bluuebunny/crossref_metadata_2025 huggingface dataset:
Community perception and utilization of raru in Bonalumban Village
Authors: Rangkuti A B , Susilowati A , elfiati D , Zaitunah A , Samsuri  , Iswanto A H , Rambey R , Sucipto T , Hakim L , Azhar I , Syahputra O K H , Harahap M M , Ulfa M , Manurung H , Arinah H , Kusuma Y S , Kholibrina C R , Siregar H , Rangkuti R R
Published: December 2021

Village life around the forest is inseparable from the existence of the forest. The use of raru as a forest product has been passed down from generation to generation in the community of Bonalumban Village, Tukka District, Central Tapanuli Regency, North Sumatra. The study of community perceptions and dependencies on Raru in Bonalumban village is needed to determine the potential of raru and encourage efforts to preserve raru. The method of selecting respondents is done by purposive sampling by selecting general respondents and specialized respondents. Data analysis uses descriptive statistics. Public perceptions about the existence of raru in nature are still plentiful so there is no public effort to regenerate. Community dependency on raru is grouped into four categories, that is for sale, palm wine concoctions, animal cages, and building materials. Raru harvesting activities by felling without being accompanied by cultivation activities causes the presence of raru vegetation to decrease.
Squalene decreased fasting blood glucose level of type ii diabetic rats
Authors: Widyawati T , Syarifah S , Sumantri I B
Published: December 2021

Squaleneis a chemical compound that has been reported to have antidiabetic activity. The present study aimed to investigate the effect of squalene on fasting blood glucose level (FBGL) in type 2 diabetic rats. Diabetes type II in rats was obtained by giving nicotinamide (120 mg/kg) before high dose streptozotocin (60 mg/kg) intraperitoneally. A total of 18 diabetic rats were divided into 3 groups and served once daily for 12 days as follows; Group I Aquades (Diabetic Control) 10 ml/kg, Group II (Metformin 45 mg/kg) and Group III (Squalene 160 mg/kg). FBGL was measured at day 0, day 6 and day 12. The results showed that FBGL in both Squalene- (194.67 ± 28.32 mg/dL) and Metformin- (178.50 ± 34.27 mg/dL) were significantly decreased after 12 days intervention compared to Diabetic Control-treated groups (438.33 ± 65.79 µmol/L) with p<0.001. This study concluded that squalene was able to decrease FBGL in type II diabetic rats.
Eucalyptus plantation and its integrated supply chain in pulp and paper mill
Authors: Sembiring N , Napitupulu H L , Sembiring M T , Sipahutar A I , Tarigan C A
Published: December 2021

Nowadays, demand of the pulp and paper production increases quickly. That increasing demand has led to demanding of eucalyptus plantation. That is why eucalyptus plantation has rapidly expanded and became the most planted tree species across the world over the last century. Not only because its unique characteristics, increasing of eucalyptus plantation also happened because its growing gives a socio-economic benefits, industrial benefits, and environmental impacts. However, increasing demand of eucalyptus certainly make availability of eucalyptus being determined, known as limited supply chain. Due to the increasing demand and limited supply chain, the eucalyptus wood supply should managed, by integrated the supply chain well. In this research study, the supply chain of eucalyptus wood for pulp and paper industry was diagnosed and divided into three degree, there are direct supply chain, extended supply chain, and ultimate supply chain. Four main stakeholders in this chain identified consisted of the eucalyptus seedling developers and sellers, the suppliers, the distribution centers, and the pulp and paper production plants.
Addition of divinyl benzene comonomer to glycidyl methacrylate grafting to cyclic natural rubber
Authors: Alfian Z , Tamrin  , Eddiyanto  , Nasution N
Published: December 2021

The process of thermally grafting glycidyl methacrylate (GMA) on cyclic natural rubber (CNR) compared to the addition of an initiator of organic peroxide, dicumyl peroxide (DCP) and using cross-linker divinyl benzene (DVB) has been carried out by means of melt processing. The main aims of the modified GMA grafted to CNR was to increase the polarity of the polymer to be used as a compatibiliser agent in asphalt modification. The addition of DVB comonomer in the processing was to increase the amount of GMA implanted in cyclic rubber as measured by the degree of GMA grafting. The grafting method was carried out by melting polymer (melt processing) at high temperatures in the reactor internal mixer (Brabender model). The grafting reaction took place at a temperature of 160°C, 60 rpm rotor rotation for 10 minutes of mixing. To determine the GMA grafting reaction on cyclic rubber, characterization was carried out with Fourier Transformed Infra Red (FT-IR) while the degree of GMA grafting on natural rubber was determined by acid-base titration method in organic solvents. It was found that the GMA grafting process on cyclic natural rubber could easily occur in the melting phase at high temperatures and increase with the addition of dicumyl peroxide (DCP) peroxide. Although the addition of divinyl benzene (DVB) comonomer can increase the degree of grafting of GMA on CNR, the addition of comonomer can cause high cross-linking.
Genetic sequence analysis of Arthrobotrys thaumasia DS01 (Monacrosporium thaumasium): A new report from North Sumatra, Indonesia
Authors: Hastuti L D S , Berliani K , Mulya M B , Hartanto A , Pahlevi S
Published: December 2021

Exploration of nematode-trapping fungi (NTF) has been conducted previously in urban area, the Medan city, North Sumatra, Indonesia. The survey reported a number of NTF species inhabiting soil samples of organic wastes and decaying litter. Upon finding a suitable NTF isolate as nematode biocontrol agent, species identification is currently carried out based on molecular evidences. One morphotype named isolate DS01 is suspected as member of Arthrobotrys based on morphological characteristics. Molecular sequencing on rDNA or ITS-DNA region has successfully been performed. The genetic sequence was analyzed with database retrieved in NCBI, revealing the identity of A. thaumasia/M. thamasium. Based on recent findings, this is the first report on indigenous strain of A. thaumasia from Indonesia.
Gut content analysis of cattle egrets (Bubulcus ibis) in Asam Kumbang Crocodile Park, North Sumatra
Authors: Jumilawaty E , Namira N , Anggelicha A , Hartanto A
Published: December 2021

Cattle egret (Bubulcus ibis) is a widespread avian species inhabiting a variety of natural and artificial sites. Asam Kumbang Crocodile Park is one of establishments that occupied some portions of B. ibis habitat with no recent information on the food resource and prey availability for the viability of B. ibis. To this aim, we collected 10 random individuals of B. ibis to be sacrificed for gut content analysis of prey species. A total of 19 taxa was found as prey items in the gut of B. ibis with the dominant group from insects (Orthoptera, Scolopendromorpha). The diversity of prey species was categorized as moderate with intraspecific diet variation which formed four guilds that fed on specific taxa namely Group 1 (Haplotaxida, Lepidoptera, Anurans), Group 2 (Blattodea, Coleoptera, Araneae), Group 3 (Orthoptera, Scolopendromorpha); and Group 4 (Dermaptera, Squamata). Oxya chinensis and Oxya velox are important rice grasshopper pests that were also found in the gut of B. ibis reflecting their potential function as biological control agent.
Economic feasibility of obtaining wood phytomass through accelerated cultivation of pine wood on postagrogenic lands
Authors: Yanush Sergey , Danilov Dmitry , Kharlanov Aleksei
Published: December 2021

The paper examines economic efficiency of obtaining wood biomass through accelerated cultivation of pine on postagrogenic lands of the Leningrad Region of Northwest Russia. In experimental pine plantations, we selected model trees and calculated the stem wood biomass of 11-year old stands with different stem densities. Assessments of the economic efficiency of the production of wood chips from pine wood grown on postagrogenic lands were based on a set of machines and mechanisms, consumables and wages. A break-even point was determined for the production of wood chips in plantations with different stem densities. In the variant with a stand density of 2900 trees per hectare, it is possible to make a profit, because a break-even point was obtained for the investments made. Pine stands of such density can be taken as a prototype, when creating and growing short rotation plantations. Based on the results of the study, it is possible to predict a further increase in pine wood biomass in a plantation on post-agrogenic soils. On postagrogenic lands, marketable wood pulp can be obtained within a short period of time; thus such lands will be involved in an economic turnover.
Fiber-optic light guides as secondary sources of light for hen keeping in cage batteries
Authors: Gladin Dmitry , Kavtarashvili Alexey
Published: December 2021

The method of local illumination of cage batteries with LED lamps, previously developed by us, made it possible to increase the livability and productivity of hens in comparison with the traditional method. In this case, the placement of low-power LED sources directly in each cage requires a large number of rather complex electronic devices across the whole metal structure of the cage battery and extended power transmission lines. This work compares the efficiency of local LED lighting and fiber-optic light guides when keeping hens of the productive flocks of cross SP-789 in traditional cages. The 120-day-old hens were divided by the method of analogs in 2 groups of 100 heads. In the control group, LED lamps were used for local illumination of hens, and in the experimental group, fiber-optic light guides were used with LEDs as the primary source of illumination. The color temperature of light-emitting diodes was 3000 K. Up to 260 days of age, hens were kept in triple-tiered cage batteries, 5 animals per cage. The results of the study showed that in the control and experimental groups, the livability of the livestock was 95.0 and 99.0%, the egg production per initial hen was 99.2 and 102.5 pieces. The weight of eggs was 57.9 and 58.3 g, the yield of the egg weight per initial hen was 5.81 and 5.98 kg, feed consumption for 10 eggs and 1 kg of egg weight were 1.37, 2.35 and 1.30, 2.23 kg, respectively, without significant changes in the morphological and chemical qualities of eggs.
TECHNOLOGICAL AND ECONOMIC ASPECTS OF INDUSTRIAL PRODUCTION OF BROILER MEAT
Authors: Buyarov Viktor , Mednova Valentina , Buyarov Aleksandr , Andreeva Olga
Published: December 2021

The issues of development and implementation of resource-saving technologies in broiler poultry farming have a complex purposefulness and their solution must be scientifically substantiated and cost-effective. The productive qualities of meat chickens and broiler chickens of the productive crosses are significantly influenced by many factors that make up the conditions of keeping and feeding. The use of Apex and Emicidin preparations both separately and in combination with each other when rearing chickens of the broiler parent flock of the Ross 308 cross had a positive effect on their productivity and the quality of hatching eggs obtained at the end of the productive period, at the age of 52 weeks. Egg production per initial hen exceeded that in the control group by 2.6% in experimental group 1 (Emicidin), by 3.2% in experimental group 2 (Apex 3010), and by 5.8% in experimental group 3 (combined use of Emicidin and Apex 3010). The determined zootechnical indicators showed high efficiency of the use of the GerbaStor preparation for the floor rearing of broiler chickens of the Ross-308 cross in conditions of increased stocking density in winter. The difference in the productivity index was 18 units, and in meat yield from 1 m² of floor, it was 1.37 kg (3.8%) in favor of the broilers of the experimental group 2, who received GerbaStor. The profitability of the production and sale of meat of broilers grown under an increased stocking density using the GerbaStor preparation was 3.95% higher than that of broilers in the control group.
The influence of cultivation of drone larvae on honey productivity of bee colonies
Authors: Yankina Olga L. , Prikhodko Anna N. , Kim Natalia A. , Skolov Andrey A. , Podvalova Victoriya V.
Published: December 2021

Homogenate is a source of a huge amount of biologically active substances. However, it is produced in a small number of apiaries, since the cultivation of drone brood negatively affects honey productivity. This paper aimed to study the influence of rearing drone larvae on the power and honey productivity of bee colonies since these issues are not widely addressed in scientific literature. Three groups (control, experimental 1, and experimental 2) of 10 bee colonies were formed by the method of pairs-analogs. One brood frame was placed in the bee colonies of the first experimental group, and two frames were placed in the colonies of the second experimental group. The brood frames were placed on June 12, the clipping was done on June 30, and the honey was collected on July 12. The power of colonies was taken into account after wintering (in March) and at the end of the pre-wintering season (in November). As a result of the research, it was revealed that the homogenate of drone brood can be obtained in apiaries of the Primorsky Krai. It was proved that when adding one brood frame to obtain a homogenate, the main production of the apiary - honey, does not decrease, and a negative effect on honey productivity was observed when adding the second brood frame.
Genetic diversity of Kemerovo pig breed based on microsatellite markers
Authors: Chydym S M , Kochneva M L , Vilgelmi I A , Zhychaev K V
Published: December 2021

Today, the urgent task is to preserve the gene pool of local and regional breeds with their special and useful traits. These features allow them to represent an additional reservoir of genetic diversity. Analysis of the genetic diversity of three pig populations (Kemerovo pig breed, Kemerovo × Landrace crossbreds and Kemerovo × Landrace × Pietrain crossbreds) by microsatellites showed that significant allelic diversity is observed for some loci, while for others it is low. The highest number of alleles was in the purebred pigs in comparison with crossbreds. The greatest range of alleles per locus was noted in the three-breed cross pigs. The most polymorphic locus in all populations was SO005, and the least polymorphic was SW72. It was noted that for most loci the observed heterozygosity corresponds to the expected one, while for others a shortage of heterozygotes is detected.
DEVELOPMENT OF THE BLACK SEA HYDROBIONT RESOURCE STUDY METHOD AS A NEW ECONOMICALLY FEASIBLE DIRECTION OF PHARMACOLOGY AIMING TOWARD SAVE HUMAN HEALTH AND ECOLOGY
Authors: Chzhu O P , Araviashvili D E , Tumanova A L
Published: December 2021

The selection of the Black Sea aquatic area as a hydrobiont biologically active compound source allows resolving several environmental problems including development of an integrated hydrobiological resource management system for the Black Sea as a new sector of modern pharmacology. Aquatic organisms produce unique secondary metabolites. This paper presents the results of studies on the development of the biologically active substance extraction technology from non-commercial aquatic organisms as well as a preliminary assessment of the biochemical activity of the substrates obtained. Biologically active substances were extracted using the of two-phase extraction method in conjunction with ultrasound. For the substrates, the antioxidant activity was determined utilising the method that allow conducting screening of pharmaceutical raw materials and biologically active substances with high antioxidant activity. The protective activity evaluation was carried out during the study of the enzymatic alcoholic fermentation kinetics within a self-contained system. The evaluation of lymphocytes proliferative activities influenced by the obtained substrates was carried out using the cultivation method. The acquired data allows recommending the two-phase extraction method combined with ultrasonic voiceover as the effective one when processing analogical marine raw materials. The isolated substrates are characterised by a pronounced biochemical activity in relation to the living systems cells, which suggests a significant synergistic effect with derivatives of the microalgae Chlorella Vulgaris.
Mobilization hard-to-reach phosphorus compounds in soil and influence growth processes of microbiological association
Authors: Rjevskaya Viktoriya , Omelchenko Aleksandr , Semenova Elena , Teplitskaya Ludmila , Tsokalo Inna
Published: December 2021

The article presents results of research of monocultures and association of lactic-oxide bacteria, purple nonsulfur bacteria and yeast on indicators of stimulation of growth processes, nitrogen fixation and phosphate mobilization for 2 crops: one-year and longterm. The research was carried out in both laboratory and production conditions. It is shown that milk-oxide and purple nonsulfur bacteria stimulate growth processes of wheat variety Nador in aquatic culture by 20-30%, and their consortium with yeast - by 30%. In comparison with control, an increase of 3.4 t/ha in the yield of Golden Delishes apples was detected with 2fold treatment based on a consortium of lactic oxide, purple nonsulfur bacteria and sucromycetes. It was determined that all the strains of the above-mentioned groups of microorganisms studied mobilized hard-to-soluble compounds of phosphorus, and only purple nonsulfur bacteria were capable of nitrogen fixation.
Biotechnology methods to produce planting material of the genus Rubus L.
Authors: Raeva-Bogoslovskaya E N , Molkanova O I , Krakhmaleva I L , Soboleva E V
Published: December 2021

The work is devoted to improving clonal micropropagation methods of the genus Rubus representatives. When cultivating R. arcticus L. cultivars, the optimal concentration of 6-benzylaminopurine (6-BAP) was 0.3 mg L−1. Significant effect of FeEDTA concentration in the nutrient medium on multiplication of R. idaeus L. cultivars was established. The interaction of auxin type and plant genotype was revealed during rooting of blackberry cultivars and raspberry-blackberry hybrids. The nutrient medium supplemented with 0.5 mg L-1 indoleacetic acid contributed to the highest percentage of rhizogenesis in R. arcticus representatives (82%). The optimal growth regulator for conservation of raspberry explants was 6-BAP at a concentration of 0.3 mg L-1. One of the most representative in vitro collections of Rubus cultivars has been created.
Assessment of resistance of potato hybrids from the Russian Potato Research Center’s collection to Globodera rostochiensis
Authors: Koroleva A K , Sivolapova A B , Voinova M S , Ivanova A S , Meleshin A A , Manankov V V
Published: December 2021

The potato cyst nematodes (PCN) Globodera rostochiensis and Globodera pallida are the most common pests feeding on potato roots. These pests have an aggressive distribution dynamic and bring a lot of damage to yield. The study presents results of comprehensively test new potato genotypes for resistance to the golden potato nematode (Globodera rostochiensis), for its further use in breeding and the creation of economically valuable resistant varieties. Almost half of the samples showed laboratory resistance to golden potato nematode, the rest were susceptible. 26 samples have shown the presence of two molecular markers (N146, N19), indicating the presence of H1 gene. Almost all samples showed a correspondence between laboratory resistance and the presence of H1 gene markers.
Microbiomes of natural and abandoned agricultural soils of the Central part of Yamal region
Authors: Abakumov E , Kimeklis A , Gladkov G , Andronov E , Morgun E
Published: December 2021

Soil cover of the northern most regions of Eurasia are considered as underestimated in terms of their possible role in expansions of current agriculture to the cryolithozone. In this context, abandoned agricultural soils of Yamal region were investigated in terms of morphology, chemistry and taxonomy microbiome compositions and compared in these terms with mature tundra and taiga soils of pristine environments. The level of soil fertility was low in all cases – former agricultural soils and pristine ones. The level of microorganism’s biodiversity was higher in soils of agricultural lands. This fact indicates that the agricultural soil treatment in polar terrestrial ecosystem results in increasing of soil microbial biodiversity due to diversification of ecological niches. Also the is an essential lack of nitrogen sources in all permafrost affected soils studied.
Comparative study of photosynthetic pigments in the leaves of forage plants of the cryolithozone of Yakutia
Authors: Nokhsorov V V , Petrov K A
Published: December 2021

Thin-layer chromatography and spectrophotometry were used to determine the content of photosynthetic pigments (chlorophylls a and b, carotenoids) in green leaves of forage plants. It was found that the content of carotenoids was high for forage grasses with a high content of green pigments. It was revealed that β-carotene plays the most important role since it is a precursor of vitamin A and lutein, which has a high antioxidant (protective) property. According to modern concepts, lutein and β-carotene actively function in various organs of herbivores. They play a special role in the functioning of the mammalian visual apparatus, protecting this organ from the light factor. In addition, lutein is one of the physiologically active substances that make up cow’s milk.
Effect of potassium humate and growth stage on phenolic compound and vitamin C accumulation in kale (Brassica oleracea var. sabellica)
Authors: Anteh J D , Timofeeva O A , Mostyakova A A
Published: December 2021

Kale is one of the top economically valuable crops in the world because of its high antioxidant content. Research shows that the antioxidant profile of Brassica crops varies with growth stages due to soil fertility, temperature, light and other agronomic factors. This study aimed to analyze the effect of potassium humate on phenolic compound content, the greatest contributor to the antioxidant properties of the highest-ranking superfood kale (Brassica oleracea var. sabellica). Our results showed that potassium humate at different growth stages elicited phenolic compounds in the studied samples. Leaves of 22 weeks old plants accumulated phenolic compounds about two times higher than those of 7 weeks old plants. Vitamin C content was increased by potassium humate treatment at 7 weeks. At 22 weeks levels in controls and treated kale leaves did not significantly differ.
RNA-Seq of potato plants reveals a complex of new latent bacterial plant pathogens
Authors: Ignatov A N , Semenov A , Tešić S , Kyrova E , Goryunova S
Published: December 2021

The throughput and single-base resolution of RNA-Sequencing (RNA-Seq) have contributed to a dramatic change in diagnostics of viruses and other plant pathogens. A transcriptome represents all RNA molecules, including the coding mRNAs as well as the noncoding rRNA, tRNA, etc. A distinct advantage of RNA-Seq is that cDNA fragments are directly sequenced and the reads can be compared to available reference genome sequences. This approach allows the simultaneous and hypothesis-free identification of all pathogens in the plant. We conducted surveys for potato (Solanum tuberosum L.) -associated phytopathogenic bacteria in 56 original and GenBank RNA-seq data sets for potato breeding material. Bacteria of genera Pseudomonas, Burkholderia, Ralstonia, Xanthomonas, Agrobacterium, and species of family Enterobacteriaceae were most frequently detected in RNA sets from the studied plants. RNA-seq reads identified as Xanthomonas spp. were within X. vesicatoria, and some other species. Xanthomonas spp. covered up to 9,1% of all reads and included the major clades of these bacteria known as pathogens of solanaceous crops, but potato. Bacteria of genus Xanthomonas infect different plant species under artificial inoculation, suggesting that they are shared among wild plants and crops. Our studies indicated that a larger number of solanaceous plants can be occupied by specific Xanthomonas pathovars as endophytes or latent pathogens. Revealing bacteria distribution in the plant breeding material using RNA-seq data improves our knowledge on the ecology of plant pathogens.
Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrity
Authors: Xu Weiyi , Alpha Kyle M. , Zehrbach Nicholas M. , Turner Christopher E.
Published: December 2021

Paxillin is shown to be critical for collective breast cancer cell invasion and metastasis via maintenance of adherens junctions (AJs). Mechanistically, paxillin-dependent E-cadherin endocytosis and recycling, microtubule acetylation, and modulation of Rho GTPase activities together contribute to AJ integrity.
A Dyadic Investigation of Depressed Affect and Interspousal Behavior in Couples With Chronic Back Pain
Authors: Post Kristina M , Smith David A , Burns John W , Porter Laura S , Keefe Francis J
Published: December 2021

Background

Depression and marital discord are characteristic not only of individuals with chronic low back pain (ICPs) but also of their spouses.

Purpose

We examined actor–partner interdependence models to evaluate associations among depressed affect and criticism and support of partners at the same time point (concurrent effects) and 3 hr later (lagged effects). Fully dyadic models were used to account for both within-person and cross-spouse associations among depressed affect, criticism, and support for ICPs and spouses. We also examined the direction of the relationships (depressed affect predicting behavior and behavior predicting depressed affect) all while controlling for pain intensity, pain behavior, and the prior dependent variable.

Methods

ICPs (n = 105) and their spouses completed electronic diary measures of depressed affect and behavior (criticism and support) five times a day for 2 weeks. Hierarchical linear modeling with person-mean centering was used for data analysis.

Results

Within the same 3 hr epoch, more depressed affect was related to higher criticism and generally less support. Lagged analyses suggested bidirectional relationships between spouse’s own depressed affect and spouse’s own criticism of ICPs. Spouse depressed affect was also associated with decreased support received from ICPs. Pain behavior and pain intensity were also related to depressed affect, criticism, and support especially concurrently.

Conclusions

Theories and interventions need to address not only ICP depressed affect but also spouse depressed affect, as spouse depressed affect may be a stress generating precursor to criticism and support.
Testicular Lesions in Infertile Men
Authors: Dupeux Margot , Maxwell Florian , Rocher Laurence , Izard Vincent , Guettier Catherine , Ferlicot Sophie
Published: December 2021

Objectives

An increasing number of incidental testicular tumors are diagnosed in patients during infertility workup. The aim of this study was to evaluate the accuracy of frozen section examination (FSE) for the management of these tumors.

Methods

We retrospectively studied a series of 46 testicular tumors diagnosed during exploration for infertility from 2000 to 2019 and submitted for FSE.

Results

A diagnosis of malignancy was made in 23 cases on both gross examination (yellow-white or cream-colored nodules for seminomas) and FSE, then confirmed on final diagnosis in 22 of the cases. One seminoma reported on FSE was revised as being a Leydig cell tumor. The 23 other lesions were diagnosed as benign on FSE, including 11 Leydig cell tumors (yellow-brown nodules), 2 Leydig cell hyperplasias, and 10 whitish fibrous lesions. All Leydig cell lesions were confirmed except 1, which was reclassified as a Sertoli cell tumor. Of the 10 cases of fibrous lesions, 6 were associated with malignancy.

Conclusions

The high incidence of Leydig cell tumors and the accuracy of FSE for these lesions demonstrate the interest in FSE. In contrast, FSE is not reliable for fibrous lesions, and surgeons should be aware that a fibrosis result often corresponds with regressed tumors.
Personality: A potentially untapped resource in the selection of postgraduate pharmacy residents
Authors: Reed Brent N , Gale Stormi E , Martinelli Ashley N , Sparkes Tracy M , Tata Asha L , Williams Carla , Yeung Siu Yan A , Armahizer Michael J
Published: December 2021

Purpose

This study assessed whether personality testing of postgraduate year 1 (PGY1) pharmacy residency applicants was feasible and predicted important selection outcomes, including interview offers.

Methods

Applicants to the PGY1 pharmacy residency program at a large academic medical center were invited to complete a 50-item online personality test based on the 5-factor model (ie, the “Big Five”). Scores were sealed until after matching, at which point they were compared to screening, interview, and ranking and match outcomes. Endpoints of interest included the feasibility of the test (eg, time required for completion, completion rate) and whether personality predicted the odds of an interview offer.

Results

The personality test was taken by 137 PGY1 applicants (69.5%) and required a median of 6.8 minutes to complete. Openness to experience was associated with decreased odds of an interview offer (adjusted odds ratio [OR], 0.86; 95% confidence interval [CI], 0.75-0.98), whereas conscientiousness and extraversion were associated with increased odds of an interview offer (conscientiousness: adjusted OR, 1.26; 95% CI, 1.02-1.55; extraversion: OR, 1.16; 95% CI, 1.03-1.31). When combined with traditional screening criteria (eg, awards, leadership positions), openness to experience and extraversion remained predictors of an interview offer (in the directions specified above), whereas conscientiousness did not. In an exploratory analysis of interviewees, agreeableness was a negative predictor of interview score. Personality did not predict screening scores or final ranking.

Conclusion

Personality testing, based on the traits desired at individual residency programs, could be a valuable addition to the methods used for selecting PGY1 pharmacy residents.
P010 FEASIBILITY OF MIDLINE RECONSTRUCTION WITHOUT MYOFASCIAL RELEASE (COMPONENT SEPARATION) AFTER PREOPERATIVE INFILTRATION OF LATERAL ABDOMINAL WALL MUSCULATURE WITH BOTULINUM TOXIN
Authors: Gabersek Ana , Mayer Franz , Mitterwallner Stefan , Kaufmann Reinhard , Bittner Reinhard , Emmanuel Klaus , Lechner Michael
Published: December 2021

Aim

Aim of the analysis was to evaluate whether preoperative botulinum toxin infiltration may facilitate anatomical midline reconstruction without the need for – otherwise pre-operative assumed – surgical component separation.

Material and Methods

Total of 58 patients with complex abdominal wall hernias were included in our single-center retrospective analysis between 03/2015 and 12/2020. Size of the defect, HSV/ACV-ratio, rectus-to-defect-width-ratio (“Carbonell-Index”) as well as risk factors were analyzed. In all patients muscles of the lateral abdominal wall were infiltrated with 300-500 IE of botulinum toxin 4 weeks prior to the abdominal wall reconstruction. CT scans were performed before and 4 weeks after the botulinum toxin infiltration.

Results

Total of 58 patients (M/F-ratio 36:22), with a mean age of 63.8 years were included in our analysis. Mean BMI was 29.5 kg/m². Total of 50 incisional, 3 umbilical, 2 posttraumatic diaphragmatic hernias and 3 scrotal hernias were analyzed. Surgical component separation after the infiltration with botulinum toxin was necessary in 43% of the cases.

Conclusions

Preoperative infiltration of the lateral abdominal wall musculature with botulinum toxin facilitated midline reconstruction of the abdominal wall without the need for myofascial release in 57%. Reduction of surgical trauma could therefore be achieved in several patients.
P014 COMPARATIVE EFFECTIVENESS OF RESORBABLE SYNTHETIC ONLAY AND BIOLOGIC INTRAPERITONEAL MESH FOR ABDOMINAL WALL RECONSTRUCTION: A 2-YEAR MATCHED PAIR ANALYSIS
Authors: Othman Sammy , Christopher Adrienne , Patel Viren , Jia Hanna , Mellia Joseph , Kovach Stephen , Fischer John
Published: December 2021

Aim

The literature currently lacks comparative studies examining the relative effectiveness of anatomic planes and mesh selection when combating abdominal wall reconstruction (AWR), particularly when the retrorectus sublay space is not available. The aim of this study was to examine the efficacy of resorbable synthetic mesh onlay (RSOM) plane against biologic mesh in the intraperionteal plane (BIPM).

Methods

A single center, two surgeon, 5-year retrospective review (2014-2019) was performed examining subjects who underwent AWR in the onlay plane with resorbable synthetic mesh or the intraperitoneal plane with biologic mesh. A matched paired analysis was conducted. Data examining demographic characteristics, intraoperative variables, post-operative outcomes, and costs were analyzed.

Results

A total of 88 subjects (44 per group) were identified (median follow-up: 24.5 months). The mean age was 57.7 years, with a mean BMI of 30.4 kg/m2. The average defect size was 292 ± 237 cm2, with most wounds being clean-contaminated (48.9%), and 55% having prior failed repair. RSOM subjects were significantly less likely (4.5%) to experience recurrence compared to BIPM (22.7%; p<0.026.). Additionally, RSOM suffered less post-operative surgical site occurrences (18.2% vs. 40.9%;p<0.019) and required fewer procedural interventions (11.4% vs. 36.4%;p<0.011). RSOM was also associated with significantly less total costs ($16,658 ± 14,930) compared to BIPM ($27,645 ± 16,864;p<0.001).

Conclusion

When faced with hernia repair, the selection of resorbable synthetic mesh in the onlay plane may be preferable to biologic mesh place in the intraperitoneal plane due to lower long-term recurrence rates, surgical site complications, and costs.
P037 TOTALLY ENDOSCOPIC SUBLAY REPAIR FOR DIASTASIS RECTI AND CONCOMITANT MIDLINE HERNIA: FIRST CASE VIDEO-REPORT
Authors: Bertoglio Camillo Leonardo , Morini Lorenzo , Grimaldi Simona , Carnevali Pietro , Ferrari Giovanni
Published: December 2021

Aim

We describe in detail the step by step technique of the first case of TES repair at our institution.

Methods

We selected the case of a M2W3L3 hernia associated to xipho-umbilical diastasis recti in a young woman symptomatic for a progressively worsening back pain and local bulky sensation.

Results

The intervention is started directly with a suprapubic transverse incision of 2.5 cm and a circumferential, atraumatic retraction is inserted after a small incision of the anterior rectus sheath. Blunt dissection is further continued through this access by luxating the underlying rectus muscles to separate the preperitoneal space below the arcuate line. The pneumo-preperitoneum is then inducted through this port. Laparoscopic dissection allows for enlargement of the avascular space laterally and then two 5-mm trocars are placed on the bilateral aspects of rectus muscles. By means of a lap bipolar dissector the edge of posterior rectus sheath are incised from the arcuate line following the diastasis laterally up to the subxiphoid space. The Rives plane is recovered without opening of the linea alba. After reduction of the M2 hernia both the posterior sheath and the diastatic anterior fascia are sutured with a running long-resorbable 2/0 barbed suture. Polyvinylidene fluoride (PVDF) mesh fixed with the use of an hystoacrilic glue.

Conclusion

Minimally invasive extraperitoneal repair of small/medium hernia defects of the linea alba is gaining wide acceptance. Concomitant presence of rectum diastasis recti seems to be the ideal indication to approach the learning curve of such a technically demanding procedure
P042 CHARACTERIZATION OF INNATE AND ADAPTIVE IMMUNE CELLS INVOLVED IN THE FOREIGN BODY REACTION TO POLYPROPYLENE MESHES IN THE HUMAN ABDOMEN
Authors: Dievernich Axel , Achenbach Pascal , Davies Luke , Klinge Uwe
Published: December 2021

Aim

Polypropylene (PP) mesh is widely used to reinforce tissues. The foreign body reaction (FBR) to the implant is dominated by innate immune cells, especially macrophages. However, considerable numbers of adaptive immune cells have also been regularly observed, which appear to play a crucial role in the long-term host response. This study investigated the FBR to seven human PP meshes, which were removed from the abdomen for recurrence after a median of one year.

Material and Methods

Using immunofluorescence microscopy and distance maps, the FBR was spatially analyzed for various innate (e.g., CD68+ macrophages, CD56+ NK) and adaptive immune cells (CD3+ T, CD4+ T-helper, CD8+ cytotoxic, FoxP3+ T-regulatory, CD20+ B) as well as “conventional” immune cells (defined as cells expressing their specific immune cell marker without co-expressing CD68).

Results

T-helper cells (19%) and regulatory T-cells (25%) were present at comparable rates to macrophages, and clustered significantly toward the mesh fibers. For all cell types the lowest proportions of “conventional” cells (< 60%) were observed at the mesh–tissue interface, but increased considerably at about 50–100 µm, indicating reduced stimulation with rising distance to the mesh fibers.

Conclusions

Both innate and adaptive immune cells participate in the chronic FBR to PP meshes with T cells and macrophages being the predominant cell types, respectively. Furthermore, many cells present a “hybrid” pattern near the mesh fibers. The complexity of the local immune reaction may explain why approaches focusing on specific cell types have not been very successful in reducing the chronic FBR.
P095 ARE POLYPROPYLENE MESH IMPLANTS ASSOCIATED WITH SYSTEMIC AUTOIMMUNE/INFLAMMATORY SYNDROMES?
Authors: Kowalik Claudia , Zwolsman Sandra , Malekzadeh Arjan , Roumen Rudi , Zwaans Willem , Roovers Jan-Paul
Published: December 2021

Aim

Worldwide there is a lot of commotion about the effects and complications of mesh implants. The surgical implantation of polypropylene (PP) meshes has been linked to the occurrence of systemic autoimmune disorders (SAIS). We performed a systematic review to determine whether PP implants for inguinal, ventral hernia or pelvic floor surgery are associated with the development of SAIS.

Material and Methods

We searched Embase, Medline, Web of Science, Scopus, Cochrane library, clinicaltrialsregister.eu, clinicaltrails.gov and WHO-ICTR platform. Forward and backward reference searching was performed to avoid missing relevant papers. All types of studies, except case studies, reporting SAIS in patients having a PP implant for either pelvic floor surgery, ventral or inguinal hernia repair were included. Animal studies were excluded. We intended to perform a meta-analysis. The quality of evidence was assessed with the Newcastle-Ottawa Scale. This study was registered at Prospero (CRD42020220705).

Results

Of 1938 records identified, 3 were eligible. Two studies involving retrospective matched cohorts focused on mesh surgery for vaginal prolapse or inguinal hernia compared to hysterectomy and colonoscopy, respectively. These reports had a low risk of bias. The third study was a case-series with a high risk of bias, with a sample of 714 patients with systemic disease, 40 of them had PP mesh implanted. A meta-analysis showed no association when comparing systemic disease between mesh and control groups. Calculated Risk Ratio was 0.9 (95%CI 0.78-1.04).

Conclusions

To date, there is no evidence to suggest a causal relationship between being implanted with a PP mesh and the occurrence of SAIS.
P122 LAPAROSCOPIC APPROACH TO THE ABDOMINAL WALL WITH CLOSURE OF THE DEFECT, OUR INITIAL RESULTS
Authors: Otegi Ibai , Blazquez Lucas , Rico Aida , Marzo Rocío Ruiz , Sebastian Jose Luis , Uribazo Mauricio , Novo Igor , Aramendia Irene
Published: December 2021

Aim

The aim of the study is to evaluate the closure of the defect in postoperative pain, seroma and recurrence.

Material and Methods

Inclusion criteria were; defect width of 2 - 4 cm and body mass index ≤ 35 kg / m2, or previous intraperitoneal surgery. A prospective database of perioperative variables have been collected. We consider postoperative pain as a value ≥5 on the Visual Analogue Scale, seroma types III and IV of the Morales-Conde classification.

Results

32 patients were operated with laparoscopic closure of the defect (LCD) between 2018 and 2020. The mean age was 59 years and body mass index was 31 kg / m2. 14 patients had incisional hernia. 90% of the cases were M2-3 with a mean defect diameter of 2.7 cm. In 5 cases, a preperitoneal mesh was placed without takers, the mean size of the mesh was 15 cm. The mean duration of the procedures was 96.1 min. Seven (22%) patients presented postoperative pain, of which 5 had 24 h limited and in two cases it lasted up to 48 h. No patient presented pain at the 30-day evaluation. The mean length of stay was 1.8 days. The mean follow-up was 23 months, one seroma (3.1%) and three (9.4%) recurrences were diagnosed.

Conclusions

In our initial experience, we considered LCD a safe technique with benefits for selected patients.
P127 ROBOTIC TRANSABDOMINAL RETRO-RECTUS HERNIA REPAIR VS. LAPAROSCOPIC INTRAPERITONEAL ONLAY MESH REPAIR: IMPROVING POSTOPERATIVE OUTCOMES
Authors: Willaume Mette , Jorgensen Lars Nannestad , Jensen Kristian Kiim
Published: December 2021

Aim

“The optimal repair technique for small and medium-sized ventral hernias in obese patients remains unknown. We aimed to evaluate results after robotic-assisted laparoscopic transabdominal repair with retro-rectus mesh placement (rTARUP) compared with laparoscopic intraperitoneal onlay mesh repair (IPOM).”

Material and Methods

“Retrospective cohort study of consecutive patients undergoing rTARUP or IPOM repair for small or medium-sized primary ventral and incisional hernias. The primary outcome was postoperative need for transverse abdominis plane (TAP) block or epidural catheter, and secondary outcomes were 30-day complications, and length of stay. All patients underwent elective surgery and were followed for 30 days postoperatively.”

Results

“A total of 27 patients (rTARUP) and 32 (IPOM) were included. Patients in the two groups were comparable in terms of age, sex, comorbidities, smoking status, body mass index (BMI), and type of hernia. The median BMI was 32.4 kg/m2 and the fascial defect area was comparable (rTARUP median 16.8 cm2 vs. IPOM 11.7 cm2, P = 0.303). The duration of surgery was longer in the rTARUP group (median 117.2 min. vs. 84.4, P = 0.003), whereas the postoperative need for TAP block or epidural analgesics was less in the rTARUP group compared with IPOM (n = 14 vs. n = 1, P = 0.002). There were no severe complications or reoperations in the two groups. The length of stay was shorter in the rTARUP group (median 0 vs. 1 days, P < 0.001).”

Conclusions

“rTARUP was associated with reduced postoperative analgesic requirement and shorter length of stay compared with laparoscopic IPOM.”
P159 PARTIAL VS COMPLETE MESH REMOVAL AFTER CROHNIC MESH INFECTION: COMPARISON OF OUTCOMES
Authors: Anguiano-Diaz Gregorio , Tallon-Aguilar Luis , González Jose Tinoco , Arteaga Alejandro Sánchez , Gómez Daniel Díaz , Padillo Javier
Published: December 2021

Aim

“The purpose of this study is to compare the postoperative outcomes between partial mesh removal (PM) and complete mesh removal (CM) due to chronic mesh infection.”

Material and Methods

“Patients with mesh removal due to chronic mesh infection were included from February 2010 to May 2020. The patients were consequently assigned depending on the surgical technique to either partial or complete mesh removal. The demographic, operative and follow-up data of the two groups were analyzed and compared in terms of surgical site occurrence (SSO), surgical site infection (SSI), overall complications following Clavien-Dindo classification and relapse of mesh infection at 1 year follow-up.”

Results

“The study included 65 patients (44.61% males) intervened of mesh explantation. MThe patients were assigned to PM 56.92% (n = 37) and CM 43.08% (n = 28). 27% of the patient in the CM group needed a new mesh vs. 7.1 % of PM p = 0,039 (4.38 OR 1.02-24.1). There were no statistically significant differences with respect to length of hospital stay CM 5.46 (DS 6.1) vs 5.82 (DS 10.09) days, p = 0.409. SSO were 84.4%, p = 0.631, while SSI was 61.2%. There were no differences in terms of Clavien-Dindo (p = 0.617). The appearance of new chronic mesh infection after surgery were: CM 29.7% vs PM 39.3%, p = 0.420.”

Conclusions

“Postoperative morbidity after mesh explants is comparable between partial and total explants. Those cases in which a total explant is performed are more likely to require the placement of a new mesh, while in partial explant there is a higher percentage of recurrence of chronic infection.”
P160 ENDOSCOPIC ANTERIOR COMPONENT SEPARATION OR OPEN TRANSVERSUS ABDOMINUS RELEASE ARE NOT ASSOCIATED WITH INCREASED WOUND MORBIDITY FOLLOWING INCISIONAL HERNIA REPAIR
Authors: Gaspar Freia , Midtgaard Helle , Jorgensen Lars Nannestad , Jensen Kristian Kiim
Published: December 2021

Aim

Traditional anterior component separation during incisional hernia repair is associated with a high rate of postoperative wound morbidity. Because extensive subcutaneous dissection is avoided by endoscopic anterior component separation (eACS) or open transversus abdominis release (TAR), we hypothesized that these techniques did not increase the incidence of surgical site occurrence compared to incisional hernia repair without component separation.

Material and Methods

This was a retrospective cohort study of patients undergoing open, retro-rectus incisional hernia repair. Component separation during retro-rectus repair was performed using eACS or TAR. The primary outcome was 30-day incidence of postoperative surgical site occurrence. Secondary outcomes included length of stay, 30-day readmission, 30-day reoperation rate and 3-year recurrence rate.

Results

A total of 322 patients underwent retro-rectus repair, 168 (52%) of whom received either eACS or TAR. Addition of eACS or TAR was neither associated with surgical site occurrence, (odds ratio: 0.82, confidence interval: 0.40-1.68, P = 0.596) nor with hernia recurrence (hazard ratio 0.80, CI 0.27-2.40, P = 0.693). There was no significant difference between the groups regarding the frequencies of 30 day-readmission or 30-day reoperation.

Conclusions

The addition of eACS or TAR to a retro-rectus incisional hernia repair was not associated with increased wound morbidity or hernia recurrence.