bluuebunny/crossref_metadata_2025 huggingface dataset:
EFEK KOMBINASI ARTEMISININ DAN N-acetylcysteineTERHADAP KADAR Malondialdehyde(MDA) OTAK DAN PARU MENCIT GALUR Balb/c YANG DIINFEKSI Plasmodium berghei
Authors: Fitri Loeki Enggar , Iskandar Agustin , Permatasari Nur , Gunawan Joko Agus , Indrawan Khadafi
Published: January 2017

Cerebral and lung damage during malaria infection is believed to be caused by free radicals activitiesthat areÂ produced during immunology process. The free radicals react with lipid component of cellular membraneÂ which generates malondialdehyde (MDA) as its end-product. The aim of the research was to determineÂ whether combination of artemisinin and NAC was moreeffective in decreasing cerebral and lung MDA levelÂ compared to artemisinin mono-therapy . The researchwas post-test-control-only design using 5 groupsÂ consisted of group A (negative control group), group B mice which infected with P.berghei without therapyÂ (positive control group), group C mice which infected with P.berghei and received artemisinin mono-therapyÂ (0.04 mg/g BW for 7 days), group D mice which infected with P.berghei and received artemisinin inÂ combination with NAC (1 mg/g BW for 7 days) and group E mice which infected with P.berghei and receivedÂ artemisinin in combination with NAC (1 mg/g BW for 3 days and tapered into Â½ mg/g BW for 4 days). On theÂ 3rd, 5th,and 7thday, 3 mice from each group were scarified and assayed for MDA level. On the 3rdÂ day, aÂ decreasing trend of cerebral and lung MDA level wasobserved on all treatment groups. On the 5thday, aÂ decreasing trend of cerebral and lung MDA level wasobserved in group that received artemisinin and NACÂ whereas groupâ€™s that received artemisinin mono-therapy increased. Cerebral and lung MDA level of groupthat received artemisinin mono-therapy was significantly different with group that received combination ofÂ artemisinin and NAC in constant dose (p = 0.014) and with group that received combination artemisinin Â andÂ NAC in tapering dose (p = 0.004).
ANALISIS LOSS OF HETEROZYGOSITYGEN RAR Î²2 PADA PATOGENESIS KANKER PAYUDARA
Authors: Muliartha I Ketut Gede , Riawan Wibi , Hidayat Asnah , Armania Fitri
Published: January 2017

The aim of this research was to investigate the event of loss of heterozygosity of retinoid acid receptor (RARÂ Î²2) gene on the chromosom 3p24 in the pathogenesis ofbreast cancer. The breast cancer is the mostÂ malignant tumor that leads the second cause of death among the female. The recent concept of the theory ofÂ pathogenesis breast cancer is molecular genetic approaches such as apoptosis gene, oncogen, suppressorgene, and growth promoting factor gene. However, the diagnostic and therapy of breast cancer are still notÂ satisfied. The lost or decreased in the expressionof RAR Â Î²2gene on 3p24 chromosome via loss ofÂ heterozygosity (LOH) mechanism are the role of pathogenesis of breast cancer. Design of the research is byÂ experimental and observational. Human female breastcancer and blood samples were gained from publicÂ hospital RSUD dr. Saiful Anwar, Malang. DNA was extracted from lymphocyte and tissue using Macherey-Nagel system kit. Loss of heterozygosity (LOH) incident in 3p24 chromosome was detected by PCR andÂ Chromogenic in situ Hybridization (CISH) method.
EFEK 17Î²-ESTRADIOL TERHADAP DENSITAS RESEPTOR ADRENERGIK-Î±IDDAN KONTRAKTILITAS OTOT POLOS PEMBULUH DARAH TIKUS
Authors: Nurdiana Nurdiana
Published: January 2017

The incidence of hypertension in menopausal and post menopausal women related to plasma estrogen leveland sympathetic nervous activity. Estrogen may have a specific effect to modulate adrenergicÂ vasoconstriction by modulating adrenergic receptors. Blood vessels contractility is regulated mainly by theÂ sympathetic nervous system, in aorta mediated particularly by Â Î±1Dadrenergic receptor.We hypothesize thatÂ 17Î²estradiol decreases vascular smooth muscle contractility by decreasing Â Î±1Dadrenergic receptor density.Â To prove the above mechanism in the present study we performed several interrelated assays, i.e bioassayÂ using isolated organ and protein blotting. An isolated rat aortic ring without endothelium (2-3 month,150-200Â gram) was incubated in 17Î²-estradiol (10-5M, 10-4M,and 10-3M ) for two hours prior to stimulation usingÂ phenylephrine as Î±1Dadrenergic receptor agonist. The response of the rat aortic ring to the stimulation of Î±1Dadrenergic receptor agonist was measured as the subsequent change on aortic contractility using bioassay,Â and the change of Â Î±1Dadrenergic receptor density using Western and dotblot.
PEMBERIAN ETANOL JANGKA PANJANG MENURUNKAN MEMORI KERJA SPASIAL PADA TIKUS
Authors: Aswin Soedjono , Mustofa Mustofa
Published: January 2017

Long term ethanol administration may cause morphological changes in the hippocampus, followed by deficit inÂ  theÂ  hippocampalÂ  functionÂ  includingÂ  theÂ  impairmentÂ  ofÂ  memory.Â  TheÂ  aimÂ  ofÂ  thisÂ  studyÂ  isÂ  toÂ  investigateÂ  the changes of spatial working memory of rats after chronic ethanol administration at adolescent age. Twenty five adolescentÂ  (30Â  daysÂ  ofÂ  age)Â  maleÂ  ratsÂ  (RattusÂ  novergicus)Â  dividedÂ  randomlyÂ  intoÂ  5Â  groupsÂ  :Â  controlÂ  without treatment (C1), control with treatment (C2), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3) group. Each group consist of 5 rats. C2 group was given physiologic saline, T1, T2, and T3 group were given ethanol with dosesÂ  1,Â  2,Â  andÂ  3Â  g/kg/dayÂ  forÂ  30Â  daysÂ  byÂ  intraperitonealÂ  injection.Â  AfterÂ  chronicÂ  ethanolÂ  administrationÂ  the spatialÂ  workingÂ  memoryÂ  ofÂ  5Â  groupsÂ  wereÂ  testedÂ  byÂ  usingÂ  8Â  armÂ  radialÂ  mazeÂ  forÂ  12Â  days.Â  SpatialÂ  working memory measured by total number of entered arm maze and error A type. The results of this study showed thatÂ  chronicÂ  ethanolÂ  administrationÂ  atÂ  adolescentÂ  ageÂ  causedÂ  decreasingÂ  ofÂ  spatialÂ  workingÂ  memoryÂ  ofÂ  the rats. The total number of entered arms maze and error A type for treatment group (especially P3 ) is higher thanÂ  controlÂ  groupsÂ  (p<0,05).Â  ItÂ  isÂ  concludedÂ  thatÂ  inÂ  rats,Â  chronicÂ  ethanolÂ  administrationÂ  atÂ  adolescentÂ  age caused decreasing spatial working memory.Â  Â Key words : Ethanol, Spatial working memory, Adolescent
JUMLAH SEL TROFOBLAS YANG MENGALAMI APOPTOSIS PADA PRE EKLAMPSIA/EKLAMPSIA LEBIH TINGGI DIBANDINGKAN KEHAMILAN NORMAL
Authors: Keman Kusnarman , Prasetyorini Nugrahanti , Langgar Madeline J.
Published: January 2017

Hypoxia exposed trophoblast cells will cause apoptotic process, that correlate with up-regulation of p53 andÂ Bax protein expression; and down-regulation of anti-apoptotic Bcl-2 protein. Apoptotic process and up-regulation of p52, and down-regulation of anti-apoptotic Bcl-2; also find both on trophoblastic samplefromÂ preeclampsia/ eclampsia complicated pregnancy, and Â normal pregnancy. The aim of this study is, to proveÂ that the amount of apoptotic trophoblast cells on trophoblast tissue sample from preeclampsia/ ecclampsiaÂ complicated pregnancy, were higher then those of normal pregnancy. This research was laboratory crossÂ sectional study, that observed immunohystochemically, p53 and BCl-2 protein expression; also the DNA-fragmented technically (TUNEL) to detect apoptotic Â cells on trophoblastic samples from preeclampsia/Â eclampsia complicated pregnancy, compare by the normal pregnancy. On this study, we found that theÂ amount of trophoblast cells that expressed of p53 protein (22.70 Â± 2.90), were higher then those of normalÂ pregnancy. (8.2 Â± 4.99). The different was statistically significant. (t test; p Â â‰¤0.000). We also found that anÂ apoptotic process on trophoblast cells of preeclampsia/ eclampsia placenta (15.3 Â± 3,56) were higher Â thenÂ those of normal pregnancy (4.7 Â± 1.89). The different was statistically significant. (t test; p â‰¤0.000).
EFEK EKSTRAK BUAH TOMAT (Licopersicum pyriforme) TERHADAP EKSP KOLAGEN TIPE 1, MMP-1 DAN MMP-3 PADA PENUAAN KULIT
Authors: Wahyono Poncojari
Published: January 2017

ABSTRACT ManyÂ  theoryÂ  andÂ  researchÂ  wasÂ  tryingÂ  toÂ  explainÂ  theÂ  agingÂ  process.Â  FreeÂ  radicalÂ  theoryÂ  explainedÂ  thatÂ  allnormal metabolism process in the body can produce free radical.These free radical can destroy the skin cell,andÂ  thanÂ  theÂ  skinÂ  willÂ  startÂ  theÂ  agingÂ  process.Â  TheÂ  tomatoÂ  extractÂ  hasÂ  antioxidantÂ  suchÂ  asÂ  lycopene,Â  Î²-caroteneÂ  andÂ  vitaminÂ  C.Â  TheseÂ  antioxidantÂ  willÂ  preventÂ  theÂ  activityÂ  ofÂ  freeÂ  radical,Â  soÂ  thatÂ  skinÂ  cellÂ  willÂ  notdamage.Â  TheÂ  aimsÂ  ofÂ  thisÂ  researchÂ  wereÂ  toÂ  studyÂ  effectÂ  ofÂ  tomatoÂ  extractÂ  juiceÂ  inÂ  preventingÂ  skinÂ  agingÂ  onwistar rats. Total of 25 rats were divided into 5 groups, consisted of 5 rats each. Group one was control groupD-0) rats were four months old, and the group two control of group rats were taken care of 4 months old until7Â  monthsÂ  oldÂ  (D-1),Â  theÂ  otherÂ  groupÂ  whichÂ  wereÂ  underÂ  treatmentÂ  classifiedÂ  intoÂ  3Â  groups.Â  GroupÂ  threeÂ  wereed 7 g/kg/day/ body weight of tomato fruit extract, then taken care of 4 months old until 7 months old (D-2),group four were fed 11 g/kg/day/ body weight of tomato extract fruit, then taken care of 4 months old until 7months old (D-3) and group five were fed 15 g/kg/day/ body weigh of tomato fruit extract, then taken care of 4monthsÂ  oldÂ  untilÂ  7Â  monthsÂ  oldÂ  (D-4).
ADIPONEKTIN MEREGULASI TURUN EKSPRESI iNOS PADA KULTUR HUVECâ€™s YANG DIPAPAR LIPO POLYSACCHARIDE (LPS)
Authors: Hernani Yully Endang
Published: January 2017

ABSTRACT AdiponectinÂ  isÂ  aÂ  adipocyteÂ  derivateÂ  proteinÂ  involvesÂ  inÂ  sensitivityÂ  ofÂ  insulin,Â  anti-inflamation,Â  andÂ  anti-atherogenicÂ  foundÂ  largelyÂ  inÂ  plasma.Â  TheÂ  decreaseÂ  ofÂ  adiponectinÂ  inÂ  theÂ  plasmaÂ  isÂ  relatedÂ  toÂ  obesity, cardiovascular diseases, type II diabetes, hypertension and vascular injuries in nice. In vivo research on strain balb/c mice showed that excessive Adiponectin expression inhibits atherosclerosis with Apo E deficiency. The aimÂ  ofÂ  thisÂ  researchÂ  isÂ  toÂ  findÂ  protectiveÂ  mechanismsÂ  ofÂ  adiponectinÂ  onÂ  vascularÂ  utilizingÂ  in-vitroÂ  Infected HUVECâ€™sÂ  culture.Â  ThereÂ  wereÂ  5Â  groupsÂ  :(A)Â  controlÂ  negativeÂ  groupÂ  orÂ  normalÂ  HUVECâ€™s,
IDENTIFIKASI MUTASI GEN CYSTATIONINE-Î² SYNTHASE (CBS) DAN GEN METHYLENETETRAHYDROFOLATE REDUCTASE (MTHFR) PADA PENDERITA PREMATUR INFARK MIOKARD AKUT DENGAN HIPERHOMOSISTEINEMI
Authors: Muliartha I Ketut Gede , Sargowo Djanggan , Iskandar Abdillah
Published: January 2017

ABSTRACT Hyperhomocysteinemia appears to be an independent risk factor for coronary heart disease. Elevated levels ofÂ  plasmaÂ  totalÂ  homocysteineÂ  (tHcy)Â  wasÂ  causedÂ  byÂ  geneticÂ  orÂ  nutrient-relatedÂ  disturbancesÂ  inÂ  the transsulfurationÂ  orÂ  remethylationÂ  pathwaysÂ  forÂ  homocysteineÂ  metabolism.Â  ThisÂ  studyÂ  observedÂ  premature myocardÂ  infarctÂ  acuteÂ  patientsÂ  withÂ  hyperhomocysteinemia.TheÂ  aimÂ  ofÂ  theÂ  researchÂ  wasÂ  toÂ  determine Cystarhionine-Î²Â  SynthaseÂ  andÂ  MethylenetetrahydrofolateÂ  ReductaseÂ  genesÂ  mutationsÂ  inÂ  prematureÂ  acute myocardÂ  infarctÂ  patientsÂ  withÂ  hyperhomocysteinemia.ThisÂ  studyÂ  wasÂ  aÂ  crossÂ  sectionalÂ  studyÂ  inÂ  premature acute myocard infarct patients. Twenty three patients were examined for plasma total homocysteine, vitamin B6, vitamin B12 folic acid and lipid profile. Total DNA isolated from patients with hyperhomocystememia and normalÂ  folicÂ  acid,Â  vitaminÂ  B6,Â  vitaminÂ  B12Â  levelsÂ  andÂ  lipidÂ  profile.Â  FiveÂ  patientsÂ  wereÂ  assayedÂ  for Cystathionine-Î²Â  SynthaseÂ  andÂ  genÂ  MethylenetetrahydrofolateÂ  ReductaseÂ  geneseÂ  mutationsÂ  byÂ  Polymerase ChainÂ  ReactionsÂ  (PCR).Â  FiveÂ  fromÂ  23Â  patientsÂ  (Â  21,7%Â  )Â  plasmaÂ  total
STUDI KUALITATIF PENGAMBILAN KEPUTUSAN DALAM KELUARGA TERKAIT DENGAN KOMPLIKASI PERINATAL DI KABUPATEN CIANJUR JAWA BARAT
Authors: Melani Asti
Published: January 2017

ABSTRACT The high number of maternal mortality rate caused by 3 complex problems. One of those problems was delay identificationÂ  byÂ  theÂ  familyÂ  onÂ  theÂ  riskÂ  factorsÂ  ofÂ  theÂ  maternalÂ  andÂ  makeÂ  decisionÂ  inÂ  seekingÂ  helpÂ  relatedÂ  to their problem. The goal of this study was to identify how the family involvement role, especially their husband, to the maternal health and how the decision making pattern in the family, related to the perinatal complication. ThisÂ  studyÂ  isÂ  qualitativeÂ  researchÂ  usingÂ  phenomenologyÂ  approach.Â  TheÂ  numberÂ  ofÂ  participantÂ  inÂ  thisÂ  study were six couples husband and wife in Bojongherang, Cianjur, West Java. The findings identified that most of the decision maker in the family were the husbands, except those wife who are the carrier woman. In general, the process of decision making start with discussion from both husband and wife before the decision making is made. Some other is decided by their husband instead. The most influenced factors to the decision making process were socio-economic, accessibility to the health insurance and the family knowledge on the perinatal complications.Â  TheÂ  otherÂ  lessÂ  influencedÂ  factorsÂ  wereÂ  gender,Â  educationalÂ  backgroundÂ  andÂ  cultural background. Â Key words : Decision making in the family,perinatal complication, maternal health.
HUBUNGAN TINGKAT PENGETAHUAN DENGAN PARTISIPASI PADA PEMERIKSAAN PAP SMEAR PADA WANITA PEKERJA SEKS KOMERSIAL
Authors: Kurniawan Bayu , Asmika Asmika , Sarwono Imam
Published: January 2017

ABSTRACT Carcinoma cervix is the most frequent malignancy and the highest cancer mortality rate in women worldwideas well as in Indonesian. From 4.290 of cervical carciona in Indonesia , 1.326 cases were found in SurabayaOver 70% patients were in the advanced stadium. Pap Smear as a screening test is a prevention strategy focarcinomaÂ  cervix.Â  SexÂ  workerÂ Â Â  hasÂ  aÂ  highÂ  riskÂ  forÂ  carcinomaÂ  cervix.Â  ThisÂ  researchÂ  wasÂ  carriedÂ  outÂ  inÂ  GangDollyÂ  ProstitutionÂ  AreaÂ  SurabayaÂ  aimedÂ  toÂ  describeÂ  theÂ  characteristicsÂ  ofÂ  sexÂ  workerÂ  andÂ  theÂ  correlationbetweenÂ Â Â  knowledgeÂ  levelÂ  andÂ  participationÂ  inÂ  PapÂ  SmearÂ  examinationÂ  .Â  AÂ  crossÂ  sectionalÂ  approachÂ  wasconducted withÂ  67 respondents. The variables: characteristics, knowledgeÂ  level and pap-smearÂ  participationwasÂ  measuredÂ  byÂ  openÂ  andÂ  close-endedÂ  questionnaireÂ  guidedÂ  byÂ  theÂ  researcher.Â  TheÂ  dataÂ  wereÂ  presentedasÂ  crossÂ  tabulationÂ  andÂ  furtherÂ  testingÂ  theÂ  correlationÂ  basedÂ  onÂ Â Â  LambdaÂ  CorrelationÂ  testÂ  (Î±Â  =Â  0,05).Â  TheresultsÂ  showedÂ Â Â  anÂ  equalÂ  proportionÂ  ofÂ  goodÂ  andÂ  sufficientÂ  knowledgeÂ  andÂ  onlyÂ  limitedÂ  respondentÂ  (2,99%whoÂ  haveÂ  veryÂ  poorÂ  knowledge.Â  89,55%Â  respondentsÂ  haveÂ  hadÂ  doneÂ  theirÂ  PapÂ  SmearÂ  examination,Â  fromthoseÂ  mostlyÂ  wereÂ  respondentsÂ  withÂ  sufficientÂ  knowledgeÂ  (41,79%).Â Â Â  ThereÂ  wasÂ  aÂ  weakÂ  andÂ  notÂ  significancorrelationÂ  betweenÂ  respondentâ€™sÂ  knowledgeÂ  levelÂ  aboutÂ  carcinomaÂ  cervixÂ  andÂ  theirÂ  participationÂ  inÂ  PapSmear examination (Lambda Correlation test, Lb = 0,286, approx. sig. = 0,151). Â Keywords : Knowledge , Carcinoma Cervix, Pap Smear, Participation
TERAPI BIOLOGIK PADA PASIEN PSORIASIS
Authors: Thaha Athuf
Published: January 2017

ABSTRACT TreatmentÂ  limitationsÂ  ofÂ  currentÂ  immunosuppressiveÂ  therapies,Â  andÂ  growingÂ  understandingÂ  ofÂ  psoriasispathogenesis on a molecular level have stimulated much interest in the field of immunomodulatory therapy fomanagement of psoriasis. Genetic engineering has allowed the development of proteins that are targeted tospecific cell surface receptors. A well over 40 targeted biologic agents are in preclinical and clinical testing foT-cellÂ  mediatedÂ  inflammatoryÂ Â Â  diseases,Â  includingÂ  psoriasis.Â  ItÂ  isÂ  importantÂ  that,Â  asÂ  aÂ  doctorÂ  becomeÂ  weleducated in biologics, since several of these agents are available for the management of psoriasis.Â  Â Keywords:Â  immunosuppressiveÂ  therapy,Â  immunomodulatoryÂ  therapy,Â  geneticÂ  engineering,Â  surfaceÂ  receptors,Â  targetedbiologic agents, biologics.
ENCEPHALOPATY AKUT PADA ANAK DENGAN KELAINAN METABOLISME BAWAAN (PENDEKATAN PRAKTIS)
Authors: Puryatni Anik , De Koning Tom J.
Published: January 2017

ABSTRACT AcuteÂ  encephalopathyÂ  isÂ  aÂ  notÂ  aÂ  common,Â  butÂ  aÂ  potentialÂ  emergencyÂ  situationÂ  inÂ  newbornsÂ  andÂ  youngchildren.Â  IfÂ  theÂ  encephalopathyÂ  isÂ  notÂ  causedÂ  byÂ  asphyxiaÂ  orÂ  infectionsÂ  ofÂ  theÂ  brain,Â  rareÂ  inbornÂ  errorsÂ  ofmetabolism need to be considered. An acute encephalopathy due to an inborn error can occur in newborns,youngÂ  infants,Â  orÂ  evenÂ  inÂ  childhoodÂ  andÂ  isÂ  notÂ  alwaysÂ  easilyÂ  recognizedÂ  becauseÂ  moreÂ  subtleÂ  presentationscanÂ  occur.Â  InÂ  thisÂ  articleÂ  weÂ  willÂ  giveÂ  aÂ  practicalÂ  guidelineÂ  toÂ  theÂ  diagnosticÂ  approachÂ  ofÂ  metabolicÂ  diseasespresenting with acute neurological symptoms with an emphasis on treatable disorders and the application indevelopingÂ  countriesÂ  withÂ  limitedÂ  diagnosticÂ  resources.Â  TheÂ  firstÂ  stepÂ  inÂ  theÂ  evaluationÂ  ofÂ  patientÂ  withÂ  apossibleÂ  metabolicÂ  diseaseÂ  isÂ  toÂ  categoriseÂ  theÂ  clinicalÂ  appearanceÂ  ofÂ  theÂ  patientÂ  inÂ  oneÂ  ofÂ  theÂ  followingclinical categories: Hypoglycemia phenotype; Intoxication phenotype; Neurotransmitter defect phenotype; andCellular energyÂ  metabolism defectÂ  phenotype.Â  Second, combine this clinical classification with your physicaexaminationÂ  andÂ  theÂ  resultÂ  ofÂ  routineÂ  laboratoryÂ  investigationsÂ  andÂ  thisÂ  canÂ  leadÂ  toÂ  suspectedÂ  diagnosisÂ  ofmetabolicÂ  disorders.PromptÂ  recognitionÂ  andÂ  treatmentÂ  areÂ  important,Â  becauseÂ  anÂ  acuteÂ  metabolicencephalopathy can irreversible and interruption of normal neural activity in the developing brain can have along-lastingÂ  effectÂ  onÂ  psychomotorÂ  development.Â  DelayÂ  inÂ  diagnosisÂ  andÂ  treatmentÂ  mayÂ  thusÂ  resultÂ  inÂ  acutemetabolic decompensation, progressive neurologic injury, or even death. Â Key words: acute encephalopathy, inborn error metabolisme
Pengembangan Model Mencit Alergi dengan Paparan Kronik Ovalbumin
Authors: Barlianto Wisnu , Kusuma Mohamad Slamet Chandra , Karyono Setyawati , Mintaroem Karyono
Published: January 2017

ABSTRACTHistology expression in asthma show inflammatory processes and airway structural changes. Treatmentdevelopment in asthma needs appropriate animal models but most animal models use short term allergenexposure. The aim of this research was to create develop allergic mouse model with allergic inflammationand structural changes histology expression. Eight female BALB/c mice, 6-12 weeks old, 80-100 gramswere divided into two groups. The first group were exposed to a normal saline solution where the secondgroup received ovalbumin. Mice were sensitized by intraperitoneal injection of ovalbumin on day 0 and 14and challenged by nebulization of 1% ovalbumin three times weekly for 6 weeks. Twenty four hours afterfinal exposure, the mice were sacrificed by lethal cardiac puncture. The level of total IgE in serum weremeasured by ELISA and IL-4 receptor expression were examinated by immunohistochemistry.Morphometric analysis was performed to count inflammatory cells, eosinophil, goblet cells, epithel andsmooth muscle thickness. Differences between the control and exposed mice were analyzed by Mann-Whitney test. There were a significant differences in total IgE serum and expression IL-4 receptor betweenthe two groups (P < 0,05). The morphometric analyzing also demonstrated significant differences betweenthese two groups (P < 0,05). Chronic ovalbumin exposure through inhalation produced allergicinflammation and airway structural changes.Keywords: allergic inflammation, mouse models, chronic exposure, ovalbumin
Aromaterapi Bunga Lavender Memperbaiki Kualitas Tidur pada Lansia
Authors: Kurnia Anggraini Dwi , Wardhani Viera , Rusca Kuswantoro Tri
Published: January 2017

Sleep is a basic human need, characterized by minimal physical activity, change in physiological process ofthe body and decrease respon from external stimulus. Most of the elderly have high risk to suffer sleepproblem because of some factors. Aromatherapy is a therapy using essential oil which produces comfortableeffect, more steady emotion and feeling, calmer thought and feeling. This research was aimed to identify theeffectivity of Lavender (Lavendula L) Aromatherapy in improving quality of sleep of the elderly in Panti WerdhaMalang. A quasi experiment study was implemented with randomized control group pretest posttest using 18subjects divided into two groups.and treatment. The sleep quality was measured by interview developedbased on PSQI (Pittsburgh Sleep Quality Index). The data were then analyzed using Wilcoxon test and Mann-Whitney test. A significant improvement of sleep quality score was identified in treatment group (3,89, p=0,07) but not in the control group (0,78;p=0,317). These improvement is significantly different between the twogroups (p=.0,001). It can be concluded that Lavender aromatherapy is significantly improve the quality ofsleep in elderly people
Susu Kedelai Menurunkan Resistensi Insulin pada Model Diabetes Melitus Tipe 2
Authors: Handayani Wiwik , Rudijanto Ahmad , Indra Mohammad Rasjad
Published: January 2017

Type 2 Diabetes mellitus (T2DM) is characterized by hyperinsulinemia and hyperglicemia. Soy bean Milk ishigh nutrient drink, especially its protein contain. Several researches demonstrated that soy bean proteindecrease plasma insulin and blood glucose level. The aim of this study is to investigate the effect of soy beanmilk in reducing insulin resistance of rat T2DM Model. This experimental study used pre and post test controlgroup design. Male Rattus norvegicus wistar strain 8 week old were used as animal model. T2DM rats wereconstructed by giving high fat diet and continue with injection of low dose of streptozotocin. Rats were dividedinto 7 groups (1) Negative control group (2) Positive control group (T2DM rat without soy bean milk) (3) T2DMrat that treated with genistein 15 g/kgBW (4) T2DM rat that treated with soy bean milk 10 ml/kgBW/day (5)DMT2 rat that treated with soy bean milk 30 ml/kgBW/day (6) T2DM rat that treated with soy bean milk 60ml/kgBW/day and (7) T2DM rat that treated with soy bean milk 90 ml/kgBW/day.
Identifikasi Forensik Berdasarkan Pemeriksaan Primer dan Sekunder Sebagai Penentu Identitas Korban pada Dua Kasus Bencana Massal
Authors: Prawestiningtyas Eriko , Algozi Agus Mochammad
Published: January 2017

ABSTRACTThe main purposes of identification process in mass disaster is to find the corpses identity in order to give itback to their family by doing some identification methods such as primer identification which must do at thefirst time because they have highly and accurate result as the identifiers. There are many differencesbetween the burned corpses on the the tragedy of the burning of Garuda Airlines and the drowned corpses onthe tragedy of the sinking of Senopati Nusantara ship , many difference characteristic that influence theprocess of forensic identification. In the burned corpses, teeths are still intact and relatives still could beexamined than another primery identifiers. It makes difference priority between one to another cases. So,from the two different cases, we can choose the different primer identifiers as the priority depend on thecondition of the dead bodies related to the accident in order to make the right identity for giving back to therelatives.
Penggunaan Suksinilkolin Setelah Tidak Meningkatkan Kadar Kalium Plasma Pasien dengan Cedera Kepala Berat
Authors: Purwati Wahyuni Dian , Wardhani Viera , Mahameru Gunung , Istiadjid Mohamad
Published: January 2017

This study aimed to identify the changes of plasma potassium level after intubation with rapid sequenceintubation method in severe head injury patients using succinylcholine as muscle relaxant. Subjects aresevere head injury patients with glasgow coma scale (GCS) â‰¤ 8. Plasma potassium level was measured twice,before intubation and (minutes 5, 10, 15, 20) after intubation. Another variable that also measured were bloodgas analyze, time intubated and injury in another organ. There were 34 patients who came to EmergencyDepartment (ED) of Saiful Anwar General Hospital since January until April 2009. Mean level of plasmapotassium changes after intubation minute 5 (-0.01529); minute 10 (-0.01971); minute 15 (0.04147); minute20 (0.00059). Statistical analysis using paired t test revealed no significance of this difference (p>0.05). Therewere no changes of plasma potassium level in severe head injury patients after intubation withsuccinylcholine
Outer Membrane Protein 49,4 Kda dari Porphyromonas gingivalis Merupakan Protein Hemaglutinin dan Adhesin Terhadap Netrofil
Authors: Mubarokah Siti Nurul , Sumarno Sumarno , Muliartha I Ketut Gede
Published: January 2017

ABSTRACTPeriodontitis is caused mostly by P. gingivalis and it is related to acute coronary syndrome. P. gingivalis readilyinvades into blood circulation and potentially induces. Collagenolytic activity of neutrophil which result incollagen vascular degradation lead to atherosclerotic plague rupture (APR). APR is responsible for occurringfatal cardiovascular events such as acute myocardial infarction (AMI). These information brought out notionconcerning the adhesion interaction of P. gingivalis with neutrophil. The aim of this study was to assessadhesion molecule of P. gingivalis outer membrane protein (OMP) by partial characterization took inhemagglutination assay using mice erythrocytes, adhesion inhibition assay by gradual concentration ofadhesion blocked in neutrophil, immunologic assay using Western-blotting and immunocytochemistry. Theresults showed that 49,4 kDa P.gingivalis OMP can agglutinate mice erythrocytes and adherence to netrophilIncrease concentration of OMP P. gingivalis 49,4 kDa reduce adhesion process to netrophil This protein wasrecognized by the polyclonal antibody of 49,4 kDa adhesion molecule. It can be concluded that P gingivalisouter membrane protein is a hemagglutinin and an adhesion molecule to netrophilKeywords : 49,4 kDa P. gingivalis OMP, neutrophil, hemagglutination, adhesin
Induksi of Porphyromonas gingivalis Meningkatkan Proporsi Endotelin-1 pada Aorta Mencit yang Diinduksi Diet Aterogenik
Authors: Elyani Helmin Elyani , Hernowati Tinny Endang , Sumarno Sumarno
Published: January 2017

One of the risk factor of atherosclerosis is associated with activation of the inflammatory process in infection,as evidence by systemic elevations of molecules such as MCP-1,PAI-1 and ET-1. Recent evidence suggeststhat infectious agents, including those that cause periodontal disease, may also important role inatherosclerosis. The aim of this study was to determine if recurrent intravenous injections with P. gingivalis for14 weeks promotes endothelial smooth muscle ET-1 proportion in aortic mice. Twenty four weeks old mice(n=6 per group for each time point) were divided in two groups, there were normal diet and atherogenic diet,each of them were inoculated intravenously with live P. gingivalis (10 cell/50Âµl PBS) injections or vehicle (50Âµl PBS) once per week for 14 weeks. The Immunohistochemistry for aortic ET-1 proportion were performed.The result of RAK with 3 interaction and Tukey test using SPSS 14 for windows showed that at 14 weeks, theET-1 proportion in aortic endothelial cell was higher than in aortic smooth muscle cell.
Perbedaan Pola Peningkatan Suhu Bahan Biologis dan Non-Biologis yang Dipapar Sinar Far-Infrared
Authors: Soleh Miftahu , Sujuti Hidayat , Widjajanto Edi
Published: January 2017

Low Â power Â of Â Far-Infrared Â Rays Â (FIR) Â emitted Â by Â artificial Â radiators Â have Â been Â used Â for Â many Â diseasesÂ associated with blood vessels disorder. Â FIR also used to Â regulate Â temperature Â in Â incubator Â for Â infant. Â ThisÂ study aims at exploring correlation pattern between FIR and temperature, Â producing a biological simulatorÂ model from Â a Â non-biological Â material for Â further Â research Â can Â be Â initiated. Â Differentiation Â factors Â betweenÂ biological Â and Â non-biological Â material Â that Â exposed Â to Â FIR Â also Â could Â be Â determined. Â A Â radiant Â far-infrared Â typeÂ T -FSR Â was placed 20 cm Â above the Â objects (umbilicus Â and gel). Then both surface Â and inside Â temperature Â ofÂ the Â objects Â measured Â continuously Â using Â Thermistor Â series Â D-S06 Â and Â series Â D-RB2. Â Room Â humidityÂ maintained at constant levels Â (60%). Â This study found Â a significant correlation between FIR and temperatureÂ increasing Â both on the Â umbilicus and on the Â gel. Influence Â of FIR intensity Â on temperature Â can be formulatedÂ into Â the Â formula: Â T_umb_U1 Â = Â 27,6 Â + Â 0,00554 Â FIR Â and Â T_umb_U2 Â = Â 27,8 Â + Â 0,00560 Â FIR, Â while Â on Â the Â gel Â wereÂ T_gel_U1 = 24,9 + 0,00430 FIR and T_gel_U2 = 25,0 + 0,00428 FIR.
Peningkatan Ekspresi Interleukin (IL)-4 Berhubungan dengan Penurunan Bax dan Apoptosis Limfosit pada Bronkiolus dan Paru Mencit Asma
Authors: Yuliarto Saptadi , Kusuma HMS Chandra , Widjajanto Edi
Published: January 2017

Decrease of lymphocyte Â apoptosis is Â one factor Â that Â leads Â to Â chronic airway inflammation Â in Â allergic asthma.Â Previous Â studies Â revealed Â the Â role Â of Â interleukin Â (IL)-4 Â in Â preventing Â lymphocyte Â apoptosis; Â however Â there Â areÂ few Â studies Â about Â the Â role Â of Â lymphocyte-Bax Â and Â its Â relationship Â with Â IL-4 Â in Â asthma. Â The Â aim Â of Â the Â study Â is Â toÂ demonstrate IL-4 role in preventing lymphocyte apoptosis via lymphocyte-Bax inactivation in bronchioli andÂ lungs Â of Â asthmatic Â mice. Â It Â is Â a Â randomized Â control Â group Â experimental Â study . Â The Â subject Â was Â Balb/c Â mice Â thatÂ categorized Â into Â 2 Â groups: Â asthma Â and Â non-asthma. Â Asthma Â group Â was Â sensitized Â by Â ovalbuminÂ intraperitoneally Â in Â day Â 0 Â and Â 14, Â followed Â by Â inhalation Â every Â 2-3 Â days Â for Â 6 Â weeks. Â In Â week Â 8, Â all Â of Â mice wereÂ terminated. Â The Â IL-4 Â and Â lymphocyte-Bax Â expression Â was Â examined Â through Â immunohistochemistry Â method,Â whereas Â lymphocyte Â apoptosis Â by Â TUNEL Â method. Â Independent Â sample Â t-test, Â Mann Â Whitney Â U Â test,Â regression Â analysis, Â and Â path Â analysis Â was Â used Â in Â statistical Â analysis Â with Â confident Â interval Â 95%. Â TheÂ bronchioli and lungs specimens were obtained from 18 mice (9 in each group). Lymphocyte apoptosis wasÂ similar between 2 groups (p=0.1 16), additionally lymphocyte-Bax Â decreased in Â asthmatic group (p=0.003).Â This Â indicated Â low Â activity Â of Â lymphocyte Â apoptosis Â in Â asthmatic Â group.
Penundaan Makanan Padat Mengurangi Risiko Timbulnya Atopi pada Anak
Authors: Barlianto Wisnu , Kusuma HMS Chandra , HM Ni Luh Putu
Published: January 2017

Nutritions Â plays Â an Â important Â role Â in Â the Â incidence Â of Â atopic Â both Â in Â children Â and Â adults. Â Several Â recentÂ guidelines Â recommend Â a Â delay Â of Â solid Â foods Â to Â prevent Â atopic Â disease. Â However, Â until Â now, Â there Â is Â still Â muchÂ controversy Â about Â the Â influence Â a Â delay Â of Â solid Â foods Â in Â children Â to Â develop Â atopic Â  disease. Â This Â study Â aimedÂ to Â determine Â the Â impact Â of Â the Â start Â time Â of Â solid Â foods Â to Â atopic Â children. Â A Â case-control Â study Â was conductedÂ in Â outpatient Â institutions Â Pediatrics RSSA Â Malang from Â May 2008 Â until Â May 2009. Â Respondents were dividedÂ into two groups: atopic (cases) and non atopic (control) based on history of atopic disease. The age of firstÂ given Â solid Â food Â and Â familial Â atopic Â were retrieved Â based Â on Â history . Â Statistical analysis Â using Â Chi-square testÂ at 95% Â  Â level Â of confidence, Â was conducted Â to Â determine the Â impact Â of the Â start Â time Â of solid Â food Â to Â theÂ incidence Â of Â atopic. Â There were 236 Â patients Â divided Â equally Â into Â atopic Â and Â control Â group. Â Giving solid Â foodsÂ at 4-6 months of age will increase Â the Â risk Â of atopic compared Â with age> 6 months (OR Â 2.8 (1.29 Â to Â 6.07), p =Â 0.007). Â The Â results Â of Â this Â study Â support Â the Â recommendations Â given Â by Â WHO, that Â the Â postponement Â of Â solidÂ food Â until Â age Â 6 Â months Â would Â reduce Â the Â incidence Â of Â atopic Â risk Â in Â children.Keywords Â : Â postponement Â , Â solid Â food, Â atopic Â diseases, Â sensitization
Peningkatan Jumlah Apoptosis Airway Smooth MuscleBerhubungan dengan Peningkatan + Jumlah Sel T CD8 pada Model Mencit Asma
Authors: Setiawati Dessy , Kusuma HMS Chandra , Kusworini Kusworini
Published: January 2017

Airway Â remodeling Â is Â an Â important Â thing Â in Â asthma.The Â increase Â of Â airway Â smooth Â muscle Â mass Â is Â aÂ prominent Â feature Â of Â asthmatic Â airway Â remodeling. Â The importance Â role Â of Â airway Â smooth Â muscle cell Â (ASM)Â +Â apoptosis in airway remodeling is unknown.T cell also had important role in asthma, whereas CD4 T cellÂ +role Â in Â airway Â remodeling Â has Â already Â known, Â but Â how Â CD8 Â  T cell Â role Â in Â airway Â remodeling Â is Â still Â unclear. Â AÂ randomized Â control Â group experimental study Â used Balb/c mice, that Â categorized into Â 2 groups: asthma andÂ control. Â Asthma group was sensitized by ovalbumin intraperitoneally Â in Â day 0 and 14, followed Â by inhalationÂ +every Â 2-3 Â days Â for Â 6 Â weeks. Â In Â week Â 8, Â all Â of Â mice Â were Â terminated. Â  The Â expression Â of Â CD8 Â  T Â cell Â lymphocyteÂ was Â examined Â through Â immunohistochemistry Â method, Â whereas Â ASM Â apoptosis Â by Â TUNEL Â method.Â Independent sample t-test and spearman test was used in statistical analysis with confident interval 95%.Â The Â bronchioli Â and Â lungs Â specimens Â were Â obtained Â from Â 18 Â mice Â (9 Â in Â each Â group). Â Case Â goup Â had Â significantÂ +increase Â in Â the Â amount of ASM apoptosis and expression of CD8 Â  T cell lymphocyte Â (p=0.000; Â p=0.001).Â +There was also positif correlation between Â ASM apoptosis and expression of CD8 Â  T cell (r=0.37, Â p=0.065).Â +We Â conclude Â that Â increasing Â ASM Â apoptosis Â has Â a Â relationship Â with Â increasing Â CD8 Â  T Â cell+Keywords: Â asthma, Â mice, Â ovalbumin, Â ASM Â apoptosis Â and Â CD8 Â  T Â cell
Ekspresi dan Aktivasi Reseptor Glukokortikoid serta Ekspresi Heat Shock Protein- 90 pada Sel Limfosit Penderita Sindrom Nefrotik Idiopatik
Authors: Khairiyadi Khairiyadi , Subandiyah Krisni , Fitri Loeki Enggar
Published: January 2017

Glucocorticoid receptors (GR) have an important role in the resistance of steroid therapy . Previous studiesÂ showed Â significant Â difference Â activation Â and Â expression Â of Â GR Â between Â Steroid Â Resistant Â NephroticÂ Syndrome (SRNS) and Steroid Sensitive Nephrotic Syndrome (SSNS), however the other studies showedÂ contrastive result. Previous studies also revealed Hsp-90 had an important Â role in Â GR activation. The aim ofÂ this study was to determine the differences expression and activation of GR and also Hsp-90 expression inÂ lymphocytes Â cytoplasm Â and Â nucleus Â between Â SRNS Â and Â SSNS Â patients. Â A Â Cross-sectional Â study Â wasÂ conducted Â to Â 40 Â children Â (20 Â SRNS Â and Â 20 Â SSNS Â patients). Â Double Â stainning Â immunocytochemistry Â ofÂ lymphocytes Â was Â performed. Â The Â GR Â expression Â and Â activation Â were Â determined Â by Â counting Â amount Â ofÂ lymphocytes Â that Â express GR and Hsp-90 among 200 under light Â microscope with 1000x magnification. TheÂ differences Â of Â activation Â and Â activation Â of Â GR Â and Â Hsp-90 Â in Â cytoplasm Â and Â nucleus Â were Â analyzed Â withÂ independent t-test and the correlation was analyzed with Pearson correlation.
Pengobatan Suportif Vitamin D Mempercepat Konversi Sputum dan Perbaikan Gambaran Radiologis Penderita T uberkulosis
Authors: Siswanto Siswanto , Sumarno Sumarno , Jane Yani , Widayanti Oeryana Agustin , Muktiati Nunuk Sri
Published: January 2017

Pulmonary T uberculosis remains Â as a major health problem in Â South East Asia as well as Indonesia. Â TheÂ failure Â of Â tuberculosis Â treatment Â is Â mostly Â due Â to Â drop-out Â and Â multiple Â drugs Â resistant. Â Previous Â study Â showedÂ that Â Vitamin Â D Â increase Â the Â intracellular Â cathelicidin Â which Â has Â potency Â to Â kill Â the Â Mycobacterium tuberculosis.Â This research was aimed to identify the effect of Vitamin D as supportive treatment Â on the improvement ofÂ sputum Â conversion, Â radiographic Â finding Â and Â TNF-Î± Â serum Â concentration. Â A Â Double-blind randomized Â controlÂ trial Â was Â used Â in Â two Â random Â allocated Â patient Â group Â with Â standard Â tuberculosis Â treatment. Â  The Â first Â group Â wasÂ treated Â with Vitamin Â D (800 Â iu/day) Â while the Â other was not. Subjects were evaluated using three Â parametersÂ i.e. Â sputum Â conversion, Â radiographic Â finding Â and Â TNF-Î± Â serum Â concentration. Â Compared to Â the Â group Â withoutÂ vitamin Â D Â supplementation, Â group Â with Â vitamin Â D Â has Â higher Â proportion Â of Â sputum Â conversion Â in Â the Â first Â monthÂ (67% Â  Â vs. 34%, p=0,04) and second month (95% Â vs. 77%, p=0,18). Â  Â Improvement Â in Â radiographic Â findings Â inÂ the Â group with vitamin D is Â also higher in Â the Â first Â month (67% Â vs. 18%, p=0,02) and second month (76% Â  Â vs.Â 45%, Â p=0,06). Â  Â There is Â a Â significant Â difference Â in Â the Â level Â of Â 25-dihidroxyvitamin Â D between Â two Â groupsÂ (p=0,00). However, Â there Â is Â no significant difference in Â the Â level Â of TNF-Î± between two Â groups (p=0,76) afterÂ two Â months.
Ekspresi dan Aktivasi Sub Unit p65 NF-ÎºB pada Sel Mononuklear Penderita Sindrom Nefrotik Resisten Steroid Anak
Authors: Corebima Brigitta Ida RV , Subandiyah Krisni , Fitri Loeki Enggar
Published: January 2017

Resitance Â to Â steroid Â therapy Â is Â a Â poor Â prognosis Â sign Â for Â nephrotic Â syndrome, Â but Â this Â mechanism is Â stillÂ unclear. NF-ÎºB has been reported to affect the glucocorticoid at the T cell through the transrepression in Â theÂ glucocorticoid Â receptor . Â Until Â know, Â this Â study Â was Â still Â limited. Â The Â aim Â of Â this Â study Â was Â to Â show Â the Â differenceÂ expression Â and Â activation Â of Â NF-ÎºB Â p65 Â subunit Â between Â childhood Â SRNS Â and Â SSNS Â patient. Â Cross Â sectionalÂ study Â was Â conducted Â during Â January Â 2009 Â to Â December Â 2009 Â in Â the Â Saiful Anwar Â General Â Hospital Â andÂ Biomedic Â Laboratory Â Brawijaya Â University. Â Expression Â and Â activation Â of Â NF-ÎºB Â p65 Â subunit Â were Â determine Â byÂ immunochemistry Â staining Â by Â counting Â the Â amount Â of Â lymphocyte Â that Â express Â and Â activate Â NF-ÎºB Â p65 Â subunitÂ among Â 200 Â lymphocytes Â under Â light Â microscope Â with Â 1000 Â magnification. Â Results Â was Â analyzed Â byÂ independent Â t Â test Â with Â 95% Â CI Â (p<0,05) Â and Â Pearson Â correlation. Â The Â result Â of Â this Â study Â revealed Â noÂ significant Â difference Â of Â expression Â of Â NF-ÎºB p65 Â subunit Â between Â SRNS and Â SSNS Â (p=0,153), Â but Â there Â wasÂ an increase activation of NF-ÎºB p65 subunit in the SRNS patients (p=0,000).
Mastosit Perivaskuler Berperan Sebagai Vasodilator Pembuluh Darah pada Mesenterium Tikus
Authors: Ibrahim Mohamad Nur , Soleh Miftahu , Widjajanto Edi
Published: January 2017

Mastocyt Â degranulated Â can Â cause Â vasodilatation Â of Â blood Â vessels, Â but Â it Â is Â unclear Â whether Â there Â is Â aÂ relationship Â between the Â number of mastocyt with a diameter of blood vessels. This study aims to Â determineÂ the Â relationship Â between the Â number of mastocyt pherivascular and diameter of blood vessels. Â This researchÂ is Â in Â vivo Â exploratory Â research Â in Â  Â Wistar Â rat Â mesentery which was sensitated Â with ovalbumin. Â The Â diameter Â ofÂ blood Â vessels Â at Â mesentery Â preparate Â were Â measured Â microscopically Â using Â a Â micrometer Â scale Â standards. Â AtÂ large Â diameter Â of Â blood Â vessels Â  (2.860 Â Â± Â 0.348), Â the Â number Â of Â mastocyt was 12.3 Â Â± Â 2.3. Â At Â medium diameterÂ of blood vessel (1.276 Â± 0.198), the Â number of mastocyt was 4.4 Â± 0.5. Blood vessels with small diameterÂ (0.961 Â± 0.043), the Â number of mastocyt was 3.5 Â± 1.3. Calculations area on the Â microscope visual field Â wasÂ divided into Â four Â areas (counting area), there Â were zone 1, zone 2, zone 3 and zone 4. Statistical analysisÂ showed Â that Â the Â correlation Â between Â the Â number Â of Â mastocyt Â at Â  zone Â 1 Â and Â the Â diameter Â of Â blood Â vessels Â wasÂ 0,904 at 1% significance level. Â Similar to Â that Â at zone 2 (r=0,892), Â zone 3 and zone 4 (r=0,940). Â We concludeÂ there Â was Â a Â significant Â relationship Â between Â the Â number Â of Â mastocyt Â and Â the Â diameter Â of Â blood Â vessels.Â Keywords: Â mastocyt Â pherivascular, Â diameter Â of Â blood Â vessels
Laporan Kasus: Tatalaksana Anestesi Penyakit Hirschsprung dengan Sindrom Aspirasi Mekoneum dan Pneumomediastinum pada Neonatus
Authors: Yupono Karmini , Hartono Ruddi
Published: January 2017

We report Â one female Â newborn baby suffered from Â moderate asphyxia, meconium aspiration syndrome, andÂ Hirschsprung Â disease. Â The Â patient Â admitted Â in Â Neonatal Â Intensive Â Care Â Unit Â (NICU) Â and Â ventilated Â withÂ Pressure Controlled Mandatory Ventilation (PCMV) Â mode ventilator. Â Patient suffered from Â pneumodiastinumÂ and therefore Â mediastinotomy was performed. Sepsis and Necrotizing Enterocolitis (NEC) Â made the Â caseÂ more Â complicated. Â On Â further Â examination, Â abdominal Â was Â distended Â and Â perforated Â and Â therefore Â weÂ decided Â to Â perform Â exploration Â laparatomy Â and Â sigmoidoctomy . Â Anaesthesia Â management Â with Â generalÂ anaesthesia Â had Â been Â performed. Â Premedication Â was Â not Â given. Â We Â used Â oxygen Â and Â Sevofluran Â forÂ induction; Â Oxygen, Sevofluran, Atracurium, Fentanyl, Â and Â Morphin for Â maintenance; and Â Morphin for Â postÂ operative analgesic. On follow up examination, we found wound disruption and therefore relaparotomy wasÂ performed. Â T ypes Â and Â doses Â of Â anesthesia Â drugs Â were Â chosen Â according Â to Â newborn Â immature Â organ Â noticely .Â Providing adequate oxygenation, preventing hypotermi, and balancing electrolytes and acid base states stillÂ be the Â most considerations in Â management of this Â patient. Â Anesthetic management and intensive Â care of thisÂ patient Â gained Â a Â satisfying Â outcome. Â The Â prognosis Â was Â good.Â Keywords: Â Hirschsprung Â disease, Â meconium Â aspiration Â syndrome, Â pneumomediastinum, Â sepsis, Â NEC.
Korelasi Antara Transforming Growth Factor -Î’1 Monosit dengan Kebocoran Vaskuler pada Demam Berdarah Dengue
Authors: Supriatna Mohamad , Tondy Haryson , Ermin Tatty , Istanti Yusrina , RMD Kisdjamiatun
Published: January 2017

ABSTRAKSel monosit merupakan target utama infeksi virus Demam Berdarah Dengue (DBD) yang dapat memproduksi faktorÂ vasoaktif yang mempengaruhi fungsi sel endotel dan berperan dalam kebocoran vaskuler. Transforming growth factorÂ beta 1 (TGF-1) merupakan salah satu sitokin yang mempunyai banyak peran dalam patogenesis DBD. Penelitian iniÂ dilakukan untuk mengidentifikasi hubungan antara TGF-1 terhadap indeks efusi pleura (IEP), total protein, albumin danÂ hematokrit sebagai indikator kebocoran vaskular. Studi dilakukan dengan desain cross sectional pada pasien DBD usia 13-14 tahun di RS dr. Kariadi selama Juli 2005 hingga Juli 2006. Sampel pemeriksaan adalah kultur whole blood supernatantÂ culture yang diinduksi LPS pada subyek penelitian. Kadar monosit TGF-1, PEI, total protein, albumin dan hematokrit diukurÂ pada hari 0 dan 2. Data di analisis dengan uji Wilcoxon dan korelasi Spearman. Pada hari 0 dan 2 dianalisis korelasi antaraÂ kadar monosit TGF-1, PEI juga protein total, albumin dan hematokrit. Rerata kadar TGF-1 monosit pada hari ke 2Â (43.29Â±28.012 pg/ml) lebih tinggi dari hari 0 (35.27 Â± 34.642 pg/ml, p=0.09, Wilcoxon test). Pada hari 0 terdapat korelasiÂ signifikan level TGF-1 monosit dengan PEI (r=-0.31, p=0.04), tetapi tidak dengan total protein (r=0.19, p=0.2), albuminÂ (r=0.11, p=0.5 ; r=--.08, p=0.6). Pada hari kedua tidak ada korelasi signifikan antara level TGF-1 monosit level dan PEI (r=-0.2,p=0.3), total protein (r=0.2, p=0.2), albumin (r-0.2, p=0.2) dan hematokrit (r=-0.04, p=0.8). Dapat di simpulkan bahwaÂ TGF-1 mungkin berperan dalam proses inflamasi DBD melalui PEI sebagai salah satu indikator kebocoran plasma.Kata Kunci : Albumin, DBD, hematokrit, IEP, TGF-1, protein totalÎ²
Ekstrak Jinten Hitam Memperbaiki Penyempitan Jalan Nafas pada Model Mencit Asthma
Authors: Sriwahyuni Endang , Risza Faradina , Yuni Anita
Published: January 2017

ABSTRAKRemodeling jalan nafas pada asma merupakan perubahan yang bersifat ireversibel dan di tunjukkan dengan penebalanÂ epitel. Asma merupakan kondisi hipersensitif jalan nafas yang disebabkan inflamasi dan ditunjukkan dengan penyempitanÂ bronkus. Jinten hitam sudah digunakan untuk pengobatan asma sejak lama. Studi dilakukan untuk menguji efek jintenÂ hitam dalam mencegah penebalan bronkus dan membuktikan efek bronkodilator dengan peningkatan lingkar lumenÂ bronkus pada model mencit asma betina dengan eksperimental in vivo. Mencit model asma dikembangkan denganÂ memberikan sensitisasi allergen ovalbumin yang diberikan intraperitoneal dua kali dalam 3 minggu (haro 0 dan 14)Â dilakukan per inhalasi tiga kali seminggu selama 6 minggu. Ekstrak jinten hitam diberikan dalam 3 dosis (1,2g; 2,4 g and 4,8Â g/kgBB/hari) selama 9 minggu. Hasil penelitian menunjukkan bahwa pemberian jintan hitam dapat mencegah penebalanÂ epitel bronkus dengan dosisi efektif 2,4 g/Kg BB/hari dan meningkatkan lingkar lumen bronchial dengan dosis efektif 4,8Â g/KgBB/hari. Dapat disimpulkan pemberian ekstrak jintan mencegah penebalan epital bronkus dan meningkatkan lingkarÂ lumen bronchial pada mencit model asma.Kata Kunci : Asma, inflamasi, jinten hitam, lingkar lumen bronkus, penebalan epitel bronkus
Gangguan Keseimbangan Elektrolit Sesudah Kemoterapi Induksi Remisi pada Anak dengan Leukemia Limfoblastik Akut
Authors: Nugroho Susanto
Published: January 2017

ABSTRAKGangguan keseimbangan elektrolit sering di jumpai pada leukemia limfoblastik akut pada anak karena proses leukemia,Â infiltrasi organ dan kematian sel akibat efek samping obat sitotoksik. Dari berbagai gangguan tersebut, gangguanÂ keseimbangan elektrolit (hiperkalemi, hiperfosfatemi dan hipokalsemi) juga hyperuricemia dan azotemia paling seringÂ ditemukan. Meskipun tidak mengancam jiwa, gangguan ini berpotensi menyebabkan efek kardiotoksi akibat kemoterapiÂ seperti kematian mendadak karena aritmia malignant. Studi retrospektif ini dilakukan untuk mengevaluasi perubahanÂ level serum elektrolit (potasium, kalsium, dan fosfor), asam urat dan ureum pada anak dengan ALL dan menerima induksiÂ remisi kemoterapi menggunakan protokol Indonesia ALL-2006. Subjek penelitian adalah 34 anak dengan kasus baru ALLÂ yang menerima remisi induksi kemoterapi. Terdapat peningkatan signifikan level serum potasium (p=0,035) dan fosforÂ (p=0,039), sebaliknya terdapat penurunan signifikan kadar calcium serum (p=0,008). Disamping itu juga ditemukanÂ peningkatan kadar asam urat dan ureum serum tetapi tidak signifikan. Dapat disimpulkan bahwa induksi remisiÂ kemoterapi menggunakan protokol ALL 2006 dapat menyebabkan ketidakseimbangan elektrolit.Kata Kunci : A Â ketidakseimbangan elektrolit, leukemia limfoblastik akutÂ nak, kemoterapi,