bluuebunny/crossref_metadata_2025 huggingface dataset:
A Blow to the Eye—Post Traumatic Orbital Emphysema Associated With Blowout Fracture
Authors: Qundos Yousef , Thor Andreas
Published: December 2020

A case of a blowout fracture with sever post traumatic emphysema is described in this study. The clinical presentation, diagnosis and management of orbital emphysema associated with blowout fractures are discussed, and the literature reviewed. This case highlights the importance of proper diagnostic measures and necessary treatment when dealing with orbital trauma patients. The clinical examination of motility, visual acuity and pupillary affect are primarily the most important factors of deciding suitable therapy. In an emergency, time is of the essence to reduce the risk of permanent vision loss.
When Artificial Intelligence Meets Behavioural Economics
Authors: Balasubramanian Girish
Published: December 2020

Behavioural economics has its roots in the problems of rationality and optimising the expected utility, specially the empirical evidence of individuals acting against expected norms. Artificial intelligence (AI), on the other hand, is premised on the dominant idea being that because of the dispositional factors, the human being often might be akin to a disturbance to an otherwise smooth system. Thus, the intersection of both these areas is decision-making under uncertainty. Both these concepts put together have interesting implications for organisations. This article explores the impact of AI and Behavioural Economics on the human resources (HR) function of an organisation. Some of the contemporary applications of AI augmenting decision-making have been presented using the lens of the HR Value Chain. Based on these applications, implications for organisations are discussed. Despite limitations, AI, as a technology, is soon going to be embraced by the firms, leading to hybrid organisations. As a result, organisations need to redesign their processes and policies.
Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii
Authors: Ahmed Hassan M. M. , Heese Fabienne , Wimmer Ernst A.
Published: December 2020

Background

The invasive flyDrosophila suzukiihas become an established fruit pest in Europe, the USA, and South America with no effective and safe pest management. Genetic engineering enables the development of transgene-based novel genetic control strategies against insect pests and disease vectors. This, however, requires the establishment of reliable germline transformation techniques. Previous studies have shown thatD. suzukiiis amenable to transgenesis using the transposon-based vectorspiggyBacandMinos, site-specific recombination (lox/Cre), and CRISPR/Cas9 genome editing.

Results

We experienced differences in the usability ofpiggyBac-based germline transformation in different strains ofD. suzukii: we obtained no transgenic lines in a US strain, a single rare transgenic line in an Italian strain, but observed a reliable transformation rate of 2.5 to 11% in a strain from the French Alps. This difference in efficiency was confirmed by comparative examination of these three strains. In addition, we used anattPlanding site line to successfully established φC31-integrase-mediated plasmid integration at a rate of 10% and generated landing site lines with twoattPsequences to effectively perform φC31-Recombinase Mediated Cassette Exchange (φC31-RMCE) with 11% efficiency. Moreover, we isolated and used the endogenous regulatory regions ofDs nanosto express φC31 integrase maternally to generate self-docking lines for φC31-RMCE. Besides, we isolated the promoter/enhancer ofDs serendipity αto drive the heterologoustetracycline-controlled transactivator (tTA) during early embryonic development and generated a testes-specific tTA driver line using the endogenousbeta-2-tubulin(β2t) promoter/enhancer.

Conclusion

Our results provide evidence that theD. suzukiistrain AM derived from the French Alps is more suitable forpiggyBacgermline transformation than other strains. We demonstrated the feasibility of using φC31-RMCE in the cherry vinegar fly and generated a set of lines that can be used for highly efficient integration of larger constructs. The φC31-based integration will facilitate modification and stabilization of previously generated transgenic lines that carry at least oneattPsite in the transgene construction. An early embryo-specific and a spermatogenesis-specific driver line were generated for future use of the binary expression systemtet-offto engineer tissue- and stage-specific effector gene expression for genetic pest control strategies.
The hAT-family transposable element, hopper, from Bactrocera dorsalis is a functional vector for insect germline transformation
Authors: Handler Alfred M. , Schetelig Marc F.
Published: December 2020

Background

Thehopper hAT-family transposable element isolated from the Oriental fruit fly,Bactrocera dorsalis, is distantly related to both theDrosophila hoboelement and theActivatorelement from maize. The original 3120 bphopperBd-Kahelement isolated from the Kahuku wild-type strain was highly degenerate and appeared to have a mutated transposase and terminal sequences, while a second 3131 bp element,hopperBd-we, isolated from awhite eyemutant strain had an intact transposase reading frame and terminal sequences consistent with function.

Results

ThehopperBd-weelement was tested for function by its ability to mediate germline transformation in two dipteran species other thanB. dorsalis. This was achieved by creating a binary vector/helper transformation system by linking thehopperBd-wetransposase reading frame to aD. melanogaster hsp70promoter for a heat-inducible transposase helper plasmid, and creating vectors marked with theD. melanogastermini-white+orpolyubiquitin-regulated DsRed fluorescent protein markers.

Conclusions

Both vectors were successfully used to transformD. melanogaster, and the DsRed vector was also used to transform the Caribbean fruit fly,Anastrepha suspensa, indicating a wide range ofhopperfunction in dipteran species and, potentially, non-dipteran species. This vector provides a new tool for insect genetic modification for both functional genomic analysis and the control of insect populations.
Comparative genomics of Klebsiella michiganensis BD177 and related members of Klebsiella sp. reveal the symbiotic relationship with Bactrocera dorsalis
Authors: Cai Zhaohui , Guo Qiongyu , Yao Zhichao , Zheng Wenping , Xie Junfei , Bai Shuai , Zhang Hongyu
Published: December 2020

Background

Bactrocera dorsalis is a destructive polyphagous and highly invasive insect pest of tropical and subtropical species of fruit and vegetable crops. The sterile insect technique (SIT) has been used for decades to control insect pests of agricultural, veterinary, and human health importance. Irradiation of pupae in SIT can reduce the ecological fitness of the sterile insects. Our previous study has shown that a gut bacterial strain BD177 that could restore ecological fitness by promoting host food intake and metabolic activities.

Results

Using long-read sequence technologies, we assembled the complete genome of K. michiganensis BD177 strain. The complete genome of K. michiganensis BD177 comprises one circular chromosome and four plasmids with a GC content of 55.03%. The pan-genome analysis was performed on 119 genomes (strain BD177 genome and 118 out of 128 published Klebsiella sp. genomes since ten were discarded). The pan-genome includes a total of 49305 gene clusters, a small number of 858 core genes, and a high number of accessory (10566) genes. Pan-genome and average nucleotide identity (ANI) analysis showed that BD177 is more similar to the type strain K. michiganensis DSM2544, while away from the type strain K. oxytoca ATCC13182. Comparative genome analysis with 21 K. oxytoca and 12 K. michiganensis strains, identified 213 unique genes, several of them related to amino acid metabolism, metabolism of cofactors and vitamins, and xenobiotics biodegradation and metabolism in BD177 genome.

Conclusions

Phylogenomics analysis reclassified strain BD177 as a member of the species K. michiganensis. Comparative genome analysis suggested that K. michiganensis BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to B. dorsalis. The clear classification status of BD177 strain and identification of unique genetic characteristics may contribute to expanding our understanding of the symbiotic relationship of gut microbiota and B. dorsalis.
Irradiation induced inversions suppress recombination between the M locus and morphological markers in Aedes aegypti
Authors: Augustinos Antonios A. , Misbah-ul-Haq Muhammad , Carvalho Danilo O. , de la Fuente Lucia Duran , Koskinioti Panagiota , Bourtzis Kostas
Published: December 2020

Background

Aedes aegyptiis the primary vector of arthropod-borne viruses and one of the most widespread and invasive mosquito species. Due to the lack of efficient specific drugs or vaccination strategies, vector population control methods, such as the sterile insect technique, are receiving renewed interest. However, availability of a reliable genetic sexing strategy is crucial, since there is almost zero tolerance for accidentally released females. Development of genetic sexing strains through classical genetics is hindered by genetic recombination that is not suppressed in males as is the case in many Diptera. Isolation of naturally-occurring or irradiation-induced inversions can enhance the genetic stability of genetic sexing strains developed through genetically linking desirable phenotypes with the male determining region.

Results

For the induction and isolation of inversions through irradiation, 200 male pupae of the ‘BRA’ wild type strain were irradiated at 30 Gy and 100 isomale lines were set up by crossing with homozygous ‘red-eye’ (re) mutant females. Recombination betweenreand the M locus and the white (w) gene (causing a recessive white eye phenotype when mutated) and the M locus was tested in 45 and 32 lines, respectively. One inversion (Inv35) reduced recombination between bothreand the M locus, andwand the M locus, consistent with the presence of a rather extended inversion between the two morphological mutations, that includes the M locus. Another inversion (Inv5) reduced recombination only betweenwand the M locus. In search of naturally-occurring, recombination-suppressing inversions, homozygous females from the red eye and the white eye strains were crossed with seventeen and fourteen wild type strains collected worldwide, representing either recently colonized or long-established laboratory populations. Despite evidence of varying frequencies of recombination, no combination led to the elimination or substantial reduction of recombination.

Conclusion

Inducing inversions through irradiation is a feasible strategy to isolate recombination suppressors either on the M or the m chromosome forAedes aegypti. Such inversions can be incorporated in genetic sexing strains developed through classical genetics to enhance their genetic stability and support SIT or other approaches that aim to population suppression through male-delivered sterility.
Correction to: Predicting and clustering plant CLE genes with a new method developed specifically for short amino acid sequences
Authors: Zhang Zhe , Liu Lei , Kucukoglu Melis , Tian Dongdong , Larkin Robert M. , Shi Xueping , Zheng Bo
Published: December 2020

An amendment to this paper has been published and can be accessed via the original article.
Prevention of postoperative bleeding after complex pediatric cardiac surgery by early administration of fibrinogen, prothrombin complex and platelets: a prospective observational study
Authors: Dennhardt Nils , Sümpelmann Robert , Horke Alexander , Keil Oliver , Nickel Katja , Heiderich Sebastian , Boethig Dietmar , Beck Christiane E.
Published: December 2020

Background

Postoperative bleeding is a major problem in children undergoing complex pediatric cardiac surgery. The primary aim of this prospective observational study was to evaluate the effect of an institutional approach consisting of early preventive fibrinogen, prothrombin complex and platelets administration on coagulation parameters and postoperative bleeding in children. The secondary aim was to study the rate of re-intervention and postoperative transfusion, the occurrence of thrombosis, length of mechanical ventilation, ICU stay and mortality.

Methods

In fifty children (age 0–6 years) with one or more predefined risk factors for bleeding after cardiopulmonary bypass (CPB), thrombelastography (TEG) and standard coagulation parameters were measured at baseline (T1), after CPB and reversal of heparin (T2), at sternal closure (T3) and after 12 h in the ICU (T4). Clinical bleeding was evaluated by the surgeon at T2 and T3 using a numeric rating scale (NRS, 0–10).

Results

After CPB and early administration of fibrinogen, prothrombin complex and platelets, the clinical bleeding evaluation score decreased from a mean value of 6.2 ± 1.9 (NRS) at T2 to a mean value of 2.1 ± 0.8 at T3 (NRS;P <  0.001). Reaction time (R), kinetic time (K), maximum amplitude (MA) and maximum amplitude of fibrinogen (MA-fib) improved significantly (P <  0.001 for all), and MA-fib correlated significantly with the clinical bleeding evaluation (r = 0.70,P <  0.001). The administered total amount of fibrinogen (mg kg− 1) correlated significantly with weight (r = − 0.42,P = 0.002), priming volume as percentage of estimated blood volume (r = 0.30,P = 0.034), minimum CPB temperature (r = − 0.30,P = 0.033) and the change in clinical bleeding evaluation from T2 to T3 (r = 0.71,P <  0.001). The incidence of postoperative bleeding (> 10% of estimated blood volume) was 8%. No child required a surgical re-intervention, and no cases of thrombosis were observed. Hospital mortality was 0%.

Conclusion

In this observational study of children with an increased risk of bleeding after CPB, an early preventive therapy with fibrinogen, prothrombin complex and platelets guided by clinical bleeding evaluation and TEG reduced bleeding and improved TEG and standard coagulation parameters significantly, with no occurrence of thrombosis or need for re-operation.

Trial registration

German Clinical Trials RegisterDRKS00018109(retrospectively registered 27th August 2019).
Conflict, community, and collaboration: shared implementation barriers and strategies in two polio endemic countries
Authors: Owoaje Eme , Rahimi Ahmad Omid , Kalbarczyk Anna , Akinyemi Oluwaseun , Peters Michael A. , Alonge Olakunle O.
Published: December 2020

Background

Afghanistan and Nigeria are two of the three remaining polio endemic countries. While these two countries have unique sociocultural characteristics, they share major polio risk factors. This paper describes the countries’ shared contexts and highlights important lessons on implementing polio eradication activities among hard-to-reach populations relevant for future global health programs.

Methods

A grey literature review of the Global Polio Eradication Initiative (GPEI) followed by an online survey was conducted in both countries. The survey was targeted to individuals who have been involved continuously in polio eradication activities for 12 months or more since 1988. A sub-set of respondents from the survey was recruited for key-informant interviews (KII). The survey and KIIs were conducted between September 2018–April 2019. A cross-case comparison analysis was conducted to describe shared implementation challenges, strategies, and unintended consequences of polio eradication activities across these contexts.

Results

Five hundred thirteen and nine hundred twenty-one surveys were completed in Afghanistan and Nigeria respectively; 28 KIIs were conducted in Afghanistan and 29 in Nigeria. Major polio eradication activities in both countries include house-to-house campaigns, cross-border stations, outreach to mobile populations, and surveillance. Common barriers to these activities in both countries include civil unrest and conflict; competing political agendas; and vaccine refusal, fatigue, and mistrust, all of which are all bases for describing hard-to-reach populations. Both countries employed strategies to engage community leadership, political and religious groups through advocacy visits, and recruited community members to participate in program activities to address misconceptions and distrust. Recruitment of female workers has been necessary for accessing women and children in conservative communities. Synergy with other health programs has been valuable; health workers have improved knowledge of the communities they serve which is applicable to other initiatives.

Conclusions

The power of community engagement at all levels (from leadership to membership) cannot be overstated, particularly in countries facing civil unrest and insecurity. Workforce motivation, community fatigue and mistrust, political priorities, and conflict are intricately interrelated. Community needs should be holistically assessed and addressed;programs must invest in the needs of health workers who engage in these long-term health programs, particularly in unsafe areas, to alleviate demotivation and fatigue.
Allocation of intensive care resources during an infectious disease outbreak: a rapid review to inform practice
Authors: Fiest Kirsten M. , Krewulak Karla D. , Plotnikoff Kara M. , Kemp Laryssa G. , Parhar Ken Kuljit S. , Niven Daniel J. , Kortbeek John B. , Stelfox Henry T. , Parsons Leigh Jeanna
Published: December 2020

Background

The COVID-19 pandemic has placed sustained demand on health systems globally, and the capacity to provide critical care has been overwhelmed in some jurisdictions. It is unknown which triage criteria for allocation of resources perform best to inform health system decision-making. We sought to summarize and describe existing triage tools and ethical frameworks to aid healthcare decision-making during infectious disease outbreaks.

Methods

We conducted a rapid review of triage criteria and ethical frameworks for the allocation of critical care resources during epidemics and pandemics. We searched Medline, EMBASE, and SCOPUS from inception to November 3, 2020. Full-text screening and data abstraction were conducted independently and in duplicate by three reviewers. Articles were included if they were primary research, an adult critical care setting, and the framework described was related to an infectious disease outbreak. We summarized each triage tool and ethical guidelines or framework including their elements and operating characteristics using descriptive statistics. We assessed the quality of each article with applicable checklists tailored to each study design.

Results

From 11,539 unique citations, 697 full-text articles were reviewed and 83 articles were included. Fifty-nine described critical care triage protocols and 25 described ethical frameworks. Of these, four articles described both a protocol and ethical framework. Sixty articles described 52 unique triage criteria (29 algorithm-based, 23 point-based). Few algorithmic- or point-based triage protocols were good predictors of mortality with AUCs ranging from 0.51 (PMEWS) to 0.85 (admitting SOFA > 11). Most published triage protocols included the substantive values of duty to provide care, equity, stewardship and trust, and the procedural value of reason.

Conclusions

This review summarizes available triage protocols and ethical guidelines to provide decision-makers with data to help select and tailor triage tools. Given the uncertainty about how the COVID-19 pandemic will progress and any future pandemics, jurisdictions should prepare by selecting and adapting a triage tool that works best for their circumstances.
Social Internet of Things: vision, challenges, and trends
Authors: Malekshahi Rad Mozhgan , Rahmani Amir Masoud , Sahafi Amir , Nasih Qader Nooruldeen
Published: December 2020

IoT describes a new world of billions of objects that intelligently communicate and interact with each other. One of the important areas in this field is a new paradigm-Social Internet of Things (SIoT), a new concept of combining social networks with IoT. SIoT is an imitation of social networks between humans and objects. Objects like humans are considered intelligent and social. They create their social network to achieve their common goals, such as improving functionality, performance, and efficiency and satisfying their required services. Our article’s primary purpose is to present a comprehensive review article from the SIoT system to analyze and evaluate the recent works done in this area. Therefore, our study concentrated on the main components of the SIoT (Architecture, Relation Management, Trust Management, web services, and information), features, parameters, and challenges. To gather enough information for better analysis, we have reviewed the articles published between 2011 and December 2019. The strengths and weaknesses of each article are examined, and effective evaluation parameters, approaches, and the most used simulation tools in this field are discussed. For this purpose, we provide a scientific taxonomy for the final SIoT structure based on the academic contributions we have studied. Ultimately we observed that the evaluation parameters are different in each element of the SIoT ecosystem, for example for Relation Management, scalability 29% and navigability 22% are the most concentrated metrics, in Trust Management, accuracy 25%, and resiliency 25% is more important, in the web service process, time 23% and scalability 16% are the most mentioned and finally in information processing, throughput and time 25% are the most investigated factor. Also, Java-based tools like Eclipse has the most percentage in simulation tools in reviewed literature with 28%, and SWIM has 13% of usage for simulation.
Correction to: A randomised controlled feasibility study of interpersonal art psychotherapy for the treatment of aggression in people with intellectual disabilities in secure care
Authors: Hackett Simon S. , Zubala Ania , Aafjes-van Doorn Katie , Chadwick Thomas , Harrison Toni Leigh , Bourne Jane , Freeston Mark , Jahoda Andrew , Taylor John L. , Ariti Cono , McNamara Rachel , Pennington Lindsay , McColl Elaine , Kaner Eileen
Published: December 2020

An amendment to this paper has been published and can be accessed via the original article.
Are benzodiazepines effective in treating catatonia?
Authors: Carthy Elliott
Published: December 2020

Establishing an evidence base for the clinical management of catatonia is made difficult by the heterogeneous nature of the condition and the limited understanding of its pathophysiology. Benzodiazepines are a mainstay of treatment. The Cochrane review discussed identified only one eligible study (17 participants with catatonia who received either lorazepam or oxazepam), which found no difference on the single outcome measure (a 50% improvement on a visual analogue scale). This commentary discusses the findings in more detail, and considers what constitutes high-quality evidence for the acute treatment of catatonia, why there is such a paucity of randomised controlled trials (RCTs) on the topic and whether RCTs are both feasible and appropriate for the condition.
Glucose Tolerance Stages in Cystic Fibrosis Are Identified by a Unique Pattern of Defects of Beta-Cell Function
Authors: Piona Claudia , Volpi Sonia , Zusi Chiara , Mozzillo Enza , Tosco Antonella , Franzese Adriana , Raia Valeria , Boselli Maria Linda , Trombetta Maddalena , Cipolli Marco , Bonadonna Riccardo C , Maffeis Claudio
Published: December 2020

Objective

We aimed to assess the order of severity of the defects of 3 direct determinants of glucose regulation—beta-cell function, insulin clearance, and insulin sensitivity—in patients with cystic fibrosis (CF), categorized according their glucose tolerance status, including early elevation of mid-level oral glucose tolerance test (OGTT) glucose values (>140 and <200 mg/dL), referred to as AGT140.

Methods

A total of 232 CF patients aged 10 to 25 years underwent OGTT. Beta-cell function and insulin clearance were estimated by OGTT mathematical modeling and OGTT-derived biomarkers of insulin secretion and sensitivity were calculated. The association between glucometabolic variables and 5 glucose tolerance stages (normal glucose tolerance [NGT], AGT140, indeterminate glucose tolerance [INDET], impaired glucose tolerance [IGT], cystic fibrosis–related diabetes CFRD]) was assessed with a general linear model.

Results

Beta-cell function and insulin sensitivity progressively worsened across glucose tolerance stages (P < 0.001), with AGT140 patients significantly differing from NGT (all P < 0.01). AGT140 and INDET showed a degree of beta-cell dysfunction similar to IGT and CFRD, respectively (all P < 0.01). Insulin clearance was not significantly associated with glucose tolerance stages (P = 0.162). Each stage of glucose tolerance was uniquely identified by a specific combination of defects of the direct determinants of glucose regulation.

Conclusions

In CF patients, each of the 5 glucose tolerance stages shows a unique pattern of defects of the direct determinants of glucose regulation, with AGT140 patients significantly differing from NGT and being similar to IGT. These findings suggest that AGT140 should be recognized as a distinct glucose tolerance stage and that reconsideration of the grade of glucometabolic deterioration across glucose tolerance stages in CF is warranted.
Apoptotic Cell Death in Ewe Endometrium during the Oestrous Cycle
Authors: BENBIA S. , BELKHIRI Y. , YAHIA M.
Published: December 2020

We hypothesized that endometrial tissues from ewes undergo spatial and temporal changes. Thus, two regulatory events were investigated in this study: cell death (apoptosis) and cell proliferation. Uteri were obtained from healthy ewes at Batna abattoir (Algeria). Based on macroscopic observation of the ovaries and plasma progesterone, uteri were assigned to follicular, early and active luteal phases. Apoptosis and proliferation were assessed by detection of cleaved caspase-3 and Ki-67, respectively. Ki-67 and cleaved caspase-3 (CCP-3) were expressed in both phases of the oestrous cycle and all endometrium cells types [luminal epithelia (LE), superficial gland epithelia (SG) and deep gland epithelia (DG)]. Immunohistochemistry for cleaved caspase-3 revealed few or no apoptotic stained cells in all endometrium locations during the entire oestrous cycle. However, Ki-67 was significantly higher in the follicular phase than in the early and active luteal phase. Besides, expression of CCP-3 in LE was higher than in SG and DG at the follicular phase and early luteal phase. However, Ki -67 and CCP-3 levels in all endometrium cells types did not significantly change at active luteal phase. Therefore, it is concluded that apoptosis and proliferation were occurred in ewe endometrium in a cyclic pattern and under the influence of the endocrine profile.
Biochemical composition and biometric parameters of Mytilus galloprovincialis from Boka Kotorska Bay in Southern Adriatic Sea
Authors: GRKOVIĆ N. , TEODOROVIĆ V. , DJORDJEVIĆ V. , KARABASIL N. , STAJKOVIĆ S. , VASILEV D. , ZUBER BOGDANOVIĆ I. , JANKOVIĆ S. , VELEBIT B. , DIMITRIJEVIĆ M.
Published: December 2020

This study reports, for the first time, different physico-chemical analyses, of Mediterranean mussels (Mytilus galloprovincialis) from harvesting areas in the Montenegro coast of the Adriatic Sea, in order to evaluate the influence of origin on different parameters and assessed the quality of shellfish grown in this area. The Boka Kotorska Bay is situated in Montenegro, in the south-eastern part of the Adriatic Sea The mussel samples were collected at the same time in the spring of 2019 at six locations in Boka Kotorska Bay, Montenegro: Kotor and Tivat Bays. Biometric parameters, percentage of meat, condition index, proximate composition, sensory evaluation and lipid profiles of mussels were studied. The concentrations of some micro and macro elements and heavy metals in mussels, were also analyzed. Significant differences were found between mussels from different locations. Mean biometric parameters of mussels grown in Sv.Nedjelja, were considerably higher than in mussels grown elsewhere.. Protein, lipid, ash and glycogen content were varied from 7.80 to 10.26%; 1.36 to 2.18%; 1.73 to 3.34% and 12.81 to 15.38%, respectively. Gas chromatographic analysis showed that polyunsaturated fatty acids (PUFAs) were dominant lipids in mussels (37.56 to 41.08%), followed by monounsaturated (MUFAs) (30.52 to 38.31%) and saturated (SFAs) (21.89 to 29.45%) fatty acids. Fatty acid profiles were investigated and revealed high contents of n-3 PUFAs and high n-3/n-6 ratios in all mussels from Montenegro In the mussel samples high concentrations of K, Mg, Ca, and Na, and much lower concentrations of Fe, Mn, Zn, and Cu were found. Some of toxic elements (As, Pb, Hg and Cd) were determined also.. The qualitative sensory assessment showed that all mussels were acceptable. All mussels in the current study achieved scores of 3 or more out of 5 in the qualitative category. Data on biochemical composition and quality indices of the mussels cultured in the Boka Kotorska Bay demonstrated that these products could be accepted well by consumers and could compete with other currently available mussels from other locations in Adriatic Sea.
Effect of trans-resveratrol or ubiquinol supplementation on the sperm morphology of CD-1 mice subjected to forced swimming
Authors: VAAMONDE Δ. , SÁNCHEZ DE MEDINA A. , CORTÉS Β. , DÍAZ Α. , RODRÍGUEZ Ι.
Published: December 2020

This study was undertaken to assess the effect of a three-minute forced swimming protocol for 50 days, with and without antioxidant administration on sperm morphology in CD-1 mice. Seventy-five mice were randomly allocated to one of the following five groups: no exercise (control group; CG), swimming without antioxidant administration (EX), swimming with trans-resveratrol administration (EX-Resv), swimming with ubiquinol and excipient administration (Kaneka´s ubiquinol) (EX-Ubiq), and swimming with just only the excipient for Kaneka´s ubiquinol administration (EX-Excp). The EX group showed that 53.03±4.83% of sperm had abnormal morphology, with significant differences with regards to CG (46.47±10.57%) (p<0.05). The number of sperm with abnormal morphology decreased in all groups treated with either antioxidants or with excipient; this was most noticeable in EX-Ubiq (p<0.05). The percentage of midpiece and tail, as well as multiple anomalies were greater in EX than in CG (p<0.05). While both antioxidants, as well as the excipient, decreased midpiece and head anomalies, only trans-resveratrol and ubiquinol had an effect on multiple anomalies. Furthermore, only trans-resveratrol had an effect upon tail anomalies. The imposed exercise caused alterations in CD-1 mice sperm morphology, and antioxidant treatment seems suitable to decrease morphological anomalies. Both trans- resveratrol and ubiquinol were effective in decreasing simple as well as multiple sperm anomalies.
Squamous Cell Carcinoma (SCC) in Brown meagre (Sciaena umbra Linnaeus, 1758), a new candidate species for aquaculture in Mediterranean
Authors: KOLYGAS M.N. , LAMPOU E. , DOUKAS D. , TONTIS D. , PAPPAS I.S. , GOURZIOTI E. , BAKOPOULOS V. , KAKAVA E. , ATHANASSOPOULOU F.
Published: December 2020

A case of epithelial tissue tumors on the body of two adult Brown meagres (Sciaena umbra Linnaeus, 1758) is reported. Brown Meagre population of 20.000 was imported to sea cages on September of 2003 at 3g, to check the potential use of this species as a new candidate in Mediterranean aquaculture. The population had been kept for 8 years to serve as a genetic pool for further zootechnical investigation. The main pathology observed after 8 years in the sea cages were mainly ocular lesions (cataract) possibly due to natural aging and/or long culture period under high natural light intensity (UV radiation) and/or net injuries at a percentage of 1,32% of total cultured population. Apart from that, on two fish, reddish- white tumorous growths were evident on skin, macroscopically. Histologically, the timorous growths were diagnosed as Squamous cell carcinoma. Numerous rounded pearls and nests of epidermal proliferated cells were evident, some of which had a non-cellular center. There were also foci of necrosis and irregular deposits of keratin within the tumor tissue. Special stains (Ayoub-Shklar, Haematoxylin-Eosin & Schiff’s Periodic Acid) were applied to the tissue for comparison. To our knowledge this is the first presented report of SCC in this species.
Determination of the ciprofloxacin-resistant Escherichia coli isolated from chicken meat in Turkey
Authors: SAHIN S.
Published: December 2020

In this study, the occurrence of the ciprofloxacin-resistant (CR) Escherichia coli in chicken meat was determined, and their clonal relations were investigated by using pulsed-field gel electrophoresis (PFGE). Antimicrobial resistance patterns of E. coli isolates were determined by using disc diffusion assay, and minimum inhibitory concentration of ciprofloxacin was determined by E-test. Plasmid-mediated quinolone resistance (PMQR) and extended spectrum beta-lactamase (ESBL) resistance genes were also screened through polymerase chain reactions. Sixty chicken meat samples were collected from different supermarkets and butchers in Sivas, Turkey. CR E. coli strains were determined in 59 (98.3%) chicken meat samples. By analyzing PFGE fingerprint data, 34 different pulsotypes were determined. All E. coli strains were found to be resistant to nalidixic acid, enrofloxacin, and norfloxacin. In addition, isolates were resistant to levofloxacin (40.7%), ampicillin (94.9%), trimethoprim-sulfamethoxazole (76.3%), tetracycline (69.5%), and chloramphenicol (44.1%). However, isolates were susceptible to imipenem and colistin. In this study, 81.4% of CR E. coli isolates were observed to have a multidrug-resistant profile, which is defined as resistance to three or more classes of antibiotics. Through phenotypic confirmation tests, five isolates (8.3%) were determined to be ESBL-producing. The PMQR genes were not determined in any of the isolates. Two isolates (3.4%) possessed the blaCTX-M and blaCMY-2 genes, and 40 isolates (67.8%) had the blaTEM gene. Taken together, retail raw chicken meat is highly contaminated with CR E. coli. However, these isolates are not found to be carriers of the PMQR genes, indicating a low public health problem.
The importance and role of job creation at local level under the economic crisis conditions. Study and implementation of a Local Action Plan for Employment (LAPE) in the Municipality of Volos
Authors: Ntasios Nikos
Published: December 2020

The purpose of this study is to explore the possibilities of decentralization and departure from social policies, with an emphasis on employment policies, in the framework of an integrated strategy to tackle rising unemployment. In this context, this paper attempts to promote an integrated strategy for the "localization" of employment policies linked to productive reconstruction at micro-region level (i.e. Municipality of Volos) through the design and implementation of a Local Action Plan for Employment The study exemplifies the basic design requirement of an LAPE by exploring through a “sectoral research” the potential for job supply.
Ιωάννης Βαβούρας και Χαρά Βαβούρα, Οικονοµική Πολιτική
Authors: Συρμαλή Μαρία Ελένη
Published: December 2020

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Incidence of acute pulmonary embolism among patients hospitalized with COVID-19: a systematic review and meta-analysis
Authors: Munner Mohamed S. , Ritchie Charles A. , Elkhidir Ibrahim H. , Mohammadat Doaa T. , Ahmed Hussein J. , Altayeb Khalid A. , Yassin Rawan Z. , Hassan Riyan M. , Hamad Saada A. , Nimir Mohammed , Hamid Osman S. , Johnson Margaret M. , Narula Tathagat , Erben Young , Tawk Rabih G. , Miller David A. , Gupta Vivek , Devcic Zlatko , Freeman William D. , Toskich Beau B.
Published: December 2020

Background: Coronavirus disease 2019 (COVID-19) is a global pandemic, which is associated with venous thromboembolism and pulmonary embolism (PE). This study aimed to estimate the pooled incidence of PE among patients hospitalized with COVID-19 within the published literature.

Methods: This systematic review and meta-analysis was performed according to PRISMA guidelines. An electronic search using MEDLINE /PubMed, ScienceDirect, Cochrane, and OpenGray databases was conducted May 19th, 2020. Search terms included "COVID 19", "SARS-CoV-2”, "coronavirus disease 2019", "2019-nCoV", "Wuhan coronavirus", “Pulmonary embolism”, "pulmonary thromboembolism", “Pulmonary embol*”, “pulmonary thrombo*” and “PE”. Eligible studies included sufficient data to calculate the incidence of PE diagnosed during hospitalization in patients with COVID-19. Case reports were excluded. Quality was assessed using the Newcastle-Ottawa scale (observational cohort and case-control), AXIS tool (cross-sectional), and quality assessment tool (case series). Demographics and PE incidence data were extracted from the included studies and analyzed with R language. The pooled incidence of PE in patients hospitalized with COVID-19 was calculated.

Results: The database search identified 128 records. Ten observational studies were eligible and were included in the meta-analysis with a total of 1722 patients (mean age= 63.36). The pooled PE incidence in patients hospitalized with COVID-19 was 17% (95% CI: 0.1-0.26). There was a high degree of study heterogeneity (I2 = 94%, p<0.01).

Conclusion: The pooled PE incidence in patients hospitalized with COVID-19 is 17%. This increased incidence is greater than that previously reported in the general population of non-COVID-19. Attention and further investigation of this risk is warranted.
The efficacy of acupuncture as a complementary treatment for pain and anxiety after breast cancer surgery: Study protocol for a pragmatic randomized control trial
Authors: López de San Román-Fernández Cristina , Fernández-Jané Carles , Cusidó Maite , Costa-Tutusaus Lluís
Published: December 2020

Background: Breast cancer is one of the most prevalent cancers worldwide. Fortunately, survival has improved in recent years thanks to its early detection and curative treatments such as mastectomy. However, this medical procedure is associated with a range of unwanted effects such as postoperative pain and anxiety. Some studies have reported that acupuncture could be an effective treatment to control these types of symptoms, although only few studies have been conducted on women undergoing mastectomy. 

Methods:  This is a pragmatic randomized controlled trial with blind assessors. The study will be conducted in the Breast Unit of Hospital Universitario Sagrado Corazon of Barcelona (Spain).  A sample of 40 women will be recruited and randomized to receive acupuncture treatment in addition to standard care procedures, or standard care procedures alone. The main outcome, pain, will be assessed after the surgical intervention and 4, 10 and 30 days later using the numerical rating scale. Secondary outcomes include anxiety, use of analgesics, nausea, adverse effects, and surgical complications.

Discussion: Acupuncture is a low-cost non-pharmacological strategy. This study will help to clarify its possible role in controlling post-mastectomy adverse effects.

Trial registration: ClinicalTrials.gov NCT04608175 29/10/2020
Egg parasitoids of the cassava hornworm (Erinnyis spp.) associated to cassava in the Pará State, Brazil
Authors: Noronha Aloyséia Cristina da Silva , Blanco Dimison Garcia , Costa Valmir Antonio , Querino Ranyse Barbosa , Araújo Dênmora Gomes de , Johnson Norman F.
Published: December 2020

The aim of this study was to assess the occurrence and identify the egg-parasitoid species of the cassava hornworm (Erinnyis spp.) in cassava plants (Manihot esculenta Crantz - Euphorbiaceae), facilitating their usage in the biological control as a management strategy in the Pará State. During February to December of 2016, cassava hornworm eggs were collected in the Cassava Germplasm Bank area of the Embrapa Amazônia Oriental, located at the city of Belém, State of Pará. In the laboratory, eggs were separated in viable and parasitized and were daily observed until the hatching of the caterpillars and emergence of the parasitoids. The caterpillars were reared until reaching the adult stage and the sex determined at the pupae stage. A total of 482 eggs were collected, with a higher occurrence at March, and 244 caterpillars were obtained with sex ratio of 0.52 with 191 specimens reaching adult stage. Among these adults, 95.81% were Erinnyis ello (Linnaeus) and 4.19% Erinnyis alope (Drury). A total of 1,087 parasitoids were obtained from 131 eggs and belong to four Hymenoptera families: Platygastridae (Telenomus dilophonotae Cameron), Eulophidae (Chrysonotomyia sp. aff. serjaniae), Trichogrammatidae (Trichogramma marandobai Brun, Moraes & Soares) and Encyrtidae (Ooencyrtus sp.). The Erinnyis spp. eggs were mostly parasitized by T. dilophonotae, which was classified along with T. marandobai as constant. The occurrence of these natural enemies highlights the potential for natural biological control against Erinnyis spp.
Ensino de língua estrangeira em contexto digital: uma nova oportunidade de aprendizado para professores e alunos
Authors: Maria Ramos Oliveira Adriana , Maria Ferreira do Nascimento Evânia , Ribeiro Nunes Gabriela , Santos Brigida Marcela
Published: December 2020

Adriana Maria Ramos Oliveira possui graduação e licenciatura em Letras (Português-Espanhol) pela Universidade Federal do Rio de Janeiro (1996), especialização como professora de Lengua y Literaturas Españolas pela Agencia Española de Cooperación Internacional – AECI (1997), mestrado em Letras Neolatinas pela Universidade Federal do Rio de Janeiro (2000), magíster em Alta Especialización en Filología Hispánica pelo Consejo Superior de Investigaciones Científicas – CSIC (2002), Doutorado em Língua Espanhola e Linguística Aplicada – UNED (2002) e Doutorado em Estudos da Linguagem – UFF (2019). É professora colaboradora nos cursos de Pós-Graduação da Universidade Estácio de Sá, professora do Instituto Cervantes e professora do Ensino Básico Técnico e Tecnológico do Centro Federal de Educação Tecnológica Celso Suckow da Fonseca – CEFET/RJ no Bacharelado em Línguas Estrangeiras Aplicadas às Negociações Internacionais (LEANI). Coordena o LEANI TRAD (Laboratório de Tradução do Bacharelado em LEANI) e tem experiência na área de Letras, com ênfase em Línguas Estrangeiras Modernas (Espanhol), Formação de Professores, Tradução e Novas Tecnologias Aplicadas ao Ensino de Idiomas.Em entrevista concedida via e-mail, Adriana Maria Ramos Oliveira discute o uso de ferramentas digitais nas aulas de língua estrangeira.
Expressive component of the interpreter’s emotional response in consecutive interpreting
Authors:   Poltava State Agrarian Academy, Reva I. A.
Published: December 2020

The article highlights the main issues of consecutive bilateral interpretation from the point of view psycholinguistics and translation studies. The daily translation is a well-established, ordinary, new terminology, which is translated according to a one-sided (sequential) model. The lexical stock of the translator is characterized as a complex system of language units, where the important place is occupied by functional words that serve as connecting or expressive elements in semantic units-phrases and sentences. The communicative process, which is performed according to its status of the language level has analyzed. Varieties of the speaker’s language have distinguished in translation practice. The concept of oral and consecutive translation has substantiated. The sign system of communication has developed. Praxemics has considered as one of the areas of psychology in the study of nonverbal communication, that is covering the doctrine of touch, kinesics, time structure. The physiological basis of the translator’s perception has revealed. The translator is a person who constantly interacts with the world around him. Any act of such interaction is based on the sensory representation of its immediate environment, which includes general orientation, assessment of the location relevant objects, their physical properties, situational significance, behavioral, symbolic or aesthetic content. The concept of „primary information” has formulated, which serves as a source for the emergence and functioning of higher forms of mental activity that is going beyond the immediate given and provide regulation of various oriented, cognitive, practical activities (locomotion, problem solving, social communication or labor operations etc.).
Verbal realization of „FAILURE” and „НЕВДАЧА” concepts in contemporary American and Ukrainian fiction pieces: perception-cognitive and regulatory aspects
Authors:   Vasyl’ Stus Donetsk National University, Kalinichenko V. I.
Published: December 2020

The article describes the features of the perception-cognitive and regulation macrostructure components of the FAILURE and НЕВДАЧА concepts in the epidigmatic plane on the basis of the text pieces selected from modern American and Ukrainian fiction. The analysis of these components of the concepts under consideration is carried out in the framework of the semantic cognitive approach by performing the cognitive interpretation procedure of the text pieces, the results of which have been used for formulating semantic cognitive features that constitute the perception-cognitive and regulation layers of the concept. The specificity of the regulatory aspect of understanding the essence of failure by the bearers of American and Ukrainian linguistic consciousness is clarified due to the semantic cognitive features that structure the regulatory zone of the concept macrostructure and update the strategies of the value and target block of the individual in the context of failure. It has been found that in the epidigmatic plane, in the process of conceptualizing failure by American and Ukrainian speakers, perceptual receptors dominate cognitive-mental thought projections. The visual analyzer is the most effective for the bearers of the American and Ukrainian linguistic consciousness in the context of sensory reflection of the failure category, the sound, taste, smell and tactile receptors are less effective. Cognitive subcomponents of the FAILURE and НЕВДАЧА concepts are structured due to vital and floromorphic metaphorical images; zoomorphic and aeromorphic images for the FAILURE concept as well as religious, aquamorphic and subject images for the НЕВДАЧА concept are considered nationally specific. The regulation zone of the FAILURE concept is significantly larger than the similar НЕВДАЧА concept zone, so for Ukrainian speakers rules and guidelines that help regulate areas covering the conceptual nature of failure are more important in understanding the failed outcome of action than for American ones.