bluuebunny/crossref_metadata_2025 huggingface dataset:
Distributed Model Predictive Longitudinal Control for a Connected Autonomous Vehicle Platoon with Dynamic Information Flow Topology
Authors: Zhao Fei , Liu Yu , Wang Jian , Wang Li
Published: August 2021

The development of vehicle-to-vehicle (V2V) improves the cooperation efficiency of the connected autonomous vehicle (CAV) platoon. However, the failure of the network communication occurs occasionally in the realistic environment, where the ideal fixed information flow topology (FIFT) cannot be adapted. To address this issue, this paper proposes a dynamic information flow topology (DIFT) utilizing a distributed model predictive control (DMPC) algorithm for CAV platoons. When the communication link is broken, the platoon control system will switch to the corresponding collaborative control mode instead of the degeneration to adaptive cruise control (ACC). First, the duty-vehicle dynamic model is constructed. In addition, the constraints with vehicle physical limitations and rear-end collision are considered. The acceleration information of the pedal actuator from the leading vehicle and the states of the predecessor including position, velocity and acceleration are transmitted to the following vehicle with a switch Indicator under DIFT. The cost function with the consideration of DIFT and fuel consumption is formulated for the optimization problem. Comparing with the FIFT, the proposed method is evaluated in the co-simulation of Matlab-TruckSim. The results demonstrate that the proposed DIFT strategy shows the satisfactory performance of the platoon under the communication issues by measuring inter-vehicle space, position and velocity tracking, and acceleration change with high tracking accuracy of position within 1.2 m and velocity within 0.04 m/s.
Impacts of Divergent Moving Drives on Energy Efficiency and Performance of Various AMS in Operative Conditions
Authors: Höhendinger Martin , Krieg Hans-Jürgen , Dietrich Reinhard , Rauscher Stefan , Stumpenhausen Jörn , Bernhardt Heinz
Published: August 2021

In recent decades, the costs of energy in dairy farming increased mainly due to rising energy prices but also due to increased mechanisation and automatisation. Electric energy in dairy farming is essentially used for milking and milk cooling. However, the energy consumption of automatic milking systems (AMS) depend on many factors (e.g., machine generations, machine configurations and settings, and operative conditions). To evaluate the differences in performance and energy efficiency of AMS, the impact of different technologies within the attachment arm in practical conditions, a detailed quantification of energy consumption was carried out on two consecutive single box automatic milking systems (AMS) of a dairy farm in southern Bavaria (Germany). The AMS equipped with an electrical drive of the attachment arm was more efficient and showed a higher capacity regarding cows in the herd. The replacement of the pneumatic drive with electrical drives leads to higher energy consumptions of the milking robot but reduces the energy consumption of the air compressor. Hence, the energy efficiency of the electric attachment arm showed strong advantages in the energetic efficiency of the whole milking process. Advances of sustainability due to the increased performance are and should be investigated in further research.
Effects of Dietary Supplementation of Salvia officinalis L. in Organic Laying Hens on Egg Quality, Yolk Oxidative Stability and Eggshell Microbiological Counts
Authors: Galamatis Dimitrios , Papadopoulos Georgios A. , Lazari Diamanto , Fletouris Dimitrios , Petridou Evanthia , Arsenos Georgios I. , Fortomaris Paschalis
Published: August 2021

Aromatic plants of Labiatae family are used in poultry diets because of their antimicrobial and antioxidant activity. The notion is that hens raised in organic systems face several health and environmental challenges. Hence, the objective here was to assess hens’ performances and the quality of their eggs in such systems following a dietary supplementation of Salvia officinalis L. in powder form. The experiments were conducted over two successive years (1 and 2). They lasted 16 weeks each and involved 198 laying hens aged 40 weeks old randomly assigned to three groups: Con (control diet), Sal-0.5%, and Sal-1.0% (diets supplemented with Salvia officinalis L. at 0.5% or 1.0%, respectively). The malondialdehyde (MDA) levels in egg yolks in year 2 were lower in both Sal-0.5% and Sal-1.0% compared to the Con (p < 0.05). The total number of Enterobacteriaceae in eggshells were lower in Sal-1.0% compared to the Con (p < 0.05) in both years. The results suggest that a dietary supplementation of Salvia officinalis L. at 1.0% improves the antioxidant status and reduces the microbial load of eggs produced in organic systems.
Reproductive Management of Rams and Ram Lambs during the Pre-Breeding Season in US Sheep Farms
Authors: Maquivar Martin G. , Smith Sarah M. , Busboom Jan R.
Published: August 2021

In sheep farms, achieving economic and productive efficiency and sustainability goals is directly related with the reproductive management of the animals. Genetically, the male contribution to the offspring is 50%, but in practical terms, there is a greater potential impact of the ram on reproductive results, because one male has the potential to affect a large group of females and therefore greatly affects the entire flock. Unfortunately, the selection of males for breeding in the US sheep industry has been traditionally conducted based on phenotypical traits, without a genetic/reproductive evaluation, and/or health assessment or records. Therefore, it is important to establish integrative management practices to select the rams and ram lambs to be used in the breeding season. Among these practices are genomic testing, a comprehensive breeding soundness evaluation and assessment of health status and records of the males before the breeding season, to detect and correct potential issues.
Comprehensive Proteomic Analysis Revealed a Large Number of Newly Identified Proteins in the Small Extracellular Vesicles of Milk from Late-Stage Lactating Cows
Authors: Rahman Md. Matiur , Takashima Shigeo , Kamatari Yuji O. , Shimizu Kaori , Okada Ayaka , Inoshima Yasuo
Published: August 2021

Bovine milk contains small extracellular vesicles (sEVs) that provide proteins, miRNAs, mRNAs, DNAs, and lipids to target cells and play a role in intracellular communications. Previous studies have characterized proteins in milk sEVs from early- and mid-stage lactation. However, the proteins in milk sEVs from late-stage lactation are mostly unexplored. The aim of this study was to determine the proteomic profile of milk sEVs from late-stage lactating cows. A comprehensive nanoliquid chromatography–tandem mass spectrometry (nanoLC-MS/MS) approach was carried out to reveal the proteins in milk sEVs. Additionally, bioinformatics analysis was carried out to interpret the molecular signatures of newly identified proteins in milk sEVs from three late-stage lactating cows. NanoLC-MS/MS analysis revealed a total of 2225 proteins in milk sEVs from cows. Notably, after comparing these identified proteins with previously deposited datasets of proteins in bovine milk sEVs, 429 proteins were detected as newly identified. Bioinformatic analysis indicated that these newly identified proteins in milk sEVs were engaged in a diverse range of molecular phenomena relevant to mammary gland physiology, milk production, immunity, and immune response. These findings suggest that the newly identified proteins could expand the inventory application of molecular cargos, nutritional status, and immune modulation of sEVs in milk during the late-stage lactation.
Differential Effects of Polyphenols on Insulin Proteolysis by the Insulin-Degrading Enzyme
Authors: Zheng Qiuchen , Kebede Micheal T. , Lee Bethany , Krasinski Claire A. , Islam Saadman , Wurfl Liliana A. , Kemeh Merc M. , Ivancic Valerie A. , Jakobsche Charles E. , Spratt Donald E. , Lazo Noel D.
Published: August 2021

The insulin-degrading enzyme (IDE) possesses a strong ability to degrade insulin and Aβ42 that has been linked to the neurodegeneration in Alzheimer’s disease (AD). Given this, an attractive IDE-centric strategy for the development of therapeutics for AD is to boost IDE’s activity for the clearance of Aβ42 without offsetting insulin proteostasis. Recently, we showed that resveratrol enhances IDE’s activity toward Aβ42. In this work, we used a combination of chromatographic and spectroscopic techniques to investigate the effects of resveratrol on IDE’s activity toward insulin. For comparison, we also studied epigallocatechin-3-gallate (EGCG). Our results show that the two polyphenols affect the IDE-dependent degradation of insulin in different ways: EGCG inhibits IDE while resveratrol has no effect. These findings suggest that polyphenols provide a path for developing therapeutic strategies that can selectively target IDE substrate specificity.
NAC and Vitamin D Improve CNS and Plasma Oxidative Stress in Neonatal HIE and Are Associated with Favorable Long-Term Outcomes
Authors: Jenkins Dorothea D , Moss Hunter G , Brown Truman R , Yazdani Milad , Thayyil Sudhin , Montaldo Paolo , Vento Maximo , Kuligowski Julia , Wagner Carol , Hollis Bruce W , Wiest Donald B
Published: August 2021

N-acetylcysteine (NAC) and vitamin D provide effective neuroprotection in animal models of severe or inflammation-sensitized hypoxic ischemic encephalopathy (HIE). To translate these FDA-approved drugs to HIE neonates, we conducted an early phase, open-label trial of 10 days of NAC (25, 40 mg/kg q12h) + 1,25(OH)2D (calcitriol 0.05 mg/kg q12h, 0.03 mg/kg q24h), (NVD), for pharmacokinetic (PK) estimates during therapeutic hypothermia and normothermia. We paired PK samples with pharmacodynamic (PD) targets of plasma isoprostanoids, CNS glutathione (GSH) and total creatine (tCr) by serial MRS in basal ganglia (BG) before and after NVD infusion at five days. Infants had moderate (n = 14) or severe HIE (n = 16), funisitis (32%), and vitamin D deficiency (75%). NVD resulted in rapid, dose-responsive increases in CNS GSH and tCr that correlated positively with plasma [NAC], inversely with plasma isofurans, and was greater in infants with lower baseline [GSH] and [tCr], suggesting increases in these PD markers were titrated by neural demand. Hypothermia and normothermia altered NAC PK estimates. NVD was well tolerated. Excluding genetic syndromes (2), prolonged ECMO (2), lost-to-follow-up (1) and SIDS death (1), 24 NVD treated HIE infants have no evidence of cerebral palsy, autism or cognitive delay at 24–48 months. These data confirm that low, safe doses of NVD in HIE neonates decreased oxidative stress in plasma and CNS, improved CNS energetics, and are associated with favorable developmental outcomes at two to four years.
Necrostatin-1 Prevents Ferroptosis in a RIPK1- and IDO-Independent Manner in Hepatocellular Carcinoma
Authors: Yuk Hanna , Abdullah Md , Kim Do-Hyung , Lee Haeseung , Lee Seung-Jin
Published: August 2021

Ferroptosis is caused by the iron-mediated accumulation of lipid peroxidation, which is distinct from apoptosis and necroptosis. Necrostatin-1 inhibits receptor-interacting serine/threonine-protein kinase 1 (RIPK1) to initiate necroptosis; it also inhibits indoleamine 2,3-dioxygenase (IDO) to regulate tumor immunity. However, few studies have examined the off-target effect of necrostatin-1 on the ferroptosis pathway. The present study examined whether necrostatin-1 could interrupt ferroptosis induced by system xc- inhibitors (sulfasalazine and erastin) and a glutathione peroxidase 4 inhibitor (RSL3) in Huh7 and SK-HEP-1 cells. Necrostatin-1 completely prevented decreases in cell viability induced by sulfasalazine and erastin; it partially blunted decreases in cell viability induced by RSL3. Necrostatin-1, ferrostatin-1, and deferoxamine repressed sulfasalazine-provoked membrane permeabilization, as detected by 7-aminoactinomycin D staining and lipid peroxidation measured using a C11-BODIPY probe. However, other RIPK1 inhibitors (necrostatin-1s and GSK2982772) and an IDO inhibitor (1-methyl-D-tryptophan) did not recover the decrease in cell viability induced by sulfasalazine. Necrostatin-1 potentiated sulfasalazine-induced expression of xCT, a catalytic subunit of system xc- in these cells. These results demonstrated that necrostatin-1 blocked ferroptosis through a mechanism independent from RIPK1 and IDO inhibition in Huh7 and SK-HEP-1 cells, indicating that its antioxidant activity should be considered when using necrostatin-1 as a RIPK1 inhibitor.
Comparative Cytotoxic Activity of Hydroxytyrosol and Its Semisynthetic Lipophilic Derivatives in Prostate Cancer Cells
Authors: León-González Antonio J. , Sáez-Martínez Prudencio , Jiménez-Vacas Juan M. , Herrero-Aguayo Vicente , Montero-Hidalgo Antonio J. , Gómez-Gómez Enrique , Madrona Andrés , Castaño Justo P. , Espartero José L. , Gahete Manuel D. , Luque Raúl M.
Published: August 2021

A high adherence to a Mediterranean diet has been related to numerous beneficial effects in human health, including a lower incidence and mortality of prostate cancer (PCa). Olive oil is an important source of phenolic bioactive compounds, mainly hydroxytyrosol (HT), of this diet. Because of the growing interest of this compound and its derivatives as a cancer chemopreventive agent, we aimed to compare the in vitro effect of HT isolated from olive mill wastewaters and five semisynthetic alkyl ether, ester, and nitro-derivatives against prostate cancer (PCa) cell lines. The effect in cell proliferation was determined in RWPE-1, LNCaP, 22Rv1, and PC-3 cells by resazurin assay, the effect in cell migration by wound healing assay, and tumorsphere and colony formation were evaluated. The changes in key signaling pathways involved in carcinogenesis were assessed by using a phosphorylation pathway profiling array and by Western blotting. Antiproliferative effects of HT and two lipophilic derivatives [hydroxytyrosyl acetate (HT-Ac)/ethyl hydroxytyrosyl ether (HT-Et)] were significantly higher in cancerous PC-3 and 22Rv1 cells than in non-malignant RWPE-1 cells. HT/HT-Ac/HT-Et significantly reduced migration capacity in RWPE-1 and PC-3 and prostatosphere size and colony formation in 22Rv1, whereas only HT-Ac and HT-Et reduced these functional parameters in PC-3. The cytotoxic effect in 22Rv1 cells was correlated with modifications in the phosphorylation pattern of key proteins, including ERK1/2 and AKT. Consistently, HT-Ac and HT-Et decreased p-AKT levels in PC-3. In sum, our results suggest that HT and its lipophilic derivatives could be considered as potential therapeutic tools in PCa.
Role of Oxidative Damage in Alzheimer’s Disease and Neurodegeneration: From Pathogenic Mechanisms to Biomarker Discovery
Authors: Buccellato Francesca Romana , D’Anca Marianna , Fenoglio Chiara , Scarpini Elio , Galimberti Daniela
Published: August 2021

Alzheimer’s disease (AD) is a neurodegenerative disorder accounting for over 50% of all dementia patients and representing a leading cause of death worldwide for the global ageing population. The lack of effective treatments for overt AD urges the discovery of biomarkers for early diagnosis, i.e., in subjects with mild cognitive impairment (MCI) or prodromal AD. The brain is exposed to oxidative stress as levels of reactive oxygen species (ROS) are increased, whereas cellular antioxidant defenses are decreased. Increased ROS levels can damage cellular structures or molecules, leading to protein, lipid, DNA, or RNA oxidation. Oxidative damage is involved in the molecular mechanisms which link the accumulation of amyloid-β and neurofibrillary tangles, containing hyperphosphorylated tau, to microglia response. In this scenario, microglia are thought to play a crucial role not only in the early events of AD pathogenesis but also in the progression of the disease. This review will focus on oxidative damage products as possible peripheral biomarkers in AD and in the preclinical phases of the disease. Particular attention will be paid to biological fluids such as blood, CSF, urine, and saliva, and potential future use of molecules contained in such body fluids for early differential diagnosis and monitoring the disease course. We will also review the role of oxidative damage and microglia in the pathogenesis of AD and, more broadly, in neurodegeneration.
Bioprinting of a Zonal-Specific Cell Density Scaffold: A Biomimetic Approach for Cartilage Tissue Engineering
Authors: Dimaraki Angeliki , Díaz-Payno Pedro J. , Minneboo Michelle , Nouri-Goushki Mahdiyeh , Hosseini Maryam , Kops Nicole , Narcisi Roberto , Mirzaali Mohammad J. , van Osch Gerjo J. V. M. , Fratila-Apachitei Lidy E. , Zadpoor Amir A.
Published: August 2021

The treatment of articular cartilage defects remains a significant clinical challenge. This is partially due to current tissue engineering strategies failing to recapitulate native organization. Articular cartilage is a graded tissue with three layers exhibiting different cell densities: the superficial zone having the highest density and the deep zone having the lowest density. However, the introduction of cell gradients for cartilage tissue engineering, which could promote a more biomimetic environment, has not been widely explored. Here, we aimed to bioprint a scaffold with different zonal cell densities to mimic the organization of articular cartilage. The scaffold was bioprinted using an alginate-based bioink containing human articular chondrocytes. The scaffold design included three cell densities, one per zone: 20 × 106 (superficial), 10 × 106 (middle), and 5 × 106 (deep) cells/mL. The scaffold was cultured in a chondrogenic medium for 25 days and analyzed by live/dead assay and histology. The live/dead analysis showed the ability to generate a zonal cell density with high viability. Histological analysis revealed a smooth transition between the zones in terms of cell distribution and a higher sulphated glycosaminoglycan deposition in the highest cell density zone. These findings pave the way toward bioprinting complex zonal cartilage scaffolds as single units, thereby advancing the translation of cartilage tissue engineering into clinical practice.
Review of Image-Based 3D Reconstruction of Building for Automated Construction Progress Monitoring
Authors: Xue Jingguo , Hou Xueliang , Zeng Ying
Published: August 2021

With the spread of camera-equipped devices, massive images and videos are recorded on construction sites daily, and the ever-increasing volume of digital images has inspired scholars to visually capture the actual status of construction sites from them. Three-dimensional (3D) reconstruction is the key to connecting the Building Information Model and the project schedule to daily construction images, which enables managers to compare as-planned with as-built status and detect deviations and therefore monitor project progress. Many scholars have carried out extensive research and produced a variety of intricate methods. However, few studies comprehensively summarize the existing technologies and introduce the homogeneity and differences of these technologies. Researchers cannot clearly identify the relationship between various methods to solve the difficulties. Therefore, this paper focuses on the general technical path of various methods and sorts out a comprehensive research map, to provide reference for researchers in the selection of research methods and paths. This is followed by identifying gaps in knowledge and highlighting future research directions. Finally, key findings are summarized.
Comparative Study and Overview of Field-Oriented Control Techniques for Six-Phase PMSMs
Authors: Furmanik Marek , Gorel Lukáš , Konvičný Daniel , Rafajdus Pavol
Published: August 2021

This paper targets the main current trend in automotive motor control applications, i.e., six-phase permanent−magnet synchronous machines. Multiphase machines have been studied for more than one decade, but they are currently becoming more and more important for automotive technology. Increased safety and improved reliability are the two main reasons why six-phase machines conquer safety-critical motor control applications. The paper provides a detailed description, analysis, and comparison of two field-oriented control strategies for six-phase machines. The article consists of four main parts: (1) a general introduction of the application field of six-phase machines; (2) a description of two different field-oriented control techniques; (3) a presentation of the experimental results, e.g., frequency and step response analysis, as well as a comparison between a mathematical model and a real system; (4) a detailed comparison of strategies including pros and cons, with a strong focus on the main advantages.
Microbially Induced Desaturation and Carbonate Precipitation through Denitrification: A Review
Authors: Lin Wenbin , Lin Wei , Cheng Xiaohui , Chen Guozhou , Ersan Yusuf Cagatay
Published: August 2021

Microbially induced carbonate precipitation (MICP) has been proposed as a sustainable approach to solve various environmental, structural, geotechnical and architectural issues. In the last decade, a ubiquitous microbial metabolism, nitrate reduction (also known as denitrification) got attention in MICP research due to its unique added benefits such as simultaneous corrosion inhibition in concrete and desaturation of porous media. The latter even upgraded MICP into a more advanced concept called microbially induced desaturation and precipitation (MIDP) which is being investigated for liquefaction mitigation. In this paper, we present the findings on MICP through denitrification by covering applications under two main titles: (i) applications solely based on MICP, such as soil reinforcement, development of microbial self-healing concrete, restoration of artwork and historical monuments, and industrial wastewater treatment, (ii) an application based on MIDP: liquefaction mitigation. After explaining the denitrification process in detail and describing the MICP and MIDP reaction system occurring through denitrification metabolism, the most recent advances in each potential field of application are collected, addressing the novel findings and limitations, to provide insights toward the practical applications in situ. Finally, the research needs required to deal with the defined challenges in application-oriented upscaling and optimization of MICP through denitrification are suggested. Overall, collected research findings revealed that MICP through denitrification possesses a great potential to replace conventionally used petrochemical-based, labour intensive, destructive and economically unfeasible techniques used in construction industry with a bio-based, labourless, low-carbon technology. This worldwide applicable bio-based technology will facilitate the sustainable development and contribute to the carbon-emission-reduction.
Effect of Geometrical Factors on Torsion in Cold Roll Forming of the Lower Side Beam of a Car
Authors: Wang Yesong , Xu Xiaodong , Ren Bobo , Liu Jiang , Zhao Rongguo
Published: August 2021

Cold roll forming is a continuous metal forming process used to produce a large variety of profiles. A significant product of cold roll forming is asymmetric profiles, whose application is expanding in the construction of cars, houses, bridges, etc. Torsion is one of the common defects in the cold roll forming process of asymmetric section steel and sometimes seriously affects cold roll forming products. With the development of the economy and technology, many asymmetric cold roll-formed parts are being gradually applied to automobiles. Three-dimensional finite element analysis models of 12 stands with 700L sheet material were carried out using the professional COPRA RF and MSC MARC software, taking the lower beam of an automobile as the research object. Five geometric factors of the lower side beam were researched, and the influence rules of different geometric elements on the forming torsion were analyzed. We found that with the increase of web width, flange width, and vertical edge height and the decrease of the corner radius and strip thickness, the torsion defects of cold roll forming were improved to different degrees. The results also showed that the width of the flange has the most significant effect on torsion.
Characterization of the Driving Style by State–Action Semantic Plane Based on the Bayesian Nonparametric Approach
Authors: Qiao Xuqiang , Zheng Ling , Li Yinong , Ren Yuqing , Zhang Zhida , Zhang Ziwei , Qiu Lihong
Published: August 2021

The quantification and estimation of the driving style are crucial to improve the safety on the road and the acceptance of drivers with level2–level3(L2–L3) intelligent vehicles. Previous studies have focused on identifying the difference in driving style between categories, without further consideration of the driving behavior frequency, duration proportion properties, and the transition properties between driving style and behaviors. In this paper, a novel methodology to characterize the driving style is proposed by using the State–Action semantic plane based on the Bayesian nonparametric approach, i.e., hierarchical Dirichlet process–hidden semi–Markov model (HDP–HSMM). This method segments the time series driving data into fragment clusters with similar characteristics and construct the State–Action semantic plane based on the statistical characteristics of the state and action layer to label and interpret the fragment clusters. This intuitively and simply visualizes the driving performance of individual drivers, while the risk index of the individual drivers can also be obtained through semantic plane. In addition, according to the joint mutual information maximization (JIMI) approach, seven transition probabilities of driving behaviors are extracted from the semantic plane and applied to identify driving styles of drivers. We found that the aggressive drivers prefer high–risk driving behaviors, and the total duration and frequency of high–risk behaviors are greater than those of cautious and normal drivers. The transition probabilities among high–risk driving behaviors are also greater compared with low–risk behaviors. Moreover, the transition probabilities can provide rich information about driving styles and can improve the classification accuracy of driving styles effectively. Our study has practical significance for the regulation of driving behavior and improvement of road safety and the development of advanced driver assistance systems (ADAS).
Prognostic Value of the Immunohistochemical Expression of Serine and Arginine-Rich Splicing Factor 1 (SRSF1) in Uveal Melanoma: A Clinico-Pathological and Immunohistochemical Study on a Series of 85 Cases
Authors: Broggi Giuseppe , Falzone Luca , Fallico Matteo , Russo Andrea , Reibaldi Michele , Longo Antonio , Avitabile Teresio , De Pasquale Rocco , Puzzo Lidia , Foti Pietro Valerio , Russo Daniela , Di Crescenzo Rosa Maria , Libra Massimo , Staibano Stefania , Caltabiano Rosario
Published: August 2021

Uveal melanoma (UM) is the most frequent primary ocular malignancy of adults; it exhibits an almost invariably poor prognosis with onset of liver metastases within 10–15 years after the diagnosis. Serine and arginine-rich splicing factor 1 (SRSF1) is an RNA-binding protein with proto-oncogene functions, including stimulation of angiogenesis, cell migration and cell growth; regarding the complex regulation of tumor angiogenesis, it has been suggested that SRSF1 regulates the alternative splicing of vascular endothelial growth factor-α, promoting the formation of its pro-angiogenic isoform. The immunohistochemical expression of SRSF1 on a series of 85 primary UMs, including 39 metastasizing and 46 non-metastasizing cases, was investigated; to clarify the potential pathogenetic role of SRSF1 in this tumor and its effect on angiogenesis, we correlated our immunohistochemical findings with the clinico-pathological features, the prognostic data and blood vascular microvessel density (MVD) findings of the cases from our series. Cases with higher immunohistochemical expression of SRSF1 also had higher MVD, higher metastatic potential and shorter metastasis-free survival; conversely, cases with lower SRSF1 immunoexpression showed lower MVD, lower metastatic risk and longer metastasis-free survival times. Our results suggested that SRSF1 has a negative prognostic role and a pro-angiogenic function in UM.
Performance Evaluation of Ground AR Anchor with WebXR Device API
Authors: Lee Daehyeon , Shim Woosung , Lee Munyong , Lee Seunghyun , Jung Kye-Dong , Kwon Soonchul
Published: August 2021

Recently, the development of 3D graphics technology has led to various technologies being combined with reality, where a new reality is defined or studied; they are typically named by combining the name of the technology with “reality”. Representative “reality” includes Augmented Reality, Virtual Reality, Mixed Reality, and eXtended Reality (XR). In particular, research on XR in the web environment is actively being conducted. The Web eXtended Reality Device Application Programming Interface (WebXR Device API), released in 2018, allows instant deployment of XR services to any XR platform requiring only an active web browser. However, the currently released tentative version has poor stability. Therefore, in this study, the performance evaluation of WebXR Device API is performed using three experiments. A camera trajectory experiment is analyzed using ground truth, we checked the standard deviation between the ground truth and WebXR for the X, Y, and Z axes. The difference image experiment is conducted for the front, left, and right directions, which resulted in a visible difference image for each image of ground truth and WebXR, small mean absolute error, and high match rate. In the experiment for measuring the 3D rendering speed, a frame rate similar to that of real-time is obtained.
Insights into Alexandrium minutum Nutrient Acquisition, Metabolism and Saxitoxin Biosynthesis through Comprehensive Transcriptome Survey
Authors: Akbar Muhamad Afiq , Yusof Nurul Yuziana Mohd , Sahrani Fathul Karim , Usup Gires , Ahmad Asmat , Baharum Syarul Nataqain , Muhammad Nor Azlan Nor , Bunawan Hamidun
Published: August 2021

The toxin-producing dinoflagellate Alexandrium minutum is responsible for the outbreaks of harmful algae bloom (HABs). It is a widely distributed species and is responsible for producing paralytic shellfish poisoning toxins. However, the information associated with the environmental adaptation pathway and toxin biosynthesis in this species is still lacking. Therefore, this study focuses on the functional characterization of A. minutum unigenes obtained from transcriptome sequencing using the Illumina Hiseq 4000 sequencing platform. A total of 58,802 (47.05%) unigenes were successfully annotated using public databases such as NCBI-Nr, UniprotKB, EggNOG, KEGG, InterPRO and Gene Ontology (GO). This study has successfully identified key features that enable A. minutum to adapt to the marine environment, including several carbon metabolic pathways, assimilation of various sources of nitrogen and phosphorus. A. minutum was found to encode homologues for several proteins involved in saxitoxin biosynthesis, including the first three proteins in the pathway of saxitoxin biosynthesis, namely sxtA, sxtG and sxtB. The comprehensive transcriptome analysis presented in this study represents a valuable resource for understanding the dinoflagellates molecular metabolic model regarding nutrient acquisition and biosynthesis of saxitoxin.
Plant Resources Utilization among Different Ethnic Groups of Ladakh in Trans-Himalayan Region
Authors: Haq Shiekh Marifatul , Yaqoob Umer , Calixto Eduardo Soares , Rahman Inayat Ur , Hashem Abeer , Abd_Allah Elsayed Fathi , Alakeel Maha Abdullah , Alqarawi Abdulaziz A. , Abdalla Mohnad , Hassan Musheerul , Bussmann Rainer W. , Abbasi Arshad Mehmood , Ur Rahman Sami , Ijaz Farhana
Published: August 2021

The nomadic pastoral indigenous communities of the Ladakhi people share roots with Tibetan culture in terms of food, clothing, religion, festivals, and habits, and rely widely on plant resources for survival and livelihood. This survey was conducted during 2019–2021 to document the indigenous knowledge about plant resources of the Balti, Beda, and Brokpa communities of the Ladakh region, trans-Himalayas. Open- and close-ended semi-structured interviews (N = 184) and group discussions (N = 17) were used to collect the data. Quantitative data was further analyzed using various statistical tools. A total of 105 plant species belonging to 82 genera and 39 families were used as medicine, fuel wood, fragrance, oil, food, flavor, fodder, decoration, and dye. Among these, medicinal use was most prevalent, with 70% of use reports, followed by fodder and fuel wood. Leaves (27%) were the most preferred plant part used, followed by roots and flowers. The principal component analysis revealed five clusters of ethnobotanical usage, i.e., food, medicine, fuel wood, fodder, and fragrance, oil, dye, and flavor. The maximum number of plant species used was reported by the Brokpa, while the Beda reported the minimum number of plant species uses. Delphinium brunonianum, Waldheimia tomentosa, and Juniperus indica played a significant role in the cultural and religious ritual aspects, whereas Allium przewalskianum, Waldheimia tomentosa, Juniperus indica, and Hippophae rhamnoides were commonly used as a livelihood source among Ladakhi communities. The local people collected most plants (65%) for self-consumption, while the rest (35%) were sold in markets as a source of income. The sustainable utilization and management of plant resources by local people is a strategy to boost livelihoods and food security and alleviate poverty.
Mutations of SARS-CoV-2 RBD May Alter Its Molecular Structure to Improve Its Infection Efficiency
Authors: Alaofi Ahmed L. , Shahid Mudassar
Published: August 2021

The receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates the viral–host interaction and is a target for most neutralizing antibodies. Nevertheless, SARS-CoV-2 RBD mutations pose a threat due to their role in host cell entry via the human angiotensin-converting enzyme 2 receptor that might strengthen SARS-CoV-2 infectivity, viral load, or resistance against neutralizing antibodies. To understand the molecular structural link between RBD mutations and infectivity, the top five mutant RBDs (i.e., N501Y, E484K L452R, S477N, and N439K) were selected based on their recorded case numbers. These mutants along with wild-type (WT) RBD were studied through all-atom molecular dynamics (MD) simulations of 100 ns. The principal component analysis and the free energy landscape were used too. Interestingly, N501Y, N439K, and E484K mutations were observed to increase the rigidity in some RBD regions while increasing the flexibility of the receptor-binding motif (RBM) region, suggesting a compensation of the entropy penalty. However, S477N and L452R RBDs were observed to increase the flexibility of the RBM region while maintaining similar flexibility in other RBD regions in comparison to WT RBD. Therefore, both mutations (especially S477N) might destabilize the RBD structure, as loose conformation compactness was observed. The destabilizing effect of S477N RBD was consistent with previous work on S477N mutation. Finally, the free energy landscape results showed that mutations changed WT RBD conformation while local minima were maintained for all mutant RBDs. In conclusion, RBD mutations definitely impact the WT RBD structure and conformation as well as increase the binding affinity to angiotensin-converting enzyme receptor.
Reactive Astrocytosis in a Mouse Model of Chronic Polyamine Catabolism Activation
Authors: Cervetto Chiara , Averna Monica , Vergani Laura , Pedrazzi Marco , Amato Sarah , Pelassa Simone , Giuliani Stefano , Baldini Francesca , Maura Guido , Mariottini Paolo , Marcoli Manuela , Cervelli Manuela
Published: August 2021

Background: In the brain, polyamines are mainly synthesized in neurons, but preferentially accumulated in astrocytes, and are proposed to be involved in neurodegenerative/neuroinflammatory disorders and neuron injury. A transgenic mouse overexpressing spermine oxidase (SMOX, which specifically oxidizes spermine) in the neocortex neurons (Dach-SMOX mouse) was proved to be a model of increased susceptibility to excitotoxic injury. Methods: To investigate possible alterations in synapse functioning in Dach-SMOX mouse, both cerebrocortical nerve terminals (synaptosomes) and astrocytic processes (gliosomes) were analysed by assessing polyamine levels, ezrin and vimentin content, glutamate AMPA receptor activation, calcium influx, and catalase activity. Results: The main findings are as follows: (i) the presence of functional calcium-permeable AMPA receptors in synaptosomes from both control and Dach-SMOX mice, and in gliosomes from Dach-SMOX mice only; (ii) reduced content of spermine in gliosomes from Dach-SMOX mice; and (iii) down-regulation and up-regulation of catalase activity in synaptosomes and gliosomes, respectively, from Dach-SMOX mice. Conclusions: Chronic activation of SMOX in neurons leads to major changes in the astrocyte processes including reduced spermine levels, increased calcium influx through calcium-permeable AMPA receptors, and stimulation of catalase activity. Astrocytosis and the astrocyte process alterations, depending on chronic activation of polyamine catabolism, result in synapse dysregulation and neuronal suffering.
Heart Organoids and Engineered Heart Tissues: Novel Tools for Modeling Human Cardiac Biology and Disease
Authors: Lewis-Israeli Yonatan R. , Wasserman Aaron H. , Aguirre Aitor
Published: August 2021

Organoids are three-dimensional in vitro cell constructs that recapitulate organ properties and structure to a significant extent. They constitute particularly useful models to study unapproachable states in humans, such as embryonic and fetal development, or early disease progression in adults. In recent years organoids have been implemented to model a wide range of different organs and disease conditions. However, the technology for their fabrication and application to cardiovascular studies has been lagging significantly when compared to other organoid types (e.g., brain, pancreas, kidney, intestine). This is a surprising fact since cardiovascular disease (CVD) and congenital heart disease (CHD) constitute the leading cause of mortality and morbidity in the developed world, and the most common birth defect in humans, respectively, and collectively constitute one of the largest unmet medical needs in the modern world. There is a critical need to establish in vitro models of the human heart that faithfully recapitulate its biology and function, thus enabling basic and translational studies to develop new therapeutics. Generating heart organoids that truly resemble the heart has proven difficult due to its complexity, but significant progress has been made recently to overcome this obstacle. In this review, we will discuss progress in novel heart organoid generation methods, the advantages and disadvantages of each approach, and their translational applications for advancing cardiovascular studies and the treatment of heart disorders.
The Role of Polymorphisms in Collagen-Encoding Genes in Intervertebral Disc Degeneration
Authors: Trefilova Vera V. , Shnayder Natalia A. , Petrova Marina M. , Kaskaeva Daria S. , Tutynina Olga V. , Petrov Kirill V. , Popova Tatiana E. , Balberova Olga V. , Medvedev German V. , Nasyrova Regina F.
Published: August 2021

(1) Background: The purpose of this review is to analyze domestic and foreign studies on the role of collagen-encoding genes polymorphism in the development of intervertebral discs (IVDs) degeneration in humans. (2) Methods: We have carried out a search for full-text articles published in e-Library, PubMed, Oxford Press, Clinical Case, Springer, Elsevier and Google Scholar databases. The search was carried out using keywords and their combinations. The search depth was 5 years (2016–2021). In addition, this review includes articles of historical interest. Despite an extensive search, it is possible that we might have missed some studies published in recent years. (3) Results: According to the data of genome-wide and associative genetic studies, the following candidate genes that play a role in the biology of IVDs and the genetic basis of the processes of collagen degeneration of the annulus fibrosus and nucleus pulposus of IVDs in humans are of the greatest interest to researchers: COL1A1, COL2A1, COL9A2, COL9A3, COL11A1 and COL11A2. In addition, the role of genes COL1A2, COL9A1 and others is being actively studied. (4) Conclusions: In our review, we summarized and systematized the available information on the role of genetic factors in IVD collagen fibers turnover and also focused on the functions of different types of collagen present in the IVD. Understanding the etiology of impaired collagen formation can allow doctors to prescribe pathogenetically-based treatment, achieving the most effective results.
Serotonin Transporter Gene Polymorphisms and Maternal Overprotection Regulate Adult Social Expectations on Close Relationships
Authors: Bonassi Andrea , Cataldo Ilaria , Gabrieli Giulio , Lepri Bruno , Esposito Gianluca
Published: August 2021

Humans are evolutionary-driven to adult mating and conceive social expectations on the quality of their affiliations. The genetic susceptibility to adverse environments in critical periods can alter close relationships. The current research investigates how the promoter region of the Serotonin Transporter Gene (5-HTTLPR) and perceived caregiving behavior in childhood could influence the social expectations on close adult relationships. For this purpose, 5-HTTLPR data was collected from the buccal mucosa of 65 Italian individuals (33 males). The participants filled (a) the Parental Bonding Instrument (PBI) to provide the levels of care and overprotection from mother and father, and (b) the Experience in Close Relationships-Revised (ECR-R) to report the social expectations on the intimate relationship assessed in terms of anxiety and avoidance from the partner. An interaction effect between 5-HTTLPR and PBI dimensions on the ECR-R scores was hypothesized. Results confirmed that the interplay between the genetic groups and history of maternal overprotection predicted avoidance experienced in romantic relationships in adulthood. Moreover, both adult anxiety and avoidance felt in an intimate relationship were found to covary as a function of maternal overprotection. The present work proposes further evidence of the genetic and parental mechanisms regulating social expectations involved in close relationships.
Pomegranate Juice Ameliorates Dopamine Release and Behavioral Deficits in a Rat Model of Parkinson’s Disease
Authors: Kujawska Małgorzata , Jourdes Michael , Witucki Łukasz , Karaźniewicz-Łada Marta , Szulc Michał , Górska Agata , Mikołajczak Przemysław Ł. , Teissedre Pierre-Louis , Jodynis-Liebert Jadwiga
Published: August 2021

Pomegranate juice (PJ) is a rich source of ellagitannins (ETs), precursors of colonic metabolite urolithin A, which are believed to contribute to pomegranate’s neuroprotective effect. While many experimental studies involving PJ’s role in Alzheimer’s disease and hypoxic-ischemic brain injury have been conducted, our knowledge of pomegranate’s effects against Parkinson’s disease (PD) is very limited. Previously, we have reported that PJ treatment improved postural stability, which correlated well with enhancement of neuronal survival, protection against oxidative damage, and α-synuclein aggregation. Since olfactory and motor deficits are typical symptoms of PD, in this study, we aimed to investigate the capability of PJ to protect against olfactory, motoric, and neurochemical alterations. To evaluate its efficiency, Wistar rats were given a combined treatment with ROT (1.3 mg/kg b.w./day, s.c.) and PJ (500 mg/kg/day, p.o.) for 35 days. After this, we assessed the olfactory discrimination index (DI) and vertical and horizontal activities as well as levels of dopamine and its main metabolite 3,4-Dihydroxyphenylacetic acid (DOPAC) in the dissected midbrain of animals. Our findings provide the first evidence that PJ treatment protects against ROT-induced DA depletion in the midbrain, which correlates well with improved olfactory function and vertical activity as well as with the presence of urolithin A in the brain.
Brain–Immune Interactions as the Basis of Gulf War Illness: Clinical Assessment and Deployment Profile of 1990–1991 Gulf War Veterans in the Gulf War Illness Consortium (GWIC) Multisite Case-Control Study
Authors: Steele Lea , Klimas Nancy , Krengel Maxine , Quinn Emily , Toomey Rosemary , Little Deborah , Abreu Maria , Aenlle Kristina , Killiany Ronald , Koo Bang-Bon , Janulewicz Patricia , Heeren Timothy , Clark Allison N. , Ajama Joy , Cirillo Joanna , Buentello Gerardo , Lerma Vanesa , Coller Janet K. , Sullivan Kimberly
Published: August 2021

The Boston University-based Gulf War Illness Consortium (GWIC) is a multidisciplinary initiative developed to provide detailed understanding of brain and immune alterations that underlie Gulf War illness (GWI), the persistent multisymptom disorder associated with military service in the 1990–1991 Gulf War. The core GWIC case-control clinical study conducted in-depth brain and immune evaluation of 269 Gulf War veterans (223 GWI cases, 46 controls) at three U.S. sites that included clinical assessments, brain imaging, neuropsychological testing, and analyses of a broad range of immune and immunogenetic parameters. GWI cases were similar to controls on most demographic, military, and deployment characteristics although on average were two years younger, with a higher proportion of enlisted personnel vs. officers. Results of physical evaluation and routine clinical lab tests were largely normal, with few differences between GWI cases and healthy controls. However, veterans with GWI scored significantly worse than controls on standardized assessments of general health, pain, fatigue, and sleep quality and had higher rates of diagnosed conditions that included hypertension, respiratory and sinus conditions, gastrointestinal conditions, and current or lifetime depression and post-traumatic stress disorder. Among multiple deployment experiences/exposures reported by veterans, multivariable logistic regression identified just two significant GWI risk factors: extended use of skin pesticides in theater (adjusted OR = 3.25, p = 0.005) and experiencing mild traumatic brain injury during deployment (OR = 7.39, p = 0.009). Gulf War experiences associated with intense stress or trauma (e.g., participation in ground combat) were not associated with GWI. Data and samples from the GWIC project are now stored in a repository for use by GWI researchers. Future reports will present detailed findings on brain structure and function, immune function, and association of neuroimmune measures with characteristics of GWI and Gulf War service.