bluuebunny/crossref_metadata_2025 huggingface dataset:
High Success Rate of Prehospital and En Route Cricothyroidotomy Performed in the Israel Defense Forces: 20 Years of Experience
Authors: Beit Ner Eran , Tsur Avishai M. , Nadler Roy , Glassberg Elon , Benov Avi , Chen Jacob
Published: November 2021

Introduction:

Securing the airway is a crucial stage of trauma care. Cricothyroidotomy (CRIC) is often addressed as a salvage procedure in complicated cases or following a failed endotracheal intubation (ETI). Nevertheless, it is a very important skill in prehospital settings, such as on the battlefield.

Hypothesis/Problem:

This study aimed to review the Israel Defense Forces (IDF) experience with CRIC over the past two decades.

Methods:

The IDF Trauma Registry (IDF-TR) holds data on all trauma casualties (civilian and military) cared for by military medical teams since 1997. Data of all casualties treated by IDF from 1998 through 2018 were extracted and analyzed to identify all patients who underwent CRIC procedures.

Variables describing the incident scenario, patient’s characteristics, injury pattern, treatment, and outcome were extracted. The success rate of the procedure was described, and selected variables were further analyzed and compared using the Fisher’s-exact test to identify their effect on the success and failure rates. Odds Ratio (OR) was further calculated for the effect of different body part involvement on success and for the mortality after failed ETI.

Results:

One hundred fifty-three casualties on which a CRIC attempt was made were identified from the IDF-TR records. The overall success rate of CRIC was reported at 88%. In patients who underwent one or two attempts, the success rate was 86%. No difference was found across providers (physician versus paramedic). The CRIC success rates for casualties with and without head trauma were 80% and 92%, respectively (P = .06). Overall mortality was 33%.

Conclusions:

This study shows that CRIC is of merit in airway management as it has shown to have consistently high success rates throughout different levels of training, injuries, and previous attempts with ETI. Care providers should be encouraged to retain and develop this skill as part of their tool box.
Knowledge is a river and education is like a stairway: An eye movement study on how L2 speakers process metaphors and similes
Authors: Olkoniemi Henri , Bertram Raymond , Kaakinen Johanna K.
Published: November 2021

Very little is known about the processes underlying second language (L2) speakers’ understanding of written metaphors and similes. Moreover, most of the theories on figurative language comprehension do not consider reader-related factors. In the study, we used eye-tracking to examine how native Finnish speakers (N = 63) read written English nominal metaphors (“education is a stairway”) and similes (“education is like a stairway”). Identical topic–vehicle pairs were used in both conditions. After reading, participants evaluated familiarity of each pair. English proficiency was measured using the Bilingual-language Profile Questionnaire and the Lexical Test for Advanced Learners of English. The results showed that readers were more likely to regress within metaphors than within similes, indicating that processing metaphors requires more processing effort than processing similes. The familiarity of a metaphor and L2 English proficiency modulated this effect. The results are discussed in the light of current theories on figurative language processing.
Predictors of language proficiency in school-age Spanish–English bilingual children with and without developmental language disorder
Authors: Ebert Kerry Danahy , Reilly Madeline
Published: November 2021

Children with developmental language disorder (DLD) have impairments in their language-learning abilities that may influence interactions with environmental opportunities to learn two languages. This study explores relationships between proficiency in L1 and L2 and a set of environmental and personal variables within a group of school-age Spanish–English bilingual children with DLD and a group of typically-developing peers. Within each group, current usage in the home, length of L2 exposure, gender, maternal education, analytical reasoning, and number of L1 conversational partners were used to predict proficiency in each language. Results showed that home language environment, particularly home L2 usage, strongly predicted L1 proficiency but had less influence on the L2. Female gender predicted L1 skills in both groups, whereas analytical reasoning predicted both L1 and L2 but only for children with DLD. This study expands the limited literature on how children with DLD interact with their environment to learn two languages.
Validitätsbefunde zum Bochumer Inventar zur berufsbezogenen Persönlichkeitsbeschreibung (BIP)
Authors: Merchel Robin , Frieg Philip , Hossiep Rüdiger
Published: November 2021

Zusammenfassung. Das Bochumer Inventar zur berufsbezogenen Persönlichkeitsbeschreibung (BIP) erfasst berufsbezogene Persönlichkeitsmerkmale und kann in linearen Regressionen verschiedene Maße subjektiven und objektiven Berufserfolgs aufklären. Um zusätzliche Nachweise für die Kriteriumsvalidität zu erbringen, werden in der vorliegenden Arbeit Cluster- und Klassifikationsverfahren verwendet. Mithilfe von k-Means-Clusteranalysen können typische Persönlichkeitsstrukturen identifiziert werden: Personen, die sich durch Flexibilität und Gestaltungsmotivation auszeichnen, weisen einen bedeutsamen Zusammenhang zu höheren beruflichen Entgelten auf, während solche, die durch emotionale Instabilität und geringe Durchsetzungsstärke geprägt sind, häufig ein niedriges Entgelt erzielen. Klassische und neuere Klassifikationsverfahren (logistische Regressionen bzw. Random Forests) besitzen substantielle Trefferquoten in der Identifikation von Mitarbeitenden als Fach- oder Führungskraft. Die Ergebnisse sind als mittlere bis große Effekte einzustufen und liefern damit einen Nachweis über die Relevanz der Persönlichkeit für beruflichen Erfolg.
Moving Toward L‐Band NASA‐ISRO SAR Mission (NISAR) Dense Time Series: Multipolarization Object‐Based Classification of Wetlands Using Two Machine Learning Algorithms
Authors: Adeli Sarina , Salehi Bahram , Mahdianpari Masoud , Quackenbush Lindi J. , Chapman Bruce
Published: November 2021

Given the key role wetlands play in climate regulation and shoreline stabilization, identifying their spatial distribution is essential for the management, restoration, and protection of these invaluable ecosystems. The increasing availability of high spatial and temporal resolution optical and synthetic aperture radar (SAR) remote sensing data coupled with advanced machine learning techniques have provided an unprecedented opportunity for mapping complex wetlands’ ecosystems. A recent partnership between the National Aeronautics and Space Administration (NASA) and the Indian Space Research Organization (ISRO) resulted in the design of the NASA‐ISRO SAR (NISAR) mission. In this study, the capability of L‐band simulated NISAR data for wetland mapping in Yucatan Lake, Louisiana, is investigated using two object‐based machine learning approaches: Support vector machine (SVM) and random forest (RF). L‐band Unmanned Aerial Vehicle SAR (UAVSAR) data are exploited as a proxy for NISAR data. Specifically, we evaluated the synergistic use of different polarimetric features for efficient delineation of wetland types, extracting 84 polarimetric features from more than 10 polarimetric decompositions. High spatial resolution National Agriculture Imagery Program imagery is applied for image segmentation using the mean‐shift algorithm. Overall accuracies of 74.33% and 81.93% obtained by SVM and RF, respectively, demonstrate the great possibility of L‐band prototype NISAR data for wetland mapping and monitoring. In addition, variable importance analysis using the Gini index for RF classifier suggests that H/A/ALPHA, Freeman‐Durden, and Aghababaee features have the highest contribution to the overall accuracy.
Mapping and Monitoring of DNAPL Source Zones With Combined Direct Current Resistivity and Induced Polarization: A Field‐Scale Numerical Investigation
Authors: Almpanis Angelos , Gerhard Jason , Power Christopher
Published: November 2021

Direct current (DC) resistivity has been widely investigated for non‐invasive mapping of dense non‐aqueous phase liquids (DNAPLs); however, due to its difficulty in distinguishing DNAPLs from adjacent soils, the DC method is limited for static detection of DNAPLs and more often employed for monitoring DNAPL changes over time. Time‐domain induced polarization (IP) can provide complementary information to better discriminate between DNAPL, water and surrounding soils. Since highly resistive DNAPLs tend to laterally spread and pool on polarizable (chargeable) clay lenses, combined DC and IP (DCIP) has the potential to enhance static detection, and also monitoring of DNAPL source zones (SZs). The objective of this study is to assess DCIP for characterizing and monitoring DNAPL SZs at the field‐scale. A new DNAPL‐DCIP numerical model was first developed that couples a 3D multiphase flow model, which simulates DNAPL release and remediation scenarios, with a 3D DCIP model, which calculates the corresponding resistivity and chargeability response. The sensitivity of the DCIP response to key DNAPL and soil properties was then analyzed at a single subsurface location, closely matching previous theoretical and experimental observations. Finally, a field‐scale simulation of DNAPL release and remediation was conducted, with simultaneous mapping by DCIP surveys. Results demonstrate that chargeability can provide enhanced understanding of the lithological distribution that controls the variability in the DNAPL SZ, with time‐lapse resistivity being used to monitor DNAPL mass changes during SZ remediation. This numerical study suggests that combined DCIP can be valuable for site characterization and time‐lapse monitoring performance at DNAPL‐impacted sites.
Multiple metals in children’s deciduous teeth: results from a community-initiated pilot study
Authors: Friedman Alexa , Bauer Julia Anglen , Austin Christine , Downs Timothy J. , Tripodis Yorghos , Heiger-Bernays Wendy , White Roberta F. , Arora Manish , Claus Henn Birgit
Published: November 2021

Background

Characterizing retrospective exposure to toxicants during multiple early-life developmental periods is challenging, yet critical for understanding developmental effects.

Objective

To characterize early-life metal exposure using deciduous teeth in a community concerned about past exposures.

Methods

Naturally shed teeth were collected from 30 children ages 5–13 years who resided in Holliston, Massachusetts since conception. We estimated weekly prenatal and postnatal (up to 1 year of age) exposure to 12 metals by measuring dentine concentrations using laser ablation-inductively coupled plasma-mass spectrometry. Multivariable linear mixed models were used to explore sociodemographic, dietary, and behavioral correlates of dentine metal concentrations.

Results

Temporal trends in dentine levels differed by metal. Source of milk during the first year of life was associated with dentine barium (Ba) levels, where being fed predominantly breastmilk was associated with 39% (95% CI: –57%, –13%) lower dentine Ba compared to predominantly formula use. Females had higher prenatal and postnatal dentine Mn and Pb, compared to males (e.g., % difference, postnatal Mn: 122% (17%, 321%); postnatal Pb: 60% (95% CI: –8%, 178%)).

Significance

Deciduous teeth provide retrospective information on dose and timing of early-life metals exposure at high resolution. We demonstrate their utility in a community-based study with known past contamination of drinking water.

Impact statement

We conducted a community-initiated pilot study in a community concerned with historical exposure to multiple metals. Using deciduous teeth, a novel noninvasive biomarker, we characterized early-life exposure to 12 metals in approximately weekly increments during sensitive developmental periods, thus demonstrating the utility of this biomarker in communities concerned with past exposures.
Over-representation of potential SP4 target genes within schizophrenia-risk genes
Authors: Zhou Xianjin
Published: November 2021

Reduction of Sp4 expression causes age-dependent hippocampal vacuolization and many other intermediate phenotypes of schizophrenia in Sp4 hypomorphic mice. Recent human genetic studies from both the Schizophrenia Exome Sequencing Meta-Analysis (SCHEMA) and the Genome-Wide Association Study (GWAS) validated SP4 as a schizophrenia-risk gene over the exome-wide or the genome-wide significance. Truncation of the human SP4 gene has an odds ratio of 9.37 (3.38–29.7) for schizophrenia. Despite successful identification of many schizophrenia-risk genes, it is unknown whether and how these risk genes may interact with each other in the development of schizophrenia. By taking advantage of the specific localization of the GC-boxes bound by SP4 transcription factors, I analyzed the relative abundance of these GC-boxes in the proximal promoter regions of schizophrenia-risk genes. I found that the GC-box containing genes are significantly over-represented within schizophrenia-risk genes, suggesting that SP4 is not only a high-risk gene for schizophrenia, but may also act as a hub of network in the regulation of many other schizophrenia-risk genes via these GC-boxes in the pathogenesis of schizophrenia.
Cancer biology and molecular genetics of A3 adenosine receptor
Authors: Mazziotta Chiara , Rotondo John Charles , Lanzillotti Carmen , Campione Giulia , Martini Fernanda , Tognon Mauro
Published: November 2021

A3 adenosine receptor (A3AR) is a cell membrane protein, which has been found to be overexpressed in a large number of cancer types. This receptor plays an important role in cancer by interacting with adenosine. Specifically, A3AR has a dual nature in different pathophysiological conditions, as it is expressed according to tissue type and stimulated by an adenosine dose-dependent manner. A3AR activation leads to tumor growth, cell proliferation and survival in some cases, while triggering cytostatic and apoptotic pathways in others. This review aims to describe the most relevant aspects of A3AR activation and its ligands whereas it summarizes A3AR activities in cancer. Progress in the field of A3AR modulators, with a potential therapeutic role in cancer treatment are reported, as well.
Proteomics analysis of the gut–brain axis in a gut microbiota-dysbiosis model of depression
Authors: Liu Yiyun , Wang Haiyang , Gui Siwen , Zeng Benhua , Pu Juncai , Zheng Peng , Zeng Li , Luo Yuanyuan , Wu You , Zhou Chanjuan , Song Jinlin , Ji Ping , Wei Hong , Xie Peng
Published: November 2021

Major depressive disorder (MDD) is a serious mental illness. Increasing evidence from both animal and human studies suggested that the gut microbiota might be involved in the onset of depression via the gut–brain axis. However, the mechanism in depression remains unclear. To explore the protein changes of the gut–brain axis modulated by gut microbiota, germ-free mice were transplanted with gut microbiota from MDD patients to induce depression-like behaviors. Behavioral tests were performed following fecal microbiota transplantation. A quantitative proteomics approach was used to examine changes in protein expression in the prefrontal cortex (PFC), liver, cecum, and serum. Then differential protein analysis and weighted gene coexpression network analysis were used to identify microbiota-related protein modules. Our results suggested that gut microbiota induced the alteration of protein expression levels in multiple tissues of the gut–brain axis in mice with depression-like phenotype, and these changes of the PFC and liver were model specific compared to chronic stress models. Gene ontology enrichment analysis revealed that the protein changes of the gut–brain axis were involved in a variety of biological functions, including metabolic process and inflammatory response, in which energy metabolism is the core change of the protein network. Our data provide clues for future studies in the gut–brain axis on protein level and deepen the understanding of how gut microbiota cause depression-like behaviors.
Long non-coding RNAs are involved in alternative splicing and promote cancer progression
Authors: Ouyang Jiawei , Zhong Yu , Zhang Yijie , Yang Liting , Wu Pan , Hou Xiangchan , Xiong Fang , Li Xiayu , Zhang Shanshan , Gong Zhaojian , He Yi , Tang Yanyan , Zhang Wenling , Xiang Bo , Zhou Ming , Ma Jian , Li Yong , Li Guiyuan , Zeng Zhaoyang , Guo Can , Xiong Wei
Published: November 2021

Alternative splicing (AS) is a key process in which precursor RNAs produce different mature RNAs, and the disorder of AS is a key factor in promoting cancer development. Compared with coding RNA, studies on the functions of long non-coding RNAs (lncRNAs) are far from enough. In fact, lncRNA is an important participant and regulator in the process of AS. On the one hand, lncRNAs regulate cancer progression as AS products of precursor messenger RNA (mRNA), but on the other hand, precursor lncRNA generates cancer-related abnormal splicing variants through AS. In addition, lncRNAs directly or indirectly regulate the AS events of downstream target genes, thus affecting the occurrence and development of cancer. Here, we reviewed how lncRNAs regulate AS and influence oncogenesis in different ways.
Bcl-xL acts as an inhibitor of IP3R channels, thereby antagonizing Ca2+-driven apoptosis
Authors: Rosa Nicolas , Ivanova Hristina , Wagner Larry E. , Kale Justin , La Rovere Rita , Welkenhuyzen Kirsten , Louros Nikolaos , Karamanou Spyridoula , Shabardina Victoria , Lemmens Irma , Vandermarliere Elien , Hamada Kozo , Ando Hideaki , Rousseau Frederic , Schymkowitz Joost , Tavernier Jan , Mikoshiba Katsuhiko , Economou Anastassios , Andrews David W. , Parys Jan B. , Yule David I. , Bultynck Geert
Published: November 2021

Anti-apoptotic Bcl-2-family members not only act at mitochondria but also at the endoplasmic reticulum, where they impact Ca2+ dynamics by controlling IP3 receptor (IP3R) function. Current models propose distinct roles for Bcl-2 vs. Bcl-xL, with Bcl-2 inhibiting IP3Rs and preventing pro-apoptotic Ca2+ release and Bcl-xL sensitizing IP3Rs to low [IP3] and promoting pro-survival Ca2+ oscillations. We here demonstrate that Bcl-xL too inhibits IP3R-mediated Ca2+ release by interacting with the same IP3R regions as Bcl-2. Via in silico superposition, we previously found that the residue K87 of Bcl-xL spatially resembled K17 of Bcl-2, a residue critical for Bcl-2’s IP3R-inhibitory properties. Mutagenesis of K87 in Bcl-xL impaired its binding to IP3R and abrogated Bcl-xL’s inhibitory effect on IP3Rs. Single-channel recordings demonstrate that purified Bcl-xL, but not Bcl-xLK87D, suppressed IP3R single-channel openings stimulated by sub-maximal and threshold [IP3]. Moreover, we demonstrate that Bcl-xL-mediated inhibition of IP3Rs contributes to its anti-apoptotic properties against Ca2+-driven apoptosis. Staurosporine (STS) elicits long-lasting Ca2+ elevations in wild-type but not in IP3R-knockout HeLa cells, sensitizing the former to STS treatment. Overexpression of Bcl-xL in wild-type HeLa cells suppressed STS-induced Ca2+ signals and cell death, while Bcl-xLK87D was much less effective in doing so. In the absence of IP3Rs, Bcl-xL and Bcl-xLK87D were equally effective in suppressing STS-induced cell death. Finally, we demonstrate that endogenous Bcl-xL also suppress IP3R activity in MDA-MB-231 breast cancer cells, whereby Bcl-xL knockdown augmented IP3R-mediated Ca2+ release and increased the sensitivity towards STS, without altering the ER Ca2+ content. Hence, this study challenges the current paradigm of divergent functions for Bcl-2 and Bcl-xL in Ca2+-signaling modulation and reveals that, similarly to Bcl-2, Bcl-xL inhibits IP3R-mediated Ca2+ release and IP3R-driven cell death. Our work further underpins that IP3R inhibition is an integral part of Bcl-xL’s anti-apoptotic function.
CD5L deficiency attenuate acetaminophen-induced liver damage in mice via regulation of JNK and ERK signaling pathway
Authors: Li Mengjing , Ling Tao , Teng Fengmeng , Hu Chao , Su Zhongping , Zhang Chen , Li Xiang , Zhao Ting , Mu Xianmin , Li Yingchang , Pan Jinshun , You Qiang
Published: November 2021

CD5 molecule like (CD5L), a member of the scavenger receptor cysteine-rich domain superfamily, plays a critical role in immune homeostasis and inflammatory disease. Acetaminophen (APAP) is a safe and effective antipyretic analgesic. However, overdose may cause liver damage or even liver failure. APAP hepatotoxicity is characterized by extensive necrotic cell death and a sterile inflammatory response, in which the role of CD5L remains to be investigated. In this study, we found that the expression of CD5L was increased in the livers of mice after APAP overdose. Furthermore, CD5L deficiency reduced the increase of alanine transaminase (ALT) level, histopathologic lesion area, c-Jun N-terminal kinase (JNK)/extracellular signal-regulated kinase (ERK) phosphorylation level, Transferase-Mediated dUTP Nick End-Labeling positive (TUNEL+) cells proportion, vascular endothelial cell permeability and release of inflammatory cytokines induced by excess APAP. Therefore, our findings reveal that CD5L may be a potential therapeutic target for prevention and treatment of APAP-induced liver injury.
Glass transition temperature prediction of disordered molecular solids
Authors: Lin Kun-Han , Paterson Leanne , May Falk , Andrienko Denis
Published: November 2021

Glass transition temperature, Tg, is the key quantity for assessing morphological stability and molecular ordering of films of organic semiconductors. A reliable prediction of Tg from the chemical structure is, however, challenging, as it is sensitive to both molecular interactions and analysis of the heating or cooling process. By combining a fitting protocol with an automated workflow for forcefield parameterization, we predict Tg with a mean absolute error of ~20 °C for a set of organic compounds with Tg in the 50–230 °C range. Our study establishes a reliable and automated prescreening procedure for the design of amorphous organic semiconductors, essential for the optimization and development of organic light-emitting diodes.
Electron–plasmon and electron–magnon scattering in ferromagnets from first principles by combining GW and GT self-energies
Authors: Nabok Dmitrii , Blügel Stefan , Friedrich Christoph
Published: November 2021

This work combines two powerful self-energy techniques: the well-known GW method and a self-energy recently developed by us that describes renormalization effects caused by the scattering of electrons with magnons and Stoner excitations. This GT self-energy, which is fully k-dependent and contains infinitely many spin-flip ladder diagrams, was shown to have a profound impact on the electronic band structure of Fe, Co, and Ni. In the present work, we refine the method by combining GT with the GW self-energy. The resulting GWT spectral functions exhibit strong lifetime effects and emergent dispersion anomalies. They are in an overall better agreement with experimental spectra than those obtained with GW or GT alone, even showing partial improvements over local-spin-density approximation dynamical mean-field theory. The performed analysis provides a basis for applying the GWT technique to a wider class of magnetic materials.
Development of molecular clamp stabilized hemagglutinin vaccines for Influenza A viruses
Authors: McMillan Christopher L. D. , Cheung Stacey T. M. , Modhiran Naphak , Barnes James , Amarilla Alberto A. , Bielefeldt-Ohmann Helle , Lee Leo Yi Yang , Guilfoyle Kate , van Amerongen Geert , Stittelaar Koert , Jakob Virginie , Lebas Celia , Reading Patrick , Short Kirsty R. , Young Paul R. , Watterson Daniel , Chappell Keith J.
Published: November 2021

Influenza viruses cause a significant number of infections and deaths annually. In addition to seasonal infections, the risk of an influenza virus pandemic emerging is extremely high owing to the large reservoir of diverse influenza viruses found in animals and the co-circulation of many influenza subtypes which can reassort into novel strains. Development of a universal influenza vaccine has proven extremely challenging. In the absence of such a vaccine, rapid response technologies provide the best potential to counter a novel influenza outbreak. Here, we demonstrate that a modular trimerization domain known as the molecular clamp allows the efficient production and purification of conformationally stabilised prefusion hemagglutinin (HA) from a diverse range of influenza A subtypes. These clamp-stabilised HA proteins provided robust protection from homologous virus challenge in mouse and ferret models and some cross protection against heterologous virus challenge. This work provides a proof-of-concept for clamp-stabilised HA vaccines as a tool for rapid response vaccine development against future influenza A virus pandemics.
Pd immobilization biguanidine modified Zr-UiO-66 MOF as a reusable heterogeneous catalyst in Suzuki–Miyaura coupling
Authors: Veisi Hojat , Abrifam Mozhdeh , Kamangar Sheida Ahany , Pirhayati Mozhgan , Saremi Shokoufeh Ghahri , Noroozi Mohammad , Tamoradi Taiebeh , Karmakar Bikash
Published: November 2021

In recent days, nanohybrid metal organic frameworks (MOF) have been considered as next generation catalysts due to their unique features like large surface to volume ratio, tailorable geometry, uniform pore sizes and homogeneous distribution of active sites. In this report, we address the biguanidine modified 3D Zr-centred MOF UiO-66-NH2 following a post synthetic modification approach. Utilizing the excellent chelating ability of biguanidine, Pd ions are immobilized over the host matrix MOF. The as-synthesized material was physicochemically characterized using a broad range of analytical techniques like FT-IR, electron microscopy, EDS, elemental mapping, XRD and ICP-OES. Subsequently the material has been catalytically employed in the classical Suzuki–Miyaura coupling towards the synthesis of diverse biphenyl derivatives at sustainable conditions. There are very few reports on the covalently modified MOFs towards the organic coupling reactions. The catalyst has been isolated by centrifugation and recycled in 9 consecutive runs with almost insignificant leaching and minute decrease in reactivity.
Effects of mobility restrictions during COVID19 in Italy
Authors: Smolyak Alex , Bonaccorsi Giovanni , Flori Andrea , Pammolli Fabio , Havlin Shlomo
Published: November 2021

To reduce the spread and the effect of the COVID-19 global pandemic, non-pharmaceutical interventions have been adopted on multiple occasions by governments. In particular lockdown policies, i.e., generalized mobility restrictions, have been employed to fight the first wave of the pandemic. We analyze data reflecting mobility levels over time in Italy before, during and after the national lockdown, in order to assess some direct and indirect effects. By applying methodologies based on percolation and network science approaches, we find that the typical network characteristics, while very revealing, do not tell the whole story. In particular, the Italian mobility network during lockdown has been damaged much more than node- and edge-level metrics indicate. Additionally, many of the main Provinces of Italy are affected by the lockdown in a surprisingly similar fashion, despite their geographical and economic dissimilarity. Based on our findings we offer an approach to estimate unavailable high-resolution economic dimensions, such as real time Province-level GDP, based on easily measurable mobility information.
Brescia-COVID Respiratory Severity Scale (BRCSS) and Quick SOFA (qSOFA) score are most useful in showing severity in COVID-19 patients
Authors: San Ishak , Gemcioglu Emin , Baser Salih , Yilmaz Cakmak Nuray , Erden Abdulsamet , Izdes Seval , Catalbas Ramis , Davutoglu Mehmet , Karabuga Berkan , Ates Ihsan
Published: November 2021

In this study, we compare the predictive value of clinical scoring systems that are already in use in patients with Coronavirus disease 2019 (COVID-19), including the Brescia-COVID Respiratory Severity Scale (BCRSS), Quick SOFA (qSOFA), Sequential Organ Failure Assessment (SOFA), Multilobular infiltration, hypo-Lymphocytosis, Bacterial coinfection, Smoking history, hyper-Tension, and Age (MuLBSTA) and scoring system for reactive hemophagocytic syndrome (HScore), for determining the severity of the disease. Our aim in this study is to determine which scoring system is most useful in determining disease severity and to guide clinicians. We classified the patients into two groups according to the stage of the disease (severe and non-severe) and adopted interim guidance of the World Health Organization. Severe cases were divided into a group of surviving patients and a deceased group according to the prognosis. According to admission values, the BCRSS, qSOFA, SOFA, MuLBSTA, and HScore were evaluated at admission using the worst parameters available in the first 24 h. Of the 417 patients included in our study, 46 (11%) were in the severe group, while 371 (89%) were in the non-severe group. Of these 417 patients, 230 (55.2%) were men. The median (IQR) age of all patients was 44 (25) years. In multivariate logistic regression analyses, BRCSS in the highest tertile (HR 6.1, 95% CI 2.105–17.674, p = 0.001) was determined as an independent predictor of severe disease in cases of COVID-19. In multivariate analyses, qSOFA was also found to be an independent predictor of severe COVID-19 (HR 4.757, 95% CI 1.438–15.730, p = 0.011). The area under the curve (AUC) of the BRCSS, qSOFA, SOFA, MuLBSTA, and HScore was 0.977, 0.961, 0.958, 0.860, and 0.698, respectively. Calculation of the BRCSS and qSOFA at the time of hospital admission can predict critical clinical outcomes in patients with COVID-19, and their predictive value is superior to that of HScore, MuLBSTA, and SOFA. Our prediction is that early interventions for high-risk patients, with early identification of high-risk group using BRCSS and qSOFA, may improve clinical outcomes in COVID-19.
Fully-automated atrophy segmentation in dry age-related macular degeneration in optical coherence tomography
Authors: Derradji Yasmine , Mosinska Agata , Apostolopoulos Stefanos , Ciller Carlos , De Zanet Sandro , Mantel Irmela
Published: November 2021

Age-related macular degeneration (AMD) is a progressive retinal disease, causing vision loss. A more detailed characterization of its atrophic form became possible thanks to the introduction of Optical Coherence Tomography (OCT). However, manual atrophy quantification in 3D retinal scans is a tedious task and prevents taking full advantage of the accurate retina depiction. In this study we developed a fully automated algorithm segmenting Retinal Pigment Epithelial and Outer Retinal Atrophy (RORA) in dry AMD on macular OCT. 62 SD-OCT scans from eyes with atrophic AMD (57 patients) were collected and split into train and test sets. The training set was used to develop a Convolutional Neural Network (CNN). The performance of the algorithm was established by cross validation and comparison to the test set with ground-truth annotated by two graders. Additionally, the effect of using retinal layer segmentation during training was investigated. The algorithm achieved mean Dice scores of 0.881 and 0.844, sensitivity of 0.850 and 0.915 and precision of 0.928 and 0.799 in comparison with Expert 1 and Expert 2, respectively. Using retinal layer segmentation improved the model performance. The proposed model identified RORA with performance matching human experts. It has a potential to rapidly identify atrophy with high consistency.
The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
Authors: Janik-Olchawa Natalia , Drozdz Agnieszka , Ryszawy Damian , Pudelek Maciej , Planeta Karolina , Setkowicz Zuzanna , Sniegocki Maciej , Wytrwal-Sarna Magdalena , Gajewska Marta , Chwiej Joanna
Published: November 2021

Although the key factor affecting the biocompatibility of IONPs is the core size, there is a lack of regular investigation concerning the impact of the parameter on the toxicity of these nanomaterials. Therefore, such studies were carried out in this paper. Their purpose was to compare the influence of PEG-coated-magnetite NPs with the core of 5, 10 and 30 nm on six carefully selected cell lines. The proliferation rate, viability, metabolic activity, migration activity, ROS levels and cytoskeleton architecture of cells have been evaluated for specified incubation periods. These were 24 and 72-h long incubations with IONPs administered in two doses: 5 and 25 µg Fe/ml. A decrease in viability was observed after exposure to the tested NPs for all the analyzed cell lines. This effect was not connected with core diameter but depended on the exposure time to the nanomaterials. IONPs increased not only the proliferation rate of macrophages—being phagocytic cells—but also, under certain conditions stimulated tumor cell divisions. Most likely, the increase in proliferation rate of macrophages contributed to the changes in the architecture of their cytoskeleton. The growth in the level of ROS in cells had been induced mainly by the smallest NPs. This effect was observed for HEK293T cells and two cancerous lines: U87MG (at both doses tested) and T98G (only for the higher dose). This requires further study concerning both potential toxicity of such IONPs to the kidneys and assessing their therapeutic potential in the treatment of glioblastoma multiforme.
Faecal microbiota transplantation from patients with depression or healthy individuals into rats modulates mood-related behaviour
Authors: Knudsen Julie Kristine , Michaelsen Thomas Yssing , Bundgaard-Nielsen Caspar , Nielsen René Ernst , Hjerrild Simon , Leutscher Peter , Wegener Gregers , Sørensen Suzette
Published: November 2021

Differences in gut microbiota composition have been observed in patients with major depressive disorder (MDD) compared to healthy individuals. Here, we investigated if faecal microbiota transplantation (FMT) from patients with MDD into rats could induce a depressive-like phenotype. We performed FMT from patients with MDD (FMT-MDD) and healthy individuals (FMT-Healthy) into male Flinders Sensitive Line (FSL) and Flinders Resistant Line (FRL) rats and assessed depressive-like behaviour. No behavioural differences were observed in the FSL rats. In FRL rats, the FMT-Healthy group displayed significantly less depressive-like behaviour than the FMT-MDD group. However, there was no difference in behaviour between FMT-MDD FRL rats and negative controls, indicating that FMT-Healthy FRL rats received beneficial bacteria. We additionally found different taxa between the FMT-MDD and the FMT-Healthy FRL rats, which could be traced to the donors. Four taxa, three belonging to the family Ruminococcaceaeand the genus Lachnospira, were significantly elevated in relative abundance in FMT-MDD rats, while the genus Coprococcuswas depleted. In this study, the FMT-MDD group was different from the FMT-Healthy group based on behaviour and intestinal taxa.
Superfluid density, Josephson relation and pairing fluctuations in a multi-component fermion superfluid
Authors: Zhang Yi-Cai
Published: November 2021

In this work, a Josephson relation is generalized to a multi-component fermion superfluid. Superfluid density is expressed through a two-particle Green function for pairing states. When the system has only one gapless collective excitation mode, the Josephson relation is simplified, which is given in terms of the superfluid order parameters and the trace of two-particle normal Green function. In addition, it is found that the matrix elements of two-particle Green function is directly related to the matrix elements of the pairing fluctuations of superfluid order parameters. Furthermore, in the presence of inversion symmetry, the superfluid density is given in terms of the pairing fluctuation matrix. The results of the superfluid density in Haldane model show that the generalized Josephson relation can be also applied to a multi-band fermion superfluid in lattice.
The effects of bathing in neutral bicarbonate ion water
Authors: Yamazaki Tomoe , Ushikoshi-Nakayama Ryoko , Shakya Supriya , Omagari Daisuke , Matsumoto Naoyuki , Nukuzuma Chiyoko , Komatsu Tomoko , Lee Masaichi Chang-il , Inoue Hiroko , Saito Ichiro
Published: November 2021

Percutaneously absorbed carbon dioxide enhances blood flow. The mechanism by which it does so is unclear, but we hypothesized that it involves bicarbonate ions. BALB/c mice were bathed in neutral bicarbonate ionized water (NBIW) and showed increased blood bicarbonate levels and blood flow via phosphorylation of peripheral vascular endothelial nitric oxide synthase (eNOS) and production of nitric oxide (NO). Phosphorylation of eNOS and NO production were also increased in human umbilical vein endothelial cells cultured in medium containing NBIW, and NBIW showed reactive oxygen species scavenging activity. In a double-blind, randomized study in men and women aged 30 to 59 years with subjective cold intolerance, bathing in NBIW elevated body temperature faster than bathing in a control solution and improved chills and sleep quality. Taken together, our results show that percutaneously absorbed carbon dioxide changes to bicarbonate ions, which act directly on endothelial cells to increase NO production by phosphorylation of eNOS and thus improve blood flow.
Microwave power penetration enhancement inside an inhomogeneous human head
Authors: Rokunuzzaman Md. , Ahmed Asif , Baum Thomas , Rowe Wayne S. T.
Published: November 2021

The penetration of microwave power inside a human head model is improved by employing a dielectric loaded rectangular waveguide as the transmission source. A multi-layer reflection model is investigated to evaluate the combined material characteristics of different lossy human head tissues at 2.45 GHz. A waveguide loaded with a calculated permittivity of 3.62 is shown to maximise the microwave power penetration at the desired frequency. A Quartz (SiO2) loaded rectangular waveguide fed by a microstrip antenna is designed to validate the power penetration improvement inside an inhomogeneous human head phantom. A measured 1.33 dB power penetration increment is observed for the dielectric loaded waveguide over a standard rectangular waveguide at 50 mm inside the head, with an 81.9% reduction in the size of the transmission source.
Frequency and patterns of exposure to live poultry and the potential risk of avian influenza transmission to humans in urban Bangladesh
Authors: Berry Isha , Rahman Mahbubur , Flora Meerjady Sabrina , Greer Amy L. , Morris Shaun K. , Khan Iqbal Ansary , Sarkar Sudipta , Naureen Tanzila , Fisman David N. , Mangtani Punam
Published: November 2021

Avian influenza is endemic in Bangladesh, where greater than 90% of poultry are marketed through live poultry markets (LPMs). We conducted a population-based cross-sectional mobile telephone survey in urban Dhaka, Bangladesh to investigate the frequency and patterns of human exposure to live poultry in LPMs and at home. Among 1047 urban residents surveyed, 74.2% (95% CI 70.9–77.2) reported exposure to live poultry in the past year, with the majority of exposure occurring on a weekly basis. While visiting LPMs was less common amongst females (40.3%, 95% CI 35.0–45.8) than males (58.9%, 95% CI 54.0–63.5), females reported greater poultry exposure through food preparation, including defeathering (13.2%, 95% CI 9.5–17.9) and eviscerating (14.8%, 95% CI 11.2–19.4) (p < 0.001). A large proportion of the urban population is frequently exposed to live poultry in a setting where avian influenza viruses are endemic in LPMs. There is thus not only ample opportunity for spillover of avian influenza infections into humans in Dhaka, Bangladesh, but also greater potential for viral reassortment which could generate novel strains with pandemic potential.
Development of a rat capnoperitoneum phantom to study drug aerosol deposition in the context of anticancer research on peritoneal carcinomatosis
Authors: Göhler Daniel , Geldner Antje , Gritzki Ralf , Lohse Franz , Große Stephan , Sobilo Julien , Felsmann Clemens , Buggisch Jonathan R. , Le Pape Alain , Rudolph Andreas , Stintz Michael , Giger-Pabst Urs
Published: November 2021

Pressurized Intraperitoneal Aerosol Chemotherapy (PIPAC) is a promising approach with a high optimization potential for the treatment of peritoneal carcinomatosis. To study the efficacy of PIPAC and drugs, first rodent cancer models were developed. But inefficient drug aerosol supply and knowledge gaps concerning spatial drug distribution can limit the results based on such models. To study drug aerosol supply/deposition, computed tomography scans of a rat capnoperitoneum were used to deduce a virtual and a physical phantom of the rat capnoperitoneum (RCP). RCP qualification was performed for a specific PIPAC method, where the capnoperitoneum is continuously purged by the drug aerosol. In this context, also in-silico analyses by computational fluid dynamic modelling were conducted on the virtual RCP. The physical RCP was used for ex-vivo granulometric analyses concerning drug deposition. Results of RCP qualification show that aerosol deposition in a continuous purged rat capnoperitoneum depends strongly on the position of the inlet and outlet port. Moreover, it could be shown that the droplet size and charge condition of the drug aerosol define the deposition efficiency. In summary, the developed virtual and physical RCP enables detailed in-silico and ex-vivo analyses on drug supply/deposition in rodents.
Trends and disparities in osteoarthritis prevalence among US adults, 2005–2018
Authors: Xu Yingke , Wu Qing
Published: November 2021

Studies reporting trends and disparities of osteoarthritis (OA) in the United States are limited. We aimed to examine trends and disparities of OA prevalence among US adults, from 2005 to 2018. Continuous National Health and Nutrition Examination Survey (NHANES) data from 2005–2006 to 2017–2018 were analyzed. Age-adjusted and self-reported OA prevalence, stratified by race/ethnicity and socioeconomic status (SES), was calculated separately for men and women. The linear trend and the association between the survey cycles and OA prevalence were assessed. Age-adjusted and self-reported OA prevalence linearly increased in the seven survey cycles (both Plinear trend ≤ 0.0002) in men and women. Non-Hispanic Caucasians (both Plinear trend ≤ 0.0001) in both genders and Non-Hispanic African Americans women (Plinear trend ≤ 0.0001) had significantly increasing linear trends in OA prevalence. In addition, people with lower SES had a lower age-adjusted prevalence of self-reported OA when compared to those with higher SES. The increasing linear trends still existed among both men and women after adjusting for multiple confounders (both Plinear trend ≤ 0.002). There were significant rising trends and disparities in self-reported OA prevalence among US men and women between 2005 and 2018.
The hypertriglyceridemic-waist phenotype as a valuable and integrative mirror of metabolic syndrome traits
Authors: de Cuevillas Begoña , Alvarez-Alvarez Ismael , Riezu-Boj Jose I. , Navas-Carretero Santiago , Martinez J. Alfredo
Published: November 2021

Rates of non-communicable diseases (NCDs), such as obesity, diabetes, cardiovascular events and cancer, continue to rise worldwide, which require objective instruments for preventive and management actions. Diverse anthropometric and biochemical markers have been used to qualitatively evaluate degrees of disease, metabolic traits and evolution of nutritional status. The aim of this study was to integrate and assess the interactions between an anthropometric measurement, such as waist circumference (WC), and biochemical data, such as the triglyceride glucose index (TyG), in order to individually characterize metabolic syndrome (MetS) features considering the hypertriglyceridemic waist phenotype as a marker. An ancillary cross-sectional study was conducted using anthropometric measurements, such as weight, height, waist and hip circumferences, as well as fasting biochemical data of 314 participants. Different indices based on WC (WC, WC*TG and WC*TyG) were estimated to compute MetS components and accompanying comorbidities. ROC curves were fitted to define the strength of the analyses and the validity of the relationships. Associations were confirmed between anthropometric, biochemical and combined indices with some chronic disease manifestations, including hyperglycemia, hypertension and dyslipidemia. Both WC*TG and WC*TyG indices showed similar performance in diagnosing MetS (area under the ROC curve = 0.81). Interestingly, when participants were categorized according to a reference value of the WC*TyG index (842.7 cm*mg/dl), our results evidenced that subjects classified over this limit presented statistically higher prevalence of MetS and accompanying individual components with clinical relevance for interventions. These results revealed that WC*TyG mirrors the hypertriglyceridemic phenotype, which suggests may serve as a good indicator to define the metabolic syndrome phenotype and a suitable, sensitive, and simple proxy to complement others. A reference point was proposed with a good clinical performance and maximized sensitivity and specificity values.
Human endothelial cells and fibroblasts express and produce the coagulation proteins necessary for thrombin generation
Authors: Cohen Clay T. , Turner Nancy A. , Moake Joel L.
Published: November 2021

In a previous study, we reported that human endothelial cells (ECs) express and produce their own coagulation factors (F) that can activate cell surface FX without the additions of external proteins or phospholipids. We now describe experiments that detail the expression and production in ECs and fibroblasts of the clotting proteins necessary for formation of active prothrombinase (FV–FX) complexes to produce thrombin on EC and fibroblast surfaces. EC and fibroblast thrombin generation was identified by measuring: thrombin activity; thrombin–antithrombin complexes; and the prothrombin fragment 1.2 (PF1.2), which is produced by the prothrombinase cleavage of prothrombin (FII) to thrombin. In ECs, the prothrombinase complex uses surface-attached FV and γ-carboxyl-glutamate residues of FX and FII to attach to EC surfaces. FV is also on fibroblast surfaces; however, lower fibroblast expression of the gene for γ-glutamyl carboxylase (GGCX) results in production of vitamin K-dependent coagulation proteins (FII and FX) with reduced surface binding. This is evident by the minimal surface binding of PF1.2, following FII activation, of fibroblasts compared to ECs. We conclude that human ECs and fibroblasts both generate thrombin without exogenous addition of coagulation proteins or phospholipids. The two cell types assemble distinct forms of prothrombinase to generate thrombin.