bluuebunny/crossref_metadata_2025 huggingface dataset:
Redefining Benggang Management: A Novel Integration of Soil Erosion and Disaster Risk Assessments
Authors: Yan Xiqin , Geng Shoubao , Jiang Hao , Sun Zhongyu , Wang Nan , Zhang Shijie , Yang Long , Wen Meili
Published: May 2024

In the granite regions of southern China, benggang poses a substantial threat to the ecological environment due to significant soil erosion. This phenomenon also imposes constraints on economic development, necessitating substantial investments in restoration efforts in recent decades. Despite these efforts, there remains a notable gap in comprehensive risk assessment that integrates both the erosion risk and disaster risk associated with benggang. This study focuses on a representative benggang area in Wuhua County, Guangdong province, employing transformer methods and high-resolution imagery to map the spatial pattern of the benggang. The integrated risk of benggang was assessed by combining soil-erosion risk and disaster risk, and cultivated land, residential land, and water bodies were identified as key disaster-affected entities. The machine-learning Segformer model demonstrated high precision, achieving an Intersection over Union (IoU) of 93.17% and an accuracy (Acc) of 96.73%. While the number of large benggang is relatively small, it constitutes the largest area proportion (65.10%); the number of small benggang is more significant (62.40%) despite a smaller area proportion. Prioritization for benggang management is categorized into high, medium, and low priority, accounting for 17.98%, 48.34%, and 33.69%, respectively. These priorities cover areas of 30.27%, 42.40%, and 27.33%, respectively. The findings of this study, which offer benggang management priorities, align with the nature-based solutions approach. Emphasizing the importance of considering costs and benefits comprehensively when formulating treatment plans, this approach contributes to sustainable solutions for addressing the challenges posed by benggang.
Application of Machine Learning Techniques for the Diagnosis of Obstructive Sleep Apnea/Hypopnea Syndrome
Authors: Bedoya Oscar , Rodríguez Santiago , Muñoz Jenny Patricia , Agudelo Jared
Published: May 2024

Obstructive sleep apnea/hypopnea syndrome (OSAHS) is a condition linked to severe cardiovascular and neuropsychological consequences, characterized by recurrent episodes of partial or complete upper airway obstruction during sleep, leading to compromised ventilation, hypoxemia, and micro-arousals. Polysomnography (PSG) serves as the gold standard for confirming OSAHS, yet its extended duration, high cost, and limited availability pose significant challenges. In this paper, we employ a range of machine learning techniques, including Neural Networks, Decision Trees, Random Forests, and Extra Trees, for OSAHS diagnosis. This approach aims to achieve a diagnostic process that is not only more accessible but also more efficient. The dataset utilized in this study consists of records from 601 adults assessed between 2014 and 2016 at a specialized sleep medical center in Colombia. This research underscores the efficacy of ensemble methods, specifically Random Forests and Extra Trees, achieving an area under the Receiver Operating Characteristic (ROC) curve of 89.2% and 89.6%, respectively. Additionally, a web application has been devised, integrating the optimal model, empowering qualified medical practitioners to make informed decisions through patient registration, an input of 18 variables, and the utilization of the Random Forests model for OSAHS screening.
Ruby Rich’s Dream Library: Feminist Memory-Keeping as an Archive of Affective Mnemonic Practices
Authors: Crozier-De Rosa Sharon
Published: May 2024

In the so-called West, feminist activists and scholars have long been traumatised by the erasure of their histories via dominant patriarchal narratives, which has served as an impediment to the intergenerational transmission of feminist knowledge. Recently, while acknowledging the very real and ongoing impact of this historical omission, some feminists have issued a call to turn away from a narrative of women’s history as ‘serial forgetting’ and towards an acknowledgement of the affirmative capacity of feminist remembering. At the same time, memory theorist Ann Rigney has advocated for a ‘positive turn’ in memory studies, away from what she perceives to be the field’s gravitation towards trauma and instead towards an analysis of life’s positive legacies. In this article, I combine both approaches to investigate one feminist memory-keeper’s archive, analysing what it reveals about ‘the mechanisms by which positive attachments are transmitted across space and time’. Throughout her life, little-known ‘between-the-waves’ Australian feminist Ruby Rich (1888–1988) performed multiple intersecting activist activities. While she created feminist memories through her work for various political organisations, she also collected, stored and transmitted feminist memories through her campaign for a dedicated space for women’s collections in the National Library of Australia. Propelled by fear of loss and inspired by hope for remembering, Rich constructed a brand of archival activism that was both educational and emotional. In this paper, I examine the strategies Rich employed to try to realise her dream of effecting intellectual and affective bonds between future feminists and their predecessors.
Effects of Cryogenic- and Cool-Assisted Burnishing on the Surface Integrity and Operating Behavior of Metal Components: A Review and Perspectives
Authors: Maximov Jordan , Duncheva Galya
Published: May 2024

When placed under cryogenic temperatures (below −180 °C), metallic materials undergo structural changes that can improve their service life. This process, known as cryogenic treatment (CrT), has received extensive research attention over the past five decades. CrT can be applied as either an autonomous process (for steels and non-ferrous alloys, tool materials, and finished products) or as an assisting process for conventional metalworking. Cryogenic impacts and conventional machining or static surface cold working (SCW) can also be performed simultaneously in hybrid processes. The static SCW, known as burnishing, is a widely used environmentally friendly finishing process that achieves high-quality surfaces of metal components. The present review is dedicated to the portion of the hybrid processes in which burnishing under cryogenic conditions is carried out from the viewpoint of surface engineering, namely, finishing–surface integrity (SI)–operational behavior. Analyzes and summaries of the effects of cryogenic-assisted (CrA) burnishing on SI and the operational behavior of the investigated materials are made, and perspectives for future research are proposed.
A Comprehensive Summary of the Application of Machine Learning Techniques for CO2-Enhanced Oil Recovery Projects
Authors: Du Xuejia , Salasakar Sameer , Thakur Ganesh
Published: May 2024

This paper focuses on the current application of machine learning (ML) in enhanced oil recovery (EOR) through CO2 injection, which exhibits promising economic and environmental benefits for climate-change mitigation strategies. Our comprehensive review explores the diverse use cases of ML techniques in CO2-EOR, including aspects such as minimum miscible pressure (MMP) prediction, well location optimization, oil production and recovery factor prediction, multi-objective optimization, Pressure–Volume–Temperature (PVT) property estimation, Water Alternating Gas (WAG) analysis, and CO2-foam EOR, from 101 reviewed papers. We catalog relative information, including the input parameters, objectives, data sources, train/test/validate information, results, evaluation, and rating score for each area based on criteria such as data quality, ML-building process, and the analysis of results. We also briefly summarized the benefits and limitations of ML methods in petroleum industry applications. Our detailed and extensive study could serve as an invaluable reference for employing ML techniques in the petroleum industry. Based on the review, we found that ML techniques offer great potential in solving problems in the majority of CO2-EOR areas involving prediction and regression. With the generation of massive amounts of data in the everyday oil and gas industry, machine learning techniques can provide efficient and reliable preliminary results for the industry.
Analyzing Curvature Properties and Geometric Solitons of the Twisted Sasaki Metric on the Tangent Bundle over a Statistical Manifold
Authors: Yan Lixu , Li Yanlin , Bilen Lokman , Gezer Aydın
Published: May 2024

Let (M,∇,g) be a statistical manifold and TM be its tangent bundle endowed with a twisted Sasaki metric G. This paper serves two primary objectives. The first objective is to investigate the curvature properties of the tangent bundle TM. The second objective is to explore conformal vector fields and Ricci, Yamabe, and gradient Ricci–Yamabe solitons on the tangent bundle TM according to the twisted Sasaki metric G.
Treatment Effects of Switching to Faricimab in Eyes with Diabetic Macular Edema Refractory to Aflibercept
Authors: Tatsumi Tomoaki , Kaiho Tomomi , Iwase Takehito , Miura Gen , Shimizu Daisuke , Niizawa Tomohiro , Ozawa Yoshihito , Arai Miyuki , Oshitari Toshiyuki , Takatsuna Yoko , Baba Takayuki
Published: May 2024

Background and Objectives: Faricimab is a vascular endothelial growth factor A and angiopoietin-2 bispecific antibody. It is a novel therapeutic approach distinct from previous anti-vascular endothelial growth factor agents. This study aimed to evaluate the efficacy of switching from aflibercept to faricimab in the treatment of diabetic macular edema (DME) refractory to aflibercept, with a specific focus on the resolution of macular edema. Materials and Methods: The medical records of 29 eyes of 21 patients with DME that were refractory to intravitreal injections of aflibercept (IVAs) and who had completed the clinical follow-up of at least four intravitreal injections of faricimab (IVFs) were reviewed. The central retinal thickness (CRT), best-corrected visual acuity (BCVA), and the mean period (weeks) until the next injection were measured after the second-to-last IVA, first-to-last IVA, last IVA, and first to fourth IVFs following the transition to IVF. Results: The mean time from the first IVF to the assessment of effectiveness was significantly shorter than the time to the last IVA; however, no significant difference was found in the time from the second, third, and fourth IVFs to the assessment. The mean CRTs after the first and second IVFs were not significantly different from the CRT after the last IVA, but the mean CRT after the third and fourth IVFs was significantly thinner than that after the last IVA (p = 0.0025 and p = 0.0076, respectively). The mean BCVAs after the third and fourth IVFs significantly improved compared with that after the last IVA (p = 0.0050 and p = 0.0052, respectively). Conclusions: When switching the treatment to IVF for eyes with IVA-resistant DME, better treatment outcomes are achieved if IVF is performed three or more times.
The Assessment of the Long-Term Impact of Radiotherapy on Biophysical Skin Properties in Patients after Head and Neck Cancer
Authors: Pazdrowski Jakub , Polańska Adriana , Kaźmierska Joanna , Kowalczyk Michał J. , Szewczyk Mateusz , Niewinski Patryk , Golusiński Wojciech , Dańczak-Pazdrowska Aleksandra
Published: May 2024

Background and Objectives: Chronic radiotherapy-induced skin injury (cRISI) is an irreversible and progressive condition that can significantly impact a patient’s quality of life. Despite the limited literature available on the assessment of the epidermal barrier in cRISI, there is a consensus that appropriate skincare, including the use of emollients, is the primary therapeutic approach for this group of patients. The aim of this study was to evaluate the biophysical properties of the skin during the late period (at least 90 days) following radiation therapy (RT) for head and neck cancer. Materials and Methods: This was a single-center prospective non-randomized study. It involved the analysis of 16 adult patients with head and neck cancer who underwent RT at the Greater Poland Cancer Center, along with 15 healthy volunteers. The study and control groups were matched for gender and age (p = 0.51). Clinical assessment, based on the LENT-SOMA scale, was conducted for all patients. Evaluation of the skin’s biophysical properties included: an analysis of transepidermal water loss (TEWL), stratum corneum hydration (SCH), and skin visualization using high-frequency ultrasonography (HF-USG). Results: A significantly higher TEWL was observed in the irradiated area compared to the control area in the study group (p = 0.004). However, there was no statistically significant difference in SCH (p = 0.073). Additionally, no significant difference was observed in the values of TEWL and SCH in the irradiated area between the group of patients with and without clinically obvious RISI (p = 0.192 and p = 0.415, respectively). The skin thickness of the irradiated area, assessed by HF-USG, did not differ significantly from the skin thickness of the control area (p = 0.638). Furthermore, no difference in skin thickness was observed in patients with clinical features of cRISI in the irradiated and control areas (p = 0.345). The mean time after RT was 6.1 years. Conclusions: This study marks the first demonstration of epidermal barrier damage in patients in the long term following RT for head and neck cancer. The impairment of the epidermal barrier was observed independently of evident cRISI features. This observation underscores the necessity to recommend appropriate skin care, including the use of emollients, for all patients following RT. We also suggest that HF-USG examination is generally inconclusive in determining the degree of skin damage in the late period after RT.
Association between Sarcopenia and Balance in Patients Undergoing Inpatient Rehabilitation after Hip Fractures: A Retrospective Cohort Study
Authors: Ishimoto Ryu , Mutsuzaki Hirotaka , Shimizu Yukiyo , Takeuchi Ryoko , Matsumoto Shuji , Hada Yasushi
Published: May 2024

Background and Objectives: Sarcopenia is characterized by a decline in skeletal muscle mass, strength, and function and is associated with advancing age. This condition has been suggested as a factor that negatively influences the functional outcomes of patients with hip fractures. However, the association between sarcopenia and balance impairment in patients undergoing inpatient rehabilitation after hip fractures remains unclear. In this retrospective cohort study, we aimed to investigate the impact of sarcopenia on balance outcomes in patients undergoing inpatient rehabilitation following hip fractures. Materials and Methods: Baseline sarcopenia was diagnosed using skeletal muscle mass index and handgrip strength, with cut-off values recommended by the Asian Working Group for Sarcopenia. The primary outcome was balance, which was assessed using the Berg Balance Scale (BBS) at the time of discharge. A multiple linear regression model analyzed the association between sarcopenia and balance. The model was adjusted for age, sex, comorbidities, and cognitive function. Results: Among the 62 patients (mean age: 78.2; sex: 75.8% women), 24.2% had sarcopenia. Patients with sarcopenia had significantly lower BBS scores than did those without sarcopenia (41 vs. 49 points, p = 0.004). Multiple linear regression analysis revealed that baseline sarcopenia was independently associated with BBS scores at discharge (β = −0.282, p = 0.038). Conclusions: Following inpatient rehabilitation, patients with baseline sarcopenia had inferior balance outcomes than did those without sarcopenia at discharge. Sarcopenia should be assessed on admission to consider and provide additional care for those with a higher risk of poor functional outcomes. More studies are needed to investigate the association between sarcopenia and functional outcomes, examine the impact of sarcopenia treatment on these outcomes, and reduce the risk of recurrent falls and fractures in patients with hip fractures.
Health Care Utilization for Common Shoulder Disorders: Analysis of the 2010–2019 National Patient Sample Data from the Health Insurance Review and Assessment Service in Korea
Authors: Kang Jin-Young , Kim Doori , Kim Huijun , Ha In-Hyuk , Lee Yoon Jae
Published: May 2024

Background and Objective: The aim of this study was to analyze trends in surgical and non-surgical service utilization for common shoulder disorders in Korea from 2010 to 2019. Methods and Materials: This retrospective, cross-sectional, descriptive study utilized National Patient Sample data from the Health Insurance and Review Assessment Service (HIRA) of Korea. These data constitute a 2% sample out of the entire Korean population and include data for a variety of parameters instrumental for health care research. Patients with at least one medical service use for rotator cuff syndrome or tear, impingement syndrome, or adhesive capsulitis between January 2010 and December 2019 were included. Trends in healthcare utilization by disorder type, patient demographics, seasonal service use, and treatment details were examined. Results: There was an upward trend in the total number of patients and costs for shoulder disorders, from 35,798 patients and USD 5,485,196 in 2010 to 42,558 and USD 11,522,543 in 2019, respectively. The number of patients aged ≥60 and hospital visits increased. March had the highest number of claims. Physical therapy was the most common non-surgical procedure, while nerve block claims more than doubled. Opioid prescription rates also tripled. Surgical treatments were dominated by shoulder rotator cuff repair and acromioplasty. Conclusions: There was a significant increase in healthcare utilization for shoulder disorders, marked by rising costs and patient numbers. The use of nerve blocks and opioids notably increased. These data are valuable for clinicians, researchers, and policymakers.
Assessment of CAD/CAM Fabrication Technologies for Post and Core Restorations—A Narrative Review
Authors: Dimitrova Mariya , Vlahova Angelina , Kazakova Rada
Published: May 2024

The primary objective of this study is to conduct a comprehensive review of the existing literature that discusses research on post and core restorations, covering aspects such as their composition, manufacturing methods, and clinical effectiveness. The methodology employed in this review encompasses the implementation of a well-defined search strategy, the establishment of criteria for inclusion and exclusion, and the selection of relevant studies to summarize their findings. To gather relevant literature published between 1993 and 2023, the research team conducted separate searches on PubMed, Web of Science, and Embase databases. In total, 168 titles were initially retrieved from these electronic databases. By applying the predefined exclusion criteria, the researchers identified 73 articles that specifically address the conventional and computer-aided design/computer-aided manufacturing (CAD/CAM) technologies employed in post and core restorations. These treatments are commonly employed to restore teeth that have received endodontic therapy and subsequently experienced loss of dental structure. The development of computerized technology for the creation of customized posts and cores has emerged as a straightforward and efficient alternative to traditional methods. The review synthesizes papers discussing the techniques and materials involved in CAD/CAM-based construction of post and cores. It explores strategies for restoring endodontically treated teeth, highlighting both direct and indirect approaches. Commonly mentioned materials include zirconia, composite resin, and hybrid ceramics. Despite the limited literature on CAD/CAM post and core procedures, the review emphasizes the necessity of further research to assess long-term outcomes and efficacy. Additionally, it suggests including implications for future research and clinical recommendations to enhance the depth and practical relevance of the review.
The Effect of Cerium Oxide (CeO2) on Ischemia-Reperfusion Injury in Skeletal Muscle in Mice with Streptozocin-Induced Diabetes
Authors: Özer Abdullah , Şengel Necmiye , Küçük Ayşegül , Yığman Zeynep , Özdemir Çağrı , Kılıç Yiğit , Dursun Ali Doğan , Bostancı Hasan , Kip Gülay , Arslan Mustafa
Published: May 2024

Objective: Lower extremity ischemia-reperfusion injury (IRI) may occur with trauma-related vascular injury and various vascular diseases, during the use of a tourniquet, in temporary clamping of the aorta in aortic surgery, or following acute or bilateral acute femoral artery occlusion. Mitochondrial dysfunction and increased basal oxidative stress in diabetes may cause an increase in the effects of increased reactive oxygen species (ROS) and mitochondrial dysfunction due to IRI. It is of great importance to examine therapeutic approaches that can minimize the effects of IRI, especially for patient groups under chronic oxidative stress such as DM. Cerium oxide (CeO2) nanoparticles mimic antioxidant enzymes and act as a catalyst that scavenges ROS. In this study, it was aimed to investigate whether CeO2 has protective effects on skeletal muscles in lower extremity IRI in mice with streptozocin-induced diabetes. Methods: A total of 38 Swiss albino mice were divided into six groups as follows: control group (group C, n = 6), diabetes group (group D, n = 8), diabetes–CeO2 (group DCO, n = 8), diabetes–ischemia/reperfusion (group DIR, n = 8), and diabetes–ischemia/reperfusion–CeO2 (group DIRCO, n = 8). The DCO and DIRCO groups were given doses of CeO2 of 0.5 mg/kg intraperitoneally 30 min before the IR procedure. A 120 min ischemia–120 min reperfusion period with 100% O2 was performed. At the end of the reperfusion period, muscle tissues were removed for histopathological and biochemical examinations. Results: Total antioxidant status (TAS) levels were found to be significantly lower in group DIR compared with group D (p = 0.047 and p = 0.022, respectively). In group DIRCO, total oxidant status (TOS) levels were found to be significantly higher than in group DIR (p < 0.001). The oxidative stress index (OSI) was found to be significantly lower in group DIR compared with group DCO (p < 0.001). Paraoxanase (PON) enzyme activity was found to be significantly increased in group DIR compared with group DCO (p < 0.001). The disorganization and degeneration score for muscle cells, inflammatory cell infiltration score, and total injury score in group DIRCO were found to be significantly lower than in group DIR (p = 0.002, p = 0.034, and p = 0.001, respectively). Conclusions: Our results confirm that CeO2, with its antioxidative properties, reduces skeletal muscle damage in lower extremity IRI in diabetic mice.
Analysis of Macular Vascularization Using Optical Coherence Tomography Angiography in Patients with Obstructive Sleep Apnea Syndrome: A Prospective Clinical Study
Authors: Abdessater Laura , Hein Matthieu , Rasquin Florence
Published: May 2024

Background and Objectives: Given the conflicting data available in the literature, this study aimed to investigate the impact of obstructive sleep apnea syndrome (OSAS) on the macular vascular density (VD) and perfusion density (PD). Materials and Methods: Based on the obstructive apnea–hypopnea index (OAHI), 61 prospectively recruited patients were assigned to either a control group (n = 12; OAHI < 5/h) or an OSAS group (n = 49; OAHI ≥ 5/h). The macular VD and PD of the superficial and deep capillary plexuses (SCP and DCP, respectively) were measured in the parafoveolar and perifoveolar areas using Zeiss PLEX Elite 9000 (6 × 6 mm). The values were compared between the control and OSAS groups. Results: Compared with the control group, the OSAS group demonstrated an increased VD of the DCP in the parafoveolar and perifoveolar areas and PD of the DCP in the perifoveolar area. No significant differences in either the macular VD or PD of the SCP were observed. There was no correlation between the OAHI and macular VD or PD. Conclusions: This study indicates that collateral vessel formation and possible retinal vasodilation occur in the DCP of patients with OSAS.
The Role of Oxidative Stress and Inflammatory Parameters in Heart Failure
Authors: Wróbel-Nowicka Karolina , Wojciechowska Celina , Jacheć Wojciech , Zalewska Marzena , Romuk Ewa
Published: May 2024

Heart failure (HF) remains a major medical and social problem. The NT-pro-brain natriuretic peptide (NT-proBNP) and its active form, brain-type natriuretic peptide (BNP), in a simple blood test are the gold-standard biomarkers for HF diagnosis. However, even good biomarkers such as natriuretic peptides fail to predict all the risks associated with HF due to the diversity of the mechanisms involved. The pathophysiology of HF is determined by numerous factors, including oxidative stress, inflammation, neuroendocrine activation, pathological angiogenesis, changes in apoptotic pathways, fibrosis and vascular remodeling. High readmission and mortality rates prompt a search for new markers for the diagnosis, prognosis and treatment of HF. Oxidative-stress-mediated inflammation plays a crucial role in the development of subsequent changes in the failing heart and provides a new insight into this complex mechanism. Oxidative stress and inflammatory biomarkers appear to be a promising diagnostic and prognostic tool in patients with HF. This systematic review provides an overview of the current knowledge about oxidative stress and inflammation parameters as markers of HF.
Enrichment of Nutmeg Essential Oil from Oil-in-Water Emulsions with PAN-Based Membranes
Authors: Yin Huilan , Zhang Haoyu , Cui Jiaoyang , Wu Qianlian , Huang Linlin , Qiu Jiaoyue , Zhang Xin , Xiang Yanyu , Li Bo , Liu Hongbo , Tang Zhishu , Zhang Yue , Zhu Huaxu
Published: May 2024

This study used polyacrylonitrile (PAN) and heat-treated polyacrylonitrile (H-PAN) membranes to enrich nutmeg essential oils, which have more complex compositions compared with common oils. The oil rejection rate of the H-PAN membrane was higher than that of the PAN membrane for different oil concentrations of nutmeg essential oil-in-water emulsions. After heat treatment, the H-PAN membrane showed a smaller pore size, narrower pore size distribution, a rougher surface, higher hydrophilicity, and higher oleophobicity. According to the GC-MS results, the similarities of the essential oils enriched by the PAN and H-PAN membranes to those obtained by steam distillation (SD) were 0.988 and 0.990, respectively. In addition, these two membranes also exhibited higher essential oil rejection for Bupleuri Radix, Magnolia Officinalis Cortex, Caryophylli Flos, and Cinnamomi Cortex essential oil-in-water emulsions. This work could provide a reference for membrane technology for the non-destructive separation of oil with complex components from oil-in-water emulsions.
Effect of Force and Heat Coupling on Machined Surface Integrity and Fatigue Performance of Superalloy GH4169 Specimens
Authors: Li Xun , Gou Ruijie , Zhang Ning
Published: May 2024

GH4169 is one of the key materials used to manufacture high-temperature load-bearing parts for aero-engines, and the surface integrity of these parts in service conditions significantly affects their high-temperature fatigue performance. Under a coupling effect of high temperature and alternating load, the evolution process of the machined surface integrity index of superalloy GH4169 specimens was studied, and fatigue performance tests at 20 °C, 450 °C, and 650 °C were carried out to analyze the primary factors affecting the high-temperature fatigue performance of specimens. The results indicated that the surface roughness of specimens remained essentially unchanged. However, the value of surface residual stress decreased significantly, with a release of more than 60% at the highest temperature. At 650 °C, the surface microhardness increased, while the degree of surface plastic deformation decreased under alternating loads. Simultaneously, when the surface roughness was less than Ra 0.4 μm, surface microhardness was the main factor affecting the high-temperature fatigue performance of specimens. The influence of surface microhardness on low-cycle fatigue performance was not consistent with that on high-cycle fatigue performance. The latter increased monotonically, whereas the former initially increased and then decreased with increasing surface microhardness.
Use of CAMS near Real-Time Aerosols in the HARMONIE-AROME NWP Model
Authors: Martín Pérez Daniel , Gleeson Emily , Maalampi Panu , Rontu Laura
Published: May 2024

Near real-time aerosol fields from the Copernicus Atmospheric Monitoring Services (CAMS), operated by the European Centre for Medium-Range Weather Forecasts (ECMWF), are configured for use in the HARMONIE-AROME Numerical Weather Prediction model. Aerosol mass mixing ratios from CAMS are introduced in the model through the first guess and lateral boundary conditions and are advected by the model dynamics. The cloud droplet number concentration is obtained from the aerosol fields and used by the microphysics and radiation schemes in the model. The results show an improvement in radiation, especially during desert dust events (differences of nearly 100 W/m2 are obtained). There is also a change in precipitation patterns, with an increase in precipitation, mainly during heavy precipitation events. A reduction in spurious fog is also found. In addition, the use of the CAMS near real-time aerosols results in an improvement in global shortwave radiation forecasts when the clouds are thick due to an improved estimation of the cloud droplet number concentration.
Silver Nanoparticles’ Localized Surface Plasmon Resonances Emerged in Polymeric Environments: Theory and Experiment
Authors: Tsarmpopoulou Maria , Ntemogiannis Dimitrios , Stamatelatos Alkeos , Geralis Dimitrios , Karoutsos Vagelis , Sigalas Mihail , Poulopoulos Panagiotis , Grammatikopoulos Spyridon
Published: May 2024

Considering that the plasmonic properties of metallic nanoparticles (NPs) are strongly influenced by their dielectric environment, comprehension and manipulation of this interplay are crucial for the design and optimization of functional plasmonic systems. In this study, the plasmonic behavior of silver nanoparticles encapsulated in diverse copolymer dielectric environments was investigated, focusing on the analysis of the emerging localized surface plasmon resonances (LSPRs) through both experimental and theoretical approaches. Specifically, two series of nanostructured silver ultrathin films were deposited via magnetron sputtering on heated Corning Glass substrates at 330 °C and 420 °C, respectively, resulting in the formation of self-assembled NPs of various sizes and distributions. Subsequently, three different polymeric layers were spin-coated on top of the silver NPs. Optical and structural characterization were carried out by means of UV–Vis spectroscopy and atomic force microscopy, respectively. Rigorous Coupled Wave Analysis (RCWA) was employed to study the LSPRs theoretically. The polymeric environment consistently induced a red shift as well as various alterations in the LSPR amplitude, suggesting the potential tunability of the system.
An Update on Zika Virus Vaccine Development and New Research Approaches
Authors: Buitrago-Pabón Angie Lizeth , Ruiz-Sáenz Salvador , Jiménez-Alberto Alicia , Aparicio-Ozores Gerardo , Castelán-Vega Juan Arturo , Ribas-Aparicio Rosa María
Published: May 2024

Zika virus (ZIKV) is an emerging flavivirus that represents significant public health challenges, particularly in the Americas, and is a substantial risk to other parts of the world due to its rapid expansion and its established association with neurological disorders, including Guillain–Barré syndrome and an intrauterine fetal infection that can cause microcephaly, blindness, and other congenital neurological complications. To date, no vaccine to prevent ZIKV infections has been approved. Therefore, developing a safe and effective vaccine against this virus is a global health priority. This review analyzes the ZIKV outbreaks, as well as associated neurological complications, its genome, and immunological responses. The current vaccines in development have reported results from preclinical and clinical trials about novel approaches to obtain safer and more effective vaccines and the challenges faced by ZIKV vaccine development.
Genetic Diversity of Plasmodium vivax Surface Ookinete Protein Pvs25 and Host Genes in Individuals Living along the Thai–Myanmar Border and Their Relationships with Parasite Density
Authors: Jalei Abdifatah Abdullahi , Chaijaroenkul Wanna , Na-Bangchang Kesara
Published: May 2024

Plasmodium vivax (Pv) accounts for over 50% of malaria cases in Latin America and Asia. Despite a significant reduction in Pv transmission in Thailand, the parasite remains endemic to the border areas. This study aimed to investigate the genetic diversity of the parasites and the host factors, as well as their relation to parasite density in Pvisolates, along the Thai–Myanmar border. Genetic variations in Pv markers, specifically the ookinete surface protein Pvs25, and host genes, including Toll-like receptor 6 (TLR6), TLR9, TIR Domain-containing adaptor protein (TIRAP), Toll-interacting protein (TOLLIP), Duffy antigen receptor for chemokines (DARC), and intercellular adhesion molecule 1 (ICAM-1), were investigated using polymerase chain reaction (PCR) with restriction fragment length polymorphism (RFLP). A total of 548 PCR-positive Pv samples collected from Tak and Kanchanaburi provinces during two periods (2006–2007 and 2014–2016) were included in the study. Pvs25 exhibited four haplotypes, with H1 (EGTKV) being the most prevalent in both provinces. Kanchanaburi isolates exhibited greater genetic diversity than Tak isolates. No significant deviations from neutrality were observed for Pvs25 in either area. ICAM-1 and TOLLIP s3750920 heterozygous carriers had greater median parasite densities than homozygous mutants. The TLR9 rs187084 T genotype had a significantly higher parasite density than the non-T genotype. The findings underscore the significant association between the rs3750920 C/T, rs5498 A/G, and rs187084 T genotypes and high parasite density in patients infected with Pv, highlighting their potentially critical role in malaria susceptibility.
Ovine and Caprine Strains of Corynebacterium pseudotuberculosis on Czech Farms—A Comparative Study
Authors: Markova Jirina , Langova Denisa , Babak Vladimir , Kostovova Iveta
Published: May 2024

Caseous lymphadenitis (CLA) is a worldwide disease of small ruminants caused by Corynebacterium pseudotuberculosis, a facultative intracellular pathogen that is able to survive and multiply in certain white blood cells of the host. In this study, 33 strains of C. pseudotuberculosis were isolated from sheep and goats suffering from CLA on nine farms in the Czech Republic. All these strains were tested for their antibiotic susceptibility, ability to form a biofilm and resistance to the effects of commonly used disinfectant agents. To better understand the virulence of C. pseudotuberculosis, the genomes of strains were sequenced and comparative genomic analysis was performed with another 123 genomes of the same species, including ovis and equi biovars, downloaded from the NCBI. The genetic determinants for the virulence factors responsible for adherence and virulence factors specialized for iron uptake and exotoxin phospholipase D were revealed in every analyzed genome. Carbohydrate-Active Enzymes were compared, revealing the presence of genetic determinants encoding exo-α-sialidase (GH33) and the CP40 protein in most of the analyzed genomes. Thirty-three Czech strains of C. pseudotuberculosis were identified as the biovar ovis on the basis of comparative genome analysis. All the compared genomes of the biovar ovis strains were highly similar regardless of their country of origin or host, reflecting their clonal behavior.
Genetic Analyses of Rare ESBL ST628 Klebsiella pneumoniae Detected during a Protracted Nosocomial Outbreak in the United Kingdom
Authors: Fordham Stephen Mark Edward , Drobniewski Francis , Barrow Magdalena , Hutchings Melissa , Crowther Kate , Richards Denise , Bolton Paul , Mantzouratou Anna , Sheridan Elizabeth
Published: May 2024

Klebsiella pneumoniae (K. pneumoniae) cultures from a hospital-wide outbreak in the UK, which lasted for over 12 months, were sequenced. We sought to sequence and genetically characterise the outbreak strain. Antibiotic Susceptibility Testing (AST) was performed on 65 K. pneumoniae isolates saved from the outbreak. All isolates were sequenced using the Oxford Nanopore Technologies (ONT) MinION flowcell: 10 isolates, including the isolate with the earliest collection date in 2017, were additionally sequenced on the NovaSeq 6000 platform to build high-accuracy nanopore-illumina assemblies. Among the sequenced strains, 60 were typed as ST628. 96.6% (n = 58/60) ST628 strains harboured a large ~247-kb FIB(K) plasmid carrying up to 11 antimicrobial resistance genes, including the extended-spectrum beta-lactamase (ESBL) gene, blaCTX-M-15. Clonality between the outbreak isolates was confirmed using single nucleotide polymorphism (SNP) typing. The outbreak strains were phylogenetically related to clinical ST628 strains identified in 2012, 6 years prior to the outbreak. A rare ESBL K. pneumoniae K2 ST628 strain harbouring a multi-drug resistant (MDR) plasmid encoding the ESBL gene blaCTX-M-15 was detected across multiple independent wards during the protracted nosocomial outbreak. Surveillance of this strain is recommended to prevent future nosocomial outbreaks.
Paulownia Witches’ Broom Disease: A Comprehensive Review
Authors: Zhang Yajie , Qiao Zesen , Li Jidong , Bertaccini Assunta
Published: May 2024

Phytoplasmas are insect-transmitted bacterial pathogens associated with diseases in a wide range of host plants, resulting in significant economic and ecological losses. Perennial deciduous trees in the genus Paulownia are widely planted for wood harvesting and ornamental purposes. Paulownia witches’ broom (PaWB) disease, associated with a 16SrI-D subgroup phytoplasma, is a destructive disease of paulownia in East Asia. The PaWB phytoplasmas are mainly transmitted by insect vectors in the Pentatomidae (stink bugs), Miridae (mirid bugs) and Cicadellidae (leafhoppers) families. Diseased trees show typical symptoms, such as branch and shoot proliferation, which together are referred to as witches’ broom. The phytoplasma presence affects the physiological and anatomical structures of paulownia. Gene expression in paulownia responding to phytoplasma presence have been studied at the transcriptional, post-transcriptional, translational and post-translational levels by high throughput sequencing techniques. A PaWB pathogenic mechanism frame diagram on molecular level is summarized. Studies on the interactions among the phytoplasma, the insect vectors and the plant host, including the mechanisms underlying how paulownia effectors modify processes of gene expression, will lead to a deeper understanding of the pathogenic mechanisms and to the development of efficient control measures.
An In-Depth Study on the Inhibition of Quorum Sensing by Bacillus velezensis D-18: Its Significant Impact on Vibrio Biofilm Formation in Aquaculture
Authors: Monzón-Atienza Luis , Bravo Jimena , Torrecillas Silvia , Gómez-Mercader Antonio , Montero Daniel , Ramos-Vivas José , Galindo-Villegas Jorge , Acosta Félix
Published: May 2024

Amid growing concerns about antibiotic resistance, innovative strategies are imperative in addressing bacterial infections in aquaculture. Quorum quenching (QQ), the enzymatic inhibition of quorum sensing (QS), has emerged as a promising solution. This study delves into the QQ capabilities of the probiotic strain Bacillus velezensis D-18 and its products, particularly in Vibrio anguillarum 507 communication and biofilm formation. Chromobacterium violaceum MK was used as a biomarker in this study, and the results confirmed that B. velezensis D-18 effectively inhibits QS. Further exploration into the QQ mechanism revealed the presence of lactonase activity by B. velezensis D-18 that degraded both long- and short-chain acyl homoserine lactones (AHLs). PCR analysis demonstrated the presence of a homologous lactonase-producing gene, ytnP, in the genome of B. velezensis D-18. The study evaluated the impact of B. velezensis D-18 on V. anguillarum 507 growth and biofilm formation. The probiotic not only controls the biofilm formation of V. anguillarum but also significantly restrains pathogen growth. Therefore, B. velezensis D-18 demonstrates substantial potential for preventing V. anguillarum diseases in aquaculture through its QQ capacity. The ability to disrupt bacterial communication and control biofilm formation positions B. velezensis D-18 as a promising eco-friendly alternative to conventional antibiotics in managing bacterial diseases in aquaculture.
Partial Characterization of Three Bacteriophages Isolated from Aquaculture Hatchery Water and Their Potential in the Biocontrol of Vibrio spp.
Authors: Yaşa İhsan , Evran Serap , Eren Eroğlu Asiye Esra , Önder Cengiz , Allahyari Maryam , Menderes Gülçin , Kullay Müberra
Published: May 2024

Bacteriophages are currently considered one of the most promising alternatives to antibiotics under the ‘One Health’ approach due to their ability to effectively combat bacterial infections. This study aimed to characterize Vibrio species in hatchery water samples collected from an aquaculture farm and investigate the biocontrol potential of their bacteriophages. Vibrio spp. (n = 32) isolates confirmed by LNA probe-based qPCR were used as hosts. Three Vibrio phages were isolated. IKEM_vK exhibited a broad host range, infecting V. harveyi (n = 8), V. alginolyticus (n = 2), V. azureus (n = 1), and V. ordalii (n = 1). IKEM_v5 showed lytic activity against V. anguillarum (n = 4) and V. ordalii (n = 1), while IKEM_v14 was specific to V. scophtalmi (n = 4). The morphological appearance of phages and their lytic effects on the host were visualized using scanning electron microscopy (SEM). All three phages remained relatively stable within the pH range of 6–11 and up to 60 °C. The lytic activities and biofilm inhibition capabilities of these phages against planktonic Vibrio cells support their potential applications in controlling vibriosis in aquaculture systems.
Molecular Detection of Cryptosporidium spp. and Microsporidia in Human and Animal Stool Samples
Authors: Gómez-Romano María Teresa , Rodríguez-Iglesias Manuel Antonio , Galán-Sánchez Fátima
Published: May 2024

Cryptosporidium spp. and Microsporidia are opportunistic microorganisms with remarkable zoonotic transmission potential due to their capacity to infect humans and animals. The aim of this study was to evaluate the prevalence of these microorganisms in stool samples of animal and human origin. In total, 369 stool samples (205 from human patients with diarrhea and 164 of animal origin) were included in the study. Cryptosporidium spp. and Microsporidia presence were determined by using multiplex nested PCR. Positive results were analyzed by using Sanger sequencing of the amplicon, utilizing BLASTN and ClustalX software to confirm identification. Cryptosporidium spp. were found in 0.97% and 4.26% of human and animal samples, respectively. Enterocytozoon bieneusi was detected in human and animal stools in 6.82% and 3.05% of the samples, respectively. No associations were found when analyzing the presence of Cryptosporidium spp. and E. bieneusi and the demographic and clinical variables of patients and animals. This study demonstrates the presence of these microorganisms in human and animal samples from different species, and the most interesting findings are the detection of Cryptosporidium spp. in pets (e.g., rodents) that are not usually included in this type of study, and the identification of E. bieneusi in patients with diarrhea without underlying disease.
High Detection Frequency of Vaccine-Associated Polioviruses and Non-Polio Enteroviruses in the Stools of Asymptomatic Infants from the Free State Province, South Africa
Authors: Mogotsi Milton T. , Ogunbayo Ayodeji E. , O’Neill Hester G. , Nyaga Martin M.
Published: May 2024

Enterovirus (EV) infections are widespread and associated with a range of clinical conditions, from encephalitis to meningitis, gastroenteritis, and acute flaccid paralysis. Knowledge about the circulation of EVs in neonatal age and early infancy is scarce, especially in Africa. This study aimed to unveil the frequency and diversity of EVs circulating in apparently healthy newborns from the Free State Province, South Africa (SA). For this purpose, longitudinally collected faecal specimens (May 2021–February 2022) from a cohort of 17 asymptomatic infants were analysed using metagenomic next-generation sequencing. Overall, seven different non-polio EV (NPEV) subtypes belonging to EV-B and EV-C species were identified, while viruses classified under EV-A and EV-D species could not be characterised at the sub-species level. Additionally, under EV-C species, two vaccine-related poliovirus subtypes (PV1 and PV3) were identified. The most prevalent NPEV species was EV-B (16/17, 94.1%), followed by EV-A (3/17, 17.6%), and EV-D (4/17, 23.5%). Within EV-B, the commonly identified NPEV types included echoviruses 6, 13, 15, and 19 (E6, E13, E15, and E19), and coxsackievirus B2 (CVB2), whereas enterovirus C99 (EV-C99) and coxsackievirus A19 (CVA19) were the only two NPEVs identified under EV-C species. Sabin PV1 and PV3 strains were predominantly detected during the first week of birth and 6–8 week time points, respectively, corresponding with the OPV vaccination schedule in South Africa. A total of 11 complete/near-complete genomes were identified from seven NPEV subtypes, and phylogenetic analysis of the three EV-C99 identified revealed that our strains were closely related to other strains from Cameroon and Brazil, suggesting global distribution of these strains. This study provides an insight into the frequency and diversity of EVs circulating in asymptomatic infants from the Free State Province, with the predominance of subtypes from EV-B and EV-C species. This data will be helpful to researchers looking into strategies for the control and treatment of EV infection.
Plasmid-Borne Biosynthetic Gene Clusters within a Permanently Stratified Marine Water Column
Authors: Mara Paraskevi , Geller-McGrath David , Suter Elizabeth , Taylor Gordon T. , Pachiadaki Maria G. , Edgcomb Virginia P.
Published: May 2024

Plasmids are mobile genetic elements known to carry secondary metabolic genes that affect the fitness and survival of microbes in the environment. Well-studied cases of plasmid-encoded secondary metabolic genes in marine habitats include toxin/antitoxin and antibiotic biosynthesis/resistance genes. Here, we examine metagenome-assembled genomes (MAGs) from the permanently-stratified water column of the Cariaco Basin for integrated plasmids that encode biosynthetic gene clusters of secondary metabolites (smBGCs). We identify 16 plasmid-borne smBGCs in MAGs associated primarily with Planctomycetota and Pseudomonadota that encode terpene-synthesizing genes, and genes for production of ribosomal and non-ribosomal peptides. These identified genes encode for secondary metabolites that are mainly antimicrobial agents, and hence, their uptake via plasmids may increase the competitive advantage of those host taxa that acquire them. The ecological and evolutionary significance of smBGCs carried by prokaryotes in oxygen-depleted water columns is yet to be fully elucidated.