bluuebunny/crossref_metadata_2025 huggingface dataset:
Touching and being touched: where knowing and feeling meet
Authors: Fischman Lawrence
Published: July 2023

Philosophers maintain that touch confers a sense of reality or grounding to perceptual experience. In touching oneself, one is simultaneously both subject and object of touch, a template for experiencing oneself as subject and object of intentions, feelings, and motivations, or intersubjectivity. Here, I explore a form of self-touch carefully documented by Winnicott in observing how the infant engages the transitional object. I compare the processes of self-loss in transitional states, including absorption in art, empathic immersion, drug-induced ego dissolution, and depersonalization. I use examples drawn from Rodin, Dante, and the Beatles; research correlating neurophysiological findings with aspects of self-representation; predictive processing-based models; Hohwy’s concepts of minimal and narrative self; Clark’s notion of the extended mind; and phenomenological perspectives on touch, to postulate a role for self-touch in the pre-reflective sense of mine-ness, or grounding, in transitional states.
Residents’ participation in energy performance certification for collective action on climate change: the case of EnerGuide
Authors: Chen Xinyu , Gou Zhonghua , Zhang Huihua
Published: July 2023

Introduction

Although governments have launched energy performance certification (EPC) programs to increase residential energy efficiency, residents’ perception and acceptance of these programs have been little studied. This study contextualizes residents’ intentions to participate in EPC programs in terms of their attributions to and attitudes toward climate change to find mediating factors and effects to help trigger collective action to reduce residential sector energy demand.

Methods

This study employed a partial least squares structural equation modeling approach to empirically analyze a survey conducted on 400 residents of Edmonton, Canada, who participated in the Canadian energy efficiency rating and labeling program, EnerGuide.

Results and Discussion

Using EnerGuide, a Canadian energy efficiency rating and labeling program, as an example, this study establishes a framework to explain that local residents’ internal and external attributions to climate change elicit positive attitudes (need to take action), increasing their recognition of energy efficiency program benefits, which further promotes their EnerGuide program acceptance and participation intentions. This study also reveals the mediating effects between variables. Residents’ attitudes toward climate change mediate the relationship between internal/external attributions and EnerGuide program acceptance, and they indirectly impact residents’ program acceptance and participation intentions, with this effect moderated by energy efficiency program benefits. Residents’ program acceptance also mediates the relationship between climate change attitudes/energy efficiency program benefits and the intention outcome.

Implication

The study provides an example of the use of climate change discourse to motivate residents’ energy efficiency program participation.
Beyond physical health: the role of psychosocial challenges and stigma in tackling the COVID-19 pandemic—A scoping review
Authors: Jain Lakshit , Bhivandkar Siddhi , Baqir Huma , Shoib Sheikh , Nimavat Nirav , Mohan Anmol , Zubair Aarij Shakil , Jawad Muhammad Youshay , Muhammed Nazar , Ahmed Rizwan , Sachdeva Vishi , Ahmed Saeed
Published: July 2023

Background

The socio-cultural response to the Coronavirus Disease 2019 (COVID-19) and the level of adherence to evidence-based guidelines played a crucial role in determining the morbidity and mortality outcomes during the pandemic. This review aims to evaluate the impact of stigma and psycho-socio-cultural challenges on efforts to control the COVID-19 pandemic and to identify ways to mitigate such challenges in future pandemics.

Methods

Using keywords including COVID-19, coronavirus, stigma, psychosocial challenges, and others, the authors searched seven major databases with a time limitation of July 2021, which yielded 2,038 results. Out of these, 15 papers were included in this review.

Results

The findings of the review indicated that several psychosocial, socio-economic, and ethno-cultural factors are linked to the transmission and control of COVID-19. The research revealed that stigma and related psychosocial challenges and others, such as anxiety, fear, and stigma-driven social isolation, have resulted in significant mental health problems.

Discussion

The review underscores the negative impact of stigma on COVID-19 patients, survivors, and the general population. Addressing stigma and psychosocial challenges is crucial to effectively manage the current pandemic and to prevent similar challenges during future public health crises.
Because its power remains naturalized: introducing the settler colonial determinants of health
Authors: Wispelwey Bram , Tanous Osama , Asi Yara , Hammoudeh Weeam , Mills David
Published: July 2023

Indigenous people suffer earlier death and more frequent and severe disease than their settler counterparts, a remarkably persistent reality over time, across settler colonized geographies, and despite their ongoing resistance to elimination. Although these health inequities are well-known, they have been impervious to comprehensive and convincing explication, let alone remediation. Settler colonial studies, a fast-growing multidisciplinary and interdisciplinary field, is a promising candidate to rectify this impasse. Settler colonialism’s relationship to health inequity is at once obvious and incompletely described, a paradox arising from epistemic coloniality and perceived analytic challenges that we address here in three parts. First, in considering settler colonialism an enduring structure rather than a past event, and by wedding this fundamental insight to the ascendant structural paradigm for understanding health inequities, a picture emerges in which this system of power serves as a foundational and ongoing configuration determining social and political mechanisms that impose on human health. Second, because modern racialization has served to solidify and maintain the hierarchies of colonial relations, settler colonialism adds explanatory power to racism’s health impacts and potential amelioration by historicizing this process for differentially racialized groups. Finally, advances in structural racism methodologies and the work of a few visionary scholars have already begun to elucidate the possibilities for a body of literature linking settler colonialism and health, illuminating future research opportunities and pathways toward the decolonization required for health equity.
Do we react differently toward bionic devices vs. cochlear implants and wheelchairs? Possible links with personality traits
Authors: Oancea-Matei Diana-Alina , Gherguț Alois , Maftei Alexandra
Published: July 2023

The present study explored the attitudes toward individuals with bionic eyes and limbs, cochlear implants, and people with disabilities that imply using a wheelchair. Our sample consisted of 474 Romanian adults aged 18–61 (M = 27.56, SD = 11.80). Participants were randomly divided into five groups. They all filled scales related to personality characteristics, i.e., agreeableness, neuroticism, openness to experience, comprehension/intellectual efficiency, and previous contact with disability. Then, each group was presented with a vignette describing a character (wheelchair/bionic eye/bionic leg/cochlear implant/control group). Finally, they answered questions about their emotions, cognitions, and behaviors related to that context. Overall, our results suggested that higher agreeability, extraversion, openness to experience, intellectual complexity, and lower neuroticism were generally associated with more positive attitudes toward disability. When examining the differences in participants' emotions, cognitions, and behaviors depending on the target's characteristics, our results generally suggested that the most negative reactions were toward the character with a bionic eye. We discuss these findings considering their importance for shaping positive attitudes related to disability, especially related to the future technological advances in bionic devices.
The immunogenic radiation and new players in immunotherapy and targeted therapy for head and neck cancer
Authors: Sharon Shay , Daher-Ghanem Narmeen , Zaid Deema , Gough Michael J. , Kravchenko-Balasha Nataly
Published: July 2023

Although treatment modalities for head and neck cancer have evolved considerably over the past decades, survival rates have plateaued. The treatment options remained limited to definitive surgery, surgery followed by fractionated radiotherapy with optional chemotherapy, and a definitive combination of fractionated radiotherapy and chemotherapy. Lately, immunotherapy has been introduced as the fourth modality of treatment, mainly administered as a single checkpoint inhibitor for recurrent or metastatic disease. While other regimens and combinations of immunotherapy and targeted therapy are being tested in clinical trials, adapting the appropriate regimens to patients and predicting their outcomes have yet to reach the clinical setting. Radiotherapy is mainly regarded as a means to target cancer cells while minimizing the unwanted peripheral effect. Radiotherapy regimens and fractionation are designed to serve this purpose, while the systemic effect of radiation on the immune response is rarely considered a factor while designing treatment. To bridge this gap, this review will highlight the effect of radiotherapy on the tumor microenvironment locally, and the immune response systemically. We will review the methodology to identify potential targets for therapy in the tumor microenvironment and the scientific basis for combining targeted therapy and radiotherapy. We will describe a current experience in preclinical models to test these combinations and propose how challenges in this realm may be faced. We will review new players in targeted therapy and their utilization to drive immunogenic response against head and neck cancer. We will outline the factors contributing to head and neck cancer heterogeneity and their effect on the response to radiotherapy. We will review in-silico methods to decipher intertumoral and intratumoral heterogeneity and how these algorithms can predict treatment outcomes. We propose that (a) the sequence of surgery, radiotherapy, chemotherapy, and targeted therapy should be designed not only to annul cancer directly, but to prime the immune response. (b) Fractionation of radiotherapy and the extent of the irradiated field should facilitate systemic immunity to develop. (c) New players in targeted therapy should be evaluated in translational studies toward clinical trials. (d) Head and neck cancer treatment should be personalized according to patients and tumor-specific factors.
Overcome the fragmentation in online propaganda literature: the role of cultural and cognitive sociology
Authors: Nerino Valentina
Published: July 2023

Evidence concerning the proliferation of propaganda on social media has renewed scientific interest in persuasive communication practices, resulting in a thriving yet quite disconnected scholarship. This fragmentation poses a significant challenge, as the absence of a structured and comprehensive organization of this extensive literature hampers the interpretation of findings, thus jeopardizing the understanding of online propaganda functioning. To address this fragmentation, I propose a systematization approach that involves utilizing Druckman's Generalizing Persuasion Framework as a unified interpretative tool to organize this scholarly work. By means of this approach, it is possible to systematically identify the various strands within the field, detect their respective shortcomings, and formulate new strategies to bridge these research strands and advance our knowledge of how online propaganda operates. I conclude by arguing that these strategies should involve the sociocultural perspectives offered by cognitive and cultural sociology, as these provide important insights and research tools to disentangle and evaluate the role played by supra-individual factors in the production, distribution, consumption, and evaluation of online propaganda.
In vitro and in vivo evaluation of chlorhexidine salts as potential alternatives to potassium dichromate for Eimeria maxima M6 oocyst preservation
Authors: Laverty Lauren , Beer Lesleigh C. , Martin Kristen , Hernandez-Velasco Xochitl , Juarez-Estrada Marco A. , Arango-Cardona Marcela , Forga Aaron J. , Coles Makenly E. , Vuong Christine N. , Latorre Juan D. , Señas-Cuesta Roberto , Loeza Ileana , Gray Latasha S. , Barta John R. , Hargis Billy M. , Tellez-Isaias Guillermo , Graham Brittany D.
Published: July 2023

Introduction

Coccidiosis caused by the Eimeria spp., an Apicomplexan protozoon, is a major intestinal disease that affects the poultry industry. Although most cases of coccidiosis are subclinical, Eimeria infections impair bird health and decrease overall performance, which can result in compromised welfare and major economic losses. Viable sporulated Eimeria oocysts are required for challenge studies and live coccidiosis vaccines. Potassium dichromate (PDC) is typically used as a preservative for these stocks during storage. Although effective and inexpensive, PDC is also toxic and carcinogenic. Chlorhexidine (CHX) salts may be a possible alternative, as this is a widely used disinfectant with less toxicity and no known carcinogenic associations

Methods

In vitro testing of CHX gluconate and CHX digluconate exhibited comparable oocyst integrity and viability maintenance with equivalent bacteriostatic and bactericidal activity to PDC. Subsequent use of CHX gluconate or digluconate-preserved Eimeria oocysts, cold-stored at 4°C for 5 months, as the inoculum also resulted in similar oocyst shedding and recovery rates when compared to PDC-preserved oocysts.

Results and discussion

These data show that using 0.20% CHX gluconate could be a suitable replacement for PDC. Additionally, autofluorescence was used as a method to evaluate oocyst viability. Administration of artificially aged oocysts exhibiting >99% autofluorescence from each preserved treatment resulted in no oocyst output for CHX salt groups.
An Equivalent Form Related to a Hilbert-Type Integral Inequality
Authors: Rassias Michael Th. , Yang Bicheng , Raigorodskii Andrei
Published: July 2023

In the present paper, we establish an equivalent form related to a Hilbert-type integral inequality with a non-homogeneous kernel and a best possible constant factor. We also consider the case of homogeneous kernel as well as certain operator expressions.
Pathogenic SCN5A Mutation and Thyrotoxicosis-Related Neurological Syndrome: Casual or Causal Relationship?
Authors: Xu Yangqi , Zhao Lin , Dong Jihong , Jiang Jingjing , Jin Lirong
Published: July 2023

Background: Various neurologic complications of hyperthyroidism are reported, and most of these complications are reversible with the amelioration of thyrotoxicosis. We report a previously undescribed concurrence of hyperthyroid-associated exercise-induced myalgia and stiffness, pyramidal tract dysfunction, and myoclonic movements that make an initial clinical diagnosis difficult. Case presentation: A 17-year-old male was hospitalized in the department of neurology, presenting with a 4-year history of severe exercise-induced myalgia and stiffness, weakness of lower limbs, and myoclonic movements. Laboratory investigations unexpectedly revealed hyperthyroidism. MRI of the brain and spine, electrophysiology, and whole exome sequencing were also performed. Antithyroid therapy led to marked improvement of neurologic symptoms, accompanied by a significant improvement of the time-dependent decline in compound muscle action potentials (CMAP) amplitudes after exercise and normalization of the prolonged QTc interval. Genetic analysis identified a rare variant in SCN5A. Conclusion: This case report provides important insights into the relationship between hyperthyroidism and neurologic/cardiac complications, particularly in those with a genetic predisposition. SCN5A mutation possibly plays a role in the complex neurological syndrome associated with hyperthyroidism. Further studies are warranted to better understand the underlying mechanisms and potential therapeutic options for these complex conditions.
The Development and Role of Capmatinib in the Treatment of MET-Dysregulated Non-Small Cell Lung Cancer—A Narrative Review
Authors: Hsu Robert , Benjamin David J. , Nagasaka Misako
Published: July 2023

Non-small cell lung cancer (NSCLC) is a leading cause of death, but over the past decade, there has been tremendous progress in the field with new targeted therapies. The mesenchymal–epithelial transition factor (MET) proto-oncogene has been implicated in multiple solid tumors, including NSCLC, and dysregulation in NSCLC from MET can present most notably as MET exon 14 skipping mutation and amplification. From this, MET tyrosine kinase inhibitors (TKIs) have been developed to treat this dysregulation despite challenges with efficacy and reliable biomarkers. Capmatinib is a Type Ib MET TKI first discovered in 2011 and was FDA approved in August 2022 for advanced NSCLC with MET exon 14 skipping mutation. In this narrative review, we discuss preclinical and early-phase studies that led to the GEOMETRY mono-1 study, which showed beneficial efficacy in MET exon 14 skipping mutations, leading to FDA approval of capmatinib along with Foundation One CDx assay as its companion diagnostic assay. Current and future directions of capmatinib are focused on improving the efficacy, overcoming the resistance of capmatinib, and finding approaches for new indications of capmatinib such as acquired MET amplification from epidermal growth factor receptor (EGFR) TKI resistance. Clinical trials now involve combination therapy with capmatinib, including amivantamab, trametinib, and immunotherapy. Furthermore, new drug agents, particularly antibody–drug conjugates, are being developed to help treat patients with acquired resistance from capmatinib and other TKIs.
Technological Convergence: Highlighting the Power of CRISPR Single-Cell Perturbation Toolkit for Functional Interrogation of Enhancers
Authors: Ghamsari Reza , Rosenbluh Joseph , Menon A Vipin , Lovell Nigel H. , Alinejad-Rokny Hamid
Published: July 2023

Higher eukaryotic enhancers, as a major class of regulatory elements, play a crucial role in the regulation of gene expression. Over the last decade, the development of sequencing technologies has flooded researchers with transcriptome-phenotype data alongside emerging candidate regulatory elements. Since most methods can only provide hints about enhancer function, there have been attempts to develop experimental and computational approaches that can bridge the gap in the causal relationship between regulatory regions and phenotypes. The coupling of two state-of-the-art technologies, also referred to as crisprQTL, has emerged as a promising high-throughput toolkit for addressing this question. This review provides an overview of the importance of studying enhancers, the core molecular foundation of crisprQTL, and recent studies utilizing crisprQTL to interrogate enhancer-phenotype correlations. Additionally, we discuss computational methods currently employed for crisprQTL data analysis. We conclude by pointing out common challenges, making recommendations, and looking at future prospects, with the aim of providing researchers with an overview of crisprQTL as an important toolkit for studying enhancers.
Health Technology Reassessment: Addressing Uncertainty in Economic Evaluations of Oncology Drugs at Time of Reimbursement Using Long-Term Clinical Trial Data
Authors: Ball Graeme , Levine Mitchell A. H. , Thabane Lehana , Tarride Jean-Eric
Published: July 2023

The evidence base to support reimbursement decision making for oncology drugs is often based on short-term follow-up trial data, and attempts to address this uncertainty are not typically undertaken once a reimbursement decision is made. To address this gap, we sought to conduct a reassessment of an oncology drug (pembrolizumab) for patients with advanced melanoma which was approved based on interim data with a median 7.9 months of follow-up and for which long-term data have since been published. We developed a three-health-state partitioned survival model based on the phase 3 KEYNOTE-006 clinical trial data using patient-level data reconstruction techniques based on an interim analysis. We used a standard survival analysis and parametric curve fitting techniques to extrapolate beyond the trial follow-up time, and the model structure and inputs were derived from the literature. Five-year long-term follow-up data from the trial were then used to re-evaluate the cost-effectiveness of pembrolizumab versus ipilimumab for treatment of advanced melanoma. The best fitting parametric curves and corresponding survival extrapolations for reconstructed interim data and long-term data reconstructed from KEYNOTE-006 were different. An analysis of the 5 year long-term follow-up data generated a base case incremental cost-effectiveness ratio (ICER) that was 28% higher than the ICER based on interim trial data. Our findings suggest that there may be a trade-off between certainty and the ICER. Conducting health technology re-assessments of certain oncology products on the basis of longer-term data availability, especially for those health technology adoption decisions made based on immature clinical data, may be of value to decision makers.
Feeding and Reproductive Phenotypic Traits of the Sea Urchin Tripneustes gratilla in Seagrass Beds Impacted by Eutrophication
Authors: Bangi Helen Grace P. , Juinio-Meñez Marie Antonette
Published: July 2023

The sea urchin Tripneustes gratilla is a major grazer and is, hence, an excellent key model organism to study to gain a better understanding of responses to changes in its habitat. We investigated whether there are significant variations in the feeding and reproductive phenotypic traits of populations from three seagrass bed sites, with respect to their proximity to fish farms in Bolinao, northwestern Philippines. We established three stations in each of the three sites: the far, the intermediate, and those near the fish farms, and compared the sea urchins’ phenotypic traits and determined whether these were related to seagrass productivity and water parameters. Regardless of the sampling period, adult sea urchins (66.92 ± 0.27 mm test diameter, TD, n = 157) from the areas intermediate and near to the fish farms had significantly lower indices of Aristotle’s lantern, gut contents, gut and gonads, and lower gonad quality (high percentage of unusual black gonads), compared to those from the far stations. Multivariate analysis showed that the smaller feeding structures and gut, lower consumption rates and lower gonad indices and quality of sea urchins in the intermediate and near fish farms were positively related to lower shoot density, leaf production and species diversity, as well as lower water movement in those stations. The larger size of the Aristotle’s lantern in the far stations was not related to food limitations. More importantly, the phenotypic variability in the feeding structures and gonads of sea urchins in the same seagrass bed provides new evidence regarding the sensitivity of this species to environmental factors that may affect variability in food quality.
Ultrasound or MRI in the Evaluation of Anterior Talofibular Ligament (ATFL) Injuries: Systematic Review and Meta-Analysis
Authors: Colò Gabriele , Bignotti Bianca , Costa Giacomo , Signori Alessio , Tagliafico Alberto
Published: July 2023

Objectives: Ankle sprains represent the second most common cause of emergency department access for musculoskeletal injury and lateral ankle ligament complex tears account for 850,000 cases annually in the United States with a relapse rate of 70%. Clinical examination is limited due to its subjectivity and the difficulty of identifying a specific involvement of the ligament; therefore, US and MRI are frequently requested. Therefore, the goal of this study is to analyze the available literature on the use of ultrasound (US) and magnetic resonance imaging (MRI) to diagnose injuries to the anterior talofibular ligament (ATFL) with a meta-analytic approach. Methods: According to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) guidelines, all studies regarding the diagnostic accuracy of ultrasound and magnetic resonance imaging ATFL injuries were searched and assessed. The data were obtained from two independent reviewers with 12 and 3 years of experience in meta-analysis. A QUADAS-2 (Quality Assessment of Studies of Diagnostic Accuracy Studies) checklist was carried out to assess the risk of biases. From the selected studies, the sensitivity, specificity, and accuracy data were extracted. Results: Nine studies were included. The results of the meta-analysis demonstrate a greater sensitivity for ultrasound [96.88 (95% CI: 94–99) (fixed effects); 97 (95% CI: 94–99) (random effects)] compared to MRI [88.50 (95% CI: 85–91) (fixed effects); 86.98 (95% CI: 77–94) (random effects)], p < 0.05. The result of this meta-analysis shows that the less expensive diagnostic technique is also the most sensitive for the diagnosis of ATFL tears. Ultrasound articles resulted to have non-heterogeneity [(p = 0.2816; I° = 21.4607%)]. Conclusion: This meta-analysis demonstrates that US appears to be a highly sensitive diagnostic technique for diagnosing tears of the ATFL. Compared to MRI, the sensitivity of US result was higher.
Deriving Vocal Fold Oscillation Information from Recorded Voice Signals Using Models of Phonation
Authors: Zhao Wayne , Singh Rita
Published: July 2023

During phonation, the vocal folds exhibit a self-sustained oscillatory motion, which is influenced by the physical properties of the speaker’s vocal folds and driven by the balance of bio-mechanical and aerodynamic forces across the glottis. Subtle changes in the speaker’s physical state can affect voice production and alter these oscillatory patterns. Measuring these can be valuable in developing computational tools that analyze voice to infer the speaker’s state. Traditionally, vocal fold oscillations (VFOs) are measured directly using physical devices in clinical settings. In this paper, we propose a novel analysis-by-synthesis approach that allows us to infer the VFOs directly from recorded speech signals on an individualized, speaker-by-speaker basis. The approach, called the ADLES-VFT algorithm, is proposed in the context of a joint model that combines a phonation model (with a glottal flow waveform as the output) and a vocal tract acoustic wave propagation model such that the output of the joint model is an estimated waveform. The ADLES-VFT algorithm is a forward-backward algorithm which minimizes the error between the recorded waveform and the output of this joint model to estimate its parameters. Once estimated, these parameter values are used in conjunction with a phonation model to obtain its solutions. Since the parameters correlate with the physical properties of the vocal folds of the speaker, model solutions obtained using them represent the individualized VFOs for each speaker. The approach is flexible and can be applied to various phonation models. In addition to presenting the methodology, we show how the VFOs can be quantified from a dynamical systems perspective for classification purposes. Mathematical derivations are provided in an appendix for better readability.
Generalized Regression Neural Network Based Meta-Heuristic Algorithms for Parameter Identification of Proton Exchange Membrane Fuel Cell
Authors: He Peng , Zhou Xin , Liu Mingqun , Xu Kewei , Meng Xian , Yang Bo
Published: July 2023

An accurate parameter extraction of the proton exchange membrane fuel cell (PEMFC) is crucial for establishing a reliable cell model, which is also of great significance for subsequent research on the PEMFC. However, because the parameter identification of the PEMFC is a nonlinear optimization problem with multiple variables, peaks, and a strong coupling, it is difficult to solve this problem using traditional numerical methods. Furthermore, because of insufficient current and voltage data measured by the PEMFC, the precision rate of cell parameter extraction is also very low. The study proposes a parameter extraction method using a generalized regression neural network (GRNN) and meta-heuristic algorithms (MhAs). First of all, a GRNN is used to de-noise and predict the data to solve the problems in the field of PEMFC, which include insufficient data and excessive noise data of the measured data. After that, six typical algorithms are used to extract the parameters of the PEMFC under three operating conditions, namely high temperature and low pressure (HTLP), medium temperature and medium pressure (MTMP), and low temperature and high pressure (LTHP). The last results demonstrate that the application of GRNN can prominently decrease the influence of data noise on parameter identification, and after data prediction, it can greatly enhance the precision rate and reliability of MhAs parameter identification, specifically, under HTLP conditions, the V-I fitting accuracy achieved 99.39%, the fitting accuracy was 99.07% on MTMP, and the fitting accuracy was 98.70%.
Dissection of AT-Hook Motif Nuclear-Localized Genes and Their Potential Functions in Peach Growth and Development
Authors: Zhao Jianlun , Xu Enkai , Wang Qirui
Published: July 2023

The AT-hook motif nuclear-localized (AHL) family members play key roles in plant biological processes via protein–protein and protein-DNA interactions. Here, 22 non-redundant PpAHL genes were identified and analyzed in peach (Prunus persica), one of economically important non-timber forestry crops. The maximum-likelihood (ML) tree classified the PpAHLs into two clades (Clade-A and Clade-B) with three subfamilies: Type_I, Type_II, and Type_III. Exon–intron analysis exhibited that the PpAHLs from Type_I except one (Prupe.1G530300.1) lacked introns, and the PpAHLs from Type_II and Type_III gradually emerged with intron additions, indicating spatial expression patterns, evolutionarily distinct temporal patterns and, likely, neofunctionalization. Duplication event analysis suggested that PpAHLs in peach were mainly expanded through the large-scale duplication events. RNA-seq data showed that PpAHLs were induced by drought stress, and two genes (Prupe.1G530300.1 and Prupe.1G034400.1) from Type_I AHLs were induced at all time points, indicating that they might play key roles in the response to drought stress in peach. The tissue-specific expression pattern of PpAHLs exhibited their biological functions in the development of these specific tissues. In addition, the transient overexpression of Prupe.1G530300.1 and Prupe.1G034400.1 resulted in significant changes in sugar content, suggesting that they may be positive regulators of sugar accumulation in peach fruits. Our study provided novel insights into the roles of PpAHLs in plant development, which was helpful for the functional analysis of peach and related woody fruit trees, and for formulating new strategies for further breeding.
Genome-Wide Characterization and Gene Expression Analysis of TRP Channel Superfamily Genes in the Migratory Locust, Locusta migratoria
Authors: Yang Yong , Guo Wenhui , Wang Mingjun , Zhang Daochuan
Published: July 2023

The TRP channel superfamily was widely found in multiple species. They were involved in many extrasensory perceptions and were important for adapting to the environment. The migratory locust was one of the worldwide agricultural pests due to huge damage. In this study, we identified 13 TRP superfamily genes in the locust genome. The number of LmTRP superfamily genes was consistent with most insects. The phylogenetic tree showed that LmTRP superfamily genes could be divided into seven subfamilies. The conserved motifs and domains analysis documented that LmTRP superfamily genes contained unique characteristics of the TRP superfamily. The expression profiles in different organs identified LmTRP superfamily genes in the head and antennae, which were involved in sensory function. The expression pattern of different life phases also demonstrated that LmTRP superfamily genes were mainly expressed in third-instar nymphs and male adults. Our findings could contribute to a better understanding of the TRP channel superfamily gene and provide potential targets for insect control.
What Are the Characteristics of Torture Victims in Recently Arrived Refugees? A Cross-Sectional Study of Newly Arrived Refugees in Aarhus, Denmark
Authors: Hvidegaard Mette , Lanng Kamilla , Meyer Karin , Wejse Christian , Hvass Anne Mette Fløe
Published: July 2023

Torture victims live with complex health conditions. It is essential for the rehabilitation of torture survivors that their traumas are recognized at an early stage. The aim of this study was to investigate (i) the prevalence of reported torture exposure, (ii) the association between demographic characteristics and exposure to torture, and (iii) the association between PTSD and exposure to torture among recently arrived refugees in Aarhus, Denmark. Data were extracted from health assessments of refugees arriving in Aarhus in the years 2017–2019, and 208 cases were included in the analysis. The prevalence of reported torture was 13.9% (29/208). Most torture victims were found among refugees arriving from Iran (17.0% (9/53)), Syria (9.3% (8/86)), and Afghanistan (25.0% (5/20)). Significant associations were found between reported torture exposure and male gender, Southeast Asian origin, and a diagnosis of PTSD. In the study, 24.5% (24/98) of males and 4.5% (5/110) of females had been subjected to torture. However, it is possible that the prevalence of female torture survivors is underestimated due to the taboos surrounding sexual assaults and fear of stigmatization. Nearly half of the torture victims in the study were diagnosed with PTSD (44.8% (13/29)). The results confirm that torture victims constitute a vulnerable group living with severe consequences, including mental illness such as PTSD. Furthermore, understanding the cultural perspectives of the distress among refugees is crucial in providing appropriate healthcare services. This study highlights the importance of addressing the mental health needs of torture survivors and tailoring interventions toward vulnerable refugee populations.
Epigenomics Analysis of the Suppression Role of SIRT1 via H3K9 Deacetylation in Preadipocyte Differentiation
Authors: Yang Youzhualamu , Peng Wei , Su Xiaolong , Yue Binglin , Shu Shi , Wang Jikun , Fu Changqi , Zhong Jincheng , Wang Hui
Published: July 2023

Sirtuin 1 (SIRT1) overexpression significantly inhibits lipid deposition during yak intramuscular preadipocyte (YIMA) differentiation; however, the regulatory mechanism remains unknown. We elucidated the role of SIRT1 in YIMA differentiation using lentivirus-mediated downregulation technology and conducted mRNA-seq and ChIP-seq assays using H3K9ac antibodies after SIRT1 overexpression in order to reveal SIRT1 targets during YIMA adipogenesis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed in order to identify the functional annotation of common genes. In addition, a potential target of SIRT1 was selected to verify its effects on the differentiation and proliferation of YIMAs. SIRT1 interfered with lipid deposition and promoted YIMA differentiation. In total, 143,518 specific peaks were identified after SIRT1 overexpression, where genes associated with downregulation peaks were enriched in transcription, gene expression, lipid-related processes, and classical lipid-related pathways. The H3K9ac signal in the whole genome promoter region (2 kb upstream and downstream of the transcription start site (TSS)) was weakened, and the peaks were distributed across all gene functional regions. Genes that lost signals in their TSS region or gene body region were enriched in both biological processes and pathways associated with lipogenesis. The ChIP-seq results revealed 714 common differential genes in mRNA-seq, which were enriched in “MAPK signaling”, “lipid and atherosclerosis”, “mTOR signaling”, and “FoxO signaling” pathways. A total of 445 genes were downregulated in both their H3K9ac signals and mRNA expression, and one of their most significantly enriched pathways was FoxO signaling. Nine genes (FBP2, FPGT, HSD17B11, KCNJ15, MAP3K20, SLC5A3, TRIM23, ZCCHC10, and ZMYM1) lost the H3K9ac signal in their TSS regions and had low mRNA expression, and three genes (KCNJ15, TGM3, and TRIM54) had low expression but lost their H3K9ac signal in the gene body region. The interference of TRIM23 significantly inhibited fat deposition during preadipocyte differentiation and promoted cell proliferation by increasing S-phase cell numbers. The present study provides new insights into the molecular mechanism of intramuscular fat content deposition and the epigenetic role of SIRT1 in adipocyte differentiation.
Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Procambarus clarkii and Cambaroides dauricus from China
Authors: Luo Liang , Xu Yue , Wang Shihui , Zhang Rui , Guo Kun , Xu Wei , Zhao Zhigang
Published: July 2023

To enhance the management and protection of crayfish genetic diversity and germplasm resources in Cambaroides dauricus (C. dauricus), a common species of Procambarus clarkii (P. clarkii) was used as a control group to compare the whole mitochondrial genome sequence using Illumina sequencing technology. This study found that the mitochondrial genome of C. dauricus is 15580 bp in length, with a base composition of A (31.84%), G (17.66%), C (9.42%), and T (41.08%) and a C + G content of 27.08%. The C + G in the D-loop is rich in 17.06%, indicating a significant preference. The mitochondrial genome of C. dauricus contains 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes, with most of the genes labeled in the negative direction, except for a few genes that are labeled in the positive direction. The start codons of the ten coding sequences are ATG, and the quintessential TAA and TAG are the stop codons. This study also found that the Ka/Ks ratios of most protein-coding genes in the mitochondria of both shrimps are lower than 1, indicating weak natural selection, except for nad 2, nad 5, and cox 1. The Ka/Ks ratio of cox 3 is the lowest (less than 0.1), indicating that this protein-coding gene bears strong natural selection pressure and functional constraint in the process of mitochondrial genetic evolution of both shrimps. Furthermore, we constructed phylogenetic analyses based on the entire sequence, which effectively distinguishes the high body from other shrimp species of the genus based on the mitochondrial genome. This study provides molecular genetic data for the diversity investigation and protection of fishery resources with Chinese characteristics and a scientific reference for the evolutionary study of Procambarus.
Melanin’s Journey from Melanocytes to Keratinocytes: Uncovering the Molecular Mechanisms of Melanin Transfer and Processing
Authors: Bento-Lopes Liliana , Cabaço Luís C. , Charneca João , Neto Matilde V. , Seabra Miguel C. , Barral Duarte C.
Published: July 2023

Skin pigmentation ensures efficient photoprotection and relies on the pigment melanin, which is produced by epidermal melanocytes and transferred to surrounding keratinocytes. While the molecular mechanisms of melanin synthesis and transport in melanocytes are now well characterized, much less is known about melanin transfer and processing within keratinocytes. Over the past few decades, distinct models have been proposed to explain how melanin transfer occurs at the cellular and molecular levels. However, this remains a debated topic, as up to four different models have been proposed, with evidence presented supporting each. Here, we review the current knowledge on the regulation of melanin exocytosis, internalization, processing, and polarization. Regarding the different transfer models, we discuss how these might co-exist to regulate skin pigmentation under different conditions, i.e., constitutive and facultative skin pigmentation or physiological and pathological conditions. Moreover, we discuss recent evidence that sheds light on the regulation of melanin exocytosis by melanocytes and internalization by keratinocytes, as well as how melanin is stored within these cells in a compartment that we propose be named the melanokerasome. Finally, we review the state of the art on the molecular mechanisms that lead to melanokerasome positioning above the nuclei of keratinocytes, forming supranuclear caps that shield the nuclear DNA from UV radiation. Thus, we provide a comprehensive overview of the current knowledge on the molecular mechanisms regulating skin pigmentation, from melanin exocytosis by melanocytes and internalization by keratinocytes to processing and polarization within keratinocytes. A better knowledge of these molecular mechanisms will clarify long-lasting questions in the field that are crucial for the understanding of skin pigmentation and can shed light on fundamental aspects of organelle biology. Ultimately, this knowledge can lead to novel therapeutic strategies to treat hypo- or hyper-pigmentation disorders, which have a high socio-economic burden on patients and healthcare systems worldwide, as well as cosmetic applications.
Synthetic Thioesters of Thiamine: Promising Tools for Slowing Progression of Neurodegenerative Diseases
Authors: Bettendorff Lucien
Published: July 2023

Thiamine (vitamin B1) is essential for the brain. This is attributed to the coenzyme role of thiamine diphosphate (ThDP) in glucose and energy metabolism. The synthetic thiamine prodrug, the thioester benfotiamine (BFT), has been extensively studied and has beneficial effects both in rodent models of neurodegeneration and in human clinical studies. BFT has no known adverse effects and improves cognitive outcomes in patients with mild Alzheimer’s disease. In cell culture and animal models, BFT has antioxidant and anti-inflammatory properties that seem to be mediated by a mechanism independent of the coenzyme function of ThDP. Recent in vitro studies show that another thiamine thioester, O,S-dibenzoylthiamine (DBT), is even more efficient than BFT, especially with respect to its anti-inflammatory potency, and is effective at lower concentrations. Thiamine thioesters have pleiotropic properties linked to an increase in circulating thiamine concentrations and possibly in hitherto unidentified open thiazole ring derivatives. The identification of the active neuroprotective metabolites and the clarification of their mechanism of action open extremely promising perspectives in the field of neurodegenerative, neurodevelopmental, and psychiatric conditions. The present review aims to summarize existing data on the neuroprotective effects of thiamine thioesters and give a comprehensive account.
On Isotropy of Multimodal Embeddings
Authors: Tyshchuk Kirill , Karpikova Polina , Spiridonov Andrew , Prutianova Anastasiia , Razzhigaev Anton , Panchenko Alexander
Published: July 2023

Embeddings, i.e., vector representations of objects, such as texts, images, or graphs, play a key role in deep learning methodologies nowadays. Prior research has shown the importance of analyzing the isotropy of textual embeddings for transformer-based text encoders, such as the BERT model. Anisotropic word embeddings do not use the entire space, instead concentrating on a narrow cone in such a pretrained vector space, negatively affecting the performance of applications, such as textual semantic similarity. Transforming a vector space to optimize isotropy has been shown to be beneficial for improving performance in text processing tasks. This paper is the first comprehensive investigation of the distribution of multimodal embeddings using the example of OpenAI’s CLIP pretrained model. We aimed to deepen the understanding of the embedding space of multimodal embeddings, which has previously been unexplored in this respect, and study the impact on various end tasks. Our initial efforts were focused on measuring the alignment of image and text embedding distributions, with an emphasis on their isotropic properties. In addition, we evaluated several gradient-free approaches to enhance these properties, establishing their efficiency in improving the isotropy/alignment of the embeddings and, in certain cases, the zero-shot classification accuracy. Significantly, our analysis revealed that both CLIP and BERT models yielded embeddings situated within a cone immediately after initialization and preceding training. However, they were mostly isotropic in the local sense. We further extended our investigation to the structure of multilingual CLIP text embeddings, confirming that the observed characteristics were language-independent. By computing the few-shot classification accuracy and point-cloud metrics, we provide evidence of a strong correlation among multilingual embeddings. Embeddings transformation using the methods described in this article makes it easier to visualize embeddings. At the same time, multiple experiments that we conducted showed that, in regard to the transformed embeddings, the downstream tasks performance does not drop substantially (and sometimes is even improved). This means that one could obtain an easily visualizable embedding space, without substantially losing the quality of downstream tasks.
The Eight Unanswered and Answered Questions about the Use of Vasopressors in Septic Shock
Authors: Hamzaoui Olfa , Goury Antoine , Teboul Jean-Louis
Published: July 2023

Septic shock is mainly characterized—in addition to hypovolemia—by vasoplegia as a consequence of a release of inflammatory mediators. Systemic vasodilatation due to depressed vascular tone results in arterial hypotension, which induces or worsens organ hypoperfusion. Accordingly, vasopressor therapy is mandatory to correct hypotension and to reverse organ perfusion due to hypotension. Currently, two vasopressors are recommended to be used, norepinephrine and vasopressin. Norepinephrine, an α1-agonist agent, is the first-line vasopressor. Vasopressin is suggested to be added to norepinephrine in cases of inadequate mean arterial pressure instead of escalating the doses of norepinephrine. However, some questions about the bedside use of these vasopressors remain. Some of these questions have been well answered, some of them not clearly addressed, and some others not yet answered. Regarding norepinephrine, we firstly reviewed the arguments in favor of the choice of norepinephrine as a first-line vasopressor. Secondly, we detailed the arguments found in the recent literature in favor of an early introduction of norepinephrine. Thirdly, we reviewed the literature referring to the issue of titrating the doses of norepinephrine using an individualized resuscitation target, and finally, we addressed the issue of escalation of doses in case of refractory shock, a remaining unanswered question. For vasopressin, we reviewed the rationale for adding vasopressin to norepinephrine. Then, we discussed the optimal time for vasopressin administration. Subsequently, we addressed the issue of the optimal vasopressin dose, and finally we discussed the best strategy to wean these two vasopressors when combined.