bluuebunny/crossref_metadata_2025 huggingface dataset:
Independent Associated SNPs at SORCS3 and Its Protein Interactors for Multiple Brain-Related Disorders and Traits
Authors: Kamran Muhammad , Laighneach Aodán , Bibi Farhana , Donohoe Gary , Ahmed Naveed , Rehman Asim Ur , Morris Derek W.
Published: February 2023

Sortilin-related vacuolar protein sorting 10 (VPS10) domain containing receptor 3 (SORCS3) is a neuron-specific transmembrane protein involved in the trafficking of proteins between intracellular vesicles and the plasma membrane. Genetic variation at SORCS3 is associated with multiple neuropsychiatric disorders and behavioural phenotypes. Here, we undertake a systematic search of published genome-wide association studies to identify and catalogue associations between SORCS3 and brain-related disorders and traits. We also generate a SORCS3 gene-set based on protein–protein interactions and investigate the contribution of this gene-set to the heritability of these phenotypes and its overlap with synaptic biology. Analysis of association signals at SORSC3 showed individual SNPs to be associated with multiple neuropsychiatric and neurodevelopmental brain-related disorders and traits that have an impact on the experience of feeling, emotion or mood or cognitive function, while multiple LD-independent SNPs were associated with the same phenotypes. Across these SNPs, alleles associated with the more favourable outcomes for each phenotype (e.g., decreased risk of neuropsychiatric illness) were associated with increased expression of the SORCS3 gene. The SORCS3 gene-set was enriched for heritability contributing to schizophrenia (SCZ), bipolar disorder (BPD), intelligence (IQ) and education attainment (EA). Eleven genes from the SORCS3 gene-set were associated with more than one of these phenotypes at the genome-wide level, with RBFOX1 associated with SCZ, IQ and EA. Functional annotation revealed that the SORCS3 gene-set is enriched for multiple ontologies related to the structure and function of synapses. Overall, we find many independent association signals at SORCS3 with brain-related disorders and traits, with the effect possibly mediated by reduced gene expression, resulting in a negative impact on synaptic function.
Emerging Therapeutic Strategies for Diffuse Intrinsic Pontine Glioma: A Systematic Review
Authors: Farrukh Shahrukh , Habib Shagufta , Rafaqat Amna , Sarfraz Zouina , Sarfraz Azza , Sarfraz Muzna , Robles-Velasco Karla , Felix Miguel , Cherrez-Ojeda Ivan
Published: February 2023

Background: Of all central nervous systems tumors, 10–20% are located in the brainstem; diffuse intrinsic pontine glioma (DIPG) is diagnosed in 80% of them. With over five decades of clinical trial testing, there are no established therapeutic options for DIPG. This research article aims to collate recent clinical trial data and provide a landscape for the most promising therapies that have emerged in the past five years. Methods: PubMed/MEDLINE, Web of Science, Scopus, and Cochrane were systematically searched using the following keywords: Diffuse intrinsic pontine glioma, Pontine, Glioma, Treatment, Therapy, Therapeutics, curative, and/or Management. Both adult and pediatric patients with newly diagnosed or progressive DIPG were considered in the clinical trial setting. The risk of bias was assessed using the ROBINS-I tool. Results: A total of 22 trials were included reporting the efficacy and safety outcomes among patients. First, five trials reported outcomes of blood–brain barrier bypass via single or repeated-dose intra-arterial therapy or convection-enhanced delivery. Second, external beam radiation regimens were assessed for safety and efficacy in three trials. Third, four trials administered intravenous treatment without using chemotherapeutic regimens. Fourth, eight trials reported the combinations of one or more chemotherapeutic agents. Fifth, immunotherapy was reported in two trials in an adjuvant monotherapy in the post-radiotherapy setting. Conclusion: This research article captures a clinical picture of the last five years of the direction toward which DIPG research is heading. The article finds that re-irradiation may prolong survival in patients with progressive DIPG; it also instills that insofar palliative radiotherapy has been a key prognostic choice.
Social Suffering: Indigenous Peoples’ Experiences of Accessing Mental Health and Substance Use Services
Authors: Smye Victoria , Browne Annette J. , Josewski Viviane , Keith Barbara , Mussell William
Published: February 2023

In this paper, we present findings from a qualitative study that explored Indigenous people’s experiences of mental health and addictions care in the context of an inner-city area in Western Canada. Using an ethnographic design, a total of 39 clients accessing 5 community-based mental health care agencies were interviewed, including 18 in-depth individual interviews and 4 focus groups. Health care providers also were interviewed (n = 24). Data analysis identified four intersecting themes: normalization of social suffering; re-creation of trauma; the challenge of reconciling constrained lives with harm reduction; and mitigating suffering through relational practice. The results highlight the complexities of experiences of accessing systems of care for Indigenous people marginalized by poverty and other forms of social inequity, and the potential harms that arise from inattention to the intersecting social context(s) of peoples’ lives. Service delivery that aims to address the mental health concerns of Indigenous people must be designed with awareness of, and responsiveness to, the impact of structural violence and social suffering on peoples’ lived realities. A relational policy and policy lens is key to alleviate patterns of social suffering and counter the harms that are unwittingly created when social suffering is normalized.
Development of a Daily Living Self-Efficacy Scale for Older Adults in Japan
Authors: Suzuki Mizue , Shigeta Masahiro , Kanamori Takuya , Yokomichi Marika , Uchiyama Masayo , Inagaki Keigo , Naito Tomoyoshi , Ooshiro Hajime , Asai Yatami
Published: February 2023

Objectives: Older adults tend to experience decreased enjoyment and fulfillment in life, social interactions, and independent living, with aging. These situations often result in lower levels of daily living self-efficacy in activities, which is one of the factors resulting in a decline in the quality of life (QOL) among older individuals. For this reason, interventions that help maintain daily living self-efficacy among older adults may also help maintain a good QOL. The objective of this study was to develop a daily living self-efficacy scale for the elderly that can be used to evaluate the effects of interventions aimed at enhancing self-efficacy. Methods: An expert meeting involving specialists in dementia treatment and care was held, to prepare a draft for a daily living self-efficacy scale. In the meeting, previous studies on self-efficacy among older adults, which were collected in advance, were reviewed, and the experiences of the specialists were discussed. Based on the reviews and discussions, a draft of a daily living self-efficacy scale comprising 35 items was prepared. This study on daily living self-efficacy was conducted from January 2021 to October 2021. The internal consistency and concept validity of the scale were evaluated based on the assessment data. Results: The mean age ± standard deviation of the 109 participants was 84.2 ± 7.3 years. The following five factors were extracted based on factor analysis: Factor 1, “Having peace of mind”; Factor 2, “Maintaining healthy routines and social roles”; Factor 3, “Taking personal care of oneself”; Factor 4, “Rising to the challenge”; and Factor 5, “Valuing enjoyment and relationships with others”. The Cronbach’s alpha coefficient exceeded 0.7, thereby suggesting sufficiently high internal consistency. Covariance structure analysis confirmed sufficiently high concept validity. Conclusions: The scale developed in this study was confirmed to be sufficiently reliable and valid, and when used during dementia treatment and care to assess the levels of daily living self-efficacy among older adults, it is expected to contribute to the improvement of QOL among older adults.
Sex Education and Comprehensive Health Education in the Future of Educational Professionals
Authors: Jiménez-Ríos Francisco Javier , González-Gijón Gracia , Martínez-Heredia Nazaret , Amaro Agudo Ana
Published: February 2023

This work shows an approach to comprehensive sexual education as an anthropological key to promoting health in the self-realisation of future education professionals. Comprehensive sexual education and health form a system. To carry out this study, we have analysed the opinion that students of the Faculty of Education Sciences of the University of Granada (Spain) have about the comprehensive sexual education received and the importance of this training in their professional practice. For this purpose, we used a quantitative and exploratory research design, using a questionnaire as an instrument for collecting information with a sample of 293 students. The results show that students have received poor sex education, along with the belief that education professionals do not receive proper and organised training in sex education. We can conclude that the majority of respondents consider sex education to be a recognised right, highlighting the importance of education professionals receiving proper training in sex education at university, where content related to respect, education for equality, and sexual health is prioritised. Sexuality constitutes the fundamental anthropological structure: comprehensive sexual education is a source of personal (corporal, psychic, spiritual) and social health, hence the importance of educating in comprehensive sexuality.
Mental Health Literacy and Stigma in a Municipality in the North of Portugal: A Cross-Sectional Study
Authors: Simões de Almeida Raquel , Trigueiro Maria João , Portugal Paula , de Sousa Sara , Simões-Silva Vítor , Campos Filipa , Silva Maria , Marques António
Published: February 2023

Portugal has Europe’s second-highest prevalence of psychiatric illnesses, and this is the reason why mental health literacy (MHL) and stigma should be addressed. This study aimed to investigate the mental health literacy and stigma levels among different groups of people from Póvoa de Varzim, a municipality in the north of Portugal. Students, retired people, and professionals (education, social, and healthcare fields) were recruited using a convenience sample from June to November 2022. Participants’ MHL levels were evaluated using the Mental Health Promoting Knowledge Scale (MHPK), Mental Health Literacy Measure (MHLM) and Mental Health Knowledge Schedule (MAKS). Stigma levels were evaluated using Community Attitudes towards Mental Illness (CAMI) and the Reported and Intended Behaviour Scale (RIBS). A total of 928 questionnaires were filed. The respondents included 65.70% of women, a mean age of 43.63 (±26.71) years and 9.87 (±4.39) years of school education. MHL increased with age, education level and was higher in women (p < 0.001). A higher level of MHL was seen in health professionals (p < 0.001). Findings revealed that older people stigmatized people with mental illness more (p < 0.001), and the female gender stigmatize less (p < 0.001). In addition, results showed that stigma decreased with higher mental health literacy (r between 0.11 and 0.38; p < 0.001). To conclude, specific campaigns that promote mental health literacy should be tailored to specific profiles within this population to address those that have more stigma.
Experience and Satisfaction with a Family-Based Physical Activity Intervention Using Activity Trackers and Apps: A Qualitative Study
Authors: Schoeppe Stephanie , Waters Kim , Salmon Jo , Williams Susan L. , Power Deborah , Alley Stephanie , Rebar Amanda L. , Hayman Melanie , Duncan Mitch J. , Vandelanotte Corneel
Published: February 2023

Wearable activity trackers and smartphone apps have been shown to increase physical activity in children and adults. However, interventions using activity trackers and apps have rarely been tested in whole families. This study examined the experience and satisfaction with an activity tracker and app intervention (Step it Up Family) to increase physical activity in whole families. Telephone interviews were conducted with Queensland-based families (n = 19) who participated in the Step it Up Family intervention (N = 40, single-arm, pre/post feasibility study) in 2017/2018. Using commercial activity trackers combined with apps, the intervention included an introductory session, individual and family-level goal setting, self-monitoring, family step challenges, and weekly motivational text messages. Qualitative content analysis was conducted to identify themes, categories and sub-categories. In summary, parents reported that children were engaged with the activity tracker and app features to reach their daily step goals. Some technical difficulties were experienced with app navigation, syncing of activity tracker data, and tracker band discomfort. Although families liked that the weekly text messages reminded them to be active, they did not find them very motivating. Using text messages for physical activity motivation in families requires further testing. Overall, the intervention was well-received by families for increasing physical activity motivation.
Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor
Authors: Yildizhan Yagmur , Driessens Kaat , Tsao Hong Shen Kevin , Boiy Robin , Thomas Debby , Geukens Nick , Hendrix An , Lammertyn Jeroen , Spasic Dragana
Published: February 2023

Extracellular vesicles (EVs) have attracted great attention as potential biomarkers for cancer diagnostics. Although several technologies have been developed for EV detection, many of them are still not applicable to clinical settings as they rely on complex EV isolation processes, while lacking sensitivity, specificity or standardization. To solve this problem, we have developed a sensitive breast cancer-specific EV detection bioassay directly in blood plasma using a fiber-optic surface plasmon resonance (FO-SPR) biosensor, previously calibrated with recombinant EVs. First, we established a sandwich bioassay to detect SK-BR-3 EVs by functionalizing the FO-SPR probes with anti-HER2 antibodies. A calibration curve was built using an anti-HER2/Banti-CD9 combination, resulting in an LOD of 2.1 × 107 particles/mL in buffer and 7 × 108 particles/mL in blood plasma. Next, we investigated the potential of the bioassay to detect MCF7 EVs in blood plasma using an anti-EpCAM/Banti-mix combination, obtaining an LOD of 1.1 × 10 8 particles/mL. Finally, the specificity of the bioassay was proven by the absence of signal when testing plasma samples from 10 healthy people unknown to be diagnosed with breast cancer. The remarkable sensitivity and specificity of the developed sandwich bioassay together with the advantages of the standardized FO-SPR biosensor highlight outstanding potential for the future of EV analysis.
‘Toxic Masculinity’: What Is Known about the Role of Androgen Receptors in Head and Neck Squamous Cell Carcinoma
Authors: Čonkaš Josipa , Sabol Maja , Ozretić Petar
Published: February 2023

Head and neck squamous cell carcinoma (HNSCC), the most prevalent cancer in the head and neck region, develops from the mucosal epithelium of the upper aerodigestive tract. Its development directly correlates with alcohol and/or tobacco consumption and infection with human papillomavirus. Interestingly, the relative risk for HNSCC is up to five times higher in males, so it is considered that the endocrine microenvironment is another risk factor. A gender-specific risk for HNSCC suggests either the existence of specific risk factors that affect only males or that females have defensive hormonal and metabolic features. In this review, we summarized the current knowledge about the role of both nuclear and membrane androgen receptors (nAR and mARs, respectively) in HNSCC. As expected, the significance of nAR is much better known; it was shown that increased nAR expression was observed in HNSCC, while treatment with dihydrotestosterone increased proliferation, migration, and invasion of HNSCC cells. For only three out of five currently known mARs—TRPM8, CaV1.2, and OXER1—it was shown either their increased expression in various types of HNSCC or that their increased activity enhanced the migration and invasion of HNSCC cells. The primary treatments for HNSCC are surgery and radiotherapy, but targeted immunotherapies are on the rise. On the other hand, given the evidence of elevated nAR expression in HNSCC, this receptor represents a potential target for antiandrogen therapy. Moreover, there is still plenty of room for further examination of mARs’ role in HNSCC diagnosis, prognosis, and treatment.
Intrathecal versus Peripheral Inflammatory Protein Profile in MS Patients at Diagnosis: A Comprehensive Investigation on Serum and CSF
Authors: Pezzini Francesco , Pisani Annalisa , Mazziotti Valentina , Marastoni Damiano , Tamanti Agnese , Borroni Edilio , Magon Stefano , Zinnhardt Bastian , Magliozzi Roberta , Calabrese Massimiliano
Published: February 2023

Intrathecal inflammation plays a key role in the pathogenesis of multiple sclerosis (MS). To better elucidate its relationship with peripheral inflammation, we investigated the correlation between cerebrospinal fluid (CSF) and serum levels of 61 inflammatory proteins. Paired CSF and serum samples were collected from 143 treatment-naïve MS patients at diagnosis. A customized panel of 61 inflammatory molecules was analyzed by a multiplex immunoassay. Correlations between serum and CSF expression levels for each molecule were performed by Spearman’s method. The expression of sixteen CSF proteins correlated with their serum expression (p-value < 0.001): only five molecules (CXCL9, sTNFR2, IFNα2, Pentraxin-3, and TSLP) showed a Rho value >0.40, suggesting moderate CSF/serum correlation. No correlation between inflammatory serum patterns and Qalb was observed. Correlation analysis of serum expression levels of these sixteen proteins with clinical and MRI parameters pinpointed a subset of five molecules (CXCL9, sTNFR2, IFNα2, IFNβ, and TSLP) negatively correlating with spinal cord lesion volume. However, following FDR correction, only the correlation of CXCL9 remained significant. Our data support the hypothesis that the intrathecal inflammation in MS only partially associates with the peripheral one, except for the expression of some immunomodulators that might have a key role in the initial MS immune response.
A Proposed Methodology to Deal with the Impact of In Vitro Cellular Matrix on the Analytical Performances of a Targeted Metabolomic LC-HRMS Method
Authors: Guitton Jérôme , Gavotto Floriane , Cros-Perrial Emeline , Jordheim Lars Petter , Machon Christelle
Published: February 2023

Performances of metabolomic methods have been widely studied on biological matrices such as serum, plasma, and urine; but much less on in vitro cell extracts. While the impact of cell culture and sample preparation on results are well-described, the specific effect of the in vitro cellular matrix on the analytical performance remains uncertain. The aim of the present work was to study the impact of this matrix on the analytical performance of an LC-HRMS metabolomic method. For this purpose, experiments were performed on total extracts from two cell lines (MDA-MB-231 and HepaRG) using different cell numbers. Matrix effects, carryover, linearity, and variability of the method were studied. Results showed that the performances of the method depend on the nature of the endogenous metabolite, the cell number, and the nature of the cell line. These three parameters should, therefore, be considered for the processing of experiments and the interpretation of results depending on whether the study focuses on a limited number of metabolites or aims to establish a metabolic signature.
Distinct Conformations of SARS-CoV-2 Omicron Spike Protein and Its Interaction with ACE2 and Antibody
Authors: Lee Myeongsang , Major Marian , Hong Huixiao
Published: February 2023

Since November 2021, Omicron has been the dominant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant that causes the coronavirus disease 2019 (COVID-19) and has continuously impacted human health. Omicron sublineages are still increasing and cause increased transmission and infection rates. The additional 15 mutations on the receptor binding domain (RBD) of Omicron spike proteins change the protein conformation, enabling the Omicron variant to evade neutralizing antibodies. For this reason, many efforts have been made to design new antigenic variants to induce effective antibodies in SARS-CoV-2 vaccine development. However, understanding the different states of Omicron spike proteins with and without external molecules has not yet been addressed. In this review, we analyze the structures of the spike protein in the presence and absence of angiotensin-converting enzyme 2 (ACE2) and antibodies. Compared to previously determined structures for the wildtype spike protein and other variants such as alpha, beta, delta, and gamma, the Omicron spike protein adopts a partially open form. The open-form spike protein with one RBD up is dominant, followed by the open-form spike protein with two RBD up, and the closed-form spike protein with the RBD down. It is suggested that the competition between antibodies and ACE2 induces interactions between adjacent RBDs of the spike protein, which lead to a partially open form of the Omicron spike protein. The comprehensive structural information of Omicron spike proteins could be helpful for the efficient design of vaccines against the Omicron variant.
CircRNA Profiling of Skeletal Muscle in Two Pig Breeds Reveals CircIGF1R Regulates Myoblast Differentiation via miR-16
Authors: Li Meng , Zhang Na , Li Jiao , Ji Mengting , Zhao Tianzhi , An Jiaqi , Cai Chunbo , Yang Yang , Gao Pengfei , Cao Guoqing , Guo Xiaohong , Li Bugao
Published: February 2023

Muscle development is closely related to meat quality and production. CircRNAs, with a closed-ring structure, have been identified as a key regulator of muscle development. However, the roles and mechanisms of circRNAs in myogenesis are largely unknown. Hence, in order to unravel the functions of circRNAs in myogenesis, the present study explored circRNA profiling in skeletal muscle between Mashen and Large White pigs. The results showed that a total of 362 circRNAs, which included circIGF1R, were differentially expressed between the two pig breeds. Functional assays showed that circIGF1R promoted myoblast differentiation of porcine skeletal muscle satellite cells (SMSCs), while it had no effect on cell proliferation. In consideration of circRNA acting as a miRNA sponge, dual-luciferase reporter and RIP assays were performed and the results showed that circIGF1R could bind miR-16. Furthermore, the rescue experiments showed that circIGF1R could counteract the inhibitory effect of miR-16 on cell myoblast differentiation. Thus, circIGF1R may regulate myogenesis by acting as a miR-16 sponge. In conclusion, this study successfully screened candidate circRNAs involved in the regulation of porcine myogenesis and demonstrated that circIGF1R promotes myoblast differentiation via miR-16, which lays a theoretical foundation for understanding the role and mechanism of circRNAs in regulating porcine myoblast differentiation.
Molecular Mechanisms of Hepatotoxicity
Authors: Messina Antonietta , Duclos-Vallée Jean-Charles
Published: February 2023

Drug-induced liver injury, also known as drug-induced hepatotoxicity (DILI), is a major cause of medicine withdrawal (prescription or over-the-counter) from the market [...]
Comparison of the Germination Conditions of Two Large-Spore Microsporidia Using Potassium and Sodium Ion Solutions
Authors: Imura Yuji , Nakamura Haruka , Arai Reina , Hatakeyama Yoshinori
Published: February 2023

The germination of a microsporidian polar tube generally occurs under alkaline conditions. Typically, microsporidian spores can be stored in physiological salt solution for short periods. However, because of differences in the lodging area, the requirements may not always be uniform. In fact, Trachipleistophora sp. OSL-2012-10 (nomen nudum Trachipleistophora haruka) germinated when preserved in physiological salt solution. In this study, the germination characteristics of the large-spore microsporidia Trachipleistophora sp. FOA-2014-10 and Vavraia sp. YGSL-2015-13 were compared with those of Trachipleistophora sp. OSL-2012-10; moreover, we investigated whether these characteristics are specific to these microsporidia. We found that both microsporidia germinated in physiological salt solution. These differences in germination rates were affected by the preservation solution and temperature.
Expression Pattern and Molecular Mechanism of Oxidative Stress-Related Genes in Myocardial Ischemia–Reperfusion Injury
Authors: Wu Jiahe , Luo Jingyi , Cai Huanhuan , Li Chenze , Lei Zhe , Lu Yi , Ni Lihua , Cao Jianlei , Cheng Bo , Hu Xiaorong
Published: February 2023

(1) Background: The molecular mechanism of oxidative stress-related genes (OSRGs) in myocardial ischemia–reperfusion injury (MIRI) has not been fully elucidated. (2) Methods: Differential expression analysis, enrichment analysis, and PPI analysis were performed on the MIRI-related datasets GSE160516 and GSE61592 to find key pathways and hub genes. OSRGs were obtained from the Molecular Signatures Database (MSigDB). The expression pattern and time changes of them were studied on the basis of their raw expression data. Corresponding online databases were used to predict miRNAs, transcription factors (TFs), and therapeutic drugs targeting common differentially expressed OSRGs. These identified OSRGs were further verified in the external dataset GSE4105 and H9C2 cell hypoxia–reoxygenation (HR) model. (3) Results: A total of 134 DEGs of MIRI were identified which were enriched in the pathways of “immune response”, “inflammatory response”, “neutrophil chemotaxis”, “phagosome”, and “platelet activation”. Six hub genes and 12 common differentially expressed OSRGs were identified. A total of 168 miRNAs, 41 TFs, and 21 therapeutic drugs were predicted targeting these OSRGs. Lastly, the expression trends of Aif1, Apoe, Arg1, Col1a1, Gpx7, and Hmox1 were confirmed in the external dataset and HR model. (4) Conclusions: Aif1, Apoe, Arg1, Col1a1, Gpx7, and Hmox1 may be involved in the oxidative stress mechanism of MIRI, and the intervention of these genes may be a potential therapeutic strategy.
Treatment of Skull Base Diseases: A Multidisciplinary Challenge
Authors: Franz Leonardo , Zanoletti Elisabetta , Nicolai Piero , Ferrari Marco
Published: February 2023

The skull base has always been regarded as a frontier by surgeons and radiation oncologists since it represents the interface between the intracranial and the extracranial compartment and hosts several critical anatomical structures with an extremely complex and close relationship [...]
Response to Peptide Receptor Radionuclide Therapy in Pheocromocytomas and Paragangliomas: A Systematic Review and Meta-Analysis
Authors: Marretta Antonella Lucia , Ottaiano Alessandro , Iervolino Domenico , Bracigliano Alessandra , Clemente Ottavia , Di Gennaro Francesca , Tafuto Roberto , Santorsola Mariachiara , Lastoria Secondo , Tafuto Salvatore
Published: February 2023

Introduction. Peptide receptor radionuclide therapy (PRRT) with 177Lu-DOTATATE and 90Y-DOTATOC showed efficacy in the metastatic setting of pheocromocytomas (PCCs) and paragangliomas (PGLs) where no standard therapies have been established. Background. A search of peer-reviewed and English articles reporting on 177Lu-DOTATATE and 90Y-DOTATOC efficacy was performed through Medline and Scopus. A subsequent meta-analysis was performed to evaluate the pooled effect size on disease control rate (DCR) with PRRT. Secondary endpoints were description of patients’ genetic characteristics, hematologic toxicity, and time-to-outcome. The pooled effect was estimated with both a mixed-effects model and a random-effects model. Results. Twelve studies met the criteria for this meta-analysis: ten with 177Lu- and two with 90Y-PRRTs (213 patients). The largest one included 46 patients. Median ages ranged from 32.5 to 60.4 years. When reported, mutations of SDHB were the most frequent genetic alterations. The pooled DCRs were 0.83 (95% CI: 0.75–0.88) and 0.76 (95% CI: 0.56–0.89) for 177Lu- and 90Y-PRRT, respectively. The pooled DCR for PRRT was 0.81 (95% CI: 0.74–0.87). Conclusions. We report an updated and solid estimate of DCR achieved with 177Lu- and 90Y-PRRT in PCCs and PGLs, showing that these therapies can be considered in the multidisciplinary treatment of PCCs and PGLs as alternatives to I-131 MIBG and chemotherapy.
Urethral Injuries: Diagnostic and Management Strategies for Critical Care and Trauma Clinicians
Authors: Patel Anish B. , Osterberg E. Charles , Satarasinghe Praveen N. , Wenzel Jessica L. , Akbani Sabah T. , Sahi Saad L. , Emigh Brent J. , Wolf J. Stuart , Brown Carlos V. R.
Published: February 2023

Urologic trauma is a well-known cause of urethral injury with a range of management recommendations. Retrograde urethrogram remains the preferred initial diagnostic modality to evaluate a suspected urethral injury. The management thereafter varies based on mechanism of injury. Iatrogenic urethral injury is often caused by traumatic catheterization and is best managed by an attempted catheterization performed by an experienced clinician or suprapubic catheter to maximize urinary drainage. Penetrating trauma, most commonly associated with gunshot wounds, can cause either an anterior and/or posterior urethral injury and is best treated with early operative repair. Blunt trauma, most commonly associated with straddle injuries and pelvic fractures, can be treated with either early primary endoscopic realignment or delayed urethroplasty after suprapubic cystostomy. With any of the above injury patterns and treatment options, a well thought out and regimented follow-up with a urologist is of utmost importance for accurate assessment of outcomes and appropriate management of complications.
Polycystic Ovary Syndrome: Challenges and Possible Solutions
Authors: Che Yue , Yu Jie , Li Yu-Shan , Zhu Yu-Chen , Tao Tao
Published: February 2023

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women of reproductive age. This syndrome not only impairs female fertility but also increases the risk of obesity, diabetes, dyslipidemia, cardiovascular diseases, psychological diseases, and other health problems. Additionality, because of the high clinical heterogeneity, the current pathogenesis of PCOS is still unclear. There is still a large gap in precise diagnosis and individualized treatment. We summarize the present findings concerning the genetics, epigenetics, gut microbiota, corticolimbic brain responses, and metabolomics of the PCOS pathogenesis mechanism, highlight the remaining challenges in PCOS phenotyping and potential treatment approaches, and explain the vicious circle of intergenerational transmission of PCOS, which might provide more thoughts for better PCOS management in the future.
Identification and Hierarchy of Traditional Village Characteristics Based on Concentrated Contiguous Development—Taking 206 Traditional Villages in Hubei Province as an Example
Authors: Liu Xiaohu , Yuan Lei , Tan Gangyi
Published: February 2023

Public policy for the preservation and development of traditional villages in China has witnessed a shift. That is from the equal distribution of finances to officially recognised traditional villages to the prioritisation of the development of more potential traditional villages that stimulate the development of surrounding villages and regions. This study takes 206 traditional villages in Hubei Province as an example and proposes a method framework for ‘Feature identification, Hierarchical classification (FIHC) that combines field research, spatial geography and spatial network methods. The results of spatial geography show that traditional villages in Hubei province are unevenly distributed, mainly along the mountain ranges in the east and west, with scattered distribution in the central plains. The kernel density result reveals “3 + 2 high-density hotspot zones” (A, B, and C are high-density areas, whereas D and E are low-density areas). Furthermore, the results of the spatial network appear that the traditional villages’ ecological situation in Zone A is better than in other areas, but their transportation accessibility and economy are poor; Zone B villages’ transportation accessibility is better than in other areas; Zone C has the best economy; and three conditions of traditional villages in the D and E zones are poor. FIHC can identify villages with more development potential and stratify these villages in a multidimensional way. It is innovative and an important contribution to policymakers and planners in developing ‘phased and focused’ public policies and rural planning.
Enhancing the Gamma-Radiation-Shielding Properties of Gypsum–Lime–Waste Marble Mortars by Incorporating Micro- and Nano-PbO Particles
Authors: Alabsy Mahmoud T. , Gouda Mona M. , Abbas Mahmoud I. , Al-Balawi Shoaa Mofleh , El-Khatib Ahmed M.
Published: February 2023

In the current study, the gamma-radiation-shielding characteristics of novel gypsum–lime–waste marble-based mortars reinforced with micro-PbO and nano-PbO powders were investigated. In total, seven mortar groups, including a control mortar (named GLM), were prepared. The other groups contained10, 20, and 30 wt.% of both micro-PbO and nano-PbO as a waste marble replacement. This study aimed to explore the effect of particle size and concentrations of PbO powders on the γ-ray-shielding capability of GLM mortars. For this purpose, an HPGe detector and five standard radioactive point sources (241Am, 133Ba, 137Cs, 60Co, and 152Eu) were employed to measure different shielding parameters, including the linear attenuation coefficient (μ), mass attenuation coefficient (μm), mean free path (MFP), half-value layer (HVL), and tenth-value layer (TVL), for the prepared samples in the energy range between 59.53 keV to 1408.01 keV. On the basis of μm values, other significant shielding parameters such as effective atomic number (Zeff), effective electron density (Neff), equivalent atomic number (Zeq), and exposure buildup factor (EBF) were also computed to explore the potential usage of the proposed mortars as radiation protective materials. The results reported that the smallest HVL, TVL, and MPF, as well as the largest attenuation values, were obtained for mortars reinforced by nano-PbO compared to those containing micro-PbO. It can be concluded from the results that the mortar samples containing nano-PbO had a remarkably improved gamma-radiation-shielding ability. Thus, these mortars can be used for radiation shielding on walls in nuclear facilities to reduce the transmitted radiation dose.
Impacts of Temperature and Time on Direct Nitridation of Aluminium Powders for Preparation of AlN Reinforcement
Authors: Rogers Samuel , Dargusch Matthew , Kent Damon
Published: February 2023

Aluminium nitride (AlN) is an important technical ceramic with outstanding strength and thermal conductivity that has important applications for advanced heat sink materials and as a reinforcement for metal-based composites. In this study, we report a novel, straightforward and low-cost method to prepare AlN powder using a vacuum tube furnace for the direct nitridation of loose aluminium powder at low temperatures (down to 500 ∘C) under flowing high-purity nitrogen. Small amounts of magnesium powder (1 wt.%), combined with aluminium, promote nitridation. Here, we characterise the effects of time (up to 12 h) and temperature (490 to 560 ∘C) on nitridation with the aim to establish an effective regimen for the controlled synthesis of an aluminium nitride reinforcement powder for the production of metal matrix composites. The extent of nitridation and the morphology of the reaction products were assessed using scanning electron microscopy and X-ray diffraction analyses. AlN was detected for all nitriding temperatures ≥ 500 ∘C, with the highest yields of 80% to 85% obtained at 530 ∘C for times ≥ 1 h. At this temperature, nitridation proceeded rapidly, and there was extensive agglomeration of the reaction products making it difficult to reprocess into powder. At lower temperatures around 510 ∘C, a relatively high proportion of AlN was attained (>73% after 6 h) while retaining excellent friability so that it could be manually reprocessed to powder. The synthesised reinforcement consisted of micro- or nano-crystalline AlN comingled with metallic aluminium. The ratio of AlN and metallic aluminium can be readily controlled by varying the nitriding temperature. This provides a flexible and accessible method for the production of AlN-reinforcement powders suited to the production of metal matrix composites.
Characteristics of Surface Wave on the Unstable Interface in Gas Jet Forming
Authors: Fu Weijie , Zhang Xu , Wang Mingwei , Zhang Xinming
Published: February 2023

The surface accuracy and quality of the mirror blank determine the performance of the optical mirror after coating; therefore, it is crucial to seek a simple and effective method for mirror blank processing. The gas jet forming provides a new idea, which employs a gas jet to impinge on a liquid pool, forms a specific curved surface and solidifies it. However, due to the interface instability that occurred with a specific range of jet velocity, periodical fluctuations appear on the interface and affect the forming of the mirror blank. In this study, the amplitude and transfer period of surface waves in the air gas/dimethyl silicone oil system are taken as the research objects. After conducting orthogonal experiments, this study uses a high-speed camera to record the generation of the surface wave and the fluctuation of the gas–liquid interface during its transfer. Subsequently, this study analyses the influences of jet velocity and viscosity of dimethyl silicone oil on the amplitude and transfer period of the surface wave. In the following experiments, multiple groups of parameters were selected in the range of jet velocity of 11~13 m/s and dimethyl silicone oil viscosity of 500~2000 Pa·s. This study measures the interface morphology with different parameters using the method of image recognition. Finally, using the measured data, this study establishes the calculation model of surface wave amplitude and transfer period, realizing the study of the characteristics of surface waves on the unstable interface. This research can be used to enhance the forming accuracy of the gas jet-forming method.
Malware Detection Based on the Feature Selection of a Correlation Information Decision Matrix
Authors: Lu Kai , Cheng Jieren , Yan Anli
Published: February 2023

Smartphone apps are closely integrated with our daily lives, and mobile malware has brought about serious security issues. However, the features used in existing traffic-based malware detection techniques have a large amount of redundancy and useless information, wasting the computational resources of training detection models. To overcome this drawback, we propose a feature selection method; the core of the method involves choosing selected features based on high irrelevance, thereby removing redundant features. Furthermore, artificial intelligence has implemented malware detection and achieved outstanding detection ability. However, almost all malware detection models in deep learning include pooling operations, which lead to the loss of some local information and affect the robustness of the model. We also propose designing a malware detection model for malicious traffic identification based on a capsule network. The main difference between the capsule network and the neural network is that the neuron outputs a scalar, while the capsule outputs a vector. It is more conducive to saving local information. To verify the effectiveness of our method, we verify it from three aspects. First, we use four popular machine learning algorithms to prove the effectiveness of the proposed feature selection method. Second, we compare the capsule network with the convolutional neural network to prove the superiority of the capsule network. Finally, we compare our proposed method with another state-of-the-art malware detection technique; our accuracy and recall increased by 9.71% and 20.18%, respectively.
Black Widow Optimization Algorithm Used to Extract the Parameters of Photovoltaic Cells and Panels
Authors: Madhiarasan Manoharan , Cotfas Daniel T. , Cotfas Petru A.
Published: February 2023

The metaheuristic algorithms and their hybridization have been utilized successfully in the past to extract the parameters of photovoltaic (PV) cells and panels. The novelty of the paper consists of proposing the black widow optimization algorithm (BWOA) for the first time to identify the parameters of the two photovoltaic cells RTC France, amorphous silicon (aSi), and two photovoltaic panels PWP201, PVM 752 GaAs. The single-diode model (SDM) and double-diode model (DDM) for analyzing the PVs are considered. The performance of the BWOA is verified using four statistical tests: the root mean square error, which is the primary tool, the mean relative error, the mean bias error, and the coefficient of determination. The research results of this study are as follows: BWOA gave the same results, or very slightly better, for RTC and PWP201 for SDM in comparison with the best algorithms from the specialized literature; for all the other cases, BWOA has substantially better results, especially for PVM 752 GaAs, where the improvements in RMSE are: 16.5%, for PWP201: 6.25%, and for aSi: 5.3%, all for the DDM; the computing time is around 2 s, which is one of the lowest durations. A consistent study is made to optimize the accuracy and computational time in function of the number of iterations and population.
Inhibition of Polymicrobial Biofilms of Candida albicans–Staphylococcus aureus/Streptococcus mutans by Fucoidan–Gold Nanoparticles
Authors: Tabassum Nazia , Khan Fazlurrahman , Kang Min-Gyun , Jo Du-Min , Cho Kyung-Jin , Kim Young-Mog
Published: February 2023

The polymicrobial proliferation and development of complex biofilm morphologies by bacterial and fungal pathogens in the host are some of the key factors contributing to the failure of antimicrobial treatments. The polymicrobial interaction of Candida albicans and some bacterial species has been extensively studied in both in vitro and in vivo model systems. Alternative strategies for disrupting polymicrobial interaction and biofilm formation are constantly needed. Among several alternative strategies, the use of nanoparticles synthesized using a natural product in the treatment of microbial infection has been considered a promising approach. The current study aimed to synthesize gold nanoparticles (AuNPs) using a natural product, fucoidan, and to test their efficacy against mono and duo combinations of fungal (Candida albicans) and bacterial (Staphylococcus aureus/Streptococcus mutans) biofilms. Several methods were used to characterize and study Fu–AuNPs, including UV-vis absorption spectroscopy, FTIR, FE-TEM, EDS, DLS, zeta potential, and XRD. The concentration-dependent inhibition of early-stage biofilms and the eradication of mature biofilms of single species of C. albicans, S. aureus, and S. mutans have been observed. Early biofilms of a dual-species combination of C. albicans and S. aureus/S. mutans were also suppressed at an increasing concentration of Fu–AuNPs. Furthermore, Fu–AuNPs significantly eradicated the established mature biofilm of mixed species. The treatment method proposed in this study, which involves the use of marine-bioinspired nanoparticles, is a promising and biocompatible agent for preventing the growth of polymicrobial biofilms of bacterial and fungal pathogens.
Ultrashort Laser Texturing of Superelastic NiTi: Effect of Laser Power and Scanning Speed on Surface Morphology, Composition and Wettability
Authors: Biffi Carlo Alberto , Fiocchi Jacopo , Rancan Marzio , Gambaro Sofia , Cirisano Francesca , Armelao Lidia , Tuissi Ausonio
Published: February 2023

Among metallic biomaterials, near-equiatomic NiTi is one of the most promising intermetallic system applicable for biomedical applications, thanks to its high biocompatibility and unique superelasticity (or pseudoelasticity), offering a complete recoverable strain up to 8%. In the prospective uses for bio-devices, the surface processing of NiTi medical components plays a fundamental role for guaranteeing both a Ti oxide passivizing layer for avoiding Ni ion release into the human body and surface morphology for controlling the cell proliferation. Mechanical polishing, thermal, chemical or electro-chemical treatments are typically performed for surface modifications. Recently, laser texturing has been successfully applied for different materials, included NiTi shape memory alloys, and also for tuning the surface properties, such as wettability. In the present work, ultrashort laser surface modification was carried out, through the use of a femtosecond laser, for the surface texturing of commercial superelastic NiTi plates. The main goal is to investigate the correlation among morphology, chemical composition and wettability with the principal process parameters, such as average power and scanning velocity in high power ultrashort laser texturing. Laser patterned surfaces were characterized by means of scanning electron microscopy, 3D-profilometry, XPS analysis and wetting measurements. After the laser treatments, both surface morphology and Ni/Ti ratio were largely modified from the initial surface, depending on the adopted process parameters. The wettability of the laser textured surfaces can be also varied with respect to the initial surface, due to the roughness values and grooves induced by the laser beam scans. The laser texturing process induced a combination between micro and nano structures, depending on the input energy. In details, the surfaces were tuned to lower roughness values (from 0.4 μm to 0.3 μm) with a laser power of 1 W, while it was increased up to 0.65 μm with a laser power of 13 W. The laser surface modification promoted a change of the contact angle from 70° of the untreated condition up to 135° to the surface laser treated with a power of 13 W.
Development of a Non-Integral Form of Coordination Number Equation Based on Pair Distribution Function and Gaussian Function
Authors: Wang Chunlong , Chen Xiumin , Tao Dongping
Published: February 2023

The coordination number (CN) is an important structure property of liquid metals. A simple yet extremely precise method for calculating CN is proposed, the classical CN methods are evaluated systematically, and the mathematical forms of the symmetry method are corrected. Using the Gaussian function construct, the first coordination shell of the pair distribution function (PDF), the right-hand side of the first peak of the pair distribution function is extrapolated, and the CN expression is simplified with a Gaussian function to obtain its non-integral form. The first coordination shell is used to explain the Tao coordination number model (Tao CN) and obtain a Modified Tao CN. The Gaussian function is combined with the Tao CN, obtaining the function expression for the peak with peak position. These are important for the structural research of liquid metals. The CN of 27 liquid metals is calculated by these methods. The average relative deviation of the Gaussian function extrapolation method is ±6.46%, of the Modified Tao CN is ± 18.51%; those of the four classical methods range from ±15% to ±42%. The Modified Tao CN and extrapolation methods to calculate CN are more accurate for calculating CN than the classical method; they are more suitable for use in quantitative applications of CN. The equations derived in this work can be applied to the problem of integration of distribution functions to obtain simple mathematical models.