bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of the 2020–2022 Crises on EU Countries’ Independence from Energy Imports, Particularly from Russia
Authors: Rokicki Tomasz , Bórawski Piotr , Szeberényi András
Published: September 2023

The main objective of this article is to evaluate the repercussions of the crises resulting from the COVID-19 pandemic and the war in Ukraine on changes to the European Union’s reliance on energy raw material imports, particularly from Russia. Furthermore, several specific objectives are outlined: to ascertain the EU’s energy balance and any alterations therein, to identify changes in the concentration of energy raw material imports among EU countries during the crises, to determine the level and fluctuations in energy raw material import dependence within the EU and individual member states throughout the crises, and lastly, to assess changes in the concentration of energy raw material imports from Russia in EU countries during the crises. The research encompassed all 27 EU member states as of 31 December 2022, and the examination period spanned 2010 to 2022. The information sources include relevant literature on the subject and data sourced from Eurostat. In conducting the analysis and presenting the data, several methodologies are employed, including descriptive, tabular, and graphical techniques. Additionally, quantitative measures such as the Gini structure concentration coefficient and concentration analysis via the Lorenz curve are utilized. In the initial phase, an analysis of changes to the energy balance of the entire EU in the period before and during the COVID-19 pandemic and the war in Ukraine was carried out. Changes to the concentration of energy imports in EU countries during crises are also shown. Secondly, dependence on imports of individual energy resources for the entire EU was identified. Various changes in this relationship during crises are shown. If the dependence of the entire EU on the import of individual groups of energy resources from Russia were identified, changes in individual EU countries in this respect are indicated. Finally, the changes in the concentration of energy imports from Russia in the EU countries during the crises are shown. During the COVID-19 pandemic, the EU experienced a slight reduction in dependence on energy raw material imports. However, this reduction was short-lived and mainly attributed to the decrease in energy demand caused by the pandemic. Conversely, the war in Ukraine had a substantial impact, leading to a notable surge in energy raw material imports as a result of stockpiling efforts and the need to safeguard against further disruptions in the energy market. Notably, the EU’s reliance on energy imports from Russia was already considerable. Following the outbreak of the conflict in Ukraine, imports of oil, petroleum products, and solid fossil fuels experienced significant decline due to imposed sanctions. Nonetheless, restrictions on natural gas imports were comparatively less severe. In 2023, imports of crude oil instead of petroleum products practically ceased, while restrictions in the case of natural gas were not so drastic.
Production of Microbial Lipids by Saitozyma podzolica Zwy2-3 Using Corn Straw Hydrolysate, the Analysis of Lipid Composition, and the Prediction of Biodiesel Properties
Authors: Feng Shunli , Guo Yihan , Ran Yulu , Yang Qingzhuoma , Cao Xiyue , Yang Huahao , Cao Yu , Xu Qingrui , Qiao Dairong , Xu Hui , Cao Yi
Published: September 2023

Although Saitozyma podzolica Zwy2-3 can use the enzymatic hydrolysate of corn stalks treated with an ammonium carbonate-steam explosion (EHCS-ACSE) as a substrate for lipid accumulation, the inefficient conversion of sugars from EHCS-ACSE into lipids necessitates the further optimization of fermentation parameters. Response surface design was used to optimize the primary fermentation parameters. Under the optimized conditions of the reducing sugar concentration of 89.44 g/L, yeast extract concentration of 3.88 g/L, rotational speed of 219 rpm, and incubation time of 122 h, the maximum lipid production achieved 11.45 g/L, which was 2.28 times higher than the results of the previous study. In addition, lipid profiling showed the presence of four fatty acid methyl esters, with the highest percentage being 61.84% oleic acid, followed by 21.53% palmitic acid, 13.05% stearic acid, and 3.58% linoleic acid. It is noteworthy that the composition and relative abundance of microbial lipids remained constant under different culture conditions. The characteristics of Zwy2-3 biodiesel, such as the iodine value (62.09), cetane number (59.29), density (0.87 g/cm3), and oxidation stability (35.53), meet the international standards (ASTM D6751-02 and EN 14214) for biodiesel. The present study further demonstrated that S. podzolica Zwy2-3 can efficiently utilize EHCS-ACSE for microbial lipid accumulation, and its lipids have favorable qualities that make them suitable for biodiesel production.
Energy Resilience in Telecommunication Networks: A Comprehensive Review of Strategies and Challenges
Authors: Cabrera-Tobar Ana , Grimaccia Francesco , Leva Sonia
Published: September 2023

As telecommunication networks become increasingly critical for societal functioning, ensuring their resilience in the face of energy disruptions is paramount. This review paper comprehensively analyzes strategies and challenges associated with achieving energy resilience in telecommunication networks. It explores various aspects, including policies, energy backup systems, renewable energy integration, and energy management techniques. This paper discusses how these strategies can be implemented to build resilience across three phases: preparedness (referring to the proactive measures taken in advance), response and relief, recovery and reconstruction. Additionally, it discusses the challenges associated with implementing energy resilience measures, taking into account policies, sustainability and environment, and climate change. By synthesizing existing research and identifying research gaps, this review paper aims to provide insights into the state-of-the-art practices and future directions for enhancing energy resilience in telecommunications, enabling robust and uninterrupted communication services.
A Critical Review of Recent Progress in Global Water Reuse during 2019–2021 and Perspectives to Overcome Future Water Crisis
Authors: Abou-Shady Ahmed , Siddique Muhammad Saboor , Yu Wenzheng
Published: September 2023

The exacerbation of the global water crisis due to an increase in global population, industrialization, urbanization, and agricultural activities, along with global climate change and limited water resources, makes water reuse inevitable in all continents. By 2030, global water consumption may grow to ~160% of the currently available volume. This study reviews recently published articles (2019–2021) to explore global case studies of water reuse and discusses future perspectives by country based on a literature survey on water reuse. There are 17 obstacles reported worldwide regarding water reuse (e.g., the properties and low amounts of treated water, regulations, financial challenges, etc.) and 10 advantages of utilizing reused water in various fields (e.g., overcoming the global water crisis, improving the economy, benefiting the industrial sector, etc.). The concept of reusing water has been accepted by countries in almost every continent (e.g., Australia, Europe, Asia, Africa, South America, and North America); the technical findings from different countries are summarized in this study. The water reuse scenario is not restricted to countries with limited water supply and can be applied to those with sufficient water resources (e.g., Canada and Brazil have also implemented water reuse policies). Water reuse can be utilized by human beings via indirect and direct potable recycling, as well as in agriculture, textile, construction, hotel, groundwater recharge, and aquaculture industries. However, a standard guideline for the application of reclaimed water at a global scale is unavailable. Several perspectives have been suggested for the future utilization of reclaimed water worldwide as an effort to secure and ensure the sustainability of existing natural water resources. Lastly, water reuse may be considered a potential alternative for reducing the burden on water resources in the future.
Pesticide Application as a Risk Factor/Behaviour for Workers’ Health: A Systematic Review
Authors: Moreira Andreia , da Silva Manuela Vieira
Published: September 2023

The main objective of this review was to determine the main risks that agricultural workers are exposed to during pesticide application, which may have a harmful effect on their health and on public health. This systematic review was based on the PRISMA guidelines. A search for articles was conducted in the Medline/PubMed, ScienceDirect, and Web of Science databases. Fifteen articles were selected considering their assessment of agricultural workers’ knowledge, perceptions, attitudes, practices, and behaviours, identifying the main risks and risk factors for disease associated with the unsafe handling of pesticides. The main risk factors identified were age, education, pesticide safety training, farming experience, and contact with other farmers/intermediaries resulting in pesticide access. The most frequent risk behaviour was an application of pesticides without personal protective equipment (PPE), incorrect disposal of empty packaging and waste, and undervaluation of label information, as well as other unsafe practices. Multidisciplinary and more effective training must be delivered to enhance pesticide-safe usage. This will empower workers to adopt more conscious and safer behaviours while using pesticides.
Development of a Real-Time Continuous Measurement System for Tree Radial Direction
Authors: Zhang Qianjia , Sun Yuanjing , Zheng Xinyu , Zhang Shusheng , Fang Luming
Published: September 2023

Tree diameter at breast height (DBH) is the most fundamental factor in modelling tree growth, but current DBH measurement instruments mainly focus on instantaneous acquisition, making it difficult to measure tree growth continuously and accurately. In this study, we propose a wireless sensing network that can transmit data in signal-free environments, and combine sensor and computer technologies to develop a real-time continuous measurement system for tree radials, which has the advantages of working in real-time, being low-cost and stable, and enabling high-precision. It can be applied to the DBH measurement of trees in the range of 50 mm–380 mm, with a measurement accuracy of 0.001 mm. Additionally, whole-point sampling, conducted 24 h per day, integrates DBH data measurement, transmission, storage and visualization analysis. After measuring in the field for a year, it initially reveals the change in DBH within the test area within that year. This study provides a scientific basis for researching the microscopic growth pattern of trees and establishing a tree growth model, which will be further optimised and improved in terms of appearance structure, communication and power supply in the future.
Microbial Fuel Cell Using a Novel Ionic Liquid-Type Membrane–Cathode Assembly for Animal Slurry Treatment and Fertilizer Production
Authors: Iniesta-López Eduardo , Hernández-Fernández Adrián , Garrido Yolanda , Ieropoulos Ioannis A. , Hernández-Fernández Francisco José
Published: September 2023

The implementation of a microbial fuel cell for wastewater treatment and bioenergy production requires a cost reduction, especially when it comes to the ion exchange membrane part and the catalysts needed for this purpose. Ionic liquids in their immobilized phase in proton exchange membranes and non-noble catalysts, as alternatives to conventional systems, have been intensively investigated in recent years. In the present study, a new microbial fuel cell technology, based on an ionic liquid membrane assembly for CoCu mixed oxide catalysts, is proposed to treat animal slurry. The new low-cost membrane–cathode system is prepared in one single step, thus simplifying the manufacturing process of a membrane–cathode system. The novel MFCs based on the new low-cost membrane–cathode system achieved up to 51% of the power reached when platinum was used as a catalyst. Furthermore, the removal of organic matter in suspension after 12 days was higher than that achieved with a conventional system based on the use of platinum catalysts. Moreover, struvite, a precipitate consisting of ammonium, magnesium, and phosphate, which could be used as a fertilizer, was recovered using this membrane–cathode system.
Impact of Mechanical Stirring and Percolate Recirculation on the Performances of Dry Anaerobic Digestion
Authors: Zhang Zhikai , Chang Shengqiang , Zhao Shengyong , Liu Peng , Qian Yanan , Li Wangliang
Published: September 2023

Dry anaerobic digestion (DAD) is an attractive method for simultaneous organic waste disposal and bioenergy recovery. DAD has the problems of low methane yields, low reaction rates, and easy inhibition due to its limited mass transfer and heat transfer. In this work, two methods of mechanical stirring and percolate recirculation were compared regarding their capacities of improving the mass transfer and enhancing the performances of DAD in batch experiments with sorghum stalks as a substrate. The cumulative biogas yield and system stability were investigated when the stirring linear velocity was 0 cm/s, 22 cm/s, 44 cm/s, 66 cm/s, and 88 cm/s. When the stirring linear velocity was 88 cm/s, the cumulative biogas yield and methane content were highest. The computational fluid dynamics (CFD) simulation indicated that the shearing force near the stirring shaft was largest. When the linear velocity of the stirring paddle was 88 cm/s, the shearing force at a radial distance close to center was about −140 N/m2. When the ratio of the material stacking height to the reactor diameter (H/D) was 3:2, the AD showed the best performance. A higher material stacking height promoted the contact between the microorganisms and the substrate and enhanced the biogas production. By combining percolate recirculation and mechanical stirring, the cumulative biogas yield increased by 28% compared with the static DAD process because of the promotion of mass transfer in the DAD.
Innovative and Sustainable Composite Material for the Seismic and Energetic Upgrade of Historic Masonry Buildings
Authors: Pugliese Dora , Alecci Valerio , Romano Rosa , Stipo Gianfranco , De Stefano Mario , Nanni Antonio
Published: September 2023

Usually, energy and structural improvements for historic masonry buildings are addressed separately using distinct methods and protocols. This paper covers an integrated assessment of new composite materials to reduce the seismic vulnerability of historic masonry buildings while complying with sustainable conservation requirements, emissions’ reduction, and energy savings. Firstly, this study focused on selecting suitable thermal mortars that could serve as the base material for the innovative composite. Subsequently, the mechanical characteristics of these mortars were examined by subjecting them to compressive and three-point bending tests. Dynamic thermo-hygrometric simulations were conducted using commercially available software to check the energy performance of the composite material when used on walls of existing masonry buildings. The thermal mortar that exhibited the most favorable mechanical and thermal properties was subsequently reinforced with a basalt fabric. A composite sample was assembled and subjected to direct tensile testing to determine its stress–strain behavior.
Unravelling Metabolic Heterogeneity of Chinese Baijiu Fermentation in Age-Gradient Vessels
Authors: Miao Zijian , Bai Yu , Wang Xinlei , Han Chao , Wang Bowen , Li Zexia , Sun Jinyuan , Zheng Fuping , Zhang Yuhang , Sun Baoguo
Published: September 2023

Fermentation vessels affect the characteristics of food fermentation; however, we lack an approach to identify the biomarkers indicating fermentation. In this study, we applied metabolomics and high-throughput sequencing analysis to reveal the dynamic of metabolites and microbial communities in age-gradient fermentation vessels for baijiu production. Furthermore, we identified 64 metabolites during fermentation, and 19 metabolites significantly varied among the three vessels (p < 0.05). Moreover, the formation of these 19 metabolites were positively correlated with the core microbiota (including Aspergillus, Saccharomyces, Lactobacillus, and Bacillus). In addition, ethyl lactate or ethyl acetate were identified as the biomarkers for indicating the metabolism among age-gradient fermentation vessels by BP-ANN (R2 > 0.40). Therefore, this study combined the biological analysis and predictive model to identify the biomarkers indicating metabolism in different fermentation vessels, and it also provides a potential approach to assess the profiling of food fermentations.
Glycated Casein by TGase-Type Exerts Protection Potential against DSS-Induced Colitis via Inhibiting TLR4/NF-κB Signaling Pathways in C57BL/6J Mice
Authors: Chu Hui , Liu Weiling , Zhao Cong , Yin Tong , Shi Jia , Zhang Wei
Published: September 2023

Glycation by transglutaminase (TGase)-type could effectively improve the structure and functional properties of proteins. However, the influence on intestinal inflammation or the underlying mechanisms has not been investigated. The goal of this research was to compare the bioactivities between glycated casein generated from the TGase-catalyzed reaction and oligochitosan as well as casein using a mouse model of dextran sulfate sodium (DSS)-induced intestinal inflammation to examine the protective effects and the underlying mechanism of glycated casein on intestinal inflammation. Eight groups of C57BL/6 mice were randomly assigned in this study: Control group: standard diet for 35 days; Model group: standard diet for 28 days and then colitis induction; Pretreated groups: different levels (200, 400, 800 mg/kg BW) of casein or glycated casein for 28 days before colitis induction. The mice were drinking water containing a 3% DSS solution for seven days of mice to cause colitis. The results indicated that glycated casein and casein at 200–800 mg/kg BW all relieved DSS-induced weight loss, reduced disease activity index (DAI) score, alleviated colon length shortening, weakened the destruction of colonic mucosal structure, decreased serum LPS, and MPO, IL-1β, IL-6 and TNF-α levels in serum and colon, as well as regulated the expression of proteins involved in the TLR4/NF-κB signaling pathway in a concentration-dependent manner. Glycated caseinate showed a better protective effect against DSS-induced colitis than casein, highlighting that the TGase-type glycation of proteins as a potential functional food ingredient might be a helpful method for gut health.
Co-Encapsulation of Multiple Polyphenols in Plant-Based Milks: Formulation, Gastrointestinal Stability, and Bioaccessibility
Authors: Zheng Bingjing , Zhou Hualu , McClements David Julian
Published: September 2023

Plant-based milk is particularly suitable for fortification with multiple nutraceuticals because it contains both hydrophobic and hydrophilic domains that can accommodate molecules with different polarities. In this study, we fortified soymilk with three common polyphenols (curcumin, quercetin, and resveratrol) using three pH-driven approaches. We compared the effectiveness of these three different approaches for co-encapsulating polyphenols. The gastrointestinal fate of the polyphenol-fortified soymilks was then studied by passing them through a simulated mouth, stomach, and small intestine, including the stability and bioaccessibility of polyphenols. All three pH-driven approaches were suitable for co-encapsulating multiple polyphenols at a high encapsulation efficiency, especially for the curcumin and resveratrol. The polyphenol-loaded delivery systems exhibited similar changes in particle size, charge, stability, and bioaccessibility as they passed through the mouth, stomach, and intestinal phases. The bioaccessibility of the co-encapsulated polyphenols was much greater than that of crystallized polyphenols dispersed in water. The poor bioaccessibility of the crystallized polyphenols was attributed to their low solubility in water, which made them more difficult to solubilize within mixed micelles. This study underscores the feasibility of pH-driven approaches for encapsulating a variety of polyphenols into the same plant-based delivery system. These fortified plant-based milks may therefore be designed to provide specific health benefits to consumers.
Designing an Informative App for Neurorehabilitation: A Feasibility and Satisfaction Study by Physiotherapists
Authors: Sánchez-Rodríguez María Teresa , Pinzón-Bernal Mónica Yamile , Jiménez-Antona Carmen , Laguarta-Val Sofía , Sánchez-Herrera-Baeza Patricia , Fernández-González Pilar , Cano-de-la-Cuerda Roberto
Published: September 2023

Background: New technologies have gained popularity, especially the use of mobile phone applications, in neurorehabilitation. The aim of this paper was (1) to develop a free mobile application (NeurorehAPP) that provides information about and helps to select the appropriate mobile application related to a list of neurological disorders (cognitive impairment, Alzheimer’s disease, Parkinson‘s disease, multiple sclerosis, traumatic brain injury, stroke, cerebral palsy, muscular dystrophy, spina bifida, and facial paralysis), based on different objectives such as healthy habits, information, assessment, and treatment; and (2) to assess the feasibility, acceptability, and degree of satisfaction by physiotherapists after using NeurorehAPP for a minimum of three months. Methods: A free application was created to work with the Android® operating system. The degree of satisfaction and acceptance with the application was assessed with an adaptation of the Customer Satisfaction Questionnaire through a survey via email applied to physiotherapists from hospitals and neurological rehabilitation centers in Spain after using the application. Results: NeurorehAPP includes a total of 131 apps. A total of 121 physiotherapists completed a satisfaction survey. The total sample showed 85.41% satisfaction with the service provided by the app and 86.41% overall satisfaction with NeurorehAPP. Conclusions: NeurorehAPP is a free, intuitive, and friendly app used with the Android® operating system that allows the selection of the most appropriate app according to the type of user, neurological disorder, objective, and FDA criteria. Physiotherapists showed a high degree of satisfaction and acceptance with NeurorehAPP.
Knowledge and Attitudes towards Epilepsy of Croatian General Student Population and Biomedical Students: A Cross-Sectional Study
Authors: Žuvela Tomislav , Filipović-Grčić Branka , Rušić Doris , Leskur Dario , Modun Darko , Čohadžić Tin , Bukić Josipa , Šešelja Perišin Ana
Published: September 2023

Epilepsy causes a significant burden to patients as it is linked with various somatic and psychiatric comorbidities, social issues, impaired quality of life, and increased mortality. Improving the population’s knowledge and attitudes about epilepsy patients could be beneficial as it could raise social awareness and lead to more social support for patients. For those reasons, a survey-based cross-sectional study was conducted to determine Croatian students’ knowledge and attitudes toward epilepsy. A previously developed survey questionnaire was adapted for the Croatian setting and distributed online to the students (n = 544). Croatian students generally had positive attitudes towards people with epilepsy (median score 28.0, interquartile range 29.0–26.0, with the minimum possible score being 0.0 and the maximum 30.0), with the female gender (B (male) = 0.664 (95% CI −1.158, −0.170), p = 0.009), biomedical education (B (other) = −0.442, (95% CI −0.823, −0.061), p = 0.023), and personal experience in the form of witnessing the seizure (B = 0.519 (95% CI 0,098, 0.940), p = 0.016) as predictors of more favorable attitudes. Overall knowledge was satisfactory concerning most items, with the exception of first aid measures and risk factors. Educational intervention targeting bio-medical students and other students who might, in their future professional lives, be responsible for people suffering from epilepsy is needed to improve the gaps in their knowledge.
Improving Alzheimer’s Disease Classification in Brain MRI Images Using a Neural Network Model Enhanced with PCA and SWLDA
Authors: Ahmad Irshad , Siddiqi Muhammad Hameed , Alhujaili Sultan Fahad , Alrowaili Ziyad Awadh
Published: September 2023

The examination of Alzheimer’s disease (AD) using adaptive machine learning algorithms has unveiled promising findings. However, achieving substantial credibility in medical contexts necessitates a combination of notable accuracy, minimal processing time, and universality across diverse populations. Therefore, we have formulated a hybrid methodology in this study to classify AD by employing a brain MRI image dataset. We incorporated an averaging filter during preprocessing in the initial stage to reduce extraneous details. Subsequently, a combined strategy was utilized, involving principal component analysis (PCA) in conjunction with stepwise linear discriminant analysis (SWLDA), followed by an artificial neural network (ANN). SWLDA employs a combination of forward and backward recursion methods to choose a restricted set of features. The forward recursion identifies the most interconnected features based on partial Z-test values. Conversely, the backward recursion method eliminates the least correlated features from the same feature space. After the extraction and selection of features, an optimized artificial neural network (ANN) was utilized to differentiate the various classes of AD. To demonstrate the significance of this hybrid approach, we utilized publicly available brain MRI datasets using a 10-fold cross-validation strategy. The proposed method excelled over existing state-of-the-art systems, attaining weighted average recognition rates of 99.35% and 96.66%, respectively, across all the datasets.
Somatic Embryogenesis and Plant Regeneration from Stem Explants of Pomegranate
Authors: Wang Jingting , Xia Xinhui , Qin Gaihua , Tang Jingwen , Wang Jun , Zhu Wenhao , Qian Ming , Li Jiyu , Cui Guangrong , Yang Yuchen , Qian Jingjing
Published: September 2023

Plant regeneration through somatic embryogenesis provides a solution for maintaining and genetically improving crop or fruit varieties with desirable agronomic traits. For the fruit tree pomegranate (Punica granatum L.), despite some successful applications, the existing somatic embryogenesis protocols are limited by low availability of explants and susceptibility to browning. To address these problems, in this study, we developed an effective system for induction of high-vigor pomegranate somatic embryos derived from stem explants. The usage of stem explants breaks through the difficulty in obtaining material, thus making our system suitable for widespread commercial production. To enhance the performance of our system, we identified the optimal explants, subculture cycles and combination of basal media and plant growth regulators for each step. The results showed that inoculating stem explants onto a Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-benzylaminopurine (6-BA) and 1.0 mg/L 1-naphthaleneacetic acid (NAA) achieved the best induction rate and growth status of pomegranate calli (induction rate = ~72%), and MS medium containing 0.5 mg/L 6-BA and 1.0 mg/L NAA was the optimal condition for the induction of embryogenic calli and somatic embryos (induction rate = ~74% and 79%, respectively). The optimal subculture period for embryogenic calli was found to be 30–35 days. Strong roots were then induced in the developed somatic embryo seedlings, which survived and grew well after transplantation to the natural environment, indicating the good vitality of the induced pomegranate somatic embryos. Together, our system provides a solution to mass somatic embryo induction and plant regeneration of pomegranate and lays a foundation for future genetic transformation and bioengineering improvement of pomegranate with favorable agronomic traits.
Boron Isotopes in Fresh Surface Waters in a Temperate Coastal Setting
Authors: Peritore Brooke N. , Rasbury E. Troy , Wooton Kathleen M. , Wright Carrie C. , Downs Deanna M. , Iorga Anastasia , Letscher Shannon L.
Published: September 2023

The results from a four-year study of a freshwater pond on Long Island, NY, USA, do not point to a single source of boron (and by proxy other elements including nutrients) in this system. However, boron data from samples associated with this pond can be explained by mixing between average precipitation (weighted average δ11B = 22.7) in the area and the local sources of boron, both natural and anthropogenic. This multiyear study provided the opportunity to see both yearly and seasonal differences. One algae sample from the pond showed significant fractionation and enrichment in light boron relative to the water and suggests algae may act as a boron sink. This type of biological fractionation could explain an observed down-gradient trend to heavier boron isotope values in pond water, which corresponds to the slight reduction in boron concentration seen in 2021. However, the trend was subdued in the following year, likely due to differences in the water flow rates and/or rate of algal growth. An opposite trend was seen with depth in the water, where δ11B showed a positive correlation to boron concentration, which increased with depth from the surface of the pond. This gradient may be explained by the stratification of the pond with a heavy source concentrating in the bottom waters. The bottom water composition was consistent with goose feces (δ11B = 25.8) or the addition of chemicals from the application of rock salt to local roads in winter. Surprisingly, boron from seawater (average δ11B = 39.8) did not appear to have a direct impact on Setauket Pond, other than its influence on precipitation, providing heavy δ11B and very low boron concentrations.
Assessing Terrestrial Water Storage Variations in Southern Spain Using Rainfall Estimates and GRACE Data
Authors: Pardo-Igúzquiza Eulogio , Montillet Jean-Philippe , Sánchez-Morales José , Dowd Peter A. , Luque-Espinar Juan Antonio , Darbeheshti Neda , Rodríguez-Tovar Francisco Javier
Published: September 2023

This paper investigates the relationship between rainfall, groundwater and Gravity Recovery and Climate Experiment (GRACE) data to generate regional-scale estimates of terrestrial water storage variations in the Andalucía region of southern Spain. These estimates can provide information on groundwater depletion (caused by periods of low rainfall or droughts) and groundwater recovery. The spatial distribution of groundwater bodies in southern Spain is complex and current in situ groundwater monitoring methods are deficient, particularly in terms of obtaining representative samples and in implementing and maintaining groundwater monitoring networks. The alternative approach proposed here is to investigate the relationship between precipitation time series and changes in the terrestrial water storage estimated from GRACE observations. The results were validated against the estimated fluctuation in regional groundwater. The maximum correlation between the mean groundwater level and the GRACE observations is 0.69 and this occurs at a lag of one month because the variation in gravity is immediate, but rainfall water requires around one month to travel across the vadose zone before it reaches the groundwater table. Using graphical methods of accumulated deviations from the mean, we show that, in general, groundwater storage follows the smooth, multi-year trends of terrestrial water storage but with less short-term trends; the same is true of rainfall, for which the local trends are more pronounced. There is hysteresis-like behaviour in the variations in terrestrial water storage and in the variations of groundwater. In practical terms, this study shows that, despite the abnormal dryness of the Iberian Peninsula during the 2004–2010 drought, the depleted groundwater storage in Andalucía recovered almost to its pre-drought level by 2016. In addition, groundwater storage and terrestrial water storage show very similar trends but with a delay in the groundwater trend.
Impact of COVID-19 Pandemic on Children’s Fundamental Motor Skills: A Study for the Taiwanese Preschoolers Teachers
Authors: Cheng Shu-Yu , Tai Hsia-Ling , Wang Tsung-Teng
Published: September 2023

The outbreak of the COVID-19 pandemic has resulted in reduced opportunities for children to engage in fundamental motor skills [FMS]. This prolonged inactivity and restriction of play can have serious consequences for children’s physical and mental health. The purpose of this study was to explore teaching strategies during the pandemic, whether there were differences in children’s motor development, and the differences in the implementation of physical movement courses before and during the pandemic from the perspective of preschool teachers. This study was a retrospective study using an internet survey, and participants comprised 2337 preschool teachers. The statistical methodology of this study included descriptive statistics, the dependent t-test, and the independent t-test. The results showed that regardless of the time, frequency, activity intensity, and frequency of outdoor courses, the results from before the pandemic was better than those taken during the pandemic. Only the “frequency of implementing physical movement courses indoors every week” had not been affected by the pandemic. This study also obtained the performance of “children’s fitness”, “overall performance of physical movement ability”, “stability movement skills”, “locomotor movement skills”, and “manipulative movement skills”. All were better before the pandemic than during the pandemic. During the COVID-19 pandemic, mixed-age classes performed better than same-age classes in terms of frequency, time, intensity, outdoor course implementation, and physical fitness. Public schools performed better than private schools in terms of frequency, time, intensity, outdoor course implementation, and fundamental motor skills performance. Private schools implemented physical movement courses indoors every week, which was more than public schools. Excepting the frequency of implementing physical movement courses indoors every week, fewer than schools with five classes performed better than those who had more than schools with six classes. Finally, rural schools were better than urban schools in the implementation of outdoor courses and fundamental motor skills performance. Therefore, we suggest that in response to the pandemic, teachers should further improve their professionalism and use diversified teaching methods, and guide students to be willing to learn and improve their skill performance.
A Survey of Vaping Use, Perceptions, and Access in Adolescents from South-Central Texas Schools
Authors: Gilmore Bretton A. , Gilmore Corbyn M. , Reveles Kelly R. , Koeller Jim M. , Spoor Jodi H. , Flores Bertha E. , Frei Christopher R.
Published: September 2023

Despite efforts to dissuade major manufacturers and retailers from marketing and selling vape products to adolescents, the practice of vaping continues to increase in this population. Few studies have assessed adolescent perceptions of vaping, access to vaping, and use of vaping, and most rely, at least in part, on inferential conclusions drawn from data on smoking traditional combustible cigarettes. A novel electronic survey was created to assess the use of vapes, perceptions of vaping, and access to vaping among a convenience sample of adolescents (ages 12–20 years) in eleven schools in South-Central Texas from May to August 2021. The students’ perceived threat of negative health outcomes due to vaping was calculated based on questions soliciting perceptions of severity (perceived danger) and susceptibility (perceived likelihood of illness). Trends were identified using descriptive and bivariate statistical tests. A total of 267 respondents were included; 26% had tried vaping. A majority (63%) did not believe vaping and smoking were synonymous. Most (70%) thought it was easy to obtain supplies and (76%) vape before and after (88%) or even during (64%) school. Respondents who vaped had a 34% lower perceived threat when compared to respondents who did not vape. In this sample of adolescents from South-Central Texas, one in four reported that they had tried vaping. Easy access to vapes and misperceptions regarding the safety of vaping might create a false sense of security with respect to vaping as an alternative to smoking, particularly among those who reported vaping, and is likely contributing to the increased use of vapes.
Sex-Specific Dysconnective Brain Injuries and Neuropsychiatric Conditions such as Autism Spectrum Disorder Caused by Group B Streptococcus-Induced Chorioamnionitis
Authors: Vancolen Seline , Ayash Taghreed , Allard Marie-Julie , Sébire Guillaume
Published: September 2023

Global health efforts have increased against infectious diseases, but issues persist with pathogens like Group B Streptococcus (GBS). Preclinical studies have elaborated on the mechanistic process of GBS-induced chorioamnionitis and its impact on the fetal programming of chronic neuropsychiatric diseases. GBS inoculation in rodents demonstrated the following: (i) silent and self-limited placental infection, similar to human chorioamnionitis; (ii) placental expression of chemokines attracting polymorphonuclear (PMN) cells; (iii) in vitro cytokine production; (iv) PMN infiltration in the placenta (histologic hallmark of human chorioamnionitis), linked to neurobehavioral impairments like cerebral palsy and autism spectrum disorders (ASD); (v) upregulation of interleukin-1β (IL-1β) in the placenta and fetal blood, associated with higher ASD risk in humans; (vi) sex-specific effects, with higher IL-1β release and PMN recruitment in male placenta; (vii) male offspring exhibiting ASD-like traits, while female offspring displayed attention deficit and hyperactivity disorder (ADHD)-like traits; (viii) IL-1 and/or NF-kB blockade alleviate placental and fetal inflammation, as well as subsequent neurobehavioral impairments. These findings offer potential therapeutic avenues, including sex-adapted anti-inflammatory treatment (e.g., blocking IL-1; repurposing of FDA-approved IL-1 receptor antagonist (IL-1Ra) treatment). Blocking the IL-1 pathway offers therapeutic potential to alleviate chorioamnionitis-related disabilities, presenting an opportunity for a human phase II RCT that uses IL-1 blockade added to the classic antibiotic treatment of chorioamnionitis.
The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer’s Disease
Authors: Jovanovic Macura Irena , Zivanovic Ana , Perovic Milka , Ciric Jelena , Major Tamara , Kanazir Selma , Ivkovic Sanja
Published: September 2023

Cerebral amyloid angiopathy (CAA) is characterized by amyloid β (Aβ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of Aβ is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The Aβ accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 M and 12 M 5xFAD and 12 M WT retinas. The increase in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 M WT retinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiological (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.
Gintonin-Induced Wound-Healing-Related Responses Involve Epidermal-Growth-Factor-like Effects in Keratinocytes
Authors: Won Kyung-Jong , Lee Rami , Choi Sun-Hye , Kim Ji-Hun , Hwang Sung-Hee , Nah Seung-Yeol
Published: September 2023

Epidermal growth factor (EGF) receptor activation and related downstream signaling pathways are known to be one of the major mechanisms of the proliferation and migration of keratinocytes. The heparin-binding EGF-like growth factor (HB-EGF) binds to EGF receptors and stimulates keratinocyte proliferation and migration. Gintonin, a novel ginseng compound, is a lysophosphatidic acid (LPA) receptor ligand. Gintonin has skin-wound-healing effects. However, the underlying mechanisms for these gintonin actions remain unclear. In this study, we aimed to elucidate the involvement of EGFRs in gintonin-induced wound repair in HaCaT keratinocytes. In this study, a water-soluble tetrazolium salt-based assay, a modified Boyden chamber migration assay, and immunoblotting were performed. Gintonin increased EGF receptor activation in HaCaT cells. However, the gintonin-induced phosphorylation of the EGF receptor was markedly reduced via treatment with the LPA inhibitor Ki16425 or the EGF receptor inhibitor erlotinib. Gintonin-enhanced proliferation and migration were blocked by the EGF receptor inhibitors (erlotinib and AG1478). Additionally, gintonin stimulated the expression and release of HB-EGF in HaCaT cells. EGF receptor inhibitors blocked gintonin-enhanced HB-EGF expression. These results indicate that the wound-healing effects of gintonin are closely related to the collaboration between EGF receptor activation and HB-EGF release-mediated downstream signaling pathways.
Re-Analysis of the Widely Used Recombinant Murine Cytomegalovirus MCMV-m157luc Derived from the Bacmid pSM3fr Confirms Its Hybrid Nature
Authors: Cordsmeier Arne , Bednar Christopher , Kübel Sabrina , Bauer Larissa , Ensser Armin
Published: September 2023

Murine cytomegalovirus (MCMV), and, in particular, recombinant virus derived from MCMV-bacmid pSM3fr, is widely used as the small animal infection model for human cytomegalovirus (HCMV). We sequenced the complete genomes of MCMV strains and recombinants for quality control. However, we noticed deviances from the deposited reference sequences of MCMV-bacmid pSM3fr. This prompted us to re-analyze pSM3fr and reannotate the reference sequence, as well as that for the commonly used MCMV-m157luc reporter virus. A correct reference sequence for this frequently used pSM3fr, containing a repaired version of m129 (MCK-2) and the luciferase gene instead of ORF m157, was constructed. The new reference also contains the original bacmid sequence, and it has a hybrid origin from MCMV strains Smith and K181.
Catecholamines Promote Ovarian Cancer Progression through Secretion of CXC-Chemokines
Authors: Kim Hyun Jung , Chang Ha Kyun , Lee Yul Min , Heo Kyun
Published: September 2023

Considerable evidence has accumulated in the last decade supporting the notion that chronic stress is closely related to the growth, metastasis, and angiogenesis of ovarian cancer. In this study, we analyzed the conditioned media in SKOV3 ovarian cancer cell lines treated with catecholamines to identify secreted proteins responding to chronic stress. Here, we observed that epinephrine and norepinephrine enhanced the secretion and mRNA expression of CXC-chemokines (CXCL1, 2, 3, and 8). Neutralizing antibodies to CXCL8 and CXCL8 receptor (CXCR2) inhibitors significantly reduced catecholamine-mediated invasion of SKOV3 cells. Finally, we found that the concentration of CXCL1 and CXCL8 in the plasma of ovarian cancer patients increased with stage progression. Taken together, these findings suggest that stress-related catecholamines may influence ovarian cancer progression through the secretion of CXC-chemokines.
Diabetic Endothelial Cell Glycogen Synthase Kinase 3β Activation Induces VCAM1 Ectodomain Shedding
Authors: Brishti Masuma Akter , Raghavan Somasundaram , Lamar Kennedy , Singh Udai P. , Collier Daniel M. , Leo M. Dennis
Published: September 2023

Soluble cell adhesion molecules (sCAMs) are secreted ectodomain fragments of surface adhesion molecules, ICAM1 and VCAM1. sCAMs have diverse immune functions beyond their primary function, impacting immune cell recruitment and activation. Elevated sVCAM1 levels have been found to be associated with poor cardiovascular disease (CVD) outcomes, supporting VCAM1’s role as a potential diagnostic marker and therapeutic target. Inhibiting sVCAM1’s release or its interaction with immune cells could offer cardioprotection in conditions such as diabetes. Membrane-bound surface adhesion molecules are widely expressed in a wide variety of cell types with higher expression in endothelial cells (ECs). Still, the source of sCAMs in the circulation is not clear. Hypothesizing that endothelial cells (ECs) could be a potential source of sCAMs, this study investigated whether dysfunctional EC signaling mechanisms during diabetes cause VCAM1 ectodomain shedding. Our results from samples from an inducible diabetic mouse model revealed increased sVCAM1 plasma levels in diabetes. Protein analysis indicated upregulated VCAM1 expression and metalloproteases ADAM10 and ADAM17 in diabetic ECs. ADAMs are known for proteolytic cleavage of adhesion molecules, contributing to inflammation. GSK3β, implicated in EC VCAM1 expression, was found to be activated in diabetic ECs. GSK3β activation in control ECs increased ADAM10/17 and VCAM1. A GSK3β inhibitor reduced active GSK3β and VCAM1 ectodomain shedding. These findings suggest diabetic ECs with elevated GSK3β activity led to VCAM1 upregulation and ADAM10/17-mediated sVCAM1 shedding. This mechanism underscores the potential therapeutic role of GSK3β inhibition in reducing the levels of circulating sVCAM1. The complex roles of sCAMs extend well beyond CVD. Thus, unraveling the intricate involvement of sCAMs in the initiation and progression of vascular disease, particularly in diabetes, holds significant therapeutic potential.