of this research are that there is no significant and positive direct influence between perceived organizational support on employees' intentions to stay at the company, but rather that perceived organizational support has an impact when there are other things that influence employees' intentions to stay at the company through job satisfaction. Job satisfaction also has a big influence on employees' intentions to stay at the company and employee involvement in the company. Thus, Job Satisfaction at the company needs to be increased to increase employee intentions to stay at the company and also Employee Engagement. Therefore, from the results of this research can be seen that Job Satisfaction becomes an important variable in mediating the influence of Perceived Organizational Support and Intention to Stay.
Fostering Work Readiness in Gen Z through Quintuple Helix, 4P's Creativity
Authors: Daud Ilzar ,   Tanjungpura University, Pontianak, Indonesia, Rahmayani Melia Wida , Nugraha Syahrul , Suryathi Wayan , Hidayat Danny ,   Majalengka University, West Java, Indonesia,   Politeknik Pariwisata Medan, Medan, Indonesia,   Politeknik Negeri Bali, Bali, Indonesia,   Andalas University, Padang, Indonesia
Published: July 2024

This study aims to explore gender differences in creativity exploration and work readiness among Generation Z employees in the workplace. It integrates the Quintuple Helix model with the 4P's creativity model to comprehensively understand how gender dynamics influence individuals' creative potential and readiness to thrive professionally. The study employed a quantitative approach, distributing questionnaires to 501 students with entrepreneurial experience. Path analysis with Partial Least Square (PLS) was used to analyze the data, including factors like factor loading, construct reliability, Average Variance Extracted (AVE), and discriminant validity. Structural equation modelling assessed direct, indirect, and overall effects. The results indicate that integrating the Quintuple Helix and 4P's creativity models positively impacts creativity exploration and work readiness. Gender dynamics influence individuals' self-perceptions, participation in creative processes, creative outcomes, and access to opportunities. Addressing gender biases and promoting inclusivity in skill development, collaborative processes, product design, leadership, and supportive policies can enhance work readiness for individuals of all genders. The study suggests that organizations and policymakers should proactively challenge gender stereotypes and biases, foster an inclusive culture, provide equal skill development opportunities, promote collaboration, challenge product biases, encourage gender diversity in leadership, and implement supportive policies. These actions can create a more equitable and innovative work environment that harnesses the full creative potential of the workforce and enhances overall work readiness.
READING CORNER AS A MEANS OF INSTILLING CHARACTER VALUES AT AL-FALAH BOARDING SCHOOL
Authors: Noveria Anggraeni Fiaji  , Muhammad Hambali  , Trisna Andarwulan  , Prima Zulvarina
Published: July 2024

Al Falah Islamic Boarding School was founded in 1989 as one of the first Muhammadiyah Islamic Boarding Schools in Gorontalo consisting of Madrasah Tsanawiyah and Madrasah Aliyah. Based on data on daily activities carried out at the Al Falah Islamic Boarding School, it is still not visible that there are maximum literacy activities held every day, of course this has more or less had an impact on the scientific level of Al Falah students because literacy is one of the basic foundations of an educational activity. Good literacy activities can have an impact on good academic achievement and awareness of education. Literacy activities must of course implement a good system in all aspects of the activity, not least of which must be supported by adequate facilities. However, facilities in the form of infrastructure, especially reading books or other educational supporting books, are not yet fully available in the Al Falah library. This should be used as one of the Islamic boarding school's superior programs and as a culture that must be continuously developed and renewed. As a solution, community service was carried out using socialization method to the ustadz and ustadzah caretakers of the Al-Falah Islamic Boarding School to create a reading corner program as a means of literacy for students at Al Falah Islamic Boarding School. Not only reading corners, other literacy methods have also been designed in this community service program.
KESETARAAN GENDER PADA PEMBAGIAN TUGAS ABDI DALEM PUNAKAWAN TEPAS DI KERATON YOGYAKARTA
Authors: Ahmad Zia Khusnullabib , Hanum Farida
Published: July 2024

Keraton Yogyakarta berfungsi sebagai pusat pemerintahan kesultanan Yogyakarta yang masih bertahan hingga kini. Di dalam lingkungan Keraton Yogyakarta aktivitas Sultan di bantu oleh abdi dalem. Pembagian-pembagian tugas diberikan kepada tiap-tiap abdi dalem. Tujuan penelitian ini adalah untuk mengetahui kesetaraan gender dan mendeskripsikan pembagian tugas abdi dalem punakwan tepas laki-laki dan perempuan di Keraton Yogyakarta. Metode penelitian yang digunakan adalah deskriptif kualitatif. Populasi dalam penelitian ini adalah abdi dalem punokawan tepas baik laki-laki maupun perempuan dengan teknik sampling purposive sampling. Teknik pengumpulan data yang digunakan adalah observasi, wawancara, dan dokumentasi. Teknik analisis yang digunakan yaitu teknik analisis analisis gender yaitu teknik analisis Harvard. Hasil penelitian ini menyatakan bahwab aktivitas abdi dalem laki-laki dan perempuan terdapat pembagian kerja yang sama, walau beberapa aktivitas yang hanya boleh dilakukan laki-laki saja dan yang hanya boleh dilakukan perempuan saja, hal ini dikarenakan masih mengadopsi tradisi yang telah lama dilakukan. Hak yang diperoleh abdi dalem laki-laki dan perempuan relatif sama, pada umumnya para abdi dalem bekerja karenarasa pengabdian untuk keraton dan melestarikan budaya jawa. Dampak yang diperoleh abdi dalem laki-laki dan perempuan sama, yaitu senang dan memiliki rasa kebanggan dan ketenangan dalam bekerja sebagai abdi dalem punakawan tepas.
POTENSI TAMAN KULINER WONOSARI DALAM MENDUKUNG PARIWISATA DI KABUPATEN GUNUNGKIDUL
Authors: Ramadhani Vidya Putri , Pinasti V. Indah Sri
Published: July 2024

Penelitian ini mendeskripsikan mengenai potensi Taman Kuliner Wonosari dalam mendukung pariwisata di Kabupaten Gunungkidul serta upaya pihak pengelola untuk menarik para pengunjung dan mengoptimalkan fungsi dari Taman Kuliner Wonosari sebagai tujuan wisata. Lokasi penelitian berada di Taman Kuliner Wonosari. Penelitian ini menggunakan metode kualitatif deskriptif. Pengambilan sampel dengan menggunakan teknik purposive sampling dengan jumlah informan sebanyak 12 orang. Teknik pengumpulan data dilakukan dengan observasi, wawancara, dan dokumentasi. Validitas data yang digunakan adalah triangulasi sumber. Data yang diperoleh kemudian dianalisis secara kualitatif menggunakan metode interaktif Miles dan Huberman meliputi pengumpulan data, reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa Taman Kuliner Wonosari memiliki berbagai potensi yang dapat dikembangkan sebagai tujuan wisata khususnya wisata kuliner di Kabupaten Gunungkidul. Namun terdapat beberapa kendala yang sampai saat ini masih diupayakan solusinya oleh pihak pengelola. Pemerintah Kabupaten Gunungkidul bersama dengan pihak-pihak pengelola Taman Kuliner Wonosari terus berupaya melakukan perbaikan dan pengembangan Taman Kuliner Wonosari sehingga benar-benar layak menjadi destinasi wisata di Kabupaten Gunungkidul.
ANALISIS STUDENT COURSE ENGANGEMENT DAN FAKTOR-FAKTOR YANG MEMPENGARUHINYA: STUDI KASUS PADA MAHASISWA FAKULTAS EKONOMI UNIVERSITAS NEGERI MEDAN
Authors: Nurwendari Weny , Silalahi Sondang Aida , Harefa Kornelius , Nurhayani Ulfa
Published: July 2024

Sebagai mahasiswa, mereka memiliki tanggung jawab untuk belajar dan memenuhi semua kewajiban akademik yang ada. Namun, di sisi lain, mereka juga diharapkan untuk terlibat secara aktif dalam kegiatan yang terkait dengan perkuliahannya. Dalam penelitian ini, digunakan konsep keterlibatan mahasiswa secara umum dalam konteks perkuliahan untuk memudahkan pengumpulan data utama dan pendukung. Tujuan dari penelitian ini adalah untuk mengevaluasi sejauh mana atau seberapa besar mahasiswa terlibat dalam proses perkuliahan di Fakultas Ekonomi Universitas Negeri Medan. Selain itu, tujuan dari penelitian ini juga melibatkan pengujian hubungan antara keterlibatan mahasiswa dalam perkuliahan dengan variabel lainnya, seperti Indeks Prestasi Kumulatif (IPK), jenis kelamin, dan jumlah kegiatan yang diikuti baik di dalam dan di luar kampus. Studi ini dilakukan dengan metode survei pada mahasiswa yang aktif megikuti kegiatan perkuliahan yaitu mahasiswa yang sudah mengisi KRS pada semester genap. Data yang terkumpul akan di uji dengan structural equational modeling (SEM). Hasil dari penelitian ini menunjukkan bahwa mahasiswa pendidikan akuntansi memiliki student course engagement yang tinggi serta varibel IPK memiliki pengaruh terhadap student course engagement dengan t-statistik 1.965, Jenis kelamiin berpengaruh terhadap student course engagement dengan t-statistik 15.166, kegiatan luar dan dalam kampus berpengaruh terhadap student course engagement dengan t-statistik 10.958.
Nutritional Profile and Topic Management for Wound Healing in Children with Epidermolysis Bullosa: What Is the Evidence? A Systematic Review
Authors: dos Santos Maria Lúcia , Monteiro Ana Cristina , Nascimento Andréa , Barbosa Sívia , Delgado Artur , Zamberlan Patrícia
Published: July 2024

Epidermolysis Bullosa (EB) is a rare genetic disorder characterized by fragile skin that blisters and tears easily, leading to significant morbidity and mortality. Depending on the specific genetic mutations and the proteins involved, EB can be classified into several subtypes whose molecular complexity is compounded by the variability in mutation types (missense, nonsense, insertions, deletions), their locations within the genes, and the resultant effects on protein function. This systematic review aimed to identify and synthesize available evidence on wound healing interventions and the nutritional profile of children diagnosed with EB. A comprehensive search yielded 28 articles, including 21 clinical trials and seven observational studies, encompassing 994 patients with various EB subtypes. The majority of studies described subtypes such as Simplex EB (EBS), Junctional EB (JEB), Dystrophic EB (DEB), and EB Kindler. The primary interventions for wound healing included dressings with collagen, biocellulose, and various topical creams. Nutritional assessment was limited, with only six studies examining nutritional status, predominantly through anthropometry and dietary intake analysis. Subgroup analyses indicated higher malnutrition rates among patients with DEB compared to JEB. The review underscores the importance of addressing wound healing and nutritional challenges in EB management. Further research is needed to explore effective interventions and optimize care for this vulnerable population.KeywordsNutrition; pediatrics; wound healing; epidermolysis bullosa
Acupuncture as Adjuvant Therapy for Glaucoma: Protocol for a Randomized Controlled Trial
Authors: Liao Yi-Fang , Lee Yu-Chen , Lin Hui-Ju , Shao Yi-Ching
Published: July 2024

Background

Glaucoma is a chronic progressive optic neuropathy that necessitates lifelong treatment to reduce the decline of the optic nerve. Due to the extended and continuous treatments required for patients, complementary therapies are often considered alongside conventional treatments to enhance the effectiveness of the treatment. Acupuncture has demonstrated the potential to lower intraocular pressure in previous clinical trials, making it a promising glaucoma intervention.

Objective

The primary objective of this study is to conduct a single-center randomized control trial involving patients with glaucoma. Acupuncture will be evaluated as an adjunctive therapy. The trial aims to explore its effectiveness for glaucoma.

Methods

In this single-center randomized controlled trial, participants (N=50) with primary open-angle glaucoma will be randomly assigned to the treatment group, receiving ophthalmic acupuncture with “De Qi” sensation, or the control group, receiving minimum acupuncture stimulation on nonophthalmic acupoints. The intervention will consist of weekly acupuncture treatments for a total of 6 sessions. Participants will be assessed at 8 time points, which are baseline, during the intervention (6 times), and at a 3-month follow-up. The primary outcome measure is a change in the intraocular pressure before and after each acupuncture treatment. Secondary outcomes will include measurements of heart rate and blood pressure before and after acupuncture, best-corrected visual acuity, visual field, optical coherence tomography, optical coherence tomography angiography, the Glaucoma Symptom Scale, and the Glaucoma Quality of Life-15 questionnaire.

Results

Recruitment of participants for the trial commenced on June 28, 2023. A total of 10 participants have been enrolled to test the feasibility of the experiment. We anticipate that the preliminary data from this trial will be completed by December 2025.

Conclusions

This trial uses rigorous methodology and comprehensive outcome measurements to assess the clinical efficacy of acupuncture as an adjunctive therapy for glaucoma, providing valuable insights for future clinical treatment guidelines.

Trial Registration

ClinicalTrials.gov NCT05753137; https://clinicaltrials.gov/study/NCT05753137

International Registered Report Identifier (IRRID)

DERR1-10.2196/57888
Quantification of Urinary Exosomal Prostate-Specific Antigen for the Diagnosis of Prostate Cancer Using Clinical Laboratory–Based Techniques: Protocol for a Case-Control Study
Authors: Li Guorong , Tholance Yannick , Mallouk Nora , Waeckel Louis , Flandrin Pascale , Bali Bruno , Badet Lionel , Cornillon Pierre
Published: July 2024

Background

Prostate cancer is the most common cancer in men and represents a major public health problem. The current method for the diagnosis or screening of prostate cancer is invasive and costly. There have been renewed and innovative studies searching for urinary biomarkers to aid in the diagnosis of prostate cancer, especially with technologies based on urinary exosomes. However, technologies based on urine exosomes usually need expensive machines such as an ultracentrifuge and they are difficult to standardize, which hinder their application in clinical laboratories. We have optimized and standardized the isolation of urinary exosomes with the precipitation method. We have found that urinary exosomal prostate-specific antigen (PSA) can be quantified by automatic Elecsys total PSA technique.

Objective

In this study, our objective is to utilize urinary exosomes from prostate cancer for the development of a test to aid in its diagnosis.

Methods

Exosomes from the prostate cancer cell line LNCaP was used to set up the technique. To analyze urine samples from patients, the methods include the collection of first-void urine using the Colli-Pee device, the isolation of urine exosomes using the optimized precipitation method, and the quantification of exosomal PSA by Elecsys total PSA.

Results

This will be a 2-year study. We will start including patients and controls in the last quarter of 2024. We expect the results to be published in the second quarter of 2027.

Conclusions

This is the first study to quantify urinary exosomal PSA using the Elecsys total PSA technique for the diagnosis of prostate cancer. This study emphasizes techniques that are suitable for implementation in clinical laboratories, which will facilitate the application of urinary exosomes to simplify and improve the diagnosis and screening of prostate cancer.

International Registered Report Identifier (IRRID)

PRR1-10.2196/63551
Authors’ Response to Peer Reviews of “Establishment of a Novel Fetal Ovine Heart Cell Line by Spontaneous Cell Fusion: Experimental Study” (Preprint)
Authors: Suleiman Khalid , Aljulidan Mutaib , Hussein Gamaleldin , Alkhalaf Habib
Published: July 2024

UNSTRUCTURED
Peer Review of “Establishment of a Novel Fetal Ovine Heart Cell Line by Spontaneous Cell Fusion: Experimental Study” (Preprint)
Authors: Rafi Hira
Published: July 2024

UNSTRUCTURED
The prerequisites for artificial intelligence in Danish general practice: A qualitative vignette study among general practitioners (Preprint)
Authors: Jørgensen Natasha Lee , Merrild Camilla Hoffmann , Jensen Martin Bach , Moeslund Thomas B. , Kidholm Kristian , Thomsen Janus Laust
Published: July 2024

BACKGROUND

Artificial intelligence has been deemed revolutionary in medicine, but very few artificial intelligence solutions have been observed in Danish general practice. General practice in Denmark has an excellent system of digitization to develop and utilize artificial intelligence. However, a lack of involvement of general practitioners in the development of artificial intelligence exists. The perspectives of general practitioners as end users are essential to facilitate the development and implementation of artificial intelligence in general practice.

OBJECTIVE

This study aimed to characterize the prerequisites that must be met to enable the development and implementation of artificial intelligence in Danish general practice.

METHODS

This study applied semi-structured interviews and vignettes to gain perspectives on the potential for developing and implementing artificial intelligence among general practitioners. Twelve general practitioners were interviewed, resulting in an exhaustive dataset. The interviews were transcribed, and thematic analysis was conducted to identify the dominant themes throughout the data.

RESULTS

Four main themes were identified in the data analysis as prerequisites that general practitioners found important to consider when developing and implementing AI in general practice: ‘AI must begin with the low-hanging fruit’, ‘AI must be meaningful in the GP’s work’, ‘The GP-patient relationship must be maintained despite AI’, and ‘AI must be a free, active, and integrated option in the EHR’.

CONCLUSIONS

The four themes contributing to defining prerequisites for artificial intelligence can potentially lead the first steps of future development and implementation of artificial intelligence in Danish general practice. The participating general practitioners were positive towards developing and implementing artificial intelligence in their clinics, and it would be valuable to consider the defined prerequisites when considering new artificial intelligence tools for general practice.
bfGPS®: A mobile app for promoting breastfeeding-friendly communities. A design and development study (Preprint)
Authors: Fan Heidi Sze Lok , Leung Emily Tsz Yan , Lau Ka Wing , Wong Janet Yuen Ha , Choi Edmond Pui Hang , LAM Christine , Tarrant Marie , Ngan Hextan Yuen Sheung , Ip Patrick , Lin Chia Chin , Lok Kris Yuet Wan
Published: July 2024

BACKGROUND

Breastfeeding is vital for the health and well-being of both mothers and infants, and it is crucial to create supportive environments that promote and sustain breastfeeding practices.

OBJECTIVE

This study aims to describe the process followed the development of an application called “bfGPS” to provide territory-wide information on breastfeeding facilities, with the goal of fostering a breastfeeding-friendly community. We also gain insights into how breastfeeding individuals and community perceive and experience bfGPS.

METHODS

For the design, evaluation and development of the app, two qualitative studies were conducted, applying a user-centered design approach. For the performance testing, ten focus groups and 19 one-on-one interviews were conducted with staff working in public premises (n=29) and breastfeeding individuals (n=29). For the usability evaluation, semi-structured, in-depth, one-to-one interviews were conducted with breastfeeding individuals (N=30). Content analysis was used to analyze the data.

RESULTS

The development of the bfGPS was divided into three phases: (1) planning, prototyping and performance testing, (2) implementation and updates, and (3) usability evaluation. Three main themes emerged from the usability evaluation: bfGPS improves the community experience for breastfeeding individuals, facilitates tracking the infant’s growth, and provides suggestions for further development.

CONCLUSIONS

bfGPS application is the first user-friendly tool designed to assist users in locating breastfeeding facilities within the community. It stands as a guide for similar healthcare app developments, emphasizing the importance of accurate, current data to ensure user adoption and sustained use. The app’s potential lies in the support and reinforcement of breastfeeding practices, couple with self-management strategies.
A Digital Archaeology of Early Hispanic Film Culture: Film Magazines and the Male Fan Reader
Authors: Torres-Cacoullos Anna , Senatorova Elizaveta
Published: July 2024

As a sociologically-oriented study, this project contributes to an archaeology of cinema fandom broadly, and early Spanish fan culture specifically, by spotlighting male readers of popular film magazines. Taking as an exploratory case study reader interactivity with the magazine Popular Film, analysis of correspondence and published photos of readers participating in reader contests demonstrates that the magazine’s cinema fan base was composed of a strikingly large proportion of readers who were male and that these were ardent enthusiasts of celebrity consumer culture. This is a notable contradistinction to the widely-held idea of the star-struck female movie fan. Methodologically, in conducting this study we reflect on the challenges of digital approaches to historical periodical research, where particular challenges are posed when working with magazines in a non-anglophone language, and when there are few baseline studies to rely on to guide and contextualize patterns picked up through strictly macro methods. We advocate for the affordances of a mixed macro-micro approach that combines distant reading with traditional textual studies of close reading. By adopting such a hybrid framework, digital methods provide new opportunities towards reconstructing profiles of magazine readerships and to unearth evidence of male movie fans in Spain.
Soviet View of the World. Exploring Long-Term Visual Patterns in “Novosti dnia” Newsreel Journal (1945-1992)
Authors: Oiva Mila , Ohm Tillmann , Mukhina Ksenia , Canet Solà Mar , Schich Maximilian
Published: July 2024

Newsreels, short documentary news films, were an influential channel of mass communication and propaganda in the Soviet Union. They served as an important means of visualizing the world for audiences in the way the Soviet authorities wanted it to be depicted. Studies in Soviet visual culture have recognized both continuities of repeating patterns and changes in the post-World War II period. This understanding is based primarily on temporally limited source selections, while a more systematic study of the developments in Soviet visual culture over a longer period is pending. In this article, we reveal long-term continuities, subtle changes, and sudden shifts in the official visual discourse in the Soviet newsreel series ‘Novosti dnia’ (News of the Day) 1945 to 1992. We study visual patterns in approximately 1,700 digitized newsreel issues, each about ten minutes long, using multidimensional vector embeddings. These embeddings, produced from the central frames of 205,678 shots, help visually evaluate the footage and assess visual similarities based on ResNet50 feature vectors. For this, we use the Collection Space Navigator tool. The article demonstrates how multidimensional vector embeddings can be used to study the internal time of the films, and the external time of the years running by.
Об организации технического обслуживания и ремонта станочного парка предприятия в формате «по техническому состоянию»
Authors: Толстых Л.П. , Гора С.М. , Медведев Н.К. , Медведев В.К.
Published: July 2024

Предложена методология перехода от технического обслуживания и ремонта «по регламенту» к техническому обслуживанию и ремонту «по техническому состоянию» (ТОиР-ТС). Рассмотрена разработка комплекса национальных стандартов, относящихся к функционированию системы ТОиР-ТС, для создания нормативно-­технической базы перехода.
A note on P-Sasakian manifolds satisfying certain conditions
Authors: Uygun Pakize , Atçeken Mehmet
Published: July 2024

In the present paper, we have studied the curvature tensors of PSasakian manifold. For a P-Sasakian manifold, W1 ·S = 0, W1 ·Z = 0 and W9 · W1 = 0 cases are considered. According these cases, PSasakian manifolds have been characterized such as η-Einstein and Einstein. In addition, we research W1-flat and W9-flat for a PSasakian manifold. The results are interesting and give an idea about the geometry of P-Sasakian manifold.
Identification of Betulinic Acid and Trimethoxyellagic Acid as the Antidiabetic Compounds in Anogeissus leiocarpus Stem Bark Purified Extract
Authors: Esievo King Akpofure Nelson , Balogun Emmanuel Oluwadare , Esievo Kingsley Oghenerukevwe , Esievo Lauretta Oghenekevwe , Esievo Edith Monica , Sani Dahiru , Wassagwa John , Uyovbisere Edward Oniovosa , Mumah Emmanuel Tamajong
Published: July 2024

Objectives: The study aimed to identify the antidiabetic compounds purified from stem bark of Anogeissus leiocarpus and propose the mechanisms of action.
Design: Anogeissus leiocarpus stem bark was purified through ethyl acetate and n-hexane with minor exceptions. For very clear separation, cold acetone was added to trigger the precipitation. The precipitate was dissolved with a mixture of DCM:methanol (9:1), adsorbed it to silica gel (5 g), evaporated to free flowing powder and fractionated it over silica gel (50 g) to realize 40 fractions. The gummy fractions were ignored. The light brown powder which possessed antidiabetic effect was selected for Nuclear Magnetic Resonance for structural elucidation.    
Nuclear Magnetic Resonance (NMR) and Determination of Structure of Purified Compound: After column chromatography and TLC processes, along with the cold acetone, to the point of crystallization, the purified compounds, the light brown powder, were presented to NMR (Bruker Avance III, Spectrometer frequency 400 MHz; solvents DMSO-do, CDCl3, Acetone-d6. Institute of Chemistry, Strathclyde University Glasgow UK.) for determination of compound structures and analyses.
Results: The NMR spectra and analyses revealed the existence of Betulinic acid and Trimethoxyellagic acid.
Conclusion: We show that Betulinic acid and Trimethoxyellagic acid are potent antidiabetic compounds in the stem bark extract of A. leiocarpus.
Keywords: Anogeissus leiocarpus, Betulinic Acid, Trimethoxyellagic Acid, Antidiabetic agent
The MeViDa Study: Bioequivalence Study of FDC of Dapagliflozin, Vildagliptin SR and Metformin SR in Healthy Indian Volunteers: A Randomized, Open-Label, Crossover Study
Authors: Pednekar Abhijit , Gupta Amit , Warrier Sona
Published: July 2024

Objective: To assess the pharmacokinetics and bioequivalence of Dapagliflozin 10mg + Vildagliptin Sustained Release (SR) 100mg + Metformin SR 1000mg fixed dose combination (FDC) tablets with DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin SR 1000 mg tablets) in healthy adult male subjects under fasting conditions.
Material and Methods: This was an open-label, balanced, randomized, two-treatment, two-period, two-sequence, single-dose, crossover, oral bioequivalence study. Volunteers were randomized to receive either a test product or a reference product under the fasting condition with a seven-day washout period. The pharmacokinetics parameters evaluated were maximum plasma concentration (Cmax), the area under the curve (AUC) at time t (AUC0-t), and the total area under the curve (AUC0-∞). Adverse events were also assessed as safety endpoints.
Results: Twenty-four healthy adult male subjects were randomized, and 24 completed the study. The mean values for Cmax, AUC0-t, and AUC0-∞ were almost identical for test and reference products. The 90% confidence intervals of the ratios of adjusted geometric means for the pharmacokinetic parameters, i.e. Cmax, AUC0-t, and AUC0-∞ of the test product, were within the predefined bioequivalence limits of 80.00 to 125.00%. No adverse events or serious adverse events or deaths were reported during the study. All treatments were well tolerated.
Conclusion: The test formulation, Dapagliflozin 10mg + Vildagliptin SR 100mg + Metformin SR 1000mg FDC, were bioequivalent to DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin 1000mg SR tablets) in healthy adult male subjects under fasting conditions.
Keywords: Bioequivalence, Fixed-Dose Combination, Dapagliflozin, Metformin, Sustained Release, Vildagliptin, AUC, Cmax
Method development and validation for estimation of Gliclazide in bulk and tablet form by UV Spectrophotometer
Authors: Hanwate Ravindra , Singh Bhupendra , Khairnar Nilesh , Chavan Sushmita
Published: July 2024

UV Spectrophotometric method has been developed to determine Gliclazide in bulk pure and tablet forms. It is a simple, accurate, reproducible, rapid and less time-consuming method. The maximum wavelength of the drug was found to be 232nm. Beer Lamberts’ law was obeyed in the concentration range of 2-20 µg/ml.  (LOD)The limit of detection and limit of qualification (LOQ) was found to be 0.16 µg/ml and 0.50 µg/ml from this method per cent recovery of the drug was found to be 99.30% which indicates no interaction of the excipients. This method was found accurate, simple, precise and rapid for determination of tablet dosage form.
Keywords: validation, Gliclazide, tablets, UV Spectroscopy, LOD, LOQ