bluuebunny/crossref_metadata_2025 huggingface dataset:
Discontinuous Bifurcation of a Soft-Impact System
Authors: Fu Shihui , Liu Yuan , Chávez Joseph Páez
Published: August 2020

In this paper, we investigate discontinuous bifurcations of a soft-impact system, which is nonsmooth at the switching boundary consisting of two parts. We find that there are no periodic orbits located only in the impact zone, and when grazing bifurcation on one part of the switching boundary occurs, the tangency point changes may occur for different bifurcation parameters, which is also verified by numerical simulation. In particular, we discover degenerate inner and external corner bifurcations, which can produce chaotic behavior, for example, period-doubling cascades and a degenerate inner corner bifurcation that induce chaotic responses. In this way, our investigation reveals the presence of narrow bands of chaotic motion induced by the afore mentioned dynamical phenomena.
Temporal Forcing Induced Pattern Transitions Near the Turing–Hopf Bifurcation in a Plankton System
Authors: Wang Wen , Liu Shutang , Liu Zhibin , Wang Da
Published: August 2020

We explore the impact of time-periodic forcing on pattern transitions in a plankton system of reaction–diffusion type. Here, we mainly focus on the forced states near the Turing–Hopf bifurcation. A normal form analysis leads to the finding that weak forcing exhibits a destabilizing effect on the dynamics by exciting the transitions from a spatially homogeneous stationary state to a periodic oscillation in time. The results are obtained by studying the amplitude equations derived using weakly nonlinear analysis in the presence of forcing, which enables us to calculate the changes of states. Examples are given to confirm the theoretical results.
Stimulus-specific functional remodeling of the left ventricle in endurance and resistance-trained men
Authors: Dawkins Tony G. , Curry Bryony A. , Drane Aimee L. , Lord Rachel N. , Richards Cory , Brown Megan , Pugh Christopher J. A. , Lodge Freya , Yousef Zaheer , Stembridge Michael , Shave Rob E.
Published: August 2020

Training-specific functional remodeling of the LV in response to different loading conditions has been recently suggested, but not experimentally tested in the same group of individuals. Our data provide novel evidence of a dichotomous, training-specific LV adaptive response to hemodynamic pressure or volume loading.
The effects of percutaneous coronary intervention on mortality in elderly patients with non-ST-segment elevation myocardial infarction undergoing coronary angiography
Authors: Gündoğmuş Pınar D , Ölçü Emrah B , Öz Ahmet , Tanboğa İbrahim H , Orhan Ahmet L
Published: August 2020

Introduction

Although it is recommended that elderly patients with non-ST-segment elevation myocardial infarction (NSTEMI) should undergo an assessment for invasive revascularization, these patients undergo fewer coronary interventions despite the current guidelines. The aim of the study is to evaluate the effectiveness of percutaneous coronary intervention on all-cause mortalities monthly and annually in the population.

Methods

Three hundred and twenty-four patients with NSTEMI aged 65 years or older who underwent coronary angiography and treated with conservative strategy or percutaneous coronary intervention were included in the study. All demographic and clinical characteristics of the patients were recorded and one-month and one-year follow-up results were analysed.

Results

Two hundred eight cases (64.19%) were treated with percutaneous coronary intervention and 116 cases (35.81%) of the participant were treated with conservative methods. The mean age of the participants was 75.41 ± 6.65 years. The treatment strategy was an independent predictor for the mortality of one-year (HR: 1.965). Furthermore, Killip class ≥2 (HR:2.392), Left Ventricular Ejection Fraction (HR:2.637) and renal failure (HR: 3.471) were independent predictors for one-year mortality.

Conclusion

The present study has revealed that percutaneous coronary intervention was effective on one-year mortality in NSTEMI patients over the age of 65. It is considered that percutaneous coronary intervention would decrease mortality in these patients but it should be addressed in larger population studies.
A top 5 list for French general practice
Authors: Hazard Agnès , Fournier Lucie , Rossignol Louise , Pelletier Fleury Nathalie , Hervé Corentin , Pitel Thibaud , Pino Cécile , Saint-Lary Olivier , Hanslik Thomas , Blanchon Thierry , François Mathilde
Published: August 2020

Background

Medical overuse is an issue that has recently gained attention. The “Choosing Wisely” campaign invited each specialty in each country to create its own top five lists of care procedures with a negative benefit-risk balance to promote dialogue between patients and physicians.

This study aims to create such a list for French general practice.

Methods

A panel of general practitioners (GPs) suggested care procedures that they felt ought to be prescribed less. Using the Delphi method, a short list of those suggestions was selected. Systematic literature reviews were performed for each item on the short list. The results were presented to the panel to assist with the final selection of the top five list.

Results

The panel included 40 GPs. The list includes: i/ antibiotics prescription for acute bronchitis, nasopharyngitis, otitis media with effusion, or uncomplicated influenza, ii/ systematic prostate specific antigen testing in men older than 50, iii/ prescription of cholinesterase inhibitors for mild cognitive impairment and for Alzheimer’s disease and memantine for Alzheimer’s disease, iv/ statins prescription in primary prevention of cardio-vascular risk in older patients, and v/ benzodiazepine or benzodiazepine-like agents prescription for generalised anxiety, insomnia, and for all indications in older patients.

Conclusions

This study resulted in a French top five list in general practice using a panel of GPs. All the items selected have a negative risk-benefit balance and are frequently prescribed by French general practitioners. This list differs from other top five lists for general practice, reflecting the local medical culture.
Suicide ideation, attempt, and determinants among medical students Northwest Ethiopia: an institution-based cross-sectional study
Authors: Desalegn Getachew Tesfaw , Wondie Mesele , Dereje Saron , Addisu Adanech
Published: August 2020

Background

Suicide ideation and attempt are more highly prevalent among medical students compared to the general population. Suicidal thought negatively impacts the quality of life, physical, and mental well-being of the students. However, research into suicide ideation and attempt among medical students in low- and middle-income countries is limited. Therefore, this study aimed to explore suicide ideation and attempt and their determinants among medical students in Ethiopia have a crucial role for further intervention.

Methods

An institution-based cross-sectional study was conducted on 393 medical students from March to June 2019 at the University of Gondar (UoG) in Ethiopia. Simple random sampling technique was used to select study participants. Suicide ideation and attempt were assessed using the World Health Organization Composite International Diagnostic Interview (CIDI) to assess lifetime suicide ideation and attempts during medical school. Data were collected using a self-administered questionnaire. Binary logistic regression analyses were used to identify factors associated with suicide ideation and attempt. An odds ratio (OR) with a 95% confidence interval (CI) was computed to assess the strength of the association.

Results

A total of 393 participants took part with a response rate of 92.9%. The prevalence of suicide ideation and attempt among study participants was found to be 14% and 7.4%, respectively, with 95% CI (10.9–18.1) and (5.1–10.2). Being female (AOR: 5.21, 95% CI 3.42–7.20), co-morbid depression symptoms (AOR: 10.12, 95% CI 6.80–15.52), current khat chewing (AOR: 4.46, 95% CI 3.32–10.02), and poor social support (AOR: 4.46, 95% CI 3.43–9.87) were factors significantly associated with suicide ideation; whereas, female sex (AOR: 8.08, 95% CI 6.04–12.39), depression (AOR: 10.66, 95% CI 8.01–19.01)and history of mental illness (AOR: 5.53, 95% CI 5.20–15.50) were factors significantly associated with suicide attempt.

Conclusions

In the current study, the prevalence of suicide ideation among medical students was low compared to other studies, but the suicide attempt was high. Ministry of Health should develop a guideline on how to screen and manage suicide ideation and attempt among medical students.
Species diversity of different insect families trapped under beer-based volatile fermentation
Authors: Iqbal Mazher Farid , Feng Yu-Long
Published: August 2020

Background

Insect species composition is an important phenomenon playing a significant role in the ecosystem. Chemical control of insects and pests releases toxic materials to the environment. These chemicals are dangerous to human populations. In this situation, there is a dire need to develop strategies to overcome the haphazard use of chemicals. The present investigations were carried out to explore the diversity of different insects attracted through bait fermentation.

Methods

The traditionally prepared bait fermentation was used to attract different insect populations both in treated (traps installed near field crops) and control traps (traps installed near invasive weed). Abundance, evenness, richness and equitability of these trapped insects were calculated. The chemical screening of bait fermentation was done using Gas Chromatography and Mass Spectrometry (GC–MS).

Results

Significant difference (P < 0.05) in abundance of insect populations was found in treated compared to control trap. The insects of Noctuidae family recorded high Shannon- Wiener’s diversity index followed by Muscidae. Margalef’s index was recorded maximum in the treated traps (10.77) compared to those of control (8.09). The yielded index indicated that maximum richness was found in bait treated compared to control. The Shannon’s equitability’s values were investigated higher in Noctuidae (1.48), while, maximum evenness was observed in Muscidae (2.05) in treated trap. This fermentation was dried at room temperature and ground at 0.1 micron size. Our result showed significant (P < 0.05) effects of extraction times, with high yield in first extraction by polar solvents. Co-efficient of determination (R2= 0.87) recorded similar results in both extractions, however high root mean square error (0.97) recorded with bait + distilled water solvent showed linear arc line gave better performance. Finally, this fermentation was analyzed using GC–MS and recorded volatile compounds that were involved in the attraction of major and minor pests.

Conclusion

Fermentation can help for the attraction of different families of insects of various crops. The field experiment suggested that this fermentation is economical, easily installed and consumed only 0.64 RMB/0.09 USD, including infrastructures per location. Bait fermentation is safe biochemical constituents and did not spread any toxic chemicals to the environment.
Synthesis, molecular docking and molecular dynamic simulation studies of 2-chloro-5-[(4-chlorophenyl)sulfamoyl]-N-(alkyl/aryl)-4-nitrobenzamide derivatives as antidiabetic agents
Authors: Thakral Samridhi , Narang Rakesh , Kumar Manoj , Singh Vikramjeet
Published: August 2020

A series of 2-chloro-5-[(4-chlorophenyl)sulfamoyl]-N-(alkyl/aryl)-4-nitrobenzamide derivatives(5a–5v)has been synthesized and confirmed by physicochemical(Rf, melting point) and spectral means (IR,1HNMR,13CNMR). The results of in vitro antidiabetic study against α-glucosidase indicated that compound5obearing 2-CH3-5-NO2substituent on phenyl ring was found to be the most active compound against both enzymes. The electron donating (CH3) group and electron withdrawing (NO2) group on a phenyl ring highly favoured the inhibitory activity against these enzymes. The docking simulations study revealed that these synthesized compounds displayed hydrogen bonding, electrostatic and hydrophobic interactions with active site residues. The structure activity relationship studies of these compounds were also corroborated with the help of molecular modeling studies. Molecular dynamic simulations have been done for top most active compound for validating its α-glucosidase and α-amylase inhibitory potential, RMSD analysis of ligand protein complex suggested the stability of top most active compound5oin binding site of target proteins. In silico ADMET results showed that synthesized compounds were found to have negligible toxicity, good solubility and absorption profile as the synthesized compounds fulfilled Lipinski’s rule of 5 and Veber’s rule.
Correction to: Dynamic simulation of continuous mixed sugar fermentation with increasing cell retention time for lactic acid production using Enterococcus mundtii QU 25
Authors: Wang Ying , Chan Ka-Lai , Abdel-Rahman Mohamed Ali , Sonomoto Kenji , Leu Shao-Yuan
Published: August 2020

An amendment to this paper has been published and can be accessed via the original article.
Hilar lymphadenopathy, a novel finding in the setting of coronavirus disease (COVID-19): a case report
Authors: Mughal Mohsin Sheraz , Rehman Rameez , Osman Ramy , Kan Nathan , Mirza Hasan , Eng Margaret H.
Published: August 2020

Background

As the outbreak of coronavirus disease 2019 (COVID-19) has progressed, computed tomography has emerged as an integral part of the diagnosis alongside reverse transcriptase–polymerase chain reaction assays. Frequently encountered imaging findings include peripheral airspace consolidations; bilateral ground-glass opacities; and, less commonly, cavitation. Hilar lymphadenopathy is a rarely reported finding in the setting of COVID-19.

Case presentation

A 73-year-old Caucasian woman presented to our hospital with fever and fatigue. She had a maximum body temperature of 102.3 °F with lymphopenia and thrombocytopenia. She was diagnosed with severe acute respiratory syndrome coronavirus 2 infection on the basis of a positive result from a reverse transcriptase–polymerase chain reaction of a nasopharyngeal swab sample. Contrast-enhanced chest computed tomography revealed multifocal, subpleural ground-glass opacities with nodular consolidations bilaterally. Computed tomography also demonstrated atypical bilateral hilar lymphadenopathy, a rarely reported imaging feature of COVID-19. Chest computed tomography 1 month before the presentation did not show focal consolidations or lymphadenopathy. This indicated that the findings were due to the patient’s severe acute respiratory syndrome coronavirus 2 infection. She received 5 days of oral hydroxychloroquine and experienced resolution of her symptoms.

Conclusion

Chest computed tomography has been used extensively to diagnose and characterize the distinguishing radiological findings associated with viral pneumonia. It has emerged as an integral part of the diagnosis of COVID-19 alongside reverse transcriptase–polymerase chain reaction assays. Clinicians must be aware of uncommon clinical and radiological findings in order to diagnose this entity. Hilar lymphadenopathy is commonly seen with fungal infections, mycobacterial infections, and sarcoidosis. An extensive literature review found that bilateral hilar lymphadenopathy has not been reported in the setting of COVID-19. More data are needed to establish the clinical impact of this novel finding.
Changes of plasma fibronectin and fibronectin-fibrin complexes in dams of stillborn dairy calves
Authors: Jawor Paulina , Krzyżanowska-Gołąb Dorota , Bajzert Joanna , Stefaniak Tadeusz , Kątnik-Prastowska Iwona
Published: August 2020

Background

Fibronectin (FN) is a large (450–500 kDa), multidomain and multifunctional glycoprotein existing in mammalian tissues. Some fibronectin (FN) molecular forms might be involved in biological processes occurring within the perinatal period, such as tissue remodeling, coagulation, and repair.

Results

In this study fibronectin (FN) and fibrinogen (Fb) concentrations and FN-fibrin complexes occurrence and its relative amounts with increasing high molecular masses were respectively determined by ELISA, heat precipitation, and SDS-agarose-immunoblotting methods. Plasma samples from three groups of dams with: 1) singleton stillborn calf without or with negligible autolytic changes in internal organs (DSBn), 2) singleton stillborn calf with advanced autolytic changes in internal organs (DSBa), 3) singleton live-born control calf (DC), and 4) a group of cows during mid to late lactation (LC) were analyzed. Maternal plasma FN concentration in the DSBn and DSBa groups was significantly lower than in the LC group. The plasma samples of DSBa showed a significantly lower FN concentration than in the DC group. Plasma Fb concentration was significantly higher in the DSBa and DSBn, than in the LC group. FN immunoblotting of the cow plasma samples revealed, besides an FN-dimer band, the presence of supramolecular FN-fibrin bands corresponding to FN-fibrin complexes with increasing molecular masses: up to 5 bands from 750 kDa to 1900 kDa in the DSBn and DSBa plasma samples, two bands of 750 and 1000 kDa in the DC group, and only the smallest one of 750 kDa in the LC group.

Conclusions

The observed low FN concentration and occurrence of supramolecular FN-fibrin complexes (1000 kDa and more) in the maternal plasma comparing to cows in lactation might have been associated with periparturient changes in tissues. The presence in maternal plasma of high-molecular FN-fibrin complexes (1300–1900 kDa) arouse the question if this is the consequence of calf perinatal mortality.
Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
Authors: Oizel Kristell , Yang Chendong , Renoult Ophelie , Gautier Fabien , Do Quyen N. , Joalland Noemie , Gao Xiaofei , Ko Bookyung , Vallette François , Ge Woo-Ping , Paris François , DeBerardinis Ralph J. , Pecqueur Claire
Published: August 2020

Background

Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes.

Methods

To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 (13C) after 13C-glutamine infusion.

Results

Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore, both glutamine synthetase and transglutaminase-2 were expressed accordingly to GBM metabolic phenotypes.

Conclusion

Thus, our results outline the specific enhanced glutamine flux in vivo of the aggressive mesenchymal GBM subtype.
Spatial and temporal variations in depositional systems in the Kazusa Group: insights into the origins of deep-water massive sandstones in a Pleistocene forearc basin on the Boso Peninsula, Japan
Authors: Takao Akihiko , Nakamura Keisuke , Takaoka Shinichi , Fuse Masaya , Oda Yohei , Shimano Yasushi , Nishida Naohisa , Ito Makoto
Published: August 2020

A detailed chronostratigraphic framework established by the mapping of tephra key beds and application of oxygen isotopic data allows assessment of the synchroneity and diachroneity of depositional systems formed in coastal and deep-water environments. This framework also allows estimation of the timing of active delivery of coarse-grained sediments beyond the shelf margin in relation to relative sea-level changes. The depositional processes of deep-water massive sandstones (DWMSs) are still enigmatic; their formation is a result of active delivery of sands in association with the supply of organic carbon into deep-water environments. DWMSs are also important as reservoirs for hydrocarbon explorations. This study investigated the origins of DWMSs in the upper Umegase, Kokumoto, and Chonan formations (in ascending order) of the Pleistocene Kazusa Group on the Boso Peninsula, central Japan. Each formation contains several packets of DWMSs that are interpreted to have formed in response to the progradation of gravelly shelf-margin deltas or fan deltas during the falling and lowstand stages of relative sea-level changes controlled primarily by glacioeustasy. The development of DWMSs and associated sandstone beds is interpreted to have been induced by hyperpycnal flows, in association with sediment gravity flows that were initiated by breaching and/or collapse of sandy substrates on the shelf-margin deltas or fan deltas. The timings of the initial and final deposition of the packets vary within and between the formations, and are considered to have been controlled by the interaction between allogenic and autogenic processes operating in the gravelly shelf-margin deltas or fan deltas. A muddy horizon that contains the Lower–Middle Pleistocene Subseries boundary (the base of the Chibanian Stage) in the Kokumoto Formation is also underlain and overlain by the packets and represents a deposit formed in a condensed section in an upper slope environment. This depositional setting may have favored the development of the Global Boundary Stratotype Section and Point (GSSP) for the Lower–Middle Pleistocene Subseries boundary in the formation.
Another way of “amusing ourselves to death”? Experience, ideological fantasy, and labor control in variety entertainment production
Authors: Jia Wenjuan , Zhong Kaiou
Published: August 2020

Compared with traditional industries, new changes have taken place in the labor process and manufacturing consent in cultural and creative industries. In entertainment labor, managers transform high-intensity labor into pleasant entertainment through relational work, emotional work, and sentimental work. In contrast to traditional industries, labor control in entertainment does not rely on organizational institutions or economic incentives but is based on supplemental cultural factors, such as relationships, emotion, and sentiment. At the same time, the logic of ideological operation behind manufacturing the consent of laborers is no longer according to the traditional Marxist-Althusserian interpretation in which managers shape a particular idea through the control of the labor process while concealing the real labor–capital relation with a false consciousness. It becomes a case of a Lacanian–Zizekist description: the laborer enters a realistic fantasy through specific experience and pursues hisplus-de-jouirin ideological reality. In the end, the laborers devote their body and soul to work.
Exploration and identification of Anredera cordifolia morphological characters in the highlands and lowlands
Authors: Hasanah Yaya , Mawarni Lisa
Published: August 2020

Abstract. Hasanah Y, Mawarni L. 2020. Exploration and identification of Anredera cordifolia morphological characters in the highlands and lowlands. Biodiversitas 21: 2759-2766. Information about genetic diversity and relationships between accessions is very important in plant breeding programs, because with the availability of this information, it is easier to determine relationship between accessions that can be used as a basis for plant selection. The objective of the research was to evaluate the morphological characteristics and relationship of binahong or Anredera cordifolia (Ten.) Steenis) accessions in the lowlands and highlands. The study was conducted in Karo (highlands) and Medan (lowlands), North Sumatra, Indonesia, from May to August 2019. This research used a descriptive method. Before the survey and exploration are conducted, first a pre-survey was conducted by gathering information from key informants and other informants. The characteristic of morphological diversity A. cordifolia can be distinguished based on leaf color, stem shape, axillary tuber, rhizome, and the presence of flowers. The dendrogram relationship between accessions is based on a genetic similarity matrix using cluster analysis. Analysis of qualitative morphological characters revealed the existence of variability among A. cordifolia accessions. There is a far relationship relation between A. cordifolia plants in Karo and Medan with Eucludian range of 0.446 to 57.725, hence resulting in two clusters that show differences in the variation of A. cordifolia plants. There is a distant relationship relation with M4 and M7 accessions with a dissimilar distance of 57,725 and the closest relationship with accession K1 and K2 with a dissimilar distance of 0.446. Therefore, morphological characterization in the research is valuable to understand the variability of genetic of A. cordifolia accessions in the lowland and highland in North Sumatra.
Plant diversity and composition at three Imperata grasslands in Bogor, Katingan, and Kupang, Indonesia
Authors: Usmadi Didi , JOKO RIDHO WITONO  , RENI LESTARI  , DIDIK WIDYATMOKO  , MAHAT MAGANDHI  , IYAN ROBIANSYAH  , ARIEF NOOR RACHMADIYANTO  , DANANG WAHYU PURNOMO  , RIZMOON NURUL ZULKARNAEN  , REZA RAMDAN RIVAI  , HENDRA HELMANTO  , ANGGA YUDAPUTRA  , FRISCA DAMAYANTI
Published: August 2020

Abstract. Usmadi D, Witono JR, Lestari R, Widyatmoko D, Magandhi M, Robiansyah I, Rachmadiyanto AN, Purnomo DW, Zulkarnaen RN, Rivai RR, Helmanto H, Yudaputra A, Damayanti F. 2020. Plant diversity and composition at three Imperata grasslands in Bogor, Katingan, and Kupang, Indonesia. Biodiversitas 21: 2804-2813. Imperata grassland is among the largest proportions of marginal lands in Indonesia. Despite the dominance of Imperata cylindrica grass, certain plant species are able to compete and live together with this species, suggesting window of opportunity that the grasslands can be restored. Yet, the existing diversity and composition of plants in Imperata grasslands may vary due to differences in climatic and soil conditions. This study aims to determine the plant diversity and composition at three Imperata grasslands in Bogor, Katingan, and Kupang, Indonesia. Data were collected using systematic sampling by establishing quadratic observation plots with size of each plot was 1 x 1 m2. The total number of species found in Imperata grasslands in those three study sites was 81 species, belonging to 68 genera and 30 families. In the Bogor site, the dominant species were Imperata cylindrica, Ageratum conyzoides, and Phyllanthus niruri, while in the Katingan site was dominated by I. cylindrica, Scleria ciliaris, and Cyanthillium cinereum and in the Kupang site was dominated by I. cylindrica and Tridax procumbens. The Imperata grassland in Bogor had low species richness, moderate species diversity, the abundance distribution of each species tends to be uniform, and the occurred species did not tend to dominate. The Katingan and Kupang Imperata grasslands had low species richness, low species diversity, the abundance distribution of each species tends to be uneven, and were more dominated by I. cylindrica. Controlling abundance of I. cylindrica is required in those three research sites in order to stimulate the growth of other plant species, which is in turn to maintain and restore biodiversity.
The growth of three teak (Tectona grandis) clones and its effect on wood properties
Authors: Putro Gudiwidayanto Sapto , Marsoem Sri Nugroho , Sulistyo Joko , Hardiwinoto Suryo
Published: August 2020

Abstract. Putro GS, Marsoem SN, Sulistyo J, Hardiwinoto S. 2020. The growth of three teak (Tectona grandis) clones and its effect on wood properties. Biodiversitas 21: 2814-2820. Various clones of superior teak (Tectona grandis L. f.) trees have recently been introduced in several land conditions. A study was conducted on three different clones of superior teak known as Jati Unggul Nusantara (JUN) growing in Paliyan, Gunungkidul, Yogyakarta, Indonesia. Two different ages of those clones were observed their growth rate and its wood properties. Strip plot design was applied to the treatment of tree age (five and eight-year-old) and clone types (D14, F35, and F21 clones). Tree growth was measured on 300 trees, meanwhile, annual growth rate and wood properties were measured on 18 trees. The result showed that at five-year-old as well as eight-year-old, the D14 clone has the best growth performance with diameter of 14.6 ± 1.80 cm and 20.5 ± 2.54 cm and height of 9.8 ± 1.72 m and 15.2 ± 2.13 m respectively. The growth rate negatively correlated with temperature and had no significant correlation to the precipitation. Different clones and tree age significantly affect the variability of fiber length, green specific gravity, and heartwood proportion. The clone of D14 produced the highest-fiber length, green specific gravity, and heartwood proportion (1.111mm; 0.50 and17.7%) at five-year-old trees and (1.156 mm; 0.54 and 49.0%) at eight-years-old.
Short Communication: Detection of bioactive compounds in essential oil from lemongrass cultivated in Ngantang, Malang, East Java, Indonesia
Authors: Hartatie Endang Sri , INDAH PRIHARTINI  , WAHYU WIDODO  , AHMAD WAHYUDI
Published: August 2020

Abstract. Hartatie ES, Prihartini I, Widodo W, Wahyudi A. 2020. Short Communication: Detection of bioactive compounds in essential oil from lemongrass cultivated in Ngantang, Malang, East Java, Indonesia. Biodiversitas 21: 2821-2825. Lemongrass (Cymbopogon citratus) commonly known as West Indian lemongrass is a member of the Poaceae family and belongs to genus Cymbopogon. It is one of the plants commonly used in fresh form as a spice by the people in Indonesia and widely planted in the yard of the house. Lemongrass contains several bioactive compounds and it might be extracted to obtain essential oils useful for many applications such as flavor and fragrance ingredients, perfumery, cosmetics, food preservation, pharmaceutical. The chemical composition of essentials oil can be variable, according to the genetic diversity, habitat, weather, and cultural treatment. The aim of study was to investigate the bioactive compounds in essential oil from lemongrass stalk and lemongrass leaves which cultivated in Ngantang, Malang, East Java, Indonesia. Detection of bioactive compounds was carried out by gas chromatography-mass spectrometry (GC-MS). The results of study are the main compound in lemongrass essential oil was citral. There is a difference between the citral content of essential oils from lemongrass stalk compared to essential oils from lemongrass leaves, both in quantity and components. The citral (geranial and neral) content of essential oil made from stalk was higher than made from leaves, but the amount of bioactive compounds of essential oils from leaves more than from the stalk.
Evaluation of physical and chemical characteristics of sixty peanut germplasm
Authors: Erliana Ginting  , NOVITA NUGRAHAENI
Published: August 2020

Abstract. Ginting E, Nugrahaeni N. 2020. Evaluation of physical and chemical characteristics of sixty peanut germplasm. Biodiversitas 21: 2840-2847. Nutritional values of peanut, particularly protein and fat is essential in terms of predominant use of peanut for foods. The seed size is also important for end use of the products. This study aimed to evaluate the physical and chemical characteristics of sixty ILETRI's peanut germplasm was performed. The results showed that the major genotypes had rose seed coat colors with two seeds per pod and average dried pod yield of 3.09 t/ha. Twenty-one genotypes belonged to large-seeded (≥ 55 g/100 seeds) and thirty-six were medium (40-55 g). The ash contents slightly varied from 2.53% to 2.98% (dw). One genotype (MLGA 0444) contained high protein (> 30% dw), while high-fat contents (> 43% dw) were noted in all genotypes. Selected genotypes had favored combinations of seed size, protein, and fat contents with high pod yield. Based on the similarity of 100-seed weights, ash, protein, and fat contents, sixty genotypes were grouped into three clusters with the main differences in seed sizes. Nineteen genotypes in cluster II with large seed size, medium protein, and high-fat content characteristics, particularly are suitable for oil extraction breeding purposes.
Structural variability of Aleppo pine stands in two forests in northeastern Algeria
Authors: Rached Kanouni Malika , Hani Insaf , BOUSBA Ratiba , Beldjazia Amina , KHAMAR Hichem
Published: August 2020

Abstract. Rached-Kanouni M, Hani I, Bousba R, Beldjazia A, Khammar H. 2020. Structural variability of Aleppo pine stands in two forests in northeastern Algeria. Biodiversitas 21: 2848-2853. The layout of the stand can be described as the width of the trees, their reciprocal locations, diametric distinction and height. The goal of this study is to recognize changes in the Pinus halepensis spatial and demographic systems in two Beni Oudjana and Chettaba Forests, located in northeast Algeria. An inventory of trees in these forest formations with P. halepensis dominance was carried out based on dendrometric parameters such as total height, tree diameter at dbh ≥ 5 cm, basal area, total volume, etc., as well as the number of trees in the forest. Tree diameter and height measurements were made on 12 rectangular plots (20 m × 20 m), located in both forests. The results obtained show that the mean stand density, mean diameter, basal area and total volume are higher in Chettaba Forest, the values attributed to these parameters are respectively (422 trees/ha, 27.07 cm, 26, 86 m2, 251.63 m3); while the total height and regeneration rate show significantly higher values in Beni Oudjana Forest (18.97 m, 607 individuals/ha). The structure in diameter and height of the species is bell-shaped to asymmetrically positive with a predominance of small diameter individuals in the Chettaba forest. On the other hand, in the Beni Oudjana Forest, the structure is ‘L’ shaped, showing a predominance of very small diameter individuals. These results indicate that the low regeneration rate of P. halepensis in the Chettaba Forest is due to anthropogenic pressures that favor the degradation of this forest.
Isolation and selection of Bacillus cereus specific phages from hospital wastewater
Authors: Rovik Anwar , SAEFUDDIN ‘AZIZ  , HENDRO PRAMONO
Published: August 2020

Abstract. Rovik A, ‘Aziz S, Pramono H. 2020. Isolation and selection of Bacillus cereus specific phages from hospital wastewater. Biodiversitas 21: 2871-2877. Bacillus cereus (B. cereus) is a pathogenic bacterium that frequently contaminates food by producing entero and emetic toxins. B. cereus has shown resistance to various antibiotics, especially β-lactam antibiotics. An alternative to control B. cereus contamination is the use of bacteriophages. This study aimed to isolate and screen B. cereus specific phages from hospital wastewater in Banyumas District. The research was conducted descriptively through isolation, purification, titer determination, host ranges, and adsorption rate determination. A total of 29 isolates of B. cereus-phages were isolated from hospital wastewater in Banyumas with various titers, ranged from 0.14-3.76 x 107 PFU.mL-1. Isolated phages could be grouped into two, narrow host range (14 phages) and broad activity spectra (15 phages) that infect both Gram-positive and negative bacteria i.e. B. subtilis, B. fragilis, B. licheniformis, Citrobacter freundii, Klebsiella pneumoniae, Salmonella thypi, and Escherichia coli. The infections had a latency period of 60-120 minutes. The decrease of culture absorbance value ranged from 0.06 to 0.41, while the control tended to increase by 0.39. The largest decreases were showed by phage isolates RSBMS-2 and RSBMT-1 with 0.41 and 0.37, respectively.
Spectral observation of agarwood by infrared spectroscopy: The differences of infected and normal Aquilaria microcarpa
Authors: Adi Danang Sudarwoko , SUNG-WOOK HWANG  , DWI AJIAS PRAMASARI  , YUSUP AMIN  , BERNADETA AYU WIDYANINGRUM  , TEGUH DARMAWAN  , ERIS SEPTIANA  , WAHYU DWIANTO  , JUNJI SUGIYAMA
Published: August 2020

Abstract. Adi DS, Hwang SW, Pramasari DA, Amin Y, Widyaningrum BA, Darmawan T, Septiana E, Dwianto W, Sugiyama J. 2020. Spectral observation of agarwood by infrared spectroscopy: The differences of infected and normal Aquilaria microcarpa. Biodiversitas 21: 2893-2899. This study was conducted to evaluate and to determine the potential spectral band assignments that influenced the differentiation of normal and infected agarwood of Aquilaria microcarpa using Fourier Transform Infrared Spectroscopy (FTIR) and Fourier Transform Near-Infrared Spectroscopy (FTNIR). The results showed that the differences in band intensity on FTIR were identified as C=O stretching (lignin), COO-stretching (hemicellulose), aromatic skeletal vibration (lignin), and C-H bending vibration. The increasing absorbances of infected agarwood were supposed as the change on the wood tissues due to the releasing resinous compound. The C-O bond (aromatic alkane) and stretching (ether), C-C stretching (aromatic alkane), and C-H bond (aromatic ring) which related to the scented fragrance of agarwood have appeared on the FTIR spectra. Multivariate analysis with principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) of the second derivative NIR spectra at the wavenumber 8,000-4,000 cm-1 showed that normal and infected agarwood was successful to be separated. Discriminant model one-on-one classification exhibited good performances as the R2 performance (R2P) values was 0.99. There were eight major wood components which contributed to the separation based on NIR spectra, where lignin, hemicellulose, and xylans were the most valuable chemical compound.
Short Communication: Diversity of culturable epiphytic bacteria isolated from seagrass (Halodule uninervis) in Thailand and their preliminary antibacterial activity
Authors: PARIMA BOONTANOM  , Chantarasiri Aiya
Published: August 2020

Abstract. Boontanom P, Chantarasiri A. 2020. Short Communication: Diversity of culturable epiphytic bacteria isolated from seagrass (Halodule uninervis) in Thailand and their preliminary antibacterial activity. Biodiversitas 21: 2907-2913. Epiphytic bacteria are symbiotic bacteria that live on the surface of seagrasses. This study presents the diversity of culturable epiphytic bacteria associated with the Kuicheai seagrass (H. uninervis) collected from Rayong Province in Eastern Thailand. Nine epiphytic isolates were identified into four phylogenetical genera based on their 16S rRNA nucleotide sequence analyses. They are considered firmicutes in the genera of Planomicrobium, Paenibacillus and Bacillus, and proteobacteria in the genus of Oceanimonas. Three species of epiphytic bacteria preliminarily exhibited antibacterial activity against the human pathogenic Staphylococcus aureus using the perpendicular streak method. The knowledge obtained from this study increases understanding the diversity of seagrass-associated bacteria in Thailand and suggests the utilization of these bacteria for further pharmaceutical applications.
Effectiveness of oral irrigation with an extract of green microalga Nannochloropsis oculata as an anti-inflammatory in rats infected with Aggregatibacter actinomycetemcomitans
Authors: SYAMSULINA REVIANTI  , DWI ANDRIANI  , KRISTANTI PARISIHNI  , ENDAH WAHJUNINGSIH  , WIDYASTUTI
Published: August 2020

Abstract. Revianti S, Andriani D, Parisihni K, Wahjuningsih E, Widyastuti. 2020. Effectiveness of oral irrigation with an extract of green microalga Nannochloropsis oculata as an anti-inflammatory in rats infected with Aggregatibacter actinomycetemcomitans. Biodiversitas 21: 2977-2981. Aggressive periodontitis is strongly correlated with Aggregatibacter actinomycetemcomitans, a periodontopathic bacterium, which releases endotoxins and lipopolysaccharides (LPS). LPS acts as a stimulus to a variety of host cells that can promote the expression of pro-inflammatory cytokines, such as the tumor necrosis factor-α (TNF-α), in periodontal disease. The marine green microalga Nannochloropsis oculata has anti-inflammatory properties. This study aimed to examine the anti-inflammatory efficacy of oral irrigation with N. oculata extract against periodontitis induced by A. actinomycetemcomitans in a rat model. Twenty-four male Wistar rats were divided randomly into four groups (n = 6). First control group was without any treatment, the second to fourth groups were infected with A. actinomycetemcomitans and then orally irrigated with N. oculata extract at concentrations of 2.375%, 2.5%, and 2.625%. The mandibles of the euthanized rats were hemisected to measure the expression of TNF-α, interleukin-10 (IL-10, an anti-inflammatory cytokine), and osteoprotegerin (OPG) by immunohistochemistry. The first group (control) showed significantly higher expression of TNF-α and significantly lower expression of IL-10 and OPG compared to the other groups. Oral irrigation with N. oculata extracts reduced the expression of TNF-α and enhanced the expression of IL-10 and OPG in rats infected with A. actinomycetemcomitans. The effective concentration of N. oculata extract as an anti-inflammatory for oral irrigation was 2.375%.
Cross-ecosystem utilizing primed seeds of upland rice varieties for enriching crop diversity at riparian wetland during dry season
Authors: RIA ROFIQOH PURNAMA , LAKITAN BENYAMIN , SULAIMAN FIRDAUS , KARTIKA KARTIKA , SUWIGNYO RUJITO AGUS
Published: August 2020

Abstract. Ria RP, Lakitan B, Sulaiman F, Kartika K, Suwignyo RA. 2020. Cross-ecosystem utilizing primed seeds of upland rice varieties for enriching crop diversity at riparian wetland during dry season. Biodiversitas 21: 3008-3017. Cultivation of rice during second growing season at riparian wetlands in Indonesia must deal with drought conditions at reproductive stage. Seed priming can speed up seed emergence and produces vigorous seedlings. Objective of this study was to screen upland rice varieties which were positively responded to seed priming and tolerant to drought during late vegetative and/or reproductive stage. Results of this study indicated that osmo-priming showed positive effects on seed emergence, percentage of germinated seed, and time to reach 50% germination. Effects of osmo-priming on seedling growth did not go beyond four weeks after seedlings had been transplanted. Among nine varieties screened, Inpago 10 exhibited better response to seed priming during late vegetative stage as it produced the highest number of tillers and total leaf area. However, at harvest, osmo-priming with 20% PEG lowered filled spikelet and weight of 100 grains but did not affect yield. Drought during late vegetative stage lessened number of tillers but after drought-treated plants recovered during reproductive stage, percentage of filled spikelet and grain size were comparable to those of control plants. Meanwhile, drought imposed during reproductive stage decreased percentage of filled spikelet and grain yield. Despite its better performance under drought conditions, leaf rolling score was higher during heading stage in Inpago 10. This phenomenon indicated that leaf rolling was not forced by drought, rather it was a quick response of Inpago 10 variety to limit water loss due to transpiration.
Short Communication: Egg harvesting and local conservation of Moluccan Scrubfowl (Eulipoa wallacei) in the Maluku Islands, Indonesia
Authors: Simanjuntak Radios , YUSNIAR MASITAH , SAMALUKANG YUNIUS M. , BOLEU FIKTOR IMANUEL , MARDIASTUTI ANI , WIDYASARI VINCENTIA , UDIN JIHAD SULFANI
Published: August 2020

Abstract. Simanjuntak R, Yusniar M, Samalukang YM, Boleu FI, Mardiastuti A, Widyasari V, Udin JS. 2020. Short Communication: Egg harvesting and local conservation of Moluccan Scrubfowl (Eulipoa wallacei) in the Maluku Islands, Indonesia. Biodiversitas 21: 3018-3024.  This study was conducted at four locations in Central Maluku and North Halmahera, which have been used as nesting ground habitats of the Moluccan Scrubfowl (Eulipoa wallacei), an endemic bird of the Maluku Islands, Indonesia. The objective of this study was to record and analyze the egg harvesting and local conservation of Moluccan Scrubfowl in four locations in the Maluku Islands: Simau, Kao, Haruku, and Kailolo. The results showed that a relatively large number of harvested eggs were found in Simau (Halmahera Island) and Kailolo. In Kailolo, the total number of eggs harvested was 21,707 per year. The local community has the initiative to conserve this bird by establishing local regulations, called sasi. The Haruku community gave a portion of the eggs to Kewang, a customary institution authorized to manage the natural resources, to be hatched semi-naturally around the Kewang House. Likewise, the Salabia youth organization in Simau, together with landowners, developed a semi-natural hatchery for the Moluccan Scrubfowl eggs.
Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer)
Authors: Hastuti Sri Dwi , BARTON MARY D. , PYECROFT STEPHEN B. , COSTABILE MAURIZIO
Published: August 2020

Abstract. Hastuti SD, Barton MD, Pyecroft SB, Costabile M. 2020. Assay optimization for measuring the alternate complement pathway activity in Asian seabass (Lates calcarifer). Biodiversitas 21: 3034-3040. Complement proteins are one component of innate immunity present in fish. The measurement of complement activity in fish can be used to monitor the health status of fish. This is particularly important in Asian seabass (Lates calcarifer) aquaculture, where disease can impact on productivity. We have found an optimal condition assay for measuring the alternate complement pathway (ACP) activity of Asian seabass which includes Magnesium chloride (MgCl2) concentration, buffer pH, incubation temperature and incubation time. The assay was optimized using pooled serum of Asian seabass, diluted in Magnesium ethylenediamine tetraacetic acid gelatine veronal buffer (Mg-EDTA-GVB) and added with Rabbit red blood cells (RRBC) suspension. Subsequently, the suspension was incubated and centrifuged. The supernatant was removed and transferred to a well plate and the optical density (OD) was measured at 540 nm. The optimal condition obtained included a 7.5 mM MgCl2, pH optimum of 7.5, 25°C incubation temperature, and a 30 minutes incubation period. The presently developed assay was robust, rapid, and reliable to be used in monitoring the health status of Asian seabass in aquaculture farms. It can be used as guidance in further immunological studies on this fish.