bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality Evaluation of Refrigerated Salted Butter Made with Goat and Cow Milk
Authors: Pradana Mahendra Wahyu Eka , Murti Tridjoko Wisnu
Published: February 2024

Butter is a dairy product that is susceptible to oxidation, which causes a rancid taste and a short shelf life. The quality of the butter depends on the quality of milk. The aim of the study is to determine the quality of butter made from goat and cow reared by small-scale farmers in Yogyakarta with the traditional manufacturing process. The milk of Saanen-Peranakan Ettawa (Sapera)  goats and Fresian Holstein were taken from farmers in Sleman district, Yogyakarta, Indonesia. Butters were sampled after 0, 15, 30 days storage. This study used the Nested Design method , namely the nested quality of butter in the type of milk. The result showed that butter made from cow milk produced a higher fat content compared to goat milk butter (P<0.05), namely 85.29 ± 0.04% and 80.10 ± 0.27%. The acid value increased while pH value was decreased during storage (P<0.05). There was no increase in peroxide value in cow butter but there was an increase in goat butter on day 0 to day 15 (P<0.05). It can be concluded that the goat and cow milk reared by small-scale farmers in Yogyakarta have good quality so it produced good quality butter which is still good for consumption until 30 days of storage in the refrigerator.
Fatty Acid Profiling of Bali and Wagyu Cattle using Principal Component Analysis
Authors: Dairoh Dairoh , Sutikno Sutikno , Ishak Andi Baso Lompengeng , Priyanto Rudy , Sumantri Cece , Ulum Mokhamad Fakhrul , Jakaria Jakaria
Published: February 2024

This study aimed to compare the fatty acid profiles of Bali cattle and Wagyu cattle. A total of 50 beef was used in this study, consisting of 44 Bali cattle from Kupang, NTT, and 6 samples of Wagyu cattle from supermarkets. The fatty acid profiles identified are saturated and unsaturated (MUFA and PUFA). The fatty acid analysis used is the Gas Chromatography (GC) method. Descriptive analysis was used to examine data on the fatty acid profile, and T-test analyzed fatty acid composition differences between Bali and Wagyu beef. Differences in fatty acid compositions have been reported based on breeds. The Bali beef had significantly (p<0.05) higher saturated fatty acid than Wagyu. Several fatty acids of meat from Bali cattle were significantly different (p<0.05) from Wagyu, except for myristoleic (C14:0) and palmitoleic acids (C16:0) did not show significant differences (p>0.05). The principal component analysis (PCA) results showed that the first principal component was UFA, MUFA: SFA ratio, oleic acid, omega-9, MUFA, and palmitic acid. In contrast, the second principal component was myristoleic acid, linolenic acid, omega-3, PUFA: SFA ratio, PUFA, omega-6, linoleic acid, stearic acid, SFA, and palmitoleic acid. The study's findings revealed that Bali beef had a much more saturated fatty acid composition of Bali beef was higher than Wagyu beef. This result suggests that Wagyu cattle have a more favorable fatty acid profile, which benefits health.
The Effects of Protected Feed Supplements on Estrus Response, Milk Production and Composition of Sapera Dairy Goats
Authors: Pramono Dr. Ahmad , Muhammad Muhammad , Riyadi Fairuz Izzudin , Cahyadi Muhammad
Published: February 2024

Soybean groats and earthworms (Lumbricus rubellus) are natural sources of protein, which are easy to obtain. Therefore, this research aims to evaluate the effects of protected soybean groats (PSG) and earthworm meal (PEM) in the diet on estrus response, milk production and composition of Saanen Peranakan Etawa (Sapera) dairy goats. These feed supplements were further protected by 1% formaldehyde. A completely randomized design with three treatments consisting of basal diet (P0), 75% basal diet + 25% PSG (P1), and 74.96% basal diet +24.99% PSG and 0.05% PEM (P2) was used in this research. Also, a total of 21 lactating Sapera goats were utilized and equally grouped into these treatments. Milk productions and composition were recorded and checked using a Lactoscan milk analyzer. Moreover, estrus synchronization was applied using the PGF2α and GnRH hormone using the intramuscular (IM) injection method and observing estrus. The data were analyzed using one-way analysis of variance while the differentiation of treatments was conducted with Duncan's multiple range test (DMRT). Electrical current resistance, vaginal temperature, vulva color, milk production, fat, total solids, and protein contents were observed to be significantly affected by the supplementation of PSG and PEM (P<0.05). In conclusion, supplementation of PSG and PEM in the diet could improve estrus response, milk production and composition of Sapera goats.
Analisis Tahapan Konseling Pastoral dengan Pendekatan Mindfulness terhadap Remaja Dengan Gangguan Kecemasan
Authors: Norpi Norpi , Sri Silvia Yelinda
Published: February 2024

Abstract
Anxiety problems are common and can be felt by every individual. However, when a person experiences anxiety, it can have a negative impact on their survival, both for themselves and for their environment. This study aims to analyze the stages of pastoral counseling with a mindfulness approach to adolescents who often experience anxiety. This research uses the method. Action research (action research). based on the results of the study that the mindfulness approach with deep breathing techniques and visual imagery when applied in the pastoral counseling process guided by the stage of pastoral konseling is effective in minimizing or reducing feelings of anxiety and even fear, giving a sense of relaxation, calm, comfort so that the counselee is better able to control his mind and himself. As a result, adolescents who previously often experienced anxiety when they saw family situations/conditions were in conflict could be minimized by regulating their breathing. This is very important because the anxiety that often strikes can hinder the relationship/relation with the family to be not good.
Analisis Validitas Instrument Self-Efficacy Belajar Siswa Pada Sekolah Terbuka Atlet Dengan Menggunakan Model Rasch
Authors: Haifa Munajjah Siti Najmi , Supriatna Mamat , Suryana Dodi
Published: February 2024

Self-Efficacy is an individual's belief in his own ability and competence to carry out his duties or activities which is demonstrated by the individual's way of acting and overcoming existing obstacles to succeed in achieving various goals. This research aims to test the validity and reliability of students' learning self-efficacy instruments using the Rasch model. The subjects of this research were students who took part in the athlete's open school program. The method used is a cross-sectional survey with a quantitative approach. The number of participants used was 69 students at one of the State High Schools in Bandung City, West Java, Indonesia with an age range of 15-17 years. Data analysis using the Rasch model in the WinStep 3.73 application. The results of the analysis show that the respondent's interaction with the items is in the good category. The respondent's reliability value, which shows the respondent's consistency in filling out the instrument, is included in the sufficient category. The reliability of the instrument shows that it is in the very good category. The unidimensional value is included in the sufficient category. Apart from that, there are three items that are not appropriate. It is hoped that with the results of this research, instruments for measuring students' learning self-efficacy can be evaluated and developed so that an accurate instrument can be obtained in measuring students' learning self-efficacy
Meningkatkan Strategi Belajar Peserta Didik Melalui Layanan Bimbingan Kelompok Menggunakan Metode Cooperatif Learning
Authors: Saputri Sandra Sari , Maftuhah Siti , Masril Masril
Published: February 2024

Weak student learning strategies lead to low learning outcomes so group guidance is carried out using the discussion method, but the activity objectives have not been achieved. Therefore used a group guidance approach with cooperative learning methods to improve student learning strategies. The research subjects were students of class VII UPT SMPN 2 Sitiung. This study uses the Guidance and Counseling Action Research method. Data was tested using the Paired Samples Test (T test). The results of the group guidance approach with the Cooperative Learning method can significantly improve student learning strategies.
Keefektifan Teknik Cinematherapy untuk Mengurangi Kebiasaan Mengonsumsi Minuman Keras Mahasiswa Maumere Universitas PGRI Kanjuruhan Malang
Authors: Wulan Sari Eva Kartika , Kowan Lydia Marlina
Published: February 2024

Consuming too much alcohol will affect one's attitudes and actions. The factors that can trigger this habit can be in the form of an environment that has a habit of consuming liquor, joining friends, experimenting, and so on. Apart from that, consuming liquor is also usually used by some people as an escape from the problems that are being experienced, where the intoxicating effect of alcohol in liquor can provide a momentary sense of calm for those who consume it. This study aims to reduce the level of drinking habits in Maumere students with cienematherapy techniques with pre-experiments with a one-group pretest and post-test design. The research subjects were eight students who had high criteria for the habit of consuming liquor. Giving treatment using group counseling using Cinematherapy techniques for eight meetings. The data collection instrument in this study used a scale of student drinking habits which was given at the beginning and end of the treatment and observation of each meeting. The results of the study were in the form of observation sheets and the Wilcoxon Sign Rank Test showing changes in scores before and after treatment. So it can be concluded that the Cinematherapy technique is effective in reducing the habit of consuming liquor among Maumere students at Universitas PGRI Kanjuruhan Malang.
Studi Deskriptif Kebahagiaan pada Mahasiswa Baru
Authors: Herdian Putra Ade , Hariko Rezki
Published: February 2024

Ideally freshmen can adjust to the academic demands of higher education. In addition, freshmen also need to adjust to life as a student university. The inability to adjust in the early days of being a student university has the potential to cause psychological conditions that are not conducive, so that freshmen tend to be at a low level of happiness. This condition if left unchecked can reduce academic achievement and ineffectiveness in living life. The purpose of this research is to describe the happiness of freshmen. This research involved 314 freshmen of the Faculty of Education, Universitas Negeri Padang (UNP) as respondents. The research instrument used is the Happiness Scale. Analysis of the research data used is quantitative descriptive analysis. The results of this study indicate that overall the average value of the happiness level of freshmen is 54.94 with an ideal score of 80. Furthermore, the percentage value of freshmen happiness is 68.67%. This shows that the happiness level of freshmen is in the high category. Furthermore, demographic data on the happiness level of freshmen shows that freshmen of the Guidance and Counseling Study Program are at the highest level of happiness compared to freshmen of other study programs at the Faculty of Education UNP, while the freshmen of the Teacher Education for Early Childhood Education Study Program is at the lowest level of happiness. University counseling centers have an important role in increasing the happiness of freshmen through proper counseling services.
Konseling Behavioral Teknik Relaksasi Afirmasi untuk Menurunkan Academic Burnout, Efektif Tidak?
Authors: Nur Rahmawati Ajeng Intan , Latifah Leny , Saidatun Ica
Published: February 2024

Academic burnout or academic fatigue is a condition experienced by individuals who feel excessive physical fatigue, experience boredom with academics and feel emotional exhaustion caused by the large number of tasks or responsibilities that are so heavy that the individual cannot complete all their responsibilities properly. GoodThe purpose of this research is to determine the effectiveness of behavioral group counseling services using affirmation relaxation techniques to reduce academic burnout. The method used in this research is quasi-experimental design. The technique used for sampling in this research is probability sampling. experimental results of behavioral group counseling services using effective affirmation relaxation techniques to reduce academic burnout.
IMPLICAÇÕES FÍSICAS NO APARELHO ESTOMATOGNÁTICO E EMOCIONAIS NOS ATENDIMENTOS ODONTOLÓGICOS DE MULHERES VÍTIMAS DE VIOLÊNCIA: REVISÃO INTEGRATIVA DA LITERATURA
Authors: Dimas Amanda Londe , Fraga Rhanya Maria Silva , Machado Fabrício Campos , Carvalho Thiago de Amorim
Published: February 2024

Infelizmente os casos de violência contra a mulher aumentam a cada dia. Neste contexto, o entendimento das implicações físicas e emocionais de tais eventos se torna fundamental na equipe multiprofissional de cuidados em saúde, que inclui o cirurgião-dentista. O objetivo deste trabalho foi levantar os principais agravos em saúde bucal relacionados a casos de violência contra a mulher, além de mapear comportamentos que sejam comuns em vítimas de abusos no momento da consulta odontológica. Foi realizada uma revisão integrativa da literatura com buscas nas bases de dados Pubmed e Scielo, de artigos dos últimos cinco anos, buscando responder à seguinte pergunta de estudo: “Quais as principais alterações físicas no sistema estomatognático e as alterações emocionais mais frequentes em mulheres vítimas de violência doméstica?”. Após a aplicação dos critérios de inclusão e exclusão, 13 artigos foram selecionados para a análise. Percebe-se que as lesões em face são comuns em mulheres vítimas de violência, e os dentes não são constantemente acometidos. Em relação às questões emocionais, a consulta odontológica se configura um gatilho de ansiedade para mulheres que sofrem/sofreram algum tipo de violência. Mais estudos são necessários nesta temática que transcende o âmbito jurídico e como uma questão de saúde pública precisa de uma abordagem multi e interdisciplinar, incluindo a Odontologia
HYALURONIC ACID LIP AUGMENTATION AND ITS EFFECT ON HUMAN IDENTIFICATION BY CHEILOSCOPY: A CASE REPORT
Authors: Oliveira Carneiro Daline , Fernandes Gardenia Carvalho Santos , Musse Jamilly de Oliveira , Marques Jeidson Antonio Morais
Published: February 2024

Cheiloscopy is a human identification technique that consists of studying the lips and their characteristics, such as their thickness, the arrangement of the labial commissure, the position of the grooves and their classifications, as well as recording the impressions produced by them on various supports. Lip augmentation with hyaluronic acid (HA) fillers has become very popular in recent years, especially since facial harmonization was included as a dental specialty. The aim of this study was to verify whether lip fillers with HA have implications for cheiloscopic studies, by means of a clinical case report. A 37-year-old female patient with leukoderma took part in the study. Photographs were taken and measurements of lip thickness, lip commissure layout and lipstick impressions were taken before the lip filler, 15 days after the application of 1 ml of HA and 15 days after the full 2 ml of HA filler. The results of the study suggest that lip filling with HA can interfere with the cheiloscopic method, as there was a change in the architecture of the lips, the layout of the labial commissure, improved contour, cupid's bow and filter, with a significant change in the thickness of the upper and lower lips of approximately 42% in relation to the previous volume. As for the pattern of the lip furrows, there was no change in the designs, however, the quality of the lip impression was affected due to the effect produced by the HA
EDITORIAL
Authors: Silva Ricardo Henrique Alves da
Published: February 2024

---
How do Teachers Enhance Elementary School Students’ Numeracy Through the Integration of Singing and Jarimatika Methods?
Authors: Novitasari Welly , Herwin
Published: February 2024

This research aims to reveal teachers' efforts to improve the numeracy of lower grade elementary school students using singing and Jarimatika methods. This research is a type of qualitative research with a case study approach. Research data was collected from elementary school teachers and low-grade elementary school students using observation and interviews. The results of the research show that the teacher's efforts to integrate the singing and Jarimatika methods were carried out using the song "Balonku Ada Lima" whose lyrics were replaced with addition and subtraction rules using the Jarimatika method. Jarimatika rules are taught by singing while simulating number symbols to introduce the concept of place value. Improving number operation skills is carried out by combining the concept of scaffolding, peer tutoring, and drill and practice of calculating using Jarimatika. This combination of methods aims to make it easier for students to strengthen their numeracy skills using Jarimatika method.
Plan de negocios para implementar una empresa de turismo con enfoque en adultos mayores en el departamento de San Martín - Tarapoto
Authors:   , Estrada Casas Lidia Kathya , Feril Condori Gilberto , Mendoza Huamán Karen , Oliva Flores Xiomara , Yarlequé Ramírez Yessenia Rossmery
Published: February 2024

El presente trabajo tiene como objetivo la elaboración de un plan de negocios en el sector de turismo para el segmento de adulto mayor de 60 a más años de edad. Este trabajo es a nivel de perfil de proyecto, desarrollándose bajo un concepto de hipótesis. En la actualidad, el turismo en el Perú está en auge y es reconocida a nivel mundial. Este reconocimiento tuvo inicio en el año 2007, cuando la ciudadela de Machu Picchu fue elegida como una de las 7 maravillas del mundo moderno. Asimismo, la gastronomía también es una razón de la popularidad a nivel mundial de Perú, en los años 2011 y 2013 la gastronomía peruana fue elegida como mejor destino gastronómico del mundo por World Travel Award (WTA). Estos eventos fueron pilares para promocionar al país como un destino privilegiado, para todo turista que buscaba una experiencia llena de lugares paisajísticos, historia, cultura y gastronomía. El departamento de San Martín, en el año 1992 el Parque Nacional del Río Abiseo fue declarado Patrimonio Cultural y Natural de la Humanidad por la UNESCO. San Martín, al encontrarse ubicada en la región de ceja de selva y selva alta, posee innumerables atractivos turísticos, como el Conjunto Arqueológico del Gran Pajatén, Petroglifos de Polish, Cuevas de Juancillo, Siza, el pueblo de Lamas, las Cataratas de Ahuashiyacu, Río Mayo, Lago Lindo, Laguna de Sauce, baños termales y otros. El departamento ocupa el 15 puesto en el índice de competitividad regional. La ciudad de Tarapoto es la principal generadora de turismo para el departamento de San Martín que representa un 55,94% de turistas nacionales y extranjeros, teniendo como principales atractivo turístico Catarata de Ahuashiyacu, Catarata de Pucayaquillo y Alto Shilcayo.
Implementación de una solución de inteligencia de negocios para consolidar información en el área comercial y marketing de la empresa corporación Primax
Authors:   , Moreno Nolazco Roberto Carlos
Published: February 2024

En la actualidad la información se considera uno de los principales recursos dentro de una organización. Muchas empresas buscan maximizar la utilidad que posee la información para ser conducidos a una óptima toma de decisiones que contribuya con la estrategia y ventaja competitiva. Sin embargo, esto resulta muy cuando la organización no cuenta con herramientas de Inteligencia de Negocios que faciliten la explotación de la información en las áreas estratégicas. Gartner (2017) señala que: “Se conoce que en la actualidad existe mayor prioridad en las organizaciones frente a las soluciones de inteligencia de negocios y a las técnicas que permiten extraer y analizar la información de manera óptima”. Las tareas que involucren análisis de datos se realizarán por medio de máquinas en más del 40% para el 2020. Incluso, predijo que para ese mismo año las interacciones entre la organización y los clientes estarán influenciados en un 30% para el análisis que se realicen a los datos en base al comportamiento, preferencia y ubicación en tiempo real. (Gartner, 2017). Kimball (2002) afirma “Con una óptima solución de inteligencia de negocios se puede administrar múltiples procesos en una organización”. De esta manera se puede incrementar la productividad y lograr reducir gastos a nivel de procesos y horas hombre.
Evaluación clínica y radiográfica de la respuesta del tejido pulpar expuesta a la proteína derivada de la matriz de esmalte como agente en pulpotomías en molares deciduas a las 12 meses: reportes de caso
Authors:   , Abal Perleche Dessire Milagros
Published: February 2024

La pulpotomía es un procedimiento en donde se retira la parte infectada coronal de la pulpa vital seguida de la aplicación del medicamento sobre el tejido para poder promover la curación. El medicamento debe tener ciertas características como: bactericida, no lesivo, formar una barrera dentinaria, promover la cicatrización de la pulpa, y no debe afectar la reabsorción fisiológica de la raíz del diente deciduo. La Proteína derivada de la matriz de esmalte (EMDOGAIN) es un material bioactivo, el cual estimula la regeneración de los tejidos periodontales, la proteína de la matriz, ligamento periodontal, cemento y hueso alveolar. Este material fue utilizado en los 5 casos de tratamientos de pulpotomía en molares deciduas. La evaluación post tratamiento fue clínica y radiográfica al mes, 6 meses y 1 año, y en todos los casos evaluados se observó ausencia de sintomatología clínica y radiográfica y se verificó la preservación de la vitalidad pulpar después de 1, 6 y 12 meses de seguimiento.
Ecological dynamics of anoxygenic microorganisms in stably redox-stratified waters: Intra and inter-seasonal variability of Lake Cadagno
Authors: Storelli Nicola , Steiner Oscar Sepuveda , Nezio Francesco Di , Roman Samuele , Buetti-Dinh Antoine , Bouffard Damien
Published: February 2024

Lake Cadagno (Switzerland) differs from common alpine lakes, as it consists of two stratified layers of water that never vertically mix. This particular environment is a niche for the development of ancient anoxygenic microorganisms similar to those that populated the primordial oceans – which accumulate in the lower part of the chemocline. Yet, linkages between the temporal variation of abiotic factors, controlling stratification, with anoxygenic phototrophic sulfur bacteria community of the bacterial layer (BL) remain unclear. We studied the intra- and inter-seasonal stability of the water column stratification and population dynamics of the BL for three years to address this gap. Analyses of this extended continuous monitoring show the redox-stratified environments of the lake is remarkably stable, with stratification seemingly unaffected by abiotic factors. In contrast, the BL community showed significant intra- and inter-seasonal variability. The distinct weather conditions observed during these three years strongly influenced the composition and development of the BL community. Indeed, especially at the intra-seasonal level, the main populations monitored showed different dynamics each year. The lake's recent history also revealed the same trend. In fact, over the last 35 years, the stability of the meromixis remained unchanged, while the BL community showed considerable variability.
Brain connectome from neuronal morphology
Authors: Wang Jinhui , Jin Suhui , Li Junle
Published: February 2024

Morphological brain networks derived from macroscopic structural magnetic resonance imaging have become prevalent, yet lack microscopic validation. Here, we proposed a method to construct morphological brain networks at the single-cell level by estimating inter-neuron similarity for rat, mouse, and human. We demonstrated the feasibility and generalizability of the method by showing that inter-neuron morphological similarity was correlated with neuronal axonal projections, was higher for intra- than inter-class connections, depended on cytoarchitectonic, chemoarchitectonic, and laminar structures of neurons, and differed between regions with different evolutionary timelines. Furthermore, highly connected hub neurons were disproportionately located in superficial layers, inhibitory neurons, and subcortical regions, and exhibited unique morphology. Finally, we demonstrated a more segregated, less integrated, and economic network architecture with worse resistance to targeted attacks for the human than mouse. Overall, our findings provide microscopic support for using structural magnetic resonance imaging-based morphological brain networks to study the wiring patterns in brains.
Semantic Inhibition is impaired in college students with depressive traits. EEG and pupillometric evidence with the Hayling’s task
Authors: Jan Damián , López-Pigüi Joana , Padrón Iván , de Vega Manuel
Published: February 2024

In this study we tested whether depression is associated with impaired semantic inhibition, resulting in symptoms of rumination and anhedonia. To this aim, college students with depressive traits (DEP) and matched controls (CTL) performed a Hayling’s task, while EEG and pupillometry measures were recorded. Participants were asked to complete sentential contexts with either a highly associated word (initiation) or a non-related word (inhibition), in response to randomly presented trial-by-trial cues. The DEP group, compared to the CTL group, showed lower performance, and reduced frontal negativity (N450) in inhibition trials. Source analyses revealed greater activation for inhibition trials than for initiation trials in the bilateral orbitofrontal cortex for the CTL group, but the difference was reduced and right lateralized for the DEP group. In addition, the DEP group showed more pupil size reactivity to inhibition trials than the CTL group, indicating higher cognitive effort during semantic inhibition. Finally, self-reported rumination and anhedonia correlated with N450 in inhibition trials. Overall, this research contributes to understanding the neural underpinnings of impaired semantic inhibition in subclinical depressive participants, with potential clinical applications.
The correlation between Framingham risk score and hypertension combined with hyperuricemia in an elderly community-based population--a 7-year cohort follow-up study
Authors: Shen Chaonan , Li Xin , Li Weipeng , Liu Jie , Zhang Yuzhen , Liu Zhongmin , Zheng Liang
Published: February 2024

Background
Research has consistently demonstrated a robust association between hypertension (HTN) and hyperuricemia (HUA), indicating a potential synergistic effect on cardiovascular disease (CVD) risk. This study aims to explore the epidemiological characteristics of hypertension combined with hyperuricemia (HTN-HUA) and to identify the risk factors contributing to its incidence.
Methods
This study was a cohort follow-up, utilizing a community-based cohort consisting of older adults aged 65 years or older. Initially, the study enrolled 3,950 participants in a prospective manner. After the exclusion of cases with missing data, the analysis ultimately included 3,089 participants. We employed the Framingham Risk Score (FRS) formula to estimate the risk of CVD. Survival analysis was conducted to explore the relationship between FRS and CVD death/all-cause mortality. Furthermore, multiple logistic regression models were utilized to assess the association between FRS and HTN-HUA.
Results
Within the study population, we found that approximately 29.6% of hypertensive patients were also suffering from HUA. We observed that regardless of the presence of HTN or HUA, age, WC, SBP, DBP, Scr, and UA levels were significantly higher in the High-risk group than in the L-M risk group (P < 0.05). The Kaplan-Meier survival analysis, which used CVD death as the endpoint, revealed a significant difference in survival curves between the High-risk and low-intermediate (L-M) risk groups among relatively healthy individuals (P < 0.01). Furthermore, following adjustments for confounding variables, logistic regression analysis revealed that the risk of HTN-HUA in the High-risk group was 1.485 times greater than in the L-M risk group (OR = 1.485, 95% CI: 1.092–2.021).
Conclusion
In a community-based elderly population in China, the prevalence of HTN-HUA was found to be 17.5%. The FRS effectively predicted a 10-year cardiovascular risk in a relatively healthy population. Additionally, waist circumference (WC) and body mass index (BMI) were identified as significant risk factors for the development of HTN-HUA.
Regeneration of the Caudal Fin of Atractosteus tropicus The Tropical Gar
Authors: Rios-Flores Alberto J. , López-Flores Sandra , Martínez-Moreno Jose A. , Falcon-Romero Karen Y. , Ascencio-Alcudia Gloria G. , Sepúlveda-Quiroz Cesar A. , Martínez-García Rafael , González Carlos A. Alvarez , Rodríguez-Salazar Elizabeth , Maldonado Ernesto
Published: February 2024

Bacground:
The tropical gar (Atractosteus tropicus) belongs to the lepisosteidae family, with habitats from southeast Mexico to the south of Costa Rica. Due to its phylogenetic position, slow evolution speed (base substitution), dense genetic map, gene similarity with human beings, and ease of cultivation in laboratories, this organism provides a unique link between tetrapods and the biomedical models of teleosts. This fish is a taxonomic sister group to teleosts such as the zebrafish (Danio reiro), which possesses a high regenerative ability; however, it is not known if the tropical gar shows this same regeneration ability and whether it is similar or identical.
Results
This study aims to demonstrate the regeneration process of the caudal fin through skeletal and histological staining. For this reason, several juvenile organisms were studied for about two months, fed with artemia (Artemiidae), and anesthetized by immersion with 1% eugenol to carry out the amputation of the caudal fin and obtain the daily samples at different time points or dpa (days post-amputation) dpa. The skeleton was stained with alcian blue and alizarin red to observe the regeneration of the fin by the growth of new cartilage. Histological staining with hematoxylin and eosin was also carried out. Histological sections were made from each sample and stained, observing the regeneration on the uppermost part of the amputation.
Conclusions
The findings show are remarkable ability for caudal fin regeneration in juvenile individuals from the tropical Gar. Since Garfish are the phylogenetically sister group of teleost fish it offers an opportunity for future research in tissue regeneration.
Arabidopsis endoplasmic reticulum-localized acyltransferase LPAT2 interacts with ATI1/2 and mediates lipid droplet proliferation
Authors: Zhang Qun , Li Wenyan , Liu Wenbao , Yuan Siqi , Liao Qi , Jia Qianru , Li Xuebing , Yuan Jingya , Zhang Wenhua
Published: February 2024

Lipid droplet (LD) biogenesis from the endoplasmic reticulum (ER) is accompanied by conversion of phospholipids to triacylglycerol and expansion of phospholipid monolayer surface. The determinants of this process are largely unknown in plants. Here, we demonstrate that the Arabidopsis thaliana lysophosphatidic acid acyltransferase, LPAT2, and its product phosphatidic acid (PA), are critical lipid-based cues for LD proliferation. RNAi suppression of LPAT2decrease PA accumulation at the monolayer surface of LDs, consequently compromising dark- or stress-induced LD biogenesis, whereas overexpression of LPAT2 exhibits opposite effects. Furthermore, LPAT2 physically interacts with ATG8-interacting protein 1/2 (ATI1/2), which serve as autophagy cargo receptors. Mutations of ATI1/2 drastically disrupted LPAT2 translocation from the ER to the forming LDs. Our data suggest that the LPAT2-ATI1/2 complex is the core protein machinery that facilitates LD biogenesis to mediate plant responses to developmental and environmental stimuli.
Locals' perceptions, knowledge, and attitudes regarding the conservation of the critically endangered Phataginus tricuspis; Reflections on OECM Sites in Osun State, Nigeria
Authors: OWOLABI Bibitayo Ayobami , AKINSOROTAN Oluseun Adesola , ADEWUMI Abraham Adebayo Adewumi , SANUADE Adedamola Oyinade
Published: February 2024

This study evaluates local communities' knowledge, attitude, and perception towards the critically endangered African Tree Pangolin and its conservation in the 15 identified Other Effect Area Based Conservation Measures (OECM) sites in Osun State, Nigeria. 360 participants aged 20 and older participated in the study, which found that 90.33% supported the presence of African Tree Pangolin. However, 61% affirmed that their population is reducing due to hunting and habitat loss from poor agriculture. 67% of interviewees believed that benefits from wildlife through tourism, insect regulation, bush meat, and medicine influenced their attitudes and perceptions towards conservation. 33% of the local population changed their attitude and perception towards conservation. African Tree Pangolin conservation was deemed a priority by those with higher levels of education, but the majority of respondents claimed that the idea was unsupported because of the high revenue generated by Pangolin sales. Increasing public awareness through media, handbills, billboards, seminars, and films could help mitigate poor attitudes towards African Tree Pangolin conservation.
Human Umbilical Cord Mesenchymal Stem Cell-Based Gene Therapy for Hemophilia B using scAAV-DJ/8-LP1-hFIXco Transduction
Authors: Bu Zibin , Lou Jintu , Xu Weiqun , Zhang Lingyan , Tang Yongmin
Published: February 2024

Background
Hemophilia B is an X-linked bleeding disorder caused by a mutation in the gene responsible for encoding coagulation factor IX (FIX). Gene therapy offers promising potential for curing this disease. However, the current method of relatively high dosage of virus injection carries inherent risks. The purpose of this study was to introduce a novel scAAV-DJ/8-LP1-hFIXco vector transduced human umbilical cord blood derived mesenchymal stem cells (HUCMSCs) as an alternative cell-based gene therapy to conventional gene therapy for Hemophilia B.
Methods
The human FIX activation system was employed for detection. The RNA and protein expression levels were evaluated using PCR and western blot techniques. In animal studies, clotting time was utilized as a parameter for bleeding assessment. The immunohistochemical analysis was used to assess the distribution of HUCMSCs. The safety of this cell-based gene therapy was evaluated using hematoxylin-eosin staining.
Results
Our findings demonstrate that transduction of HUCMSCs with the scAAV-DJ/8-LP1-hFIXco vector results in consistent and sustainable secretion of human FIX both in vitro and in vivo. The secretion level is comparable to that observed following intravenous injection with a high dose of the viral vector. After a 5-month observation period, no tumor-related tissues were observed in any of the mice studied.
Conclusions
we have successfully developed a novel cell-based gene therapy for the potential and safer treatment option for Hemophilia B.
Impact of clinical clerkship integrated with clinical ladder on attending physicians’ self-efficacy
Authors: Arai Yuto , Yoshino Go , Ohta Kento , Okanishi Tohru , Kakee Sosuke , Mino Yoichi , Komatsu Hiroaki , Yamada Nanako , Ueki Masaru , Maegaki Yoshihiro
Published: February 2024

Background
Self-efficacy plays an important role in enhancing the teaching capabilities of attending physicians (APs). The clinical ladder (CL) is an educational approach developed in the field of nursing education that increases difficulty in an incremental manner. However, no previous study has confirmed the effectiveness of CL in medical education. Therefore, this study aimed to examine the effect of clinical clerkship integrated with clinical ladder (CC-CL) on the self-efficacy of APs.
Methods
Sixth year medical students participated in CC-CL for 6 months from April 2023, and the changes in the self-efficacy of APs were retrospectively evaluated. The students were trained by the APs concurrently, and the achievement levels of each student were shared. The primary outcome measure was the physician teaching self-efficacy questionnaire (PTSQ) score. The PTSQ scores before and after CC-CL were analyzed using the Wilcoxon matched-pair signed-rank test.
Results
Fifteen APs from the Department of Pediatric and Child Neurology were included in this study. No significant difference was observed in the total PTSQ scores of the APs before and after CC-CL. However, a significant increase was observed in the PTSQ score of APs who participated for a duration of 2 hours per week for > 3 months (n = 8) after CC-CL (p = 0.022). Furthermore, APs who had received their pediatrician certification < 10 years prior (n = 8) showed a significant increase in the total PTSQ score after CC-CL (p = 0.022).
Conclusions
CC-CL may play an important role in cultivating the self-efficacy of less experienced APs. Further comparative studies must be conducted in the future to validate the findings of this study.
RAB18 Regulates Extrahepatic SiRNA-Mediated Gene Silencing Efficacy
Authors: Lu Jiamiao , Lee Jasper , Yuan Eric , Wakefield Devin , Kanke Matt , Pruitt Danielle , Barreda Jose , Rulifson Ingrid , Xie Jiansong , Ferbas John , Long Jason , Meade Bryan , Homann Oliver , Guo Wei , Gomes Tina , Zhou Hong , Wu Bin , Cui Jixin , Wang Songli
Published: February 2024

Small interfering RNAs (siRNAs) hold considerable therapeutic potential to selectively silence previously “undruggable” disease-associated targets, offering new opportunities to fight human diseases. This therapeutic strategy, however, is limited by the inability of naked siRNAs to passively diffuse across cellular membranes due to their large molecular size and negative charge. Delivery of siRNAs to liver through conjugation of siRNA to N-acetylgalactosamine (GalNAc) has been a success; providing robust and durable gene knockdown specifically in hepatocytes. Yet, the poor delivery and silencing efficacy of siRNAs in other cell types has hindered their applications outside the liver. We previously reported that a genome-wide CRISPR screen identified RAB18 as a major modulator of GalNAc-siRNA conjugates. Herein, we demonstrate RAB18 knockout/knockdown efficaciously enhances siRNA-mediated gene silencing in hepatic, extrahepatic cell lines, and in vivo. Our results reveal a mechanism by which retrograde Golgi-ER transport and the intracellular lipid droplets (LDs) positively regulate siRNA-mediated gene silencing.
Longitudinal Association between Stressful Life Events and Suicidal Ideation in Adults with Major Depression Disorder: The Mediating Effects of Insomnia Symptom
Authors: chen ya , Han Xue , Jiang Yingchen , Jiang Yunbin , Huang Xinyu , Wang Wanxin , Guo Lan , Xia Ruirui , Liao Yuhua , Zhang Huimin , Teopiz Kayla M. , McIntyre Roger S. , Fan Beifang , Lu Ciyong
Published: February 2024

Background: Stressful life events (SLEs) and suicidal ideation (SI) are prevalent in persons with major depression disorder (MDD). Less is known about the underlying role of insomnia symptoms in the association between SLEs and SI. This study sought to investigate the longitudinal association among SLEs, insomnia symptoms and SI in persons with MDD.
Methods: This three-wave prospective study used data collected between December 2020 and September 2022 from the Depression Cohort in China (DCC), which is a large, ongoing cohort study targeting at persons aged 18-64 years with MDD. The study population included 511 persons with MDD (mean [SD] age, 28.7 [6.7] years, 67.1% were females). A structured questionnaire and validated scales were utilized to assess the cumulative numbers and multiple types of SLEs, insomnia symptoms and SI. Generalized estimated equation (GEE) was utilized to explore prospective association among exposure of SLEs, insomnia symptoms and SI. Besides, the structural equation model (SEM) was performed to estimate the longitudinal mediating effect of insomnia symptoms in the relationship between SLEs and SI.
Results: After adjusting for control variables, three or more SLEs (adjusted β estimate = 1.299, 95% CI=1.016-1.662) and childhood physical abuse (adjusted βestimate = 1.301, 95% CI=1.020-1.658) were related to an elevated risk of SI. Additionally, the association between SLEs and SI was significantly mediated by insomnia symptoms (standardized β estimate = 0.026, 95% CI = 0.003 - 0.053).
Conclusions: Cumulative SLEs were determined to be longitudinally associated with SI in persons with MDD. We further observed that insomnia symptoms were highly associated with SLEs and SI. Clinicians assessing persons with MDD, especially with history of SLE and insomnia symptoms, should carefully evaluate for the presence of suicidality as part of a personalized assessment of their depressive illness.
Gohteikhimyces, a novel hyphomycete genus from submerged wood, based on three collections in Taiwan
Authors: Ou Jie-Hao , Hsieh Sung-Yuan , Kuo Chang-Hsin
Published: February 2024

Gohteikhimyces, a new genus of dematiaceous hyphomycete occurring on decaying wood submerged in a freshwater stream of Taiwan, is proposed based on morphological and molecular data. It produces effuse, black, and somewhat glistening colonies on the surface of natural substratum, which mainly composed of solitary, dry, dark, cymbiform or ellipsoidal to obovoid, 3–4-euseptate conidia, and secede rhexolytically from semi-macronematous conidiophores with percurrent regeneration. The conidia resemble those of Bactrodesmium species but they are not produced from sporodochial conidiomata. Phylogenetically, this new genus is placed in the Savoryellomycetidae incertae sedis, closely related to Flammispora, but is distant from the Fucosporellales which contains the Bactrodesmium species. This new genus is proposed based on three collections from different localities in Taiwan, with descriptions of two novel species, G. bactrodesmioides and G. taroides, which differ in geographical conditions of collection sites and conidial morphology. The two species are supported by sufficient differences in their LSU, SSU, and the ITS sequences. In this paper, the genus Gohteikhimyces is illustrated with line diagrams, light micrographs, and scanning electron micrographs.
Safety of Physician-staffed Medical Service in Rendering Prehospital emergency medicine among Assault Cases in Japan: A Single-center, Retrospective, Case-Control Study
Authors: Kikuta Shota , Kai Soichiro , Nakayama Haruki , Matsuyama Shigenari , Kawase Tetsunori , Ishihara Satoshi
Published: February 2024

Purpose
The current study aimed to investigate assault cases involving prehospital emergency care, focusing on safety aspects.
Methods
This single-center, retrospective case-control study reviewed our facility’s physician-staffed medical care, including consecutive assault cases to patients injured by a perpetrator's intentional attack from January 2005 to October 2020. The study relied on our institution’s medical and prehospital emergency care and communication records with the fire department. The patients were divided into two groups, “problem group” and “control group”, based on the viewpoint of operational safety.
Results
Of the 4,957 prehospital emergency care cases during the study period, 65 (1.3%) were identified as assault cases, with 10 in the problem group and 55 in the control group. A multivariate analysis using logistic regression analysis revealed non-penetrating injury (odds ratio [OR]: 6.97, 95% confidence interval [CI]: 1.37–35.4, P = 0.019) and shorter interval from the request for physician dispatch to contact with the injured person (request-to-contact interval) (OR: 0.762, 95% CI: 0.580–0.996, P = 0.047) as the independent risk factors to predict the problem group.
Conclusion
The attack method was a penetrating mechanism in most of the assault cases. Conversely, cases with non-penetrating injuries or shorter request-to-contact interval may cause problems in prehospital emergency care and therefore require particular attention.
X-ray Chemical Imaging of Mn-doped Co/TiO2 Pellets reveals the significance of Cobalt Carbide Formation and Distribution on CO conversion and Alcohol Selectivity during Fischer Tropsch Synthesis
Authors: Beale Andrew , Farooq Danial , Potter Matthew , Stockenhuber Sebastian , Pritchard Jay , Vamvakeros Antonis , Price Stephen , Drnec Jakub , Ruchte Ben , Paterson James , Peacock Mark
Published: February 2024

X-ray diffraction/scattering computed tomography (XRS-CT) were used to create 2D images, with 20 µm resolution, of passivated Co/TiO2/Mn Fischer Tropsch catalyst extrudates post-reaction after 300 h on stream under industrially relevant conditions. This combination of scattering techniques provided unprecedented insights into both the spatial variation of the various cobalt phases and the influence of increasing Mn loading has on this. Also observed is the presence of a wax coating throughout the extrudate and its capacity to preserve the Co/Mn species in the same state as in the reactor. Correlating these findings with catalytic performance, highlights the crucial phases and active sites within Fischer Tropsch catalysts required for understanding the tunability of the product distribution between saturated hydrocarbons or oxygenate and olefin products. In particular a Mn loading of 3 wt. % led to an optimum equilibrium between the amount of hexagonal close-packed Co and Co2C phases resulting in maximum oxygenate selectivity. XRS-CT revealed Co2C to be located on the extrudates' periphery, while metallic Co phases were more prevalent towards the centre, possibly due to a lower [CO] ratio there. Reduction at 450°C of a 10 wt. % Mn sample resulted in MnTiO3 formation which inhibited carbide formation and alcohol selectivity. It is suggested that small MnO particles promote Co carburisation by decreasing the CO dissociation barrier and the Co2C phase promotes CO non-dissociative adsorption leading to increased oxygenate selectivity. This study highlights the influence of Mn on catalyst structure and function and the importance of studying catalysts under industrially relevant reaction times.
A novel spatial-frequency feature representation network for zero-shot incremental learning
Authors: Ren Jie , Zhao Yang , Zhang Weichuan , Sun Changming
Published: February 2024

Zero-shot incremental learning aims to enable the model to generalize to new classes without forgetting previously learned classes. However, the semantic gap between old and new sample classes can lead to catastrophic forgetting. Additionally, existing algorithms lack capturing significant information from each sample image domain, impairing models’ classification performance. Therefore, this paper proposes A novel Spatial-Frequency Feature Representation Network (SFFRNet) which contains a Spatial-Frequency Feature Extraction (SFFE) module and Attention Feature Alignment (AFA) module to improve the Zero-Shot Translation for Class Incremental algorithm. Firstly, SFFE module is designed which contains a dual attention mechanism for obtaining salient spatial-frequency feature information. Secondly, a novel feature fusion module is conducted for obtaining fused spatial-frequency domain features. Thirdly, the Nearest Class Mean classifier is utilized to select the most suitable category. Finally, iteration between tasks is performed using the Zero-Shot Translation model. The proposed SFFRNet has the ability to effectively extract spatial-frequency feature representation from input images, improve the accuracy of image classification, and fundamentally alleviate catastrophic forgetting. Extensive experiments on the CUB 200-2011 and CIFAR-100 datasets demonstrate that our proposed algorithm outperforms state-of-the-art incremental learning algorithms.