bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Aging Effect of Ester Liquids and Mineral Oil in Semi-Uniform Field Geometry under Lightning Impulse Voltage and Standard Compliant AC Voltage Testing
Authors: Krügel Sabrina , Plath Ronald
Published: October 2024

This study examines and compares the breakdown and aging properties of five insulating liquids. Additionally, the influence of different voltage polarities on these properties was analyzed to investigate the effect of aging on polarity behavior under lightning impulse voltage in a semi-uniform field. The results were compared to standardized AC breakdown tests. After 2330 h and 4350 h of aging, changes were observed in key aging indicators such as water content (both absolute and relative), total acid number, and color across all liquids. Viscosity increased by up to 10% in natural esters. Notably, the rise in water content due to aging was concerning only for mineral oil, exceeding 20%. The impact of aging on breakdown voltage varied depending on the voltage type and polarity. Aging had the least effect under negative lightning impulse voltage, while the synthetic ester MIDEL 7131 exhibited the most significant reduction in breakdown voltage under positive lightning impulse voltage, dropping by over 24%, from more than 560 kV to 428 kV. In contrast, mineral oil showed only a 3% decrease. For the other liquids, the most pronounced reduction in breakdown voltage due to aging occurred under AC voltage, with natural esters showing a 17% decline, synthetic esters 26%, and mineral oil experiencing a 38% reduction.
An Experimental Comparative Study of Large-Sized Direct Solar Fryers for Injera Baking Applications
Authors: Hailu Mesele Hayelom , Kahsay Mulu Bayray , Tesfay Asfafaw Haileslassie , Nydal Ole Jørgen
Published: October 2024

This research experimentally demonstrates the practicability of using large-sized direct solar frying as an alternative technology for the predominantly biomass-based injera baking method. The system was designed and developed with fryers 40, 50, and 55 cm in diameter and two operational options: continuous mode and alternating mode. Extensive experimental testing was conducted on each prototype to demonstrate solar frying and determine the relative performance. The findings indicate that the 2 kW heating capacity of the 40 cm-sized solar fryer model conducted baking processes at a relatively lower system temperature in both application modes compared to the larger-sized fryers. As a result, this system maintained a consistent average fryer temperature distribution and shorter initial heating time, without the requirement of a reheating process during the subsequent baking cycles. The experimental testing also demonstrated that alternating-mode applications were more practical for the 40 cm-sized fryers than for the larger ones. Overall, direct solar frying is more efficient and convenient for the 40 cm-sized solar fryers. In contrast, the larger-sized systems required a larger fryer thermal storage capacity coupled with larger-size solar concentrators to maintain equivalent stable operational conditions, conversely leading to a lack of application simplicity and higher system costs.
Control System for the Performance Analysis of Turbines at Laboratory Scale
Authors: Obando Vega Felipe , Rubio-Clemente Ainhoa , Chica Edwin
Published: October 2024

The generation of sustainable energy through wind and hydrokinetic turbines, which convert the kinetic energy from fluid flows into mechanical energy, presents an attractive solution for diversifying the country energy matrix in response to climate change. Consequently, numerous studies have investigated the aerodynamic and hydrodynamic behaviors of various wind and hydrokinetic turbines using numerical simulations to understand their interaction with the surrounding fluid flows and enhance their performance. However, to validate these studies and aiming at improving the turbine design, experimental studies on a laboratory scale employing wind tunnels and hydraulic channels are essential. This work addresses the development and implementation of a reliable control system for experimentally evaluating the power coefficient (Cp) versus the tip speed ratio (TSR) curve of wind and hydrokinetic turbines. The control system, based on a DC motor acting as a generator and aligned with a commercial torque sensor, enables a precise control over the experimental setup. By obtaining and comparing the experimental performance curves of Cp versus TSR for both wind and hydrokinetic turbines with numerical results, the effectiveness and accuracy of the developed control system are demonstrated. A satisfactory fit between numerical and experimental results was achieved, underscoring the utility and reliability of the control system for assessing the turbine performance.
Cement-Formation Debonding Due to Temperature Variation in Geothermal Wells: An Intensive Numerical Simulation Assessment
Authors: Lambrescu Ionut , Abid Khizar , Teodoriu Catalin
Published: October 2024

Geothermal wells are subjected to higher loads compared to conventional oil and gas wells due to the thermal cycles that occur during both production and non-production phases. These temperature variations can affect the cohesion of the cement within the formation and casing, creating micro-annuli channels that can ultimately compromise the integrity of the well. Therefore, this study employs an intensive finite element methodology to analyze the debonding criteria of casing–cement systems in geothermal wells by examining over 36 independent models. The wellbore cooling and heating processes were simulated using three cohesive zone models (CZM): Type I (tensile), Type II (shear), and mixed (Type I and II simultaneously). The analysis revealed that Type I debonding occurs first during cooling at a temperature of around 10 °C, while Type II is the primary failure mode during heating. Evaluations of interfacial bonding shear strength (IBSS) values indicated that the debonding of the cement would even occur at high IBSS values (3 and 4 MPa) at a differential temperature of 300 °C, while the other IBSS of 1 MPa withstands only 60 °C. However, achieving an IBSS of 4 MPa with current technology is highly unlikely. Therefore, geothermal well operation and construction must be modified to keep the differential temperature below the critical temperature at which the debonding of the cement initiates. The study also found that debonding during cooling happens at lower differential temperatures due to generally lower values for interfacial bonding tensile strength (IBTS), typically less than 1 MPa. The novelty of the study is that it provides new insights into how specific temperatures trigger different types of debonding, highlights that high IBSS values may not prevent debonding at high differential temperatures, and recommends operational adjustments to maintain temperatures below critical levels to enhance cement integrity. Additionally, this study reveals that debonding during cooling occurs at a lower differential temperature change due to the reduced value of the interfacial bonding tensile strength (IBTS).
Stakeholders’ Perceptions of the Peer-to-Peer Energy Trading Model Using Blockchain Technology in Indonesia
Authors: Yusuf Faisal , Sari Riri , Yusgiantoro Purnomo , Soesilo Tri
Published: October 2024

The energy transition toward Net Zero Emission by 2060 hinges on the renewable energy power plants in Indonesia. Good practices in several countries suggest a peer-to-peer (P2P) energy trading system using blockchain technology, supported by renewable energy (solar panels), an innovation to provide equal access to sustainable electricity while reducing the impact of climate change. The P2P energy trading concept has a higher social potential than the conventional electricity buying and selling approach, such as that of PLN (the state-owned electricity company in Indonesia), which applies the network management concept but does not have a sharing element. This model implements a solar-powered mini-grid system and produces a smart contract that facilitates electricity network users to buy, sell, and trade electricity in rural areas via smartphones. This study aims to measure the stakeholders’ perceptions of the peer-to-peer (P2P) energy trading model using blockchain technology in the Gumelar District, Banyumas Regency, Central Java Province, Indonesia. The stakeholders in question are representatives of Households (producers and consumers), Government, State Electricity Company (PLN), Non-Governmental Organizations, Private Sector and Academician. Measurement of perception in this study used a questionnaire approach with a Likert scale. The results of filling out the questionnaire were analyzed using four methods: IFE/EFE matrix; IE matrix; SWOT matrix; and SPACE matrix to assess the results and their suitability to each other. The results of the stakeholder perception assessment show that there are 44 internal factors and 33 external factors that can influence this model. We obtained an IFE and EFE score of 2.92 and 2.83 for the internal and external results using the IE matrix. These place the model in quadrant V, meaning the P2P model can survive in the long term to generate profits. Based on the SWOT analysis results, this model is located at the coordinate point −0.40, 0.31, placing it in quadrant II. This means that the P2P model is in a competitive situation and faces threats but still has internal strengths. Based on the SPACE matrix, stakeholder perception states that the P2P model is at coordinate point 1, −0.3. This shows that the P2P model has the potential to be a competitive advantage in its type of activity that continues to grow. In conclusion, our findings show that stakeholders’ perceptions of P2P models using blockchain technology can be implemented effectively and provide social, economic, and environmental incentives.
Altitudinal Influences on Soil Microbial Diversity and Community Assembly in Topsoil and Subsoil Layers: Insights from the Jinsha River Basin, Southwest China
Authors: Guo Zhihong , Huang Xiaobo , Wang Tongli , Su Jianrong , Li Shuaifeng
Published: October 2024

Mountain regions play a crucial role in maintaining global biodiversity, with altitude exerting a significant influence on soil microbial diversity by altering plant diversity, soil nutrients, and microclimate. However, differences in microbial community composition between topsoil (0–10 cm deep) and subsoil (10–20 cm deep) remain poorly understood. Here, we aimed to assess soil microbial diversity, microbial network complexity, and microbial community assembly in the topsoil and subsoil layers of the dry–hot Jinsha River valley in southwestern China. Using high-throughput sequencing in soil samples collected along an altitudinal gradient, we found that bacterial diversity in topsoil decreased with increasing altitude, while bacterial diversity in subsoil showed no altitude-dependent changes. Fungal diversity in topsoil also varied with altitude, while subsoil fungal diversity showed no change. These findings suggest that microbial diversity in topsoil was more sensitive to changes in altitude than subsoil. Bacterial community assembly tended to be governed by stochastic processes, while fungal assembly was deterministic. Soil bacterial and fungal network complexity was enhanced with increasing altitude but reduced as diversity increased. Interestingly, the presence of woody plant species negatively affected bacterial and fungal community composition in both soil layers. Soil pH and water content also negatively affected microbial community composition, while organic carbon and total nitrogen positively influenced the microbial community composition. Simultaneously, herb and woody plant diversity mainly affected soil bacterial diversity in the topsoil and subsoil, respectively, while woody plant diversity mainly affected soil fungal diversity in subsoil and soil nutrients had more effect on soil fungal diversity. These findings suggest that altitude directly and indirectly affects microbial diversity in topsoil, subsequently influencing microbial diversity in subsoil through nutrient availability.
Assessment of New Techniques for Measuring Volume in Large Wood Chip Piles
Authors: Gejdoš Miloš , Výbošťok Jozef , Chudá Juliána , Tomčík Daniel , Lieskovský Martin , Skladan Michal , Masný Matej , Gergeľ Tomáš
Published: October 2024

Our work aimed to compare the chip pile volumes calculated by laser ground scanning, UAV technology, and laser ground measurement and also to determine the accuracy, speed, and economic efficiency of each method. The large chip pile was measured in seven different ways: band measurement, laser measurement with Vertex, global navigation satellite system, handheld mobile laser scanner, terrestrial laser scanner, drone, and smartphone with a light detection and ranging sensor. All the methods were compared in terms of accuracy, price, user-friendliness, and time required to obtain results. The calculated pile volume, depending on the method, varied from 2588 to 3362 m3. The most accurate results were provided by the terrestrial laser scanning method, which, however, was the most expensive and the most demanding in terms of collecting and evaluating the results. From a time and economic point of view, the most effective methods were UAVs and smartphones with LiDAR.
Study on Nondestructive Detection Imaging Method of Log Knot Based on Judging the Shortest Path of Stress Wave Propagation
Authors: Liu Fenglu , Wang Qinhui , Wu Chuanyu , Chen Wenhao , Xiao Jiawei
Published: October 2024

This paper presents an iterative stress wave log beam imaging algorithm based on external shape to predict the size, shape, and position of internal features in logs. The algorithm’s accuracy is analyzed to establish a theoretical and scientific basis for the prediction and evaluation of internal knots in standing trees. Six sample logs with natural knots were selected for study, and cross-sectional stress wave propagation tests were conducted using FAKOPP to collect data. Using the shortest propagation path method, the algorithm iteratively produced fault images of the log cross-sections. While the algorithm can roughly predict the location of internal features, discrepancies between predicted and actual shapes and sizes result in relative errors ranging from 15.66% to 52.08%. Except for sample log 6, the relative error for other logs is mostly within 31%, with logs 1, 2, and 5 showing errors under 20%. However, the imaging accuracy and effectiveness need improvement. Further experimental studies and algorithm enhancements are necessary to improve fault imaging and prediction accuracy, particularly in terms of shape and size precision.
Analysis of Mpox by Occupation and Industry in Seven U.S. Jurisdictions, May 2022–March 2023
Authors: Groenewold Matthew R. , de Perio Marie A. , Moller Kyle M. , Bui David , Saadeh Kayla , Still William , Meh Ie , Lavender Antionette , Soliva Susan , Fields Caitlin , Hopkins Brandi , Laramie Angela K. , Harrington Pauline , Stout Allen , Levenson Cheri , Morris Collin R. , Creswell Paul D. , Segaloff Hannah E. , Somerville Nicholas J. , Dowell Chad H. , Delaney Lisa J.
Published: October 2024

During responses to outbreaks, the collection and analysis of data on employed case patients’ industry and occupation are necessary to better understand the relationship between work and health outcomes. The occurrence of mpox by occupation and industry has not previously been assessed in the context of the 2022 outbreak. We analyzed employment data from 2548 mpox cases reported to the U.S. Centers for Disease Control and Prevention from surveillance systems in seven U.S. jurisdictions and population-based reference data on employment patterns from the U.S. Bureau of Labor Statistics to describe the differential proportionate distribution of cases across occupation and industry groups using the proportionate morbidity ratio. In gender-specific analyses, we found that men employed in certain occupations and industries had a higher relative risk of mpox than others. While occupational transmission cannot be ruled out, it is more likely that individuals with personal and behavioral risk factors for mpox were more likely to work in these occupations and industries. This analysis provides an example of collecting and analyzing occupation and industry data in case reports to understand possible differences in risk by occupation and industry in infectious disease outbreak investigation and help inform resource allocation, messaging, and response.
Evaluation of Retinal Structure and Visual Function in Blue Cone Monochromacy to Develop Clinical Endpoints for L-opsin Gene Therapy
Authors: Cideciyan Artur V. , Roman Alejandro J. , Warner Raymond L. , Sumaroka Alexander , Wu Vivian , Jiang Yu Y. , Swider Malgorzata , Garafalo Alexandra V. , Viarbitskaya Iryna , Russell Robert C. , Kohl Susanne , Wissinger Bernd , Ripamonti Caterina , Barbur John L. , Bach Michael , Carroll Joseph , Morgan Jessica I. W. , Aleman Tomas S.
Published: October 2024

L-cone opsin expression by gene therapy is a promising treatment for blue cone monochromacy (BCM) caused by congenital lack of long- and middle-wavelength-sensitive (L/M) cone function. Eight patients with BCM and confirmed pathogenic variants at the OPN1LW/OPN1MW gene cluster participated. Optical coherence tomography (OCT), chromatic perimetry, chromatic microperimetry, chromatic visual acuity (VA), and chromaticity thresholds were performed with unmodified commercial equipment and/or methods available in the public domain. Adaptive optics scanning laser ophthalmoscope (AOSLO) imaging was performed in a subset of patients. Outer retinal changes were detectable by OCT with an age-related effect on the foveal disease stage. Rod and short-wavelength-sensitive (S) cone functions were relatively retained by perimetry, although likely impacted by age-related increases in the pre-retinal absorption of short-wavelength lights. The central macula showed a large loss of red sensitivity on dark-adapted microperimetry. Chromatic VAs with high-contrast red gratings on a blue background were not detectable. Color vision was severely deficient. AOSLO imaging showed reduced total cone density with majority of the population being non-waveguiding. This study developed and evaluated specialized outcomes that will be needed for the determination of efficacy and safety in human clinical trials. Dark-adapted microperimetry with a red stimulus sampling the central macula would be a key endpoint to evaluate the light sensitivity improvements. VA changes specific to L-opsin can be measured with red gratings on a bright blue background and should also be considered as outcome measures in future interventional trials.
The Immune Modulation HLA-G*01:01:01 Full Allele Is Associated with Gastric Adenocarcinoma Development
Authors: Suarez-Trujillo Fabio , Juarez Ignacio , Vaquero-Yuste Christian , Gutierrez-Calvo Alberto , Lopez-García Adela , Lasa Inmaculada , Gomez Remedios , Martin-Villa José Manuel , Arnaiz-Villena Antonio
Published: October 2024

The Human Leukocyte Antigen (HLA) system contains a set of genes involved at many levels in the innate and adaptive immune response. Among the non-classical HLA class I genes, HLA-G stands out for the numerous studies about its pivotal role in regulating/modulating immune responses. Also, its involvement in extravillous cytotrophoblast function, viral infections, autoimmunity, and cancer has been extensively documented. The present study explores for the first time the relationship between natural alleles of HLA-G, rather than STSs, SNPs, or partial gene polymorphisms, and the development of gastric adenocarcinoma, by analyzing the genetic profile of a cohort of 40 Spanish patients with this type of tumor using DNA extracted from paired biopsies of tumoral and adjacent non-tumoral gastric tissue. Our results reveal a significant statistical relationship between the presence of the HLA-G*01:01:01 allele and the development of gastric cancer, while other common alleles such as -G*01:04 or -G*01:05N did not demonstrate a significant correlation. Studying the involvement of HLA genes in the development of many diseases is relevant to understanding their pathophysiology. However, the absence of specific mechanisms underlying these associations suggests that investigating complete HLA natural alleles’ extended haplotypes or complotypes may offer a more precise and valuable approach to elucidating the association of HLA with the pathogenesis of disease.
New Small-Molecule SERCA Inhibitors Enhance Treatment Efficacy in Lenvatinib-Resistant Papillary Thyroid Cancer
Authors: Kim Jungmin , Chang Hang-Seok , Yun Hyeok Jun , Chang Ho-Jin , Park Ki Cheong
Published: October 2024

Papillary thyroid cancer (PTC) is one of the most treatable forms of cancer, with many cases being fully curable. However, resistance to anticancer drugs often leads to metastasis or recurrence, contributing to the failure of cancer therapy and, ultimately, patient mortality. The mechanisms underlying molecular differences in patients with metastatic or recurrent PTC, particularly those resistant to anticancer drugs through epigenetic reprogramming, remain poorly understood. Consequently, refractory PTC presents a critical challenge, and effective therapeutic strategies are urgently needed. Therefore, this study aimed to identify small-molecule inhibitors to enhance treatment efficacy in lenvatinib-resistant PTC. We observed an increase in sarco/endoplasmic reticulum calcium ATPase (SERCA) levels in patient-derived lenvatinib-resistant PTC cells compared with lenvatinib-sensitive ones, highlighting its potential as a therapeutic target. We subsequently identified two SERCA inhibitors [candidates 40 (isoflurane) and 42 (ethacrynic acid)] through in silico screening. These candidates demonstrated significant tumor shrinkage in a xenograft tumor model and reduced cell viability in patient-derived lenvatinib-resistant PTC cells when used in combination with lenvatinib. Our findings have potential clinical value for the development of new combination therapies to effectively target highly malignant, anticancer drug-resistant cancers.
Perry Disease: Current Outlook and Advances in Drug Discovery Approach to Symptomatic Treatment
Authors: Gajda Zbigniew , Hawrylak Magdalena , Handzlik Jadwiga , Kuder Kamil J.
Published: October 2024

Perry disease (PeD) is a rare, neurodegenerative, genetic disorder inherited in an autosomal dominant manner. The disease manifests as parkinsonism, with psychiatric symptoms on top, such as depression or sleep disorders, accompanied by unexpected weight loss, central hypoventilation, and aggregation of DNA-binding protein (TDP-43) in the brain. Due to the genetic cause, no causal treatment for PeD is currently available. The only way to improve the quality of life of patients is through symptomatic therapy. This work aims to review the latest data on potential PeD treatment, specifically from the medicinal chemistry and computer-aided drug design (CADD) points of view. We select proteins that might represent therapeutic targets for symptomatic treatment of the disease: monoamine oxidase B (MAO-B), serotonin transporter (SERT), dopamine D2 (D2R), and serotonin 5-HT1A (5-HT1AR) receptors. We report on compounds that may be potential hits to develop symptomatic therapies for PeD and related neurodegenerative diseases and relieve its symptoms. We use Phase pharmacophore modeling software (version 2023.08) implemented in Schrödinger Maestro as a ligand selection tool. For each of the chosen targets, based on the resolved protein–ligand structures deposited in the Protein Data Bank (PDB) database, pharmacophore models are proposed. We review novel, active compounds that might serve as either hits for further optimization or candidates for further phases of studies, leading to potential use in the treatment of PeD.
Higher Steroid Production in the Right Adrenal Gland Compared to the Left One in db/db Mice, a Model of Type 2 Diabetic Obesity
Authors: Saxu Rengui , Luo Qiming , Yang Yong , Gu Harvest F.
Published: October 2024

Vertebrates exhibit a left–right asymmetry from the central structures to the peripheral paired endocrine organs. However, the asymmetries in paired endocrine glands and the pathological consequences of such asymmetries remain largely unknown. The adrenal gland constitutes a pair of peripheral end organs in the neuroendocrine system, responsible for producing steroid hormones under stimuli. In the present study, the lateralized asymmetry of left and right adrenal glands in leptin receptor-deficit db/db mice was investigated. First, a morphological and histological examination showed that adrenal mass and adrenal cortex volume in db/db mice were significantly higher than in non-diabetic control mice. Then, adrenal transcriptomic and serum metabolomic analyses were performed. Adrenal steroid profiling showed that the levels of corticosterone and aldosterone in the right adrenal gland of db/db mice were two times higher than in the left one. The expression of multiple genes related to adrenal regeneration and innervation in db/db mice was reduced in contrast to the increased steroid hormone secretion. Furthermore, an examination of morphogens in asymmetric adrenal development revealed a significant differential expression of Shh and its receptor gene Ptch1. In conclusion, the present study has provided evidence that a superior steroidogenesis exists in the right adrenal gland of db/db mice and suggested that Shh signaling may play an important role in asymmetric adrenal responses in type 2 diabetes and its complications.
Trehalose Attenuates In Vitro Neurotoxicity of 6-Hydroxydopamine by Reducing Oxidative Stress and Activation of MAPK/AMPK Signaling Pathways
Authors: Stevanovic Danijela , Vucicevic Ljubica , Misirkic-Marjanovic Maja , Martinovic Tamara , Mandic Milos , Harhaji-Trajkovic Ljubica , Trajkovic Vladimir
Published: October 2024

The effects of trehalose, an autophagy-inducing disaccharide with neuroprotective properties, on the neurotoxicity of parkinsonian mimetics 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenylpiridinium (MPP+) are poorly understood. In our study, trehalose suppressed 6-OHDA-induced caspase-3/PARP1 cleavage (detected by immunoblotting), apoptotic DNA fragmentation/phosphatidylserine externalization, oxidative stress, mitochondrial depolarization (flow cytometry), and mitochondrial damage (electron microscopy) in SH-SY5Y neuroblastoma cells. The protection was not mediated by autophagy, autophagic receptor p62, or antioxidant enzymes superoxide dismutase and catalase. Trehalose suppressed 6-OHDA-induced activation of c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and AMP-activated protein kinase (AMPK), as revealed by immunoblotting. Pharmacological/genetic inhibition of JNK, p38 MAPK, or AMPK mimicked the trehalose-mediated cytoprotection. Trehalose did not affect the extracellular signal-regulated kinase (ERK) and mechanistic target of rapamycin complex 1 (mTORC1)/4EBP1 pathways, while it reduced the prosurvival mTORC2/AKT signaling. Finally, trehalose enhanced oxidative stress, mitochondrial damage, and apoptosis without decreasing JNK, p38 MAPK, AMPK, or AKT activation in SH-SY5Y cells exposed to MPP+. In conclusion, trehalose protects SH-SY5Y cells from 6-OHDA-induced oxidative stress, mitochondrial damage, and apoptosis through autophagy/p62-independent inhibition of JNK, p38 MAPK, and AMPK. The opposite effects of trehalose on the neurotoxicity of 6-OHDA and MPP+ suggest caution in its potential development as a neuroprotective agent.
Immunostimulatory Activity of a Mixture of Platycodon grandiflorum, Pyrus serotine, Chaenomeles sinensis, and Raphanus sativus in RAW264.7 Macrophages
Authors: Rod-in Weerawan , Kim Minji , Jang A-yeong , Nam Yu Suk , Yoo Tae Young , Park Woo Jung
Published: October 2024

In this study, a mixture of Platycodon grandiflorum, Pyrus serotina, Chaenomeles sinensis, and Raphanus sativus (PPCRE) was investigated for their immuno-enhancing effects, as well as the molecular mechanism of PPCRE in RAW264.7 cells. PPCRE dramatically increased nitric oxide (NO) and prostaglandin E2 (PGE2) generation depending on the concentration while exhibiting no cytotoxicity. PPCRE markedly upregulated the mRNA and protein expression of immune-related cytotoxic factors such as cyclooxygenase (COX)-1, COX-2, and inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha (TNF-α), as well as the mRNA level of IL-4. PPCRE increased the mitogen-activated protein kinase (MAPK) signaling pathway by upregulating the phosphorylation of extracellular signal-regulated kinase (ERK), stress-activated protein kinase/Jun N-terminal-kinase (SAPK/JNK), and p38. Furthermore, PPCRE considerably activated the nuclear factor kappa B (NF-κB) signaling pathway by increasing phosphorylation of NF-κB-p65. PPCRE-stimulated RAW264.7 cells increased macrophage phagocytic capacity. In conclusion, our study found that PPCRE improved immune function by modulating inflammatory mediators and regulating the MAPK and NF-κB pathway of signaling in macrophages.
Potential Teratogenicity Effects of Metals on Avian Embryos
Authors: Szabó Rita , Budai Péter , Juhász Éva , Major László , Lehel József
Published: October 2024

Agricultural areas can provide sources of food and hiding and nesting places for wild birds. Thus, the chemical load of potentially toxic elements (Cd, Cu, Pb) due to industrial and agricultural activities can affect not only the adult birds but also the embryos developing in the egg. The toxic effects of heavy metals applied alone were investigated on chicken embryos in the early and late stages of embryonic development using injection and immersion treatment methods. On day 3 of incubation, permanent preparations were made from the embryos to study the early development stage. There were no significant differences observed in embryo deaths and developmental abnormalities in this stage. On day 19 of incubation, the number of embryonic deaths, the body weight of the embryos, and the type of developmental abnormalities were examined. The embryonic mortality was statistically higher in the groups treated with cadmium and lead in the case of the injection treatment. A significant increase in developmental disorders was observed in the copper-treated group using the immersion application. The body weight significantly decreased in the cadmium- and lead-treated group using both treatment methods. However, a significant change in the body weight in the copper-treated group was only realized due to the injection method.
Histopathological and Immunohistochemical Characteristics of Different Types of Cardiac Amyloidosis
Authors: Gioeva Zarina V. , Mikhaleva Liudmila M. , Gutyrchik Nikita A. , Volkov Alexey V. , Popov Mikhail A. , Shakhpazyan Nikolay K. , Pechnikova Valentina V. , Midiber Konstantin Y. , Reznik Elena V. , Kakturskij Lev V.
Published: October 2024

Cardiac involvement is the most important factor determining prognosis in patients with systemic amyloidosis. This retrospective observational study of 98 patients with amyloidosis was undertaken to assess the amyloid types that are most likely to affect the heart, describe histopathological and clinical features of cardiac amyloidosis, and estimate the number of cases not diagnosed clinically prior to death. All cases were divided into two groups based on the method of examination. The first group included 46 patients with cardiac amyloidosis revealed via endomyocardial biopsies (EMBs), and the second group included 52 amyloidosis patients who did not undergo EMBs, in whom cardiac involvement was identified only at autopsy. The EMBs demonstrated that AL amyloidosis was detected in 21 (46%) specimens, ATTR amyloid in 24 cases (52%), and AA amyloid in 1 case (2%). The autopsy reports defined 15 (46%) cases of AL amyloidosis, 21 (40%) of ATTR and 16 (31%) of AA amyloidosis. It should be noted that a clinical diagnosis of ATTR amyloidosis was made only in 9.5% of patients from the autopsy group, suggesting that ATTR may be an underdiagnosed cause of heart failure in elderly patients. The most intense amyloid deposits were determined in biopsy and autopsy specimens of patients with AL kappa amyloidosis, underlying a poorer prognosis.
C5a Induces Inflammatory Signaling and Apoptosis in PC12 Cells through C5aR-Dependent Signaling: A Potential Mechanism for Adrenal Damage in Sepsis
Authors: Mrozewski Lucas , Tharmalingam Sujeenthar , Michael Paul , Kumar Aseem , Tai T. C.
Published: October 2024

The complement system is critically involved in the pathogenesis of sepsis. In particular, complement anaphylatoxin C5a is generated in excess during sepsis, leading to cellular dysfunction. Recent studies have shown that excessive C5a impairs adrenomedullary catecholamine production release and induces apoptosis in adrenomedullary cells. Currently, the mechanisms by which C5a impacts adrenal cell function are poorly understood. The PC12 cell model was used to examine the cellular effects following treatment with recombinant rat C5a. The levels of caspase activation and cell death, protein kinase signaling pathway activation, and changes in inflammatory protein expression were examined following treatment with C5a. There was an increase in apoptosis of PC12 cells following treatment with high-dose C5a. Ten inflammatory proteins, primarily involved in apoptosis, cell survival, and cell proliferation, were upregulated following treatment with high-dose C5a. Five inflammatory proteins, involved primarily in chemotaxis and anti-inflammatory functions, were downregulated. The ERK/MAPK, p38/MAPK, JNK/MAPK, and AKT protein kinase signaling pathways were upregulated in a C5aR-dependent manner. These results demonstrate an apoptotic effect and cellular signaling effect of high-dose C5a. Taken together, the overall data suggest that high levels of C5a may play a role in C5aR-dependent apoptosis of adrenal medullary cells in sepsis.
Effects of PACAP Deficiency on Immune Dysfunction and Peyer’s Patch Integrity in Adult Mice
Authors: Sparks Jason , Meggyes Matyas , Makszin Lilla , Jehn Viktoria , Lugosi Hedvig , Reglodi Dora , Szereday Laszlo
Published: October 2024

PACAP (pituitary adenylate cyclase activating polypeptide) is a widespread neuropeptide with cytoprotective and anti-inflammatory effects. It plays a role in innate and adaptive immunity, but data are limited about gut-associated lymphoid tissue. We aimed to reveal differences in Peyer’s patches between wild-type (WT) and PACAP-deficient (KO) mice. Peyer’s patch morphology from young (3-months-old) and aging (12–15-months-old) mice was examined, along with flow cytometry to assess immune cell populations, expression of checkpoint molecules (PD-1, PD-L1, TIM-3, Gal-9) and functional markers (CD69, granzyme B, perforin) in CD3+, CD4+, and CD8+ T cells. We found slight differences between aging, but not in young, WT, and KO mice. In WT mice, aging reduced CD8+ T cell numbers frequency and altered checkpoint molecule expression (higher TIM-3, granzyme B; lower Gal-9, CD69). CD4+ T cell frequency was higher with similar checkpoint alterations, indicating a regulatory shift. In PACAP KO mice, aging did not change cell population frequencies but led to higher TIM-3, granzyme B and lower PD-1, PD-L1, Gal-9, and CD69 expression in CD4+ and CD8+ T cells, with reduced overall T cell activity. Thus, PACAP deficiency impacts immune dysfunction by altering checkpoint molecules and T cell functionality, particularly in CD8+ T cells, suggesting complex immune responses by PACAP, highlighting its role in intestinal homeostasis and potential implications for inflammatory bowel diseases.
Exploring the Impact of Extracorporeal Membrane Oxygenation on the Endothelium: A Systematic Review
Authors: Li Yakun , Volleman Carolien , Dubelaar Dionne P. C. , Vlaar Alexander P. J. , van den Brom Charissa E.
Published: October 2024

Extracorporeal membrane oxygenation (ECMO) is a life-saving intervention for patients with circulatory and/or pulmonary failure; however, the rate of complications remains high. ECMO induces systemic inflammation, which may activate and damage the endothelium, thereby causing edema and organ dysfunction. Advancing our understanding in this area is crucial for improving patient outcomes during ECMO. The goal of this review is to summarize the current evidence of the effects of ECMO on endothelial activation and damage in both animals and patients. PubMed and Embase databases were systematically searched for both clinical and animal studies including ECMO support. The outcome parameters were markers of endothelial activation and damage or (in)direct measurements of endothelial permeability, fluid leakage and edema. In total, 26 studies (patient n = 16, animal n = 10) fulfilled all eligibility criteria, and used VA-ECMO (n = 13) or VV-ECMO (n = 6), or remained undefined (n = 7). The most frequently studied endothelial activation markers were adhesion molecules (ICAM-1) and selectins (E- and P-selectin). The levels of endothelial activation markers were comparable to or higher than in healthy controls. Compared to pre-ECMO or non-ECMO, the majority of studies showed stable or decreased levels. Angiopoietin-2, von Willebrand Factor and extracellular vesicles were the most widely studied circulating markers of endothelial damage. More than half of the included studies showed increased levels when compared to normal ranges, and pre-ECMO or non-ECMO values. In healthy animals, ECMO itself leads to vascular leakage and edema. The effect of ECMO support in critically ill animals showed contradicting results. ECMO support (further) induces endothelial damage, but endothelial activation does not, in the critically ill. Further research is necessary to conclude on the effect of the underlying comorbidity and type of ECMO support applied on endothelial dysfunction.
Comparison of a Novel Liquid Embolic System with Commonly Used Embolic Agents in the Endovascular Treatment of Intracranial Dural Arteriovenous Fistulas: A Single-Center Experience
Authors: Nedeljkovic Zarko , Vukasinovic Ivan , Petrovic Masa , Nedeljkovic Aleksandra , Nastasovic Tijana , Bascarevic Vladimir , Micovic Mirko , Milicevic Mihailo , Milic Marina , Jovanovic Nemanja , Stanimirovic Aleksandar , Scepanovic Vuk , Grujicic Danica
Published: October 2024

Background/Objectives: Endovascular embolization is an effective treatment option for cerebral arteriovenous malformation (AVM) and dural arteriovenous fistulas (DAVFs). The objective of this study was to assess the safety and efficacy of MenoxTM in patients with cranial dural arteriovenous fistulas. Methods: From January 2021 to January 2023, 19 patients with intracranial DAVFs underwent embolization procedures. All patients were treated by embolization with MenoxTM or/and in combination with other embolization products such as Onyx (Covidien, Irvine, California), PHIL (MicroVention, Tustin, California), and Squid (Balt Extrusion, Montmorency, France). Treatment approaches were selected depending on the anatomical location of the fistula. Patients were monitored and followed-up for 12 months. Results: The patients’ mean age was 56.26 ± 16.49 years. Of these 19 patients, 58% (n = 11) were treated with the MenoxTM liquid embolizing agent (LEA) alone or in combination with different LEAs, while n = 7 were treated with other LEAs and 1 patient was treated solely with coils. Complete occlusion of DAVFs with MenoxTM and other agents was evident in 68.4% (n = 13/19) of patients. Complete occlusion (100%) was observed in the sinus rectus, transverse sinus, and diploic veins of the orbital roof, while complete occlusion was observed in 50% of falcotentorial patients and 60% of superior sagittal sinus patients. The lowest rate of complete fistula obliteration was observed in the dural carotid cavernous fistula (CCF) group (25%). An intra-procedural adverse event occurred in one patient. No other post-procedural adverse events were noted. Furthermore, in patients treated with MenoxTM, total occlusion was achieved in 72.7% (n = 8) of patients, whereas the non-MenoxTM group had 62.5% (n = 5) of patients with 100% occlusion and 37.5% (n = 3) of patients with subtotal occlusion. Conclusions: Outcomes using MenoxTM alone and in combination with other agents were effective, and it is safe for the treatment of dural arteriovenous fistulas.
The Association between Suicidal Ideation and Subtypes of Comorbid Insomnia Disorder in Apneic Individuals
Authors: Hein Matthieu , Wacquier Benjamin , Conenna Matteo , Lanquart Jean-Pol , Point Camille
Published: October 2024

Background/Objectives: Given the existence of higher suicidality in apneic individuals, this study aimed to determine the potential role played by subtypes of the comorbid insomnia disorder (CID) in the occurrence of suicidal ideation for this specific subpopulation. Methods: To perform our analyses, 1488 apneic individuals were retrospectively extracted from the Sleep Laboratory hospitalization register. Only apneic individuals with suicidal ideation highlighted during the psychiatric interview and/or with a score ≥1 on item G of the Beck Depression Inventory confirmed during the clinical interview were included in the group with suicidal ideation. The likelihood of suicidal ideation associated with CID subtypes was investigated using logistic regression analyses. Results: The prevalence of suicidal ideation was 9.3% in our sample of apneic individuals. After hierarchically introducing the significant confounders for adjustment, multivariate logistic regression analyses demonstrated that unlike short sleep duration alone and CID without short sleep duration, the likelihood of suicidal ideation was only higher for CID with short sleep duration in apneic individuals. Conclusions: Thus, we highlighted in this study that CID with short sleep duration could play a major role in higher suicidality for apneic individuals, which seems to require systematic screening and appropriate treatment of this comorbid sleep disorder to enable better management of suicidal risk in this specific subpopulation.
Effect of Spray Characteristic Parameters on Friction Coefficient of Ultra-High-Strength Steel against Cemented Carbide
Authors: Wu Bangfu , Zhang Minxiu , Zhao Biao , Li Benkai , Ding Wenfeng
Published: October 2024

Ultra-high-strength steels have been considered an essential material for aviation components owing to their excellent mechanical properties and superior fatigue resistance. When machining these steels, severe tool wear frequently results in poor surface quality and low machining efficiency, which is intimately linked to the friction behavior at the tool–workpiece interface. To enhance the service life of tools, the adoption of efficient cooling methods is paramount. However, the understanding of friction behavior at the tool–workpiece interface under varying cooling conditions remains limited. In this work, both air atomization of cutting fluid (AACF) and ultrasonic atomization of cutting fluid (UACF) were employed, and their spray characteristic parameters, including droplet size distribution, droplet number density, and droplet velocity, were evaluated under different air pressures. Discontinuous sliding tests were conducted on the ultra-high-strength steel against cemented carbide and the effect of spray characteristic parameters on the adhesion friction coefficient was studied. The results reveal that ultrasonic atomization significantly improved the uniformity of droplet size distribution. An increase in air pressure resulted in an increase in both droplet number density and droplet velocity under both AACF and UACF conditions. Furthermore, the thickness of the liquid film was strongly dependent on the spray characteristic parameters. Additionally, UACF exhibited a reduction of 4.7% to 9.8% in adhesion friction coefficient compared to AACF. UACF provided the appropriate combination of spray characteristic parameters, causing an increased thickness of the liquid film, which subsequently exerted a positive impact on reducing the adhesion friction coefficient.
Evaluation on Preparation and Performance of a Low-Carbon Alkali-Activated Recycled Concrete under Different Cementitious Material Systems
Authors: Liu Cheng , Wang Xinyan , Li Yujiao , Li Qiuyi , Yue Gongbing
Published: October 2024

A green, low-carbon concrete is a top way to recycle waste in construction. This study uses industrial solid waste slag powder (S95) and fly ash (FA) as binders to completely replace cement. This study used recycled coarse aggregate (RCA) instead of natural coarse aggregate (NCA). This is to prepare alkali-activated recycled concrete (AARC) with different cementitious material systems. Alkali-activated concrete (AAC) mixtures are modified for strength and performance based on the mechanical qualities and durability of AARC. Also, the time-varying effects of the environment on AARC properties are explored. The results show that with the performance enhancement of RCA, the mechanical performance of AARC is significantly improved. As RCA’s quality improves, so does AARC’s compressive strength. At a cementitious material content of 550 kg/m3, AARC’s 28d compressive strengths using I-, II-, and III-class RCA were reduced by 2.2%, 12.7%, and 21.8%, respectively. I-class AARC has characteristics similar to natural aggregate concrete (NAC) in terms of shrinkage, resistance to chloride penetration, carbonization, and frost resistance. AARC is a new type of green building material that uses industrial solid waste to prepare alkali-activated cementitious materials. It can effectively reduce the amount of cement and alleviate energy consumption. This is conducive to the reuse of resources, environmental protection, and sustainable development.
Effect of Heating Rate on Hydride Reorientation Behavior of Zirconium Alloy Tubes under Non-Stress Loading
Authors: Hui Boning , Chen Mingju , Li Xinyi , Chen Biao , Li Yuli , Zhou Jun , Tang Rongtao , Li Jinshan
Published: October 2024

Zirconium alloys are widely used in nuclear water reactors as cladding materials. The cladding materials will absorb hydrogen from high temperature water during the operation of nuclear reactor. In cladding tubes, it has been common sense that circumferential hydrides form without stress, while radial hydrides can form when the hydrides are reoriented under stress loading. In this study, we found that a high heating rate can result in hydride reorientation behavior even without stress. At elevated heating rates, the zirconium alloy clad tube developed a non-uniform strain gradient along the direction of heat conduction. Hydrogen atoms migrate preferentially to areas of elevated stress and precipitate as hydrides that are perpendicular to the direction of tensile stress, resulting in the formation of radial hydrides that appear as “sun spots” macroscopically. Additionally, the high heating rate disrupts the {0001}α∥{111}δ, <11–20>α∥<110>δ orientation relationship between the hydride and the substrate, which potentially facilitates crack propagation.