bluuebunny/crossref_metadata_2025 huggingface dataset:
Undergraduate Students’ Definitions of Sexual Violence Labels Across the Rise of the #MeToo Movement
Authors: Kilimnik Chelsea D. , McMahon Leah N. , Meston Cindy M.
Published: March 2024

Examining changes in perspectives of sexual violence across the #MeToo movement is critical to understanding shifts in sociocultural contexts of sexual violence; yet the literature is still nascent. In the present study, we assessed variation across the #MeToo movement in undergraduate students’ ( N = 1,028) rape myth acceptance, sexual violence experiences, identification of experiences with sexual violence labels, and self-generated definitions of sexual violence labels (e.g., sexual assault). We also examined the mediating role of the relation between timepoint across #MeToo and students’ self-generated sexual violence definitions. Text analyses revealed seven themes in students’ definitions: Activity, Chronicity, Body, Contact, Gender, Force/Harm, and Consent. Notably, there was greater representation and a higher rate of change in the Contact theme in definitions following the #MeToo movement compared to pre-#MeToo, but only among students with sexual violence history. Rape myth acceptance did not change significantly across the #MeToo movement and did not demonstrate a significant role in time-related variability in students’ definitions. These findings provide support for the role of the #MeToo movement in facilitating more inclusive perceptions of sexual violence among undergraduate students, especially among those with sexual violence histories. We hope that with increased insight into the #MeToo movement's impact on sexual violence perspectives, continued efforts of the movement and prevention initiatives can broaden their reach.
Power sharing with weak institutions
Authors: Powell Robert
Published: March 2024

Democratic transitions, franchise extensions, and civil war settlements can often be seen as power-sharing agreements in which opposing factions try to use institutional structures to ‘lock in’ the terms of a settlement. But the commitment power inherent in institutions varies. When the institutional environment is weak and credibility is low, it is difficult for a powerful elite to tie its hands and give up power. This article studies a window-of-opportunity model in which an enfranchised elite faces a periodic threat. Institutional weakness is parameterized in terms of the elite’s marginal return to trying to undermine a power-sharing agreement. The analysis shows that (i) bargaining breaks down if the overall institutional environment is too weak and why it does; (ii) equilibrium agreements share more power with the opposition when the institutional environment is weak; (iii) there is a non-monotonic relation between power sharing and how often the opposition poses a threat; and (iv) power sharing is path dependent.
Enhancing quality control in emulsion-type sausage production: Predicting chemical composition of intact samples with near infrared spectroscopy
Authors: Ritthiruangdej Pitiporn , Vangnai Kanithaporn , Kasemsumran Sumaporn , Somboonying Supapich , Charoensin Pimwaree , Hiriotappa Arisara , Lowleraha Papawarin
Published: March 2024

This research actively explores the potential of near infrared spectroscopy (NIR) for analyzing the chemical composition of emulsion-type sausages, focusing on critical factors like residual nitrite, moisture, protein, and fat content. To establish robust and generalizable models, we utilized a dataset of 100 experimentally prepared sausages encompassing a wide range of pork back fat replacement levels (5%, 15%, 30%, 45%, and 60%) and added sodium nitrite amounts (0, 80, 125, 250, and 375 ppm). An external validation set of 20 commercially sourced sausages further assessed the model’s real-world applicability. Partial least squares (PLS) regression calibration models with multiplicative scatter correction (MSC) pre-treatment demonstrated impressive accuracy for moisture (RMSECV = 0.57%, RPD = 9.8), fat (RMSECV = 1.17%, RPD = 9.5), and protein (RMSECV = 0.30%, RPD = 7.6). While residual nitrite prediction presented challenges due to its inherent complexity, the external validation yielded a competitive root mean square error of prediction (RMSEP) of 12.02 ppm, surpassing the average performance reported in similar studies (RMSEP ∼15 ppm) by 3 ppm. Importantly, sample homogenization did not significantly affect parameter prediction, highlighting the robustness of the NIR-based approach. These findings suggest that NIR spectroscopy, with its non-destructive, rapid, and cost-effective nature, could provide valuable tools for quality control and monitoring in the emulsion-type sausage industry. More importantly, improved nitrite prediction could pave the way for enhanced precision and control in sausage production, ultimately contributing to improved food safety and sustainability.
Exploring bending behavior of curved sandwich panels with three-dimensional printed, functionally graded cores
Authors: Farajollahi Amirhamzeh , Rostami Mohsen , Baharvand Mohammad , Chandra Subhash , Singh Bains Pardeep
Published: March 2024

Sandwich structures have garnered significant attention due to their high strength-to-weight ratio in various industries, particularly aerospace. Meeting application demands requires optimizing mechanical properties such as bending stiffness, peak load, specific absorbed energy, and weight. This study presents a unique approach involving the design and manufacturing techniques of curved sandwich panels with functionally graded cores, aiming to achieve a comprehensive spectrum of bending properties. Curved structures have applications across diverse fields, including landing gear. The semi-circular core of the sandwich panel comprised three distinct regions defined by angles: Ф, Υ, and 90-Ф- Υ. These angles specified both the location and proportion of different honeycomb cells, including high, medium, and low-density cells. Any variations in these angles and their cell types resulted in a new density gradient. The manufactured sandwich structures consisted of polylactic acid cores printed by a fused deposition modeling printer, sandwiched between aluminum skins. Experimental tests and finite element analysis for three models showed strong agreement, with a maximum error of 14.45%. After the simulation was validated, it expanded to cover other configurations. Subsequently, mathematical models based on the aforementioned angles were calibrated using results extracted from the simulation step. This process led to achieving various structures characterized by a wide range of stiffness (ranging from 0.29 to 0.79 kN/mm), peak load (ranging from 1.73 to 4.77 kN), and specific absorbed energy values (ranging from 41.78 to 96.09 J/kg). The proposed methodology exhibits promise in engineering the design of these structures and their multi-objective optimization.
Digital-Based Interventions for Complex Post-Traumatic Stress Disorder: A Systematic Literature Review
Authors: Blackie Meg , De Boer Kathleen , Seabrook Liz , Bates Glen , Nedeljkovic Maja
Published: March 2024

Research has shown that complex post-traumatic stress disorder (cPTSD) differs from post-traumatic stress disorder (PTSD) on core symptoms relating to the individual’s sense of self, and this has driven the need for treatment approaches to address these specific features of cPTSD. The COVID-19 pandemic has led to the increased use of digital-based interventions (DBIs) to treat mental illnesses, including trauma-related disorders. However, while evidence for the use of DBIs for PTSD has previously been synthesized, the current review is the first synthesis of research on the use of DBIs for cPTSD. A systematic search of Scopus, PsychINFO, and EBSCOhost was conducted, using search terms targeting “cPTSD” and “DBIs,” to identify research on the use of DBIs to treat cPTSD symptoms. Ten papers were identified, which provided preliminary evidence for the efficacy of DBIs to reduce cPTSD symptoms. Further, DBIs were reported as acceptable by individuals with a history of complex trauma. The paper also provides insight into the therapeutic approaches adopted, digital modalities utilized, safety measures included, and whether/to what degree support was provided. While DBIs show promise for treating cPTSD, there is substantial room for advancement of the empirical evidence base for these approaches. Both clinical and research-based recommendations are provided separately.
Emplacement and Emplotment: Media Production in Pandemic Times
Authors: Mayer Vicki , Lavie Noa
Published: March 2024

Places matter, despite the discursive hype of virtual making or remote locations, in showing the ways that production is a process nested within social worlds. In 2020, global Hollywood promoted a “COVID-friendly ideal” in order to return to work during widespread lockdowns and work stoppages. Yet by focusing comparatively on the stories that film and television workers told about production during COVID, we argue that crisis stories reveal much about the specificity of places in studying production cultures. To illustrate, we compare workers’ pandemic stories as evidence of emplacement in national hierarchies and emplotment in local power dynamics. Drawing on a corpus of seventy interviews conducted in Tel Aviv, Israel and New Orleans, USA in 2020 and 2021, we show that production cultures provide ample critique of normative tales that media industries promote about themselves and their workforces.
The Status of University Business Schools Regarding Industry 4.0: From the Turkish Perspective
Authors: Ilhan-Nas Tulay , Saglam Aysegul , Okan Tarhan , Peker Iskender
Published: March 2024

Industry 4.0, whose effects have been more and more noticeable in recent years, and the digital change it brings call for a new educational model that aligns university instructional processes and curricula with the demands of business. This approach, known as University 4.0, intends to promote more technology-based applications, the power of innovation, the training of skilled specialists to satisfy industrial needs, and the development of competences that can achieve multidisciplinary projects. It is of utmost importance to adapt late-industrializing nations’ educational systems, such as Turkey’s, to this change process. The aim of this research, which takes the Turkish context as a reference, is to reveal how the curricula and educational processes of business schools should be shaped with a perspective that takes into account the human resources requirements of industry 4.0. A decision-making technique that integrates QFD, Delphi, and DEMATEL methods was employed in the study. The results clearly show that the most important expectation for social stakeholders is to provide university-industry cooperation. However, some of the most pressing problems include applied learning through collaborative educational experiences, teamwork skills, changing roles and competencies of academics. The findings point out that the educational activities of business schools must be linked with industry expectations through technology-based training activities and teamwork.
Iguratimod, an allosteric inhibitor of macrophage migration inhibitory factor (MIF), prevents mortality and oxidative stress in a murine model of acetaminophen overdose
Authors: Bloom Joshua , Pantouris Georgios , He Mingzhu , Aljabari Bayan , Mishra Lopa , Manjula Ramu , Parkins Andrew , Lolis Elias J. , Al-Abed Yousef
Published: March 2024

Background

Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine that has been implicated in multiple inflammatory and non-inflammatory diseases, including liver injury induced by acetaminophen (APAP) overdose. Multiple small molecule inhibitors of MIF have been described, including the clinically available anti-rheumatic drug T-614 (iguratimod); however, this drug’s mode of inhibition has not been fully investigated.

Methods

We conducted in vitro testing including kinetic analysis and protein crystallography to elucidate the interactions between MIF and T-614. We also performed in vivo experiments testing the efficacy of T-614 in a murine model of acetaminophen toxicity. We analyzed survival in lethal APAP overdose with and without T-614 and using two different dosing schedules of T-614. We also examined MIF and MIF inhibition effects on hepatic hydrogen peroxide (H2O2) as a surrogate of oxidative stress in non-lethal APAP overdose.

Results

Kinetic analysis was consistent with a non-competitive type of inhibition and an inhibition constant (Ki) value of 16 µM. Crystallographic analysis revealed that T-614 binds outside of the tautomerase active site of the MIF trimer, with only the mesyl group of the molecule entering the active site pocket. T-614 improved survival in lethal APAP overdose when given prophylactically, but this protection was not observed when the drug was administered late (6 h after APAP). T-614 also decreased hepatic hydrogen peroxide concentrations during non-lethal APAP overdose in a MIF-dependent fashion.

Conclusions

T-614 is an allosteric inhibitor of MIF that prevented death and decreased hepatic hydrogen peroxide concentrations when given prophylactically in a murine model of acetaminophen overdose. Further studies are needed to elucidate the mechanistic role of MIF in APAP toxicity.
A transcriptome-wide identification of ATP-binding cassette (ABC) transporters revealed participation of ABCB subfamily in abiotic stress management of Glycyrrhiza glabra L
Authors: Devi Ritu , Goyal Pooja , Verma Bhawna , Hussain Shahnawaz , Chowdhary Fariha , Arora Palak , Gupta Suphla
Published: March 2024

Transcriptome-wide survey divulged a total of 181 ABC transporters in G. glabra which were phylogenetically classified into six subfamilies. Protein–Protein interactions revealed nine putative GgABCBs (-B6, -B14, -B15, -B25, -B26, -B31, -B40, -B42 &-B44) corresponding to five AtABCs orthologs (-B1, -B4, -B11, -B19, &-B21). Significant transcript accumulation of ABCB6 (31.8 folds), -B14 (147.5 folds), -B15 (17 folds), -B25 (19.7 folds), -B26 (18.31 folds), -B31 (61.89 folds), -B40 (1273 folds) and -B42 (51 folds) was observed under the influence of auxin. Auxin transport-specific inhibitor, N-1-naphthylphthalamic acid, showed its effectiveness only at higher (10 µM) concentration where it down regulated the expression of ABCBs, PINs (PIN FORMED) and TWD1 (TWISTED DWARF 1) genes in shoot tissues, while their expression was seen to enhance in the root tissues. Further, qRT-PCR analysis under various growth conditions (in-vitro, field and growth chamber), and subjected to abiotic stresses revealed differential expression implicating role of ABCBs in stress management. Seven of the nine genes were shown to be involved in the stress physiology of the plant. GgABCB6, 15, 25 and ABCB31 were induced in multiple stresses, while GgABCB26, 40 & 42 were exclusively triggered under drought stress. No study pertaining to the ABC transporters from G. glabra is available till date. The present investigation will give an insight to auxin transportation which has been found to be associated with plant growth architecture; the knowledge will help to understand the association between auxin transportation and plant responses under the influence of various conditions.
Bioinformatics integration reveals key genes associated with mitophagy in myocardial ischemia-reperfusion injury
Authors: Chen Zhian , Liu Tianying , Yuan Hao , Sun Han , Liu Sitong , Zhang Shuai , Liu Li , Jiang Shuang , Tang Yong , Liu Zhi
Published: March 2024

Background

Myocardial ischemia is a prevalent cardiovascular disorder associated with significant morbidity and mortality. While prompt restoration of blood flow is essential for improving patient outcomes, the subsequent reperfusion process can result in myocardial ischemia–reperfusion injury (MIRI). Mitophagy, a specialized autophagic mechanism, has consistently been implicated in various cardiovascular disorders. However, the specific connection between ischemia–reperfusion and mitophagy remains elusive. This study aims to elucidate and validate central mitophagy-related genes associated with MIRI through comprehensive bioinformatics analysis.

Methods

We acquired the microarray expression profile dataset (GSE108940) from the Gene Expression Omnibus (GEO) and identified differentially expressed genes (DEGs) using GEO2R. Subsequently, these DEGs were cross-referenced with the mitophagy database, and differential nucleotide sequence analysis was performed through enrichment analysis. Protein–protein interaction (PPI) network analysis was employed to identify hub genes, followed by clustering of these hub genes using cytoHubba and MCODE within Cytoscape software. Gene set enrichment analysis (GSEA) was conducted on central genes. Additionally, Western blotting, immunofluorescence, and quantitative polymerase chain reaction (qPCR) analyses were conducted to validate the expression patterns of pivotal genes in MIRI rat model and H9C2 cardiomyocytes.

Results

A total of 2719 DEGs and 61 mitophagy-DEGs were identified, followed by enrichment analyses and the construction of a PPI network. HSP90AA1, RPS27A, EEF2, EIF4A1, EIF2S1, HIF-1α, and BNIP3 emerged as the seven hub genes identified by cytoHubba and MCODE of Cytoscape software. Functional clustering analysis of HIF-1α and BNIP3 yielded a score of 9.647, as determined by Cytoscape (MCODE). In our MIRI rat model, Western blot and immunofluorescence analyses confirmed a significant elevation in the expression of HIF-1α and BNIP3, accompanied by a notable increase in the ratio of LC3II to LC3I. Subsequently, qPCR confirmed a significant upregulation of HIF-1α, BNIP3, and LC3 mRNA in the MIRI group. Activation of the HIF-1α/BNIP3 pathway mediates the regulation of the degree of Mitophagy, thereby effectively reducing apoptosis in rat H9C2 cardiomyocytes.

Conclusions

This study has identified seven central genes among mitophagy-related DEGs that may play a pivotal role in MIRI, suggesting a correlation between the HIF-1α/BNIP3 pathway of mitophagy and the pathogenesis of MIRI. The findings highlight the potential importance of mitophagy in MIRI and provide valuable insights into underlying mechanisms and potential therapeutic targets for further exploration in future studies.
Upregulation of CIRP by its agonist prevents the development of heart failure in myocardial infarction rats
Authors: Zhang Jingjing , Liu Tao , Wei Yanzhao , Peng Jianye , Zeng Gaofeng , Zhong Peng
Published: March 2024

Background

Downregulated expression of cold-inducible RNA binding protein (CIRP), a stress-response protein, has been demonstrated in the hearts of patients with heart failure (HF). However, whether CIRP plays a critical role in the pathogenesis of HF remains unknown. Zr17-2 is a recently identified CIRP agonist, which can enhance the expression of CIRP in hearts. Herein, we evaluated the effects of zr17-2 on the development of HF in a rat model of myocardial infarction (MI).

Methods

Male SD rats were pretreated with CIRP agonist zr17-2 or vehicle saline for 6 consecutive days, followed by MI induction. 1-week post-MI, cardiac function, and structural and molecular changes were determined by echocardiography and molecular biology methods.

Results

Excitingly, we found that pretreatment with zr17-2 significantly attenuated MI-induced cardiac dysfunction and dilation, coupled with reduced infarction size and cardiac remodeling. In addition, increased inflammatory response in the peri-infarcted heart including macrophage infiltration and the expression of inflammatory genes were all significantly decreased by zr17-2 pretreatment, suggesting an anti-inflammatory effect of zr17-2. Moreover, zr17-2 pretreatment also upregulated the antioxidant genes (e.g. NQO-1, Nrf2, and HO-1) level in the hearts. In isolated cultured cardiomyocytes, pretreatment with zr17-2 markedly attenuated cell injury and apoptosis induced by oxidative injury, along with elevation of Nrf2-related antioxidant genes and CIRP. However, silencing CIRP abolished zr17-2’s antioxidant effects against oxidative injury, confirming that zr17-2’s role is dependent on CIRP.

Conclusion

Collectively, our study suggests CIRP plays a crucial role in the development of HF and a beneficial effect of CIRP agonist in preventing MI-induced HF, possibly via anti-inflammatory and anti-oxidant pathways.
Food insecurity in urban American Indian and Alaska Native populations during the COVID-19 pandemic
Authors: Nelson Katie , Jackson Alexandra M. , Nguyen Cassandra J. , Noonan Carolyn , Muller Clemma , MacLehose Richard F. , Manson Spero M. , Dillard Denise A. , Buchwald Dedra ,   CONCERTS Collaborative, Madesclaire Odile , Henderson Austin , Quandelacey Talia
Published: March 2024

Background

Food insecurity is an important social determinant of health that was exacerbated by the COVID-19 pandemic. Both food insecurity and COVID-19 infection disproportionately affect racial and ethnic minority groups, particularly American Indian and Alaska Native communities; however, there is little evidence as to whether food insecurity is associated with COVID-19 infection or COVID-19 preventive behaviors such as vaccination uptake. The purpose of this study was to evaluate associations between food insecurity, COVID-19 infection, and vaccination status among urban American Indian and Alaska Native adults seen at 5 clinics serving urban Native people.

Methods

In partnership with health organizations in Alaska, Colorado, Kansas, Minnesota, and New Mexico, the study team conducted a cross-sectional survey in 2021 to assess food security status and attitudes, barriers, and facilitators for COVID-19 testing and vaccination. Logistic regression was used to examine the association of food security status with sociodemographic factors and COVID-19 infection and vaccination status. Marginal standardization was applied to present results as prevalence differences.

Results

Among 730 American Indian and Alaska Native adults, the prevalence of food insecurity measured during the pandemic was 38%. For participants who reported persistent food security status before and during the pandemic (n = 588), the prevalence of food insecurity was 25%. Prevalence of COVID-19 infection and vaccination did not vary by food security status after adjustment for confounders.

Conclusions

High rates of food insecurity among American Indian and Alaska Native communities likely increased during the COVID-19 pandemic. However, despite the high prevalence of food insecurity, community-led efforts to reduce COVID-19 infection and increase vaccination uptake across Indian Health Service and Tribal healthcare facilities may have mitigated the negative impacts of the pandemic for families experiencing food insecurity. These successful approaches serve as an important reference for future public health efforts that require innovative strategies to improve overall health in American Indian and Alaska Native communities.
The individuals’ awareness and adoption of electronic health records in China: a questionnaire survey of 1,337 individuals
Authors: Xu Yizhou , Pei Zongmin , He Xing , Guo Lu , Zeng Li , Huang Xiaoxuan , Zhang Jian
Published: March 2024

Background

Electronic health records (EHRs) are digital records of individual health information. However, their adoption and utilization remain low. This study explores the factors influencing the implementation of EHRs through a questionnaire survey to enhance individual awareness and adoption of EHRs.

Methods

A questionnaire and an expert rating scale were developed sequentially, and the consistency of the scores from five experts was calculated using Kendall’s W to generate a final questionnaire. A non-parametric test was utilized to analyze differences in continuous data that did not follow a normal distribution. Categorical variables were expressed as percentages (%), the chi-square test was employed for group comparisons, and multiple logistic regression was implemented to assess individuals’ awareness and adoption of EHRs.

Results

In total, 1,341 survey questionnaires were distributed between January and December 2022, with 1,337 valid responses (99.7%). The results indicated that the proportion of participants who were aware of EHRs and had a bachelor’s degree or higher education, an income of ≥$700 per month, residence in urban areas, possessed self-care abilities, and underwent annual physical examinations was significantly higher than that without awareness of EHRs (P < 0.05), while in hearing problems and walking abilities was markedly lower than that of participants without awareness of EHRs (P < 0.05). Additionally, the proportion of individuals willing to self-manage EHRs was significantly higher than those reluctant to do so (P < 0.05) among participants with a bachelor’s degree or higher education, an income of ≥$700 per month, residence in urban areas, possession of self-care abilities, annual physical examinations, hearing problems, and poor walking abilities. Age (Odds Ratio [OR] = 1.104, 95% Confidence Interval [CI] 1.001–1.028, P = 0.033), hearing problems (OR = 0.604, 95% CI 0.377–0.967, P = 0.036), self-care ability (OR = 5.881, 95% CI 1.867–18.529, P = 0.002), and annual physical examinations (OR = 3.167, 95% CI 2.31–4.34, P < 0.001) were independently associated with willingness to self-manage EHRs. Annual physical examination (OR = 2.507, 95%CI 1.585–2.669, P < 0.001) also independently made a difference to the awareness of EHRs.

Conclusions

Our findings suggest that annual physical examinations, age, hearing problems, and self-care abilities are significant factors in assessing individuals’ awareness and adoption of EHRs. Understanding the characteristics of individuals who are aware of or are willing to take advantage of EHRs plays a positive role in promoting their popularization and application.
Job boredom as an antecedent of four states of mental health: life satisfaction, positive functioning, anxiety, and depression symptoms among young employees – a latent change score approach
Authors: Li Jie , Kaltiainen Janne , Hakanen Jari J.
Published: March 2024

Background

Job boredom has been generally associated with poorer self-rated health but the evidence is mainly cross-sectional and there is a lack of a holistic mental health approach. We examined the temporal relationships between job boredom and mental health indicators of life satisfaction, positive functioning, anxiety, and depression symptoms.

Methods

We analyzed a two-wave postal survey data of adults aged 23 to 34 that was collected from the Finnish working population between 2021 and 2022 (n = 513). Latent change score modelling was used to estimate the effects of prior levels of job boredom on subsequent changes in mental health indicators, and of prior levels of mental health indicators on subsequent changes in job boredom.

Results

Job boredom was associated with subsequent decreases in life satisfaction and positive functioning and increases in anxiety and depression symptoms. Of these associations, job boredom was more strongly associated with changes in positive functioning and anxiety symptoms than with changes in life satisfaction.

Conclusions

Our two-wave study suggests that job boredom, a motivational state of ill-being in the work domain, spills over into general mental health by decreasing life satisfaction and positive functioning and increasing anxiety and depression symptoms. Our findings contribute to the understanding of the potential detrimental effects of job boredom and its nomological network. From a practical perspective, workplaces are adviced to improve working conditions that mitigate job boredom and thus promote employees’ mental health.
Lumbosacral transitional vertebra in spondylolisthesis: frequency, demographic findings, and clinical characteristics
Authors: Mahmoodkhani Mehdi , Naeimi Arvin , Zohrevand Amirhossein , Rabbanifard Arian , Rezvani Majid
Published: March 2024

Background

The association of LSTV with low back pain has been debated in the literature for nearly a century, but the relationship between LSTV and spondylolisthesis is still under discussion. There is currently no valid information about LSTV’s prevalence in Iran. This study investigated the relationship between the presence of LSTV and lumbosacral spondylolisthesis regarding frequency, gender and age variation, grade and level of spondylolisthesis, and clinical signs and symptoms.

Methods

This cross-sectional study included spondylolisthesis patients admitted for surgery between March 2021 to December 2022. All patients underwent CT imaging. After evaluating medical records, the baseline data were collected. Patients were categorized into No LSTV, Sacralization, and Lumbarization groups. Demographic and clinical characteristics of the studied groups were compared using an independent T-test and Chi-Square. Multiple logistic regression was used to assess the age and sex variations between groups.

Results

219 patients with a mean age of 57.07 ± 11.04 were included. A significant relationship was observed between the presence of sacralization and gender diversity with female predominance (P = 0.01). The level of spondylolisthesis and the presence of motor deficits (paresis) significantly differed among study groups (P < 0.05). Sacralization group exhibited a greater prevalence of higher grades of listhesis compared to the other groups.

Conclusions

LSTV is frequently seen in spondylolisthesis patients. Sacralization is the common type of LSTV in spondylolisthesis patients, possibly leading to an increased risk for higher grades of vertebral slip and higher rates of motor deficit signs and symptoms. The presence of sacralization results in a significant increase in the incidence of higher levels of spondylolisthesis, especially the L4-L5*(sacralized L5) level. There is no relationship between age and the presence of LSTV in spondylolisthesis.
Ultrasound-guided percutaneous transhepatic one-step biliary fistulation combined with rigid choledochoscopy for recurrent hepatolithiasis
Authors: Pan Wu , Lu Yuhang , Li JunJie , Zhang Jia , Chen Shenglin
Published: March 2024

Purpose

Percutaneous transhepatic one-step biliary fistulation (PTOBF) is used to treat choledocholithiasis and biliary stricture. This study aimed to evaluate the safety and efficacy of ultrasound-guided PTOBF combined with rigid choledochoscopy in the treatment of recurrent hepatolithiasis.

Materials and methods

The clinical data of 37 consecutive patients who underwent PTOBF combined with rigid choledochoscopy for RHL from March 2020 to March 2022 at our hospital were retrospectively analyzed.

Results

A total of 68 percutaneous transhepatic punctures were performed in 37 patients, with a puncture success rate of 85.29% (58/68) and a dilatation success rate of 100.00% (58/58). The mean blood loss of operation was 9.84 ± 18.10 mL, the mean operation time was 82.05 ± 31.92 min, and the mean length of postoperative hospital stay was 5.59 ± 3.26 days. The initial stone clearance rate was 40.54% (15/37) and the final stone clearance rate was 100% (37/37). The incidence of postoperative complications was 10.81% (4/37), including 2 cases of pleural effusion, 1 case of hemorrhage, and 1 case of cholangitis, which recovered after treatment. During a mean follow-up period of 23 months (range 12 to 36 months), only 1 patient experienced stone recurrence.

Conclusion

Ultrasound-guided PTOBF combined with rigid choledochoscopy in the treatment of RHL based on skilful manipulation seems to be a safe, effective and minimally invasive method with clinical application value. Further comparative studies with large sample sizes are needed in the future to confirm the reliability of its therapeutic results.
Symptoms and quality of life among men starting treatment for metastatic castration-resistant prostate cancer – a prospective multicenter study
Authors: Rönningås Ulrika , Holm Maja , Fransson Per , Beckman Lars , Wennman-Larsen Agneta
Published: March 2024

Background

Men with metastatic castration-resistant prostate cancer (mCRPC) have an incurable disease. Along with prolonging life, symptom management is one of the main goals with treatment. This is also important from a palliative care perspective where the life prolonging outcomes should be balanced with quality of life (QoL) in this late phase. It is also essential in symptom management to view different dimensions of symptoms, for example how severe or distressing symptoms are, to support best QoL. Therefore, more knowledge is needed about the symptom experience when these treatments are initiated and thus the aim of this study was to describe different dimensions of symptoms in men with mCRPC starting their first-line of life-prolonging treatment, and to describe the association between symptom burden and QoL.

Methods

Baseline data from a prospective longitudinal study of 143 men with mCRPC starting their first-line life-prolonging treatment were used. Symptoms were measured using the Memorial Symptom Assessment Scale (MSAS) and global QoL was measured by the EORTC QLQ C-30. Data was analyzed using descriptive- and multivariable linear regression analyses.

Results

On average, the men had more than 10 symptoms (range 0–31 of 33). 50% or more reported sweats, lack of energy, pain, problems with sexual activity and sexual desire. The symptoms they reported as most severe, or most distressing were not always the ones that were reported as most frequent. There was an association between QoL and physical symptoms, and also between QoL, and analgesic use and prostate-specific antigen (PSA) values.

Conclusion

Even if some men with mCRPC report many symptoms, the dimensions of severity and distress levels vary, and the most frequent symptoms was not always the most burdensome or distressing. There was an association between high physical symptom burden and QoL, suggesting that it is not the number of symptoms that affects QoL but rather the subjective perceived impact of the physical symptoms experienced. The knowledge of how men with mCRPC experience and perceive their symptoms may help health care professionals in symptom management aiming to improve QoL, which is a cornerstone in integrating early palliative care.
“Access to pharmacy services is difficult in China”: a qualitative study from the perspective of transplant recipients to explore their expectations
Authors: Yin Zhao , Zhang Wan , Jia Xuedong , Yang Xi , Guo Wenzhi , Fu Hang , Du Shuzhang , Zhang Xiaojian , Si Fangying , Li Jun
Published: March 2024

Purpose

To gain an in-depth and comprehensive understanding of Chinese organ transplant recipients’ perceptions, expectations, and suggestions of pharmacy services to hospital pharmacists.

Methods

This qualitative study was conducted in central China, from February to December 2020. Participants were collected with a purposive and snowball sampling method. Focus group discussions were conducted with organ transplant recipients and content analysis was applied to identify themes and subthemes.

Results

21 recipients participated in the qualitative study. Four themes and thirteen subthemes were identified: (1) perceptions of clinical pharmacists and pharmacy services; (2) expectations for pharmacy service content; (3) expectations for pharmacy service form; and (4) difficulties as a special group.

Conclusion

The pharmacy services provided by Chinese healthcare institutions are inadequate to meet the needs of organ transplant recipients. However, the acceptance and expectation of pharmacy services by transplant recipients are high. Therefore, China should learn from the experience of developed countries and focus on the actual needs of patients to establish a better pharmacy service system for organ transplantation.
Development of consensus quality indicators for cancer supportive care: a Delphi study and pilot testing
Authors: Hyatt Amelia , Gough Karla , Chung Holly , Wood Wendy , Aston Ruth , Cockwill Jo , Galetakis Spiridoula , Krishnasamy Meinir
Published: March 2024

Background

High quality supportive care is fundamental to achieve optimal health outcomes for people affected by cancer. Use of quality indicators provides comparative information for monitoring, management, and improvement of care within and across healthcare systems. The aim of this Australian study was to develop and test a minimum viable set of cancer supportive care quality indicators that would be feasible to implement and generate usable data for policy and practice.

Methods

A two-round, modified reactive Delphi process was employed firstto develop the proposed indicators. Participants with expertise in cancer control in Australia, the United Kingdom, and Canada rated their level of agreement on a 7-point Likert scale against criteria assessing the importance, feasibility, and usability of proposed indicators. Relative response frequencies were assessed against pre-specified consensus criteria and a ranking exercise, which delivered the list of proposed indicators. Draft indicators were then presented to a purposive sample of clinicial and health management staff via qualitative interviews at two acute care settings in Melbourne, Australia for feedback regarding feasibility. Desktop audits of online published health service policy and practice descriptions were also conducted at participating acute care settings to confirm health service data availability and feasibility of collection to report against proposed indicators.

Results

Sixteen quality indicators associated with the delivery of quality cancer supportive care in Australian acute healthcare settings met pre-specified criteria for inclusion. Indicators deemed ‘necessary’ were mapped and ranked across five key categories: Screening, Referrals, Data Management, Communication and Training, and Culturally Safe and Accessible Care. Testing confirmed indicators were viewed as feasible by clinical and health management staff, and desktop audits could provide a fast and reasonably effective method to assess general adherence and performance.

Conclusions

The development of quality indicators specific to cancer supportive care provides a strong framework for measurement and monitoring, service improvement, and practice change with the potential to improve health outcomes for people affected by cancer. Evaluation of implementation feasibility of these expert consensus generated quality indicators is recommended.
From gene to mechanics: a comprehensive insight into the mechanobiology of LMNA mutations in cardiomyopathy
Authors: Veltrop R. J. A. , Kukk M. M. , Topouzidou K. , Didden L. , Muchir A. , van Steenbeek F. G. , Schurgers L. J. , Harakalova M.
Published: March 2024

Severe cardiac remodeling leading to heart failure in individuals harboring pathogenic LMNA variants, known as cardiolaminopathy, poses a significant clinical challenge. Currently, there is no effective treatment for lamin-related diseases. Exploring the intricate molecular landscape underlying this condition, with a specific focus on abnormal mechanotransduction, will propel our understanding of cardiolaminopathy. The LMNA gene undergoes alternative splicing to create A-type lamins, a part of the intermediate filament protein family. A-type lamins are located underneath the nuclear envelope, and given their direct interaction with chromatin, they serve as mechanosensory of the cell by interacting with the cytoskeleton and safeguarding the transcriptional program of cells. Nucleated cells in the cardiovascular system depend on precise mechanical cues for proper function and adaptation to stress. Mechanosensitive signaling pathways are essential in regulating mechanotransduction. They play a pivotal role in various molecular and cellular processes and commence numerous downstream effects, leading to transcriptional activation of target genes involved in proliferation, migration, and (anti-)apoptosis. Most pathways are known to be regulated by kinases, and this area remains largely understudied in cardiomyopathies.

Heart failure is linked to disrupted mechanotransduction, where LMNA mutations affect nuclear integrity, impacting the response to extracellular matrix signals and the environment. The Hippo pathway, anchored by YAP1/WWTR1, emerges as a central player by orchestrating cellular responses to mechanical signals. However, the involvement of Hippo and YAP1/WWTR1 in cardiolaminopathy is unclear and likely mutation- and tissue-specific, warranting further investigation. Here, we highlight the involvement of multiple signaling pathways in mechanotransduction in cardiolaminopathy. We delve into (non-)canonical functions of key signaling components, which may hold critical clues for understanding disease pathogenesis. In summary, we comprehensively examine the mechanobiology of A-type lamins, the role of mechanosensitive signaling pathways, and their intricate interplay in the pathogenesis of cardiolaminopathy. A better understanding of these mechanisms is paramount for developing targeted therapies and interventions for individuals afflicted with this debilitating cardiac condition. Prior studies overlooked accurate gene nomenclature in protein and pathway names. Our review addresses this gap, ensuring precision by aligning names with correct gene nomenclature.
Frizzled class receptor 5 contributes to ovarian cancer chemoresistance through aldehyde dehydrogenase 1A1
Authors: Xia Yuhong , Wang Shan , Sun Yu , Wang Wei , Chang Shijie , Zhang Zhongbo , Zhao Chenghai
Published: March 2024

Background

Chemoresistance is associated with tumor relapse and unfavorable prognosis. Multiple mechanisms underlying chemoresistance have been elucidated, including stemness and DNA damage repair. Here, the involvement of the WNT receptor, FZD5, in ovarian cancer (OC) chemoresistance was investigated.

Methods

OC cells were analyzed using in vitro techniques including cell transfection, western blot, immunofluorescence and phalloidin staining, CCK8 assay, colony formation, flowcytometry, real-time PCR, and tumorisphere formation. Pearson correlation analysis of the expression levels of relevant genes was conducted using data from the CCLE database. Further, the behavior of OC cells in vivo was assessed by generation of a mouse xenograft model.

Results

Functional studies in OC cells showed that FZD5 contributes to epithelial phenotype maintenance, growth, stemness, HR repair, and chemoresistance. Mechanistically, FZD5 modulates the expression of ALDH1A1, a functional marker for cancer stem-like cells, in a β-catenin-dependent manner. ALDH1A1 activates Akt signaling, further upregulating RAD51 and BRCA1, to promote HR repair.

Conclusions

Taken together, these findings demonstrate that the FZD5-ALDH1A1-Akt pathway is responsible for OC cell survival, and targeting this pathway can sensitize OC cells to DNA damage-based therapy.
Deep learning model for classifying shoulder pain rehabilitation exercises using IMU sensor
Authors: Lee Kyuwon , Kim Jeong-Hyun , Hong Hyeon , Jeong Yeji , Ryu Hokyoung , Kim Hyundo , Lee Shi-Uk
Published: March 2024

Background

Artificial intelligence is being used for rehabilitation, including monitoring exercise compliance through sensor technology. AI classification of shoulder exercise wearing an IMU sensor has only been reported in normal (i.e. painless) subjects. To prove the feasibility of monitoring exercise compliance, we aimed to classify 11 types of shoulder rehabilitation exercises using an AI (artificial intelligence) algorithm in patients with shoulder pain. We had the patients wear an IMU-based sensor, collected data during exercise, and determined the accuracy of exercise classification.

Methods

Data were collected from 58 patients (27 males, 31 females, age range 37–82 years) diagnosed with shoulder diseases such as adhesive capsulitis and rotator cuff disease. 11 types of shoulder pain rehabilitation exercise programs were developed and repeated each exercise ten times per session while wearing an IMU sensor. The study applied the Rectified Linear Unit (ReLU) and the SoftMax as the activation function for hidden layers, the output layer.

Results

The acquired data was used to train a DNN model using the multilayer perceptron algorithm. The trained model was used to classify 11 types of shoulder pain rehabilitation exercises. The training accuracy was 0.975 and the test accuracy was 0.925.

Conclusion

The study demonstrates that IMU sensor data can effectively classify shoulder pain rehabilitation exercises, providing more appropriate feedback for patients. The model can be utilized to establish a system for remotely monitoring patients’ exercise performance. The use of deep learning in patient monitoring and rehabilitation has significant potential to bring innovative changes to healthcare service delivery.
CT comparison of the nasal airway anterior and posterior to the piriform aperture in patients with and without nasal obstruction
Authors: Heppt Helen , Widmann Gerlig , Riechelmann Felix , Runge Annette , Riechelmann Herbert , Giotakis Aris I.
Published: March 2024

Background

Nasal airway stenosis may lie anterior and/or posterior to the piriform aperture. We intended to compare the nasal airway anterior and posterior to the piriform aperture in patients with and without nasal obstruction.

Methods

Segmented computed tomography cross-sectional areas of the nasal airway anterior (CT-CSAant) and posterior to the piriform aperture (at the level of the head of the inferior turbinate; CT-CSApost) were compared between patients with nasal obstruction (cases) and trauma controls. CT-CSA were approximately perpendicular to the direction of the nasal airflow. Anterior to the piriform aperture, they were tilted about 30o, 60o and 90o to the nasal floor. Posterior to the piriform aperture, they were tilted about 50o, 80o and 100o to the nasal floor. In cases, we examined the Pearson’s correlation of active anterior rhinomanometry with CT-CSAant and CT-CSApost.

Results

Narrow and bilateral CT-CSApost were similarly large between 56 cases and 56 controls (all p > 0.2). On the contrary, narrow and bilateral CT-CSAant were significantly smaller in cases than in controls (all p < 0.001). The ratio of the size of CT-CSAant−30 to that of CT-CSApost−80 was significantly lower in cases (median: 0.84; lower to upper quartile: 0.55–1.13) than in controls (1.0; 0.88–1.16; Mann-Whitney U test; p = 0.006). Bilateral CT-CSAant correlated significantly with total inspiratory flow (all p < 0.026) in contrast to bilateral CT-CSApost (all p > 0.056).

Conclusions

The nasal airway anterior to the piriform aperture was smaller in patients with nasal obstruction due to skeletal nasal stenosis than that in controls. On the contrary, the nasal airway posterior to the piriform aperture was similarly large between patients with and without nasal obstruction. Furthermore, in patients with nasal obstruction, the anterior nasal airway was narrower compared to that located posterior to it. On the contrary, control patients’ anterior nasal airway was as large as the posterior one.