bluuebunny/crossref_metadata_2025 huggingface dataset:
An Effective and Safe Sedation Technique Combining Target-Controlled Infusion Pump with Propofol, Intravenous Pentazocine, and Bispectral Index Monitoring for Peroral Double-Balloon Endoscopy
Authors: Kawano Seiji , Okada Hiroyuki , Iwamuro Masaya , Kouno Yoshiyasu , Miura Kou , Inokuchi Toshihiro , Kanzaki Hiromitsu , Hori Keisuke , Harada Keita , Hiraoka Sakiko , Kawahara Yoshiro , Yamamoto Kazuhide
Published: February 2015

Background/Aims: Because peroral double-balloon endoscopy (DBE) is a time-consuming, painful procedure, sedation with analgesics, and/or anesthetics is generally required. The aim of this prospective study was to investigate the safety and efficacy of our sedation protocol for peroral DBE, which consisted of target-controlled infusion (TCI) anesthesia with propofol, an intravenous bolus of pentazocine, and bispectral index (BIS) monitoring. Methods: A total of 34 consecutive patients who underwent DBE by the oral approach were enrolled. Patients were primarily sedated with a continuous infusion of propofol and adjusted in accordance with the BIS levels. The bolus infusion of pentazocine was performed when the propofol infusion was insufficient. The primary outcome measure of this study was to ensure the safety and efficacy of this sedation technique. The secondary purpose was to identify the characteristics of the patient who required the bolus infusion of pentazocine. Results: Five patients (14.7%) required a reduction in the dose of propofol. However, no patient experienced any serious adverse events. All patients (100%) and 80.6% (25/31) of endoscopists answered that the sedation protocol was ‘excellent' or ‘enough' for peroral DBE. Eleven patients (32.3%) required a bolus injection of pentazocine. Age <60 years and a total procedure time of >70 min were significant risk-factors for pentazocine use. Conclusions: A combination of propofol via TCI pump, bolus injection of pentazocine as needed, and BIS monitoring was a safe and effective procedure for peroral DBE. Reasonable satisfaction indices were obtained from both patients and endoscopists. Pentazocine was required for young patients and in cases with longer procedure times.
Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila
Authors: Márkus Róbert , Lerner Zita , Honti Viktor , Csordás Gábor , Zsámboki János , Cinege Gyöngyi , Párducz Árpád , Lukacsovich Tamás , Kurucz Éva , Andó István
Published: February 2015

We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation.
Clozapine Induces Autophagic Cell Death in Non-Small Cell Lung Cancer Cells
Authors: Yin Yu-Chun , Lin Chao-Cheng , Chen Tzu-Ting , Chen Jen-Yeu , Tsai Hui-Ju , Wang Chia-Yu , Chen Shiow-Yi
Published: February 2015

Background/Aims: Previous studies have shown that patients with schizophrenia have a lower incidence of cancer than the general population, and several antipsychotics have been demonstrated to have cytotoxic effects on cancer cells. However, the mechanisms underlying these results remain unclear. The present study aimed to investigate the effect of clozapine, which is often used to treat patients with refractory schizophrenia, on the growth of non-small cell lung carcinoma cell lines and to examine whether autophagy contributes to its effects. Methods: A549 and H1299 cells were treated with clozapine, and cell cytotoxicity, cell cycle and autophagy were then assessed. The autophagy inhibitor bafilomycin A1 and siRNA-targeted Atg7 were used to determine the role of autophagy in the effect of clozapine. Results: Clozapine inhibited A549 and H1299 proliferation and increased p21 and p27 expression levels, leading to cell cycle arrest. Clozapine also induced a high level of autophagy, but not apoptosis, in both cell lines, and the growth inhibitory effect of clozapine was blunted by treatment with the autophagy inhibitor bafilomycin A1 or with an siRNA targeting atg7. Conclusions: Clozapine inhibits cell proliferation by inducing autophagic cell death in two non-small cell lung carcinoma cell lines. These findings may provide insights into the relationship between clozapine use and the lower incidence of lung cancer among patients with schizophrenia.
GOLPH3 Overexpression is Closely Correlated with Poor Prognosis in Human Non-Small Cell Lung Cancer and Mediates its Metastasis Through Upregulating MMP-2 and MMP-9
Authors: Wang Ran , Ke Zun-fu , Wang Fen , Zhang Wen-hui , Wang Yue-feng , Li Shu-hua , Wang Lian-tang
Published: February 2015

Background/Aims: Golgi phosphoprotein 3 (GOLPH3) is a newly reported oncogene that plays a significant role in regulating cell growth. Recent research has shown that overexpression of GOLPH3 is correlated with patient survival and M classification in breast cancer and other cancers. However, the mechanisms by which GOLPH3 contributes to metastasis in non-small cell lung cancer (NSCLC) have not been previously clarified and are therefore the focus of this work. Methods: Immunohistochemistry (IHC) and western blotting analysis were performed to assess the GOLPH3 protein level, small interfering RNA (siRNA) and transwell assays were conducted to investigate the role of GOLPH3 in migration and invasion, and real-time PCR was performed to estimate the level of GOLPH3 mRNA expression. Results: GOLPH3 was significantly correlated with clinicopathological variables, such as the clinical stage (P=0.012), T classification (P=0.002) and metastasis (M classification) (P=0.008), in NSCLC patients and was negatively correlated with the prognosis. Knockdown of GOLPH3 significantly suppressed the migratory and invasive ability of NSCLC cell lines and downregulated the enzyme activity and protein levels of MMP-2 and MMP-9. Conclusions: The expression level of GOLPH3 is correlated with metastasis and prognosis in NSCLC, and GOLPH3 mediates metastasis by regulating the protein levels of MMP-2 and MMP-9 in vitro.
MACC1 Suppresses Cell Apoptosis in Hepatocellular Carcinoma by Targeting the HGF/c-MET/AKT Pathway
Authors: Yao Yingmin , Dou Chanwei , Lu Zhongtang , Zheng Xin , Liu Qingguang
Published: February 2015

Background & Aims: To investigate the expression and prognostic value of MACC1 in patients with HCC and identify the mechanism by which MACC1 inhibits HCC cell apoptosis. Methods: MACC1 and p-AKT expression was studied using immunohistochemistry of both HCC tissues and adjacent liver tissues. qRT-PCR and western immunoblotting were used to examine the expression of target genes at the mRNA and protein levels, respectively. The MTT assay was used to assess cell viability, and cell apoptosis was determined by DAPI staining, Annexin V/PI staining and Caspase 3/7 assay. Nude mice were used to perform in vivo experiments. Results: The overexpression of MACC1 was found in HCC tissues and was correlated with poor postsurgical prognosis. There was a positive relationship between MACC1 and p-AKT expression in HCC tissues. In vitro experiments showed that MACC1 repressed HCC cell apoptosis and promoted cell growth. Knockdown of c-MET abolished the anti-apoptotic function of MACC1. Next, MACC1 was verified to activate PI3K/AKT signaling by sensitizing HGF/c-MET signaling in HCC. MACC1 overexpression enhanced the HGF-driven phosphorylation of BAD, Caspase 9 and FKHRL1 and inhibited their pro-apoptotic functions in HCC cells. Finally, MACC1 was shown to inhibit cell apoptosis and promote HCC growth in vivo. Conclusions: This investigation revealed that MACC1 overexpression predicted worse prognosis after liver resection, which was attributed to the repression of HCC cell apoptosis via a molecular mechanism in which MACC1 accelerated the activation of the HGF/c-MET/PI3K/AKT pathway and phosphorylated BAD, Caspase 9 and FKHRL1, ultimately preventing their nuclear translocation and their pro-apoptotic function.
Inadequate Cortisol Response to the Tetracosactide (Synacthen®) Test in Non-Classic Congenital Adrenal Hyperplasia: An Exception to the Rule?
Authors: Stoupa Athanasia , González-Briceño Laura , Pinto Graziella , Samara-Boustani Dinane , Thalassinos Caroline , Flechtner Isabelle , Beltrand Jacques , Bidet Maud , Simon Albane , Piketty Marie , Laborde Kathleen , Morel Yves , Bellanné-Chantelot Christine , Touraine Philippe , Polak Michel
Published: February 2015

Aims: To describe cortisol response to tetracosactide and to review the literature on adrenal function in non-classic congenital adrenal hyperplasia (NCCAH) patients. Methods: We compared cortisol responses to tetracosactide (250 μg) between NCCAH patients and a comparison group (CG) of patients with premature pubarche and normal tetracosactide test. An adequate cortisol response was defined as a peak ≥18 μg/dl. Results: We included 35 NCCAH patients (26 girls, 9 boys), whose mean age at testing was 7.0 years (0.8-15.6), and 47 patients in the CG (39 girls, 8 boys), whose mean age was 7.2 years (0.5-9.9). Baseline cortisol was significantly higher in the NCCAH group than in the CG [12.9 (4.3-22.2) vs. 9.7 (4.2-16.2) μg/dl, respectively; p = 0.0006]. NCCAH patients had lower cortisol peak response compared to the CG [18.2 (6.3-40) vs. 24.9 (12-30.3) μg/dl, respectively; p < 0.0001]. Peak cortisol was <18 μg/dl in 21/35 (60%) NCCAH patients versus 1/47 (2.1%) in the CG. No NCCAH patients had acute adrenal insufficiency, but 2 reported severe fatigue that improved with hydrocortisone. Conclusions: The cortisol response to tetracosactide was inadequate (<18 μg/dl) in 60% of patients with NCCAH. Hydrocortisone therapy may deserve consideration when major stress (surgery, trauma, childbirth) or objectively documented fatigue occurs in NCCAH patients with inadequate cortisol response.
Blood Outgrowth Endothelial Cells Reduce Hypoxia-Mediated Fibroblast to Myofibroblast Conversion by Decreasing Proangiogenic Cytokines
Authors: Nieves Torres Evelyn C. , Yang Binxia , Brahmbhatt Akshaar , Mukhopadhyay Debabrata , Misra Sanjay
Published: February 2015

Purpose: Hypoxic conditions cause fibroblasts to differentiate into alpha smooth-muscle cell actin (α -SMA)-positive cells, i.e. myofibroblasts. This process is a hallmark of venous neointimal hyperplasia (VNH) associated with hemodialysis vascular access. The purpose of this study was to determine if blood outgrowth endothelial cells (BOEC) may reduce the conversion of fibroblasts into myofibroblasts under hypoxic conditions, and to determine the potential mechanisms involved. Methods: An experimental model was used, in which fibroblasts and BOEC were subjected to hypoxia under contact and transwell conditions to determine if BOEC reduce the conversion of fibroblasts into myofibroblasts under hypoxic conditions. Gene expression under different conditions was performed. In addition, functional assays including cell proliferation and migration were determined. Results: This study demonstrates that contact needs to occur between BOEC and fibroblasts for the reduction of the hypoxia-driven conversion of fibroblasts into α-SMA. This is associated with a decrease in several proangiogenic genes including vascular endothelial growth factor A, platelet-derived growth factor, fibroblast growth factor and matrix metalloproteinase 2 in fibroblasts in contact with BOEC when compared to fibroblasts alone. In addition, migration is significantly reduced while proliferation remains unchanged. Conclusion: This study helps provide rationale for using BOEC delivered to the adventitia of the outflow vein of hemodialysis vascular access to reduce VNH.
A Comprehensive Whole-Genome Integrated Cytogenetic Map for the Alpaca (Lama pacos)
Authors: Avila Felipe , Baily Malorie P. , Perelman Polina , Das Pranab J. , Pontius Joan , Chowdhary Renuka , Owens Elaine , Johnson Warren E. , Merriwether David A. , Raudsepp Terje
Published: February 2015

Genome analysis of the alpaca (Lama pacos, LPA) has progressed slowly compared to other domestic species. Here, we report the development of the first comprehensive whole-genome integrated cytogenetic map for the alpaca using fluorescence in situ hybridization (FISH) and CHORI-246 BAC library clones. The map is comprised of 230 linearly ordered markers distributed among all 36 alpaca autosomes and the sex chromosomes. For the first time, markers were assigned to LPA14, 21, 22, 28, and 36. Additionally, 86 genes from 15 alpaca chromosomes were mapped in the dromedary camel (Camelus dromedarius, CDR), demonstrating exceptional synteny and linkage conservation between the 2 camelid genomes. Cytogenetic mapping of 191 protein-coding genes improved and refined the known Zoo-FISH homologies between camelids and humans: we discovered new homologous synteny blocks (HSBs) corresponding to HSA1-LPA/CDR11, HSA4-LPA/CDR31 and HSA7-LPA/CDR36, and revised the location of breakpoints for others. Overall, gene mapping was in good agreement with the Zoo-FISH and revealed remarkable evolutionary conservation of gene order within many human-camelid HSBs. Most importantly, 91 FISH-mapped markers effectively integrated the alpaca whole-genome sequence and the radiation hybrid maps with physical chromosomes, thus facilitating the improvement of the sequence assembly and the discovery of genes of biological importance.
Immunological Aspects of the Immune Response Induced by Mosquito Allergens
Authors: Cantillo José Fernando , Fernández-Caldas Enrique , Puerta Leonardo
Published: February 2015

Allergies caused by mosquito bites may produce local or systemic reactions. The inhalation of mosquito allergens may also cause asthma and/or allergic rhinoconjunctivitis in sensitized individuals. The mechanisms implicated in the development of these immune responses involve IgE antibodies, different subtypes of IgG and proinflammatory cytokines as well as basophils, eosinophils and mast cells. Several allergenic components have been identified in the saliva and bodies of mosquitoes and some of these are present in different mosquito species. The most common species implicated in allergic reactions belong to the genera Aedes, Culex and Anopheles. Several Aedes aegypti allergens have been cloned and sequenced. The recombinant molecules show IgE reactivity similar to that of the native allergens, making them good candidates for the diagnosis of mosquito allergies. Allergen-specific immunotherapy with mosquito extracts induces a protective response characterized by a decreased production of IgE antibodies, increased IgG levels, a reduction in the severity of cutaneous and respiratory symptoms and the need for medication. The aims of this review are to summarize the progress made in the characterization of mosquito allergens and discuss the types of immune responses induced by mosquito bites and the inhalation of mosquito allergens in atopic individuals.
C4a: An Anaphylatoxin in Name Only
Authors: Barnum Scott R.
Published: February 2015

Activation of complement leads to generation of the 3 anaphylatoxins C3a, C4a, and C5a. Although all 3 peptides are structurally similar, only C3a and C5a share a similar functional profile that includes the classic inflammatory activities and, more recently, developmental homing and regenerative properties among others. In contrast, the functional profile of C4a is questionable in most cases owing to contamination of C4a preparations with physiologically relevant levels of C3a and/or C5a. Combined with the absence of an identified C4a receptor and the inability of C4a to signal through the C3a and C5a receptors, it is clear that C4a should not be included in the family of complement anaphylatoxins.
Erweiterung der kognitiven Verhaltenstherapie um Emotionsregulationstraining bei Patienten mit multiplen somatoformen Symptomen: Ergebnisse einer kontrollierten Pilotstudie
Authors: Gottschalk Japhia-Marie , Bleichhardt Gaby , Kleinstäuber Maria , Berking Matthias , Rief Winfried
Published: February 2015

Hintergrund: Kognitive Verhaltenstherapie (KVT) ist bei Patienten mit multiplen somatoformen Symptomen (MSS) erwiesenermaßen wirksam, bisher jedoch nur mit moderaten Effekten. Da sich bei diesen Patienten Hinweise für ein Emotionswahrnehmungsdefizit und Regulationsdefizit finden, wurde an der Universität Marburg ein Behandlungsmanual für Patienten mit MSS entwickelt, das KVT-Elemente und ein spezielles Emotionsregulationstraining verbindet. In diesem soll der Umgang mit Emotionen durch Strategien der bewertungsfreien Wahrnehmung, Akzeptanz und Achtsamkeit gefördert werden. Ziel der vorliegenden Studie war, die Durchführbarkeit des Behandlungsprogramms ENCERT (Enriching CBT with Emotion Regulation Training) zu testen sowie die Veränderungen über die Zeit unter ENCERT mit denen einer klassischen KVT zu vergleichen. Patienten und Methoden: 20 ENCERT-Patienten und 22 KVT-Patienten (mit ≥3 somatoformen Symptomen, seit ≥6 Monaten) nahmen im Rahmen einer nicht randomisierten Gruppenvergleichsstudie an 20 wöchentlichen Einzeltherapiesitzungen im ambulanten Setting teil. Zu Therapiebeginn und -ende wurden relevante Daten erhoben. Die primären Outcomemaße waren das Screening für somatoforme Störungen (SOMS-7T), der Fragebogen zur Selbsteinschätzung emotionaler Kompetenzen (SEK-27) und visuelle Analogskalen (VAS) zur Einschätzung der Symptomintensität und zur empfundenen Beeinträchtigung. Ergebnisse: Beide Gruppen verbesserten sich im Therapieverlauf signifikant bezüglich der Beeinträchtigungsintensität (ENCERT d = 0,70; KVT d = 0,46) und Beschwerdeanzahl (ENCERT d = 0,72; KVT d = 0,50). Gruppenunterschiede zu Therapieende zeigten sich nicht: Unter Berücksichtigung klinisch relevanter Baselinevariablen zeigte sich zu Therapieende ein Hinweis auf einen signifikanten Unterschied in der Beeinträchtigungsintensität (ANCOVA: F (1,37) = 4,058, p = 0,051; ENCERT überlegen). ENCERT-Patienten verbesserten sich zudem signifikant auf stündlich erhobenen visuellen Analogskalen (Beschwerdeintensität d = 0,59; Beeinträchtigung durch Beschwerden d = 1,25) und in ihren Emotionsregulationskompetenzen (d = 1,14). Schlussfolgerung: Patienten mit MSS verbesserten sich während ENCERT und KVT, mit tendenziell höheren Effektstärken für ENCERT, jedoch auch höheren Ausgangswerten in Depressivität und Angst. Gruppenunterschiede zeigten sich nicht, möglicherweise aufgrund geringer Power. Diese Ergebnisse ermutigen zur Durchführung einer größeren randomisierten und kontrollierten Multicenterstudie.
Prevalence of Age-Related Macular Degeneration in Portugal: The Coimbra Eye Study - Report 1
Authors: Cachulo Maria da Luz , Lobo Conceição , Figueira João , Ribeiro Luisa , Laíns Inês , Vieira António , Nunes Sandrina , Costa Miguel , Simão Sílvia , Rodrigues Victor , Vilhena Nelson , Cunha-Vaz José , Silva Rufino
Published: February 2015

Purpose: To evaluate the age- and gender-specific prevalence of early and late age-related macular degeneration (AMD) in a Portuguese population-based sample. Methods: All patients aged ≥55 years of a Portuguese primary health-care unit were recruited for a cross-sectional population-based study. Responders underwent complete ophthalmological examination and digital fundus imaging. Early and late AMD was defined according to the International Age-Related Macular Epidemiological Study Group Classification, and the adopted staging for AMD was the same as that used in the Rotterdam study. The age- and gender-adjusted prevalence of early and late forms of AMD was calculated. Results: Of the 4,370 eligible subjects, 3,000 underwent study procedures (68.6% response rate) and 2,975 were included in the analysis; they had a mean age of 68.9 ± 8.6 years. The overall prevalence of early and late AMD was 15.53% (95% CI 14.25-16.88) and 0.67% (95% CI 0.41-1.04), respectively. Neovascular AMD (NV-AMD) and geographic atrophy (GA) accounted for 0.44% (95% CI 0.23-0.75) and 0.27% (95% CI 0.12-0.53) of individuals, respectively. The highest prevalence of advanced AMD was among those aged ≥75 years (1.13% for NV-AMD; 0.63% for GA). Conclusions: To our knowledge, this is the first AMD epidemiological study in a Portuguese population. The early forms of the disease had a similar prevalence to that of other large-scale population-based cohorts, but late AMD was less frequent than previously reported.
Dexamethasone Implants in Patients with Naïve or Refractory Diffuse Diabetic Macular Edema
Authors: Escobar-Barranco José Juan , Pina-Marín Begoña , Fernández-Bonet Manel
Published: February 2015

Purpose: To assess the effect of dexamethasone (DEX) 0.7 mg (Ozurdex™) on refractory and treatment-naïve diffuse diabetic macular edema. Methods: A prospective study was conducted in 76 patients (40 refractory and 36 naïve) with visual acuities (VA) of 15-72 ETDRS letters, central macular thickness (CMT) >300 µm and intraocular pressure (IOP) <25 mm Hg. After the DEX implant (±photocoagulation), VA, CMT and total macular volume (TMV) were assessed monthly for 6 months. Results: At every visit, VA improved significantly from baseline (p < 0.001) in both groups, but values were significantly better in the naïve group, while CMT and TMV decreased significantly (p < 0.001) and similarly in both groups. The naïve group received more photocoagulations (p = 0.001). There were 7.9% transient IOP increases >10 mm Hg. Conclusions: Substantial improvements in VA and CMT were achieved in both groups, and a gain of 1 ETDRS line was observed in the treatment-naïve group as compared to the refractory group, with a good safety profile in both.
Microglia are Involved in Pruritus Induced by DNFB via the CX3CR1/p38 MAPK Pathway
Authors: Zhang Ying , Yan Jia , Hu Rong , Sun Yu , Ma Yiwen , Chen Zhifeng , Jiang Hong
Published: February 2015

Background/Aims: Pruritus, also known as itch, is a common, unpleasant sensation that can be difficult to treat. Frequently, chronic itch is associated with the development of neuropathic pain resulting from nerve injury or insult. Previous studies have shown the involvement of spinal microglia in the development of neuropathic pain, but their role in chronic pruritus is unclear. Methods: For this study, we constructed a model of chronic pruritus in mice using repeated applications of 2, 4-dinitrofluorobenzene (DNFB) and showed prolonged scratching behavior in treated mice that continued for at least 7 d after the final DNFB treatment. Results: Scratching was accompanied by activation of spinal microglia and both were reduced by an inhibitor of microglial activity. We also showed that microglial activation entailed increased signaling in the p38 MAPK pathway, and treatment with a p38 inhibitor reduced scratching in DNFB-treated mice. We also examined the role of fractalkine/CX3CR1 signaling in the development of DNFB-induced pruritus and showed that intrathecal administration of antiserum against either CX3CR1or FKN inhibited p38 activity and decreased scratching. Conclusion: Our results suggest that microglia are involved in pruritus induced by DNFB via FKN/CX3CR1/p38MAPK pathways similar to those participating in the development of neuropathic pain.
Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments
Authors: Tauber Svantje , Hauschild Swantje , Paulsen Katrin , Gutewort Annett , Raig Christiane , Hürlimann Eva , Biskup Josefine , Philpot Claudia , Lier Hartwin , Engelmann Frank , Pantaleo Antonella , Cogoli Augusto , Pippia Proto , Layer Liliana E. , Thiel Cora S. , Ullrich Oliver
Published: February 2015

Background/Aims: Several limiting factors for human health and performance in microgravity have been clearly identified arising from the immune system, and substantial research activities are required in order to provide the basic information for appropriate integrated risk management. The gravity-sensitive nature of cells of the immune system renders them an ideal biological model in search for general gravity-sensitive mechanisms and to understand how the architecture and function of human cells is related to the gravitational force and therefore adapted to life on Earth. Methods: We investigated the influence of altered gravity in parabolic flight and 2D clinostat experiments on key proteins of activation and signaling in primary T lymphocytes. We quantified components of the signaling cascade 1.) in non-activated T lymphocytes to assess the “basal status” of the cascade and 2.) in the process of activation to assess the signal transduction. Results: We found a rapid decrease of CD3 and IL-2R surface expression and reduced p-LAT after 20 seconds of altered gravity in non-activated primary T lymphocytes during parabolic flight. Furthermore, we observed decreased CD3 surface expression, reduced ZAP-70 abundance and increased histone H3-acetylation in activated T lymphocytes after 5 minutes of clinorotation and a transient downregulation of CD3 and stable downregulation of IL-2R during 60 minutes of clinorotation. Conclusion: CD3 and IL-2R are downregulated in primary T lymphocytes in altered gravity. We assume that a gravity condition around 1g is required for the expression of key surface receptors and appropriate regulation of signal molecules in T lymphocytes.
Rosiglitazone Promotes AQP2 Plasma Membrane Expression In Renal Cells Via a Ca2+-Dependent/cAMP-Independent Mechanism
Authors: Procino Giuseppe , Gerbino Andrea , Milano Serena , Nicoletti Maria Celeste , Mastrofrancesco Lisa , Carmosino Monica , Svelto Maria
Published: February 2015

Background/Aims: Thiazolidinediones are highly beneficial in the treatment of type II diabetes. However, they are also associated with edema and increased risk of congestive heart failure. Several studies demonstrated that rosiglitazone (RGZ) increases the abundance of aquaporin-2 (AQP2) at the plasma membrane of renal cells. The aim of this study was to investigate whether RGZ might activate a transduction pathway facilitating AQP2 membrane accumulation in renal cells. Methods: We analyzed the effect of RGZ on renal AQP2 intracellular trafficking in MCD4 renal cells by confocal microscopy and apical surface biotinylation. Cytosolic Ca2+ dynamics were measured by a video-imaging approach in single cell. Transient Receptor Potential (TRP) channels expression was determined by RT-PCR. Results: We showed that in MCD4 cells, short-term exposure to RGZ dramatically increases the amount of apically expressed AQP2 independently on cAMP production, PKA activation and AQP2 phosphorylation. RGZ elicited a cytosolic Ca2+ transient due to Ca2+ influx prevented by ruthenium red, suggesting the involvement of TRP plasma membrane channels. We identified TRPV6 as the possible candidate mediating this effect. Conclusions: Taken together these results provide a possible molecular mechanism explaining the increased AQP2 membrane expression under RGZ treatment: in renal cells RGZ elicits Ca2+ transients facilitating AQP2 exposure at the apical plasma membrane, thus increasing collecting duct water permeability. Importantly, this effect suggests an unexplored application of RGZ in the treatment of pathological states characterized by impaired AQP2 trafficking at the plasma membrane.
Role of Serum miRNAs in the Prediction of Ovarian Hyperstimulation Syndrome in Polycystic Ovarian Syndrome Patients
Authors: Zhao Chun , Liu Xiaoguang , Shi Zhonghua , Zhang Jing , Zhang Junqiang , Jia Xuemei , Ling Xiufeng
Published: February 2015

Background: Polycystic ovarian syndrome (PCOS) causes a significantly increased risk of ovarian hyperstimulation syndrome (OHSS). Here, we focused on the altered expression of serum miRNAs and their predictive value for OHSS in PCOS patients. Methods: We used the TaqMan low density array followed by individual quantitative reverse transcription-polymerase chain reaction to identify and validate the expression of serum miRNAs in PCOS patients likely to develop severe OHSS. Results: The miR-16 and miR-223 expression levels were significantly reduced in the patients who were likely to develop severe OHSS than in the control subjects who were likely to develop mild or no OHSS. The sensitivity and specificity of the basal LH, basal LH/FSH, and body mass index (BMI) as OHSS predictors were also evaluated. miR-16 was the most efficient for OHSS prediction as it yielded the highest AUC. Logistic binary regression analyses revealed a positive association of miR-223 and BMI. Conclusion: Serum miRNAs are differentially expressed in PCOS patients likely to suffer from severe OHSS. We identified and validated two serum miRNAs that have potential for use as novel noninvasive biomarkers to accurately predict OHSS before controlled ovarian hyperstimulation (COH) for PCOS patients.
The Future of Cognitive Behavioral Therapy
Authors: Hofmann Stefan
Published: February 2015

Cognitive behavioral therapy is an effective treatment for virtually all psychiatric disorders. However, only few patients receive adequate treatment in most countries around the word. One of the few exceptions is UK and its program Improving Access to Psychological Therapies (IAPT), which clearly demonstrates the enormous cost-savings of training and disseminating CBT. Another important avenue for the future of CBT is to move toward personalized care, which may only be achieved by departing from the psychiatric classification system and the medical model. Current obstacles for achieving these goals are discussed.
Transient Tropical Diabatic Heating and the Seasonal-Mean Response to ENSO
Authors: Swenson Erik T. , Straus David M.
Published: February 2015

Boreal winter simulations of the Community Atmospheric Model, version 4.0, were carried out using observed sea surface temperature (SST) fields from the three El Niño events of 1982/83, 1991/92, and 1997/98 [control (CTL) runs] and from observed climatology (CLIM run). In each case, 50 ensemble members were run (1 November–31 March). The diabatic heating Q at every grid point, level, and day of the CTL runs in the Indo-Pacific region was stored and used in four additional suites of experiments, each of which parallels the appropriate CTL suite. In each suite, Q generated by the model is replaced by a specified subset of Q at every time step, grid point, and level spanning the Indo-Pacific. The Q subsets consist of the seasonal ensemble-CTL-mean Q for each ensemble member (suite FIX), the seasonal-mean Q from the appropriate ensemble member of the CTL (suite EFIX), the seasonal mean plus low-frequency component of Q (suite ESUBFIX), and the daily means of Q (suite DAYFIX).

The midlatitude ENSO anomalies of the seasonal-mean upper-level height field and time-filtered meridional wind variance are enhanced in the FIX, EFIX, and ESUBFIX suites, with little change in patterns, compared to CTL anomalies. The enhancements have a smaller magnitude in ESUBFIX and especially in DAYFIX; qualitative differences are seen in DAYFIX. These differences are due to (i) the required setup time for midlatitude response, (ii) the altered relationship between vertical structure and vertically integrated heating, and (iii) the lack of midlatitude interactive influence on tropical heating in the experiments.
Systematic Comparison of Four-Dimensional Data Assimilation Methods With and Without the Tangent Linear Model Using Hybrid Background Error Covariance: E4DVar versus 4DEnVar
Authors: Poterjoy Jonathan , Zhang Fuqing
Published: February 2015

Two ensemble formulations of the four-dimensional variational (4DVar) data assimilation technique are examined for a low-dimensional dynamical system. The first method, denoted E4DVar, uses tangent linear and adjoint model operators to minimize a cost function in the same manner as the traditional 4DVar data assimilation system. The second method, denoted 4DEnVar, uses an ensemble of nonlinear model trajectories to replace the function of linearized models in 4DVar, thus improving the parallelization of the data assimilation. Background errors for each algorithm are represented using a hybrid error covariance, which includes climatological errors as well as ensemble-estimated errors from an ensemble Kalman filter (EnKF). Numerical experiments performed over a range of scenarios suggest that both methods provide similar analysis accuracy for dense observation networks, and in perfect model experiments with large ensembles. Nevertheless, E4DVar has clear benefits over 4DEnVar when substantial covariance localization is required to treat sampling error. The greatest advantage of the tangent-linear approach is that it implicitly propagates a localized, full-rank ensemble covariance in time, thus avoiding the need to localize a time-dependent ensemble covariance. The tangent linear and adjoint model operators also provide a means of evolving flow-dependent information from the climate-based error component, which is found to be beneficial for treating model error. Challenges that need to be overcome before adopting a pure ensemble framework are illustrated through experiments estimating time covariances with four-dimensional ensembles and comparing results with those estimated with a tangent linear model.
The Content and Effect of Politicized Health Controversies
Authors: Fowler Erika Franklin , Gollust Sarah E.
Published: February 2015

Health issues are increasingly becoming politicized, but little is known about how politicization takes shape in the news and its effect on the public. We analyze the evolution of politicization in news coverage of two health controversies: the uproar over the 2009 mammography screening guidelines and the 2006–2007 debate over mandating the HPV vaccine as a requirement for middle school–aged girls. We then examine the public response to politicization in the HPV case, using original data from a survey-embedded experiment that was linked with news coverage in all fifty states. We find that real-world politicization is associated with decreases in support for HPV vaccine requirements, state immunization programs, and confidence in doctors and in government. In addition, among those less likely to have encountered real-world politicization, we find marginal evidence that exposure to political conflict decreases support for state immunization programs and clear evidence that politicization reduces confidence in doctors. We discuss the implications of these findings and suggest future avenues of research.
Selecting Our Own Science
Authors: Yeo Sara K. , Xenos Michael A. , Brossard Dominique , Scheufele Dietram A.
Published: February 2015

We use an experiment with a nationally representative sample of the U.S. population to examine how political partisans consume and process media reports about nanotechnology—a scientific issue that is unfamiliar to most Americans. We manipulate the extent to which participants receive ideological cues contextualizing a news article, and follow their subsequent information seeking about nanotechnology. Our results provide insights into patterns of media use and how media use differs among people with varying political ideologies. When cues clarifying the political stakes of nanotechnology are made available, individuals are willing to read information from countervailing sources. When such cues are lacking, however, individuals avoid incongruent information and opt for headlines from attitude-consistent sources. We explore variations in the circumstances under which ideological selectivity occurs and demonstrate that both confirmation bias and defensive avoidance are heightened under such conditions.
Citizens’, Scientists’, and Policy Advisors’ Beliefs about Global Warming
Authors: Bolsen Toby , Druckman James N. , Cook Fay Lomax
Published: February 2015

Numerous factors shape citizens’ beliefs about global warming, but there is very little research that compares the views of the public with key actors in the policymaking process. We analyze data from simultaneous and parallel surveys of (1) the U.S. public, (2) scientists who actively publish research on energy technologies in the United States, and (3) congressional policy advisors and find that beliefs about global warming vary markedly among them. Scientists and policy advisors are more likely than the public to express a belief in the existence and anthropogenic nature of global warming. We also find ideological polarization about global warming in all three groups, although scientists are less polarized than the public and policy advisors over whether global warming is actually occurring. Alarmingly, there is evidence that the ideological divide about global warming gets significantly larger according to respondents’ knowledge about politics, energy, and science.
Geoengineering and Climate Change Polarization
Authors: Kahan Dan M. , Jenkins-Smith Hank , Tarantola Tor , Silva Carol L. , Braman Donald
Published: February 2015

The cultural cognition thesis posits that individuals rely extensively on cultural meanings in forming perceptions of risk. The logic of the cultural cognition thesis suggests that a two-channel science communication strategy, combining information content (“Channel 1”) with cultural meanings (“Channel 2”), could promote open-minded assessment of information across diverse communities. We test this kind of communication strategy in a two-nation (United States, n = 1,500; England, n = 1,500) study, in which scientific information content on climate change was held constant while the cultural meaning of that information was experimentally manipulated. We found that cultural polarization over the validity of climate change science is offset by making citizens aware of the potential contribution of geoengineering as a supplement to restriction of CO2 emissions. We also tested the hypothesis, derived from a competing model of science communication, that exposure to information on geoengineering would lead citizens to discount climate change risks generally. Contrary to this hypothesis, we found that subjects exposed to information about geoengineering were slightly more concerned about climate change risks than those assigned to a control condition.
The Macroeconomic Record of the Coalition Government
Authors: Wren-Lewis Simon
Published: February 2015

This paper examines the outcomes for changes introduced by the UK Coalition government in 2010. The Office for Budget Responsibility (OBR) is generally regarded as a success, and should become a permanent part of fiscal policymaking. The form of the primary fiscal mandate, involving a five-year rolling target, appears to be a sensible way to shape fiscal decisions when monetary policy is able to stabilise the economy. Unfortunately it was introduced, along with a five-year programme of severe fiscal consolidation (austerity), while the economy was in a liquidity trap. The OBR estimates austerity reduced GDP growth by 1 per cent in both 2010–11 and 2011–12, and monetary policy was unable to offset this. For the Liberal Democrats a misreading of the Eurozone crisis may have been responsible for this mistake, but for the Conservatives this mistake appears to derive from an unconventional view that the liquidity trap is unimportant.
Key Articles and Guidelines in the Management of Hypertension
Authors: Ripley Toni L. , Brenner Michael , Finks Shannon , Hough Augustus , McConnell Karen J. , Parker Mary , Dobesh Paul P.
Published: February 2015

Hypertension is a major risk factor for cardiovascular disease. Evidence for optimal pharmacotherapy continues to accumulate at a very rapid pace; maintaining an up-to-date library of key articles for hypertension management can be challenging for busy clinicians. Further, there has been controversy surrounding the hypertension guidelines that were released in late 2013 and early 2014. The lack of congruence and simplicity in the current hypertension recommendations could result in delays with application of evidence to clinical practice. In order to facilitate clinicians’ efficient access to high-impact clinical trials evaluating the management of hypertension, this compilation of annotated bibliographies was created to serve as a resource for any health care professional participating in the management of adult patients with hypertension.
Pedagogical stylistics in multiple foreign language and second language contexts: A synthesis of empirical research
Authors: Fogal Gary G
Published: February 2015

This article examines the efficacy of pedagogical stylistics as a learning tool for developing second or foreign language proficiency. Pedagogical stylistics – an instrument for investigating the linguistic, sociocultural and dialogic features inherent in literary and non-literary texts – has often been criticized for relying too heavily on intuition rather than empirical support to substantiate its employment in language learning classrooms. To better understand this criticism a coding framework adapted from previous research was employed to synthesize 13 studies across four, second or foreign languages in nine countries. Three themes emerged from this synthesis: (1) stylistics as a tool for improving L2 performance; (2) stylistics’ contribution to building language awareness; (3) stylistics as a tool for building academic skills beyond L2 acquisition. This work explores these themes and discusses the research practices informing the claims made therein, highlighting a consistent underreporting or under collecting of data as a recurring problem in the literature. This shortcoming precludes a meta-analysis of the literature, and this article argues that this shortcoming contributes to a justifiably weak representation of stylistics in second or foreign language contexts. To rectify this issue suggestions are made for more thorough reporting of data and a more robust research agenda in second or foreign language-based, stylistic contexts.
Latin American Economic Growth
Authors: Edmunds John C. , Chartier Frederic
Published: February 2015

Seven major Latin American countries had moderately high growth rates during the period 2001–2012.We used indicators of capital market modernization and corruption to explain the widely varying GDP growth figures for each time frame and also assessed whether export growth was highly linked to GDP growth. The countries did not fall into clusters with each coinciding closely with the others in the cluster. Instead each country appeared to be sui generis, finding its own path in response to its own circumstances.
Social Workers’ Training Evaluated by a Cluster-Randomized Study
Authors: Malmberg-Heimonen Ira Elena
Published: February 2015

Objective:

Using a cluster-randomized design, this study evaluates whether a skill training program for social workers increases reemployment among welfare recipients. The program focuses on systematizing follow-up work within the areas of encounters with users, system-oriented efforts, and administrative work.

Methods:

Data consist of baseline and 18-month follow-up questionnaires for welfare recipients ( N = 617) in the 18 participating labor and welfare offices randomized into experimental and control groups. Full-time employment, part-time employment, and participation in active labor market programs were assessed.

Results:

After adjusting for the clustered structure of data, the findings demonstrate that social workers’ skill training increased reemployment among welfare recipients. Although neutral effects on full-time employment, there was a highly significant effect on part-time employment ( p = .000). In contrast, recipients from the control group were significantly more often in activation programs at the follow-up ( p = .004).

Conclusions:

Close and comprehensive follow-ups to support reemployment among welfare recipients should be emphasized.
Social Work and Science
Authors: Gehlert Sarah
Published: February 2015

Interest has grown in the past few years about the place of social work in science. Questions remain, such as whether social work should be considered a science, and if so, where it fits into the constellation of sciences. This article attempts to shed light on these questions. After briefly considering past and present constructions of science and reflecting on views of science within the social work profession over time, we present an argument for how social work contributes to predominant questions facing science today through its unique ability to draw together and integrate knowledge from a variety of disciplines. Finally, we address how the profession can best prepare the coming generation of social workers to operate to their full potential in the current transdisciplinary world of science.
The Year in Thoracic Surgery
Authors: Weyant Michael J.
Published: February 2015

The year 2014 produced several noteworthy articles that have advanced the care of thoracic surgery patients. Further knowledge has been gained regarding screening and treatment of early stage lung cancer. The use of neoadjuvant therapy for subsets of patients with esophageal cancer continues to evolve. Endobronchial therapy for emphysema is reported in a multicenter trial. The purpose of this review is to highlight the major advances in thoracic surgical disease in the year 2014.
The Intersection of Victim Race and Gender
Authors: Girgenti Alicia A.
Published: February 2015

Previous research on race and the death penalty has shown that victim race is a significant predictor of whether a jury imposes a death sentence. However, less attention has been given to the effects of victim gender and to the combined effects of victim race and gender. With a growing scholarly interest in intersectionality, it is crucial to consider the interaction effect of victim race and gender on death sentencing outcomes. Data from the Capital Jury Project were used to examine whether the intersection of victim race and gender is associated with an increased odds of defendants receiving a death sentence and whether a “White female victim effect” exists. This study instead reveals a significant “Black male victim” effect. Black male victim cases are the least likely to be perceived by jurors as involving brutality, heinousness, and victim suffering. Results indicate that a defendant’s odds of receiving a death sentence in a White female victim case is 3.8 times greater and 3.6 times greater in a White male victim case than in a Black male victim case. Victim race rather than victim gender appears to be the factor most strongly influencing jury decision making in capital cases.