bluuebunny/crossref_metadata_2025 huggingface dataset:
Harnessing the Heart’s Magnetic Field for Advanced Diagnostic Techniques
Authors: Elfouly Tarek , Alouani Ali
Published: September 2024

Heart diseases remain one of the leading causes of morbidity and mortality worldwide, necessitating innovative diagnostic methods for early detection and intervention. An electrocardiogram (ECG) is a well-known technique for the preliminary diagnosis of heart conditions. However, it can not be used for continuous monitoring due to skin irritation. It is well known that every body organ generates a magnetic field, and the heart generates peak amplitudes of about 10 to 100 pT (measured at a distance of about 3 cm above the chest). This poses challenges to capturing such signals. This paper reviews the different techniques used to capture the heart’s magnetic signals along with their limitations. In addition, this paper provides a comprehensive review of the different approaches that use the heart-generated magnetic field to diagnose several heart diseases. This research reveals two aspects. First, as a noninvasive tool, the use of the heart’s magnetic field signal can lead to more sensitive advanced heart disease diagnosis tools, especially when continuous monitoring is possible and affordable. Second, its current use is limited due to the lack of accurate, affordable, and portable sensing technology.
Development and Test of Low-Cost Multi-Channel Multi-Frequency Lock-In Amplifier for Health and Environment Sensing
Authors: Pollastrone Fabio , Fiorani Luca , Bisauriya Ramanand , Menicucci Ivano , Ciceroni Claudio , Pizzoferrato Roberto
Published: September 2024

Optical-based sensing techniques and instruments, such as fluorometric systems, absorbance-based sensors, and photoacoustic spectrometers, are important tools for detecting food fraud, adulteration, and contamination for health and environmental purposes. All the aforementioned optical equipments generally require one or more low-frequency Lock-In Amplifiers (LIAs) to extract the signal of interest from background noise. In the cited applications, the required LIA frequency is quite low (up to 1 kHz), and this leads to a simplification of the hardware with consequent good results in portability, reduced size, weight, and low-cost characteristics. The present system, called ENEA DSP Box Due, is based on a very inexpensive microcontroller proto-board and can replace four commercial LIAs, resulting in significant savings in both cost and space. Furthermore, it incorporates a dual-channel oscilloscope and a sinusoidal function generator. This article outlines the architecture of the ENEA DSP Box Due, its electrical characterization, and its applications within a project concerning laser techniques for food and water safety.
Research on Multi-Core Curvature Sensing Measurement Based on PPP-BOTDA
Authors: Liu Zijuan , Li Yongqian , Zhang Lixin , Wang Lei
Published: September 2024

To address the issue of spatial resolution limitations in traditional Brillouin optical time-domain analysis systems due to phonon lifetime constraints, we employed pre-pumped pulse technology. Additionally, to mitigate the double-peak phenomenon observed in pre-pumped Brillouin optical time-domain analysis systems, we implemented a two-sided band interference method to reduce the linewidth of the double-peak fitting. We conducted bending measurements on three eccentric cores and intermediate cores spaced 120° apart. Our results demonstrate that the system described in this paper can achieve a spatial resolution of 30 cm, with bimodal linewidths of 23.1 MHz and 16.0 MHz. Using the parallel transmission frame algorithm, we determined the curvature of a seven-core fiber with a curvature diameter of approximately 10 cm to be 20.67 m−1, with an error margin of 3.2%.
Bioelectrical Impedance Vector Analysis (BIVA) for Assessment of Hydration Status: A Comparison between Endurance and Strength University Athletes
Authors: Abdelnour Maria , Berkachy Rédina , Nasreddine Lara , Fares Elie-Jacques
Published: September 2024

Introduction: Athletic performance is greatly impacted by hydration status. The combination of several techniques is recommended to accurately measure water losses and gains. Aim: The aim of this study is to assess the validity of bioelectrical impedance vector analysis (BIVA) as a tool for measuring hydration status in endurance and strength athletes. Methods: A total of 148 athletes were evaluated on one experimental day, pre- and post-training. Urine samples were collected and analyzed for color and specific gravity. Body weight changes were measured, sweat rate was calculated, and BIVA was performed. Reference ellipses were plotted using data of 200 healthy non-athletic individuals. Results: A moderate significant agreement was noted between raw bioelectrical values and urine specific gravity (USG) (p > 0.05). The sensitivity of classic BIVA in detecting minor changes in hydration status is confirmed both graphically and statistically. R/h and Z statistically significantly decreased post-training. Male athletes exhibited a specific BIA vector distribution compared to the reference population and were slightly more hydrated than female athletes. Conclusions: BIVA validation may be an essential step to allow its use among university students to assess dehydration in a non-invasive, practical, and inexpensive way.
A Joint Active Damping Strategy Based on LCL-Type Grid-Connected Inverters for Grid Current Feedback and PCC Voltage Unit Feedforward
Authors: Ke Shanwen , Liang Bo
Published: September 2024

The negative high-pass filter feedback of the grid current (NFGCF) can offer active damping for the LCL-type grid-connected inverter. Due to the control delay in digital control systems, this damping can cause the system to exhibit non-minimum phase behavior within specific frequency ranges. This study proposes a joint active damping approach that combines grid current feedback and the point of common coupling (PCC) voltage unit feedforward. The proposed method introduces a dynamic damping region that varies with grid impedance. By developing suitable damping loop control parameters, this region can span the entire frequency range, even exceeding the Nyquist frequency fs/2. The research results demonstrate that the proposed approach enhances robustness against variations in grid impedance and eliminates non-minimum phase behavior. Simulation and experimental outcomes validate the effectiveness of this joint active damping method.
The Optimization of the Osborne Extraction Method for the Fractionation and Characterization of Oat Proteins
Authors: Sagu Sorel Tchewonpi , Wiedemann Lara Talea , Nichani Kapil , Henze Andrea , Rawel Harshadrai M.
Published: September 2024

The growing number of wheat-related allergies worldwide has resulted in a new trend towards gluten-free alternatives. In this context, alternative cereals such as sorghum and oats are attracting new interest. Given the limited data available, the question of whether these cereals are completely safe and gluten-free for allergy sufferers remains open. One of the key steps in protein research is their efficient extraction. In this work, the Osborne sequential extraction method was developed and optimized using the response surface methodology in order to fractionate oat proteins. An optimized desirability of 0.986 was achieved with an extraction time of 4.7 min, a speed of 6, and a sample/solvent ratio of 5. The corresponding optimized responses were 8.7, 4.0, and 5.1% for the extraction yields of the avenin, avenalin, and albumin/globulin fractions, respectively. Further characterization of the extracts was carried out on 24 homogeneous and commercial oat samples via LC-MS/MS, targeting six potentially allergenic proteins. The avenin-E protein featured prominently, with relative contents of 60.7, 32.2, 58.0, and 59.8% in the total extract, avenin, avenalin, and albumin/globulin fractions, respectively, while the Avenin-3, ATI-2, avenin, SSG2, and SSG1 proteins in the total extract showed levels of 16.4, 9.3, 6.6, 4.8, and 2.2%, respectively. The preliminary results of an ELISA performed on the different fractions revealed low levels of gluten (from 1.24 ± 0.14 to 3.61 ± 0.16 mg/kg), which were well below the threshold limit of 20 mg/kg. These results support the hypothesis that oats can be a safe food for people suffering from cereal-related allergies. These results open the door to further studies into the comprehensive characterization of oat proteins.
The Central Functions of Cities in Poland in Light of Administrative Reform
Authors: Przybyła Katarzyna , Hełdak Maria , Kachniarz Marian , Ramsey David , Kulczyk-Dynowska Alina , Szara Katarzyna
Published: September 2024

The aim of this article is to determine and assess the changes in the central functions of major Polish cities in the context of Poland’s 1999 administrative reform. This study covers a period of twenty-two years (1998–2019), which provides a solid perspective for evaluating the functional changes occurring within the current territorial division of the country. The conclusions indicate that the dynamics of changes in central functions in former provincial capitals differed from those in cities that retained this status. At the same time, it was found that the administrative reform sanctioned the scope and scale of earlier socio-economic dependencies in the present regional centers. The research results suggest that administrative reforms in Poland solidified existing socio-economic development paths in cities, but were not the sole catalyst for change. They point to a complex interaction of factors influencing urban evolution. By utilizing indicators that measure the centrality of cities in relation to their demographic potential, this research offers a quantitative assessment of the level of development of central functions, which is crucial for monitoring progress towards sustainable urbanization. By providing this information, this research also contributes to a broader discussion on the advancement and dynamics of sustainable urban development.
Travel Time Variability in Urban Mobility: Exploring Transportation System Reliability Performance Using Ridesharing Data
Authors: Sun Yuxin , Chen Ying
Published: September 2024

Travel time variability (TTV) is a crucial indicator of transportation network performance, assessing travel time reliability and delays. This study investigates TTV metrics within the context of shared mobility using probe data from transportation network companies (TNCs) in Chicago, Los Angeles, and Dallas–Fort Worth. Eight reliability metrics are analyzed and compared for each origin–destination (OD) pair in the network, including standard deviation (SD), the Planning Time Index (PTI), the Travel Time Index (TTI), the Buffer Index (BI), On-time Measures PR (alpha), and the Misery Index (MI), to evaluate their effectiveness in clustering OD pairs using K-means clustering. The findings confirm that SD, PTI, and MI are particularly effective in measuring travel time reliability and clustering within urban systems. This study identifies the most unbalanced supply–demand OD pairs/regions in each city, noting that low/medium-SD clusters around metropolitan airports indicate stable travel times even in high-demand zones, while high-SD clusters in downtown areas reveal significant traffic demands and unreliability. These patterns become more pronounced in study areas with multiple city centers. This study highlights the need for targeted strategies to enhance travel time reliability, particularly in regions like Dallas–Fort Worth, where public transportation alternatives are limited.
Effectiveness of Cool and Green Roofs Inside and Outside Buildings in the Brazilian Context
Authors: Scolaro Taylana Piccinini , Ghisi Enedir , Silva Cristina Matos
Published: September 2024

Several studies have assessed the thermal performance of green and cool roofs. However, few have comprehensively addressed Brazilian buildings and climates, considering indoor and outdoor environments. Considering three Brazilian cities, this study aims to assess the performance of green and cool roofs compared with traditional fibre cement roofs in a typical multifamily residential building. Energy consumption, thermal comfort, and outside surface temperature were assessed using computer simulation. The results show that the cool roofs performed better in cities with warmer climates (e.g., Cfa and Aw), reducing electricity consumption by up to 24.8% compared with traditional roofs. Green roofs are better suited for colder climates (e.g., Cfb), with up to 28.2% energy savings. Green roofs provided the highest percentage of thermal comfort hours in all climates. Cool and green roofs provided hourly reductions in outside roof surface temperature of up to 16.5 °C and 28.4 °C, respectively, compared with the traditional roof. This work reinforces that the choice between these two roof types for each city depends on the parameter used for comparison. Based on the relevant information applied to Brazilian buildings and representative climates presented, this work provided recommendations for urban planning policies and building regulations in Brazil.
Envisioning the Future of Mobility: A Well-Being-Oriented Approach
Authors: Elsamani Yousif , Kajikawa Yuya
Published: September 2024

Mobility, a vital part of daily life, significantly impacts human well-being. Understanding this relationship is crucial for shaping the future trajectory of mobility, a connection often overlooked in previous research. This study explores the complex relationship between mobility and well-being and proposes a holistic framework for mobility’s future, prioritizing individual and societal well-being. The motivation for this research stems from the growing need to balance technological advancements in transportation with the well-being of diverse populations, especially as the mobility landscape evolves with innovations like autonomous vehicles and intelligent mobility solutions. We employ bibliometric methods, analyzing 53,588 academic articles to identify key themes and research trends related to mobility and well-being. This study categorizes these articles into thematic clusters using the Louvain modularity maximization algorithm, which facilitates the formation of cohesive groups based on citation patterns. Our findings underline the significant impact of mobility on physical, mental, psychological, financial, and social well-being. The proposed framework features four pillars: vehicle, infrastructure and environment, mobility stakeholders, and policy. This framework underscores the importance of collaboration between institutional and individual actions in shaping a future mobility landscape that is technologically advanced, socially responsible, and conducive to an improved quality of life.
The Practical Impact of Price-Based Demand-Side Management for Occupants of an Office Building Connected to a Renewable Energy Microgrid
Authors: Asaleye Damilola A. , Murphy Darren J. , Shine Philip , Murphy Michael D.
Published: September 2024

This paper examined the practical impact of price-based demand-side management (DSM) for occupants of an office building connected to a renewable energy microgrid. There has been an absence of studies that have analyzed occupant reactions, in terms of perceived practicality, regarding the implementation of DSM in conjunction with factors including renewable energy generation, load shifting and energy costs. An increased understanding of the practicality of DSM will support future improvements in building energy efficiency and sustainability. Ten occupants of the National Build Energy Retrofit Test-bed (NBERT) were selected as a case study. The electricity consumption pattern of the NBERT occupants was derived over a period of two years. Five unique DSM schedules were developed for each of the NBERT occupants, and a survey was carried out to investigate the practicality of these DSM schedules. A real-time electricity pricing tariff, electricity CO2 intensity, three feed-in tariffs and two microgrid control methods were evaluated to assess the practicality of each DSM schedule on the ten NBERT occupants. The results showed that the incorporation of onsite renewable energy generation with price-based DSM had a positive impact (r = 0.69–0.75) on occupant practicality. Onsite renewable energy generation was able to offset the demand for expensive electricity from the grid during peak hours, which aligned with the occupants’ typical work schedules. However, the introduction of a feed-in tariff with a renewable energy microgrid made price-based DSM less practical (r = 0.15–0.64).
Estimation of Urban High-Quality Development Level Using a Three-Stage Stacks-Based Measure Model: A Case Study of Urban Agglomerations in the Yellow River Basin
Authors: Liu Sisi , Yang Suchang , Liu Ningyi
Published: September 2024

The high-quality development paradigm, which emphasizes the organic unity of efficiency, equity, and sustainability, has gained increasing global recognition as an extension of the concept of sustainable green development. In this study, we use green development efficiency as a metric of high-quality development and employ a three-stage Stacks-based Measure Model (SBM) in order to assess the true green development efficiency (GDE) levels of urban agglomerations in China’s Yellow River Basin (YRB) from 2011 to 2020. The results indicate that external environmental factors significantly impacted the green development efficiency levels of these urban agglomerations; after removing these factors, their green development efficiency shifted from trendless fluctuations to more consistent upward trends. Additionally, the disparities between different urban agglomerations are the primary sources of overall differences in green development efficiency in the YRB. Influenced by economic development levels and administrative divisions, the degree of internal development imbalance varies among urban agglomerations; however, regional disparities show a decreasing trend, indicating a catch-up effect. Based on these findings, we further propose relevant policy recommendations in this paper. The results of this study help us to understand the current status and trends of high-quality development in the urban agglomerations of the YRB, providing empirical evidence for policy formulation.
Entrepreneurial Leadership and Green Innovative Work Behavior: The Role of Green Soft and Hard Talent Management with a Dual Theoretical Lens
Authors: Yan Li , Ahmed Zeeshan , Khosa Mishal , Fahmi Omar Faqera Abdulaziz , Kayode Ibikunle Afeez , Rashid Khan Ayesha
Published: September 2024

The implementation of green initiatives by hospitality employees can significantly enhance the environmental sustainability of hospitality firms. However, there is a lack of research exploring the mechanisms influencing hospitality employees’ green innovative work behavior (GIWB). Through the mediation paths of green soft talent management (GSTM) and green hard talent management (GHTM), this study seeks to investigate how entrepreneurial leadership promotes GIWB. The data from 366 employees and managers in Pakistan’s hospitality industry were collected through a survey method using partial least squares structural equation modeling (PLS-SEM). The findings reveal that the effects of entrepreneurial leadership on GIWB, the direct effects of GSTM and GHTM, and the interaction effects between entrepreneurial leadership and GIWB are substantial. Based on the upper echelons theory and the resource-based view concept, this study extends research on the leadership–GIWB macro nexus and increases our understanding of the mechanisms behind employee GIWB, particularly in the hospitality setting.
A Study on the Mechanisms of Coal Fly Ash to Improve the CO2 Capture Efficiency of Calcium-Based Adsorbents
Authors: Zhao Ziyu , Zhang Kefan , Luo Jianfeng , Wu Meixuan , Wang Xiyue , Wang Keke , Liu Shengyu
Published: September 2024

Utilizing calcium-based adsorbents for CO2 adsorption through cyclic calcination/carbonization is one of the most cost-effective methods for carbon emission reduction. In order to improve the cycle stability of the adsorbents and the capture efficiency of CO2, this study used industrial solid waste coal fly ash for the hydration treatment of calcium-based adsorbent to explore the variations in the cyclic adsorption performance of the adsorbent under different doping ratios and hydration conditions. By means of various characterization techniques, the microscopic mechanism for improving the performance of the modified adsorbent was analyzed from the perspectives of chemical composition, physical structure, and surface functional groups of the adsorbents. The results demonstrated that the modification of coal fly ash could significantly enhance the carbonation performance and cycle stability of the adsorbent in multiple CO2 capture processes. The modified material doped with 5% coal fly ash had the highest total CO2 adsorption capacity, which increased by 13.7% compared to before modification. Additionally, the modified material doped with 10% coal fly ash exhibited the strongest cyclic adsorption capacity, which was 14.0% higher than that before modification, and the adsorption attenuation rate decreased by 32.2%. The characterization results showed that the reaction between calcium oxide and coal fly ash formed CaSiO3 and Ca12Al14O33 during the modification process, which was the primary reason for the improvement in the CO2 capture performance of the modified materials. This study provided a new perspective on the resource utilization of solid waste fly ash and efficient CO2 capture.
Integration of Cybersecurity, Usability, and Human-Computer Interaction for Securing Energy Management Systems
Authors: Albarrak Abdullah M.
Published: September 2024

The energy sector is a critical contributor to the growth and development of any country’s economy. However, ensuring robust cybersecurity within the context of smart energy services presents persistent usability challenges in an increasingly digital environment. This study explores the intersection of human-computer interaction (HCI), cybersecurity, and usability to identify and address issues that impact the overall security of smart energy management systems. By analyzing the complex relationships between users and security protocols, this research aims to enhance the security framework, promote better user adherence, and improve system usability. The study focuses on three primary objectives: (1) identifying the most prevalent usability issues in current cybersecurity practices; (2) examining the relationship between HCI and user compliance with security measures; and (3) proposing strategies to improve cybersecurity usability by leveraging HCI principles. Hybrid approaches utilizing artificial intelligence facilitate empirical analysis and framework evaluation. Additionally, a comparative study with six existing models has been conducted. By envisioning a future where security measures not only ensure enhanced protection but also integrate seamlessly into user experiences, this approach seeks to provide valuable insights into ongoing cybersecurity discussions and contribute to a more resilient security landscape against evolving digital threats.
Land Use Simulation and Ecological Network Construction around Poyang Lake Area in China under the Goal of Sustainable Development
Authors: Luo Zhijun , Yang Xiaofang , Luo Songkai
Published: September 2024

The pivotal aspects of enhancing regional ecosystem services and augmenting socioeconomic growth lie in optimizing the land-space development and protection strategies, coupled with the establishment of a robust ecological network (EN). This article examines the Poyang Lake area and employs the MOP model, NSGA-II, and PLUS model to determine the best sustainable land use strategy. Subsequently, the MSPA, InVEST model, circuit theory, complex network, and others are employed to construct and analyze the land-space EN across three time periods. Ultimately, the EN is optimized based on spatial protection priority, ecological obstacle areas, and ecological nodes. The results show the following: (1) From 2005 to 2035, more construction land will be developed around the Greater Nanchang area and other urban centers. In the BAU scenario, construction land will expand faster, while cultivated land, forest, grassland, and bare land will continue to decline. In the SD scenario, the alteration to comparable land is minimal, the growth rate of construction land will slow, cultivated land, forest, grassland, and bare land will all decline little, and the water area will increase slightly; (2) While the area of ecological sources is decreased and ecological corridors become longer and narrower in the BAU scenario, the spatial distribution of ENs in different periods is small, and the quantitative structure and spatial distribution of ecological sources and corridors are essentially unchanged in the SD scenario; (3) Based on the topological structure of ENs, it is found that the clustering of nodes in the SD scenario is more obvious, the importance of ecological sources is enhanced, the efficiency of information transmission is improved, and the radiation range is wider and more stable; (4) The greatest priority ecological sources in each period are concentrated around Poyang Lake. In the SD scenario, the priority of ecological sources improves, and 7025 km2 of ecological obstacle restoration area is identified, with 41, 31, and 36 ecological breakpoints in the first, second, and third levels. The study’s findings can assist and shape theoretical and practical approaches to land governance and sustainable development in great lake areas.
Supramolecular Chemistry of Polymer-Based Molecular Tweezers: A Minireview
Authors: Vafakish Bahareh , Wilson Lee D.
Published: September 2024

Polymer-based molecular tweezers have emerged as a prominent research area due to their enhanced ability to form host–guest complexes, driven by advancements in their design and synthesis. The impact of the spacer structure on the tweezers is predominant. They can be rigid, flexible, and stimuli-responsive. Herein, a new generation of molecular tweezers is introduced as polymer-based molecular tweezers. The integration of molecular tweezers onto biopolymers has significantly expanded their potential applications, making them promising candidates, especially in drug delivery, owing to their biocompatibility, adaptive structural features, and versatile interaction capabilities. The unique structure of polymer-based molecular tweezers, particularly when integrated with biopolymers, creates a unique nano-environment that enhances their interaction with guest molecules. This minireview focuses on the synthesis and applications of polymer-based molecular tweezers and examines how the incorporation of various spacers affects their binding affinity and specificity. These features highlight the advancement of these polymer-based systems, emphasizing their potential applications, particularly in drug delivery, water treatment technology, and future research opportunities.
Kelvin Probe Force Microscopy, Current Mapping, and Optical Properties of Hybrid ZnO Nanorods/Ag Nanoparticles
Authors: Musa Ishaq
Published: September 2024

The optical characteristics and electrical behavior of zinc oxide nanorods (ZnO-NRs) and silver nanoparticles (Ag-NPs) were investigated using advanced scanning probe microscopy techniques. The study revealed that the ZnO nanorods had a length of about 350 nm, while the Ag nanoparticles were spherical with heights ranging from 5 to 14 nm. Measurements with Kelvin probe force microscopy (KPFM) showed that the work functions of ZnO nanorods were approximately 4.55 eV, higher than that of bulk ZnO, and the work function of Ag nanoparticles ranged from 4.54 to 4.56 eV. The electrical characterization of ZnO nanorods, silver nanoparticles, and their hybrid was also conducted using conductive atomic force microscopy (C-AFM) to determine the local current-voltage (I-V) characteristics, which revealed a characteristic similar to that of a Schottky diode. The current-voltage characteristic curves of ZnO nanorods and Ag nanoparticles both showed an increase in current at around 1 V, and the hybrid ZnONRs/AgNP exhibited an increase in turn-on voltage at around 2.5 volts. This suggested that the presence of Ag nanoparticles enhanced the electrical properties of ZnO nanorods, improving the charge carrier mobility and conduction mechanisms through a Schottky junction. The investigation also explored the optical properties of ZnO-NRs, AgNPs, and their hybrid, revealing absorption bands at 3.11 eV and 3.18 eV for ZnO-NRs and AgNPs, respectively. The hybrid material showed absorption at 3.13 eV, indicating enhanced absorption, and the presence of AgNP affected the optical properties of ZnO-NR, resulting in increased photoluminescence intensity and slightly changes in peak positions.
Indolent Adenocarcinoma In Situ Mistaken as Aggressive during Lung Cancer Screening Program: To Heal or to Harm?
Authors: Orlandi Riccardo , Mattioni Giovanni , Rolli Luigi , Pastorino Ugo
Published: September 2024

Background: Low-dose computed tomography-based lung cancer screening (LCS) has demonstrated efficacy in reducing lung cancer mortality. However, concerns about overdiagnosis and overtreatment hinder global LCS implementation. Methods: Here, we report the unique case of a slow-growing 1 cm pure ground-glass opacity (GGO) of the lung, known for 15 years, which unexpectedly developed into a 5 cm mixed GGO within 1 year, with an increased solid component and FDG-PET uptake. Results: The patient, asymptomatic, underwent right upper lobectomy and lymphadenectomy, even complicated with postoperative chylothorax, later revealing to be affected by only an unchanged adenocarcinoma in situ (AIS). Conclusions: This case serves as a reminder of the potential behavior of pre-invasive lesions, which can mimic invasive neoplasia and may lead to overtreatment, and underscores the challenge of distinguishing indolent lesions from potentially aggressive malignancies in LCS, highlighting the need for the ongoing refinement of LCS protocols to mitigate this risk.
Experiments of Ultrasonic Positioning System with Symmetrical Array Used in Jiangmen Underground Neutrino Observatory
Authors: Zhu Guolei , Yang Wenxin , Teng Duo , Wang Qi , Hui Jiaqi , Lian Jie
Published: September 2024

The experimental environment of the JUNO is a spherical container filled with a liquid scintillator (alkylbenzene) with a diameter of about 35 m. To observe neutrino interactions in alkylbenzene with photomultiplier tubes (PMTs) and to precisely measure the neutrino properties in this environment, it is necessary to design a high-precision localization system for the PMT device. In this paper, we report the design of an ultrasonic localization system with a symmetrical receiving array, based on the construction of an experimental setup that reproduces the configuration of JUNO’s environment. We show through positioning consistency and accuracy measurements that the ultrasonic localization system has a high localization accuracy and can perform effective localization in an alkylbenzene solution with 98% purity.
The Contrasting Effects of Bothrops lanceolatus and Bothrops atrox Venom on Procoagulant Activity and Thrombus Stability under Blood Flow Conditions
Authors: Radouani Fatima , Jalta Prisca , Rapon Caroline , Lezin Chloe , Branford Chelsea , Florentin Jonathan , Gutierrez Jose Maria , Resiere Dabor , Neviere Remi , Pierre-Louis Olivier
Published: September 2024

Background: Consumption coagulopathy and hemorrhagic syndrome are the typical features of Bothrops sp. snake envenoming. In contrast, B. lanceolatus envenoming can induce thrombotic complications. Our aim was to test whether crude B. lanceolatus and B. atrox venoms would display procoagulant activity and induce thrombus formation under flow conditions. Methods and Principal Findings: Fibrin formation in human plasma was observed for B. lanceolatus venom at 250–1000 ng/mL concentrations, which also induced clot formation in purified human fibrinogen, indicating thrombin-like activity. The degradation of fibrinogen confirmed the fibrinogenolytic activity of B. lanceolatus venom. B. lanceolatus venom displayed consistent thrombin-like and kallikrein-like activity increases in plasma conditions. The well-known procoagulant B. atrox venom activated plasmatic coagulation factors in vitro and induced firm thrombus formation under high shear rate conditions. In contrast, B. lanceolatus venom induced the formation of fragile thrombi that could not resist shear stress. Conclusions: Our results suggest that crude B. lanceolatus venom displays amidolytic activity and can activate the coagulation cascade, leading to prothrombin activation. B. lanceolatus venom induces the formation of an unstable thrombus under flow conditions, which can be prevented by the specific monovalent antivenom Bothrofav®.
Astaxanthin Alleviates Hepatic Lipid Metabolic Dysregulation Induced by Microcystin-LR
Authors: Tan Qinmei , Chu Hanyu , Wei Jia , Yan Sisi , Sun Xiaoya , Wang Jiangping , Zhu Lemei , Yang Fei
Published: September 2024

Microcystin-LR (MC-LR), frequently generated by cyanobacteria, has been demonstrated to raise the likelihood of liver disease. Few previous studies have explored the potential antagonist against MC-LR. Astaxanthin (ASX) has been shown to possess various beneficial effects in regulating lipid metabolism in the liver. However, whether ASX could alleviate MC-LR-induced hepatic lipid metabolic dysregulation is as yet unclear. In this work, the important roles and mechanisms of ASX in countering MC-LR-induced liver damage and lipid metabolic dysregulation were explored for the first time. The findings revealed that ASX not only prevented weight loss but also enhanced liver health after MC-LR exposure. Moreover, ASX effectively decreased triglyceride, total cholesterol, aspartate transaminase, and alanine aminotransferase contents in mice that were elevated by MC-LR. Histological observation showed that ASX significantly alleviated lipid accumulation and inflammation induced by MC-LR. Mechanically, ASX could significantly diminish the expression of genes responsible for lipid generation (Srebp-1c, Fasn, Cd36, Scd1, Dgat1, and Pparg), which probably reduced lipid accumulation induced by MC-LR. Analogously, MC-LR increased intracellular lipid deposition in THLE-3 cells, while ASX decreased these symptoms by down-regulating the expression of key genes in the lipid synthesis pathway. Our results implied that ASX played a crucial part in lipid synthesis and effectively alleviated MC-LR-induced lipid metabolism dysregulation. ASX might be developed as a novel protectant against hepatic impairment and lipid metabolic dysregulation associated with MC-LR. This study offers new insights for further management of MC-LR-related metabolic diseases.
Optimization of Embodied Energy and Construction Cost of Low-Income Housing in Urban India
Authors: Bansal Deepak , Byahut Sweta , Bansal Yashika
Published: September 2024

India is the most populous country in the world, having a population of 1.42 billion in 2022. It is urbanizing rapidly, with the present urbanization level at about 35%, which is expected to reach about 40% by 2030. There was an estimated demand of 11.22 million homes in urban India in 2017, of which 95% was in the affordable housing sector. This demand is expected to increase with the current urbanization trends. The Indian government is promoting the construction of millions of affordable houses under its ambitious Prime Minister’s Housing Program. These houses are planned, designed, and constructed using local materials and techniques, considering local climatic, geological, hazard, and socio-economic conditions. We examined the 30 most commonly applied housing typologies to determine which typologies and materials have minimum embodied energy and construction costs. The results indicate that load-bearing housing construction of up to three stories, with a plinth–carpet area ratio of 1.31, constructed with any of the blocks-based masonry techniques, has the lowest embodied energy and construction cost, and houses with a plinth–carpet area ratio of 1.51 have the highest. Further, houses constructed with Hollow CC block masonry have the lowest embodied energy, and HF Fly Ash block-based masonry has the lowest construction cost.
Sputnik V-Induced Antibodies against SARS-CoV-2 Variants during the Dissemination of the Gamma Variant in Venezuela
Authors: Franco Christopher , Cornejo Alejandro , Rodríguez Mariajosé , García Alexis , Belisario Inirida , Mayora Soriuska , Garzaro Domingo José , Jaspe Rossana Celeste , Hidalgo Mariana , Parra Nereida , Liprandi Ferdinando , Zambrano José Luis , Rangel Héctor Rafael , Pujol Flor Helene
Published: September 2024

The COVID-19 pandemic was characterized by the emergence and succession of SARS-CoV-2 variants able to evade the antibody response induced by natural infection and vaccination. To evaluate the IgG reactivity and neutralizing capacity of the serum of individuals vaccinated with Sputnik V (105 volunteers vaccinated) against different viral variants. IgG reactivity to the Spike protein (S) was evaluated by ELISA. A plaque reduction neutralization test was performed using different viral variant isolates. At 42 days post-vaccination, the frequency of recognition and reactivity to the S protein of the Omicron variant was lower compared to that of the other variants. In general, a higher average neutralization titer was seen against the ancestral variant compared to the variants, especially Omicron. However, some sera exhibited a higher neutralization titer to the Gamma variant compared to the ancestral variant, suggesting unapparent exposure during the clinical trial. Antibodies induced by Sputnik V can recognize, persist, and neutralize SARS-CoV-2 variants, with Omicron being the one that best evades this response. These results represent a unique report on the humoral response induced by a globally lesser-studied vaccine in terms of efficacy and immune escape, offering insights into developing vaccines targeting unknown coronaviruses.
The Autonomous Fusion Activity of Human Cytomegalovirus Glycoprotein B Is Regulated by Its Carboxy-Terminal Domain
Authors: Reuter Nina , Kropff Barbara , Chen Xiaohan , Britt William J. , Sticht Heinrich , Mach Michael , Thomas Marco
Published: September 2024

The human cytomegalovirus (HCMV) glycoprotein B (gB) is the viral fusogen required for entry into cells and for direct cell-to-cell spread of the virus. We have previously demonstrated that the exchange of the carboxy-terminal domain (CTD) of gB for the CTD of the structurally related fusion protein G of the vesicular stomatitis virus (VSV-G) resulted in an intrinsically fusion-active gB variant (gB/VSV-G). In this present study, we employed a dual split protein (DSP)-based cell fusion assay to further characterize the determinants of fusion activity in the CTD of gB. We generated a comprehensive library of gB CTD truncation mutants and identified two mutants, gB-787 and gB-807, which were fusion-competent and induced the formation of multinucleated cell syncytia in the absence of other HCMV proteins. Structural modeling coupled with site-directed mutagenesis revealed that gB fusion activity is primarily mediated by the CTD helix 2, and secondarily by the recruitment of cellular SH2/WW-domain-containing proteins. The fusion activity of gB-807 was inhibited by gB-specific monoclonal antibodies (MAbs) targeting the antigenic domains AD-1 to AD-5 within the ectodomain and not restricted to MAbs directed against AD-4 and AD-5 as observed for gB/VSV-G. This finding suggested a differential regulation of the fusion-active conformational state of both gB variants. Collectively, our findings underscore a pivotal role of the CTD in regulating the fusogenicity of HCMV gB, with important implications for understanding the conformations of gB that facilitate membrane fusion, including antigenic structures that could be targeted by antibodies to block this essential step in HCMV infection.
Safety of COVID-19 Vaccines among People with History of Allergy: A European Active Surveillance Study
Authors: Luxi Nicoletta , Ciccimarra Francesco , Bellitto Chiara , Raethke Monika , van Hunsel Florence , Lieber Thomas , Mulder Erik , L’Abbate Luca , Marques Francisco Batel , Furci Fabiana , Farcas Andreea , Giele-Eshuis Janneke , Morton Kathryn , Sonderlichová Simona , Thurin Nicolas H. , Villalobos Felipe , Riefolo Fabio , Sturkenboom Miriam C. , Trifirò Gianluca
Published: September 2024

Background: Conventional vaccines rarely cause severe allergic reactions. However, the rapid development and approval of COVID-19 vaccines left limited initial data on their adverse reactions, particularly in individuals with a history of allergy. The aim of this study was to assess and compare the safety profile of different doses and brands of COVID-19 vaccines in subjects with a history of allergy vs. those without a history of allergy. Methods: From February 2021 to February 2023, a web-based prospective study gathered vaccinee-reported outcomes using electronic questionnaires across eleven European countries. Baseline and up to six follow-up questionnaires captured data on vaccinee demographics, as well as both solicited and unsolicited adverse reactions. Results: Overall, 3476 vaccinees with a history of allergy were matched with 13,872 vaccinees from the general population at the first vaccination cycle and were included in the analysis. A total of 825 vaccinees with a history of allergy who had received a booster dose, matched to 3297 vaccinees from the general population, were included in the analysis. Higher rates of ADRs occurred after the first vaccination cycle compared to after the booster dose (64–91% vs. 56–79%). However, most reported ADRs were solicited and not serious, and no case of anaphylaxis was reported. Women and vaccinees with a history of allergy reported ADRs more frequently than men and the matched controls, respectively. Compared to other COVID-19 vaccines, a higher proportion of vaccinees experiencing at least one ADR following their first vaccination cycle was observed with Comirnaty and Vaxzevria. Statistically significant differences were observed among the study cohorts for median TTO after the second dose, and for median TTR following the first vaccination cycle and booster dose (p < 0.001). Conclusions: Typically, any drug or vaccine use carries a risk of severe allergic reactions, yet the benefits of vaccination generally outweigh these potential risks, as shown with the COVID-19 vaccines.
Pediatric Oncology Providers’ HPV Vaccine Knowledge, Attitude, Self-Efficacy, and Practice after Communication Training: A Comparison with a National Survey
Authors: Teixeira Rejane A. , Grimes Allison , Embry Leanne , Aguilar Christine , Shay L. Aubree
Published: September 2024

Background/Objectives: Human papillomavirus (HPV) vaccinations prevent HPV infection and related cancers. Despite being at higher risk of secondary cancers linked to HPV, childhood cancer survivors (CCS) are undervaccinated. This study aimed to compare pediatric oncology providers’ knowledge, attitudes, self-efficacy, and practices regarding HPV vaccination among those who participated in a multilevel educational HPV vaccine program with those of a national sample of oncology providers. Methods: Between February and March 2023, 39 providers from five pediatric oncology clinics in Texas completed online surveys, assessing knowledge about CCS risk for HPV-related cancers, attitudes towards the HPV vaccine, and confidence in recommending the vaccine to CCS. The results were compared with a national survey of providers conducted in 2019 (n = 195). Results: The findings showed that providers who participated in our program had greater knowledge of CCS increased risk for HPV-related cancers (96% vs. 38%; p < 0.001); greater confidence in discussing and recommending the HPV vaccine (100% vs. 66%, p < 0.001) and addressing parental concerns (100% vs. 69%, p < 0.001); and a more positive attitude about oncology providers than general pediatricians, recommending (96% vs. 71%; p = 0.006) and administering the HPV vaccine to CCS (96% vs. 53%, p < 0.001). Conclusion: This study underscores the importance of educating oncology providers about the increased risk of CCS and improving their self-efficacy to recommend the HPV vaccine, promoting vaccination in the oncology setting.
Enhanced Antimicrobial Peptide Response Following Bacillus Calmette–Guerin Vaccination in Elderly Individuals
Authors: Pandiarajan Arul Nancy , Kumar Nathella Pavan , Rajamanickam Anuradha , Bhavani Perumal Kannabiran , Jeyadeepa Bharathi , Selvaraj Nandhini , Asokan Dinesh , Tripathy Srikanth , Padmapriyadarsini Chandrasekharan , Babu Subash
Published: September 2024

Background: Antimicrobial peptides are an important component of host defense against Mycobacterium tuberculosis. However, the ability of BCG to induce AMPs as part of its mechanism of action has not been investigated in detail. Methods: We investigated the impact of Bacillus Calmette–Guerin (BCG) vaccination on circulating plasma levels and TB-antigen stimulated plasma levels of AMPs in a healthy elderly population. We assessed the association of AMPs, including Human Beta Defensin 2 (HBD-2), Human Neutrophil Peptide 1-3 (HNP1-3), Granulysin, and Cathelicidin (LL37), in circulating plasma and TB-antigen stimulated plasma (using IGRA supernatants) at baseline (pre-vaccination) and at Month 1 and Month 6 post vaccination. Results: Post BCG vaccination, both circulating plasma levels and TB-antigen stimulated plasma levels of AMPs significantly increased at Month 1 and Month 6 compared to pre-vaccination levels in the elderly population. However, the association of AMP levels with latent TB (LTB) status did not exhibit statistical significance. Conclusion: Our findings indicate that BCG vaccination is linked to heightened circulating levels of AMPs in the elderly population, which are also TB-antigen-specific. This suggests a potential mechanism underlying the immune effects of BCG in enhancing host defense against TB.
Time Series Analysis to Estimate the Volume of Drinking Water Consumption in the City of Meoqui, Chihuahua, Mexico
Authors: Legarreta-González Martín Alfredo , Meza-Herrera César A. , Rodríguez-Martínez Rafael , Chávez-Tiznado Carlos Servando , Véliz-Deras Francisco Gerardo
Published: September 2024

Water is a vital resource for sustaining life and for numerous processes within the transformation industry. It is a finite resource, albeit one that can be renewed, and thus sustainable management is imperative. To achieve this objective, it is necessary to have the appropriate tools to assist with the planning policies for its management. This paper presents a time series analysis approach to measure and predict the pattern of water consumption by humans throughout subsectors (domestic, commercial, public sector, education, industry, and raw water) and total water consumption in Meoqui, Chihuahua, Mexico with data from 2011 to 2023, applying calibration model techniques to measure uncertainty in the forecasting. The municipality of Meoqui encompasses an area of 342 km2. The climate is semi-arid, with an average annual rainfall of 272 mm and average temperatures of 26.4 °C in summer and 9.7 °C in winter. The municipal seat, which has a population of 23,140, is supplied with water from ten wells, with an average consumption of 20 ± 579 m3 per user. The consumption of the general population indicates the existence of a seasonal autoregressive integrated moving average (SARIMA) (0,1,2)(0,0,2)12 model. (Sen’s Slope = 682.7, p < 0.001). The domestic sector exhibited the highest overall consumption, with a total volume of 17,169,009 m3 (13 ± 93). A SARIMA (2,1,0)(2,0,0)12 model was estimated, with a Sen’s slope of 221.65 and a p-value of less than 0.001. The second-largest consumer of total water was the “raw water” sector, which consumed 5,124,795 (30,146 ± 35,841) m3 and exhibited an SARIMA (0,1,1)(2,0,0)12 model with no statistically significant trend. The resulting models will facilitate the company’s ability to define water resource management strategies in a sustainable manner, in alignment with projected consumption trends.