bluuebunny/crossref_metadata_2025 huggingface dataset:
Applying ICOMOS-IFLA Principles for the Conservation, Management and Reuse of a Historical Hydraulic System: The No-Ras Qanat in North-Western Iran
Authors: Zaina Federico , Branduini Paola , Zavvari Fereshteh
Published: October 2022

Historical hydraulic systems represent a significant part of landscapes and global heritage. However, from the second half of the 20th century onwards, substantial socio-economic as well as technological changes occurring worldwide have put them at risk of abandonment and, eventually, of disappearing. Recent studies and international conventions, including the ICOMOS-IFLA, framed historical hydraulic systems and water management techniques in a new dimension, not only as an element of the past to be preserved but an active element to achieve sustainable economic development and mitigate climate change. Those qanats or karez represented a major historical hydraulic sustainable solution for irrigation, providing a water supply, which during the last few decades, has been slowly replaced with modern, although polluting and unsustainable, technologies. Building on the recent ICOMOS-IFLA Principles Concerning Rural Landscape as Heritage and the recommendation provided by initial research, this paper aims to show how qanats can become: (1) an important local and regional cultural and natural heritage; (2) a valuable economic resource; (3) an environmentally friendly system that could at least partially replace the existing polluting solution (i.e., dams and other modern infrastructures). To achieve these goals, we propose a restoration or reuse approach for the qanat based on the necessity of multiple stakeholders at local and national levels using sustainable materials and respecting the different values as a heritage place. Our case study is the No-Ras qanat in North-western Iran. In the conclusion, we also illustrate the relevance of the aims and methods of this paper in the light of the United Nations Sustainable Development Goals.
Artistic Transfers from Islamic to Christian Art: A Study with Geographic Information Systems (GIS)
Authors: Marcos Cobaleda María
Published: October 2022

The aim of this article is to present the main aspects of the methodology employed in my research concerning artistic transfers in the late medieval Mediterranean from Islamic to Christian art, with a special focus on the Iberian Peninsula. The starting point of the research was the selection of certain artistic elements incorporated into western Islamic art during the Almoravid period (in particular, the muqarnaṣ and the pointed-horseshoe arches), to analyse their spread in western Islamic art and beyond. A Geographic Information System (GIS) was applied to create two databases and assess the distribution of these elements in the Mediterranean framework between the 12th and 15th centuries. As a result, different analyses and cartographic material developed with the GIS are thus included in this work. The GIS made it possible to analyse not only geographic aspects of the distribution of these elements but also other complex phenomena related to the muqarnaṣ and the pointed-horseshoe arches in a quantitative way, which allowed me to raise some preliminary hypotheses concerning the use and distribution of both elements in the Mediterranean framework.
Research on the Use of Thermal Imaging as a Method for Detecting Fungal Growth in Apples
Authors: Lipińska Edyta , Pobiega Katarzyna , Piwowarek Kamil , Błażejak Stanisław
Published: October 2022

This study assesses whether thermal imaging can be utilized for detecting and monitoring the level of plant product contamination with apples used as an example. The growth of Penicillium expansum, Botrytis cinerea, and Rhizopus stolonifer on apples contaminated with these fungi was investigated by measuring temperature changes using a thermovision camera. The results showed a significant relationship between the temperature of apples and the growth of microorganisms, as well as that the temperature changes occurred in two stages (temperature of the contaminated apples increased on the first day but then decreased). Significant differences were found between the temperature of the apples showing microbial growth and the noncontaminated control sample, which indicates that the thermal imaging technique has a potential application in microbial quality control. Automation of the production process and attempts on the intensification of production capacity have resulted in the need to improve individual stages of product quality control. Thermovision-based methods have a high potential in this field, as they represent an innovative and noninvasive alternative to conventional microbiological diagnostic methods.
Development of a Machine Learning Framework to Aid Climate Model Assessment and Improvement: Case Study of Surface Soil Moisture
Authors: Ramírez Casas Francisco Andree , Sushama Laxmi , Teufel Bernardo
Published: October 2022

The development of a computationally efficient machine learning-based framework to understand the underlying causes for biases in climate model simulated fields is presented in this study. The framework consists of a two-step approach, with the first step involving the development of a Random Forest (RF) model, trained on observed data of the climate variable of interest and related predictors. The second step involves emulations of the climate variable of interest with the RF model developed in step one by replacing the observed predictors with those from the climate model one at a time. The assumption is that comparing these emulations with that of a reference emulation driven by all observed predictors can shed light on the contribution of respective predictor biases to the biases in the climate model simulation. The proposed framework is used to understand the biases in the Global Environmental Multiscale (GEM) model simulated surface soil moisture (SSM) for the April–September period, over a domain covering part of north-east Canada. The grid cell-based RF model, trained on daily SSM and related climate predictors (water availability, 2 m temperature, relative humidity, snowmelt, maximum snow water equivalent) from the fifth generation European Centre for Medium-Range Weather Forecasts reanalysis (ERA5), demonstrates great skill in emulating SSM, with root mean square error of 0.036. Comparison of the five RF emulations based on GEM predictors with that based on ERA5 predictors suggests that the biases in the mean April–September SSM can be attributed mainly to biases in three predictors: water availability, 2 m temperature and relative humidity. The regions where these predictors contribute to biases in SSM are mostly collocated with the regions where they are shown to be the among the top three influential predictors through the predictor importance analysis, i.e., 2 m temperature in the southern part of the domain, relative humidity in the northern part of the domain and water availability over rest of the domain. The framework, without having to undertake expensive simulations with the climate model, thus successfully identifies the main causes for SSM biases, albeit with slightly reduced skill for heavily perturbed simulations. Furthermore, identification of the causes for biases, by informing targeted climate model improvements, can lead to additional reductions in computational costs.
Impacts of COVID-19 Prevention and Control Measures on Asthma-Related Hospital and Outpatient Visits in Yichang, China
Authors: Wang Jinyi , Yin Dapeng , Li Guoxing , Wang Tianqi , Zhang Yanli , Gan Hui , Sun Jinfang
Published: October 2022

This article investigates the impact of COVID-19 lockdown and regular epidemic prevention and control after lifting lockdown on asthma-related hospital and outpatient visits in Yichang. Data on the general outpatient department (GOPD), emergency department (ED) and intensive care unit (ICU) visits for asthma from 15 November 2019 to 21 May 2020 and the corresponding from 2018 to 2019 were collected from eight tertiary hospitals in municipal districts. The controlled interrupted time series (CITS) analysis was used to investigate the level and long-term trend changes of weekly asthma visits during lockdown and regular epidemic prevention and control, and stratified by type of visits and age. A total of 9347 asthma-related hospital and outpatient visits were analyzed. The CITS showed that after the implementation of lockdown, the weekly visits of asthma patients immediately decreased by 127.32 (p = 0.002), and the level of GOPD and ED/ICU visits immediately decreased significantly. After implementation of regular prevention, the level and trend of overall weekly visits changed insignificantly compared with the lockdown period. The weekly visits of GOPD adults immediately increased by 51.46 (p < 0.001), and the trend of ED/ICU adults decreased by 5.06 (p = 0.003) visits per week compared with lockdown period. The COVID-19 lockdown in Yichang was related to the decrease in hospital and outpatient visits for asthma. After the implementation of subsequent regular prevention and control measure, only the GOPD visits of adults increased compared with lockdown period.
A Forest Bathing Intervention in Adults with Intellectual Disabilities: A Feasibility Study Protocol
Authors: Bermejo-Martins Elena , Pueyo-Garrigues María , Casas María , Bermejo-Orduna Raúl , Villarroya Ana
Published: October 2022

Forest bathing practices benefit individuals’ physical and mental health. A growing number of published studies provide evidence of such effects in diverse populations and contexts. However, no literature has been found that evaluates the effects of forest bathing on people with intellectual disabilities. In this paper, we present a quasi-experimental pre–post protocol for assessing the preliminary efficacy and feasibility of a forest bathing intervention in a group of adults with intellectual disability. An 11-weekly session program will be applied in the forests of the Ollo Valley, Navarre (Spain). The preliminary efficacy outcomes will be blood pressure, psycho-physiological coherence parameters and quality of life. The feasibility of the intervention will be assessed through data on barriers and facilitators of the implementation process and indicators of environmental comfort (physiological equivalent temperature and thermic perception). This study offers an opportunity for people with intellectual disabilities to benefit from a forest bathing intervention and explore its effects not only on their quality of life, but also on the improvement in their physiological and psychological state. This feasibility study is an essential step to explore crucial aspects for a future full-scale trial.
Experimental Investigation of the Effect of Groundwater on the Relative Permeability of Coal Bodies around Gas Extraction Boreholes
Authors: Pang Mingkun , Pan Hongyu , Zhang Hang , Zhang Tianjun
Published: October 2022

Water infiltration in boreholes is a common problem in mine gas pre-extraction, where water infiltration can significantly reduce the efficiency of gas extraction and curtail the life cycle of the borehole. It is important to evaluate the effect of groundwater on the permeability of the coal body around a gas extraction borehole. In order to determine the seepage parameters of the fractured coal body system around the borehole, a water–gas two-phase seepage test was designed to determine the relative seepage parameters of the fractured coal media seepage system. The main conclusion is that the relative permeability of gas can be effectively increased by increasing the negative extraction pressure at the early stage of extraction to accelerate drainage to reduce the water saturation of the coal seam. Under the combined effect of porosity and seepage pressure, the relative permeability of gas and water in the fractured coal rock body shows three stages. The dependence of the total permeability on the effective stress is closely related to the stages in the evolution of the pore structure, and the total effective permeability decreases with the increase in the effective stress. A decrease in porosity can lead to a decrease in permeability and an increase in the non-Darcy factor. Through an in-depth analysis of the damage and permeability pattern of the coal body around the perimeter of the dipping borehole, the efficient and safe extraction of gas from dipping boreholes in water-rich mines is thus ensured.
Caregiver Burden Is Reduced by Social Support Services for Non-Dependent Elderly Persons: Pre-Post Study of 569 Caregivers
Authors: Arlotto Sylvie , Gentile Stéphanie , Blin Alice , Durand Anne-Claire , Bonin-Guillaume Sylvie
Published: October 2022

Background: Informal care provided by family caregivers (FCGs) to elderly persons is associated with a high risk of burden and poor health status. Social support services (3S) for the elderly persons were characterized by assistance in various activities of daily living. This study aimed to analyze the impact of 3S on the burden of FCGs of elderly persons living in the community and identify factors associated with changes in their burden. Methods: This pre-post study was performed in the southeast of France: FCGs of non-dependent elderly persons still living at home who received a 3S were consecutively included. FCG burden was assessed with the Mini-Zarit scale before the setting up of the 3S (pre-3S) and 6 months after (post-3S). Results: A total of 569 FCGs were included in the study. Mean age of the FCGs was 62.9 years old (±13.3), 67% were women, 61.2% were children or stepchildren. Burden was present for 81% of FCGs. In most cases, 3S targeted household chores (95.8%); 59.8% of elderly persons and their FCGs were fully satisfied. The improvement in burden was greater for FCGs perceiving less obstacles post-3S in helping elderly persons (OR = 4.083) but also for FCGs fully satisfied with the 3S (OR = 2.809) and for FCGs whose perceived health status had improved post-3S (OR = 2.090). Conclusions: FCGs of non-dependent elderly persons experience a burden similar to those of dependent elderly persons. The implementation of a 3S in daily life helps to reduce their burden.
The Influence of a Fire at an Illegal Landfill in Southern Poland on the Formation of Toxic Compounds and Their Impact on the Natural Environment
Authors: Rykała Wojciech , Fabiańska Monika J. , Dąbrowska Dominika
Published: October 2022

Landfill fires pose a real threat to the environment as they cause the migration of pollutants to the atmosphere and water sources. A greater risk is observed in the case of wild landfills, which do not have adequate isolation from the ground. The aim of this article is to present the results of studies on the toxicity of waste from a fire in a landfill in Trzebinia (southern Poland). Both soil and waste samples were investigated. The samples were analyzed using the GC-MS method and the leachates using ICP-OES. A total of 32 samples of incinerated waste and soil were collected. The organic compounds included naphthalene, fluorene, phenanthrene, anthracene, acenaphthene, acenaphthylene, fluoranthene, pyrene, benzo (c) phenanthrene, benzo (a) anthracene, chrysene, benzo (ghi) fluoranthene, benzo (b + k) fluoranthene, benzo (a) fluoranthene, benzo (c) fluoranthene, benzo (a) pyrene, benzo (e) pyrene, perylene, indeno[1,2,3-cd] pyrene, benzo (ghi) perylene, and dibenzo (a + h) anthracene. Among the inorganic parameters, sulfates, chlorides, arsenic, boron, cadmium, copper, lead, and zinc were taken into account. Phenanthrene reached values exceeding 33 mg/L. Fluoranthene dominated in most of the samples. Sulfates and chlorides were present in the samples in concentrations exceeding 400 and 50 mg/L, respectively. Compounds contained in burnt waste may have a negative impact on soil and water health safety. Therefore, it is important to conduct research and counteract the negative effects of waste fires.
Association between Working Time and Burnout Syndrome in Peruvian Military during the Second Epidemic Wave of COVID-19
Authors: Valladares-Garrido Mario J. , Zapata-Castro Luis Eduardo , Picón-Reategui Cinthia Karina , Mesta-Pintado Ana Paula , Picón-Reategui Ronald Alberto , Huaman-Garcia Mariana , Pereira-Victorio César Johan , Valladares-Garrido Danai , Failoc-Rojas Virgilio E.
Published: October 2022

There is scant evidence on the impact of the COVID-19 pandemic on burnout in front-line military personnel and how working time may influence on this condition. We aimed to determine the association between working time and Burnout syndrome in military personnel. A cross-sectional study was conducted using secondary data among 576 military personnel from Lambayeque, Peru during the second wave of COVID-19 in 2021. We used the Maslach Burnout Inventory instrument to measure Burnout Syndrome. We evaluated its association with work time, measured as the number of months that the military member worked during the pandemic. The prevalence of burnout syndrome was 9%. Of the total sample, 39.1% and 10.3% presented depersonalization and emotional exhaustion, respectively. Military personnel working for more than 18 months had a 104% higher prevalence of Burnout syndrome (PR: 2.04, 95%CI: 1.02–4.10). Exposure to a prolonged work time during the pandemic increased the prevalence of Burnout syndrome in military personnel. This information helps to understand the potential effects of the pandemic on this population and provides insight into the time the military members would need rest to prevent Burnout syndrome.
Knowledge and Factors Associated with Breast Cancer Self-Screening Intention among Saudi Female College Students: Utilization of the Health Belief Model
Authors: Shubayr Nasser , Khmees Rola , Alyami Ali , Majrashi Naif , Alomairy Nada , Abdelwahab Siddig
Published: October 2022

Breast cancer (BC) screening is vital, as it is linked to a greater likelihood of survival, more effective treatment, and better quality of life. One of the most extensively applied models for conceptualizing probable barriers and facilitators to the adoption of desired health behavior is the health belief model (HBM). This study aimed to assess the impact of health perception and knowledge on breast self-examination intention (BSE) using HBM. HBM measures specific factors (perceived susceptibility, severity, barriers, benefits, cues to action, and self-efficacy) that impact one’s intention to use BSE. Data were collected from female undergraduate students (n = 680) using a cross-sectional design, stratified simple random sampling, and a self-administered structured online questionnaire. We used multivariable logistic regression to evaluate our assumptions for students who intended to self-examine for BC. For demographic factors, we modified the multivariate model. Most respondents (93%) were under the age of 24 years. Female students from health colleges (48.5%), in their fourth academic year (42.6%), and from the southern region (57.6%) accounted for the majority of the sample. Respondents with a family history of BC were 9.7% of the total. Categories for age, college, region, residency, and BSE intention showed significant differences in their average knowledge scores. The survey revealed that 72.4% were aware of abnormal breast changes. Three constructs of the health belief model (perceived benefit, perceived barriers, and self-efficacy) are good predictors of BSE intention. Theory-based behavioral change interventions are urgently required for students to improve their prevention practices. Furthermore, these interventions will be effective if they are designed to remove barriers to BSE intention, improve female students’ self-efficacy, and enlighten them on the benefits of self-examination.
‘My Work Matters’: A Qualitative Exploration of Why Staff Love Working in Acute Mental Health
Authors: Mair Rachel , Every-Palmer Susanna , Mathieson Fiona , Jenkin Gabrielle
Published: October 2022

Research findings and media coverage of staff experiences of working in mental health settings tend to focus on the negative aspects of the work such as burnout and stress. These negative aspects affect job satisfaction. Job satisfaction can be understood through the lense of Self-Determination theory, which emphasises the importance of autonomy, competence and relatedness (connection) in job satisfaction. This article reports on staff views on positive aspects of working in acute mental health care, drawing on qualitative interview data collected for a larger study of the social and architectural environment of mental health inpatient facilities in New Zealand. Forty-two inpatient mental health staff participated in semi-structured interviews about their experiences of working in such facilities, sharing the positive aspects of working in this setting, including ‘what they liked most’. Responses were thematically analysed using the Framework Method to identify and organise key themes that were refined iteratively, checking for agreement between researchers. Four key themes were identified: work that matters; the people; the physical and social environment and the extrinsic rewards/personal benefits. The results provide an alternative framing of working in acute mental health settings compared, with commonly reported research findings and media coverage focusing on staff burnout and stress in these settings. Despite the much-documented challenges of working in this often poorly resourced and stigmatized area of health, most participants spoke warmly and enthusiastically about what they did, with frequent use of the word ‘love’ in relation to their work. This was largely because they found the work and social relationships rewarding and they were able to make an important contribution to the wellbeing of mental health service users.
How Lifestyle Changes during the COVID-19 Global Pandemic Affected the Pattern and Symptoms of the Menstrual Cycle
Authors: Bruinvels Georgie , Blagrove Richard C. , Goldsmith Esther , Shaw Laurence , Martin Daniel , Piasecki Jessica
Published: October 2022

This research investigated the implications that the COVID-19 pandemic had on the menstrual cycle and any contributing factors to these changes. A questionnaire was completed by 559 eumenorrheic participants, capturing detail on menstrual cycle symptoms and characteristics prior to and during the COVID-19 pandemic lockdown period. Over half of all participants reported to have experienced lack of motivation (61.5%), focus (54.7%) and concentration (57.8%). 52.8% of participants reported an increase in cycle length. Specifically, there was an increase in the median cycle length reported of 5 days (minimum 2 days, maximum 32 days), with a median decrease of 3 days (minimum 2 days and maximum 17 days). A lack of focus was significantly associated with a change in menstrual cycle length (p = 0.038) reported to have increased by 61% of participants. Changes to eating patterns of white meat (increase p = 0.035, decrease p = 0.003) and processed meat (increase p = 0.002 and decrease p = 0.001) were significantly associated with a change in menstrual cycle length. It is important that females and practitioners become aware of implications of environmental stressors and the possible long-term effects on fertility. Future research should continue to investigate any long-lasting changes in symptoms, as well as providing education and support for females undergoing any life stressors that may implicate their menstrual cycle and/or symptoms.
Molecular Design, Spectroscopic, DFT, Pharmacological, and Molecular Docking Studies of Novel Ruthenium(III)–Schiff Base Complex: An Inhibitor of Progression in HepG2 Cells
Authors: Noureldeen Amani F. H. , Aziz Safa W. , Shouman Samia A. , Mohamed Magdy M. , Attia Yasmin M. , Ramadan Ramadan M. , Elhady Mostafa M.
Published: October 2022

A novel ruthenium(III)–pyrimidine Schiff base was synthesized and characterized using different analytical and spectroscopic techniques. Molecular geometries of the ligand and ruthenium complex were investigated using the DFT-B3LYP level of theory. The quantum global reactivity descriptors were also calculated. Various biological and molecular docking studies of the complex are reported to explore its potential application as a therapeutic drug. Cytotoxicity of the complex was screened against cancer colorectal (HCT116), breast (MCF-7 and T47D), and hepatocellular (HepG2) cell lines as well as a human normal cell line (HSF). The complex effectively inhibited the tested cancer cells with variable degree with higher activity towards HepG2 (IC50 values were 29 μM for HepG2, 38.5 μM for T47D, 39.7 μM for HCT, and 46.7 μM for MCF-7 cells). The complex induced apoptosis and cell cycle arrest in the S phase of HepG2 cells. The complex significantly induced the expression of H2AX and caspase 3 and caspase 7 gene and the protein level of caspase 3, as well as inhibited VEGF-A and mTOR/AKT, SND1, and NF-kB gene expression. The molecular docking studies supported the increased total apoptosis of treated HepG2 cells due to strong interaction of the complex with DNA. Additionally, the possible binding interaction of the complex with caspase 3 could be responsible for the elevated activity of caspase 3–treated cells. The score values for the two receptors were −3.25 and −3.91 kcal/mol.
The Effect of 12-Weeks Recreational Football (Soccer) for Health Intervention on Functional Movement in Older Adults
Authors: Duncan Michael J. , Mowle Sophie , Noon Mark , Eyre Emma , Clarke Neil D. , Hill Mathew , Tallis Jason , Julin Mikko
Published: October 2022

There is growing evidence that recreational football offers health benefits for older adults and an important pathway for physical activity for older adult groups. Despite anecdotal evidence that recreational football is beneficial for older adults, no empirical data are available to support this assertion. This study addressed this issue and examined the effects of a 12-week recreational football intervention on the functional fitness of older adults. Using a pre–post case–control design, thirteen males, aged 61–73 years (mean age ± SD = 66 ± 4 years) undertook a twice-weekly, 12-week recreational football for health intervention, and were matched with a control group, comprising thirteen males, aged 62–78 years (mean age ± SD = 66 ± 4 years) who maintained their typical exercise habits during the intervention period. Pre- and postintervention, participants underwent assessment of functional fitness, using the Rikli and Jones functional fitness battery as well as an assessment of body fatness, via bioelectrical impedance analysis and dominant handgrip strength using handgrip dynamometry. Results from a series of 2 (pre–post) X 2 (intervention vs. control) repeated-measures ANOVAs indicate significant pre–post X group interactions for the 30-second chair stand (p = 0.038, Pƞ2 = 0.168), 8-foot timed up and go (p = 0.001, Pƞ2 = 0.577) and 6 min walk test (p = 0.036, Pƞ2 = 0.171). In all cases, performance improved significantly after the intervention for the football intervention group but not the control group. There were no significant differences in the 30 s arm curl test or dominant handgrip strength (p > 0.05). There was a non-significant trend (p = 0.07, Pƞ2 = 0.127) towards a pre–post X group interaction for body fatness, showing a decreased percent body fat for the intervention group over the control group. The results of the present study demonstrate the utility of recreational football as a physical activity intervention in older adults to improve functional movement.
The Influence of Crops on the Content of Polycyclic Aromatic Hydrocarbons in Soil Fertilized with Manure and Mineral Fertilizers
Authors: Mackiewicz-Walec Ewa , Krzebietke Sławomir Józef , Sienkiewicz Stanisław
Published: October 2022

Polycyclic aromatic hydrocarbons (PAHs) are mainly accumulated in soil. Plants secrete enzymes that transform or biodegrade PAHs in soil. Some plant species are more effective in stimulating the biodegradation of these pollutants than other species. This study was undertaken to evaluate the influence of crop rotation on PAH concentrations in soil. Four crops were grown in rotation: sugar beets, spring barley, maize, and spring wheat. Soil samples for the study were obtained from a long-term field experiment established in 1986 in Bałcyny, Poland. The concentrations of PAHs were analyzed in soil samples gathered over a period of 12 years (1998–2009). An attempt was made to evaluate the effect of crop rotation (sugar beets, spring barley, maize, and spring wheat) on PAH concentrations in soil. The content of PAHs in soil samples was measured by gas chromatography with flame ionization detection. Data were processed statistically by repeated measures ANOVA. The concentrations of ∑16 PAHs were lowest in soil after sugar beet cultivation, and highest in soil after maize cultivation. It can be concluded that maize was the plant with the greatest adverse effect on the content of heavy PAH in the soil, a completely different effect can be attributed to spring wheat, which has always been shown to reduce the content of heavy PAH in the soil. Weather conditions affected PAHs levels in soil, and PAH content was highest in soil samples collected in a year with the driest growing season. This arrangement suggests a greater influence of weather conditions than of the cultivated plant.
Study on the Evolution Mechanism of Ecosystem Services in Karst Mountainous Areas from the Perspective of Humanities
Authors: Miao Peipei , Zhao Xiaoqing , Pu Junwei , Huang Pei , Shi Xiaoqian , Gu Zexian
Published: October 2022

Anthropogenic activities have altered ecosystem service functions in the karst mountainous areas. The implementation of ecological restoration projects by the government, the behavior, attitude, and willingness of farmers to participate in their implementation, the application of pesticides and fertilizers, in addition to other socio-economic activities, have had a significant impact on the ecosystem services (ESS) of the region. Taking Guangnan County, a typical karst mountainous area in Yunnan Province, as an example, this study analyzes the evolutionary characteristics of six types of ESS and the driving mechanism of the change in ESS from the anthropogenic macro and micro perspective using questionnaire surveys and the multivariate logistic model. The results showed that (1) ecological restoration projects in the past 20 years have promoted an overall ecological transformation in the typical karst mountainous areas of the Yunnan Province (2) from the macro perspective, and the implementation of such ecological projects is beneficial in increasing soil conservation, carbon sequestration, habitat support, and cultural services. The reduction in agricultural population is beneficial in improving habitat support services, and the increase in the annual average tourism income and the tertiary industry is beneficial in increasing cultural services. Among them, the impact of hydraulic engineering on water production and the tertiary industry on cultural services are the most significant, with the change in the human disturbance index having the most substantial impact on soil conservation, carbon sequestration, and habitat support (3) at the micro level. Increasing pesticide and fertilizer application, willingness and use by farmers has a positive impact on food supply and a negative impact on habitat quality. An increase in the number and willingness of farmers participating in restoring farmland to forests and water conservancy projects was observed. This has a positive impact on soil conservation, water production, and carbon sequestration. Among them, the application of chemical fertilizers and pesticides has the most significant impact on food supply and habitat support, and the willingness to implement the projects on restoring farmlands to forests has the most significant impact on carbon sequestration. The willingness to implement terracing has the greatest impact on water production and soil conservation, and aesthetic value has the greatest impact on cultural services.
Dysregulated Hemostasis and Immunothrombosis in Cerebral Cavernous Malformations
Authors: Globisch Maria Ascencion , Onyeogaziri Favour Chinyere , Smith Ross Osborne , Arce Maximiliano , Magnusson Peetra Ulrica
Published: October 2022

Cerebral cavernous malformation (CCM) is a neurovascular disease that affects 0.5% of the general population. For a long time, CCM research focused on genetic mutations, endothelial junctions and proliferation, but recently, transcriptome and proteome studies have revealed that the hemostatic system and neuroinflammation play a crucial role in the development and severity of cavernomas, with some of these publications coming from our group. The aim of this review is to give an overview of the latest molecular insights into the interaction between CCM-deficient endothelial cells with blood components and the neurovascular unit. Specifically, we underscore how endothelial dysfunction can result in dysregulated hemostasis, bleeding, hypoxia and neurological symptoms. We conducted a thorough review of the literature and found a field that is increasingly poised to regard CCM as a hemostatic disease, which may have implications for therapy.
The Structural Interactions of Molecular and Fibrillar Collagen Type I with Fibronectin and Its Role in the Regulation of Mesenchymal Stem Cell Morphology and Functional Activity
Authors: Nashchekina Yuliya , Nikonov Pavel , Prasolov Nikita , Sulatsky Maksim , Chabina Alina , Nashchekin Alexey
Published: October 2022

The observed differences in the structure of native tissue and tissue formed in vitro cause the loss of functional activity of cells cultured in vitro. The lack of fundamental knowledge about the protein mechanism interactions limits the ability to effectively create in vitro native tissue. Collagen is able to spontaneously assemble into fibrils in vitro, but in vivo, other proteins, for example fibronectin, have a noticeable effect on this process. The molecular or fibrillar structure of collagen plays an equally important role. Therefore, we studied the interaction of the molecular and fibrillar structure of collagen with fibronectin. Atomic force and transmission electron microscopy showed that the presence of fibronectin does not affect the native structure and diameter of collagen fibrils. Confocal microscopy demonstrated that the collagen structure affects the cell morphology. Cells are better spread on molecular collagen compared with cells cultured on fibrillar collagen. Fibronectin promotes the formation of a large number of focal contacts, while in combination with collagen of both forms, its effect is leveled. Thus, understanding the mechanisms of the relationship between the protein structure and composition will effectively manage the creation in vitro of a new tissue with native properties.
GPER Agonist G1 Prevents Wnt-Induced JUN Upregulation in HT29 Colorectal Cancer Cells
Authors: Abancens Maria , Harvey Brian J. , McBryan Jean
Published: October 2022

Women consistently show lower incidence and mortality rates for colorectal cancer (CRC) compared to men. Epidemiological evidence supports a pivotal role for estrogen in protecting women against CRC. Estrogen protective effects in CRC have been mainly attributed to the estrogen receptor beta (ERβ) however its expression is lost during CRC progression. The role of the G-protein coupled membrane estrogen receptor (GPER/GPER1/GPR30), which remains expressed after ERβ loss in CRC, is currently under debate. We hypothesise that estrogen can protect against CRC progression via GPER by modulating the Wnt/β-catenin proliferative pathway which is commonly hyperactivated in CRC. We sought evidence of sexual dimorphism within the Wnt/β-catenin pathway by conducting Kaplan–Meier analyses based on gene expression of the Wnt receptor FZD1 (Frizzled 1) in multiple public domain CRC patient data sets. High expression of FZD1 was associated with poor relapse-free survival rates in the male but not the female population. In female-derived HT29 CRC cell lines, we show that β-catenin nuclear translocation was not affected by treatment with the GPER agonist G1. However, G1 prevented the Wnt pathway-induced upregulation of the JUN oncogene. These novel findings indicate a mechanistic role for GPER in protecting against CRC progression by selectively reducing the tumorigenic effects of hyperactive Wnt/β-catenin signalling pathways in CRC.
Rationale for Combining the BCL2 Inhibitor Venetoclax with the PI3K Inhibitor Bimiralisib in the Treatment of IDH2- and FLT3-Mutated Acute Myeloid Leukemia
Authors: Seipel Katja , Brügger Yvo , Mandhair Harpreet , Bacher Ulrike , Pabst Thomas
Published: October 2022

In October 2020, the FDA granted regular approval to venetoclax (ABT-199) in combination with hypomethylating agents for newly-diagnosed acute myeloid leukemia (AML) in adults 75 years or older, or in patients with comorbidities precluding intensive chemotherapy. The treatment response to venetoclax combination treatment, however, may be short-lived, and leukemia relapse is the major cause of treatment failure. Multiple studies have confirmed the upregulation of the anti-apoptotic proteins of the B-cell lymphoma 2 (BCL2) family and the activation of intracellular signaling pathways associated with resistance to venetoclax. To improve treatment outcome, compounds targeting anti-apoptotic proteins and signaling pathways have been evaluated in combination with venetoclax. In this study, the BCL-XL inhibitor A1331852, MCL1-inhibitor S63845, dual PI3K-mTOR inhibitor bimiralisib (PQR309), BMI-1 inhibitor unesbulin (PTC596), MEK-inhibitor trametinib (GSK1120212), and STAT3 inhibitor C-188-9 were assessed as single agents and in combination with venetoclax, for their ability to induce apoptosis and cell death in leukemic cells grown in the absence or presence of bone marrow stroma. Enhanced cytotoxic effects were present in all combination treatments with venetoclax in AML cell lines and AML patient samples. Elevated in vitro efficacies were observed for the combination treatment of venetoclax with A1331852, S63845 and bimiralisib, with differing response markers for each combination. For the venetoclax and bimiralisib combination treatment, responders were enriched for IDH2 and FLT3 mutations, whereas non-responders were associated with PTPN11 mutations. The combination of PI3K/mTOR dual pathway inhibition with bimiralisib and BCL2 inhibition with venetoclax has emerged as a candidate treatment in IDH2- and FLT3-mutated AML.
Activation of Meningeal Afferents Relevant to Trigeminal Headache Pain after Photothrombotic Stroke Lesion: A Pilot Study in Mice
Authors: Krivoshein Georgii , Bakreen Abdulhameed , van den Maagdenberg Arn M. J. M. , Malm Tarja , Giniatullin Rashid , Jolkkonen Jukka
Published: October 2022

Stroke can be followed by immediate severe headaches. As headaches are initiated by the activation of trigeminal meningeal afferents, we assessed changes in the activity of meningeal afferents in mice subjected to cortical photothrombosis. Cortical photothrombosis induced ipsilateral lesions of variable sizes that were associated with contralateral sensorimotor impairment. Nociceptive firing of mechanosensitive Piezo1 channels, activated by the agonist Yoda1, was increased in meningeal afferents in the ischemic hemispheres. These meningeal afferents also had a higher maximal spike frequency at baseline and during activation of the mechanosensitive Piezo1 channel by Yoda1. Moreover, in these meningeal afferents, nociceptive firing was active during the entire induction of transient receptor potential vanilloid 1 (TRPV1) channels by capsaicin. No such activation was observed on the contralateral hemi-skulls of the same group of mice or in control mice. Our data suggest the involvement of mechanosensitive Piezo1 channels capable of maintaining high-frequency spiking activity and of nociceptive TRPV1 channels in trigeminal headache pain responses after experimental ischemic stroke in mice.
Insulin Diminishes Superoxide Increase in Cytosol and Mitochondria of Cultured Cortical Neurons Treated with Toxic Glutamate
Authors: Pinelis Vsevolod , Krasilnikova Irina , Bakaeva Zanda , Surin Alexander , Boyarkin Dmitrii , Fisenko Andrei , Krasilnikova Olga , Pomytkin Igor
Published: October 2022

Glutamate excitotoxicity is involved in the pathogenesis of many disorders, including stroke, traumatic brain injury, and Alzheimer’s disease, for which central insulin resistance is a comorbid condition. Neurotoxicity of glutamate (Glu) is primarily associated with hyperactivation of the ionotropic N-methyl-D-aspartate receptors (NMDARs), causing a sustained increase in intracellular free calcium concentration ([Ca2+]i) and synchronous mitochondrial depolarization and an increase in intracellular superoxide anion radical (O2–•) production. Recently, we found that insulin protects neurons against excitotoxicity by decreasing the delayed calcium deregulation (DCD). However, the role of insulin in O2–• production in excitotoxicity still needs to be clarified. The present study aims to investigate insulin’s effects on glutamate-evoked O2–• generation and DCD using the fluorescent indicators dihydroethidium, MitoSOX Red, and Fura-FF in cortical neurons. We found a linear correlation between [Ca2+]i and [O2–•] in primary cultures of the rat neuron exposed to Glu, with insulin significantly reducing the production of intracellular and mitochondrial O2–• in the primary cultures of the rat neuron. MK 801, an inhibitor of NMDAR-gated Ca2+ influx, completely abrogated the glutamate effects in both the presence and absence of insulin. In experiments in sister cultures, insulin diminished neuronal death and O2 consumption rate (OCR).
Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology
Authors: Isah Musa Dan’azumi , Atika Bibi , Dippel Stefan , Ahmed Hassan M. M. , Wimmer Ernst A.
Published: October 2022

Sperm marking provides a key tool for reproductive biology studies, but it also represents a valuable monitoring tool for genetic pest control strategies such as the sterile insect technique. Sperm-marked lines can be generated by introducing transgenes that mediate the expression of fluorescent proteins during spermatogenesis. The homozygous lines established by transgenesis approaches are going through a genetic bottleneck that can lead to reduced fitness. Transgenic SIT approaches have mostly focused on Dipteran and Lepidopteran pests so far. With this study, we provide sperm-marked lines for the Coleopteran pest model organism, the red flour beetle Tribolium castaneum, based on the β2-tubulin promoter/enhancer driving red (DsRed) or green (EGFP) fluorescence. The obtained lines are reasonably competitive and were thus used for our studies on reproductive biology, confirming the phenomenon of ‘last-male sperm precedence’ and that the spermathecae are deployed for long-term sperm storage, enabling the use of sperm from first mating events even after secondary mating events for a long period of time. The homozygosity and competitiveness of the lines will enable future studies to analyze the controlled process of sperm movement into the long-term storage organ as part of a post-mating cryptic female choice mechanism of this extremely promiscuous species.
Identification of Candidate Genes and Regulatory Competitive Endogenous RNA (ceRNA) Networks Underlying Intramuscular Fat Content in Yorkshire Pigs with Extreme Fat Deposition Phenotypes
Authors: Ding Yueyun , Hou Yinhui , Ling Zijing , Chen Qiong , Xu Tao , Liu Lifei , Yu Na , Ni Wenliang , Ding Xiaoling , Zhang Xiaodong , Zheng Xianrui , Bao Wenbin , Yin Zongjun
Published: October 2022

Intramuscular fat (IMF) content is vital for pork quality, serving an important role in economic performance in pig industry. Non-coding RNAs, with mRNAs, are involved in IMF deposition; however, their functions and regulatory mechanisms in porcine IMF remain elusive. This study assessed the whole transcriptome expression profiles of the Longissimus dorsi muscle of pigs with high (H) and low (L) IMF content to identify genes implicated in porcine IMF adipogenesis and their regulatory functions. Hundreds of differentially expressed RNAs were found to be involved in fatty acid metabolic processes, lipid metabolism, and fat cell differentiation. Furthermore, combing co-differential expression analyses, we constructed competing endogenous RNAs (ceRNA) regulatory networks, showing crosstalk among 30 lncRNAs and 61 mRNAs through 20 miRNAs, five circRNAs and 11 mRNAs through four miRNAs, and potential IMF deposition-related ceRNA subnetworks. Functional lncRNAs and circRNAs (such as MSTRG.12440.1, ENSSSCT00000066779, novel_circ_011355, novel_circ_011355) were found to act as ceRNAs of important lipid metabolism-related mRNAs (LEP, IP6K1, FFAR4, CEBPA, etc.) by sponging functional miRNAs (such as ssc-miR-196a, ssc-miR-200b, ssc-miR10391, miR486-y). These findings provide potential regulators and molecular regulatory networks that can be utilized for research on IMF traits in pigs, which would aid in marker-assisted selection to improve pork quality.
Filamentous Hemagglutinin of Bordetella pertussis Does Not Interact with the β2 Integrin CD11b/CD18
Authors: Golshani Maryam , Rahman Waheed Ur , Osickova Adriana , Holubova Jana , Lora Jinery , Balashova Nataliya , Sebo Peter , Osicka Radim
Published: October 2022

The pertussis agent Bordetella pertussis produces a number of virulence factors, of which the filamentous hemagglutinin (FhaB) plays a role in B. pertussis adhesion to epithelial and phagocytic cells. Moreover, FhaB was recently found to play a crucial role in nasal cavity infection and B. pertussis transmission to new hosts. The 367 kDa FhaB protein translocates through an FhaC pore to the outer bacterial surface and is eventually processed to a ~220 kDa N-terminal FHA fragment by the SphB1 protease. A fraction of the mature FHA then remains associated with bacterial cell surface, while most of FHA is shed into the bacterial environment. Previously reported indirect evidence suggested that FHA, or its precursor FhaB, may bind the β2 integrin CD11b/CD18 of human macrophages. Therefore, we assessed FHA binding to various cells producing or lacking the integrin and show that purified mature FHA does not bind CD11b/CD18. Further results then revealed that the adhesion of B. pertussis to cells does not involve an interaction between the bacterial surface-associated FhaB and/or mature FHA and the β2 integrin CD11b/CD18. In contrast, FHA binding was strongly inhibited at micromolar concentrations of heparin, corroborating that the cell binding of FHA is ruled by the interaction of its heparin-binding domain with sulfated glycosaminoglycans on the cell surface.
Anti-Citrullinated Peptide Antibodies Control Oral Porphyromonas and Aggregatibacter species in Patients with Rheumatoid Arthritis
Authors: Arleevskaya Marina I. , Boulygina Eugenia A. , Larionova Regina , Validov Shamil , Kravtsova Olga , Shagimardanova Elena I. , Velo Lourdes , Hery-Arnaud Geneviève , Carlé Caroline , Renaudineau Yves
Published: October 2022

Oral microbiome changes take place at the initiation of rheumatoid arthritis (RA); however, questions remain regarding the oral microbiome at pre-RA stages in individuals with clinically suspect arthralgia (CSA). Two cross-sectional cohorts were selected including 84 Tatarstan women (15 early-RA as compared to individuals with CSA ranging from CSA = 0 [n = 22], CSA = 1 [n = 19], CSA = 2 [n = 11], and CSA ≥ 3 [n = 17]) and 42 women with established RA (median: 5 years from diagnosis [IQ: 2–11]). Amplicon sequence variants (ASVs) obtained from oral samples (16S rRNA) were analyzed for alpha and beta diversity along with the abundance at the genus level. A decrease in oral Porphyromonas sp. is observed in ACPA-positive individuals, and this predominates in early-RA patients as compared to non-RA individuals irrespective of their CSA score. In the RA-established cohort, Porphyromonas sp. and Aggregatibacter sp. reductions were associated with elevated ACPA levels. In contrast, no associations were reported when considering individual, genetic and clinical RA-associated factors. Oral microbiome changes related to the genera implicated in post-translational citrullination (Porphyromonas sp. and Aggregatibacter sp.) characterized RA patients with elevated ACPA levels, which supports that the role of ACPA in controlling the oral microbiome needs further evaluation.