bluuebunny/crossref_metadata_2025 huggingface dataset:
Feasibility, Efficacy, and Safety of Percutaneous Muscle Biopsies in Patients With Chronic Liver Disease
Authors: Quinlan Jonathan I. , Dhaliwal Amritpal , Williams Felicity , Allen Sophie L. , Breen Leigh , Greig Carolyn A. , Lord Janet M. , Armstrong Matthew J. , Elsharkawy Ahmed M.
Published: February 2022

Introduction

Sarcopenia is present in many chronic disease states including decompensated end stage liver disease (ESLD) and non-cirrhotic non-alcoholic fatty liver disease (NAFLD). Sarcopenia in ESLD can negatively impact quality of life and increase mortality. Despite this, very little is understood about the mechanisms of sarcopenia in these conditions. One key reason for this is the reluctance to undertake percutaneous muscle biopsies due to the perceived increased risks. ESLD can induce thrombocytopaenia and coagulopathy which significantly increases the risk of bleeding. In addition, patients with either NAFLD or ESLD often have co-morbidities that would require additional care and risk assessment. Thus, the aim of this study was to establish an effective and safe protocol for the implementation of percutaneous muscle biopsies in patients with NAFLD and ESLD.

Methods

A total of 47 patients with ESLD and 9 patients with non-cirrhotic NAFLD were recruited from the Liver Unit, Queen Elizabeth Hospital (Birmingham, United Kingdom). A total of 71 percutaneous vastus lateralis biopsies were attempted over two study visits. A vigorous safety screening occurred prior to and during each visit and a strict protocol was followed to mitigate against complications and risk.

Results

A total of 85% of patients consented to the muscle biopsy at either visit (48/56). A total of 9% of consented biopsies could not occur due to medical considerations, including high international normalised ratio (INR) (n = 3) and the use of aspirin (n = 4). Muscle tissue was obtained from 90% of attempts, with a mean average yield (wet weight tissue) of 98.1 ± 52.9 mg.

Conclusion

Percutaneous muscle biopsies are both feasible and yield sufficient tissue in an ESLD population. The procedure is effective for obtaining muscle tissue whilst also safe, with only one adverse event. This study provides evidence for the successful use of muscle biopsies in this population, even in consideration of disease specific complications, medications, and comorbidities.
Convergent Loss of Prothoracicotropic Hormone, A Canonical Regulator of Development, in Social Bee Evolution
Authors: Costa Claudinéia P. , Okamoto Naoki , Orr Michael , Yamanaka Naoki , Woodard S. Hollis
Published: February 2022

The evolution of insect sociality has repeatedly involved changes in developmental events and their timing. Here, we propose the hypothesis that loss of a canonical regulator of moulting and metamorphosis, prothoracicotropic hormone (PTTH), and its receptor, Torso, is associated with the evolution of sociality in bees. Specifically, we posit that the increasing importance of social influences on early developmental timing in social bees has led to their decreased reliance on PTTH, which connects developmental timing with abiotic cues in solitary insects. At present, the evidence to support this hypothesis includes the absence of genes encoding PTTH and Torso from all fully-sequenced social bee genomes and its presence in all available genomes of solitary bees. Based on the bee phylogeny, the most parsimonious reconstruction of evolutionary events is that this hormone and its receptor have been lost multiple times, across independently social bee lineages. These gene losses shed light on possible molecular and cellular mechanisms that are associated with the evolution of social behavior in bees. We outline the available evidence for our hypothesis, and then contextualize it in light of what is known about developmental cues in social and solitary bees, and the multiple precedences of major developmental changes in social insects.
Graphene Oxide, a Novel Nanomaterial as Soil Water Retention Agent, Dramatically Enhances Drought Stress Tolerance in Soybean Plants
Authors: Zhao Lin , Wang Wei , Fu Xiaohong , Liu An , Cao Jinfeng , Liu Jianfeng
Published: February 2022

Drought is one of the most severe environmental stressors that place major constraints on the growth of soybeans (Glycine max L.). Graphene oxide (GO) is a nanomaterial that can promote plant growth without toxic effects. In this study, the physiological and molecular responses to drought stress with GO treatment were examined. We discovered that the relative water content (RWC) of stems and leaves treated with GO was 127 and 128% higher than that of the WT plants, respectively. The root parameters in GO-treated soybeans were increased by 33, 38, 34, and 35% than WT plants in total root length, root surface area, root diameter, and root volume, respectively. The activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) were also increased by 29, 57, 28, and 66%, respectively. However, the relative conductivity (REC), malondialdehyde (MDA), and hydrogen peroxide (H2O2) accumulation were remarkably decreased. Furthermore, the content of drought-related hormones JA, SA, and ABA in GO-treated soybeans increased by 32, 34, and 67% than WT plants, respectively. At the molecular level, the effects of GO treatment were manifested by relatively higher expression of four drought-related genes: GmP5CS, GmGOLS, GmDREB1, and GmNCED1. Taken together, our findings revealed that GO could directly increase plant defense enzymes, hormone content, and the expression of drought-related genes, thereby improving the soybean’s ability to resist drought. These findings could provide new opportunities for improving drought tolerance in soybeans through effective soil water retention agents.
Transcriptomic and Physiological Analysis Reveals the Responses to Auxin and Abscisic Acid Accumulation During Vaccinium corymbosum Flower Bud and Fruit Development
Authors: Liu Liangmiao , Zheng Yiqi , Feng Shiji , Yu Lei , Li Yongqiang , Zong Yu , Chen Wenrong , Liao Fanglei , Yang Li , Guo Weidong
Published: February 2022

Blueberry (Vaccinium corymbosum) is reputed as a rich source of health-promoting phytonutrients, which contributes to its burgeoning consumer demand and production. However, blueberries are much smaller and have lower yields than most domesticated berries, and the inherent regulatory mechanisms remain elusive. In this study, the cytological and physiological changes, as well as comparative transcriptomic analysis throughout flower and fruit development in the southern highbush blueberry cultivar ‘O’Neal’ were performed. ‘O’Neal’ hypanthium and fruit exhibited a distinctive cell proliferation pattern, and auxin accumulation was unusual throughout development, while abscisic acid (ABA) levels rapidly increased in association with anthocyanin accumulation, total phenolic reduction and fruit maturation. Transcriptomic data showed that many differentially expressed genes (DEGs) were specifically expressed at each flower bud and fruit developmental stage. Further weighted gene co-expression network analysis (WGCNA) revealed numerous DEGs that correlated with the cell numbers of outer mesocarp and columella, showed two distinctive expression patterns. Most of the DEGs involved in auxin biosynthesis, transportation and signal transduction were upregulated, and this upregulation was accompanied by cell expansion, and flower bud and fruit development. However, individual members ofVcSAUR50andVcIAA9families might be insensitive to auxin, suggesting that these genes play a distinctive role in the growth and development of blueberry fruits. These results will support future research to better understand the flower and fruit development of southern highbush blueberry.
CG and CHG Methylation Contribute to the Transcriptional Control of OsPRR37-Output Genes in Rice
Authors: Liu Chuan , Li Na , Lu Zeping , Sun Qianxi , Pang Xinhan , Xiang Xudong , Deng Changhao , Xiong Zhengshuojian , Shu Kunxian , Yang Fang , Hu Zhongli
Published: February 2022

Plant circadian clock coordinates endogenous transcriptional rhythms with diurnal changes of environmental cues. OsPRR37, a negative component in the rice circadian clock, reportedly regulates transcriptome rhythms, and agronomically important traits. However, the underlying regulatory mechanisms of OsPRR37-output genes remain largely unknown. In this study, whole genome bisulfite sequencing and high-throughput RNA sequencing were applied to verify the role of DNA methylation in the transcriptional control of OsPRR37-output genes. We found that the overexpression of OsPRR37 suppressed rice growth and altered cytosine methylations in CG and CHG sequence contexts in but not the CHH context (H represents A, T, or C). In total, 35 overlapping genes were identified, and 25 of them showed negative correlation between the methylation level and gene expression. The promoter of the hexokinase gene OsHXK1 was hypomethylated at both CG and CHG sites, and the expression of OsHXK1 was significantly increased. Meanwhile, the leaf starch content was consistently lower in OsPRR37 overexpression lines than in the recipient parent Guangluai 4. Further analysis with published data of time-course transcriptomes revealed that most overlapping genes showed peak expression phases from dusk to dawn. The genes involved in DNA methylation, methylation maintenance, and DNA demethylation were found to be actively expressed around dusk. A DNA glycosylase, namely ROS1A/DNG702, was probably the upstream candidate that demethylated the promoter of OsHXK1. Taken together, our results revealed that CG and CHG methylation contribute to the transcriptional regulation of OsPRR37-output genes, and hypomethylation of OsHXK1 leads to decreased starch content and reduced plant growth in rice.
Changes in Candidate Selection and the Sociodemographic Profile of Greek MPs. Evidence From the 2019 General Elections
Authors: Kakepaki Manina
Published: February 2022

The July 2019 national elections in Greece marked the return in power of the conservative party of ND, one of the two pillars of the traditional Greek bipartisanism. Turnover in these elections nearly reached 40%; more than two thirds of the current Parliament MPs were first elected during the crisis, since old parliamentarians slowly give away their seats to newcomers. The aim of this paper is twofold: 1) explore candidate selection mechanisms of old and new parties in Greece inquiring what -if any- has changed in these mechanisms after the Great Recession and whether they adopt IPD in a wider extent; and 2) investigate the sociodemographic profile of newcomers vis-à-vis older Parliamentarians in order to check if the outcome of the elections has changed in terms of a more socially diverse profile. Given that the issue of candidate selection (and election) is mostly based on unwritten rules, our findings will rely on written party rules (such as party manifestos), on original sociodemographic data and on personal interviews. We tentatively suggest that not much has changed in the candidate selection mechanisms in Greece. ND made limited use of its open registry of candidates, whilst SYRIZA applied the same rules as in previous elections. We conclude that, the crisis in Greece offered the opportunity structures for the mass renewal of its parliamentary elite and for a somewhat more socially diverse pool of successful candidates, but its effect quickly disappeared since new MPs resemble more independent political entrepreneurs and have less social and political ties.
Developmental Trajectories in Diagnostic Reasoning: Understanding Data Are Confounded Develops Independently of Choosing Informative Interventions to Resolve Confounded Data
Authors: Moeller April , Sodian Beate , Sobel David M.
Published: February 2022

Two facets of diagnostic reasoning related to scientific thinking are recognizing the difference between confounded and unconfounded evidence and selecting appropriate interventions that could provide learners the evidence necessary to make an appropriate causal conclusion (i.e., the control-of-variables strategy). The present study investigates both these abilities in 3- to 6-year-old children (N= 57). We found both competence and developmental progress in the capacity to recognize that evidence is confounded. Similarly, children performed above chance in some tasks testing for the selection of a controlled test of a hypothesis. However, these capacities were unrelated, suggesting that preschoolers’ nascent understanding of the control-of-variables strategy may not be driven by a metacognitive understanding that confounded evidence does not support a unique causal conclusion, and requires further investigation.
Outpatient Cardiac Rehabilitation Closure and Home-Based Exercise Training During the First COVID-19 Lockdown in Austria: A Mixed-Methods Study
Authors: Kulnik Stefan Tino , Sareban Mahdi , Höppchen Isabel , Droese Silke , Egger Andreas , Gutenberg Johanna , Mayr Barbara , Reich Bernhard , Wurhofer Daniela , Niebauer Josef
Published: February 2022

Objective

To assess the impact of the closure of group-based cardiac rehabilitation (CR) training during the first COVID-19 lockdown in spring 2020 on patients’ physical activity, cardiorespiratory fitness, and cardiovascular risk, and to describe the patient experience of lockdown and home-based exercise training during lockdown.

Design

Mixed methods study. Prospectively collected post-lockdown measurements were compared to pre-lockdown medical record data. Quantitative measurements were supplemented with qualitative interviews about the patient experience during lockdown.

Setting

Outpatient CR centre in Salzburg, Austria.

Participants

Twenty-seven patients [six female, mean (SD) age 69 (7.4) years] who attended weekly CR training sessions until the first COVID-19 lockdown in March 2020.

Outcome Measure(s)

Quantitative: exercise capacity (maximal ergometer test, submaximal ergometer training), cardiovascular risk (Framingham risk score, blood pressure, body mass index, lipids). Qualitative: individual semi-structured interviews.

Results

Exercise capacity had significantly reduced from pre- to post-lockdown: mean (SD) power (W) in maximal ergometry 165 (70) vs. 151 (70), p < 0.001; submaximal ergometer training 99 (40) vs. 97 (40), p = 0.038. There was no significant difference in Framingham risk score and other cardiovascular risk factors. Qualitative data showed that almost all patients had kept physically active during lockdown, but 17 (63%) said they had been unable to maintain their exercise levels, and 15 (56%) felt their cardiorespiratory fitness had deteriorated. Many patients missed the weekly CR training and the motivation and sense of community from training together with others. Several patients stated that without professional supervision they had felt less confident to carry out home-based exercise training at high intensity.

Conclusion

This study highlights the importance of group-based supervised exercise training for patients who engage well in such a setting, and the detrimental impact of disruption to this type of CR service on physical activity levels and exercise capacity. Additionally, learning from the COVID-19 pandemic may inform the development and implementation of remote CR modalities going forward.
How Robust Is Discourse Processing for Native Readers? The Role of Connectives and the Coherence Relations They Convey
Authors: Wetzel Mathis , Zufferey Sandrine , Gygax Pascal
Published: February 2022

While corpus studies have shown that discourse connectives that convey the same coherence relation can display subtle differences, research on online discourse processing has only focused on a rather limited set of connectives. Yet, different connectives – for example, rare or polyfunctional ones – might elicit different reading patterns. In order to explore this assumption, we test the robustness of discourse processing for French native speakers by measuring the way they process causal and concessive sentences that are conveyed by either an appropriate or inappropriate connective. Throughout three experiments, we change important characteristics of the connectives: we first test frequently used connectives (Experiment 1), secondly less frequent ones (Experiment 2), and finally less frequent connectives that are polyfunctional and for which different functions clearly compete (Experiment 3). Our results show that the processing for incoherent items was affected for all connectives, however readers showed altered reading fluency when infrequent connectives were used. We conclude that discourse processing is quite robust and that readers are able to insert meaning conveyed by rare connectives while still showing the highest reading ease with frequent connectives.
Properties of Central and Peripheral Concepts of Emotion in Japanese and Korean: An Examination Using a Multi-Dimensional Model
Authors: Park Eun-Joo , Kikutani Mariko , Suzuki Naoto , Ikemoto Machiko , Lee Jang-Han
Published: February 2022

The concept of emotion can be organized within a hypothetical space comprising a limited number of dimensions representing essential properties of emotion. The present study examined cultural influences on such conceptual structure by comparing the performance of emotion word classification between Japanese and Korean individuals. Two types of emotional words were used; central concepts, highly typical examples of emotion, and less typical peripheral concepts. Participants classified 30 words into groups based on conceptual similarity. MDS analyses revealed a three-dimensional structure with valence, social engagement, and arousal dimensions for both cultures, with the valence dimension being the most salient one. The Japanese prioritized the social engagement over the arousal while the Koreans showed sensitivities to the arousal dimension. Although the conceptual structure was similar for the two countries, the weight of importance among the three dimensions seems to be different, reflecting each culture’s values and communication styles.
Effect of Exposure to Socio-Political Violence and Abuse During Childhood on Executive Planning in Adult Life
Authors: Montoya-Arenas David Andrés , Londoño-Guzmán Daniel , Franco José G. , Gaviria Ana M.
Published: February 2022

Objective

The exposure to unfavorable environments during childhood negatively affects the development of the executive planning abilities in adult life. In countries with sociopolitical conflicts, children are exposed to traumatic events as a result of child abuse and sociopolitical violence. The purpose of this study was to analyze the effect of the exposure to both forms of adverse childhood experiences on the executive planning abilities in adults from the general population.

Method

The history of child abuse and sociopolitical violence during childhood was assessed, as well as the executive planning abilities, in 59 adults older than 49 without cognitive impairment or depressive disorder.

Results

Of the sample, 88.1% experienced at least one child abuse event and 47.5% was exposed to sociopolitical violence. Sexual abuse and physical abuse (child abuse) were associated with reduced performance in executive planning. Forced displacement and extortion (sociopolitical violence) had a mixed relationship with planning ability, improving some aspects, and worsening some others. Kidnapping was associated with increased capacity and control of the working memory and executive planning.

Conclusions

The traumatic events during childhood have differential effects on the executive planning skills in the adult life. The exposure to sexual and physical abuse negatively affects executive skills; on the other hand, sociopolitical violence has a mixed or positive impact. Specifically, kidnapping favors the executive planning processes, probably under an evolutionary adaptive mechanism.
Occurrence of Psychiatric Disorders, Self-Sufficiency Problems and Adverse Childhood Experiences in a Population Suspected of Violent Extremism
Authors: Grimbergen Christel , Fassaert Thijs
Published: February 2022

Background

Public health-inspired programs for Countering Violent Extremism (CVE) have developed internationally in a relatively short period of time. Research into these programs is scarce. There is a need for information that helps drive public health interventions.

Objectives

To present data on the occurrence of psychiatric disorders, self-sufficiency problems and adverse childhood experiences (ACE) in a population suspected of violent extremism.

Methods

A cross-sectional study, with data from screening reports for 34 adult subjects included in a multi-agency case-based approach on violent extremism in Amsterdam, the Netherlands. Subjects were screened in the period between December 2015 to May 2021. Screening reports, which included the Screener for Intelligence and Learning Disabilities (SCIL) and the Dutch version of the Self-sufficiency Matrix (SSM-D), were used to gather information on the main outcome measures.

Results

Major psychiatric disease categories were found to be mood and anxiety disorders and mild intellectual disability (each 29.4%), substance related disorders (35.3%), personality disorders (41.2%), and psychotic disorders (14.7%). Complex self-sufficiency problems, measured by the number of people who had self-sufficiency problems in 4+ domains and the number of people who had similar self-sufficiency problems as homeless people in Amsterdam, were found in 35.3 and 32.4% of the client sample. The most prevalent ACE were emotional neglect (47.1%), household mental illness (44.1%), and loss of a parent (38.2%), 35.3% had been exposed to 4+ ACE. An association was found between NACE and self-sufficiency problems on two domains, namely “Mental Health” (rho = 0.51, p = 0.002) and “Law and order” (rho = 0.42, p = 0.013).

Conclusions

An accumulation of social and psychiatric problems in people suspected of violent extremism underlines the importance of professionals in health and social care being actively involved in developing CVE approaches.
A Novel Extra Tree Ensemble Optimized DL Framework (ETEODL) for Early Detection of Diabetes
Authors: Arya Monika , Sastry G Hanumat , Motwani Anand , Kumar Sunil , Zaguia Atef
Published: February 2022

Diabetes has been recognized as a global medical problem for more than half a century. Patients with diabetes can benefit from the Internet of Things (IoT) devices such as continuous glucose monitoring (CGM), intelligent pens, and similar devices. Smart devices generate continuous data streams that must be processed in real-time to benefit the users. The amount of medical data collected is vast and heterogeneous since it is gathered from various sources. An accurate diagnosis can be achieved through a variety of scientific and medical techniques. It is necessary to process this streaming data faster to obtain relevant and significant knowledge. Recently, the research has concentrated on improving the prediction model's performance by using ensemble-based and Deep Learning (DL) approaches. However, the performance of the DL model can degrade due to overfitting. This paper proposes the Extra-Tree Ensemble feature selection technique to reduce the input feature space with DL (ETEODL), a predictive framework to predict the likelihood of diabetes. In the proposed work, dropout layers follow the hidden layers of the DL model to prevent overfitting. This research utilized a dataset from the UCI Machine learning (ML) repository for an Early-stage prediction of diabetes. The proposed scheme results have been compared with state-of-the-art ML algorithms, and the comparison validates the effectiveness of the predictive framework. This proposed work, which outperforms the other selected classifiers, achieves a 97.38 per cent accuracy rate. F1-Score, precision, and recall percent are 96, 97.7, and 97.7, respectively. The comparison unveils the superiority of the suggested approach. Thus, the proposed method effectively improves the performance against the earlier ML techniques and recent DL approaches and avoids overfitting.
Profiling the New Zealand Police Trainee Physical Competency Test
Authors: Dawes J. Jay , Scott Jordan , Canetti Elisa F. D. , Lockie Robert G. , Schram Ben , Orr Robin M.
Published: February 2022

Police officers require a certain amount of occupational fitness to successfully perform physically demanding tasks. As such, trainees are required to undergo training to develop their ability to perform such tasks. The physical competency test (PCT) is a 400 m obstacle course consisting of key police occupational physical tasks used to evaluate a trainee's ability to complete tasks that a police officer is expected to perform whilst on duty. The purpose of this study was to profile the PCT in a police recruit population to provide an indication of the current level of occupational fitness within a policing population to inform conditioning requirements. Retrospective data for 813 male (age = 27.41 ± 5.92 years, body mass = 83.98 ± 14.03 kg, height = 179.23 ± 10.50 cm, BMI = 25.85 ± 3.92 kg/m2) and 372 female (mean age = 27.01 ± 6.45 years, mean weight = 67.14 ± 8.60 kg, mean height = 168.14 ± 6.46 cm and mean BMI = 23.61 ± 2.52 kg/m2) police trainees from the New Zealand Police Constabulary Recruitment database were provided for analysis. Anthropometric data, including height, body mass, and BMI were provided, in addition to trainee PCT time. Data were split by sex and age. Significant differences were observed between sexes for all anthropometric measures and PCT time (p< 0.001). Generally, in both the male and female groups, younger recruits tended to perform better than the older recruits with results between the “under 20” and the 20–24-year-old-age groups performing significantly better than the 35–39-year-old-age group in both sexes, and the 25–29-year-old-age group performing significantly better than the 35–39-year-old-age group in female officers. The data provided in this study provides a profile for performance of male and female recruits of various ages on the PCT in preparation for entry, or re-entry following injury, into the NZ Police. However, given that the PCT is considered a measure of occupational task performance, consideration should be given to the use of sex and age neutral requirements as the occupational tasks performed by police officers exhibit the same traits regardless of sex or age. Older trainees may therefore need conditioning to improve PCT times and subsequently occupational performance.
Chronic and Residual Effects of a Two-Week Foam Rolling Intervention on Ankle Flexibility and Dynamic Balance
Authors: Seever Thomas Christoph , Mason Joel , Zech Astrid
Published: February 2022

Background

Foam rolling has been shown to acutely improve joint range of motion (ROM). However, limited knowledge exists on the chronic and residual effects. The primary purpose of this study was to examine the chronic and residual effects of a 2-week roller–massager intervention on ankle dorsiflexion ROM and dynamic balance.

Methods

Forty-two participants (24.3 ± 2.5 years, 33 males, 9 females) were randomly assigned to either roller-massage (RM) or control group (= no intervention). Ankle ROM was assessed with the weight-bearing lunge test (WBLT) and dynamic balance with the Y-Balance test for both limbs. The RM group was instructed to roll their calf muscles for three sets of 60 s per leg on 6 days a week over 2 weeks. Acute effects were measured during baseline testing for dorsiflexion ROM and dynamic balance immediately after foam rolling. Chronic and residual effects were measured 1 day and 7 days after the intervention period. Multivariate ANOVA was performed for post-hoc comparisons to determine acute, chronic, and residual effects.

Results

Significant acute and chronic foam rolling effects (p <0.05) were found for ankle dorsiflexion ROM. The chronic increase in ROM slightly decreased 7 days post-intervention but remained significantly above baseline (p < 0.05). Regarding dynamic balance, there were no acute but chronic (p < 0.05) and residual (p < 0.05) effects.

Conclusion

Using a roller–massager for a 2-week period chronically increases ROM and dynamic balance. These increases are still significant 7 days post-intervention emphasizing the sustainability of foam rolling effects.
Metabolic Reprogramming, Gut Dysbiosis, and Nutrition Intervention in Canine Heart Disease
Authors: Li Qinghong
Published: February 2022

This review provides a state-of-the-art overview on recent advances in systems biology in canine cardiac disease, with a focus on our current understanding of bioenergetics and amino acid metabolism in myxomatous mitral valve disease (MMVD). Cross-species comparison is drawn to highlight the similarities between human and canine heart diseases. The adult mammalian heart exhibits a remarkable metabolic flexibility and shifts its energy substrate preference according to different physiological and pathological conditions. The failing heart suffers up to 40% ATP deficit and is compared to an engine running out of fuel. Bioenergetics and metabolic readaptations are among the major research topics in cardiac research today. Myocardial energy metabolism consists of three interconnected components: substrate utilization, oxidative phosphorylation, and ATP transport and utilization. Any disruption or uncoupling of these processes can result in deranged energy metabolism leading to heart failure (HF). The review describes the changes occurring in each of the three components of energy metabolism in MMVD and HF. It also provides an overview on the changes in circulating and myocardial glutathione, taurine, carnitines, branched-chain amino acid catabolism and tryptophan metabolic pathways. In addition, the review summarizes the potential role of the gut microbiome in MMVD and HF. As our knowledge and understanding in these molecular and metabolic processes increase, it becomes possible to use nutrition to address these changes and to slow the progression of the common heart diseases in dogs.
Sequence Analysis of Macaca mulatta TRIM4 and Its Role in the Interferon Pathway
Authors: Zhao Mengmeng , Li Huawei , Zhang Hang , Sha Huiyang , Huang Liangzong , Wang Ruining
Published: February 2022

Monkey diseases are becoming increasingly severe, and some may be transmitted to humans through direct and indirect contact. Innate immunity is the first line of defense against foreign microorganisms. It is of great significance to explore the immune characteristics of monkey and human diseases. TRIM4, an important immune molecule in Macaca mulatta, was cloned and its immunological characteristics were preliminarily explored. The results showed that Macaca mulatta TRIM4 was in the same branch as human TRIM4. Overexpression of TRIM4 increased the mRNA levels of interferon (IFN)-alpha, IFN-beta, RIG-I, MAVS, IRF3, IRF7, OAS1, IFIT3, and CCL5, TRIM4 up-regulated the activities of IFN-beta, NF-κB, and ISRE reporter. In contrast, inhibiting TRIM4 expression by small interfering RNA (siRNA) down-regulated the IFN pathway. In summary, Macaca mulatta TRIM4 plays an essential role in the IFN pathway.
A Two-Party Quantum Parliament
Authors: Andronikos Theodore , Stefanidakis Michail
Published: February 2022

This paper introduces the first functional model of a quantum parliament that is dominated by two parties or coalitions, and may or may not contain independent legislators. We identify a single crucial parameter, aptly named free will radius, which can be used as a practical measure of the quantumness of the parties and the parliament as a whole. The free will radius used by the two parties determines the degree of independence that is afforded to the representatives of the parties. Setting the free will radius to zero degrades the quantum parliament to a classical one. On the other hand, setting the free will radius to its maximum value 1 makes the representatives totally independent. Moreover, we present a quantum circuit in Qiskit with which we simulate the operation of the quantum parliament under various scenarios. The experimental results allow us to arrive at some novel and fundamental conclusions that, we believe, provide new insights into the operation and the traits of a possible future quantum parliament. Finally, we propose the game “Passing the Bill,” which captures the operation of the quantum parliament and basic options available to the leadership of the two parties.
Long-Term Impacts of Different Cropping Patterns on Soil Physico-Chemical Properties and Enzyme Activities in the Low Land Plain of North China
Authors: Gikonyo Florence Nyambura , Dong Xinliang , Mosongo Peter Semba , Guo Kai , Liu Xiaojing
Published: February 2022

Various cropping patterns are extensively used on the North China Plain, which greatly alter various soil properties. Although these soil properties respond differently to the different cropping patterns, little is known about the possible effects of cropping patterns on desalinized soils. To assess the effects of the different cropping patterns on soil properties and enzyme activities, a long-term field experiment was conducted at the Nanpi Agro-Ecological Station in Hebei Province, China. The cropping patterns under study were the single-cropping patterns of winter wheat (Triticum aestivum L.), spring maize (Zea mays), and spring cotton (Gossypium hirsutum), and the double-cropping pattern of winter wheat–summer maize (WW–SM), which is the predominant cropping pattern on the North China Plain. Soil samples were collected at a depth of 0–100 cm, depending on the soil properties. Our results indicated that soil available phosphorous (P) and available potassium (K) concentrations at 0–20 cm were greater than those of the subsurfaces. Soil organic matter declined with depth, and WW–SM had the highest amount in the deep levels. The WW–SM cropping pattern also generally showed greater levels of enzyme activities than did the single-cropping patterns, proving that diverse crops can enhance enzyme activities. Soil pH generally increased with depth despite there being no significant differences between the cropping patterns. No significant effects were observed on soil electrical conductivity (EC). However, EC generally increased with depth in spring cotton and winter wheat and declined in spring maize and WW–SM. Winter wheat had a significant aggregate size (<0.053 mm) at 20–40 cm depth and generally showed the lowest amount of nutrients. This study suggests that a fallow period allows the soil to regenerate its structure, whereas WW–SM enhances high soil organic matter in the deep soil layers and promotes increased soil enzyme activities. This indicates that crop rotation could sustainably exploit soil resources without reducing fertility over a long period. There is a need to incorporate different soil management practices for single-cropping patterns to increase their productivity, especially in spring maize, whose organic matter declined the most. Our results also show a need to diversify to different crop rotations to utilize their benefits and enhance soil productivity while increasing crop output.
The Sorption of Sulfamethoxazole by Aliphatic and Aromatic Carbons from Lignocellulose Pyrolysis
Authors: Chu Gang , Han Zifeng , Wang Zimo , Kong Defeng , Qin Wenxiu , Si Youbin , Wang Guozhong , Steinberg Christian E. W.
Published: February 2022

Massive biomass waste with lignocellulose components can be used to produce biochar for environmental remediation. However, the impact of lignocellulose pyrolysis on biochar structure in relation to the sorption mechanism of ionizable antibiotics is still poorly understood. In this paper, diverse techniques including thermogravimetric analysis and 13C nuclear magnetic resonance were applied to investigate the properties of biochars as affected by the pyrolysis of cellulose and lignin in feedstock. Cellulose-derived biochars possessed more abundant groups than lignin-derived biochars, suggesting the greater preservation of group for cellulose during the carbonization. Higher sorption of sulfamethoxazole (SMX) was also observed by cellulose-derived biochars owing to hydrogen bond interaction. Sorption affinity gradually declined with the conversion aliphatic to aromatic carbon, whereas the enhanced specific surface area (SSA) subsequently promoted SMX sorption as evidenced by increased SSA-N2 and SSA-CO2 from 350 to 450 °C. The decreased Kd/SSA-N2 values with increasing pH values implied a distinct reduction in sorption per unit area, which could be attributed to enhanced electrostatic repulsion. This work elucidated the role of carbon phases from thermal conversion of lignocellulose on the sorption performance for sulfonamide antibiotics, which will be helpful to the structural design of carbonaceous adsorbents for the removal of ionizable antibiotics.
Effect of Eucalyptus-Wood-Based Compost Application Rates on Avocado (Persea americana Mill) Foliar Nutrient Content and Fruit Yield
Authors: Mohale Mmatshaka Phillimon , Manyevere Alen , Parwada Cosmas , Zerizghy Mussie
Published: February 2022

Background: The effects of different fertilizer types and their application rates on leaf nutrient content and avocado yield are unclear. An evaluation of eucalyptus-wood-based compost applied at 0, 5, 10, and 15 t ha−1 year−1 on foliar nutrient content, yield, and fruit size distribution (%) was completed at Mooketsi and Politsi, Limpopo Province, South Africa, from 2016 to 2018. Methods: A completely randomized block design with three replicates was used. Data were collected annually, and fruit size was classified as classes; 1 (>275 g), 2 (197–274 g), 3 (148–196 g), and 4 (0–147 g). Results: Leaf N, P, K, Ca, Mg, Fe, Mn, Na, Cu, and Zn, fruit yield and size distribution (%) were significantly (p < 0.05) affected by compost quantity and proportionally increased with application rates in each year. Compost had cumulative effects on the measured parameters; the highest values were recorded in 2018 under a 15 t ha−1 application rate, which corresponded to the highest yield (19.6 t ha−1) and the largest proportion of fruits in class 1. Conclusions: The ≥10 t ha−1 year−1 application rate can be used, though the compost saturation levels of the soils should be determined to avoid possible danger of nutrient toxicity if high application rates are continuously used for >3 years.
Nitrogen Excretion by Dairy Cows Grazing Plantain (Plantago lanceolata) Based Pastures during the Lactating Season
Authors: Navarrete Soledad , Rodriguez María , Horne David , Hanly James , Hedley Mike , Kemp Peter
Published: February 2022

The use of plantain pasture in dairy systems can potentially reduce nitrogen (N) leaching losses via the lower N concentration in the urine (UNc) of cows. Reducing the urinary N load while cows graze pastures can reduce the risk of N leaching losses from urine patches. Research needs to demonstrate that these environmental benefits are not at the expense of milk production and farm profit. Three groups of 20 cows grazed in the following three pasture treatments: (i) plantain, (ii) plantain–clover mix (plantain, red [Trifolium pratense] and white clover), or (iii) ryegrass-white clover (wc) pastures, from spring to autumn for two years. Each year, pasture intake, diet quality, milk production and animal N (milk and urine) excretion were evaluated in spring, summer, and autumn. The cows grazing the plantain and plantain–clover mix pastures produced similar milk solids as cows grazing ryegrass–wc pasture but reduced their UNc during summer and autumn, when compared to those grazing the plantain–clover mix and ryegrass–wc pastures. Plantain reduced urinary N loads onto pastures by a greater number of urine patches with lower urinary N loading rates. The results demonstrate that plantain pastures do not diminish milk solids production from cows, and the lower UNc from summer to autumn could reduce N being lost to the environment.
Surgical Antimicrobial Prophylaxis in Neonates and Children with Special High-Risk Conditions: A RAND/UCLA Appropriateness Method Consensus Study
Authors: Bianchini Sonia , Rigotti Erika , Nicoletti Laura , Monaco Sara , Auriti Cinzia , Castagnola Elio , Castelli Gattinara Guido , De Luca Maia , Galli Luisa , Garazzino Silvia , La Grutta Stefania , Lancella Laura , Lo Vecchio Andrea , Maglietta Giuseppe , Montagnani Carlotta , Petrosillo Nicola , Pietrasanta Carlo , Principi Nicola , Simonini Alessandra , Tesoro Simonetta , Venturini Elisabetta , Piacentini Giorgio , Lima Mario , Staiano Annamaria , Esposito Susanna ,   on behalf of the Peri-Operative Prophylaxis in Neonatal and Paediatric Age (POP-NeoPed) Study Group
Published: February 2022

Surgical site infections (SSIs), which are a potential complications in surgical procedures, are associated with prolonged hospital stays and increased postoperative mortality rates, and they also have a significant economic impact on health systems. Data in literature regarding risk factors for SSIs in pediatric age are scarce, with consequent difficulties in the management of SSI prophylaxis and with antibiotic prescribing attitudes in the various surgical procedures that often tend to follow individual opinions. The lack of pediatric studies is even more evident when we consider surgeries performed in subjects with underlying conditions that may pose an increased risk of complications. In order to respond to this shortcoming, we developed a consensus document to define optimal surgical antimicrobial prophylaxis (SAP) in neonates and children with specific high-risk conditions. These included the following: (1) colonization by methicillin-resistant Staphylococcus aureus (MRSA) and by multidrug resistant (MDR) bacteria other than MRSA; (2) allergy to first-line antibiotics; (3) immunosuppression; (4) splenectomy; (5) comorbidity; (6) ongoing antibiotic therapy or prophylaxis; (7) coexisting infection at another site; (8) previous surgery in the last month; and (9) presurgery hospitalization lasting more than 2 weeks. This work, made possible by the multidisciplinary contribution of experts belonging to the most important Italian scientific societies, represents, in our opinion, the most up-to-date and comprehensive collection of recommendations relating to behaviors to be undertaken in a perioperative site in the presence of specific categories of patients at high-risk of complications during surgery. The application of uniform and shared protocols in these high-risk categories will improve surgical practice with a reduction in SSIs and consequent rationalization of resources and costs, as well as being able to limit the phenomenon of antimicrobial resistance.
3-Hydroxybutyrate Ameliorates the Progression of Diabetic Nephropathy
Authors: Jung Jeeyoun , Park Woo Yeong , Kim Yun Jin , Kim Mikyung , Choe Misun , Jin Kyubok , Seo Ji Hae , Ha Eunyoung
Published: February 2022

Studies report beneficial effects of 3-hydroxybutyrate (3-OHB) on the treatment of type 2 diabetes and obesity, but the effects of 3-OHB on diabetic nephropathy have not been elucidated. This study was designed to investigate the efficacy and mechanism of 3-OHB against progression of diabetic nephropathy (DN). Mice (db/db) were fed normal chow, high-fat, or ketogenic diets (KD) containing precursors of 3-OHB. Hyperglycemia was determined based on random glucose level (≥250 mg/dL). Fasting blood glucose and body weights were measured once a week. Twenty four-hour urine albumin to creatinine ratio was determined 5 weeks after the differential diet. Energy expenditure was measured 9 weeks after the differential diet. Body weights were significantly lower in the KD group than those in other groups, but no significant differences in fasting blood glucose levels among three groups were observed. Urine albumin to creatinine ratio and serum blood urea nitrogen (BUN) to creatinine ratio in the KD group were significantly lower than in other groups. Histologic and quantitative analysis of mesangial area suggested that KD delayed the progression of DN phenotype in db/db mice. Metabolic cage analysis also revealed that KD increased energy expenditure in db/db mice. In vitro studies with proximal tubular cells revealed that 3-OHB stimulated autophagic flux. 3-OHB increased LC3 I to LC3 II ratio, phosphorylation of AMPK, beclin, p62 degradation, and NRF2 expression. Moreover, we found that 3-OHB attenuated high glucose-induced reactive oxygen species (ROS) levels in proximal tubular cells. In vivo study also confirmed increased LC3 and decreased ROS levels in the kidney of KD mice. In summary, this study shows in both in vivo and in vitro models that 3-OHB delays the progression of DN by augmenting autophagy and inhibiting oxidative stress.
Inverse Solution Error Analysis and Correction of Beam Steering System Based on Risley Prisms
Authors: Song Yinuo , Gao Shijie , Wu Jiabin , Wang Shuaifei , Huo Li
Published: February 2022

The pointing accuracy of the Risley prisms beam-steering system mainly depends on the accuracy of the solution method and the impact of the error on the solution. To improve pointing accuracy, the impact of systematic errors on the inverse solution precision is investigated and a correction method is proposed. First, a more accurate error model for Risley prisms is established, and the errors are obtained by the parameter identification method, which corrects the forward solution error. Second, we explain the reason for the error generated by the inverse solution and analyze the variation of the inverse solution error magnitude with the beam deflection angle. A correction method based on pointing-field transformation is proposed. Finally, simulations and experiments are performed to verify the feasibility of the method. Experimental results show that when the beam deflection angle is equal to 0.1°, the maximum and RMS values of the pointing error are reduced by 94.08% and 95.18%, respectively.
Prediction of Liquefaction-Induced Lateral Displacements Using Gaussian Process Regression
Authors: Ahmad Mahmood , Amjad Maaz , Al-Mansob Ramez , Kamiński Paweł , Olczak Piotr , Khan Beenish , Alguno Arnold
Published: February 2022

During severe earthquakes, liquefaction-induced lateral displacement causes significant damage to designed structures. As a result, geotechnical specialists must accurately estimate lateral displacement in liquefaction-prone areas in order to ensure long-term development. This research proposes a Gaussian Process Regression (GPR) model based on 247 post liquefaction in-situ free face ground conditions case studies for analyzing liquefaction-induced lateral displacement. The performance of the GPR model is assessed using statistical parameters, including the coefficient of determination, coefficient of correlation, Nash–Sutcliffe efficiency coefficient, root mean square error (RMSE), and ratio of the RMSE to the standard deviation of measured data. The developed GPR model predictive ability is compared to that of three other known models—evolutionary polynomial regression, artificial neural network, and multi-layer regression available in the literature. The results show that the GPR model can accurately learn complicated nonlinear relationships between lateral displacement and its influencing factors. A sensitivity analysis is also presented in this study to assess the effects of input parameters on lateral displacement.
Improving Serial Detection Using MAP Algorithm for Bit-Patterned Media Recording
Authors: Nguyen Thien An , Lee Jaejin
Published: February 2022

Bit-patterned media recording (BPMR) is a promising technology for increasing the areal density (AD) of magnetic data storage systems. In BPMR, the non-magnetic zone is reduced to increase AD, which causes serious problems such as two-dimensional (2D) interference and track mis-registration (TMR). Therefore, in previous studies, a serial detection, which exploits the Viterbi algorithm (VA), was proposed to combat 2D interference. Because the VA lacks prior information from the inner detector to the outer detector, in this study, we use the maximum a posteriori (MAP) algorithm to supply prior information from the inner detector to the outer detector. Simulation results show that the proposed model can improve the bit error ratio (BER) performance of serial detection, especially in cases where the BPMR suffers from TMR effects.