bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus
Authors: Yue Hao , Huang Li-Ping , Lu Ding-Yi-Hui , Zhang Zhan-Hong , Zhang Zhuo , Zhang De-Yong , Zheng Li-Min , Gao Yang , Tan Xin-Qiu , Zhou Xu-Guo , Shi Xiao-Bin , Liu Yong
Published: June 2021

Tomato chlorosis virus(ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whiteflyBemisia tabaciMediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus andB. tabaciis very limited. To understand the molecular mechanism of the interaction between tomato, ToCV andB. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection ofB. tabaciand ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection ofB. tabaciand ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV andB. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.
Modulation of Neural Microcircuits That Control Complex Dynamics in Olfactory Networks
Authors: Huang Zhenbo , Tatti Roberta , Loeven Ashley M. , Landi Conde Daniel R. , Fadool Debra Ann
Published: June 2021

Neuromodulation influences neuronal processing, conferring neuronal circuits the flexibility to integrate sensory inputs with behavioral states and the ability to adapt to a continuously changing environment. In this original research report, we broadly discuss the basis of neuromodulation that is known to regulate intrinsic firing activity, synaptic communication, and voltage-dependent channels in the olfactory bulb. Because the olfactory system is positioned to integrate sensory inputs with information regarding the internal chemical and behavioral state of an animal, how olfactory information is modulated provides flexibility in coding and behavioral output. Herein we discuss how neuronal microcircuits control complex dynamics of the olfactory networks by homing in on a special class of local interneurons as an example. While receptors for neuromodulation and metabolic peptides are widely expressed in the olfactory circuitry, centrifugal serotonergic and cholinergic inputs modulate glomerular activity and are involved in odor investigation and odor-dependent learning. Little is known about how metabolic peptides and neuromodulators control specific neuronal subpopulations. There is a microcircuit between mitral cells and interneurons that is comprised of deep-short-axon cells in the granule cell layer. These local interneurons express pre-pro-glucagon (PPG) and regulate mitral cell activity, but it is unknown what initiates this type of regulation. Our study investigates the means by which PPG neurons could be recruited by classical neuromodulators and hormonal peptides. We found that two gut hormones, leptin and cholecystokinin, differentially modulate PPG neurons. Cholecystokinin reduces or increases spike frequency, suggesting a heterogeneous signaling pathway in different PPG neurons, while leptin does not affect PPG neuronal firing. Acetylcholine modulates PPG neurons by increasing the spike frequency and eliciting bursts of action potentials, while serotonin does not affect PPG neuron excitability. The mechanisms behind this diverse modulation are not known, however, these results clearly indicate a complex interplay of metabolic signaling molecules and neuromodulators that may fine-tune neuronal microcircuits.
Phenotypic and Genotypic Characterization of DEPDC5-Related Familial Focal Epilepsy: Case Series and Literature Review
Authors: Zhang Xuan , Huang Zhaoyang , Liu Jianghong , Li Mingyu , Zhao Xiaoling , Ye Jing , Wang Yuping
Published: June 2021

Mutations in the disheveled, Egl-10 and domain-containing protein 5 (DEPDC5) recently have been identified as a common cause of focal epilepsy syndromes. The association between phenotype and genotype of DEPDC5 mutation has not been adequately characterized. We studied four families with familial focal epilepsy carrying DEPDC5 mutations. Four novel DEPDC5 mutations were identified by next-generation sequencing, including two missense mutations (c.1729 >A and c.3260G>A), one splicing mutation (c.280-1G>A), and one frameshift mutation (c.515_516delinsT). We found that patients carrying different DEPDC5 mutation have different clinical manifestations. Incomplete penetrance is a prominent feature of DEPDC5-related epilepsy, with the rate of penetrance ranging from 25 to 100%. About 21.4% of patients with DEPDC5-related familial focal epilepsy are refractory to treatments. We further reviewed the correlation of genotype and phenotype in all previous literature regarding DEPDC5-related epilepsy. Our study suggested that the type of DEPDC5 mutation might provide important information for the prognosis evaluation.
The Usefulness of Functional Near-Infrared Spectroscopy for the Assessment of Post-Stroke Depression
Authors: Koyanagi Masahiko , Yamada Mai , Higashi Toshio , Mitsunaga Wataru , Moriuchi Takefumi , Tsujihata Mitsuhiro
Published: June 2021

Background: Post-stroke depression (PSD) is the most common mood disorder following stroke and is also the main factor that limits the recovery and rehabilitation of patients with stroke. The prevalence of PSD is ~30%. Since there is no gold standard for the diagnosis and evaluation of PSD, it is important to raise awareness of PSD and to establish methods for its evaluation, early diagnosis, and treatment. In the field of psychiatry, functional near-infrared spectroscopy (fNIRS) has been used as a diagnostic tool for the measurement of oxygenated hemoglobin (oxy-Hb). This study aimed to assess whether fNIRS could be applied in the diagnosis and evaluation of PSD.

Methods: We recruited 45 patients with stroke, who were admitted to Nagasaki Kita Hospital between May 2015 and April 2019. The 17-item Hamilton Rating Scale for Depression (HAMD17), which is considered to be a useful screening and evaluation tool for PSD, was used for the assessment of patients after stroke; moreover, oxy-Hb was measured in the pre-frontal cortex. The subjects were divided into two groups: the depressed group (n = 13) and the non-depressed group (n = 32). We evaluated the correlation between the oxy-Hb integral values and HAMD17 scores.

Results: We investigated the relationship between the oxy-Hb integral values and HAMD17 total scores, and found a negative correlation between them (ρ = −0.331, P < 0.005). There was a significant difference in the oxy-Hb integral values during the activation task period between the depressed and non-depressed groups (3.16 ± 2.7 and 1.71 ± 2.4, respectively; P = 0.040). The results indicated that the patients of the depressed group showed lower oxy-Hb integral values and lower activation in the frontal lobe in comparison with the patients of the non-depressed group.

Conclusion: The present study highlights that the measurement of oxy-Hb by using fNIRS is a useful methodology for the diagnosis of PSD in patients after stroke.
FlywheelTools: Data Curation and Manipulation on the Flywheel Platform
Authors: Tapera Tinashe M. , Cieslak Matthew , Bertolero Max , Adebimpe Azeez , Aguirre Geoffrey K. , Butler Ellyn R. , Cook Philip A. , Davila Diego , Elliott Mark A. , Linguiti Sophia , Murtha Kristin , Tackett William , Detre John A. , Satterthwaite Theodore D.
Published: June 2021

The recent and growing focus on reproducibility in neuroimaging studies has led many major academic centers to use cloud-based imaging databases for storing, analyzing, and sharing complex imaging data. Flywheel is one such database platform that offers easily accessible, large-scale data management, along with a framework for reproducible analyses through containerized pipelines. The Brain Imaging Data Structure (BIDS) is the de facto standard for neuroimaging data, but curating neuroimaging data into BIDS can be a challenging and time-consuming task. In particular, standard solutions for BIDS curation are limited on Flywheel. To address these challenges, we developed “FlywheelTools,” a software toolbox for reproducible data curation and manipulation on Flywheel. FlywheelTools includes two elements: fw-heudiconv, for heuristic-driven curation of data into BIDS, and flaudit, which audits and inventories projects on Flywheel. Together, these tools accelerate reproducible neuroscience research on the widely used Flywheel platform.
Quantitative Label-Free Proteomic Analysis of Milk Fat Globule Membrane in Donkey and Human Milk
Authors: Zhang Xinhao , Jiang Bo , Ji Chuanliang , Li Haijing , Yang Li , Jiang Guimiao , Wang Yantao , Liu Guangyuan , Liu Guiqin , Min Lingjiang , Zhao Fuwei
Published: June 2021

Previous studies have found donkey milk (DM) has the similar compositions with human milk (HM) and could be used as a potential hypoallergenic replacement diet for babies suffering from cow's milk allergy. Milk fat globule membrane (MFGM) proteins are involved in many biological functions, behaving as important indicators of the nutritional quality of milk. In this study, we used label-free proteomics to quantify the differentially expressed MFGM proteins (DEP) between DM (in 4–5 months of lactation) and HM (in 6–8 months of lactation). In total, 293 DEP were found in these two groups. Gene Ontology (GO) enrichment analysis revealed that the majority of DEP participated in regulation of immune system process, membrane invagination and lymphocyte activation. Several significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were determined for the DEP, such as lysosome, galactose metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. Our study may provide valuable information in the composition of MFGM proteins in DM and HM, and expand our knowledge of different biological functions between DM and HM.
Dental Malocclusion and Its Relation to the Podal System
Authors: Cabrera-Domínguez María E. , Domínguez-Reyes Antonia , Pabón-Carrasco Manuel , Pérez-Belloso Ana J. , Coheña-Jiménez Manuel , Galán-González Antonio F.
Published: June 2021

Background and Objective: This study analyzes the possibility that Stomatognathic and Postural systems are related by muscle chains. Malocclusion may influence the posture, contact between the foot and the ground, center of mass, footprint or vice-versa. This study aimed to verify whether there is a relationship between dental occlusion and podal system.

Materials and Methods: A cross-cutting, descriptive study was carried out on 409 children (222 boys and 187 girls) between 8 and 14 years old. Dental occlusion was assessed on the sagittal plane (Angle's classification) the contact between the foot and the ground and the center of mass were evaluated using a stabilometric platform.

Results: There was a statistically significant relationship between the plantigrade phase, the contact surface area and center of gravity. There was a prevalence of molar and canine Angle's class II malocclusion. In molar class II, an anterior center of gravity was predominant, in class I it was centered and in class III, it was posterior. There was significant correlation between malocclusions and the FPI (foot posture index) of the left foot and the height of the scaphoid in the right foot (P < 0.001).

Conclusions: Some authors agree with our results. There is still much uncertainty in terms of showing a relationship between both systems. In addition, there is scarce scientific evidence on the topic. Some kind of relationship between the two systems has been proven. Studies that evaluate a group of subjects in a longitudinal manner are necessary to enable the changes taking place in both systems to be defined.
Real-Time PCR Assay for the Diagnosis and Quantification of Co-infections by Diaporthe batatas and Diaporthe destruens in Sweet Potato
Authors: Fujiwara Kazuki , Kobayashi Yuki O. , Usui Manami , Nishioka Kazuya , Nakamura Misa , Kawano Shinji , Okada Yoshihiro , Kobayashi Akira , Miyasaka Atsushi , Hirayae Kazuyuki , Kushima Yoshiyuki , Nishi Yatsuka , Inoue Hiroyoshi
Published: June 2021

Foot rot disease caused by Diaporthe destruens (formerly Plenodomus destruens) has become a major concern for the production of sweet potato [Ipomoea batatas (L.) Lam.] in Japan. A related fungus Diaporthe batatas, which causes dry rot disease of sweet potato, is native and is widespread in fields in Japan. The similar characteristics of these two pathogens pose a challenge for conventional disease diagnosis. Currently, there are no effective molecular measures for identifying and distinguishing D. destruens and D. batatas. Here, we demonstrate a real-time PCR assay that distinguishes and quantifies D. batatas and D. destruens from co-infected sweet potato. The assay was performed with various simulated DNA combinations of D. batatas and D. destruens ranging from 1:1 to 1:100000. The assay was also used with the ratios of D. batatas: D. destruens: sweet potato DNA ranging from 1:1:1 to 1:1:100000. These assays produced a specific amplification product for each of the pathogens, and quantified the fungal biomass over the entire range tested without detecting false positives. The assay was validated by using infected sweet potato collected from various fields; it showed sufficient sensitivity and specificity to quantify and distinguish D. batatas and D. destruens from these field samples. Thus, our real-time PCR assay would be a useful tool for diagnosis of D. batatas and D. destruens and is expected to provide the foundation for the design of integrated disease management strategies for foot rot disease in sweet potato.
Cross-Cultural Differences in Adopting Social Cognitive Career Theory at Student Employability in PLS-SEM: The Mediating Roles of Self-Efficacy and Deep Approach to Learning
Authors: Zhao Wen-Xuan , Peng Michael Yao-Ping , Liu Fang
Published: June 2021

Unable to keep pace with the expectations of employers and societies that are constantly changing around the world, higher education policy and talent training have given rise to a gap between university education and employment. However, the higher education and industrial needs derived from technological progress have changed the development in country. This study aims to verify the learning method of Taiwanese vs. Malaysian university students and examines the relationship between teacher knowledge transfer and student employability from the perspective of a social cognitive career theory. In this study, 619 copies of questionnaires from the Taiwanese sample group and 443 copies of questionnaires from the Malaysian sample group were collected in total to compare the two sample groups in the development of student employability. The results indicate that teacher knowledge transfer has significant positive correlations with self-efficacy and a deep approach to learning and student employability, and the self-efficacy and a deep approach to learning have significant positive correlations with student employability in the Taiwanese sample. In Malaysia, except for the path between teacher knowledge transfer and student employability, all paths were significant and positively related. Finally, according to our results and findings, this study proposes several insights with practical and theoretical implications for future study.
Case of Asperger's Syndrome and Lesion of the Right Amygdala: Deficits in Implicit and Explicit Fearful Face Recognition
Authors: Koelkebeck Katja , Bauer Jochen , Suslow Thomas , Ohrmann Patricia
Published: June 2021

Introduction: Studies of brain-damaged patients revealed that amygdala lesions cause deficits in the processing and recognition of emotional faces. Patients with autism spectrum disorders (ASD) have similar deficits also related to dysfunctions of the limbic system including the amygdala.

Methods: We investigated a male patient who had been diagnosed with Asperger's syndrome. He also presented with a lesion of the right mesial temporal cortex, including the amygdala. We used functional magnetic resonance imaging (fMRI) to investigate neuronal processing during a passive viewing task of implicit and explicit emotional faces. Clinical assessment included a facial emotion recognition task.

Results: There was no amygdala activation on both sides during the presentation of masked emotional faces compared to the no-face control condition. Presentation of unmasked happy and angry faces activated the left amygdala compared to the no-face control condition. There was no amygdala activation in response to unmasked fearful faces on both sides. In the facial emotion recognition task, the patient biased positive and neutral expressions as negative.

Conclusions: This case report describes a male patient with right amygdala damage and an ASD. He displayed a non-response of the amygdala to fearful faces and tended to misinterpret fearful expressions. Moreover, a non-reactivity of both amygdalae to emotional facial expressions at an implicit processing level was revealed. It is discussed whether the deficient implicit processing of facial emotional information and abnormalities in fear processing could contribute and aggravate the patient's impairments in social behavior and interaction.
Investigating the Dimensionality of Early Numeracy Using the Bifactor Exploratory Structural Equation Modeling Framework
Authors: Dierendonck Christophe , de Chambrier Anne-Françoise , Fagnant Annick , Luxembourger Christophe , Tinnes-Vigne Mélanie , Poncelet Débora
Published: June 2021

The few studies that have analyzed the factorial structure of early number skills have mainly used confirmatory factor analysis (CFA) and have yielded inconsistent results, since early numeracy is considered to be unidimensional, multidimensional or even underpinned by a general factor. Recently, the bifactor exploratory structural equation modeling (bifactor-ESEM)—which has been proposed as a way to overcome the shortcomings of both the CFA and the exploratory structural equation modeling (ESEM)—proved to be valuable to account for the multidimensionality and the hierarchical nature of several psychological constructs. The present study is the first to investigate the dimensionality of early number skills measurement through the application of the bifactor-ESEM framework. Using data from 644 prekindergarten and kindergarten children (4 to 6 years old), several competing models were contrasted: the one-factor CFA model; the independent cluster model (ICM-CFA); the exploratory structural equation modeling (ESEM); and their bifactor counterpart (bifactor-CFA and bifactor-ESEM, respectively). Results indicated acceptable fit indexes for the one-factor CFA and the ICM-CFA models and excellent fit for the others. Among these, the bifactor-ESEM with one general factor and three specific factors (Counting, Relations, Arithmetic) not only showed the best model fit, but also the best coherent factor loadings structure and full measurement invariance across gender. The bifactor-ESEM appears relevant to help disentangle and account for general and specific factors of early numerical ability. While early numerical ability appears to be mainly underpinned by a general factor whose exact nature still has to be determined, this study highlights that specific latent dimensions with substantive value also exist. Identifying these specific facets is important in order to increase quality of early numerical ability measurement, predictive validity, and for practical implications.
Failure to Find Altruistic Food Sharing in Rats
Authors: Wan Haoran , Kirkman Cyrus , Jensen Greg , Hackenberg Timothy D.
Published: June 2021

Prior research has found that one rat will release a second rat from a restraint in the presence of food, thereby allowing that second rat access to food. Such behavior, clearly beneficial to the second rat and costly to the first, has been interpreted as altruistic. Because clear demonstrations of altruism in rats are rare, such findings deserve a careful look. The present study aimed to replicate this finding, but with more systematic methods to examine whether, and under what conditions, a rat might share food with its cagemate partner. Rats were given repeated choices between high-valued food (sucrose pellets) and 30-s social access to a familiar rat, with the (a) food size (number of food pellets per response), and (b) food motivation (extra-session access to food) varied across conditions. Rats responded consistently for both food and social interaction, but at different levels and with different sensitivity to the food-access manipulations. Food production and consumption was high when food motivation was also high (food restriction) but substantially lower when food motivation was low (unlimited food access). Social release occurred at moderate levels, unaffected by the food-based manipulations. When food was abundant and food motivation low, the rats chose food and social options about equally often, but sharing (food left unconsumed prior to social release) occurred at low levels across sessions and conditions. Even under conditions of low food motivation, sharing occurred on only 1% of the sharing opportunities. The results are therefore inconsistent with claims in the literature that rats are altruistically motivated to share food with other rats.
Addressing the Mental and Emotional Health Impacts of COVID-19 on Children and Adolescents: Lessons From HIV/AIDS
Authors: Banati Prerna , Idele Priscilla
Published: June 2021

The COVID-19 pandemic has led to lasting mental health and psychosocial consequences just as were experienced with the HIV epidemic. A rapid review of published systematic reviews on HIV/AIDS and mental health outcomes and responses among children and adolescents was used to identify lessons for the COVID-19 pandemic response. The review found that HIV/AIDS responses to promote mental health, prevent ill-health and treat mental health conditions included diverse interventions at the structural or national, community, household and individual levels. Some of these responses can be easily replicated, others require substantial adaptation, and some can inform development of new innovative offline and online responses to mitigate impact of COVID-19 on mental health of children and adolescents. Programs that mitigate economic impacts including child grants, income generating activities for caregivers, food distribution, health care vouchers, and other economic empowerment interventions can be replicated with minor adjustments. Helplines for vulnerable or abused children and shelters for victims of gender-based violence can be scaled up to respond to the COVID pandemic, with minimal adaptation to adhere to prevention of contagion. Mass media campaigns to combat stigma and discrimination were successfully employed in the HIV response, and similar interventions could be developed and applied in the COVID context. Some programs will need more substantial adjustments. In health facilities, mainstreaming child-sensitive mental health training of frontline workers and task sharing/shifting to community volunteers and social workers as was done for HIV with community health workers, could advance mental illness detection, particularly among abuse victims, but requires adaptation of protocols. At the community and household levels, expansion of parenting programs can help caregivers navigate negative mental health effects on children, however, these are not often operating at scale, nor well-linked to services. Programs requiring innovation include converting adolescent and youth safe physical spaces into virtual spaces particularly for at-risk girls and young women; organizing virtual community support groups, conversations, and developing online resources. Re-opening of schools and introduction of health and hygiene policies, provides another opportunity for innovation - to provide mental health and psychosocial support to all children as a standard package of care and practice.
Fatigue and Its Association With Quality of Life Among Carers of Patients Attending Psychiatric Emergency Services During the COVID-19 Pandemic
Authors: Ji Xiao , Li Wen , Zhu Hui , Zhang Ling , Cheung Teris , Ng Chee H. , Xiang Yu-Tao
Published: June 2021

Aims: Carers of psychiatric patients often suffered from mental and physical burden during the coronavirus disease 2019 (COVID-19) pandemic due to the lack of mental health services. This study investigated the pattern of fatigue and its association with quality of life (QOL) among the carers of patients attending psychiatric emergency services during the COVID-19 pandemic.

Methods: In this cross-sectional study, carers of patients attending psychiatric emergency services during the COVID-19 pandemic were consecutively included. Fatigue, insomnia symptoms, depressive symptoms, and QOL were assessed with standardized instruments.

Results: A total of 496 participants were included. The prevalence of fatigue was 44.0% (95% CI = 39.6–48.4%). Multivariate logistic regression analysis revealed that fatigue was positively associated with higher education level (OR = 1.92, P < 0.01) and more severe depressive (OR = 1.18, P < 0.01) and insomnia symptoms (OR = 1.11, P < 0.01). ANCOVA analysis revealed that the QOL was significantly lower in carers with fatigue compared with those without (P = 0.03).

Conclusions: Fatigue was common among carers of patients attending psychiatric emergency services during the COVID-19 pandemic. Considering the adverse impact of fatigue on QOL and other health outcomes, routine screening and appropriate intervention for fatigue are warranted for this subpopulation.
A Differential Modulation Scheme for Metasurface-Based Terahertz Communications
Authors: Guo Shuaishuai , Ye Jia , Zhang Peng
Published: June 2021

Metasurface-based terahertz (THz) modulators, which carry information by changing the spatial pattern of the metasurface at every symbol time, are an important component to establish low-cost low-energy–consuming THz communication systems. This paper proposes a differential spatial THz modulation (DSTM) scheme for metasurface-assisted THz communications. In DSTM, the metasurface pattern activation orders are employed to carry information, instead of the metasurface patterns themselves. In this way, the DSTM receiver can perform differential detection without knowing the metasurface response to incident THz signals and the channel state information. The proposed DSTM scheme is applicable to all kinds of metasurfaces enabling THz communications, including transmissive metasurfaces and reflective metasurfaces. For high-rate DSTM systems, we propose an efficient bits-to-activation-order mapping method and a low-complexity detection method. Simulations are conducted to demonstrate the performance of the proposed scheme. It is shown that the proposed scheme pays acceptable penalty compared to the non-differential modulation scheme with coherent detection, which needs to know the metasurface response to the incident signals as well as all the channel state information.
Assistive Navigation Using Deep Reinforcement Learning Guiding Robot With UWB/Voice Beacons and Semantic Feedbacks for Blind and Visually Impaired People
Authors: Lu Chen-Lung , Liu Zi-Yan , Huang Jui-Te , Huang Ching-I , Wang Bo-Hui , Chen Yi , Wu Nien-Hsin , Wang Hsueh-Cheng , Giarré Laura , Kuo Pei-Yi
Published: June 2021

Facilitating navigation in pedestrian environments is critical for enabling people who are blind and visually impaired (BVI) to achieve independent mobility. A deep reinforcement learning (DRL)–based assistive guiding robot with ultrawide-bandwidth (UWB) beacons that can navigate through routes with designated waypoints was designed in this study. Typically, a simultaneous localization and mapping (SLAM) framework is used to estimate the robot pose and navigational goal; however, SLAM frameworks are vulnerable in certain dynamic environments. The proposed navigation method is a learning approach based on state-of-the-art DRL and can effectively avoid obstacles. When used with UWB beacons, the proposed strategy is suitable for environments with dynamic pedestrians. We also designed a handle device with an audio interface that enables BVI users to interact with the guiding robot through intuitive feedback. The UWB beacons were installed with an audio interface to obtain environmental information. The on-handle and on-beacon verbal feedback provides points of interests and turn-by-turn information to BVI users. BVI users were recruited in this study to conduct navigation tasks in different scenarios. A route was designed in a simulated ward to represent daily activities. In real-world situations, SLAM-based state estimation might be affected by dynamic obstacles, and the visual-based trail may suffer from occlusions from pedestrians or other obstacles. The proposed system successfully navigated through environments with dynamic pedestrians, in which systems based on existing SLAM algorithms have failed.
Glycyrrhizin Attenuates Salmonella Typhimurium-Induced Tissue Injury, Inflammatory Response, and Intestinal Dysbiosis in C57BL/6 Mice
Authors: Wang Baikui , Ye Xiaolin , Zhou Yuanhao , Zhao Pengwei , Mao Yulong
Published: June 2021

Salmonellae are one of the most important foodborne pathogens, which threaten the health of humans and animals severely. Glycyrrhizin (GL) has been proven to exhibit anti-inflammatory and tissue-protective properties. Here, we investigated the effects of GL on tissue injury, inflammatory response, and intestinal dysbiosis in Salmonella Typhimurium-infected mice. Results showed that GL or gentamicin (GM) significantly (P < 0.05) alleviated ST-induced splenomegaly indicated by the decreased spleen index, injury of liver and jejunum indicated by the decreased hepatocytic apoptosis, and the increased jejunal villous height. GL significantly (P < 0.05) increased secretion of inflammatory cytokines (IFN-γ, IL-12p70, IL-6, and IL-10) in spleen and IL-12p40 mRNA expression in liver. Meanwhile, GL or GM pre-infection treatments significantly (P < 0.05) decreased ST-induced pro-inflammatory cytokine (IFN-γ, TNF-α, and IL-6) expression in both spleen and liver and increased (P < 0.05) anti-inflammatory cytokine IL-10 secretion in spleen. Furthermore, GL or GM pre-infection treatment also regulates the diversities and compositions of intestinal microbiota and decreased the negative connection among the intestinal microbes in ST-infected mice. The above findings indicate that GL alleviates ST-induced splenomegaly, hepatocytic apoptosis, injury of jejunum and liver, inflammatory response of liver and spleen, and intestinal dysbacteriosis in mice.
Molecular Evidence of Bartonella melophagi in Ticks in Border Areas of Xinjiang, China
Authors: Ni Jun , Ren Qiaoyun , Lin Hanliang , Aizezi Malike , Luo Jin , Luo Yi , Ma Zhan , Chen Ze , Liu Wenge , Guo Junhui , Qu Zhiqiang , Xu Xiaofeng , Wu Zegong , Tan Yangchun , Wang Jinming , Li Youquan , Guan Guiquan , Luo Jianxun , Yin Hong , Liu Guangyuan
Published: June 2021

Bartonella are gram-negative intracellular bacteria; certain species of Bartonella can cause diseases in mammals and humans. Ticks play a major role in the transmission of Bartonella. Xinjiang is the largest province in China according to land area and has one-third of the tick species in China; the infection rate of Bartonella in ticks in the Xinjiang border areas has not been studied in detail. Therefore, this study investigated tick infections by Bartonella in Xinjiang border areas, and the purpose of the study was to fill in gaps in information regarding the genetic diversity of tick infections by Bartonella in Xinjiang. We tested 1,549 tick samples from domestic animals (sheep and cattle) for Bartonella using ribC-PCR. Positive samples from the ribC-PCR assay for Bartonella spp. were further subjected to PCR assays targeting the ITS, rpoB and gltA genes followed by phylogenetic analyses. Bartonella DNA was detected in 2.19% (34/1,549) of tick samples, and the ITS, rpoB and gltA genes of ribC gene-positive samples were amplified to identify nine samples of Bartonella melophagi. In this study, molecular analysis was used to assess the presence and genetic diversity of B. melophagi in ticks collected from sheep and cattle from Xinjiang, China. This study provides new information on the presence and identity of B. melophagi in ticks from sheep and cattle.
Nutrigenomics of Dietary Lipids
Authors: Bordoni Laura , Petracci Irene , Zhao Fanrui , Min Weihong , Pierella Elisa , Assmann Taís Silveira , Martinez J Alfredo , Gabbianelli Rosita
Published: June 2021

Dietary lipids have a major role in nutrition, not only for their fuel value, but also as essential and bioactive nutrients. This narrative review aims to describe the current evidence on nutrigenomic effects of dietary lipids. Firstly, the different chemical and biological properties of fatty acids contained both in plant- and animal-based food are illustrated. A description of lipid bioavailability, bioaccessibility, and lipotoxicity is provided, together with an overview of the modulatory role of lipids as pro- or anti-inflammatory agents. Current findings concerning the metabolic impact of lipids on gene expression, epigenome, and gut microbiome in animal and human studies are summarized. Finally, the effect of the individual’s genetic make-up on lipid metabolism is described. The main goal is to provide an overview about the interaction between dietary lipids and the genome, by identifying and discussing recent scientific evidence, recognizing strengths and weaknesses, to address future investigations and fill the gaps in the current knowledge on metabolic impact of dietary fats on health.
β-Elemene Suppresses Obesity-Induced Imbalance in the Microbiota-Gut-Brain Axis
Authors: Zhou Yingyu , Qiu Wanyi , Wang Yimei , Wang Rong , Takano Tomohiro , Li Xuyang , Zhu Zhangliang , Nakajima-Adachi Haruyo , Tanokura Masaru , Hachimura Satoshi , Miyakawa Takuya
Published: June 2021

As a kind of metabolically triggered inflammation, obesity influences the interplay between the central nervous system and the enteral environment. The present study showed that β-elemene, which is contained in various plant substances, had effects on recovering the changes in metabolites occurring in high-fat diet (HFD)-induced obese C57BL/6 male mice brains, especially in the prefrontal cortex (PFC) and hippocampus (HIP). β-elemene also partially reversed HFD-induced changes in the composition and contents of mouse gut bacteria. Furthermore, we evaluated the interaction between cerebral metabolites and intestinal microbiota via Pearson correlations. The prediction results suggested that Firmicutes were possibly controlled by neuron integrity, cerebral inflammation, and neurotransmitters, and Bacteroidetes in mouse intestines might be related to cerebral aerobic respiration and the glucose cycle. Such results also implied that Actinobacteria probably affected cerebral energy metabolism. These findings suggested that β-elemene has regulatory effects on the imbalanced microbiota-gut-brain axis caused by obesity and, therefore, would contribute to the future study in on the interplay between cerebral metabolites from different brain regions and the intestinal microbiota of mice.
MicroRNAs Targeting HIF-2α, VEGFR1 and/or VEGFR2 as Potential Predictive Biomarkers for VEGFR Tyrosine Kinase and HIF-2α Inhibitors in Metastatic Clear-Cell Renal Cell Carcinoma
Authors: Kinget Lisa , Roussel Eduard , Verbiest Annelies , Albersen Maarten , Rodríguez-Antona Cristina , Graña-Castro Osvaldo , Inglada-Pérez Lucía , Zucman-Rossi Jessica , Couchy Gabrielle , Job Sylvie , de Reyniès Aurélien , Laenen Annouschka , Baldewijns Marcella , Beuselinck Benoit
Published: June 2021

Metastatic clear-cell renal cell carcinoma (m-ccRCC) is characterized by increased hypoxia-induced factor (HIF)-2α and vascular endothelial growth factor receptor (VEGFR)-dependent angiogenesis through loss of function of the von Hippel–Lindau protein. VEGFR tyrosine kinase inhibitors (VEGFR-TKIs) are a cornerstone of m-ccRCC treatment, and new treatments targeting HIF-2α are currently under investigation. However, predictive biomarkers for these treatments are lacking. In this retrospective cohort study including 109 patients treated with VEGFR-targeted therapies as first-line treatment, we aimed to study the possible predictive function of microRNAs (miRNAs) targeting HIF-2α, VEGFR1 and VEGFR2. We selected miRNAs inversely correlated with HIF-2α, VEGFR1 and/or VEGFR2 expression and with predicted target sites in the respective genes and subsequently studied their impact on therapeutic outcomes. We identified four miRNAs (miR-34c-5p, miR-221-3p, miR-222-3p and miR-3529-3p) inversely correlated with VEGFR1 and/or VEGFR2 expression and associated with tumor shrinkage and progression-free survival (PFS) upon treatment with VEGFR-TKIs, highlighting the potential predictive value of these miRNAs. Moreover, we identified three miRNAs (miR-185-5p, miR-223-3p and miR-3529-3p) inversely correlated with HIF-2α expression and associated with tumor shrinkage and PFS upon treatment with VEGFR-TKIs. These three miRNAs can have a predictive value not only upon treatment with VEGFR-TKIs but possibly also upon treatment with the upcoming HIF-2α inhibitor belzutifan.
Hyperthermic Intraperitoneal Chemotherapy: A Critical Review
Authors: Ceelen Wim , Demuytere Jesse , de Hingh Ignace
Published: June 2021

With increasing awareness amongst physicians and improved radiological imaging techniques, the peritoneal cavity is increasingly recognized as an important metastatic site in various malignancies. Prognosis of these patients is usually poor as traditional treatment including surgical resection or systemic treatment is relatively ineffective. Intraperitoneal delivery of chemotherapeutic agents is thought to be an attractive alternative as this results in high tumor tissue concentrations with limited systemic exposure. The addition of hyperthermia aims to potentiate the anti-tumor effects of chemotherapy, resulting in the concept of heated intraperitoneal chemotherapy (HIPEC) for the treatment of peritoneal metastases as it was developed about 3 decades ago. With increasing experience, HIPEC has become a safe and accepted treatment offered in many centers around the world. However, standardization of the technique has been poor and results from clinical trials have been equivocal. As a result, the true value of HIPEC in the treatment of peritoneal metastases remains a matter of debate. The current review aims to provide a critical overview of the theoretical concept and preclinical and clinical study results, to outline areas of persisting uncertainty, and to propose a framework to better define the role of HIPEC in the treatment of peritoneal malignancies.
Recent Advances in Solid-Phase Extraction (SPE) Based on Molecularly Imprinted Polymers (MIPs) for Analysis of Hormones
Authors: Mpupa Anele , Selahle Shirley Kholofelo , Mizaikoff Boris , Nomngongo Philiswa Nosizo
Published: June 2021

Steroid hormones are active substances that are necessary in the normal functioning of all physiological activities in the body, such as sexual characteristics, metabolism, and mood control. They are also widely used as exogenous chemicals in medical and pharmaceutical applications as treatments and at times growth promoters in animal farming. The vast application of steroid hormones has resulted in them being found in different matrices, such as food, environmental, and biological samples. The presence of hormones in such matrices means that they can easily come into contact with humans and animals as exogenous compounds, resulting in abnormal concentrations that can lead to endocrine disruption. This makes their determination in different matrices a vital part of pollutant management and control. Although advances in analytical instruments are constant, it has been determined that these instruments still require some sample preparation steps to be able to determine the occurrence of pollutants in the complex matrices in which they occur. Advances are still being made in sample preparation to ensure easier, selective, and sensitive analysis of complex matrices. Molecularly imprinted polymers (MIPs) have been termed as advanced solid-phase (SPE) materials for the selective extraction and preconcentration of hormones in complex matrices. This review explores the preparation and application of MIPs for the determination of steroid hormones in different sample types.
Multidisciplinary Approach for Hypothalamic Obesity in Children and Adolescents: A Preliminary Study
Authors: Tessaris Daniele , Matarazzo Patrizia , Tuli Gerdi , Tuscano Antonella , Rabbone Ivana , Spinardi Alessandra , Lezo Antonella , Fenocchio Giorgia , Buganza Raffaele , de Sanctis Luisa
Published: June 2021

Hypothalamic obesity (HO) is delineated by an inexorable weight gain in subjects with hypothalamic disorder (congenital or acquired). The aim of the present study was to evaluate the effect of a multidisciplinary approach on weight trend and metabolic outcome in children and adolescents with hypothalamic disease who were overweight or obese. Thirteen patients (aged 8.1–16.1 years) received a personalized diet, accelerometer-based activity monitoring, and psychological assessment. Height, weight, body mass index (BMI), and serum metabolic parameters were assessed at baseline (T0) and after six months (T1). Metformin was introduced at T1 in four subjects who were then re-evaluated after six months (T2). At T1, weight gain was significantly reduced compared with T0 (0.29 ± 0.79 kg/month vs. 0.84 ± 0.55 kg/month, p = 0.03), and weight standard deviation score (SDS) and BMI SDS did not change significantly, as serum metabolic parameters. The four subjects treated with metformin showed a reduction of weight SDS and BMI SDS at T2. In conclusion, patients treated with our multidisciplinary approach showed, after 6 months, favorable results characterized by decreased weight gain and stabilization of weight SDS and BMI SDS in a condition usually characterized by inexorable weight gain. However, further analysis, larger cohorts, and longer follow-up are needed to confirm these preliminary data.
Precipitation Climatology for the Arid Region of the Arabian Peninsula—Variability, Trends and Extremes
Authors: Patlakas Platon , Stathopoulos Christos , Flocas Helena , Bartsotas Nikolaos S. , Kallos George
Published: June 2021

The Arabian Peninsula is a region characterized by diverse climatic conditions due to its location and geomorphological characteristics. Its precipitation patterns are characterized by very low annual amounts with great seasonal and spatial variability. Moreover, extreme events often lead to flooding and pose threat to human life and activities. Towards a better understanding of the spatiotemporal features of precipitation in the region, a thirty-year (1986-2015) climatic analysis has been prepared with the aid of the state-of-the-art numerical modeling system RAMS/ICLAMS. Its two-way interactive nesting capabilities, explicit cloud microphysical schemes with seven categories of hydrometeors and the ability to handle dust aerosols as predictive quantities are significant advantages over an area where dust is a dominant factor. An extended evaluation based on in situ measurements and satellite records revealed a good model behavior. The analysis was performed in three main components; the mean climatic characteristics, the rainfall trends and the extreme cases. The extremes are analyzed under the principles of the extreme value theory, focusing not only on the duration but also on the intensity of the events. The annual and monthly rainfall patterns are investigated and discussed. The spatial distribution of the precipitation trends revealed insignificant percentage differences in the examined period. Furthermore, it was demonstrated that the eastern part and the top half of the western Arabian Peninsula presented the lowest risk associated with extreme events. Apart from the pure scientific interest, the present study provides useful information for different sectors of society and economy, such as civil protection, constructions and reinsurance.
Mitochondrial Genetic Diversity among Farmed Stocks of Oreochromis spp. (Perciformes, Cichlidae) in Madagascar
Authors: Hubert Nicolas , Pepey Elodie , Mortillaro Jean-Michel , Steinke Dirk , Andria-Mananjara Diana Edithe , de Verdal Hugues
Published: June 2021

The fast development of aquaculture over the past decades has made it the main source of fish protein and led to its integration into the global food system. Mostly originating from inland production systems, aquaculture has emerged as strategy to decrease malnutrition in low-income countries. The Nile tilapia (Oreochromis niloticus) was introduced to Madagascar in the 1950s, and is now produced nationally at various scales. Aquaculture mostly relies on fry harvested from wild populations and grow-out in ponds for decades. It has recently been diversified by the introduction of several fast-growing strains. Little is known how local genetic diversity compares to recently introduced strains, although high and comparable levels of genetic diversity have previously been observed for both wild populations and local stocks. Our study compares DNA barcode genetic diversity among eight farms and several strains belonging to three species sampled. DNA-based lineage delimitation methods were applied and resulted in the detection of six well differentiated and highly divergent lineages. A comparison of DNA barcode records to sequences on the Barcode of Life Data System (BOLD) helped to trace the origin of several of them. Both haplotype and nucleotide diversity indices highlight high levels of mitochondrial genetic diversity, with several local strains displaying higher diversity than recently introduced strains. This allows for multiple options to maintain high levels of genetic diversity in broodstock and provides more options for selective breeding programs.
The Impact of Linear Filter Preprocessing in the Interpretation of Permutation Entropy
Authors: Dávalos Antonio , Jabloun Meryem , Ravier Philippe , Buttelli Olivier
Published: June 2021

Permutation Entropy (PE) is a powerful tool for measuring the amount of information contained within a time series. However, this technique is rarely applied directly on raw signals. Instead, a preprocessing step, such as linear filtering, is applied in order to remove noise or to isolate specific frequency bands. In the current work, we aimed at outlining the effect of linear filter preprocessing in the final PE values. By means of the Wiener–Khinchin theorem, we theoretically characterize the linear filter’s intrinsic PE and separated its contribution from the signal’s ordinal information. We tested these results by means of simulated signals, subject to a variety of linear filters such as the moving average, Butterworth, and Chebyshev type I. The PE results from simulations closely resembled our predicted results for all tested filters, which validated our theoretical propositions. More importantly, when we applied linear filters to signals with inner correlations, we were able to theoretically decouple the signal-specific contribution from that induced by the linear filter. Therefore, by providing a proper framework of PE linear filter characterization, we improved the PE interpretation by identifying possible artifact information introduced by the preprocessing steps.