bluuebunny/crossref_metadata_2025 huggingface dataset:
Residual Humidity in Paraffin-Embedded Tissue Reduces Nucleic Acid Stability
Authors: Abuja Peter M. , Pabst Daniela , Bourgeois Benjamin , Loibner Martina , Ulz Christine , Kufferath Iris , Fackelmann Ulrike , Stumptner Cornelia , Kraemer Rainer , Madl Tobias , Zatloukal Kurt
Published: April 2023

Molecular diagnostics in healthcare relies increasingly on genomic and transcriptomic methodologies and requires appropriate tissue specimens from which nucleic acids (NA) of sufficiently high quality can be obtained. Besides the duration of ischemia and fixation type, NA quality depends on a variety of other pre-analytical parameters, such as storage conditions and duration. It has been discussed that the improper dehydration of tissue during processing influences the quality of NAs and the shelf life of fixed tissue. Here, we report on establishing a method for determining the amount of residual water in fixed, paraffin-embedded tissue (fixed by neutral buffered formalin or a non-crosslinking fixative) and its correlation to the performance of NAs in quantitative real-time polymerase chain reaction (qRT-PCR) analyses. The amount of residual water depended primarily on the fixative type and the dehydration protocol and, to a lesser extent, on storage conditions and time. Moreover, we found that these parameters were associated with the qRT-PCR performance of extracted NAs. Besides the cross-linking of NAs and the modification of nucleobases by formalin, the hydrolysis of NAs by residual water was found to contribute to reduced qRT-PCR performance. The negative effects of residual water on NA stability are not only important for the design and interpretation of research but must also be taken into account in clinical diagnostics where the reanalysis of archived tissue from a primary tumor may be required (e.g., after disease recurrence). We conclude that improving the shelf life of fixed tissue requires meticulous dehydration and dry storage to minimize the degradative influence of residual water on NAs.
Transcriptomic and Functional Analyses Reveal the Different Roles of Vitamins C, E, and K in Regulating Viral Infections in Maize
Authors: Hao Kaiqiang , Yang Miaoren , Cui Yakun , Jiao Zhiyuan , Gao Xinran , Du Zhichao , Wang Zhiping , An Mengnan , Xia Zihao , Wu Yuanhua
Published: April 2023

Maize lethal necrosis (MLN), one of the most important maize viral diseases, is caused by maize chlorotic mottle virus (MCMV) infection in combination with a potyvirid, such as sugarcane mosaic virus (SCMV). However, the resistance mechanism of maize to MLN remains largely unknown. In this study, we obtained isoform expression profiles of maize after SCMV and MCMV single and synergistic infection (S + M) via comparative analysis of SMRT- and Illumina-based RNA sequencing. A total of 15,508, 7567, and 2378 differentially expressed isoforms (DEIs) were identified in S + M, MCMV, and SCMV libraries, which were primarily involved in photosynthesis, reactive oxygen species (ROS) scavenging, and some pathways related to disease resistance. The results of virus-induced gene silencing (VIGS) assays revealed that silencing of a vitamin C biosynthesis-related gene, ZmGalDH or ZmAPX1, promoted viral infections, while silencing ZmTAT or ZmNQO1, the gene involved in vitamin E or K biosynthesis, inhibited MCMV and S + M infections, likely by regulating the expressions of pathogenesis-related (PR) genes. Moreover, the relationship between viral infections and expression of the above four genes in ten maize inbred lines was determined. We further demonstrated that the exogenous application of vitamin C could effectively suppress viral infections, while vitamins E and K promoted MCMV infection. These findings provide novel insights into the gene regulatory networks of maize in response to MLN, and the roles of vitamins C, E, and K in conditioning viral infections in maize.
Multi-Omics Research Strategies for Psoriasis and Atopic Dermatitis
Authors: Guo Youming , Luo Lingling , Zhu Jing , Li Chengrang
Published: April 2023

Psoriasis and atopic dermatitis (AD) are multifactorial and heterogeneous inflammatory skin diseases, while years of research have yielded no cure, and the costs associated with caring for people suffering from psoriasis and AD are a huge burden on society. Integrating several omics datasets will enable coordinate-based simultaneous analysis of hundreds of genes, RNAs, chromatins, proteins, and metabolites in particular cells, revealing networks of links between various molecular levels. In this review, we discuss the latest developments in the fields of genomes, transcriptomics, proteomics, and metabolomics and discuss how they were used to identify biomarkers and understand the main pathogenic mechanisms underlying these diseases. Finally, we outline strategies for achieving multi-omics integration and how integrative omics and systems biology can advance our knowledge of, and ability to treat, psoriasis and AD.
Cadmium Absorption in Various Genotypes of Rice under Cadmium Stress
Authors: Feng Kaixuan , Li Jiangxia , Yang Yachun , Li Zhong , Wu Wenge
Published: April 2023

Cadmium (Cd) is a kind of heavy metal. Cadmium pollution in paddy fields will accumulate a large amount of cadmium in rice, which will affect the growth and development of rice. In addition, long-term consumption of rice contaminated with Cd can harm human health. In this study, four rice varieties with high Cd accumulation (S4699, TLY619, JHY1586, QLY155) and four varieties with low Cd accumulation (YY4949, CYJ-7, G8YXSM, MXZ-2) were screened through field experiments for two consecutive years (2021 and 2022) and differences in antioxidant enzyme systems and expression of genes in their organs were analyzed. The total Cd content showed as follows: indica rice > japonica rice, high-Cd-accumulation variety > low-Cd-accumulation variety, and the total Cd content of each organ of rice showed root > stem > leaf > grain. The results of the antioxidant enzyme system showed that the contents of malondialdehyde (MAD), reduced glutathione (GSH), oxidized glutathione (GSSH), and peroxidase (POD) were positively correlated with the total Cd content in rice, and superoxide dismutase (SOD) showed the opposite performance in the leaves. There was no correlation between catalase (CAT) and Cd content, but CAT content decreased in leaves and grains and increased in roots and stems with increasing fertility. Based on this study, RT-qPCR was used to further validate the expression of Cd-uptake-related genes in different rice varieties. It was found that high expression of OsHMA3, OsCCX2, OsNRAMP5, and OsHMA9 genes promoted Cd uptake and translocation in rice, especially in rice varieties with high Cd accumulation. The high expression of OslRT1, OsPCR1, and OsMTP1 genes hindered Cd uptake by rice plants, which was especially evident in low-accumulating Cd rice varieties. These results provide an important theoretical reference and scientific basis for our in-depth study and understanding of the mechanism of cadmium stress tolerance in rice.
Novel Strategy in Searching for Natural Compounds with Anti-Aging and Rejuvenating Potential
Authors: Koptyug Andrey , Sukhovei Yurij , Kostolomova Elena , Unger Irina , Kozlov Vladimir
Published: April 2023

We suggest a novel approach for searching natural compounds with anti-aging and rejuvenation potential using cell cultures, with a high potential for the further in vivo applications. The present paper discusses ways of defining age for cell populations with large numbers of cells and suggests a method of assessing how young or old a cell population is based on a cell age profile approach. This approach uses experimental distributions of the cells over the cell cycle stages, acquired using flow cytometry. This paper discusses how such a profile should evolve under homeostatic maintenance of cell numbers in the proliferation niches. We describe promising results from experiments on a commercial substance claiming rejuvenating and anti-aging activity acting upon the cultures of human mononuclear cells and dermal fibroblasts. The chosen substance promotes a shift towards larger proportion of cells in synthesis and proliferation stages, and increases cell culture longevity. Further, we describe promising in vivo testing results of a selected food supplement. Based on the described concept of cell age profile and available test results, a strategy to search for natural compounds with regenerative, anti-aging and rejuvenation potential is suggested and proposed for wider and thorough testing. Proposed methodology of age assessment is rather generic and can be used for quantitative assessment of the anti-aging and rejuvenation potential of different interventions. Further research aimed at the tests of the suggested strategy using more substances and different interventions, and the thorough studies of molecular mechanisms related to the action of the substance used for testing the suggested search methodology, are needed.
8-Methoxyflindersine-Induced Apoptosis and Cell Cycle Disorder Involving MAPK Signaling Activation in Human Colorectal Cancer Cells
Authors: Zhang Dianbao , Fu Yunmei , Liu Ying , Wu Yifan , Chen Jiayu , Zhang Luting , Wang Rui , Chen Zaixing , Liu Tao
Published: April 2023

Colorectal cancer (CRC) is one of the most common malignant tumors with a high lethal rate globally, and novel strategies for its prevention and therapy are urgently needed. In our previous work, 8-methoxyflindersine (8-MF), a quinoline alkaloid, was isolated from the Dictamni cortex, and its bioactivities were largely unknown. In this study, we found that 8-MF significantly inhibited cell viability in the CRC cell lines LoVo and RKO. The 8-MF-induced CRC cell apoptosis, as well as cell cycle disorder, were further verified by cyclins dysregulation in mRNA and protein levels. Further, the activation of MAPK family members p38 and ERK1/2 was observed after 8-MF treatment. Moreover, the protein–protein interaction of 8-MF with cyclins and MAPKs was demonstrated using the STRING database. The 8-MF could bind to p38 and ERK1/2 proteins in molecular docking. Taken together, we found that 8-MF induced apoptosis and cell cycle disorder involving MAPK signaling activation in CRC cells, indicating 8-MF as a novel lead compound candidate for the development of anti-tumor drugs for CRC.
Metabolic Disturbance of High-Saturated Fatty Acid Diet in Cognitive Preservation
Authors: Rivas-Domínguez Antonio , Mohamed-Mohamed Himan , Jimenez-Palomares Margarita , García-Morales Victoria , Martinez-Lopez Laura , Orta Manuel Luis , Ramos-Rodriguez Juan José , Bermudez-Pulgarin Beatriz
Published: April 2023

Aging continues to be the main cause of the development of Alzheimer’s, although it has been described that certain chronic inflammatory pathologies can negatively influence the progress of dementia, including obesity and hyperlipidemia. In this sense, previous studies have shown a relationship between low-density lipoprotein receptor (LDLR) and the amyloid-beta (Aβ) binding activity, one of the main neuropathological features of Alzheimer’s disease (AD). LDLR is involved in several processes, including lipid transport, regulation of inflammatory response and lipid metabolism. From this perspective, LDLR−/− mice are a widely accepted animal model for the study of pathologies associated with alterations in lipid metabolism, such as familial hypercholesterolemia, cardiovascular diseases, metabolic syndrome, or early cognitive decline. In this context, we induced hyperlipidemia in LDLR−/− mice after feeding with a high-saturated fatty acid diet (HFD) for 44 weeks. LDLR−/−-HFD mice exhibited obesity, hypertriglyceridemia, higher glucose levels, and early hepatic steatosis. In addition, HFD increased plasmatic APOE and ubiquitin 60S levels. These proteins are related to neuronal integrity and health maintenance. In agreement, we detected mild cognitive dysfunctions in mice fed with HFD, whereas LDLR−/−-HFD mice showed a more severe and evident affectation. Our data suggest central nervous system dysfunction is associated with a well-established metabolic syndrome. As a late consequence, metabolic syndrome boots many behavioral and pathological alterations recognized in dementia, supporting that the control of metabolic parameters could improve cognitive preservation and prognosis.
LARF: Two-Level Attention-Based Random Forests with a Mixture of Contamination Models
Authors: Konstantinov Andrei , Utkin Lev , Muliukha Vladimir
Published: April 2023

This paper provides new models of the attention-based random forests called LARF (leaf attention-based random forest). The first idea behind the models is to introduce a two-level attention, where one of the levels is the “leaf” attention, and the attention mechanism is applied to every leaf of trees. The second level is the tree attention depending on the “leaf” attention. The second idea is to replace the softmax operation in the attention with the weighted sum of the softmax operations with different parameters. It is implemented by applying a mixture of Huber’s contamination models and can be regarded as an analog of the multi-head attention, with “heads” defined by selecting a value of the softmax parameter. Attention parameters are simply trained by solving the quadratic optimization problem. To simplify the tuning process of the models, it is proposed to convert the tuning contamination parameters into trainable parameters and to compute them by solving the quadratic optimization problem. Many numerical experiments with real datasets are performed for studying LARFs. The code of the proposed algorithms is available.
A BIM-Based Approach for Pavement Monitoring Integrating Data from Non-Destructive Testing Methods (NDTs)
Authors: Bertolini Luca , D’Amico Fabrizio , Napolitano Antonio , Bianchini Ciampoli Luca , Gagliardi Valerio , Romer Diezmos Manalo Jhon
Published: April 2023

Monitoring of critical civil engineering infrastructures has become a priority for public owners and administrative authorities. Several laws and regulations have been issued on this topic, emphasizing the crucial role of Building Information Modeling (BIM)- based procedures for the design and management of civil infrastructures. This study aims at examining the potential of an interoperable and upgradeable BIM model supplemented by ground-based non-destructive survey data, such as Mobile Laser Scanner (MLS) and Ground-Penetrating Radar (GPR), for the analysis of the potential distresses identified in a transport infrastructure’s pavement. The main goal of the work is to implement an infrastructure management process that aims to reduce the limits associated with the separate observation of these assessments and to provide a more efficient way to store data regarding the status of a linear transport infrastructure, to the advantage of an integrated analysis. As on-site surveys are carried out, preliminary analyses on the condition of the inspected infrastructure are performed by relying on the information provided by Non-Destructive Testing (NDTs) inspections. Subsequently, a digital informative model capable of storing the data obtained by the surveys is generated, integrating both the MLS and GPR information to accurately represent the status of the infrastructure’s pavement in a three-dimensional environment. Data obtained from these instruments were used as the input for the digitalization process, making use of parametric digital elements capable of adapting their configuration to the information provided by the NDT surveys. As more analysis on the surveys’ results is carried out, potential distresses in the deep layers of the pavement are identified, and the information related to these elements is then integrated into the BIM model previously created. The process hereby described allows for an analysis of the three-dimensional configuration of the pavement, along with potential distresses and their location into the road’s superstructure. This digitalization process has shown promising viability for data management aimed at supporting asset managers in various management phases.
Cure of Alzheimer’s Dementia in Many Patients by Using Intranasal Insulin to Augment an Inadequate Counter-Reaction, Edaravone to Scavenge ROS, and 1 or 2 Other Drugs to Address Affected Brain Cells
Authors: Fessel Jeffrey
Published: April 2023

The goal of treatment for Alzheimer’s dementia (AD) is the restoration of normal cognition. No drug regimen has ever achieved this. This article suggests that curing AD may be achieved by combination therapy as follows. First, with intranasal insulin to augment the body’s natural counter-reaction to the changes in brain cell-types that produced the dementia. Second, with edaravone to decrease free radicals, which are increased and causal in AD. Third, as described elsewhere, with one or two drugs from among pioglitazone, fluoxetine, and lithium, which address the brain cell-types whose changed functions cause the dementia. Insulin restores cerebral glucose, which is the main nutrient for brain neurons whose depletion is responsible for the dementia; and edaravone decreases ROS, which are intrinsic causes of neuropathology in AD. This combination of drugs is a potential cure for many patients with AD, and should be tested in a clinical trial.
Congenital Ventricular Diverticulum
Authors: Rao Carmelo Massimiliano , Lucà Fabiana , Franzutti Claudio , Scappatura Giuseppe , Arcadi Nicola , Fratto Pasquale , Benedetto Francesco Antonio , Gelsomino Sandro
Published: April 2023

Herein, we describe a 54-year-old patient with a congenital ventricular diverticulum (CVD), referred to our emergency department for presyncope episodes and multiple re-entrant ventricular tachycardias (VT). Significantly, echocardiographic findings were not clear, and the diagnosis was made by cardiac magnetic resonance imaging (CMRI), which showed the presence of an apical accessory cavity connected to the ventricle and contracting synchronously. CMRI allowed the differential diagnosis with other outpouching cardiac defects. The patient underwent a subcutaneous implantable cardioverter defibrillator (S-ICD) implant and was referred for heart transplantation (HT). The diagnosis, treatment, and main findings of the CVD are discussed in this case report.
Comparison of Patient-Controlled versus Continuous Epidural Analgesia in Adult Surgical Patients: A Systematic Review
Authors: van Samkar Ganapathy , Ru Tan Yan , Hermanns Henning , Preckel Benedikt , Jamaludin Faridi S. , Hollmann Markus W. , Stevens Markus F.
Published: April 2023

Background: The advantages of PCEA over CEA have been demonstrated in obstetric patients. Whether a similar benefit applies to surgical patients is unclear. Methods: Embase, PubMed, and Cochrane Library were searched, enabling a systematic review of studies comparing PCEA and CEA in adult surgical patients (PROSPERO: CRD42018106644). The study quality was assessed using the Cochrane risk-of-bias tool (RoB2). The primary outcome was pain scores on postoperative day one (POD1). Secondary outcomes were 24 or 48 h epidural or intravenous total analgesic dose, systemic analgesics, manual top-ups, side effects, and patient satisfaction. Results: Six randomized controlled trials with high heterogeneity of study characteristics were identified with a moderate risk of bias. Two studies showed significantly reduced resting pain scores on POD1 in PCEA compared with CEA patients (36–44%, p < 0.05). Four studies found comparable pain scores between these groups. PCEA use reduced epidural medication (28% to 40% reduction, p < 0.01) in four studies. One study found a 23% reduction (p < 0.001) of top-ups in PCEA; intravenous morphine use by PCEA patients was reduced (0.16 vs. 3.45 mg per patient, p < 0.05) in one study. PCEA patients were more satisfied with analgesia (p < 0.001) in two studies. Nausea and vomiting were reduced in PCEA (p = 0.01). Conclusions: Regarding the reduction in pain scores, the effects of PCEA were not significant or clinically not relevant. However, regarding the amount of epidural drug use, the amount of required rescue systemic analgesics, patient satisfaction, and the number of required top-ups, PCEA had advantages over CEA in surgical patients.
Inter-Observer and Intra-Observer Reliability Assessment of the Established Classification Systems for Periprosthetic Shoulder Fractures
Authors: Wiethölter Mats , Akgün Doruk , Plachel Fabian , Minkus Marvin , Karczewski Daniel , Braun Karl , Thiele Kathi , Becker Luis , Stöckle Ulrich , Moroder Philipp
Published: April 2023

This study evaluated the reliability and comprehensiveness of the Unified classification system (UCPF), Wright & Cofield, Worland and Kirchhoff classifications and related treatment recommendations for periprosthetic shoulder fractures (PPSFx). Two shoulder arthroplasty specialists (experts) and two orthopaedic residents (non-experts) assessed 20 humeral-sided and five scapula-sided cases of PPSFx. We used the unweighted Cohen’s Kappa (κ) for measuring the intra-observer reliability and Krippendorff’s alpha (α) for measuring the inter-observer reliability. The inter-rater reliabilities for the Wright & Cofield and Worland classifications were substantial for all groups. The expert and non-expert groups for UCPF also showed substantial inter-rater agreement. The all-rater group for the UCPF and the expert and non-expert group for the Kirchhoff classification revealed moderate inter-rater reliability. For the Kirchhoff classification, only fair inter-rater reliability was found for the non-expert group. Almost perfect intra-rater reliability was measured for all groups of the Wright & Cofield classification and the all-rater and expert groups of the UCPF. All groups of the Kirchhoff and Worland classifications and the group of non-experts for the UCPF had substantial intra-rater reliabilities. Regarding treatment recommendations, substantial inter-rater and moderate intra-rater reliabilities were found. Simple classification systems for PPSFx (Wright & Cofield, Worland) show the highest inter- and intra-observer reliability but lack comprehensiveness as they fail to describe scapula-sided fractures. The complex Kirchhoff classification shows limited reliability. The UCPF seems to offer an acceptable combination of comprehensiveness and reliability.
Risk Factors and Role of Antibiotic Prophylaxis for Wound Infections after Percutaneous Endoscopic Gastrostomy
Authors: Mondorf Antonia , Amini Clara , Graf Christiana , Michael Florian Alexander , Blumenstein Irina , Jung Michael , Friedrich-Rust Mireen , Hack Daniel , Besier Silke M. , Hogardt Michael , Kempf Volkhard A. J. , Zeuzem Stefan , Welsch Christoph , Bojunga Jörg
Published: April 2023

Background and study aim: The incidence of wound infections after percutaneous endoscopic gastrostomy (PEG) varies widely in recent studies. The present study systematically investigates the underlying risk factors for the development of wound infections in a large cohort of patients over a long-term follow-up period. Patients and Methods: A retrospective cohort study of patients undergoing PEG insertion using either the pull or push technique was conducted and patients followed up for 3 years. Tube-related wound infections were identified, and pathogens regularly cultured from wound swabs. Adjusted analysis was performed via univariate and multivariate logistic regression analysis. Results: 616 patients were included in this study. A total of 25% percent of patients developed wound infections upon PEG tube insertion and 6.5% showed recurrent infections. Nicotine abuse (p = 0.01), previous ischemic stroke (p = 0.01) and head and neck cancer (p < 0.001) showed an increased risk for wound infection after PEG placement. Moreover, radio-chemotherapy was associated with the occurrence of wound infections (p < 0.001). Infection rates were similar between pull and push cohorts. The most common bacterial pathogen detected was Enterobacterales (19.2%). Staphylococcus aureus, Pseudomonas aeruginosa and enterococci were frequently detected in recurrent infection (14.2%, 11.4% and 9.6%, respectively). Antibiotic prophylaxis showed no effect on infection rates. Conclusions: Wound infections after PEG placement are common and occasionally occur as recurrent infections. There is potential for improvement in everyday clinical practice, particularly regarding antibiotic prophylaxis in accordance with guidelines.
Widespread Intra- and Inter-Network Dysconnectivity among Large-Scale Resting State Networks in Schizophrenia
Authors: Rong Bei , Huang Huan , Gao Guoqing , Sun Limin , Zhou Yuan , Xiao Ling , Wang Huiling , Wang Gaohua
Published: April 2023

Schizophrenia is characterized by the distributed dysconnectivity of resting-state multiple brain networks. However, the abnormalities of intra- and inter-network functional connectivity (FC) in schizophrenia and its relationship to symptoms remain unknown. The aim of the present study is to compare the intra- and inter-connectivity of the intrinsic networks between a large sample of patients with schizophrenia and healthy controls. Using the Region of interest (ROI) to ROI FC analyses, the intra- and inter-network FC of the eight resting state networks [default mode network (DMN); salience network (SN); frontoparietal network (FPN); dorsal attention network (DAN); language network (LN); visual network (VN); sensorimotor network (SMN); and cerebellar network (CN)] were investigated in 196 schizophrenia and 169-healthy controls. Compared to the healthy control group, the schizophrenia group exhibited increased intra-network FC in the DMN and decreased intra-network FC in the CN. Additionally, the schizophrenia group showed the decreased inter-network FC mainly involved the SN-DMN, SN-LN and SN-CN while increased inter-network FC in the SN-SMN and SN-DAN (p < 0.05, FDR-corrected). Our study suggests widespread intra- and inter-network dysconnectivity among large-scale RSNs in schizophrenia, mainly involving the DMN, SN and SMN, which may further contribute to the dysconnectivity hypothesis of schizophrenia.
Long-Term Effects of SARS-CoV-2 in the Brain: Clinical Consequences and Molecular Mechanisms
Authors: Granholm Ann-Charlotte
Published: April 2023

Numerous investigations have demonstrated significant and long-lasting neurological manifestations of COVID-19. It has been suggested that as many as four out of five patients who sustained COVID-19 will show one or several neurological symptoms that can last months after the infection has run its course. Neurological symptoms are most common in people who are less than 60 years of age, while encephalopathy is more common in those over 60. Biological mechanisms for these neurological symptoms need to be investigated and may include both direct and indirect effects of the virus on the brain and spinal cord. Individuals with Alzheimer’s disease (AD) and related dementia, as well as persons with Down syndrome (DS), are especially vulnerable to COVID-19, but the biological reasons for this are not clear. Investigating the neurological consequences of COVID-19 is an urgent emerging medical need, since close to 700 million people worldwide have now had COVID-19 at least once. It is likely that there will be a new burden on healthcare and the economy dealing with the long-term neurological consequences of severe SARS-CoV-2 infections and long COVID, even in younger generations. Interestingly, neurological symptoms after an acute infection are strikingly similar to the symptoms observed after a mild traumatic brain injury (mTBI) or concussion, including dizziness, balance issues, anosmia, and headaches. The possible convergence of biological pathways involved in both will be discussed. The current review is focused on the most commonly described neurological symptoms, as well as the possible molecular mechanisms involved.
Defining a Treatment Model for Self-Management of Fatigue in Rehabilitation of Acquired Brain Injury Using the Rehabilitation Treatment Specification System
Authors: Dornonville de la Cour Frederik Lehman , Norup Anne , Andersen Tonny Elmose , Schow Trine
Published: April 2023

Systematic treatment descriptions to standardize and evaluate management of fatigue after acquired brain injury (ABI) are lacking. The purpose of this multi-phase qualitative study was to formulate a treatment model for promoting self-management of fatigue in rehabilitation of ABI based on practice-based understandings and routines. The study was conducted in a community-based rehabilitation center in Denmark. The model was defined using the Rehabilitation Treatment Specification System. Phase 1 comprised co-production workshops with five service providers (occupational therapists, physiotherapists, and a neuropsychologist) to elicit preliminary treatment theories. In Phase 2, four case studies were conducted on management of fatigue in vocational rehabilitation. Interviews (n = 8) and treatment log entries (n = 76) were analyzed thematically to specify treatment targets and active ingredients. The treatment model comprised five main components: (i) Knowledge and understanding of fatigue, (ii) Interoceptive attention of fatigue, (iii) Acceptance of fatigue, (iv) Activity management, and (v) Self-management of fatigue. For each component, lists of targets and active ingredients are outlined. In conclusion, management of fatigue includes multiple treatment components addressing skills, habits, and mental representations such as knowledge and attitudes. The model articulates treatment theories, which may guide clinical reasoning and facilitate future theory-driven evaluation research.
The Impact of Nordic Walking Pole Length on Gait Kinematic Parameters
Authors: Russo Luca , Belli Guido , Di Blasio Andrea , Lupu Elena , Larion Alin , Fischetti Francesco , Montagnani Eleonora , Di Biase Arrivabene Pierfrancesco , De Angelis Marco
Published: April 2023

Nordic walking (NW) is a popular physical activity used to manage chronic diseases and maintain overall health and fitness status. This study aimed to compare NW to ordinary walking (W) with regard to pole length and to identify kinematic differences associated with different poles’ length (55%, 65% and 75% of the subject’s height, respectively). Twelve male volunteers (21.1 ± 0.7 years; 1.74 ± 0.05 m; 68.9 ± 6.1 kg) were tested in four conditions (W, NW55, NW65 and NW75) at three different speeds (4-5-6 km∗h−1). Each subject performed a total of twelve tests in a random order. Three-dimensional kinematics of upper and lower body were measured for both W and NW, while oxygen consumption levels (VO2) and rating of perceived exertion (RPE) were measured only for NW trials with different poles’ length. NW showed a higher step length, lower elbow motion and higher trunk motion (p < 0.05) compared to W. Additionally, NW65 did not show any kinematic or RPE differences compared to NW55 and NW75. Only NW75 showed a higher elbow joint (p < 0.05) and lower pole (p < 0.05) range of motion compared to NW55 and a higher VO2 (p < 0.05) compared to NW55 and NW65 at 6 km∗h−1. In conclusion, the use of the poles affects the motion of the upper and lower body during gait. Poles with shorter or longer length do not produce particular changes in NW kinematics. However, increasing the length of the pole can be a smart variation in NW to increase exercise metabolic demand without significantly affecting the kinematics and the RPE.
Testing Replicability and Generalizability of the Time on Task Effect
Authors: Krämer Raimund J. , Koch Marco , Levacher Julie , Schmitz Florian
Published: April 2023

The time on task (ToT) effect describes the relationship of the time spent on a cognitive task and the probability of successful task completion. The effect has been shown to vary in size and direction across tests and even within tests, depending on the test taker and item characteristics. Specifically, investing more time has a positive effect on response accuracy for difficult items and low ability test-takers, but a negative effect for easy items and high ability test-takers. The present study sought to test the replicability of this result pattern of the ToT effect across samples independently drawn from the same populations of persons and items. Furthermore, its generalizability was tested in terms of differential correlations across ability tests. To this end, ToT effects were estimated for three different reasoning tests and one test measuring natural sciences knowledge in 10 comparable subsamples with a total N = 2640. Results for the subsamples were highly similar, demonstrating that ToT effects are estimated with sufficient reliability. Generally, faster answers tended to be more accurate, suggesting a relatively effortless processing style. However, with increasing item difficulty and decreasing person ability, the effect flipped to the opposite direction, i.e., higher accuracy with longer processing times. The within-task moderation of the ToT effect can be reconciled with an account on effortful processing or cognitive load. By contrast, the generalizability of the ToT effect across different tests was only moderate. Cross-test relations were stronger in relative terms if performance in the respective tasks was more strongly related. This suggests that individual differences in the ToT effect depend on test characteristics such as their reliabilities but also similarities and differences of their processing requirements.
Influence of Oyster Shell Pyrolysis Temperature on Sediment Permeability and Remediation
Authors: Patil Maheshkumar Prakash , Woo Hee-Eun , Yoon Seokjin , Kim Kyunghoi
Published: April 2023

Permeability is an important aspect of sediment remediation. It is well-known that oyster shells can be used for sediment remediation, however the influence of pyrolysis temperature on sediment permeability remains unknown. In this study, we examined sediment permeability and remediation using crushed oyster shells of less than 5 mm in size that were pyrolyzed at 350 °C (POS350) and 600 °C (POS600) for six hours. Based on the results of the variable head permeability test, POS600 has greater sediment permeability than POS350. In addition, POS600 has greater than POS350 to reduce dissolved inorganic nitrogen (DIN; NH3-N, NO2-N, and NO3-N) and phosphate (PO4-P) from organically enriched sediment because of its higher Ca2+ elution. In conclusion, pyrolysis of oyster shells at 600 °C is more effective than pyrolysis at 350 °C. This finding is true because the transformation of CaCO3 to CaO, which is the source of Ca2+, stimulates pore water flow. Based on these findings, it can be concluded that pyrolyzed oyster shells are beneficial for increasing sediment permeability, thereby helping in the remediation of sediments.
Numerical Analysis of Local Scour of the Offshore Wind Turbines in Taiwan
Authors: Vuong Thi-Hong-Nhi , Wu Tso-Ren , Huang Yi-Xuan , Hsu Tai-Wen
Published: April 2023

Rapid expansions of the offshore wind industry have stimulated a renewed interest in the behavior of offshore wind turbines. Monopile, tripod, and jack-up wind turbines support most offshore wind turbines. These foundations are sensitive to scour, reducing their ultimate capacity and altering their dynamic response. However, the existing approaches ignore the seabed’s rheological properties in the scour process. This study focuses on the scour development around the wind turbine foundation in the Changhua wind farm in Taiwan. The simulation results explain the influence of different hydrodynamic mechanisms on the local scours in a cohesive fluid, such as regular waves, random waves, and constant currents. A newly non-Newtonian fluid model, the Discontinuous Bi-viscous Model (DBM), reproduces closet mud material nature without many empirical coefficients and an empirical formula. This new rheology model is integrated and coupled into the Splash3D model, which resolves the Navier–Stokes equations with a PLIC-VOF surface-tracking algorithm. The deformation of the scour hole, the backfilling, and the maximum scour depth are exhibited around the wind turbines. Waves, including regular and irregular waves, do not increase the scour depth compared with currents only. In the case of random wave–current coupling, the results present a signal of scour evolution. However, the scour depth is shallow at 0.033≤S/D≤0.046.
A Review on Optimal Design of Fluid Machinery Using Machine Learning Techniques
Authors: Xu Bin , Deng Jiali , Liu Xingyu , Chang Ailian , Chen Jiuyu , Zhang Desheng
Published: April 2023

The design of fluid machinery is a complex task that requires careful consideration of various factors that are interdependent. The correlation between performance parameters and geometric parameters is highly intricate and sensitive, displaying strong nonlinear characteristics. Machine learning techniques have proven to be effective in assisting with optimal fluid machinery design. However, there is a scarcity of literature on this subject. This study aims to present a state-of-the-art review on the optimal design of fluid machinery using machine learning techniques. Machine learning applications primarily involve constructing surrogate models or reduced-order models to explore the correlation between design variables or the relationship between design variables and performance. This paper provides a comprehensive summary of the research status of fluid machinery optimization design, machine learning methods, and the current application of machine learning in fluid machinery optimization design. Additionally, it offers insights into future research directions and recommendations for machine learning techniques in optimal fluid machinery design.
Hydrokinetic Power Potential in Spanish Coasts Using a Novel Turbine Design
Authors: Ibrahim Mahmoud I. , Legaz María José
Published: April 2023

Nowadays, there is great concern about obtaining clean energy. Governments around the world are boosting renewable energy resources. Oceans provide abundant renewable energy resources, including tidal, wave, and current energy. It seems that ocean currents are one of the most promising ways to obtain energy from the oceans. The goal of this paper is to assess the hydrokinetic power potential in three different areas of the Spanish coast using a novel turbine design, named the fin-ring turbine. The patented turbine was previously power tested in 2014 in the Gulf of Mexico and numerically validated in the literature. A three-dimensional computational fluid dynamics (CFD) simulation of the novel current turbine is presented, including mesh sensitivity and turbulence studies. The turbine’s performance represented in TSR-Cp is discussed. The turbine was simulated in different regions with several current speeds, focusing on the Spanish coast. The results are very promising, with upper limit power coefficients of 37.5%, and 36.5% as a lower limit. Also, the comparisons with power test data available in the literature show very satisfactory agreement. The results highlight the superiority of the turbine in lower currents and present the suitability of the turbine’s applicability.
Review of Endometrial Receptivity Array: A Personalized Approach to Embryo Transfer and Its Clinical Applications
Authors: Rubin Sarah C. , Abdulkadir Mawerdi , Lewis Joshua , Harutyunyan Aleksandr , Hirani Rahim , Grimes Cara L.
Published: April 2023

Successful outcomes of in vitro fertilization (IVF) rely on both the formation of a chromosomally normal embryo and its implantation in a receptive endometrium. Pre-implantation genetic testing for aneuploidy (PGT-A) has been widely accepted as a tool to assess the viability of an embryo. In 2011, the endometrial receptivity array (ERA) was first published as a tool to determine when the endometrium is most receptive to an embryo, commonly referred to as the “window of implantation” (WOI). The ERA uses molecular arrays to assess proliferation and differentiation in the endometrium and screens for inflammatory markers. Unlike PGT-A, there has been dissent within the field concerning the efficacy of the ERA. Many studies that contest the success of the ERA found that it did not improve pregnancy outcomes in patients with an already-good prognosis. Alternatively, studies that utilized the ERA in patients with repeated implantation failure (RIF) and transfer of known euploid embryos demonstrated improved outcomes. This review aims to describe the ERA as a novel technique, review the various settings that the ERA may be used in, such as natural frozen embryo transfer (nFET) and hormone replacement therapy frozen embryo transfer (HRT-FET), and provide a summary of the recent clinical data for embryo transfers in patients with RIF utilizing the ERA.
Influence of Clinical Factors on the Quality of Life in Romanian People with Epilepsy—A Follow-Up Study in Real-Life Clinical Practice
Authors: Cioriceanu Ionut-Horia , Constantin Dan-Alexandru , Bobescu Elena , Marceanu Luigi Geo , Rogozea Liliana
Published: April 2023

Background: This study aimed to assess the influence of various clinical factors on the quality of life perception of patients with epilepsy over a follow-up period in current clinical practice. Methods: Thirty-five PWE evaluated via video-electro-encephalography in the Clinical Hospital of Psychiatry and Neurology in Brasov, Romania, were included, and the quality of life was assessed using the Romanian version of the QOLIE-31-P questionnaire. Results: At baseline, the mean age was 40.03 (±14.63) years; the mean duration of epilepsy was 11.46 (±12.90) years; the mean age at the first seizure was 28.57 (±18.72); and the mean duration between evaluations was 23.46 (±7.54) months. The mean (SD) QOLIE-31-P total score at the initial visit (68.54 ±15.89) was lower than the mean (SD) QOLIE-31-P total score at the follow-up (74.15 ± 17.09). Patients with epileptiform activity recorded via video-electro-encephalography, using polytherapy, those with uncontrolled seizures, and those with one or more seizures per month had statistically significantly lower QOLIE-31-P total scores at baseline and follow-up. Multiple linear regression analyses revealed seizure frequency as a significant inverse predictor of quality of life in both evaluations. Conclusions: The QOLIE-31-P total score was improved during the follow-up period, and medical professionals should use instruments to evaluate quality of life and identify patterns while trying to improve the outcomes of patients with epilepsy.
Patients with Non-Alcoholic Fatty Liver Disease and Alcohol Dehydrogenase 1B/Aldehyde Dehydrogenase 2 Mutant Gene Have Higher Values of Serum Alanine Transaminase
Authors: Chien Tsuo-Hsuan , Lin Chih-Lang , Chen Li-Wei , Chien Cheng-Hung , Hu Ching-Chih
Published: April 2023

Patients with non-alcoholic fatty liver disease (NAFLD) share similar pathophysiologies to those of patients with alcohol liver disease. Alcoholic metabolic enzyme-related genes (alcohol dehydrogenase 1B (ADH1B) and aldehyde dehydrogenase 2 (ALDH2)) may be associated with pathophysiology in NAFLD patients. In this study, the association between ADH1B/ALDH2 gene polymorphism and serum metabolic factors, body statures, and hepatic steatosis/fibrosis status was evaluated in patients with NAFLD. Using biochemistry data, abdominal ultrasonography, fibrosis evaluation (Kpa), and steatosis evaluation (CAP), ADH1B gene SNP rs1229984 and ALDH2 gene SNP rs671 polymorphism were analyzed in sixty-six patients from 1 January 2022 to 31 December 2022. The percentage of the mutant type (GA + AA) was 87.9% (58/66) in the ADH1B allele and 45.5% (30/66) in the ALDH2 allele. Patients with the mutant-type ADH1B/ALDH2 allele had higher values of alanine aminotransferase (ALT) than the wild type (β = 0.273, p = 0.04). No association was observed between body mass index, serum metabolic factors (sugar and lipid profile), CAP, kPa, and ADH1B/ALDH2. A high proportion of the mutant-type ADH1B allele (87.9%) and ALDH2 allele (45.5%) was observed in patients with NAFLD. No association was observed between ADH1B/ALDH2 allele, BMI, and hepatic steatosis/fibrosis. Patients with the mutant-type ADH1B/ALDH2 allele had higher values of ALT than those with the wild type.
The Effect of ECB Unconventional Monetary Policy on Firms’ Performance during the Global Financial Crisis
Authors: Basdekis Charalampos , Christopoulos Apostolos , Gakias Evgenios , Katsampoxakis Ioannis
Published: April 2023

This study aims to analyse and investigate the most important factors affecting the performance of listed firms in the Athens Stock Exchange, emphasising capital structure, size and sovereign debt rate as a proxy for firms’ borrowing rate. Yet, the most remarkable factor taken into consideration to affect firms’ profitability is the delta of ECB assets as a proxy of the ECB’s strategy during the financial crisis. Indeed, the examination of the ECB’s delta is innovative for such analysis and differentiates this study from previous ones. The survey was conducted for the period 2005–2019, and the sample consisted of 49 firms from all sectors of the economic activity, except for the financial sector, as its companies’ capital structure is subject to supervisory restrictions. Thus, the financial sector’s inclusion in the sample would affect its homogeneity. The sample is divided into two sub periods, based on the statement of ECB’s president Mario Draghi “Whatever it takes,” in 2012, expressing the ECB’s strategy for backing and boosting the Eurozone economy. The empirical approach of our analysis is based on a panel data analysis, which allows the combination of both cross-section and time series data. In addition, we develop, test and analyse four specifications of our main model, each one with a different dependent variable as a proxy for profitability. These variables are EPS (earnings per share), ROE (return on equity), ROA (return on assets) and TOBIN’s Q. Our findings lead to some very interesting conclusions, which in most cases are consistent for the specification of all the examined models. More specifically, the results show a negative influence of debt-to-equity ratio and 10-year Greek yield bond on firms’ profitability regardless of the proxy used (EPS, ROE or TOBIN’s Q), while there is a positive impact of firms’ size and the delta of ECB’s total assets on firms’ profitability. However, the soundest outcome of this study shows that the expansion of the ECB’s balance sheet and the unconventional policy does contribute to the improvement of firms’ performance and economic stability. The findings become even more impressive, considering the turning of ECB’s strategy after the implementation of the unconventional policy in 2012. Our findings are useful for policymakers of international institutions and government authorities as we propose strategies favouring economic stability and economic activity but also for managers and stakeholders who can identify the factors which determine firms’ performance in order to apply the best policies for financing, investments and growth.