bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential early response of monocyte/macrophage subsets to intra-operative corticosteroid administration in lung transplantation
Authors: Glorion Matthieu , Pascale Florentina , Huriet Maxime , Estephan Jérôme , Gouin Carla , Urien Céline , Bourge Mickael , Egidy Giorgia , Richard Christophe , Gelin Valérie , De Wolf Julien , Le Guen Morgan , Magnan Antoine , Roux Antoine , Devillier Philippe , Schwartz-Cornil Isabelle , Sage Edouard
Published: October 2023

Introduction

Lung transplantation often results in primary and/or chronic dysfunctions that are related to early perioperative innate allo-responses where myeloid subsets play a major role. Corticosteroids are administered upon surgery as a standard-of-care but their action on the different myeloid cell subsets in that context is not known.

Methods

To address this issue, we used a cross-circulatory platform perfusing an extracorporeal lung coupled to cell mapping in the pig model, that enabled us to study the recruited cells in the allogeneic lung over 10 hours.

Results

Myeloid cells, i.e. granulocytes and monocytic cells including classical CD14pos and non-classical/intermediate CD16pos cells, were the dominantly recruited subsets, with the latter upregulating the membrane expression of MHC class II and CD80/86 molecules. Whereas corticosteroids did not reduce the different cell subset recruitment, they potently dampened the MHC class II and CD80/86 expression on monocytic cells and not on alveolar macrophages. Besides, corticosteroids induced a temporary and partial anti-inflammatory gene profile depending on cytokines and monocyte/macrophage subsets.

Discussion

This work documents the baseline effects of the standard-of-care corticosteroid treatment for early innate allo-responses. These insights will enable further optimization and improvement of lung transplantation outcomes.
Environmental capacity and fluxes of land-sourced pollutants around the Leizhou Peninsula in the summer
Authors: Chen Ying , Sun Yan , Shi Haiyi , Zhao Hui , Gao Hui , Pan Gang , Tian Kai
Published: October 2023

Although the water environment has certain self-purification capability, the natural balance is disrupted, leading to water quality deterioration when the discharge load of wastewater exceeds a certain threshold. This problem implies the urgency of evaluating marine environmental capacity as a necessary parameter for marine sustainable development of marine ecosystems. Through principal component analysis (PCA), clustering, and other methods, we analyzed the average concentration and fluxes of land-sourced pollutants and determined the pollution level around the Leizhou Peninsula. Combined with the Delft3D hydrodynamic numerical model, tidal hydrodynamic forces and pollutants migration and diffusion were calculated. Based on in-situ measured data, the model was validated. The sharing rate method was used to calculate the marine environmental capacity in Zhanjiang Bay and analyzed their impact on seawater eutrophication. The results showed that: (1) The average concentrations of chemical oxygen demand (COD), ammonia nitrogen (NH4+), total nitrogen (TN), and total phosphorus (TP) around Leizhou Peninsula were 22.56 mg/L, 0.69 mg/L, 6.69 mg/L, and 0.69 mg/L, respectively. (2) Six areas (Area A-F) can be divided into, based on the discharge of land-sourced pollutants into the sea area. According to the results of PCA, clustering, and other methods, the average concentration and fluxes of land-sourced pollutants in Area B (i.e. Zhanjiang Bay) were very high. (3) The environmental capacity of Zhanjiang Bay was calculated through Delft3D numerical simulation, and it was found that the COD and TN environmental capacity of 6 sewage outlets exceeded the standard, while the TP environmental capacity of 3 sewage outlets exceeded the standard. (4) According to the statistical research result, most of the Zhanjiang Bay waters has been restricted by nitrogen for over a decade. Therefore, we speculate that although TN environmental capacity exceeds the standard, its impact on eutrophication in Zhanjiang Bay is still limited to a certain extent.
Sensory nerve action potential in patients with functional neurological disorders with sensory manifestations
Authors: Morimoto Kohei , Sekiguchi Kenji , Matsumoto Riki
Published: October 2023

Introduction

Functional neurological disorder (FND) has various clinical manifestations. Even though diagnostic criteria for FND have been proposed, FND characteristics with sensory manifestations have not been elucidated. Therefore, we aimed to investigate the association between sensory nerve action potential (SNAP) amplitudes and FND with sensory manifestations.

Methods

We included 76 outpatients with FND with sensory manifestations whose nerve conduction studies were performed retrospectively. Additionally, we defined 121 patients with other neurological diseases who did not have peripheral neuropathy as disease controls. The SNAP amplitudes were compared between the two groups. We also explored the relationship between SNAP amplitudes and FND-specific clinical symptoms in patients with FND.

Results

No differences were observed in SNAP amplitudes adjusted for age between patients with FND who had sensory manifestations and disease control patients. Additionally, no differences were observed between patients with FND who had and did not have FND-specific clinical symptoms.

Conclusion

The SNAP amplitude in patients with FND who had sensory manifestations was equivalent to that in controls.
Interaction effects between smoking and internet gaming disorder on resting-state functional connectivity of the ventral tegmental area and hippocampus
Authors: Qiu Xianxin , Han Xu , Wang Yao , Ding Weina , Sun Yawen , Lei Hao , Zhou Yan , Lin Fuchun
Published: October 2023

Background

Many reports have focused on cigarette smoking and internet gaming disorder (IGD), with widespread alterations of resting-state functional connectivity (rsFC) in the reward and memory circuits, respectively. Epidemiological studies have also shown high comorbidity of cigarette smoking and IGD. However, the underlying mechanisms are still unknown. Therefore, this study investigates the comorbidity and interaction effects between smoking and IGD from the rsFC perspective.

Methods

Resting-state functional magnetic imaging data were collected from 60 healthy controls (HC), 46 smokers, 38 IGD individuals, and 34 IGD comorbid with smoking (IGDsm) participants. Voxel-wise rsFC maps were calculated for all subjects with the ventral tegmental area, rostral hippocampus, and caudal hippocampus as regions of interest, respectively.

Results

Significant interaction effects between smoking and IGD were mainly involved in the reward and memory circuits; that is, the rsFC between the ventral tegmental area and right nucleus accumbens, between the rostral hippocampus and bilateral nucleus accumbens, sensorimotor areas, and left middle temporal gyrus. Specifically, in these circuits, smokers showed decreased rsFC compared to the HC group, while IGDsm showed increased rsFC compared to smokers and IGD individuals. The IGDsm and HC groups showed no significant difference. The altered rsFC also correlated with clinical measures.

Conclusion

These findings indicate that lower rsFC in smokers or IGD individuals increases under the effect of another type of addiction, such as smoking and IGD, but only increases to the normal state, which might explain the comorbidity and interaction between smoking and IGD from the perspective of functional circuits.
Case Report: Bilateral targeted intraoperative radiotherapy: a safe and effective alternative for synchronous bilateral breast cancer
Authors: Bhimani Fardeen , McEvoy Maureen , Gupta Anjuli , Pastoriza Jessica , Shihabi Areej , Basavatia Amar , Tomé Wolfgang A. , Fox Jana , Mehta Keyur , Feldman Sheldon
Published: October 2023

Background

The incidence of bilateral breast cancer (BBC) ranges from 1.4% to 11.8%. BBC irradiation is a challenge in current clinical practice due to the large target volume that must be irradiated while minimizing the dose to critical organs. Supine or prone breast techniques can be used, with the latter providing better organ sparing; both, however, result in lengthy treatment times. The use of Intra-operative radiotherapy (IORT) in breast cancer patients who choose breast conservation has been highlighted in previous studies, but there is a scarcity of literature analyzing the utility and applicability of IORT in BBC. This case series aims to highlight the applicability of administering bilateral IORT in patients with BBC.

Case reports

Five patients with bilateral early-stage breast cancer (or DCIS) were treated with breast-conserving surgery followed by bilateral IORT. Of the 10 breast cancers, 8 were diagnosed as either DCIS or IDC, while the other 2 were diagnosed as invasive lobular carcinoma and invasive carcinoma, respectively. During surgery, all patients received bilateral IORT. Furthermore, 1 patient received external beam radiation therapy after her final pathology revealed grade 3 DCIS. The IORT procedure was well tolerated by all five patients, and all patients received aromatase inhibitors as adjuvant therapy. Additionally, none of these patients showed evidence of disease after a 36-month median follow-up.

Conclusion

Our findings demonstrate the successful use of IORT for BCS in patients with BBC. Furthermore, none of the patients in our study experienced any complications, suggesting the feasibility of the use of IORT in BBC. Considering the benefits of improved patient compliance and a reduced number of multiple visits, IORT may serve as an excellent patient-centered alternative for BBC. Future studies are recommended to reinforce the applicability of IORT in patients with BBC.
Assessing changes in range of motion in adolescent patients undergoing myoActivation® for chronic pain related to myofascial dysfunction: a feasibility study
Authors: Bhatnagar Tim , Azim Farah T. , Behrouzian Mona , Davies Karen , Wickenheiser Diane , Jahren Gail , West Nicholas , Leveille Lise , Lauder Gillian R.
Published: October 2023

Introduction

myoActivation® assessment utilizes systemized movement tests to assess for pain and limitations in motion secondary to myofascial dysfunction. myoActivation needling therapy resolves the myofascial components of pain and is associated with immediately observed changes in pain, flexibility, and range of motion. The principal aim of this feasibility study was to objectively characterize the kinematic metrics of upper and lower body motion before and after myoActivation movement tests and therapy.

Methods

Five consecutive eligible adolescent participants considered appropriate for myoActivation were consented to receive their myoActivation intervention in a motion laboratory. Clinical motion analysis was used to measure the changes in maximum range of motion (maxROM) and maximum angular speed to maximum ROM (speedROM) of movement tests predicted to change. Metrics were analyzed to assess changes over specified time intervals - i) baseline to after initial myoActivation session, and ii) baseline to after complete myoActivation course. Each participant served as their own control.

Results

We demonstrated objective evidence of improved maxROM and/or speedROM in 63% of the movement tests predicted to change after just one session of myoActivation and in 77% of movement tests predicted to change over the complete course of treatment. The myoActivation clinician observed positive change in 11/19 of movement tests across all patients, that were predicted to change after the initial myoActivation session; 81% of these positive changes were confirmed by the kinematic data.

Discussion

Clinical motion analysis provides objective support to clinicians evaluating, treating, and teaching myofascial release. A larger, prospective clinical trial is warranted to explore the impact of myoActivation on movement. Refinement of observation techniques and outcome measures established in this feasibility study will strengthen future clinical motion analysis of the myoActivation process.
Vaccination dropout and wealth related inequality among children aged 12–35 months in remote and underserved settings of Ethiopia: a cross-sectional evaluation survey
Authors: Shiferie Fisseha , Gebremedhin Samson , Andargie Gashaw , Tsegaye Dawit A. , Alemayehu Wondwossen A. , Mekuria Legese Alemayehu , Wondie Tamiru , Fenta Teferi Gedif
Published: October 2023

Background

Vaccination is one of the most cost-effective public health interventions that prevents millions of deaths. Although immunization coverage is increasing globally, many children in low- and middle-income countries drop out of the vaccination continuum. This study aimed at determining vaccination dropout rates and predictors in children aged 12–35 months in remote and underserved areas of Ethiopia.

Methods

This study was part of a cross-sectional evaluation survey that was conducted in 2022 in Ethiopia. The study settings include pastoralist, developing & newly established regions, conflict affected areas, urban slums, internally displaced populations and refugees. A sample of 3,646 children aged 12–35 months were selected using a cluster sampling approach. Vaccination dropout was estimated as the proportion of children who did not get the subsequent vaccine among those who received the first vaccine. A generalized estimating equation was used to assess determinants of the dropout rate and findings were presented using an adjusted odds ratio with 95% confidence interval. Concentration curve and index were used to estimate wealth related inequality of vaccination dropout.

Results

A total of 3,646 caregivers of children participated in the study with a response rate of 97.7%. The BCG to Penta-3 (52.5%), BCG to MCV-2 (57.4%), and Penta-1 to Penta-3 (43.9%) dropouts were all high. The highest Penta-1 to Penta-3 dropout rate was found in developing regions (60.1%) and the lowest was in urban slums (11.2%). Caregivers who were working outside their homes [AOR (95% CI) = 3.67 (1.24–10.86)], who had no postnatal care follow-up visits [AOR (95%CI) = 1.66 (1.15–2.39)], who did not receive a service from a skilled birth attendant [AOR (95%CI) = 1.64 (1.21–2.27)], who were older than 45 years [AOR (95% CI) = 12.49 (3.87–40.33)], and who were less gender empowered [AOR (95%CI) = 1.63 (1.24–2.15)] had increased odds of Penta-1 to Penta-3 dropout. The odds of dropout for children from poor caregivers was nearly two times higher compared to their wealthy counterparts [AOR (95%CI) = 1.87 (1.38–2.52)].

Conclusion

Vaccination dropout estimates were high among children residing in remote and underserved settings. Poor wealth quintile, advanced maternal age, low women empowerment, and limited utilization of maternity care services contributed to vaccination dropout.
Efficacy of dual intracerebroventricular and intravitreal CLN5 gene therapy in sheep prompts the first clinical trial to treat CLN5 Batten disease
Authors: Murray Samantha J. , Wellby Martin P. , Barrell Graham K. , Russell Katharina N. , Deane Ashley R. , Wynyard John R. , Gray Steven J. , Palmer David N. , Mitchell Nadia L.
Published: October 2023

Mutations in the CLN5 gene cause the fatal, pediatric, neurodegenerative disease CLN5 neuronal ceroid lipofuscinosis. Affected children suffer progressive neuronal loss, visual failure and premature death. Presently there is no treatment. This study evaluated dual intracerebroventricular (ICV) and intravitreal (IVT) administration of a self-complementary adeno-associated viral vector encoding ovine CLN5 (scAAV9/oCLN5) into CLN5 affected sheep (CLN5−/−) at various disease stages. CLN5 disease progression was slowed in pre-symptomatic sheep who received a moderate dose of scAAV9/oCLN5, whilst a higher ICV dose treatment in early and advanced symptomatic animals delayed or halted disease progression. Intracranial (brain) volume loss was attenuated in all treatment cohorts, and visual function was also sustained in both the early and advanced symptomatic treated sheep over the 24-month duration of the study. Robust CLN5 protein expression was detected throughout the brain and spinal cord, and improvements in central nervous system and retinal disease correlates were observed. These findings hold translational promise for extending and improving the quality of life in both pre-symptomatic and symptomatic CLN5 patients, and prompted the initiation of the first in-human Phase I/II clinical trial testing ICV/IVT administration of scAAV9 encoding human CLN5 (https://clinicaltrials.gov/; NCT05228145).
Anaplastic lymphoma kinase inhibitors—a review of anticancer properties, clinical efficacy, and resistance mechanisms
Authors: Kiełbowski Kajetan , Żychowska Justyna , Becht Rafał
Published: October 2023

Fusions and mutations of anaplastic lymphoma kinase (ALK), a tyrosine kinase receptor, have been identified in several neoplastic diseases. Rearranged ALK is a driver of tumorigenesis, which activates various signaling pathway associated with proliferation and survival. To date, several agents that target and inhibit ALK have been developed. The most studied ALK-positive disease is non-small cell lung cancer, and three generations of ALK tyrosine kinase inhibitors (TKIs) have been approved for the treatment of metastatic disease. Nevertheless, the use of ALK-TKIs is associated with acquired resistance (resistance mutations, bypass signaling), which leads to disease progression and may require a substitution or introduction of other treatment agents. Understanding of the complex nature and network of resistance mutations may allow to introduce sequential and targeted therapies. In this review, we aim to summarize the efficacy and safety profile of ALK inhibitors, describe off-target anticancer effects, and discuss resistance mechanisms in the context of personalized oncology.
Treadmill training does not enhance skeletal muscle recovery following disuse atrophy in older male mice
Authors: Yee Elena M. , Hauser Carson T. , Petrocelli Jonathan J. , de Hart Naomi M. M. P. , Ferrara Patrick J. , Bombyck Princess , Fennel Zachary J. , van Onselen Lisha , Mookerjee Sohom , Funai Katsuhiko , Symons J. David , Drummond Micah J.
Published: October 2023

Introduction: A hallmark of aging is poor muscle recovery following disuse atrophy. Efficacious strategies to enhance muscle recovery following disuse atrophy in aging are non-existent. Prior exercise training could result in favorable muscle morphological and cellular adaptations that may promote muscle recovery in aging. Here, we characterized the impact of exercise training on skeletal muscle inflammatory and metabolic profiles and cellular remodeling and function, together with femoral artery reactivity prior to and following recovery from disuse atrophy in aged male mice. We hypothesized that 12 weeks of treadmill training in aged male mice would improve skeletal muscle cellular remodeling at baseline and during recovery from disuse atrophy, resulting in improved muscle regrowth.

Methods: Physical performance, ex vivo muscle and vascular function, tissue and organ mass, hindlimb muscle cellular remodeling (macrophage, satellite cell, capillary, myofiber size, and fibrosis), and proteolytic, inflammatory, and metabolic muscle transcripts were evaluated in aged exercise-trained and sedentary mice.

Results: We found that at baseline following exercise training (vs. sedentary mice), exercise capacity and physical function increased, fat mass decreased, and endothelial function improved. However, exercise training did not alter tibialis anterior or gastrocnemius muscle transcriptional profile, macrophage, satellite cell, capillarity or collagen content, or myofiber size and only tended to increase tibialis mass during recovery from disuse atrophy.

Conclusion: While exercise training in old male mice improved endothelial function, physical performance, and whole-body tissue composition as anticipated, 12 weeks of treadmill training had limited impact on skeletal muscle remodeling at baseline or in response to recovery following disuse atrophy.
Integration of comparative transcriptomics and WGCNA characterizes the regulation of anthocyanin biosynthesis in mung bean (Vigna radiata L.)
Authors: Li Chunxia , Gao Zexiang , Hu Weili , Zhu Xu , Li Youjun , Li Na , Ma Chao
Published: October 2023

Mung bean is a dual-use crop widely cultivated in Southeast Asia as a food and medicine resource. The development of new functional mung bean varieties demands identifying new genes regulating anthocyanidin synthesis and investigating their molecular mechanism. In this study, we used high-throughput sequencing technology to generate transcriptome sequence of leaves, petioles, and hypocotyls for investigating the anthocyanins accumulation in common mung bean variety as well as anthocyanidin rich mung bean variety, and to elucidate their molecular mechanisms. 29 kinds of anthocyanin compounds were identified. Most of the anthocyanin components contents were significantly higher in ZL23 compare with AL12. Transcriptome analysis suggested that a total of 93 structural genes encoding the anthocyanin biosynthetic pathway and 273 regulatory genes encoding the ternary complex of MYB-bHLH-WD40 were identified, of which 26 and 78 were differentially expressed in the two varieties. Weighted gene co-expression network analysis revealed that VrMYB3 and VrMYB90 might have enhanced mung bean anthocyanin content by inducing the expression of structural genes such as PAL, 4CL, F3’5’H, LDOX, and F3’H, which was consistent with qRT-PCR results. These findings are envisaged to provide a reference for studying the molecular mechanism of anthocyanin accumulation in mung beans.
Symbiotic compatibility between rice cultivars and arbuscular mycorrhizal fungi genotypes affects rice growth and mycorrhiza-induced resistance
Authors: Guigard Ludivine , Jobert Lea , Busset Nicolas , Moulin Lionel , Czernic Pierre
Published: October 2023

Introduction

Arbuscular mycorrhizal fungi (AMF) belong to the Glomeromycota clade and can form root symbioses with 80% of Angiosperms, including crops species such as wheat, maize and rice. By increasing nutrient availability, uptake and soil anchoring of plants, AMF can improve plant’s growth and tolerance to abiotic stresses. AMF can also reduce symptoms and pathogen load on infected plants, both locally and systemically, through a phenomenon called mycorrhiza induced resistance (MIR). There is scarce information on rice mycorrhization, despite the high potential of this symbiosis in a context of sustainable water management in rice production systems.

Methods

We studied the symbiotic compatibility (global mycorrhization & arbuscules intensity) and MIR phenotypes between six rice cultivars from two subspecies (indica: IR64 & Phka Rumduol; japonica: Nipponbare, Kitaake, Azucena & Zhonghua 11) and three AMF genotypes (Funneliformis mosseae FR140 (FM), Rhizophagus irregularis DAOM197198 (RIR) & R. intraradices FR121 (RIN)). The impact of mycorrhization on rice growth and defence response to Xanthomonas oryzae pv oryzae (Xoo) infection was recorded via both phenotypic indexes and rice marker gene expression studies.

Results

All three AMF genotypes colonise the roots of all rice varieties, with clear differences in efficiency depending on the combination under study (from 27% to 84% for Phka Rumduol-RIN and Nipponbare-RIR combinations, respectively). Mycorrhization significantly (α=0.05) induced negative to beneficial effects on rice growth (impact on dry weight ranging from -21% to 227% on Azucena-FM and Kitaake-RIN combinations, respectively), and neutral to beneficial effects on the extent of Xoo symptoms on leaves (except for Azucena-RIN combination which showed a 68% increase of chlorosis). R. irregularis DAOM197198 was the most compatible AMF partner of rice, with high root colonisation intensity (84% of Nipponbare’s roots hyphal colonisation), beneficial effects on rice growth (dry weight +28% (IR64) to +178% (Kitaake)) and decrease of Xoo-induced symptoms (-6% (Nipponbare) to -27% (IR64)). Transcriptomic analyses by RT-qPCR on leaves of two rice cultivars contrasting in their association with AMF show two different patterns of response on several physiological marker genes.

Discussion

Overall, the symbiotic compatibility between rice cultivars and AMF demonstrates adequate colonization, effectively restricting the nutrient starvation response and mitigating symptoms of phytopathogenic infection.
Hula as a physical activity and social support intervention for sustained activity in female breast and gynecologic cancer survivors
Authors: Bantum Erin O. , Yamada Paulette M. , Makolo TeMoana , Yu Herbert , Pagano Ian , Subia Natalie , Walsh Catherine , Loo Lenora W. M.
Published: October 2023

Background

Physical activity improves health and psychosocial functioning for people who have been diagnosed with cancer. Native Hawaiians face disparities for some cancers, including breast cancer. Delivering culturally grounded interventions has the potential to improve enjoyment and adherence to the intervention. We sought to test the adherence and impact of a 6 month randomized wait-list controlled trial of hula.

Methods

In this randomized wait-list controlled design people who had been diagnosed with breast or gynecologic cancers were invited to participate with other cancer survivors in a group based setting. Participants were randomized to begin hula immediately or after six months. Attendance was collected and heart-rate measured three times per session. In addition, demographic data, self-report psychosocial data, and biological data (findings will be reported elsewhere) were collected at three time points: baseline, 6 months, and 12 months. The study included six months of hula, twice per week, 60 min each session. In addition, participants committed to practice 60 min per week at home.

Results

Participants in the study (n = 42) attended, on average, 72% of the sessions. Significant increase in moderate physical activity (d = 0.50, p = 0.03) was observed in the intervention versus control group. For the measures of intra-individual changes pre-and post-intervention, an increase in total physical activity were seen in the intervention group (d = 0.69, p = 0.003), daily caloric intake decreased (d = −0.62, p = 0.007), and a reduction in waist circumference (d = −0.89, p = 0.0002) that was sustained six months after completion of the intervention. Psychosocially, cognitive functioning significantly declined from baseline to 12 months (d = −0.50, p = 0.03), with role functioning improving (d = 0.55, p = 0.02), social constraints increasing (d = 0.49, p = 0.03), and financial difficulties improving (d = −0.55, p = 0.02).

Conclusion

Sustainable physical activity is crucial to improve both the survival and quality of life of cancer survivors. Culturally grounded interventions, such as hula have the potential to increase the maintenance of physical activity. In addition, they create a support group where the benefits of people who have all experienced cancer can gather and garner those benefits of social support, too. This study was registered as a clinical trial through the National Cancer Institute (NCT02351479).

Clinical trial registration

Clinicaltrails.gov, NCT02351479.
Alcohol and psychoactive substance use in a cohort of children followed by child protection in France
Authors: Essadek Aziz , Musso Maeva , Assous Adèle , Widart Frédéric , Mathieu Joris , Robin Marion , Shadili Gérard
Published: October 2023

Background and aim

Many studies have investigated the association between maltreatment and substance use in adulthood.

In this study, we sought to explore the association between substance use during adolescence, diverse forms of child maltreatment, and psychological symptoms within a cohort of individuals under the purview of child protection services in France.

Method

The dataset was culled from a retrospective, population-based study encompassing minors and young adults aged 0 to 21, who were under the care of child protection services. Specifically, we conducted a comparative analysis between minors exhibiting substance use (N = 72) and those without such use (N = 776).

Result

The odds ratios predominantly illuminated a significant correlation between Substance Use and the manifestation of self-destructive behavior (OR = 4.35; CI 2.02–9.59), as well as aggressive behavior (OR = 5.75; CI 2.87–11.84). Univariate analysis also hinted at an association between SUD and suicidal ideation (OR = 3.52; CI 2.1–5.90).

Conclusion

Children in France who are in the care of child protection services and who use psychoactive substances are at greater risk of dropping out of school and of having other psychological symptoms. It is important that the public authorities take account of these results in order to adjust the care given to these minors, who often do not receive psychological support.
A bibliometric analysis of insomnia in adolescent
Authors: Gao Tianci , Tao Yulei , Wang Qianfei , Liu Jiayi , Du Zekun , Xing YueYi , Chen Fenqiao , Mei Jianqiang
Published: October 2023

Background

The negative effects of insomnia on adolescents’ development, academic performance, and quality of life place a burden on families, schools, and society. As one of the most important research directions for insomnia, adolescent insomnia has significant research value, social value, and practical significance. Unfortunately, there is no bibliometric analysis in this field of study. This study aims to analyze published articles using bibliometrics, summarize the current research progress and hot topics in this field systematically and exhaustively, and predict the future direction and trend of research.

Methods

For this study, the Web of Science Core Collection (WoSCC) database was searched between 2002 and 2022 for publications related to adolescent insomnia. The R–bibliometrix, VOSViewer, and CiteSpace software were utilized for bibliometric analysis.

Results

This investigation included 2468 publications from 3102 institutions in 87 countries, led by China and the United States. This field of research has entered a period of rapid development since 2017. The journal with the most publications on adolescent insomnia is Sleep, which is also the most co–cited journal. American Journal of Psychology has the highest impact factor among the top 10 journals. These papers were written by 10605 authors; notably, Liu Xianchen emerged as the author with the highest frequency of publications, while Mary A. Carskadon was the most frequently co–cited author. Mental health and comorbid diseases were the main research directions in this field. “Depression,” “anxiety,” “mental health,” “COVID–19,” “stress,” “quality of life,” “heart rate variability,” and “attention–deficit hyperactivity disorder” were hot spots and trends in this field at the current moment.

Conclusion

The research on adolescent insomnia has social value, research value, and research potential; its development is accelerating, and an increasing number of researchers are focusing on it. This study summarized and analyzed the development process, hot spots, and trends of adolescent insomnia research using bibliometric analysis, which identified the current hot topics in this field and predicted the development trend for the future.
Tourism experiences reduce the risk of cognitive impairment in the Chinese older adult: a prospective cohort study
Authors: Li Qian , Guo Zheng , Hu Fangli , Xiao Mengfei , Zhang Qiang , Wen Jun , Ying Tianyu , Zheng Danni , Wang Youxin , Yang Song , Hou Haifeng
Published: October 2023

Background

Given the etiological complexity of cognitive impairment, no effective cure currently exists for precise treatment of dementia. Although scholars have noted tourism’s potential role in managing cognitive impairment and mild dementia, more robust empirical investigation is needed in this area. This study aimed to examine the associations between tourism and cognitive impairment and dementia in older Chinese adults.

Method

From a nationwide community-based cohort, 6,717 individuals aged ≥60 were recruited from 2011 to 2014, of whom 669 (9.96%) had had at least one tourism experience in the 2 years prior to enrollment. All the participants were then prospectively followed up until 2018. The association between tourism and cognitive impairment was examined by the Cox proportional hazards regression model. The adjusted hazard ratio (aHR) and its 95% confidence interval (CI) were calculated to evaluate the effect of tourism experience on cognitive impairment and dementia.

Results

A total of 1,416 individuals were newly diagnosed with cognitive impairment and 139 individuals with dementia onset during follow-up. The incidence of cognitive impairment was significantly lower among participants with tourism experiences (316.94 per 10,000 person-years) than those without such experiences (552.38 per 10,000 person-years). Cox regression showed that tourism decreased the risk of cognitive impairment (aHR = 0.69, 95% CI: 0.41–0.62) when adjusted for behavioral covariates and characteristics. Compared with participants without tourism experiences, those with 1, 2, and ≥3 tourism experiences had a lower risk of cognitive impairment with the aHRs of 0.72 (95% CI: 0.52–0.99), 0.65 (0.42–1.01), and 0.68 (0.44–0.98), respectively. Tourism experiences also reduced participants’ risk of dementia (aHR = 0.41, 95% CI: 0.19–0.89).

Conclusion

Our findings demonstrated associations between tourism and reduced risks of cognitive impairment and dementia in older Chinese adults. Thus, tourism could serve as a novel approach to dementia prevention.
Potential benefits of yeast Saccharomyces and their derivatives in dogs and cats: a review
Authors: Maturana Marta , Castillejos Lorena , Martin-Orue Susana M. , Minel Anaelle , Chetty Olivia , Felix Ananda P. , Adib Lesaux Achraf
Published: October 2023

Yeast Saccharomyces and its derivatives have been largely used in livestock and poultry nutrition for their potential positive impact on growth, performance, and general health. Originally included in animal diets as a source of protein, yeasts can also offer a wide range of by-products with interesting bioactive compounds that would confer uses beyond nutrition. Although its supplementation in livestock, poultry and even in humans is well documented, the available body of literature on the use of yeast and its derivatives in companion animals’ food, mainly dogs and cats’ diets, is still developing. Despite this, gut microbiota modulation, immune system enhancement or decreasing of potentially pathogenic microorganisms have been reported in pets when using these products, highlighting their possible role as probiotics, prebiotics, and postbiotics. This review attempts to provide the reader with a comprehensive on the effects of Saccharomyces and its derivatives in pets and the possible mechanisms that confer their functional properties.
Research Trends in Molecular Biological Studies on Oral Squamous Cell Carcinoma: A Bibliometric Analysis
Authors: Gunardi Indrayadi , Sufiawati Irna , Goenawan Hanna , Herawati Dewi Marhaeni Diah , Lesmana Ronny , Abdullah Ade Gafar
Published: October 2023

Background: Since the discovery of PCR and ELISA, in vitro research in the realm of molecular biology pertaining to oral squamous cell carcinoma (OSCC) has witnessed significant expansion.

Objective: to provide a comprehensive overview of molecular biology research on OSCC through visual mapping techniques.

Methods: We conducted an analysis of publications within the “oral squamous cell carcinoma” category from Scopus’ core collection. On 20 January 2023, we screened these publications using an advanced search employing the keywords “oral squamous cell cancer” and “cell line.” Data analysis was performed using Microsoft Excel 2010 and VOSviewer, facilitating the examination of author contributions, journal productivity, institutional affiliations, and contributions by nations. VOSviewer was further utilized for co-occurrence and reference analysis of keywords.

Results: A total of 781 papers spanning from 1992 to 2023 were collected. Notably, Japan, China, and the United States emerged as significant contributors in this field. The Osaka University Graduate School of Dentistry (Japan) ranked first with 21 publications. Chae J-I of Chonbuk National University (South Korea) emerged as the most prolific author, with 14 publications. The International Journal of Oncology and the Journal of Oral Pathology and Medicine were identified as the two most prolific journals. The central themes that emerged were epidermal growth factor receptor, invasion, epithelial-mesenchymal transition, angiogenesis, apoptosis, and metastasis.

Conclusion: The rate of publications focused on the molecular biology of OSCC has seen a remarkable increase. Research priorities have shifted from topics such as “radiation, RANKL, cyclin D1, RNA interference, and matrix metalloproteinase” to encompass areas such as “chemoresistance due to cisplatin, other therapeutic agents (metformin and monoclonal antibody), autophagy, inflammation, microRNA, cancer-associated fibroblasts, and STAT3 (with roles in cell migration and tumorigenesis).” These seven significant future research areas hold promise in identifying reliable biological markers for oral cancer detection and treatment, thereby improving clinical outcomes.
Analytical Formulations for Nitrogen Oxides Emissions Estimation of an Air Turbo-Rocket Engine Using Hydrogen
Authors: Viola Nicole , Fusaro Roberta , Saccone Guido , Borio Valeria
Published: October 2023

According to the latest report of the Intergovernmental Panel on climate change, aviation contributes to only about 2% to anthropogenic global greenhouse gas (GHG) emissions. However, in view of the growing market demand and the dramatic reductions in other transport sectors, including maritime and automotive, the aviation sector’s percentage impact on global GHG emissions is expected to reach 50% of the transport share by 2040. High-speed aviation exploiting liquid hydrogen as the propellant can represent a valuable solution toward the decarbonization of the sector. However, to avoid jeopardizing the dream of a new generation of high-speed aircraft, it will be necessary to introduce non-CO2 emissions estimations beginning with the design process. To unlock the possibility of anticipating the nitrogen oxides emissions estimation, the authors developed the hydrogen and high-speed P3-T3 methodology (H2-P3T3), an evolution of the widely used P3-T3 method, properly conceived to support (i) innovative air-breathing propulsive systems for supersonic and hypersonic flights and (ii) greener fuels, such as hydrogen. This paper presents a step-by-step approach to developing novel analytical formulations customized for an Air Turbo-Rocket engine and discusses the discovered correlation of nitrogen oxides production with the fuel-to-air ratio (FAR), the Mach number, and the Damköhler number (Da), the last being a nondimensional variable directly related to hydrogen/air combustion, considering the matching between the residence time and the ignition delay times. The most complete formulation allows for reduction in the prediction errors below 5%.
Modeling of Path Loss for Radio Wave Propagation in Wireless Sensor Networks in Cassava Crops Using Machine Learning
Authors: Barrios-Ulloa Alexis , Cama-Pinto Alejandro , De-la-Hoz-Franco Emiro , Ramírez-Velarde Raúl , Cama-Pinto Dora
Published: October 2023

Modeling radio signal propagation remains one of the most critical tasks in the planning of wireless communication systems, including wireless sensor networks (WSN). Despite the existence of a considerable number of propagation models, the studies aimed at characterizing the attenuation in the wireless channel are still numerous and relevant. These studies are used in the design and planning of wireless networks deployed in various environments, including those with abundant vegetation. This paper analyzes the performance of three vegetation propagation models, ITU-R, FITU-R, and COST-235, and compares them with path loss measurements conducted in a cassava field in Sincelejo, Colombia. Additionally, we applied four machine learning techniques: linear regression (LR), k-nearest neighbors (K-NN), support vector machine (SVM), and random forest (RF), aiming to enhance prediction accuracy levels. The results show that vegetation models based on traditional approaches are not able to adequately characterize attenuation, while models obtained by machine learning using RF, K-NN, and SVM can predict path loss in cassava with RMSE and MAE values below 5 dB.
Long-Term Application of Manure and Different Mineral Fertilization in Relation to the Soil Organic Matter Quality of Luvisols
Authors: Balík Jiří , Suran Pavel , Sedlář Ondřej , Černý Jindřich , Kulhánek Martin , Procházková Simona , Asrade Dinkayehu Alamnie , Smatanová Michaela
Published: October 2023

Long-term field experiments were conducted on luvisol at five sites in the Czech Republic (42–48-year duration). The average total organic carbon content in the soil varied between 9.0 and 14.0 g kg−1. In these trials, seven crops were rotated in the following order: clover, winter wheat, early potato, winter wheat, spring barley, potato, and spring barley with interseeded clover. Five treatments were studied: unfertilized treatment (Con), farmyard manure (F), and combinations of farmyard manure with three mineral fertilization levels (F+M1, F+M2, F+M3). Plant residues were not incorporated into the soil. An amount of 40 t ha−1 of farmyard manure fresh matter was applied twice during crop rotation. Intensive mineral fertilizer (F+M3) increased the average value of the carbon sequestration efficiency (CSE) by 12.9% and up to 26.3%. Combining organic and mineral fertilizers at moderate and higher intensities increased the soil organic matter quantity and quality compared to the unfertilized or manure treatment. Data on the glomalin content can be used to study the organic matter quality. We determined a strong correlation between the total glomalin content and the soil organic matter carbon, fulvic acid content, humic acid content, extractable carbon content, and dissolved organic carbon content, as well as the potential wettability index and aromaticity index.
Increased Accumulation of Recombinant Proteins in Soybean Seeds via the Combination Strategy of Polypeptide Fusion and Suppression of Endogenous Storage Proteins
Authors: Yang Jing , Zhang Yuanyu , Xing Guojie , Wei Jia , Niu Lu , Zhao Qianqian , Cai Qinan , Zhong Xiaofang , Yang Xiangdong
Published: October 2023

Soybean seeds show great potential as a safe and cost-effective host for the large-scale production of biopharmaceuticals and industrially important macromolecules. However, the yields of desired recombinant proteins in soybean seeds are usually lower than the economic threshold for their potential commercialization. Our previous study demonstrated that polypeptide fusion such as maize γ-zein or elastin-like polypeptide (ELP) could significantly increase the accumulation of foreign proteins. In the present study, a recombination strategy of polypeptide fusions (γ-zein or ELP) and suppression of intrinsic storage proteins (glycinin or conglycinin) via RNA interference was further exploited to improve the yield of the target protein in soybean seeds. Transgenic soybean plants harboring both polypeptide-fused green fluorescent protein (GFP) and glycinin/conglycinin RNAi expression cassettes were generated and confirmed by molecular analysis. The results showed that on both the glycinin and conglycinin suppression backgrounds, the average accumulation levels of recombinant zein-GFP and GFP-ELP proteins were significantly increased as compared to that of their counterparts without such suppressions in our previous study. Moreover, zein-GFP and GFP-ELP accumulation was also remarkably higher than unfused GFP on the glycinin suppression background. However, no significant differences were detected in the glycinin or conglycinin suppression backgrounds for the same polypeptide fusion constructs, though suppression of one of the storage proteins in soybean seeds led to a significant increase in the other. Additionally, the increases in the recombinant protein yield did not affect the total protein content and the protein/oil ratio in soybean seeds. Taken together, the results indicate that both the fusion of the foreign protein with polypeptide tags together with the depletion of endogenous storage proteins contributed to a higher accumulation of the recombinant proteins without affecting the total protein content or the protein/oil ratio in soybean seeds.
Sequential Acid/Alkali Pretreatment for an Olive Tree Pruning Biorefinery
Authors: Díaz Manuel J. , Ferrero Pedro M. , Moya Manuel
Published: October 2023

Olive tree pruning is an abundant and renewable lignocellulosic residue, which is generally burned in the fields, causing economic costs and environmental problems. This lignocellulosic residue can be considered a suitable raw material for the production of a wide range of byproducts in a biorefinery context due to its high content of potentially fermentable carbohydrates. To take advantage of its sugar content, pretreatment is necessary to enhance the accessibility of the enzymes to the cellulosic fraction. The aim of this work is to obtain sugars contained in olive tree pruning as a substrate for the production of bioethanol by fermentation. Specifically, the production of fermentable sugars by sequential pretreatment with sulfuric acid and sodium hydroxide is studied. A two-factor rotatable composite central design temperature and catalyst concentration (H2SO4 and NaOH) has been generated, and response surface methodology has been used to discuss and optimize the responses. This work shows that under optimal pretreatment conditions (130 °C, 1.90% w/v H2SO4 and 130 °C, 1.49% w/v NaOH) of 1 kg of olive tree pruning, a solution rich in sugars (102 g of glucose and 61 g of xylose) and a solid residue generating 99 g of glucose by enzymatic hydrolysis is obtained. Moreover, applying the combined severity to the acid pretreatment, it has been determined that 20% of the olive tree pruning is fast solubilization, and it was also found that the apparent activation energy of the acid hydrolysis reaction is 85.07 kJ/mol.
Collision Avoidance for a Selective Compliance Assembly Robot Arm Manipulator Using Topological Path Planning
Authors: Batista Josias G. , Ramalho Geraldo L. B. , Torres Marcelo A. , Oliveira Anderson L. , Ferreira Daniel S.
Published: October 2023

Industrial applications with robotic manipulators have grown and made production systems increasingly efficient. However, there are still some limitations that can delay production, causing losses. Several factors, such as accidents and collisions of manipulator robots with operators and other machines, can cause unforeseen stops. Thus, this work aims to develop a trajectory planning method to avoid collisions applied to a selective compliance assembly robot arm (SCARA) robotic manipulator in the context of collaborative robotics. The main contribution of this paper is a path planning method based on mathematical morphology, named topological path planning (TPP). Through some evaluation metrics such as the number of path points, computing time, distance, standard deviation of the joint acceleration, and maximum acceleration rate along the path, we show that TPP is a collision-free, deterministic, and predictable route planning. In our experiments, our proposal presented better results for applications in industrial robotic manipulators when compared to the probabilistic roadmap method (PRM) and TPP*, a particular case of TPP that is similar to the generalized Voronoi diagram (GVD).