bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomimetic Stratum Corneum Liposome Models: Lamellar Organization and Permeability Studies
Authors: Roy Susmita , Ho James C. S. , Teo Douglas L. C. , Gupta Shikhar , Nallani Madhavan
Published: January 2023

The stratum corneum (SC), the outer layer of the skin, plays a crucial role as a barrier protecting the underlying cells from external stress. The SC comprises three key components: ceramide (CER), free fatty acid (FFA), and cholesterol, along with small fractions of cholesterol sulfate and cholesterol ester. In order to gain a deeper understanding about the interdependence of the two major components, CER and FFA, on the organizational, structural, and functional properties of the SC layer, a library of SC lipid liposome (SCLL) models was developed by mixing CER (phytosphingosine or sphingosine), FFA (oleic acid, palmitic acid, or stearic acid), cholesterol, and cholesterol sulfate. Self-assembly of the SC lipids into lamellar phases was first confirmed by small-angle X-ray scattering. Short periodicity and long periodicity phases were identified for SCLLs containing phytosphingosines and sphingosine CERs, respectively. Furthermore, unsaturation in the CER acyl and FFA chains reduced the lipid conformational ordering and packing density of the liposomal bilayer, which were measured by differential scanning calorimetry and Fourier transform infrared spectroscopy. The introduction of unsaturation in the CER and/or FFA chains also impacted the lamellar integrity and permeability. This extensive library of SCLL models exhibiting physiologically relevant lamellar phases with defined structural and functional properties may potentially be used as a model system for screening pharmaceuticals or cosmetic agents.
Ultrafiltration of Black Soldier Fly (Hermetia illucens) and Mealworm (Tenebrio molitor) Protein Concentrates to Enhance Emulsifying and Foaming Properties
Authors: Ranasinghe Madushika K. , Ballon Aurélie , de Lamo-Castellví Sílvia , Ferrando Montserrat , Güell Carme
Published: January 2023

Mealworm, TM (Tenebrio molitor), and black soldier fly, BSF (Hermetia illucens) are of special interest for food and feed applications due to their environmental benefits such as low water and land requirements, low greenhouse gas emissions, and high feed-conversion efficiency. This study assesses the use of ultrafiltration (UF) to fractionate protein concentrates from TM and BSF (TMPC, BSFPC) in order to enhance emulsifying and foaming properties. A 30 kDa regenerated cellulose acetate membrane enabled the separation of concentrate and permeate fractions for both insect proteins from two different initial feed concentrations (10 and 7.5 g/L). Permeate flux and protein transmission behave differently depending on the insect type and the initial concentration; while for TMPC permeate flux increases with a decrease in the initial protein concentration, it is not affected for BSFPC. The existing membrane cleaning protocols are suitable for recovering water flux after UF of insect proteins, enabling membrane re-use. Emulsifying activity is maintained for all the TMPC fractions, but it is significantly lower for the permeate fractions of BSFPC. Foaming properties are maintained for all the UF fractions of BSFPC and the ones from 7.5 g/L TMPC. Acidic solubilization leads to a fraction with enhanced emulsifying capacity and one with higher foaming capacity than the original for BSFPC. This study opens the door to membrane technology for insect protein fractionation, which has not been studied so far and has already provided useful solutions for other animal and plant proteins.
A Novel Carbide-Free Bainitic Heavy-Haul Wheel Steel with an Excellent Wear-Resistance under Rolling-Sliding Condition
Authors: Zhou Tingwei , Xu Haifeng , Ma Xinyuan , Xu Zhenlin , Zhao Hai , He Yizhu
Published: January 2023

With the rapid development of railways towards high speed and larger carrying capacity, the problem of wear and fatigue damage between wheel/rail is gradually becoming serious. However, traditional pearlite wheel/rail has reached the limit, which leads to more attention to developing a novel wheel/rail material. This study aims to report a novel carbide-free bainite wheel steel. The wear-resistance of novel steel was tested by a rolling-sliding wear experiment under heavy-haul condition and investigated the impacts of the running speeds on the damage mechanism of wear and fatigue. The results show that the yield strength of the bainite wheel was as high as 950 MPa and the hardness was 415 HV, which was superior to most of the reported typical wheel steel. During the process of wear, the surface damage of the wheel was mainly adhesive wear and fatigue damage, and the gradient strain layer (GS layer) was formed on the wheel surface. As the running speed increased, fatigue damage gradually became more serious than adhesive wear, and the shear stress and strain of the GS layer were enhanced. The higher thickness and hardening were produced on the GS layer, which is the main reason for the higher wear-resistance of the bainitic wheel under higher running speeds. In addition, the wear-resistance of the novel wheel steel was better than that of the reported wheel steel. This novel bainitic wheel is a promising wheel for heavy-haul condition applications, which could provide a guide in choosing bainitic wheel steel for the railway.
Electrodeposition of Tin-Reduced Graphene Oxide Composite from Deep Eutectic Solvents Based on Choline Chloride and Ethylene Glycol
Authors: Costovici Stefania , Pantazi Aida , Balan Danut , Cojocaru Anca , Visan Teodor , Enachescu Marius , Anicai Liana
Published: January 2023

Some experimental results regarding the direct electrodeposition of tin-reduced graphene oxide composite (Sn-rGO) compared to the electrodeposition of tin metal (Sn) from a deep eutectic solvent (DES), namely using choline chloride-ethylene glycol eutectic mixtures, are presented. Raman spectroscopy demonstrated that GO is also reduced during the tin electrodeposition. Scanning electron microscopy (SEM) confirmed the presence of incorporated graphene related material in the composite film. X-ray diffraction patterns showed that the presence of rGO in the deposit diminished preferred orientation of Sn growth along the planes (101), (211), (301), and (112). The analysis of current-time transients involving Scharifker & Hills model has shown that Sn-rGO composite deposition process corresponds to a nucleation and tridimensional growth controlled by diffusion, with nucleation evolving from progressive to instantaneous upon increasing the overpotential. Diffusion coefficients at 25 °C of 9.4 × 10−7 cm2 s−1 for Sn(II) species in the absence and of 14.1 × 10−7 cm2 s−1 in the presence of GO, were determined. The corrosion performance has been assessed through the analysis of the recorded potentiodynamic polarization curves and of the electrochemical impedance spectra during continuous immersion in aerated 0.5 M NaCl aqueous solution at 25 °C for 144 h. A slight improvement of the corrosion performance in the case of the Sn-rGO composite coatings was noticed, as compared to pure Sn ones. Furthermore, the solderability performance has been evaluated. The solder joints showed a proper adhesion to the substrate with no fractures, and wetting angles around 44° have been determined, suggesting adequate solderability characteristics.
Microscale Strain Localizations and Strain-Induced Martensitic Phase Transformation in Austenitic Steel 301LN at Different Strain Rates
Authors: Pun Lalit , Soares Guilherme Corrêa , Bhusare Suprit , Isakov Matti , Hokka Mikko
Published: January 2023

Microscopic strain and strain-induced phase transformation during plastic deformation in metastable austenitic steel were investigated at different strain rates. Quasi in-situ tension tests were performed sequentially with well-defined elongation intervals at room temperature at strain rates of 10−3 s−1 and 10−1 s−1. The tests were monitored by high-resolution optical imaging with a microscopic lens at a resolution of 0.23 µm/pixel. The macroscopic temperature was also measured with an infrared (IR) camera. The microstructure-level strain localizations were observed on the surface of the etched specimens by means of microscale digital image correlation (µDIC). Additionally, the microstructure was characterized by electron backscatter diffraction (EBSD) at the same location before and after deformation. The results of the study indicated that microscopic strain localizations favored the formation of α′-martensite particles. At the lower strain rate, high local strain concentrations formed at several locations in the microstructure, correlating with the areas where the formation of large martensite islands occurred. Martensite particles of various sizes formed nearby each other at the lower strain rate, whereas at the higher strain rate, martensite islands remained small and isolated. Although the macroscopic increase in temperature at both the studied strain rates was very low, at the higher strain rate, local heating on the microscopic scale could take place at the newly nucleated martensite embryos. This inhibited the further growth of the martensite particles, and local strain distribution also remained more homogeneous than at the lower strain rate.
Meibomian Gland Assessment in Routine Ophthalmology Practice
Authors: Petriček Igor , Tomić Martina , Bulum Tomislav , Lešin Gaćina Dina , Vidas Pauk Sania
Published: January 2023

This cross-sectional study aimed to investigate the connection between meibomian gland (MG) excreta quantity and quality after MG expression (MGX), dry eye disease (DED) symptoms, and objective DED signs and to clarify the relationship between dry eye and MG function in DED pathophysiology. The study included 200 subjects, 100 with and 100 without dry eye symptoms. Schein questionnaire was used to determine the severity of dry eye symptoms and self-reported skin type for facial skin dryness self-evaluation. Objective dry eye signs were assessed by monitoring conjunctival hyperemia, lid parallel conjunctival folds (LIPCOF), tear break-up time (TBUT), fluorescein surface staining and digital MGX. Subjects with DED symptoms had significantly lower MG quantity scores than healthy controls (p < 0.001). Meibum quality and quantity scores significantly correlated with female gender (p = 0.002), Schein questionnaire score (p < 0.001), fluorescein corneal staining score (p = 0.019), self-reported skin type (p < 0.001), TBUT (p < 0.001) and LIPCOF (p = 0.041). After adjustment for age and gender in a logistic regression analysis, dry eye was independently and significantly associated with self-reported skin type (OR 0.73, p < 0.001), LIPCOF (OR 1.04, p < 0.001), fluorescein corneal staining (OR 1.05, p = 0.019), TBUT (OR 0.77, p < 0.001) and meibum quantity score (OR 0.59, p < 0.001). Dry eye symptoms and objective signs correlated well in this study. MGX discriminated between the subjects with and without DED symptoms and was associated with other objective DED signs. Results showed a significant association between meibum quality and quantity, MG function, DED and facial skin dryness self-perception. This paper established a correlation between dry eye symptoms caused by MG dysfunction and dry skin, which can help general health practitioners consider dry eye as a cause of chronic eye complaints with patients who report dry skin.
A Nitrate-Transforming Bacterial Community Dominates in the Miscanthus Rhizosphere on Nitrogen-Deficient Volcanic Deposits of Miyake-jima
Authors: Arsyadi Ahmad , Guo Yong , Ebihara Akiko , Sakagami Nobuo , Sakoda Midori , Tago Kanako , Kamijo Takashi , Ohta Hiroyuki , Nishizawa Tomoyasu
Published: January 2023

The perennial gramineous grass Miscanthus condensatus functions as a major pioneer plant in colonizing acidic volcanic deposits on Miyake-jima, Japan, despite a lack of nitrogen nutrients. The nitrogen cycle in the rhizosphere is important for the vigorous growth of M. condensatus in this unfavorable environment. In the present study, we identified the nitrogen-cycling bacterial community in the M. condensatus rhizosphere on these volcanic deposits using a combination of metagenomics and culture-based analyses. Our results showed a large number of functional genes related to denitrification and dissimilatory nitrate reduction to ammonium (DNRA) in the rhizosphere, indicating that nitrate-transforming bacteria dominated the rhizosphere biome. Furthermore, nitrite reductase genes (i.e., nirK and nirS) related to the denitrification and those genes related to DNRA (i.e., nirB and nrfA) were mainly annotated to the classes Alpha-proteobacteria, Beta-proteobacteria, and Gamma-proteobacteria. A total of 304 nitrate-succinate-stimulated isolates were obtained from the M. condensatus rhizosphere and were classified into 34 operational taxonomic units according to amplified 16S rRNA gene restriction fragment pattern analysis. Additionally, two strains belonging to the genus Cupriavidus in the class Beta-proteobacteria showed a high in vitro denitrifying activity; however, metagenomic results indicated that the DNRA-related rhizobacteria appeared to take a major role in the nitrogen cycle of the M. condensatus rhizosphere in recent Miyake-jima volcanic deposits. This study elucidates the association between the Miscanthus rhizosphere and the nitrate-reducing bacterial community on newly placed volcanic deposits, which furthers our understanding of the transformation of nitrogen nutrition involved in the early development of vegetation.
Endotoxemia and Gastrointestinal Cancers: Insight into the Mechanisms Underlying a Dangerous Relationship
Authors: Manilla Vittoria , Di Tommaso Natalia , Santopaolo Francesco , Gasbarrini Antonio , Ponziani Francesca Romana
Published: January 2023

Lipopolysaccharide (LPS), also known as endotoxin, is a component of the membrane of gram-negative bacteria and a well-recognized marker of sepsis. In case of disruption of the intestinal barrier, as occurs with unhealthy diets, alcohol consumption, or during chronic diseases, the microbiota residing in the gastrointestinal tract becomes a crucial factor in amplifying the systemic inflammatory response. Indeed, the translocation of LPS into the bloodstream and its interaction with toll-like receptors (TLRs) triggers molecular pathways involved in cytokine release and immune dysregulation. This is a critical step in the exacerbation of many diseases, including metabolic disorders and cancer. Indeed, the role of LPS in cancer development is widely recognized, and examples include gastric tumor related to Helicobacter pylori infection and hepatocellular carcinoma, both of which are preceded by a prolonged inflammatory injury; in addition, the risk of recurrence and development of metastasis appears to be associated with endotoxemia. Here, we review the mechanisms that link the promotion and progression of tumorigenesis with endotoxemia, and the possible therapeutic interventions that can be deployed to counteract these events.
Development of Efficient Genome-Reduction Tool Based on Cre/loxP System in Rhodococcus erythropolis
Authors: Kitagawa Wataru , Hata Miyako
Published: January 2023

Rhodococcus has been extensively studied for its excellent ability to degrade artificial chemicals and its capability to synthesize biosurfactants and antibiotics. In recent years, studies have attempted to use Rhodococcus as a gene expression host. Various genetic tools, such as plasmid vectors, transposon mutagenesis, and gene disruption methods have been developed for use in Rhodococcus; however, no effective method has been reported for performing large-size genome reduction. Therefore, the present study developed an effective plasmid-curing method using the levansucrase-encoding sacB gene and a simple two-step genome-reduction method using a modified Cre/loxP system. For the results, R. erythropolis JCM 2895 was used as the model; a mutant strain that cured all four plasmids and deleted seven chromosomal regions was successfully obtained in this study. The total DNA deletion size was >600 kb, which corresponds mostly to 10% of the genome size. Using this method, a genome-structure-stabilized and unfavorable gene/function-lacking host strain can be created in Rhodococcus. This genetic tool will help develop and improve Rhodococcus strains for various industrial and environmental applications.
Responses of Free-Living Planktonic Bacterial Communities to Experimental Acidification and Warming
Authors: Tsiola Anastasia , Krasakopoulou Evangelia , Daffonchio Daniele , Frangoulis Constantin , Tsagaraki Tatiana M. , Fodelianakis Stilianos , Pitta Paraskevi
Published: January 2023

Climate change driven by human activities encompasses the increase in atmospheric CO2 concentration and sea-surface temperature. Little is known regarding the synergistic effects of these phenomena on bacterial communities in oligotrophic marine ecosystems that are expected to be particularly vulnerable. Here, we studied bacterial community composition changes based on 16S rRNA sequencing at two fractions (0.1–0.2 and >0.2 μm) during a 10- day fully factorial mesocosm experiment in the eastern Mediterranean where the pH decreased by ~0.3 units and temperature increased by ~3 °C to project possible future changes in surface waters. The bacterial community experienced significant taxonomic differences driven by the combined effect of time and treatment; a community shift one day after the manipulations was noticed, followed by a similar state between all mesocosms at the third day, and mild shifts later on, which were remarkable mainly under sole acidification. The abundance of Synechococcus increased in response to warming, while the SAR11 clade immediately benefited from the combined acidification and warming. The effect of the acidification itself had a more persistent impact on community composition. This study highlights the importance of studying climate change consequences on ecosystem functioning both separately and simultaneously, considering the ambient environmental parameters.
Partitioning and Migration of Trace Elements during Coal Combustion in Two Coal-Fired Power Plants in Hefei City, Anhui Province, Eastern China
Authors: Jiang Peijun , Chen Jian , Li Yang , Li Xiuli , Qi Xiaowei , Wang Jiayi , Chen Ping , Liu Wenzhong , Wang Ruwei
Published: January 2023

Coal-fired thermal power plants that meet the huge energy demand of China largely contribute to carbon emissions, environmental pollution, and human health issues. To investigate the impact of coal-fired power plants on the environment in the most developed region of eastern China, samples of feed coal, limestone, slag, fly ash, and flue gas desulfurization (FGD) gypsum were collected from two coal-fired power plants in Hefei City, Anhui Province, China. The concentrations of trace elements in these samples were determined using inductively coupled plasma mass spectrometry (ICP-MS). The results show that the feed coal was slightly rich in V, Cr, Se, In, Sb, Pb, and Ga compared to common Chinese coal. Most trace elements were concentrated in fly ash, especially in the fine particles. Only Sc, Cr, Rb, and Ba were enriched in slag. The smaller the particle size of the fly ash, the higher the fraction of volatile trace elements. The elemental concentrations of old fly ash, which was precipitated in the well of the flue gas pipeline, did not show noticeable differences from fresh fly ash. In addition, the short-time storage of fly ash did not influence the trace elements. To understand the possibility of recovering rare earth elements from coal combustion by-products, the outlook coefficients (Coutl) of coal combustion by-products for the Wanneng (WN) and Tianyuan (TY) power plants were calculated, and they were found to be promising for rare earth elements plus yttrium (REY) recovery. However, as per the evaluation diagram of cut-off grade and Coutl values, all feed coal and its by-products from these two coal-fired power plants are projected to be ‘unpromising’. Therefore, the coal combustion by-products from the WN and TY coal-fired power plants were not candidates for REY recovery.
Smiles and Angry Faces vs. Nods and Head Shakes: Facial Expressions at the Service of Autonomous Vehicles
Authors: Rouchitsas Alexandros , Alm Håkan
Published: January 2023

When deciding whether to cross the street or not, pedestrians take into consideration information provided by both vehicle kinematics and the driver of an approaching vehicle. It will not be long, however, before drivers of autonomous vehicles (AVs) will be unable to communicate their intention to pedestrians, as they will be engaged in activities unrelated to driving. External human–machine interfaces (eHMIs) have been developed to fill the communication gap that will result by offering information to pedestrians about the situational awareness and intention of an AV. Several anthropomorphic eHMI concepts have employed facial expressions to communicate vehicle intention. The aim of the present study was to evaluate the efficiency of emotional (smile; angry expression) and conversational (nod; head shake) facial expressions in communicating vehicle intention (yielding; non-yielding). Participants completed a crossing intention task where they were tasked with deciding appropriately whether to cross the street or not. Emotional expressions communicated vehicle intention more efficiently than conversational expressions, as evidenced by the lower latency in the emotional expression condition compared to the conversational expression condition. The implications of our findings for the development of anthropomorphic eHMIs that employ facial expressions to communicate vehicle intention are discussed.
Irreversibility Marangoni Tri-Hybrid Nanoflow Analysis for Thermal Enhancement Applications
Authors: Ullah Malik Zaka
Published: January 2023

Increasing heat transfer is an important part of industrial, mechanical, electrical, thermal, and biological sciences. The aim of this study is to increase the thermal competency of a conventional fluid by using a ternary hybrid nanofluid. A magnetic field and thermal radiation are used to further improve the thermal conductivity of the base fluid. Irreversibility is analyzed under the influence of the embedded parameters. The basic equations for the ternary hybrid nanofluids are transformed from Partial Differential Equations (PDEs) to Ordinary Differential Equations (ODEs) using the similarity concept. The Marangoni convection idea is used in the mathematical model for the temperature difference between the two media: the surface and fluid. The achieved results are provided and discussed. The results show that ternary hybrid nanofluids are more suitable as heat-transmitted conductors than conventional fluids.
Biogenic Selenium Nanoparticles in Biomedical Sciences: Properties, Current Trends, Novel Opportunities and Emerging Challenges in Theranostic Nanomedicine
Authors: Zambonino Marjorie C. , Quizhpe Ernesto Mateo , Mouheb Lynda , Rahman Ashiqur , Agathos Spiros N. , Dahoumane Si Amar
Published: January 2023

Selenium is an important dietary supplement and an essential trace element incorporated into selenoproteins with growth-modulating properties and cytotoxic mechanisms of action. However, different compounds of selenium usually possess a narrow nutritional or therapeutic window with a low degree of absorption and delicate safety margins, depending on the dose and the chemical form in which they are provided to the organism. Hence, selenium nanoparticles (SeNPs) are emerging as a novel therapeutic and diagnostic platform with decreased toxicity and the capacity to enhance the biological properties of Se-based compounds. Consistent with the exciting possibilities offered by nanotechnology in the diagnosis, treatment, and prevention of diseases, SeNPs are useful tools in current biomedical research with exceptional benefits as potential therapeutics, with enhanced bioavailability, improved targeting, and effectiveness against oxidative stress and inflammation-mediated disorders. In view of the need for developing eco-friendly, inexpensive, simple, and high-throughput biomedical agents that can also ally with theranostic purposes and exhibit negligible side effects, biogenic SeNPs are receiving special attention. The present manuscript aims to be a reference in its kind by providing the readership with a thorough and comprehensive review that emphasizes the current, yet expanding, possibilities offered by biogenic SeNPs in the biomedical field and the promise they hold among selenium-derived products to, eventually, elicit future developments. First, the present review recalls the physiological importance of selenium as an oligo-element and introduces the unique biological, physicochemical, optoelectronic, and catalytic properties of Se nanomaterials. Then, it addresses the significance of nanosizing on pharmacological activity (pharmacokinetics and pharmacodynamics) and cellular interactions of SeNPs. Importantly, it discusses in detail the role of biosynthesized SeNPs as innovative theranostic agents for personalized nanomedicine-based therapies. Finally, this review explores the role of biogenic SeNPs in the ongoing context of the SARS-CoV-2 pandemic and presents key prospects in translational nanomedicine.
Catalyst Design: Counter Anion Effect on Ni Nanocatalysts Anchored on Hollow Carbon Spheres
Authors: O’Connor Ryan , Matsoso Joyce B. , Mashindi Victor , Mente Pumza , Macheli Lebohang , Moreno Beatriz D. , Doyle Bryan P. , Coville Neil J. , Barrett Dean H.
Published: January 2023

Herein, the influence of the counter anion on the structural properties of hollow carbon spheres (HCS) support was investigated by varying the nickel metal precursor salts applied. TEM and SEM micrographs revealed the dimensional dependence of the HCS shell on the Ni precursor salt, as evidenced by thick (~42 nm) and thin (~23 nm) shells for the acetate and chloride-based salts, respectively. Importantly, the effect of the precursor salt on the textural properties of the HCS nanosupports (~565 m2/gNi(acet)) and ~607 m2/gNiCl), influenced the growth of the Ni nanoparticles, viz for the acetate-(ca 6.4 nm)- and chloride (ca 12 nm)-based salts, respectively. Further, XRD and PDF analysis showed the dependence of the reduction mechanism relating to nickel and the interaction of the nickel–carbon support on the type of counter anion used. Despite the well-known significance of the counter anion on the size and crystallinity of Ni nanoparticles, little is known about the influence of such counter anions on the physicochemical properties of the carbon support. Through this study, we highlight the importance of the choice of the Ni-salt on the size of Ni in Ni–carbon-based nanocatalysts.
Controlled Reduction of Sn4+ in the Complex Iodide Cs2SnI6 with Metallic Gallium
Authors: Umedov Shodruz T. , Grigorieva Anastasia V. , Sobolev Alexey V. , Knotko Alexander V. , Lepnev Leonid S. , Kolesnikov Efim A. , Charkin Dmitri O. , Shevelkov Andrei V.
Published: January 2023

Metal gallium as a low-melting solid was applied in a mixture with elemental iodine to substitute tin(IV) in a promising light-harvesting phase of Cs2SnI6 by a reactive sintering method. The reducing power of gallium was applied to influence the optoelectronic properties of the Cs2SnI6 phase via partial reduction of tin(IV) and, very likely, substitute partially Sn4+ by Ga3+. The reduction of Sn4+ to Sn2+ in the Cs2SnI6 phase contributes to the switching from p-type conductivity to n-type, thereby improving the total concentration and mobility of negative-charge carriers. The phase composition of the samples obtained was studied by X-ray diffraction (XRD) and 119Sn Mössbauer spectroscopy (MS). It is shown that the excess of metal gallium in a reaction melt leads to the two-phase product containing Cs2SnI6 with Sn4+ and β-CsSnI3 with Sn2+. UV–visible absorption spectroscopy shows a high absorption coefficient of the composite material.
The Alignment of Recommendations of Dietary Guidelines with Sustainability Aspects: Lessons Learned from Italy’s Example and Proposals for Future Development
Authors: Rossi Laura , Ferrari Marika , Ghiselli Andrea
Published: January 2023

The main objective of this paper is to describe the process of the inclusion of sustainability in the Italian Dietary Guidelines (IDGs). In the IDGs’ sustainability chapter, particular emphasis was put on the selection of foods, recommending a plant-based diet with a large quota of vegetable proteins. Advice was also given on the selection of local seasonal products, with low growth input, such as fertilizers, artificial light and heating. Reduction of animal food was recommended, to be substituted with lower impact foods (poultry, milk, eggs and Mediterranean fish including aquaculture). Food waste was largely addressed. Recommendations were made for food purchase planning, food storage and the reuse of leftovers as strategies to reduce waste and save money. The IDGs sustainability recommendations were related to the 16 guiding principles of a sustainable healthy diet and their contribution to the achievement of the Sustainable Developing Goals was provided. The inclusion of sustainability in dietary guidelines requires a multidisciplinary approach to cover the wide range of aspects of a sustainable diet. In the IDGs, it was possible to show that practical recommendations for improving sustainability behavior can be passed on to consumers. Methodological aspects for developing recommendations are not definitive.
Protein Ingestion in Reducing the Risk of Late-Onset Post-Exercise Hypoglycemia: A Pilot Study in Adolescents and Youth with Type 1 Diabetes
Authors: Paramalingam Nirubasini , Keating Barbara L. , Chetty Tarini , Fournier Paul A. , Soon Wayne H. K. , O’Dea Joanne M. , Roberts Alison G. , Horowitz Michael , Jones Timothy W. , Davis Elizabeth A.
Published: January 2023

Dietary protein causes dose-dependent hyperglycemia in individuals with type 1 diabetes (T1D). This study investigated the effect of consuming 50 g of protein on overnight blood glucose levels (BGLs) following late-afternoon moderate-intensity exercise. Six participants (3M:3F) with T1D, HbA1c 7.5 ± 0.8% (58.0 ± 8.7 mmol/mol) and aged 20.2 ± 3.1 years exercised for 45 min at 1600 h and consumed a protein drink or water alone at 2000 h, on two separate days. A basal insulin euglycemic clamp was employed to measure the mean glucose infusion rates (m-GIR) required to maintain euglycemia on both nights. The m-GIR on the protein and water nights during the hypoglycemia risk period and overnight were 0.27 ± 043 vs. 1.60 ± 0.66 mg/kg/min (p = 0.028, r = 0.63) and 0.51 ± 0.16 vs. 1.34 ± 0.71 mg/kg/min (p = 0.028, r = 0.63), respectively. Despite ceasing intravenous glucose infusion on the protein night, the BGLs peaked at 9.6 ± 1.6 mmol/L, with a hypoglycemia risk period mean of 7.8 ± 1.5 mmol/L compared to 5.9 ± 0.4 mmol/L (p = 0.028) on the water night. The mean plasma glucagon levels were 51.5 ± 14.1 and 27.2 ± 10.1 ng/L (p = 0.028) on the protein and water night, respectively. This suggests that an intake of protein is effective at reducing the post-exercise hypoglycemia risk, potentially via a glucagon-mediated stimulation of glucose production. However, 50 g of protein may be excessive for maintaining euglycemia.
Leprosy in an Adopted Woman Diagnosed by Molecular Tools: A Case Report from a Non-Endemic Area
Authors: Beltrame Anna , Fargnoli Maria Concetta , Avanzi Charlotte , Sollima Laura , Pomari Elena , Mori Antonio , Longoni Silvia Stefania , Moro Lucia , Orza Pierantonio , Jackson Mary , Perandin Francesca
Published: January 2023

Coupled with its rarity in non-endemic areas, the clinical heterogeneity of leprosy makes diagnosis very challenging. We report a diagnosis of multibacillary leprosy in a 22-year-old Indian woman, adopted at the age of 10 and living in Italy. The patient presented with painful skin lesions on the face, trunk, and lower and upper extremities, associated with dysesthesia and a motor deficit in her left leg following corticosteroid therapy interruption. Histopathology results from the skin lesions suggested leprosy, but no acid-fast bacilli were identified. Molecular biology in a center specializing in tropical diseases confirmed the diagnosis, allowing prompt and adequate treatment. Genotype analysis allowed the identification of a genotype 1D of M. leprae, facilitating the epidemiological investigation of the plausible infection origin. No resistances to rifampicin, dapsone, or ofloxacin were detected. Leprosy will continue to exist in high-income nations, and the incidence may rise over time due to increasing migration and globalization. CARE guidelines were followed.
Design of Functional RGD Peptide-Based Biomaterials for Tissue Engineering
Authors: Kumar Vijay Bhooshan , Tiwari Om Shanker , Finkelstein-Zuta Gal , Rencus-Lazar Sigal , Gazit Ehud
Published: January 2023

Tissue engineering (TE) is a rapidly expanding field aimed at restoring or replacing damaged tissues. In spite of significant advancements, the implementation of TE technologies requires the development of novel, highly biocompatible three-dimensional tissue structures. In this regard, the use of peptide self-assembly is an effective method for developing various tissue structures and surface functionalities. Specifically, the arginine–glycine–aspartic acid (RGD) family of peptides is known to be the most prominent ligand for extracellular integrin receptors. Due to their specific expression patterns in various human tissues and their tight association with various pathophysiological conditions, RGD peptides are suitable targets for tissue regeneration and treatment as well as organ replacement. Therefore, RGD-based ligands have been widely used in biomedical research. This review article summarizes the progress made in the application of RGD for tissue and organ development. Furthermore, we examine the effect of RGD peptide structure and sequence on the efficacy of TE in clinical and preclinical studies. Additionally, we outline the recent advancement in the use of RGD functionalized biomaterials for the regeneration of various tissues, including corneal repair, artificial neovascularization, and bone TE.
Broadband Microwave Photonic Channelizer with 18 Channels Based on Acousto-Optic Frequency Shifter
Authors: Chen Bo , Dong Qunfeng , Cao Biao , Zhai Weile , Gao Yongsheng
Published: January 2023

A microwave photonic channelizer can achieve instantaneous reception of ultra-wideband signals and effectively avoid electronic bottleneck; therefore, it can be perfectly applied to a wideband radar system and electronic warfare. In channelization schemes based on an optical frequency comb (OFC), the number of comb lines usually depends on that of the sub-channels. In order to improve the utilization rate of the comb lines of OFC, we propose a scheme to shift the frequency of OFC by using an acousto-optic frequency shifter (AOFS), which can obtain three times the number of sub-channels of the comb lines of an OFC. In order to simplify the experiment, only a three-line OFC is used in the experiment. A three-line local oscillator (LO) OFC is frequency-shifted up and down by two AOFSs, and nine optical LO signals with different frequencies are obtained, thereby realizing the simultaneous reception of eighteen sub-channels. The proposed scheme enjoys a large number of sub-channels and minimal channel crosstalk. Experimental results demonstrate that a 9-GHz bandwidth RF signal covering 10–19 GHz is divided into 18 sub-channels with a sub-bandwidth of 500 MHz. The image rejection ratio of the sub-channels is about 23 dB, and the spurious-free dynamic range (SFDR) of the receiver can reach 98 dB·Hz2/3.
Response of Cd, Zn Translocation and Distribution to Organic Acids Heterogeneity in Brassica juncea L.
Authors: Liao Yumeng , Li Zuran , Yang Zhichen , Wang Jixiu , Li Bo , Zu Yanqun
Published: January 2023

In order to investigate the translocation, distribution, and organic acid heterogeneity characteristics in Brassica juncea L., a pot experiment with the exogenous application of Cd and Zn was conducted to analyze the effects of Cd, Zn, and organic acid contents and heterogeneity on the translocation and distribution of Cd and Zn. The results showed that the Cd and Zn contents of B. juncea were mainly accumulated in the roots. The Cd content in the symplast sap was 127.66–146.50% higher than that in the apoplast sap, while the opposite was true for Zn. The distribution of Cd in xylem sap occupied 64.60% under 20 mg kg−1 Cd treatment, and Zn in xylem sap occupied 60.14% under 100 mg kg−1 Zn treatment. The Cd was predominantly distributed in the vacuole, but the Zn was predominantly distributed in the cell walls. In addition, oxalic and malic acids were present in high concentrations in B. juncea. In the vacuole, correlation analysis showed that the contents of Cd were negatively correlated with the contents of oxalic acid and succinic acid, and the contents of Zn were positively correlated with the contents of malic acid and acetic acid. The contents of Cd and Zn were negatively related to the contents of oxalic acid and citric acid in xylem sap. Therefore, Cd in B. juncea was mainly absorbed through the symplast pathway, and Zn was mainly absorbed through the apoplast pathway, and then Cd and Zn were distributed in the vacuole and cell walls. The Cd and Zn in B. juncea are transferred upward through the xylem and promoted by oxalic acid, malic acid, and citric acid.
Metabolite Profiling and Bioactivities of Leaves, Stems, and Flowers of Rumex usambarensis (Dammer) Dammer, a Traditional African Medicinal Plant
Authors: Spaggiari Chiara , Righetti Laura , Spadini Costanza , Annunziato Giannamaria , Nsanzurwimo Aimable , Cabassi Clotilde Silvia , Bruni Renato , Costantino Gabriele
Published: January 2023

The comprehensive identification of secondary metabolites represents a fundamental step for the assessment of bioactivities and pharmacological properties of traditional herbal drugs. Rumex usambarensis (Dammer) Dammer has been described as a multipurpose remedy in different African traditional pharmacopoeias, but its phytochemical profile has not been properly investigated. Herein we report a high throughput metabolomic screening, based on ultra-high performance liquid chromatography-travelling wave ion mobility spectrometry quadrupole time-of-flight (UHPLC-TWINS-QTOF), which was performed for the first time on different R. usambarensis plant parts. By applying high-resolution mass spectrometry-based metabolomics and chemometric analysis, a complete discrimination of different aerial parts was obtained, with the annotation of 153 significant metabolites in leaves, stems, and flowers, suggesting an easy authentication and discrimination route. Phytochemical data were correlated to antimicrobial and antioxidant properties. Flavonoids, benzopyranes, chromones, and xanthones derivatives, along with a richer phytocomplex, might be responsible for the stronger bioactivities obtained from flowers.
Synergistic Flame Retardant Properties of Polyoxymethylene with Surface Modified Intumescent Flame Retardant and Calcium Carbonate
Authors: Yang Zheng , Chen Xueting , Lu Shike , Wang Zhenhua , Li Jiantong , Liu Baoying , Fang Xiaomin , Ding Tao , Xu Yuanqing
Published: January 2023

Ammonium polyphosphate (APP) was successfully modified by a titanate coupling agent which was compounded with benzoxazine (BOZ) and melamine (ME) to become a new type of intumescent flame retardant (Ti-IFR). Ti-IFR and CaCO3 as synergists were utilized to modify polyoxymethylene (POM), and the flame-retardant properties and mechanism of the composites were analyzed by vertical combustion (UL-94), limiting oxygen index (LOI), TG-IR, and cone calorimeter (Cone), etc. The results show that Ti-IFR can enhance the gas phase flame retardant effect, while CaCO3 further strengthens the barrier effect in the condensed phase. When they were used together, they can exert their performance, respectively, at the same time showing excellent synergistic effect. The FR-POM composite with 29% Ti-IFR and 1% CaCO3 can pass the UL-94 V0 level. The LOI reaches 58.2%, the average heat release (Av HRR) is reduced by 81.1% and the total heat release (THR) is decreased by 35.3%.
Exopolysaccharides of Fungal Origin: Properties and Pharmaceutical Applications
Authors: Stoica Roxana Mădălina , Moscovici Misu , Lakatos Elena Simina , Cioca Lucian Ionel
Published: January 2023

Fungal exopolysaccharides (EPSs) represent an important group of bioactive compounds secreted by fungi. These biopolymers can be utilized individually or in combination with different bioactive substances for a broad range of pharmaceutical field applications, due to their various biological activities, such as antioxidant, antimicrobial, anti-inflammatory, antiviral, anti-diabetic, and anticoagulant effects. The paper presents an up-to-date review of the main fungal polysaccharides (pullulan, schizophyllan, scleroglucan, botryosphaeran, lentinan, grifolan, and lasiodiplodan), highlighting their structures, producing strains, and useful properties in a double position, as controlled release (rate and selectively targeting) drug carriers, but mostly as active immunomodulating and antitumor compounds in cancer therapy.
Innovative Materials and Processes for Removal of Biopersistent Pollutants
Authors: Petrella Andrea , Race Marco , Spasiano Danilo
Published: January 2023

The aim of this Special Issue “Innovative Materials and Processes for Removal of Biopersistent Pollutants” (https://www [...]
Characterizing ‘New Korean Confucianism’: Focusing on Pak Chonghong and Yi Sang-ŭn’s Life and Thought
Authors: Gao Xing-Ai , Chung So-Yi
Published: January 2023

This article attempts to characterize some important aspects of ‘New Korean Confucianism,’ by focusing on the life and thoughts of two major thinkers of 20th century Korea, namely Pak Chonghong (1903–1976) and Yi Sang-ŭn (1905–1976). While there are volumes of studies on ‘New Confucianism’, the focus remains mostly on Chinese academics; however, just like Song–Ming, Neo-Confucianism spread throughout East Asian countries, where a unique and distinct Neo-Confucian model emerged with its own arguments and debates. The New Confucianism that appeared at the turn of the 20th century in an attempt to embrace Western cultural power within a Confucian value system has also been extended and widely adopted in Korea and has transformed itself according to its socio-political environment. Pak and Yi, who lived during and after the Japanese colonial period, struggled to grasp a sense of autonomy as the unique Korean tradition and spirit was about to be swept away by a flood of foreign ideas and social turmoil. Although the two thinkers differed in their approaches—Pak studied Western philosophy and Yi Chinese New Confucianism—they devoted their life to uncovering and systematizing the distinctive structure of Korean traditional philosophy and thereby laid the cornerstone of New Korean Confucianism.
“We Take Hold of the White Man’s Worship with One Hand, but with the Other Hand We Hold Fast Our Fathers’ Worship”: The Beginning of Indigenous Methodist Christianity and Its Expression in the Christian Guardian, Upper Canada circa 1829
Authors: Kim-Cragg David Andrew
Published: January 2023

With more and more evidence coming to light of the cultural genocide inflicted by settler Christians upon Indigenous peoples through the residential school system, it is hard to see how Christian and Indigenous identities can hold together in the current Canadian context. Nevertheless, many in the Indigenous community within Canada continue to call themselves Christian, and Indigenous Christians continue to provide important leadership for the Canadian church. This phenomenon cannot be properly understood or appreciated without knowledge of the longstanding tradition of Indigenous Christianity and its origins. Beginning in 1829, Indigenous leadership within the Methodist Episcopal church in Upper Canada used the Christian Guardian to tell the story of their work among Indigenous communities. These Indigenous accounts of mission work provide a window into how early Indigenous converts to Methodism understood their faith and its meaning within the context of Canadian colonial Christianity, an understanding that differed in significant ways from that of their settler co-religionists. The early Indigenous narrative found in the settler Methodist publication emphasized Indigenous leadership, Indigenous language and the compatibility of Indigenous and Christian spiritual teachings. This study provides an important perspective which confirms and challenges contemporary views on Indigenous Christianity in Canada and helps to reimagine the past, present and future of Christianity in postcolonial contexts.
Dynamic Joint Stiffness of the Knee in Post-Menopausal Women with and without Rheumatoid Arthritis
Authors: Aleixo Pedro , Fernandes Orlando , Patto José Vaz , Abrantes João
Published: January 2023

This study compared rheumatoid arthritis (RA) post-menopausal women with pathological involvement of the lower limb joints and age-matched post-menopausal women without RA regarding the dynamic joint stiffness (DJS) of knee during the stance phase of gait. Eighteen RA women and eighteen age-matched women were selected. Gait assessed through a three-dimensional motion analysis system synchronized with a force plate. Subjects walked barefoot at self-selected speed, and 14 valid trials were collected (comprising 7 left and 7 right foot-steps on force plate). The “moment of force—angle” plot of knee in sagittal plane was determined. The stance phase was split into three sub-phases: first knee flexion sub-phase (1st KFS); knee extension sub-phase (KES); second knee flexion sub-phase (2nd KFS). A linear model represented each sub-phase and DJS calculated by the slope. Model fitting was assessed through the coefficient of determination (R2). R2 values for both groups were higher than 0.8 during 1st KFS and KES but not during 2nd KFS. RA women yielded a higher DJS value during 2nd KFS (p < 0.01). Concerning the other sub-phases, no differences were observed between groups. The findings suggested the splitting methodology used could be modelled by a linear “moment of force—angle” relationship, namely, during 1st KFS and KES. During 2nd KFS, RA women yielded a stiffer behavior.
Forward-Looking Super-Resolution Imaging of MIMO Radar via Sparse and Double Low-Rank Constraints
Authors: Tang Junkui , Liu Zheng , Ran Lei , Xie Rong , Qin Jikai
Published: January 2023

Multiple-input multiple-output (MIMO) radar uses waveform diversity technology to form a virtual aperture to improve the azimuth resolution of forward-looking imaging. However, the super-resolution imaging capability of MIMO radar is limited, and the resolution can only be doubled compared with the real aperture. In the radar forward-looking image, compared with the whole imaging scene, the target only occupies a small part. This sparsity of the target distribution provides the feasibility of applying the compressed sensing (CS) method to MIMO radar to further improve the forward-looking imaging resolution. At the same time, the forward-looking imaging method for a MIMO radar based on CS has the ability to perform single snapshot imaging, which avoids the problem of a motion supplement. However, the strong noise in the radar echo poses a challenge to the imaging method based on CS. Inspired by the low-rank properties of the received radar echoes and the generated images, and considering the existing information about sparse target distribution, a forward-looking super-resolution imaging model of a MIMO radar that combines sparse and double low-rank constraints is established to overcome strong noise and achieve robust forward-looking super-resolution imaging. In order to solve the multiple optimization problem, a forward-looking image reconstruction method based on the augmented Lagrangian multiplier (ALM) is proposed within the framework of the alternating direction multiplier method (ADMM). Finally, the results of the simulation and the measurement data show that the proposed method is quite effective at improving the azimuth resolution and robustness of forward-looking radar imaging compared with other existing methods.