bluuebunny/crossref_metadata_2025 huggingface dataset:
Fluorescent Labeling of Peroxisome and Nuclear in Colletotrichum aenigma
Authors: Yu Shendan , Wang Jing , Chai Rongyao , Qiu Haiping , Lu Ziqi , Zhang Zhen , Li Lin , Wang Jiaoyu , Sun Guochang
Published: April 2023

Anthracnose is one of the most widespread and destructive diseases in grapes. Grape anthracnose can be caused by various Colletotrichum species, such as Colletotrichum gloeosporioides and Colletotrichum cuspidosporium. In recent years, Colletotrichum aenigma was reported as a causal agent of Grape anthracnose in China and South Korea. Peroxisome is an important organelle in eukaryotes, which plays a very important role in the growth, development, and pathogenicity of several plant-pathogenic fungal species i, but it has not been reported in C. aenigma. In this work, the peroxisome of C. aenigma was labeled with a fluorescent protein, using green fluorescent protein (GFP) and red fluorescent protein (DsRED and mCherry) as reporter genes. Via Agrobacterium tumefaciens-mediated transformation (AtMT), two fluorescent fusion vectors to mark the peroxisomes, with GFP and DsRED, respectively, were introduced into a wild-type strain of C. aenigma. In the transformants, bright dots of green or red fluorescence in hyphae and spores could be seen in the strains labeled peroxisome. The nuclei labeled by the same method showed bright round fluorescent spots. In addition, we also combined fluorescent protein labeling with chemical staining to show the localization more clearly. The ideal peroxisome and nuclear fluorescence-labeled C. aenigma strain was obtained, which provided a reference for the study of its growth, development, and pathogenicity.
Extracellular Vesicles of the Plant Pathogen Botrytis cinerea
Authors: De Vallée Amelie , Dupuy Jean-William , Moriscot Christine , Gallet Benoit , Vanderperre Solène , Guignard Gaëtan , Rascle Christine , Calvar Glen , Malbert Bastien , Gillet François-Xavier , Dieryckx Cindy , Choquer Mathias , Girard Vincent , Poussereau Nathalie , Bruel Christophe
Published: April 2023

Fungal secretomes are known to contain a multitude of components involved in nutrition, cell growth or biotic interactions. Recently, extra-cellular vesicles have been identified in a few fungal species. Here, we used a multidisciplinary approach to identify and characterize extracellular vesicles produced by the plant necrotroph Botrytis cinerea. Transmission electron microscopy of infectious hyphae and hyphae grown in vitro revealed extracellular vesicles of various sizes and densities. Electron tomography showed the co-existence of ovoid and tubular vesicles and pointed to their release via the fusion of multi-vesicular bodies with the cell plasma membrane. The isolation of these vesicles and exploration of their protein content using mass spectrometry led to the identification of soluble and membrane proteins involved in transport, metabolism, cell wall synthesis and remodeling, proteostasis, oxidoreduction and traffic. Confocal microscopy highlighted the capacity of fluorescently labeled vesicles to target cells of B. cinerea, cells of the fungus Fusarium graminearum, and onion epidermal cells but not yeast cells. In addition, a specific positive effect of these vesicles on the growth of B. cinerea was quantified. Altogether, this study broadens our view on the secretion capacity of B. cinerea and its cell-to-cell communication.
Exploring Differences in Culturable Fungal Diversity Using Standard Freezing Incubation—A Case Study in the Limestones of Lemos Pantheon (Portugal)
Authors: Paiva Diana S. , Fernandes Luís , Pereira Emília , Trovão João , Mesquita Nuno , Tiago Igor , Portugal António
Published: April 2023

In this study, we explored the biodiversity and abundance of culturable fungi in four samples associated with different biodeterioration outlines collected from the Lemos Pantheon, a limestone-built artwork in Portugal. We compared the results from prolonged standard freezing with those previously obtained from fresh samples to analyze differences in the obtained community and assess the effectiveness of the standard freezing incubation protocol in uncovering a different segment of culturable fungal diversity. Our results showed a slight decrease in culturable diversity, but over 70% of the obtained isolates were not present in the previously studied fresh samples. We also identified a high number of potential new species with this procedure. Moreover, the use of a wide variety of selective culture media positively influenced the diversity of the cultivable fungi obtained in this study. These findings highlight the importance of developing new protocols under varying conditions to accurately characterize the culturable fraction in a given sample. The identification and study of these communities and their possible contribution to the biodeterioration process is crucial knowledge for formulating effective conservation and restoration plans to prevent further damage to valuable cultural heritage assets.
Early and Late Response and Glucocorticoid-Sparing Effect of Belimumab in Patients with Systemic Lupus Erythematosus with Joint and Skin Manifestations: Results from the Belimumab in Real Life Setting Study—Joint and Skin (BeRLiSS-JS)
Authors: Zen Margherita , Gatto Mariele , Depascale Roberto , Regola Francesca , Fredi Micaela , Andreoli Laura , Franceschini Franco , Urban Maria Letizia , Emmi Giacomo , Ceccarelli Fulvia , Conti Fabrizio , Bortoluzzi Alessandra , Govoni Marcello , Tani Chiara , Mosca Marta , Ubiali Tania , Gerosa Maria , Bozzolo Enrica P. , Canti Valentina , Cardinaletti Paolo , Gabrielli Armando , Tanti Giacomo , Gremese Elisa , De Marchi Ginevra , De Vita Salvatore , Fasano Serena , Ciccia Francesco , Pazzola Giulia , Salvarani Carlo , Negrini Simone , Di Matteo Andrea , De Angelis Rossella , Orsolini Giovanni , Rossini Maurizio , Faggioli Paola , Laria Antonella , Piga Matteo , Cauli Alberto , Scarpato Salvatore , Rossi Francesca Wanda , De Paulis Amato , Brunetta Enrico , Ceribelli Angela , Selmi Carlo , Prete Marcella , Racanelli Vito , Vacca Angelo , Bartoloni Elena , Gerli Roberto , Zanatta Elisabetta , Larosa Maddalena , Saccon Francesca , Doria Andrea , Iaccarino Luca
Published: April 2023

Aim. To assess the efficacy of belimumab in joint and skin manifestations in a nationwide cohort of patients with SLE. Methods. All patients with skin and joint involvement enrolled in the BeRLiSS cohort were considered. Belimumab (intravenous, 10 mg/kg) effectiveness in joint and skin manifestations was assessed by DAS28 and CLASI, respectively. Attainment and predictors of DAS28 remission (<2.6) and LDA (≥2.6, ≤3.2), CLASI = 0, 1, and improvement in DAS28 and CLASI indices ≥20%, ≥50%, and ≥70% were evaluated at 6, 12, 24, and 36 months. Results. DAS28 < 2.6 was achieved by 46%, 57%, and 71% of patients at 6, 12, and 24 months, respectively. CLASI = 0 was achieved by 36%, 48%, and 62% of patients at 6, 12, and 24 months, respectively. Belimumab showed a glucocorticoid-sparing effect, being glucocorticoid-free at 8.5%, 15.4%, 25.6%, and 31.6% of patients at 6, 12, 24, and 36 months, respectively. Patients achieving DAS-LDA and CLASI-50 at 6 months had a higher probability of remission at 12 months compared with those who did not (p = 0.034 and p = 0.028, respectively). Conclusions. Belimumab led to clinical improvement in a significant proportion of patients with joint or skin involvement in a real-life setting and was associated with a glucocorticoid-sparing effect. A significant proportion of patients with a partial response at 6 months achieved remission later on during follow-up.
Uroperitoneum as a Complication of Laparoscopic Cholecystectomy: A Case Report
Authors: Kefalas Charalampos , Menni Alexandra , Karlafti Eleni , Panidis Stavros , Chatziantoniou Georgios , Katsiafliaka Konstantina , Krokou Despina , Ioannidis Aristeidis , Goulas Patroklos , Netta Smaro , Paramythiotis Daniel
Published: April 2023

Background: Bladder rupture is more frequently encountered in blunt pelvic trauma, but can also be spontaneous or iatrogenic. Laparoscopic repair has been widely used during the last few years as a treatment for intraperitoneal bladder perforation. The bladder is the genitourinary organ most often affected by iatrogenic injury. The purpose of this article is to report what is, to our knowledge, the first documented case of bladder rupture as a complication of laparoscopic cholecystectomy. Case description: A 51-year-old female presented to the emergency department complaining about generalized abdominal pain on the sixth postoperative day after laparoscopic cholecystectomy. Laboratory results highlighted a significant impact on renal function while the abdominal CT scan demonstrated free intraperitoneal fluid collection and surgical clips in the anatomic region of the liver and in an ectopic position near the ileocecal valve. An explorative laparoscopy revealed a 2 cm defect in the superior bladder wall, which was repaired in one layer in a continuous-locking fashion. The patient was discharged home on the fifth postoperative day having an uneventful recovery. Conclusion: Bladder rupture frequently presents with non-specific clinical signs; as a result, it is easily misdiagnosed, especially when it occurs with a non-typical mechanism of injury. Pseudorenal failure is a relatively obscure entity that may help the clinician suspect a bladder perforation. Laparoscopic repair with a single-layer continuous suture technique is a safe and feasible treatment in hemodynamically stable patients. Prospective research is required to specify the optimal timing of catheter removal after bladder repair.
Regulation of Ras-GTPase Signaling and Localization by Post-Translational Modifications
Authors: Nair Arathi , Saha Bhaskar
Published: April 2023

Ras, a GTP-GDP binary switch protein, transduces signals from diverse receptors to regulate various signaling networks. Three Ras genes encode for protein isoforms, namely, Harvey Ras (H-Ras), Kirsten Ras (K-Ras, with two splice variants, K-Ras4A and K-Ras4B), and Neuroblastoma Ras (N-Ras). The isoforms undergo a series of post-translational modifications that enable their membrane attachment and biological activity. The activation of Ras isoforms is tightly regulated, and any dysregulation affects cellular processes, such as cell division, apoptosis, differentiation, cell migration, etc. The Ras gene is highly prone to mutation, and ~30% of cancers carry somatic mutations in Ras, whereas germline mutations clinically manifest as various rasopathies. In addition to regulation by the Guanine nucleotide exchange factors and the GTPase activation proteins, Ras signaling, and localization are also regulated by phosphorylation-dephosphorylation, ubiquitination, nitrosylation, and acetylation. Herein, we review the regulation of Ras signaling and localization by various regulatory enzymes in depth and assess the current status of Ras drug discovery targeting these regulatory enzymes.
How Pacifika Arts Reveal Interconnected Losses for People and Place in a Changing Climate
Authors: Clissold Rachel , Furlong Ellie , McNamara Karen E. , Westoby Ross , Latai-Niusulu Anita
Published: April 2023

The loss and damage transpiring because of anthropogenic climate change is a confronting reality, especially for frontline communities of the Pacific Islands. Understandings and assessments of loss and damage often fall short on coverage of intangible and noneconomic dimensions, such as losses to culture, place, Indigenous knowledge, and biodiversity, among others. In responding to this knowledge deficit, this paper turns its attention to the burgeoning Pacifika arts community because creative and cultural expressions have been critical avenues for sharing experiences, navigating loss, and exploring grief throughout history, including in the context of climate-driven loss. We analyse a series of Pacifika spoken, written, and visual items (n = 44), including visual art, poetry, song, film, documentary, and theatre, to identify the key categories and themes of noneconomic loss and damage (NELD) that emerge, better understand their nature, indicate their levels of prominence, reflect on them in relation to existing NELD frameworks and categories, and identify strategies for processing and coping. Our findings add to existing understandings of losses to territory, cultural heritage, human mobility, and health while also putting forward identity and agency as additional prominent NELD types. We emphasise that loss occurs within an interconnected and complex system that is centred on the critical relationships between people and their land, and greater attention must be paid to this interconnectivity as the foundation of identity and wellbeing. These perspectives enable stakeholders to better integrate experiences of NELD into future planning efforts so that they are not skewed (i.e., considering only economic loss and damage) or discounting people’s experiences. This will be critical for holistically building greater resilience and for communication in international fora and climate negotiations.
Bioeconomy Innovation Networks in Urban Regions: The Case of Stuttgart
Authors: Stöber Lea F. , Boesino Marius , Pyka Andreas , Schuenemann Franziska
Published: April 2023

For a successful transformation towards a sustainable bioeconomy, cooperative knowledge creation leading to innovations through research at the company and academic level are important. Urban regions are the centre of economic and research activities. The example of the region of Stuttgart, which aims to complement its mature industrial structure with new opportunities related to the knowledge-based bioeconomy, is an interesting case for the application of social network analysis to shed light on the dynamics of innovation networks to support the transformation of urban regions. As with smaller spatial levels of observation connectivity in network decreases, we find a scale-free network structure for the supra-regional network and a star-like network structure for the regional network, with two universities and one transfer-oriented research institutes at the core. While research collaborations beyond regional borders and across different industries foster knowledge co-creation, the central actors can be recognized as gatekeepers who dominantly influence knowledge flows. To potentially strengthen the resilience of the network, policy and industry associations serving as network facilitators can foster collaboration between periphery actors. The case of the Stuttgart region impressively illustrates the opportunities of the knowledge-based bioeconomy for urban regions and the complementary role traditional manufacturing sectors can take in the transformation towards higher degrees of sustainability.
The Rendering of Multilingual Occurrences in Netflix’s Italian Dub Streams: Evolving Trends and Norms on Streaming Platforms
Authors: Savoldelli Sofia , Spiteri Miggiani Giselle
Published: April 2023

Given the vast scholarly attention paid to multilingualism on traditional media over the years, it seems timely to focus on streaming platforms. This paper sets out to identify potential norms for the rendering of multilingual occurrences in the localised content of Netflix series. It also seeks to explore whether streaming translation practices related to multilingualism differ from the consolidated norms and practices for TV and cinema content. The chosen data sample consists of the Italian dub streams of five TV Netflix-produced shows featuring multilingualism as a main characteristic. The strategies and techniques adopted in each series are singled out, quantified, and labelled according to a combination of taxonomies. These include dubbing, revoicing, subtitling, part-subtitling, diegetic interpreting, unchanged speech transfer, and no translation. A wider analysis is also carried out across all the data sample to draw patterns on a macro level. The findings reveal a strong tendency to mark and preserve multilingualism, in line with Netflix’s own policies and dubbing specifications. Transfer unchanged combined with subtitles emerges as the most recurrent strategy, while the dub-over strategy accounts for 13% of the multilingual occurrences in the data sample. Extensive neutralisation is therefore not encountered. That said, a certain degree of overlap between multilingual translation norms on Netflix and conventional Italian dubbing practices (which tend to neutralise) can still be observed.
The Universally Conserved Unconventional G Protein YchF Is Critical for Growth and Stress Response
Authors: Lin Zhaoheng , Li Rongfang , Han Zhiwei , Liu Yi , Gao Liyang , Huang Suchang , Miao Ying , Miao Rui
Published: April 2023

The ancient guanine nucleotide-binding (G) proteins are a group of critical regulatory and signal transduction proteins, widely involved in diverse cellular processes of all kingdoms of life. YchF is a kind of universally conserved novel unconventional G protein that appears to be crucial for growth and stress response in eukaryotes and bacteria. YchF is able to bind and hydrolyze both adenine nucleoside triphosphate (ATP) and guanosine nucleoside triphosphate (GTP), unlike other members of the P-loop GTPases. Hence, it can transduce signals and mediate multiple biological functions by using either ATP or GTP. YchF is not only a nucleotide-dependent translational factor associated with the ribosomal particles and proteasomal subunits, potentially bridging protein biosynthesis and degradation, but also sensitive to reactive oxygen species (ROS), probably recruiting many partner proteins in response to environmental stress. In this review, we summarize the latest insights into how YchF is associated with protein translation and ubiquitin-dependent protein degradation to regulate growth and maintain proteostasis under stress conditions.
Correlation between Maspin Levels in Different Biological Samples and Pathologic Features in Colorectal Adenocarcinomas
Authors: Bratei Alexandru Adrian , Stefan-van Staden Raluca-Ioana
Published: April 2023

Maspin is an important biomarker which was proven to be correlated to many pathological features that can help the oncologists, the surgeons and also the pathologists for choosing the personalized treatment of the patients. Maspin expression correlates with the budding of colorectal adenocarcinomas that is usually used mostly in immunohistochemistry. In this preliminary study, a small number of patients with clinical and pathological features were selected. Four kinds of samples (tumoral tissues, blood, saliva and urine) were analyzed using a stochastic method using stochastic microsensors. Whole blood maspin concentration values were related to budding, molecular subtype and location. Tissular maspin concentrations were related to location, maxi-mum diameter and pN value from TNM staging system. Salivary maspin concentrations were related to budding, mucinous compound and macroscopic features. Urinary maspin concentrations were related to pT value from TNM staging system, budding and molecular subtype. The correlations made in this paper may be used for fast diagnostic of colorectal adenocarcinomas, after which, it will be tested on a significant number of patients confirmed with colon cancer, in different stages of evolution.
Sonochemical Synthesis of CuO Nanoplatelets and Their Tribological Properties as an Additive in Synthetic Oil Using Reciprocating Tribometer
Authors: Azam Siraj , Park Sang-Shin
Published: April 2023

This Research aimed to improve the tribological properties of commercially available lubricating oil (5W-40) by incorporating CuO nanoplatelets (NPs) synthesized using a simple and cost-effective sonochemical method. To evaluate the performance of the nanolubricant, a reciprocating tribometer was indigenously designed and developed to measure the coefficient of friction (COF) and wear tracks between two AISI 1045 steel surfaces. The CuO NPs were characterized using XRD to confirm their purity and phase, while SEM and FE-TEM were utilized to study their morphology and composition. Raman spectroscopy was used to reveal three distinct Raman active peaks of CuO at 283, 330, and 616 cm−1. Zeta potential measurements demonstrated good dispersion quality, with a value of 92.0 mV for 0.1% concentration. SEM and FE-TEM analysis of the nanolubricant showed the formation of a tribo-film over the CuO NPs and adding 0.1% CuO NPs reduced COF by 32%. These findings suggest that incorporating synthesized CuO NPs in commercially available lubricating oil can enhance its tribological properties, leading to improved machine efficiency and lifespan, as well as reduced energy demand. Overall, the study demonstrates the potential benefits of using CuO nanoplatelets as an additive in lubricating oil, which could have significant implications for the development of more efficient nanolubricants.
2-(2,5-Dimethoxyphenyl)pyrrole-3-carbonitrile
Authors: Grimm Yannic , Schollmeyer Dieter , Detert Heiner
Published: April 2023

The Cadogan reaction of 2-aryl-3-nitropyridines leads to δ-carbolines. The title compound is a side product in this reaction, generated via a ring opening of a nitrene and cyclization. A crystal structure analysis gives an enormous unit cell.
Effect of Material Change on Stirnol Engine: A Combination of NiTiNOL (Shape Memory Alloy) and Gamma Stirling Engine
Authors: Arif Humayun , Shah Aqueel , Ratlamwala Tahir Abdul Hussain , Kamal Khurram , Khan Maqsood Ahmed
Published: April 2023

Population explosion, industrialization, and urbanization have accelerated energy requirements across the globe. This has led to the human quest to find simple and cost-effective energy solutions. A promising solution is the revival of the Stirling engine with the addition of Shape Memory Alloy NiTiNOL in it. The experimental results reveal that the addition of a NiTiNOL spring at the base plate of the Stirling engine enhances the overall efficiency of the engine, demonstrating some impact of the shape memory alloy toward the performance output of the Stirling engine. The newly modified engine has been named the STIRNOL ENGINE. The comparative study of Stirling and Stirnol engines reveals a minuscule efficiency improvement, yet there is a furtherance that opens a window for future researchers to get a lead and venture into this new field. We are confident that with more complex designs and better Stirling and NiTiNOL combinations, more efficient engines can be invented in the future. This research focuses on changing the material of the base plate of the Stirnol engine and ascertaining its performance differential through the integration of the NiTiNOL spring. A minimum of four types of materials are utilized for experimentation.
Comparison of Implant Surgery Methods of Cortical Tapping and Cortical Widening in Bone of Various Density: A Three-Dimensional Finite Element Study
Authors: Baek Yeon-Wha , Lim Young-Jun , Kim Bongju
Published: April 2023

Purpose: The primary stability of a dental implant is critical for successful osseointegration during immediate loading. The cortical bone should be prepared to achieve enough primary stability, but not overcompressed. In this study, we investigated the stress and strain distribution in the bone around the implant induced by the occlusal force applied during immediate loading at various bone densities by the FEA method to compare cortical tapping and widening surgical techniques. Materials and Methods: A three-dimensional geometrical model of a dental implant and bone system was created. Five types of bone density combination (D111, D144, D414, D441 and D444) were designed. Two surgical methods—cortical tapping and cortical widening—were simulated in the model of the implant and bone. An axial load of 100 N and an oblique load of 30 N were applied to the crown. The maximal principal stress and strain were measured for comparative analysis of the two surgical methods. Results: Cortical tapping showed lower maximal stress of bone and maximal strain of bone than cortical widening when dense bone was located around the platform, regardless of the direction of the applied load. Conclusions: Within the limitations of this FEA study, it can be concluded that cortical tapping is biomechanically more advantageous to the implants under occlusal force during immediate loading, especially when the bone density around the platform is high.
Cyclic Voltammetry of Screen-Printed Carbon Electrode Coated with Ag-ZnO Nanoparticles in Chitosan Matrix
Authors: Herbei Elena Emanuela , Alexandru Petrică , Busila Mariana
Published: April 2023

In this paper, the authors describe the fabrication of nanocomposite chitosan-based systems of zinc oxide (ZnO), silver (Ag) and Ag-ZnO. Recently, the development of coated screen-printed electrodes using metal and metal oxide nanoparticles (NPs) for the specific detection and monitoring of different cancer tumors has been obtaining important results. Ag, ZnO NPs and Ag-ZnO prepared by the hydrolysis of zinc acetate blended with a chitosan (CS) matrix were used for the surface modification of screen-printed carbon electrodes (SPCEs) in order to analyze the electrochemical behavior of the typical redox system of a 10 mM potassium ferrocyanide—0.1 M buffer solution (BS). The solutions of CS, ZnO/CS, Ag/CS and Ag-ZnO/CS were prepared in order to modify the carbon electrode surface, and were measured at different scan rates from 0.02 V/s to 0.7 V/s by cyclic voltammetry. The cyclic voltammetry (CV) was performed on a house-built potentiostat (HBP). The cyclic voltammetry of the measured electrodes showed the influence of varying the scan rate. The variation of the scan rate has an influence on the intensity of the anodic and cathodic peak. Both values of currents (anodic and cathodic currents) have higher values for 0.1 V/s (Ia = 22 μA and Ic = −25 μA) compared to the values for 0.06 V/s (Ia = 10 μA and Ic = −14 μA). The CS, ZnO/CS, Ag/CS and Ag-ZnO/CS solutions were characterized using a field emission scanning electron microscopy (FE-SEM) with EDX elemental analysis. The modified coated surfaces of screen-printed electrodes were analyzed using optical microscopy (OM). The present coated carbon electrodes showed a different waveform compared to the voltage applied to the working electrode, depending on the scan rate and chemical composition of the modified electrodes.
Obtaining of Mg-Zn Co-Doped GaN Powders via Nitridation of the Ga-Mg-Zn Metallic Solution and Their Structural and Optical Properties
Authors: Gastellóu Erick , García Rafael , Herrera Ana M. , Ramos Antonio , García Godofredo , Hirata Gustavo A. , Luna José A. , Carrillo Roberto C. , Rodríguez Jorge A. , Robles Mario , Ramírez Yani D. , Martínez Guillermo
Published: April 2023

Mg-Zn co-dopedGaN powders via the nitridation of a Ga-Mg-Zn metallic solution at 1000 °C for 2 h in ammonia flow were obtained. XRD patterns for the Mg-Zn co-dopedGaN powders showed a crystal size average of 46.88 nm. Scanning electron microscopy micrographs had an irregular shape, with a ribbon-like structure and a length of 8.63 µm. Energy-dispersive spectroscopy showed the incorporation of Zn (Lα 1.012 eV) and Mg (Kα 1.253 eV), while XPS measurements showed the elemental contributions of magnesium and zinc as co-dopant elements quantified in 49.31 eV and 1019.49 eV, respectively. The photoluminescence spectrum showed a fundamental emission located at 3.40 eV(364.70 nm), which was related to band-to-band transition, besides a second emission found in a range from 2.80 eV to 2.90 eV (442.85–427.58 nm), which was related to a characteristic of Mg-doped GaN and Zn-doped GaN powders. Furthermore, Raman scattering demonstrated a shoulder at 648.05 cm−1, which could indicate the incorporation of the Mg and Zn co-dopants atoms into the GaN structure. It is expected that one of the main applications of Mg-Zn co-doped GaN powders is in obtaining thin films for SARS-CoV-2 biosensors.
Working Condition Identification Method of Wind Turbine Drivetrain
Authors: Huang Yuhao , Chen Huanguo , Dai Juchuan , Tao Hanyu , Wang Xutao
Published: April 2023

The operation state of the wind turbine drivetrain is complex and variable, making it difficult to accurately evaluate under the drivetrain’s anomalies. In order to accurately identify the operating state of the main drivetrain, a method for working condition identification is proposed. Firstly, appropriate working condition identification parameters are selected and distinguished from the working condition feature parameters. Secondly, the aerodynamic power prediction model is established, which solves the problem of inaccurate theoretical estimation. Finally, after the historical working conditions are classified, the working condition identification model is established, and the proposed method is analyzed and validated by cases. The results show that the method can accurately identify the working conditions, avoiding the influence of an abnormal state of drivetrain, and provide a basis for real-time state monitoring and evaluation.
Hybrid Method for Inverse Elastic Obstacle Scattering Problems
Authors: Yin Yuhan , Liu Juan
Published: April 2023

The problem of determining the shape of an object from knowledge of the far-field of a single incident wave in two-dimensional elasticity was considered. We applied an iterative hybrid method to tackle this problem. An advantage of this method is that it does not need a forward solver, and therefore, the exact boundary condition is not essential. By deriving the Fréchet derivatives of two boundary operators, we established reconstruction algorithms for objects with Dirichlet, Neumann, and Robin boundary conditions; by introducing a general boundary condition, we also established the reconstruction algorithm for objects with unknown physical properties. Numerical experiments showed the effectiveness of the proposed method.
Generalized Halanay Inequalities and Relative Application to Time-Delay Dynamical Systems
Authors: Wang Chunsheng , Liu Xiangdong , Jiao Feng , Mai Hong , Chen Han , Lin Runpeng
Published: April 2023

A class of generalized Halanay inequalities is studied via the Banach fixed point method and comparison principle. The conditions to ensure the boundedness and stability of the zero solution are obtained in this study. This research provides a new approach to the study of the boundedness and stability of Halanay inequality. Numerical examples and simulation results verify the validity and superiority of the conclusions obtained in this study.
The Regulator Problem to the Convection–Diffusion Equation
Authors: Ramírez Andrés A. , Jurado Francisco
Published: April 2023

In this paper, from linear operator, semigroup and Sturm–Liouville problem theories, an abstract system model for the convection–diffusion (C–D) equation is proposed. The state operator for this abstract system model is here defined as given in the form of the Sturm–Liouville differential operator (SLDO) plus an integral term of the same SLDO. Our aim is to achieve the trajectory tracking task in the presence of external disturbances to the C–D equation invoking the regulator problem theory, where the state from a finite-dimensional exosystem is the state to the feedback law. In this context, the regulator (Francis) equations, established from the abstract system model for the C–D equation, here are solved; i.e., the state feedback regulator problem (SFRP) for the C–D system has a solution. Our proposal is validated via numerical simulation results.
Parametric Study on the Sensitivity of Different Properties of Waves Propagating through an Incompressible Medium
Authors: Aamir Muhammad , Alhejaili Weaam , Lotfy Khalid , El-Bary Alaa , Jahangir Adnan
Published: April 2023

This article deals with the study of disturbance that travels through the transversely isotropic medium in the form of waves. The particles of the considered medium have an additional property of small-scale internal rotation along with macroscopic translational deformation. This extra translational freedom causes the medium to be micropolar in nature. Along with this, the medium is incompressible, and the dispersion relation of waves propagating through the medium is obtained under specific plan-strain conditions. From the dispersion relation, we can conclude that because of incompressibility, three transverse waves propagate through the medium. The velocity profile, attenuation coefficient, and specific heat loss for these waves are discussed for a particular medium. Later, the special normalized local sensitivity analysis (NLSA) technique is used to depict the effects of parameters on the outcomes of the mathematical model. The obtained results are represented graphically for a particular medium. The proposed model is used to model the mechanical behavior of complex materials with microstructural heterogeneity, such as composites and biological tissues.
Finite-Element Method for the Simulation of Lipid Vesicle/Fluid Interactions in a Quasi–Newtonian Fluid Flow
Authors: Laadhari Aymen
Published: April 2023

We present a computational framework for modeling an inextensible single vesicle driven by the Helfrich force in an incompressible, non-Newtonian extracellular Carreau fluid. The vesicle membrane is captured with a level set strategy. The local inextensibility constraint is relaxed by introducing a penalty which allows computational savings and facilitates implementation. A high-order Galerkin finite element approximation allows accurate calculations of the membrane force with high-order derivatives. The time discretization is based on the double composition of the one-step backward Euler scheme, while the time step size is flexibly controlled using a time integration error estimation. Numerical examples are presented with particular attention paid to the validation and assessment of the model’s relevance in terms of physiological significance. Optimal convergence rates of the time discretization are obtained.
Parameter-Independent Event-Triggered Implicit UKF for the Celestial Navigation Using Time Delay Measurement
Authors: Gui Mingzhen , Wei Caisheng , Wei Yifeng , Xiong Kai , Zhang Chengxi , Dai Mingzhe
Published: April 2023

Celestial navigation using time delay measurement is an innovative autonomous navigation method. To calculate the equivalent measurement, the numerical method needs to be applied, which is time-consuming. The event-triggered mechanism intermittently and aperiodically processes measurements by judging if the update error has changed drastically. However, its performance is greatly affected by the constant threshold. To solve this problem, a parameter-independent event-triggered implicit unscented Kalman filter (UKF) is proposed and applied to the celestial navigation using time delay measurement. The innovation at the current moment and the updated estimate covariance at the last moment are compared with the previous value instead of the constant threshold. The event is automatically triggered when the accuracy of the state estimate is low. Simulation results indicate that the proposed parameter-independent event-triggered implicit UKF can reduce the running time by reducing unnecessary measurement updates, whose performance will not be affected by any parameter or window size. In a word, the proposed method substitutes the dynamic threshold for the constant threshold, ensuring that its performance will not be affected by any parameter or window size.
A Comprehensive Review of the Hermite–Hadamard Inequality Pertaining to Fractional Integral Operators
Authors: Tariq Muhammad , Ntouyas Sotiris K. , Shaikh Asif Ali
Published: April 2023

In the frame of fractional calculus, the term convexity is primarily utilized to address several challenges in both pure and applied research. The main focus and objective of this review paper is to present Hermite–Hadamard (H-H)-type inequalities involving a variety of classes of convexities pertaining to fractional integral operators. Included in the various classes of convexities are classical convex functions, m-convex functions, r-convex functions, (α,m)-convex functions, (α,m)-geometrically convex functions, harmonically convex functions, harmonically symmetric functions, harmonically (θ,m)-convex functions, m-harmonic harmonically convex functions, (s,r)-convex functions, arithmetic–geometric convex functions, logarithmically convex functions, (α,m)-logarithmically convex functions, geometric–arithmetically s-convex functions, s-convex functions, Godunova–Levin-convex functions, differentiable ϕ-convex functions, MT-convex functions, (s,m)-convex functions, p-convex functions, h-convex functions, σ-convex functions, exponential-convex functions, exponential-type convex functions, refined exponential-type convex functions, n-polynomial convex functions, σ,s-convex functions, modified (p,h)-convex functions, co-ordinated-convex functions, relative-convex functions, quasi-convex functions, (α,h−m)−p-convex functions, and preinvex functions. Included in the fractional integral operators are Riemann–Liouville (R-L) fractional integral, Katugampola fractional integral, k-R-L fractional integral, (k,s)-R-L fractional integral, Caputo-Fabrizio (C-F) fractional integral, R-L fractional integrals of a function with respect to another function, Hadamard fractional integral, and Raina fractional integral operator.
Seaweeds Arising from Brauer Configuration Algebras
Authors: Cañadas Agustín Moreno , Mendez Odette M.
Published: April 2023

Seaweeds or seaweed Lie algebras are subalgebras of the full-matrix algebra Mat(n) introduced by Dergachev and Kirillov to give an example of algebras for which it is possible to compute the Dixmier index via combinatorial methods. It is worth noting that finding such an index for general Lie algebras is a cumbersome problem. On the other hand, Brauer configuration algebras are multiserial and symmetric algebras whose representation theory can be described using combinatorial data. It is worth pointing out that the set of integer partitions and compositions of a fixed positive integer give rise to Brauer configuration algebras. However, giving a closed formula for the dimension of these kinds of algebras or their centers for all positive integer is also a tricky problem. This paper gives formulas for the dimension of Brauer configuration algebras (and their centers) induced by some restricted compositions. It is also proven that some of these algebras allow defining seaweeds of Dixmier index one.
Optimization of LFP Pouch Cell Tab Design for Uniform Temperature Distribution
Authors: Lee Jun , Chang Hyukkyun , Kim Chang-Wan
Published: April 2023

An increase in the size of large-format Li-ion batteries (LIBs) may lead to nonuniform temperature distribution, which degrades the performance and lifespan of the LIBs. To address this issue, we performed design optimization using a 3D electrochemical-thermal coupled model for 55 Ah LFP/graphite large-format pouch cells. To minimize temperature differences in Normal Tab (NT), Lateral Tab (LT), and Counter Tab (CT) types of LIBs, design optimization was performed on the width, height, and attachment position of each positive and negative Table The upper and lower limits of each design variable were set as constraints without exceeding the sum of the total area of the tabs of the initial NT type. Owing to the optimization of the NT, LT, and CT types, the temperature difference in the optimized CT type was 79.2% less than in the initial NT type. Additionally, the potential difference decreased by 37.1%, minimizing ohmic heat. Aging analysis of 2500 cycles was performed to analyze the improvement in the lifespan due to the uniform temperature distribution. Consequently, the capacity retention rate of the optimized CT type was 6.5% higher than that of the initial NT type. Thus, the temperature distribution and lifespan of LIBs were improved by design optimization.
Trends in Antibody Titers after SARS-CoV-2 Vaccination—Insights from Self-Paid Tests at a General Internal Medicine Clinic
Authors: Kusunoki Hiroshi , Ekawa Kazumi , Ekawa Masakazu , Kato Nozomi , Yamasaki Keita , Motone Masaharu , Shimizu Hideo
Published: April 2023

Background: The rise in antibody titers against the novel coronavirus (SARS-CoV-2) and its duration are considered an important indicator for confirming the effect of a COVID-19 vaccine, and self-paid tests of antibody titer are conducted in many facilities nationwide. Methods: The relationship between the number of days after the second and third dose of vaccines, age, and antibody titer was determined from the medical records of general internal medicine clinics that conducted self-paid testing of the SARS-CoV-2 antibody titer using Elecsys Anti-SARS-CoV-2 S (Roche Diagnostics); the relationship between the number of days after two or more doses of vaccines and antibody titer was also determined. We also examined the antibody titers in cases of spontaneous infection with SARS-CoV-2 after two or more doses of the vaccine. Results: Log-transformed SARS-CoV-2 antibody titers measured within 1 month from the second or third dose of vaccine showed a negative correlation with age (p < 0.05). In addition, the log-transformed antibody titers also showed a negative correlation trend with the number of days after the second dose of vaccine (p = 0.055); however, there were no significant correlations between the log-transformed antibody titers and the number of days after the third dose of vaccine. The median antibody titer after the third vaccination was 18,300 U/mL, more than 10 times the median antibody titer after the second dose of vaccine, of 1185 U/mL. There were also some cases of infection after the third or fourth dose of vaccine, with antibody titers in the tens of thousands of U/ml after infection, but the patients still received further booster vaccinations after the infection. Conclusions: The antibody titers after the third vaccination did not attenuate after a short follow-up period of one month, while they tended to attenuate after the second vaccination. It is considered that many people in Japan received further booster vaccinations after spontaneous infection, even though they already had antibody titers in the tens of thousands of U/mL due to “hybrid immunity” after spontaneous infection following two or more doses of vaccine. The clinical significance of the booster vaccination in this population still needs to be thoroughly investigated and should be prioritized for those with low SARS-CoV-2 antibody titers.
Quercetin-Induced Enhancement of Nasal Epithelial Cells’ Ability to Produce Clara Cell 10-kD Protein In Vitro and In Vivo
Authors: Otaki Amane , Furuta Atsuko , Asano Kazuhito
Published: April 2023

Background: Quercetin, a polyphenolic flavonoid found in various plants and foods, is known to have antioxidant, antiviral and anticancer effects. Although quercetin is well known to exert anti-inflammatory and anti-allergic effects, the precise mechanisms by which quercetin favorably modifies the clinical status of allergic diseases, such as allergic rhinitis (AR), remain unclear. The present study examined whether quercetin could modulate the production of the endogenous anti-inflammatory molecule, Clara cell 10-kD protein (CC10), in vitro and in vivo. Methods: Human nasal epithelial cells (1 × 105 cells/mL) were stimulated with 20 ng/mL of tumor necrosis factor-alpha (TNF) in the presence of quercetin for 24 h. CC10 levels in culture supernatants were examined by ELISA. Sprague Dawley rats were sensitised with toluene 2,4-diisocyanate (TDI) by intranasal instillation of 10% TDI in ethyl acetate at a volume of 5.0 μL once daily for five days. This sensitisation procedure was repeated after an interval of two days. The rats were treated with different dosages of quercetin once daily for five days starting on the 5th day following the second sensitization. Nasal allergy-like symptoms induced by the bilateral application of 5.0 μL of 10% TDI were assessed by counting sneezing and nasal-rubbing behaviours for 10 min immediately after the TDI nasal challenge. The levels of CC10 in nasal lavage fluids obtained 6 h after TDI nasal challenge were examined using ELISA. Results: The treatment of cells with low doses of quercetin (<2.5 μM) scarcely affected TNF-induced CC10 production from nasal epithelial cells. However, the ability of nasal epithelial cells to produce CC10 after TNF stimulation significantly increased on treatment with quercetin doses (>5.0 μM). The oral administration of quercetin (>25 mg/kg) for five days significantly increased the CC10 content in nasal lavage fluids and attenuated the nasal symptoms induced by the TDI nasal challenge. Conclusions: Quercetin inhibits AR development by increasing the ability of nasal epithelial cells to produce CC10.
Microstructure and Mechanical Properties of Al-4Mg-0.3Cu Alloy after HPT and Postdeformation Annealing
Authors: Yang Xiaohui , Gao Chao , Ku Tingting , Yang Wenlu , Guo Yanping , Wang Linzeng , Chen Huiqin , Li Xiaofeng , Hu Rujie , Cui Jianyu
Published: April 2023

The effects of different strain levels applied through high-pressure torsion (HPT) deformation following annealing on the microstructural evolution, thermal stability, and mechanical properties of Al-4Mg-0.3Cu alloy were investigated. The results reveal that Cu-segregated grain boundaries (GBs) were generated in the ultrafine-grained Al-4Mg-0.3Cu alloy with high angle grain boundaries. By contrast, the phenomenon of Cu segregation was not found in micron-scale and submicron-scale grains with low-angle grain boundaries. The mechanism of Cu segregation in ultrafine-grained Al-4Mg-0.3Cu alloy was discussed. After heat treatment, Cu segregation induced the precipitation of the dense Al2CuMg phase at GBs, which strongly inhibit grain growth and improve thermal stability. Stress–strain curves of as-cast, 5-turn, and 10-turn HPT samples showed that fracture strength significantly increased, attributed to grain size refinement, dislocation density increase, and Cu segregation at GBs. After heat treatment, 5-turn and 10-turn HPT samples demonstrate an enhanced elongation to fracture with a slight reduction of fracture strength.
Preparation of High-Purity Magnesium from Electrolytically Produced Crude Magnesium via Vacuum Distillation
Authors: Ma Zhanshan , Ma Shangrun , Zhu Fuxing , Li Kaihua , Sheng Zhuo , Li Zhanjun , Wang Yaowu
Published: April 2023

Metallic Mg is an important strategic metal and its properties are greatly affected by impurities. Silicothermic reduction and electrolysis are the most used approaches to prepare metallic Mg. The products of these processes need to be further refined to obtain high-purity Mg metal. However, previous research has mainly focused on refining the crude Mg (CM) produced via silicothermic reduction, whereas no in-depth investigations have been conducted on refining the CM produced via electrolysis. Here, vacuum distillation was used to refine electrolytically produced CM. The content and morphological characteristics of the impurity elements in CM were studied via glow discharge mass spectrometry, mineral dissociation analysis, and electron probe microanalysis. The effect of different distillation temperatures and times on the quality of the refined Mg was investigated. The results show that the main impurity elements are Al, Fe, Si, Ti, Cr, S, Cl, and Ni. The content of impurities, such as Si, Al, Fe, Ni, Ti, and Cr, in the refined Mg is significantly reduced at a temperature of 1023 K and a time of 120 min, and the purity of the refined Mg reaches 99.99%, which meets the Mg9999 national standard for primary Mg ingots in China (GB/3499-2011).