bluuebunny/crossref_metadata_2025 huggingface dataset:
Cell-free DNA From Pleural Effusion Samples: Is It Right for Molecular Testing in Lung Adenocarcinoma?
Authors: Mokánszki Attila , Bádon Emese Sarolta , Mónus Anikó , Tóth László , Bittner Nóra , Méhes Gábor
Published: March 2021

Pathogenic molecular features gained specific significance in therapeutic decisions in lung carcinoma in the past decade. Initial and follow up genetic testing requres appropriate amounts and quality of tumor derived DNA, but tumor sampling, especially for disease monitoring is generally limited. Further to the peripheral blood (PB), samples from pleural fluid, accumulating in diverse lung processes might serve as an alternative source for cell-free DNA (cfDNA) for genetic profiling. In our study, cfDNA isolated from the pleural effusion and from the PB, and genomic DNA (gDNA) obtained from tissue/cellular samples were analyzed and compared from altogether 65 patients with pulmonary disease, including 36 lung adenocarcinomas. The quantity of effusion cfDNA yield appeared to be significantly higher compared to that from simultaneously collected PB plasma (23.2 vs. 4.8 ng/μl, p < 0.05). Gene mutations could be safely demonstrated from the effusion cfDNA fraction obtained from adenocarcinoma patients, 3/36 EGFR, 9/36 KRAS and 1/36 BRAF gene variants were detected. In this series, 9/13 samples showed an effusion+/plasma-mutational status, while only 1/13 samples presented with the opposite findings (effusion-/plasma+). gDNA analysis from sediment cell blocks from the identical effusion sample was surprisingly ineffective for lung adenocarcinoma profiling due to the low DNA yield. In conclusion, the cell free supernatant of pleural effusions appears to concentrate cancer derived cfDNA and seems to be particularly suitable for serial genotyping of pulmonary adenocarcinoma.
An Overview on the Use of Near Infrared Spectroscopy (NIRS) on Farms for the Management of Dairy Cows
Authors: Evangelista Chiara , Basiricò Loredana , Bernabucci Umberto
Published: March 2021

Dairy farming is increasingly affected by the digital revolution. To respond to current challenges—such as environmental, economic, and social sustainability—new technologies must be adopted, entering the perspective of precision livestock farming. This is made possible by the development of countless sensors to be adopted in the barn. The technology that is affecting various aspects of dairy cattle breeding is certainly near infrared spectroscopy (NIRS) which is versatile and can be used online/inline to evaluate and control the critical points of the production process by entering the PAT (process analytical technology). In the barn, NIRS currently can obtain information on the chemical-physical composition of raw materials, total mixed ration (TMR), feces and digestibility, chemical and technological analysis of milk. All this in a short time by eliminating the waiting times for analysis response and costs, allowing an improvement of livestock management. Many studies affirm the validity of NIRS as a reliable and predictive technology against multiple relevant parameters in matrices such as raw feed, TMR, feces, and milk. This review highlights the usefulness of NIRS technology in dairy farm with particular attention to portable instrumentation usable directly on the farm.
Dietary Effects of Extra Virgin Olive Oil Extracted by Ultrasound Technology or Refined Olive Oil on the Quality Traits of Pork and “Capocollo di Martina Franca” Dry-Cured Meat
Authors: Colonna Maria Antonietta , Tarricone Simona , Giannico Francesco , Selvaggi Maria , Carriero Francesco , Crupi Pasquale , Corbo Filomena , Clodoveo Maria Lisa
Published: March 2021

The “Capocollo di Martina Franca” is a traditional dry-cured pig meat product made in Apulia. The dietary fat source is able to influence the lipid profile of muscle and subcutaneous fat in pork, thus affecting its nutritional and sensorial quality as well as its suitability for technological processing. The aim of the study was to evaluate the effect of a diet containing extra virgin olive oil (EVOO, 3%, as-fed basis) extracted by ultrasound technology in comparison to refined olive oil (ROO, 3%, as-fed basis) on the quality of pig meat (longissimus lumborum muscle) and capocollo in relation to its storage time after seasoning (t1 = 0 vs. t2 = +6 months). The EVOO diet lowered the concentration of myristic, palmitic, stearic and total saturated fatty acids (SFA) and increased oleic, linoleic, total monounsaturated (MUFAs), polyunsaturated (PUFAs) and n-3 and n-6 fatty acids in pig meat; moreover, the atherogenicity and thrombogenicity indices were lowered, with potential benefits for human health. The overall quality of capocollo was positively affected by the EVOO diet, although storage for 6 months after ripening levelled the protective effects of extra virgin olive oil in comparison with refined olive oil.
Growth Performance, Carcass Characteristics, and Blood Metabolites of Lambs Supplemented with a Polyherbal Mixture
Authors: Orzuna-Orzuna José Felipe , Dorantes-Iturbide Griselda , Lara-Bueno Alejandro , Mendoza-Martínez Germán David , Miranda-Romero Luis Alberto , Hernández-García Pedro Abel
Published: March 2021

The objective of this study was to determine the effects of the supplementation of a polyherbal mixture (HM) on the productive performance, carcass characteristics, meat quality, and the profile of blood metabolites of lambs fed a high-concentrate diet. Thirty-six male Pelibuey lambs (25.21 ± 0.96 kg BW) were housed in individual pens during a 56-day feeding period and were randomly assigned to four treatments: (1) Control (CON): Basal diet without HM; (2) HM1: CON + 1 g of HM kg−1 dry matter (DM); (3) HM2: CON + 2 g of HM kg−1 DM; and (4) HM3: CON + 3 g of HM kg−1 DM. Data were analyzed using the GLM (General Linear Model) procedure of statistical analysis system (SAS), and linear and quadratic effects were tested to evaluate the effects of the HM level. A quadratic increase was observed in the dry matter intake and in daily weight gain (p < 0.05) of lambs fed with HM2 and HM1, respectively. However, final body weight, body condition, carcass characteristics, and meat quality were similar among treatments (p > 0.05). It was observed a linear increase (p < 0.05) in the mean corpuscular hemoglobin concentration. Lymphocytes in blood from lambs supplemented with the HM1 diet increased and segmented neutrophils decreased compared to lambs receiving the CON treatment (p < 0.05). The concentration of uric acid in the blood had a linear increase (p < 0.05) and the serum creatinine level decreased (p < 0.05) as the HM dietary dose increased. In conclusion, dietary inclusion of 2 and 1 g of HM kg−1 of DM improves feed consumption and daily weight gain, respectively, without affecting carcass characteristics, meat quality, and health status on finishing lambs.
Comparative Growth and Economic Performances between Indigenous Swamp and Murrah Crossbred Buffaloes in Malaysia
Authors: Mohd Azmi Amirul Faiz , Abu Hassim Hasliza , Mohd Nor Norhariani , Ahmad Hafandi , Meng Goh Yong , Abdullah Punimin , Abu Bakar Md Zuki , Vera Jaizurah , Mohd Deli Nurain Syahida , Salleh Annas , Zamri-Saad Mohd
Published: March 2021

This study was conducted to compare the growth and economic performances between Swamp and Murrah crossbred buffaloes. The records of 108 Swamp and 276 Murrah crossbred buffaloes born between January 2010 and December 2015 were used in this study. The farm was practicing an extensive grazing system without supplementation from January 2010 to December 2011 (pre-intervention) and a new implementation of supplement in the feeding regime from January 2012 to December 2015 (post-intervention). The birth, weaning, and body weight at three monthly intervals, number of calves born, and mortality rate of calves at different years and during pre- and post-intervention were analyzed using a general linear model procedure. The interventions in 2012 had a positive effect on increasing the number of calves born for both breeds, average birth weight, economic performance, and reduce mortality calf rate. As a result, the birth weight of Murrah crossbred buffaloes was higher (36.63 ± 0.50 kg) than Swamp buffaloes (34.69 ± 0.40 kg) (p < 0.05). The average pre-weaning daily weight gain for Swamp and Murrah crossbred buffaloes was 0.73 and 0.98 kg/day (p < 0.05), while the average post-weaning daily weight gain was 0.39 and 0.44 kg/day, respectively (p < 0.05). The Swamp and Murrah crossbred buffaloes achieved the targeted market weight of 250 kg at 18 and 15 months old, respectively, while the targeted breeding weight of 385 kg was achieved at 30 and 26 months old, respectively. In this farm, on average a total of 64 calves were born yearly, with the ratio number of born calves per number of mated dams recorded higher in Murrah crossbred buffaloes as compared to Swamp buffalo (0.64 vs. 0.37) (p < 0.05). Furthermore, the average number of calves born in the post-intervention period (January 2012–December 2015) was significantly higher than in the pre-intervention period (January 2010–December 2011), respectively (Swamp: 23 vs. 8 and Murrah crossbred: 53 vs. 31, respectively) (p < 0.05). Partial budget method was used to estimate the net gain or loss between the two breeds. The average annual revenue was 2304.14 MYR (566.13 USD) for Swamp buffaloes and 4531.50 MYR (1113.39 USD) for Murrah crossbred buffaloes. The average annual cost saving was 340.02 MYR (83.54 USD) for Swamp and 215.75 MYR (53.01 USD) for Murrah crossbred buffaloes. On the other hand, annual added cost was 84.95 MYR (20.87 USD) for Swamp and 96.76 MYR (23.77 USD) for Murrah crossbred buffaloes. Therefore, the annual net benefit was 2559.21 MYR (628.80 USD) for Swamp and 4650.49 MYR (1142.63 USD) for Murrah crossbred buffaloes. As a conclusion, this study had shown that the higher average daily weight gain contributes to better cost savings, as shown by the crossbred buffaloes.
Mechanical Effect of Performance Pressure Boots on Cadaveric Equine Hindlimb Fetlock Biomechanics
Authors: Symons Jennifer
Published: March 2021

Pressure boots are applied to hind limbs of showjumping horses with the intent to enhance jumping form. Manufacturers claim acupressure points enhance proprioception of hind limbs. With this increased awareness, horses are expected to retract their hind limbs to clear jump rails. This research aimed to investigate a more direct, mechanical effect of pressure boots on hind limb biomechanics. Cadaveric hind limbs (n = 6) were mechanically loaded in axial compression (3 cycles at 0.25 Hz, displacement control ~3300 N) with (2 trials) and without (2 trials) a pressure boot applied. During mechanical loading, fetlock angle was measured using bone fixed pins with retroreflective markers (30 Hz). Changes in limb load and fetlock angle between unloaded and loaded states, as well as average fetlock joint stiffness, were compared between trials with and without the pressure boot via ANOVA. Differences in measured loads between trials with and without the boot were observed in both unloaded (Δ = 6 N, p = 0.05) and loaded states (Δ = 25 N, p = 0.002). Trials with the boot had greater average fetlock stiffness (Δ = 3 N/degree, p = 0.001). Differences in loads with and without boots may increase with greater fetlock angles when cantering and jumping. These mechanical effects of pressure boots may contribute to greater tensile loading of palmar tendons and ligaments, and likelihood of musculoskeletal injury that can be related to animal welfare issues.
The Effect of Replacing Genetically Modified Soybean Meal with 00-Rapeseed Meal, Faba Bean and Yellow Lupine in Grower-Finisher Diets on Nutrient Digestibility, Nitrogen Retention, Selected Blood Biochemical Parameters and Fattening Performance of Pigs
Authors: Sobotka Wiesław , Fiedorowicz-Szatkowska Elwira
Published: March 2021

The aim of this study was to determine the effect of partial and total replacement of protein from genetically modified soybean meal (GM-SBM) with protein from 00-rapeseed meal (00-RSM), alone or in combination with protein from low-tannin faba bean (Vicia faba L.) seeds (FB) or low-alkaloid yellow lupine (Lupinus luteus L.) seeds (YL) in grower-finisher diets on nutrient digestibility, nitrogen retention and utilization, selected blood biochemical parameters, fattening performance of pigs and carcass quality traits. Two digestibility-balance trials and one feeding trial were performed during two-phase fattening on male hybrid Danbred growing-finishing pigs were divided into four groups. The pigs were fed grower diets where 50% of GM-SBM protein (diet S-c) was replaced with 00-RSM protein (diet R), 00-RSM and FB protein (diet R + FB) or 00-RSM and YL protein (diet R + YL), and finisher diets where 100% of GM-SBM protein (diet S-c) was totally replaced with 00-RSM protein (diet R), and with 00-RSM and FB protein (diet R + FB) or YL protein (diet R + YL) in 50%. It was found that partial (50% in grower diets) and total (100% in finisher diets) replacement of GM-SBM protein with 00-RSM protein combined with FB or YL protein had no adverse effect on nutrient and energy digestibility, N balance, serum of blood carbohydrate and protein metabolism or the biochemical parameters of liver and kidney function. Protein from 00-RSM (6%) and FB seeds (10%/12%) contributed to high daily gains and high feed conversion efficiency. Protein from 00-RSM (6%) and YL seeds (6%/7%) in grower-finisher diets led to a further improvement in fattening performance. The analyzed vegetable protein sources had no negative influence on carcass quality. The results of the present study indicate that 00-RSM protein combined with protein from low-tannin FB or low-alkaloid YL seeds can be valuable high-protein feed ingredients alternative to GM-SBM in pig nutrition.
Effect of Dietary Hazelnut Peels on the Contents of Fatty Acids, Cholesterol, Tocopherols, and on the Shelf-Life of Ripened Ewe Cheese
Authors: Marino Vita Maria , Rapisarda Teresa , Caccamo Margherita , Valenti Bernardo , Priolo Alessandro , Luciano Giuseppe , Natalello Antonio , Campione Adriana , Pauselli Mariano
Published: March 2021

Hazelnut peel (HNP), a by-product from the chocolate industry, is considered to be a suitable ingredient to be included in the diet of ruminants. This study aimed to evaluate the effect of feeding dairy ewes with a diet containing HNP on ripened cheese quality, including fatty acid (FA) profile, cholesterol, and tocopherol content, as well as stability during storage under commercial conditions. In total, 10 experimental cheeses were produced with bulk milk obtained from ewes fed a commercial concentrate (C group; n = 5) or a concentrate containing 36% HNP in dry matter (HNP group; n = 5). After 40 days of aging, each cheese was sub-sampled into three slices: one was analyzed immediately (C0 and HNP0), and the other two were refrigerated and analyzed after seven days (C7 and HNP7) and 14 days (C14 and HNP14), respectively. Compared to C, HNP cheese had more than twice as many tocopherols and mono-unsaturated FA and respectively 38% and 24% less of cholesterol and saturated FA. Tocopherols and cholesterol levels remained rather stable up to 14 days of storage regardless of the experimental group, suggesting no cholesterol oxidation. Therefore, the inclusion of HNP in ewe diets could be a valid resource to produce cheese with a healthier lipid profile and higher tocopherols content.
A Flavonoid-Rich Extract of Mandarin Juice Counteracts 6-OHDA-Induced Oxidative Stress in SH-SY5Y Cells and Modulates Parkinson-Related Genes
Authors: Cirmi Santa , Maugeri Alessandro , Lombardo Giovanni Enrico , Russo Caterina , Musumeci Laura , Gangemi Sebastiano , Calapai Gioacchino , Barreca Davide , Navarra Michele
Published: March 2021

Parkinson’s disease (PD) is a degenerative disorder of the nervous system due to unceasing impairment of dopaminergic neurons situated in the substantia nigra. At present, anti-PD drugs acting on dopamine receptors are mainly symptomatic and have only very limited neuroprotective effects, whereas drugs slowing down neurodegeneration of dopaminergic neurons and deterioration of clinical symptoms are not yet available. Given that, the development of more valuable pharmacological strategies is highly demanded. Comprehensive research on innovative neuroprotective drugs has proven that anti-inflammatory and antioxidant molecules from food sources may prevent and/or counteract neurodegenerative diseases, such as PD. The present study was aimed at the evaluation the protective effect of mandarin juice extract (MJe) against 6-hydroxydopamine (6-OHDA)-induced SH-SY5Y human neuroblastoma cell death. Treatment of differentiated SH-SY5Y cells with 6-OHDA brought cell death, and specifically, apoptosis, which was significantly inhibited by the preincubation with MJe through caspase 3 blockage and the modulation of p53, Bax, and Bcl-2 genes. In addition, it showed antioxidant properties in abiotic models as well as in vitro, where it reduced both reactive oxygen and nitrogen species induced by 6-OHDA, along with restored mitochondrial membrane potential, and prevented the oxidative DNA damage evoked by 6-OHDA. Furthermore, MJe restored the impaired balance of SNCA, LRRK2, PINK1, parkin, and DJ-1 gene levels, PD-related factors, caused by 6-OHDA oxidative stress. Overall, these results indicate that MJe exerts neuroprotective effects against 6-OHDA-induced cell death in SH-SY5Y cells by mechanisms involving both the specific interaction with intracellular pathways and its antioxidant capability. Our study suggests a novel possible strategy to prevent and/or ameliorate neurodegenerative diseases, such as PD.
Total Analysis of the Major Secoiridoids in Extra Virgin Olive Oil: Validation of an UHPLC-ESI-MS/MS Method
Authors: Lozano-Castellón Julián , López-Yerena Anallely , Olmo-Cunillera Alexandra , Jáuregui Olga , Pérez Maria , Lamuela-Raventós Rosa Mª , Vallverdú-Queralt Anna
Published: March 2021

Extra virgin olive oil (EVOO), one of the key foods of the Mediterranean diet, is distinguished by its high content of nutritional and antioxidant compounds compared to other vegetable oils. During EVOO production, the major secoiridoids of EVOO, oleacein, oleocanthal, ligstroside, and oleuropein aglycones, undergo a series of transformations to open- and closed-structure forms. The resulting mixture of compounds can become more complex during the analytical procedure, due to the keto-enol tautomerism of the open forms and their interaction with polar solvents, and therefore more challenging to analyze. Employing the same extraction method used to analyze the other EVOO phenolic compounds, we report here a simple UHPLC-ESI-MS/MS procedure for the quantification of those secoiridoids that is able to co-elute the different isomers of each compound. The method was validated following AOAC guidelines, and the matrix effect and recoveries were within satisfactory limits.
“Here Are the Rules: Ignore All Rules”: Automatic Contradiction Detection in Spanish
Authors: Sepúlveda-Torres Robiert , Bonet-Jover Alba , Saquete Estela
Published: March 2021

This paper tackles automatic detection of contradictions in Spanish within the news domain. Two pieces of information are classified as compatible, contradictory, or unrelated information. To deal with the task, the ES-Contradiction dataset was created. This dataset contains a balanced number of each of the three types of information. The novelty of the research is the fine-grained annotation of the different types of contradictions in the dataset. Presently, four different types of contradictions are covered in the contradiction examples: negation, antonyms, numerical, and structural. However, future work will extend the dataset with all possible types of contradictions. In order to validate the effectiveness of the dataset, a pretrained model is used (BETO), and after performing different experiments, the system is able to detect contradiction with a F1m of 92.47%. Regarding the type of contradictions, the best results are obtained with negation contradiction (F1m = 98%), whereas structural contradictions obtain the lowest results (F1m = 69%) because of the smaller number of structural examples, due to the complexity of generating them. When dealing with a more generalistic dataset such as XNLI, our dataset fails to detect most of the contradictions properly, as the size of both datasets are very different and our dataset only covers four types of contradiction. However, using the classification of the contradictions leads us to conclude that there are highly complex contradictions that will need external knowledge in order to be properly detected and this will avoid the need for them to be previously exposed to the system.
A New Real-Time Detection and Tracking Method in Videos for Small Target Traffic Signs
Authors: Song Shaojian , Li Yuanchao , Huang Qingbao , Li Gang
Published: March 2021

It is a challenging task for self-driving vehicles in Real-World traffic scenarios to find a trade-off between the real-time performance and the high accuracy of the detection, recognition, and tracking in videos. This issue is addressed in this paper with an improved YOLOv3 (You Only Look Once) and a multi-object tracking algorithm (Deep-Sort). First, data augmentation is employed for small sample traffic signs to address the problem of an extremely unbalanced distribution of different samples in the dataset. Second, a new architecture of YOLOv3 is proposed to make it more suitable for detecting small targets. The detailed method is (1) removing the output feature map corresponding to the 32-times subsampling of the input image in the original YOLOv3 structure to reduce its computational costs and improve its real-time performances; (2) adding an output feature map of 4-times subsampling to improve its detection capability for the small traffic signs; (3) Deep-Sort is integrated into the detection method to improve the precision and robustness of multi-object detection, and the tracking ability in videos. Finally, our method demonstrated better detection capabilities, with respect to state-of-the-art approaches, which precision, recall and mAP is 91%, 90%, and 84.76% respectively.
Effects of Water-to-Cement Ratio on Pore Structure Evolution and Strength Development of Cement Slurry Based on HYMOSTRUC3D and Micro-CT
Authors: Zheng Shaojun , Liu Tianle , Jiang Guosheng , Fang Changliang , Qu Bo , Gao Peng , Li Lixia , Feng Yingtao
Published: March 2021

Changing the water-to-cement ratio is one of the major ways to develop cement slurry with different densities, which in turn will greatly affect the pore structure and mechanical properties of cement slurry. In the current study, the cement hydration model HYMOSTRUC3D was used to investigate the effects of water-to-cement (w/c) ratio (0.40, 0.44, 0.50) on the pore structure evolution and strength development of cement slurry. The microstructure of the cement stone was characterized via scanning electron microscope (SEM) and micro-computed tomography (micro-CT), and the mechanical strength of the cement stone was tested and analyzed via a mechanical tester. The simulated compressive strength and capillary porosity are in good agreement with the measured data, where the relative error between the simulated results and measured results are within 0.6~10.7% and 13.04~25.31%, respectively. The capillary porosity is proved as the main factor affecting the compressive strength of cement stone with different w/c ratios. Herein, the mathematical relationship between the measured capillary porosity and compressive strength could be well fitted via the mathematical prediction models of the Balshin function (R2 = 0.95), Ryshkewitch function (R2 = 0.94), Schiller function (R2 = 0.96), and the linear regression function (R2 = 0.95). Moreover, the linear regression function (y = −2.38x + 82.76) can be used to characterize and predict the quantitative relationship between the compressive strength and capillary porosity of cement stone. The findings in this study will provide a reference value in the fields of oil and gas cementing and building concrete.
International Airline Alliance Network Design with Uncertainty
Authors: Yang Wendong , Shao Jiajia , Jiang Yun , Xu Zhengjia , Tsourdos Antonios
Published: March 2021

This paper addresses the alliance route network design problem considering uncertainty in the unit transportation cost. An alliance route network was constructed based on a hub-and-spoke (HS) network, in which airlines could achieve inter-area passenger transport through their international gateways. The design problem was formulated with a robust model containing a set of uncertain cost parameters. The model was established based on a three-subscript model of an HS network. A case study with real-world data was used to test the proposed model. The results showed that this robust solution can reduce the impact of cost uncertainty.
Robust Engineering for the Design of Resilient Manufacturing Systems
Authors: Mourtzis Dimitris , Angelopoulos John , Panopoulos Nikos
Published: March 2021

As the industrial requirements change rapidly due to the drastic evolution of technology, the necessity of quickly investigating potential system alternatives towards a more efficient manufacturing system design arises more intensely than ever. Production system simulation has proven to be a powerful tool for designing and evaluating a manufacturing system due to its low cost, quick analysis, low risk and meaningful insight that it may provide, improving the understanding of the influence of each component. In this research work, the design and evaluation of a real manufacturing system using Discrete Event Simulation (DES), based on real data obtained from the copper industry is presented. The current production system is modelled, and the real production data are analyzed and connected. The impact identification of the individual parameters on the response of the system is accomplished towards the selection of the proper configurations for near-optimum outcome. Further to that, different simulation scenarios based on the Design of Experiments (DOE) are studied towards the optimization of the production, under predefined product analogies.
New Approach in Human-AI Interaction by Reinforcement-Imitation Learning
Authors: Navidi Neda , Landry Rene
Published: March 2021

Reinforcement Learning (RL) provides effective results with an agent learning from a stand-alone reward function. However, it presents unique challenges with large amounts of environment states and action spaces, as well as in the determination of rewards. Imitation Learning (IL) offers a promising solution for those challenges using a teacher. In IL, the learning process can take advantage of human-sourced assistance and/or control over the agent and environment. A human teacher and an agent learner are considered in this study. The teacher takes part in the agent’s training towards dealing with the environment, tackling a specific objective, and achieving a predefined goal. This paper proposes a novel approach combining IL with different types of RL methods, namely, state-action-reward-state-action (SARSA) and Asynchronous Advantage Actor–Critic Agents (A3C), to overcome the problems of both stand-alone systems. How to effectively leverage the teacher’s feedback—be it direct binary or indirect detailed—for the agent learner to learn sequential decision-making policies is addressed. The results of this study on various OpenAI-Gym environments show that this algorithmic method can be incorporated with different combinations, and significantly decreases both human endeavors and tedious exploration process.
Using Mixed Reality (MR) to Improve On-Site Design Experience in Community Planning
Authors: Dan Yuze , Shen Zhenjiang , Zhu Yiyun , Huang Ling
Published: March 2021

In recent years, designing in existing environments has been consistently emphasized in community planning. However, practicing such on-site design is not easy for designers, because the current technical conditions do not allow virtual design objects into real environments for 3D visualization and interaction. Thus, designers’ intuitive design perceptions, accurate design judgments, and convenient design decisions are hardly supported. This paper explores the possibilities of using mixed reality (MR) technology to improve designers’ on-site design experiences in community planning. For this, we introduced an MR design support system (MR-DSS) for the interactive on-site 3D visualization of virtual design objects. With the MR-DSS, we performed a design experiment with sixteen participants in a typical on-site design scene of community planning. The results showed that the MR technology could provide designers with intuitive design perceptions, accurate design judgments, and convenient design decisions, thus effectively improving their on-site design experiences.
The Distinctive Role of Chemical Composition in Archaeometry. The Case of Apulian Red Figure Pottery
Authors: Giannossa Lorena Carla , Forleo Tiziana , Mangone Annarosa
Published: March 2021

Correlation of the scientific approach to the archaeological investigation and vice versa is considered, for at least the past 30 years, as the best strategy to answer questions in cultural heritage. Many archaeological queries have merged archaeological and scientific studies and have been carried out with a multidisciplinary approach that uses complementary analytical techniques. Here, we focused our efforts on outlining the strong relevance of elemental composition in chemistry and mineralogical investigations to answer important archaeological questions in the case of Apulian red figure pottery. This ceramic class is the most important quantitative handcraft production group of figured pottery in Magna Grecia and the most widespread and commercialized production from the third quarter of the fifth century to the end of the next century. The results obtained indicate that, by exploring chemical elements in the ceramic mixture, it is possible to extract information about provenance, manufacturing processes, originality and restoration techniques.
Development of a Cryogen-Free Compact 3 T Superconducting Magnet for an Electromagnetic Property Measurement System
Authors: Jang Jae Young , Kim Myung Su , Hwang Young Jin , Song Seunghyun , Choi Yojong , Choi Yeon Suk
Published: March 2021

A cryogen-free portable 3 T high-temperature superconducting magnet for an electromagnetic property measurement system has been developed to serve as a user facility at the Korea Basic Science Institute. The metallic insulation method was adopted to reduce the charging delay without sacrificing the self-protecting feature. A genetic-algorithm-aided optimized design was carried out to minimize the superconducting tape consumption while satisfying several design constraints. After the design, the compact high-temperature superconducting magnet composed of eight double-pancake coil modules was wound with high-temperature superconducting tape and stainless steel tape, and integrated with a two-stage cryo-cooler. The 3 T magnet was successfully cooled to approximately 20 K with a cryo-cooler and reached the target field of 3 T without any problems. Long-term measurements and a range of other tests were also implemented to verity the performance of the magnet. Test results demonstrated the feasibility of a cryogen-free portable high-temperature superconducting magnet system for electromagnetic property measurement experiments.
Transformed Structural Properties Method to Determine the Controllability and Observability of Robots
Authors: Martinez Dany Ivan , Rubio José de Jesús , Garcia Victor , Vargas Tomas Miguel , Islas Marco Antonio , Pacheco Jaime , Gutierrez Guadalupe Juliana , Meda-Campaña Jesus Alberto , Mujica-Vargas Dante , Aguilar-Ibañez Carlos
Published: March 2021

Many investigations use a linearization method, and others use a structural properties method to determine the controllability and observability of robots. In this study, we propose a transformed structural properties method to determine the controllability and observability of robots, which is the combination of the linearization and the structural properties methods. The proposed method uses a transformation in the robot model to obtain a linear robot model with the gravity terms and uses the linearization of the gravity terms to obtain the linear robot model; this linear robot model is used to determine controllability and observability. The described combination evades the structural conditions requirement and decreases the approximation error. The proposed method is better than previous methods because the proposed method can obtain more precise controllability and observability results. The modified structural properties method is compared with the linearization method to determine the controllability and observability of three robots.
Study on Influence of Multi-Parameter Variation of Bladed Disk System on Vibration Characteristics
Authors: Pan Honggang , Wu Yunshi , Zhao Tianyu
Published: March 2021

As the main components of the rotor system of aero-engines and other rotating machinery equipment, the bladed disk system has high requirements on its structure design, safety and stability. Taking the rotor disk system of aero-engines as the research object, modal calculation of the rotor disk system was based on the group theory algorithm, and using the fine sand movement on the experimental disk to express the disk vibration shape. The experimental vibration mode is used to compare with the finite element calculation results to verify the reliability of the finite element analysis. Study on the effect of dissonance parameter changes on the bladed disk system vibration characteristics concluded that the vibration mode trends of the blisk system and the disc are, basically, consistent. As the mass of the blade increases, the modal frequencies of the entire blisk system gradually decrease, and the amplitude slightly increases. When the mass increases at different parts of the blade, the effect on the modal frequencies of the bladed disk system are not obvious. When the bladed disk system vibrates at low frequency, the disc will not vibrate and each blade will vibrate irregularly. The bladed disk should be avoided to work in this working area for a long time, so as not to cause fatigue damage or even fracture of some blades.
NFX1, Its Isoforms and Roles in Biology, Disease and Cancer
Authors: Chintala Sreenivasulu , Katzenellenbogen Rachel A.
Published: March 2021

In 1989, two NFX1 protein products were identified as nuclear proteins with the ability to bind to X-box cis-elements. Since that publication, the NFX1 gene and its homologs have been identified, from yeast to humans. This review article summarizes what is known about the NFX1 gene across species. We describe the gene and protein motifs of NFX1 homologs and their functions in cellular biology, then turn to NFX1 in human biology and disease development. In that, we focus on more recent literature about NFX1 and its two splice variants protein products (NFX1-91 and NFX1-123) that are expressed in epithelial cells. We describe new evidence of conserved protein motifs, direct and indirect gene expression regulation, and critical protein-protein interactions. Finally, we stress the emerging roles of these NFX1 splice variants in high-risk human papillomavirus-associated cancers, and the increased expression of the longer splice variant, NFX1-123, found in these cancers.
The 40-Year Mystery of Insect Odorant-Binding Proteins
Authors: Rihani Karen , Ferveur Jean-François , Briand Loïc
Published: March 2021

The survival of insects depends on their ability to detect molecules present in their environment. Odorant-binding proteins (OBPs) form a family of proteins involved in chemoreception. While OBPs were initially found in olfactory appendages, recently these proteins were discovered in other chemosensory and non-chemosensory organs. OBPs can bind, solubilize and transport hydrophobic stimuli to chemoreceptors across the aqueous sensilla lymph. In addition to this broadly accepted “transporter role”, OBPs can also buffer sudden changes in odorant levels and are involved in hygro-reception. The physiological roles of OBPs expressed in other body tissues, such as mouthparts, pheromone glands, reproductive organs, digestive tract and venom glands, remain to be investigated. This review provides an updated panorama on the varied structural aspects, binding properties, tissue expression and functional roles of insect OBPs.
Identification and Biochemical Characterization of High Mobility Group Protein 20A as a Novel Ca2+/S100A6 Target
Authors: Yamamoto Maho , Kondo Rina , Hozumi Haruka , Doi Seita , Denda Miwako , Magari Masaki , Kanayama Naoki , Hatano Naoya , Morishita Ryo , Tokumitsu Hiroshi
Published: March 2021

During screening of protein-protein interactions, using human protein arrays carrying 19,676 recombinant glutathione s-transferase (GST)-fused human proteins, we identified the high-mobility protein group 20A (HMG20A) as a novel S100A6 binding partner. We confirmed the Ca2+-dependent interaction of HMG20A with S100A6 by the protein array method, biotinylated S100A6 overlay, and GST-pulldown assay in vitro and in transfected COS-7 cells. Co-immunoprecipitation of S100A6 with HMG20A from HeLa cells in a Ca2+-dependent manner revealed the physiological relevance of the S100A6/HMG20A interaction. In addition, HMG20A has the ability to interact with S100A1, S100A2, and S100B in a Ca2+-dependent manner, but not with S100A4, A11, A12, and calmodulin. S100A6 binding experiments using various HMG20A mutants revealed that Ca2+/S100A6 interacts with the C-terminal region (residues 311–342) of HMG20A with stoichiometric binding (HMG20A:S100A6 dimer = 1:1). This was confirmed by the fact that a GST-HMG20A mutant lacking the S100A6 binding region (residues 311–347, HMG20A-ΔC) failed to interact with endogenous S100A6 in transfected COS-7 cells, unlike wild-type HMG20A. Taken together, these results identify, for the first time, HMG20A as a target of Ca2+/S100 proteins, and may suggest a novel linkage between Ca2+/S100 protein signaling and HMG20A function, including in the regulation of neural differentiation.
Mechanisms of Primary Membranous Nephropathy
Authors: Gu Yan , Xu Hui , Tang Damu
Published: March 2021

Membranous nephropathy (MN) is an autoimmune disease of the kidney glomerulus and one of the leading causes of nephrotic syndrome. The disease exhibits heterogenous outcomes with approximately 30% of cases progressing to end-stage renal disease. The clinical management of MN has steadily advanced owing to the identification of autoantibodies to the phospholipase A2 receptor (PLA2R) in 2009 and thrombospondin domain-containing 7A (THSD7A) in 2014 on the podocyte surface. Approximately 50–80% and 3–5% of primary MN (PMN) cases are associated with either anti-PLA2R or anti-THSD7A antibodies, respectively. The presence of these autoantibodies is used for MN diagnosis; antibody levels correlate with disease severity and possess significant biomarker values in monitoring disease progression and treatment response. Importantly, both autoantibodies are causative to MN. Additionally, evidence is emerging that NELL-1 is associated with 5–10% of PMN cases that are PLA2R- and THSD7A-negative, which moves us one step closer to mapping out the full spectrum of PMN antigens. Recent developments suggest exostosin 1 (EXT1), EXT2, NELL-1, and contactin 1 (CNTN1) are associated with MN. Genetic factors and other mechanisms are in place to regulate these factors and may contribute to MN pathogenesis. This review will discuss recent developments over the past 5 years.
Eat, Train, Sleep—Retreat? Hormonal Interactions of Intermittent Fasting, Exercise and Circadian Rhythm
Authors: Haupt Sandra , Eckstein Max L. , Wolf Alina , Zimmer Rebecca T. , Wachsmuth Nadine B. , Moser Othmar
Published: March 2021

The circadian rhythmicity of endogenous metabolic and hormonal processes is controlled by a complex system of central and peripheral pacemakers, influenced by exogenous factors like light/dark-cycles, nutrition and exercise timing. There is evidence that alterations in this system may be involved in the pathogenesis of metabolic diseases. It has been shown that disruptions to normal diurnal rhythms lead to drastic changes in circadian processes, as often seen in modern society due to excessive exposure to unnatural light sources. Out of that, research has focused on time-restricted feeding and exercise, as both seem to be able to reset disruptions in circadian pacemakers. Based on these results and personal physical goals, optimal time periods for food intake and exercise have been identified. This review shows that appropriate nutrition and exercise timing are powerful tools to support, rather than not disturb, the circadian rhythm and potentially contribute to the prevention of metabolic diseases. Nevertheless, both lifestyle interventions are unable to address the real issue: the misalignment of our biological with our social time.
miR-30b-5p Downregulation as a Predictive Biomarker of Coronary In-Stent Restenosis
Authors: Gutierrez-Carretero Encarnación , Mayoral-González Isabel , Jesús Morón Francisco , Fernández-Quero Mónica , Domínguez-Rodríguez Alejandro , Ordóñez Antonio , Smani Tarik
Published: March 2021

In-stent restenosis (ISR) is one of the main limitations of percutaneous coronary intervention (PCI) therapy with drug-eluting stents (DES) implantation. The aim of this study was to determine if circulating microRNAs (miRNAs) have diagnostic capability for determining ISR in a cohort of matched patients. Blood samples were collected from 55 patients who underwent previously PCI and were readmitted for a new coronary angiography. Patients were divided into subgroups comprising patients who presented ISR or not (non-ISR). A microarray analysis determined that up to 49 miRNAs were differentially expressed between ISR and non-ISR patients. Of these, 10 miRNAs are related to vascular smooth muscle and endothelial cells proliferation, migration, and differentiation, well-known hallmarks of vascular remodeling. Additionally, we identified that the expression of miR-30b-5p is significantly lower in serum samples of ISR patients, as compared to non-ISR. A further analysis demonstrated that miR-30b-5p provides better values of the receiver operator characteristic curve than other miRNAs and biochemical parameters. Finally, the in-silico analysis suggests that miR-30b-5p is predicted to target 62 genes involved in different signaling pathways involved in vascular remodeling. In conclusion, we determined for the first time that circulating mi-R30b-5p can reliably prognose restenosis in patient with implanted DES, which could be potentially helpful in the establishment of an early diagnosis and therapy of ISR.
Medical Devices for Tremor Suppression: Current Status and Future Directions
Authors: Mo Jiancheng , Priefer Ronny
Published: March 2021

Tremors are the most prevalent movement disorder that interferes with the patient’s daily living, and physical activities, ultimately leading to a reduced quality of life. Due to the pathophysiology of tremor, developing effective pharmacotherapies, which are only suboptimal in the management of tremor, has many challenges. Thus, a range of therapies are necessary in managing this progressive, aging-associated disorder. Surgical interventions such as deep brain stimulation are able to provide durable tremor control. However, due to high costs, patient and practitioner preference, and perceived high risks, their utilization is minimized. Medical devices are placed in a unique position to bridge this gap between lifestyle interventions, pharmacotherapies, and surgical treatments to provide safe and effective tremor suppression. Herein, we review the mechanisms of action, safety and efficacy profiles, and clinical applications of different medical devices that are currently available or have been previously investigated for tremor suppression. These devices are primarily noninvasive, which can be a beneficial addition to the patient’s existing pharmacotherapy and/or lifestyle intervention.
Dissecting the Molecular Determinants of GABAA Receptors Current Rundown, a Hallmark of Refractory Human Epilepsy
Authors: Cifelli Pierangelo , Di Angelantonio Silvia , Alfano Veronica , Morano Alessandra , De Felice Eleonora , Aronica Eleonora , Ruffolo Gabriele , Palma Eleonora
Published: March 2021

GABAA receptors-(Rs) are fundamental for the maintenance of an efficient inhibitory function in the central nervous system (CNS). Their dysfunction is associated with a wide range of CNS disorders, many of which characterized by seizures and epilepsy. Recently, an increased use-dependent desensitization due to a repetitive GABA stimulation (GABAA current rundown) of GABAARs has been associated with drug-resistant temporal lobe epilepsy (TLE). Here, we aimed to investigate the molecular determinants of GABAA current rundown with two different heterologous expression systems (Xenopus oocytes and human embryonic kidney cells; HEK) which allowed us to manipulate receptor stoichiometry and to study the GABAA current rundown on different GABAAR configurations. To this purpose, we performed electrophysiology experiments using two-electrode voltage clamp in oocytes and confirming part of our results in HEK. We found that different degrees of GABAA current rundown can be associated with the expression of different GABAAR β-subunits reaching the maximum current decrease when functional α1β2 receptors are expressed. Furthermore, the blockade of phosphatases can prevent the current rundown observed in α1β2 GABAARs. Since GABAAR represents one important therapeutic target in the treatment of human epilepsy, our results could open new perspectives on the therapeutic management of drug-resistant patients showing a GABAergic impairment.
Cognitive Issues in Pediatric Multiple Sclerosis
Authors: Portaccio Emilio , De Meo Ermelinda , Bellinvia Angelo , Amato Maria Pia
Published: March 2021

Multiple sclerosis (MS) is one of the leading causes of disability in young adults. The onset of MS during developmental age makes pediatric patients particularly susceptible to cognitive impairment, resulting from both disease-related damage and failure of age-expected brain growth. Despite different test batteries and definitions, cognitive impairment has been consistently reported in approximately one-third of pediatric patients with MS. However, the lack of a uniform definition of cognitive impairment and the adoption of different test batteries have led to divergent results in terms of cognitive domains more frequently affected across the cohorts explored. This heterogeneity has hampered large international collaborative studies. Moreover, research aimed at the identification of risk factors (e.g., demographic, clinical, and radiological features) or protective factors (e.g., cognitive reserve, leisure activities) for cognitive decline is still scanty. Mood disorders, such as depression and anxiety, can be detected in these patients alongside cognitive decline or in isolation, and can negatively affect quality of life scores as well as academic performances. By using MRI, cognitive impairment was attributed to damage to specific brain compartments as well as to abnormal network activation patterns. However, multimodal MRI studies are still needed in order to assess the contribution of each MRI metric to cognitive impairment. Importantly, longitudinal studies have recently demonstrated failure of age-expected brain growth and of white matter (WM) and gray matter (GM) maturation plays a relevant role in determining cognitive dysfunction, in addition to MS-related direct damage. Whether these growth retardations might result in specific cognitive profiles according to the age at disease onset has not been studied, yet. A better characterization of cognitive profiles in pediatric MS patients, as well as the definition of neuroanatomical substrates of cognitive impairment and their longitudinal evolution are needed to develop efficient therapeutic strategies against cognitive impairment in this patient population.