bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiscale Theories and Applications: From Microstructure Design to Macroscopic Assessment for Carbon Nanotubes Networks
Authors: Ji Jiachao , Jin Yulin , Hua Anping , Zhu Chunhua , Zhao Junhua
Published: January 2023

Carbon nanotube (CNT) networks enable CNTs to be used as building blocks for synthesizing novel advanced materials, thus taking full advantage of the superior properties of individual CNTs. Multiscale analyses have to be adopted to study the load transfer mechanisms of CNT networks from the atomic scale to the macroscopic scale due to the huge computational cost. Among them, fully resolved structural features include the graphitic honeycomb lattice (atomic), inter-tube stacking (nano) and assembly (meso) of CNTs. On an atomic scale, the elastic properties, ultimate stresses, and failure strains of individual CNTs with distinct chiralities and radii are obtained under various loading conditions by molecular mechanics. The dependence of the cohesive energies on spacing distances, crossing angles, size and edge effects between two CNTs is analyzed through continuum modeling in nanoscale. The mesoscale models, which neglect the atomic structures of individual CNTs but retain geometrical information about the shape of CNTs and their assembly into a network, have been developed to study the multi-level mechanism of material deformation and microstructural evolution in CNT networks under stretching, from elastic elongation, strengthening to damage and failure. This paper summarizes the multiscale theories mentioned above, which should provide insight into the optimal assembling of CNT network materials for elevated mechanical performance.
Side effects of Pfizer/BioNTech (BNT162b2) COVID-19 vaccine reported by the Birzeit University community
Authors: Abukhalil Abdallah Damin , Shatat Sireen Sultan , Abushehadeh Raya Riyad , Al-Shami Ni’meh , Naseef Hani A. , Rabba Abdullah
Published: January 2023

Background

The Pfizer BioNTech COVID-19 vaccine was the first to receive emergency authorization and approval from the FDA. Therefore, it is preferred by most recipients; however, many people are concerned about the vaccine’s side effects. At the time of the study, December 2021, Palestine lacked a national reporting system for monitoring adverse vaccine effects. Therefore, this study investigates the post-vaccine adverse events following the Pfizer/BioNTech COVID-19 Vaccine administration in Palestine and identifies the occurrence, extent, and severity among university staff, employees, and students at Birzeit University.

Method

A questionnaire-based retrospective cross-sectional study was conducted using a university website (Ritaj), social media platforms (e.g., Facebook and Telegram), and in-person interviews. The Chi-square, Fisher’s exact, and McNemar’s tests were used to investigate significant relationships. Data were analyzed using SPSS version 22.

Results

In total, 1137 participants completed the questionnaire, 33.2% were males, and the mean age was 21.163 years. All participants received at least one dose of the Pfizer-BioNTech COVID-19 vaccine. Approximately one-third of participants reported no adverse effects after receiving the first, second, or third doses (34%, 33.6%, and 32.5%, respectively). The most commonly reported adverse events were fever, chills, headache, fatigue, pain and swelling at the injection site, muscle pain, and joint pain. Allergic reactions were reported by 12.7% of the participants; furthermore, participants with a history of allergy or anaphylaxis before vaccination had a significantly higher tendency for post-vaccination allergic reactions. Eight participants reported rare side effects, including 7 (0.6%) cases of thrombocytopenia and one (0.1%) case of myocarditis. Males aged less than 20 years and smokers were significantly less likely to complain of adverse events. The number of reported side effects was significantly higher after the second vaccine dose than after the first dose. Finally, participants infected with COVID-19 before vaccination was significantly associated with side effects such as fever, chills, shortness of breath, and persistent cough.

Conclusion

In this study, the most common post- BNT162b2 Vaccination reported self-limiting side effects similar to those reported by Pfizer/BioNTech Company. However, higher rates of allergic reactions were reported in this sample. Rare side effects, such as thrombocytopenia and myocarditis, were reported by 8 participants. COVID vaccines have been developed at an accelerated pace, and vaccine safety is a top priority; therefore, standard monitoring through a national adverse event reporting system is necessary for safety assurance. Continuous monitoring and long-term studies are required to ensure vaccine safety.
Effect of intra-arterial nimodipine on iatrogenic vasospasms during endovascular stroke treatment – angiographic resolution and infarct growth in follow-up imaging
Authors: Jesser Jessica , Potreck Arne , Vollherbst Dominik , Seker Fatih , Chen Min , Schönenberger Silvia , Do Thuy D. , Bendszus Martin , Möhlenbruch Markus A. , Weyland Charlotte S.
Published: January 2023

Purpose

The treatment of vasospasms during endovascular stroke treatment (EST) with intra-arterial nimodipine (NM) is routinely performed. However, the efficacy of resolving iatrogenic vasospasms during the angiographic intervention and the infarct development in follow-up imaging after EST has not been studied yet.

Methods

Retrospective single-center analysis of patients receiving EST for anterior circulation vessel occlusion between 01/2015 and 12/2021. The primary endpoint was ASPECTS in follow-up imaging. Secondary endpoints were the clinical outcome (combined endpoint NIHSS 24 h after EST and difference between modified Rankin Scale (mRS) before stroke and at discharge (delta mRS)) and intracranial hemorrhage (ICH) in follow-up imaging. Patients with vasospasms receiving NM (NM+) or not (NM-) were compared in univariate analysis.

Results

Vasospasms occurred in 79/1283 patients (6.2%), who consecutively received intra-arterial NM during EST. The targeted vasospasm angiographically resolved in 84% (66/79) under NM therapy. ASPECTS was lower in follow-up imaging after vasospasms and NM-treatment (NM – 7 (6–9), NM + 6 (4.5-8), p = 0.013) and the clinical outcome was worse (NIHSS 24 h after EST was higher in patients treated with NM (median, IQR; NM+: 14, 5–21 vs. NM-: 9, 3–18; p = 0.004), delta-mRS was higher in the NM + group (median, IQR; NM+: 3, 1–4 vs. NM-: 2, 1–2; p = 0.011)). Any ICH (NM+: 27/79, 34.2% vs. NM-: 356/1204, 29.6%; p = 0.386) and symptomatic ICH (NM+: 2/79, 2.5% vs. NM-: 21/1204, 1.7%; p = 0.609) was equally distributed between groups.

Conclusion

Intra-arterial nimodipine during EST resolves iatrogenic vasospasms efficiently during EST without increasing intracranial hemorrhage rates. However, patients with vasospasms and NM treatment show higher infarct growth resulting in lower ASPECTS in follow-up imaging.
Treatment of bipolar clavicle injury with internal plating: a case series and literature review
Authors: Feng Dongxu , Liu Yong , Li Zijun , Huang Jie , Fan Mei , Kang Xiaomin , Zhang Jun
Published: January 2023

Background

Bipolar clavicle injury is a rare injury involving any combination of dislocation and/or fracture at both ends of the clavicle. Most reports of bipolar clavicle injury have been based on a single case, and treatment of this injury remains controversial. The present study was performed to evaluate the efficacy of surgical management with internal plating for bipolar clavicle injuries.

Methods

We performed internal plating to treat seven consecutive bipolar clavicle injuries with different injury patterns from May 2013 to June 2021. A clavicle hook plate was used for five sternoclavicular joint injuries (including a revision surgery) and three acromioclavicular joint dislocations, a T plate was used for one sternoclavicular joint injury, and an anatomic plate was used for one distal clavicle fracture. At follow-up, radiographs were assessed for bone alignment, joint congruity, fracture union or malunion, and implant failure or migration. Clinical evaluation included determination of the Disability of the Arm, Shoulder, and Hand (DASH) score; Constant–Murley score; visual analog scale (VAS) score; and complications.

Results

The patients were regularly followed up after the operation, and functional parameters were assessed over time. At a mean follow-up of 28.1 ± 22.0 months, each fracture had solid bone union, and each dislocation showed no sign of recurrent instability. The mean shoulder forward flexion was 159.3° ± 7.9°, and the mean DASH score was 8.8 ± 5.1. The mean Constant–Murley score was 88.9 ± 7.9, with six cases assessed as excellent and one case assessed as good. The mean VAS score was 1.0 ± 1.5, and the mean patient satisfaction score was 9.3 ± 0.8. No complications occurred, and each patient was able to resume their preinjury daily activity and was highly satisfied with their treatment.

Conclusions

In the present study, internal plating for bipolar clavicle injury allowed early mobilization and resulted in good joint function. We recommend fixation of the more severely affected side first because the other side may be passively reduced and acquire stability once the more severely affected side has been fixed. Internal fixation of the other end may therefore be unnecessary unless residual instability exists.
Exploring medical students’ perspectives of physician leadership
Authors: Vo Albert , Torti Jacqueline , Haddara Wael , Sultan Nabil
Published: January 2023

Background

Leadership has been recognized as an important competency in medicine. Nevertheless, leadership curricula for Canadian medical students lacks standardization and may not be informed by medical students’ perspectives of physician leadership. The purpose of this study was to elicit these perspectives on physician leadership.

Methods

The present study utilized semi-structured interviews to ascertain the views of medical student participants, including students in their first, second and third years of medical school, on physician leadership. Interview questions were based on ‘the 3-C model’ of physician leadership, which includes three aspects of leadership, namely character, competence and commitment. The interviews were audio-recorded, transcribed and then coded using thematic analysis.

Results

The medical students of this study provided rich examples of resident and staff physicians demonstrating effective and ineffective leadership. The participants identified the importance of character to effective physician leadership, but some participants also described a feeling of disconnect with the relevance of character at their stage of training. When discussing physician competence, medical students described the importance of both medical expertise and transferable skills. Lastly, the leadership aspect of commitment was identified as being relevant, but medical students cautioned against the potential for physician burnout. The medical student participants’ suggestions for improved leadership development included increased experiences with examples of physician leadership, opportunities to engage in leadership and participation in reflection exercises.

Conclusions

Overall, the study participants demonstrated an appreciation for three aspects of leadership; character, competence and commitment. Furthermore, they also provided recommendations for the future design of medical leadership curricula.
Weaving rapport: doctors’ strategies towards patients’ noncompliance
Authors: Tian Yinong , Zhang Jing , Che Haiyang , Su Yonggang
Published: January 2023

Background

A successful therapeutic rapport between doctors and patients is built on effective doctor-patient communication. Noncompliance of patients which challenges their communication has been described in the research, yet the rapport strategies are not well discussed.

Methods

This qualitative study investigates the rapport strategies when doctors face noncompliance in consultations and its pragmatic effects achieved through the doctors’ speeches. The 10-hour recordings come from the doctor-patient communication in the hospital setting. Thereafter, we analyze their conversation following the Spencer Oatey’s rapport management model.

Results

Compliments and joking in the illocutionary domain, storytelling in the discourse domain, the doctors’ participation in the participation domain and the choice of appropriate titles in the stylistic domain are identified and analyzed as the rapport-building strategies.

Conclusion

The present study has offered insights into physicians’ rapport-building strategies in the face of rapport-threatening behavior from patients. These strategies will help the doctors to deal with rapport-challenging behavior and boost overall patient wellness.
Costs and outcomes of colorectal cancer screening program in Isfahan, Iran
Authors: Rahimi Farimah , Rezayatmand Reza , Shojaeenejad Javad , Tabesh Elham , Ravankhah Zahra , Adibi Peyman
Published: January 2023

Background

Colorectal cancer is one of the most prevalent gastrointestinal cancers in Iran i.e., the fourth and the second prevalent cancer among Iranian males and females, respectively. A routine screening program is effective in the early detection of disease which can reduce the cancer burden both for individuals and society. In 2015, Iran’s Package of Essential Non- communicable Diseases program had been piloted in Shahreza city in Isfahan province. Colorectal cancer screening for the population aged 50–70 was a part of this program. So far, there was no study about the cost and outcomes of that program. Thus, this study aimed to analyze the costs and outcomes of colorectal cancer screening done from 2016 to 2019 in Shahreza.

Methods

This cost-outcome description study used the data of 19,392 individuals who were 50–70 years old experienced a fecal immunochemical test (FIT) and had an electronic health record. All direct costs including personnel, building space, equipment, training, etc. were extracted from the financial documents existing in the Isfahan province Health Center. The outcome was defined as positive FIT, detection of adenoma or malignancy as recorded in the E-integrated health system.

Results

The results of this study indicated that the direct costs of the colorectal cancer screening program during the years 2016–2019 were 7,368,707,574 Rials (321,029 PPP$) in Shahreza, Isfahan province. These costs resulted in identifying 821 people with a positive FIT test, of those 367 individuals were undergone colonoscopy. Of whom 8 cases of colorectal cancer, and 151 cases with polyps were diagnosed.

Conclusion

This study showed that by paying a small amount of 320 thousand international dollars we could prevent 151 cases of polyps to be progressed to colorectal cancer,resulting in a significant reduction in colorectal cancer incidence.
Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects
Authors: Jin Minghui , Liu Bo , Zheng Weigang , Liu Conghui , Liu Zhenxing , He Yuan , Li Xiaokang , Wu Chao , Wang Ping , Liu Kaiyu , Wu Shigang , Liu Hangwei , Chakrabarty Swapan , Yuan Haibin , Wilson Kenneth , Wu Kongming , Fan Wei , Xiao Yutao
Published: January 2023

Background

The black cutworm, Agrotis ipsilon, is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute to its widening distribution and increasing difficulty of control. However, knowledge about these traits is still limited.

Results

We generated a high-quality chromosome-level assembly of A. ipsilon using PacBio and Hi-C technology with a contig N50 length of ~ 6.7 Mb. Comparative genomic and transcriptomic analyses showed that detoxification-associated gene families were highly expanded and induced after insects fed on specific host plants. Knockout of genes that encoded two induced ABC transporters using CRISPR/Cas9 significantly reduced larval growth rate, consistent with their contribution to host adaptation. A comparative transcriptomic analysis between tethered-flight moths and migrating moths showed expression changes in the circadian rhythm gene AiCry2 involved in sensing photoperiod variations and may receipt magnetic fields accompanied by MagR and in genes that regulate the juvenile hormone pathway and energy metabolism, all involved in migration processes.

Conclusions

This study provides valuable genomic resources for elucidating the mechanisms involved in moth migration and developing innovative control strategies.
Effects of mesenchymal stem cell-derived nanovesicles in experimental allergic airway inflammation
Authors: Bandeira Elga , Jang Su Chul , Lässer Cecilia , Johansson Kristina , Rådinger Madeleine , Park Kyong-Su
Published: January 2023

Background

Allergic asthma is associated with airflow obstruction and hyper-responsiveness that arises from airway inflammation and remodeling. Cell therapy with mesenchymal stem cells (MSC) has been shown to attenuate inflammation in asthma models, and similar effects have recently been observed using extracellular vesicles (EV) obtained from these cells. Biologically functional vesicles can also be artificially generated from MSC by extruding cells through membranes to produce EV-mimetic nanovesicles (NV). In this study, we aimed to determine the effects of different MSC-derived vesicles in a murine model of allergic airway inflammation.

Methods

EV were obtained through sequential centrifugation of serum-free media conditioned by human bone marrow MSC for 24 h. NV were produced through serial extrusion of the whole cells through filters. Both types of vesicles underwent density gradient purification and were quantified through nanoparticle tracking analysis. C57BL/6 mice were sensitized to ovalbumin (OVA, 8 µg), and then randomly divided into the OVA group (intranasally exposed to 100 µg OVA for 5 days) and control group (exposed to PBS). The mice were then further divided into groups that received 2 × 109 EV or NV (intranasally or intraperitoneally) or PBS immediately following the first OVA exposure.

Results

Administration of EV and NV reduced cellularity and eosinophilia in bronchoalveolar lavage (BAL) fluid in OVA-sensitized and OVA-exposed mice. In addition, NV treatment resulted in decreased numbers of inflammatory cells within the lung tissue, and this was associated with lower levels of Eotaxin-2 in both BAL fluid and lung tissue. Furthermore, both intranasal and systemic administration of NV were effective in reducing inflammatory cells; however, systemic delivery resulted in a greater reduction of eosinophilia in the lung tissue.

Conclusions

Taken together, our results indicate that MSC-derived NV significantly reduce OVA-induced allergic airway inflammation to a level comparable to EV. Thus, cell-derived NV may be a novel EV-mimetic therapeutic candidate for treating allergic diseases such as asthma.
Malaria among children under 10 years in 4 endemic health areas in Kisantu Health Zone: epidemiology and transmission
Authors: Ilombe Gillon , Matangila Junior Rika , Lulebo Aimee , Mutombo Paulin , Linsuke Sylvie , Maketa Vivi , Mabanzila Baby , Wat’senga Francis , Van Bortel Wim , Fiacre Agossa , Irish Seth R. , Lutumba Pascal , Van Geertruyden Jean-Pierre
Published: January 2023

Background

The Democratic Republic of the Congo (DRC) is the second most malaria-affected country in the world with 21,608,681 cases reported in 2019. The Kongo Central (KC) Province has a malaria annual incidence of 163 cases/per 1000 inhabitants which are close to the national average of 153.4/1000. However, the malaria prevalence varies both between and within health zones in this province. The main objective of this study was to describe the epidemiology and transmission of malaria among children aged 0 to 10 years in the 4 highest endemic health areas in Kisantu Health Zone (HZ) of KC in DRC.

Methods

A community-based cross-sectional study was conducted from October to November 2017 using multi-stage sampling. A total of 30 villages in 4 health areas in Kisantu HZ were randomly selected. The prevalence of malaria was measured using a thick blood smear (TBS) and known predictors and associated outcomes were assessed. Data are described and association determinants of malaria infection were analysed.

Results

A total of 1790 children between 0 and 10 years were included in 30 villages in 4 health areas of Kisantu HZ. The overall prevalence in the study area according to the TBS was 14.8% (95% CI: 13.8–16.6; range: 0–53). The mean sporozoite rate in the study area was 4.3% (95% CI: 2.6–6.6). The determination of kdr-west resistance alleles showed the presence of both L1014S and L1014F with 14.6% heterozygous L1014S/L1014F, 84.4% homozygous 1014F, and 1% homozygous 1014S. The risk factors associated with malaria infection were ground or wooden floors aOR: 15.8 (95% CI: 8.6–29.2), a moderate or severe underweight: 1.5 (1.1–2.3) and to be overweight: 1.9 (95% CI: 1.3–2.7).

Conclusion

Malaria prevalence differed between villages and health areas within the same health zone. The control strategy activities must be oriented by the variety in the prevalence and transmission of malaria in different areas. The policy against malaria regarding long-lasting insecticidal nets should be based on the evidence of metabolic resistance.
Neighborhood infrastructure-related risk factors and non-communicable diseases: a systematic meta-review
Authors: Zhang Yuyang , Liu Ningrui , Li Yan , Long Ying , Baumgartner Jill , Adamkiewicz Gary , Bhalla Kavi , Rodriguez Judith , Gemmell Emily
Published: January 2023

Background

With rapid urbanization, the urban environment, especially the neighborhood environment, has received increasing global attention. However, a comprehensive overview of the association between neighborhood risk factors and human health remains unclear due to the large number of neighborhood risk factor–human health outcome pairs.

Method

On the basis of a whole year of panel discussions, we first obtained a list of 5 neighborhood domains, containing 33 uniformly defined neighborhood risk factors. We only focused on neighborhood infrastructure-related risk factors with the potential for spatial interventions through urban design tools. Subsequently, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic meta-review of 17 infrastructure-related risk factors of the 33 neighborhood risk factors (e.g., green and blue spaces, proximity to major roads, and proximity to landfills) was conducted using four databases, Web of Science, PubMed, OVID, and Cochrane Library, from January 2000 to May 2021, and corresponding evidence for non-communicable diseases (NCDs) was synthesized. The review quality was assessed according to the A MeaSurement Tool to Assess Systematic Reviews (AMSTAR) standard.

Results

Thirty-three moderate-and high-quality reviews were included in the analysis. Thirteen major NCD outcomes were found to be associated with neighborhood infrastructure-related risk factors. Green and blue spaces or walkability had protective effects on human health. In contrast, proximity to major roads, industry, and landfills posed serious threats to human health. Inconsistent results were obtained for four neighborhood risk factors: facilities for physical and leisure activities, accessibility to infrastructure providing unhealthy food, proximity to industry, and proximity to major roads.

Conclusions

This meta-review presents a comprehensive overview of the effects of neighborhood infrastructure-related risk factors on NCDs. Findings on the risk factors with strong evidence can help improve healthy city guidelines and promote urban sustainability. In addition, the unknown or uncertain association between many neighborhood risk factors and certain types of NCDs requires further research.
Tools for assessing health research partnership outcomes and impacts: a systematic review
Authors: Mrklas K. J. , Boyd J. M. , Shergill S. , Merali S. , Khan M. , Nowell L. , Goertzen A. , Pfadenhauer L. M. , Paul K. , Sibley K. M. , Swain L. , Vis-Dunbar M. , Hill M. D. , Raffin-Bouchal S. , Tonelli M. , Graham I. D.
Published: January 2023

Objective

To identify and assess the globally available valid, reliable and acceptable tools for assessing health research partnership outcomes and impacts.

Methods

We searched Ovid MEDLINE, Embase, CINAHL Plus and PsycINFO from origin to 2 June 2021, without limits, using an a priori strategy and registered protocol. We screened citations independently and in duplicate, resolving discrepancies by consensus and retaining studies involving health research partnerships, the development, use and/or assessment of tools to evaluate partnership outcomes and impacts, and reporting empirical psychometric evidence. Study, tool, psychometric and pragmatic characteristics were abstracted using a hybrid approach, then synthesized using descriptive statistics and thematic analysis. Study quality was assessed using the quality of survey studies in psychology (Q-SSP) checklist.

Results

From 56 123 total citations, we screened 36 027 citations, assessed 2784 full-text papers, abstracted data from 48 studies and one companion report, and identified 58 tools. Most tools comprised surveys, questionnaires and scales. Studies used cross-sectional or mixed-method/embedded survey designs and employed quantitative and mixed methods. Both studies and tools were conceptually well grounded, focusing mainly on outcomes, then process, and less frequently on impact measurement. Multiple forms of empirical validity and reliability evidence was present for most tools; however, psychometric characteristics were inconsistently assessed and reported. We identified a subset of studies (22) and accompanying tools distinguished by their empirical psychometric, pragmatic and study quality characteristics. While our review demonstrated psychometric and pragmatic improvements over previous reviews, challenges related to health research partnership assessment and the nascency of partnership science persist.

Conclusion

This systematic review identified multiple tools demonstrating empirical psychometric evidence, pragmatic strength and moderate study quality. Increased attention to psychometric and pragmatic requirements in tool development, testing and reporting is key to advancing health research partnership assessment and partnership science.

PROSPEROCRD42021137932
Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection
Authors: Taban Qamar , Ahmad Syed Mudasir , Mumtaz Peerzada Tajamul , Bhat Basharat , Haq Ehtishamul , Magray Suhail , Saleem Sahar , Shabir Nadeem , Muhee Amatul , Kashoo Zahid Amin , Zargar Mahrukh Hameed , Malik Abrar A. , Ganai Nazir A. , Shah Riaz A.
Published: January 2023

SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host–pathogen interactions by acting in co-ordination with Toll-like receptors.Query Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was significant in induced GMECs at 6 h. Endoribonuclease-esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections.
On-farm diversity, use pattern, and conservation of enset (Ensete ventricosum) genetic resources in southern Ethiopia
Authors: Dilebo Tesfaye , Feyissa Tileye , Asfaw Zemede , Zewdu Ashagire
Published: January 2023

Background

Enset is an important source of food and is consumed by about 25 million people as a staple or co-staple food crop mainly in southern parts of Ethiopia. Large numbers of enset landraces exist in different administrative zones of Ethiopia with a wide range of altitudes and agroclimatic zones. However, limited information is available on the diversity, distribution, and utilization pattern corresponding to the diverse ethnolinguistic as well as sociocultural communities of the country. Hence, this study was devised to explore and document the richness of farmers’ tradition and practice on the diversity and distribution of enset landraces on the farm level and selection pattern for different purposes regarding the production, utilization, and conservation of enset genetic resources.

Methods

The study was conducted in four major enset-growing administrative zones of Ethiopia, namely Hadiya, Kembata-Tembaro, Gurage, and Silte. A total of 240 farm households were surveyed using individual interviews, 18 key informant interviews, 36 focus group discussions with 5 participants, and direct on-farm field observations for data collection. Considering that enset has a rich cultural background and indigenous knowledge, ethnobotanical research approach was applied to data collection and analysis. The Shannon–Weaver, Simpson, Pielou, and Jaccard’s similarity indices were used to evaluate the diversity and similarity of the landraces as well as using descriptive statistics in SPSS Ver. 24. Preference in direct matrix ranking was also used to compute and rank the enset landraces most preferred by the people in the context of specific use value in the study area.

Results

A total of 282 farmer-named enset landraces have been identified, with a range from 2 to 32 on individual homegardens. The largest number of landraces was found in the Hadiya Zone (86), while the lowest was scored in the Silte Zone (57). The Shannon diversity index (H') ranged from 3.73 (Silte) to 3.96 (Hadiya). Similarly, landraces revealed a very narrow range of variances in Simpson’s 1-D diversity index, and it ranged from 0.963 (Silte) to 0.978 (Hadiya). Likewise, the similarity index ranged from 0.24 to 0.73 sharing 16–47 landraces in common. Of the 282 landraces, 210 (74.5%) were recorded in more than one zones, whereas 72 (25.5%) had narrow distribution being restricted to a single zone.

Conclusions

Farmers have established long-term practices and experiences in cultivation, utilization, and conservation of a diverse group of enset landraces to fill their domestic and market purposes in each zone. The variation is likely to be related to agroclimatic differences, ethnicity factors, food cultures, and historical backgrounds. Therefore, to facilitate on-farm conservation as well as sustainable utilization of the enset genetic resources, farmers need to be supported by different stakeholders for all their worth and also in crop improvement programs.
Cavernous mediastinal hemangioma presenting with persistent cough: a rare case report and review of literature
Authors: Mardani Parviz , Kamran Hooman , Geramizadeh Bita , Darabi Mohammad Hassan , Najafi Masoud , Amirian Armin , Shahriarirad Reza
Published: January 2023

Background

Cavernous hemangioma is a rare benign tumor which can sometimes mimic the clinical presentation and radiological findings of malignant tumors. Here we present a rare presentation of cavernous hemangioma in the mediastinum (CHM), along with a literature review among the main databases.

Case presentation

We present a 48-year-old male who had suffered from persistent cough as the sole symptom of an anterior CHM. Computed tomography scan demonstrated a 12.5 × 10.8 cm mass in the anterior mediastinum. The mass was surgically resected, and histopathological evaluation established the diagnosis of CHM. The patient was discharged in good condition, in which during his four-month follow-up period, no recurrence of the tumor has been observed.

Conclusion

Although cavernous hemangioma rarely present in the mediastinum, it should be considered in the differential diagnosis of mediastinal tumors. However, our review of literature demonstrated a female dominance and average age of 40 years, with a 52% mortality rate based on previous reports.
Diagnostic performance of Glial Fibrillary Acidic Protein and Prehospital Stroke Scale for identification of stroke and stroke subtypes in an unselected patient cohort with symptom onset < 4.5 h
Authors: Jæger Henriette S. , Tranberg Ditte , Larsen Karianne , Valentin Jan B. , Blauenfeldt Rolf A. , Luger Sebastian , Bache Kristi G. , Gude Martin F.
Published: January 2023

Introduction

Rapid identification and treatment of stroke is crucial for the outcome of the patient. We aimed to determine the performance of glial fibrillary acidic protein (GFAP) independently and in combination with the Prehospital Stroke Score (PreSS) for identification and differentiation of acute stroke within 4.5 h after symptom onset.

Patients and methods

Clinical data and serum samples were collected from the Treat-Norwegian Acute Stroke Prehospital Project (Treat-NASPP). Patients with suspected stroke and symptoms lasting ≤ 4.5 h had blood samples collected and were evaluated with the National Institutes of Health Stroke Scale prospectively. In this sub study, NIHSS was retrospectively translated into PreSS and GFAP was measured using the sensitive single molecule array (SIMOA).

Results

A total of 299 patients with suspected stroke were recruited from Treat-NASPP and included in this study (44% acute ischemic stroke (AIS), 10% intracranial hemorrhage (ICrH), 7% transient ischemic attack (TIA), and 38% stroke mimics). ICrH was identified with a cross-fold validated area under the receiver-operating characteristic curve (AUC) of 0.73 (95% CI 0.62–0.84). A decision tree with PreSS and GFAP combined, first identified patients with a low probability of stroke. Subsequently, GFAP detected patients with ICrH with a 25.0% sensitivity (95% CI 11.5–43.4) and 100.0% specificity (95% CI 98.6–100.0). Lastly, patients with large-vessel occlusion (LVO) were detected with a 55.6% sensitivity (95% CI 35.3–74.5) and 82.4% specificity (95% CI 77.3–86.7).

Conclusion

In unselected patients with suspected stroke, GFAP alone identified ICrH. Combined in a decision tree, GFAP and PreSS identified subgroups with high proportions of stroke mimics, ICrH, LVO, and AIS (non-LVO strokes).
Enzymatic debranching is a key determinant of the xylan-degrading activity of family AA9 lytic polysaccharide monooxygenases
Authors: Tõlgo Monika , Hegnar Olav A. , Larsbrink Johan , Vilaplana Francisco , Eijsink Vincent G. H. , Olsson Lisbeth
Published: January 2023

Background

Previous studies have revealed that some Auxiliary Activity family 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) oxidize and degrade certain types of xylans when incubated with mixtures of xylan and cellulose. Here, we demonstrate that the xylanolytic activities of two xylan-active LPMOs, TtLPMO9E and TtLPMO9G from Thermothielavioides terrestris, strongly depend on the presence of xylan substitutions.

Results

Using mixtures of phosphoric acid-swollen cellulose (PASC) and wheat arabinoxylan (WAX), we show that removal of arabinosyl substitutions with a GH62 arabinofuranosidase resulted in better adsorption of xylan to cellulose, and enabled LPMO-catalyzed cleavage of this xylan. Furthermore, experiments with mixtures of PASC and arabinoglucuronoxylan from spruce showed that debranching of xylan with the GH62 arabinofuranosidase and a GH115 glucuronidase promoted LPMO activity. Analyses of mixtures with PASC and (non-arabinosylated) beechwood glucuronoxylan showed that GH115 action promoted LPMO activity also on this xylan. Remarkably, when WAX was incubated with Avicel instead of PASC in the presence of the GH62, both xylan and cellulose degradation by the LPMO9 were impaired, showing that the formation of cellulose–xylan complexes and their susceptibility to LPMO action also depend on the properties of the cellulose. These debranching effects not only relate to modulation of the cellulose–xylan interaction, which influences the conformation and rigidity of the xylan, but likely also affect the LPMO–xylan interaction, because debranching changes the architecture of the xylan surface.

Conclusions

Our results shed new light on xylanolytic LPMO9 activity and on the functional interplay and possible synergies between the members of complex lignocellulolytic enzyme cocktails. These findings will be relevant for the development of future lignocellulolytic cocktails and biomaterials.
Deep learning enables automated MRI-based estimation of uterine volume also in patients with uterine fibroids undergoing high-intensity focused ultrasound therapy
Authors: Theis Maike , Tonguc Tolga , Savchenko Oleksandr , Nowak Sebastian , Block Wolfgang , Recker Florian , Essler Markus , Mustea Alexander , Attenberger Ulrike , Marinova Milka , Sprinkart Alois M.
Published: January 2023

Background

High-intensity focused ultrasound (HIFU) is used for the treatment of symptomatic leiomyomas. We aim to automate uterine volumetry for tracking changes after therapy with a 3D deep learning approach.

Methods

A 3D nnU-Net model in the default setting and in a modified version including convolutional block attention modules (CBAMs) was developed on 3D T2-weighted MRI scans. Uterine segmentation was performed in 44 patients with routine pelvic MRI (standard group) and 56 patients with uterine fibroids undergoing ultrasound-guided HIFU therapy (HIFU group). Here, preHIFU scans (n = 56), postHIFU imaging maximum one day after HIFU (n = 54), and the last available follow-up examination (n = 53, days after HIFU: 420 ± 377) were included. The training was performed on 80% of the data with fivefold cross-validation. The remaining data were used as a hold-out test set. Ground truth was generated by a board-certified radiologist and a radiology resident. For the assessment of inter-reader agreement, all preHIFU examinations were segmented independently by both.

Results

High segmentation performance was already observed for the default 3D nnU-Net (mean Dice score = 0.95 ± 0.05) on the validation sets. Since the CBAM nnU-Net showed no significant benefit, the less complex default model was applied to the hold-out test set, which resulted in accurate uterus segmentation (Dice scores: standard group 0.92 ± 0.07; HIFU group 0.96 ± 0.02), which was comparable to the agreement between the two readers.

Conclusions

This study presents a method for automatic uterus segmentation which allows a fast and consistent assessment of uterine volume. Therefore, this method could be used in the clinical setting for objective assessment of therapeutic response to HIFU therapy.
Neural stem/progenitor cell therapy for Alzheimer disease in preclinical rodent models: a systematic review and meta-analysis
Authors: Zhou Zijing , Shi Ben , Xu Yaxing , Zhang Jinyu , liu Xin , Zhou Xinghong , Feng Baofeng , Ma Jun , Cui Huixian
Published: January 2023

Background

Alzheimer’s disease (AD) is a common progressive neurodegenerative disease characterized by memory impairments, and there is no effective therapy. Neural stem/progenitor cell (NSPC) has emerged as potential novel therapy for AD, and we aim to explore whether neural stem/progenitor cell therapy was effective for rodent models of AD.

Methods

We searched PubMed, Embase, Cochrane Library and Web of Science up to December 6, 2022. The outcomes included cognitive function, pathological features and BDNF. The GetData Graph Digitizer software (version 2.26) was applied to extract numerical values, and RevMan 5.3 and Stata 16 were used to analyze data. The SYRCLE risk of bias tool was used to assess study quality.

Results

We evaluated 22 mice studies and 8 rat studies. Compared to control groups, cognitive function of NSPC groups of both mice studies (SMD =  − 1.96, 95% CI  − 2.47 to  − 1.45, I2 = 75%, P < 0.00001) and rat studies (SMD =  − 1.35, 95% CI − 2.11 to  − 0.59, I2 = 77%, P = 0.0005) was apparently improved. In mice studies, NSPC group has lower Aβ deposition (SMD =  − 0.96, 95% CI  − 1.40 to  − 0.52, P < 0.0001) and p-tau level (SMD =  − 4.94, 95% CI  − 7.29 to  − 2.95, P < 0.0001), higher synaptic density (SMD = 2.02, 95% CI 0.50–3.55, P = 0.009) and BDNF (SMD = 1.69, 95% CI 0.61–2.77, P = 0.002). Combined with nanoformulation (SMD =  − 1.29, 95% CI  − 2.26 to  − 0.32, I2 = 65%, P = 0.009) and genetically modified (SMD =  − 1.29, 95% CI  − 1.92 to  − 0.66, I2 = 60%, P < 0.0001) could improve the effect of NSPC. In addition, both xenogeneic and allogeneic transplant of NSPC could reverse the cognitive impairment of AD animal models.

Conclusions

Our results suggested that NSPC therapy could improve the cognitive function and slow down the progression of AD. Due to the limitations of models, more animal trials and clinical trials are needed.
Laminin-binding protein of Streptococcus suis serotype 2 influences zinc acquisition and cytokine responses
Authors: Payen Servane , Rrodriguez Jesús Aranda , Segura Mariela , Gottschalk Marcelo
Published: January 2023

Streptococcus suis serotype 2 is an important bacterial pathogen of swine, responsible for substantial economic losses to the swine industry worldwide. The knowledge on the pathogenesis of the infection caused by S. suis is still poorly known. It has been previously described that S. suis possesses at least one lipoprotein with double laminin and zinc (Zn)-binding properties, which was described in the literature as either laminin-binding protein (Lmb, as in the current study), lipoprotein 103, CDS 0330 or AdcAII. In the present study, the role of the Lmb in the pathogenesis of the infection caused by S. suis serotype 2 was dissected. Using isogenic mutants, results showed that Lmb does not play an important role in the laminin-binding activity of S. suis, even when clearly exposed at the bacterial surface. In addition, the presence of this lipoprotein does not influence bacterial adhesion to and invasion of porcine respiratory epithelial and brain endothelial cells and it does not increase the susceptibility of S. suis to phagocytosis. On the other hand, the Lmb was shown to play an important role as cytokine activator when tested in vitro with dendritic cells. Finally, this lipoprotein plays a critical role in Zn acquisition from the host environment allowing bacteria to grow in vivo. The significant lower virulence of the Lmb defective mutant may be related to a combination of a lower bacterial survival due to the incapacity to acquire Zn from their surrounding milieu and a reduced cytokine activation.
Development of a 32-gene signature using machine learning for accurate prediction of inflammatory bowel disease
Authors: Yu Shicheng , Zhang Mengxian , Ye Zhaofeng , Wang Yalong , Wang Xu , Chen Ye-Guang
Published: January 2023

Inflammatory bowel disease (IBD) is a chronic inflammatory condition caused by multiple genetic and environmental factors. Numerous genes are implicated in the etiology of IBD, but the diagnosis of IBD is challenging. Here, XGBoost, a machine learning prediction model, has been used to distinguish IBD from healthy cases following elaborative feature selection. Using combined unsupervised clustering analysis and the XGBoost feature selection method, we successfully identified a 32-gene signature that can predict IBD occurrence in new cohorts with 0.8651 accuracy. The signature shows enrichment in neutrophil extracellular trap formation and cytokine signaling in the immune system. The probability threshold of the XGBoost-based classification model can be adjusted to fit personalized lifestyle and health status. Therefore, this study reveals potential IBD-related biomarkers that facilitate an effective personalized diagnosis of IBD.
On new Milne-type inequalities and applications
Authors: Bosch Paul , Rodríguez José M. , Sigarreta José M.
Published: January 2023

Inequalities play a major role in pure and applied mathematics. In particular, the inequality plays an important role in the study of Rosseland’s integral for the stellar absorption. In this paper we obtain new Milne-type inequalities, and we apply them to the generalized Riemann–Liouville-type integral operators, which include most of the known Riemann–Liouville integral operators.
Lightweight similarity checking for English literatures in mobile edge computing
Authors: Liu Xiaomei , Gao Ailing , Chen Chengxiang , Moghimi Mohammad Mahdi
Published: January 2023

With the advent of information age, mobile devices have become one of the major convenient equipment that aids people’s daily office activities such as academic research, one of whose major tasks is to check the repetition rate or similarity among different English literatures. Traditional literature similarity checking solutions in cloud paradigm often call for intensive computational cost and long waiting time. To tackle this issue, in this paper, we modify the traditional literature similarity checking solution in cloud paradigm to make it suitable for the light-weight mobile edge environment. Furthermore, we put forward a lightweight similarity checking approach $$\mathrm {SC_{MEC}}$$

SC
MEC

for English literatures in mobile edge computing environment. To validate the advantages of $$\mathrm {SC_{MEC}}$$

SC
MEC

, we have designed massive experiments on a dataset. The reported experimental results show that $$\mathrm {SC_{MEC}}$$

SC
MEC

can deliver a satisfactory similarity checking result of literatures compared to other existing approaches.
Correlates of resilience among university students in Oman: a cross-sectional study
Authors: Al Omari Omar , Al Yahyaei Asma , Wynaden Dianne , Damra Jalal , Aljezawi Maen , Al Qaderi Mohammad , Al Ruqaishi Huda , Abu Shahrour Loai , ALBashtawy Mohammed
Published: January 2023

Background

Resilience has a paramount role in dealing with different life stressors and has a great impact on mental health.

Aim

To assess the level of resilience among university students and explore the relation between resilience and a number of variables including psychological well-being, self-esteem and physical health.

Methods

A cross-sectional design was utilized. Data was collected from 676 university students. Students were required to complete a demographic sheet, Brief Resilience Scale, World Health Organization Well-Being Index, Rosenberg Self-Esteem Scale and a physical health survey.

Results

Overall, less than half of the participants have reported low levels of resilience (45.3%; n = 306). Regular sleep, perceived stress, WHO well-being index, self-esteem, and having a cumulative GPA of more than two, were factors that significantly predicted positive resilience in multivariate analysis.

Conclusion

Resilience is a necessary skill among university students that requires more academic attention. Factors predicting positive resilience should be considered when implementing mental health promotion programs.