bluuebunny/crossref_metadata_2025 huggingface dataset:
Missense Mutations in the CITED2 Gene Contribute to Congenital Heart Disease
Authors: Yaqoob Hira , Ahmad Hussain
Published: March 2024

Background
Congenital heart disease (CHD) is a lifelong abnormality present from birth. Multiple studies have shown that mutations in genes involved in heart development could cause congenital heart disease. The CITED2 gene works as a transcription factor in the hypoxic pathway for the development of the heart. Therefore, five CHD types, ventricular septal defect, atrial septal defect, atrioventricular septal defect, tetralogy of Fallot, and patent ductus arteriosus, were evaluated by conducting a targeted single nucleotide polymorphism (SNP) analysis of the CITED2 gene variant rs375393125 (T > C). This study aimed to identify the association of CITED2 gene mutations in CHD patients.
Methods
A total of 350 samples were collected 250 from patients and 100 from controls for genetic analysis. Allele-specific PCR and gel electrophoresis were used to identify the target missense mutations. The genotypic results of the CHDs were further validated through Sanger sequencing.
Results
The frequency of the homozygous mutant (CC) in CHD patients was 48.4% and of heterozygous mutant (TC) genotype was 11.4%; these percentages are higher than controls (1%). The control samples had only one heterozygous TC and no homozygous CC genotype. The chi-square value was obtained at 103.9 with a probability of 0.05, which is greater than the significance value of 21.03. The odds ratio was 43.7, which is > 1. The calculated value of ANOVA was 11.6, which was more significant than the F critical value of 3.7. As a result of sequencing, the mutant sample of each selected CHD type was found heterozygous or homozygous and the results were like as those obtained through conventional PCR.
Conclusion
CHD patient’s samples showed mutations. Therefore, it can say that CITED2 gene SNP might be associated with CHD.
Four-year effectiveness, safety and drug retention rate of secukinumab in psoriatic arthritis: a real-life Italian multicenter cohort
Authors: Ramonda Roberta , Lorenzin Mariagrazia , Chimenti Maria Sole , Atzeni Fabiola , Semeraro Angelo , D’Angelo Salvatore , Selmi Carlo , Ortolan Augusta , Marchesoni Antonio , Manara Maria , Gentiloni Michele Maria Luchetti , Santo Leonardo , Salvarani Carlo , Cauli Alberto , Rossini Maurizio , Amato Giorgio , Cozzi Giacomo , Scagnellato Laura , Ferraioli Mario , Carriero Antonio , Fracassi Elena , Giorgio Francesco , Doria Andrea , Foti Rosario , Carletto Antonio , Spadaro” Spondyloartritis and Psoriatic Arthritis SIR Study Group “An
Published: March 2024

Objectives
to evaluate over a 48-month follow-up period the: 1) long-term effectiveness and safety; 2) drug retention rate (DRR); 3) impact of comorbidities and bDMARDs line on MDA and DAPSA remission/low disease activity (LDA) of secukinumab in a multicenter Italian cohort of PsA patients.
Methods
Consecutive PsA patients receiving secukinumab were followed prospectively in Italian centers between 2016 and 2023. Disease characteristics, previous/ongoing treatments, comorbidities and follow-up duration were collected. Treatment response was evaluated at 6 and 12 months after initiation, and every year up to 48 months (T48). DRR was assessed according to clinical and demographic features, comorbidities and bDMARDs line. Adverse events (AE) were recorded.
Results
685 patients [42.5% male] were enrolled; 32.9% naïve received secukinumab; 74.2% had ≥ 1 comorbidity. Overall, secukinumab yielded improved outcomes at T48: naïve maintained lower disease activity vs. non-naïve [DAPSA 4.0 (1.4–8.1) vs. 6.0 (2.2–10.4);p = 0.04]; 76.9% naïve and 66.2% non-naïve achieved MDA; MDA no comorbidities vs. 1–3 comorbidities 78.8% vs. 73.3% (p < 0.05), and MDA no comorbidities vs. >3 comorbidities 78.8% vs. 48.7% (p < 0.001). DAPSA-REM and DAPSA-LDA rates were higher in naïve patients, albeit similar between those without comorbidities vs. 1–3 comorbidities, and slightly higher in those with > 3 comorbidities. Treatment was discontinued in 233 patients due to loss of effectiveness, and in 41 due to AE. The overall DRR at T48 was 66%, with differences according to bDMARDs line, use of combined csDMARDs (p = 0.016), and mono/oligoarthritis vs. polyarthritis; p = 0.012.
Conclusions
Secukinumab proved safe and effective, and patients achieved sustained remission with a notable drug retention rate at 4 years.
Enhancing flame retardancy, thermal stability, and superhydrophobic of paper through Na₂O·nSiO₂ and PDMS modification
Authors: Jiang Xiawang , Sun Delin , Yu Minggong , Zhao Shan , Song Ling
Published: March 2024

The water absorption and flammability of paper pose significant challenges for its long-term effectiveness. In addressing these concerns, a paper modification strategy was proposed that synergistically incorporated superhydrophobicity and flame retardancy. This approach involved the formation of a micro or nano-level rough structure on the paper surface through sodium silicate modification. Subsequent modification with polydimethylsiloxane (PDMS) resulted in a functional paper, exhibiting excellent superhydrophobic properties. The water contact angle (WCA) and sliding angle (SA) of the modified paper reached 153.5° and 9°, respectively, exhibiting excellent self-cleaning ability and wear resistance. The results from TG–DTG analysis and cone calorimeter tests indicated that the superhydrophobic paper exhibited remarkable thermal stability and flame-retardant properties. These properties contributed to enhancing the safety of products during application. The comprehensive improvement in paper properties, including its superhydrophobicity, flame retardancy, and thermal stability, holds significant implications for expanding its application range and enhancing its overall utility.
Deep Learning for Outage Probability Minimization in Secure NOMA Energy Harvesting UAV IoT Networks
Authors: Nguyen Quoc-Long , Dang Viet-Hung , Nguyen Gia Nhu , Nguyen Trong Thanh , Ho Dac Tu , Tran Hung , Tran Duc-Dung , Le Thanh Cong , Vo Nhan Van
Published: March 2024

In this paper, a secure uplink non-orthogonal multiple access (NOMA) internet of things (IoT) system using an energy harvesting unmanned aerial vehicle (UAV) is studied. The communication protocol includes three phases: The first phase is an energy harvesting phase in which the IoT devices and UAV relay harvest radio frequency energy from a power beacon. The second phase is information transmission from IoT devices to a UAV relay. The third phase is relaying transmission from the UAV relay to the base station. Furthermore, a UAV eavesdropper (UE) wiretaps the signals from the IoT devices and UAV relay in the second and third phases. For this system, we derived the closed-form outage and intercept probabilities to evaluate the system and secrecy performances. The constrained optimization algorithm for minimizing the outage probability is then deployed to obtain training data. Finally, a deep learning model is trained to predict the optimal configuration parameters, enabling rapid adaptation to environmental conditions.
Effect of apigetrin in pseudo-SARS-CoV-2-induced inflammatory and pulmonary fibrosis in vitro model
Authors: Han Hengmin , Kim Jung-Eun , Lee Hyo-Jeong
Published: March 2024

SARS-CoV-2 has become a global public health problem. Acute respiratory distress syndrome (ARDS) is the leading cause of death due to the SARS-CoV-2 infection. Pulmonary fibrosis (PF) is a severe and frequently reported COVID-19 sequela. In this study, an in vitro model of ARDS and PF caused by SARS-CoV-2 was established in MH-S, THP-1, and MRC-5 cells using pseudo-SARS-CoV-2 (PSCV). Expression of proinflammatory cytokines (IL-6, IL-1β, and TNF-α) and HIF-1α was increased in PSCV-infected MH-S and THP-1 cells, ARDS model, consistent with other profiling data in SARS-CoV-2-infected patients have been reported. Hypoxia-inducible factor-1 alpha (HIF-1α) siRNA and cobalt chloride were tested using this in vitro model. Furthermore, apigetrin, a glycoside bioactive dietary flavonoid derived from several plants, including Crataegus pinnatifida, which is reported to be a HIF-1α inhibitor, was tested in this in vitro model [1]. Apigetrin significantly reduced the increased inflammatory cytokine (IL-6, IL-1β, and TNF-α) expression and secretion by PSCV in MH-S and THP-1 cells. Apigetrin inhibited the binding of the SARS-CoV-2 spike protein RBD to the ACE2 protein. An in vitro model of PF induced by SARS-CoV-2 was produced using a conditioned medium of THP-1 and MH-S cells that were PSCV-infected (CMPSCV) into MRC-5 cells. In a PF model, CMPSCV treatment of THP-1 and MH-S cells increased cell growth, migration, and collagen synthesis in MRC-5 cells. In contrast, apigetrin suppressed the increase in cell growth, migration, and collagen synthesis induced by CMPSCV in THP-1 and MH-S MRC-5 cells. Also, compared to control, fibrosis-related proteins (CTGF, COLA1, α-SMA, and HIF-1α) levels were over two-fold higher in CMPSV-treated MRC-5 cells. Apigetrin decreased protein levels in CMPSCV-treated MRC-5 cells. Thus, our data suggest that hypoxia-inducible factor-1 alpha (HIF-1α) might be a novel target for SARS-CoV-2 sequela therapies and apigetrin, representative of HIF-1alpha inhibitor, exerts anti-inflammatory and PF effects in PSCV-treated MH-S, THP-1, and CMPVSC-treated MRC-5 cells. These findings indicate that HIF-1α inhibition and apigetrin would have a potential value in controlling SARS-CoV-2-related diseases.
Multi-environment Clonal Selection Using Ideotype-Design Derived From Factor Analytic Linear Mixed Models: An Application on Eucalyptus Breeding
Authors: Barros Lenin Pereira , Marcal Tiago Souza , Goncalves Flavia Maria Avelar , Nunes Jose Airton Rodrigues , Carneiro Vinicius Quintao , Botelho Thiago Tavares , Aguiar Aurelio Mendes , Lima Jose Luis , Dias Donizete da Costa
Published: March 2024

The growing demand for raw materials in the forestry sector and the imperative to conserve native forests have spurred the expansion of forest areas planted globally, particularly in Brazil. Eucalyptus stands out as the primary genus employed in the Brazilian forestry industry. Addressing the need for new clones due to expanding exploitation and changing climate conditions poses a considerable challenge. The evaluation of genotypes in multi-environment trials (MET) is complicated by their varying behavior. Factor analytic mixed models (FAMM) are presently employed for MET analyses, enabling the modeling of genotype-by-environment interactions without a substantial increase in parameters. However, existing tools for assessing genotype stability and adaptability within FAMM are unsuitable for scenarios involving a large number of eucalyptus clones, typical in intermediate clonal testing stages. To address this gap, a methodology for clonal selection in eucalyptus is required. FAMM offers a way to summarize clone responses across environments through scores. Utilizing the genotype-ideotype (GI) distance based on these scores facilitates the ranking of clones. This study aims to propose and assess the use of genotype-ideotype distance in conjunction with factor analytic mixed models for selecting eucalyptus clones in multi-environment trials. The combined use of factor analytic mixed models and genotype-ideotype distance enables the ranking of clones based on broad adaptability. This approach balances the simplicity of a single parameter's interpretability with the capability to handle a large number of clones effectively.
Exploring Eco-friendly BaZrS3 Chalcogenide Perovskite Solar Cells with Earth-abundant Charge Transport Materials
Authors: Sadek Samiul , Sobayel K. , Alghafis Abdullah
Published: March 2024

This study investigates the efficacy of BaZrS3, an emerging chalcogenide perovskite material, by conducting a comparative analysis of its opto-electronic performance using different charge transport layers. The research juxtaposes the conventional TiO2 and Spiro-OMeTAD layers against earth abundant alternatives like gallium-doped zinc oxide (GZO) and CuSbS2. Results indicate that the configuration FTO/GZO/BaZrS3/CuSbS2 markedly surpasses the FTO/TiO2/BaZrS3/Spiro-OMeTAD arrangement in terms of photovoltaic performance metrics. The suggested structure exhibits an energy conversion efficiency of roughly 20%, surpassing the approximately 11% efficiency achieved by the conventional device structure relying on traditional charge transport layers. This research demonstrates that the absorber layers' thickness and defect density are critical in defining the device's performance, with the highest efficiency attained when the thickness of the structure is maintained below 1 µm. A bulk defect density ranging from 1x1014 /cm3 to 1x1016 /cm3 is found to be conducive to high device performance. Additionally, the doping concentration in each charge transport layer is found to play a pivotal role on the overall device performance. Finally, the study paves the way for chalcogenide perovskites for photovoltaic applications.
LncRNA H19 was a prognostic biomarker associated with immune infiltrates in stomach adenocarcinoma
Authors: QIU WEI , Hu Wei , Chen SuMei , Liu DongSheng
Published: March 2024

Background
Expression of long non-coding RNA (LncRNA) H19 was observed in many diseases, while its role in stomach adenocarcinoma (STAD) was unclear. The objective of this research was to demonstrate the relationship between H19 and STAD.
Methods
Kaplan-Meier (K-M) analysis was applied to analyze the variations in survival of different subgroups of H19 expression. Immunoanalysis and gene set enrichment analysis (GSEA) were utilized to filter the H19 functions that were substantially implicated. The methodology of Cox regression analysis was adopted to assess factors contributing to the prognosis. we also established an H19 ceRNA network and performed drug sensitivity analysis. In the final phase, the consistency between the expression of H19 in clinical samples and the results of bioinformatics was verified by RT-qPCR.
Results
High H19 expression predicted poorer survival, and plasmacytoid dendritic cells, neutrophils, and activated CD4 and CD8 T cells were correlated with the prognosis of STAD patients. The immune infiltration and GSEA analysis hypothesized that H19 expression was connected to the function of ECM receptor intraction and some differential immune cells. Independent prognostic analysis suggested that age, tumor stage, and H19 expression could act as separate prognostic factors. Ultimately, we constructed the ‘H19-3 miRNA-18 mRNA’ network. RT-qPCR results showed that the expression level of H19 in STAD tissues was significantly higher than that in adjacent normal tissues.
Conclusion
H19 expression was significantly connected to poor survival in STAD, and it might be a prospective biomarker for prognosis in STAD.
A granularity time series forecasting model combining three-way decision and trend information granularity
Authors: Qiu Jianuan , Su Shuhua , Qian Jingjing
Published: March 2024

In recent decades, significant advancements have been made in the field of time series data mining, leading to its widespread application in various domains. However, the existence of data correlation in time series data sets brings challenges to long-term prediction. One approach to address this issue is to transform the original time series into granular time series (GTS). Therefore, a prediction model based on GTS is proposed to meet this challenge in this study. Firstly, the improved L1-trend filtering is used to achieve the optimal segmentation of information particles. Then, a three-way decisions (TWD) model based on shape similarity is used to compress and aggregate information granules. Finally, a GTS prediction model based on LSTM neural network is established. The model effectively retains the trend information of the time series and overcomes the limitation that the existing models cannot adjust the granularity length of the original information. In addition, the proposed model is applied to several real datasets for sensitivity analysis and comparative analysis. The results show that the model has strong performance in long-term forecasting.
Matrine inhibits invasion and migration of gallbladder cancer via regulating the PI3K/AKT signaling pathway
Authors: Mo Rong-Liang , Li Zhuang , Zhang Peng , Sheng Ming-Hui , Han Gen-Cheng , Sun Deng-Qun
Published: March 2024

Gallbladder cancer (GBC) is a common malignant cancer in the biliary system, which poses a serious threat to human health. It is urgent to explore ideal drugs for the treatment of GBC. Matrine is the main active ingredient of sophora flavescentis, with a wide range of biological activities encompassing anti-inflammatory, antiviral, immunomodulatory and anti-tumor. However, the underlying mechanism by which Matrine treats GBC is still unclear. The purpose of this study is to investigate the anti-tumor effects of Matrine on GBC in vivo and in vitro, and to clarify the potential regulatory mechanisms. Here, in this primer, we found that Matrine has a significant killing effect on GBC through CCK8 and flow cytometry, including arrest of cell cycle, inhibition of GBC cell, and induction of apoptosis. Further studies in vivo confirmed that the inhibitory function of Matrine on tumor growth in NOZ xenografted nude mouse. At the same time, Matrine also significantly suppressed the migration and invasion of GBC cells through scratch and Transwell experiments. In addition, by detecting the mRNA and protein levels of epithelial-mesenchymal transition (EMT) and matrix metalloproteinases, Matrine furtherly substantiated the suppression of invasion and migration of GBC. From a mechanistic perspective, Matrine effectively decreased the abundance of p-PI3K and p-AKT protein in vivo and in vitro. More importantly, PI3K activator (740 Y-P) antagonized the anti-tumor effect of Matrine, while PI3K inhibitor (LY294002) increased the sensitivity of Matrine for GBC. Based on the above findings, we conclude that Matrine inhibits the invasion and migration of GBC by regulating PI3K/AKT signaling pathway. Our results indicate the crucial role and regulatory mechanism of Matrine in suppressing the growth of GBC, which provides a theoretical basis for Matrine to be a candidate drug for the treatment and research of GBC.
Multiperiod vaccination facility planning
Authors: Tacadao Grace , Simonis Helmut , Quesada Luis , O'Sullivan Barry
Published: March 2024

We propose a decision support tool, based on Mixed Integer Programming, to help with the planning of national vaccination campaigns, and test it on data and scenarios obtained from the publicly available information about the Irish COVID-19 vaccination program. We show that a large variety of constraints can be included in the tool, as well as combinations of different objective functions for the different stakeholders. The tests show that the multi-period/multi-vaccine problem can be effectively solved by the given model, while allowing to consider the impact of potential changes in the input data. We also present a number of specific visualizations that help the user understand the plan created by the application.
Magnitude of heterosis for economic traits in newly developed single hybrids of Silkworm, Bombyx mori L.
Authors: shinde Shriram Tukaram
Published: March 2024

An investigation was conducted in India using the parental stock breeds among the six bivoltine tester and five multivotine line strains of the silkworm Bombyx mori L., to estimate heterosis expressed in single cross hybrids with respect to important economic traits. In terms of mean value performance, testers CSR50 and GEN2, and line BL67, showed the most promise, while the F1 hybrid BL67 × GEN2 produced the highest shell weight, cocoon-shell percentage and maximum cocoon-filament weight. Other superior hybrids were BL24 × GEN2 for single cocoon weight, PM × GEN2 for cocoon filament length and HM × CSR17 for denier. For each trait in the hybrid combination, different heterotic effects were noted. Based on heterosis, hybrid BL67 × GEN2 was optimal for cocoon-shell ratio and single shell weight; PM × GEN2 for cocoon filament length and weight; PM × CSR16 for single cocoon weight; and BL24 × CSR5 for Denier. The majority of the hybrids outperformed the BL67 × CSR5 standard check variety. For all the characters studied collectively, no one hybrid outperforms the others. In general, the traits studied exhibited a greater degree of heterosis in their crosses. The overall analysis unequivocally demonstrates that future breeding programs will use the high-yielding lines and testers that were identified as potential breeds for the synthesis of superior multi × bivoltine hybrids for tropical conditions.
RETRACTED: Heatwave Induced Health Vulnerability Assessment in Bangladesh
Authors: Mehrin Maria , Zuhra Fatema Tuz , Rahman Md. Mafizur
Published: March 2024

The authors have requested that this preprint be removed from Research Square.
A critical discourse analysis of rejoinders to political press releases in selected Nigerian newspapers
Authors: JIDO ALIYU ABDULKAREEM
Published: March 2024

This paper presents a Critical Discourse Analysis of rejoinders to political press releases in selected Nigerian nnewspapers. The study examined the linguistic and discursive features, power relations and ideological positioning, and the contributions of rejoinders in shaping political narratives and public opinion. The analyses were based on Fairclough’s three dimensional approach to CDA.
The framework identified three approaches to CDA, namely: texts, discourse practice and sociocultural context. The approach pave way for systematic analysis of the data, and also help in arriving at the findings of the study. Data for the study comprises of ten (10) rejoinders from the selected Nigerian newspapers.
The selected Nigerian newspapers are: Punch Newspaper, Vanguard Newspaper, The Guardian Newspaper, The Blueprints Newspaper, TheCable Newspaper, DailyTrust Newspaper. Rejoinders analyzed are those from June 2022 to June 2023. Data were purposefully chosen on a specific genre of rejoinders to political press releases, such as statements from political parties, government officials, or opposition groups, providing a focused perspective. Furthermore, the criteria for selecting the sample of rejoinders, includes; relevance to political issues, diversity of perspectives, and prominence of the author.
This approach facilitates an in-depth exploration of linguistic features, power dynamics, and ideological positioning within political rejoinders.
The findings of this study reveals the strategic use of language in political rejoinders, covering vocabulary precision, power relations, ideological positioning, and rhetorical strategies.
In conclusion, this research contributes valuable insights to the field of CDA, emphasizing intertextuality, transparency, and accountability in political discourse. It serves as a valuable resource for academics and provides practical insights for enhancing political communication strategies.
First insight into the genomes of the Pulmonaria officinalis group (Boraginaceae) provided by repeatome analysis and comparative karyotyping
Authors: Kobrlová Lucie , Čížková Jana , Zoulová Veronika , Vejvodová Kateřina , Hřibová Eva
Published: March 2024

Background
The genus Pulmonaria (Boraginaceae) represents a taxonomically complex group of species in which morphological similarity contrasts with striking karyological variability. The presence of different numbers of chromosomes in the diploid state suggests multiple hybridization/polyploidization events followed by chromosome rearrangements (dysploidy). Unfortunately, the phylogenetic relationships and evolution of the genome, have not yet been elucidated. Our study focused on the P. officinalis group, the most widespread species complex, which includes two morphologically similar species that differ in chromosome number, i.e. P. obscura (2n = 14) and P. officinalis (2n = 16). Ornamental cultivars, morphologically similar to P. officinalis (garden escapes), whose origin is unclear, were also studied. Here, we present a pilot study on genome size and repeatome dynamics of these closely related species in order to gain new information on their genome and chromosome structure.
Results
Flow cytometry confirmed a significant difference in genome size between P. obscura and P. officinalis, corresponding to the number of chromosomes. Genome-wide repeatome analysis performed on partial Illumina sequencing data showed that retrotransposons were the most abundant repeat type, with a higher proportion of Ty3/Gypsy elements, mainly represented by the Tekay lineage. Comparative analysis revealed no species-specific retrotransposons or striking differences in their copy number between the species. A new set of chromosome-specific cytogenetic landmarks, represented by satellite DNAs, showed that the chromosome structure in P. officinalis was more variable compared to that of P. obscura. Comparative karyotyping strongly supported the hybrid origin of putative hybrids with 2n = 15 collected from a mixed population of both species and outlined the origin of ornamental garden escapes, confirming their derivation from the P. officinalis complex.
Conclusions
Large-scale genome size analysis and repeatome characterization of the two morphologically similar species of the P. officinalis group improved our knowledge of the genome dynamics and differences in the karyotype structure. A new set of chromosome-specific cytogenetic landmarks was identified and used to reveal the origin of putative hybrids and ornamental cultivars morphologically similar to P. officinalis.
MEWUJUDKAN PARTAI POLITIK YANG DEMOKRATIS MELALUI SISTEM PRIMARY ELECTIONS DI INDONESIA
Authors: Oktasari Frenty , Razzak Abdul , Liza Fransisca Bella
Published: March 2024

Di Negara Indonesia pemilihan calon presiden dan wakil presiden yang terdapat pada Partai Politik terlihat kurang sistematis dan demokratis. Dimana pengambilan keputusan hanya berdasar kepada ketua umum partai politik saja. Sehingga menyebabkan calon presiden dan wakil presiden yang dipilih ketua umum partaiv, sering kali tidak sesuai kehendak dan terkesan konstituen. Terdapat beberapa Partai Politik yang mengelola hal tersebut dalam anggaran dasar, akan tetapi ketentuan yang dimaksud tidak mampu melakukanya dengan maksimal yang di sebabkan karena adannya peraturan presidential threshold yang memakssa setiap partai untuk berkoalisi untuk mencalonkan sebagai presiden dan wakil presiden. Yang mana problem tersebut mampu di atasi karena adanya pengaplikasian primary elections seperti yang pernah dilakukan oleh negara-negara demokratis seperti negara Amerika Serikat dan juga negara Prancis. Tujuan dari jurnal penelitian ini untuk mengasumsikan pengaplikasian primary elections di Negara Indonesia dalam mewujudkan Partai Politik yang demokratis. Dalam  penelitia ini, artikel yang digunakan di ambil dari sumber-sumber yang relevaan untuk menjelaskan argumentasi penting terkait dengan pengaplikasian primary elections di negara Indonesia. Selain itu artikel juga menggunakan studi komparasi dengan negara Perancis dan negara Amerika Serikat sebagai negara yang telah menerapkan primary elections. Dengan melihat urgensi menerapkan primary elections sangat perlu diterapkan di negara Indonesia ini. Karena searah dengan tujuan dari regulasi, yang mana sebagai salah satu upaya agar terwujudnya perlembagaan Partai Politik yang demokratis dengan mekanisme yang di ambil pada pemilihan calon presiden dan calon wakil presiden yang dipilih langsung oleh pemilih.
Anomalous Luttinger equivalence between temperature and curved spacetime: From black holes to thermal quenches
Authors: Bermond Baptiste , Chernodub Maxim Nikolaevich , Grushin Adolfo G. , Carpentier David
Published: March 2024

Building on the idea of Tolman and Ehrenfest that heat has weight, Luttinger established a deep connection between gravitational fields and thermal transport. However, this relation does not include anomalous quantum fluctuations that become paramount in strongly curved spacetime. In this work, we revisit the celebrated Tolman-Ehrenfest and Luttinger relations and show how to incorporate the quantum energy scales associated with these fluctuations, captured by gravitational anomalies of quantum field theories. We point out that such anomalous fluctuations naturally occur in the quantum atmosphere of a black hole. Our results reveal that analogous fluctuations are also observable in thermal conductors in flat-space time provided local temperature varies strongly. As a consequence, we establish that the gravitational anomalies manifest themselves naturally in non-linear thermal response of a quantum wire. In addition, we propose a systematic way to identify thermal analogues of black hole’s anomalous quantum fluctuations associated to gravitational anomalies. We identify their signatures in propagating energy waves following a thermal quench, as well as in the energy density of heating Floquet states induced by repeated thermal quenches.
OPHTHALMOLOGIC MANIFESTATIONS REVEALING POSTERIOR REVERSIBLE ENCEPHALOPATHY SYNDROME: A CASE REPORT
Authors:   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Boutaj Taha , Sbai Latifa ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Lazaar Hamza ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Benkirane Romaissae ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Tabchi Manal ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Hilali Zineb ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., El Hachimi Rim ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Tachfouti Samira ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Amazouzi Abdellah ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco., Cherkaoui Ouafa ,   Ophthalmology Department A, Ibn Sina University Hospital (Hopitaldes Specialites), Mohammed V University, Rabat, Morocco.
Published: March 2024

Posterior reversible encephalopathy syndrome (PRES) is a rare clinico-radiological syndrome, secondary to many diseases especially high blood pressure. First described by Hinchey in 1996, pathophysiology is not clear. Clinical manifestations are headache, seizures, altered mental state such as confusion or coma, and also visual disturbances, such as hemianopia, blurred vision, diplopia, or cortical blindness. We report the case of a Moroccan 47-years old patient who arrived at the ophthalmology emergency presenting a sudden vision loss and horizontal diplopia, associated with headache. General examination found a high blood pressure at 240/110mmhg. The funduscopy of both eyes found hypertensive retinopathy. A brain MRI was realized showing bilateral occipital cortex T2/fluid-attenuated inversion recovery (FLAIR) hyperintensities. The diagnosis of Posterior reversible encephalopathy syndrome was retained.
CLINICAL SIGNIFICANCE AND IMPACT OF FUNGAL AND BACTERIAL INFECTIONS IN HUMAN HEALTH
Authors:   Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India., Lakshmi Evuri Yoshitha , Shree Gurram Gnana ,   Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India., Lakshmi Devi Tumati Jhansi ,   Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India., Challagundla Mounika ,   Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India., Ganta Varshanjali ,   Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India., Sreeram Devi ,   Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India., Naga Lakshmi Sriprada Pernamitta Sai ,   Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India., Priya Amarthaluri Santhi ,   Department of Biotechnology, Sri Venkateshwara College of Pharmacy, Madhapur, Hyderabad, Telangana, India.
Published: March 2024

Our study aims to explore the origins of infectious diseases and the responses of the human body to invading pathogens. Despite the development of antibiotics and vaccines, the threat of infection remains ever-present. Continuously emerging pathogens and antibiotic-resistant strains pose challenges to effective treatment, while diseases such as AIDS and rabies remain incurable. While infectious diseases have been recognized for millennia, comprehensive understanding of their causes has only emerged in the last century. We focus on elucidating the diverse array of disease-causing organisms and the underlying mechanisms that drive clinically apparent infections. This knowledge is essential for individuals and serves as a fundamental requirement for the diagnosis, treatment, and prevention of infectious diseases.
EFFECT OF LOW LEVEL LASER OF 650NM ON VIALBILITY OF PREVOTELLA INTERMEDIAWITH AND WITHOUT PHOTOSENSITISERS: AN IN VITRO STUDY
Authors:   Professor and Head of the Department, Department of Periodontology, KVG Dental College and Hospital, India., Dayakar M.M , Nihal Abbas Majeed Mohammed ,   Post Graduate Student, Department of Periodontology, KVG Dental College and Hospital, India., Gurulingappa Apoorva ,   Post Graduate Student, Department of Periodontology, KVG Dental College and Hospital, India.
Published: March 2024

Background: Prevotella intermedia is commonly associated with various forms of periodontal disease. Conventional treatment involves a combination of mechanical therapy and systemic antibiotics administration, which comes with its own set of challenges and disadvantages. An alternative treatment modality is low-level laser therapy with and without photosensitizers. Purpose: This study aimed to evaluate the effectiveness of low-level laser therapy with and without the use of photosensitizers, specifically Methylene blue and Toluidine blue, on the viability of Prevotella intermedia. Methods: The study utilized in vitro experiments to assess the impact of low-level laser therapy alone and in combination with photosensitizers on the viability of Prevotella intermedia. Results: The findings indicate that Methylene blue, when combined with low-level laser therapy, exhibits bactericidal effects similar to or greater than low-level laser therapy alone on Prevotella intermedia. Additionally, the use of Toluidine blue as a photosensitizer in combination with low-level laser therapy also led to a reduction in the viability of Prevotella intermedia, although it was not as effective as Methylene blue. Conclusion: Low-level laser therapy, particularly when combined with Methylene blue as a photosensitizer, shows promise as a potential treatment modality for controlling Prevotella intermedia in periodontal diseases.
Estudo bibliométrico sobre Desdobramento da Função Qualidade (QFD) no desenvolvimento de produtos
Authors: Genauch Carlos Davi , Pechibilski Nilton Willian , Favretto Jacir , Silva Robson de Faria , Witt Cleonice
Published: March 2024

O Processo de Desenvolvimento de Produto (PDP) é fundamental para a estratégia de empresas se manterem no mercado, e o Desdobramento da Função Qualidade (QFD) é um método que reúne características desejadas pelos clientes e disponibilidades técnicas pela empresa para desenvolver e aprimorar a qualidade do produto e/ou serviço, visando à percepção do cliente e produtos com qualidade e aceitação do público. O presente estudo objetivou verificar as publicações e a atualidade da temática sobre o Desdobramento da Função Qualidade na Gestão de Desenvolvimento de Produtos. Como metodologia de pesquisa, foram empregadas técnicas de revisão sistemática de literatura e das leis da bibliometria de Zipf, Lotka e Bradfort. As análises de resultados foram, após as consultas, as bases de dados científicas, Spell, Scielo e EBSCO, sendo considerados 35 artigos relevantes para esta pesquisa, compreendendo o período de 1996 até 2022 e distribuídos em 19 periódicos e universo de 133 autores. Como considerações importantes, esta pesquisa observou baixo volume de artigos focados na prática e nos resultados do QFD em detrimento de artigos focados nas características e implementação do método, podendo servir de base para prospecção de novos estudos e podendo inspirar pesquisas futuras.
O projeto do polo tecnológico do mar da Baía de Sepetiba (PTM-BS): Possibilidades e probabilidades de participação do Colegiado BIG
Authors: Cardoso Rafaela Rosa Chaves , Maury Patrick Maurice , De Carvalho Isabella Dias , Lima Nicholas Augusto Mendes da Rocha , Villela Lamounier Erthal
Published: March 2024

O Polo Tecnológico do Mar da Baía de Sepetiba (PTM-BS) está relacionado com a implementação da base de Submarinos Nucleares pela Marinha do Brasil. A referência é o Pôle Mer Bretagne Atlantique da França. Este modelo foi adotado no Brasil com o intuito de mitigar os impactos socioambientais do Complexo Naval de Itaguaí - ICN e de outros megaempreendimentos implantados no mesmo território, como a Vale, o Porto Sudeste e a Nuclep. Neste território, atua o Colegiado Territorial Rural da Baía da Ilha Grande (Colegiado BIG) apoiado pelo Programa de Ensino, Pesquisa e Extensão em Desenvolvimento Territorial e Políticas Públicas, da Universidade Federal Rural do Rio de Janeiro (PEPEDT/UFRRJ. O motivo que levou a construir os cenários do Polo Tecnológico do Mar da Baía de Sepetiba (PTM-BS) na dissertação de mestrado da primeira autora, é porque além do projeto do PTM-BS não ter um projeto consolidado e em execução, o projeto do PTM-BS demonstra ser contrário aos interesses do Colegiado BIG de um possível pacto pela sustentabilidade em termos de Governança. Os cenários resultaram da combinação dos parâmetros de inovação tecnológica, investimento/financiamento e organização/governança do território. Foram obtidos oito cenários e consideradas duas variáveis para cada parâmetro, características das lógicas vertical das cadeias produtivas e horizontal dos painéis de indicadores (dashboard) de sustentabilidade. Este artigo baseia-se na dissertação de mestrado da primeira autora, na qual foi verificada a impossibilidade de acessar as fontes de informação sobre investimentos públicos previstos para quantificar as variáveis deste parâmetro. A proposta alternativa, apresentada e analisada neste artigo, utiliza uma abordagem relacional do parâmetro governança, qualificando a influência da percepção dos agentes envolvidos em relação aos 17 Objetivos do Desenvolvimento Sustentável - ODS das Nações Unidas. Finalmente, considera as possibilidades e probabilidades de o PTM-BS vir a adotar os cenários apontados como mais efetivos para a sustentabilidade do território em discussão.
Extensión universitaria y financiación de proyectos relacionados con la Educación Física en la Universidad Federal de Mato Grosso do Sul
Authors: Costa Emanuele Vasconcelos da , Albuquerque Alex Caiçara de , Silva Junior Vagner Pereira da
Published: March 2024

Aimed to investigate the funding of extension projects in the context of the Federal University of Mato Grosso do Sul, Brazil. It is an exploratory, documentary, longitudinal, retrospective, quantitative research. The object of analysis were public funding edicts (2010-2021) of extension projects, and the values in reais corrected by the National Consumer Price Index. A total of 1.535 extension projects were financed, standing out 2010 (13.49%) and 2017 (11.96%) in number of projects, with a decline from 2018. Regarding the amounts funded, 2017 (R$799,046.69) and 2018 (R$909,244.62) showed the best results and 2019 (R$317,075.88) and 2021 (R$77,099.48), a significant decline. Regarding the themes linked to Physical Education, 5.60% projects were contemplated, highlighting the theme sport (37.10%) when compared to health, leisure physical activity and leisure. It is concluded that the funding of extension projects went through oscillations, and the themes related to Physical Education were present, although in small percentage.
Composite or Modified Hydroxyapatite Microspheres as Drug Delivery Carrier for Bone and Tooth Tissue Engineering
Authors: Wang Zhe , Shang Jiaxin , Zhang Zheng
Published: March 2024

Since hydroxyapatite (HAp) is an important constituent of bone and teeth, it
has excellent biocompatibility and bioactivity, good osteoconductive effects and the ability
to induce bone formation as a material for bone or tooth repair and replacement. At
present, widely used HAp microspheres have some characteristics, such as large specific
surface area, light mass, good injection properties, good fluidity, and low aggregation
ability, but they are difficult to really meet the biological and clinical needs due to their
own mechanical property defects, such as low strength, brittleness, and poor plasticity.
Based on the current research status of HAp microspheres, we summarize the research
progress of various types of composite microspheres, including inorganic materials, natural
polymer materials and synthetic polymer materials, and further analyze the advantages
of HAp composite microspheres loaded with drug molecules, proteins and bioactive
factors, so as to explore the development prospect of HAp composite microspheres
as scaffolds for constructing sustained release systems. It provides a theoretical basis
and research direction to prepare HAp composite micro-spheres with superior comprehensive
properties so that they can be better applied in bone tissue regeneration and
tooth regeneration engineering.
Multicomponent 3D-printed Collagen-based Scaffolds for Cartilage Regeneration:
Recent Progress, Developments, and Emerging Technologies
Authors: Mikaeeli Kangarshahi Babak , Naghib Seyed Morteza
Published: March 2024

Cartilage tissue presents challenges in terms of repair and regeneration due to its inherent limitations in self-healing and the scarcity of available donors. Cartilage damage can result in the development of joint problems characterized by symptoms, such as pain, swelling, and osteoarthritis. Collagen scaffolds are extensively used as biomimetic substances for cartilage engineering due to their ability to offer structural, biochemical, and mechanical signals for chondrocytes. Nevertheless, traditional techniques for producing collagen scaffolds frequently yield inadequate pore architecture, diminished mechanical robustness, and restricted form accuracy. Hence, 3D printing is a developing method that can surpass these restrictions by allowing accurate manipulation of the shape, porousness, and makeup of the scaffold. 3D printing has the capability to include various materials and cells in the scaffolds, resulting in the production of intricate and personalized tissue structures. This research examines the latest progress in utilizing 3D printing to create collagen scaffolds for the purpose of regenerating cartilage. This text discusses the different sources of collagen, methods of cross-linking, techniques for printing, and strategies for post-processing that are employed to improve the performance of scaffolds. Furthermore, it discusses the difficulties and potential future paths of utilizing 3D printing to create collagen scaffolds for the purpose of regenerating cartilage.
Trends in the Synthesis of Antimicrobial Derivatives by using the Gewald,
Strecker, and Groebke-Blackburn-Bienaymé (GBB) Reactions
Authors: Naithani Kaushal , Bhowmik Subhendu
Published: March 2024

Background:

Multicomponent reactions are highly useful in synthesizing natural products
and bioactive molecules. Out of several MCRs, although utilized widely, some remain neglected
in review articles. The Gewald and Groebke-Blackburn-Bienaymé (GBB) reactions are two such
reactions. This comprehensive review assimilates applications of Gewald and Groebke-Blackburn-
Bienayme reactions in synthesizing novel antimicrobial agents. It presents the antimicrobial properties
of the synthesized molecules, providing an overview of their potential druggability.

Objective:

Developing novel antimicrobial agents is the need of the hour. Toward this objective,
the scientific community is developing new methods for constructing novel architectures with potential
antimicrobial properties. This review will showcase the usefulness of the Gewald, Strecker,
and Groebke-Blackburn-Bienaymé (GBB) reactions in synthesizing antimicrobial molecules.

Methods:

The articles are searched by using the Sci-finder search tool and summarize the chemistry
of their synthesis and antimicrobial evaluation of the molecules

Results:

This review focuses on synthesizing antimicrobial molecules using the Gewald, Strecker,
and Groebke-Blackburn-Bienaymé (GBB) reactions. The antimicrobial activities of the synthesized
molecules are also summarized in tables.

Conclusion:

This review will briefly overview the application of the Gewald, Strecker, and Groebke-
Blackburn-Bienaymé (GBB) reactions in synthesizing novel antimicrobial molecules. It contains
several molecules with promising activity against resistant and non-resistant microbial strains. These
promising molecules could be studied further to develop novel antibiotics.
Comparison of the Efficacy of Oral Magnesium with Oral Ketorolac for Postoperative Pain Management in Anorectal Surgery: A Double-blinded
Randomized Clinical Trial
Authors: Eshagh Hoseini Seyed Jalal , Ghazi Farzanesadat , Eshraghi Mohsen , Vahedian Mostafa , Pashaei Mohammad Reza , Habibi Mohamad Amin , Ahmadpour Sajjad
Published: March 2024

Background:

Due to the presence of postoperative pain in patients undergoing anorectal
surgery, and since the pain affects the quality of life of patients, we aimed to compare the analgesic
effectiveness of oral magnesium with oral ketorolac to choose the right analgesic drug for these
patients.

Methods:

This study was a double-blind, randomized clinical trial performed on 104 candidates
undergoing anorectal surgery. Patients were randomly divided into two groups. Group 1 received
oral magnesium (250 mg daily), and group 2 received oral ketorolac (10 mg daily). The medicine
was given to the patient 2 hours after the operation and every 12 hours for 10 days. Pain measurements
were recorded at 24-hour intervals after surgery based on the visual analog scale and numerical
rating scale.

Results:

This study found that postoperative pain was reduced in patients taking magnesium
tablets, similar to the ketorolac group. A similar decreasing trend was observed in the group receiving
ketorolac; however, the reduction was more pronounced in the magnesium group and was statistically
significant on days 1, 3, and 5 (p < 0.001). However, insignificant differences were noted
between the two groups on the seventh (p = 0.093) and tenth (p = 0.088) postoperative days.

Conclusion:

Taking magnesium tablets after surgery has a suitable analgesic effect, which is similar
to oral ketorolac tablets from the fifth day onwards, but in the initial days, it is less effective
than ketorolac statistically.