bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Characteristics and Contemporary Prognosis of Ventricular Septal Rupture Complicating Acute Myocardial Infarction: A Single-Center Experience
Authors: Wang Lu , Xiao Li-Li , Liu Chao , Zhang Yan-Zhou , Zhao Xiao-Yan , Li Ling , Wang Xiao-Fang , Dong Jian-Zeng
Published: September 2021

Objectives: Ventricular septal rupture (VSR) is a rare but lethal complication of acute myocardial infarction (AMI). We conducted a retrospective analysis of the clinical characteristics of VSR patients and explored the risk factors for long-term mortality.

Methods: In this single-center cohort study, 127 patients diagnosed with post-AMI VSR between May 2012 and April 2019 were included. Demographic, clinical, operative, and outcome data were collected. The 30-day and long-term mortality were outcomes of interest. Cox proportional hazard regression analysis was used to explore the predictors of long-term mortality.

Results: The mean age of the VSR cohort was 66.6 ± 8.7 years, 67 (52.8%) were males. Among the 127 patients, 78 patients (61.4%) were medically managed, 31 (24.4%) patients underwent percutaneous transcatheter closure (TCC), and 18 (14.2%) patients received surgical repair. The median follow-up time was 1129 days [interquartile range: 802–2019 days]. The 30-day mortality of the medically managed group, percutaneous TCC group, and surgical management group was 93.6, 22.6, and 11.1%, respectively; and the long-term mortality was 96.2, 25.8, and 22.2%, respectively. VSR repair treatment including surgical management (HR 0.01, 95% CI 0.001–0.09, p < 0.001) and percutaneous TCC (HR 0.09, 95% CI 0.03–0.26, p < 0.001) was associated with a better prognosis, and cardiogenic shock (CS) (HR 9.30, 95% CI 3.38–25.62, p < 0.001) was an independent risk factor of long-term mortality.

Conclusions: The prognosis of VSR patients without operative management remains poor, especially in those complicated with CS. Timely and improved surgery treatment is needed for better outcomes in VSR patients.
Long-Term Stability and Biocompatibility of Pericardial Bioprosthetic Heart Valves
Authors: Williams David F. , Bezuidenhout Deon , de Villiers Jandre , Human Paul , Zilla Peter
Published: September 2021

The use of bioprostheses for heart valve therapy has gradually evolved over several decades and both surgical and transcatheter devices are now highly successful. The rapid expansion of the transcatheter concept has clearly placed a significant onus on the need for improved production methods, particularly the pre-treatment of bovine pericardium. Two of the difficulties associated with the biocompatibility of bioprosthetic valves are the possibilities of immune responses and calcification, which have led to either catastrophic failure or slow dystrophic changes. These have been addressed by evolutionary trends in cross-linking and decellularization techniques and, over the last two decades, the improvements have resulted in somewhat greater durability. However, as the need to consider the use of bioprosthetic valves in younger patients has become an important clinical and sociological issue, the requirement for even greater longevity and safety is now paramount. This is especially true with respect to potential therapies for young people who are afflicted by rheumatic heart disease, mostly in low- to middle-income countries, for whom no clinically acceptable and cost-effective treatments currently exist. To extend longevity to this new level, it has been necessary to evaluate the mechanisms of pericardium biocompatibility, with special emphasis on the interplay between cross-linking, decellularization and anti-immunogenicity processes. These mechanisms are reviewed in this paper. On the basis of a better understanding of these mechanisms, a few alternative treatment protocols have been developed in the last few years. The most promising protocol here is based on a carefully designed combination of phases of tissue-protective decellularization with a finely-titrated cross-linking sequence. Such refined protocols offer considerable potential in the progress toward superior longevity of pericardial heart valves and introduce a scientific dimension beyond the largely disappointing ‘anti-calcification’ treatments of past decades.
Numerical Simulation Study of Tracking the Displacement Fronts and Enhancing Oil Recovery Based on Ferrofluid Flooding
Authors: Huang Tao , Song Fuquan , Wang Renyi , Huang Xiaohe
Published: September 2021

Water flooding is crucial means to improve oil recovery after primary production. However, the utilization ratio of injected water is often seriously affected by heterogeneities in the reservoir. Identification of the location of the displacement fronts and the associated reservoir heterogeneity is important for the management and improvement of water flooding. In recent years, ferrofluids have generated much interest from the oil industry owning to its unique properties. First, saturation of ferrofluids alters the magnetic permeability of the porous medium, which means that the presence of ferrofluids should produce magnetic anomalies in an externally imposed magnetic field or the local geomagnetic field. Second, with a strong external magnetic field, ferrofluids can be guided into regions that were bypassed and with high residual oil saturation. In view of these properties, a potential dual-application of ferrofluid as both a tracer to locate the displacement front and a displacing fluid to improve recovery in a heterogeneous reservoir is examined in this paper. Throughout the injection process, the magnetic field generated by electromagnets and altered by the distribution of ferrofluids was calculated dynamically by applying a finite element method, and a finite volume method was used to solve the multiphase flow. Numerical simulation results indicate that the displacement fronts in reservoirs can indeed be detected, through which the major features of reservoir heterogeneity can be inferred. After the locations of the displacement fronts and reservoir heterogeneities are identified, strong magnetic fields were applied to direct ferrofluids into poorly swept regions and the efficiency of the flooding was significantly improved.
Signatures and Clinical Significance of Amino Acid Flux in Sarcopenia: A Systematic Review and Meta-Analysis
Authors: Dai Miao , Lin Taiping , Yue Jirong , Dai Lunzhi
Published: September 2021

Background

Dysregulation of amino acids is closely linked to the initiation and progression of sarcopenia. We summarized recent advancements in the studies of amino acid profiles in sarcopenia and systematically presented the clinical significance of amino acid flux in sarcopenia.

Methods

We systematically searched in MEDLINE, EMBASE, and Cochrane library from inception to June 1, 2021 to capture all studies examining metabolomics of sarcopenia. We used the following keywords: sarcopenia, metabonomics, metabolomics, amino acid profile, and mass spectrometry. Original articles comparing amino acid patterns between persons with and without sarcopenia were included. Two independent investigators independently completed title and abstract screening, data extraction, and quality evaluation. We used a random effects model to examine the association between amino acids levels and sarcopenia. Sensitivity analyses restricted the analyses to studies in which muscle mass was measured by bioelectrical impedance analysis. Study quality was evaluated according to the Agency for Healthcare Research and Quality (AHRQ) checklist.

Results

The systematic research yielded six eligible articles, comprising 1,120 participants. Five studies used muscle mass in combination with physical performance and/or muscle strength as the criteria to diagnose sarcopenia, while one study used muscle mass as a diagnostic criterion alone. We found that the concentrations of branched-chain amino acids leucine (standardized mean difference [SMD] -1.249; 95% confidence interval [CI]: -2.275, -0.223, P = 0.02, I2 = 97.7%), isoleucine (SMD -1.077; 95% CI: -2.106, -0.049, P = 0.04, I2 = 97.8%), and aromatic amino acid tryptophan (SMD -0.923; 95% CI: -1.580, -0.265, P = 0.01, I2 = 89.9%) were significantly reduced in individuals with sarcopenia. Study results were robust in sensitivity analysis.

Conclusions

The homeostasis of amino acids is critical to maintaining muscle health. The profiles of amino acids might be useful biomarkers for the characterization of sarcopenia. Future studies are warranted to study the clinical significance of amino acids in the diagnosis and treatment of sarcopenia.
Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes
Authors: Cayabyab Fritz , Nih Lina R. , Yoshihara Eiji
Published: September 2021

Diabetes is a complex disease that affects over 400 million people worldwide. The life-long insulin injections and continuous blood glucose monitoring required in type 1 diabetes (T1D) represent a tremendous clinical and economic burdens that urges the need for a medical solution. Pancreatic islet transplantation holds great promise in the treatment of T1D; however, the difficulty in regulating post-transplantation immune reactions to avoid both allogenic and autoimmune graft rejection represent a bottleneck in the field of islet transplantation. Cell replacement strategies have been performed in hepatic, intramuscular, omentum, and subcutaneous sites, and have been performed in both animal models and human patients. However more optimal transplantation sites and methods of improving islet graft survival are needed to successfully translate these studies to a clinical relevant therapy. In this review, we summarize the current progress in the field as well as methods and sites of islet transplantation, including stem cell-derived functional human islets. We also discuss the contribution of immune cells, vessel formation, extracellular matrix, and nutritional supply on islet graft survival. Developing new transplantation sites with emerging technologies to improve islet graft survival and simplify immune regulation will greatly benefit the future success of islet cell therapy in the treatment of diabetes.
V2G Multi-Objective Dispatching Optimization Strategy Based on User Behavior Model
Authors: Li Tianyu , Tao Shengyu , He Kun , Lu Mengke , Xie Binglei , Yang Biao , Sun Yaojie
Published: September 2021

V2G (Vehicle to Grid) technology can adjust the grid load through the unified control of the charging and discharging of electric vehicles (EVs), and achieve peak shaving and valley filling to smooth load fluctuations. Aiming at the random and uncertain problem of EV users travel and behavior decision-making, this paper proposes a V2G multi-objective dispatching strategy based on user behavior. First, a V2G behavior model was established based on user behavior questionnaire surveys, and the effective effect of EV load was simulated through Monte Carlo simulation. Then, combined with the regional daily load curve and peak-valley time-of-use electricity prices, with the goal of stabilizing grid load fluctuations and increasing the benefits of EV users, a multi-objective optimal dispatching model for EV clusters charging and discharging is established. Finally, Considering the needs of EV users and the operation constraints of the microgrid, the genetic algorithm is used to obtain the Pareto optimal solution. The results show that when dispatching with the maximum benefit of users, the peak-to-valley ratio of the grid side can be reduced by 2.99%, and the variance can be reduced by 9.52%. The optimization strategy can use peak and valley time-of-use electricity prices to guide the intelligent charging and discharging of EVs while meeting user needs, so as to achieve the optimal multi-objective benefit of V2G participation in power response.
Single-Ended Fast Protection for Transient Power of Multi-Terminal Flexible and Direct Power Grids Containing Renewable Energy
Authors: Shan Jieshan , Tian Xincui , Dai Yue , Shu Hongchun , Chang Yong , Song Jian
Published: September 2021

With the proposal of the carbon peak goal, a multi-terminal flexible DC grid containing various renewable energy becomes the one of main ways of renewable energy power transmission. Thus, it is increasingly important to improve its transmission reliability and stability. This paper proposes a single-ended protection principle based on the analysis method of transient power change for the “mesh structure” ring-shaped flexible direct current (DC) grid. Based on the propagation characteristics of voltage, current, and anti-traveling waves during fault periods, the transient power variation coefficient based on SOD transformation is introduced to distinguish the internal and external faults. Besides, simulation based on the PSCAD/EMTDC platform within the Zhangbei four-port network verifies the effectiveness of the proposed protection principle under various fault conditions. Meanwhile, the protection criterion is designed according to the difference in the change coefficient of the transient power SOD within and outside the fault time zone. Moreover, the setting value of the start of protection is obtained by analyzing the simulation results, thus a set of fast and reliable single-ended protections for the same-side short-time transient power window of an enhanced flexible straight power grid is developed via combining it with the starting criterion of a sudden voltage change.
Whole-Genome Methylation Analysis Revealed ART-Specific DNA Methylation Pattern of Neuro- and Immune-System Pathways in Chinese Human Neonates
Authors: Liu Zongzhi , Chen Wei , Zhang Zilong , Wang Junyun , Yang Yi-Kun , Hai Luo , Wei Yuan , Qiao Jie , Sun Yingli
Published: September 2021

The DNA methylation of human offspring can change due to the use of assisted reproductive technology (ART). In order to find the differentially methylated regions (DMRs) in ART newborns, cord blood maternal cell contamination and parent DNA methylation background, which will add noise to the real difference, must be removed. We analyzed newborns’ heel blood from six families to identify the DMRs between ART and natural pregnancy newborns, and the genetic model of methylation was explored, meanwhile we analyzed 32 samples of umbilical cord blood of infants born with ART and those of normal pregnancy to confirm which differences are consistent with cord blood data. The DNA methylation level was lower in ART-assisted offspring at the whole genome-wide level. Differentially methylated sites, DMRs, and cord blood differentially expressed genes were enriched in the important pathways of the immune system and nervous system, the genetic patterns of DNA methylation could be changed in the ART group. A total of three imprinted genes and 28 housekeeping genes which were involved in the nervous and immune systems were significant different between the two groups, six of them were detected both in heel blood and cord blood. We concluded that there is an ART-specific DNA methylation pattern involved in neuro- and immune-system pathways of human ART neonates, providing an epigenetic basis for the potential long-term health risks in ART-conceived neonates.
Identification and Elucidation of the Protective isomiRs in Lung Cancer Patient Prognosis
Authors: Hsieh Fu-Mei , Lai Su-Ting , Wu Ming-Fong , Lin Chen-Ching
Published: September 2021

MicroRNAs (miRNAs) are approximately 20–22 nucleotides in length, which are well known to participate in the post-transcriptional modification. The mature miRNAs were observed to be varied on 5′ or 3′ that raise another term—the isoforms of mature miRNAs (isomiRs), which have been proven not the artifacts and discussed widely recently. In our research, we focused on studying the 5′ isomiRs in lung adenocarcinoma (LUAD) in The Cancer Genome Atlas (TCGA). We characterized 75 isomiRs significantly associated with better prognosis and 43 isomiRs with poor prognosis. The 75 protective isomiRs can successfully distinguish tumors from normal samples and are expressed differently between patients of early and late stages. We also found that most of the protective isomiRs tend to be with downstream shift and upregulated compared with those with upstream shift, implying that a possible selection occurs during cancer development. Among these protective isomiRs, we observed a highly positive and significant correlation, as well as in harmful isomiRs, suggesting cooperation within the group. However, between protective and harmful, there is no such a concordance but conversely more negative correlation, suggesting the possible antagonistic effect between protective and harmful isomiRs. We also identified that two isomiRs miR-181a-3p|-3 and miR-181a-3p|2, respectively, belong to the harmful and protective groups, suggesting a bidirectional regulation of their originated archetype—miR-181a-3p. Additionally, we found that the protective isomiRs of miR-21-5p, which is an oncomiR, may be evolved as the tumor suppressors through producing isomiRs to hinder metastasis. In summary, these results displayed the characteristics of the protective isomiRs and their potential for developing the treatment of lung cancer.
Identification of Prognostic Biomarker Candidates Associated With Melanoma Using High-Dimensional Genomic Data
Authors: Kutt Brody , Burdorf Rachel , Bain Travaughn , Cameron Nicardo , Pearah Alexia , Subasi Ersoy , Carroll David J. , Moore Lisa K. , Subasi Munevver Mine
Published: September 2021

Survival of patients with metastatic melanoma varies widely. Melanoma is a highly proliferative, chemo-resistant disease. With the recent availability of immunotherapies such as checkpoint inhibitors, durable response rates have improved but are often still limited to 2–3 years. Response rates to treatment range from 30 to 45% with combination therapy however no improvement in overall survival is frequently observed. Of the available therapies, many have targeted the BRAFV600E mutation that results in abnormal MAPK pathway activation which is important for regulating cell proliferation. Immune checkpoint inhibitors such as anti-PD-1 and anti-PD-L1 offer better success but response rates are still low. Identifying biomarkers to better target those who will respond and identify the right combination of treatment is the best approach. In this study, we utilize data from the Cancer Cell Line Encyclopedia (CCLE), including 62 samples, to examine features of gene expression (19K+) and copy number (20K+) in the melanoma cell lines. We perform a clustering analysis on the feature set to assess genetically similarity among the cell lines. We then discover which specific genes and combinations thereof maximize cluster density. We design a feature selection approach for high-dimensional datasets that integrates multiple disparate machine learning techniques into one cohesive pipeline. Our approach provides a small subset of genes that can accurately distinguish between the clusters of melanoma cell lines across multiple types of classifiers. In particular, we find only the 15 highest ranked genes among the original 19 K are necessary to achieve perfect or near-perfect test split classification performance. Of these 15 genes, some are known to be linked to melanoma or other cancer progressions, while others have not previously been linked to melanoma and are of interest for further examination.
Novel MYO1D Missense Variant Identified Through Whole Exome Sequencing and Computational Biology Analysis Expands the Spectrum of Causal Genes of Laterality Defects
Authors: Alsafwani Rabab Said , Nasser Khalidah K. , Shinawi Thoraia , Banaganapalli Babajan , ElSokary Hanan Abdelhalim , Zaher Zhaher F. , Shaik Noor Ahmad , Abdelmohsen Gaser , Al-Aama Jumana Yousuf , Shapiro Adam J. , O. Al-Radi Osman , Elango Ramu , Alahmadi Turki
Published: September 2021

Laterality defects (LDs) or asymmetrically positioned organs are a group of rare developmental disorders caused by environmental and/or genetic factors. However, the exact molecular pathophysiology of LD is not yet fully characterised. In this context, studying Arab population presents an ideal opportunity to discover the novel molecular basis of diseases owing to the high rate of consanguinity and genetic disorders. Therefore, in the present study, we studied the molecular basis of LD in Arab patients, using next-generation sequencing method. We discovered an extremely rare novel missense variant in MYO1D gene (Pro765Ser) presenting with visceral heterotaxy and left isomerism with polysplenia syndrome. The proband in this index family has inherited this homozygous variant from her heterozygous parents following the autosomal recessive pattern. This is the first report to show MYO1D genetic variant causing left–right axis defects in humans, besides previous known evidence from zebrafish, frog and Drosophila models. Moreover, our multilevel bioinformatics-based structural (protein variant structural modelling, divergence, and stability) analysis has suggested that Ser765 causes minor structural drifts and stability changes, potentially affecting the biophysical and functional properties of MYO1D protein like calmodulin binding and microfilament motor activities. Functional bioinformatics analysis has shown that MYO1D is ubiquitously expressed across several human tissues and is reported to induce severe phenotypes in knockout mouse models. In conclusion, our findings show the expanded genetic spectrum of LD, which could potentially pave way for the novel drug target identification and development of personalised medicine for high-risk families.
COVID-19 Seroprevalence and Active Infection in an Asymptomatic Population
Authors: Breedon Amy M. E. , Saldanha Roland J. , Salisbury Richard L. , Metzger David E. , Werry Michael P. , McPherson Craig J. , Irvin Adam P. , Davis Christina M. , Bogner Charles A. , Braddock Amber M. , Salter Charles E. , Grigsby Claude C. , Hart Corey R. , Pangburn Heather A.
Published: September 2021

In response to the COVID-19 pandemic, immediate and scalable testing solutions are needed to direct return to full capacity planning in the general public and across the Department of Defense (DoD). To fully understand the extent to which a population has been affected by COVID-19, active monitoring approaches require an estimation of overall seroprevalence in addition to accurate, affordable, and rapid tests to detect current SARS-CoV-2 infection. In this study, researchers in the Air Force Research Laboratory's 711th Human Performance Wing, Airman Systems Directorate evaluated the performance of various testing methods for the detection of SARS-CoV-2 antibodies and viral RNA in asymptomatic adults working at Wright-Patterson Air Force Base and the surrounding area during the period of 23 July 2020–23 Oct 2020. Altogether, there was a seroprevalance of 3.09% and an active infection rate of 0.5% (determined via the testing of saliva samples) amongst individuals tested, both of which were comparable to local and national averages at the time. This work also presents technical and non-technical assessments of various testing strategies as compared to the gold standard approaches (e.g., lateral flow assays vs. ELISA and RT-LAMP vs. RT-PCR) in order to explore orthogonal supply chains and fieldability. Exploration and validation of multiple testing strategies will allow the DoD and other workforces to make informed responses to COVID-19 and future pandemics.
Planktonic Archaeal Ether Lipid Origins in Surface Waters of the North Pacific Subtropical Gyre
Authors: Li Fuyan , Leu Andy , Poff Kirsten , Carlson Laura T. , Ingalls Anitra E. , DeLong Edward F.
Published: September 2021

Thaumarchaeota and Thermoplasmatota are the most abundant planktonic archaea in the sea. Thaumarchaeota contain tetraether lipids as their major membrane lipids, but the lipid composition of uncultured planktonic Thermoplasmatota representatives remains unknown. To address this knowledge gap, we quantified archaeal cells and ether lipids in open ocean depth profiles (0–200 m) of the North Pacific Subtropical Gyre. Planktonic archaeal community structure and ether lipid composition in the water column partitioned into two separate clusters: one above the deep chlorophyll maximum, the other within and below it. In surface waters, Thermoplasmatota densities ranged from 2.11 × 106 to 6.02 × 106 cells/L, while Thaumarchaeota were undetectable. As previously reported for Thaumarchaeota, potential homologs of archaeal tetraether ring synthases were present in planktonic Thermoplasmatota metagenomes. Despite the absence of Thaumarchaeota in surface waters, measurable amounts of intact polar ether lipids were found there. Based on cell abundance estimates, these surface water archaeal ether lipids contributed only 1.21 × 10–9 ng lipid/Thermoplasmatota cell, about three orders of magnitude less than that reported for Thaumarchaeota cells. While these data indicate that even if some tetraether and diether lipids may be derived from Thermoplasmatota, they would only comprise a small fraction of Thermoplasmatota total biomass. Therefore, while both MGI Thaumarchaeota and MGII/III Thermoplasmatota are potential biological sources of archaeal GDGTs, the Thaumarchaeota appear to be the major contributors of archaeal tetraether lipids in planktonic marine habitats. These results extend and confirm previous reports of planktonic archaeal lipid sources, and further emphasize the need for Thermoplasmatota cultivation, to better characterize the membrane lipid constituents of marine planktonic Thermoplasmatota, and more precisely define the sources and patterns of archaeal tetraether lipid distributions in marine plankton.
Whole Genome Sequence-Based Identification of Clostridium estertheticum Complex Strains Supports the Need for Taxonomic Reclassification Within the Species Clostridium estertheticum
Authors: Wambui Joseph , Cernela Nicole , Stevens Marc J. A. , Stephan Roger
Published: September 2021

Isolates within the Clostridium estertheticum complex (CEC) have routinely been identified through the 16S rRNA sequence, but the high interspecies sequence similarity reduces the resolution necessary for species level identification and often results in ambiguous taxonomic classification. The current study identified CEC isolates from meat juice (MJS) and bovine fecal samples (BFS) and determined the phylogeny of species within the CEC through whole genome sequence (WGS)-based analyses. About 1,054 MJS were screened for CEC using quantitative real-time PCR (qPCR). Strains were isolated from 33 MJS and 34 BFS qPCR-positive samples, respectively. Pan- and core-genome phylogenomics were used to determine the species identity of the isolates. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) were used to validate the species identity. The phylogeny of species within the CEC was determined through a combination of these methods. Twenty-eight clostridia strains were isolated from MJS and BFS samples out of which 13 belonged to CEC. At 95% ANI and 70% dDDH thresholds for speciation, six CEC isolates were identified as genomospecies2 (n=3), Clostridium tagluense (n=2) and genomospecies3 (n=1). Lower thresholds of 94% ANI and 58% dDDH were required for the classification of seven CEC isolates into species C. estertheticum and prevent an overlap between species C. estertheticum and Clostridium frigoriphilum. Combination of the two species and abolishment of current subspecies classification within the species C. estertheticum are proposed. These data demonstrate the suitability of phylogenomics to identify CEC isolates and determine the phylogeny within CEC.
The Engram’s Dark Horse: How Interneurons Regulate State-Dependent Memory Processing and Plasticity
Authors: Raven Frank , Aton Sara J.
Published: September 2021

Brain states such as arousal and sleep play critical roles in memory encoding, storage, and recall. Recent studies have highlighted the role of engram neurons–populations of neurons activated during learning–in subsequent memory consolidation and recall. These engram populations are generally assumed to be glutamatergic, and the vast majority of data regarding the function of engram neurons have focused on glutamatergic pyramidal or granule cell populations in either the hippocampus, amygdala, or neocortex. Recent data suggest that sleep and wake states differentially regulate the activity and temporal dynamics of engram neurons. Two potential mechanisms for this regulation are either via direct regulation of glutamatergic engram neuron excitability and firing, or via state-dependent effects on interneuron populations–which in turn modulate the activity of glutamatergic engram neurons. Here, we will discuss recent findings related to the roles of interneurons in state-regulated memory processes and synaptic plasticity, and the potential therapeutic implications of understanding these mechanisms.
Music Augmented With Isochronic Auditory Beats or Vibrotactile Stimulation Does Not Affect Subsequent Ergometer Cycling Performance: A Pilot Study
Authors: Fry Adam , Braren Stephen , Pitaro Nicholas , Larson Brandon , Putrino David
Published: September 2021

Methods to enhance the ergogenic effects of music are of interest to athletes of all abilities. The aim of this pilot study was to investigate the ergogenic effects of two commercially available methods of music augmentation: auditory beats and vibrotactile stimulation. Six male and five female cyclists/triathletes cycled for 7 minutes at three different intensities: a rate of perceived exertion (RPE) of 11 (“light”), RPE of 15 (“hard”), and a 7-minute time-trial. Before each 7-minute bout of cycling, participants listened to 10 minutes of self-selected music (MUS), or the same music with the addition of either isochronic auditory beats (ABS) or vibrotactile stimulation via SUBPACTM(VIB). MUS, ABS and VIB trials were performed in a randomized order. Power output was measured during cycling and felt arousal and feeling scores were recorded at timepoints throughout the protocol. The results found the augmented MUS interventions did not influence power output with no significant main effect of trial (p= 0.44, η2= 0.09) or trial × cycling intensity interaction (p= 0.11, η2= 0.20). Similarly, both felt arousal and feeling scores were unchanged between the MUS, ABS, and VIB trials (p> 0.05). In conclusion, this pilot study indicated an ineffectiveness of the ABS and VIB to affect subsequent 7-min cycling performance compared to self-selected MUS alone.
The Effect of Yoga Meditation Practice on Young Adults’ Inhibitory Control: An fNIRS Study
Authors: Jiang Dongdong , Liu Zongyu , Sun Guoxiao
Published: September 2021

Objectives: The present study aimed to test the effect of yoga meditation (YoMed) practice on inhibitory control of young adults.

Methods: A total of 50 participants (23 male, 21–28 years old) from a university in Jinan, Shandong Province were enrolled in this study. Participants were randomly assigned to a YoMed group or a Control group. Participants’ basic information, physical activity, and inhibitory control were measured. A multi-channel continuous-wave near-infrared spectrometer was used to monitor the brain’s hemodynamic responses.

Results: After the intervention, we found significant differences in Flanker tasks between the YoMed group and Control group. The accuracy in the YoMed group was higher than those in the Control group (p < 0.05). Analysis of fNIRS data showed that oxyhemoglobin (oxy-Hb) levels in the prefrontal cortex (PFC) increased in the YoMed group during the Flanker tasks after the YoMed intervention.

Conclusion: YoMed has a temporarily promoting effect on the brain activation of young adults. It is an effective and appropriate exercise to improve the inhibitory control of young adults.
Multicentre Comparison of the Toxicity and Effectiveness of Lobaplatin-Based Versus Cisplatin-Based Adjuvant Chemotherapy in Oesophageal Carcinoma
Authors: Zheng Yan , Li Yin , Liu Xianben , Sun Haibo , Liang Guanghui , Hu Jiajia , Li Liping , Xing Wenqun
Published: September 2021

Objectives

Lobaplatin (LBP), a third-generation cisplatin derivative has shown promising activity and few side effects in oesophageal squamous cell carcinoma (ESCC) in previous reports. We compared LBP plus docetaxel with cisplatin plus docetaxel as adjuvant chemotherapy in ESCC patients to determine the effects on overall survival (OS) and toxicity.

Methods

A multicentre retrospective study was performed using propensity score matching (PSM) with the Medicine-LinkDoc database. Patients diagnosed with stage II-III ESCC treated with adjuvant chemotherapy (cisplatin plus docetaxel or LBP plus docetaxel) between January 2013 and December 2016 were selected from 6 centres in China.

Results

There were 733 eligible ESCC patients. After PSM (1:1 ratio), 458 patients remained. The 5-year OS rates of the cisplatin and LBP groups were 25.9% and 23.6%, respectively (P=0.457). Leukopenia (grade III-IV/I-II/0: 2.62%/34.5%/59.39% versus 5.24%/43.23%/45.85%; P=0.0176), neutropenia (grade III-IV/I-II/0: 6.55%/37.56%/51.09% versus 4.37%/53.28%/36.34%; P=0.0015), nephrotoxicity (grade I-II/0: 13.97%/76.86% versus 26.64%/65.94%; P<0.001) and gastrointestinal symptoms (grade III-IV/I-II/0: 2.18%/54.59%/32.31% versus 6.55%/65.07%/20.88%; P=0.0011) were more frequent in the cisplatin group.

Conclusions

Compared with cisplatin plus docetaxel, LBP plus docetaxel provided the same survival benefits but lower side effects of myelosuppression and gastrointestinal symptoms. LBP plus docetaxel might be a choice for adjuvant chemotherapy in ESCC.

Clinical Trial Registration

Lobaplatin or Cisplatin in Adjuvant Chemotherapy for Oesophageal Carcinoma, identifier NCT03413436.
The Role of Non-Coding RNAs in Breast Cancer Drug Resistance
Authors: Tian Jin-hai , Liu Shi-hai , Yu Chuan-yang , Wu Li-gang , Wang Li-bin
Published: September 2021

Breast cancer (BC) is one of the commonly occurring malignancies in females worldwide. Despite significant advances in therapeutics, the mortality and morbidity of BC still lead to low survival and poor prognosis due to the drug resistance. There are certain chemotherapeutic, endocrine, and target medicines often used for BC patients, including anthracyclines, taxanes, docetaxel, cisplatin, and fluorouracil. The drug resistance mechanisms of these medicines are complicated and have not been fully elucidated. It was reported that non-coding RNAs (ncRNAs), such as micro RNAs (miRNA), long-chain non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) performed key roles in regulating tumor development and mediating therapy resistance. However, the mechanism of these ncRNAs in BC chemotherapeutic, endocrine, and targeted drug resistance was different. This review aims to reveal the mechanism and potential functions of ncRNAs in BC drug resistance and to highlight the ncRNAs as a novel target for achieving improved treatment outcomes for BC patients.
Molecular Alterations in Lung Adenocarcinoma With Ground-Glass Nodules: A Systematic Review and Meta-Analysis
Authors: Wei Zihan , Wang Ziyang , Nie Yuntao , Zhang Kai , Shen Haifeng , Wang Xin , Wu Manqi , Yang Fan , Chen Kezhong
Published: September 2021

Background and Aims

Nodular ground-glass lesions have become increasingly common with the increased use of computed tomography (CT), while the genomic features of ground-glass opacities (GGOs) remain unclear. This study aims to comprehensively investigate the molecular alterations of GGOs and their correlation with radiological progression.

Methods

Studies from PubMed, Embase, Cochrane Library, and Web of Science, using PCR, targeted panel sequencing, whole exosome sequencing, and immunohistochemistry, and reporting genomic alterations or PD-L1 expressions in lung nodules presenting as GGOs until January 21, 2021 were included in this study. Chi-square test, random-effects model, and Z-test analysis were adopted to analyze the data.

Results

A total of 22 studies describing mutations in lung adenocarcinoma (LUAD) with GGOs were analyzed. EGFR was the most frequently mutative gene (51%, 95%CI 47%–56%), followed by TP53 (18%, 95%CI 6%–31%), HER2 (10%, 95%CI 0%–21%), ROS1 (6%, 95%CI 0%–18%), and KRAS (6%, 95%CI 3%–9%). The correlation between the frequency of EGFR mutation and radiological was observed and the differences were found to be not statistically significant in the subgroups, which are listed as below: radiological: gGGO 47.40%, 95%CI [38.48%; 56.40%]; sGGO 51.94%, 95%CI [45.15%; 58.69%]. The differences of the frequency of KRAS mutation in the different subgroups were also consistent with this conclusion, which are listed as: radiological gGGO 3.42, 95%CI [1.35%; 6.13%]; sGGO 12.27%, 95%CI [3.89%; 23.96%]. The pooled estimated rate of PD-L1 was 8.82%, 95%CI [5.20%–13.23%]. A total of 11.54% (3/26) of the SMGGNs were confirmed to be intrapulmonary spread by WES.

Conclusions

Somatic genetic alterations are considered in early-stage GGO patients without distinct changes of the frequency following the progress of the tumor. This review sheds insight on molecular alterations in LUAD with GGOs.
Modified Predictive Model and Nomogram by Incorporating Prebiopsy Biparametric Magnetic Resonance Imaging With Clinical Indicators for Prostate Biopsy Decision Making
Authors: Pan Jin-feng , Su Rui , Cao Jian-zhou , Zhao Zhen-ya , Ren Da-wei , Ye Sha-zhou , Huang Rui-da , Tao Zhu-lei , Yu Cheng-ling , Jiang Jun-hui , Ma Qi
Published: September 2021

Purpose

The purpose of this study is to explore the value of combining bpMRI and clinical indicators in the diagnosis of clinically significant prostate cancer (csPCa), and developing a prediction model and Nomogram to guide clinical decision-making.

Methods

We retrospectively analyzed 530 patients who underwent prostate biopsy due to elevated serum prostate specific antigen (PSA) levels and/or suspicious digital rectal examination (DRE). Enrolled patients were randomly assigned to the training group (n = 371, 70%) and validation group (n = 159, 30%). All patients underwent prostate bpMRI examination, and T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI) sequences were collected before biopsy and were scored, which were respectively named T2WI score and DWI score according to Prostate Imaging Reporting and Data System version 2 (PI-RADS v.2) scoring protocol, and then PI-RADS scoring was performed. We defined a new bpMRI-based parameter named Total score (Total score = T2WI score + DWI score). PI-RADS score and Total score were separately included in the multivariate analysis of the training group to determine independent predictors for csPCa and establish prediction models. Then, prediction models and clinical indicators were compared by analyzing the area under the curve (AUC) and decision curves. A Nomogram for predicting csPCa was established using data from the training group.

Results

In the training group, 160 (43.1%) patients had prostate cancer (PCa), including 128 (34.5%) with csPCa. Multivariate regression analysis showed that the PI-RADS score, Total score, f/tPSA, and PSA density (PSAD) were independent predictors of csPCa. The prediction model that was defined by Total score, f/tPSA, and PSAD had the highest discriminatory power of csPCa (AUC = 0.931), and the diagnostic sensitivity and specificity were 85.1% and 87.5%, respectively. Decision curve analysis (DCA) showed that the prediction model achieved an optimal overall net benefit in both the training group and the validation group. In addition, the Nomogram predicted csPCa revealed good estimation when compared with clinical indicators.

Conclusion

The prediction model and Nomogram based on bpMRI and clinical indicators exhibit a satisfactory predictive value and improved risk stratification for csPCa, which could be used for clinical biopsy decision-making.
Prioritizing a Research Agenda of Transitional Care Interventions for Childhood-Onset Disabilities
Authors: Duncan Andrea , Luong Dorothy , Perrier Laure , Bayley Mark T. , Andrew Gail , Arbour-Nicitopoulos Kelly , Chan Brian , Curran C. J. , Dimitropoulos Gina , Hartman Laura , Huang Lennox , Kastner Monika , Kingsnorth Shauna , McCormick Anna , Nelson Michelle , Nicholas David , Penner Melanie , Thompson Laura , Toulany Alene , Woo Amanda , Zee Joanne , Munce Sarah E. P.
Published: September 2021

Transitional care interventions have the potential to optimize continuity of care, improve health outcomes and enhance quality of life for adolescents and young adults living with chronic childhood-onset disabilities, including neurodevelopmental disorders, as they transition to adult health and social care services. The paucity of research in this area poses challenges in identifying and implementing interventions for research, evaluation and implementation. The purpose of this project was to advance this research agenda by identifying the transitional care interventions from the scientific literature and prioritize interventions for study. A modified-Delphi approach involving two rounds of online surveys followed by a face-to-face consensus meeting with knowledge users, researchers and clinician experts in transitional care (n = 19) was used. A subsequent virtual meeting concluded the formulation of next steps. Experts rated 16 categories of interventions, derived from a systematic review, on importance, impact, and feasibility. Seven of the 16 interventions categories received a mean score rating of ≥7 (out of 10) on all three rating categories. Participants then rank ordered the reduced list of seven interventions in order of priority and the top four ranked interventions advanced for further discussion at a consensus meeting. Using the Template for Intervention Description and Replication (TIDieR) checklist as a guide, the participants identified that a study of apeer system navigatorwas worthy of future evaluation. This study highlighted that transitional care interventions are complex and multifaceted. However, the presence of a peer to support system navigation, advocacy and individual and family education was considered the most ideal intervention addressing the current gap in care. Future research, which aims to engage patients and families in a co-design approach, is recommended to further develop this intervention.
Maternal and Newborn Thyroid Hormone, and the Association With Polychlorinated Biphenyls (PCBs) Burden: The EHF (Environmental Health Fund) Birth Cohort
Authors: Berlin Maya , Barchel Dana , Brik Anna , Kohn Elkana , Livne Ayelet , Keidar Rimona , Tovbin Josef , Betser Moshe , Moskovich Miki , Mandel Dror , Lubetzky Ronit , Ovental Amit , Factor-Litvak Pam , Britzi Malka , Ziv-Baran Tomer , Koren Ronit , Klieger Chagit , Berkovitch Matitiahu , Matok Ilan , Marom Ronella
Published: September 2021

Background:Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants found in human tissues. PCBs can be transferred through the placenta and may disrupt the maternal thyroid homeostasis, and affect fetal thyroid hormone production. Several studies have shown that intrauterine exposure to PCBs might be associated with abnormal levels of thyroid hormones in mothers and their offspring.

Objectives:To examine the associations between environmental exposure to PCBs and thyroid hormone levels in mothers and newborns.

Methods:The EHF-Assaf-Harofeh-Ichilov cohort includes 263 mothers-newborns dyads. A total of 157 mother-newborn dyads had both PCBs and thyroid function measures. Regression models were used to estimate associations between maternal PCB exposure and maternal and newborn thyroid function, controlling for possible confounders.

Results:Four PCBs congeners were analyzed: PCBs 118, 138, 153, and 180. ∑PCBs median (IQR) level was 14.65 (2.83–68.14) ng/g lipids. The median maternal thyroid-stimulating hormone (TSH) level was 2.66 (0.70–8.23) μIU/ml, the median maternal free thyroxine (FT4) level was 12.44 (11.27–13.53) μg/dL, the median maternal thyroid peroxidase antibodies (TPO Ab) level was 9.6 (7.36–12.51) IU/mL. Newborns' median total thyroxine (T4) level was 14.8 (7.6–24.9) μg/dL. No association was found between exposure to different congeners or to ∑PCBs and maternal TSH, FT4, thyroglobulin autoantibodies (Tg Ab), TPO Ab and newborn total T4 levels. In multivariable analysis a 1% change in ∑PCBs level was significantly associated with a 0.57% change in maternal TSH levels in women with body mass index (BMI) < 19. The same association was observed for each of the studied PCB congeners. Maternal TPO Ab levels statistically significantly increased by 0.53 and 0.46% for 1% increase in PCB 118 and 153 congeners, respectively. In women with BMI > 25, the association between the PCBs levels and maternal TSH levels was in the opposite direction. No association was found in women with normal BMI (19–24.9).

Conclusions:Background exposure to environmentally relevant concentrations of some PCBs can alter thyroid hormone homeostasis in pregnant women and might be associated with abnormal TSH levels and TPO-Ab in women with low BMI. However, these findings require further investigation.
Nonlinear Ion-Acoustic Waves in Degenerate Plasma with Landau Quantized Trapped Electrons
Authors: Jahangir R. , Ali S.
Published: September 2021

The formation of nonlinear ion-acoustic waves is studied in a degenerate magnetoplasma accounting for quantized and trapped electrons. Relying on the reductive perturbation technique, a three-dimensional Zakharov–Kuznetsov (ZK) equation is derived, admitting a solitary wave solution with modified amplitude and width parameters. The stability of the ZK equation is also discussed using the k-expansion method. Subsequently, numerical analyses are carried out for plasma parameters of a dense stellar system involving white dwarf stars. It has been observed that the quantized magnetic field parameter η and degeneracy of electrons (determined by small temperature values T) affect the amplitude and width of the electric potential. The critical point at which the nature of the solitary structure changes from compressive to rarefaction is evaluated. Importantly, the growth rate of the instability associated with a three-dimensional ZK equation depends on the plasma parameters, and higher values of η and T tend to stabilize the solitons in quantized degenerate plasmas. The results of the present study may hold significance to comprehend the properties of wave propagation and instability growth in stellar and laboratory dense plasmas.