bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical characteristics and outcome of dural arteriovenous fistulas secondary to cerebral venous sinus thrombosis: a primary or secondary event?
Authors: Huang Xiaoqin , Shen Huixin , Fan Chunnqiu , Chen Jian , Meng Ran
Published: March 2023

Background

The Dural Arteriovenous Fistulas (DAVFs) secondary to cerebral venous sinus thrombosis (CVST) are rather rare. The aim of present study is to investigate the clinical and radiological features, and treatment outcome of DAVFS in patients following CVST.

Methods

Data about demographic information, clinical presentations, radiological findings, as well as treatment and outcome of DAVFs sequence to CVST were collected to analysis from January 2013 to September 2020 in this retrospective study.

Results

Fifteen patients with DAVFs after CVST were included in the study. The median age was 41 years (range17-76 years). Ten patients (66.67%) were male and 6 patients (33.33%) were female. The median duration of presenting CVST was 182 days (Range 20–365). Mean time from diagnosis of CVST to confirmation of DAVFs was 97 days (range 36–370 days). The most common manifestations of DAVFs following CVST were headache and visual disturbance seen in 7 patients respectively. Five patients had pulsatile tinnitus (%) and 2 had nausea/vomiting. The DAVFs are most frequently located at the transverse/sigmoid sinus (7/15, 46.67%), followed by the superior sagittal the sinus and confluence sinus (6/15, 40.00%) respectively. Angiography of DAVFs revealed Board type I in seven (46.7%) patients, Board type II and III in 4(26.7%) patients, respectively. The Cognard I was noted in seven (46.7%), Cognard IIa and IV in 3 patients, IIb and III in one patient, respectively. The main feeding arteries of DAVFs most commonly originate from the branches of the external carotid artery in 6 (40.0%) patients. The other DAVFs are conjointly supplied by multiple feeders from internal and external carotid artery and vertebral arteries. Fourteen (93.33%) patients were treated with endovascular embolization and none of the patients had permanent deficits during follow-up.

Conclusion

Intracranial DAVFs following CVST are rare presentations. Most patients have a good outcome after timely interventional therapy. Continued observation and follow-up of (DSA) are important to find DAVFs secondary to CVST.
Rural-urban differentials of prevalence and lifestyle determinants of pre-diabetes and diabetes among the elderly in southwest China
Authors: Zhao Yi , Li Hui-fang , Wu Xia , Li Guo-hui , Golden Allison Rabkin , Cai Le
Published: March 2023

Background

Diabetes has become a major public health problem in China. A better understanding of diabetes determinants and urban-rural differences is essential to crafting targeted diabetes prevention measures for the elderly living in both urban and rural areas. This study aimed to compare rural-urban differentials in prevalence and lifestyle determinants of pre-diabetes and diabetes among the elderly in southwest China.

Methods

A cross-sectional health interview and examination survey was conducted among individuals aged ≥ 60 years in both a rural and urban area of China. Anthropometric measurements, including height, weight, and waist circumference, as well as blood pressure and fasting blood glucose measurements were taken. Associated risk factors for pre-diabetes and diabetes were evaluated using multivariate logistic regression analysis.

Results

In total, 1,624 urban residents and 1,601 rural residents consented to participate in the study. The urban prevalence of pre-diabetes and diabetes (46.8% and 24.7%, respectively), was higher than the rural prevalence (23.4% and 11.0%, respectively, P<0.01). Urban elderly participants had markedly higher prevalence of obesity, central obesity, and physical inactivity than their rural counterparts (15.3%, 76.0%, and 9.2% vs. 4.6%, 45.6%, and 6.1%, P<0.01). In contrast, rural elderly adults had higher prevalence of smoking than urban ones (23.2% vs. 17.2%, P<0.01). Obese (OR 1.71, 95% CI 1.27–2.30 vs. OR 1.73, 95% CI 1.30–3.28) and centrally obese participants (OR 1.59, 95% CI 1.18–2.15 vs. OR 1.83, 95% CI 1.32–2.54) were more likely to suffer from diabetes in both urban and rural regions. Furthermore, urban current smokers had a higher probability of suffering from diabetes (OR 1.58, 95% CI 1.11–2.25), while hypertension was positively associated with the prevalence of diabetes in the rural area (OR 2.13, 95% CI 1.54–2.95). Obese participants in the rural area were more likely to suffer from pre-diabetes (OR 2.50, 95% CI 1.53–4.08), while physical inactivity was positively associated with prevalence of pre-diabetes in the urban area (OR 1.95, 95% CI 1.37–2.80).

Conclusion

Pre-diabetes and diabetes are more prevalent among urban older adults than their rural counterparts in southwest China. The identified rural-urban differentials of lifestyle factors have significant impacts on prevalence of pre-diabetes and diabetes. Thus, tailored lifestyle interventions are needed to improve diabetes prevention and management among the elderly in southwest China.
Peri-implant diseases diagnosis, prognosis and dental implant monitoring: a narrative review of novel strategies and clinical impact
Authors: Bornes Rita , Montero Javier , Correia André , Marques Tiago , Rosa Nuno
Published: March 2023

Background

The diagnosis of peri-implantar and periodontal relies mainly on a set of clinical measures and the evaluation of radiographic images. However, these clinical settings alone are not sufficient to determine, much less predict, periimplant bone loss or future implant failure. Early diagnosis of periimplant diseases and its rate of progress may be possible through biomarkers assessment. Once identified, biomarkers of peri-implant and periodontal tissue destruction may alert the clinicians before clinical signs show up. Therefore, it is important to consider developing chair-side diagnostic tests with specificity for a particular biomarker, indicating the current activity of the disease.

Methods

A search strategy was created at Pubmed and Web of Science to answer the question: “How the molecular point-of-care tests currently available can help in the early detection of peri-implant diseases and throws light on improvements in point of care diagnostics devices?”

Results

The PerioSafe® PRO DRS (dentognostics GmbH, Jena) and ImplantSafe® DR (dentognostics GmbH, Jena ORALyzer® test kits, already used clinically, can be a helpful adjunct tool in enhancing the diagnosis and prognosis of periodontal/peri-implantar diseases. With the advances of sensor technology, the biosensors can perform daily monitoring of dental implants or periodontal diseases, making contributions to personal healthcare and improve the current status quo of health management and human health.

Conclusions

Based on the findings, more emphasis is given to the role of biomarkers in diagnosing and monitoring periodontal and peri-implant diseases. By combining these strategies with traditional protocols, professionals could increase the accuracy of early detection of peri-implant and periodontal diseases, predicting disease progression, and monitoring of treatment outcomes.
Palliative care in severe mental illnesses
Authors: Masel Eva Katharina , Antunes Bárbara , Schulz-Quach Christian
Published: March 2023

In this editorial, we highlight the interaction between patients who are diagnosed with severe mental illness and their treatment within palliative care, a clinical area of specialized focus which has a multitude of complex impacts on affected patients, their (chosen) family members and caregivers, as well as the healthcare professionals who are caring for them.
The mediating effects of attitude toward death and meaning of life on the relationship between perception of death and coping with death competence among Chinese nurses: a cross-sectional study
Authors: He Shijia , Zhao Hong , Wang Huiping , Chen Fengzhi , Lv Tingting , Li Lezhi , Zhang Huilin
Published: March 2023

Background

It is important to understand how the perception of death affects the competence to cope with death.

Objectives

To explore whether the perception of death has an indirect effect on competence to cope with death through the mediation of attitude toward death and meaning of life.

Methods

A total of 786 nurses from Hunan Province, China, selected by random sampling method and asked to complete an online electronic questionnaire between October and November 2021 were included in the study.

Results

The nurses’ scored 125.39 ± 23.88 on the competence to cope with death. There was a positive correlation among perception of death, competence to cope with death, the meaning of life, and attitude toward death. There were three mediating pathways: the separate mediating effect of natural acceptance and meaning of life, and the chain mediating effect of natural acceptance and meaning of life.

Conclusion

The nurses’ competence to cope with death was moderate. Perception of death could indirectly and positively predict nurses’ competence to cope with death by enhancing natural acceptance or sense of meaning in life. In addition, perception of death could improve natural acceptance and then enhance the sense of meaning in life to positively predict nurses’ competence to cope with death.
Pre-referral rectal artesunate is no “magic bullet” in weak health systems
Authors: Hetzel Manuel W. , Okitawutshu Jean , Tshefu Antoinette , Omoluabi Elizabeth , Awor Phyllis , Signorell Aita , Kwiatkowski Marek , Lambiris Mark J. , Visser Theodoor , Cohen Justin M. , Buj Valentina , Burri Christian , Lengeler Christian
Published: March 2023

Severe malaria is a potentially fatal condition that requires urgent treatment. In a clinical trial, a sub-group of children treated with rectal artesunate (RAS) before being referred to a health facility had an increased chance of survival. We recently published in BMC Medicine results of the CARAMAL Project that did not find the same protective effect of pre-referral RAS implemented at scale under real-world conditions in three African countries. Instead, CARAMAL identified serious health system shortfalls that impacted the entire continuum of care, constraining the effectiveness of RAS. Correspondence to the article criticized the observational study design and the alleged interpretation and consequences of our findings.

Here, we clarify that we do not dispute the life-saving potential of RAS, and discuss the methodological criticism. We acknowledge the potential for confounding in observational studies. Nevertheless, the totality of CARAMAL evidence is in full support of our conclusion that the conditions under which RAS can be beneficial were not met in our settings, as children often failed to complete referral and post-referral treatment was inadequate.

The criticism did not appear to acknowledge the realities of highly malarious settings documented in detail in the CARAMAL project. Suggesting that trial-demonstrated efficacy is sufficient to warrant large-scale deployment of pre-referral RAS ignores the paramount importance of functioning health systems for its delivery, for completing post-referral treatment, and for achieving complete cure. Presenting RAS as a “magic bullet” distracts from the most urgent priority: fixing health systems so they can provide a functioning continuum of care and save the lives of sick children.

The data underlying our publication is freely accessible on Zenodo.
Up-regulated CD38 by daphnetin alleviates lipopolysaccharide-induced lung injury via inhibiting MAPK/NF-κB/NLRP3 pathway
Authors: Guo Yujie , Zhang Huiqing , Lv Zhe , Du Yuna , Li Dan , Fang Hui , You Jing , Yu Lijun , Li Rong
Published: March 2023

Background

Sepsis is a life-threatening organ dysfunction syndrome resulted from severe infection with high morbidity and mortality. Cluster of differentiation 38 (CD38) is a multifunctional type II transmembrane glycoprotein widely expressed on the surface of various immunocytes membranes that mediates host immune response to infection and plays an important role in many inflammatory diseases. Daphnetin (Daph), isolated from the daphne genus plant, is a natural coumarin derivative that possesses anti-inflammatory and anti-apoptotic effects. The current study aimed to investigate the role and mechanism of Daph in alleviating lipopolysaccharide (LPS)-induced septic lung injury, and to explore whether the protective effect of Daph in mice and cell models was related to CD38.

Methods

Firstly, network pharmacology analysis of Daph was performed. Secondly, LPS-induced septic lung injury in mice were treated with Daph or vehicle control respectively and then assessed for survival, pulmonary inflammation and pathological changes. Lastly, Mouse lung epithelial cells (MLE-12 cells) were transfected with CD38 shRNA plasmid or CD38 overexpressed plasmid, followed by LPS and Daph treatment. Cells were assessed for viability and transfection efficiency, inflammatory and signaling.

Results

Our results indicated that Daph treatment improved survival rate and alleviated pulmonary pathological damage of the sepsis mice, as well as reduced the excessive release of pro-inflammatory cytokines IL-1β, IL-18, IL-6, iNOS and chemokines MCP-1 regulated by MAPK/NF-κB pathway in pulmonary injury. Daph treatment decreased Caspase-3 and Bax, increased Bcl-2, inhibited nucleotide-binding domain (NOD)-like receptor protein 3 (NLRP3) inflammasome‐mediated pyroptosis in lung tissues of septic lung injury. Also, Daph treatment reduced the level of excessive inflammatory mediators, inhibited apoptosis and pyroptosis in MLE-12 cells. It is noteworthy that the protective effect of Daph on MLE-12 cells damage and death was assisted by the enhanced expression of CD38.

Conclusions

Our results demonstrated that Daph offered a beneficial therapeutic effect for septic lung injury via the up-regulation of CD38 and inhibition of MAPK/NF-κB/NLRP3 pathway.
Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders
Authors: Lun Jingxian , Li Yubin , Gao Xuefeng , Gong Zelong , Chen Xiaoliang , Zou Jinhu , Zhou Chengxing , Huang Yuanyuan , Zhou Bingliang , Huang Pengwei , Cao Hong
Published: March 2023

Despite extensive astrocyte activation in patients suffering from HIV-associated neurocognitive disorders (HAND), little is known about the contribution of astrocytes to HAND neuropathology. Here, we report that the robust activation of neurotoxic astrocytes (A1 astrocytes) in the CNS promotes neuron damage and cognitive deficits in HIV-1 gp120 transgenic mice. Notably, knockout of α7 nicotinic acetylcholine receptors (α7nAChR) blunted A1 astrocyte responses, ultimately facilitating neuronal and cognitive improvement in the gp120tg mice. Furthermore, we provide evidence that Kynurenic acid (KYNA), a tryptophan metabolite with α7nAChR inhibitory properties, attenuates gp120-induced A1 astrocyte formation through the blockade of α7nAChR/JAK2/STAT3 signaling activation. Meanwhile, compared with gp120tg mice, mice fed with tryptophan showed dramatic improvement in cognitive performance, which was related to the inhibition of A1 astrocyte responses. These initial and determinant findings mark a turning point in our understanding of the role of α7nAChR in gp120-mediated A1 astrocyte activation, opening up new opportunities to control neurotoxic astrocyte generation through KYNA and tryptophan administration.
Evaluation of ten (10) SARS-CoV-2 rapid serological tests in comparison with WANTAI SARS-CoV-2 ab ELISA in Burkina Faso, West Africa
Authors: Ouedraogo Henri Gautier , Zoure Abdou Azaque , Compaoré Tegwinde Rebeca , Ky Herve , Zida Sylvie , Zingué Dezemon , Ouedraogo Oumarou , Soubeiga Serge Théophile , Sagna Tani , Dabiré Charlemagne , Kambiré Dinanibè , Zongo Dramane , Yonli Albert Théophane , Nikiema Abdoul Rahamani , Nezien Désiré , Bansé Gnintassa Cyrille , Bicaba Brice Wilfried , Perier Sophie , Sawadogo Charles , Yabre Zakariya , Sangare Lassana
Published: March 2023

Background

The aim of this study was to evaluate the performance of ten (10) SARS-CoV-2 serological rapid diagnostic tests in comparison with the WANTAI SARS-CoV-2 Ab ELISA test in a laboratory setting.

Materials and methods

Ten (10) SARS-CoV-2 serological rapid diagnostic tests (RDTs) for SARS-CoV-2 IgG/IgM were evaluated with two (2) groups of plasma tested positive for one and negative for the other with the WANTAI SARS-CoV-2 Ab ELISA. The diagnostic performance of the SARS-CoV-2 serological RDTs and their agreement with the reference test were calculated with their 95% confidence intervals.

Results

The sensitivity of serological RDTs ranged from 27.39 to 61.67% and the specificity from 93.33 to 100% compared to WANTAI SARS-CoV-2 Ab ELISA test. Of all the tests, two tests (STANDARD Q COVID-19 IgM/IgG Combo SD BIOSENSOR and COVID-19 IgG/IgM Rapid Test (Zhejiang Orient Gene Biotech Co., Ltd)) had a sensitivity greater than 50%. In addition, all ten tests had specificity greater than or equal to 93.33% each. The concordance between RDTs and WANTAI SARS-CoV-2 Ab ELISA test ranged from 0.25 to 0.61.

Conclusion

The SARS-CoV-2 serological RDTs evaluated show low and variable sensitivities compared to the WANTAI SARS-CoV-2 Ab ELISA test, with however a good specificity. These finding may have implications for the interpretation and comparison of COVID-19 seroprevalence studies depending on the type of test used.
Vertebral bone quality score to predict cage subsidence following oblique lumbar interbody fusion
Authors: Huang Yong , Chen Qian , Liu Limin , Feng Ganjun
Published: March 2023

Background

Current evidence suggests that the magnetic resonance imaging (MRI)-based vertebral bone quality (VBQ) score is a good parameter for evaluating bone quality. We aimed to assess whether the VBQ score can predict the occurrence of postoperative cage subsidence after oblique lumbar interbody fusion (OLIF) surgery.

Methods

Patients (n = 102) who had undergone single-level OLIF with a minimal follow-up for 1 year were reviewed in this study. Demographic and radiographic data of these patients were collected. Cage subsidence was defined as ≥ 2 mm of cage migration into the inferior endplate, superior endplate, or both. Further, the MRI-based VBQ score was measured on T1-weighted images. Moreover, univariable and multivariable binary logistic regression analyses were performed. Meanwhile, Pearson analysis was used to evaluate the correlation among the VBQ score, average lumbar dual-energy X-ray absorptiometry (DEXA) T-score, and degree of cage subsidence. Furthermore, ad-hoc analysis was used along with receiver operating characteristic curve analysis to assess the predictive ability of the VBQ score and average lumbar DEXA T-score.

Results

Of 102 participants, cage subsidence was observed in 39 (38.24%) patients. According to the univariable analysis, patients with subsidence had older age, higher antiosteoporotic drug use, larger disk height change, a more concave morphology of inferior and superior endplates, higher VBQ score, and lower average lumbar DEXA T-score compared to patients without subsidence. In the multivariable logistic regression analysis, a higher VBQ score was significantly associated with an increased risk of subsidence (OR = 23.158 ± 0.849, 95% CI 4.381–122.399, p < 0.001), and it was the only significant and independent predictor of subsidence after OLIF. Moreover, the VBQ score was moderately correlated with the average lumbar DEXA T-score (r = − 0.576, p < 0.001) and the amount of cage subsidence (r = 0.649, p < 0.001). Furthermore, this score significantly predicted cage subsidence with an accuracy of 83.9%.

Conclusions

The VBQ score can independently predict postoperative cage subsidence in patients undergoing OLIF surgery.
EPB41L4A-AS1 and UNC5B-AS1 have diagnostic and prognostic significance in osteosarcoma
Authors: Yan Ying , Liu Xiaochuan , Li Yamei , Yan Jingyi , Zhao Ping , Yang Lu
Published: March 2023

Background

Deregulation of lncRNAs has been observed in human osteosarcoma. This study explored the diagnostic and prognostic significance of EPB41L4A-AS1 and UNC5B-AS1 in osteosarcoma.

Methods

Relative levels of EPB41L4A-AS1 and UNC5B-AS1 were detected in osteosarcoma tissue samples and cells. The ability to distinguish osteosarcoma from health was assessed by receiver operating characteristic (ROC) curve construction. Kaplan–Meier (K–M) and Cox proportional-hazards analyses were performed for prognosis factors. The bioinformatics approach was used to identify targeting miRNA for EPB41L4A-AS1 and UNC5B-AS1. Kaplan–Meier survival curves and Whitney Mann U tests were conducted for validating the statistical significance. In cell culture experiments, the influence of EPB41L4A-AS1 and UNC5B-AS1 on proliferation, migration, and invasion of the osteosarcoma cell line was examined by CCK-8 and Transwell assays.

Results

Levels of EPB41L4A-AS1 and UNC5B-AS1 were upregulated in osteosarcoma patients and cells compared with the healthy participants and normal cell lines. EPB41L4A-AS1 and UNC5B-AS1 have a potent ability to distinguish the patients with osteosarcoma from the health. EPB41L4A-AS1 and UNC5B-AS1 levels correlated with SSS stage. Patients with high levels of EPB41L4A-AS1 and UNC5B-AS1 had significantly shorter survival times. EPB41L4A-AS1 and UNC5B-AS1 were independent prognostic indexes for overall survival. miR-1306-5p was a common target for EPB41L4A-AS1 and UNC5B-AS1. A propulsive impact on cell proliferation, migration, and invasion by EPB41L4A-AS1 and UNC5B-AS1 was observed, but can be rescued by miR-1306-5p.

Conclusions

It was concluded that upregulations of EPB41L4A-AS1 and UNC5B-AS1 expression were diagnostic and prognostic biomarkers for human osteosarcoma. EPB41L4A-AS1 and UNC5B-AS1 contribute to the biological behavior of osteosarcoma via miR-1306-5p.
Long-term ECMO, efficiency and performance of EUROSETS adult A.L.ONE ECMO oxygenator
Authors: Condello Ignazio , Lorusso Roberto , Nasso Giuseppe , Speziale Giuseppe
Published: March 2023

Background

The management of the oxygenator can be prolonged in the long-term procedures especially during extracorporeal membrane oxygenation (ECMO) for bridge to transplant or bridge to recovery. Long-term use often involves an overrun of the time of use with respect to certification of the oxygenating module of 14 days, for the maintenance of performance and efficiency of the oxygenator. The evaluation of the long-term oxygenator efficiency is complex and depends on the: patient pathology, ECMO configuration, the management of coagulation and anticoagulation, materials selection and circuit components, the structure, design and performance of the oxygenator. In this context we investgated the long-term performance of the A.L.ONE Eurosets ECMO oxygenator in relation to the parameters prodromal to replacement.

Methods

We retrospectively collected eight years data from Anthea Hospital GVM Care & Research, Bari, Italy on the long-term use exceeding 14 days of Eurosets A.L.ONE ECMO Adult oxygenator in Polymetylpentene fiber, for ECMO procedures, including the procedures: Veno Arterial (VA) ECMO post-cardiotomy or not, veno-venous (VV) ECMO. The primary end points were the evaluation of Gas Transfer: oxygen partial pressure (PO2) post oxygenator, Carbon dioxide partial pressure (PCO2) post oxygenator, the oxygen transfer across the oxygenator membrane V′O2, differential CO2 content across oxygenator; Pressure monitoring: oxygenator pressure Drop in relation to Blood flow rate (BFR) (ΔP); Hematologic values: Hemoglobin, Fibrinogen, Platelets, aPTT, D-Dimer, LDH.

Results

Nine VA ECMO patients who used the oxygenator for 18.5 days and two VV ECMO patients who used the oxygenators for 17.2 days on the seventeenth days reported average values PaO2 (267 ± 29 mmHg); PaCO2 (34 ± 4 mmHg) with gas blender values set to 3.8 ± 0.6 L/min of air and a FiO2 of 78 ± 5%; the transfer across the oxygenator membrane V′O2 was 189 ± 43 (ml/min/m2). The mean peak of partial pressure of carbon dioxide from the gas exhaust of oxygenator (PECO2) was 38 ± 4 mmHg; differential CO2 across the oxygenator “pre-oxygenator PCO2–post-oxygenator PCO2” (18 ± 6 mmHg); the mean blood flow rate (BFR) 4.5 ± 0.6 (L/minute); the pump revolution per minutes mean maximum rate was 4254 ± 345 (RPM); the mean pressure drop (ΔP) was 76 ± 12 mmHg; the mean peak of d-dimers (DDs) was 23.6 ± 0.8 mg / dL; the mean peak of LDH was 230 ± 55 (mg/dl); fibrinogen mean peak 223 ± 40 (mg/dl).

Conclusions

The performance of the Eurosets A.L.ONE ECMO Adult polymethylpentene fiber oxygenator in our experience has proven efficiency in terms of O2 uptake and CO2 removal, blood fluid dynamics, metabolic compensation and heat exchange in the long-term treatment. The device was safe without iatrogenic problems over a period of 14 days in the patients undergoing ECMO VA and in all patients undergoing VV ECMO with continuous administration of anticoagulation therapy.
The gender gap in adolescents’ emotional and behavioural problems in Georgia: a cross-sectional study using Achenbach’s Youth Self Report
Authors: Antia Khatia , Račaitė Justina , Šurkienė Genė , Winkler Volker
Published: March 2023

Background

Adolescents, particularly girls, are vulnerable to mental health disorders. Knowledge about young people’s mental health in Eastern European countries is limited. This study is the first to investigate adolescents’ self-reported emotional and behavioural problems in Georgia from a public mental health perspective.

Methods

This study utilized Achenbach’s Youth Self-Reported syndrome scales among 933 adolescents studying from grades 7–12 in 18 public schools in Georgia. We compared the gender-specific results with each other and with the Achenbach’s Normative Sample using two-sample t-tests. Linear regression was used to assess associations between internalizing and externalizing problems and individual as well as demographic characteristics such as parental migration experience (being ‘left-behind’ or ‘staying behind’).

Results

The study found that girls obtained higher scores than boys in Youth Self-Reported empirical syndrome scales and the internalizing broadband scale. Rule-breaking behaviour was the only syndrome scale where boys scored higher. Compared to Achenbach’s Normative Sample, adolescents in Georgia scored higher on all scales. Regression analyses showed that illnesses, having fewer than three close friends, problems at school, and worse relationships with peers/siblings/parents (compared to peers) were associated with higher internalizing and externalizing problems scores in both genders. Performing household chores, living with only one parent, or having a migrant parent showed no associations in either gender.

Conclusion

Emotional and behavioural difficulties of adolescents in Georgia, especially girls, require attention. Having close friends, developing strong relationships with family members, and a supportive school environment could help mitigate emotional and behavioural problems among adolescents in Georgia.
Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology
Authors: Sakaguchi Atsumi , Tanaka Yoichiro , Shoji Eiki , Takeshima Teppei , Sakamaki Rina , Matsuba Takao , Kurihara Yasuyuki
Published: March 2023

Background

Monoclonal antibodies are essential in life science research and developing antibody drugs and test drugs. Various methods have been developed to obtain monoclonal antibodies, among which hybridoma technology continues to be widely used. However, developing a rapid and efficient method for obtaining conformation-specific antibodies using hybridoma technology remains challenging. We previously developed the membrane-type immunoglobulin-directed hybridoma screening (MIHS) method, which is a flow cytometry-based screening technique based on the interaction between the B-cell receptor expressed on the hybridoma cell surface and the antigen protein, to obtain conformation-specific antibodies.

Results

In this study, we proposed a streptavidin-anchored ELISA screening technology (SAST) as a secondary screening method that retains the advantages of the MIHS method. Anti-enhanced green fluorescent protein monoclonal antibodies were generated as a model experiment, and their structural recognition abilities were examined. Examination of the reaction profiles showed that all monoclonal antibodies obtained in this study recognize the conformational epitopes of the protein antigen. Furthermore, these monoclonal antibodies were classified into two groups: those with binding activities against partially denatured proteins and those with complete loss of binding activities. Next, when screening monoclonal antibodies by the MIHS method as the first screening, we found that monoclonal antibodies with stronger binding constants may be selected by double-staining for hybridomas with fluorescently labeled target antigens and fluorescently labeled B cell receptor antibodies.

Conclusions

The proposed two-step screening method, which incorporates MIHS and SAST, constitutes a rapid, simple, and effective strategy to obtain conformation-specific monoclonal antibodies generated through hybridoma technology. The novel monoclonal antibody screening strategy reported herein could accelerate the development of antibody drugs and antibody tests.
Degradation of lignin in different lignocellulosic biomass by steam explosion combined with microbial consortium treatment
Authors: Zhang Wen , Diao Chenyang , Wang Lei
Published: March 2023

The difficulty of degrading lignin is the main factor limiting the high-value conversion process of lignocellulosic biomass. The biodegradation of lignin has attracted much attention because of its strong environmental friendliness, but it still faces some dilemmas such as slow degradation rate and poor adaptability. The microbial consortia with high lignin degradation efficiency and strong environmental adaptability were obtained in our previous research. To further increase the lignin degradation efficiency, this paper proposes a composite treatment technology of steam explosion combined with microbial consortium degradation to treat three kinds of biomass. We measured the lignin degradation efficiency, selectivity value (SV) and enzymatic saccharification efficiency. The structural changes of the biomass materials and microbial consortium structure were also investigated. The experimental results showed that after 1.6 MPa steam explosion treatment, the lignin degradation efficiency of the eucalyptus root reached 35.35% on the 7th days by microbial consortium. At the same time, the lignin degradation efficiency of the bagasse and corn straw treated by steam explosion followed by microbial biotreatment was 37.61–44.24%, respectively, after only 7 days of biotreatment. The microbial consortium also showed strong selectivity degradation to lignin. The composite treatment technology can significantly improve the enzymatic saccharification efficiency. Saccharomycetales, Ralstonia and Pseudomonadaceae were the dominant microorganisms in the biomass degradation systems. It was proved that the combined treatment technology of steam explosion and microbial consortium degradation could overcome the drawbacks of traditional microbial pretreatment technology, and can facilitate the subsequent high-value conversion of lignocellulose.
Clinical Targeted Next-Generation Panel Sequencing Reveals MYC Amplification Is a Poor Prognostic Factor in Osteosarcoma
Authors: Marinoff Amanda E. , Spurr Liam F. , Fong Christina , Li Yvonne Y. , Forrest Suzanne J. , Ward Abigail , Doan Duong , Corson Laura , Mauguen Audrey , Pinto Navin , Maese Luke , Colace Susan , Macy Margaret E. , Kim AeRang , Sabnis Amit J. , Applebaum Mark A. , Laetsch Theodore W. , Glade-Bender Julia , Weiser Daniel A. , Anderson Megan , Crompton Brian D. , Meyers Paul , Zehir Ahmet , MacConaill Laura , Lindeman Neal , Nowak Jonathan A. , Ladanyi Marc , Church Alanna J. , Cherniack Andrew D. , Shukla Neerav , Janeway Katherine A.
Published: March 2023

PURPOSE

Osteosarcoma risk stratification, on the basis of the presence of metastatic disease at diagnosis and histologic response to chemotherapy, has remained unchanged for four decades, does not include genomic features, and has not facilitated treatment advances. We report on the genomic features of advanced osteosarcoma and provide evidence that genomic alterations can be used for risk stratification.

MATERIALS AND METHODS

In a primary analytic patient cohort, 113 tumor and 69 normal samples from 92 patients with high-grade osteosarcoma were sequenced with OncoPanel, a targeted next-generation sequencing assay. In this primary cohort, we assessed the genomic landscape of advanced disease and evaluated the correlation between recurrent genomic events and outcome. We assessed whether prognostic associations identified in the primary cohort were maintained in a validation cohort of 86 patients with localized osteosarcoma tested with MSK-IMPACT.

RESULTS

In the primary cohort, 3-year overall survival (OS) was 65%. Metastatic disease, present in 33% of patients at diagnosis, was associated with poor OS ( P = .04). The most frequently altered genes in the primary cohort were TP 53, RB1, MYC, CCNE1, CCND3, CDKN2A/B, and ATRX. Mutational signature 3 was present in 28% of samples. MYC amplification was associated with a worse 3-year OS in both the primary cohort ( P = .015) and the validation cohort ( P = .012).

CONCLUSION

The most frequently occurring genomic events in advanced osteosarcoma were similar to those described in prior reports. MYC amplification, detected with clinical targeted next-generation sequencing panel tests, is associated with poorer outcomes in two independent cohorts.
Efficacy of Targeted Trials and Signaling Pathway Landscape in Advanced Gastrointestinal Cancers From SCRUM-Japan GI-SCREEN: A Nationwide Genomic Profiling Program
Authors: Nakamura Yoshiaki , Yamashita Riu , Okamoto Wataru , Komatsu Yoshito , Yuki Satoshi , Ueno Makoto , Kato Ken , Taniguchi Hiroya , Kagawa Yoshinori , Denda Tadamichi , Hara Hiroki , Esaki Taito , Moriwaki Toshikazu , Sunakawa Yu , Oki Eiji , Nagashima Fumio , Nishina Tomohiro , Satoh Taroh , Kawakami Hisato , Yamaguchi Kensei , Ohtsubo Koushiro , Kato Takeshi , Horita Yosuke , Tsuji Akihito , Yasui Hisateru , Goto Masahiro , Hamamoto Yasuo , Wakabayashi Masashi , Ikeno Takashi , Shitara Kohei , Bando Hideaki , Tsuchihara Katsuya , Miki Izumi , Ichiki Hiroko , Ohtsu Atsushi , Yoshino Takayuki
Published: March 2023

PURPOSE

Genomic profiling programs have been implemented to apply next-generation sequencing (NGS) for facilitating trial enrollment. SCRUM-Japan GI-SCREEN is a large-scale genomic profiling program in advanced gastrointestinal cancers using a validated genomic assay with the goal of facilitating enrollment in targeted clinical trials, generating real-world data, and performing clinicogenomic analysis for biomarker discovery.

PATIENTS AND METHODS

Genotyping of tumor tissue samples from 5,743 patients with advanced gastrointestinal cancers enrolled in GI-SCREEN was centrally performed with NGS. Patients were enrolled in matched trials of targeted agents affiliated with GI-SCREEN on the basis of genotyping results.

RESULTS

A total of 11 gastrointestinal cancers were included, with colorectal cancer being the most common. The median age ranged from 59 to 70.5 years across cancer types. Patients enrolled after initiation of first-line treatment had significantly longer overall survival (OS) than that before treatment initiation with a median survival time difference of 8.9 months and a hazard ratio (HR) ranging from 0.25 to 0.73 across cancer types, demonstrating an immortal time bias. One hundred and forty-nine patients received matched therapies in clinical trials on the basis of their identified alterations. Among patients with colorectal cancer harboring actionable alterations, the median OS was significantly longer in patients who received matched therapies in trials than in those who did not (HR, 0.52; 95% CI, 0.26 to 1.01; P = .049). Cancer-specific pathway alterations were significantly associated with shorter survival and related to primary resistance to matched trial therapies.

CONCLUSION

Our genomic profiling program led to patient enrollment in targeted clinical trials and improved survival of patients with colorectal cancer who received matched therapies in clinical trials. To avoid immortal time bias, precautions are needed when using data from patients who have undergone NGS testing after initiation of the evaluated treatment line.
What Happens When Employers Can No Longer Discriminate in Job Ads?
Authors: Kuhn Peter , Shen Kailing
Published: March 2023

When employers' explicit gender requests were unexpectedly removed from a Chinese job board overnight, pools of successful applicants became more integrated: women's (men's) share of callbacks to jobs that had requested men (women) rose by 61 (146) percent. The removal “worked” in this sense because it generated a large increase in gender-mismatched applications, and because those applications were treated surprisingly well by employers, suggesting that employers' gender requests often represented relatively weak preferences or outdated stereotypes. The job titles that were integrated by the ban, however, were not the most gendered ones, and were disproportionately lower-wage jobs. (JEL J16, J23, J41, J63, J71, M51, P31)
Biological Therapy for Avascular Necrosis of Femoral Head – A Case Report
Authors: Shankar A Navaladi , Jayakumar Tarun , Pranav N Giri , Jeyaraman Madhan
Published: March 2023

Introduction: Avascular necrosis (AVN) of the femoral head is a type of osteonecrosis with the disruption of blood supply to the head of the femur. Management of AVN femoral head depends on the stage of the disease. In this case report, we elaborated on the biological therapy for bilateral AVN of the femoral head. Case report: A 44-year-old male presented with pain in both hips for 2 years with a history of rest pain in both hips. Radiologically, the patient was diagnosed with bilateral AVN of the femoral head. The patient was offered bone marrow aspirate concentrate (BMAC) in the right femoral head, followed up for 7 years, whereas the adult autologous live cultured osteoblasts in the left femoral head, followed up for 6 years. Conclusion: Biological therapy with differentiated osteoblasts remains a viable option for AVN femoral head when compared with an undifferentiated BMAC cocktail. Keywords: Avascular necrosis, femoral head, cultured osteoblasts, bone marrow.
Collaborative practice research in social work: piloting a model for research and professional learning during COVID-19
Authors: Kong Sui-Ting , Stepanova Evgenia , Thanki Vyomesh
Published: March 2023

Given the quintessentially collaborative nature of social work practice research, many researchers have explored the utility of participatory action research for promoting collaborative learning and knowledge production in social work. As a response to this call for participatory practice research methodology, we developed and piloted ‘collaborative practice research in social work’ in the project, ‘Empowering Social Workers in Challenging Times: Learning from Best Practice during COVID-19’. ‘Collaborative practice research in social work’ is a networked approach to social work participatory practice research, designed to integrate practice wisdom and research evidence to produce useful knowledge for social workers to practise ethically and effectively during COVID-19. This article will present some findings from the evaluation of ‘collaborative practice research in social work’, showing how the reversed sequence of involvement (practitioner researchers first and then academics) in research can enable practitioner-led learning, democratise knowledge production and help validate different types of knowledge in social work practice research. ‘Collaborative practice research in social work’ has demonstrated the need to address alienating academic practices that are not sensitive to the needs of practice or see practice as an afterthought. Findings further suggest the need to better prepare academic researchers to engage with participatory practice research, which can be an emotionally unsettling and unfamiliar research environment.
High-frequency monitoring of Secchi-disk depth in Taihu Lake using Himawari-8/AHI data
Authors: Ding Xiaosong , Gong Fang , Li Jiajia , Zhao Min , Li Hao , Bai Ruofeng , Wang Xiao
Published: March 2023

Diurnal monitoring of the Secchi-disk depth (SDD) of eutrophic lakes is the basic requirement to ensure domestic, industrial, and agricultural water use in surrounding cities. The retrieval of SDD in high frequency and longer observation period is the basic monitoring requirement to guarantee water environmental quality. Taking Lake Taihu as an example, the diurnal high-frequency observation (10 mins) data of the geostationary meteorological satellite sensor AHI/Himawari-8 were examined in this study. The results showed that the AHI normalized water-leaving radiance (Lwn) product derived by the Shortwave-infrared atmospheric correction (SWIR-AC) algorithm was consistent with the in situ data, with determination coefficient (R2) all larger than 0.86 and the mean absolute percentage deviation (MAPD) of 19.76%, 12.83%, 19.03% and 36.46% for the 460 nm, 510 nm, 640 nm and 860 nm bands, respectively. 510 nm and 640 nm bands showed more better consistency with in situ data in Lake Taihu. Therefore, an empirical SDD algorithm was established based on the AHI green (510 nm) and red (640 nm) bands. The SDD algorithm was verified by in situ data showed good performance with R2 of 0.81, RMSE of 5.91 cm, and MAPD of 20.67%. Based on the AHI data and established algorithm, diurnal high-frequency variation of the SDD in the Lake Taihu was investigated and the environmental factor (wind speed, turbidity degree, and photosynthetically active radiance) corresponding to diurnal SDD variation were discussed. This study should be helpful for studying diurnal high-dynamics physical-biogeochemical processes in eutrophication lake waters.
Generation of non-uniformly correlated sources with controllable beam profile by devising its statistics in the spatial frequency domain
Authors: Wu Gaofeng , Liang Jing , Wang Fei , Cai Yangjian
Published: March 2023

We introduce an efficient approach to simultaneously tailor the spatial profile and the degree of coherence (DOC) of partially coherent light by devising its statistical properties in the spatial frequency domain. The relationship between the beam profile and the DOC in the source plane and the correlation function and power spectrum in the spatial frequency domain is analyzed in detail. This approach enables us to generate partially coherent sources with spatially uniform or non-uniform coherence states, and the source profiles are controlled. The condition for switching two coherence states is given through two theoretical examples. Furthermore, we validate our approach in experiment through generating two kinds of spatially non-uniform correlated sources with controllable beam profiles. The experimental results agree well with our theoretical analysis.
The temporal dynamics of the Stroop effect from childhood to young and older adulthood
Authors: Ménétré Eric , Laganaro Marina
Published: March 2023

The processes involved in the Stroop task/effect are thought to involve conflict detection and resolution stages. Little is known about the evolution of these two components over the lifespan. It is well admitted that children and older adults tend to show longer response latencies than young adults. The present study aims at clarifying the rational of such changes from childhood to adulthood and in aging by comparing the impacted cognitive processes across age groups. More precisely, the aim was to clarify if all processes take more time to be executed, hence implying that longer latencies rely mainly on processing speed or if an additional process lengthens the resolution of the conflict in children and/or older adults. To this aim we recorded brain electrical activity using EEG in school-age children, young and older adults while they performed a classic verbal Stroop task. The signal was decomposed in microstate brain networks, and age groups and conditions were compared. Behavioral results evolved following an inverted U-shaped curve. In children, different brain states from the ones observed in adults were highlighted, both in the conflict detection and resolution time-windows. Longer latencies in the incongruent condition were mainly attributed to an overly increased duration of the microstates involved in the conflict resolution time window. In aging, the same microstate maps were reported for both young and older adult groups. The differences in performances between groups could be explained by a disproportionally long conflict detection phase, even compressing the latest stage of response articulation. These results tend to favor a specific immaturity of the brain networks involved coupled with a slowing of the processes in children, while cognitive decline could be mostly explained by a general slowing.
Postnatal care services use by mothers: A comparative study of defaulters versus attendees of postnatal clinics in Enugu
Authors: Onwuka Chidinma Ifechi , Ezugwu Euzebus Chinonye , Obi Samuel Nnamdi , Onwuka Chidozie , Dim Cyril Chukwudi , Chigbu Chibuike , Asimadu Eric , Ezeome Ijeoma Victoria , Okeke Tochukwu Christopher , Iyoke Chukwuemeka Anthony
Published: March 2023

Introduction

Despite much emphasis on the reproductive health of women, maternal mortality is still high, especially in postnatal period.

Objective

To assess the prevalence of postnatal care use and reasons for defaults among mothers attending the child immunization clinics in Enugu, Nigeria.

Methods

This was a cross-sectional comparative study of 400 consecutive nursing mothers who presented at the Institute of Child Health of UNTH and ESUTH, Enugu for Second dose of the Oral Polio Vaccine (OPV2) for their babies at 10 weeks postpartum. Data was collected using Interviewer-administered questionnaire and subsequently analyzed with version 22.0 IBM SPSS software, Chicago, Illinois. A p-value of less than 0.05 was considered as statistically significant.

Result

The prevalence of the 6th week postnatal clinic attendance among the mothers was 59%. The majority of the women (60.6%) who had antenatal care by skilled birth attendants attended postnatal clinic. Unawareness and being healthy were the main reasons for not attending postnatal clinic. Following multivariate analysis, place of antenatal (OR = 2.870, 95% C.I = 1.590–5.180, p < 0.001) and mode of delivery (OR = 0.452, 95% C.I = 0.280–0.728, p = 0.001) were the only significant predictors of postnatal clinic attendance (p < 0.05).

Conclusion

Postnatal clinic attendance by women in Enugu is still suboptimal. The main reason for non-attendance of the 6th week postnatal clinic was lack of awareness. There is need for healthcare professionals to create awareness about the importance of postnatal care and encourage mothers to attend.