bluuebunny/crossref_metadata_2025 huggingface dataset:
Restoration of Shortleaf Pine (Pinus echinata)-Hardwood Mixtures in Low Quality Mixed Upland Hardwood Stands Using Cluster Planting and Natural Regeneration
Authors: Clabo David , Clatterbuck Wayne
Published: April 2020

Cluster planting of shortleaf pine, along with various site preparation and release treatments, were tested to restore mixed shortleaf pine (Pinus echinata Mill.)–hardwood stands in areas where the shortleaf pine has diminished in recent years. Shortleaf pine–hardwood mixtures were once a common forest type throughout the Cumberland Mountains and Plateau physiographic regions of the southeastern United States. Knowledge of how to restore shortleaf pine–hardwood mixtures is limited throughout shortleaf pine’s large native range. The objectives of this study were to compare planted shortleaf pine and natural hardwood regeneration survival, growth, and composition following various site preparation and early release treatments. Cluster planting and partial timber harvesting were used to reintroduce shortleaf pine and create two-aged stands in the Cumberland Mountains of Tennessee, USA. Results indicated that shortleaf pine survival, basal diameter, and height growth did not differ following four growing seasons among treatments. Natural regeneration stem densities and heights within shortleaf pine clusters did not differ significantly by treatment. Natural regeneration stem densities differed by species group and height class across the site, while the treatment × species interaction term was also significant. At this early stage of stand development, the brown-and-burn treatment appears poised for greater shortleaf pine growth rates than the other treatments. The herbicide treatment had the fewest regenerating hardwoods per hectare and the most desirable hardwood species composition.
Linking Hydromorphological Degradation with Environmental Status of Riparian Ecosystems: A Case Study in the Stropnice River Basin, Czech Republic
Authors: Jakubínský Jiří , Pelíšek Igor , Cudlín Pavel
Published: April 2020

Recently, increasing attention has been paid to the anthropogenic degradation of the riverbed and its relationship to the ecological status of the adjacent river landscape. The key objective of this research was to determine the extent of the disturbance of the selected small streams and their riparian zone in a study area located in a forest and forest-agricultural landscape in the Czech Republic. The next step was to analyze the mutual relationships between the ecological status of the riparian vegetation and the hydromorphological status of the riverbed. The main working hypothesis considered the good hydromorphological status of the river as reflected in the favorable environmental status of the surrounding riparian habitats and vice versa. It was found in more than 90% of the total length of studied watercourses that the character of linkages between channel morphology and the ecological status of riparian vegetation is directly influenced by anthropogenic activities. An interesting finding is that the degraded streams in lowland sites are often encompassed by natural or close-to-natural habitats. On the contrary, the natural status of the riverbed was found in a significantly forested headwater area, but the riparian habitats did not reach even a close-to-natural status. This paper contributes to clarifying the significance of human impact on the river morphology, reflected in the reduction of connectivity between the terrestrial and fluvial parts of the river landscape. It helps to explore the most important disturbances affecting mutual interactions between the river and the riparian habitats.
Stem Damage Modifies the Impact of Wind on Norway Spruces
Authors: Snepsts Guntars , Kitenberga Mara , Elferts Didzis , Donis Janis , Jansons Aris
Published: April 2020

Bark stripping caused by cervids can have a long-lasting negative effect on tree vitality. Such trees of low vitality might be more susceptible to other disturbances. The amplifying effects of disturbance interactions can cause significantly more damage to forest ecosystems than the individual effects of each disturbance. Therefore, this study aimed to assess the impact of bark stripping (stem damage) on the probability of wind damage and snapping height for Norway spruces (Picea Abies (L.) H. Karst.). In this study, we used the Latvian National Forest Inventory data from the period 2004–2018. In the analysis, we used data based on 32,856 trees. To analyse the data, we implemented a Bayesian binary logistic generalised linear mixed-effects model and the linear mixed-effects model. Our results showed that stem damage significantly increased the probability of wind damage and affected the snapping height of Norway spruces. Similarly, root damage, the slenderness ratio, the stand age, the stand density, the soil type, and the dominant tree species had a significant influence on the probability of wind damage. In both periods, trees with stem damage had significantly (p < 0.05) higher probability (odd ratio 1.68) to be wind damaged than trees without stem damage. The stem damaged Norway spruce trees snapped in the first 25% of the tree height, while trees without stem damage snapped around half (50%) of the tree height. Our results show that stem damage significantly alters the effect of wind damage on Norway spruces, suggesting that such damage must be incorporated into wind-risk assessment models.
Conductive Electrospun Polyaniline/Polyvinylpyrrolidone Nanofibers: Electrical and Morphological Characterization of New Yarns for Electronic Textiles
Authors: Perdigão Patrícia , Morais Faustino Bruno Miguel , Faria Jaime , Canejo João Paulo , Borges João Paulo , Ferreira Isabel , Baptista Ana Catarina
Published: April 2020

Advanced functionalities textiles embedding electronic fibers, yarns and fabrics are a demand for innovative smart cloths. Conductive electrospun membranes and yarns based on polyaniline/polyvinylpyrrolidone (PANI/PVP) were investigated using the chemical modification of PANI instead of using conventional coating processes as in-situ polymerization. PANI was synthesized from the aniline monomer and the influence of the oxidant-to-monomer ratio on electrical conductivity was studied. The optimized conductivity of pellets made with pressed PANI powders was 21 S·cm−1. Yarns were then prepared from the t-Boc-PANI/PVP electrospun membranes followed by PANI protonation to enhance their electrical properties. Using this methodology, electrospun membranes and yarns were produced with electrical conductivities of 1.7 × 10−2 and 4.1 × 10−4 S·cm−1.
An Integrated Approach to Identify Low-Flammability Plant Species for Green Firebreaks
Authors: Murray Brad R. , Brown Colin , Murray Megan L. , Krix Daniel W. , Martin Leigh J. , Hawthorne Thomas , Wallace Molly I. , Potvin Summer A. , Webb Jonathan K.
Published: April 2020

With recent and predicted increases in the frequency and intensity of wildfires, there is a pressing need for mitigation strategies to reduce the impacts of wildfires on human lives, infrastructure and biodiversity. One strategy involves the use of low-flammability plants to build green firebreaks at the wildland–urban interface. It is common, however, to encounter uncertainty in a diverse range of stakeholders about the concept of flammability as it applies to plants, which may impede efforts to identify suitable low-flammability plant species. Here, we provide an approach to identify low-flammability plant species that integrates three fundamental and relatively easy-to-measure plant-flammability attributes – ignitibility, sustainability and combustibility – in a way that removes confusion about the concept of plant flammability. These three intrinsic flammability attributes relate to each other such that an ideal low-flammability species is one that is slow to ignite, sustains burning for a short period of time and combusts with low intensity. Consideration is then given to secondary attributes of plants critical to the selection of low-flammability plants, including attributes that influence the volume of fuel available for fires and the vertical and horizontal spread of fires. More work is urgently needed across the world to identify low-flammability plant species using standardised measurement protocols, and our integrated approach provides a transparent way to ensure we are selecting the right species, for the right location, in green firebreaks.
Numerical Simulations of Flow around Copepods: Challenges and Future Directions
Authors: Borazjani Iman
Published: April 2020

Copepods are small aquatic creatures which are abundant in oceans as a major food source for fish, thereby playing a vital role in marine ecology. Because of their role in the food chain, copepods have been subject to intense research through different perspectives from anatomy, form-function biology, to ecology. Numerical simulations can uniquely support such investigations by quantifying: (i) the force and flow generated by different parts of the body, thereby clarify the form-function relation of each part; (ii) the relation between the small-scale flow around animal and the large-scale (e.g., oceanic) flow of its surroundings; and (iii) the flow and its energetics, thereby answering ecological questions, particularly, the three major survival tasks, i.e., feeding, predator avoidance, and mate-finding. Nevertheless, such numerical simulations need to overcome challenges involving complex anatomic shape of copepods, multiple moving appendages, resolving different scales (appendage-, animal- to large-scale). The numerical methods capable of handling such problems and some recent simulations are reviewed. At the end, future developments necessary to simulate copepods from animal- to surrounding-scale are discussed.
Quality Properties of Chicken Emulsion-Type Sausages Formulated with Chicken Fatty Byproducts
Authors: Peña-Saldarriaga Lina María , Pérez-Alvarez José Angel , Fernández-López Juana
Published: April 2020

During poultry slaughtering, fatty byproducts are generated, mainly comprising abdominal and gizzard fat, which are mostly discarded and result in consequent environmental problems. The objective of this work was to use these fatty byproducts as fatty raw material in the production of chicken sausages (emulsion-type). They were applied for the partial replacement (40% and 50%) of chicken skin (fatty source usually used in chicken sausages). The effect of these partial replacements on the quality properties (proximate composition, lipid profile, color, texture, and microbiological and sensory properties) of chicken sausages were assessed. Sausages with fatty byproducts added (40% and 50%) showed lower moisture but higher fat content than control. Nevertheless, all of them meet the nutritional requirements of the Colombian regulation for this type of meat product. Sausages with 40% and 50% substitution level showed similar texture properties and microbiological quality as control. When these fatty byproducts were used at 50% substitution level, differences in several color properties and sensorial attributes (color, flavor, and meat taste) were noticed with respect to control. When the substitution level was reduced to 40%, no sensorial differences were detected. Chicken fatty byproducts can be successfully applied as partial replacement of chicken skin in emulsion-type sausages.
Influence of Grape Pomace Intake on Nutritional Value, Lipid Oxidation and Volatile Profile of Poultry Meat
Authors: Bennato Francesca , Di Luca Alessio , Martino Camillo , Ianni Andrea , Marone Elettra , Grotta Lisa , Ramazzotti Solange , Cichelli Angelo , Martino Giuseppe
Published: April 2020

Grape pomace (GP) represents the main solid by-product deriving from grape processing. The aim of this study was to evaluate the effect of dietary GP intake on nutritional quality, lipid oxidation and volatile profile of chicken meat. A total of 112 Ross 508 broilers were randomly divided into 4 groups and fed for 21 days with a standard diet. For the remaining 28 days of the trial, the control group (CG) continued to receive a standard diet, while the experimental groups (EGs) were fed with diets containing different GP concentrations: 2.5% (EG1), 5% (EG2) and 7% (EG3). Following the slaughtering, samples of breast meat were collected from each group. No significant differences were observed for pH, cooking loss and meat brightness, whereas the GP intake showed effectiveness in inducing variations in drip loss, meat yellowness and redness. The experimental feeding strategy also induced changes in the fatty acid profile, with an overall increase in polyunsaturated fatty acids (PUFA), mainly due to the increase in concentration of linoleic acid. The dietary supplementation also induced a decrease in lipid oxidation in meat, a finding also confirmed by the reduction in volatile aldehydes in 7 days stored raw meat. The feeding strategy based on the use of GP did not induce detrimental effects on the quality of broiler meat and showed the potential to lengthen the shelf-life as a direct consequence of the improvement in the oxidative stability. Overall, the present study showed a viable way for the recovery and the valorization of an agro-industrial by-product, with potential benefits also from an environmental point of view.
A Preferred Orientation Angle for Bipolar Planetary Nebulae
Authors: Ritter Andreas , Parker Quentin A.
Published: April 2020

We present preliminary results from measuring Galactic orientation angles of 766 elliptical and bipolar Planetary Nebulae (PNe) in the Hong Kong/Australian Astronomical Observatory/Strasbourg Observatory H-alpha Planetary Nebula research platform and database (HASH DB). For elliptical PNe the distribution of orientation angles is found to be more or less uniform. However, for bipolar PNe there is statistically significant evidence for preferred orientation angles (as tentatively reported before with smaller samples) across the whole Galaxy.
Synthesis and 3D Printing of Conducting Alginate–Polypyrrole Ionomers
Authors: Wright Cassandra J. , Molino Binbin Zhang , Chung Johnson H. Y. , Pannell Jonathan T. , Kuester Melissa , Molino Paul J. , Hanks Timothy W.
Published: April 2020

Hydrogels composed of calcium cross-linked alginate are under investigation as bioinks for tissue engineering scaffolds due to their variable viscoelasticity, biocompatibility, and erodibility. Here, pyrrole was oxidatively polymerized in the presence of sodium alginate solutions to form ionomeric composites of various compositions. The IR spectroscopy shows that mild base is required to prevent the oxidant from attacking the alginate during the polymerization reaction. The resulting composites were isolated as dried thin films or cross-linked hydrogels and aerogels. The products were characterized by elemental analysis to determine polypyrrole incorporation, electrical conductivity measurements, and by SEM to determine changes in morphology or large-scale phase separation. Polypyrrole incorporation of up to twice the alginate (monomer versus monomer) provided materials amenable to 3D extrusion printing. The PC12 neuronal cells adhered and proliferated on the composites, demonstrating their biocompatibility and potential for tissue engineering applications.
Utilizing Photovoice to Support Indigenous Accounts of Environmental Change and Injustice
Authors: Mitchell Felicia M. , Billiot Shanondora , Lechuga-Peña Stephanie
Published: April 2020

Global environmental changes can happen quickly or over extended periods and have compounding effects. Indigenous communities experience environmental changes that can lead to a decline in quality of life, illness or disease, and unwelcome cultural adaptations that extend to future generations. Due to limited resources and political marginalization, members of these communities may not be able to respond to or prevent these conditions. Cultural connections to the land and community, along with limited resources, impact Indigenous peoples’ willingness and ability to relocate to different geographic locations experiencing less damaging ecological changes or environmental risk. In this article, we respond to the Special Issue prompt probing “[m]ethods in which Indigenous communities engage within their environment and on the land to conduct research”. We begin by describing environmental change, followed by a scoping review of Photovoice studies focused on environmental issues. Environmental changes affecting Indigenous groups are discussed, including a case study and a discussion of the ways that Photovoice can support and honor Indigenous peoples’ connection to the natural environment. This article is not intended to be an exhaustive review, but rather seeks to understand how Photovoice is being used to respond to and document environmental change, and how such visual methodologies can be used in Indigenous communities.
Identification of Potential Biomarkers for Anti-PD-1 Therapy in Melanoma by Weighted Correlation Network Analysis
Authors: Wang Xuanyi , Chai Zixuan , Li Yinghong , Long Fei , Hao Youjin , Pan Guizhi , Liu Mingwei , Li Bo
Published: April 2020

Melanoma is the most malignant form of skin cancer, which seriously threatens human life and health. Anti-PD-1 immunotherapy has shown clinical benefits in improving patients’ overall survival, but some melanoma patients failed to respond. Effective therapeutic biomarkers are vital to evaluate and optimize benefits from anti-PD-1 treatment. Although the establishment of immunotherapy biomarkers is well underway, studies that identify predictors by gene network-based approaches are lacking. Here, we retrieved the existing datasets (GSE91061, GSE78220 and GSE93157, 79 samples in total) on anti-PD-1 therapy to explore potential therapeutic biomarkers in melanoma using weighted correlation network analysis (WGCNA), function validation and clinical corroboration. As a result, 13 hub genes as critical nodes were traced from the key module associated with clinical features. After receiver operating characteristic (ROC) curve validation by an independent dataset (GSE78220), six hub genes with diagnostic significance were further recovered. Moreover, these six genes were revealed to be closely associated not only with the immune system regulation, immune infiltration, and validated immunotherapy biomarkers, but also with excellent prognostic value and significant expression level in melanoma. The random forest prediction model constructed using these six genes presented a great diagnostic ability for anti-PD-1 immunotherapy response. Taken together, IRF1, JAK2, CD8A, IRF8, STAT5B, and SELL may serve as predictive therapeutic biomarkers for melanoma and could facilitate future anti-PD-1 therapy.
Establishment of a Recombinant AAV2/HBoV1 Vector Production System in Insect Cells
Authors: Deng Xuefeng , Zou Wei , Yan Ziying , Qiu Jianming
Published: April 2020

We have previously developed an rAAV2/HBoV1 vector in which a recombinant adeno-associated virus 2 (rAAV2) genome is pseudopackaged into a human bocavirus 1 (HBoV1) capsid. Recently, the production of rAAV2/HBoV1 in human embryonic kidney (HEK) 293 cells has been greatly improved in the absence of any HBoV1 nonstructural proteins (NS). This NS-free production system yields over 16-fold more vectors than the original production system that necessitates NS expression. The production of rAAV with infection of baculovirus expression vector (BEV) in the suspension culture of Sf9 insect cells is highly efficient and scalable. Since the replication of the rAAV2 genome in the BEV system is well established, we aimed to develop a BEV system to produce the rAAV2/HBoV1 vector in Sf9 cells. We optimized the usage of translation initiation signals of the HBoV1 capsid proteins (Cap), and constructed a BEV Bac-AAV2Rep-HBoV1Cap, which expresses the AAV2 Rep78 and Rep52 as well as the HBoV1 VP1, VP2, and VP3 at the appropriate ratios. We found that it is sufficient as a trans helper to the production of rAAV2/HBoV1 in Sf9 cells that were co-infected with the transfer Bac-AAV2ITR-GFP-luc that carried a 5.4-kb oversized rAAV2 genome with dual reporters. Further study found that incorporation of an HBoV1 small NS, NP1, in the system maximized the viral DNA replication and thus the rAAV2/HBoV1 vector production at a level similar to that of the rAAV2 vector in Sf9 cells. However, the transduction potency of the rAAV2/HBoV1 vector produced from BEV-infected Sf9 cells was 5–7-fold lower in polarized human airway epithelia than that packaged in HEK293 cells. Transmission electron microscopy analysis found that the vector produced in Sf9 cells had a high percentage of empty capsids, suggesting the pseudopackage of the rAAV2 genome in HBoV1 capsid is not as efficient as in the capsids of AAV2. Nevertheless, our study demonstrated that the rAAV2/HBoV1 can be produced in insect cells with BEVs at a comparable yield to rAAV, and that the highly efficient expression of the HBoV1 capsid proteins warrants further optimization.
MinION-Based DNA Barcoding of Preserved and Non-Invasively Collected Wildlife Samples
Authors: Seah Adeline , Lim Marisa C.W. , McAloose Denise , Prost Stefan , Seimon Tracie A.
Published: April 2020

The ability to sequence a variety of wildlife samples with portable, field-friendly equipment will have significant impacts on wildlife conservation and health applications. However, the only currently available field-friendly DNA sequencer, the MinION by Oxford Nanopore Technologies, has a high error rate compared to standard laboratory-based sequencing platforms and has not been systematically validated for DNA barcoding accuracy for preserved and non-invasively collected tissue samples. We tested whether various wildlife sample types, field-friendly methods, and our clustering-based bioinformatics pipeline, SAIGA, can be used to generate consistent and accurate consensus sequences for species identification. Here, we systematically evaluate variation in cytochrome b sequences amplified from scat, hair, feather, fresh frozen liver, and formalin-fixed paraffin-embedded (FFPE) liver. Each sample was processed by three DNA extraction protocols. For all sample types tested, the MinION consensus sequences matched the Sanger references with 99.29%–100% sequence similarity, even for samples that were difficult to amplify, such as scat and FFPE tissue extracted with Chelex resin. Sequencing errors occurred primarily in homopolymer regions, as identified in previous MinION studies. We demonstrate that it is possible to generate accurate DNA barcode sequences from preserved and non-invasively collected wildlife samples using portable MinION sequencing, creating more opportunities to apply portable sequencing technology for species identification.
Ecological Processes Affecting Long-Term Eukaryote and Prokaryote Biofilm Persistence in Nitrogen Removal from Sewage
Authors: Angell Inga Leena , Bergaust Linda , Hanssen Jon Fredrik , Aasen Else Marie , Rudi Knut
Published: April 2020

The factors affecting long-term biofilm stability in sewage treatment remain largely unexplored. We therefore analyzed moving bed bioreactors (MBBRs) biofilm composition and function two years apart from four reactors in a nitrogen-removal sewage treatment plant. Multivariate ANOVA revealed a similar prokaryote microbiota composition on biofilm carriers from the same reactors, where reactor explained 84.6% of the variance, and year only explained 1.5%. Eukaryotes showed a less similar composition with reactor explaining 56.8% of the variance and year 9.4%. Downstream effects were also more pronounced for eukaryotes than prokaryotes. For prokaryotes, carbon source emerged as a potential factor for deterministic assembly. In the two reactors with methanol as a carbon source, the bacterial genus Methylotenera dominated, with M. versatilis as the most abundant species. M. versatilis showed large lineage diversity. The lineages mainly differed with respect to potential terminal electron acceptor usage (nitrogen oxides and oxygen). Searches in the Sequence Read Archive (SRA) database indicate a global distribution of the M. versatilis strains, with methane-containing sediments as the main habitat. Taken together, our results support long-term prokaryote biofilm persistence, while eukaryotes were less persistent.
Hydrogen Isotopic Variations in the Shergottites
Authors: Wang Shuai , Hu Sen
Published: April 2020

Hydrogen isotopes in the shergottite Martian meteorites are among the most varied in Mars laboratory samples. By collating results of previous studies on major hydroxyl, deuterium, and H2O bearing phases, we provide a compendium of recent measurements in order to elucidate crustal-rock versus mantle-rock processes on Mars. We summarize recent works on volatile and δD measurements in a range of shergottite phases: from melt inclusions, apatite, merrillite, maskelynite, impact melt glass, groundmass glass, and nominal anhydrous minerals. We interpret these observations using an evidence-based approach, considering two particular scenarios: (1) water-rock crustal interactions versus (2) magmatic-based processes. We consider the implications of these measurements and the scope they have for future studies, paying particular attention to future works on H, S, and Cl isotopes in situ, shedding light on the nature of volatiles in the hydrosphere and lithosphere of Mars.
Structure of Benthic Macroinvertebrate Communities in the Rivers of Western Himalaya, Nepal
Authors: Devi Tachamo Shah Ram , Sharma Subodh , Narayan Shah Deep , Rijal Deepak
Published: April 2020

According to River Continuum Concept (RCC), channel morphology, including sediment loads and channel width, river habitat, flow regimes and water quality, differs from the tributary to the downstream river’s mainstem, allowing shifts in faunal composition from dominance of shredders to collectors downstream, respectively. Tributaries are responsible for contributing organic carbons, nutrients and water. However, such knowledge is still limited in the monsoon-dominated river systems of the Himalaya. The study was conducted in the river’s mainstem and tributaries of the Karnali River Basin, which are glacier and spring-fed river systems, respectively, in the western Himalaya, Nepal. A total of 38 river stretches in the river’s mainstem and tributaries were sampled during post-monsoon and pre-monsoon seasons in the years 2018 and 2019. Water quality parameters, such as pH, temperature, electrical conductivity, total dissolved solids, dissolved oxygen, alkalinity and hardness, and the benthic macroinvertebrates were studied. Ten subsamples of benthic macroinvertebrates were collected following the multi-habitat sampling approach at each site. High taxa richness was recorded in tributaries compared to the river’s mainstem while abundance was similar between river types. Non-metric multidimensional scaling (NMDS) formed two distinct groups, reflecting high similarities in benthic macroinvertebrate composition within the tributaries and river’s mainstem rather than between river types. Redundancy analysis (RDA) indicated water temperature and pH as major environmental predictors for benthic macroinvertebrate variability between river types. Therefore, river type-based conservation efforts that account for upstream–downstream linkages of aquatic biota and resources in freshwater ecosystems can ensure the ecological integrity of the whole river basin.
How to Relax in Stressful Situations: A Smart Stress Reduction System
Authors: Can Yekta Said , Iles-Smith Heather , Chalabianloo Niaz , Ekiz Deniz , Fernández-Álvarez Javier , Repetto Claudia , Riva Giuseppe , Ersoy Cem
Published: April 2020

Stress is an inescapable element of the modern age. Instances of untreated stress may lead to a reduction in the individual’s health, well-being and socio-economic situation. Stress management application development for wearable smart devices is a growing market. The use of wearable smart devices and biofeedback for individualized real-life stress reduction interventions has received less attention. By using our unobtrusive automatic stress detection system for use with consumer-grade smart bands, we first detected stress levels. When a high stress level is detected, our system suggests the most appropriate relaxation method by analyzing the physical activity-based contextual information. In more restricted contexts, physical activity is lower and mobile relaxation methods might be more appropriate, whereas in free contexts traditional methods might be useful. We further compared traditional and mobile relaxation methods by using our stress level detection system during an eight day EU project training event involving 15 early stage researchers (mean age 28; gender 9 Male, 6 Female). Participants’ daily stress levels were monitored and a range of traditional and mobile stress management techniques was applied. On day eight, participants were exposed to a ‘stressful’ event by being required to give an oral presentation. Insights about the success of both traditional and mobile relaxation methods by using the physiological signals and collected self-reports were provided.
Inhibition of miR-141 and miR-200a Increase DLC-1 and ZEB2 Expression, Enhance Migration and Invasion in Metastatic Serous Ovarian Cancer
Authors: Wahab Norhazlina Abdul , Othman Zahreena , Nasri Noor Wahidah Mohd , Mokhtar Mohd Helmy , Ibrahim Siti Fatimah , Hamid Adila A. , Raja Ali Raja Affendi , Mokhtar Norfilza Mohd
Published: April 2020

The role of microRNA (miRNA) in ovarian cancer has been extensively studied as a regulator for its targeted genes. However, its specific role in metastatic serous ovarian cancer (SOC) is yet to be explored. This paper aims to investigate the differentially expressed miRNAs in metastatic SOC compared to normal. Locked nucleic acid PCR was performed to profile miRNA expression in 11 snap frozen metastatic SOC and 13 normal ovarian tissues. Functional analysis and regulation of their targeted genes were assessed in vitro. Forty-eight miRNAs were significantly differentially expressed in metastatic SOC as compared to normal. MiR-19a is a novel miRNA to be upregulated in metastatic SOC compared to normal. DLC1 is possibly regulated by miR-141 in SOC. MiR-141 inhibition led to significantly reduced cell viability. Cell migration and invasion were significantly increased following miRNA inhibition. This study showed the aberrantly expressed miRNAs in metastatic SOC and the roles of miRNAs in the regulation of their targeted genes and ovarian carcinogenesis.
Investigating the Impacts of Real-Time Weather Conditions on Freeway Crash Severity: A Bayesian Spatial Analysis
Authors: Zeng Qiang , Hao Wei , Lee Jaeyoung , Chen Feng
Published: April 2020

This study presents an empirical investigation of the impacts of real-time weather conditions on the freeway crash severity. A Bayesian spatial generalized ordered logit model was developed for modeling the crash severity using the hourly wind speed, air temperature, precipitation, visibility, and humidity, as well as other observed factors. A total of 1424 crash records from Kaiyang Freeway, China in 2014 and 2015 were collected for the investigation. The proposed model can simultaneously accommodate the ordered nature in severity levels and spatial correlation across adjacent crashes. Its strength is demonstrated by the existence of significant spatial correlation and its better model fit and more reasonable estimation results than the counterparts of a generalized ordered logit model. The estimation results show that an increase in the precipitation is associated with decreases in the probabilities of light and severe crashes, and an increase in the probability of medium crashes. Additionally, driver type, vehicle type, vehicle registered province, crash time, crash type, response time of emergency medical service, and horizontal curvature and vertical grade of the crash location, were also found to have significant effects on the crash severity. To alleviate the severity levels of crashes on rainy days, some engineering countermeasures are suggested, in addition to the implemented strategies.
The Challenges of Public Health, Social Work, and Psychological Counselling Services in South Korea: The Issues of Limited Support and Resource
Authors: Dos Santos Luis Miguel
Published: April 2020

Public health, social work, and psychological counselling professions in South Korea are facing challenges of human resource shortage and shortage of professionals who can provide multilingual services. The purpose of this study was to explore and understand why public health, social work, and psychological counselling services degree graduates and professionals with multilingual skills in South Korea decide to leave their professional field to the hospitality and business industries, particularly for those who completed their initial training at one of the international universities. Based on the approach of the Social Cognitive Career Theory, individuals’ self-efficacy, outcome expectations, interests, and goals were examined and considered. The data were collected from 12 participants with the methodology of interpretative phenomenological analysis. The general inductive approach was employed to categorize the themes for reporting. The results indicated that public health, social work, and psychological counselling services-related positions are not available, modelling from peers, and lack of career development skills are the primary difficulties of public health, social work, and psychological counselling services graduates. The completion of this study provides clear recommendations to educators, policymakers, school leaders, human resource planners, and university administrators to improve their curricula and school counselling for public health, social work, and psychological counselling services graduates and the next generation.
Screening for Park Access during a Primary Care Social Determinants Screen
Authors: Razani Nooshin , Long Dayna , Hessler Danielle , Rutherford George W. , Gottlieb Laura M.
Published: April 2020

While there is evidence that access to nature and parks benefits pediatric health, it is unclear how low-income families living in an urban center acknowledge or prioritize access to parks. Methods: We conducted a study about access to parks by pediatric patients in a health system serving low-income families. Adult caregivers of pediatric patients completed a survey to identify and prioritize unmet social and economic needs, including access to parks. Univariate and multivariate analyses were conducted to explore associations between lack of access to parks and sociodemographic variables. We also explored the extent to which access to parks competed with other needs. Results: The survey was completed by 890 caregivers; 151 (17%) identified “access to green spaces/parks/playgrounds” as an unmet need, compared to 397 (45%) who endorsed “running out of food before you had money or food stamps to buy more”. Being at or below the poverty line doubled the odds (Odds ratio 1.96, 95% CI 1.16–3.31) of lacking access to a park (reference group: above the poverty line), and lacking a high school degree nearly doubled the odds. Thirty-three of the 151 (22%) caregivers who identified access to parks as an unmet need prioritized it as one of three top unmet needs. Families who faced competing needs of housing, food, and employment insecurity were less likely to prioritize park access (p < 0.001). Conclusion: Clinical interventions to increase park access would benefit from an understanding of the social and economic adversity faced by patients.
Marathon Run-induced Changes in the Erythropoietin-Erythroferrone-Hepcidin Axis are Iron Dependent
Authors: Tomczyk Maja , Kortas Jakub , Flis Damian , Kaczorowska-Hac Barbara , Grzybkowska Agata , Borkowska Andzelika , Lewicka Ewa , Dabrowska-Kugacka Alicja , Antosiewicz Jędrzej
Published: April 2020

Alterations in iron metabolism after physical activity are manifested through the rise of blood hepcidin (Hpc) levels. However, in many athletes, no changes in Hpc levels are observed after exercise despite the presence of inflammation. The missing links could be erythropoietin (EPO) and erythroferrone (ERFE), which down-regulate Hpc biosynthesis. EPO, ERFE and Hpc biosynthesis is modified by serum iron through transferrin receptor 2. Consequently, we investigated whether marathon-induced changes in EPO, ERFE and Hpc levels are blood iron-dependent. Twenty-nine healthy male marathon runners were analyzed. Serum iron, ferritin, transferrin, EPO, ERFE and Hpc levels were assessed before, immediately after, and 9 ± 2 days after the marathon. The runners whose serum Hpc decreased after the marathon (n = 15), showed a significant increase in ERFE levels. In athletes whose serum iron levels were below 105 µg/day (n = 15), serum EPO (p = 0.00) and ERFE levels (p = 0.00) increased with no changes in Hpc concentration. However, in athletes with low serum iron, no changes in EPO levels were observed when serum ferritin exceeded 70 ng/mL (n = 7). Conversely, an increase in ERFE levels was observed in marathoners with low serum iron, independently of serum ferritin (n = 7). This indicates modulation of blood iron may affect exercise-induced changes in the EPO/ERFE/Hpc axis. Further study is needed to fully understand the physiological meaning of the interdependence between iron and the EPO/ERFE/Hpc axis.
Hierarchical Variance Analysis: A Quantitative Approach for Relevant Factor Exploration and Confirmation of Perceived Tourism Impacts
Authors: Truong Quang Hai , Nguyen An Thinh , Trinh Quoc Anh , Trinh Thi Ngoc Lan , Hens Luc
Published: April 2020

The issue of tourism impacts is one that has plagued the tourism industry. This study develops a quantitative approach using hierarchical variance analysis, which deals with the exploration of the relevant factors and the confirmation of their significant contribution to analyze the residents’ perception of tourism impacts. Hierarchical variance analysis includes three mathematical procedures: Cronbach’s alpha tests, the exploration of relevant factors, and a hierarchical factor confirmation. Data are collected using a structured questionnaire completed by 452 surveyed residents living in Ly Son Island, Vietnam. The significant effects of socio-demographic variables on the overall impact assessment are observed. The bilateral and simultaneous relationships are analyzed using a one-factor ANOVA. A two-factor ANOVA shows the significant contribution of each socio-demographic variable on the economic, socio-cultural, and environmental impacts. Interaction between factors such as “Education level”, “Type of work”, etc. are hierarchically confirmed. The findings allow a better understanding of the residents’ perception of the effects of tourism on society, the economy, and the environment. This provides a scientific basis to help define problems and promote legal regulations for community participation in tourism planning in a small island destination.
Green Biased Technical Change in Terms of Industrial Water Resources in China’s Yangtze River Economic Belt
Authors: Zhang Xiyue , Sun Fangcheng , Wang Huaizu , Qu Yi
Published: April 2020

As a significant ecological corridor from west to east across China, the Yangtze River Economical Belt (YREB) is in great need of green development and transformation. Rather than only focusing on the overall growth of green productivity, it is important to identify whether the technical change is biased towards economic performance or green performance in promoting green productivity. By employing the biased technical change theory and Malmquist index decomposition method, we analyze the green biased technical change in terms of industrial water resources in YREB at the output side and the input side respectively. We find that the green biased technical change varies during 2006–2015 at both the input side and output side in YREB. At the input side, water-saving biased technical change is generally dominant compared to water-using biased technical change during 2006–2015, presenting the substitution effects of non-water production factors. At the output side, the economy-growth biased technical change is the main force to promote green productivity, whereas the role of water-conservation biased technical change is insufficient. The green performance at the output side needs to be strengthened compared to the economic performance in YREB. A series of water-related environmental policies introduced in China since 2008 have promoted the green biased technical change both at the input side and the output side in YREB, but the policy effects at the output side is still inadequate compared to that at the input side. The technological innovation in sewage treatment and control need to catch up with the economic growth in YREB. Our research gives insights to enable a deeper understanding of the green biased technical change in YREB and will benefit more focused policy-making of green innovation.
Dynamic Balance, but Not Precision Throw, Is Positively Associated with Academic Performance in Children
Authors: Lima Rodrigo A. , Stodden David F. , Pfeiffer Karin A. , Larsen Lisbeth R. , Barros Mauro V. G. , Bugge Anna , Andersen Lars B.
Published: April 2020

We analyzed the longitudinal association between dynamic balance and throwing skill with academic performance and whether waist circumference mediated these relationships. The current one-year longitudinal study followed 1020 first (mean age 7.87 ± 0.34 years) through fifth grade (mean age 11.87 ± 0.37 years) children, measured twice in 2010 and 2011. Dynamic balance and precision throw were measures of motor competence. Waist circumference was measured with a measuring tape at the umbilicus level. Academic performance was assessed by a combined score of standardized Danish language and math tests. Structural equation modeling was used for analysis. All coefficients are standardized. Balance was associated with academic performance when both sexes were combined (β = 0.126, 95% CI: 0.074 to 0.179), and waist circumference partially mediated the relationship (β = 0.021, 95% CI: 0.008 to 0.034). For boys, balance was associated with academic performance, but waist circumference did not mediate the association. For girls, balance presented direct, mediated (via waist circumference), and total associations with academic performance. Dynamic balance is an important gross motor function that was longitudinally related to academic performance, and waist circumference partially mediated the relationship. Precision throw was not found to be associated with academic performance in both sexes combined or when analyzed separately.
Modification of PARP4, XRCC3, and RAD51 Gene Polymorphisms on the Relation between Bisphenol A Exposure and Liver Abnormality
Authors: Kim Jin Hee , Hong Yun-Chul
Published: April 2020

Repair genes may play critical roles in the relationships between environmental exposure and health outcomes. However, no evidence is available about the effect of repair gene polymorphisms on the relationship between bisphenol A (BPA) exposure and liver abnormality. Therefore, we evaluated the effect of nine genotyped polymorphisms in three repair genes, poly(ADP-ribose) polymerase family member 4 (PARP4), X-ray repair cross complementing 3 (XRCC3), and RAD51 recombinase (RAD51), on the relationship between BPA exposure and liver abnormality using repeated measures data for an elderly population. A significant association between BPA levels and liver abnormality was found only in elders with the PARP4 G-C-G haplotype, XRCC3 G-A-G haplotype, or RAD51 T-A-A haplotype (odds ratio (OR) = 2.16 and p = 0.0014 for PARP4; OR = 1.57 and p = 0.0249 for XRCC3; OR = 1.43 and p = 0.0422 for RAD51). Particularly, PARP4 and XRCC3 showed significant interactions with BPA exposure in relation to liver abnormality (p < 0.05 for both genes). These results indicate that PARP4, XRCC3, and RAD51 gene polymorphisms have modification effects on the relationship between BPA exposure and liver abnormality.
Can a Community-Led Intervention Offering Social Support and Health Education Improve Maternal Health? A Repeated Measures Evaluation of the PACT Project Run in a Socially Deprived London Borough
Authors: Brown June , Luderowski Ana , Namusisi-Riley Josephine , Moore-Shelley Imogen , Bolton Matthew , Bolton Derek
Published: April 2020

Social adversity can significantly influence the wellbeing of mothers and their children. Maternal health may be improved through strengthened support networks and better health literacy. Health improvement at the population level requires optimizing of the collaboration between statutory health services, civic organizations (e.g., churches, schools), as well as community groups and parents. Two key elements in improving community engagement are co-production and community control. This study evaluated a co-produced and community-led project, PACT (Parents and Communities Together), for mothers in a deprived south London borough. The project offered social support and health education. Intended effects were improvements in mental health, health literacy, and social support, assessed by standardized measures in a pre-post design. Sixty-one mothers consented to take part in the evaluation. Significant improvements were found in mental health measures, in health literacy, for those with low literacy at baseline, and in overall and some specific aspects of social support. Satisfaction with the project was high. We found that the project engaged local populations that access statutory health services relatively less. We conclude that community-organized and community-led interventions in collaboration with statutory health services can increase accessibility and can improve mothers’ mental health and other health-related outcomes.
Development of an Assessment Method for Investigating the Impact of Climate and Urban Parameters in Confirmed Cases of COVID-19: A New Challenge in Sustainable Development
Authors: Pirouz Behrouz , Shaffiee Haghshenas Sina , Pirouz Behzad , Shaffiee Haghshenas Sami , Piro Patrizia
Published: April 2020

Sustainable development has been a controversial global topic, and as a complex concept in recent years, it plays a key role in creating a favorable future for societies. Meanwhile, there are several problems in the process of implementing this approach, like epidemic diseases. Hence, in this study, the impact of climate and urban factors on confirmed cases of COVID-19 (a new type of coronavirus) with the trend and multivariate linear regression (MLR) has been investigated to propose a more accurate prediction model. For this propose, some important climate parameters, including daily average temperature, relative humidity, and wind speed, in addition to urban parameters such as population density, were considered, and their impacts on confirmed cases of COVID-19 were analyzed. The analysis was performed for three case studies in Italy, and the application of the proposed method has been investigated. The impacts of parameters have been considered with a delay time from one to nine days to find out the most suitable combination. The result of the analysis demonstrates the effectiveness of the proposed model and the impact of climate parameters on the trend of confirmed cases. The research hypothesis approved by the MLR model and the present assessment method could be applied by considering several variables that exhibit the exact delay of them to new confirmed cases of COVID-19.