bluuebunny/crossref_metadata_2025 huggingface dataset:
Adjustment of Photosynthetic and Antioxidant Activities to Water Deficit Is Crucial in the Drought Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms
Authors: Lechowicz Katarzyna , Pawłowicz Izabela , Perlikowski Dawid , Arasimowicz-Jelonek Magdalena , Blicharz Sara , Skirycz Aleksandra , Augustyniak Adam , Malinowski Robert , Rapacz Marcin , Kosmala Arkadiusz
Published: August 2020

Lolium multiflorum/Festuca arundinacea introgression forms have been proved several times to be good models to identify key components of grass metabolism involved in the mechanisms of tolerance to water deficit. Here, for the first time, a relationship between photosynthetic and antioxidant capacities with respect to drought tolerance of these forms was analyzed in detail. Two closely related L. multiflorum/F. arundinacea introgression forms distinct in their ability to re-grow after cessation of prolonged water deficit in the field were selected and subjected to short-term drought in pots to dissect precisely mechanisms of drought tolerance in this group of plants. The studies revealed that the form with higher drought tolerance was characterized by earlier and higher accumulation of abscisic acid, more stable cellular membranes, and more balanced reactive oxygen species metabolism associated with a higher capacity of the antioxidant system under drought conditions. On the other hand, both introgression forms revealed the same levels of stomatal conductance, CO2 assimilation, and consequently, intrinsic water use efficiency under drought and recovery conditions. However, simultaneous higher adjustment of the Calvin cycle to water deficit and reduced CO2 availability, with respect to the accumulation and activity of plastid fructose-1,6-bisphosphate aldolase, were clearly visible in the form with higher drought tolerance.
The Importance of Telomere Shortening for Atherosclerosis and Mortality
Authors: Herrmann Wolfgang , Herrmann Markus
Published: August 2020

Telomeres are the protective end caps of chromosomes and shorten with every cell division. Short telomeres are associated with older age and adverse lifestyle factors. Leucocyte telomere length (LTL) has been proposed as a biomarker of biological age. The shortening of LTL with age is the result of the end-replication problem, environmental, and lifestyle-related factors. Epidemiologic studies have shown that LTL predicts cardiovascular disease, all-cause mortality, and death from vascular causes. Age appears to be an important co-variate that explains a substantial fraction of this effect. Although it has been proposed that short telomeres promote atherosclerosis and impair the repair of vascular lesions, existing results are inconsistent. Oxidative stress and chronic inflammation can both accelerate telomere shortening. Multiple factors, including homocysteine (HCY), vitamin B6, and vitamin B12 modulate oxidative stress and inflammation through direct and indirect mechanisms. This review provides a compact overview of telomere physiology and the utility of LTL measurements in atherosclerosis and cardiovascular disease. In addition, it summarizes existing knowledge regarding the impact of oxidative stress, inflammation, HCY, and B-vitamins on telomere function.
Diagnostic and Prognostic Value of Conventional Brain MRI in the Clinical Work-Up of Patients with Amyotrophic Lateral Sclerosis
Authors: Rizzo Giovanni , Marliani Anna , Battaglia Stella , Albini Riccioli Luca , De Pasqua Silvia , Vacchiano Veria , Infante Rossella , Avoni Patrizia , Donadio Vincenzo , Passaretti Massimiliano , Bartolomei Ilaria , Salvi Fabrizio , Liguori Rocco ,   on behalf of the BoReALS group
Published: August 2020

Clinical signs of upper motor neuron (UMN) involvement are important in the diagnosis of amyotrophic lateral sclerosis (ALS) though are often difficult to analyze. Many studies using both qualitative and quantitative evaluations have reported abnormal Magnetic Resonance Imaging (MRI) findings at the level of the pyramidal pathway in patients with ALS. Although the most interesting results were obtained by quantitative studies using advanced MR techniques, the qualitative evaluation of MRI images remains the most-used in clinical practice. We evaluated the diagnostic and prognostic contribution of conventional 3T-MRI in the clinical work-up of ALS patients. Two neuroradiologists retrospectively assessed 3T-MRI data of 93 ALS patients and 89 controls. The features of interest were corticospinal tract (CST) T2/FLAIR hyperintensity, motor cortex (MC) T2*/SWI hypointensity, and selective MC atrophy. All MRI features were significantly more prevalent in ALS patients than in controls. The simultaneous presence of CST FLAIR hyperintensity and MC SWI hypointensity was associated with the highest diagnostic accuracy (sensitivity: 70%; specificity: 81%; positive predictive value, PPV: 90%; negative predictive value, NPV: 51%; accuracy: 73%) and a shorter survival (HR: 6.56, p = 0.002). Conventional 3T-MRI can be a feasible tool to detect specific qualitative changes based on UMN involvement and to support clinical diagnosis of ALS. Importantly, CST FLAIR hyperintensity and MC SWI hypointensity are predictors of shorter survival in ALS patients.
Effects of Hoverboard on Balance in Young Soccer Athletes
Authors: Moffa Stefano , Perna Angelica , Candela Gabriele , Cattolico Alessandro , Sellitto Carmine , De Blasiis Paolo , Guerra Germano , Tafuri Domenico , Lucariello Angela
Published: August 2020

Hoverboards are always more popular among children. Hoverboards are to them like a game or a mean of transport, but they could be used as a valid and useful instrument in children’s training programs to improve their performance. In this study, we compared the athletic performance of two groups of 12 children. A total of 24 children aged between 8 and 11 years followed a similar training program for five months, but the first group used a hoverboard (Hb+ group: Age: Standard Deviation (SD) = 1.15 Mean = 9.66; Weight: SD = 5.90 Mean = 32; Height: SD = 7.64 Mean = 135.08) for some of the training time, differently from the second group (Hb- group: Age: SD = 1.15 Mean = 9.66; Weight: SD = 5.82 Mean = 31.16; Height: SD = 7.66 Mean = 136.16), which never used it. All of the children were asked to complete three tests (one leg test, stork test and balance beam walking test) before starting their own training program and after five months, to evaluate how their performances changed in terms of time. Comparing the recorded time difference between T0 and T1 of the Hb+ group with the same difference measured in Hb- group, it was found that there was a statistically significant difference (p value < 0.05) between these data for all three tests. Children who used the hoverboard in their training program achieved better result than children who did not use it. In the future, the hoverboard could help athletes to improve their performances, possibly applying it not only in football training, but even in other sports.
Lime-Based Mortar Reinforced by Randomly Oriented Short Fibers for the Retrofitting of the Historical Masonry Structure
Authors: Angiolilli Michele , Gregori Amedeo , Vailati Marco
Published: August 2020

Recent seismic events prompted research to develop innovative materials for strengthening and repair of both modern and historic masonry constructions (buildings, bridges, towers) and structural components (walls, arches and vaults, pillars, and columns). Strengthening solutions based on composite materials, such as the Fiber Reinforced Polymers (FRP) or the Fiber Reinforced Cementitious Matrix (FRCM), have been increasingly considered in the last two decades. Despite reinforcement made of short-fibers being a topic that has been studied for several years from different researchers, it is not yet fully considered for the restoration of the masonry construction. This work aims to experimentally investigate the enhancement of the mechanical properties of lime-based mortar reinforced by introducing short glass fibers in the mortar matrix with several contents and aspect ratios. Beams with dimensions of 160 mm × 40 mm × 40 mm with a central notch were tested in three-point bending configuration aiming to evaluate both the flexural strength and energy fracture of the composite material. Then, the end pieces of the broken beams were tested in Brazilian and compressive tests. All the tests were performed by a hydraulic displacement-controlled testing machine. Results highlight that the new composite material ensures excellent ductility capacity and it can be considered a promising alternative to the classic fiber-reinforcing systems.
Microstructure and Strengthening Model of Cu–Fe In-Situ Composites
Authors: Liu Keming , Sheng Xiaochun , Li Qingpeng , Zhang Mengcheng , Han Ningle , He Guangyu , Zou Jin , Chen Wei , Atrens Andrej
Published: August 2020

The tensile strength evolution and strengthening mechanism of Cu–Fe in-situ composites were investigated using both experiments and theoretical analysis. Experimentally, the tensile strength evolution of the in-situ composites with a cold deformation strain was studied using the model alloys Cu–11Fe, Cu–14Fe, and Cu–17Fe, and the effect of the strain on the matrix of the in-situ composites was studied using the model alloys Cu–3Fe and Cu–4.3Fe. The tensile strength was related to the microstructure and to the theoretical strengthening mechanisms. Based on these experimental data and theoretical insights, a mathematical model was established for the dependence of the tensile strength on the cold deformation strain. For low cold deformation strains, the strengthening mechanism was mainly work hardening, solid solution, and precipitation strengthening. Tensile strength can be estimated using an improved rule of mixtures. For high cold deformation strains, the strengthening mechanism was mainly filament strengthening. Tensile strength can be estimated using an improved Hall–Petch relation.
Influence of Different Lithium Compounds on Hydration and Mechanical Properties of Calcium Sulfoaluminate Cement
Authors: Deng Hongyang , Wei Xuanchun , Liu Shaoyan , Li Shan , Cai Xinhua
Published: August 2020

This work investigated the influence of three different lithium compounds, lithium carbonate (Li2CO3), lithium sulfate (Li2SO4) and lithium chloride (LiCl), on the hydration and mechanical properties of calcium sulfoaluminate (CSA) cement mixtures. Five concentrations of Li+, 0, 0.05, 0.11, 0.16 and 0.22 mmol/g of cement, were chosen, and then the proportions (by mass) of three lithium compounds were determined. Compressive strengths at 8 h, 24 h and 28 days were tested. Meanwhile, an early hydration heat test, thermogravimetric (TG) analysis, X-ray diffraction (XRD) and scanning electron microscope (SEM) techniques were performed to study the influences of different lithium compounds on properties of CSA cement mixtures. The experimental results show that three lithium compounds can all accelerate the early hydration process of CSA cement. There is not a remarkable difference on the properties of CSA cement pastes with a different content of Li+. The anion of lithium compounds can also affect the properties of CSA cement pastes, the accelerating effects of LiCl and Li2SO4 are more significant than that of Li2CO3, but there is not a distinct difference between LiCl and Li2SO4.
Liquid Platelet-Rich Fibrin and Heat-Coagulated Albumin Gel: Bioassays for TGF-β Activity
Authors: Kargarpour Zahra , Nasirzade Jila , Panahipour Layla , Miron Richard J. , Gruber Reinhard
Published: August 2020

Liquid platelet-rich fibrin (PRF) can be prepared by high centrifugation forces separating the blood into a platelet-poor plasma (PPP) layer and a cell-rich buffy coat layer, termed concentrated PRF (C-PRF). Heating the liquid PPP was recently introduced to prepare an albumin gel (Alb-gel) that is later mixed back with the concentrated liquid C-PRF to generate Alb-PRF. PRF is a rich source of TGF-β activity; however, the overall TGF-β activity in the PPP and the impact of heating the upper plasma layer remains unknown. Here, we investigated for the first time the in vitro TGF-β activity of all fractions of Alb-PRF. We report that exposure of oral fibroblasts with lysates of PPP and the buffy coat layer, but not with heated PPP, provoked a robust increase in the TGF-β target genes interleukin 11 and NADPH oxidase 4 by RT-PCR, and for IL11 by immunoassay. Consistent with the activation of TGF-β signaling, expression changes were blocked in the presence of the TGF-β receptor type I kinase inhibitor SB431542. Immunofluorescence and Western blot further confirmed that lysates of PPP and the buffy coat layer, but not heated PPP, induced the nuclear translocation of Smad2/3 and increased phosphorylation of Smad3. The immunoassay further revealed that PPP and particularly BC are rich in active TGF-β compared to heated PPP. These results strengthen the evidence that not only the cell-rich C-PRF but also PPP comprise a TGF-β activity that is, however, heat sensitive. It thus seems relevant to mix the heated PPP with the buffy coat C-PRF layer to regain TGF-β activity, as proposed during the preparation of Alb-PRF.
A Review of the Mechanical Properties and Durability of Ecological Concretes in a Cold Climate in Comparison to Standard Ordinary Portland Cement-Based Concrete
Authors: Kothari Ankit , Habermehl-Cwirzen Karin , Hedlund Hans , Cwirzen Andrzej
Published: August 2020

Most of the currently used concretes are based on ordinary Portland cement (OPC) which results in a high carbon dioxide footprint and thus has a negative environmental impact. Replacing OPCs, partially or fully by ecological binders, i.e., supplementary cementitious materials (SCMs) or alternative binders, aims to decrease the carbon dioxide footprint. Both solutions introduced a number of technological problems, including their performance, when exposed to low, subfreezing temperatures during casting operations and the hardening stage. This review indicates that the present knowledge enables the production of OPC-based concretes at temperatures as low as −10 °C, without the need of any additional measures such as, e.g., heating. Conversely, composite cements containing SCMs or alkali-activated binders (AACs) showed mixed performances, ranging from inferior to superior in comparison with OPC. Most concretes based on composite cements require pre/post heat curing or only a short exposure to sub-zero temperatures. At the same time, certain alkali-activated systems performed very well even at −20 °C without the need for additional curing. Chemical admixtures developed for OPC do not always perform well in other binder systems. This review showed that there is only a limited knowledge on how chemical admixtures work in ecological concretes at low temperatures and how to accelerate the hydration rate of composite cements containing high amounts of SCMs or AACs, when these are cured at subfreezing temperatures.
Comparative Study on the Influence of Noble Metal Nanoparticles (Ag, Au, Pd) on the Photocatalytic Activity of ZnO NPs Embedded in Renewable Castor Oil Polymer Matrices
Authors: Chibac-Scutaru Andreea L. , Podasca Viorica , Timpu Daniel , Melinte Violeta
Published: August 2020

Hybrid polymeric materials, due to the unique combination of properties that can be obtained by the convenient variation of organic and inorganic components, represent an attractive alternative for many applications, especially photocatalysis. Herein, we report the preparation of nanocomposite films containing functionalized ZnO nanoparticles, as well as in situ photogenerated noble metal nanoparticles (Ag, Au, Pd), for the achieving of materials with enhanced photocatalytic activity under visible light. The flexible free-standing nanocomposite films were synthesized by photopolymerization of a monomer mixture (silane castor oil urethane dimethacrylate and polypropylene oxide urethane dimethacrylate) in the presence of a Irgacure 819 photoinitiator. The efficiency of ZnO NPs functionalization was established by Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis, while the polymer composites were characterized by UV-Vis spectroscopy, X-ray diffraction, transmission electron microscopy and scanning electron microscopy to evidence the formation, size and distribution of the nanoparticles inside the photocrosslinked matrix. To establish the photocatalytic capacity of nanocomposite films, the decomposition of various pollutants (methyl orange, phenol, metronidazole) was monitored under visible light irradiation, the best results being obtained for Au/ZnO film. Also, the advantage of immobilizing the catalysts in a polymeric support and its recycling ability without a significant decrease in photocatalytic efficiency was analysed.
Aluminum-to-Steel Cladding by Explosive Welding
Authors: H. S. F. L. Carvalho Gustavo , Galvão Ivan , Mendes Ricardo , M. Leal Rui , Loureiro Altino
Published: August 2020

The production of aluminum-carbon steel and aluminum-stainless steel clads is challenging, and explosive welding is one of the most suitable processes to achieve them. The present work aims to investigate the coupled effect of two strategies for optimizing the production of these clads by explosive welding: the use of a low-density interlayer and the use of a low-density and low-detonation velocity explosive mixture. A broad range of techniques was used to characterize the microstructural and the mechanical properties of the welds, specifically, optical microscopy, scanning electron microscopy, energy dispersive spectroscopy, electron backscatter diffraction, microhardness and tensile-shear testing with digital image correlation analysis. Although aluminum-carbon steel and aluminum-stainless steel have different weldabilities, clads with sound microstructure and good mechanical behavior were achieved for both combinations. These results were associated with the low values of collision point and impact velocities provided by the tested explosive mixture, which made the weldability difference between these combinations less significant. The successful testing of this explosive mixture indicates that it is suitable to be used for welding very thin flyers and/or dissimilar materials that easily form intermetallic phases.
Metabolic Profiles of Whole, Parotid and Submandibular/Sublingual Saliva
Authors: Meleti Marco , Quartieri Eleonora , Antonelli Rita , Pezzi Margherita E. , Ghezzi Benedetta , Viani Maria Vittoria , Setti Giacomo , Casali Emanuela , Ferrari Elena , Ciociola Tecla , Spisni Alberto , Pertinhez Thelma A.
Published: August 2020

The detection of salivary molecules associated with pathological and physiological alterations has encouraged the search of novel and non-invasive diagnostic biomarkers for oral health evaluation. While genomic, transcriptomic, and proteomic profiles of human saliva have been reported, its metabolic composition is a topic of research: metabolites in submandibular/sublingual saliva have never been analyzed systematically. In this study, samples of whole, parotid, and submandibular/sublingual saliva from 20 healthy donors, without dental or periodontal diseases, were examined by nuclear magnetic resonance. We identified metabolites which are differently distributed within the three saliva subtypes (54 in whole, 49 in parotid, and 36 in submandibular/sublingual saliva). Principal component analysis revealed a distinct cluster for whole saliva and a partial overlap for parotid and submandibular/sublingual metabolites. We found exclusive metabolites for each subtype: 2-hydroxy-3-methylvalerate, 3-methyl-glutarate, 3-phenylpropionate, 4-hydroxyphenylacetate, 4-hydroxyphenyllactate, galactose, and isocaproate in whole saliva; caprylate and glycolate in submandibular/sublingual saliva; arginine in parotid saliva. Salivary metabolites were classified into standard and non-proteinogenic amino acids and amines; simple carbohydrates; organic acids; bacterial-derived metabolites. The identification of a salivary gland-specific metabolic composition in healthy people provides the basis to invigorate the search for salivary biomarkers associated with oral and systemic diseases.
The Mechanism of Layer Stacked Clamping (LSC) for Polishing Ultra-Thin Sapphire Wafer
Authors: Chen Zhixiang , Cao Linlin , Yuan Julong , Lyu Binghai , Hang Wei , Wang Jiahuan
Published: August 2020

Double-sides polishing technology has the advantages of high flatness and parallelism, and high polishing efficiency. It is the preferred polishing method for the preparation of ultra-thin sapphire wafer. However, the clamping method is a fundamental problem which is currently difficult to solve. In this paper, a layer stacked clamping (LSC) method of ultra-thin sapphire wafer which was used on double-sides processing was proposed and the clamping mechanism of layer stacked clamping (LSC) was studied. Based on the rough surface contact model of fractal theory, combining the theory of van der Waals force and capillary force, the adhesion model of the rough surfaces was constructed, and the reliability of the model was verified through experiments. Research has found that after displacement between the two surfaces the main force of the adhesion force is capillary force. The capillary force decreases with the increasing of surface roughness, droplet volume, and contact angle. For an ultra-thin sapphire wafer with a diameter of 50.8 mm and a thickness of 0.17 mm, more than 1.4 N of normal adhesion force can be generated through the LSC method. Through the double-sides polishing experiment using the LSC method, an ultra-thin sapphire wafer with an average surface roughness (Ra) of 1.52 nm and a flatness (PV) of 0.968 μm was obtained.
Rapid Room-Temperature Preparation of Hierarchically Porous Metal–Organic Frameworks for Efficient Uranium Removal from Aqueous Solutions
Authors: Duan Chongxiong , Zhang Yi , Li Jiexin , Kang Le , Xie Yawei , Qiao Wenxiong , Zhu Chunxia , Luo Haochuan
Published: August 2020

The effective removal of uranium from an aqueous solution is a highly valuable process for the environment and health. In this study, we developed a facile and rapid method to synthesize hierarchically porous Cu-BTC (RT-Cu-BTC) using a cooperative template strategy. The as-synthesized RT-Cu-BTC exhibited hierarchically porous structure and excellent thermostability, as revealed by X-ray powder diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis. Compared with conventional metal–organic frameworks (MOFs) and zeolites, the obtained RT-Cu-BTC exhibited enhanced adsorption capacity (839.7 mg·g−1) and high removal efficiency (99.8%) in the capture of uranium (VI) from aqueous solutions. Furthermore, the conditions such as adsorbent dose, contact time, and temperature in adsorption of uranium (VI) by RT-Cu-BTC were investigated in detail. The thermodynamics data demonstrated the spontaneous and endothermic nature of the uranium (VI) adsorption process. The Langmuir isotherm and pseudo-second-order models could better reflect the adsorption process of uranium (VI) onto RT-Cu-BTC. In addition, the as-synthesized RT-Cu-BTC showed excellent stability in removing uranium (VI) from an aqueous solution. This work provides a facile and rapid approach for fabricating hierarchically porous MOFs to realize a highly efficient removal of uranium (VI) from aqueous systems.
Nitrogen- and Oxygen-Containing Three-Dimensional Hierarchical Porous Graphitic Carbon for Advanced Supercapacitor
Authors: Zhao Yunyan , Wang Honghu , Liu Jing , Liu Jinghao , Li Guicun , Peng Hongrui , Chen Kezheng , Zhang Zhonghua
Published: August 2020

Three-dimensional hierarchical porous graphitic carbon (HPGC) were synthesized via one-step carbonization-activation and a catalytic strategy. The method can not only improve the graphitization degree of carbon materials, but also offer plentiful interfaces for charge accumulation and short paths for ion/electron transport. Polypyrrole, potassium hydroxide, and nickel acetate were used as the carbon precursors, activating agent, and catalyst, respectively. The retraction and dissolution of Ni caused the change of pore size in the material and led to the interconnected micro/nano holes. Nickel acetate played a significant role in enhancing the electrical conductivity, introducing pseudocapacitance, and promoting ion diffusion. In the supercapacitor, HPGC electrode exhibited a remarkable specific capacitance of 336.3 F g−1 under 0.5 A g−1 current density and showed high rate capability, even with large current densities applied (up to 50 A g−1). Moreover, HPGC showed optimal cycling stability with 97.4% capacitance retention followed by 3000 charge-discharge cycles. The excellent electrochemical performances coupled with a facile large-scale synthesis procedure make HPGC a promising alternative for supercapacitors.
Nuts and their Effect on Gut Microbiota, Gut Function and Symptoms in Adults: A Systematic Review and Meta-Analysis of Randomised Controlled Trials
Authors: Creedon Alice C. , Hung Estella S. , Berry Sarah E. , Whelan Kevin
Published: August 2020

Nuts contain fibre, unsaturated fatty acids and polyphenols that may impact the composition of the gut microbiota and overall gut health. This study aimed to assess the impact of nuts on gut microbiota, gut function and gut symptoms via a systematic review and meta-analysis of randomised controlled trials (RCTs) in healthy adults. Eligible RCTs were identified by systematic searches of five electronic databases, hand searching of conference abstracts, clinical trials databases, back-searching reference lists and contact with key stakeholders. Eligible studies were RCTs administering tree nuts or peanuts in comparison to control, measuring any outcome related to faecal microbiota, function or symptoms. Two reviewers independently screened papers, performed data extraction and risk of bias assessment. Outcome data were synthesised as weighted mean difference (WMD) or standardised mean difference (SMD) using a random effects model. This review was registered on PROSPERO (CRD42019138169). Eight studies reporting nine RCTs were included, investigating almonds (n = 5), walnuts (n = 3) and pistachios (n = 1). Nut consumption significantly increased Clostridium (SMD: 0.40; 95% CI, 0.10, 0.71; p = 0.01), Dialister (SMD: 0.44; 95% CI, 0.13, 0.75; p = 0.005), Lachnospira (SMD: 0.33; 95% CI, 0.02, 0.64; p = 0.03) and Roseburia (SMD: 0.36; 95% CI, 0.10, 0.62; p = 0.006), and significantly decreased Parabacteroides (SMD: −0.31; 95% CI, −0.62, −0.00; p = 0.05). There was no effect of nuts on bacterial phyla, diversity or stool output. Further parallel design RCTs, powered to detect changes in faecal microbiota and incorporating functional and clinical outcomes, are needed.
The ω-3 Polyunsaturated Fatty Acids and Oxidative Stress in Long-Term Parenteral Nutrition Dependent Adult Patients: Functional Lipidomics Approach
Authors: Kosek Vit , Heczkova Marie , Novak Frantisek , Meisnerova Eva , Novákova Olga , Zelenka Jaroslav , Bechynska Kamila , Vrzacova Nikola , Suttnar Jiri , Hlavackova Alzbeta , Dankova Helena , Bratova Miriam , Daskova Nikola , Malinska Hana , Oliyarnyk Olena , Wohl Petr , Bastova Hana , Hajslova Jana , Cahova Monika
Published: August 2020

Omega-3 polyunsaturated fatty acids (ω-3PUFAs) are introduced into parenteral nutrition (PN) as hepatoprotective but may be susceptible to the lipid peroxidation while olive oil (OO) is declared more peroxidation resistant. We aimed to estimate how the lipid composition of PN mixture affects plasma and erythrocyte lipidome and the propensity of oxidative stress. A cross-sectional comparative study was performed in a cohort of adult patients who were long-term parenterally administered ω-3 PUFAs without (FO/–, n = 9) or with (FO/OO, n = 13) olive oil and healthy age- and sex-matched controls, (n = 30). Lipoperoxidation assessed as plasma and erythrocyte malondialdehyde content was increased in both FO/– and FO/OO groups but protein oxidative stress (protein carbonyls in plasma) and low redox status (GSH/GSSG in erythrocytes) was detected only in the FO/– subcohort. The lipidome of all subjects receiving ω-3 PUFAs was enriched with lipid species containing ω-3 PUFAs (FO/–˃FO/OO). Common characteristic of all PN-dependent patients was high content of fatty acyl-esters of hydroxy-fatty acids (FAHFAs) in plasma while acylcarnitines and ceramides were enriched in erythrocytes. Plasma and erythrocyte concentrations of plasmanyls and plasmalogens (endogenous antioxidants) were decreased in both patient groups with a significantly more pronounced effect in FO/–. We confirmed the protective effect of OO in PN mixtures containing ω-3 PUFAs.
Layered Silicate-Alginate Composite Particles for the pH-Mediated Release of Theophylline
Authors: Nandi Uttom , Trivedi Vivek , Douroumis Dennis , Mendham Andrew P. , Coleman Nichola J.
Published: August 2020

Numerous natural and synthetic clay minerals have proven to be excellent drug carriers for high drug-loaded and sustained release formulations due to their considerable ion exchange, adsorption, and swelling capacities. Moreover, the synthetic smectite clays have added advantages in terms of compositional purity and controlled cation exchange capacity in comparison to natural clays. This study involves the intercalation of theophylline (TP) in a synthetic clay, Laponite® (LP), followed by the inclusion of the resulting intercalates into sodium alginate (SA) beads to achieve pH-controlled drug release. Maximum intercalated drug incorporation of 68 mg/g was obtained by ion exchange at pH 1.2 and confirmed by an increase in basal spacing of the clay from 12.9 to 15.5 Å. TP release from the binary LP-TP intercalates in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) was found to be 40% and 70%, respectively. LP-TP particles were also incorporated in an SA matrix via polymer crosslinking using CaCl2(aq) to improve the pH selective release. The ternary polymer-clay-drug composite particles effectively prevented the release of TP at low pH in SGF and resulted in sustained release in SIF, with 40% dissolution within 120 min.
Deciphering the Pharmacological Properties of Methanol Extract of Psychotria calocarpa Leaves by In Vivo, In Vitro and In Silico Approaches
Authors: Bristy Tahmina Akter , Barua Niloy , Montakim Tareq Abu , Sakib Shahenur Alam , Etu Saida Tasnim , Chowdhury Kamrul Hasan , Jyoti Mifta Ahmed , Aziz Md. Arfin Ibn , Reza A.S.M. Ali , Caiazzo Elisabetta , Romano Barbara , Tareq Syed Mohammed , Emran Talha Bin , Capasso Raffaele
Published: August 2020

The present study explores the neuropharmacological, antinociceptive, antidiarrheal, antioxidant, thrombolytic and cytotoxic activity of methanol extract of Psychotria calocarpa leaves (MEPC). In anxiolytic activity testing of MEPC by elevated plus maze test, hole–board test and light–dark test, the extract exhibited a dose-dependent reduction of anxiety while the open field test observed a decreased locomotion. The administration of MEPC revealed a significant dose-dependent reduction of depressant behavior in forced swimming and tail suspension test. Additionally, the antinociceptive and antidiarrheal activity exposed a significant reduction of nociception and diarrheal behavior at the highest dose. In addition, a strong antioxidant activity was observed in DPPH-free radical-scavenging assay (IC50 = 461.05 μg/mL), total phenol content (118.31 ± 1.12 mg) and total flavonoid content (100.85 ± 0.97 mg). The significant clot–lysis activity was also observed with moderate toxicity (LC50 = 247.92 μg/mL) level in the lethality assay of brine shrimp. Moreover, in silico molecular docking study showed that the compound Psychotriasine could offer promising active site interactions for binding proteins. Furthermore, ADME/T and toxicological properties of the compound satisfied the Lipinski’s rule of five and Veber rules for drug-like potential and toxicity level. Overall, MEPC had a potential neuropharmacological, antinociceptive, antidiarrheal and antioxidant activity that warranted further investigation.
Therapeutic Efficacy of Curcumin Enhanced by Microscale Discoidal Polymeric Particles in a Murine Asthma Model
Authors: Park Jun Young , Chu Ga Eul , Park Sanghyo , Park Chaewon , Aryal Susmita , Kang Won Jun , Cho Won Gil , Key Jaehong
Published: August 2020

Curcumin is considered a potential anti-asthmatic agent owing to its anti-inflammatory properties. The objective of the present study was to prepare curcumin-containing poly(lactic-co-glycolic acid)-based microscale discoidal polymeric particles (Cur-PLGA-DPPs) and evaluate their anti-asthmatic properties using a murine asthma model. Cur-PLGA-DPPs were prepared using a top-down fabrication method. The prepared Cur-PLGA-DPPs had a mean particle size of 2.5 ± 0.4 μm and a zeta potential value of −34.6 ± 4.8 mV. Ex vivo biodistribution results showed that the Cur-PLGA-DPPs mainly accumulated in the lungs and liver after intravenous injection. Treatment with Cur-PLGA-DPPs effectively suppressed the infiltration of inflammatory cells in bronchoalveolar lavage fluid, and reduced bronchial wall thickening and goblet-cell hyperplasia compared to those in the phosphate-buffered-saline-treated control group. No significant changes in hematology and blood biochemistry parameters were observed after treatment with Cur-PLGA-DPPs. At equal curcumin concentrations, treatment with Cur-PLGA-DPPs exhibited better therapeutic efficacy than treatment with free curcumin. Our results suggest that the microscale Cur-PLGA-DPPs can be potentially used as a lung-targeted asthma therapy.
Design and Characterization of Ethosomes for Transdermal Delivery of Caffeic Acid
Authors: Hallan Supandeep Singh , Sguizzato Maddalena , Mariani Paolo , Cortesi Rita , Huang Nicolas , Simelière Fanny , Marchetti Nicola , Drechsler Markus , Ruzgas Tautgirdas , Esposito Elisabetta
Published: August 2020

The present investigation describes a formulative study aimed at designing ethosomes for caffeic acid transdermal administration. Since caffeic acid is characterized by antioxidant potential but also high instability, its encapsulation appears to be an interesting strategy. Ethosomes were produced by adding water into a phosphatidylcholine ethanol solution under magnetic stirring. Size distribution and morphology of ethosome were investigated by photon correlation spectroscopy, small-angle X-ray spectroscopy, and cryogenic transmission electron microscopy, while the entrapment capacity of caffeic acid was evaluated by high-performance liquid chromatography. Caffeic acid stability in ethosome was compared to the stability of the molecule in water, determined by mass spectrometry. Ethosome dispersion was thickened by poloxamer 407, obtaining an ethosomal gel that was characterized for rheological behavior and deformability. Caffeic acid diffusion kinetics were determined by Franz cells, while its penetration through skin, as well as its antioxidant activity, were evaluated using a porcine skin membrane–covered biosensor based on oxygen electrode. Ethosome mean diameter was ≈200 nm and almost stable within three months. The entrapment of caffeic acid in ethosome dramatically prolonged drug stability with respect to the aqueous solution, being 77% w/w in ethosome after six months, while in water, an almost complete degradation occurred within one month. The addition of poloxamer slightly modified vesicle structure and size, while it decreased the vesicle deformability. Caffeic acid diffusion coefficients from ethosome and ethosome gel were, respectively, 137- and 33-fold lower with respect to the aqueous solution. At last, the caffeic acid permeation and antioxidant power of ethosome were more intense with respect to the simple solution.
Identification and Pharmaceutical Characterization of a New Itraconazole Terephthalic Acid Cocrystal
Authors: Machado Cruz Ricardo , Boleslavská Tereza , Beránek Josef , Tieger Eszter , Twamley Brendan , Santos-Martinez Maria Jose , Dammer Ondřej , Tajber Lidia
Published: August 2020

The crystallization of poorly soluble drug molecules with an excipient into new solid phases called cocrystals has gained a considerable popularity in the pharmaceutical field. In this work, the cocrystal approach was explored for a very poorly water soluble antifungal active, itraconazole (ITR), which was, for the first time, successfully converted into this multicomponent solid using an aromatic coformer, terephthalic acid (TER). The new cocrystal was characterized in terms of its solid-state and structural properties, and a panel of pharmaceutical tests including wettability and dissolution were performed. Evidence of the cocrystal formation was obtained from liquid-assisted grinding, but not neat grinding. An efficient method of the ITR–TER cocrystal formation was ball milling. The stoichiometry of the ITR–TER phase was 2:1 and the structure was stabilized by H-bonds. When comparing ITR–TER with other cocrystals, the intrinsic dissolution rates and powder dissolution profiles correlated with the aqueous solubility of the coformers. The rank order of the dissolution rates of the active pharmaceutical ingredient (API) from the cocrystals was ITR–oxalic acid > ITR–succinic acid > ITR–TER. Additionally, the ITR–TER cocrystal was stable in aqueous conditions and did not transform to the parent drug. In summary, this work presents another cocrystal of ITR that might be of use in pharmaceutical formulations.
Robust Conformal Perfect Absorber Involving Lossy Ultrathin Film
Authors: Zhang Lei , Wang Kun , Chen Hui , Zhang Yanpeng
Published: August 2020

Perfect absorbers have been extensively investigated due to their significant value in solar cell, photodetection, and stealth technologies. Various subwavelength structures have been proposed to improve the absorption performances, such as high absorptance, broad band, and wide absorption angle. However, excellent performances usually put forward higher requirements on structural designs, such as varying the geometry sizes or shapes to fit different center wavelengths, which inevitably increases the fabrication burden. Here, a planar sandwich structure involving a layer of highly lossy material is proposed to achieve a robust perfect absorption with 95% absorptance ranging from the visible to near infrared range. Such an excellent absorption performance is also polarization-independent and applicable to a wide incident angle. Furthermore, the proposed design can also be applied to conformal surfaces with a 90% fluctuation over a steep surface. We believe that the proposed perfect absorber with distinguished performances can find wide application.
Assessing the Effect of Tandem Phase Sentinel-3 OLCI Sensor Uncertainty on the Estimation of Potential Ocean Chlorophyll-a Trends
Authors: Hammond Matthew L. , Henson Stephanie A. , Lamquin Nicolas , Clerc Sébastien , Donlon Craig
Published: August 2020

The Sentinel-3 tandem project represents the first time that two ocean colour satellites have been flown in the same orbit with minimal temporal separation (~30 s), thus allowing them to have virtually identical views of the ocean. This offers an opportunity for understanding how differences in individual sensor uncertainty can affect conclusions drawn from the data. Here, we specifically focus on trend estimation. Observational chlorophyll-a uncertainty is assessed from the Sentinel-3A Ocean and Land Colour Imager (OLCI-A) and Sentinel-3B OLCI (OLCI-B) sensors using a bootstrapping approach. Realistic trends are then imposed on a synthetic chlorophyll-a time series to understand how sensor uncertainty could affect potential long-term trends in Sentinel-3 OLCI data. We find that OLCI-A and OLCI-B both show very similar trends, with the OLCI-B trend estimates tending to have a slightly wider distribution, although not statistically different from the OLCI-A distribution. The spatial pattern of trend estimates is also assessed, showing that the probability distributions of trend estimates in OLCI-A and OLCI-B are most similar in open ocean regions, and least similar in coastal regions and at high northern latitudes. This analysis shows that the two sensors should provide consistent trends between the two satellites, provided future ageing is well quantified and mitigated. The Sentinel-3 programme offers a strong baseline for estimating long-term chlorophyll-a trends by offering a series of satellites (starting with Sentinel-3A and Sentinel-3B) that use the same sensor design, reducing potential issues with cross-calibration between sensors. This analysis contributes an important understanding of the reliability of the two current Sentinel-3 OLCI sensors for future studies of climate change driven chlorophyll-a trends.
MODIS and VIIRS Calibration History and Future Outlook
Authors: Xiong Xiaoxiong , Butler James J.
Published: August 2020

The MODIS is a key instrument for NASA’s EOS program, currently operated onboard the Terra and Aqua spacecraft launched in 1999 and 2002, respectively. The VIIRS is a MODIS follow-on instrument for the JPSS program. Adding to the ones operated onboard the S-NPP and NOAA-20 satellites launched in 2011 and 2017, respectively, three nearly identical VIIRS instruments will also be launched. This will enable the data records from MODIS and VIIRS to be extended beyond 2040. In addition to various applications and scientific studies of the Earth’s system, long-term data records from MODIS and VIIRS observations will greatly benefit the space-based climate observing system. This is attributed to the high-quality measurements and extensive calibration efforts, from pre-launch to post-launch. This paper provides an overview of MODIS and VIIRS calibration history and approaches applied to establish and maintain sensor calibration traceability and accuracy. It illustrates calibration and performance issues through different phases of the mission using examples derived from ground testing equipment, on-board calibrators, and other calibration targets. Moreover, discussed in this paper are outstanding challenges and future efforts to maintain and improve sensor calibration stability and long-term data quality, and to better support the space-based climate observing system.
Methods of Rapid Quality Assessment for National-Scale Land Surface Change Monitoring
Authors: Zhou Qiang , Barber Christopher , Xian George
Published: August 2020

Providing rapid access to land surface change data and information is a goal of the U.S. Geological Survey. Through the Land Change Monitoring, Assessment, and Projection (LCMAP) initiative, we have initiated a monitoring capability that involves generating a suite of 10 annual land cover and land surface change datasets across the United States at a 30-m spatial resolution. During the LCMAP automated production, on a tile-by-tile basis, erroneous data can occasionally be generated due to hardware or software failure. While crucial to assure the quality of the data, rapid evaluation of results at the pixel level during production is a substantial challenge because of the massive data volumes. Traditionally, product quality relies on the validation after production, which is inefficient to reproduce the whole product when an error occurs. This paper presents a method for automatically evaluating LCMAP results during the production phase based on 14 indices to quickly find and flag erroneous tiles in the LCMAP products. The methods involved two types of comparisons: comparing LCMAP values across the temporal record to measure internal consistency and calculating the agreement with multiple intervals of the National Land Cover Database (NLCD) data to measure the consistency with existing products. We developed indices on a tile-by-tile basis in order to quickly find and flag potential erroneous tiles by comparing with surrounding tiles using local outlier factor analysis. The analysis integrates all indices into a local outlier score (LOS) to detect erroneous tiles that are distinct from neighboring tiles. Our analysis showed that the methods were sensitive to partially erroneous tiles in the simulated data with a LOS higher than 2. The rapid quality assessment methods also successfully identified erroneous tiles during the LCMAP production, in which land surface change results were not properly saved to the products. The LOS map and indices for rapid quality assessment also point to directions for further investigations. A map of all LOS values by tile for the published LCMAP shows all LOS values are below 2. We also investigated tiles with high LOS to ensure the distinction with neighboring tiles was reasonable. An index in this study shows the overall agreement between LCMAP and NLCD on a tile basis is above 71.5% and has an average at 89.1% across the 422 tiles in the conterminous United States. The workflow is suitable for other studies with a large volume of image products.
Model-Based Estimation of Forest Inventory Attributes Using Lidar: A Comparison of the Area-Based and Semi-Individual Tree Crown Approaches
Authors: Frank Bryce , Mauro Francisco , Temesgen Hailemariam
Published: August 2020

The use of individual tree detection methods to support forest management inventories has been a research topic for over two decades, but a formal assessment of these methods to produce stand-level and region-level predictions of forest attributes and measures of error is lacking. We employed model-based estimation methods in conjunction with the semi-individual tree crown approach (s-ITC) to produce predictions and measures of error for tree volume (VOL), basal area (BA), stem density (DEN), and quadratic mean diameter (QMD) at the scale of forest stands and the entire study region. We compared the s-ITC approach against the area-based approach (ABA) for predictions of region-level and stand-level attributes via model-based root mean squared errors (RMSEs). The study was conducted at the Panther Creek watershed in Oregon, USA using a set of 78 field plots and aerial lidar information. For region-level attributes, s-ITC RMSEs demonstrated changes between −31% and 17% relative to ABA models. At the stand level, median s-ITC RMSEs generally increased, with changes between −29% and 414% relative to ABA models, but demonstrated important reductions in stands where segmentation provided large increases in sample size and was less prone to extrapolation than ABA models. The ABA demonstrated smaller RMSEs in stands without sampled population units for all variables. Our findings motivate further research into niche applications where s-ITC models may consistently outperform ABA models.