bluuebunny/crossref_metadata_2025 huggingface dataset:
THE IMPACT OF BUSINESS INTELLIGENCE SYSTEMS ON PROJECT MANAGEMENT IN HIGHER EDUCATION: AN EMPIRICAL STUDY
Authors: NAIDU Vıkas Rao , AL BALUSHİ Huda , REALES Lilibeth , AL QASSABİ Suad , AL BALUSHİ Shaima , JESRANİ Karan , AL MEHDİ Zahra
Published: September 2023

The successful completion of projects depends on effective project management, particularly in the complex and dynamic environment of higher education institutions. Business intelligence (BI) tools can assist identify problems and hazards, facilitate decision-making, and offer insightful information on how projects are performing. The purpose of this study is to examine how BI systems are used for project management in higher education institutions and to suggest a successful project management strategy that incorporates BI systems. To determine the present state-of-the-art in project management and BI systems in higher education, the research technique includes a rigorous literature review of academic and industry sources. An analysis of a case study from a university that has used a BI system for project management is also included in the Study. The study's conclusions imply that BI systems can enhance project management at higher education institutions by supplying precise real-time data on project performance, risks, and problems. By facilitating better decision-making, early issue detection, and prompt problem resolution, the deployment of a BI system has improved project management, according to the case study analysis. The study suggests a successful project management strategy that incorporates BI systems in higher education institutions based on the findings. The following steps make up the suggested strategy: Project objectives and scope definition, project planning, BI system implementation, BI system performance monitoring, project issue and risk identification and mitigation, and continuous improvement are the steps in a project. The suggested framework can assist institutes of higher learning in managing projects successfully by making use of BI tools. The paper also outlines the critical success criteria for BI system deployment in project management in higher education institutions, such as top management support, transparent communication, user training, and efficient data governance.
Kryzys głodowy i jego skutki dla ludności niemieckiej w czasie I wojny światowej
Authors: Wojcieszak Andrzej
Published: September 2023

Od stuleci, a może tysiącleci podstawową przyczyną, obok nieurodzajów, pojawienia się głodu na świecie są konflikty zbrojne. Wielokrotnie, nawet w przypadku lokalnych potyczek, jednym ze sposobów uzyskania przewagi na polu walki zbrojnej było wywołanie stanu głodu pośród żołnierzy przeciwnej armii lub doprowadzenie do katastrofy humanitarnej wśród ludności cywilnej. Takie działanie było też często narzędziem nacisku politycznego, próbą uzyskania określonych korzyści, a czasem nawet karą za niesubordynację. Kryzys żywnościowy, na który narażona była ludność niemiecka w czasie I wojny światowej, jest zjawiskiem, które wpływało nie tylko na nastrój, życie codzienne i zdrowie ludności niemieckiej, ale ostatecznie także na przebieg wojny i rewolucji, na które ogromny wpływ miał rok 1918. W artykule autor podejmuje próbę nakreślenia trudności, na jakie narażona była ludność niemiecka z powodu kryzysu żywnościowego oraz jego wpływu na zdrowie i śmiertelność. Wiele miejsca poświęca przyczynom głodu ludności niemieckiej: po pierwsze skutkom alianckiej blokady morskiej, po drugie przyczynom spadku produkcji w rolnictwie, i po trzecie interwencyjnym działaniom państwa, które przyniosły efekt przeciwny do zamierzonego.
Penyandian Pesan Hybrid dengan Myszkowski Cipher dan Rivest, Shamir, Adleman (RSA)
Authors: Safitri Sukmawati Indah , Khudzaifah Muhammad , Jauhari Mohammad Nafie
Published: September 2023

Classical and modern cryptography have advantages and disadvantages in securing text messages. Hybrid algorithms can combine two algorithms to produce a higher level of message security when compared to one type of algorithm. This study discusses a hybrid algorithm that combines Myszkowski Cipher and RSA. The purpose of this research is to obtain ciphertext that is more difficult to crack by cryptanalysis. The hybrid algorithm process is done by encrypting text messages with the Myszkowski Cipher algorithm then the key used in the Myszkowski Cipher algorithm are encrypted using the RSA algorithm with the equation . Then the ciphertext and cipherkey will be obtained in numerical. The decryption process is done by decrypting the cipherkey by specifying the value of  as the private key so that  using the equation . Next is by trying the value of  until a  value is obtained, after which decryption to the equation , then the process of decrypting ciphertext using Myszkowski Cipher can be done. The benefit of this research is can improve the security of messages because it applies the advantages of each algorithm in its encoding.
Manual Fundamentos y técnicas básicas en microbiología
Authors: Lizarazo Forero Luz Marina
Published: September 2023

La versatilidad microbiana hace que los microorganismos jueguen un rol importante en la conservación del medio ambiente, la salud y la industria. Ellos constituyen también excelentes sistemas modelo para el estudio de la genética molecular y la fisiología celular. De igual forma, el conocimiento de nuevas técnicas, como la tecnología del ADN recombinante, ha revolucionado tanto la investigación como la aplicación práctica en todas las áreas de la microbiología.Dado el importante valor que representa esta disciplina, he desarrollado este Manual fundamentos y técnicas básicas en microbiología, con el cual pretendo poner a disposición de los usuarios  los  conocimientos  esenciales  y  actualizados  de  los procedimientos básicos utilizados en microbiología; asimismo este libro constituye una herramienta para el manejo de técnicas que permitan despertar en el estudiante el deseo de conocer e investigar más allá de lo adquirido durante el curso. El texto está dirigido a estudiantes de ciencias básicas, ciencias agrícolas, ingenierías, e incluye temas tales como: medidas de bioseguridad, preparación de medios de cultivo, técnicas de esterilización,   siembra   e   identificación   de   microorganismos, fisiología, control del crecimiento microbiano y antibiosis .
Puntos de vista.
Authors: Páez Guzmán Esaú Ricardo , Montero González Martha Soledad , Cabrera Díaz Andrés Mauricio , Cantillo Barrera Alexandra Patricia , García Salazar Edwin , Rubiano Suza Christian Alfredo , Molina Bravo José Arturo , Bernal Mancilla Johanna Andrea
Published: September 2023

El libro "Puntos de vista entre Filosofía y escritura" es el resultado del proyecto de investigación sobre la formación de nuevos investigadores integrantes del Grupo Filosofía, educación y pedagogía. Expone a los investigadores en formación del grupo a un ejercicio problemático de producción, demostración, sustentación y escritura, bien sea como punto de partida o de llegada de investigaciones iniciales y avanzadas o de resultados, en el sentido de abrir posibilidades de experimentación y experiencia en un espacio que conecta y entrecruza autores, escritores, artistas, profesores para poner en movimiento la fuerza de pensar la filosofía, el texto, la literatura, el lenguaje, la enseñanza de la escritura en el mundo escolar de hoy en día, el oficio del maestro en su pragmática universitaria, la conversación sobre como se escribe, el papel del lector y la existencia del libro, la manera como los niños se conectan con la lectura y la escritura, la simple lectura de los libros de literatura y poesía por la experiencia estética y artística de vivir y escuchar voces que nos hablan al oído sobre lo conocido y lo desconocido de los mundos y de la existencia: abandonados, expuestos, actuando, decidiendo, desafiando la propia vida. Puesto que, en el campo de la educación y la pedagogía se pasa por la exigencia de escribir. Esto pone en cuestión en el campo de la educación lo que, al parecer, se aviene bien con la instrumentalización del pensamiento, con los derroteros, los observatorios y observadores según la especulación del uso del conocimiento, únicamente a partir de la ciencia y de la técnica reconocido como lo válido y oficial en el engranaje institucional del Estado colombiano y en la vida académica internacional, como sí estos elementos fueran las únicas narrativas que le dan forma y pertinencia a la educación, al aprender, al saber y al deseo de saber. Soledad Montero G.
Structural and cultural constraints on adopting tsabo-based agroforestry as an alternative to tavy around Betampona Reserve, Madagascar
Authors: Rakotondratandra Daorotiana
Published: September 2023

Madagascar is known worldwide for its exceptional biodiversity. However, slash-and-burn cultivation (tavy) accounts for 80 to 95% of deforestation in this country. Despite decades of efforts to promote tsabo-based agroforestry as a sustainable alternative, the tavy agricultural technique has converted 50% of the original surface area of the Betampona Strict Nature Reserve (about 1,200 ha) in eastern Madagascar into cropland or secondary forests. Applying an interdisciplinary approach with an integrated theoretical framework, this study attempts to capture the constraints on the adoption and extension of tsabo-based agroforestry as an alternative to tavy on smallholder farms in the buffer zone around this protected area. Although tsabo-based agroforestry provides up to 67.6% of their cash income from farming, 20% to 43% of the households sampled no longer wish to establish new tsabo-based agroforestry plots or to extend their existing ones; 40.6% and 62.7% of households have continued the practices of monoculture and tavy, respectively. This article concludes that the factors impeding the establishment or extension of tsabo-based cultivation in the study area include structural as well as cultural dimensions. On the one hand, worsening geographic isolation, rural insecurity and poverty, together with the complete breakdown of marketing channels for their agricultural products, are discouraging farmers from extending their tsabo plots. On the other hand, even though rice is part of the Malagasy identity and governs all aspects of village culture, projects for tsabo extension have failed to integrate rice within the agroforestry systems they propose to farmers. It is recommended that policymakers and development programs take these structural and cultural factors into account in order to design agroforestry systems that correspond to the producers’ needs.
A Santonian record of the nautilid cephalopod Angulithes westphalicus (Schlüter, 1872) from the subsurface of the Campine, north-east Belgium, with comments on regional lithostratigraphic problems
Authors: GOOLAERTS STIJN , MOTTEQUIN BERNARD
Published: September 2023

Newly recognised material of the Late Cretaceous nautilid Angulithes westphalicus (Schlüter, 1872) is described from the subsurface of the eastern part of the Campine in north-east Belgium. This constitutes the first formal documentation of this genus and species from the Cretaceous of Belgium, having been identified amongst a large suite of fossils collected from the Voort Shafts I & II of the Zolder colliery during the first half of the twentieth century. The specimens originate from an interval of marine calcareous sand with a marly glauconiferous base, dated as late middle Santonian (Gonioteuthis westfalicagranulata belemnite Zone) and for which a deepening of the depositional environment is documented. Lithostratigraphically, the specimens occur within the Vaals Formation, within the upper part of the Asdonk Member or alternatively within the lower part of the Sonnisheide Member. The early Campanian age of the Asdonk Member suggested previously is refuted, the age of the Sonnisheide Member needs further study. The position of the siphuncle in A. westphalicus is illustrated for the first time; it is positioned closer to the venter than the dorsum, which confirms the close evolutionary relationship with Angulithes galea (Fritsch in Fritsch & Schlönbach, 1872), which ranges from the upper Turonian to middle Coniacian in central Europe.
Proof of Two-dimensional Jacobian Conjecture
Authors: Su Yucai
Published: September 2023

Using the local bijectivity of Keller maps, we give a proof of two-dimensional Jacobian conjecture.
Clown Doctors in the Lento Tempo of Pediatric Oncology: A Dialogue between Psychology and Art on the Meaning of an Encounter When Life Meaning Is Disrupted
Authors: Peceguina Inês , Alcântara Iêda , González António José
Published: September 2023

Using a humanistic and qualitative approach, the present study aims to (1) bring light on 1 the impact of clown doctors’ artistic work in pediatric oncology and, as a consequence, (2) contribute 2 to the refinement and improvement of the clown doctors’ intervention quality, in the context of 3 pediatric oncology, bringing hypotheses of reflection hardly met with quantitative approaches. We 4 are interested in the subjective experience of the artists, and the perceived subjective experience of 5 the child as reported by the clown doctors. The present analysis was developed from the clown 6 doctors’ final reports, their narrative, after visiting the pediatric oncology ward in a Portuguese public 7 hospital, for a continuous period of six months. The visits were performed by a clown doctor dyad, 8 and the audience was a young adolescent girl, with cancer, and her constant mother.9
Target Localization and Grasping of Parallel Robots with Multi-Vision Based on Improved RANSAC Algorithm
Authors: Gao Ruizhen , Li Yang , Liu Zhiqiang , Zhang Shuai
Published: September 2023

Some traditional robots are based on offline programming reciprocal motion, and with the continuous upgrading of vision technology, more and more tasks are replaced by machine vision. For the current problem of insufficient accuracy of robot target localization based on binocular vision, and an improved random sampling consistency algorithm is proposed to complete parallel robot target localization and grasping under the guidance For the current problem of insufficient accuracy of robot target localization based on binocular vision, an improved random sampling consistency algorithm is proposed to complete parallel robot target localization and grasping under the guidance of multi Firstly, the RANSAC algorithm is improved based on the SURF algorithm; then the parallax gradient method is applied to iterate the matched point pairs several times to further optimize the data; then the 3D reconstruction is completed by the program technique; finally the obtained data is finally the obtained data is input into the robot arm and the camera internal and external parameters are obtained by the calibration method so that the robot can accurately locate and The experiments show that the improved algorithm has advantages in recognition accuracy and grasping success rate under multi- The experiments show that the improved algorithm has advantages in recognition accuracy and grasping success rate under multi-vision system.
PENGARUH KETIDAKSESUAIAN ADMINISTRASI KEPABEANAN PADA FASILITAS KAWASAN BERIKAT TERHADAP KEGIATAN EKSPOR STUDI KASUS PT 888 WEST SEHATI
Authors: Rajendra Igo Maulanatazi  , Rohmawan Adi Pratama  , Islamiyah
Published: September 2023

Perdagangan internasional menyebabkan seluruh negara di dunia semakin mudah terintegrasi antara satu negara dengan negara lainnya. Pemerintah Indonesia membuat kebijakan dengan memberikan fasilitas kawasan berikat yang merupakan fasilitas kepabeanan yang diberikan kepada para pelaku usaha yang produk utamanya berorientasi ekspor. Analisis ini bertujuan untuk mengetahui ketidaksesuaian penerapan administrasi kepabeanan di kawasan berikat terhadap kegiatan ekspor pada PT 888 West Sehati. Selain itu, penelitian juga akan membahas tentang pengaruh yang terjadi atas kasus ketidaksesuaian administrasi kepabeanan terhadap kegiatan ekspor. Metode yang digunakan dalam penelitian ini menggunakan analisis kualitatif di mana penelitian dilakukan dengan membaca dan memahami buku-buku referensi, jurnal, surat Keputusan, peraturan, dan undang-undang yang berdasarkan kepabeanan fasilitas kawasan berikat.  Metode lain yang digunakan penulis adalah deskriptif yaitu mendeskripsikan fakta yang ditemukan selama penelitian untuk merumuskan strategi yang tepat bagi PT 888 West Sehati. Hasil dari penelitian ini yaitu pengaruh ketidaksesuaian administrasi kepabeanan fasilitas kawasan berikat terhadap kegiatan ekspor adalah perusahaan tidak dapat melakukan kegiatan ekspor dan tertahan oleh petugas bea cukai yang berakibat kerugian pada perusahaan. Saran yang diberikan untuk mengatasi ketidaksesuaian administrasi kepabeanan yaitu menerapkan proses tata laksana kepabeanan sesuai peraturan yang telah ditetapkan oleh Direktorat Jenderal Bea dan Cukai.
ANALISIS BISNIS JASA ANGKUTAN BARANG RETAIL PADA PT KERETA API INDONESIA (PERSERO) DAOP 5 PURWOKERTO
Authors: Laila Fitriana  , Dama Yuninata
Published: September 2023

Penelitian ini dilakukan untuk mengetahui layanan bisnis kereta api yang efektif dalam pengangkutan barang antara proses business to business dan business to customer sehingga muatan atau volume yang diangkut mencapai target atau program. Metode penelitian yang digunakan adalah metode deskriptif kualitatif. Metode ini digunakan untuk memperoleh gambaran tentang layanan bisnis pada angkutan barang perkeretaapian. Pengumpulan data dilakukan dengan wawancara, observasi, dan telaah dokumen (studi pustaka). Analisis data dilakukan melalui uji anova yaitu dengan membandingkan banyaknya muatan yang diangkut dengan dua layanan bisnis yang berbeda dalam pengiriman barang kereta api.
PELANGGARAN ETIKA BISNIS MENJUAL PRODUK BERSEGMENTASI KHUSUS DI MINIMARKET
Authors: Niko Dwi Haryanto  , Andini Mareta  , Ervina Islamiati
Published: September 2023

Etika bisnis didefinisikan sebagai kebiasaan baik dan pentingnya kebutuhan etika dalam bisnis tidak bisa diabaikan, prinsip etika bisnis mencakup pelaku bisnis mulai dari proses perencanaan sampai dengan pengawasan. Pelanggaran etika bisnis dalam menjual produk bersegmentasi khusus di minimarket tentu dapat membentuk citra yang buruk. Studi ini menganalisis penyebab adanya pelanggaran etika bisnis dalam menjual produk bersegmentasi khusus di minimarket. Penelitian ini menggunakan pendekatan deskriptif kualitatif dengan literatur dan observasi sebagai sumber referensi. Hasil pada penelitian ini menunjukkan bahwa pelanggaran bisnis pada konteks ini harus diperbaiki dengan adanya prosedur, peran pemerintah atau badan yang berwenang dan peran masyarakat dalam melaporkan adanya pelanggaran etika bisnis tersebut untuk meminimalisir pelanggaran etika yang harus terjadi. Kesimpulan pada penelitian ini adalah tidak hanya sistem yang perlu diperbaiki, tetapi budaya dari manajemen minimarket itu sendiri yang harus memulai terlebih dahulu.
First Record of the Small-billed Elaenia (Elaenia parvirostris) for Western North America
Authors: Clements Nolan M. , Tietz James R.
Published: September 2023

The Small-billed Elaenia (Elaenia parvirostris) is a small flycatcher common on the Atlantic slope of South America. The species breeds below 1000 m elevation in open woodlands, gardens, and forest edges from southern Brazil to northeastern Argentina and migrates north to spend the nonbreeding season in the northern Amazon Basin. Since 2012, it has been documented as a vagrant to North America, with four records from the United States and Canada to date. On the basis of one photographed on Southeast Farallon Island on 4 September 2022, we record a fifth Small-billed Elaenia in North America, a first for California and the Pacific coast. Its novelty notwithstanding, it represents an outlier of an established pattern of vagrancy of tyrant flycatchers from South America reaching North America via overshooting or reverse migration.
An Eccentric Preformative Molt with Incomplete Replacement of Primary Coverts in a Dark-eyed Junco
Authors: Tattoni D. Julian
Published: September 2023

Though the Dark-eyed Junco has not been reported to replace any juvenile primaries during its preformative molt, one first-cycle bird captured at Stanford, California, in November 2022 had replaced all its remiges but the three innermost primaries. Thus it followed the eccentric pattern more frequently seen in other sparrows, wrens, and some tyrant flycatchers. This novel pattern may be an adaptation to the urban habitats that the junco has recently colonized.
3D printed resin composite posterior fixed dental prosthesis: A prospective clinical trial up to 1 year
Authors: Ordueri Tugce , Hobbi Paniz , Öztürk-Bozkurt Funda , Toz-Akalın Tugba , Ateş Muzaffer , Özcan Mutlu
Published: September 2023

Objectives: This clinical trial evaluated the clinical behaviour of 3D printed posterior resin composite fixed dental prosthesis restorations.
Materials and Methods: Between 10.10.2020 - 05.08.2022, forty-nine patients aged between 19 and 60 years old (33 females, 16 males) received 68 3D printed resin composite 3-unit posterior fixed dental prosthesis (ELS Even Stronger, Saremco, Switzerland). FDPs were followed up 2 weeks after placement (baseline) by two independent calibrated observers using modified FDI criteria.
Results: Fifty-two FDPs completed their 6 months, 23 and 1of the resin composite FDPs their 1 and 2- year follow-up, respectively. Mean observation period was 8.63 months. Failure types were categorized as mechanical and biological ones. At the end of these observation periods, 8 FDPs showed cohesive fractures. Three abutment teeth needed endodontic treatment, 2 periodontal problems. One FDP was rebonded due to retention failure. After one year, 2 FDPs showed surface lusture loss (Score 4), 2 colour mismatch (Score 4), 2-surface staining (Score 4). Based on mechanical failures, survival rate was 86.7% while including the biological complications, this corresponded to 71.6% (Kaplan-Meier).
Conclusion: Long-term follow-up of the present cohort will indicate whether the tested material may serve as long-term provisional or even as permanent material for three-unit FDPs.
Clinical Relevance: Resin composite with improved properties could be preferred for permanent prosthetic applications rather than temporary approaches with current 3D technology and CAD / CAM applications.
Clinical Trial Registration Number: NCT04600297
Self-assembly and peripheral guest-binding of a sandwich-like complex [ZnII3L2]6+
Authors: Zhao Juan-Tong , Wu Wen-Yuan , Lou Yi-Chun , Cai Wei-Jie , Wan Rong
Published: September 2023

A novel complex [ZnII3L2]6+ was designed and then synthesized by using subcomponent self-assembly of zinc trifluoromethanesulfonate (Zn(OTf)2), 2,4,6-tri(3-aminophenoxy) triazine, and 2-formylpyridine in CH3CN solution. The target compound's structure as a sandwich-like helicate was verified by 1H NMR and ESI-MS measurements, and the host-guest interactions between the complex and a number of guest molecules were investigated. It was discovered that the effect of the host-guest interaction significantly changed when the aldehyde component was replaced by 3-methylpyridine-2-carboxaldehyde, which suggested that the guest-binding sites may occur around the peripheral apertures of the complex. Also the binding behavior of the tetraphenylborate guests was further investigated, which quantified the host-guest stoichiometry in the ratio of 1:2.
Polycystic intrahepatic infection caused by Enterococcus casseliflavus: A case report and literature review
Authors: Xu Senyin , Huang Bin , Cao Youjun , Zhong Zhongyong , Yin Jiazhen
Published: September 2023

Background: Enterococcus casseliflavus is a rare pathogenic bacterium that is characterized by vancomycin resistance and can lead to multiple infections in the human body. This report describes a rare case of polycystic intrahepatic infection with E. casseliflavus which necessitated antibiotic treatment and surgical intervention involving cystic drainage.
Case Presentation: A 59-year-old woman, a long-term hemodialysis patient, was hospitalized due to a 5-day history of fever, abdominal pain, and diarrhea, which were possibly caused by the ingestion of contaminated food. Her blood culture yielded a positive result for E. casseliflavus, and she was initially treated with piperacillin/tazobactam and linezolid. Later, the antibiotic regimen was adjusted to include meropenem and linezolid. Despite treatment, her body temperature remained elevated. However, subsequent blood cultures were negative for E.casseliflavus.Conventional CT scans and ultrasound examinations did not identify the source of infection. However, a PET-CT examination indicated an intrahepatic cyst infection. Following MRI and ultrasound localization, percutaneous intrahepatic puncture and drainage were performed on the 20th day. Fluoroquinolones were administered for 48 days. On the 32nd day, MRI revealed a separation within the infected cyst, leading to a repeat percutaneous drainage at a different site. Subsequently, the patient's temperature returned to normal. The infection was considered resolved, and she was discharged on the 62nd day. Follow-up results have been favorable thus far.
Conclusions: Based on the findings from this case, it is recommended to promptly conduct PET-CT examination to exclude the possibility of intracystic infection in cases of polycystic liver infection that are challenging to control. Furthermore, timely consideration should be given to puncture drainage in difficult cases.
On the convergence of uncertain set processes
Authors: Ghaffari-Hadigheh ‎Alireza , Vajargah Behrouz Fathi
Published: September 2023

Recently, the set-valued uncertain process has been introduced that is a sequence of uncertain sets evolving as time goes ahead. The concepts of convergence of set-valued uncertain processes were also defined from different points of view. Here, we elaborate on these definitions and investigate the relationship between them. We prove that some convergence concepts imply others while the inverse is not generally valid. We also propose a novel measure of convergence and verify its relationship with the others. Each finding is described with simple illustrative examples.
Quantitative Evaluation of a Quasi-Fermi Level of the Hole at a Photoanode/Electrolyte Interface
Authors: Takanabe Kazuhiro , Shioiri Yuu , Obata Keisuke , Kawase Yudai , Higashi Tomohiro , Katayama Masao
Published: September 2023

Valence band maximum (VBM) position does not necessarily indicate the driving force for oxidation reactions on photoanodes because it simply reflects an electrostatic potential. Rather, the quasi-Fermi level of the hole at the surface of photoanodes must be considered. Here, we report a protocol to quantitatively evaluate the quasi-Fermi level of the holes at the surface of photoanodes using redox species with various redox potentials. The quasi-Fermi level of these holes is estimated by correlating the photocurrent derived from redox species oxidation to the separately measured electrode potential on a stable model glassy carbon electrode. Using this protocol, the quasi-Fermi levels of holes on the surface of CdS and CdSe model photoanodes were found to be limited to merely 0.5 V vs. standard hydrogen electrode; this is fairly negative from the VBM position. The estimation of a quasi-Fermi level of the hole and the partial photocurrent density of the CdS and CdSe photocorrosion further enabled predictions of the photocorrosion kinetics. The predicted kinetics of the photocorrosion showed that the quasi-Fermi levels of hole at the surface of these photoanodes are pinned by the facile kinetics of the photocorrosion. This methodology offers a quantitative understanding of the oxidation capability of photoanodes.
Discrepant phenotyping of monocytes based on CX3CR1 using fluorescent reporters and antibodies
Authors: Sommer Katrin , Garibagaoglu Hilal , Wiendl Maximilian , Müller Tanja M. , Atreya Imke , Krönke Gerhard , Neurath Markus F. , Zundler Sebastian
Published: September 2023

Monocytes as well as downstream macrophages and dendritic cells are essential players of the immune system fulfilling key roles in homeostasis as well as in inflammatory conditions. Conventionally, driven by studies in reporter models, mouse monocytes are divided into a classical and a non-classical subset based on their inversely correlating surface expression of Ly6C and CX3CR1. Here, we analysed the expression of CX3CR1 by flow cytometry using several validated fluorochrome-coupled CX3CR1 antibodies and compared them with the reporter gene signal of a Cx3cr1GFP reporter mouse strain as well as of tamoxifen-inducible Cx3cr1 reporter mice. Although we were able to validate the specificity of several fluorochrome-coupled CX3CR1 flow cytometry antibodies, mouse Ly6Chigh classical and Ly6Clow non-classical monocytes showed no differences in CX3CR1 expression levels in peripheral blood and spleen, when stained with these antibodies. To the contrary, in reporter mice, we were able to reproduce the inverse correlation of CX3CR1 reporter gene signal and Ly6C surface expression. As determined by qPCR, the Cx3cr1 mRNA expression correlated with the GFP-reporter gene expression as quantified by flow cytometry. In conclusion, our data suggest that there is differential transcription, but not surface expression of CX3CR1 between classical and non-classical monocytes, which limits the suitability of CX3CR1 for phenotyping monocyte subsets by antibody staining.
A Rare Gene Mutation Disorder With Fever To Be Detected
Authors: Li Shanshan , Liu Na , Yu Jianmin , Tou Fangfang , Tong Bo
Published: September 2023

ROSAH syndrome(1) is an autosomal dominant disorder caused by mutations in ALPK1. Its clinical manifestations are mainly retinal dystrophy, optic nerve edema, splenomegaly, anhidrosis and migraine. In this article, we aim to provide detailed information that will help further our understanding of the different symptoms and manifestations of ROSAH syndrome. It also emphasizes the importance of considering this disease in similar cases of fever of unknown etiology, such as recurrent periodic fever with splenomegaly and ocular disease.
Identification and Analysis of Hub Transcriptional Factors in vitro hepatocyte model of Acetaminophen- Induced Liver Injury
Authors: Zhao Qiang , Bai Jinjia , Zhao Ninghui , Yao Jia
Published: September 2023

Background
Acetaminophen-induced liver injury (AILI) is one of the most common causes of acute liver failure. It becomes more recognized that Transcription factors are involved in morphology alterations of hepatocyte in AILI patients. The integrated analysis of the Transcription factors role in the pathogenesis in AILI remains unknown.
Material and Methods
Based on bioinformatics, GEO databases and TF databases,we identify and analyze the hub TFs in vitro hepatocyte model of AILI and further evaluate the value of these TFs in the early diagnosis of AILI.
Results
A total of 97 AILI-related differentially expressed transcriptional factors (DETFs) were obtained, which were mainly enriched in the transcriptional activity, rhythmic process, cell fate commitment, liver development, and hepaticobiliary system development. Ten hub TFs (MYC, TP53, CEBPB, FOXM1, E2F1, EGR2, FOSL1, JUND, E2F7 and E2F8) were obtained from the PPI networks. In the early stage of AILI, qRT-PCR showed that the expressions level of MYC, TP53, CEBPB, E2F1, JUND, and E2F7 significantly changed compared with the control group (all P < 0.05), and these hub TFs had high sensitivity and specificity(all AUC ≥ 0.9); in contrast, the expressions level of FOXM1, EGR2, FOSL1 and E2F8 were not significantly different from those in the control group (all P＞0.05), and these hub TFs had certain sensitivity and specificity(all 0.67 ≤ AUC ≤ 0.79).
Rapid detection of Avian Influenza Virus based on CRISPR-Cas12a
Authors: Zhou Xu , Wang Siwen , Ma Yue , Li Yanbing , Deng Guohua , Shi Jianzhong , Wang Xiurong
Published: September 2023

Background: Avian influenza(AI) refers to the disease caused by infection with avian influenza viruses(AIV). These viruses naturally spread among wild aquatic birds worldwide and can infect domestic poultry, other birds, and animal species. At present, real-time Reverse Transcription-Polymerase Chain Reaction (rRT-PCR) is mainly used to detect the presence of pathogens, which has good sensitivity and specificity. However, the diagnosis requires sophisticated instruments under laboratory conditions, which significantly limits the point-of-care testing (POCT). A rapid, reliable, non-lab equipment reliant, sensitive, and specific diagnostic test is urgently needed in the field of clinical rapid detection and diagnosis.
Methods: In this study, Cas12a protein was purified using affinity chromatography with a Ni-Agarose resin and observed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The specific CRISPR-RNA (crRNA) and primers targeting the M and NP genes of AIV were designed and screened out. By combining reverse transcription recombinase polymerase amplification(RT-RPA) with the Cas12a/crRNA trans-cleavage system, the detection system through fluorescence readouts under blue light or using lateral flow strips was established. The sensitivity assays were carried out using a 10-fold dilution series of the plasmids and RNA of M and NP genes as the templates. The specificity of this method was determined by using H1~H16 subtypes AIVs and other avian pathogens such as Newcastle Disease Virus (NDV), Infectious Bursal Disease Virus (IBDV) and Infectious Bronchitis Virus (IBV).
Results:The results showed that the method was able to detect AIV and the detection limit can reach 6.7 copies/μL and 12 copies/μL for the M and NP gene, respectively. In addition, this assay showed no cross-reactivity with other avian-derived RNA viruses such as NDV, IBDV, and IBV. Moreover, the detection system presented 97.5% consistency and agreeability by comparing with the rRT-PCR and virus isolation in detecting samples from poultry. This portable and accurate method holds great application potential for detecting AIV in the field.
Conclusion: In summary, a RT-RPA/CRISPR method was developed for the rapid and sensitive detection of AIV. The new system presents a good potential to be an accurate, user-friendly, inexpensive platform for point-of-care testing applications.
Simultaneous analysis of the of levamisole with triclabendazole in pharmaceuticals through developing TLC and HPLC-PDA chromatographic techniques and their greenness assessment using GAPI and AGREE methods
Authors: Attia Khalid A.M. , El-Desouky Ebrahim A. , Abdelfatah Amr M. , Abdelshafi Nahla A.
Published: September 2023

Two simple and rapid chromatographic methods were developed and validated for the analysis of levamisole and triclabendazole simultaneously in pure and pharmaceutical products. The first method is thin-layer chromatography (TLC) with densitometry, and the second method is high-performance liquid chromatography with PDA detection (HPLC-PDA). A Hypersil BDS C18 column with dimensions of 4.6 x 150 mm and a particle size of 5 µm was used in the HPLC-PDA method. An isocratic condition was used to carry out the separation, and the mobile phase was made up of acetonitrile and a 0.03 M potassium dihydrogen phosphate buffer in double-distilled water. The ratio of the mobile phase preparation was 70:30 (v/v), and the flow rate was 1 mL/min. A wavelength of 215 nm was employed for analyte detection. Precoated silica gel 60 F254 aluminum plates were used for the TLC method's separation. Mobile phase was made of ethyl acetate, hexane, methanol, and ammonia (69:15:15:1) for the separation. The detection wavelength selected was 215 nm. According to the International Council for Harmonization (ICH) guidelines, the proposed methods were validated, and it was found that the two chromatographic methods are accurate, precise, and linear for both compounds in the range of 3.75–37.5 and 6–60 mg/L for the HPLC method for levamisole and triclabendazole, respectively and in the range of 2–14 µg/spot for the TLC method. The developed methods greenness profile was assessed using AGREE and ComplexGAPI tools.
Reduced suprasellar cistern cerebrospinal fluid motion in patients with Parkinson’s disease revealed by magnetic resonance imaging with dynamic cycling of diffusion weightings
Authors: Mays Gabriela Pierobon , Hett Kilian , Eisma Jarrod , McKnight Colin D. , Elenberger Jason , Song Alexander K. , Considine Ciaran , Han Caleb , Stark Adam , Claassen Daniel O. , Donahue Manus J.
Published: September 2023

BACKGROUND: Parkinson’s disease is characterized by dopamine-responsive symptoms as well as aggregation and accumulation of a-synuclein protofibrils. New diagnostic methods assess a-synuclein aggregation characteristics from cerebrospinal fluid and recent pathophysiologic mechanisms suggest that cerebrospinal fluid circulation disruptions may precipitate a-synuclein retention. Here, we test the hypothesis that cerebrospinal fluid motion at the level of the suprasellar cistern is reduced in Parkinson’s disease relative to healthy participants and this reduction relates to choroid plexus perfusion.
METHODS: Diffusion weighted imaging (spatial resolution=1.8x1.8x4 mm) magnetic resonance imaging with cycling of diffusion weightings (b-values=0, 50, 100, 200, 300, 700, and 1000 s/mm2) over the approximate kinetic range of suprasellar cistern neurofluid motion was applied at 3-Tesla in Parkinson’s disease (n=27; age=66±6.7 years) and healthy (n=32; age=68±8.9 years) participants. Wilcoxon rank-sum tests were applied to test the primary hypothesis that the decay rate of cerebrospinal fluid signal as a function of b-value, which reflects increasing fluid motion, is reduced in persons with versus without Parkinson’s disease and inversely relates to choroid plexus activity assessed from perfusion-weighted magnetic resonance imaging (Spearman rank-order correlation; significance-criteria: p<0.05).
RESULTS: Consistent with the primary hypothesis, decay rates were higher in healthy (D=0.00328±0.00123mm2/s) relative to Parkinson’s disease (D=0.00256±0.0094mm2/s) participants (p=0.016). This finding was preserved after controlling for age and sex. An inverse correlation between choroid plexus perfusion and decay rate (p=0.011) was observed in Parkinson’s disease participants.
CONCLUSIONS: Cerebrospinal fluid motion at the level of the suprasellar cistern is often reduced in adults with versus without Parkinson’s disease and this reduction correlates on average with choroid plexus perfusion.
Development and validation of a nomogram for predicting in-hospital mortality in patients with nonhip femoral fractures
Authors: XING ZHIbin , WU YUxuan , XU YIwen , FU XIAOchen , SHEN PENGfei , WANG JING
Published: September 2023

Background The incidence of nonhip femoral fractures is gradually increasing, but no studies have explored the risk factors for in-hospital death in patients with nonhip femoral fractures in the ICU or developed mortality prediction models. Therefore, we chose to study this specific patient group, hoping to help clinicians improve the prognosis of patients.Methods The ROC curve was drawn, and the AUC, NRI and IDI were calculated to evaluate the discrimination of the model. The consistency between the actual probability and the predicted probability was assessed by the calibration curve and HL test. DCA was performed, and the nomogram was compared with the scoring system commonly used in clinical practice to evaluate the clinical net benefit.Results The least absolute shrinkage and selection operator (LASSO) regression analysis showed that heart rate, temperature, red blood cell distribution width, blood urea nitrogen, GCS, SA PSII, Charlson comorbidity index and cerebrovascular disease were independent risk factors for in-hospital death in patients with nonhip femoral fractures. The areas under the curve (AUC), integrated discrimination improvement (IDI) and net reclassification index (NRI) of our model in the training set and validation set were better than those of the GCS and SAPSII scoring systems. The calibration curve and HL test results showed that our model prediction results were in good agreement with the actual results (P = 0.833 for the HL test of the training set and P = 0.767 for the HL test of the validation set). Decision curve analysis (DCA) showed that our model had a better clinical net benefit than the GCS and SAPSII scoring systems..Conclusion In this study, the independent risk factors for in-hospital death in patients with nonhip femoral fractures were determined, and a prediction model was constructed. The results of this study may help to improve the clinical prognosis of patients with nonhip femoral fractures.
Network Pharmacology and Molecular Docking-based Investigation of Monocyte Locomotion Inhibitory Factor Attenuates Traumatic Brain Injury by Regulating Aquaporin 4 Expression
Authors: Li Xinyu , Ma Yulin , Lv Mengting , Gao Yuan , Zhang Yuefan , Li Tiejun
Published: September 2023

Traumatic brain injury (TBI) is a significant cause of disability and mortality worldwide, and effective treatment options are currently limited. Monocyte locomotion inhibitor factor (MLIF), a small molecular pentapeptide, has demonstrated a protective effect against cerebral ischemia. This study aimed to investigate the protective effects of MLIF on TBI and explore its underlying mechanism of action. In animal experiments, we observed that administration of MLIF after TBI reduced brain water content and improved brain edema, suggesting a certain degree of protection against TBI. By utilizing of network pharmacology methodologies, we employed target screening techniques to identify the potential targets of MLIF in the context of TBI. As a result, we successfully enriched 10 signaling pathways that are closely associated with TBI. Furthermore, using molecular docking techniques, we identified AQP4 as one of the top 10 central genes discovered in this study. Eventually, our study demonstrated that MLIF exhibits anti-apoptotic properties and suppresses the expression of AQP4 protein, thus playing a protective role in traumatic brain injury. This conclusion was supported by TUNEL staining and the evaluation of Bcl-2, Bax, and AQP4 protein levels. These discoveries enhance our comprehension of the mechanisms by which MLIF exerts its protective effects and highlight its potential as a promising therapeutic intervention for TBI treatment.
Rice blast-induced jasmonic acid signal transduction affects rice resistance against brown planthopper by regulating the flavonoid biosynthesis pathway
Authors: Chen Su , Shen Yanjie , Tao Zhihuan , Chen Zixu , Yang Rui , Yan Siyuan , Sun Bo , Yang Xiaofang
Published: September 2023

The complex interactions among plants, herbivorous insects, and microbes form an iron triangle in nature, and even a minor alteration in any of these components can trigger a chain of interconnected reactions. In our study on the interaction between rice plants, brown planthopper (BPH), and rice blast fungus, we discovered that the resistance of rice treated with rice blast to BPH was significantly increased. Transcriptomic and mass spectrometric results showed that rice blast fungus treatment induced the contents of eriodictyol, naringenin, quercetin, as well as the expression of most genes in the flavonoid biosynthesis pathway except OsF3'H. Correspondingly, OsF3′H knockout lines enhanced rice resistance to BPH. OsMYC2 is a core transcription factor in the jasmonic acid (JA) signaling pathway and rice blast fungus induced the expression of OsMYC2, OsMYC2 knockout lines reduced rice resistance to BPH. Furthermore, a series of experiments demonstrated that OsMYC2 has a transcriptional repressive effect on OsF3'H. Overall, we revealed the phenomena and reasons behind the increased resistance to BPH after treating rice with rice blast fungus in terms of histological data, experimental phenotypes, and gene expression, respectively. This will deepen our understanding of the interactions among rice, rice fungus and BPH, and provide theoretical support for ecological management.
Developments of a centimeter-level precise muometric wireless navigation system (MuWNS-V) and its first demonstration using directional information from tracking detectors
Authors: Varga Dezső , Tanaka Hiroyuki K. M.
Published: September 2023

Various positioning techniques such as Wi-Fi positioning system have been proposed to use in situations where satellite navigation is unavailable. One such system, the muometric positioning system (muPS), was invented for navigation which operates in locations where even radio waves cannot reach such as underwater or underground. muPS takes advantage of a key feature of its probe, cosmic-ray muons, which travel straightforwardly at almost a speed of light in vacuum regardless of the matter they traverse. Similar to other positioning techniques, muPS is a technique to determine the position of a client's muPS receiver within the coordinate defined by reference detectors. This can be achieved either by using time-of-flight (ToF) or angle of arrival (AoA) measurements. The latter configuration (AoA), called the Vector-muPS has recently been invented and the present paper describes the developments of the first prototype of a vector muometric wireless navigation system (MuWNS-V) with this new vector-muPS concept and its demonstration. With MuWNS-V, the reference tracker and the receiver ran wirelessly with fully independent readout systems, and a positioning accuracy of 3.9 cm (RMS) has been achieved. We also evaluated the outcome of measuring continuous indoor localization of a moving receiver with this prototype. Our results indicated that further improvements in positioning accuracy will be attainable by acquiring higher angular resolution of the reference trackers. It is anticipated that "sub-cm level" navigation will be possible for muPS which could be applied to many situations such as future autonomous mobile robot operations.
Novel Identification Method for Demodex Viability in Human Eyes
Authors: Niu Qing , Cai Shiyuan , Hu Yuxuan , Geng Yuqian , Yang Cici , Yu Wenying , Liu Yi
Published: September 2023

Background
The current treatments cannot kill the mites, hence the need to evaluate optimal culture conditions for demodex mites in vitro and explore specific drugs for demodicosis.
Objective
Using a new method to identify the viability of Demodex folliculorum for screening clinically relevant drugs more accurately.
Methods
We compared the autofluorescence changes of demodex in a tea tree oil preparation (TTO preparation) and PBS buffer. Using the propidium fluorescent dye, the fluorescence intensity was measured using the Image-J software.
Results
In the two experimental groups, the diffusion speed of demodex's own blue fluorescence combined with joint ganglion in the PBS group was slower than that in the TTO preparation group, and the stability time of the whole body blue fluorescence was longer. The fluorescence peak value of PI stained red fluorescence was lower than that of TTO preparation group, and the staining fluorescence value required longer stability time, with lower fluorescence intensity at stabilization, which was consistent with expectations.
Limitations:
Fewer types of demodex culture media were selected for the experiment.
Conclusion
This novel demodex mite viability identification method objectively and effectively screens demodex-related drugs and can provide an effective reference for subsequent drug screening and evaluation.
Analysis of Piezoelectric materials to Fabricate ECG Sensor for Wearable Device
Authors: Sowmya S , Jose Deepa , Dudekula Khasim Vali , Mishra Sanket
Published: September 2023

The authors have requested that this preprint be removed from Research Square.