bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural Mapping of Missense Mutations in the Pex1/Pex6 Complex
Authors: Schieferdecker Anne , Wendler Petra
Published: August 2019

Peroxisome biogenesis disorders (PBDs) are nontreatable hereditary diseases with a broad range of severity. Approximately 65% of patients are affected by mutations in the peroxins Pex1 and Pex6. The proteins form the heteromeric Pex1/Pex6 complex, which is important for protein import into peroxisomes. To date, no structural data are available for this AAA+ ATPase complex. However, a wealth of information can be transferred from low-resolution structures of the yeast scPex1/scPex6 complex and homologous, well-characterized AAA+ ATPases. We review the abundant records of missense mutations described in PBD patients with the aim to classify and rationalize them by mapping them onto a homology model of the human Pex1/Pex6 complex. Several mutations concern functionally conserved residues that are implied in ATP hydrolysis and substrate processing. Contrary to fold destabilizing mutations, patients suffering from function-impairing mutations may not benefit from stabilizing agents, which have been reported as potential therapeutics for PBD patients.
Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai (Brassica campestris L.) Genotype
Authors: Yuan Lingyun , Wang Jie , Xie Shilei , Zhao Mengru , Nie Libing , Zheng Yushan , Zhu Shidong , Hou Jinfeng , Chen Guohu , Wang Chenggang
Published: August 2019

The genotype WS-1, previously identified from novel wucai germplasm, is tolerant to both low-temperature (LT) and high-temperature (HT) stress. However, it is unclear which signal transduction pathway or acclimation mechanisms are involved in the temperature-stress response. In this study, we used the proteomic method of tandem mass tag (TMT) coupled with liquid chromatography-mass spectrometry (LC-MS/MS) to identify 1022 differentially expressed proteins (DEPs) common to WS-1, treated with either LT or HT. Among these 1022 DEPs, 172 were upregulated in response to both LT and HT, 324 were downregulated in response to both LT and HT, and 526 were upregulated in response to one temperature stress and downregulated in response to the other. To illustrate the common regulatory pathway in WS-1, 172 upregulated DEPs were further analyzed. The redox homeostasis, photosynthesis, carbohydrate metabolism, heat-shockprotein, and chaperones and signal transduction pathways were identified to be associated with temperature stress tolerance in wucai. In addition, 35S:BcccrGLU1 overexpressed in Arabidopsis, exhibited higher reduced glutathione (GSH) content and reduced glutathione/oxidized glutathione (GSH/GSSG) ratio and less oxidative damage under temperature stress. This result is consistent with the dynamic regulation of the relevant proteins involved in redox homeostasis. These data demonstrate that maintaining redox homeostasis is an important common regulatory pathway for tolerance to temperature stress in novel wucai germplasm.
Gene Expression Profiling Reveals that PXR Activation Inhibits Hepatic PPARα Activity and Decreases FGF21 Secretion in Male C57Bl6/J Mice
Authors: Barretto Sharon Ann , Lasserre Frédéric , Fougerat Anne , Smith Lorraine , Fougeray Tiffany , Lukowicz Céline , Polizzi Arnaud , Smati Sarra , Régnier Marion , Naylies Claire , Bétoulières Colette , Lippi Yannick , Guillou Hervé , Loiseau Nicolas , Gamet-Payrastre Laurence , Mselli-Lakhal Laila , Ellero-Simatos Sandrine
Published: August 2019

The pregnane X receptor (PXR) is the main nuclear receptor regulating the expression of xenobiotic-metabolizing enzymes and is highly expressed in the liver and intestine. Recent studies have highlighted its additional role in lipid homeostasis, however, the mechanisms of these regulations are not fully elucidated. We investigated the transcriptomic signature of PXR activation in the liver of adult wild-type vs. Pxr-/- C57Bl6/J male mice treated with the rodent specific ligand pregnenolone 16α-carbonitrile (PCN). PXR activation increased liver triglyceride accumulation and significantly regulated the expression of 1215 genes, mostly xenobiotic-metabolizing enzymes. Among the down-regulated genes, we identified a strong peroxisome proliferator-activated receptor α (PPARα) signature. Comparison of this signature with a list of fasting-induced PPARα target genes confirmed that PXR activation decreased the expression of more than 25 PPARα target genes, among which was the hepatokine fibroblast growth factor 21 (Fgf21). PXR activation abolished plasmatic levels of FGF21. We provide a comprehensive signature of PXR activation in the liver and identify new PXR target genes that might be involved in the steatogenic effect of PXR. Moreover, we show that PXR activation down-regulates hepatic PPARα activity and FGF21 circulation, which could participate in the pleiotropic role of PXR in energy homeostasis.
Propolis Reduces the Expression of Autophagy-Related Proteins in Chondrocytes under Interleukin-1β Stimulus
Authors: Arias Consuelo , Saavedra Nicolás , Saavedra Kathleen , Alvear Marysol , Cuevas Alejandro , Stuchi Maria-Engler Silvya , Abdalla Dulcineia S. P. , Salazar Luis A.
Published: August 2019

Background: Osteoarthritis (OA) is a progressive and multifactorial disease that is associated with aging. A number of changes occur in aged cartilage, such as increased oxidative stress, decreased markers of healthy cartilage, and alterations in the autophagy pathway. Propolis extracts contain a mixture of polyphenols and it has been proved that they have high antioxidant capacity and could regulate the autophagic pathway. Our objective was to evaluate the effect of ethanolic extract of propolis (EEP) on chondrocytes that were stimulated with IL-1β. Methods: Rabbit chondrocytes were isolated and stimulated with IL-1β and treated with EEP. We evaluated cell viability, nitric oxide production, healthy cartilage, and OA markers, and the expression of three proteins associated with the autophagy pathway LC3, ATG5, and AKT1. Results: The EEP treatment reduces the expression of LC3, ATG5, and AKT1, reduces the production of nitric oxide, increases the expression of healthy markers, and reduces OA markers. Conclusions: These results suggest that treatment with EEP in chondrocytes that were stimulated with IL-1β has beneficial effects, such as a decrease in the expression of proteins associated with autophagy, MMP13, and production of nitric oxide, and also increased collagen II.
Impact of Biotic and Abiotic Stressors on Managed and Feral Bees
Authors: Belsky Joseph , Joshi Neelendra K.
Published: August 2019

Large-scale declines in bee abundance and species richness over the last decade have sounded an alarm, given the crucial pollination services that bees provide. Population dips have specifically been noted for both managed and feral bee species. The simultaneous increased cultivation of bee-dependent agricultural crops has given rise to additional concern. As a result, there has been a surge in scientific research investigating the potential stressors impacting bees. A group of environmental and anthropogenic stressors negatively impacting bees has been isolated. Habitat destruction has diminished the availability of bee floral resources and nest habitats, while massive monoculture plantings have limited bee access to a variety of pollens and nectars. The rapid spread and increased resistance buildup of various bee parasites, pathogens, and pests to current control methods are implicated in deteriorating bee health. Similarly, many pesticides that are widely applied on agricultural crops and within beehives are toxic to bees. The global distribution of honey bee colonies (including queens with attendant bees) and bumble bee colonies from crop to crop for pollination events has been linked with increased pathogen stress and increased competition with native bee species for limited resources. Climatic alterations have disrupted synchronous bee emergence with flower blooming and reduced the availability of diverse floral resources, leading to bee physiological adaptations. Interactions amongst multiple stressors have created colossal maladies hitting bees at one time, and in some cases delivering additive impacts. Initiatives including the development of wild flower plantings and assessment of pesticide toxicity to bees have been undertaken in efforts to ameliorate current bee declines. In this review, recent findings regarding the impact of these stressors on bees and strategies for mitigating them are discussed.
Fucoxanthin and Rosmarinic Acid Combination Has Anti-Inflammatory Effects through Regulation of NLRP3 Inflammasome in UVB-Exposed HaCaT Keratinocytes
Authors: Rodríguez-Luna Azahara , Ávila-Román Javier , Oliveira Helena , Motilva Virginia , Talero Elena
Published: August 2019

Excessive exposure to ultraviolet (UV) radiation is the main risk factor to develop skin pathologies or cancer because it encourages oxidative condition and skin inflammation. In this sense, strategies for its prevention are currently being evaluated. Natural products such as carotenoids or polyphenols, which are abundant in the marine environment, have been used in the prevention of oxidative stress due to their demonstrated antioxidant activities. Nevertheless, the anti-inflammatory activity and its implication in photo-prevention have not been extensively studied. Thus, we aimed to evaluate the combination of fucoxanthin (FX) and rosmarinic acid (RA) on cell viability, apoptosis induction, inflammasome regulation, and anti-oxidative response activation in UVB-irradiated HaCaT keratinocytes. We demonstrated for the first time that the combination of FX and RA (5 µM RA plus 5 μM FX, designated as M2) improved antioxidant and anti-inflammatory profiles in comparison to compounds assayed individually, by reducing UVB-induced apoptosis and the consequent ROS production. Furthermore, the M2 combination modulated the inflammatory response through down-regulation of inflammasome components such as NLRP3, ASC, and Caspase-1, and the interleukin (IL)-1β production. In addition, Nrf2 and HO-1 antioxidant genes expression increased in UVB-exposed HaCaT cells pre-treated with M2. These results suggest that this combination of natural products exerts photo-protective effects by down-regulating NRLP3-inflammasome and increasing Nrf2 signalling pathway.
Facile Fabrication of Conductive Graphene/Polyurethane Foam Composite and Its Application on Flexible Piezo-Resistive Sensors
Authors: Zhong Weibing , Ding Xincheng , Li Weixin , Shen Chengyandan , Yadav Ashish , Chen Yuanli , Bao Mingze , Jiang Haiqing , Wang Dong
Published: August 2019

Flexible pressure sensors have attracted tremendous research interests due to their wide applications in wearable electronics and smart robots. The easy-to-obtain fabrication and stable signal output are meaningful for the practical application of flexible pressure sensors. The graphene/polyurethane foam composites are prepared to develop a convenient method for piezo-resistive devices with simple structure and outstanding sensing performance. Graphene oxide was prepared through the modified Hummers method. Polyurethane foam was kept to soak in the obtained graphene oxide aqueous solution and then dried. After that, reduced graphene oxide/polyurethane composite foam has been fabricated under air phase reduction by hydrazine hydrate vapor. The chemical components and micro morphologies of the prepared samples have been observed by using FT-IR and scanning electron microscopy (SEM). The results predicted that the graphene is tightly adhered to the bare surface of the pores. The pressure sensing performance has been also evaluated by measuring the sensitivity, durability, and response time. The results indicate that the value of sensitivity under the range of 0–6 kPa and 6–25 kPa are 0.17 kPa−1 and 0.005 kPa−1, respectively. Cycling stability test has been performed 30 times under three varying pressures. The signal output just exhibits slight fluctuations, which represents the good cycling stability of the pressure sensor. At the same stage, the response time of loading and unloading of 20 g weight turned out to be about 300 ms. These consequences showed the superiority of graphene/polyurethane composite foam while applied in piezo-resistive devices including wide sensitive pressure range, high sensitivity, outstanding durability, and fast response.
Corrosion Detection by Infrared Attenuated Total Reflection Spectroscopy via Diamond-Like Carbon-Coated Silicon Wafers and Iron-Sensitive Dyes
Authors: Türkmen Dervis , Dettenrieder Carina , Forsberg Pontus , Mattsson Andreas , Nikolajeff Fredrik , Österlund Lars , Karlsson Mikael , Mizaikoff Boris
Published: August 2019

The durability of metal-based constructions, especially those containing reinforced concrete, is mainly limited by corrosion processes. Diamond-like carbon (DLC)-coated silicon (Si) wafers provide a chemically inert and mechanically robust sensing interface for application in aggressive environments. In this study, iron-sensitive dyes, i.e., 2,3-dihydroxypyridine (DHP) and 1,2-dihydroxybenzol (DHB), were coated onto DLC-modified Si wafers for evaluating the potential of detecting corrosion processes via evanescent field absorption spectroscopy using Fourier-transform infrared spectroscopy. The obtained IR spectra reveal discernible changes of the dye layer after exposure to iron solutions, which indicates that indeed corrosion processes may be studied at molecular level detail.
IoT Enabled Intelligent Sensor Node for Smart City: Pedestrian Counting and Ambient Monitoring
Authors: Akhter Fowzia , Khadivizand Sam , Siddiquei Hasin , Alahi Md , Mukhopadhyay Subhas
Published: August 2019

An Internet of Things (IoT) enabled intelligent sensor node has been designed and developed for smart city applications. The fabricated sensor nodes count the number of pedestrians, their direction of travel along with some ambient parameters. The Field of View (FoV) of Fresnel lens of commercially available passive infrared (PIR) sensors has been specially tuned to monitor the movements of only humans and no other domestic animals such as dogs, cats etc. The ambient parameters include temperature, humidity, pressure, Carbon di Oxide (CO2) and total volatile organic component (TVOC). The monitored data are uploaded to the Internet server through the Long Range Wide Area Network (LoRaWAN) communication system. An intelligent algorithm has been developed to achieve an accuracy of 95% for the pedestrian count. There are a total of 74 sensor nodes that have been installed around Macquarie University and continued working for the last six months.
A New Asynchronous RTK Method to Mitigate Base Station Observation Outages
Authors: Du Yuan , Huang Guanwen , Zhang Qin , Gao Yang , Gao Yuting
Published: August 2019

Real-time kinematic (RTK) positioning is a satellite navigation technique that is widely used to enhance the precision of position data obtained from global navigation satellite systems (GNSS). This technique can reduce or eliminate significant correlation errors via the enhancement of the base station observation data. However, observations received by the base station are often interrupted, delayed, and/or discontinuous, and in the absence of base station observation data the corresponding positioning accuracy of a rover declines rapidly. With the strategies proposed till date, the positioning accuracy can only be maintained at the centimeter-level for a short span of time, no more than three min. To address this, a novel asynchronous RTK method (that addresses asynchronous errors) that can bridge significant gaps in the observations at the base station is proposed. First, satellite clock and orbital errors are eliminated using the products of the final precise ephemeris during post-processing or the ultra-rapid precise ephemeris during real-time processing. Then the tropospheric error is corrected using the Saastamoinen model and the asynchronous ionospheric delay is corrected using the carrier phase measurements from the rover receiver. Finally, a straightforward first-degree polynomial function is used to predict the residual asynchronous error. Experimental results demonstrate that the proposed approach can achieve centimeter-level accuracy for as long as 15 min during interruptions in both real-time and post-processing scenarios, and that the accuracy of the real-time scheme can be maintained for 15 min even when a large systematic error is projected in the U direction.
Single-Mode Tapered Vertical SU-8 Waveguide Fabricated by E-Beam Lithography for Analyte Sensing
Authors: Xin Yu , Pandraud Gregory , Zhang Yongmeng , French Paddy
Published: August 2019

In this paper, we propose a novel vertical SU-8 waveguide for evanescent analyte sensing. The waveguide is designed to possess a vertical and narrow structure to generate evanescent waves on both sides of the waveguide’s surface, aimed at increasing the sensitivity by enlarging the sensing areas. We performed simulations to monitor the influence of different parameters on the waveguide’s performance, including its height and width. E-beam lithography was used to fabricate the structure, as this one-step direct writing process enables easy, fast, and high-resolution fabrication. Furthermore, it reduces the sidewall roughness and decreases the induced scattering loss, which is a major source of waveguide loss. Couplers were added to improve the coupling efficiency and alignment tolerance, and will contribute to the feasibility of a plug-and-play optical system. Optical measurements show that the transmission loss is 1.03 ± 0.19 dB/cm. The absorption sensitivity was measured to be 4.8 dB per refractive index unit (dB/RIU) for saline solutions with various concentrations.
Evolution of Scintillation and Electrical Characteristics of AlGaN Double-Response Sensors During Proton Irradiation
Authors: Ceponis Tomas , Badokas Kazimieras , Deveikis Laimonas , Pavlov Jevgenij , Rumbauskas Vytautas , Kovalevskij Vitalij , Stanionyte Sandra , Tamulaitis Gintautas , Gaubas Eugenijus
Published: August 2019

Wide bandgap AlGaN is one of the most promising materials for the fabrication of radiation hard, double-response particle detectors for future collider facilities. However, the formation of defects during growth and fabrication of AlGaN-based devices is unavoidable. Furthermore, radiation defects are formed in detector structures during operation at extreme conditions. In this work, study of evolution of the proton-induced luminescence spectra and short-circuit current has been simultaneously performed during 1.6 MeV proton irradiation. GaN and AlGaN (with various Al concentrations) epi-layers grown by metalorganic chemical vapour deposition technique and Schottky diode structures have been examined. Variations of spectral and electrical parameters could be applied for the remote dosimetry of large hadron fluences.
The Synergy of Financial Volatility between China and the United States and the Risk Conduction Paths
Authors: Jiang Xiaochun , Sun Wei , Su Peng , Wang Ting
Published: August 2019

Based on monthly data of six major financial variables from January 1996 to December 2018, this paper employs a structural vector autoregressive model to synthesize financial conditions indices in China and the United States, investigates fluctuation characteristics and the synergy of financial volatility using a Markov regime switching model, and further analyzes the transmission paths of the financial risk by using threshold regression. The results show that there is an approximately three-year cycle in the financial fluctuations of both China and the United States, and such fluctuations have a distinct asymmetry. Two thresholds were applied (i.e., 0.361 and 0.583), taking the synergy index (SI) as the threshold variable. The impact of the trade factor is significant across all thresholds and is the basis of financial linkages. When the SI is less than 0.361, the exchange rate factor is the main cause of the financial cycle comovement change. As the financial volatility synergy increases, the asset factor and interest rate factor start to become the primary causes. When the level of synergy breaks through 0.583, the capital factor based on stock prices and house price is still the main path of financial market linkage and risk transmission, but the linkage of monetary policy shows a restraining effect on synergy. Therefore, it is necessary to monitor the financial cycle and pay attention to the coordination between countries in terms of policy regulation.
Zearalenone Contamination in Corn, Corn Products, and Swine Feed in China in 2016–2018 as Assessed by Magnetic Bead Immunoassay
Authors: Li Ming , Yang Chuqin , Mao Yuhao , Hong Xia , Du Daolin
Published: August 2019

In total, 405 samples of corn, corn products, and swine feed from China in 2016–2018 were surveyed for zearalenone (ZEN) contamination using a magnetic bead immunoassay-coupled biotin–streptavidin system (BAS-MBI). The developed BAS-MBI had a limit of detection (LOD) of 0.098 ng mL−1, with half-maximal inhibition concentration (IC50) of 0.71 ng mL−1 in working buffer, and an LOD of 0.98 ng g−1; the detection range was from 0.98 to 51.6 ng g−1 in authentic agricultural samples. The BAS-MBI has been demonstrated to be a powerful method for the rapid, sensitive, specific, and accurate determination of ZEN. The ZEN positivity rate reached the highest level of 40.6% in 133 samples in 2016; ZEN levels ranged from 1.8 to 1100.0 ng g−1, with an average level of 217.9 ng g−1. In 2017, the ZEN positivity rate was the lowest at 24.5% in 143 samples; ZEN levels ranged from 1.1 to 722.6 ng g−1, with an average of 166.7 ng g−1. In 2018, the ZEN positivity rate was 31.8% in 129 samples; ZEN levels ranged from 1.3 to 947.8 ng g−1, with an average of 157.0 ng g−1. About 20% of ZEN-positive samples exceeded maximum limit levels. An alternative method of ZEN detection and a valuable reference for ZEN contamination in corn and its related products in China are provided. This survey suggests the need for prevention of serious ZEN contamination, along with management for food safety and human health.
Migración de Amplatzer a arteria pulmonar: una complicación grave del cierre percutáneo en las comunicaciones interauriculares tipo ostium secundum
Authors: Solano Arce Jose , Gutiérrez  Alvarez Rafael , Méndez Zamora Ana M
Published: August 2019

En este artículo se presenta el caso de una paciente femenina de 11 añoscon una comunicación interauricular tipo ositum secundum quien, duranteel cierre percutáneo con dispositivo oclusivo tipo Amplatzer ®, sufre unacomplicación grave debido a la migración del mismo a la arteria pulmonar.Fue llevada a sala de operaciones de emergencia, donde se realizó unaextracción del dispositivo y reparación de la comunicación interauricular sincomplicaciones, con excelente evolución clínica.
Propuesta de proyectos que articulen la investigación y vinculación con la sociedad
Authors: Fernández Rodríguez Martha Maria
Published: August 2019

El objetivo de este artículo fue demostrar la integración de los procesos de investigación y vinculación con la sociedad, basado en  las experiencias en el Tecnológico Espíritu Santo (TES) en el proceso de fundamentación  de proyectos de Investigación y Vinculación con la Sociedad.  El problema científico que motivó la realización de esta investigación fue: ¿Cómo lograr la integración de investigación y vinculación con la sociedad en el Tecnológico Espíritu Santo? El contenido de la investigación formó parte de los resultados diagnóstico interno y externo que identifique el actuar de la institución. En el diagnóstico externo se tuvo en cuenta las demandas de la sociedad hacia la institución en las áreas que se corresponden con la oferta académica. Dichas demandas se ven en los ámbitos de la formación profesional a través de la docencia y la trasformación directa de la realidad social (entorno 2). Para ello se tuvo en cuenta:

* Vínculos históricos de la institución con entidades o espacios específicos.

* Ubicación de entidades con actividad afín a los perfiles de formación de la institución.

Para definir la trasformación directa de la realidad social (entorno 2) se realizó un diagnóstico en 2 cantones y un recinto que permitiera a la institución identificar de acuerdo a nuestro diagnóstico interno el mejor lugar de accionar para lograr la trasformación directa de la realidad social. Se emplearon métodos empíricos como la entrevista, la observación científica auxiliados de técnicas como la revisión de documentos y censo de población y vivienda. Se logró como resultado demostrar a través de 3 casos donde se realizó un diagnóstico concretar los proyectos de Vinculación con la Sociedad y de Investigación que guiarán los procesos sustantivos del TES en los próximos 5 años.
Experiencia de la Dirección de Ciencias Empresariales del Tecnológico Espíritu Santo (TES) en el desarrollo de competencias profesionales en función del emprendimiento
Authors: Tellez Anisleydi
Published: August 2019

La investigación se centró en el propósito de demostrar que el desarrollo de proyectos en cada una de las materias contribuye con la formación de competencias profesionales en función del emprendimiento. En tal sentido se buscó responde a la interrogante de ¿cómo contribuir con el desarrollo de competencias profesionales en función del emprendimiento?

Para el cumplimiento del objetivo de la investigación se utilizó una investigación descriptiva y analítica con un enfoque cualitativo y cuantitativo, mediante la evaluación de los proyectos presentados en la feria Business Week, y una encuesta realizada a los estudiantes que participaron en este evento. Se comprobó que el Aprendizaje Basado en Proyectos (ABP) contribuyó con el desarrollo de competencias esenciales para el emprendimiento, a través del desarrollo de capacidades de análisis, síntesis, resolución de problemas, creatividad, y adaptabilidad.
A Statistical Study of the Average Wages in the Republic of Belarus from a Gender-Focused Perspective
Authors: Kulak A. G.
Published: August 2019

The article presents an overview of the labour market in the Republic of Belarus, taking into account the gender factor. Indeed, the labour market of any state has a certain level of gender segregation and to reliably assess this phenomenon, first of all, it is necessary to develop a better understanding of the emerging patterns and trends in the dynamics of the core statistical indicators, and of what processes reduce or fuel gender asymmetry. The author carried out a statistical study on the wage levels of employed in the economy of the Republic of Belarus, and a comparative analysis of its average values for men and women. Despite the fact that the right to work and equal remuneration for men and women are enshrined in law, the problem of gender segregation in the labour market remains relevant. The effective solution of issues of gender inequality is impossible without taking into account the existing gender issues in the country and the main trends reflected in statistical indicators. The author applies statistical methodology of socio-economic analysis to show manifestations of gender inequality in its various forms, for example, hiring discrimination against women, gender pay gap, gender asymmetry in the distribution of workers between economic sectors and activities. Methods of variance analysis serve as a basis to study the relationship between the type of economic activity which employs Belarusian men and women and the level of average wages. Objective statistics suggests that the differences between men and women are often due not so much to biological factors as to social conditions (demands and development of culture, norms of society, etc.). Disclosing wage problems with statistical means, the author argues that gender equality acts not only as a goal but also as an essential means of achieving sustainable development, which is impossible without modernizing existing political and socio-economic mechanisms.
O medo no espaço urbano: contextos históricos e sociais
Authors: Rezende Bibiana Conceição
Published: August 2019

Diferentes sensações compõem e são percebidas no espaço, o medo é uma delas. Essa sensação pode estar presente nos mais diversos componentes do espaço público, desde sua arquitetura e estética, em um contexto histórico e até mesmo em um governo. Como contexto histórico o Brasil presenciou uma onda de medo que se instalou em muitos brasileiros durante as eleições de 2018. Apesar de envolver um aspecto geral da sociedade o medo será diferente de acordo com o contexto social de cada indivíduo, sendo aqueles que compõem as minorias sociais os que comumente se sentem mais inseguros no espaço urbano público.
A fragmentação socioespacial e sua relação com as dinâmicas afetivas
Authors: Stoian Victor Oliveira
Published: August 2019

Este artigo traz uma reflexão sobre processos envolvidos na produção contemporânea das cidades médias e a sua relação com a dinâmica afetiva dos sujeitos sociais autossegregados. Partindo de uma leitura de afeto baseada na obra do filosofo Baruch Spinoza, buscamos dialogar com outros autores que relacionam aspectos da vida subjetiva às práticas espaciais dos citadinos. O artigo está dividido em quatro partes. Na primeira parte, “Introdução”, apresentamos o tema ao leitor. Na segunda parte, trazemos o conceito de segregação e fragmentação socioespacial e sua relação com o medo. Na terceira parte, avançamos sobre a compreensão do medo como um dos principais afetos da vida contemporânea, sua relação com a autossegregação, a dinâmica afetiva correspondente e com uma certa sujeição que se submetem esses sujeitos sociais. Posteriormente fazemos uma reflexão de como os processos urbanos acima mencionados tem contribuído para uma homogeneização de aspectos das vida urbana que vão além do conteúdo material das cidades. E, por fim, apresentamos as considerações finais.
Cartografias alternativas: contribuições do programa de aquisição de alimentos materializadas no espaço das assentadas rurais
Authors: De Paula Larissa Araújo Coutinho
Published: August 2019

Almeja-se com este artigo, expor uma discussão teórica e resultados empíricos de experiência de pesquisa sobre o Programa de Aquisição de Alimentos (PAA) e suas consequências na vida de assentadas rurais associadas à Organização das Mulheres Unidas do Setor II da Gleba XV de Novembro (OMUS), no assentamento Gleba XV de Novembro em Rosana (SP), relacionando-os com a metodologia de cartografias alternativas. O uso de cartografias alternativas possibilitou incluir mais ativamente as mulheres participantes da pesquisa na realização do trabalho, além de evidenciar de modo mais efetivo as conquistas advindas desta política para suas vidas, ao longo dos anos de participação, no contexto de seus lotes e suas espacialidades.
Novos caminhos a partir de Frantz Fanon
Authors: Duarte Diego Elias Santana
Published: August 2019

O objetivo desta resenha crítica é demonstrar a importância do olhar de Frantz Fanon e do livro Pele negra, máscaras brancas para repensarmos pesquisas, pesquisadores, sujeitos, visões de mundo e auxiliar na construção de reflexões próximas aos sujeitos pesquisados, com maiores potências e autonomia epistêmica.
Analisis Mutasi pada Kodon 531 Pada Gen Rpob Mycobacterium tuberculosis Penyebab Resistensi Rifampisin
Authors: Cita Yatnita Parama , Putri Dwi Hilda
Published: August 2019

Tuberculosis (TB) is a serious disesase in the world. According to the WHO, it is estimated more than 3 million people die every year as a result of this infectious disease. One factor that causes diffi culty handling TB chemoteraphy is not effective against the bacteria Mycobacterium tuberculosis that causes TB . Effectiveness of treatment is often hampered by the emergence of bacterial resistance against M. Tuberculosis chemotherapy agents are given. From some research found that bacterial resistance may occur in more one type of chemotherapy agent also known as multi-drug resistance (MDR). Mycobacterium tuberculosis develop resistance mechanisms that are different from other bacteria in general. In prokaryotes, resistance is generally due to the transfer of genetic, either through plasmids,transposons and other. Reference sequence beta sub unit of RNAP protein M. Tuberculosis with accession number NP_215181.1 and M. tucerculosis rpoB gene with accession number NC_000962.3 used to obtain preliminary information from the data base www.ncbi.nlm.gov and www.uniprot.org . Mutation done according to several studies literature. Analysis of the composition, profi le, location and structure of protein using www.expasy.org, TMHMM and http://bioinf.cs.ucl.ac.uk/psipred. The primer design is done with Primer Design Program. Based on the analysis of mutation in the beta subunit of RNAP protein M. Tuberculosis, codon 531 (Ser ->Leu), it is known that mutations cause changes in some properties and structure of proteins. Possible changes affecting the nature of bacterial resistance to antibiotics rifampicin. However, further analysis needs to be done with the analysis of the docking technique.
The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol
Authors: Wibowo Much Ilham Novalisa Aji , Aeni Nur , Huda Zidna Mazayatul , Nurulita Nunuk Aries
Published: August 2019

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.
Formula Optimization of Fast Disintegrating Tablet (FDT) of Centella asiatica (L.) Urb. Ethanolic Extract
Authors: Lestari Agatha Budi Susiana , Fudholi Achmad , Nugroho Akhmad Kharis , Setyowati Erna Prawita
Published: August 2019

It has been proven that Centella asiatica (L.) Urb. herbs have an antioxidant activity that make it possible to used in preventing degenerative illness. Centella asiatica (L.) extract has been formulated to fast disintegrating tablet (FDT) dosage form. The aims of this study are to find the optimum formula of FDT of Centella asiatica (L.) Urb. The simplex lattice design method with 3 factors (which are mannitol as diluents, crospovidone as superdisintegrant and povidone as binder) and 2 levels of each was used. Centella asiatica (L.) Urb. extract was produce from maceration process using ethanol-water in ratio 75%:25% as a solvent. Direct compress method was used to produce the FDT. The results show that the optimum composition between excipients FDT formula could be reached, which fullfiled the physical properties parameter which are the hardness, friability and disintegration time of FDT.
Clinical Trial the Efficacy of Boiled Hypertension Herbs Compared with Steeped Hypertension Herbs
Authors: Triyono Agus , Ridha Peristiwan , Ardianto Danang
Published: August 2019

To search for scientific evidence the efficacy of herbal medicine, has conducted clinical trial the efficacy of  hypertension herbs compared with hydrochlorotiazide. Efficacy of clinical trials were conducted with the design open-label, randomized clinical trials and parallel design. It was involving 80 subjects who have met the inclusion and exclusion criteria. Subjects randomized study that is divided into two groups, a herbs group  and hydrochlorotiazide group. Subjects performed once a week to check the complaint history, the development of clinical symptoms, the possibility of side effects and diagnostic physical examination. Assessment score of quality of life by questionnaires Short Form 36 (SF-36) every four weeks. Results showed that administration of herbal medicine for 56 days hypertension, lower blood pressure (systolic and diastolikic) is equivalent to hydrochlorotiazide (HCT). Lowering blood pressure to normal (normotensive) 62.7% of the subjects. Eliminating the clinical symptoms of hypertension (dizziness / headache, stiff neck / whiny and myalgia) herbs group subjects at almost the same time with the  hidrochlorthiazide group. Raising the quality of life scores (SF-36) subjects herbs group equivalent to the increase in quality of life scores (SF-36)  hydrochlorotiazide group.
Analysis of SGPT and SGOT on Rats Induced By Isoniazid to Determine Dose and Hepatoprotective Characteristic of Unripe Pineapple Juice (Ananas comosus L. Merr)
Authors: Rahayu Lestari , Yantih Novi , Supomo Yoana
Published: August 2019

As a consequence of Isoniazid (INH) consumption in longterm is the damage on the liver cells which is detected in high level of SGPT and SGOT in blood. Unripe pineapple fruits contained higher level of phenolic acid and flavonoids compounds than the ripe pineapple fruits, and as a result the unripe pineapple fruits juice has higher hepatoprotective potency  than ripe pineapple fruits juice. The hepatoprotective activity of  unripe pineapple fruits juice was determined by measuring the SGPT and SGOT level on rat blood. There were prepared 24 rats, divided into 6 groups, i.e. normal control group, positive control group, negative control group, and 3 test goups that were induced  by INH and each was given the unripe pineapple fruits juice at 1 hour before and 1 hour after INH induction, with the dose of 2 mL and 4 mL. Treatment was conducted in 14 days, consecutively. To each of the rat groups, blood was taken out through the eye orbital sinus, i.e. on the first day, on the hour-0 and hour-3, and then the SGPT and SGOT level were measured. Later on, blood were taken out on the day-4, day-7, and day-14. On the rat blood that were taken out, were analyzed descriptively and statistically to determined the optimum dose and the time of the unripe pineapple fruits juice feeding in related with a hepatoprotective potency. Result showed that feeding of the unripe pineapple fruit juice 1 hour after INH induction with the dose of 2 mL during 14 days has the best hepatoprotective activity.
Subacute Toxicity Effect of Psidium guajava Leaves Ethanolic Extract on Gaster in Wistar Rat
Authors: Utami Esti Dyah , Baroroh Hanif Nasiatul , Nuryanti Nuryanti
Published: August 2019

Psidium guajava leaves have been proven be antiallergic. From the results of previous research, the ethanol extract of leaves is included in the class materials "Practically Non-Toxic." However, repeated administration may cause toxic effects on body organs, one of which was gaster. Subchronic toxicity had been carried out on 20 male Wistar rats and 20 female wistar albino rats. The test materials were administered orally, every day for 28 days. There are 3 (three) doses of test materials which were 250 mg, 500 mg, and 750 mg/kg BW. Observation of the general toxic effect was performed everyday, then organ sampling was performed on day 29 to examined gaster histology and score using Barthel-Manja methods. The result showed that administration of Psidium guajava leaves extract for 28 days caused physical and behavioral changes, caused soft and mucous feces, and also respiratory disorder. But the extract at dose 250 mg, 500 mg and 750 mg/kg BW did not caused changes on gaster histology profile and score. In conclusion, the extract did not have subchronic toxicity effect on gaster rat, but might caused general toxic effect in the higher dose.
Effect of Egg White Gel againts Burn Healing on White Rat (Rattus novergicus)
Authors: Hendriati Lucia , Hamid Iwan Sahrial , Widodo Teguh , Wandasari Chintya , Risata Putu Mirah
Published: August 2019

Egg whites traditionally used for the treatment of burns. In this research, egg white was formulated as gel dosage form for easy to use and covered unpleasant odor. The aim of the study was to determined effectivity of egg white in gel dosage form against the healing of superficial partial thickness burns (second degree) on white rats (Rattus novergicus) Wistar strain with parameters were macrophages, fibroblasts, collagen deposit density and wound healing. Research subject were  male white rats aged 3 months. Second degree burns on the back of rat with a diameter of 2 cm were made by  using a hot coin with temperature 80 ° C for 20 seconds. 18 white rats divided into 3 groups of treatment that is negative control using 0.9% NaCl, positive control using Bioplacenton®, and 40% egg white gel as treatment. Based on the experimental results, white egg gel  given topically decreased the number of macrophages and increase the number of fibroblasts, increase the density of collagen deposits and speed up wound healing on second degree burns. White egg gel had significantly difference with negative control of NaCl 0.9% and no significantly difference  with Bioplasenton as positive control.
Uji Efektifitas Ekstras Etanol Buah Naga Putih (Hylocereus undatus) Terhadap Penurunan Kadar Asam Urat Darah Pada Mencit (Mus musculus)
Authors: Amir Mellova , Purukan Juliana Irem Adriana
Published: August 2019

White dragon fruit (Hylocereus undatus) contains flavonoids that can inhibit the action of xanthine oxidase enzymes that can reduce uric acid levels. The aim of this research is to know the effectiveness of white dragon ethanol extract in decreasing uric acid level in caffeine-induced mice with dose 0.39 mg/25 g BW (body weight). White dragon fruit samples taken at the age of 50-55 days after the flower appeared. In the effectiveness test, animals were divided into five groups of treatments and induced by caffeine i.e. negatively control group induced only by caffeine, positive control group given allopurinol 0.26 mg/25 g BW, group of dose 0.455 mg/25 g BW, group of dose 0.91 mg/25 g BW, group of dose 1.82 mg/25 g BW ethanol extract of white dragon fruit. Measurements of blood uric acid levels were performed before induction (day 1), after induction or before administration of the extract (day 6) and after administration of the extract on (days 9, 12 and 15). The results showed that ethanol extract of white dragon fruit dose 1.82 mg/25 g BW could significantly lower blood uric acid levels in mice compared to dose 0.455 mg and dose 0.91 mg/25 g BW, but not significantly different with positive control (allopurinol) with percentage decrease of 92.16%.

Keywords: White dragon fruit extract, blood uric acid, caffeine, allopurinol.
PENGARUH ELECTRONIC WORD OF MOUTH DI MEDIA SOSIAL INSTAGRAM DAN ATRIBUT PRODUK WISATA TERHADAP KEPUTUSAN BERKUNJUNG DI FLOATING MARKET LEMBANG
Authors: Muflikhah, Vegita , P. Mbulu Yustisia , Adhi Gunadi I Made
Published: August 2019

Abstract Electronic word of mouth becomes an important place for the visitors to give their opinion about their experience, because when someone is doing electronic word of mouth, they will pay attention to the aspects of the tourism products that have became the experience in visiting a tourism object. This research attempts to identify electronic word of mouth on Instagram Floating Market Lembang’s social media, identify tourism product attributes in Floating Market Lembang, analyze influence of electronic word of mouth on Instagram social media and tourism product attributes towards tourist decision to visit Floating Market Lembang. The research design used is quantitative with multiple linear regression data analysis techniques. The results of this research have identified electronic word of mouth on Instagram social media and tourism product attributes in Floating Market Lembang, it was found that three electronic word of mouth variables consisting of intensity, valence of opinion and content significantly influence the decision variables visited at the Floating Market Lembang. While tourism product attributes found only one of three variables that significantly influence the decision variables visited in the Floating Market Lembang. One of these variables is tourism attraction, while the other two variables that do not influence the decision variables visited in Floating Market Lembang are amenities and accessibility.
Perencanaan Program Interpretasi Untuk Mendukung Kegiatan Ekowisata Di Citamiang Bogor
Authors: Weullas Widya , Rusli Meizar , K. Kausar Devi Roza
Published: August 2019

Penelitian ini bertujuan mengukur tingkat kepentingan daya tarik ekowisata Citamiang, Jawa Barat, bagi wisatawan dan juga kepercayaan wisatawan terhadap daya tarik tersebut. Sesuai dari hasil analisis Fishbein bahwa atraksi yang sangat dipercaya sebagai daya tarik ekowisata adalah atraksi trek herbal yakni dengan skor 2.04. Trek herbal memiliki point edukasi yang penting untuk wisatawan yang berkunjung ke Citamiang. Sedangkan sikap konsumen terbesar ada di atribut Jalan, yakni sebesar 10,43. Infrastruktur yang ada di Citamiang memang sudah dianggap baik oleh wisatawan. Dari hasil tersebut, dapat dibuat program interpretasi verbal dan non verbal yang sesuai untuk daya tarik ekowisata ini yaitu ‘Meet The Herbs’ sebagai program verbal dan ‘Find More Indonesia’ sebagai program non verbal. Sasarannya adala remaja-dewasa, karena karakteristik wisatawan yang datang ke Citamiang didominasi oleh remaja-dewasa. Program tersebut ditujukan agar wisatawan dapat menikmati dan sekaligus mendapatkan edukasi lingkungan.