bluuebunny/crossref_metadata_2025 huggingface dataset:
Raw Material Surveys and Their Behavioral Implications in Highland Lesotho
Authors: Gregory Alex , Mitchell Peter , Pargeter Justin
Published: June 2023

Archaeologists have long considered climate change a primary mechanism behind human behavioral adaptations. The Lesotho highlands’ Afromontane and climatically extreme environments offer a unique opportunity to examine proposed correlations between topography, climate, and human behavior. Previous studies suggest that warmer temperatures allowed humans to expand their diet breadth and foraging range, whereas colder temperatures restricted humans to resources in riverine corridors. These studies used faunal and floral change as proxies to track changes in forager mobility but did not consider how differential access to stone resources affected human behaviors. To account for this gap, we conducted a survey for knappable rocks around the Sehonghong rock shelter in eastern Lesotho, recording the materials present and their size and shape in the modern environment. We compared the survey results to later Pleistocene (~ 22–11 ka cal. BP) lithic assemblages at Sehonghong to better understand whether archaeological patterns match modern knappable rock availability. Contrary to previous hypotheses, we find that past peoples at Sehonghong were not limited to exclusively riverine resources during colder conditions. We then used flake-to-core and noncortical-to-cortical flake ratios to track changes in mobility and knappable rock procurement patterns. The ratios remain constant up until the Late Glacial, ca. 14 ka cal. BP, when we see an increase in both flake-to-core and noncortical-to-cortical ratios, suggesting increased movement of stone out of Sehonghong. These conclusions show that resource procurement and mobility patterns are not solely dependent on climate change but may be driven by more complicated causal mechanisms such as increased interaction and the formation of social networks across the Lesotho highlands and beyond.
Productivity and Quality Variations in Sugar Beet Induced by Soil Application of K-Humate and Foliar Application of Biostimulants Under Salinity Condition
Authors: Nassar M. A. A. , El-Magharby Samia S. , Ibrahim Nada S. , Kandil Essam E. , Abdelsalam Nader R.
Published: June 2023

Abiotic stresses are the most major limitation to crop production, causing yield losses up to 50%, and consider the major challenges for production of crops. Thus, an experiment was conducted to determine how sugar beet would respond to soil application of K-humate rates and foliar application of biostimulants under salt stress. Split-plot design in three replicates was used in both seasons, where K-humate rates fertigation (control, 12, and 24 kg ha−1) allocated in the main plots, and different foliar application of BS arranged within the subplot (i.e., (1) water spray, (2) salicylic acid (SA) at the rate of 100 mg L−1, (3) fulvic acid (FA) at the rate of 1.2 kg ha−1, (4) hydroxyproline (HP) 1000 mg L−1, (5) SA at 100 mg L−1 + FA at 1.2 kg ha−1, (6) SA at 100 mg/L + HP at 1000 mg L−1, (7) FA at 1.2 kg ha−1 + HP at 1000 mg L−1, (8) SA 100 mg L−1 + FA 1.2 kg ha−1 + HP 1000 mg L−1) in the two seasons. The finding results revealed that soil application of K-humate, foliar application of BS, and their interaction significantly affected yield, growth, and quality characteristics of sugar beet under soil salinity, furthermore with soil application of K-humate at the rate of 24 kg ha−1 with foliar spray of FA + HP recorded the highest of growth traits, top, root, sucrose %, sugar yields/ha−1 and the lowest values of Na content in the juice and quality of sugar beet under the study conditions, Alexandria, Egypt.
Cost reduction in low-carbon hydrogen: effective but insufficient to mitigate carbon emissions
Authors: Wei Taoyuan , Glomsrød Solveig
Published: June 2023

Many countries have announced hydrogen promotion strategies to achieve net zero CO2 emissions around 2050. The cost of producing low-carbon (green and blue) hydrogen has been projected to fall considerably as production is scaled up, although more so for green hydrogen than for blue hydrogen. This article uses a global computable general equilibrium (CGE) model to explore whether the cost reduction of green and blue hydrogen production can mitigate the use of fossil fuels and related carbon emissions. The results show that cost reduction can raise low-carbon hydrogen consumption markedly in relative terms but marginally in absolute terms, resulting in a modest decrease in fossil fuel use and related carbon emissions. The cost reduction of low-carbon hydrogen slightly lowers the use of coal and gas but marginally increases the use of oil. If regional CO2 taxes are introduced the increase in green hydrogen production is considerably larger than in the case of low-carbon hydrogen cost reduction alone. However, if cost reduction in low-carbon hydrogen is introduced in addition to the CO2 tax the emissions from fossil fuels are only marginally reduced. Hence, synergy effects between the two measures on emissions are practically absent. A low-carbon hydrogen cost reduction alone is effective but insufficient to have a substantial climate impact. This study also calls for modeling development to capture special user preferences for low-carbon hydrogen related to climate mitigation when phasing in new energy carriers like hydrogen.
Using a Web Map Service to map Little Ice Age glacier extents at regional scales
Authors: Reinthaler Johannes , Paul Frank
Published: June 2023

Extending the record of glacier area changes into the past improves our understanding of climate change impacts. Although analogue maps showing historic glacier extents are abundant, digital outlines from before the satellite era are sparse as the digitisation of moraines and trimlines on freely available satellite images is challenging. With the now available very high-resolution images provided by Web Map Services (WMS), new doors are open for the precise digitisation. Here, we used the ESRI WMS to digitise Little Ice Age (LIA) glacier extents and present area changes since the LIA in four selected regions along with a detailed uncertainty analysis. We used modern glacier outlines as a starting point and additionally consulted Sentinel-2 images, the ArcticDEM and historic maps for interpretation. Dating records from the literature allowed calculating area change rates. In total, 493 LIA glaciers (4640 km2, now 891 ice bodies with 3590 km2) were digitised, yielding relative area changes of −20% (−0.14% a−1), −15% (−0.10% a−1), −26% (−0.16% a−1) and −61% (−0.19% a−1) for Alaska, Baffin Island, Novaya Zemlya and the tropics, respectively. The ESRI WMS images are a great asset to precisely map moraines and trimlines, but information about the timing of the related extents requires further sources.
Chemical, Biological, Radiological, and Nuclear Preparedness of Public Hospitals in Riyadh
Authors: Alahmari Ahmed A. , Khan Anas A.
Published: June 2023

Objectives:

Chemical, biological, radiological, and nuclear (CBRN) incidents are those that involve chemical or biological warfare agents or toxic radiological or nuclear materials. These agents can cause disasters intentionally or accidentally. Hospitals play a crucial role in handling CBRN disasters. This study aimed to assess the CBRN preparedness of government hospitals in Riyadh.

Methods:

A descriptive cross-sectional study was conducted across government hospitals in Riyadh. All government hospitals with more than 100 inpatient beds and an emergency department met the inclusion criteria. Hospital preparedness was assessed using an adaptation of the CBRNE (chemical, biological, radiological, nuclear, or explosive event) Plan Checklist. This adaptation was chosen due to the inclusion of explosive events in hospital disaster readiness, and its structural composition of key clinical guidelines necessary for a comprehensive disaster and readiness plan. Results were described in frequencies across several domains such as foundational considerations and planning which are used to assess plan preparedness using readiness tools, training, and awareness among staff members in accordance to a pre-established emergency plan, placed procedures and their implementation, and modules for preparing for a biological incident, a chemical incident, and a radiological or nuclear incident.

Results:

Of the 11 eligible hospitals, 10 participated in the study. Furthermore, CBRN considerations were included in the disaster plans of 7 hospitals. Drills had been conducted in collaboration with local agencies in only 2 hospitals. The staff had been trained to recognize the signs and symptoms of exposure to class (A) biological agents in less than half of the hospitals. Eight of the hospitals had antidotes and prophylactics to manage chemical incidents, but only half of them had radiation detection instruments. Personal protective equipment was available in all hospitals, but rapid access to stockpiles of medications was available in only half of them.

Conclusions:

Government hospitals in Riyadh demonstrated insufficient CBRN preparedness as per the CBRNE Plan Checklist. Overall, there was a lack of preemptive planning, application of pre-established policies and procedures, and adequate staff training. Furthermore, several hospitals had insufficient stockpiles of medications and in concrete plans on accessing government stockpiles in the case of an emergency. Therefore, their staffs should be trained to manage CBRN emergencies, and local drills should be conducted to improve their preparedness.
Legal & H.R. Centre and Centre for Reproductive Rights (on behalf of Tanzanian girls) v. Tanz. (Afr. Committee of Experts on the RTS & Welfare of the Child (ACERWC))
Authors: Celorio Rosa
Published: June 2023

On September 15, 2022, the African Committee of Experts on the Rights and Welfare of the Child (hereinafter ACERWC or Committee) issued its decision in the matter of Legal and Human Rights Centre and Centre for Reproductive Rights v. Tanzania. This case is related to the practice of forced pregnancy testing in schools in Tanzania and the later expulsion of girls who are found to be pregnant and/or married. The Communication was presented by the Legal and Human Rights Centre and the Centre for Reproductive Rights, on behalf of Tanzanian girls. This decision represents an important contribution to the line of international cases advancing the rights of girls under 18 years old, in particular in the areas of sexual and reproductive health and education, exemplified by increasing jurisprudence issued by regional human rights bodies.
Obstructed free-surface viscoplastic flow on an inclined plane
Authors: Hinton Edward M. , Hewitt Duncan R. , Hogg Andrew J.
Published: June 2023

The interaction of steady free-surface flows of viscoplastic material with a surface-piercing obstruction of square cross-section on an inclined plane is investigated theoretically. The flow thickness increases upstream of the obstruction and decreases in its lee. The flow depends on two dimensionless parameters: an aspect ratio that relates the flow thickness, the obstruction width and the plane inclination; and a Bingham number that quantifies the magnitude of the yield stress relative to the gravitationally induced stresses. Flows with a non-vanishing yield stress always form a static ‘dead’ zone in a neighbourhood of the upstream and downstream stagnation points. For relatively wide obstructions, a deep ‘ponded’ region develops upstream with a small dead zone, while the deflected flow reconnects over relatively long distances downstream. The depth of the upstream pond increases with both the dimensionless yield stress and width of the obstruction, while the unyielded dead zone varies primarily with the yield stress. Both are predicted asymptotically by balancing the volume flux of fluid into and out of the ponded region. When the obstruction is narrow, the perturbation to the depth of the oncoming flow is reduced. It exhibits fore–aft antisymmetry, while the dead zone is symmetric to leading order. Increasing the yield stress leads to larger dead zones that eventually encompass all of the upstream- and downstream-facing boundaries of the obstruction and fully divert the flow. Results for obstructions with circular and rhomboidal cross-sections are also presented and illustrate the effects of boundary shape on the properties of the steady flow.
The effectiveness of high-commitment human resource practices for employee knowledge sharing
Authors: Matošková Jana
Published: June 2023

Promoting knowledge sharing among employees is vital for companies whose competitive advantage is based on innovation. However, there is inadequate empirical evidence to show that human resource practices intensify knowledge sharing. Thus, this study examines whether high-commitment human resource management can boost knowledge sharing among staff members. A cross-sectional survey of permanent employees was conducted (N = 480) to examine the suitability of the secondary constructs ‘knowledge sharing in the organisation’ and ‘high-commitment human resource management’ using confirmatory factor analysis; their mutual relationship via structural equation modelling was also explored. The findings indicate that high-commitment human resource management increases knowledge dissemination in organisations. Additionally, they suggest that firms should concentrate on hiring selectively, providing autonomy and motivating work tasks to employees, setting practices for performance management and career management, and investing in employee training and development to support firm innovativeness.
Lean legacy, heavy heritage: family history of diabetes and its association with young adult body mass index
Authors: Kowaleski-Jones Lori , Zick Cathleen , Brown Barbara , Curtis David , Meeks Huong , Smith Ken
Published: June 2023

Substantial intergenerational transmission of diabetes mellitus (DM) risk exists. However, less is known regarding whether parental DM and DM among extended family members relate to adult offspring’s body mass index (BMI), and whether any of these associations vary by sex. Using data from the National Longitudinal Study of Youth 1997 cohort (NLSY97), we assess the sex-specific relationship between DM present in first-degree parents and second-degree relatives and BMI among the parents’ young adult offspring.

Multivariate regressions reveal a positive relationship between parental DM and young adults’ BMI for both daughters and sons, and the magnitude of coefficients is somewhat larger for the same-sex parent. Further, we observe that the link between parental DM and young adults’ BMI is strongest when both parents have diagnosed diabetes. In contrast, the relationship between second-degree relatives with DM and the respondent’s BMI is weaker and appears to be sex-specific, through same-sex parent and respondent. Logistic regressions show the association is especially strong when assessing how parental DM status relates to young adults’ obesity risk. These results generally persist when controlling for parental BMI. The findings of this study point to the need to better distinguish the role of shared family environments (e.g., eating and physical activity patterns) from shared genes in order to understand factors that may influence young adults’ BMI. Young adult offspring of parents with diabetes should be targeted for obesity prevention efforts in order to reduce their risks of obesity and perhaps diabetes.
Functional neuroimaging of resilience to trauma: convergent evidence and challenges for future research
Authors: Norbury Agnes , Seeley Saren H. , Perez-Rodriguez M. Mercedes , Feder Adriana
Published: June 2023

Resilience is broadly defined as the ability to adapt successfully following stressful life events. Here, we review functional MRI studies that investigated key psychological factors that have been consistently linked to resilience to severe adversity and trauma exposure. These domains include emotion regulation (including cognitive reappraisal), reward responsivity, and cognitive control. Further, we briefly review functional imaging evidence related to emerging areas of study that may potentially facilitate resilience: namely social cognition, active coping, and successful fear extinction. Finally, we also touch upon ongoing issues in neuroimaging study design that will need to be addressed to enable us to harness insight from such studies to improve treatments for – or, ideally, guard against the development of – debilitating post-traumatic stress syndromes.
Corticolimbic connectivity mediates the relationship between pubertal timing and mental health problems
Authors: Vijayakumar Nandita , Whittle Sarah , Silk Timothy J.
Published: June 2023

Background

Undergoing puberty ahead of peers (‘earlier pubertal timing’) is an important risk factor for mental health problems during early adolescence. The current study examined pathways between pubertal timing and mental health via connectivity of neural systems implicated in emotional reactivity and regulation (specifically corticolimbic connections) in 9- to 14-year-olds.

Method

Research questions were examined in the Adolescent Brain Cognitive Development (ABCD) Study, a large population representative sample in the United States. Linear mixed models examined associations between pubertal timing and resting-state corticolimbic connectivity. Significant connections were examined as potential mediators of the relationship between pubertal timing and mental health (withdrawn depressed and rule-breaking) problems. Exploratory analyses interrogated whether the family environment moderated neural risk patterns in those undergoing puberty earlier than their peers.

Results

Earlier pubertal timing was related to decreased connectivity between limbic structures (bilateral amygdala and right hippocampus) and the cingulo-opercular network, left amygdala and somatomotor (mouth) network, as well as between the left hippocampus and ventral attention network and visual network. Corticolimbic connections also mediated the relationship between earlier pubertal timing and increased withdrawn depressed problems (but not rule-breaking problems). Finally, parental acceptance buffered against connectivity patterns that were implicated in withdrawn depressed problems in those undergoing puberty earlier than their peers.

Conclusion

Findings highlight the role of decreased corticolimbic connectivity in mediating pathways between earlier pubertal timing and withdrawn depressed problems, and we present preliminary evidence that the family environment may buffer against these neural risk patterns during early adolescence.
ESTABLISHING A DYNASTY IN IDEOLOGY AND PRACTICE: THEAEDES VESTAEAUREI OF VESPASIAN
Authors: Arcenas Scott Lawin , Watson George C.
Published: June 2023

This paper uses a Flavian aureus type depicting theaedes Vestaeto explore both the ideological and the practical aspects of using coinage to propagate a dynasty. Firstly, we analyse the type from an iconographic perspective and argue that it should not be understood as a simple referent to the building itself, but rather as a complex interplay of different semantic units, intended to convey messages of dynastic harmony and security. Secondly, we use a die study to reconstruct the minting process and use our findings to elucidate the transformation of Roman coin production during the Flavian period. Taken together, the two parts of the paper demonstrate both the importance and the pay-offs of studying Roman coinage holistically, that is, with an integrated approach that combines iconographic and technical analysis. A catalogue of coins considered in the die study is provided as an appendix.
Radio wave propagation analysis with dominant path model using MIMO antenna element for vehicular base station application
Authors: Roja Vadlamudi , D. Sriram Kumar
Published: June 2023

A low-profile slant ±45° polarized antenna for a 4G/5G base station with a dominant path wave propagation model (DPM) is reported in this paper. For dual-polarization and wide impedance bandwidth, the two dipole radiators are crisscrossed and a square metallic ring is integrated with the dipole arms and screwed with the four shorting vias. The antenna operates in Band 40/41/42/43 with strong isolation and low ECC (Envelop Correlation Coefficient). Fabricated antenna results show that the predicted wideband antenna has 13.38% bandwidth in 4G, which is Band 40/41, and 29.33% bandwidth in 5G sub-6 GHz Band 42/43. The antenna has a consistent radiation pattern, a 40 dB XPD (Cross Pol Discrimination), and a beam width of 69.5° ±1.5° in the entire frequency band. Three base station sites were explored to deploy the radiation pattern of a dual-polarized antenna in the 3D EM (Electromagnetic Analysis) tool. To evaluate signal quality, strength, radio network planning, and wave propagation analysis by analyzing antenna performance in real-time scenarios with 64 and 256 QAM (Quadrature Amplitude Modulation) techniques. For the proposed four-stream MIMO (Multiple-Input-Multiple-Output) antenna arrangement at deployed cell sites for 4G/5G base station applications, the maximum downlink and uplink data rates are 3.826/2.8 Mbps and 1044/800 Mbps respectively.
Planting into a living cover crop alters preemergence herbicide dynamics and can reduce soybean yield
Authors: Nunes Jose J. , Arneson Nicholas J. , DeWerff Ryan P. , Ruark Matt , Conley Shawn , Smith Damon , Werle Rodrigo
Published: June 2023

Cereal rye cover crop (cereal rye) and preemergence (PRE) herbicides are becoming common practices for managing herbicide-resistant weeds in soybean production. Adopting these two practices in combination raises concerns regarding herbicide fate in soil, given that the cereal rye biomass can intercept the herbicide spray solution, preventing it from reaching the soil. Delaying cereal rye termination until soybean planting (planting green) optimizes biomass accumulation but might also increase PRE interception. To better understand the dynamics between cereal rye and PRE herbicides, a field experiment was conducted to evaluate two soil management practices (tillage and no-till) and two cereal rye termination practices in the planting-green system (glyphosate [1,260 g ae ha−1] and roller-crimper) on the spray deposition and fate of PRE herbicides and soybean yield. The spray deposition was assessed by placing water-sensitive paper cards on the soil surface before spraying the PRE herbicides (sulfentrazone [153 g ai ha−1] + S-metolachlor [1,379 g ai ha−1]). Herbicide concentration in soil (0 to 7.6 cm) was quantified 25 d after treatment (DAT). The presence of no-till stubble and cereal rye biomass reduced the spray coverage compared to tillage at PRE application, which reflected in a reduction in the concentration of both herbicides in soil 25 DAT. Soybean yield was reduced in all three years when the cereal rye was terminated with a roller-crimper but only reduced in one year when terminated with glyphosate. Our findings indicate that mainly cereal rye biomass reduced the concentration of PRE herbicides in the soil due to the interception of the spray solution during application. Although higher cereal rye biomass accumulation can provide better weed suppression according to the literature, farmers should be aware that the biomass can lower the concentration of PRE herbicides reaching the soil, thus intensifying field scouting to ensure that weed control is not being negatively affected.
Paleoseismology of a Major Crustal Seismogenic Source Near Mexico City: The Southern Border of the Acambay Graben
Authors: León‐Loya Rodrigo , Lacan Pierre , Ortuño María , Zúñiga F. Ramón , Štěpančíková Petra , Stemberk Jakub , Hernández Flores Ana Paula , Carrera Hernández Jaime J. , Sunyé‐Puchol Ivan , Aguirre‐Díaz Gerardo J. , Audin Laurence
Published: June 2023

The Trans‐Mexican Volcanic Belt is an active continental volcanic arc related to subduction along the Middle America trench. It is characterized by intra‐arc extension resulting into several major arc‐parallel active fault systems and tectonic basins. The Acambay graben, one of the largest of these basins, is located near Mexico City, in the central part of this province. In 1912, a M 6.9 earthquake ruptured the surface along the northern border of the graben together with at least two other faults. In this paper, we analyze the paleoseismic history of the southern border of the Acambay Graben, with new observations made in one natural outcrop and four paleoseismological trenches excavated across branches of the Venta de Bravo Fault at the site where it overlaps with the Pastores Fault. We present evidence of at least two paleo‐earthquakes that occurred between 12,190 ± 175 and 5,822 ± 87 cal year BP and between 647 ± 77 and 250 cal year BP. On one of these branches, we estimate a minimum slip‐rate value between 0.1 and 0.23 mm/year for the last 12 ka and a mean recurrence interval of 8.5 ± 3 ka. By considering several likely rupture lengths along the Venta de Bravo and Pastores faults, we calculated a maximum possible magnitude of Mw 7.01 ± 0.27. Finally, by correlating events recorded along different faults within the Acambay Graben, we discuss several possible rupture coalescent scenarios and related consequences for Mexico City.
Nickel Isotope Fractionation During Magmatic Differentiation
Authors: Yang Xi‐Ming , Wang Shui‐Jiong , Zhang Ya‐Wen , Dong Xu‐Han , Teng Fang‐Zhen , Helz Rosalind T. , Huang Jian , Li Xian‐Hua , Huang Shichun
Published: June 2023

The behavior of nickel (Ni) isotopes during magmatic differentiation is not adequately explored. Here, we find that tholeiitic rocks in the Kīlauea Iki (KI) lava lake, Hawai'i, show progressively lighter Ni isotopic compositions with increasing magmatic differentiation, whereas calc‐alkaline rocks from the thick Kamchatka arc (30–45 km), located at the convergent boundary of the Eurasian and Pacific plates show increasing Ni isotope values as MgO and Ni decrease. Forty‐three global intermediate‐felsic continental igneous rocks analyzed in this study display large Ni isotopic variations, with the Eoarchean samples having light Ni isotopic compositions that fall in the trend defined by the KI lavas, and the post‐Eoarchean samples showing systematically heavier Ni isotopic compositions overlapping those of Kamchatka arc rocks. The isotopic dichotomy results from the crystallization of isotopically heavy magnetite during low‐pressure differentiation of KI lavas, whereas the participation of sulfide separation that removes isotopically light Ni during high‐pressure differentiation of magmas traversing thick continental crust. Combined with Rhyolite‐MELTS and sulfur concentration at sulfide saturation simulations, we demonstrate that the Ni isotope fractionation during magmatic differentiation is mainly controlled by the tempo of magnetite crystallization and sulfide formation, which is a function of pressure, oxygen fugacity, and water activity. High‐pressure calc‐alkaline differentiation usually suppresses magnetite crystallization while stabilizing sulfide, leading to heavy Ni isotopic compositions for the evolved magmas, significantly deviating from the low‐pressure fractionation trend seen in the KI lavas. Ni isotopes can be used in the future as a tracer of magmatic differentiation and processes of continent formation and differentiation.
Primary cilia support cartilage regeneration after injury
Authors: Tao Dike , Zhang Lei , Ding Yunpeng , Tang Na , Xu Xiaoqiao , Li Gongchen , Niu Pingping , Yue Rui , Wang Xiaogang , Shen Yidong , Sun Yao
Published: June 2023

In growing children, growth plate cartilage has limited self-repair ability upon fracture injury always leading to limb growth arrest. Interestingly, one type of fracture injuries within the growth plate achieve amazing self-healing, however, the mechanism is unclear. Using this type of fracture mouse model, we discovered the activation of Hedgehog (Hh) signaling in the injured growth plate, which could activate chondrocytes in growth plate and promote cartilage repair. Primary cilia are the central transduction mediator of Hh signaling. Notably, ciliary Hh-Smo-Gli signaling pathways were enriched in the growth plate during development. Moreover, chondrocytes in resting and proliferating zone were dynamically ciliated during growth plate repair. Furthermore, conditional deletion of the ciliary core gene Ift140 in cartilage disrupted cilia-mediated Hh signaling in growth plate. More importantly, activating ciliary Hh signaling by Smoothened agonist (SAG) significantly accelerated growth plate repair after injury. In sum, primary cilia mediate Hh signaling induced the activation of stem/progenitor chondrocytes and growth plate repair after fracture injury.
Therapeutic blood-brain barrier modulation and stroke treatment by a bioengineered FZD4-selective WNT surrogate in mice
Authors: Ding Jie , Lee Sung-Jin , Vlahos Lukas , Yuki Kanako , Rada Cara C. , van Unen Vincent , Vuppalapaty Meghah , Chen Hui , Sura Asmiti , McCormick Aaron K. , Tomaske Madeline , Alwahabi Samira , Nguyen Huy , Nowatzke William , Kim Lily , Kelly Lisa , Vollrath Douglas , Califano Andrea , Yeh Wen-Chen , Li Yang , Kuo Calvin J.
Published: June 2023

Derangements of the blood-brain barrier (BBB) or blood-retinal barrier (BRB) occur in disorders ranging from stroke, cancer, diabetic retinopathy, and Alzheimer’s disease. The Norrin/FZD4/TSPAN12 pathway activates WNT/β-catenin signaling, which is essential for BBB and BRB function. However, systemic pharmacologic FZD4 stimulation is hindered by obligate palmitoylation and insolubility of native WNTs and suboptimal properties of the FZD4-selective ligand Norrin. Here, we develop L6-F4-2, a non-lipidated, FZD4-specific surrogate which significantly improves subpicomolar affinity versus native Norrin. In Norrin knockout (NdpKO) mice, L6-F4-2 not only potently reverses neonatal retinal angiogenesis deficits, but also restores BRB and BBB function. In adult C57Bl/6J mice, post-stroke systemic delivery of L6-F4-2 strongly reduces BBB permeability, infarction, and edema, while improving neurologic score and capillary pericyte coverage. Our findings reveal systemic efficacy of a bioengineered FZD4-selective WNT surrogate during ischemic BBB dysfunction, with potential applicability to adult CNS disorders characterized by an aberrant blood-brain barrier.
Rain triggers seasonal stratification in a temperate shelf sea
Authors: Jardine J. E. , Palmer M. , Mahaffey C. , Holt J. , Wakelin S. L. , Düsterhus A. , Sharples J. , Wihsgott J.
Published: June 2023

The North Atlantic Storm Track acts as a conveyor belt for extratropical cyclones that frequently deliver high winds and rainfall to northwest European shelf seas. Storms are primarily considered detrimental to shelf sea stratification due to wind-driven mixing countering thermal buoyancy, but their impact on shelf scale stratification cycles remains poorly understood. Here, we show that storms trigger stratification through enhanced surface buoyancy from rainfall. A multidecadal model confirms that rainfall contributed to triggering seasonal stratification 88% of the time from 1982 to 2015. Stratification could be further modulated by large-scale climate oscillations, such as the Atlantic Multidecadal Variability (AMV), with stratification onset dates being twice as variable during a positive AMV phase than a negative one. Further insights into how changing storm activity will impact shelf seas are discussed beyond the current view of increasing wind-driven mixing, with significant implications for marine productivity and ecosystem function.
Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulation
Authors: Choi Yong Mi , Ajjaji Dalila , Fleming Kaelin D. , Borbat Peter P. , Jenkins Meredith L. , Moeller Brandon E. , Fernando Shaveen , Bhatia Surita R. , Freed Jack H. , Burke John E. , Thiam Abdou Rachid , Airola Michael V.
Published: June 2023

Lipid droplets (LDs) are dynamic organelles that contain an oil core mainly composed of triglycerides (TAG) that is surrounded by a phospholipid monolayer and LD-associated proteins called perilipins (PLINs). During LD biogenesis, perilipin 3 (PLIN3) is recruited to nascent LDs as they emerge from the endoplasmic reticulum. Here, we analyze how lipid composition affects PLIN3 recruitment to membrane bilayers and LDs, and the structural changes that occur upon membrane binding. We find that the TAG precursors phosphatidic acid and diacylglycerol (DAG) recruit PLIN3 to membrane bilayers and define an expanded Perilipin-ADRP-Tip47 (PAT) domain that preferentially binds DAG-enriched membranes. Membrane binding induces a disorder to order transition of alpha helices within the PAT domain and 11-mer repeats, with intramolecular distance measurements consistent with the expanded PAT domain adopting a folded but dynamic structure upon membrane binding. In cells, PLIN3 is recruited to DAG-enriched ER membranes, and this requires both the PAT domain and 11-mer repeats. This provides molecular details of PLIN3 recruitment to nascent LDs and identifies a function of the PAT domain of PLIN3 in DAG binding.
Lipid droplets are a metabolic vulnerability in melanoma
Authors: Lumaquin-Yin Dianne , Montal Emily , Johns Eleanor , Baggiolini Arianna , Huang Ting-Hsiang , Ma Yilun , LaPlante Charlotte , Suresh Shruthy , Studer Lorenz , White Richard M.
Published: June 2023

Melanoma exhibits numerous transcriptional cell states including neural crest-like cells as well as pigmented melanocytic cells. How these different cell states relate to distinct tumorigenic phenotypes remains unclear. Here, we use a zebrafish melanoma model to identify a transcriptional program linking the melanocytic cell state to a dependence on lipid droplets, the specialized organelle responsible for lipid storage. Single-cell RNA-sequencing of these tumors show a concordance between genes regulating pigmentation and those involved in lipid and oxidative metabolism. This state is conserved across human melanoma cell lines and patient tumors. This melanocytic state demonstrates increased fatty acid uptake, an increased number of lipid droplets, and dependence upon fatty acid oxidative metabolism. Genetic and pharmacologic suppression of lipid droplet production is sufficient to disrupt cell cycle progression and slow melanoma growth in vivo. Because the melanocytic cell state is linked to poor outcomes in patients, these data indicate a metabolic vulnerability in melanoma that depends on the lipid droplet organelle.
NOTCH4ΔL12_16 sensitizes lung adenocarcinomas to EGFR-TKIs through transcriptional down-regulation of HES1
Authors: Zhang Bin , Dong Shaowei , Wang Jian , Huang Tuxiong , Zhao Pan , Xu Jing , Liu Dongcheng , Fu Li , Wang Lingwei , Wang Guangsuo , Zou Chang
Published: June 2023

Resistance to epidermal growth factor tyrosine kinase inhibitors (EGFR-TKI) remains one of the major challenges in lung adenocarcinoma (LUAD) therapy. Here, we find an increased frequency of the L12_16 amino acid deletion mutation in the signal peptide region of NOTCH4 (NOTCH4ΔL12_16) in EGFR-TKI-sensitive patients. Functionally, exogenous induction of NOTCH4ΔL12_16 in EGFR-TKI -resistant LUAD cells sensitizes them to EGFR-TKIs. This process is mainly mediated by the reduction of the intracellular domain of NOTCH4 (NICD4) caused by the NOTCH4ΔL12_16 mutation, which results in a lower localization of NOTCH4 in the plasma membrane. Mechanistically, NICD4 transcriptionally upregulates the expression of HES1 by competitively binding to the gene promoter relative to p-STAT3. Because p-STAT3 can downregulate the expression of HES1 in EGFR-TKI-resistant LUAD cells, the reduction of NICD4 induced by NOTCH4ΔL12_16 mutation leads to a decrease in HES1. Moreover, inhibition of the NOTCH4-HES1 pathway using inhibitors and siRNAs abolishes the resistance of EGFR-TKI. Overall, we report that the NOTCH4ΔL12_16 mutation sensitizes LUAD patients to EGFR-TKIs through transcriptional down-regulation of HES1 and that targeted blockade of this signaling cohort could reverse EGFR-TKI -resistance in LUAD, providing a potential approach to overcome resistance to EGFR-TKI -therapy.
Topological quadratic-node semimetal in a photonic microring lattice
Authors: Gao Zihe , Zhao Haoqi , Wu Tianwei , Feng Xilin , Zhang Zhifeng , Qiao Xingdu , Chiu Ching-Kai , Feng Liang
Published: June 2023

Graphene, with its two linearly dispersing Dirac points with opposite windings, is the minimal topological nodal configuration in the hexagonal Brillouin zone. Topological semimetals with higher-order nodes beyond the Dirac points have recently attracted considerable interest due to their rich chiral physics and their potential for the design of next-generation integrated devices. Here we report the experimental realization of the topological semimetal with quadratic nodes in a photonic microring lattice. Our structure hosts a robust second-order node at the center of the Brillouin zone and two Dirac points at the Brillouin zone boundary—the second minimal configuration, next to graphene, that satisfies the Nielsen–Ninomiya theorem. The symmetry-protected quadratic nodal point, together with the Dirac points, leads to the coexistence of massive and massless components in a hybrid chiral particle. This gives rise to unique transport properties, which we demonstrate by directly imaging simultaneous Klein and anti-Klein tunnelling in the microring lattice.
Fast synthesis of large-area bilayer graphene film on Cu
Authors: Zhang Jincan , Liu Xiaoting , Zhang Mengqi , Zhang Rui , Ta Huy Q. , Sun Jianbo , Wang Wendong , Zhu Wenqing , Fang Tiantian , Jia Kaicheng , Sun Xiucai , Zhang Xintong , Zhu Yeshu , Shao Jiaxin , Liu Yuchen , Gao Xin , Yang Qian , Sun Luzhao , Li Qin , Liang Fushun , Chen Heng , Zheng Liming , Wang Fuyi , Yin Wanjian , Wei Xiaoding , Yin Jianbo , Gemming Thomas , Rummeli Mark. H. , Liu Haihui , Peng Hailin , Lin Li , Liu Zhongfan
Published: June 2023

Bilayer graphene (BLG) is intriguing for its unique properties and potential applications in electronics, photonics, and mechanics. However, the chemical vapor deposition synthesis of large-area high-quality bilayer graphene on Cu is suffering from a low growth rate and limited bilayer coverage. Herein, we demonstrate the fast synthesis of meter-sized bilayer graphene film on commercial polycrystalline Cu foils by introducing trace CO2 during high-temperature growth. Continuous bilayer graphene with a high ratio of AB-stacking structure can be obtained within 20 min, which exhibits enhanced mechanical strength, uniform transmittance, and low sheet resistance in large area. Moreover, 96 and 100% AB-stacking structures were achieved in bilayer graphene grown on single-crystal Cu(111) foil and ultraflat single-crystal Cu(111)/sapphire substrates, respectively. The AB-stacking bilayer graphene exhibits tunable bandgap and performs well in photodetection. This work provides important insights into the growth mechanism and the mass production of large-area high-quality BLG on Cu.
A strong bimetal-support interaction in ethanol steam reforming
Authors: Meng Hao , Yang Yusen , Shen Tianyao , Liu Wei , Wang Lei , Yin Pan , Ren Zhen , Niu Yiming , Zhang Bingsen , Zheng Lirong , Yan Hong , Zhang Jian , Xiao Feng-Shou , Wei Min , Duan Xue
Published: June 2023

The metal-support interaction (MSI) in heterogeneous catalysts plays a crucial role in reforming reaction to produce renewable hydrogen, but conventional objects are limited to single metal and support. Herein, we report a type of RhNi/TiO2 catalysts with tunable RhNi-TiO2 strong bimetal-support interaction (SBMSI) derived from structure topological transformation of RhNiTi-layered double hydroxides (RhNiTi-LDHs) precursors. The resulting 0.5RhNi/TiO2 catalyst (with 0.5 wt.% Rh) exhibits extraordinary catalytic performance toward ethanol steam reforming (ESR) reaction with a H2 yield of 61.7%, a H2 production rate of 12.2 L h−1 gcat−1 and a high operational stability (300 h), which is preponderant to the state-of-the-art catalysts. By virtue of synergistic catalysis of multifunctional interface structure (Rh-Niδ−-Ov-Ti3+; Ov denotes oxygen vacancy), the generation of formate intermediate (the rate-determining step in ESR reaction) from steam reforming of CO and CHx is significantly promoted on 0.5RhNi/TiO2 catalyst, accounting for its ultra-high H2 production.
Microstructural and functional plasticity following repeated brain stimulation during cognitive training in older adults
Authors: Antonenko Daria , Fromm Anna Elisabeth , Thams Friederike , Grittner Ulrike , Meinzer Marcus , Flöel Agnes
Published: June 2023

The combination of repeated behavioral training with transcranial direct current stimulation (tDCS) holds promise to exert beneficial effects on brain function beyond the trained task. However, little is known about the underlying mechanisms. We performed a monocenter, single-blind randomized, placebo-controlled trial comparing cognitive training to concurrent anodal tDCS (target intervention) with cognitive training to concurrent sham tDCS (control intervention), registered at ClinicalTrial.gov (Identifier NCT03838211). The primary outcome (performance in trained task) and secondary behavioral outcomes (performance on transfer tasks) were reported elsewhere. Here, underlying mechanisms were addressed by pre-specified analyses of multimodal magnetic resonance imaging before and after a three-week executive function training with prefrontal anodal tDCS in 48 older adults. Results demonstrate that training combined with active tDCS modulated prefrontal white matter microstructure which predicted individual transfer task performance gain. Training-plus-tDCS also resulted in microstructural grey matter alterations at the stimulation site, and increased prefrontal functional connectivity. We provide insight into the mechanisms underlying neuromodulatory interventions, suggesting tDCS-induced changes in fiber organization and myelin formation, glia-related and synaptic processes in the target region, and synchronization within targeted functional networks. These findings advance the mechanistic understanding of neural tDCS effects, thereby contributing to more targeted neural network modulation in future experimental and translation tDCS applications.
Asymmetry of carbon sequestrations by plant and soil after forestation regulated by soil nitrogen
Authors: Hong Songbai , Ding Jinzhi , Kan Fei , Xu Hao , Chen Shaoyuan , Yao Yitong , Piao Shilong
Published: June 2023

Forestation is regarded as an effective strategy for increasing terrestrial carbon sequestration. However, its carbon sink potential remains uncertain due to the scarcity of large-scale sampling data and limited knowledge of the linkage between plant and soil C dynamics. Here, we conduct a large-scale survey of 163 control plots and 614 forested plots involving 25304 trees and 11700 soil samples in northern China to fill this knowledge gap. We find that forestation in northern China contributes a significant carbon sink (913.19 ± 47.58 Tg C), 74% of which is stored in biomass and 26% in soil organic carbon. Further analysis reveals that the biomass carbon sink increases initially but then decreases as soil nitrogen increases, while soil organic carbon significantly decreases in nitrogen-rich soils. These results highlight the importance of incorporating plant and soil interactions, modulated by nitrogen supply in the calculation and modelling of current and future carbon sink potential.
Two RhoGEF isoforms with distinct localisation control furrow position during asymmetric cell division
Authors: Montembault Emilie , Deduyer Irène , Claverie Marie-Charlotte , Bouit Lou , Tourasse Nicolas J. , Dupuy Denis , McCusker Derek , Royou Anne
Published: June 2023

Cytokinesis partitions cellular content between daughter cells. It relies on the formation of an acto-myosin contractile ring, whose constriction induces the ingression of the cleavage furrow between the segregated chromatids. Rho1 GTPase and its RhoGEF (Pbl) are essential for this process. However, how Rho1 is regulated to sustain furrow ingression while maintaining correct furrow position remains poorly defined. Here, we show that during asymmetric division of Drosophila neuroblasts, Rho1 is controlled by two Pbl isoforms with distinct localisation. Spindle midzone- and furrow-enriched Pbl-A focuses Rho1 at the furrow to sustain efficient ingression, while Pbl-B pan-plasma membrane localization promotes the broadening of Rho1 activity and the subsequent enrichment of myosin on the entire cortex. This enlarged zone of Rho1 activity is critical to adjust furrow position, thereby preserving correct daughter cell size asymmetry. Our work highlights how the use of isoforms with distinct localisation makes an essential process more robust.
Insights into substitution strategy towards thermodynamic and property regulation of chemically recyclable polymers
Authors: Tu Yi-Min , Gong Fu-Long , Wu Yan-Chen , Cai Zhongzheng , Zhu Jian-Bo
Published: June 2023

The development of chemically recyclable polymers serves as an attractive approach to address the global plastic pollution crisis. Monomer design principle is the key to achieving chemical recycling to monomer. Herein, we provide a systematic investigation to evaluate a range of substitution effects and structure−property relationships in the ɛ-caprolactone (CL) system. Thermodynamic and recyclability studies reveal that the substituent size and position could regulate their ceiling temperatures (Tc). Impressively,M4equipped with atert-butyl group displays aTcof 241 °C. A series of spirocyclic acetal-functionalized CLs prepared by a facile two-step reaction undergo efficient ring-opening polymerization and subsequent depolymerization. The resulting polymers demonstrate various thermal properties and a transformation of the mechanical performance from brittleness to ductility. Notably, the toughness and ductility of P(M13) is comparable to the commodity plastic isotactic polypropylene. This comprehensive study is aimed to provide a guideline to the future monomer design towards chemically recyclable polymers.
Critical roles of metal–ligand complexes in the controlled synthesis of various metal nanoclusters
Authors: Kim Ji Soo , Chang Hogeun , Kang Sungsu , Cha Seungwoo , Cho Hanguk , Kwak Seung Jae , Park Namjun , Kim Younhwa , Kang Dohun , Song Chyan Kyung , Kwag Jimin , Hahn Ji-Sook , Lee Won Bo , Hyeon Taeghwan , Park Jungwon
Published: June 2023

Metal nanoclusters (NCs), an important class of nanoparticles (NPs), are extremely small in size and possess quasi-molecular properties. Due to accurate stoichiometry of constituent atoms and ligands, NCs have strong structure-property relationship. The synthesis of NCs is seemingly similar to that of NPs as both are formed by colloidal phase transitions. However, they are considerably different because of metal-ligand complexes in NC synthesis. Reactive ligands can convert metal salts to complexes, actual precursors to metal NCs. During the complex formation, various metal species occur, having different reactivity and fraction depending on synthetic conditions. It can alter their degree of participation in NC synthesis and the homogeneity of final products. Herein, we investigate the effects of complex formation on the entire NC synthesis. By controlling the fraction of various Au species showing different reactivity, we find that the extent of complex formation alters reduction kinetics and the uniformity of Au NCs. We demonstrate that this concept can be universally applied to synthesize Ag, Pt, Pd, and Rh NCs.