bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of the Electrodialysis Performance in Tartrate Stabilization of a Red Wine Using Aliphatic and Aromatic Commercial and Modified Ion-Exchange Membranes
Authors: Pasechnaya Evgeniia , Tsygurina Kseniia , Ponomar Maria , Chuprynina Daria , Nikonenko Victor , Pismenskaya Natalia
Published: January 2023

The application of electrodialysis for tartrate stabilization and reagent-free acidity correction of wine and juices is attracting increasing interest. New aliphatic membranes CJMC-3 and CJMA-3 and aromatic membranes CSE and ASE were tested to determine their suitability for use in these electrodialysis processes and to evaluate the fouling of these membranes by wine components for a short (6–8 h) operating time. Using IR spectroscopy, optical indication and measurement of surface contact angles, the chemical composition of the studied membranes, as well as some details about their fouling by wine components, was clarified. The current–voltage charsacteristics, conductivity and water-splitting capacity of the membranes before and after electrodialysis were analyzed. We found that in the case of cation-exchange membranes, complexes of anthocyanins with metal ions penetrate into the bulk (CJMC-3) or are localized on the surface (CSE), depending on the degree of crosslinking of the polymer matrix. Adsorption of wine components by the surface of anion-exchange membranes CJMA-3 and ASE causes an increase in water splitting. Despite fouling under identical conditions of electrodialysis, membrane pair CJMC-3 and CJMA-3 provided 18 ± 1 tartrate recovery with 31 · 10−3 energy consumption, whereas CSE and ASE provided 20 ± 1% tartrate recovery with an energy consumption of 28 · 10−3 Wh, in addition to reducing the conductivity of wine by 20 ± 1%. The casting of aliphatic polyelectrolyte films on the surface of aromatic membranes reduces fouling with a relatively small increase in energy consumption and approximately the same degree of tartrate recovery compared to pristine CSE and ASE.
Effect of High-Sintering-Temperature Reduction Behavior on Coke Solution Loss Reaction with Different Thermal Properties
Authors: Jin Jin , Wang Qi , Zhang Song
Published: January 2023

With the shortage of high-quality coking coal resources and the pursuit of low-cost smelting, the types and sources of coal have changed. Therefore, it is difficult to establish an effective correlation between the existing evaluation indexes of coke thermal performance and the production indexes of the blast furnace. The dissolution deterioration of coke directly affects the production benefits of the blast furnace, and the dissolution deterioration of blast furnace coke is the result of ore–coke coupling. To better understand the mechanism of the coupling reaction relative to the thermal properties of coke, this paper experimentally studies ore–coke coupling between two kinds of coke and one kind of blast furnace standing sinter which have different reactivities but are used in practical applications. This method adopts a matched thermogravimetric device. By analyzing and calculating the high-temperature reduction behavior and characteristics of the sinter and the dissolution loss behavior and characteristics of coke in the gas–solid coupling reaction test of coke and sinter, and comparing and fitting the coupling reaction factors of the coupling reaction and the thermal properties of coke, it was revealed that the real degradation behavior of coke was affected by the reduction reaction of the sinter. The results show that the temperature range with the best matching degree between the reduction reaction of oxygen supply from sinter and the gasification reaction of oxygen consumption from coke is at a position where the coupling factor is closest to 1. In the gas–solid coupling reaction between low-reactivity coke and sinter, the strongest dissolution rate, RCSL, is approximately 1200 °C, while in the gas–solid coupling reaction between high-reactivity coke and sinter, the RCSL is approximately 1100 °C. The minimum strength, CSCSL, of high-active coke and sinter after dissolution is approximately 1100 °C, while that of low-active coke and sinter after dissolution is approximately 1200 °C. It is shown that there is a good linear relationship between the RCSL of high- and low-reactive coke and strength after dissolution loss CSCSL.
Flow and Reaction Characteristic in a Stirred Reactor for Corrosion Process of Reduced Ilmenite
Authors: Zhao Qiuyue , Li Yang , Zheng Mingzhao , Gang Shuangfu , Zhang Ting-An
Published: January 2023

The technology for producing artificial rutile by the reduction-rusting method has the advantages of high product quality and less slag. A computational fluid dynamics (CFD) method is used to simulate the solid-liquid suspension and gas-liquid dispersion characteristics in the stirred corrosion reactor. The results show that the maximum volume fraction difference of the solid phase in the reactor with an upward-lifting impeller is 0.02, and for the downforce type, it is 0.04. When the stirring speed is increased from 100 rpm to 150 rpm, the radial dispersion area of the gas phase is increased; after the six-inclined-blade disk turbine is selected as the lower impeller, the proportion of bubbles with a diameter less than 0.004 m in the reactor is increased from 26% to 45%. The content of metallic iron in the product of the corrosion reaction is 1.67% in the stirred reactor with a six-inclined-blade disk turbine as the lower impeller.
Effect of Thermal Cycling on Grain Evolution and Micro-Segregation in Selective Laser Melting of FGH96 Superalloy
Authors: Li Lin , Liu Furong , Nie Shijin , Wang Qin , Zhao Rongxia , Zhang Yongzhi , Feng Haoyuan , Lin Xin
Published: January 2023

Extremely rapid heating and cooling rates during the additive manufacturing (AM) process generate complicated thermal cycles, which affect the microstructure evolution and ultimate mechanical properties of the alloy. In this paper, FGH96 blocks with a height of 6 mm were prepared by selective laser melting (SLM) and the microstructure was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Comparing specimens of varying heights, it was found that subsequent thermal cycles (STC) coarsened some solidified grains and accelerated the grain growth along the build direction, together with an increase in texture intensity and high-angle grain boundaries (HAGBs). After coarsening the grains in the middle portion of the built block, finer grains were observed near the top area due to a faster cooling rate. There were numerous dislocations in the grain because of the occurrence of unequal internal tension. In the middle of the sample with stable thermal cycles, the dislocations were both perpendicular to the grain growth direction and 45° off it. In spite of the texture characteristics, the segregation of elements was also found to be influenced by thermal cycling. Inherent reheating leads to the increase in the Laves phase and the decrease in the γ’ phase as subsequent deposition. This was also one of the reasons why the microhardness of the sample decreased as the building height and the other reason being the decrease in the solution treatment of the later sediments.
Finite Element Analysis of Restraint Intensities and Welding Residual Stresses in the Ti80 T-Joints
Authors: Zhang Liang , Wang Qi , Chen Pan , Luo Bingbing , Zhu Ziling , Yang Xiaoyu
Published: January 2023

The restraint intensity of Ti80 T-joints was investigated using finite element analyses. The influence of slit height, vertical plate thickness and base plate thickness was studied, respectively. Results show that the slit height and vertical plate thickness have a significant impact, while the effect of base plate thickness is negligible. A prediction model of restraint intensity was constructed through binary linear regression; the error was estimated at about 10%. Then, finite element simulations were carried out to study the welding residual stresses of specimens with different restraint intensities. The results show that residual stresses on the backing weld surface are higher in the middle and lower at both ends, while the weld root shows opposite results. In general, stresses at the weld root are greater than those on the weld surface. The mean value of the residual stress at the weld root increases with the increase in restraint intensity but not uniformly, i.e., it is slow at first and then it increases rapidly. A prediction model of the residual stress was produced through cubic fitting, and the errors between the finite element simulations and predictions were about 8%. Using the prediction model, the residual stress of actual Ti80 alloy workpieces can be estimated before welding, and a corresponding strategy for avoiding cracks can be generated.
Analysis on the Quenching Deformation Characteristics of Light Cast Aluminum Alloy Wheels and Their Control Strategies
Authors: Chi Hui , Yan Meng , Xu Zhengqi , Xiang Pengfei , An Zijun , Huang Huagui
Published: January 2023

The purpose of this paper is to develop a new technology for controlling the quenching deformation of light cast aluminum alloy wheels. First, based on the existing wheel heat treatment process, a gas–liquid–solid multi-phase flow coupling model was established through the ANSYS Workbench platform to analyze the gas–liquid phase change, heat exchange on wheel surface and quenching deformation characteristics during the process of wheel immersion into the water. The results show that heat exchange characteristics of the wheel surface are comprehensively affected by wheel structure, quenching fluid flow field and gas–liquid phase transition. There are a lot of non-uniform heat exchange areas in the outer rim, spoke area and center area, which affect the overall deformation characteristics. Affected by spoke structure, the maximum deformation occurs at the outer and inner rim end faces farthest away from the wheel. Based on the above research, this paper independently develops a new deformation control strategy of spray and water immersion composite step process. Through spraying, the influence of spoke structural stiffness on the overall deformation characteristics of the wheel is effectively reduced, and the wheel deformation control is realized by meeting the mechanical properties of the wheel, with the maximum deformation reduction of 39.2%. This study provides a new option for the integrated control of deformation and mechanical properties of aluminum alloy wheels.
Co-Infection of Potential Tick-Borne Pathogens of the Order Rickettsiales and Borrelia burgdorferi s. l. and Their Link to Season and Area in Germany
Authors: Hoffmann Angeline , Müller Thomas , Fingerle Volker , Silaghi Cornelia , Noll Matthias
Published: January 2023

The prevalence of potential human pathogenic members of the order Rickettsiales differs between Borrelia burgdorferi sensu lato-positive and -negative tick microbiomes. Here, co-infection of members of the order Rickettsiales, such as Rickettsia spp., Anaplasma phagocytophilum, Wolbachia pipientis, and Neoehrlichia mikurensis as well as B. burgdorferi s.l. in the tick microbiome was addressed. This study used conventional PCRs to investigate the diversity and prevalence of the before-mentioned bacteria in 760 nucleic acid extracts of I. ricinus ticks detached from humans, which were previously tested for B. burgdorferi s.l.. A gltA gene-based amplicon sequencing approach was performed to identify Rickettsia species. The prevalence of Rickettsia spp. (16.7%, n = 127) and W. pipientis (15.9%, n = 121) were similar, while A. phagocytophilum was found in 2.8% (n = 21) and N. mikurensis in 0.1% (n = 1) of all ticks. Co-infection of B. burgdorferi s. l. with Rickettsia spp. was most frequent. The gltA gene sequencing indicated that Rickettsia helvetica was the dominant Rickettsia species in tick microbiomes. Moreover, R, monacensis and R. raoultii were correlated with autumn and area south, respectively, and a negative B. burgdorferi s. l. finding. Almost every fifth tick carried DNA of at least two of the human pathogenic bacteria studied here.
Protocooperative Effect of Sphaerodes mycoparasitica Biocontrol and Crop Genotypes on FHB Mycotoxin Reduction in Bread and Durum Wheat Grains Intended for Human and Animal Consumption
Authors: Powell Antonia J. , Kim Seon Hwa , Cordero Jorge , Vujanovic Vladimir
Published: January 2023

The occurrence of Fusarium Head Blight (FHB) mycotoxins in wheat grains is a major threat to global food safety and security. Humans and animals are continuously being exposed to Fusarium mycotoxins such as deoxynivalenol (DON) and its acetylated derivatives 3ADON and 15ADON through the ingestion of contaminated food or grain-based diet. In this study, a host-specific mycoparasite biocontrol agent (BCA), Sphaerodes mycoparasitica, significantly reduced FHB mycotoxin occurrence in harvested wheat grains from Fusarium graminearum 3ADON chemotype infected plants in greenhouse. Four genotypes of wheat, two common wheat and two durum wheat cultivars with varying FHB resistance levels were used in this study. Principal Coordinate Analysis (PCoA) using Illumina ITS sequences depicted beta diversity changes in Fusarium species indicating that both plant cultivar and BCA treatments influenced the Fusarium species structure and mycotoxin occurrence in grains. Fusarium graminearum complex (cluster A), F. avenaceum and F. acuminatum (cluster B), and F. proliferatum (cluster C) variants were associated with different FHB mycotoxins based on LC-MS/MS analyses. The predominant FHB mycotoxins measured were DON and its acetylated derivatives 3ADON and 15ADON. The BCA reduced the occurrence of DON in grains of all four cultivars (common wheat: 1000–30,000 µg·kg−1.; durum wheat: 600–1000 µg·kg−1) to levels below the Limit of Quantification (LOQ) of 16 µg·kg−1. A relatively higher concentration of DON was detected in the two common wheat genotypes when compared to the durum wheat genotype; however, the percentage reduction in the wheat genotypes was greater, reaching up to 99% with some S. mycoparasitica treatments. Similarly, a higher reduction in DON was measured in susceptible genotypes than in resistant genotypes. This study’s findings underscore the potential of a Fusarium-specific S. mycoparasitica BCA as a safe and promising alternative that can be used in conjunction with other management practices to minimize FHB mycotoxins in cereal grain, food and feed intended for human and animal consumption.
KxCo1.5−0.5xFe(CN)6/rGO with Dual−Active Sodium Ion Storage Site as Superior Anode for Sodium Ion Battery
Authors: Zhou Gang , Fan Mincong , Wang Lei , Li Xianglin , Liu Danqing , Gao Feng
Published: January 2023

The unique and open large frame structures of prussian blue analogues (PBA) enables it for accommodating a large number of cations (Na+, K+, Ca2+, etc.), thus, PBA are considered as promising electrode materials for the rechargeable battery. However, due to the chemical composition, there are still many alkaline metal ions in the gap within the framework, which puts multivalent metals in PBA in a low valence state and affects the sodium storage performance. To improve the valence of metal ions in PBA materials, precursors prepared by co−precipitation method and hydrothermal method are used to synthesis KxCo1.5−0.5xFe(CN)6 through further chemical oxidation. Through the introducing of reduced graphene oxide (rGO) with excellent conductivity by a simple physical mixing method, the cycle stability and rate performance of the PBA material can be further improved. The K0.5Co1.2Fe(CN)6·2H2O/rGO anode prepared with 2 h hydrothermal time and further chemical oxidation, named as KCoHCP−H2−EK/rGO, exhibits a super electrochemical performance, delivering initial charge/discharge capacities of 846.7/1445.0 mAh·g−1, and a capacity retention of 58.2% after 100 cycles at a current density of 100 mA·g−1. The KCoHCP−H2−EK/rGO outstanding electrochemical behaviors are attributed to the unique dual−active site structure properties and the improved surface conductance of materials by rGO components.
Genomics of Tenacibaculum Species in British Columbia, Canada
Authors: Nowlan Joseph P. , Sies Ashton N. , Britney Scott R. , Cameron Andrew D. S. , Siah Ahmed , Lumsden John S. , Russell Spencer
Published: January 2023

Tenacibaculum is a genus of Gram-negative filamentous bacteria with a cosmopolitan distribution. The research describing Tenacibaculum genomes stems primarily from Norway and Chile due to their impacts on salmon aquaculture. Canadian salmon aquaculture also experiences mortality events related to the presence of Tenacibaculum spp., yet no Canadian Tenacibaculum genomes are publicly available. Ribosomal DNA sequencing of 16S and four species-specific 16S quantitative-PCR assays were used to select isolates cultured from Atlantic salmon with mouthrot in British Columbia (BC), Canada. Ten isolates representing four known and two unknown species of Tenacibaculum were selected for shotgun whole genome sequencing using the Oxford Nanopore’s MinION platform. The genome assemblies achieved closed circular chromosomes for seven isolates and long contigs for the remaining three isolates. Average nucleotide identity analysis identified T. ovolyticum, T. maritimum, T. dicentrarchi, two genomovars of T. finnmarkense, and two proposed novel species T. pacificus sp. nov. type strain 18-2881-AT and T. retecalamus sp. nov. type strain 18-3228-7BT. Annotation in most of the isolates predicted putative virulence and antimicrobial resistance genes, most-notably toxins (i.e., hemolysins), type-IX secretion systems, and oxytetracycline resistance. Comparative analysis with the T. maritimum type-strain predicted additional toxins and numerous C-terminal secretion proteins, including an M12B family metalloprotease in the T. maritimum isolates from BC. The genomic prediction of virulence-associated genes provides important targets for studies of mouthrot disease, and the annotation of the antimicrobial resistance genes provides targets for surveillance and diagnosis in veterinary medicine.
Molecular Targets for Foodborne Pathogenic Bacteria Detection
Authors: Paramithiotis Spiros
Published: January 2023

The detection of foodborne pathogenic bacteria currently relies on their ability to grow on chemically defined liquid and solid media, which is the essence of the classical microbiological approach. Such procedures are time-consuming and the quality of the result is affected by the selectivity of the media employed. Several alternative strategies based on the detection of molecular markers have been proposed. These markers may be cell constituents, may reside on the cell envelope or may be specific metabolites. Each marker provides specific advantages and, at the same time, suffers from specific limitations. The food matrix and chemical composition, as well as the accompanying microbiota, may also severely compromise detection. The aim of the present review article is to present and critically discuss all available information regarding the molecular targets that have been employed as markers for the detection of foodborne pathogens. Their strengths and limitations, as well as the proposed alleviation strategies, are presented, with particular emphasis on their applicability in real food systems and the challenges that are yet to be effectively addressed.
High-Quality Laser-Accelerated Ion Beams from Structured Targets
Authors: Matys Martin , Psikal Jan , Nishihara Katsunobu , Klimo Ondrej , Jirka Martin , Valenta Petr , Bulanov Sergei V.
Published: January 2023

In this work, we reviewed our results on the prospect of increasing the quality of ion acceleration driven by high-intensity laser pulses using low-Z structured targets. It is shown that the radiation pressure acceleration mechanism dominates over target normal sheath acceleration for assumed laser target parameters when the laser intensity is high enough. The target thickness is optimized for this regime and double-layer structure is investigated. When a corrugation is fabricated on the interface of such a target, a relativistic instability with Rayleigh–Taylor and Richtmyer–Meshkov like features can be driven by the target interaction with a high intensity laser pulse. The proper development of this instability leads to the generation of a collimated quasi-monoenergetic ion beam with lower emittance, divergence, and energy spread compared to a single and double-layer target with planar interface. A steep-front laser pulse is used in our simulations to mitigate other type of instabilities arising at the target surface from the laser–target interaction. We discuss the use of a plasma shutter to generate the required pulse profile, which also locally increases intensity. The obtained shape improves the ion acceleration, including higher maximal energy and lower beam divergence, in our simulation of a high-Z target.
Self-Attention Mechanism-Based Multi-Channel QoT Estimation in Optical Networks
Authors: Zhou Yuhang , Huo Xiaoli , Gu Zhiqun , Zhang Jiawei , Ding Yi , Gu Rentao , Ji Yuefeng
Published: January 2023

It is essential to estimate the quality of transmission (QoT) of lightpaths before their establishment for efficient planning and operation of optical networks. Due to the nonlinear effect of fibers, the deployed lightpaths influence the QoT of each other; thus, multi-channel QoT estimation is necessary, which provides complete QoT information for network optimization. Moreover, the different interfering channels have different effects on the channel under test. However, the existing artificial-neural-network-based multi-channel QoT estimators (ANN-QoT-E) neglect the different effects of the interfering channels in their input layer, which affects their estimation accuracy severely. In this paper, we propose a self-attention mechanism-based multi-channel QoT estimator (SA-QoT-E) to improve the estimation accuracy of the ANN-QoT-E. In the SA-QoT-E, the input features are designed as a sequence of feature vectors of channels that route the same path, and the self-attention mechanism dynamically assigns weights to the feature vectors of interfering channels according to their effects on the channel under test. Moreover, a hyperparameter search method is used to optimize the SA-QoT-E. The simulation results show that, compared with the ANN-QoT-E, our proposed SA-QoT-E achieves higher estimation accuracy, and can be directly applied to the network wavelength expansion scenarios without retraining.
Wavefront Reconstruction of Shack-Hartmann with Under-Sampling of Sub-Apertures
Authors: Huang Jian , Yao Lianqun , Wu Shuyun , Wang Gongchang
Published: January 2023

Shack-Hartmann wavefront sensor plays a key role in adaptive optics (AO) systems, which detect the aberrant wavefront by an array of micro-lenslets across the aperture pupil. However, some sub-apertures would be a lack of light induced by the imperfectness of micro-lenslets or pupil shift away from the optical path. Thus, the wavefront detection would be under-sampled and the performance of wavefront reconstruction would be severely degraded. It is therefore important to evaluate the influence of under-sampling on the wavefront reconstruction. In this paper, an AO system was established by the OOMAO simulation platform. For dynamical turbulence aberrations or statistic defocus aberrations, three cases including a single sub-aperture, a row of sub-apertures, and a quadrant sub-apertures lack of light were simulated. Compared with the uncorrected aberrant wavefront, our results showed that the RMS of the residual wavefront for a typical atmospheric condition (Fried parameter (r0) ranges from 5 cm to 15 cm) can be reduced by a factor of 5~8, 4~6, and 2~3 with these three cases of under-sampling, respectively.
Graphene-Assisted Electromagnetically Induced Transparency-like Terahertz Metabiosensor for Ultra-Sensitive Detection of Ovalbumin
Authors: Xu Ruochen , Bing Pibin , Yan Xin , Yao Haiyun , Liang Lanju , Li Zhenhua , Wang Ziqun , Hu Xiaofei , Wang Meng , Yao Jianquan
Published: January 2023

Terahertz (THz) metamaterial (MM) biosensors are a potential method of biomolecule detection. However, there have been few reports on the detection of trace proteins. In this study, we designed a novel THz biosensor consisting of graphene, polyimide (PI), and electromagnetically induced transparency-like (EIT-like) MMs for the ultra-sensitive detection of ovalbumin (OVA). The doping analyte can influence the Fermi level and electrical conductivity of graphene, as well as the coupling of resonators in MMs. These changes are reflected in the magnitude, phase, and frequency changes in the transmission spectra. The biosensor achieved a high sensitivity function for OVA and reached a limit of detection (LoD) of 8.63 pg/mL. The results showed that by regulating the Fermi level of graphene between the valence band, Dirac point, and conduction band, the sensitivity and LoD of MM-based THz biosensors can be enhanced. Such biosensors have the potential to be used in the high-sensitivity detection of trace proteins in biomedical fields.
Optimal Measurement of Telecom Wavelength Single Photon Polarisation via Hong-Ou-Mandel Interferometry
Authors: Sgobba Fabrizio , Pallotti Deborah Katia , Elefante Arianna , Dello Russo Stefano , Dequal Daniele , Siciliani de Cumis Mario , Santamaria Amato Luigi
Published: January 2023

The use of statistical estimation theory to boost the metrological performance of the measurement apparatus is becoming increasingly popular in a wide range of applications. Recently, such an approach has been adopted in Hong Ou Mandel interferometry, setting a new record in time delay and polarization measurement. Here, we extend these pioneering experiments in the telecom range to unlock the full potential of the information-based approach combined with a versatile spectral range, aiming for its adoption in fiber-coupled devices of up to hundreds of kilometers long as bobines or optical networks. Our measurement saturates the Cramér-Rao bound and in a long lasting experiment returns an Allan deviation of the polarization angle of 0.002 degs in 1 h of integration time.
Differences in Root Morphologies of Contrasting Wheat (Triticum aestivum) Genotypes Are Robust of a Drought Treatment
Authors: Si Zhuanyun , Delhaize Emmanuel , Hendriks Pieter-Willem , Li Xiaoqing
Published: January 2023

We aimed to assess the effect of water deprivation on root traits and to establish if the wheat cultivars Spica and Maringa would be useful as parental germplasm for a genetic analysis of root traits. Plants were grown in two markedly different soils under well-watered and water-limited treatments in controlled environment growth cabinets. The drought treatment was imposed as a gradual depletion of water over 28 days as seedlings grew from a defined starting moisture content. The root traits analyzed included length, nodal root number, thickness and nodal root angle. The relative differences in traits between genotypes generally proved to be robust in terms of water treatment and soil type. Maringa had a shallower nodal root angle than Spica, which was driven by the nodal roots. By contrast, the seminal roots of Maringa were found to be similar to or even steeper than those of Spica. We conclude that the differences in root traits between Spica and Maringa were robust to the drought treatment and soil types. Phenotyping on well-watered soil is relevant for identifying traits potentially involved in conferring water use efficiency. Furthermore, Spica and Maringa are suitable parental germplasm for developing populations to determine the genetics of key root traits.
The Impact of Solid-Phase Fermentation on Flavonoids, Phenolic Acids, Tannins and Antioxidant Activity in Chamerion angustifolium (L.) Holub (Fireweed) Leaves
Authors: Lasinskas Marius , Jariene Elvyra , Vaitkeviciene Nijole , Kulaitiene Jurgita , Adamaviciene Aida , Hallmann Ewelina
Published: January 2023

At present, the consumption of medical plants and functional foods is growing across the whole world. Fireweed (Chamerion angustifolium (L.) Holub), an important medicinal plant that has various pharmacological effects (antioxidant, anti-inflammatory, anticancer and others), can improve the state of health and well-being and reduce the risk of various diseases. The aim of this work was to investigate polyphenols (flavonoids, phenolic acids and tannins) and antioxidant activity in fireweed leaves fermented for 24, 48 and 72 h in solid-phase fermentation under aerobic and anaerobic conditions. High-performance liquid chromatography (HPLC) for polyphenols and the spectrophotometric method based on quenching of stable colored radical (ABTS•+) for antioxidant activity determinations were used. The results showed that the highest amounts of total polyphenols, total flavonoids and tannin oenothein B in the dried matter were found after 72 h and the highest total phenolic acids after 48 h of anaerobic solid-phase fermentation. The highest antioxidant activity was found after 72 h of solid-phase fermentation under aerobic conditions.
Genetic Redundancy in Rye Shows in a Variety of Ways
Authors: Vershinin Alexander V. , Elisafenko Evgeny A. , Evtushenko Elena V.
Published: January 2023

Fifty years ago Susumu Ohno formulated the famous C-value paradox, which states that there is no correlation between the physical sizes of the genome, i.e., the amount of DNA, and the complexity of the organism, and highlighted the problem of genome redundancy. DNA that does not have a positive effect on the fitness of organisms has been characterized as “junk or selfish DNA”. The controversial concept of junk DNA remains viable. Rye is a convenient subject for yet another test of the correctness and scientific significance of this concept. The genome of cultivated rye, Secale cereale L., is considered one of the largest among species of the tribe Triticeae and thus it tops the average angiosperm genome and the genomes of its closest evolutionary neighbors, such as species of barley, Hordeum (by approximately 30–35%), and diploid wheat species, Triticum (approximately 25%). The review provides an analysis of the structural organization of various regions of rye chromosomes with a description of the molecular mechanisms contributing to their size increase during evolution and the classes of DNA sequences involved in these processes. The history of the development of the concept of eukaryotic genome redundancy is traced and the current state of this problem is discussed.
Canopy Density, but Not Bacterial Titers, Predicts Fruit Yield in Huanglongbing-Affected Sweet Orange Trees
Authors: Levy Amit , Livingston Taylor , Wang Chunxia , Achor Diann , Vashisth Tripti
Published: January 2023

In Florida, almost all citrus trees are affected with Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas). We characterized various parameters of HLB-affected sweet orange trees in response to yield-improving nutritional treatment, including canopy volume, canopy density and CLas Ct values, and found that the treatment improved yield and maintained canopy density for over three years, whereas untreated HLB-affected trees declined in canopy density. The nutritional treatment did not affect CLas titer or the tree canopy volume suggesting that canopy density is a better indicator of fruit yield. To further validate the importance of canopy density, we evaluated three independent orchards (different in tree age or variety) to identify the specific traits that are correlated with fruit yields. We found that canopy density and fruit detachment force (FDF), were positively correlated with fruit yields in independent trials. Canopy density accurately distinguished between mild and severe trees in three field trials. High and low producing HLB trees had the same Ct values. Ct values did not always agree with CLas number in the phloem, as visualized by transmission electron microscopy. Our work identifies canopy density as an efficient trait to predict yields of HLB-affected trees and suggests canopy health is more relevant for yields than the CLas population.
Biological Nanofertilizers to Enhance Growth Potential of Strawberry Seedlings by Boosting Photosynthetic Pigments, Plant Enzymatic Antioxidants, and Nutritional Status
Authors: El-Bialy Said M. , El-Mahrouk Mohammed E. , Elesawy Taha , Omara Alaa El-Dein , Elbehiry Fathy , El-Ramady Hassan , Áron Béni , Prokisch József , Brevik Eric C. , Solberg Svein Ø.
Published: January 2023

Strawberry production presents special challenges due the plants’ shallow roots. The rooting stage of strawberry is a crucial period in the production of this important crop. Several amendments have been applied to support the growth and production of strawberry, particularly fertilizers, to overcome rooting problems. Therefore, the current investigation was carried out to evaluate the application of biological nanofertilizers in promoting strawberry rooting. The treatments included applying two different nanofertilizers produced biologically, nano-selenium (i.e., 25, 50, 75, and 100 mg L−1) and nano-copper (i.e., 50 and 100 mg L−1), plus a control (untreated seedlings). The rooting of strawberry seedlings was investigated by measuring the vegetative growth parameters (root weight, seedling weight, seedling length, and number of leaves), plant enzymatic antioxidants (catalase, peroxidase, and polyphenol oxidase activity), and chlorophyll content and its fluorescence and by evaluating the nutritional status (content of nutrients in the fruit and their uptake). The results showed that the applied nanofertilizers improved the growth, photosynthetic pigments, antioxidant content, and nutritional status of the seedlings compared to the control. A high significant increase in nutrient contents reached to more than 14-fold, 6-fold, 5-folf, and 4-fold for Cu, Mn, N, and Se contents, respectively, due to the applied nanofertilizers compared with the control. The result was related to the biological roles of both Se and CuO in activating the many plant enzymes. Comparing the Se with the CuO nanofertilizer, Cu had the strongest effect, which was shown in the higher values in all studied properties. This study showed that nanofertilizers are useful to stimulate strawberry seedling growth and most likely would also be beneficial for other horticultural crops. In general, the applied 100 ppm of biological nano-Se or nano-CuO might achieve the best growth of strawberry seedlings under growth conditions in greenhouses compared to the control. Along with the economic dimension, the ecological dimension of biological nanofertilizers still needs more investigation.
Genome-Wide Association Study and Genomic Prediction on Plant Architecture Traits in Sweet Corn and Waxy Corn
Authors: Dang Dongdong , Guan Yuan , Zheng Hongjian , Zhang Xuecai , Zhang Ao , Wang Hui , Ruan Yanye , Qin Li
Published: January 2023

Sweet corn and waxy corn has a better taste and higher accumulated nutritional value than regular maize, and is widely planted and popularly consumed throughout the world. Plant height (PH), ear height (EH), and tassel branch number (TBN) are key plant architecture traits, which play an important role in improving grain yield in maize. In this study, a genome-wide association study (GWAS) and genomic prediction analysis were conducted on plant architecture traits of PH, EH, and TBN in a fresh edible maize population consisting of 190 sweet corn inbred lines and 287 waxy corn inbred lines. Phenotypic data from two locations showed high heritability for all three traits, with significant differences observed between sweet corn and waxy corn for both PH and EH. The differences between the three subgroups of sweet corn were not obvious for all three traits. Population structure and PCA analysis results divided the whole population into three subgroups, i.e., sweet corn, waxy corn, and the subgroup mixed with sweet and waxy corn. Analysis of GWAS was conducted with 278,592 SNPs obtained from resequencing data; 184, 45, and 68 significantly associated SNPs were detected for PH, EH, and TBN, respectively. The phenotypic variance explained (PVE) values of these significant SNPs ranged from 3.50% to 7.0%. The results of this study lay the foundation for further understanding the genetic basis of plant architecture traits in sweet corn and waxy corn. Genomic selection (GS) is a new approach for improving quantitative traits in large plant breeding populations that uses whole-genome molecular markers. The marker number and marker quality are essential for the application of GS in maize breeding. GWAS can choose the most related markers with the traits, so it can be used to improve the predictive accuracy of GS.
New Insights into the Mechanism of Spatiotemporal Scent Accumulation in Orchid Flowers
Authors: Zheng Bao-Qiang , Li Xiao-Qing , Wang Yan
Published: January 2023

Orchid flowers have a unique structure that consists of three sepals and three petals, with one of the petals forming the labellum (lip) that can be differentiated into the hypochile and epichile. In orchids, the emission of floral scent is specific and spatially complex. Little is understood about the molecular and biochemical mechanisms of the differing scent emissions between the parts of orchid flowers. Here, we investigated this in the Cattleya hybrid KOVA, and our study showed that monoterpenes, including linalool and geraniol, are the main components responsible for the KOVA floral scent. The KOVA flower was scentless to the human nose before it reached full bloom, potentially because the 1-deoxy-d-xylulose 5-phosphate synthases (RcDXSs) and 4-hydroxy-3-methylbut-2-enyl diphosphate synthases (RcHDSs) that biosynthesize monoterpenes were highly expressed in flowers only when it reached full flowering. Additionally, the spatial expression profile of the monoterpene synthases (RcMTPSs), which were highly expressed in the basal region of the lip (hypochile), contributed to the highest monoterpene emissions from this part of the flower. This might have caused the hypochile to be more fragrant than the other parts of the flower. These findings enrich our understanding of the difference in scents between different flower parts in plants and provide information to breed novel orchid cultivars with special floral scents.
Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review
Authors: Nascimento Fernanda dos Santos , Rocha Anelita de Jesus , Soares Julianna Matos da Silva , Mascarenhas Marcelly Santana , Ferreira Mileide dos Santos , Morais Lino Lucymeire Souza , Ramos Andresa Priscila de Souza , Diniz Leandro Eugenio Cardamone , Mendes Tiago Antônio de Oliveira , Ferreira Claudia Fortes , Santos-Serejo Janay Almeida dos , Amorim Edson Perito
Published: January 2023

Agricultural crops are exposed to various abiotic stresses, such as salinity, water deficits, temperature extremes, floods, radiation, and metal toxicity. To overcome these challenges, breeding programs seek to improve methods and techniques. Gene editing by Clustered Regularly Interspaced Short Palindromic Repeats—CRISPR/Cas—is a versatile tool for editing in all layers of the central dogma with focus on the development of cultivars of plants resistant or tolerant to multiple biotic or abiotic stresses. This systematic review (SR) brings new contributions to the study of the use of CRISPR/Cas in gene editing for tolerance to abiotic stress in plants. Articles deposited in different electronic databases, using a search string and predefined inclusion and exclusion criteria, were evaluated. This SR demonstrates that the CRISPR/Cas system has been applied to several plant species to promote tolerance to the main abiotic stresses. Among the most studied crops are rice and Arabidopsis thaliana, an important staple food for the population, and a model plant in genetics/biotechnology, respectively, and more recently tomato, whose number of studies has increased since 2021. Most studies were conducted in Asia, specifically in China. The Cas9 enzyme is used in most articles, and only Cas12a is used as an additional gene editing tool in plants. Ribonucleoproteins (RNPs) have emerged as a DNA-free strategy for genome editing without exogenous DNA. This SR also identifies several genes edited by CRISPR/Cas, and it also shows that plant responses to stress factors are mediated by many complex-signaling pathways. In addition, the quality of the articles included in this SR was validated by a risk of bias analysis. The information gathered in this SR helps to understand the current state of CRISPR/Cas in the editing of genes and noncoding sequences, which plays a key role in the regulation of various biological processes and the tolerance to multiple abiotic stresses, with potential for use in plant genetic improvement programs.
Wild Stinging Nettle (Urtica dioica L.) Leaves and Roots Chemical Composition and Phenols Extraction
Authors: Tarasevičienė Živilė , Vitkauskaitė Miglė , Paulauskienė Aurelija , Černiauskienė Judita
Published: January 2023

Stinging nettle (Urtica dioica L.) is an herbaceous plant that grows all over the world and is widely used as an edible and medicinal plant. Overall research results reveal that the chemical content and antioxidant activity of aerial parts and roots of stinging nettle depends on the growing region, soil, meteorological conditions (especially sunshine), collecting time, etc. The chemical composition of stinging nettle growing in Lithuania and the solid–liquid extraction efficiency of leaves and roots using different solvents were analysed. Additionally, we determined leaves phenols extraction efficiency using 96% methanol at different extraction conditions. Research results showed that a higher amount of crude fats, non-nitrogen extractives, and total carotenoids were in leaves, but the amount of crude proteins and ash did not differ significantly compared with roots. A higher amount of polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs) were detected in roots instead of leaves while saturated fatty acids (SFAs) were in leaves. The extraction results showed that the most effective solvent for total phenols and flavonoids in leaves was 96% methanol, for total phenols in roots was 50% methanol and 50% ethanol for total flavonoids in roots. The most effective temperature for the Urtica dioica L. leaves phenols extraction was 70 °C, while time does not have a significant influence. The present study’s findings suggested that concentrated and binary solvents had different effects on the phenol’s extraction efficiency from different stinging nettle parts and extraction temperature performed a key role instead of extraction time.
Influence of Nitrogen Application Rate on the Importance of NO3−-N and NH4+-N Transfer via Extramycelia of Arbuscular Mycorrhiza to Tomato with Expression of LeNRT2.3 and LeAMT1.1
Authors: Xie Xiaocan , Huang Zhe , Lv Weixing , Zhu Houteng , Hui Guoming , Li Ronghua , Lei Xihong , Li Zhifang
Published: January 2023

Arbuscular mycorrhizal fungi (AMF) form mutualistic symbiotic relationships with many land plants and play a key role in nitrogen (N) acquisition. NO3−-N and NH4+-N are the main sources of soil mineral N, but how extraradical mycelial transfer affects the different N forms and levels available to tomato plants is not clear. In the present study, we set up hyphal compartments (HCs) to study the efficiency of N transfer from the extramycelium to tomato plants treated with different N forms and levels of fertilization. Labeled 15NO3−-N or 15NH4+-N was placed in hyphal compartments under high and low N application levels. 15N accumulation in shoots and the expression of LeNRT2.3, LeAMT1.1, and LeAMT1.2 in the roots of tomato were measured. According to our results, both 15NO3−-N and 15NH4+-N were transported via extraradical mycelia to the shoots of plants. 15N accumulation in shoots was similar, regardless of the N form, while a higher 15N concentration was found in shoots with low N application. Compared with the control, inoculation with AMF significantly increased the expression of LeAMT1.1 under high N and LeNRT2.3 under low N. The expression of LeAMT1.1 under high N was significantly increased when NO3—N was added, while the expression of LeNRT2.3 was significantly increased when NH4+-N was added under low N. Taken together, our results suggest that the N transfer by extraradical mycelia is crucial for the acquisition of both NO3−-N and NH4+-N by the tomato plant; however, partial N accumulation in plant tissue is more important with N deficiency compared with a higher N supply. The expression of N transporters was influenced by both the form and level of N supply.
An Appropriate Approach to Recognize Coke Size Distribution in a Blast Furnace
Authors: Wang Xu , Guo Yanling , Yu Yaowei
Published: January 2023

The size distribution of coke is important in order to decide the burden layer structure and the burden porosity in the shaft of a blast furnace (BF), which fluctuates daily and can be determined by several parameters. It is measured two or three times per shift by screening the raw material. However, the screening method used is random and takes a lot of time and manpower, resulting in the susceptive size distribution of the raw material and delayed operation of the BF. Therefore, in this paper, a new online approach used to measure the size distribution of particles was selected through comparison. Four common algorithms were used to detect the coke particles from images, including the Marker-based Watershed (MW), Histogram of Oriented Gradient + Support Vector Machine (HOG + SVM), Faster Region-based Convolutional Neural Networks (Faster R-CNN), and You Only Look Once (YOLOv3). The results show that the MW and HOG + SVM were not suitable for coke image detection. The average mean average precisions (mAPs) of Faster R-CNN and YOLOv3 were 93.391% and 91.348%, respectively, which meet the requirements of coke particle recognition. However, the YOLOv3 (5.419 fps) was selected as the final coke particle image detection algorithm, which is about 4.3 times faster than the average detection speed of Faster RCNN (1.269 fps). After this, the YOLOv3 and screening were used to detect 100 coke images and to generate particle size distribution statistics. The results show that the two methods are basically consistent. YOLOv3 can be used in the online measurement of BF coke. This research, which is of important value, provides a basis for the online measurement of the particle size distribution of raw material in a BF.
Copper Recovery from Aqueous Solutions by Hemp Shives: Adsorption Studies and Modeling
Authors: Mongioví Chiara , Crini Grégorio
Published: January 2023

This article describes the performance of hemp shives, a co-product of the hemp industry, when used as an adsorbent to recover copper present in aqueous solutions by a batch method. Two materials provided by an industrial partner, namely water-washed hemp shives (SHI-W) and Na2CO3-activated shives (SHI-C), were studied. Two important variables in the water treatment industry, i.e., pH and ionic strength of the solution, were studied to evaluate their impact on the purification performance of the materials. The results obtained clearly indicated that the performance in terms of Cu removal obtained from the SHI-C material was significantly higher than that obtained with SHI-W, mainly due to the structural and chemical modifications after carbonate treatment. For each dose of this adsorbent, a percentage of recovery between 90 and 100% was achieved, independently of the pH value in the range 3–5. In the case of SHI-W, the highest values were between 60 and 75% and were both dependent on the pH and the dose used. SHI-C was also able to selectively remove 70% of copper in the presence of NaCl 1 M. The analysis of the isotherms indicated the presence of a complex adsorption mechanism that cannot be described by only one isotherm model.